bluuebunny/crossref_metadata_2025 huggingface dataset:
CAR T-Cell Therapy: Hope and Healing in the Battle Against Cancer
Authors: Paliwal Lata , Ambwani Sonu
Published: November 2024

With numerous forms and a myriad of genetic and environmental factors, cancer continues to be a leading cause of morbidity and mortality worldwide. Advances in cancer research have led to a deeper understanding of the molecular mechanisms driving cancer progression, facilitating the development of targeted therapies and immunotherapies. Chimeric Antigen Receptor T-cell therapy, commonly known as CAR T-cell therapy, represents a groundbreaking approach in the field of cancer immunotherapy. This innovative treatment involves genetically engineering a patient's T cells to express chimeric antigen receptors (CARs) that target specific cancer cells. CAR-T therapy has demonstrated remarkable success in the treatment of certain hematologic malignancies, particularly B-cell malignancies like acute lymphoblastic leukaemia (ALL), chronic lymphocytic leukaemia (CLL), and non-Hodgkin lymphoma (NHL). CAR T-cell therapy also has challenges, such as managing side effects like cytokine release syndrome and neurotoxicity, high treatment costs, and the need for further research to broaden its applicability to solid tumours. CAR T-cell therapies, such as Kymriah and Yescarta, have demonstrated impressive clinical outcomes in patients with relapsed or refractory cancers, offering hope for those with limited treatment options. Additionally, the long-term durability of responses requires continuous monitoring and research.
Impact of Different Organic Nutrient Sources on Soil Chemical Properties in Rabi-season Rapeseed Cultivation
Authors: Lhamu Sonam , Paul Akash , Boruah Anasuya , Yedi Nilo , Alam Sahidul , Kumar Mayank , Zimik Yirmeila V.
Published: November 2024

Aims: The aim of the experiment was to check the effect of various organic soil amendments on the soil chemical properties.
Study Design: The design of the experiment was RandomizedBlock Design (RBD).
Place and Duration of Study: Instructional-cum Research Farm, Department of Agronomy, Assam Agricultural University, Jorhat, Assam, India, during the rabi season of 2018-19.
Methodology: A total of 11 treatments were considered viz.,Control (T0), Farmyard manure @ 10 t/ha (T1), Vermicompost @ 5 t/ha (T2), Poultry Manure @ 5 t/ha (T3), Farmyard manure @ 5 t/ha + Vermicompost @ 2.5 t/ha (T4), Farmyard manure @ 5 t/ha + Poultry Manure @ 2.5 t/ha (T5), Vermicompost @ 2.5 t/ha + Poultry Manure @ 2.5 t/ha (T6), Farmyard manure @ 5 t/ha + Vermicompost @ 2.5 t/ha + Mustard Oil Cake @ 1 t/ha (T7), Farmyard manure @ 5 t/ha + Poultry Manure @ 2.5 t/ha + Mustard Oil Cake @ 1 t/ha (T8), Vermicompost @ 2.5 t/ha + Poultry Manure @ 2.5 t/ha + Mustard Oil Cake @ 1 t/ha (T9), and Farmyard manure @ 5 t/ha + Vermicompost @ 1.25 t/ha + Poultry Manure @ 1.25 t/ha + Mustard Oil Cake @ 1 t/ha (T10).
Results: Results revealed significantly higher levels of organic carbon, available nitrogen (N) and phosphorus (P₂O₅) in the soil after crop harvest following the application of different organic nutrient sources compared to the control.
Conclusion: The application of various organic amendments increased the nutrient uptake by the soil. Organic inputs like Farmyard manure, vermicompost, poultry manure, and their combinations improved soil moisture retention, boosted organic carbon content, and resulted in higher levels of available nitrogen and phosphorus in the soil after harvest compared to the control plot.
Commensal and Pathogenic Bacteria Associated with Farmed Rainbow Trout (Oncorhynchus mykiss Walbum) and Aquaculture Environment
Authors: Lalitha K.V , Murugadas V. , Joseph Toms C.
Published: November 2024

The bacterial microflora associated with farmed rainbow trout (Oncorhynchus mykiss Walbaum) and its aquaculture environment was characterized. The counts of commensal, indicator and pathogenic bacteria were determined and bacterial colonies were characterized. The mean mesophilic counts were 4.5 log CFU g -1 and 7.6 log CFU g -1 in skin with muscle and intestine respectively. Significantly high densities of faecal coliform, E. coli and Enterococci was detected in pond sediment, fish intestine and feed when compared to pond water and trout tissues. A total of 110 randomly selected isolates from TSA were identified and characterized. Of the mesophiles, 70–75% were aerobic Gram-negative bacteria, both motile and non-motile, and members of the Enterobacteriaceae family. The skin microflora of trout was dominated by Gram-negative aerobic rods belonging to Enterobacteriaceae, Aeromonadaceae, Pseudomonas/ Shewanella, Moraxella, Acinetobacter and Flavobacterium. Psychrotrophic bacterial isolates of the genera Pseudomonas, Morganella, Hafnia were identified in trout. Pathogenic bacteria such as Clostridium botulinum, Yersinia enterocolitica could not be detected. However, Aeromonas hydrophila, A. veronii biovar sobria, A. veronii biovar veronii were prevalent in farmed trout. Presence of bacterial pathogens in the trout farm envisages a strict hygienic handling and processing of fish from such culture systems for ensuring public health safety.
Runge-Kutta Finite Element Method for the Fractional Stochastic Wave Equation
Authors: Chukwuyem Nwankwo Jude , Nkonyeasua Njoseh Ignatius , Apanapudor Joshua Sarduana
Published: November 2024

