bluuebunny/crossref_metadata_2025 huggingface dataset:
Intrinsically Disordered Proteins in Chronic Diseases
Authors: Kulkarni Prakash , Uversky Vladimir
Published: April 2019

It is now increasingly evident that a large fraction of the human proteome comprises proteins that, under physiological conditions, lack fixed, ordered 3D structures as a whole or have segments that are not likely to form a defined 3D structure [...]
A Systematic Study on Design Initiation of Conceptual 3DPVS
Authors: Yuan Haobo , Xing Ke
Published: April 2019

An important product in biomedical and biomimetic engineering is the 3D scaffold, which mimics the real tissue in vitro to achieve the external cultivation of cells. The difference between the 3D scaffold and other biomimetic products lies in the fact that the former mimics the internal features of tissue, while the latter generally approximates the external traits of biological beings. In the field of scaffold engineering, the 3D printed vibratory scaffold, 3DPVS, has been proposed as a present-to-future novel scaffold product, and it currently stays at the stage of conceptual development. To achieve the novel design of the conceptual 3DPVS, a conceptual design process has been established by authors in their previous work, which contain three main stages, namely the design initiation, concept generation, and concept evaluation. In terms of design initiation, it is a ‘must-accomplish’ stage which generates outputs for both the subsequent concept generation and evaluation. Work of design initiation therefore is of significant value and it consists of several tasks; that is, conducting a thorough literature review, summarizing the fundamental issues preparing the general conceptual design, studying the multi-characterization of the 3DPVS, putting forward the potential base model(s), as well as indicating the ideality of the scaffold and establishing potential ideal model(s) for the 3DPVS. In this paper, design initiation will be chiefly focused upon these essential aspects to be discussed, work of which is expected to be useful in establishing a solid ground for future innovation work of the 3DPVS.
Breath Analysis Using eNose and Ion Mobility Technology to Diagnose Inflammatory Bowel Disease—A Pilot Study
Authors: Tiele Akira , Wicaksono Alfian , Kansara Jiten , Arasaradnam Ramesh P. , Covington James A.
Published: April 2019

Early diagnosis of inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), remains a clinical challenge with current tests being invasive and costly. The analysis of volatile organic compounds (VOCs) in exhaled breath and biomarkers in stool (faecal calprotectin (FCP)) show increasing potential as non-invasive diagnostic tools. The aim of this pilot study is to evaluate the efficacy of breath analysis and determine if FCP can be used as an additional non-invasive parameter to supplement breath results, for the diagnosis of IBD. Thirty-nine subjects were recruited (14 CD, 16 UC, 9 controls). Breath samples were analysed using an in-house built electronic nose (Wolf eNose) and commercial gas chromatograph–ion mobility spectrometer (G.A.S. BreathSpec GC-IMS). Both technologies could consistently separate IBD and controls [AUC ± 95%, sensitivity, specificity], eNose: [0.81, 0.67, 0.89]; GC-IMS: [0.93, 0.87, 0.89]. Furthermore, we could separate CD from UC, eNose: [0.88, 0.71, 0.88]; GC-IMS: [0.71, 0.86, 0.62]. Including FCP did not improve distinction between CD vs. UC; eNose: [0.74, 1.00, 0.56], but rather, improved separation of CD vs. controls and UC vs. controls; eNose: [0.77, 0.55, 1.00] and [0.72, 0.89, 0.67] without FCP, [0.81, 0.73, 0.78] and [0.90, 1.00, 0.78] with FCP, respectively. These results confirm the utility of breath analysis to distinguish between IBD-related diagnostic groups. FCP does not add significant diagnostic value to breath analysis within this study.
Establishment and Characterization of a New Intrahepatic Cholangiocarcinoma Cell Line Resistant to Gemcitabine
Authors: Varamo Chiara , Peraldo-Neia Caterina , Ostano Paola , Basiricò Marco , Raggi Chiara , Bernabei Paola , Venesio Tiziana , Berrino Enrico , Aglietta Massimo , Leone Francesco , Cavalloni Giuliana
Published: April 2019

Intrahepatic cholangiocarcinoma (ICC) is one of the most lethal liver cancers. Late diagnosis and chemotherapy resistance contribute to the scarce outfit and poor survival. Resistance mechanisms are still poorly understood. Here, we established a Gemcitabine (GEM) resistant model, the MT-CHC01R1.5 cell line, obtained by a GEM gradual exposure (up to 1.5 µM) of the sensitive counterpart, MT-CHC01. GEM resistance was irreversible, even at high doses. The in vitro and in vivo growth was slower than MT-CHC01, and no differences were highlighted in terms of migration and invasion. Drug prediction analysis suggested that Paclitaxel and Doxycycline might overcome GEM resistance. Indeed, in vitro MT-CHC01R1.5 growth was reduced by Paclitaxel and Doxycycline. Importantly, Doxycycline pretreatment at very low doses restored GEM sensitivity. To assess molecular mechanisms underlying the acquisition of GEM resistance, a detailed analysis of the transcriptome in MT-CHC01R1.5 cells versus the corresponding parental counterpart was performed. Transcriptomic analysis showed that most up-regulated genes were involved in cell cycle regulation and in the DNA related process, while most down-regulated genes were involved in the response to stimuli, xenobiotic metabolism, and angiogenesis. Furthermore, additional panels of drug resistance and epithelial to mesenchymal transition genes (n = 168) were tested by qRT-PCR and the expression of 20 genes was affected. Next, based on a comparison between qRT-PCR and microarray data, a list of up-regulated genes in MT-CHC01R1.5 was selected and further confirmed in a primary cell culture obtained from an ICC patient resistant to GEM. In conclusion, we characterized a new GEM resistance ICC model that could be exploited either to study alternative mechanisms of resistance or to explore new therapies.
Challenges and Inconsistencies in Using Lysophosphatidic Acid as a Biomarker for Ovarian Cancer
Authors: Yagi Tsukasa , Shoaib Muhammad , Kuschner Cyrus , Nishikimi Mitsuaki , Becker Lance , Lee Annette , Kim Junhwan
Published: April 2019

