bluuebunny/crossref_metadata_2025 huggingface dataset:
Unveiling the Effect of Particle Incorporation in PEO Coatings on the Corrosion and Wear Performance of Magnesium Implants
Authors: Almajidi Yasir Q. , Ali Eyhab , Jameel Madiha Fouad , Saleh Luma Hussain , Aggarwal Saurabh , Zearah Sajad Ali , Alamula Abbas Firras , Alsaalamy Ali , Sharifianjazi Fariborz , Bathaei Masoud Soroush
Published: December 2023

Magnesium has been a focal point of significant exploration in the biomedical engineering domain for many years due to its exceptional attributes, encompassing impressive specific strength, low density, excellent damping abilities, biodegradability, and the sought-after quality of biocompatibility. The primary drawback associated with magnesium-based implants is their susceptibility to corrosion and wear in physiological environments, which represents a significant limitation. Research findings have established that plasma electrolytic oxidation (PEO) induces substantial modifications in the surface characteristics and corrosion behavior of magnesium and its alloy counterparts. By subjecting the surface to high voltages, a porous ceramic coating is formed, resulting in not only altered surface properties and corrosion resistance, but also enhanced wear resistance. However, a drawback of the PEO process is that excessive pore formation and porosity within the shell could potentially undermine the coating’s corrosion and wear resistances. Altering the electrolyte conditions by introducing micro- and nano-particles can serve as a valuable approach to decrease coating porosity and enhance their ultimate characteristics. This paper evaluates the particle adhesion, composition, corrosion, and wear performances of particle-incorporated coatings applied to magnesium alloys through the PEO method.
Study on the Lubricating Characteristics of the Oil Film of the Slipper Pair in a Large Displacement Piston Pump
Authors: Xu Liping , Chen Jiaheng , Li Donglin , Zhang Liang , Jia Yaowei , Guo Fuhang , Li Jian
Published: December 2023

Due to the large size of the bottom surface, the slipper pair of the large displacement piston pump (LDPP) will form a larger linear speed difference in the inner and outer positions of the slipper relative to the center of the swash plate during high-speed rotation. It is more likely to lead to the slipper overturning, which makes the slipper partially worn. To make improvements, the comprehensive performance of the slipper pair of the LDPP, the motion law of the slipper pair of the LDPP was explored. Firstly, a mathematical model of the oil film thickness of the slipper pair of the LDPP under the state of residual compression force is established, based on the consideration of the linear velocity difference formed by the high-speed rotation of the large bottom surface slipper and the theory of dynamics and thermodynamics. Secondly, the impact of rotational speed, piston chamber pressure and oil temperature on the oil film thickness of the slipper pair was simulated and analyzed. Finally, to measure the oil film thickness of the slipper pair, oil film thickness measuring equipment was created, and the accuracy of the mathematical model was verified. The study revealed the changing rules of the oil film thickness and tilt angle of the bottom surface of the slipper pair under various working conditions. The consistency of the simulation and test findings demonstrates that the mathematical model can accurately describe influencing elements and changing rules of the LDPP slipper pair’s oil film lubrication characteristics.
Research on a Method of Robot Grinding Force Tracking and Compensation Based on Deep Genetic Algorithm
Authors: Meng Minghui , Zhou Chuande , Lv Zhongliang , Zheng Lingbo , Feng Wei , Wu Ting , Zhang Xuewei
Published: December 2023

In the grinding process of complex-shaped cast workpieces, discrepancies between the workpiece’s contours and their corresponding three-dimensional models frequently lead to deviations in the machining trajectory, resulting in instances of under-grinding or over-grinding. Addressing this challenge, this study introduces an advanced robotic grinding force automatic tracking technique, leveraging a combination of deep neural networks and genetic algorithms. Harnessing the capability of force sensing, our method dynamically recalibrates the grinding path, epitomizing truly flexible grinding. Initially, in line with the prerequisites for force and pose tracking, an impedance control strategy was developed, integrating pose deviations with force dynamics. Subsequently, to enhance steady-state force tracking, we employed a genetic algorithm to compensate for force discrepancies caused by positional errors. This was built upon the foundational concepts of the three-dimensional model, impedance control, and environmental parameter estimation, leading to an optimized grinding trajectory. Following tracking tests, it was observed that the grinding’s normal force was consistently controlled within the bracket of 20 ± 2.5 N. To further substantiate our methodology, a specialized experimental platform was established for grinding complex-shaped castings. Optimized strategies were employed under anticipated forces of 5 N, 10 N, and 15 N for the grinding tests. The results indicated that the contact forces during the grinding process remained stable at 5 ± 1 N, 10 ± 1.5 N, and 15 ± 2 N. When juxtaposed with conventional teaching grinding methods, our approach manifested a reduction in grinding forces by 71.4%, 70%, and 75%, respectively. Post-grinding, the workpieces presented a pronounced enhancement in surface texture, exhibiting a marked increase in surface uniformity. Surface roughness metrics, originally recorded at 17.5 μm, 17.1 μm, and 18.7 μm, saw significant reductions to 1.5 μm, 1.6 μm, and 1.4 μm, respectively, indicating reductions by 76%, 73%, and 78%. Such outcomes not only meet the surface finishing standards for complex-shaped castings but also offer an efficacious strategy for robot-assisted flexible grinding.
Social Ranking Problems at the Interplay between Social Choice Theory and Coalitional Games
Authors: Fritz Felix , Moretti Stefano , Staudacher Jochen
Published: December 2023

