bluuebunny/crossref_metadata_2025 huggingface dataset:
A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence
Authors: Hirakawa Hidetada , Suzue Kazutomo , Uchida Motoyuki , Takita Ayako , Kamitani Wataru , Tomita Haruyoshi
Published: May 2022

Enterohemorrhagic Escherichia coli (EHEC) is one of the most common foodborne pathogens. However, no drug that prevents the severe complications caused by this bacterium has been approved yet. This study showed that a macroporous magnesium oxide (MgO)-templated carbon material (MgOC150) adsorbs Shiga toxins, and Type III secretory EspA/EspB proteins responsible for EHEC pathogenesis, and decreases the extracellular levels of these proteins. On the other hand, this material did not affect the growth of EHEC. Citrobacter rodentium traditionally used to estimate Type III secretion system-associated virulence in mice is highly virulent. The survival period of infected mice was prolonged when MgOC150 was administered. This adsorbent disturbed neither mammalian cells nor normal intestinal bacteria, such as Enterococcus hirae, Lactobacillus acidophilus, and Lactobacillus casei. In contrast, MgOC150 adsorbed antimicrobial agents, including β-lactams, quinolones, tetracyclines, and trimethoprim/sulfamethoxazole. However, fosfomycin and amikacin were not adsorbed. Thus, MgOC150 can be used with fosfomycin and amikacin to treat infections. MgOC150 is used for industrial purposes, such as an electrode catalyst, a bioelectrode, and enzyme immobilization. The study proposed another potential application of MgOC150, assisting anti-EHEC chemotherapy.
SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation
Authors: Cheng Chuansong , Yan Xiaotong , Liu Binxiong , Jiang Tao , Zhou Ziwen , Guo Fengting , Zhang Qianwen , Li Changcheng , Fang Ting
Published: May 2022

Cronobacter sakazakii is a common foodborne pathogen, and the mortality rate of its infection is as high as 40–80%. SdiA acts as a quorum sensing regulator in many foodborne pathogens, but its role in C. sakazakii remains unclear. Here, we further determined the effect of the sdiA gene in C. sakazakii pathogenicity. The SdiA gene in C. sakazakii was knocked out by gene editing technology, and the biological characteristics of the ΔsdiA mutant of C. sakazakii were studied, followed by transcriptome analysis to elucidate its effects. The results suggested that SdiA gene enhanced the drug resistance of C. sakazakii but diminished its motility, adhesion and biofilm formation ability and had no effect on its growth. Transcriptome analysis showed that the ΔsdiA upregulated the expression levels of D-galactose operon genes (including dgoR, dgoK, dgoA, dgoD and dgoT) and flagella-related genes (FliA and FliC) in C. sakazakii and downregulated the expression levels of related genes in the type VI secretion system (VasK gene was downregulated by 1.53-fold) and ABC transport system (downregulated by 1.5-fold), indicating that SdiA gene was related to the physiological metabolism of C. sakazakii. The results were useful for clarifying the pathogenic mechanism of C. sakazakii and provide a theoretical basis for controlling bacterial infection.
Engineered Schwann Cell-Based Therapies for Injury Peripheral Nerve Reconstruction
Authors: Su Qisong , Nasser Moussa Ide , He Jiaming , Deng Gang , Ouyang Qing , Zhuang Donglin , Deng Yuzhi , Hu Haoyun , Liu Nanbo , Li Zhetao , Zhu Ping , Li Ge
Published: May 2022

Compared with the central nervous system, the adult peripheral nervous system possesses a remarkable regenerative capacity, which is due to the strong plasticity of Schwann cells (SCs) in peripheral nerves. After peripheral nervous injury, SCs de-differentiate and transform into repair phenotypes, and play a critical role in axonal regeneration, myelin formation, and clearance of axonal and myelin debris. In view of the limited self-repair capability of SCs for long segment defects of peripheral nerve defects, it is of great clinical value to supplement SCs in necrotic areas through gene modification or stem cell transplantation or to construct tissue-engineered nerve combined with bioactive scaffolds to repair such tissue defects. Based on the developmental lineage of SCs and the gene regulation network after peripheral nerve injury (PNI), this review summarizes the possibility of using SCs constructed by the latest gene modification technology to repair PNI. The therapeutic effects of tissue-engineered nerve constructed by materials combined with Schwann cells resembles autologous transplantation, which is the gold standard for PNI repair. Therefore, this review generalizes the research progress of biomaterials combined with Schwann cells for PNI repair. Based on the difficulty of donor sources, this review also discusses the potential of “unlimited” provision of pluripotent stem cells capable of directing differentiation or transforming existing somatic cells into induced SCs. The summary of these concepts and therapeutic strategies makes it possible for SCs to be used more effectively in the repair of PNI.
Cross-Frequency Slow Oscillation–Spindle Coupling in a Biophysically Realistic Thalamocortical Neural Mass Model
Authors: Jajcay Nikola , Cakan Caglar , Obermayer Klaus
Published: May 2022

