bluuebunny/crossref_metadata_2025 huggingface dataset:
DFT Investigation of the Structural, Electronic, and Optical Properties of AsTi (Bi)-Phase ZnO under Pressure for Optoelectronic Applications
Authors: Adnan Muhammad , Wang Qingbo , Sohu Najamuddin , Du Shiyu , He Heming , Peng Zhenbo , Liu Zhen , Zhang Xiaohong , Bai Chengying
Published: October 2023

Pressure-induced phases of ZnO have attracted considerable attention owing to their excellent electronic and optical properties. This study provides a vital insight into the electronic structure, optical characteristics, and structural properties of the AsTi (Bi) phase of ZnO under high pressure via the DFT-based first-principles approach. The phase transformation from BN(Bk) to the Bi phase of ZnO is estimated at 16.1 GPa using local density approximation, whereas the properties are explored precisely by the hybrid functional B3LYP. The electronic structure exploration confirms that the Bi phase is an insulator with a wider direct bandgap, which expands by increasing pressure. The dielectric function evidenced that the Bi phase behaves as a dielectric in the visible region and a metallic material at 18 eV. Optical features such as the refractive index and loss function revealed the transparent nature of the Bi phase in the UV range. Moreover, the considered Bi phase is found to possess a high absorption coefficient in the ultraviolet region. This research provides strong theoretical support for the development of Bi-phase ZnO-based optoelectronic and photovoltaic devices.
Analysis of Geometric Surface Structure and Surface Layer Microhardness of Ti6Al4V Titanium Alloy after Vibratory Shot Peening
Authors: Matuszak Jakub
Published: October 2023

This article presents an analysis of the impact of vibratory shot peening on the surface roughness and physical properties of the Ti6Al4V titanium alloy surface layer after milling. The elements of machine parts and structures made of titanium alloys are often exposed to variable loads during operation. Therefore, it is advisable to apply methods that enhance functional properties and increase the durability of interacting components. Increasing the operational durability of such elements can be achieved by vibratory shot peening. Variable amplitudes A = 24; 33; 42; 51; 60 mm and times t = 1; 7; 13; 19; 25 min were applied. It has been demonstrated that it is possible to achieve a threefold reduction in the roughness parameter, Sa = 0.344 µm, compared with milling, Sa = 0.95 µm. An increase in Smr(c) areal material ratio was observed after vibratory shot peening compared with milling. It has been shown that amplitude has a greater impact on the increase in hardening of the surface layer gh compared with time. The highest rate of change in surface roughness and thickness of the hardened layer was achieved at a vibratory shot-peening time of t = 13 min. The greatest thickness of the hardened layer, exceeding 200 µm, was obtained after shot peening with an amplitude of A = 60 mm.
Surface Roughness-Induced Changes in Important Physical Features of CoFeSm Thin Films on Glass Substrates during Annealing
Authors: Fern Chi-Lon , Liu Wen-Jen , Chang Yung-Huang , Chiang Chia-Chin , Chen Yuan-Tsung , Lu Pei-Xin , Su Xuan-Ming , Lin Shih-Hung , Lin Ko-Wei
Published: October 2023

Co60Fe20Sm20 thin films were deposited onto glass substrates in a high vacuum setting. The films varied in thickness from 10 to 50 nm and underwent annealing processes at different temperatures: room temperature (RT), 100, 200, and 300 °C. Our analysis encompassed structural, magnetic, electrical, nanomechanical, adhesive, and optical properties in relation to film thickness and annealing temperature. X-ray diffraction (XRD) analysis did not reveal characteristic peaks in Co60Fe20Sm20 thin films due to insufficient growth-driving forces. Electrical measurements indicated reduced resistivity and sheet resistance with increasing film thickness and higher annealing temperatures, owing to hindered current-carrier transport resulting from the amorphous structure. Atomic force microscope (AFM) analysis showed a decrease in surface roughness with increased thickness and annealing temperature. The low-frequency alternating current magnetic susceptibility (χac) values increased with film thickness and annealing temperature. Nanoindentation analysis demonstrated reduced film hardness and Young’s modulus with thicker films. Contact angle measurements suggested a hydrophilic film. Surface energy increased with greater film thickness, particularly in annealed films, indicating a decrease in contact angle contributing to this increase. Transmittance measurements have revealed intensified absorption and reduced transmittance with thicker films. In summary, the surface roughness of CoFeSm films at different annealing temperatures significantly influenced their magnetic, electrical, adhesive, and optical properties. A smoother surface reduced the pinning effect on the domain walls, enhancing the χac value. Additionally, diminished surface roughness led to a lower contact angle and higher surface energy. Additionally, smoother surfaces exhibited higher carrier conductivity, resulting in reduced electrical resistance. The optical transparency decreased due to the smoother surface of Co60Fe20Sm20 films.
Structural Characterization and Anti-Osteoporosis Effects of a Novel Sialoglycopeptide from Tuna Eggs
Authors: Hu Shiwei , Wan Xiaofeng , Zhu Hongli , Yang Huicheng
Published: October 2023

