bluuebunny/crossref_metadata_2025 huggingface dataset:
The Evolution of Life Modes in Stictidaceae, with Three Novel Taxa
Authors: Thiyagaraja Vinodhini , Lücking Robert , Ertz Damien , Karunarathna Samantha C. , Wanasinghe Dhanushka N. , Lumyong Saisamorn , Hyde Kevin D.
Published: February 2021

Ostropales sensu lato is a large group comprising both lichenized and non-lichenized fungi, with several lineages expressing optional lichenization where individuals of the same fungal species exhibit either saprotrophic or lichenized lifestyles depending on the substrate (bark or wood). Greatly variable phenotypic characteristics and large-scale phylogenies have led to frequent changes in the taxonomic circumscription of this order. Ostropales sensu lato is currently split into Graphidales, Gyalectales, Odontotrematales, Ostropales sensu stricto, and Thelenellales. Ostropales sensu stricto is now confined to the family Stictidaceae, which includes a large number of species that are poorly known, since they usually have small fruiting bodies that are rarely collected, and thus, their taxonomy remains partly unresolved. Here, we introduce a new genus Ostropomyces to accommodate a novel lineage related to Ostropa, which is composed of two new species, as well as a new species of Sphaeropezia, S. shangrilaensis. Maximum likelihood and Bayesian inference analyses of mitochondrial small subunit spacers (mtSSU), large subunit nuclear rDNA (LSU), and internal transcribed spacers (ITS) sequence data, together with phenotypic data documented by detailed morphological and anatomical analyses, support the taxonomic affinity of the new taxa in Stictidaceae. Ancestral character state analysis did not resolve the ancestral nutritional status of Stictidaceae with confidence using Bayes traits, but a saprotrophic ancestor was indicated as most likely in a Bayesian binary Markov Chain Monte Carlo sampling (MCMC) approach. Frequent switching in nutritional modes between lineages suggests that lifestyle transition played an important role in the evolution of this family.
In Vitro Characterization of Twenty-One Antifungal Combinations against Echinocandin-Resistant and -Susceptible Candida glabrata
Authors: Khalifa Hazim O. , Majima Hidetaka , Watanabe Akira , Kamei Katsuhiko
Published: February 2021

This study was designed to analyze the interaction of 21 antifungal combinations consisting of seven major antifungal agents against 11 echinocandin- susceptible and six-resistant C. glabrata isolates. The combinations were divided into five major groups and were evaluated by checkerboard, disc diffusion, and time-killing assays. Synergy based on the fractional inhibitory concentration index of ≤0.50 was observed in 17.65–29.41% of the cases for caspofungin combinations with azoles or amphotericin B. Amphotericin B combination with azoles induced synergistic interaction in a range of 11.76–29.41%. Azole combinations and 5-flucytosine combinations with azoles or amphotericin B did not show synergistic interactions. None of the 21 combinations showed antagonistic interactions. Interestingly, 90% of the detected synergism was among the echinocandin-resistant isolates. Disk diffusion assays showed that the inhibition zones produced by antifungal combinations were equal to or greater than those produced by single drugs. The time-killing assay showed the synergistic action of caspofungin combination with fluconazole, voriconazole, and posaconazole, and the amphotericin B-5-flucytosine combination. Furthermore, for the first time, this assay confirmed the fungicidal activity of caspofungin-voriconazole and amphotericin B-5-flucytosine combinations. The combination interactions ranged from synergism to indifference and, most importantly, no antagonism was reported and most of the synergistic action was among echinocandin-resistant isolates.
Exploring Risks in the Adoption of Business Intelligence in SMEs Using the TOE Framework
Authors: Stjepić Ana-Marija , Pejić Bach Mirjana , Bosilj Vukšić Vesna
Published: February 2021

The business success of small- and medium-sized enterprises (SMEs) increasingly relies on the adoption of various technological innovations. For today’s unpredictable business operations, business intelligence systems (BISs) represent one of the most prominent tools with a significant impact on business performance. However, different internal and external risks may influence BIS adoption. The goal of this paper is to investigate the risks that impact BIS adoption in SMEs, using the Technology, Organization, and Environment (TOE) framework. For that purpose, we develop the logistic regression model, using data collected by a questionnaire survey using a sample of 100 Croatian SMEs. The results indicate the applicability of the TOE theoretical framework for examining BIS adoption in SMEs. Given the results obtained, the sampled SMEs should take into account the internal risks related to the organizational dimension and external risks related to the environmental dimension. Our research did not reveal the significant impact of technological risks that encompass characteristics of considered technological innovation related to the technology dimension.
Banks’ Foreign Claims in the Aftermath of the 2008 Crisis: Institutional Response, Financial Efficiency, and Integration of Cross-Border Banking in the Euro Area
Authors: Warin Thierry , Stojkov Aleksandar
Published: February 2021

Beyond financial stability as the European Banking Union’s primary objective, the European capital market integration provides an impetus for deepening bank integration and greater financial market efficiency. This article proposes an empirical framework to assess the dynamics of euro area banks’ business networking. We use banks’ foreign claims across Europe, particularly the euro area, to see how banks react to various macroeconomic signals. Banks’ foreign claims are particularly interesting due to their sensitivity. One of the main conclusions is that the euro area has seen a reallocation of capital in the aftermath of the 2008 crisis. The financial picture of Europe is different after the recent financial crisis. Although we observe a re-concentration of capital from the periphery to the core countries, we also observe some signs of recovered confidence within the European banking framework for macro-prudential reasons.
The Relationship between Yield Curve and Economic Activity: An Analysis of G7 Countries
Authors: Kumar Ronald Ravinesh , Stauvermann Peter Josef , Vu Hang Thi Thu
Published: February 2021

