bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Diversity and Molecular Characterization of Worldwide Prairie Grass (Bromus catharticus Vahl) Accessions Using SRAP Markers
Authors: Yi Limei , Dong Zhixiao , Lei Yu , Zhao Junming , Xiong Yanli , Yang Jian , Xiong Yi , Gou Wenlong , Ma Xiao
Published: October 2021

Prairie grass (Bromus catharticus Vahl) is an important grass species that could be used in the production systems of certified seed and high-quality forage for grazing ruminants. In the present research, a sequence-related amplified polymorphism (SRAP) marker was employed to detect the genetic variability and structure of 80 prairie grass accessions from all over the world. Altogether, 460 reliable bands were amplified from 47 SRAP primer pairs with 345 (75%) polymorphic bands. The average values of discrimination power (DP) and polymorphic information content (PIC) were 0.753 and 0.317, respectively. Both the UPGMA clustering and PCoA analyses grouped the 80 accessions into five clusters, whereas the STRUCTURE analysis showed that 80 prairie grass accessions possessed three genetic memberships (K = 3). The results of the Mantel test showed that the distance matrix has a moderately positive correlation between the morphological and molecular data sets (r = 0.524). A poor genetic differentiation (Fst = 0.045) was discovered among the six geo-groups of accessions. Besides, the highest intragroup genetic diversity was found in the North America group (He = 0.335). This study provides a genetic structure and diversity case for prairie grass, and supplies new clues for the study and utilization of prairie grass.
Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro
Authors: Trifunović-Momčilov Milana , Milošević Snežana , Marković Marija , Đurić Marija , Jevremović Slađana , Dragićević Ivana Č. , Subotić Angelina R.
Published: October 2021

The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll (Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general, salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium. The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2 transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations >50-mM NaCl, the total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together, the results of this study partly explained the mode of centaury plant adaptations to salt stress in vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic pigment contents can be a very useful non-destructive screening method in order to discriminate susceptible and resistant plant species/lines to salt stress conditions.
A Modified Approach for the Ultrasound-Guided Quadratus Lumborum Block in Dogs: A Cadaveric Study
Authors: Viscasillas Jaime , Terrado Jose , Marti-Scharfhausen Reyes , Castiñeiras Diego , Esteve Vicente , Clancy Niamh , Redondo Jose Ignacio
Published: October 2021

Ultrasound-guided quadratus lumborum block (QLB) is a locoregional technique described in canine cadavers. The aim of this study was to assess a modified approach to QLB to minimise potential complications such as abdominal organ puncture. Nine canine cadavers were included and were positioned in lateral recumbency. An ultrasound-guided QLB was performed on each side. The probe was placed in the transverse position over the lumbar muscles just caudal to the last rib, and a needle was advanced in-plane from a dorso-lateral to a ventro-medial. A volume of 0.2 mL kg−1 of a mixture of iomeprol and methylene blue was injected. Computed tomography (CT) and dissection were performed to evaluate the spreading. Success was defined as staining of the nerve with a length of more than 0.6 cm. Potential complications such as intra-abdominal, epidural, or intravascular spreading of the mixture were also assessed. The CT images showed a T13 to L7 vertebra distribution, with a median of 5 (3–6). Dissection showed staining of the nerves from T13 to L4, with a median of 3 (2–5). No complications were found. This modified approach to QLB is safe and shows similar results to the previous studies in canine carcass.
Spatial–Temporal Distribution of Megamouth Shark, Megachasma pelagios, Inferred from over 250 Individuals Recorded in the Three Oceans
Authors: Yu Chi-Ju , Joung Shoou-Jeng , Hsu Hua-Hsun , Lin Chia-Yen , Hsieh Tzu-Chi , Liu Kwang-Ming , Yamaguchi Atsuko
Published: October 2021

The megamouth shark (Megachasma pelagios) is one of the rarest shark species in the three oceans, and its biological and fishery information is still very limited. A total of 261 landing/stranding records were examined, including 132 females, 87 males, and 42 sex unknown individuals, to provide the most detailed information on global megamouth shark records, and the spatial–temporal distribution of M. pelagios was inferenced from these records. The vertical distribution of M. pelagios ranged 0–1203 m in depth, and immature individuals were mostly found in the waters shallower than 200 m. Mature individuals are not only able to dive deeper, but also move to higher latitude waters. The majority of M. pelagios are found in the western North Pacific Ocean (>5° N). The Indian and Atlantic Oceans are the potential nursery areas for this species, immature individuals are mainly found in Indonesia and Philippine waters. Large individuals tend to move towards higher latitude waters (>15° N) for foraging and growth from April to August. Sexual segregation of M. pelagios is found, females tend to move to higher latitude waters (>30° N) in the western North Pacific Ocean, but males may move across the North Pacific Ocean.
Association between Fatty Acid Composition, Cryotolerance and Fertility Competence of Progressively Motile Bovine Spermatozoa
Authors: Kogan Tanya , Grossman Dahan Dana , Laor Ronit , Argov-Argaman Nurit , Zeron Yoel , Komsky-Elbaz Alisa , Kalo Dorit , Roth Zvi
Published: October 2021

