bluuebunny/crossref_metadata_2025 huggingface dataset:
Deep Sequencing MicroRNAs from Extracellular Membrane Vesicles Revealed the Association of the Vesicle Cargo with Cellular Origin
Authors: Than Uyen Thi Trang , Guanzon Dominic , Broadbent James A , Parker Tony J , Leavesley David I
Published: February 2020

Extracellular membrane vesicles (EVs) have emerged as potential candidates for diagnostics and therapeutics. We have previously reported that keratinocytes release three types of EVs into the extracellular environment. Importantly, those EVs contain a large number of microRNAs (miRNAs) as cargo. In this study, we examined the expression level of keratinocyte-derived EV miRNAs, their target genes and potential functions. Next generation sequencing results showed that over one hundred miRNAs in each EV subtype exhibited greater than 100 reads per million (RPM), indicating a relatively high abundance. Analysis of the miRNAs with the highest abundance revealed associations with different keratinocyte cell sources. For instance, hsa-miR-205 was associated with the HaCaT cells whereas hsa-miR-21, hsa-miR-203, hsa-miR-22 and hsa-miR-143 were associated with human primary dermal keratinocytes (PKCs). Additionally, functional annotation analysis of genes regulated by those miRNAs, especially with regard to biological processes, also revealed cell-type-specific associations with either HaCaTs or PKCs. Indeed, EV functional effects were related to their parental cellular origin; specifically, PKC-derived EVs influenced fibroblast migration whereas HaCaT-derived EVs did not. In addition, the data in this current study indicates that keratinocyte-derived EVs and/or their cargoes have potential applications for wound healing.
Stratifying Intraductal Papillary Mucinous Neoplasms by Cyst Fluid Analysis: Present and Future
Authors: Hao Scarlett , Takahashi Caitlin , Snyder Rebecca A. , Parikh Alexander A.
Published: February 2020

A significant proportion of patients with intraductal papillary mucinous neoplasms (IPMNs) undergo surgical resection in order to prevent or treat pancreatic cancer at the risk of significant perioperative morbidity. Efforts have been made to stratify the potential risk of malignancy based on the clinical and radiographic features of IPMN to delineate which cysts warrant resection versus observation. An analysis of the cyst fluid obtained by preoperative endoscopic examination appears to be correlative of cyst type and risk, whereas serum markers and radiographic findings have not yet reached a level of sensitivity or specificity that proves they are clinically meaningful. In this review, we investigate the current cyst fluid analysis studies and present those that have shown promise in effectively stratifying high-risk versus low-risk lesions. While new cyst fluid markers continue to be identified, additional efforts in testing panels and marker composites in conjunction with clinical algorithms have also shown promise in distinguishing dysplasia and the risk of malignancy. These should be tested prospectively in order to determine their role in guiding the surveillance of low-risk lesions and to evaluate the new markers detected by proteomics and genetic sequencing.
Genotoxic Properties of Synthetic Cannabinoids on TK6 Human Cells by Flow Cytometry
Authors: Lenzi Monia , Cocchi Veronica , Cavazza Luca , Bilel Sabrine , Hrelia Patrizia , Marti Matteo
Published: February 2020

Novel Psychoactive Substances (NPS) include several classes of substances such as synthetic cannabinoids (SCBs), an emerging alternative to marijuana, easily purchasable on internet. SCBs are more dangerous than Δ9-Tetrahydrocannabinol as a consequence of their stronger affinities for the CB1 and CB2 receptors, which may result in longer duration of distinct effects, greater potency, and toxicity. The information on SCBs cytotoxicity, genotoxicity, mutagenicity, and long-term effects is scarce. This fact suggests the urgent need to increase available data and to investigate if some SCBs have an impact on the stability of genetic material. Therefore, the aim of the present study was the evaluation of the mutagenic effect of different SCBs belonging to indole- and indazole-structures. The analyzes were conducted in vitro on human TK6 cells and mutagenicity were measured as micronucleus fold increase by flow cytometry. Our results have highlighted, for the first time, the mutagenic capacity of four SCBs, in particular in terms of chromosomal damage induction. We underline the serious potential toxicity of SCBs that suggests the need to proceed with the studies of other different synthetic compounds. Moreover, we identified a method that allows a rapid but effective screening of NPS placed on the market increasingly faster.
Testosterone Promotes the Proliferation of Chicken Embryonic Myoblasts Via Androgen Receptor Mediated PI3K/Akt Signaling Pathway
Authors: Li Dongfeng , Wang Qin , Shi Kai , Lu Yinglin , Yu Debing , Shi Xiaoli , Du Wenxing , Yu Minli
Published: February 2020

