bluuebunny/crossref_metadata_2025 huggingface dataset:
Online Pornography Seeking Behavior and Its Relation to Literacy Rate and Financial Status of Indian States
Authors: Mondal Shaikat , Mondal Himel
Published: April 2020

Background: Pornography has become widely accessible due to the popularization of smartphones and internet connectivity. Consuming pornography has multiple effects on an individual and on society. Our research question was if pornography-seeking behavior has any correlation with education and financial status in Indian states.

Objective: To find the online pornography-seeking behavior of Indian internet users according to states and find its correlation with the literacy level and per capita net state domestic product (NSDP).

Methods: Trends of internet search about pornography-related keywords (namely, porn, XXX, Xvideos, and sex) were obtained from a public domain https://trends.google.com/trends . State-wise relative search volumes were compared with the literacy rate and NSDP of the state. Pearson correlation coefficients were calculated with the help of GraphPad Prism 6.01 (GraphPad Software, Inc., California, USA). A P < .05 was considered statistically significant.

Results: Northeastern states showed a higher volume of overall online pornography searches. There was no significant correlation between the literacy rate and relative search volumes of pornography-related keywords. Overall pornography-related search volume showed a significant negative ( r = –0.49, P = .003) correlation with NSDP.

Conclusion: Online pornography seeking behaviors in Indian states are not related to the literacy rate of the state. A higher volume of searches is from states with lower NSDP. The underlying factors for this finding should be explored in a future study.
Small for gestational age is a risk factor for thyroid dysfunction in preterm newborns
Authors: Liu Chunhua , Wang Kaiyan , Guo Jizhong , Chen Jiru , Chen Mei , Xie Zhexi , Chen Pu , Wu Beiyan , Lin Niyang
Published: April 2020

Background

Thyroid hormones play an important role in the normal growth and maturation of the central nervous system. However, few publications addressed the altered thyroid hormone levels in preterm small for gestational age (SGA) newborns. We hypothesized preterm SGA infants have higher thyroid-stimulating hormone (TSH) concentrations than appropriate for gestational age (AGA) ones within the normal range and an increased incidence of thyroid dysfunction.

Methods

The study was designed to compare thyroid hormone levels within the normal range and the incidence of thyroid dysfunction in the SGA and AGA groups to test the hypothesis. The medical records of all preterm infants admitted to the neonatal intensive care unit (NICU) at the First Affiliated Hospital of Shantou University Medical College, Shantou, China, between January 1, 2015 and December 31, 2018, were reviewed. Blood samples were collected between 72 and 96 h of life and analyzed with TSH, free thyroxine (FT4) and free triiodothyronine (FT3) assays. Thyroid function test (TFT) results, and neonatal demographic and clinical factors were analyzed to identify the associations between SGA birth and altered thyroid concentrations and thyroid dysfunction.

Results

TSH and FT4 concentrations were significantly higher in the SGA group than the AGA group ((3.74(interquartile range (IQR):2.28 ~ 6.18) vs. 3.01(IQR: 1.81 ~ 5.41) mU/L, p = 0.018), and (17.76 ± 3.94 vs. 17.42 ± 3.71 pmol/L, p = 0.371), respectively). The higher TSH levels were associated with being SGA or Z-score of birth weight (BW) for GA after adjusting for potential confounders ((βSGA = 0.68 (95% confidence interval (CI) 0.15 ~ 1.21), p = 0.013) or (βZ-score = − 0.25 (95%CI -0.48 ~ − 0.03), p = 0.028), respectively). However, we did not find a significant association between SGA birth and altered FT4 concentrations. Furthermore, compared with the AGA group, the SGA group presented an increased incidence of transient hypothyroxinemia with delayed TSH elevation (dTSHe), a higher percentage receiving levothyroxine (L-T4) therapy, and a higher rate of follow-up within the first 6 months of life.

Conclusions

Preterm SGA newborns had significantly higher TSH concentrations within the normal range and an increased incidence of thyroid dysfunction. The SGA newborns with these features should be closely followed up with periodical TFTs and endocrinologic evaluation.
Can mathematical modelling solve the current Covid-19 crisis?
Authors: Panovska-Griffiths Jasmina
Published: April 2020

Since COVID-19 transmission started in late January, mathematical modelling has been at the forefront of shaping the decisions around different non-pharmaceutical interventions to confine its’ spread in the UK and worldwide. This Editorial discusses the importance of modelling in understanding Covid-19 spread, highlights different modelling approaches and suggests that while modelling is important, no one model can give all the answers.
Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies
Authors: Wu Tong , Liu Jinfeng , Li Meijie , Zhang Ge , Liu Lijuan , Li Xing , Men Xiao , Xian Mo , Zhang Haibo
Published: April 2020

Background

Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source. However, the efficiency and titers of this method are limited by the low host tolerance to sabinene (in both eukaryotes and prokaryotes).

