bluuebunny/crossref_metadata_2025 huggingface dataset:
Proteomic profiling of serum exosomes reveals acute phase response and promotion of inflammatory and platelet activation pathways in patients with heat stroke
Authors: Li Yue , Li Huan , Maegelev Marc , Su Lei , Gu Zhengtao
Published: May 2022

Background: The pathological mechanism of heat stroke (HS) involves the acute phase response, unbalanced immunological/inflammatory reactions, and coagulation initiation, especially platelet activation. Although exosomes contain proteins involved in these biological processes, their protein cargo levels and potential roles in HS remain unknown. This study explored the serum exosome protein expression patterns after HS and their potential roles in the pathogenesis of HS.Methods: Ten patients diagnosed with HS were chosen for blood sample collection upon admission to the intensive care unit (six with severe HS and four with mild HS). Samples from six healthy volunteers were included as control. Using ultracentrifugation, exosomes were prudently isolated, and their protein contents were profiled using liquid chromatography–tandem mass spectrometry analysis with isobaric tags for relative and absolute quantification-based proteomics.Results: Compared with healthy volunteers, patients with HS showed significant changes in the levels of 33 exosomal proteins (23 upregulated and 10 downregulated). The most upregulated proteins included serum amyloid A-1 (SAA-1), von Willebrand factor (vWF), S100A8, and histone H3, among others. In addition, SAA-1, vWF, PAF, S100A8, and histone H3 were more enriched in the exosomes from patients with severe HS than observed in those with mild HS. Gene ontology analysis revealed that the HS-modulated exosomal proteins were mostly related to inflammatory response, including acute-phase response, platelet activation/degranulation and innate immune response. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed significant enrichment of proteins in the IL-17 signaling pathway, platelet activation, neutrophil extracellular trap formation, Fc epsilon RI signaling pathway, chemokine signaling pathway, and NOD-like receptor signaling pathway, among others. Several serum exosomal proteins, including SAA-1, vWF, and S100A8, which are related to the acute phase, inflammatory response, and platelet activation, were confirmed to be elevated in patients with HS, and were significantly correlated with disease severity, organ dysfunction, and death.Conclusion: Overall, this study confirms the potential role of the serum exosomal proteome in the inflammatory response and platelet activation in HS, suggests the pathological mechanisms underlying HS-induced injuries, and recommends reliable exosomal biomarkers for predicting HS prognosis.Trial registration: Not applicable.
Missing data analysis and prediction: A Google cluster case study
Authors: Wang Hui , Jiang Chunmao , Xie Bin
Published: May 2022

As the number of running tasks increases dramatically on a cloud platform , the probability of task failure increases. Among the many reasons affecting task failure, the lack of information is straightforward and fatal. Suppose we can predict whether a task will generate missing information and take measures in advance. In that case, we can effectively reduce the probability of task failure and also avoid ineffective use of resources. This paper investigates the missing information of tasks and proposes a missing prediction algorithm based on an improved Mixture of Gaussian (GMM) algorithm. Specifically, we deeply analyze and process the cloud task information based on the latest cloud platform data released by Google Inc in 2019. Several clustering algorithms are used to make experimental comparisons to determine their suitability for prediction. Finally, we developed an optimized GMM algorithm to build the prediction model. The experiments show the model is fast and accurate and can significantly save system time and resource utilization.
The COVID-19 and the air quality in Tunisia during 2020 and 2021 years
Authors: Abderrazak Arif
Published: May 2022

The first case of COVID-19 was reported in Wuhan, China at December 2019. A month later, that epidemic turned into a national crisis, with infected individuals diagnosed all over China. In early March 2020, the World Health Organization (WHO) declared that the Wuhan epidemic has turned into a global pandemic. Many European countries have started to know several cases affected by this coronavirus, which is known to be highly contagious. The WHO has launched several recommendations to curb the spread of this virus and to call the general confinement establishment in the affected countries. Tunisia quickly took this step on March 22, 2020 and announced immediately general confinement for two weeks, renewable according to the tests results. Factories have been closed to limit human damage. International flights have been halted and the majority of government and private services have been halted except minimum and emergency services. Following these successive events, the air quality has improved markedly during the confinement period. NASA scientists say the reduction in NO2 pollution first appeared near Wuhan, Northern Italy and France experienced a reduction of nearly 50% of their NO2 emissions during this first confinement period (March-April, 2020) and been reduced by almost 30% in China. In Tunisia, Nitrogen dioxide (NO2), Sulfur dioxide (SO2) and Carbon monoxide (CO) showed a remarkable decrease in the North and the Center of Tunisia of more than 40% during this period mainly linked to the reduction in emission from road traffic and industries. Also these pollutant gases concentrations have knew a nearly 50% reduction during the 3rd pandemic wave during the period of January-April 2021. Consequently, the air quality has improved significantly in Tunisia and around the world.
New Minkowski and related inequalities via general kernels and measure
Authors: Iqbal Sajid , Samraiz Muhammad , Khan Muhammad Adil , Rahman Gauhar , Nonlaopon Kamsing
Published: May 2022

In this article, we introduce a class of functions U(p) with integral representation defined over measure space with σ-finite measure. The main purpose of this paper is to extend the Minkowski and related inequalities by considering general kernels. As a consequence of our general results, we connect our results with various variants for the fractional integrals operators. Such applications have wide use and importance in the field of applied sciences.AMS Subject Classification: 26D15; 26D10; 26A33; 34B27
Development of an integrated biomarker for prediabetes and T2DM in Chinese individuals through serum lipidomic analyses
Authors: Yang Jiaying , Yan Han , Wang Zhigang , Yan Dan , Guo Na
Published: May 2022

