bluuebunny/crossref_metadata_2025 huggingface dataset:
Intralesional interferon-alpha 2b treatment of basal cell carcinoma.
Authors: Thestrup-Pedersen K , Jacobsen I E , Frentz G
Published: July 2023

Ten patients with histologically proven basal cell carcinoma were treated with sublesional injections of interferon-alpha 2 b (Introna), 1.5 x 10(6) units per injection, three times per week for 3 weeks. Six of the treated lesions cleared completely without further therapy. These lesions measured less than 19 x 19 mm. There was no relapse during an observation period of up to 21 months. Non-healing tumours were large (greater than 20 x 20 mm) and two of them were of nodular type. Side effects were negligible, if present at all. Introna seems to offer a valuable alternative treatment for selected cases of basal cell carcinoma, when excision or X-ray therapy cannot be performed.
Beneficial response to megoestrol acetate in AIDS-related cachexia and a possible megoestrol withdrawal-associated syndrome?
Authors: Nathwani D , Green S T , Heslop J M , Goldberg D J , Kennedy D H
Published: July 2023

A man with AIDS is described in whom a profound weight loss was converted into a weight gain by treatment with megoestrol acetate, a synthetic progesterone. His appetite improved and was accompanied by a feeling of improved well-being. Following abrupt discontinuation of the drug, there was a significant but transient depression of mood and appetite associated with loss of energy; it is suggested that this complex of symptoms might represent a megoestrol acetate withdrawal-associated syndrome.
Studies on zinc in wound healing
Authors: Ågren MS
Published: July 2023

Topical zinc is widely used in wound treatment although the beneficial effect of zinc has only been documented in zinc-deficient patients who were given zinc orally. The main purpose of this study was to investigate the effect of topically applied zinc on leg ulcer healing and examine its effect on some mechanisms in wound healing using standardized animal models. Additionally, absorption of zinc into wounds and intact skin treated topically with zinc was studied. In a double-blind trial involving 37 leg ulcer patients with low serum zinc levels, topical zinc oxide promoted cleansing and re-epithelialization. Infections and deteriorations of ulcers were less common in zinc oxide treated patients. Re-epithelialization, an important mechanism in the closure of leg ulcers, was enhanced with zinc oxide applied topically on partial-thickness wounds in pigs with normal zinc status. Zinc sulfate at three different concentrations did not, however, result in this beneficial effect on the resurfacing of wounds. The inflammatory reaction was diminished in zinc treated wounds except when a high zinc sulfate concentration was applied. Bacterial growth and concomitant diseases such as diabetes can complicate wound healing. In normal rats, bacterial growth in full-thickness wounds was reduced with topical zinc oxide but not in hyperglycemic diabetic rats. The anti-bacterial mechanism of zinc oxide seemed to be more indirect and to be mediated via local defense systems rather than being directly toxic to the bacteria. Healing of 21-day-old skin incisions was impaired in zinc deficiency, as measured by a significantly decreased wound breaking strength in zinc-deficient rats compared with that of pair-fed controls. The decreased breaking strength did not seem to be due to differences in collagen concentration of the wounds. Zinc oxide was slowly but continuously solubilized when applied on open wounds in rats. On the other hand, with zinc sulfate, the zinc concentrations, either locally or systemically, did not maintain a constant level for the 48-hour post-operative treatment period as they did with zinc oxide. Zinc absorption in and through normal human forearm skin was demonstrated after treatment with a zinc oxide medicated occlusive dressing by increased zinc levels in epidermis, interstitial fluid and dermis compared with the non-zinc control dressing. In conclusion, topical zinc may stimulate leg ulcer healing by enhancing re-epithelialization, decreasing inflammation and bacterial growth. When zinc is applied on wounds it not only corrects a local zinc deficit but also acts pharmacologically.
Renewal rate of human sebaceous glands
Authors: Plewig G , Christophers E.
Published: July 2023

Abstract is not availeble.
Demonstration of basement membrane autoantibodies in patients with benign mucous membrane pemphigoid
Authors: Dabelsteen E , Ullman S , Thomsen K , Rygaard J.
Published: July 2023

Abstract is not availeble.
Elution of antibody from the skin lesions of patients with pemphigus vulgaris
Authors: Nishikawa T , Harada T , Hatano H , Ogawa H , Miyazaki H.
Published: July 2023

Abstract is not availeble.
Photohemolytic activity of tryptophan and phenylalanine metabolites
Authors: Swanbeck G , Wennersten G.
Published: July 2023

Abstract is not availeble.
Cellular immunodeficiency in anhidrotic ectodermal dysplasia
Authors: Davis JR , Solomon LM.
Published: July 2023

By using in vitro methods of patients with anhidrotic ectodermal dysplasia (AED) was shown to have depressed lymphocyte function when compared with a control group. IgE levels of the AED group were elevated above those of a control group at the p=0.01 level of significance. In vivo methods utilizing the application of DNCB demonstrated, in addition, decreased delayed hypersensitivity reactions in the anhidrotic patients. Thus there appears to be some degree of cellular immune hypofunction in patients with AED, all of whom have demonstrated at some time a lichenified dermatitis clinically indistinguishable from atopic dermatitis.
Generalized pustular psoriasis. A report on thirteen patients
Authors: Lindgren S , Groth O.
Published: July 2023

