bluuebunny/crossref_metadata_2025 huggingface dataset:
Kesiapan Kompetensi Profesional Pustakawan dalam Pembangunan dan Pengembangan iKuansing di Dispersip Kuansing
Authors: Marlinda  , Prabowo Thoriq Tri
Published: November 2022

This study aims to determine the readiness of the professional competence of librarians in the development and development of iKuansing in the Dispersip Kuansing. The results of this study are presented in accordance with Sudarsono's theory, namely the readiness of the professional competence of librarians. The method used in this research is a descriptive qualitative research method with data collection techniques of observation, interviews and documentation. In this study, researchers used data analysis techniques through three stages, namely data reduction, data presentation, and verification/drawing conclusions. To test the credibility of the data, triangulation technique was used. The result of this research is qualitative research. Data analysis is descriptive. The research subject is the iKuansing application, staff and librarians at Dispersip money. The object of this research is the readiness of the librarian's professional competence in the development and development of iKuansing. The results of this study are the iKuansing in the Dispersip Kuansing consisting of 10 indicators, according to the informant's perception 8 of the competencies already possessed by the librarian are: librarians have been able to create and manage efficient digital technology, librarians are able to monitor, filter, and select up-to-date, librarians have knowledge and management skills of Pusdokinfo, librarians are able to provide user education, librarians use information technology for information distribution, librarians able to develop information products that can be used anywhere, librarians are able to continuously update information services, librarians are able to understand the ethics of cyberspace already owned by librarians. Meanwhile, there are two indicators that do not have readiness, namely: librarians have not been able to assess the needs of users on a regular basis because librarians have not provided space for users to provide input and suggestions as evaluation material for librarians and libraries. Librarians have not been able to communicate information services to the leadership because iKuansing is an activity program that has been designed by the library planning department.
Reflexiones en torno a la violencia sexual: consecuencias y acciones
Authors: Rodriguez Narvaez Vanessa
Published: November 2022

La violencia sexual es un alarmante problema de salud pública que afecta a la sociedad peruana. Se revisa la situación de esta problemática en cuanto a su caracterización, incidencia en el país, y condiciones en las que se desarrolla esta problemática. Luego se revisa el impacto de esta problemática en la sociedad en su conjunto, así como en la salud mental. Posteriormente se analizan acciones importantes a considerar en el abordaje de la prevención y eliminación de violencia sexual, entendiendo que problemática requiere de una intervención multisectorial, considerando aspectos socioculturales, biosociológicos y jurídicos.
Big Data Enabled Non-Invasive Rapid Sex Detection of Incubated Chicken Eggs
Authors: Neethirajan Suresh
Published: November 2022

Current commercial, pre-commercial, and experimental in ovo techniques for the sex determination of fertilised eggs employ either minimally invasive biomolecular assays (extracting fluid via a small laser-drilled window in the eggshell, for detection of genetic or hormonal biomarkers), analysis of volatile compounds emitted from the eggshell, visible imaging, and reflectance or transmission spectroscopic analysis exploiting molecular optical fluorescence, polarisation, and scattering phenomena, including various combinations of these modalities. , to date no endeavour employing the NIR and FTIR based spectroscopic techniques has resulted in a commercially sustainable solution to the egg sexing problem. Besides achieving only subpar performance in overall accuracy, specificity, and sensitivity, the least invasive of the current state-of-the-art optical methods still requires, creating a transmission window (fenestration) of 12–15 mm diameter through to the mammillae layer of the shell, proximal to the external shell membrane, which can affect the incubation or post-hatch development viability of up to 10% of incubated eggs. Multimodal solution combining Raman spectroscopy and hyperspectral imaging has strong prospects to overcome the hard barriers existing before the perfection of a non-invasive in-line process for high reliability and rapid throughput for sex determination of eggs within 3 days of incubation. The method for sexing of chicken embryos needs to take a multipronged approach in collecting and analyzing spectral data that points to biomarkers using the machine learning approaches to look for nanomolar to picomolar concentrations of these in the fluid.
Identification and Pharmacological Activities of Secondary Metabolites of Phytochemicals
Authors: Ovonramwen Oluwaseyi Bukky
Published: November 2022

Plant secondary metabolites are organic compounds (alkaloids, flavonoids, phenolics, tannins, anthraquinones, saponins, steroids, lignins, terpenes) produced by plant for self-defense during growth and development in order to protect them from harmful agents. As population is increasing and quality of life is reducing couple with outbreak of some diseases that are posing threat on the economy, a lot of medicinal plants have attracted interest of researchers because of the usefulness of these metabolites in pharmaceuticals, food and cosmetic industries. In this review, the pharmacological activities of some medicinal plants that are rich in secondary metabolites were studied, means of identifying and quantifying using spectrophotometry and chromatography techniques were also discussed. However, this will assist to reduce the uses and dependent on the synthetic drug and the onset of the age related diseases.
Nutraceuticals in Personalized and Precision Medicine – a preliminary scoping review indicates efficacy for disease treatment, general wellbeing, and defense against Covid-19
Authors: Zahir Farah R.
Published: November 2022

