bluuebunny/crossref_metadata_2025 huggingface dataset:
Anthocyanins: From Mechanisms of Regulation in Plants to Health Benefits in Foods
Authors: Cappellini Francesca , Marinelli Alessandra , Toccaceli Marta , Tonelli Chiara , Petroni Katia
Published: October 2021

Anthocyanins represent the major red, purple, and blue pigments in many flowers, fruits, vegetables, and cereals. They are also recognized as important health-promoting components in the human diet with protective effects against many chronic diseases, including cardiovascular diseases, obesity, and cancer. Anthocyanin biosynthesis has been studied extensively, and both biosynthetic and key regulatory genes have been isolated in many plant species. Here, we will provide an overview of recent progress in understanding the anthocyanin biosynthetic pathway in plants, focusing on the transcription factors controlling activation or repression of anthocyanin accumulation in cereals and fruits of different plant species, with special emphasis on the differences in molecular mechanisms between monocot and dicot plants. Recently, new insight into the transcriptional regulation of the anthocyanin biosynthesis, including positive and negative feedback control as well as epigenetic and post-translational regulation of MYB-bHLH-WD40 complexes, has been gained. We will consider how knowledge of regulatory mechanisms has helped to produce anthocyanin-enriched foods through conventional breeding and metabolic engineering. Additionally, we will briefly discuss the biological activities of anthocyanins as components of the human diet and recent findings demonstrating the important health benefits of anthocyanin-rich foods against chronic diseases.
Geographic Variation in the Petiole–Lamina Relationship of 325 Eastern Qinghai–Tibetan Woody Species: Analysis in Three Dimensions
Authors: Li Yanan , Kang Xiaomei , Zhou Jieyang , Zhao Zhigang , Zhang Shiting , Bu Haiyan , Qi Wei
Published: October 2021

The petiole–lamina relationship is central to the functional tradeoff between photosynthetic efficiency and the support/protection cost. Understanding environmental gradients in the relationship and its underlying mechanisms remains a critical challenge for ecologists. We investigated the possible scaling of the petiole–lamina relationships in three dimensions, i.e., petiole length (PL) vs. lamina length (LL), petiole cross sectional area (PCA) vs. lamina area (LA), and petiole mass (PM) vs. lamina mass (LM), for 325 Qinghai–Tibetan woody species, and examined their relation to leaf form, altitude, climate, and vegetation types. Both crossspecies analysis and meta-analysis showed significantly isometric, negatively allometric, and positively allometric scaling of the petiole–lamina relationships in the length, area, and mass dimensions, respectively, reflecting an equal, slower, and faster variation in the petiole than in the lamina in these trait dimensions. Along altitudinal gradients, the effect size of the petiole–lamina relationship decreased in the length and mass dimensions but increased in the area dimension, suggesting the importance of enhancing leaf light-interception and nutrient transport efficiency in the warm zones in petiole development, but enhancing leaf support/protection in the cold zones. The significant additional influences of LA, LM, and LA were observed on the PL–LL, PCA–LA, and PM–LM relationships, respectively, implying that the single-dimension petiole trait is affected simultaneously by multidimensional lamina traits. Relative to simple-leaved species, the presence of petiolule in compound-leaved species can increase both leaf light interception and static gravity loads or dynamic drag forces on the petiole, leading to lower dependence of PL variation on LL variation, but higher biomass allocation to the petiole. Our study highlights the need for multidimension analyses of the petiole–lamina relationships and illustrates the importance of plant functional tradeoffs and the change in the tradeoffs along environmental gradients in determining the relationships.
Adaptability to High Temperature and Stay-Green Genotypes Associated With Variations in Antioxidant, Chlorophyll Metabolism, and γ-Aminobutyric Acid Accumulation in Creeping Bentgrass Species
Authors: Li Zhou , Tang Mingyan , Hassan Muhammad Jawad , Zhang Yan , Han Liebao , Peng Yan
Published: October 2021

High temperature limits the cultivation and utilization of cool-season plants in many regions worldwide. Recently, extreme hot waves swept across the globe in summer, leading to enormous economic loss. The evaluation and identification of genotypic variation in thermotolerance within species are critical to breeding for environmental adaptation and also provide potential materials to explore thermo-resistant mechanism in plants. Forty-two accessions of creeping bentgrass (Agrostis stolonifera), which is a cool-season perennial grass for turf and ecological remediation, were collected from 15 different countries. Physiological traits, namely, chlorophyll (Chl) content, electrolyte leakage, photochemical efficiency, performance index on absorption basis, leaf relative water content, and osmotic potential were used to evaluate the heat tolerance of these materials in controlled growth chambers and field during summer. Stay-green and early-aging genotypes were selected to further reveal the potential mechanism of tolerance to senescence and heat damage associated with alterations in Chl metabolism, antioxidant and photosynthetic capacity, and endogenous γ-aminobutyric acid (GABA). Findings showed that there were significant genetic variations in physiological traits among 41 materials in response to high temperature stress. The 13M, PROVIDENCE, and LOFTS L-93 were the top three accessions with superior tolerance to heat and summer stress than other materials in terms of laboratory and field tests. In response to heat stress, the stay-green genotype PROVIDENCE exhibited significantly higher photochemical efficiency, net photosynthetic rate, transpiration rate, and water use efficiency than the heat-susceptible W6 6570. Delayed leaf senescence in relation to less Chl loss was detected in the PROVIDENCE associated with maintenance of significantly higher expression levels of Chl-anabolic genes (AsCHLH, AsPBGD, and AsPOR) and lower Chl-catabolic gene AsPPH under heat stress. Genetic attributes, such as better capacity to scavenge reactive oxygen species and higher endogenous GABA content could play positive roles in alleviating heat-induced senescence, oxidative damage, and metabolic disturbance in the PROVIDENCE.
Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence
Authors: Xie Yurong , Ma Mengdi , Liu Yang , Wang Baobao , Wei Hongbin , Kong Dexin , Wang Haiyang
Published: October 2021

