bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on English network teaching system Based on artificial intelligence and WBIETS system
Authors: Tan Minghua
Published: March 2020

The English teaching network system is a distant teaching based on Web. This teaching method can stimulate students' interests, so that students can acquire knowledge voluntarily, and automatic test paper generation is one of the most important modules in English teaching network system. This paper first describes the automatic test problems, is a constrained multi-objective problem; then the design of genetic algorithm to improve the test paper, put forward questions based encoding method and based on the difficulty and test points of F fitness function for dynamic adjustment of the parameters in the iterative process. Finally, it is verified by experiments that the test paper made by this method satisfies users' requests for questions, contents and scores, and at the same time, it also improves the running efficiency of random optimization algorithm by 7-17 times.
Simulated operation combines patient-specific guide technology is superior to conventional technology for supramalleolar osteotomy: a retrospective comparative study
Authors: Wang Chenggong , Xu Can , Li Yusheng , Li Mingqing , Zhong Da , Wang Long , Liu Hua
Published: March 2020

Background: Over the past seven years, our team has designed a simulated operation combines patient-specific guide (SO-PSI) assisted supramalleolar osteotomy (SMOT) method and applied it to the clinic. This study aimed to determine the difference between SO-PSI technology and conventional operation (CO) technology for SMOT in preoperative planning, intraoperative application and postoperative curative effect. Methods: We retrospectively analyzed the data of SMOT in our hospital between October 2014 and December 2018; 28 patients were enrolled and divided into CO group (n=17) and SO-PSI group (n=11); the mean follow-up time was 33.4 (range, 13 to 59) months. We analyzed and compared perioperative data, accuracy of preoperative planning and intraoperative application, difference between pre- and post-operative of radiologic ankle angles, and change of AOFAS score, VAS, range of motion of ankle and Takakura stage after operation. Then we performed statistical analyses. Results: All ankle alignments and positions of two groups have been recovered. Compared with CO group, SO-PSI group has a shorter operative time and a postoperative hospital stay, a decreased number of fluoroscopies and a reduction of albumin, a longer preoperative planning time and a preoperative hospital stay and an increased hospitalization expenses. In SO-PSI group, the ankle angles between preoperative planning and postoperative shown good correlation, but not in CO group; the mean tibial ankle center discrepancy of SO-PSI group was 1.86±1.06 mm. After follow-up, all radiologic parameters of two groups significantly improved, but the improvement of tibial anterior surface angle and tibiotalar tilt angle of SO-PSI group were more obvious than that of CO group; AOFAS score, VAS, range of motion of ankle and Takakura stage of two groups improved after operation; but overall the improvements of SO-PSI group were better than the CO group. Conclusions: SO-PSI technology can carry out the preoperative plan accurately and quickly in SMOT; In general, compared with the conventional technology, SO-PSI technology has advantages in preoperative planning, intraoperative application and postoperative curative effect. Keywords: Supramalleolar osteotomy; Simulated operation; Patient-specific guide; Accuracy of preoperative planning; Practicability of application
Quantitative evaluation of range and metabolic activity of hepatic alveolar echinococcosis lesion microenvironment using PET/CT and multi-site sampling method
Authors: Aini Abudusalamu , Yimingjiang Maiweilidan , Yasen Aimaiti , Ran Bo , Jiang Tiemin , Li Xiaohong , Wang Jian , Aihaiti Kasimu , Ainiwa Aisika , Abulizi Aubuduaini , Shao Yingmei , Aji Tuerganaili , Wen Hao
Published: March 2020

Background: Alveolar echinococcosis (AE) lesion microenvironment (LME) is crucial site where parasite-host interactions happen and of great significance during surgery and obtaining liver samples for basic research targeting immunology. However, little is known about quantification of LME range and its’ metabolic activity regarding different lesion types. Materials and Methods: A prospective analysis of LME from consecutive surgical AE cases with relevant imaging results was performed. Patients (n=39) received abdominal computed tomography (CT) and position emission tomography/computed tomography using 18F-fluodeoxyglucose (18F-FDG-PET/CT) within one week prior to surgery. Tumor to background ratios (TBRs) of standard uptake value (SUV) in PET/CT was calculated for corresponding LME regions. Multi-site sampling method (MSS, n=26) was introduced to obtain histological slides from LME at different levels off the lesion to evaluate immune cell infiltrative ranges quantitatively. At last, data was statistically analyzed from the perspective of different lesion types. Results: Altogether six major lesion categories have been identified based on different morphology and calcification pattern (A: non-calcified uniform density lesion; B: diffuse calcified solid lesion; C: half necrotic and half solid lesion with minor calcification; D: half necrotic and half solid lesion with obvious calcification; E: subtotal necrotic lesion with minor calcification; F: total necrotic lesion with obvious calcification). Statistical significances were resulted from TBRs calculation (A>B, A>D, A>F, BB+D+F, etc.). Less calcified lesions were evidenced with higher TBRs, however, not much was valuable for necrosis. The 95% CI of LME ranges were (10.0, 12.1) mm and (9.9, 14.0) mm by PET/CT and MSS. And, weak regressions between TBRs and LME ranges indicated by PET/CT or MSS (r2 respectively were 0.2436 and 0.3171) were observed. Conclusions: PET/CT showed distinct TBRs for different lesion types with heterogenic calcification. PET/CT and MSS had similar discoverability for LME ranges, which also varied among different lesion types. Higher activity meant wider LME range within certain limit. This pioneering study would be able to provide references for both surgical removal of lesions and sample acquisitions more accurately for basic research targeted to immunology.
Prevention of Surgical Site Infection by Gentamicin-Impregnated Collagen Sponge (Collashield® Double Action) in Patients Who Underwent Abdominal Surgery
Authors: Lee Jin Won , Chun Wan Joo , Kim Hae Sung
Published: March 2020

