bluuebunny/crossref_metadata_2025 huggingface dataset:
Endophytic Bacteria Colonizing the Petiole of the Desert Plant Zygophyllum dumosum Boiss: Possible Role in Mitigating Stress
Authors: Srinivasan Jansirani , Khadka Janardan , Novoplansky Nurit , Gillor Osnat , Grafi Gideon
Published: February 2022

Zygophyllum dumosum is a dominant shrub in the Negev Desert whose survival is accomplished by multiple mechanisms including abscission of leaflets to reduce whole plant transpiration while leaving the fleshy, wax-covered petioles alive but dormant during the dry season. Petioles that can survive for two full growing seasons maintain cell component integrity and resume metabolic activity at the beginning of the winter. This remarkable survival prompted us to investigate endophytic bacteria colonizing the internal tissues of the petiole and assess their role in stress tolerance. Twenty-one distinct endophytes were isolated by culturing from surface-sterile petioles and identified by sequencing of the 16S rDNA. Sequence alignments and the phylogenetic tree clustered the isolated endophytes into two phyla, Firmicutes and Actinobacteria. Most isolated endophytes displayed a relatively slow growth on nutrient agar, which was accelerated by adding petiole extracts. Metabolic analysis of selected endophytes showed several common metabolites whose level is affected by petiole extract in a species-dependent manner including phosphoric acid, pyroglutamic acid, and glutamic acid. Other metabolites appear to be endophyte-specific metabolites, such as proline and trehalose, which were implicated in stress tolerance. These results demonstrate the existence of multiple endophytic bacteria colonizing Z. dumosum petioles with the potential role in maintaining cell integrity and functionality via synthesis of multiple beneficial metabolites that mitigate stress and contribute to stress tolerance.
The Response of Spore Germination of Sphagnum Mosses to Single and Combined Fire-Related Cues
Authors: Yusup Shuayib , Sundberg Sebastian , Fan Beibei , Sulayman Mamtimin , Bu Zhao-Jun
Published: February 2022

Plants in flammable ecosystems have different response strategies to fire, such as increasing germination after exposure to smoke and break of dormancy through heat shock. Peatlands are ecosystems that are more likely to be disturbed by fire with increasing temperatures, but it is not clear how fire affects spore germination of Sphagnum, the dominant plants in peatlands. Here, we hypothesize that Sphagnum spores respond positively to single and combined treatments of moderate heat and smoke (by increased germinability), while spore germinability decreases in response to high temperature. We exposed the Sphagnum spores of four selected species (S. angustifolium, S. fuscum, S. magellanicum and S. squarrosum) collected from peatlands in the Changbai Mountains to heat (40, 60 and 100 °C), on its own and combined with smoke-water treatments. Our results showed that a heat of 100 °C inhibited the spore germination or even killed spores of all species, while spore germination of three (Sphagnumangustifolium, S. fuscum and S. squarrosum) of the four species was promoted by 40 and 60 °C heat compared to the control (20 °C). Hollow species (S. angustifolium and S. squarrosum) showed a stronger positive responsive to heat than hummock species (S. fuscum and S. magellanicum). Sphagnumfuscum spores responded positively to the combined heat and smoke treatment while the other species did not. For the first time, we demonstrate the positive effects of heat on its own and in combination with smoke on spore germination in wetland mosses, which may be important for the establishment and persistence of peatmoss populations after fire.
Development and Validation of a One-Step Reverse Transcription Real-Time PCR Assay for Simultaneous Detection and Identification of Tomato Mottle Mosaic Virus and Tomato Brown Rugose Fruit Virus
Authors: Tiberini Antonio , Manglli Ariana , Taglienti Anna , Vučurović Ana , Brodarič Jakob , Ferretti Luca , Luigi Marta , Gentili Andrea , Mehle Nataša
Published: February 2022

Tobamovirus species represent a threat to solanaceous crops worldwide, due to their extreme stability and because they are seed borne. In particular, recent outbreaks of tomato brown rugose fruit virus in tomato and pepper crops led to the establishment of prompt control measures, and the need for reliable diagnosis was urged. Another member of the genus, tomato mottle mosaic virus, has recently gained attention due to reports in different continents and its common features with tomato brown rugose fruit virus. In this study, a new real-time RT-PCR detection system was developed for tomato brown rugose fruit virus and tomato mottle mosaic virus on tomato leaves and seeds using TaqMan chemistry. This test was designed to detect tomato mottle mosaic virus by amplifying the movement protein gene in a duplex assay with the tomato brown rugose fruit virus target on the CP-3’NTR region, which was previously validated as a single assay. The performance of this test was evaluated, displaying analytical sensitivity 10−5–10−6-fold dilution for seeds and leaves, respectively, and good analytical specificity, repeatability, and reproducibility. Using the newly developed and validated test, tomato brown rugose fruit virus detection was 100% concordant with previously performed analyses on 106 official samples collected in 2021 from different continents.
Genetic Control of Efficient Nitrogen Use for High Yield and Grain Protein Concentration in Wheat: A Review
Authors: Teng Wan , He Xue , Tong Yiping
Published: February 2022

