bluuebunny/crossref_metadata_2025 huggingface dataset:
Socioeconomic Status and Non-Fatal Adult Injuries in Selected Atlanta (Georgia USA) Hospitals
Authors: Hulland Erin , Chowdhury Ritam , Sarnat Stefanie , Chang Howard H. , Steenland Kyle
Published: March 2017

Background

Injury mortality data for adults in the United States and other countries consistently show higher mortality for those with lower socioeconomic status (SES). Data are sparse regarding the role of SES among adult, non-fatal US injuries. The current study estimated non-fatal injury risk by household income using hospital emergency department (ED) visits.

Methods

A total of 1,308,892 ED visits at 10 Atlanta (Georgia USA) hospitals from 2001-2004 (347,866 injuries) were studied. The SES was based on US census-block group income, with subjects assigned to census blocks based on reported residence. Logistic regression was used to determine risk by SES for injuries versus all other ED visits, adjusting for demographics, hospital, and weather. Supplemental analyses using hospital data from 2010-2013, without data on SES, were conducted to determine whether earlier patterns by race, age, and gender persisted.

Results

Risk for many injury categories increased with higher income. Odds ratio by quartiles of increasing income (lowest quartile as referent, 95% confidence interval [CI] given for upper most quartile) were 1.00, 1.23, 1.34, 1.40 (95% CI 1.36-1.45) for motor vehicle accidents; 1.00, 1.03, 1.11, 1.24 (95% CI 1.20-1.29) for being struck by objects; 1.00. 0.99, 1.04, 1.12 (95% CI 1.00-1.25) for suicide; and 1.00, 1.03, 1.05, 1.12 (95% CI 1.09-1.15) for falls. In contrast, decreased injury risk with increased household income was seen for assaults (1.00, 0.83, 0.73, 0.67 [95% CI 0.63-0.72], by increasing quartiles). These trends by income did not differ markedly by race and gender. Whites generally had less risk of injuries, with the exception of assaults and motor vehicle accidents. Males had higher risk of injury than females, with the exception of falls and suicide attempts. Patterns of risk for race, age, and gender were consistent between 2001-2004 and 2010-2013.

Conclusion

For most non-fatal injuries, those with higher income had more risk of ED visits, although the opposite was true for assault.

HullandE, ChowdhuryR, SarnatS, ChangHH, SteenlandK. Socioeconomic status and non-fatal adult injuries in selected Atlanta (Georgia USA) hospitals. Prehosp Disaster Med. 2017;32(4):403–413.
Do Political Science Majors Succeed in the Labor Market?
Authors: Lewis Gregory B.
Published: March 2017

Despite some stakeholders’ concerns about the practical value of an undergraduate degree in political science, our graduates actually do quite well in the labor market. Based on analysis of a sample of 3.4 million college graduates (including 86,000 in political science) from the 2009–2014 American Community Surveys, our majors earn two-thirds more than demographically similar high school graduates if they stop with bachelor’s degrees, but they are among the most likely to obtain graduate degrees, especially in law. Only engineering, economics, computer science, and health science majors make at least 10% more than our graduates, who make nearly as much as those who major in business and 10% to 25% more than those who major in most other social sciences and humanities. Political science majors have relatively high unemployment rates in their 20s, however, and may end up in very different occupations than they imagined when they chose political science.
Incentives for Sharing Knowledge: A Survey of Scholarly Practices in Public Affairs and Administration
Authors: Neely Stephen R. , Coggburn Jerrell D.
Published: March 2017

This study examines the scholarly practice of “knowledge sharing” and the extent to which it is rewarded by prevailing faculty-incentive structures. Following recent calls for greater connectivity between the academic and practitioner communities in both political science and public administration, there is a need for greater empirical evidence regarding the extent to which these practices are being employed across the discipline and how their use varies across institutional settings. Focusing on “knowledge sharing” as a specific dimension of “engaged scholarship,” this article reports the findings from a recent survey of public affairs and administration program leaders regarding current standards for earning tenure and promotion. Relevant scholarly practices are discussed and the reported value of these practices for earning tenure and promotion is presented. Institutional types are compared and recommendations are made for improving the dissemination of scholarly knowledge to the public administration practitioner community.
Professor Favorability and Student Perceptions of Professor Ideology
Authors: Braidwood Travis , Ausderan Jacob
Published: March 2017

Academia has become an increasingly common political target, particularly the institution of academic tenure, which many conservative politicians accuse of helping to perpetuate the ideological indoctrination of students. This study focuses on students’ perceptions of professors’ ideology by examining the link between student ideology, professor favorability, and perceptions of professors’ ideology. We employ an original survey instrument and find that, rather than forming perceptions of their professors’ political views based on their professors’ actual positions, students tend to project their own ideology onto their professor, based on the extent to which they like their professor.
Negating the Gender Citation Advantage in Political Science
Authors: Atchison Amy L.
Published: March 2017