Background: The aim of this study was to evaluate the efficacy of the gentamicin-impregnated collagen sponge (GCS) in preventing SSI after abdominal surgeries. Methods: We conducted a retrospective study to evaluate the effect of GCS in preventing postoperative surgical site infection (SSI) in patients who underwent abdominal surgeries. 194 patients (111 male and 83 female; mean age, 62 years; range, 20-96 years) who underwent abdominal surgery between January 2017 and December 2018 in Chuncheon Sacred Heart Hospital were included in this study. The primary endpoint was the development of any SSI within 1 month postoperatively. Results: Of 194 patients studied, 30(20.1%, control group) and 6(13.3%, sponge group) in each group developed a SSI. However, subgroup analysis in patients with contaminated and dirty wound showed that SSI was occurred more frequently in the control group (15 of 36 [41.7%]) than in the sponge group (3 of 22 [13.6%]) (P = 0.04). Our data suggest that postoperative SSI can be reduced by using GCS in in the group of patients with contaminated and dirty wound classes. Conclusions: The SSI rate after abdominal surgeries was reduced in the group of patients with contaminated and dirty wound classes, by using GCS. We should consider to use GCS in patients with these type of wounds. Key words: Surgical Site Infection(SSI), Abdominal Surgery, Gentamicin-Impregnated
The Correlation of Hip Joint Passive Range of Motion with Lower Limbs Injuries in Professional Soccer Players
Authors: Karaminia Elham , Angoorani Hoomam , Mazaherinezhad Ali , Hassanmirzaei Bahar , Moghadam Navid
Published: March 2020

Introduction : Soccer is a popular sport with a high injury rate. Lower limb injuries are accounted for 85% of injuries in professional Soccer player. It is important to identify the predisposing factors in professional Soccer players for screening players at an increased risk of injury. It is unclear whether to assume limited hip range of motion (ROM) as one of these factors this study aimed to evaluate the association between hip passive ROM and lower limb injury in Iranian professional Soccer players.Design : This prospective cohort study was conducted on 266 male professional Soccer players (with a mean age of 24.91 ± 4.38 years) playing in the Iran Soccer Premium League during the 2017-2018 and 2018-2019 season. Baseline hip joint passive ROM was evaluated in all subjects. All athletes were followed for two consecutive seasons and their lower limb injuries causing at least an absent from a competitive match was reported. The association between hip ROM and the incidence of lower limb injuries was analyzed.Results : The most common lower limb injuries were ankle and foot injuries (12.4%), groin pain (11.5%) and muscle strain (10.9%), respectively. Restricted hip internal rotation (IR) was associated with a higher incidence of groin pain, ACL injury and knee injury. A significant correlation was observed between decreased hip external rotation (ER) with muscle strain and knee injuries. The higher knee injury was also associated with reduced hip abduction. There was no association between ankle and foot injuries and hip passive ROM.Conclusions : This study showed that hip passive ROM could be used to identify Soccer players at a higher risk of lower limb injuries. Hip rotational ROM and abduction limitation were significantly associated with the incidence of several common Soccer injuries, including groin pain, muscle strain and ACL/knee injuries and precedes their development and could be used as a screening tool for professional male soccer players.
Angiotensin II receptor 1 antibodies associate with post transplant focal segmental glomerulosclerosis and proteinuria
Authors: Abuzeineh Mohammad , Aala Amtul , Alasfar Sami , Alachkar Nada
Published: March 2020

Background

Angiotensin II type 1 receptors (AT1Rs) are expressed on podocytes, and play an essential role in the maintenance of vascular homeostasis. The presence of AT1R antibodies (AT1R-Abs) leads to activation of these receptors resulting in endothelial cell dysfunction and podocyte injury. We assessed the correlation between AT1R-Abs and the risk of post transplant FSGS.

Methods

This is a retrospective study included all kidney transplant recipients with positive AT1R-Abs (≥9 units/ml), who were transplanted and followed at our institution between 2006-2016. We assessed the development of biopsy proven FSGS and proteinuria by urine protein to creatinine ratio of ≥ 1 g/g.

Results

We identified 100 patients with positive AT1R-Ab at the time of kidney transplant biopsy or proteinuria. 49 (49%) recipients (FSGS group) had biopsy-proven FSGS and/or proteinuria. Pre-transplant hypertension was present in 89% of the FSGS group compared to 72% in the non-FSGS group, p =0.027. 43% of the FSGS group were on angiotensin converting enzyme inhibitors or angiotensin receptor blockers prior to transplant, compared to 25.5% in the non-FSGS group, p =0.06 . Primary idiopathic FSGS was the cause of ESRD in 20% of the FSGS group, compared to 6% in the comparison group; p =0.03. The allograft loss was significantly higher in the FSGS group 63% compared to 39% in non-FSGS. Odds ratio and 95% confidence interval were 2.66 (1.18 -5.99); p =0.017.