Generalized pustular psoriasis shows a fluctuating pattern, its course varying with time and from patient to patient. The prognosis and results of treatment are therefore extremely difficult to assess. The patients in this series have been followed up by us with regard to the natural course of the illness including complications and precipitating factors. Oral drug therapy has suprisingly often provoked acute pustular eruptions, and these patients have also strikingly often and rapidly developed contact allergy to topical applications. The undesirable effects of long-term systemic corticosteroid therapy have been confirmed in this series, chiefly by severe rebound phenomena, notwithstanding that such treatment has proved the only means of rapidly reversing life-threatening states. The effects of methotrexate and hydroxyurea were at times good but, not uncommonly, mediocre and occasionally even useless. Large doses of antibiotics have also failed to achieve the results described by others. On the contrary, secondary infection with beta-hemolytic streptococci has caused serious problems in several cases. Apart from careful protection from infection and precipitating factors, little can be done to influence the course of generalized pustular psoriasis, but trial with cytostatics is justified during severe bouts, pending the appearance of new lines of therapy.
Mechanism of recurrence of pigmented nevi following dermabrasion
Authors: Imagawa I , Endo M , Morishima T.
Published: July 2023

A study was made of pigment freckles which recur after skin abrasion of spotted grouped pigmented nevi, according to the lapse of time, chiefly by means of the fluorescence method of Falck and Hillarp. The mechanism of recurrence is summarized as follows. Nearly simultaneously with the epidermal regeneration, dendritic melanin-producing cells derived from hair follicles and eccrine sweat ducts appear in the basal and prickle-cell layers of epidermis. These cells then create junction activity in the basal layer of epidermis, in the hair follicles and in the eccrine sweat duct walls. Finally, these nevus cells drop off into the underlying layer of scar tissue along the epidermal appendages. The dendritic melanin-producing cells seen in the early regenerative pigment freckles were thought to be incompletely differentiated nevus cells in the pre-stage of junction nevus formation. Appearance of dendritic melanin-producing cells and formation of junction activity in the eccrine sweat duct walls suggest the following possibilities. (1) In the cases of spotted grouped pigmented nevus which we studied, nevus cell proliferation also occurred eccrine-centrically on pathogenesis. (2) An important role is played by the eccrine sweat ducts in the recurrence of ordinary pigmented nevi after incomplete removal. (3) Eccrine-centered nevus cells are derived from nevoblasts in the eccrine sweat duct walls.
Syndermatotic cataract in patients with psoriasis
Authors: Wanscher B , Vesterdal E.
Published: July 2023

266 consecutive in-patients, more than 10 years of age (mean age 24.7) and suffering from psoriasis have been examined with slit lamp and ophthalmoscope to determine the incidence of cataract among such patients. 188 (70%) had clear lenses. 66(26%) presented some minute punctate opacities considered as physiological variations. Four had blue-dot cataract, and 6 had congenital or evolutionary small opacities at various sites in the lenses but without blurring of vision. Only one patient, aged 72, had crystalline cataract with slightly impaired vision. The conclusion is drawn that the incidence of cataract among patients with psoriasis does not exceed that in the normal population. Consequently, routine eye examinations for cataract are unnecessary in these patients.
Epidermal location of beta-2-microglobulin in human skin
Authors: Forsum U , Tjernlund UM.
Published: July 2023

Beta-2-microglobulin (beta2-m) is associated with the epidermis in normal and pathological human skin and gives an immunofluorescence pattern similar to that of pemphigus antibodies and concanavalin A (Con-A). Inhibition experiments indicate, however, that the binding site of anti-beta2-m is not identical with that of pemphigus antibodies or Con-A. It is suggested that beta2-m might be an epidermal cell surface marker useful for the study of antigens associated with the epidermal cell surface.
Electron microscopic cytochemistry for demonstration of sodium fluoride sensitive adenyl cyclase in normal human epidermis
Authors: Wadskov S , Sondergaard J , Kobayasi T.
Published: July 2023

Adenyl cyclase activity in normal human epidermis was detected by an electron microscopic cytochemical technique. The sodium fluoride sensitive receptors were demonstrated on the outer sheath of the cell membranes. Adenyl cyclase activity was demonstrated in the basal cells and in the 4-5 lower layers of the Malpighian cells, while the superficial layers, stratum granulosum and stratum corneum showed no activity. The findings confirm and extend previous biochemical studies and provide a clue to the conception of the adenyl cyclase enzyme as a transmembrane arranged system.
Glutathionedopa in malignant melanoma
Authors: Agrup G , Falck B , Rorsman H , Rosengren AM , Rosengren E.
Published: July 2023

A human melanoma metastasis was found to contain a substance identical with synthetic glutathionedopa when examined by means of an automatic amino acid analyser, chromatography on a Dowex-50 column, thin-layer electrophoresis, and by fluorimetry. The presence of glutathionedopa in melanoma tissue and of enzymes capable of hydrolysing the glutathione moiety of the molecule, together with the absence of glutathionedopa in the urine, suggest that glutathionedopa is an intermediate substance in the formation of 5-S-cysteinyldopa.
Complement as an essential factor for indirect immunofluorescence in cases of pemphigoid
Authors: Bockendahl H , Remy W , Stüttgen G , Petersen G.
Published: July 2023