Results

In this study,Escherichia coliBL21(DE3) was selected as the strain for adaptive laboratory evolution. The strain was evolved by serial passaging in the medium supplemented with gradually increasing concentration of sabinene, and the evolved strain XYF(DE3), which exhibited significant tolerance to sabinene, was obtained. Then, XYF(DE3) was used as the host for sabinene production and an 8.43-fold higher sabinene production was achieved compared with the parental BL21(DE3), reaching 191.76 mg/L. Whole genomes resequencing suggested the XYF(DE3) strain is a hypermutator. A comparative analysis of transcriptomes of XYF(DE3) and BL21(DE3) was carried out to reveal the mechanism underlying the improvement of sabinene tolerance, and 734 up-regulated genes and 857 down-regulated genes were identified. We further tested the roles of the identified genes in sabinene tolerance via reverse engineering. The results demonstrated that overexpressions ofybcKgene of the DLP12 family, the inner membrane protein geneygiZ, and the methylmalonyl-CoA mutase genescpAcould increase sabinene tolerance of BL21(DE3) by 127.7%, 71.1%, and 75.4%, respectively. Furthermore, scanning electron microscopy was applied to monitor cell morphology. Under sabinene stress, the parental BL21(DE3) showed increased cell length, whereas XYF(DE3) showed normal cell morphology. In addition, overexpression ofybcK,ygiZorscpAcould partially rescue cell morphology under sabinene stress and overexpression ofygiZorscpAcould increase sabinene production in BL21(DE3).

Conclusions

This study not only obtained a sabinene-tolerant strain for microbial production of sabinene but also revealed potential regulatory mechanisms that are important for sabinene tolerance. In addition, for the first time,ybcK, ygiZ, andscpAwere identified to be important for terpene tolerance inE. coliBL21(DE3).
Enhanced rhamnolipids production using a novel bioreactor system based on integrated foam-control and repeated fed-batch fermentation strategy
Authors: Xu Ning , Liu Shixun , Xu Lijie , Zhou Jie , Xin Fengxue , Zhang Wenming , Qian Xiujuan , Li Min , Dong Weiliang , Jiang Min
Published: April 2020

Background

Rhamnolipids are the best known microbial-derived biosurfactants, which has attracted great interest as potential ‘‘green” alternative for synthetic surfactants. However, rhamnolipids are the major contributors to severe foam problems, which greatly inhibit the economics of industrial-scale production. In this study, a novel foam-control system was established for ex situ dealing with the massive overflowing foam. Based on the designed facility, foam reduction efficiency, rhamnolipids production by batch and repeated fed-batch fermentation were comprehensively investigated.

Results

An ex situ foam-control system was developed to control the massive overflowing foam and improve rhamnolipids production. It was found that the size of individual bubble in the early stage was much larger than that of late fermentation stage. The foam liquefaction efficiency decreased from 54.37% at the beginning to only 9.23% at the end of the fermentation. This difference of bubble stability directly resulted in higher foam reduction efficiency of 67.46% in the early stage, whereas the small uniform bubbles can only be reduced by 57.53% at the later fermentation stage. Moreover, reduction of secondary foam is very important for foam controlling. Two improved designs of the device in this study obtained about 20% improvement of foam reduction efficiency, respectively. The batch fermentation result showed that the average volume of the overflowing foam was reduced from 58–640 to 19–216 mL/min during the fermentation process, presenting a notable reduction efficiency ranging from 51.92 to 73.47%. Meanwhile, rhamnolipids production of batch fermentation reached 45.63 g/L, and the yield 0.76 g/g was significantly better than ever reported. Further, a repeated fed-batch fermentation based on the overall optimization was carried out. Total rhamnolipids concentration reached 48.67 g/L with the yield around of 0.67–0.83 g/g, which presented an improvement of 62% and 49% compared with conventional batch fermentation by using various kinds of defoamers, respectively.

Conclusions

The ex situ foam-control system presented a notable reduction efficiency, which helped greatly to easily solve the severe foaming problem without any defoamer addition. Moreover, rhamnolipids production and yield by repeated fed-batch fermentation obtained prominent improvement compared to conventional batch cultivation, which can further facilitate economical rhamnolipids production at large scales.
Higher efficacy of rupatadine 20 mg and 10 mg versus placebo in patients with perennial allergic rhinitis: a pooled responder analysis
Authors: Valero Antonio , Izquierdo Iñaki , Kowalski Marek L. , Scadding Glenis K. , Bousquet Jean , Mullol Joaquim
Published: April 2020

Background

The clinical efficacy of rupatadine in terms of responders has not been previously explored in perennial allergic rhinitis (PAR).

Methods

This pooled analysis included data from 6 randomised, double-blind, placebo-controlled trials conducted in PAR patients treated with rupatadine 10 mg or 20 mg, or placebo. Participants were aged ≥ 18 years, with diagnosis of PAR and a Total 4 Nasal Symptom Score (T4NSS) ≥ 5. We evaluated the T4NSS and Total 5 Symptom Score (T5SS) for 28 days of treatment, the responder proportion (50% and 75% response), and the time to response.

