bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Omic Approaches to Breast Cancer Metabolic Phenotyping: Applications in Diagnosis, Prognosis, and the Development of Novel Treatments
Authors: Gómez-Cebrián Nuria , Domingo-Ortí Inés , Poveda José Luis , Vicent María J. , Puchades-Carrasco Leonor , Pineda-Lucena Antonio
Published: September 2021

Breast cancer (BC) is characterized by high disease heterogeneity and represents the most frequently diagnosed cancer among women worldwide. Complex and subtype-specific gene expression alterations participate in disease development and progression, with BC cells known to rewire their cellular metabolism to survive, proliferate, and invade. Hence, as an emerging cancer hallmark, metabolic reprogramming holds great promise for cancer diagnosis, prognosis, and treatment. Multi-omics approaches (the combined analysis of various types of omics data) offer opportunities to advance our understanding of the molecular changes underlying metabolic rewiring in complex diseases such as BC. Recent studies focusing on the combined analysis of genomics, epigenomics, transcriptomics, proteomics, and/or metabolomics in different BC subtypes have provided novel insights into the specificities of metabolic rewiring and the vulnerabilities that may guide therapeutic development and improve patient outcomes. This review summarizes the findings of multi-omics studies focused on the characterization of the specific metabolic phenotypes of BC and discusses how they may improve clinical BC diagnosis, subtyping, and treatment.
Oncologic Imaging of the Lymphatic System: Current Perspective with Multi-Modality Imaging and New Horizon
Authors: Elshikh Mohamed , Moawad Ahmed W , Salem Usama , Klimkowski Sergio P , Hassan Talaat , Rao Korivi Brinda , Jensen Corey T , Javadi Sanaz , Elsayes Khaled M
Published: September 2021

The lymphatic system is an anatomically complex vascular network that is responsible for interstitial fluid homeostasis, transport of large interstitial particles and cells, immunity, and lipid absorption in the gastrointestinal tract. This network of specially adapted vessels and lymphoid tissue provides a major pathway for metastatic spread. Many malignancies produce vascular endothelial factors that induce tumoral and peritumoral lymphangiogenesis, increasing the likelihood for lymphatic spread. Radiologic evaluation for disease staging is the cornerstone of oncologic patient treatment and management. Multiple imaging modalities are available to access both local and distant metastasis. In this manuscript, we review the anatomy, physiology, and imaging of the lymphatic system.
Role of Diagnostic Imaging in Chronic Recurrent Multifocal Osteomyelitis (CRMO) in Children: An Observational Study
Authors: Kopeć Michał , Brąszewska Magdalena , Jarosz Mariusz , Dylewska Katarzyna , Kurylak Andrzej
Published: September 2021

The aim of this single-center observational study was to analyze the applicability of various imaging studies to the diagnosis and further evaluation of patients with chronic recurrent multifocal osteomyelitis (CRMO). The analysis included the data of 10 patients with CRMO treated between 2016 and 2021. The mean ages of the patients at the first manifestation of CRMO and ultimate diagnosis were 10 years and 7 months and 11 years and 10 months, respectively. Conventional radiography demonstrated focal loss of bone density in only 30% of the patients. Computed tomography showed disseminated foci with non-homogeneous osteolytic/osteosclerotic structure, with a massive loss of cortical layer and strong periosteal reaction. On magnetic resonance imaging (MRI), most patients presented with multifocal hypodense areas on T1-weighted images, with the enhancement of signal on T-weighted and STIR sequences. The duration of follow-up varied between 3 months and 3 years. In 40% of the patients, both clinical symptoms and the abnormalities seen on MRI resolved completely, whereas another 50% showed partial regression of clinical and radiological manifestations. MRI findings, co-existing with characteristic clinical manifestations, play a pivotal role in establishing the ultimate diagnosis of CRMO. MRI can also be used to monitor the outcomes of treatment in CRMO patients.
The Effects of Exercise Difficulty and Time-of-Day on the Perception of the Task and Soccer Performance in Child Soccer Players
Authors: Masmoudi Liwa , Gharbi Adnene , H’Mida Cyrine , Trabelsi Khaled , Boukhris Omar , Chtourou Hamdi , Bouzid Mohamed Amine , Clark Cain C. T. , Souissi Nizar , Rosemann Thomas , Knechtle Beat
Published: September 2021

In soccer, accurate kicking skills are important determinants of successful performance. A successful kick must meet several criteria, including speed, accuracy, and timing. In fact, players who are able to kick the ball more accurately under various difficulties, such as time pressure, space constraints, the opponent’s pressure, and the distance between the kicking point and the goal, have a clear advantage during soccer games. The aim of the present study was to investigate the effect of exercise difficulty and time-of-day on perceived task difficulty and kicking performance. Accordingly, 32 boys (age: 11 ± 0.7 years; height: 1.45 ± 0.07 m; body-mass: 38.9 ± 7.8 kg) performed shooting accuracy tests under two difficulty levels (distance (long-distance (LD) vs. short-distance (SD)) and time pressure (Without-time-pressure (WTP) vs. With-time-pressure (TP)) at 08:00 h and 17:00 h. Absolute-error, variable-error, and constant-error were evaluated during the kicking tasks, in addition to ball velocity and shooting quality. Moreover, rating-of-perceived-exertion score (RPE), feeling-scale (FS), and perceived difficulty were completed immediately at the end of each test. The results showed that shooting quality was not affected by the time-of-day, but it was better in WTP vs. TP (p < 0.05), and in SD vs. LD (p < 0.05), respectively. Higher values for FS and lower values for RPE were observed in the morning compared to the afternoon (p < 0.05) and in WTP vs. TP (p < 0.05). In conclusion, specific soccer skills of boys were not time-of-day dependent, but they may be associated with time pressure and task difficulty.
Pyrrolylquinoline-BF2 and BPh2 BODIPY-Type Analogues: Synthesis, Structural Analysis and Photophysical Properties
Authors: Alsimaree Abdulrahman A. , Alatawi Omar M. , Waddell Paul G. , Day David P. , Alsenani Nawaf I. , Knight Julian G.
Published: September 2021