Testosterone (T) is essential for muscle fiber formation and growth. However, the specific mechanism by which T regulates skeletal muscle development in chicken embryos remains unclear. In this study, the role of T in myoblast proliferation both in vivo and in vitro was investigated. Results showed that the T administration significantly increased the ratio of breast muscle and leg muscle. T induced a significant increase in the cross-sectional area (CSA) and density of myofiber and the ratio of PAX7-positive cells in the skeletal muscle. Exogenous T also induced the upregulation of myogenic regulatory factors (MRFs) and cyclin-dependent kinases (CDK2)/Cyclin D1 (CCND1) and protein levels of androgen receptor (AR), p-Akt and PAX7. Furthermore, T treatment significantly promoted myoblasts cultured in vitro entering a new cell cycle and increased PAX7-positive cells. The mRNA and protein expression of AR and PAX7 were upregulated when treated with T compared to that of the control. The addition of T induced proliferation accompanied by increasing AR level as well as PI3K (Phosphoinositide 3-kinase)/Akt activation. However, T-induced proliferation was attenuated by AR, PI3K, and Akt-specific inhibitors. These data indicated that the pro-proliferative effect of T was regulated though AR in response to the activation of PI3K/Akt signalling pathway.
Aristolochic Acid-Induced Nephrotoxicity: Molecular Mechanisms and Potential Protective Approaches
Authors: Anger Etienne Empweb , Yu Feng , Li Ji
Published: February 2020

Aristolochic acid (AA) is a generic term that describes a group of structurally related compounds found in the Aristolochiaceae plants family. These plants have been used for decades to treat various diseases. However, the consumption of products derived from plants containing AA has been associated with the development of nephropathy and carcinoma, mainly the upper urothelial carcinoma (UUC). AA has been identified as the causative agent of these pathologies. Several studies on mechanisms of action of AA nephrotoxicity have been conducted, but the comprehensive mechanisms of AA-induced nephrotoxicity and carcinogenesis have not yet fully been elucidated, and therapeutic measures are therefore limited. This review aimed to summarize the molecular mechanisms underlying AA-induced nephrotoxicity with an emphasis on its enzymatic bioactivation, and to discuss some agents and their modes of action to reduce AA nephrotoxicity. By addressing these two aspects, including mechanisms of action of AA nephrotoxicity and protective approaches against the latter, and especially by covering the whole range of these protective agents, this review provides an overview on AA nephrotoxicity. It also reports new knowledge on mechanisms of AA-mediated nephrotoxicity recently published in the literature and provides suggestions for future studies.
Design and Application of a Case Analysis System for Handling Power Grid Operational Accidents Based on Case-Based Reasoning
Authors: Yu Xiaopeng , Xu Changqing , Lu Dan , Zhu Zeyu , Zhou Zhipeng , Ye Nan , Mi Chuanmin
Published: February 2020

In recent years, power grid accidents have occurred frequently and higher requirements have been placed on their safety operation. In current safety management in the world, there is an effective practice that uses a unified standard for structuring an accident case database and based on that database, conducts quantitative analysis to cope with accident risks. However, that is not the case for power safety management. Case-based reasoning (CBR) is such a process that solves new problems based on the solutions to similar past problems. It works by matching a current problem with historical cases and solutions in a database, in order to obtain similar case solutions or inspirations. In the matching process, if necessary, such past solutions may be modified in order to better adapt to the current actual problems. Based on the CBR method, this paper proposes how to construct a case database of power grid operational accidents, provide data support for management of power grid risks and provide knowledge services for accurate grasping of grid accident development dynamics and making quick decisions to rapidly response to the emergencies. First, it designs an operational accident case database after considering the following three aspects: case features, power grid features and accident features based on safety management theory. Secondly, in terms of how to use the power grid operational accident case database, it proposed a two-level search strategy, as well as the corresponding similarity calculation methods for different feature attributes of the case. Finally, it carried out a demonstration to verify the model by selecting four typical grid accidents. The grid database and CBR strategy proposed in this article could help China’s power grids practice intelligent analysis of grid operational accidents and improve digitalization in safety management.
A Novel Method for Twitter Sentiment Analysis Based on Attentional-Graph Neural Network
Authors: Wang Mingda , Hu Guangmin
Published: February 2020

Twitter sentiment analysis is an effective tool for various Twitter-based analysis tasks. However, there is still no neural-network-based research which takes both the tweet-text information and user-connection information into account. To this end, we propose the Attentional-graph Neural Network based Twitter Sentiment Analyzer (AGN-TSA), a Twitter sentiment analyzer based on attentional-graph neural networks. AGN-TSA fuses the tweet-text information and the user-connection information through a three-layered neural structure, which includes a word-embedding layer, a user-embedding layer and an attentional graph network layer. For the training of AGN-TSA, dedicated loss functions are designed for the structural controllability of AGN-TSA network. Experiments based on real-world dataset concerning the 2016 presidential election of America exhibit that AGN-TSA is superior under multiple metrics over several prevailing methods, with a performance boost of over 5%. The empirical settings of parameters are given based on extensive rotation experiments.
Saliva and Oral Diseases
Authors: Martina Emanuela , Campanati Anna , Diotallevi Federico , Offidani Annamaria
Published: February 2020

Saliva is a fascinating biological fluid which has all the features of a perfect diagnostic tool. In fact, its collection is rapid, simple, and noninvasive. Thanks to several transport mechanisms and its intimate contact with crevicular fluid, saliva contains hundreds of proteins deriving from plasma. Advances in analytical techniques have opened a new era—called “salivaomics”—that investigates the salivary proteome, transcriptome, microRNAs, metabolome, and microbiome. In recent years, researchers have tried to find salivary biomarkers for oral and systemic diseases with various protocols and technologies. The review aspires to provide an overall perspective of salivary biomarkers concerning oral diseases such as lichen planus, oral cancer, blistering diseases, and psoriasis. Saliva has proved to be a promising substrate for the early detection of oral diseases and the evaluation of therapeutic response. However, the wide variation in sampling, processing, and measuring of salivary elements still represents a limit for the application in clinical practice.
Regulation of Oligodendrocyte Functions: Targeting Lipid Metabolism and Extracellular Matrix for Myelin Repair
Authors: Marangon Davide , Boccazzi Marta , Lecca Davide , Fumagalli Marta
Published: February 2020