This paper presents the development and application of the Runge-Kutta Finite Element Method (RK-FEM) to solve fractional stochastic wave equations. Fractional differential equations (FDEs) play a significant role in modelling complex systems with memory and hereditary properties, while the inclusion of stochastic components accounts for randomness inherent in physical systems. The fractional stochastic wave equation represents a natural extension of classical wave equations, incorporating both fractional time derivatives and stochastic processes to model phenomena such as anomalous diffusion and noise-driven wave propagation. We propose a hybrid numerical scheme that combines the high accuracy of the Runge-Kutta Method or temporal discretization with the flexibility of the Finite Element Method (FEM) for spatial discretization. The Caputo fractional derivative is used to describe the time-fractional component of the equation. A white noise-driven stochastic term is incorporated into the system to account for randomness. We analyze the stability and convergence properties of the RK-FEM scheme and demonstrate its effectiveness through numerical simulations. The results illustrate that the proposed method provides accurate and stable solutions for fractional stochastic wave equations, making it a robust tool for investigating wave phenomena in complex and uncertain environments.
Periodontitis as Significant Comorbiditiy in Chronic Kidney Disease: A Cross Sectional Study
Authors: Singh Sachin , Narain Upma , Gupta Arvind , Singh Bhavna
Published: November 2024

Introduction: Chronic Kidney Disease (CKD) is a progressive disorder associated with the gradual loss of kidney function over time. It is a significant public health concern worldwide and has been linked to a range of comorbidities, including periodontitis. Periodontitis is a common oral disease characterized by an inflammatory response to bacteria living in dental plaque. This review reveals the evidence for the association between periodontitis and CKD.
Materials and Methods: A prospective study that was conducted in department of medicine at MLN Medical College India from July 2021 to June 2022 in which 50 chronic kidneys patient were included on the basis of inclusion and exclusion criteria. Blood samples were taken and sent for serum interleukin-6, serum procalcitonin, serum vitamin D,CRP, serum creatinine and other relevant investigations. Clinical periodontal examination was performed and classified it by using CDC-AAP classification.
Results: Out of 50 Chronic kidney disease patients, 80% patients presented with periodontitis changes which was considered as cases while 20% patients did not show any sign of periodontitis and was considered as control. In cases percentage of severe periodontitis was found more. We also observed that increased severity of periodontitis was more associated with higher stages of CKD and that was statistically significant (p value -0.012). Inflammatory markers were found statistically significant with pocket depth of periodontitis in cases with respect to control but only serum vitamin d was statistically significant with clinical loss of attachment.
Conclusion: Therefore present review showed that maximum number of CKD patients had periodontitis changes and these changes which were showed by pocket depth and clinical loss of attachment were directly associated with increased inflammatory markers and they indirectly affect the prognosis of CKD patient and management.
Photobiomodulation as an Adjunctive Therapy for Oral Trauma: A Case Report
Authors: Wagmann Daniela Anat Amzallag , San-Martín Magdalena , Cirisola Rolph Wilhem Consolandich , Bruno Maria Laura Hermida , Motta Lara Jansiski , Bussadori Sandra Kalil , Fernandes Kristianne Porta Santos , Horliana Anna Carolina Ratto Tempestini , Mesquita-Ferrari Raquel Agnelli
Published: November 2024

Introduction: Photobiomodulation has been reported to accelerate healing of tissues, promote the formation of new blood vessels, reduce inflammation, and manage pain, which can be highly beneficial when treating trauma patients. There is a lack of controlled clinical studies on the use of PBM for dental trauma, likely due to challenges in standardizing the types of trauma for inclusion and treatment, as well as the complexities introduced by the urgent nature of emergency care in clinical practice. Aim: In light of this challenge, the objective of this case report was to evaluate the effects of PBM in tissue damage resulting from a 7-years-old girl trauma due to a fall incident causing a lip injury (contusion and laceration) and a nasal spine bone fracture. Methods: the PBM with a Diode Laser was used aa an adjuvant with a detailed medication regimen. Red light (660 nm, 3J) was applied for 30 seconds at four points on the upper lip, while infrared light (808 nm, 3J) was applied for 30 seconds at two points near tooth 11 and the nasal spine to cover the affected area. It was performed 5 sessions (initial session at Day 1 of trauma, at 24 hours, 48 hours, one week and three weeks). The upper lip and nasal spine regions were submitted to PBM. Results: Complete absorption of the lip hematoma and healing of mucosal abrasion occurred within a few days. The patient remained pain-free throughout the initial week and thereafter, with no pain medication. The radiographic and clinical assessment indicated no dental damage occurred. Follow-up was for seven months, and no subsequent complications were observed. Conclusion: In this case report, PBM was used as an adjunctive treatment for orofacial trauma, demonstrating potential benefits in reducing pain and tissue inflammation. However, further well-designed clinical trials are needed to confirm its efficacy and establish standardized protocols.
Evaluation of Antiarthritic Protective Effect of Erlangea tomentosa (Oliv. & Hiern) S. Moore (Asteraceae) Methanol Leaf Extract in Complete Freund’s Adjuvant-Induced Arthritis in Rats
Authors: Mboneye Anselme , Onchweri Albert Nyanchoka , Neeza Timothy , Odoma Saidi
Published: November 2024

Objective: Given the rising interest in plant-based therapies for chronic inflammatory diseases like arthritis, this study aimed to explore the potential of Erlangea tomentosa, a plant known for its medicinal properties, as an anti-arthritic agent
Methodology: The plant extract was administered to arthritic rats that had been injected with the Complete Freund’s adjuvant (CFA). The physical anti-arthritis effects of the plant were evaluated using body weight, paw diameter, and a clinical scoring approach. The systemic anti-arthritis properties were evaluated using hematological and biochemical markers of arthritis at the conclusion of the study. Paw joints were taken out for histopathological investigation, and the body weight organ indices for gross abnormalities.
Results: It was found that the methanol leaf extract of the plant significantly reduced the arthritis state, which was obvious in clinical arthritis scoring, CFA-injected paw size, body weight, and the preservation of hematological and biochemical indicators. Histopathology and organ indices also confirmed that the plant extract could reduce the pain and inflammation of animals with CFA-induced arthritis.
Conclusion: The authors have concluded that the plant might help with arthritis. Research into a specific proinflammatory mediator, such as interleukins and tumor necrosis factor, would confirm this activity.
Application of BIM in Modeling Concrete Retaining Walls
Authors: Zhengyang Liu
Published: November 2024