Nutraceuticals have taken the spotlight during the past two decades as evidenced by the exponential publications on them. Long a part of routine in Traditional Medicine Systems, the rise of their mainstream use globally raises both safety concerns and need for better understanding of efficacious dosing. We attempt to answer these questions in this preliminary scoping review by an analysis of current literature on nutraceutical use as a personalized or prescription medicine. Using Covidence, Rayyan, and manual searches of PubMed, 598 unique publications were selected. 32 are systematic reviews, of which we overview the scope. We also overview 30 papers that address adverse drug reactions. To obtain an unbiased landscape of the 598 papers, we analyzed keywords using multiple methods. Expectedly, the most frequent keywords were probiotics and vitamins. Unexpectedly and remarkably, among the highest keyword yield was ‘COVID’. Further exploring this aspect, we review 15 pertinent papers, that not only provide robust evidence for nutraceutical benefits as part of SARS-CoV-2 treatment, but also amplify the notion that nutraceuticals are protective. Overall, the strident note is that further robust targeted research is needed in order to reap the full benefits of nutraceuticals in a safe and efficacious manner.
Epitope Mapping of Anti-mouse CCR3 Monoclonal Antibodies using Flow Cytometry
Authors: Tateyama Nami , Asano Teizo , Suzuki Hiroyuki , Li Guanjie , Yoshikawa Takeo , Tanaka Tomohiro , Kaneko Mika K. , Kato Yukinari
Published: November 2022

The CC chemokine receptor 3 (CCR3) is a receptor for CC chemokines, including CCL5/RANTES, CCL7/MCP-3, and CCL11/eotaxin. CCR3 is expressed on the surface of eosinophils, basophils, a subset of Th2 lymphocytes, mast cells, and airway epithelial cells. CCR3 and its ligands are involved in airway hyperresponsiveness in allergic asthma, ocular allergy, and cancers. Therefore, CCR3 is an attractive target for those therapies. Previously, anti-mouse CCR3 (mCCR3) monoclonal antibodies (mAbs), C3Mab-3 (rat IgG2a, kappa), and C3Mab-4 (rat IgG2a, kappa) were developed using the Cell-Based Immunization and Screening (CBIS) method. In this study, the binding epitope of these mAbs was investigated using flow cytometry. The CCR3 extracellular domain-substituted mutant analysis showed that C3Mab-3, C3Mab-4, and a commercially available mAb (J073E5) recognized the N-terminal region (amino acids 1–38) of mCCR3. Next, the alanine scanning was conducted in the N-terminal region. The results revealed that Ala2, Phe3, Asn4, and Thr5 of mCCR3 are involved in C3Mab-3 binding, whereas Ala2, Phe3, and Thr5 are essential to C3Mab-4 binding, and Ala2 and Phe3 are crucial to J073E5 binding. These results reveal the involvement of the N-terminus of mCCR3 in the recognition of C3Mab-3, C3Mab-4, and J073E5.
Condiciones sociales y de salud como determinantes de los contagios y fallecimientos por la covid-19 en México
Authors: Cuevas Rodríguez Enrique , Jaén Jiménez Bernardo , Castellanos Villarruel Maria Soledad
Published: November 2022

Desde que se detectó el primer caso de COVID-19 en el mundo y se transformó en pandemia, investigaciones científicas mostraron que los adultos de 60 años y más ha sido la población más afectada, más aún, si éstos sufren de diabetes, obesidad o hipertensión, entre otras comorbilidades. En esta línea que se inscribe este trabajo, que tuvo como objetivo medir el impacto de la densidad poblacional, nivel de pobreza y desigualdad en el número de contagios y fallecimientos. Los resultados confirmaron que, en la probabilidad de contagios, la variable más determinante fue la densidad de población, y en la de fallecimientos la edad. Las tres comorbilidades más significativas en la probabilidad de contagiarse fueron, en orden de importancia: la obesidad, tabaquismo y diabetes; y las tres más significativas en la probabilidad de fallecer: diabetes, obesidad y el tener una enfermedad renal crónica.
Распространение вариантов вируса SARS-COV-2, вызывающих озабоченность (VOC) на основе количества их геномов, депонированных в базу данных GISAID за неделю с 28.10.2022 г. по 03.11.2022 г.
Authors: Старшинов Василий , Краснов Ярослав , Чумачкова Елена , Осина Наталья , Зимирова Анастасия , Иванова Александра , Карнаухов Игорь , Караваева Татьяна , Щербакова Светлана , Кутырев Владимир
Published: November 2022

В обзоре представлена информация по циркулирующим в настоящее время вариантам вируса SARS-COV-2 вызывающих озабоченность (VOC), геномные последовательности которых размещены в международной базе данных GISAID за неделю с 28.10.2022 г. по 03.11.2022 г.
Анализ динамики эпидемического процесса COVID-19 в мире за неделю с 29.10.2022 г. по 03.11.2022 г.
Authors: Зимирова Анастасия , Поспелов Михаил , Иванова Александра , Дмитриева Людмила , Чумачкова Елена , Карнаухов Игорь , Щербакова Светлана , Кутырев Владимир
Published: November 2022

В обзоре представлен анализ эпидемиологической ситуации в странах мира по COVID-19, за неделю с 29.10.2022 г. по 03.11.2022 г.
Assessment of Posterolateral Lumbar Fusion
Authors: Lehr A. Mechteld , Duits Anneli A.A. , Reijnders Maarten R.L. , Nutzinger Don , Castelein René M. , Oner F. Cumhur , Kruyt Moyo C.
Published: November 2022

Background:

Noninvasive assessment of osseous fusion after spinal fusion surgery is essential for timely diagnosis of patients with symptomatic pseudarthrosis and for evaluation of the performance of spinal fusion procedures. There is, however, no consensus on the definition and assessment of successful posterolateral fusion (PLF) of the lumbar spine. This systematic review aimed to (1) summarize the criteria used for imaging-based fusion assessment after instrumented PLF and (2) evaluate their diagnostic accuracy and reliability.