Increased detection of plasma lysophosphatidic acid (LPA) has been proposed as a potential diagnostic biomarker in ovarian cancer, but inconsistency exists in these reports. It has been shown that LPA can undergo an artificial increase during sample processing and analysis, which has not been accounted for in ovarian cancer research. The aim of this study is to provide a potential explanation about how the artificial increase in LPA may have interfered with previous LPA analysis in ovarian cancer research. Using an established LC-MS method, we measured LPA and other lysophospholipid levels in plasma obtained from three cohorts of patients: non-cancer controls, patients with benign ovarian tumors, and those with ovarian cancer. We did not find the LPA level to be higher in cancer samples. To understand this inconsistency, we observed that LPA content changed more significantly than other lysophospholipids as a function of plasma storage time while frozen. Additionally, only LPA was found to be adversely impacted by incubation time depending on the Ethylenediaminetetraacetic acid (EDTA) concentration used during blood drawing. We also show that the inhibition of autotaxin effectively prevented artificial LPA generation during incubation at room temperature. Our data suggests that the artificial changes in LPA content may contribute to the discrepancies reported in literature. Any future studies planning to measure plasma LPA should carefully design the study protocol to consider these confounding factors.
Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
Authors: Rodríguez Juan P. , Guijas Carlos , Astudillo Alma M. , Rubio Julio M. , Balboa María A. , Balsinde Jesús
Published: April 2019

Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated different features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of different FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a buffer system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.
Self-Templating Synthesis of 3D Hierarchical NiCo2O4@NiO Nanocage from Hydrotalcites for Toluene Oxidation
Authors: Wang Dongdong , Li Shuangde , Du Yingchao , Wu Xiaofeng , Chen Yunfa
Published: April 2019

Rational design LDHs (layered double hydroxides) with 3D hierarchical hollow structures have generated widespread interest for catalytic oxidation due to the high complexity in shell architecture and composition. Herein, we reported a handy two-step method to construct a 3D hierarchical NiCo2O4/NiO nanocage. This synthetic strategy contains a partial in situ transformation of ZIF-67 (zeolitic imidazolate framework-67) into Co-NiLDH yolk-shelled structures following ethanol etching, and a structure-preserved transformation from Co-NiLDH@ZIF-67 to a biphase nanocage following calcination. CoNi-yh-T (varied reaction time and calcination temperature) nanocages were investigated systematically by Brunauer–Emmett–Teller (BET), X-ray photoelectron spectroscopy (XPS), H2- temperature-programmed reduction (TPR), NH3-temperature-programmed desorption (TPD) and studied for toluene oxidation. The CoNi-6h-350 sample showed much higher activity with 90% toluene conversion (T90) at 229 °C at a high space velocity (SV = 60,000 mL g−1 h−1) than other catalysts (T90 >240 °C). Abundant surface high valence Co ions caused by the novel hierarchical nanostructures, together with adsorbed oxygen species and abundant medium-strength surface acid sites, played a key role for catalytic activities.
A Review of the Enhancement of Bio-Hydrogen Generation by Chemicals Addition
Authors: Sun Yong , He Jun , Yang Gang , Sun Guangzhi , Sage Valérie
Published: April 2019

Bio-hydrogen production (BHP) produced from renewable bio-resources is an attractive route for green energy production, due to its compelling advantages of relative high efficiency, cost-effectiveness, and lower ecological impact. This study reviewed different BHP pathways, and the most important enzymes involved in these pathways, to identify technological gaps and effective approaches for process intensification in industrial applications. Among the various approaches reviewed in this study, a particular focus was set on the latest methods of chemicals/metal addition for improving hydrogen generation during dark fermentation (DF) processes; the up-to-date findings of different chemicals/metal addition methods have been quantitatively evaluated and thoroughly compared in this paper. A new efficiency evaluation criterion is also proposed, allowing different BHP processes to be compared with greater simplicity and validity.
Site-Specific Addressing of Particles and Coatings via Enzyme-Mediated Destabilization
Authors: Wedegärtner David , Strube Oliver I.
Published: April 2019

