bluuebunny/crossref_metadata_2025 huggingface dataset:
“Pets Negotiable”: How Do the Perspectives of Landlords and Property Managers Compare with Those of Younger Tenants with Dogs?
Authors: Graham Taryn , Milaney Katrina , Adams Cindy , Rock Melanie
Published: February 2018

Previous research has shown that housing insecurity contributes to animal relinquishment and that tenants with dogs face disadvantages in the rental market. Still, little is known about how dog owners navigate rental markets, nor how landlords and property managers perceive dogs and other pets. This case study reports on in-depth interviews with younger tenants with dogs and on open-ended survey responses from landlords and property managers. In their housing searches, tenants with dogs reported feeling powerless in negotiations and feeling discriminated against. They described settling for substandard properties, often located in less desirable neighborhoods. Also, some said they felt obliged to stay put in these rentals, given how difficult it had been to find a place that would accommodate their dogs. Meanwhile, landlords and property managers indicated that listings advertised as “pet-friendly” tend to receive more applicants than listings in which pets are prohibited. Suggestions for improvement included meeting pets prior to signing the lease; getting everything in writing; steering clear from furnished units; charging utilities to tenants; and speeding up the pet approval process when dealing with condominium boards. These suggestions offer implications for future research, partnerships, and policy options to improve the prospects of pets and their people in rental housing.
Bacteriophage Interactions with Marine Pathogenic Vibrios: Implications for Phage Therapy
Authors: Kalatzis Panos , Castillo Daniel , Katharios Pantelis , Middelboe Mathias
Published: February 2018

A global distribution in marine, brackish, and freshwater ecosystems, in combination with high abundances and biomass, make vibrios key players in aquatic environments, as well as important pathogens for humans and marine animals. Incidents of Vibrio-associated diseases (vibriosis) in marine aquaculture are being increasingly reported on a global scale, due to the fast growth of the industry over the past few decades years. The administration of antibiotics has been the most commonly applied therapy used to control vibriosis outbreaks, giving rise to concerns about development and spreading of antibiotic-resistant bacteria in the environment. Hence, the idea of using lytic bacteriophages as therapeutic agents against bacterial diseases has been revived during the last years. Bacteriophage therapy constitutes a promising alternative not only for treatment, but also for prevention of vibriosis in aquaculture. However, several scientific and technological challenges still need further investigation before reliable, reproducible treatments with commercial potential are available for the aquaculture industry. The potential and the challenges of phage-based alternatives to antibiotic treatment of vibriosis are addressed in this review.
Climatology and Interannual Variability of Winter North Pacific Storm Track in CMIP5 Models
Authors: Yang Minghao , Li Xin , Zuo Ruiting , Chen Xiong , Wang Liqiong
Published: February 2018

We examine the capability of thirteen Coupled Model Intercomparison Project (CMIP) phase 5 (CMIP5) models in simulating climatology and interannual variability of Winter North Pacific Storm Track (WNPST). It is found that nearly half of the selected models can reproduce the spatial pattern of WNPST climatology. However, the strength and spatial variation of WNPST climatology are weak in most of the models. Most differences among the models are in the northeast of the simulated multi-model ensemble (MME) climatology, while it is more consistent in the south. The MME can reflect not only the center position, but also the strength and spatial distribution of interannual variation of the WNPST amplitude. Except for CNRM-CM5, the interannual standard deviations of simulated WNPST strength and spatial variation in all other models are weak. ACCESS1-3 and CanESM2 have a better capability in simulating the spatial modes of WNPST, while the simulated second and third modes in some models are in opposite order with those in NCEP (National Centers for Environmental Prediction) reanalysis. Only five models and MME can capture “midwinter suppression” feature in their simulations. Compared with NCEP reanalysis, the winter longitude index is larger and latitude index is smaller in most of the models, indicating the simulated storm track is further east and south. CNRM-CM5, MME and CMCC-CM could be used to evaluate interannual variation of strength index, longitude index and latitude index respectively. Nevertheless, only INM-CM4 and CNRM-CM5 can simulate southward drift of WNPST.
Impacts of Traffic Tidal Flow on Pollutant Dispersion in a Non-Uniform Urban Street Canyon
Authors: Ming Tingzhen , Fang Weijie , Peng Chong , Cai Cunjin , De Richter Renaud , Ahmadi Mohammad , Wen Yuangao
Published: February 2018

A three-dimensional geometrical model was established based on a section of street canyons in the 2nd Ring Road of Wuhan, China, and a mathematical model describing the fluid flow and pollutant dispersion characteristics in the street canyon was developed. The effect of traffic tidal flow was investigated based on the measurement results of the passing vehicles as the pollution source of the CFD method and on the spatial distribution of pollutants under various ambient crosswinds. Numerical investigation results indicated that: (i) in this three-dimensional asymmetrical shallow street canyon, if the pollution source followed a non-uniform distribution due to the traffic tidal flow and the wind flow was perpendicular to the street, a leeward side source intensity stronger than the windward side intensity would cause an expansion of the pollution space even if the total source in the street is equal. When the ambient wind speed is 3 m/s, the pollutant source intensity near the leeward side that is stronger than that near the windward side (R = 2, R = 3, and R = 5) leads to an increased average concentration of CO at pedestrian breathing height by 26%, 37%, and 41%, respectively. (R is the ratio parameter of the left side pollution source and the right side pollution source); (ii) However, this feature will become less significant with increasing wind speeds and changes of wind direction; (iii) the pollution source intensity exerted a decisive influence on the pollutant level in the street canyon. With the decrease of the pollution source intensity, the pollutant concentration decreased proportionally.
Assessment of Bacterial Aerosol in a Preschool, Primary School and High School in Poland
Authors: Brągoszewska Ewa , Mainka Anna , Pastuszka Józef , Lizończyk Katarzyna , Desta Yitages
Published: February 2018