Several sialoglycopeptides were isolated from several fish eggs and exerted anti-osteoporosis effects. However, few papers have explored sialoglycopeptide from tuna eggs (T-ES). Here, a novel T-ES was prepared through extraction with KCl solution and subsequent enzymolysis. Pure T-ES was obtained through DEAE-Sepharose ion exchange chromatography and sephacryl S-300 gel filtration chromatography. The T-ES was composed of 14.07% protein, 73.54% hexose, and 8.28% Neu5Ac, with a molecular weight of 9481 Da. The backbone carbohydrate in the T-ES was →4)-β-D-GlcN-(1→3)-α-D-GalN-(1→3)-β-D-Glc-(1→2)-α-D-Gal-(1→2)-α-D-Gal-(1→3)-α-D-Man-(1→, with two branches of β-D-GlcN-(1→ and α-D-GalN-(1→ linking at o-4 in →2,4)-α-D-Gal-(1→. Neu5Ac in the T-ES was linked to the branch of α-D-GlcN-(1→. A peptide chain, Ala-Asp-Asn-Lys-Ser*-Met-Ile that was connected to the carbohydrate chain through O-glycosylation at the –OH of serine. Furthermore, in vitro data revealed that T-ES could remarkably enhance bone density, bone biomechanical properties, and bone microstructure in SAMP mice. The T-ES elevated serum osteogenesis-related markers and reduced bone resorption-related markers in serum and urine. The present study’s results demonstrated that T-ES, a novel sialoglycopeptide, showed significant anti-osteoporosis effects, which will accelerate the utilization of T-ES as an alternative marine drug or functional food for anti-osteoporosis.
Dependence of Electrochemical Characteristics of a Biodegradable Fe-30Mn-5Si wt.% Alloy on Compressive Deformation in a Wide Temperature Range
Authors: Kadirov Pulat , Pustov Yury , Zhukova Yulia , Karavaeva Maria , Sheremetyev Vadim , Korotitskiy Andrey , Baranova Alexandra , Prokoshkin Sergey
Published: October 2023

Fe-30Mn-5Si alloy subjected to a compression test at various deformation temperatures ranging from 350 to 900 °C with a strain rate of 1 s−1 are studied. It was found that the Fe-30Mn-5Si alloy exhibits high resistance to the dynamic recrystallization process in a whole studied range of deformation temperatures. There are no differences in structure formation in the zone of action of tangential tensile stresses and peripheral and central zones of localized compressive stresses. The room-temperature X-ray diffraction study shows the presence of a single-phase state (FCC γ-austenite) after deformation temperature range from 350 to 700 °C and a two-phase state (FCC γ-austenite + HCP ε-martensite) after deformation test at 900 °C. The presence of a two-phase state provides a higher rate of biodegradation compared with a single-phase state. The changes in the biodegradation rate dependence on the structure change with an increase in the deformation temperature are explained. Favorable temperature regimes for subsequent thermomechanical processing are proposed based on the relationship between structure formation and biodegradation rate to obtain semi-products from the Fe-30Mn-5Si alloy.
Effect of Controlled Heat Treatment and Aluminum Additions on the Strengthening of Cu–Ni-Based Alloys
Authors: Rosales-Cadena Isai , Gonzalez-Rodriguez Jose Gonzalo , Diaz-Reyes Constancio , Guardian-Tapia Rene , Ruiz-Ochoa Juan Antonio , Ramirez-Arteaga America Maria , Lopez-Sesenes Roy
Published: October 2023

In this investigation, Cu–Ni alloys with different aluminum additions were synthetized under a vacuum atmosphere to reduce the material density. Annealed alloys in a He atmosphere with low aluminum concentration exhibited a coarse dendritic structure, while samples with high aluminum concentration exhibited a refined dendritic structure. Structural defects analyses have shown relatively low vacancy concentrations. Hardness evaluations indicated an increment by approximately 5 times i.e., 370 HVN, more than that for the alloyed samples compared with the as-cast and unalloyed samples. Compression tests have shown a noticeable strengthening improvement (360 MPa), mainly in samples heat treated with 10 at.% Al, while samples with 5 at.% Al showed an acceptable resistance (270 MPa) as well. In general, the sample with 10 at.% Al presented the best performance to be considered as potential structural material.
Transit Time Theory for a Droplet Passing through a Slit in Pressure-Driven Low Reynolds Number Flows
Authors: Borbas Spencer W. , Shen Kevin , Ji Catherine , Viallat Annie , Helfer Emmanuèle , Peng Zhangli
Published: October 2023

Soft objects squeezing through small apertures are crucial for many in vivo and in vitro processes. Red blood cell transit time through splenic inter-endothelial slits (IESs) plays a crucial role in blood filtration and disease progression, while droplet velocity through constrictions in microfluidic devices is important for effective manipulation and separation processes. As these transit phenomena are not well understood, we sought to establish analytical and numerical solutions of viscous droplet transit through a rectangular slit. This study extends from our former theory of a circular pore because a rectangular slit is more realistic in many physiological and engineering applications. Here, we derived the ordinary differential equations (ODEs) of a droplet passing through a slit by combining planar Poiseuille flow, the Young–Laplace equations, and modifying them to consider the lubrication layer between the droplet and the slit wall. Compared to the pore case, we used the Roscoe solution instead of the Sampson one to account for the flow entering and exiting a rectangular slit. When the surface tension and lubrication layer were negligible, we derived the closed-form solutions of transit time. When the surface tension and lubrication layer were finite, the ODEs were solved numerically to study the impact of various parameters on the transit time. With our solutions, we identified the impact of prescribed pressure drop, slit dimensions, and droplet parameters such as surface tension, viscosity, and volume on transit time. In addition, we also considered the effect of pressure drop and surface tension near critical values. For this study, critical surface tension for a given pressure drop describes the threshold droplet surface tension that prevents transit, and critical pressure for a given surface tension describes the threshold pressure drop that prevents transit. Our solutions demonstrate that there is a linear relationship between pressure and the reciprocal of the transit time (referred to as inverse transit time), as well as a linear relationship between viscosity and transit time. Additionally, when the droplet size increases with respect to the slit dimensions, there is a corresponding increase in transit time. Most notably, we emphasize the initial antagonistic effect of surface tension which resists droplet passage but at the same time decreases the lubrication layer, thus facilitating passage. Our results provide quantitative calculations for understanding cells passing through slit-like constrictions and designing droplet microfluidic experiments.
Trajectories and Forces in Four-Electrode Chambers Operated in Object-Shift, Dielectrophoresis and Field-Cage Modes—Considerations from the System’s Point of View
Authors: Gimsa Jan , Radai Michal M.
Published: October 2023

