bluuebunny/crossref_metadata_2025 huggingface dataset:
An archaeal compound as a driver of Parkinson’s disease pathogenesis
Authors: Wilmes Paul , Trezzi Jean-Pierre , Aho Velma , Jäger Christian , Schade Sebastian , Janzen Annette , Hickl Oskar , Kunath Benoit , Thomas Mélanie , Schmit Kristopher , Garcia Pierre , Sciortino Alessia , Martin-Gallausiaux Camille , Halder Rashi , Huarte Oihane Uriarte , Heurtaux Tony , Heins-Marroquin Ursula , Gomez-Giro Gemma , Weidenbach Katrin , Delacour Léa , Laczny Cédric , Novikova Polina , Ramiro-Garcia Javier , Singh Randolph , Andújar Begoña Talavera , Lebrun Laura , Daujeumont Annegrait , Habier Janine , Dong Xiangyi , Gavotto Floriane , Heintz-Buschart Anna , Schneider Jochen , Jehmlich Nico , von Bergen Martin , Schymanski Emma , Schmitz Ruth , Schwamborn Jens , Glaab Enrico , Linster Carole , Kitami Toshimori , Buttini Manuel , May Patrick , Trenkwalder Claudia , Oertel Wolfgang , Mollenhauer Brit
Published: July 2022

Patients with Parkinson’s disease (PD) exhibit differences in their gut microbiomes compared to healthy individuals. Although differences have most commonly been described in the abundances of bacterial taxa, changes to viral and archaeal populations have also been observed. Mechanistic links between gut microbes and PD pathogenesis remain elusive but could involve molecules that promote α-synuclein aggregation. Here, we show that 2-hydroxypyridine (2-HP) represents a key molecule for the pathogenesis of PD. We observe significantly elevated 2-HP levels in faecal samples from patients with PD or its prodrome, idiopathic REM sleep behaviour disorder (iRBD), compared to healthy controls. 2-HP is correlated with the archaeal species Methanobrevibacter smithii and with genes involved in methane metabolism, and it is detectable in isolate cultures of M. smithii. We demonstrate that 2-HP is selectively toxic to transgenic α-synuclein overexpressing yeast and increases α-synuclein aggregation in a yeast model as well as in human induced pluripotent stem cell derived enteric neurons. It also exacerbates PD-related motor symptoms, α-synuclein aggregation, and striatal degeneration when injected intrastriatally in transgenic mice overexpressing human α-synuclein. Our results highlight the effect of an archaeal molecule in relation to the gut-brain axis, which is critical for the diagnosis, prognosis, and treatment of PD.
Characterization of a multifunctional biocontrol agent, Streptomyces Misakimycin, against Newcastle Disease Virus
Authors: Abdelaziz Rewan , Barakat Ahmed B. , EL-Didamony Gamal , Tartor Yasmine H. , Gado Marwa M. , El-Samadony Hanaa A.
Published: July 2022

Background
The Newcastle Disease Virus (NDV) is present throughout the world, and outbreaks in Egypt caused serious economic losses in the poultry industry. Actinobacteria are a phylum of bacteria known for their potential in producing structurally diversified natural products which promising natural compounds used to combat viruses are presented and evaluated. Streptomyces Misakimycin isolated from Egyptian soil, and evaluated for their efficacy in controlling NDV. On the basis of the biochemical characteristics S. Misakimycin metabolites, identified by infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy.
Results
In this investigation, NDV was found to have been isolated in February from a chicken farm in the Dakahlia Governorate of Egypt by the Animal Health Research Institute in Dokki, Giza. Diethylpthalate (DEP), a secondary metabolite of S. misakimycin, completely inhibited the hemagglutination (HI) activity of the NDV strain (MN635617) at log107 infectivity titers (EID50/mL). With 0.5 percent and 0.75 percent of chicken RBCs, the HA of the NDV strain was 2 log2 and 5 log2, respectively. In the treated group that received DEP and NDV inoculations, chorioallantoic-membranes (CAM) structures were preserved along with dilated capillary networks. Histological changes in SPF-ECE liver were examined after inoculation in ova to further characterize the DEP effect. Diethylpthalate and NDV mixture inoculated group showed preserved cytoarchitecture of hepatocytes with the presence of perivascular foci of lymphocytes. The group that was inoculated with telomycin alone showed normal histology of hepatic acini, central veins, and portal triads.
Conclusion
A potentially bioactive substance called diethylpthalate has the potential to be a biological weapon against a fatal chicken NDV that quickly increase cost losses for farmers.
The Impact of Atrophic Gastritis and Intestinal Metaplasia on the Occurrence of Metachronous Gastric Cancer After Endoscopic Resection: A Systematic Review and Meta-analysis
Authors: Choe Younghee , Park Jae Myung , Kim Joon Sung , Cho Yu Kyung , Kim Byung-Wook , Choi Myung-Gyu
Published: July 2022

