bluuebunny/crossref_metadata_2025 huggingface dataset:
Disseminated Tuberculosis Associated Hemophagocytic Lymphohistiocytosis in a Pregnant Woman With Evans syndrome: A Case Report and Literature Review
Authors: Shi Yun-Feng , Shi Xiao-Han , Zhang Yuan , Chen Jun-Xian , Lai Wen-Xing , Luo Jin-Mei , Ba Jun-Hui , Wang Yan-Hong , Chen Jian-Ning , Wu Ben-Quan
Published: June 2021

Background

Tuberculosis (TB) is a leading cause of morbidity and mortality in underdeveloped and developing countries. Disseminated TB may induce uncommon and potentially fatal secondary hemophagocytic lymphohistiocytosis (HLH). Timely treatment with anti-tuberculosis therapy (ATT) and downmodulation of the immune response is critical. However, corticosteroid treatment for TB-associated HLH remains controversial. Herein, we report a successful case of disseminated TB-associated HLH in a pregnant woman with Evans syndrome accompanied by a literature review.

Case Presentation

A 26-year-old pregnant woman with Evans syndrome was transferred to the Third Affiliated Hospital of Sun Yat-Sen University because of severe pneumonia. She presented with cough, fever, and aggravated dyspnea. Nested polymerase chain reaction for Mycobacterium tuberculosis (M. tuberculosis) complex in sputum was positive. Sputum smear sample for acid-fast bacilli was also positive. Metagenome next-generation sequencing (mNGS) of the bronchoalveolar lavage fluid identified 926 DNA sequence reads and 195 RNA sequence reads corresponding to M. tuberculosis complex, respectively. mNGS of blood identified 48 DNA sequence reads corresponding to M. tuberculosis. There was no sequence read corresponding to other potential pathogens. She was initially administered standard ATT together with a low dose of methylprednisolone (40 mg/day). However, her condition deteriorated rapidly with high fever, acute respiratory distress syndrome, pancytopenia, and hyperferritinemia. Bone marrow smears showed hemophagocytosis. And caseating tuberculous granulomas were found in the placenta. A diagnosis of disseminated TB-associated HLH was made. Along with the continuation of four drug ATT regimen, therapy with a higher dose of methylprednisolone (160 mg/day) combined with immunoglobulin and plasma exchange was managed. The patient’s condition improved, and she was discharged on day 19. Her condition was good at follow-up with the continuation of the ATT.

Conclusions

Clinicians encountering patients with suspected TB accompanied by unexplainable inflammation not responding to ATT should consider complications with HLH. Timely administration of ATT combined with corticosteroids may result in a favorable outcome.
The Inhibitory Effect of Lysophosphatidylcholine on Proangiogenesis of Human CD34+ Cells Derived Endothelial Progenitor Cells
Authors: Zhao Haijun , He Yanhui
Published: June 2021

Increasing evidence reveals that lysophosphatidylcholine (LPC) is closely related to endothelial dysfunction. The present study aimed to investigate the mechanism of LPC in inhibiting the proangiogenesis and vascular inflammation of human endothelial progenitor cells (EPCs) derived from CD34+ cells. The early EPCs were derived from CD34+ hematopoietic stem cells whose purity was identified using flow cytometry analysis. The surface markers (CD34, KDR, CD31; VE-cadherin, vWF, eNOS) of EPCs were examined by flow cytometry analysis and immunofluorescence. RT-qPCR was used to detect the mRNA expression of inflammatory cytokines (CCL2, IL-8, CCL4) and genes associated with angiogenesis (VEGF, ANG-1, ANG-2) in early EPCs after treatment of LPC (10 μg/ml) or phosphatidylcholine (PC, 10 μg/ml, control). The angiogenesis of human umbilical vein endothelial cells (HUVECs) incubated with the supernatants of early EPCs was detected by a tube formation assay. The mRNA and protein levels of key factors on the PKC pathway (phosphorylated PKC, TGF-β1) were measured by RT-qPCR and western blot. The localization of PKC-β1 in EPCs was determined by immunofluorescence staining. We found that LPC suppressed the expression of CCL2, CCL4, ANG-1, ANG-2, promoted IL-8 expression and had no significant effects on VEGF expression in EPCs. EPCs promoted the angiogenesis of HUVECs, which was significantly inhibited by LPC treatment. Moreover, LPC was demonstrated to promote the activation of the PKC signaling pathway in EPCs. In conclusion, LPC inhibits proangiogenesis of human endothelial progenitor cells derived from CD34+ hematopoietic stem cells.
Sinomenine Suppresses Development of Hepatocellular Carcinoma Cells via Inhibiting MARCH1 and AMPK/STAT3 Signaling Pathway
Authors: Yang Wei , Feng Qihua , Li Minjing , Su Jiaqi , Wang Peiyuan , Wang Xu , Yin Yancun , Wang Xia , Zhao Mingdong
Published: June 2021