The issue of healthy educational buildings is a global concern because children are particularly at risk of lung damage and infection caused by poor indoor air quality (IAQ). This article presents the results of a preliminary study of the concentration and size distribution of bacterial aerosol in three educational buildings: a preschool, primary school, and high school. Sampling was undertaken in the classrooms with an Andersen six-stage impactor (with aerodynamic cut-off diameters of 7.0, 4.7, 3.3, 2.1, 1.1 and 0.65 μm) during spring 2016 and 2017, as well as the outside of the buildings. After incubation, bioaerosol particles captured on nutrient media on Petri dishes were quantitatively evaluated and qualitatively identified. The highest average concentration of bacterial aerosol was inside the primary school building (2205 CFU/m3), whereas the lowest average concentration of indoor culturable bacteria was observed in the high school building (391 CFU/m3). Using the obtained data, the exposure dose (ED) of the bacterial aerosol was estimated for children attending each educational level. The most frequently occurring species in the sampled bacterial aerosol were Gram-positive cocci in the indoor environment and Gram-positive rod-forming endospores in the outdoor environment.
Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests
Authors: Mauk Michael , Song Jinzhao , Liu Changchun , Bau Haim
Published: February 2018

Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, with emphasis on minimally instrumented, point-of-care (POC) tests for resource-limited settings. Microfluidic cartridges (‘chips’) that combine solid-phase nucleic acid extraction; isothermal enzymatic nucleic acid amplification; pre-stored, paraffin-encapsulated lyophilized reagents; and real-time or endpoint optical detection are described. These chips can be used with a companion module for separating plasma from blood through a combined sedimentation-filtration effect. Three reporter types: Fluorescence, colorimetric dyes, and bioluminescence; and a new paradigm for end-point detection based on a diffusion-reaction column are compared. Multiplexing (parallel amplification and detection of multiple targets) is demonstrated. Low-cost detection and added functionality (data analysis, control, communication) can be realized using a cellphone platform with the chip. Some related and similar-purposed approaches by others are surveyed.
Post-Translational Modifications of H2A Histone Variants and Their Role in Cancer
Authors: Corujo David , Buschbeck Marcus
Published: February 2018

Histone variants are chromatin components that replace replication-coupled histones in a fraction of nucleosomes and confer particular characteristics to chromatin. H2A variants represent the most numerous and diverse group among histone protein families. In the nucleosomal structure, H2A-H2B dimers can be removed and exchanged more easily than the stable H3-H4 core. The unstructured N-terminal histone tails of all histones, but also the C-terminal tails of H2A histones protrude out of the compact structure of the nucleosome core. These accessible tails are the preferential target sites for a large number of post-translational modifications (PTMs). While some PTMs are shared between replication-coupled H2A and H2A variants, many modifications are limited to a specific histone variant. The present review focuses on the H2A variants H2A.Z, H2A.X, and macroH2A, and summarizes their functions in chromatin and how these are linked to cancer development and progression. H2A.Z primarily acts as an oncogene and macroH2A and H2A.X as tumour suppressors. We further focus on the regulation by PTMs, which helps to understand a degree of context dependency.
Clinically Usable Interleukin 12 Plasmid without an Antibiotic Resistance Gene: Functionality and Toxicity Study in Murine Melanoma Model
Authors: Kamensek Urska , Tesic Natasa , Sersa Gregor , Cemazar Maja
Published: February 2018

Plasmids, which are currently used in interleukin 12 (IL-12) gene electrotransfer (GET) clinical trials in the USA, contain antibiotic resistance genes and are thus, according to the safety recommendation of the European Medicines Agency (EMA), not suitable for clinical trials in the EU. In the current study, our aim was to prepare an IL-12 plasmid without an antibiotic resistance gene and test its functionality and toxicity after GET in a preclinical B16F10 mouse melanoma model. The antibiotic resistance-free plasmid encoding the human IL-12 fusion gene linked to the p21 promoter, i.e., p21-hIL-12-ORT, was constructed using operator-repressor titration (ORT) technology. Next, the expression profile of the plasmid after GET was determined in B16F10 cells and tumors. Additionally, blood chemistry, hematological and histological changes, and antitumor response were evaluated after GET of the plasmid in melanoma tumors. The results demonstrated a good expression and safety profile of the p21-hIL-12-ORT GET and indications of efficacy. We hope that the obtained results will help to accelerate the transfer of this promising treatment from preclinical studies to clinical application in the EU.
Teaming Up for Trouble: Cancer Cells, Transforming Growth Factor-β1 Signaling and the Epigenetic Corruption of Stromal Naïve Fibroblasts
Authors: Lamprecht Sergio , Sigal-Batikoff Ina , Shany Shraga , Abu-Freha Naim , Ling Eduard , Delinasios George J. , Moyal-Atias Keren , Delinasios John G. , Fich Alexander
Published: February 2018

