bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp
Authors: Liu Wen-bin , Wang Min-meng , Dai Liu-ye , Dong Sheng-hua , Yuan Xiu-dan , Yuan Shu-li , Tang Yi , Liu Jin-hui , Peng Liang-yue , Xiao Ya-mei
Published: June 2021

Previous research has indicated that triploid crucian carp (3n fish) have preferential resistance to cadmium (Cd) compared to Carassius auratas red var. (2n fish). In this article, comparative research is further conducted between the 2n and 3n fish in terms of the immune response to Cd-induced stress. Exposure to 9 mg/L Cd for 96 h changed the hepatic function indexes remarkably in the 2n fish, but not in the 3n fish. In the serum of Cd-treated 2n fish, the levels of alanine amino transferase, aspartate aminotransferase, adenosine deaminase, and total bilirubin significantly increased, while the levels of total protein, albumin, lysozyme, and anti-superoxide anion radicals decreased demonstrating hepatotoxicity. By analysis of transcriptome profiles, many immune-related pathways were found to be involved in the response of 3n fish to the Cd-induced stress. Expression levels of the immune genes, including the interleukin genes, tumor necrosis factor super family member genes, chemokine gene, toll-like receptor gene, and inflammatory marker cyclooxygenase 2 gene were significantly enhanced in the hepatopancreas of the Cd-treated 3n fish. In contrast, the expression levels of these genes decreased in the 2n fish. This research provides a theoretical basis for polyploid fish breeding and is helpful for the ecological restoration of water due to pollution.
Phylogenomic Analyses of Hepatica Species and Comparative Analyses Within Tribe Anemoneae (Ranunculaceae)
Authors: Park Kyu Tae , Park SeonJoo
Published: June 2021

Hepatica is a small genus of Ranunculaceae with medicinal and horticultural value. We characterized nine complete chloroplast (cp) genomes of Hepatica, which ranged from 159,549 to 161,081 bp in length and had a typical quadripartite structure with a large single-copy region (LSC; 80,270–81,249 bp), a small single-copy region (SSC; 17,029–17,838 bp), and two copies of inverted repeat (IR; 31,008–31,100 bp). The cp genomes of Hepatica possess 76 protein-coding genes (PCGs), 29 tRNAs, and four rRNA genes. Comparative analyses revealed a conserved ca. 5-kb IR expansion in Hepatica and other Anemoneae; moreover, multiple inversion events occurred in Hepatica and its relatives. Analyses of selection pressure (dN/dS) showed that most of the PCGs are highly conserved except for rpl20 and rpl22 in Hepatica falconeri, Hepatica americana, and Hepatica acutiloba. Two genes (rps16 and infA) were identified as pseudogenes in Hepatica. In contrast, rpl32 gene was completely lost. The phylogenetic analyses based on 76 PCGs resolved the phylogeny of Hepatica and its related genera. Non-monophyly of Anemone s.l. indicates that Hepatica should be reclassified as an independent genus. In addition, Hepatica nobilis var. japonica is not closely related to H. nobilis.
Persistent Clones and Local Seed Recruitment Contribute to the Resilience of Enhalus acoroides Populations Under Disturbance
Authors: Dierick Jasper , Phan Thi Thuy Hang , Luong Quang Doc , Triest Ludwig
Published: June 2021

Human-induced land use in coastal areas is one of the main threats for seagrass meadows globally causing eutrophication and sedimentation. These environmental stressors induce sudden ecosystem shifts toward new alternative stable states defined by lower seagrass richness and abundance. Enhalus acoroides, a large-sized tropical seagrass species, appears to be more resistant toward environmental change compared to coexisting seagrass species. We hypothesize that reproductive strategy and the extent of seedling recruitment of E. acoroides are altered under disturbance and contribute to the persistence and resilience of E. acoroides meadows. In this research, we studied eight populations of E. acoroides in four lagoons along the South Central Coast of Vietnam using 11 polymorphic microsatellite loci. We classified land use in 6 classes based on Sentinel-2 L2A images and determined the effect of human-induced land use at different spatial scales on clonal richness and structure, fine-scale genetic structure and genetic diversity. No evidence of population size reductions due to disturbance was found, however, lagoons were strongly differentiated and may act as barriers to gene flow. The proportion and size of clones were significantly higher in populations of surrounding catchments with larger areas of agriculture, urbanization and aquaculture. We postulate that large resistant genets contribute to the resilience of E. acoroides meadows under high levels of disturbance. Although the importance of clonal growth increases with disturbance, sexual reproduction and the subsequent recruitment of seedlings remains an essential strategy for the persistence of populations of E. acoroides and should be prioritized in conservation measures to ensure broad-scale and long-term resilience toward future environmental change.
Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances
Authors: Ye Hong , Qiao Linyi , Guo Haoyu , Guo Liping , Ren Fei , Bai Jianfang , Wang Yukun
Published: June 2021