Open-access (OA) advocates have long promoted OA as an egalitarian alternative to traditional subscription-based academic publishing. The argument is simple: OA gives everyone access to high-quality research at no cost. In turn, this should benefit individual researchers by increasing the number of people reading and citing academic articles. As the OA movement gains traction in the academy, scholars are investing considerable research energy to determine whether there is an OA citation advantage—that is, does OA increase an article’s citation counts? Research indicates that it does. Scholars also explored patterns of gender bias in academic publishing and found that women are cited at lower rates in many disciplines. Indeed, in many disciplines, men enjoy a significant and positive gender citation effect (GCE) compared to their female colleagues. This article combines these research areas to determine whether the OA citation advantage varies by gender. Using Wilcoxon-Mann-Whitney (WMW) tests, the nonparametric analog to the independent samples T-test, I conclude that OA benefits male and female political scientists at similar rates. Thus, OA negates the gender citation advantage that typically accrues to male political scientists.
Do you know the extinction in your young massive cluster?
Authors: De Marchi Guido , Panagia Nino , Sabbi Elena
Published: March 2017

Up to ages of ~ 100 Myr, massive clusters are still swamped in large amounts of gas and dust, with considerable and uneven levels of extinction. At the same time, large grains (ices?) produced by type II supernovae profoundly alter the interstellar medium (ISM), thus resulting in extinction properties very different from those of the diffuse ISM. To obtain physically meaningful parameters of stars, from basic luminosities and effective temperatures to masses and ages, we must understand and measure the local extinction law. This problem affects all the massive young clusters discussed in his volume.
Massive star clusters in high-redshift star-forming galaxies seen at a 100 pc scale thanks to strong gravitational lensing
Authors: Dessauges-Zavadsky Miroslava , Cava Antonio , Tamburello Valentina , Schaerer Daniel , Mayer Lucio , Richard Johan , González Pablo G. Pérez
Published: March 2017

High-resolution imaging reveals clumpy morphologies among z = 1 – 3 galaxies. Most of these galaxies are dominated by disk rotation, which led to conclude that the observed clumps are generated from disk fragmentation due to gravitational instability. Despite the kpc-scale resolution attained by the most advanced facilities and numerical simulations, these clumps are barely resolved at z > 1. Thanks to the stretching and magnification power provided by gravitational lensing, we reach the sub-kpc resolving power to unveil their physics. From our literature compilation of data, we show that without lensing there is a bias toward clumps with high masses and sizes. The high-redshift clumps identified in lensed galaxies have stellar masses 2 orders of magnitude lower and a median size of 250 pc. They resemble local star clusters observed in the most intensively star-forming galaxies. The clump masses and sizes observed in lensed galaxies agree with new simulations, which show that the Toomre instability criterion overestimates the clump masses by a factor of 5 – 6.
Stellar population properties of the most massive globular clusters and ultra-compact dwarf galaxies of the Fornax cluster
Authors: Hilker Michael
Published: March 2017

Most ultra-compact dwarf galaxies (UCDs) and very massive globular clusters reside in nearby galaxy clusters or around nearby giant galaxies. Due to their distance (> 4 Mpc) and compactness (reff< 100 pc) they are barely resolved, and thus it is difficult to obtain their internal properties. Here I present our most recent attempts to constrain the mass function, stellar content and dynamical state of UCDs in the Fornax cluster. Thanks to radial velocity membership assignment of ~ 950 globular clusters (GCs) and UCDs in the core of Fornax, the shape of their mass function is well constrained. It is consistent with the ‘standard’ Gaussian mass function of GCs. Our recent simulations on the disruption process of nucleated dwarf galaxies in cluster environments showed that ~ 40% of the most massive UCDs should originate from nuclear star clusters. Some Fornax UCDs actually show evidence for this scenario, as revealed by extended low surface brightness disks around them and onsets of tidal tails. Multi-band UV to optical imaging as well as low to medium resolution spectroscopy revealed that there exist UCDs with youngish ages, (sub-)solar [α/Fe] abundances, and probably He-enriched populations.
The SUNBIRD survey: characterizing the super star cluster populations of intensely star-forming galaxies
Authors: Randriamanakoto Zara , Väisänen Petri
Published: March 2017

Super star clusters (SSCs) represent the youngest and most massive form of known gravitationally bound star clusters in the Universe. They are born abundantly in environments that trigger strong and violent star formation. We investigate the properties of these massive SSCs in a sample of 42 nearby starbursts and luminous infrared galaxies. The targets form the sample of the SUperNovae and starBursts in the InfraReD (SUNBIRD) survey that were imaged using near-infrared (NIR) K-band adaptive optics mounted on the Gemini/NIRI and the VLT/NaCo instruments. Results from i) the fitted power-laws to the SSC K-band luminosity functions, ii) the NIR brightest star cluster magnitude − star formation rate (SFR) relation and iii) the star cluster age and mass distributions have shown the importance of studying SSC host galaxies with high SFR levels to determine the role of the galactic environments in the star cluster formation, evolution and disruption mechanisms.
Globular clusters and the evolution of their multiple stellar populations
Authors: Chantereau W. , Charbonnel C. , Meynet G.
Published: March 2017

Our knowledge of the formation and early evolution of globular clusters (GCs) has been totally shaken with the discovery of the peculiar chemical properties of their long-lived host stars. Therefore, the interpretation of the observed Colour Magnitude Diagrams (CMD) and of the properties of the GC stellar populations requires the use of new stellar models computed with relevant chemical compositions. In this paper we use the grid of evolution models for low-mass stars computed by Chantereau et al. (2015) with the initial compositions of second-generation stars as predicted by the fast rotating massive stars scenario to build synthesis models of GCs. We discuss the implications of the assumed initial chemical distribution on 13 Gyr isochrones. We build population synthesis models to predict the fraction of stars born with various helium abundances in present day globular clusters (assuming an age of 13 Gyr). With the current assumptions, 61 % of stars on the main sequence are predicted to be born with a helium abundance in mass fraction, Yini, smaller than 0.3 and only 11 % have a Yini larger than 0.4. Along the horizontal branch, the fraction of stars with Yini inferior to 0.3 is similar to that obtained along the main sequence band (63 %), while the fraction of very He-enriched stars is significantly decreased (only 3 % with Yini larger than 0.38).
The Imprints Of Galactic Environment On Cluster Formation and Evolution
Authors: Adamo Angela
Published: March 2017