Promotion of apoptosis and suppression of proliferation in tumor cells are popular strategies for developing anticancer drugs. Sinomenine (SIN), a plant-derived alkaloid, displays antitumor activity. However, the mechanism of action of SIN against hepatocellular carcinoma (HCC) is unclear. Herein, several molecular technologies, such as Western Blotting, qRT-PCR, flow cytometry, and gene knockdown were applied to explore the role and mechanism of action of SIN in the treatment of HCC. It was found that SIN arrests HCC cell cycle at G0/G1 phase, induces apoptosis, and suppresses proliferation of HCC cells via down-regulating the expression of membrane-associated RING-CH finger protein 1 (MARCH1). Moreover, SIN induces cell death and growth inhibition through AMPK/STAT3 signaling pathway. MARCH1 expression was silenced by siRNA to explore its involvement in the regulation of AMPK/STAT3 signaling pathway. Silencing MARCH1 caused down-regulation of phosphorylation of AMPK, STAT3 and decreased cell viability and function. Our results suggested that SIN inhibits proliferation and promotes apoptosis of HCC cells by MARCH1-mediated AMPK/STAT3 signaling pathway. This study provides new support for SIN as a clinical anticancer drug and illustrates that targeting MARCH1 could be a novel treatment strategy in developing anticancer therapeutics.
Selective Reporting of Outcomes in Tinnitus Trials: Comparison of Trial Registries With Corresponding Publications
Authors: Beurden Isabeau van , Beek Megan J. van de , Heteren Jan A. A. van , Smit Adriana L. , Stegeman Inge
Published: June 2021

Objectives: We aimed to study the prevalence of selective reporting of primary and secondary outcomes in tinnitus trials and to examine if selective reporting of outcome measures is influenced by the nature and direction of its results.

Background: Selective reporting of outcome measures has been reported in several biomedical fields and can influence the clinical usefulness and implementation of outcomes of clinical trials. It is reported as one of the obstacles in finding an effective intervention for tinnitus.

Methods: ClinicalTrials.gov (CT.gov) was used to identify all registered interventional tinnitus trials up to December 2015. A standardized search was used to find corresponding publications up to March 2018. The prespecified outcomes in CT.gov were compared with the outcomes reported in corresponding publication(s). The effects of the (lack of) statistical significance of trial results and the effects of funding source on record adherence were evaluated. Changes in registration elements were assessed with the Archive site of CT.gov.

Results: We found corresponding publications for 60 (64.5%) of 93 eligible tinnitus trials registered in CT.gov. Of all the publications, five (7.5%) fully reported outcome measures entirely in line with the prespecified outcome measures. Discrepancies between the prespecified and reported outcomes were found in a total of 51 (76.1%) of the studies for primary outcomes, whereas 62 (92.5%) of the studies had discrepancies in secondary outcomes. In secondary outcomes, statistical significance of trial results influenced CT.gov record adherence. In addition, there was a statistically significant difference in the rate of discrepancy in industry-funded [n = 98 (87.5%) discrepant outcomes] and non-industry funded trials [n = 172 (74.5%) discrepant outcomes] (p = 0.01). Finally, 15 (25.9%) trialists made modifications in registered outcome measures during or after the trial period.

Conclusion: Tinnitus trials suffer from substantial outcome reporting bias. Awareness of its presence must be raised to limit the obstacles of finding an effective intervention for tinnitus.
A Novel Variation in the Mitochondrial Complex I Assembly Factor NDUFAF5 Causes Isolated Bilateral Striatal Necrosis in Childhood
Authors: Bi Hongyan , Guo Hui , Wang Qianfei , Zhang Xiao , Zhao Yaming , Li Jimei , Zhao Weiqin , Tuo Houzhen , Zhang Yongbo
Published: June 2021

Background: Bilateral striatal necrosis (BSN) is characterized by symmetrical degeneration, predominantly of the caudate and putamen nucleus, in the basal ganglia. It is associated with numerous acquired and hereditary neuro-developmental and motor dysfunction-related pathological conditions. BSN results in high morbidity and mortality among infants and children, and its diagnosis is clinically challenging due to several overlapping disease phenotypes. Therefore, a precise genetic diagnosis is urgently needed for accurate genetic counseling and improved prognostic outcomes as well.

Objective: To identify novel missense mutations in the NDUFAF5 gene as a cause of childhood BSN in members of a Chinese family and summarize the clinical characteristics of patients with the NDUFAF5 gene mutations.

Methods: This study included a large family living in a remote northwestern area of China. Three siblings developed a neurological disorder characterized by generalized dystonia within the first decade of their lives. Cerebral computed tomography (CT) and magnetic resonance imaging (MRI) showed bilateral lesions of the putamen. Biochemical and genetic approaches were used to identify the cause of BSN.

Results: Sequence analysis showed no pathogenic variation in PANK2, SLC25A19, SLC19A3, and NUP62 genes and in the entire mitochondrial genome as well. Whole-exome sequencing revealed compound heterozygous mutations consisting of NDUFAF5:c.425A > C(p.E142A) and c.836T > G (p.M279R). The father, a healthy sister, and a healthy brother of the affected siblings carried the c.836T > G mutation, and the mother carried the c.425A > C mutation. These variants were absent in 100 ethnically matched non-BSN controls. In silico analysis demonstrated that the E142A and M279R mutations in NDUFAF5 protein significantly perturbed the normal conformation of the protein due to alterations in the hydrogen bonding patterns around the evolutionarily conserved catalytic domains, leading to its loss of function in the early stage of mitochondrial complex I assembly.