Background: Metachronous gastric cancer (MGC) can occur after endoscopic resection for gastric cancer. This systematic review and meta-analysis aimed to evaluate the risk of MGC caused by severe atrophic gastritis (AG) and intestinal metaplasia (IM).Methods: We searched the medical literature through May 2022 and identified patients who occurred MGC after endoscopic resection for gastric cancer. We retrieved studies evaluating the degree of AG and the presence or absence of IM and compared the incidence of MGC, respectively.Results: From nine cohort studies, 2,755 patients by 2018. Incidence of MGC was significantly higher in severe AG (RR, 1.64; 95% CI, 1.29–2.07; I2=45%) and IM (RR, 7.08; 95% CI, 3.63–13.80; I2=0%) than those without. The absolute risk difference of MGC in severe AG and IM was 7.5% and 9.2%, respectively. The difference in incidence rate per 1000 person-years was 17.5 and 22.5 person-years, respectively. However, the H. pylori infection did not affect the MGC occurrence (RR 1.08, 95% CI 0.85–1.39, I2=0%).Conclusions: Severe AG or IM had a 1.6-fold and 7.0-fold higher risk of MGC occurrence after endoscopic resection of gastric cancer. Patients with severe AG or IM need more stringent follow-up to monitor MGC occurrences.
The H3 K27-altered, Extent of Resection and Survival in Pediatric High-grade Spinal Cord Gliomas
Authors: Yu Qi-Shuai , Zhang Fan , Fan Tao , Yu Xin-Guang
Published: July 2022

Purpose
Pediatric high-grade spinal cord gliomas (pHGSG) are classified into two prognostically meaningful subtypes. The purpose of our study is to describe clinical characteristics and management of two pHGSG subtypes, especially focus on the effect of surgical resection in these two subtypes.
Methods
We described the clinical characteristics and management and analyzed the prognostic factors of 19 pHGSGs. Based on H3 K27-altered and isocitrate dehydrogenase (IDH) mutation status, pHGSGs were classified into H3 K27-altered and H3/IDH-wildtype pHGSG for assessing effect of surgical resection.
Results
Nineteen patients (11 males and 8 females) with a mean age of 12.7 ± 4.7 years were included in the analysis. The median survival time of all patients was 14 months. Thirteen pHGSG patients had H3 K27-altered tumors, and 6 had H3/IDH-wildtype tumors. All patients received therapeutic surgical resection, 10 (55.6%) patients underwent gross total resection (GTR) and 8 (33.3%) patients underwent subtotal resection (STR). pHGSG patients with H3 K27-altered underwent GTR (HR 0.311, 95% CI 0.075–1.289, p = 0.091) had tended to increase overall survival. For patients of H3/IDH-wildtype pHGSG, there was no survival advantage associated with GTR (p = 0.157).
Conclusions
The extent of surgical resection has the different effects on survival in H3 K27-altered and H3/IDH-wildtype pHGSGs. The patients with H3 K27-altered pHGSG have tended to benefit from a greater extent of surgical resection. Nevertheless the benefit was not evident in the patients with H3/IDH-wildtype pHGSG.
A novel method for image captioning using multimodal feature fusion employing mask recurrent neural networks and long short term memory
Authors: Thangavel Kumaravel , Palanisamy Natesan , Muthusamy Suresh , Panchal Hitesh
Published: July 2022

The image captioning is a technique that allows us to use computers to interpret the information of photographs and make written text. The use of deep learning to interpret image information and create descriptive text has become a widely researched issue since its establishment. Nevertheless, these strategies do not identify all samples that depict conceptual ideas. By reality, the vast majority of them seem to be irrelevant to the matching tasks. The degree of similarity is determined only by a few relevant semantics occurrences. These duplicate instances can also be thought of as noise, as they obstruct the matching process of a few meaningful instances and add to the model's computational effort. Furthermore, present approaches frequently require deliberate use of additional target recognition algorithms or costly human labelling in the extracting information is required.For image captioning, this research presents a multi-modal feature fusion based deep learning model. The coding layer uses Mask Recurrent Neural Networks (Faster R-CNN), the long short-term memory (LSTM)-attend has been used to decoding, and the descriptive text is constructed.In deep learning, the model parameters are optimized through method gradients optimization. In the decoding layer, dense attention mechanisms can assist in minimizing non-salient data interruption and preferentially input the appropriate comprised for the decryption stage. The model's capacity to comprehend images and generate text is validated by the experimental data in the domain of generic images. This paper is implemented using Python frameworks and also evaluated using the performance metrics such as PSNR, RMSE, SSIM, Accuracy, Recall, F1-score and Precision.
The Interaction of Specific Frequency Bands in the Geomagnetic Field Diurnal Spectrum, With Specific Frequency Bands in the Human Heart Rate Variability Diurnal Spectrum.
Authors: Davies Elizabeth Anne
Published: July 2022

BACKGROUND.
For over 50 years, experiments have been demonstrating an interaction between the dynamics of the human heart and the dynamics of the earth’s magnetic field. In these experiments, specific frequency components in the variability of the geomagnetic field (GMF), involving micropulsations, are shown to significantly interact with specific frequency components in human heart rate variability (HRV). A significant difference was found in the HRV ratio of VLF/HF, on days of low geomagnetic variability (L gmf) compared with days of high geomagnetic field variability (H gmf) (P = 0.0001, n = 24, effect size 1.014).
METHODS.
Experiments were conducted prospectively, over a period of 3 years, on one 70 year old, healthy female subject, using a 24 hour holter electrocardiogram (ECG) machine, and the results were analysed for the HRV frequency spectral components. The local geomagnetic field was simultaneously monitored over 24 hour periods, using a 3-axis fluxgate magnetometer, and the frequency spectra were analysed for the various spectral power components. Statistical analysis was performed using XLSTAT software.
RESULTS.
It was shown that there was a correspondence in the power spectra of the high (HF) and very low (VLF) frequency components in HRV and GMF variability. It was shown that there was a significant difference in the ratio of the VLF and HF frequency band power of the HRV between the ECGs recorded during days of lower geomagnetic variability, and days of higher geomagnetic variability.
CONCLUSION.
These findings support the hypothesis of a homeostatic response of the human heart to the earth’s magnetic field. This may have implications for prognosis and management of heart disease, during times of significant environmental change.
Safety and efficacy of host-associated Latilactobacillus curvatus strains for potential probiotic applications in farmed Atlantic salmon (Salmo salar)
Authors: Cathers Hannah S. , Mane Shrinivasrao P. , Tawari Nilesh R. , Balakuntla Jayanth , Plata Germán , Krishnamurthy Madan , MacDonald Alicia , Wolter Marilyn , Baxter Niel , Briones Julian , Nagireddy Akshitha , Millman Gregory , Martin Roberto E. , Kumar Arvind , Gangaiah Dharanesh
Published: July 2022

