bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and characterization of putative biomarkers and therapeutic axis in Glioblastoma multiforme microenvironment
Authors: Kumari Smita , Kumar Pravir
Published: July 2023

Non-cellular secretory components, including chemokines, cytokines, and growth factors in the tumor microenvironment, are often dysregulated, impacting tumorigenesis in Glioblastoma multiforme (GBM) microenvironment, where the prognostic significance of the current treatment remains unsatisfactory. Recent studies have demonstrated the potential of post-translational modifications (PTM) and their respective enzymes, such as acetylation and ubiquitination in GBM etiology through modulating signaling events. However, the relationship between non-cellular secretory components and post-translational modifications will create a research void in GBM therapeutics. Therefore, we aim to bridge the gap between non-cellular secretory components and PTM modifications through machine learning and computational biology approaches. Herein, we highlighted the importance of BMP1, CTSB, LOX, LOXL1, PLOD1, MMP9, SERPINE1, and SERPING1 in GBM etiology. Further, we demonstrated the positive relationship between the E2 conjugating enzymes (Ube2E1, Ube2H, Ube2J2, Ube2C, Ube2J2, and Ube2S), E3 ligases (VHL and GNB2L1) and substrate (HIF1A). Additionally, we reported the novel HAT1-induced acetylation sites of Ube2S (K211) and Ube2H (K8, K52). Structural and functional characterization of Ube2S (8) and Ube2H (1) have identified their association with protein kinases. Lastly, our results found a putative therapeutic axis HAT1-Ube2S(K211)-GNB2L1-HIF1A and potential predictive biomarkers (CTSB, HAT1, Ube2H, VHL, and GNB2L1) that play a critical role in GBM pathogenesis.
Visualization of c-di-GMP in multicellular Dictyostelium stages
Authors: Ide Hayato , Hayashida Yukihisa , Morimoto Yusuke V.
Published: July 2023

The bacterial signaling molecule cyclic diguanosine monophosphate (c-di-GMP) is only synthesized and utilized by the cellular slime mold Dictyostelium discoideum among eukaryotes. Dictyostelium cells undergo a transition from a unicellular to a multicellular state, ultimately forming a stalk and spores. While Dictyostelium is known to employ c-di-GMP to induce differentiation into stalk cells, there have been no reports of direct observation of c-di-GMP using fluorescent probes. In this study, we used a fluorescent probe used in bacteria to visualize its localization within Dictyostelium multicellular bodies. Cytosolic c-di-GMP concentrations were significantly higher at the tip of the multicellular body during stalk formation.
Possible Gardens: cosmopolitical worlds
Authors: Bragança Luciana Souza
Published: July 2023

This article presents some of the reflections produced by the Possible Gardens research, which explores the world of gardens where living beings interact directly, creating multispecific worlds. It is directed toward everyday gardens, which are still very present in Brazilian cities. It uses comparative case studies of multiple exemplar cases throughout the Arrudas River territory in the city of Belo Horizonte, Brazil. The aim is to present the contributions of the Possible Gardens, this expanded category of garden understood as cosmopolitical worlds, to the thinking of contemporary cities based on ecological practices derived from urban daily life. In addition, it opens an understanding of the potential of gardens as a culturally relevant element, as an example and catalyst for environmental policies.
Research progress on the reduced neural repair ability of aging Schwann cells
Authors: Zhang Hao , Zhang Zhong , Lin Haodong
Published: July 2023

Peripheral nerve injury (PNI) is associated with delayed repair of the injured nerves in elderly patients, resulting in loss of nerve function, chronic pain, muscle atrophy, and permanent disability. Therefore, the mechanism underlying the delayed repair of peripheral nerves in aging patients should be investigated. Schwann cells (SCs) play a crucial role in repairing PNI and regulating various nerve-repair genes after injury. SCs also promote peripheral nerve repair through various modalities, including mediating nerve demyelination, secreting neurotrophic factors, establishing Büngner bands, clearing axon and myelin debris, and promoting axon remyelination. However, aged SCs undergo structural and functional changes, leading to demyelination and dedifferentiation disorders, decreased secretion of neurotrophic factors, impaired clearance of axonal and myelin debris, and reduced capacity for axon remyelination. As a result, aged SCs may result in delayed repair of nerves after injury. This review article aimed to examine the mechanism underlying the diminished neural repair ability of aging SCs.
Txnip expression promotes JNK-mediated neuronal death in response to reactive oxygen species
Authors: García-Hernández Brenda , Morán Julio
Published: July 2023

