bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of brown and green seaweeds on diet digestibility, ruminal fermentation patterns and enteric methane emissions using the rumen simulation technique
Authors: Roskam Emily , Kirwan Stuart F. , Kenny David A. , O’Donnell Caroline , O’Flaherty Vincent , Hayes Maria , Waters Sinéad M.
Published: December 2022

Inclusion of the red seaweed Asparagopsis taxiformis as a feed additive, has led to significant reductions in methane (CH4) production from ruminants. However, dietary supplementation with this seaweed is negatively associated with health and environmental concerns mainly due to its bromoform content, a compound with potential carcinogenic properties. Thus, there is renewed focus on ascertaining the anti-methanogenic potential of locally grown brown and green seaweeds, which typically do not contain bromoform. The objective of this study was to investigate the effects of selected brown and green seaweeds on diet digestibility, ruminal fermentation patterns, total gas (TGP) and CH4 production in vitro, using the rumen simulation technique system. In experiment 1, Pelvetia canaliculata (PEC) was examined. In experiment 2, Cystoseira tamariscifolia (CYT), Bifurcaria bifurcata (BIB), Fucus vesiculosus (FUV), Himanthalia elongata (HIM) and Ulva intestinalis (ULI) were analysed. Ascophyllum nodosum (ASC) was included in both experiments. A diet containing A. taxiformis (ASP1; ASP2) and an unsupplemented diet (CON) were included as positive and negative controls, respectively in both experiments. All seaweeds were included at a rate of 10 g/kg dry matter (DM) into a control diet of 50:50 (w:w) forage:concentrate. The seven brown and green seaweeds assessed failed to affect absolute CH4 emissions or alter fermentation patterns. In experiment 1, seaweed treatment had no effect on diet digestibility, CH4%, CH4 mmol/d or CH4 L/d (P>0.1), however ASP1 reduced CH4 mmol/g DOM by 49% (P<0.01) relative to the control. Both ASC and ASP1 tended to increase TGP (P<0.1) relative to the control. In addition to this, the inclusion of seaweed in experiment 1 reduced the production of NH3-N (P<.0001) compared to the control. In experiment 2, seaweed treatment had no effect on diet digestibility or TGP. Both ASP2 and FUV reduced CH4% (P<0.01) but only ASP2 significantly reduced CH4 mmol/d, CH4 L/d and CH4 mmol/g DOM (P<0.05). Daily mMol butyrate was reduced by ASP2 relative to the control and most other seaweeds (P<.0001). In both experiment 1 and 2, seaweed inclusion had no effect on daily total VFA, acetate or propionate production or the acetate:propionate ratio relative to the control. To conclude, including the bromoform-free brown and green seaweeds at 10g/kg DM has no negative effects on diet digestibility or fermentation patterns but also failed to reduce the production of enteric CH4in vitro.
How β-cyclodextrin- loaded mesoporous SiO2 nanospheres ensure efficient adsorption of rifampicin
Authors: Sun Xun , Chen Mingming , Lei Jiayu , Liu Xinran , Ke Xin , Liu Wengang , Wang Jingkuan , Gao Xiaodan , Liu Xin , Zhang Yun
Published: December 2022

In this study, β-CD@mesoporous SiO2 nanospheres (β-CD@mSi) were prepared by loading β-cyclodextrin (β-CD) onto mesoporous silica nanospheres through an in situ synthesis. This not only solved the defect of β-CD being easily soluble in water, but also changed the physical structure of the mesoporous silica nanospheres. FTIR and XPS results showed that β-CD was successfully loaded onto mesoporous silica nanospheres (mSi), while enhancing the adsorption effect. β-CD@mSi with a monomer diameter of about 150 nm were prepared. At a temperature of 298k, the removal efficiency of a 100 mg/L solution of rifampicin can reach 90% in 4 h and the adsorption capacity was 275.42 mg g−1 at high concentration. Through the calculation and analysis of adsorption kinetics, adsorption isotherms and adsorption thermodynamics based on the experimental data, the reaction is a spontaneous endothermic reaction dominated by chemical adsorption. The electron transfer pathway, structure–activity relationship and energy between β-CD@mSi and rifampicin were investigated by quantum chemical calculations. The accuracy of the characterization test results to judge the adsorption mechanism was verified, to show the process of rifampicin removal by β-CD@mSi more clearly and convincingly. The simulation results show that π–π interaction plays a major interaction in the reaction process, followed by intermolecular hydrogen bonding and electrostatic interactions.
Evolution of magnetic properties in iron-based superconductor Eu-doped CaFe2As2
Authors: Shrestha K. , Deng L. Z. , Lv B. , Chu C. W.
Published: December 2022

This work presents the evolution of magnetic properties of EuxCa1−xFe2As2 (0 ≤ x ≤ 1, ECFA) samples. Unlike the resistivity data, that for magnetic susceptibility χ (T) does not show any clear evidence of the spin density wave (SDW) transition. When the Curie-Weiss contribution is subtracted, a weak anomaly appears at a temperature close to the SDW transition temperature (TSDW) determined from the resistivity data. To understand the magnetic orders arising from Fe-moments and Eu2+ spins order, we have studied the doping dependence of TSDW and Eu2+ antiferromagnetic order TN. It is found that TSDW increases almost linearly with increasing x and remains nearly unchanged above x ∼ 0.4, whereas TN first appears at x ∼ 0.4 and varies almost linearly with further increasing x. These observations suggest that magnetic orders due to two sublattices are coupled to each other. The results discussed here are helpful for understanding the magnetic properties of ECFA and other iron-based superconductors.
Comparison of green building policies and regulations between central and local governments of China: Analysis based on text similarity
Authors: Hu Yuan , Lin Yajing
Published: December 2022