Conclusions: We identified a novel compound heterozygous mutation (c.425A > C and c.836T > G) in the NDUFAF5 gene as the potential cause of autosomal recessive childhood BSN, which extended the pathogenic variation spectrum of the NDUFAF5 gene. This study provides substantial evidence for further improvement of genetic counseling and better clinical management of BSN affected individuals.
LLGL2 Increases Ca2+ Influx and Exerts Oncogenic Activities via PI3K/AKT Signaling Pathway in Hepatocellular Carcinoma
Authors: Leng Shusheng , Xie Fei , Liu Junyi , Shen Junyi , Quan Guangqian , Wen Tianfu
Published: June 2021

Background

Lethal giant larvae (Lgl), scaffolding proteins, regulate the epithelial cell apicobasal polarity in Drosophila. They play important roles in asymmetric cell division, cell migration, and progenitor cells self-renewal as tumor suppressors. One of Lgl mammalian homologues proteins, LLGL2 overexpression has been reported in ER+ breast cancer and promotes tumor proliferation through regulating leucine uptake. Nonetheless, the role of LLGL2 in hepatocellular carcinoma (HCC) is still unknown.

Methods

TCGA dataset mining, qRT-PCR, Western blot along with immunohistochemistry assays were employed to explore LLGL2 expression in human HCC samples and cell lines. Moreover, the clinical value of LLGL2 was investigated in 156 HCC patients. Furthermore, the role as well as the molecular mechanism of LLGL2 in the progression of HCC was explored through a series of in vitro and in vivo experiments.

Results

LLGL2 was up-regulated in HCC tissues, which was related with certain clinicopathological features including tumor number, vascular invasion as well as advanced stage. High expression of LLGL2 predicted poor prognosis after hepatectomy. LLGL2 promoted HCC cells proliferation, migration and invasion through PI3K/ATK signaling by promoting calcium ion influx.

Conclusion

Our study identified that LLGL2 is a tumor promoter in HCC for the first time, which could potentially be utilized as a new biomarker and a therapeutic target for HCC.
Evaluation of Epigallocatechin-3-Gallate as a Radioprotective Agent During Radiotherapy of Lung Cancer Patients: A 5-Year Survival Analysis of a Phase 2 Study
Authors: Zhu Wanqi , Zhao Yalan , Zhang Shuyu , Li Xiaolin , Xing Ligang , Zhao Hanxi , Yu Jinming
Published: June 2021

Background

Previous analysis of the study (NCT02577393) had demonstrated the application of epigallocatechin-3-gallate (EGCG) could be safe and effective in the prevention and treatment of acute radiation esophagitis in patients with advanced lung cancer. EGCG seemed to improve the response rate of small cell lung cancer (SCLC) to radiotherapy in a subgroup analysis. This research continued to analyze the impact of EGCG application on cancer-radiation efficacy and patient survival.

Methods

All patients with SCLC in the NCT02577393 study were included. Patients were randomized into EGCG group or conventional therapy group as protocol. The primary endpoints of the study were radiation response rate and progression-free survival (PFS). Overall survival (OS) and the efficacy of EGCG in the treatment of esophagitis were assessed as secondary endpoints.

Results

A total of 83 patients with lung cancer in the NCT02577393 study were screened, and all 38 patients with SCLC were eligible for analysis. No significant differences with regard to baseline demographic and clinical characteristics were observed between the two groups. The objective response rate (ORR) was higher than that of conventionally treated patients (84.6 vs 50%, P = 0.045), while the median PFS and OS were not significantly prolonged. At data cut-off (1 January 2021), 5-year PFS was 33% with EGCG versus 9.3% with conventional treatment, and 5-year OS was 30.3% versus 33.3%, respectively. The mean adjusted esophagitis index and pain index of patients with EGCG application were lower than conventional treatment (5.15 ± 2.75 vs 7.17 ± 1.99, P = 0.030; 8.62 ± 5.04 vs 15.42 ± 5.04, P < 0.001).

Conclusion

The study indicates EGCG may alleviate some esophagitis-related indexes in SCLC patients exposed to ionizing radiation without reducing survival. However, this conclusion should be confirmed by further studies with large sample size.
Self-Nanoemulsifying Drug Delivery System Loaded with Psiadia punctulata Major Metabolites for Hypertensive Emergencies: Effect on Hemodynamics and Cardiac Conductance
Authors: Abdallah Hossam M. , El-Bassossy Hany M. , El-Halawany Ali M. , Ahmed Tarek A. , Mohamed Gamal A. , Malebari Azizah M. , Hassan Noura A.
Published: June 2021

