bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Review of the Influence of Internal Marketing on Service Innovation
Authors: Raeisi Soheila , Suhaili Ramli Nur , Lingjie Meng
Published: September 2020

This paper aims aimed to present the trends of the literature review in internal marketing and service innovation between 1990 and 2016. The significant reason to conduct this research is that significant variables of internal marketing to link with service innovation are not clearly defined. This research yielded 22 systematic reviews of articles in the Scopus library and adopted a thematic analysis to analyze the data collected. This study provides provided answers to research questions by elaborating on overall trends, objectives, theoretical framework, methodologies, and potential variables that strongly connect between internal marketing and service innovation. While sample sizes are limited to this paper, it suggests suggested fruitful recommendations for future research to overcome this limitation. This research has had two practical implications for managers to redefine their roles and the relationship between members of the organization and to help managers and the firms to consider internal marketing efforts towards motivation, organizational culture, and organizational learning.
Novel Linguistic Steganography Based on Character-Level Text Generation
Authors: Xiang Lingyun , Yang Shuanghui , Liu Yuhang , Li Qian , Zhu Chengzhang
Published: September 2020

With the development of natural language processing, linguistic steganography has become a research hotspot in the field of information security. However, most existing linguistic steganographic methods may suffer from the low embedding capacity problem. Therefore, this paper proposes a character-level linguistic steganographic method (CLLS) to embed the secret information into characters instead of words by employing a long short-term memory (LSTM) based language model. First, the proposed method utilizes the LSTM model and large-scale corpus to construct and train a character-level text generation model. Through training, the best evaluated model is obtained as the prediction model of generating stego text. Then, we use the secret information as the control information to select the right character from predictions of the trained character-level text generation model. Thus, the secret information is hidden in the generated text as the predicted characters having different prediction probability values can be encoded into different secret bit values. For the same secret information, the generated stego texts vary with the starting strings of the text generation model, so we design a selection strategy to find the highest quality stego text from a number of candidate stego texts as the final stego text by changing the starting strings. The experimental results demonstrate that compared with other similar methods, the proposed method has the fastest running speed and highest embedding capacity. Moreover, extensive experiments are conducted to verify the effect of the number of candidate stego texts on the quality of the final stego text. The experimental results show that the quality of the final stego text increases with the number of candidate stego texts increasing, but the growth rate of the quality will slow down.
On a Free Boundary Problem for American Options Under the Generalized Black–Scholes Model
Authors: Lee Jung-Kyung
Published: September 2020

We consider the problem of pricing American options using the generalized Black–Scholes model. The generalized Black–Scholes model is a modified form of the standard Black–Scholes model with the effect of interest and consumption rates. In general, because the American option problem does not have an exact closed-form solution, some type of approximation is required. A simple numerical method for pricing American put options under the generalized Black–Scholes model is presented. The proposed method corresponds to a free boundary (also called an optimal exercise boundary) problem for a partial differential equation. We use a transformed function that has Lipschitz character near the optimal exercise boundary to determine the optimal exercise boundary. Numerical results indicating the performance of the proposed method are examined. Several numerical results are also presented that illustrate a comparison between our proposed method and others.
Possibilities of Using Kalman Filters in Indoor Localization
Authors: Fronckova Katerina , Prazak Pavel
Published: September 2020

Kalman filters are a set of algorithms based on the idea of a filter described by Rudolf Emil Kalman in 1960. Kalman filters are used in various application domains, including localization, object tracking, and navigation. The text provides an overview and discussion of the possibilities of using Kalman filters in indoor localization. The problems of static localization and localization of dynamically moving objects are investigated, and corresponding stochastic models are created. Three algorithms for static localization and one algorithm for dynamic localization are described and demonstrated. All algorithms are implemented in the MATLAB software, and then their performance is tested on Bluetooth Low Energy data from a real indoor environment. The results show that by using Kalman filters, the mean localization error of two meters can be achieved, which is one meter less than in the case of using the standard fingerprinting technique. In general, the presented principles of Kalman filters are applicable in connection with various technologies and data of various nature.
Success History-Based Adaptive Differential Evolution Using Turning-Based Mutation
Authors: Sun Xingping , Jiang Linsheng , Shen Yong , Kang Hongwei , Chen Qingyi
Published: September 2020

Single objective optimization algorithms are the foundation of establishing more complex methods, like constrained optimization, niching and multi-objective algorithms. Therefore, improvements to single objective optimization algorithms are important because they can impact other domains as well. This paper proposes a method using turning-based mutation that is aimed to solve the problem of premature convergence of algorithms based on SHADE (Success-History based Adaptive Differential Evolution) in high dimensional search space. The proposed method is tested on the Single Objective Bound Constrained Numerical Optimization (CEC2020) benchmark sets in 5, 10, 15, and 20 dimensions for all SHADE, L-SHADE, and jSO algorithms. The effectiveness of the method is verified by population diversity measure and population clustering analysis. In addition, the new versions (Tb-SHADE, TbL-SHADE and Tb-jSO) using the proposed turning-based mutation get apparently better optimization results than the original algorithms (SHADE, L-SHADE, and jSO) as well as the advanced DISH and the jDE100 algorithms in 10, 15, and 20 dimensional functions, but only have advantages compared with the advanced j2020 algorithm in 5 dimensional functions.
On the Beckenbach–Gini–Lehmer Means and Means Mappings
Authors: Matkowski Janusz , Wróbel Małgorzata
Published: September 2020