The yield curve is an important tool to assess the economic progress of a country. In this study, we examine the strength of the relationship between term spread and economic activity, and between the components of the yield curve and economic activity in the G7 countries using monthly data on yield rates and seasonally adjusted data on the industrial production index (IPI). After matching the start and end date of the IPI with the yield rates, the data used and respective time period are as follows: Canada: March-1994 to December-2018, France: January-1999 to December-2018, Germany: October-2005 to December-2018, Italy: July-2009 to December-2018, Japan: July-1994 to January-2019, the UK: January-1994 to December-2018, and the US: February-1990 to January-2019. The results show positive associations between term spread and economic activity for Canada, France, Germany, Japan, the UK, and the US. For Italy, a negative association is noted. All three empirical factors could predict economic activity for France and Germany at the 12-month horizon only. For all other horizons, the factors’ ability to predict economic activity varies. We observe that by including additional macro-finance variables such as the current economic growth rate and the 3-month yield rate to capture the term structure level effects, the relationship between term spread and economic activity becomes stronger. This implies that the usefulness of yield curve and its decomposed components for the purpose of predicting economic activity should be cautiously modelled and employed for policy.
Effects of Agricultural Reclamation on Soil Physicochemical Properties in the Mid-Eastern Coastal Area of China
Authors: Xu Yan , Pu Lijie , Zhang Runsen , Zhu Ming , Zhang Meng , Bu Xinguo , Xie Xuefeng , Wang Yuan
Published: February 2021

Agricultural reclamation in coastal zones is effective for mitigating population pressure on the food supply. Soil properties are important factors influencing crop production in reclaimed coastal lands. This study aims to investigate the impacts of time and land use trajectories on soil physicochemical properties after reclamation. We sampled soils in areas that were reclaimed in 1999, 1998, 1991, 1989, 1986, 1981, and 1979 and determined some soil physicochemical properties such as electrical conductivity with a 1:5 soil:water ratio (EC1:5), exchange sodium percentage (ESP), sodium adsorption ratio (SAR), pH, organic matter (OM), total nitrogen (TN), alkaline hydrolyzable nitrogen (AN), cation exchange capacity (CEC), total phosphorus (TP), available phosphorus (TP) and soil particle size ratio. We analyzed their correlation with land use and the time since reclamation using one-way analysis of variance (ANOVA) and principal component analysis (PCA). The results showed that soil physicochemical properties changed significantly after agricultural reclamation. Soil EC1:5, ESP, and SAR declined rapidly, and OM, TN, and AN increased rapidly during the 29 years after reclamation. The soil particle size ratio was not significantly correlated with reclamation time. The land-use trajectories identified after reclamation had obvious effects on soil physicochemical properties. Aquaculture ponds were superior to cultivated land in terms of decreasing soil salinity but were inferior in terms of soil nutrient accumulation. In the future, more attention should be given to the environmental effects of agricultural reclaimed soils.
Building on “Traditional” Land Dispute Resolution Mechanisms in Rural Ghana: Adaptive or Anachronistic?
Authors: Asaaga Festus A.
Published: February 2021

Despite the ongoing land administration reforms being implemented across sub-Saharan Africa (SSA), including Ghana, as a viable pathway to achieve tenure security and greater efficiency in land administration, the subject of land dispute resolution has received relatively less attention. Whereas customary tenure institutions play a central role in land administration (controlling ~80% of all land in Ghana), they remain at the fringes of the formal land dispute adjudicatory process. Recognising the pivotal role of traditional institutions as development agents and potential vehicles for promoting good land governance, recent discourses on land tenure have geared toward mainstreaming traditional land dispute institutions into the architecture of the formal judicial process via alternative dispute resolution pathways. Yet, little is known, at least empirically, as to the operations of traditional dispute resolution institutions in the contemporary context. This study therefore explores the importance of traditional dispute resolution institutions in the management of land-related disputes in southcentral and western Ghana, drawing on data collated from 380 farming households operating 746 plots. The results show that contrary to the conventional thinking that traditional institutions are anachronistic and not fit for purpose, they remain strong and a preferred forum for land dispute resolution (proving resilient and adaptable), given the changing socio-economic and tenurial conditions. Yet, these forums have differing implications for different actors within the customary spheres accessing them. The results highlight practical ways for incorporating traditional dispute resolution in the overall land governance setup in Ghana and elsewhere in sub-Saharan Africa. This has implications for redesigning context-specific and appropriate land-use policy interventions that address local land dispute resolution.
Using Landscape Change Analysis and Stakeholder Perspective to Identify Driving Forces of Human–Wildlife Interactions
Authors: Mustățea Mihai , Pătru-Stupariu Ileana
Published: February 2021

