bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimating the Mass of Food Components Necessary for the Utilization of Free Radical Particles in the Human Body
Authors: Gorbachev Victor , Nikulchev Evgeny , Kosenkov Alexander N. , Sokolov Andrey , Zavalishin Igor , Nikitin Igor
Published: November 2022

The article proposes an algorithm for an approximate assessment of the molar volume of free radicals generated in the human body per day. It takes into account the act of breathing, physical activity, food consumption, the influence of unfavorable environmental conditions, exposure to xenobiotics, as well as bad habits (alcohol and tobacco smoking). A calculation of the required set of the most commonly used food products for the disposal of free radicals was made. The calculation is a structure of four blocks with the possibility of adding optional data from human population genetic studies, environmental conditions, etc. In the proposed algorithm, the results of antiradical activity (ARA) of food products are used as input, including the results of predicting antiradical activity using artificial neural networks (ANN), which we published earlier. Based on the accepted values of one equivalent (in terms of the activity of 1 μmol of ascorbic acid), it was shown (for our data) that for the utilization of all free radicals produced in the human body per day, it will take an average of ≈260 to ≈540 g of food components in terms of dry mass (including proteins, fats, carbohydrates, etc.). At the same time, for the utilization of consumed xenobiotics, from 220 mg (in terms of vitamin C) to 260 mg (in terms of acetylcysteine -NAC) of additional plastic components or 11.5–13.0 g of essential amino acids will be required, which must be taken into account when calculating diets. This approach will be useful in the development of new functional foods, as well as in assessing the possible impact of diets on human health. Another applied point of this study is related to the possibility of using these data for better detailing and selection of food products for people working in conditions of increased radiation (in space conditions), in contact with harmful substances (chemical synthesis and production), for people practicing increased physical activity (bodybuilding and sports), and for the purposes of medical nutritional therapy.
Blockchain Technology for Electronic Health Records
Authors: Han Yujin , Zhang Yawei , Vermund Sten H.
Published: November 2022

Compared with traditional paper-based medical records, electronic health records (EHRs) are widely used because of their efficiency, security, and reducing data redundancy. However, EHRs still manifest poor interoperability and privacy issues are unresolved. As a distributed ledger protocol composed of encrypted blocks of data organized in chains, blockchain represents a potential tool to solve the shortcomings of EHRs in terms of interoperability and privacy. In this paper, we define EHRs and blockchain technology and introduce several classic schemes based on blockchain technology to strengthen EHR interoperability and privacy protection. We then review ongoing challenges in the areas of data management efficiency, fairness of access, and trust in the systems. In this commentary, we suggest ongoing research needs for health informatics, data sciences, and ethics to establish EHRs based on blockchain technology. Blockchain-based EHR schemes must address the potential inequality of healthcare resources, the huge carbon footprint of computational needs, and potential distrust of health providers and patients that may ensue with wider use of blockchain technology.
Dietary Exposure Assessment of Rare Earth Elements in the Chinese Population
Authors: Yang Daoyuan , Sui Haixia , Mao Weifeng , Wang Yibaina , Yang Dajin , Zhang Lei , Liu Zhaoping , Yong Ling , Song Yan
Published: November 2022

Rare earth elements (REEs) are widely found in foods. A high intake of REEs may have associations with adverse effects on human health. This study aimed to investigate the concentrations of REEs in foods in China and to assess the risk of dietary REEs exposure in the Chinese population. The mean concentrations of the total REEs in 27,457 food samples from 11 food categories ranged from 0.04 to 1.41 mg/kg. The daily mean dietary exposure of the total REEs was 1.62 μg/kg BW in the general Chinese population and ranged from 1.61 to 2.80 μg/kg BW in different sex–age groups. The high consumer exposure (95th percentile, P95) was 4.83 μg/kg BW, 9.38% of the temporary ADI (tADI) of REEs (51.5 μg/kg BW). None of the P95 exposure exceeded the tADI in all of the sub-groups. Lanthanum, cerium, and yttrium accounted for approximately 63% of the total exposure of the 16 REEs. The hazard index of 16 REEs was far below 1. Therefore, the health risk of dietary REEs exposure in the general Chinese population was low. No cumulative risk was found for the 16 REEs in China. The results indicate there was no need to stipulate the limits of REEs in foods.
Factors Associated with the Antibiotic Treatment of Children Hospitalized for COVID-19 during the Lockdown in Serbia
Authors: Prijić Andreja , Gazibara Tatjana , Prijić Sergej , Mandić-Rajčević Stefan , Maksimović Nataša
Published: November 2022