Young star clusters (YSCs) appear to be a ubiquitous product of star formation in local galaxies, thus, they can be used to study the star formation process at work in their host galaxies. Moreover, YSCs are intrinsically brighter that single stars, potentially becoming the most important tracers of the recent star formation history in galaxies in the local Universe. In local galaxies, we also witness the presence of a large population of evolved star clusters, commonly called globular clusters (GCs). GCs peak formation history is very close to the redshift (z ~ 2) when the cosmic star formation history reached the maximum. Therefore, GCs are usually associated to extreme star formation episodes in high-redshift galaxies. It is yet not clear whether YSCs and GCs share a similar formation process (same physics under different interstellar medium conditions) and evolution process, and whether the former can be used as progenitor analogs of the latter. In this invited contribution, I review general properties of YSC populations in local galaxies. I will summarise some of the current open questions in the field, with particular emphasis to whether or not galactic environments, where YSCs form, leave imprints on the nested populations. The importance of this rapidly developing field can be crucial in understanding GC formation and possibly the galactic environment condition where this ancient population formed.
Environmental effects on stellar populations of star clusters and dwarf galaxies
Authors: Pasetto Stefano , Cropper Mark , Fujita Yutaka , Chiosi Cesare , Grebel Eva K.
Published: March 2017

We investigate the competitive role of the different dissipative phenomena acting on the onset of star formation of gravitationally bound systems in an external environment. Ram pressure, Kelvin-Helmholtz and Rayleigh-Taylor instabilities, and tidal forces are accounted for separately in an analytical framework and compared in their role in influencing the star forming regions. We present an analytical criterion to elucidate the dependence of star formation in a spherical stellar system on its surrounding environment. We consider the different signatures of these phenomena in synthetically realized colour-magnitude diagrams (CMDs) of the orbiting system thus investigating the detectability limits of these different effects for future observational projects and their relevance. The developed theoretical framework has direct applications to the cases of massive star clusters, dwarf galaxies in galaxy clusters and dwarf galaxies orbiting our Milky Way system, as well as any primordial gas-rich cluster of stars orbiting within its host galaxy.
Massive cold cloud clusters
Authors: Toth L. Viktor , Zahorecz Sarolta , Marton Gabor , Ristorcelli Isabelle , Juvela Mika , Montier Ludovic , Bernard Jean-Philippe
Published: March 2017

The all-sky Planck catalogue of Galactic Cold Clumps (PGCC) allows an almost unbiased study of the early phases of star-formation in our Galaxy. Several thousand of the clumps have also distance estimates allowing a determination of mass and density. The nature of Planck cold clumps varies from IRDCs to tiny nearby cold clouds with masses ranging from one to several tens of thousands solar masses. Some of the clumps are embedded in GMCs, others are isolated. Some are close or even very close to OB associations, while others lay far from any UV luminous objects. The small scale clustering of nearby PGCCs was studied with the improved Minimum Spanning Tree (MST) method identifying groups in 3D space, locating also massive cold cloud clusters eg. PGCCMST G210.6-19.5 in LDN 1641.
Are Galactic globular cluster AGB stars rich or poor in Sodium? Sodium abundance of AGB and RGB stars in NGC 2808
Authors: Wang Yue , Primas Francesca , Charbonnel Corinne , Van der Swaelmen Mathieu , Chantereau William , Bono Giuseppe
Published: March 2017

A spectroscopic study comparing the [Na/Fe] distributions of RGB and AGB stars in the Galactic globular cluster (GC) NGC 6752 found that there was no Na-rich, 2nd-generation star along the early-AGB of this cluster. This came as a surprise since in this GC, as well as other Galactic GCs studied so far, 1st- and 2nd-generation stars have usually been found from the main sequence turnoff up to the red giant branch. To investigate whether the failure of a significant fraction of stars to ascend the AGB also happens to other GCs, we studied a sample of AGB and RGB stars in NGC 2808 observed at the ESO/VLT with FLAMES. Contrary to NGC 6752, we find that the AGB and RGB stars we studied in NGC 2808 have comparable [Na/Fe] dispersions.
Dissecting 30 Doradus: Optical and Near Infrared Star Formation History of the starburst cluster NGC2070 from the Hubble Tarantula Treasury Project
Authors: Cignoni Michele ,   the HTTP team
Published: March 2017

I will present new results on the star formation history of 30 Doradus in the Large Magellanic Cloud based on the panchromatic imaging survey Hubble Tarantula Treasury Project (HTTP). Here the focus is on the starburst cluster NGC2070. The star formation history is derived by comparing the deepest ever optical and NIR color-magnitude diagrams (CMDs) with state-of-the-art synthetic CMDs generated with the latest PARSEC models, which include all stellar phases from pre-main sequence to post-main sequence. For the first time in this region we are able to measure the star formation using intermediate and low mass stars simultaneously. Our results suggest that NGC2070 experienced a prolonged activity. I will discuss the detailed star formation history, initial mass function and reddening distribution.
Mid-infrared [NeII] Imaging of Young Massive Star Clusters Near Galactic Nuclei
Authors: Yeh Sherry C. C. , Tsai Chao-Wei , Geballe Thomas R. , Herrera Cinthya N.
Published: March 2017