This paper reviews the recent literature on the “social ranking problem”, that is, the problem of converting group rankings into individual rankings. We introduce and categorize existing social ranking methods and we briefly explain their attributes. Three main categories of social ranking methods are identified: lexicographic social rankings, methods based on voting mechanisms, and those inspired by the theory of coalitional games. An open-source R package called socialranking for computing the majority of the existing social rankings is also presented and discussed.
Combining Surgical Innovations in Amputation Surgery—Robotic Harvest of the Rectus Abdominis Muscle, Transplantation and Targeted Muscle Reinnervation Improves Myocontrol Capability and Pain in a Transradial Amputee
Authors: Ernst Jennifer , Hahne Janne , Markovic Marko , Schilling Arndt , Lorbeer Lisa , Grade Marian , Felmerer Gunther
Published: December 2023

Adding robotic surgery to bionic reconstruction might open a new dimension. The objective was to evaluate if a robotically harvested rectus abdominis (RA) transplant is a feasible procedure to improve soft-tissue coverage at the residual limb (RL) and serve as a recipient for up to three nerves due to its unique architecture and to allow the generation of additional signals for advanced myoelectric prosthesis control. A transradial amputee with insufficient soft-tissue coverage and painful neuromas underwent the interventions and was observed for 18 months. RA muscle was harvested using robotic-assisted surgery and transplanted to the RL, followed by end-to-end neurroraphy to the recipient nerves of the three muscle segments to reanimate radial, median, and ulnar nerve function. The transplanted muscle healed with partial necrosis of the skin mesh graft. Twelve months later, reliable, and spatially well-defined Hoffmann–Tinel signs were detectable at three segments of the RA muscle flap. No donor-site morbidities were present, and EMG activity could be detected in all three muscle segments. The linear discriminant analysis (LDA) classifier could reliably distinguish three classes within 1% error tolerance using only the three electrodes on the muscle transplant and up to five classes outside the muscle transplant. The combination of these surgical procedure advances with emerging (myo-)control technologies can easily be extended to different amputation levels to reduce RL complications and augment control sites with a limited surface area, thus facilitating the usability of advanced myoelectric prostheses.
Evolution of Irradiation Defects in W and W-Re Systems: A Density Functional Theory and Rate Theory Study
Authors: Xin Tianyuan , Yang Yiying , Wang Yuexia , Wu Lu , Pan Rongjian , Xu Qiu , Wu Xiaoyong
Published: December 2023

In a fusion environment, tungsten, a plasma-facing material in a reactor, is subject to the irradiation of high-energy neutrons, generating a large amount of displacement damage and transmutation products (such as rhenium, Re). We studied the evolution of defects under irradiation in W and W-Re systems using the density functional theory (DFT) and rate theory (RT) method. The results indicate that the evolution of irradiation defects is mainly affected by the irradiation dose, dose rate, and temperature. During irradiation, loops form first in W, followed by the generation of voids, which are due to the different migration energies of point defects. Higher dose rates result in a higher density and larger size of defects in tungsten. Higher temperatures cause a decrease in void density and an increase in size. The results obtained at 600 °C were in good agreement with the reported TEM data. In W-Re alloys, it is indicated that the formation of loops is delayed because Re suppresses the nucleation of loops. The dynamic introduction of Re in W stabilizes the growth of defects compared to W-Re alloys, suggesting that transmuting elements have less detrimental effects on irradiation than alloying. As defect densities and sizes were quantified under different irradiation conditions, the results provide data for the multi-scale simulation of the radiation damage and thermal/mechanical properties in plasma-facing materials under fusion conditions.
Modification of Johnson–Cook Constitutive Parameters in Ball Burnish Simulation of 7075-T651 Aluminum Alloy
Authors: Yin Danqing , Zhao Haoqi , Chen Yunfeng , Chang Junming , Wang Yonglei , Wang Xiaoqiang
Published: December 2023

The Johnson–Cook model is widely used because of its ability to meet the simulation material requirements in various situations. However, under different working conditions, the accuracy of the model may have errors. In this work, based on Abaqus (Abaqus R2022 Education Edition, Dassault Systèmes, Paris, France) simulation software, Johnson–Cook (JC) constitutive model parameters and compression parameters were selected to simulate the improvement of surface roughness during the ball milling of a 7075-T651 aluminum alloy. In the numerical simulation of ball polishing, the changes in the residual stress field when the Johnson–Cook parameters change are analyzed. By comparing the numerically simulated residual stress fields under the JC parameters calculated at different strain rates, a set of parameters that agrees with the experimental values is selected. The results show that the residual stresses are in line with the experimental values when the strain rate is 10−4s −1. The changes in roughness corresponding to the selected parameters are analyzed. The results indicate that the simulated trend of roughness variation is consistent with the experimental values, and the optimal surface roughness value under a static pressure of 300 N is obtained.
Metabolic and Other Endocrine Elements with Regard to Lifestyle Choices: Focus on E-Cigarettes
Authors: Osman Andrei , Petrescu Gabriel Sebastian , Tuculină Mihaela Jana , Dascălu Ionela Teodora , Popescu Cristina , Enescu Anca-Ștefania , Dăguci Constantin , Cucu Anca-Pati , Nistor Claudiu , Carsote Mara
Published: December 2023