Salmon aquaculture is the fastest growing animal protein production system in the world; however, intensive farming leads to poor weight gain, stress, and disease outbreaks. Probiotics offer the potential to enhance growth performance and feed efficiency in Atlantic salmon, as well as immunostimulate fish against common pathogens, benefitting farmers and consumers with more efficient production. Here, we isolated and identified 900 native microbial isolates including 18 Lactobacilli from farmed salmon intestines. Based on whole-genome sequencing and phylogenetic analysis, the Lactobacillus candidates belonged to Latilactobacillus curvatus (L. curvatus) species and formed two distinct phylogenetic groups. Using bioinformatics and in vitro analyses, we selected two candidates L. curvatus ATCC PTA-127116 and L. curvatus ATCC PTA-127117, which showed desirable safety and probiotic properties. The two L. curvatus candidates were evaluated for safety and efficacy in Atlantic salmon alongside spore-forming Bacilli isolated from salmon, poultry, and swine. All the tested candidates were safe to salmon with no adverse effects. While we did not see efficacy in any Bacillus supplemented groups, compared to untreated group, the group administered with the two L. curvatus strains consortium in feed for seven weeks showed significant improvement in final body weight by 4.2%. Similarly, the two L. curvatus candidates were also evaluated for safety and efficacy in Atlantic salmon in saltwater; the group administered with the two L. curvatus strains consortium in feed for 11 weeks showed significant improvement in final body weight by 4.7%. Comprehensive metabolomics analyses in the presence of different prebiotics and/or additives identified galactooligosaccharide as a potential prebiotic to enhance the efficacy of two L. curvatus candidates. All together, these data provide in silico and in vivo evidence of safety and efficacy of two novel endogenous L. curvatus candidates for potential economical and sustainable probiotic applications in salmon and likely other aquatic species.
Distribution of relief items with specific demand and inventory constraints using a multi-product optimization model
Authors: Singh Jitendra , Jaggi Chandra K.
Published: July 2022

The humanitarian relief chain is one of the most critical components of a disaster relief operation. The present work develops a nonlinear programming approach for disaster relief logistics under risk with uncertainty. In this approach, demands and supplies and the cost of inventory and transportation are considered depending on the severity of the disaster. Furthermore, the model considers the demand for the affected locations where the demand fluctuates due to the new entrants and individuals leaving relief camps. The discussed multi-product inventory relief chain model attempts to minimize the sum of the relief chain's transportation and total costs while considering inventory and disposal of damaged units. The transportation cost is affected by the severity of the disaster and a risk factor associated with affected locations. Assuming these costs, mixed-integer nonlinear programming is constructed and demonstrated with a numerical example. A numerical example with sensitivity analysis with multiple cost parameters is presented to improve relief supply chain planning in disaster scenarios. The results suggest that the proposed model can help decision-making about facility location and relief supplies distribution during disaster relief efforts.
The behaviour of the Gauss-Radau upper bound of the error norm in CG
Authors: Meurant Gérard , Tichý Petr
Published: July 2022

Consider the problem of solving systems of linear algebraic equations Ax=b with a real symmetric positive definite matrix A using the conjugate gradient (CG) method. To stop the algorithm at the appropriate moment, it is important to monitor the quality of the approximate solution. One of the most relevant quantities for measuring the quality of the approximate solution is the A-norm of the error. This quantity cannot be easily evaluated, however, it can be estimated. In this paper we discuss and analyze the behaviour of the Gauss-Radau upper bound on the A-norm of the error, based on viewing CG as a procedure for approximating a certain Riemann-Stieltjes integral. This upper bound depends on a prescribed underestimate μ to the smallest eigenvalue of A. We concentrate on explaining a phenomenon observed during computations showing that, in later CG iterations, the upper bound loses its accuracy, and it is almost independent of μ. We construct a model problem that is used to demonstrate and study the behaviour of the upper bound in dependence of μ, and developed formulas that are helpful in understanding this behavior. We show that the above mentioned phenomenon is closely related to the convergence of the smallest Ritz value to the smallest eigenvalue of A. It occurs when the smallest Ritz value is a better approximation to the smallest eigenvalue than the prescribed underestimate μ. We also suggest an adaptive strategy for improving the accuracy of the Gauss-Radau upper bound such that the resulting estimate approximates the quantity of interest with a prescribed relative accuracy.
RNA polymerase II and PARP1 shape enhancer-promoter contacts
Authors: Danko Charles , Barshad Gilad , Lewis James , Chivu Alexandra , Abuhashem Abderhman , Krietenstein Nils , Rice Edward , Rando Oliver J. , Hadjantonakis Anna-Katerina
Published: July 2022

