bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding the Responses of Soil Bacterial Communities to Long-Term Fertilization Regimes Using DNA and RNA Sequencing
Authors: Li Juan , Wen Yanchen , Yang Xiangdong
Published: November 2021

Studies of soil DNA-based and RNA-based bacterial communities under contrasting long-term fertilization regimes can provide valuable insights into how agricultural management affects soil microbial structure and functional diversity. In this study, soil bacterial communities subjected to six fertility treatments in an alkaline soil over 27 years were investigated by 454 pyrosequencing based on 16S rDNA and 16S rRNA. Long-term fertilization showed significant influences on the diversity of the soil DNA-based bacteria, as well as on their RNA-based members. The top five phyla (Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria, and Planctomycetes) were found in both the DNA- and RNA-based samples. However, the relative abundances of these phyla at both DNA and RNA levels were showed significantly different. Analysis results showed that the diversity of the 16S rRNA samples was consistently lower than that of the rDNA samples, however, 16S rRNA samples had higher relative abundance. PICRUSt analysis indicated that glycan biosynthesis and metabolism were detected mainly in the DNA samples, while metabolism and degradation of xenobiotics and the metabolism of amino acids, terpenoids and polyketides were relatively higher in the RNA samples. Bacilli were significantly more abundant in all the OM-fertilized soils. Redundancy analysis indicated that the relative abundances of both DNA- and RNA-based bacterial groups were correlated with soil total organic carbon content, nitrogen content, Olsen-P, and soil pH. Moreover, the RNA-based Bacilli were positively correlated with available phosphorus (Olsen-P).
A New Method to Compare the Interpretability of Rule-Based Algorithms
Authors: Margot Vincent , Luta George
Published: November 2021

Interpretability is becoming increasingly important for predictive model analysis. Unfortunately, as remarked by many authors, there is still no consensus regarding this notion. The goal of this paper is to propose the definition of a score that allows for quickly comparing interpretable algorithms. This definition consists of three terms, each one being quantitatively measured with a simple formula: predictivity, stability and simplicity. While predictivity has been extensively studied to measure the accuracy of predictive algorithms, stability is based on the Dice-Sorensen index for comparing two rule sets generated by an algorithm using two independent samples. The simplicity is based on the sum of the lengths of the rules derived from the predictive model. The proposed score is a weighted sum of the three terms mentioned above. We use this score to compare the interpretability of a set of rule-based algorithms and tree-based algorithms for the regression case and for the classification case.
Study on the Viability of Canine Nose Pattern as a Unique Biometric Marker
Authors: Choi Hyeong In , Kim Mu-Young , Yoon Hun-Young , Lee Sungjin , Choi Stephanie Sujin , Han Chang Yong , Moon Hwan Pyo , Byun Changhyun , Kwon Song-Hwa
Published: November 2021

The uniqueness of the canine nose pattern was studied. A total of 180 nose images of 60 dogs of diverse age, gender, and breed were collected. The canine nose patterns in these images were examined visually and by a biometric algorithm. It was found that the canine nose pattern remains invariant regardless of when the image is taken; and that the canine nose pattern is indeed unique to each dog. The same study was also performed on an enlarged dataset of 278 nose images of 70 dogs of 19 breeds. The study of the enlarged dataset also leads to the same conclusion. The result of this paper confirms and enhances the claims of earlier works by others that the canine nose pattern is indeed unique to each animal and serves as a unique biometric marker.
Integrity of Sperm Cell Membrane in the Semen of Crossbred and Purebred Boars during Storage at 17 °C: Heterosis Effects
Authors: Wysokińska Anna , Szablicka Dorota
Published: November 2021

The aim of the study was to assess changes in the integrity of sperm cell membranes during the storage of semen collected from Duroc × Pietrain crossbred boars and purebred boars of the component breeds. To compare the cell membrane integrity of sperm heads in crossbred and purebred boars, heterosis effects were estimated. The study was conducted on 48 ejaculates collected from Duroc × Pietrain crossbred boars and from purebred Duroc and Pietrain boars used for artificial insemination. Microscope slides were prepared from each ejaculate for the evaluation of the cell membrane integrity of the sperm, at 1, 24, 48, 72, and 96 h after collection of the ejaculate. Diluted ejaculates were stored at 17 °C. Sperm membrane integrity was analysed by two methods: SYBR-14/PI and eosin–nigrosin. Our results showed that the cell membrane integrity of sperm heads changed with storage time, but the extent of the changes varied depending on the genetic group of boars. The semen of Duroc × Pietrain crossbreds was clearly seen to be less sensitive to storage conditions than that of boars of the parent breeds, which was confirmed by the calculated heterosis effects. The percentage of sperm with an intact cell membrane was higher in crossbred boars than in purebred boars (p ≤ 0.05). In addition, significantly fewer moribund sperm spermatozoa and spermatozoa with a damaged cell membrane were observed in crossbred boars (p ≤ 0.05). In the semen of purebred Duroc and Pietrain boars, the cell membrane integrity of the sperm should be assessed more often during storage than in the semen of Duroc × Pietrain crossbred boars. This study provides valuable information for the development and implementation of semen quality monitoring in crossbred boars and boars of the parent breeds during storage at 17 °C with respect to the cell membrane structure of sperm heads. The evaluation methods used effectively identify damage to the cell membranes of the sperm during semen storage.
Immunomodulatory Effect of Vitamin C on Proinflammatory Cytokines Production in Ossimi Lambs (Ovis aries) with Pneumonic Pasteurellosis
Authors: Rizk Mohamed Abdo , El-Sayed Shimaa Abd El-Salam , Salman Doaa , Marghani Basma H. , Gadalla Hossam Elshahat , Sayed-Ahmed Mohamed Z.
Published: November 2021