Our objective was to overview recent data on metabolic/endocrine disorders with respect to e-cigarette (e-cig) use. This is a narrative review; we researched English, full-length, original articles on PubMed (between January 2020 and August 2023) by using different keywords in the area of metabolic/endocrine issues. We only included original clinical studies (n = 22) and excluded case reports and experimental studies. 3 studies (N1 = 22,385; N2 = 600,046; N3 = 5101) addressed prediabetes risk; N1 showed a 1.57-fold increased risk of dual vs. never smokers, a higher risk that was not confirmed in N2 (based on self-reported assessments). Current non-smokers (N1) who were dual smokers still have an increased odd of prediabetes (a 1.27-fold risk increase). N3 and another 2 studies addressed type 2 diabetes mellitus (DM): a lower prevalence of DM among dual users (3.3%) vs. cigarette smoking (5.9%) was identified. 6 studies investigated obesity profile (4 of them found positive correlations with e-cig use). One study (N4 = 373,781) showed that e-cig use was associated with obesity in the general population (OR = 1.6, 95%CI: 1.3–2.1, p < 0.05); another (N5 = 7505, 0.82% were e-cig-only) showed that obesity had a higher prevalence in dual smokers (51%) vs. cig-only (41.2%, p < 0.05), while another (N6 = 3055) found that female (not male) e-cig smokers had higher body mass index vs. non-smokers. Data on metabolic syndrome (MS) are provided for dual smokers (n = 2): one case–control study found that female dual smokers had higher odds of MS than non-smokers. The need for awareness with respect to potential e-cig-–associated medical issues should be part of modern medicine, including daily anamnesis. Whether the metabolic/endocrine frame is part of the general picture is yet to be determined. Surveillance protocols should help clinicians to easily access the medical background of one subject, including this specific matter of e-cig with/without conventional cigarettes smoking and other habits/lifestyle elements, especially when taking into consideration metabolism anomalies.
The Angiopoietin Signaling Pathway Is Involved in Inflammatory Processes in Hospitalized COVID-19 Patients
Authors: Mendoza Rachelle P. , Momeni Amir , Saha Nayanendu , Arshi Juwairiya , Gabutan Elmer C. , Alejandro Nichole , Zuretti Alejandro , Premsrirut Prem K. , Nikolov Dimitar B.
Published: December 2023

The viral agent SARS-CoV-2 clearly affects several organ systems, including the cardiovascular system. Angiopoietins are involved in vascular integrity and angiogenesis. Angiopoietin-1 (Ang1) promotes vessel stabilization, while angiopoietin-2 (Ang2), which is usually expressed at low levels, is significantly elevated in inflammatory and angiogenic conditions. Interleukin-6 (IL-6) is known to induce defective angiogenesis via the activation of the Ang2 pathway. Vasculitis and vasculopathy are some of the defining features of moderate to severe COVID-19-associated systemic disease. We investigated the serum levels of angiopoietins, as well as interleukin-6 levels and anti-SARS-CoV2 IgG titers, in hospitalized COVID-19 patients across disease severity and healthy controls. Ang2 levels were elevated in COVID-19 patients across all severity compared to healthy controls, while Ang1 levels were decreased. The patients with adverse outcomes (death and/or prolonged hospitalization) had relatively lower and stable Ang1 levels but continuously elevated Ang2 levels, while those who had no adverse outcomes had increasing levels of both Ang1 and Ang2, followed by a decrease in both. These results suggest that the dynamic levels of Ang1 and Ang2 during the clinical course may predict adverse outcomes in COVID-19 patients. Ang1 seems to play an important role in controlling Ang2-related inflammatory mechanisms in COVID-19 patients. IL-6 and anti-SARS-CoV2 spike protein IgG levels were significantly elevated in patients with severe disease. Our findings represent an informative pilot assessment into the role of the angiopoietin signaling pathway in the inflammatory response in COVID-19.
Assessing Freshwater Microbiomes from Different Storage Sources in the Caribbean Using DNA Metabarcoding
Authors: Cross Joseph , Honnavar Prasanna , Quidet Xegfred , Butler Travis , Shivaprasad Aparna , Christian Linroy
Published: December 2023

Next-generation sequencing (NGS) and the technique of DNA metabarcoding have provided more efficient and comprehensive options for testing water quality compared to traditional methods. Recent studies have shown the efficacy of DNA metabarcoding in characterizing the bacterial microbiomes of varied sources of drinking water, including rivers, reservoirs, wells, tanks, and lakes. We asked whether DNA metabarcoding could be used to characterize the microbiome of different private sources of stored freshwater on the Caribbean Island nation of Antigua and Barbuda. Two replicate water samples were obtained from three different private residential sources in Antigua: a well, an above-ground tank, and a cistern. The bacterial microbiomes of different freshwater sources were assessed using 16S rRNA metabarcoding. We measured both alpha diversity (species diversity within a sample) and beta diversity (species diversity across samples) and conducted a taxonomic analysis. We also looked for the presence of potentially pathogenic species. Major differences were found in the microbiome composition and relative abundances depending on the water source. A lower alpha diversity was observed in the cistern sample compared to the others, and distinct differences in the microbiome composition and relative abundance were noted between the samples. Notably, pathogenic species, or genera known to harbor such species, were detected in all the samples. We conclude that DNA metabarcoding can provide an effective and comprehensive assessment of drinking water quality and has the potential to identify pathogenic species overlooked using traditional methods. This method also shows promise for tracing the source of disease outbreaks due to waterborne microorganisms. This is the first study from small island countries in the Caribbean where metabarcoding has been applied for assessing freshwater water quality.
A Combination of Blue Light at 460 nm and H2O2 for the Safe and Effective Eradication of Staphylococcus aureus in an Infected Mouse Skin Abrasion Model
Authors: Ngo Vu Nguyen , Truong Thien Nguyen Thuan , Tran Tin Trung , Nguyen Loan Thanh , Mach Ngoc Bao , Vu Van Van , Nguyen Thi Thu Hoai , Vu Thiet Minh
Published: December 2023

