bluuebunny/crossref_metadata_2025 huggingface dataset:
Transmembrane Mucin Response in Conjunctival Epithelial Cells Exposed to Wall Shear Stresses
Authors: Itah Shir , Elad David , Jaffa Ariel J. , Grisaru Dan , Rosner Mordechai
Published: April 2023

Human conjunctival epithelium cells (HCEC) line the inner surface of the eyelid and cover the sclera and are continuously subjected to wall shear stresses (WSS). The effects of external forces on the conjunctival epithelium are not fully known. The conjunctival epithelium contains stratified squamous cells that synthesize the membrane-spanning mucins MUC1 and MUC16, which play important roles in protecting the ocular surface. Alterations in both gel-forming and membrane-tethered mucins occur in drying ocular surface diseases. The aim of this study was to explore the mechanobiological characteristics of transmembrane mucin secretion and cellular alterations of primary HCEC exposed to airflow-induced WSS perturbations. We exposed the HCEC to a steady WSS of 0.5 dyne/cm2 for durations of 15 and 30 min. Cytoskeletal alterations and MUC1 secretions were studied using immunohistochemically fluorescent staining with specific antibodies. We investigated for the first time an in vitro model of membrane-tethered mucin secretion by HCEC in response to WSS. The exposure of HCEC to WSS increased the polymerization of F-actin, altered the cytoskeletal shape and reduced the secretion of membrane-tethered MUC1.
Clinicopathological Features and Survival Analysis in Molecular Subtypes of Muscle-Invasive Bladder Cancer
Authors: Sanguedolce Francesca , Falagario Ugo Giovanni , Zanelli Magda , Palicelli Andrea , Zizzo Maurizio , Ascani Stefano , Tortorella Simona , Mancini Vito , Cormio Angelo , Carrieri Giuseppe , Cormio Luigi
Published: April 2023

Molecular subtyping of bladder cancer (BC) aims to capture the biological heterogeneity of this complex disease in order to provide better patient risk stratification. Immunohistochemical (IHC) markers are regarded as promising surrogates to classify BCs into luminal and basal subtypes in routine practice. We investigated the correlation between the molecular subclassification, assessed through IHC, and the conventional prognostic variables of a cohort of 93 muscle-invasive BCs (MIBCs), with a focus on the pattern of muscularis propria (MP) invasion, and evaluated their association with outcome. Basal, luminal, double-positive (DP), and double-negative (DN) phenotypes were identified according to the coordinate expression of 1 basal (CK5/6) and 2 luminal (CK20, GATA3) markers, and accounted for 33.3%, 32.3%, 3.2%, and 31.2% (Scheme #1) and 9.7%, 60.2%, 26.9%, and 3.2% (Scheme #2). There was a significant association between the pattern of MP invasion and the molecular subtypes according to Scheme #2, in that all 8 basal and DN cases, as well as 83% of DP cases, had a non-infiltrative invasion pattern. No consistent differences were observed in terms of OS and CSS between the molecular subtypes obtained through surrogate IHC markers. In keeping with previous studies, we report the correlation between the identification of BC subtypes and the presence of morphological prognostic factors, supporting the need for a comprehensive pathological evaluation, including clinicopathological and molecular parameters, in order to improve the diagnosis and management of MIBC.
A Lipidated Single-B-Chain Derivative of Relaxin Exhibits Improved In Vitro Serum Stability without Altering Activity
Authors: Praveen Praveen , Wang Chao , Handley Thomas N. G. , Wu Hongkang , Samuel Chrishan S. , Bathgate Ross A. D. , Hossain Mohammed Akhter
Published: April 2023

Human relaxin-2 (H2 relaxin) is therapeutically very important due to its strong anti-fibrotic, vasodilatory, and cardioprotective effects. Therefore, relaxin’s receptor, relaxin family peptide receptor 1 (RXFP1), is a potential target for the treatment of fibrosis and related disorders, including heart failure. H2 relaxin has a complex two-chain structure (A and B) and three disulfide bridges. Our laboratory has recently developed B7-33 peptide, a single-chain agonist based on the B-chain of H2 relaxin. However, the peptide B7-33 has a short circulation time in vitro in serum (t1/2 = ~6 min). In this study, we report structure-activity relationship studies on B7-33 utilizing different fatty-acid conjugations at different positions. We have shown that by fatty-acid conjugation with an appropriate spacer length, the in vitro half-life of B7-33 can be increased from 6 min to 60 min. In the future, the lead lipidated molecule will be studied in animal models to measure its PK/PD properties, which will lead to their pre-clinical applications.
Pathophysiological Involvement of Mast Cells and the Lipid Mediators in Pulmonary Vascular Remodeling
Authors: Moriyama Hidenori , Endo Jin
Published: April 2023