Beckenbacg–Gini–Lehmer type means and mean-type mappings generated by functions of several variables, for which the arithmetic mean is invariant, are introduced. Equality of means of that type, their homogeneity, and convergence of the iterates of the respective mean-type mappings are considered. An application to solving a functional equation is given.
Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report
Authors: Aprea Giuseppe , Scattolini Silvia , D’Angelantonio Daniela , Chiaverini Alexandra , Di Lollo Valeria , Olivieri Sabrina , Marcacci Maurilia , Mangone Iolanda , Salucci Stefania , Antoci Salvatore , Cammà Cesare , Di Pasquale Adriano , Migliorati Giacomo , Pomilio Francesco
Published: September 2020

Hepatitis E virus (HEV) is an emergent zoonotic pathogen, causing worldwide acute and chronic hepatitis in humans. HEV comprises eight genotypes and several subtypes. HEV genotypes 3 and 4 (HEV3 and HEV4) are zoonotic. In Italy, the most part of HEV infections (80%) is due to autochthonous HEV3 circulation of the virus, and the key role played by wild animals is generally accepted. Abruzzo is an Italian region officially considered an HEV “hot spot”, with subtype HEV3-c being up to now the only one reported among wild boars. During the year 2018–2019, a group of wild boars in Abruzzo were screened for HEV; positive RNA liver samples were subjected to HEV characterization by using the whole genome sequencing (WGS) approach methodology. This represents the first report about the detection of HEV-3 subtypes e and f in the wild boar population in this area. Since in Italy human infections from HEV 3-e and f have been associated with pork meat consumption, our findings deserve more in-depth analysis with the aim of evaluating any potential correlation between wild animals, the pork chain production and HEV human infections.
Diversity and Abundance of Microbial Communities in UASB Reactors during Methane Production from Hydrolyzed Wheat Straw and Lucerne
Authors: Liu Tong , Schnürer Anna , Björkmalm Johanna , Willquist Karin , Kreuger Emma
Published: September 2020

The use of straw for biofuel production is encouraged by the European Union. A previous study showed the feasibility of producing biomethane in upflow anaerobic sludge blanket (UASB) reactors using hydrolyzed, steam-pretreated wheat straw, before and after dark fermentation with Caldicellulosiruptor saccharolyticus, and lucerne. This study provides information on overall microbial community development in those UASB processes and changes related to acidification. The bacterial and archaeal community in granular samples was analyzed using high-throughput amplicon sequencing. Anaerobic digestion model no. 1 (ADM1) was used to predict the abundance of microbial functional groups. The sequencing results showed decreased richness and diversity in the microbial community, and decreased relative abundance of bacteria in relation to archaea, after process acidification. Canonical correspondence analysis showed significant negative correlations between the concentration of organic acids and three phyla, and positive correlations with seven phyla. Organic loading rate and total COD fed also showed significant correlations with microbial community structure, which changed over time. ADM1 predicted a decrease in acetate degraders after a decrease to pH ≤ 6.5. Acidification had a sustained effect on the microbial community and process performance.
A Genome-Scale Metabolic Model of Thalassiosira pseudonana CCMP 1335 for a Systems-Level Understanding of Its Metabolism and Biotechnological Potential
Authors: Ahmad Ahmad , Tiwari Archana , Srivastava Shireesh
Published: September 2020

Thalassiosira pseudonana is a transformable and biotechnologically promising model diatom with an ability to synthesise nutraceuticals such as fucoxanthin and store a significant amount of polyglucans and lipids including omega-3 fatty acids. While it was the first diatom to be sequenced, a systems-level analysis of its metabolism has not been done yet. This work presents first comprehensive, compartmentalized, and functional genome-scale metabolic model of the marine diatom Thalassiosira pseudonana CCMP 1335, which we have termed iThaps987. The model includes 987 genes, 2477 reactions, and 2456 metabolites. Comparison with the model of another diatom Phaeodactylum tricornutum revealed presence of 183 unique enzymes (belonging primarily to amino acid, carbohydrate, and lipid metabolism) in iThaps987. Model simulations showed a typical C3-type photosynthetic carbon fixation and suggested a preference of violaxanthin–diadinoxanthin pathway over violaxanthin–neoxanthin pathway for the production of fucoxanthin. Linear electron flow was found be active and cyclic electron flow was inactive under normal phototrophic conditions (unlike green algae and plants), validating the model predictions with previous reports. Investigation of the model for the potential of Thalassiosira pseudonana CCMP 1335 to produce other industrially useful compounds suggest iso-butanol as a foreign compound that can be synthesized by a single-gene addition. This work provides novel insights about the metabolism and potential of the organism and will be helpful to further investigate its metabolism and devise metabolic engineering strategies for the production of various compounds.
Advances in Antibody Preparation Techniques for Immunoassays of Total Aflatoxin in Food
Authors: Wang Yanan , Jiang Jinqing , Fotina Hanna , Zhang Haitang , Chen Junjie
Published: September 2020