Two new pyrrolylquinoline-substituted heteroaromatic-containing compounds bearing a central boron bridge have been prepared by a short, high-yielding sequence consisting of Suzuki-coupling of 8-bromoquinoline and N-Boc 2-pyrroleboronic acid, thermolytic tert-butyloxycarbonyl deprotection, and subsequent boron chelation (either using boron trifluoride or triphenylborane). Both derivatives display longer wavelength absorption maxima (λabsmax) than a previously reported indolopyridine-BPh2 analogue, in agreement with the smaller HOMO-LUMO energy gap predicted by DFT quantum chemical calculations. Both of the pyrrolylquinoline-boron chelates display weak emission (quantum yields 0.3–0.9%) and the BPh2 complex displays a very broad, long-wavelength emission (λemmax = 715 nm, MeCN), which may be due to dimer emission and results in a large pseudo-Stokes’ shift (7753 cm−1) for this compound.
Comparative Approaches in Vertebrate Cartilage Histogenesis and Regulation: Insights from Lampreys and Hagfishes
Authors: Root Zachary D. , Gould Claire , Brewer Margaux , Jandzik David , Medeiros Daniel M.
Published: September 2021

Jawed vertebrates (gnathostomes) have been the dominant lineage of deuterostomes for nearly three hundred fifty million years. Only a few lineages of jawless vertebrates remain in comparison. Composed of lampreys and hagfishes (cyclostomes), these jawless survivors are important systems for understanding the evolution of vertebrates. One focus of cyclostome research has been head skeleton development, as its evolution has been a driver of vertebrate morphological diversification. Recent work has identified hyaline-like cartilage in the oral cirri of the invertebrate chordate amphioxus, making cyclostomes critical for understanding the stepwise acquisition of vertebrate chondroid tissues. Our knowledge of cyclostome skeletogenesis, however, has lagged behind gnathostomes due to the difficulty of manipulating lamprey and hagfish embryos. In this review, we discuss and compare the regulation and histogenesis of cyclostome and gnathostome skeletal tissues. We also survey differences in skeletal morphology that we see amongst cyclostomes, as few elements can be confidently homologized between them. A recurring theme is the heterogeneity of skeletal morphology amongst living vertebrates, despite conserved genetic regulation. Based on these comparisons, we suggest a model through which these mesenchymal connective tissues acquired distinct histologies and that histological flexibility in cartilage existed in the last common ancestor of modern vertebrates.
State of the Art: An Update on Adult Burn Resuscitation
Authors: Causbie Jacqueline M. , Sattler Lauren A. , Basel Anthony P. , Britton Garrett W. , Cancio Leopoldo C.
Published: September 2021

Treatment of patients with severe burn injuries is complex, relying on attentive fluid resuscitation, successful management of concomitant injuries, prompt wound assessment and closure, early rehabilitation, and compassionate psychosocial care. The goal of fluid resuscitation is to maintain organ perfusion at the lowest possible physiologic cost. This requires careful, hourly titration of the infusion rate to meet individual patient needs, and no more; the risks of over-resuscitation, such as compartment syndromes, are numerous and life-threatening. Recognizing runaway resuscitations and understanding how to employ adjuncts to crystalloid resuscitation are paramount to preventing morbidity and mortality. This article provides an update on fluid resuscitation techniques in burn patients, to include choosing the initial fluid infusion rate, using alternate endpoints of resuscitation, and responding to the difficult resuscitation.
Suppression of Ganoderma boninense Using Benzoic Acid: Impact on Cellular Ultrastructure and Anatomical Changes in Oil Palm Wood
Authors: Fernanda Rozi , Siddiqui Yasmeen , Ganapathy Daarshini , Ahmad Khairulmazmi , Surendran Arthy
Published: September 2021

Basal stem rot (BSR) caused by a wood degrading fungus, Ganoderma boninense, is the major constraint in palm oil production. It degrades the wood components and causes palms to collapse, leading to heavy losses. Inefficacy in controlling this disease could be caused by the lack of understanding in how the pathogen establishes itself on the host concerning wood decay stages. This study aimed to understand and determine the role of benzoic acid on the suppression of G. boninense and production of ligninolytic enzymes responsible for wood decay. Further, the alteration in wood component structure due to G. boninense infection and its prevention were studied. Benzoic acid treatment resulted in more than 80% of inhibition in G. boninense growth. SEM and HR-TEM analysis confirmed the antifungal activity of benzoic acid by disruption of mycelial morphology and cellular ultrastructure. Moreover, the membrane permeability assay recorded enhanced cell mortality in benzoic acid treated mycelium. The degradation of oil palm woodblock caused 58.86 % wood dry weight loss at day 120. In contrast, reduction in dry weight loss (58.82%) was recorded in woodblock treated with concentrations of benzoic acid of 5 mM and above. It is concluded that the use of benzoic acid could inhibit or delay pathogen establishment in oil palm wood, leading to the sustainable management of BSR disease. Further, glasshouse and field trials are required to prove the consistency in current findings which may contribute to reduced land expansion to create new disease-free land for oil palm planting.
Prediction of the Carbon Content of Six Tree Species from Visible-Near-Infrared Spectroscopy
Authors: Meng Yongbin , Zhang Yuanyuan , Li Chunxu , Zhao Jinghan , Wang Zichun , Wang Chen , Li Yaoxiang
Published: September 2021