TXNIP is a protein sensitive to oxidant conditions whose expression is related to the progression of death in cancer, diabetes, ischemia, and neurodegenerative diseases, among others. Because of this, many studies propose TXNIP as a therapeutic target in several diseases. Exposure of cerebellar granule neurons to staurosporine or low potassium leads to apoptotic death. Both conditions generate an early production of reactive oxygen species (ROS) that induces the activation of the ASK1 pathway and the apoptotic machinery. In these models, it has been shown an increase in TXNIP protein mediated by ROS. Here, we evaluated the molecular mechanisms involved in the regulation of the Txnip expression during neuronal death, as well as the role of the protein in the progression of cell death induced by these two apoptotic conditions. In cultured cerebellar granule neurons, we observed that low potassium and staurosporine induced an early increase in ROS that correlated with an increase in Txnip mRNA. When we evaluated the promoter of the gene, we found that the JASPAR-reported FOXO1/3 transcription factor motifs are close to the transcription start site (TSS). We then verified through the Chromatin immunoprecipitation technique (ChIP) that FOXO3 interacts with the Txnip promoter after 1 h of low potassium treatment. We also detected FOXO3 nuclear translocation by low potassium and staurosporine treatments. Finally, by using shRNA in the neuroblastoma MSN cell line, we found that Txnip downregulation decreased neuronal death induced by staurosporine stimulus. Together, these results suggest that ROS promotes the expression of Txnip through the activation of the FOXO3 transcription factor mediated by Akt inhibition. We also demonstrated that TXNIP is necessary for neuronal death progression.
Diffuse alveolar hemorrhage after hematopoietic cell transplantation- response to treatments and risk factors for mortality
Authors: Schoettler Michelle L. , Dandoy Christopher E. , Harris Anora , Chan Marilynn , Tarquinio Keiko M. , Jodele Sonata , Qayed Muna , Watkins Benjamin , Kamat Pradip , Petrillo Toni , Obordo Jeremy , Higham Christine S. , Dvorak Christopher C. , Westbrook Adrianna , Zinter Matt S. , Williams Kirsten M.
Published: July 2023

Diffuse alveolar hemorrhage (DAH) is a life-threatening complication of hematopoietic cellular therapy (HCT). This study aimed to evaluate the effect of DAH treatments on outcomes using data from consecutive HCT patients clinically diagnosed with DAH from 3 institutions between January 2018-August 2022. Endpoints included sustained complete response (sCR) defined as bleeding cessation without recurrent bleeding, and non-relapse mortality (NRM). Forty children developed DAH at a median of 56.5 days post-HCT (range 1-760). Thirty-five (88%) had at least one concurrent endothelial disorder, including transplant-associated thrombotic microangiopathy (n=30), sinusoidal obstructive syndrome (n=19), or acute graft versus host disease (n=10). Fifty percent had a concurrent pulmonary infection at the time of DAH. Common treatments included steroids (n=17, 25% sCR), inhaled tranexamic acid (INH TXA,n=26, 48% sCR), and inhaled recombinant activated factor VII (INH fVIIa, n=10, 73% sCR). NRM was 56% 100 days after first pulmonary bleed and 70% at 1 year. Steroid treatment was associated with increased risk of NRM (HR 2.25 95% CI 1.07-4.71, p=0.03), while treatment with INH TXA (HR 0.43, 95% CI 0.19- 0.96, p=0.04) and INH fVIIa (HR 0.22, 95% CI 0.07-0.62, p=0.005) were associated with decreased risk of NRM. Prospective studies are warranted to validate these findings.
Early prediction of atherosclerosis diagnosis with medical ambient intelligence
Authors: Yang Wen , Nie Qilin , Sun Yujie , Zou Danrong , Tang Jinmo , Wang Min
Published: July 2023

Atherosclerosis is a chronic vascular disease that poses a significant threat to human health. Common diagnostic methods mainly rely on active screening, which often misses the opportunity for early detection. To overcome this problem, this paper presents a novel medical ambient intelligence system for the early detection of atherosclerosis by leveraging clinical data from medical records. The system architecture includes clinical data extraction, transformation, normalization, feature selection, medical ambient computation, and predictive generation. However, the heterogeneity of examination items from different patients can degrade prediction performance. To enhance prediction performance, the “SEcond-order Classifier (SEC)” is proposed to undertake the medical ambient computation task. The first-order component and second-order cross-feature component are then consolidated and applied to the chosen feature matrix to learn the associations between the physical examination data, respectively. The prediction is lastly produced by aggregating the representations. Extensive experimental results reveal that the proposed method’s diagnostic prediction performance is superior to other state-of-the-art methods. Specifically, the Vitamin B12 indicator exhibits the strongest correlation with the early stage of atherosclerosis, while several known relevant biomarkers also demonstrate significant correlation in experimental data. The method proposed in this paper is a standalone tool, and its source code will be released in the future.
ASL MRI informs blood flow to chronic stroke lesions in patients with aphasia
Authors: Krishnamurthy Lisa C. , Glassman Clara , Han Joo H. , Song Serena E. , Denmon Chanse , Weatherill Maryanne , Rodriguez Amy D. , Crosson Bruce A. , Krishnamurthy Venkatagiri
Published: July 2023

Introduction: Response to post-stroke aphasia language rehabilitation is difficult to anticipate, mainly because few predictors can help identify optimal, individualized treatment options. Imaging techniques, such as Voxel-based Lesion Symptom Mapping have been useful in linking specific brain areas to language behavior; however, further development is required to optimize the use of structural and physiological information in guiding individualized treatment for persons with aphasia (PWA). In this study, we will determine if cerebral blood flow (CBF) mapped in patients with chronic strokes can be further used to understand stroke-related factors and behavior.

Methods: We collected perfusion MRI data using pseudo-Continuous Arterial Spin Labeling (pCASL) using a single post-labeling delay of 2,200 ms in 14 chronic PWA, along with high-resolution structural MRI to compute maps of tissue damage using Tissue Integrity Gradation via T2w T1w Ratio (TIGR). To quantify the CBF in chronic stroke lesions, we tested at what point spatial smoothing should be applied in the ASL analysis pipeline. We then related CBF to tissue damage, time since stroke, age, sex, and their respective cross-terms to further understand the variability in lesion CBF. Finally, we assessed the feasibility of computing multivariate brain-behavior maps using CBF and compared them to brain-behavior maps extracted with TIGR MRI.