Unselective use of antibiotics to treat children with COVID-19 is one of the major issues during the pandemic in Serbia. Thus far, there has been no evidence about the predictors of multiple antibiotic use in the treatment of children with COVID-19. The purpose of this study was to assess the prevalence of antibiotic use, as well as to examine demographic and clinical factors associated with a greater number of antibiotics and with a longer antibiotic treatment administered to hospitalized children with COVID-19 during the lockdown in Serbia. This study included all children who were hospitalized from 6 March to 31 May 2020 at the only pediatric COVID-19 hospital, and who were confirmed to have SARS-CoV-2 infection. Demographic, clinical, and laboratory data were collected from medical records. The antibiotic treatment included the use of azithromycin, cephalosporin (ceftriaxone), ampicillin-amikacin, and hydroxychloroquine. The overall prevalence of antibiotics use in children hospitalized with COVID-19 regardless of age was 47.2% (43.3% in children aged 1–5 years and 44.4% in those aged 5–17 years). In children aged 1–5 years, not having a family member affected by COVID-19 (B = −1.38, 95% confidence interval [CI] −2.43, −0.34, p = 0.011), having pneumonia on chest X-ray (B = 0.81, 95%CI 0.34, 1.29, p = 0.002), being a boy (B = −0.65, 95%CI −1.17, −0.13, p = 0.018), and having higher C-reactive protein (CRP) values on admission (B = 0.12, 95%CI 0.07, 0.17, p = 0.001) were associated with the administration of a higher number of antibiotics. These factors, along with having fever (B = 3.20, 95%CI 1.03, 5.37, p = 0.006), were associated with a longer duration of antibiotic treatment in children aged 1–5 years. In children aged 5–17 years, having pharyngeal erythema (B = 1.37, 95%CI 0.61, 2.13, p = 0.001), fever (B = 0.43, 95%CI 0.07, 0.79, p = 0.018), and pneumonia on chest X-ray (B = 0.91, 95%CI 0.53, 1.29, p = 0.001), not having rhinorrhea (B = −1.27, 95%CI −2.47, −0.08, p = 0.037), being a girl (B = 0.52, 95%CI 0.08, 0.97, p = 0.021), and having higher CRP values on admission (B = 0.04, 95%CI 0.01, 0.06, p = 0.006) were associated with the administration of a higher number of antibiotics. These factors, not including the absence of rhinorrhea, were associated with a longer duration of antibiotics treatment in children aged 5–17 years. Demographic, epidemiological, clinical, and laboratory parameters were associated with the use of multiple antibiotics and a longer duration of antibiotic treatment both among children aged 1–5 years and those aged 5–17 years.
A Toolkit for Co-Designing towards Community-Based Active Ageing: Lessons Learned during Development
Authors: Wang Gubing , Kasraian Dena , Valk Carlijn , Lu Yuan , Hurst William , Jambroes Marielle , van Wesemael Pieter
Published: November 2022

It is increasingly recognized that community-based interventions for active ageing are more lasting and effective, yet the tools and methods for developing these interventions are lacking. This study investigates how to co-design community-based active ageing with older adults via the development of a toolkit to support this goal. Rapid reviews were conducted to understand (i) the effective behavioural change techniques for older adults, (ii) how to co-design with older adults for community-based interventions, and (iii) how to design tools for behaviour change that are easy to use. These reviews served as the foundation for developing a toolkit to support the co-design of community-based active ageing, which was evaluated during an interdisciplinary hackathon with older adults. Quantitative data from the surveys suggested that the confidence levels of students in developing interventions for health behaviour change and in co-designing with older adults increased after the hackathon, and the enjoyment of participating in the hackathon and of using the toolkit were statistically significant factors influencing this increase. Qualitative data from interviews and observations revealed how the toolkit was (un)used by the participants and what aspects of the toolkit can be improved. We encourage future researchers and practitioners to apply and adapt our research findings to the communities of older adults that they are working with.
‘This Is What the Colour Green Smells Like!’: Urban Forest Bathing Improved Adolescent Nature Connection and Wellbeing
Authors: McEwan Kirsten , Potter Vanessa , Kotera Yasuhiro , Jackson Jessica Eve , Greaves Sarah
Published: November 2022

Background: Research suggests that an early connection with nature can benefit wellbeing into adulthood. However, there is less research assessing whether adolescents benefit from formal nature connection interventions such as forest bathing (slow mindful nature walks). This research aimed to assess whether an urban nature connection intervention (called ParkBathe) could improve adolescents’ nature connection and wellbeing. Method: In an experimental repeated measures design, 44 adolescents sampled opportunistically from Scouts groups, completed surveys and interviews before and after experiencing an urban nature connection intervention. Results: Paired-samples t-tests between baseline and post-intervention survey scores revealed statistically significant improvements in anxiety (13% reduction); rumination (44% reduction); scepticism (17% reduction); nature connection (25% increase); and social connection (12% increase). The largest effect size was found for nature connection. Interviews revealed that before the session, participants had a mixed understanding and expectations of the intervention. Conclusions: After the session, the participants expressed enjoying the social aspects of being part of a group and being present in the moment by noticing nature. They expressed the effects of this as immediately calming and relaxing. Urban forest bathing improved nature connection and wellbeing in adolescents and could be implemented and/or signposted by schools and youth charities.
Household Factors of Foodborne Diarrhea in Children under Five in Two Districts of Maputo, Mozambique
Authors: Machava Nórgia Elsa , Mulaudzi Fhumulani Mavis , Salvador Elsa Maria
Published: November 2022