This study aimed to measure the carbon content of tree species rapidly and accurately using visible and near-infrared (Vis-NIR) spectroscopy coupled with chemometric methods. Currently, the carbon content of trees used for calculating the carbon storage of forest trees in the study of carbon sequestration is obtained by two methods. One involves measuring carbon content in the laboratory (K2CrO7-H2SO4 oxidation method or elemental analyzer), and another involves directly using the IPCC (Intergovernmental Panel on Climate Change) default carbon content of 0.45 or 0.5. The former method is destructive, time-consuming, and expensive, while the latter is subjective. However, Vis-NIR detection technology can avoid these shortcomings and rapidly determine carbon content. In this study, 96 increment core samples were collected from six tree species in the Heilongjiang province of China for analysis. The spectral data were preprocessed using seven methods, including extended multiplicative scatter correction (EMSC), first derivative (1D), second derivative (2D), baseline correction, de-trend, orthogonal signal correction (OSC), and normalization to eliminate baseline drifting and noise, as well as to enhance the model quality. Linear models were established from the spectra using partial least squares regression (PLS). At the same time, we also compared the effects of full-spectrum and reduced spectrum on the model’s performance. The results showed that the spectral data processed by 1D with the full spectrum could obtain a better prediction model. The 1D method yielded the highest R2c of 0.92, an RMSEC (root-mean-square error of calibration) of 0.0056, an R2p of 0.99, an RMSEP (root-mean-square error of prediction) of 0.0020, and the highest RPD (residual prediction deviation) value of 8.9. The results demonstrate the feasibility of Vis-NIR spectroscopy coupled with chemometric methods in determining the carbon content of tree species as a simple, rapid, and non-destructive method.
Citric Acid Influences the Dynamics of the Fermentation Quality, Protease Activity and Microbial Community of Mulberry Leaf Silage
Authors: Chen Dekui , Zhou Wei , Guo Xiang , Zheng Mingyang , Chen Xiaoyang , Zhang Qing
Published: September 2021

Mulberry (Morus alba) leaves has performed well as a high-quality protein supplement for livestock and enriches the edible resources of livestock. However, the harvest of mulberry leaves is seasonal and occurs mainly during the rainy season in southeast China; therefore, humid and sultry weather causes serious losses of mulberry leaf biomass, which pose a challenge for the preservation of mulberry leaves. In this study, we used the silage fermentation method to preserve mulberry leaves and investigated the effects of citric acid on the silage quality of mulberry leaves. Mulberry leaves were ensiled with or without 1% citric acid and 2% citric acid. The chemical composition, protein fraction and microbial community of mulberry leaf silages were analyzed. The results showed that the silage treated with citric acid had a higher dry matter recovery and lactic acid content and a lower acetic acid content, non-protein nitrogen content and ammonia-N content; citric acid also inhibited the activities of carboxypeptidase and aminopeptidase. Moreover, citric acid increased Lactobacillus abundance in silages and decreased the abundance of undesired microorganisms, such as Enterobacter. In summary, the addition of citric acid improved the fermentation quality of mulberry leaf silages, with 2% citric acid being more effective than 1% citric acid.
Anti-Osteoporotic Activity of Pueraria lobata Fermented with Lactobacillus paracasei JS1 by Regulation of Osteoblast Differentiation and Protection against Bone Loss in Ovariectomized Mice
Authors: Kim Seon Yu , Lee Hee-Ju , Kim Taehyun , Lee Yeong-Geun , Kwon Jeong Eun , Kang Se Chan
Published: September 2021

Osteoporosis is the most common bone disease associated with low bone mineral density. It is the process of bone loss and is most commonly caused by decreased estrogen production in women, particularly after menopause. Pueraria lobata, which contains various metabolites, especially isoflavone, is widely known as regulator for bone mineral contents. In this study, the effects of the P. lobata extract (PE) with or without fermentation with Lactobacillus paracasei JS1 (FPE) on osteoporosis were investigated in vitro and in vivo. The effects of PE and FPE on human osteoblastic MG63 cells, RAW 264.7 cells, and ovariectomized (OVX)-induced model mice were analyzed at various ratios. We found that FPE increased calcium deposition and inhibited bone resorption by in vitro assay. Furthermore, treatment with PE and FPE has significantly restored destroyed trabecular bone in the OVX-induced bone loss mouse model. Overall, FPE demonstrated bioactivity to prevent bone loss by decreasing bone turnover.
Catastrophic Bushfires, Indigenous Fire Knowledge and Reframing Science in Southeast Australia
Authors: Fletcher Michael-Shawn , Romano Anthony , Connor Simon , Mariani Michela , Maezumi Shira Yoshi
Published: September 2021