Myelin is an essential structure that protects axons, provides metabolic support to neurons and allows fast nerve transmission. Several neurological diseases, such as multiple sclerosis, are characterized by myelin damage, which is responsible of severe functional impairment. Myelin repair requires the timely recruitment of adult oligodendrocyte precursor cells (OPCs) at the lesion sites, their differentiation and maturation into myelinating oligodendrocytes. As a consequence, OPCs undergo profound changes in their morphology, functions, and interactions with other cells and extracellular environment, thus requiring the reorganization of both their lipid metabolism and their membrane composition, which is substantially different compared to other plasma membranes. Despite the growing knowledge in oligodendroglia biology and in the mechanisms involved in OPC-mediated regeneration, the identification of strategies to promote remyelination still remains a challenge. Here, we describe how altered lipid metabolism in oligodendrocytes influences the pathogenesis of demyelination, and we show that several FDA-approved drugs with a previously unknown remyelination potential do act on cholesterol and lipid biosynthetic pathways. Since the interplay between myelin lipids and axons is strictly coordinated by the extracellular matrix (ECM), we also discuss the role of different ECM components, and report the last findings on new ECM-modifiers able to foster endogenous remyelination.
Mechanisms of Testicular Disruption from Exposure to Bisphenol A and Phtalates
Authors: Pallotti Francesco , Pelloni Marianna , Gianfrilli Daniele , Lenzi Andrea , Lombardo Francesco , Paoli Donatella
Published: February 2020

Great attention has been paid in recent years to the harmful effects of various chemicals that interfere with our natural hormone balance, collectively known as endocrine-disrupting chemicals (EDCs) or endocrine disruptors. The effects on the reproductive system of bisphenol A (BPA) and phthalates have received particular attention: while they have a short half-life, they are so widespread that human exposure can be considered as continuous. Evidence is often limited to the animal model, disregarding the likelihood of human exposure to a mixture of contaminants. Data from animal models show that maternal exposure probably has harmful effects on the male fetus, with an increased risk of urogenital developmental abnormalities. After birth, exposure is associated with changes in the hypothalamic-pituitary-testicular axis, hindering the development and function of the male genital pathways through the mediation of inflammatory mechanisms and oxidative stress. The epidemiological and clinical evidence, while generally confirming the association between reproductive abnormalities and some phthalate esters and BPA, is more contradictory, with wildly different findings. The aim of this review is therefore to provide an update of the potential mechanisms of the damage caused by BPA and phthalates to reproductive function and a review of the clinical evidence currently available in the literature.
Electrochemical Disinfection of Dental Implants Experimentally Contaminated with Microorganisms as a Model for Periimplantitis
Authors: Koch Maximilian , Göltz Maximilian , Xiangjun Meng , Karl Matthias , Rosiwal Stefan , Burkovski Andreas
Published: February 2020

Despite several methods having been described for disinfecting implants affected by periimplantitis, none of these are universally effective and may even alter surfaces and mechanical properties of implants. Boron-doped diamond (BDD) electrodes were fabricated from niobium wires and assembled as a single instrument for implant cleaning. Chemo-mechanical debridement and air abrasion were used as control methods. Different mono-species biofilms, formed by bacteria and yeasts, were allowed to develop in rich medium at 37 °C for three days. In addition, natural multi-species biofilms were treated. Implants were placed in silicone, polyurethane foam and bovine ribs for simulating different clinical conditions. Following treatment, the implants were rolled on blood agar plates, which were subsequently incubated at 37 °C and microbial growth was analyzed. Complete electrochemical disinfection of implant surfaces was achieved with a maximum treatment time of 20 min for Candida albicans, Candida dubliniensis, Enterococcus faecalis, Roseomonas mucosa, Staphylococcus epidermidis and Streptococcus sanguinis, while in case of spore-forming Bacillus pumilus and Bacillus subtilis, a number of colonies appeared after BDD electrode treatment indicating an incomplete disinfection. Independent of the species tested, complete disinfection was never achieved when conventional techniques were used. During treatment with BDD electrodes, only minor changes in temperature and pH value were observed. The instrument used here requires optimization so that higher charge quantities can be applied in shorter treatment times.
Pilot Study of Aerosolised Plus Intravenous Vancomycin in Mechanically Ventilated Patients with Methicillin-Resistant Staphylococcus Aureus Pneumonia
Authors: Cho Jun Yeun , Kim Hyung-Sook , Yang Hye-Joo , Lee Yeon Joo , Park Jong Sun , Yoon Ho Il , Kim Hong Bin , Yim Jae-Joon , Lee Jae-Ho , Lee Choon-Taek , Cho Young-Jae
Published: February 2020