Mast cells are responsible for IgE-dependent allergic responses, but they also produce various bioactive mediators and contribute to the pathogenesis of various cardiovascular diseases, including pulmonary hypertension (PH). The importance of lipid mediators in the pathogenesis of PH has become evident in recent years, as exemplified by prostaglandin I2, the most central therapeutic target in pulmonary arterial hypertension. New bioactive lipids other than eicosanoids have also been identified that are associated with the pathogenesis of PH. However, it remains largely unknown how mast cell-derived lipid mediators are involved in pulmonary vascular remodeling. Recently, it has been demonstrated that mast cells produce epoxidized n-3 fatty acid (n-3 epoxides) in a degranulation-independent manner, and that n-3 epoxides produced by mast cells regulate the abnormal activation of pulmonary fibroblasts and suppress the progression of pulmonary vascular remodeling. This review summarizes the role of mast cells and bioactive lipids in the pathogenesis of PH. In addition, we introduce the pathophysiological role and therapeutic potential of n-3 epoxides, a mast cell-derived novel lipid mediator, in the pulmonary vascular remodeling in PH. Further knowledge of mast cells and lipid mediators is expected to lead to the development of innovative therapies targeting pulmonary vascular remodeling.
Genome-Wide Analysis of the Odorant Receptor Gene Family in Solenopsis invicta, Ooceraea biroi, and Monomorium pharaonis (Hymenoptera: Formicidae)
Authors: Zhang Bo , Yang Rong-Rong , Jiang Xing-Chuan , Xu Xiao-Xia , Wang Bing , Wang Gui-Rong
Published: April 2023

Olfactory systems in eusocial insects play a vital role in the discrimination of various chemical cues. Odorant receptors (ORs) are critical for odorant detection, and this family has undergone extensive expansion in ants. In this study, we re-annotated the OR genes from the most destructive invasive ant species Solenopsis invicta and 2 other Formicidae species, Ooceraea biroi and Monomorium pharaonis, with the aim of systematically comparing and analyzing the evolution and the functions of the ORs in ant species, identifying 356, 298, and 306 potential functional ORs, respectively. The evolutionary analysis of these ORs showed that ants had undergone chromosomal rearrangements and that tandem duplication may be the main contributor to the expansion of the OR gene family in S. invicta. Our further analysis revealed that 9-exon ORs had biased chromosome localization patterns in all three ant species and that a 9-exon OR cluster (SinvOR4–8) in S. invicta was under strong positive selection (Ka/Ks = 1.32). Moreover, we identified 5 S. invicta OR genes, namely SinvOR89, SinvOR102, SinvOR352, SinvOR327, and SinvOR135, with high sequence similarity (>70%) to the orthologs in O. biroi and M. pharaonis. An RT-PCR analysis was used to verify the antennal expression levels of these ORs, which showed caste-specific expression. The subsequent analysis of the antennal expression profiles of the ORs of the S. invicta workers from the polygyne and monogyne social forms indicated that SinvOR35 and SinvOR252 were expressed at much higher levels in the monogyne workers than in the polygyne workers and that SinvOR21 was expressed at higher levels in polygyne workers. Our study has contributed to the identification and analysis of the OR gene family in ants and expanded the understanding of the evolution and functions of the ORs in Formicidae species.
Establishment of an Efficient Agrobacterium-Mediated Genetic Transformation System to Enhance the Tolerance of the Paraquat Stress in Engineering Goosegrass (Eleusine Indica L.)
Authors: Luo Qiyu , Chen Shu , Nian Hai , Ma Qibing , Ding Yuyao , Hao Qinwen , Wei Jiping , Patel Jinesh D. , McElroy Joseph Scott , Liu Yaoguang , Chen Yong
Published: April 2023

Eleusine indica (goosegrass) is a problematic weed worldwide known for its multi-herbicide tolerance/resistance biotype. However, a genetic transformation method in goosegrass has not been successfully established, making a bottleneck for functional genomics studies in this species. Here, we report a successful Agrobacterium-mediated transformation method for goosegrass. Firstly, we optimized conditions for breaking seed dormancy and increasing seed germination rate. A higher callus induction rate from germinated seeds was obtained in N6 than in MS or B5 medium. Then the optimal transformation efficiency of the gus reporter gene was obtained by infection with Agrobacterium tumefaciens culture of OD600 = 0.5 for 30 min, followed by 3 days of co-cultivation with 300 μmol/L acetosyringone. Concentrations of 20 mg L−1 kanamycin and 100 mg L−1 timentin were used to select the transformed calli. The optimal rate of regeneration of the calli was generated by using 0.50 mg L−1 6-BA and 0.50 mg L−1 KT in the culture medium. Then, using this transformation method, we overexpressed the paraquat-resistant EiKCS gene into a paraquat-susceptible goosegrass biotype MZ04 and confirmed the stable inheritance of paraquat-resistance in the transgenic goosegrass lines. This approach may provide a potential mechanism for the evolution of paraquat-resistant goosegrass and a promising gene for the manipulation of paraquat-resistance plants. This study is novel and valuable in future research using similar methods for herbicide resistance.
The Anticancer Drug Daunomycin Directly Affects Gene Expression and DNA Structure
Authors: Nishio Takashi , Shimada Yohji , Yoshikawa Yuko , Kenmotsu Takahiro , Schiessel Helmut , Yoshikawa Kenichi
Published: April 2023