How enhancers control target gene expression over long genomic distances remains an important unsolved problem. Here we studied enhancer-promoter contact architecture and communication by integrating data from nucleosome-resolution genomic contact maps, nascent transcription, and perturbations to transcription-associated proteins and thousands of candidate enhancers. Contact frequency between functionally validated enhancer-promoter pairs was most enriched near the +1 and +2 nucleosomes at enhancers and target promoters, indicating that functional enhancer-promoter pairs spend time in close physical proximity. Blocking RNA polymerase II (Pol II) caused major disruptions to enhancer-promoter contacts. Paused Pol II occupancy and the enzymatic activity of poly (ADP-ribose) polymerase 1 (PARP1) stabilized enhancer-promoter contacts. Based on our findings, we propose an updated model that couples transcriptional dynamics and enhancer-promoter communication.
Comparing Avian Species Richness Estimates from Structured and Semi-Structured Citizen- Science Data
Authors: Shen Fang-Yu , Ding Tzung-Su , Tsai Jo-Szu
Published: July 2022

Citizen science, including structured and semi-structured forms, has become a powerful tool to collect biodiversity data. However, semi-structured citizen science data have been criticized for higher variability in quality, including less information to adjust for imperfect detection. Species richness estimators may quantify bias in estimates. Here, we test the effectiveness of Chao1 estimator in eBird (semi-structured) by comparing it to averaged species richness in Breeding Bird Survey Taiwan, BBS (structured) and quantifying bias. We then fit a power function to compare bias while controlling for differences in count duration. The Chao1 estimator increased the species richness estimates from eBird data from 56% to 75% of the BBS. When singleton species predominated in eBird data, Chao1 overestimated richness by 7.45 times. Effects of short duration samples and variability in detectability skills of observers can lead to the biased estimates. Using the Chao1 estimator improved estimates of species richness from semi-structured data, but the strong effect of singleton species on bias, especially in short duration counts, should be evaluated in advance to reduce the uncertainty of estimation processes.
2L polyethylene glycol combined with castor oil versus 4L polyethylene glycol for bowel preparation before colonoscopy among inpatients
Authors: Xiong Zhe , Fang Ying , Feng Fangfang , Cheng Yiming , Zhang Huahui , Huo Chunyan , Huang Jin
Published: July 2022

Objectives: The study aimed to compare the cleansing effect, compliance and adverse of polyethylene glycol 2L with castor oil and polyethylene glycol 4L on bowel preparation prior to colonoscopy in inpatients.Methods: Our study retrospectively analyzed the medical records and colonoscopy reports of inpatients (n=673) who underwent colonoscopy at the Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, and the inpatients were divided into two groups according to different bowel preparation protocols. BBPS is used to assess the degree of bowel cleanliness before colonoscopy. We statistically analyzed and compared the degree of bowel cleansing between 2L-PEG-CaO and 4L-PEG group, as well as the compliance and adverse effects of the medication in both groups.Results: Among 673 patients undergoing colonoscopy, 330 were prescribed 4L-PEG and 343 2L-PEG-CAO. Both groups of inpatients were matched for baseline characteristics. In the 4L-PEG and 2L-PEG-CaO groups, adequate bowel preparation rate was 72.4% and 77.3% (P=0.148), BBPS scores was 6.79±1.51 and 7.03±1.71 (P=0.056), and adverse event rate was 68.2% and 30.0% (P<0.001), respectively.Conclusion: The 2L-PEG combined with castor oil is an effective and safe cleansing regimen for bowel preparation and is relatively inexpensive and suitable for wider use.
The rural-urban divide of the relationship between polygynous marriage structures, multiple marriage unions, and self-rated happiness among currently married Ghanaian women
Authors: Ansah Kenneth Owusu , Dziwornu Emmanuel , Frimpong-Manso Kwabena , Dey Nutifafa Eugene Yaw , Duodu Precious Adade , Agbadi Pascal
Published: July 2022

Polygyny and multiple marital unions are generally associated with overall happiness deficits, yet the rural-urban differentials of these associations are not thoroughly interrogated. This study uses a nationally representative dataset of 7785 married and in-union Ghanaian women aged 15–49 years to investigate the rural-urban differences in the relationship between polygyny, multiple marital unions, and happiness. Findings show that rural married and in-union women in polygynous relationships [APOR = 0.760, 95% CI:0.60, 0.96] and were married more than once [APOR = 0.79, 95% CI:0.66, 0.94] were less likely to be happy. Age, education, household wealth, and region of residence were significantly associated with happiness only among rural married women. Both rural and urban married and in-union women in the Upper West and Upper East Regions were more likely to be happy than married women in the Greater Accra Region. Policies should enable rural women in polygyny and those in their second or more marital unions with happiness-enabling resources.
Modularity in design and production relationships: a field study in two automakers
Authors: Kubota Flavio Issao , Cauchick-Miguel Paulo Augusto , Hsuan Juliana , Lacerda Daniel Pacheco
Published: July 2022

