bluuebunny/crossref_metadata_2025 huggingface dataset:
New Results on Robust Synchronization for Memristive Neural Networks with Fractional Derivatives via Linear Matrix Inequality
Authors: Song Chao , Cao Jinde , Abdel-Aty Mahmoud
Published: October 2022

This article mainly concentrates on the synchronization problem for a more general kind of the master–slave memristor-based neural networks with fractional derivative. By applying a continuous-frequency-distributed equivalent model tool, some new outcomes and sufficient conditions on the robust synchronization of the master–slave neural networks with uncertainty are proposed via linear matrix inequality (LMI). Finally, two memristive neural networks model with fractional derivatives are presented to validate the efficiency of the theoretical results.
Insights into the Role of Natural Polysaccharide-Based Hydrogel Wound Dressings in Biomedical Applications
Authors: Sun Ying , Li Duanxin , Yu Yang , Zheng Yongjie
Published: October 2022

Acute skin damage caused by burns or cuts occurs frequently in people’s daily lives. Such wounds are difficult to heal normally and have persistent inflammation. Wound dressings not only improve the speed of wound healing, but also protect and cover the wound well. Hydrogels have the characteristics of good flexibility, high water content, and good biocompatibility, and are widely used in biomedicine and other fields. Common hydrogels are mainly natural hydrogels and synthetic hydrogels. Hydrogels cross-linked using different raw materials and different methods have different performance characteristics. Natural hydrogels prepared using polysaccharides are simple to obtain and have good biocompatibility, but are inferior to synthetic hydrogels in terms of mechanical properties and stability, and a single polysaccharide hydrogel cannot meet the component requirements for wound healing. Therefore, functional composite hydrogels with high mechanical properties, high biocompatibility, and high antibacterial properties are the current research hotspots. In this review, several common polysaccharides for hydrogel synthesis and the synthesis methods of polysaccharide hydrogels are introduced, and functional composite hydrogel dressings from recent years are classified. It is hoped that this can provide useful references for relevant research in this field.
The Function of BBX Gene Family under Multiple Stresses in Nicotiana tabacum
Authors: Song Kangkang , Li Bin , Wu Hui , Sha Yuxue , Qin Liting , Chen Xingyun , Liu Ying , Tang Heng , Yang Long
Published: October 2022

B-box (BBX) is a zinc finger transcription factor, which is involved in regulating the growth and development of plants and resisting various stresses. In this study, 43 NtBBX genes were identified and divided into five subgroups in tobacco. The members in each subgroup had similar characteristics. The promoter region of NtBBX genes had cis-acting elements related to light response, hormone regulation and stress response. Transcriptome analysis showed that NtBBX30 was significantly up-regulated, and NtBBX12, NtBBX13, NtBBX16 and NtBBX17 were significantly down-regulated under abiotic stresses. The NtBBX genes also responded to the infection of Ralstonia solanacearum. NtBBX9, NtBBX1, NtBBX15 and NtBBX17 showed the greatest response under stresses. The NtBBX genes are expressed in various degrees under different tissues. This research will provide a solid foundation for further study of the biological function of NtBBX genes in tobacco.
Evaluation of the Maturity and Gene Expression of Sheep Oocytes and Embryos Cultured in Media Supplemented with Marjoram (Origanum vulgare) Extract
Authors: Alotaibi Mashael M. , Alhimaidi Ahmad R. , Al-Ghadi Muath Q. , Ammari Aiman A. , Al-Malahi Nawal M.
Published: October 2022

This study aimed to evaluate the effects of marjoram extract on oocyte maturation and gene expression in sheep oocytes and embryos. The first experiment studied the effect of the extract as an antioxidant to improve the in vitro maturation media used for sheep oocytes; the oocytes were matured in a TCM199 medium supplemented with 1 or 10 µg/mL of marjoram extract or the control, 0 µg, for 24 hr. Then, the maturation was estimated, and the gene expression was measured by using qPCR. The second experiment studied the effect of the extract on the development of sheep embryos produced in vitro; the fertilized oocytes were cultured in a SOF medium supplemented with 1 or 10 µg/mL of marjoram extract or the control, 0 µg, for 7 days. Then, the gene expression was measured using qPCR. The results showed that the marjoram extract did not improve nuclear maturation or the blastocyst rate. There was a significant increase in the level of GDF-9 gene expression in mature oocytes in the treatment groups. An increase in the expression of BCL-2 and EGR-1 genes was observed for the blastocysts in the 10 µg/mL group. We concluded that the marjoram extract did not improve nuclear maturation, but it did affect the expression of some genes in sheep oocytes and embryos.
Transformed Waldenström Macroglobulinemia: Update on Diagnosis, Prognosis and Treatment
Authors: Durot Eric , Tomowiak Cécile , Toussaint Elise , Morel Pierre , Talaulikar Dipti , Kapoor Prashant , Castillo Jorge J. , Delmer Alain
Published: October 2022