Results

Efficacy data from 1486 patients were analysed: 585 received placebo, 682 rupatadine 10 mg, and 219 rupatadine 20 mg. Compared with placebo, rupatadine promoted greater symptom improvements and higher responder proportions (50% and 75% response) for T4NSS and T5SS over 28 days. Symptom improvements and responder proportions were higher in the rupatadine 20 mg group vs the 10 mg group. The time to response was shorter in the rupatadine 20 mg group vs the 10 mg group for T4NSS (16 and 9 days for the 50% and 75% responses, respectively) and for T5SS (13 and 8 days for the 50% and 75% responses, respectively).

Conclusions

Rupatadine was efficacious in reducing allergic rhinitis symptoms, showing high responder proportions. The faster and stronger effect of rupatadine 20 mg may suggest its use in patients with severe PAR or not responding to the standard dose.
The impact of social, national and community-based health insurance on health care utilization for mental, neurological and substance-use disorders in low- and middle-income countries: a systematic review
Authors: Docrat Sumaiyah , Besada Donela , Cleary Susan , Lund Crick
Published: April 2020

Background

Whilst several systematic reviews conducted in Low- and Middle-Income Countries (LMICs) have revealed that coverage under social (SHI), national (NHI) and community-based (CBHI) health insurance has led to increased utilization of health care services, it remains unknown whether, and what aspects of, these shifts in financing result in improvements to mental health care utilization. The main aim of this review was to examine the impact of SHI, NHI and CBHI enrollment on mental health care utilization in LMICs.

Methods

Systematic searches were performed in nine databases of peer-reviewed journal articles: Pubmed, Scopus, SciELO via Web of Science, Africa Wide, CINAHL, PsychInfo, Academic Search Premier, Health Source Nursing Academic and EconLit for studies published before October 2018. The quality of the studies was assessed using the Effective Public Health Practice Project quality assessment tool for quantitative studies. The systematic review was reported according to the PRISMA guidelines (PROSPERO;2018; CRD42018111576).

Results

Eighteen studies were included in the review. Despite some heterogeneity across countries, the results demonstrated that enrollment in SHI, CBHI and NHI schemes increased utilization of mental health care. This was consistent for the length of inpatient admissions, number of hospitalizations, outpatient use of rehabilitation services, having ever received treatment for diagnosed schizophrenia and depression, compliance with drug therapies and the prescriptions of more favorable medications and therapies, when compared to the uninsured. The majority of included studies did not describe the insurance schemes and their organizational details at length, with limited discussion of the links between these features and the outcomes. Given the complexity of mental health service utilization in these diverse contexts, it was difficult to draw overall judgements on whether the impact of insurance enrollment was positive or negative for mental health care outcomes.

Conclusions

Studies that explore the impact of SHI, NHI and CBHI enrollment on mental health care utilization are limited both in number and scope. Despite the fact that many LMICs have been hailed for financing reforms towards universal health coverage, evidence on the positive impact of the reforms on mental health care utilization is only available for a small sub-set of these countries.
Exploration of isoxanthohumol bioconversion from spent hops into 8-prenylnaringenin using resting cells of Eubacterium limosum
Authors: Moens Esther , Bolca Selin , Van de Wiele Tom , Van Landschoot Anita , Goeman Jan L. , Possemiers Sam , Verstraete Willy
Published: April 2020

Hops is an almost unique source of the potent phytoestrogen 8-prenylnaringenin (8-PN). As hops contain only low levels of 8-PN, synthesis may be more attractive than extraction. A strain of the Gram-positive Eubacterium limosum was isolated previously for 8-PN production from more abundant precursor isoxanthohumol (IX) from hops. In this study, spent hops, an industrial side stream from the beer industry, was identified as interesting source of IX. Yet, hop-derived compounds are well-known antibacterial agents and the traces of a large variety of different compounds in spent hops interfered with growth and IX conversion. Critical factors to finally enable bacterial 8-PN production from spent hops, using a food and feed grade medium, were evaluated in this research. The use of bacterial resting cells and complex medium at a pH of 7.8–8 best fulfilled the requirements for 8-PN production and generated a solid basis for development of an economic process.
Synthesis, characterization and assessment of anti-quorum sensing activity of copper(II)-ciprofloxacin complex against Pseudomonas aeruginosa PAO1
Authors: Rafiee Farzaneh , Haghi Fakhri , Bikas Rahman , Heidari Azam , Gholami Mina , Kozakiewicz Anna , Zeighami Habib
Published: April 2020