We investigate the formation of young massive clusters near the nuclei in NGC 6946, IC 342, Maffei II, and NGC 7714, using ground-based mid-infrared [NeII] imaging. We derive the cluster formation efficiency and cluster mass function, and the results suggest that environmental effects on YMC formation may not be significant.
Overexpression of EZH2 in multiple myeloma is associated with poor prognosis and dysregulation of cell cycle control
Authors: Pawlyn C , Bright M D , Buros A F , Stein C K , Walters Z , Aronson L I , Mirabella F , Jones J R , Kaiser M F , Walker B A , Jackson G H , Clarke P A , Bergsagel P L , Workman P , Chesi M , Morgan G J , Davies F E
Published: March 2017

Myeloma is heterogeneous at the molecular level with subgroups of patients characterised by features of epigenetic dysregulation. Outcomes for myeloma patients have improved over the past few decades except for molecularly defined high-risk patients who continue to do badly. Novel therapeutic approaches are, therefore, required. A growing number of epigenetic inhibitors are now available including EZH2 inhibitors that are in early-stage clinical trials for treatment of haematological and other cancers with EZH2 mutations or in which overexpression has been correlated with poor outcomes. For the first time, we have identified and validated a robust and independent deleterious effect of high EZH2 expression on outcomes in myeloma patients. Using two chemically distinct small-molecule inhibitors, we demonstrate a reduction in myeloma cell proliferation with EZH2 inhibition, which leads to cell cycle arrest followed by apoptosis. This is mediated via upregulation of cyclin-dependent kinase inhibitors associated with removal of the inhibitory H3K27me3 mark at their gene loci. Our results suggest that EZH2 inhibition may be a potential therapeutic strategy for the treatment of myeloma and should be investigated in clinical studies.
Hidden diversity revealed by genome-resolved metagenomics of iron-oxidizing microbial mats from Lō’ihi Seamount, Hawai’i
Authors: Fullerton Heather , Hager Kevin W , McAllister Sean M , Moyer Craig L
Published: March 2017

The Zetaproteobacteria are ubiquitous in marine environments, yet this class of Proteobacteria is only represented by a few closely-related cultured isolates. In high-iron environments, such as diffuse hydrothermal vents, the Zetaproteobacteria are important members of the community driving its structure. Biogeography of Zetaproteobacteria has shown two ubiquitous operational taxonomic units (OTUs), yet much is unknown about their genomic diversity. Genome-resolved metagenomics allows for the specific binning of microbial genomes based on genomic signatures present in composite metagenome assemblies. This resulted in the recovery of 93 genome bins, of which 34 were classified as Zetaproteobacteria. Form II ribulose 1,5-bisphosphate carboxylase genes were recovered from nearly all the Zetaproteobacteria genome bins. In addition, the Zetaproteobacteria genome bins contain genes for uptake and utilization of bioavailable nitrogen, detoxification of arsenic, and a terminal electron acceptor adapted for low oxygen concentration. Our results also support the hypothesis of a Cyc2-like protein as the site for iron oxidation, now detected across a majority of the Zetaproteobacteria genome bins. Whole genome comparisons showed a high genomic diversity across the Zetaproteobacteria OTUs and genome bins that were previously unidentified by SSU rRNA gene analysis. A single lineage of cosmopolitan Zetaproteobacteria (zOTU 2) was found to be monophyletic, based on cluster analysis of average nucleotide identity and average amino acid identity comparisons. From these data, we can begin to pinpoint genomic adaptations of the more ecologically ubiquitous Zetaproteobacteria, and further understand their environmental constraints and metabolic potential.
Dispersion/dilution enhances phytoplankton blooms in low-nutrient waters
Authors: Lehahn Yoav , Koren Ilan , Sharoni Shlomit , d’Ovidio Francesco , Vardi Assaf , Boss Emmanuel
Published: March 2017

Spatial characteristics of phytoplankton blooms often reflect the horizontal transport properties of the oceanic turbulent flow in which they are embedded. Classically, bloom response to horizontal stirring is regarded in terms of generation of patchiness following large-scale bloom initiation. Here, using satellite observations from the North Pacific Subtropical Gyre and a simple ecosystem model, we show that the opposite scenario of turbulence dispersing and diluting fine-scale (∼1–100 km) nutrient-enriched water patches has the critical effect of regulating the dynamics of nutrients–phytoplankton–zooplankton ecosystems and enhancing accumulation of photosynthetic biomass in low-nutrient oceanic environments. A key factor in determining ecological and biogeochemical consequences of turbulent stirring is the horizontal dilution rate, which depends on the effective eddy diffusivity and surface area of the enriched patches. Implementation of the notion of horizontal dilution rate explains quantitatively plankton response to turbulence and improves our ability to represent ecological and biogeochemical processes in oligotrophic oceans.
Cytomegalovirus infection exacerbates autoimmune mediated neuroinflammation
Authors: Vanheusden Marjan , Broux Bieke , Welten Suzanne P. M. , Peeters Liesbet M. , Panagioti Eleni , Van Wijmeersch Bart , Somers Veerle , Stinissen Piet , Arens Ramon , Hellings Niels
Published: March 2017

