bluuebunny/crossref_metadata_2025 huggingface dataset:
Stress induced phosphoprotein 1 overexpression controls proliferation, migration and invasion and is associated with poor survival in oral squamous cell carcinoma
Authors: Dourado Mauricio Rocha , Elseragy Amr , da Costa Bruno Cesar , Téo Fábio Haach , Guimarães Gustavo Narvaes , Machado Renato Assis , Risteli Maija , Wahbi Wafa , Gurgel Rocha Clarissa Araujo , Paranaíba Lívia Máris Ribeiro , González-Arriagada Wilfredo Alejandro , da Silva Sabrina Daniela , Rangel Ana Lucia Carrinho Ayroza , Marques Marcelo Rocha , Rossa Junior Carlos , Salo Tuula , Coletta Ricardo D.
Published: January 2023

Objective

Although there have been remarkable achievements in the molecular landscape of oral squamous cell carcinoma (OSCC) in recent years, bringing advances in the understanding of its pathogenesis, development and progression, little has been applied in the prognosis and choosing the optimal treatment. In this study, we explored the influence of the stress induced phosphoprotein 1 (STIP1), which is frequently reported to be highly expressed in many cancers, in OSCCs.

Methods

STIP1 expression was assessed in the TCGA database and in two independent cohorts by immunohistochemistry. Knockdown strategy was applied in OSCC cell lines to determine the impact of STIP1 on viability, proliferation, migration and invasion. The zebrafish model was applied for studying tumor formation and metastasis in vivo. The association of STIP1 and miR-218-5p was explored by bioinformatics and mimics transfection.

Results

STIP1 was highly expressed in OSCCs and significantly associated with shortened survival and higher risk of recurrence. STIP1 down-regulation decreased proliferation, migration and invasion of tumor cells, and reduced the number of metastases in the Zebrafish model. STIP1 and miR-218-5p were inversely expressed, and the transfection of miR-218-5p mimics into OSCC cells decreased STIP1 levels as well as proliferation, migration and invasion.

Conclusion

Our findings show that STIP1 overexpression, which is inversely associated with miR-218-5p levels, contributes to OSCC aggressiveness by controlling proliferation, migration and invasion and is a determinant of poor prognosis.
Endoscopic 1½-transseptal approach for pituitary surgery
Authors: Cong Zixiang , Zhu Junhao , Sun Huaiyu , Tang Chao , Yang Jin , Ma Chiyuan
Published: January 2023

Background

We previously introduced the one-and-a-half (1½) nostril endoscopic transsphenoidal approach (OETA) to reduce the damage to the nasal structures. Here, we reported the modified approach which is called the endoscopic 1½-transseptal approach (EOTA) for pituitary surgery by combining the OETA and the microscopic transseptal approach to simplify intranasal procedures and protect nasal mucosa. In EOTA, we removed the sellar lesions in a corridor that is composed of the right submucosal space and the anterior left ½ nasal cavity.

Methods

We introduced EOTA with a detailed technical description and preliminary clinical outcomes. A total of 128 patients who underwent EOTA for pituitary surgery from July 2018 to September 2020 were reviewed for evaluation of the safety and efficacy of this approach.

Results

EOTA had a high gross total resection (GTR) rate and a 1ow complication rate. GTR was achieved in 106 (82.8%) patients, with 81.4% for pituitary adenomas and 93.3% for other non-adenomatous lesions. Post-operative complications included 3 patients (2.3%) with postoperative cerebrospinal fluid leak, 3 patients (2.3%) with diabetes insipidus, 5 patients (3.9%) with anterior pituitary insufficiency and 2 patients (1.6%) with meningitis. In addition, EOTA simplified the intranasal procedures, which led to shortened operation time (67.8 minutes). The results of ASK nasal-12, the Lund-Kennedy score, and the odor identification test showed that patients who underwent EOTA recovered quickly after surgery and the nasal cavity returned to the preoperative condition both apparently and physiologically one month after surgery.

Conclusions

EOTA is a simple, safe and effective approach for pituitary lesions, which provides not only a sufficient surgical corridor for 2-surgeon/4- or 3-hands technique but also minimally invasive access to the sellar region.
Protocol for a multicenter, double-blind, randomized, placebo-controlled phase III trial of the inhaled β2-adrenergic receptor agonist salbutamol for transient tachypnea of the newborn (the REFSAL trial)
Authors: Madajczak Dariusz , Daboval Thierry , Lauterbach Ryszard , Łoniewska Beata , Błaż Witold , Szczapa Tomasz , Sadowska-Krawczenko Iwona , Michalak-Kloc Marzena , Sławska Helena , Borszewska-Kornacka Maria , Bokiniec Renata ,   the REFSAL Study Group
Published: January 2023

Background

Transient tachypnea of the newborn (TTN), which results from inadequate absorption of fetal lung fluid, is the most common cause of neonatal respiratory distress. Stimulation of β-adrenergic receptors enhances alveolar fluid absorption. Therefore, the β2-adrenergic receptor agonist salbutamol has been proposed as a treatment for TTN. This study aims to evaluate the efficacy and safety of salbutamol as supportive pharmacotherapy together with non-invasive nasal continuous positive airway pressure (NIV/nCPAP) for the prevention of persistent pulmonary hypertension of the newborn (PPHN) in infants with TTN.