It is well known that WRKY transcription factors play essential roles in plants’ response to diverse stress responses, especially to drought and salt stresses. However, a full comprehensive analysis of this family in wheat is still missing. Here we used in silico analysis and identified 124 WRKY genes, including 294 homeologous copies from a high-quality reference genome of wheat (Triticum aestivum). We also found that the TaWRKY gene family did not undergo gene duplication rather than gene loss during the evolutionary process. The TaWRKY family members displayed different expression profiles under several abiotic stresses, indicating their unique functions in the mediation of particular responses. Furthermore, TaWRKY75-A was highly induced after polyethylene glycol and salt treatments. The ectopic expression of TaWRKY75-A in Arabidopsis enhanced drought and salt tolerance. A comparative transcriptome analysis demonstrated that TaWRKY75-A integrated jasmonic acid biosynthetic pathway and other potential metabolic pathways to increase drought and salt resistances in transgenic Arabidopsis. Our study provides valuable insights into the WRKY family in wheat and will generate a useful genetic resource for improving wheat breeding.
PagERF16 of Populus Promotes Lateral Root Proliferation and Sensitizes to Salt Stress
Authors: Wang Shengji , Huang Juanjuan , Wang Xingdou , Fan Yan , Liu Qiang , Han Youzhi
Published: June 2021

The aggravation of soil salinization limits the growth and development of plants. The AP2/ERF transcription factors (TFs) have been identified and play essential roles in plant development and stress response processes. In this study, the function of PagERF16 was detected using the overexpressing (OX) and RNAi transgenic poplar 84K hybrids. Plant growth, stomatal conductance, antioxidant enzymes activity, and PagERF16 co-expressed TFs were analyzed using morphological, physiological, and molecular methods. OX showed a more robust lateral root system with a bigger diameter and volume compared to the wild-type plants (WT). Physiological parameters indicated the bigger stomatal aperture and lower stomatal density of OX along with the lower Catalase (CAT) activity and higher malondialdehyde (MDA) content contributed to the salt sensitivity. The plant height and rooting rate of OX and RNAi were significantly worse compared to WT. Other than that, the morphology and physiology of RNAi plants were similar to WTs, suggesting that the function of PagERF16 may be redundant with other TFs. Our results indicate that when PagERF16 expression is either too high or too low, poplar growth and rooting is negatively affected. In addition, a downstream target TF, NAC45, involved in Auxin biosynthesis, was identified and PagERF16 could directly bind to its promoter to negatively regulate its expression. These results shed new light on the function of ERF TFs in plant root growth and salt stress tolerance.
A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants
Authors: Shi Gongyao , Hao Mengyuan , Tian Baoming , Cao Gangqiang , Wei Fang , Xie Zhengqing
Published: June 2021

As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.
Isosmotic Macrocation Variation Modulates Mineral Efficiency, Morpho-Physiological Traits, and Functional Properties in Hydroponically Grown Lettuce Varieties (Lactuca sativa L.)
Authors: Corrado Giandomenico , De Micco Veronica , Lucini Luigi , Miras-Moreno Begoña , Senizza Biancamaria , Zengin Gokhan , El-Nakhel Christophe , De Pascale Stefania , Rouphael Youssef
Published: June 2021

The management of mineral elements in agriculture is important for their nutritional role for plants and dietary value for humans, sparking interest in strategies that can increase mineral use efficiency and accumulation in plant food. In this work, we evaluated the effects of the isosmotic variations of the concentration on three macrocations (K, Ca, and Mg) in lettuce (Lactuca sativa L.). Our aim was to improve the nutritional components of this valuable dietary source of minerals. Using a full factorial design, we analyzed mineral utilization efficiency (UtE), leaf morphology, gas exchange parameters, phenolic profiles (through ultra-high performance liquid chromatography coupled to a quadrupole-time-of-flight (UHPLC-QTOF) mass spectrometry), and enzymatic activities in two phytochemically diverse butterhead lettuce varieties (red or green). Plants were fed in hydroponics with three nutrient solutions (NSs) with different ratios of K, Ca, and Mg. The variation of these minerals in the edible product was associated with alterations of the morphology and physiology of the leaves, and of the quality and functional properties of lettuce, with a trade-off between total accumulation and mineral UtE. Moreover, in non-limiting conditions of nutrient availability, significant mineral interactions were also present. The flexibility of the plant response to the different ratios of macrocations, and the observed large intraspecific variation, were adequate to provide mineral-specific phytochemical profiles to the edible product. Specifically, the full-red lettuce provided more interesting results in regard to the compositional and functional attributes of the leaves.
Children’s Pronoun Interpretation Problems Are Related to Theory of Mind and Inhibition, But Not Working Memory
Authors: Kuijper Sanne J. M. , Hartman Catharina A. , Hendriks Petra
Published: June 2021

