bluuebunny/crossref_metadata_2025 huggingface dataset:
Valorization of Persimmon Fruit Through the Development of New Food Products
Authors: Hosseininejad Sepideh , González Cristina M. , Hernando Isabel , Moraga Gemma
Published: June 2022

Persimmon (Diospyros kaki Thunb.) fruits are among the most widely cultivated fruit crops worldwide, they are widespread in Asian countries and Europe, and their production is increasing along with the demand of consumers. Persimmon is a good source of nutrients and bioactive compounds, especially dietary fiber, carotenoids, and phenolic compounds, among other bioactive phytochemicals. However, persimmon is among the fruits, with a significant postharvest loss over the last few years. The cultivation of persimmon has a limited shelf life; it is a seasonal fruit and is perishable and difficult to store and transport; therefore, many persimmons are discarded. Currently, there is an increase in the valorization of the discarded persimmon fruits to generate opportunities and create a more sustainable system, in line with a possible circular economy. Therefore, in this review, we aim to compile an updated and brief revision of persimmons’ main chemical and bioactive compounds and the potential use of persimmon surpluses and by-products in developing new food products.
Research, Teaching, and Knowledge Transfer Assessment in Spain: Strategies and Results
Authors: Docampo Domingo , Safón Vicente , Albert Carlos
Published: June 2022

In Spain, faculty members' and researchers' activities are assessed for three main reasons: applying for university teacher and researcher positions, developing their academic careers, and calculating their individual remunerations. This assessment covers three dimensions of university activity: teaching, research, and knowledge transfer. Over the last 20 years, the level of career-related requirements has been raised considerably with the introduction of a habilitation system in 2001, which was followed by a new accreditation system in 2007, both national in scope and beyond the control of individual universities. On the other hand, during the last three decades, three distinct financial incentives have been introduced for tenure-track positions: one for teaching achievements, which was introduced in 1989, is awarded every five years, and is managed by the universities; a research incentive that was created in 1989, is available every 6 years, and is managed outside the universities; and another one that has been recently created to reward knowledge-transfer achievements. In this article, we analyze the results achieved through these instruments and conclude that the incentive schemes taken out of the academic control of university governance were the most successful ones.
Distributive Justice and Urban Form Adaptation to Flooding Risks: Spatial Analysis to Identify Toronto's Priority Neighborhoods
Authors: Mohtat Niloofar , Khirfan Luna
Published: June 2022

Empirical evidence points out that urban form adaptation to climate-induced flooding events—through interventions in land uses and town plans (i. e., street networks, building footprints, and urban blocks)—might exacerbate vulnerabilities and exposures, engendering risk inequalities and climate injustice. We develop a multicriteria model that draws on distributive justice's interconnections with the risk drivers of social vulnerabilities, flood hazard exposures, and the adaptive capacity of urban form (through land uses and town plans). The model assesses “who” is unequally at-risk to flooding events, hence, should be prioritized in adaptation responses; “where” are the high-risk priority areas located; and “how” can urban form adaptive interventions advance climate justice in the priority areas. We test the model in Toronto, Ontario, Canada, where there are indications of increased rainfall events and disparities in social vulnerabilities. Our methodology started with surveying Toronto-based flooding experts who assigned weights to the risk drivers based on their importance. Using ArcGIS, we then mapped and overlayed the risk drivers' values in all the neighborhoods across the city based on the experts' assigned weights. Accordingly, we identified four high-risk tower communities with old infrastructure and vulnerable populations as the priority neighborhoods for adaptation interventions within the urban form. These four neighborhoods are typical of inner-city tower blocks built in the 20th century across North America, Europe, and Asia based on modern architectural ideas. Considering the lifespan of these blocks, this study calls for future studies to investigate how these types of neighborhoods can be adapted to climate change to advance climate justice.
Autonomous Crosslink Radionavigation for a Lunar CubeSat Mission
Authors: Turan Erdem , Speretta Stefano , Gill Eberhard
Published: June 2022

This study presents an autonomous orbit determination system based on crosslink radiometric measurements applied to a future lunar CubeSat mission to clearly highlight its advantages with respect to existing ground-based navigation strategies. This work is based on the Linked Autonomous Interplanetary Satellite Orbit Navigation (LiAISON) method which provides an autonomous navigation solution solely using satellite-to-satellite measurements, such as range and/or range-rate, to estimate absolute spacecraft states when at least one of the involved spacecraft has an orbit with a unique size, shape, and orientation. The lunar vicinity is a perfect candidate for this type of application due to the asymmetrical gravity field: the selected lunar mission, an Earth-Moon L2(EML2) Halo orbiter, has an inter-satellite link between a lunar elliptical frozen orbiter. Simulation results show that, even in case of high-measurement errors (in the order of 100 m, 1σ), the navigation filter estimates the true states of spacecraft at EML2 with an error in the order of 500 m for position, and 2 mm/s for velocity, respectively and the elliptical lunar frozen orbiter states can be estimated in the order of 100 m for position and 1 cm/s for velocity, respectively. This study shows that range-only measurements provide better state estimation than range-rate-only measurements for this specific situation. Different bias handling strategies are also investigated. It has been found that even a less accurate ranging method, such as data-aided ranging, provides a sufficient orbit determination solution. This would simplify the communication system design for the selected CubeSat mission. The most observable states are found to be position states of the lunar orbiter via the observability analysis. In addition, the best tracking windows are also investigated for the selected mission scenario.
Microplastics and Brominated Flame Retardants in Freshwater Fishes From Italian Lakes: Implication for Human Health
Authors: Pittura Lucia , Tavoloni Tamara , Ventura Lucia , Stramenga Arianna , d'Errico Giuseppe , Lo Vaglio Giovanni , Regoli Francesco , Piersanti Arianna , Gorbi Stefania
Published: June 2022