Methods and analysis

This multicenter, double-blind, phase III trial will include infants with a gestational age between 32 and 42 weeks who are affected by respiratory disorders and treated in eight neonatal intensive care units in Poland. A total of 608 infants within 24 h after birth will be enrolled and randomly assigned (1:1) to receive nebulized salbutamol with NIV or placebo (nebulized 0.9% NaCl) with NIV. The primary outcome is the percentage of infants with TTN who develop PPHN. The secondary outcomes are the severity of respiratory distress (assessed with the modified TTN Silverman score), frequency of need for intubation, duration of NIV and hospitalization, acid–base balance (blood pH, partial pressure of O2 and CO2, and base excess), and blood serum ionogram for Na+, K+, and Ca2+.

Discussion

The Respiratory Failure with Salbutamol (REFSAL) study will be the first clinical trial to evaluate the efficacy and safety of salbutamol in the prevention of persistent pulmonary hypertension in newborns with tachypnea, and will improve short term outcomes. If successful, the study will demonstrate the feasibility of early intervention with NIV/nCPAP together with nebulized salbutamol in the management of TTN.

Ethics and dissemination

The study protocol was approved by the Bioethics Committee of the Medical University of Warsaw, Warsaw, Poland on November 16, 2020 (decision number KB/190/2020). All procedures will follow the principles of the Declaration of Helsinki. The results of the study will be submitted for knowledge translation in peer-reviewed journals and presented at national and international pediatric society conferences.

Clinical Trial Registration

It is registered at ClinicalTrials.gov NCT05527704, EudraCT 2020-003913-36; Protocol version 5.0 from 04/01/2022.
Intercostal nerve cryoanalgesia in the treatment of pain in patients operated on by the modified Nuss method with the BackOnFeet application—a new strategy to improve outcomes
Authors: Zacha Sławomir , Andrzejewska Agata , Jastrzębska-Ligocka Barbara , Szwed Aleksander , Modrzejewska Elżbieta , Zacha Wojciech , Skonieczna-Żydecka Karolina , Miegoń Jakub , Jarosz Konrad , Biernawska Jowita
Published: January 2023

GRAPHICAL ABSTRACT

Standarized care with ERAS protocol for NUSS surgery and its benefits.
Proteomics and weighted gene correlated network analysis reveal glutamatergic synapse signaling in diazepam treatment of alcohol withdrawal
Authors: Kong Wan , Huang Shanqing , Chen Zikai , Li Xiaolin , Liu Shujing , Zhang Zi , Yang Ye , Wang Zhanzhang , Zhu Xiuqing , Ni Xiaojia , Lu Haoyang , Zhang Ming , Li Zezhi , Wen Yuguan , Shang Dewei
Published: January 2023

Background: Alcohol use disorder (AUD) is characterized by chronic excessive alcohol consumption, often alternating with periods of abstinence known as alcohol withdrawal syndrome (AWS). Diazepam is the preferred benzodiazepine for treatment of alcohol withdrawal syndrome under most circumstances, but the specific mechanism underlying the treatment needs further research.

Methods: We constructed an animal model of two-bottle choices and chronic intermittent ethanol exposure. LC-MS/MS proteomic analysis based on the label-free and intensity-based quantification approach was used to detect the protein profile of the whole brain. Weighted gene correlated network analysis was applied for scale-free network topology analysis. We established a protein–protein interaction network based on the Search Tool for the Retrieval of Interacting Genes (STRING) database and Cytoscape software and identified hub proteins by CytoHubba and MCODE plugins of Cytoscape. The online tool Targetscan identified miRNA–mRNA pair interactions.

Results: Seven hub proteins (Dlg3, Dlg4, Shank3, Grin2b, Camk2b, Camk2a and Syngap1) were implicated in alcohol withdrawal syndrome or diazepam treatment. In enrichment analysis, glutamatergic synapses were considered the most important pathway related to alcohol use disorder. Decreased glutamatergic synapses were observed in the late stage of withdrawal, as a protective mechanism that attenuated withdrawal-induced excitotoxicity. Diazepam treatment during withdrawal increased glutamatergic synapses, alleviating withdrawal-induced synapse inhibition.

Conclusion: Glutamatergic synapses are considered the most important pathway related to alcohol use disorder that may be a potential molecular target for new interventional strategies.
Image encryption scheme based on a controlled zigzag transform and bit-level encryption under the quantum walk
Authors: Zhang Tian , Wang Shumei
Published: January 2023

With the rapid development of science and technology and network technology, the study of information security has become a hot spot, and image encryption has potential value in this regard. In this paper, an image encryption scheme based on controlled zigzag transform and bit-level encryption under the quantum walk environment is proposed. First, the parameters of the alternating quantum walk are obtained using the SHA-256 method, and the probability matrix of the quantum distribution on the two-dimensional lattice is obtained by multiple walk measurements; second, the spatial dislocation and bit-level dislocation of the image are realized by performing controlled zigzag dislocation and three-dimensional tesseract-like rotational dislocation on the color image; finally, after preprocessing the probability matrix of the quantum distribution, the matrix is bitwise with the dislocated image to achieve the encryption protection of image information. The effectiveness of the encryption scheme is verified by simulation experiments, and the scheme has a significant encryption effect. Compared with other encryption schemes, this scheme has better key sensitivity and dislocation effect, which provides a new approach to the field of image quantum encryption.
An aperture of 21 cm telescope with polarized coaxial for satellite laser ranging
Authors: Long Mingliang , Deng Huarong , Zhang Haifeng , Tan Kai , Lin Hai sheng , Wu Zhibo , Zhang Zhongping
Published: January 2023