It is well recognized that cancer cells subvert the phenotype of stromal naïve fibroblasts and instruct the neighboring cells to sustain their growth agenda. The mechanisms underpinning the switch of fibroblasts to cancer-associated fibroblasts (CAFs) are the focus of intense investigation. One of the most significant hallmarks of the biological identity of CAFs is that their tumor-promoting phenotype is stably maintained during in vitro and ex vivo propagation without the continual interaction with the adjacent cancer cells. In this review, we discuss robust evidence showing that the master cytokine Transforming Growth Factor-β1 (TGFβ-1) is a prime mover in reshaping, via epigenetic switches, the phenotype of stromal fibroblasts to a durable state. We also examine, in detail, the pervasive involvement of TGFβ-1 signaling from both cancer cells and CAFs in fostering cancer development, taking colorectal cancer (CRC) as a paradigm of human neoplasia. Finally, we review the stroma-centric anticancer therapeutic approach focused on CAFs—the most abundant cell population of the tumor microenvironment (TME)—as target cells.
Improving the Tribological Properties of Spark-Anodized Titanium by Magnetron Sputtered Diamond-Like Carbon
Authors: Chen Zhaoxiang , Ren Xipeng , Ren Limei , Wang Tengchao , Qi Xiaowen , Yang Yulin
Published: February 2018

Spark-anodization of titanium can produce adherent and wear-resistant TiO2 film on the surface, but the spark-anodized titanium has lots of surface micro-pores, resulting in an unstable and high friction coefficient against many counterparts. In this study, the diamond-like carbon (DLC) was introduced into the micro-pores of spark-anodized titanium by the magnetron sputtering technique and a TiO2/DLC composite coating was fabricated. The microstructure and tribological properties of TiO2/DLC composite coating were investigated and compared with the anodic TiO2 mono-film and DLC mono-film. Results show that the DLC deposition significantly decreased the surface roughness and porosity of spark-anodized titanium. The fabricated TiO2/DLC composite coating exhibited a more stable and much lower friction coefficient than anodic TiO2 mono-film. Although the friction coefficient of the composite coating and the DLC mono-film was similar under both light load and heavy load conditions, the wear life of the composite coating was about 43% longer than that of DLC mono-film under heavy load condition. The wear rate of titanium with protective composite coating was much lower than that of titanium with DLC mono-film. The superior low friction coefficient and wear rate of the TiO2/DLC composite coating make it a good candidate as protective coating on titanium alloys.
Modulating the Partitioning of Microparticles in a Polyethylene Glycol (PEG)-Dextran (DEX) Aqueous Biphasic System by Surface Modification
Authors: Byun Chang , Kim Minkyung , Kim Daehee
Published: February 2018

Aqueous two-phase systems (ATPSs) or aqueous biphasic systems are useful for biological separation/preparation and cell micropatterning. Specifically, aqueous two-phase systems (ATPSs) are not harmful to cells or biomaterials; therefore, they have been used to partition and isolate these materials from others. In this study, we suggest chemically modifying the surface of target materials (micro/nanoparticles, for example) with polymers, such as polyethylene glycol and dextran, which are the same polymer solutes as those in the ATPS. As a simple model, we chemically coated polyethylene glycol or dextran to the surface of polystyrene magnetic particles and observed selective partitioning of the surface modified particles to the phase in which the same polymer solutes are dominant. This approach follows the principle “like dissolves like” and can be expanded to other aqueous biphasic or multiphasic systems while consuming fewer chemicals than the conventional modulation of hydrophobicities of solute polymers to control partitioning in aqueous biphasic or multiphasic systems.
Nano Diesel Soot Particles Reduce Wear and Friction Performance Using an Oil Additive on a Laser Textured Surface
Authors: Peng Jin-Fang , Shen Ming-Xue , Cai Zhen-Bing
Published: February 2018

Tribological properties of nano diesel soot (DS) as an additive were investigated. Textures in linear radiating arrays were prepared on the surface of a spring-steel plate by laser radiation. The texture densities were 19.6%, 22.1%, and 44.2%, and the depth was 30 μm. The results indicated that the textured surface was interacted with additive favorably to improve its tribological performance. Friction coefficients and wear rates of textured surfaces with additive in oil were generally much lower compared to the original surface without additive. The higher area density of the textured surface with the additive in oil had the lowest friction coefficient, as low as 0.12, and also the minimum wear rate, as low as 1 × 103 μm/N·m in 100 °C, to be achievable. Such results can be attributed to the formation of the tribo-film and the storage function of the micro-dimple.
The Role of Conformational Entropy in the Determination of Structural-Kinetic Relationships for Helix-Coil Transitions
Authors: Rudzinski Joseph , Bereau Tristan
Published: February 2018