Human–wildlife interactions (HWI) were frequent in the post-socialist period in the mountain range of Central European countries where forest habitats suffered transitions into built-up areas. Such is the case of the Upper Prahova Valley from Romania. In our study, we hypothesized that the increasing number of HWI after 1990 could be a potential consequence of woodland loss. The goal of our study was to analyse the effects of landscape changes on HWI. The study consists of the next steps: (i) applying 450 questionnaires to local stakeholders (both citizens and tourists) in order to collect data regarding HWI temporal occurrences and potential triggering factors; (ii) investigating the relation between the two variables through the Canonical Correspondence Analysis (CCA); (iii) modelling the landscape spatial changes between 1990 and 2018 for identifying areas with forest loss; (iv) overlapping the distribution of both the households affected by HWI and areas with loss of forested ecosystems. The local stakeholders indicate that the problematic species are the brown bear (Ursus arctos), the wild boar (Sus scrofa), the red fox (Vulpes vulpes) and the grey wolf (Canis lupus). The number of animal–human interactions recorded an upward trend between 1990 and 2018, and the most significant driving factors were the regulation of hunting practices, the loss of habitats, and artificial feeding. The landscape change analysis reveals that between 1990 and 2018, the forest habitats were replaced by built-up areas primarily on the outskirts of settlements, these areas coinciding with frequent HWI. The results are valid for both forest ecosystems conservation in the region, wildlife management, and human infrastructures durable spatial planning.
Transfer Phenomena in Bilingual Language Acquisition: The Case of Caused-Motion Constructions
Authors: Koch Nikolas , Günther Katharina
Published: February 2021

Usage-based approaches suggest that children gradually build abstract syntactic patterns, called constructions, through processes of abstraction and schematization from the input they receive. Bilingual children have the challenge of learning two sets of non-equivalent constructions when they build their constructicon. This can result in deviations from monolinguals, which are commonly referred to as transfer. Targeting the expression of the caused-motion construction, the present study focuses on idiosyncratic utterances, those that do not correspond to monolingual adult language use, in three different age groups (4, 6, and 8 years old) of German–French bilingual children in comparison to monolingual control groups. The quantitative analysis showed that idiosyncrasies could be found in both groups, but with significantly higher rates in bilinguals at all ages. In a qualitative analysis, idiosyncratic utterances were clustered into three different types: syntactic patterns, use of verbs, and directional phrases. Regarding the analysis of these types, the influence of French could be shown. In order to classify this linguistic phenomenon in a usage-based approach, we propose to consider transfer as a form of overgeneralization within the bilingual constructicon.
Synovial Joints. Tribology, Regeneration, Regenerative Rehabilitation and Arthroplasty
Authors: Popov Valentin L. , Poliakov Aleksandr M. , Pakhaliuk Vladimir I.
Published: February 2021

Synovial joints are unique biological tribosystems that allow a person to perform a wide range of movements with minimal energy consumption. In recent years, they have been increasingly called “smart friction units” due to their ability to self-repair and adapt to changing operating conditions. However, in reality, the elements of the internal structure of the joints under the influence of many factors can degrade rather quickly, leading to serious disease such as osteoarthritis. According to the World Health Organization, osteoarthritis is already one of the 10 most disabling diseases in developed countries. In this regard, at present, fundamental research on synovial joints remains highly relevant. Despite the fact that the synovial joints have already been studied fully, many issues related to their operating, prevention, development of pathology, diagnosis and treatment require more detailed consideration. In this article, we discuss the urgent problems that need to be solved for the development of new pharmacological agents, biomaterials, scaffolds, implants and rehabilitation devices for the prevention, rehabilitation and improvement of the treatment effectiveness of synovial joints at various stages of osteoarthritis.
Copper–Calcium Hydroxide and Permanent Electrophoretic Current for Treatment of Apical Periodontitis
Authors: Meto Agron , Droboniku Etleva , Blasi Elisabetta , Colombari Bruna , Tragaj Emiljano , Cervino Gabriele , Fiorillo Luca , Meto Aida
Published: February 2021

Endodontic failure has been and continues to be a problem for endodontics-specialists. Complicated anatomy, numerous foramens, and accessory canals are an environment for microorganisms to infect the teeth. The purpose of the present work was to evaluate the regeneration of copper–calcium hydroxide (Cupral)-endodontically treated teeth diagnosed with apical periodontitis using an electrophoresis technique. In total, 132 patients, aging from 19 to 65 years old, underwent endodontic treatment mono- and multi-radicular teeth, with complicated canals from January 2019 to June 2020. The patients were divided into two groups: (i) the control group—which included 54 patients (n = 62 teeth) receiving endodontic paste (Calcipast + 1) and, as final filling, the AH-PlusTM cement—and (ii) the Cupral group, which included 78 patients (n = 80 teeth) receiving Cupral paste plus the electrophoretic current and, as final filling, the Atacamit-alkaline cement. The clinical cases were periodically observed along an 18-month follow-up period via radiography. Data were expressed as focal size of the lesions (mean ± standard error (SEM) of all the radiographic outcomes) observed in each group at each interval point. Statistical analysis was performed using the Student’s t-test that allowed us to compare the control and Cupral groups; the statistical significance was set at p < 0.05 and p < 0.01, where the latter was highly significant. Before treatments, the focal sizes were 4.8 mm and 4.95 mm for control and Cupral-treated groups, respectively. After 6 months, the mean focal sizes were 3.9 mm and 2.14 mm for the control and Cupral groups, respectively. After 12 months, in the control group, the mean focal size was measured at 2.8 mm, while, in Cupral group, the lesion size decreased down to 0.31 mm and a highly dynamic regeneration of the destructive focal-bone occurred. After 18 months, the lesions were further significantly reduced in the control group (mean values of 2.62 mm), while they were barely detectable in the Cupral group (0.2 mm). In conclusion, we provide initial evidence that the Cupral-electrophoresis methodology is effective in treating destructive periodontitis of teeth with problematic canals up to 18 months, thus allowing teeth preservation.
Chemical Reaction and Ion Bombardment Effects of Plasma Radicals on Optoelectrical Properties of SnO2 Thin Films via Atomic Layer Deposition
Authors: Huang Pao-Hsun , Zhang Zhi-Xuan , Hsu Chia-Hsun , Wu Wan-Yu , Huang Chien-Jung , Lien Shui-Yang
Published: February 2021