By fully elucidating the pathophysiological basis for prediabetes and T2DM in affected individuals, it may be possible to intervene at an earlier time point such that disease progression can be arrested. Dyslipidemia is a hallmark of T2DM, and as such, analyses of lipid metabolic profiles in affected patients have the potential to permit the development of an integrated lipid metabolite-based biomarker model that can facilitate early patient diagnosis and treatment. Herein, untargeted and targeted lipidomics approaches were used to analyze serum samples from newly diagnosed 93 Chinese participants in discovery cohort and 440 in validation cohort via UHPLC-MS and UHPLC-MS/MS. Establish a new biomarker model through appropriate ROC analyses. The integrated biomarker model developed herein exhibited superior diagnostic performance when used to detect prediabetes and T2DM patients within this cohort. The results of western blot analysis of frozen adipose tissue from 3- and 12-week GK rats also confirmed that acid sphingomyelinase is responsible for significant disruptions in ceramide and sphingomyelin homeostasis in prediabetes and T2DM patients. Network analyses of the biomarkers associated with this biosignature suggested that the most profoundly affected lipid metabolism pathways in the context of diabetes include de novo ceramide synthesis, sphingomyelin metabolism, and additional pathways associated with phosphatidylcholine synthesis. Together, these results offer new biological insights regarding the role of serum lipids in the context of insidious T2DM development, and may offer new avenues for future diagnostic and/or therapeutic research.
Simulation of the E-Defense 2015 test on a 10-storey building using macro-models
Authors: Janevski Antonio , Kang Jae-Do , Isaković Tatjana
Published: May 2022

The capabilities of certain standard macro numerical models were tested by simulating the shaking table experiment on a full-scale 10-storey fixed-base building at the largest shaking table in the E-Defense centre in Japan. The lumped plasticity model for columns and beams, the force-displacement version of the MVLEM (multiple-vertical-line-element model) for walls and the scissors model with rigid links for beam-column joints were evaluated. A reasonably good simulation of the experiment was achieved using the models' standard input parameters in most cases. However, a few input parameters were modified based on the experimental data, particularly when modelling joints, which were relatively weak; therefore, they were considerably damaged during the strongest seismic excitations. The essential parameters that influenced the response were identified and analysed. The most important parameters were the effective width of the slabs, the ratio of the strength, stiffness and amount of dissipated energy in the joints as compared to the beams and columns, and the reduced initial stiffness of the structure.
The techniques used to treat complex posterior urethral strictures in boys and the long-term follow up outcomes
Authors: Zhu Weidong , Song Lujie , Sa Yinglong , Xu Yuemin , Fu Qiang
Published: May 2022

Purpose
To explored the curative effects of various surgical methods used to treat complicated posterior urethral strictures in boys and the long-term complication.
Methods
We retrospectively studied 28 boys under 14 years of age with complicated posterior urethral strictures treated at our hospital from January 2015 to December 2020. Urethral angiography revealed posterior urethral strictures. Twelve had previously failed urethral surgery; four had urethral fistulae. All underwent end-to-end urethral anastomoses via a transperineal, inferior pubic approach. We freed the distal end of the urethra, split the penile cavernous septum, partially resected the lower edge of the pubic symphysis, and rerouted the urethra under a corpus cavernosum to reduce the tension of the urethral anastomosis.
Results
All boys were 2–14 years of age at the time of surgery (mean 6.3 years). The urethral strictures were 3–5.5 cm in length (mean 4.2 cm). Catheters were removed 4 weeks postoperatively. The postoperative follow-up time was 4–72 months (mean 36.8 months). Twenty-four patients exhibited unobstructed urination after a single operation. The maximum urinary flow rate was 15–22 mL/s (average 17.8 mL/s); the success rate was 85.7%. Two patients required second urethral end-to-end anastomoses; urination became normal postoperatively. Two continued to exhibit cystostomies, and two evidenced mild incontinence. Of the six children who have attained puberty, two report erectile dysfunction.
Conclusion
End-to-end urethral anastomosis via a transperineal inferior pubic approach is an ideal treatment for posterior urethral strictures in boys. The complications include incontinence and erectile dysfunction, and require long-term follow-up.
Mechanosensitive Traction Force Generation is Regulated by the Neutrophil Activation State
Authors: Witt Hadley , Yan Zicheng , Henann David , Franck Christian , Reichner Jonathan
Published: May 2022