The development of green buildings, as the focus of the construction field, is an inevitable choice to achieve the goal of carbon peaking and carbon neutralization. From the perspective of text similarity, this paper conducts a comparative study on the 14th Five-Year Plan for Building Energy Conservation and Green Building Development issued by the Ministry of Housing and Urban-Rural Development of the PRC in 2022 and sixteen local regulations on green buildings at provincial levels. The results show that: First, promoting energy-saving and green transformation of existing public buildings, promoting new green construction methods, strengthening green building management system construction and other contents, have the highest similarity value, indicating that these three contents have the highest overall notices in the legislation formulation level of green buildings in different regions; Second, the legal texts formulated by Tianjin and Shanghai are more in line with the requirements of the 14th Five-Year Plan than those of other provinces, showing the forward-looking nature of local legislation; Lastly, through data analysis, it is found that the average similarities of the three deployments of green buildings have small differences, but there are large differences between regions. Jiangxi, Qinghai and Guangdong have some outstanding contents, forming regional characteristics according to local conditions, showing the differences of local legal texts. Since the Plan is the latest national guidance, it requires local regulations to refine and implement the contents of the Plan. Based on the comprehensive comparative analysis of the text, it is recommended that all localities should check and fill gaps according to the Plan, improve the key task systems, and use local standards to build legal guarantee tailored to local conditions.
Mutation spectrum of PTS gene in patients with tetrahydrobiopterin deficiency from jiangxi province
Authors: Xie Kang , Zeng Baitao , Zhang Liuyang , Chen Shaohong , Zou Yongyi , Yuan Huizhen , Huang Shuhui , Wang Feng , Lu Qing , Liu Yanqiu , Yang Bicheng
Published: December 2022

Background: Hyperphenylalaninemia (HPA) is the most common inborn error in amino acid metabolism. It can be primarily classified into phenylalanine hydroxylase (PAH) deficiency and tetrahydrobiopterin (BH4) deficiency. BH4 deficiency (BH4D) is caused by genetic defects in enzymes involved in the biosynthesis and regeneration of BH4. 6-pyruvoyl-tetrahydropterin synthase (PTPS/PTS), which is encoded by the PTS gene, participates in the biosynthesis of BH4. PTPS deficiency (PTPSD) is the major cause of BH4D. In this study, we investigated that the prevalence of BH4D in Jiangxi province was approximately 12.5 per 1,000,000 live births (69/5,541,627). Furthermore, the frequency of BH4D was estimated to be 28.8% (69/240) in the HPA population of Jiangxi. In this study, we aimed to characterize the mutational spectrum of the PTS gene in patients with PTPSD from Jiangxi province.

Method: Newborn screening data of Jiangxi province from 1997 to 2021 were analyzed and 53 families with PTPSD were enrolled for the analysis of the PTS gene variants by Sanger sequencing.

Results: 106 variants were identified in 106 alleles of 53 patients with PTPSD, including 13 types of variants reported previously, and two novel variants (c.164-36A>G and c.146_147insTG). The predominant variant was c.259C>T (47.2%), followed by c.84-291A>G (19.8%), c.155A>G (8.5%), c.286G>A (6.6%) and c.379C>T (4.7%).

Conclusion: The results of this study can not only provide guidance for the molecular diagnosis and genetic counseling in cases of PTPS deficiency but also enrich the PTS mutation database.
Optimized riboswitch-regulated AAV vector for VEGF-B gene therapy
Authors: Eriksson Reetta A. E. , Nieminen Tiina , Galibert Lionel , Peltola Sanna K. , Tikkanen Petra , Käyhty Piia , Leinonen Hanna M. , Oruetxebarria Igor , Lepola Saana , Valkama Anniina J. , Lipponen Eevi M. , Lesch Hanna P. , Ylä-Herttuala Seppo , Airenne Kari J.
Published: December 2022

Gene therapy would greatly benefit from a method to regulate therapeutic gene expression temporally. Riboswitches are small RNA elements that have been studied for their potential use in turning transgene expression on or off by ligand binding. We compared several tetracycline and toyocamycin-inducible ON-riboswitches for a drug responsive transgene expression. The tetracycline-dependent K19 riboswitch showed the best control and we successfully applied it to different transgenes. The induction of gene expression was 6- to 10-fold, dose-dependent, reversible, and occurred within hours after the addition of a clinically relevant tetracycline dose, using either plasmid or adeno-associated virus (AAV) vectors. To enhance the switching capacity, we further optimized the gene cassette to control the expression of a potential therapeutic gene for cardiovascular diseases, VEGF-B. Using two or three riboswitches simultaneously reduced leakiness and improved the dynamic range, and a linker sequence between the riboswitches improved their functionality. The riboswitch function was promoter-independent, but a post-transcriptional WPRE element in the expression cassette reduced its functionality. The optimized construct was a dual riboswitch at the 3′ end of the transgene with a 100 bp linker sequence. Our study reveals significant differences in the function of riboswitches and provides important aspects on optimizing expression cassette designs. The findings will benefit further research and development of riboswitches.
Cytotoxic secondary metabolites isolated from Penicillium sp. YT2019-3321, an endophytic fungus derived from Lonicera Japonica
Authors: Weng Wenya , Jiang Shicui , Sun Chuchu , Pan Xiaofu , Xian Li , Lu Xuemian , Zhang Chi
Published: December 2022