Enzyme mediated addressing (EMA) is a highly specific and easy-to-apply technology for direction and deposition of particles and coatings on surfaces. Key feature of this process is an enzymatic reaction in direct proximity to the surface, which induces the deposition. The technique has previously shown great success in the handling of biological particles. In this study, addressing of non-biological nanoparticles, in particular plastics and metals, is presented. The respective particles are stabilized by an amphiphilic, enzyme-degradable block copolymer, consisting of poly(ethylene glycol) and poly(caprolactone). After contact with the enzyme pseudomonas lipase, the particles are destabilized, due to the loss of the hydrophilic part of the block copolymer. The lipase is therefore immobilized on glass supports. Immobilization is performed via adsorption or covalent bonding to epoxide groups. All deposition experiments show that addressing of individual particles occurs precisely within the predefined areas of enzyme activity. Depending on the material and reaction conditions, intact nanoparticles or coatings from such can be gained. The quintessence of the study is the indifference of the EMA regarding particle materials. From this rationale, the technique offers near unlimited materials compatibility within a precise, easy-to-apply, and upscalable process.
Histone Methylation and Memory of Environmental Stress
Authors: Fabrizio Paola , Garvis Steven , Palladino Francesca
Published: April 2019

Cellular adaptation to environmental stress relies on a wide range of tightly controlled regulatory mechanisms, including transcription. Changes in chromatin structure and organization accompany the transcriptional response to stress, and in some cases, can impart memory of stress exposure to subsequent generations through mechanisms of epigenetic inheritance. In the budding yeast Saccharomyces cerevisiae, histone post-translational modifications, and in particular histone methylation, have been shown to confer transcriptional memory of exposure to environmental stress conditions through mitotic divisions. Recent evidence from Caenorhabditis elegans also implicates histone methylation in transgenerational inheritance of stress responses, suggesting a more widely conserved role in epigenetic memory.
IL-33 and MRGPRX2-Triggered Activation of Human Skin Mast Cells—Elimination of Receptor Expression on Chronic Exposure, but Reinforced Degranulation on Acute Priming
Authors: Wang Zhao , Guhl Sven , Franke Kristin , Artuc Metin , Zuberbier Torsten , Babina Magda
Published: April 2019

Clinically relevant exocytosis of mast cell (MC) mediators can be triggered by high-affinity IgE receptor (FcεRI)-aggregation (allergic route) or by the so-called pseudo-allergic pathway elicited via MAS-related G protein-coupled receptor-X2 (MRGPRX2). The latter is activated by drugs and endogenous neuropeptides. We recently reported that FcεRI-triggered degranulation is attenuated when human skin mast cells are chronically exposed to IL-33. Here, we were interested in the regulation of the MRGPRX2-route. Chronic exposure of skin MCs to IL-33 basically eliminated the pseudo-allergic/neurogenic route as a result of massive MRGPRX2 reduction. This downregulation seemed to partially require c-Jun N-terminal Kinase (JNK), but not p38, the two kinases activated by IL-33 in skin MCs. Surprisingly, however, JNK had a positive effect on MRGPRX2 expression in the absence of IL-33. This was evidenced by Accell®-mediated JNK knockdown and JNK inhibition. In stark contrast to the dampening effect upon prolonged exposure, IL-33 was able to prime for increased degranulation by MRGPRX2 ligands when administered directly before stimulation. This supportive effect depended on p38, but not on JNK activity. Our data reinforce the concept that exposure length dictates whether IL-33 will enhance or attenuate secretion. IL-33 is, thus, the first factor to acutely enhance MRGPRX2-triggered degranulation. Finally, we reveal that p38, rarely associated with MC degranulation, can positively affect exocytosis in a context-dependent manner.
The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
Authors: Terry Marta I. , Pérez-Sanz Fernando , Díaz-Galián M. Victoria , Pérez de los Cobos Felipe , Navarro Pedro J. , Egea-Cortines Marcos , Weiss Julia
Published: April 2019

The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down CHANEL (PhCHL), the ZEITLUPE ortholog in petunia (PhCHL) by hairpin RNAs. Plants with low PhCHL mRNA had overall decreased size. Growth evaluation using time lapse image analysis showed that early leaf movement was not affected by RNAi:PhCHL, but flower angle movement was modified, moving earlier during the day in knockdown plants than in wild types. Despite differences in stem length, growth rate was not significantly affected by loss of PhCHL. In contrast, petal growth displayed lower growth rate in RNAi:PhCHL. Decreased levels of PhCHL caused strongly modified scent profiles, including changes in composition and timing of emission resulting in volatile profiles highly divergent from the wild type. Our results show a role of PhCHL in controlling growth and development of vegetative and reproductive organs in petunia. The different effects of PhCHL on organ development indicate an organ-specific interpretation of the down regulation of PhCHL. Through the control of both timing and quantitative volatile emissions, PhCHL appears to be a major coordinator of scent profiles.
YAP, ΔNp63, and β-Catenin Signaling Pathways Are Involved in the Modulation of Corneal Epithelial Stem Cell Phenotype Induced by Substrate Stiffness
Authors: Gouveia Ricardo M. , Vajda Flora , Wibowo Jason A. , Figueiredo Francisco , Connon Che J.
Published: April 2019