The increasing global population and the negative effects of nitrogen (N) fertilizers on the environment challenge wheat breeding to maximize yield potential and grain protein concentration (GPC) in an economically and environmentally friendly manner. Understanding the molecular mechanisms for the response of yield components to N availability and assimilates allocation to grains provides the opportunity to increase wheat yield and GPC simultaneously. This review summarized quantitative trait loci/genes which can increase spikes and grain number by enhancing N uptake and assimilation at relative early growth stage, and 1000-grain weight and GPC by increasing post-anthesis N uptake and N allocation to grains.
Integrated Physiological and Metabolomic Analyses of the Effect of Potassium Fertilizer on Citrus Fruit Splitting
Authors: Jiao Yun , Sha Cunlong , Shu Qiaoyun
Published: February 2022

Potassium (K) nutrition plays a key role in alleviating a variety of peel disorders in tree fruit, but the effect of this nutrient on the physiological and metabolic profiles involved in the fruit splitting of citrus remains unclear. Three levels of K were used to treat citrus ‘Ehime Kashi 34’ (Citrus Nishinoka × C. Shiranui), a hybrid cultivar with fruit that easily split. The results showed that the roots of the treatment with K fertilizer increased the contents of calcium (Ca2+), nitrogen (N), and K in the skin and flesh, the fruit firmness ratio of the peel to the flesh, photosynthetic rate, stomatal conductance, and concentration of intercellular CO2. In contrast, it decreases the relative chlorophyll index and content of Ca2+ in the leaves. Simultaneously, 59 and 13 differentially expressed metabolites (DEMs) were detected in the peel and flesh, respectively, after treatment with K. Of them, five compounds were upregulated, including the synthesis of various amino acids in the peel and the accumulation of various glycoside metabolites in the flesh which were upregulated. The accumulation of levels of gibberellin and glycoside were downregulated. That could be the main reason why potassium alleviates fruit splitting.
In Vitro Technology in Plant Conservation: Relevance to Biocultural Diversity
Authors: Kulak Verena , Longboat Sheri , Brunet Nicolas D. , Shukla Mukund , Saxena Praveen
Published: February 2022

Plant diversity is critical to the functioning of human societies, and evidence shows that plant conservation success is driven by integrative approaches that include social and biological factors. Plants have a unique capacity to reproduce asexually, and propagation practices can yield large numbers of plantlets. These plantlets can be used in several ways to fulfil conservation goals including the repopulation of regions with declining densities of threatened species that hold cultural meaning. However, the potential of in vitro technologies in the conservation of plants that hold cultural meaning is understudied. In this paper we focus upon the roles of in vitro technologies in the conservation of plants relevant to biocultural environments and provide an overview of potential knowledge gaps at the interface of in vitro and plants used traditionally, including those meaningful to Indigenous Peoples. We conclude that in vitro technologies can be powerful tools in biocultural conservation if they are deployed in a manner respectful of the socio-cultural context in which plants play a role, but that further research is needed in this regard. We suggest several epistemological points to facilitate future research.
Flip-Flap: A Simple Dual-View Imaging Method for 3D Reconstruction of Thick Plant Samples
Authors: Serra Leo , Tan Sovanna , Robinson Sarah , Langdale Jane A.
Published: February 2022

Plant development is a complex process that relies on molecular and cellular events being co-ordinated in space and time. Microscopy is one of the most powerful tools available to investigate this spatiotemporal complexity. One step towards a better understanding of complexity in plants would be the acquisition of 3D images of entire organs. However, 3D imaging of intact plant samples is not always simple and often requires expensive and/or non-trivial approaches. In particular, the inner tissues of thick samples are challenging to image. Here, we present the Flip-Flap method, a simple imaging protocol to produce 3D images of cleared plant samples at the organ scale. This method allows full 3D reconstruction of plant organs suitable for 3D segmentation and further related analysis and can be easily handled by relatively inexperienced microscopists.
Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro
Authors: Wang Jiao , Cao Ke , Wang Lirong , Dong Wenxuan , Zhang Xiao , Liu Weisheng
Published: February 2022

Anthocyanins are important pigments in peach fruit and are beneficial to human health. Sugars are both energy-storing and signaling molecules and their roles in inducing anthocyanin biosynthesis have received a great deal of research attention. However, the mechanism by which sugars induce anthocyanin biosynthesis in peach fruit is unknown. In order to understand this induction mechanism, comprehensive transcriptome and metabolome were performed in fruit flesh treated with four different sugars for 12 and 24 h, respectively. Here, we found that cyanidin-3-O-(6-O-p-coumaroyl) glucosides accumulated in fruit flesh treated with glucose, sucrose, sorbitol, and fructose in vitro. Two key structural genes of the anthocyanin biosynthesis pathway, namely, PpDFR and PpUFGT, were upregulated in the flesh of sugar-treated peach fruit. By contrast, the two main transcription factors (TFs) PpMYB10.1 and PpBL regulating anthocyanin biosynthetic genes in peach fruit were not upregulated accordingly. Interestingly, two MYB family genes (PpMYB6 and PpMYB44-like) and three bHLH family genes (PpbHLH35, PpbHLH51, and PpbHLH36-like) were upregulated. A dual luciferase assay revealed that PpMYB6 strongly activated the PpUFGT promoter when it was co-infiltrated with PpbHLH35, PpbHLH51, and PpbHLH36-like. When PpMYB44-like was co-infiltrated with PpbHLH35, it also potently activated the PpUFGT promoter. The results of this study help clarify the molecular mechanisms by which glucose, sucrose, sorbitol, and fructose regulate anthocyanin accumulation in peach fruit.
Novel Mixed Matrix Membranes Based on Polyphenylene Oxide Modified with Graphene Oxide for Enhanced Pervaporation Dehydration of Ethylene Glycol
Authors: Dmitrenko Mariia , Chepeleva Anastasia , Liamin Vladislav , Mazur Anton , Semenov Konstantin , Solovyev Nikolay , Penkova Anastasia
Published: February 2022