Modularity-in-design (MID) and modularity-in-production (MIP) relationship has been gaining the attention of scholars and practitioners along the years. However, the literature does not specify how this connection occurs and its implications. This paper analyzes the relationship between MID and MIP within the automotive scenario and investigates its main technical and organizational implications. Through an abductive, matched-pair case study carried out in two large automakers, the study gathered data from interviews, on-site observations, and field notes from all interactions within the plants. The paper indicates that (i) both automakers prioritize modular design before modular production, characterizing a product-related approach, and (ii) the investigated automotive companies have different approaches when developing MID. One automaker focuses on product functionalities to develop the modules; the other company dedicates efforts towards a MID-MIP relationship through the industrial condominium concept. Results indicate significant propositions to be tested regarding MID-MIP connection, with original insights from two make-to-order (MTO) automotive companies.
Signal processing with MATLAB and Python for real-life structural health monitoring: A comparative implementation for post-processing
Authors: Patel Chandrabhan , Garg Naman , Panigrahi SK , Kumar Vikesh
Published: July 2022

Signal and data processing are necessary for analyzing the experimental data. Techniques of signal processing are well defined and handy for real-life application but applying those techniques to experimental data is challenging for the researcher. In structural health monitoring, post-processing helps remove noise and residuals from the data recorded through sensors. Various software and programming can help achieve the best signal processing algorithms. Each package has its solver to solve the signal processing algorithms. Due to the countless options available for signal processing, it is foremost to choose the best and most reliable package to solve the problem. Nowadays, MATLAB and Python are two packages that are reliable, popular, and accurate for multiple applications. This article compares MATLAB and Python for signal processing for structural health monitoring applications. File handling, simulating the signals, filter realization, filter response, and adding and removing noise from the signal are discussed and implemented for structural health monitoring. The real-life demonstration has been done using a simply supported beam, instrumented with Fiber Bragg Grating strain sensors. A comparative study of signal processing is done keeping structural health monitoring as the focus area.
Costunolide Protects Myocardial from Ischemia Reperfusion Injury through Nrf2 Activation
Authors: Li Weixin , Luo Yue , Huang Zhuqi , Shen Siyuan , Dai Chengyi , Shen Sirui , Liang Guang , Luo Wu
Published: July 2022

Purpose
Costunolide (Cos) is a naturally occurring sesquiterpene lactone that exhibits anti-oxidative properties. In this study, we demonstrated the protective mechanism of Cos against ischemia/reperfusion (I/R)-induced heart injury.
Methods
C57BL/6 mice were pretreated with Cos (10 mg/kg/day) or 1% CMC-Na for 1 week. The left anterior descending coronary artery (LAD) was ligated for 30 minutes for ischemia, and followed by ligation release for 4 hours for reperfusion. H9c2 cells challenged with tert-butyl hydroperoxide (TBHP) were used for in vitro studies.
Results
Pretreatment of Cos significantly reduced myocardial infarct size, serum CK-MB and LDH level in I/R injured heart. Cos administration significantly attenuated the amount of reactive oxygen species (ROS) and ameliorated the apoptosis both in in vitro and in vivo studies. Further investigation revealed that Cos significantly increased the expression of heme oxygenase 1 (HO-1) and NAD(P)H [quinone] dehydrogenase 1 (NQO-1). Meanwhile Cos increased B-cell lymphoma-2(Bcl-2) and decreased the BCL2-Associated X Protein (Bax) protein level. Silence of nuclear factor erythroid 2-related factor (Nrf2) significantly reverses the protective effect of Cos in TBHP-challenged H9C2 cells.
Conclusion
Our data clearly showed that Cos reduced TBHP challenged H9c2 cells and attenuated myocardial I/R injury through Nrf2 activation. These results indicate that Cos might be benefit in the therapy of myocardial I/R injury.
Clinical significance and potential application of cuproptosis-related genes in gastric cancer
Authors: Yan Jianing , Lin Yifan , Ye Guoliang , Shao Yongfu
Published: July 2022

Worldwide, gastric cancer (GC) is a common lethal solid malignancy with a poor prognosis. Cuproptosis is a novel kind of cell death mediated by protein lipoylation and the relationship between cuprotosis and GC prognosis. We estimated the expression level and prognostic values of pyroptosis-related genes (CRGs) in GC samples and related mechanisms using public databases and bioinformatics. Our results revealed that FDX1, LIAS, and MTF1 were differentially expressed in GC samples and exhibited important prognostic significance in the TCGA cohort. We constructed a nomogram model for overall survival and disease-specific survival prediction and validated it via calibration plots. Mechanistically, immune cell infiltration prominently affected the survival time of GC patients. Moreover, protein‒protein interaction network, KEGG pathway and gene ontology enrichment analyses demonstrated that FDX1, LIAS, MTF1 and related proteins played key roles in the tricarboxylic acid cycle and cuprotosis. GEO database validation showed that the expression levels of FDX1, LIAS, and MTF1 were consistent with those in the TCGA cohort. In conclusion, FDX1, LIAS, and MTF1 could serve as potential prognostic biomarkers for GC patients and provide novel targets for immunotarget therapy.
Progressive resolution optimizer (PRO) predominates over photon optimizer (PO) in sparing of spinal cord for spine SABR VMAT plans
Authors: Son Sangjun , Park So-Yeon
Published: July 2022