Aflatoxin (AF) contamination is a major concern in the food and feed industry because of its prevalence and toxicity. Improved aflatoxin detection methods are still needed. Immunoassays are an important method for total aflatoxin (TAF) analysis in food due to its technical advantages such as high specificity, sensitivity, and simplicity, but require high-quality antibodies. Here, we first review the three ways to prepare high-quality antibodies for TAF immunoassay, second, compare the advantages and disadvantages of antigen synthesis methods for B-group and G-group aflatoxins, and third, describe the status of novel genetic engineering antibodies. This review can provide new methods and ideas for the development of TAF immunoassays.
Synthesis and Characterization of Magnetic Nanomaterials with Adsorptive Properties of Arsenic Ions
Authors: Wojciechowska Agnieszka , Lendzion-Bieluń Zofia
Published: September 2020

A new synthesis method of hybrid Fe3O4/C/TiO2 structures was developed using microwave-assisted coprecipitation. The aim of the study was to examine the effect of the addition of glucose and titanium dioxide on adsorptive properties enabling removal of arsenic ions from the solution. The study involved the synthesis of pure magnetite, magnetite modified with glucose and magnetite modified with glucose and titanium dioxide in magnetite: glucose: titanium dioxide molar ratio 1:0.2:3. Materials were characterized by XRD, FT-IR, and BET methods. Magnetite and titanium dioxide nanoparticles were below 20 nm in size in obtained structures. The specific surface area of pure magnetite was approximately 79 m2/g while that of magnetite modified with titanium dioxide was above 190 m2/g. Obtained materials were examined as adsorbents used for removal As(V) ions from aqueous solutions. Adsorption of arsenic ions by pure magnetite and magnetite modified with titanium dioxide was very high, above 90% (initial concentration 10 mg/L), pH in the range from 2 to 7. The preparation of magnetic adsorbents with a high adsorption capacity of As(V) ions was developed (in the range from 19.34 to 11.83 mg/g). Magnetic properties enable the easy separation of an adsorbent from a solution, following adsorption.
An Insight into the Excitation States of Small Molecular Semiconductor Y6
Authors: Zou Xianshao , Wen Guanzhao , Hu Rong , Dong Geng , Zhang Chengyun , Zhang Wei , Huang Hao , Dang Wei
Published: September 2020

Y6 is a new type of non-fullerene acceptor, which has led to power conversion efficiencies of single-junction polymer solar cells over 17% when combined with a careful choice of polymeric donors. However, the excited state characteristics of Y6, which is closely correlated with its opto-electronic applications, are not clear yet. In this work, we studied the excited state properties of the Y6 solution and Y6 film, by using steady-state and time-resolved spectroscopies as well as time-dependent density functional theory (TD-DFT) calculations. UV-Vis absorption and fluorescence simulation, natural transition orbitals (NTOs) and hole-electron distribution analysis of Y6 solution were performed for understanding the excitation properties of Y6 by using TD-DFT calculations. The lifetimes of the lowest singlet excited state in Y6 solution and film were estimated to be 0.98 and 0.8 ns, respectively. Combining the exciton lifetime and photoluminescence (PL) quantum yield, the intrinsic radiative decay lifetimes of Y6 in the solution and film were estimated, which were 1.3 and 10.5 ns for the Y6 solution and film, respectively. Long exciton lifetime (~0.8 ns) and intrinsic radiative decay lifetime (~10.5 ns) of Y6 film enable Y6 to be a good acceptor material for the application of polymer solar cells.
Characterization of Volatile Organic Compounds of Healthy and Huanglongbing-Infected Navel Orange and Pomelo Leaves by HS-GC-IMS
Authors: Cao Shan , Sun Jingyu , Yuan Xiaoyong , Deng Weihui , Zhong Balian , Chun Jiong
Published: September 2020

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the only natural vector of bacteria responsible for Huanglongbing (HLB), a worldwide destructive disease of citrus. ACP reproduces and develops only on the young leaves of its rutaceous host plants. Olfactory stimuli emitted by young leaves may play an important role in ACP control and HLB detection. In this study, volatile organic compounds (VOCs) from healthy and HLB-infected young leaves of navel orange and pomelo were analyzed by headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS). A total of 36 compounds (including dimers or polymers) were identified and quantified from orange and 10 from pomelo leaves. Some compounds showed significant differences in signal intensity between healthy and HLB-infected leaves and may constitute possible indicators for HLB infection. Principal component analysis (PCA) clearly discriminated healthy and HLB-infected leaves in both orange and pomelo. HS-GC-IMS was an effective method to identify VOCs from leaves. This study may help develop new methods for detection of HLB or find new attractants or repellents of ACP for prevention of HLB.
LC-MS-Based Metabolomics for the Chemosystematics of Kenyan Dodonaea viscosa Jacq (Sapindaceae) Populations
Authors: Kaigongi Magrate M. , Lukhoba Catherine W. , Ochieng‘ Purity J. , Taylor Malcolm , Yenesew Abiy , Makunga Nokwanda P.
Published: September 2020