Household factors involved in the disease of diarrhea are multifaceted. This study aimed to explore and describe the household factors affecting foodborne diarrhea in children younger than 5 years old using structured questionnaire data based on quantitative tools. The sample size was calculated based on a binomial distribution. A total of 300 children, together with their caregivers, participated, and the data were descriptively and mathematically analyzed using Epi Info modelling. The caregivers were mostly female and included 93.3% rural and 84% urban dwellers of ages between 18 and 38, who were single but living with someone. Of the children who were under six months of age, 23.3% in rural areas and 16.6% in urban areas had diarrhea, while of the children between 12 and 23 months of age, 36.6% in urban areas and 30% in rural areas had diarrhea. The relatives had similar symptoms before the child became ill, with 12.6% of relatives in rural areas and 13.3% in urban areas reporting this. Before receiving medical assistance, 51.3% of children in rural areas and 16% of children in urban areas were treated with traditional medication. Water was not treated before drinking in 48% of rural cases and 45.3% of urban cases. A total of 24.6% of infants in urban areas and 12.6% of infants in rural areas used a bottle for feeding. The factors affecting foodborne diarrhea were the use of traditional medication in rural areas, bottle feeding in urban areas and untreated water used for drinking in both areas.
Characteristics and Statistical Analysis of Large and above Hazardous Chemical Accidents in China from 2000 to 2020
Authors: Yang Dingding , Zheng Yu , Peng Kai , Pan Lidong , Zheng Juan , Xie Baojing , Wang Bohong
Published: November 2022

To investigate the occurrence and development pattern of large-scale hazardous chemicals emergencies, a statistical analysis of 195 large and above accidents of hazardous chemicals in China during 2000–2020 was conducted. A general description of the characteristics of larger and above accidents based on statistical data was analyzed, and then the system risk of the hazardous chemical industry was calculated and evaluated by the entropy weight method and the TOPSIS method comprehensively. Results show that: (1) The geographical distribution of large and above hazardous chemical accidents (LAHCA) varies significantly; (2) The high-temperature season has high probabilities of having large and above accidents; (3) Human factors and management factors are the main causes of LAHCA; (4) During the period from 2000 to 2020, due to the rapid development of the chemical industry, the overall risk of accidents involving hazardous chemicals were upswing accompanied by volatility, and the risk of serious accidents remains high. The development history of safety regulations in China’s hazardous chemical sector and the industry’s projected course for future growth were then discussed. Finally, based on the findings of the aforementioned statistics and research, specific recommendations were provided for the safety management of the hazardous chemical sector. This study expects to provide a practical and effective reference for the construction of safety management as well as accident prevention in the hazardous chemical industry.
Economic Development and Gender Ratio Change in Chinese Suicide Rates (1990–2017)
Authors: Zhang Jie , Lyu Juncheng , Lamis Dorian A.
Published: November 2022

Objective: The overall gender ratio in Chinese suicide rates has substantially changed during the past three decades. In this study, we investigated the social economic factors and the mechanisms that may be contributing to this fluctuation. Study Design: This is a secondary analysis using suicide mortality data from the China Centers for Disease Control and Prevention. Methods: A statistical model was performed with province as the unit of analysis. The per capita GDP and income of each provincial-level region were collected from the Economic and Statistical Yearbook. Rate and ratio were used to describe the trend of variations, and correlation analyses were conducted to examine the association between economic development and gender ratio change. Results: The China overall male to female gender ratio of suicide rates increased as the GDP per capita grew (r = 0.439; p = 0.015). The gender ratio changed from 0.88 in 1990 to 1.56 in 2017, with the reversion point between 1995 and 2000. The most radical reverse changes in the gender ratios were found in large municipalities. Conclusions: Cultural and social economic variables may explain the gender ratio changes. Increased economic development has significantly reduced psychological strains on rural young women, which in turn decreased the suicide rate among this sub-population.
The Relationship between Inequalities in Household Sports Consumption Expenditures and Income Level
Authors: Paár Dávid , Pogátsa Zoltán , Ács Pongrác , Szentei András
Published: November 2022

Inequalities in income, wealth, quality of life, health and education are an intensively researched field of economics. In this study, we examine the inequality in sports expenditures of Hungarian households. We hypothesize that the development of income inequalities will also correlate significantly to inequalities in sports consumption, and this trend has been intensifying over the past two decades. The research is based on the Household Budget Survey database of Hungarian households for the period 2005–2017. The net income conditions of the population and the sports expenditure items recorded on the basis of the COICOP nomenclature are examined by income decile. Data is analysed using descriptive statistics, inequality indicators and correlation calculations. Aggregate household expenditures on passive sports consumption show a stagnant trend, while aggregate expenditures on active sports consumption follow a slightly upward trend among the Hungarian population. Inequality indicators show growing inequalities in terms of income and sports expenditure over the reviewed period. Income inequality and sports spending inequality move together. The Hungarian population is becoming polarised in terms of both income and level of sports expenditure.
Tirzepatide: A Systematic Update
Authors: Forzano Imma , Varzideh Fahimeh , Avvisato Roberta , Jankauskas Stanislovas S. , Mone Pasquale , Santulli Gaetano
Published: November 2022