Histological transformation (HT) to an aggressive lymphoma results from a rare evolution of Waldenström macroglobulinemia (WM). A higher incidence of transformation events has been reported in MYD88 wild-type WM patients. HT in WM can be histologically heterogeneous, although the diffuse large B-cell lymphoma of activated B-cell subtype is the predominant pathologic entity. The pathophysiology of HT is largely unknown. The clinical suspicion of HT is based on physical deterioration and the rapid enlargement of the lymph nodes in WM patients. Most transformed WM patients present with elevated serum lactate dehydrogenase (LDH) and extranodal disease. A histologic confirmation regarding the transformation to a higher-grade lymphoma is mandatory for the diagnosis of HT, and the choice of the biopsy site may be dictated by the findings of the 18fluorodeoxyglucose-positron emission tomography/computed tomography. The prognosis of HT in WM is unfavorable, with a significantly inferior outcome compared to WM patients without HT. A validated prognostic score based on 3 adverse risk factors (elevated LDH, platelet count < 100 × 109/L and any previous treatment for WM) stratifies patients into 3 risk groups. The most common initial treatment used is a chemo-immunotherapy (CIT), such as R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, prednisone). The response duration is short and central nervous system relapses are frequent. Whether autologous stem cell transplantation could benefit fit patients responding to CIT remains to be studied.
Active Carboxymethyl Cellulose-Based Edible Coatings for the Extension of Fresh Goldenberries Shelf-Life
Authors: Ballesteros Lina F. , Teixeira José A. , Cerqueira Miguel A.
Published: October 2022

Edible coatings based on natural sources are a good alternative to protect and increase the shelf-life of the fruits. In this work, active carboxymethyl cellulose (CMC)-based edible coatings enriched with functional ingredients, extracted from spent coffee grounds (SCG), were produced and used to increase the shelf-life of fresh goldenberries. Thus, three different treatments were tested on fresh goldenberries: (i) coating CMC (CMC-based edible coating); (ii) coating CMC-A (CMC-based edible coating with 0.2% (w/v) of polysaccharide-rich extract from SCG); and (iii) coating CMC-B (CMC-based edible coating with 0.2% (w/v) of polysaccharide-rich extract, and 0.2% (w/v) of phenolic compounds encapsulated from SCG), being compared with uncoated goldenberries. Physicochemical and microbiological properties of the fruits were evaluated throughout 12 and 28 days of storing at 20 °C and 65% relative humidity and 4 °C and 95% RH, respectively. The results showed that the temperature and relative humidity significantly influenced the quality of goldenberries, increasing their shelf-life almost two-fold when stored at 4 °C and 95% RH. Both CMC-A and CMC-B coatings controlled weight loss and decreased the gas transfer rates (O2, CO2, and C2H4), mainly when goldenberries were stored at 20 °C and 65% RH. Moreover, coating CMC-A was better for delaying microbial growth, while the edible coating CMC-B gave an extra content of phenolic compounds to goldenberries, increasing thus their antioxidant potential.
Tomato Landraces May Benefit from Protected Production—Evaluation on Phytochemicals
Authors: Divéky-Ertsey Anna , Ladányi Márta , Biró Barbara , Máté Mónika , Drexler Dóra , Tóth Ferenc , Boziné Pullai Krisztina , Gere Attila , Pusztai Péter , Csambalik László
Published: October 2022

Plant genetic resources (PGRs) serving as a wide genetic pool of tomato germplasm can provide a solid base for recent breeding efforts to increase consumer acceptance towards the taste and the phytonutrient properties of novel tomato varieties. Old varieties and landraces were abandoned by producers due to unfavorable phenotypic characteristics; however, their high adaptability and nutritional properties are inevitably valuable. This study aims to investigate the impact of open-field vs. protected production on various bioactive compound parameters and on the antioxidant status of seven indeterminate-type tomato PGRs in an organic production system for two years (2015–2016). Genotype main effect plus genotype × environment interaction (GGE) biplots were created for visualizing the which-won-where concept of the PGRs investigated. The GGE analysis revealed that the phytonutrient content of certain PGRs is less dependent on location and more influenced by differences in microclimatic conditions. “Balatonboglár”, “Mátrafüred” and “Fadd” PGRs performed better in a polytunnel, while Tarnaméra provided better results in an open field. “Máriapócs” and “San Marzano” showed a relative independence from production location in terms of their measured phytonutrient values. These findings enrich the nutritional datasets of tomato landraces, which support the in situ conservation and utilization of PGRs in breeding programs.
Gingipain Genotyping as a Potential Predictor for the Assessment of Periodontal Health and Disease Condition
Authors: Kugaji Manohar , Bhat Kishore , Muddapur Uday , Joshi Vinayak , Peram Malleswara Rao , Kumbar Vijay
Published: October 2022

Oral hygiene maintenance is important to maintain optimal oral health. Oral health is affected by dysbiotic oral microflora in the dental plaque. Virulent factors of pathogenic organisms, such as gingipain, are responsible for tissue degradation and host tissue invasion in periodontal disease. We sought to investigate the distribution of gingipain genotypes (rgpA and kgp) of P. gingivalis in patients with chronic periodontitis and healthy individuals. The study included individuals positive for P. gingivalis, with 95 samples in the chronic periodontitis (CP) group and 35 samples in the healthy (H) group. We found that kgp-I and kgp-II types were prevalent in 67.36% and 32.64% of the samples in the CP group, respectively. In the H group, kgp-II was highly prevalent (97.14%). The rgpA genotype, type A was found in 78.95% and 82.85% of the samples in the CP and H group, respectively. The mean level of PD and CAL were increased in the presence of kgp-I and decreased in the presence of kgp-II. The mean level of P. gingivalis was increased in the presence of kgp-I and rgpA, type A. Our results show that kgp-I and kgp-II are strongly associated with disease and health condition, respectively.
Changes in Otorhinolaryngologic Disease Incidences before and during the COVID-19 Pandemic in Korea
Authors: Kim So Young , Yoo Dae Myoung , Kim Ji Hee , Kwon Mi Jung , Kim Joo-Hee , Chung Juyong , Choi Hyo Geun
Published: October 2022