Results: We found that the CBF in chronic stroke lesions is significantly reduced compared to its homologue grey and white matter regions. However, a reliable CBF signal (although smaller than expected) was detected to reveal a negative relationship between CBF and increasing tissue damage. Further, the relationship between the lesion CBF and age, sex, time since stroke, and tissue damage and cross-terms suggested an aging-by-disease interaction. This relationship was strongest when smoothing was applied in the template space. Finally, we show that whole-brain CBF relates to domain-general visuospatial functioning in PWA. The CBF-based brain-behavior maps provide unique and complementary information to structural (lesion-based) brain-behavior maps.

Discussion: Therefore, CBF can be detected in chronic stroke lesions using a standard pCASL MRI acquisition and is informative at the whole-brain level in identifying stroke rehabilitation targets in PWAs due to its relationship with demographic factors, stroke-related factors, and behavior.
Practice of pharmaceutical services and prescription analysis in internet-based psychiatric hospitals during COVID-19 pandemic in Wuxi, China
Authors: Du Zhiqiang , Jiang Ying , Lu Rongrong , Zhou Qin , Pan Yiting , Shen Yuan , Zhu Haohao
Published: July 2023

Objective

To study the practice of pharmaceutical services in internet-based psychiatric hospitals, and to analyze the prescriptions to ensure the safety and efficacy of internet-based medication in Wuxi, China.

Methods

All 1,259 internet-based prescriptions from our hospital in 2022 were collected, and data on patients’ age, gender, diagnosis, medications used, medication types, dosage forms, rationality of medication use, and reasons for irrationality were analyzed through descriptive statistics.

Results

In the electronic prescriptions of internet-based psychiatric hospitals, females accounted for the majority (64.50%), with a female-to-male ratio of 1.82:1. Middle-aged and young adults accounted for the majority of patients (57.50%). There were 47 diagnosed diseases involved, with 89 types of medications used and 1,938prescriptions issued. Among them, there were 78 types of western medicine with 1,876 prescriptions (96.80%), and 11 types of traditional Chinese medicine with 62 prescriptions (3.20%). The main medications used were anti-anxiety and antidepressant medications (44.94%) and psychiatric medications (42.21%). The dosage forms were all oral, with tablets (78.53%), capsules (17.54%), and solution preparations (2.17%) being the top three in frequency. According to the prescription review results, the initial pass rate of internet-based system review was 64.26%. After intervention by the internet-based system and manual review by pharmacist reviewers, the final pass rate of internet-based prescriptions reached 99.76%.

Conclusion

The practice of pharmaceutical services and prescription analysis in internet-based psychiatric hospitals could significantly improve medication rationality, which fills the research gap in this field. In addition, it promotes the transformation of pharmaceutical service models.
Relationships between internalized stigma and depression and suicide risk among queer youth in the United States: a systematic review and meta-analysis
Authors: Williams Denise Yookong , Hall William J. , Dawes Hayden C. , Srivastava Ankur , Radtke Spenser R. , Ramon Magdelene , Bouchard D. , Chen Wan-Ting , Goldbach Jeremy T.
Published: July 2023

Background

Queer youth experience high rates of depression and suicidality. These disparities stem from stigma-based stressors, including internalized stigma (i.e., negative social views that minoritized individuals internalize about their own identity). Given the importance of this factor in understanding mental health disparities among queer youth, we completed a systematic review and meta-analysis examining the relationships between internalized stigma and outcomes of depression and suicide risk (i.e., suicidal ideation, non-suicidal self-injury, and suicidal behavior).

Methods

We followed the PRISMA standards. Six bibliographic databases were searched for studies in the United States from September 2008 to March 2022. Dual independent screening of search results was performed based on a priori inclusion criteria.

Results

A total of 22 studies were included for data extraction and review. Most studies examined general internalized homophobia, with few examining internalized biphobia or transphobia. Many studies examined depression as an outcome, few studies examined suicidal ideation or behavior, and no studies examined non-suicidal self-injury. Meta-analyses model results show the association between general internalized queer stigma and depressive symptoms ranged r = 0.19, 95% CI [0.14, 0.25] to r = 0.24, 95% CI [0.19, 0.29], the latter reflecting more uniform measures of depression. The association between internalized transphobia and depressive outcomes was small and positive (r = 0.21, 95% CI [−0.24, 0.67]). General internalized queer stigma and suicidal ideation had a very weak positive association (r = 0.07, 95% CI [−0.27, 0.41]) and an even smaller, weaker positive association with suicide attempt (r = 0.02, 95% CI [0.01, 0.03]).

Conclusion

Implications for clinical practice, policy, and future research are discussed.
Determinants of postharvest losses along the baobab value chain in Malawi
Authors: Cossam Foster Geoffrey , Dzanja Joseph Kuyamba , Kamwana Bonnet , Olumeh Dennis Etemesi , Mithöfer Dagmar
Published: July 2023

Introduction

Wild fruits like Baobab are gaining status as a valuable food resource worldwide. As with other crops, the reduction of post-harvest losses is critical to enhancing sustainable utilisation of wild food resources. However, little information is documented on the magnitude and determinants of post-harvest losses (PHLs) amongst Non-Timber Forest Products (NTFPs), and baobab in particular.

