bluuebunny/crossref_metadata_2025 huggingface dataset:
DESIGN AND SYNTHESIS OF SOME ARYLHYDRAZONE DERIVATIVES AS POTENTIAL FAAH INHIBITORS
Authors: GÜR MAZ Zehra Tuğçe , TURANLI Sümeyye , CALISKAN H. Burak
Published: January 2023

Objective: The aim was to design, synthesis and investigation of possible interactions in the enzyme active site of a series of arylhydrazone derivatives for the inhibition of the FAAH enzyme.
Material and Method: Arylhydrazone derivatives were obtained through the reaction of nicotinic hydrazide or benzohydrazide with appropriate aldehyde derivatives, and the obtained crude product was recrystallized from ethanol. After elucidating chemical structures of the compounds via spectroscopic methods, the inhibitory activities against hFAAH were screened. The results were further supported with molecular modeling studies.
Result and Discussion: In this study, a new series of seven arylhydrazone derivatives were screened against hFAAH. 4-phenoxyphenyl bearing derivative 5 was found to inhibit hFAAH 40 % at 10 µM which indicates that newly developed inhibitor could serve as a starting point for improving inhibitory effect of the new series.
PENGARUH GAYA HIDUP DAN KUALITAS PRODUK TERHADAP KEPUTUSAN PEMBELIAN (Studi Empiris Pada Konsumen di Kopi Dari Hati Madiun)
Authors:   Program Studi Manajemen (Kampus Kota Madiun), Universitas Katolik Widya Mandala Surabaya Indonesia, Suryaningtiyas Nafila , Kurniawati Dyah ,   Program Studi Manajemen (Kampus Kota Madiun), Universitas Katolik Widya Mandala Surabaya Indonesia
Published: January 2023

Penelitian ini bertujuan untuk menguji signifikansi pengaruh positif gaya hidup dan kualitas produk terhadap keputusan pembelian di Kopi Dari Hati Madiun. Sampel yang digunakan dalam penelitian ini sebanyak 100 responden yang dipilih dengan teknik non probabilty sampling dan purposive sampling. Analisis data yang digunakan dalam penelitian ini analisis regresi linear berganda dan pengujian hipotesis. Hasil penelitian menunjukkan bahwa variabel gaya hidup dan kualitas produk berpengaruh signifikan positif terhadap keputusan pembelian di Kopi Dari Hati Madiun. Nilai koefisien determinasi (R2) diperolah sejumlah 0,506 (50,6%) artinya bahwa variabel keputusan pembelian dapat dijelaskan oleh variabel gaya hidup dan kualitas produk sebesar 50,6%. Variabel yang dominan adalah variabel kualitas produk. Hal ini berarti bahwa semakin baik kualitas produk akan berdampak pada meningkatnya keputusan pembelian pada Kopi Dari Hati Madiun.
The Impact Of Diabetes Mellitus Type 2 On The Steroidogenesis Of Male Zucker Diabetic Fatty Rats
Authors: BENKO F , CHOMOVÁ M , ULIČNÁ O , ĎURAČKA M , KOVÁČ J , TVRDÁ E
Published: January 2023

The aim of this study was to evaluate the impact of diabetes mellitus type 2 (DM2) on the male endocrine system of Zucker Diabetic Fatty (ZDF) rats. Sexually mature ZDF rats were divided to a lean (control) and obese group, and had diabetes confirmed by blood tests. For the in vivo experiment, fasting blood was collected to obtain blood plasma. In case of the in vitro experiments, testicular fragments were cultured for 24 h, and the culture medium was collected. The concentrations of testosterone (T), androstenedione (A4), dehydroepiandrosterone (DHEA-S), estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were quantified in the blood plasma and the medium by the ELISA method, while cholesterol (CHOL) was assessed spectrophotometrically. A significant decline of T (36.31 %), A4 (25.11 %) and FSH (26.99 %) as well as a significant increase of CHOL and E2 (36.17 %) was observed in the blood plasma of obese ZDF rats in comparison to the control. Under in vitro conditions, a significant decrease of FSH (23.35 %) accompanied by an increase of E2 was observed in the obese group compared to the control. In the case of CHOL, LH, T, DHEA and A4 no significant differences were observed. Our results suggest that except for FSH and E2 all steroid biomolecules were synthetized normally by the testicular tissue, however a dramatic endocrine disturbance was observed at the system level. We may conclude that DM2 has negative effects on systemic hormone secretion and these alterations are more pronounced in combination with obesity.
Vérnyomáscsökkentés nagyon idős és esendő állapotú betegeknél: az alapellátásbeli gyakorlat kérdései
Authors: Torzsa Péter , Kalabay László , Csatlós Dalma , Hargittay Csenge , Márkus Bernadett , Mohos András , Szigeti Mátyás , Ferenci Tamás , Verschoor Marjolein , Rozsnyai Zsófia , Gussekloo Jacobijn , Poortvliet Rosalinde K. E. , Streit Sven
Published: January 2023