Quorum sensing (QS) inhibition by metal-antibiotic complexes is a promising strategy for the management and control of multidrug resistant Pseudomonas aeruginosa infections. We investigated the anti-quorum sensing activity of sub-minimum inhibitory concentration (sub-MIC) of copper(II) sulfate pentahydrate-ciprofloxacin (Cu-CIP) complex and free ciprofloxacin (free-CIP) against P. aeruginosa PAO1. Copper-CIP complex was synthesized and its characterization was assessed using spectroscopic methods and single crystal X-ray analysis. The effect of sub-MIC (1/4 and 1/16 MIC) concentrations of Cu-CIP and free-CIP on cell growth, biofilm formation, motility, alginate and pyocyanin production, H2O2 susceptibility and expression of QS circuit genes lasI and lasR in PAO1 was determined. Minimum inhibitory concentration of Cu-CIP complex and free-CIP was determined as 0.125 µg/ml. Copper-CIP complex did not show significant effect on the cell growth at concentrations of 1/4 and 1/16 MIC. However, sub-MIC concentrations (1/4 and 1/16 MIC) of Cu-CIP showed the significant reduction in violacein production, motility, biofilm formation, alginate and pyocyanin production and sensitivity to H2O2 in a concentration dependent manner (P < 0.001). Copper-CIP at the concentration of 1/4 MIC showed the greatest reduction in lasI and lasR transcriptional expression (89.5% and 96.2% respectively). Considering the biological effects of Cu-CIP complex and its inhibitory activity on QS related virulence traits at low concentrations (0.03 and 0.007 µg/ml), it may be used as an effective approach in the management of infections caused by P. aeruginosa.
School-going transgender youths’ experiences at health care facilities: a systematic scoping review protocol
Authors: Mulqueeny Delarise M. , Nkabini Senzelokuhle M. , Mashamba-Thompson Tivani P.
Published: April 2020

Background

Globally, miniscule improvements have been implemented regarding equality, inclusion, access, and protection of people with diverse gender identities whilst accessing health care facilities of which transgender youth form part. Literature has highlighted that the care transgender youth receive at health care facilities can result in positive or negative outcomes. School-going transgender youth constitute a unique group whose experiences at health care facilities warrant ongoing research. Hence, the objective of this scoping review is to systematically map evidence of school-going transgender youths’ experiences at health care facilities regarding service delivery, support, and policies and to identify literature gaps that could inform future research.

Methods

We will conduct a scoping review, using peer-reviewed journal articles that present literature on school-going transgender youths’ experiences at health care facilities. Searches for relevant articles will be conducted on the following databases: PubMed, Cochrane Library, Campbell collaboration, Scopus, Embase, and MEDLINE. Additional searches will be conducted on institutional websites or web-based search portals. Two reviewers will independently extract data from all relevant search engines incorporating the study objective, research questions, and eligibility criteria. The inclusion criteria include published full-text qualitative, quantitative, and mixed-method studies that address the topic with no language and publication year limitations to reflect a comprehensive range of literature that includes the implementation of the SDGs. All literature that does not meet the inclusion criteria will be excluded. The quality of included studies will be appraised using the mixed methods appraisal tool (MMAT) – version 2018.

Discussion

We anticipate mapping the experiences of school-going transgender youth at health care facilities. Once summarized, the data could be useful to clinical educators, health workers, policy makers, and guide future research to ensure that the human and patients’ rights of transgender youth, are globally acknowledged, protected, and respected within health care facilities.
Human iPSCs derived astrocytes rescue rotenone-induced mitochondrial dysfunction and dopaminergic neurodegeneration in vitro by donating functional mitochondria
Authors: Cheng Xiao-Yu , Biswas Sangita , Li Juan , Mao Cheng-Jie , Chechneva Olga , Chen Jing , Li Kai , Li Jiao , Zhang Jin-Ru , Liu Chun-Feng , Deng Wen-Bin
Published: April 2020

Background

Parkinson’s disease (PD) is one of the neurodegeneration diseases characterized by the gradual loss of dopaminergic (DA) neurons in the substantia nigra region of the brain. Substantial evidence indicates that at the cellular level mitochondrial dysfunction is a key factor leading to pathological features such as neuronal death and accumulation of misfolded α-synuclein aggregations. Autologous transplantation of healthy purified mitochondria has shown to attenuate phenotypes in vitro and in vivo models of PD. However, there are significant technical difficulties in obtaining large amounts of purified mitochondria with normal function. In addition, the half-life of mitochondria varies between days to a few weeks. Thus, identifying a continuous source of healthy mitochondria via intercellular mitochondrial transfer is an attractive option for therapeutic purposes. In this study, we asked whether iPSCs derived astrocytes can serve as a donor to provide functional mitochondria and rescue injured DA neurons after rotenone exposure in an in vitro model of PD.

Methods

We generated DA neurons and astrocytes from human iPSCs and hESCs. We established an astroglial-neuronal co-culture system to investigate the intercellular mitochondrial transfer, as well as the neuroprotective effect of mitochondrial transfer. We employed immunocytochemistry and FACS analysis to track mitochondria.

Results

We showed evidence that iPSCs-derived astrocytes or astrocytic conditioned media (ACM) can rescue DA neurons degeneration via intercellular mitochondrial transfer in a rotenone induced in vitro PD model. Specifically, we showed that iPSCs-derived astrocytes from health spontaneously release functional mitochondria into the media. Mito-Tracker Green tagged astrocytic mitochondria were detected in the ACM and were shown to be internalized by the injured neurons via a phospho-p38 depended pathway. Transferred mitochondria were able to significantly reverse DA neurodegeneration and axonal pruning following exposure to rotenone. When rotenone injured neurons were cultured in presence of ACM depleted of mitochondria (by ultrafiltration), the neuroprotective effects were abolished.