Treatment of methicillin-resistant Staphylococcus aureus (MRSA) pneumonia in critically ill patients remains unsatisfactory. This pilot study aimed to evaluate the clinical outcomes of aerosolised vancomycin in addition to intravenous administration in this setting. This was a prospective, noncomparative, phase II trial. Patients receiving mechanical ventilation for >48 h in intensive care units (ICUs) were screened; those receiving intravenous vancomycin for MRSA pneumonia were enrolled. Patients received aerosolised vancomycin (250 mg every 12 h for five days) via a vibrating mesh nebuliser. The primary outcome was treatment success (clinical cure or improvement) at the conclusion of antibiotic treatment. Vancomycin concentrations were measured in bronchoalveolar lavage fluid according to administration time. Twenty patients were enrolled (median age 75 years and 13 (65%) men; 18 (90%) cases with nosocomial pneumonia). Thirteen patients (65%) showed clinical cure or improvement. Microbiological eradication of MRSA was confirmed in 14 patients (70%). ICU and hospital mortality rates were 30% and 35%, respectively. Maximum aerosolised vancomycin concentration was observed 4–5 h after nebulising (98.75 ± 21.79 mcg/mL). No additional systemic adverse effects occurred following aerosol vancomycin treatment. Aerosolised vancomycin combination therapy may be an alternative treatment for patients with severe MRSA pneumonia receiving mechanical ventilation (ClinicalTrials.gov number, NCT01925066).
Effects of Physical Exercises and Verbal Stimulation on the Functional Efficiency and Use of Free Time in an Older Population under Institutional Care: A Randomized Controlled Trial
Authors: Wiśniowska-Szurlej Agnieszka , Ćwirlej-Sozańska Agnieszka , Wołoszyn Natalia , Sozański Bernard , Wilmowska-Pietruszyńska Anna
Published: February 2020

Older people in institutional care are, for the most part, physically inactive and do not interact with each other or medical staff. Therefore, reducing sedentary behaviour is a new, important, and modifiable lifestyle variable that can improve the health of elderly people. The aim of the project was to assess the degree of improvement in functional performance and the possibility of changing habitual, free time behaviour among elderly people under institutional care by applying physical training with verbal stimulation. The study covered older people, aged 65–85 years, who are living a sedentary lifestyle in care homes in Southeastern Poland. Those who met the eligibility criteria were enrolled in the study and were assigned, at random, to one of four parallel groups: basic exercises (n = 51), basic exercises combined with verbal stimulation (n = 51), functional exercise training (n = 51), and functional exercise training with verbal stimulation (n = 51). No statistically significant differences in baseline characteristics were observed across the groups. Data were collected at baseline and at 12 and 24-weeks following the completion of the intervention. In the group with functional exercise training with verbal stimulation, in comparison to the group with basic exercises, the greatest positive short-term impact of intervention was demonstrated in terms of functional fitness (increased by 1.31 points; 95% confidence interval (CI) = 0.93–1.70), gait speed (improved by 0.17 m/s, 95% CI = 0.13–0.22), hand grip strength (by over 4 kg; 95% CI = 2.51–4.95), and upper-limb flexibility (by 10 cm; 95% CI = 5.82–12.65). There was also a significant increase in the level of free-time physical activity and an improvement in the quality of life, especially as expressed in the domain of overall physical functioning. Our study showed that a functional exercise program, combined with verbal stimulation, is effective at improving physical fitness and raising the level of free-time physical activity.
Osteo-Compatibility of 3D Titanium Porous Coating Applied by Direct Energy Deposition (DED) for a Cementless Total Knee Arthroplasty Implant: In Vitro and In Vivo Study
Authors: Ryu Dong Jin , Ban Hun Yeong , Jung Eui Yub , Sonn Chung-Hee , Hong Da Hee , Ahmad Shakra , Gweon Bomi , Lim Dohyung , Wang Joon Ho
Published: February 2020

Direct energy deposition (DED) technology has gained increasing attention as a new implant surface technology that replicates the porous structure of natural bones facilitating osteoblast colonization and bone ingrowth. However, concerns have arisen over osteolysis or chronic inflammation that could be caused by Cobalt-chrome (CoCr) alloy and Titanium (Ti) nanoparticles produced during the fabrication process. Here, we evaluated whether a DED Ti-coated on CoCr alloy could improve osteoblast colonization and osseointegration in vitro and in vivo without causing any significant side effects. Three types of implant CoCr surfaces (smooth, sand-blasted and DED Ti-coated) were tested and compared. Three cell proliferation markers and six inflammatory cytokine markers were measured using SaOS2 osteoblast cells. Subsequently, X-ray and bone histomorphometric analyses were performed after implantation into rabbit femur. There were no differences between the DED group and positive control in cytokine assays. However, in the 5-bromo-2′-deoxyuridine (BrdU) assay the DED group exhibited even higher values than the positive control. For bone histomorphometry, DED was significantly superior within the 1000 µm bone area. The results suggest that DED Ti-coated metal printing does not affect the osteoblast viability or impair osseointegration in vitro and in vivo. Thus, this technology is biocompatible for coating the surfaces of cementless total knee arthroplasty (TKA) implants.
Clinical Performance of Zygomatic Implants—Retrospective Multicenter Study
Authors: Davó Ruben , Bankauskas Simonas , Laurincikas Remigijus , Koçyigit Ismail Doruk , Mate Sanchez de Val José Eduardo
Published: February 2020