A vérnyomáscsökkentő terápiában a családorvosi gyakorlatban megmutatkozó eltérések elemzése érdekében 2543 családorvos bevonásával online kérdőíves felmérést végeztünk különböző karakterisztikájú – esendőség, SBP és CVD tekintetében –, nagyon idős (80 évesnél idősebb) betegek körében. Arra is kerestük a választ, hogy mennyire befolyásolja a kezelési döntésüket az időskori esendőség. A tanulmányunk másik célkitűzése a magyar eredmények összehasonlítása volt a nemzetközi eredményekkel. Országonként összehasonlításra került azon esetek aránya, amelyekben a kezelés mellett döntöttek a családorvosok. A 29 országból 24 országban (83%) a beteg esendő állapotát a családorvosok azzal a döntéssel kapcsolták össze, hogy inkább nem indítanak kezelést. A legalacsonyabb kezelési arány Hollandiában volt, a legmagasabb arány pedig Ukrajnában. Magyarország azon országok közé tartozott, ahol a családorvosok inkább kezelik az esendő pácienseket (a kezelési arány 50–59% között volt). A páciens esendősége nem befolyásolta a terápia megkezdését, sokkal jobban a cardiovascularis megbetegedés és a 160 Hgmm feletti SBP. A vizsgálat fontos üzenete volt, hogy továbbra is szükséges a családorvosok és a családorvos-rezidensek oktatása az idős, esendő hypertoniás betegek kezelésével kapcsolatban.
Különböző cardiovascularis rizikóbecslő pontrendszereken, a pulzushullám-terjedési sebességen és a coronaria-kalcium pontszámon alapuló artériás életkor számítási módszereinek összehasonlítása
Authors: Gyöngyösi Helga , Vecsey-Nagy Milán , Nemcsik János
Published: January 2023

Bevezetés: Az artériás (vascularis) életkor meghatározása segíthet a betegeknek a prevenciós stratégiák fontosságának megértésében. Habár számítására több módszer is létezik, ezek összehasonlításáról jelenleg nem áll rendelkezésre adat. Célkitűzés: Kutatásaink célja, hogy meghatározzuk az artériás életkort a Framingham Risk Score (FRS), a Systematic COronary Risk Evaluation Score (SCORE), a carotis-femoralis pulzushullám-terjedési sebesség (PWV), illetve a coronaria-kalcium pontszám (CACS) mérése alapján. Módszerek: Első vizsgálatunkba szív- és érrendszeri szűrőprogramon részt vevő egyének kerültek bevonásra, míg második kutatásunk betegcsoportját kis és közepes rizikójú, mellkasi panaszos betegek képezték. A PWV-t tonometriás módszerrel határoztuk meg, míg az artériás életkor meghatározása az FRS, SCORE, valamint a CACS rizikóbecslő pontszámok segítségével történt. Az artériás, illetve kronológiai életkor különbsége alapján azonosítottuk az emelkedett artériás korú egyéneket (PWV+, FRS+, SCORE+, CACS+). Eredmények: Az első vizsgálatba bevont 172 páciens esetében a PWV+ betegek közül 58 (84%) volt FRS+ is, és ez az arány a SCORE+ betegek esetében is magas volt (47/55, 85,4%). A PWV+ és a SCORE+ alanyok között azonban csak mérsékelt átfedés volt, a SCORE+ betegek közül mindössze 17 (30,9%) volt PWV+ is. A második vizsgálat 241 páciense esetén az FRS- és SCORE-alapú biológiai életkor erős korrelációt mutatott (r=0,84, p<0,001), míg a CACS alapján számított életkor mérsékelten korrelált az FRS és a SCORE alapján kalkulálttal (r=0,50 és r=0,52, mindkettő p<0,001). Következtetés: A különböző módszerrel meghatározott artériás életkorok között tapasztalt eltérések az egyes artériáséletkor-számítási módszerek további részletes összehasonlítását kívánják meg prospektív körülmények között.
Primer hyperoxaluria : Tények és perspektívák
Authors: Kaucsár Tamás , Mikes Bálint , Kelen Kata , Jávorszky Eszter , Szatmári Ildikó , Dobi Deján , Nyikuly Kinga , Várkonyi Ildikó , Maka Erika , Dezsőfi-Gottl Antal , Máttyus István , Lódi Csaba , Tory Kálmán , Sallay Péter , Szabó J. Attila , Reusz György
Published: January 2023

A primer hyperoxaluria ritka anyagcsere-betegség. A glioxilát anyagcserezavara miatt nagy mennyiségű oxalát keletkezik, amely – mivel rosszul oldódik – a szövetekben oxalátkicsapódáshoz, oxalosishoz vezet. Az oxalosis elsőként a veséket érinti, enyhébb formában urolithiasishoz, súlyos esetekben nephrocalcinosishoz és progresszív vesefunkció-romláshoz, végül végstádiumú veseelégtelenséghez vezet. A betegség típusát genetikai vizsgálattal lehet pontosan azonosítani. Klinikai megjelenése nagyon változatos. Enyhébb esetekben az oxaláttermelés csökkentése és kicsapódásának gátlása jelenthet terápiás lehetőséget, súlyosabb esetben vesepótló kezelésre lehet szükség. A betegség kóroki terápiáját egyelőre a májátültetés jelenti. Az oxalát kialakulásában kulcsszerepet játszó enzim RNS-interferencia útján történő gátlásán alapuló biológiai terápia új perspektívát jelenthet a betegség komplex kezelésében.
A krónikus vesebetegség kezelésének áttekintése, különös tekintettel a dapagliflozin alkalmazására
Authors: Barna István
Published: January 2023

A krónikus vesebetegség (CKD) a hypertonia és a diabetes gyakoriságának növekedése révén hazánkban több mint 1,5 millió embert érint. A vérnyomás céltartományban tartása csökkenti a végstádiumú vesebetegség kockázatát proteinuriás krónikus vesebetegségben szenvedő betegeknél, de a nem proteinuriás CKD-ben szenvedő betegeknél is. Az alapbetegség kezelésétől függetlenül ACE-gátlóval vagy ARB-vel történik a terápia. Az SGLT-2-gátló kezelés bizonyítottan diabeteses és nem diabeteses proteinuriás és nem proteinuriás krónikus vesebetegekben az artéria afferens vasoconstrictiójával védi a glomerulust a tubuloglomerularis feed-back révén, és a tubulussejtekben csökkentik a glükóz- és a nátriumkoncentrációt. Dapagliflozin CKD-ban alkalmazva, cukorbetegségben és nem cukorbetegek kezelésekor, a betegség progresszióját lassítja (A evidencia). Javítják a glomerularis funkciót, csökkentik a fehérjeürítést. A kezelések során az újonnan kialakuló diabeteses betegek számának csökkenése és a hematokritérték növekedése is igazolt.
Manejo ortodóntico interceptivo de los problemas oclusales resultantes de segundos molares primarios anquilosados. Reporte de caso
Authors: Valverde Montalva Hernán Renzo , Perea Paz Miguel Benjamín , ParralesValencia Christopher Carlos Eduardo
Published: January 2023