During the hydraulic design phase, challenges often arise, such as poor coordination between different disciplines and ineffective information transfer. These issues can lead to errors in the design process and unreasonable structural designs. As the complexity of engineering projects continues to increase, traditional 2D design methods can no longer meet current demands. As a result, Building Information Modeling (BIM) technology has been introduced, shifting the design approach to 3D modeling. This transition has significantly improved design accuracy and construction efficiency. To further optimize this process, a user interface interaction system based on C# language and the Revit API was developed. Through this system, we successfully implemented the 3D automatic modeling of retaining wall components and a reinforcement plugin. These tools have greatly saved time on repetitive tasks for designers, improving work efficiency. At the same time, they have advanced the informatization of hydraulic concrete structure construction, making the construction process more efficient, precise, and controllable. The 3D deepening design can accurately express the geometric characteristics of the structure, enhancing precision and accelerating the informatization development of hydraulic concrete structure construction.
Psychosocial Impacts of COVID-19 Pandemic on West Africans Based in Victoria Australia who Survived the 2014-16 Ebola Epidemic: The Methodological Approach
Authors: Mandoh Sulaiman L , Bwititi Phillip T , Nwose Ezekiel U
Published: November 2024

Background: COVID-19 pandemic caused some obvious psychosocial distress in virtually all aspects and works of life. This has led to massive volumes of research. Yet, concerns for preparedness against future disease outbreak remains. One of such concerns is around mental health care. Therefore, a research agenda is to investigate the psychosocial impact of COVID-19 as perceived by Australian-based West Africans who survived the 2014-2016 Ebola epidemic before coming to Australia.
Objective: The objective of this article is to describe the options of research methods that have been considered.
Method: The description was designed to take a narrative approach. As such, vivid descriptions of potentially applicable research methods are presented.
Outcome: Paradigms as well as concepts of qualitative and quantitative research rationales are discussed. Sequential approach in mixed-method research is also described. Limitations of the different methodological options are highlighted to inform their pros and cons.
Conclusion: The significance of this article is elucidation of methodologies that underpin the selected methods in the research project.
Reconsidering the Forres cross-slab (Sueno’s Stone), part 1
Authors: Loggie Ruth , Campbell-Howes Fiona , Noble Gordon
Published: November 2024

Standing over 6.5m high, the Forres cross-slab (NJ 04655 59533), erroneously known as Sueno’s Stone, is Scotland’s tallest early medieval carved stone monument. Featuring an impressive series of battle scenes on one side of the monument and a large interlace decorated ring-headed cross on the other, the cross-slab has seen repeated attempts to link its creation to known historical events and to date the stone through art-historical parallels. In the 1990s excavations around the base of the slab identified a circular setting of post holes interpreted as a derrick setting built to enable the erection of the stone. New radiocarbon dates from this post-setting provide support for a 9th-century date for the carving. The 9th century was a time of major change in the balance of power of Pictland/Alba and the stone may have been created as a statement of continuing authority by rulers in the north whose control was under considerable threat or as a memorial of imperial pretensions planted at Forres by rulers in the south whose power was growing.
Professor Dame Rosemary Jean Cramp, DBE FSA FBA HonFSAScot
Authors: Breeze David J , Morris Christopher D
Published: November 2024

Rosemary Cramp was the grande dame of British archaeology, a cheerful, friendly person, adored by her students and supportive of them in return. In 1971 she was appointed the first woman professor at Durham University. From 2001 to 2004 she served as President of the Society of Antiquaries of London, the most northerly based person at that time to have held the post. Her academic achievements were recognised by the state through the award of CBE in 1987 and then by her elevation to Dame in 2021, and by the academic world through the award of honorary degrees from Bradford, Cambridge, Cork, Dublin, Durham and Leicester, as well as two Festschriften.
Natural products as new photoprotection strategies
Authors: Godoy Mauren Knorst , Nunes Viviane Cecília Kessler , Bonfanti-Azzolin Gabriela
Published: November 2024

Purpose: One of the great harms to human health comes from excessive exposure and lack of protection to ultraviolet (UV) radiation. The deleterious effects are numerous and require effective protection systems. Recent strategies have been explored with different types of medicinal plants and natural compounds, obtaining significant results for photoptation. The aim of this study was to investigate the photoprotective effects of natural products in in vivo models. Methods: This is an integrative literature review. Results: The compiled data showed that the radiations most used in the studies were UVB radiation and the experimental models in animals stood out from the human models. As for the form of administration, the findings emphasized the topical route, and formulations with extracts were the most used. Conclusion: natural compounds have potential as photoprotection agents, enabling new forms of protection against damage induced by UV radiation.
LESÃO RENAL AGUDA EM PACIENTES TRAUMATIZADOS HOSPITALIZADOS EM UNIDADE DE TERAPIA INTENSIVA
Authors: Lentsck Maicon Henrique , Tomen Karla Bianca , Oliveira Bruna Eduarda Schreiner de , Malaquias Tatiana da Silva Melo , Pelazza Bruno Bordin , Baratieri Tatiane
Published: November 2024