The main objective of this analysis was to evaluate (1) implant survival, (2) biologic complications, and (3) demographics associated with zygomatic implants placed according to the zygomatic anatomy-guided approach (ZAGA). This retrospective multicenter study reviewed data from the charts of 82 consecutive patients who had received 182 zygomatic implants. Patients were fully edentulous (62.2%), partially edentulous (22.0%), or had failing dentition (15.9%). Most patients (87.5%) did not have previous sinusitis and 11.3% had been previously treated for it. Additionally, about half of the patients (53.8%) did not present periodontal pathology, and one-third (36.3%) did, but were subsequently treated. Most implants (93.8%) were loaded immediately, i.e., within 48 h of placement. Implants were followed for 10.5 ± 7.2 months, and all were recorded as surviving and stable at last follow-up. Post-operative complications were infrequent and included sinusitis (10.1%) and peri-implant hyperplasia (0.8%). The low complication rate and 100% implant survival and stability indicate that zygomatic implants offer a viable treatment option when performing graftless restoration of severely resorbed maxilla, including immediate loading protocols.
Monitoring System of a Heat Pump Installation for Heating a Rural House Using Low-grade Heat from a Surface Watercourse
Authors: Kharchenko Valeriy , Sychov Arseniy , De Angelis Pasquale Luigi , Fiore Ugo
Published: February 2020

Increasing the efficiency of heat pump systems primarily used for heat supply to buildings is an important topic. This is especially true for systems constructed according to non-standard schemes and which use low-grade heat from various sources that are rarely considered for these purposes. Such studies require special, often expensive, data acquisition systems. In this paper, a low-cost computer-based monitoring system is presented. The monitoring system incorporates solutions which are new or seldom used. It is shown that modern semiconductor thermistors can replace commonly used platinum temperature sensors and thermocouples. A proposal for processing frequency output signals from sensors through an analog-to-digital converter and a way to reduce the number of required input channels are described. The monitoring system allows optimization of various types of heat-pump-based installations. The system has been used for quite a long time to monitor the operation of the heat pump installation using low-grade heat from a surface watercourse. With its help, the feasibility of using the previously proposed submersible floating heat exchanger is justified and the optimal scheme for its placement in the watercourse is determined.
Characterization of the Log-Normal Model for Received Signal Strength Measurements in Real Wireless Sensor Networks
Authors: Vallet García José
Published: February 2020

Using the classical received signal strength (RSS)-distance log-normal model in wireless sensor network (WSN) applications poses a series of characteristic challenges derived from (a) the model’s structural limitations when it comes to explaining real observations, (b) the inherent hardware (HW) variability typically encountered in the low-cost nodes of WSNs, and (c) the inhomogeneity of the deployment environment. The main goal of this article is to better characterize how these factors impact the model parameters, an issue that has received little attention in the literature. For that matter, I qualitatively elaborate on their effects and interplay, and present the results of two quantitative empirical studies showing how much the parameters can vary depending on (a) the nodes used in the model identification and their position in the environment, and (b) the antenna directionality. I further show that the path loss exponent and the reference power can be highly correlated. In view of all this, I argue that real WSN deployments are better represented by random model parameters jointly accounting for HW and local environmental characteristics, rather than by deterministic independent ones. I further argue that taking this variability into account results in more realistic models and plausible results derived from their usage. The article contains example values of the mean and standard deviation of the model parameters, and of the correlation between the path loss exponent and the reference power. These can be used as a guideline in other studies. Given the sensitivity of localization algorithms to the proper model selection and identification demonstrated in the literature, the structural limitations of the log-normal model, the variability of its parameters and their interrelation are all relevant aspects that practitioners need to be aware of when devising optimal localization algorithms for real WSNs that rely on this popular model.
Analysis of Super-Gentrification Dynamic Factors Using Interpretative Structure Modeling
Authors: Shi Jiangang , Duan Kaifeng , Xu Quanwei , Li Jiajia
Published: February 2020

The driving force of super-gentrification shapes a complex system in which multiple dynamic factors interact with each other. This paper takes the dynamic factor system of super-gentrification as the research object and uses the Interpretative Structure Modeling (ISM) to analyze these dynamic factors. The levels of these dynamic factors and the interaction between them are determined. The Cross Impact Matrix Multiplication Applied to a Classification (MICMAC) analysis is also conducted to determine the dependence power and driving power of these dynamic factors. Through analysis, it is concluded that the dynamic factors of super-gentrification are distributed on six levels. Among these dynamic factors, Transformation of Industrial Structure and Occupational Structure in Urban Central Areas, Housing Needs of Overseas Elites, Investment Needs, Development of the Real Estate Market, and Unique Areas and Lifestyle Preferences are the fundamental dynamic factors affecting super-gentrification. The findings of this paper can enrich the existing theoretical research on the driving force of super-gentrification and can provide a reference for policy makers to promote urban landscape sustainability to some extent.
Analysis of Land-Use Change between 2012–2018 in Europe in Terms of Sustainable Development
Authors: Gibas Piotr , Majorek Agnieszka
Published: February 2020