Vasodilators are an important class of antihypertensive agents. However, they have limited clinical use due to the reflex tachycardia associated with their use which masks most of its antihypertensive effect and raises cardiac risk. Chemical investigation of Psiadia punctulata afforded five major methoxylated flavonoids (1–5) three of which (1, 4, and 5) showed vasodilator activity. Linoleic acid-based self-nanoemulsifying drug delivery system (SNEDDS) was utilized to develop intravenous (IV) formulations that contain compounds 1, 4, or 5. The antihypertensive effect of the prepared SNEDDS formulations, loaded with each of the vasodilator compounds, was tested in the angiotensin-induced rat model of hypertension. Rats were subjected to real-time recording of blood hemodynamics and surface Electrocardiogram (ECG) while the pharmaceutical formulations were individually slowly injected in cumulative doses. Among the tested formulations, only that contains umuhengerin (1) and 5,3′-dihydroxy-6,7,4′,5′-tetramethoxyflavone (5) showed potent antihypertensive effects. Low IV doses, from the prepared SNEDDS, containing either compound 1 or 5 showed a marked reduction in the elevated systolic blood pressure by 10 mmHg at 12 μg/kg and by more than 20 mmHg at 36 μg/kg. The developed SNEDDS formulation containing either compound 1 or 5 significantly reduced the elevated diastolic, pulse pressure, dicrotic notch pressure, and the systolic–dicrotic notch pressure difference. Moreover, both formulations decreased the ejection duration and increased the non-ejection duration while they did not affect the time to peak. Both formulations did not affect the AV conduction as appear from the lack of effect on p duration and PR intervals. Similarly, they did not affect the ventricular repolarization as no effect on QTc or JT interval. Both formulations decreased the R wave amplitude but increased the T wave amplitude. In conclusion, the careful selection of linoleic acid for the development of SNEDDS formulation rescues the vasodilating effect of P. punctulata compounds from being masked by the reflex tachycardia that is commonly associated with the decrease in peripheral resistance by most vasodilators. The prepared SNEDDS formulation could be suggested as an effective medication in the treatment of hypertensive emergencies, after clinical evaluation.
Global Analysis of UDP Glucose Pyrophosphorylase (UDPGP) Gene Family in Plants: Conserved Evolution Involved in Cell Death
Authors: Liu Shuai , Zhong Hua , Wang Qiang , Liu Caixiang , Li Ting , Peng Zhaohua , Li Yangsheng , Zhang Hongyu , Liao Jianglin , Huang Yingjin , Wang Zhaohai
Published: June 2021

UDP glucose pyrophosphorylase (UDPGP) family genes have been reported to play essential roles in cell death or individual survival. However, a systematic analysis on UDPGP gene family has not been performed yet. In this study, a total of 454 UDPGP proteins from 76 different species were analyzed. The analyses of the phylogenetic tree and orthogroups divided UDPGPs into three clades, including UDP-N-acetylglucosamine pyrophosphorylase (UAP), UDP-glucose pyrophosphorylase (UGP, containing UGP-A and UGP-B), and UDP-sugar pyrophosphorylase (USP). The evolutionary history of the UDPGPs indicated that the members of UAP, USP, and UGP-B were relatively conserved while varied in UGP-A. Homologous sequences of UGP-B and USP were found only in plants. The expression profile of UDPGP genes in Oryza sativa was mainly motivated under jasmonic acid (JA), abscisic acid (ABA), cadmium, and cold treatments, indicating that UDPGPs may play an important role in plant development and environment endurance. The key amino acids regulating the activity of UDPGPs were analyzed, and almost all of them were located in the NB-loop, SB-loop, or conserved motifs. Analysis of the natural variants of UDPGPs in rice revealed that only a few missense mutants existed in coding sequences (CDSs), and most of the resulting variations were located in the non-motif sites, indicating the conserved structure and function of UDPGPs in the evolution. Furthermore, alternative splicing may play a key role in regulating the activity of UDPGPs. The spatial structure prediction, enzymatic analysis, and transgenic verification of UAP isoforms illustrated that the loss of N- and C-terminal sequences did not affect the overall 3D structures, but the N- and C-terminal sequences are important for UAP genes to maintain their enzymatic activity. These results revealed a conserved UDPGP gene family and provided valuable information for further deep functional investigation of the UDPGP gene family in plants.
A Physiotherapeutic Approach to Musicians' Health – Data From 614 Patients From a Physiotherapy Clinic for Musicians (INAP/O)
Authors: Zalpour Christoff , Ballenberger Nikolaus , Avermann Florian
Published: June 2021

Currently, the treatment of musicians is an interprofessional approach. Playing-related health complaints may impact the performance of a musician. In Germany, a medical consulting hour for musicians exists, but those for athletes in sports medicine are not so common. The diagnosing and treatment procedure within the physiotherapy consultation for musicians follows a specific concept-b and requires knowledge of instruments and musician-specific complaints. Based on the consulting hour in a clinic in Osnabrueck, 614 case reports were part of this sample, of which 558 data sets were complete. The focus of the analysis is the instrument and the primary complaint. Also, the type of therapy is characterized, and the amount is calculated. Primary complaints of musicians, in general, are found most frequently in the spine and upper extremity. Musician complaints are different between instruments. Instrumentalists have a significantly higher chance to suffer from a primary complaint in the area of the upper extremity. Furthermore, the groups without an instrument (e.g., singing or dancing) are developing complaints in the anatomical area which they primarily use. Therefore, these types of therapy were used: physiotherapy, manual therapy, and osteopathy with an average of 5.9 treatment units. This study underpinned the importance of musician-specific physiotherapy as a profession to treat musicians. Also, an interdisciplinary approach is necessary to treat all aspects of complaints.
Students’ Class Perceptions and Ratings of Instruction: Variability Across Undergraduate Mathematics Courses
Authors: Wilkins Jesse L. M. , Jones Brett D. , Rakes Lee
Published: June 2021