In this study, the effect of radical intensity on the deposition mechanism, optical, and electrical properties of tin oxide (SnO2) thin films is investigated. The SnO2 thin films are prepared by plasma-enhanced atomic layer deposition with different plasma power from 1000 to 3000 W. The experimental results show that plasma contains different amount of argon radicals (Ar*) and oxygen radicals (O*) with the increased power. The three deposition mechanisms are indicated by the variation of Ar* and O* intensities evidenced by optical emission spectroscopy. The adequate intensities of Ar* and O* are obtained by the power of 1500 W, inducing the highest oxygen vacancies (OV) ratio, the narrowest band gap, and the densest film structure. The refractive index and optical loss increase with the plasma power, possibly owing to the increased film density. According to the Hall effect measurement results, the improved plasma power from 1000 to 1500 W enhances the carrier concentration due to the enlargement of OV ratio, while the plasma powers higher than 1500 W further cause the removal of OV and the significant bombardment from Ar*, leading to the increase of resistivity.
Phase Formation and Thermal Stability of Reactively Sputtered YTaO4–ZrO2 Coatings
Authors: Stelzer Bastian , Pingen Katrin , Hans Marcus , Holzapfel Damian M. , Richter Silvia , Mayer Joachim , Pradeep Konda Gokuldoss , Schneider Jochen M.
Published: February 2021

Y(1−x)/2Ta(1−x)/2ZrxO2 coatings with 0 to 44 mol% ZrO2 were synthesized by sputtering. Phase-pure M’-YTaO4 coatings were obtained at a substrate temperature of 900 °C. Alloying with ZrO2 resulted in the growth of M’ along with t-Zr(Y,Ta)O2 for ≤15 mol%, while for ≥28 mol%, ZrO2 X-ray diffraction (XRD) phase-pure metastable t was formed, which may be caused by small grain sizes and/or kinetic limitations. The former phase region transformed into M’ and M and the latter to an M’ + t and M + t phase region upon annealing to 1300 and 1650 °C, respectively. In addition to M and t, T-YTa(Zr)O4 phase fractions were observed at room temperature for ZrO2 contents ≥28 mol% after annealing to 1650 °C. T phase fractions increased during in situ heating XRD at 80 °C. At 1650 °C, a reaction with the α-Al2O3 substrate resulted in the formation of AlTaO4 and an Al-Ta-Y-O compound.
Next-Generation Materials for Energy Storage and Conversion
Authors: Kim Il Tae
Published: February 2021

n/a
Anti-Infective and Antiviral Activity of Valinomycin and Its Analogues from a Sea Cucumber-Associated Bacterium, Streptomyces sp. SV 21
Authors: Wibowo Joko T. , Kellermann Matthias Y. , Köck Matthias , Putra Masteria Y. , Murniasih Tutik , Mohr Kathrin I. , Wink Joachim , Praditya Dimas F. , Steinmann Eike , Schupp Peter J.
Published: February 2021

The manuscript investigated the isolation, characterization and anti-infective potential of valinomycin (3), streptodepsipeptide P11A (2), streptodepsipeptide P11B (1), and one novel valinomycin analogue, streptodepsipeptide SV21 (4), which were all produced by the Gram-positive strain Streptomycescavourensis SV 21. Although the exact molecular weight and major molecular fragments were recently reported for compound 4, its structure elucidation was not based on compound isolation and spectroscopic techniques. We successfully isolated and elucidated the structure based on the MS2 fragmentation pathways as well as 1H and 13C NMR spectra and found that the previously reported structure of compound 4 differs from our analysis. Our findings showed the importance of isolation and structure elucidation of bacterial compounds in the era of fast omics technologies. The here performed anti-infective assays showed moderate to potent activity against fungi, multi drug resistant (MDR) bacteria and infectivity of the Hepatitis C Virus (HCV). While compounds 2, 3 and 4 revealed potent antiviral activity, the observed minor cytotoxicity needs further investigation. Furthermore, the here performed anti-infective assays disclosed that the symmetry of the valinomycin molecule is most important for its bioactivity, a fact that has not been reported so far.
Polyhydroxy p-Terphenyls from a Mangrove Endophytic Fungus Aspergillus candidus LDJ-5
Authors: Zhou Guoliang , Zhang Xiaomin , Shah Mudassir , Che Qian , Zhang Guojian , Gu Qianqun , Zhu Tianjiao , Li Dehai
Published: February 2021