This study aimed to investigate the change in the incidence and variance of otorhinolaryngologic diseases during the coronavirus disease 19 (COVID-19) pandemic. The entire Korean population (~50 million) was evaluated for the monthly incidence of 11 common otorhinolaryngologic diseases of upper respiratory infection (URI), influenza, acute tonsillitis, peritonsillar abscess, retropharyngeal and parapharyngeal abscess, acute laryngitis and bronchitis, stomatitis and related lesions, acute sinusitis, rhinitis, otitis media, and dizziness from January 2018 through March 2021 using the International Classification of Disease (ICD)-10 codes with the data of the Korea National Health Insurance Service. The differences in the mean incidence of 11 common otorhinolaryngologic diseases before and during COVID-19 were compared using the Mann—Whitney U test. The differences in the variance of incidence before and during COVID-19 were compared using Levene’s test. The incidence of all 11 otorhinolaryngologic diseases was lower during COVID-19 than before COVID-19 (all p < 0.05). The variations in disease incidence by season were lower during COVID-19 than before COVID-19 for infectious diseases, including URI, influenza, acute tonsillitis, peritonsillar abscess, retropharyngeal and parapharyngeal abscess, acute laryngitis and bronchitis, acute sinusitis, and otitis media (all p < 0.05), while it was not in noninfectious diseases, including stomatitis, rhinitis, and dizziness. As expected, the incidences of all otorhinolalryngolgic diseases were decreased. Additionally, we found that seasonal variations in infectious diseases disappeared during the COVID-19 pandemic, while noninfectious diseases did not.
Breastfeeding and Maternal Perceptions of Infant Sleep, Settle and Cry Patterns in the First 9 Months
Authors: Perrella Sharon Lisa , Dix-Matthews Alice , Williams Julie , Rea Alethea , Geddes Donna Tracy
Published: October 2022

This study evaluated relationships between maternal perceptions of infant sleep, settling and crying patterns and breastfeeding. A prospective observational study of 91 mothers of healthy, term infants was conducted with follow ups over 9 months after discharge from a Western Australian maternity hospital. Feeding information, sleep, settle and cry behaviours, maternal bother at infant behaviours and confidence were measured using the Sleep and Settle Questionnaire. Breastfeeding confidence was measured using the Breastfeeding Self-Efficacy Scale—Short Form. Questionnaires were administered at 2 and 6 weeks, 3, 6 and 9 months. Linear mixed models were used to assess associations between maternal bother, feeding method and infant characteristics. The feeding method was not associated with maternal bother, and cessation of breastfeeding did not result in a change in bother scores (p = 0.34). Duration of infant crying in the day, evening and night, frequency of night waking and duration of settling to sleep in the day were associated with increased bother scores. Higher breastfeeding self-efficacy and maternal confidence were associated with lower bother scores (both p < 0.01). Maternal bother is associated with infant behaviours that require parental input, but not breastfeeding status. Resources that address parental expectations regarding infant sleep while providing strategies to support maternal wellbeing and breastfeeding are needed.
Stayin’ Alive in Little 5: Application of Sentiment Analysis to Investigate Emotions of Service Industry Workers Responding to Drug Overdoses
Authors: Febres-Cordero Sarah , Smith Daniel Jackson
Published: October 2022

The opioid epidemic has increasingly been recognized as a public health issue and has challenged our current legal, social, and ethical beliefs regarding drug use. The epidemic not only impacts persons who use drugs, but also those around them, including people who do not expect to witness an overdose. For example, in the commercial district of Little 5 Points, Atlanta, GA, many service industry workers have become de facto responders to opioid overdoses when a person experiences an opioid-involved overdose in their place of employment. To provide additional insights into >300 pages of interview data collected from service industry workers that have responded to an opioid overdose while at work, we utilized a mixed-methods approach to conduct this sentiment analysis. First, using R version 4.2.1, a data-science based textual analytic approach was applied to the interview data. Using a corpus algorithm, each line of interview text was characterized as one of the eight following sentiments, anger, anticipation, disgust, fear, joy, sadness, surprise, or trust. Once having identified statements that fit into each of these eight codes, qualitative thematic analysis was conducted. The three most prevalent emotions elucidated from these interviews with service industry workers were trust, anticipation, and joy with 20.4%, 16.2%, and 14.7% across all statements, respectively labeled as each emotion. Thematic analysis revealed three themes in the data: (1) individuals have a part to address in the opioid epidemic, (2) communities have many needs related to the opioid crisis, and (3) structural forces create pathways and barriers to opioid overdose response and rescue. This analysis thematically identified roles service industry workers have in addressing the opioid crisis in Atlanta. Similarly, community needs and barriers to responding to an opioid-involved overdose were characterized. Uniquely, this study found key sentiments related to each of these themes. Future research can leverage these findings to inform the development of overdose prevention and response interventions for service industry works that systematically address common emotions and beliefs trainees may have.
Assessment of the Long-Term Mental Health Effects on Austrian Students after COVID-19 Restrictions
Authors: Kaltschik Stefan , Pieh Christoph , Dale Rachel , Probst Thomas , Pammer Barbara , Humer Elke
Published: October 2022