Sleep manifests itself by the spontaneous emergence of characteristic oscillatory rhythms, which often time-lock and are implicated in memory formation. Here, we analyze a neural mass model of the thalamocortical loop in which the cortical node can generate slow oscillations (approximately 1 Hz) while its thalamic component can generate fast sleep spindles of σ-band activity (12–15 Hz). We study the dynamics for different coupling strengths between the thalamic and cortical nodes, for different conductance values of the thalamic node's potassium leak and hyperpolarization-activated cation-nonselective currents, and for different parameter regimes of the cortical node. The latter are listed as follows: (1) a low activity (DOWN) state with noise-induced, transient excursions into a high activity (UP) state, (2) an adaptation induced slow oscillation limit cycle with alternating UP and DOWN states, and (3) a high activity (UP) state with noise-induced, transient excursions into the low activity (DOWN) state. During UP states, thalamic spindling is abolished or reduced. During DOWN states, the thalamic node generates sleep spindles, which in turn can cause DOWN to UP transitions in the cortical node. Consequently, this leads to spindle-induced UP state transitions in parameter regime (1), thalamic spindles induced in some but not all DOWN states in regime (2), and thalamic spindles following UP to DOWN transitions in regime (3). The spindle-induced σ-band activity in the cortical node, however, is typically the strongest during the UP state, which follows a DOWN state “window of opportunity” for spindling. When the cortical node is parametrized in regime (3), the model well explains the interactions between slow oscillations and sleep spindles observed experimentally during Non-Rapid Eye Movement sleep. The model is computationally efficient and can be integrated into large-scale modeling frameworks to study spatial aspects like sleep wave propagation.
Integrating Chronic Obstructive Pulmonary Disease Treatment With 8-Week Tai Chi Chuan Practice: An Exploration of Mind-Body Intervention and Neural Mechanism
Authors: Shen Haoran , Chen Li-Zhen , Hu Zhuoer , Yao Xiaoyan , Yang Tao , Zhang Lan , Tu Qiang , Li Guangxi , Wei Gao-Xia
Published: May 2022

Objective

This study aims to explore the effect of integrating routine treatment with Tai Chi Chuan (TCC) intervention on the clinical symptom of patients with Chronic Obstructive Pulmonary Disease (COPD) from clinical and neurological perspectives.

Methods

Twenty patients with COPD were recruited for regular treatment combined with 8-week TCC rehabilitative practice. Clinical symptoms were evaluated by Chronic Obstructive Pulmonary Symptom Assessment Scale (CAT) and Modified Dyspnea Scale (mMRC) at baseline and after treatment. Resting-state MRI scan was also performed with multiline T2-weighted echo-planar imaging (EPI) to acquire their functional images before and after the treatment. TCC rehabilitation involved a total of 8 weeks of practice with 90 min per session, three times a week.

Results

After an 8-week integration routine treatment with TCC practice, the patient’s clinical symptoms improved significantly. Imaging analysis showed that COPD patients exhibited decreased Degree of Centrality (DC) in the right inferior frontal gyrus (IFG), right middle frontal gyrus, bilateral cingulate cortex, bilateral precuneus, and right precentral gyrus. Moreover, correlation analysis found that the decreased DC in the right IFG was positively correlated with the CAT improvements.

Conclusion

The routine treatment involving TCC rehabilitation practice could improve the clinical symptoms of patients with COPD. The right IFG might be a key brain region to contribute to the neural mechanism underlying integrative intervention on the clinical symptoms in COPD. These findings provide neurological evidence for treating COPD rehabilitation practice with mind-body practice based on Chinese culture to some extent, which also advances the understanding of the efficacy of TCC as the adjuvant technology from a neuroscience perspective.

Clinical Trial Registration:

[http://www.chictr.org.cn/showproj.aspx?proj=45189], identifier [ChiCTR1900028335].
Current and Future Approaches for Diagnosing Small Intestinal Dysbiosis in Patients With Symptoms of Functional Dyspepsia
Authors: Shah Ayesha , Talley Nicholas J. , Holtmann Gerald
Published: May 2022

The development and application of next generation sequencing technologies for clinical gastroenterology research has provided evidence that microbial dysbiosis is of relevance for the pathogenesis of gastrointestinal and extra-intestinal diseases. Microbial dysbiosis is characterized as alterations of diversity, function, and density of the intestinal microbes. Emerging evidence suggests that alterations of the gastrointestinal microbiome are important for the pathophysiology of a variety of functional gastrointestinal conditions, e.g., irritable bowel syndrome (IBS) and functional dyspepsia (FD), also known as disorders of brain-gut axis interaction. Clinicians have for many years recognized that small intestinal bacterial overgrowth (SIBO) is typified by a microbial dysbiosis that is underpinned by abnormal bacterial loads in these sites. SIBO presents with symptoms which overlap with symptoms of FD and IBS, point toward the possibility that SIBO is either the cause or the consequence of functional gastrointestinal disorders (FGIDs). More recently, new terms including “intestinal methanogen overgrowth” and “small intestinal fungal overgrowth” have been introduced to emphasize the contribution of methane production by archea and fungi in small intestinal dysbiosis. There is emerging data that targeted antimicrobial treatment of SIBO in patients with FD who simultaneously may or may not have IBS, results in symptom improvement and normalization of positive breath tests. However, the association between SIBO and FGIDs remains controversial, since widely accepted diagnostic tests for SIBO are lacking. Culture of jejunal fluid aspirate has been proposed as the “traditional gold standard” for establishing the diagnosis of SIBO. Utilizing jejunal fluid culture, the results can potentially be affected by cross contamination from oropharyngeal and luminal microbes, and there is controversy regarding the best cut off values for SIBO diagnosis. Thus, it is rarely used in routine clinical settings. These limitations have led to the development of breath tests, which when compared with the “traditional gold standard,” have sub-optimal sensitivity and specificity for SIBO diagnosis. With newer diagnostic approaches–based upon applications of the molecular techniques there is an opportunity to characterize the duodenal and colonic mucosa associated microbiome and associated gut microbiota dysbiosis in patients with various gastrointestinal and extraintestinal diseases. Furthermore, the role of confounders like psychological co-morbidities, medications, dietary practices, and environmental factors on the gastrointestinal microbiome in health and disease also needs to be explored.
Neurophysiological Effects of the Anterior Cingulate Cortex on the Exacerbation of Crohn’s Disease: A Combined fMRI-MRS Study
Authors: Kong Ning , Gao Chen , Zhang Fan , Zhang Meng , Yue Juan , Lv Kun , Zhang Qi , Fan Yihong , Lv Bin , Zang Yufeng , Xu Maosheng
Published: May 2022

