bluuebunny/crossref_metadata_2025 huggingface dataset:
3D Evolutionary Reconstruction of Scalar Fields in the Gas-Phase
Authors: Unterberger Andreas , Kempf Andreas , Mohri Khadijeh
Published: May 2019

An evolutionary reconstruction technique (ERT) was developed for three-dimensional (3D) reconstruction of luminescent objects, in particular turbulent flames for the first time. The computed tomography (CT) algorithm is comprised of a genetic algorithm (GA) and a ray-tracing software. To guide the reconstruction process, a mask is introduced. It uses a Metropolis algorithm (MA) to sample locations where specific genetic operators can be applied. Based on an extensive parameter study, performed on several types of phantoms, the ability of our algorithm for 3D reconstructions of fields with varying complexities is demonstrated. Furthermore, it was applied to three experiments, to reconstruct the instantaneous chemiluminescence field of a bunsen flame, a highly turbulent swirl flame and the turbulent Cambridge-Sandia stratified flame. Additionally, we show direct and quantitative comparison to an advanced computed tomography of chemiluminescence (CTC) method that is based on an algebraic reconstruction technique (ART). The results showed good agreement between CTC and ERT using both phantom data from flame simulations, and experimental data.
Framework for Assessment of Eco-Safe Rural Roads in Panchase Geographic Region in Central–Western Nepal Hills
Authors: Devkota Sanjaya , Shakya Narendra Man , Sudmeier-Rieux Karen
Published: May 2019

Rural roads are important for the communities in the hilly areas of Nepal as they introduce livelihood opportunities at the local level, and provide better access to the healthcare, education, and resources. Yet, most of the rural roads in Nepal are unplanned and non-engineered, and these roads are often closed for many months during and after the monsoon. Such roads require huge investments, especially post-monsoon, to clear debris and to keep them operational. In parallel, there is evidence that such roads lead to a large number of slope failures and accelerated sedimentation, which degrade the environment and ecosystem services. To remedy such roadside slope failures, eco-engineering practices were tested and demonstrated in partnership with three communities in the Panchase Region of the Nepal’s Central–Western hills. Eco-engineering is a hybrid approach, combining civil engineering works for drainage and slope stability, with the plantation of deep-rooted vegetation. It is one activity contributing to nature-based solutions (NbS) for the sustainable and long-term operation of the rural roads in the Panchase geographic region. This paper describes the inter-disciplinary and community-based research, monitoring, and evaluation methods applied, including the establishment of onsite demonstration plots and rhizotrons in which key performance indicator (KPI) analysis of plant species was performed. The results demonstrated the effectiveness of eco-engineering for reducing risk, while creating ecological co-benefits along rural roads (or eco-safe roads) in hilly areas. Based on this research, an “eco-safe rural road assessment framework” was developed, outlining the systematic process to be followed for the design of eco-safe rural roads for more sustainable road construction and maintenance. The eco-engineering practices which are being promoted by this framework were accepted by communities and could be further implemented by local government bodies and upscaled in other similar hilly areas around the country.
Vehicle Speed Estimation Based on 3D ConvNets and Non-Local Blocks
Authors: Dong Huanan , Wen Ming , Yang Zhouwang
Published: May 2019

Vehicle speed estimation is an important problem in traffic surveillance. Many existing approaches to this problem are based on camera calibration. Two shortcomings exist for camera calibration-based methods. First, camera calibration methods are sensitive to the environment, which means the accuracy of the results are compromised in some situations where the environmental condition is not satisfied. Furthermore, camera calibration-based methods rely on vehicle trajectories acquired by a two-stage tracking and detection process. In an effort to overcome these shortcomings, we propose an alternate end-to-end method based on 3-dimensional convolutional networks (3D ConvNets). The proposed method bases average vehicle speed estimation on information from video footage. Our methods are characterized by the following three features. First, we use non-local blocks in our model to better capture spatial–temporal long-range dependency. Second, we use optical flow as an input in the model. Optical flow includes the information on the speed and direction of pixel motion in an image. Third, we construct a multi-scale convolutional network. This network extracts information on various characteristics of vehicles in motion. The proposed method showcases promising experimental results on commonly used dataset with mean absolute error (MAE) as 2.71 km/h and mean square error (MSE) as 14.62 .
Effects of the Addition of Flaxseed and Amaranth on the Physicochemical and Functional Properties of Instant-Extruded Products
Authors: Tobias-Espinoza Jazmin L. , Amaya-Guerra Carlos A. , Quintero-Ramos Armando , Pérez-Carrillo Esther , Núñez-González María A. , Martínez-Bustos Fernando , Meléndez-Pizarro Carmen O. , Báez-González Juan G. , Ortega-Gutiérrez Juan A.
Published: May 2019

The addition of flaxseed and amaranth on the physicochemical, functional, and microstructural changes of instant-extruded products was evaluated. Six mixtures with different proportions of amaranth (18.7–33.1%), flaxseed (6.6–9.3%), maize grits (55.6–67.3%) and minor ingredients (4.7%) were extruded in a twin-screw extruder. Insoluble and soluble fiber contents in extrudates increased as the proportions of amaranth and flaxseed increased. However, the highest flaxseed proportion had the highest soluble fiber content (1.9%). Extruded products with the highest proportion of flaxseed and amaranth resulted in the highest dietary fiber content and hardness values (5.2 N), which was correlated with the microstructural analysis where the crystallinity increased, resulting in larger, and more compact laminar structure. The extruded products with the highest maize grits proportion had the highest viscosity, expansion, and water absorption indexes, and the lowest water solubility index values. The mixtures with amaranth (18.7–22.9%), flaxseed (8.6–9.3%), and maize grits (63.8–67.3%) resulted in extruded products with acceptable physicochemical and functional properties.
For Whom and by Whom Children Are Named: Family Involvement in Contemporary Japanese Naming Practices
Authors: Unser-Schutz Giancarla
Published: May 2019