With the development of aerospace and space scientific research, satellite laser ranging (SLR) has put forward higher requirements for response speed, data density, and measurement accuracy. In coaxial common optical path laser ranging, the emitted laser and the received laser echoes pass through the same optical system. Due to the reversibility of the optical path, the laser emission, monitoring, and laser echoes’ optical path all pass through the same optical system structure, and the response speed and ranging ability of the laser ranging system have been greatly improved. Based on the SLR system of the Shanghai Astronomical Observatory (SHAO), the laser transmitting telescope with an aperture of 21 cm was used to build a polarized coaxial SLR system. It uses a picosecond pulsed laser with a pulse repetition frequency of 2 kHz and a single-pulse energy of 2 mJ. Also, a 4f system was applied to shrink the laser echo beam and filter out noise, the measurements of low-Earth orbit and long-distance high-orbit satellites were realized, and the ranging accuracy was ∼2 cm. As far as we know, this is currently the smallest aperture telescope for SLR globally, which is conducive to the miniaturization and integrated development of SLR systems.
Athlete level, sport-type, and gender influences on training, mental health, and sleep during the early COVID-19 lockdown in Malaysia
Authors: Washif Jad Adrian , Kok Lian-Yee , James Carl , Beaven Christopher Martyn , Farooq Abdulaziz , Pyne David B. , Chamari Karim
Published: January 2023

Purpose: We evaluated the extent of changes in training practices, recovery, mental health, and sleep patterns of athletes during the early COVID-19 lockdown in a single country-cohort.

Methods: A total of 686 athletes (59% male, 41% female; 9% World Class, 28% International, 29% National, 26% State, 8% Recreational) from 50 sports (45% individual, 55% team) in Malaysia completed an online, survey-based questionnaire study. The questions were related to training practices (including recovery and injury), mental health, and sleep patterns.

Results: Relative to pre-lockdown, training intensity (−34%), frequency (−20%, except World-Class), and duration (−24%–59%, especially International/World-Class) were compromised, by the mandated lockdown. During the lockdown, more space/access (69%) and equipment (69%) were available for cardiorespiratory training, than technical and strength; and these resources favoured World-Class athletes. Most athletes trained for general strength/health (88%) and muscular endurance (71%); and some used innovative/digital training tools (World-Class 48% vs. lower classification-levels ≤34%). More World-Class, International, and National athletes performed strength training, plyometrics, and sport-specific technical skills with proper equipment, than State/Recreational athletes. More females (42%) sourced training materials from social media than males (29%). Some athletes (38%) performed injury prevention exercises; 18% had mild injuries (knees 29%, ankles 26%), and 18% received a medical diagnosis (International 31%). Lower-level athletes (e.g., State 44%) disclosed that they were mentally more vulnerable; and felt more anxious (36% vs. higher-levels 14%–21%). Sleep quality and quantity were “normal” (49% for both), “improved” (35% and 27%), and only 16% and 14% (respectively) stated “worsened” sleep.

Conclusion: Lockdown compromised training-related practices, especially in lower-level athletes. Athletes are in need of assistance with training, and tools to cope with anxiety that should be tailored to individual country requirements during lockdown situations. In particular, goal-driven (even if it is at home) fitness training, psychological, financial, and lifestyle support can be provided to reduce the difficulties associated with lockdowns. Policies and guidelines that facilitate athletes (of all levels) to train regularly during the lockdown should be developed.
Metabolic dysregulation and decreased capillarization in skeletal muscles of male adolescent offspring rats exposed to gestational intermittent hypoxia
Authors: Wongkitikamjorn Wirongrong , Wada Eiji , Hosomichi Jun , Maeda Hideyuki , Satrawaha Sirichom , Hong Haixin , Yoshida Ken-ichi , Ono Takashi , Hayashi Yukiko K.
Published: January 2023

Gestational intermittent hypoxia (IH) is a hallmark of obstructive sleep apnea that occurs frequently during pregnancy, and effects caused by this environmental change during pregnancy may be transmitted to the offspring. In this study, we aimed to clarify the effects of IH in pregnant rats on the skeletal muscle of adolescent offspring rats. Mother rats underwent IH from gestation day 7–21, and their 5-weeks-old male offspring were analyzed. All male offspring rats were born and raised under normoxia conditions. Although no general growth retardation was observed, we found that exposure to gestational IH reduces endurance running capacity of adolescent offspring rats. Both a respiratory muscle (diaphragm; DIA) and a limb muscle (tibialis anterior; TA) showed no histological abnormalities, including fiber size and fiber type distribution. To identify the possible mechanism underlying the reduced running capacity, regulatory factors associated with energy metabolism were analyzed in different parts of skeletal muscles. Compared with rats born under conditions of gestational normoxia, gestational IH offspring rats showed significantly lower expression of genes associated with glucose and lipid metabolism, and lower protein levels of phosphorylated AMPK and AKT. Furthermore, gene expression of adiponectin receptors one and two was significantly decreased in the DIA and TA muscles. In addition, the DIA muscle from adolescent rats had significantly decreased capillary density as a result of gestational IH. However, these changes were not observed in a sucking muscle (geniohyoid) and a masticating muscle (masseter) of these rats. These results suggest that respiratory and limb muscles are vulnerable to gestational IH, which induces altered energy metabolism with decreased aerobic motor function. These changes were partially owing to the decreased expression of adiponectin receptors and decreased capillary density in adolescent offspring rats.
DenseNet weed recognition model combining local variance preprocessing and attention mechanism
Authors: Mu Ye , Ni Ruiwen , Fu Lili , Luo Tianye , Feng Ruilong , Li Ji , Pan Haohong , Wang Yingkai , Sun Yu , Gong He , Guo Ying , Hu Tianli , Bao Yu , Li Shijun
Published: January 2023