Background

Crohn’s disease (CD) is characterized by repetitive phases of remission and exacerbation, the quality of life of patients with CD is strongly influenced by disease activity, as patients in the active phase experience significantly worse symptoms. To investigate the underlying mechanism of how the course of CD is exacerbated based on the bi-directionality of the brain-gut axis (BGA), we conducted a multi-modality neuroimaging study that combined resting-state functional magnetic resonance imaging (rs-fMRI) with proton magnetic resonance spectroscopy (MRS) to detect abnormalities in the anterior cingulate cortex (ACC).

Materials and Methods

Clinical scales including Visual Analog Scale (VAS) and Hospital Anxiety and Depression Scale (HADS) were used to evaluate the degree of abdominal pain and mood state of participants. We made a comparison between CD patients in the active phase, the remission phase and healthy controls (HCs), not only employed the innovative wavelet-transform to analyze the amplitude of low frequency fluctuation (ALFF) but also compared the sensitivity of wavelet-transform and the traditional fast Fourier transform (FFT). Brain metabolites such as glutamate (Glu), myo-inositol (mIns) and gamma-aminobutyric acid (GABA) were also detected. Then correlation analysis was made to see whether changes in the ACC correlated with CD’s clinical symptoms.

Results

CD patients in the active phase showed higher VAS scores (p = 0.025), the scores of anxiety and depression were also higher (all p < 0.05). Wavelet-transform is slightly more sensitive in the current research. Patients in the active phase exhibited higher ALFF in the left ACC and the left superior frontal gyrus, medial (SFGmed). Patients in the active phase showed increased Glu levels in the ACC than patients in the remission phase or HCs (p = 0.039 and 0.034 respectively) and lower levels of mIns than HCs (p = 0.036). There was a positive correlation between mWavelet-ALFF values of the ACC and HADS-depression scores in CD patients (r = 0.462, p = 0.006). Besides, concentrations of Glu positively correlated with mWavelet-ALFF in the ACC in all participants (r = 0.367, p = 0.006).

Conclusion

Abnormal spontaneous activity and metabolic levels in the ACC were detected in CD patients in the active phase along with severer abdominal pain and worse mood state, these may contribute to the exacerbation of CD. Therefore, the ACC might be a potential neural alternative for managing the exacerbation of CD.
Leukocytes and Neutrophil–Lymphocyte Ratio as Indicators of Insulin Resistance in Overweight/Obese School-Children
Authors: Rodríguez-Rodríguez Elena , Salas-González M. Dolores , Ortega Rosa M. , López-Sobaler Ana M.
Published: May 2022

Background and Aims

Inflammation in overweight/obesity may condition the development of insulin resistance (IR). New markers of inflammation and systemic inflammation, such as leukocyte and platelet counts, the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and monocyte-to-lymphocyte ratio (MLR), could be used as indicators of IR. The aim of the present study was to investigate the relationship between these markers and IR in overweight/obese children.

Materials and Methods

A total of 1,035 schoolchildren were studied (398 overweight/obese). Anthropometric, hematological and biochemical measurements were collected. Inflammation was considered to be present when the values of leukocytes, platelets, NLR, PLR and MLR were ≥p75 for each sex. IR was defined as HOMA-IR >3.16.

Results

In overweight/obese schoolchildren, leukocytes were higher in both boys and girls, and platelets and NLR were higher in boys with IR than in boys with insulin sensitivity. An association between leucocytes and NLR values (≥p75) and IR was found in the entire population [2.911 (1.328–6.381); p = 0.008 and 2.660 (1.185–5.968); p = 0.018, respectively] and in boys [9.255 (1.896–45.168); p = 0.006 and 6.996 (1.377–35.544); p = 0.019, respectively].

