bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of Asphalt Behavior on Pre-Wet Aggregate Surface Based on Molecular Dynamics Simulation and Surface Energy Theory
Authors: Cao Yaoxi , Wang Yanhua , Li He , Chen Wuxing
Published: October 2023

The improvement of the performance of asphalt mixtures has been studied by scholars. This research proposes a new asphalt–mineral interface formation method, which is a pre-wet bitumen–mineral mixture. The formation process of the pre-wet asphalt–mineral interface was simulated by molecular dynamics simulation software. The diffusion coefficient, concentration distribution, and interfacial energy of the asphalt on the surface of the pre-wet mineral material and non-pre-wet mineral material were compared and analyzed. The simulation results show that the mineral surface diffusion rate of the asphalt after pre-wetting is increased by more than 50%, and the concentration in the X, Y, and Z directions is reduced by 0.8%, 4.6%, and 7.8%, respectively. At the same time, the interface energy between the bitumen and the pre-wet mineral was increased by more than 8%. The results of the molecular dynamics model are verified based on the surface energy theory and contact angle test. The experimental results show that the contact angle of the asphalt is smaller and the diffusion performance is better after pre-wetting. At the same time, the interface adhesion work between the asphalt and wet mineral surface increased by 4.3% in a dry environment, and the peeling work decreased by 41.1% in a water environment. Therefore, the author believes that the pre-wetting technology of the asphalt mixture has a certain feasibility and practicability.
Crosstalk Reduction in High-Density Radio Frequency Printed Circuit Boards: Leveraging FR4 Coating Layers
Authors: Barzdenas Vaidotas , Vasjanov Aleksandr
Published: October 2023

The escalating component density in radio frequency (RF) systems presents a growing challenge related to the coupling of adjacent microstrip lines in high-density printed circuit boards (PCBs). As a result, to tackle this prominent issue, there is a continuous pursuit of innovative techniques to effectively minimize the coupling effects among closely spaced microstrip lines. This paper proposes a reduction in the coupling of adjacent lines by utilizing a coating (stiffener) layer, which is commonly used in rigid-flex PCB fabrication. For this purpose, a reference 50 Ohm coupled line performance was compared to three coupled lines with track widths of 1.39 mm, 1.30 mm, and 1.25 mm, respectively, all at a fixed distance between the tracks. These decreasing widths were used to achieve the same 50 Ohm impedance for the coupled lines when covered with different coating layers. Each of these three coupled lines was covered with different coating (stiffener) layers, measuring 0.1 mm, 0.3 mm, and 0.5 mm in thickness, respectively. The manufactured device under test (DUT) structures underwent time-domain reflectometry (TDR) and S-parameter measurements. The TDR measurements of the DUT structures with coating layers demonstrated excellent conformity to the 50 Ohm reference coupled line. Meanwhile, the S21 measurements indicated a significant decrease in the crosstalk. For example, for a coating layer thickness of 0.3 mm, the crosstalk decreased by approximately 5–6 dB within the frequency range up to 5 GHz. When the coating layer thickness was 0.5 mm, the crosstalk decreased by approximately 10 dB or more.
Suppression of Secondary Electron Emissions on the Graphene-Coated Polyimide Materials Prepared by Chemical Vapor Deposition
Authors: Qi Xin , Ma Yanzhao , Liu Sisheng , Nie Xiangyu , Zhang Tao , Wu Yong , Peng Weiping , Hu Guoming
Published: October 2023

Polyimide thin-film materials are widely used in aerospace and particle gas pedals, etc., but the phenomenon of secondary electron emission occurred under vacuum conditions. The graphene-coated polyimide materials were prepared for this phenomenon to suppress secondary electron emissions. The graphene coating was prepared on the polyimide surface through chemical vapor deposition (CVD). Scanning electron microscope images (SEM), X-ray photoelectron spectrometer images (XPS), Raman spectroscopy, atomic force microscopy (AFM), and other analytical methods were used to characterize the properties of the prepared materials. The C1s XPS fine spectra and Raman curve analyses showed that the material has an abundant sp2 hybridized structure, and the sp2 structure can reduce secondary electron emissions. The C, O, and N contents in the tested samples were 65.85, 20.47, and 13.68 at.%, respectively. It was examined that the graphene coating had an inhibitory effect on the secondary electron emissions of polyimide materials, and the secondary electron emission yield (SEY) was significantly reduced. The results of secondary electron tests showed that the maximum SEY (δmax) of the polyimide material decreased from 1.72 to 1.52 after the preparation of the graphene coating. The mechanism of using a graphene coating to reduce the SEY of polyimide was analyzed from experimental and theoretical perspectives. The results of this study can provide research ideas for polyimide thin film materials in aerospace, particle gas pedals, and other applications.
Engineering Process Optimization and Quality Stability Control of High-Speed Laser Cladding Coatings Based on AHP-FCE
Authors: Xv Yifei , Sun Yaoning , Cheng Wangjun , Zhang Yuhang
Published: October 2023

