bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus
Authors: Liu Hua , Chai Yuhong , Chen Haixia , Chen Wendan , Li Yushu , Liu Wenchao , Guo Shuang , Sun Lei , Zhou Xiumei , Huang Conglin , Tang Xiaowei , Luo Chang , Chen Dongliang , Cheng Xi
Published: September 2022

Opisthopappus longilobus, which is a unique wild plant resource in China, produces leaves and flowers with distinct aromas. However, there have been relatively few molecular studies on its floral aroma, which has hindered the research on this plant species at the molecular level and the breeding of novel varieties. In this study, transcriptome and metabolome analyses were performed using O. longilobus leaves, buds, and inflorescences at the exposure, initial opening, and blooming stages. Using high-quality reads and assembly software, a total of 45,674 unigenes were annotated according to the Nr, Swiss-Prot, KOG, and KEGG databases. Additionally, a GC-MS system and a self-built database were used to detect 1,371 metabolites in the leaves, buds, and inflorescences. Terpene metabolites were the most common compounds (308 in total). We analyzed the gene network regulating terpenoid accumulation in O. longilobus and identified 56 candidate genes related to terpenoid synthesis. The expression of OlPMK2, OlMVK1, OlTPS1, and OlTPS3 may lead to the accumulation of 11 different terpenoids specifically in the inflorescences at the exposure, initial opening, and blooming stages. The generated data may be useful for future research on O. longilobus genetic resources and the molecular mechanism regulating aroma formation in this plant species. The findings of this study may be used to accelerate the breeding of new O. longilobus varieties with enhanced aromatic traits.
Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheat
Authors: Wang Huifang , Han Xiaofan , Fu Xiaofeng , Sun Xinling , Chen Hailong , Wei Xirui , Cui Shubin , Liu Yiguo , Guo Weiwei , Li Ximei , Xing Jiewen , Zhang Yumei
Published: September 2022

Lateral organ boundaries domain (LBD) proteins, a class of plant-specific transcription factors with a special domain of lateral organ boundaries (LOB), play essential roles in plant growth and development. However, there is little known about the functions of these genes in wheat to date. Our previous study demonstrated that TaLBD16-4D is conducive to increasing lateral root number in wheat. In the present work, we further examined important agronomical traits of the aerial part of transgenic wheat overexpressing TaLBD16-4D. Interestingly, it was revealed that overexpressing TaLBD16-4D could lead to early heading and multiple alterations of plant architecture, including decreased plant height, increased flag leaf size and stem diameter, reduced spike length and tillering number, improved spike density and grain width, and decreased grain length. Moreover, auxin-responsive experiments demonstrated that the expression of TaLBD16-4D in wild-type (WT) wheat plants showed a significant upregulation through 2,4-D treatment. TaLBD16-4D-overexpression lines displayed a hyposensitivity to 2,4-D treatment and reduced shoot gravitropic response. The expressions of a set of auxin-responsive genes were markedly different between WT and transgenic plants. In addition, overexpressing TaLBD16-4D affected the transcript levels of flowering-related genes (TaGI, TaCO1, TaHd1, TaVRN1, TaVRN2, and TaFT1). Notably, the expression of TaGI, TaCO1, TaHd1, TaVRN1, and TaFT1 displayed significant upregulation under IAA treatment. Collectively, our observations indicated that overexpressing TaLBD16-4D could affect aerial architecture and heading time possibly though participating in the auxin pathway.
Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato
Authors: Liu Danmei , Li Min , Guo Ting , Lu Juanjuan , Xie Yafang , Hao Yuan , Wang Longdan , Zhao Dan , Zhang Liping , Liu Zhiqiang , Jin Zhuping , Pei Yanxi
Published: September 2022

Sulfur-containing compounds are essential for plant development and environmental adaptation, and closely related to the flavor and nutrition of the agricultural products. Cysteine, the first organic sulfur-containing molecule generated in plants, is the precursor for most of these active substances. Serine acetyltransferase (SERAT) catalyzes the rate-limiting step of its formation. However, despite their importance, systematic analyses of these enzymes in individual species, especially in economically important crops, are still limited. Here, The SERAT members (SlSERATs, four in total) were identified and characterized in tomato. Phylogenetically, the four SlSERAT proteins were classified into three subgroups with distinct genomic structures and subcellular localizations. On the function, it was interesting to find that SlSERAT3;1, possessed a high ability to catalyze the formation of OAS, even though it contained a long C-terminus. However, it retained the essential C-terminal Ile, which seems to be a characteristic feature of SERAT3 subfamily members in Solanaceae. Besides, SlSERAT1;1 and SlSERAT2;2 also had high activity levels and their catalyzing abilities were significantly improved by the addition of an OAS-(thiol)-lyase protein. At the transcriptional level, the four SlSERAT genes had distinct expression patterns during tomato plant development. Under abiotic stress conditions, the chloroplast-localized SlSERATs were the main responders, and the SlSERATs adopted different strategies to cope with osmotic, ion toxicity and other stresses. Finally, analyses in the loss-of-function and overexpression lines of SlSERAT1;1 suggested that function redundancy existed in the tomato SERAT members, and the tomato SERAT member was ideal target for S-assimilation manipulating in molecular breeding.
Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L
Authors: Lin Qi , Li Miaomiao , Wang Ying , Xu Zhixia , Li Lei
Published: September 2022