Conclusion

Elevated white blood cell values and elevated NLR in overweight/obese children, and particularly in boys, could be indicators of the presence of IR in these subjects and should be considered when predicting possible complications, such as type II diabetes mellitus.
Evaluation of Mutton Quality Characteristics of Dongxiang Tribute Sheep Based on Membership Function and Gas Chromatography and Ion Mobility Spectrometry
Authors: Lu Zengkui , Li Jianye , Yuan Chao , Xi Bin , Yang Bohui , Meng Xianyu , Guo Tingting , Yue Yaojing , Gao Yaqin , Liu Jianbin , Sun Xiaoping
Published: May 2022

Dongxiang tribute sheep have a history of use in food dishes such as “Dongxiang Handgrip,” which dates back hundreds of years and is a favorite halal food in northwestern China. However, little is known about the mutton quality characteristics of Dongxiang tribute sheep. Here, we measured the sensory characteristics, nutritional quality, and flavor substances to comprehensively evaluate the mutton quality characteristics of these sheep. The mutton qualities of Dongxiang tribute, Tibetan, Ujumqin, and Hu sheep were comprehensively evaluated by membership function. Subsequently, the volatile components in mutton samples from 30 Dongxiang tribute sheep were detected via gas chromatography and ion mobility spectrometry (GC-IMS), and their fingerprints were established. The result of meat quality revealed that the shear force, the contents of protein, essential amino acid (EAA), non-essential amino acid (NEAA), and n-6/n-3 ratio of Dongxiang tribute mutton were better than the other three breeds. Membership functions were calculated for 10 physical and chemical indexes of mutton quality, and the comprehensive membership function values of the four breeds in order of highest to lowest mutton quality were Tibetan sheep (0.76) > Dongxiang tribute sheep (0.49) > Hu sheep (0.46) > Ujumqin sheep (0.33). Thirty volatile compounds were identified via GC-IMS: seven alcohols, eight aldehydes, five ketones, two esters, two phenols, one ether, one furan, one acid, two hydrocarbons, and one pyrazine. Ketones, aldehydes, and alcohols were the main volatile compounds forming the flavor of Dongxiang tribute sheep mutton. The reliability of the results was validated by PCA (principal component analysis) and similarity analyses. Our results provide reference value for consumers of mutton in China.
Perspective: Soybeans Can Help Address the Caloric and Protein Needs of a Growing Global Population
Authors: Messina Mark
Published: May 2022

Feeding a growing global population with projected rising socioeconomic status will require additional sources of calories and especially protein. These sources need to align with the Sustainable Development Goals established by the Food and Agriculture Organization of the United Nations. The soybean is uniquely positioned to meet this challenge based on the following criteria: (1) Global soybean production exceeds by ~4 times the production of all pulses combined (2) Soybeans are higher in protein than other legumes and soy protein quality is similar to animal protein quality (3) Soybeans are an excellent source of healthy fat, including both essential fatty acids (4) Soybeans, like other legumes, symbiotically fix atmospheric nitrogen thereby reducing the need for fertilizer inputs (5) Greenhouse gas emissions per unit protein are lower than for nearly all other foods (6) Soybeans, like other legumes, are also recognized as an affordable food that can be incorporated into diverse diets regardless of economic standing and (7) The range of foods produced from soybeans constitutes an important position in historic and contemporary cuisines, cultures and emerging consumer trends for plant-based protein. Although most soybeans are currently used for animal feed, soybean use is dictated by consumer demand. Therefore, soybeans are well positioned to meet future global needs for energy and protein. Armed with this knowledge, health professionals can feel justified in encouraging greater consumption of soyfoods for both personal and planetary reasons.
Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis
Authors: Swaminathan Jyothishmathi , Maegawa Shinji , Shaik Shavali , Sharma Ajay , Bravo-Alegria Javiera , Guo Lei , Xu Lin , Harmanci Arif , Gopalakrishnan Vidya
Published: May 2022

The RE1 Silencing Transcription Factor (REST) is a major regulator of neurogenesis and brain development. Medulloblastoma (MB) is a pediatric brain cancer characterized by a blockade of neuronal specification. REST gene expression is aberrantly elevated in a subset of MBs that are driven by constitutive activation of sonic hedgehog (SHH) signaling in cerebellar granular progenitor cells (CGNPs), the cells of origin of this subgroup of tumors. To understand its transcriptional deregulation in MBs, we first studied control of Rest gene expression during neuronal differentiation of normal mouse CGNPs. Higher Rest expression was observed in proliferating CGNPs compared to differentiating neurons. Interestingly, two Rest isoforms were expressed in CGNPs, of which only one showed a significant reduction in expression during neurogenesis. In proliferating CGNPs, higher MLL4 and KDM7A activities opposed by the repressive polycomb repressive complex 2 (PRC2) and the G9A/G9A-like protein (GLP) complex function allowed Rest homeostasis. During differentiation, reduction in MLL4 enrichment on chromatin, in conjunction with an increase in PRC2/G9A/GLP/KDM7A activities promoted a decline in Rest expression. These findings suggest a lineage-context specific paradoxical role for KDM7A in the regulation of Rest expression in CGNPs. In human SHH-MBs (SHH-α and SHH-β) where elevated REST gene expression is associated with poor prognosis, up- or downregulation of KDM7A caused a significant worsening in patient survival. Our studies are the first to implicate KDM7A in REST regulation and in MB biology.
Distinct Epithelial Cell Profiles in Normal Versus Induced-Congenital Diaphragmatic Hernia Fetal Lungs
Authors: Gonçalves Ana N. , Correia-Pinto Jorge , Nogueira-Silva Cristina
Published: May 2022

Background

Recent studies identified a great diversity of cell types in precise number and position to create the architectural features of the lung that ventilation and respiration at birth depend on. With damaged respiratory function at birth, congenital diaphragmatic hernia (CDH) is one of the more severe causes of fetal lung hypoplasia with unspecified cellular dynamics.