Hitherto in the literature no proof has been published of a complement activation on the basement membrane in cases of pemphigoid. In this paper, for the first time, it is demonstrated, even if only in vitro, that complement, whose presence in the region of the basement membrane has long been known, plays an active role in a reaction. Through the action of the haemolytic system (amboceptor and sheep erythrocytes) and of anti C3 on reaction specimens for indirect immunofluorescence in pemphigoid, it is shown that the indicator reaction of the fluorescein-labelled anti-IgG with the immune complex attached to the basement membrane is markedly dependent on complement. Free, dissolved IgG is precipitated by appropriate anti-serum, even without complement.
Deficient neutrophil function in a patient with chronic mucocutaneous candidiasis, thymoma and myasthenia gravis
Authors: Mobacken H , Lindholm L , Olling S.
Published: July 2023

The case of a 68-year-old man who suffered the onset of chronic mucocutaneous candidiasis, thymoma, myasthenia gravis and a reversible neutrophil dysfunction during adult life is reported. All tests for humoral and cellular immunity proved normal. Thymectomy and the administration of transfer factor had no effect on the skin disease. Long-term oral administration of 5-fluorocytosine almost cleared the skin lesions; the neutrophil defect disappeared.
The pigmentary response to photochemotherapy
Authors: Zaynoun S , Konrad K , Gschnait F , Wolff K.
Published: July 2023

Previous studies on skin topically photosensitized with trimethylpsoralen and subsequently irradiated with long-wave UV light have demonstrated an increase in melanosome size and changes in the distribution patterns of melanosomes, suggesting the possibility of gene derepression or the induction of a somatic mutation of melanocytes. The present investigation was performed to determine whether identical changes are induced by systemic photochemotherapy using 8-methoxypsoralen and UVA (PUVA) under therapeutic conditions. Our results show that PUVA stimulates melanogenesis but does not induce significant changes in the average size of melanosomes nor in their distribution patterns within keratinocytes. Thus they indicate that under therapeutic conditions PUVA does not induce morphologically detectable cytogenetic changes in pigment cells.
Chlamydia trachomatis infection and veneral disease
Authors: Johannisson G , Edmar B , Lycke E.
Published: July 2023

Chlamydia trachomatis was isolated by the irradiated McCoy cell technique from 44 out of 103 men with non-gonorrhoeic urethritis and from 11 out of 15 patients with post-gonococcal urethritis. In women attending the venereal diseases clinics, chlamydial infection was observed in 49 out of 130 patients (38%), an infection incidence of the same order of magnitude as the one noted for gonococcal infection (40%). In 19% both infections occurred simultaneously. Treatment with tetracycline eliminated symptoms and chlamydial infection in almost all cases. The significance of the findings is discussed.
Lichen planus: an ultrastructural study
Authors: Medenica M , Lorincz A.
Published: July 2023

Ultrastructural features of skin lesions are described in 11 patients with lichen planus. Keratinocytes and melanocytes in the basal layer show loss of nuclear membrane, disappearance of nucleoli, homogenization of nuclear material, and aggregation of tonofilaments around the nucleus to resemble cells in the late prophase of the mitotic cycle except that there is no clumping of chromosomes. These cells undergo fibrillar transformation to form densely packed fibrillar bodies (colloid bodies) which are the size and shape of normal cells and frequently have cytoplasmic organelles. Ten per cent of the mainly lympho-histiocytic cells in the dermal infiltrate show changes such as multiple cell membrane discontinuities, disintegration of cytoplasm, and breaks in the nuclear membrane with spilling of nuclear substance either into the cytoplasm or the extracellular space. The shape, size and occasional fibrillar changes in the nuclei and cytoplasm of these cells resemble colloid bodies. Contact sites frequently occurred between cells in the dermal infiltrate, especially between lymphocytes and macrophages. It appears that primary injury to the basal layer cells occurs during the early phase of the mitotic cycle and antigen from this primary site may evoke a cell-mediated type of hypersensitivity reaction. Colloid bodies evolve from cells which have injured nuclei and undergo fibrillar transformation. Dermal as well as epidermal cells may contribute to colloid body formation.
Topical treatment of penile condylomata acuminata with podophyllin, podophyllotoxin and colchicine A comparative study
Authors: Von Krogh G
Published: July 2023

The effect of alcoholic solutions with 20% podophyllin from Podophyllum peltatum and Podophyllum emodi, 8% podophyllotoxin, and 8% colchicine, when applied to penile condylomata acuminata in 227 men, were statistically alike. Of the patients initially judged to be cured after 1-2 applications, 13% showed recurrence, thus bringing down the permanent cure frequency to only 43%. Local side effects were absent after only half the series of colchicine applications, whereas as much as about 3/4 of the treatment course with podophyllin and pure podophyllotoxin could be completed without provoking discomfort. Warts in the
Serum levels of 8-methoxypsoralen 2 hours after oral administration
Authors: Steiner I , Prey T , Gschnait F , Washüttl J , Greiter F
Published: July 2023

Serum levels of 8-methoxypsoralen (8-MOP) were determined 2 hours after oral administration and found to vary considerably among psoriatic patients. There was no definite correlation between the 8-MOP serum level, the dose of 8-MOP/kg body weight ingested, and the minimal phototoxic UVA dose.
Determination of cyclic AMP in heat-separated human epidermal tissue
Authors: Wadskov S , Søndergaard J
Published: July 2023