Conclusions

Our data suggests a potential association between AT1R-Abs and post transplant FSGS leading to worse allograft outcome. Therefore, AT1R-Abs may be considered a biomarker for FSGS post transplant.
Deep Factor Analysis for Weather Varied Sense-through-foliage Target Detection
Authors: Xue Wenling , Jiang Ting , Sun Xuebin , Zheng Xiaokun , Ding Xue
Published: March 2020

In this paper, the influence of seasonal variation on target detection accuracy and the effectiveness of deep factor analysis(DFA) in signal denoising are studied. To extensively verify the universality of the proposed DFA approach, a variety of target objects, including no target, human, wood board and iron cabinet targets, are measured in foliage environment under four different weather conditions. Then, after removing background noise from the collected data, deep factor analysis is carried out to further reduce the impact of noise. The experimental results show that the influence of weather variation on target detection can be effectively eliminated by the presented DFA, which can improve the average classification accuracy in all seasons. At the end of the paper, it is verified by cross validation that the DFA method can be stabilized at around 93% even in hazy day and snowy day which has stability and universality in any weather conditions, even in snowy and haze days.
Research on Personnel Performance Evaluation Model Based on Neural Wireless Network Data Mining Algorithm
Authors: Liang Wei , Li Tingyi
Published: March 2020

In order to effectively evaluate personnel performance, a distributed data mining algorithm for spatial networks based on BP neural wireless network is proposed. In the cloud computing environment, an excavator is used to construct multiple input multiple output spatial network data, analyze the data structure, and perform redundant data compression of massive data through time-frequency feature extraction. Combined with adaptive matching filtering method, the characteristics of the data are matched. The spatial frequency feature extraction method is used to locate the features of the multiple-input multiple-output spatial network data, and the fourth-order cumulant slice is used for reorganization. Data in time series. In order to improve the accuracy of data mining, the BP neural network is used to classify and identify the extracted data features to achieve the optimization of data mining. This algorithm improves the accuracy of personnel performance evaluation, and simultaneously establishes a hierarchical analysis and quantitative evaluation model for the performance of government managers, and adjusts the results of hierarchical statistical analysis on government administrators as needed. The performance evaluation and optimization of government administrators were introduced. The empirical analysis results show that the method has higher accuracy for government managers' performance evaluation, higher efficiency of big data processing and better integration.
Different distribution of maternal red cell antibodies and risk on the severe alloimmune haemolytic disease of the foetus in a Chinese Population: a retrospective cohort study on prenatal management
Authors: Li Si , He Zhiming , Luo Yanmin , Ji Yanli , Luo Guangping , Fang Qun , Gao Yu
Published: March 2020

BACKGROUND: The distribution of antibodies that cause haemolytic disease of the foetus (HDF) in China and the effects of multiple antibodies on the severity of HDF are needed to further evaluation. METHODS: A retrospective cohort study was conducted in two affiliated Hospitals of Sun Yat-sen University. Total 268 pregnant women and foetuses pairs were divided into four groups: anti-D, anti-D combined others, other single-antibody and other multiple-antibody. The obstetrics history, the incidence of severe HDF and foetal outcomes were collected and compared in each group. The logistics regression analysis and the survival analysis were conducted for analysis. RESULTS: Anti-D was the most common cause of HDF, followed by the anti-M of MNS system. No anti-K or isolate anti-c associated HDF was found. Upon the mothers with anti-D combined other antibodies, the incidence of severe HDF was increased than those with anti-D alone(P=0.046). Foetuses in the other single-antibody group had lower reticulocytes (P=0.007), more IUT times (P =0.007) and an earlier onset of severe HDF (P=0.003). High maternal antibody titer (P＜0.001), multiple affected pregnancies (P＜0.001) and other single-antibody (P=0.030) were the independent risk factor for HDF developing. And the lower foetal haematocrit (P =0.012) and higher reticulocyte counts (P =0.035) were the independent risk factors of severe HDF in the anaemia foetuses affected by RhD alloimmunization. CONCLUSION: The distribution of HDF-associated antibodies in China is different from that in western countries. Other single non-Rh(D) and multiple antibodies could both increase the risk of HDF but anti-D combined with other antibody would not influence the severity of the disease than anti-D alone.
Investigating the Effects of picture book on Reducing Anxiety Caused by Intravenous Catheterization among School-aged Children(Randomized Clinical Trial)
Authors: Kanikzadeh Masoumeh , Tahmasebi Rahim , bahreini farideh , jahanpour faezeh
Published: March 2020

Background: Injections are the main factors provoking pain, fear, and anxiety among hospitalized children. The purpose of this study is investigating the Effects of picture book on Reducing Anxiety Caused by Intravenous Catheterization among School-aged Children. Methods: In this randomized control trial study, seventy 6-12 year-old children were randomly assigned to either intervention or control group. Data was collected from 70 patients at Pediatric Emergency Room in the central Hospital in Bushehr- Iran during the summer and autumn, 2017. The subjects’ anxiety was evaluated before, during, and after the procedure by using Observation Scale of Behavior Distress instrument. Chi-square, Mann-Whitney and Wilcoxon tests were used for data analysis. Results: The mean anxiety scores of the intervention group was significantly less than those of control group after intervention. In addition, a significant difference was observed before and after the intervention in terms of anxiety levels. Conclusions: The school-aged children’s orientation on the intravenous catheterization and serum infusion play a significant role in controlling and reducing their anxiety during and after the intervention. Based on the result we suggested using the picture book to reduce the children anxiety before Intravenous Catheterization. Keywords: anxiety, pediatric, Intravenous Catheterization, education, picture book Trial registration: Current Controlled Trials: IRCT2017041712830N22). 2017-05-29
Evaluation of the TRIP13 level in breast cancer and insights into potential molecular pathways
Authors: Lan Jin , Huang Jingzhan , Gao Yuan , Sun Jingbo , Zhang Longshan , Deng Yunyao , Liu Lixin , Liu Xiaolong
Published: March 2020