In pre-modern Japanese naming practices, familial relationships were frequently demonstrated systematically through personal names, but with changing lifestyles, family structures and naming trends, such systematic ways of creating familial ties through personal names have largely been lost. However, personal names may still express familial ties, but in different ways than in previous times. To consider this, this article utilizes a unique data set of 303 messages in municipal newsletters from parents about how they chose their child’s name, focusing on who was listed as choosing the name; whom the child was named for; and common elements within parent–child pairs and sibling sets. Parents themselves were most frequently noted to have selected the name, followed by the child’s older siblings; in comparison, grandparents were listed rarely. The use of a shared kanji ‘Chinese character’ between parents and children was also not common; however, it was more frequently observable in siblings’ names. Although the data set is small in size, the data strongly suggests that contemporary families are focused more on creating intragenerational connections between siblings, rather than intergenerational familial ties, which may be a result of the nuclearization of contemporary families.
The Domestic Tyranny of Haunted Houses in Mary Wilkins Freeman and Shirley Jackson
Authors: Junker Christine
Published: May 2019

Mary Wilkins Freeman and Shirley Jackson, though writing in different time periods, are both invested in recuperating domesticity and using their work to imagine what domesticity removed from the context of marriage and children can offer single women. Both authors assert that emplacement within domestic enclosure is essential to securing feminine subjectivity, but their haunted house narratives undermine that very emplacement. Freeman’s stories, “The Southwest Chamber” and “The Hall Bedroom” anticipate Jackson’s more well-known The Haunting of Hill House in the way that unruly domesticity threatens the female character’s emplacement. Their haunted house narratives show that neither Freeman nor Jackson, for all that they are subversive in some ways, wants to dissolve the traditional ideological constructs of domesticity; instead, they want these ideologies to work in the culturally promised patriarchal fashion. Reading their haunted house narratives together reveals the dynamics and tensions of a domesticity that is fluid, entangled, and vibrant and the feminist potential such sites engender, even if the characters and texts in question cannot fully realize that potential.
The Traditionalist Salafis’ Perspective and Discourse on Militant Jihād
Authors: Ali Mashail Haydar
Published: May 2019

This paper argues that the jihādi ideology draws upon the authentic and normative traditionalist salafi epistemology, which provides a moral and theological justification for militant jihād. In the current climate of political unrest and conflicts in the Middle East, maintaining a nexus between jihād and authentic Islam is crucial for traditionalist salafism in pursuit of authority, relevance and political interests. Through the misappropriation of the Meccan-Medinan trajectory and the concepts of nasẖ (abrogation) and ʾijmāʿ (consensus), the input of salafi scholars and preachers revitalizes the discourse around militant jihād as a legitimate vehicle for change and consequently propels jihādi ideology forward.
Psychomotor Abilities of Professional Handball Players
Authors: Przednowek Krzysztof , Śliż Maciej , Lenik Justyna , Dziadek Bartosz , Cieszkowski Stanisław , Lenik Paweł , Kopeć Dorota , Wardak Katarzyna , Przednowek Karolina H.
Published: May 2019

The main purpose of the paper was to evaluate selected psychomotor abilities of handball players depending on the competition class (league), position on the court, training seniority and the dominant hand. The study covered a group of 40 handball players (age: 24.02 ± 3.99), while 50 non-training men (age: 22.90 ± 1.13) formed the control group. Studies were performed using Test2Drive computer tests. The following four tests were used for measuring psychomotor fitness: simple reaction time test, choice reaction time test, hand-eye coordination test and spatial anticipation test. An analysis revealed that handball players had better reaction times and movement times than the control group. The league, position on the court, training seniority and the dominant upper limb were analysed for their impact on the reaction time and movement time in handball players. An analysis of psychomotor abilities of handball players with regard to the league revealed that in the majority of tests the Superliga players had a shorter reaction time than players in lower leagues.
Earthquake Information Extraction and Comparison from Different Sources Based on Web Text
Authors: Han Xuehua , Wang Juanle
Published: May 2019

Web text, using natural language to describe a disaster event, contains a considerable amount of disaster information. Automatic extraction from web text of this disaster information (e.g., time, location, casualties, and disaster losses) is an important supplement to conventional disaster monitoring data. This study extracted and compared the characteristics of earthquake disaster information from web news media reports (news reports) and online disaster reduction agency reports (professional reports). Using earthquakes in China from 2015 to 2017 as a case study, a series of rules were created for extracting earthquake event information, including temporal extraction rules, a location trigger dictionary, and an attribute trigger dictionary. The differences in characteristics of news reports and professional reports were investigated in terms of their quantity and spatiotemporal distribution through statistical analysis, geocoding, and kernel density estimation. The information extracted from each set of reports was also compared with authoritative data. The results indicated that news reports are more extensive and have richer information. In contrast, professional reports are less repetitive as well as more accurate and standardized, mainly focusing on earthquakes with Ms ≥ 4 and/or earthquakes that may cause damage. These characteristics of disaster information from different web texts sources can be used to improve the efficiency and analysis of disaster information extraction. In addition, the rule-based approach proposed herein was found to be an accurate and viable way to extract earthquake information from web texts. The approach provided the technical basics and background information to support further research seeking human-centric disaster information, which cannot be acquired using traditional instrument monitoring methods, from web text.
Eye-tracking Evaluation of Weather Web Maps
Authors: Popelka Stanislav , Vondrakova Alena , Hujnakova Petra
Published: May 2019