In several languages, including English and Dutch, children’s acquisition of the interpretation of object pronouns (e.g., him) is delayed compared to that of reflexives (e.g., himself). Various syntactic and pragmatic explanations have been proposed to account for this delay in children’s acquisition of pronoun interpretation. This study aims to provide more insight into this delay by investigating potential cognitive mechanisms underlying this delay. Dutch-speaking children between 6 and 12 years old with autism spectrum disorder (ASD; n = 47), attention-deficit/hyperactivity disorder (ADHD; n = 36) or typical development (TD; n = 38) were tested on their interpretation and production of object pronouns and reflexives and on theory of mind, working memory, and response inhibition. It was found that all three groups of children had difficulty with pronoun interpretation and that their performance on pronoun interpretation was associated with theory of mind and inhibition. These findings support an explanation of object pronoun interpretation in terms of perspective taking, according to which listeners need to consider the speaker’s perspective in order to block coreference between the object pronoun and the subject of the same sentence. Unlike what is predicted by alternative theoretical accounts, performance on pronoun interpretation was not associated with working memory, and the children made virtually no errors in their production of object pronouns. As the difficulties with pronoun interpretation were similar for children with ASD, children with ADHD and typically developing children, this suggests that certain types of perspective taking are unaffected in children with ASD and ADHD.
Like Father Like Son: Cultural and Genetic Contributions to Song Inheritance in an Estrildid Finch
Authors: Lewis Rebecca N. , Soma Masayo , de Kort Selvino R. , Gilman R. Tucker
Published: June 2021

Social learning of vocalizations is integral to song inheritance in oscine passerines. However, other factors, such as genetic inheritance and the developmental environment, can also influence song phenotype. The relative contributions of these factors can have a strong influence on song evolution and may affect important evolutionary processes such as speciation. However, relative contributions are well-described only for a few species and are likely to vary with taxonomy. Using archived song data, we examined patterns of song inheritance in a domestic population of Java sparrows (Lonchura oryzivora), some of which had been cross-fostered. Six-hundred and seventy-six songs from 73 birds were segmented and classified into notes and note subtypes (N= 22,972), for which a range of acoustic features were measured. Overall, we found strong evidence for cultural inheritance of song structure and of the acoustic characteristics of notes; sons’ song syntax and note composition were similar to that of their social fathers and were not influenced by genetic relatedness. For vocal consistency of note subtypes, a measure of vocal performance, there was no apparent evidence of social or genetic inheritance, but both age and developmental environment influenced consistency. These findings suggest that high learning fidelity of song material, i.e., song structure and note characteristics, could allow novel variants to be preserved and accumulate over generations, with implications for evolution and conservation. However, differences in vocal performance do not show strong links to cultural inheritance, instead potentially serving as condition dependent signals.
The Nexus of the Dark Triad Personality Traits With Cyberbullying, Empathy, and Emotional Intelligence: A Structural-Equation Modeling Approach
Authors: Schade Estelle C. , Voracek Martin , Tran Ulrich S.
Published: June 2021

This study set out to elucidate the complex suite of associations between the Dark Triad personality traits (Machiavellianism, narcissism, and psychopathy), emotional intelligence, empathy, and cyberbullying, as the respective findings regarding this topic have been inconsistent. Studies preponderantly have relied on abbreviated Dark Triad measures that do not differentiate between its lower-order facets. Further, most extant studies have exclusively been based on female psychology undergraduates and have not accounted for known sex differences on the Dark Triad traits and cyberbullying, or for negative associations between cyberbullying and age. Therefore, this nexus of interrelations was investigated in a diverse community sample (N= 749). A structural equation-modeling approached was used to examine predictors of cyberbullying and to test for mediating relationships between lower-order Dark Triad facets and emotional intelligence and empathy. Multigroup models were applied to test for sex-specific patterns. Empathy did not predict cyberbullying, whereas emotional intelligence partly mediated the Dark Triad associations with cyberbullying among both sexes. Sex-specific patterns in the associations between Dark Triad traits and cyberbullying were particularly observed for the grandiose and vulnerable narcissism facets. Emotional intelligence appeared to buffer effects of grandiose narcissism on cyberbullying. Future research could fruitfully explore cyberbullies’ profiles regarding primary and secondary psychopathy, sex differences in narcissism, and buffering effects of emotional intelligence. Further improvements regarding the measurement of dark personality traits are indicated as well.
The Influence of Tertiary Education Disciplines on Self-Construals and Conflict Management Tendencies
Authors: Wee Sheila X. R. , Choo Wan Yee , Cheng Chi-Ying
Published: June 2021

While cultural difference on self-construal are well-documented, how acculturation to a new cultural environment could change an individual’s self-construal remains under-explored. In this research, how tertiary education disciplines could be associated with the endorsement of self-construals which, in turn, affect students’ conflict management tendencies were explored. Study 1 revealed that across the United States and Singapore, college students from business and social science disciplines exhibited the trend of endorsing more independent and interdependent self-construal respectively, regardless of the different dominant self-construals in the two countries. Study 2 explored how tertiary education disciplines is associated with individuals’ conflict management tendencies via the endorsement of different self-construals among Singaporeans. Findings showed that individuals from business discipline possess a more independent self-construal and in turn endorsed more of a competing conflict management style than those from social sciences. Different disciplinary cultures could link to conflict management tendencies via the endorsement of self-construals, yielding significant theoretical and practical implications.
Adaptation of the Educational Motivation Scale Into a Short Form With Multigroup Analysis in a Vocational Training and Baccalaureate Setting
Authors: Expósito-López Jorge , Romero-Díaz de la Guardia José Javier , Olmedo-Moreno Eva María , Pistón Rodríguez María Dolores , Chacón-Cuberos Ramón
Published: June 2021