In two previous papers, we calculated the dielectrophoresis (DEP) force and corresponding trajectories of high- and low-conductance 200-µm 2D spheres in a square 1 × 1-mm chamber with plane-versus-pointed, plane-versus-plane and pointed-versus-pointed electrode configurations by applying the law of maximum entropy production (LMEP) to the system. Here, we complete these considerations for configurations with four-pointed electrodes centered on the chamber edges. The four electrodes were operated in either object-shift mode (two adjacent electrodes opposite the other two adjacent electrodes), DEP mode (one electrode versus the other three electrodes), or field-cage mode (two electrodes on opposite edges versus the two electrodes on the other two opposite edges). As in previous work, we have assumed DC properties for the object and the external media for simplicity. Nevertheless, every possible polarization ratio of the two media can be modeled this way. The trajectories of the spherical centers and the corresponding DEP forces were calculated from the gradients of the system’s total energy dissipation, described by numerically-derived conductance fields. In each of the three drive modes, very high attractive and repulsive forces were found in front of pointed electrodes for the high and low-conductance spheres, respectively. The conductance fields predict bifurcation points, watersheds, and trajectories with multiple endpoints. The high and low-conductance spheres usually follow similar trajectories, albeit with reversed orientations. In DEP drive mode, the four-point electrode chamber provides a similar area for DEP measurements as the classical plane-versus-pointed electrode chamber.
Plant-Associated Representatives of the Bacillus cereus Group Are a Rich Source of Antimicrobial Compounds
Authors: Vater Joachim , Tam Le Thi Thanh , Jähne Jennifer , Herfort Stefanie , Blumenscheit Christian , Schneider Andy , Luong Pham Thi , Thao Le Thi Phuong , Blom Jochen , Klee Silke R. , Schweder Thomas , Lasch Peter , Borriss Rainer
Published: October 2023

Seventeen bacterial strains able to suppress plant pathogens have been isolated from healthy Vietnamese crop plants and taxonomically assigned as members of the Bacillus cereus group. In order to prove their potential as biocontrol agents, we perform a comprehensive analysis that included the whole-genome sequencing of selected strains and the mining for genes and gene clusters involved in the synthesis of endo- and exotoxins and secondary metabolites, such as antimicrobial peptides (AMPs). Kurstakin, thumolycin, and other AMPs were detected and characterized by different mass spectrometric methods, such as MALDI-TOF-MS and LIFT-MALDI-TOF/TOF fragment analysis. Based on their whole-genome sequences, the plant-associated isolates were assigned to the following species and subspecies: B. cereus subsp. cereus (6), B. cereus subsp. bombysepticus (5), Bacillus tropicus (2), and Bacillus pacificus. These three isolates represent novel genomospecies. Genes encoding entomopathogenic crystal and vegetative proteins were detected in B. cereus subsp. bombysepticus TK1. The in vitro assays revealed that many plant-associated isolates enhanced plant growth and suppressed plant pathogens. Our findings indicate that the plant-associated representatives of the B. cereus group are a rich source of putative antimicrobial compounds with potential in sustainable agriculture. However, the presence of virulence genes might restrict their application as biologicals in agriculture.
Structure–Activity Relationship Target Prediction Studies of Clindamycin Derivatives with Broad-Spectrum Bacteriostatic Antibacterial Properties
Authors: Jia Yiduo , Zhang Yinmeng , Zhu Hong
Published: October 2023

This study investigated the potential of clindamycin derivatives with broad-spectrum antibacterial properties. The main goal was to identify new antibacterial targets to lay the foundation for developing novel antimicrobial agents. This research used molecular docking and dynamics simulations to explore how clindamycin derivatives could combat bacterial resistance and widen their antibacterial capabilities. Three different clindamycin derivatives were studied against 300 target proteins. Among these, 26 proteins were found to be common targets for all three derivatives. After further screening through molecular docking and dynamics simulations, four specific protein targets were identified. Notably, one of these targets, cell division protein FtsZ, was found to be primarily located in the cyto and cyto_nucl compartments. These findings suggest that clindamycin derivatives have the potential to address bacterial resistance and broaden their antibacterial effectiveness through these identified protein targets.
Application of Nanoparticles in Cancer Treatment: A Concise Review
Authors: Sell Mariana , Lopes Ana Rita , Escudeiro Maria , Esteves Bruno , Monteiro Ana R. , Trindade Tito , Cruz-Lopes Luísa
Published: October 2023