The catastrophic 2019/2020 Black Summer bushfires were the worst fire season in the recorded history of Southeast Australia. These bushfires were one of several recent global conflagrations across landscapes that are homelands of Indigenous peoples, homelands that were invaded and colonised by European nations over recent centuries. The subsequent suppression and cessation of Indigenous landscape management has had profound social and environmental impacts. The Black Summer bushfires have brought Indigenous cultural burning practices to the forefront as a potential management tool for mitigating climate-driven catastrophic bushfires in Australia. Here, we highlight new research that clearly demonstrates that Indigenous fire management in Southeast Australia produced radically different landscapes and fire regimes than what is presently considered “natural”. We highlight some barriers to the return of Indigenous fire management to Southeast Australian landscapes. We argue that to adequately address the potential for Indigenous fire management to inform policy and practice in managing Southeast Australian forest landscapes, scientific approaches must be decolonized and shift from post-hoc engagement with Indigenous people and perspectives to one of collaboration between Indigenous communities and scientists.
A Novel Splicing Variant of COL2A1 in a Fetus with Achondrogenesis Type II: Interpretation of Pathogenicity of In-Frame Deletions
Authors: Bruni Valentina , Spoleti Cristina Barbara , La Barbera Andrea , Dattilo Vincenzo , Colao Emma , Votino Carmela , Bellacchio Emanuele , Perrotti Nicola , Giglio Sabrina , Iuliano Rodolfo
Published: September 2021

Achondrogenesis type II (ACG2) is a lethal skeletal dysplasia caused by dominant pathogenic variants in COL2A1. Most of the variants found in patients with ACG2 affect the glycine residue included in the Gly-X-Y tripeptide repeat that characterizes the type II collagen helix. In this study, we reported a case of a novel splicing variant of COL2A1 in a fetus with ACG2. An NGS analysis of fetal DNA revealed a heterozygous variant c.1267-2_1269del located in intron 20/exon 21. The variant occurred de novo since it was not detected in DNA from the blood samples of parents. We generated an appropriate minigene construct to study the effect of the variant detected. The minigene expression resulted in the synthesis of a COL2A1 messenger RNA lacking exon 21, which generated a predicted in-frame deleted protein. Usually, in-frame deletion variants of COL2A1 cause a phenotype such as Kniest dysplasia, which is milder than ACG2. Therefore, we propose that the size and position of an in-frame deletion in COL2A1 may be relevant in determining the phenotype of skeletal dysplasia.
Agenesis of the Corpus Callosum with Facial Dysmorphism and Intellectual Disability in Sibs Associated with Compound Heterozygous KDM5B Variants
Authors: Lebon Sébastien , Quinodoz Mathieu , Peter Virginie G. , Gengler Carole , Blanchard Gaëlle , Cina Viviane , Campos-Xavier Belinda , Rivolta Carlo , Superti-Furga Andrea
Published: September 2021

We studied a family in which the first-born child, a girl, had developmental delay, facial dysmorphism, and agenesis of the corpus callosum (ACC). The subsequent pregnancy was interrupted as the fetus was found to be also affected by ACC. Both cases were heterozygous for two KDM5B variants predicting p (Ala635Thr) and p (Ser1155AlafsTer4) that were shown to be in trans. KDM5B variants have been previously associated with moderate to severe developmental delay/intellectual disability (DD/ID), autism spectrum disorders (ASD), and dysmorphism in a few individuals, but the pathogenetic mechanisms are not clear yet as patients with both monoallelic and biallelic variants have been observed. Interestingly, one individual has previously been reported with ACC and severe ID in association with biallelic KDM5B variants. Together with the observations in this family, this suggests that agenesis of the corpus callosum may be part of the phenotypic spectrum associated with KDM5B variants and that the KDM5B gene should be included in gene panels to clarify the etiology of ACC both in the prenatal and postnatal setting.
Occurrence and Health Risk Assessment of Aflatoxins through Intake of Eastern Herbal Medicines Collected from Four Districts of Southern Punjab—Pakistan
Authors: Javed Aqib , Naeem Iqra , Benkerroum Noreddine , Riaz Muhammad , Akhtar Saeed , Ismail Amir , Sajid Muhammad , Tayyab Khan Muhammad , Ismail Zubair
Published: September 2021

Eastern herbal medicines (HMs) are plant-derived naturally occurring substances with minimum or no industrial processing that have long been used in traditional medicine. Aflatoxins are frequent contaminants of plants. Therefore, these mycotoxins are likely to contaminate HMs and pose a health risk to individuals using them on a regular basis as preventive or curative treatments of various diseases. The present study aimed to determine aflatoxin levels in the most popular Pakistani HM formulations and to assess the health risk associated with the intake of aflatoxins. A total of 400 samples of HM formulations collected from four districts of Punjab were analyzed for the quantification of aflatoxins, out of which 52.5% were found to be contaminated. The average daily dose (ADD) of AFB1 and AFs through the intake of HM formulations ranged between 0.00483 and 0.118 ng/kg bw/day and between 0.00579 and 1.714 ng/kg bw/day, respectively. The margin of exposure (MOE) and population cancer risk ranged from 99.49 to 29378.8 and from 0.00011 to 0.0325 liver cancer cases/105 individuals/year (0.0075–2.455 liver cancer cases/105 individuals/75 years), respectively. Despite the low exposure to aflatoxins from HM formulations in the four studied Punjab (Pakistan) districts, the frequent contamination of the analyzed samples suggests that official measures should be considered to manage the associated risk.
Media Representations of Science during the First Wave of the COVID-19 Pandemic: A Qualitative Analysis of News and Social Media on the Island of Ireland
Authors: O’Connor Cliodhna , O’Connell Nicola , Burke Emma , Nolan Ann , Dempster Martin , Graham Christopher D. , Nicolson Gail , Barry Joseph , Scally Gabriel , Crowley Philip , Zgaga Lina , Mather Luke , Darker Catherine D.
Published: September 2021