The aim of the present study was to adapt the educational motivation scale into an abbreviated version (EMS-SF), in addition to analyzing its psychometric properties for use with vocational training (VT) and baccalaureate students using structural equations. A cross-sectional andex post factostudy was conducted with a sample of 1,159 students from the autonomous community of Andalusia (Spain). IBM SPSS®and IBM AMOS®software programs were used for data analysis. With regard to the main outcomes, the scale reflected good fit indices in its short form, presenting a more parsimonious and easily understood questionnaire. The questionnaire was reduced from a total of 28–19 items. In the same way, the number of dimensions was reduced from seven to four, facilitating scale understanding and interpretation according to self-determination theory. As a main finding, it was observed that the most relevant items for baccalaureate students pertained to the pleasure derived from discovering things and to overcoming challenges, whereas in VT students, items pertaining to the satisfaction generated from exerting effort, achieving one’s best and being well paid were more relevant. In conclusion, findings urge the need to strengthen intrinsic motivation in VT students with the aim of avoiding demotivation and poor academic performance.
Nature as an Ecological Asset for Positive Youth Development: Empirical Evidence From Rural Communities
Authors: Bowers Edmond P. , Larson Lincoln R. , Parry Benjamin J.
Published: June 2021

Despite increasing emphasis on youth contact with nature and associated benefits, research has not examined the direct relationship between adolescents' nature-based experiences and holistic measures of positive youth development (PYD). This gap may stem from the lack of interdisciplinary work on nature and PYD. Our study integrates multiple disciplinary perspectives to explore direct associations between time in nature, connection to nature, and the five Cs of PYD (competence, connection, confidence, character, and caring) as well as the “sixth” C of contribution. From 2015 to 2016, we collected data from 587 diverse South Carolina middle school students (57% female, 40% BIPOC) between the ages of 11 and 14 (Mage= 12.9) and living in predominantly low-income communities. OLS regression analyses indicated that across all youth, self-reported connection to nature was a consistent positive correlate of overall PYD and each of the individual Cs. Time in nature was associated with overall PYD and competence. Findings demonstrate significant associations between nature-based experiences and PYD and underscore the importance of ensuring that diverse youth can access and enjoy the developmental benefits of nature and nature-based recreation opportunities.
Financial Support or Emotional Companion: Childbearing Motivations on Children’s Development in China
Authors: Jiang Xiaodong , Cui Huaxue , Shi Tianfeng
Published: June 2021

A preference for having a son has existed among Chinese parents for centuries due to, in part, sons having to provide financial support to elderly parents, while married daughters do not have this responsibility under Confucianism. Thus, this study examined the influence of parents’ childbearing motivation (financial support or emotional companion) on children’s development (academic performance and well-being) utilizing empirical data from the 2012 China Family Panel Studies. This study included 1,541 children (aged 10–15 years) and their parents who were surveyed via a questionnaire. Using exploratory factor analysis, two dimensions of parents’ childbearing motivation were identified namely, utilitarian and psychological motivation. Furthermore, the invariance of the measurement model across the female and male group was tested. Then, results from structural equation modeling showed that parents’ childbearing motivation, particularly expected utilitarian benefits, decreased children’s expectation of the highest education, thus, worsening children’s academic performance. Alternatively, emotional/psychological motivation appeared to increase children’s self-esteem, thus, improving children’s well-being. Furthermore, gender differences were also observed. These findings have provided important insights into how childbearing motivations influence children’s development, thus, can be utilized to ensure positive development of future children in China.
Managing Grief of Bereaved Families During the COVID-19 Pandemic in Japan
Authors: Matsuda Yoko , Takebayashi Yoshitake , Nakajima Satomi , Ito Masaya
Published: June 2021

This commentary discussed the psychological issues related to bereavement in the wake of the COVID-19 pandemic. Specifically, we addressed two aspects in the context of Japanese culture. The first relates to the psychological distress of members of the bereaved family who could not visit their loved ones who had COVID-19 before or after their death. The second relates to the bereavement experience of those who were unable to be with their loved ones when the end came, even though they did not have COVID-19, because of restrictions on visiting hospitals. We seek to focus on the need for a support system for bereaved families to help them through the grieving process, and discuss end-of-life care in such circumstances, and in the post-COVID-19 era, as in current day Japan.
Comparison of the Metabolic Profiles in the Plasma and Urine Samples Between Autistic and Typically Developing Boys: A Preliminary Study
Authors: Xu Xin-Jie , Cai Xiao-E , Meng Fan-Chao , Song Tian-Jia , Wang Xiao-Xi , Wei Yi-Zhen , Zhai Fu-Jun , Long Bo , Wang Jun , You Xin , Zhang Rong
Published: June 2021

Background: Autism spectrum disorder (ASD) is defined as a pervasive developmental disorder which is caused by genetic and environmental risk factors. Besides the core behavioral symptoms, accumulated results indicate children with ASD also share some metabolic abnormalities.