Introduction

Endophytic fungi associated with medicinal plants have proven to possess a high potential to produce structurally diverse metabolites, some of which are valuable for medicinal applications. In this study, Penicillium sp. YT2019-3321, an endophytic fungus derived from traditional Chinese medicine Lonicera japonica, was chemically studied.

Methods

The chemical structures of the isolated compounds were established by a correlative interpretation of HRESIMS and NMR spectroscopic data. The optical resolution of (±)-1 by chiral HPLC yielded individual enantiomers (+)-1 and (–)-1, and their stereochemistry were solved by X-ray diffraction crystallography, respectively.

Results and discussion

Eight structurally diversified secondary metabolites, including two previously unreported polyketides, named (±)-chrysoalide B (1) and penicidone E (2), were isolated and identified from Penicillium sp. YT2019-3321. Compound 2 possessed the γ-pyridone nucleus, which is rarely found in natural products. Cytotoxic assay revealed that the new compound 2 demonstrated a dose-dependent cytotoxicity against the human pancreatic tumor cells PATU8988T with the IC50 value of 11.4 μM. Further studies indicated that 2 significantly induced apoptosis of PATU8988T cell lines, characterized by the morphologies abnormity, the reduction of cell number, the upregulation of proportion of apoptotic cells, and the ratio of Bcl-2 to Bax. Our study demonstrates that fungal secondary metabolites may have important significance in the discovery of drug leads.
Evaluation of the non-auditory neurocognitive test MoCA-HI for hearing-impaired
Authors: Völter Christiane , Fricke Hannah , Götze Lisa , Labrenz Franziska , Tokic Marianne , Wirth Rainer , Nasreddine Ziad S. , Dawes Piers
Published: December 2022

Background

Since hearing loss and cognitive decline often co-occur among older adults, a cognitive screening test suitable for hearing-impaired people is of high clinical relevance. We report the first evaluation of a German language version of the Montreal Cognitive Assessment—Hearing Impaired version (MoCA-HI).

Objective

The aim of the present study was to compare cognitively healthy participants with and without hearing loss, to examine the impact of age, sex, educational level and degree of hearing impairment on the German MoCA-HI performance, and to develop normative data.

Material and methods

The German MoCA-HI was tested in 94 participants with normal or mild hearing impairment (group 1: 4PTA ≤ 40 dB on the better hearing ear) and 81 participants with moderate to profound hearing loss (group 2: 4PTA > 40 dB on the better hearing ear). Additionally, all participants performed the standard MoCA (version 8.2).

Results

No significant group difference between group 1 and 2 was found in the MoCA-HI total score (p = 0.05). In contrast, group 1 performed significantly better than group 2 on the standard MoCA (p < 0.001). There was no difference between the MoCA and the MoCA-HI performance in group 1 (p = 0.12), whereas individuals of group 2 performed significantly better on the MoCA-HI than on the standard MoCA (p < 0.001). Test-retest reliability of the MoCA-HI was high (p < 0.001). Higher age (p < 0.001), male sex (p = 0.009) and lower education (p < 0.001) were associated with a lower overall MoCA-HI score. Based on the demographic data normative data were developed by a regression-based approach.

Conclusion

The MoCA-HI is a cognitive screening test which is suitable for people with hearing impairment.
Visualization of both proximal M2-MCA segments in patients (the Tilted-V Sign) with acute M1-MCA occlusion stroke is associated with better procedural and prognostic outcomes
Authors: Azriel Amit , Horev Anat , Avraham Elad , Alguayn Farouq , Zlotnik Yair , Ifergane Gal , Sufaro Yuval Zeev , Dizitzer Yotam , Melamed Israel , Shelef Ilan , Cohen José E. , Leker Ronen R. , Honig Asaf
Published: December 2022

Introduction

We aimed to assess the clinical significance of M1-MCA occlusion with visualization of both MCA-M2 segments [“Tilted-V sign” (TVS)] on initial CT angiography (CTA) in patients with acute ischemic stroke (AIS) undergoing endovascular thrombectomy (EVT).

Methods

Data for patients with consecutive AIS undergoing EVT for large vessel occlusion (LVO) in two academic centers are recorded in ongoing databases. Patients who underwent EVT for M1-MCA occlusions ≤ 6 h from symptom onset were included in this retrospective analysis.