Six undescribed polyhydroxy p-terphenyls, namely asperterphenyllins A–F, were isolated from an endophytic fungus Aspergillus candidus LDJ-5. Their structures were determined by NMR and MS data. Differing from the previously reported p-terphenyls, asperterphenyllin A represents the first p-terphenyl dimer connected by a C-C bond. Asperterphenyllin A displayed anti-influenza virus A (H1N1) activity and protein tyrosine phosphatase 1B (PTP1B) inhibitory activity with IC50 values of 53 μM and 21 μM, respectively. The anti-influenza virus A (H1N1) activity and protein tyrosine phosphatase 1B (PTP1B) inhibitory activity of p-terphenyls are reported for the first time. Asperterphenyllin G exhibited cytotoxicity against nine cell lines with IC50 values ranging from 0.4 to 1.7 μM. Asperterphenyllin C showed antimicrobial activity against Proteus species with a MIC value of 19 μg/mL.
Performance Analysis of Electrochemical Micro Machining of Titanium (Ti-6Al-4V) Alloy under Different Electrolytes Concentrations
Authors: Thangamani Geethapriyan , Thangaraj Muthuramalingam , Moiduddin Khaja , Mian Syed Hammad , Alkhalefah Hisham , Umer Usama
Published: February 2021

Titanium alloy is widely used in modern automobile industries due to its higher strength with corrosion resistance. Such higher strength materials can be effectively machined using unconventional machining processes, especially the electro-chemical micro machining (ECMM) process. It is important to enhance the machining process by investigating the effects of electrolytes and process parameters in ECMM. The presented work describes the influence of three different combinations of Sodium Chloride-based electrolytes on machining Titanium (Ti-6Al-4V) alloy. Based on the ECMM process parameters such as applied voltage, electrolytic concentration, frequency and duty cycle on response, characteristics are determined by the Taguchi design of experiments. The highest material removal rate (MRR) was achieved by the Sodium Chloride and Sodium Nitrate electrolyte. The combination of Sodium Chloride and Citric Acid achieve highest Overcut and Circularity. The optimal overcut was observed from the Sodium Chloride and Glycerol electrolyte due to the presence of glycerol. The better conicity was obtained from Sodium Chloride and Citric Acid in comparison with other electrolytes. A Sodium Chloride and Glycerol combination could generate better machined surface owing to the chelating effect of Glycerol.
Enhanced Cementation of Co2+ and Ni2+ from Sulfate and Chloride Solutions Using Aluminum as an Electron Donor and Conductive Particles as an Electron Pathway
Authors: Choi Sanghyeon , Jeon Sanghee , Park Ilhwan , Ito Mayumi , Hiroyoshi Naoki
Published: February 2021

Cobalt and nickel have become important strategic resources because they are widely used for renewable energy technologies and rechargeable battery production. Cementation, an electrochemical deposition of noble metal ions using a less noble metal as an electron donor, is an important option to recover Co and Ni from dilute aqueous solutions of these metal ions. In this study, cementation experiments for recovering Co2+ and Ni2+ from sulfate and chloride solutions (pH = 4) were conducted at 298 K using Al powder as electron donor, and the effects of additives such as activated carbon (AC), TiO2, and SiO2 powders on the cementation efficiency were investigated. Without additives, cementation efficiencies of Co2+ and Ni2+ were almost zero in both sulfate and chloride solutions, mainly because of the presence of an aluminum oxide layer (Al2O3) on an Al surface, which inhibits electron transfer from Al to the metal ions. Addition of nonconductor (SiO2) did not affect the cementation efficiencies of Co2+ and Ni2+ using Al as electron donor, while addition of (semi)conductors such as AC or TiO2 enhanced the cementation efficiencies significantly. The results of surface analysis (Auger electron spectroscopy) for the cementation products when using TiO2/Al mixture showed that Co and Ni were deposited on TiO2 particles attached on the Al surface. This result suggests that conductors such as TiO2 act as an electron pathway from Al to Co2+ and Ni2+, even when an Al oxide layer covered on an Al surface.
Flotation of Seafloor Massive Sulfide Ores: Combination of Surface Cleaning and Deactivation of Lead-Activated Sphalerite to Improve the Separation Efficiency of Chalcopyrite and Sphalerite
Authors: Aikawa Kosei , Ito Mayumi , Kusano Atsuhiro , Park Ilhwan , Oki Tatsuya , Takahashi Tatsuru , Furuya Hisatoshi , Hiroyoshi Naoki
Published: February 2021

The purpose of this study is to propose the flotation procedure of seafloor massive sulfide (SMS) ores to separate chalcopyrite and galena as froth and sphalerite, pyrite, and other gangue minerals as tailings, which is currently facing difficulties due to the presence of water-soluble compounds. The obtained SMS ore sample contains CuFeS2, ZnS, FeS2, SiO2, and BaSO4 in addition to PbS and PbSO4 as Pb minerals. Soluble compounds releasing Pb, Zn2+, Pb2+, and Fe2+/3+ are also contained. When anglesite co-exists, lead activation of sphalerite occurred, and thus sphalerite was recovered together with chalcopyrite as froth. To remove soluble compounds (e.g., anglesite) that have detrimental effects on the separation efficiency of chalcopyrite and sphalerite, surface cleaning pretreatment using ethylene diamine tetra acetic acid (EDTA) was applied before flotation. Although most of anglesite were removed and the recovery of chalcopyrite was improved from 19% to 81% at 20 g/t potassium amyl xanthate (KAX) after EDTA washing, the floatability of sphalerite was not suppressed. When zinc sulfate was used as a depressant for sphalerite after EDTA washing, the separation efficiency of chalcopyrite and sphalerite was improved due to deactivation of lead-activated sphalerite by zinc sulfate. The proposed flotation procedure of SMS ores—a combination of surface cleaning with EDTA to remove anglesite and the depression of lead-activated sphalerite by using zinc sulfate—could achieve the highest separation efficiency of chalcopyrite and sphalerite; that is, at 200 g/t KAX, the recoveries of chalcopyrite and sphalerite were 86% and 17%, respectively.
Comparative Study of Eleven Mechanical Pretreatment Protocols for Cryptosporidium parvum DNA Extraction from Stool Samples
Authors: Claudel Laure , Valeix Nicolas , Basmaciyan Louise , Pereira Bruno , Costa Damien , Vincent Anne , Valot Stéphane , Favennec Loic , Dalle Frederic
Published: February 2021