Dodonaea viscosa Jacq (Sapindaceae) is a medicinal plant with a worldwide distribution. The species has undergone enormous taxonomic changes which caused confusion amongst plant users. In Kenya, for example, two varieties are known to exist based on morphology, i.e., D. viscosa var. viscosa along the coast, and D. viscosa var. angustifolia in the Kenyan inland. These two taxa are recognized as distinct species in some reports. This prompted us to apply metabolomics to understand the relationship among naturally occurring populations of D. viscosa in Kenya, and to identify compounds that can assist in taxonomic delineation of the different varieties of D. viscosa from different parts of Kenya. The phytochemical variability of Kenyan D. viscosa var. angustifolia populations collected from four different geographical regions (Nanyuki, Machakos, Nairobi, and Narok) and one coastal D. viscosa var. viscosa (the Gazi) were analyzed by LC-MS using a metabolomics-driven approach. Four known compounds, two diterpenoids (dodonic acid (1), hautriwaic acid lactone (3), and two flavonoids (5,7,4′,5′-tetrahydroxy-3,6,2′-trimethoxyflavone (2) and catechin (4)) were isolated and purified from the Gazi coastal collection. The presence of these compounds and their relative abundance in other populations was determined by LC-MS analyses. Multivariate statistical analyses of LC-MS data was used for the visualization of the patterns of variation and identification of additional compounds. Eleven discriminant compounds responsible for separating chemometric clusters were tentatively identified. In an antimicrobial assay, hautriwaic acid lactone (3) and catechin (4) were the most active compounds followed by the extract from the coastal (Gazi) population. The clustering pattern of the five populations of D. viscosa suggested that the metabolite profiles were influenced by geo-environmental conditions and did not support the current classification of D. viscosa based on morphology. This study disputes the current classification of D. viscosa in Kenya and recommends revision using tools such as molecular phylogenetics.
The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
Authors: Yang Hui , Heyer Jasmine , Zhao Hui , Liang Shengxian , Guo Rui , Zhong Li
Published: September 2020

(1) Background: Cathepsin K has been found overexpressed in several malignant tumors. However, there is little information regarding the involvement of Cathepsin K in non-small cell lung cancer (NSCLC). (2) Methods: Cathepsin K expression was tested in human NSCLC cell lines A549 and human embryo lung fibroblast MRC-5 cells using Western blot and immunofluorescence assay. Cathepsin K was transiently overexpressed or knocked down using transfection with a recombinant plasmid and siRNA, respectively, to test the effects on cell proliferation, migration, invasion, and on the mammalian target of rapamycin (mTOR) signaling pathway. (3) Results: Expression of Cathepsin K was increased significantly in A549 cells and diffused within the cytoplasm compared to the MRC-5 cells used as control. Cathepsin K overexpression promoted the proliferation, migration, and invasion of A549 cells, accompanied by mTOR activation. Cathepsin K knockdown reversed the above malignant behavior and inhibited the mTOR signaling activation, suggesting that Cathepsin K may promote the progression of NSCLC by activating the mTOR signaling pathway. (4) Conclusion: Cathepsin K may potentially represent a viable drug target for NSCLC treatment.
Vibrational Spectroscopy Saliva Profiling as Biometric Tool for Disease Diagnostics: A Systematic Literature Review
Authors: Derruau Stéphane , Robinet Julien , Untereiner Valérie , Piot Olivier , Sockalingum Ganesh D. , Lorimier Sandrine
Published: September 2020

Saliva is a biofluid that can be considered as a “mirror” reflecting our body’s health status. Vibrational spectroscopy, Raman and infrared, can provide a detailed salivary fingerprint that can be used for disease biomarker discovery. We propose a systematic literature review based on the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines to evaluate the potential of vibrational spectroscopy to diagnose oral and general diseases using saliva as a biological specimen. Literature searches were recently conducted in May 2020 through MEDLINE-PubMed and Scopus databases, without date limitation. Finally, over a period of 10 years, 18 publications were included reporting on 10 diseases (three oral and seven general diseases), with very high diagnostic performance rates in terms of sensitivity, specificity, and accuracy. Thirteen articles were related to six different cancers of the following anatomical sites: mouth, nasopharynx, lung, esophagus, stomach, and breast. The other diseases investigated and included in this review were periodontitis, Sjögren’s syndrome, diabetes, and myocardial infarction. Moreover, most articles focused on Raman spectroscopy (n = 16/18) and more specifically surface-enhanced Raman spectroscopy (n = 12/18). Interestingly, vibrational spectroscopy appears promising as a rapid, label-free, and non-invasive diagnostic salivary biometric tool. Furthermore, it could be adapted to investigate subclinical diseases—even if developmental studies are required.
Using Medicinal Plants in Valmalenco (Italian Alps): From Tradition to Scientific Approaches
Authors: Bottoni Martina , Milani Fabrizia , Colombo Lorenzo , Nallio Kevin , Colombo Paola Sira , Giuliani Claudia , Bruschi Piero , Fico Gelsomina
Published: September 2020

This ethnobotanical survey was carried out in Caspoggio (Valmalenco, SO, Italy) with the purpose of investigating the traditional uses of medicinal plants. Moreover, a bibliographic research meant to validate or refute the uses, focusing on the potentially responsible compounds, was performed. Fifty-nine species, attributable to 30 families (Asteraceae, Pinaceae, Malvaceae, and Lamiaceae the most cited), were mentioned. Arnica montana, anti-inflammatory for traumas and musculoskeletal pains; Pinus mugo, expectorant; Malva sylvestris, anti-inflammatory and soothing; Achillea moschata, digestive. The compounds, responsible for the therapeutic activities, are often polyphenols and terpenoids: helenanin in A. montana, α-pinene, δ-3-carene, and limonene in P. mugo, gossypin and malvin in M. sylvestris, luteolin and apigenin in A. moschata. Scientific evidence for at least one of the traditional activities described was found for 50 species but only in 26 out of 196 works consulted, it is possible to make a comparison between investigated extracts and traditional preparations. This study is thus a stimulus to new phytochemical investigations, mimicking as much as possible the traditional preparations. This work is part of the European Interreg Italy-Switzerland B-ICE project, aimed at creating a management model for the ongoing climate change and searching for new sources of territory valorization as attractions for tourists.
Effects of Omega-3 Polyunsaturated Fatty Acid Supplementation on Non-Alcoholic Fatty Liver: A Systematic Review and Meta-Analysis
Authors: Lee Cheng-Han , Fu Yun , Yang Shih-Jyun , Chi Ching-Chi
Published: September 2020