Microplastics (MPs) and brominated flame retardants (BFRs) represent a synergic threat for aquatic environments and organisms' health status, with an additional concern over food quality and food security for species of commercial interest. In this study, the ingestion of MPs, levels of polybrominated diphenyl ethers (PBDEs), and hexabromocyclododecane (HBCDs) were assessed in organisms from two lakes of Central Italy, with the aims of exploring the bioavailability of these pollutants in freshwater environments, the possible translocation of MPs from digestive to edible tissues, and the relationship between MPs ingestion and bioaccumulation of BFRs. The fish Perca fluviatilis, Anguilla anguilla, Carassus auratus, and the crayfish Procambarus clarkii, all species commercialized for human consumption, were caught in Trasimeno Lake; moreover, P. fluviatilis and Rutilus rutilus were also sampled in Piediluco, a lake strongly influenced by industrial and anthropogenic activities, where fishing for commercial purpose is forbidden. With the exception of C. auratus which showed the highest frequency of MPs ingestion (75%), species from Piediluco Lake exhibited a more elevated percentage of organisms positive to MPs ingestion (45%) and higher levels of PBDEs and HBCDs (mean values of 343 and 792 pg/g, respectively, in P. fluviatilis; 445 and 677 pg/g, respectively, in R. rutilus) than Trasimeno species (25% frequency of MPs ingestion, mean values between 6 and 163 pg/g for PBDEs and 5-107 pg/g for HBCDs). Polyester fibers dominated among MP typologies, and a high occurrence of man-made natural fibers was recorded. The number of MPs extracted in the gastrointestinal tracts of fish and soft bodies of crayfishes positive to MPs ingestion ranged between 1 and 2, whereas no MPs were found in fish filets. Given these results, the risk related to human consumption of Trasimeno organisms appears very low, whereas further investigations are required to better elucidate the possible role of MPs pollution in modulating chemical bioaccumulation in edible tissues. This study contributed to assess both environmental quality and food safety, reinforcing the use of bioindicator species for monitoring plans, in accordance with European recommendations.
Characterization of Unripe Grapes (Vitis vinifera L.) and Its Use to Obtain Antioxidant Phenolic Compounds by Green Extraction
Authors: Bayram Yuksel , Elgin Karabacak Cigdem
Published: June 2022

Thinning pruning is a process in modern viticulture to improve product quality when grapes are still in the immature fruit stage. Unripe grapes, which are waste, are mostly consumed locally as verjuices to meet domestic demand. This study aims to optimize a more efficient “green” technique for the extraction of total phenolic compounds (TPC) and antioxidant capacity (AC) from three varieties of unripe grape juice by response surface method (RSM) using ultrasound-assisted extraction (UAE). The influence of temperature (25–65°C) and extraction time (5–30 min) on total phenolic content (TPC) and antioxidant capacity (DPPH and CUPRAC) was investigated. In this study, physicochemical properties, mineral composition, phenolic and organic acid components of three different verjuices were also investigated. As a result, the optimal extraction points for extraction time (12 min) and temperature (30°C) were determined. All independent variables were found to be significantly effective on TPC and AC content during UAE. Verjuices are a rich source of antioxidants, phenols, organic acids, minerals, and vitamin C. This study is expected to contribute to the evaluation of unripe grape wastes, which are very rich in bioactive components, and to increase its economic potential by expanding local production, thus contributing to sustainable agri-food processing.
Prognostic Nutritional Index in Hepatocellular Carcinoma Patients With Hepatitis B Following US-Guided Percutaneous Microwave Ablation: A Retrospective Study With 1,047 Patients
Authors: Wang Yaxi , Li Xin , Yu Jie , Cheng ZhiGang , Hou Qidi , Liang Ping
Published: June 2022

Objective

Several studies have revealed that the prognostic nutritional index (PNI) was associated with survival in several cancers. However, the prognostic value of PNI in hepatocellular carcinoma (HCC) patients following ultrasound-guided percutaneous microwave ablation (US-PMWA) remains unknown, especially in patients with hepatitis B virus (HBV) infection. Therefore, the present study aimed to evaluate the potential prognostic value of PNI in these patients.

Materials

The medical records of 1,047 HCC patients with HBV infection following US-PMWA were retrospectively reviewed. The association between preoperative PNI and overall survival (OS), as well as other clinical characteristics of HCC, were analyzed using the Kaplan–Meier plot, log-rank test, multi-parameter Cox proportional hazards model, restricted cubic spline (RCS), and time-dependent receiver operating characteristic (ROC) curve analyses.

Results

Patients with a preoperative PNI more than 45 were verified to have better OS than patients with a PNI less than 45. In the multi-parameter Cox proportional hazards models, the log-transformed PNI was verified as an independent prognostic factor for OS. The result of the RCS analysis revealed that there was a nearly linear relationship between PNI and OS. The area under the time-dependent ROC curve for PNI in predicting OS was 0.56, which is relatively stable.