Leaf senescence is the terminal stage of leaf development. Both light and the plant hormone ethylene play important roles in regulating leaf senescence. However, how they coordinately regulate leaf senescence during leaf development remains largely unclear. In this study, we show that FHY3 and FAR1, two homologous proteins essential for phytochrome A-mediated light signaling, physically interact with and repress the DNA binding activity of EIN3 (a key transcription factor essential for ethylene signaling) and PIF5 (a bHLH transcription factor negatively regulating light signaling), and interfere with their DNA binding to the promoter of ORE1, which encodes a key NAC transcription factor promoting leaf senescence. In addition, we show that FHY3, PIF5, and EIN3 form a tri-protein complex(es) and that they coordinately regulate the progression of leaf senescence. We show that during aging or under dark conditions, accumulation of FHY3 protein decreases, thus lifting its repression on DNA binding of EIN3 and PIF5, leading to the increase of ORE1 expression and onset of leaf senescence. Our combined results suggest that FHY3 and FAR1 act in an age gating mechanism to prevent precocious leaf senescence by integrating light and ethylene signaling with developmental aging.
Remote Research Methods: Considerations for Work With Children
Authors: Shields Michelle M. , McGinnis Morgan N. , Selmeczy Diana
Published: October 2021

The growing shift to online research provides numerous potential opportunities, including greater sample diversity and more efficient data collection. While online methods and recruitment platforms have gained popularity in research with adults, there is relatively little guidance on best practices for how to conduct remote research with children. The current review discusses how to conduct remote behavioral research with children and adolescents using moderated (i.e., real-time interactions between the experimenter and child) and unmoderated (i.e., independent completion of study without experimenter interaction) methods. We examine considerations regarding sample diversity and provide recommendations on implementing remote research with children, including discussions about remote software, study design, and data quality. These recommendations can promote the use of remote research amongst developmental psychologists by contributing to our knowledge of effective online research practices and helping to build standardized guidelines when working with children.
Parent-Child Attachment and Social Adaptation Behavior in Chinese College Students: The Mediating Role of School Bonding
Authors: Yin Haowen , Qian Suning , Huang Fengqiu , Zeng Huibin , Zhang Casper J. P. , Ming Wai-Kit
Published: October 2021

Family and school are two main places for adolescents to develop socialization, which can be contributed by good parent-child attachment and school bonding. Earlier studies suggested that parent-child attachment played an important role in promoting the formation of high-level school bonding, which is also likely to influence social adaptation. This study aimed to explore the relationship between parent-child attachment and social adaptation, and the mediating role of school bonding. Using stratified cluster sampling, 1,440 college students were first randomly selected from four universities and then stratified by specialty with a balance between genders and grades. Participants voluntarily participated in this study and completed questionnaires including the Parent-Child Attachment Scale, School Bonding Scale, and Social Adaptation Scale. Finally, a total of 1,320 college students were included in the analysis (59.5% female; aged 18–24years, Mage=20.39±1.52years). Data analysis and structural equation modeling were conducted using SPSS 22.0 and AMOS 23.0. The results indicated that the overall level of parent-child attachment in females (M=75.72, SD=12.36) was significantly higher than that of males (M=73.71, SD=12.68; F=8.22, p<0.01). Difference was also found between sibling status (F=13.90, p<0.001), and the only-child (M=76.16, SD=12.72) scored significantly higher than their counterparts (non-only children, M=73.60, SD=12.19). Parent-child attachment was positively correlated with social adaptation (p<0.01) and school bonding (p<0.01), while school bonding was also positively correlated with social adaptation score (p<0.01). School bonding played a partial intermediate role in the relationship between parent-child attachment and social adaptation (β=0.15). Our research identified a direct influence of parent-child attachment and an indirect influence via school bonding on social adaptation among college students.
Design and Implementation of Human-Computer Interface for Participatory Art Video Development Platform Based on Interactive Non-linear Algorithm
Authors: Liu Xixia , Liu Musen
Published: October 2021