Tirzepatide is a new molecule capable of controlling glucose blood levels by combining the dual agonism of Glucose-Dependent Insulinotropic Polypeptide (GIP) and Glucagon-Like Peptide-1 (GLP-1) receptors. GIP and GLP1 are incretin hormones: they are released in the intestine in response to nutrient intake and stimulate pancreatic beta cell activity secreting insulin. GIP and GLP1 also have other metabolic functions. GLP1, in particular, reduces food intake and delays gastric emptying. Moreover, Tirzepatide has been shown to improve blood pressure and to reduce Low-Density Lipoprotein (LDL) cholesterol and triglycerides. Tirzepatide efficacy and safety were assessed in a phase III SURPASS 1–5 clinical trial program. Recently, the Food and Drug Administration approved Tirzepatide subcutaneous injections as monotherapy or combination therapy, with diet and physical exercise, to achieve better glycemic blood levels in patients with diabetes. Other clinical trials are currently underway to evaluate its use in other diseases. The scientific interest toward this novel, first-in-class medication is rapidly increasing. In this comprehensive and systematic review, we summarize the main results of the clinical trials investigating Tirzepatide and the currently available meta-analyses, emphasizing novel insights into its adoption in clinical practice for diabetes and its future potential applications in cardiovascular medicine.
RNA-Seq and Genome-Wide Association Studies Reveal Potential Genes for Rice Seed Shattering
Authors: Wu Linxuan , Yue Jicheng , Wang Jiafeng , Lu Wenyu , Huang Ming , Guo Tao , Wang Hui
Published: November 2022

The loss of the shattering ability is one of the key events in rice domestication. The strength of the seed shattering ability is closely related to the harvest yield and the adaptability of modern mechanical harvesting methods. In this study, using a population of 587 natural rice cultivars, quantitative trait loci associated with seed shattering were detected by genome-wide association studies (GWASs). We consider the quantitative trait loci (QTLs) qBTS1 and qBTS3 to be the key loci for seed shattering in rice. Additionally, the abscission zone (AZ) and nonabscission zone (NAZ) of materials with a loss of shattering (DZ129) and easy shattering (W517) were subjected to RNA-Seq, and high-quality differential expression profiles were obtained. The AZ-specific differentially expressed genes (DEGs) of W517 were significantly enriched in plant hormone signal transduction, while the AZ-specific DEGs of DZ129 were enriched in phenylpropanoid biosynthesis. We identified candidate genes for the lignin-associated laccase precursor protein (LOC_Os01g63180) and the glycoside hydrolase family (LOC_Os03g14210) in the QTLs qBTS1 (chromosome 1) and qBTS3 (chromosome 3), respectively. In summary, our findings lay the foundation for the further cloning of qBTS1 and qBTS3, which would provide new insights into seed shattering in rice.
Role of YAP as a Mechanosensing Molecule in Stem Cells and Stem Cell-Derived Hematopoietic Cells
Authors: Damkham Nattaya , Issaragrisil Surapol , Lorthongpanich Chanchao
Published: November 2022

Yes-associated protein (YAP) and WW domain-containing transcription regulator protein 1 (WWTR1, also known as TAZ) are transcriptional coactivators in the Hippo signaling pathway. Both are well-known regulators of cell proliferation and organ size control, and they have significant roles in promoting cell proliferation and differentiation. The roles of YAP and TAZ in stem cell pluripotency and differentiation have been extensively studied. However, the upstream mediators of YAP and TAZ are not well understood. Recently, a novel role of YAP in mechanosensing and mechanotransduction has been reported. The present review updates information on the regulation of YAP by mechanical cues such as extracellular matrix stiffness, fluid shear stress, and actin cytoskeleton tension in stem cell behaviors and differentiation. The review explores mesenchymal stem cell fate decisions, pluripotent stem cells (PSCs), self-renewal, pluripotency, and differentiation to blood products. Understanding how cells sense their microenvironment or niche and mimic those microenvironments in vitro could improve the efficiency of producing stem cell products and the efficacy of the products.
Impact of Amyloid Pathology in Mild Cognitive Impairment Subjects: The Longitudinal Cognition and Surface Morphometry Data
Authors: Chang Hsin-I , Hsu Shih-Wei , Kao Zih-Kai , Lee Chen-Chang , Huang Shu-Hua , Lin Ching-Heng , Liu Mu-N , Chang Chiung-Chih
Published: November 2022

The amyloid framework forms the central medical theory related to Alzheimer disease (AD), and the in vivo demonstration of amyloid positivity is essential for diagnosing AD. On the basis of a longitudinal cohort design, the study investigated clinical progressive patterns by obtaining cognitive and structural measurements from a group of patients with amnestic mild cognitive impairment (MCI); the measurements were classified by the positivity (Aβ+) or absence (Aβ−) of the amyloid biomarker. We enrolled 185 patients (64 controls, 121 patients with MCI). The patients with MCI were classified into two groups on the basis of their [18F]flubetaben or [18F]florbetapir amyloid positron-emission tomography scan (Aβ+ vs. Aβ−, 67 vs. 54 patients) results. Data from annual cognitive measurements and three-dimensional T1 magnetic resonance imaging scans were used for between-group comparisons. To obtain longitudinal cognitive test scores, generalized estimating equations were applied. A linear mixed effects model was used to compare the time effect of cortical thickness degeneration. The cognitive decline trajectory of the Aβ+ group was obvious, whereas the Aβ− and control groups did not exhibit a noticeable decline over time. The group effects of cortical thickness indicated decreased entorhinal cortex in the Aβ+ group and supramarginal gyrus in the Aβ− group. The topology of neurodegeneration in the Aβ− group was emphasized in posterior cortical regions. A comparison of the changes in the Aβ+ and Aβ− groups over time revealed a higher rate of cortical thickness decline in the Aβ+ group than in the Aβ− group in the default mode network. The Aβ+ and Aβ− groups experienced different APOE ε4 effects. For cortical–cognitive correlations, the regions associated with cognitive decline in the Aβ+ group were mainly localized in the perisylvian and anterior cingulate regions. By contrast, the degenerative topography of Aβ− MCI was scattered. The memory learning curves, cognitive decline patterns, and cortical degeneration topographies of the two MCI groups were revealed to be different, suggesting a difference in pathophysiology. Longitudinal analysis may help to differentiate between these two MCI groups if biomarker access is unavailable in clinical settings.
Distant Recurrence of a Cerebral Cavernous Malformation in the Vicinity of a Developmental Venous Anomaly: Case Report of Local Oxy-Inflammatory Events
Authors: Bianconi Andrea , Salvati Luca Francesco , Perrelli Andrea , Ferraris Chiara , Massara Armando , Minardi Massimiliano , Aruta Gelsomina , Rosso Miriam , Massa Micon Barbara , Garbossa Diego , Retta Saverio Francesco
Published: November 2022