Methods:

First, a search of the literature was conducted in November 2018 to identify reproducible criteria for imaging-based fusion assessment after primary instrumented PLF between T10 and S1 in adult patients, and to determine their frequency of use. A second search in July 2021 was directed at primary studies on the diagnostic accuracy (with surgical exploration as the reference) and/or reliability (interobserver and intraobserver agreement) of these criteria. Article selection and data extraction were performed by at least 2 reviewers independently. The methodological quality of validation studies was assessed with the QUADAS-2 (Quality Assessment of Diagnostic Accuracy Studies 2) and QAREL (Quality Appraisal of Reliability Studies).

Results:

Of the 187 articles included from the first search, 47% used a classification system and 63% used ≥1 descriptive criterion related to osseous bridging (104 articles), absence of motion (78 articles), and/or absence of static signs of nonunion (39 articles). A great variation in terminology, cutoff values, and assessed anatomical locations was observed. While the use of computed tomography (CT) increased over time, radiographs remained predominant. The second search yielded 11 articles with considerable variation in outcomes and quality concerns. Agreement between imaging-based assessment and surgical exploration with regard to demonstration of fusion ranged between 55% and 80%, while reliability ranged from poor to excellent.

Conclusions:

None of the available criteria for noninvasive assessment of fusion status after instrumented PLF were demonstrated to have both sufficient accuracy and reliability. Further elaboration and validation of a well-defined systematic CT-based assessment method that allows grading of the intertransverse and interfacet fusion mass at each side of each fusion level and includes signs of nonunion is recommended.

Level of Evidence:

Diagnostic Level III. See Instructions for Authors for a complete description of levels of evidence.
E-Commerce Information System Design in Web-Based UD Anggun Shoe Store
Authors: Akbar Billy Al Ghazian Ramadhan , Astutik Ika Ratna Indra
Published: November 2022

E-commerce or online shopping is a concept that is quite developed in the internet world. The use of this system can benefit many aspects, including consumers, producers, and sellers. The information system is a combination of work procedures, information, staffing, and information technology that is organized to achieve organizational goals. Information systems have become an urgent need in all areas of life including business. One ofthe companies engaged in trading, namely the Anggun Shoe Store, really needs information system technology that provides sales data recap functions and sales profit reports because consumers have difficulty getting detailed information on product specifications offered and employees find it difficult to offer products sold to consumers outside the city. Web-Based E-Commerce Information System Design (Anggun Shoe Store). An information system is needed by this company as well as other companies that run a similar business model, to facilitate sales reporting sothat their business can be measured. This system also makes it easier for business owners to view daily sales reports so they can determine marketing strategies for the future.
Evaluasi ketahanan galur melon madura (Cucumis melo L.) terhadap cucumber mosaic virus
Authors: Monica Eva , Khoiri Syaiful , Amzeri Achmad
Published: November 2022

Tanaman melon (Cucumis melo L.) merupakan salah satu tanaman buah-buahan yang penting dan tanaman melon ini banyak ditanam di berbagai negara. Permintaan dan produksi buah melon yang meningkat harus seimbang dengan ketersediaan benih melon. Dalam budidaya terdapat beberapa kendala salah satunya ketersediaan benih pada waktu yang dibutuhkan. Budidaya tanaman melon tidak terlepas dari hambatan pertumbuhan salah satunya karena gangguan hama dan penyakit. Mengatasi permasalahan produksi diperlukan perakitan varietas unggul yang tahan. Varietas unggul diperoleh dari tetua yang tahan. Sehingga penelitian ini bertujuan untuk mengevaluasi galur-galur melon lokal Madura yang memiliki ketahanan terhadap Cucumber mosaic virus (CMV). Penelitian dilakukan di greenhouse untuk penanaman melon, Program Studi Agroekoteknologi Fakultas Pertanian Universitas Trunojoyo Madura. Sampel daun yang bergejala penyakit dibawa ke laboratorium untuk dilakukan ekstrak genom. Amplifikasi DNA dilakukan dengan RT-PCR menggunakan sepasang primer CMV-1F CMV-1R. Hasil konfirmasi menunjukkan sampel positif terinfeksi CMV. Berdasarkan hasil percobaan secara umum galur G16 memiliki tingkat ketahanan terbaik dibandingkan galur lain dan galur pembanding. Selain itu, galur G8 juga cukup tahan dibandingkan galur pembanding. galur G16 dan galur G8 berpotensi digunakan sebagai tetua yang tahan CMV.
Plan de negocio de exportación de licor de arandanos al mercado de Estados Unidos
Authors:   , Anticona Cosar Yomira Katherine
Published: November 2022

El presente plan que realizamos costa de la creación de una nueva empresa exportadora con el objetivo de ofrecer a los clientes licor de arándanos ya que este fruto es muy abundante en nuestro país y se buscó la manera de poder llegar al mercado de EE. UU ya que se ve que existe una gran demanda por el consumo de la fruta y por la gran cantidad de preferencia en los licores que está a base de la fruta, por lo tanto, se planea exportar en el 2022 a dicho mercado. Para poder realizar nuestro plan se tomó en cuenta poder investigar el mercado norteamericano el cual estará desarrollado mediante herramientas como son el PESTEL y las 5 fuerzas de Porter que nos permiten conocer más a fondo nuestro mercado norteamericano ya que existe una gran competencia, así mismo vemos que es muy importante desarrollar nuestro FODA y el plan de negocio por el modelo CANVAS que nos permitirá llegar a dicho mercado. Como empresa planteamos realizar nuestro plan de marketing mix para poder fidelizar al cliente objetivo ofreciéndole un producto de calidad y que sientan la diferencia para que ellos mismos se den cuenta ya que se estará promocionando por diversas páginas web. En nuestro plan financiero que se tiene para poder poner en marcha nuestro negocio desarrollando una estructura que estará basada mediante la inversión de los cuatro socios que daremos un monto especifico de S/. 80,000 para poder llegar a cabo la empresa.
Diabetes Mellitus Tipo 2 y su asociación con características del sueño (duración, calidad y dificultad para dormir) en una población de Tumbes con edad entre 30 y 69 años
Authors:   , Merino Rivera Jimy Alex , Ruiz Burneo Cinthia Lucia
Published: November 2022