Ethylene glycol (EG) is widely used in various economic and industrial fields. The demand for its efficient separation and recovery from water is constantly growing. To improve the pervaporation characteristics of a poly(2,6-dimethyl-1,4-phenylene oxide) (PPO) membrane in dehydration of ethylene glycol, the modification with graphene oxide (GO) nanoparticles was used. The effects of the introduction of various GO quantities into the PPO matrix on the structure and physicochemical properties were studied by Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopies, scanning electron (SEM) and atomic force (AFM) microscopies, thermogravimetric analysis (TGA), swelling experiments, and contact angle measurements. Two types of membranes based on PPO and PPO/GO composite were developed: dense membranes and supported membranes on a fluoroplast substrate (MFFC). Transport properties of the developed membranes were evaluated in the pervaporation dehydration of EG in a wide concentration range (10–90 wt.% and 10–30 wt.% water for the dense and supported membranes, respectively). The supported PPO/GO(0.7%)/MFFC membrane demonstrated the best transport properties in pervaporation dehydration of EG (10–30 wt.% water) at 22 °C: permeation flux ca. 15 times higher compared to dense PPO membrane—180–230 g/(m2·h)), 99.8–99.6 wt.% water in the permeate. The membrane is suitable for the promising industrial application.
Problems with Applying the Ozawa–Avrami Crystallization Model to Non-Isothermal Crosslinking Polymerization
Authors: Vyazovkin Sergey , Galukhin Andrey
Published: February 2022

Ozawa has modified the Avrami model to treat non-isothermal crystallization kinetics. The resulting Ozawa–Avrami model yields the Avrami index (n) and heating/cooling function (χ(T)). There has been a number of recent applications of the Ozawa–Avrami model to non-isothermal crosslinking polymerization (curing) kinetics that have determined n and have used χ(T) in place of the rate constant (k(T)) in the Arrhenius equation to evaluate the activation energy (E) and the preexponential factor (A). We analyze this approach mathematically as well as by using simulated and experimental data, highlighting the following problems. First, the approach is limited to the processes that obey the Avrami model. In cases of autocatalytic or decelerating kinetics, commonly encountered in crosslinking polymerizations, n reveals a systematic dependence on temperature. Second, χ(T) has a more complex temperature dependence than k(T) and thus cannot produce exact values of E and A via the Arrhenius equation. The respective deviations can reach tens or even hundreds of percent but are diminished dramatically using the heating/cooling function in the form [χ(T)]1/n. Third, without this transformation, the Arrhenius plots may demonstrate breakpoints that leads to questionable interpretations. Overall, the application of the Ozawa–Avrami model to crosslinking polymerizations appears too problematic to be justified, especially considering the existence of well-known alternative kinetic techniques that are flexible, accurate, and computationally simple.
One-Pot Synthesis of Amphiphilic Biopolymers from Oxidized Alginate and Self-Assembly as a Carrier for Sustained Release of Hydrophobic Drugs
Authors: Liu Zhaowen , Chen Xiuqiong , Huang Zhiqin , Wang Hongcai , Cao Shirui , Liu Chunyang , Yan Huiqiong , Lin Qiang
Published: February 2022

In this paper, we developed an organic solvent-free, eco-friendly, simple and efficient one-pot approach for the preparation of amphiphilic conjugates (Ugi-OSAOcT) by grafting octylamine (OCA) to oxidized sodium alginate (OSA). The optimum reaction parameters that were obtained based on the degree of substitution (DS) of Ugi-OSAOcT were a reaction time of 12 h, a reaction temperature of 25 °C and a molar ratio of 1:2.4:3:3.3 (OSA:OCA:HAc:TOSMIC), respectively. The chemical structure and composition were characterized by Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (1H NMR), X-ray diffraction (XRD), thermogravimetry analyser (TGA), gel permeation chromatography (GPC) and elemental analysis (EA). It was found that the Ugi-OSAOcT conjugates with a CMC value in the range of 0.30–0.085 mg/mL could self-assemble into stable and spherical micelles with a particle size of 135.7 ± 2.4–196.5 ± 3.8 nm and negative surface potentials of −32.8 ± 0.4–−38.2 ± 0.8 mV. Furthermore, ibuprofen (IBU), which served as a model poorly water-soluble drug, was successfully incorporated into the Ugi-OSAOcT micelles by dialysis method. The drug loading capacity (%DL) and encapsulation efficiency (%EE) of the IBU-loaded Ugi-OSAOcT micelles (IBU/Ugi-OSAOcT = 3:10) reached as much as 10.9 ± 0.4–14.6 ± 0.3% and 40.8 ± 1.6–57.2 ± 1.3%, respectively. The in vitro release study demonstrated that the IBU-loaded micelles had a sustained and pH-responsive drug release behavior. In addition, the DS of the hydrophobic segment on an OSA backbone was demonstrated to have an important effect on IBU loading and drug release behavior. Finally, the in vitro cytotoxicity assay demonstrated that the Ugi-OSAOcT conjugates exhibited no significant cytotoxicity against RAW 264.7 cells up to 1000 µg/mL. Therefore, the amphiphilic Ugi-OSAOcT conjugates synthesized by the green method exhibited great potential to load hydrophobic drugs, acting as a promising nanocarrier capable of responding to pH for sustained release of hydrophobic drugs.
Development of Natural Fibre-Reinforced Semi-Finished Products with Bio-Based Matrix for Eco-Friendly Composites
Authors: Möhl Claudia , Weimer Timo , Caliskan Metin , Baz Stephan , Bauder Hans-Jürgen , Gresser Götz T.
Published: February 2022