Heat separation is introduced as a simple, reliable and quick method for obtaining pure epidermis for cyclic AMP analysis. Heating at 60 degrees -65degrees C for 10 min resulted in a distinct separation of epidermis from dermis. The level of cyclic AMP in epidermal tissue from 9 patients was 3.93 +/- 0.31 pmol per mg dry weight. The inter- and intra-assay variations were 10.3% and 8.8%, respectively. The coefficience of variation in eight biopsies from the same piece of skin was 16.2% The sensitivity of the assay was 0.07 pmol. Recovery analyses revealed that 80.5% of labelled cyclic AMP was refound after extraction and purification procedures. Addition of unlabelled cyclic AMP resulted in a recovery of approximately 100%. The specificity of the assay was high without interference from several nucleotides and the validity was confirmed by zero values obtained after addition of phosphodiesterase or charcoal.
Trichochromes in red human hair
Authors: Agrup G , Hansson C , Rorsman H , Rosengren AM , Rosengren E
Published: July 2023

The presence of trichochromes B and C in red human hair was confirmed with an analytical procedure which does not give rise to the formation of these compounds as artifacts. The trichochrome precursor 5-S-cysteinyldopa previously demonstrated in red guinea-pig hair was not detected in red human hair.
The fluroescent profiles of keratohyalin granules of newborn rat epidermis with a new fluorescent thiol reagent (DACM)
Authors: Tezuka T , Hirai R , Ogawa H
Published: July 2023

A highly specific fluorescent thiol reagent, N-(7-Dimethylamino-4-methyl-coumarinyl)maleimide (DACM) has been used to examine the newborn rat skin in order to elucidate the cystine-rich keratohyalin granules or dense homogeneous deposits. After reduction of the disulphide bond with 2-mercaptoethanol, the periphery of the stratum corneum cells showed a very distinct fluorescence and the cytoplasm of the stratum corneum, granulosum and spinulosum showed moderate fluorescence. No fluorescence was observed, however, over the keratohyalin granules themselves. Intense, spotty fluorescence was observed from the remnants of nuclei and at the periphery of large keratohyalin granules of the stratum granulosum cells, which were assumed to be dense homogeneous deposts according to their distribution pattern. As the intense fluorescence elicited with DACM determines the abundance of cystine content in the material, cystine was found to be very scarce in keratohyalin granules, but abundant in dense homogeneous deposits and at the periphery of the stratum corneum cells.
Absorption of 8-hydroxyquinolines through the human skin
Authors: Fischer T , Fagerlund C , Hartvig P
Published: July 2023

The skin absorption of clioquinol and chloroquinaldol in ointment or cream base was studied in fourteen patients with widespread dermatoses. A serum concentration in the range of 0.3--1.3 microgram/ml using clioquinol and 0.05--0.1 microgram/ml with chloroquinaldol was reached by the second day and persisted throughout the topical treatment. The mean serum half-life of clioquinol and of chloroquinaldol was estimated to 25 and 6 hours, respectively. Local application of zinc oxide ointment after the end of the treatment chelate-bound the skin deposits of clioquinol and lowered the serum half-life to 11 hours. The mean daily urinary excretion of the applied amount of clioquinol and chloroquinaldol was 4.5 and 6%, respectively.
Healing of ischaemic ulcers by intravenous prostaglandin E1 in a woman with thrombangitis obliterans
Authors: Olsson AG , Thyresson N
Published: July 2023

Abstract is not available
Dermatitis herpetiformis in Finland
Authors: Reunala T , Lokki J
Published: July 2023

During a 3 1/2-year period, 492 patients with dermatitis herpetiformis (DH) visited the dermatological departments of Finnish hospitals. The prevalence of DH in Finland was 10.4 patients/100,000 inhabitants, or 1/10,000. About 60 patients contracted DH each year, giving an incidence of 1.3 patients/100,000 inhabitants/year. These figures show that DH is a rather common disease in Finland--much more so in Finland than in Britain. Its incidence does not differ much from that approximated for coeliac disease in Finland. DH occured in all parts of the country. The birth-places of the patients were distributed evenly over the whole country, in contrast to the distribution pattern of rare hereditary diseases in Finland. No significant difference was found in the occurrence of DH in areas with differing levels of wheat consumption or iodine intake.
Treatment of parapsoriasis en plaques, mycosis fungoides, and Sézary's syndrome with trioxsalen baths followed by ultraviolet light
Authors: Fischer T , Skogh M.
Published: July 2023

Three patients with parapsoriasis en plaques (PEP), fifteen with mycosis fungoides (MF), and one with S√©zary's syndrome (SS) were given a bath to which a small amount of trioxsalen solution had been added, and then exposed to ultraviolet (UV) radiation from a bank of dysprosium lamps. Within 2--6 months of this treatment the skin lesions healed completely or almost completely in all 3 patients with PEP, in all 7 with MF stage II and in 4 of 5 with MF stage III. Two patients with MF stages IV--V showed a fair degree of improvement. One with erythrodermic form of MF responded, but poorly. The patient with SS and the one with erythrodermic MF responded with severe phototoxic reactions.
The hair in acrodermatitis interopathica--a disease indicator?
Authors: Dupré A , Bonafé JL , Carriere JP.
Published: July 2023

Abstract is not available.
HLA antigens in patients with lichen planus
Authors: Veien NK , Risum G , Jørgensen HP , Svejgaard A.
Published: July 2023