Introduction

The purpose of this paper is to effectively and accurately identify weed species in crop fields in complex environments. There are many kinds of weeds in the detection area, which are densely distributed.

Methods

The paper proposes the use of local variance pre-processing method for background segmentation and data enhancement, which effectively removes the complex background and redundant information from the data, and prevents the experiment from overfitting, which can improve the accuracy rate significantly. Then, based on the optimization improvement of DenseNet network, Efficient Channel Attention (ECA) mechanism is introduced after the convolutional layer to increase the weight of important features, strengthen the weed features and suppress the background features.

Results

Using the processed images to train the model, the accuracy rate reaches 97.98%, which is a great improvement, and the comprehensive performance is higher than that of DenseNet, VGGNet-16, VGGNet-19, ResNet-50, DANet, DNANet, and U-Net models.

Discussion

The experimental data show that the model and method we designed are well suited to solve the problem of accurate identification of crop and weed species in complex environments, laying a solid technical foundation for the development of intelligent weeding robots.
High-resolution quantitative trait locus mapping for rice grain quality traits using genotyping by sequencing
Authors: Jin Su-Kui , Xu Li-Na , Yang Qing-Qing , Zhang Ming-Qiu , Wang Shui-Lian , Wang Ruo-An , Tao Tao , Hong Lian-Min , Guo Qian-Qian , Jia Shu-Wen , Song Tao , Leng Yu-Jia , Cai Xiu-Ling , Gao Ji-Ping
Published: January 2023

Rice is a major food crop that sustains approximately half of the world population. Recent worldwide improvements in the standard of living have increased the demand for high-quality rice. Accurate identification of quantitative trait loci (QTLs) for rice grain quality traits will facilitate rice quality breeding and improvement. In the present study, we performed high-resolution QTL mapping for rice grain quality traits using a genotyping-by-sequencing approach. An F2 population derived from a cross between an elite japonica variety, Koshihikari, and an indica variety, Nona Bokra, was used to construct a high-density genetic map. A total of 3,830 single nucleotide polymorphism markers were mapped to 12 linkage groups spanning a total length of 2,456.4 cM, with an average genetic distance of 0.82 cM. Seven grain quality traits—the percentage of whole grain, percentage of head rice, percentage of area of head rice, transparency, percentage of chalky rice, percentage of chalkiness area, and degree of chalkiness—of the F2 population were investigated. In total, 15 QTLs with logarithm of the odds (LOD) scores >4 were identified, which mapped to chromosomes 6, 7, and 9. These loci include four QTLs for transparency, four for percentage of chalky rice, four for percentage of chalkiness area, and three for degree of chalkiness, accounting for 0.01%–61.64% of the total phenotypic variation. Of these QTLs, only one overlapped with previously reported QTLs, and the others were novel. By comparing the major QTL regions in the rice genome, several key candidate genes reported to play crucial roles in grain quality traits were identified. These findings will expedite the fine mapping of these QTLs and QTL pyramiding, which will facilitate the genetic improvement of rice grain quality.
Acyl-CoA-dependent and acyl-CoA-independent avocado acyltransferases positively influence oleic acid content in nonseed triacylglycerols
Authors: Behera Jyoti , Rahman Md Mahbubur , Shockey Jay , Kilaru Aruna
Published: January 2023