Increasing resource consumption and a growing amount of textile waste increase the importance of a circular economy and recycling in the fashion and apparel industry. Environmentally friendly bio-based composites made from cellulosic fibres obtained from textile waste, and polymers based on renewable raw materials present a possible solution. In this study, the development of textile semi-finished products based on medium-to-long cotton and flax fibres obtained from textile waste in combination with a bio-based thermoplastic matrix for lightweight applications is investigated. For the production of natural fibre-polylactide hybrid yarns, fibre slivers with improved fibre orientation and blending are produced. Subsequently, quasi-unidirectional woven fabrics are produced and consolidated into bio-based composites. Textile and mechanical properties of hybrid yarns as well as bio-composites are analysed with regard to the influence of fibre length, fibre distribution in the yarn, yarn structure and fibre volume content. The results show that the production of bio-based semi-finished products can be a potential way for upcycling textile waste.
Recent Advances in the Synthesis of Complex Macromolecular Architectures Based on Poly(N-vinyl pyrrolidone) and the RAFT Polymerization Technique
Authors: Roka Nikoletta , Kokkorogianni Olga , Kontoes-Georgoudakis Philippos , Choinopoulos Ioannis , Pitsikalis Marinos
Published: February 2022

Recent advances in the controlled RAFT polymerization of complex macromolecular architectures based on poly(N-vinyl pyrrolidone), PNVP, are summarized in this review article. Special interest is given to the synthesis of statistical copolymers, block copolymers, and star polymers and copolymers, along with graft copolymers and more complex architectures. In all cases, PNVP is produced via RAFT techniques, whereas other polymerization methods can be employed in combination with RAFT to provide the desired final products. The advantages and limitations of the synthetic methodologies are discussed in detail.
Fabrication of Ropivacaine/Dexamethasone-Eluting Poly(D, L-lactide-co-glycolide) Microparticles via Electrospraying Technique for Postoperational Pain Control
Authors: Shen Shih-Jyun , Chou Ying-Chao , Hsu Shih-Chieh , Lin Yu-Ting , Lu Chia-Jung , Liu Shih-Jung
Published: February 2022

Microencapsulation plays an important role in biomedical technology owing to its particular and attractive characteristics. In this work, we developed ropivacaine and dexamethasone loaded poly(D, L-lactide-co-glycolide) (PLGA) microparticles via electrospraying technique and investigated the release behavior of electrosprayed microparticles. The particle morphology of sprayed particles was assessed using scanning electron microscopy (SEM). The in vitro drug release kinetics were evaluated employing an elution method, and the in vivo pharmaceutical release as well as its efficacy on pain relief were tested using an animal activity model. The microscopic observation suggested that sprayed microparticles exhibit a size distribution of 5–6 µm. Fourier-transform infrared spectrometry and differential scanning calorimetry demonstrated the successful incorporation of pharmaceuticals in the PLGA particulates. The drugs-loaded particles discharged sustainably high concentrations of ropivacaine and dexamethasone at the target region in vivo for over two weeks, and the drug levels in the blood remained low. By adopting the electrospraying technique, we were able to prepare drug-embedded polymeric microparticles with effectiveness and with a sustainable capability for postoperative pain control.
Angiopep-2-Modified Nanoparticles for Brain-Directed Delivery of Therapeutics: A Review
Authors: Habib Saffiya , Singh Moganavelli
Published: February 2022

Nanotechnology has opened up a world of possibilities for the treatment of brain disorders. Nanosystems can be designed to encapsulate, carry, and deliver a variety of therapeutic agents, including drugs and nucleic acids. Nanoparticles may also be formulated to contain photosensitizers or, on their own, serve as photothermal conversion agents for phototherapy. Furthermore, nano-delivery agents can enhance the efficacy of contrast agents for improved brain imaging and diagnostics. However, effective nano-delivery to the brain is seriously hampered by the formidable blood–brain barrier (BBB). Advances in understanding natural transport routes across the BBB have led to receptor-mediated transcytosis being exploited as a possible means of nanoparticle uptake. In this regard, the oligopeptide Angiopep-2, which has high BBB transcytosis capacity, has been utilized as a targeting ligand. Various organic and inorganic nanostructures have been functionalized with Angiopep-2 to direct therapeutic and diagnostic agents to the brain. Not only have these shown great promise in the treatment and diagnosis of brain cancer but they have also been investigated for the treatment of brain injury, stroke, epilepsy, Parkinson’s disease, and Alzheimer’s disease. This review focuses on studies conducted from 2010 to 2021 with Angiopep-2-modified nanoparticles aimed at the treatment and diagnosis of brain disorders.
Lignocellulosic Based Biochar Adsorbents for the Removal of Fluoride and Arsenic from Aqueous Solution: Isotherm and Kinetic Modeling
Authors: Ayaz Iram , Rizwan Muhammad , Ullman Jeffery Layton , Haroon Hajira , Qayyum Abdul , Ahmed Naveed , Elesawy Basem H. , Askary Ahmad El , Gharib Amal F. , Ismail Khadiga Ahmed
Published: February 2022