Weather is one of the things that interest almost everyone. Weather maps are therefore widely used and many users use them in everyday life. To identify the potential usability problems of weather web maps, the presented research was conducted. Five weather maps were selected for an eye-tracking experiment based on the results of an online questionnaire: DarkSky, In-Počasí, Windy, YR.no, and Wundermap. The experiment was conducted with 34 respondents and consisted of introductory, dynamic, and static sections. A qualitative and quantitative analysis of recorded data was performed together with a think-aloud protocol. The main part of the paper describes the results of the eye-tracking experiment and the implemented research, which identify the strengths and weaknesses of the evaluated weather web maps and point out the differences between strategies in using maps by the respondents. The results include findings such as the following: users worked with web maps in the simplest form and they did not look for hidden functions in the menu or attempt to find any advanced functionality; if expandable control panels were available, the respondents only looked at them after they had examined other elements; map interactivity was not an obstacle unless it contained too much information or options to choose from; searching was quicker in static menus that respondents did not have to switch on or off; the graphic design significantly influenced respondents and their work with the web maps. The results of the work may be useful for further scientific research on weather web maps and related user issues.
The Cytoskeletal Protein Cyclase-Associated Protein 1 (CAP1) in Breast Cancer: Context-Dependent Roles in Both the Invasiveness and Proliferation of Cancer Cells and Underlying Cell Signals
Authors: Hasan Rokib , Zhou Guo-Lei
Published: May 2019

As a conserved actin-regulating protein, CAP (adenylyl Cyclase-Associated Protein) functions to facilitate the rearrangement of the actin cytoskeleton. The ubiquitously expressed isoform CAP1 drives mammalian cell migration, and accordingly, most studies on the involvement of CAP1 in human cancers have largely been based on the rationale that up-regulated CAP1 will stimulate cancer cell migration and invasiveness. While findings from some studies reported so far support this case, lines of evidence largely from our recent studies point to a more complex and profound role for CAP1 in the invasiveness of cancer cells, where the potential activation of cell adhesion signaling is believed to play a key role. Moreover, CAP1 was also found to control proliferation in breast cancer cells, through the regulation of ERK (External signal-Regulated Kinase). Alterations in the activities of FAK (Focal Adhesion Kinase) and ERK from CAP1 depletion that are consistent to the opposite adhesion and proliferation phenotypes were detected in the metastatic and non-metastatic breast cancer cells. In this review, we begin with the overview of the literature on CAP, by highlighting the molecular functions of mammalian CAP1 in regulating the actin cytoskeleton and cell adhesion. We will next discuss the role of the FAK/ERK axis, and possibly Rap1, in mediating CAP1 signals to control breast cancer cell adhesion, invasiveness, and proliferation, largely based on our latest findings. Finally, we will discuss the relevance of these novel mechanistic insights to ultimately realizing the translational potential of CAP1 in targeted therapeutics for breast cancer.
Innovative MRI Techniques in Neuroimaging Approaches for Cerebrovascular Diseases and Vascular Cognitive Impairment
Authors: Carnevale Lorenzo , Lembo Giuseppe
Published: May 2019

Cognitive impairment and dementia are recognized as major threats to public health. Many studies have shown the important role played by challenges to the cerebral vasculature and the neurovascular unit. To investigate the structural and functional characteristics of the brain, MRI has proven an invaluable tool for visualizing the internal organs of patients and analyzing the parameters related to neuronal activation and blood flow in vivo. Different strategies of imaging can be combined to obtain various parameters: (i) measures of cortical and subcortical structures (cortical thickness, subcortical structures volume); (ii) evaluation of microstructural characteristics of the white matter (fractional anisotropy, mean diffusivity); (iii) neuronal activation and synchronicity to identify functional networks across different regions (functional connectivity between specific regions, graph measures of specific nodes); and (iv) structure of the cerebral vasculature and its efficacy in irrorating the brain (main vessel diameter, cerebral perfusion). The high amount of data obtainable from multi-modal sources calls for methods of advanced analysis, like machine-learning algorithms that allow the discrimination of the most informative features, to comprehensively characterize the cerebrovascular network into specific and sensitive biomarkers. By using the same techniques of human imaging in pre-clinical research, we can also investigate the mechanisms underlying the pathophysiological alterations identified in patients by imaging, with the chance of looking for molecular mechanisms to recover the pathology or hamper its progression.
Editorial for Special Issue “Plant Genetics and Molecular Breeding”
Authors: Martínez-Gómez Pedro
Published: May 2019

The development of new plant varieties is a long and tedious process involving the generation of large seedling populations to select the best individuals [...]
The Role of gp91phox and the Effect of Tranexamic Acid Administration on Hair Color in Mice
Authors: Hiramoto Keiichi , Yamate Yurika , Takishita Yutaka , Sato Eisuke F.
Published: May 2019