Background: we assessed the performance of the optimization algorithms by comparing volumetric modulated arc therapy generated by a progressive resolution optimized (VMATPRO) and photon optimizer (VMATPO) in terms of plan quality, MU reduction, sparing of the spinal cord (or cauda equina), and plan complexity.Methods: Fifty-seven patients who received spine stereotactic ablative radiotherapy (SABR) with tumors located in the cervical, thoracic, and lumbar spine were retrospectively selected. For each patient, VMATPRO and VMATPO with two full arcs were generated with using the PRO and PO algorithms. For dosimetric evaluation, the dose-volumetric (DV) parameters of the planning target volume (PTV), organs at risk (OARs), the corresponding planning organs at risk (PRV), and 1.5-cm ring structure surrounding the PTV (Ring1.5 cm) were calculate for all VMAT plans. The total number of monitor units (MUs) and the modulation complexity score for the VMAT (MCSv) were compared. To investigate the correlations of OAR sparing to plan complexity, Pearson’s and Spearman’s correlation tests were conducted between the two algorithms (PO – PRO, denoted as Δ) in the DV parameters for normal tissues, total MUs, and MCSv.Results: For the PTVs, Target conformity and dose homogeneity in the PTVs of VMATPRO were better than those of VMATPO with statistical significance. For the spinal cords (or cauda equine) and corresponding PRVs, all of the DV parameters for VMATPRO were markedly lower than those for VMATPO, with statistical significance (all p < 0.0001). Among them, the difference in the maximum dose to the spinal cord between VMATPRO and VMATPO was remarkable (9.04 Gy vs 11.08 Gy with p < 0.0001). For Ring1.5 cm, no significant difference in V115% for VMATPRO and VMATPO was observed.Conclusions: The use of VMATPRO resulted in coverage and uniformity of dose to the PTV, as well as OARs sparing, compared with that of VMATPO for cervical, thoracic, and lumbar spine SABR. Better dosimetric plan quality generated by the PRO algorithm was observed to result in higher total MUs and plan complexity. Therefore, careful evaluation of its deliverability should be performed with caution during the routine use of the PRO algorithm.
An edge detection algorithm for neutrosophic set based on adaptive threshold
Authors: Hu Zhaohui , Yang Liping , Du Zhanpeng , Na Yiming , Su Yulong
Published: July 2022

In image processing, edge detection is extremely important especially in feature detection. An edge detection algorithm for neutrosophic set based on adaptive threshold is proposed to effectively filter out the large amount of noise in the image and improve the detection speed and accuracy. The algorithm uses side window filtering algorithm to filter out noise based on neutrosophic set; Furthermore, it modifies the subset segmentation algorithm to split the image into three subsets of T, F, I; Then, an adaptive threshold extraction algorithm is proposed to segment the subset information; Finally, the segmentation information is merged to obtain edge features. In order to verify the effectiveness of the algorithm, an experiment is compared with the neutrosophic set edge detection algorithm based on maximum entropy (NMNE) under different noise conditions. An experimental comparison between Canny and Sober is also carried out additionally. Experimental results show that the algorithm has good anti-noise performance and improves the detection speed and accuracy of the neutrosophic set edge detection algorithm based on maximum entropy (NMNE). The algorithm proposed in this paper can provide an effective reference for mid- and high-level vision tasks.
A copper foam based surface enhanced Raman scattering substrate for glucose detection
Authors: Peng Wang , Xu Zhihan , Jia Xiangting , Liao Qingxi
Published: July 2022

Raman spectroscopy as a powerful characterization method for analyzing and testing the molecular structure of substances, which can quickly achieve non-destructive, qualitative and quantitative detection and analysis. Herein, a facile and low-cost method for preparation of highly sensitivity SERS substrates was implemented through the displacement reaction of copper foam immersed in AgNO3 ethanol solution. Due to the 3D structure of copper film and homogenous displacement, the Ag-Cu substrate showed high performance SERS enhancement (1.25 × 107), and the lowest detection concentration for R6G reached 10− 10 Mol/L. For glucose detection, mixed decanethiol (DT) / mercaptohexanol (MH) interlayer was used to enable glucose attach to the substrate surface, and the limit of detection reached to 1uM/L. The copper foam based SERS substrate makes the Ag-Cu SERS substrate promising for biological applications such diabetes testing.
Common genes regulating multiple abiotic stress response in rice: An analysis using supervised and unsupervised machine learning models
Authors: Sarkar Suman , Chidambaranathan Parameswaran , Jeughale Kishor , Sahoo Bandita , Sahoo Raj Kishore , Ray Hirakjyoti , Mohanty Lopamudra , Balasubramaniasai Cayalvizhi , Selvaraj Sabarinathan , Annamalai Anandan , Rathinam Prabhukarthikeyan Seenichamy , Behera Biswaranjan , Kumar Awadhesh , Katara Jawahar Lal , N Devanna Basanvantraya , Behera Lambodar , Samantaray Sanghamitra , Patra Bhaskar Chandra
Published: July 2022

Rice response to abiotic stresses is generally understood through comparison of gene expression and gene mapping between contrasting tolerant and susceptible genotypes. Machine learning uses large datasets and trains the data through building efficient models for pattern identification and feature importance (gene) prediction. This work explores the potential of using machine learning tools in identifying gene expression models common to different abiotic stress response in rice. For this, 146 rice microarray gene expression samples comprised of drought (57), salinity (35), cold (33) and heat (20) were categorized into two classes namely control, stress and analyzed using supervised (Random forest) and unsupervised (Cluster) machine learning algorithms. The best random forest trained model showed an accuracy of ~ 79% in the classification analysis with an ROC curve area of 0.7963. Besides, genes involved in ABA pathway, flowering, and secondary metabolites (Two PP2Cs, three expressed proteins, Flowering Promoter Factor-like, Terpene Synthase, Gamete EXpressed) were identified as common set of genes for multiple abiotic stress response in rice. Moreover, validation of these eight genes through q-PCR under vegetative stage drought stress identified intrinsic drought tolerance mechanism and drought responsiveness in drought tolerant and rainfed upland, aerobic, and irrigated rice varieties.
Establishment and validation of a nomogram model for predicting distant metastasis in medullary thyroid carcinoma: an analysis of the SEER database based on the AJCC 8th TNM staging system
Authors: Chen Zhufeng , Mao Yaqian , You Tingting , Chen Gang
Published: July 2022