Eucalyptus wood is made up of lignocellulosic material; this lignocellulosic material contains two types of biopolymers, i.e., carbohydrate and aromatic polymers. In this study, this lignocellulosic material was used to prepare biochar. Three biochar, i.e., laboratory-based (B1), barrel-based (B2), and brick kiln-biochar (B3), were used for fluoride and arsenic removal from aqueous solution. Barrel-based biochar was prepared by using the two-barrel method’s alteration. The highest fluoride removal (99%) was attained at pH 2 in the presence of B1, while in the presence of B2 and B3, maximum fluoride removal was 90% and 45.7%, respectively. At pH 10, the maximum arsenic removal in the presence of B1, B2, and B3 was 96%, 94%, and 93%, respectively. The surface characteristics obtained by Fourier-transform infrared spectroscopy (FTIR) showed the presence of carbonyl group (C-O), and alkene (C=C) functional groups on all the three studied biochars. Isotherm studies showed that the adsorption was monolayered (all the adsorbed molecules were in contact with the surface layer of the adsorbent) as the Langmuir isotherm model best fits the obtained data. Adsorption kinetics was also performed. The R2 value supports the pseudo-second-order kinetics, which means that chemisorption was involved in adsorbing fluoride and arsenic. It is concluded that B1 gives maximum removal for both fluoride (99%) and arsenic (96%). The study shows that lignocellulose-based biochar can be used for arsenic and fluoride removal from water.
Polymer-Based Wound Dressing Materials Loaded with Bioactive Agents: Potential Materials for the Treatment of Diabetic Wounds
Authors: Alven Sibusiso , Peter Sijongesonke , Mbese Zintle , Aderibigbe Blessing A.
Published: February 2022

Diabetic wounds are severe injuries that are common in patients that suffer from diabetes. Most of the presently employed wound dressing scaffolds are inappropriate for treating diabetic wounds. Improper treatment of diabetic wounds usually results in amputations. The shortcomings that are related to the currently used wound dressings include poor antimicrobial properties, inability to provide moisture, weak mechanical features, poor biodegradability, and biocompatibility, etc. To overcome the poor mechanical properties, polymer-based wound dressings have been designed from the combination of biopolymers (natural polymers) (e.g., chitosan, alginate, cellulose, chitin, gelatin, etc.) and synthetic polymers (e.g., poly (vinyl alcohol), poly (lactic-co-glycolic acid), polylactide, poly-glycolic acid, polyurethanes, etc.) to produce effective hybrid scaffolds for wound management. The loading of bioactive agents or drugs into polymer-based wound dressings can result in improved therapeutic outcomes such as good antibacterial or antioxidant activity when used in the treatment of diabetic wounds. Based on the outstanding performance of polymer-based wound dressings on diabetic wounds in the pre-clinical experiments, the in vivo and in vitro therapeutic results of the wound dressing materials on the diabetic wound are hereby reviewed.
The Importance of Identity Style for the Level of Religiosity in Different Developmental Periods
Authors: Gurba Ewa , Czyżowska Dorota , Topolewska-Siedzik Ewa , Cieciuch Jan
Published: February 2022

Identity is built in the context of an individual’s professed values, moral principles, and often, religiosity. Adolescence and emerging and early adulthood are times of intensive identity construction and of changes in religious attitudes. Therefore, the aim of this study was to characterise people across these three developmental periods in terms of the level of their identity and religiosity, and to examine whether particular identity styles allow for the prediction of the overall level of religiosity. For this purpose, the whole sample of N = 1017 individuals and particularly from adolescence (n = 307), emerging adulthood (n = 410), and early adulthood (n = 302) were studied. The results showed a lower level of the informational style and a higher level of the diffuse-avoidant style in adolescence as compared with the two older groups, who did not differ from each other. The overall level of religiosity did not significantly differentiate the developmental groups; however, it was explained by identity formation styles in different ways at particular developmental stages. Results of moderation analyses suggest that the informational style has positive effect only in adolescence; the normative style has positive effects in each age group but is strongest in early adulthood, and the diffuse-avoidant style presents negative effect in adolescence.
Methodology and Mysticism: For an Integral Study of Religion
Authors: Völker Fabian
Published: February 2022

By means of a paradigmatic investigation of the subjective, interior side of mysticism, this article intends to contribute to the methodological debate within religious studies. By tracing the possibilities of empirical access to their limit, it will be shown that the study of religion cannot possibly do without recourse to a phenomenological mode of access of its material and without philosophical reflection on its significance if it wants to do full justice to its distinctive object of research in its most essential features. The holistic approach urged here, requires, as its constitutive basis, an integrative methodology, one that is in principle able to combine all fruitful lines of inquiry in a methodically differentiated and reflexively judicious manner and, thus, to allow each of the complementary ways of looking to have their own legitimacy respected as they unfold their specific questions. Seeking a robust support for the methodological pluralism of an integral study of religion, which will keep it from succumbing to the empiricist reductionism of the cultural studies perspective, I propose that a transcendental philosophical method should be considered as a basis. Furthermore, this empowers a critical expansion and deepening of new approaches to the phenomenology of religion and a constructive interaction with the intercultural philosophy of religion.
God’s Benevolent Love in the Time of COVID-19 Pandemic: Articulations and Experiences of Select Filipino Youth
Authors: Cacho Rebecca , del Castillo Fides
Published: February 2022