COVID-19 is arguably the most critical science communication challenge of a generation, yet comes in the wake of a purported populist turn against scientific expertise in western societies. This study advances understanding of science–society relations during the COVID-19 pandemic by analysing how science was represented in news and social media coverage of COVID-19 on the island of Ireland. Thematic analysis was performed on a dataset comprising 952 news articles and 603 tweets published between 1 January and 31 May 2020. Three themes characterised the range of meanings attached to science: ‘Defining science: Its subjects, practice and process’, ‘Relating to science: Between veneration and suspicion’ and ‘Using science: As solution, policy and rhetoric’. The analysis suggested that the COVID-19 pandemic represented a platform to highlight the value, philosophy, process and day-to-day activity of scientific research. However, the study also identified risks the pandemic might pose to science communication, including feeding public alienation by disparaging lay understandings, reinforcing stereotypical images of scientists, and amplifying the politicisation of scientific statements.
Sleep Bruxism in Children: Etiology, Diagnosis, and Treatment—A Literature Review
Authors: Bulanda Sylwia , Ilczuk-Rypuła Danuta , Nitecka-Buchta Aleksandra , Nowak Zuzanna , Baron Stefan , Postek-Stefańska Lidia
Published: September 2021

(1) Background: Bruxism, a condition characterized by grinding and involuntary clenching of the teeth, is a risk factor for the development of masticatory dysfunction. It can occur together with sleep disturbances and may be associated with abnormal body movements, breathing difficulties, increased muscle activity, and heart rate disturbances. This disorder is becoming an important dental concern in children. (2) Methods: A literature review was carried out based on a search in PubMed and Google Scholar databases for articles on bruxism in children, published between 2014 and 2021. The etiology, diagnosis, and treatment of bruxism in children were of particular interest in the study. (3) Results: A total of 40 articles were included in the review. The analyzed studies indicated that the prevalence rates of bruxism in children vary widely from 13% to 49%. The etiology of bruxism is complex and incomprehensible, and the main diagnostic criteria for this condition in children are subjective observation, clinical history, and clinical examination. The recommended therapy for sleep bruxism in children is physiotherapy and psychotherapy. (4) Conclusions: Dentists and primary care physicians should correctly diagnose bruxism in children, educate parents, prevent potential consequences for oral health, and identify possible comorbidities. Appropriate clinical guidelines for the treatment and prophylaxis of bruxism in children should also be developed.
Genome of Helicobacter pylori and Serotype of HPV Detected in Oropharyngeal and Laryngeal Cancer and Chronic Inflammation Patients
Authors: Astl Jaromír , Holy Richard , Maute Eva , Rotnágl Jan , Kalfeřt David , Drnková Barbora , Younus Temoore , Pavlík Emil
Published: September 2021

Objective: Oropharyngeal/laryngeal carcinoma are common cancers of the upper aerodigestive system. Human papillomavirus (HPV) is described as the most frequent in the cancer of unknown primary. The presence of Helicobacter pylori (HP) in the oral cavity is discussed in some papers. The aim of study: To analyze the incidence of HPV and HP in oropharyngeal/laryngeal cancer persons versus persons with chronic tonsillar inflammation and healthy persons. Methods: The samples were taken in three groups: (1) tissue of oropharynx/larynx cancer (103 specimens); (2) tissue of palatine tonsils (85 specimens); and (3) healthy control group (50 specimens). We analyzed the presence of HP (PCR) and HPV genomic DNA (Sacace HPV High-Risk Screen Real-TM Quant) in the samples. Results: HP was detected in 86 samples (83.5%) and high-risk HPV in 62 samples (60.2%). We found a very high incidence of HP. In the cancer group, HP was detected in 82.5% cases and HPV positivity in 57.8%. In total, 7.2% of the cancer patients were negative for HP and HPV together. In turn, 53.6% of the cancer patients were positive for HP and HPV together. Four cases (4.2%) were positive for HPV only. VacA positivity was detected in 82 (79.6%) of the cancer cases and VacA negativity in 21 (20.4%) if the cancer cases. The incidence of HP in chronic inflammation (n = 85) was 65 cases (76.5%) and the incidence of HPV was 38 cases (44.7%). VacA positivity was detected in 59 (69.4%) of the chronic inflammation cases and VacA negativity was found in 26 (30.6%) of the chronic inflammation cases. Regarding the control group, we found HP positivity in 5 cases (11.1%) and HPV positivity in 19 cases (42.2%). There was VacA positivity in 6 cases (50.0%) of the control group. Statistically significantly lower prevalence of HP (p < 0.001) and HPV (p = 0.006) was found in the control group. Conclusions: We suggest that the palatine tonsils are colonized by HP. In our study, HP was present in oropharyngeal cancer in more cases in comparison with HPV infection. The presence of VacA from HP can have an influence on the human epithelial and immune cells’ regulation ways. Our results do not support idea that the CagA-positive HP is a primary carcinogen in oropharyngeal area.
Effects of Relaxed Lockdown on Pediatric ER Visits during SARS-CoV-2 Pandemic in Italy
Authors: Matera Luigi , Nenna Raffaella , Ardenti Morini Francesca , Banderali Giuseppe , Calvani Mauro , Calvi Matteo , Cozzi Giorgio , Falsaperla Raffaele , Guidi Roberto , Kantar Ahmad , Lanari Marcello , Lubrano Riccardo , Messini Beatrice , Niccoli Antonio Augusto , Tipo Vincenzo , Midulla Fabio ,   on behalf of the CICERO Group
Published: September 2021