Objectives

 To characterize the epithelial cell tissue in hypoplastic lungs, a careful analysis regarding pulmonary morphology and epithelial cell profile was conducted from pseudoglandular-to-saccular phases in normal versus nitrofen-induced CDH rat lungs.

Design

Our analysis comprises three experimental groups, control, nitrofen (NF) and CDH, in which the relative expression levels (western blot) by group and developmental stage were analyzed in whole lung. Spatiotemporal distribution (immunohistochemistry) was revealed by pulmonary structure during normal and hypoplastic fetal lung development. Surfactant protein-C (SP-C), calcitonin gene-related peptide (CGRP), clara cell secretory protein (CCSP), and forkhead box J1 (FOXJ1) were the used molecular markers for alveolar epithelial cell type 2 (AEC2), pulmonary neuroendocrine, clara, and ciliated cell profiles, respectively.

Results

Generally, we identified an aberrant expression of SP-C, CGRP, CCSP, and FOXJ1 in nitrofen-exposed lungs. For instance, the overexpression of FOXJ1 and CGRP in primordia of bronchiole defined the pseudoglandular stage in CDH lungs, whereas the increased expression of CGRP in bronchi; FOXJ1 and CGRP in terminal bronchiole; and SP-C in BADJ classified the canalicular and saccular stages in hypoplastic lungs. We also described higher expression levels in NF than CDH or control groups for both FOXJ1 in bronchi, terminal bronchiole and BADJ at canalicular stage, and SP-C in bronchi and terminal bronchiole at canalicular and saccular stages. Finally, we report an unexpected expression of FOXJ1 in BADJ at canalicular and saccular stages, whereas the multi cilia observed in bronchi were notably absent at embryonic day 21.5 in induced-CDH lungs.

Conclusion

The recognized alterations in the epithelial cell profile contribute to a better understanding of neonatal respiratory insufficiency in induced-CDH lungs and indicate a problem in the epithelial cell differentiation in hypoplastic lungs.
Case Report: Multifocal EBV-Associated Diffuse Large B-Cell Lymphoma in a Patient With 6-MP Associated Lymphopenia With TPMT Deficiency
Authors: Müller-Scholden Lara , Deinlein Frank , Eyrich Matthias , Schlegel Paul Gerhardt , Wiegering Verena , Wölfl Matthias
Published: May 2022

Introduction

EBV associated lymphoproliferative disorders (EBV LPD) are a known complication following solid organ or hematopoietic stem cell transplantation. The disturbance of the immune system leads to a lack of control of latent EBV-infected B-cells, as control by T-cells is mandatory to prevent uninhibited cell proliferation. EBV LPD in other settings is less frequent and etiology and pathogenesis are not completely understood.

Case Presentation

We present the case of an 18-year old adolescent suffering from lymphoblastic T-cell lymphoma who developed a life-threatening EBV associated B-cell lymphoma while he was under therapy with 6-MP (6- mercaptopurine). An underlying homozygous TPMT (thiopurine S-methyltransferase) deficiency with subsequent insufficient degradation of 6-MP was identified as contributory for the development of a distinct lymphopenia leading to EBV LPD. The patient was successfully treated by discontinuation of 6-MP and initiating rituximab monotherapy.

Discussion

Rare cases of EBV LPD during therapy with 6-MP are reported in patients with leukemia, but no data about TPMT pharmacogenomics are available. In contrast the disease development in the presented case may be explained by the iatrogenic immunosuppression in the context of TPMT deficiency. While using 6-MP testing of genetic variations is not required for every protocol, although the use of thiopurines in patients with TPMT deficiency can cause severe immunosuppression. Our case suggests that insufficient degradation of 6-MP can have significant consequences despite dose reduction.

Conclusion

When using thiopurines, TPMT genetics should be initiated and strict drug monitoring and dose adjusting must be performed by a specialized center.
Biotechnological Combination for Co-contaminated Soil Remediation: Focus on Tripartite “Meta-Enzymatic” Activity
Authors: Tartaglia Maria , Zuzolo Daniela , Postiglione Alessia , Prigioniero Antonello , Scarano Pierpaolo , Sciarrillo Rosaria , Guarino Carmine
Published: May 2022

Soil pollution is a pressing problem requiring solutions that can be applied without large-scale side effects directly in the field. Phytoremediation is an effective strategy combining plant and root-associated microbiome to immobilize, degrade, and adsorb pollutants from the soil. To improve phytoremediation, it is necessary to think of plants, fungi, and bacteria not as individual entities, but as a meta-organism that reacts organically, synergistically, and cooperatively to environmental stimuli. Analyzing the tripartite enzymatic activity in the rhizosphere is necessary to understand the mechanisms underlying plant–microorganism communication under abiotic stress (such as soil pollution). In this work, the potential of a microbial consortium along with a plant already known for its phytoremediation capabilities, Schedonorus arundinaceus (Scheb.) Dumort., was validated in a mesocosm experiment with pluricontaminated soil (heavy metals, PAHs, and PCBs). Chemical analyses of the soil at the beginning and end of the experiment confirmed the reduction of the main pollutants. The microscopic observation and chemical analyses confirmed the greater root colonization and pollutant removal following the microbial treatment. To obtain a taxonomic and functional picture, tripartite (plant, fungi, and bacteria) enzyme activity was assessed using a metatranscriptomic approach. Total RNA was extracted from a sample of rhizosphere sampled considering 2 centimeters of root and soil attached. From the total reads obtained, mRNAs were filtered, and analysis focused on reads identified as proteins with enzymatic activity. The differential analysis of transcripts identified as enzymes showed that a general increase in potential enzyme activity was observed in the rhizosphere after our biotechnological treatment. Also from a taxonomic perspective, an increase in the activity of some Phyla, such as Actinobacteria and Basidiomycota, was found in the treated sample compared to the control. An increased abundance of enzymes involved in rhizospheric activities and pollutant removal (such as dehydrogenase, urease, and laccase) was found in the treated sample compared to the control at the end of the experiment. Several enzymes expressed by the plant confirmed the increase in metabolic activity and architectural rearrangement of the root following the enhancement of the rhizospheric biome. The study provides new outcomes useful in rhizosphere engineering advancement.
The Role of Case Syncretism in Agreement Attraction: A Comprehension Study
Authors: Slioussar Natalia , Magomedova Varvara , Makarova Polina
Published: May 2022