Current studies show that the Christian faith is still robust in Philippine society and culture despite the global health crisis. This study investigates how select Filipino Christian youths intuited God’s benevolent love in the time of “social distancing” and how their experience of God’s benevolence helped them to carry on despite the COVID-19 pandemic. The authors administered open-ended survey questions about God’s love to select Filipino Christian youths. The responses were analyzed using thematic analysis and categorizations. Using contextual analysis, the researchers critically reflected upon the respondents’ notions of God’s love and religious experiences from the two poles of Filipino culture and Judeo-Christian tradition. According to select Filipino Christian youths, God is present and faithful. God’s “kagandahang-loob” (benevolent love) for human persons is evinced in the care and kindness of family members and neighbors. God also enables human persons to prevail over challenges in life, such as the COVID-19 pandemic. Moreover, God is the ultimate source of Christian hope. This hope is firmly anchored in Jesus Christ and the Church. Furthermore, God is forgiving and merciful. He continues to bless and give graces to human persons despite their sinfulness. These contemporary conceptualizations of God’s perfect interior goodness helped the select Filipino Christian youths cope with the COVID-19 pandemic.
An Object-Oriented Approach to the Classification of Roofing Materials Using Very High-Resolution Satellite Stereo-Pairs
Authors: Trevisiol Francesca , Lambertini Alessandro , Franci Francesca , Mandanici Emanuele
Published: February 2022

The availability of multispectral images, with both high spatial and spectral resolution, makes it possible to obtain valuable information about complex urban environment, reducing the need for more expensive surveying techniques. Here, a methodology is tested for the semi-automatic extraction of buildings and the mapping of the main roofing materials over a urban area of approximately 100 km2, including the entire city of Bologna (Italy). The methodology follows an object-oriented approach and exploits a limited number of training samples. After a validation based on field inspections and close-range photos acquired by a drone, the final map achieved an overall accuracy of 94% (producer accuracy 79%) regarding the building extraction and of 91% for the classification of the roofing materials. The proposed approach proved to be flexible enough to catch the strong variability of the urban texture in different districts and can be easily reproducible in other contexts, as only satellite imagery is required for the mapping.
Long-Term Temporal and Spatial Monitoring of Cladophora Blooms in Qinghai Lake Based on Multi-Source Remote Sensing Images
Authors: Duan Hongyu , Yao Xiaojun , Zhang Dahong , Jin Huian , Wei Qixin
Published: February 2022

With climate warming and intensification of human activities, the eco-environmental problems of lakes in middle and high latitudes become increasingly prominent. Qinghai Lake, located in the northeastern of the Tibetan Plateau, is the largest inland saltwater lake in China. Recently, the problem of Cladophora blooms has been widely concerning. In this study, the area of floating Cladophora blooms (hereafter FCBs) in Qinghai Lake from 1986 to 2021 was extracted using Floating Algal Index (FAI) method based on Landsat TM/ETM+/OLI and Sentinel-2 MSI images, and then the intra- and inter-annual variation characteristics and spatial patterns of FCBs were analyzed. The results show that the general change trend of FCBs in Qinghai Lake featured starting in May, expanding rapidly from June to August, and increasing steadily from September to October. From 1986 to 2021, the area of FCBs in Qinghai Lake showed an overall increasing trend in all months, with the largest increase in July at 0.1 km2/a, followed by October at 0.096 km2/a. Spatially speaking, the FCBs area showed a significant increasing trend in the northern Buha River estuary (BRN) and southern Buha River estuary (BRS) regions, a slight increase in the Shaliu River estuary (SR) region, and a decreasing trend in the Quanji River estuary (QR) region and the Heima River estuary (HR) region. The correlation between the meteorological factors and the changes in FCBs was weak, but the increase in flooded pastures in the BRN region (Bird Island) due to rising water levels was definitely responsible for the large-scale increase in FCBs in this region. However, the QB, northeastern bay of Shaliu River estuary (SRB) and HR regions, which also have extensive inundated grassland, did not have the same increase in FCBs area, suggesting that the growth of Cladophora is caused by multiple factors. The complex relationships need to be verified by further research. The current control measures have a certain inhibitory effect on the Cladophora bloom in Qinghai Lake because the FCBs area was significantly smaller in 2017–2020 (5.22 km2, 3.32 km2, 4.55 km2 and 2.49 km2), when salvage work was performed, than in 2016 and 2021 (8.67 km2 and 9.14 km2), when no salvage work was performed.
Remotely Sensed Spatiotemporal Variation in Crude Protein of Shortgrass Steppe Forage
Authors: Irisarri Jorge Gonzalo N. , Durante Martin , Derner Justin D. , Oesterheld Martin , Augustine David J.
Published: February 2022