The primary purpose of this study was to examine whether students’ motivation-related perceptions of mathematics courses were related to their ratings of instruction while controlling for their academic major, type of math class, and expected grade in the class. We investigated these relationships at both the student- and class-level because little is known about whether students’ motivation-related perceptions vary across mathematics courses and whether this variance is related to overall class ratings of instruction. The sample included 795 students nested within 43 different mathematics course sections. Students provided their course perceptions of autonomy, utility value, expectancies for success, situational interest, instructor caring, expected grade, and their overall perceptions of the course and instructor. Multilevel modeling techniques were used to investigate potential student- and class-level effects as well as compositional effects. Students’ class perceptions varied significantly across mathematics courses. In addition, students’ motivation-related course perceptions were positively related to their instructor and course ratings at both the student-level and class-level; however, the strength of these relationships sometimes varied across courses for some of the motivation-related perceptions. These results suggest that the motivational climate (i.e., the psychological environment) can affect students’ instructor and course ratings. Moreover, these findings suggest that instructors have some control over their instructor and course ratings through the teaching strategies that they implement. For example, they may be able to increase their ratings by implementing teaching strategies that support students’ autonomy, goals, success, interests, and relationships.
Insights Into Co-Morbidity and Other Risk Factors Related to COVID-19 Within Ontario, Canada
Authors: Snider Brett , Patel Bhumi , McBean Edward
Published: June 2021

The worldwide rapid spread of the severe acute respiratory syndrome coronavirus 2 has affected millions of individuals and caused unprecedented medical challenges by putting healthcare services under high pressure. Given the global increase in number of cases and mortalities due to the current COVID-19 pandemic, it is critical to identify predictive features that assist identification of individuals most at-risk of COVID-19 mortality and thus, enable planning for effective usage of medical resources. The impact of individual variables in an XGBoost artificial intelligence model, applied to a dataset containing 57,390 individual COVID-19 cases and 2,822 patient deaths in Ontario, is explored with the use of SHapley Additive exPlanations values. The most important variables were found to be: age, date of the positive test, sex, income, dementia plus many more that were considered. The utility of SHapley Additive exPlanations dependency graphs is used to provide greater interpretation of the black-box XGBoost mortality prediction model, allowing focus on the non-linear relationships to improve insights. A “Test-date Dependency” plot indicates mortality risk dropped substantially over time, as likely a result of the improved treatment being developed within the medical system. As well, the findings indicate that people of lower income and people from more ethnically diverse communities, face an increased mortality risk due to COVID-19 within Ontario. These findings will help guide clinical decision-making for patients with COVID-19.
Advances in Food Processing (Food Preservation, Food Safety, Quality and Manufacturing Processes)
Authors: Varzakas Theodoros , Tsarouhas Panagiotis
Published: June 2021

The aim of this special issue was to bring about advances in the area of food manufacturing, including packaging, addressing issues of food safety, quality, fraud and how these processes (new and old) could affect the organoleptic characteristics of foods, with the aim of promoting consumer satisfaction [...]
Microscopic-Phenomenological Model of Glass Transition and Temperature Dependence of Viscosity—Part I: Foundations of the Model
Authors: Sturm Karl Günter
Published: June 2021

The glass transition is described as a time- and history-independent singular event, which takes place in an interval dependent on the distribution width of molecular vibration amplitudes. The intrinsic glass transition is not seen as a relaxation phenomenon, but is characterized by a fixed volumetric state at the glass temperature Tg0. The relaxation behavior of the transport properties depends on the distance to Tg0. Free volume is redefined and its generation is the result of the fluctuating transfer of thermal energy into condensed matter and the resulting combined interactions between the vibration elements. This creates vacancies between the elements which are larger than the cross-section of an adjacent element or parts thereof. Possible shifts of molecules or molecular parts through such apertures depend on the size and axis orientation and do not require further energetic activation. After a displacement, additional volume is created by delays in occupying abandoned positions and restoring the energetic equilibrium. The different possibilities of axis orientation in space result in the different diffusive behavior of simple molecules and chain molecules, silicate network formers, and associated liquids. Glass transformation takes place at a critical volume Vg0 when the cross-section of apertures becomes smaller than the cross-section of the smallest molecular parts. The glass transition temperature Tg0 is assigned to Vg0 and is therefore independent of molecular relaxation processes. Tg0 is well above the Kauzmann and Vogel temperatures, usually just a few degrees below the conventionally measured glass temperature Tg(qT). The specific volume at the two temperatures mentioned above cannot be achieved by a glass with an unordered structure but only with aligned molecular axes, i.e. in a crystalline state. Simple liquids consisting of non-spherical molecules additionally alter their behavior above Vg0 at Vgl where the biggest gaps are as small as the largest molecular diameter. Tgl is located in the region of the crystalline melting point Tm. Both regions, above and below Tm, belong to different physical states and have to be treated separately. In the region close to Vg0 respectively Tg0, the distribution of vibration amplitudes has to be taken into account. The limiting volume Vg0 and the formation of apertures larger than the cross-section of the vibrating elements or parts thereof, in conjunction with the distribution width of molecular vibrations as Vg0 is approached, and the spatial orientation of the molecular axes is key to understanding the glass transition.
Correction: Lochbaum et al. The Profile of Moods States and Athletic Performance: A Meta-Analysis of Published Studies. Eur. J. Investig. Health Psychol. Educ. 2021, 11, 50–70
Authors: Lochbaum Marc , Zanatta Thaís , Kirschling Deylon , May Emily
Published: June 2021

