bluuebunny/crossref_metadata_2025 huggingface dataset:
The National After-School Athletics Program Participation as a Tool to Reduce the Risk of Obesity in Adolescents after One Year of Intervention: A Nationwide Study
Authors: Głąbska Dominika , Guzek Dominika , Mellová Blanka , Zadka Katarzyna , Żywczyk Katarzyna , Gutkowska Krystyna
Published: February 2019

Regular exercise during school hours is encouraged; however many children and adolescents fail to meet the recommendations during this time. Extracurricular activities may be a more appealing way for youth to achieve guidelines, and it is recommended that they attend two sessions each week. The aim of the study was to assess the influence of participation in a national physical activity program accompanied by nutritional education for trainers on the risk of obesity and body composition in a nationwide sample of boys and girls, after one year of intervention. The #goathletics Study was conducted in a group of 1014 adolescents aged 12–13: 507 individuals for the Athletics for All program (210 boys, 297 girls) and 507 pair-matched individuals not participating in any physical activity program (matching including: gender, age, city of residence). The body mass (kg), Body Mass Index (BMI) (kg/m2), waist circumference (WC) (cm), waist-to-height ratio (WHtR) (-) and body composition (%) (measured using bioelectrical impedance method) were compared in a gender-related sub-groups using t-Student test (for parametric distributions) or Mann-Whitney U test (for nonparametric distributions) and chi2 test (for the share of sub-groups). After one year of intervention, lower body mass percentile, BMI percentile, WC, WHtR and fat mass share, higher muscle mass share, as well as lower frequency of overweight/obesity and abdominal fat distribution were observed both for boys and girls participating in the physical activity intervention compared to the pair-matched controls. The after-school physical activity program accompanied by nutritional education for trainers may be a highly effective method for reducing the risk of obesity both for boys and girls, as regular participation is ensured.
Loneliness of Older Adults: Social Network and the Living Environment
Authors: Kemperman Astrid , van den Berg Pauline , Weijs-Perrée Minou , Uijtdewillegen Kevin
Published: February 2019

The social participation and integration of older adults are important aspects of healthy aging. However, in general, older adults have smaller social networks than their younger counterparts due to changes in their life cycle stage, such as retirement or age-related losses, along with a declining health and increasing mobility limitations. Consequently, with increasing age, an increasing proportion of older people experience feelings of loneliness and social isolation. Previous studies that have analyzed the relationships between loneliness, social networks, and the living environment have often been based on bivariate relationships or included only a limited number of variables. Therefore, the aim of this study was to analyze multiple relationships in a more comprehensive framework. Data were collected using a survey among 182 adults aged 65 years and over in the Netherlands. A Bayesian belief network (BBN) modeling approach was used that derives all direct and indirect relationships between the variables. The results showed that feelings of loneliness are directly related to satisfaction with one’s social network and neighborhood attachment and are indirectly related to perceived safety and satisfaction with local amenities and services. This knowledge is relevant to urban planners and policy makers who focus on creating livable and healthy social neighborhoods for the aging population.
Social Network Types and Health among Older Adults in Rural China: The Mediating Role of Social Support
Authors: Ye Liping , Zhang Xinping
Published: February 2019

This study aimed to identify social network types among older adults in rural China, to explore the relationship between social network types and the health of the older adults, and further, to examine the mediating role of social support in this relationship. A cross-sectional survey method was employed to investigate the health of adults aged 60 or older in rural areas of Hubei Province from 5 September 2018 to 15 October 2018; 405 samples were obtained. First, using k-means clustering analysis, we found five robust network types: diverse, restricted, family, friends and a specific type—family-restricted. Second, the results of multiple linear regression analysis showed that social network types were significantly associated with depressive symptoms and self-rated health in older adults. Older people with diverse friend networks were significantly associated with lower levels of depressive symptoms, whereas those with restricted and family-restricted networks were significantly associated with higher levels of depressive symptoms and lower levels of self-rated health. Finally, the results of multiple linear regression analysis confirmed that social support partially mediated the association of the identified social network types with depressive symptoms and self-rated health. Enriching the social network relationships of older adults and providing them with more social support should be conducive to promoting their mental and physical health.
Exploratory Determined Correlates of Physical Activity in Children and Adolescents: The MoMo Study
Authors: Schmidt Steffen CE , Schneider Jennifer , Reimers Anne Kerstin , Niessner Claudia , Woll Alexander
Published: February 2019

Background: Physical activity is an important contributor to reducing the risk for a variety of diseases. Understanding why people are physically active contributes to evidence-based planning of public health interventions because successful actions will target factors known to be related to physical activity (PA). Therefore the aim of this study is to identify the most meaningful correlates of PA in children and adolescents using a large, representative data set. Methods: Among n = 3539 (1801 boys) 6 to 17-year-old participants of the German representative Motorik-Modul baseline study (2003–2006) a total of 1154 different demographic, psychological, behavioral, biological, social and environmental factors were ranked according to their power of predicting PA using least absolute shrinkage and selection operator (LASSO) regressions. Results: A total of 18 (in girls) and 19 (in boys) important PA predictors from different, personal, social and environmental factors have been identified and ranked by LASSO. Peer modeling and physical self-concept were identified as the strongest correlates of PA in both boys and girls. Conclusions: The results confirm that PA interventions must target changes in different categories of PA correlates, but we suggest to focus particularly on the social environment and physical self-concept for interventions targeting children and adolescents in Germany nowadays. We also strongly recommend to repeatedly track correlates of PA, at least every 10 years, from representative samples in order to tailor contemporary PA interventions.
Relationship Between Internet Behaviors and Social Engagement in Middle-Aged and Older Adults in Taiwan
Authors: Chiu Ching-Ju
Published: February 2019