Nowadays, many commercial kits allow the polymerase chain reaction (PCR) detection of Cryptosporidium deoxyribonucleic acid (DNA) in stool samples, the efficiency of which relies on the extraction method used. Mechanical pretreatment of the stools using grinding beads has been reported to greatly improve this extraction step. However, optimization of this key step remains to be carried out. Indeed, many parameters could influence the pretreatment performances, among which the modulation of the speed and duration of the grinding step, in addition to the physicochemical features of the grinding beads, have never been evaluated to date. In this study, eleven commercial mechanical pretreatment matrixes (Lysis matrix tubes®, MP Biomedical, Irvine, CA, USA) composed of beads with different sizes, shapes, and molecular compositions, were evaluated for their performances in improving Cryptosporidium parvum oocyst DNA extraction before amplification by using our routinely used real-time PCR method. As expected, the eleven commercial mechanical pretreatment matrixes showed varying performances depending on the composition, size, and shape. All in all, the best performances were obtained when using the Lysing matrix, including ceramic beads with a median size (diameter of 1.4 mm).
Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration
Authors: Pedro Nicole , Silva Cláudio N. , Magalhães Ana C. , Cavadas Bruno , Rocha Ana M. , Moreira Ana C. , Gomes Maria Salomé , Silva Diogo , Sobrinho-Simões Joana , Ramos Angélica , Cardoso Maria J. , Filipe Rita , Palma Pedro , Ceia Filipa , Silva Susana , Guimarães João T. , Sarmento António , Fernandes Verónica , Pereira Luisa , Tavares Margarida
Published: February 2021

A few molecularly proven severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases of symptomatic reinfection are currently known worldwide, with a resolved first infection followed by a second infection after a 48 to 142-day intervening period. We report a multiple-component study of a clinically severe and prolonged viral shedding coronavirus disease 2019 (COVID-19) case in a 17-year-old Portuguese female. She had two hospitalizations, a total of 19 RT-PCR tests, mostly positive, and criteria for releasing from home isolation at the end of 97 days. The viral genome was sequenced in seven serial samples and in the diagnostic sample from her infected mother. A human genome-wide array (>900 K) was screened on the seven samples, and in vitro culture was conducted on isolates from three late samples. The patient had co-infection by two SARS-CoV-2 lineages, which were affiliated in distinct clades and diverging by six variants. The 20A lineage was absolute at the diagnosis (shared with the patient’s mother), but nine days later, the 20B lineage had 3% frequency, and two months later, the 20B lineage had 100% frequency. The 900 K profiles confirmed the identity of the patient in the serial samples, and they allowed us to infer that she had polygenic risk scores for hospitalization and severe respiratory disease within the normal distributions for a Portuguese population cohort. The early-on dynamic co-infection may have contributed to the severity of COVID-19 in this otherwise healthy young patient, and to her prolonged SARS-CoV-2 shedding profile.
Virulence Properties of mcr-1-Positive Escherichia coli Isolated from Retail Poultry Meat
Authors: Kubelová Michaela , Koláčková Ivana , Gelbíčová Tereza , Florianová Martina , Kalová Alžběta , Karpíšková Renáta
Published: February 2021

The great plasticity and diversity of the Escherichia coli genome, together with the ubiquitous occurrence, make E. coli a bacterium of world-wide concern. Of particular interest are pathogenic strains and strains harboring antimicrobial resistance genes. Overlapping virulence-associated traits between avian-source E. coli and human extraintestinal pathogenic E. coli (ExPEC) suggest zoonotic potential and safety threat of poultry food products. We analyzed whole-genome sequencing (WGS) data of 46 mcr-1-positive E. coli strains isolated from retail raw meat purchased in the Czech Republic. The investigated strains were characterized by their phylogroup—B1 (43%), A (30%), D (11%), E (7%), F (4%), B2 (2%), C (2%), MLST type, and serotype. A total of 30 multilocus sequence types (STs), of which ST744 was the most common (11%), were identified, with O8 and O89 as the most prevalent serogroups. Using the VirulenceFinder tool, 3 to 26 virulence genes were detected in the examined strains and a total of 7 (15%) strains met the pathogenic criteria for ExPEC. Four strains were defined as UPEC (9%) and 18 (39%) E. coli strains could be classified as APEC. The WGS methods and available on-line tools for their evaluation enable a comprehensive approach to the diagnosis of virulent properties of E. coli strains and represent a suitable and comfortable platform for their detection. Our results show that poultry meat may serve as an important reservoir of strains carrying both virulence and antibiotic resistance genes for animal and human populations.
Improved Water–Gas Shift Performance of Au/NiAl LDHs Nanostructured Catalysts via CeO2 Addition
Authors: Gabrovska Margarita , Ivanov Ivan , Nikolova Dimitrinka , Krstić Jugoslav , Venezia Anna Maria , Crişan Dorel , Crişan Maria , Tenchev Krassimir , Idakiev Vasko , Tabakova Tatyana
Published: February 2021