Antibiotic-free approaches are more important than ever to address the rapidly growing problem of the antibiotic resistance crisis. The photolysis of the bacterial virulence factor staphyloxanthin using blue light at 460 nm (BL460 nm) has been found to effectively attenuate Staphylococcus aureus to chemical and physical agents. However, phototherapy using BL640 nm still needs to be investigated in detail for its safety in eradicating Staphylococcus aureus in vitro and in vivo. In this study, we employed a 460 nm continuous-wavelength LED source and a low concentration of hydrogen peroxide to treat S. aureus under a culturing condition and a wound abrasion mouse model. The results demonstrated the safety of the combined therapy when it did not modify the bacterial virulence factors or the susceptibility to widely used antibiotics. In addition, the results of the mouse model also showed that the combined therapy was safe to apply to mouse skin since it did not cause adverse skin irritation. More importantly, the therapy can aid in healing S. aureus-infected wounds with an efficacy comparable to that of the topical antibiotic Fucidin. The aforementioned findings indicate that the concurrent application of BL460 nm and hydrogen peroxide can be used safely as an alternative or adjunct to antibiotics in treating S. aureus-infected wounds.
Critical Review of Tuberculosis Diagnosis in Children from Papua New Guinea Presenting to Health Facilities in the Torres Strait Islands, Australia
Authors: Foster J’Belle , Marais Ben J. , Mendez Diana , McBryde Emma S.
Published: December 2023

Paediatric tuberculosis can be challenging to diagnose, and various approaches are used in different settings. A retrospective review was conducted on Papua New Guinea (PNG) children with presumptive TB who presented for health care in the Torres Strait Islands, Australia, between 2016 and 2019. We compared diagnostic algorithms including the modified Keith Edwards TB Score, The Union Desk Guide, and the new World Health Organization (WHO) algorithm, with diagnostic practices used in the remote Torres Strait Islands. Of the 66 children with presumptive TB, 7 had bacteriologically confirmed TB. The majority (52%) were under 5 years (median age 61 months), and 45% were malnourished. There was moderate agreement across the diagnostic methods (K = 0.34; 95% CI 0.23–0.46), with the highest concordance observed between The Union Desk Guide and the WHO’s algorithm (K = 0.61). Local TB physicians might have over-diagnosed presumed lymph node TB while under-diagnosing TB overall. Enhancing the precision and promptness of paediatric TB diagnosis using practical tools is pivotal to decrease TB-related child mortality, notably in isolated regions like the Torres Strait and the Western Province of PNG.
The Flotation Separation Mechanism of Smithsonite from Calcite and Dolomite with Combined Collectors
Authors: Chen Xiangxiang , Bai Junzhi , Zhang Zhaoyang , Qiang Wen , Huang Shiyi , Ouyang Yunfei , Liu Tianhao , Yin Wanzhong
Published: December 2023

Smithsonite, dolomite, and calcite are carbonate minerals. The crystal structures and spatial distribution characteristics of their common surface metal sites are similar, leading to difficulty in the flotation separation of smithsonite from these carbonate gangues. In this paper, the floatability of smithsonite, dolomite, and calcite in sodium oleate, salicylhydroxamic acid, and their combined-collector system were systematically studied through single-mineral flotation tests, respectively. The results showed that it was difficult to obtain a noticeable recovery difference between smithsonite–calcite and smithsonite–dolomite in a single-collector system of sodium oleate and salicylhydroxamic acid, both at the same time. In the combined-collector system of salicylhydroxamic acid and sodium oleate with total dosage of 6 × 10−4 mol/L, molar ratio of 3:1, and pH of 8.0, the recovery difference of smithsonite–calcite and smithsonite–dolomite could reach the highest values of 38.46% and 37.98%, respectively, while obtaining the highest smithsonite recovery of 88.19%. The adsorption mechanism of the combined collectors was investigated via Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, a collector adsorption test, and zeta potential measurements, respectively.
Modelling the Acoustic Propagation in a Test Section of a Cavitation Tunnel: Scattering Issues of the Acoustic Source
Authors: Boucheron Romuald
Published: December 2023

The prediction of the underwater-radiated noise for a vessel is classically performed at a model scale and extrapolated by semi-empirical laws. The accuracy of such a method depends on many parameters. Among them, the acoustic propagation model used to estimate the noise measured at a model scale is important. The present study focuses on the impact of the presence of a source in the transverse plane. The scattering effect, often neglected in many studies, is here investigated. Applying different methods for computation, we perform several simulations of the acoustic pressure field to show the influence of the scattered field. We finally discuss the results and draw some conclusions about the scattering effect in our experimental configuration.
Fusing Sequence and Structural Knowledge by Heterogeneous Models to Accurately and Interpretively Predict Drug–Target Affinity
Authors: Zeng Xin , Zhong Kai-Yang , Jiang Bei , Li Yi
Published: December 2023