The mental health of adolescents has been severely affected by the COVID-19 pandemic. The aim of this study was to assess the mental health of Austrian adolescents in spring 2022, a time during which COVID-19-related restrictions had been significantly lifted. A total of N = 616 students aged between 14 and 20 participated in a cross-sectional survey between April and May 2022 (t2). The prevalence of clinically relevant symptoms was 73% among girls and 44% among boys for depression (PHQ-9 score ≥ 11), 57% in girls and 35% in boys for anxiety (GAD-7 score ≥ 11), 34% in girls and 21% in boys for sleeping problems (ISI score ≥ 15), and 95% in girls and 81% in boys for experiencing at least moderate stress (PSS-10 score ≥ 14). Frequent suicidal ideations were reported by 24% of girls and 12% of boys. These results were compared with the results from a cross-sectional study from February 2021 (t1). To account for differences in covariates between samples, data were propensity score matched before the analysis. Compared with t1, we found an increase among girls regarding clinically relevant symptoms of depression (OR = 1.78), anxiety (OR = 1.34), insomnia (OR = 1.63), and suicidal ideations (OR = 1.96; p < 0.05 for all measures). Significant correlations were found between smartphone use and mental health and physical activity and mental health for both genders. The results of this study indicated that even during the third year of the COVID-19 pandemic, the mental health of adolescents in Austria is still severely impaired.
Optimization of Work Environment and Community Labor Health Based on Digital Model—Empirical Evidence from Developing Countries
Authors: Gao Shiya , Wang Zeyu , Jiang Shaoxiang , Ding Wen , Wang Yuchen , Dong Xiufang
Published: October 2022

As far as we know, for large manufacturing enterprises, there is often a community of labor gathered around such enterprises, which is especially used as a place for the enterprise to place the labor force. This paper aimed to update the industry model of Chinese Manufacturing Enterprises (CMEs) to improve workers’ health management. This work first discusses the value, mode, and process of Enterprise Digital Transformation (EDT) and Worker Health and Safety Management (WHSM). Then, it proposes the CMEs-oriented EDT model and WHSM system based on Big Data Technology (BDT) and the Internet of Things (IoT). The proposed model and system are verified through a case study on the Shanghai BYD manufacturing enterprise (short for BYD) using the Fuzzy Comprehensive Evaluation Method (CFEM). The EDT model verification considers the adaptation and performance of enterprises after EDT. The WHSM system considers workers’ oxygen inhalation status to evaluate their heart and cardiovascular health. The results show that EDT improves the enterprise’s revenue and reshuffles the revenue structure. The EDT model has absolute adaptability to BYD. It has greatly improved BYD’s indexes, especially financial performance, market capability, and technical capability.
Impact of COVID-19 Lockdowns on the Activity and Mental Health of Older People in Indonesia: A Qualitative Study
Authors: Fauk Nelsensius Klau , Ernawati  , Dent Elsa , Asa Gregorius Abanit , Ward Paul Russell
Published: October 2022

The COVID-19 pandemic has caused detrimental impacts on different population groups throughout the world. This study aimed to explore the impacts of the COVID-19 pandemic’s mandatory lockdown protocols on individual and social activities and mental health conditions of community-dwelling older people in Jakarta, Indonesia. A qualitative design using one-on-one in-depth interviews was employed to collect data from the participants (n = 24) who were recruited using the snowball sampling technique. Data analysis was guided by the five steps proposed in a qualitative data analysis framework, including familiarisation with the data, identification of a thematic framework, indexing the data, charting the data and mapping and interpreting the data. The findings showed that before the COVID-19 outbreak participants engaged in different kinds of regular individual and social activities. However, the COVID-19 outbreak and its mandatory lockdown protocols significantly influenced both their activities and social life, which led to social disconnection and financial difficulties for them. COVID-19 outbreak, mandatory lockdown protocols, and the disruption of individual and social activities of the participants also caused mental health challenges to them, including feelings of loneliness, loss, sadness, stress, and anger. The findings suggest that there is a need for intervention programs addressing the socio-economic and mental health impacts of the COVID-19 pandemic on older populations to help them cope with these challenges. Future studies involving large-scale older populations to comprehensively understand COVID-19 impacts on them are recommended.
Application for Ecological Restoration of Contaminated Soil: Phytoremediation
Authors: Su Rongkui , Wang Yangyang , Huang Shunhong , Chen Runhua , Wang Jun
Published: October 2022

Nowadays, with the rapid development of industry and agriculture, heavy metal pollution is becoming more and more serious, mainly deriving from natural and man-made sources [...]
Evaluation of Empirical and Machine Learning Approaches for Estimating Monthly Reference Evapotranspiration with Limited Meteorological Data in the Jialing River Basin, China
Authors: Luo Jia , Dou Xianming , Ma Mingguo
Published: October 2022