Conclusion

Preoperative PNI represents a convenient, noninvasive, and independent prognostic indicator in HCC patients with HBV infection following US-PMWA.
Improving Cooling Capacity of Condensation-Free Radiant Cooling for Low-Emissivity Chilled Ceiling via Adaptive Double-Skin Infrared Membranes
Authors: Du Ke , Wu Huijun , Guo Yanling , Huang Gongsheng , Xu Xinhua , Liu Yanchen
Published: June 2022

Radiant cooling has well been acknowledged as energy efficient and thermal comfortable technology compared to conventional convective cooling. However, the radiant cooling exists two serious problems (viz., insufficient cooling capacity and high condensation risk) especially in hot and humid climate zones. By adding double-skin infrared transparent membranes (DIMs) onto radiant cooling panel, the air-contact surface can be separated from the cooling source surface, which makes it possible to use a low-temperature cooling source while maintaining air-contact surface higher than dew point temperature. The DIMs are transparent to radiant heat transfer which yields great cooling capacity while chilled ceiling has high emissivity (e.g., above 0.9). However, for metal chilled ceilings having low emissivity, radiant heat from cooling load to chilled ceiling would be reduced through DIMs, which results in insufficient cooling capacity. In this paper, a type of adaptive double-skin infrared membranes (a-DIMs) consisting a high-emissivity membrane and a high transparent membrane is proposed to improve cooling capacity of conventional metal chilled ceilings. The high-emissivity membrane serves as radiant cooling surface instead of low-emissivity chilled ceiling so as to improve radiant heat flux, while the high transparent membrane permits great radiant heat from cooling load to chilled ceiling. A combined heat transfer analysis based on semi-transparent surface radiation and natural convection were carried out to predict cooling capacity of condensation-free radiant cooling. The results indicate that the cooling capacity could be up to 101.9W/㎡ by adding a-DIMs consisting of a high-emissivity membrane of 0.96 and a high transparent membrane of 0.87, which is improved by 2 times compared to conventional metal chilled ceiling with low emissivity of 0.2. Moreover, the cooling capacity by adding a-DIMs is further improved by 25% compared to that by using both infrared transparent DIMs presented in our previous work. The results also indicate that the cooling capacity could be improved by above 2 times compared to conventional low-emissivity metal chilled ceiling by using the radiant cooling with a-DIMs for various humidity. It will be of great guidance for high-performance radiant cooling design without condensation and improved cooling capacity for low-emissivity metal chilled ceiling.
Patterns of Gastrointestinal Helminth Infections in Rattus rattus, Rattus norvegicus, and Mus musculus in Chile
Authors: Grandón-Ojeda Alexandra , Moreno Lucila , Garcés-Tapia Carolina , Figueroa-Sandoval Fernanda , Beltrán-Venegas Jazmín , Serrano-Reyes Josselyn , Bustamante-Garrido Bárbara , Lobos-Chávez Felipe , Espinoza-Rojas Hellen , Silva-de la Fuente María Carolina , Henríquez AnaLía , Landaeta-Aqueveque Carlos
Published: June 2022

Few studies have assessed the patterns of parasite populations of rodents over a longitudinal gradient in Chile. In this work, the gastrointestinal helminthic fauna of invasive rodents in Chile was examined to assess the association between their presence/absence and abundance with latitude, host sex, and host body condition, and to assess the coexistence and correlation of the abundance between parasite species. Rodents were obtained from 20 localities between 33 and 43°S. Helminths were extracted from the gastrointestinal tract and identified morphologically. Overall, 13 helminth taxa were obtained. The most frequently identified parasite species wasHeterakis spumosa, and the most abundant wasSyphacia muris, whilePhysalopterasp. was the most widely distributed. No locality presented with a coexistence that was different from that expected by chance, while the abundance of five helminthic species correlated with the abundance of another in at least one locality, most likely due to co-infection rather than interaction. Host sex was associated with parasite presence or abundance, and female sex-biased parasitism was notably observed in all cases. Body condition and latitude presented either a positive or negative association with the presence or abundance of parasites depending on the species. It is notable that the likely nativePhysalopterasp. is widely distributed among invasive rodents. Further, gravid females were found, suggesting spillback of this species to the native fauna. The low frequency and abundance of highly zoonotic hymenolepid species suggest that rodents are of low concern regarding gastrointestinal zoonotic helminths.
Effects of Medium-Chain Fatty Acid Glycerides on Nutrient Metabolism and Energy Utilization in Weaned Piglets
Authors: Cui Zhijuan , Wang Xianze , Liao Simeng , Qi Ming , Zha Andong , Zuo Gang , Liao Peng , Chen Yuguang , Guo Chun , Tan Bie
Published: June 2022