Error in Section 2.6 [...]
Mental Health of Teachers Who Have Teleworked Due to COVID-19
Authors: Palma-Vasquez Claudia , Carrasco Diego , Hernando-Rodriguez Julio C.
Published: June 2021

The impact of the COVID-19 pandemic on education included school closures and the implementation of virtual teaching and teleworking without the knowledge or resources needed to do so. This situation accentuated the inequality in accessing quality education and generated high rates of stress, anxiety, and general discomfort in teachers. This study aimed to explore the mental health of teachers who were forced to telework because of COVID-19, and to analyze the association with sociodemographic, teacher-related, and working conditions. The sample was 278 classroom teachers in Chile who teleworked more than 50% during the 2020 academic year. The participants were mostly women (82%) who entered the teaching profession at age 30 or younger (87%) and worked two or more unpaid overtime hours per day (67%). The dependent variable was mental health measured through the General Health Questionnaire (GHQ-12). The independent variables were sociodemographic, teacher-related, and work conditions. The internal structure of the mental health construct was evaluated using the Rasch model. Crude odds ratios (cORs) and adjusted odds ratios (aORs) were estimated using logistic regression models. A high rate of poor mental health was identified in teachers (58%). The variables associated with poor mental health were working in a private–subsidized school (aOR = 2.89; 95% CI: 1.16–7.22), working two or more unpaid overtime hours (aOR = 2.25; 95% CI: 1.11–4.59), and being absent due to sickness (aOR = 3.82; 95% CI: 1.53–9.58). These results provide evidence suggesting the need for actions to improve the working conditions of teachers who telework in order to improve their mental health, and thus have a positive impact on the entire educational community.
Multiperspectivity as a Process of Understanding and Reflection: Introduction to a Model for Perspective-Taking in Geography Education
Authors: Vasiljuk Dina , Budke Alexandra
Published: June 2021

Perspective-taking is an essential competency because it enables a better understanding of complex issues or conflicts with various actors and different points of view involved. However, no competency model for perspective-taking has been provided in geography education to date, which is why a respective model was developed in this study. The model was then applied by analysing 28 articles from four practice journals of geography education from German-speaking countries. This analysis focused on the dimensions of the perspective-taking competencies that were required by respective tasks within the lesson concepts. The results show that the tasks hardly promoted competence-oriented geography teaching in terms of perspective-taking. Therefore, the competency model could be a suitable tool for analysing and developing teaching materials that implement perspective-taking.
Relationship between Cycling Infrastructure and Transportation Cycling in a Small Urban Area
Authors: Larouche Richard , Patel Nimesh , Copeland Jennifer L.
Published: June 2021

The role of infrastructure in encouraging transportation cycling in smaller cities with a low prevalence of cycling remains unclear. To investigate the relationship between the presence of infrastructure and transportation cycling in a small city (Lethbridge, AB, Canada), we interviewed 246 adults along a recently-constructed bicycle boulevard and two comparison streets with no recent changes in cycling infrastructure. One comparison street had a separate multi-use path and the other had no cycling infrastructure. Questions addressed time spent cycling in the past week and 2 years prior and potential socio-demographic and psychosocial correlates of cycling, including safety concerns. Finally, we asked participants what could be done to make cycling safer and more attractive. We examined predictors of cycling using gender-stratified generalized linear models. Women interviewed along the street with a separate path reported cycling more than women on the other streets. A more favorable attitude towards cycling and greater habit strength were associated with more cycling in both men and women. Qualitative data revealed generally positive views about the bicycle boulevard, a need for education about sharing the road and for better cycling infrastructure in general. Our results suggest that, even in smaller cities, cycling infrastructure may encourage cycling, especially among women.
Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay
Authors: Elfadul Rehab , Jesien Roman , Elnabawi Ahmed , Chigbu Paulinus , Ishaque Ali
Published: June 2021

Contaminants of Emerging Concern (CECs) with estrogenic or estrogenic-like activity have been increasingly detected in aquatic environments and have been an issue of global concern due to their potential negative effects on wildlife and human health. This study used the MCF-7 cell proliferation assay (E-Screen) to assess the estrogenic activity profiles in Maryland Coastal Bays (MCBs), a eutrophic system of estuaries impacted by human activities. Estrogenic activity was observed in all study sites tested. Water samples from MCBs increased MCF-7 cell proliferation above the negative control from 2.1-fold at site 8, located in Sinepuxent Bay close to the Ocean City Inlet, to 6.3-fold at site 6, located in Newport Bay. The proliferative effects of the sediment samples over the negative control ranged from 1.9-fold at the Assateague Island National Seashore site to 7.7-fold at the Public Landing site. Moreover, elevated cell proliferation (p < 0.05) was observed when cells were co-exposed with 17ß-Estradiol (E2), while reduction in cell proliferation was observed when cells were co-exposed with the antagonist ICI 182, 780 suggesting that cell proliferative effects were primarily mediated by the estrogen receptor (ER). These results suggest the occurrence of some estrogenic or hormonal-like compounds in the MCBs and are consistent with our previous findings based on vitellogenin analyses.
Impact of Nutrition-Based Interventions on Athletic Performance during Menstrual Cycle Phases: A Review
Authors: Helm Macy M. , McGinnis Graham R. , Basu Arpita
Published: June 2021