Recent studies have established that the phenotype of epithelial stem cells residing in the corneal periphery (the limbus) depends on this niche’s distinct biomechanical properties. However, the signaling pathways underlying this dependency are still poorly understood. To address this issue, we investigated the effect of substrate stiffness on the migration, proliferation, and molecular phenotype of human limbal epithelial stem cells (LESCs). Specifically, we demonstrated that cells grown on collagen-based substrates with limbus-like compliance showed higher proliferation and stratification and lower migration capabilities, as well as higher levels of pro-proliferative markers Ki67 and β-Catenin, and LESC markers ΔNp63, ABCG2, and CK15. In contrast, cells on stiffer substrates lost these stem/progenitor cell markers, but instead expressed the key mechanotransduction factor YAP, as well as elevated levels of BMP4, a promotor of cell differentiation known to be negatively regulated by Wnt/β-Catenin signaling. This data allowed us to propose a new model that integrates the various molecular pathways involved in LESC response to substrate stiffness. This model will potentially be a useful guide to future research on the mechanisms underlying LESC loss following fibrosis-causing injuries.
Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model
Authors: Manai Federico , Azzalin Alberto , Morandi Martina , Riccardi Veronica , Zanoletti Lisa , Dei Giudici Marco , Gabriele Fabio , Martinelli Carolina , Bozzola Mauro , Comincini Sergio
Published: April 2019

Celiac disease (CD) is a chronic systemic autoimmune disorder that is triggered by the ingestion of gliadin peptides, the alcohol-soluble fraction of wheat gluten. These peptides, which play a key role in the immune response that underlies CD, spontaneously form aggregates and exert a direct toxic action on cells due to the increase in the reactive oxygen species (ROS) levels. Furthermore, peptic-tryptic digested gliadin peptides (PT-gliadin) lead to an impairment in the autophagy pathway in an in vitro model based on Caco-2 cells. Considering these premises, in this study we have analyzed different mTOR-independent inducers, reporting that the disaccharide trehalose, a mTOR-independent autophagy activator, rescued the autophagy flux in Caco-2 cells treated with digested gliadin, as well as improved cell viability. Moreover, trehalose administration to Caco-2 cells in presence of digested gliadin reduced the intracellular levels of these toxic peptides. Altogether, these results showed the beneficial effects of trehalose in a CD in vitro model as well as underlining autophagy as a molecular pathway whose modulation might be promising in counteracting PT-gliadin cytotoxicity.
p190RhoGAPs, the ARHGAP35- and ARHGAP5-Encoded Proteins, in Health and Disease
Authors: Héraud Capucine , Pinault Mathilde , Lagrée Valérie , Moreau Violaine
Published: April 2019

Small guanosine triphosphatases (GTPases) gathered in the Rat sarcoma (Ras) superfamily represent a large family of proteins involved in several key cellular mechanisms. Within the Ras superfamily, the Ras homolog (Rho) family is specialized in the regulation of actin cytoskeleton-based mechanisms. These proteins switch between an active and an inactive state, resulting in subsequent inhibiting or activating downstream signals, leading finally to regulation of actin-based processes. The On/Off status of Rho GTPases implicates two subsets of regulators: GEFs (guanine nucleotide exchange factors), which favor the active GTP (guanosine triphosphate) status of the GTPase and GAPs (GTPase activating proteins), which inhibit the GTPase by enhancing the GTP hydrolysis. In humans, the 20 identified Rho GTPases are regulated by over 70 GAP proteins suggesting a complex, but well-defined, spatio-temporal implication of these GAPs. Among the quite large number of RhoGAPs, we focus on p190RhoGAP, which is known as the main negative regulator of RhoA, but not exclusively. Two isoforms, p190A and p190B, are encoded by ARHGAP35 and ARHGAP5 genes, respectively. We describe here the function of each of these isoforms in physiological processes and sum up findings on their role in pathological conditions such as neurological disorders and cancers.
Recent Advances in Origins of Life Research by Biophysicists in Japan
Authors: Jia Tony Z. , Kuruma Yutetsu
Published: April 2019

Biophysics research tends to focus on utilizing multidisciplinary technologies and interdisciplinary collaborations to study biological phenomena through the lens of chemistry and physics. Although most current biophysics work is focused on studying extant biology, the fact remains that modern biological systems at some point were descended from a universal common ancestor. At the core of modern biology is the important question of how the earliest life on (or off) Earth emerged. Recent technological and methodological advances developed by biophysicists in Japan have allowed researchers to gain a new suite of knowledge related to the origins of life (OoL). Using these reports as inspiration, here, we highlight some of the significant OoL advances contributed by members of the biophysical research field in Japan with respect to the synthesis and assembly of biological (or pre-biological) components on early Earth, the co-assembly of primitive compartments with biopolymer systems, and the evolution of early genetic systems. We hope to provide inspiration to other biophysicists to not only use the always-advancing suite of available multidisciplinary technologies to continue their own line of work, but to also consider how their work or techniques can contribute to the ever-growing field of OoL research.
Antimicrobial Activities of Hydrophobically Modified Poly(Acrylate) Films and Their Complexes with Different Chain Length Cationic Surfactants
Authors: Gîfu Ioana Cătălina , Maxim Monica Elisabeta , Cinteza Ludmila Otilia , Popa Marcela , Aricov Ludmila , Leontieș Anca Ruxandra , Anastasescu Mihai , Anghel Dan-Florin , Ianchis Raluca , Ninciuleanu Claudia Mihaela , Burlacu Sabina Georgiana , Nistor Cristina Lavinia , Petcu Cristian
Published: April 2019