In the Great Plains of central North America, sustainable livestock production is dependent on matching the timing of forage availability and quality with animal intake demands. Advances in remote sensing technology provide accurate information for forage quantity. However, similar efforts for forage quality are lacking. Crude protein (CP) content is one of the most relevant forage quality determinants of individual animal intake, especially below an 8% threshold for growing animals. In a set of shortgrass steppe paddocks with contrasting botanical composition, we (1) modeled the spatiotemporal variation in field estimates of CP content against seven spectral MODIS bands, and (2) used the model to assess the risk of reaching the 8% CP content threshold during the grazing season for paddocks with light, moderate, or heavy grazing intensities for the last 22 years (2000–2021). Our calibrated model explained up to 69% of the spatiotemporal variation in CP content. Different from previous investigations, our model was partially independent of NDVI, as it included the green and red portions of the spectrum as direct predictors of CP content. From 2000 to 2021, the model predicted that CP content was a limiting factor for growth of yearling cattle in 80% of the years for about 60% of the mid-May to October grazing season. The risk of forage quality being below the CP content threshold increases as the grazing season progresses, suggesting that ranchers across this rangeland region could benefit from remotely sensed CP content to proactively remove yearling cattle earlier than the traditional October date or to strategically provide supplemental protein sources to grazing cattle.
A No-Reference Edge-Preservation Assessment Index for SAR Image Filters under a Bayesian Framework Based on the Ratio Gradient
Authors: Ma Xiaoshuang , Hu Hongming , Wu Penghai
Published: February 2022

Denoising is an essential preprocessing step for most applications using synthetic aperture radar (SAR) images at different processing levels. Besides suppressing the noise, a good filter should also effectively preserve the image edge information. To quantitatively assess the edge-preservation performance of SAR filters, a number of indices have been investigated in the literature; however, most of them do not fully employ the statistical traits of the SAR image. In this paper, we review some of the typical edge-preservation assessment indices. A new referenceless index is then proposed. The ratio gradient is utilized to characterize the difference between two non-overlapping neighborhoods on opposite sides of each pixel in both the speckled and despeckled images. Based on these gradients and the statistical traits of the speckle, the proposed indicator is derived under a Bayesian framework. A series of experiments conducted with both simulated and real SAR datasets reveal that the proposed index shows good performances, in both robustness and consistency. For reproducibility, the source codes of the index and the testing datasets are provided.
The Impact of Vegetation on the Visibility of Archaeological Features in Airborne Laser Scanning Datasets from Different Acquisition Dates
Authors: Doneus Michael , Banaszek Łukasz , Verhoeven Geert J.
Published: February 2022

Digital elevation models derived from airborne laser scanning have found worldwide application in archaeology and other disciplines. A key feature that makes these models so valuable lies in their capacity to represent micro-relief features indicating traces of past human activity. While detection of these often faint traces in vegetated areas benefits from maximum leaf-off conditions during data acquisition, countrywide collection of data must make compromises and often cannot take place in the most appropriate seasons. In this paper, we identify the impact of leaf-on conditions on the distribution of ground returns and present what types of archaeological objects might remain unnoticeable if the flight date is outside the desirable time window. Comparing five ALS data acquisition campaigns from both leaf-off (April and November) and leaf-on conditions (May and June), we demonstrate how foliage affects the morphology of relief features as recorded in ALS derivatives, and we identify other effects on archaeological interpretation caused by changing vegetation conditions. The results encourage evaluation of countrywide general-purpose data for their applicability in archaeology.
Evaluation of foF2 and hmF2 Parameters of IRI-2016 Model in Different Latitudes over China under High and Low Solar Activity Years
Authors: Zhang Bingbing , Wang Zhengtao , Shen Yi , Li Wang , Xu Feng , Li Xiaoxiao
Published: February 2022

The height of the peak electron density (hmF2) and the critical frequency of the F2 layer (foF2) are very important in the research of ionospheric electrodynamics and high frequency (HF) wireless communication. In the article, we validated the hmF2/foF2 model values of the latest version of the International Reference Ionosphere (IRI-2016) with observations from three ionosonde stations which belong to low, middle, and high latitudes (i.e., Sanya, Beijing and Mohe) over China during a high solar activity year (2014, F10.7 = 145.9 sfu) and a low solar activity year (2016, F10.7 = 88.7 sfu). Among them, foF2 model values can be obtained through the International Radio Consulting Committee (CCIR) model or the International Union of Radio Science (URSI) model, both of which have the “F-peak storm model” on or ‘off’ options; hmF2 model values can be obtained through Bilitza-Sheikh-Eyfrig (BSE-1979), Altadill-Magdaleno-Torta-Blanch (AMTB-2013), or SHUbin (SHU-2015) model. The IRI-2016 hmF2/foF2 model values were evaluated by root mean square (RMS) values and mean absolute relative error (MARE). The results show that for the foF2 parameter, the performance of IRI-2016 can be improved by choosing “F-peak storm model” on option in geomagnetic-disturbed days. Whether in high or low solar activity years, for foF2, the IRI-2016 options of CCIR have better prediction ability than IRI-2016 options of URSI in low and high latitudes over China, and the IRI-2016 options of URSI have better prediction ability than IRI-2016 options of URSI in middle latitudes. For hmF2, the IRI-2016 option of SHU-2015 has better prediction ability than the IRI-2016 options of AMTB-2013 and BSE-1949 in high latitudes over China, the IRI-2016 options of SHU-2015 and BSE-1979 have better prediction ability than IRI-2016 options of AMTB-2013 in mid and low latitudes over China.
Close Observation of the Evolution Process during Initial Stage of Triggered Lightning Based on Continuous Interferometer
Authors: Chen Zefang , Zhang Yang , Fan Yanfeng , Wang Jingxuan , Lyu Weitao , Zheng Dong , Pang Wenjing
Published: February 2022

