bluuebunny/crossref_metadata_2025 huggingface dataset:
Influences of the Air Layer with Irregular Shape to the Absorbing Constructions
Authors: Liu Kai Hua , Lai Rong Ping , Chou Chuan Wen
Published: September 2011

Air layer with irregular shape in sound absorbing structure is formed by different structure mode of materials. With building multiform interior space by materials and structure mode, it makes the shape of air layer between the facing and the structure of building to be irregular shape.According to related study of absorbing structure, it shows less information about the influence of air layer with irregular shape. The factors of sound absorption of absorbing structure were focused on absorbing structure which facing paralleled structure of building in past research. For searching the influence of sound absorption of absorbing structure caused by the air layer with irregular shape, the subject in this study is set as the air layer with irregular shape which facing tilts with single-axis. The factors of air layer with irregular shape are the angle between tilting facing and horizontal face, the length of span of tilting facing, and if the setting is that the air layer is divided into several parts not to be interlinked. By these factors the sound absorption characteristics of air layer with irregular shape are shown.In the other point of effects of absorption coefficient causing by setting of air cavity, both panel and perforated panel structure the influences are influenced mainly at low frequency, especially at 200 Hz. Whether the air cavity is set or not, the panel structure reveals less influences and the absorption coefficient reduces as increasing of span at low frequencies.
Thiacalix[4]Arenetetrasulfonate Applied to Remediation of Soils Contaminated with Lead
Authors: Zhao Da , Sun Tie Heng , Hu Xiao Jun , Hu Xiao Min
Published: September 2011

The performance of thiacalix[4]arenetetrasulfonate (TCAS) for the treatment of soils contaminated with lead was evaluated in this study. Batch experiments were performed to test the factors influencing remediation of lead-polluted soil. The effects of temperature, solid-liquid ratio and the ionic strength on the removal capacities of lead by TCAS reagent had been studied. The results showed that the TCAS reagent was effective for removal of lead from the contaminated soil, the optimum temperature of TCAS for soil treatment was 5°C-25°C. The optimum solid-liquid ratio was 1:10 for soil treatment. Adding Ca(NO3)2 was of no advantage for lead removal, and the effect of Ca(NO3)2 on the removl rate was less when its molarity was less than 0.01mol/L. This experimental work has also demonstrated the great importance of soil matrix for the evaluation of the TCAS leaching as a cost effective remedial option.
Anti-Tumor Immunotherapy Elicited by Cross-Link Peptide Vaccine Alpha-Fetoprotein and Heat Shock Protein 70 Complex
Authors: Lin Huan Ping , Wang Xiao Ping , Wang Qiao Xia
Published: September 2011

Purposes: To construct a recombinant vaccine alpha-fetoprotein (AFP)-heat shock protein (HSP70) complex, and study its ability to induce specific CTL response and its protective effect against AFP-producing tumor. Material/Methods: A recombinant vaccine was constructed by conjugating mouse alpha-fetoprotein to heat shock protein 70. By way of intracutaneous injection, mice were primed and boosted with recombinant vaccine mAFP/HSP70, whereas single mAFP or HSP70 injection as controls. The ELISPOT and ELISA were used to measure the frequency of cells producing the cytokine IFN-gama in splenocytes and the level of anti-AFP antibody of serum from immunized mice respectively. In vivo tumor challenges were carried out to assess the immune effect of the recombinant vaccine. Results: By recombinant mAFP/HSP70 vaccine immunization, the results of ELISPOT and ELISA showed that the number of splenic cells producing IFN-gama and the level of anti-AFP antibody of serum were significantly higher in mAFP/HSP70 group than those in mAFP and HSP70 groups (108.50±11.70 IFN-gama spots/106 cells vs 41.60±10.40 IFN-gama spots/106 cells, 7.32±3.14 IFN-gama spots/106 cells, P<0.01; 156.32±10.42 µg/mL vs 66.52±7.35 µg/mL, 5.73±2.89 µg/mL, P<0.01). The tumor volume in mAFP/HSP70 group was significantly smaller than that in mAFP and HSP70 groups (42.44±7.14 mm3 vs 392.23±12.46 mm3, 838.63±13.84 mm3, P<0.01). Conclusions: The study further confirmed the function of heat shock protein 70’s immune adjuvant. Sequential immunization with recombinant mAFP/HSP70 vaccine could generate effective antitumor immunity on AFP-producing tumor. The recombined mAFP/HSP70 vaccine may be suitable for serving as an immunotherapy for hepatocellular carcinoma.
Comparison of Growth Hormone Pulsatile Secretion and Serum Metabolites in Growing Taihu and Landrace Pigs
Authors: Li Guo Wang , Miao Zhi Guo , Wang Shi Zhu , Liu Chang Zhong , Zhang Hai Tang
Published: September 2011

In this paper we compared growth performance, growth hormone (GH) pulsatile secretion and serum metabolites in growing Taihu and Landrace gilts at the same age. The results showed that compared with Landrace gilts, Taihu gilts decreased by 45.48% (P<0.05), 25.25% (P<0.05) in average daily gain and carcass muscle percentage, and increased by 52.44% (P<0.05), 69.50% (P<0.05) in feed/gain ratio and carcass fat percentage, respectively. While, average daily feed intake was not affected by breeds. Serum basal GH level, mean GH level and GH pulse amplitude were decreased by 26.67% (P<0.05), 20.00% (P<0.05) and 18.81% (P<0.05), respectively, but GH pulse frequency and pulse duration remained unchanged (P>0.05). Serum urea nitrogen concentration in Taihu gilts was 32.04% (P<0.05) higher, whereas, total protein level was 17.73% (P<0.05) lower than that of Landrace gilts.
Emulsifier’s Influence on the Quality of Frozen Dough
Authors: Bao Yu Ru , Wang Xian Lun , Ren Shun Cheng
Published: September 2011