La anquilosis es una anomalía dental de etiología desconocida que provoca puentes óseos entre el cemento radicular y el hueso alveolar que puede conducirnos a diferentes problemas dentarios. El objetivo de este reporte de caso es presentar el tratamiento ortodóntico interceptivo para una maloclusión clase I en un niño masculino de 7años de edad. Este tratamiento fue propuesto considerando que el paciente presentaba dentición mixta primera fase incompleta y la ausencia clínica de piezas 55, 85, 16 y 46. Se realizó la extracción de dos piezas deciduas anquilosadas y la operculectomía de la cara oclusal de las piezas permanentes. Se utilizó el arco extraoral en el maxilar y la placa labioactiva o lip Bumper en la mandíbula lo que permitió recuperar el espacio perdido para la correcta erupción de las piezas permanentes. La intervención temprana mediante un tratamiento interceptivo permite la corrección parcial de la maloclusión e impide que se agrave el problema.
Guía clínica de elección y uso de mantenedores de espacio fijos en niños menores de 10 años. Revisión de la Literatura
Authors: Gómez Cobos Katherine Michelle , Armas Vega Ana del Carmen , Flores Jara María Gabriela
Published: January 2023

Introducción: La pérdida prematura de uno o más dientes temporales en niños menores de 10 años dada principalmente por trauma dental o acción cariogénica ocasiona la reducción del espacio de las piezas definitivas, donde se vuelve fundamental el empleo de mantenedores de espacio acorde a las necesidades del caso, ya que la elección de un método adecuado evitaría problemas posteriores tanto por la pérdida del espacio como por un uso inadecuado de estos dispositivos. Objetivo: Analizar el empleo de mantenedores fijos en ausencia de molares temporales de pacientes menores a 10 años. Materiales y métodos: Se realizó una revisión descriptiva de la literatura con empleo de estrategias de búsqueda en la base de datos PubMed publicados entre 2017 al 2022. Resultados: Con las estrategias utilizadas se obtuvieron 53 artículos, de los cuales se eliminaron los que no contenían comparaciones entre mantenedores de espacio y fallas en la cementación quedando 14 artículos que fueron revisado y expuestos a seguir. Se hace necesario la generación de estudios comparativos y a largo plazo para una estandarización de los protocolos de tratamiento, mientras tanto la elección del método para mantener el espacio y el control periódico son fundamentales para el éxito de este. Conclusión: Podemos concluir que los mantenedores de espacio fijos son los ideales en pacientes menores a 10 años con pérdida de molares temporales ya que inevitablemente se pierde el espacio de la pieza faltante y en la mayoría de casos conlleva a problemas posteriores.
Pelatihan Penulisan Naskah Ilmiah Jurnal Investigasi Volume 3 Nomor 2 September-Februari 2023 Bagi Guru-Guru se Kota Tangerang Selatan
Authors: Kusjono Gatot , Suprianto Suprianto , Syamruddin Syamruddin
Published: January 2023

Community service activities Training for Scientific Manuscript Writing of Investigative Journals Volume 3 Number 2 September-February 2023 for Teachers throughout South Tangerang City aims to assist elementary / junior high school / junior high school / high school / vocational school teachers in South Tangerang City in writing scientific manuscripts that will be published in ISSN journals. The training method is carried out using the method of assistance, discussion, workshops and technical guidance (bimtek) for writing scientific manuscripts in accordance with journal manuscript standards. The results of the training were obtained as many as 8 elementary school teacher manuscripts (57%), 4 junior high school teacher manuscripts (29%), 1 high school / vocational teacher manuscript (7%), and the P& K 1 manuscript (7%) published in the journal INVESTIGATION Volume 3 Number 2 September 2 September 2023. The publication of teacher scientific manuscripts in the journal INVESTIGATION is certainly very helpful for teachers in fulfilling the obligation to journal their scientific manuscripts, so that they are not constrained in class or position promotion.
Evaluation of Excessive Daytime Sleepiness, Anxiety and Depression Symptomatologies in Obesity
Authors: Sener Zeynep Ebru , Marakoglu Kamile , Sener Sinan
Published: January 2023

Aim: In this study, it was aimed to evaluate the excessive daytime sleepiness, anxiety and depression symptomatology in normal-weight, overweight and obese individuals.

Methods: In the study, 605 people who applied to the family medicine outpatient clinic between 2020 November 25 and 2021 March 5 were included. The questionnaire form evaluating the sociodemographic characteristics of the participants, the Hospital Anxiety and Depression Scale and the Epworth Sleepiness Scale were administered using a face-to-face interview technique.

Results: Overweight status in primary school, high school/adolescence, and university/twenties were higher in obese than in overweight. Additionally, it was higher in overweight than in normal weight. The risk of being overweight was 2.19 times higher and the risk of being obese was 6.07 times higher than those with obesity in their family compared to those who did not. Excessive daytime sleepiness was 2.95 times higher in obese than in normal weight. Anxiety symptoms were 1.97 times higher in obese than in normal weight. Depression symptoms were 2.77 times more in overweight and 2.99 times more in obese compared to normal-weight individuals.

Conclusion: As a result of this study, it was revealed that excessive daytime sleepiness, anxiety and depression symptomatologies are more common in obese compared to normal weight.