(1) Aim: Non-alcoholic fatty liver disease (NAFLD) is a prevalent disease worldwide. Omega-3 polyunsaturated fatty acids (n-3 PUFAs) bear anti-inflammatory action and can ameliorate hyperlipidemia. We wish to appraise the effects of n-3 PUFAs supplement on NAFLD. (2) Methods: We searched CENTRAL, Embase, and MEDLINE on 29 March 2020 for randomized control trials (RCTs) on the effects of n-3 PUFAs supplementation in treating NAFLD. The Cochrane Collaboration’s tool was used to assess the risk of bias of included RCTs. (3) Results: We included 22 RCTs with 1366 participants. The risk of bias of included RCTs was generally low or unclear. n-3 PUFAs supplementation significantly reduced liver fat compared with placebo (pooled risk ratio 1.52; 95% confidence interval (CI) 1.09 to 2.13). n-3 PUFAs supplementation also significantly improved the levels of triglyceride, total cholesterol, high-density lipoprotein, and body-mass index, with pooled mean difference and 95% CI being −28.57 (−40.81 to −16.33), −7.82 (−14.86 to −0.79), 3.55 (1.38 to 5.73), and −0.46 (−0.84 to −0.08), respectively. (4) Conclusions: The current evidence supports the effects of n-3 PUFAs supplementation in improving fatty liver. n-3 PUFAs supplementation may also improve blood lipid levels and obesity.
An Assessment of Three Carbohydrate Metrics of Nutritional Quality for Packaged Foods and Beverages in Australia and Southeast Asia
Authors: Tan Denise , Olden Andrea , Orengo Audrey , Francey Célia , Campos Vanessa , Fayet-Moore Flavia , Kim Jung , Lê Kim-Anne
Published: September 2020

Carbohydrate quality is an aetiological factor of diet-related disease. Indices of carbohydrate quality featuring various ratios of carbohydrates-to-dietary fibre-to-sugar have been associated with improved product and/or diet quality in westernised countries. Carbohydrate intake is especially high in Asia Pacific. Thus, this study evaluated the ability of such carbohydrate metrics to discriminate the nutritional quality of carbohydrate-rich packaged foods and beverages in Australia, Malaysia, Singapore, Thailand and the Philippines, with an additional focus on beverages. This evaluation was conducted by comparing product nutritional composition and assessing products against three national nutrient profiling models. Results showed that Australia had the highest proportion of products meeting all metrics, compared to the Southeast Asian countries. Beverages had a low adherence to all metrics compared to solid foods. Across the five countries, both processed food and beverages meeting the metrics generally contained higher dietary fibre, protein, and certain vitamins and minerals whilst having lower energy, total sugars, free sugars, trans fat and cholesterol content compared to products not meeting the metrics. The metrics were also aligned with national nutrient profiling models to identify nutritious products. In conclusion, these metrics allowed us to discriminate product nutritional quality in the countries assessed and are applicable to beverages.
25-Hydroxycholecalciferol Concentration Is Associated with Protein Loss and Serum Albumin Level during the Acute Phase of Burn Injury
Authors: Krajewski Andrzej , Piorun Krzysztof , Maciejewska-Markiewicz Dominika , Markowska Marta , Skonieczna-Żydecka Karolina , Stachowska Ewa , Polakowska Zofia , Mazurek Maciej , Szczuko Małgorzata
Published: September 2020

Background: Burned patients have an increased need for vitamin D supply related to the maintenance of calcium–phosphate homeostasis and the regulation of cell proliferation/differentiation. This study aimed to analyze the concentration of 25-hydroxycholecalciferol and its relationship with severe condition after burn injury. Methods: 126 patients were enrolled in the study. Patients were qualified due to thermal burns—over 10% of total body surface area. On the day of admission, the following parameters were assessed: 25-hydroxycholecalciferol concentration, total protein concentration, albumin concentration, aspartate transaminase activity, alanine transaminase activity, albumin concentration, creatinine concentration, c-reactive protein concentration, procalcitonin concentration, and interleukin-6 concentration. Results: Almost all patients (92%) in the study group had an improper level of vitamin D (<30 ng/mL), with the average of 11.6 ± 10.7 ng/mL; 17.5% of patients had levels of vitamin D below the limit of determination—under 3 ng/mL. The study showed that there are several factors which correlated with vitamin D concentration during the acute phase of burn injury, including: total protein (r = 0.42, p < 0.01), albumin, (r = 0.62, p < 0.01), percentage of body burns (r = 0.36, p < 0.05), aspartate aminotransferase (r = 0.21, p < 0.05), and c-reactive protein (r = 0.22, p < 0.05). We did not find any significant correlation between vitamin D concentration and body mass index. Conclusions: The burn injury has an enormous impact on the metabolism and the risk factors of the deficiency for the general population (BMI) have an effect on burned patients. Our study showed that concentration of 25-hydroxycholecalciferol is strongly correlated with serum albumin level, even more than total burn surface area and burn degrees as expected. We suspect that increased supplementation of vitamin D should be based on albumin level and last until albumin levels are balanced.
Transfersomes: A Promising Nanoencapsulation Technique for Transdermal Drug Delivery
Authors: Opatha Shakthi Apsara Thejani , Titapiwatanakun Varin , Chutoprapat Romchat
Published: September 2020