In this study, we have investigated the impact of vitamin C on the production of pro-inflammatory cytokines (interleukin 1 β (IL-1 β), interleukin 6 (IL-6), interleukin 12p40 (IL-12p40), interferon gamma (IFNγ), and tumor necrosis factor alpha (TNF-α)) in lambs naturally infected by pneumonic pasteurellosis. Of 37 lambs, 18 lambs were identified to have pneumonic pasteurellosis and randomly allocated into two equal groups. Single subcutaneous dose of tulathromycine alone (2.5 mg kg−1) or tulathromycine combined with vitamin C (3 gm kg−1) were administrated to the diseased lambs. The serum levels of IL-1β, IL-6, IFN-γ, and TNF-α were returned to the normal levels in pneumonic lambs treated with the combination therapy. The obtained results indicate the selective influences of vitamin C on pro-inflammatory cytokines production in sera of lambs with pneumonic pasteurellosis and highlights the value of vitamin C as a potential anti-inflammatory drug and ideal immunomodulatory agent.
A Social-Emotional Learning Program for Suicide Prevention through Animal-Assisted Intervention
Authors: Muela Alexander , Balluerka Nekane , Sansinenea Eneko , Machimbarrena Juan Manuel , García-Ormaza Jon , Ibarretxe Nekane , Eguren Ane , Baigorri Patxi
Published: November 2021

The aim of the study was to carry out a pilot implementation and evaluation of the OverCome-AAI program, a pioneering program for the prevention of suicidal behavior through animal-assisted interventions for young people with high risk factors for suicidal behavior. The study sample consisted of 30 adolescents (11 boys and 19 girls) aged between 14 and 17 years (Mean age = 15.50, SD = 1.60) from the Basque Country (Northern Spain). After the intervention, subjects presented reductions in suicidal ideation, suicide plans, and non-suicidal self-harm, as well as a greater predisposition to seek help. A reduction in the intensity of mental pain was also found, although no differences were observed in indicators of hopelessness and depression. The preliminary results obtained in this pilot study suggest that the OverCome-AAI program may be effective in reducing suicidal behavior and non-suicidal self-harm in young people in residential care who present high risk factors for suicide.
Over Time Decay of Cortisol Metabolites in Faecal Pellets of Koalas in Central Queensland
Authors: Santamaria Flavia , Schlagloth Rolf , Palme Rupert , Henning Joerg
Published: November 2021

Faecal material can be a valuable source of information for a range of animal health aspects and can be used to measure faecal cortisol metabolites (FCMs). FCM values can relate to physiological stress responses. However, freshly defecated pellets are not always available and environmental conditions, such as temperature and humidity, might affect faecal pellet consistency and FCM levels. Therefore, the impact of environmental conditions on FCMs needs to be evaluated. We collected 107 pellets from two female and two male koalas, exposed them to three types of treatments, and analysed FCMs in these samples with three enzyme immunoassays (EIAs). After analysis, the original FCM values were mathematically corrected for water loss. Results show that the FCMs were more stable when measured using tetrahydrocorticosterone (50c) and 5α-pregnane-3β,11β,21-triol-20-one (37e) EIAs, and were less stable when measured with the cortisol EIA. With 50c, the FCM values did not vary significantly over time either before or after the adjustment with water in the environment treatment group. For samples kept under constant low (25 °C) and high (35 °C) temperatures, the 50c FCM values did not vary significantly over time, after adjustments were made for water loss. Thus, this study highlights the importance of considering the suitability of faecal field samples for FCM analysis. Because water loss was the main driver of FCM changes, we strongly recommend collecting koala pellets that are freshly defecated, despite the effort and time it might take to collect such pellets.
Habitat Use and Activity Patterns of Mammals and Birds in Relation to Temperature and Vegetation Cover in the Alpine Ecosystem of Southwestern China with Camera-Trapping Monitoring
Authors: Li Zhouyuan , Tang Zhuo , Xu Yanjie , Wang Yingying , Duan Zhaogang , Liu Xuehua , Wang Pengyan , Yang Jian , Chen Wei , Prins Herbert H. T.
Published: November 2021