HLA-ABC antigens were determined in 89 patients with biopsy-confirmed lichen planus, and the HLA antigen frequencies were compared with those in 1967 controls. The younger patients were predominantly male, the older patients predominantly female. No significant association was found between HLA types and lichen planus in this study. A slightly greater incidence of HLA-A3 and B5 antigens was seen, but this increased frequency was not as pronounced as reported by others. When combined, available data on HLA and lichen planus indicate a slightly, but significantly, increased frequency of HLA-A3. None of the HLA antigens known to be associated with insulin-dependent diabetes are associated with lichen planus.
Oral photochemotherapy in lichen planus (LP) and mycosis fungoides (MF): ultrastructural modifications of the infiltrating cells
Authors: Ortonne JP , Schmitt D , Alario A , Thivolet J.
Published: July 2023

Six patients (5 with mycosis fungoides and 1 with lichen planus) treated with PUVA, were subjected to biopsy of lesional skin before and during oral photochemotherapy. Ultrastructurally, a reduction in the density of cellular infiltrate was observed in the superficial dermis. In the same areas, necrotic cellular changes were observed. PUVA therapy exercises its beneficial effect by direct destruction of these cells.
Studies on fibronectin in the skin. III. Indirect immunofluorescence studies in lichen planus
Authors: Fyrand O.
Published: July 2023

Fibronectin is a glycoprotein which is responsible for a varity of functions in the human organism, such as mediation of contact between cells and between cells and fibres, opsonic qualities, interaction in the stabilization of fibrin, etc. Fibronectin is an important constituent of the ground substance having a special affinity to collagen. In indirect immunofluorescence studies its presence has been abundantly demonstrated in normal human skin, in collagen-rich structures such as the basement membranes, the papillary and reticular dermis, and in the vascular and neural structures, demonstrable by its characteristic staining patterns. Fibronectin is not found in the epidermis. In lichen planus, the distribution in unaffected skin is identical with that in normal skin, whereas
Erythemal and pigmentary phototest reactions in polymorphic light eruptions
Authors: Jansén CT.
Published: July 2023

The erythemal and pigmentary skin reactions in 110 patients with chronic polymorphic light eruptions (PMLE) and in 154 control persons were investigated by phototesting with a medium pressure mercury lamp. Recording the erythemal threshold (MED) at 2-h intervals for up to 24 h after irradition disclosed no differences between PMLE patients and controls. There was, however, a tendency for the erythemal reaction to subside more slowly in the PMLE patients; this tendency reached a statistically very significant level by day 7. Seventy-three per cent of the PMLE patients and 86% of the controls showed immediate pigmentation. Three days after irradiation, however, 91% of the PMLE patients showed a delayed pigmentary response, in contrast to 66% of the controls; the depth of pigmentation was also greater in the PMLE patients. This study demonstrates that although PMLE patients and healthy controls, taken as groups, may show some differences in their erythemal and pigmentary response, the measurement of the these reactions is of no immediate assistance in the diagnosis of individual cases of polymorphic light eruptions.
Local photochemotherapy in nodular prurigo
Authors: Väätäinen N , Hannuksela M , Karvonen J.
Published: July 2023

Fifteen patients with nodular prurigo were treated with a trioxsalen bath (50 mg/150 l of water) and UVA given in an ordinary PUVA cabin. Good results were observed in 8 and moderate in 7 patients in the initial phase. The result was good or excellent in 13 of the 15 patients during maintenance treatment. The good results are promising in the treatment of this chronic and otherwise therapy-resistant disease.
Pain control in the surgical debridement of leg ulcers by the use of a topical lidocaine--prilocaine cream, EMLA.
Authors: Holm J , Andrén B , Grafford K
Published: July 2023

The analgesic effect of EMLA 5% cream for surgical cleansing of leg ulcers was investigated in an open study and in a double-blind comparison with placebo. Eighty patients with ulcers of venous or arterial origin participated. The cream was applied under occlusion and removed before cleansing. Plasma concentrations of lidocaine and prilocaine were assessed. The maximum individual concentrations were 0.8 microgram/ml for lidocaine and 0.08 microgram/ml for prilocaine. Pain was assessed according to a verbal rating scale and on a 100 mm visual analogue scale. The median VAS pain scores for EMLA and placebo were 18.5 and 84 mm (p less than 0.01). There were no severe adverse reactions. The results show that there is a need for pain control in surgical debridement of leg ulcers and that EMLA cream gives satisfactory analgesia for this procedure.
Furuncular myiasis.
Authors: Reunala T , Laine L J , Saksela O , Pitkänen T , Lounatmaa K
Published: July 2023

Two cases of cutaneous myiasis are described. The first, acquired in West Africa, consisting of 36 furuncle-like lesions, was caused by the larvae of Cordylobia anthropophaga. An adult fly was raised from one larva. In the second patient, the infestation was caused by five larvae of Dermatobia hominis acquired in Central America. Neither patient was aware of any infestation, but the clinical presentation, although also suggestive of bacterial skin infection, showed a typical course and symptoms of cutaneous myiasis in both patients.
Treatment of seborrhoeic dermatitis of the scalp with ketoconazole shampoo. A double-blind study.
Authors: Faergemann J
Published: July 2023

Thirty-six patients with seborrhoeic dermatitis of the scalp and culture positive for Pityrosporum ovale were treated in a double-blind placebo controlled study with ketoconazole shampoo twice weekly for 4 weeks. In the ketoconazole group, 16 of 18 patients (89%) became free of lesions or improved, compared with only 8 of 18 (p less than 0.01) in the placebo group. The patients found the shampoo effective, easy to use and cosmetically attractive.
Plasma concentrations and analgesic effect of EMLA (lidocaine/prilocaine) cream for the cleansing of leg ulcers.
Authors: Enander Malmros I , Nilsen T , Lillieborg S
Published: July 2023