Transdermal delivery systems have gained much interest in recent years owing to their advantages compared to conventional oral and parenteral delivery systems. They are noninvasive and self-administered delivery systems that can improve patient compliance and provide a controlled release of the therapeutic agents. The greatest challenge of transdermal delivery systems is the barrier function of the skin’s outermost layer. Molecules with molecular weights greater than 500 Da and ionized compounds generally do not pass through the skin. Therefore, only a limited number of drugs are capable of being administered by this route. Encapsulating the drugs in transfersomes are one of the potential approaches to overcome this problem. They have a bilayered structure that facilitates the encapsulation of lipophilic and hydrophilic, as well as amphiphilic, drug with higher permeation efficiencies compared to conventional liposomes. Transfersomes are elastic in nature, which can deform and squeeze themselves as an intact vesicle through narrow pores that are significantly smaller than its size. This review aims to describe the concept of transfersomes, the mechanism of action, different methods of preparation and characterization and factors affecting the properties of transfersomes, along with their recent applications in the transdermal administration of drugs.
Role of OATP1B1 and OATP1B3 in Drug-Drug Interactions Mediated by Tyrosine Kinase Inhibitors
Authors: Garrison Dominique A. , Talebi Zahra , Eisenmann Eric D. , Sparreboom Alex , Baker Sharyn D.
Published: September 2020

Failure to recognize important features of a drug’s pharmacokinetic characteristics is a key cause of inappropriate dose and schedule selection, and can lead to reduced efficacy and increased rate of adverse drug reactions requiring medical intervention. As oral chemotherapeutic agents, tyrosine kinase inhibitors (TKIs) are particularly prone to cause drug-drug interactions as many drugs in this class are known or suspected to potently inhibit the hepatic uptake transporters OATP1B1 and OATP1B3. In this article, we provide a comprehensive overview of the published literature and publicly-available regulatory documents in this rapidly emerging field. Our findings indicate that, while many TKIs can potentially inhibit the function of OATP1B1 and/or OATP1B3 and cause clinically-relevant drug-drug interactions, there are many inconsistencies between regulatory documents and the published literature. Potential explanations for these discrepant observations are provided in order to assist prescribing clinicians in designing safe and effective polypharmacy regimens, and to provide researchers with insights into refining experimental strategies to further predict and define the translational significance of TKI-mediated drug-drug interactions.
Biocompatible Catanionic Vesicles from Arginine-Based Surfactants: A New Strategy to Tune the Antimicrobial Activity and Cytotoxicity of Vesicular Systems
Authors: Pinazo Aurora , Pons Ramon , Marqués Ana , Farfan Maribel , da Silva Anderson , Perez Lourdes
Published: September 2020

Their stability and low cost make catanionic vesicles suitable for application as drug delivery systems. In this work we prepared catanionic vesicles using biocompatible surfactants: two cationic arginine-based surfactants (the monocatenary Nα-lauroyl-arginine methyl ester—LAM and the gemini Nα,Nϖ-bis(Nα-lauroylarginine) α, ϖ-propylendiamide—C3(CA)2) and three anionic amphiphiles (the single chain sodium dodecanoate, sodium myristate, and the double chain 8-SH). The critical aggregation concentration, colloidal stability, size, and charge density of these systems were comprehensively studied for the first time. These catanionic vesicles, which form spontaneously after mixing two aqueous solutions of oppositely charged surfactants, exhibited a monodisperse population of medium-size aggregates and good stability. The antimicrobial and hemolytic activity of the vesicles can be modulated by changing the cationic/anionic surfactant ratio. Vesicles with a positive charge efficiently killed Gram-negative and Gram-positive bacteria as well as yeasts; the antibacterial activity declined with the decrease of the cationic charge density. The catanionic systems also effectively eradicated MRSA (Methicillin-resistant Staphylococcus Aureus) and Pseudomonas aeruginosa biofilms. Interestingly, the incorporation of cholesterol in the catanionic mixtures improved the stability of these colloidal systems and considerably reduced their cytotoxicity without affecting their antimicrobial activity. Additionally, these catanionic vesicles showed good DNA affinity. Their antimicrobial efficiency and low hemolytic activity render these catanionic vesicles very promising candidates for biomedical applications.
Berberine-Loaded Liposomes for the Treatment of Leishmania infantum-Infected BALB/c Mice
Authors: Calvo Alba , Moreno Esther , Larrea Esther , Sanmartín Carmen , Irache Juan Manuel , Espuelas Socorro
Published: September 2020