The generation of traction forces by neutrophils regulates many crucial effector functions responsible for host defense, such as attachment, spreading, migration, phagocytosis, and NETosis. The activation state of the cell is a strong determinant of the functional efficacy of the neutrophil; however, the effect of activation on traction force production has not yet been determined experimentally. Previously, the mapping of cellular-generated forces produced by human neutrophils via a Traction Force Microscopy (TFM) method has required a three-dimensional imaging modality to capture out-of-plane forces, such as confocal or multiphoton techniques. A method newly developed in our laboratories can capture out-of-plane forces using only a two-dimensional imaging modality. This novel technique—combined with a topology-based single particle tracking algorithm and finite element method calculations—can construct high spatial frequency three-dimensional traction fields, allowing for traction forces in-plane and out-of-plane to the substrate to now be differentially visualized and quantified with a standard epifluorescence microscope. Here we apply this technology to determine the effect of neutrophil activation on force generation. Sepsis is a systemic inflammatory response that causes dysregulated neutrophil activation in vivo. We found that neutrophils from septic patients produced greater total forces than neutrophils from healthy donors and that the majority of this dysregulation occurred in-plane to the substrate. Ex vivo activation of neutrophils from healthy donors showed differential consequences depending on activation stimuli with mechanosensitive force decreases observed in some cases. These findings demonstrate the feasibility of epifluorescence-based microscopy in mapping traction forces to ask biologically significant questions regarding neutrophil function.
Impaired expression of the TWIK2 K+ efflux channel leads to reduced IL-1 family cytokine production in patients with nontuberculous mycobacterial lung disease
Authors: Jung Bock-Gie , Dean Kristin , Wadle Carly , Samten Buka , Tripathi Deepak , Wallace Richard J. , Brown-Elliott Barbara A. , Tucker Torry , Idell Steven , Philley Julie V. , Vankayalapati Ramakrishna
Published: May 2022

Background: Nontuberculous mycobacteria (NTM) causes disseminated disease in patients with immunodeficiency and pulmonary disease in individuals without obvious immunodeficiency. There is no clear information on how NTM pulmonary disease (NTMPD) develops in immunocompetent hosts. This profile study was initiated to gain insight into the immunological factors that predispose persons to pulmonary NTM infections.Methods: Blood samples were obtained from 15 pairs of NTMPD patients and age-matched healthy household contacts. Peripheral blood mononuclear cells (PBMCs) were stimulated with the heat-killed M. avium complex (MAC). A total of 34 cytokines and chemokines were evaluated in PBMCs culture supernatants and plasma using multiplex immunoassays. The mRNA expression of TLR2, P2X7R, TWIK2, THIK2 and TREK1 was measured by quantitative real-time PCR. In mechanistic studies, blood samples were obtained from healthy volunteers who had not been diagnosed or treated for NTM.Results: Interleukin (IL)-1β, IL-18, IL-1α and IL-10 production were significantly reduced in response to MAC in PBMCs of NTMPD patients compared with PBMCs of their healthy household contacts. RANTES was the only chemokine significantly elevated in the plasma of NTMPD patients compared with plasma of their healthy household contacts. TLR2 and TWIK2 expression were impaired in response to MAC in PBMCs of NTMPD patients compared with PBMCs of their healthy household contacts. RANTES had no effect on IL-1β production by macrophages infected with MAC. A TLR2 inhibitor decreased IL-1β, IL-18, IL-1α and IL-10 production by MAC-stimulated PBMCs and monocytes. A TWIK2 inhibitor decreased the production of IL-1β, IL-18, and IL-1α, but not IL-10, by MAC-stimulated PBMCs and monocytes.Conclusions: These findings suggest that impaired TWIK2 results in decreased production of IL-1 family cytokines (IL-1β, IL-18, and IL-1α) in response to MAC, consequentially may increase susceptibility to NTM pulmonary infection.
Pharmacodynamic modeling of moderate sedation and rationale for dosing using midazolam, propofol and alfentanil
Authors: Liou Jing-Yang , Kuo I-Ting , Chang Weng-Kuei , Ting Chien-Kun , Tsou Mei-Yung
Published: May 2022

Purpose:Regulations have broadened to allow moderate sedation administration for gastrointestinal endoscopy by non-anesthesia personnel. The line between moderate and deep sedation is ambiguous. Deep sedation offers patient comfort as well as greater safety concerns. Unintended deep sedation can occur if drug interactions are overlooked. We present a pharmacodynamic model for moderate sedation using midazolam, alfentanil and propofol. The model is suitable for training and devising rationales for appropriate dosing.Methods:The study consists of two parts: modeling and validation. In modeling, patients scheduled for esophagogastroduodenoscopy (EGD) or colonoscopy sedation are enrolled. The modified observer’s assessment of alertness/sedation (MOAA/S) score < 4 is defined as loss of response to represent moderate sedation. Two patient groups receiving bronchoscopy or endoscopic retrograde cholangiopancreatography (ERCP) are used for validation. Model performance is assessed by receiver operating characteristic (ROC) curves and area under the curve (AUC). Simulations are performed to demonstrate how the model is used to rationally determine drug regimen for moderate sedation.Results:Interaction between propofol and alfentanil is stronger than the other pairwise combinations. Additional synergy is observed with three drugs. ROC AUC is 0.83 for the modeling group, and 0.96 and 0.93 for ERCP and bronchoscopy groups respectively. Model simulation suggests that 1mg midazolam, 250μg alfentanil and propofol maximally benefits from drug interactions and suitable for moderate sedation.Conclusion:We demonstrate the accurate prediction of a three-drug response surface model for moderate sedation and simulation suggests a rational dosing strategy for moderate sedation with midazolam, alfentanil and propofol.
Impact of psychological stress during the COVID 19 pandemic on the quality of life of health care workers in mental health hospitals in Egypt
Authors: nagar zeinab mohamed el , Hammed Marwa Abdel Meguid , Mohamed Mohamed Youssef , Massoud Yasmine Mahmoud , Aufa Ola Mohamad
Published: May 2022