Rhizosphere bacterial diversity and community structure are important factors involving in plant growth. However, the exact process of how plant rhizosphere bacterial community structures is assembled remains unclear. To investigate the role of bacterial chemotaxis to rhizosphere secretions in the establishment of rhizosphere microbial community in Casuarina equisetifolia, we screened bacteria strains derived from the rhizosphere of Casuarina equisetifolia L. using top three chemicals of the plant root exudates (2,4-di-tert-butylphenol, methyl stearate, and arginine) as chemoattractant. Among 72 bacterial strains, five showed strong chemotaxis to 2,4-di-tert-butylphenol, six to methyl stearate, and eleven to arginine, with the highest bacterial chemotaxis occurring at a concentration of 60 μM. This indicates that arginine is a more important chemoattractant than 2,4-di-tert-butylphenol, methyl stearate in the establishment of rhizosphere microbial community in Casuarina equisetifolia. Bacterial community assembly analysis using different chemoattractants and chemoattractants-plus-bacteria combinations were then performed by burying laboratory prepared bags of sterlized soil into C. equisetifolia forest. Bacteria diversity and enrichment analyses using 16S rDNA sequencing at 7 and 14 days after burying showed that arginine-plus-Ochrobactrum sp. and Pantoea sp. treatment exhibited the greatest similarity to the natural forest bacterial community. Our date provides new insights into how chemoattractants and chemotactic bacteria strains shape the rhizosphere microbial community of C. equisetifolia, which constitutes foundational information for future management of these communities.
Plant defense: ARR11 response regulator as a potential player in Arabidopsis
Authors: Falconieri Gaia Salvatore , Bertini Laura , Bizzarri Elisabetta , Proietti Silvia , Caruso Carla
Published: September 2022

Plant growth and response to environmental cues are largely driven by hormones. Salicylic acid (SA)- and jasmonic acid (JA)-mediated defenses have been shown to be effective against different types of attackers. SA-mediated defense is mainly effective against biotrophic pathogens and phloem-feeding insects, whereas JA-mediated defense is effective against necrotrophic pathogens and tissue-damaging insects. Cytokinins (CKs) are classic growth hormones that have also emerged as plant immunity modulators. Evidence pointed out that CKs contribute to the defense responses mediated by SA and JA, acting as hormone modulators of the SA/JA signaling backbone. Recently, we identified in Arabidopsis a type-B response regulator 11 (ARR 11) involved in cytokinin-mediated responses as a novel regulator of the SA/JA cross-talk. Here we investigated plant fitness and resistance against the fungal necrotrophic pathogen Botrytis cinerea in Arabidopsis wild-type Col-8 and defective arr11 mutant following SA, JA, CK single or combined treatment. Our results demonstrated that the CK and SA/JA/CK combination has a positive outcome on plant fitness in both Arabidopsis Col-8 and arr11 mutant,. The triple hormone treatment is efficient in increasing resistance to B. cinerea in Col-8 and this effect is stronger in arr11 mutant. The results will provide not only new background knowledge, corroborating the role of ARR11 in plant-defense related processes, but also new potential opportunities for alternative ways of protecting plants from fungal diseases.
Predictive analysis of the psychological state of charismatic leaders on employees' work attitudes based on artificial intelligence affective computing
Authors: Liu Yi , Song Jaehoon
Published: September 2022

With the progress of social production, the competition for talents among enterprises is fierce, and the market often lacks capable leaders, which leads to the lack of management of enterprise employees and cannot bring more economic benefits to enterprises. Traditional leaders make subordinate employees work actively and achieve the common goal of the enterprise by exerting their own leadership characteristics and observing their subordinates, but they cannot take care of the psychological state of each employee, resulting in the employee's work efficiency is not very high. In recent years, charismatic leadership has become an important economic leader in the new era, and the theoretical spirit of charismatic leadership can well guide employees to work actively. Artificial intelligence affective computing can well identify the psychological state of the subject, and the combination of artificial intelligence affective computing and charismatic leadership can achieve effective management of employees through the predictive analysis of employees' psychological state. This paper compares the psychological state prediction analysis of employees' work attitudes between charismatic leaders based on artificial intelligence affective computing and traditional leaders through experiments. The results show that: charismatic leaders based on artificial intelligence affective computing predictive analytics can improve sensitivity to employee needs, resulting in an 8.2% increase in employee trust in leadership, a 4.4% increase in employee commitment to achieving organizational goals, and a 19.3% increase in employee job satisfaction. The psychological state prediction analysis of charismatic leaders based on artificial intelligence affective computing on employees' work attitudes can improve the work efficiency of employees and the economic benefits of enterprises.
The impact of follower leadership position on transformational leadership as moderator of the association between work-related ambiguity and job satisfaction
Authors: Nielsen Morten Birkeland , Hetland Jørn , Harris Anette , Notelaers Guy , Gjerstad Johannes , Einarsern Ståle Valvatne
Published: September 2022