Purpose: Medullary thyroid carcinoma (MTC) patients with distant metastasis frequently present a relatively poor survival prognosis. Our main purpose was developing anomogram model to predict distant metastasis in MTC patients.Methods: This was a retrospective study based on the Surveillance, Epidemiology, and End Results (SEER) database. Data of 852 MTC patientsdiagnosed from 2004 to 2015who undergone total thyroidectomy and neck lymph nodes dissection was included in our study. The log‐rank test was used to compare the differences of Kaplan-Meier curves of cancer-specific survival (CSS) in different groups. Independent risk factors were screened byunivariate and multivariate logistic regression analysissuccessively, which were used to develop a nomogram model predicting for distant metastasis risk.Results: CSS differed by different M stage, lymph node ratio (LNR) and age groups. Five clinical parametersincluding age>55 years, N1 stage, tumor size>40 mm, gross extra-thyroidal extension (ETE) and LNR>0.3were significantfor distant metastasis in MTC patients, which wereselectedto develop a nomogram. This model had satisfied discrimination with the AUC and C-index of 0.874, and C-index was confirmed to be 0.861 through bootstrapping validation. A decision curve analysis was subsequently made to evaluate the feasibility of this nomogram for predicting distant metastasis.Conclusions: Age, N stage, tumor size, gross ETE and LNR were extracted to develop a nomogram model for predicting the risk of distant metastasis in MTC. The model is of great significance for clinicians to timely identify patients with high risk of distant metastasis and make further clinical decisions.
Secondary metabolites with antibacterial activity produced by Quambalaria cyanescens isolated from unripened grapes
Authors: Mateo José Juan
Published: July 2022

Quambalaria cyanescens was identified on the surface of unripened grapes from the D. O. Manchuela (Spain); its behavour against some bacteria has been analyzed. Active compounds of each selected fungal strain were isolated, and their antibacterial activity assayed against various bacteria; the presence of inhibition zones and, therefore, the presence of secondary metabolites that inhibit bacterial growth were determined. This capacity was different according to the culture medium in which fungal strains were cultivated, and diverse inhibition zones were observed among bacteria used. Q. cyanescens produces metabolites with antibacterial activity so that microbiota on grapes surface represents a new ecological niche to be explored in the task of finding new antibiotics.
Comparison of the Effectiveness of Emotion Regulation Method based on Gross Model and Benson Muscle RelaxationTechnique on Resilience and Anger in Patients with Coronary Artery Disease
Authors: Ramazani Rahmat , mahale fatemeh mohammadi shir , Ranjbaripour Tahreh , Ahamdi Vahid , Peymani Javid
Published: July 2022

Background
Coronary heart disease which imposes a heavy burden on the health care system worldwide. Psychological factors are effective in the occurrence and persistence of this disease. The aim of this study was to compare the effectiveness of emotion regulation method based on Gross model and muscle relaxation technique on resilience and anger in patients with coronary heart disease.
Methods
The research method is quasi-experimental and pre-test-post-test and follow-up with the control group. The statistical population includes all patients with coronary heart disease (CHD) between 30 and 50 years old referred to medical centers and private clinics of cardiologists in Ilam in 2021, of which 60 people were available by random sampling and random distribution to three groups. Were divided. The data collection tool is the use of standard resilience and anger scales.
Results
The results of repeated measures analysis of variance showed the effectiveness of Gross model-based emotional regulation training in patient resilience, so that Benson muscle relaxation exercises compared to Gross model-based emotional regulation training increased patients' resilience and effect. It was better. Also, Benson muscle relaxation exercises were effective in reducing patients' anger. However, the results showed the ineffectiveness of Gross model-based emotion regulation training on anger.
Conclusions
By using Gross model emotion regulation training and Benson muscle relaxation exercises, we can take steps to provide services to patients with or with a history of heart disease and reduce the severity of the disease.
Evaluation of current Allied Health Professional (AHP) preceptorship programmes: a mixed method UK study
Authors: Salt Emma , Jackman Karen , O'Brien Anne
Published: July 2022