AbstractBackground: COVID-19 outbreak resulted in negative mental health consequences such as depression, anxiety, and stress, particularly among healthcare workers. Objective: To evaluate health care workers' mental well-being and explore the reasons standing beyond their concerns and fears. Methods: From January to June 2021, we recruited 107 health care workers from psychiatric hospitals. The translated Arabic version COVID stress scale was used to assess their level of stress. The WHO Quality of Life - BREF score was used to assess their level of affection for their quality of life. Results: The majority of our sample (45.8%) was between the ages of 20 and 30. Females made up 37.8% of the group. The majority of them were infected during the first wave. The COVID Stress Scale (CSS) revealed that 21.5% of participants had no signs or symptoms of depression, while 55.1% had mild symptoms. Only 23.4% of our sample had moderate to severe symptoms. When categorized by domains, the mean CSS score was 12.21 ± 5.62 for the danger subscale, 6.18 ± 4.07 for the socioeconomic subscale, 8.44 ± 4.58 for the xenophobia subscale, 7.99 ± 4.18 for the contamination subscale, 5.87 ± 4.32 for traumatic subscale, and 11.13 ± 6.15 for compulsive subscale. The mean scores for the WHO QOL-brief score for the physical aspect were 91.51 ± 21.5, 75.25 ± 12.84 for the psychological aspect, 40.42 ± 8.52 for the social aspect, and 96.26 ± 17.76 for the environmental aspect. Conclusion: we discovered that healthcare workers experienced high levels of stress, particularly during and after COVID -19 pandemic waves.
A Comparative Study for Various Morphological Characters between Diploid and Triploid Watermelon
Authors: PADHAN ABHILASH , Kumar T. Pradeep
Published: May 2022

Breeding activities to generate seedless cultivars of fruits and vegetables have been initiated around the world due to its increased market demand and consumer acceptance. Seedless fruits and vegetables are becoming increasingly popular across the world due to their higher flesh content, enhanced sweetness and ease of consumption thanks to the lack of seeds. Polyploidy breeding is a very important aspect in horticultural crops to create genetic and morphological variation among horticultural crops. Seedless watermelons are produced through polyploidy breeding by doubling the chromosome number and crossing between normal diploid(2x) with colchicine induced tetraploids (4x).The present study was carried out in the year (May-July)2015 at the High-tech seed testing laboratory under the Department of Vegetable Science, Kerala Agriculture University, Trissur, India to differentiate between the normal diploid(2x) watermelon (var. Sugar Baby) with the yellow seedless triploid (3x) watermelon hybrid var. Swarna (KAU-CL-TETRA-1 × CL-5). The true leaf samples collected from 10 numbers of diploid (var. Sugar Baby) and 7 numbers of triploid (var. Swarna) seedlings of watermelon were taken for the study. In the present study chloroplast count and pollen grain study were undertaken as the morphological markers to carry out the experiment. The results obtained from the average chloroplast count of 10 samples of diploid (2x) sugar baby was 11.07 whereas the triploid (3x) Swarna hybrid was 12.11. The pollen grain of triploid (3x) was found to be not retained from red safranin stain colour being sterile in nature whereas the fertile pollen of diploid (2x) watermelon retained the stain colour.
Multi-country outbreak of monkeypox virus: phylogenomic characterization and signs of microevolution
Authors: Gomes João Paulo , Isidro Joana , Borges Vitor , Pinto Miguel , Sobral Daniel , Santos João , Mixão Verónica , Ferreira Rita , Nunes Alexandra , Santos Daniela , Duarte Silvia , Vieira Luís , Borrego Maria José , Núncio Sofia , de Oliveira Isabel , Pelerito Ana , Cordeiro Rita
Published: May 2022

The largest monkeypox virus (MPXV) outbreak described so far in non-endemic countries was identified in May 2022. Here, shotgun metagenomics allowed the rapid reconstruction and phylogenomic characterization of the first genome sequences of the MPXV outbreak variant. We observed that this MPXV belongs to the West African clade and that the ongoing outbreak most likely has a single origin. Although 2022 MPXV clustered with 2018–2019 cases epidemiologically linked to an endemic country, it segregates in a highly divergent phylogenetic branch (~ 50SNPs), likely representing a recent evolutionary jump. This hypermutation signature suggests the potential action of host APOBEC in viral evolution. We also detected ongoing microevolution (e.g., SNPs, minor variants and gene loss) during the human-to-human outbreak transmission, highlighting potential targets of adaptation. This study sheds light on MPXV adaptive evolution and indicates that genome sequencing might provide resolution to track the spread and transmission of this presumably slow-evolving dsDNA virus.
Knowledge, attitude and perception towards COVID-19 among representative educated sub-Saharan Africans: A cross-sectional study during the exponential phase of the pandemic
Authors: Udoakang* Aniefiok , Nganyewo Nora Nghochuzie , Zune Alexandra Lindsey Djomkam , Olwal Charles Ochieng’ , Etim Nsikak-Abasi Aniefiok , Oboh Mary Aigbiremo , Tapela Kesego , Dzabeng Francis , Adadey Samuel Mawuli , Udoh Agnes , Koné Mazo , Mutungi* Joe Kimanthi , Quashie* Peter Kojo , Awandare Gordon Akanzuwine , Paemka* Lily
Published: May 2022