Daunomycin (DM), an anthracycline antibiotic, is frequently used to treat various cancers, but the direct effects of DM on gene expression and DNA structure are unclear. We used an in vitro cell-free system, optimized with spermine (SP), to study the effect of DM on gene expression. A bimodal effect of DM on gene expression, weak promotion followed by inhibition, was observed with increasing concentration of DM. We also performed atomic force microscopy observation to measure how DM affects the higher-order structure of DNA induced with SP. DM destroyed SP-induced flower-like conformations of DNA by generating double-strand breaks, and this destructive conformational change of DNA corresponded to the inhibitory effect on gene expression. Interestingly, the weakly enhanced cell-free gene expression occurred as DNA conformations were elongated or relaxed at lower DM concentrations. We expect these newly unveiled DM effects on gene expression and the higher-order structure of DNA will contribute further to the development and refinement of useful anticancer therapy chemicals.
Colorimetric Detection of Acenaphthene and Naphthalene Using Functionalized Gold Nanoparticles
Authors: Chuang Kai-Jen , Dong Meng-Ru , Laishram Purnima , Hong Gui-Bing
Published: April 2023

Polycyclic aromatic hydrocarbons are a class of chemicals that occur naturally. They generally demonstrate a high degree of critical toxicity towards humans. Acenaphthene and naphthalene contain compounds that are commonly found in the environment as compared to other PAHs. Consequently, a reliable method of detecting PAHs is crucial for the monitoring of water quality. A colorimetric method based on sodium nitrite-functionalized gold nanoparticles was developed in this study for acenaphthene and naphthalene detection. Different functionalized parameters are determined for the optimization of assay conditions. A linear relationship was found in the analyte concentration range of 0.1–10 ppm with the limit of detection for acenaphthene and naphthalene being 0.046 ppm and 0.0015 ppm, respectively, under the optimized assay conditions. The method’s recovery rate for actual samples falls within the range of 98.4–103.0%. In selective and anti-interference tests, the presence of cations and anions has minimal impact on the detection of the analyte. The colorimetric detection method proposed in this study effectively determines the presence of the analyte in real water samples and has a high recovery rate.
Coarse-Grained Molecular Simulations and Ensemble-Based Mutational Profiling of Protein Stability in the Different Functional Forms of the SARS-CoV-2 Spike Trimers: Balancing Stability and Adaptability in BA.1, BA.2 and BA.2.75 Variants
Authors: Verkhivker Gennady , Alshahrani Mohammed , Gupta Grace
Published: April 2023

Evolutionary and functional studies have suggested that the emergence of Omicron variants can be determined by multiple fitness tradeoffs including immune escape, binding affinity, conformational plasticity, protein stability, and allosteric modulation. In this study, we embarked on a systematic comparative analysis of the conformational dynamics, electrostatics, protein stability, and allostery in the different functional states of spike trimers for BA.1, BA.2, and BA.2.75 variants. Using efficient and accurate coarse-grained simulations and atomistic reconstruction of the ensembles, we examined the conformational dynamics of the spike trimers that agree with the recent functional studies, suggesting that BA.2.75 trimers are the most stable among these variants. A systematic mutational scanning of the inter-protomer interfaces in the spike trimers revealed a group of conserved structural stability hotspots that play a key role in the modulation of functional dynamics and are also involved in the inter-protomer couplings through local contacts and interaction networks with the Omicron mutational sites. The results of mutational scanning provided evidence that BA.2.75 trimers are more stable than BA.2 and comparable in stability to the BA.1 variant. Using dynamic network modeling of the S Omicron BA.1, BA.2, and BA.2.75 trimers, we showed that the key network mediators of allosteric interactions are associated with the major stability hotspots that are interconnected along potential communication pathways. The network analysis of the BA.1, BA.2, and BA.2.75 trimers suggested that the increased thermodynamic stability of the BA.2.75 variant may be linked with the organization and modularity of the residue interaction network that allows for allosteric communications between structural stability hotspots and Omicron mutational sites. This study provided a plausible rationale for a mechanism in which Omicron mutations may evolve by targeting vulnerable sites of conformational adaptability to elicit immune escape while maintaining their control on balancing protein stability and functional fitness through robust allosteric communications with the stability hotspots.
Multi-Region Microdialysis Imaging Platform Revealed Dorsal Raphe Nucleus Calcium Signaling and Serotonin Dynamics during Nociceptive Pain
Authors: Akbar Latiful , Castillo Virgil Christian Garcia , Olorocisimo Joshua Philippe , Ohta Yasumi , Kawahara Mamiko , Takehara Hironari , Haruta Makito , Tashiro Hiroyuki , Sasagawa Kiyotaka , Ohsawa Masahiro , Akay Yasemin M. , Akay Metin , Ohta Jun
Published: April 2023

In this research, we combined our ultralight micro-imaging device for calcium imaging with microdialysis to simultaneously visualize neural activity in the dorsal raphe nucleus (DRN) and measure serotonin release in the central nucleus of the amygdala (CeA) and the anterior cingulate cortex (ACC). Using this platform, we observed brain activity following nociception induced by formalin injection in the mouse’s hind paw. Our device showed that DRN fluorescence intensity increased after formalin injection, and the increase was highly correlated with the elevation in serotonin release in both the CeA and ACC. The increase in calcium fluorescence intensity occurred during the acute and inflammatory phases, which suggests the biphasic response of nociceptive pain. Furthermore, we found that the increase in fluorescence intensity was positively correlated with mouse licking behavior. Lastly, we compared the laterality of pain stimulation and found that DRN fluorescence activity was higher for contralateral stimulation. Microdialysis showed that CeA serotonin concentration increased only after contralateral stimulation, while ACC serotonin release responded bilaterally. In conclusion, our study not only revealed the inter-regional serotonergic connection among the DRN, the CeA, and the ACC, but also demonstrated that our device is feasible for multi-site implantation in conjunction with a microdialysis system, allowing the simultaneous multi-modal observation of different regions in the brain.
Mineral Metabolism in Children: Interrelation between Vitamin D and FGF23
Authors: Pons-Belda Oscar D. , Alonso-Álvarez Mª Agustina , González-Rodríguez Juan David , Mantecón-Fernández Laura , Santos-Rodríguez Fernando
Published: April 2023