Supported gold on co-precipitated nanosized NiAl layered double hydroxides (LDHs) was studied as an effective catalyst for medium-temperature water–gas shift (WGS) reaction, an industrial catalytic process traditionally applied for the reduction in the amount of CO in the synthesis gas and production of pure hydrogen. The motivation of the present study was to improve the performance of the Au/NiAl catalyst via modification by CeO2. An innovative approach for the direct deposition of ceria (1, 3 or 5 wt.%) on NiAl-LDH, based on the precipitation of Ce3+ ions with 1M NaOH, was developed. The proposed method allows us to obtain the CeO2 phase and to preserve the NiAl layered structure by avoiding the calcination treatment. The synthesis of Au-containing samples was performed through the deposition–precipitation method. The as-prepared and WGS-tested samples were characterized by X-ray powder diffraction, N2-physisorption and X-ray photoelectron spectroscopy in order to clarify the effects of Au and CeO2 loading on the structure, phase composition, textural and electronic properties and activity of the catalysts. The reduction behavior of the studied samples was evaluated by temperature-programmed reduction. The WGS performance of Au/NiAl catalysts was significantly affected by the addition of CeO2. A favorable role of ceria was revealed by comparison of CO conversion degree at 220 °C reached by 3 wt.% CeO2-modified and ceria-free Au/NiAl samples (98.8 and 83.4%, respectively). It can be stated that tuning the properties of Au/NiAl LDH via CeO2 addition offers catalysts with possibilities for practical application owing to innovative synthesis and improved WGS performance.
Sonochemical Synthesis, Characterization and Optical Properties of Tb-Doped CdSe Nanoparticles: Synergistic Effect between Photocatalysis and Sonocatalysis
Authors: Hanifehpour Younes , Nozad Ashan Narges , Amani-Ghadim Ali , Joo Sang
Published: February 2021

In this study, Tb-doped CdSe nanoparticles with variable Tb3+ content were synthesized by a simple sonochemical technique. The synthesized nanoparticles were characterized by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and powder X-ray diffraction (XRD). The sono-photocatalytic activities of the as-prepared specimens were assessed for the degradation of Reactive Black 5. The experimental results show that the sono-photocatalytic process (85.25%) produced a higher degradation percentage than the individual sono- (22%) and photocatalytic degradation (8%) processes for an initial dye concentration and Tb-doped CdSe dosage of 20 mg/L and 1 g/L, respectively. Response surface methodology (RSM) was utilized to assess model and optimize the impacts of the operational parameters, namely, the Tb3+ content, initial dye concentration, catalyst dosage, and time. The addition of benzoquinone results in remarkably inhibited degradation and the addition of ammonium oxalate reduced the removal percentage to 24%. Superoxide radicals and photogenerated holes were detected as the main oxidative species.
Protein Substitutes in PKU; Their Historical Evolution
Authors: Daly Anne , Evans Sharon , Pinto Alex , Ashmore Catherine , MacDonald Anita
Published: February 2021

Protein substitutes developed for phenylketonuria (PKU) are a synthetic source of protein commonly based on L-amino acids. They are essential in the treatment of phenylketonuria (PKU) and other amino acid disorders, allowing the antagonistic amino acid to be removed but with the safe provision of all other amino acids necessary for maintaining normal physiological function. They were first formulated by a chemist and used experimentally on a 2-year-old girl with PKU and their nutritional formulations and design have improved over time. Since 2008, a bioactive macropeptide has been used as a base for protein substitutes in PKU, with potential benefits of improved bone and gut health, nitrogen retention, and blood phenylalanine control. In 2018, animal studies showed that physiomimic technology coating the amino acids with a polymer allows a slow release of amino acids with an improved physiological profile. History has shown that in PKU, the protein substitute’s efficacy is determined by its nutritional profile, amino acid composition, dose, timing, distribution, and an adequate energy intake. Protein substitutes are often given little importance, yet their pharmacological actions and clinical benefit are pivotal when managing PKU.
Back in Time for Breakfast: An Analysis of the Changing Breakfast Cereal Aisle
Authors: Croisier Emilie , Hughes Jaimee , Duncombe Stephanie , Grafenauer Sara
Published: February 2021

Breakfast cereal improves overall diet quality yet is under constant scrutiny with assertions that the category has not improved over time. This study aimed to comprehensively analyse the category of breakfast cereals, the nutritional values, and health claims across eight distinct sub-categories at four time points (2013, 2015, 2018, and 2020). An audit of products from four major supermarkets in metropolitan Sydney (Aldi, Coles, IGA, and Woolworths) collected ingredient lists, nutrition information, claims and Health Star Rating (HSR) for biscuits and bites; brans; bubbles, puffs, and flakes; granola and clusters; hot cereal flavoured; hot cereal plain; muesli; breakfast biscuits. The median (IQR) were calculated for energy, protein, fat, saturated fat, carbohydrate, sugars, dietary fibre, and sodium for comparisons over time points by nutrient. Data from 2013 was compared with 2020 (by sub-category and then for a sub-section of common products available at each time point). Product numbers between 2013 (n = 283) and 2020 (n = 543) almost doubled, led by granola and clusters. Whole grain cereals ≥ 8 g/serve made up 67% of products (↑114%). While there were positive changes in nutrient composition over time within the full data set, the most notable changes were in the nutrition composition of cereals marketed as the same product in both years (n = 134); with decreases in mean carbohydrate (2%), sugar (10%) and sodium (16%) (p < 0.000), while protein and total fat increased significantly (p = 0.036; p = 0.021). Claims regarding Dietary Fibre and Whole Grain doubled since 2013. Analysis of sub-categories of breakfast cereal assisted in identifying some changes over time, but products common to both timeframes provided a clearer analysis of change within the breakfast category, following introduction of HSR. Whole grain products were lower in the two target nutrients, sodium and sugars, and well-chosen products represent a better choice within this category.
Eugenol-Containing Essential Oils Loaded onto Chitosan/Polyvinyl Alcohol Blended Films and Their Ability to Eradicate Staphylococcus aureus or Pseudomonas aeruginosa from Infected Microenvironments
Authors: Antunes Joana C. , Tavares Tânia D. , Teixeira Marta A. , Teixeira Marta O. , Homem Natália C. , Amorim M. Teresa P. , Felgueiras Helena P.
Published: February 2021