This two-part study examined if the buffering effect of transformational leadership on the association between work-related ambiguity and job satisfaction is contingent upon whether a follower holds a formal leadership position him/herself. Data from two separate surveys were employed: Study 1: A sample of 845 respondents from Belgium. Study 2: A national probability sample of 1,608 Norwegian employees. Study 1 showed that task ambiguity had a significant negative relation with job satisfaction, but that transformational leadership did only buffer the association between task ambiguity and job satisfaction among employees holding a formal position as a supervisor or manager. Study 2 extended Study 1 by adjusting for age and job tenure of subordinates as a confounding variable. Study 2 confirmed that transformational leadership had a significantly stronger impact on the observed association between role ambiguity and job satisfaction among respondents holding a supervisor or manager position. In conclusion, when considering job satisfaction as an outcome of work-related ambiguity, transformational leadership is mainly beneficial for followers holding a formal supervisor or manager position themselves. Our findings thereby question assumptions about the general effectiveness of transformational leadership.
Preschool children’s negative affect and social skills in China: The moderating effect of the teacher–child relationship
Authors: Wang Yingjie , Tao Ying , Zhu Li , Li Yan , Huang Dingwen
Published: September 2022

Negative affect is an important temperament in children, influencing their social skills. However, the evidence for this association in preschool children is limited. Moreover, the underlying mechanisms that explain the relationship between preschool children’s negative affect and social skills remain less understood. Thus, the primary goal of this study was to examine the moderating role of the teacher–child relationship in the associations between negative affect and social skills of Chinese preschoolers. A sample of 198 preschool children (Mage = 58.64 ± 3.83 months, 53% boys), recruited from 13 classes in two public kindergartens in Shanghai, China, participated in this 1-year longitudinal study. The mothers reported children’s negative affect, while the teachers reported children’s social skills and the teacher–child relationship. Results of a series of moderating effect analyses showed that (1) T1 negative affect could significantly negatively predict T2 social skills (cooperation, self-control, and assertion), and (2) the associations between negative affect and social skills were moderated by the teacher–child relationship (including teacher–child closeness and conflict). Specifically, a high level of teacher–child closeness buffered the prediction of negative affect on social skills, while a high level of teacher–child conflict aggravated the said prediction. The findings highlight the importance of the teacher–child relationship in the development of children’s social skills during preschool age when they have a high level of negative affect. This has important implications for the development of interventions to improve teacher–child relationships and children’s social skills.
Believing processes around COVID-19 vaccination: An exploratory study investigating workers in the health sector
Authors: Fleischmann Eva , Fellendorf Frederike , Schönthaler Elena M. , Lenger Melanie , Hiendl Lena , Bonkat Nina , Wagner-Skacel Jolana , Bengesser Susanne , Angel Hans-Ferdinand , Seitz Rüdiger J. , Reininghaus Eva Z. , Dalkner Nina
Published: September 2022

Background

The processes underlying believing have been labeled “creditions”, which are important brain functions between emotion and cognition. Creditions are influenced by both internal and external factors, one of which is the coronavirus disease 2019 (COVID-19) pandemic and the vaccination against the disease.

Methods

To investigate believing processes shortly before the implementation of a mandatory vaccination in Austria, both vaccinated and unvaccinated workers in the health sector (WHS) were surveyed in December 2021/January 2022. In total, 1,062 vaccinated and 97 unvaccinated WHS (920 females) completed the online survey. Beliefs were assessed using the parameters of the credition model (narrative, certainty, emotion, and mightiness) with regard to (1) the COVID-19 pandemic in general, and (2) the vaccination. Type of emotion and narrative were divided into positive, negative, and indifferent. Moreover, the congruence between emotion and narrative was calculated.

Results

The vaccination rate of the sample was 91.6%, with a significantly higher percentage of men being in the group of vaccinated (21.1%) as compared to unvaccinated individuals (12.4%). Pertaining beliefs about the COVID-19 vaccination, unvaccinated WHS reported more negative and less positive emotions as well as content of narrative than vaccinated WHS. In addition, they showed higher levels of certainty as well as mightiness while believing and felt less sufficiently informed about governmental and workplace-related COVID-19 measures. The groups did not differ in the type of emotion or content of narrative in their beliefs about the pandemic in general.

Conclusion

In conclusion, unvaccinated WHS had more negative and less positive emotions and thoughts than vaccinated WHS in their beliefs about the COVID-19 vaccination and their motivations for not having received it. They were more certain about their beliefs and felt stronger negative emotions in their beliefs compared to vaccinated individuals. Providing unvaccinated WHS with adequate information might be helpful in reducing their mental burden.
Diagnostic performance of adenosine deaminase for abdominal tuberculosis: A systematic review and meta-analysis
Authors: Zhou Ruixi , Qiu Xia , Ying Junjie , Yue Yan , Ruan Tiechao , Yu Luting , Liu Qian , Sun Xuemei , Wang Shaopu , Qu Yi , Li Xihong , Mu Dezhi
Published: September 2022

Background and aim

Abdominal tuberculosis (TB) is a common type of extrapulmonary TB with an insidious onset and non-specific symptoms. Adenosine deaminase (ADA) levels increase rapidly in the early stages of abdominal TB. However, it remains unclear whether ADA serves as a diagnostic marker for abdominal TB.