In higher plants, acyl-CoA:diacylglycerol acyltransferase (DGAT) and phospholipid:diacylglycerol acyltransferase (PDAT) catalyze the terminal step of triacylglycerol (TAG) synthesis in acyl-CoA-dependent and -independent pathways, respectively. Avocado (Persea americana) mesocarp, a nonseed tissue, accumulates significant amounts of TAG (~70% by dry weight) that is rich in heart-healthy oleic acid (18:1). The oil accumulation stages of avocado mesocarp development coincide with high expression levels for type-1 DGAT (DGAT1) and PDAT1, although type-2 DGAT (DGAT2) expression remains low. The strong preference for oleic acid demonstrated by the avocado mesocarp TAG biosynthetic machinery represents lucrative biotechnological opportunities, yet functional characterization of these three acyltransferases has not been explored to date. We expressed avocado PaDGAT1, PaDGAT2, and PaPDAT1 in bakers’ yeast and leaves of Nicotiana benthamiana. PaDGAT1 complemented the TAG biosynthesis deficiency in the quadruple mutant yeast strain H1246, and substantially elevated total cellular lipid content. In vitro enzyme assays showed that PaDGAT1 prefers oleic acid compared to palmitic acid (16:0). Both PaDGAT1 and PaPDAT1 increased the lipid content and elevated oleic acid levels when expressed independently or together, transiently in N. benthamiana leaves. These results indicate that PaDGAT1 and PaPDAT1 prefer oleate-containing substrates, and their coordinated expression likely contributes to sustained TAG synthesis that is enriched in oleic acid. This study establishes a knowledge base for future metabolic engineering studies focused on exploitation of the biochemical properties of PaDGAT1 and PaPDAT1.
Innovative microbial disease biocontrol strategies mediated by quorum quenching and their multifaceted applications: A review
Authors: Zhu Xixian , Chen Wen-Juan , Bhatt Kalpana , Zhou Zhe , Huang Yaohua , Zhang Lian-Hui , Chen Shaohua , Wang Junxia
Published: January 2023

With the increasing resistance exhibited by undesirable bacteria to traditional antibiotics, the need to discover alternative (or, at least, supplementary) treatments to combat chemically resistant bacteria is becoming urgent. Quorum sensing (QS) refers to a novel bacterial communication system for monitoring cell density and regulation of a network of gene expression that is mediated by a group of signaling molecules called autoinducers (AIs). QS-regulated multicellular behaviors include biofilm formation, horizontal gene transfer, and antibiotic synthesis, which are demonstrating increasing pathogenicity to plants and aquacultural animals as well as contamination of wastewater treatment devices. To inhibit QS-regulated microbial behaviors, the strategy of quorum quenching (QQ) has been developed. Different quorum quenchers interfere with QS through different mechanisms, such as competitively inhibiting AI perception (e.g., by QS inhibitors) and AI degradation (e.g., by QQ enzymes). In this review, we first introduce different signaling molecules, including diffusible signal factor (DSF) and acyl homoserine lactones (AHLs) for Gram-negative bacteria, AIPs for Gram-positive bacteria, and AI-2 for interspecies communication, thus demonstrating the mode of action of the QS system. We next exemplify the QQ mechanisms of various quorum quenchers, such as chemical QS inhibitors, and the physical/enzymatic degradation of QS signals. We devote special attention to AHL-degrading enzymes, which are categorized in detail according to their diverse catalytic mechanisms and enzymatic properties. In the final part, the applications and advantages of quorum quenchers (especially QQ enzymes and bacteria) are summarized in the context of agricultural/aquacultural pathogen biocontrol, membrane bioreactors for wastewater treatment, and the attenuation of human pathogenic bacteria. Taken together, we present the state-of-the-art in research considering QS and QQ, providing theoretical evidence and support for wider application of this promising environmentally friendly biocontrol strategy.
Characterizing stomatal attributes and photosynthetic induction in relation to biochemical changes in Coriandrum sativum L. by foliar-applied zinc oxide nanoparticles under drought conditions
Authors: Ahmed Shakil , Khan Muhammad Tajammal , Abbasi Asim , Haq Inzamam Ul , Hina Aiman , Mohiuddin Muhammad , Tariq Muhammad Atiq Ur Rehman , Afzal Muhammad Zaheer , Zaman Qamar uz , Ng Anne Wai Man , Li Yong
Published: January 2023

Abiotic stress, particularly drought, will remain an alarming challenge for sustainable agriculture. New approaches have been opted, such as nanoparticles (NPs), to reduce the negative impact of drought stress and lessen the use of synthetic fertilizers and pesticides that are an inevitable problem these days. The application of zinc oxide nanoparticles (ZnO NPs) has been recognized as an effective strategy to enhance plant growth and crop production during abiotic stress. The aim of the current study was to investigate the role of ZnO NPs in drought stress management of drought-susceptibleCoriandrum sativumL. (C. sativum) in two consecutive seasons. Drought regimes (moderate drought regime—MDR and intensive drought regime—IDR) were developed based on replenishment method with respect to 50% field capacity of fully irrigated (control) plants. The results showed that foliar application of 100 ppm ZnO NPs improved the net photosynthesis (Pn), stomatal conductance (C), and transpiration rate (E) and boosted up the photosynthetic capacity associated with photosynthetic active radiation in MDR. Similarly, 48% to 30% improvement of chlorophyll b content was observed in MDR and onefold to 41% in IDR during both seasons in ZnO NP-supplemented plants. The amount of abscisic acid in leaves showed a decreasing trend in MDR and IDR in the first season (40% and 30%) and the second season (49% and 33%) compared with untreated ZnO NP plants. The ZnO NP-treated plants showed an increment in total soluble sugars, total phenolic content, and total flavonoid content in both drought regimes, whereas the abaxial surface showed high stomatal density and stomatal index than the adaxial surface in foliar-supplied NP plants. Furthermore, ZnO NPs improve the magnitude of stomata ultrastructures like stomatal length, stomatal width, and pore length for better adaptation against drought. Principal component analysis revealed the efficacy of ZnO NPs in inducing drought tolerance in moderate and intensive stress regimes. These results suggest that 100 ppm ZnO NPs can be used to ameliorate drought tolerance inC. sativumplants.
Large investment of stored nitrogen and phosphorus in female cones is consistent with infrequent reproduction events of Pinus koraiensis, a high value woody oil crop in Northeast Asia
Authors: Wu Haibo , Zhang Jianying , Rodríguez-Calcerrada Jesús , Salomón Roberto L. , Yin Dongsheng , Zhang Peng , Shen Hailong
Published: January 2023