An association between progressive motility (PM) and spermatozoa fertility competence has been suggested. However, the mechanism that underlies PM is not clear enough. We examined physiological characteristics and fatty acid composition of fresh spermatozoa with high and low PM. Additional analysis of fatty acid composition and structural characteristics was performed on spermatozoa samples with high and low progressively motile spermatozoa’s survival (PMSS), i.e., the ratio between the proportion of progressively motile spermatozoa after and before cryopreservation. Finally, a fertility field trial was conducted to examine the association between the number of PM spermatozoa within the insemination straw post thawing and conception rate. Analysis of fresh spermatozoa revealed a higher omega-6 to omega-3 ratio in ejaculates with low PM relative to those with high PM (p < 0.01). The proportion of polyunsaturated fatty acids was higher in low-PMSS fresh samples (p < 0.05) relative to their high-PMSS counterparts. Fresh samples with high-PMSS expressed a higher mitochondrial membrane potential (p < 0.05) and a higher proportion of viable cells that expressed reactive oxygen species (ROS; p < 0.05). Post-thawing evaluation revealed a reduced proportion of progressively motile sperm, with a prominent effect in samples with high PM relative to low PM, defined before freezing (p < 0.01). No differences in spermatozoa mitochondrial membrane potential or ROS level were found post-thawing. A fertility study revealed a positive correlation between the number of progressively motile spermatozoa within a standard insemination straw and conception rate (p < 0.05). Considering these, the bull PMSS is suggested to be taken into account at the time of straw preparation.
Factors Associated with Fatality in Ontario Thoroughbred Racehorses: 2003–2015
Authors: Physick-Sheard Peter , Avison Amanda , Sears William
Published: October 2021

Ontario’s Alcohol and Gaming Commission records equine racing fatalities through its Equine Health Program. The present study examined all Thoroughbred fatalities from 2003 to 2015, inclusive, to identify associations. Official records and details of fatalities were combined in multivariable logistic regression modelling of 236,386 race work-events (433 fatalities), and 459,013 workout work-events (252 fatalities). Fatality rates were 2.94/1000 race starts (all fatalities) and 1.96/1000 (breakdowns only) with an overall rate of 2.61% or 26.1 fatalities/1000 horses. Comparison with published reports reveals rates to be high. Musculoskeletal injury was the predominant complaint and there was a high incidence of horses dying suddenly. Liability was high for young horses early in the season with a differential according to sex and whether a male horse was gelded. Horses undertaking repeated workouts had a higher liability and liability was higher in workouts for horses switching from dirt/synthetic to turf racing and for young horses in sprints. Race distance was not significant but high fatality rates in some large field, distance races combined with effects of age and workload identified groups at particular risk. As field size increased, fatality liability increased for early-finishing horses. Findings suggest jockey strategy could be an important factor influencing fatalities. Probability of fatality declined over the study period. Findings indicate that rapid accumulation of workload in animals early in their preparation is likely to be damaging. Fatality fell toward the end of a season and for horses with a long career history of successful performance; however, horses not exhibiting this robustness and staying power represent the population of greatest concern. Associations may be characterised as representing sources of stress, current or cumulative, and identifying at-risk animals on this basis may be as productive as targeting specific, discrete mechanisms suspected to contribute to individual fatalities.
Usefulness of Selected Acute-Phase Proteins in the Postsurgical Monitoring of Arthroscopy and Splint Bone Removal in Horses
Authors: Przewoźny Maciej , Senderska-Płonowska Magdalena , Rząsa Anna , Wierzbicki Heliodor , Borkowski Jacek , Swagemakers Jan-Hein , Żak-Bochenek Agnieszka , Stefaniak Tadeusz
Published: October 2021

Background: Arthroscopy and splint bone removal are the common orthopedic procedures in horses. Estimation of the dynamics of acute phase proteins in postoperative monitoring seems to be interesting diagnostic approach. The aim of the study was to investigate changes in the concentrations of plasma inflammatory markers—fibrinogen, haptoglobin, and protease inhibitors—following orthopedic surgery in horses. The study involved 114 horses, divided into two study groups undergoing: arthroscopy (41 horses) and splint bone removal (13 horses). The control group consisted of 60 healthy horses. The blood was collected before the surgery and 24, 48, 72 h, 5, 7, 10, 14 and 28 days after the surgery. Plasma fibrinogen, serum haptoglobin and proteinase inhibitors were measured. Results: In non-complicated cases of arthroscopy and splint bone removal, fibrinogen and haptoglobin increased stepwise from 24 h, achieved the maximum level at 72 h and returned to preoperative levels after 10–14 days. In one complicated case after arthroscopy surgery the marked increase in fibrinogen and haptoglobin concentrations was observed 24 h earlier than standard parameters of inflammation Conclusion: The study shows the evolution of APPs after arthroscopy and splint bone removal in 28 days postsurgery period and in the case of one complicated case of arthroscopy.
Colonization of Urban Habitats: Tawny Owl Abundance Is Conditioned by Urbanization Structure
Authors: Pagaldai Nerea , Arizaga Juan , Jiménez-Franco María V. , Zuberogoitia Iñigo
Published: October 2021