Due to the rapid advancement in processing efficiency, high-speed laser cladding has demonstrated significant potential in the repair and protection of various substrates. In this study, we established a comprehensive evaluation model for the coating quality of Fe-Cr-Ni-based alloy with high-speed laser cladding using the analytic hierarchy process and fuzzy comprehensive evaluation method (AHP-FCE). The weights obtained through the analytic hierarchy process for forming quality, microstructure, and surface performance are as follows: WB1 = 0.1365, WB2 = 0.2385, and WB3 = 0.625, respectively. During the fuzzy comprehensive evaluation step, an evaluation level was graded while quantifying the level range through membership function judgment. By combining subjective and objective evaluations, qualitative issues were transformed into quantitative assessment methods. Through comprehensive evaluation analysis, it was concluded that the scanning speed of high-speed laser cladding had a greater impact on coating thickness compared to powder feeding speed while significantly enhancing microstructure densification. The overlap rate exerted the most influence on dilution rate homogenization of near-surface dendrites. Simultaneously, the optimal preparation technology was determined: laser power 660 W, scanning speed 14,400 mm/min, overlap rate/min. This study transforms multi-objective quality evaluation of high-speed laser cladding coatings into a single objective problem by realizing comprehensive quality quantification and providing a new method for quantitative evaluation and visualization of coating quality.
Classifying the Main Technology Clusters and Assignees of Home Automation Networks Using Patent Classifications
Authors: Charmanas Konstantinos , Georgiou Konstantinos , Mittas Nikolaos , Angelis Lefteris
Published: October 2023

Home automation technologies are a vital part of humanity, as they provide convenience in otherwise mundane and repetitive tasks. In recent years, given the development of the Internet of Things (IoT) and artificial intelligence (AI) sectors, these technologies have seen a tremendous rise, both in the methodologies utilized and in their industrial impact. Hence, many organizations and companies are securing commercial rights by patenting such technologies. In this study, we employ an analysis of 8482 home automation patents from the United States Patent and Trademark Office (USPTO) to extract thematic clusters and distinguish those that drive the market and those that have declined over the course of time. Moreover, we identify prevalent competitors per cluster and analyze the results under the spectrum of their market impact and objectives. The key findings indicate that home automation networks encompass a variety of technological areas and organizations with diverse interests.
Structural, Electrical, and Electrochemical Properties of a Na2O-V2O5 Ceramic Nanocomposite as an Active Cathode Material for a Na-Ion Battery
Authors: Ibrahim Ahmed , Watanabe Satoshi , Razum Marta , Pavić Luka , Homonnay Zoltán , Kuzmann Ernő , Hassaan Mohamed Yousry , Kubuki Shiro
Published: October 2023

In this paper, a relationship between the structure and the electrical properties of a nanocrystalline composite ceramics xNa2O·(100 − x)V2O5 with ‘x’ of 5, 15, 25, 35, and 45 mol%, abbreviated as xNV, was investigated by X-ray diffractometry (XRD), X-ray absorption spectroscopy (XAS), Cyclic Voltammetry (CV), Electrochemical impedance spectroscopy (EIS), and cathode active performance in Na-ion battery (SIB). For the expected sodium vanadium bronzes (NaxV2O5) precipitation, the preparation of xNV was performed by keeping the system in the molten state at 1200 °C for one hour, followed by a temperature decrease in the electric furnace to room temperature at a cooling rate of 10 °C min−1. XRD patterns of the 15NV ceramic exhibited the formation of Na0.33V2O5 and NaV3O8 crystalline phases. Moreover, the V K-edge XANES showed that the absorption edge energy of ceramics 15NV recorded at 5479 eV is smaller than that of V2O5 at 5481 eV, evidently indicating a partial reduction from V5+ to V4+ due to the precipitation of Na0.33V2O5. In the cyclic voltammetry, reduction peaks of 15NV were observed at 1.12, 1.78 V, and 2.69 V, while the oxidation peak showed up only at 2.36 V. The values of the reduction peaks were related to the NaV3O8 crystalline phase. Moreover, the diffusion coefficient of Na+ (DNa+) gradually decreased from 8.28 × 10−11 cm2 s−1 to 1.23 × 10−12 cm2 s−1 with increasing Na2O content (x) from 5 to 45 mol%. In the evaluation of the active cathode performance of xNV in SIB, ceramics 15NV showed the highest discharge capacity 203 mAh g−1 at a current rate of 50 mA g−1. In the wider voltage range from 0.8 to 3.6 V, the capacity retention was maintained at 50% after 30 cycles, while it was significantly improved to 90% in the narrower voltage range from 1.8 to 4.0 V, although the initial capacity decreased to 56 mAh g−1. It is concluded that the precipitation of the Na0.33V2O5 phase improved the structural and electrical properties of 15NV, which provides a high capacity for the Na-ion battery when incorporated as a cathode active material.
Research on the Reinforcement and Inhibition of Water–Salt Activity in Mural Ground Layers by Superhydrophobic SiO2 Particles
Authors: Xia Qixing , Dong Wenqiang
Published: October 2023

Due to notable water–salt activities, salt damage easily recurs and becomes one of the biggest challenges for the protection of ancient murals. Herein, superhydrophobic SiO2 materials with different sizes were used to modify mural ground layer substrates, and the improvement effect mechanisms were systematically evaluated with scanning electron microscopy (SEM), X-ray diffraction (XRD), laser scanning confocal microscopy (LSCM), and a contact angle instrument. The results show that the superhydrophobic SiO2 can spread into the substrates though holes and cracks and further increase the contact angles of the substrates to water droplets. Compared with the initial ground layer substrate, the substrates treated with the superhydrophobic SiO2 possess stronger mechanical strength and a better ability in suppressing water–salt activity. In particular, larger-size SiO2 (mSiO2) maintains better mechanical reinforcement in the substrates, because mSiO2 can provide better support in the internal gaps of the substrates. By contrast, nSiO2 can spread deeper into the substrate than mSiO2, and more greatly improve the contact angle to water droplets, endowing nSiO2 with a better ability to restrain water–salt activity. Our study provides an alternative idea for solving salt damage in murals, and promotes the application of SiO2 materials in heritage conservation.
Simulation and Experimental Study of Hot Deformation Behavior in Near β Phase Region for TC21 Alloy with a Forged Structure
Authors: Ji Xuanming , Tian Qimei , Tan Yuanbiao , Huang Chaowen , Wan Mingpan , Li Rudong
Published: October 2023