Pinus koraiensis is famous for its high-quality timber production all the way and is much more famous for its high value health-care nut oil production potential since 1990’s, but the less understanding of its reproduction biology seriously hindered its nut productivity increase. Exploring the effects of reproduction on nutrient uptake, allocation and storage help to understand and modify reproduction patterns in masting species and high nut yield cultivar selection and breeding. Here, we compared seasonality in growth and in nitrogen ([N]) and phosphorus ([P]) concentrations in needles, branches and cones of reproductive (cone-bearing) and vegetative branches (having no cones) of P. koraiensis during a masting year. The growth of one- and two-year-old reproductive branches was significantly higher than that of vegetative branches. Needle, phloem and xylem [N] and [P] were lower in reproductive branches than in vegetative branches, although the extent and significance of the differences between branch types varied across dates. [N] and [P] in most tissues were high in spring, decreased during summer, and then recovered by the end of the growing season. Overall, [N] and [P] were highest in needles, lowest in the xylem and intermediate in the phloem. More than half of the N (73.5%) and P (51.6%) content in reproductive branches were allocated to cones. There was a positive correlation between cone number and N and P content in needles (R2 = 0.64, R2 = 0.73) and twigs (R2 = 0.65, R2 = 0.62) of two-year-old reproductive branches. High nutrient sink strength of cones and vegetative tissues of reproductive branches suggested that customized fertilization practices can help improve crop yield in Pinus koraiensis.
Genome-wide analysis of epigenetic and transcriptional changes in the pathogenesis of RGSV in rice
Authors: Wu Xiaoqing , Liu Hongfei , Lian Bi , Jiang Xue , Chen Cheng , Tang Tianxin , Ding Xinlun , Hu Jie , Zhao Shanshan , Zhang Shuai , Wu Jianguo
Published: January 2023

Rice grassy stunt virus (RGSV), a typical negative single-stranded RNA virus, invades rice and generates several disease signs, including dwarfing, tillering, and sterility. Previous research has revealed that RGSV-encoded proteins can force the host’s ubiquitin-proteasome system to utilize them for viral pathogenesis. However, most of the studies were limited to a single omics level and lacked multidimensional data collection and correlation analysis on the mechanisms of RGSV-rice interactions. Here, we performed a comprehensive association analysis of genome-wide methylation sequencing, transcriptome sequencing, and histone H3K9me3 modification in RGSV-infested as well as non-infested rice leaves, and the levels of all three cytosine contexts (CG, CHG and CHH) were found to be slightly lower in RGSV-infected rice leaves than in normal rice. Large proportions of DMRs were distributed in the promoter and intergenic regions, and most DMRs were enriched in the CHH context, where the number of CHH hypo-DMRs was almost twice as high as that of hyper-DMRs. Among the genes with down-regulated expression and hypermethylation, we analyzed and identified 11 transcripts involved in fertility, plant height and tillering, and among the transcribed up-regulated and hypermethylated genes, we excavated 7 transcripts related to fertility, plant height and tillering. By analyzing the changes of histone H3K9me3 modification before and after virus infestation, we found that the distribution of H3K9me3 modification in the whole rice genome was prevalent, mainly concentrated in the gene promoter and gene body regions, which was distinctly different from the characteristics of animals. Combined with transcriptomic data, H3K9me3 mark was found to favor targeting highly expressed genes. After RGSV infection, H3K9me3 modifications in several regions of CTK and BR hormone signaling-related genes were altered, providing important targets for subsequent studies.
Fruit shape loci sun, ovate, fs8.1 and their interactions affect seed size and shape in tomato
Authors: Chen Jie , Pan Bingqing , Li Zixiong , Xu Yue , Cao Xiaomeng , Jia Jingjing , Shen Huolin , Sun Liang
Published: January 2023

Seed size and shape are not only critical for plant reproduction and dispersal, but also important agronomic traits. Tomato fruit shape loci sun, ovate and fs8.1 regulate the morphology of fruit, flower, leaf and stem, and recently their functions in seed morphogenesis have also been noticed. However, mechanism underlying seed morphology variation has not been systematically investigated yet. Thus, using the near isogenic lines (NILs) harboring one, two or three of the fruit shape loci, histological, physiological and transcriptional bases of seed morphology change have been studied. sun and ovate showed potential abilities in decreasing seed size, whereas, fs8.1 had a potential ability in increasing this parameter. Interactions between two loci and the interaction among three loci all led to significant decrease of seed size. All the loci significantly down-regulated seed shape index (SSI), except for sun/fs8.1 double NIL, which resulted in the reductions in both seed length and width and finally led to a decreased trend of SSI. Histologically, seed morphological changes were mainly attributed to the cell number variations. Transcriptional and physiological analyses discovered that phytohormone-, cytoskeleton- as well as sugar transportation- and degradation-related genes were involved in the regulation of seed morphology by the fruit shape loci.
Effects of grazing on vegetation diversity and soil multifunctionality in coconut plantations
Authors: Duan Qianwen , Hu An , Yang Weibo , Yu Ruoyun , Liu Guodao , Huan Hengfu , Dong Rongshu , Li Xinyong
Published: January 2023