Natural habitats are being altered and destroyed worldwide due to urbanization, leading to a decrease in species abundance and richness. Nevertheless, some species, including tawny owls, have successfully colonized this novel habitat. Consequences at the population level have not been described; thus, our main objective was to describe the effects that urban structure have on the tawny owl population at local and landscape levels. Data were obtained from 527 survey points over 7 months in a large-scale owl survey in the Basque Country (northern Spain) in 2018. At the local scale, the interaction between forest and urban cover affected tawny owl abundance, the optimum being in medium forested areas. The interaction between urban cover and clumpiness index (urban patch distribution) showed a generally negative effect. At the landscape scale, its abundance decreased in complex-shaped urban patches and when distance between them was greater. In conclusion, at the local scale, when a minimal forest structure is present in urbanized areas, the species can exploit it. At the landscape scale, it prefers smaller urban towns to cities. Thinking ahead, the current tendency toward “green capitals” should benefit tawny owl populations.
Therapeutic Potential of Sitafloxacin as a New Drug Candidate for Helicobacter Eradication in Korea: An In Vitro Culture-Based Study
Authors: Choi Youn I , Lee Sung Min , Chung Jun-Won , Kim Kyoung Oh , Kwon Kwang An , Kim Yoon Jae , Kim Jung Ho , Lee Sun Mi , Jeong Jin-Yong , Park Dong Kyun
Published: October 2021

Background: Increased prevalence of antibiotic resistance to Helicobacter pylori (H. pylori) infection worldwide has driven the search for a new therapeutic candidate. Recently, sitafloxacin, a novel 4-quinolone agent, has emerged as a new therapeutic option for H. pylori eradication, in Japan. However, data on its efficacy for H. pylori eradication in Korea are limited. Therefore, we aimed to investigate the therapeutic potential of sitafloxacin as a first-line treatment for patients with Helicobacter infection through gastric tissue culture-based studies. Materials and Methods: We prospectively enrolled treatment-naïve patients with H. pylori infection who visited the Gil Medical Center between March 2015 and March 2018. After obtaining written informed consent from patients, a total of 121 H. pylori strains were collected. We tested the susceptibility of these strains to sitafloxacin, and other antibiotics for Helicobacter eradication, including clarithromycin (CLR), metronidazole (MTZ), amoxicillin (AMX), tetracycline (TET), levofloxacin (LEV), and ciprofloxacin (CIP) using the agar dilution technique. The minimum inhibitory concentration (MIC) of these antibiotics against H. pylori strains were determined. Results: None of the H. pylori strains obtained were resistant to sitafloxacin (MIC > 1, n = 0), while other conventional eradication drugs including CLR, MTZ, AMX, and TET showed 24.8% (n = 30), 30.6% (n = 37), 5.0% (n = 6), and 0.8% (n = 1) resistance, respectively. Compared to the resistance rates of other quinolones (LEV [36.4%, n = 44] and CIP [37.2%, n = 45]), sitafloxacin showed the best antibiotic performance against Helicobacter strains (0%, n = 0). Furthermore, sitafloxacin also inhibited the growth of 14 H. pylori strains (12.4%), which were resistant to both of clarithromycin, and metronidazole, and 27 strains (22.3%) with multidrug resistance. Conclusions: Sitafloxacin might be a new promising candidate for Helicobacter eradication where antibiotic resistance for Helicobacter is an emerging medical burden, such as in Korea.
Anthocyanins in Colorectal Cancer Prevention Review
Authors: Shi Ni , Chen Xiaoxin , Chen Tong
Published: October 2021

Colorectal cancer (CRC) is still a big health burden worldwide. Nutrition and dietary factors are known to affect colorectal cancer development and prognosis. The protective roles of diets rich in fruits and vegetables have been previously reported to contain high levels of cancer-fighting phytochemicals. Anthocyanins are the most abundant flavonoid compounds that are responsible for the bright colors of most blue, purple, and red fruits and vegetables, and have been shown to contribute to the protective effects of fruits and vegetables against cancer and other chronic diseases. Berries and grapes are the most common anthocyanin-rich fruits with antitumor effects. The antitumor effects of anthocyanins are determined by their structures and bioavailability as well as how they are metabolized. In this review, we aimed to discuss the preventive as well as therapeutic potentials of anthocyanins in CRC. We summarized the antitumor effects of anthocyanins and the mechanisms of action. We also discussed the potential pharmaceutical application of anthocyanins in practice.
Oxidative Stress Linked Organ Lipid Hydroperoxidation and Dysregulation in Mouse Model of Nonalcoholic Steatohepatitis: Revealed by Lipidomic Profiling of Liver and Kidney
Authors: Wu Yue , Chen Zhen , Fuda Hirotoshi , Tsukui Takayuki , Wu Xunzhi , Shen Nianqiu , Saito Natsuki , Chiba Hitoshi , Hui Shu-Ping
Published: October 2021