Methods

This study used cross-sectional data collected from six districts to analyse PHLs along the baobab value chain in Malawi. A multistage sampling technique was used to sample 405 collectors, 96 traders, and 316 processors. Two-limit Tobit models were used to ascertain correlates of PHLs at each value chain level. The study quantified the value of PHLs and assessed the effect of socioeconomic factors on PHLs amongst baobab actors.

Results

We found that actors in the baobab value chain lose 7.78% of the total value of products held through PHLs. The results also showed that different sets of socioeconomic factors variably influenced PHLs amongst different value chain actors in the baobab value chain. For instance, gender was found to only correlate with PHLs amongst collectors. Whilst marital status was positively correlated with PHLs amongst collectors, and had a negative relationship amongst processors. PHLs at traders’ level are influenced by the number of people employed by an actor, the ability of customers to specify product attributes, and a proportional reduction in sales volume due to COVID-19. The study recommends the provision of training in PHLs management, and the formulating and enforcing of Baobab product handling standards.
Estimating the Cost of Wildlife Strikes in Australian Aviation Using Random Forest Modeling
Authors: Parsons Dan , Ryan Jason , Malouf Michael , Martin Wayne
Published: July 2023

Wildlife strikes in aviation represent a serious economic concern; however, in some jurisdictions, the costs associated with this phenomenon are not collected or shared. This hampers the industry’s ability to quantify the risk and assess the potential benefit from investment in effective wildlife hazard management activities. This research project has applied machine learning to the problem by training a random forest algorithm on wildlife strike cost data collected in the United States and predicting the costs associated with wildlife strikes in Australia. This method estimated a mean annual figure of AUD 7.9 million in repair costs and AUD 4.8 million in other costs from 2008 to 2017. It also provided year-on-year estimates showing variability through the reporting period that was not correlated with strike report numbers. This research provides a baseline figure for the Australian aviation industry to assess and review current and future wildlife hazard management practices. It also provides a technique for other countries, airlines, or airports to estimate the cost of wildlife strikes within their jurisdictions or operational environments.
Can Digital Finance Improve China’s Agricultural Green Total Factor Productivity?
Authors: Li Huiquan , Lin Qingning , Wang Yan , Mao Shiping
Published: July 2023

The improvement of agricultural green total factor productivity (AGTFP) is crucial to achieve sustainable agricultural development. By matching China’s provincial digital financial inclusion index and agricultural production data from 2011 to 2020, and on the basis of using the DEA–Malmquist productivity index to measure AGTFP, the fixed effect model and Mesomeric effect model are used to empirically test the impact and mechanism of digital financial development on China’s AGTFP. Our research found that from 2011 to 2020, China experienced consistent improvement in AGTFP, which was largely attributed to advancements in technology. Interestingly, the AGTFP in non-major grain-producing areas surpassed that in major grain producing areas. Additionally, digital finance has proven to be an effective tool in boosting China’s AGTFP, the coverage subdimension, the depth of use subdimension and the digitization subdimension all significantly promote the AGTFP. Digital finance can significantly promote the efficiency and progress of agricultural green technology, which shows that digital finance promotes AGTFP in a “dual wheel” driven manner. However, the impact of digital finance on agricultural green technology efficiency and progress is more pronounced in major grain-producing areas than in non-major areas. The impact mechanism demonstrates that digital finance has the potential to stimulate AGTFP in two key ways. First, it can improve the mismatch of agricultural production resources; second, it can promote agricultural technology innovation. Therefore, it is necessary to further promote the rapid development of digital finance, optimize the rational allocation of financial resources, and formulate tailored digital finance development strategies to promote green development of agriculture.
Analysis of Genetic Diversity and Population Structure of Tarim and Junggar Bactrian Camels Based on Simplified GBS Genome Sequencing
Authors: Tao Weikun , Aniwar Lazat , ZuliPicar Azat , Tulafu Hanikzi , Zhang Rongyin , Liu Bo , Wu Weiwei , Huang Juncheng
Published: July 2023

In view of the severe reduction in Bactrian camel germplasm resources, scientific evaluation, protection, and utilization is particularly important. Therefore, it is necessary to investigate the genetic diversity and genetic structure of this species, and identify the genes that have played important roles in its evolution. In this study, 21,971 SNPs were identified in 118 domestic Bactrian camels from the Tarim (n = 60) and Junggar (n = 58) populations using simplified GBS genome sequencing. The results show that Tarim and Junggar Bactrian camels have high nucleotide diversity. A phylogenetic tree constructed using structural analysis, principal component analysis (PCA), and the adjacency method (NJ) showed that Tarim and Junggar Bactrian camels were clustered together. The selection signals revealed that the Tarim and Junggar Bactrian camels shared 108 genes under positive selection, including WNT1, WNT10B, CD14, SEC61A2, DPAGT1, FOXO6, etc. These selected genes were widely involved in the immune system, embryonic development, lipid metabolism, and other processes. From a genomic analysis perspective, the genetic relationship between TLM and ZGE camels is close, with an average Fst of 0.048 and a relatively low average differentiation coefficient between the two populations. In addition, shared selected genes in the long-term depression pathway were significantly enriched in Tarim and Junggar. These findings will offer support and assistance for the exploration of genetic resource preservation, economically significant traits, and the mechanisms underlying biological characteristics, molecular breeding, and disease.
Copy Number Variation Regions Differing in Segregation Patterns Span Different Sets of Genes
Authors: Arias Katherine D. , Gutiérrez Juan Pablo , Fernández Iván , Álvarez Isabel , Goyache Félix
Published: July 2023