Objectives: To analyze the comprehensive metabolic profiles in both of the first-morning urine and plasma samples collected from the same cohort of autistic boys.

Methods: In this study, 30 autistic boys and 30 tightly matched healthy control (HC) boys (age range: 2.4~6.7 years) were recruited. First-morning urine and plasma samples were collected and the liquid chromatography–mass spectrometry (LC-MS) was applied to obtain the untargeted metabolic profiles. The acquired data were processed by multivariate analysis and the screened metabolites were grouped by metabolic pathway.

Results: Different discriminating metabolites were found in plasma and urine samples. Notably, taurine and catechol levels were decreased in urine but increased in plasma in the same cohort of ASD children. Enriched pathway analysis revealed that perturbations in taurine and hypotaurine metabolism, phenylalanine metabolism, and arginine and proline metabolism could be found in both of the plasma and urine samples.

Conclusion: These preliminary results suggest that a series of common metabolic perturbations exist in children with ASD, and confirmed the importance to have a comprehensive analysis of the metabolites in different biological samples to reveal the full picture of the complex metabolic patterns associated with ASD. Further targeted analyses are needed to validate these results in a larger cohort.
Self-Medication Practices and Associated Factors in the Prevention and/or Treatment of COVID-19 Virus: A Population-Based Survey in Nigeria
Authors: Wegbom Anthony Ike , Edet Clement Kevin , Raimi Olatunde , Fagbamigbe Adeniyi Francis , Kiri Victor Alangibi
Published: June 2021

Background: The anxiety caused by the emergence of the novel coronavirus disease (COVID-19) globally has made many Nigerians resort to self-medication for purported protection against the disease, amid fear of contracting it from health workers and hospital environments. Therefore, this study aimed to estimate the knowledge level, causes, prevalence, and determinants of self-medication practices for the prevention and/or treatment of COVID-19 in Nigeria.

Methods: A web-based cross-sectional survey was conducted between June and July 2020 among the Nigerian population, using a self-reported questionnaire. Statistical analysis of descriptive, bivariate, and multivariate analyses was done using STATA 15.

Results: A total of 461 respondents participated in the survey. Almost all the respondents had sufficient knowledge about self-medication (96.7%). The overall prevalence of self-medication for the prevention and treatment of COVID-19 was 41%. The contributing factors were fear of stigmatization or discrimination (79.5%), fear of being quarantine (77.3%), and fear of infection or contact with a suspected person (76.3%). The proximal reasons for self-medication were emergency illness (49.1%), delays in receiving hospital services (28.1%), distance to the health facility (23%), and proximity of the pharmacy (21%). The most commonly used drugs for self-medication were vitamin C and multivitamin (51.8%) and antimalarials (24.9%). These drugs were bought mainly from pharmacies (73.9%). From the multivariable logistic regression model, males (OR: 0.79; 95% CI: 0.07–0.54), and sufficient knowledge on SM (OR: 0.64; 95% CI: 0.19–0.77) were significantly associated with self-medication.

Conclusion: The key finding of this study was the use of different over-the-counter medications for the prevention (mainly vitamin C and multivitamins) and treatment (antibiotics/antimicrobial) of perceived COVID-19 infection by Nigerians with mainly tertiary education. This is despite their high knowledge and risk associated with self-medication. We suggest that medication outlets, media and community should be engaged to support the rational use of medication.
Outdoor Physical Activity During the First Wave of the COVID-19 Pandemic. A Comparative Analysis of Government Restrictions in Italy, France, and Germany
Authors: Michelini Enrico , Bortoletto Nico , Porrovecchio Alessandro
Published: June 2021

Introduction: Mandated restrictions on outdoor physical activity (PA) during the coronavirus pandemic disrupted the lifeworld of millions of people and led to a contradictory situation. On the one hand, PA was perceived as risky behaviour, as it might facilitate transmission of the virus. On the other hand, while taking precautions, regular PA was an important tool to promote the population's health during the lockdown.

Methods: This paper examines the differences in government restrictions on PA in France, Germany, and Italy during the first wave of the COVID-19 pandemic. We draw on techniques of qualitative content analysis and apply a critical theoretical framework to assess the countries' restrictions on PA.

Results: Our analysis shows that the restrictions on PA varied in the three countries, in all three countries. This variance is attributed both to differences in the timing and severity of the pandemic in the countries analysed, as well as to the divergence in the relationships between the countries' sport and health systems.

Conclusion: At the national level, the variance in restrictions on PA reflect the differences in the spread of the coronavirus and in the health systems' understanding of and approach to PA. The global scientific discourse on the pandemic represents a further key influencing factor. The management of the coronavirus pandemic has demonstrated that the extreme complexity of societies in terms of public health, politics, and the economy pose challenges and unsolvable contradictions.
Application of Discrete Choice Experiment in Health Care: A Bibliometric Analysis
Authors: Wang Yue , Wang Zhangyi , Wang Zhao , Li Xuechun , Pang Xiaoli , Wang Shuling
Published: June 2021

Background: Discrete choice experiment (DCE) as a tool that can measure medical stakeholders' preferences especially patients recently has been increasingly applied in health care.