Chronic wounds (CW) create numerous entryways for pathogen invasion and prosperity, further damaging host tissue and hindering its remodeling and repair. Essential oils (EOs) exert quick and efficient antimicrobial (AM) action, unlikely to induce bacterial resistance. Cinnamon leaf and clove oils (CLO and CO) display strong AM activity, namely against Staphylococcus aureus and Pseudomonas aeruginosa. Chitosan (CS) is a natural and biodegradable cationic polysaccharide, also widely known for its AM features. CS and poly (vinyl alcohol) (PVA) films were prepared (ratio 30/70 w/w; 9 wt%) by the solvent casting and phase inversion method. The film’s thermal stability and chemical composition data reinforced polymer blending and EO entrapment. Films were supplemented with 1 and 10 wt% of EO in relation to total polymeric mass. The film thickness and degree of swelling (DS) tended to increase with EO content, particularly with 10 wt % CLO (* p < 0.05). UV-visible absorbance scans in the 250–320 cm−1 region confirmed the successful uptake of CLO and CO into CS/PVA films, particularly with films loaded with 10 wt% EO that contained 5.30/5.32 times more CLO/CO than films supplemented with 1 wt% EO. AM testing revealed that CS films alone were effective against both bacteria and capable of eradicating all P. aeruginosa within the hour (*** p < 0.001). Still, loaded CS/PVA films showed significantly improved AM traits in relation to unloaded films within 2 h of contact. This study is a first proof of concept that CLO and CO can be dispersed into CS/PVA films and show bactericidal effects, particularly against S. aureus, this way paving the way for efficient CW therapeutics.
Optical Chemical Sensor Based on 2,2-Furildioxime in Sol-Gel Matrix for Determination of Ni2+ in Water
Authors: Suherman Suherman , Hakim Muh. Supwatul , Kuncaka Agus
Published: February 2021

A new optical chemical sensor was fabricated based on incorporation of 2,2-furildioxime as a sensitive reagent into the nanopore of a transparent glasslike material through the sol-gel method which was suitable for determination of Ni2+ ions in aqueous solutions. The prepared sensors were composed of tetraethoxysilane (TEOS), 2,2-furildioxime, methanol, hydrochloric acid and Triton X-100. The sensors were constructed by dip coating onto glass substrates. The optimum response of the sensor toward Ni2+ ions was reached at pH 8.5 and the contact time for the formation of the complex at 10 min. The linear concentration of the calibration curve was in the range of 1–5 mg L−1 with a detection limit of 0.111 mg L−1, and quantification limit of 0.337 mg L−1. In addition, the relative standard deviation (RSD) was less than 5% in determination of Ni2+ with ten slide sensor membranes. The developed sensor was tested on Ni2+ determination in real water samples which was confirmed by the atomic absorption spectrophotometer method.
Coupling Microbial Electrolysis Cell and Activated Carbon Biofilter for Source-Separated Greywater Treatment
Authors: Dhadwal Mayank , Liu Yang , Dhar Bipro Ranjan
Published: February 2021

Reclamation and reuse of wastewater are increasingly viewed as a pragmatic tool for water conservation. Greywater, which includes water from baths, washing machines, dishwashers, and kitchen sinks, is a dilute wastewater stream, making it an attractive stream for extraction of non-potable water. However, most previous studies primarily focused on passively aerated biological and physicochemical treatment processes for greywater treatment. Here, we investigated an integrated process of a microbial electrochemical cell (MEC) followed by granular activated carbon (GAC) biofilter for greywater treatment. The integrated system could achieve 99.3% removal of total chemical oxygen demand (TCOD) and 98.7% removal of the anionic surfactants (linear alkylbenzene sulphonates) from synthetic greywater at a total hydraulic residence time (HRT) of 25 h (1 day for MEC and 1 h for GAC biofilter). For one-day HRT, the maximum peak volumetric current density from MEC was 0.65 A/m3, which was comparable to that achieved at four-day HRT (0.66 A/m3). The adsorption by GAC was identified as a key mechanism for the removal of organics and surfactants. In addition, recirculation of liquid within the GAC biofilter was identified as a critical factor in achieving high-rate treatment. Although results indicated that GAC biofilter could be a standalone process for greywater, MEC can provide an opportunity for potential energy recovery from greywater. However, further studies should focus on developing high-rate MECs with higher energy recovery potential for practical operation.