Multilayer films from hydrophobically modified poly(acrylic acid) (HMPA) and their complexes with cationic surfactants were successfully prepared using the layer-by-layer (LbL) method. Alkyl trimethylammonium bromide derivatives with various lengths of the hydrophobic chain (C10–C18) were used to interact with the HMPA polymer, generating highly hydrophobic domains in the films and contributing to the antimicrobial properties of the prepared coating. The antimicrobial efficiency against common pathogens such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans was investigated in relationship with the morphology and composition of the thin films. The wettability and roughness of the multilayered systems were evaluated using atomic force microscopy (AFM) and contact angle measurements. The effects of the microbial exposure on the surface properties of the prepared films were investigated in order to assess the stability of the HMPA-deposited multilayers and the durability of the antimicrobial activity. The hydrophobically modified films exhibited antimicrobial activity against the studied pathogens. The best efficiency was registered in the case of S. aureus, which showed an inhibition of growth up to 100% after 2 h. C. albicans proved to be less sensitive to the effect of the multilayers deposited from HMPA–surfactant complexes. These results suggest that HMPA and HMPA–surfactant complex LbL multilayer films can be used as promising materials in antimicrobial surface coatings with increased resistance to pathogens during exposure.
Self-Assembled Monolayers on Highly Porous Low-k Dielectrics by 3-Aminopropyltrimethoxysilane Treatment
Authors: Cheng Yi-Lung , Haung Chiao-Wei , Lee Chih-Yen , Chen Giin-Shan , Fang Jau-Shiung
Published: April 2019

Highly porous low-dielectric-constant (low-k) dielectric materials with a dielectric constant (k) less than 2.50 are needed for 32 nm and beyond technological nodes. In this study, a highly porous low-k dielectric film with a k value of 2.25, open porosity of 32.0%, and pore diameter of 1.15 nm were treated by 3-Aminopropyltrimethoxysilane (APTMS) in wet solution in order to form self-assembled monolayers (SAMs) onto it. The effects of the formation SAMs on the electrical characteristics and reliability of highly porous low-k dielectric films were characterized. As SAMs were formed onto the highly porous low-k dielectric film by APTMS treatment, the dielectric breakdown field and the failure time were significantly improved, but at the expense of the increases in the dielectric constant and leakage current. Moreover, the formation SAMs enhanced the Cu barrier performance for highly porous low-k dielectric films. Therefore, the SAMs derived from APTMS treatment are promising for highly porous low-k dielectric films to ensure better integrity.
PVB/ATO Nanocomposites for Glass Coating Applications: Effects of Nanoparticles on the PVB Matrix
Authors: Pizzanelli Silvia , Forte Claudia , Bronco Simona , Guazzini Tommaso , Serraglini Chiara , Calucci Lucia
Published: April 2019

Films made of poly(vinyl butyral) (PVB) and antimony-doped tin oxide (ATO) nanoparticles (NPs), both uncoated and surface-modified with an alkoxysilane, were prepared by solution casting at filler volume fractions ranging from 0.08% to 4.5%. The films were characterized by standard techniques including transmission electron microscopy, thermogravimetric analysis and differential scanning calorimetry (DSC). In the polymeric matrix, the primary NPs (diameter ~10 nm) aggregate exhibiting different morphologies depending on the presence of the surface coating. Coated ATO NPs form spherical particles (with a diameter of 300–500 nm), whereas more elongated fractal structures (with a thickness of ~250 nm and length of tens of micrometers) are formed by uncoated NPs. The fraction of the polymer interacting with the NPs is always negligible. In agreement with this finding, DSC data did not reveal any rigid interface and 1H time domain nuclear magnetic resonance (NMR) and fast field-cycling NMR did not show significant differences in polymer dynamics among the different samples. The ultraviolet-visible-near infrared (UV-Vis-NIR) transmittance of the films decreased compared to pure PVB, especially in the NIR range. The solar direct transmittance and the light transmittance were extracted from the spectra according to CEN EN 410/2011 in order to test the performance of our films as plastic layers in laminated glass for glazing.
A Novel Liquid Packaging Structure of Deep-Ultraviolet Light-Emitting Diodes to Enhance the Light-Extraction Efficiency
Authors: Kang Chieh-Yu , Lin Chih-Hao , Wu Tingzhu , Lee Po-Tsung , Chen Zhong , Kuo Hao-Chung
Published: April 2019

To realize high-efficiency, AlGaN-based, deep-ultraviolet light-emitting diodes (DUV-LEDs), enhancing their light-extraction efficiency and reducing thermal resistance is very crucial. We proposed a liquid packaging structure that could enhance optical power by 27.2% and 70.7% for flat type and lens type 281-nm DUV-LEDs, respectively. A significant improvement effect at different wavelengths, such as 268 nm and 310 nm, was also observed. Furthermore, using the liquid packaging structure, the thermal resistance was reduced by 30.3% compared to the conventional structure. Finally, the reliability of liquid packaging DUV-LEDs was tested. The light output maintenance of liquid packaging DUV-LEDs was compared to the conventional structure.
Passivation Mechanism of Nitrogen in ZnO under Different Oxygen Ambience
Authors: Chen Xingyou , Zhang Zhenzhong , Zhang Yunyan , Yao Bin , Li Binghui , Gong Qian
Published: April 2019