Introdução: A lesão renal aguda (LRA) é uma condição grave e frequente no paciente traumatizado, gera custos, maior tempo de permanência hospitalar e associa-se a elevada morbimortalidade e pior prognóstico. Objetivo: Descrever a incidência e as características clínicas de pacientes com LRA no trauma e a associação de LRA em desfechos clínicos. Métodos: Estudo de coorte retrospectiva, com dados de prontuários de adultos hospitalizados por trauma em unidade de terapia intensiva (UTI). Incluídos pacientes acima de 18 anos, admitidos por lesões, com permanência maior que 48h na UTI e dois resultados de creatinina. Utilizado o RIFLE (Risk, Injury, Failure, Loss, End-Stage) para definir a classificação da LRA e a estatística descritiva e análise bivariada por meio do teste Qui-quadrado de Pearson para as variáveis categóricas. Para a avaliação das medianas dos tempos de internação foi realizado o teste Mann-Whitney. Resultados: Foram analisados 286 indivíduos hospitalizados por trauma em UTI, conforme a classificação RIFLE a LRA afetou 18,5% destes pacientes, predominantemente homens com média de 37,5 anos, tendo grande relevância nos idosos e portadores de diabetes mellitus. Os mais atingidos foram vítimas de trauma contuso, politrauma e acidentes de trânsito, a cabeça foi a parte mais afetada. O óbito e a permanência hospitalar foram fatores que apresentaram associação com a presença de LRA. Conclusão: Esta pesquisa identificou uma taxa relativamente alta de incidência de LRA na população estudada. A associação do óbito com a LRA remete a importância da identificação precoce para manejo adequado.
ASSESSMENT OF THE EFFECT OF COVID-19 ON INDIVIDUALS’ SUBJECTIVE WELLBEING, EXERCISE, SLEEP AND DIET
Authors: Nunes Eryclis , Hermes do Nascimento Fernanda , Carneiro Valim-Rogatto Priscila , Segheto Wellington , Maria de Melo Camila
Published: November 2024

The COVID-19 pandemic changed people’s lives in ways that affected subjective well-being (SWB) and lifestyle. Objective: To evaluate SWB during the COVID-19 pandemic and its effects on physical exercise, sleep and nutrition. 72 men and women ages 20 to 60 were assessed for lifestyle before and during the pandemic, and SWB was evaluated during the pandemic. The negative affect dimension had the highest score (3.77 ± 0.71) among the SWB spheres. The pandemic has reduce in the practice of physical exercise in 61% of the participants who practiced physical exercise before the pandemic (p = 0.001). An increase in total sleep time (TST)  (p = 0.0003) and improvement in sleep quality were observed during the pandemic, and the individuals reported more sleep time said they did so to take care of their health (p = 0.001). Regarding eating habits, 79.12% of the participants considered their diet to be healthy, 34.72% improved their diet and 58.3% increased their appetite. There was a predominance of negative effects in relation to SWB, and these feelings may have been associated with reduced physical exercise. Social distancing resulted in an increase in TST and did not promote changes in eating habits.
PARA QUAIS ROSTOS AS CÂMERAS APONTAM? RESISTÊNCIAS À BANALIZAÇÃO INSTITUCIONAL DO RECONHECIMENTO FACIAL NO BRASIL
Authors: Purificação Melo Paulo Victor
Published: November 2024

Como resistir e se opor à adoção de uma tecnologia que tem sido largamente utilizada e defendida por gestores públicos como essencial à segurança coletiva? Esta inquietação foi a base para o desenvolvimento deste artigo, que analisa ações de mobilização realizadas por três articulações da sociedade civil brasileira que reivindicam o banimento do uso de reconhecimento facial: Tire Meu Rosto Da Sua Mira, #SaiDaMinhaCara e Sem Câmera na Minha Cara. Por meio de levantamento e análise documental e entrevistas semiestruturadas, conclui-se que uma série de iniciativas em comum têm contribuído para a ampliação das discussões sobre a temática. Por outro lado, aponta-se aqui, como desafio principal, a necessidade de diálogo com setores da população que não têm uma presença constante no digital e, vale frisar, estão entre os alvos prioritários das tecnologias de vigilância instaladas nos espaços públicos.
DIREITO ANIMAL E ABORTO: UM DIÁLOGO A PARTIR DO PENSAMENTO DE CAROL ADAMS
Authors: Barbosa Amanda Souza , Gordilho Heron Santana
Published: November 2024

Este artigo analisa os argumentos do movimento ecofeminista que promovem uma aproximação entre os direitos dos animais e dos direitos sexuais e reprodutivos das mulheres, especialmente o direito ao aborto. Inicialmente, o artigo analisa a criminalização da interrupção da gestação no Brasil, para em seguida compreender os contornos do discurso ecofeminista sobre o acesso ao aborto por mulheres. Por fim, analisa os sistemas de dominação masculina que submete dos animais à exploração institucionalizada e criminaliza as mulheres em estado de vulnerabilidade que interrompem uma gestação indesejada. O método de abordagem é o histórico-evolutivo e as técnicas de pesquisa bibliográfica e documental.
AÇÃO CIVIL PÚBLICA - ANIMAIS COMUNITÁRIOS: REMOÇÃO DE CASINHAS DOS CACHORROS DA VIA PÚBLICA
Authors: 3ª Câmara Cível Tribunal de Justiça do Estado do Rio Grande do Sul
Published: November 2024

APELAÇÃO CÍVEL. DIREITO PÚBLICO NÃO ESPECIFICADO. AÇÃO CIVIL PÚBLICA. ANIMAIS COMUNITÁRIOS. REMOÇÃO DE CASINHAS DOS CACHORROS DA VIA PÚBLICA. MUNICÍPIO DE PORTO ALEGRE. OMISSÃO ESTATAL EVIDENCIADA. INTERVENÇÃO JUDICIAL. POSSIBILIDADE EXCEPCIONAL NA GARANTIA DE DIREITOS FUNDAMENTAIS. LEI COMPLEMENTAR MUNICIPAL Nº 694/2012. LEI ESTADUAL Nº 15.254/19.
OS DESAFIOS PARA A SAÚDE PÚBLICA NA ERA DO COLAPSO AMBIENTAL: O VÍRUS OROPOUCHE (OROV) ENQUANTO PATOLOGIA ZOONÓTICA NA REGIÃO DAS AMÉRICAS
Authors: Sturza Janaína Machado , Dutra Gabrielle Scola
Published: November 2024