Nonalcoholic steatohepatitis (NASH) is a prevalent disease related to lipid metabolism disorder and oxidative stress. Lipid hydroperoxidation is known to be a critical driving force of various disorders and diseases. However, the combination of both intact and hydroperoxidized lipids in NASH has not yet been studied. In this work, the liver and kidney samples from NASH-model mice were comprehensively investigated by using the LC/MS-based lipidomic analysis. As a result, triglycerides showed the amount accumulation and the profile alteration for the intact lipids in the NASH group, while phosphatidylethanolamines, lysophosphatidylethanolamines, plasmalogens, and cardiolipins largely depleted, suggesting biomembrane damage and mitochondria dysfunction. Notably, the lipid hydroperoxide species of triglyceride and phosphatidylcholine exhibited a significant elevation in both the liver and the kidney of the NASH group and showed considerable diagnostic ability. Furthermore, the relationship was revealed between the lipid metabolism disturbance and the lipid hydroperoxide accumulation, which played a key role in the vicious circle of NASH. The present study suggested that the omics approach to the lipid hydroperoxide profile might be the potential diagnostic marker of NASH and other oxidative stress-related diseases, as well as the evaluative treatment index of antioxidants.
Optimization of Ultrasound-Assisted Extraction of Bioactive Compounds from Acacia Seyal Gum Using Response Surface Methodology and Their Chemical Content Identification by Raman, FTIR, and GC-TOFMS
Authors: Maher Tahani , Kabbashi Nassereldeen A. , Mirghani Mohamed E. S. , Alam Md Z. , Daddiouaissa Djabir , Abdulhafiz Ferid , Reduan Mohd Farhan Hanif , Omran Jihad I. , Abdul Razab Mohammad Khairul Azhar , Mohammed Arifullah
Published: October 2021

Acacia Seyal gum (ASG), also known as gum Arabic, is an antioxidant-rich soluble fiber. ASG has been reported to have many biological activities, including anticancer, antidiabetic, antiulcer, and immunomodulatory activity. Extraction of bioactive compounds from ASG is commonly performed using conventional extraction methods. However, these techniques have certain limitation in terms of extraction time, energy, and solvent requirements. Ultrasound-assisted extraction (UAE) could be used as an alternative technique to extract bioactive compounds in less time, at low temperature, and with less energy and solvent requirements. In this study, the UAE extraction of ASG was optimized using response surface methodology (RSM). A face-centered central composite design (FCCCD) was used to monitor the effect of different independent factors of ultrasound operation (sonication time, temperature, and solvent ratio) on ASG extraction yield. In addition, screening and characterization of phytochemicals in 60% ethanol ASG extract was carried out using Raman microscopy, Fourier transform infrared spectroscopy (FTIR), and gas chromatography time-of-flight mass spectroscopy (GC-TOFMS) analysis. The results indicated that, under optimal conditions (extraction time 45 min, extraction temperature 40 °C, and solid–liquid ratio of 1:25 g/mL), the yield of ASG was 75.87% ± 0.10. This yield was reasonably close to the predicted yield of 75.39% suggested by the design of experiment. The ANOVA revealed that the model was highly significant due to the low probability value (p < 0.0001). Raman spectrum fingerprint detected polysaccharides, such as galactose and glucose, and protein like lysine and proline, while FTIR spectrum revealed the presence of functional groups peaks value of alkanes, aldehydes, aliphatic amines, and phenol. GC-TOFMS spectroscopic detected the presence of strong d-galactopyranose, carotenoid, and lycopene antioxidant compounds. In conclusion, this study demonstrated that the UAE technique is an efficient method to achieve a high yield of ASG extracts. The selected model is adequate to optimize the extraction of several chemical compounds reported in this study.
Design of Multi-Receptive Field Fusion-Based Network for Surface Defect Inspection on Hot-Rolled Steel Strip Using Lightweight Dataset
Authors: Tang Wei-Peng , Liong Sze-Teng , Chen Chih-Cheng , Tsai Ming-Han , Hsieh Ping-Cheng , Tsai Yu-Ting , Chen Shih-Hsin , Wang Kun-Ching
Published: October 2021

With the advancement of industrial intelligence, defect recognition has become an indispensable part of facilitating surface quality in the steel manufacturing process. To assure product quality, most previous studies were typically trained with many defect samples. Nonetheless, a large quantity of defect samples is difficult to obtain, owing to the rare occurrence of defects. In general, deep learning-based methods underperformed as they have inherent limitations due to inadequate information, thereby restraining the application of models. In this study, a two-level Gaussian pyramid is applied to decompose raw data into different resolution levels simultaneously filtering the noises to acquire compact and representative features. Subsequently, a multi-receptive field fusion-based network (MRFFN) is developed to learn the hierarchical features and synthesize the respective prediction scores to form the final recognition result. As a result, the proposed method is capable of exhibiting an outstanding performance of 99.75% when trained using a lightweight dataset. In addition, the experiments conducted using the disturbance defect dataset showed the robustness of the proposed MRFFN against common noises and motion blur.
Automated Coastline Extraction Using the Very High Resolution WorldView (WV) Satellite Imagery and Developed Coastline Extraction Tool (CET)
Authors: Domazetović Fran , Šiljeg Ante , Marić Ivan , Faričić Josip , Vassilakis Emmanuel , Panđa Lovre
Published: October 2021