The high-altitude ecosystem of the Tibetan Plateau in China is a biodiversity hotspot that provides unique habitats for endemic and relict species along an altitudinal gradient at the eastern edge. Acquiring biodiversity information in this area, where the average altitude is over 4000 m, has been difficult but has been aided by recent developments in non-invasive technology, including infrared-triggered camera trapping. We used camera trapping to acquire a substantial number of photographic wildlife records in Wolong National Nature Reserve, Sichuan, China, from 2013 to 2016. We collected information of the habitat surrounding the observation sites, resulting in a dataset covering 37 species and 12 environmental factors. We performed a multivariate statistical analysis to discern the dominant environmental factors and cluster the mammals and birds of the ecosystem in order to examine environmental factors contributing to the species’ relative abundance. Species were generalized into three main types, i.e., cold-resistant, phyllophilic, and thermophilic, according to the identified key environmental drivers (i.e., temperature and vegetation) for their abundances. The mammal species with the highest relative abundance were bharal (Pseudois nayaur), Moupin pika (Ochotona thibetana), and Himalayan marmot (Marmota himalayana). The bird species with highest relative abundance were snow partridge (Lerwa lerwa), plain mountain finch (Leucosticte nemoricola), Chinese monal (Lophophorus lhuysii), and alpine accentor (Prunella collaris).
An Update on Cephenemyiosis in the European Roe Deer: Emergent Myiasis in Spain
Authors: Morrondo Patrocinio , Pajares Gerardo , Arias María Sol , Martínez-Calabuig Néstor , Remesar Susana , García-Dios David , Díaz Pablo , López Ceferino Manuel , Panadero Rosario , Díez-Baños Pablo
Published: November 2021

Cephenemyia stimulator is a Palearctic species developing in the nasal cavity and pharynx of roe deer (Capreolus capreolus). It is widely spread in the range of distribution of this ungulate in Europe. Since the first report of C. stimulator in Spain in 2001, a rapid geographic expansion has been observed, first in the north of the country, with high prevalence and intensities of infestation that caused some mortal cases, and, lately, also in Extremadura and Andalucía, the southernmost populations of European roe deer. These observations suggest an adaptation of this parasite to different ecosystems of the Iberian Peninsula. Almost simultaneously, C. stimulator is also expanding its range to northern Europe, with the first cases being reported in Sweden. Thus, Cephenemyia stimulator may be an example of a parasite currently displaying distributional changes along its southernmost and northernmost range margins. Thus, it is of the utmost importance to unravel all the epidemiological and clinical aspects of this myiasis, as well as implementing surveillance measures including reliable and non-invasive diagnostic techniques to monitor its expansion and adaptation to different ecosystems and/or hosts and to reduce the negative impact on roe deer populations.
Age at First Gestation in Beef Heifers Affects Fetal and Postnatal Growth, Glucose Metabolism and IGF1 Concentration
Authors: López Valiente Sebastian , Rodríguez Alejandro M. , Long Nathan M. , Quintans Graciela , Miccoli Florencia E. , Lacau-Mengido Isabel M. , Maresca Sebastian
Published: November 2021

This study aimed to determine the effects of age at first gestation on offspring growth performance, glucose metabolism, and IGF1 concentration. Heifers impregnated by AI from a single bull at 15 months of age (15 M, n = 20), or 27 months of age (27 M, n = 20), and multiparous cows (adult, n = 20) were used. Dams from all groups were managed in a single group during gestation and lactation. Gestational length was longer in the 15 M and 27 M than in adult dams (p = 0.009). Bodyweight at birth, at weaning and ADG during lactation were higher in calves from adult dams than in those from 27 M dams, and higher in calves from the latter than in 15 M calves (p < 0.001). Calves from 15 M dams had an increased head circumference/BW ratio compared to calves from 27 M dams, while calves from this latter group had an increased ratio compared to calves from adults (p = 0.005). Body mass index was greater in calves from adults than in those from 15 M and 27 M dams (p = 0.002). Milk production from 15 M and 27 M dams was similar but lower than that from adults (p = 0.03). Calves born from adult dams had greater blood glucose concentrations than those from 15 M and 27 M dams (p < 0.05). Serum IGF1 concentrations were higher in calves from adults than in calves from 15 M and 27 M dams (p = 0.01). This study showed that age at first gestation affects offspring postnatal growth performance, glucose metabolism and IGF1 concentration.
Host Identity and Geographic Location Significantly Affect Gastrointestinal Microbial Richness and Diversity in Western Lowland Gorillas (Gorilla gorilla gorilla) under Human Care
Authors: Eschweiler Katrina , Clayton Jonathan B. , Moresco Anneke , McKenney Erin A. , Minter Larry J. , Suhr Van Haute Mallory J. , Gasper William , Hayer Shivdeep Singh , Zhu Lifeng , Cooper Kathryn , Ange-van Heugten Kimberly
Published: November 2021

The last few decades have seen an outpouring of gastrointestinal (GI) microbiome studies across diverse host species. Studies have ranged from assessments of GI microbial richness and diversity to classification of novel microbial lineages. Assessments of the “normal” state of the GI microbiome composition across multiple host species has gained increasing importance for distinguishing healthy versus diseased states. This study aimed to determine baselines and trends over time to establish “typical” patterns of GI microbial richness and diversity, as well as inter-individual variation, in three populations of western lowland gorillas (Gorilla gorilla gorilla) under human care at three zoological institutions in North America. Fecal samples were collected from 19 western lowland gorillas every two weeks for seven months (n = 248). Host identity and host institution significantly affected GI microbiome community composition (p < 0.05), although host identity had the most consistent and significant effect on richness (p = 0.03) and Shannon diversity (p = 0.004) across institutions. Significant changes in microbial abundance over time were observed only at Denver Zoo (p < 0.05). Our results suggest that individuality contributes to most of the observed GI microbiome variation in the study populations. Our results also showed no significant changes in any individual’s microbial richness or Shannon diversity during the 7-month study period. While some microbial taxa (Prevotella, Prevotellaceae and Ruminococcaceae) were detected in all gorillas at varying levels, determining individual baselines for microbial composition comparisons may be the most useful diagnostic tool for optimizing non-human primate health under human care.
NEFA Promotes Autophagosome Formation through Modulating PERK Signaling Pathway in Bovine Hepatocytes
Authors: Huang Yan , Zhao Chenxu , Liu Yaoquan , Kong Yezi , Tan Panpan , Liu Siqi , Zeng Fangyuan , Yuan Yang , Li Xinwei , Liu Guowen , Zhao Baoyu , Wang Jianguo
Published: November 2021