Weaning stress induces the depressed digestive and absorptive capacity and insufficient intestinal energy supply. Medium-chain fatty acid glycerides have shown to improve the growth performance and intestinal barrier function of weaned piglets in the previous study. This study was aimed to investigate the regulation of medium-chain fatty acid glyceride on the nutrient absorption and energy utilization of weaned piglets. Nighty healthy weaned piglets were randomly assigned into five treatments: NP (Normal protein, normal-protein diet no antibiotics included); NC (Negative control, low-protein diet no antibiotics included); PC (Positive control, low-protein diet +75 mg/kg quinocetone, 20 mg/kg virginiamycin and 50 mg/kg aureomycin); MCT (tricaprylin + tricaprin group, low-protein diet + tricaprylin + tricaprin); GML (glycerol monolaurate group, low-protein diet + glycerol monolaurate). The results showed that GML treatment increased the ALP activity, concentrations of serine and methionine, MCT treatment increased concentrations of serine and 3-methyl-histidine but decreased TG concentration in serum. MCT and GML supplementations significantly promoted the lipase activity in the jejunum and ileum, as well as the AMP content in the ileal mucosa. GML addition significantly decreased the contents of butyric acid, isobutyric acid and total volatile fatty acid. In addition, medium chain fatty acid glycerides altered gene expressions involved in lipid metabolism, which showing the increases of AMPK2, CD36 and CGI58 and the decreases of MGAT2 and DGAT2 in the liver, as well as the increases of CD36, CGI58, MGAT2 and DGAT2 in the subcutaneous adipose tissue. These findings showed that medium-chain fatty acid glyceride can effectively improve the absorption of nutrients and lipid metabolism of piglets to meet the energy demand of weaned piglets, and then regulate the growth and development of weaned piglets.
Toward a Functional Cure for HIV-1 Infection: The Block and Lock Therapeutic Approach
Authors: Vargas Benni , Sluis-Cremer Nicolas
Published: June 2022

The persistence of latent, replication-competent HIV-1 proviruses in resting CD4+ T cells, and other cellular reservoirs, represents a major barrier to a cure. This reservoir is impervious to the immune system and to antiretroviral therapy, but has the potential to produce infectious rebound virus if antiretroviral therapy is interrupted. There are multiple ongoing efforts to identify and/or develop novel therapeutic strategies to eliminate or silence this latent reservoir of HIV-1 infection. One of these strategies is termed “block and lock”. The “block” refers to a therapeutic agent’s capacity to inhibit (or “block”) transcription of HIV-1 proviruses, while the “lock” refers to its capacity to induce permanent silencing of the proviruses, typically via repressive epigenetic modifications. The “block and lock” approach elicits a functional, rather than sterilizing, cure for HIV-1 infection. This review article focuses on therapeutic approaches (i.e., small molecules, nucleic acids and recombinant proteins) that have been identified to block and, in some cases, lock HIV-1 in the latent state. We also touch on critical research that needs to be accomplished to advance this approach into humans.
Parametric Research and Aerodynamic Characteristic of a Two-Stage Transonic Compressor for a Turbine Based Combined Cycle Engine
Authors: Shi Hengtao
Published: June 2022

This paper researches the parametric optimization of a two-stage transonic compressor having a large air bypass at partial rotating speed according to flow analysis for a turbine-based combined cycle engine (TBCC). To obtain adequate thrust, the inlet transonic compressor of the turbofan part of the TBCC is required to have a wider frequently used corrected rotating speed range and a larger mass-flow rate at low rotating speed, which is different from a typical transonic compressor. The one-dimensional blade design parameters and flow path of the baseline two-stage transonic compressor are introduced. With the widely used CFD software Numeca, the three-dimensional flow fields of the baseline transonic compressor and effects of the flow path between Stage 1 and Stage 2 on the inlet mass flow rate are analyzed for indicating the further improvement direction. For design speed (NC = 1.0), to improve the efficiency at the design point, parametric research is carried out on Rotor 2 to optimize the shock structure and strength, resulting in enhanced efficiency at the design point due to reduced shock loss of Rotor 2. For partial speed (NC = 0.8 and 0.7), since the flow field analysis indicates that the flow blockage in S1 limits the entire mass flow rate, the parametric redesign of stator S1 aims at obtaining an increased blade throat width to enhance the flow capacity of S1. Simulation confirms the increase in the mass-flow rate and efficiency at partial speed due to the reduction in flow blockage and related viscous losses. Aerodynamic analysis at representative operation points indicates that the modifications of R2 and S1 lead to obvious aerodynamic improvement at all rotating speeds (NC = 1.0 to 0.7), while maintaining sufficient stall margin.
Nitrogen and Phosphorus Concentrations and Their Ratios as Indicators of Water Quality and Eutrophication of the Hydro-System Danube–Tisza–Danube
Authors: Savic Radovan , Stajic Milica , Blagojević Boško , Bezdan Atila , Vranesevic Milica , Nikolić Jokanović Vesna , Baumgertel Aleksandar , Bubalo Kovačić Marina , Horvatinec Jelena , Ondrasek Gabrijel
Published: June 2022

Conserving clean and safe freshwater is a global challenge, with nitrogen (N) and phosphorus (P) as frequent limiting factors affecting water quality due to eutrophication. This paper provides a critical overview of the spatiotemporal variability in both nutrient concentrations and their total mass ratio (TN:TP) in the canal network of the Hydro system Danube–Tisza–Danube at 21 measuring locations monitored by the Environmental Protection Agency of the Republic of Serbia over a length of almost 1000 km, collected once a month during the last decade. A spatiotemporal variation in nutrient concentrations in the tested surface water samples was confirmed by correlations and cluster analyses. The highest TN concentrations were found in winter and early spring (non-vegetation season), and the highest TP concentrations in the middle of the year (vegetation season). The TN:TP mass ratio as an indicator of the eutrophication pointed out N and P co-limitation (TN:TP 8–24) in 64% of samples, N limitation (TN:TP < 8) was detected in 27% and P limitation (TN:TP > 24) in the remaining 9% of water samples. Such observations indicate slow-flowing, lowland water courses exposed to the effects of non-point and point contamination sources as nutrient runoff from the surrounding farmlands and/or urban and industrial zones, but further investigation is needed for clarification. These results are an important starting point for reducing N and P runoff loads and controlling source pollution to improve water quality and underpin recovery from eutrophication in the studied watershed.
Green Manure Amendment in Paddies Improves Soil Carbon Sequestration but Cannot Substitute the Critical Role of N Fertilizer in Rice Production
Authors: Hou Pengfu , Xue Lixiang , Wang Jing , Petropoulos Evangelos , Xue Lihong , Yang Linzhang
Published: June 2022