Quasi-beta processing was considered to be a promising processing method to obtain a component with excellent mechanical properties. To achieve an optimized quasi-beta processing parameter for TC21 alloys, the hot deformation behavior in the near β phase region for the alloy with a forged structure was investigated by the thermal compression test and finite element (FEM) simulation. The obtained results indicated that the flow behavior of the samples was significantly influenced by the hot deformation parameters, and it exhibited a flow hardening behavior at the start stage of deformation. Based on the experimental data, the constitutive equation and processing maps were obtained. The optimum hot processing parameter was 986 °C/10−3 s−1. Based on the FEM simulation results, the evolution of the temperature field, strain field, and stress field in the deformed samples at different strains exhibited a similar trend in the unstable region, which was distributed symmetrically along the center line of the samples, with the center area of the samples being the highest and the center area of the section being the lowest.
The Value of Micro-CT in the Diagnosis of Lung Carcinoma: A Radio-Histopathological Perspective
Authors: Dizbay Sak Serpil , Sevim Selim , Buyuksungur Arda , Kayı Cangır Ayten , Orhan Kaan
Published: October 2023

Micro-computed tomography (micro-CT) is a relatively new imaging modality and the three-dimensional (3D) images obtained via micro-CT allow researchers to collect both quantitative and qualitative information on various types of samples. Micro-CT could potentially be used to examine human diseases and several studies have been published on this topic in the last decade. In this study, the potential uses of micro-CT in understanding and evaluating lung carcinoma and the relevant studies conducted on lung and other tumors are summarized. Currently, the resolution of benchtop laboratory micro-CT units has not reached the levels that can be obtained with light microscopy, and it is not possible to detect the histopathological features (e.g., tumor type, adenocarcinoma pattern, spread through air spaces) required for lung cancer management. However, its ability to provide 3D images in any plane of section, without disturbing the integrity of the specimen, suggests that it can be used as an auxiliary technique, especially in surgical margin examination, the evaluation of tumor invasion in the entire specimen, and calculation of primary and metastatic tumor volume. Along with future developments in micro-CT technology, it can be expected that the image resolution will gradually improve, the examination time will decrease, and the relevant software will be more user friendly. As a result of these developments, micro-CT may enter pathology laboratories as an auxiliary method in the pathological evaluation of lung tumors. However, the safety, performance, and cost effectiveness of micro-CT in the areas of possible clinical application should be investigated. If micro-CT passes all these tests, it may lead to the convergence of radiology and pathology applications performed independently in separate units today, and the birth of a new type of diagnostician who has equal knowledge of the histological and radiological features of tumors.
Professional Learning Communities in Chinese Preschools: Challenging Western Frameworks
Authors: Bautista Alfredo , Xu Rongrong , Cen Fangmei , Yang Weipeng
Published: October 2023

In the education field, professional learning communities (PLCs) are regarded as an effective method for fostering professional development for teachers, enhancing student learning and achievement, and promoting school reform. Quality leadership and management are understood to be essential for schools to become effective PLCs. Mainly proposed by Western scholars, existing PLC frameworks explicitly or implicitly assume that PLCs should be initiated and led by teachers themselves, following a bottom-up approach. The goal of this conceptual overview paper is to challenge certain taken-for-granted core principles of Western PLC frameworks by analyzing the current approach to PLCs adopted by preschools in mainland China, which follow a top-down leadership and management approach. We review multiple research streams, including policy frameworks and regulations, as well as the most relevant conceptual, theoretical, and empirical studies published in recent years. After reviewing China’s core socio-cultural traditional values, we describe the recent policies that have mandated the establishment and implementation of PLCs in Chinese preschools. We then review several recent studies that demonstrate that, despite profound differences to Western frameworks, PLCs in Chinese preschools are effective in benefitting teachers and highly regarded by the relevant stakeholders. The literature shows that Chinese preschools enact culturally situated versions of PLCs, characterized by some standard PLC principles (e.g., shared vision, collaboration, and collective responsibility) alongside features that resonate with Confucian values (e.g., strong hierarchical structure, guidance of external experts, internal leadership support, and greater emphasis on ecological support rather than personal agency and autonomy). We conclude that Chinese preschools merit international attention, as their approach to leadership and management allow us to reconsider certain core principles of Western PLC frameworks. Future directions for PLC policy and practice are suggested.
Refined Analysis of Leakage Current in SiC Power Metal Oxide Semiconductor Field Effect Transistors after Heavy Ion Irradiation
Authors: Xiang Yutang , Liang Xiaowen , Feng Jie , Feng Haonan , Zhang Dan , Wei Ying , Yu Xuefeng , Guo Qi
Published: October 2023