A temática da presente pesquisa centra-se no vírus Oropouche (OROV) no contexto da saúde pública. O objetivo geral é discutir os desafios para a saúde pública sob a égide da Era do Colapso Ambiental a partir de uma análise do vírus Oropouche enquanto patologia zoonótica no locus das Américas. Os objetivos específicos são: 1) Abordar as implicações das crises ambientais no contexto da saúde pública global a partir da existência de patologias zoonóticas; 2) Analisar o alerta epidemiológico do vírus Oropouche na região das Américas a partir do diagnóstico do vírus e das recomendações feitas pela Organização Pan-Americana da Saúde (OPAS). A pesquisa é arquitetada, metodologicamente, pelo método hipotético-dedutivo, instruído por uma análise bibliográfica e documental. A base teórica escolhida para fundamentar a discussão é a Teoria do Direito Fraterno, desenvolvida pelo jurista italiano Eligio Resta. Diante dos desafios para a saúde pública na Era do Colapso Ambiental, questiona-se: é possível uma análise sanitária sobre o vírus Oropouche na região das Américas, pelo Direito Fraterno? Sob a perspectiva da fraternidade, constata-se que a Era do Colapso Ambiental estimula o surgimento global de patologias zoonóticas e tal acontecimento catastrófico impõe desafios para a saúde pública, à medida em que se faz imprescindível a perfectibilização de pactos fraternos de responsabilidade pela humanidade enquanto lugar comum.
MEDIDA CAUTELAR NA AÇÃO DIRETA DE INCONSTITUCIONALIDADE 7.704 SÃO PAULO
Authors: Min. Flávio Dino Relator
Published: November 2024

Trata-se de ação direta de inconstitucionalidade ajuizada pelaAssociação Brasileira da Indústria de Produtos Para Animais deEstimação e pelo Instituto Pet Brasil contra a Lei Estadual nº 17.972/24,promulgada em 10 de julho de 2024 no Estado de São Paulo
Site‐specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein‐mediated membrane fusion
Authors: Chandra Sunandini , Kumar Manoj , Sharma Nishi R. , Sarkar Debi P.
Published: June 2019

Connivance of cellular factors during virus‐host cell membrane fusion is poorly understood. We have recently shown that cellular villin plays an important role during membrane fusion of reconstituted Sendai virosomes with hepatocytes. Here, we employed villin‐null Chinese Hamster Ovary (CHO) cells, where villin expression led to an increased fusion with virosomes, which was further enhanced due to tyrosine phosphorylation in the presence of c‐src. However, the villin RRI mutant, lacking actin‐severing function, failed to augment membrane fusion. Furthermore, quantitative mass spectrometry and detailed analysis revealed Tyr499 to be the key phosphorylation site of villin responsible for the enhancement of virosome‐CHO cell fusion. Overall, our results demonstrate a critical role for villin and its cell‐type dependent phosphorylation in regulating membrane fusion.
LncRNA FENDRR attenuates adriamycin resistance via suppressing MDR1 expression through sponging HuR and miR‐184 in chronic myelogenous leukaemia cells
Authors: Zhang Feng , Ni Haiwei , Li Xiaoman , Liu Hai , Xi Tao , Zheng Lufeng
Published: June 2019

Chemotherapy is a major anticancer therapeutic modality, however, multidrug resistance (MDR) is frequently observed and hinders treatment efficacy. Here, we investigated the role and potential mechanism of the long noncoding RNA (lncRNA) FENDRR in adriamycin resistance of chronic myeloid leukaemia (CML) cells. FENDRR overexpression attenuates adriamycin resistance, as shown by increased Rhodamine 123 accumulation, promotion of cell apoptosis in vitro and suppression of tumour growth in vivo. Mechanistically, we identified that FENDRR reduces the interaction of the RNA‐binding protein HuR with MDR1 via acting as a sponge, and miR‐184 competitively binds to FENDRR with HuR. Thus, the HuR/FENDRR/miR‐184 interaction contributes to MDR1 activity. These findings indicate that FENDRR is a potential target for reversing adriamycin resistance.
AR splice variants in circulating tumor cells of patients with castration‐resistant prostate cancer: relation with outcome to cabazitaxel
Authors: Sieuwerts Anieta M. , Onstenk Wendy , Kraan Jaco , Beaufort Corine M. , Van Mai , De Laere Bram , Dirix Luc Y. , Hamberg Paul , Beeker Aart , Meulenbeld Hielke J. , Creemers Geert‐Jan , van Weerden Wytske M. , Jenster Guido W. , Nieuweboer Annemieke J. M. , Mathijssen Ron H. J. , de Wit Ronald , Martens John W. M. , Sleijfer Stefan
Published: June 2019

The androgen receptor splice variant (AR‐V) 7 in circulating tumor cells (CTCs) is a predictor for resistance to anti‐AR‐targeted treatment, but not to taxane‐based chemotherapy in metastatic castration‐resistant prostate cancer (mCRPC). In this study, we investigated whether the presence of two constitutively active variants (AR‐V3, AR‐V7) and two other conditionally activated variants (AR‐V1, AR‐V9) vs full‐length androgen receptor (AR‐FL) measured in CTCs from patients with mCRPC were associated with outcome to therapy with the taxane cabazitaxel. Blood was collected at baseline and after two cycles of cabazitaxel from 118 mCRPC patients starting cabazitaxel in a prospective phase II trial. CellSearch‐enriched CTCs were enumerated and in parallel characterized for the presence of the AR‐Vs by reverse transcription quantitative polymerase chain reaction. Correlations with CTC and prostate‐specific antigen response to cabazitaxel as well as associations with overall survival (OS) were investigated. All AR‐Vs were frequently present and co‐expressed at frequencies of 31–48% at baseline and at 19–40% after two cycles of cabazitaxel. No specific directions of change in the measured variants were detected between the start of treatment and after two cycles of cabazitaxel. No associations between the presence of AR‐V3 and AR‐V7 and outcome to cabazitaxel were observed. While a reduction in CTCs to < 5 CTCs during treatment (CTC5‐response) was less often observed in patients with AR‐V9‐positive CTCs at baseline (P = 0.004), the CTC5‐adjusted detection of AR‐V1 after two cycles of cabazitaxel was an independent prognostic factor for OS [HR 2.4 (95% CI 1.1–5.1, P = 0.03)]. These novel findings are expected to contribute to more personalized treatment approaches in mCRPC patients.
S
porichthyaceae
Authors: Trujillo Martha E. , Normand Philippe
Published: June 2019

Spo.ro.tri.cha.ce'ae. N.L. fem. n.
Sporichthya
type genus of the family; suff.
‐aceae
ending to denote a family; N.L. fem. pl. n.
Sporichthyaceae
the
Sporichthya
family.