Copy number variations regions (CNVRs) can be classified either as segregating, when found in both parents, and offspring, or non-segregating. A total of 65 segregating and 31 non-segregating CNVRs identified in at least 10 individuals within a dense pedigree of the Gochu Asturcelta pig breed was subjected to enrichment and functional annotation analyses to ascertain their functional independence and importance. Enrichment analyses allowed us to annotate 1018 and 351 candidate genes within the bounds of the segregating and non-segregating CNVRs, respectively. The information retrieved suggested that the candidate genes spanned by segregating and non-segregating CNVRs were functionally independent. Functional annotation analyses allowed us to identify nine different significantly enriched functional annotation clusters (ACs) in segregating CNVR candidate genes mainly involved in immunity and regulation of the cell cycle. Up to five significantly enriched ACs, mainly involved in reproduction and meat quality, were identified in non-segregating CNVRs. The current analysis fits with previous reports suggesting that segregating CNVRs would explain performance at the population level, whereas non-segregating CNVRs could explain between-individuals differences in performance.
Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (Loxodonta africana)
Authors: Jansen van Vuuren Amèlia , Bolcaen Julie , Engelbrecht Monique , Burger Willem , De Kock Maryna , Durante Marco , Fisher Randall , Martínez-López Wilner , Miles Xanthene , Rahiman Farzana , Tinganelli Walter , Vandevoorde Charlot
Published: July 2023

Following population declines of the African savanna elephant (Loxodonta africana) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular and functional studies on the cancer suppression mechanisms of elephants, which have previously been linked to a redundancy of tumor suppressor gene TP53. This methodology describes the establishment of primary elephant dermal fibroblast (EDF) cell lines from skin punch biopsy samples (diameter: ±4 mm) of African savanna elephants (n = 4, 14–35 years). The applied tissue collection technique is minimally invasive and paves the way for future remote biopsy darting. On average, the first explant outgrowth was observed after 15.75 ± 6.30 days. The average doubling time (Td) was 93.02 ± 16.94 h and 52.39 ± 0.46 h at passage 1 and 4, respectively. Metaphase spreads confirmed the diploid number of 56 chromosomes. The successful establishment of EDF cell lines allows for future elephant cell characterization studies and for research on the cancer resistance mechanisms of elephants, which can be harnessed for human cancer prevention and treatment and contributes to the conservation of their genetic material.
Insertion of 643bp Retrotransposon Upstream of PPARγ CDS Is Associated with Backfat of Large White Pigs
Authors: He Jia , Yu Miao , Chi Chenglin , Du Zhanyu , Zheng Yao , Chen Cai , Moawad Ali Shoaib , Song Chengyi , Wang Xiaoyan
Published: July 2023

PPARs are essential regulators of mammalian fatty acid and lipid metabolism. Although the effects of genetic variations, including single nucleotide polymorphisms (SNPs) in PPARs genes on the phenotype of domestic animals have been investigated, there is limited information on the impact of retrotransposon insertion polymorphisms (RIPs). In this study, a combined comparative genome and polymerase chain reaction (PCR) was used to excavate the RIPs in porcine PPARs. We also investigated the potential effects of retrotransposon insertion on phenotype and expression patterns. This study identified the two RIPs in PPARs genes, namely an ERV in intron 1 of PPARα and a combined retrotransposon in intron 2 of PPARγ, designated as PPARα-ERV-RIP and PPARγ-COM-RIP, respectively. These RIPs exhibited different distribution patterns among Chinese indigenous breeds and Western commercial breeds. Individuals with the PPARα-ERV-RIP+/+ genotype (+/+ indicated homozygous with insertion) among Large White pigs had significantly higher (p < 0.05) corrected backfat thickness compared to those with the other two genotypes. Similarly, those with the PPARγ-COM-RIP−/− genotype had significantly higher (p < 0.05) corrected backfat thickness than those with the other two genotypes in Large White pigs. Moreover, in 30-day-old Sujiang piglets, the PPARγ gene expression in the backfat of those with the PPARγ-COM-RIP−/− genotype (−/− indicated homozygous without insertion) was significantly greater (p < 0.01) than those with other genotypes. The dual luciferase reporter gene assay demonstrated that the combined retrotransposon insertion significantly reduced the activity of the MYC promoter in both C2C12 and 3T3-L1 cells (p < 0.01). Therefore, the combined retrotransposon insertion could function as a repressor to decrease the expression of PPARγ, making PPARγ-COM-RIP a valuable molecular marker for assisted selection of backfat thickness in pig breeding.
Heat Stress as a Barrier to Successful Reproduction and Potential Alleviation Strategies in Cattle
Authors: Khan Imran , Mesalam Ayman , Heo Yun Seok , Lee Seo-Hyun , Nabi Ghulam , Kong Il-Keun
Published: July 2023