Background: Cerebral cavernous malformations (CCMs) are a major type of cerebrovascular lesions of proven genetic origin that occur in either sporadic (sCCM) or familial (fCCM) forms, the latter being inherited as an autosomal dominant condition linked to loss-of-function mutations in three known CCM genes. In contrast to fCCMs, sCCMs are rarely linked to mutations in CCM genes and are instead commonly and peculiarly associated with developmental venous anomalies (DVAs), suggesting distinct origins and common pathogenic mechanisms. Case report: A hemorrhagic sCCM in the right frontal lobe of the brain was surgically excised from a symptomatic 3 year old patient, preserving intact and pervious the associated DVA. MRI follow-up examination performed periodically up to 15 years after neurosurgery intervention demonstrated complete removal of the CCM lesion and no residual or relapse signs. However, 18 years after surgery, the patient experienced acute episodes of paresthesia due to a distant recurrence of a new hemorrhagic CCM lesion located within the same area as the previous one. A new surgical intervention was, therefore, necessary, which was again limited to the CCM without affecting the pre-existing DVA. Subsequent follow-up examination by contrast-enhanced MRI evidenced a persistent pattern of signal-intensity abnormalities in the bed of the DVA, including hyperintense gliotic areas, suggesting chronic inflammatory conditions. Conclusions: This case report highlights the possibility of long-term distant recurrence of hemorrhagic sCCMs associated with a DVA, suggesting that such recurrence is secondary to focal sterile inflammatory conditions generated by the DVA.
In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A
Authors: Benko Filip , Fialková Veronika , Žiarovská Jana , Ďuračka Michal , Lukáč Norbert , Tvrdá Eva
Published: November 2022

Since the molecular similarities and differences among physiological capacitation and cryocapacitation have not been studied in detail, this study was designed to assess the gene and protein expression levels of the Cation channel of sperm (CatSper) 1 and 2, sodium bicarbonate (Na+/HCO3−) cotransporter (NBC) and protein kinase A (PKA) in un-capacitated (control), in vitro capacitated (CAP) and cryopreserved (CRYO) bovine spermatozoa. All samples were subjected to motility evaluation using the computer assisted sperm analysis and chlortetracycline (CTC) assay for the assessment of the capacitation patterns. Furthermore, quantitative reverse transcription PCR (qRT-PCR) and Western blots were used to monitor the expression patterns of the selected capacitation markers. The results showed a significant reduction in the gene and protein expression levels of CatSper1 and 2 in the CRYO group when compared to the CAP group (p < 0.0001). In the case of NBC, the results were not significantly different or were inconclusive. While a non-significant down-regulation of PKA was found in the CRYO group, a significant reduction in the expression of the PKA protein was found in frozen-thawed spermatozoa in comparison to the CAP group (p < 0.05). In conclusion, we may hypothesize that while in vitro capacitated and cryopreserved spermatozoa exhibit CTC-patterns consistent with capacitation events, the molecular machinery underlying CTC-positivity may be different.
Pathophysiology of Type 1 Diabetes and Gut Microbiota Role
Authors: Del Chierico Federica , Rapini Novella , Deodati Annalisa , Matteoli Maria Cristina , Cianfarani Stefano , Putignani Lorenza
Published: November 2022

Type 1 diabetes (T1D) is a multifactorial autoimmune disease driven by T-cells against the insulin-producing islet β-cells, resulting in a marked loss of β-cell mass and function. Although a genetic predisposal increases susceptibility, the role of epigenetic and environmental factors seems to be much more significant. A dysbiotic gut microbial profile has been associated with T1D patients. Moreover, new evidence propose that perturbation in gut microbiota may influence the T1D onset and progression. One of the prominent features in clinically silent phase before the onset of T1D is the presence of a microbiota characterized by low numbers of commensals butyrate producers, thus negatively influencing the gut permeability. The loss of gut permeability leads to the translocation of microbes and microbial metabolites and could lead to the activation of immune cells. Moreover, microbiota-based therapies to slow down disease progression or reverse T1D have shown promising results. Starting from this evidence, the correction of dysbiosis in early life of genetically susceptible individuals could help in promoting immune tolerance and thus in reducing the autoantibodies production. This review summarizes the associations between gut microbiota and T1D for future therapeutic perspectives and other exciting areas of research.
Tear Proteome Revealed Association of S100A Family Proteins and Mesothelin with Thrombosis in Elderly Patients with Retinal Vein Occlusion
Authors: Stepanov Alexander , Usharova Svetlana A. , Malsagova Kristina A. , Moshetova Larisa K. , Turkina Ksenia I. , Kopylov Arthur T. , Kaysheva Anna L.
Published: November 2022