Clarifying the benefits of carbon sequestration and crop production of continuous green manure amendment is crucial for sustainable agricultural development. Here, using a long-term located experiment, we assessed the effects of 18 years’ ryegrass/milk vetch amendment with (NF, 150 kg N ha−1) or without nitrogen (N) fertilizer input (CK), on soil carbon management indices, paddy methane (CH4) emissions and rice yields. The results showed that green manure, rather than fertilization, played a critical role in soil CMI, increasing the carbon pool index but reducing the carbon management index. The increased soil organic carbon and the reduced labile organic carbon were the main causes for this performance. Additionally, the effects of both fertilization and green manure amendment on CH4 emissions were insignificant; however, fertilization significantly increased grain yield by 39.30% compared to CK. As a result, the methane emission intensity under fertilization treatment was notably lower than that from CK. The findings suggest that green manure amendment is a profitable manipulation for enhancing carbon sequestration without increasing paddy CH4 emissions. However, this cannot substitute the critical role of N fertilizer in rice production.
The Impact of Sowing Date on Soil Mineral Nitrogen Uptake Efficiency and Fertilizer N Uptake Efficiency for Winter Oilseed Rape (Brassica napus L.) in Ireland
Authors: Rahimitanha Shiva , Woodcock Tony , Spink John , Forristal Patrick Dermot , Berry Peter Michael
Published: June 2022

Nitrogen (N) application on winter oilseed rape (Brassica napus L.; WOSR) in the mild Atlantic climate of Ireland is based on a soil N Index system, which does not take into account any variations in crop N demand prior to the main spring dressing of N fertilizer. This study tests whether UK- canopy management (CM) principles for oilseed rape N fertilization are applicable for oilseed rape grown in Ireland. The tested principles included (i) final N uptake of unfertilized crops will be equivalent to the soil mineral N (SMN) and N in the crop measured at the end of winter, i.e., soil N uptake efficiency (SNUpE) = 1; and (ii) the (apparent) N fertilizer N uptake efficiency, (FNUpE) is 0.6 (60%). Three years of field trials were carried out from 2017/18 to 2019/20 on different sites based on a split-plot design. Three sowing dates (SD): mid-August (SD1), End-August (SD2) and mid-September (SD3) were set as main plots and five N application strategies namely, CM standard, CM higher yield, CM low N rate, Fix225 and zero-N were set at subplot level. Results from unfertilized plots demonstrated that the ratio of final crop N uptake at harvest to the combined post-winter SMN + crop N was 1.13 and 1.14 on the two early sowing dates but 1.68 for the latest sowing. Additionally, SMN was not systematically impacted by SD, except in site-year-2. Instead, crop N content in spring had the biggest influence when calculating soil N supply for the season. More mineralization occurred through the growing season resulting in SNUpE of above 100% on all SDs. This additional available N (AAN) was quantified as 11 kgN·ha−1 on average for SD1 and SD2 and 38.6 kgN·ha−1 for SD3. FNUpE values were calculated closest to the economical optimum N rate (Nopt) and were 0.534, 0.574 and 0.486, respectively for SD1, SD2 and SD3. The Nopts at each SD were 157 kgN·ha−1 (SD1), 148 kgN·ha−1 (SD2) and 175 kgN·ha−1 (SD3) and the respective yields at each Nopt were 4.93 t ha−1, 4.90 t·ha−1 and 4.34 t·ha−1. This study shows the UK–CM principles were applicable in the mild Irish climate; however, values of SNUpE and FNUpE differ from one site-year-SD to another. Mid-August to early September sowing dates produced higher post-winter crop N content due to SMN uptake, and this results in a lower Nopt than the later SD. Sowing later led to a higher Nopt due to a combination of lower post-winter N uptake and lower FNUpE, although the effect of these factors was partially offset by a greater AAN. Measurements of SNS post-winter, an estimate of N mineralization during the growing season, together with a consideration of the sowing date helped determine the scope for reduction in fertilizer while achieving high yield and high FNUpE. Among different N rate strategies, CM standard and CM high yield were closest to the Nopt for having lower N rates at the maximum economical yield point.
Rice Genetics: Trends and Challenges for the Future Crops Production
Authors: Kang Kwon-Kyoo , Cho Yong-Gu
Published: June 2022

Twenty-first-century agriculture faces serious challenges in every country on the planet due to global population growth, declining genetic resources, climate change, farmland loss due to urbanization, and stagnant crop yields [...]
Efficiency of Phosphorus Use in Sunflower
Authors: de Oliveira Anna Kézia Soares , Soares Enielson Bezerra , dos Santos Manoel Galdino , Lins Hamurábi Anizio , de Freitas Souza Matheus , dos Santos Coêlho Ester , Silveira Lindomar Maria , Mendonça Vander , Barros Júnior Aurélio Paes , de Araújo Rangel Lopes Welder
Published: June 2022