The study focused on the effect of four emulsifier ssl-csl, three monoesters, the single glyceride, the sugar ester for the quality of frozen dough steamed bread. We found the best percentage of additives through a single factor experiment and orthogonal experiment .The results showed that: the best formula was 0.2% ssl-csl, 0.25% three monoesters, 0.25% single glycerides, 0.5% sucrose ester. This compound emulsifier can improve the toughness of steamed bread, all indications were ideal.
Antioxidant Activities of Extracts from Pholiota Nameko
Authors: Ji Hong Fang , Zhang Ling Wen , Zhang Hai Yan , Li Guang Lei , Yang Ming Duo
Published: September 2011

Holiota nameko is an edible mushroom widely cultivated in the northeast of China. The cold water, hot water and ethanol extracts were prepared and their antioxidant properties were studied. At 2 mg/mL, the cold water extract showed a high reducing power of 1.502, whereas those of hot water and ethanol extracts were 0.868 and 0.159. With regard to the scavenging ability on DPPH free radicals, extracts were effective in the order: cold water > hot water > ethanol extracts. EC50 values for chelating ability on ferrous ions for cold water, hot water, and ethanol extracts were 1.461, 0.663, and 11.791 mg/mL, respectively. Based on EC50 values, the water extracts of P. nameko were effective antioxidants.
Anti-Melanogenic Activities of Collagen Peptides from Jellyfish (Stomolophus meleagris)
Authors: Zhuang Yong Liang , Sun Li Ping
Published: September 2011

Collagen was extracted from jellyfish (Stomolophus meleagris) and hydrolyzed to prepare collagen peptides. Three fractions (JCP-1<1kDa, 1kDa3kDa) were collected by ultrafiltration from jellyfish collagen peptides and inhibitory abilities of melanogenesis were evaluated using cultured B16 melanoma cells. The results showed three fractions could inhibit intracellular tyrosinase activity, decrease melanin content and enhance glutathione synthesis. Furthermore, they decreased intracellular cAMP levels and suppressed tyrosinase mRNA expression. JCP-2 showed much higher anti-melanogeneic activities than JCP-1 and JCP-3 because of its amino acids composition and molecular weight distribution. Our results indicated JCP-2 could be used as a natural skin-lightening agent in the medicine and food industries.
Growth Inhibition of Kappa-Selenocarrageenan Combined with Paclitaxel on Human Breast Carcinoma MCF-7 Cells
Authors: Ling Na , Chen Chun Ying , Xu Yan Yan , Ji Yu Bin
Published: September 2011

To investigate the combined effect of kappa-selenocarrageenan (KSC) and paclitaxol (Taxol) on MCF-7 cells in vitro. The cytotoxic effect of both drugs on MCF-7 cells was measured by MTT assay, and the King’s principle was used to evaluate the interaction of drugs combination. KSC and Taxol alone have significant growth inhibitory effects on HepG-2 cells in dose- and time-dependent manners, and the combination groups show more remarkable anti-proliferative activities than the two drugs alone. The IC50 values of KSC and Taxol alone on HepG-2 cells for 72h are 48.445 mg/L and 2.592 nmol/L, respectively. While combining with 30 mg/L KSC, IC50 value of Taxol decreases sharply to 0.353 nmol/L. Q values of their combination are 0.908~1.051, which demonstrates additive effect. In short, selenium polysaccharide has striking inhibition effect on MCF-7 cells and can enhance efficacy of chemotherapeutic agents and reduce their side effects.
Isolation of a Field Marek’s Disease Virus with Acute Oncogenicity from Tibetan Chickens in China and Sequence Analysis of Oncogenic Genes
Authors: Tian Ming Xing , Deng Rui , Zhao Yang , Li Min , Cao San Jie , Wen Xin Tian , Huang Yong
Published: September 2011

A field Marek’s disease virus (MDV), named as BY strain, was firstly isolated from Tibetan chickens in Sichuan province, China, by method of co-cultivation of the lymphocytes with duck embryo fibroblasts (DEF). Analysis of the oncogenic genes showed that there were 2 copies of 132-bp repeated sequence in long terminal repeat of the BY strain, The nucleotide and amino acid sequence identities of Meq gene of BY strain with other prevalent MDV strains in China were 97.6-100.0% and 98.8-100.0%, respectively, and some point mutations assumed to be relevant to the oncogenecity of MDV also existed in the Meq gene of BY strain. The result of animal challenge test on specific-pathogen-free (SPF) chickens showed lymphomas may occur in a variety of organs as early as 18 days post challenge, and the rate of tumor occurrences and mortalities reached to 73.33% and 66.67% in HVT immunized chickens, respectively. In conclusion, an MDV strain charac-terized of acute oncogenicity was isolated from Tibetan chickens in China, though there were no obvious difference between the oncogenic genes of this strain and other virulent MDV strains isolated in China in recent years.
Bioconcentration and Elimination Kinetics of Copper with Different Tissues of the Ascidian Styela Clava
Authors: Jiang Ai Li , Yu Zhen , Wang Chang Hai
Published: September 2011