Objective: The aim of this study was to examine the hotspots and trends of the application of DCE in health care and to provide reference and direction for further development of DCE in the future.

Method: A bibliometric method was implemented using the Web of Science (WoS) Core Collection for the period from the database established to December 8, 2020. The data files are imported into CiteSpace and Excel to analyze and visualize the annual volume of productive, authors, countries, cited journals, cited articles, and keywords.

Results: A total of 1,811 articles were retrieved, then we read the abstract of each paper one by one, and 1,562 articles were included after screening, with an exponential increase in publication volume. John F. P. Bridges contributed to 40 publications and ranked first, followed by F. Reed Johnson (n = 37), Julie Ratcliffe (n = 36). The majority of the papers were conducted in the United States (n = 513) and the United Kingdom (n = 433). The top three cited journals were “Health Economics” (n = 981), “Value in Health” (n = 893), and “Pharmaceutical Economics” (n = 774), and the top three articles were “Constructing experimental designs for discrete-choice experiments: report of the ISPOR Conjoint Analysis Experimental Design Good Research Practices Task Force,” “Conjoint analysis applications in health-a checklist: a report of the ISPOR Good Research Practices for Conjoint Analysis Task Force,” and “Discrete choice experiments in health economics: a review of the literature.” The research hotspots and trends included “health technology assessment,” “survival,” “preference based measure,” and “health state valuation.”

Conclusion: The size of the literature about DCE studies in health care showed a noticeable increase in the past decade. The application of DCE in health care remains in an early growth phase, and “health technology assessment,” “survival,” “preference based measure,” and “health state valuation” reflected the latest research hotpots and future trends.
Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars
Authors: Vital Nádia , Antunes Susana , Louro Henriqueta , Vaz Fátima , Simões Tânia , Penque Deborah , Silva Maria João
Published: June 2021

Environmental tobacco smoke (ETS) has been recognized as a major health hazard by environmental and public health authorities worldwide. In Portugal, smoke-free laws are in force for some years, banning smoking in most indoor public spaces. However, in hospitality venues such as restaurants and bars, owners can still choose between a total smoke-free policy or a partial smoking restriction with designated smoking areas, if adequate reinforced ventilation systems are implemented. Despite that, a previous study showed that workers remained continuously exposed to higher ETS pollution in Lisbon restaurants and bars where smoking was still allowed, comparatively to total smoke-free venues. This was assessed by measurements of indoor PM2.5 and urinary cotinine, a biomarkers of tobacco smoke exposure, demonstrating that partial smoking restrictions do not effectively protect workers from ETS. The aim of the present work was to characterize effect and susceptibility biomarkers in non-smokers from those hospitality venues occupationally exposed to ETS comparatively to non-exposed ones. A group of smokers was also included for comparison. The sister chromatid exchange (SCE), micronucleus (MN) and comet assays in whole peripheral blood lymphocytes (PBLs) and the micronucleus assay in exfoliated buccal cells, were used as biomarkers of genotoxicity. Furthermore, a comet assay after ex vivo challenge of leukocytes with an alkylating agent, ethyl methanesulfonate (EMS), was used to analyze the repair capacity of those cells. Genetic polymorphisms in genes associated with metabolism and DNA repair were also included. The results showed no clear association between occupational exposure to ETS and the induction of genotoxicity. Interestingly, the leukocytes from non-smoking ETS-exposed individuals displayed lower DNA damage levels in response to the ex vivo EMS challenge, in comparison to those from non-exposed workers, suggesting a possible adaptive response. The contribution of individual susceptibility to the effect biomarkers studied was unclear, deserving further investigation.
Transfer Learning–Based Artificial Neural Networks Post-Equalizers for Underwater Visible Light Communication
Authors: Zhao Yiheng , Yu Shaohua , Chi Nan
Published: June 2021

In this article, we demonstrate two transfer learning–based dual-branch multilayer perceptron post-equalizers (TL-DBMLPs) in carrierless amplitude and phase (CAP) modulation-based underwater visible light communication (UVLC) system. The transfer learning algorithm could reduce the dependence of artificial neural networks (ANN)–based post-equalizer on big data and extended training cycles. Compared with DBMLP, the TL-DBMLP is more robust to the jitter of the bias current (Ibias) of light-emitting diode (LED), which indicates that TL-DBMLP does not require further training in Ibias varying UVLC system. In terms of voltage peak-to-peak (Vpp) varying VLC system, DBMLP requires a training set with a size of more than 105 and 50 training epochs, which quantitatively prove the effectiveness of DBMLP in reducing reliance on large amount of training epochs. On the counterpart, the TL-DBMLP only requires a training set with a size of less than 2×104 and 10 training epochs, which quantitatively prove the effectiveness of DBMLP in reducing reliance on big data. Finally, we experimentally demonstrate that transfer learning can effectively reduce ANN dependence on extensive size training data and large amount of training epochs, whether in VLC systems with varying Ibias and varying Vpp.
Impact of COVID-19 Pandemic on Quality of Life of Type II Diabetes Patients With Periodontitis
Authors: Morales Alicia , Corral-Nuñez Camila , Galaz Carolina , Henríquez Leslie , Mery María , Mesa Cesar , Strauss Franz , Cavalla Franco , Baeza Mauricio , Valenzuela-Villarroel Francisca , Gamonal Jorge
Published: June 2021