Many production and comprehension experiments have studied attraction errors in agreement, primarily in number (e.g., “The key to the cabinets were rusty”). Studies on gender agreement attraction are still sparse, especially in comprehension. We present two self-paced reading experiments on Russian focusing on the role of syncretism in this phenomenon. Russian nouns are inflected for case and number, and some forms have the same inflections (are syncretic). In several experiments on Slovak, it was shown that both head and attractor syncretism play a role for gender agreement in production. We demonstrate for the first time that this is also the case in comprehension. The role of head noun syncretism has not been analyzed in any previous comprehension studies, also for number agreement. We conclude that syncretic forms create uncertainty, which is crucial for agreement disruption. These results are better compatible with retrieval approaches to agreement attraction. We discuss the implications of our findings for the nature of the retrieval cues used to establish morphosyntactic dependencies. The question whether case marking modulates agreement attraction in comprehension has also been addressed in a study on Armenian, and it found no evidence of such influence. We offer an explanation of the conflicting findings from several studies based on the syntactic constructions they used as materials.
Association of the Onset of Self-Feeding With Subsequent Suspected Developmental Coordination Disorder: A Prospective Cohort Study in China
Authors: Hua Jing , Williams Gareth J. , Barnett Anna L. , Zhang Jiajia , Jin Hua , Xu Manyun , Chen Juan , Zhou Yingchun , Gu Guixiong , Du Wenchong
Published: May 2022

Background

Successful self-feeding reflects the readiness of early motor development and environmental impacts, and the onset of self-feeding as a developmental milestone might be a predictor of subsequent motor development in children. In this study, we explored the association between the onset of self-feeding and childhood risk of Developmental Coordination Disorder in children from one-child and two-child families.

Methods

We conducted a data-linkage prospective cohort study from 38 kindergartens in 6 cities in China. A total of 11,727 preschoolers aged 3–6 years old were included in the final analysis and were assessed with the Movement Assessment Battery for Children-second edition (MABC-2) Test. The information on early self-feeding onset was obtained from parents. The mixed and multi-level logistic models utilizing a random intercept were used to investigate the associations between the onset time of self-feeding and subsequent motor performance.

Results

The results showed that, compared with those beginning self-feeding at or younger than 12 months of age, children starting self-feeding at 13–24, 25–36, and later than 36 months, showed a decrease in their total MABC-2 scores of 2.181, 3.026, and 3.874, respectively; and had an increased risk of suspected DCD by 36.0, 101.6, 102.6%, respectively; they also had 30.2, 46.6, 71.2% increased prevalence of at risk of suspected DCD, when adjusting for both child and family characteristics (each p < 0.05). Significant associations were observed in fine motor, gross motor, and balance subtests (each p < 0.05) in groups with a delayed onset of self-feeding. However, the strength of the associations was mitigated in the fine motor and balance subtests in children with a sibling.

Conclusion

The delayed onset time of self-feeding acts as an early behavioral marker for later childhood motor impairment. Moreover, children with a sibling may benefit from additional interaction and their motor developmental pattern may be affected by the presence of a sibling.
The Molecular Basis of Resilience: A Narrative Review
Authors: Ryan Megan , Ryznar Rebecca
Published: May 2022

Resilience refers to the adaptability of a person – an ability to “bounce-back” from stressors. We question if resilience can be strengthened, potentially to decrease the risk of stress-related disorders. Unfortunately, the molecular origins of resilience are complicated and not yet well understood. In this review, we examine the various physiological biomarkers of resilience, including the associated genes, epigenetic changes, and protein biomarkers associated with resilient phenotypes. In addition to assessing biomarkers that may indicate higher levels of resilience, we also review at length the many biomarkers that confer lower levels of resilience and may lead to disorders of low resilience, such as anxiety and depression. This large and encompassing review may help to identify the possible therapeutic targets of resilience. Hopefully these studies will lead to a future where stress-related disorders can be prevented, rather than treated.
Basal Cisternostomy for Severe TBI: Surgical Technique and Cadaveric Dissection
Authors: Giammattei L. , Starnoni D. , Messerer M. , Daniel R. T.
Published: May 2022

Introduction

Cisternostomy is emerging as a novel surgical technique in the setting of severe brain trauma. Different surgical techniques have been proposed with a variable degree of epidural bone work. We present here the surgical technique as it is currently performed in our Institution.