Conclusions

Our studies provide the proof of principle that iPSCs-derived astrocytes can act as mitochondria donor to the injured DA neurons and attenuate pathology. Using iPSCs derived astrocytes as a donor can provide a novel strategy that can be further developed for cellular therapy for PD.
False data injection attack (FDIA): an overview and new metrics for fair evaluation of its countermeasure
Authors: Ahmed Mohiuddin , Pathan Al-Sakib Khan
Published: April 2020

The concept of false data injection attack (FDIA) was introduced originally in the smart grid domain. While the term sounds common, it specifically means the case when an attacker compromises sensor readings in such tricky way that undetected errors are introduced into calculations of state variables and values. Due to the rapid growth of the Internet and associated complex adaptive systems, cyber attackers are interested in exploiting similar attacks in other application domains such as healthcare, finance, defense, governance, etc. In today’s increasingly perilous cyber world of complex adaptive systems, FDIA has become one of the top-priority issues to deal with. It is a necessity today for greater awareness and better mechanism to counter such attack in the cyberspace. Hence, this work presents an overview of the attack, identifies the impact of FDIA in critical domains, and talks about the countermeasures. A taxonomy of the existing countermeasures to defend against FDIA is provided. Unlike other works, we propose some evaluation metrics for FDIA detection and also highlight the scarcity of benchmark datasets to validate the performance of FDIA detection techniques.
Product experiences of clothing attachment in baby boomers in the United States
Authors: Ceballos Lina M. , Min Seoha
Published: April 2020

Despite the importance of the baby boomer generation, there is relatively scarce research focused on that consumer group. Based on the framework of product experience, this study explores the experiences of older baby boomers associated with consumer-clothing attachment. The interpretive approach utilized in-depth interviews with 18 older baby boomers born between 1946 and 1955 to enquire about their experiences with attached clothing. Participants were asked to bring to the interview photographs of the clothing they had become more attached to. Findings indicate that the experiences of older baby boomers with attached clothing are mainly created, developed, and maintained via all three dimensions of product experience; aesthetic properties of the product, positive emotions triggered by the product, and the symbolic and instrumental meanings associated with the product. However, the aesthetic properties and positive emotions related to those products were bound to its assigned meanings. Meaning varied and was classified as associations with: identity or the belief that the object is a self-extension; memories or the connection with the past; social standing or sense of status; and strong associations with utility. For all participants, the stronger the experience of meaning with a specific product, the stronger the level of attachment towards that product. This qualitative investigation extends the understanding of the framework of product experience and the concept of consumer-clothing attachment. Contributions offer opportunities to marketers and designers who seek to better understand the experiences behind baby boomers’ clothing attachment.
Multiresolution Application of Artificial Intelligence in Digital Pathology for Prediction of Positive Lymph Nodes From Primary Tumors in Bladder Cancer
Authors: Harmon Stephanie A. , Sanford Thomas H. , Brown G. Thomas , Yang Chris , Mehralivand Sherif , Jacob Joseph M. , Valera Vladimir A. , Shih Joanna H. , Agarwal Piyush K. , Choyke Peter L. , Turkbey Baris
Published: April 2020

PURPOSE

To develop an artificial intelligence (AI)–based model for identifying patients with lymph node (LN) metastasis based on digital evaluation of primary tumors and train the model using cystectomy specimens available from The Cancer Genome Atlas (TCGA) Project; patients from our institution were included for validation of the leave-out test cohort.

METHODS

In all, 307 patients were identified for inclusion in the study (TCGA, n = 294; in-house, n = 13). Deep learning models were trained from image patches at 2.5×, 5×, 10×, and 20× magnifications, and spatially resolved prediction maps were combined with microenvironment (lymphocyte infiltration) features to derive a final patient-level AI score (probability of LN metastasis). Training and validation included 219 patients (training, n = 146; validation, n = 73); 89 patients (TCGA, n = 75; in-house, n = 13) were reserved as an independent testing set. Multivariable logistic regression models for predicting LN status based on clinicopathologic features alone and a combined model with AI score were fit to training and validation sets.

RESULTS

Several patients were determined to have positive LN metastasis in TCGA (n = 105; 35.7%) and in-house (n = 3; 23.1%) cohorts. A clinicopathologic model that considered using factors such as age, T stage, and lymphovascular invasion demonstrated an area under the curve (AUC) of 0.755 (95% CI, 0.680 to 0.831) in the training and validation cohorts compared with the cross validation of the AI score (likelihood of positive LNs), which achieved an AUC of 0.866 (95% CI, 0.812 to 0.920; P = .021). Performance in the test cohort was similar, with a clinicopathologic model AUC of 0.678 (95% CI, 0.554 to 0.802) and an AI score of 0.784 (95% CI, 0.702 to 0.896; P = .21). In addition, the AI score remained significant after adjusting for clinicopathologic variables ( P = 1.08 × 10−9), and the combined model significantly outperformed clinicopathologic features alone in the test cohort with an AUC of 0.807 (95% CI, 0.702 to 0.912; P = .047).