Objetivo: Determinar si existe asociación entre la presencia de diabetes mellitus tipo 2 (DM2) y ciertas características del sueño: duración, dificultad para dormir, y calidad, en una población de Tumbes entre 30 y 69 años de edad. Materiales y métodos: Estudio transversal analítico. Las variables dependientes fueron dificultad para dormir (a veces/casi nunca y con frecuencia), duración del sueño (normal, corto y prolongado) y calidad del sueño (buena y mala). La variable independiente fue la presencia de DM2 evaluada a través de la prueba de tolerancia oral a la glucosa (no DM2, con DM2 y sin diagnóstico previo, y con DM2 y con diagnóstico previo). Se usó modelos de regresión de Poisson para reportar razones de prevalencia (RP) e intervalos de confianza al 95% (IC95%). Resultados: Un total de 1607 individuos, edad media 48,2 años, 50,3% mujeres, fueron analizados; 71 (4,5%) tenían DM2 sin diagnóstico previo y 105 (6,5%) tenían DM2 con diagnóstico previo. De acuerdo a las características del sueño, 12,1% presentó sueño corto y un 8,2% presentó sueño prolongado, 3,7% reportó dificultad para dormir, y 19,5% tuvieron mala calidad de sueño. En modelo multivariable, tener DM2 estuvo asociado con dificultad para dormir (RP = 2,22; IC 95%: 1,14 - 4,30) y mala calidad de sueño (RP = 1,44; IC 95%: 1,05 – 1,97) en aquellos sujetos con diagnóstico previo. Conclusión: Los individuos con diagnóstico previo de DM2 tuvieron mayor probabilidad de presentar dificultad para dormir y mala calidad de sueño. Estos resultados sugieren la necesidad de evaluar periódicamente las características del sueño en pacientes con DM2.
Hipertensión arterial no diagnosticada en el Perú: análisis de factores asociados y de desigualdades, 2019
Authors:   , Montoya Rivera Wency Cecilia , Guerrero Díaz Delia Vanessa
Published: November 2022

Objetivo. Determinar los factores asociados y medir las desigualdades socioeconómicas en personas con hipertensión arterial (HTA) no diagnosticada en el Perú. Materiales y métodos. Estudio de tipo observacional, de corte transversal y analítico, en el que se utilizó la base de datos de la Encuesta Demográfica y de Salud Familiar (ENDES) del año 2019. La variable dependiente fue la presencia de HTA no diagnosticada (valores de presión arterial sistólica media ≥140 mmHg y/o presión arterial diastólica media ≥90 mmHg en las dos medidas de presión arterial y sin diagnóstico previo de HTA por parte de un profesional de la salud). Se estimaron razones de prevalencias ajustadas para identificar los factores asociados a la HTA no diagnosticada. La desigualdad socioeconómica en la población afectada por la HTA no diagnosticada se estimó con las curvas de concentración e índices de concentración de Erreygers. Resultados. De entre los 3697 pacientes con HTA, el 67,2% no había sido diagnosticado. La falta de diagnóstico de HTA fue más prevalente en hombres, residentes de la región costa y en habitantes a más de 3000 metros sobre el nivel del mar. Tener 50 años o más, contar con un seguro de salud, ser obeso y tener diabetes mellitus se asociaron a una menor prevalencia de HTA no diagnosticada. Se encontró una desigualdad en la falta de diagnóstico de HTA que se concentra en la población más pobre. Conclusiones. Al menos uno de cada dos peruanos adultos con HTA no ha sido diagnosticado de esta enfermedad. Se encontró desigualdad socioeconómica, así como factores sociodemográficos y relacionados al estado de salud asociados a la falta de diagnóstico de HTA. Nuestros hallazgos identifican algunos subgrupos de población en los que se deben priorizar las intervenciones para el tamizaje y el tratamiento de la HTA...
Burnout y síntomas ansioso-depresivos en trabajadores de salud de una clínica privada en tiempos de COVID-19
Authors:   , Ramirez Videla Gabriela Adriana , Jimenez Tornero Ximena
Published: November 2022

Objetivo: Determinar la asociación entre burnout y síntomas ansioso-depresivos en trabajadores de salud de una clínica en tiempos de covid-19. Métodos: Se realizó una investigación observacional, transversal en un universo de 220 trabajadores, de los cuales 177 conformaron la población de estudio. Se aplicó el cuestionario de Maslach para hallar la presencia de síndrome de Burnout, además se usó el cuestionario HADS para medir ansiedad y depresión. Se utilizó chi cuadrado para el análisis bivariado y regresión de poisson para el análisis multivariado entre variables. Resultados: La prevalencia de personal de salud con síndrome de Burnout (3.4%) y síntomas ansioso-depresivos (17,7%). La mediana de edad fue de 32 [28-38]años, el 68.3% eran mujeres y la mayoría de los participantes se encontraban solteros 57.7%. Se halló asociación estadísticamente significativa entre burnout y síntomas ansioso-depresivos (p<0.001). Conclusiones: Se halló asociación entre burnout y síntomas ansioso-depresivos. El personal de salud más afectado por el síndrome de burnout fue el médico y el más afectado por síntomas ansioso-depresivos fue el de enfermería.
Detección de DNA circulante en 24 pacientes con cáncer de mama triple negativo en el Instituto Nacional de Enfermedades Neoplásicas
Authors:   , De Olarte Guzman Briana Estefania , Angulo Nalvarte Jackeline Andrea
Published: November 2022