Methods

We performed a systematic literature search for relevant articles published in PubMed, Web of Science, Cochrane Library, and Embase up to April 2022. First, we used the Quality Assessment of Diagnostic Accuracy Studies tool-2 (QUADAS-2), to evaluate the quality of the included articles. Bivariate and hierarchical summary receiver operating characteristic (HSROC) models were then utilized to analyze pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the receiver operating characteristic curve (AUROC). In addition, we explored a subgroup analysis for potential heterogeneity and publication bias among the included literature.

Results

Twenty-four articles (3,044 participants, 3,044 samples) which met the eligibility criteria were included in this study. The pooled sensitivity and specificity of ADA for abdominal TB detection were 93% [95% confidence interval (CI): 0.89–0.95] and 95% (95% CI: 0.93–0.96), respectively. PLR and NLR were 18.6 (95% CI: 14.0–24.6) and 0.08 (95% CI: 0.05–0.12), respectively. DOR and AUROC were 236 (95% CI: 134–415) and 0.98 (95% CI: 0.96–0.99), respectively. Furthermore, no heterogeneity or publication bias was found.

Conclusions

Our meta-analysis found ADA to be of excellent diagnostic value for abdominal TB and could be used as an auxiliary diagnostic tool.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier: CRD42022297931.
Co-creating physical activity interventions: Findings from a multiple case study using mixed methods
Authors: Popp Johanna , Grüne Eva , Carl Johannes , Semrau Jana , Pfeifer Klaus
Published: September 2022

Introduction

In health and physical activity promotion, there is growing interest in co-creation approaches that involve researchers and non-academic stakeholders in developing new interventions. Previous research has shown the promising results of cooperative planning as a co-creation approach in building new capacities and implementing physical activity-promoting interventions in nursing care and automotive mechatronics. However, it remains unclear whether (1) cooperative planning for physical activity promotion can be successfully transferred to other settings in the nursing care and automotive mechatronic sectors and (2) what key factors influence its success or failure.

Methods

We conducted a multiple case study in three settings in the nursing care and automotive mechatronics sectors. Following a mixed methods approach, we collected, analyzed, and triangulated data from documents (n= 17), questionnaires (n= 66), and interviews (n= 6). Quantitative data were analyzed descriptively and through using nonparametric analyses of variance; qualitative data were analyzed using qualitative content analysis by extraction.

Results

The transfer of cooperative planning to new settings was realized, though the impact varied by setting. While the interventions were developed and implemented in nursing care settings, interventions were developed but not implemented in the automotive mechatronics setting. In this context, intervention implementation was influenced by 11 key factors:champion, commitment, embedment, empowerment, engagement, health-promoting leadership, ownership, relevance, resources, responsibility, andstrategic planning. Furthermore, the transfer of cooperative planning was influenced by different activity characteristics, namelyelaboration & reconsideration, group composition, number of meetings, participation, period, prioritization, andresearchers' input & support.

Discussion

The present article contributes to a better understanding of a co-creation approach utilized for physical activity promotion and provides new insights into (1) the transferability of cooperative planning and (2) the associated key factors influencing intervention implementation. The success of cooperative planning varied by setting and was influenced by several activity characteristics and key factors, some of which showed complex relationships. This raises the question of whether some settings might benefit more from a co-creation approach than others. Therefore, future co-creation initiatives should carefully consider the specific characteristics of a setting to select and apply the most appropriate approach.
Cost-effectiveness of ensartinib, crizotinib, ceritinib, alectinib, brigatinib and lorlatinib in patients with anaplastic lymphoma kinase-positive non-small cell lung cancer in China
Authors: Luo Xia , Zhou Zhen , Zeng Xiaohui , Peng Liubao , Liu Qiao
Published: September 2022

Objective

Six anaplastic lymphoma kinase-tyrosine kinase inhibitors (ALK-TKIs), including one domestic (ensartinib) and five imported ALK-TKIs (crizotinib, ceritinib, alectinib, brigatinib, and lorlatinib), have been recommended as first-line treatments for advanced ALK-positive NSCLC in China. This study sought to examine the cost-effectiveness of these six novel therapies in Chinese patients.

Material and methods

We constructed a Markov model to compare the cost-effectiveness of the six ALK-TKIs as a first-line treatment for patients with advanced ALK-positive NSCLC from the perspective of the Chinese healthcare system. Transition probabilities were estimated by synthesizing data from the PROFILE 1,029 trial and a network meta-analysis. Health state utilities and costs were sourced from published literature, publicly available national databases, and local general hospitals. The robustness of model was assessed via deterministic sensitivity analyses and probabilistic sensitivity analyses.