We observed that on long-term breeding, gp91phox-knockout (gp91phox−/−) mice developed white hair. Here, we investigate the origin of this hitherto unexplained phenomenon. Moreover, we investigated the effect of tranexamic acid administration on the hair color in gp91phox−/− mice. We administered tranexamic acid (about 12 mg/kg/day) orally to 9-week-old C57BL/6j (control) and gp91phox−/− mice, thrice a week for 12 months. Compared to control mice, gp91phox−/− mice showed more white hair. However, the concentrations of reactive oxygen species and the levels of interleukin (IL)-1β and transforming growth factor (TGF)-β in the skin were lower than those in the control group. Furthermore, increase in white hair was observed in the control mice upon administration of the IL-1β antagonist. On the other hand, administration of tranexamic acid led to brown colored hair on gp91phox−/− mice. Although tranexamic acid treatment did not alter the expression levels of melanocortin receptor 1 and agouti signaling protein on hair follicles, it increased the expression of mahogunin ring finger protein 1 (MGRN1) and collagen XVII. These results suggested that retention of black hair requires the gp91phox/ROS/IL-1β/TGF-β pathway and that elevated levels of MGRN1 and collagen XVII lead to brown hair in gp91phox−/− mice.
The Role of Ubiquitination in Regulating Embryonic Stem Cell Maintenance and Cancer Development
Authors: Wang Dian , Bu Fan , Zhang Weiwei
Published: May 2019

Ubiquitination regulates nearly every aspect of cellular events in eukaryotes. It modifies intracellular proteins with 76-amino acid polypeptide ubiquitin (Ub) and destines them for proteolysis or activity alteration. Ubiquitination is generally achieved by a tri-enzyme machinery involving ubiquitin activating enzymes (E1), ubiquitin conjugating enzymes (E2) and ubiquitin ligases (E3). E1 activates Ub and transfers it to the active cysteine site of E2 via a transesterification reaction. E3 coordinates with E2 to mediate isopeptide bond formation between Ub and substrate protein. The E1-E2-E3 cascade can create diverse types of Ub modifications, hence effecting distinct outcomes on the substrate proteins. Dysregulation of ubiquitination results in severe consequences and human diseases. There include cancers, developmental defects and immune disorders. In this review, we provide an overview of the ubiquitination machinery and discuss the recent progresses in the ubiquitination-mediated regulation of embryonic stem cell maintenance and cancer biology.
Regulation of Dual-Specificity Phosphatase (DUSP) Ubiquitination and Protein Stability
Authors: Chen Hsueh-Fen , Chuang Huai-Chia , Tan Tse-Hua
Published: May 2019

Mitogen-activated protein kinases (MAPKs) are key regulators of signal transduction and cell responses. Abnormalities in MAPKs are associated with multiple diseases. Dual-specificity phosphatases (DUSPs) dephosphorylate many key signaling molecules, including MAPKs, leading to the regulation of duration, magnitude, or spatiotemporal profiles of MAPK activities. Hence, DUSPs need to be properly controlled. Protein post-translational modifications, such as ubiquitination, phosphorylation, methylation, and acetylation, play important roles in the regulation of protein stability and activity. Ubiquitination is critical for controlling protein degradation, activation, and interaction. For DUSPs, ubiquitination induces degradation of eight DUSPs, namely, DUSP1, DUSP4, DUSP5, DUSP6, DUSP7, DUSP8, DUSP9, and DUSP16. In addition, protein stability of DUSP2 and DUSP10 is enhanced by phosphorylation. Methylation-induced ubiquitination of DUSP14 stimulates its phosphatase activity. In this review, we summarize the knowledge of the regulation of DUSP stability and ubiquitination through post-translational modifications.
Hyperprogression under Immunotherapy
Authors: Frelaut Maxime , Le Tourneau Christophe , Borcoman Edith
Published: May 2019

Immunotherapy is now widely prescribed in oncology, leading to the observation of new types of responses, including rapid disease progression sometimes reported as hyperprogression. However, only a few studies have assessed the question of hyperprogression and there is no consensual definition of this phenomenon. We reviewed existing data on hyperprogression in published studies, focusing on reported definitions, predictive factors, and potential biological mechanisms. Seven studies retrospectively assessed hyperprogression incidence, using various definitions, some based on the tumoral burden variation across time with repeated computed-tomography (CT) scan, others based on an association of radiological and clinical criteria. Reported hyperprogression incidence varied between 4% and 29% of all responses, mostly in multi-tumor cohorts and with patients receiving immune checkpoint inhibitors. Hyperprogression correlated with worse chances of survival than standard progression in two studies. However, no strong predictive factors of hyperprogression were identified, and none were consistent across studies. In total, hyperprogression is a frequent pattern of response under immunotherapy, with a strong impact on patient outcome. There is a need for a consensual definition of hyperprogression. Immunotherapy should be stopped early in cases where there is suspicion of hyperprogression.
Clinical Improvements in Comorbid Gambling/Cocaine Use Disorder (GD/CUD) Patients Undergoing Repetitive Transcranial Magnetic Stimulation (rTMS)
Authors: Cardullo Stefano , Gomez Perez Luis Javier , Marconi Linda , Terraneo Alberto , Gallimberti Luigi , Bonci Antonello , Madeo Graziella
Published: May 2019