Coarse-grained molecular simulation models can provide significant insight into the complex behavior of protein systems, but suffer from an inherently distorted description of dynamical properties. We recently demonstrated that, for a heptapeptide of alanine residues, the structural and kinetic properties of a simulation model are linked in a rather simple way, given a certain level of physics present in the model. In this work, we extend these findings to a longer peptide, for which the representation of configuration space in terms of a full enumeration of sequences of helical/coil states along the peptide backbone is impractical. We verify the structural-kinetic relationships by scanning the parameter space of a simple native-biased model and then employ a distinct transferable model to validate and generalize the conclusions. Our results further demonstrate the validity of the previous findings, while clarifying the role of conformational entropy in the determination of the structural-kinetic relationships. More specifically, while the global, long timescale kinetic properties of a particular class of models with varying energetic parameters but approximately fixed conformational entropy are determined by the overarching structural features of the ensemble, a shift in these kinetic observables occurs for models with a distinct representation of steric interactions. At the same time, the relationship between structure and more local, faster kinetic properties is not affected by varying the conformational entropy of the model.
Editorial of the Special Issue of the 10th Workshop on Biomedical and Bioinformatics Challenges for Computer Science—BBC 2017
Authors: Agapito Giuseppe , Cannataro Mario , Castelli Mauro , Dondi Riccardo , Zoppis Italo
Published: February 2018

In this special issue, we present two of the papers presented at the 10th Workshop on Biomedical and Bioinformatics Challenges for Computer Science—BBC2017, held in Zurich, 12–14 June 2017.
Synthesis and Fluorescence Properties of Asymmetrical Salamo-Type Tetranuclear Zinc(II) Complex
Authors: Peng Yun-Dong , Li Xiao-Yan , Kang Quan-Peng , An Gao-Xian , Zhang Yang , Dong Wen-Kui
Published: February 2018

A new tetranuclear zinc(II) complex with an asymmetrical Salamo-type chelating ligand, H3L (5-methoxy-6′-hydroxy-2,2′-[ethylenedioxybis(nitrilomethylidyne)]diphenol), was synthesized and characterized using FT-IR, elemental analyses, X-ray single crystal diffraction method, UV-Vis, and fluorescence spectra. The zinc(II) complex possesses the cell parameters a = 8.1960(7) Å, b = 9.8127(8) Å, c = 16.5428(15) Å, Z = 1, V = 1172.5(2) Å3, R1 = 0.0722, and wR2 = 0.1558, and crystallizes in the triclinic system, with space group P-1. X-ray crystal structure analysis reveals that Zn1 and Zn2 atoms are all pentacoordinated and adopt slightly twisted tetragonal pyramidal and trigonal bipyramidal geometries. The zinc(II) complex forms a 1D supramolecular chain via intermolecular hydrogen bonds along the b axis. Besides, the fluorescence properties have been discussed.
Effect of Thermally Reduced Graphene Oxide on Mechanical Properties of Woven Carbon Fiber/Epoxy Composite
Authors: Adak Nitai , Chhetri Suman , Murmu Naresh , Samanta Pranab , Kuila Tapas
Published: February 2018

Thermally reduced graphene oxide (TRGO) was incorporated as a reinforcing filler in the epoxy resin to investigate the effect on the mechanical properties of carbon fiber (CF)/epoxy composites. At first, the epoxy matrix was modified by adding different wt % of TRGO from 0.05 to 0.4 wt % followed by the preparation of TRGO/CF/epoxy composites througha vacuum-assisted resin transfer molding process. The prepared TRGO was characterized by using Fourier transform infrared spectroscopy, Raman Spectroscopy and field emission scanning electron microscopy (FE-SEM) techniques. It was observed that the wrinkled structure of synthesized TRGO may be helpful to interlock with the epoxy resin and CF.The inter-laminar shear strength, in-plane fracture toughness and impact strength increased by ~67%, 62% and 93% at 0.2 wt % of TRGO loading in the CF/epoxy composites as compared to the CF reinforced epoxy. The mechanical properties of the hybrid composites decreased beyond the 0.2 wt % of TRGO incorporation in the epoxy resin. The fracture surfaces of the hybrid composites were studied by FE-SEM image analysis to investigate the synergistic effect of TRGO in the CF/epoxy composite. This study suggested that TRGO could be used asgood nanofiller to resist the matrix and fiber fracture.
Twenty Years of Tomato Breeding at EPSO-UMH: Transfer Resistance from Wild Types to Local Landraces—From the First Molecular Markers to Genotyping by Sequencing (GBS)
Authors: Carbonell Pedro , Alonso Aranzazu , Grau Adrián , Salinas Juan , García-Martínez Santiago , Ruiz Juan
Published: February 2018

In 1998, the plant breeding team at the School of Engineering of Orihuela (EPSO), part of the Miguel Hernández University (UMH) in Elche, commenced a tomato breeding program. Marker-assisted selection and backcrossing were used to simultaneously introduce three genes (Tm-2a, Ty-1, and Sw-5) that confer resistance to relevant viruses, such as tomato mosaic virus (ToMV), tomato yellow curl virus (TYLCV), and tomato spotted wilt virus (TSWV), to traditional varieties of local tomatoes, specifically the “Muchamiel” and the “De la pera” types. After each backcross, cleaved amplified polymorphic sequence (CAPS) molecular markers were used to select the plants with the resistance genes of interest. A previously described marker was used for TSWV, and new markers were designed for ToMV, and TYLCV using available sequences in the National Center for Biotechnology Information (NCBI) database. In parallel to the breeding program, several molecular markers—Sequence Related Amplified Polymorphism (SRAP), Simple Sequence Repeats (SSRs), Amplified Fragment Length Polymorphisms (AFLPs), Single Nucleotide Polymorphisms (SNPs), and (GATA)4 probes—were used to study genetic variability, and to identify a collection of Spanish and Italian traditional tomato varieties. The results showed a limited genetic variability among cultivated tomato varieties. The breeding lines Muchamiel UMH 1200, and De la pera 1203 (both with homozygous resistance to the three viruses) were the first new varieties that were obtained. They were included in the Register of Protected Plant Varieties in 2013. Lines without a resistance to TYLCV were also developed, and protected in 2017. We have begun to use SNP massive genotyping for studies of genetic association, and for selecting plants with the Ty-1 gene with less linkage drag. Molecular markers have been extremely useful in identifying the different steps of the tomato breeding program at EPSO-UMH.
A Cross-Layer Biometric Recognition System for Mobile IoT Devices
Authors: Taheri Shayan , Yuan Jiann-Shiun
Published: February 2018