Actinobacteria / Actinobacteria / Frankiales / Sporichthyaceae

Group of microorganisms with a unique and complex morphology. Aerobic or facultatively anaerobic. Gram‐stain‐variable. Aerial hyphae have a unique upright posture maintained by holdfasts, thickened cells that anchor the erect chains onto the solid surface. Occasionally, chains may be branched.
LL
‐Diaminopimelic acid (
LL
‐DAP) is the diagnostic amino acid of the peptidoglycan. Fatty acid profiles contain iso‐branched, saturated, unsaturated, and 10‐methyl fatty acids in varying amounts. Menaquinone composition is also variable and may include MK‐9(H
6
), MK‐9(H
4
), MK‐9(H
8
), and MK‐8(H
6
). The family is monogeneric, presently harboring the genus
Sporichthya
and phylogenetically related to the families
Frankiaceae
and
Acidothermaceae
.

DNA G + C content (mol%)
: 71.0–71.4 (HPLC, WGS).

Type genus
:
Sporichthya
Lechevalier, Lechevalier and Holbert 1968, 279
AL
.
V
icinamibacteraceae
Authors: Huber Katharina J. , Overmann Jörg
Published: June 2019

Vi.ci.na.mi.bac.te.ra.ce'ae. N.L. masc. n.
Vicinamibacter
, type genus of the family; suff. ‐
aceae
ending to denote a family; N.L. fem. pl. n.
Vicinamibacteraceae
, the
Vicinamibacter
family.

Acidobacteria / ‘Vicinamibacteria’ / ‘Vicinamibacterales’ / Vicinamibacteraceae

The
Vicinamibacteraceae
represent the first described family within the globally widespread
Vicinamibacteria
(=former subdivision 6
Acidobacteria
). 16S rRNA gene analyses clearly delineate the members of
Vicinamibacteraceae
from the other acidobacterial families. The (short) rods stain Gram‐negative, divide by binary fission, do not form capsules or spores, are nonmotile, and occur either as single cells or in tight aggregates. Members of the
Vicinamibacteraceae
are aerobic, neutrophilic, and psychrotolerant to mesophilic chemoorganoheterotrophs that grow on different simple sugars, but particularly well on complex proteinaceous compounds.
Iso
‐C
15:0
, summed feature 3 (C
16
:1
ω7
c
/C16:1 ω6
c
), C
18
:1
ω7
c
or ω9
c
or ω11
c
,
iso
‐C
17:1
ω9
c
, C
16:0
, and
iso
‐C
17:0
are the major fatty acids. MK‐8 is the major respiratory quinone.
Iso
‐diabolic acid and 6‐methyl
iso
‐diabolic acid are present. The major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol. An unidentified glycolipid or unknown phospholipids are also detectable in some cases. The strains have been isolated either from an African semiarid riparian forest soil with a neutral pH or from a mown pasture grassland soil with slightly acidic pH in Germany.

DNA G + C content (mol%)
: 64.7–65.9.

Type genus
:
Vicinamibacter
Huber, Geppert, Wanner, Fösel, Wüst and Overmann 2016, 2976
VP
.
S
porichthya
Authors: Trujillo Martha E. , Normand Philippe
Published: June 2019

Spor.ich'thy.a
. Gr. fem. n.
spora
seed (and in biology, a spore); Gr. masc. n.
ichthys
fish; N.L. fem. n.
Sporichthya
an organism with fish‐like spores.

Actinobacteria / Actinobacteria / Frankiales / Sporichthyaceae / Sporichthya

Aerobic or facultatively anaerobic actinobacteria. Cells are Gram‐stain‐variable, with young cells being Gram‐stain‐negative and older cells gradually acquiring a Gram‐stain‐positive reaction. Aerial hyphae have a unique
upright posture maintained by holdfasts
, thickened cells that anchor the erect chains onto the solid surface. Occasionally, chains may be branched.
Cells in the chain look asymmetrical with constricted separations between cells
. No substrate mycelium is formed. The aerial mycelium forms rod‐shaped to coccoid spores that may develop
polar flagella
when grown in liquid media. The cell wall contains a large amount of
LL
‐DAP. Menaquinone composition is also variable and may include MK‐9(H
6
), MK‐9(H
4
), MK‐9(H
8
), and MK‐8(H
6
). The phospholipid composition may consist of phosphatidyl inositol, phosphatidyl glycerol, and diphosphatidyl glycerol. Mesophilic; grows between 10 and 42°C. Chemoorganotrophic. Complex carbohydrates are utilized. The genus
Sporichthya
belongs to the family
Sporichthyaceae
in the order
Sporichthyales
and is phylogenetically related to the genera
Acidothermus
and
Frankia
. It contains two species,
Sporichthya polymorpha
and
Sporichthya brevicatena
, with
S. polymorpha
being the type species.

DNA G
+
C content (mol%)
: 71.0–71.4 (HPLC, WGS).

Type species
:
Sporichthya polymorpha
Lechevalier, Lechevalier and Holbert 1968, 279
AL
.
Methanolobus
Authors: Lai Mei‐Chin
Published: June 2019

Me.tha.no.lo'bus. N.L. neut. n.methanummethane; Gr. masc. n.loboslobe; N.L. masc. n.Methanolobusmethane (‐producing) lobe.