The discharge signal in the initial stage of lightning is weak. The revelation of the discharge mechanism at this stage depends especially on close observation. In this study, a continuous interferometer (CINTF) was used to observe the initial stage of the upward positive leader (UPL) of the triggered lightning in Conghua District, Guangzhou City, Guangdong Province. The positioning error of CINTF for a close-range radiation source was analyzed, and the positioning error calibration method of CINTF for a specific close-range radiation source was studied, which improved the observation accuracy of elevation angle at the initial stage of the UPL of the triggered lightning. With the rise of the rocket, the positioning error in altitude during the initial stage of the UPL increased obviously. Under the layout condition of the Guangzhou field experiment site for lightning research, when the positioning results of the elevation angle of the initial stage of the UPL were 40°, 50°, and 60°, respectively, the calibrated altitude positioning error could be reduced by about 11 m, 14 m, and 20 m, respectively. On the basis of the calibrated observation results, the evolution characteristics of the initial stage of the UPL were studied, and its discharge mechanism was revealed. The precursor current pulse (PCP) was generated by a weak upward positive breakdown and a subsequent strong downward negative breakdown near the rising rocket tip, which was in the form of a single pulse. The precursor current pulse cluster (PCP cluster) and initial precursor current pulse cluster (IPCP) were both signs of self-sustaining development of the UPL. After the PCP cluster, self-sustaining development stopped immediately. The self-sustaining development after IPCP could be short-term or continuous.
Complex Ecosystem Impact of Rapid Expansion of Industrial and Mining Land on the Tibetan Plateau
Authors: Liu Qionghuan , Wang Xiuhong , Zhang Yili , Li Shicheng
Published: February 2022

The ecological security of the Tibetan Plateau is vital for sustainable development. In recent years, biodiversity loss and ecosystem degradation caused by industrial and mining activities have attracted wide attention. However, a synthesis assessment of the impacts of industrial and mining land (IML) on the ecosystem is currently lacking. In this study, based on the land cover data and normalized differential vegetation index, we used the landscape ecological index, Theil-Sen trend analysis and equivalent value factors method to evaluate the change in IML and its ecosystem impact on the TP. The results demonstrated that the area of IML expanded by 3.3 times (228.56%) during 1990–2020, and reached 968.95 km² in 2020. Within this area, the newly added, stable, and reduced areas were 842.71, 126.26, and 168.65 km², respectively. Simultaneously, IML expansion made the landscape more fragmented during 1990–2020. The number of patches, splitting index, and landscape shape index in 2020 increased by 3.59-, 2.70-, and 1.90-fold compared to those in 1990, respectively. Furthermore, the difference in the vegetation change between the IML and its 10 km buffer zone was significant. About 77.34% of the vegetation in the IML area showed a trend of decrease, while about 76.51% of the vegetation in the buffer zone of IML showed a trend of increase. In addition, the expansion of IML also reduced the total ecological services value by USD 6969.31 million (0.66%) from 1990 to 2020. However, the lowered value was USD 8649.50 million (0.82%) in the newly added IML. This study highlights the rapid expansion of IML and reveals the ecosystem structure, ecosystem quality, and ecosystem service impact on the TP, which helps guide ecosystem protection and the sustainable development of mining.
Systematic Propagation of AVHRR AOD Uncertainties—A Case Study to Demonstrate the FIDUCEO Approach
Authors: Popp Thomas , Mittaz Jonathan
Published: February 2022

The AVHRR aerosol optical depth (AOD) is inverted from measured reflectances in the red band using a statistical correlation of surface reflectance with mid-infrared channel reflectances and a modelling climatology of the aerosol type. For such a sensor not specifically designed for AOD retrieval, propagating uncertainties is crucial because the sensitivity of the retrieved AOD to the measured signal varies largely with retrieval conditions (AOD itself, surface brightness, aerosol optical properties/aerosol type, observing geometry). In order to quantify the different contributions to the AOD uncertainties, we have undertaken a thorough analysis of the retrieval operator and its sensitivities to the used input and auxiliary variables. Uncertainties are then propagated from measured reflectances to geophysical retrieved AOD datasets at the super-pixel level and further to gridded daily and monthly products. The propagation uses uncertainty correlations of separate uncertainty contributions from the FIDUCEO easyFCDR level1b products (common fully correlated, independent random, and structured parts) and estimated uncertainty correlation structures of other major effects in the retrieval (surface brightness, aerosol type ensemble, cloud mask). The pixel-level uncertainties are statistically validated against true error estimates versus AERONET ground-based AOD measurements. It is shown that a 10-year time record over Europe compares well to a merged multi-satellite record and that pixel-level uncertainties provide a meaningful representation of error distributions. The study demonstrates the benefits of new recipes for uncertainty characterization from the Horizon-2020 project FIDUCEO (“Fidelity and uncertainty in climate data records from Earth Observations”) and extends them further with recent additions developed within the ESA Climate Change Initiative.