Nitrogen-doped ZnO thin films were grown on a-plane Al2O3 by plasma-assisted molecular beam epitaxy. Hall-effect measurements indicated that the nitrogen-doped ZnO films showed p-type behavior first, then n-type, with the growth conditions changing from oxygen-radical-rich to oxygen-radical-deficient ambience, accompanied with the increase of the N/O ratio in the plasmas. The increasing green emission in the low temperature photoluminescence spectra, related to single ionized oxygen vacancy in ZnO, was ascribed to the decrease of active oxygen atoms in the precursor plasmas. CN complex, a donor defect with low formation energy, was demonstrated to be easily introduced into ZnO under O-radical-deficient ambience, which compensated the nitrogen-related acceptor, along with the oxygen vacancy.
Kinetics of Non-Isothermal and Isothermal Crystallization in a Liquid Crystal with Highly Ordered Smectic Phase as Reflected by Differential Scanning Calorimetry, Polarized Optical Microscopy and Broadband Dielectric Spectroscopy
Authors: Jasiurkowska-Delaporte Małgorzata , Rozwadowski Tomasz , Juszyńska-Gałązka Ewa
Published: April 2019

The kinetics of the non-isothermal and isothermal crystallization of the crystalline smectic B phase (soft crystal B, SmBcr) in 4-n-butyloxybenzylidene-4′-n′-octylaniline (BBOA) was studied by a combination of differential scanning calorimetry (DSC), broadband dielectric spectroscopy (BDS) and polarized optical microscopy (POM). On cooling, part of the SmBcr phase undergoes conversion to a crystalline phase and the remainder forms a glassy state; after the glass softens, crystallization is completed during subsequent heating. By analyzing the area of the crystal growing in the texture of SmBcr as a function of time, the evolution of degree of crystallinity, D(t), was estimated. It was demonstrated that upon heating, D(t) follows the same Avrami curve as the crystallization during cooling. Non-isothermal crystallization observed during slow cooling rates (3K/min ≤ ϕ ≤ 5K/min) is a thermodynamically-controlled process with the energy barrier Ea ≈ 175 kJ/mol; however, the crystallization occurring during fast cooling (5 K/min > ϕ ≥ 30K/min) is driven by a diffusion mechanism, and is characterized by Ea ≈ 305 kJ/mol. The isothermal crystallization taking place in the temperature range 274 K and 281 K is determined by nucleus formation.
Polyphenols Inhibit Candida albicans and Streptococcus mutans Biofilm Formation
Authors: Farkash Yosi , Feldman Mark , Ginsburg Isaac , Steinberg Doron , Shalish Miriam
Published: April 2019

Background: Streptococcus mutans (S. mutans) and Candida albicans (C. albicans) are two major contributors to dental caries. They have a symbiotic relationship, allowing them to create an enhanced biofilm. Our goal was to examine whether two natural polyphenols (Padma hepaten (PH) and a polyphenol extraction from green tea (PPFGT)) could inhibit the caries-inducing properties of S. mutans and C. albicans. Methods: Co-species biofilms of S. mutans and C. albicans were grown in the presence of PH and PPFGT. Biofilm formation was tested spectrophotometrically. Exopolysaccharides (EPS) secretion was quantified using confocal scanning laser microscopy. Biofilm development was also tested on orthodontic surfaces (Essix) to assess biofilm inhibition ability on such an orthodontic appliance. Results: PPFGT and PH dose-dependently inhibited biofilm formation without affecting the planktonic growth. We found a significant reduction in biofilm total biomass using 0.625 mg/mL PPFGT and 0.16 mg/mL PH. A concentration of 0.31 mg/mL PPFGT and 0.16 mg/mL PH inhibited the total cell growth by 54% and EPS secretion by 81%. A reduction in biofilm formation and EPS secretion was also observed on orthodontic PVC surfaces. Conclusions: The polyphenolic extractions PPFGT and PH have an inhibitory effect on S. mutans and C. albicans biofilm formation and EPS secretion.
Supervisors’ Visual Attention Allocation Modeling Using Hybrid Entropy
Authors: Bao Haifeng , Fang Weining , Guo Beiyuan , Wang Peng
Published: April 2019