Tear samples collected from patients with central retinal vein occlusion (CRVO; n = 28) and healthy volunteers (n = 29) were analyzed using a proteomic label-free absolute quantitative approach. A large proportion (458 proteins with a frequency > 0.6) of tear proteomes was found to be shared between the study groups. Comparative proteomic analysis revealed 29 proteins (p < 0.05) significantly differed between CRVO patients and the control group. Among them, S100A6 (log (2) FC = 1.11, p < 0.001), S100A8 (log (2) FC = 2.45, p < 0.001), S100A9 (log2 (FC) = 2.08, p < 0.001), and mesothelin ((log2 (FC) = 0.82, p < 0.001) were the most abundantly represented upregulated proteins, and β2-microglobulin was the most downregulated protein (log2 (FC) = −2.13, p < 0.001). The selected up- and downregulated proteins were gathered to customize a map of CRVO-related critical protein interactions with quantitative properties. The customized map (FDR < 0.01) revealed inflammation, impairment of retinal hemostasis, and immune response as the main set of processes associated with CRVO ischemic condition. The semantic analysis displayed the prevalence of core biological processes covering dysregulation of mitochondrial organization and utilization of improperly or topologically incorrect folded proteins as a consequence of oxidative stress, and escalating of the ischemic condition caused by the local retinal hemostasis dysregulation. The most significantly different proteins (S100A6, S100A8, S100A9, MSLN, and β2-microglobulin) were applied for the ROC analysis, and their AUC varied from 0.772 to 0.952, suggesting probable association with the CRVO.
Induction of Innate Memory in Human Monocytes Exposed to Mixtures of Bacterial Agents and Nanoparticles
Authors: Della Camera Giacomo , Liu Tinghao , Yang Wenjie , Li Yang , Puntes Victor F. , Gioria Sabrina , Italiani Paola , Boraschi Diana
Published: November 2022

We assessed whether concomitant exposure of human monocytes to bacterial agents and different engineered nanoparticles can affect the induction of protective innate memory, an immune mechanism that affords better resistance to diverse threatening challenges. Monocytes were exposed in vitro to nanoparticles of different chemical nature, shape and size either alone or admixed with LPS, and cell activation was assessed in terms of production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra). After return to baseline conditions, cells were re-challenged with LPS and their secondary “memory” response measured. Results show that nanoparticles alone are essentially unable to generate memory, while LPS induced a tolerance memory response (less inflammatory cytokines, equal or increased anti-inflammatory cytokines). LPS-induced tolerance was not significantly affected by the presence of nanoparticles during the memory generation phase, although with substantial donor-to-donor variability. This suggests that, despite the overall lack of significant effects on LPS-induced innate memory, nanoparticles may have donor-specific effects. Thus, future nanosafety assessment and nanotherapeutic strategies will need a personalized approach in order to ensure both the safety and efficacy of nano medical compounds for individual patients.
Hepatic Oleate Regulates Insulin-like Growth Factor-Binding Protein 1 Partially through the mTORC1-FGF21 Axis during High-Carbohydrate Feeding
Authors: O’Neill Lucas M. , Phang Yar Xin , Liu Zhaojin , Lewis Sarah A. , Aljohani Ahmed , McGahee Ayren , Wade Gina , Kalyesubula Mugagga , Simcox Judith , Ntambi James M.
Published: November 2022

Stearoyl-CoA desaturase-1 (SCD1) catalyzes the rate-liming step of monounsaturated fatty acid biosynthesis and is a key regulator of systemic glucose metabolism. Mice harboring either a global (GKO) or liver-specific deletion (LKO) of Scd1 display enhanced insulin signaling and whole-body glucose uptake. Additionally, GKO and LKO mice are protected from high-carbohydrate diet-induced obesity. Given that high-carbohydrate diets can lead to chronic metabolic diseases such as obesity, diabetes, and hepatic steatosis, it is critical to understand how Scd1 deficiency confers metabolically beneficial phenotypes. Here we show that insulin-like growth factor-binding protein 1 (IGFBP1), a hepatokine that has been reported to enhance insulin signaling, is significantly elevated in the liver and plasma of GKO and LKO mice fed a low-fat high-carbohydrate diet. We also observed that the expression of hepatic Igfbp1 is regulated by oleic acid (18:1n9), a product of SCD1, through the mTORC1-FGF21 axis both in vivo and in vitro.
Quality Control, Phytochemical Profile, and Antibacterial Effect of Origanum compactum Benth. Essential Oil from Morocco
Authors: Hayani Mouhcine , Bencheikh Noureddine , Ailli Atika , Bouhrim Mohamed , Elbouzidi Amine , Ouassou Hayat , Kharchoufa Loubna , Baraich Abdellah , Atbir Aziza , Ayyad Fatima Zahra , Drioiche Aziz , Addi Mohamed , Hano Christophe , Zair Touriya
Published: November 2022