Drug–target affinity (DTA) prediction is crucial for understanding molecular interactions and aiding drug discovery and development. While various computational methods have been proposed for DTA prediction, their predictive accuracy remains limited, failing to delve into the structural nuances of interactions. With increasingly accurate and accessible structure prediction of targets, we developed a novel deep learning model, named S2DTA, to accurately predict DTA by fusing sequence features of drug SMILES, targets, and pockets and their corresponding graph structural features using heterogeneous models based on graph and semantic networks. Experimental findings underscored that complex feature representations imparted negligible enhancements to the model’s performance. However, the integration of heterogeneous models demonstrably bolstered predictive accuracy. In comparison to three state-of-the-art methodologies, such as DeepDTA, GraphDTA, and DeepDTAF, S2DTA’s performance became more evident. It exhibited a 25.2% reduction in mean absolute error (MAE) and a 20.1% decrease in root mean square error (RMSE). Additionally, S2DTA showed some improvements in other crucial metrics, including Pearson Correlation Coefficient (PCC), Spearman, Concordance Index (CI), and R2, with these metrics experiencing increases of 19.6%, 17.5%, 8.1%, and 49.4%, respectively. Finally, we conducted an interpretability analysis on the effectiveness of S2DTA by bidirectional self-attention mechanism. The analysis results supported that S2DTA was an effective and accurate tool for predicting DTA.
Virtual Screening and ADMET Prediction to Uncover the Potency of Flavonoids from Genus Erythrina as Antibacterial Agent through Inhibition of Bacterial ATPase DNA Gyrase B
Authors: Akili Abd. Wahid Rizaldi , Hardianto Ari , Latip Jalifah , Permana Afri , Herlina Tati
Published: December 2023

The emergence of antimicrobial resistance due to the widespread and inappropriate use of antibiotics has now become the global health challenge. Flavonoids have long been reported to be a potent antimicrobial agent against a wide range of pathogenic microorganisms in vitro. Therefore, new antibiotics development based on flavonoid structures could be a potential strategy to fight against antibiotic-resistant infections. This research aims to screen the potency of flavonoids of the genus Erythrina as an inhibitor of bacterial ATPase DNA gyrase B. From the 378 flavonoids being screened, 49 flavonoids show potential as an inhibitor of ATPase DNA gyrase B due to their lower binding affinity compared to the inhibitor and ATP. Further screening for their toxicity, we identified 6 flavonoids from these 49 flavonoids, which are predicted to have low toxicity. Among these flavonoids, erystagallin B (334) is predicted to have the best pharmacokinetic properties, and therefore, could be further developed as new antibacterial agent.
The Effects of a Cinchona Supplementation on Satiety, Weight Loss and Body Composition in a Population of Overweight/Obese Adults: A Controlled Randomized Study
Authors: Chiurazzi Martina , De Conno Barbara , Di Lauro Mariastella , Guida Bruna , Nasti Gilda , Schiano Elisabetta , Stornaiuolo Mariano , Tenore Gian Carlo , Colantuoni Antonio , Novellino Ettore
Published: December 2023

Obesity is a risk factor for several diseases present worldwide. Currently, dietary changes and physical activity are considered the most effective treatment to reduce obesity and its associated comorbidities. To promote weight loss, hypocaloric diets can be supported by nutraceuticals. The aim of this study was to evaluate the effects of a hypocaloric diet associated with Cinchona succirubra supplementation on satiety, body weight and body composition in obese subjects. Fifty-nine overweight/obese adults, were recruited, randomized into two groups and treated for 2 months. The first group (32 adults) was treated with a hypocaloric diet plus cinchona supplementation (the T-group); the second one (27 adults) was treated with a hypocaloric diet plus a placebo supplementation (the P-group). Anthropometric-measurements as well as bioimpedance analysis, a Zung test and biochemical parameters were evaluated at baseline and after 60 days. T-group adults showed significant improvement in nutritional status and body composition compared to those at the baseline and in the P-group. Moreover, T-group adults did not show a reduction in Cholecystokinin serum levels compared to those of P-group adults. In conclusion, our data demonstrate that a hypocaloric diet associated with cinchona supplementation is effective in inducing more significant weight loss and the re-establishment of metabolic parameters than those obtained with a hypocaloric diet.
The Acceptance of Cream Soups with the Addition of Edible Insects (Mealworm, T. molitor; House Cricket, A. domesticus; Buffalo Worm, A. diaperinus; Grasshopper, R. differens) among Young People and Seniors in Poland
Authors: Skotnicka Magdalena , Mazurek Aleksandra , Kowalski Stanisław
Published: December 2023