Sunflower is an oleaginous plant of great importance worldwide that stands out in the production of edible oil and human food. The identification of differences between cultivars regarding the use of phosphorus and the production of achenes at different levels of phosphate fertilization is a viable strategy to allow its cultivation in soils with different concentrations of phosphorus, without compromising yield and reducing environmental damage associated with excessive applications of phosphate fertilizers. Thus, the objective of this study was to evaluate different levels of phosphorus fertilization and sunflower cultivars regarding the efficiency of phosphorus use in two agricultural crops. The experimental design was in randomized blocks with four replications in split-plots, where four doses of phosphorus (50, 100, 150, and 200 kg ha−1 of P2O5) were allocated in the plots, and in the subplots three sunflower cultivars (Aguará 06, Altis 99, and BRS 122). The phosphorus use efficiency of sunflower cultivars was estimated through agronomic efficiency, vegetative efficiency, agrophysiological efficiency, apparent recovery efficiency, and utilization efficiency. The dose of 50 kg ha−1 of P2O5 provided the best phosphorus efficiency indices for the different sunflower cultivars in the two studied crops. Cultivar Aguará 06 was more efficient in conditions with P2O5 supply below 150 kg ha−1, regardless of the crop. Sunflower cultivars had the best efficiency indices of phosphorus applied in the 2016 harvest.
In Silico Docking, Resistance Modulation and Biofilm Gene Expression in Multidrug-Resistant Acinetobacter baumannii via Cinnamic and Gallic Acids
Authors: Abdelaziz Neveen A. , Elkhatib Walid F. , Sherif Mahmoud M. , Abourehab Mohammed A. S. , Al-Rashood Sara T. , Eldehna Wagdy M. , Mostafa Nada M. , Elleboudy Nooran S.
Published: June 2022

Despite the mounting global burden of antimicrobial resistance (AMR), the generation of new classes of effective antimicrobials still lags far behind. The interplay between multidrug resistance and biofilm formation in Acinetobacter baumannii has drastically narrowed the available therapeutic choices. The use of natural compounds holds promise as an alternate option for restoring the activity of existing antibiotics and attenuating virulence traits through reduced biofilm formation. This study aimed to evaluate the modulatory effect of combining cinnamic and gallic acids at ½MIC with various antibiotics against multidrug-resistant (MDR) A. baumannii clinical isolates as well as study the effect on the expression of the biofilm-associated genes (bap, csuE, ompA) via quantitative, real-time PCR. Combining cinnamic or gallic acid with imipenem, amikacin or doxycycline resulted in significant reduction of resistance (p < 0.05). On the contrary, no effect was recorded when both acids were combined with levofloxacin, and only cinnamic acid had a synergistic effect with colistin. The transcriptomic changes of biofilm-related genes in the presence of gallic acid at ½MIC were compared with untreated control samples. The fold expression values proved that gallic acid substantially down-regulated the respective genes in all five strong biofilm formers. Molecular docking studies of gallic and cinnamic acids on target genes revealed good binding affinities and verified the proposed mechanism of action. To the best of our knowledge, this is the first report on the effect of gallic acid on the expression of bap, csuE and ompA genes in A. baumannii, which may permit its use as an adjunct anti-virulence therapeutic strategy.
The Effect of Tauroursodeoxycholic Acid (TUDCA) Treatment on Pregnancy Outcomes and Vascular Function in a Rat Model of Advanced Maternal Age
Authors: Pasha Mazhar , Kirschenman Raven , Wooldridge Amy , Spaans Floor , Cooke Christy-Lynn M. , Davidge Sandra T.
Published: June 2022

Advanced maternal age (≥35 years) increases the risk of vascular complications in pregnancy that can result in fetal growth restriction and preeclampsia. Endoplasmic reticulum (ER) stress has been linked to adverse pregnancy outcomes in these complicated pregnancies. However, the role of ER stress in advanced maternal age is not known. We hypothesize that increased ER stress contributes to altered vascular function and poor pregnancy outcomes, and that treatment with the ER-stress inhibitor TUDCA will improve pregnancy outcomes. First, young and aged non-pregnant/pregnant rats were used to assess ER stress markers in mesenteric arteries; mesenteric artery phospho-eIF2α and CHOP expression were increased in aged dams compared to young dams. In a second study, young and aged control and TUDCA-treated dams were studied on gestational day (GD) 20 (term = 22 days). TUDCA treatment was provided via the drinking water throughout pregnancy (GD0-GD20; calculated dose of 150 mg/kg/day TUDCA). ER stress markers were quantified in mesenteric arteries, blood pressure was measured, pregnancy outcomes were recorded, mesenteric and main uterine arteries were isolated and vascular function was assessed by wire myography. Aged dams had increased phospho-eIF2α and CHOP expression, reduced fetal weight, reduced litter size, and impaired uterine artery relaxation. In the aged dams, TUDCA treatment reduced phospho-eIF2α and CHOP expression, reduced blood pressure, improved fetal body weight, and tended to improve uterine artery function compared to control-treated aged dams. In conclusion, our data illustrate the role of ER stress, as well as TUDCA as a potential therapeutic that may benefit pregnancy outcomes in advanced maternal age.
Zebrafish Models to Study the Crosstalk between Inflammation and NADPH Oxidase-Derived Oxidative Stress in Melanoma
Authors: Pardo-Sánchez Irene , García-Moreno Diana , Mulero Victoriano
Published: June 2022