Results

Compared with crizotinib, ensartinib achieved additional 0.12 quality-adjusted life-year (QALY) with marginal costs of $3,249, resulting in an incremental cost-effectiveness ratio (ICER) of $27,553/ QALY. When compared with ceritinib and brigatinib, ensartinib achieved additional 0.06 and 0.03 QALYs with substantially reduced costs. When compared with lorlatinib and alectinib, ensartinib was associated with a lower QALY and decreased total costs; the ICERs for lorlatinib and alectinib were $934,101/ QALY and $164,888/ QALY, respectively.

Conclusion

For Chinese patients with advanced ALK-positive NSCLC, ensartinib was a cost-effective option compared with crizotinib, and was a dominant alternative to ceritinib and brigatinib. Although lorlatinib and alectinib were associated with prolonged survival compared with ensartinib, they were less cost-effective than ensartinib due to the overwhelming total costs.
Magnetic field properties in star formation: A review of their analysis methods and interpretation
Authors: Liu Junhao , Zhang Qizhou , Qiu Keping
Published: September 2022

Linearly polarized emission from dust grains and molecular spectroscopy is an effective probe of the magnetic field topology in the interstellar medium and molecular clouds. The longstanding Davis-Chandrasekhar-Fermi (DCF) method and the recently developed Histogram of Relative Orientations (HRO) analysis and the polarization-intensity gradient (KTH) method are widely used to assess the dynamic role of magnetic fields in star formation based on the plane-of-sky component of field orientations inferred from the observations. We review the advances and limitations of these methods and summarize their applications to observations. Numerical tests of the DCF method, including its various variants, indicate that its largest uncertainty may come from the assumption of energy equipartition, which should be further calibrated with simulations and observations. We suggest that the ordered and turbulent magnetic fields of particular observations are local properties of the considered region. An analysis of the polarization observations using DCF estimations suggests that magnetically trans-to-super-critical and averagely trans-to-super-Alfvénic clumps/cores form in sub-critical clouds. High-mass star-forming regions may be more gravity-dominant than their low-mass counterparts due to higher column density. The observational HRO studies clearly reveal that the preferential relative orientation between the magnetic field and density structures changes from parallel to perpendicular with increasing column densities, which, in conjunction with simulations, suggests that star formation is ongoing in trans-to-sub-Alfvénic clouds. There is a possible transition back from perpendicular to random alignment at higher column densities. Results from observational studies using the KTH method broadly agree with those of the HRO and DCF studies.
What aspects of solar flares can be clarified with mm/submm observations?
Authors: Fleishman Gregory D. , Martinez Oliveros Juan Carlos , Landi Enrico , Glesener Lindsay
Published: September 2022

This paper identifies several unsolved questions about solar flares, which can potentially be answered or at least clarified with mm/submm observations with ALMA. We focus on such questions as preflare phases and the initiation of solar flares and the efficiency of particle acceleration during flares. To investigate the preflare phase we propose to use the extraordinary sensitivity and high spatial resolution of ALMA, which promises to identify very early enhancements of preflare emission with high spatial resolution and link them to the underlying photospheric magnetic structure and chromospheric flare ribbons. In addition to revealing the flare onsets, these preflare measurements will aid in the investigation of particle acceleration in multiple ways. High-frequency imaging spectroscopy data in combination with the microwave data will permit the quantification of the high-energy cutoff in the nonthermal electron spectra, thus helping to constrain the acceleration efficiency. Detection and quantification of secondary relativistic positron (produced due to nonthermal accelerated ions) contribution using the imaging polarimetry data will help constrain acceleration efficiency of nonthermal nuclei in flares. Detection of a “mysterious” rising spectral component with high spatial resolution will help determine the emission mechanism responsible for this component, and will then help in quantifying this either nonthermal or thermal component of the flaring plasma. We discuss what ALMA observing mode(s) would be the most suitable for addressing these objectives.
The tibial insertion of the hamstring can be considered to be preserved during anterior cruciate ligament reconstruction
Authors: Fang Kaibin , Dai Zhangsheng , Lin Xiaocong
Published: September 2022

Background

Hamstring as a graft was very common in anterior cruciate ligament reconstruction surgery. Usually the hamstring muscles needed to be taken out and then woven to be used.

Aim

In order to investigate whether it was beneficial for patients to preserve the transpedicular insertion of hamstring when using the hamstring as a graft for anterior cruciate ligament reconstruction.

Methods

This was a retrospective study. Patients with anterior cruciate ligament injury who underwent surgery in a large hospital from January 2015 to May 2021 were included in the study. These patients underwent anterior cruciate ligament reconstruction assisted by arthroscopic. Autologous hamstring muscles were used as grafts. The tibial insertion of the hamstring were preserved during the operation were included in the observation group. The remaining patients were included in the control group. The knee joint function and operation of the two groups were compared.