Origanum compactum Benth (O. compactum) is widely used traditionally in Morocco to treat a broad range of illnesses, including infectious diseases. The aim of this study was to evaluate the phytochemical composition, quality control, and antibacterial activity of O. compactum leaf and flower essential oil. First, a quality control study on soil and irrigation water was performed to determine whether there was any risk of heavy metals endangering human health or causing stress to the plants studied. Laboratory examination of the environmental quality of the researched species revealed an almost absolute absence of metals that could endanger human health or any abiotic stressor. The essential oil was extracted by hydrodistillation. Chemical characterization was performed using gas chromatography-mass spectrometry (GC/MS). The yield of essential oil (EO) obtained by hydrodistillation of O. compactum leaves and flowers and moisture content were 4.27% and 12.20%, respectively. GC/MS identified 35 volatile compounds in the studied EO majorly composed of thymol (38.59%) followed by carvacrol (26.65%), o-cymene (14.33), and γ-terpinene (11.22%). The antibacterial activity of O. compactum leaf and flower essential oil was evaluated using the solid-state diffusion method against five Gram-negative bacterial strains and a Gram-positive strain. The results show that the essential oil of O. compactum leaves and flowers has a considerable inhibitory effect against E. coli with an MIC = 0.35 µg/mL, E. pseudocoloides (MIC = 0.35 µg/mL), E. vekanda (MIC = 0.35 µg/mL), K. pneumoniae (MIC = 0.7 µg/mL), P. aeruginosa (MIC = 0.35 µg/mL), and S. aureus (MIC = 0.35 µg/mL).
Behavior of Half-Joints: Design and Simulation of Laboratory Tests
Authors: Asso Rebecca , Domaneschi Marco , Marano Giuseppe C. , Palmisano Fabrizio , Palombella Giuseppe
Published: November 2022

European countries are characterized by an extensive infrastructural network, mainly built around the 1960s and 1970s. In that period prefabrication processes were starting to gain ground, and one of the most spread and studied typologies of bridges was constituted by reinforced or prestressed concrete decks. Those structures have gone through years of service, which caused the inevitable degradation of the materials and relevant deterioration of structural elements. Moreover, the design and construction processes of that period have soon become obsolete, and the knowledge relative to the influence of detailing increased significantly. One particular element that has been commonly used has been the half-joint, which is easy to prefabricate and has a strategic impact. However, in recent years this solution is showing critical aptitudes in resisting structural degradation and material decay. In addition, structural health monitoring (SHM) strategies are gaining attention since they are a very useful tool for gathering information on the current state of the structure and then for evaluating intervention plans to improve safety. Indeed, existing bridges, despite their working age, are still crucial to the development and sustainability of community life, and their decommissioning would be an act of critical impact on the communities (e.g., economy, logistics, sustainability). This contribution presents the design and the simulation of laboratory tests on half-joints of reinforced concrete beams that will be developed at the Politecnico di Torino in a subsequent step of the present research. They are designed to test and compare different monitoring techniques along with different steel reinforcement configurations. Specifically, the first part of the manuscript focuses on a review of the literature regarding the design, strengthening, and monitoring of half-joints. Subsequently, the laboratory setup to test half-joints is presented along with the numerical simulation to support the experimental design. Laboratory tests will involve the use of monitoring systems to detect the local response of the system and also to propose new solutions specifically for this type of connection using emerging technologies. Numerical collapse simulations show the effect of different reinforcement configurations and the collapse behavior.
Effects of Combination of Functional Electric Stimulation and Robotic Leg Movement Using Dynamic Tilt Table on Walking Characteristics in Post-Stroke Patients with Spastic Hemiplegia: A Randomized Crossover-Controlled Trial
Authors: Ueda Koki , Umemoto Yasunori , Kamijo Yoshi-ichiro , Sakurai Yuta , Araki Shohei , Ise Masato , Yoshioka Izumi , Banno Motohiko , Mochida Satoshi , Iwahashi Takaya , Shimokawa Toshio , Nishimura Yukihide , Tajima Fumihiro
Published: November 2022

Background: Spastic hemiplegia causes slow and unstable walking in post-stroke patients. Dynamic tilt table with robotic leg movement (DTTRLM) is safe and effective in improving walking. Functional electric stimulation (FES) improves walking speed in post-stroke patients with spastic hemiplegia. The aim of this study was to determine the effects of combined DTTRLM + FES on walking speed compared with DTTRLM alone. Methods: Twenty post-stroke patients were randomly assigned to receive either a single session of stepping + FES treatment or a single session of stepping alone treatment. After a one-week washout period, the same two groups underwent a single session of the other treatment, and the same measurements were taken. We measured walking speed, cadence, and the number of steps in a 10 m walking test (10MWT) and assessed Modified Ashworth Scale (MAS), Fugl–Meyer Assessment (FMA), and range of motion (ROM) before and after the intervention. Results: Stepping + FES significantly improved walking speed, number of steps, and ankle inversion ROM, compared with stepping alone. Adverse events were not observed in any subject. Conclusions: Robotic stepping therapy combined with FES significantly improved 10 m walking speed (10MWS) compared with stepping only in patients with post-stroke and spastic hemiplegia. Further studies are needed to determine the long-term effects of the combination treatment.
Associations between Insomnia, Daytime Sleepiness, and Depressive Symptoms in Adolescents: A Three-Wave Longitudinal Study
Authors: Liu Xianchen , Yang Yanyun , Liu Zhenzhen , Jia Cunxian
Published: November 2022