Coronavirus disease 2019 (COVID-19) pandemic, caused by the Severe Acute Coronavirus 2 (SARS-CoV-2), is a global health threat with extensive misinformation and conspiracy theories. Therefore, this study investigated the knowledge, attitude and perception of sub-Saharan Africans (SSA) on COVID-19 during the exponential phase of the pandemic. In this cross-sectional survey, self-administered web-based questionnaires were distributed through several online platforms. A total of 1046 respondents from 35 SSA countries completed the survey. The median age was 33 years (18–76 years) and about half (50.5%) of them were males. More than 40% across all socio-demographic categories except participants from the Central African region (21.2%), those with vocational/secondary education (28.6%), as well as student/unemployed (35.5%), had high COVID-19 knowledge scores. Socio-demographic factors and access to information were associated with COVID-19 knowledge. Bivariate analysis revealed that independent variables, including the region of origin, age, gender, education and occupation, were significantly (p < 0.05) associated with COVID-19 knowledge. Multivariate analysis showed that residing in East (odds ratio [OR]: 7.9, 95% confidence interval (CI): 4.7–14, p < 0.001), Southern (OR: 3.7, 95% CI: 2.1–6.5, p < 0.001) and West (OR: 3.9, 95% CI: 2.9–5.2, p < 0.001) Africa was associated with high COVID-19 knowledge level. Apart from East Africa (54.7%), willingness for vaccine acceptance across the other SSA regions was < 40%. About 52%, across all socio-demographic categories, were undecided. Knowledge level, region of origin, age, gender, marital status and religion were significantly (p < 0.05) associated with COVID-19 vaccine acceptance. About 67.4% were worried about contracting SARS-CoV-2, while 65.9% indicated they would consult a health professional if exposed. More than one-third of the respondents reported that their governments had taken prompt measures to tackle the pandemic. Despite high COVID-19 knowledge in our study population, most participants were still undecided regarding vaccination, which is critical in eliminating the pandemic. Therefore, extensive, accurate, dynamic and timely education in this aspect is of ultimate priority.
A large public dataset of annotated clinical MRIs of patients with acute stroke and linked metadata
Authors: Liu Chin-Fu , Leigh Richard , Johnson Brenda , Urrutia Victor , Hsu Johnny , Xu Xin , Li Xin , Mori Susumu , Hillis Argye , Faria Andreia
Published: May 2022

To extract meaningful and reproducible models of brain function from stroke images, for both clinical and research proposes, is a daunting task severely hindered by the great variability of lesion frequency and patterns. Large datasets are therefore imperative, as well as fully automated image post-processing tools to analyze them. The development of such tools, particularly with artificial intelligence, is highly dependent on the availability of large datasets to model training and testing. We present a public dataset of 2,888 multimodal clinical MRIs of patients with acute and early subacute stroke, with manual lesion segmentation, and metadata. The dataset provides high quality, large scale, human-supervised knowledge to feed artificial intelligence models and enable further development of tools to automate several tasks that currently rely on human labor, such as lesion segmentation, labeling, calculation of disease-relevant scores, and lesion-based studies relating function to frequency lesion maps
Impact of International Expansion Strategy on the Performance of Japanese Banks
Authors: A Joseph Jr. , Harimaya Kozo
Published: May 2022

The effect of cross-border diversification on bank performance is part of the broader debate on how multinational banking and financial integration affect the global financial economy. Much of the empirical studies examining this relationship present mixed results globally. Studies on both sides of the expansion spectrum find that cross-border diversification improves bank performance but also increases bank risks leading to systemic failure. Even so, this line of debate has not been examined in the case of Japanese ‘city banks’ conducting international operations. The present study questions whether cross-border diversification improves the performance of Japanese banks and to what extent each cross-border expansion activity affects bank performance. The latter was largely ignored in previous studies. The study finds that cross-border diversification, in general, improves cost efficiency but decreases/harms profit efficiency of the banks analysed. Precisely, diversification activities such as foreign assets and foreign branch expansion present funding risks and operational inefficiency. The study concludes that, as a regional player, the current trend in cross-border expansion activities of Japanese banks requires close monitoring and supervision to prevent episodes of regional financial crises. Moreover, the current cross-border diversification strategies of these banks, especially the expansion and operation of overseas branch networks (retail banking) need to be re-examined.
A meta-analysis of PCAT1; prostate cancer-associated transcript 1 gene as a prognostic biomarker for invasive breast carcinoma
Authors: Chakrabarty Brototi , Afrin Kashifa , Siraj Md Afjalus
Published: May 2022

Prostate cancer-associated transcript 1 (PCAT1) is a long non-coding RNA (lncRNA), composed of more than 200 nucleotides. Its expression controls the proliferation, migration, invasion, and metastasis of different cancer. In the current study, we conducted a patient data derived metanalysis to study the possible role of PCAT1 as a prognostic biomarker for the invasive breast cancer (IBC). Our analysis demonstrated that, PCAT1 highly expressed in BRCA mutated BC patients’ cells compared to normal cells (fold change ~ 1.56). The overall survival rate of patients with higher PCAT1 expression significantly (p- 5.539e− 5) reduced over time. Moreover, PCAT1 was significantly altered in PR (p- 1.672e-6), HER2 (p- 3.190e-6) negative and ER (p- 3.920e-4) positive primary IBC samples. In addition, alteration of PCAT1 also correlated with the histologic grade of the IBC (p- >10− 10). On top of that, we found co-occurrence of PCAT1 with the oncogenes of CASC family i.e., CASC8, CASC11, and CASC19 in IBC. The genomic location of PCAT1, CASC8, CASC11, and CASC19 was chr8q24 and chr8q24 respectively which contains the loci responsible for different cancers including BC. In addition, the CASC8 and CASC19 overlapped with PCAT1 in genomic sequence. Taken together, PCAT1 expression is associated with poor clinical outcomes and co-occur with previously established oncogenes and biomarkers which suggest its usefulness as a prognostic biomarker for IBC.
Nuclear movement generate binuclear cells during neural transdifferentiation from human bone marrow-derived mesenchymal cells
Authors: bueno Carlos , Blanquer Miguel , Bernal David García- , Martínez Salvador , Moraleda José M
Published: May 2022