(1) Background: Pathological gambling behaviors may coexist with cocaine use disorder (CUD), underlying common pathogenic mechanisms. Repetitive transcranial magnetic stimulation (rTMS) has shown promise as a therapeutic intervention for CUD. In this case series, we evaluated the clinical effects of rTMS protocol stimulating the left dorsolateral prefrontal cortex (DLPFC) on the pattern of gambling and cocaine use. (2) Methods: Gambling severity, craving for cocaine, sleep, and other negative affect symptoms were recorded in seven patients with a diagnosis of gambling disorder (South Oaks Gambling Screen (SOGS) >5), in comorbidity with CUD, using the following scales: Gambling-Symptom Assessment Scale (G-SAS), Cocaine Craving Questionnaire (CCQ), Beck Depression Inventory-II (BDI-II), Self-rating Anxiety Scale (SAS), and Symptoms checklist-90 (SCL-90). The measures were assessed before the rTMS treatment and after 5, 30, and 60 days of treatment. Patterns of gambling and cocaine use were assessed by self-report and regular urine screens. (3) Results: Gambling severity at baseline ranged from mild to severe (mean ± Standard Error of the Mean (SEM), G-SAS score baseline: 24.42 ± 2.79). G-SAS scores significantly improved after treatment (G-SAS score Day 60: 2.66 ± 1.08). Compared to baseline, consistent improvements were significantly seen in craving for cocaine and in negative-affect symptoms. (4) Conclusions: The present findings provide unprecedent insights into the potential role of rTMS as a therapeutic intervention for reducing both gambling and cocaine use in patients with a dual diagnosis.
Effects of Bilateral Infraorbital and Infratrochlear Nerve Block on Emergence Agitation after Septorhinoplasty: A Randomized Controlled Trial
Authors: Choi Hoon , Jung Seung Ho , Hong Jin Myung , Joo Young Ho , Kim Youme , Hong Sang Hyun
Published: May 2019

Emergence agitation is common after septorhinoplasty, and postoperative pain is the main risk factor for this condition. Infraorbital and infratrochlear nerve block have been reported to facilitate pain management in patients after nasal procedures. The effect of peripheral nerve block on the incidence of emergence agitation has not been evaluated. Sixty-six patients that were scheduled for septorhinoplasty were assigned to receive bilateral infraorbital and infratrochlear nerve block with either 8 mL of 0.5% ropivacaine (Block group) or isotonic saline (Sham Block group). The incidence of emergence agitation was evaluated using the Riker sedation-agitation scale. Analgesic consumption, hemodynamic parameters, postoperative pain scores, adverse events, and patient satisfaction with analgesia were evaluated. The incidence of emergence agitation was lower in the Block group than in the Sham Block group (6 (20.0%) versus 20 (62.5%), p = 0.002). The mean intraoperative remifentanil consumption was lower in the Block group than in the Sham Block group (0.074 ± 0.014 μg/kg/min. versus 0.093 ± 0.019 μg/kg/min., respectively, p < 0.0001), as was the proportion of patients that needed postoperative tramadol administration and median postoperative pain score at 0–2 h after surgery (9 (30.0%) versus 21 (65.6%), p = 0.011; 3.0 (2.0–4.0) versus 4.0 (3.0–4.0), p < 0.0001, respectively). Hemodynamic parameters and the incidence of adverse events were similar between the two groups. The median patient satisfaction score with respect to analgesia was higher in the Block group than in the Sham Block group (3.5 (3.0–4.0) versus 3.0 (3.0–4.0), respectively, p = 0.034). The preoperative bilateral infraorbital and infratrochlear nerve block decreased the incidence of emergence agitation after septorhinoplasty.
Reiterative Mechanisms of Retinoic Acid Signaling during Vertebrate Heart Development
Authors: Perl Eliyahu , Waxman Joshua S.
Published: May 2019

Tightly-regulated levels of retinoic acid (RA) are critical for promoting normal vertebrate development. The extensive history of research on RA has shown that its proper regulation is essential for cardiac progenitor specification and organogenesis. Here, we discuss the roles of RA signaling and its establishment of networks that drive both early and later steps of normal vertebrate heart development. We focus on studies that highlight the drastic effects alternative levels of RA have on early cardiomyocyte (CM) specification and cardiac chamber morphogenesis, consequences of improper RA synthesis and degradation, and known effectors downstream of RA. We conclude with the implications of these findings to our understanding of cardiac regeneration and the etiologies of congenital heart defects.
Micro-Scale Surface Patterning of Titanium Dental Implants by Anodization in the Presence of Modifying Salts
Authors: Marenzi Gaetano , Spagnuolo Gianrico , Sammartino Josè Camilla , Gasparro Roberta , Rebaudi Alberto , Salerno Marco
Published: May 2019

The bone-implant interface influences peri-implant bone healing and osseointegration. Among various nano-engineering techniques used for titanium surface modification, anodization is a simple, high-throughput and low-cost process, resulting in a nanoporous oxide coating which can promote osseointegration and impart antimicrobial and immunomodulatory properties. We anodized rounded tip dental implants of commercial grade titanium in aqueous phosphoric acid modified with calcium and potassium acetate, and characterized the resulting surface morphology and composition with scanning electron microscopy and energy dispersive spectrometry. The appearance of nanopores on these implants confirmed successful nanoscale morphology modification. Additionally, the metal cations of the used salts were incorporated into the porous coating together with phosphate, which can be convenient for osseointegration. The proposed method for surface nanostructuring of titanium alloy could allow for fabrication of dental implants with improved biocompatibility in the next stage of research.
Quantifying the Adhesion of Silicate Glass–Ceramic Coatings onto Alumina for Biomedical Applications
Authors: Baino Francesco
Published: May 2019