Background: TRIP13 is a member belonging to a large AAA+ ATPases protein super family. Emerging evidences had shown that TRIP13 may serve as an oncogene However, the function of TRIP13 in BC has not yet been elucidated. Methods: By utilizing the multiple database across BC, we presented the expression of TRIP13 in BC tissue and normal control. We then verified the expression of TRIP13 in patients with BC by immunohistochemical (IHC) staining. Kaplan-Meier plots were used to perform the survival analysis. Further, gene ontology (GO) analysis, gene set enrichment analysis (GSEA), and PPI (protein-protein interaction) network were performed to explore the biological function and potential regular pathway of TRIP13 in BC. Results: The multiple database and immunohistochemical (IHC) showed that higher TRIP13 expression in BC tissue compared to normal tissue. TRIP13 was highly exprssed in lung metastasis lesion compared with primary tumor in our BALB/C mice 4T1 BC models. Kaplan-Meier plots also revealed that high TRIP13 expression correlated to poor survival in patients with BC. Moreover, GSEA analysis revealed that TRIP13 was primarily enriched in the processes of cell division and proliferation. Finally 10 hub genes with a high score of connectivity were filtered from the PPI network, including MAD2L1, CDC20, CDC5L, CDK1, CCNA2, BUB1B, RAD51, SPO11, KIF11 and AURKB. Conclusion: High TRIP13 expression predicted poor prognosis and contributed tumor growth and metastasis in the BC. Thus, ARL3 may be a prognostic marker and therapeutic target for glioma. TRIP13 may be a favorable biomarker and effective therapeutic target for BC. Keywords: TRIP13; breast cancer; metastasis; bioinformatic analysis, prognosis
Country and Gender Differences in the Association between Violence and Cigarette Smoking Among Youth
Authors: Abu-Rmeileh Niveen ME , Alderete Ethel , Husseini Abdullatif , Livaudais-Toman Jennifer , Perez-Stable Eliseo J
Published: March 2020

Background: Exposure to violence in youth may be associated with subsequent substance use and other adverse health effects. This study examined tow middle-income areas with different levels and types of exposure to violence. Methods: Association of exposure to verbal and physical violence with cigarette smoking in the West Bank oPt (2008) and in Jujuy Argentina (2006) was examined using cross-sectional surveys of 14 to 17-year old youth in 7th to 10th grade using probabilistic sampling. Results: Violence exposure rates were more than double for Palestinian girls (99.6% vs. 41.2%) and boys (98.7% vs. 41.1%) compared with Argentineans. Argentinean girls had current smoking rates eight times higher than Palestinian girls (56.2% vs. 7.1%). Exposure to verbal (OR 2.3; 95% CI=1.2-4.6) and physical (OR 2.0; 95% CI=1.1.-3.7) violence from family members increased the odds of smoking among Palestinian girls. Verbal violence from family (Adjusted OR1.6; 95% CI=1.2-2.1) and physical violence from nonfamily members (Adjusted OR 1.8; 95% CI=1.2-2.8) increased the odds of smoking among Argentinean boys. Conclusion: Findings highlight the importance of producing context and gender specific evidence, to inform and increase the impact of targeted prevention strategies.
The C-reactive Protein to Albumin Ratio Predicts Postoperative Complication in Patients who Undergo Gastrectomy for Gastric Cancer
Authors: Lee Jin Won , Chun Wan Joo , Kim Hae Sung
Published: March 2020

Background The aim of this study was to evaluate the preoperative C-reactive protein (CRP) to albumin ratio (CAR) of patients with gastric cancer and to investigate the factors correlated with perioperative complications. Methods From March 2016 to December 2019, 128 patients who underwent curative gastrectomy for gastric cancer were enrolled in a retrospective study. The preoperative cutoff value of the CAR for predicting postoperative complications was 0.265 on receiver operating characteristic (ROC) curve analysis. Clinical characteristics were compared between patients with complications (Clavien-Dindo grade ≥2, n = 20) and without complications (Clavien-Dindo grade <2, n = 108). Results On univariate and multivariate analyses, estimated blood loss (EBL) during the operation (HR 1.003, p = 0.039) and CAR (HR 2.832, p = 0.045) were independent predictors of postoperative complications. Conclusions A high CAR was significantly associated with postoperative complications in patients with gastric cancer.
Percutaneous left atrial appendage occlusion in nonvalvular atrial fibrillation patients with coronary heart disease
Authors: Zhang Juan , Jiang Xiaomin , Xie Dujiang , Luo Jie , Zhou Ling
Published: March 2020

Purpose To evaluate the 12-month outcomes of the percutaneous left atrial appendage occlusion (LAAO) procedure in nonvalvular atrial fibrillation (NVAF) patients with coronary heart disease (CAD). Materials and Methods 51 NVAF patients were consecutively accepted LAAO between June 2015 and July 2017. Patients were devided in two groups: 15 with CAD and 36 without CAD. All patients were followed up at 1st, 3rd, 6th, and 12th month after discharge with transesophageal echocardiography (TEE) examination repeated at the same time. Results Among these 51 patients (average age 64.4±10.0, 33.3% female), the procedural success rate was 98% (50/51). During 12-month follow-up, there were no hemorrhagic stroke or major bleeding. The risk of thromboembolism based on CHA2DS2-VASc score (3.1±1.6 vs. 5.2±1.1, P<0.001) and the risk of hemorrhage based on HAS-BLED score (2.2±1.2 vs. 3.1±1.0, P=0.009) in CAD group were significantly higher. The incidence of end-point events had no statistical difference between CAD and non-CAD. Compared with CAD patients who accepted long-term antithrombotic medication, there was no obvious difference in stroke rate and mortality in CAD accepted LAAO group, whereas a further reduction of hemorrhage (n=5/20% vs. n=0, P=0.039) was shown. A significant correlation (P<0.001, r=0.580) was detected between moderate or severe left atrial spontaneous echo contrast (LASEC) and the composite end point events. Conclusion There are similar safety and effectiveness for LAAO procedure in NVAF patients with CAD and without CAD under new oral anticoagulants applied post-implantation anticoagulation strategy. Meanwhile, LASEC is a predictive factor of LAAO in NVAF patients combined CAD.
DLBCL with amplification of JAK2/PD-L2 exhibits PMBCL-like CNA pattern and worse clinical outcome resembling those with MYD88 L265P mutation
Authors: Xue Xuemin , Huang Wenting , Qiu Tian , Guo Lei , Ying Jianming , Lv Ning
Published: March 2020