During the perinatal period, the abnormally high plasma non-esterified fatty acids (NEFA) concentration caused by the negative energy balance (NEB) can impose a significant metabolic stress on the liver of dairy cows. Endoplasmic reticulum (ER) stress is an important adaptive response that can serve to maintain cell homeostasis in the event of stress. The protein kinase R-like endoplasmic reticulum kinase (PERK) pathway is the most rapidly activated cascade when ER stress occurs in cells and has an important impact on the regulation of hepatic lipid metabolism and autophagy modulation. However, it is unknown whether NEFA can affect autophagy through modulating the PERK pathway, under NEB conditions. In this study, we provide evidence that NEFA treatment markedly increased lipid accumulation, the phosphorylation level of PERK and eukaryotic initiation factor 2α (eIF2α), and the expression of glucose-regulated protein 78 (Grp78), activating transcription factor 4 (ATF4), and C/EBP homologous protein (CHOP). More importantly, NEFA treatment can cause a substantial increase in the protein levels of autophagy-related gene 7 (ATG7), Beclin-1 (BECN1), sequestosome-1 (p62), and microtubule-associated protein 1 light chain 3 (LC3)-II, and in the number of autophagosomes in primary bovine hepatocytes. The addition of GSK2656157 (PERK phosphorylation inhibitor) can significantly inhibit the effect of NEFA on autophagy and can further increase lipid accumulation. Overall, our results indicate that NEFA could promote autophagy via the PERK pathway in bovine hepatocytes. These findings provide novel evidence about the potential role of the PERK signaling pathway in maintaining bovine hepatocyte homeostasis.
Prevalence and Antimicrobial Resistance Profiles of Foodborne Pathogens Isolated from Dairy Cattle and Poultry Manure Amended Farms in Northeastern Ohio, the United States
Authors: Hailu Woinshet , Helmy Yosra A. , Carney-Knisely Geoffrey , Kauffman Michael , Fraga Dean , Rajashekara Gireesh
Published: November 2021

Foodborne pathogens significantly impact public health globally. Excessive antimicrobial use plays a significant role in the development of the public health crisis of antibiotic resistance. Here, we determined the prevalence and antimicrobial resistance profiles of E. coli O157, Salmonella, L. monocytogenes, and Campylobacter isolated between 2016 and 2020 from small scale agricultural settings that were amended with dairy cattle or poultry manure in Northeastern Ohio. The total prevalence of the foodborne pathogens was 19.3%: Campylobacter 8%, Listeria monocytogenes 7.9%, Escherichia coli O157 1.8%, and Salmonella 1.5%. The prevalence was significantly higher in dairy cattle (87.7%) compared to poultry (12.2%) manure amended farms. Furthermore, the prevalence was higher in manure samples (84%) compared to soil samples (15.9%; p < 0.05). Multiple drug resistance was observed in 73%, 77%, 100%, and 57.3% of E. coli O157, Salmonella, L. monocytogenes, and Campylobacter isolates recovered, respectively. The most frequently observed resistance genes were mphA, aadA, and aphA1 in E. coli O157; blaTEM, tet(B), and strA in Salmonella; penA, ampC, lde, ermB, tet(O), and aadB in L. monocytogenes and blaOXA-61, tet(O), and aadE in Campylobacter. Our results highlight the critical need to address the dissemination of foodborne pathogens and antibiotic resistance in agricultural settings.
Personalized Antibiotic Therapy for the Critically Ill: Implementation Strategies and Effects on Clinical Outcome of Piperacillin Therapeutic Drug Monitoring—A Descriptive Retrospective Analysis
Authors: Nikolas Schrader , Thorsten Riese , Max Kurlbaum , Patrick Meybohm , Markus Kredel , Güzin Surat , Oliver Scherf-Clavel , Alexander Strate , Andreas Pospiech , Kerstin Hoppe
Published: November 2021