Keywords: obesity, overweight, depression, anxiety, disorders of excessive somnolence
Gelatin-grafted tubular asymmetric scaffolds promote ureteral regeneration via activation of the integrin/Erk signaling pathway
Authors: Song Baiyang , Fang Li , Mao Xufeng , Ye Xianwang , Yan Zejun , Ma Qi , Shi Zewen , Hu Yiwei , Zhu Yabin , Cheng Yue
Published: January 2023

Introduction: The repair of a diseased ureter is an urgent clinical issue that needs to be solved. A tissue-engineered scaffold for ureteral replacement is currently insufficient due to its incompetent bioactivity, especially in long-segment abnormalities. The primary reason is the failure of urothelialization on scaffolds.

Methods: In this work, we investigated the ability of gelatin-grafted tubular scaffold in ureteral repairment and its related biological mechanism. We designed various porous asymmetric poly (L-lactic acid) (PLLA)/poly (L-lactide-co-e-caprolactone) (PLCL) tubes with a thermally induced phase separation (TIPS) method via a change in the ratio of solvents (named PP). To regulate the phenotype of urothelial cells and ureteral reconstruction, gelatin was grafted onto the tubular scaffold using ammonolysis and glutaraldehyde crosslinking (named PP-gel). The in vitro and in vivo experiments were performed to test the biological function and the mechanism of the scaffolds.

Results and Discussion: The hydrophilicity of the scaffold significantly increased after gelatin grafting, which promoted the adhesion and proliferation of urothelial cells. Through subcutaneous implantation in rats, PP-gel scaffolds demonstrated good biocompatibility. The in vivo replacement showed that PP-gel could improve urothelium regeneration and maintain renal function after the ureter was replaced with an ∼4 cm-long PP-gel tube using New Zealand rabbits as the experimental animals. The related biologic mechanism of ureteral reconstruction was detected in detail. The gelatin-grafted scaffold upgraded the integrin α6/β4 on the urothelial cell membrane, which phosphorylates the focal adhesion kinase (FAK) and enhances urothelialization via the MAPK/Erk signaling pathway.

Conclusion: All these results confirmed that the PP46-gel scaffold is a promising candidate for the constitution of an engineered ureter and to repair long-segment ureteral defects.
Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis
Authors: Zhang Ning , Jiang Yue , Sun Yun-Juan , Jiang Jian-Chun , Tong Ya-Juan
Published: January 2023

Introduction: Hemicellulose is an important component in lignocellulose materials, which is second only to cellulose, accounting for 15%–35% of the dry weight of plants. In the current situation of energy shortage, making full use of lignocellulose materials to produce fuel ethanol has become an important way to solve the energy problem. Xylanase plays a crucial role in the utilization of hemicellulose. It is a necessary means to reduce the cost of hemicellulose utilization by improving the activity of xylanase. Moreover, most naturally xylanases are mesophilic enzymes, which limits their industrial application.

Methods:In this study, Myceliophthora thermophila was used to produce xylanases and a thermostable mutant M 2103 was obtained by atmospheric room temperature plasma (ARTP) mutagenesis. The research work started with exploring the effects of ARTP mutagenesis on the antioxidase system [superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO), and antioxidant capacity (AOC)] of M. thermophile, and found that superoxide dismutase activity increased by 221.13%, and polyphenol oxidase activity increased by 486.04% as compared with the original strain when the implantation time was 300 s. So as to determine the conditions for subsequent mutagenesis.

Results and Discussion:For the mutant M 2103, the reaction temperature for xylanase production remained stable in the range of 70°C–85°C. Its optimum temperature was 75°C, which was 15°C higher than that of the original strain. And its xylanase activity increased by 21.71% as compared with the original strain. M 2103 displayed a significantly higher relative xylanase activity than the original strain in the acidic (pH 4.0–7.0) range, and the xylanase activity was relatively stable in the pH range of 6.0–8.5. These results provide an alternative biocatalyst for the production of xylooligosaccharide, and a potential usage of ARTP in the mutagenesis of thermostable mutant.
Lost in transduction: Critical considerations when using viral vectors
Authors: Hines William C. , Hines William C.
Published: January 2023

The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions—and describe how differential cell-type susceptibilities can confound results. We consider the math underlying this problem and review an alternative approach to the commonly used “multiplicity of infection” (MOI) method of titering and using viral vectors in the biomedical research laboratory.
Rapid detection of influenza A viruses using a real-time reverse transcription recombinase-aided amplification assay
Authors: Cui Huan , Zhang Cheng , Tu Fei , Zhao Kui , Kong Yunyi , Pu Jie , Zhang Lei , Chen Zhaoliang , Sun Yuanyuan , Wei Yujie , Liang Chuncai , Liu Juxiang , Liu Jun , Guo Zhendong
Published: January 2023

Introduction

Influenza A viruses (IAVs) are important pathogens of respiratory infections, causing not only seasonal influenza but also influenza pandemics and posing a global threat to public health. IAVs infection spreads rapidly, widely, and across species, causing huge losses, especially zoonotic IAVs infections that are more harmful. Fast and sensitive detection of IAVs is critical for controlling the spread of this disease.

Methods

Here, a real-time reverse transcription recombinase-aided amplification (real-time RT-RAA) assay targeting conserved positions in the matrix protein gene (M gene) of IAVs, is successfully established to detect IAVs. The assay can be completed within 20 min at 42°C.

Results

The sensitivity of the real-time RT-RAA assay was 142 copies per reaction at 95% probability, which was comparable to the sensitivity of the RT-qPCR assay. The specificity assay showed that the real-time RT-RAA assay was specific to IAVs, and there was no cross-reactivity with other important viruses. In addition, 100%concordance between the real-time RT-RAA and RT-qPCR assays was achieved after testing 120 clinical specimens.