Aim: This study aimed to evaluate current preceptorship provision across AHP professions in the Staffordshire, Stoke on Trent (SSOT) region of England to improve consistency, share and optimise best practice. Background: Preceptorship, defined as a period of structured transition from newly qualified to an independent practitioner, is thought to improve recruitment and retention of staff and ultimately improve patient care. During the COVID pandemic SSOT recognised a particular need to support newly qualified staff, but were uncertain of existing AHP provision for recent graduates. Methods: An explanatory sequential mixed methods design, utilising a cross sectional survey questionnaire and two subsequent focus groups were used to explore existing AHP preceptorship in SSOT in 2021.Following ethical approval respondents were recruited via professional networks. Survey data were collected. Two subsequent focus groups enabled an in-depth exploration. Descriptive statistics were used to summarise survey data and thematic analysis to describe focus group findings. Results: 217 SSOT AHPs (26% preceptees; 47% preceptors) participated in the survey questionnaire and 20 in the focus groups. 57% of preceptees rated existing preceptorship programmes to be “somewhat or not effective”. Preceptors reported feeling unprepared for their role. Preceptees and preceptors reported that, post pandemic, most existing programs were no longer fit for purpose. Six pragmatic summary recommendations were made.Conclusions: AHP Preceptorship in SSOT was found to be inconsistent, poorly understood and inadequate. Revisions to SSOT preceptorship programs are planned to reflect changes in AHP practice since the COVID pandemic.
Interleukin-10 is not associated with obstructive sleep apnea hypopnea syndrome: a systematic review and Meta-analysis
Authors: Li Xiaoyan , Wu Lingyun , He Jie , Sun Qiuhua
Published: July 2022

Background
This study was conducted to explore the potential relationship between interleukin-10 (IL-10) and obstructive sleep apnea hypopnea syndrome (OSAHS).
Methods
All the related research articles published before May 2022 were retrieved through the online database (EMBASE, VIP, Wanfang, Web of Science, PubMed, and CNKI). Stata 11.0 software was used to calculate the standard mean difference (SMD) of the continuous variable and 95% confidence interval (CI). Expression profiles GSE38792 and GSE135917 were acquired from Gene Expression Omnibus (GEO) database, respectively. The expression of IL-10 mRNA in subcutaneous adipose tissue and visceral adipose tissue of obstructive sleep apnea-hypopnea syndrome (OSAHS) patients and healthy subjects was extracted by R software to verify the difference in IL-10 between the two groups.
Results
The IL-10 level in the plasma of people with and without OSAHS (STD Mean Difference (SMD) = -0.68, 95% CI = -1.58 ~ 0.21, I2 = 94.3%, P = 0.136) was the same. There was also no difference in IL-10 levels in serum between people with and without OSAHS (SMD = -0.12, 95% CI = -0.55 to 0.32, I2 = 94.4%, P = 0.591). In addition, the subjects were divided into different subgroups for meta-analysis according to race, body mass index, age, study type, and disease severity. Based on the outcomes, no notable difference was observed in the plasma/serum IL-10 level between the OSAHS subgroups and the control group. The results of bioinformatics analysis indicated that there was no significant difference in the expression of IL-10 mRNA in subcutaneous adipose tissue and visceral adipose tissue between patients with OSAHS and those in the control group.
Conclusion
the current meta-analysis highlighted that IL-10 levels between patients with OSAHS and healthy people had no difference.
A Stress Factor for Vitality and Nature: The Effect of Sodium Pyrithione on ACTH and CORT Levels in Rats
Authors: Gök Tuba , Kankılıç Tolga , Polat Hüseyin
Published: July 2022

The effects of sodium pyrithione (35 ve 70 mg/kg, ip) and saline (%0.09 NaCl 0.5 ml/kg, ip) administered intraperitoneally to rats on hormones after 24 and 96 hours were investigated. Due to sodium pyrithione (35 and 70 mg/kg, ip) administered to rats, the increase in ACTH and corticosterone levels in 24 hours was significant (p < 0.05), while the change was insignificant after 96 hours of administration. As a result of the research, it is thought that sodium pyrithione administered intraperitoneally to rats causes stress within 24 hours and leads to hormonal adaptation for survival.
Evaluation of granular insecticides against white grubs (Coleoptera: Scarabaeidae) infesting soybean crop
Authors: Kumar Sushil , Pandey Ajay Kumar
Published: July 2022

The experiment was carried out to study the efficacy of various insecticides viz., Carbofuran 3G @ 750g a.i./ha, Clothianidin 50WDG @ 120g a.i./ha, Fipronil 0.3G @ 50g a.i./ha, Thiamethoxam 25WG @ 80g a.i./ha, Imidacloprid 70WG @ 300g a.i./ha, Chlorantraniliprole 0.4 GR @100g a.i./ha, Fipronil 40% + Imidacloprid 40% WG @ 300g a.i./ha Chlorpyrifos 10G @ 300g a.i./ha and Emamectin benzoate 5SG @ 12.5g a.i./ha against white grubs infesting soybean crops. All treatments were shown to be significantly superior to the control. During the 2019 and 2020 cropping seasons, Fipronil 40% + Imidacloprid 40% WG @ 300g a.i./ha was shown to be the most effective in terms of percent decrease over the control, i.e., 81.95 and 79.31%, respectively, followed by Clothianidin 50WDG @ 120g a.i./ha (78.95 and 77.46%). Emamectin benzoate 5SG at 12.5g a.i./ha was found to be less effective, reducing plant damage by 30.07% during the previous year and 39.37% during the next years. The maximum soybean yield and cost benefit ratio was obtained from the plot treated with Fipronil 40% + Imidacloprid 40% WG @ 300g a.i./ha, while the lowest was obtained from the plot treated with Emamectin benzoate 5SG @ 12.5g a.i./ha. Observations recorded on the following application of insecticides revealed that there was no evidence of phytotoxicity in any of the treated soybean plots. Experimental results demonstrated that Fipronil 40% + Imidacloprid 40% WG applied at 300g a.i./ha was effective for the control of white grub in soybean crop without harming natural enemies.