Euryarchaeota / Methanosarcinales / Methanosarcinales / Methanosarcinaceae / Methanolobus

Species of the genusMethanolobusare strictly anaerobic methane‐producing archaea. Cells are irregular cocci, 0.5–1.5 µm in diameter, sometimes forming loose aggregates, and stain Gram‐negative. Cells are surrounded by a unit membrane and a protein surface layer. Flagella, when present, are monotrichous forMethanolobus tindarius, polar for “Methanolobus psychrophilus,” and multiple forMethanolobus profundi. Optimum temperature, 37°C; maximum, 40–50°C; minimum, 10–15°C; temperature range maximum, 25–50°C; minimum, 0–30°C. pH range, 5.5–9.4. Salt (NaCl) range, 0–2.0 M. The catabolic substrates are methanol, methylamine, dimethylamine, trimethylamine, and dimethylsulfide. No growth on H2/CO2, formate, acetate, or alcohols other than methanol. A member of the familyMethanosarcinaceaeand orderMethanosarcinales.

DNA G + C content (mol%): 39.0–48.3 (Tm).

Type species:Methanolobus tindariusKönig and Stetter 1983, 439VP(Effective publication: König and Stetter 1982, 488).
Jeotgalibaca
Authors: Vela Ana Isabel , Fernández‐Garayábal José F.
Published: June 2019

Je.ot.ga.li.ba′ca. N.L. neut. n.jeotgalum(from Korean n.jeotgal), traditional Korean food; L. fem. n.bacaa grain or berry, and in bacteriology a coccus; N.L. fem. n.Jeotgalibaca, coccus from jeotgal.

Firmicutes / Bacilli / Bacillales / Carnobacteriaceae / Jeotgalibaca

Gram‐positive cocci.Oxidase‐ and catalase‐negative. Cells occur in tetrads, sarcinae, or irregular conglomerates.Non‐spore‐forming. Nonmotile. Facultative anaerobe. Chemoheterotrophic.Peptidoglycan type A4α. Major fatty acids are C16 : 0and C18 : 1ω9c. Polar lipids include phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and several unidentified glycolipids, aminolipids, and phospholipids. Members of the familyCarnobacteriaceae, orderLactobacillales, classBacilli, phylumFirmicutes. Known habitats areseujeot(traditional Korean food) and joint of pigs. The full range of habitat is not known.

DNA G + C content (in silico mol%): 37.5–38.3.

Type species:Jeotgalibaca dankookensisLee, Trujillo, Kang and Ahn 2014, 1734VP.
S
ulfuricella
Authors: Watanabe Tomohiro , Kojima Hisaya
Published: June 2019

Sul.fu.ri.cel'la. L. neut. n.
sulfur
sulfur; L. fem. n.
cella
a small room and in biology, a cell; N.L. fem. n.
Sulfuricella
sulfur‐oxidizing cell.

Proteobacteria / Betaproteobacteria / Sulfuricellales / Sulfuricellaceae / Sulfuricella

Gram‐negative, facultatively anaerobic, straight rods. Motile. Non‐spore‐forming. Kanamycin‐ and ampicillin‐sensitive. Catalase‐negative and oxidase‐positive. Capable of anaerobic growth on nitrate and nitrous oxide as an electron acceptor. Nitrate is reduced to dinitrogen via periplasmic nitrate reductase, nitrite reductase, nitric oxide reductase, and nitrous oxide reductase. Autotrophic growth on thiosulfate and elemental sulfur as an electron donor. The end product of sulfur oxidation is sulfate. The oxidation of thiosulfate to sulfate is catalyzed by multiple proteins, that is, sulfur‐oxidizing enzymes (SoxXYZAB), dissimilatory sulfite reductase (DsrABEFHCMKJOP), adenylylsulfate reductase (AprBA), sulfite‐oxidizing enzymes (SoeABC), and ATP sulfurylase (Sat). Require thiosulfate as a sulfur source for growth. Carbon dioxide is fixed via the Calvin–Benson–Bassham cycle. Neutrophilic, growing optimally between pH 7.5 and 8.0. Grow at temperatures between 0 and 28°C. Prefer low salt content media. The major fatty acid is C
16:1
ω7
c
and/or C
16:1
ω6
c
. Typically found in soils, aquifers, freshwater lakes, and drinking water distribution system.

DNA G + C content (mol%)
: 56.1.

Type species
:
Sulfuricella denitrificans
Kojima and Fukui 2010, 2865
VP
.
U
mboniibacter
Authors: Romanenko Lyudmila A.
Published: June 2019

Um.bo.ni.i.bac'ter. N.L. neut. n.
Umbonium
, scientific name of a genus of marine mollusk; N.L. masc. n.
bacter
, rod; N.L. masc. n.
Umboniibacter
, a rod isolated from
Umbonium costatum
.

Proteobacteria / Gammaproteobacteria / Cellvibrionales / Cellvibrionaceae / Umboniibacter

Gram‐negative, aerobic, oxidase‐positive, catalase‐positive, or catalase‐weakly positive, rod‐shaped bacteria. Chemoorganoheterotrophic. Sodium ions are essential for growth. The predominant isoprenoid quinone is Q‐7. The major polar lipids include phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The minor polar lipid components are an unidentified aminolipid, two unidentified phospholipids, three unidentified aminophospholipids, and four unidentified lipids. The major fatty acids are C
17:1
ω6
c
, C
17:0
, C
16:0
, C
16:1
ω7
c
, and C
13:0
. The known habitat is the marine environment.
Umboniibacter
is a genus in the phylum
Proteobacteria
, family
Cellvibrionaceae
, order
Cellvibrionales
, class
Gammaproteobacteria
.

DNA G + C content (mol%)
: 48.9–51.7 (
T
m
, HPLC).