Deposition of bioactive glass or ceramic coatings on the outer surface of joint prostheses is a valuable strategy to improve the osteointegration of implants and is typically produced using biocompatible but non-bioactive materials. Quantifying the coating–implant adhesion in terms of bonding strength and toughness is still a challenge to biomaterials scientists. In this work, wollastonite (CaSiO3)-containing glass–ceramic coatings were manufactured on alumina tiles by sinter-crystallization of SiO2–CaO–Na2O–Al2O3 glass powder, and it was observed that the bonding strength decreased from 34 to 10 MPa as the coating thickness increased from 50 to 300 µm. From the viewpoint of bonding strength, the coatings with thickness below 250 µm were considered suitable for biomedical applications according to current international standards. A mechanical model based on quantized fracture mechanics allowed estimating the fracture toughness of the coating on the basis of the experimental data from tensile tests. The critical strain energy release rate was also found to decrease from 1.86 to 0.10 J/m2 with the increase of coating thickness, which therefore plays a key role in determining the mechanical properties of the materials.
Superplasticity of Ti-6Al-4V Titanium Alloy: Microstructure Evolution and Constitutive Modelling
Authors: Mosleh Ahmed O. , Mikhaylovskaya Anastasia V. , Kotov Anton D. , Kwame James S. , Aksenov Sergey A.
Published: May 2019

Determining a desirable strain rate-temperature range for superplasticity and elongation-to-failure are critical concerns during the prediction of superplastic forming processes in α + β titanium-based alloys. This paper studies the superplastic deformation behaviour and related microstructural evolution of conventionally processed sheets of Ti-6Al-4V alloy in a strain rate range of 10–5–10–2 s–1 and a temperature range of 750–900 °C. Thermo-Calc calculation and microstructural analysis of the as-annealed samples were done in order to determine the α/β ratio and the grain size of the phases prior to the superplastic deformation. The strain rate ranges, which corresponds to the superplastic behaviour with strain rate sensitivity index m ˃ 0.3, are identified by step-by-step decreasing strain rate tests for various temperatures. Results of the uniaxial isothermal tensile tests at a constant strain rate range of 3 × 10−4–3 × 10−3 s−1 and a temperature range of 800–900 °C are presented and discussed. The experimental stress-strain data are utilized to construct constitutive models, with the purpose of predicting the flow stress behaviour of this alloy. The cross-validation approach is used to examine the predictability of the constructed models. The models exhibit excellent approximation and predictability of the flow behaviour of the studied alloy. Strain-induced changes in the grain structure are investigated by scanning electron microscopy and electron backscattered diffraction. Particular attention is paid to the comparison between the deformation behaviour and the microstructural evolution at 825 °C and 875 °C. Maximum elongation-to-failure of 635% and low residual cavitation were observed after a strain of 1.8 at 1 × 10−3 s−1 and 825 °C. This temperature provides 23 ± 4% β phase and a highly stable grain structure of both phases. The optimum deformation temperature obtained for the studied alloy is 825 °C, which is considered a comparatively low deformation temperature for the studied Ti-6Al-4V alloy.
Sandblasted and Acid Etched Titanium Dental Implant Surfaces Systematic Review and Confocal Microscopy Evaluation
Authors: Cervino Gabriele , Fiorillo Luca , Iannello Gaetano , Santonocito Dario , Risitano Giacomo , Cicciù Marco
Published: May 2019

The field of dental implantology has made progress in recent years, allowing safer and predictable oral rehabilitations. Surely the rehabilitation times have also been reduced, thanks to the advent of the new implant surfaces, which favour the osseointegration phases and allow the clinician to rehabilitate their patients earlier. To carry out this study, a search was conducted in the Pubmed, Embase and Elsevier databases; the articles initially obtained according to the keywords used numbered 283, and then subsequently reduced to 10 once the inclusion and exclusion criteria were applied. The review that has been carried out on this type of surface allows us to fully understand the features and above all to evaluate all the advantages or not related. The study materials also are supported by a manufacturing company, which provided all the indications regarding surface treatment and confocal microscopy scans. In conclusion, we can say that, thanks to these new surfaces, it has been possible to shorten the time necessary to obtain osseointegration and, therefore, secondary stability on the part of implants. The surfaces, therefore, guarantee an improved cellular adhesion and thanks to the excellent wettability all the biological processes that derive from it, such as increases in the exposed implant surface, resulting in an increase in bone-implant contact (BIC).
Basic Blue Dye Adsorption from Water Using Polyaniline/Magnetite (Fe3O4) Composites: Kinetic and Thermodynamic Aspects
Authors: Muhammad Amir , Shah Anwar-ul-Haq Ali , Bilal Salma , Rahman Gul
Published: May 2019