Discussion

The results suggested that the real-time RT-RAA assay we developed was a specific, sensitive and reliable diagnostic tool for the rapid detection of IAVs.
COVID-19 surveillance in wastewater: An epidemiological tool for the monitoring of SARS-CoV-2
Authors: Maryam Sajida , Ul Haq Ihtisham , Yahya Galal , Ul Haq Mehboob , Algammal Abdelazeem M. , Saber Sameh , Cavalu Simona
Published: January 2023

The coronavirus disease 2019 (COVID-19) pandemic has prompted a lot of questions globally regarding the range of information about the virus’s possible routes of transmission, diagnostics, and therapeutic tools. Worldwide studies have pointed out the importance of monitoring and early surveillance techniques based on the identification of viral RNA in wastewater. These studies indicated the presence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in human feces, which is shed via excreta including mucus, feces, saliva, and sputum. Subsequently, they get dumped into wastewater, and their presence in wastewater provides a possibility of using it as a tool to help prevent and eradicate the virus. Its monitoring is still done in many regions worldwide and serves as an early “warning signal”; however, a lot of limitations of wastewater surveillance have also been identified.
Impact of different guidewires on the implantation depth using the largest self-expandable TAVI device
Authors: Veulemans Verena , Wilde Nihal , Wienemann Hendrik , Adrichem Rik , Hokken Thijmen W. , Al-Kassou Baravan , Shamekhi Jasmin , Mauri Victor , Maier Oliver , Jung Christian , Horn Patrick , Adam Matti , Nickenig Georg , Baldus Stephan , Van Mieghem Nicolas M. , Kelm Malte , Sedaghat Alexander , Zeus Tobias
Published: January 2023

Background

The deployment process of the largest self-expandable device (STHV-34) during transcatheter aortic valve implantation (TAVI) might be challenging due to stabilization issues. Whether the use of different TAVI-guidewires impact the procedural success and outcome is not well-known. Therefore, we sought to evaluate the impact of non-Lunderquist (NLu) vs. the Lunderquist (Lu) guidewires during TAVI using the STHV-34 on the procedural and 30-day outcomes.

Methods

The primary study endpoint was defined as the final implantation depth (ID) depending on the selected guidewire strategy. Key secondary endpoints included VARC-3-defined complications.

Results

The study cohort included 398 patients of four tertiary care institutions, of whom 79.6% (317/398) had undergone TAVI using NLu and 20.4% (81/398) using Lu guidewires. Baseline characteristics did not substantially differ between NLu and Lu patients. The average ID was higher in the Lu cohort (NLu vs. Lu: −5.2 [−7.0–(−3.5)] vs. −4.5 [−6.0–(−3.0)]; p = 0.022*). The optimal ID was reached in 45.0% of patients according to former and only in 20.1% according to nowadays best practice recommendations. There was no impact of the guidewire use on the 30-day outcomes, including conduction disturbances and pacemaker need (NLu vs. Lu: 15.1 vs. 18.5%; p = 0.706).

Conclusion

The use of the LunderquistTM guidewire was associated with a higher ID during TAVI with the STHV-34 without measurable benefits in the 30-day course concerning conduction disturbances and associated pacemaker need. Whether using different guidewires might impact the outcome in challenging anatomies should be further investigated in randomized studies under standardized conditions.
CMR detects decreased myocardial deformation in asymptomatic patients at risk for heart failure
Authors: Hashemi Djawid , Doeblin Patrick , Blum Moritz , Weiss Karl Jakob , Schneider Matthias , Korosoglou Grigorios , Beyer Rebecca Elisabeth , Pieske Burkert , Edelmann Frank , Kelle Sebastian
Published: January 2023

Aims

The main management strategy of heart failure with preserved ejection fraction (HFpEF) is prevention since HFpEF is associated with many cardiovascular (CV) risk factors, especially since HFpEF is linked to a high risk for both mortality and recurrent heart failure (HF) hospitalizations. Therefore, there is a need for new tools to identify patients with a high risk profile early. Regional strain assessment by CMR seems to be superior in describing deformation impairment in HF. The MyoHealth score is a promising tool to identify cardiac changes early.

Methods and results

Heart failure patients irrespective of LVEF and asymptomatic controls were recruited, and CMR based measures were obtained. For this analysis the asymptomatic control group (n = 19) was divided into asymptomatic subjects without CV co-morbidities or evidence of cardiac abnormalities and (n = 12) and asymptomatic subjects with CV co-morbidities or evidence of cardiac abnormalities (n = 7) as well as patients with HFpEF (n = 19). We performed CMR scans at rest and during a stress test using isometric handgrip exercise (HG). Assessing the MyoHealth score at rest revealed preserved regional strain in 85 ± 9% of LV segments in controls, 73 ± 11% in at Risk subjects and 73 ± 8% in HFpEF patients. During stress the MyoHealth score was 84 ± 7% in controls, 83 ± 7 in at risk subjects and 74 ± 11 in HFpEF patients.

Conclusion

In summary, we show for the first time that asymptomatic subjects with increased CV risk present with HFpEF like impaired myocardial deformation at rest, while they show results like controls under HG stress. The potential of preventive treatment in this group of patients merits further investigation in future.