Fibroblast growth factor 23 (FGF23) was identified at the turn of the century as the long-sought circulating phosphatonin in human pathology. Since then, several clinical and experimental studies have investigated the metabolism of FGF23 and revealed its relevant pathogenic role in various diseases. Most of these studies have been performed in adult individuals. However, the mineral metabolism of the child is, to a large extent, different from that of the adult because, in addition to bone remodeling, the child undergoes a specific process of endochondral ossification responsible for adequate mineralization of long bones’ metaphysis and growth in height. Vitamin D metabolism is known to be deeply involved in these processes. FGF23 might have an influence on bones’ growth as well as on the high and age-dependent serum phosphate concentrations found in infancy and childhood. However, the interaction between FGF23 and vitamin D in children is largely unknown. Thus, this review focuses on the following aspects of FGF23 metabolism in the pediatric age: circulating concentrations’ reference values, as well as those of other major variables involved in mineral homeostasis, and the relationship with vitamin D metabolism in the neonatal period, in vitamin D deficiency, in chronic kidney disease (CKD) and in hypophosphatemic disorders.
SEMKIS-DSL: A Domain-Specific Language to Support Requirements Engineering of Datasets and Neural Network Recognition
Authors: Jahić Benjamin , Guelfi Nicolas , Ries Benoît
Published: April 2023

Neural network (NN) components are being increasingly incorporated into software systems. Neural network properties are determined by their architecture, as well as the training and testing datasets used. The engineering of datasets and neural networks is a challenging task that requires methods and tools to satisfy customers’ expectations. The lack of tools that support requirements specification languages makes it difficult for engineers to describe dataset and neural network recognition skill requirements. Existing approaches often rely on traditional ad hoc approaches, without precise requirement specifications for data selection criteria, to build these datasets. Moreover, these approaches do not focus on the requirements of the neural network’s expected recognition skills. We aim to overcome this issue by defining a domain-specific language that precisely specifies dataset requirements and expected recognition skills after training for an NN-based system. In this paper, we present a textual domain-specific language (DSL) called SEMKIS-DSL (Software Engineering Methodology for the Knowledge management of Intelligent Systems) that is designed to support software engineers in specifying the requirements and recognition skills of neural networks. This DSL is proposed in the context of our general SEMKIS development process for neural network engineering. We illustrate the DSL’s concepts using a running example that focuses on the recognition of handwritten digits. We show some requirements and recognition skills specifications and demonstrate how our DSL improves neural network recognition skills.
A Systematic Review of Effective Hardware and Software Factors Affecting High-Throughput Plant Phenotyping
Authors: Solimani Firozeh , Cardellicchio Angelo , Nitti Massimiliano , Lako Alfred , Dimauro Giovanni , Renò Vito
Published: April 2023

Plant phenotyping studies the complex characteristics of plants, with the aim of evaluating and assessing their condition and finding better exemplars. Recently, a new branch emerged in the phenotyping field, namely, high-throughput phenotyping (HTP). Specifically, HTP exploits modern data sampling techniques to gather a high amount of data that can be used to improve the effectiveness of phenotyping. Hence, HTP combines the knowledge derived from the phenotyping domain with computer science, engineering, and data analysis techniques. In this scenario, machine learning (ML) and deep learning (DL) algorithms have been successfully integrated with noninvasive imaging techniques, playing a key role in automation, standardization, and quantitative data analysis. This study aims to systematically review two main areas of interest for HTP: hardware and software. For each of these areas, two influential factors were identified: for hardware, platforms and sensing equipment were analyzed; for software, the focus was on algorithms and new trends. The study was conducted following the PRISMA protocol, which allowed the refinement of the research on a wide selection of papers by extracting a meaningful dataset of 32 articles of interest. The analysis highlighted the diffusion of ground platforms, which were used in about 47% of reviewed methods, and RGB sensors, mainly due to their competitive costs, high compatibility, and versatility. Furthermore, DL-based algorithms accounted for the larger share (about 69%) of reviewed approaches, mainly due to their effectiveness and the focus posed by the scientific community over the last few years. Future research will focus on improving DL models to better handle hardware-generated data. The final aim is to create integrated, user-friendly, and scalable tools that can be directly deployed and used on the field to improve the overall crop yield.
Is It Worth the Effort? Considerations on Text Mining in AI-Based Corporate Failure Prediction
Authors: Nießner Tobias , Nießner Stefan , Schumann Matthias
Published: April 2023