Artificial intelligence (AI) technology is innovatively combined with participatory video for artistic creation and communication to improve the enthusiasm of art lovers for artistic creation and communication and expand the application range of AI technology. First, the interactive framework of interactive participation video is proposed based on the analysis of the related literature of interactive non-linear video. Then, a questionnaire is designed accordingly to analyze the social needs of people on art social platforms. According to the survey results, the participatory art video online communication platform is designed and preliminarily realized. Finally, a participant video eye movement control experiment is conducted to test the performance of the participatory art video development platform. Meanwhile, the platform is evaluated through field research from two aspects of test efficiency and user experience. The results show that the operation time of the participatory art video development platform is much shorter than that of the control group. It takes only approximately 15 s to complete the annotation operation with low SD, indicating that the system performance is stable. The accuracy of the platform also reaches 100%.
Corporate Social Responsibilities, Psychological Contracts and Employee Turnover Intention of SMEs in China
Authors: Hui Zhang
Published: October 2021

As an important organizational strategy and action that affects employee perception and attitude, corporate social responsibility is essential for small and medium-sized enterprises (SMEs) to reduce turnover rate and achieve sustainable growth. This paper integrates social identity theory and social exchange theory to construct an external reputation mechanism and internal trust mechanism to explore the influence mechanism of corporate social responsibility on employee turnover intention and the intermediate transmission mechanism of psychological contract. The research results show that corporate social responsibility has a significant negative impact on employee turnover intention. Compared with external corporate social responsibility, internal corporate social responsibility has a stronger negative impact on employee turnover intention; corporate social responsibility has a significant negative impact on employee transactional psychological contract, while corporate social responsibility has a significant positive impact on employee relational psychological contracts; transactional psychological contract has a significant positive effect on employee turnover intention, while relational psychological contract has a significant negative effect on employee turnover intention; psychological contract has a significant and complete mediating effect on the relationship between external corporate social responsibility and employee turnover intention, and the psychological contract plays a significant part of the intermediary role between the internal corporate social responsibility and the employee turnover intention. The conclusions enriches the complex relationship between corporate social responsibility and employee turnover intentions, and provides a reference for SMEs to effectively perform internal and external social responsibilities and reduce employee turnover rates.
Spiritual Well-Being, Social Support, and Financial Distress in Determining Depression: The Mediating Role of Impact of Event During COVID-19 Pandemic in Iran
Authors: Sharif Nia Hamid , Gorgulu Ozkan , Naghavi Navaz , Robles-Bello María Auxiliadora , Sánchez-Teruel David , Khoshnavay Fomani Fatemeh , She Long , Rahmatpour Pardis , Allen Kelly-Ann , Arslan Gokmen , Pahlevan Sharif Saeed
Published: October 2021

This study investigates the relationship between spiritual well-being, social support, and financial distress with depressive symptoms due to the COVID-19 pandemic. A path analysis was used to analyze data collected from 1,156 Iranian participants via an online survey. The results showed that spiritual well-being and social support were negatively related to depressive symptoms and financial distress. The impact of COVID-19 events showed negative associations with depressive symptoms. In addition, the link between spiritual well-being and financial distress with depressive symptoms was partially mediated by the impact of events.
Demographic and Psychographic Factors of Social Isolation During the COVID-19 Pandemic: The Importance of Technology Confidence
Authors: Horst Becky R. , Sixsmith Andrew , Simeonov Dorina , Mihailidis Alex
Published: October 2021

The COVID-19 pandemic presents an unprecedented situation in which physical distancing and “stay at home” orders have increased the pressures for social isolation. Critically, certain demographic factors have been linked to increased feelings of isolation and loneliness. These at-risk groups for social isolation may be disproportionately affected by the changes and restrictions that have been implemented to prevent viral spread. In our analysis, we sought to evaluate if perceived feelings of social isolation, during the COVID-19 pandemic, was related to demographic and technology-related psychographic characteristics. Older adults across Canada were surveyed about their demographic background, their feelings concerning confidence and proficiency in technology use, and how frequently they have felt isolated during the pandemic. In total 927 responses from Canadians over 65 years old, of varying demographic characteristics were collected. Our data shows that many older adults are feeling isolated “Often” or “Some of the time” in 2020, regardless of most demographic factors that have been previously associated with increased isolation risk. However, feelings of proficiency in using technology was an important factor affecting feelings of isolation. Given that technology proficiency is a modifiable factor, and remained significant after adjustment for demographic factors, future efforts to reduce social isolation should consider training programs for older adults to improve technology confidence, especially in an increasingly digital world.
Vegetation Angular Signatures of Equatorial Forests From DSCOVR EPIC and Terra MISR Observations
Authors: Ni Xiangnan , Knyazikhin Yuri , Sun Yuanheng , She Xiaojun , Guo Wei , Panferov Oleg , Myneni Ranga B.
Published: October 2021

In vegetation canopies cross-shading between finite dimensional leaves leads to a peak in reflectance in the retro-illumination direction. This effect is called the hot spot in optical remote sensing. The hotspot region in reflectance of vegetated surfaces represents the most information-rich directions in the angular distribution of canopy reflected radiation. This paper presents a new approach for generating hot spot signatures of equatorial forests from synergistic analyses of multiangle observations from the Multiangle Imaging SpectroRadiometer (MISR) on Terra platform and near backscattering reflectance data from the Earth Polychromatic Imaging Camera (EPIC) onboard NOAA’s Deep Space Climate Observatory (DSCOVR). A canopy radiation model parameterized in terms of canopy spectral invariants underlies the theoretical basis for joining Terra MISR and DSCOVR EPIC data. The proposed model can accurately reproduce both MISR angular signatures acquired at 10:30 local solar time and diurnal courses of EPIC reflectance (NRMSE < 9%, R2 > 0.8). Analyses of time series of the hot spot signature suggest its ability to unambiguously detect seasonal changes of equatorial forests.
CRISPR: A New Way for Scientists to Edit DNA
Authors: Marnik Elisabeth A. , Bautista Carla , Drangowska-Way Anna , Simopoulos Caitlin M. A. , Merritt Thomas J. S.
Published: October 2021