Type species
:
Umboniibacter marinipuniceus
Romanenko, Tanaka and Frolova 2010, 607
VP
.
State estimation for bilinear systems through minimizing the covariance matrix of the state estimation errors
Authors: Zhang Xiao , Ding Feng , Yang Erfu
Published: June 2019

This paper considers the state estimation problem of bilinear systems in the presence of disturbances. The standard Kalman filter is recognized as the best state estimator for linear systems, but it is not applicable for bilinear systems. It is well known that the extended Kalman filter (EKF) is proposed based on the Taylor expansion to linearize the nonlinear model. In this paper, we show that the EKF method is not suitable for bilinear systems because the linearization method for bilinear systems cannot describe the behavior of the considered system. Therefore, this paper proposes a state filtering method for the single‐input–single‐output bilinear systems by minimizing the covariance matrix of the state estimation errors. Moreover, the state estimation algorithm is extended to multiple‐input–multiple‐output bilinear systems. The performance analysis indicates that the state estimates can track the true states. Finally, the numerical examples illustrate the specific performance of the proposed method.
Spontaneously Regenerative Tough Hydrogels
Authors: Qu Gang , Li Yang , Yu Yafeng , Huang Yuxing , Zhang Wei , Zhang Han , Liu Zhou , Kong Tiantian
Published: June 2019

Sponges, Neofibularia nolitangere, can regenerate spontaneously after being broken down into small pieces, and the regenerated structure maintains the original appearance and function. Synthetic materials with such capabilities are highly desired but hardly achieved. Presented here is a sponge‐inspired self‐regenerative powder from a double‐network (DN) tough hydrogel. Hydrogels are regenerated from their powder form, by addition of water, with preservation of the original appearance and mechanical properties. The powder‐hydrogel‐powder cycle can be repeated multiple times with little loss in mechanical properties, analogous to the regeneration of sponges. These DN hydrogels can be conveniently stored and easily shaped upon regeneration. This work may have implications in the development of regenerative materials for coatings and adhesives.
Dinitrogen‐Molybdenum Complex Induces Dinitrogen Cleavage by One‐Electron Oxidation
Authors: Katayama Akira , Ohta Takehiro , Wasada‐Tsutsui Yuko , Inomata Tomohiko , Ozawa Tomohiro , Ogura Takashi , Masuda Hideki
Published: June 2019

Reported here is the N2cleavage of a one‐electron oxidation reaction usingtrans‐[Mo(depe)2(N2)2] (1) (depe=Et2PCH2CH2PEt2), which is a classical molybdenum(0)‐dinitrogen complex supported by two bidentate phosphine ligands. The molybdenum(IV) terminal nitride complex [Mo(depe)2N][BArf4] (2) (BArf4=B(3,5‐(CF3)2C6H3)4) is synthesized by the one‐electron oxidation of1upon addition of a mild oxidant, [Cp2Fe][BArf4] (Cp=C5H5), and proceeds by N2cleavage from a MoII‐N=N‐MoIIstructure. In addition, the electrochemical oxidation reaction for1also cleaved the N2ligand to give2. The dimeric Mo complex with a bridging N2is detected by in situ resonance Raman and in situ UV‐vis spectroscopies during the electrochemical oxidation reaction for1. Density‐functional theory (DFT) calculations reveal that the unstable monomeric oxidized MoIspecies is converted into2via the dimeric structure involving a zigzag transition state.
Study of the chemical structure and their nematicidal activity of N2O2 tetradentate Schiff base metal complexes
Authors: Abd El‐Hamid S.M. , Sadeek S.A. , Abd El‐Lattif N.S.
Published: June 2019

Coordination compounds of Cu (II), Y (III), Zr (IV) and La (III) with the tetradentate Schiff base (H2L) obtained through the condensation of p‐phenylenediamine with salicylaldehyde under reflux conditions. The complexes were characterized by elemental analysis, magnetic susceptibility, molar conductance and also, with various spectroscopic techniques such as 1H NMR, UV–Vis., IR and XRD techniques. Electrolytic nature of complexes was ascertained by molar conductance values. In these four complexes, the ligand chelates act in a tetradentate manner via azomethine nitrogen and oxygen atoms of phenolic groups. Electronic spectroscopic data are in agreement with an octahedral geometrical structure. Thermal degradation analyses in nitrogen gas were used to investigate the number and location of water molecules. The chemical formulae of metal complexes were confirmed by microanalytical data. The activation thermodynamic parameters, such as, E*, ΔH*, ΔS* and ΔG* were calculated from the DTG curves using Coats Redfern (CR) and Horowitz–Metzeger (HM) methods at n = 1 or n ≠ 1. Nematicidal activities indicate that the ligand exhibit greater activity when compared to its complexes. In addition metal complexes displayed good moderate nematicidal activities.
The Effect of Branched‐Chain Fatty Acid Alkyl Esters on the Cold‐Flow Properties of Biodiesel
Authors: Dunn Robert O. , Wyatt Victor T. , Wagner Karen , Ngo Helen , Hums Megan E.
Published: June 2019

Biodiesel (fatty acid methyl esters [FAME]) is produced from various fats, oils, and greases (FOG) using catalytic transesterification with methanol. These fuels have poor cold‐flow properties depending on the fatty acid (FA) composition of the parent FOG. Improving the cold‐flow properties of biodiesel will enhance its prospects for use during cooler months in moderate temperature climates. This work is a study on the use of skeletally branched‐chain alkyl esters (BCAE) composed of the isopropyl, n‐butyl, and 2‐ethylhexyl esters of iso‐oleic acid isomers (iPr‐iOL, nBu‐iOL, and 2EH‐iOL). These BCAE additives were tested in blends with linear‐FAME (L‐FAME) derived from soybean oil (SME), lard (LME), tallow (TME), and sewage scum grease (SGME). Binary L‐FAME/SME admixtures were also studied. Admixtures were tested for the effects of the additives on cloud point (CP), pour point (PP), and kinematic viscosities at standard (ν40 = 40 °C) and low temperatures (TL) = CP + 5 °C (νL). Although the BCAE additives were more effective than SME, relatively large additive concentrations (yAdd) were needed to depress CP and PP by more than 2 °C. Admixtures with high concentrations of BCAE additive had ν40 > 6.0 mm2 s−1, the maximum limit in ASTM fuel specification D 6751. While the iPr‐iOL and nBu‐iOL additives may be blended at concentrations up to yAdd = 0.50, 2EH‐iOL should not exceed yAdd = 0.28 in LME, 0.31 in SGME, 0.35 in TME, or 0.41 in SME to avoid driving the admixture out of specification. Some anomalies observed in the results at low yAdd for SGME/BCAE admixtures were speculated to have been affected by the low‐temperature rheology of SGME.