Cytomegalovirus (CMV) is a latent virus which causes chronic activation of the immune system. Here, we demonstrate that cytotoxic and pro-inflammatory CD4+CD28null T cells are only present in CMV seropositive donors and that CMV-specific Immunoglobulin (Ig) G titers correlate with the percentage of these cells. In vitro stimulation of peripheral blood mononuclear cells with CMVpp65 peptide resulted in the expansion of pre-existing CD4+CD28null T cells. In vivo, we observed de novo formation, as well as expansion of CD4+CD28null T cells in two different chronic inflammation models, namely the murine CMV (MCMV) model and the experimental autoimmune encephalomyelitis (EAE) model for multiple sclerosis (MS). In EAE, the percentage of peripheral CD4+CD28null T cells correlated with disease severity. Pre-exposure to MCMV further aggravated EAE symptoms, which was paralleled by peripheral expansion of CD4+CD28null T cells, increased splenocyte MOG reactivity and higher levels of spinal cord demyelination. Cytotoxic CD4+ T cells were identified in demyelinated spinal cord regions, suggesting that peripherally expanded CD4+CD28null T cells migrate towards the central nervous system to inflict damage. Taken together, we demonstrate that CMV drives the expansion of CD4+CD28null T cells, thereby boosting the activation of disease-specific CD4+ T cells and aggravating autoimmune mediated inflammation and demyelination.
Differential activation of endocrine-immune networks by arthritis challenge: Insights from colony-specific responses
Authors: Bodnar Tamara S. , Taves Matthew D. , Lavigne Katie M. , Woodward Todd S. , Soma Kiran K. , Weinberg Joanne
Published: March 2017

Rheumatoid arthritis (RA) is a chronic inflammatory condition with variable clinical presentation and disease progression. Importantly, animal models of RA are widely used to examine disease pathophysiology/treatments. Here, we exploited known vendor colony-based differences in endocrine/immune responses to gain insight into inflammatory modulators in arthritis, utilizing the adjuvant-induced arthritis (AA) model. Our previous study found that Sprague-Dawley (SD) rats from Harlan develop more severe AA, have lower corticosteroid binding globulin, and have different patterns of cytokine activation in the hind paw, compared to SD rats from Charles River. Here, we extend these findings, demonstrating that Harlan rats show reduced hypothalamic cytokine responses to AA, compared to Charles River rats, and identify colony-based differences in cytokine profiles in hippocampus and spleen. To go beyond individual measures, probing for networks of variables underlying differential responses, we combined datasets from this and the previous study and performed constrained principal component analysis (CPCA). CPCA revealed that with AA, Charles River rats show activation of chemokine and central cytokine networks, whereas Harlan rats activate peripheral immune/hypothalamic-pituitary-adrenal networks. These data suggest differential underlying disease mechanism(s), highlighting the power of evaluating multiple disease biomarkers, with potential implications for understanding differential disease profiles in individuals with RA.
Carbon and Nitrogen Speciation in N-poor C-O-H-N Fluids at 6.3 GPa and 1100–1400 °C
Authors: Sokol Alexander G. , Tomilenko Anatoly A. , Bul’bak Taras A. , Palyanova Galina A. , Sokol Ivan A. , Palyanov Yury N.
Published: March 2017

Deep carbon and nitrogen cycles played a critical role in the evolution of the Earth. Here we report on successful studying of speciation in C-O-H-N systems with low nitrogen contents at 6.3 GPa and 1100 to 1400 °C. At fO2 near Fe–FeO (IW) equilibrium, the synthesised fluids contain more than thirty species. Among them, CH4, C2H6, C3H8 and C4H10 are main carbon species. All carbon species, except for C1-C4 alkanes and alcohols, occur in negligible amounts in the fluids generated in systems with low H2O, but С15-С18 alkanes are slightly higher and oxygenated hydrocarbons are more diverse at higher temperatures and H2O concentrations. At a higher oxygen fugacity of +2.5 Δlog fO2 (IW), the fluids almost lack methane and contain about 1 rel.% C2-C4 alkanes, as well as fractions of percent of C15–18 alkanes and notable contents of alcohols and carboxylic acids. Methanimine (CH3N) is inferred to be the main nitrogen species in N-poor reduced fluids. Therefore, the behaviour of CH3N may control the nitrogen cycle in N-poor peridotitic mantle. Oxidation of fluids strongly reduces the concentration of CH4 and bulk carbon. However, higher alkanes, alcohols, and carboxylic acids can resist oxidation and should remain stable in mantle hydrous magmas.
Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
Authors: Ströhl Florian , Lin Julie Qiaojin , Laine Romain F. , Wong Hovy Ho-Wai , Urbančič Vasja , Cagnetta Roberta , Holt Christine E. , Kaminski Clemens F.
Published: March 2017

Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.
Eastern Mediterranean Mobility in the Bronze and Early Iron Ages: Inferences from Ancient DNA of Pigs and Cattle
Authors: Meiri Meirav , Stockhammer Philipp W. , Marom Nimrod , Bar-Oz Guy , Sapir-Hen Lidar , Morgenstern Peggy , Macheridis Stella , Rosen Baruch , Huchon Dorothée , Maran Joseph , Finkelstein Israel
Published: March 2017