Despite the steady increase in female participation in sport over the last two decades, comprehensive research on interventions attenuating the influence of female menstrual physiology on performance remains scarce. Studies involving eumenorrheic women often only test in one menstrual phase to limit sex hormone variance, which may restrict the application of these findings to the rest of the menstrual cycle. The impacts of nutrition-based interventions on athletic performance throughout the menstrual cycle have not been fully elucidated. We addressed this gap by conducting a focused critical review of clinical studies that reported athletic outcomes as well as menstrual status for healthy eumenorrheic female participants. In total, 1443 articles were identified, and 23 articles were included. These articles were published between 2011 and 2021, and were retrieved from Google Scholar, Medline, and PubMed. Our literature search revealed that hydration-, micronutrient-, and phytochemical-based interventions can improve athletic performance (measured by aerobic capacity, anaerobic power, and strength performance) or attenuate exercise-induced damage (measured by dehydration biomarkers, muscle soreness, and bone resorption biomarkers). Most performance trials, however, only assessed these interventions in one menstrual phase, limiting the application throughout the entire menstrual cycle. Improvements in athletic performance through nutrition-based interventions may be contingent upon female sex hormone variation in eumenorrheic women.
Emerging Biomarkers for the Selection of Advanced NSCLC-Affected Immunotherapy Patients
Authors: Della Gravara Luigi , Battiloro Ciro , Letizia Antonietta , Cantile Rosa , D'Agnano Vito , Sica Giacomo , Rocco Danilo
Published: June 2021

Immunotherapy in the form of ICIs has revolutionized advanced NSCLC treatment algorithms, with ICI-containing combination treatments being the latest addition to approved regimens. However, PD-L1 still represents the only routinely assessed and validated biomarker apart from genetic drivers testing, impairing our capacity to personalize and guide treatment. Therefore, this paper aims to analyze the most promising emerging predictive biomarkers that could help us in the near future to select patients more effectively.
Prussian Blue and Carbon-Dot Hybrids for Enhanced Electrochromic Performance
Authors: Chu Jia , Cheng Yaping , Li Xue , Yang Fan , Xiong Shanxin , Zhang Zhao
Published: June 2021

In this study, Prussian blue@Carbon-dot (PB@C-dot) hybrids have been developed by one-step hydrothermal method. The incorporation of C-dots into Prussian blue thin film as a way of improving its electrochromic performance was investigated. The structure of the PB@C-dot hybrid was characterized through X-ray diffraction, Raman spectroscopy and scanning electron microscopy. The electrochromic properties showed that incorporation of 10 mL C-dots into the film showed higher optical contrast of 1.6 and superior coloration/bleaching response of 10 and 3 s. It is proposed that the C-dots component used in the construction of the PB@C-dot hybrid plays a key role to achieve superior electrochromic performance.
A Layer-Arranged Meshless Method for the Simulation of Additive Manufacturing with Irregular Shapes
Authors: Lee Ming-Hsiao , Chen Wen-Hwa , Mao Ying
Published: June 2021

Additive manufacturing (3D Printing) has become a promising manufacturing method as it can produce parts in a flexible and efficient way, especially for very irregular parts. However, during the printing process, the material experiences a great temperature change from the melting temperature to room temperature; this causes high thermal strains and induces distinct deformations which degrade the quality of the printed parts, especially in metal 3D printing. In order to reduce possible problems and find possible solutions, a prior evaluation by simulation is often adopted. Nevertheless, since the 3D printing process generates parts in a layer-by-layer way, the analysis model should also be layer-by-layer arranged and used with a layer-by-layer based analysis process to simulate the layer-by-layer additive printing; otherwise, the simulation may not match the real behavior. In order to meet these requirements, a new meshless method is proposed to match the situations and handle these problems. As a meshless method, the modeling is not constrained by the element distribution. In addition, the analysis model generated with the proposed method can be arranged in a layer-by-layer way and combined with the proposed layer-by-layer analysis scheme, so it can then match and simulate the printing processes. Furthermore, the layer-by-layer arranged models can be automatically created, directly based on the STL (STereo-Lithography) geometry model, which is a de facto standard in the 3D printing industry. This makes the proposed approach more straightforward and efficient. To validate the proposed method, two parts with holes inside have been printed and simulated for comparison. The results show a good agreement. In addition, a highly irregular part has also been simulated to demonstrate the effectiveness and efficiency of this proposed method.
Experimental Investigation on a Bladed Disk with Traveling Wave Excitation
Authors: Carassale Luigi , Rizzetto Elena
Published: June 2021