A biometric recognition system is one of the leading candidates for the current and the next generation of smart visual systems. The visual system is the engine of the surveillance cameras that have great importance for intelligence and security purposes. These surveillance devices can be a target of adversaries for accomplishing various malicious scenarios such as disabling the camera in critical times or the lack of recognition of a criminal. In this work, we propose a cross-layer biometric recognition system that has small computational complexity and is suitable for mobile Internet of Things (IoT) devices. Furthermore, due to the involvement of both hardware and software in realizing this system in a decussate and chaining structure, it is easier to locate and provide alternative paths for the system flow in the case of an attack. For security analysis of this system, one of the elements of this system named the advanced encryption standard (AES) is infected by four different Hardware Trojansthat target different parts of this module. The purpose of these Trojans is to sabotage the biometric data that are under process by the biometric recognition system. All of the software and the hardware modules of this system are implemented using MATLAB and Verilog HDL, respectively. According to the performance evaluation results, the system shows an acceptable performance in recognizing healthy biometric data. It is able to detect the infected data, as well. With respect to its hardware results, the system may not contribute significantly to the hardware design parameters of a surveillance camera considering all the hardware elements within the device.
Disturbance Alters the Relative Importance of Topographic and Biogeochemical Controls on Microbial Activity in Temperate Montane Forests
Authors: Lybrand Rebecca A. , Gallery Rachel E. , Trahan Nicole A. , Moore David J. P.
Published: February 2018

Fire and pathogen-induced tree mortality are the two dominant forms of disturbance in Western U.S. montane forests. We investigated the consequences of both disturbance types on the controls of microbial activity in soils from 56 plots across a topographic gradient one year after the 2012 High Park wildfire in Colorado. Topsoil biogeochemistry, soil CO2 efflux, potential exoenzyme activities, and microbial biomass were quantified in plots that experienced fire disturbance, beetle disturbance, or both fire and beetle disturbance, and in plots where there was no recent evidence of disturbance. Soil CO2 efflux, N-, and P-degrading exoenzyme activities in undisturbed plots were positively correlated with soil moisture, estimated from a topographic wetness index; coefficient of determinations ranged from 0.5 to 0.65. Conversely, the same estimates of microbial activities from fire-disturbed and beetle-disturbed soils showed little correspondence to topographically inferred wetness, but demonstrated mostly negative relationships with soil pH (fire only) and mostly positive relationships with DOC/TDN (dissolved organic carbon/total dissolved nitrogen) ratios for both disturbance types. The coefficient of determination for regressions of microbial activity with soil pH and DOC/TDN reached 0.8 and 0.63 in fire- and beetle-disturbed forests, respectively. Drivers of soil microbial activity change as a function of disturbance type, suggesting simple mathematical models are insufficient in capturing the impact of disturbance in forests.
Influence of Scarification on the Germination Capacity of Acorns Harvested from Uneven-Aged Stands of Pedunculate Oak (Quercus robur L.)
Authors: Kaliniewicz Zdzisław , Tylek Paweł
Published: February 2018

Scarification involves the partial removal of the seed coat on the side of the hilum, opposite the radicle, to speed up germination in acorns. The aim of this study was to determine the influence of scarification on the germination capacity of pedunculate oak acorns, selected and prepared for sowing. The diameter, length and mass of acorns were measured before and after scarification in four batches of acorns harvested from uneven-aged trees (76, 91, 131 and 161 years). The measured parameters were used to determine the correlations between acorn dimensions and mass, and to calculate the dimensional scarification index and the mass scarification index in acorns. Individual complete and scarified acorns from every batch were germinated on sand and peat substrate for 28 days. The analyzed acorns were characterized by average size and mass. Scarification decreased acorn mass by around 22% and acorn length by around 31% on average. Scarification and the elimination of infected acorns increased germination capacity from around 64% to around 81% on average. Acorns can be divided into size groups before scarification to obtain seed material with varied germination capacity. Larger acorns with higher germination capacity can be used for sowing in container nurseries, whereas smaller acorns with lower germination capacity can be sown in open-field nurseries.
Wnt Signaling in Stem Cell Maintenance and Differentiation in the Drosophila Germarium
Authors: Waghmare Indrayani , Page-McCaw Andrea
Published: February 2018