Research on the acceptance of consuming insects in one’s diet shows the increasing importance of this issue in the context of a sustainable food chain and ecology. Insects represent a promising food source due to their high nutritional value, efficiency in production, and minimal environmental impact, as well as the growing awareness of ecological issues. Despite these benefits, cultural and psychological barriers hinder the acceptance of consuming insects in Western countries. In this study, an assessment was made of the acceptance level of cream-type soups made from tomatoes and white vegetables with the addition of 20% flour from four insect species: mealworm (T. molitor); house cricket (A. domesticus); buffalo worm (A. diaperinus); and grasshopper (R. differens), compared to a control sample. One hundred and four subjects (55 seniors and 49 young adults) participated in this study. The acceptance level of various soups with insect flour was evaluated, considering different sensory parameters such as appearance, smell, taste, and texture. The research showed that older people have a lower acceptance for dishes containing insects compared to young adults, but the differences in the responses given were not statistically significant (p = 0.05), rejecting the assumption that insect-based products should be mainly targeted at young people. Of all the proposed test samples, the mealworm (T. molitor) was the most acceptable insect species in the tests in both taste versions for both age groups. The average score was 6.63 points on a 10-point scale. The products with the addition of grasshopper (R. differens) were rated the lowest. The acceptance level ranged between 4.23 and 4.38 points. A multiple regression analysis showed that taste and texture had the strongest influence on the overall acceptance of these dishes, and the results obtained were highly correlated with the general opinion of the testers. The increasing acceptance level and growing interest in this type of food can be a positive step towards sustainable and efficient food production.
Comparison of Ultrastructure, Extracellular Matrix, and Drug Susceptibility in M. avium subs. hominissuis Biofilms
Authors: McManus William R. , Schorey Jeffrey S.
Published: December 2023

Pulmonary infections with Mycobacterium avium occur in susceptible individuals following exposure to the bacterium in the environment, where it often persists in biofilms. Many methods have been used to generate biofilms of M. avium, and it is unknown whether different approaches generate similar structures and cell phenotypes. To make a parallel comparison of in vitro biofilm ultrastructure, extracellular matrix (ECM) composition, and the drug susceptibility of biofilm resident bacteria, we used two published methods to generate M. avium biofilms: four-week incubation in M63 medium or 24 h exposure to dithiothreitol (DTT). Scanning electron microscopy revealed differences in the biofilm ultrastructure between the two methods, including variation in the appearance of ECM materials and morphology of resident cells, while light microscopy and staining with calcofluor white indicated that both biofilms contained polysaccharides characteristic of cellulose. Measuring the susceptibility of biofilms to degradation by enzymes suggested differences in structurally important ECM molecules, with DTT biofilms having important protein and, to a lesser extent, cellulose components, and M63 biofilms having moderate protein, cellulose, and DNA components. Both biofilms conferred resistance to the bactericidal effects of amikacin and clarithromycin, with resident cells being killed at greater than 10-fold lower rates than planktonic cells at almost all concentrations. These comparisons indicate differences in biofilm responses by M. avium under differing conditions, but also suggest common features of biofilm formation, including cellulose production and antimicrobial resistance.
Current Insights on Lipid-Based Nanosystems 2023
Authors: Silva Ana Catarina , Moreira João Nuno , Sousa Lobo José Manuel
Published: December 2023

Among the different types of nanosystems that have been investigated for therapeutic use, lipid-based ones are the most explored, as they have advantages over non-lipid nanosystems, especially for improving the transport and efficacy of drugs through different routes of administration, such as ocular, cutaneous, intranasal, and intravenous [...]
N-Substituted 2-(Benzenosulfonyl)-1-Carbotioamide Derivatives Exert Antimicrobial and Cytotoxic Effects via Aldehyde Dehydrogenase Pathway: Synthesis, In Silico and In Vitro Studies
Authors: Walczak-Nowicka Lucja , Biernasiuk Anna , Ziemichód Wojciech , Karczmarzyk Zbigniew , Kwaśnik Mateusz , Kozyra Paweł , Wysocki Waldemar , Stenzel-Bembenek Agnieszka , Kowalczuk Dorota , Herbet Mariola , Pitucha Monika
Published: December 2023

A series of N-Substituted 2-(benzenosulfonyl)-1-carbotioamide derivatives (WZ1–WZ4) were synthesized and characterized using spectral methods. A comprehensive activity study was performed for each compound. All compounds were tested for antibacterial activity. Moreover, in silico studies were carried out to determine the anticancer potential of the designed WZ1–WZ4 ligands. Based on molecular docking, aldehyde dehydrogenase was selected as a molecular target. The obtained data were compared with experimental data in vitro tests. Novel hybrids of the thiosemicarbazide scaffold and sulfonyl groups may have promising anticancer activity via the aldehyde dehydrogenase pathway. The best candidate for further studies appears to be WZ2, due to its superior selectivity in comparison to the other tested compounds.
Bioanalysis of the Ex Vivo Labile PACE4 Inhibitory Peptide Ac-[d-Leu]LLLRVK-Amba in Whole Blood Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Quantification
Authors: Sauter Max , Haag Jonas , Bay Cindy , Leuschner Florian , Haefeli Walter E. , Kuhn Tim Christian , Burhenne Jürgen
Published: December 2023