Results

A total of 346 patients met the inclusion criteria; 189 (55%) had positive TVS. Patients with positive TVS were younger (68 ± 14 vs. 71 ± 14 years, P = 0.028), with similar rates of vascular risk factors and baseline modified Rankin scores (mRS) 0–2. The rates of achieving thrombolysis in cerebral ischemia (TICI) 2b-3 were similar to the two groups (79%), although successful first-pass recanalization was more common with TVS (64 vs. 36%, p = 0.01). On multivariate analysis, higher collateral score [odds ratio (OR) 1.38 per unit increase, p = 0.008] and lower age (OR 0.98 per year increase, p = 0.046) were significant predictors of TVS. Patients with positive TVS had higher post-procedural Alberta Stroke Program Early CT Score (ASPECTS; 6.9 ± 2.2 vs. 5.2 ± 2.3, p = 0.001), were discharged with lower National Institutes of Health Stroke Score (NIHSS; 6±6 vs. 9±7, p = 0.003) and higher rates of mRS 0–2 (29.5 vs. 12%, p = 0.001), and had lower rates of 90-day mortality (13.2 vs. 21.6%, p = 0.038). However, TVS was not an independent predictor of functional independence (OR 2.51; 95% CI 0.7–8.3).

Conclusion

Tilted-V Sign, an easily identifiable radiological marker, is associated with fewer recanalization attempts, better functional outcomes, and reduced mortality.
Modeling neuromuscular diseases in zebrafish
Authors: Singh Jaskaran , Patten Shunmoogum A.
Published: December 2022

Neuromuscular diseases are a diverse group of conditions that affect the motor system and present some overlapping as well as distinct clinical manifestations. Although individually rare, the combined prevalence of NMDs is similar to Parkinson’s. Over the past decade, new genetic mutations have been discovered through whole exome/genome sequencing, but the pathogenesis of most NMDs remains largely unexplored. Little information on the molecular mechanism governing the progression and development of NMDs accounts for the continual failure of therapies in clinical trials. Different aspects of the diseases are typically investigated using different models from cells to animals. Zebrafish emerges as an excellent model for studying genetics and pathogenesis and for developing therapeutic interventions for most NMDs. In this review, we describe the generation of different zebrafish genetic models mimicking NMDs and how they are used for drug discovery and therapy development.
Hydroxy-α-sanshool isolated from Zanthoxylum bungeanum Maxim. has antidiabetic effects on high-fat-fed and streptozotocin-treated mice via increasing glycogen synthesis by regulation of PI3K/Akt/GSK-3β/GS signaling
Authors: Zhang Qing , Li Ruo-Lan , Wang Ling-Yu , Zhang Ting , Qian Die , Tang Dan-Dan , He Cheng-Xun , Wu Chun-Jie , Ai Li
Published: December 2022

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by hyperglycemia. The fruits of Zanthoxylum bungeanum Maxim. is a common spice and herbal medicine in China, and hydroxy-α-sanshool (HAS) is the most abundant amide in Z. bungeanum and reported to have significant hypoglycemic effects. The purpose of this study was to evaluate the ameliorative effects of HAS on T2DM and the potential mechanisms responsible for those effects. An acute toxicity test revealed the median lethal dose (LD50) of HAS is 73 mg/kg. C57BL/6 J mice were fed a high-fat diet and given an intraperitoneal injection of streptozotocin (STZ) to induce T2DM in mice to evaluate the hypoglycemic effects of HAS. The results showed that HAS significantly reduced fasting blood glucose, reduced pathological changes in the liver and pancreas, and increased liver glycogen content. In addition, glucosamine (GlcN)-induced HepG2 cells were used to establish an insulin resistance cell model and explore the molecular mechanisms of HAS activity. The results demonstrated that HAS significantly increases glucose uptake and glycogen synthesis in HepG2 cells and activates the PI3K/Akt pathway in GlcN-induced cells, as well as increases GSK-3β phosphorylation, suppresses phosphorylation of glycogen synthase (GS) and increases glycogen synthesis in liver cells. Furthermore, these effects of HAS were blocked by the PI3K inhibitor LY294002. The results of our study suggest that HAS reduces hepatic insulin resistance and increases hepatic glycogen synthesis by activating the PI3K/Akt/GSK-3β/GS signaling pathway.
Experimental study of a tunable perfect flexural wave absorber with a piezoelectric shunted resonator
Authors: Li Xiaopeng , Yu Ziqi , Lee Taehwa
Published: December 2022

Metamaterials and metasurfaces have been widely developed recently for extraordinary acoustic and elastic wave control at a deep subwavelength scale. Perfect wave absorption as an extreme case to totally absorb the impinged waves has gained great attention, whereas most existing designs based on local resonance lack tunabilities, making perfect absorption be observed at a single frequency. To overcome this drawback, in this work, we design and fabricate a tunable inductance-resistance (LR) shunted mechanical resonator via a bonded piezoelectric patch for perfect flexural wave absorption at low frequency. The LR shunted absorber could be reconfigured to a broad frequency range for perfect flexural wave absorption. The tunable perfect absorption performances are validated through experiments and unit absorption is achieved in experiments. In the end, to further highlight the advantages of shunted damping we numerically demonstrate that the absorption spectrum could be enhanced to broadband absorption with a negative capacitance and an inductance-resistance circuit (NC-LR) connected in parallel. The approach proposed provides an alternative solution to achieve perfect wave absorption in the low-frequency range and enables practical application in complex engineering structures.
Research on the identification and detection of field pests in the complex background based on the rotation detection algorithm
Authors: Zhang Wei , Xia Xulu , Zhou Guotao , Du Jianming , Chen Tianjiao , Zhang Zhengyong , Ma Xiangyang
Published: December 2022