Owing to its exciting physicochemical properties and doping–dedoping chemistry, polyaniline (PANI) has emerged as a potential adsorbent for removal of dyes and heavy metals from aqueous solution. Herein, we report on the synthesis of PANI composites with magnetic oxide (Fe3O4) for efficient removal of Basic Blue 3 (BB3) dye from aqueous solution. PANI, Fe3O4, and their composites were characterized with several techniques and subsequently applied for adsorption of BB3. Effect of contact time, initial concentration of dye, pH, and ionic strength on adsorption behavior were systematically investigated. The data obtained were fitted into Langmuir, Frundlich, Dubbanin-Rudiskavich (D-R), and Tempkin adsorption isotherm models for evaluation of adsorption parameters. Langmuir isotherm fits closely to the adsorption data with R2 values of 0.9788, 0.9849, and 0.9985 for Fe3O4, PANI, and PANI/Fe3O4 composites, respectively. The maximum amount of dye adsorbed was 7.474, 47.977, and 78.13 mg/g for Fe3O4, PANI, and PANI/Fe3O4 composites, respectively. The enhanced adsorption capability of the composites is attributed to increase in surface area and pore volume of the hybrid materials. The adsorption followed pseudo second order kinetics with R2 values of 0.873, 0.979, and 0.999 for Fe3O4, PANI, and PANI/Fe3O4 composites, respectively. The activation energy, enthalpy, Gibbs free energy changes, and entropy changes were found to be 11.14, −32.84, −04.05, and −0.095 kJ/mol for Fe3O4, 11.97, −62.93, −07.78, and −0.18 kJ/mol for PANI and 09.94, −74.26, −10.63, and −0.210 kJ/mol for PANI/Fe3O4 respectively, which indicate the spontaneous and exothermic nature of the adsorption process.
Postmastectomy Radiation Therapy (PMRT) before and after 2-Stage Expander-Implant Breast Reconstruction: A Systematic Review
Authors: Oliver Jeremie D. , Boczar Daniel , Huayllani Maria T. , Restrepo David J. , Sisti Andrea , Manrique Oscar J. , Broer Peter Niclas , McLaughlin Sarah , Rinker Brian D. , Forte Antonio Jorge
Published: May 2019

Background: In those undergoing treatment for breast cancer, evidence has demonstrated a significant improvement in survival, and a reduction in the risk of local recurrence in patients who undergo postmastectomy radiation therapy (PMRT). There is uncertainty about the optimal timing of PMRT, whether it should be before or after tissue expander or permanent implant placement. This study aimed to summarize the data reported in the literature on the effect of the timing of PMRT, both preceding and following 2-stage expander-implant breast reconstruction (IBR), and to statistically analyze the impact of timing on infection rates and the need for explantation. Methods: A comprehensive systematic review of the literature was conducted using the PubMed/Medline, Ovid, and Cochrane databases without timeframe limitations. Articles included in the analysis were those reporting outcomes data of PMRT in IBR published from 2009 to 2017. Chi-square statistical analysis was performed to compare infection and explantation rates between the two subgroups at p < 0.05. Results: A total of 11 studies met the inclusion criteria for this study. These studies reported outcomes data for 1565 total 2-stage expander-IBR procedures, where PMRT was used (1145 before, and 420 after, implant placement). There was a statistically significant higher likelihood of infection following pre-implant placement PMRT (21.03%, p = 0.000079), compared to PMRT after implant placement (9.69%). There was no difference in the rate of explantation between pre-implant placement PMRT (12.93%) and postimplant placement PMRT (11.43%). Conclusion: This study suggests that patients receiving PMRT before implant placement in 2-stage expander–implant based reconstruction may have a higher risk of developing an infection.
Associations among Serum Lipocalin-2 Concentration, Human Papilloma Virus, and Clinical Stage of Cervical Cancer
Authors: Vitkauskaitė Agnė , Celiešiūtė Joana , Paškauskas Saulius , Skrodenienė Erika , Nadišauskienė Rūta Jolanta , Burkauskienė Aušra , Vaitkienė Daiva
Published: May 2019

Background and objective: Lipocalin 2 (LCN2) has an oncogenic role in promoting tumorigenesis through enhancing tumor cell proliferation and the metastatic potential. The aim of our study was to determine whether serum LCN2 could serve as a diagnostic marker of cervical cancer (CC) and to evaluate the correlation between its serum concentration, the clinical stage of the cancer and Human Papilloma Virus HPV infections in women. Materials and methods: A total of 33 women with histologically proven cervical cancer (CC), 9 women with high- grade cervical intraepithelial neoplasia (HSIL) and 48 healthy women (NILM) were involved in the study. A concentration of LCN2 was assayed with the Magnetic LuminexR Assay multiplex kit. An HPV genotyping kit was used for the detection and differentiation of 15 high-risk (HR) HPV types in the liquid-based cytology medium (LBCM) and the tissue biopsy. Results: The majority (84.8%) of the women were infected by HPV16 in the CC group, and there was no woman with HPV16 in the control group (P < 0.01). Several types of HR HPV were found more often in the LBCM compared to in the tissue biopsy (P = 0.044). HPV16 was more frequently detected in the tissue biopsy than the LBCM (P < 0.05). The LCN2 level was higher in HPV-positive than in HPV-negative women (P = 0.029). The LCN2 concentration was significantly higher in women with stage IV than those with stage I CC (P = 0.021). Conclusions: Many HR HPV types, together with HPV16/18, can colonize the vagina and cervix, but often HPV16 alone penetrates into the tissue and causes CC. The serum LCN2 concentration was found to be associated not only with HR HPV infection, irrespective of the degree of cervical intraepithelial changes, but also with advanced clinical CC stage. LCN2 could be used to identify patients with advanced disease, who require a more aggressive treatment.
Multifunctional Platforms Based on Graphene Oxide and Natural Products
Authors: Croitoru Alexa , Oprea Ovidiu , Nicoara Adrian , Trusca Roxana , Radu Mihai , Neacsu Ionela , Ficai Denisa , Ficai Anton , Andronescu Ecaterina
Published: May 2019