Just like humans, bacteria can get sick. Some bacteria have a defense system called CRISPR/Cas9 that protects them from infection with viruses. Over the last few years, scientists have adapted this bacterial defense system to be used in the laboratory to alter the DNA of various organisms. This article will explain how CRISPR/Cas9 is used to edit genes and will provide examples of how this technology is useful. Experiments using CRISPR/Cas9 must be carried out ethically, that is, scientists must ensure that all research respects human rights and animal welfare and complies with the law.
Implications of Climatic Stressors on Agro-Pastoral Resources Among Mbororo Communities Along the Slopes of Kilum-Ijim Mountain, North West Region, Cameroon
Authors: Kongnso Moye Eric , Buba Umaru Hassan , Nfor Julius Tata
Published: October 2021

Mountains are rich in pastures and water for agro-pastoral resources and supports rain-fed farming that sustain the livelihoods of many indigenous communities. This work seeks to examine the implications of climatic variability on agro-pastoral resources (pasture land, water) and food security within the Mbororo communities. To ascertain this, 350 household questionnaires were randomly administered in four Mbororo Ardorates and in-depth interviews conducted with local authorities. Quantitative and qualitative analysis of data collected revealed that the link between climate, land and water is paramount in animal rearing and crop farming in mountains. Cattle rearing and crop farming have been the main source of livelihoods for about 90% of the Mbororos as they depend on it for food and income. Climatic perturbations characterized by frequent dry spells, rainfall anomalies and other environmental stressors predicted degrading pastoral resources and the independent variable explained the outcome variable at R = 0.787; R2 = 0. 623; ΔR2 = 0.622; p < 0.01. This implies that 62.3% of degradation is accounted for by environmental stressors. As such, the carrying capacity of grazing have gone above the authorized number of two cattle per hectare, leading to overgrazing and degradation. Encroachments into grazing lands by crop farmers, invasion by unproductive grass species and farmer-grazer conflicts are aggravated by climatic stressors. The Fundong council and traditional authorities of the Kom Fondom have been working together to demarcate grazing land and provide water for cattle rearing. Mbororo communities are equally diversifying their activities to ensure food availability.
Tannic Acid-Steeped Corn Grain Modulates in vitro Ruminal Fermentation Pattern and Microbial Metabolic Pathways
Authors: Zhao F. F. , Zhang X. Z. , Zhang Y. , Elmhadi Mawda , Qin Y. Y. , Sun H. , Zhang H. , Wang M. Z. , Wang H. R.
Published: October 2021

This study investigated the effects of tannic acid (TA)-treated corn on changes in ruminal fermentation characteristics and the composition of the ruminal bacterial community in vitro. Ruminal fluid was obtained from three rumen-fistulated goats fed a 60:40 (forage/concentrate) diet. The batch cultures consisted of 25 ml of strained rumen fluid in 25 ml of an anaerobic buffer containing 0.56 g of ground corn, 0.24 g of soybean meal, 0.10 g of alfalfa, and 0.10 g of oat grass. Ground corn (2 mm) was steeped in an equal quantity (i.e., in a ratio of 1:1, w/v) of water alone (Con), 15 (TA15), 25 (TA25), and 35 g/l (TA35) TA solution for 12 h. After incubation for 24 h, TA-treated corn linearly increased (P <0.05) ruminal pH and the molar proportion of acetate, but linearly reduced (P <0.05) total volatile fatty acids and the molar proportion of butyrate compared with the Con treatment. Illumina MiSeq sequencing was used to investigate the profile changes of the ruminal microbes. A principal coordinates analysis plot based on weighted UniFrac values revealed that the structure of the ruminal bacterial communities in the control group was different from that of the TA-treated corn groups. The results of changes in the rumen bacterial communities showed that TA-treated corn linearly enriched (P <0.05) Rikenellaceae_RC9_gut_group, but linearly reduced (P <0.05) Ruminococcaceae_NK4A214_group, Ruminococcus_2, and unclassified_o__Clostridiales. Functional prediction of ruminal microbiota revealed that the TA-treated corn linearly decreased ruminal microbiota function of utilizing starch through pyruvate metabolism. In conclusion, TA-treated corn can modulate the rumen fermentation characteristics, microbial composition, and metabolic pathways, which may be potentially useful for preventing the occurrence of ruminal acidosis.
What Are the Human Resources Required to Control a Foot-and-Mouth Disease Outbreak in Austria?
Authors: Marschik Tatiana , Kopacka Ian , Stockreiter Simon , Schmoll Friedrich , Hiesel Jörg , Höflechner-Pöltl Andrea , Käsbohrer Annemarie , Conrady Beate
Published: October 2021