Aim: To examine older adults’ Internet use patterns and its relationship with social engagement. Methods: Telephonic interview data of older Internet users from two urban and two rural areas were analyzed (N = 248). Cluster analysis was used to identify their Internet use patterns. Multinomial logistic regression identified characteristics associated with the Internet usage groups, and the multiple regression was used to examine if the Internet usage pattern was associated with social engagement in real life. Results: The majority of older adults in Taiwan using the Internet were considered Leisure users (32%). Others were Sporadic (26%), Instrumental (21%), and Eager users (21%). Leisure and Eager users, but not Instrumental users, had significantly higher scores on social engagement compared with Sporadic users after controlling for sociodemographic and behavioral covariates. Eager Internet users were associated with 22.8% increase in the social engagement level, and Leisure users were associated with 31.2% increase in the social engagement level. Conclusions: Older adults with different Internet behaviors were associated with distinct sociodemographic and social engagement behaviors. Causal relationship is warranted for further investigation.
Cardiovascular Disease Healthcare Utilization in Sub-Saharan Africa: A Scoping Review
Authors: Chikafu Herbert , Chimbari Moses J.
Published: February 2019

Sub-Saharan African (SSA) countries face a growing burden of cardiovascular disease (CVD), attributed to economic, nutritional, demographic, and epidemiological transitions. These factors increase the prevalence of CVD risk factors, and the CVD burden overlaps with a high prevalence of infectious diseases. This review aimed to understand CVD healthcare utilization determinants and levels in SSA. We conducted a systematic search of the literature on major databases for the period 2008–2018 using exhaustive combinations of CVD and utilization indicators as search terms. Eighteen studies from eight countries were included in this review. Most studies (88.8%) followed the quantitative methodology and largely focused on inpatient stroke care. Two-thirds of patients sought care within 24 h of suffering a stroke, and the length of stay (LOS) in hospital ranged between 6 and 81 days. Results showed a rising trend of CVD admissions within total hospital admissions. Coverage of physiotherapy services was limited and varied between countries. While few studies included rural populations, utilization was found to be negatively associated with rural residence and socioeconomic status. There is a need to extend healthcare provision in SSA to ensure access to the CVD continuum of care.
Equity Impact Assessment of Interventions to Promote Physical Activity among Older Adults: A Logic Model Framework
Authors: Lehne Gesa , Voelcker-Rehage Claudia , Meyer Jochen , Bammann Karin , Gansefort Dirk , Brüchert Tanja , Bolte Gabriele
Published: February 2019

Reducing social inequalities in health and health determinants, including physical activity (PA), is a major challenge for public health. PA-promoting interventions are increasingly implemented. Little is known, however, about the impact of these interventions on social inequalities. For prioritizing interventions most likely to be effective in reducing inequalities, studies of PA interventions need to conduct equity impact assessments. The aim of this article is to describe the development of a logic model framework for equity impact assessments of interventions to promote PA. The framework was developed within the prevention research network AEQUIPA—Physical activity and health equity: primary prevention for healthy ageing, informed by an equity-focused systematic review, expert interviews, exploratory literature searches, and joint discussions within the network. The framework comprises a general equity-focused logic model to be adapted to specific interventions. The intervention-specific equity-focused logic models illustrate the key elements relevant for assessing social inequalities in study participation, compliance with and acceptance of interventions, as well as the efficacy of interventions. Future work within AEQUIPA will reveal which key elements are most critical for the interventions’ equity impacts. Equity impact assessments are beneficial for prioritizing interventions most likely to be effective in reducing health inequalities.
Evaluating Targeted Intervention on Coal Miners’ Unsafe Behavior
Authors: Tong Ruipeng , Zhang Yanwei , Yang Yunyun , Jia Qingli , Ma Xiaofei , Shao Guohua
Published: February 2019

Miners’ unsafe behavior is the main cause of roof accidents in coal mines, and behavior intervention plays a significant role in reducing the occurrence of miners’ unsafe behavior. However, traditional behavior intervention methods lack pertinence. In order to improve the intervention effect and reduce the occurrence of coal mine roof accidents more effectively, this study proposed a targeted intervention method for unsafe behavior. The process of targeted intervention node locating was constructed, and based on the analysis of 331 coal mine roof accidents in China, three kinds of targeted intervention nodes were located. The effectiveness of targeted intervention nodes was evaluated by using structural equation model (SEM) through randomly distributing questionnaires to miners of Pingdingshan coal. The results show that, in preventing roof accidents of coal mines, the targeted intervention nodes have a significant positive impact on the intervention effect. The method can also be applied to the safety management of other industries by adjusting the node location and evaluation process.
Education Policy for Migrant Children in Thailand and How It Really Happens; A Case Study of Ranong Province, Thailand
Authors: Tuangratananon Titiporn , Suphanchaimat Rapeepong , Julchoo Sataporn , Sinam Pigunkaew , Putthasri Weerasak
Published: February 2019