A leakage current is the most critical parameter to characterize heavy ion radiation damage in SiC MOSFETs. An accurate and refined analysis of the source and generation process of a leakage current is the key to revealing the failure mechanism. Therefore, this article finely tests the online and post-irradiation leakage changes and leakage pathways of SiC MOSFETs caused by heavy ion irradiation, analyzes the damaged location of the device in reverse, and discusses the mechanism of leakage generation. The experimental results further confirm that an increase in the leakage current of a device during heavy ion irradiation is positively correlated with the applied voltage of the drain, but the leakage path is not direct from the drain to the source. The experimental analysis of the source of the leakage current of the device after irradiation indicates that there is also a leakage current path between the device gate and source. The research results suggest that the experimental sample is more prone to a single-event gate rupture effect under this heavy ion radiation condition. The gate breakdown mainly occurs in the gate oxide layer at the neck region. This research can provide a theoretical basis for the radiation resistance reinforcement of SiC power devices.
Pattern Orientation Finder (POF): A Robust, Bio-Inspired Light Algorithm for Pattern Orientation Measurement
Authors: Carlini Alessandro , Paindavoine Michel
Published: October 2023

We present the Pattern Orientation Finder (POF), an innovative, bio-inspired algorithm for measuring the orientation of patterns of parallel elements. The POF was developed to obtain an autonomous navigation system for drones inspecting vegetable cultivations. The main challenge was to obtain an accurate and reliable measurement of orientation despite the high level of noise that characterizes aerial views of vegetable crops. The POF algorithm is computationally light and operable on embedded systems. We assessed the performance of the POF algorithm using images of different cultivation types. The outcomes were examined in light of the accuracy and reliability of the measurement; special attention was paid to the relationship between performance and parameterization. The results show that the POF guarantees excellent performance, even in more challenging conditions. The POF shows high reliability and robustness, even in high-noise contexts. Finally, tests on images from different sectors suggest that the POF has excellent potential for application to other fields as well.
Profitability of Hydrogen-Based Microgrids: A Novel Economic Analysis in Terms of Electricity Price and Equipment Costs
Authors: Rey Jesús , Segura Francisca , Andújar José Manuel
Published: October 2023

The current need to reduce carbon emissions makes hydrogen use essential for self-consumption in microgrids. To make a profitability analysis of a microgrid, the influence of equipment costs and the electricity price must be known. This paper studies the cost-effective electricity price (EUR/kWh) for a microgrid located at ‘’La Rábida Campus’’ (University of Huelva, south of Spain), for two different energy-management systems (EMSs): hydrogen-priority strategy and battery-priority strategy. The profitability analysis is based, on one hand, on the hydrogen-systems’ cost reduction (%) and, on the other hand, considering renewable energy sources (RESs) and energy storage systems (ESSs), on cost reduction (%). Due to technological advances, microgrid-element costs are expected to decrease over time; therefore, future profitable electricity prices will be even lower. Results show a cost-effective electricity price ranging from 0.61 EUR/kWh to 0.16 EUR/kWh for hydrogen-priority EMSs and from 0.4 EUR/kWh to 0.17 EUR/kWh for battery-priority EMSs (0 and 100% hydrogen-system cost reduction, respectively). These figures still decrease sharply if RES and ESS cost reductions are considered. In the current scenario of uncertainty in electricity prices, the microgrid studied may become economically competitive in the near future.
A Survey of Ultra-Low-Power Amplifiers for Internet of Things Nodes
Authors: Grasso Alfio Dario , Pennisi Salvatore , Venezia Chiara
Published: October 2023

This paper investigates CMOS operational transconductance amplifier (OTA) design methodologies suitable for Internet of Things nodes. The use of MOS transistors in the subthreshold of the body terminal for signal input or bias, as well as newer inverter- and digital-based techniques, is considered. Solutions from the authors’ work are utilized as main case examples. State-of-the-art ultra-low-power OTAs are then thoroughly compared using a data-driven approach. According to the findings, digital- and inverter-based solutions have the lowest area occupation and superior small-signal performance but are inherently susceptible to process, supply, and temperature (PVT) variations. The only “analog” approach suitable for a sub-0.6 V supply is body driving in conjunction with subthreshold bias. It offers competitive large-signal performance and, more importantly, is less sensitive to PVT variations at the expense of silicon area.
Home Trash Biomass Valorization by Catalytic Pyrolysis
Authors: Rijo Bruna , Soares Dias Ana Paula , de Jesus Nicole , Pereira Manuel Francisco
Published: October 2023

With the increase in population, large amounts of food waste are produced worldwide every day. These leftovers can be used as a source of lignocellulosic waste, oils, and polysaccharides for renewable fuels. In a fixed bed reactor, low-temperature catalytic pyrolysis was investigated using biomass gathered from domestic garbage. Thermogravimetry, under N2 flow, was used to assess the pyrolysis behavior of tea and coffee grounds, white potato, sweet potato, banana peels, walnut, almonds, and hazelnut shells. A mixture of biomass was also evaluated by thermogravimetry. Waste inorganic materials (marble, limestone, dolomite, bauxite, and spent Fluid Catalytic Cracking (FCC) catalyst) were used as catalysts (16.7% wt.) in the pyrolysis studies at 400 °C in a fixed bed reactor. Yields of bio-oil in the 22–36% wt. range were attained. All of the catalysts promoted gasification and a decrease in the bio-oil carboxylic acids content. The marble dust catalyst increased the bio-oil volatility. The results show that it is possible to valorize lignocellulosic household waste by pyrolysis using inorganic waste materials as catalysts.
Effects of Processing Technology on Protein Separation, Quality, and Functional Characteristics
Authors: Wang Zhongjiang , Sun Fuwei
Published: October 2023