As a large agricultural and population country, China’s annual demand for food is significant. The crop yield will be affected by various natural disasters every year, and one of the most important factors affecting crops is the impact of insect pests. The key to solving the problem is to detect, identify and provide feedback in time at the initial stage of the pest. In this paper, according to the pest picture data obtained through the pest detection lamp in the complex natural background and the marking categories of agricultural experts, the pest data set pest rotation detection (PRD21) in different natural environments is constructed. A comparative study of image recognition is carried out through different target detection algorithms. The final experiment proves that the best algorithm for rotation detection improves mean Average Precision by 18.5% compared to the best algorithm for horizontal detection, reaching 78.5%. Regarding Recall, the best rotation detection algorithm runs 94.7%, which is 7.4% higher than horizontal detection. In terms of detection speed, the rotation detection time of a picture is only 0.163s, and the model size is 66.54MB, which can be embedded in mobile devices for fast detection. This experiment proves that rotation detection has a good effect on pests’ detection and recognition rate, which can bring new application value and ideas, provide new methods for plant protection, and improve grain yield.
Applying Apriori algorithm to explore long-term care services usage status—Variables based on the combination of patients with dementia and their caregivers
Authors: Chen Yen-Jen , Jhang Kai-Ming , Wang Wen-Fu , Lin Guan-Cheng , Yen Shao-Wei , Wu Hsin-Hung
Published: December 2022

Purpose

The aim of this study was to identify the combination of patients with dementia and their caregivers' characteristics associated with long-term care (LTC) services usage.

Patients and methods

A cross-sectional study was conducted with 475 patients with mild, moderate, and severe dementia at Changhua Christian Hospital, Taiwan. Eleven types of variables from patients with dementia, nine types of variables from patients' caregivers, and 15 types of LTC services were used for this study. The Apriori algorithm was employed to identify the attributes from the patients and their caregivers who used a particular LTC service from a comprehensive viewpoint.

Results

A total of 75 rules were generated by the Apriori algorithm with support of 2%, confidence of 80%, and lift >1. Among these rules, 25 rules belonged to home personal care services which were summarized further into four general rules for home personal care services. On the other hand, 50 rules belonged to assistive devices that were summarized further into 21 general rules based on their similarities. Patient's walking ability, patient's emotional liability, unemployed or retired caregivers, caregivers' feelings with either helplessness or hopelessness, and caregivers who cared for patients with dementia solely were found to be the critical variables to use home personal care services. In contrast, patient's walking ability, age, and severity as well as caregivers' age, mood, marital status, caregiving burden, and the patient being cared for mainly by a foreign care helper were found to be the critical variables to use assistive devices.

Conclusion

This study showed preliminary results on the LTC service usage from patients with dementia and their caregivers residing in the community. Understanding the patient–caregiver dyad's profile leads the service providers, policymakers, and the referral team to tailor service provisions better to meet the needs and identify the potential target groups. The findings in this study serve as references to reduce caregivers' burden as well as to improve the quality of care for patients with dementia.
Promoting college students’ systems thinking in asynchronous discussions: Encouraging students initiating questions
Authors: Yu Yawen , Chen Gaowei , Tao Yang , Li Xiaofeng , Yang Lina , Dong Shengwen
Published: December 2022

Introduction

Systems thinking is one of the most important thinking skills for medical students. Most of the studies focused on designing technological-rich learning environments which usually take several weeks or months to implement. However, the occurring of COVID-19 health crisis does not allow extensive period of time to implement classroom interventions. How to support students’ systems thinking in fully online environments remains an issue. This study examines if encouraging students initiating questions on asynchronous discussion forum supports their systems thinking development.

Methods

Twenty-two junior students participated in this study. We compared if and how students developed systems thinking when they were encouraged asking questions in asynchronous discussion forums in one unit with another unit in which traditional method was used. Multiple analytical methods were applied in this study, including, social network analysis, epistemic network analysis, inferential statistical analysis and qualitative analysis.

Results

Quantitative results showed that all students improved systems thinking compared with traditional teaching unit among which leader students improved most. Further analysis on students’ discussion posts suggested leader students asked high systems thinking level questions and provided high level responses. Epistemic network analysis unpacked how leader, regular and peripheral students engaged in initiating questions and providing responses differently.

Discussion

This study provides methodological and practical contributions. Methodologically, this study extends prior methods of applying network analysis beyond its original preservice teacher training contexts; practically, this study provides strategies to practitioners to support students’ asynchronous forum discussions.
Disentangling motivation and engagement: Exploring the role of effort in promoting greater conceptual and methodological clarity
Authors: Nagy Robin P. , Martin Andrew J. , Collie Rebecca J.
Published: December 2022