Plasma concentrations of lidocaine and prilocaine were assessed in 8 patients after the application of 8-10 g EMLA 2% cream for 60 min to leg ulcers measuring 31-80 cm2. Maximum individual plasma concentrations were 205 ng/ml for lidocaine and 79 ng/ml for prilocaine, which is twenty times lower than those associated with toxicity. The analgesic effect of EMLA 2% and 5% cream for the surgical cleansing of leg ulcers was compared in a double-blind, four-period, cross-over study in 10 patients. The ulcer was covered with a thick layer of cream for 30 min before four consecutive debridements 1-4 days apart. While the 2% and 5% creams had similar analgesic effects post-cleansing pain tended to be more frequent with the 2% cream.
Nickel in intercellular fluid. Comparison between nickel-allergic patients and controls.
Authors: Bonde I , Beck H I , Jørgensen P J , Grandjean P , Brandrup F
Published: July 2023

No abstract available.
In vitro effects of testosterone, dihydrotestosterone and estradiol on cell growth of human hair bulb papilla cells and hair root sheath fibroblasts.
Authors: Arai A , Von Hintzenstern J , Kiesewetter F , Schell H , Hornstein O P
Published: July 2023

The influence of testosterone, dihydrotestosterone (each 10 ng/ml up to 300 ng/ml) and estradiol (0.2 ng/ml up to 10 ng/ml) on the growth behaviour (cell count, [3H]thymidine uptake, cell doubling time) of subcultured human hair bulb papilla cells and hair root sheath fibroblasts was studied. Papilla cells and root sheath fibroblasts were isolated by microdissection from the same anagen hair follicles obtained from biopsies of androgen-sensitive scalp regions in 6 healthy male subjects. Dihydrotestosterone and testosterone concentrations above 30 ng/ml significantly reduced the growth of both cell types; lower doses had no effect. Estradiol had no distinct influence on the growth curves of either cell type up to 10 ng/ml, whereas higher concentrations significantly increased the growth of both cell types as shown by [3H]thymidine uptake. Papilla cells reacted more sensitively than root sheath fibroblast to dihydrotestosterone and testosterone, as shown by the growth curves, [3H]thymidine uptake, and cell doubling time.
Psoriasis and polyneuropathy. Three case histories.
Authors: Sindrup S H , Ibsen H H , Sindrup J H , Sindrup E H
Published: July 2023

Neurophysiological examination of 3 psoriasis patients with symptoms of polyneuropathy revealed varying degrees of both sensory and motor nerve affection and indicated nerve fibre loss as well as demyelination. Previous reports have suggested a connection between peripheral nerves and psoriasis.
Proliferative Potential of Adipose Tissue in Type 2 Diabetes Mellitus and Superinvasive Opisthorchiasis
Authors:   Tyumen State Medical University, Uruzbayev Rinat M. ,   Multidiscipline Clinical Medical Center “Medical City”, Vikhareva Larisa V. ,   Tyumen State Medical University, Kopylova Yuliya Yu. ,   Medical Examiner’s Office, Manakova Tat’yana A. ,   Multidiscipline Clinical Medical Center “Medical City”, Lebedev Nikita V. ,   Multidiscipline Clinical Medical Center “Medical City”
Published: July 2023

INTRODUCTION: The article presents the results of studying the proliferative activity of adipocytes and of neoangiogenesis in adipose tissue in healthy individuals, in those who died with type 2 diabetes mellitus and with superinvasive opisthorchiasis. The parasite is the original factor that triggers productive processes due to the mutation of numerous proliferative genes. Stem cells are initiated with their subsequent differentiation along the lines of various differons. AIM: to identify proliferative processes and processes of neoangiogenesis of adipose tissue in type 2 diabetes mellitus and superinvasive opisthorchiasis. MATERIALS AND METHODS: sectional material (n = 58): group 1 — healthy individuals (n = 10), group 2 — deceased with type 2 diabetes mellitus from accidental causes (n = 22), group 3 — persons with superinvasive opisthorchiasis died by violence, with duration of invasion more than 10 years (n = 26). The material was subjected to routine histological examination, including histochemical and immunohistochemical staining methods. Further, microscopy was carried out with subsequent statistical analysis. RESULTS: reduction of the proliferative activity of adipose tissue cells in type 2 diabetes mellitus and increase in the parameters in opisthorchiasis invasion compared with healthy individuals were revealed; in diabetes mellitus, the hypertrophic variant of adipose tissue prevailed, the volumetric density of blood vessels decreased, while in patients with superinvasive opisthorchiasis, lipolysis and angiogenesis increased. CONCLUSION: predictors of type 2 diabetes mellitus were assumed to be low proliferative activity of adipocytes, low volumetric density of blood vessels and hypertrophic variant of adipose tissue. With high mitotic activity and hyperplastic variant of adipose tissue, like in superinvasive opisthorchiasis, a protective effect is formed, which can be considered in the context of a predictor factor of type 2 diabetes mellitus.
Microbiocenosis of Skin in Military Personnel with Abscessed Furuncle of the Maxillofacial Region
Authors:   Kirov Military Medical Academy, Petrova Tat’yana V. , Maksyuta Dmitriy A. ,   Kirov Military Medical Academy, Slivkin Anton A. ,   Kirov Military Medical Academy
Published: July 2023