Melanoma is the deadliest form of skin cancer, and its incidence continues to increase. In the early stages of melanoma, when the malignant cells have not spread to lymph nodes, they can be removed by simple surgery and there is usually low recurrence. Melanoma has a high mortality rate due to its ability to metastasize; once melanoma has spread, it becomes a major health complication. For these reasons, it is important to study how healthy melanocytes transform into melanoma cells, how they interact with the immune system, which mechanisms they use to escape immunosurveillance, and, finally, how they spread and colonize other tissues, metastasizing. Inflammation and oxidative stress play important roles in the development of several types of cancer, including melanoma, but it is not yet clear under which conditions they are beneficial or detrimental. Models capable of studying the relevance of inflammation and oxidative stress in the early steps of melanocyte transformation are urgently needed, as they are expected to help recognize premetastatic lesions in patients by improving both early detection and the development of new therapies.
Hepatic Myofibroblasts: A Heterogeneous and Redox-Modulated Cell Population in Liver Fibrogenesis
Authors: Bocca Claudia , Protopapa Francesca , Foglia Beatrice , Maggiora Marina , Cannito Stefania , Parola Maurizio , Novo Erica
Published: June 2022

During chronic liver disease (CLD) progression, hepatic myofibroblasts (MFs) represent a unique cellular phenotype that plays a critical role in driving liver fibrogenesis and then fibrosis. Although they could originate from different cell types, MFs exhibit a rather common pattern of pro-fibrogenic phenotypic responses, which are mostly elicited or sustained both by oxidative stress and reactive oxygen species (ROS) and several mediators (including growth factors, cytokines, chemokines, and others) that often operate through the up-regulation of the intracellular generation of ROS. In the present review, we will offer an overview of the role of MFs in the fibrogenic progression of CLD from different etiologies by focusing our attention on the direct or indirect role of ROS and, more generally, oxidative stress in regulating MF-related phenotypic responses. Moreover, this review has the purpose of illustrating the real complexity of the ROS modulation during CLD progression. The reader will have to keep in mind that a number of issues are able to affect the behavior of the cells involved: a) the different concentrations of reactive species, b) the intrinsic state of the target cells, as well as c) the presence of different growth factors, cytokines, and other mediators in the extracellular microenvironment or of other cellular sources of ROS.
An Underdetermined Convolutional Blind Separation Algorithm for Time–Frequency Overlapped Wireless Communication Signals with Unknown Source Number
Authors: Ma Hao , Zheng Xiang , Yu Lu , Wu Xinrong , Zhang Yu
Published: June 2022

It has been challenging to separate the time–frequency (TF) overlapped wireless communication signals with unknown source numbers in underdetermined cases. In order to address this issue, a novel blind separation strategy based on a TF soft mask is proposed in this paper. Based on the clustering property of the signals in the sparse domain, the angular probability density distribution is obtained by the kernel density estimation (KDE) algorithm, and then the number of source signals is identified by detecting the peak points of the distribution. Afterward, the contribution degree function is designed according to the cosine distance to calculate the contribution degrees of the source signals in the mixed signals. The separation of the TF overlapped signals is achieved by constructing a soft mask matrix based on the contribution degrees. The simulations are performed with digital signals of the same modulation and different modulation, respectively. The results show that the proposed algorithm has better anti-aliasing and anti-noise performance than the comparison algorithms.
A Photoplethysmogram Dataset for Emotional Analysis
Authors: Jin Ye-Ji , Habibilloh Erkinov , Jang Ye-Seul , An Taejun , Jo Donghyun , Park Saron , Chang Won-Du
Published: June 2022

In recent years, research on emotion classification based on physiological signals has actively attracted scholars’ attention worldwide. Several studies and experiments have been conducted to analyze human emotions based on physiological signals, including the use of electrocardiograms (ECGs), electroencephalograms (EEGs), and photoplethysmograms (PPGs). Although the achievements with ECGs and EEGs are progressive, reaching higher accuracies over 90%, the number of studies utilizing PPGs are limited and their accuracies are relatively lower than other signals. One of the difficulties in studying PPGs for emotional analysis is the lack of open datasets (there is a single dataset to the best of the authors). This study introduces a new PPG dataset for emotional analysis. A total of 72 PPGs were recorded from 18 participants while watching short video clips and analyzed in time and frequency domains. Moreover, emotional classification accuracies with the presented dataset were presented with various neural network structures. The results prove that this dataset can be used for further emotional analysis with PPGs.
Quantifying Within-Individual Elbow Load Variability in Youth Elite Baseball Pitchers and Its Role in Overuse Injuries
Authors: Van Trigt Bart , Bouman Foskien F. , Leenen Antonius J. R. , Hoozemans Marco J. M. , Van der Helm Frans C. T. , Veeger Dirkjan
Published: June 2022