Proteins provide the material foundation of all life activities and play an important role in the physiological and biochemical metabolism of the human body [...]
Effects of Casein Hydrolysate Prepared with Savinase on the Quality of Bread Made by Frozen Dough
Authors: Bekiroglu Hatice , Ozulku Gorkem , Sagdic Osman
Published: October 2023

The effect of casein savinase hydrolysate (CSH) usage on frozen dough (1%, 1.5% and 2%, g/100 g flour) was investigated in terms of rheological, thermal and structural characteristics of wheat doughs and the textural and color properties of corresponding breads. Rheological measurements showed that CSH addition into dough led to a reduction in G′ and G″ values, but a similar trend was not observed in frozen dough samples. The increase in protein band intensity was observed for control dough (CD) after frozen storage (−30 °C, 28 days), while there were no increases in the band intensities of the doughs with CSH. The freezable water content of unfrozen doughs decreased gradually with the addition of CSH, dependent on concentration level. Frozen storage caused a notable reduction in the α-helices structure of the CD sample (p < 0.05) while no significant variation was observed for the doughs containing CSH (p > 0.05). The lowest specific volume reduction and hardness increment were observed for the breads containing 1.5% and 2% CSH. Frozen storage caused a significant reduction in the b* value of bread crust (p < 0.05), while no significant effect was observed for L* and a* value during frozen storage (p > 0.05). Overall, CSH incorporation into frozen dough can be an alternative that could reduce the quality deterioration of frozen bread.
Relationship between the Dynamics of Flavor Compounds and Microbial Succession in the Natural Fermentation of Zhalajiao, a Popular Traditional Chinese Fermented Chili Paste
Authors: Huang Luhan , Tang Yanyan , Zheng Jiong , Kan Jianquan , Wu Yun , Wu Yating , Awad Sameh , Ibrahim Amel , Du Muying
Published: October 2023

Zhalajiao, a traditional Chinese fermented food, is popular due to its unique flavor. Traditional Zhalajiao fermentation is closely related to flavor compounds production. However, the mechanisms underlying the formation of these crucial flavor components in Zhalajiao remain unclear. Here, we explored the dynamic changes in physical and chemical properties, microbial diversity, and flavor components of Zhalajiao at various fermentation times. In total, 6 organic acids, 17 amino acids, and 21 key volatile compounds were determined as flavor components. In Zhalajiao, Lactobacillus and Cyanobacterium were the main bacteria that were involved in the formation of crucial flavor compounds. Candida showed a significant correlation with 14 key flavor compounds during fermentation (p < 0.05) and was the main fungal genus associated with flavor formation in Zhalajiao. This research offers a theoretical foundation for the flavor regulation and quality assurance of Zhalajiao.
Formulation of Transliposomal Nanocarrier Gel Containing Strychnine for the Effective Management of Skin Cancer
Authors: Alam Perwez , Imran Mohd , Gupta Dipak Kumar , Akhtar Ali
Published: October 2023

Strychnine (STCN) has demonstrated an exceptional anticancer effect against various cancers. However, the STCN clinical utility has been hampered by its low water solubility, restricted therapeutic window, short half-life, and significant toxicity. The objective of this investigation was to design and optimize a formulation of strychnine-loaded transliposomes (STCN–TLs) for dermal administration of STCN to treat skin cancer. The formulations of STCN–TL were examined in terms of vesicle size (VS), polydispersity index (PDI), entrapment efficiency (EE), and in vitro delivery. The improved STCN–TL formulation exhibited VS, PDI, EE, and in vitro delivery of 101.5 ± 2.14 nm, 0.218 ± 0.12, 81.74 ± 1.43%, and 85.39 ± 2.33%, respectively. In an ex vivo penetration, the created STCN–TL formulation demonstrated a 2.5-fold increase in permeability compared to the STCN solution. CLSM pictures of skin (rat) revealed that the rhodamine B-loaded transliposome preparation penetrated deeper than the rhodamine B hydroalcoholic mixture. Additionally, rat skin managed with STCN–TL nanogel exhibited a significant increase in Cskin max and AUC0-8 compared to rat skin treated with traditional STCN gel. The findings demonstrated that the transliposome preparation might be a suitable nanocarrier for the cutaneous distribution of STCN in the amelioration of skin cancer.
Cryosectioning of Hydrogels as a Reliable Approach to Increase Yield and Further Tune Mechanical Properties
Authors: Martínez-Blanco África , Noé Sergio , Carreras-Vidal Lourdes , Otero Jorge , Gavara Núria
Published: October 2023