The calcium-dependent serine endoprotease PACE4 is evaluated as a therapeutic target for prostate cancer. The peptide Ac-[d-Leu]LLLRVK-amba inhibits PACE4 with high affinity and has shown efficacy in preclinical mice xenograft models of prostate cancer. To support in vivo examinations of the potential therapeutic peptide Ac-[d-Leu]LLLRVK-amba, we established a highly sensitive assay for its quantification in mouse whole blood microsamples based on UPLC-MS/MS determination. Ac-[d-Leu]LLLRVK-amba was very labile during sample processing, which was particularly pronounced in plasma. High resolution mass spectrometric investigations of the metabolism/degradation in plasma revealed that no peptide bond hydrolysis generated products were formed, leaving the cause of the observed consumption of the peptide elusive. As a consequence, whole-blood quantification was developed relying on the immediate snap-freezing of blood samples after collection and immediate sample processing after serial thawing to ensure accurate and reliable quantification. The assay was validated according to the applicable recommendations of the FDA and EMA in a range of 10–10,000 ng/mL and applied to determine the pharmacokinetics of Ac-[d-Leu]LLLRVK-amba after intravenous and intraperitoneal administration to mice. Individual pharmacokinetic profiles were assessed using four microsamplings per animal. Intraperitoneal absorption was found to be efficient, demonstrating that this well-manageable route of administration is feasible for preclinical efficacy experiments with Ac-[d-Leu]LLLRVK-amba.
Cascaded Stimulated Polariton Scattering in a Single-Pass KTP Crystal under Picosecond Pumping
Authors: Vereshchagin Konstantin A. , Vereshchagin Alexey K. , Morozov Vyacheslav B. , Tunkin Vladimir G.
Published: December 2023

Cascaded stimulated polariton scattering (SPS) under powerful picosecond pumping of 532 nm wavelength was investigated in a single pass of a KTP (KTiOPO4) crystal. Under ordinary polarization of the pump wave (relatively XOZ-plane) parametric scattering in the KTP crystal was observed. By rotation of the pump wave polarization (from ordinary to extraordinary), this scattering gradually transforms into polariton scattering. Polariton signal components (spots) with gaps between them were registered at external angles of ~2° between the neighboring spots. Five polariton signal components were detected above the pump beam, with a frequency difference between neighboring cascaded components of ~188 cm−1 (5.64 THz). The wavelength of each next component increased regularly with the angle between this component and the pump wave, so this regular sequence of polariton signal components can be regarded as a cascaded SPS.
Genetic and Environmental Factors Affecting Seed Germination
Authors: Šerá Božena , Hnilička František
Published: December 2023

Plants have evolved various strategies allowing them to be successful in heterogeneous habitats, including the number and size of the seeds they produce, mechanisms for their dispersal, seed dormancy, seed vigor, seed germination, etc [...]
The Influences of Soil and Meteorological Factors on the Growth and Fruit Quality of Chinese Jujube (Ziziphus jujuba Mill.)
Authors: Duan Yanjun , Liu Shuang , Zhu Ying , Wang Yongkang , Yan Fenfen , Liu Zhiguo , Shi Xiaoxin , Liu Ping , Liu Mengjun
Published: December 2023

Chinese jujube (Ziziphus jujuba Mill.) is attracting more and more attention worldwide due to their tasty and nutritious fruit with extremely high contents of vitamin C (Vc) and soluble sugar. In order to find out the main factors that influence jujube growth and reproductive adaptability, the phenological periods, vegetative growths, fruiting abilities, and fruit qualities of eight newly released cultivars were compared and comprehensively analyzed in three representative ecological sites of the three main jujube-producing regions including Fuping (Hebei), Taigu (Shanxi), and Alar (Xinjiang) in China. Our results showed that the characteristics of jujube cultivars were significantly affected by soil and meteorological factors. The fruit number per bearing shoot was much more affected by temperature, light, and rainfall. The fruit number per bearing shoot, contents of soluble solids, and soluble sugar and Vc contents in fruits were influenced more by meteorological factors. The content of flavonoids was affected by both soil and meteorological factors. A principal component analysis (PCA) indicated that cultivars suitable for planting in Fuping (Hebei) were Yuhong and Lengbaiyu. Zaocuimi, Fucuimi, and Zaoqiuhong were suitable to be cultivated in Taigu (Shanxi), while Zaocuimi, Yuhong, Yulu, Luzao 2, and Yueguang behaved better in Alar (Xinjiang). This study provides insights of the environmental factors on jujube yield and quality and therefore provides references for highly efficient jujube cultivation.
The Beneficial Effects of Inoculation with Selected Nodule-Associated PGPR on White Lupin Are Comparable to Those of Inoculation with Symbiotic Rhizobia
Authors: Msaddak Abdelhakim , Quiñones Miguel A. , Mars Mohamed , Pueyo José J.
Published: December 2023

Nodule endophytes and associated bacteria are non-symbiotic bacteria that colonize legume nodules. They accompany nodulating rhizobia and can form beneficial associations, as some of them are plant growth-promoting rhizobacteria (PGPR) that are able to promote germination and plant growth and increase tolerance to biotic and abiotic stress. White lupin (Lupinus albus) is a legume crop that is gaining relevance as a suitable alternative to soybean as a plant protein source. Eleven nodule-associated bacteria were isolated from white lupin nodules grown in a Tunisian soil. They belonged to the genera Rhizobium, Ensifer, Pseudomonas and Bacillus. Their plant growth-promoting (PGP) and enzymatic activities were tested in vitro. Strains Pseudomonas sp., L1 and L12, displayed most PGP activities tested, and were selected for in planta assays. Inoculation with strains L1 or L12 increased seed germination and had the same positive effects on all plant growth parameters as did inoculation with symbiotic Bradyrhizobium canariense, with no significant differences among treatments. Inoculation with efficient nitrogen-fixing rhizobia must compete with rhizobia present in the soil that sometimes nodulate efficiently but fix nitrogen poorly, leading to a low response to inoculation. In such cases, inoculation with highly effective PGPR might represent a feasible alternative to boost crop productivity.
The Study of Fluid Dynamics Simulation of the Internal Flow Field in a Novel Airlift Oscillation Loop Bioreactor
Authors: Liu Anjun , Sun Xiaoyuan , Liu Dehua
Published: December 2023