Conflation over motivation and engagement has historically impeded research and practice. One reason for this is because definition and measurement have often been too general or diffuse—especially in the case of engagement. Recently conceptual advances aimed at disentangling facets of engagement and motivation have highlighted a need for better psychometric precision—particularly in the case of engagement. To the extent that engagement is inadequately assessed, motivation research involving engagement continues to be hampered. The present study investigates multidimensional effort (a specific facet of engagement) and how it relates to motivation. In particular, we examine the associations between specific positive and negative motivation factors and dimensions of effort, thereby shedding further insight into how different types of motivation interplay with different types of engagement. Drawing on data from a sample of 946 Australian high school students in 59 mathematics classrooms at five schools, this study hypothesized a tripartite model of academic effort in terms of operative, cognitive, and social–emotional dimensions. A novel nine-item self-report Effort Scale measuring each of the three factors was developed and tested for internal and external validity—including its relationship with multidimensional motivation. Multilevel confirmatory factor analyses were conducted to test the factor structure and validity of multidimensional effort. Additionally, doubly-latent multilevel structural equation models were conducted to explore the hypothesized motivation → engagement (effort) process, and the role of student- and classroom-level background attributes as predictors of both motivation and effort. Results supported the hypothesized model of tripartite effort and its distinctiveness from motivation, and showed that key dimensions of motivation predicted effort at student- and classroom-levels. This study provides implications and suggestions for future motivation research and theorizing by (1) establishing evidence for the validity of a novel engagement framework (multidimensional effort), and (2) supporting future measurement and practice in academic engagement juxtaposed with multidimensional motivation—critical for better understanding engagement, and motivation itself.
Research on the influence of team psychological capital on team members’ work performance
Authors: Jiao Yongji , Zhang Xiaoman , Lu Siwen , Wu Zimo , Deng Yuqian
Published: December 2022

Team psychological capital is the positive psychological state of a team and has a positive impact on the human resource management and performance management of the team. Team members’ work performance, as a component of team performance, has an important impact on improving team performance. However, there is less cross-level impact analysis between the two. The purpose of this paper is to explore the mechanisms of the cross-level effects of team psychological capital on members’ performance. A paired questionnaire survey was administered to 387 human resource management team members from 86 human resource management teams across China, and SPSS 22.0, AMOS 23.0, and HLM 6.08 software were used to analyze the questionnaire data at the same level and across levels. The test shows that the cross-level positive effect of team psychological capital on team members’ work performance is established, and the cross-level mediating role of team members’ psychological capital and team support between the two is established. It enriches the cross-level research from team to individual, refines and enriches the research on the impact of team psychological capital on individual behavior, and provides new ideas for team management in companies. Therefore, when conducting team management, enterprises can enhance team members’ psychological capital by improving team psychological capital, thereby improving team members’ work performance and team efficiency.
Utilising online eye-tracking to discern the impacts of cultural backgrounds on fake and real news decision-making
Authors: Brockinton Amanda , Hirst Sam , Wang Ruijie , McAlaney John , Thompson Shelley
Published: December 2022

Introduction

Online eye-tracking has been used in this study to assess the impacts of different cultural backgrounds on information discernment. An online platform called RealEye allowed participants to engage in the eye-tracking study from their personal computer webcams, allowing for higher ecological validity and a closer replication of social media interaction.

Methods

The study consisted of two parts with a total of five visuals of social media posts mimicking news posts on Twitter, Instagram, and Facebook. Participants were asked to view examples of real and fake news taken from a news fact-checking website, Snopes, and their eye movements were recorded during the process. Participants were recruited through Prolific and SONA; the total sample size for study 1.1 was 29 participants, and the total for study 1.2 was 25 participants, after removing poor eye-tracking data. A total of five visual images comprising true and false news were shown to the participant, study 1.1 had three examples and study 1.2 had two examples. There were two main cultural backgrounds in focus: participants born in China or the United Kingdom.

Results

Results suggested that participants follow a similar visual pattern of attention to Areas of Interest (AOIs) on the posts, which leads us to believe that due to the global standardisation of popular social media platforms, a bias might have occurred during information discernment.

Discussion

It is suggested that regardless of country background, users may have similar eye-tracking results while viewing a social media post because social media platform formats are standardised globally. Further research would recommend looking at language and linguistic traits when seeking differences between country backgrounds during online information discernment.
Association between smoking and non-alcoholic fatty liver disease in Southeast Asia
Authors: Mumtaz Hassan , Hameed Madiha , Sangah Abdul Basit , Zubair Amraha , Hasan Mohammad
Published: December 2022

An estimated 8 million people die each year from tobacco smoking, with an increasing frequency recently being observed in Southeast Asian countries, which is a preventable risk factor for mortality. NAFLD, fibrosis, advancement of hepatocellular carcinoma, and prognosis for those with severe liver disease are all negatively influenced. NAFLD and cigarette usage seem to be a direct link. Oxidative stress and oncogenic signals have been implicated in cancer development in animal models and human clinical trials. The elevated risk of cardiovascular disease and malignancies in those with steatohepatitis and those who have had liver transplants is exacerbated by smoking. We found that smoking cessation may increase treatment response and fibrosis regression rates, decrease hepatocellular carcinoma incidence, and improve liver transplant outcomes. In the last segment, we'll look at electronic cigarettes, a hot subject in public health right now, as well as additional repercussions of smoking.
The combined impact of redcedar encroachment and climate change on water resources in the Nebraska Sand Hills
Authors: Kishawi Yaser , Mittelstet Aaron R. , Adane Zablon , Shrestha Nawaraj , Nasta Paolo
Published: December 2022