Berberine (BER)—an anti-inflammatory quaternary isoquinoline alkaloid extracted from plants—has been reported to have a variety of biologic properties, including antileishmanial activity. This work addresses the preparation of BER-loaded liposomes with the aim to prevent its rapid liver metabolism and improve the drug selective delivery to the infected organs in visceral leishmaniasis (VL). BER liposomes (LP-BER) displayed a mean size of 120 nm, negative Z-potential of −38 mV and loaded 6 nmol/μmol lipid. In vitro, the loading of BER in liposomes enhanced its selectivity index more than 7-fold by decreasing its cytotoxicity to macrophages. In mice, LP-BER enhanced drug accumulation in the liver and the spleen. Consequently, the liposomal delivery of the drug reduced parasite burden in the liver and spleen by three and one logarithms (99.2 and 93.5%), whereas the free drug only decreased the infection in the liver by 1-log. The organ drug concentrations—far from IC50 values— indicate that BER immunomodulatory activity or drug metabolites also contribute to the efficacy. Although LP-BER decreased 10-fold—an extremely rapid clearance of the free drug in mice—the value remains very high. Moreover, LP-BER reduced plasma triglycerides levels.
Recent Advances in the Development of In Situ Gelling Drug Delivery Systems for Non-Parenteral Administration Routes
Authors: Vigani Barbara , Rossi Silvia , Sandri Giuseppina , Bonferoni Maria Cristina , Caramella Carla M. , Ferrari Franca
Published: September 2020

In situ gelling drug delivery systems have gained enormous attention over the last decade. They are in a sol-state before administration, and they are capable of forming gels in response to different endogenous stimuli, such as temperature increase, pH change and the presence of ions. Such systems can be administered through different routes, to achieve local or systemic drug delivery and can also be successfully used as vehicles for drug-loaded nano- and microparticles. Natural, synthetic and/or semi-synthetic polymers with in situ gelling behavior can be used alone, or in combination, for the preparation of such systems; the association with mucoadhesive polymers is highly desirable in order to further prolong the residence time at the site of action/absorption. In situ gelling systems include also solid polymeric formulations, generally obtained by freeze-drying, which, after contact with biological fluids, undergo a fast hydration with the formation of a gel able to release the drug loaded in a controlled manner. This review provides an overview of the in situ gelling drug delivery systems developed in the last 10 years for non-parenteral administration routes, such as ocular, nasal, buccal, gastrointestinal, vaginal and intravesical ones, with a special focus on formulation composition, polymer gelation mechanism and in vitro release studies.
Opioid Use Disorders in People Living with HIV/AIDS: A Review of Implications for Patient Outcomes, Drug Interactions, and Neurocognitive Disorders
Authors: Cernasev Alina , Veve Michael P. , Cory Theodore J. , Summers Nathan A. , Miller Madison , Kodidela Sunitha , Kumar Santosh
Published: September 2020

The opioid epidemic has had a significant, negative impact in the United States, and people living with HIV/AIDS (PLWHA) represent a vulnerable sub-population that is at risk for negative sequela from prolonged opioid use or opioid use disorder (OUD). PLWHA are known to suffer from HIV-related pain and are commonly treated with opioids, leading to subsequent addictive disorders. PLWHA and OUD are at an increased risk for attrition in the HIV care continuum, including suboptimal HIV laboratory testing, delayed entry into HIV care, and initiation or adherence to antiretroviral therapy. Barriers to OUD treatment, such as medication-assisted therapy, are also apparent for PLWHA with OUD, particularly those living in rural areas. Additionally, PLWHA and OUD are at a high risk for serious drug–drug interactions through antiretroviral-opioid metabolic pathway-related inhibition/induction, or via the human ether-a-go-go-related gene potassium ion channel pathways. HIV-associated neurocognitive disorders can also be potentiated by the off-target inflammatory effects of opioid use. PLWHA and OUD might require more intensive, individualized protocols to sustain treatment for the underlying opioid addiction, as well as to provide proactive social support to aid in improving patient outcomes. Advancements in the understanding and management of PLWHA and OUD are needed to improve patient care. This review describes the effects of prescription and non-prescription opioid use in PLWHA.
Performance of Heat-Insulating Materials Doped with Basalt Fibres for Use in Mines
Authors: Jiang Yewei , Xin Song , Li Hongyu , Zhang Long , Hou Chuanbin , Zhang Zhaopeng , Guo Jianghui
Published: September 2020

To solve high-temperature-induced hazards in mines, heat-insulating materials were prepared by utilising basalt fibres and high-strength ceramsite combined with cementing materials. Through orthogonal tests and data analyses, the optimal combination of the heat-insulating materials doped with basalt fibres was determined as A1B1C1, that is, doping with 45% basalt fibres, a length of the basalt fibres of 6 mm, and doping with 20% ceramsite. The performance indices corresponding to the optimal comprehensive combination of the heat-insulating materials doped with basalt fibres included a density of 1200 kg/m3, thermal conductivity of 0.151 W/(mK), compressive strength of 9.7 MPa, flexural strength of 3.6 MPa, and a water-seepage depth of 25.4 mm. Numerical simulations verified that the materials presented favourable thermal insulation performance.
Polymer Composite Materials Fiber-Reinforced for the Reinforcement/Repair of Concrete Structures
Authors: Soupionis George , Georgiou Pantelitsa , Zoumpoulakis Loukas
Published: September 2020