Decellularized extracellular matrix (dECM) hydrogels have emerged as promising materials in tissue engineering. The steps to produce dECM hydrogels containing the bioactive epitopes found in the native matrix are often laborious, including the initial harvesting and decellularization of the animal organ. Furthermore, resulting hydrogels often exhibit weak mechanical properties that require the use of additional crosslinkers such as genipin to truly simulate the mechanical properties of the desired study tissue. In this work, we have developed a protocol to readily obtain tens of thin dECM hydrogel cryosections attached to a glass slide as support, to serve as scaffolds for two-dimensional (2D) or three-dimensional (3D) cell culture. Following extensive atomic force microscopy (AFM)-based mechanical characterization of dECM hydrogels crosslinked with increasing genipin concentrations (5 mM, 10 mM, and 20 mM), we provide detailed protocol recommendations for achieving dECM hydrogels of any biologically relevant stiffness. Given that our protocol requires hydrogel freezing, we also confirm that the approach taken can be further used to increase the mechanical properties of the scaffold in a controlled manner exhibiting twice the stiffness in highly crosslinked arrays. Finally, we explored the effect of ethanol-based short- and long-term sterilization on dECM hydrogels, showing that in some situations it may give rise to significant changes in hydrogel mechanical properties that need to be taken into account in experimental design. The hydrogel cryosections produced were shown to be biocompatible and support cell attachment and spreading for at least 72 h in culture. In brief, our proposed method may provide several advantages for tissue engineering: (1) easy availability and reduction in preparation time, (2) increase in the total hydrogel volume eventually used for experiments being able to obtain 15–22 slides from a 250 µL hydrogel) with a (3) reduction in scaffold variability (only a 17.5 ± 9.5% intraslide variability provided by the method), and (4) compatibility with live-cell imaging techniques or further cell characterization of cells.
Basic Health Service Delivery to Vulnerable Populations in Post-Conflict Eastern Congo: Asset Mapping
Authors: Bwirire Dieudonne , Crutzen Rik , Letschert Rianne , Namegabe Edmond Ntabe , de Vries Nanne
Published: October 2023

Populations in post-conflict settings often have increased healthcare needs, residing in settings where basic services needed to maintain good health may be non-existent or hard to access. Therefore, there is a need for better identification and reallocation of resources as part of the post-conflict health rehabilitation effort. Assets can be described as the collective resources that individuals and communities have at their disposal, which protect against adverse health outcomes and promote health status. This study applies an asset-based approach to explore the most optimal design of health services and to identify the resource constraints for basic health service delivery to the most vulnerable communities in eastern Congo. We implemented the asset mapping in two phases. Firstly, we combined a qualitative survey with community walks to identify the assets already present in the communities. Secondly, we conducted group discussions to map out assets that are the core of asset-based community development (ABCD) practice. We finally documented all assets in a Community Asset Spreadsheet. Overall, 210 assets were identified as available and potentially valuable resources for the communities in eastern Congo. Among them, 57 were related to local associations, 23 to land and physical environments, 43 to local institutions, 46 to individuals, 35 to economy and exchange, and only 6 to culture, history, and stories. Drawing upon the findings of the qualitative survey, community walks, and group discussions, we concluded that an important number of resources are in place for basic health service delivery. By activating existing and potential resources, the most vulnerable populations in eastern Congo might have the required resources for basic health service delivery. Our findings support the use of an asset-mapping research method as appropriate for identifying existing and potential resources for basic health services in a post-conflict setting.
Effect of Pre-Germination Temperature Regime on Pollen Germination and Fruit Set in Pear, Pyrus bretschneiderilia
Authors: Liu Limin , Liu Ziyan , Han Hu , Qiao Baiyu , Li Yangfan , He Xiongkui , Liu Yajia
Published: October 2023

Extensive research has been conducted on the effects of pollen activation temperature, cultivation temperature, and ambient temperature on germination rates and fruit sets. Yet, the influence of the temperature (pre-germination temperature) at which the pollen suspension is prepared within the storage container (tank) remains unexplored. In this study, we initially established the optimal time for pollen activation. Subsequently, pollen suspensions at varying temperatures were prepared, and their germination rates were analyzed using microscopy. Pollen suspensions of different temperatures were then sprayed onto Fojianxi pear flowers, and parameters such as flower fruit set rate, inflorescence fruit set rate, fruit longitudinal dimension, fruit transverse dimension, and fruit shape index were evaluated. The findings revealed that the optimal pollen activation duration was 6 h. A pollen suspension temperature of 30 °C resulted in the highest germination rate (72.06 ± 6.87%). However, a suspension temperature of 25 °C achieved the highest flower fruit set rate (57.29 ± 5.58%) and inflorescence fruit set rate (87.50 ± 4.84%). The fruit longitudinal dimension (68.12 ± 3.94 mm), transverse dimension (73.77 ± 4.04 mm), and fruit shape index (108.42 ± 6.02%) were most favorable at a suspension temperature of 15 °C. Our study concludes that the pollen in lower pollen suspension temperatures (15~25 °C) has higher germination energy and have higher stigmatic capacity. Thus, we advocate for a pollen suspension temperature range of 15~25 °C when employing liquid spray pollination techniques with Xuehuali (Pyrus bretschneiderilia) pollen.
Prevalence of Impaired Physical Mobility in Dialysis Patients: A Single-Centre Cross-Sectional Study
Authors: Bogataj Špela , Pajek Jernej , Slonjšak Blaž , Peršič Vanja
Published: October 2023