Results

A total of 97 patients were included in the study. There was no statistical difference between the two groups in general data including gender, age and surgical side. All the patients’ operations were successfully completed there was no significant difference in the operation time between the two groups. All patients were followed up for at least 1 year. No patients had complications such as wound infection and graft failure at the last follow-up. There was no significant difference between the two groups in Lysholm score and IKDC score before operation. Similarly, there was no significant difference between the two groups in Lysholm score and IKDC score 3 months after operation. However, the Lysholm score and IKDC score of the two groups 1 year after operation were statistically different, and the patients in the observation group had higher Lysholm score and IKDC score. After comparing the MRI images of the knee of the two groups 3 months after operation through the MRI evaluation system, compared with the patients in the control group, the patients in the observation group have higher scores, and the difference was statistically significant.

Conclusion

In the knee arthroscopic assisted anterior cruciate ligament reconstruction using the hamstring as a graft, the tibial insertion of the hamstring can be preserved, which can make the patient have better function after the operation. This kind of operation leads to the increase of operation time and operation risk.
Case Report: Oral Fecal Microbiota Transplantation in a Dog Suffering From Relapsing Chronic Diarrhea—Clinical Outcome and Follow-Up
Authors: Cerquetella Matteo , Marchegiani Andrea , Rossi Giacomo , Trabalza-Marinucci Massimo , Passamonti Fabrizio , Isidori Marco , Rueca Fabrizio
Published: September 2022

The present case report describes the effects of orally administered fecal microbiota transplantation (FMT) (frozen capsules) in a dog suffering from relapsing chronic diarrhea, needing a continuous low prednisolone dose to maintain the condition under acceptable control. Through FMT, we aimed at evaluating the possibility of improving the clinical score and/or reducing/suspending steroid administration. During a first period of strict monitoring (21 days), the canine inflammatory bowel disease activity index (CIBDAI) score passed from mild to clinically insignificant disease. Furthermore, two additional gastrointestinal signs that had been reported, bloating and episodes of painful defecation, rapidly improved (bloating) or even resolved (painful defecation). The patient was then followed for 18 months (to the authors' knowledge, the longest follow-up time ever reported in a dog), during which no serious relapses occurred and no increase in prednisolone dose was necessary. No adverse clinical effects were ever reported during monitoring. The present description provides a further experience increasing those already present in the veterinary literature, in which an agreement on how to use FMT has not yet been achieved although strongly needed and recommended.
Canine chronic enteropathy—Current state-of-the-art and emerging concepts
Authors: Jergens Albert E. , Heilmann Romy M.
Published: September 2022

Over the last decade, chronic inflammatory enteropathies (CIE) in dogs have received great attention in the basic and clinical research arena. The 2010 ACVIM Consensus Statement, including guidelines for the diagnostic criteria for canine and feline CIE, was an important milestone to a more standardized approach to patients suspected of a CIE diagnosis. Great strides have been made since understanding the pathogenesis and classification of CIE in dogs, and novel diagnostic and treatment options have evolved. New concepts in the microbiome-host-interaction, metabolic pathways, crosstalk within the mucosal immune system, and extension to the gut-brain axis have emerged. Novel diagnostics have been developed, the clinical utility of which remains to be critically evaluated in the next coming years. New directions are also expected to lead to a larger spectrum of treatment options tailored to the individual patient. This review offers insights into emerging concepts and future directions proposed for further CIE research in dogs for the next decade to come.
Zooming in on the molecular characteristics of swine influenza virus circulating in Colombia before and after the H1N1pdm09 virus
Authors: Osorio-Zambrano William F. , Ospina-Jimenez Andres F. , Alvarez-Munoz Santiago , Gomez Arlen P. , Ramirez-Nieto Gloria C.
Published: September 2022

Influenza is one of the most critical viral agents involved in the respiratory disease complex affecting swine production systems worldwide. Despite the absence of vaccination against swine influenza virus in Colombia, the serologic reactivity to classic H1N1 and H3N2 subtypes reported since 1971 indicates the virus has been circulating in the country's swine population for several decades. However, successful isolation and sequencing of field virus from pigs was nonexistent until 2008, when H1N1 classical influenza virus was identified. One year later, due to the emergence of the influenza A (H1N1) pdm09 virus, responsible for the first global flu pandemic of the 21st century, it was introduced in the country. Therefore, to understand the impact of the introduction of the H1N1pdm09 virus in Colombia on the complexity and dynamics of influenza viruses previously present in the swine population, we carried out a study aiming to characterize circulating viruses genetically and establish possible reassortment events that might have happened between endemic influenza viruses before and after the introduction of the pandemic virus. A phylogenetic analysis of ten swine influenza virus isolates from porcine samples obtained between 2008 and 2015 was conducted. As a result, a displacement of the classical swine influenza virus with the pdmH1N1 virus in the swine population was confirmed. Once established, the pandemic subtype exhibited phylogenetic segregation based on a geographic pattern in all the evaluated segments. The evidence presents reassortment events with classic viruses in one of the first H1N1pdm09 isolates. Thus, this study demonstrates complex competition dynamics and variations in Colombian swine viruses through Drift and Shift.
Leukemia inhibitory factor enhances the development and subsequent blastocysts quality of yak oocytes in vitro
Authors: Zhao Tian , Pan Yangyang , Li Qin , Ding Tianyi , Niayale Robert , Zhang Tongxiang , Wang Jinglei , Wang Yaying , Zhao Ling , Han Xiaohong , Baloch Abdul Rasheed , Cui Yan , Yu Sijiu
Published: September 2022