The Nebraska Sand Hills (NSH) is considered a major recharge zone for the High Plains Aquifer in the central United States. The uncontrolled expansion of the eastern redcedar (Juniperus Virginiana) under climate warming is posing threats to surface water and groundwater resources. The combined impact of land use and climate change on the water balance in the Upper Middle Loup River watershed (4,954 km2) in the NSH was evaluated by simulating different combinations of model scenarios using the Soil Water Assessment Tool (SWAT) model. A total of 222 climate models were ranked according to the aridity index and three models representing wet, median (most likely), and dry conditions were selected. Additionally, the impacts of carbon dioxide (CO2) emissions on root water uptake were simulated. Four plausible redcedar encroachment scenarios, namely 0.5% (no encroachment), 2.4, 4.6, and 11.9%, were considered in the numerical simulations. We, therefore, built: i) the historical scenario (2000–2019) with the current climate and redcedar cover leading to baseline results; ii) the most-likely future scenario (2020–2099) with projected climate (50th percentile of aridity index distribution) and redcedar encroachment that was estimated by using a combination of neural network and Markov-chain cellular automata model; iii) 16 future scenarios (2020–2099) with different combinations of extreme climate (5th and 95th percentiles of aridity index distribution) and four hypothetical encroachment scenarios (0.5, 2.4, 4.6, and 11.9%). The most-likely climate projection indicates that a warming pattern will be expected with a 4.1°C increase in average over the 100-year period, and this will be associated with lower-than-normal precipitation (P). Nevertheless, the concurrent increase in temperature and CO2 concentration is likely to induce stomata closure by reducing potential (ETp) and actual (ETa) evapotranspiration losses. Projected P and ETa are expected to decrease by 10 and 14% while recharge (R) and discharge (D) are expected to increase by 38 and 30% for the period 2020-2050. For the period 2051-2099, the projected P and ETa are expected to decrease by 8 and 32% while R and D are expected to increase by 140.2 and 40%. Finally, a sensitivity analysis of 16 combined climate and land use scenarios is presented and discussed. The scenario modeling approach presented in this paper can support decision-making by stakeholders for optimal management of water resources.
Effect of Cold-Plasma-Treated Phosphate Solution to Substitute Partial Nitrite on the Color, Texture, and Flavor of Smoked Sausage
Authors: Meng Dejuan , Yang Xinyu , Liu Huan , Zhang Dequan , Hou Chengli , Wang Zhenyu
Published: December 2022

There are several alternative technologies to nitrite use in meat products, including cold plasma. In this study, a cold-plasma-treated phosphate solution was added to smoked sausage, as a new ingredient. Subsequently, the color, texture, and flavor of the samples were analyzed. The results showed that, compared with nitrite (0.075 g/kg nitrite added to sausage), the addition of 30~90% nitrite and cold-plasma-treated phosphate solution had no significant effect on the a* value or the relative content of oxygenated myoglobin (p > 0.05). The amount of residual nitrite in the smoked sausage prepared with the addition of 30~70% nitrite and cold-plasma-treated phosphate solution was significantly lower than that of the nitrite-treated group. The addition of nitrite combined with cold-plasma-treated phosphate solution had no significant effects on the texture (hardness, springiness, cohesiveness, and resilience) or the sensory evaluation of the smoked sausage. A total of 69 volatile compounds were detected, and 20 of them had VIP (Variable Importance Plot) scores higher than one. In conclusion, cold plasma treatment represents a potential technology to partially substitute nitrite. This study provides new methods for the application of this nitrite substitute.
Minocycline Inhibits Microglial Activation and Improves Visual Function in a Chronic Model of Age-Related Retinal Degeneration
Authors: Du Xuan , Byrne Eimear M. , Chen Mei , Xu Heping
Published: December 2022

Age-related macular degeneration (AMD) is a chronic disease, which progresses slowly from early to late stages over many years. Inflammation critically contributes to the pathogenesis of AMD. Here, we investigated the therapeutic potential of minocycline in a chronic model of AMD (i.e., the LysMCre-Socs3fl/flCx3cr1gfp/gfp double knockout [DKO] mice). Five-month-old DKO and wild type (WT) (Socs3fl/fl) mice were gavage fed with minocycline (25 mg/kg daily) or vehicle (distilled water) for 3 months. At the end of the treatment, visual function and retinal changes were examined clinically (using electroretinography, fundus photograph and optic coherence tomography) and immunohistologically. Three months of minocycline treatment did not affect the body weight, behaviour and general health of WT and DKO mice. Minocycline treatment enhanced the a-/b-wave aptitudes and increased retinal thickness in both WT and DKO. DKO mouse retina expressed higher levels of Il1b, CD68 and CD86 and had mild microglial activation, and decreased numbers of arrestin+ photoreceptors, PKCα+ and secretagogin+ bipolar cells compared to WT mouse retina. Minocycline treatment reduced microglial activation and rescued retinal neuronal loss in DKO mice. Our results suggest that long-term minocycline treatment is safe and effective in controlling microglial activation and preserving visual function in chronic models of AMD.
Landscape of Savolitinib Development for the Treatment of Non-Small Cell Lung Cancer with MET Alteration—A Narrative Review
Authors: Zhu Xiaokuan , Lu Yao , Lu Shun
Published: December 2022