Bladed disks are key components of turbomachines and their dynamic behavior is strongly conditioned by their small accidental lack of symmetry referred to as blade mistuning. The experimental identification of mistuned disks is complicated due to several reasons related both to measurement and data processing issues. This paper describes the realization of a test rig designed to investigate the behavior of mistuned disks and develop or validate data processing techniques for system identification. To simplify experiments, using the opposite than in the real situation, the disk is fixed, while the excitation is rotating. The response measured during an experiment carried out in the resonance-crossing condition is used to compare three alternative techniques to estimate the frequency-response function of the disk.
Evolution of Rabies in South America and Inter-Species Dynamics (2009–2018)
Authors: Meske Mauro , Fanelli Angela , Rocha Felipe , Awada Lina , Soto Paula Caceres , Mapitse Neo , Tizzani Paolo
Published: June 2021

Rabies is listed as one of the World Health Organisation’s (WHO) Neglected Tropical Diseases Worldwide, with a significant impact in South America. This paper explores the dynamics of rabies cases in humans, pets (dogs and cats), livestock and wildlife (bats in particular) in South America during the period 2009–2018. The data used in this study were derived from the two main databases for rabies in South America: the OIE-WAHIS from the World Organisation for Animal Health (OIE) and PANAFTOSA’s Regional Information System for the Epidemiological Surveillance of Rabies (SIRVERA). Being a neglected disease with possible underreporting in some areas, the reported rabies cases may not always represent the real disease burden. The analysis focuses on the evolution of the number of cases in time and their spatial distribution, as well as on the main source of infections in humans, determined by laboratory assays of the antigenic variant or through epidemiological investigations. Additionally, Generalised Linear Mixed Models (GLMM) were used to evaluate the risk factors associated with the occurrence of human cases. Our results show that the highest impact of the disease in terms of number of cases was reported on livestock, while the overall number of cases (in animals and humans) progressively decreased along the study period. The spatial distribution of rabies in livestock showed two main clusters in the north-western (mainly Colombia) and in the south-eastern part of the affected area (Brazil), and a third smaller cluster in Peru. A cluster in dogs was observed in Bolivia. Out of the 192 human cases reported during the study period, 70% of them were transmitted by bats. The number of human cases reported during the study period were significantly associated with the number of rabies cases reported in livestock, pets and wildlife. Despite the overall decreasing case report rate, the disease still represents a major animal and public health concern in South America, and new strategies for compiling systematic information, networking and education are needed, as well as the education and training of veterinary staff.
The basis of food security of the country is the potential of the agricultural sector of the regional economics
Authors: Gushchenskaya N. D. , Sumarokova M. A.
Published: June 2021

The large territorial extent of our country has formed a certain resource potential for each region, which depends on the geographical, natural and climatic conditions, as well as the competitive advantages of the territory. The purpose of the research was to assess the degree of influence of the use of the resource potential of the territory on its food security in order to predict possible consequences when one of the determinants changes. The availability and use of production resources in the agricultural sector of the economics in the Kurgan region has been analyzed in the article. The evaluation of food security of the territory is based on the use of a set of indicators that reflect the physical and economics availability of food, the sufficiency and quality of consumption. Agricultural production had been increased by more than 30 % during the analyzed period, and the inflationary factor should be taken into account in this growth. With the existing programs to support agricultural producers there is no significant growth in the number of cattle and the area of crops in the region. According to the threshold values of the level of food self-sufficiency laid down by the Food Security Doctrine of the Russian Federation the current state of agriculture in the Kurgan region cannot meet the region’s demand for meat and meat products. In 2019 the level of food independence for grain was 174,4 %, and for potatoes – 105,8 %, which indicates the feasibility of exporting these types of agricultural products to other regions of Russia. The annual grain export potential in the Kurgan region is estimated in the range from 0,6 to 1 million tons of grain. The conclusions made allow us to assess the degree of correlation between the resource potential of the region and the level of food security, and to develop recommendations for improving food self-sufficiency.
Staff motivation as a growth factor labor productivity and efficiency in the service sector
Authors: Baranova S. , Golovina L. P. , Lidinfa E. , Panin A.
Published: June 2021

The scientifi c article deals with the organization of the functioning of retail retail companies. The possibility of using marketing tools in personnel management in the "Trade" industry is shown. Recommendations are given for the formation of key Performance Indicators (KPI) when evaluating the quality of professional tasks performed by employees in the industry. The analytical basis confi rming the signifi cance, relevance and need to adjust the terms of use of the KPI system in the Trade industry was the offi cial information posted on the websites of retail retail companies and customer reviews of the Austin LLC, Zolla LLC and Gloria Jeans JSC store chains. The scientifi c article presents the author’s subjective opinion on the degree of customer satisfaction with the organization of the functioning of retail retail companies included in the research base
Accounting of production of finished products in agricultural organizations
Authors: Tunin Sergey Alexandrovich , Frolov Alexander Vitalievich , Dzhikiya Konstantin Akakievich
Published: June 2021

The activity of agricultural organizations involves the implementation of the production process in order to obtain finished products on the basis of expanded reproduction as the main source of funds for the organization. An important point here is the correct accounting of finished products, taking into account the specifics of the activities of agricultural organizations. The significance of this area of accounting work of an agricultural organization was the fundamental point in choosing the topic of the research.