Contingency planning allows veterinary authorities to prepare a rapid response in the event of a disease outbreak. A recently published foot-and-mouth disease (FMD) simulation study indicated concerns whether capacity was sufficient to control a potential FMD epidemic in Austria. The objectives of the study presented here were to estimate the human resources required to implement FMD control measures and to identify areas of the operational activities that could potentially delay successful control of the disease. The stochastic spatial simulation model EuFMDiS (The European Foot-and-Mouth Disease Spread Model) was used to simulate a potential FMD outbreak and its economic impact, including different control scenarios based on variations of culling, vaccination, and pre-emptive depopulation. In this context, the utilization of human resources was assessed based on the associated EuFMDiS output regarding the performance of operational activities. The assessments show that the number of personnel needed in an outbreak with a stamping-out policy would reach the peak at the end of the second week of control with a median of 540 (257–926) individuals, out of which 31% would be veterinarians. Approximately 58% of these human resources would be attributable to surveillance, followed by staff for cleaning and disinfection activities. Our analysis demonstrates that, of the operational activities, surveillance personnel were the largest factor influencing the magnitude of the outbreak. The aim of the assessment presented here is to assist veterinary authorities in the contingency planning of required human resources to respond effectively to an outbreak of animal diseases such as FMD.
Change in Colorectal Cancer Tests Submitted for Reimbursement in Switzerland 2012–2018: Evidence from Claims Data of a Large Insurance
Authors: Schneider Rémi , Näpflin Markus , Syrogiannouli Lamprini , Bissig Sarah , Tal Kali , Bulliard Jean-Luc , Ducros Cyril , Senn Oliver , Selby Kevin , Bähler Caroline , Blozik Eva , Auer Reto
Published: October 2021

Objectives:Guidelines recommend colorectal cancer (CRC) screening by fecal occult blood test (FOBT) or colonoscopy. In 2013, Switzerland introduced reimbursement of CRC screening by mandatory health insurance for 50-69-years-olds, after they met their deductible. We hypothesized that the 2013 reimbursement policy increased testing rate.

Methods:In claims data from a Swiss insurance, we determined yearly CRC testing rate among 50-75-year-olds (2012–2018) and the association with socio-demographic, insurance-, and health-related covariates with multivariate-adjusted logistic regression models. We tested for interaction of age (50–69/70–75) on testing rate over time.

Results:Among insurees (2012:355′683; 2018:348′526), yearly CRC testing rate increased from 2012 to 2018 (overall: 8.1–9.9%; colonoscopy: 5.0–7.6%; FOBT: 3.1–2.3%). Odds ratio (OR) were higher for 70–75-year-olds (2012: 1.16, 95%CI 1.13–1.20; 2018: 1.05, 95%CI 1.02–1.08). Deductible interacted with changes in testing rate over time (p< 0.001). The increase in testing rate was proportionally higher among 50-69-years-olds than 70-75-year-olds over the years.

Conclusions:CRC testing rate in Switzerland increased from 2012 to 2018, particularly among 50-69-years-olds, the target population of the 2013 law. Future studies should explore the effect of encouraging FOBT or waiving deductible.
Turbidity and Chemical Oxygen Demand Reduction from Pig Slurry through a Coagulation Flocculation Process
Authors: El bied Oumaima , Kessler Mathieu , Terrero Martire Angélica , Fechtali Taoufiq , Cano Angel Faz , Acosta José A.
Published: October 2021

Pig slurry is considered a high-risk effluent that causes several environmental problems if it is not adequately managed and treated. White Iberian pig farms in the southeast of Spain treat their slurry in situ using separation, double filtration, decantation, and constructed wetland treatments. However, the pretreatment process does not successfully reduce solids, which leads to clogging in the constructed wetlands (CWs). The main objective of this research paper is to reduce the turbidity and chemical oxygen demand (COD) from the effluent to make it appropriate for CW treatment. Optimization of the coagulation–flocculation (CF) process using iron chloride and a cationic flocculent DKFLOCC-1598 was investigated by a central composite design method (CCD). The effects of coagulant concentration, pH, and flocculent on the COD and turbidity removal were evaluated. The best results were found using 0.024 mol L−1 iron chloride and 0.164 mL L−1 flocculent at pH 7.5, which reduced COD by 96% and delivered turbidity removal of 97%. Therefore, the results indicate the high efficiency of the treatment method in reducing the COD and suspended solids.
The Effect of Nitrogen Fertilization on Yield and Macronutrient Concentrations in Three Cultivars of Jerusalem Artichoke (Helianthus tuberosus L.)
Authors: Wierzbowska Jadwiga , Cwalina-Ambroziak Bożena , Bogucka Bożena
Published: October 2021