Although it has been reported that bone marrow-derived mesenchymal stromal cells (BM-MSCs) can transdifferentiate into neural cells, the findings is considered unlikely. Cytotoxic-induced cell changes and cell fusion events are alternative explanations for these observations in culture and transplantation studies, respectively.Here, we show that BM-MSCs neural transdifferentiation involves a formation of dedifferentiated cells that can then redifferentiate into neural-like cells, redifferentiate back to mesenchymal fate, or even switch lineages repeatedly without cell division. Futhermore, we have discovered that nuclei from dedifferentiated cells move within the cell adopting different morphologies and even forming binucleated cells. In addition, we noted that dedifferentiated cells position their nucleus at the forefront of the cell during cell migration.Our result show that human BM-MSCs can rapidly adopt a neural-like morphology and binuclear human BM-MSCs can be formed without being related to cell fusion events. We provide additional evidences that BM-MSCs can transdifferentiate into neural cells.
Independent Correlation Between Ischemia Modified Albumin and Parathormone in End Stage Renal Disease
Authors: Güçlü Kenan , Tur Kağan , Şahin Serdar , Güçlü Aydın
Published: May 2022

Background
Oxidative stress (OS) has an important role at development of chronic kidney disease (CKD) and progression to end stage renal disease (ESRD). Hemodialysis (HD) patients are the group which oxidative stress is found more exacerbated. Ischemia modified albumin (IMA) is a new and sensitive marker for ischemia and oxidative stress. At current study we evaluated relation between IMA and biochemical parameters in ESRD patients.
Methods and Results
Thirty-four patients with ESRD on maintenance HD were included in the study. A fasting blood sample pre-HD and post-HD blood sample at the end of the dialysis session were taken from all of the patients. Serum IMA, complete blood count and routine biochemistry parameters were measured. There was a positive correlation between alkaline phosphatase (ALP) and IMA (r = 0,268, p < 0,05), CRP and IMA (r = 0,452, p = 0,007), PTH and IMA (r = 0,436, p = 0,010), There was a negative correlation between albumin and IMA (r=-0,338, p = 0,05). Multiple regression analysis was run to predict IMA levels from PTH, CRP and creatinine the model statistically significantly predicted relation p < 0,05, R = 0,506, out of four two variables added statistically significant to the prediction, PTH (p = 0,006), CRP (p = 0,029). In multiregression analysis, IMA was found to be associated with PTH and CRP independent of creatinine value.
Conclusion
In this study, we showed for the first time that PTH is associated with IMA in ESRD patients, independent of the level of renal function. IMA may play an important role in the pathogenesis of inflammation due to increased PTH in ESRD. Further studies are needed to further elucidate this issue.
Identification and Validation of Immune-Related Gene Prognostic Signature for breast cancer
Authors: Zhou Bing , Zhang Linyi , Wang Qinyu , Pu Changqin , Guo Sixuan , Lai Shuhui , Zhang Heming , Li Wenwei , Zhou Zhibing , Chen Yuexia , Zhou Yao , Zeng Liping
Published: May 2022

Background
Although the outcome of breast cancer patients has been improved by advances in early detection, diagnosis and treatment, prognostic assessment still faces challenges due to the heterogeneity of the disease. The accumulated data indicate that there is a clear correlation between the tumor immune microenvironment and clinical outcomes.
Methods
We screened prognostic immune-relevant gene pairs (IRGPs) via univariate Cox regression analysis in the Cancer Genome Atlas (TCGA) cohort. Then, TCGA cohort were further divided into a training set (n = 755) and internal validation sets (n = 320). Least absolute shrinkage and selection operator (LASSO) Cox regression analysis was used to constituted the IRGP prognostic signature in training set. And the IRGP prognostic signature was validated in the internal TCGA validation cohort and external validation cohorts GSE20685 (n = 327), GSE42568 (n = 104), and GSE20711 (n = 88) from Gene Expression Omnibus (GEO) database. We estimated the immune cell infiltration in tumor microenvironment via CIBERSORT and ESTIMATE. We used the TIDE algorithm and stemness indices to evaluate the potential of IRGP signature as an indicator of response to immunotherapy. We used gene set enrichment analysis (GSEA) to elucidate the biological functions of the IRGP prognostic signature.
Results
We generated a IRGP prognostic signature consisting of 16 IRGPs. Subsequently, the 16 IRGPs grouped breast cancer patients into high- and low-risk groups. Survival analysis indicated that the IRGP signature possessed an independent prognostic value. The low-IRGP group exhibited a higher level of immune cell infiltration, higher expression of immune checkpoint molecules, lower tumor stemness indices, and was much more sensitive to immunotherapy. The functional enrichment analysis indicated that low IRGP value was correlated with biological processes related to immune.
Conclusion
The 16-IRGP prognostic signature was developed to provide new insights for the identification of high-risk breast cancer and the evaluation of the possibility of immunotherapy in personalized breast cancer treatment.
Innovation and Practice of Music Education Path in Colleges and Universities Under the Popularization of 5g Network
Authors: Song Xi
Published: May 2022