Grazing is the main way of utilizing understory vegetation in the tropics. However, the effects of grazing on vegetation diversity and soil functions in coconut plantations remain unclear. Therefore, this study was conducted in a young coconut plantation that was grazed by geese in Wenchang, China. We identified four grazing intensities according to the aboveground biomass, namely, no grazing (CK), light grazing (LG), moderate grazing (MG), and heavy grazing (HG). In April 2022, we used the quadrat method to investigate the composition and traits of vegetation, collected and analyzed 0–40-cm soil samples in each grazing intensity. The results showed that grazing changed the composition of understory species. The predominant species changed from Bidens pilosa to Praxelis clematidea + Paspalum thunbergii and then to P. clematidea with increasing grazing intensity. The richness, Shannon-Wiener index, evenness, modified functional attribute diversity (MFAD), functional divergence (Fdiv), and functional evenness (Feve) of CK were 4.5, 1.0, 0.29, 0.20, 0.84, and 0.80, respectively. Taxonomic diversity did not respond to LG, but responded significantly to MG and HG. Compared with CK, MG and HG increased richness by 96% and 200%, respectively, and Shannon-Wiener index increased by 40% and 98%, respectively. HG increased evenness by 95%. For functional diversity, MG and HG increased MFAD by 164% and 560%, respectively, but Fdiv and Feve did not respond to grazing intensity. The carbon (C) functioning, nitrogen (N) functioning, phosphorus (P) functioning, and multifunctionality in the 0–10-cm topsoil of CK were −0.03, 0.37, −0.06, 0.20, and 0.14, respectively. Grazing increased C functioning, P functioning, and multifunctionality in the 0–10-cm topsoil but decreased N functioning. Multiple linear regression showed that the taxonomic diversity and functional diversity could be used to estimate soil functions, but these vary among soil layers. In general, MG and HG can increase vegetation diversity and soil function. It may be possible to promote even distribution of geese by adding water sources or zoning grazing. Furthermore, quantitative grazing experiments are needed to determine the efficient use pattern of the understory in coconut plantations in tropics.
Metabolic profiling, in-situ spatial distribution, and biosynthetic pathway of functional metabolites in Dendrobium nobile stem revealed by combining UPLC-QTOF-MS with MALDI-TOF-MSI
Authors: Liu Qingling , Huang Yuan , Linghu Chu , Xiao Jianfen , Gu Ronghui
Published: January 2023

The stem of Dendrobium nobile Lindl. (Orchidaceae), called “Shihu” in traditional Chinese medicine, is a well-known medicinal and edible plant material in China. It is used as an antipyretic, analgesic, and tonic to nourish the stomach and Yin (i.e., to improve the production of body fluids). These therapeutic properties are attributed to its alkaloids, sesquiterpenoids, bibenzyls, fluorenones, and phenanthrenes. However, a comprehensive understanding of these metabolites and their spatial distribution in stems is lacking. In this study, ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was performed to obtain detailed metabolites information about D. nobile stems. Then, the spatial distributions of diverse metabolites, including alkaloids and sesquiterpenoids, were characterized and visualized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF-MSI). Based on the spatial and metabolic profiling data, sesquiterpene alkaloid dendrobine was chosen for the exhaustive study of a biosynthetic pathway in D. nobile. This is the first report on mass spectrometry imaging for Dendrobium species. As a result, critical bioactive metabolites such as 11 alkaloids, 10 sesquiterpenes, and 13 other metabolites were putatively identified and relatively quantified. The identified alkaloids were distributed in the parenchyma or vascular bundle, and sesquiterpenes were present in all regions of the stem with higher abundance in the vascular bundle and cuticle, or in the cuticle and epidermis. The biosynthetic pathway and accumulation pattern of dendrobine in D. nobile stem were also proposed. Our findings not only provided a critical methodology for the thorough understanding of physiological changes in metabolites and precise utilization of D. nobile stem, but also displayed an effective strategy for insight into the biosynthesis of bioactive metabolites in plants.
Reliability of attention bias and attention bias variability to climate change images in the dot-probe task
Authors: Carlson Joshua M. , Fang Lin , Coughtry-Carpenter Caleb , Foley John
Published: January 2023

Climate change is one of the most pressing issues of the 21st century, which is perhaps why information about climate change has been found to capture observers’ attention. One of the most common ways of assessing individual differences in attentional processing of climate change information is through the use of reaction time difference scores. However, reaction time-based difference scores have come under scrutiny for their low reliability. Given that a primary goal of the field is to link individual differences in attention processing to participant variables (e.g., environmental attitudes), we assessed the reliability of reaction time-based measures of attention processing of climate change information utilizing an existing dataset with three variations of the dot-probe task. Across all three samples, difference score-based measures of attentional bias were generally uncorrelated across task blocks (r = −0.25 to 0.31). We also assessed the reliability of newer attention bias variability measures that are thought to capture dynamic shifts in attention toward and away from salient information. Although these measures were initially found to be correlated across task blocks (r = 0.17–0.67), they also tended to be highly correlated with general reaction time variability (r = 0.49–0.83). When controlling for general reaction time variability, the correlations across task blocks for attention bias variability were much weaker and generally nonsignificant (r = −0.25 to 0.33). Furthermore, these measures were unrelated to pro-environmental disposition indicating poor predictive validity. In short, reaction time-based measures of attentional processing (including difference score and variability-based approaches) have unacceptably low levels of reliability and are therefore unsuitable for capturing individual differences in attentional bias to climate change information.
The influence of home environmental factors on kindergarten children’s addition strategy use
Authors: DePascale Mary , Jaeggi Susanne M. , Ramani Geetha B.
Published: January 2023