The Late Bronze of the Eastern Mediterranean (1550–1150 BCE) was a period of strong commercial relations and great prosperity, which ended in collapse and migration of groups to the Levant. Here we aim at studying the translocation of cattle and pigs during this period. We sequenced the first ancient mitochondrial and Y chromosome DNA of cattle from Greece and Israel and compared the results with morphometric analysis of the metacarpal in cattle. We also increased previous ancient pig DNA datasets from Israel and extracted the first mitochondrial DNA for samples from Greece. We found that pigs underwent a complex translocation history, with links between Anatolia with southeastern Europe in the Bronze Age, and movement from southeastern Europe to the Levant in the Iron I (ca. 1150–950 BCE). Our genetic data did not indicate movement of cattle between the Aegean region and the southern Levant. We detected the earliest evidence for crossbreeding between taurine and zebu cattle in the Iron IIA (ca. 900 BCE). In light of archaeological and historical evidence on Egyptian imperial domination in the region in the Late Bronze Age, we suggest that Egypt attempted to expand dry farming in the region in a period of severe droughts.
THz Electric Field-Induced Second Harmonic Generation in Inorganic Ferroelectric
Authors: Grishunin Kirill A. , Ilyin Nikita A. , Sherstyuk Natalia E. , Mishina Elena D. , Kimel Alexey , Mukhortov Vladimir M. , Ovchinnikov Andrey V. , Chefonov Oleg V. , Agranat Mikhail B.
Published: March 2017

Second Harmonic Generation induced by the electric field of a strong nearly single-cycle terahertz pulse with the peak amplitude of 300 kV/cm is studied in a classical inorganic ferroelectric thin film of (Ba0.8Sr0.2)TiO3. The dependences of the SHG intensity on the polarization of the incoming light is revealed and interpreted in terms of electric polarization induced in the plane of the film. As the THz pulse pumps the medium in the range of phononic excitations, the induced polarization is explained as a dynamical change of the ferrolectric order parameter. It is estimated that under action of the THz pulse the ferroelectric order parameter acquires an in-plane component up to 6% of the net polarization.
Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton
Authors: Zubair Muhammad , Zaidi Syed Shan-e-Ali , Shakir Sara , Farooq Muhammad , Amin Imran , Scheffler Jodi A. , Scheffler Brian E. , Mansoor Shahid
Published: March 2017

The first epidemic of cotton leaf curl disease (CLCuD) in early 1990’s in the Indian subcontinent was associated with several distinct begomoviruses along with a disease-specific betasatellite. Resistant cotton varieties were introduced in late 1990’s but soon resistance was broken and was associated with a single recombinant begomovirus named Burewala strain of Cotton leaf curl Kokhran virus that lacks a full complement of a gene encoding a transcription activator protein (TrAP). In order to understand the ongoing changes in CLCuD complex in Pakistan, CLCuD affected plants from cotton fields at Vehari were collected. Illumina sequencing was used to assess the diversity of CLCuD complex. At least three distinct begomoviruses characterized from the first epidemic; Cotton leaf curl Multan virus, Cotton leaf curl Kokhran virus and Cotton leaf curl Alabad virus, several distinct species of alphasatellites and cotton leaf curl Multan betasatellite were found associated with CLCuD. These viruses were also cloned and sequenced through Sanger sequencing to confirm the identity of the begomoviruses and that all clones possessed a full complement of the TrAP gene. A new strain of betasatellite was identified here and named CLCuMuBVeh. The implications of these findings in efforts to control CLCuD are discussed.
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
Authors: Toro Nicolás , Villadas Pablo J. , Molina-Sánchez María Dolores , Navarro-Gómez Pilar , Vinardell José M. , Cuesta-Berrio Lidia , Rodríguez-Carvajal Miguel A.
Published: March 2017

The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.
Cancer-associated noncoding mutations affect RNA G-quadruplex-mediated regulation of gene expression
Authors: Zeraati Mahdi , Moye Aaron L. , Wong Jason W. H. , Perera Dilmi , Cowley Mark J. , Christ Daniel U. , Bryan Tracy M. , Dinger Marcel E.
Published: March 2017

Cancer is a multifactorial disease driven by a combination of genetic and environmental factors. Many cancer driver mutations have been characterised in protein-coding regions of the genome. However, mutations in noncoding regions associated with cancer have been less investigated. G-quadruplex (G4) nucleic acids are four-stranded secondary structures formed in guanine-rich sequences and prevalent in the regulatory regions. In this study, we used published whole cancer genome sequence data to find mutations in cancer patients that overlap potential RNA G4-forming sequences in 5′ UTRs. Using RNAfold, we assessed the effect of these mutations on the thermodynamic stability of predicted RNA G4s in the context of full-length 5′ UTRs. Of the 217 identified mutations, we found that 33 are predicted to destabilise and 21 predicted to stabilise potential RNA G4s. We experimentally validated the effect of destabilising mutations in the 5′ UTRs of BCL2 and CXCL14 and one stabilising mutation in the 5′ UTR of TAOK2. These mutations resulted in an increase or a decrease in translation of these mRNAs, respectively. These findings suggest that mutations that modulate the G4 stability in the noncoding regions could act as cancer driver mutations, which present an opportunity for early cancer diagnosis using individual sequencing information.
The fractal organization of ultradian rhythms in avian behavior
Authors: Guzmán Diego A. , Flesia Ana G. , Aon Miguel A. , Pellegrini Stefania , Marin Raúl H. , Kembro Jackelyn M.
Published: March 2017