Objetivo. Determinar la presencia de ADN tumoral circulante y describir las características de mal pronóstico en pacientes con cáncer de mama triple negativo. Materiales y métodos. El presente estudio es de tipo descriptivo observacional y está basado en un análisis secundario de la base de datos obtenida en el INEN durante el periodo de los meses Julio 2016 y marzo 2017. La población del presente estudio fue de 24 pacientes de sexo femenino, mayores de 18 años diagnosticadas de Cáncer de mama triple negativo en el INEN. La variable dependiente fue cáncer de mama. La variable independiente fue DNA tumoral circulante. Resultados. Se analizaron 24 pacientes con CMTN, 7 de ellas presentaron mutación PI3KCA, la media de edad de diagnóstico fue de 45 años, el estadio clínico con mayor prevalencia fue el III y todas las pacientes presentaron un subtipo histológico ductal, 5 de ellas tuvieron recaída clínica y un 85% tuvo metástasis, ya sea cerebral, ganglionar, ósea o la más común, pulmonar, además se evidenció que todas las pacientes que presentaron recidivas a distancia fallecieron. Conclusiones. Gracias al presente estudio, podemos concluir que la detección de ADN-tc en pacientes con cáncer de mama triple negativo se puede realizar de manera satisfactoria y que la tasa de frecuencias de detección no es baja. Además, que la detección de ADN-tc puede indicar en algunos casos que el paciente presentará una corta sobrevida.
Asociación entre la expresión de CD44 y una menor sobrevida en una población peruana con cáncer de mama
Authors:   , Granda Oblitas Alexandra , Quiroz Gil Gema Ximena
Published: November 2022

En Perú, el cáncer de mama está catalogado como la segunda neoplasia maligna más frecuente. Se conoce que la tasa de supervivencia a los 5 años es baja, por lo cual hoy en día se busca identificar potenciales marcadores tumorales que pudieran contribuir al pronóstico. El CD44 es una glicoproteína transmembrana que se ha visto asociada a mayor carcinogénesis y aún se encuentra en estudio para identificar su relación con el cáncer de mama. Objetivo: Identificar la asociación entre la expresión de la proteína CD44 en las células tumorales y una menor sobrevida en pacientes con cáncer de mama. Material y métodos: Estudio observacional, analítico y retrospectivo en 101 pacientes mayores de edad, pertenecientes al Instituto Nacional de Enfermedades Neoplásicas (INEN) de Lima-Perú durante mayo 2016 a mayo 2018, con diagnóstico de cáncer de mama invasivo, las muestras fueron teñidas con CD44. Se analizó la base de datos mediante las pruebas Chi-cuadrado y la prueba p log rank con la curva de sobrevida Kaplan Meier. Resultados: CD44 fue positivo en el 76,2% de los casos, del cual se correlacionó con un menor estado de RE (p=0.040) y RP (p=0.023) en subtipo luminal A y una menor expresión de HER2 (P=0.04) y se relacionó con una sobrevida a los 3 años de 83,1%. Conclusiones: CD44 demostró un nivel de significancia al asociarlo a una sobrevida a los 3 años en pacientes con cáncer de mama, sin embargo, su rol permanece aún no concluyente y se requeriría de estudios más amplios.
Gestión del talento en una empresa de seguridad privada, Lima - 2021
Authors:   , Montauban Canga Giselle
Published: November 2022

El objetivo de la investigación fue identificar las etapas en la gestión del talento de una empresa mediana de seguridad privada en Lima Metropolitana durante el año 2021. El estudio tuvo un diseño no experimental, de corte descriptivo y tipo básico, tuvo un enfoque cuantitativo. Con un muestreo probabilístico la muestra quedó conformada por 62 sujetos de estudio. Se concluyó que la empresa cuenta con herramientas básicas para gestionar el talento, tanto en selección, capacitación y retención de este.
Comparación de la exactitud de cuatro localizadores apicales en premolares unirradiculares con simulación de reabsorción apical externa y reabsorción dentinaria interna
Authors:   , Lopez Maekawa Astrid Vanessa
Published: November 2022