In many countries, Jerusalem artichoke (JA) is a source of biomass for renewable energy production and alternative biofuel feedstock, and it is used for feed and food production. The species also has medicinal properties, and it is used in soil reclamation. The aim of this study was to evaluate the effect of N fertilization on the yield and macronutrient concentrations in JA tubers. The effect of N fertilization (control plot, unfertilized, 80 and 120 kg ha−1) on aerial biomass yield, tuber yield, and the mineral composition of tubers in three JA cultivars (“cv.”) (Rubik, Albik, and Gute Gelbe) was investigated in a field experiment. Tuber yield (40.99 Mg ha−1) and aerial biomass yield (62.76 Mg ha−1) were highest in cv. Gute Gelbe fertilized with 120 kg N ha−1 in the warm and moderately wet growing season of 2018. Agronomic N-use efficiency (AE) was highest in cv. Gute Gelbe. In the treatment supplied with 80 kg N ha−1, the fresh matter yield (FMY) of tubers was determined at 66.4 kg kg−1 N, whereas in the treatment fertilized with 120 kg N ha−1, the FMY of tubers reached 101.8 kg kg−1 N. The evaluated JA cultivars differed in their responses to an increase in the N fertilizer (marginal efficiency—ME) rate from 80 to 120 kg ha−1. The strongest response was observed in cv. Gute Gelbe, where the tuber yield increased by 172.6 kg kg−1 N. The tubers of cv. Gute Gelbe were characterized by significantly higher concentrations of N, K, Mg, and S compared with the other cultivars. The concentrations of macronutrients in the tubers (without Mg) were higher in spring. Nitrogen fertilization did not cause differences in the concentrations of P, K, Ca, Mg, and S, but it increased the N concentration in tubers.
High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey
Authors: Upadhyay Anamica , Shahzad Anwar , Ahmad Zishan , Alatar Abdulrahman A. , Guerriero Gea , Faisal Mohammad
Published: October 2021

Diplocyclos palmatus (L.) C. Jeffrey, commonly referred to as “Shivalingi” or “Lollipop climber” is a valuable medicinal plant with a climbing growth habit used in traditional medicine. It is reputed to have antiarthritic, anti-diabetic properties and to be useful in various skin and reproductive problems. Overexploitation of wild plants and low seed germination have resulted in the decline of the species in the wild. Thus, the present investigation was aimed to establish an effective in vitro propagation procedure for its large-scale production and conservation. Nodal explants, obtained from an established mother plant were grown on MS basal medium augmented with various cytokinins, alone or in combination with auxins, to study the morphogenic response. A maximum of 8.3 shoots/explants with an average shoot length of 7.2 cm were produced after six weeks on MS containing benzylaminopurine 5.0 µM + 1-naphthaleneacetic acid 2.0 µM. After 4 weeks of transfer, microshoots rooted well on a low nutrient medium of ½ MS + 1.0 µM indole-3-butyric acid, with a maximum of 11.0 roots/microshoot and an average root length of 7.4 cm. With an 80% survival rate, the regenerated plantlets were effectively acclimatized to natural conditions. DNA-based molecular markers were used to investigate the genetic uniformity. Scanning Electron Microscopic examination of leaves indicated the adaptation of the plantlets to natural, as evidenced by the formation of normal stomata. Gas chromatography-mass spectrometry analyses of mother and micropropagated plants were performed to identify essential secondary metabolites. The results obtained show that the in vitro propagation system can be adopted for preservation, large-scale production and secondary metabolites’ production in D. palmatus.
Short Communication: Obesity Intervention Resulting in Significant Changes in the Human Gut Viral Composition
Authors: Sandoval-Vargas Diego , Concha-Rubio Nataly D. , Navarrete Paola , Castro Matías , Medina Daniel A.
Published: October 2021

Obesity is a health problem of global concern that negatively impacts quality of life. Various studies have implicated obesity in the disruption of the normal microbiome composition. The virome consists of a collection of all the viruses that inhabit a particular niche. However, the study of such viruses that compose the human gut microbiome in the context of obesity has been paid little attention. One interesting aspect of virome study is the description of phages that can specifically interact with the bacterial component of the microbiota and modulate the microbiome’s dynamics. Previous work showed that the microbiota composition changes after obesity treatment and that these changes are country specific. In this work, we perform a quick gut viral composition of the gut microbiota of patients from Denmark, Italy, and Chile before and after obesity intervention using metagenomic sequences previously published and bioinformatics tools. Our results showed that obesity treatment results in significant changes in the human gut viral composition. These results conclude that the virome composition changes after obesity intervention by suggesting that changes can be related to the microbiota rearrangements reported in other works and may be involved in microbial dynamics after treatment.
Predicting ICD-9 Codes Using Self-Report of Patients
Authors: Singaravelan Anandakumar , Hsieh Chung-Ho , Liao Yi-Kai , Hsu Jia-Lien
Published: October 2021

The International Classification of Diseases (ICD) is a globally recognized medical classification system that aids in the identification of diseases and the regulation of health trends. The ICD framework makes it easy to keep track of records and evaluate medical data for evidence-based decision-making. Several methods have predicted ICD-9 codes based on the discharge summary, clinical notes, and nursing notes. In our study, our approach only utilizes the subjective component to predict ICD-9 codes. Data cleaning and segmentation, and Natural Language Processing (NLP) techniques are applied on the subjective component during the pre-processing. Our study builds the Long Short-Term Memory (LSTM) and the Gated Recurrent Unit (GRU) to develop a model for predicting ICD-9 codes. The ICD-9 codes contain different ICD levels such as chapter, block, three-digit code, and full code. The GRU model scores the highest recall of 57.91% in the chapter level and the top-10 experiment has a recall of 67.37%. Based on the subjective component, the model can help patients in the form of a remote assistance tool.
A Thermal Discomfort Index for Demand Response Control in Residential Water Heaters
Authors: Porteiro Rodrigo , Chavat Juan , Nesmachnow Sergio
Published: October 2021