The accurate estimation of reference evapotranspiration (ET0) is crucial for water resource management and crop water requirements. This study aims to develop an efficient and accurate model to estimate the monthly ET0 in the Jialing River Basin, China. For this purpose, a relevance vector machine, complex extreme learning machine (C-ELM), extremely randomized trees, and four empirical equations were developed. Monthly climatic data including mean air temperature, solar radiation, relative humidity, and wind speed from 1964 to 2014 were used as inputs for modeling. A total comparison was made between all constructed models using four statistical indicators, i.e., the coefficient of determination (R2), Nash efficiency coefficient (NSE), root mean square error (RMSE) and mean absolute error (MAE). The outcome of this study revealed that the Hargreaves equation (R2 = 0.982, NSE = 0.957, RMSE = 7.047 mm month−1, MAE = 5.946 mm month−1) had better performance than the other empirical equations. All machine learning models generally outperformed the studied empirical equations. The C-ELM model (R2 = 0.995, NSE = 0.995, RMSE = 2.517 mm month−1, MAE = 1.966 mm month−1) had the most accurate estimates among all generated models and can be recommended for monthly ET0 estimation in the Jialing River Basin, China.
“All Hands on Deck”: Psychological Characteristics of Women with Experience of Oncological Disease Participating in Sailing Cruise—A Pilot Study
Authors: Wyszomirska Julia , Bąk-Sosnowska Monika , Modrzejewska Adriana
Published: October 2022

Background: In addition to searching for effective methods of treatment, interventions are sought to support well-being, quality of life, mental health, and recovery. Sailing has its specific features, including task orientation, challenges, contact with people, and nature. This specificity may be treated as a potential therapeutic factor, but it is also likely that people with certain psychological characteristics are involved in it. Therefore, the study aimed to assess some psychological features of women with cancer experience who decided to take part in the Onco-Cruise (Polish: Onko-Rejs). Methods: Participants were 56 women (M = 46.73; SD = 9.21). We used NEO-FFI, the Zimbardo Time Perspective Inventory, and The Multidimensional Health Locus of Control Scale. Results: Onco-Cruises participants were characterized by a high level of extraversion (M = 32.48; SD = 7.02; sten score M = 7.21; Mo = 7), openness (M = 31.50; SD = 6.31; sten score M = 7.41; Mo = 8), low neuroticism (M = 21.62; SD = 9.33; sten score M = 4.96), predominance of present hedonistic (M = 12.55, SD = 1.46) and future time perspective (M = 11.39; SD = 2.67), and the internal health locus of control (M = 23.25, SD = 5.43). Conclusion: Group sailing can be favorable for broadly understood health and cancer recovery, but people who choose this activity have certain psychological predispositions, especially indicating high needs for stimulation. Permanent features should be taken into account when proposing various interventions for oncology patients to best suit them to their natural possibilities and preferences and, thus, make them most effective.
Differences According to Sex in the Relationship between Social Participation and Well-Being: A Network Analysis
Authors: Zhao Di , Li Guopeng , Zhou Miao , Wang Qing , Gao Yiming , Zhao Xiangyu , Zhang Xinting , Li Ping
Published: October 2022

This study aimed to explore the effects of different types of social participation on the components of well-being, as well as the differences according to sex in the relationship between social participation and well-being. This was a cross-sectional community study. Well-being was measured using the positive emotion, engagement, relationships, meaning, and accomplishment (PERMA) profile. Social participation included activities of daily life, sports and entertainment activities, and social service activities. The independent association between each type of social participation and the components of well-being was examined by using a network analysis in both males and females. Of all 1276 participants surveyed, 60% were females. The results of the network analysis showed that positive emotion–housework (0.263), positive emotion–games (0.102), engagement–housework (0.107), engagement–work (0.054), and meaning–socializing (0.085) had unique connections in males; the average predictability in the network was 0.417. For females, positive emotion–physical activity (0.102), engagement–associations or societies (0.071), relationships–physical (0.090), relationships–socializing (0.092), and relationships–volunteering activities (0.133) had significant connections; the average predictability in the network was 0.358. Different types of social participation may have different effects on the components of well-being. Furthermore, differences according to sex in the relationship between social participation and well-being should be considered when formulating interventions to improve well-being.
Children’s Attentional Processes in Outdoor and Indoor Environments: The Role of Physiological Self-Regulation
Authors: Mason Lucia , Zagni Benedetta , Bacchin Francesca , Frison Carlotta , Scrimin Sara
Published: October 2022

Previous research has documented that exposure to green spaces has the beneficial effects of attention restoration and stress reduction. This study investigated the effects of indoor (classroom) and outdoor (green school garden) environments on attentional processes in interaction with emotion and physiological self-regulation. Children in third and fourth grades (n = 42) completed a school-related emotional Stroop task assessing the effects of outdoor and indoor classroom backgrounds when facing positive and negative stimuli. Children’s attentional patterns in a task completed in both environments were also assessed. Heart rate variability was registered at rest as an index of physiological self-regulation. The results revealed that children were less distracted from negative emotional materials when presented with outdoor compared with indoor background stimuli. Greater selective attention and sustained attention were shown in the green than in the classroom environment. Moreover, sustained attention varied in relation to physiological self-regulation but only when performing the task indoor.
Volatility Spillover Effects of the US, European and Chinese Financial Markets in the Context of the Russia–Ukraine Conflict
Authors: Beraich Mohamed , Amzile Karim , Laamire Jaouad , Zirari Omar , Fadali Mohamed Amine
Published: October 2022