Introducción: El objetivo del presente estudio in vitro fue el de comparar la exactitud de las mediciones de la longitud de trabajo de cuatro localizadores de foramen: Root ZX Mini, Raypex 6, Woodpex III y Propex Pixi en premolares unirradiculares con reabsorción radicular simuladas. Materiales y métodos: Para este estudio se utilizó 30 premolares permanentes unirradiculares. Las muestras se dividieron en dos grupos: 15 piezas dentarias con reabsorción dentinaria interna y 15 piezas dentarias con reabsorción externa simuladas. Las piezas dentarias fueron posicionadas en envases con alginato, cubriendo únicamente la porción radicular. Se realizaron las mediciones con los cuatro localizadores; y los resultados fueron comparados con las medidas de la longitud de trabajo obtenidas previamente con la técnica visual directa y el uso de un Microscópio usb digital 5x, aplicando para estas la prueba de Shapiro-Wilk. Las pruebas de Anova y T de Student para muestras relacionadas fueron ejecutadas para el análisis de las medidas obtenidas. Resultados: En las piezas dentarias con reabsorción apical externa no hubo diferencias estadísticamente significativas en las mediciones obtenidas con los localizadores Raypex 6 y Propex pixi, pero si hubo diferencias estadísticamente significativas con los localizadores de foramen Root zx mini (p=0.04) y Woodpex III (p=0.00). Por otro lado, en la muestra con reabsorción radicular interna no hubo diferencias estadísticamente significativas en las mediciones con los localizadores de foramen Propex pixi, Root zx mini y Raypex 6. Sin embargo, si hubo diferencias significativas con el Woodpex III. Conclusiones: Basado en este estudio in vitro, el localizador de foramen Raypex 6 fue el de mayor exactitud en premolares unirradiculares con reabsorción apical externa simulada y el Root zx mini fue el localizador más exacto en premolares con reabsorción dentinaria interna simulada...
The acidity of oxalic acid induces regulated cell death in Arabidopsis
Authors: Huang Guozhong , Liu Xiaotong , Zou Yanmin , Xiao Zejun , Chen Kexing , Cao Nana , Lu Dongping
Published: November 2022

Key message The acidity of oxalic acid (OA) induces an unknown type of cell death in Arabidopsis. Acid treatment and Sclerotinia sclerotiorum infection trigger some overlapping transcriptomic responses. The influx of calcium is likely required for OA-induced cell death in Arabidopsis.

Abstract Numerous pathogens trigger host cell death that either favors or blocks infection. Many phytopathogens produce cytolytic compounds functioning as virulence determinants, like oxalic acid (OA). It was reported that oxalate itself but not the acidity of OA induced programmed cell death in plants. Accumulation of oxalate often reaches millimolar concentrations, and the pH value decreases to around 4.0 in the Sclerotinia sclerotium -infected plant tissues. However, the role of OA acidity in inducing cell death remains unknown. We used the approaches of Sytox green/fluorescein diacetate (FDA)/propidium iodide (PI)/Evan's blue staining, transcriptomic analysis, and dynamic gene expression analysis to investigate the role of OA acidity in inducing cell death and the regulatory mechanisms of OA-induced cell death. We found that the acidity of OA and HCl induced cell death in Arabidopsis with characteristic features including impaired plasma membrane and induction of cell-death related genes, but not DNA fragmentation. Moreover, calcium influx is required for OA-induced cell death. Our work reveals that acids induce an uncharacterized-type of cell death that is a regulated process, instead of a passive event.
N6-methyladenosine-mediated changes in SOCS3 expression regulate skeletal muscle stem cell senescence
Authors: Zhu Menghai , Lian Chong , Chen Gang , Zou Peng , Qin Beng gang
Published: November 2022

N6-methyladenosine (m6A)-associated mechanisms are involved in cellular metabolic activities; however, their role in skeletal muscle stem cell (SkMSC) senescence is unknown. In this study, m6A RNA modification and METTL3 expression were decreased in aged SkMSCs from mice. Transcriptional analysis of m6A modification further revealed that suppressor of cytokine signaling 3 (SOCS3) was downregulated in aged SkMSCs, and that downregulation of METTL3 promoted SkMSC senescence. In addition, the upregulation of METTL3 alleviated the senescence of SkMSCs. Mechanistically, deletion of m6A modifications promoted senescence and inhibited SOCS3 expression, whereas downregulation of SOCS3 promoted senescence. Moreover, insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) was identified as an important regulator in the recognition and stabilization of m6A modified SOCS3 expression. IGF2BP1 binds to SOCS3 and reinforces its stability by suppressing the phosphorylation of JAK2/STAT3. Therefore, METTL3 inhibits SkMSC senescence through m6A modification-dependent stabilization of SOCS3 expression and inhibition of JAK2-STAT3 signaling. This study thus highlights a novel mechanism underlying SkMSC senescence.
Saffron-Fusarium oxysporum R1 dual transcriptomics unravels, defense mechanism of saffron and robust pathogenicity of Fusarium oxysporum R1
Authors: Bhagat Nancy , Mansotra Ritika , Patel Karan , vakhlu jyoti
Published: November 2022

Fusarium oxysporum causes corm rot in saffron (Crocus sativus L.), that is one of the most important fungal diseases impacting saffron yield globally. Despite the fact that the corm rot agent and its symptoms are widely known, little is known about the molecular basis of defense mechanism of saffron in response to Fusarium oxysporum infection. Therefore, the current study was initiated in order to identify differentially expressed genes in response to pathogen infection in saffron. The active participation of Mitogen Activated Kinase pathway (MAPK), Transcription factors (TFs), plant-hormone signalling, plant-pathogen interaction pathway and synthesis of PR proteins in defence of saffron against Fox R1 infection was revealed by Gene Ontology, KEGG pathway and MapMan analysis. In this study, the PR proteins had shown a robust antifungal activity. These findings revealed that the saffron has a powerful defense mechanism in the early stages of infection. In addition, fifty seven Fusarium oxysporum R1 genes linked to pathogenicity and virulence that expressed during the infection phase were also identified. Surprisingly, SIXgenes (secreted in the xylem) were not found in the current investigation, although these genes have been thoroughly described in other Fusarium oxysporum strains and are known to be one of the key virulence factors. Because saffron is a male sterile plant that can only be improved genetically by genome editing, this work will serve as a foundation for identifying genes that can be used to create saffron varieties resistant to Fox infection.
Observational study on the influence of an app-based self-management program on the quality of life of women with endometriosis.
Authors: Rohloff Nadine , Rothenhöfer Markus , Götz Teresa , Schäfer Sebastian
Published: November 2022