Leukemia inhibitory factor (LIF) is a multipotent cytokine of the IL-6 family which plays a critical role in the maturation and development of oocytes. This study evaluated the influence of LIF on the maturation and development ability of yak oocytes, and the quality of subsequent blastocysts under in vitro culture settings. Different concentrations of LIF (0, 25, 50, and 100 ng/mL) were added during the in vitro culture of oocytes to detect the maturation rate of oocytes, levels of mitochondria, reactive oxygen species (ROS), actin, and apoptosis in oocytes, mRNA transcription levels of apoptosis and antioxidant-related genes in oocytes, and total cell number and apoptosis levels in subsequent blastocysts. The findings revealed that 50 ng/mL LIF could significantly increase the maturation rate (p < 0.01), levels of mitochondria (p < 0.01) and actin (p < 0.01), and mRNA transcription levels of anti-apoptotic and antioxidant-related genes in yak oocytes. Also, 50 ng/mL LIF could significantly lower the generation of ROS (p < 0.01) and apoptosis levels of oocytes (p < 0.01). In addition, blastocysts formed from 50 ng/mL LIF-treated oocytes showed significantly larger total cell numbers (p < 0.01) and lower apoptosis rates (p < 0.01) than the control group. In conclusion, the addition of LIF during the in vitro maturation of yak oocytes improved the quality and the competence of maturation and development in oocytes, as well as the quality of subsequent blastocysts. The result of this study provided some insights into the role and function of LIF in vitro yak oocytes maturation, as well as provided fundamental knowledge for assisted reproductive technologies in the yak.
Corner Centrality of Nodes in Multilayer Networks: A Case Study in the Network Analysis of Keywords
Authors: Rodriguez-Sánchez Rosa María , Chamorro-Padial Jorge
Published: September 2022

In this paper, we present a new method to measure the nodes’ centrality in a multilayer network. The multilayer network represents nodes with different relations between them. The nodes have an initial relevance or importance value. Then, the node’s centrality is obtained according to this relevance along with its relationship to other nodes. Many methods have been proposed to obtain the node’s centrality by analyzing the network as a whole. In this paper, we present a new method to obtain the centrality in which, in the first stage, every layer would be able to define the importance of every node in the multilayer network. In the next stage, we would integrate the importance given by each layer to each node. As a result, the node that is perceived with a high level of importance for all of its layers, and the neighborhood with the highest importance, obtains the highest centrality score. This score has been named the corner centrality. As an example of how the new measure works, suppose we have a multilayer network with different layers, one per research area, and the nodes are authors belonging to an area. The initial importance of the nodes (authors) could be their h-index. A paper published by different authors generates a link between them in the network. The authors can be in the same research area (layer) or different areas (different layers). Suppose we want to obtain the centrality measure of the authors (nodes) in a concrete area (target layer). In the first stage, every layer (area) receives the importance of every node in the target layer. Additionally, in the second stage, the relative importance given for every layer to every node is integrated with the importance of every node in its neighborhood in the target layer. This process can be repeated with every layer in the multilayer network. The method proposed has been tested with different configurations of multilayer networks, with excellent results. Moreover, the proposed algorithm is very efficient regarding computational time and memory requirements.
Stability and Convergence Analysis of a Domain Decomposition FE/FD Method for Maxwell’s Equations in the Time Domain
Authors: Asadzadeh Mohammad , Beilina Larisa
Published: September 2022

Stability and convergence analyses for the domain decomposition finite element/finite difference (FE/FD) method are presented. The analyses are developed for a semi-discrete finite element scheme for time-dependent Maxwell’s equations. The explicit finite element schemes in different settings of the spatial domain are constructed and a domain decomposition algorithm is formulated. Several numerical examples validate convergence rates obtained in the theoretical studies.
Improving Agricultural Green Supply Chain Management by a Novel Integrated Fuzzy-Delphi and Grey-WINGS Model
Authors: Wang Muwen , Zhang Kecheng
Published: September 2022

This study suggests a novel hybrid model for calculating the interrelationships between factors by integrating the Fuzzy set, Delphi, the Grey theory, and Weighted Influence Nonlinear Gauge System (WINGS) approaches in agricultural green supply chain management (AGSCM). Fuzzy Delphi helps to select 12 indicators from 19 factors by defuzzification for ambiguity associated with subjective judgment by 10 experts in data collection. Grey WINGS can illustrate the relationships, direction, and strength of factors simultaneously, which illustrates that environmental law, green consciousness, product quality, and price are the most significant factors of AGSCM. The results can help operators not only to analyze these key influencing factors, but also to understand the complex cause-and-effect relationships between these factors. This integrated model will hopefully provide a useful tool to agricultural policy makers and decision makers for sustainable development.
Product Type, Rice Variety, and Agronomic Measures Determined the Efficacy of Enhanced-Efficiency Nitrogen Fertilizer on the CH4 Emission and Rice Yields in Paddy Fields: A Meta-Analysis
Authors: Yang Tong , Wang Mengjie , Wang Xiaodan , Xu Chunchun , Fang Fuping , Li Fengbo
Published: September 2022