The accurate extraction of a coastline is necessary for various studies of coastal processes, as well as for the management and protection of coastal areas. Very high-resolution satellite imagery has great potential for coastline extraction; however, noises in spectral data can cause significant errors. Here, we present a newly developed Coastal Extraction Tool (CET) that overcomes such errors and allows accurate and time-efficient automated coastline extraction based on a combination of WorldView-2 (WV-2) multispectral imagery and stereo-pair-derived digital surface model (DSM). Coastline extraction is performed and tested on the Iž-Rava island group, situated within the Northern Dalmatian archipelago (Croatia). Extracted coastlines were compared to (a) coastlines extracted from state topographic map (1:25,000), and (b) coastline extracted by another available tool. The accuracy of the extracted coastline was validated with centimeter accuracy reference data acquired using a UAV system (Matrice 600 Pro + MicaSense RedEdge-MX). Within the study area, two small islets were detected that have not been mapped during the earlier coastline mapping efforts. CET proved to be a highly accurate coastline mapping technique that successfully overcomes spectral-induced errors. In future research, we are planning to integrate data obtained by UAVs infrared thermography (IRT) and in situ sensors, measuring sea and land surface temperatures (SST and LST), into the CET, given that this has shown promising results. Considering its accuracy and ease of use, we suggest that CET can be applied for automated coastline extraction in other large and indented coastal areas. Additionally, we suggest that CET could be applied in longitudinal geomorphological coastal erosion studies for the automated detection of spatio-temporal coastline displacement.
A Study on Soot and Ash Accumulation Characteristics of Diesel Particulate Filter Substrate Using Nondestructive Computed Radiography X-ray Imaging Technique
Authors: Park Giyoung , Ryu Hoyoung , Kwon Manjae , Lee Seangwock
Published: October 2021

The diesel particulate filter of a diesel engine is an after-treatment device that removes particulate matter from exhaust emissions by filtering and oxidating them through a regeneration process. When the diesel particulate filter is damaged, a vehicle inspection is usually performed; if the cause is not found through using on-board diagnostics, then the diesel particulate filter is removed, and a visual test is conducted. However, it is not easy to find the exact cause of the diesel particulate filter being damaged, and a visual test takes a long time as the diesel particulate filter substrate is covered by a canister. In this study, using the computed radiography X-ray imaging technique, X-ray images were taken after placing an accumulated amount of carbon powder, similar to soot and ash powder in the substrate. Results confirmed that carbon powder and ash powder were shown in white in X-ray images, leading to a conclusion that distinguishing between carbon powder and ash powder is possible by analyzing the pixel value through the image processing technique. However, since pixel values alone are insufficient for exact quantitative evaluation, various studies and analyses are necessary for quantitative evaluation.
Decellularized Skeletal Muscles Support the Generation of In Vitro Neuromuscular Tissue Models
Authors: Raffa Paolo , Easler Maria , Cecchinato Francesca , Auletta Beatrice , Scattolini Valentina , Perin Silvia , Gerli Mattia Francesco Maria , Caccin Paola , Elvassore Nicola , De Coppi Paolo , Urciuolo Anna
Published: October 2021

Decellularized skeletal muscle (dSkM) constructs have received much attention in recent years due to the versatility of their applications in vitro. In search of adequate in vitro models of the skeletal muscle tissue, the dSkM offers great advantages in terms of the preservation of native-tissue complexity, including three-dimensional organization, the presence of residual signaling molecules within the construct, and their myogenic and neurotrophic abilities. Here, we attempted to develop a 3D model of neuromuscular tissue. To do so, we repopulated rat dSkM with human primary myogenic cells along with murine fibroblasts and we coupled them with organotypic rat spinal cord samples. Such culture conditions not only maintained multiple cell type viability in a long-term experimental setup, but also resulted in functionally active construct capable of contraction. In addition, we have developed a customized culture system which enabled easy access, imaging, and analysis of in vitro engineered co-cultures. This work demonstrates the ability of dSkM to support the development of a contractile 3D in vitro model of neuromuscular tissue fit for long-term experimental evaluations.
An Improved Multiple Features and Machine Learning-Based Approach for Detecting Clickbait News on Social Networks
Authors: Al-Sarem Mohammed , Saeed Faisal , Al-Mekhlafi Zeyad Ghaleb , Mohammed Badiea Abdulkarem , Hadwan Mohammed , Al-Hadhrami Tawfik , Alshammari Mohammad T. , Alreshidi Abdulrahman , Alshammari Talal Sarheed
Published: October 2021