Clinical trial registration

[https://drks.de/search/de/trial/DRKS00015615], identifier [DRKS00015615].
An Olive Oil Mill Wastewater Extract Improves Chemotherapeutic Activity Against Breast Cancer Cells While Protecting From Cardiotoxicity
Authors: Benedetto Nadia , Calabrone Luana , Gutmańska Karolina , Macrì Nicoletta , Cerrito Maria Grazia , Ricotta Riccardo , Pelosi Giuseppe , Bruno Antonino , Noonan Douglas M. , Albini Adriana
Published: January 2023

Graphical Abstract

The cartoon summarized the major approaches and insights of the manuscript: (A) the possibility to recover waste material from extra-virgin olive oil (EVOO) processing that allows the repurposing of polyphenol-rich extracts characterized by (B) antiangiogenic activities in vivo, antiproliferative activities in vitro on BC cell lines (C), cardioprotective activities on rat and human cardiomyocytes (HCMs) (D), and in vivo on the zebrafish embryo heart (E).
Influence radius of pressure relief of energy-accumulating blasting and water injection in the heading face of the liangbei coal mine
Authors: Wang Zhenfeng , Wang Yu , Wang Feng
Published: January 2023

This study aims to reasonably determine the influence radius of pressure relief of energy-accumulating blasting and water injection in the Liangbei Coal Mine and effectively eliminate local stress concentration and coal seam outburst risk. The change in gas parameters in each test hole before and after energy-accumulating blasting and water injection is determined through ANSYS/LSDYNA numerical simulation, theoretical analysis and calculation, and the application of the gas parameter method in the 32051 heading face of the Liangbei Coal Mine. The influence radius of pressure relief technology of energy-accumulating blasting and water injection in the Liangbei Coal Mine in the direction of energy-accumulating trough is studied, which provides reliable data for the layout parameters of energy-accumulating blasting and water injection hole in the working face of the Liangbei Coal Mine. The experimental results are as follows: 1) The simulation via ANSYS/LSDYNA shows that a blasting cavity is formed by an explosive shock wave in the borehole. The diameter of the blasting cavity in the energy-accumulating direction is 42 cm, which is 10 times the diameter of the energy-accumulating blasting and water injection hole. The diameter of the blasting cavity in the non-energy-accumulating direction is 14 cm, which is 3.3 times the diameter of the energy-accumulating blasting and water injection hole. The stress in the energy-accumulating direction is 2.9 times larger than that in the non-energy-accumulating direction. The crack length in the energy-accumulating direction is 294 cm, and the crack length in the non-energy-accumulating direction is 80 cm. 2) Theoretical analysis and calculation present that the crushing area of shaped charge blasting is 39 cm, the fracture area is 267 cm, and the vibration area is 159 cm. 3) Comparison of the change in gas parameters of each test hole in the field experiment before and after energy-accumulating blasting and water injection indicates that the influence radius of pressure relief in the direction of energy-accumulating trough is 300 cm. 4) The layout of energy-accumulating blasting and water injection in the working face of the Liangbei Coal Mine with 550 cm borehole spacing demonstrates that the verification index of gas drilling decreases, the analytical values (Δh2) decrease from 120–140 Pa to 100–120 Pa, the drilling cutting weight (S) decreases from 3.2 to 4.6 kg/m to 3.0–3.8 kg/m, and stress is released and transferred to deep coal. This study has guiding significance for outburst elimination by local arrangement of energy-gathering blasting and water injection borehole in the working face of the Liangbei Coal Mine.
Hydrocarbon accumulation conditions and favorable exploration zones in the Lunpola Basin, Tibet
Authors: Zhang Yunbo , Wang Rui , Xu Jie , Wu Xinhe , Li Yinglie , Lin Yanhua , Wang Buqing
Published: January 2023

By systematically reviewing and summarizing the previous studies on the petroleum geology of the Lunpola Basin, combined with the latest oil and gas exploration results, the oil and gas accumulation conditions of this basin are summarized, and the favorable zones with the most exploration potential are pointed out. The results show that: ① The Paleogene mainly develops three sets of source rocks, including Niu-1 section, Niu-2 section and lower Niu-3 subsection. The lithology is mainly semi-deep and deep lacustrine gray-dark shale. Among them, the source rock of Niu-2 section is the best. However, the source rock of Niu-1 section is only developed in the Jiangjiacuo depression. The effective source rocks have the largest thickness in the central and western parts of the basin and have the best hydrocarbon-generating capacity. The thickness and abundance of the source rocks have decreased eastward. The organic matter are mainly Type I and Type II1, with a maturity of 0.58%–1.08%. ② The study area is dominated by clastic rock reservoirs, which can be divided into three types according to the strata distribution, and the overall reservoir conditions are not good. The reservoir porosity is between 0.1% and 32.6%, mainly between 2.0% and 10.0%, with an average of 6.5%; the permeability is between 0.0005 mD and 116.0 mD, mainly between 0.01 mD and 0.5 mD, which means ultra-low porosity and ultra-low permeable fracture-porous reservoirs. ③ Three sets of seals are mainly developed in the Lunpola Basin, including two regional seals of the middle Niu-2 subsection and Dingqinghu section, and a direct seal of upper Niu-2 subsection. These three sets of seals are well developed and have good preservation conditions in the relatively stable central depression and the footwall of thrust nappe structural belt . ④ The oil and gas reservoirs in the Lunpola Basin have the distribution law of “structural traps in the north and south, and lithologic traps in the center”. Structural traps are mainly distributed in the thrust nappe belt in the north and the Changshan uplift in the south, forming “self-generation and self-storage” or “lower-generation and upper-storage” reservoirs. While lithologic traps are mainly distributed in the central sag, forming “self-generation and self-storage” reservoirs. ⑤ The source-reservoir-caprock combination conditions are good in the structural traps in the footwall of the northern thrust nappe belt and the lithologic traps in the southern slope belt of Jianjiacuo depression,, and they are the most favorable oil and gas exploration zones in the Lunpola Basin.
3-D electrical structure and tectonic dynamics in the Yangbajing area based on the array magnetotelluric data
Authors: Lei Lulu , Jin Sheng , Dong Hao , Wei Wenbo , Ye Gaofeng , Zhang Letian
Published: January 2023