Non-small cell lung cancer (NSCLC) is increasingly being treated with targeted therapies. Savolitinib (Orpathys®) is highly selective mesenchymal epithelial transition (MET)–tyrosine kinase inhibitor (TKI), which is conditionally approved in China for advanced NSCLC with MET exon 14 skipping mutations (METex14). This article summarizes the clinical development of savolitinib, as a monotherapy in NSCLC with METex14 mutation and in combination with epidermal growth factor receptor (EGFR) inhibitor in post EGFR–TKI resistance NSCLC due to MET-based acquired resistance. Preclinical models demonstrated anti-tumor activities in MET-driven cancer cell line and xenograft tumor models. The Phase Ia/Ib study established an optimized, recommended phase II dose in Chinese NSCLC patients, while TATTON study of savolitinib plus osimertinib in patients with EGFR mutant, MET-amplified and TKI-progressed NSCLC showed beneficial efficacy with acceptable safety profile. In a pivotal phase II study, Chinese patients with pulmonary sarcomatoid carcinoma, brain metastasis and other NSCLC subtype positive for METex14 mutation showed notable responses and acceptable safety profile with savolitinib. Currently, results from ongoing clinical trials are eagerly anticipated to confirm the efficacious and safety benefits of savolitinib as monotherapy and in combination with EGFR–TKI in acquired resistance setting in advanced NSCLC and its subtypes with MET alterations.
Coupling Relationship between Ecosystem Service Value and Socioeconomic Development in the Qinba Mountains, China
Authors: Ma Qi , Zhou Mengquan , Liu Jiechao , Zhao Jing , Xi Meijun
Published: December 2022

The degree of coordination between ecosystem services and the level of socioeconomic development has essential implications for regional sustainability. The coordinated development of ecology and economy is a major theoretical and practical problem for the Qinba Mountains, which is once one of the 14 contiguous destitute areas in China. Based on the land use and cover change (LUCC) data interpreted by medium-resolution remote sensing images, this study adopted the modified equivalent factor method to calculate ecosystem service value (ESV) and its temporal and spatial variation in the Qinba Mountains. A comprehensive index system was constructed to measure the socioeconomic development level and to reveal the coupling relationship between the ESV and socioeconomic development. The results show that: (1) for 2000–2015, the ESV in these areas was increasing, the proportion of forest ESV was the largest, and it increased significantly. (2) The level of socioeconomic development was constantly improving, and the differences within the region were gradually emerging. (3) Except for the Hantai District, which has been in a highly uncoordinated state, the degree of coordination between ESV and socioeconomic development has improved year by year, and most counties were in a state of medium coordination, or above. The results contribute to a scientific basis for decision making regarding ecological environmental protection and green economic development in the Qinba Mountains, and have positive significance for promoting the construction of ecological civilization and sustainable development in the study area.
Transfer of the Resistance to Multiple Diseases from a Triticum-Secale-Thinopyrum Trigeneric Hybrid to Ningmai 13 and Yangmai 23 Wheat Using Specific Molecular Markers and GISH
Authors: Dai Yi , Shi Juntao , Li Jinfeng , Gao Yujiao , Ma Haigang , Wang Yonggang , Wang Baotong , Chen Jianmin , Cheng Peng , Ma Hongxiang
Published: December 2022

The middle to lower reaches of the Yangtze River are China’s second largest area for wheat production; wheat disease is more serious there than in other areas because of the high humidity and warm weather. However, most cultivated varieties are susceptible to Fusarium head blight (FHB), powdery mildew, and stripe rust, and the lack of disease-resistant germplasm is an obstacle in wheat breeding. Rye and Thinopyrum elongatum, related species of wheat, carry many genes involved in disease resistance. In this study, a trigeneric hybrid, YZU21, with resistance to FHB, powdery mildew, and stripe rust was used to improve two major wheat cultivars, Ningmai 13 (NM13) and Yangmai 23 (YM23). Specific molecular markers and GISH were used to identify hybrid progenies. Five addition or substitution lines and one translocation line of the Triticum-Secale-Thinopyrum trigeneric hybrid were obtained and evaluated for agronomic traits and the resistance to multiple diseases. The results showed that the six trigeneric hybrid lines had desirable agronomic traits and improved resistance to FHB, powdery mildew, and stripe rust; they might be used as parents in wheat breeding for the resistance to multiple disease.
The Theory of Carcino-Evo-Devo and Its Non-Trivial Predictions
Authors: Kozlov A. P.
Published: December 2022

To explain the sources of additional cell masses in the evolution of multicellular organisms, the theory of carcino-evo-devo, or evolution by tumor neofunctionalization, has been developed. The important demand for a new theory in experimental science is the capability to formulate non-trivial predictions which can be experimentally confirmed. Several non-trivial predictions were formulated using carcino-evo-devo theory, four of which are discussed in the present paper: (1) The number of cellular oncogenes should correspond to the number of cell types in the organism. The evolution of oncogenes, tumor suppressor and differentiation gene classes should proceed concurrently. (2) Evolutionarily new and evolving genes should be specifically expressed in tumors (TSEEN genes). (3) Human orthologs of fish TSEEN genes should acquire progressive functions connected with new cell types, tissues and organs. (4) Selection of tumors for new functions in the organism is possible. Evolutionarily novel organs should recapitulate tumor features in their development. As shown in this paper, these predictions have been confirmed by the laboratory of the author. Thus, we have shown that carcino-evo-devo theory has predictive power, fulfilling a fundamental requirement for a new theory.