Purpose: Endometriosis can significantly impair the quality of life of those affected. Multimodal self-help measures are recommended but often difficult to access. Smartphone apps have been shown to improve quality of life for other conditions with chronic pain. The aim of this study was to examine whether there is evidence of beneficial effects of the smartphone app “Endo-App®” and whether a multicenter randomized controlled trial should be planned to substantiate these effects.
Methods: In a sample of N=106 women affected by endometriosis the present study determined the influence of the use of Endo-App ® on their quality of life. Among others, the validated questionnaire Endometriosis Health Profile from Oxford University was used for this purpose.
Results: The use of Endo-App ® lead to a highly significant improvement in quality of life already after two weeks. A statistically significant change was found for nine out of ten measured variables of quality of life. A series of further analyses validated that the measured positive effects were not due to other confounding factors.
Conclusion: In summary, the results indicate that the quality of life of women with endometriosis improved by the digital self-management tool Endo-App ®. More studies are needed to further explore the influence of the app on quality of life and as confirmatory evidence of beneficial effects. For this purpose, a randomized controlled trial should be conducted over a longer period of time.
Trial registration:
This trial is registered at clinicaltrials.gov under the registration number NCT05528601 on August 18, 2022. It was retrospectively registered.
Modification on nanocellulose extracted from kenaf (Hibiscus cannabinus) with 3-aminopropyltriethoxysilane for thermal stability in poly (vinyl alcohol) thin film composites
Authors: Taib Mohamad Nurul Azman Mohammad , Yee Tan Sue , Hussin M. Hazwan
Published: November 2022

Improvement in thermal stability of nanocellulose was done with silanization treatment, which started with the treatment of silylated kenaf nanocellulose (SKNC) using 3-aminopropyltriethoxysilane (APTES) in alcohol solution at 25°C with pH 4 within 4 hours. Then, SKNC with varied content from 0.5 to 2% (w/w) was reinforced in poly(vinyl alcohol) (PVA). The SKNC was characterized by FT-IR, XRD, TEM, and TGA. The peak at 1571 cm− 1 corresponding to N-H bending vibration was observed on SKNC spectra indicating a successful silane treatment follow-up by a reduction of peak 1642 and 1425 cm− 1, respectively. The crystallinity index for SKNC was increased to 70.34% as compared with KNC (61.73%). TEM showed a needle-like structure for both SKNC and KNC. The interaction between SKNC and PVA was characterized by FT-IR with an increment of peak 1089 and 1028 cm− 1, which shows the physical interaction that occurred between PVA and SKNC. The dispersion of SKNC was well homogenous as observed in FESEM. SKNC exhibited good thermal stability and a less amount of char was formed at 700°C compared to kenaf extractive free. Furthermore, TGA demonstrated improvement after addition of 2% SKNC into PVA as well as DSC increased the melting point of PVA.
SLC43A3 Promotes Oncogenesis and Predicts Poor Prognosis in Glioma
Authors: Li Jiaqi , Luan Xingzhao , Cen Yulin , Jiang Gen , Zhao Mingkuan , Xiang Wei , Zhou Jie
Published: November 2022

Purpose
Solute Carrier Family 43 Member 3 (SLC43A3) contributes to nucleobase transport in many metabolic diseases and is present as a fusion oncogene in some tumors. However, the potential clinical significance of SLC43A3 in the prognosis and progression of gliomas remains incompletely understood. The present study investigated the prognostic significance of SLC43A3 in glioma and the potential functional roles of SLC43A3 in this context.
Methods
A comprehensive analysis of two patient cohorts allowed us to establish a glioma prognosis model based on SLC43A3 expression. The biological processes related to SLC43A3 in glioma were investigated using functional enrichment analysis. Single-sample gene set enrichment analysis (ssGSEA) was used to evaluate the correlation between SLC43A3 and immune cell infiltration. Furthermore, SLC43A3 expression was detected in glioma patient samples, and functional analyses using siRNA knockdown were performed in U87-MG and U251 cell lines. Cell proliferation and apoptosis were measured using a cell counting kit-8 assay and flow cytometry. A transwell assay was used to assess cell migration.
Results
In glioma patients, high SLC43A3 expression negatively correlated with overall survival (OS) and progression-free survival (PFS), suggesting that the SLC43A3 expression level was an independent prognostic indicator that contributed to the pathogenesis of glioma, including tumor cell proliferation, migration, and apoptosis.
Conclusion
SLC43A3, an independent prognostic indicator, may be involved in the proliferation and migration of glioma cells and reduce the cell apoptosis to participate the carcinogenic pathway of glioma.
Knowledge, Attitude, and Practices of Physicians and Dentists on Medication-related Osteonecrosis of Jaw (MRONJ): A Cross-Sectional Survey
Authors: kazmi syed murtaza , Kaleem Humayun , Khan Farhan , Khan Shahrukh
Published: November 2022