Previously, we demonstrated an 81% reduction in pediatric Emergency Room (ER) visits in Italy during the strict lockdown due to the SARS-CoV-2 pandemic. Since May 2020, lockdown measures were relaxed until 6 November 2020, when a strict lockdown was patchily reintroduced. Our aim was to evaluate the impact of the relaxed lockdown on pediatric ER visits in Italy. We performed a retrospective multicenter study involving 14 Italian pediatric ERs. We compared total ER visits from 24 September 2020 to 6 November 2020 with those during the corresponding timeframe in 2019. We evaluated 17 ER specific diagnoses grouped in air communicable and non-air communicable diseases. We recognized four different triage categories: white, green, yellow and red. In 2020 total ER visits were reduced by 51% compared to 2019 (16,088 vs. 32,568, respectively). The decrease in air communicable diseases was significantly higher if compared to non-air communicable diseases (−64% vs. −42%, respectively). ER visits in each triage category decreased in 2020 compared to 2019, but in percentage, white and red codes remained stable, while yellow codes slightly increased and green codes slightly decreased. Our results suggest that preventive measures drastically reduced the circulation of air communicable diseases even during the reopening of social activities but to a lesser extent with regard to the strict lockdown period (March–May 2020).
“Getting Used to It, but Still Unwelcome”: A Grounded Theory Study of Physical Identity Development in Later Life
Authors: Jin Bora , Roumell Elizabeth A.
Published: September 2021

Given the global trends toward an aging society and the increased desire for healthy aging in late life, this study examines older adults’ perceptions of aging and their physical identity through their engagement in physical activities. Adopting a grounded theory, we interviewed 15 individuals aged 65 years and older, who were involved in physical activities on a regular basis. This study provided a final model depicting (a) divergent and convergent modes of strategies and socioemotional aspects of physical identity development in later life and (b) different strategies employed between younger-old versus older-old age groups and between participants who have underlying health conditions and those who do not. These findings add a contextual explanation of identity development in later life and stress the recurring process of physical identity development.
Spatiotemporal Trends, Sources and Ecological Risks of Heavy Metals in the Surface Sediments of Weitou Bay, China
Authors: Chen Qiuming , Huang Faming , Cai Anran
Published: September 2021

Heavy metals are extremely harmful materials to marine ecosystems and human health. To determine the anthropogenic contributions and ecological risks in Weitou Bay, China, the spatiotemporal variations in the concentrations of heavy metals in surface sediment were investigated during spring 2008 and 2017. The results indicated that high concentrations of pollutants were generally located near the river mouths and along the coast of industrial areas. Principal component analysis indicated that heavy metal contents were mainly affected by industrial waste drainage, urban development, natural weathering and erosion, and interactions between organic matter and sulfides. The potential ecological risk assessment demonstrated that, in 2008, 82% of the sampling sites were at low risk, while 18% were at moderate risk. The situation had deteriorated slightly by 2017, with 73%, 18%, and 9% of stations in Waytou Bay at low, moderate, and very high risk, respectively. Cd was the most harmful metal, followed by Hg. These two elements accounted for more than 80% of the potential ecological risk index (RI) value. The present work analyzed the source of heavy metals, identified the major pollution elements and high risk areas, and provides guidance for pollution control and ecological restoration in Weitou Bay.
Ctenolepisma longicaudatum Escherich (1905) Became a Common Pest in Europe: Case Studies from Czechia and the United Kingdom
Authors: Kulma Martin , Bubová Terezie , Davies Matthew Paul , Boiocchi Federica , Patoka Jiří
Published: September 2021

Synanthropic invasive silverfish, Ctenolepisma longicaudatum, has been recently reported to cause nuisance in the indoor environment in many European countries. To get more details on the species distribution, the species occurrence was monitored by the authors in the countries where establishment of C. longicaudatum has been revealed in the last years. In Czechia, 20 findings from 14 municipalities in eight regions were recorded within the last three years. In the United Kingdom, 49 cases, including the first occurrence in Scotland, were recorded. Five cases were recorded for the Republic of Ireland. Domestic settings were the main habitat in the study countries (50.0% for the Czechia and Ireland and 36.8% for the United Kingdom). Regarding C. longicaudatum control, the standard silverfish strategy fails, and the use of insecticidal baits complemented by dust insecticides was suggested as the most promising approach. To reveal presence of C. longicaudatum in Europe, the search of literature, social platforms and databases on invasive species was conducted. According to these sources, the species is known from majority of European countries, when the high increase of records in recent decade was detected.
A Diamond-Based Dose-per-Pulse X-ray Detector for Radiation Therapy
Authors: Pettinato Sara , Girolami Marco , Olivieri Riccardo , Stravato Antonella , Caruso Cristina , Salvatori Stefano
Published: September 2021