Background: Recently, copy number alteration (CNA) of 9p24.1 were demonstrated in 10% of diffuse large b-cell lymphoma (DLBCL), with gene expression and mutation profiles that were similar to those of primary mediastinal large B-cell lymphoma(PMBCL). However, their CNA-based profile and clinical impact still remain unclear. Methods: Multiplex ligation-dependent probe amplification were employed to investigate the prevalence of JAK2/PD-L2 amplification in DLBCL and their CNA-based pattern of driver genes. The clinical outcome and characteristics were also analyzed. Results: Using unsupervised hierarchical clustering, a small group of DLBCL (11.7%, 9/77) was clustered together with PMBCL as Cluster_2, demonstrating amplification of JAK2 (100%,9/9) and PD-L2 (77.8%,7/9). This subgroups of DLBCL demonstrated significant higher expression of PD-L1 than those with MYD88 L265P mutation(p=0.011). And they exhibited dismal OS and PFS as compared with DLBCL_others(p=0.021 and 0.015, respectively), which is similar to DLBCL with MYD88 L265P mutation. Conclusions: DLBCL with amplification of JAK2/PD-L2 exhibits CNA pattern that is similar to PMBCL, and worse clinical outcome that resembles those with MYD88 L265P mutation.
Alteration of gut microbiota in elderly postmenopausal women with osteoporosis
Authors: Li Yingjuan , Mu Guangjian , Ma Binbin , Lu Panpan , Huang Haiquan , Ren Liqun , Chen Hui , Rui Yunfeng , Zou Jihong , Liu Naifeng
Published: March 2020

Osteoporosis is a common bone disorder worldwide and causes bone fragility and fracture. Gut microbiota colonizes the gastrointestinal tract, and is associated with bone metabolism and osteoporosis. In our study, the alteration of gut microbiota in osteoporosis and its effects on bone metabolism were investigated. A total of 36 elderly postmenopausal osteoporotic women and 12 healthy controls were recruited, and their fecal samples were collected for 16S rRNA gene sequencing of gut microbiota on Illumina MiSeq platform. The venous blood and urine samples were also collected to determine the biochemical indexes. There was no obvious difference in Alpha diversity in the experiment group and control group, while differential Beta diversity was observed. The Partial Least Squares Discriminant Analysis (PLS-DA) model and variable importance in projection (VIP) scores showed that the osteoporotic women and healthy controls had different genera of Erysipelotrichaceae , Rothia , and Eubacterium . The metabolic function prediction of gut microbiota indicated that the experiment group had 634 unique functional categories, while the control group had 13 unique functional categories. The biochemical measurement revealed that the osteoclast activity indexes including urine N-terminal telopeptides of type I collagen (NTX), serum NTX, and serum C-terminal telopeptides of type I collagen (CTX) in the experiment group were higher than those in the control group, indicating that the osteoclast activity in osteoporotic women was increased. In addition, the correlation analysis of microbial metabolism with phenotypes showed the pathways in the significant modules of magenta, red, pink, and yellow were positively correlated with urine phosphate, urine creatinine, urine creatinine, serum calcium and other biochemical indexes. Collectively, our study identified the different genera between postmenopausal osteoporotic women and healthy controls, which might be potential targets for the treatment of osteoporosis.
Mining biomarkers for the occurrence, development and therapeutic response to infliximab of rheumatoid arthritis based on weighted gene co-expression network analysis
Authors: Wang Jian , Zhang Na , Chen Di , Fu Qiang , Wang Zaijun , Zhao Hui
Published: March 2020

Rheumatoid arthritis (RA) is a chronic systemic autoimmune and recurrent disease characterized by joint lesion with high disability rate and poor prognosis. Although anti-TNF therapy (infliximab) has been validated to be effective in controlling the symptoms of RA, more than one-third of patients cannot benefit from anti-TNF therapy. Therefore, it is critical to explore novel biomarkers associated with the occurrence and development of the disease, and therapeutic response of infliximab treatment. In this study, we first analyzed differentially expressed genes (DEGs) between RA patients and healthy controls. Subsequently, WGCNA was used to analyze the co-expression module (tan module) associated with the therapeutic response of infliximab treatment in RA, which further found that the genes (a total of 102) in the module tan were also the DEGs between RA patients and healthy controls (confirming that the close correlation of these genes with the occurrence and development of RA). Subsequently, enrichment analysis revealed that these 102 genes were mainly related to the body’s inflammatory immune response and the functions of NOD-like receptor signaling pathway as well as miR-146a-5p. Moreover, protein-protein interaction (PPI) was constructed on the 102 genes, followed by mining the key genes in the network (a total of 14 genes) by topological analysis. Finally, we confirmed that 10 genes (OAS1, STAT1, IFIT3, IFIT2, IFI6, RSAD2, OASL, GBP2, IRF7 and IFIT1) were potential biomarkers for the occurrence and development, as well as the therapeutic response against infliximab treatment in RA by analyzing the differences in the expressions of these genes between RA and healthy control samples, as well as drug response and non-response samples. Therefore, this study can provide guidance on medication selection and prognostic prediction of RA patients for clinicians, which can also identify relevant genes to provide therapeutic targets for precise medicine.
Identification of Stage-associated MicroRNAs in Lung Adenocarcinoma Based on Microarray Data
Authors: Yu Jiarui , Wang Wei , Gao Peng , Chen Siyuan , Liu Meiyue , Gu Jinling , Chen Xin , Sun Guogui
Published: March 2020