The three-dimensional flow and mass transfer conditions in 5 L and 40 L airlift oscillation loop reactors were studied and compared with existing two-dimensional simulation and experimental data to verify the accuracy of the method. Then, the fluid dynamics behavior of the 2500 L reactor was simulated via supercomputing and provided guidance for production data. The results indicate that the application of oscillation operation in the 40 L multi-guide tube reactor can effectively improve the gas holdup and mass transfer coefficient in the reactor, with a maximum increase of 38% and 29%. For the 2500 L multi guide tube reactor, oscillation operation oscillation operation can significantly improve gas holdup and mass transfer coefficient increase gas holdup by 46% under 0.5 vvm operating conditions; the mass transfer coefficient increased by 54%. Therefore, oscillation operation can greatly improve the mass transfer coefficient for actual production reactors. After digging a hole in the middle sleeve, the circulating liquid speed has no effect. Although the gas holdup and mass transfer coefficient decreased by 1.3%, the gas holdup inside the entire reactor was more uniform, effectively reducing the average bubble aggregation.
Dominant Characteristics of Subject Categories in a Multiple-Category Hierarchical Scheme: A Case Study of Scopus
Authors: Kim Eungi , Jeong Da-Yeong
Published: December 2023

The Scopus journal classification method, known as All Science Journal Classification (ASJC), follows a hierarchical organization of subject categories: minor, major, and supergroups. At the minor level, journals are assigned to one or more subject categories. We refer to this classification scheme as a multiple-category hierarchical scheme. The objective of this study is to investigate the dominant characteristics of subject categories within the Scopus database and quantify their dominance using various subject indices. To conduct the study, we formulated a set of subject category indices, including the Number of Journals (J), Total Instances of Subject Categories (SC), Number of Unique Subject Categories (USC), and Dominance Index (DOMI). The results showed that high DOMI values in subject categories indicate specialization and limited associations with other fields. There were minimal correlations between DOMI and other subject category indices like J, SC, and USC, demonstrating their uniqueness and independence. The study also revealed that subject categories within the Health Sciences exhibited higher DOMI values and greater specialization compared to those in the Physical Sciences, indicating a pronounced dominance in Health Sciences minor categories. Finally, minor subject categories exhibited more variation in subject category indices compared to their upper-level subject categories, highlighting the intricate variations within the hierarchical system of the Scopus classification. These findings have implications for researchers, emphasizing the need to consider a subject category’s dominance and associations when selecting journals for their research.
The Bishopric of Maranhão and the Indian Directory: Diocesan Government and the Assimilation of Indigenous Peoples in Amazonia (1677–1798)
Authors: Muniz Pollyanna Mendonça
Published: December 2023

The second half of the eighteenth century is crucial to understanding the significant role of the Catholic Church in the many transformations experienced by indigenous peoples due to the policies of the Sebastião José de Carvalho e Melo (Marquis of Pombal) administration in Portuguese America. No study has yet to examine in depth the collaboration of the episcopate and its agents in this indigenist policy. Here, therefore, I analyse the case of the diocese of Maranhão, in the Portuguese Amazon, from its creation in 1677 until the end of the eighteenth century, demonstrating the jurisdictional dispute between the bishops and the regular clergy over the guardianship of indigenous peoples. I also examine how the appointment of clergy in former indigenous villages took place and how the diocesan structure was expanded to ensure the consolidation of Pombal’s policy amid disputes between diverse actors and interests, including those of the Portuguese state, the bishops and the indigenous population. By analysing a variety of documents using research methodologies that involve varying the scale of observation and pursuing a connected history perspective, I show how the episcopate behaved, despite its limitations and vacancies, in the process of assimilating indigenous peoples.
Emotional Experience in Human–Robot Collaboration: Suitability of Virtual Reality Scenarios to Study Interactions beyond Safety Restrictions
Authors: Legler Franziska , Trezl Jonas , Langer Dorothea , Bernhagen Max , Dettmann Andre , Bullinger Angelika C.
Published: December 2023

Today’s research on fenceless human–robot collaboration (HRC) is challenged by a relatively slow development of safety features. Simultaneously, design recommendations for HRC are requested by the industry. To simulate HRC scenarios in advance, virtual reality (VR) technology can be utilized and ensure safety. VR also allows researchers to study the effects of safety-restricted features like close distance during movements and events of robotic malfunctions. In this paper, we present a VR experiment with 40 participants collaborating with a heavy-load robot and compare the results to a similar real-world experiment to study transferability and validity. The participant’s proximity to the robot, interaction level, and occurring system failures were varied. State anxiety, trust, and intention to use were used as dependent variables, and valence and arousal values were assessed over time. Overall, state anxiety was low and trust and intention to use were high. Only simulated failures significantly increased state anxiety, reduced trust, and resulted in reduced valence and increased arousal. In comparison with the real-world experiment, non-significant differences in all dependent variables and similar progression of valence and arousal were found during scenarios without system failures. Therefore, the suitability of applying VR in HRC research to study safety-restricted features can be supported; however, further research should examine transferability for high-intensity emotional experiences.