Timely diagnosis and appropriate antitumoral treatments remain of utmost importance, since cancer remains a leading cause of death worldwide. Within this context, nanotechnology offers specific benefits in terms of cancer therapy by reducing its adverse effects and guiding drugs to selectively target cancer cells. In this comprehensive review, we have summarized the most relevant novel outcomes in the range of 2010–2023, covering the design and application of nanosystems for cancer therapy. We have established the general requirements for nanoparticles to be used in drug delivery and strategies for their uptake in tumor microenvironment and vasculature, including the reticuloendothelial system uptake and surface functionalization with protein corona. After a brief review of the classes of nanovectors, we have covered different classes of nanoparticles used in cancer therapies. First, the advances in the encapsulation of drugs (such as paclitaxel and fisetin) into nanoliposomes and nanoemulsions are described, as well as their relevance in current clinical trials. Then, polymeric nanoparticles are presented, namely the ones comprising poly lactic-co-glycolic acid, polyethylene glycol (and PEG dilemma) and dendrimers. The relevance of quantum dots in bioimaging is also covered, namely the systems with zinc sulfide and indium phosphide. Afterwards, we have reviewed gold nanoparticles (spheres and anisotropic) and their application in plasmon-induced photothermal therapy. The clinical relevance of iron oxide nanoparticles, such as magnetite and maghemite, has been analyzed in different fields, namely for magnetic resonance imaging, immunotherapy, hyperthermia, and drug delivery. Lastly, we have covered the recent advances in the systems using carbon nanomaterials, namely graphene oxide, carbon nanotubes, fullerenes, and carbon dots. Finally, we have compared the strategies of passive and active targeting of nanoparticles and their relevance in cancer theranostics. This review aims to be a (nano)mark on the ongoing journey towards realizing the remarkable potential of different nanoparticles in the realm of cancer therapeutics.
Associations between Changes in Food Acquisition Behaviors, Dietary Intake, and Bodyweight during the COVID-19 Pandemic among Low-Income Parents in California
Authors: Woodward-Lopez Gail , Esaryk Erin , Rauzon Suzanne , Hewawitharana Sridharshi C. , Thompson Hannah R. , Cordon Ingrid , Whetstone Lauren
Published: October 2023

COVID-19 disrupted food access, potentially increasing nutritional risk and health inequities. This study aimed to describe and assess associations between changes in food/meal acquisition behaviors and relative changes in dietary intake and bodyweight from before to during the pandemic. Low-income parents (n = 1090) reported these changes by online survey in April–August 2021. Associations were assessed by multinomial logistic regression. Compared to those with no change, those who decreased supermarket shopping had greater odds of decreased fruit and vegetable (FV; OR[95%CI] = 2.4[1.4–4.1]) and increased salty snack intakes (OR[95%CI] = 1.7[1.0–2.8]). Those who decreased farmer’s market shopping had greater odds of decreased FV intake (OR[95%CI] = 1.8[1.0–3.1]), increased bodyweight (OR[95%CI] = 1.7[1.1–2.6]), and increased SSB (OR[95%CI] = 1.9[1.1–3.2]) and sweets intakes (OR[95%CI] = 1.8[1.1–2.9]). Those who increased online food ordering had greater odds of increased sweets (OR[95%CI] = 1.7[1.1–2.8]), salty snacks (OR[95%CI] = 1.9[1.2–3.2]), and fast food (OR[95%CI] = 2.0[1.2–3.5]) intakes and bodyweight (OR[95%CI] = 1.8[1.1–2.9]). Those who increased healthy meal preparation had greater odds of increased FV intake (OR[95%CI] = 4.0[2.5–6.5]), decreased SSB (OR[95%CI] = 3.7[2.3–6.0]), sweets (OR[95%CI] = 2.7[1.6–4.4]), salty snacks (OR[95%CI] = 3.0[1.8–5]) and fast food intakes (OR[95%CI] = 2.8[1.7–4.6]) and bodyweight (OR[95%CI] = 2.2[1.2–4.0]). Interventions to address the potentially negative impacts of online food/meal shopping and support healthy home cooking may be needed to improve nutrition-related outcomes and reduce health disparities in the aftermath of the current pandemic and during future emergencies requiring similar restrictions.
Longitudinal Associations of Dietary Fiber Intake with Glycated Hemoglobin and Estimated Insulin Sensitivity in Adults with and without Type 1 Diabetes
Authors: Basu Arpita , Hooyman Andrew , Richardson Leigh Ann , Alman Amy C. , Snell-Bergeon Janet K.
Published: October 2023

Dietary fiber, an essential bioactive compound in plant-based diets, is of public health concern based on habitual low intakes in the general population. Not much data are available on how habitual dietary fiber is associated with glycemic control in type 1 diabetes (T1D) as well as in prediabetes and normoglycemic adults. To address this gap, we conducted a six-year longitudinal analysis of an original cohort in adults with and without T1D (n = 1255; T1D: n = 563; non-diabetes mellitus (non-DM): n = 692). Dietary data were collected from a validated food frequency questionnaire, biochemical measures were obtained after an overnight fast, and anthropometric measurements were collected at baseline as well as after three and six years for the follow-up study. Glycated hemoglobin (HbA1c) and estimated insulin sensitivity (eIS) were the main outcomes examined. In adjusted analyses, dietary fiber intake was inversely associated with HbA1c in a minimally adjusted model, but it was positively associated with eIS in a model involving all relevant covariates in non-DM adults. These associations were not significant in the T1D group. Furthermore, when examined by HbA1c cut-offs for glycemic control, an inverse association with dietary fiber was only observed in adults with prediabetes (all p < 0.05). At a six-year mean (±SD) dietary fiber intake of 17.4 ± 8.8 g for non-DM and 17.0 ± 8.2 g for the T1D group, protective associations against poor glycemic control were observed in those without diabetes and in prediabetes.
The Role of Human Milk Oligosaccharides in Myelination, Socio-Emotional and Language Development: Observational Data from Breast-Fed Infants in the United States of America
Authors: Rajhans Purva , Mainardi Fabio , Austin Sean , Sprenger Norbert , Deoni Sean , Hauser Jonas , Schneider Nora
Published: October 2023