Living systems exhibit non-randomly organized biochemical, physiological, and behavioral processes that follow distinctive patterns. In particular, animal behavior displays both fractal dynamics and periodic rhythms yet the relationship between these two dynamic regimens remain unexplored. Herein we studied locomotor time series of visually isolated Japanese quails sampled every 0.5 s during 6.5 days (>106data points). These high-resolution, week-long, time series enabled simultaneous evaluation of ultradian rhythms as well as fractal organization according to six different analytical methods that included Power Spectrum, Enright, Empirical Mode Decomposition, Wavelet, and Detrended Fluctuation analyses. Time series analyses showed that all birds exhibit circadian rhythms. Although interindividual differences were detected, animals presented ultradian behavioral rhythms of 12, 8, 6, 4.8, 4 h and/or lower and, irrespective of visual isolation, synchronization between these ultradian rhythms was observed. Moreover, all birds presented similar overall fractal dynamics (for scales ∼30 s to >4.4 h). This is the first demonstration that avian behavior presents fractal organization that predominates at shorter time scales and coexists with synchronized ultradian rhythms. This chronobiological pattern is advantageous for keeping the organism’s endogenous rhythms in phase with internal and environmental periodicities, notably the feeding, light-dark and sleep-wake cycles.
Modelling the three-dimensional structure of the right-terminal domain of pospiviroids
Authors: Steger Gerhard
Published: March 2017

Viroids, the smallest know plant pathogens, consist solely of a circular, single-stranded, non-coding RNA. Thus for all of their biological functions, like replication, processing, and transport, they have to present sequence or structural features to exploit host proteins. Viroid binding protein 1 (Virp1) is indispensable for replication of pospiviroids, the largest genus of viroids, in a host plant as well as in protoplasts. Virp1 is known to bind at two sites in the terminal right (TR) domain of pospiviroids; each site consists of a purine- (R-) and a pyrimidine- (Y-)rich motif that are partially base-paired to each other. Here we model the important structural features of the domain and show that it contains an internal loop of two Y · Y cis Watson-Crick/Watson-Crick (cWW) pairs, an asymmetric internal loop including a cWW and a trans Watson/Hoogsteen pair, and a thermodynamically quite stable hairpin loop with several stacking interactions. These features are discussed in connection to the known biological functions of the TR domain.
Preovulatory suppression of mouse oocyte cell volume-regulatory mechanisms is via signalling that is distinct from meiotic arrest
Authors: Richard Samantha , Baltz Jay M.
Published: March 2017

GLYT1-mediated glycine transport is the main cell volume-homeostatic mechanism in mouse eggs and early preimplantation embryos. It is unique to these developmental stages and key to their healthy development. GLYT1 first becomes activated in oocytes only after ovulation is triggered, when meiotic arrest of the oocyte is released, but how this occurs was unknown. Here we show that GLYT1 activity is suppressed in oocytes in the preovulatory antral follicle and that its suppression is mediated by a mechanism distinct from the gap junction-dependent Natriuretic Peptide Precursor C (NPPC) pathway that controls meiotic arrest. GLYT1 remained suppressed in isolated antral follicles but not isolated cumulus-oocyte complexes (COCs) or isolated oocytes. Moreover, activating the NPPC signalling pathway could not prevent GLYT1 activation in oocytes within COCs despite maintaining meiotic arrest. Furthermore, blocking gap junctions in isolated follicles failed to induce GLYT1 activity in enclosed oocytes for an extended period after meiosis had resumed. Finally, isolated mural granulosa cells from preovulatory antral follicles were sufficient to suppress GLYT1 in oocytes within co-cultured COCs. Together, these results suggest that suppression of GLYT1 activity before ovulation is mediated by a novel signalling pathway likely originating from preovulatory mural granulosa cells.
Salvia fruticosa Induces Vasorelaxation In Rat Isolated Thoracic Aorta: Role of the PI3K/Akt/eNOS/NO/cGMP Signaling Pathway
Authors: Anwar M. Akhtar , Samaha Ali A. , Ballan Samar , Saleh Alaaeldin I. , Iratni Rabah , Eid Ali H.
Published: March 2017

Salvia fruticosa (SF) Mill. is traditionally used for its antihypertensive actions. However, little is known about its pharmacologic and molecular mechanisms of action. Here we determined the effects of an ethanolic extract of SF leaves on rings of isolated thoracic aorta from Sprague-Dawley rats. Our results show that SF extract increased nitric oxide production and relaxed endothelium-intact rings in a dose-dependent (0.3 µg/ml–1 mg/ml) manner, and the maximum arterial relaxation (Rmax) was significantly reduced with endothelium denudation. Pretreatment of endothelium-intact rings with L-NAME (a non-selective inhibitor of nitric oxide synthase, 100 µM), or ODQ (an inhibitor of soluble guanylyl cyclase, 10 µM) significantly diminished SF-mediated vasorelaxation. Furthermore, SF induced Akt phosphorylation as well as increased cGMP levels in rings treated with increasing doses of SF. Prior exposure to PI3K inhibitors, wortmannin (0.1 µM) or LY294002 (10 µM), decreased cGMP accumulation and attenuated the SF-induced vasorelaxation by approximately 50% (Rmax). SF-evoked relaxation was not affected by indomethacin, verapamil, glibenclamide, tetraethylammonium, pyrilamine or atropine. Taken together, our results indicate that SF induces endothelium-dependent vasorelaxation through the PI3K/Akt/eNOS/NO/sGC/cGMP signaling pathway. Our data illustrate the health-orientated benefits of consuming SF which may act as an antihypertensive agent to reduce the burden of cardiovascular complications.
Overexpression of matrix metalloproteinase-9 (MMP-9) rescues insulin-mediated impairment in the 5XFAD model of Alzheimer’s disease
Authors: Kaminari Archontia , Giannakas Nikolas , Tzinia Athina , Tsilibary Effie C.
Published: March 2017