CONCLUSION

Patients who are at higher risk of having positive LNs during cystectomy can be identified on primary tumor samples using novel AI-based methodologies applied to digital hematoxylin and eosin–stained slides.
Dynein-mediated microtubule translocation powering neurite outgrowth in chick and Aplysia neurons requires microtubule assembly
Authors: McElmurry Kristi , Stone Jessica E. , Ma Donghan , Lamoureux Phillip , Zhang Yueyun , Steidemann Michelle , Fix Lucas , Huang Fang , Miller Kyle E. , Suter Daniel M.
Published: April 2020

Previously, we have shown that bulk microtubule (MT) movement correlates with neurite elongation, and blocking either dynein activity or MT assembly inhibits both processes. However, whether the contributions of MT dynamics and dynein activity to neurite elongation are separate or interdependent is unclear. Here, we investigated the underlying mechanism by testing the roles of dynein and MT assembly in neurite elongation of Aplysia and chick neurites using time-lapse imaging, fluorescent speckle microscopy, super-resolution imaging and biophysical analysis. Pharmacologically inhibiting either dynein activity or MT assembly reduced neurite elongation rates as well as bulk and individual MT anterograde translocation. Simultaneously suppressing both processes did not have additive effects, suggesting a shared mechanism of action. Single-molecule switching nanoscopy revealed that inhibition of MT assembly decreased the association of dynein with MTs. Finally, inhibiting MT assembly prevented the rise in tension induced by dynein inhibition. Taken together, our results suggest that MT assembly is required for dynein-driven MT translocation and neurite outgrowth.
FRACTAL GEOMETRY AND QUARK-GLUON PLASMA PHYSICS
Authors: Antoniou N. G.
Published: April 2020

Incorporating fractal geometry in the Regge-Mueller approach to strong interaction dynamics one may formulate a model for the one-dimensional critical sector of the hadronic 5-matrix in a high energy collision. A non conventional component of the correlation functions in rapidity space is obtained, the phenomenological implications of which are related with the intermittency effects in quark-gluon plasma physics.
GoodVibes: automated thermochemistry for heterogeneous computational chemistry data
Authors: Luchini Guilian , Alegre-Requena Juan V. , Funes-Ardoiz Ignacio , Paton Robert S.
Published: April 2020

GoodVibes is an open-source Python toolkit for processing the results of quantum chemical calculations. Thermochemical data are not simply parsed, but evaluated by evaluation of translational, rotational, vibrational and electronic partition functions. Changes in concentration, pressure, and temperature can be applied, and deficiencies in the rigid rotor harmonic oscillator treatment can be corrected. Vibrational scaling factors can also be applied by automatic detection of the level of theory and basis set. Absolute and relative thermochemical values are output to text and graphical plots in seconds. GoodVibes provides a transparent and reproducible way to process raw computational data into publication-quality tables and figures without the use of spreadsheets.
Case Report: Sudden death related to unrecognized cardiac hydatid cyst
Authors: Mesrati Med Amin , Mahjoub Yosra , Ben Abdejlil Nouha , Boussaid Marwa , Belhaj Meriem , Limem Hiba , Chadly Ali , Zakhama Abdelfeteh , Aissaoui Abir
Published: April 2020

Echinococcosis, also known as hydatid disease, is a common parasitic human infestation found in sheep-breeding areas. It is caused by the larvae stage of Echinococcus granulosus, and cysts develop mostly in the lungs and the liver. Cardiac involvement is unusual and silent until acute complications or a fatal outcome occurs. Herein, we report an autopsy case of a young healthy adult who died suddenly. The autopsy revealed an external bulging on the right heart ventricle outlet with a fluid-filled cystic cavity discovered on sectioning. Dissection of other organs did not reveal other cyst locations. Histological examination ascertained the diagnosis of hydatid cyst, and death was attributed to cardiac arrhythmias. Pathologists should keep in mind that hydatid cysts can develop anywhere in the body. Solitary cardiac cyst is rare and can simulate a “silent bomb”. Unfortunately, sudden death remains the frequent manner of revelation of this disease in endemic areas.
MetaboMAPS: Pathway sharing and multi-omics data visualization in metabolic context
Authors: Koblitz Julia , Schomburg Dietmar , Neumann-Schaal Meina
Published: April 2020

Metabolic pathways are an important part of systems biology research since they illustrate complex interactions between metabolites, enzymes, and regulators. Pathway maps are drawn to elucidate metabolism or to set data in a metabolic context. We present MetaboMAPS, a web-based platform to visualize numerical data on individual metabolic pathway maps. Metabolic maps can be stored, distributed and downloaded in SVG-format. MetaboMAPS was designed for users without computational background and supports pathway sharing without strict conventions. In addition to existing applications that established standards for well-studied pathways, MetaboMAPS offers a niche for individual, customized pathways beyond common knowledge, supporting ongoing research by creating publication-ready visualizations of experimental data.
Modernization of Soviet Russia in 1920-1930-ies: transformation programme of the RCP(b) — VKP(b) as instruments of struggle for power
Authors: Anfert'ev Ivan
Published: April 2020