Background and objectives: In the last few years, graphene oxide has attracted much attention in biomedical applications due to its unique physico-chemical properties and can be used as a carrier for both hydrophilic and/or hydrophobic biomolecules. The purpose of this paper was to synthesize graphene oxide and to obtain multifunctional platforms based on graphene oxide as a nanocarrier loaded with few biologically active substances with anticancer, antimicrobial or anti-inflammatory properties such as gallic acid, caffeic acid, limonene and nutmeg and cembra pine essential oils. Materials and Methods: Graphene oxide was obtained according to the method developed by Hummers and further loaded with biologically active agents. The obtained platforms were characterized using FTIR, HPLC, TGA, SEM, TEM and Raman spectroscopy. Results: Gallic acid released 80% within 10 days but all the other biologically active agents did not release because their affinity for the graphene oxide support was higher than that of the phosphate buffer solution. SEM characterization showed the formation of nanosheets and a slight increase in the degree of agglomeration of the particles. The ratio I2D/IG for all samples was between 0.18 for GO-cembra pine and 0.27 for GO-limonene, indicating that the GO materials were in the form of multilayers. The individual GO sheets were found to have less than 20 µm, the thickness of GO was estimated to be ~4 nm and an interlayer spacing of about 2.12 Å. Raman spectroscopy indicated that the bioactive substances were adsorbed on the surface and no degradation occurred during loading. Conclusions: These findings encourage this research to further explore, both in vitro and in vivo, the biological activities of bioactive agents for their use in medicine.
Effects of Trace Erbium Addition on Microstructure and Mechanical Properties of Ti6Al4V-xEr Alloys
Authors: Wu Yakun , Guo Yanhua , Xu Guanglong , Chang Hui , Cui Yuwen
Published: May 2019

In order to discern the effect of rare earth element Er addition on grain refinement of the most widely used titanium alloy Ti6Al4V, new erbium modified Ti6Al4V alloys with compositions of Ti6Al4V-xEr (x = 0, 0.2, 0.4, 0.6 wt %) were developed and investigated for their microstructural characteristics and mechanical properties in comparison with their unmodified baseline alloy. Microstructural examinations revealed that, by adding Er, (1) the microstructure primarily retained a two-phase structure consisting of α and β, (2) remarkable grain refining occurred, and (3) some Er2O3 and Al2Er disperses were formed largely around the β phase and near the grain boundaries. Mechanical property measurements evidenced an overall enhancement under tension and hardness tests. An increase in both strength and plasticity with increasing Er content was obtained but followed by a drop, while a gradual monotonous improvement in hardness was achieved. The Ti6Al4V-0.2Er alloy exhibits optimal mechanical properties.
Feruloylated Arabinoxylans from Nixtamalized Maize Bran Byproduct: A Functional Ingredient in Frankfurter Sausages
Authors: Herrera-Balandrano Daniela D. , Báez-González Juan G. , Carvajal-Millán Elizabeth , Méndez-Zamora Gerardo , Urías-Orona Vania , Amaya-Guerra Carlos A. , Niño-Medina Guillermo
Published: May 2019

Feruloylated arabinoxylans obtained from nixtamalized maize bran were evaluated in terms of physicochemical characteristics and antioxidant capacity when incorporated in frankfurter sausages. Concentrations of 0.15% and 0.30% of feruloylated arabinoxylans were incorporated in frankfurter sausages formulations and a control without feruloylated arabinoxylans was also prepared. Shear force, hardness, color measurement, proximate analysis, pH, titratable acidity, water-holding capacity, total phenols, and antioxidant capacity were evaluated. Phenolic content and antioxidant capacity were significantly higher (P < 0.0001) in all treatments, sausages containing feruloylated arabinoxylans compared to the control. The results showed that there was a significant difference (P < 0.0001) in total phenolic content and antioxidant capacity with all feruloylated arabinoxylans sausages treatments higher than control. Additionally, significant differences (P < 0.0001) were obtained in the physicochemical parameters.
Investigation on the Cancer Invasion and Metastasis of Skin Squamous Cell Carcinoma by Raman Spectroscopy
Authors: Zhang Xu , Yu Fan , Li Jie , Song Dongliang , Li Heping , Wang Kaige , He Qingli , Wang Shuang
Published: May 2019

Raman spectroscopy facilitates accurate and minimally invasive investigation on biomedical samples to reveal their molecular-level biological information. In this work, the cancer field effects of squamous cell carcinoma (SCC) tissues were illustrated by Raman microspectroscopy. Referenced with hematoxylin and eosin (H&E) stained microscopic images, the biochemical variations during SCC progress were meticulously described by the Raman spectral features in different pathological areas of two lesion types, including the biochemical changes in collagen, lipids, DNA, and other components of SCC diffusion and metastasis. The experimental results demonstrated that the intensities of the Raman peaks representing collagen (853, 936, and 1248 cm−1) were decreased, whereas the intensities of peaks corresponding to DNA (720, 1327 cm−1) and lipids (1305 cm−1) were increased significantly in cancerous lesions, which testified that SCC originates from the epidermis and invades the dermis gradually. The achieved results not only described the molecular mechanism of skin carcinogenesis, but also provided vital reference data for in vivo skin cancer diagnosis using Raman spectroscopy.