With the improvement in automation technology, humans have now become supervisors of the complicated control systems that monitor the informative human–machine interface. Analyzing the visual attention allocation behaviors of supervisors is essential for the design and evaluation of the interface. Supervisors tend to pay attention to visual sections with information with more fuzziness, which makes themselves have a higher mental entropy. Supervisors tend to focus on the important information in the interface. In this paper, the fuzziness tendency is described by the probability of correct evaluation of the visual sections using hybrid entropy. The importance tendency is defined by the proposed value priority function. The function is based on the definition of the amount of information using the membership degrees of the importance. By combining these two cognitive tendencies, the informative top-down visual attention allocation mechanism was revealed, and the supervisors’ visual attention allocation model was built. The Building Automatic System (BAS) was used to monitor the environmental equipment in a subway, which is a typical informative human–machine interface. An experiment using the BAS simulator was conducted to verify the model. The results showed that the supervisor’s attention behavior was in good agreement with the proposed model. The effectiveness and comparison with the current models were also discussed. The proposed attention allocation model is effective and reasonable, which is promising for use in behavior analysis, cognitive optimization, and industrial design.
Education—Migration Nexus: Understanding Youth Migration in Southern Ethiopia
Authors: Semela Tesfaye , Cochrane Logan
Published: April 2019

The purpose of this study is to unravel the education–migration nexus in the African context, specifically Ethiopia. It examines why young people terminate their education to migrate out of the country. The study applies de Haas’ aspiration—capability framework and Turner’s macro, meso and micro sociology as its analytical lenses. It offers unique insight into the terrain of youth migration in southern Ethiopia based on empirical data obtained from two rural sub-districts known for high levels of youth out-migration. Data are generated based on interviews with would-be migrant youth, parents, teachers and school principals. The findings reveal that education has both direct and indirect impacts on youth migration. On the other hand, the results indicate that though terminating school could have negative ramifications on human capital accumulation at micro and macro levels, migration can positively impact households and local communities through investments made by individual migrants, migrant-returnees, and remittance-receiving households in small businesses or community development projects, which included better resourced schools.
Passive Multiple Target Indoor Localization Based on Joint Interference Cancellation in an RFID System
Authors: Liu Meng , Ma Liang , Wang Na , Zhang Yajun , Yang Yang , Wang Hongjun
Published: April 2019

Radio frequency identification (RFID) provides a simple and effective solution to the passive indoor localization. The conventional wisdom about RFID localization is using reference tags. It performs well in tag or passive single target localization. However, in the passive multiple target scenario, reference tag based localization suffers from some limitations, including the array aperture, mutual coupling of reference tags, and coherent superimposition signals. These problems are harmless and ignored in tag or passive single target localization, but degrade the performance severely in passive multiple target scenario. Therefore, in this paper, the authors propose a joint interference cancellation method to mitigate the effect of these limitations. Uniform circular array (UCA) of reference tags were used to reduce the interference of the array aperture. A carefully designed relative position of adjacent reference tags and a modified channel model were combined to reduce the mutual coupling. A virtual distributed reader antenna array was used to reduce the false positive and false negative estimations. The system was evaluated in real indoor environment using noodles and colas as targets and can work well in a smoky environment that is similar to some real industrial environments. The accuracy of target number estimation is 97.5%. The spatial resolution is about 30 cm, and the median error of 2-D multiple target localization is about 5.5 cm.
AgreeRelTrust—A Simple Implicit Trust Inference Model for Memory-Based Collaborative Filtering Recommendation Systems
Authors: Zahir Ahmed , Yuan Yuyu , Moniz Krishna
Published: April 2019

Recommendation systems alleviate the problem of information overload by helping users find information relevant to their preference. Memory-based recommender systems use correlation-based similarity to measure the common interest among users. The trust between users is often used to address the issues associated with correlation-based similarity measures. However, in most applications, the trust relationships between users are not available. A popular method to extract the implicit trust relationship between users employs prediction accuracy. This method has several problems such as high computational cost and data sparsity. In this paper, addressing the problems associated with prediction accuracy-based trust extraction methods, we proposed a novel trust-based method called AgreeRelTrust. Unlike accuracy-based methods, this method does not require the calculation of initial prediction and the trust relationship is more meaningful. The collective agreements between any two users and their relative activities are fused to obtain the trust relationship. To evaluate the usefulness of our method, we applied it to three public data sets and compared the prediction accuracy with well-known collaborative filtering methods. The experimental results show our method has large improvements over the other methods.
Open-Source Electronics Platforms: Development and Applications
Authors: Ngo Trung Dung
Published: April 2019

Open-source electronics are becoming very popular with our daily educational and developmental purposes [...]
An Experimental Investigation of Flow Regimes in Imbibition and Drainage Using a Microfluidic Platform
Authors: Guo Feng , Aryana Saman A.
Published: April 2019

Instabilities in immiscible displacement along fluid−fluid displacement fronts in porous media are undesirable in many natural and engineered displacement processes such as geological carbon sequestration and enhanced oil recovery. In this study, a series of immiscible displacement experiments are conducted using a microfluidic platform across a wide range of capillary numbers and viscosity ratios. The microfluidic device features a water-wet porous medium, which is a two-dimensional representation of a Berea sandstone. Data is captured using a high-resolution camera, enabling visualization of the entire domain, while being able to resolve features as small as 10 µm. The study reports a correlation between fractal dimensions of displacement fronts and displacement front patterns in the medium. Results are mapped on a two-dimensional parameter space of log M and log Ca, and stability diagrams proposed in literature for drainage processes are superimposed for comparison. Compared to recent reports in the literature, the results in this work suggest that transition regimes may constitute a slightly larger portion of the overall flow regime diagram. This two-phase immiscible displacement study helps elucidate macroscopic processes at the continuum scale and provides insights relevant to enhanced oil recovery processes and the design of engineered porous media such as exchange columns and membranes.
Innovative Policies for Energy Efficiency and the Use of Renewables in Households
Authors: Strielkowski Wadim , Volkova Elena , Pushkareva Luidmila , Streimikiene Dalia
Published: April 2019