The monograph is devoted to studying the process of implementation of modernization projects of the RCP(b) - VKP(b) 1920-1930-ies in the context of intra-party struggle for power. A lack of managerial experience in the leadership of the country, declared utopian ideas, the bureaucratization of the party-state apparatus and the commitment to radical ways of solving problems gave rise to political and socio-economic crises affect the results. Revealed the limits of the political life of leaders of the ruling party in the implementation of the political-administrative projects considered as a series of unjustified social and economic experiments, criticized the concept of the Soviet state as an apparatus of violence in the interests of the world proletarian revolution.
Intended for specialists in the history of Soviet Russia of the twentieth century, University professors, and for anyone interested in Russian history.
Industrial construction. Buildings and constructions. Corrosion protection and ecology
Authors: Zhukov Aleksey , Astashkin Vladimir , Zholudov Vil'en , Semenov Vyacheslav
Published: April 2020

This monograph summarizes the modern experience of protection of industrial buildings and structures against aggressive impacts are considered characteristic of corrosion processes under the action of liquid, solid and gaseous environments on the main building materials.
Provides a system of regulating the degree of aggressiveness for different parts of buildings and constructions basic provisions for the selection of chemically resistant structures and materials, design methodology section corrosion protection. Systematic design methods of protecting groundwater and soil against aggressive and toxic media, the methods of accounting for the cost of corrosion protection as applied to building elements.
Designed for a wide range of engineering-technical workers (ITR), related to design, construction and exploitation of constructions and structures. Can also be used as a textbook for technical schools, colleges and training system engineers.
Situational training center as an educational environment for the training of competent management personnel
Authors: Khodyakova Nataliya , Mitin Aleksandr
Published: April 2020

The article handles from the standpoint of the situational-environmental approach to the design of educational systems, the substantive and procedural aspects of organizing the activities of educational situational centers, intended for the formation and development of the competencies of management personnel. The educational situational center is interpreted as an educational environment saturated with modern software and hardware, interacting with which the student acquires or improves the experience of making managerial decisions in non-standard and difficult situations, thereby increasing the level of his professional competence. Significant factors of the educational environment of the educational situational center include: conditions that stimulate the development of the reflective and creative experience of managerial specialists; conditions that provide opportunities for students to manifest subjective activity and independence; meaningful conditions that provide a meaning-generating, socio-cultural context of professional activity; conditions for comprehensive (physical, psychological, informational and cultural) safety of students. Each of these groups is assigned the risks of maladjustment of students in the educational environment, which are subject to pedagogical adjustment or dosage, depending on the level of formation of professional and personal experience of management specialists. On the basis of the presented results, conclusions were made regarding the didactic and methodological specifics of teaching in educational situational centers, the peculiarities of the implementation of educational and pedagogical activities in them.
To the question of psychological features of formation of delinquent behavior of the minor
Authors: Teregulova Oxana
Published: April 2020

The article deals with the main social and psychological factors affecting the formation of the personality of a minor offender, as well as delinquent behavior. The interrelation between different types of character accentuations and formation of delinquent behavior of minors is described. The basic prerequisites of delinquent behavior, such as neglect, impunity, shortcomings in the educational process and the social environment are also considered.

The article is based on the data of three focus groups, which discussed the causes and methods of preventing juvenile delinquency. The first focus group was held with the participation of the heads of the DSS Of different regions of Russia. The second was attended by the leaders of PD of different regions of Russia.

Respondents of these groups concluded that impunity, lack of fear and responsibility for their actions is the main factor contributing to the formation of delinquent behavior of minors. Also of great importance is the organization of leisure activities of minors and their involvement in socially useful activities.

Lack of control, a large amount of free time in minors, the lack of realization of their potential are dangerous factors that create the preconditions for the Commission of illegal acts. According to the respondents of both focus groups, it is necessary to pay special attention to the organization of leisure activities of minors, to ensure their employment in various sports, socially useful and creative activities that should be controlled by adults.

The media, especially the Internet, also have a detrimental impact. A detailed description of how and when crimes are committed, how "easy money "is a kind of" textbook of criminal activity» for minors.

In addition, as a prevention of illegal activities of minors, respondents indicated increased penalties.

The second focus group was held with minors aged 15-16 years.

The respondents of this focus group came to the conclusion that the main factor influencing the formation of delinquent behavior of minors is the social environment. They also expressed the opinion on the need for more preventive work and clarification of the law.

Thus, the article considers the main factors and reasons for the formation of delinquent behavior of minors, studied different points of view on this issue and identifies ways to combat this problem.
Professional reliability of engineering: correlation of research approaches
Authors: Balakshina Elena
Published: April 2020

The article contains the results of theoretical analysis on the problem of determining psychological factors of reliability of professional activity of the engineer. This trend is due to the interest and popularity of modern technical professions in the educational and economic spheres, as well as the need to implement tasks to train specialists able to successfully integrate into the production process, to perform job duties qualitatively. The differences and peculiarities of engineering activity are specified on the example of professionals engaged in various labour spheres (industry, education), as well as the spectrum of modern engineering professions, whose activity has significant differences from "standard" technical directions, which require additional attention in the process of disclosure of its psychological content.