The present study aims to investigate the volatility spillover effects in the international financial markets before and during the Russia–Ukraine conflict. The subject of this paper is the study of the influence of the recent war between Russia and Ukraine on the transmission of volatility between the American, European and Chinese stock markets using the DY methodology. The sample period for daily data is from 1 June 2019 to 1 June 2022, excluding holidays. The volatility spillover index increased during the war period, but this increase remains insignificant compared to that recorded during the COVID-19 pandemic crisis. According to the empirical results, we also found varying levels of dependence and spillover effects between the European, American and Chinese stock indices before and during the Russia–Ukraine conflict.
Insights into the Cytochrome P450 Monooxygenase Superfamily in Osmanthus fragrans and the Role of OfCYP142 in Linalool Synthesis
Authors: Liu Jiawei , Hu Hongmin , Shen Huimin , Tian Qingyin , Ding Wenjie , Yang Xiulian , Wang Lianggui , Yue Yuanzheng
Published: October 2022

Osmanthus fragrans flowers have long been used as raw materials in food, tea, beverage, and perfume industries due to their attractive and strong fragrance. The P450 superfamily proteins have been reported to widely participate in the synthesis of plant floral volatile organic compounds (VOCs). To investigate the potential functions of P450 superfamily proteins in the fragrance synthesis of O. fragrans, we investigated the P450 superfamily genome wide. A total of 276 P450 genes were identified belonging to 40 families. The RNA-seq data suggested that many OfCYP genes were preferentially expressed in the flower or other organs, and some were also induced by multiple abiotic stresses. The expression patterns of seven flower-preferentially expressed OfCYPs during the five different flower aroma content stages were further explored using quantitative real-time PCR, showing that the CYP94C subfamily member OfCYP142 had the highest positive correlation with linalool synthesis gene OfTPS2. The transient expression of OfCYP142 in O. fragrans petals suggested that OfCYP142 can increase the content of linalool, an important VOC of the O. fragrans floral aroma, and a similar result was also obtained in flowers of OfCYP142 transgenic tobacco. Combined with RNA-seq data of the transiently transformed O. fragrans petals, we found that the biosynthesis pathway of secondary metabolites was significantly enriched, and many 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes were also upregulated. This evidence indicated that the OfCYP proteins may play critical roles in the flower development and abiotic response of O. fragrans, and that OfCYP142 can participate in linalool synthesis. This study provides valuable information about the functions of P450 genes and a valuable guide for studying further functions of OfCYPs in promoting fragrance biosynthesis of ornamental plants.
Excessive Adventitial and Perivascular Vascularisation Correlates with Vascular Inflammation and Intimal Hyperplasia
Authors: Bogdanov Leo , Shishkova Daria , Mukhamadiyarov Rinat , Velikanova Elena , Tsepokina Anna , Terekhov Alexander , Koshelev Vladislav , Kanonykina Anastasia , Shabaev Amin , Frolov Alexey , Zagorodnikov Nikita , Kutikhin Anton
Published: October 2022

Albeit multiple studies demonstrated that vasa vasorum (VV) have a crucial importance in vascular pathology, the informative markers and metrics of vascular inflammation defining the development of intimal hyperplasia (IH) have been vaguely studied. Here, we employed two rat models (balloon injury of the abdominal aorta and the same intervention optionally complemented with intravenous injections of calciprotein particles) and a clinical scenario (arterial and venous conduits for coronary artery bypass graft (CABG) surgery) to investigate the pathophysiological interconnections among VV, myeloperoxidase-positive (MPO+) clusters, and IH. We found that the amounts of VV and MPO+ clusters were strongly correlated; further, MPO+ clusters density was significantly associated with balloon-induced IH and increased at calciprotein particle-provoked endothelial dysfunction. Likewise, number and density of VV correlated with IH in bypass grafts for CABG surgery at the pre-intervention stage and were higher in venous conduits which more frequently suffered from IH as compared with arterial grafts. Collectively, our results underline the pathophysiological importance of excessive VV upon the vascular injury or at the exposure to cardiovascular risk factors, highlight MPO+ clusters as an informative marker of adventitial and perivascular inflammation, and propose another mechanistic explanation of a higher long-term patency of arterial grafts upon the CABG surgery.
Selective Recovery of Palladium (II) from Metallurgical Wastewater Using Thiadiazole-Based Chloromethyl Polystyrene-Modified Adsorbent
Authors: Zhang Xiaoguo , Chen Zhihong , Wan Zhaoneng , Liu Chali , He Renze , Xie Xiaoguang , Huang Zhangjie
Published: October 2022