Based on the semi-static two compartment model, the bioconcentration and elimination of copper with tissues of Styela clava were investigated. The kinetic parameters (accumulation rate constant k1, elimination rate constant k2, bioconcentration factor BCF, biological half life t1/2 and maximum equilibrium concentration CAmax) were obtained by non-linear regression. The results showed S. clava could accumulate copper from the aquatic environment and that BCF decreased with increasing metal concentration in water. When the accumulation achieved balance, CAmax showed positive correlation to metal concentrations in water. Concentrations of copper in different tissues of S. clava were in the order of gonad > digestive gland ≈ other part >tunic, and t1/2 of copper was 12.57 to 38.18 days in elimination phase. The high rate to accumulate and eliminate copper from its body exhibited a useful potential biomonitor of short-term copper fluctuations status in marine system.
Optimization of L-Arabinose and Single-Cell Protein Production from Corn Husks Hydrolysate
Authors: Du Li Ping , Gai Wei Dong , Xiao Dong Guang , Liu Lei , Hao Rui Xue
Published: September 2011

Glucose, xylose and L-arabinose were released from corn fiber by dilute acid hydrolysis. The ability of a Candida tropicalis strain to ferment glucose and xylose in corn fiber hydrolysates to produce single cell protein has been examined. The crystalline L-arabinose can be prepared from the fermentation supernatant. The L-arabinose retention rate and biomass protein production by the yeast grown on corn fiber hydrolysate were studied under various culture conditions. The optimal volume of the broth, inoculum size, temperature, initial pH and shaker speed for retention rate of L-arabinose and growth of the strain were 30ml broth in 250ml capacity flask, 4% (v/v), 26°C, 4.0 and 180r/min, respectively. Retention rate of L-arabinose was increased by such conditions to 89.26% and cell dry mass to 13.9g/L, respectively. These values corresponded with high biomass protein and crystalline L-arabinose.
PCR-Based Detection of Traces of Potentially Allergenic Soybean (Glycine max) in Food Matrices
Authors: Wang Bang Ping , Li Zhen Xing , Pawar Ravindra , Wang Xiao Fei , Lin Hong
Published: September 2011

As an important source of food ingredients, it is necessary for soybean detection in foods because it was reported as one of the “big 8” food allergens. In this report, a PCR-based method was developed enabling the detection of even small traces of potentially allergenic soybean traces in food products. Soybean-specific primer was designed based on Gly m Bd 28K, one of the major allergens in soybean. The assay was applied to a wide range of food matrices and the detection limit was 0.01% (100ppm) for spiked peanut and whole milk samples, while it was 0.001% (10ppm) for skim milk, wheat and mung bean samples. It seems enough for soybean allergen detection in food matrices according to the threshold of soybean.
Application of PE Pipe in Underground Service Pipe
Authors: Wang Xing Wei , Peng Shi Ni , Huang Xiao Mei
Published: September 2011

The problems with underground service pipes are the regular session of accidents in city gas line network which is directly related to people's lives. However, there are potential risks with underground service pipe. The scientific and reasonable gas service pipe should be to minimize the accidents and easy to installed and guarantee the economical in the case of ensuring gas use. Comparing and analyzing the two ways frequently used in daily life from the aspect of pipe materiel and pipe laying methods, drawbacks of them are identified. Finally, the main use of PE pipe is recommended and measures to transform are proposed, which eliminate the security risk after improvement, and obtain the economic benefit in the long term.
The Function of Chloroplast GST of Puccinellia Tenuiflora Seedling Leaves in Resistance to Na2CO3 Stress
Authors: Sun Guo Rong , Wu Xue Li , Chen Gang , Wang Jian Bo , Cao Wen Zhong , Du Qun , Zhang Biao
Published: September 2011

In order to probe into orderliness changes of Glutathione S-Transferase (GST) of chloroplast ofPuccinellia tenuifloraseedlings under Na2CO3stress and its function in resistance to Na2CO3stress, relative electric conductance, GST activity and the O2-.produce rate of the chloroplast, and the osmotic potential of leaves, and the osmotic potential of culture solution ofP. tenuifloraseedlings under different Na2CO3stress were concerned. The result shows that in the Na2CO3stress range of 0～0.4%, along with the increase of its intensity under different Na2CO3stress intensity, GST activity of the chloroplast of seedling leaves ofP. tenuiflorais strengthened and GST activity is rapidly weakened with the increase of the intensity of Na2CO3stress more than 0.4%. The change of GST activity of the chloroplast along with the osmotic potential of culture solution and seedling leaves, relative electric conductance of the seedling leaves as well as the O2-.produce rate have the similar change tendency. There is significant nonlinear relationship among GST activity of chloroplast, osmotic potential of the seedling leaves and Na2CO3concentration ofculture solution, and among GST activity of chloroplast, O2-.produce rate and osmotic potential of seedling leaves, and among GST activity of chloroplast, osmotic potential of culture solution and that of seedling leaves, and among GST activity, the O2-.produce rate of chloroplast and relative electric conductance of the seedling leaves. These indicate that GST of chloroplast plays an important role in the process of seedlings ofP. tenuiflorain resistance to the low intensity of Na2CO3stress.
Phospholipase D-Mediated Transphosphatidylation in Subcritical 1, 1, 1, 2-Tetrafluoroethane
Authors: Zhang Hui , Yu Gang , Wang Yu Ming , Xue Chang Hu
Published: September 2011

Phospholipase D-mediated transphosphatidylation to phosphatidylserine from phosphatidylcholine was successfully performed using subcritical 1,1,1, 2-tetrafluoroethane (R134a) as reaction medium. Phospholipase D from Stretomyces sp was found to be active in subcritical R134a at 30-50°C and 2-8MPa; Higher velocity and conversion were observed with respect to those attained in conventional organic solvents. A maximum conversion of 84.88% was attained at 6 MPa, 40°C, after 240 minutes with 9.6 units phospholipase D.
Effect of CuO Nanoparticles on the Cell Membrane Permeability of A549 and its Exclusion
Authors: Xie Xiao Yan , Xu Li Li , Gao Dong Mei
Published: September 2011