How can useful information extracted from unstructured data be used to contribute to a better prediction of corporate failure or bankruptcy? In this research, we examine a data set of 2,163,147 financial statements of German companies that are triple classified, i.e., solvent, financially distressed, and bankrupt. By classifying text features in terms of granularity and linguistic level of analysis, we show results for the potentials and limitations of approaches developed in this way. This study gives a first approach to evaluate and classify the likelihood of success of text mining approaches for extracting features that enhance the training database of AI-based solutions and improve corporate failure prediction models developed in this way. Our results are an indication that the adaptation of additional information sources for the financial evaluation of companies is indeed worthwhile, but approaches adapted to the context should be used instead of unspecific general text mining approaches.
Rational Design of Fullerene Derivatives for Improved Stability of p-i-n Perovskite Solar Cells
Authors: Ozerova Victoria V. , Mumyatov Alexander V. , Goryachev Andrey E. , Khakina Ekaterina A. , Peregudov Alexander S. , Aldoshin Sergey M. , Troshin Pavel A.
Published: April 2023

Perovskite solar cells (PSCs) with p-i-n architecture attracted particular attention from the research community due to their simple and scalable fabrication at low temperatures. However, the operational stability of p-i-n PSCs has to be improved, which requires the development of advanced charge transport interlayers. Fullerene derivatives such as phenyl-C61-butyric acid methyl ester (PC61BM) are commonly used as electron transport layer (ETL) materials in PSCs, though they strongly compromise the device stability. Indeed, it has been shown that PC61BM films actively absorb volatile products resulting from photodegradation of lead halide perovskites and transport them towards top metal electrode. Thus, there is an urgent need for development of new fullerene-based electron transport materials with improved properties, in particular the ability to heal defects on the perovskite films surface and block the diffusion of volatile perovskite photodegradation products. To address this challenge, a systematic variation of organic addends structure should be performed in order to tailor the properties of fullerene derivatives. Herein, we rationally designed a series of fullerene derivatives with different side chains and explored their performance as ETL materials in perovskite solar cells. It has been shown that among all studied compounds, a methanofullerene with thiophene pendant group enables both high efficiency and improved device operational stability. The obtained results suggest that further engineering of fullerene-based materials could pave a way for the development of advanced ETL materials enabling long lifetimes of p-i-n perovskite solar cells.
Cardiovascular Implantable Electronic Devices Enabled Remote Heart Failure Monitoring; What We Have Learned and Where to Go Next
Authors: Assa Solmaz , Vernooy Kevin , van Stipdonk Antonius M. W.
Published: April 2023

Despite recent developments, heart failure (HF) remains to be a great burden to the individual patient, entailing major morbidity and mortality. Moreover, HF is a great burden to overall healthcare, mainly because of frequent hospitalizations. Timely diagnosis of HF deterioration and implementation of appropriate therapy may prevent hospitalization and eventually improve a patient’s prognosis; however, depending on the patient’s presentation, the signs and symptoms of HF often offer too little therapeutic window to prevent hospitalizations. Cardiovascular implantable electronic devices (CIEDs) can provide real-time physiologic parameters and remote monitoring of these parameters can potentially help to identify patients at high risk. However, routine implementation of remote monitoring of CIEDs has still not been widely used in daily patient care. This review gives a detailed description of available metrics for remote HF monitoring, the studies that provide evidence of their efficacy, ways to implement them in clinical HF practice, as well as lessons learned on where to go on from where we currently are.
Why Don’t More Mitochondrial Diseases Exhibit Cardiomyopathy?
Authors: Singh Nina , Ren Mindong , Phoon Colin K. L.
Published: April 2023

Background: Although the heart requires abundant energy, only 20–40% of children with mitochondrial diseases have cardiomyopathies. Methods: We looked for differences in genes underlying mitochondrial diseases that do versus do not cause cardiomyopathy using the comprehensive Mitochondrial Disease Genes Compendium. Mining additional online resources, we further investigated possible energy deficits caused by non-oxidative phosphorylation (OXPHOS) genes associated with cardiomyopathy, probed the number of amino acids and protein interactors as surrogates for OXPHOS protein cardiac “importance”, and identified mouse models for mitochondrial genes. Results: A total of 107/241 (44%) mitochondrial genes was associated with cardiomyopathy; the highest proportion were OXPHOS genes (46%). OXPHOS (p = 0.001) and fatty acid oxidation (p = 0.009) defects were significantly associated with cardiomyopathy. Notably, 39/58 (67%) non-OXPHOS genes associated with cardiomyopathy were linked to defects in aerobic respiration. Larger OXPHOS proteins were associated with cardiomyopathy (p < 0.05). Mouse models exhibiting cardiomyopathy were found for 52/241 mitochondrial genes, shedding additional insights into biological mechanisms. Conclusions: While energy generation is strongly associated with cardiomyopathy in mitochondrial diseases, many energy generation defects are not linked to cardiomyopathy. The inconsistent link between mitochondrial disease and cardiomyopathy is likely to be multifactorial and includes tissue-specific expression, incomplete clinical data, and genetic background differences.
Quantitative and Qualitative Assessments of Retinal Structure with Variable A-Scan Rate Spectralis OCT: Insights into IPL Multilaminarity
Authors: Lupidi Marco , Mangoni Lorenzo , Centini Chiara , Pompucci Gregorio , Lanzafame Luca , Danieli Luca , Fruttini Daniela , Peiretti Enrico , Chhablani Jay , Mariotti Cesare
Published: April 2023