The value of music education in society and culture cannot be overstated. For students with exceptional needs, music education plays a vital role. Student benefits from community-based arts education programmes range from social to academic. The issue is that music education, like other arts programmes, is frequently not taught in special education classrooms. We propose fifth-generation (5G)-based music education for cognitive computing for tailored behavioural intervention utilizing 5G in this article. Cognitive computing is a multidisciplinary sector of study that aims to develop computational designs and decision-making procedures depending on brain neurobiology, cognitive science, and psychology. Cognitive-behavioral techniques aim to change students' perceptions, alter their behaviour, and increase their pain control. Music and other cognitive therapies try to regulate the stress response by effectively mediating emotional responses. These objectives can be met by using behavioural treatments that influence the individual's affective response, which is influenced by prior experiences, prior knowledge, and emotions. The suggested system's performance is evaluated and compared to existing approaches.
EFFECT OF IMPLEMENTING NURSING CARE GUIDELINES ON NURSES KNOWLEDGES AND PRACTICES REGARDING EXTERNAL FIXATION IN ORTHOPEDIC PATIENTS
Authors:   Assistant Lecturer, Department of Medical Surgical Nursing, Faculty of Nursing, Kafrelsheikh University, Egypt., Sakr Ali Abdelhady Shaimaa , Ahmed Hassanien Amira ,   Professor, Department of Medical Surgical Nursing, Faculty of Nursing, Mansoura University, Egypt., Mohamed Hamed Hany ,   Assistant Professor, Department of Orthopedic Surgery, Faculty of medicine, Kafrelsheikh University, Egypt., Hassan Nabih Mohamed Madiha ,   Assistant Professor, Department of Medical Surgical Nursing, Faculty of Nursing, Mansoura University, Egypt.
Published: May 2022

Background:Pin site infections are a major hazard of external fixation that can be extremely stressful. Nurses are vital in the prevention of pin site infections. Aim:to assess the effect of implementing nursing care guidelines on nurses knowledges and practices regarding external fixation in orthopedic patients. Method:a quasi-experimental research approach was used. Sample:A convenient sample of 40 nurses from the orthopaedic departments of Kafrelsheikh University Hospital and a general hospital was used. Tools:Data were taken using two tools: the first was a self-administered questionnaire sheet, which included two parts: part 1 covered demographic data of the nurses and part 2 included nurses knowledge of the care provided to patients with external fixation. The second tool was an observation checklist for nurses to use before and after external fixation.Results:after implementing the guidelines there were significant statistical increase in nurses knowledge and practice (p0.001), as well as a statistically significant relationship between nurses educational level and their knowledge and practice (p=0.016, 0.001, respectively). Conclusion: The use of the developed nursing care guidelines was efficient in increasing nurses knowledge, preoperative practice, and postoperative practice while caring for patients with external fixation. Recommendations:provide nurses with ongoing training courses.
CASE REPORT: VAGINAL LEIOMYOMA
Authors:   Associate Professor, Department of Obstetrics & Gynaecology, Mahatma Gandhi Medical College & Hospital, Jaipur, India., Tiwari Kalpana , Bhardwaj Farendra ,   Associate Professor, Department of Obstetrics & Gynaecology, Mahatma Gandhi Medical College & Hospital, Jaipur, India., Gupta Shagun ,   Associate Professor, Department of Obstetrics & Gynaecology, Mahatma Gandhi Medical College & Hospital, Jaipur, India., Juneja Umang ,   Senior Resident, Department of Obstetrics & Gynaecology, Mahatma Gandhi Medical College & Hospital, Jaipur, India., Prabha Tushar ,   Assistant Professor, Department of Radio diagnosis, Mahatma Gandhi Medical College & Hospital, Jaipur, India., Patel Harsh ,   2nd Year Resident, Department of Obstetrics & Gynaecology, Mahatma Gandhi Medical College & Hospital, Jaipur, India.
Published: May 2022

Leiomyoma are benign, monoclonal tumors of the smooth muscle cells of the myometrium and contain large aggregations of extracellular matrix composed of collagen, elastin, fibronectin, and proteoglycan. [1] They are the most common benign tumors in females and are most frequently seen in uterus. Vaginal leiomyomas are very rare and often confused with a variety of vaginal tumors. Since the first detected case back in 1733 by Denys de Leyden approximately 300 cases have been reported in the literature so far. [2] They may present with varied symptoms depending on site, size and vascularity including lower abdominal pain, low back pain, vaginal bleeding, dyspareunia and urinary symptoms like frequency, dysuria or other features of urinary obstruction. Here we discuss a case of a 35 year old female who presented to us with complains consistent with prolapse and was provisionally diagnosed as paraurethral vaginal mass which turned out to be vaginal fibroid.
STRUCTURAL EQUATION MODELING ANALYSIS STUDENT SATISFACTION AND LOYALTY
Authors: Sibarani Riama , Santoso Teguh
Published: May 2022