Impaired physical mobility in hemodialysis (HD) patients is considered an important modifiable risk factor of increased all-cause morbidity and mortality. To our knowledge, no study to date has determined the overall burden of limited physical mobility in prevalent HD patients. The aim of this research is to investigate impaired physical mobility and its clinical correlates. We conducted a cross-sectional observational study in all patients of the Centre for Acute and Complicated Dialysis at the University Medical Centre of Ljubljana, where the most complex patients receive HD on average three times per week. The data were collected through interviews based on a prepared questionnaire and medical history review. A total of 205 patients were included in this study (63.9 ± 15.4 years). Sixty percent (122/205) of the patients had little or no physical mobility impairment, and others were categorized with a minor or severe mobility limitation. A minor mobility impairment was found in 21% (43/205) of patients: 10 patients (5%) used a mobility aid in the form of a crutch, 9 patients (4%) were dependent on two crutches or a walker, and 24 patients (12%) were temporarily dependent on the assistance of a third person. Severe mobility limitations were observed in 22% (40/205) of patients, ranging from being confined to bed (19/205, 9%), confined to bed but able to perform some movements (19/205, 9%), and ambulatory but dependent on the assistance of a third person for locomotion (2/205, 1%). The most common causes of the limitation of mobility were neurological (19/40, 47.5%), cardiovascular (9/40, 22.5%), musculoskeletal (8/40, 20%), and other causes (4/40, 10%). A significant, moderate positive correlation was observed between mobility problems and the age of the participants (r = 0.36, p < 0.001), while a significant, small positive correlation was obtained between the mobility problems and C-reactive protein (r = 0.15, p = 0.044). Moreover, mobility problems had a small but significant negative correlation with albumin levels (r = −0.15, p = 0.042). When controlling for age, results yield no significant correlations, and, in regression analysis, only the age (p < 0.001) and male gender (p = 0.007) of the participants were independent predictors of mobility impairment. We conclude that impaired mobility has a high overall prevalence among chronic HD patients. Strategies to prevent and improve mobility limitations are strongly needed.
Active Neurodynamics at Home in Patients with Knee Osteoarthritis: A Feasibility Study
Authors: Serrano-García Beatriz , Forriol-Campos Francisco , Zuil-Escobar Juan Carlos
Published: October 2023

The aim was to evaluate the feasibility of a home-based neurodynamic programme for patients with knee osteoarthritis (KO). Thirty participants (70% women) ≥ 50 years old with KO (Kellgren–Lawrence grades I–II) were included. Active mobilisation of the femoral nerve was performed at home over a period of 6–8 weeks. The feasibility of the programme was assessed using a survey that included questions related to understanding of the activity; adherence to the intervention; the burden caused by the intervention; self-perceived effects on the participant; follow-up; the barriers; and facilitators. Pain intensity, using the numerical rating scale (NRS); pressure pain thresholds (PPT); temporal assessment; pain modulation; Knee Injury and Osteoarthritis Outcome Score (KOOS), 12-item Short Form Survey questionnaire (SF-12), and the Central Sensitization Inventory questionnaire (CSI) were also collected, before and after the intervention. All patients performed the intervention, completed at least 42 days of activity, and considered the exercise adequate, with 28 participants (93.3%) reporting that the intervention was good for them. Statistically significant values (p < 0.05) were found for NRS, elbow PPT, external knee PPT, internal knee PPT, elbow CPM, CSI, and KOOS. Home-based active neurodynamic treatment has been shown to be a feasible and safe intervention for KO patients. In addition, this intervention has shown positive effects on pain and function.
Adherence to the GOLD Guidelines in Primary Care: Data from the Swiss COPD Cohort
Authors: Mangold Veronika , Boesing Maria , Berset Camille , Bridevaux Pierre-Olivier , Geiser Thomas , Joos Zellweger Ladina , Kohler Malcolm , Lüthi-Corridori Giorgia , Maier Sabrina , Miedinger David , Thurnheer Robert , von Garnier Christophe , Leuppi Jörg
Published: October 2023

(1) Introduction: Chronic obstructive pulmonary disease (COPD) and its associated morbidity and mortality are a global burden on both affected patients and healthcare systems. The Global Initiative for Chronic Obstructive Lung Disease (GOLD) issues guidelines with the aim of improving COPD management. Previous studies reported significant variability in adherence to these recommendations. The objective of this study was to evaluate Swiss primary practitioners’ adherence to the GOLD guidelines for the pharmacological treatment of stable COPD. (2) Methods: We studied patients who were included in the Swiss COPD cohort study, an ongoing prospective study in a primary care setting, between 2015 and 2022. The key inclusion criteria are age ≥ 40 years, FEV1/FVC ratio < 70%, and a smoking history of at least 20 pack-years. Adherence to the GOLD guidelines was assessed per visit and over time. (3) Results: The data of 225 COPD patients (mean age 67 ± 9 years, 64% male) and their respective 1163 visits were analyzed. In 65% of visits (726/1121), treatment was prescribed according to the GOLD guidelines. Non-adherence was most common in GOLD groups A and B (64% and 33%) and mainly consisted of over-treatment (two long-acting bronchodilators in group A (98/195, 50%) and ICS in groups A (21/195, 11%) and B (198/808, 25%)). In group D, the prescriptions conformed with the guidelines in 99% of cases (109/108). Guideline adherence was associated with high symptom load (COPD Assessment Test) (OR 1.04, p = 0.002), high number of exacerbations (OR = 2.07, p < 0.001), asthma overlap (OR 3.36, p = 0.049), and diabetes mellitus (OR 2.82, p = 0.045). (4) Conclusion: These results confirm a conflict between the GOLD recommendations and primary practice, mainly concerning over-treatment in GOLD groups A and B. Patients with high symptom load, high exacerbation risk, asthma overlap, and diabetes mellitus are more likely to be treated in conformity with the guidelines. Further research is needed to uncover the reasons for the discrepancies and to design strategies for improvement.
Estimation of Sarcopenia Indices in Women from Saudi Arabia in Relation to Menopause and Obesity: Cross-Sectional Comparative Study
Authors: Aljawini Nouf , Habib Syed Shahid
Published: October 2023