Infancy is a critical period for neurodevelopment, which includes myelination, synaptogenesis, synaptic pruning, and the development of motor, social-emotional, and cognitive functions. Human milk provides essential nutrients to the infant’s developing brain, especially during the first postnatal months. Human milk oligosaccharides (HMOs) are a major component of human milk, and there is growing evidence of the association of individual HMOs with cognitive development in early life. However, to our knowledge, no study has explained these associations with a mechanism of action. Here, we investigated possible mediating associations between HMOs in human milk, brain myelination (measured via myelin water fraction), and measures of motor, language (collected via the Bayley Scales of Infant and Toddler Development (Bayley-III)), and socioemotional development (collected via the Ages and Stages Questionnaire: Social-Emotional Version (ASQ-SE)) in healthy term-born breast-fed infants. The results revealed an association between 6′Sialyllactose and social skills that was mediated by myelination. Furthermore, associations of fucosylated HMOs with language outcomes were observed that were not mediated by myelination. These observations indicate the roles of specific HMOs in neurodevelopment and associated functional outcomes, such as social-emotional function and language development.
Effects of Healthy Lifestyles on Chronic Diseases: Diet, Sleep and Exercise
Authors: Oster Henrik , Chaves Inês
Published: October 2023

Among the many factors affecting general health and resilience to disease, lifestyle is at the same time the most controllable and the most influential factor [...]
Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress
Authors: Letenneur Vivien , Monnoye Magali , Philippe Catherine , Holowacz Sophie , Rabot Sylvie , Lepage Patricia , Jacouton Elsa , Naudon Laurent
Published: October 2023

The effect of supplementation with Lactobacillus strains to prevent the consequences of chronic stress on anxiety in mouse strains sensitive to stress and the consequences on gut microbiota have been relatively unexplored. Thus, we administered a Lacticaseibacillus casei LA205 and Lacticaseibacillus paracasei LA903 mix to male BALB/cByJrj mice two weeks before and during 21-day chronic restraint stress (CRS) (non-stressed/solvent (NS-PBS), non-stressed/probiotics (NS-Probio), CRS/solvent (S-PBS), CRS/probiotics (S-Probio)). CRS resulted in lower body weight and coat state alteration, which were attenuated by the probiotic mix. S-Probio mice showed less stress-associated anxiety-like behaviours than their NS counterpart, while no difference was seen in PBS mice. Serum corticosterone levels were significantly higher in the S-Probio group than in other groups. In the hippocampus, mRNA expression of dopamine and serotonin transporters was lower in S-Probio than in S-PBS mice. Few differences in bacterial genera proportions were detected, with a lower relative abundance of Alistipes in S-Probio vs. S-PBS. CRS was accompanied by a decrease in the proportion of caecal acetate in S-PBS mice vs. NS-PBS, but not in the intervention groups. These data show that the probiotic mix could contribute to better coping with chronic stress, although the precise bacterial mechanism is still under investigation.
Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides
Authors: Cardoso Ennya Rafaella Neves , Carvalho Stephani Félix , Dias Sarah Alves , Santos Rayane Almeida , Tavares Mariana Avelar , Neves Lucianne Cardoso , Paula Warley Vieira de Freitas , Pádua Gracielle Teles , de Lima Nicolas Jalowitzki , Paludo Raquel Loren dos Reis , Silva Isabela Santos , Bittencourt Raphaela Bueno Mendes , dos Santos Gabriel Cândido , Nascimento Flavia Giovana de Jesus , de Paula Luiza Gabriella Ferreira , Dantas-Torres Filipe , Monteiro Caio Marcio De Oliveira , Krawczak Felipe da Silva
Published: October 2023

Amblyomma sculptum is a relevant tick species from a One Health perspective, playing an important role as a vector of Rickettsia rickettsii, the main agent of spotted fever rickettsiosis in Brazil. In this study, we evaluated the susceptibility of two A. sculptum populations from Goiás state (midwestern Brazil) to different acaricides. The first tick population (GYN strain) originated from an experimental farm, where the ticks are annually exposed to acaricides. The second (PNE strain) was collected in a national park (Emas National Park), where the ticks had not been exposed to acaricides. Immersion tests were conducted with 21-day-old laboratory-reared larvae and nymphs originating from adult ticks collected in the areas mentioned above. The chosen acaricides were two synthetic pyrethroids (cypermethrin and deltamethrin), one organophosphate (chlorfenvinphos), one formamidine (amitraz), and two combinations of pyrethroids and organophosphates (cypermethrin, chlorpyrifos and citronellal; cypermethrin, fenthion and chlorpyrifos). Mortality data were used to determine the lethal concentration (LC) values at which 50%, 90%, and 99% of the ticks died (LC50, LC90, and LC99, respectively), and resistance ratios (RR) were calculated based on the LC values. The RR revealed differences between the acaricide-exposed (GYN) and unexposed (PNE) tick strains. The PNE strain larvae and nymphs were susceptible to all the tested acaricides. The GYN strain larvae were tolerant to cypermethrin, whereas the nymphs were tolerant to deltamethrin, chlorfenvinphos, and the combination of cypermethrin, chlorpyrifos, and citronellal (2 < RR ≤ 10). The GYN strain nymphs were resistant to amitraz (RR > 10). This is the first report of A. sculptum nymphs with resistance to amitraz and tolerance to deltamethrin, chlorfenvinphos, and the combination of cypermethrin, chlorpyrifos, and citronellal.
Drug-Resistant Aspergillus spp.: A Literature Review of Its Resistance Mechanisms and Its Prevalence in Europe
Authors: De Francesco Maria Antonia
Published: October 2023