Wnt signaling is a conserved regulator of stem cell behaviors, and the Drosophila germarium has been an important model tissue for the study of stem cell maintenance, differentiation, and proliferation. Here we review Wnt signaling in the germarium, which houses two distinct types of ovarian stem cells: the anteriorly located germline stem cells (GSCs), which give rise to oocytes; and the mid-posteriorly located follicle stem cells (FSCs), which give rise to the somatic follicle cells that cover a developing oocyte. The maintenance and proliferation of GSCs and FSCs is regulated by the stem cell niches, whereas differentiation of the germline is regulated by the differentiation niche. Four distinct Wnt ligands are localized in the germarium, and we focus review on how these Wnt ligands and Wnt signaling affects maintenance and differentiation of both germline and follicle stem cells in their respective niches.
Examples of Application of GASAKe for Predicting the Occurrence of Rainfall-Induced Landslides in Southern Italy
Authors: Terranova Oreste , Gariano Stefano , Iaquinta Pasquale , Lupiano Valeria , Rago Valeria , Iovine Giulio
Published: February 2018

GASAKe is an empirical-hydrological model aimed at forecasting the time of occurrence of landslides. Activations can be predicted of either single landslides or sets of slope movements of the same type in a homogeneous environment. The model requires a rainfall series and a set of dates of landslide activation as input data. Calibration is performed through genetic algorithms, and allows for determining a family of optimal kernels to weight antecedent rainfall properly. As output, the mobility function highlights critical conditions of slope stability. Based on suitable calibration and validation samples of activation dates, the model represents a useful tool to be integrated in early-warning systems for geo-hydrological risk mitigation purposes. In the present paper, examples of application to three rock slides in Calabria and to cases of soil slips in Campania are discussed. Calibration and validation are discussed, based on independent datasets. Obtained results are either excellent for two of the Calabrian rock slides or just promising for the remaining case studies. The best performances of the model take advantage of an accurate knowledge of the activation history of the landslides, and a proper hydrological characterization of the sites. For such cases, GASAKe could be usefully employed within early-warning systems for geo-hydrological risk mitigation and Civil Protection purposes. Finally, a new release of the model is presently under test: its innovative features are briefly presented.
Comparison of the Performance of Six Drought Indices in Characterizing Historical Drought for the Upper Blue Nile Basin, Ethiopia
Authors: Bayissa Yared , Maskey Shreedhar , Tadesse Tsegaye , Van Andel Schalk , Moges Semu , Van Griensven Ann , Solomatine Dimitri
Published: February 2018

The Upper Blue Nile (UBN) basin is less-explored in terms of drought studies as compared to other parts of Ethiopia and lacks a basin-specific drought monitoring system. This study compares six drought indices: Standardized Precipitation Index (SPI), Standardized Precipitation Evaporation Index (SPEI), Evapotranspiration Deficit Index (ETDI), Soil Moisture Deficit Index (SMDI), Aggregate Drought Index (ADI), and Standardized Runoff-discharge Index (SRI), and evaluates their performance with respect to identifying historic drought events in the UBN basin. The indices were calculated using monthly time series of observed precipitation, average temperature, river discharge, and modeled evapotranspiration and soil moisture from 1970 to 2010. The Pearson’s correlation coefficients between the six drought indices were analyzed. SPI and SPEI at 3-month aggregate period showed high correlation with ETDI and SMDI (r > 0.62), while SPI and SPEI at 12-month aggregate period correlate better with SRI. The performance of the six drought indices in identifying historic droughts: 1973–1974, 1983–1984, 1994–1995, and 2003–2004 was analyzed using data obtained from Emergency Events Database (EM-DAT) and previous studies. When drought onset dates indicated by the six drought indices are compared with that in the EM-DAT. SPI, and SPEI showed early onsets of drought events, except 2003–2004 drought for which the onset date was unavailable in EM-DAT. Similarly, ETDI, SMDI and SRI-3 showed early onset for two drought events and late onsets in one-drought event. In contrast, ADI showed late onsets for two drought events and early onset for one drought event. None of the six drought indices could individually identify the onsets of all the selected historic drought events; however, they may identify the onsets when combined by considering several input variables at different aggregate periods.
Health Care Utilisation by Bullying Victims: A Cross-Sectional Study of A 9-Year-Old Cohort in Ireland
Authors: Hayes Catherine , Kelly Dervla , Taut Cristina , Nixon Elizabeth , Zgaga Lina , Williams James , O’Dowd Thomas , Reulbach Udo
Published: February 2018

Children frequently refrain from disclosing being bullied. Early identification of bullying by healthcare professionals in children may prevent adverse health consequences. The aim of our study was to determine whether Health Care Utilisation (HCU) is higher in 9-year-olds who report being bullied and factors influencing type of HCU. The study consists of cross-sectional surveys of Child Cohort of Irish National Longitudinal Study of Children (Wave 1), 8,568 9-year-olds, and their carers. Being bullied was assessed by a self-reported questionnaire completed by children at home. HCU outcomes consisted of the following: visits to GP, Mental Health Practitioner (MHP), Emergency Department (ED), and nights in hospital by parent interview. Bivariate logistic regression and gender-stratified Poisson models were used to determine association. Victimisation by bullying independently increased visits to GP (OR 1.13, 95% confidence interval (CI): 1.03 to 1.25; p = 0.02), MHP (OR 1.31, 95% CI: 1.05 to 1.63; p = 0.02), though not ED visits (OR 0.99, 95% CI: 0.87 to 1.13; p = 0.8) or nights in hospital (OR 1.07 95% CI: 0.97 to 1.18; p = 0.2), adjusting for underlying chronic condition(s) and socio-demographic confounders. Victimised girls made higher GP visits (RR 1.14, 95% CI: 1.06 to 1.23; p < 0.001) and spent more nights in hospital (RR 1.10, 95% CI: 1.04 to 1.15; p < 0.001). Victimised boys were more likely to contact MHPs (RR 1.21, 95% CI: 1.02 to 1.44; p = 0.03). 9-year-old bullied subjects were more likely to utilise primary care services than non-bullied 9-year-olds. Different HCU patterns were observed according to gender and gender differences in the presentation of victimisation. Our findings may lead to the development of clinical practice guidelines for early detection and appropriate management of bullied children.
A Text Structuring Method for Chinese Medical Text Based on Temporal Information
Authors: Zhang Runtong , Chu Fuzhi , Chen Donghua , Shang Xiaopu
Published: February 2018