Selective adsorption of palladium from metallurgical wastewater containing Pt (IV), Rh (III), Ca2+, Cu2+, Fe3+, Ni2+, Pb2+, V3+, and Ti4+ has tremendous economic and environmental benefits. In this paper, a novel thiadiazole-based chloromethyl polystyrene-modified adsorbent, viz. 2, 5-bis-polystyrene-1,3,4-thiadiazole (PS-DMTD), was synthesized using chloromethyl polystyrene as the backbone. The experimental results show that PS-DMTD can selectively separate Pd (II) from metallurgical wastewater in a one-step adsorption process. The calculated saturation adsorption capacity of PS-DMTD for Pd (II) was 176.3 mg/g at 25 °C. The separation factors of βPd (II)/Mn+ (Mn+: Pt (IV), Rh (III), Ca2+, Cu2+, Fe3+, Ni2+, Pb2+, V3+, and Ti4+) were all higher than 1 × 104. FT-IR, XPS, and single-crystal X-ray diffraction showed that the adsorption of Pd (II) to PS-DMTD was primarily through a coordination mechanism. Density functional theory (DFT) calculations revealed that the other base metal ions could not coordinate with the PS-DMTD. Pt (IV) could not be adsorbed to PS-DMTD due to its strong chlorophilicity. Furthermore, Rh (III) existed as a polyhydrate, which inhibited Rh (III) diffusion toward the positively charged absorption sites on the PS-DMTD. These results highlight that PS-DMTD has broad application prospects in the recovery of Pd (II) from metallurgical wastewater.
The Effects of High CO2 and Strigolactones on Shoot Branching and Aphid–Plant Compatibility Control in Pea
Authors: Swiegers Hendrik Willem , Karpinska Barbara , Hu Yan , Dodd Ian C. , Botha Anna-Maria , Foyer Christine H.
Published: October 2022

Elevated atmospheric CO2 concentrations (eCO2) regulate plant architecture and susceptibility to insects. We explored the mechanisms underpinning these responses in wild type (WT) peas and mutants defective in either strigolactone (SL) synthesis or signaling. All genotypes had increased shoot height and branching, dry weights and carbohydrate levels under eCO2, demonstrating that SLs are not required for shoot acclimation to eCO2. Since shoot levels of jasmonic acid (JA) and salicylic acid (SA) tended to be lower in SL signaling mutants than the WT under ambient conditions, we compared pea aphid performance on these lines under both CO2 conditions. Aphid fecundity was increased in the SL mutants compared to the WT under both ambient and eCO2 conditions. Aphid infestation significantly decreased levels of JA, isopentenyladenine, trans-zeatin and gibberellin A4 and increased ethylene precursor ACC, gibberellin A1, gibberellic acid (GA3) and SA accumulation in all lines. However, GA3 levels were increased less in the SL signaling mutants than the WT. These studies provide new insights into phytohormone responses in this specific aphid/host interaction and suggest that SLs and gibberellins are part of the network of phytohormones that participate in host susceptibility.
Integrated Bioinformatic Investigation of EXOSCs in Hepatocellular Carcinoma Followed by the Preliminary Validation of EXOSC5 in Cell Proliferation
Authors: Zhang Yujing , Yang Xinyue , Hu Yang , Huang Xin
Published: October 2022

The Exosome complex (EXOSC) is a multiprotein complex that was originally discovered as the machinery of RNA degradation. Interestingly, recent studies have reported that EXOSC family members (EXOSCs) are associated with various human diseases, including cancers. It will be interesting to investigate whether EXOSCs are related to the processes of hepatocellular carcinoma (HCC). In this study, multiple public databases and experimental validation were utilized to systemically investigate the role of EXOSCs, especially EXOSC5, in HCC. It is worth considering that the mRNA and protein levels of many EXOSCs were elevated in HCC, although there were some differences in the results from different database analyses. The over-expression of EXOSCs could predict HCC to some extent, as evidenced by the positive correlation between the elevated EXOSCs and alpha fetoprotein (AFP) levels, as well as with a high accuracy, as shown by the receiver operating characteristic curve analysis. Additionally, higher mRNA expressions of specific EXOSCs were significantly related to clinical cancer stage, shorter overall survival and disease-free survival in HCC patients. A moderate mutation rate of EXOSCs was also observed in HCC. Furthermore, a gene functional enrichment analysis indicated that EXOSCs were mainly involved in the metabolism of RNA. Moreover, we revealed that the expression of EXOSCs is remarkably related to immune cell infiltration. Finally, EXOSC5 was upregulated in HCC tissues and cell lines, promoting cell growth and proliferation via activated signal transducer and activator of transcription 3 (STAT3). The bioinformatic analyses, following verification in situ and in vitro, provided a direction for further functions and underlying mechanism of EXOSCs in HCC.
Developing Bi-Gold Compound BGC2a to Target Mitochondria for the Elimination of Cancer Cells
Authors: Cui Qingbin , Ding Wenwen , Liu Panpan , Luo Bingling , Yang Jing , Lu Wenhua , Hu Yumin , Huang Peng , Wen Shijun
Published: October 2022

Reactive oxygen species (ROS) homeostasis and mitochondrial metabolism are critical for the survival of cancer cells, including cancer stem cells (CSCs), which often cause drug resistance and cancer relapse. Auranofin is a mono-gold anti-rheumatic drug, and it has been repurposed as an anticancer agent working by the induction of both ROS increase and mitochondrial dysfunction. Hypothetically, increasing auranofin’s positive charges via incorporating more gold atoms to enhance its mitochondria-targeting capacity could enhance its anti-cancer efficacy. Hence, in this work, both mono-gold and bi-gold compounds were designed and evaluated to test our hypothesis. The results showed that bi-gold compounds generally suppressed cancer cells proliferation better than their mono-gold counterparts. The most potent compound, BGC2a, substantially inhibited the antioxidant enzyme TrxR and increased the cellular ROS. BGC2a induced cell apoptosis, which could not be reversed by the antioxidant agent vitamin C, implying that the ROS induced by TrxR inhibition might not be the decisive cause of cell death. As expected, a significant proportion of BGC2a accumulated within mitochondria, likely contributing to mitochondrial dysfunction, which was further confirmed by measuring oxygen consumption rate, mitochondrial membrane potential, and ATP production. Moreover, BGC2a inhibited colony formation and reduced stem-like side population (SP) cells of A549. Finally, the compound effectively suppressed the tumor growth of both A549 and PANC-1 xenografts. Our study showed that mitochondrial disturbance may be gold-based compounds’ major lethal factor in eradicating cancer cells, providing a new approach to developing potent gold-based anti-cancer drugs by increasing mitochondria-targeting capacity.
Proteomic Profiling of Plant and Pathogen Interaction on the Leaf Epidermis
Authors: Sidiq Yasir , Tamaoki Daisuke , Nishiuchi Takumi
Published: October 2022