Therapeutic drug monitoring (TDM) is increasingly relevant for an individualized antibiotic therapy and subsequently a necessary tool to reduce multidrug-resistant pathogens, especially in light of diminishing antimicrobial capabilities. Critical illness is associated with profound pharmacokinetic and pharmacodynamic alterations, which challenge dose finding and the application of particularly hydrophilic drugs such as β-lactam antibiotics. Methods: Implementation strategy, potential benefit, and practicability of the developed standard operating procedures were retrospectively analyzed from January to December 2020. Furthermore, the efficacy of the proposed dosing target of piperacillin in critically ill patients was evaluated. Results: In total, 160 patients received piperacillin/tazobactam therapy and were subsequently included in the study. Of them, 114 patients received piperacillin/tazobactam by continuous infusion and had at least one measurement of piperacillin serum level according to the standard operating procedure. In total, 271 measurements were performed with an average level of 79.0 ± 46.0 mg/L. Seventy-one piperacillin levels exceeded 100 mg/L and six levels were lower than 22.5 mg/L. The high-level and the low-level group differed significantly in infection laboratory parameters (CRP (mg/dL) 20.18 ± 11.71 vs. 5.75 ± 5.33) and renal function [glomerular filtration rate (mL/min/1.75 m2) 40.85 ± 26.74 vs. 120.50 ± 70.48]. Conclusions: Piperacillin levels are unpredictable in critically ill patients. TDM during piperacillin/tazobactam therapy is highly recommended for all patients. Although our implementation strategy was effective, further strategies implemented into the daily clinical workflow might support the health care staff and increase the clinicians’ alertness.
Dose Optimization of Colistin: A Systematic Review
Authors: Haseeb Abdul , Faidah Hani Saleh , Alghamdi Saleh , Alotaibi Amal F. , Elrggal Mahmoud Essam , Mahrous Ahmad Jamal , Almarzoky Abuhussain Safa S. , Obaid Najla A. , Algethamy Manal , AlQarni Abdullmoin , Khogeer Asim A. , Saleem Zikria , Sheikh Aziz
Published: November 2021

Colistin is considered a last treatment option for multi-drug and extensively resistant Gram-negative infections. We aimed to assess the available data on the dosing strategy of colistin. A systematic review was performed to identify all published studies on the dose optimization of colistin. Grey literature and electronic databases were searched. Data were collected in a specified form and the quality of the included articles was then assessed using the Newcastle-Ottawa scale for cohort studies, the Cochrane bias tool for randomized clinical trials (RCT), and the Joanna Briggs Institute (JBI) critical checklist for case reports. A total of 19 studies were included, of which 16 were cohort studies, one was a RCT, and two were case reports. A total of 18 studies proposed a dosing regimen for adults, while only one study proposed a dosing schedule for pediatric populations. As per the available evidence, a loading dose of 9 million international units (MIU) of colistin followed by a maintenance dose of 4.5 MIU every 12 h was considered the most appropriate dosing strategy to optimize the safety and efficacy of treatment and improve clinical outcomes. This review supports the administration of a loading dose followed by a maintenance dose of colistin in severe and life-threatening multi-drug Gram-negative bacterial infections.
Mutational Diversity in the Quinolone Resistance-Determining Regions of Type-II Topoisomerases of Salmonella Serovars
Authors: Shaheen Aqsa , Tariq Anam , Iqbal Mazhar , Mirza Osman , Haque Abdul , Walz Thomas , Rahman Moazur
Published: November 2021

Quinolone resistance in bacterial pathogens has primarily been associated with mutations in the quinolone resistance-determining regions (QRDRs) of bacterial type-II topoisomerases, which are DNA gyrase and topoisomerase IV. Depending on the position and type of the mutation (s) in the QRDRs, bacteria either become partially or completely resistant to quinolone. QRDR mutations have been identified and characterized in Salmonella enterica isolates from around the globe, particularly during the last decade, and efforts have been made to understand the propensity of different serovars to carry such mutations. Because there is currently no thorough analysis of the available literature on QRDR mutations in different Salmonella serovars, this review aims to provide a comprehensive picture of the mutational diversity in QRDRs of Salmonella serovars, summarizing the literature related to both typhoidal and non-typhoidal Salmonella serovars with a special emphasis on recent findings. This review will also discuss plasmid-mediated quinolone-resistance determinants with respect to their additive or synergistic contributions with QRDR mutations in imparting elevated quinolone resistance. Finally, the review will assess the contribution of membrane transporter-mediated quinolone efflux to quinolone resistance in strains carrying QRDR mutations. This information should be helpful to guide the routine surveillance of foodborne Salmonella serovars, especially with respect to their spread across countries, as well as to improve laboratory diagnosis of quinolone-resistant Salmonella strains.
The Herbal Formula JI017 Induces ER Stress via Nox4 in Breast Cancer Cells
Authors: Kim Tae Woo , Ko Seong-Gyu
Published: November 2021

Chemotherapy is a powerful anti-tumor therapeutic strategy; however, resistance to treatment remains a serious concern. To overcome chemoresistance, combination therapy with anticancer drugs is a potential strategy. We developed a novel herbal extract, JI017, with lower toxicity and lesser side effects. JI017 induced programmed cell death and excessive unfolded protein response through the release of intracellular reactive oxygen species (ROS) and calcium in breast cancer cells. JI017 treatment increased the expression of endoplasmic reticulum (ER) stress markers, including p-PERK, p-eIF2α, ATF4, and CHOP, via the activation of both exosomal GRP78 and cell lysate GRP78. The ROS inhibitors diphenyleneiodonium and N-acetyl cysteine suppressed apoptosis and excessive ER stress by inhibiting Nox4 in JI017-treated breast cancer cells. Furthermore, in paclitaxel-resistant breast cancer cell lines, MCF-7R and MDA-MB-231R, a combination of JI017 and paclitaxel overcame paclitaxel resistance by blocking epithelial-mesenchymal transition (EMT) processes, such as the downregulation of E-cadherin expression and the upregulation of HIF-1α, vimentin, Snail, and Slug expression. These findings suggested that JI017 exerts a powerful anti-cancer effect in breast cancer and a combination therapy of JI017 and paclitaxel may be a potential cancer therapy for paclitaxel resistant breast cancer.
Effects of Folinic Acid Administration on Lower Limb Ischemia/Reperfusion Injury in Rats
Authors: Cearra Iñigo , Herrero de la Parte Borja , Ruiz Montesinos Inmaculada , Alonso-Varona Ana , Moreno-Franco Diana Isabel , García-Alonso Ignacio
Published: November 2021