The well-known N-S-trending fault in the Yangbajing area plays a crucial role in the tectonic evolution of the Tibetan Plateau. Previous researches on a few E-W geophysical profiles suggested that the eastern shear at the base of the upper crust and/or lithosphere deformation brought on by asthenosphere upwelling are the major causes of the Yadong-Gulu rift’s creation. Here we propose a 3-D electrical resistivity model derived from the magnetotelluric (MT) array data spanning the Yadong-Gulu rift (YGR), and the distribution of temperature and melt fraction is estimated by the experimental calibrated relationships bridging electrical conductivity and temperature/melt fraction. The result reveals that the Indian slab subducted steeply in the east of the Yadong-Gulu rift, while Indian slab may have delaminated with a flat subduction angle in the west. The temperature distribution shows that the upper mantle of the northern Lhasa terrane is hotter than that of the southern Lhasa terrane. This is likely the result of mantle upwelling caused by either the subduction of the Indian slab or thickened Tibetan lithosphere delamination. Moreover, the strength of the mid-lower crust is so low that it may meet the conditions of the local crust flow in the west-east direction. The local crustal flow and the pulling force from the upwelling asthenosphere jointly contributed to the formation of the Yadong-Gulu rift. These main factors exist in different stages of the evolution of the Yadong-Gulu rift.
A quantitative enhanced assessment for ancient landslide reactivation risk considering cross-time scale joint response mechanism
Authors: Yang Zixi , Wu Tingchen , E Chao , Xie Xiao , Tan Leiqi , Jiang Xinxi
Published: January 2023

Ancient landslide has strong concealment and disturbance sensitivity due to its special geotechnical mechanical characteristics, and it is the potential hazard that cannot be ignored in human activities and major engineering planning. The quantitative assessment of ancient landslide reactivation risk has become more necessary for pre-disaster scientific warning. However, because the mechanisms of deformation and damage during the evolution of ancient landslides are quite complex, traditional landslide risk assessment methods only select the single-time scale and relatively stable environmental factors for analysis, lacking consideration of dynamic triggering factors such as rainfall. Focusing on the complexity, a quantitative enhanced assessment for ancient landslide reactivation risk considering cross-time scale joint response mechanism is proposed. First, on the basis of systematic analysis of the implicit genesis mechanism and explicit characterization, an evaluation system of the cross-time scale joint characteristics of ancient landslide reactivation is constructed. Then, XGBoost algorithm and SBAS-InSAR are used to establish the long-time scale developmental evolution mechanism model and the short-time scale dynamical trigger model, respectively. Subsequently, we propose a cross-time scale joint response mechanism. The information entropy weight method is applied to calculate the contribution degree of long-short time scale assessment models for ancient landslide reactivation based on the constraints of quantitative interval thresholds, and the assessment processes of different time scales are dynamically and quantitatively correlated. Finally, the updated optimization of the assessment of ancient landslide reactivation risk is achieved. In this research, experimental analysis was carried out for ancient landslide groups in a geological hazard-prone area in Fengjie County, Chongqing, a typical mountainous region of China. The results of the comparative analysis validate the superiority of the method in this paper. It helps to accurately assess the ancient landslide potential hazard in advance, providing scientific basis and technical support for the risk assessment of mountainous watershed geological hazards and major engineering projects.
Teaching vaccine development in schools: Learnings from a survey and curriculum design for a course
Authors: Ghosh Aishani , Lalsare Arav , Chirmule Narendra , Khare Ninad , Kalakuntla Pranav , Zarkar Rishi , Pawar Sachi , Sheth Smritie
Published: January 2023

Although vaccines are being developed and administered to people for more than a century, the understanding of the steps involved in vaccine development is a relatively new subject to the general public. During the current pandemic, there has been an explosion of non-validated news about COVID-19 and vaccines. To enhance the understanding of this critical societal science, there is an urgent need to teach these topics in the early education systems. Defining the essential subjects and courses for high school and developing syllabi for undergraduate courses in immunology and vaccinology can be difficult, as students choose diverse career options after their studies. To define these curricula, understanding the current level of awareness regarding vaccinology and immunology among students becomes essential. Thus, we have undertaken an exploratory survey of 650 high school and undergraduate college students in India on their awareness of the processes of vaccine development. Our results confirmed our hypothesis that there is a very limited understanding of this topic among school-going students. In this article, we propose an outline for a course for teaching in high schools. We recommend that this course should be interdisciplinary and a mix and match of majors and minors. It should train students with soft skills and prepare them for their careers in biomedical research.
Association of bone mineral density with prediabetes risk among African-American and European-American adult offspring of parents with type 2 diabetes
Authors: Liu Zhao , Asuzu Peace , Patel Avnisha , Wan Jim , Dagogo-Jack Sam
Published: January 2023

Introduction

Type 2 diabetes mellitus (T2DM) is associated with alterations in bone mineral density (BMD), but association between prediabetes and BMD is unclear.

Methods

We analyzed BMD among the initially normoglycemic participants in the Pathobiology of Prediabetes in a Biracial Cohort (POP-ABC) study in relation to incident prediabetes during 5 years of follow-up.

Results and Discussion

A total of 343 participants (193 Black, 150 White) underwent DEXA during Year 1 of POP-ABC and were followed quarterly for 5 years. The mean age was 44.2 ± 10.6 years; BMI was 30.2 ± 7.23 kg/m2. At baseline, the mean BMD was 1.176 ± 0.135 g/cm2 (1.230 ± 0.124 g/cm2 in men vs. 1.154 ± 0.134 g/cm2 in women, P<0.0001; 1.203 ± 0.114 g/cm2 in Black vs. 1.146 ± 0.150 g/cm2 in White participants, P=0.0003). During 5 years of follow-up, 101 participants developed prediabetes and 10 subjects developed T2DM (progressors); 232 were nonprogressors. Progressors to prediabetes had numerically higher baseline BMD and experienced lower 1-year decline in BMD (P<0.0001) compared with nonprogressors. From Kaplan-Meier analysis, the time to 50% prediabetes survival was 2.15 y among participants in the lowest quartile of baseline BMD, longer than those in higher quartiles (1.31 – 1.41 y). Values for BMD correlated inversely with age and adiponectin levels, and positively with BMI. In logistic regression analysis, BMD z score significantly predicted incident prediabetes: more negative BMD z scores were associated with decreased incident prediabetes (odds ratio 0.598 [95% confidence interval 0.407 - 0.877], P=0.0085), after controlling for age, BMI, change in BMI, ethnicity, blood glucose and adiponectin.