Medial elbow overuse injuries are rising in baseball. The external valgus torque magnitude is a possible risk factor for medial elbow injuries. The magnitude on its own cannot explain why one pitcher sustains an injury and another does not. Therefore, the aim of this study is to describe the within-individual external valgus torque variability and to determine whether the within-individual external valgus torque variability can be described by a Gaussian distribution. Eleven youth elite baseball pitchers threw twenty-five fastball pitches. Body kinematics were measured with VICON motion capture at 400 Hz. Elbow valgus torques of the total 270 pitches were calculated with a custom-made inverse dynamic model in Python. Visual inspection and the Shapiro–Wilk test were performed to test for the within-individual elbow valgus torque normality. The results showed that within-individual valgus torque variability was present in pitchers and differed among pitchers. Furthermore, it was shown that the within-individual valgus torque variability was normally distributed in nine out of eleven subjects. In conclusion, the presence of and differences in within-individual elbow load variability among baseball pitchers can be useful variables as they might be related to overuse elbow injuries.
Visual Tracking with FPN Based on Transformer and Response Map Enhancement
Authors: Deng Anping , Liu Jinghong , Chen Qiqi , Wang Xuan , Zuo Yujia
Published: June 2022

Siamese network-based trackers satisfy the balance between performance and efficiency for visual tracking. However, they do not have enough robustness to handle the challenges of target occlusion and similar objects. In order to improve the robustness of the tracking algorithm, this paper proposes visual tracking with FPN based on Transformer and response map enhancement. In this paper, a feature pyramid structure based on Transformer is designed to encode robust target-specific appearance features, as well as the response map enhanced module to improve the tracker’s ability to distinguish object and background. Extensive experiments and ablation experiments are conducted on many challenging benchmarks such as UAV123, GOT-10K, LaSOT and OTB100. These results show that the tracking algorithm we proposed in this paper can effectively improve the tracking robustness against the challenges of target occlusion and similar object, and thus improve the precision rate and success rate of the tracking algorithm.
Research on Long Text Classification Model Based on Multi-Feature Weighted Fusion
Authors: Yue Xi , Zhou Tao , He Lei , Li Yuxia
Published: June 2022

Text classification in the long-text domain has become a development challenge due to the significant increase in text data, complexity enhancement, and feature extraction of long texts in various domains of the Internet. A long text classification model based on multi-feature weighted fusion is proposed for the problems of contextual semantic relations, long-distance global relations, and multi-sense words in long text classification tasks. The BERT model is used to obtain feature representations containing global semantic and contextual feature information of text, convolutional neural networks to obtain features at different levels and combine attention mechanisms to obtain weighted local features, fuse global contextual features with weighted local features, and obtain classification results by equal-length convolutional pooling. The experimental results show that the proposed model outperforms other models in terms of accuracy, precision, recall, F1 value, etc., under the same data set conditions compared with traditional deep learning classification models, and it can be seen that the model has more obvious advantages in long text classification.
An Evidence-Based Survey on Full-Scale Membrane Biological Reactors: Main Technical Features and Operational Aspects
Authors: Vaccari Mentore , Abbà Alessandro , Bertanza Giorgio , Collivignarelli Carlo
Published: June 2022

This paper presents the results of a survey on full-scale membrane biological reactors (MBRs) wastewater treatment plants (WWTPs) in Italy. Alongside the main technical characteristics of the Italian MBR plants, the opinions of the plant managers on the operational advantages and disadvantages are described. As reported by the MBR technology suppliers, approximately 290 MBR municipal or industrial WWTPs are in operation in Italy, out of which 242 were studied in this survey. Data from more than one hundred municipal WWTPs were collected; these account for a total capacity of about 2,000,000 population equivalent (PE), which corresponds to 3% of the total organic load treated by the Italian WWTPs with secondary and advanced treatment. Usually, small installations adopt the flat-sheet rather than hollow-fiber membrane configuration. The main reasons why the MBR technology has been preferred to other options are its potential to be used for increasing the treatment capacity of existing plants and its compactness. Moreover, the followed operational advantages have been highlighted: easiness to comply with the discharge limits, removal of pathogens without specific disinfection units, possibility of internal reuse of the effluent, and process automation. Membrane fouling and plant shutdown have been recorded as the most relevant troubles, the last one indeed occurring only occasionally or rarely.
Characterization and Evaluation of Commercial Carboxymethyl Cellulose Potential as an Active Ingredient for Cosmetics
Authors: Costa Eduardo M. , Pereira Carla F. , Ribeiro Alessandra A. , Casanova Francisca , Freixo Ricardo , Pintado Manuela , Ramos Oscar L.
Published: June 2022

Carboxymethyl cellulose is the most used water-soluble cellulose with applications in industries such as food, cosmetics, and tissue engineering. However, due to a perceived lack of biological activity, carboxymethyl cellulose is mostly used as a structural element. As such, this work sought to investigate whether CMC possesses relevant biological properties that could grant it added value as a cosmeceutical ingredient in future skincare formulations. To that end, CMC samples (Mw between 471 and 322 kDa) skin cell cytotoxicity, impact upon pro-collagen I α I production, and inflammatory response were evaluated. Results showed that samples were not cytotoxic towards HaCat and HDFa up to 10 mg/mL while simultaneously promoting intracellular production of pro-collagen I α I up by 228% relative to the basal metabolism, which appeared to be related to the highest DS and Mw. Additionally, CMC samples modulated HaCat immune response as they decreased by ca. 1.4-fold IL-8 production and increased IL-6 levels by ca. five fold. Despite this increase, only two samples presented IL-6 levels similar to those of the inflammation control. Considering these results, CMC showed potential to be a more natural alternative to traditional bioactive cosmetic ingredients and, as it is capable of being a bioactive and structural ingredient, it may play a key role in future skincare formulations.