Health and education are interrelated, and it is for this reason that we studied the education of migrant children. The Thai Government has ratified ‘rights’ to education for all children in Thailand since 2005. However, there are gaps in knowledge concerning the implementation of education policy for migrants, such as whether and to what extent migrant children receive education services according to policy intentions. The objective of this study is to explore the implementation of education policy for migrants and the factors that determine education choices among them. A cross-sectional qualitative design was applied. The main data collection technique was in-depth interviews with 34 key informants. Thematic analysis with an intersectionality approach was used. Ranong province was selected as the main study site. Results found that Migrant Learning Centers (MLCs) were the preferable choice for most migrant children instead of Thai Public Schools (TPSs), even though MLCs were not recognized as formal education sites. The main reason for choosing MLCs was because MLCs provided a more culturally sensitive service. Teaching in MLCs was done in Myanmar’s language and the MLCs offer a better chance to pursue higher education in Myanmar if migrants migrate back to their homeland. However, MLCs still face budget and human resources inadequacies. School health promotion was underserviced in MLCs compared to TPSs. Dental service was underserviced in most MLCs and TPSs. Implicit discrimination against migrant children was noted. The Thai Government should view MLCs as allies in expanding education coverage to all children in the Thai territory. A participatory public policy process that engages all stakeholders, including education officials, health care providers, Non-Governmental Organizations (NGOs), MLCs’ representatives, and migrants themselves is needed to improve the education standards of MLCs, keeping their culturally-sensitive strengths.
Modeling of Disordered Protein Structures Using Monte Carlo Simulations and Knowledge-Based Statistical Force Fields
Authors: Ciemny Maciej Pawel , Badaczewska-Dawid Aleksandra Elzbieta , Pikuzinska Monika , Kolinski Andrzej , Kmiecik Sebastian
Published: February 2019

The description of protein disordered states is important for understanding protein folding mechanisms and their functions. In this short review, we briefly describe a simulation approach to modeling protein interactions, which involve disordered peptide partners or intrinsically disordered protein regions, and unfolded states of globular proteins. It is based on the CABS coarse-grained protein model that uses a Monte Carlo (MC) sampling scheme and a knowledge-based statistical force field. We review several case studies showing that description of protein disordered states resulting from CABS simulations is consistent with experimental data. The case studies comprise investigations of protein–peptide binding and protein folding processes. The CABS model has been recently made available as the simulation engine of multiscale modeling tools enabling studies of protein–peptide docking and protein flexibility. Those tools offer customization of the modeling process, driving the conformational search using distance restraints, reconstruction of selected models to all-atom resolution, and simulation of large protein systems in a reasonable computational time. Therefore, CABS can be combined in integrative modeling pipelines incorporating experimental data and other modeling tools of various resolution.
Cirsiliol Suppressed Epithelial to Mesenchymal Transition in B16F10 Malignant Melanoma Cells through Alteration of the PI3K/Akt/NF-κB Signaling Pathway
Authors: Prasad Priyanka , Vasas Andrea , Hohmann Judit , Bishayee Anupam , Sinha Dona
Published: February 2019

Malignant melanoma is a highly aggressive form of skin cancer which has a propensity for metastasis. Epithelial mesenchymal transition (EMT) plays a primordial role in the progression of metastatic disease. Metastatic melanoma is resistant to conventional therapies. Hence, researchers have been exploring alternative approaches, including the utility of bioactive phytochemicals to manage metastatic disease. In the present study, we investigated the potential of cirsiliol, a flavonoid isolated from Centaurea jacea L., in modulating the aggressive behavior of B16F10 metastatic melanoma cells, including EMT, and associated molecular mechanisms of action. Cirsiliol was found to be effective in restraining the colony formation and migration of fibronectin-induced B16F10 metastatic melanoma cells. Cirsiliol inhibited the activity and expression of matrix metalloproteinase-9 (MMP-9). Cirsiliol also suppressed the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (also known as Akt)/nuclear factor-κB (NF-κB) signaling pathway which, in turn, caused upregulation of E-cadherin and downregulation of N-cadherin, Snail and Twist. Based on these results, cirsiliol may be considered a promising compound against EMT in the therapeutic management of malignant melanoma.
Innovative Biomaterials for Bone Regrowth
Authors: Iaquinta Maria Rosa , Mazzoni Elisa , Manfrini Marco , D’Agostino Antonio , Trevisiol Lorenzo , Nocini Riccardo , Trombelli Leonardo , Barbanti-Brodano Giovanni , Martini Fernanda , Tognon Mauro
Published: February 2019