Conclusions

Among initially normoglycemic individuals, higher baseline BMD was associated with higher risk of incident prediabetes during 5 years of follow-up.
Association between transferred embryos and multiple pregnancy/live birth rate in frozen embryo transfer cycles: A retrospective study
Authors: Wu Xian , Zhou Wen-jie , Xu Bu-fang , Chen Qian , Xia Lan , Zhao Shen , Xu Hui-hui , Zhang Ai-jun , Niu Zhi-hong
Published: January 2023

Background

Physicians need an appropriate embryo transfer strategy to address the challenge of reducing multiple birth rates, while maintaining the couples’ live birth rate during assisted reproductive technology.

Methods

We included 10,060 frozen embryo transfer cycles from January 2015 to March 2020 in reproductive medical center of Ruijin hospital, Shanghai, China. Patients were grouped according to the number and grade of cleavage-stage embryo or blastocysts transferred. Live birth rate and multiple live birth rate were compared among groups of women of different ages. Multivariable logistic regression models were used to estimate the risk of multiple live birth using different combinations of transferred embryos.

Results

The transfer of double good-quality embryos was an independent predictor for multiple birth in women aged <30 years and those aged 36−39 years [<30 years: aOR =1.54 (95% CI: 1.14−2.06, P < 0.01); 36−39 years: aOR =1.84 (95% CI: 1.0−3.4, P < 0.01)]. Further, for women aged <36 years, the transfer of good-quality + poor-quality blastocysts was an independent predictor for multiple birth rate [<30 years: aOR=2.46 (95% CI: 1.45−4.18, P < 0.01); 31−35 years: aOR =4.45 (95% CI: 1.97−10.06, P < 0.01)].

Conclusions

Single-good-quality blastocyst transfer is recommended for women of all ages. When good-quality cleavage embryos are available, the choice of single or double embryo transfer with good- or average-quality embryo should depend on the age of women. Double embryo transfer with the highest possible grade of embryos is recommended for women aged ≥40 years.
Asprosin in health and disease, a new glucose sensor with central and peripheral metabolic effects
Authors: Farrag Mariam , Ait Eldjoudi Djedjiga , González-Rodríguez María , Cordero-Barreal Alfonso , Ruiz-Fernández Clara , Capuozzo Maurizio , González-Gay Miguel Angel , Mera Antonio , Lago Francisca , Soffar Ahmed , Essawy Amina , Pino Jesus , Farrag Yousof , Gualillo Oreste
Published: January 2023

Adipose tissue malfunction leads to altered adipokine secretion which might consequently contribute to an array of metabolic diseases spectrum including obesity, diabetes mellitus, and cardiovascular disorders. Asprosin is a novel diabetogenic adipokine classified as a caudamin hormone protein. This adipokine is released from white adipose tissue during fasting and elicits glucogenic and orexigenic effects. Although white adipose tissue is the dominant source for this multitask adipokine, other tissues also may produce asprosin such as salivary glands, pancreatic B-cells, and cartilage. Significantly, plasma asprosin levels link to glucose metabolism, lipid profile, insulin resistance (IR), and β-cell function. Indeed, asprosin exhibits a potent role in the metabolic process, induces hepatic glucose production, and influences appetite behavior. Clinical and preclinical research showed dysregulated levels of circulating asprosin in several metabolic diseases including obesity, type 2 diabetes mellitus (T2DM), polycystic ovarian syndrome (PCOS), non-alcoholic fatty liver (NAFLD), and several types of cancer. This review provides a comprehensive overview of the asprosin role in the etiology and pathophysiological manifestations of these conditions. Asprosin could be a promising candidate for both novel pharmacological treatment strategies and diagnostic tools, although developing a better understanding of its function and signaling pathways is still needed.
Recent advances in molecular mechanisms of acute kidney injury in patients with diabetes mellitus
Authors: Infante Barbara , Conserva Francesca , Pontrelli Paola , Leo Serena , Stasi Alessandra , Fiorentino Marco , Troise Dario , dello Strologo Andrea , Alfieri Carlo , Gesualdo Loreto , Castellano Giuseppe , Stallone Giovanni
Published: January 2023

Several insults can lead to acute kidney injury (AKI) in native kidney and transplant patients, with diabetes critically contributing as pivotal risk factor. High glucose per se can disrupt several signaling pathways within the kidney that, if not restored, can favor the instauration of mechanisms of maladaptive repair, altering kidney homeostasis and proper function. Diabetic kidneys frequently show reduced oxygenation, vascular damage and enhanced inflammatory response, features that increase the kidney vulnerability to hypoxia. Importantly, epidemiologic data shows that previous episodes of AKI increase susceptibility to diabetic kidney disease (DKD), and that patients with DKD and history of AKI have a generally worse prognosis compared to DKD patients without AKI; it is therefore crucial to monitor diabetic patients for AKI. In the present review, we will describe the causes that contribute to increased susceptibility to AKI in diabetes, with focus on the molecular mechanisms that occur during hyperglycemia and how these mechanisms expose the different types of resident renal cells to be more vulnerable to maladaptive repair during AKI (contrast- and drug-induced AKI). Finally, we will review the list of the existing candidate biomarkers of diagnosis and prognosis of AKI in patients with diabetes.