Background: Insomnia, daytime sleepiness, and depressive symptoms are prevalent in adolescents. This three-wave prospective study examined the associations between the three symptoms in adolescents. Methods: A total of 6995 schoolchildren in 7th and 10th grades (Mean age = 14.86 years) participated in a longitudinal study of behavior and health in Shandong, China. Standardized rating scales were used to assess symptoms of insomnia, daytime sleepiness, and depression in November–December in 2015, 1 year later, and 2 years later. Results: Insomnia was cross-sectionally associated with 10–14-fold increased odds of daytime sleepiness and 5–9-fold increased odds of depression. Daytime sleepiness was associated with 4–5-fold increased odds of depression. Insomnia, daytime sleepiness, or depression at a later time point was significantly predicted by itself at earlier time points. Insomnia was a significant predictor of daytime sleepiness and depression and a mediator between depression and daytime sleepiness. Daytime sleepiness was a significant predictor of insomnia and a mediator between depression and insomnia. Depression was a significant predictor of insomnia and daytime sleepiness and a mediator between insomnia and daytime sleepiness. Conclusions: Insomnia, daytime sleepiness, and depressive symptoms were highly comorbid in adolescents. The associations of insomnia with daytime sleepiness and depression were bidirectional. Depression predicted daytime sleepiness, but not vice versa. Further research is needed to understand the underlying neurobiological mechanisms between insomnia, daytime sleepiness, and depression during adolescence.
Kono-S Anastomosis in Crohn’s Disease: A Retrospective Study on Postoperative Morbidity and Disease Recurrence in Comparison to the Conventional Side-To-Side Anastomosis
Authors: Kelm Matthias , Reibetanz Joachim , Kim Mia , Schoettker Kathrin , Brand Markus , Meining Alexander , Germer Christoph-Thomas , Flemming Sven
Published: November 2022

Introduction: The rates of postoperative recurrence following ileocecal resection due to Crohn’s disease remain highly relevant. Despite this fact, while the Kono-S anastomosis technique initially demonstrated promising results, robust evidence is still lacking. This study aimed to analyze the short- and long-term outcomes of the Kono-S versus side-to-side anastomosis. Methods: A retrospective single-center study was performed including all patients who received an ileocecal resection between 1 January 2019 and 31 December 2021 at the Department of Surgery at the University Hospital of Wuerzburg. Patients who underwent conventional a side-to-side anastomosis were compared to those who received a Kono-S anastomosis. The short- and long-term outcomes were analyzed for all patients. Results: Here, 29 patients who underwent a conventional side-to-side anastomosis and 22 patients who underwent a Kono-S anastomosis were included. No differences were observed regarding short-term postoperative outcomes. The disease recurrence rate postoperatively was numerically lower following the Kono-S anastomosis (median Rutgeert score of 1.7 versus 2.5), with a relevantly increased rate of patients in remission (17.2% versus 31.8%); however, neither of these results reached statistical significance. Conclusion: The Kono-S anastomosis method is safe and feasible and potentially decreases the severity of postoperative disease remission.
Distal Radial Artery Access for Recanalization of Radial Artery Occlusion and Repeat Intervention: A Single Center Experience
Authors: Achim Alexandru , Kákonyi Kornél , Jambrik Zoltán , Olajos Dorottya , Nemes Attila , Bertrand Olivier F. , Ruzsa Zoltán
Published: November 2022

Background: Once occluded, the radial artery becomes unsuitable for repeat interventions and obligates the need for alternative vascular access, such as the femoral approach, which is not encouraged by current guidelines. With the dissemination of distal radial access (DRA), which allows the cannulation of the artery in its distal segment and which remains patent even in the case of radial artery occlusion (RAO), the option to perform angioplasty at this level becomes feasible. Methods: Thirty patients with RAO were enrolled in this pilot study. Recanalization was performed through DRA using hydrophilic guidewires. The feasibility endpoint was procedural success, namely the successful RAO recanalization, the efficacy endpoint was patency of the artery at 30 days, and the safety endpoint was the absence of periprocedural vascular major complications or major adverse cardiac and cerebrovascular events. Results: The mean age of the patients was 63 ± 11 years, and 15 patients (50%) were men. Most patients had asymptomatic RAO (n = 28, 93.3%), and only two (6.6%) reported numbness in their hands. The most common indication for the procedure was PCI (19, 63.2%). Total procedural time was 41 ± 22 min, while the amount of contrast used was 140 ± 28 mL. Procedural success was 100% (n = 30). Moreover, there were no major vascular complications (0%); only two small hematomas were described (10%) and one had an angiographically visible perforation (3%). One case of periprocedural stroke was reported (3%), with onset immediately after the procedure and recovering 24 h later. Twenty-seven radial arteries (90%) remained patent at the one-month follow-up. Conclusions: RAO recanalization is feasible and safe, and by using dedicated hydrophilic guidewires, the success rate is high without significantly increasing procedural time or the amount of used contrast.