The aim of this study was to evaluate the qualitative and quantitative differences between 20 and 85 kHz A-scan rate optical coherence tomography (OCT) images acquired by spectral domain OCT. The study included 60 healthy subjects analyzed with horizontal linear scans with a variable A-scan rate (SHIFT technology, Heidelberg Engineering, Heidelberg, Germany). The retinal thickness measurement of each retinal layer was performed in three different positions (subfoveal, nasal, and temporal). The qualitative assessment was performed by two independent observers who rated every image with a score ranging from 1 (“sufficient”) to 3 (“excellent”) on the basis of three parameters: visualization of the vitreo-retinal interface, characterization of the retinal layers, and visualization of the sclero-choroidal interface. No statistically significant differences in terms of retinal layer thickness between the two A-scan rate scans were observed (p > 0.05). The coefficient of variation of the retinal thickness values was lower in the 20 kHz group (25.8% versus 30.1% with the 85 kHz). The 20 kHz images showed a higher quality index for both observers. An inner plexiform layer (IPL) multilaminarity was detected in 78.3% of patients from the 20 kHz group and in 40% of patients from the 85 kHz group (p < 0.05).
Mandible Angle Resection with the Retroauricular Approach
Authors: Lee Yoon Joo , Park Yunsung , Ha Yooseok , Kim Sunje
Published: April 2023

Square-shaped and large moon-shaped faces are commonly observed in Asians, and the contour of the mandible is associated with the shape of the lower part of the face. Mandible contouring surgery is performed to create a softer impression for East Asians. Currently, most surgeries are performed using an intraoral approach. External approaches have not been cosmetically attempted because of possible damage to the facial nerve and visible scarring and have been limited to mandible bone fracture reduction. This study included 42 patients who underwent mandibular angle reduction via classical intraoral incision and retroauricular incision between April 2019 and October 2021. Clinical outcomes were assessed using the Global Aesthetic Improvement Scale and Visual Analog Scale. Surgery was successful in all cases, with no significant complications. An appropriate mandibular contour was achieved postoperatively. All patients were satisfied with the outcome. Some patients experienced short-term complications, such as hematoma and wound disruption of the skin above the incision line. However, these improved within 3 weeks, and no serious long-term complications were observed. Mandible angle resection with the retroauricular approach is a promising alternative for patients, allowing speedy recovery and the resumption of routine daily life.
Early Recognition and Risk Stratification in Cardiogenic Shock: Well Begun Is Half Done
Authors: Polyzogopoulou Effie , Bezati Sofia , Karamasis Grigoris , Boultadakis Antonios , Parissis John
Published: April 2023

Cardiogenic shock is a complex syndrome manifesting with distinct phenotypes depending on the severity of the primary cardiac insult and the underlying status. As long as therapeutic interventions fail to divert its unopposed rapid evolution, poor outcomes will continue challenging health care systems. Thus, early recognition in the emergency setting is a priority, in order to avoid delays in appropriate management and to ensure immediate initial stabilization. Since advanced therapeutic strategies and specialized shock centers may provide beneficial support, it seems that directing patients towards the recently described shock network may improve survival rates. A multidisciplinary approach strategy commands the interconnections between the strategic role of the ED in affiliation with cardiac shock centers. This review outlines critical features of early recognition and initial therapeutic management, as well as the utility of diagnostic tools and risk stratification models regarding the facilitation of patient trajectories through the shock network. Further, it proposes the implementation of precise criteria for shock team activation and the establishment of definite exclusion criteria for streaming the right patient to the right place at the right time.
Short-Term Results of Operative Treatment of Primary Ileocecal Crohn’s Disease: Retrospective, Comparative Analysis between Early (Luminal) and Complicated Disease
Authors: Avellaneda Nicolas , Haug Tora , Worm Ørntoft Mai-Britt , Harsløf Sanne , Skovgaard Larsen Lars Peter , Tøttrup Anders
Published: April 2023

Early surgical treatment for patients with ileocecal Crohn’s disease (CD) could be an alternative to biological therapy. The aim of this study is to compare operative outcomes following ileocecal resection for patients with luminal and complicated CD. Patients operated for primary ileocecal CD during 8 years in one tertiary-referral hospital were allocated into 2 groups: those operated for early (luminal) disease (ECD), and for complications of CD (CCD). A retrospective comparative analysis was performed. A total of 273 patients were included in the analysis, 85 (31%) of which were in the ECD group. No difference was found regarding time from diagnosis to surgery. Surgical procedures were longer in the CCD group, with lower rates of laparoscopic approach (93 vs. 99%, p = 0.035) and higher conversion rates (20 vs. 2%, p < 0.001). ECD had non-significant differences in terms of major postoperative complications (9.4 vs. 14.9%, p = 0.215), shorter hospital stays, and lower rates of anastomotic leakage (3.5 vs. 6.8%, p = 0.285). Conversely, the CCD group had higher reoperation and re-hospitalization rates. Adequate timing for the indication of surgery in primary ileocecal CD, including an early discussion considering both medical and surgical treatment as options, could positively influence operative outcomes.
Role of Lower Esophageal Squamous Cell Carcinoma Margin Location on Abdominal Lymph Node Metastasis Risk
Authors: Zhong Xia , Tu Xue-Hua , A-Lai Gu-Ha , Zhuo Ze-Guo , Yao Peng , Zhang Ying , Xu Zhi-Jie , Lin Yi-Dan
Published: April 2023