In recent decades, the adverse effects of global warming on all living beings have been unanimously recognized across the world. A high environmental temperature that increases the respiration and rectal temperature of cattle is called heat stress (HS), and it can affect both male and female reproductive functions. For successful reproduction and fertilization, mature and healthy oocytes are crucial; however, HS reduces the developmental competence of oocytes, which compromises reproduction. HS disturbs the hormonal balance that plays a crucial role in successful reproduction, particularly in reducing the luteinizing hormone and progesterone levels, which leads to severe problems such as poor follicle development with a poor-quality oocyte and problems related to maturity, silent estrus, abnormal or weak embryo development, and pregnancy loss, resulting in a declining reproduction rate and losses for the cattle industry. Lactating cattle are particularly susceptible to HS and, hence, their reproduction rate is substantially reduced. Additionally, bulls are also affected by HS; during summer, semen quality and sperm motility decline, leading to compromised reproduction. In summer, the conception rate is reduced by 20–30% worldwide. Although various techniques, such as the provision of water sprinklers, shade, and air conditioning, are used during summer, these methods are insufficient to recover the normal reproduction rate and, therefore, special attention is needed to improve reproductive efficiency and minimize the detrimental effect of HS on cattle during summer. The application of advanced reproductive technologies such as the production of embryos in vitro, cryopreservation during the hot season, embryo transfer, and timed artificial insemination may minimize the detrimental effects of HS on livestock reproduction and recover the losses in the cattle industry.
Evaluation of Electrical Cardiometry for Measuring Cardiac Output and Derived Hemodynamic Variables in Comparison with Lithium Dilution in Anesthetized Dogs
Authors: Paranjape Vaidehi V. , Garcia-Pereira Fernando L. , Menciotti Giulio , Saksena Siddharth , Henao-Guerrero Natalia , Ricco-Pereira Carolina H.
Published: July 2023

Numerous cardiac output (CO) technologies were developed to replace the ‘gold standard’ pulmonary artery thermodilution due to its invasiveness and the risks associated with it. Minimally invasive lithium dilution (LiD) shows excellent agreement with thermodilution and can be used as a reference standard in animals. This study evaluated CO via noninvasive electrical cardiometry (EC) and acquired hemodynamic variables against CO measured using LiD in six healthy, anesthetized dogs administered different treatments (dobutamine, esmolol, phenylephrine, and high-dose isoflurane) impacting CO values. These treatments were chosen to cause drastic variations in CO, so that fair comparisons between EC and LiD across a wide range of CO values (low, intermediate, and high) could be made. Statistical analysis included linear regression, Bland–Altman plots, Lin’s concordance correlation coefficient (ρc), and polar plots. Values of p < 0.05 represented significance. Good agreement was observed between EC and LiD, but consistent underestimation was noted when the CO values were high. The good trending ability, ρc of 0.88, and low percentage error of ±31% signified EC’s favorable performance. Other EC-acquired variables successfully tracked changes in CO measured using LiD. EC may be a pivotal hemodynamic tool for continuously monitoring circulatory changes, as well as guiding and treating cardiovascular anesthetic complications in clinical settings.
Impact of Surgical Lights on the Performance of Fluorescence-Guided Surgery Systems: A Pilot Study
Authors: Chiti Lavinia E. , Park Brian , d’Orchymont Faustine , Holland Jason P. , Nolff Mirja C.
Published: July 2023

Fluorescence-guided surgery can aid in the intraoperative visualization of target tissues, with promising applications in human and veterinary surgical oncology. The aim of this study was to evaluate the performances of two fluoresce camera systems, IC-FlowTM and VisionsenseTM VS3 Iridum, for the detection of two non-targeted (ICG and IRDye-800) and two targeted fluorophores (AngiostampTM and FAP-Cyan) under different room light conditions, including ambient light, new generation LED, and halogen artificial light sources, which are commonly used in operating theaters. Six dilutions of the fluorophores were imaged in phantom kits using the two camera systems. The limit of detection (LOD) and mean signal-to-background ratio (mSBR) were determined. The highest values of mSBR and a lower LOD were obtained in dark conditions for both systems. Under room lights, the capabilities decreased, but the mSBR remained greater than 3 (=clearly detectable signal). LOD and mSBR worsened under surgical lights for both camera systems, with a greater impact from halogen bulbs on VisionsenseTM VS3 Iridium and of the LED lights on IC-Flow due to a contribution of these lights in the near-infrared spectrum. When considering implementing FGS into the clinical routine, surgeons should cautiously evaluate the spectral contribution of the lights in the operating theater.
Contextualized Small Target Detection Network for Small Target Goat Face Detection
Authors: Wang Yaxin , Han Ding , Wang Liang , Guo Ying , Du Hongwei
Published: July 2023

With the advancement of deep learning technology, the importance of utilizing deep learning for livestock management is becoming increasingly evident. goat face detection provides a foundation for goat recognition and management. In this study, we proposed a novel neural network specifically designed for goat face object detection, addressing challenges such as low image resolution, small goat face targets, and indistinct features. By incorporating contextual information and feature-fusion complementation, our approach was compared with existing object detection networks using evaluation metrics such as F1-Score (F1), precision (P), recall (R), and average precision (AP). Our results show that there are 8.07%, 0.06, and 6.8% improvements in AP, P, and R, respectively. The findings confirm that the proposed object detection network effectively mitigates the impact of small targets in goat face detection, providing a solid basis for the development of intelligent management systems for modern livestock farms.
Compliance with a Procalcitonin-Based Protocol in Patients with Ventilation-Associated Pneumonia: An Observational, Retrospective Study
Authors: Rossi Matthieu , Delamarre Louis , Duclos Gary , Lakbar Ines , Hammad Emmanuelle , Arbelot Charlotte , Zieleskiewicz Laurent , Leone Marc
Published: July 2023