This article presents methodology of land use change assessment in the context of sustainable development and the results of its application based on the transformations that occurred in individual areas of Europe in the years 2012–2018. This method is based on data from the CORINE (CO-oRdination of INformation on Environment) Land Cover program) and local government units presenting the degree of urbanization (DEGURBA). The transformations taking place in space were evaluated and reduced to economic, social, and environmental dimensions. We then analyzed the results in terms of space (covering almost all of Europe) and in terms of division (large cities, small towns, suburbs, and rural areas). Results indicate that development of the economic dimension most often takes place at the expense of natural resources. It was also determined that the higher the population density, the greater the sustainable development differentiation level in the analyzed dimensions, of which the social dimension was characterized by the lowest differentiation and the economic dimension was highest. The development of rural areas was found to be less sustainable than large urban centers. Interpretation of the results also leads to the conclusion that areas of Europe are very diverse in terms of sustainable development. However, the method itself, despite the imperfections observed by the authors, may be used in further or similar studies.
Preparation of Black Ceramic Tiles Using Waste Copper Slag and Stainless Steel Slag of Electric Arc Furnace
Authors: Liu Mengke , Ma Guojun , Zhang Xiang , Liu Junjie , Wang Qiang
Published: February 2020

Copper slag and stainless steel slag of Electric Arc Furnace (EAF) are two typical metallurgical solid wastes, which contain a large number of valuables, such as Fe, Cr, and Cu. The transition metal elements in the waste slags, such as Cr and Fe, can be recycled as the coloring ions in the black ceramic tile. In this study, the Fe/Cr molar ratio in the raw materials of copper slag and stainless steel slag was adjusted, and the black ceramic tile was subsequently prepared by sintering. The results show that the optimum process parameters for the preparation of black ceramic tiles are the Fe/Cr molar ratio of 2.0, the sintering temperature of 1150 °C, and the sintering time of 30 min. The compressive strength of the black ceramic tile at optimum sintering conditions exceeds the minimum compressive strength of the Chinese national standard for standard polished tiles, and the concentrations of harmful elements, for example, Cr, Cu, Ni, As, Zn, Pb, and Cr(VI) are within the regulation thresholds specified by the Chinese national standard.
Erosion Corrosion Behavior of Aluminum in Flowing Deionized Water at Various Temperatures
Authors: Hao Liangshou , Zheng Feng , Chen Xiaoping , Li Jiayang , Wang Shengping , Fan Youping
Published: February 2020

To optimize the operating temperature and flow velocity of cooling water in a high voltage direct current (HVDC) thyristor valve cooling system, the erosion corrosion characteristics of aluminum electrodes in deionized water at various temperatures were studied. With increasing water temperature, the corrosion current of the aluminum electrode gradually increases and the charge transfer impedance gradually decreases, thus, the corrosion of aluminum tends to become serious. The aluminum electrode in 50 °C deionized water has the most negative corrosion potential (−0.930 V), the maximum corrosion current (1.115 × 10−6 A cm−2) and the minimum charge transfer impedance (8.828 × 10−6 Ω), thus, the aluminum corrosion at this temperature is the most serious. When the temperature of deionized water increases, the thermodynamic activity of the ions and dissolved oxygen in the deionized water increases, and the mass transfer process accelerates. Therefore, the electrochemical corrosion reaction of the aluminum surface will be accelerated. The corrosion products covering the aluminum electrode surface are mainly Al(OH)3. With increasing water temperature, the number of pits and grooves formed by corrosion on the aluminum surface increased. In this paper, the molar activation energy Ea and the equilibrium constant K of the aluminum corrosion reaction with various temperatures are calculated. This clarifies the effect of temperature on the aluminum corrosion reaction, which provides a basis for protecting aluminum from corrosion. The results of this study will contribute to research that is focused on the improvement of production techniques used for HVDC thyristor valve cooling systems.
Color Stability and Micro-Hardness of Bulk-Fill Composite Materials after Exposure to Common Beverages
Authors: Bahbishi Nora , Mzain Waad , Badeeb Bayan , Nassar Hani M.
Published: February 2020

Objectives: To assess the color stability and surface microhardness of Bulk-Fill composite materials available in the Saudi Arabia market. Methods: Five composite materials (Filtek Z350, Filtek Bulk-Fill, Tetric N-Ceram Bulk-Fill, Sonic Fill 2, and SDR) were investigated. Samples (n = 20; 10 mm in diameter and 2 mm in thickness) were fabricated using a stainless-steel mold and were immersed in tea, coffee, berry juice, and distilled water (control). Baseline (T0) shades of specimens were recorded using a spectrophotometer and after 10 (T1), 30 (T2), 60 (T3), and 90 days (T4) of immersion. Measurements were obtained against a black background and CIE L*a*b* data was used to calculate ΔE for each group. Vickers microhardness values were obtained at T0 and T4. Data was analyzed using mixed model repeated measure ANOVA at 0.05 significance level. Results: Time, material, and solution effects have statistically significant effect on ΔE. Tea was the most staining solution. Z350 was associated with the highest ΔE values while SDR showed the lowest values. No other materials showed significant difference between each other. Solutions were statistically different from each other. All materials were different from each other regarding microhardness. Conclusion: Bulk-Fill materials showed more color stability but lower microhardness values compared to universal resin control.
Recycling of Cigarette Butts in Fired Clay Bricks: A New Laboratory Investigation
Authors: Kurmus Halenur , Mohajerani Abbas
Published: February 2020