INTRODUCTION: Among non-odontogenic inflammatory diseases of the maxillofacial region, most common are abscessed furuncles. The causative agent of the disease is S. aureus (95%) mostly occurring as a monoculture. AIM: To study the microbial flora of facial skin in military personnel with abscessed furuncle of the maxillofacial region. MATERIALS AND METHODS: In 53 patients with abscessed facial furuncles (the main group) aged from 18 to 25 years, material was taken for bacteriological examination from healthy areas of the skin and from the furuncle with a sterile cotton swab. As a comparison group, a group of 30 practically healthy individuals of the same age was recruited, who also underwent a microbiological examination of facial skin areas. Examinations were performed in the bacteriological laboratory of Kirov Military Medical Academy. RESULTS: Among the representatives of the microflora of healthy skin areas in patients with abscessed furuncles of the face, S. epidermidis was detected in 58% of cases, S. saprophyticus in 33%, S. haemoliticus in 10%. In 30% of admitted patients, Propionibacterium acnes were found. In 4% of cases, S. aureus was detected by us. Fungi of the genus Candida were found in 5% of patients. The microbial composition of the wound discharge in facial furunculosis in 87% of cases was represented by pathogenic S. aureus as a monoculture, in 4% in association with S. saprophyiticus, in 3% with Pseudomonas aeruginoza, in 1% in association with Klebsiella oxytoca, in 5% by S. epidermidis as a monoculture. The species composition of the microflora of the facial skin of healthy people was represented by non-pathogenic staphylococci S. epidermidis (88.1%), S. saprophyticus (40%). In 25.3% of cases, streptococci were also detected. In 10% of cases, yeast-like fungi of Malassezia genus and in 12% of Candida genus were found. In 4% of cases, the pathogenic form of S. aureus was identified. CONCLUSION: The revealed differences in the composition of microorganisms of healthy skin areas between healthy individuals and patients with abscessed facial furuncles permitted to suggest the presence of dysbacteriosis of skin of the maxillofacial region in patients with abscessed furuncles, which led to the development of the disease.
Some Features of Laboratory Parameters in Patients with SARS-CoV-2 with Underlying Overweight and Obesity
Authors:   Vernadsky Crimean Federal University, Beloglazov Vladimir A. ,   Semashko Republican Clinical Hospital, Yatskov Igor’ A. ,   Vernadsky Crimean Federal University, Klimchuk Anastasiya V. ,   Vernadsky Crimean Federal University, Bubley Konstantin V. ,   Vernadsky Crimean Federal University
Published: July 2023

INTRODUCTION: The data of SARS-CoV-2 pandemic studies indicate a pronounced influence of overweight and obesity on the course of the new coronavirus infection and the risk of lethal complications. In this regard, of particular interest are the changes in the parameters of the main routine diagnostic complex identified in patients with SARS-CoV-2 infection having excess body weight and obesity, and also the search for pathophysiological relationships between these changes and the excess body weight. AIM: To evaluate the changes in the parameters of systemic inflammation and coagulogram, as well as of the peripheral blood leukocyte count in patients with COVID-19 with excess body weight and obesity. MATERIALS AND METHODS: 73 patients with PCR-positive result for SARS-CoV-2 in the age group of 44–65 years old admitted to the infectious diseases department of Semashko Republican Clinical Hospital were examined. The patients were divided into 3 clinical groups depending on body mass index (BMI): Group 1 — patients with BMI < 25 kg/m2, Group 2 — patients with excessive body weight, and Group 3 — patients with 1st degree obesity. On admission to the hospital, the patients underwent the general blood analysis, analysis of C-reactive protein (CRP) level, erythrocyte sedimentation rate (ESR), ferritin, coagulogram parameters, as well as a computer tomography (CT) of the chest organs. RESULTS: All the patients with new coronavirus infection had significantly higher parameters of systemic inflammation (CRP and ferritin) compared to the control group (p < 0.05). The group of patients with 1st degree obesity recorded the highest absolute numbers of band and segmented neutrophils, ferritin, and D-dimer (р1–3 < 0.05; р2–3 < 0.05). The levels of ESR, CRP, and fibrinogen were significantly higher in the overweight and obese groups, but no significant differences between groups 2 and 3 were found in the intergroup comparisons (р1–2 < 0.05; р1–3 < 0.05; р2–3 > 0.05). The values of activated partial tromboplastin time and thrombin time did not differ significantly between the clinical groups (p > 0.05). It is also worth noting that the percentage of pulmonary tissue lesions according to CT data varied significantly depending on BMI, with the highest rate in the group of patients with 1st degree obesity (p < 0.05). CONCLUSIONS: Obese patients with COVID-19 have higher level of inflammation and a high risk of thromboembolic complications, which requires more careful monitoring of this category of patients during the course of the disease and in the post-COVID period.
Possibilities of Pathogenetic Therapy in Patients with Functional Pathology of the Digestive System
Authors:   Ryazan State Medical University, Butov Mikhail A. , Shebbi Raniya ,   Ryazan State Medical University, Zhestkova Tat’yana V. ,   Ryazan State Medical University
Published: July 2023