The regenerative medicine, a new discipline that merges biological sciences and the fundamental of engineering to develop biological substitutes, has greatly benefited from recent advances in the material engineering and the role of stem cells in tissue regeneration. Regenerative medicine strategies, involving the combination of biomaterials/scaffolds, cells, and bioactive agents, have been of great interest especially for the repair of damaged bone and bone regrowth. In the last few years, the life expectancy of our population has progressively increased. Aging has highlighted the need for intervention on human bone with biocompatible materials that show high performance for the regeneration of the bone, efficiently and in a short time. In this review, the different aspects of tissue engineering applied to bone engineering were taken into consideration. The first part of this review introduces the bone cellular biology/molecular genetics. Data on biomaterials, stem cells, and specific growth factors for the bone regrowth are reported in this review.
Transcriptome Analysis of Improved Wool Production in Skin-Specific Transgenic Sheep Overexpressing Ovine β-Catenin
Authors: Wang Jiankui , Cui Kai , Yang Zu , Li Tun , Hua Guoying , Han Deping , Yao Yanzhu , Chen Jianfei , Deng Xiaotian , Yang Xue , Deng Xuemei
Published: February 2019

β-Catenin is an evolutionarily conserved molecule in the canonical Wnt signaling pathway, which controls decisive steps in embryogenesis and functions as a crucial effector in the development of hair follicles. However, the molecular mechanisms underlying wool production have not been fully elucidated. In this study, we investigated the effects of ovine β-catenin on wool follicles of transgenic sheep produced by pronuclear microinjection with a skin-specific promoter of human keratin14 (k14). Both polymerase chain reaction and Southern blot analysis showed that the sheep carried the ovine β-catenin gene and that the β-catenin gene could be stably inherited. To study the molecular responses to high expression of β-catenin, high-throughput RNA-seq technology was employed using three transgenic sheep and their wild-type siblings. These findings suggest that β-catenin normally plays an important role in wool follicle development by activating the downstream genes of the Wnt pathway and enhancing the expression of keratin protein genes and keratin-associated protein genes.
Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks
Authors: Kuryk Lukasz , Møller Anne-Sophie W , Vuolanto Antti , Pesonen Sari , Garofalo Mariangela , Cerullo Vincenzo , Jaderberg Magnus
Published: February 2019

Oncolytic adenoviruses can trigger lysis of tumor cells, induce an antitumor immune response, bypass classical chemotherapeutic resistance strategies of tumors, and provide opportunities for combination strategies. A major challenge is the development of scalable production methods for viral seed stocks and sufficient quantities of clinical grade viruses. Because of promising clinical signals in a compassionate use program (Advanced Therapy Access Program) which supported further development, we chose the oncolytic adenovirus ONCOS-401 as a testbed for a new approach to scale up. We found that the best viral production conditions in both T-175 flasks and HYPERFlasks included A549 cells grown to 220,000 cells/cm2 (80% confluency), with ONCOS-401 infection at 30 multiplicity of infection (MOI), and an incubation period of 66 h. The Lysis A harvesting method with benzonase provided the highest viral yield from both T-175 and HYPERFlasks (10,887 ± 100 and 14,559 ± 802 infectious viral particles/cell, respectively). T-175 flasks and HYPERFlasks produced up to 2.1 × 109 ± 0.2 and 1.75 × 109 ± 0.08 infectious particles of ONCOS-401 per cm2 of surface area, respectively. Our findings suggest a suitable stepwise process that can be applied to optimizing the initial production of other oncolytic viruses.
Novel Zebrafish Mono-α2,8-sialyltransferase (ST8Sia VIII): An Evolutionary Perspective of α2,8-Sialylation
Authors: Chang Lan-Yi , Teppa Elin , Noel Maxence , Gilormini Pierre-André , Decloquement Mathieu , Lion Cédric , Biot Christophe , Mir Anne-Marie , Cogez Virginie , Delannoy Philippe , Khoo Kay Hooi , Petit Daniel , Guérardel Yann , Harduin-Lepers Anne
Published: February 2019

The mammalian mono-α2,8-sialyltransferase ST8Sia VI has been shown to catalyze the transfer of a unique sialic acid residues onto core 1 O-glycans leading to the formation of di-sialylated O-glycosylproteins and to a lesser extent to diSia motifs onto glycolipids like GD1a. Previous studies also reported the identification of an orthologue of the ST8SIA6 gene in the zebrafish genome. Trying to get insights into the biosynthesis and function of the oligo-sialylated glycoproteins during zebrafish development, we cloned and studied this fish α2,8-sialyltransferase homologue. In situ hybridization experiments demonstrate that expression of this gene is always detectable during zebrafish development both in the central nervous system and in non-neuronal tissues. Intriguingly, using biochemical approaches and the newly developed in vitro MicroPlate Sialyltransferase Assay (MPSA), we found that the zebrafish recombinant enzyme does not synthetize diSia motifs on glycoproteins or glycolipids as the human homologue does. Using comparative genomics and molecular phylogeny approaches, we show in this work that the human ST8Sia VI orthologue has disappeared in the ray-finned fish and that the homologue described in fish correspond to a new subfamily of α2,8-sialyltransferase named ST8Sia VIII that was not maintained in Chondrichtyes and Sarcopterygii.
The Implications of the Long Non-Coding RNA NEAT1 in Non-Cancerous Diseases
Authors: Prinz Felix , Kapeller Anita , Pichler Martin , Klec Christiane
Published: February 2019