One of the goals of modern dynamic radiotherapy treatments is to deliver high-dose values in the shortest irradiation time possible. In such a context, fast X-ray detectors and reliable front-end readout electronics for beam diagnostics are crucial to meet the necessary quality assurance requirements of care plans. This work describes a diamond-based detection system able to acquire and process the dose delivered by every single pulse sourced by a linear accelerator (LINAC) generating 6-MV X-ray beams. The proposed system is able to measure the intensity of X-ray pulses in a limited integration period around each pulse, thus reducing the inaccuracy induced by unnecessarily long acquisition times. Detector sensitivity under 6-MV X-photons in the 0.1–10 Gy dose range was measured to be 302.2 nC/Gy at a bias voltage of 10 V. Pulse-by-pulse measurements returned a charge-per-pulse value of 84.68 pC, in excellent agreement with the value estimated (but not directly measured) with a commercial electrometer operating in a continuous integration mode. Significantly, by intrinsically holding the acquired signal, the proposed system enables signal processing even in the millisecond period between two consecutive pulses, thus allowing for effective real-time dose-per-pulse monitoring.
Surface and Subsurface Analysis of Stainless Steel and Titanium Alloys Exposed to Ultrasonic Pulsating Water Jet
Authors: Poloprudský Jakub , Chlupová Alice , Šulák Ivo , Kruml Tomáš , Hloch Sergej
Published: September 2021

This article deals with the effect of periodically acting liquid droplets on the polished surfaces of AISI 316L stainless steel and Ti6Al4V titanium alloy. These materials were exposed to a pulsating water jet produced using an ultrasonic sonotrode with an oscillation frequency of 21 kHz placed in a pressure chamber. The only variable in the experiments was the time for which the materials were exposed to water droplets, i.e., the number of impingements; the other parameters were kept constant. We chose a low number of impingements to study the incubation stages of the deformation caused by the pulsating water jet. The surfaces of the specimens were studied using (1) confocal microscopy for characterizing the surface profile induced by the water jet, (2) scanning electron microscopy for detailed surface observation, and (3) transmission electron microscopy for detecting the changes in the near-surface microstructure. The surface described by the height of the primary profile of the surface increased with the number of impingements, and was substantially more intense in the austenitic steel than in the Ti alloy. Irregular surface depressions, slip lines, and short cracks were observed in the Ti alloy, whereas pronounced straight slip bands formed in the austenitic steel. The dislocation density near the surface was measured quantitatively, reaching high values of the order of 1014 m−2 in the austenitic steel and even higher values (up to 3 × 1015 m−2) in the Ti alloy. The origins of the mentioned surface features differed in the two materials: an intense dislocation slip on parallel slip planes for the Ti alloy and mechanical twinning combined with dislocation slip for the austenitic steel.
High Pinning Force Values of a Fe(Se, Te) Single Crystal Presenting a Second Magnetization Peak Phenomenon
Authors: Galluzzi Armando , Buchkov Krastyo , Tomov Vihren , Nazarova Elena , Leo Antonio , Grimaldi Gaia , Polichetti Massimiliano
Published: September 2021

The magnetization M of an Fe(Se, Te) single crystal has been measured as a function of temperature T and dc magnetic field H. The sample properties have been analyzed in the case of a magnetic field parallel to its largest face H||ab. From the M(T) measurement, the Tc of the sample and a magnetic background have been revealed. The superconducting hysteresis loops M(H) were between 2.5 K and 15 K showing a tilt due to the presence of a magnetic signal measured at T > Tc. From the M(H) curves, the critical current density Jc(H) has been extracted at different temperatures showing the presence of a second magnetization peak phenomenon. By extracting and fitting the Jc(T) curves at different fields, a pinning regime crossover has been identified and shown to be responsible for the origin of the second magnetization peak phenomenon. Then, the different kinds of pinning centers of the sample were investigated by means of Dew-Hughes analysis, showing that the pinning mechanism in the sample can be described in the framework of the collective pinning theory. Finally, the values of the pinning force density have been calculated at different temperatures and compared with the literature in order to understand if the sample is promising for high-current and high-power applications.
Initiation and Suppression of Crack Propagation during Magnesium Alloy Rolling
Authors: Tian Jing , Shi Quan-Xin , Meng Li-Xin , Deng Jia-Fei , Liang Wei , Ma Jin-Yao
Published: September 2021

The conventional rolling of magnesium alloy with a single pass and large reduction will cause severe edge cracking. The sheet without cracks can be achieved by limited width rolling. The microstructure evolution of the sheet with cracks after conventional rolling and the sheet without cracks after limited width rolling is explored, and an effective mechanism for solving edge cracks is proposed. Conventional rolling can fully develop twin evolution due to high deformation, and three stages of twinning evolution can be observed and the secondary twins easily become the nucleation points of micro cracks, resulting in a large number of cracks propagating along the twin lamellae. Cracks terminate at dislocation accumulation because the accumulation of a large number of dislocations can hinder propagation. Dislocation shearing of twins to eliminate the high localization caused by twins and induce the tensile twins to weaken the basal surface texture provides an effective plastic deformation mechanism of crack inhibition, which is useful for expanding the engineering application of magnesium alloy rolled sheets.
Oxides for Rectenna Technology
Authors: Mitrovic Ivona Z. , Almalki Saeed , Tekin Serdar B. , Sedghi Naser , Chalker Paul R. , Hall Stephen
Published: September 2021

The quest to harvest untapped renewable infrared energy sources has led to significant research effort in design, fabrication and optimization of a self-biased rectenna that can operate without external bias voltage. At the heart of its design is the engineering of a high-frequency rectifier that can convert terahertz and infrared alternating current (AC) signals to usable direct current (DC). The Metal Insulator Metal (MIM) diode has been considered as one of the ideal candidates for the rectenna system. Its unparalleled ability to have a high response time is due to the fast, femtosecond tunneling process that governs current transport. This paper presents an overview of single, double and triple insulator MIM diodes that have been fabricated so far, in particular focusing on reviewing key figures of merit, such as zero-bias responsivity (β0), zero-bias dynamic resistance (R0) and asymmetry. The two major oxide contenders for MInM diodes have been NiO and Al2O3, in combination with HfO2, Ta2O5, Nb2O5, ZnO and TiO2. The latter oxide has also been used in combination with Co3O4 and TiOx. The most advanced rectennas based on MI2M diodes have shown that optimal (β0 and R0) can be achieved by carefully tailoring fabrication processes to control oxide stoichiometry and thicknesses to sub-nanometer accuracy.
Molecular Characterizations of Anisotropic Mixed-Norm Hardy Spaces and Their Applications
Authors: Liu Jun , Huang Long , Yue Chenlong
Published: September 2021