Background: Procalcitonin (PCT) protocols to guide antibiotic treatment for ventilator-associated pneumonia (VAP) in the intensive care unit aim at reducing antibiotic exposure. Our study goal was to measure compliance with a PCT protocol for VAP and to determine the associated variables. Methods: From 2017 to 2021, we conducted a retrospective, monocentric study including patients treated for VAP. In our PCT protocol, PCT was measured at the initiation of antibiotic treatment and every 48 h until treatment completion; antibiotics were stopped if PCT decreased by more than 80% from its highest value or fell below 0.5 ng/mL. We assessed the compliance with the PCT protocol and compared the compliant and noncompliant groups. Results: Among the 177 included patients, compliance with the PCT protocol was assessed at 58%. Noncompliance was due to lack of PCT measurements in 76% of cases. Compliance was higher in the medical patients (p = 0.04) and in those admitted for SARS-CoV-2 (p = 0.02). Compliance regarding the interruption of antibiotic therapy based on PCT was lower on weekends and holidays (p = 0.01). Outcomes did not differ according to compliance. Conclusion: This study assessed real-life compliance with the PCT protocol to monitor antibiotic treatment for VAP. Improving the measurement of PCT at the bedside would increase the rate.
Exploring the Potential of Bee-Derived Antioxidants for Maintaining Oral Hygiene and Dental Health: A Comprehensive Review
Authors: Choudhary Poonam , Tushir Surya , Bala Manju , Sharma Sanjula , Sangha Manjeet Kaur , Rani Heena , Yewle Nileshwari Raju , Kumar Parminder , Singla Diksha , Chandran Deepak , Kumar Manoj , Mekhemar Mohamed
Published: July 2023

Honey bee products comprise various compounds, including honey, propolis, royal jelly, bee pollen, bee wax and bee venom, which have long been recognized for their pharmacological and health-promoting benefits. Scientists have discovered that periodontal disorders stem from dental biofilm, an inflammatory response to bacterial overgrowth produced by dysbiosis in the oral microbiome. The bee products have been investigated for their role in prevention of oral diseases, which are attributed to a myriad of biologically active compounds including flavonoids (pinocembrin, catechin, caffeic acid phenethyl ester (CAPE) and galangin), phenolic acids (hydroxybenzoic acid, hydroxycinnamic acid, p-coumaric, ellagic, caffeic and ferulic acids) and terpenoids. This review aims to update the current understanding of role of selected bee products, namely, honey, propolis and royal jelly, in preventing oral diseases as well as their potential biological activities and mechanism of action in relation to oral health have been discussed. Furthermore, the safety of incorporation of bee products is also critically discussed. To summarize, bee products could potentially serve as a therapy option for people suffering from a variety of oral disorders.
Pepper Fruit Extracts Show Anti-Proliferative Activity against Tumor Cells Altering Their NADPH-Generating Dehydrogenase and Catalase Profiles
Authors: Rodríguez-Ruiz Marta , Ramos María C. , Campos María J. , Díaz-Sánchez Inmaculada , Cautain Bastien , Mackenzie Thomas A. , Vicente Francisca , Corpas Francisco J. , Palma José M.
Published: July 2023

Cancer is considered one of the main causes of human death worldwide, being characterized by an alteration of the oxidative metabolism. Many natural compounds from plant origin with anti-tumor attributes have been described. Among them, capsaicin, which is the molecule responsible for the pungency in hot pepper fruits, has been reported to show antioxidant, anti-inflammatory, and analgesic activities, as well as anti-proliferative properties against cancer. Thus, in this work, the potential anti-proliferative activity of pepper (Capsicum annuum L.) fruits from diverse varieties with different capsaicin contents (California < Piquillo < Padrón < Alegría riojana) against several tumor cell lines (lung, melanoma, hepatoma, colon, breast, pancreas, and prostate) has been investigated. The results showed that the capsaicin content in pepper fruits did not correspond with their anti-proliferative activity against tumor cell lines. By contrast, the greatest activity was promoted by the pepper tissues which contained the lowest capsaicin amount. This indicates that other compounds different from capsaicin have this anti-tumor potentiality in pepper fruits. Based on this, green fruits from the Alegría riojana variety, which has negligible capsaicin levels, was used to study the effect on the oxidative and redox metabolism of tumor cell lines from liver (Hep-G2) and pancreas (MIA PaCa-2). Different parameters from both lines treated with crude pepper fruit extracts were determined including protein nitration and protein S-nitrosation (two post-translational modifications (PTMs) promoted by nitric oxide), the antioxidant capacity, as well as the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX), among others. In addition, the activity of the NADPH-generating enzymes glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and NADP-isocitrate dehydrogenase (NADP-ICDH) was followed. Our data revealed that the treatment of both cell lines with pepper fruit extracts altered their antioxidant capacity, enhanced their catalase activity, and considerably reduced the activity of the NADPH-generating enzymes. As a consequence, less H2O2 and NADPH seem to be available to cells, thus avoiding cell proliferation and possibly triggering cell death in both cell lines.
High-Intensity Exercise Promotes Deleterious Cardiovascular Remodeling in a High-Cardiovascular-Risk Model: A Role for Oxidative Stress
Authors: Meza-Ramos Aline , Alcarraz Anna , Lazo-Rodriguez Marta , Sangüesa Gemma , Banon-Maneus Elisenda , Rovira Jordi , Ramirez-Bajo Maria Jose , Sitges Marta , Mont Lluís , Ventura-Aguiar Pedro , Batlle Montserrat , Guasch Eduard
Published: July 2023