Long non-coding RNAs (lncRNAs) are involved in a variety of biological and cellular processes as well as in physiologic and pathophysiologic events. This review summarizes recent literature about the role of the lncRNA nuclear enriched abundant transcript 1 (NEAT1) in non-cancerous diseases with a special focus on viral infections and neurodegenerative diseases. In contrast to its role as competing endogenous RNA (ceRNA) in carcinogenesis, NEAT1’s function in non-cancerous diseases predominantly focuses on paraspeckle-mediated effects on gene expression. This involves processes such as nuclear retention of mRNAs or sequestration of paraspeckle proteins from specific promoters, resulting in transcriptional induction or repression of genes involved in regulating the immune system or neurodegenerative processes. NEAT1 expression is aberrantly—mostly upregulated—in non-cancerous pathological conditions, indicating that it could serve as potential prognostic biomarker. Additional studies are needed to elucidate NEAT1’s capability to be a therapeutic target for non-cancerous diseases.
Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways
Authors: Mazibuko-Mbeje Sithandiwe E. , Dludla Phiwayinkosi V. , Roux Candice , Johnson Rabia , Ghoor Samira , Joubert Elizabeth , Louw Johan , Opoku Andy R. , Muller Christo J. F.
Published: February 2019

We previously demonstrated that an aspalathin-enriched green rooibos extract (GRE) reversed palmitate-induced insulin resistance in C2C12 skeletal muscle and 3T3-L1 fat cells by modulating key effectors of insulin signalling such as phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B (PI3K/AKT) and AMP-activated protein kinase (AMPK). However, the effect of GRE on hepatic insulin resistance is unknown. The effects of GRE on lipid-induced hepatic insulin resistance using palmitate-exposed C3A liver cells and obese insulin resistant (OBIR) rats were explored. GRE attenuated the palmitate-induced impairment of glucose and lipid metabolism in treated C3A cells and improved insulin sensitivity in OBIR rats. Mechanistically, GRE treatment significantly increased PI3K/AKT and AMPK phosphorylation while concurrently enhancing glucose transporter 2 expression. These findings were further supported by marked stimulation of genes involved in glucose metabolism, such as insulin receptor (Insr) and insulin receptor substrate 1 and 2 (Irs1 and Irs2), as well as those involved in lipid metabolism, including Forkhead box protein O1 (FOXO1) and carnitine palmitoyl transferase 1 (CPT1) following GRE treatment. GRE showed a strong potential to ameliorate hepatic insulin resistance by improving insulin sensitivity through the regulation of PI3K/AKT, FOXO1 and AMPK-mediated pathways.
The Biochemical and Pharmacological Properties of Ozone: The Smell of Protection in Acute and Chronic Diseases
Authors: Di Mauro Rosaria , Cantarella Giuseppina , Bernardini Renato , Di Rosa Michelino , Barbagallo Ignazio , Distefano Alfio , Longhitano Lucia , Vicario Nunzio , Nicolosi Daniela , Lazzarino Giacomo , Tibullo Daniele , Gulino Maria Eugenia , Spampinato Mariarita , Avola Roberto , Li Volti Giovanni
Published: February 2019

Ozone therapy has been widely used in everyday clinical practice over the last few years, leading to significant clinical results in the treatment of herniated discs and pain management. Nevertheless, further studies have demonstrated its potential efficacy and safety under other clinical and experimental conditions. However, some of these studies showed controversial results regarding the safety and efficacy of ozone therapy, thus mining its potential use in an everyday clinical practice. To this regard, it should be considered that extensive literature review reported the use of ozone in a significant different dose range and with different delivery systems. The aim of the present review is to describe the various pharmacological effects of ozone in different organs and clinical conditions and to provide possible biochemical and molecular insights for ozone biological properties, thus providing a possible explanation for various controversial clinical outcomes described in the scientific literature.
Analysis of the miRNA Expression Profiles in the Zearalenone-Exposed TM3 Leydig Cell Line
Authors: Wang Mingyang , Wu Weiwei , Li Lin , He Jianbin , Huang Sheng , Chen Si , Chen Jia , Long Miao , Yang Shuhua , Li Peng
Published: February 2019

Zearalenone (ZEN), an important environmental pollutant, can cause serious harm to human and animal health. The aim of our study was to examine the effect of zearalenone (ZEN) on miRNA expression profiles in the mouse Leydig cell line (TM3 Leydig cell line) by miRNA sequencing. The effect of ZEN on the viability of TM3 Leydig cells was verified by Cell Counting Kit-8 (CCK-8). MiRNA sequencing was performed 24 h after the exposure of TM3 Leydig cells with 50 μmol/L of ZEN. Bioinformatics predicted the miRNA target genes, performed Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, and conducted miRNA-gene-pathway mapping to show the relationship between miRNA, the target gene, and the signalling pathway. The expression levels of miRNA and the miRNA target genes associated with ZEN toxicology were verified by quantitative real-time polymerase chain reaction. The miRNA sequencing revealed a significant change (p < 0.05) in the 197 miRNAs in the ZEN-treated and control groups, among which 86 were up-regulated and 111 were down-regulated. GO analysis of the target genes of these miRNAs indicated various biological functions. KEGG analysis showed that the predicted miRNA target genes were involved in signalling pathways, such as cancer, apoptosis, and oxidation, namely, the Ras signalling pathway, Rap1 signalling pathway, PI3K-AKT signalling pathway, Foxo signalling pathway, and AMPK signalling pathway. These results suggest that ZEN, as an estrogen-like toxin, is regulated by microRNAs. Our results can help to examine the toxicological effects of ZEN-regulated miRNAs on germ cells.
Niche Differentiation between Two Sympatric Cubitermes Species (Isoptera, Termitidae, Cubitermitinae) Revealed by Stable C and N Isotopes
Authors: Josens Guy , Makatia Wango Solange Patricia
Published: February 2019