A hallmark of Alzheimer’s disease (AD) is the accumulation of oligomeric amyloid-β (Aβ) peptide, which may be primarily responsible for neuronal dysfunction. Insulin signaling provides a defense mechanism against oligomer-induced neuronal loss. We previously described the neuroprotective role of matrix metalloproteinase 9 (MMP-9) in decreasing the formation of Aβ oligomers. In the present study, we examined the role of MMP-9 on the insulin survival pathway in primary hippocampal cultures and hippocampal cell extracts from 3 month-old wild type, AD (5XFAD), MMP-9-overexpressing (TgMMP-9), and double transgenic mice (5XFAD/TgMMP-9). The data demonstrate that the insulin pathway was compromised in samples from 5XFAD mice, when compared to the wild type and TgMMP-9. This was due to enhanced phosphorylation of IRS1 at Serine 636 (pIRS1-Ser636), which renders IRS1 inactive and prevents insulin-mediated signaling. In 5XFAD/TgMMP-9 samples, the insulin survival pathway was rescued through enhanced activation by phosphorylation of IRS1 at Tyrosine 465 (pIRS1-Tyr465), downstream increased phosphorylation of Akt and GSK-3β, and decreased phosphorylation of JNK kinase. Oligomeric Aβ levels decreased and BDNF levels increased in 5XFAD/TgMMP-9 mice, compared to 5XFAD mice. Our findings indicate that overexpression of MMP-9 rescued insulin survival signalingin vitroand in early stages in the 5XFAD model of AD.
Efficacy and safety of multimodal analgesic techniques for preventing chronic postsurgery pain under different surgical categories: a meta-analysis
Authors: Zhou Jun , Fan Youling , Zhong Jiying , Wen Xianjie , Chen Hongtao
Published: March 2017

The purpose of this meta-analysis was to compare the efficacy and safety of regional anesthesia to manage chronic postsurgery pain. A systematic search of PubMed, EmBase, and the Cochrane Central Register of Controlled Trials was performed to identify randomized controlled trials that focused on chronic pain frequency, analgesic consumption, and adverse effects under different surgical categories. We collected 21 trials assessing 1,980 patients for our meta-analysis. The summary of relative risks (RRs) and standard mean differences (SMDs) were calculated to measure the treatment effect of regional anesthesia. Results indicated that regional anesthesia significantly reduced the frequency of postsurgery pain (RR, 0.69; 95% confidence interval [CI], 0.56–0.85; p < 0.001). The results showed significant differences in overall patient satisfaction between applications with and without regional anesthesia (SMD, 1.95; 95%CI, 0.83–3.06; p = 0.001); however in other results, there were no significant differences between the two groups. Subgroup analysis suggested that regional anesthesia treatment might differ according to country. In conclusion, our study indicated that regional anesthesia was effective and safe in reducing the frequency of postsurgery pain and improved overall patient satisfaction; however, studies on the long-term efficacy and safety of regional anesthesia are still required to further confirm these findings.
Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway
Authors: Wei Chun-Chun , Kong Yuan-Yuan , Li Guo-Qiang , Guan Yun-Feng , Wang Pei , Miao Chao-Yu
Published: March 2017

Replenishment of NAD+ has been shown to protect against brain disorders such as amyotrophic lateral sclerosis and ischemic stroke. However, whether this intervention has therapeutic effects in intracerebral hemorrhage (ICH) is unknown. In this study, we sought to determine the potential therapeutic value of replenishment of NAD+ in ICH. In a collagenase-induced ICH (cICH) mouse model, nicotinamide mononucleotide (NMN), a key intermediate of nicotinamide adenine dinucleotide (NAD+) biosynthesis, was administrated at 30 minutes post cICH from tail vein to replenish NAD+. NMN treatment did not decrease hematoma volume and hemoglobin content. However, NMN treatment significantly reduced brain edema, brain cell death, oxidative stress, neuroinflammation, intercellular adhesion molecule-1 expression, microglia activation and neutrophil infiltration in brain hemorrhagic area. Mechanistically, NMN enhanced the expression of two cytoprotective proteins: heme oxygenase 1 (HO-1) and nuclear factor-like 2 (Nrf2). Moreover, NMN increased the nuclear translocation of Nrf2 for its activation. Finally, a prolonged NMN treatment for 7 days markedly promoted the recovery of body weight and neurological function. These results demonstrate that NMN treats brain injury in ICH by suppressing neuroinflammation/oxidative stress. The activation of Nrf2/HO-1 signaling pathway may contribute to the neuroprotection of NMN in ICH.