Background: Confinement due to the COVID-19 pandemic has made dental treatments impossible in Chile and many other countries, including diabetic patients with periodontitis. The aim of the present study was to evaluate the impact of periodontal therapy in terms of oral health-related quality of life (OHRQoL) during the COVID-19 pandemic in a cohort of diabetic patients with periodontitis.

Material and Methods: Thirty-eight diabetic patients with stage III-IV periodontitis, enrolled for periodontal therapy, were screened. Periodontal clinical parameters including clinical attachment loss (CAL), probing pocket depth (PPD) and bleeding on probing (BOP) as well as glycated hemoglobin (HbA1c) were evaluated at baseline and 3 months follow-up prior the pandemic. The OHRQoL changes by means of Oral Health Impact Profile (OHIP-14) and a self-reported oral health questionnaire were assessed at baseline (prior pandemic) and during the pandemic via telemonitoring.

Results: Thirty-one patients received non-surgical periodontal therapy prior to the pandemic. Out of the 31 patients, four died due to COVID-19 resulting in 27 patients available for telemonitoring at the time of the pandemic. Periodontal therapy significantly improved CAL, PPD and BOP (p < 0.05) but not HbA1c (p > 0.05) between baseline and 3 months follow-up pior to the pandemic. Total OHIP-14 scores significantly improved between baseline and the middle of pandemic (intragroup comparison p = 0.00411). In particular, OHIP-14 scores related to the “Physical pain” (intragroup comparison p = 0.04) and “Psychological disability” (intragroup comparison p = 0.00) significantly improved between baseline and the middle of pandemic.

Conclusions: In diabetic type II patients with periodontitis periodontal therapy tends to improve the oral health-related quality of life despite the COVID-19 pandemic.
Decision Support System Applications for Scheduling in Professional Team Sport. The Team's Perspective
Authors: Schelling Xavier , Fernández Jose , Ward Patrick , Fernández Javier , Robertson Sam
Published: June 2021

Background: Periodization implies the systematic planning of training and competition with the goal of reaching the best possible performance in the most important competition. In team sports, this consists of finding a flight-and-practice schedule that maximizes the opportunities to perform the periodized contents (e.g., trips, practices, games, and days off). This process is conducted whilst considering known constraints (e.g., competitive schedule, roster availability, weather, especial events, holidays, or emotional effect of days away). The way a scheduling decision support system (DSS) leads users to make a decision should allow for flexibility, whilst minimizing users' confusion and facilitating the understanding of the recommendation given by the scheduling decision support system. Traditional approaches to solving scheduling problems use either simulation models, analytical models, heuristic approaches or a combination of these methods. When it comes to evaluate how the scheduling DSS is performing, three overarching aspects need to be reviewed: context satisfaction, process efficiency, and output quality. Appropriate training periodization and scheduling of trips and training sessions are critical for teams to optimize training and recovery processes in order to maximize health and performance. This article presents a methodological framework for designing decision-support systems for scheduling in professional team sports.
Fasciola hepatica Control Practices on a Sample of Dairy Farms in Victoria, Australia
Authors: Kelley Jane M. , Rawlin Grant , Beddoe Travis , Stevenson Mark , Spithill Terry W.
Published: June 2021

In Australia, little is known about the strategies used by farmers to control Fasciola hepatica (F. hepatica) infection in dairy cattle. Triclabendazole-resistant F. hepatica have recently been found on several dairy and beef properties in Australia. It is difficult to draw conclusions about how widespread resistance is in Australian dairy cattle because we have little information about flukicide usage, drug resistance testing, and alternative flukicide usage on-farm. The study objectives were to determine how dairy farmers are currently controlling F. hepatica and to identify knowledge gaps where F. hepatica control strategies need to be communicated to farmers to improve management. The survey was distributed online or by hard copy and 36 dairy farmers completed the survey. There were 34 questions including closed, open-ended, multicheck box, demographic, and text questions. Descriptive statistics were used to quantify each response. The survey results showed high use of clorsulon, limited rotation of flukicides, and limited use of diagnostic tests to inform treatment options and timing. There was poor adherence to best management practice in determining the dose of flukicides administered to cattle, with farmers often relying on estimating body weights or average body weights, suggesting that underdosing of animals is likely to be prevalent. Most respondents in this study did not isolate and quarantine treated and newly returned or purchased animals before joining them with the main herd. The research identified four knowledge gaps where communication needs to be enhanced to improve control of F. hepatica: diagnostic testing to inform flukicide use, rotation of flukicide actives, flukicide administration, and increased testing of replacement animals.
Effect of Cooking Techniques on the in vitro Protein Digestibility, Fatty Acid Profile, and Oxidative Status of Mealworms (Tenebrio molitor)
Authors: Mancini Simone , Mattioli Simona , Paolucci Simone , Fratini Filippo , Dal Bosco Alessandro , Tuccinardi Tiziano , Paci Gisella
Published: June 2021