Demand-response techniques are crucial for providing a proper quality of service under the paradigm of smart electricity grids. However, control strategies may perturb and cause discomfort to clients. This article proposes a methodology for defining an index to estimate the discomfort associated with an active demand management consisting of the interruption of domestic electric water heaters. Methods are applied to build the index include pattern detection for estimating the water utilization using an Extra Trees ensemble learning method and a linear model for water temperature, both based on analysis of real data. In turn, Monte Carlo simulations are applied to calculate the defined index. The proposed approach is evaluated over one real scenario and two simulated scenarios to validate that the thermal discomfort index correctly models the impact on temperature. The simulated scenarios consider a number of households using water heaters to analyze and compare the thermal discomfort index for different interruptions and the effect of using different penalty terms for deviations of the comfort temperature. The obtained results allow designing a proper management strategy to fairly decide which water heaters should be interrupted to guarantee the lower discomfort of users.
Classical and Quantum Gases on a Semiregular Mesh
Authors: De Gregorio Davide , Prestipino Santi
Published: October 2021

The main objective of a statistical mechanical calculation is drawing the phase diagram of a many-body system. In this respect, discrete systems offer the clear advantage over continuum systems of an easier enumeration of microstates, though at the cost of added abstraction. With this in mind, we examine a system of particles living on the vertices of the (biscribed) pentakis dodecahedron, using different couplings for first and second neighbor particles to induce a competition between icosahedral and dodecahedral orders. After working out the phases of the model at zero temperature, we carry out Metropolis Monte Carlo simulations at finite temperature, highlighting the existence of smooth transitions between distinct “phases”. The sharpest of these crossovers are characterized by hysteretic behavior near zero temperature, which reveals a bottleneck issue for Metropolis dynamics in state space. Next, we introduce the quantum (Bose-Hubbard) counterpart of the previous model and calculate its phase diagram at zero and finite temperatures using the decoupling approximation. We thus uncover, in addition to Mott insulating “solids”, also the existence of supersolid “phases” which progressively shrink as the system is heated up. We argue that a quantum system of the kind described here can be realized with programmable holographic optical tweezers.
Studying the Effect of Chondroitin Sulfate on the Physicochemical Properties of Novel Gelatin/Chitosan Biopolymer-Based Cryogels
Authors: Kudaibergen Gulshakhar , Zhunussova Madina , Mun Ellina A. , Arinova Anar , Ogay Vyacheslav
Published: October 2021

The application of biopolymers in tissue engineering is of a great interest due to of their inherent properties such as cell adhesion, biodegradation, bioavailability, and viscoelasticity. In this study, we synthesized cryogels based on biopolymers of gelatin, chitosan, and chondroitin sulfate by cryopolymerization and studied the effect of chondroitin sulfate on changing the physicochemical properties of cryogels such as pore size, pore volume, density, gel fraction, and biodegradation. A macroporous surface of the synthesized polymers has been investigated by SEM. The glass transition temperatures of the crosslinked cryogels, determined by the DSC method, were higher compared to that of the non-crosslinked cryogel used as a reference. The results of the MTT test showed that aqueous extracts of the prepared cryogels had no toxic effect on rat adipose-derived mesenchymal stem cells. The research in this area is of great importance and provides new insights into novel, effective methods for obtaining biopolymers that can be used as carriers of cells.
Fabricating Sustainable All-Cellulose Composites
Authors: Uusi-Tarkka Eija-Katriina , Skrifvars Mikael , Haapala Antti
Published: October 2021

Climate change, waste disposal challenges, and emissions generated by the manufacture of non-renewable materials are driving forces behind the production of more sustainable composite materials. All-cellulose composites (ACCs) originate from renewable biomass, such as trees and other plants, and are considered fully biodegradable. Dissolving cellulose is a common part of manufacturing ACCs, and currently there is a lot of research focused on effective, but also more environmentally friendly cellulose solvents. There are several beneficial properties of ACC materials that make them competitive: light weight, recyclability, low toxicity, good optical, mechanical, and gas barrier properties, and abundance of renewable plant-based raw material. The most prominent ACC applications are currently found in the food packing, medical, technical and vehicle industries. All-cellulose nanocomposites (ACNCs) expand the current research field and can offer a variety of more specific and functional applications. This review provides an overview of the manufacture of sustainable ACCs from lignocellulose, purified cellulose, and cellulosic textiles. There is an introduction of the cellulose dissolution practices of creating ACCs that are currently researched, the structure of cellulose during complete or partial dissolution is discussed, and a brief overview of factors which influence composite properties is presented.
Design and Validation of a Virtual Chemical Laboratory—An Example of Natural Science in Elementary Education
Authors: Tsai Chi-Yi , Ho Yu-Chen , Nisar Humaira
Published: October 2021