Many African termite species are true soil-feeders: how can they coexist, sometimes with high densities? How do they separate their trophic niches? Preliminary results suggest that two coexisting Cubitermes species forage in different soil layers, and stable C and N isotopes show that they feed on different organic material. Cubitermes aff. ugandensis forages near the soil surface whereas C. aff. sankurensis forages in deeper layers; however, unexpectedly, the former shows a higher δ15N than the latter, highlighting, for the first time, a trophic niche differentiation between two sympatric true soil feeders bearing different enteric valve patterns.
Landscape and Environmental Factors Influencing Stage Persistence and Abundance of the Bamboo Mosquito, Tripteroides bambusa (Diptera: Culicidae), across an Altitudinal Gradient
Authors: Chaves Luis Fernando , Friberg Mariel D. , Jian Jiun-Yu , Moji Kazuhiko
Published: February 2019

The bamboo mosquito, Tripteroides bambusa (Yamada) (Diptera: Culicidae), is a common insect across East Asia. Several studies have looked at the ecology of Tr. bambusa developmental stages separately, but little is known about the factors associated with the persistence (how often) and abundance (how many individuals) of Tr. bambusa stages simultaneously studied across a heterogeneous landscape. Here, we ask what environmental and landscape factors are associated with the persistence and abundance of Tr. bambusa stages across the altitudinal gradient of Mt. Konpira, Nagasaki City, Japan. During a season-long study we counted 8065 (7297 4th instar larvae, 670 pupae and 98 adults) Tr. bambusa mosquitoes. We found that persistence and abundance patterns were not associated among stages, with the exception of large (4th instar) and small (1st to 3rd instars) larvae persistence, which were positively correlated. We also found that relative humidity was associated with the persistence of Tr. bambusa aquatic stages, being positively associated with large and small larvae, but negatively with pupae. Similarly, landscape aspect changed from positive to negative the sign of its association with Tr. bambusa pupae and adults, highlighting that environmental associations change with life stage. Meanwhile, Tr. bambusa abundance patterns were negatively impacted by more variable microenvironments, as measured by the negative impacts of kurtosis and standard deviation (SD) of environmental variables, indicating Tr. bambusa thrives in stable environments, suggesting this mosquito species has a finely grained response to environmental changes.
Growth and Debt: An Endogenous Smooth Coefficient Approach
Authors: Koroglu Mustafa
Published: February 2019

The new growth theories with an emphasis on fundamental determinants such as institutions suggest a non-linear cross-country growth process. In this paper, we investigate the public debt and economic growth relationship using the semi-parametric smooth coefficient approach that allows democracy to influence this relationship and parameter heterogeneity in the unknown functional form and addresses the endogeneity of variables. We find results consistent with the previous literature that identified a significant adverse effect of public debt on growth for the countries below a particular democracy level. However, we also find conclusive evidence that countries with high institutional quality have an adverse effect of public debt on growth for the period 1980–2009, as well as for the extended period including the years 2010–2014. A 10-percentage point increase in the debt-to-GDP ratio is associated with a 0.12% and 0.07% decrease in the subsequent 10-year period real GDP growth rate for the zero democracy countries and for the countries with a democracy score of 10, respectively.
Single-Composition White Light Emission from Dy3+ Doped Sr2CaWO6
Authors: Dai Yannan , Yang Shuai , Shan Yongkui , Duan Chun-Gang , Peng Hui , Yang Fan , Zhao Qingbiao
Published: February 2019

A series of Dy3+ ion doped Sr2CaWO6 phosphors with double perovskite structure were synthesized by traditional high temperature solid-state method. It was found that there is significant energy transfer between Dy3+ and the host lattice, and the intensities of emission peaks at 449 nm (blue), 499 nm (cyan), 599 nm (orange), 670 nm (red), and 766 nm (infra-red) can be changed by adjusting the concentration of dopant amount of Dy3+ ion in Sr2CaWO6. The correlated color temperature of Dy3+ ion doped Sr2CaWO6 phosphors can be tuned by adjusting the concentration of Dy3+ ion. Upon optimal doping at 1.00 mol% Dy3+, white light with chromaticity coordinate (0.34, 0.33) was emitted under excitation at 310 nm. Thus, single composition white emission is realized in Dy3+ doped Sr2CaWO6.
Solid State Phase Equilibria of an Al–Sn–Y Ternary System
Authors: Yang Wenchao , Liu Moumiao , Feng Junli , Wu Jingwu , Mao Jun , Du Zaixiang , Ke Xiaojun , Zhang Xinjiang , Zhan Yongzhong
Published: February 2019