Tenebrio molitor (T. molitor) (mealworm) larvae are one of the most promising insects for feed–food purposes. Mealworms are rich in several macro and micro nutritional elements and can be practically reared on side stream substrates. In this study, the effects of seven different cooking techniques were tested on the nutritional value of mealworms focusing the attention on protein digestibility, fatty acid (FA) profile, and oxidative status. Uncooked larvae (UC) were used as control and compared to two combinations of temperature/time in oven cooking (70°C for 30 min, OC70-30, 150°C for 10 min, OC150-10), two methods of frying (mealworms fried in sunflower oil as deep fry, DF, or pan fry, PF), microwaving (MW), boiling (in plastic bag under vacuum, BO), and steaming (ST). Proximate composition, in vitro digestibility (gastric and duodenal), FA profile, and oxidative status (tocopherol and tocotrienol, carbonyl, and lipid oxidation) were then tested. Cooking technique affected all the tested parameters. As expected, cooking affected proximate composition in relation to the method applied (dry matter increased after oven cooking and frying; lipids increased by frying). In vitro digestion revealed the highest value for the OC70-30 method, followed by UC and ST. Deep frying revealed the worst digestibility percentage. FA profile was deeply affected by the cooking technique, with general decrease in SFA and MUFA. The highest modifications in FA profile were revealed in ST larvae with an increased percentage of linoleic acid linked to the lowering of SFA and MUFA contents. Furthermore, deep frying larvae in sunflower oil increased the relative abundance of PUFAs. Tocols values were higher in DF and MW groups than PF (about 6-fold more) and all other groups (7-fold more). Carbonyls increased with oven cooking (OC150-10 and OC70-30), whereas the values were lower with frying and similar to ST and UC. Lipid oxidation was highest as well in OC150-10 but similar to frying methods (DF and PF). Based on the obtained results, it can be concluded that mealworm larvae surely meet human nutritional requirements, but the cooking method must be carefully chosen to maintain a high nutritional value.
Morphological and Physiological Root Traits and Their Relationship with Nitrogen Uptake in Wheat Varieties Released from 1915 to 2013
Authors: Puccio Guglielmo , Ingraffia Rosolino , Giambalvo Dario , Amato Gaetano , Frenda Alfonso S.
Published: June 2021

Identifying genotypes with a greater ability to absorb nitrogen (N) may be important to reducing N loss in the environment and improving the sustainability of agricultural systems. This study extends the knowledge of variability among wheat genotypes in terms of morphological or physiological root traits, N uptake under conditions of low soil N availability, and in the amount and rapidity of the use of N supplied with fertilizer. Nine genotypes of durum wheat were chosen for their different morpho-phenological characteristics and year of their release. The isotopic tracer 15N was used to measure the fertilizer N uptake efficiency. The results show that durum wheat breeding did not have univocal effects on the characteristics of the root system (weight, length, specific root length, etc.) or N uptake capacity. The differences in N uptake among the studied genotypes when grown in conditions of low N availability appear to be related more to differences in uptake efficiency per unit of weight and length of the root system than to differences in the morphological root traits. The differences among the genotypes in the speed and the ability to take advantage of the greater N availability, determined by N fertilization, appear to a certain extent to be related to the development of the root system and the photosynthesizing area. This study highlights some variability within the species in terms of the development, distribution, and efficiency of the root system, which suggests that there may be sufficient grounds for improving these traits with positive effects in terms of adaptability to difficult environments and resilience to climate change.
Swine Inflammation and Necrosis Syndrome (SINS)
Authors: Reiner Gerald , Kuehling Josef , Loewenstein Frederik , Lechner Mirjam , Becker Sabrina
Published: June 2021

Tail biting is a prevalent and undesirable behaviour in pigs and a major source of significant reduction in well-being. However, focusing on biting considers only one part of the solution, because tail damage can be found with a high prevalence without any action by other pigs. The lesions are not limited to the tail but can also be found in the ears, heels, soles, claw coronary bands, teats, navel, vulva, and face. Environmental improvement alone often fails to overcome the problem. This review addresses a new inflammation and necrosis syndrome in swine (SINS). It shows the clinical signs and the frequencies of occurrence in different age groups. It compiles scientific evidence from clinical and histopathological studies in newborn piglets that argue for a primary endogenous aetiology of the disease. Bringing together the findings of a broad body of research, the possible mechanisms leading to the disease are identified and then discussed. This part will especially focus on microbe-associated molecular patterns in the circulation and their role in activating defence mechanisms and inflammation. Finally, the methods are identified to ameliorate the problem by optimizing husbandry and selecting a suitable breeding stock.