The present paper deals with the use of polymeric matrix composite materials reinforced with carbon fiber as concrete shear reinforcement materials. Accordingly, cement specimens were manufactured and coated with various types of carbon fabrics and epoxy resin in liquid and solid form (paste). Additionally, composite materials of epoxy resin matrix reinforced with carbon fiber fabrics were manufactured. In all the specimens, the mechanical properties were estimated; the cement samples coated with composite materials of epoxy resin matrix reinforced with carbon fiber fabrics were tested for compressive strength, while the other specimens were tested for shear and bending strength. The specimens were subjected to artificial aging through heat treatment for 8, 12 and 16 days. During the process of artificial aging, the temperature in the chamber reached the range of 65–75 °C. These composite materials exhibited high mechanical properties combined with adaptability. Both an external deterioration of the materials as well as a reduction in mechanical properties during their artificial aging heat treatment were observed. This was shown in the specimens that were not subjected to artificial aging, with an applied compression strength of 74 MPa, and after the artificial aging, there was a decrease of ~7%, with the compression strength being reduced to 68 MPa.
Construction and Transition Metal Oxide Loading of Hierarchically Porous Carbon Aerogels
Authors: Wang Jintian , Ruan Xinyang , Qiu Jiahao , Liang Hao , Guo Xingzhong , Yang Hui
Published: September 2020

Hierarchically porous carbon aerogels (CAs) were prepared by organic condensation gelation method combined with atmospheric drying and pore-formation technology, followed by a carbonization process. With as-prepared CAs as substrate, the transition metal oxide nanoparticles loaded CAs composites (MnO2/Mn2O3@CA and Ni/NiO@CA) were achieved by means of liquid etching method combined with heat treatment, respectively. The catalyst, pore-forming agent and etching have important roles on the apparent density and pore structure of CAs. The hydrochloric acid (catalyst) significantly accelerates the gelation process and influences the size and distribution of macropores, whereas the addition of PEG2000 (pore-forming agent) and the etching of liquid solution leads to the formation of mesopore structure in CAs. Appropriate amounts of hydrochloric acid and PEG2000 allow the formation of hierarchically porous CAs with a BET surface area of 482.9 m2·g−1 and a macropore size of 11.3 μm. After etching and loading, the framework of CAs is etched to become a mesoporous structure, and the transition metal oxide nanoparticles can be uniformly loaded in CAs. These resultant composites have promising application in super capacitor, electrocatalysis, batteries and other fields.
Multivariate Six Sigma: A Case Study in Industry 4.0
Authors: Palací-López Daniel , Borràs-Ferrís Joan , da Silva de Oliveria Larissa Thaise , Ferrer Alberto
Published: September 2020

The complex data characteristics collected in Industry 4.0 cannot be efficiently handled by classical Six Sigma statistical toolkit based mainly in least squares techniques. This may refrain people from using Six Sigma in these contexts. The incorporation of latent variables-based multivariate statistical techniques such as principal component analysis and partial least squares into the Six Sigma statistical toolkit can help to overcome this problem yielding the Multivariate Six Sigma: a powerful process improvement methodology for Industry 4.0. A multivariate Six Sigma case study based on the batch production of one of the star products at a chemical plant is presented.
A Comparative Study between Bimetallic Iron@copper Nanoparticles with Iron and Copper Nanoparticles Synthesized Using a Bioflocculant: Their Applications and Biosafety
Authors: Dlamini Nkosinathi Goodman , Basson Albertus Kotze , Pullabhotla Viswanadha Srirama Rajasekhar
Published: September 2020

Nanotechnology addresses numerous environmental problems such as wastewater treatment. Ground water, surface water and wastewater that is contaminated by toxic organic, inorganic solutes and pathogenic microorganisms can now be treated through the application of nanotechnology. The study reports iron@copper (Fe@Cu) nanoparticles, iron nanoparticles (FeNPs) and copper nanoparticles (CuNPs) synthesized using a bioflocculant in a green approach technique. Characterization of the as-synthesized materials was achieved using analytical techniques such as Fourier transform-Infrared spectroscopy (FT-IR), Thermogravimetric analysis (TGA), Scanning Electron Microscope (SEM), Transmission Electron Microscopy (TEM), UV-Vis spectroscopy (UV-Vis) and X-ray diffraction (XRD). The presence of hydroxyl (–OH) and amine (–NH2) groups was shown by FT-IR spectroscopy studies and the as-synthesized material was shown to be thermostable. Elements such as oxygen, carbon, iron and copper were found to be abundant in Wt%. Absorption peaks were found between 200 and 390 nm wavelength and diffraction peaks at 2θ –29°, 33° and 35° for FeNPs, CuNPs and Fe@Cu, respectively. In their application, the effect of various parameters on the flocculation activity were evaluated. Both the CuNPs and (Fe@Cu) nanoparticles have shown the best flocculation activity at a concentration of 0.2 mg/mL with over 90% activity, while the dosage size with a concentration of 0.4 mg/mL was optimal for FeNPs. The FeNPs were found to be cation dependent, while CuNPs and Fe@Cu nanoparticles flocculate in the absence of a cation and flocculate both in acidic and alkaline pH. All the synthesized nanoparticles are thermostable and maintain flocculation activity above 80% at 100 °C. Both the Fe@Cu and CuNPs were found to be effective in removing dyes with the removal efficiency above 89% and were found to be effective in removal of chemical oxygen demand (COD) and biochemical oxygen demand (BOD) in Mzingazi river water and coal mine wastewater with over 80% removal efficiency. Moreover, the synthesized nanoparticles showed some remarkable antimicrobial properties when evaluated against Gram-positive and Gram-negative bacteria. The as-synthesized material was found to be safe to use at low concentration when verified against human embryonic cells (HEK293) and breast cancer cells (MCF7) and biodegradable.