The widespread usage of social media has led to the increasing popularity of online advertisements, which have been accompanied by a disturbing spread of clickbait headlines. Clickbait dissatisfies users because the article content does not match their expectation. Detecting clickbait posts in online social networks is an important task to fight this issue. Clickbait posts use phrases that are mainly posted to attract a user’s attention in order to click onto a specific fake link/website. That means clickbait headlines utilize misleading titles, which could carry hidden important information from the target website. It is very difficult to recognize these clickbait headlines manually. Therefore, there is a need for an intelligent method to detect clickbait and fake advertisements on social networks. Several machine learning methods have been applied for this detection purpose. However, the obtained performance (accuracy) only reached 87% and still needs to be improved. In addition, most of the existing studies were conducted on English headlines and contents. Few studies focused specifically on detecting clickbait headlines in Arabic. Therefore, this study constructed the first Arabic clickbait headline news dataset and presents an improved multiple feature-based approach for detecting clickbait news on social networks in Arabic language. The proposed approach includes three main phases: data collection, data preparation, and machine learning model training and testing phases. The collected dataset included 54,893 Arabic news items from Twitter (after pre-processing). Among these news items, 23,981 were clickbait news (43.69%) and 30,912 were legitimate news (56.31%). This dataset was pre-processed and then the most important features were selected using the ANOVA F-test. Several machine learning (ML) methods were then applied with hyper-parameter tuning methods to ensure finding the optimal settings. Finally, the ML models were evaluated, and the overall performance is reported in this paper. The experimental results show that the Support Vector Machine (SVM) with the top 10% of ANOVA F-test features (user-based features (UFs) and content-based features (CFs)) obtained the best performance and achieved 92.16% of detection accuracy.
The Interaction between the LEO Satellite Constellation and the Space Debris Environment
Authors: Ren Shuyi , Yang Xiaohua , Wang Ronglan , Liu Siqing , Sun Xiaojing
Published: October 2021

The wide application of satellite constellations in the field of space-based global communications and remote sensing has led to a substantial increase in small-satellite launch plans, a sharp increase in the density of space objects in low-Earth orbit (LEO), and a reduction in available orbit and frequency resources. This will further aggravate the trend of deterioration of the space debris environment. Taking the Starlink constellation as an example, this paper describes the influence of the constellation from the environmental debris flux of the satellite, the evaluation of the number of evasion maneuvers, the change of risk level, the success rate of post mission disposal (PMD) and the growth rate of space objects. The simulation results show that the collision risk of the Starlink constellation is related to the orbital parameters, and the higher success rate of post-mission disposal (PMD) can reduce the collision risk of the constellation. The large constellations increases the growth rate of space objects, and even if all the satellites are disposed of after the mission, the impact of constellations on the space environment can not be offset.
Hardware-in-the-Loop Test of a Prosthetic Foot
Authors: Insam Christina , Ballat Lisa-Marie , Lorenz Felix , Rixen Daniel Jean
Published: October 2021

For a targeted development process of foot prostheses, a profound understanding of the dynamic interaction between humans and prostheses is necessary. In engineering, an often employed method to investigate the dynamics of mechanical systems is Hardware-in-the-Loop (HiL). This study conducted a fundamental investigation of whether HiL could be an applicable method to study the dynamics of an amputee wearing a prosthesis. For this purpose, a suitable HiL setup is presented and the first-ever HiL test of a prosthetic foot performed. In this setup, the prosthetic foot was tested on the test bench and coupled in real-time to a cosimulation of the amputee. The amputee was modeled based on the Virtual Pivot Point (VPP) model, and one stride was performed. The Center of Mass (CoM) trajectory, the Ground Reaction Forces (GRFs), and the hip torque were qualitatively analyzed. The results revealed that the basic gait characteristics of the VPP model can be replicated in the HiL test. Still, there were several limitations in the presented HiL setup, such as the limited actuator performance. The results implied that HiL may be a suitable method for testing foot prostheses. Future work will therefore investigate whether changes in the gait pattern can be observed by using different foot prostheses in the HiL test.
Data-Oriented Language Implementation of the Lattice–Boltzmann Method for Dense and Sparse Geometries
Authors: Tomczak Tadeusz
Published: October 2021

The performance of lattice–Boltzmann solver implementations usually depends mainly on memory access patterns. Achieving high performance requires then complex code which handles careful data placement and ordering of memory transactions. In this work, we analyse the performance of an implementation based on a new approach called the data-oriented language, which allows the combination of complex memory access patterns with simple source code. As a use case, we present and provide the source code of a solver for D2Q9 lattice and show its performance on GTX Titan Xp GPU for dense and sparse geometries up to 40962 nodes. The obtained results are promising, around 1000 lines of code allowed us to achieve performance in the range of 0.6 to 0.7 of maximum theoretical memory bandwidth (over 2.5 and 5.0 GLUPS for double and single precision, respectively) for meshes of sizes above 10242 nodes, which is close to the current state-of-the-art. However, we also observed relatively high and sometimes difficult to predict overheads, especially for sparse data structures. The additional issue was also a rather long compilation, which extended the time of short simulations, and a lack of access to low-level optimisation mechanisms.
Numerical Simulation of Enhanced Photoacoustic Generation and Wavefront Shaping by a Distributed Laser Array
Authors: Hou Ruijie , Xu Bin , Xia Zhiying , Zhang Yang , Liu Weiping , Glorieux Christ , Marsh John H. , Hou Lianping , Liu Xuefeng , Xiong Jichuan
Published: October 2021