Background: Different sites of esophageal cancer are accompanied by different regional lymph node metastasis (LNM) risks. We aimed to investigate the impact of a lower tumor margin on abdominal LNM risk. Methods: We enrolled patients who underwent esophagectomy for esophageal squamous carcinoma (ESCC) from 2014 to 2017 in West China Hospital. Overall survival (OS) analysis was performed. We measured the distance between the lower tumor margin and esophagogastric junction (LED) with upper gastrointestinal contrast-enhanced X-ray (UGCXR). Multivariate logistic regression analysis and propensity score matching (PSM) were performed to explore the relationship between LED and the risk of abdominal LNM. Abdominal LNM risk in ESCC was stratified based on the location of the lower tumor margin. A model predicting abdominal LNM risk was constructed and presented with a nomogram. Results: The included patients had an abdominal LNM rate of 48.29%. In multivariate logistic regression analysis, LED was identified as a risk factor for abdominal LNM. Subgroup analysis of middle ESCC showed that patients with an LED less than 10 cm had a significantly higher rate of abdominal LNM than those with an LED greater than 10 cm. The abdominal LNM rate in middle ESCC patients with an LED less than 10 cm was 32.2%, while it was 35.1% in lower ESCC patients whose lower tumor margin did not invade the esophagogastric junction (EGJ), which was comparable after PSM. Conclusions: LED could help surgeons evaluate the risk of abdominal LNM preoperatively and better guide dissection of abdominal lymph nodes according to risk level.
Surveying of Nearshore Bathymetry Using UAVs Video Stitching
Authors: Fan Jinchang , Pei Hailong , Lian Zengjie
Published: April 2023

In this paper, we extended video stitching to nearshore bathymetry for videos that were captured for the same coastal field simultaneously by two unmanned aerial vehicles (UAVs). In practice, a video captured by a single UAV often shows a limited coastal zone with a lack of a wide field of view. To solve this problem, we proposed a framework in which video stitching and bathymetric mapping were performed in sequence. Specifically, our method listed the video acquisition strategy and took two overlapping videos captured by two UAVs as inputs. Then, we adopted a unified video stitching and stabilization optimization to compute the stitching and stabilization of one of the videos separately. In this way, we can obtain the best stitching result. At the same time, background feature points identification on the shore plays the role of short-time visual odometry. Through the obtained panoramic video in Shuang Yue Bay, China, we used the temporal cross-correlation analysis based on the linear dispersion relationship to estimate the water depth. We selected the region of interest (ROI) area from the panoramic video, performed an orthorectification transformation and extracted time-stack images from it. The wave celerity was then estimated from the correlation of the signal through filtering processes. Finally, the bathymetry results were compared with the cBathy. By applying this method to two UAVs, a wider FOV was created and the surveying area was expanded, which provided effective input data for the bathymetry algorithms.
User-Driven Design and Development of an Underwater Soft Gripper for Biological Sampling and Litter Collection
Authors: Picardi Giacomo , De Luca Mauro , Chimienti Giovanni , Cianchetti Matteo , Calisti Marcello
Published: April 2023

Implementing manipulation and intervention capabilities in underwater vehicles is of crucial importance for commercial and scientific reasons. Mainstream underwater grippers are designed for the heavy load tasks typical of the industrial sector; however, due to the lack of alternatives, they are frequently used in biological sampling applications to handle irregular, delicate, and deformable specimens with a consequent high risk of damage. To overcome this limitation, the design of grippers for marine science applications should explicitly account for the requirements of end-users. In this paper, we aim at making a step forward and propose to systematically account for the needs of end-users by resorting to design tools used in industry for the conceptualization of new products which can yield great benefits to both applied robotic research and marine science. After the generation of the concept design for the gripper using a reduced version of the House of Quality and the Pugh decision matrix, we reported on its mechanical design, construction, and preliminary testing. The paper reports on the full design pipeline from requirements collection to preliminary testing with the aim of fostering and providing structure to fruitful interdisciplinary collaborations at the interface of robotics and marine science.
Correlation of Vitamin 25(OH)D, Liver Enzymes, Potassium, and Oxidative Stress Markers with Lipid Profile and Atheromatic Index: A Pilot Study
Authors: Ioannidou Stavroula , Kazeli Konstantina , Ventouris Hristos , Amanatidou Dionysia , Gkinoudis Argyrios , Lymperaki Evgenia
Published: April 2023

According to recent literature, there is a limited amount of data about the correlation of vitamin 25(OH)D, potassium (K), oxidative stress parameters, and other biomarkers with dyslipidemia, which is an established risk factor for cardiovascular diseases (CVDs). This study aims to investigate the correlation of lipid profile and atheromatic index TC/HDL with several biomarkers and oxidative stress parameters. A total of 102 volunteers, 67 with atheromatic index TC/HDL > 3.5 (Group A) and 35 with TC/HDL < 3.5 (Group B), aged from 26 to 78 years, participated in this study. Serum levels of triglycerides (TG), total cholesterol (TC), low- and high-density lipoproteins (LDL and HDL), vitamin 25(OH)D [25(OH)D], potassium (K), sodium (Na), lactose dehydrogenase (LDH), liver enzymes including serum glutamic oxaloacetic and glutamic pyruvic transaminases (SGOT and SGPT), gamma-glutamyl transferase (γ-GT), and alkaline phosphatase (ALP) were analyzed using standard photometric methods. Oxidative stress parameters such as reactive oxygen species (ROS) were detected with fluorometric methods, whereas total oxidative (TOS) and antioxidative status (TAS) were measured with spectrophotometric methods. According to the results, negative correlations of HDL (r = −0.593) and 25(OH)D (r = −0.340) and K (r = −0.220) were found, and positive expected correlations of LDL (r = 0.731), TC (r = 0.663), and TG (r = 0.584) with atheromatic index in the total studied sample were found. In conclusion, patients with a dyslipidemic profile should frequently check not only their lipid profile but also other biomarkers such as 25(OH)D, potassium, and oxidative stress markers to predict dyslipidemia and avoid subsequent disorders.
Exploring the Effects of Municipal Land and Building Policies on Apartment Size in New Residential Construction in Sweden
Authors: Engerstam Sviatlana , Warsame Abukar , Wilhelmsson Mats
Published: April 2023