This study was conducted to explore the effect of CuO nanoparticles (NPs) on the cell membrane permeability and its exclusion from cells. Human A549 lung cells were exposed to 5mg/L and 15mg/L CuO NPs. Cell membrane permeability was evaluated in 2h and 4h. After 4 hours exposure, the membrane was damaged. Exclusion of copper from cells after 24h exposure with 5mg/L and 15 mg/L CuO NPs are time and dose dependent. And the cell viability was resumed gradually. It is concluded that CuO NPs could induce cytotoxicity, and destroy the membrane integrity. One detoxify mechanism was the exclusion of excessive copper from cells themselves.
A Novel Strain Pseudomonas Aeruginosa CS-2 Producing an Organic Solvent-Tolerant Lipase
Authors: Peng Ren , Lin Jin Ping , Wei Dong Zhi
Published: September 2011

Organic solvent-tolerant lipases have shown new potential application in biotechnological fields. A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China. Based on the morphological, physiological and biochemical features and 16S rDNA sequence of the strain, it was identified asPseudomonas aeruginosaCS-2. The crude lipase had an improved stability in the presence of acetonitrile and it exhibited stability in presence of benzene, chloroform,n-Hexane, petroleum ether and isooctane. The optimized composition of culture medium was as follows: olive oil 1%, peptone 4%, K2HPO40.5%, MgSO4·7H2O 0.1%, gum arabic 0.04%. The optimum condition for lipase production was on the following: temperature 37°C, initial pH 7.5, rotary shaking speed 200 rev/min, culture time 48 h.
Evaluation of Oxidative Stress Responses and Neurotoxicity Potential of Methamidophos in Mytilus Edulis
Authors: Yu Zhen , Jiang Ai Li
Published: September 2011

We investigated the oxidative stress of methamidophos onMytilus edulis. Sublethal concentrations of methamidophos (5.0, 10.0 and 15.0 μg L-1) were administered toM. edulisfor 5, 15 and 30 days respectively. The biochemical stress response ofM. eduliswas studied taking into account acetylcholinesterase (AChE), Na+-K+-adenosine triphosphatase (Na+-K+-ATPase) and some antioxidant enzyme activities. Protein contents in tissues was also measured. With the increase of methamidophos concentrations the activities of AChE and Na+-K+-ATPase in tissues decreased, whereas the activities of GPx, SOD and CAT increased. The protein content in tissues decreased after 5 days and then gradually increased until the 15th day of the experiment. On the 30th day, the protein level in organism was observed to have reached to that of the control group. It can be stated that methamidophos exerted its effect even at low concentration , its toxicity increased depending on its dosage and exposure time. This study revealed thatM. edulisdeveloped adaptive response to neutralize the oxidative stress following pesticide exposure.
Application of Two Modeling Methods in Optimization for Adenosine Extraction from Mycelium of Cordyceps Militaris
Authors: Du Lin Na , Song Jia , Meng Ling Jun , Lu Jia Hui , Meng Qing Fan , Teng Li Rong
Published: September 2011

An experimental mixture design coupled with data analysis by means of both response surface methodology(RSM) and artificial neural network(ANN) was applied to explore the optimum process parameters for adenosine extraction from cultured mycelium of Cordyceps militaris. With the extraction rate of adenosine as index, the critical factors selected for the investigation were extracting temperature, extracting time and solid-liquid radio. The results obtained by the application of ANN were more reliable since better statistical parameters were obtained. The optimum extraction procedure was as follow: extracting time 2.3 h, extracting temperature 48 °C, solid-liquid ratio 1:38 g⋅mL-1. Under the optimal conditions, the corresponding response value predicted for adenosine production was 4.59 mg g-1, which was confirmed by validation experiments.
Photocatalytic Inactivation of the Bacteria Escherichia Coli by CuCr2O4/TiO2 Composite Photocatalysts under Simulated Solar Light Irradiation
Authors: Zhang Li , Yan Jian Hui , Huang Ke Long , Chen Hao
Published: September 2011

CuCr2O4nanoparticles have been successfully synthesized via a facile citric acid (CA)-assisted sol-gel method, and further combined with TiO2by solid phase method. These obtained photocatalysts were characterised by X-ray diffraction (XRD) and UV-vis diffuse reflectance spectrum (UV-vis DRS). Then the photocatalytic inactivation ofEscherichia coli, a Gram-negative bacterium, was performed with the photocatalysts to investigate the photocatalytic bactericidal capability under simulated solar light irradiation. The results show that CuCr2O4/TiO2composite photocatalysts exhibited a much better photocatalytic activity than either pure CuCr2O4or TiO2, and in the composites, the effects of CuCr2O4to TiO2mass ratio and calcination temperatureon bacterial photocatalytic inactivation were investigated in detail. With a 90% content of TiO2, calcination temperature at 500◦C, the optimal photocatalytic sterilization amount of CuCr2O4/TiO2is 1.62 and 1.33 times more than that of CuCr2O4and TiO2,respectively. AndEscherichia coliwere almost completely inactivated within about 40 min.
Determination of Eight Major Bioactive Components in the Traditional Chinese Medicinal Preparation Xiaochengqi Decoction by High Performance Liquid Chromatography
Authors: Li Kang , Wang Yuan Yuan , Bi Yong Guang
Published: September 2011