Surgery under ischemic conditions, lasting up to 3 h, is routinely performed in orthopedic surgery, causing undesirable injury due to ischemia-reperfusion syndrome, with short and medium-term functional repercussions. To date, there is no established prophylactic treatment. In this work we evaluated folinic acid (FA) in a rodent model of lower limb ischemia-reperfusion (IRI-LL). 36 male WAG rats underwent 3 h of lower limb ischemia. In the saline group, rats received intraperitoneal administration of saline (used as vehicle for treatment). In the experimental group, rats were pretreated with FA (2.5 mg/kg) before the end of ischemia. After ischemia, animals were sacrificed at 3 h, 24 h or 14 days (for biochemical determination (Na+, K+, Cl-, urea, creatinine, CK, LDH, ALP, ALT, and AST), pathological assessment, or functional study using the rotarod test; respectively). Another six animals were used to establish the reference values. The prophylactic administration of FA significantly reduced the elevation of biochemical markers, especially those that most directly indicate muscle damage (CK and LDH). In addition, it also improved direct tissue damage, both in terms of edema, weight, PMN infiltrate and percentage of damaged fibers. Finally, the administration of FA allowed the animals to equal baseline values in the rotarod test; what did not occur in the saline group, where pre-ischemia levels were not recovered. Following 3 h of lower limb ischemia, FA minimizes the increase of CK and LDH, as well as local edema and leukocyte infiltration, allowing a faster recovery of limb functionality. Therefore, it could be considered as a prophylactic treatment when tourniquet is used in clinics.
An Overview on the Therapeutic Function of Foods Enriched with Plant Sterols in Diabetes Management
Authors: Jayaraman Selvaraj , Roy Anitha , Vengadassalapathy Srinivasan , Sekar Ramya , Veeraraghavan Vishnu Priya , Rajagopal Ponnulakshmi , Rengasamy Gayathri , Mukherjee Raktim , Sekar Durairaj , Manjunathan Reji
Published: November 2021

Diabetes is one of the most significant health issues across the world. People identified with diabetes are more vulnerable to various infections and are at a greater risk of developing cardiovascular diseases. The plant-based food we consume often contains many sterol-based bioactive compounds. It is well documented that these compounds could effectively manage the processes of insulin metabolism and cholesterol regulation. Insulin resistance followed by hyperglycemia often results in oxidative stress level enhancement and increased reactive oxygen species production. At the molecular level, these changes induce apoptosis in pancreatic cells and hence lead to insulin insufficiency. Studies have proved that plant sterols can lower inflammatory and oxidative stress damage connected with DNA repair mechanisms. The effective forms of phyto compounds are polyphenols, terpenoids, and thiols abundant in vegetables, fruits, nuts, and seeds. The available conventional drug-based therapies for the prevention and management of diabetes are time-consuming, costly, and with life-threatening side effects. Thereby, the therapeutic management of diabetes with plant sterols available in our daily diet is highly welcome as there are no side effects. This review intends to offer an overview of the present scenario of the anti-diabetic compounds from food ingredients towards the therapeutic beneficial against diabetes.
Experimental Study on the Effects of In Situ Stress on the Initiation and Propagation of Cracks during Hard Rock Blasting
Authors: Yan Guangliang , Yang Qibo , Zhang Fengpeng , Hao Qiqi , Wang Xiulong , Wang Haonan
Published: November 2021

In situ stress is one of the most important factors affecting rock dynamic fractures during blasting excavation of deep rock mass that generally is hard rock. In this research, crater blasting experiments on hard rock under different uniaxial static stresses were conducted to investigate the initiation and propagation process of crack networks that were induced by coupled dynamic and static loads. Furthermore, the effects of anisotropic static stress fields on the initiation and propagation of crack networks during hard rock blasting, and the crack network morphological characteristics were analyzed and elucidated. The experimental results showed that the static stress field changed the process of crack network initiation and propagation during hard rock blasting, and then control the crack network morphology. Under uniaxial static stress, the crack network was elliptical with the long axis parallel to the static stress. In addition, the larger the anisotropic static stress is, the more obvious the elliptical morphology of the crack network. Moreover, the static stress lead to the delay of crack formation which indicates that the delay time during millisecond blasting excavation of deep rock mass should be adjusted appropriately according to the in situ stress. A stress-strength ratio (SSR) of 0.15 is the threshold value where static stress may have a significant effect on the initiation and propagation of a crack network. Meanwhile, the strain field prior to crack initiation during rock blasting controlled the morphological characteristics of the crack network. Finally, the mechanism of static stress affecting propagation and morphology of crack network was revealed theoretically.
Viewpoint Robustness of Automated Facial Action Unit Detection Systems
Authors: Namba Shushi , Sato Wataru , Yoshikawa Sakiko
Published: November 2021