Let p→∈(0,∞)n be an exponent vector and A be a general expansive matrix on Rn. Let HAp→(Rn) be the anisotropic mixed-norm Hardy spaces associated with A defined via the non-tangential grand maximal function. In this article, using the known atomic characterization of HAp→(Rn), the authors characterize this Hardy space via molecules with the best possible known decay. As an application, the authors establish a criterion on the boundedness of linear operators from HAp→(Rn) to itself, which is used to explore the boundedness of anisotropic Calderón–Zygmund operators on HAp→(Rn). In addition, the boundedness of anisotropic Calderón–Zygmund operators from HAp→(Rn) to the mixed-norm Lebesgue space Lp→(Rn) is also presented. The obtained boundedness of these operators positively answers a question mentioned by Cleanthous et al. All of these results are new, even for isotropic mixed-norm Hardy spaces on Rn.
Is Mathematics Required for Cooking? An Interdisciplinary Approach to Integrating Computational Thinking in a Culinary and Restaurant Management Course
Authors: Chen Yen-Cheng , Tsui Pei-Ling , Lee Ching-Sung
Published: September 2021

In recent years, with the flourishing of the catering economy and the trends in computer technology, restaurant operators have increasingly relied on employees with computational and information skills. Breaking through the traditional teaching method of mere lecturing, the study conducts a teaching demonstration by integrating the computational thinking concept and a Microsoft Excel computational system on the school’s E-learning platform into the teaching of a Culinary and Restaurant Management course. A non-equivalent control group pretest–posttest study with a quasi-experimental design is adopted for the assignment of experimental participants and the design of the course. The results show that a curriculum design with computational thinking significantly improves the effectiveness of students’ learning in digital technology and is especially helpful for the cultivation of the key capabilities of menu design and cost planning among restaurant management skills. The study makes the following contributions: during the Culinary and Restaurant Management course, the use of the E-learning platform and computing programs such as Microsoft Excel is associated with greater learning effectiveness than traditional teaching methods. The research results can serve as a reference for promoting an E-catering business model and a sustainable educational model in the future.
Automatic Actuation of the Anti-Freezing System Using SMA Coil Springs
Authors: Cho Daehwan , Park Joonhong , Kim Jaeil
Published: September 2021

Studies have been actively conducted on systems that prevent the breakage of water pipes from freezing in winter. Shape memory alloy (SMA) coil springs have been used as the key components of actuators that can operate automatically by detecting the real-time outside temperature changes, but research on its use as an actuator that can operate at sub-zero temperatures is insufficient. This study proposes the anti-freezing system using Ni-44.08Ti-1.46Co (wt.%) SMA coil springs that operate near sub-zero temperatures to prevent the freezing accident of water pipes. After fabricating the SMA coil springs, the test for performance evaluation of the springs applied static load conditions was conducted on the specific outside temperature. To examine the operation of anti-freezing systems applied the SMA coil spring as an actuator, the water discharge test (WDT) was also conducted along with the computational fluid simulation. The results of water discharge measurement obtained by WDT, simulations, and theoretical equations applied to the fluid resupply system constructed were compared with each other to verify the reliability. Consequently, it was confirmed that water discharge can be automatically controlled in real time according to temperature changes of SMA coil springs in the anti-freezing system.
Microstructure and Mechanical Properties of Spark Plasma Sintered and Severely Deformed AA7075 Alloy
Authors: Garbiec Dariusz , Leshchynsky Volf , García-Junceda Andrea , Swadźba Radosław , Siwak Piotr , Adamek Grzegorz , Radwański Krzysztof
Published: September 2021

In this paper, the microstructure and mechanical properties of AA7075 with a coarse-fine-grained laminated microstructure produced by spark plasma sintering (SPS) and the cyclic extrusion severe deformation (KOBO) technique were investigated. It was found that an inhomogeneous grain microstructure was formed from coarse and fine grains by the SPS process and then was transformed into a coarse-fine-grained laminated microstructure by means of KOBO extrusion at room temperature. The grain refinement during KOBO extrusion resulted in a fine grained laminated microstructure created due to the formation of low-angle grain boundaries (LAGBs), followed by dynamic recrystallization, leading to high-angle grain boundaries (HAGBs). The EBSD analysis results reveal the formation of a deformed and partially recrystallized ultrafine grain microstructure owing to the generation and development of shear bands during KOBO extrusion. The ultimate tensile strength (UTS) of the AA7075 alloy rose after SPS-KOBO severe deformation up to 422 MPa, with high strains of about 33%. The obtained results clearly show that the SPS-KOBO extrusion technique allows a bimodal laminated fine gradient grain microstructure to be obtained due to deformation and dynamic recrystallization, which result in both high strength and good ductility. The new heterogeneous AA7075 alloys obtained by the SPS-KOBO combined techniques demonstrate that microstructural heterogeneities can assist in overcoming the strength–ductility trade-off.