High performance liquid chromatography was employed to determine the contents of eight marker bioactive components such as rhein, aloe-emodin, nobiletin, emodin, honokiol, magnolol, chrysophanol, and physcion in the traditional Chinese medicinal preparation, Xiaochengqi decoction. The separation was performed on a C18 column by stepwise gradient elution with water (1.1% v/v acetic acid)–acetonitrile (0.01min, 75:25; 15 min, 60:40; 30 min, 50:50; 40 min, 45:55; 55min, 30:70) as the mobile phase at a flow-rate of 1.0ml·min-1, with UV detection at 254nm. Eight regression equations showed good linear relationships between the peak area and the amount of each marker. The recoveries of the markers were 99.6, 99.5, 97.4, 97.7, 98.9, 97.9, 99.0 and 98.5%, respectively. The repeatability and reproducibility (relative standard deviation) of the method were less than 2.1% and 2.5%, respectively.
A Cyclic Softening Plastic Model of Carbon Steel 45 under Uniaxial Cyclic Straining
Authors: Luo Yan
Published: September 2011

Based on the characteristic of cyclic softening of the quenched and tempered Carbon steel 45, a cyclic plastic constitutive model was proposed to describe the cyclic behavior under symmetrical and unsymmetrical strain cycling with different strain amplitudes. In this model, the phenomenon of the decrease of the up yield limit stress with the increase of strain in the initial 1/4 cycle was taken into account. The proposed evolution equations of the yield size and backstress can simulate the cycling softening under symmetrical and unsymmetrical strain cycling well. The results indicated that either the simulated shape of the cyclic softening hysteresis loops or the evolution of stress amplitude with the increase of the cyclic number during the low cycle fatigue coincides with the experimental ones very well.
The Security Analysis of Trace Elements Accumulated in Fish Tissues with the Aquatic Eco-Environment from Fresh and Marine Waters
Authors: He Jin Zhe , Yang Kai , Sun Pei Long
Published: September 2011

The pollution level of trace elements from nine types of fishes was study by ICP-AES techniques, and compare the pollution level differences of trace elements between fishes of fresh and marine waters. Assess the pollution risk of trace elements accumulated in fish tissues with the aquatic eco- environment from fresh and marine waters. The concentration changes of trace elements in fish tissues have some differences from freshwater fish tissues and marine water fish tissues, but the concentration distribution tendency of trace elements was similar. Mg elements had high concentration, Fe elements were rich in fish tissues, and Zn elements were in a relatively lower level in fish tissues. Their bioaccumulation levels with other trace elements, such as Mn, Cu, Ni, Co, Se, Cr, Mo, Sn and Al, were not considered as main contaminate elements in fish tissues, the pollution level of toxic elements like Cd, Pb and Hg in most fish tissues from this study demonstrated that the cultivation aquatic eco- environments of fresh water and marine water were relatively safe. But the potential pollutions of toxic elements in fish tissues, such as Cd, Pb, As and Hg should pay main attention.
Cyanobacteria Diversity in Eutrophic Lake of Yunnan, China
Authors: Liu Li , Xiang An , Feng Yue , Wei Da Qiao , Yang Heng , Xia Xue Shan
Published: September 2011

Cyanobacteria are widespread in eutrophic freshwater lakes and can produce potent toxins which pose serious risk for human and animal health, aquatic ecosystem sustainability and economic vitality. There are 9 major lakes, which eutrophication and related ecological problem had occurred in most of these lakes in Yunnan, China. In this study, water samples were collected at different sampling time from five freshwater plateau lakes, which located in the middle area of Yunnan province, to assess the cyanobacteria diversity vary with the seasons. The morphological character of cyanobacteria were observed by light microscope (LM), the genetic diversity of cyanobacteria had been demonstrated by constructed phylogenetic trees based on DNA sequence of cyanobacteria 16S-23S rRNA internal space (ITS).The results showed that a variety of cyanobacterial species were distributed in five plateau freshwater lakes. The cyanobacterial population consisted mainly of several Microcystis species as dominants in Dianchi Lake and the dominant species of cyanobacteria found in June to October of 2009 was Microcystis aeruginosa. The diversity of cyanobacteria in Caohai was higher than that of Waihai in Dianchi Lake.
Study on Image Mosaic and Registration of Electronic Component through Surface Mounting Technology
Authors: Li Xi Bing , Bao Li , Wang Shi Gang , Zhang Hong Xia , Guo Jian Hua , Li Jing Hui , Hu Qing Ming , Han Gang , Fan Yan Bin , Liu Xian Li
Published: September 2011

The rapid growth of modern technology place greater demand on electronic products’ performance and quality. As for the printed circuit board (PCB) assembly of electronic components through surface mounting technology (SMT), high-pixel charge coupled device (CCD) can only screen local images during image collection because of its limited vision. A rapid image mosaic technology has been proposed to seamlessly stitch the local images with overlapping boundaries. The complete image of PCB assembly has been acquired through rapid area matching method within double Mark points and Mark circle. The experiment results show that this method can greatly reduce the image-mosaic time and fairly improve the real-time performance of modern automatic optical inspection (AOI) equipment. Furthermore, such method avoids the alignment error from the characteristic points for noise jamming or noise shielding, and therefore obtains better robustness.
Reagentless near-Infrared Determination of Cholesterol in Undiluted Human Serum Using Interval Partial Least Square
Authors: Gao Hong Zhi , Lu Qi Peng , Huang Fu Rong
Published: September 2011

In order to determination of cholesterol in human serum with no reagent using near-infrared (NIR) spectroscopy. Interval partial least square (iPLS) was proposed as an effective variable selection approach for multivariate calibration. For this purpose, an independent sample set was employed to evaluate the prediction ability of the resulting model. The spectrum was split into different interval. Then, the informative region of cholesterol (1688-1760nm), in which the PLS model has a low RMSEP with 0.241mmol/L and a high R with 0.975, is selected with 23 intervals. The results indicate that, the informative region of cholesterol can be obtained by iPLS and applied to design the simpler reagentless NIR instruments for inexpensive cholesterol measurement in future.
Separation of Angiotensin-Converting Enzyme Inhibitors from Fermented Milk Prepared with Lactobacillus casei HZ1
Authors: Han Zhao , Luo Xue Gang , Tian Hua , Men Yan , Wang Nan , Jiang Yong , Zhang Tong Cun
Published: September 2011