Automatic facial action detection is important, but no previous studies have evaluated pre-trained models on the accuracy of facial action detection as the angle of the face changes from frontal to profile. Using static facial images obtained at various angles (0°, 15°, 30°, and 45°), we investigated the performance of three automated facial action detection systems (FaceReader, OpenFace, and Py-feat). The overall performance was best for OpenFace, followed by FaceReader and Py-Feat. The performance of FaceReader significantly decreased at 45° compared to that at other angles, while the performance of Py-Feat did not differ among the four angles. The performance of OpenFace decreased as the target face turned sideways. Prediction accuracy and robustness to angle changes varied with the target facial components and action detection system.
A Durable PVDF/PFOTES-SiO2 Superhydrophobic Coating on AZ31B Mg Alloy with Enhanced Abrasion Resistance Performance and Anti-Corrosion Properties
Authors: Qian Zhiqiang , Liu Zhong , Wang Shidong , Ye XiuShen , Wu Zhijian
Published: November 2021

A simple and practical spray method is employed to prepare a PVDF/PFOTES-SiO2 superhydrophobic composite coating on the AZ31B Mg alloy substrate. The morphology, composition, and water contact angle (CA) were measured by Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscope (FESEM) and contact angle measuring instrument. Hydrophilic nano-SiO2 is modified by PFOTES to obtain hydrophobicity. The influence of the mass of PFOTES-SiO2 to PVDF on the hydrophobic properties was studied. The wear resistance and stability of the composite coating have been investigated by immersion test, cross-cut adhesion test and friction test. Additionally, the corrosion resistance was measured by electrochemical workstation and salt spray corrosion test. The CA of PVDF/PFOTES-SiO2 coating is 161.3° and the sliding angle (SAs) is less than 2°. After 10× the sandpaper friction test, the superhydrophobic contact angle of the coating remained above 155°, and the sliding angle was less than 5°, which indicated that the prepared coating is a strong superhydrophobic coating with good wear resistance. The results of the electrochemical tests show that the superhydrophobic coating improved the anti-corrosion performance of Mg alloy, and the water contact angle is greater than 150° after 168 h salt spray corrosion test. Due to its excellent superhydrophobicity, wear resistance and anti-corrosion properties, the robust PVDF/PFOTES-SiO2 coating is considered to have great potential for future applications in the automotive and marine industries.
Characterization of an X-ray Source Generated by a Portable Low-Current X-Pinch
Authors: Skoulakis Alexandros , Kaselouris Evaggelos , Kavroulakis Antonis , Karvounis Christos , Fitilis Ioannis , Chatzakis John , Dimitriou Vasilis , Papadogiannis Nektarios A. , Tatarakis Michael
Published: November 2021

An X-pinch scheme of a low-current generator (45 kA, 50 ns rise time) is characterized as a potential efficient source of soft X-rays. The X-pinch target consists of wires of 5 μm in diameter—made from either tungsten (W) or gold (Au)-plated W—loaded at two angles of 55° and 98° between the crossed wires. Time-resolved soft X-ray emission measurements are performed to provide a secure correlation with the optical probing results. A reconstruction of the actual photodiode current profile procedure was adopted, capable of overcoming the limits of the slow rising and falling times due to the “slow” response of the diodes and the noise. The pure and Au-plated W deliver an average X-ray yield, which depends only on the angle of the crossed wires, and is measured to be ~50 mJ and ~70 mJ for the 98° and 55° crossed wire angles, respectively. An additional experimental setup was developed to characterize the X-pinch as a source of X-rays with energy higher than ~6 keV, via time-integrated measurements. The X-ray emission spectrum was found to have an upper limit at 13 keV for the Au-plated W configuration at 55°. The portable tabletop X-pinch proved to be ideal for use in X-ray radiography applications, such as the detection of interior defects in biological samples.
Towards Robust Object Detection in Floor Plan Images: A Data Augmentation Approach
Authors: Mishra Shashank , Hashmi Khurram Azeem , Pagani Alain , Liwicki Marcus , Stricker Didier , Afzal Muhammad Zeshan
Published: November 2021

Object detection is one of the most critical tasks in the field of Computer vision. This task comprises identifying and localizing an object in the image. Architectural floor plans represent the layout of buildings and apartments. The floor plans consist of walls, windows, stairs, and other furniture objects. While recognizing floor plan objects is straightforward for humans, automatically processing floor plans and recognizing objects is challenging. In this work, we investigate the performance of the recently introduced Cascade Mask R-CNN network to solve object detection in floor plan images. Furthermore, we experimentally establish that deformable convolution works better than conventional convolutions in the proposed framework. Prior datasets for object detection in floor plan images are either publicly unavailable or contain few samples. We introduce SFPI, a novel synthetic floor plan dataset consisting of 10,000 images to address this issue. Our proposed method conveniently exceeds the previous state-of-the-art results on the SESYD dataset with an mAP of 98.1%. Moreover, it sets impressive baseline results on our novel SFPI dataset with an mAP of 99.8%. We believe that introducing the modern dataset enables the researcher to enhance the research in this domain.
Methylmercury and Polycyclic Aromatic Hydrocarbons in Mediterranean Seafood: A Molecular Anthropological Perspective
Authors: De Giovanni Andrea , Giuliani Cristina , Marini Mauro , Luiselli Donata
Published: November 2021