Infections due to the Aspergillus species constitute an important challenge for human health. Invasive aspergillosis represents a life-threatening disease, mostly in patients with immune defects. Drugs used for fungal infections comprise amphotericin B, triazoles, and echinocandins. However, in the last decade, an increased emergence of azole-resistant Aspergillus strains has been reported, principally belonging to Aspergillus fumigatus species. Therefore, both the early diagnosis of aspergillosis and its epidemiological surveillance are very important to establish the correct antifungal therapy and to ensure a successful patient outcome. In this paper, a literature review is performed to analyze the prevalence of Aspergillus antifungal resistance in European countries. Amphotericin B resistance is observed in 2.6% and 10.8% of Aspergillus fumigatus isolates in Denmark and Greece, respectively. A prevalence of 84% of amphotericin B-resistant Aspergillus flavus isolates is reported in France, followed by 49.4%, 35.1%, 21.7%, and 20% in Spain, Portugal, Greece, and amphotericin B resistance of Aspergillus niger isolates is observed in Greece and Belgium with a prevalence of 75% and 12.8%, respectively. The prevalence of triazole resistance of Aspergillus fumigatus isolates, the most studied mold obtained from the included studies, is 0.3% in Austria, 1% in Greece, 1.2% in Switzerland, 2.1% in France, 3.9% in Portugal, 4.9% in Italy, 5.3% in Germany, 6.1% in Denmark, 7.4% in Spain, 8.3% in Belgium, 11% in the Netherlands, and 13.2% in the United Kingdom. The mechanism of resistance is mainly driven by the TR34/L98H mutation. In Europe, no in vivo resistance is reported for echinocandins. Future studies are needed to implement the knowledge on the spread of drug-resistant Aspergillus spp. with the aim of defining optimal treatment strategies.
Topical Formulations Based on Ursolic Acid-Loaded Nanoemulgel with Potential Application in Psoriasis Treatment
Authors: Miastkowska Małgorzata , Kulawik-Pióro Agnieszka , Lasoń Elwira , Śliwa Karolina , Malinowska Magdalena Anna , Sikora Elżbieta , Kantyka Tomasz , Bielecka Ewa , Maksylewicz Anna , Klimaszewska Emilia , Ogorzałek Marta , Tabaszewska Małgorzata , Skoczylas Łukasz , Nowak Krzysztof
Published: October 2023

Psoriasis is a chronic disorder that causes a rash with itchy, scaly patches. It affects nearly 2–5% of the worldwide population and has a negative effect on patient quality of life. A variety of therapeutic approaches, e.g., glucocorticoid topical therapy, have shown limited efficacy with systemic adverse reactions. Therefore, novel therapeutic agents and physicochemical formulations are in constant need and should be obtained and tested in terms of effectiveness and minimization of side effects. For that reason, the aim of our study was to design and obtain various hybrid systems, nanoemulgel–macroemulsion and nanoemulgel–oleogel (bigel), as vehicles for ursolic acid (UA) and to verify their potential as topical formulations used in psoriasis treatment. Obtained topical formulations were characterized by conducting morphological, rheological, texture, and stability analysis. To determine the safety and effectiveness of the prepared ursolic acid carriers, in vitro studies on human keratinocyte cell-like HaCaT cells were performed with cytotoxicity analysis for individual components and each formulation. Moreover, a kinetic study of ursolic acid release from the obtained systems was conducted. All of the studied UA-loaded systems were well tolerated by keratinocyte cells and had suitable pH values and stability over time. The obtained formulations exhibit an apparent viscosity, ensuring the appropriate time of contact with the skin, ease of spreading, soft consistency, and adherence to the skin, which was confirmed by texture tests. The release of ursolic acid from each of the formulations is followed by a slow, controlled release according to the Korsmeyer–Peppas and Higuchi models. The elaborated systems could be considered suitable vehicles to deliver triterpene to psoriatic skin.
Predicting the Potential Geographical Distribution of Rhodiola L. in China under Climate Change Scenarios
Authors: Yang Meilin , Sun Lingxiao , Yu Yang , Zhang Haiyan , Malik Ireneusz , Wistuba Małgorzata , Yu Ruide
Published: October 2023