In this paper, a strain HZ1 which was isolated in our former works was classified as Lactobacillus Casei (L. Casei) by 16S rDNA sequence analysis. The results of angiotensin-converting enzyme (ACE) inhibitory assay showed that this novel stain was able to produce ACE inhibitory components when it was used to fermented milk. Applying the methods of membrane filtration and anion-exchange chromatography with fast protein liquid chromatography (FPLC) system, nine fractions with remarkable ACE inhibitory activity were obtained, and two bioactive fractions, recorded as ACEI-2 and ACEI-5, exhibited the highest ACE inhibitory rate of 60.55% and 71.71%, respectively. Furthermore, seven fractions proteins/polypeptides, which exhibited ACE inhibitory rate higher than 50% were separated from ACEI-2 and ACEI-5 by using sephadex gel filtration chromatography. These works might provide a foundation for the following study of L. Casei HZ1 and its probiotics functions, and contribute to the development of functional foods or drugs that provide health benefits to patients suffering from hypertension.
Microbial Fermentation Extraction Processing of Protein from Soybean
Authors: Wu Hai Bo , Jiang Lian Zhou , Li Yang , Wu Hai Tao
Published: September 2011

Although hexane extraction of soybean oil has the high recovery，residual hexane in oil is noxious for consumer health. Crew pressing and extruding-expelling extraction processes denature protein to render functional properties poor for food and meal use. This processing employed microbial fermentation to extract protein from full fat soy flour so that denaturation degree of protein reduced. The results showed that low temperature flaking and extrusing soy flakes was beneficial to improving protein extraction, after optimizing the fermentation conditions with the Response Surface Methodology (RSM), total protein extraction yield achieved the highest value - 86.66% at optimal fermentation conditions - 6.6% extruded full fat soy flour, 5.4% inoculation volume, the initial pH8.1. These advances make the microbial fermentation extraction process of protein from soybeans attractive channel as the environmentally – friendly technology.
3-D Dynamic Analysis for Functionally Graded Material Plates with Different Openings
Authors: Yu You Zhen , Sun Yi Qun
Published: September 2011

The problems of functionally graded materials are supposed to be studied incorporating their microstructures. The microelement method proposed here is characterized by this capability. In this work, by using the Fortran source program of microelement method established on the basis of existing finite element method, 3-D dynamic analysis of functionally graded material plates with different openings is performed. It can be known from the numerical results that the shapes and number of openings have a significant influence on natural frequency and basic vibration modes of functionally graded material plates, which is different from the results of homogeneous plates. These results are significant to applications of functionally graded material plates with openings.
The Signal Pathways of Immune Inflammation Mediated by the Tlr3/Nf-Kappab and Activator Protein-1 in Cells Infected with Influenza A Virus Antagonized by Baicalin
Authors: Zhang Chun Jing , Yu Hai Tao
Published: September 2011

Baicalin has better anti-inflammatory function, antioxidant function and antiviral activity, but the mechanism of the antiinfluenza viral activity of baicalin has not been revealed.Toll-like Receptor 3 and the signal pathways mediated by TLR3 were affected and controlled by the infections with influenza A virus. We report here the significant activity and part mechanism of baicalin against H3N2 influenza A viruses. Baicalin could well protect the damages of cells caused by influenza A virus, it also could effectively inhibit the production of CPE in cells caused by influenza A virus and the inhibition of cells growth. The mechanism of antiinfluenza virus infection of baicalin may be related with the following aspects: to decrease the transcriptional activity of the oxidative stress sensitive transcription factor NF-kappaB and AP-1 by moderately decrease the higher expression level of TLR3 mRNA and the higher expression level of protein; and to further inhibit the mRNA expression of the downstream target genes IL-1β, IL-8, RANTES and IFN-β thereby alleviate the inflammatory injuries and restore the stability and balance of immune function in vivro.
Grx1 Antagonized High Glucose-Induced Apoptosis in Endothelial Cells through Inhibition of Jnk Pathway
Authors: Yu Hai Tao , Yue Li Ling , Zhang Chun Jing
Published: September 2011

To investigate the mechanism underlying the protective effects of glutaredoxin-1 (Grx1) against high glucose-induced apoptosis in umbilical vein endothelial cells. The proliferation of cells was measured by MTT assay. The cells ultra-structure were observed by TEM and the apoptotic rate was detected by the immunofluorescent of Annexin V－FITC/PI with flow cytometer. The level of p-JNK and JNK were evaluated by western bloting. Results showed that Grx1 prevented the inhibitory effect on cell viability induced by high glucose; Grxl could inhibit high glucose-induced apoptosis and restrain apoptosis rate of endothelial cell significantly. The expression level of p-JNK protein significantly increased while that of JNK protein has no insignificantly chang in cells of the high glucose group, After pretreatment with Grx1, the expression level of p-JNK protein decreased. These results demonstrated that Grx1 has protective effects against high glucose-induced apoptosis in HUVECs through inhibition of JNK pathway.
In Vivo Studies of the Toxicity of Multi-Wall Carbon Nanotubes
Authors: Liu Ai Hong
Published: September 2011