Eating seafood has numerous health benefits; however, it constitutes one of the main sources of exposure to several harmful environmental pollutants, both of anthropogenic and natural origin. Among these, methylmercury and polycyclic aromatic hydrocarbons give rise to concerns related to their possible effects on human biology. In the present review, we summarize the results of epidemiological investigations on the genetic component of individual susceptibility to methylmercury and polycyclic aromatic hydrocarbons exposure in humans, and on the effects that these two pollutants have on human epigenetic profiles (DNA methylation). Then, we provide evidence that Mediterranean coastal communities represent an informative case study to investigate the potential impact of methylmercury and polycyclic aromatic hydrocarbons on the human genome and epigenome, since they are characterized by a traditionally high local seafood consumption, and given the characteristics that render the Mediterranean Sea particularly polluted. Finally, we discuss the challenges of a molecular anthropological approach to this topic.
Perspective Compounds for Immobilization of Spent Electrolyte from Pyrochemical Processing of Spent Nuclear Fuel
Authors: Kulikova Svetlana A. , Danilov Sergey S. , Matveenko Anna V. , Frolova Anna V. , Belova Kseniya Y. , Petrov Vladimir G. , Vinokurov Sergey E. , Myasoedov Boris F.
Published: November 2021

Immobilization of spent electrolyte–radioactive waste (RW) generated during the pyrochemical processing of mixed nitride uranium–plutonium spent nuclear fuel is an acute task for further development of the closed nuclear fuel cycle with fast neutron reactors. The electrolyte is a mixture of chloride salts that cannot be immobilized directly in conventional cement or glass matrix. In this work, a low-temperature magnesium potassium phosphate (MPP) matrix and two types of high-temperature matrices (sodium aluminoironphosphate (NAFP) glass and ceramics based on bentonite clay) were synthesized. Two systems (Li0.4K0.28La0.08Cs0.016Sr0.016Ba0.016Cl and Li0.56K0.40Cs0.02Sr0.02Cl) were used as spent electrolyte imitators. The phase composition and structure of obtained materials were studied by XRD and SEM-EDS methods. The differential leaching rate of Cs from MPP compound and ceramic based on bentonite clay was about 10−5 g/(cm2·day), and the rate of Na from NAFP glass was about 10−6 g/(cm2·day). The rate of 239Pu from MPP compound (leaching at 25 °C) and NAFP glass (leaching at 90 °C) was about 10−6 and 10−7 g/(cm2·day), respectively. All the synthesized materials demonstrated high hydrolytic, mechanical compression strength (40–50 MPa) even after thermal (up to 450 °C) and irradiation (up to 109 Gy) tests. The characteristics of the studied matrices correspond to the current requirements to immobilized high-level RW, that allow us to suggest these materials for industrial processing of the spent electrolyte.
Application in Nanomedicine of Manganese-Zinc Ferrite Nanoparticles
Authors: Slavu Laura Maria , Rinaldi Rosaria , Di Corato Riccardo
Published: November 2021

Besides the study and the medical application of iron oxide nanoparticles, ferrites produced with zinc and manganese are of particular interest for their properties. The introduction of these elements into the crystalline structure of the magnetic particle generates some changes in the material properties, enhancing their potential use in theranostic applications. This review will cover the most important aspects of the preparation of these materials, taking into account the different methods of synthesis, and will analyze the most promising results in their use in MRI, magnetic hyperthermia and other emerging applications.
Deep Transfer Learning Approach for Identifying Slope Surface Cracks
Authors: Yang Yuting , Mei Gang
Published: November 2021

Geohazards such as landslides, which are often accompanied by surface cracks, have caused great harm to public safety and property. If these surface cracks could be identified in time, this would be of great significance for the monitoring and early warning of geohazards. Currently, the most common method for crack identification is manual detection, which has low efficiency and accuracy. In this paper, a deep transfer learning approach is proposed to effectively and efficiently identify slope surface cracks for the sake of fast monitoring and early warning of geohazards, such as landslides. The essential idea is to employ transfer learning by training (a) a large sample dataset of concrete cracks and (b) a small sample dataset of soil and rock masses’ cracks. In the proposed approach, (1) pretrained crack identification models are constructed based on a large sample dataset of concrete cracks; (2) refined crack identification models are further constructed based on a small sample dataset of soil and rock masses’ cracks. The proposed approach could be applied to conduct UAV surveys on high and steep slopes to provide monitoring and early warning of landslides to ensure the safety of people and property.
Advanced Electron Beam (EB) Wastewater Treatment System with Low Background X-ray Intensity Generation
Authors: Gryczka Urszula , Zimek Zbigniew , Walo Marta , Chmielewska-Śmietanko Dagmara , Bułka Sylwester
Published: November 2021

Electron beam wastewater treatment is a very effective method for the destruction of organic and microbiological pollutants. The technology was implemented for municipal and textile industry wastewater treatment. Availability of electron accelerators characterized with different operation parameters make the technology applicable for different end-users and also for installation in confined spaces. In such a case, the design of wastewater irradiation room has to take into account the limited space available for shielding construction, which must restrict X-ray emission. Considering construction of an irradiation room for water treatment facility, it is important to focus not only on a stream formation for irradiation to achieve the desired electron penetration, but also on the reduction in x-ray generation. In the presented work, the X-ray field was tested, using modelling and experimental methods. The final results gave an advanced solution, which can be used in the installation of wastewater treatment, ballast and other types of origin, providing low cost shield and good radiation protection measures.