In photoacoustic imaging, the use of arrayed laser sources brings several advantages. Acoustic waves can be generated with flexible control of wavefronts, bringing functionality such as ultrasonic beam steering and focusing. The use of arrays reduces the optical intensity while increasing the strength of the ultrasonic wave, bringing the advantages of improved signal-to-noise ratio (SNR) while avoiding laser-induced damage. In this paper, we report a numerical model for studying the generation and shaping of acoustic wavefronts with laser arrays. The propagation of mechanical waves, photoacoustically generated by thermal expansion, is simulated and discussed in detail. In addition, a partially delayed distributed array is studied both theoretically and quantitatively. The developed model for wavefront control through time-delayed laser pulses is shown to be highly suited for the optimization of laser array generation schemes.
Radio-Frequency Undulators, Cyclotron Auto Resonance Maser and Free Electron Lasers
Authors: Di Palma Emanuele , Ceccuzzi Silvio , Ravera Gian Luca , Sabia Elio , Spassovsky Ivan , Dattoli Giuseppe
Published: October 2021

We discuss a hybrid Free Electron Laser (FEL) architecture operating with a RF undulator provided by a powerful Cyclotron Auto-Resonance Maser (CARM). We outline the design elements to operate a compact X-ray device. We review the essential aspects of wave undulator FEL theory and of CARM devices.
User-Centered Design and Evaluation of an Upper Limb Rehabilitation System with a Virtual Environment
Authors: Ríos-Hernández Monserrat , Jacinto-Villegas Juan Manuel , Portillo-Rodríguez Otniel , Vilchis-González Adriana Herlinda
Published: October 2021

Virtual environments (VEs) and haptic devices increase patients’ motivation. Furthermore, they observe their performance during rehabilitation. However, some of these technologies present disadvantages because they do not consider therapists’ needs and experience. This research presents the development and usability evaluation of an upper limb rehabilitation system based on a user-centered design approach for patients with moderate or mild stroke that can perform active rehabilitation. The system consists of a virtual environment with four virtual scenarios and a developed haptic device with vibrotactile feedback, and it can be visualized using a monitor or a Head-Mounted Display (HMD). Two evaluations were carried out; in the first one, five therapists evaluated the system’s usability using a monitor through the System Usability Scale, the user experience with the AttrakDiff questionnaire, and the functionality with customized items. As a result of these tests, improvements were made to the system. The second evaluation was carried out by ten volunteers who evaluated the usability, user experience, and performance with a monitor and HMD. A comparison of the therapist and volunteer scores has shown an increase in the usability evaluation (from 78 to >85), the hedonic score rose from 0.6 to 2.23, the pragmatic qualities from 1.25 to 2.20, and the attractiveness from 1.3 to 2.95. Additionally, the haptic device and the VE showed no relevant difference between their performance when using a monitor or HMD. The results show that the proposed system has the characteristics to be a helpful tool for therapists and upper limb rehabilitation.
Hands-On Deformation of Volumetric Anatomical Images on a Touchscreen
Authors: Torres Rosell , Rodríguez Alejandro , Otaduy Miguel
Published: October 2021

In this work, we propose a novel metaphor to interact with volumetric anatomical images, e.g., magnetic resonance imaging or computed tomography scans. Beyond simple visual inspection, we empower users to reach the visible anatomical elements directly with their hands, and then move and deform them through natural gestures, while respecting the mechanical behavior of the underlying anatomy. This interaction metaphor relies on novel technical methods that address three major challenges: selection of anatomical elements in volumetric images, mapping of 2D manipulation gestures to 3D transformations, and real-time deformation of the volumetric images. All components of the interaction metaphor have been designed to capture the user’s intent in an intuitive manner, solving the mapping from the 2D touchscreen to the visible elements of the 3D volume. As a result, users have the ability to interact with medical volume images much like they would do with physical anatomy, directly with their hands.
The Role of Nutritional Habits and Moderate Red Wine Consumption in PON1 Status in Healthy Population
Authors: Navarro-García Fidel , Ponce-Ruíz Néstor , Rojas-García Aurora Elizabeth , Ávila-Villarreal Gabriela , Herrera-Moreno José Francisco , Barrón-Vivanco Briscia S. , Bernal-Hernández Yael Y. , González-Arias Cyndia Azucena , Medina-Díaz Irma Martha
Published: October 2021

Paraoxonase 1 (PON1) plays a role as antioxidant on HDL. Including in diet additionally ingest of polyphenolic compounds can stimulate PON1 transcription and increase its activity. The aim of this study was to evaluate the effect of dietary intake, red wine consumption, and PON1 genotypes (Q192R, L55M and C-108T) on the specific activity of PON1 in a healthy population. A descriptive and analytical pilot study was conducted in Mexican volunteers clinically healthy (n = 45) aged from 21–59 years. Over 6 weeks, the study participants ingested 120 mL of red wine per day. PON1 concentration, PON1 activities, genetic polymorphisms and dietary intake were evaluated. The preliminary fingerprinting of the wine was determined to corroborate the presence of phenolic compounds such as tannins and gallotannins. Neither dietary intake nor PON1 genotypes showed an effect on the specific activity of PON1. However, a significant increase in specific AREase activity after red wine consumption period was observed in the study participants. Our data suggest that the moderate consumption of red wine has a beneficial effect on PON1 specific AREase activity in this healthy Mexican population.
Gill Histopathology as a Biomarker for Discriminating Seasonal Variations in Water Quality
Authors: Marinović Zoran , Miljanović Branko , Urbányi Béla , Lujić Jelena
Published: October 2021