Background: Lung adenocarcinoma (LUAD) is the most aggressive and most frequently seen histological variant of lung cancers, comprising nearly 45% of the overall cases of lung carcinoma and its incidence has been increased significantly worldwide in the last several decades. However, there was limited available evidence with respect to the signalling pathways and miRNAs related to the LUAD.Methods: To identify the differentially-expressed miRNAs (DE-miRNAs) in different stages of LUAD, we performed a microarray on 514 LUAD and 54 normal tissues. At the mean time, the potentially targeted genes as well as the highly-enriched signaling pathways and the relevant protein–protein interaction (PPI) network were analyzed and constructed by using a series of bioinformatic methods. Moreover, the identified DE-miRNAs in different stages of the LUAD were verified by using the TCGA dataset.Results : Overall 41 down-regulated -and 82 up-regulated DE-miRNAs were identified, of which 1,716 potential targeted genes were selected. Moreover, the enriched pathways of the above genes were screened by using the GO term and KEGG pathway analyses, including the AMPK signalling pathway, FoxO signalling pathway, MAPK signalling pathway, PI3K-Akt signalling pathway and hippo signalling pathway.Conclusions: Our study could provide additional understanding of the underlying molecular events and increase the precision of prognostic prediction.
The relations between the level of Mu-opioid receptor (μORs) and postoperative analgesic use in patients undergoing septoplasty: A prospective randomized controlled trial.
Authors: GENCER MUZAFFER , Göçmen Ayşe Yeşim
Published: March 2020

Background In this study, the μ-Opioid receptor activity was examined before the septoplasty. We assessed preoperative μ-Opioid receptor activity association with postoperative pain level and second analgesic requirement in patients undergoing septoplasty. Methods In our prospective study, 120 adult patients underwent septoplasty from June 2015 to January 2019 were randomly divided into preop 2 groups. The first group (n=60) was patients given tramadol (1-2 mgr/kg) for postop analgesia, and the second group (control group) (n=60) was initially performed only fentanyl (1 µg/ kg-i.v.) in the induction. The μ-Opioid receptors activities were investigated in preoperative blood samples and compared post-op pain level and requiring time for second analgesic agent. The visual analogue score(VAS) was used to evaluate the postoperative pain degree (0 no pain,10 worst pain). Patients' post op VAS scores were evaluated upon arrival to recovery room, and at 1st, 3rd, 7th, 10th, and 24 th hours in postoperative period. Results Demographic data and perioperative variables was similar in both study group (p<0.05). There was no significant difference between the receptor levels in both two groups and the mean receptor level was 200.94 pg/ml. While the highest receptor level was 489.92 pg/ ml, the lowest receptor level was 94.56 pg/ml. In patients who used tradomal, as the levels of μ-Opioid receptors increased, VAS scores of patients and second analgesic use decreased in post-operative period. Vas scores were lower in the tramadol group compared to the control group. Patients in Tramadol group needed second pain killer later than patients in the Control group. Conclusions In this study, we revealed that postoperative pain level and second analgesic requirement decreased as μ-Opioid receptor levels increased in tramadol group.
Is Fear of Movement (FoM) in sports related activities a latent trait?: the item response model applied to the Photographic Series of Sports Activities for Anterior Cruciate Ligament Rupture (PHOSA-ACLR).
Authors: Lankveld Wim van , Pat-El Ron J. , Melick Nicky van , Cingel Robert van , Staal J. Bart
Published: March 2020

Background. Fear of Movement is an important factor predicting return to sports in Anterior Cruciate Ligament Reconstruction (ACLR). Fear for specific ACLR related movements can be addressed measuring the Photographic Sports Activities for ACLR (PHOSA-ACLR). This study was conducted to determine whether Fear of Movement assessed using the PHOSA-ACLR is a latent trait, and to analyse differences in PHOSA-ACLR in athletes with or without an ACL rupture. Methods. Data from three groups of participants were included in this study: 1) ACLR patients participated in a previous study measuring psychological dimensions after ACLR (n = 58); 2) first year physical therapy students at the HAN University of Applied Sciences (n = 169), and 3) junior football players that were enrolled in a training programme of the Professional Soccer club Vitesse (Arnhem) (n = 30). All participants completed the PHOSA-ACLR (scale score 0-10), and questions related to demographics. ACLR patients completed additional questionnaires to assess functioning and Fear of Harm. PHOSA-ACLR items were analysed with Item Response Theory using the Graded Response Model (GRM). Differences between three groups of participants were analysed using Univariate Analysis of Variance. Results. The two-parameter GRM with both location and discrimination (-2LL = -5485.05) showed a significantly better fit than the one-parameter model (-2LL = -5515.36), Common.EditSubmissionSteps.Transform.EquationText (1) = 30.31, p = 0.00). The items of the PHOSA-ACLR thus constitute a latent trait. The three groups of participants differed in reported fear of movement assessed with the PHOSA-ACLR after controlling for covariates gender and age. Physical therapy students reported higher levels of fear of movement compared to either ACLR patients and healthy soccer players (respectively 4.9 (95%CI 4.6-5.3); 3.6 (95%CI 2.5-3.7); 2.6 (95%CI 1.6-3.6)). Conclusion. PHOSA-ACLR items constitute a latent trait of fear of movement for ACLR specific movements. Contrary to expectations, PHOSA-ACLR is higher in first year physiotherapy students compared to patients rehabilitating from ACLR, and healthy junior soccer players. The PHOSA-ACLR might be useful for clinicians to identify high levels of fear of movement related to ACLR, and provide a tool to help the patient overcome fear of movement using exposure techniques.
Radiographic severity index in COVID-19 pneumonia: relationship to age and sex in 783 Italian patients
Authors: Borghesi Andrea , Zigliani Angelo , Masciullo Roberto , Golemi Salvatore , Maculotti Patrizia , Farina Davide , Maroldi Roberto
Published: March 2020