Carbon nanotubes (CNTs) may play a significant role in the biomedical field in future. Therefore, it is imperative to thoroughly investigate the toxicity and the biocompatibility of CNTs. However, the data about the toxicity of carbon nanotubes in vivo is poor or contradictory. In this study, rats were instilled with different dose mutil-wall carbon nanotubes (MWCNTs) by intravenous injection and euthanized 90d after the single treatment for histopathological study of the lungs and other organs. All rats survived within our experimental period and no large changes in their weights appeared. It is confirmed that all MWCNTs-exposed rats suffered multiple lesions. Obvious changes of the structure of lung tissue and the micrograph of MWCNTs in lung tissue were observed by TEM, while other tissues were in order. The results suggest that MWCNTs can induce lung toxicity in the expose modes of intravenous injection.
Applications of Quantum Dots in Cancer Research
Authors: Wang Xue Feng , Ding Jing , Li Ji Yu , Jiang Han , Wang Zi Hao , Shi Wei Bin
Published: September 2011

Quantum dots(QDs) usually refers to nanocrystalline materials whose diameter is smaller than the exciton Bohr radius. These materials have quantum size effect,the most significant manifestation is their optical properties change with particle size.The unique optical properties make quantum dots to be Ideal markers for tumor cell tracking and targeting,such as mammary cancer, liver cancer, and melanoma.There are broad prospects in tapping the potential of this highly sensitive technology in serum and other body fluids, so as to increase the early diagnosis rate of tumors.
Probing Near-Infrared Quantum Dots for Imaging and Biomedical Applications
Authors: Wang Zi Hao , Wang Xue Feng , Jiang Han , Ding Jing , Wang Jian Dong , Shi Wei Bin
Published: September 2011

As light-emitting nanocrystals, quantum dots (QDs) have created a new realm of bioscience by combining nanomaterials with biology. They also have been a major focus of research and development during the past decade, which will profoundly influence future biological as well as biomedical research. In recent years, near-infrared (NIR) quantum dots have emerged in analytical applications, especially for in vitro and in vivo imaging. The impetus behind such endeavors can be attributed to their unique optical and chemical properties, with size-tunable light emission, high photo stability, and manifold fluorescence colors. In this review, we focus on fluorescent imaging with near-infrared (NIR) quantum dots (QDs) both in vitro and in vivo, and the advantages of QDs and potential problems to their use in practical biomedical applications. The ultimate targets aim at decreasing the cytotoxicity of QDs and the future outlook of QD applications in biomedical fields.
The Preparation of Carbon Nanotubes (CNTS) Solid Phase Microextraction (SPME) Probe and Application on the Analysis of Octylphenol and Nonylphenol in Water Coupled with Gas Chromatography-Mass Spectroscopy (GC-MS)
Authors: Zheng Yong Jie , Sun De Zhi , Tian Jing Zhi
Published: September 2011

In this paper, the carbon nanotubes (CNTs) solid phase microextraction (SPME) probe was prepared and characterized. The octylphenol and nonylphenol in the water were determined by the probe coupled with the GC-MS method. The factors which impact extraction efficiency such as extraction temperature, extraction time, sample dilution multiple and ionic strength were optimized. A pulse stress of carrier gas was applied to the inlet of GC column to improve the determination sensitivity of GC-MS. The method linear ranges of octylphenol and nonylphenol were from 0.01μg·L-1 to 20μg·L-1, and the related coefficient was 0.992 and 0.996. The recoveries were from 91.89% to 92.01%, detection limits were from 0.01μg·L-1 to 0.02μg·L-1, and relative standard deviations (RSDs) were from 3.2% (n=5) to 4.1% (n=5).
The Hydrogen Production of Mixed Acid Fermentation in Bio-Hydrogen Producing Reactor
Authors: Qin Zhi , Ren Nan Qi , Li Jian Zheng
Published: September 2011

Hydrogen gas was obtained from organic wastewater by acidogenic fermentation of two-phase anaerobic wastewater treatment process. In this paper, the hydrogen production of mixed acid fermentation was investigated. In mixed acid fermentation, pH value of fermentation was in the range of 4.5~5.0, the COD removal rate was about 20%. The average transferring rate of sugar was about 94.6%. Our results demonstrated that when Organic Loading Rate was 15 kgCOD/m3·d, the specific biogas production rate of mixed acid fermentation was 1834~2392 mmol/(kg∙d), and the average specific biogas production rate was about 2077 mmol/(kg∙d). The specific hydrogen production rate of mixed acid fermentation was about 532~710 mmol/(kg∙d), and the hydrogen content was around 30%. The main liquid products of our experiments in mixed acid fermentation were ethanol, acetic acid and propionic acid.
Numerical Simulation of Inversion Population Distribution in Diode-End-Pumped Quasi-Three-Level Laser
Authors: Song Zhi Zhang , Li Qin An , Zhang Tao
Published: September 2011

In this paper, the inversion population distribution model in the gain medium are given and simulated numerically corresponding to different cavity conditions for the continuous wave diode-end-pumped quasi-three-level solid-state laser.
Preparation, Characterization, and In Vitro Evaluation of Resveratrol-Loaded Bovine Serum Album Nanoparticles
Authors: Li Jia Lei , Zu Yuan Gang , Zhao Xiu Hua , Zhao Dong Mei , Chen Xiao Qiang , An Zhi Gang , Wang Kun Lun
Published: September 2011