Rhodiola L. has high nutritional and medicinal value. Little is known about the properties of its habitat distribution and the important eco-environmental factors shaping its suitability. Rhodiola coccinea (Royle) Boriss., Rhodiola gelida Schrenk, Rhodiola kirilowii (Regel) Maxim., and Rhodiola quadrifida (Pall.) Fisch. et Mey., which are National Grade II Protected Plants, were selected for this research. Based on high-resolution environmental data for the past, current, and future climate scenarios, we modeled the suitable habitat for four species by MaxEnt, evaluated the importance of environmental factors in shaping their distribution, and identified distribution shifts under climate change scenarios. The results indicate that the growth distribution of R. coccinea, R. kirilowii, and R. quadrifida is most affected by bio10 (mean temperature of warmest quarter), bio3 (isothermality), and bio12 (annual precipitation), whereas that of R. gelida is most affected by bio8 (mean temperature of wettest quarter), bio13 (precipitation of wettest month), and bio16 (precipitation of wettest quarter). Under the current climate scenario, R. coccinea and R. quadrifida are primarily distributed in Tibet, eastern Qinghai, Sichuan, northern Yunnan, and southern Gansu in China, and according to the 2070 climate scenario, the suitable habitats for both species are expected to expand. On the other hand, the suitable habitats for R. gelida and R. kirilowii, which are primarily concentrated in southwestern Xinjiang, Tibet, eastern Qinghai, Sichuan, northern Yunnan, and southern Gansu in China, are projected to decrease under the 2070 climate scenario. Given these results, the four species included in our study urgently need to be subjected to targeted observation management to ensure the renewal of Rhodiola communities. In particular, R. gelida and R. kirilowii should be given more attention. This study provides a useful reference with valuable insights for developing effective management and conservation strategies for these four nationally protected plant species.
GhDof1.7, a Dof Transcription Factor, Plays Positive Regulatory Role under Salinity Stress in Upland Cotton
Authors: Li Yi , Tian Miaomiao , Feng Zhen , Zhang Jingjing , Lu Jianhua , Fu Xiaokang , Ma Liang , Wei Hengling , Wang Hantao
Published: October 2023

Salt stress is a major abiotic stressor that can severely limit plant growth, distribution, and crop yield. DNA-binding with one finger (Dof) is a plant-specific transcription factor that plays a crucial role in plant growth, development, and stress response. In this study, the function of a Dof transcription factor, GhDof1.7, was investigated in upland cotton. The GhDof1.7 gene has a coding sequence length of 759 base pairs, encoding 252 amino acids, and is mainly expressed in roots, stems, leaves, and inflorescences. Salt and abscisic acid (ABA) treatments significantly induced the expression of GhDof1.7. The presence of GhDof1.7 in Arabidopsis may have resulted in potential improvements in salt tolerance, as suggested by a decrease in H2O2 content and an increase in catalase (CAT) and superoxide dismutase (SOD) activities. The GhDof1.7 protein was found to interact with GhCAR4 (C2-domain ABA-related 4), and the silencing of either GhDof1.7 or GhCAR4 resulted in reduced salt tolerance in cotton plants. These findings demonstrate that GhDof1.7 plays a crucial role in improving the salt tolerance of upland cotton and provide insight into the regulation of abiotic stress response by Dof transcription factors.
Potential and Design Parameters of Polyvinylidene Fluoride in Gear Applications
Authors: Muratović Enis , Muminović Adil , Delić Muamer , Pervan Nedim , Muminović Adis J. , Šarić Isad
Published: October 2023

(1) Background: With the ever-increasing number of polymer materials and limited data on polymer gear calculations, designers are often required to perform extensive experimental testing in order to establish reliable operational data for specific gear applications. This research investigates the potential of a Polyvinyldene fluoride (PVDF) polymer material in gear applications, considering various loading conditions and different types of gear transmission configurations, including both self-mated mesh and steel/PVDF mesh. (2) Methods: PVDF gear samples were tested on a specially designed test rig that enables active torque control and temperature monitoring in order to obtain the necessary design parameters and failure modes. Each test for certain load conditions was repeated five times, and to fully investigate the potential of PVDF gear samples, comparative testing was performed for Polyoxymethylene (POM) gear. (3) Results: Tribological compatibility, tooth load capacity, and lifespan assessment, along with the types of failure, which, for some configurations, include several types of failures, such as wear and melting, were determined. Temperature monitoring data were used to estimate the coefficient of friction at the tooth contact of analyzed gear pairs, while optical methods were used to determine a wear coefficient. (4) Conclusions: The tribological compatibility of polymer gear pairs needs to be established in order to design a gear pair for a specific application. PVDF gear samples mated with steel gear showed similar lifespan properties compared to POM samples. Temperature monitoring and optical methods serve as a basis for the determination of the design parameters. PVDF is an appropriate material to use in gear applications, considering its comparable properties with POM. The particular significance of this research is reflected in the establishment of the design parameters of PVDF gear, as well as in the analysis of the potential of the PVDF material in gear applications, which gives exceptional significance to the current knowledge on polymer gears, considering that the PVDF material has not previously been analyzed in gear applications.
Ternary Blends from Biological Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), Poly(propylene carbonate) and Poly(vinyl acetate) with Balanced Properties
Authors: Jin Yujie , Han Changyu , Li Yi , Cheng Hongda , Li Dongdong , Wang Huan
Published: October 2023

Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) has gained significant attention because of its biodegradability and sustainability. However, its expanded application in some fields is limited by the brittleness and low melt viscoelasticity. In this work, poly(vinyl acetate) (PVAc) was introduced into PHBH/poly(propylene carbonate) (PPC) blends via melt compounding with the aim of obtaining a good balance of properties. Dynamic mechanical analysis results suggested that PPC and PHBH were immiscible. PVAc was miscible with both a PHBH matrix and PPC phase, while it showed better miscibility with PHBH than with PPC. Therefore, PVAc was selectively localized in a PHBH matrix, reducing interfacial tension and refining dispersed phase morphology. The crystallization rate of PHBH slowed down, and the degree of crystallinity decreased with the introduction of PPC and PVAc. Moreover, the PVAc phase significantly improved the melt viscoelasticity of ternary blends. The most interesting result was that the remarkable enhancement of toughness for PHBH/PPC blends was obtained by adding PVAc without sacrificing the strength markedly. Compared with the PHBH/PPC blend, the elongation at the break and yield strength of the PHBH/PPC/10PVAc blend increased by 1145% and 7.9%, respectively. The combination of high melt viscoelasticity, toughness and strength is important for the promotion of the practical application of biological PHBH.
Peeling Force Required for the Detachment of Non-Woven Plastic Tissue from the Surface of Mortar Prisms
Authors: Bahij Sifatullah , Omary Safiullah , Belhaj Essia , Steiner Vincent , Feugeas Francoise
Published: October 2023

The purpose of this experimental paper is to examine the adhesion properties between non-woven plastic sheets and cement mortar. Specifically, the effect of w/c ratio and quantity of superplasticizer on the peeling force required for the detachment of tissue from the surface of prisms was studied in detail. Therefore, two types of mortar mixtures were prepared: (1) mixtures without superplasticizer with three different w/c ratios of 0.45, 0.50, and 0.55, and (2) mixtures with reduced amounts of water and three various percentages of superplasticizer of 0.0%, 1.11%, and 2.17% (by weight of cement). For this purpose, bond tests with a special setup, interferometry and microscopic analyses, and mechanical tests were performed. The results highlight that non-woven sheets had strong adhesion to cement mortar without using any adhesive materials. However, the peeling force improved by 15.78% as the w/c ratio increased from 0.50 to 0.55. Conversely, this force declined by 24.50% as the w/c ratio decreased from 0.50 to 0.45. In addition, the peeling force decreased by 20.62% as the w/c ratio decreased from 0.50 to 0.45 and 1.11% superplasticizer was added to the mixtures. This property decreased further by 38.29% as the w/c ratio lowered to 0.40, and the amount of superplasticizer increased to 2.17%. The interferometry and microscopic analyses clearly demonstrate that the adhesion between tissue and mortar is largely related to the surface texture, amount of cement paste, and quantity of residual fibers on the surfaces of samples. It indicates that mortar samples with higher w/c ratios had a smoother surface, and providing more contact area for microfilaments, which resulted in thicker layers of remaining fibers compared to the specimens with a lower w/c ratio. Even though there was not much difference in the surface texture of specimens with superplasticizer and lower w/c ratios, because of their similar workability. Still, thicker layers of microfilaments remained on the surface of specimens containing a lower amount of superplasticizer, which resulted in strong adhesion between sheet and cement mortar.
Advances of Nano-Enabled ZnFe2O4 Based-Gas Sensors for VOC Detection and Their Potential Applications: A Review
Authors: Nemufulwi Murendeni I. , Swart Hendrik C. , Mhlongo Gugu H.
Published: October 2023

The demand for reliable gas sensing technologies in chemical, manufacturing, environmental, and occupational sites has increased in the last few decades following the global volatile gas sensor market, which is expected to grow further beyond 2025. Currently, several types of sensors have been employed for applications in different fields. Optical sensors are widely implemented in mining and environmental monitoring. Conventional food testing methods are utilized for the detection of any chemical or microbial agent in the food industry. Although robust and sensitive, most sensing technologies are expensive, labor-intensive, and necessitate the use of time-consuming gas sampling pretreatment steps, and these issues impede the achievement of quick, simple detection, portable, and cost-effective gas monitoring. For this reason, researchers around the world are investigating the possibility of using gas sensors as a promising technology that has the potential to alleviate industrial safety concerns. As a highly sensitive semiconducting metal oxide, gas sensors based on ZnFe2O4 have the potential to ensure environmental and occupational safety in real time. This review introduces and highlights recent developments in ZnFe2O4 gas sensors for application in different fields. The challenges limiting the wide application of the ZnFe2O4 sensor are outlined. Furthermore, this review discusses the common strategies adopted to improve the sensing properties of ZnFe2O4 for gas detection. Finally, future perspectives on further improvements of ZnFe2O4 sensing properties are discussed, and integration of ZnFe2O4 sensors into electronic noses to tackle the selectivity issue and how they can feature on the Internet of Things is outlined.
Religious Buildings in Early al-Andalus: Origins, Consolidation and Prevalence in Urban Contexts
Authors: González-Gutiérrez Carmen
Published: October 2023

The arrival of Islam to the Iberian Peninsula at the beginning of the 8th c. brought important changes to the urbanism of cities which contributed to turn the previous late-antique realities into medieval Islamic settlements. Among all the transformations that took place, the introduction of mosques and the reconfiguration of cities’ religioscapes is one of the most relevant. The processes through which the earliest mosques were first inserted in urban landscapes in al-Andalus are unclear, since so far there are no remains that can be undoubtedly dated before the Umayyad period. From that moment on, and alongside the Umayyad organization of the Andalusi state, the founding of mosques becomes clearer and traceable, and their urban, religious and political roles more evident. This contribution seeks to identify how and why mosques appeared in the Iberian Peninsula, how they (re)configured religious spaces in cities, and how they contributed to consolidate their significance through specific written and architectural narratives. This topic will be explored also seeking for parallels and connections in the Bilād al-Shām region.