Sarcopenia prevalence depends on the definition, and ethnicity must be considered when setting reference values. However, there is no specific cut-off for sarcopenia in Saudi women. Accordingly, we aimed to establish a cut-off value for sarcopenia in Saudi women. We determined the prevalence of sarcopenia in terms of low handgrip strength (HGS) in postmenopausal women using the EWGSOP2 value, redefined a specific cut-off for low HGS derived from Saudi premenopausal women, re-determined the prevalence of low HGS using the new cut-off, and analyzed the proportion of low HGS in women with obesity compared to those without obesity. Following EWGSOP2 guidelines, we defined probable sarcopenia and set new HGS values. We assessed HGS and body composition in 134 pre/postmenopausal women. Probable sarcopenia prevalence was calculated using EWGSOP2’s HGS of 16 kg and new cut-offs from young premenopausal women without obesity. HGS 10 and 8 kg cut-offs were calculated from premenopausal Saudi women’s mean −2 SDs and mean −2.5 SDs. Using the HGS 16 kg cut-off, sarcopenia prevalence was 44% in postmenopausal and 33.89% in premenopausal women. Applying the new HGS 10 kg and 8 kg cut-offs, the prevalence was 9.33% and 4%, respectively, in postmenopausal and 5% and 3.40%, respectively, in premenopausal women. Women with obesity had a higher proportion of low HGS across all cut-offs. We suggest that EWGSOP2 cut-offs may not be adaptable for Saudi women. Considering body composition differences between Saudis and Caucasians, our proposed HGS cut-offs appear more relevant.
Subtypes of Patients with Mild to Moderate Airflow Limitation as Predictors of Chronic Obstructive Pulmonary Disease Exacerbation
Authors: Kim Nam Eun , Kang Eun-Hwa , Jung Ji Ye , Lee Chang Youl , Lee Won Yeon , Lim Seong Yong , Park Dong Il , Yoo Kwang Ha , Jung Ki-Suck , Lee Jin Hwa
Published: October 2023

COPD is a heterogeneous disease, and its acute exacerbation is a major prognostic factor. We used cluster analysis to predict COPD exacerbation due to subtypes of mild–moderate airflow limitation. In all, 924 patients from the Korea COPD Subgroup Study cohort, with a forced expiratory volume (FEV1) ≥ 50% and documented age, body mass index (BMI), smoking status, smoking pack-years, COPD assessment test (CAT) score, predicted post-bronchodilator FEV1, were enrolled. Four groups, putative chronic bronchitis (n = 224), emphysema (n = 235), young smokers (n = 248), and near normal (n = 217), were identified. The chronic bronchitis group had the highest BMI, and the one with emphysema had the oldest age, lowest BMI, and highest smoking pack-years. The young smokers group had the youngest age and the highest proportion of current smokers. The near-normal group had the highest proportion of never-smokers and near-normal lung function. When compared with the near-normal group, the emphysema group had a higher risk of acute exacerbation (OR: 1.93, 95% CI: 1.29–2.88). However, multiple logistic regression showed that chronic bronchitis (OR: 2.887, 95% CI: 1.065–8.192), predicted functional residual capacity (OR: 1.023, 95% CI: 1.007–1.040), fibrinogen (OR: 1.004, 95% CI: 1.001–1.008), and gastroesophageal reflux disease were independent predictors of exacerbation (OR: 2.646, 95% CI: 1.142–6.181). The exacerbation-susceptible subtypes require more aggressive prevention strategies.
Tear Film Stabilization and Symptom Improvement in Dry Eye Disease: The Role of Hyaluronic Acid and Trehalose Eyedrops versus Carmellose Sodium
Authors: Sánchez-González José-María , Silva-Viguera Carmen , Sánchez-González María Carmen , Capote-Puente Raúl , De-Hita-Cantalejo Concepción , Ballesteros-Sánchez Antonio , Ballesteros-Durán Lydia , García-Romera Marta-C. , Gutiérrez-Sánchez Estanislao
Published: October 2023

This study evaluated the effectiveness of hyaluronic acid and trehalose (HA/trehalose) eyedrops in managing dry eye disease (DED) symptoms by measuring tear stability and administering a DED questionnaire. Sixty patients were treated with either HA/trehalose eyedrops (Tear A) or carmellose sodium eyedrops (Tear B) as controls. The tear breakup time (TBUT) and non-invasive breakup time (NIBUT) were monitored, and patients completed the standard patient evaluation of eye dryness (SPEED) questionnaire. After two months of twice-daily applications, patients treated with the HA/trehalose eyedrops demonstrated significant improvements in the NIBUT (12.98 ± 3.22 s) and TBUT (12.95 ± 2.98 s), indicating increased tear stability. Moreover, they reported lower dry eye sensation (6.70 ± 4.94 SPEED score points), suggesting a reduction in DED symptoms. These findings underscore the efficacy of HA/trehalose eyedrops in improving both the objective and subjective signs of DED, with twice-daily application enhancing ocular surface conditions and reducing patient-reported symptoms.