Cigarette butts (CBs) are the most commonly littered waste material in the world. It is estimated that over 5.7 trillion cigarettes are consumed worldwide each year. Consequently, millions of tonnes of highly toxic waste are contaminating the environment. CBs are composed of cellulose acetate filters—a polymer with poor biodegradability—and which, depending upon the environmental conditions, can take many years to decompose. In this study, fired clay bricks were manufactured with 0.5%, 1%, 1.5%, and 2% CBs by mass and tested against control bricks with 0% CBs. The results revealed a decrease in compressive strength from 48.6 MPa for 0% CB content bricks to 30.8 MPa for 1% CB content bricks, and a decrease in dry density with the increase in CB content, from 2114 kg/m3 for the control bricks to 1983 kg/m3 and 1969 kg/m3 for 1% and 2% CB content bricks. The highest value of water absorption appeared for 2% CB content bricks, which reached an absorption rate of 13.1% compared to 9% for the control bricks. The energy required during the firing process was calculated with a saving of up to 10.20%, for bricks incorporating 1% CBs. The thermal conductivity of the samples showed a reduction of 17% from 1.078 to 0.898 W m−1·K−1 with the addition of 1% CBs. In addition, the manufactured bricks were tested for efflorescence, an initial rate of absorption (IRA), microstructural analysis, and shrinkage. A life-cycle assessment (LCA) is recommended to analyze the environmental impacts of bricks incorporating CBs.
In-Situ Helium Implantation and TEM Investigation of Radiation Tolerance to Helium Bubble Damage in Equiaxed Nanocrystalline Tungsten and Ultrafine Tungsten-TiC Alloy
Authors: El Atwani Osman , Unal Kaan , Cunningham William Streit , Fensin Saryu , Hinks Jonathan , Greaves Graeme , Maloy Stuart
Published: February 2020

The use of ultrafine and nanocrystalline materials is a proposed pathway to mitigate irradiation damage in nuclear fusion components. Here, we examine the radiation tolerance of helium bubble formation in 85 nm (average grain size) nanocrystalline-equiaxed-grained tungsten and an ultrafine tungsten-TiC alloy under extreme low energy helium implantation at 1223 K via in-situ transmission electron microscope (TEM). Helium bubble damage evolution in terms of number density, size, and total volume contribution to grain matrices has been determined as a function of He+ implantation fluence. The outputs were compared to previously published results on severe plastically deformed (SPD) tungsten implanted under the same conditions. Large helium bubbles were formed on the grain boundaries and helium bubble damage evolution profiles are shown to differ among the different materials with less overall damage in the nanocrystalline tungsten. Compared to previous works, the results in this work indicate that the nanocrystalline tungsten should possess a fuzz formation threshold more than one order of magnitude higher than coarse-grained tungsten.
Detecting Extreme Values with Order Statistics in Samples from Continuous Distributions
Authors: Jäntschi Lorentz
Published: February 2020

In the subject of statistics for engineering, physics, computer science, chemistry, and earth sciences, one of the sampling challenges is the accuracy, or, in other words, how representative the sample is of the population from which it was drawn. A series of statistics were developed to measure the departure between the population (theoretical) and the sample (observed) distributions. Another connected issue is the presence of extreme values—possible observations that may have been wrongly collected—which do not belong to the population selected for study. By subjecting those two issues to study, we hereby propose a new statistic for assessing the quality of sampling intended to be used for any continuous distribution. Depending on the sample size, the proposed statistic is operational for known distributions (with a known probability density function) and provides the risk of being in error while assuming that a certain sample has been drawn from a population. A strategy for sample analysis, by analyzing the information about quality of the sampling provided by the order statistics in use, is proposed. A case study was conducted assessing the quality of sampling for ten cases, the latter being used to provide a pattern analysis of the statistics.
Robust Linear Trend Test for Low-Coverage Next-Generation Sequence Data Controlling for Covariates
Authors: Lee Jung Yeon , Kim Myeong-Kyu , Kim Wonkuk
Published: February 2020

Low-coverage next-generation sequencing experiments assisted by statistical methods are popular in a genetic association study. Next-generation sequencing experiments produce genotype data that include allele read counts and read depths. For low sequencing depths, the genotypes tend to be highly uncertain; therefore, the uncertain genotypes are usually removed or imputed before performing a statistical analysis. It may result in the inflated type I error rate and in a loss of statistical power. In this paper, we propose a mixture-based penalized score association test adjusting for non-genetic covariates. The proposed score test statistic is based on a sandwich variance estimator so that it is robust under the model misspecification between the covariates and the latent genotypes. The proposed method takes advantage of not requiring either external imputation or elimination of uncertain genotypes. The results of our simulation study show that the type I error rates are well controlled and the proposed association test have reasonable statistical power. As an illustration, we apply our statistic to pharmacogenomics data for drug responsiveness among 400 epilepsy patients.
Chaotic Synchronization Using a Self-Evolving Recurrent Interval Type-2 Petri Cerebellar Model Articulation Controller
Authors: Le Tien-Loc , Huynh Tuan-Tu , Nguyen Vu-Quynh , Lin Chih-Min , Hong Sung-Kyung
Published: February 2020