In the natural science curriculum, chemistry is a very important domain. However, when conducting chemistry experiments, safety issues need to be taken seriously, and excessive material waste may be caused during the experiment. Based on the 11-year-old student science curriculum, this paper proposed a virtual chemistry laboratory, which was designed by combining a virtual experiment application with physical teaching materials. The virtual experiment application was a virtual experiment laboratory environment created by using selected experimental equipment cards in combination with augmented reality (AR) technology. The physical teaching materials included all virtual equipment required for experiment units. Each piece of equipment had corresponding cards for learners to choose from and utilize in specific experimental operations. It was hoped that students were able to achieve the desired learning effectiveness of experimental teaching while reducing the waste of experimental materials through the virtual experimental environment. This study employed the quasi-experimental and questionnaire survey methods to evaluate both learning effectiveness and learning motivation. Eighty-one students and eight elementary school teachers were surveyed as research subjects. The experimental results revealed that significant differences in learning effectiveness existed between the experimental group and control group, indicating that the application of AR technology to teaching substantively helped enhance students’ learning effectiveness and motivation. In addition, the results of the teacher questionnaire demonstrated that the virtual chemistry laboratory proposed in this study could effectively assist with classroom teaching.
Oral Impacts of Aligners versus Fixed Self-Ligating Lingual Orthodontic Appliances
Authors: Angelopoulos Gerassimos G. , Kanarelis Panagiotis , Vagdouti Georgia , Zavlanou Ageliki , Sifakakis Iosif
Published: October 2021

The aim of this prospective study was to compare a fixed lingual orthodontic appliance with a commonly used aligner system, focusing on oral impacts and speech disturbances, during the first 3 months of orthodontic treatment. Two groups of adults were evaluated: 21 treated with Invisalign® and 26 with In-Ovation L® lingual brackets. Health-related quality of life questionnaires were used to assess the oral impacts, and speech samples were collected for speech evaluation by professionals. The levels of pain and painkiller intake were similar, increasing on the first day, and decreasing at 3 months. Subtle injuries on the lip and cheeks were initially reported in the aligner group. Lingual patients experienced more discomfort in swallowing and opening the mouth, tongue injuries and food residues, even at 3 months. The consonants “s” and “z” were mainly affected in both groups immediately after appliance insertion, but more often in the aligner group. These speech disturbances recovered in the following 3 months. In conclusion, both groups reported disturbances in quality-of-life measures; however, patients wearing lingual appliances experienced more disturbances, which persisted after three months. Speech disturbances were slightly more pronounced in the aligner group and recovered after three months.
Nebulization of Low-Dose S-Nitrosoglutathione in Diabetic Stroke Enhances Benefits of Reperfusion and Prevents Post-Thrombolysis Hemorrhage
Authors: Zaidi Syed Kashif , Ahmed Farid , Alkhatabi Heba , Hoda Md Nasrul , Al-Qahtani Muhammad
Published: October 2021

The COVID-19 pandemic has escalated the occurrence of hypoxia including thrombotic stroke worldwide, for which nitric oxide (NO) therapy seems very promising and translatable. Therefore, various modes/routes of NO-delivery are now being tested in different clinical trials for safer, faster, and more effective interventions against ischemic insults. Intravenous (IV) infusion of S-Nitrosoglutathione (GSNO), the major endogenous molecular pool of NO, has been reported to protect against mechanical cerebral ischemia-reperfusion (IR); however, it has been never tested in any kind of “clinically” relevant thromboembolic stroke models with or without comorbidities and in combination with the thrombolytic reperfusion therapy. Moreover, “IV-effects” of higher dose of GSNO following IR-injury have been contradicted to augment stroke injury. Herein, we tested the hypothesis that nebulization of low-dose GSNO will not alter blood pressure (BP) and will mitigate stroke injury in diabetic mice via enhanced cerebral blood flow (CBF) and brain tissue oxygenation (PbtO2). GSNO-nebulization (200 μg/kgbwt) did not alter BP, but augmented the restoration of CBF, improved behavioral outcomes and reduced stroke injury. Moreover, GSNO-nebulization increased early reoxygenation of brain tissue/PbtO2 as measured at 6.5 h post-stroke following thrombolytic reperfusion, and enervated unwanted effects of late thrombolysis in diabetic stroke. We conclude that the GSNO-nebulization is safe and effective for enhancing collateral microvascular perfusion in the early hours following stroke. Hence, nebulized-GSNO therapy has the potential to be developed and translated into an affordable field therapy against ischemic events including strokes, particularly in developing countries with limited healthcare infrastructure.
Assessment and Rehabilitation of Culturally Protected Prince Rudolf Infantry Barracks in Zagreb after Major Earthquake
Authors: Milić Mija , Stepinac Mislav , Lulić Luka , Ivanišević Nataša , Matorić Ivan , Čačić Šipoš Boja , Endo Yohei
Published: October 2021

Recently, Zagreb was struck by a strong earthquake. Damage throughout the city was tremendous due to numerous aged and vulnerable masonry buildings. Many damaged buildings are under a certain level of cultural heritage protection. Hence, reliable assessment and effective rehabilitation are important to preserve cultural significance and mitigate risk for human life. With that in mind, the procedure of a detailed condition assessment of the building under heritage protection is presented. A detailed historical background of the case study building is shown, and observed damage and conducted in situ tests are discussed. The nonlinear static seismic analysis performed in the 3Muri software is extensively elaborated. Four different levels of reconstruction according to new Croatian law are briefly presented. Additionally, several strengthening scenarios are proposed with various strengthening techniques.