INTRODUCTION: Functional diseases of the gastrointestinal tract presents a real problem to public health. The basis of effective therapy for irritable bowel syndrome (IBS) and functional dyspepsia (FD) is a combined use of symptom-reducing drugs. AIM: To assess the dynamics of the intensity of abdominal pain and changes in the motility of stomach and intestine after treatment of patients with IBS, and with IBS in combination with FD, with STW 5 preparation. MATERIALS AND METHODS: 28 patients with IBS, including patients with a combination of IBS and FD, were taking STW 5 orally, 20 drops 3 times daily before meals for 14 days, to relieve clinical symptoms. Before and after treatment, patients completed a survey using VAS and ‘7 × 7’ questionnaires and underwent peripheral electrogastroenterocolography (EGECG). RESULTS: The level of abdominal pain before treatment in patients with predominating constipation corresponded to a moderate degree of intensity, in patients with diarrhea to a mild degree of intensity. During treatment, 32.1% of patients noted relief of abdominal pain in the second week of treatment; bloating was less common and/or did not occur in 71.4% of patients; 42.9% of study participants noted normalization of stool frequency and/or stool shape. Improvement of clinical condition in terms of FD symptoms was observed in 76.9% of patients with IBS and FD. The use of STW 5 was associated with a 51.3% reduction of pain intensity (p < 0.001) after a week of treatment. Mean changes in the colon coefficient rhythmicity (Crhythm) according to the results of EGECG after treatment in patients with constipation reliably differed from the level of change in Crhythm in patients with diarrhea (p = 0.047). CONCLUSION: The results of the study confirm the effectiveness of STW 5 in the treatment of patients with IBS, and with IBS in combination with FD.
Systemic Analysis of Clinical, Neurophysiological and Psychometric Features of Patients with Pharmacoresistant Epilepsy
Authors:   Ryazan State Medical University, Elmi Oussema , Zhadnov Vladimir A. ,   Ryazan State Medical University, Zorin Roman A. ,   Ryazan State Medical University, Evdokimova Ol’ga V. ,   Ryazan State Medical University, Burshinov Aleksandr O. ,   Ryazan State Medical University, Leonov Gennadiy A. ,   Ryazan State Medical University
Published: July 2023

INTRODUCTION: Drug-resistant epilepsy (DRE) is associated with a high incidence of comorbidities, psychiatric comorbidity, and early mortality in patients, which permits to consider epilepsy a systemic dysfunction of the body as a whole. AIM: Was to describe the features and relationships of clinical, neurophysiological and psychometric parameters of patients with DRE from a systemic standpoint. MATERIALS AND METHODS: A comprehensive clinical and neurophysiological study was conducted in 60 patients diagnosed with epilepsy, who were divided into 2 groups: 30 patients with DRE and 30 patients with remission of seizures. A clinical study was carried out including electroencephalography (EEG) with the calculation of the spectral power of the α-rhythm, the coherence coefficient and the cross-correlation coefficient for each pair of leads. The psychometric study included Eysenck, Strelau tests, the Taylor Anxiety Scale, the Freiburg Personality Inventory (FPI), the Toronto Alexithymia Scale, and the Self-Destructive Tendency Test. An analysis of correlations between clinical, psychometric and neurophysiological parameters in the initial state and with functional load was carried out. Based on the data obtained, graphical models were constructed. RESULTS: In patients with DRE, the clinical features of the disease, consisting in a longer duration of the disease and higher frequency of attacks, negatively correlated with the parameters of the spectral power of the α-rhythm, and with the levels of neuroticism and self-destructive behavior, and the low values of these characteristics can be associated with an unfavorable course of the disease. Rigid, systemic organization of psychological functions in patients with DRE, deficit of inhibitory processes in the nervous system were manifested in numerous correlations between psychometric parameters that negatively affect the quality of life, and also of psychometric parameters and spectral power of the EEG α-rhythm in different functional conditions. Numerous correlations between parameters may evidence a high level of conjugation of neurophysiological mechanisms with parameters in patients with DRE. CONCLUSION: Clinical, psychological and neurophysiological characteristics of patients with DRE are a reflection of a systemic phenomenon consisting in the mobilization of resources of the motivational-emotional, physiological spheres of patients not for external activity, but for inadequate defense reactions, which are subjective in nature.
Modern Method of Surgical Treatment of Stricture of the Parotid Salivary Gland Duct
Authors:   I. P. Pavlov First Saint Petersburg State Medical University, Razumova Aleksandra Ya. , Petrov Nikolay L. ,   I. P. Pavlov First Saint Petersburg State Medical University, Yaremenko Andrey I. ,   I. P. Pavlov First Saint Petersburg State Medical University, Kutukova Svetlana I. ,   I. P. Pavlov First Saint Petersburg State Medical University
Published: July 2023

INTRODUCTION: A modern principle of treatment for obstructive pathologies of salivary glands is saving the gland itself and its functions using minimally invasive methods. This article describes a clinical case of elimination of the stricture of the parotid salivary gland duct and compensation for its expanded section behind the gland with intraoperative visualization using ultrasound. Under US control, the expanded portion was punctured from external access; a conductor was inserted, which passed into the oral cavity through the natural orifice. Through the formed opening, a catheter was installed, which expanded the narrowed area and prevented the reappearance of the stricture. Control examinations in 1, 6 and 12 months showed the functioning of the orifice with release of clear saliva in the satisfactory amount. The patient noted absence of enlargement of the parotid salivary gland and absence of pain at rest and in chewing. Control US in 6 and 12 months showed absence of any signs of stricture and reduction of the orifice diameter to 2 mm. CONCLUSION: The developed method of elimination of stricture of the parotid salivary gland duct can lead to reduction of duration and the scope of surgical intervention, of the risk of re-stricture, postoperative and rehabilitation periods, frequency of using costly equipment, which makes the procedure available.