Enhanced-efficiency nitrogen fertilizer (EENF) is a recommend nitrogen fertilizer for rice production because of its advantage on improving nitrogen use efficiency. However, its efficacy on CH4, the dominant greenhouse gas, emission from rice fields showed great variation under field conditions. And the factors influencing its efficacy are still unclear. We synthesized the results of 46 field studies and analyzed the impact of product type, rice variety, and primary agronomic measures (rice cropping system, nitrogen (N) application rate, and water management options) on the effectiveness of EENF on the CH4 emission and rice yield. Overall, EENF, including inhibitors (IS) and slow/control-released fertilizer (S/CRF), significantly reduced CH4 emission by 16.2% and increased rice yield by 7.3%, resulting in a significant reduction in yield-scaled CH4 by 21.7%, compared with conventional N fertilizer. Nitrapyrin, DMPP (3,4-dimethylpyrazole phosphate), and HQ (Hydroquinone) + Nitrapyrin showed relative higher efficacy on the mitigation of CH4 emission than other EENF products; and HQ showed relative lower efficacy on rice yield than other EENF products. The reduction in CH4 emission response of hybrid rice varieties to IS and S/CRF was greater than that of inbred rice varieties. IS significantly reduced the CH4 emission and increased the rice yield under all three rice cropping systems, and showed the highest efficacy in the late rice season of double rice cropping system. Whereas, S/CRF did not significantly reduce the CH4 emission from rice seasons of single rice cropping system and rice-upland crops rotation system. IS did not reduce the CH4 emission when N application rate less than 100 kg ha−1, and S/CRF did not affect the CH4 emission when N application rate less than 100 kg ha−1 or above 200 kg ha−1. Continuous flooding was unfavorable for IS and S/CRF to mitigate CH4 emission and enhance rice yield. These results emphasized the necessary to link EENF products with rice varieties and agronomic practices to assess their efficacy on CH4 emissions and rice yield.
Phenotypic Plasticity in Morphological Traits of Abelmoschus esculentus L. Induced by Histone Deacetylase Inhibitor, Trichostatin A
Authors: Sasikumar Sasipriya , Marulasiddappa Dushyanthakumar Banur , Sridhara Shankarappa , Adivappar Nagarajappa , Babu Bheemanapalli Nagraja Harish , M. El-Shehawi Ahmed , Aloufi Salman , Alqurashi Mohammed , Elansary Hosam O. , Elhindi Khalid M. , Mahmoud Eman A.
Published: September 2022

Epigenetic changes such as DNA methylation and histone modifications, when meiotically inherited across generations, can act as a stable evolutionary force that is independent of any accompanying DNA mutations. Certain histone deacetylase (HDAC)-inhibiting chemicals such as Trichostatin A (TSA) and sodium butyrate are known to regulate the total acetylated histones in the genome, which is important for regulating the expression of various traits in all organisms. This study investigated all morphological variations in traits of Abelmoschus esculentus L. (okra) induced by different doses of Trichostatin A in a popular okra variety, Arka Abhay. Two sets of seeds were treated with two doses (0.4 µM and 1.2 µM) of TSA and were incubated in the chemical for three and five days, respectively, to record the effects of dose and incubation periods on various agronomic characters of okra. The treatment of TSA had a negative impact on the majority of the characters under evaluation. Total seedlings emerged, and mean shoot and root length were retarded following the TSA treatment. Extremely dwarfed plants with malformed leaves and flowers were a common observation. Pollen sterility combined with distortion of the reproductive whorls of the flowers were particularly pronounced at high doses with a prolonged incubation period. Treated plants had a significantly delayed first flowering and produced short fruits with altered morphology. Variations in seeds with respect to the number, colour and size were also recorded. Total reduction with respect to seedling parameters, total pollen production, the number of fertile pollens, plant height and other damaging effects on leaves, flowers, fruits and seeds increased as the dose and incubation period increased. Statistical analysis revealed the significant negative effect of TSA treatments on plant height, the number of ridges and locules per fruit, number of seeds per fruit and test weight. The treatment, 1.2 µM Trichostatin A incubated for three days, showed a remarkable difference as traits such as total field emergence, seedling parameters and days to first flowering plant height, number of seeds per fruit and test weight deviated from the expected trend of decreasing growth parameters as the dose and incubation period increased. The study further revealed that the treatment (1.2 µM TSA incubated for three days) can be suggested for use in okra to induce epigenetic variations without significantly compromising the growth and vigour parameters of okra.