Background: Bisphosphonate medication can cause osteonecrosis of the jaw, which is an uncommon but serious complication. The exact mechanism for the development of MRONJ is not known clearly. Dentoalveolar surgery is considered the major risk factor as a local risk factor with pre-existing inflammatory dental diseases, such as periodontal disease or periapical pathology. Therefore, this survey aims to explore the cross-reference of the patients before prescribing anti-resorptive and related medicines to avoid medication-related osteonecrosis of the jaw (MRONJ) by evaluating and managing any potential dental issues and do the required surgeries and uplift their oral hygiene before the commencement of such medications.
Methods: An observational cross-sectional study was conducted among physicians and dentists of secondary and tertiary care hospitals and clinics in Pakistan between March and June 2021. Data were collected through a web-based questionnaire distributed among the eligible sample of physicians and dentists in the departments involved in prescribing bisphosphonates and managing the Osteonecrosis. SPSS 23.0 was used for data analysis. Frequencies and percentages were reported for all variables while Chi-squares and Fischer’s exact test was used to see differences in responses among physicians and dentists. A p-value of <0.05 was taken as statistically significant.
Results: A response rate of 29% was achieved. All dentists answered correctly when asked about the indications of bisphosphonates but only about half of them agreed that bisphosphonates can cause but have limited knowledge related to other medicines like anti-angiogenic and mTOR inhibitors. 50% of the physicians provide patient education regarding the potential adverse effect of bisphosphonate medicine and related drugs while others don’t educate the patient regularly.
Conclusions: Our study revealed a deficient knowledge regarding bisphosphonate-related ONJ among physicians and dentists. Appropriate training measures are required to raise their awareness.
Spontaneous portosystemic shunt as a potiential marker for severe liver damage and high risk of complications associated with portal hypertension in patients with hepatitis B-related cirrhosis: a multicenter study from China
Authors: Ke Qiao , Yu Xueping , Wang Xuan , Huang Jinfa , Lin Biding , Wang Zhiyong , Huang Xinhui , Weng Xiadi , Jiao Yubing , Xie Yixing , Wu Weimin , Qiu Linbin , Zheng Hanghai , Li Ling , Xu Zhengju , Su Zhijun , Zhuge Yuzheng , Liu Jingfeng , Guo Wuhua
Published: November 2022

Background and aim Patients with cirrhosis have a high prevalence of spontaneous portosystemic shunt (SPSS), but it remains controversial whether the presence of SPSS is associated with liver function and portal hypertension (PHT)-related complications. In this study, we aimed to investigate the prevalence, clinical characteristics and related factors of SPSS in cirrhotic patients. Methods Patients who were diagnosed with hepatitis B-related cirrhosis between Jan 2020 and Oct 2021 were retrospectively recruited from five centers in China. All eligible patients were classified into SPSS and non-SPSS groups and their clinical characteristics were compared. Logistic regression analyses were performed to identify clinical characteristics associated with SPSS, and then to assess the independent impact of SPSS on the risk of PHT-related complications. Results Of the 1282 patients included in this study, SPSS was identified in 488 patients (38.1%). SPSS group had a higher proportion of patients with hepatofugal flow in portal vein, thinner diameter of right branch of portal vein (RPV), thicker diameter of left branch of portal vein (LPV), splenic vein (SV) and superior mesenteric vein (SMV), more severe liver function impairment, higher incidence and severity of esophageal and gastric varices (EGV), and a higher prevalence of PHT-related complications [EGV bleeding (EGVB), portal vein thrombosis (PVT), hepatic encephalopathy (HE), ascites, and hepatocellular carcinoma (HCC)] (allP < 0.05). On multivariable logistic regression analyses, MELD score, diameter of RPV and SV, hepatofugal flow in portal vein, EV or GV or EGV on radiological evaluation, presence of EGVB, PVT, HE, and moderate–severe ascites were independently associated with SPSS (allP < 0.05). In addition, presence of SPSS was identified as an independent risk factor for EGVB, PVT and HE (allP < 0.05). Conclusion SPSS may indicate severe liver damage and a high risk of PHT-related complications.
360 Health Analysis (H360) – A comparison of key performance indicators in breast cancer management across health institution settings
Authors: Rego Inês Brandão , Coelho Sara , Semedo Patrícia Miguel , Cavaco-Silva Joana , Teixeira Laetitia , Sousa Susana , Reis Joana , Dinis Rui , Schmitt Fernando , Afonso Noémia , Fougo José Luís , Pavão Francisco , Leite Ricardo Baptista , Costa Luís
Published: November 2022

Background
The increased focus on quality indicators (QIs) and the use of clinical registries in real-world cancer studies have led to higher compliance with therapeutic standards and increased patient survival. The European Society of Breast Cancer Specialists (EUSOMA) defined QIs to assess compliance with current standards in breast cancer care.
Methods
This population-based retrospective study is part of the H360 Health Analysis (H360) project - Phase 2 and represents the first comprehensive assessment of the quality of breast cancer care in different hospital settings in Portugal. Its aim is to describe compliance with EUSOMA-defined QIs in breast cancer management in different hospital settings (public vs. private; general hospitals vs. Oncology centers) and thereby assess equity in the provision of care across health institutions. To do so, a set of key performance indicators (KPIs) was selected based on EUSOMA and previously identified QIs. Secondary data were retrieved from patients’ clinical records. Compliance with target KPIs in different stages of disease across the considered hospital settings was compared with minimum and target EUSOMA standards.
Results
A total of 259 patient clinical records were assessed. In stage I, II, and III disease, eighteen KPIs met target EUSOMA standards, five met minimum standards, and eight failed to meet minimum standards. Compliance with KPIs varied according to the type of hospital (particularly concerning diagnosis) and stage of the disease.
Conclusions
Study results show that, although most QIs meet EUSOMA standards, there is room for improvement regarding diagnostic and therapeutic procedures. There are differences in the clinical practice across health institutions, particularly between Oncology centers and general hospitals regarding diagnosis and KPI compliance among disease stages. These findings should be addressed in future studies in a coordinated effort to improve the quality of breast cancer care nationally.