Methods

Anatomical dissection of one adult cadaveric head, injected and non-formalin fixed was perfomed. A large right fronto-temporo-parietal craniotomy was accomplished. Extradural sphenoidal drilling till opening of the superior orbital fissure was performed. The microsurgical anatomy of basal cisternostomy was then explored.

Results

A step by step description of the surgical technique, enriched with cadaveric and intraoperative images, was made.

Conclusion

Basal cisternostomy is a promising surgical technique that does not necessarily include complex surgical maneuvers. Trained neurosurgeon can safely implement it in their clinical practice.
Specific Heat Capacity and Thermal Conductivity Measurements of PLA-Based 3D-Printed Parts with Milled Carbon Fiber Reinforcement
Authors: Blanco Ignazio , Cicala Gianluca , Recca Giuseppe , Tosto Claudio
Published: May 2022

This research focuses on the thermal characterization of 3D-printed parts obtained via fused filament fabrication (FFF) technology, which uses a poly(lactic acid) (PLA)-based filament filled with milled carbon fibers (MCF) from pyrolysis at different percentages by weight (10, 20, 30 wt%). Differential scanning calorimetry (DSC) and thermal conductivity measurements were used to evaluate the thermal characteristics, morphological features, and heat transport behavior of the printed specimens. The experimental results showed that the addition of MCF to the PLA matrix improved the conductive properties. Scanning electron microscopy (SEM) micrographs were used to obtain further information about the porosity of the systems.
Machine Learning Short-Term Energy Consumption Forecasting for Microgrids in a Manufacturing Plant
Authors: Slowik Maciej , Urban Wieslaw
Published: May 2022

Energy production and supply are important challenges for civilisation. Renewable energy sources present an increased share of the energy supply. Under these circumstances, small-scale grids operating in small areas as fully functioning energy systems are becoming an interesting solution. One crucial element to the success of micro-grid structures is the accurate forecasting of energy consumption by large customers, such as factories. This study aimed to develop a universal forecasting tool for energy consumption by end-use consumers. The tool estimates energy use based on real energy-consumption data obtained from a factory or a production machine. This model allows the end-users to be equipped with an energy demand prediction, enabling them to participate more effectively in the smart grid energy market. A single, long short-term memory (LSTM)-layer-based artificial neural network model for short-term energy demand prediction was developed. The model was based on a manufacturing plant’s energy consumption data. The model is characterised by high prediction capability, and it predicted energy consumption, with a mean absolute error value of 0.0464. The developed model was compared with two other methodologies.
Valve Regulated Lead Acid Battery Evaluation under Peak Shaving and Frequency Regulation Duty Cycles
Authors: Shamim Nimat , Viswanathan Vilayanur V. , Thomsen Edwin C. , Li Guosheng , Reed David M. , Sprenkle Vincent L.
Published: May 2022

This work highlights the performance metrics and the fundamental degradation mechanisms of lead-acid battery technology and maps these mechanisms to generic duty cycles for peak shaving and frequency regulation grid services. Four valve regulated lead acid batteries have been tested for two peak shaving cycles at different discharge rates and two frequency regulation duty cycles at different SOC ranges. Reference performance and pulse resistance tests are done periodically to evaluate battery degradation over time. The results of the studies are expected to provide a valuable understanding of lead acid battery technology suitability for grid energy storage applications.
Study on the Environmental Efficiency of the Chinese Cement Industry Based on the Undesirable Output DEA Model
Authors: Tu Hongxing , Dai Wei , Xiao Xu
Published: May 2022

In recent decades, China’s cement production has been the highest in the world, but the extensive development model, which has been formed for a long time, has brought serious damage to the natural environment. In order to promote the transformation of the production mode of China’s cement industry, this paper adopts the nonparametric frontier method to analyze the environmental efficiency of China’s cement manufacturing industry using the input–output and pollutant emission data of China’s cement manufacturing industry from 2004 to 2016. The results show that the overall environmental efficiency of China’s cement industry is low, and there is still much room for improvement. Moreover, there are serious imbalances from very low to very high between different regions. Further investigation found that during the study period, strict environmental supervision brought an average compliance cost of CNY 23.41 billion to China’s cement manufacturing industry, but the overall environmental efficiency increased by 23.9 percentage points. Based on these findings, we believe that the focus of environmental supervision of China’s cement manufacturing industry at this stage is to reduce pollution emissions, and force cement enterprises to carry out technological innovation through mandatory emission reduction measures. When formulating policies, the Chinese government needs to explore the best way for environmental supervision between minimizing compliance costs and maximizing efficiency, so as to promote the sustainable development of China’s cement manufacturing industry.
Advanced Control Techniques for Wind/Solar/Battery Systems
Authors: Vukadinović Dinko
Published: May 2022

This Editorial summarizes the papers of the Special Issue entitled ‘Advanced Control Techniques for Wind/Solar/Battery Systems’ published in Energies. The Special Issue includes four scientific articles published in 2021 and 2022 in the field of quasi-Z-Source inverter control, photovoltaic energy conversion, battery charge control, wind turbine speed control, and solar irradiance prediction. New scientific achievements with experimental verifications of the achieved results are presented in all articles.
In Situ Growth of COF on PAN Nanofibers to Improve Proton Conductivity and Dimensional Stability in Proton Exchange Membranes
Authors: Meng Xiaoyu , Lv Yinan , Wen Jihong , Li Xiaojing , Peng Luman , Cong Chuanbo , Ye Haimu , Zhou Qiong
Published: May 2022