Chinese Electronic Medical Records (EMRs) contains a large number of complex medical free text which includes a variety of information, such as temporal information, patients’ symptoms and laboratory data. However, as an important knowledge base, these unstructured text data in EMR are hard to process directly by computer to support further medical research. This paper proposes a novel text structuring method to extract knowledge from EMR texts and reorganize them in chronological order according to the temporal information in the text. By implementing some entropy-based algorithms as contrast, experiments evaluate the performance of the proposed method, which indicates the new method can significantly reduce the complexity of EMR text. This work is significant in structuring the EMR free text into temporal-structured data for further medical analysis.
Social Force Model-Based Group Behavior Simulation in Virtual Geographic Environments
Authors: Huang Lin , Gong Jianhua , Li Wenhang , Xu Tao , Shen Shen , Liang Jianming , Feng Quanlong , Zhang Dong , Sun Jun
Published: February 2018

Virtual geographic environments (VGEs) are extensively used to explore the relationship between humans and environments. Crowd simulation provides a method for VGEs to represent crowd behaviors that are observed in the real world. The social force model (SFM) can simulate interactions among individuals, but it has not sufficiently accounted for inter-group and intra-group behaviors which are important components of crowd dynamics. We present the social group force model (SGFM), based on an extended SFM, to simulate group behaviors in VGEs with focuses on the avoiding behaviors among different social groups and the coordinate behaviors among subgroups that belong to one social group. In our model, psychological repulsions between social groups make them avoid with the whole group and group members can stick together as much as possible; while social groups are separated into several subgroups, the rear subgroups try to catch up and keep the whole group cohesive. We compare the simulation results of the SGFM with the extended SFM and the phenomena in videos. Then we discuss the function of Virtual Reality (VR) in crowd simulation visualization. The results indicate that the SGFM can enhance social group behaviors in crowd dynamics.
Non-Native Conformational Isomers of the Catalytic Domain of PCSK9 Induce an Immune Response, Reduce Lipids and Increase LDL Receptor Levels
Authors: Jiang Chuantao , Nischal Hersharan , Sun Hua , Li Li , Cao Ying , Wei Peng , Chang Jui-Yoa , Teng Ba-Bie
Published: February 2018

PCSK9 (Proprotein convertase subtilisin/kexin type 9) increases plasma cholesterol levels by promoting LDL receptor degradation. Current antibody inhibitors block the interaction between PCSK9 and LDL receptors, significantly decrease plasma cholesterol levels, and provide beneficial clinical outcomes. To reduce the action of PCSK9 in plasma, a novel strategy that will produce a panel of non-native, conformationally-altered isomers of PCSK9 (X-PCSK9) to develop active immunotherapy targeting of native PCSK9 and inhibiting/blocking the interaction of PCSK9 with LDL receptor, thus decreasing plasma cholesterol levels is proposed. The authors used the scrambled disulfide bond technique to generate conformationally-altered isomers of the catalytic domain of mouse PCSK9. The focus was on the immune response of four X-isomers and their effects on plasma cholesterol and triglyceride levels in both C57BL/6J and Apoe−/− mice. The authors showed that the four immunogens produced significant immunogenicity against native PCSK9 to day 120 after immunization of C57BL/6J and Apoe−/− mice. This resulted in significantly decreased plasma cholesterol levels in C57BL/6J mice, and to a lesser degree in Apoe−/− mice. The X-PCSK9-B1 treated mice had increased LDL receptor mRNA and protein levels at day 120 after treatment. Thus, this study provides a new, potentially promising approach that uses long-term immunotherapy for a treatment of hypercholesterolemia.
Targeting Glioblastoma Cells Expressing CD44 with Liposomes Encapsulating Doxorubicin and Displaying Chlorotoxin-IgG Fc Fusion Protein
Authors: Mahmud Hafizah , Kasai Tomonari , Khayrani Apriliana Cahya , Asakura Mami , Oo Aung Ko Ko , Du Juan , Vaidyanath Arun , El-Ghlban Samah , Mizutani Akifumi , Seno Akimasa , Murakami Hiroshi , Masuda Junko , Seno Masaharu
Published: February 2018