In this manuscript, the synchronization of four-dimensional (4D) chaotic systems with uncertain parameters using a self-evolving recurrent interval type-2 Petri cerebellar model articulation controller is studied. The design of the synchronization control system is comprised of a recurrent interval type-2 Petri cerebellar model articulation controller and a fuzzy compensation controller. The proposed network structure can automatically generate new rules or delete unnecessary rules based on the self-evolving algorithm. Furthermore, the gradient-descent method is applied to adjust the proposed network parameters. Through Lyapunov stability analysis, bounded system stability is guaranteed. Finally, the effectiveness of the proposed controller is illustrated using numerical simulations of 4D chaotic systems.
Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent
Authors: Gupta Dhanak , Silva Melissa , Radziun Karolina , Martinez Diana C. , Hill Christopher J. , Marshall Julie , Hearnden Vanessa , Puertas-Mejia Miguel A. , Reilly Gwendolen C.
Published: February 2020

Fucoidan is a brown algae-derived polysaccharide having several biomedical applications. This study simultaneously compares the anti-cancer activities of crude fucoidans from Fucus vesiculosus and Sargassum filipendula, and effects of low (LMW, 10–50 kDa), medium (MMW, 50–100 kDa) and high (HMW, >100 kDa) molecular weight fractions of S. filipendula fucoidan against osteosarcoma cells. Glucose, fucose and acid levels were lower and sulphation was higher in F. vesiculosus crude fucoidan compared to S. filipendula crude fucoidan. MMW had the highest levels of sugars, acids and sulphation among molecular weight fractions. There was a dose-dependent drop in focal adhesion formation and proliferation of cells for all fucoidan-types, but F. vesiculosus fucoidan and HMW had the strongest effects. G1-phase arrest was induced by F. vesiculosus fucoidan, MMW and HMW, however F. vesiculosus fucoidan treatment also caused accumulation in the sub-G1-phase. Mitochondrial damage occurred for all fucoidan-types, however F. vesiculosus fucoidan led to mitochondrial fragmentation. Annexin V/PI, TUNEL and cytochrome c staining confirmed stress-induced apoptosis-like cell death for F. vesiculosus fucoidan and features of stress-induced necrosis-like cell death for S. filipendula fucoidans. There was also variation in penetrability of different fucoidans inside the cell. These differences in anti-cancer activity of fucoidans are applicable for osteosarcoma treatment.
Quality of Life in Patients with Surgically Removed Skin Tumors
Authors: Răducu Laura , Avino Adelaida , Purnichescu Purtan Raluca , Balcangiu-Stroescu Andra-Elena , Bălan Daniela Gabriela , Timofte Delia , Ionescu Dorin , Jecan Cristian-Radu
Published: February 2020

Background and Objectives: Skin cancer is one of the most frequently diagnosed malignancies. The main goal of the therapeutic management is total excision with the prevention of recurrence and metastasis. The quality of life of the patients with skin cancer is affected by the morbidity risk, surgery, and cosmetic or functional aspects. The aim of this study was to evaluate the quality of life of patients with skin cancer prior to and post surgical intervention. Material and methods: We performed a prospective study on 247 patients with skin tumors. Quality of life was evaluated through an initial questionnaire that was given to all consenting patients. This was used to determine patients’ mobility, selfcare, normal activities, pain, and despair, using a five-point Likert scale. The general autoperceived health state was also recorded using a 100-point scale. The study included the responses of all patients at hospital admission, after one month of surgery, and after one year of surgery. Results: In patients with squamous cell carcinoma (SCC), the general health state indicator statistically significantly decreased one month after surgery and increased at one-year follow-up. In malignant melanoma (MM) patients, mobility, selfcare, normal activities, and discomfort presented a decrease in values one year after surgery, compared to the values registered at hospital admission. In patients with basal cell carcinoma (BCC), all indicators of quality of life presented an impaired value one year after surgery, after a decreasing trend. The general health state indicator statistically significantly increased one month after surgery and after one year. Conclusions: Surgery is one of the main steps in treating skin cancer. It has a great impact on patients’ quality of life because of pain andthe effect on mobility and normal activities. Skin cancers influence the quality of life of patients both psychologicallyand physically.
Safety and Tolerability of Ketamine Use in Treatment-Resistant Bipolar Depression Patients with Regard to Central Nervous System Symptomatology: Literature Review and Analysis
Authors: Włodarczyk Adam , Cubała Wiesław Jerzy
Published: February 2020

The current psychopharmacological treatment approaches for major depression focus on monoaminergic interventions, which are ineffective in a large proportion of patients. Globally, treatment-resistant bipolar depression (TRBD) affects up to 33% of depressive patients receiving treatment. Certain needs are still unmet and require new approaches. Many studies are in favor of treatments with ketamine, an N-methyl-D-aspartate (NMDA) receptor antagonist, even in single use, whose effects emerge in minutes to hours post administration. However, little data are available on ketamine performance in TRBD patients with somatic comorbidities, including highly prevalent ones, i.e., cardiovascular disease (heart failure, hypertension, post-myocardial infarct, arrhythmias, etc.) diabetes, and obesity, and depression-associated comorbidities such as stroke, epilepsy, as well as in the elderly population. The literature shows that treatment with ketamine is efficacious and safe, and the majority of adverse drug reactions are mild and tend to mostly disappear within 30 min to 2 h of ketamine administration.