Young children vary widely in their levels of math knowledge, their abilities to solve math problems, and the strategies they use to solve math problems. As much of later math builds on children’s early understanding of basic math facts and problem-solving strategies, understanding influences on children’s early problem solving is important. Few studies, however, have examined the home environment in relation to children’s strategy use during arithmetic problems. We examined how both structural characteristics of children’s home environments, such as socioeconomic status (SES), as well as the learning environment, such as engagement in math and literacy activities at home, related to their use of problem-solving strategies for numerical addition problems. Kindergarten children from diverse backgrounds completed a measure of addition problem solving and strategy use, including simple and complex numerical problems. Strategies were coded based on a combination of accuracy and strategy sophistication, with higher scores indicating problems solved correctly with more sophisticated strategies. Parents completed a home activities questionnaire, reporting the frequency with which they and their child had engaged in math and literacy activities at home over the past month. An exploratory factor analysis identified three components of the home activities - a basic activities factor, an advanced math activities factor, and a literacy activities factor. Findings indicated that SES related to children’s strategy sophistication, and frequency of engaging in advanced math and literacy activities at home predicted strategy sophistication, however, engaging in activities at home did not moderate the relations between SES and strategy sophistication. This suggests that family engagement in activities at home may promote early arithmetic skills, and that the role of home environmental characteristics should be considered in children’s arithmetic strategy use and performance over development.
Psychiatric disorders and comorbidity in a Spanish sample of prisoners at the end of their sentence: Prevalence rates and associations with criminal history
Authors: Pagerols Mireia , Valero Sergi , Dueñas Lourdes , Bosch Rosa , Casas Miquel
Published: January 2023

Introduction

This study examined, for the first time, the prevalence of mental disorders and comorbidities among inmates who were about to be released, and their association with criminal history.

Methods

A Spanish sample of 140 prisoners at the end of their sentence was recruited from an occupational program. Psychiatric disorders were determined according to the Diagnostic and Statistical Manual of Mental Disorders criteria. Bivariate analyses followed by multivariate regression models were conducted to identify significant variables for repeat incarceration and violent offending.

Results

The lifetime prevalence of Axis I disorders was 81.4%, with substance use disorders (SUD) and attention deficit/hyperactivity disorder (ADHD) being the most common diagnoses (51.4 and 31.4%, respectively). The current prevalence of Axis I disorders was 59.0%, including learning disorders (38.6%), ADHD (16.4%), and SUD (5.71%) among the most frequent syndromes. Thirty-six (26.5%) participants met criteria for a current Axis II disorder, which commonly was an antisocial personality disorder (12.5%). The majority of the sample (60.8%) suffered from two or more comorbid disorders during their lifetime, although the current prevalence fell to 23.3%. Childhood ADHD increased the number of imprisonments, while inmates convicted of a violent crime were more likely to present a learning disorder. Having a lifetime diagnosis of SUD or multiple psychiatric disorders appeared to be associated with both repeat incarceration and violent offending.

Conclusion

Given the high rate of mental disorders still present among subjects completing prison sentences and the challenges they may encounter to benefit from vocational programs, our results suggest that appropriate psychiatric care should be provided during imprisonment and after release to facilitate their community reintegration.
When nerves hit: The effect of trait anxiety, situational stress, and task mastery on the perception and interpersonal accuracy of musical expressiveness
Authors: Chang-Arana Álvaro M. , Mavrolampados Anastasios , Pokki Niklas , Thompson Marc R.
Published: January 2023

Music performance anxiety (MPA) is a prevalent phenomenon with potentially serious consequences to a musician’s wellbeing and professional career. Yet, MPA does not always affect performance quality. It is hypothesized that trait anxiety, situational stress, and task mastery can exacerbate the effects of MPA and affect performance quality. Furthermore, it is unclear whether these effects are noticeable to both listeners and performing musicians. We measure performance quality as the expressiveness scores assigned by musicians and listeners to a set of pre-recorded performances. We selected three pianists with low, mid, and high MPA. Each pianist performed two pieces of their choice, familiar and unfamiliar, which were performed in rehearsal and recital conditions. The performances were videoed and edited into shorter clips for being presented to the performing pianists and to a set of online raters. Listeners and pianists will be asked to rate the expressiveness of all clips. We will determine the difference between the listeners’ perceived expressiveness and the pianists’ own expressiveness scores to estimate how well did listeners understand the pianists’ expressive intentions. We investigate (1) what is the effect of trait anxiety, situational stress, and task mastery on the listener’s perception of expressiveness and (2) what is the effect of these same variables on the listeners’ understanding of expressiveness.