Resveratrol (RES) is a naturally occurring triphenolic phytoalexin compound exerting numerous beneficial effects in the organism. It has a wide range of biological activities in vitro as well as in vivo, such as anti-cancer, antioxidant, anti-inflammatory and beneficial cardiovascular effects. But, its low solubility in water led to its poor absorption in vivo and low bioavailability. Bovine serum album (BSA) nanoparticles have emerged as versatile desired carrier systems due to its ready availability, biodegradability, lack of toxicity and immunogenicity with fast development of nano technology. In this study, RES-BSANPS were prepared by a desolvation method and chemical cross-linking with glutaraldehyde successfully. Results controlled conditions (concentration of BSA, 10 mg/ml; pH = 9.0; volume of ethanol, 6 ml; volume of 0.25 % glutaraldehyde, 100 µl; amount of RES, 6.7 mg; cross-linking time, 24 h at room temperature (1 ml/min)) for entrapment efficiency, loading efficiency, mean particle size and zeta potential, were found to be 88.7 %, 39.4 %, 175.4 ± 0.5 nm, -35.93 ± 0.79 mV, respectively.
The Effects of Carnosine on High Glucose-Induced Apoptosis of Human Umbilical Vein Endothelial Cells
Authors: Shi Yan , Zhang Chun Jing
Published: September 2011

.Purposes,To explore the effects of carnosine on high glucose-induced apoptosis of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro. The cellular apoptotic model was made by the addition of high glucose (25 mmol/L), the group of high glucose and carnosine was administered by the addition of high glucose (25 mmol/L) with carnosine (20 mmol/L). In addition, cell apoptosis was detected by the electron microscopy and AnnexinV/PI flow cytometry. Results Compared with the control group, high glucose could induce HUVECs apoptosis under electron microscopy and AnnexinV/PI flow cytometry, while 20 mmol/L carnosine could inhibit the apoptosis induced by high glucose significantly (##P <0.05). Conclusion In this study, carnosine could inhibit high-glucose induced apoptosis of human umbilical vein endothelial cells.
The Study on Effects Resulted from Job Burnout on Performance Appraisal of Professional Coaches in China
Authors: Li Lin
Published: September 2011

Our study has conducted an inquiry ,based on the " The Structure scale for Job performance of Chinese professional coaches" and " Job burnout scale ", and 213 coaches from 19 provinces in China were involved in our investigation.The gathered evidence enable us to draw the conclusions that the two dimensions of low-potency feeling and Knowledge Drain result in significant negative impacts on task performance and development performance,low-potency feeling lead to negative impacts on contextual performance,on the contrary, the two dimensions of low-potency feeling and Knowledge Drain have shown significant positive effects on counter-production performance.
Analytical Hierarchy Process Model Applied to Port Urban Logistics Efficiency Commentary
Authors: Jiao Xin Long
Published: September 2011

This paper aims to construct analysis model of port urban logistics arrangement using Delphi and analytical hierarchy process method, furthermore, establishment of fuzzy theory and analytical hierarchy process model and factor set. And calculate every index weight with the weighting method--Gl based on differential principle and appraise comprehensive efficiency using fuzzy theory and analytical hierarchy process evaluation method. Using the average weighting method make quantitative disposal with indexes of appraisal factors and educes port urban logistics comprehensive efficiency outcome. And in so doing，validating Shanghai port urban logistics competitiveness actually which respects its efficiency status.
Effects of Annealing on Electrochemical Corrosion of Fe73.5Si13.5B9Nb3Cu1 Alloys
Authors: Li Ming Sheng , Tu Jin Wei , Zhang Shu Juan
Published: September 2011

The electrochemical corrosion affects soft-magnetic properties and service life of amorphous or nanocrystalline Fe73.5Si13.5B9Nb3Cu1 alloys. Therefore it is important to characterize the corrosion characteristic of the alloys for their potential application to engineering fields and further alloy development. In this study, the corrosion behavior of nanocrystalline Fe73.5Si13.5B9Nb3Cu1 alloys prepared by crystallization from the amorphous state was studied and compared with that of their amorphous counterparts by linear polarization (PLZ) and electrochemical impedance spectroscopy (EIS) technique. In electrolyte of 0.01 M NaCl, annealing at 550 °C leads to the decreased corrosion rate, while crystallization at 650 °C gives rise to the increase of corrosion rate. In the blend solution of 0.1 M NaCl and 0.1 M NaOH, heat-treatment at 550 °C or 650 °C improves the corrosion resistance for the alloys.
Effects of N-Doped Quantity and Current Density on Properties of TiO2 Films by Arc Ion Plating
Authors: Zhang Shu Juan , Li Ming Sheng , Chen Zhi Qin , Hu Jun Xi
Published: September 2011

TiO2thin films of different N-doped quantity and different current density were deposited on medical glass slide by arc ion plating. Film structure, surface morphology and optical properties were measured with XRD, SEM and UV-VIS transmittance spectroscope. Photo-catalytic performance of the films was evaluated by degradation methyl orange. The current density, O2partial pressure and N2partial pressure were varied in order to determine their effect on the properties of TiO2films. The study got the best doped process (current 50A，O250sccm, N2250 sccm, Ar 20sccm). In these experimental conditions, the absorption edge successfully moved to 510nm, the as-deposited TiO2films structure was composed with anatase and rutile. The annealing treatment made the crystal microscopy more obviously. The N-doped TiO2film not only reduced the UV catalytic performance , but also extended the catalytic properties to the sun light.
An Optimal Construction of a Hydropower Arch Gate
Authors: Cai Kun , Zhang Chao
Published: September 2011

A new design scheme of an emersed hydropower arch gate is presented based on topology optimization method. Three main components of the gate, i.e., two arms, a water-retaining face plate and its supporting frame, are considered in the present design method. Both of the layouts of the arms and the supporting frame are obtained by using topology optimization method instead of traditional experiments. In design process, firstly, the location, topology and shape of the arms of gate are obtained. Secondly, the layout of ribs in the support frame is found. Finally, the integrated structure is formed by using the components obtained above and the new gate satisfies such constraints as strength, stiffness and stability. The new gate is about 30% lighter than the one obtained by using traditional method, which means much material is saved and the new gate can be easier for operating.