Perfluorosulfonic acid (PFSA) polymer is considered as a proton exchange membrane material with great potential. Nevertheless, excessive water absorption caused by abundant sulfonic acid groups makes PFSA have low dimensional stabilities. In order to improve the dimensional stability of PFSA membranes, nanofibers are introduced into PFSA membranes. However, because nanofibers lack proton conducting groups, it usually reduces the proton conductivities of PFSA membranes. It is a challenge to improve dimensional stabilities while maintaining high proton conductivities. Due to the structural designability, covalent organic frameworks (COFs) with proton conductive groups are chosen to improve the overall performance of PFSA membranes. Herein, COFs synthesized in situ on three-dimensional PAN nanofibers were introduced into PFSA to prepare PFSA@PAN/TpPa-SO3H sandwiched membranes. The PFSA@PAN/TpPa-SO3H-5 composite membrane exhibited outstanding proton conductivity, which reached 260.81 mS·cm−1 at 80 °C and 100% RH, and only decreased by 4.7% in 264 h. The power density of a single fuel cell with PFSA@PAN/TpPa-SO3H-5 was as high as 392.7 mW·cm−2. Compared with the pristine PFSA membrane, the conductivity of PFSA@PAN/TpPa-SO3H-5 increased by 70.0 mS·cm−1, and the area swelling ratio decreased by 8.1%. Our work provides a novel strategy to prepare continuous proton transport channels to simultaneously improve conductivities and dimensional stabilities of proton exchange membranes.
Missing Data Imputation in the Internet of Things Sensor Networks
Authors: Agbo Benjamin , Al-Aqrabi Hussain , Hill Richard , Alsboui Tariq
Published: May 2022

The Internet of Things (IoT) has had a tremendous impact on the evolution and adoption of information and communication technology. In the modern world, data are generated by individuals and collected automatically by physical objects that are fitted with electronics, sensors, and network connectivity. IoT sensor networks have become integral aspects of environmental monitoring systems. However, data collected from IoT sensor devices are usually incomplete due to various reasons such as sensor failures, drifts, network faults and various other operational issues. The presence of incomplete or missing values can substantially affect the calibration of on-field environmental sensors. The aim of this study is to identify efficient missing data imputation techniques that will ensure accurate calibration of sensors. To achieve this, we propose an efficient and robust imputation technique based on k-means clustering that is capable of selecting the best imputation technique for missing data imputation. We then evaluate the accuracy of our proposed technique against other techniques and test their effect on various calibration processes for data collected from on-field low-cost environmental sensors in urban air pollution monitoring stations. To test the efficiency of the imputation techniques, we simulated missing data rates at 10–40% and also considered missing values occurring over consecutive periods of time (1 day, 1 week and 1 month). Overall, our proposed BFMVI model recorded the best imputation accuracy (0.011758 RMSE for 10% missing data and 0.169418 RMSE at 40% missing data) compared to the other techniques (kNearest-Neighbour (kNN), Regression Imputation (RI), Expectation Maximization (EM) and MissForest techniques) when evaluated using different performance indicators. Moreover, the results show a trade-off between imputation accuracy and computational complexity with benchmark techniques showing a low computational complexity at the expense of accuracy when compared with our proposed technique.
Achieving Minimum Standards for Infection Prevention and Control in Sierra Leone: Urgent Need for a Quantum Leap in Progress in the COVID-19 Era!
Authors: Fofanah Bobson Derrick , Abrahamyan Arpine , Maruta Anna , Kallon Christiana , Thekkur Pruthu , Kamara Ibrahim Franklyn , Njuguna Charles Kuria , Squire James Sylvester , Kanu Joseph Sam , Bah Abdulai Jawo , Lakoh Sulaiman , Kamara Dauda , Hermans Veerle , Zachariah Rony
Published: May 2022

Introduction: Good Infection prevention and control (IPC) is vital for tackling antimicrobial resistance and limiting health care-associated infections. We compared IPC performance before (2019) and during the COVID-19 (2021) era at the national IPC unit and all regional (4) and district hospitals (8) in Sierra Leone. Methods: Cross-sectional assessments using standardized World Health Organizations IPC checklists. IPC performance scores were graded as inadequate = 0–25%, basic = 25.1–50%, intermediate = 50.1–75%, and advanced = 75.1–100%. Results: Overall performance improved from ‘basic’ to ‘intermediate’ at the national IPC unit (41% in 2019 to 58% in 2021) and at regional hospitals (37% in 2019 to 54% in 2021) but remained ‘basic’ at district hospitals (37% in 2019 to 50% in 2021). Priority gaps at the national IPC unit included lack of: a dedicated IPC budget, monitoring the effectiveness of IPC trainings and health care-associated infection surveillance. Gaps at hospitals included no assessment of hospital staffing needs, inadequate infrastructure for IPC and lack of a well-defined monitoring plan with clear goals, targets and activities. Conclusion: Although there is encouraging progress in IPC performance, it is slower than desired in light of the COVID-19 pandemic. There is urgent need to mobilize political will, leadership and resources and make a quantum leap forward.