Purpose: To improve the risk stratification of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an experimental chest x-ray (CXR) scoring system for quantifying lung abnormalities was introduced in our Diagnostic Imaging Department. The purpose of this study was to retrospectively evaluate correlations between the CXR score and the age or sex of Italian patients infected with SARS-CoV-2. Material and Methods: Between March 4, 2020 and March 18, 2020, all CXR reports containing the new scoring system were retrieved. Only hospitalized patients with SARS-CoV-2 infection were enrolled. For each patient, age, sex, and the CXR report containing the highest score were considered for the analysis. Patients were also divided into seven groups according to age. Non-parametric statistical tests were used to examine the relationship between the severity of lung disease and the age or sex.Results: 783 Italian patients (532 males and 251 females) with SARS-CoV-2 infection were enrolled. The CXR score was significantly higher in males than in females only in groups aged 50 to 79 years. A significant correlation was observed between the CXR score and age in both males and females. Males aged 50 years or older and females aged 80 years or older showed a significantly increased risk of developing severe coronavirus disease 2019 (COVID-19).Conclusions: Males aged 50 years or older and females aged 80 years or older showed the highest risk of developing severe lung disease. Our results may help to identify the highest-risk patients and those who require specific treatment strategies.
Cytoreductive surgery (CRS) with hyperthermic intraoperative peritoneal chemotherapy (HIPEC) versus standard of care (SoC) in people with peritoneal metastases from colorectal, ovarian or gastric origin: protocol for a systematic review and individual participant data (IPD) meta-analyses of effectiveness and cost-effectiveness
Authors: Gurusamy Kurinchi Selvan , Vale Claire , Pizzo Elena , Bhanot R , Davidson Brian , Mould Tim , Mughal Muntzer , Saunders Mark , Aziz Omer , O’Dwyer Sarah
Published: March 2020

Background There is uncertainty about whether cytoreductive surgery (CRS) + hyperthermic intraoperative peritoneal chemotherapy (HIPEC) improves survival and/or quality of life compared to standard of care (SoC) in people with peritoneal metastases who can withstand major surgery. Primary objectives To compare the relative benefits and harms of CRS+HIPEC versus SoC in people with peritoneal metastases from colorectal, ovarian, or gastric cancers eligible to undergo CRS+HIPEC by a systematic review and individual participant data (IPD) meta-analysis. Secondary objectives To compare the cost-effectiveness of CRS+HIPEC versus SoC from a National Health Service (NHS) and personal social services (PSS) perspective using a model-based cost-utility analysis. Methods We will perform a systematic review of literature by updating the searches from MEDLINE, EMBASE, Cochrane library, Science Citation Index as well as trial registers. Two members of our team will independently screen the search results and identify randomised controlled trials (RCTs) comparing CRS+HIPEC versus SoC for inclusion based on full texts for articles shortlisted during screening. We will assess the risk of bias in the trials and obtain data related to baseline prognostic characteristics, details of how CRS+HIPEC and SoC were performed, how long people were followed-up, and outcome data related to overall survival, disease progression, health-related quality of life, treatment related complications, and resource utilisation data by contacting the study authors and obtaining data at the individual participant level. Using individual participant data (IPD), we will perform a two-step IPD, i.e. calculate the adjusted effect estimate from each included study and then perform a random-effects model meta-analysis. We will perform various subgroup analyses and metaregression to investigate potential sources of heterogeneity and identify whether a subset of participants benefits from CRS+HIPEC and perform sensitivity analyses to test the robustness of results to assumptions. We will also perform a model-based cost-utility analysis to assess whether CRS+HIPEC is cost-effective in the NHS setting.
Intercept of minute ventilation vs. carbon dioxide output relationship in chronic obstructive pulmonary disease: utility as an index of ventilatory inefficiency
Authors: Wang Haoyan , Lin Fang , Nie Shan , Zhao Ranran , Cao Min , Yuan Wei , Li Yunxiao , Tan Chunting , Xu Bo
Published: March 2020