A complete understanding of the solid-state phase equilibria of the ternary Al–Sn–Y system is essential for the development of both Al-based structural materials and Sn-based lead-free solders. In this work, the phase relationships in the Al–Sn–Y ternary system at 473 K were investigated mainly by means of X-ray powder diffraction (XRD), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) with energy disperse spectroscopy (EDS) analysis. The existence of 12 binary compounds, namely Sn3Y, Sn5Y2, Sn2Y, Sn10Y11, Sn4Y5, Sn3Y5, AlY2, Al3Y5, Al2Y3, AlY, Al2Y and α–Al3Y, was confirmed. Controversial phases (Sn5Y2 and Al3Y5) were found in this work. This isothermal section consisted of 15 single-phase regions, 27 two-phase regions and 13 three-phase regions. No ternary compounds were found and none of the other phases in this system revealed a remarkable solid solution at 473 K.
Mannich Base as Corrosion Inhibitors for N80 Steel in a CO2 Saturated Solution Containing 3 wt % NaCl
Authors: Tang Mingjin , Li Jianbo , Li Zhida , Fu Luoping , Zeng Bo , Lv Jie
Published: February 2019

In this paper, a corrosion inhibitor containing nitrogen atoms and a conjugated π bond was synthesised, and its final product synthesised by the optimal conditions of the orthogonal test results is named multi-mannich base (MBT). The corrosion inhibition effect on the N80 steel sheet of the corrosion inhibitor was evaluated in a CO2 saturated solution containing 3 wt % NaCl; the corrosion rate was 0.0446 mm/a and the corrosion inhibition rate was 90.4%. Through electrochemical and adsorption theory study, MBT is a mixed corrosion inhibitor that mainly shows cathode suppression capacity. The adsorption of MBT on the surface of the steel sheet follows the Langmuir adsorption isotherm; it can be spontaneously adsorbed on the surface of the N80 steel sheet, which has a good corrosion inhibition effect. The surface of the N80 steel sheet was microscopically characterised by atomic force microscope (AFM). It can be seen from the results that the N80 steel sheet with MBT added is significantly different from the blank control group; the surface of the steel sheet is relatively smooth, indicating that MBT forms an effective protective film on the surface of N80 steel, which inhibits the steel sheet.
Synthesis, Characterization, and Modification of Alumina Nanoparticles for Cationic Dye Removal
Authors: Chu Thi Phuong Minh , Nguyen Ngoc Trung , Vu Thi Lan , Dao Thi Huong , Dinh Lan Chi , Nguyen Hai Long , Hoang Thu Ha , Le Thanh Son , Pham Tien Duc
Published: February 2019

In the present study, alumina nanoparticles (nano-alumina) which were successfully fabricated by solvothermal method, were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), and Brunauer–Emmett–Teller (BET) methods. The removal of cationic dye, Rhodamine B (RhB), through adsorption method using synthesized nano-alumina with surface modification by anionic surfactant was also investigated. An anionic surfactant, sodium dodecyl sulfate (SDS) was used to modify nano-alumina surface at low pH and high ionic strength increased the removal efficiency of RhB significantly. The optimum adsorption conditions of contact time, pH, and adsorbent dosage for RhB removal using SDS modified nano-alumina (SMNA) were found to be 120 min, pH 4, and 5 mg/mL respectively. The RhB removal using SMNA reached a very high removal efficiency of 100%. After four times regeneration of adsorbent, the removal efficiency of RhB using SMNA was still higher than 86%. Adsorption isotherms of RhB onto SMNA at different salt concentrations were fitted well by a two-step model. A very high adsorption capacity of RhB onto SMNA of 165 mg/g was achieved. Adsorption mechanisms of RhB onto SMNA were discussed on the basis of the changes in surface modifications, the change in surface charges and adsorption isotherms.
Study of Two Bovine Bone Blocks (Sintered and Non-Sintered) Used for Bone Grafts: Physico-Chemical Characterization and In Vitro Bioactivity and Cellular Analysis
Authors: Gehrke Sergio Alexandre , Mazón Patricia , Pérez-Díaz Leticia , Calvo-Guirado José Luis , Velásquez Pablo , Aragoneses Juan Manuel , Fernández-Domínguez Manuel , De Aza Piedad N.
Published: February 2019

In this work, the physicochemical properties and in vitro bioactivity and cellular viability of two commercially available bovine bone blocks (allografts materials) with different fabrication processes (sintered and not) used for bone reconstruction were evaluated in order to study the effect of the microstructure in the in vitro behavior. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectrometry, mechanical resistance of blocks, mercury porosimetry analysis, in vitro bioactivity, and cell viability and proliferation were performed to compare the characteristics of both allograft materials against a synthetic calcium phosphate block used as a negative control. The herein presented results revealed a very dense structure of the low-porosity bovine bone blocks, which conferred the materials’ high resistance. Moreover, relatively low gas, fluid intrusion, and cell adhesion were observed in both the tested materials. The structural characteristics and physicochemical properties of both ceramic blocks (sintered and not) were similar. Finally, the bioactivity, biodegradability, and also the viability and proliferation of the cells was directly related to the physicochemical properties of the scaffolds.
Effect of Temperature and Water Absorption on Low-Velocity Impact Damage of Composites with Multi-Layer Structured Flax Fiber
Authors: Shen Yiou , Zhong Junjie , Cai Shenming , Ma Hao , Qu Zehua , Guo Yichun , Li Yan
Published: February 2019