Renewable energy sources (RES) are gradually becoming one of the key elements in the process of achieving energy efficiency worldwide. This trend can be observed in many developed Western economies—for example, in the United States, as well as in the United Kingdom. Hence, the role of innovative policies for promoting energy efficiency is becoming crucial in transition to the post-carbon economy. The shift to the carbon-free future make all actors to face forgoing commitments Nevertheless, customers and residential households are the first and the most important players in the pursuit of the energy-efficient future. Without them, carbon-free economy based on RES would never take the shape as envisaged. Our paper focuses on the innovative strategies and policies studying the effect and the scope of RES penetration into the households. We employ and empirical analysis of the effects from using RES in households using an example of the residential households in the northwest region of the United Kingdom (UK) with and without solar photovoltaic (PV) panels and electric vehicles (EV). We analyse the four scenarios that are aimed at analysing the system dynamics and providing differentiation between systems in terms of the varying values of the gross demand, tariffs, metered import, and the total revenue. Our results demonstrate that the solar PV leads to the transfer of costs and wealth regardless of the ownership of PV and EVs. Solar energy generation reduces the share of UK solar PV households per kWh costs of the distribution system which causes the augmenting of the per unit charges as well as to the changes in payments for the electricity that impoverishes less wealthy customer groups. It also becomes clear that with the increase of EV penetration, the existing energy efficiency schemes would have to be revised.
Three Vectors Model Predictive Torque Control Without Weighting Factor Based on Electromagnetic Torque Feedback Compensation
Authors: Li Haixia , Lin Jican , Lu Ziguang
Published: April 2019

Finite control set-model predictive torque control (FCS-MPTC) depends on the system parameters and the weight coefficients setting. At the same time, since the actual load disturbance is unavoidable, the model parameters are not matched, and there is a torque tracking error. In traditional FCS-MPTC, the outer loop—that is, the speed loop—adopts a classic Proportional Integral (PI) controller, abbreviated as PI-MPTC. The pole placement of the PI controller is usually designed by a plunge-and-test, and it is difficult to achieve optimal dynamic performance and optimal suppression of concentrated disturbances at the same time. Aiming at squirrel cage induction motors, this paper first proposes an outer-loop F-ETFC-MPTC control strategy based on a feed-forward factor for electromagnetic torque feedback compensation (F-ETFC). The electromagnetic torque was imported to the input of the current regulator, which is used as the control input signal of feedback compensation of the speed loop; therefore, the capacity of an anti-load-torque-disturbance of the speed loop was improved. The given speed is quantified by a feed-forward factor into the input of the current regulator, which is used as the feed-forward adjustment control input of the speed controller to improve the dynamic response of the speed loop. The range of the feed-forward factor and feed-back compensation coefficient can be obtained according to the structural analysis of the system, which simplifies the process of parameter design adjustment. At the same time, the multi-objective optimization based on the sorting method replaces the single cost function in traditional control, so that the selection of the voltage vector works without the weight coefficient and can solve complicated calculation problems in traditional control. Finally, according to the relationship between the voltage vector and the switch state, the virtual six groups of three vector voltages can be adjusted in both the direction and amplitude, thereby effectively improving the control performance and reducing the flow rate and torque ripple. The experiment is based on the dSPACE platform, and experimental results verify the feasibility of the proposed F-ETFC-MPTC. Compared with traditional PI-MPTC, the feed-forward factor can effectively improve the stability time of the system by more than 10 percent, electromagnetic torque feedback compensation can improve the anti-load torque disturbance ability of the system by more than 60 percent, and the three-vector voltage method can effectively reduce the disturbance.
LCOE Analysis of Tower Concentrating Solar Power Plants Using Different Molten-Salts for Thermal Energy Storage in China
Authors: Zhuang Xiaoru , Xu Xinhai , Liu Wenrui , Xu Wenfu
Published: April 2019

In recent years, the Chinese government has vigorously promoted the development of concentrating solar power (CSP) technology. For the commercialization of CSP technology, economically competitive costs of electricity generation is one of the major obstacles. However, studies of electricity generation cost analysis for CSP systems in China, particularly for the tower systems, are quite limited. This paper conducts an economic analysis by applying a levelized cost of electricity (LCOE) model for 100 MW tower CSP plants in five locations in China with four different molten-salts for thermal energy storage (TES). The results show that it is inappropriate to build a tower CSP plant nearby Shenzhen and Shanghai. The solar salt (NaNO3-KNO3, 60-40 wt.%) has lower LCOE than the other three new molten-salts. In order to calculate the time when the grid parity would be reached, four scenarios for CSP development roadmap proposed by International Energy Agency (IEA) were considered in this study. It was found that the LCOE of tower CSP would reach the grid parity in the years of 2038–2041 in the case of no future penalties for the CO2 emissions. This study can provide support information for the Chinese government to formulate incentive policies for the CSP industry.