Background: Ventilatory inefficiency is known to be a contributor to exercise intolerance in chronic obstructive pulmonary disease (COPD). The intercept of the minute ventilation (V̇E) vs. carbon dioxide output (V̇CO2) plot is a key ventilator inefficiency parameter. However, its relationships with lung hyperinflation (LH) and airflow limitation are not known. This study aimed to evaluate the correlations between the V̇E/V̇CO2 intercept and LH in COPD to determine its utility as an index of functional impairment.Methods: We conducted a retrospective analysis of data from 53 COPD patients and 14 healthy controls performed incremental cardiopulmonary exercise tests and resting pulmonary function. Ventilatory inefficiency was represented by parameters reflecting the V̇E/V̇CO2 nadir and slope (linear region), and intercept of the V̇E/V̇CO2 plot. Their correlations with measures of LH and airflow limitation were evaluated.Results: Compared to the control, the slope (30.58±3.62) and intercept (4.85±1.11) higher in COPDstages1-2, leading to a higher nadir (31.47±4.47) (p<0.05). Despite an even higher intercept in COPDstages3-4 (7.16±1.41), the slope diminished with disease progression (from 30.58±3.62 in COPDstages1-2 to 28.36±4.58 in COPDstages3-4). Compared to the V̇E/V̇CO2 nadir and V̇E/V̇CO2 slope, the intercept was better correlated with peak V̇E/maximal voluntary ventilation (MVV) (r=0.489, p<0.001) and peak V̇O2/watt (r=0.354, p=0.003). The intercept was also significantly correlated with RV/TLC (r=0.588, p<0.001), IC/TLC (r=-0.574, p<0.001), peak VT/TLC (r=-0.585, p<0.001); and airflow limitation forced expiratory volume in 1s (FEV1) % predicted (r=-0.606, p<0.001) and FEV1/forced vital capacity (FVC) (r=-0.629, p<0.001).Conclusion: V̇E/V̇CO2intercept was consistently better correlated with worsening static and dynamic lung hyperinflation and airflow limitation in COPD. V̇E/V̇CO2 intercept emerged as a useful index of ventilatory inefficiency across the severity spectrum of COPD patients.
Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at an STI Clinic in Guangzhou, China, 2016-2018.
Authors: Ke Wujian , Li Dongling , Tso Lai Sze , Wei Ran , Lan Yinyuan , Chen Zhengyu , Zhang Xiaohui , Wang Liuyuan , Liang Chunmei , Liao Yuying , Chen Huiru , Liu Yahui , Zheng Heping , Yang Ligang
Published: March 2020

BackgroundAntimicrobial resistance in M. genitalium is a growing clinical problem. We investigated the presence of mutations for macrolide and fluoroquinolone, two commonly used medical regiments for treatments in China. Our aim is to analyze the prevalence and diversity of mutations among M. genitalium-positive clinical specimens in Guangzhou, Guangdong, south China.MethodsA total of 154 stored M. genitalium positive specimens from men and women attending an STI clinic were tested for macrolide and fluoroquinolone mutations. M. genitalium was detected via TaqMan MGB real-time PCR with a sensitivity of five genome equivalents (geq)/reaction. Mutations associated with macrolide resistance were detected using primers targeting region V of the 23S rRNA gene. Fluoroquinolone resistant mutations were screened via primers targeting topoisomerase IV (parC) and DNA gyrase (gyrA).Results98.7% (152/154), 95.5% (147/154) and 90.3% (139/154) of M. genitalium positive samples produced sufficient amplicon for detecting resistance mutations in 23S rRNA, gyrA and parC genes, respectively. 66.4% (101/152), 0.7% (1/147) and 77.7% (108/139) samples manifested mutations in 23S rRNA, gyrA and parC genes, respectively. A2072G (59/101, 58.4%) and S83I (79/108, 73.1%) were highly predominating in 23S rRNA and parC genes, respectively. Two sample had amino acid alteration in gyrA (M95I and A96T, respectively). Two sample had two amino acid alterations in parC (S83I + D87Y). 48.6% (67/138) samples harbored both macrolide and fluoroquinolone resistance-associated mutations. The most common combination of mutations was A2072G (23S rRNA) and S83I (parC) (40/67, 59.7%). One sample had three amino acid changes in 23S rRNA, gyrA and parC genes (A2072G + A96T + S83I).ConclusionsThe high antimicrobial resistance rate of M. genitalium shows a worrisome trend in Guangzhou and suggests antimicrobial resistance testing and the development of new antibiotic regimens are crucial.
Comparison of Bailout and Planned Rotational Atherectomy for Severe Coronary Calcified Lesions
Authors: CAO Cheng-fu , MA Yu-liang , LI Qi , LIU Jian , ZHAO Hong , LU Ming-yu , wang weimin
Published: March 2020

Objective : To compare outcomes of bailout and planned rotational atherectomy (RA) in the treatment of severe calcified coronary lesions.
Methods: Data of patients treated with RA from 2017 to 2018 at a single-center registry were retrospectively analyzed. All patients were divided into planned RA and bailout RA groups, data between two groups were compared.
Results: A total of 190 patients were included in this study, 138 patients received planned RA and 52 patients received bailout RA. Baseline clinical characteristics had no significant differences between groups. The number of implanted stents and total stents length were similar. But the number of balloon (1.6±0.8 vs. 2.7±1.3, P <0.001), operation time (83.5±26.2 vs. 100.8±36.4min, P =0.007), fluoroscopy volume (941±482 vs. 1227±872mGy, P =0.012] and contrast amount （237±62 vs. 275±90ml, P =0.003）were all lower in planned RA group. Planned RA had a higher procedural success rate (99.3% vs. 92.3%, P =0.007) and a lower complication incidence (4.3% vs. 17.3%, P =0.009). But the cumulative 3-year incidences of MACE events (9.2% and 16.6%, log rank p 0.24) had no difference between groups.
Conclusion: For severe coronary artery calcification, although planned RA did not reduce MACE compared with bailout RA, but it can improve the immediate procedural success rate, reduce the incidence of complications, the operation time and the volume of contrast.
Keywords: severe coronary calcified lesions, planned rotational atherectomy, bailout rotational atherectomy