Temperature and moisture can cause degradation to the impact properties of plant fiber-based composites owing to their complex chemical composition and multi-layer microstructure. This study focused on experimental characterization of the effect of important influencing factors, including manufacturing process temperature, exposure temperature, and water absorption, on the impact damage threshold and damage mechanisms of flax fiber reinforced composites. Firstly, serious reduction on the impact damage threshold and damage resistance was observed, this indicated excessive temperature can cause chemical decomposition and structural damage to flax fiber. It was also shown that a moderate high temperature resulted in lower impact damage threshold. Moreover, a small amount of water absorption could slightly improve the damage threshold load and the damage resistance. However, more water uptake caused severe degradation on the composite interface and structural damage of flax fiber, which reduced the impact performance of flax fiber reinforced composites.
Dynamic Crushing Analysis of a Three-Dimensional Re-Entrant Auxetic Cellular Structure
Authors: Wang Tao , Li Zhen , Wang Liangmo , Ma Zhengdong , Hulbert Gregory M.
Published: February 2019

Dynamic behaviors of the three-dimensional re-entrant auxetic cellular structure have been investigated by performing beam-based crushing simulation. Detailed deformation process subjected to various crushing velocities has been described, where three specific crushing modes have been identified with respect to the crushing velocity and the relative density. The crushing strength of the 3D re-entrant auxetic structure reveals to increase with increasing crushing velocity and relative density. Moreover, an analytical formula of dynamic plateau stress has been deduced, which has been validated to present theoretical predictions agreeing well with simulation results. By establishing an analytical model, the role of relative density on the energy absorption capacity of the 3D re-entrant auxetic structure has been further studied. The results indicate that the specific plastic energy dissipation is increased by increasing the relative density, while the normalized plastic energy dissipation has an opposite sensitivity to the relative density when the crushing velocity exceeds the critical transition velocity. The study in this work can provide insights into the dynamic property of the 3D re-entrant auxetic structure and provides an extensive reference for the crushing resistance design of the auxetic structure.
Dual BACE1 and Cholinesterase Inhibitory Effects of Phlorotannins from Ecklonia cava—An In Vitro and in Silico Study
Authors: Lee Jinhyuk , Jun Mira
Published: February 2019

Alzheimer′s disease (AD) is one of the most common neurodegenerative diseases with a multifactorial nature. β-Secretase (BACE1) and acetylcholinesterase (AChE), which are required for the production of neurotoxic β-amyloid (Aβ) and the promotion of Aβ fibril formation, respectively, are considered as prime therapeutic targets for AD. In our efforts towards the development of potent multi-target, directed agents for AD treatment, major phlorotannins such as eckol, dieckol, and 8,8′-bieckol from Ecklonia cava (E. cava) were evaluated. Based on the in vitro study, all tested compounds showed potent inhibitory effects on BACE1 and AChE. In particular, 8,8′-bieckol demonstrated the best inhibitory effect against BACE1 and AChE, with IC50 values of 1.62 ± 0.14 and 4.59 ± 0.32 µM, respectively. Overall, kinetic studies demonstrated that all the tested compounds acted as dual BACE1 and AChE inhibitors in a non-competitive or competitive fashion, respectively. In silico docking analysis exhibited that the lowest binding energies of all compounds were negative, and specifically different residues of each target enzyme interacted with hydroxyl groups of phlorotannins. The present study suggested that major phlorotannins derived from E. cava possess significant potential as drug candidates for therapeutic agents against AD.
Neurymenolide A, a Novel Mitotic Spindle Poison from the New Caledonian Rhodophyta Phacelocarpus neurymenioides
Authors: Motuhi Sofia-Eléna , Feizbakhsh Omid , Foll-Josselin Béatrice , Baratte Blandine , Delehouzé Claire , Cousseau Arnaud , Fant Xavier , Bulinski Jeannette Chloë , Payri Claude Elisabeth , Ruchaud Sandrine , Mehiri Mohamed , Bach Stéphane
Published: February 2019

The marine α-pyrone macrolide neurymenolide A was previously isolated from the Fijian red macroalga, Neurymenia fraxinifolia, and characterized as an antibacterial agent against antibiotic-resistant strains that also exhibited moderate cytotoxicity in vitro against cancer cell lines. This compound was also shown to exhibit allelopathic effects on Scleractinian corals. However, to date no mechanism of action has been described in the literature. The present study showed, for the first time, the isolation of neurymenolide A from the New Caledonian Rhodophyta, Phacelocarpus neurymenioides. We confirmed the compound’s moderate cytotoxicity in vitro against several human cell lines, including solid and hematological malignancies. Furthermore, we combined fluorescence microscopy and flow cytometry to demonstrate that treatment of U-2 OS osteosarcoma human cells with neurymenolide A could block cell division in prometaphase by inhibiting the correct formation of the mitotic spindle, which induced a mitotic catastrophe that led to necrosis and apoptosis. Absolute configuration of the stereogenic center C-17 of neurymenolide A was deduced by comparison of the experimental and theoretical circular dichroism spectra. Since the total synthesis of this compound has already been described, our findings open new avenues in cancer treatment for this class of marine molecules, including a new source for the natural product.