This study aims to analyze and test the structural equation model of student satisfaction and loyalty as well as analyze and examine the influence of factors that influence student satisfaction and loyalty. The variables in this study are the image of the university, lecturer competence and service quality, student satisfaction and loyalty. This study also examines and analyzes the direct and indirect effects of the dependent variable on the independent variable of student satisfaction and loyalty as a mediating variable. The sample in this study were USNI students. The number of respondents who participated in this study were 370 respondents. Based on data analysis, the confirmatory factor that the value of the university image indicator, lecturer competence and service quality is greater than 0.5 is valid to reflect the latent variable. The results of the overall model fit test using the X2 (chi-square) test obtained a value of 2133.54, p-value of 0.000 and RMSEA = 0.076. Based on the model suitability test, testing the research hypothesis through the structural model, the results showed that the image of the university, lecturer competence and service quality had an effect of 89% on student satisfaction. In the second model, higher education institutions, lecturer competence, service quality and satisfaction have an influence of 82% on student loyalty.
RUSSIAN UKRAINE WAR ,WORLD REACTION AND ITS IMPACT ON THE INDIAN ECONOMY : A REVIEW
Authors:   Head Department of Zoology Govt .Degree College Doda, Jammu and Kashmir., Ahmed Wani Ajaz , Krishan Manhas Kewal ,   Department of Education Govt.Degree College Doda, Jammu and Kashmir., Ajaz Shaista ,   Govt .PG Women College Ghandi Nagar Jammu, Jammu and Kashmir.
Published: May 2022

In a blowing act of agression, Russian president Vladimir Putin launched a military assult on Ukraine on 24th of Feb.2022.The western leaders had warned of this possibility for weeks did little to cushion the shock.President Putin announced what he characterised as a special military operation to demilitarise and denazify Ukraine and made a barely coded threats of nuclear strikes upon any out side power that might come to its aid.Russia bombard the Ukraine as its troops entered from north, west and south.Whole world condemned the unprovked invasion on Ukraine.This war leads to continued fall of Indian rupee and condition can became more worse if the war continues and inflation spikes beyond the RBI target and input cost surge.
AUTOMATION OF DRUG DISPENSING: FEEDBACK FROM A HOSPITAL PHARMACY
Authors:   Mohammed V University of Rabat, Faculty of Medicine and Pharmacy, Rabat, Morocco., Wafaa Enneffah , Wartiti Mohammed Adnane El ,   Mohammed V University of Rabat, Faculty of Medicine and Pharmacy, Rabat, Morocco., Badr Adouani ,   Mohammed V University of Rabat, Faculty of Medicine and Pharmacy, Rabat, Morocco., Yasmina Tadlaoui ,   Mohammed V University of Rabat, Faculty of Medicine and Pharmacy, Rabat, Morocco., Ahmed Bennana ,   Mohammed V University of Rabat, Faculty of Medicine and Pharmacy, Rabat, Morocco., Nawal Cherkaoui ,   Mohammed V University of Rabat, Faculty of Medicine and Pharmacy, Rabat, Morocco., Jamal Lamsaouri ,   Mohammed V University of Rabat, Faculty of Medicine and Pharmacy, Rabat, Morocco.
Published: May 2022

Background: Ensuring drug circuit security is the ultimate goal for hospital pharmacists. Alarmed by the excessive drug consumption at its on-duty pharmacy, Mohammed V Military Teaching Hospital of Rabat (Morocco) installed a centralized automation for drugs dispensation. This experience is expected to be decentralized to all care units. Our study purpose is to evaluate automations impact on pharmaceutical drug management, and then to determine limits to be improved. Resources and procedures: We analyzed, over a period of 10 months, data from the database of the automated distribution system deposited at our on-duty pharmacyin order to determine its usage profile. We also evaluated through a questionnaire the point of view of our 13 pharmacy technicians toward drug dispensing automation. Results: Over the study period, we got the following results: - 5446 transactions were accomplished and 8864 drugs unit were dispensed - Parenteral route drugs are the most delivered (67%) - Anti-inflammatories and analgesics are the most required (26%), followed by the anti-infective drugs (25%) - Young technicians did more transactions (63%) than the elders did (37%) - According to pharmacy technicians, main automations advantages are saving time in locating medications (8/13) and limiting drugs errors (5/13). Otherwise, main constraints are the irregular machine replenishment (10/13) and the reduced capacity for storing all medications (6/13), specially refrigerated and oversized ones. Conclusion: Automation of drug delivery offers many advantages. However, there are still limits to be improved before its decentralization to all care units.
ELECTRONEUROMYOGRAPHIC AND CLINICAL ASPECTS OF BRACHIAL PLEXUS INVOLVEMENT IN PANCOAST-TOBIAS SYNDROME: A CASE REPORT
Authors:   Neurology Department, Mohammed VI University Hospital, Marrakesh, Morocco., Bentahar Hasna , Chraa Mohamed ,   Neurology Department, Mohammed VI University Hospital, Marrakesh, Morocco., Kissani Najib ,   Neurology Department, Mohammed VI University Hospital, Marrakesh, Morocco., Louhab Nissrine ,   Neurology Department, Mohammed VI University Hospital, Marrakesh, Morocco.
Published: May 2022

Pancoast-Tobias syndrome is a rare entity, defined by a clinical triad of cervicobrachial neuralgia in the C8-D1 territory, hand amyotrophy and Claude-Bernard-Horner syndrome, secondary to a tumor of the pulmonary apex. However, there are incomplete or misleading forms that can lead to a wrong diagnosis. The purpose of our observation is to study the clinical and electroneuromyographic patterns of brachial plexus involvement in Pancoast-Tobias syndrome.