It is noted that despite the vast experience in the field of theoretical and practical research of professional activities of different specifications and psychological criteria of evaluation of its quality of development, revealing peculiarities of formation of professional reliability of the specialist very little. Most works pay special attention to the structure of professionally significant qualities or personal-professional qualities, ensuring the safety of activities on the example of professions with public order, public servants and teachers, but the aspects of reliability of engineering are insufficiently and generically represented, which made the developments of this direction relevant.
All of physics on your palm. Interactive reference
Authors: Kuznecov Sergey , Rogozin Konstantin
Published: April 2020

This is a unique visual book created by the best techniques of modern education. It presents the basic laws and formulas for all sections of physics with a huge number of interactive additions, explanations, illustrations, charts, graphs, tables, and drawings, allowing you to learn the material more efficiently. A clear and concise style of writing focuses the reader's attention in the target material, and numerous exercises, control questions and tasks allow you to securely fix in the memory the knowledge.
Additional materials for all sections of General physics course available to You on the Internet in ABS Znanium.com. Using your mobile device, scan the QR code and get it on your smartphone or tablet access to comprehensive information throughout the course of physics in the media formats.
In addition, on the YouTube channels "Salisylate and Isminimal from rocky" (from "the Russian Creative Internet") hosted a large number of additional training materials and videos used in this book.
Interactive Handbook is intended for use in the educational activities of teachers and students of technical specialties of full-time and distance learning forms, as well as students of technical schools and secondary schools.
Examining the Relationship Between Student School Burnout and Problematic Internet Use
Authors: Tomaszek Katarzyna , Muchacka-Cymerman Agnieszka
Published: April 2020

Student burnout and Internet addiction are two of the serious problems that are common among young people. Both are connected with many negative effects on the mental functioning of adolescents. However, so far only few studies have explored these two phenomena all together. The aim of our study was to test the relationship between student burnout (SB) and internet addiction (IA). Specifically, we explored three problems: (i) sex differences in the level of student burnout and problematic Internet use among Polish late adolescents; (ii) correlations between student school burnout and its sub-dimensions and problematic Internet use indicators; (iii) the prediction power of student burnout indicators, quality of social bonds and school functioning characteristics on the level of Internet addiction and its subdimensions. The study was conducted among 230 high school students. The results of the research showed that there were no significant differences between girls and boys in the level of problematic use of the Internet, except that male students assessed their dishonesty about Internet use as higher compared to females. As expected, a higher level of school burnout indicators was significantly connected with a higher level of problematic Internet use. Additionally, school burnout (especially burnout from studying, loss of interest in school and burnout due to parental pressure) and school performance characteristics such as additional classroom lessons and low quality of classmates relationships, significantly predicted the problematic Internet use, which explained 19% of variances in the total score of internet addiction. Our findings confirmed that preventative and interventional strategies developed to reduce IA should include activities that also reduce school burnout. In reducing IA and SB we should include actions targeted not only on the adolescent’s group, but also on the school environment (teachers and schoolmates) and the family environment (parents and siblings).
Skin deep: The impact of tattoos and piercing on health
Authors: Peate Ian
Published: April 2020

Ian Peate explains what conditions nurses may encounter in patients with body decoration
The hopes for a cure for COVID-19
Authors: Greener Mark
Published: April 2020

The whole world is looking for a cure for COVID-19, Mark Greener looks at what progress has been made
Internal transcribed spacer (ITS) sequence-based identification of yeast biota on pomegranate surface and determination of extracellular enzyme profile
Authors: Turgut Genç Tülay , Günay Melih
Published: April 2020

Abstract. Genc TT, Günay M. 2020. Internal transcribed spacer (ITS) sequence-based identification of yeast biota on pomegranate surface and determination of extracellular enzyme profile. Nusantara Bioscience 12: 59-67. Yeasts are the most significant organisms to produce fermented products from different types of fruits such as grape, strawberry and pomegranate. The native yeasts on these fruits contribute to beverages’ quality and aroma during fermentation. Pomegranate is used in fruit juice and wine production because of high antioxidant characteristic. In order to determine yeast microbiota on the pomegranate fruits collected from Gallipoli (Gelibolu), Çanakkale-Turkey, ITS-5.8S rDNA gene region have been utilized. Also, phylogenetic relationships among identified yeast species were assigned by using sequences of ITS-5.8S rDNA gene region. In addition, extracellular enzyme activity of identified yeast strains was detected by using API-ZYM. Kluyveromyces lactis, Aureobasidium pullulans, Hanseniaspora uvarum, Candida zeylanoides, Kwoniella sp., and Metschnikowia pulcherrima and Metschnikowia ziziphicola yeast species were identified on pomegranate surface. Phylogenetic analysis, carried out in ITS-5.8S rDNA gene region of identified yeast strains, revealed the presence of five clades. Kwoniella sp., H. uvarum, M. pulcherrima, and Kl. lactis yeast strains revealed high leucine arylamidase activity. Also valine arylamidase activity was determined in M. pulcherrima and Kl. lactis yeast species. Acid phosphatase activity was determined in H. uvarum and K. lactis yeast species. Uncultured Kwoniella sp. and H. uvarum yeast species displayed high β-galactosidase and β-glucosidase activities, respectively.