We recently have established a successful xenograft model of human glioblastoma cells by enriching hyaluronic acid-dependent spheroid-forming populations termed U251MG-P1 cells from U251MG cells. Since U251MG-P1 cells have been confirmed to express CD44 along with principal stemness marker genes, OCT3/4, SOX2, KLF4 and Nanog, this CD44 expressing population appeared to majorly consist of undifferentiated cells. Evaluating the sensitivity to anti-cancer agents, we found U251MG-P1 cells were sensitive to doxorubicin with IC50 at 200 nM. Although doxorubicin has serious side-effects, establishment of an efficient therapy targeting undifferentiated glioblastoma cell population is necessary. We previously designed a chlorotoxin peptide fused to human IgG Fc region without hinge sequence (M-CTX-Fc), which exhibited a stronger growth inhibitory effect on the glioblastoma cell line A172 than an original chlorotoxin peptide. Combining these results together, we designed M-CTX-Fc conjugated liposomes encapsulating doxorubicin and used U251MG-P1 cells as the target model in this study. The liposome modified with M-CTX-Fc was designed with a diameter of approximately 100–150 nm and showed high encapsulation efficiency, adequate loading capacity of anticancer drug, enhanced antitumor effects demonstrating increasing uptake into the cells in vitro; M-CTX-Fc-L-Dox shows great promise in its ability to suppress tumor growth in vivo and it could serve as a template for targeted delivery of other therapeutics.
Chronic Blockade of Brain Endothelin Receptor Type-A (ETA) Reduces Blood Pressure and Prevents Catecholaminergic Overactivity in the Right Olfactory Bulb of DOCA-Salt Hypertensive Rats
Authors: Cassinotti Luis , Guil María , Schöller Mercedes , Navarro Mónica , Bianciotti Liliana , Vatta Marcelo
Published: February 2018

Overactivity of the sympathetic nervous system and central endothelins (ETs) are involved in the development of hypertension. Besides the well-known brain structures involved in the regulation of blood pressure like the hypothalamus or locus coeruleus, evidence suggests that the olfactory bulb (OB) also modulates cardiovascular function. In the present study, we evaluated the interaction between the endothelinergic and catecholaminergic systems in the OB of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Following brain ET receptor type A (ETA) blockade by BQ610 (selective antagonist), transcriptional, traductional, and post-traductional changes in tyrosine hydroxylase (TH) were assessed in the OB of normotensive and DOCA-salt hypertensive rats. Time course variations in systolic blood pressure and heart rate were also registered. Results showed that ETA blockade dose dependently reduced blood pressure in hypertensive rats, but it did not change heart rate. It also prevented the increase in TH activity and expression (mRNA and protein) in the right OB of hypertensive animals. However, ETA blockade did not affect hemodynamics or TH in normotensive animals. Present results support that brain ETA are not involved in blood pressure regulation in normal rats, but they significantly contribute to chronic blood pressure elevation in hypertensive animals. Changes in TH activity and expression were observed in the right but not in the left OB, supporting functional asymmetry, in line with previous studies regarding cardiovascular regulation. Present findings provide further evidence on the role of ETs in the regulation of catecholaminergic activity and the contribution of the right OB to DOCA-salt hypertension.
Towards the Development of a More Accurate Monitoring Procedure for Invertebrate Populations, in the Presence of an Unknown Spatial Pattern of Population Distribution in the Field
Authors: Petrovskaya Natalia B. , Forbes Emily , Petrovskii Sergei V. , Walters Keith F. A.
Published: February 2018

Studies addressing many ecological problems require accurate evaluation of the total population size. In this paper, we revisit a sampling procedure used for the evaluation of the abundance of an invertebrate population from assessment data collected on a spatial grid of sampling locations. We first discuss how insufficient information about the spatial population density obtained on a coarse sampling grid may affect the accuracy of an evaluation of total population size. Such information deficit in field data can arise because of inadequate spatial resolution of the population distribution (spatially variable population density) when coarse grids are used, which is especially true when a strongly heterogeneous spatial population density is sampled. We then argue that the average trap count (the quantity routinely used to quantify abundance), if obtained from a sampling grid that is too coarse, is a random variable because of the uncertainty in sampling spatial data. Finally, we show that a probabilistic approach similar to bootstrapping techniques can be an efficient tool to quantify the uncertainty in the evaluation procedure in the presence of a spatial pattern reflecting a patchy distribution of invertebrates within the sampling grid.
C-Reactive Protein to Albumin Ratio Predicts 30-Day and 1-Year Mortality in Postoperative Patients after Admission to the Intensive Care Unit
Authors: Oh Tak , Ji Eunjeong , Na Hyo-seok , Min Byunghun , Jeon Young-Tae , Do Sang-Hwan , Song In-Ae , Park Hee-Pyoung , Hwang Jung-Won
Published: February 2018

C-reactive protein and albumin are associated with poor outcomes in critically ill patients. This study aimed to investigate the significance of the C-reactive protein/albumin (CRP/ALB) ratio as a novel prognostic factor for 30-day or 1-year mortality after admission to the postoperative intensive care unit (ICU). This retrospective study was conducted by examining the medical records of postoperative patients aged 19 years or older admitted to the ICU at a tertiary care hospital from January 2007 to July 2016. This study included data on 11,832 postoperative patients in the analysis. The cut-off value of the CRP/ALB ratio was set at 1.75 and 1.58 for 30-day and 1-year mortality after ICU admission, respectively. In postoperative patients with a high CRP/ALB ratio (>1.75 or >1.58), the probability of death within 30 days or 1 year after ICU admission were 30% or 43% higher than that in the group with the least CRP/ALB ratio, respectively (≤1.75 or ≤1.58)(p < 0.05). This study found the CRP/ALB ratio measured immediately after ICU admission to be an independent risk factor for 30-day and 1-year mortality in postoperative patients admitted to the ICU.