Histopathological alterations in various fish organs have a pronounced value in aquatic toxicology and are widely used in environmental monitoring. The aim of this study was to evaluate whether histopathological alterations in fish gills can discriminate seasonal variations in environmental conditions within the same aquatic ecosystem, and if so, which alterations contributed the most to seasonal differentiation. Microscopic examination of common bream Abramis brama gills displayed various alterations in gill structure, including epithelial hypertrophy, hyperplasia, mucous and chloride cell alterations, epithelial lifting, necrosis, hyperemia and aneurism. These alterations were subsequently quantified by a semi-quantitative analysis in order to detect differences in the intensity of the mentioned alterations. Epithelial hypertrophy, hyperplasia, epithelial lifting and necrosis varied significantly between seasons with only necrosis being significantly higher in the first season. Discriminant canonical analysis displayed that epithelial hyperplasia, mucous cell alterations, epithelial lifting and necrosis contributed the most to discrimination between seasons. Overall, this study demonstrates that histopathological biomarkers in fish gills can be used in discriminating seasonal variations in water quality within the same aquatic ecosystem.
A Review on Machine and Deep Learning for Semiconductor Defect Classification in Scanning Electron Microscope Images
Authors: López de la Rosa Francisco , Sánchez-Reolid Roberto , Gómez-Sirvent José L. , Morales Rafael , Fernández-Caballero Antonio
Published: October 2021

Continued advances in machine learning (ML) and deep learning (DL) present new opportunities for use in a wide range of applications. One prominent application of these technologies is defect detection and classification in the manufacturing industry in order to minimise costs and ensure customer satisfaction. Specifically, this scoping review focuses on inspection operations in the semiconductor manufacturing industry where different ML and DL techniques and configurations have been used for defect detection and classification. Inspection operations have traditionally been carried out by specialised personnel in charge of visually judging the images obtained with a scanning electron microscope (SEM). This scoping review focuses on inspection operations in the semiconductor manufacturing industry where different ML and DL methods have been used to detect and classify defects in SEM images. We also include the performance results of the different techniques and configurations described in the articles found. A thorough comparison of these results will help us to find the best solutions for future research related to the subject.
Unsteady Flow Characteristics of an Oscillating Piezoelectric Fan Blade at High Reynolds Numbers
Authors: Chen Yiyang , Li Jianxin , Wang Ziwen , Yan Yan , Cui Jiahuan
Published: October 2021

Piezoelectric fans have started to play an essential role in small-scale heat removal applications in recent years due to their high reliability and efficiency. In this study, an experimental study on the flow field characteristics produced by an oscillating piezoelectric fan at various Reynolds numbers (140 < Re < 550) in a quiescent air environment is investigated. Time resolved particle image velocimetry (PIV) measurements are performed for the flow field visualization. The flow pattern generated by the oscillating fan blade in the longitudinal plane changes as the Reynolds number increases. The ratio between the trailing edge velocity and side edge velocity increases as the Reynolds number increases. As a result, the trailing edge plays a more important role in driving fluid at a higher Reynolds number. Multiple vortexes are shed from the trailing edge during one oscillation cycle and is observed only at a high Reynolds number. This vortex shedding increases the unsteadiness of velocity field significantly, resulting in a turbulence intensity level beyond 100%. This result implies that turbulence models used in numerical studies need to be carefully validated as some might struggle at this highly turbulent flow regime.
The Effects of Aerodynamic Interference on the Aerodynamic Characteristics of a Twin-Box Girder
Authors: Wu Buchen , Xue Geng , Feng Jie , Laima Shujin
Published: October 2021

To investigate the aerodynamic characteristics of a twin-box girder in turbulent incoming flow, we carried out wind tunnel tests, including two aerodynamic interferences: leading body-height grid, and leading circular cylinder. In this study, the pressure distribution and the mean and fluctuating aerodynamic forces with the two interferences are compared with bare deck in detail to investigate the relationship between aerodynamic characteristics and the incoming flow characteristics (including Reynolds number and turbulence intensity). The experimental results reveal that, owing to the body-height flow characteristics around the deck interfered with by the body-height grid, the disturbed aerodynamic characteristics of the twin-box girder differ considerably from those of the bare twin-box girder. At the upstream girder, due to the vortex emerging from the body-height grid breaking the separation bubble, pressure plateaus in the upper and lower surface are eliminated. In addition, the turbulence generated by the body-height grid reduces the Reynolds number sensitivity of the twin-box girder. At a relatively high Reynolds number, the fluctuating forces are mainly dominated by turbulence intensity, and the time-averaged forces show almost no change under high turbulence intensity. At a low Reynolds number, the time-averaged forces change significantly with the turbulence intensity. Moreover, at a low Reynolds number, the wake of the leading cylinder effectively forces the boundary layer to transition to turbulence, which reduces the Reynolds number sensitivity of the mean aerodynamic forces and breaks the separation bubbles. Additionally, the fluctuating drag force and the fluctuating lift force are insensitive to the diameter and the spacing ratio.