Although the benefits of moderate exercise in patients at high cardiovascular risk are well established, the effects of strenuous exercise remain unknown. We aimed to study the impact of strenuous exercise in a very high cardiovascular risk model. Nephrectomized aged Zucker obese rats were trained at a moderate (MOD) or high (INT) intensity or were kept sedentary (SED) for 10 weeks. Subsequently, echocardiography and ex vivo vascular reactivity assays were performed, and blood, aortas, perivascular adipose tissue (PVAT), and left ventricles (LVs) were harvested. An improved risk profile consisting of decreased body weight and improved response to a glucose tolerance test was noted in the trained groups. Vascular reactivity experiments in the descending thoracic aorta demonstrated increased endothelial NO release in the MOD group but not in the INT group, compared with SED; the free radical scavenger TEMPOL improved endothelial function in INT rats to a similar level as MOD. An imbalance in the expression of oxidative stress-related genes toward a pro-oxidant environment was observed in the PVAT of INT rats. In the heart, INT training promoted eccentric hypertrophy and a mild reduction in ejection fraction. Obesity was associated with LV fibrosis and a transition toward β-myosin heavy chain and the N2Ba titin isoform. Exercise reverted the myosin imbalance, but only MOD reduced the predominance of the N2Ba titin isoform. In conclusion, moderate exercise yields the most intense cardiovascular benefits in a high-cardiovascular-risk animal model, while intense training partially reverts them.
Safety Analysis of Secondary Lining of Yulinzi Tunnel Based on Field Monitoring
Authors: Wu Yimin , Zhuang Guangzheng , Liang Weiming , Huang Le
Published: July 2023

In order to assess the safety of the secondary lining in water-rich Loess Tunnel, this study relies on the Yulinzi Tunnel project to continuously monitor the stress of the steel reinforcement in the secondary lining and analyze the temporal variation of the steel strain. Based on this data, the temporal variation and distribution characteristics of the internal forces of the structure were obtained through section force calculation. The safety factor of the secondary lining structure was evaluated by calculating the safety factor of the structure. It was found that the variation of the safety factor with time conforms to the exponential function, and the fitting results of different measurement points were good. This could be used to predict the future safety factor of the tunnel and evaluate its long-term safety, which has practical guidance significance for actual engineering projects. In addition, the long-term stress and structural deformation characteristics of the tunnel were obtained through numerical simulation.
AIM Triad: A Prioritization Strategy for Public Institutions to Improve Information Security Maturity
Authors: Hochstetter-Diez Jorge , Diéguez-Rebolledo Mauricio , Fenner-López Julio , Cachero Cristina
Published: July 2023

In today’s world, private and government organizations are legally obligated to prioritize their information security. They need to provide proof that they are continually improving their cybersecurity compliance. One approach that can help organizations achieve this goal is implementing information security maturity models. These models provide a structured framework for measuring performance and implementing best practices. However, choosing a suitable model can be challenging, requiring cultural, process, and work practice changes. Implementing multiple models can be overwhelming, if possible. This article proposes a prioritization strategy for public institutions that want to improve their information security maturity. We thoroughly analyzed various sources through systematic mapping to identify critical similarities in information security maturity models. Our research led us to create the AIM (Awareness, Infrastructure, and Management) Triad. This triad is a practical guide for organizations to achieve maturity in information security practices.
Detection of Broken Bars in Induction Motors Using Histogram Analysis of Current Signals
Authors: Hernandez-Ramirez Veronica , Almanza-Ojeda Dora-Luz , Cardenas-Cornejo Juan-Jose , Contreras-Hernandez Jose-Luis , Ibarra-Manzano Mario-Alberto
Published: July 2023

The lifetime of induction motors can be significantly extended by installing diagnostic systems for monitoring their operating conditions. In particular, detecting broken bar failures in motors is important for avoiding the risk of short circuits or other accidents with serious consequences. In the literature, many approaches have been proposed for motor fault detection; however, additional generalized methods based on local and statistical analysis could provide a low-complexity and feasible solution in this field of research. The proposed work presents a methodology for detecting one or two broken rotor bars using the sums and differences histograms (SDH) and machine learning classifiers in this context. From the SDH computed in one phase of the motor’s current, nine texture features are calculated for different displacements. Then, all features are used to train two classifiers and to find the best displacements for faults and health identification in the induction motors. A final experimental evaluation considering the best displacements shows an accuracy of 98.16% for the homogeneity feature and a few signal samples used in a decision tree classifier. Additionally, a polynomial regression curve validates the use of 50 samples to obtain an accuracy of 88.15%, whereas the highest performance is achieved for 250 samples.