New residential construction in many countries with rapid urban growth is often interrelated with smaller housing units being built. Sweden is not an exception. It is of interest to investigate the driving forces behind this tendency. Our presumption is that municipal land price policies and building permit regulations might play a certain role in this process. Contrary to previous studies that focus on the number of new dwelling units in housing construction, our purpose is to analyze the average size of new housing units and the factors that affect it on an aggregate level. We apply seemingly unrelated regressions for analysis of the average apartment size in new residential construction in the three largest metropolitan regions in Sweden as a function of the changes in population, apartment rent and prices, mortgage interest rates, land prices, and building permits per capita as a proxy for regulation. The unbalanced panel dataset includes the period between 1998 and 2017 and covers both the rental and the housing cooperative sectors. The analysis demonstrates that land prices and building policies along with market fundamentals are the underlying factors that affect the average size of an apartment in new residential construction in Sweden.
Online Marketing of European Geoparks as a Landscape Promotion Tool
Authors: Molokáč Mário , Kornecká Enikő , Pavolová Henrieta , Bakalár Tomáš , Jesenský Miloš
Published: April 2023

In the current post-COVID-19 era, online marketing is a supporting tool in marketing. The potential visitors to tourism destinations, such as geoparks, are increasingly guided and oriented by the rules of online marketing as the online space represents the main source of information. Geoparks should take this as a key fact. The research concentrates on the issue of geopark marketing from the view of the available information that geoparks present in the online space. The fundamental is to point out the information that is available on geopark websites. The method used in the research is based on an evaluation matrix that served as a basis for searching for information on websites. Based on the results of the analysis, geoparks in the EGN network present information of a general nature to a sufficient extent on their websites; they lack a better offer of product packages, online sales and orientation to new social networks that are used by the young generation. The clear answer is the professionalization of marketing, which would undoubtedly represent an effective step in the management of geoparks as well as the promotion of the country that the geopark represents.
Knowledge of the Sky among Indigenous Peoples of the South American Lowlands—First Archaeoastronomical Analyses of Orientations at Mounds in Uruguay
Authors: Gianotti Camila , González-García A. César , Gazzán Nicolás , Cancela-Cereijo Cristina , Sotelo Moira
Published: April 2023

We analyzed, from a cultural astronomy perspective, the relationship between the orientations of five mound sites and different astronomical events in the lowland region of Uruguay. We found significant relationships between the orientations of the mounds and the Southern Cross/Milky Way and the full Moon during the winter solstice ca. 3000 years BP. These relationships, meanings and senses to different native peoples of South America were explored from the literature of travelers’ and naturalists’ chronicles, alongside the ethnohistorical, ethnographic and archaeological literature. In particular, we highlighted the link among those peoples of the area of the Southern Cross/ Milky Way with a mythical Ñandú (Rhea americana). Such an interpretation has allowed us to raise the possibility that we are being faced with the integration of knowledge of the sky in the form of the social construction of inhabited space and the configuration of the landscape.
Developing Sustainable Behaviors for Underground Heritage Tourism Management: The Case of Persian Qanats, a UNESCO World Heritage Property
Authors: Mousazadeh Hossein , Ghorbani Amir , Azadi Hossein , Almani Farahnaz Akbarzadeh , Zangiabadi Ali , Zhu Kai , Dávid Lóránt Dénes
Published: April 2023

The Persian Qanats, ancient underground aqueduct systems that have provided irrigation water to arid regions in Iran for over 3000 years, are recognized as a vital element of the country’s cultural heritage. Eleven of these impressive structures have been included on the UNESCO World Heritage List, underlining their cultural, social, political, and physical significance. As these underground heritage sites offer a unique opportunity for fostering territorial collaboration and social capital between tourists and the local community, it is important to develop a comprehensive model to influence the behavior of tourists, officials, and natives in their treatment of these invaluable sites. This study aimed to design a sustainable behavior model for underground heritage tourism management in Persian Qanats that have been listed as UNESCO World Heritage properties. The research employed a theme analysis approach, utilizing a statistical sample of 22 base managers and Qanat tourism experts. The study used content and thematic analysis to develop a research model that guarantees the sustainable protection of these valuable underground heritage sites by shaping the behavior of tourists, officials, and natives. The proposed model serves as a guideline for effective behavior management, leading to the preservation of these significant underground heritage sites for future generations. In conclusion, this study provides valuable insights into the sustainable management of Persian Qanats and the promotion of a strong sense of territorial collaboration and social capital between tourists and the local community.