The plant epidermis is the first line of plant defense against pathogen invasion, and likely contains important regulatory proteins related to the plant–pathogen interaction. This study aims to identify the candidates of these regulatory proteins expressed in the plant epidermis. We performed comparative proteomic studies to identify rapidly and locally expressed proteins in the leaf epidermis inoculated with fungal phytopathogen. The conidia solutions were dropped onto the Arabidopsis leaf surface, and then, we collected the epidermal tissues from inoculated and mock-treated leaves at 4 and 24 hpi. The label-free quantification methods showed that expressions of Arabidopsis proteins, which are related to defense signals, such as BAK1, MKK5, receptor-like protein kinases, transcription factors, and stomatal functions, were rapidly induced in the epidermal tissues of inoculated leaves. In contrast, most of them were not differentially regulated by fugal inoculation in the whole leaves. These findings clearly indicate that epidermal proteomics can monitor locally expressed proteins in inoculated areas of plant tissues. We also identified the 61 fungal proteins, including effector-like proteins specifically expressed on the Arabidopsis epidermis. Our new findings suggested that epidermal proteomics is useful for understanding the local expressions of plant and fungal proteins related to their interactions.
Acidic Shift of Optimum pH of Bovine Serum Amine Oxidase upon Immobilization onto Nanostructured Ferric Tannates
Authors: Rilievo Graziano , Cecconello Alessandro , Molinari Simone , Venerando Andrea , Rutigliano Lavinia , Govardhan Gayathri T. , Kariyawasam Dinusha H. , Arusei Ruth J. , Zennaro Lucio , Di Paolo Maria L. , Agostinelli Enzo , Vianello Fabio , Magro Massimiliano
Published: October 2022

Protein–nanoparticle hybrids represent entities characterized by emerging biological properties that can significantly differ from those of the parent components. Herein, bovine serum amine oxidase (i.e., BSAO) was immobilized onto a magnetic nanomaterial constituted of surface active maghemite nanoparticles (i.e., SAMNs, the core), surface-modified with tannic acid (i.e., TA, the shell), to produce a biologically active ternary hybrid (i.e., SAMN@TA@BSAO). In comparison with the native enzyme, the secondary structure of the immobilized BSAO responded to pH variations sensitively, resulting in a shift of its optimum activity from pH 7.2 to 5.0. Conversely, the native enzyme structure was not influenced by pH and its activity was affected at pH 5.0, i.e., in correspondence with the best performances of SAMN@TA@BSAO. Thus, an extensive NMR study was dedicated to the structure–function relationship of native BSAO, confirming that its low activity below pH 6.0 was ascribable to minimal structural modifications not detected by circular dichroism. The generation of cytotoxic products, such as aldehydes and H2O2, by the catalytic activity of SAMN@TA@BSAO on polyamine oxidation is envisaged as smart nanotherapy for tumor cells. The present study supports protein–nanoparticle conjugation as a key for the modulation of biological functions.
The Influence of Exogenous Jasmonic Acid on the Biosynthesis of Steroids and Triterpenoids in Calendula officinalis Plants and Hairy Root Culture
Authors: Rogowska Agata , Stpiczyńska Małgorzata , Pączkowski Cezary , Szakiel Anna
Published: October 2022

The interplay between steroids and triterpenoids, compounds sharing the same biosynthetic pathway but exerting distinctive functions, is an important part of the defense strategy of plants, and includes metabolic modifications triggered by stress hormones such as jasmonic acid. Two experimental models, Calendula officinalis hairy root cultures and greenhouse cultivated plants (pot plants), were applied for the investigation of the effects of exogenously applied jasmonic acid on the biosynthesis and accumulation of steroids and triterpenoids, characterized by targeted GC-MS (gas chromatography-mass spectroscopy) metabolomic profiling. Jasmonic acid elicitation strongly increased triterpenoid saponin production in hairy root cultures (up to 86-fold) and their release to the medium (up to 533-fold), whereas the effect observed in pot plants was less remarkable (two-fold enhancement of saponin biosynthesis after a single foliar application). In both models, the increase of triterpenoid biosynthesis was coupled with hampering the biomass formation and modifying the sterol content, involving stigmasterol-to-sitosterol ratio, and the proportions between ester and glycoside conjugates. The study revealed that various organs in the same plant can react differently to jasmonic acid elicitation; hairy root cultures are a useful in vitro model to track metabolic changes, and enhanced glycosylation (of both triterpenoids and sterols) seems to be important strategy in plant defense response.