bluuebunny/crossref_metadata_2025 huggingface dataset:
Deep decarbonization pathways in the building sector: China’s NDC and the Paris agreement
Authors: Xing Rui , Hanaoka Tatsuya , Masui Toshihiko
Published: January 2021

China’s economic growth has been largely relying on the consumption of coal. The country has realized that its economic development has to be free from dependence on fossil fuels. On 30 June 2015, China submitted its ‘Nationally Determined Contribution (NDC)’ in preparation for the Conference of Parties 21 (COP21). One of the important actions in China’s NDC is to lower carbon dioxide (CO2) emissions per unit of GDP by 60% to 65% from 2005 levels by 2030. This study examines the efforts from China’s building sector (i.e. urban residential, rural residential and service) in achieving the CO2 reduction target stated in China’s NDC. Furthermore, this study also explores the post-NDC era and looks into the opportunities towards deep decarbonization in the building sector by mid-century for contributing to the Paris agreement. The study covers 31 provincial regions of mainland China anddisparities of climate and socioeconomic indicators across regions are fully considered. We use a bottom-up cost optimization model called AIM/Enduse to evaluate the CO2 reduction potential brought by efficient technologies in China’s building sector. Five scenarios are designed to illustrate the emission pathways through 2050. The results show that, when energy constraint and emission target is introduced in mitigation scenarios, new generation biomass contribute a lot to emission reduction. Reduction potential in the nearly zero emission scenario is mainly from the urban residential sector, and to achieve deep decarbonization by 2050, it is important to bring a significant reduction of per-capita energy consumption in addition to ci improvement both in urban and rural households. Co-benefit analysis suggests that air pollutants can also be significantly reduced by deep decarbonization policies.
Local invariants of braiding quantum gates—associated link polynomials and entangling power
Authors: Padmanabhan Pramod , Sugino Fumihiko , Trancanelli Diego
Published: January 2021

For a generic n-qubit system, local invariants under the action of

S
L

(

2
,
C

)

⊗
n

characterize non-local properties of entanglement. In general, such properties are not immediately apparent and hard to construct. Here we consider two-qubit Yang–Baxter operators and show that their eigenvalues completely determine the non-local properties of the system. Moreover, we apply the Turaev procedure to these operators and obtain their associated link/knot polynomials. We also compute their entangling power and compare it with that of a generic two-qubit operator.
Biofabrication of spheroids fusion-based tumor models: computational simulation of glucose effects
Authors: Bustamante David J , Basile Elijah J , Hildreth Brady M , Browning Nathan W , Jensen S Alexander , Moldovan Leni , Petrache Horia I , Moldovan Nicanor I
Published: January 2021

In vitro tumor models consisting of cell spheroids are increasingly used for mechanistic studies and pharmacological testing. However, unless vascularized, the availability of nutrients such as glucose to deeper layers of multicellular aggregates is limited. In addition, recent developments in cells-only biofabrication (e.g. ‘scaffold-free bioprinting’), allow the creation of more complex spheroid-based structures, further exposing the cells to nutrient deprivation within these constructs. To explore the impact of glucose availability on such tumor-like structures, we used the CompuCell3D platform for modeling of tumor spheroids. By monitoring the types of cells, fusing pairs geometry and the distance between spheroids centers of mass, we made novel heuristic observations on how binary- and multi-spheroid fusions are impacted by glucose availability. At limiting glucose concentrations mimicking hypoglycemia we noted an abrupt collapse of the tumor spheroids, unexpectedly amplified by the contact with normal cell spheroids. At higher glucose concentrations, we found an increased intermixing of cancerous cells, strong anti-phase oscillations between proliferating and quiescent tumor cells and a structural instability of fusing tumor spheroids, leading to their re-fragmentation. In a model of tumor microenvironment composed of normal cell spheroids fusing around a tumoral one, the competition for glucose lead to either the tumor’s disappearance, to a steady state, or to its expansion. Moreover, the invasion of this microenvironment by individual tumor cells was also strongly depended on the available glucose. In conclusion, we demonstrate the value of computational simulations for anticipating the properties of biofabricated tumor models, and in generating testable hypotheses regarding the relationship between cancer, nutrition and diabetes.
Recent advancements towards large-scale flow diagnostics by robotic PIV
Authors: Scarano Fulvio , Jux Constantin , Sciacchitano Andrea
Published: January 2021

The present work reviews the recent developments in the domain of particle image velocimetry (PIV) with attention to its use for large-scale problems of interest for industrial aerodynamics. The article introduces the fundamental principles of flow seeding for large-scale experiments based on the helium-filled soap bubble (HFSB) technique. The measurement of flow tracers in a three-dimensional domain is presented, which is based on Lagrangian tracking methods. Furthermore, the concept of co-axial volumetric velocimetry is introduced and its combination with robotic arm manipulation is shown. The work presents two main examples of applications to aerodynamic problems: the first is the flow field around a propeller aircraft at wind tunnel free-stream velocity up to 50 m s−1, which to date is considered the boundary of large-scale PIV application with HFSB. The second example is the complex three-dimensional flow field around a cyclist at full scale. The work summarizes developments obtained in the last 3 years of research, demonstrating that the domain of PIV techniques is still undergoing vivid developments.
Positron impact excitation of lowest autoionizing state of potassium atom using distorted wave method with absorption and polarization potentials
Authors: William Noah Nzeki , Jobunga Eric Ouma , Singh Chandra Shekhar
Published: January 2021

Differential and integral cross-sections, as well as the alignment and lambda parameters for positron impact excitation of the lowest autoionizing state of potassium atom, have been calculated using a non-relativistic distorted wave method in the energy range of 18.9–1500 eV. The distortion potential in the initial and final channel state comprises a linear combination of static potential plus polarization and absorption potentials. The approximate Roothan-Hartree–Fock (RHF) wavefunctions Multi zeta wave functions given by Clementi and Roetti have been used. The inclusion of polarization and absorption potentials has improved integral cross-section and alignment parameter results. The integral cross sections have shown the near-threshold resonance structure revealed in electron impact excitation experimental results. Differential cross-section and lambda parameter results have been calculated and presented for future comparison since there are no other results available in the literature for positron impact excitation.
15% reduction in AC loss of a 3-phase 1 MVA HTS transformer by exploiting asymmetric conductor critical current
Authors: Jiang Zhenan , Song Wenjuan , Pei Xiaoze , Fang Jin , Badcock Rodney A , Wimbush Stuart C
Published: January 2021

An asymmetric dependence of the critical current on the direction of an applied magnetic field in HTS coated conductors has a non-trivial influence on the AC loss of coil windings. We report the modelled influence of real conductor critical current asymmetry on the AC loss characteristics of a 1 MVA HTS transformer design previously demonstrated by the Robinson Research Institute as well as a stand-alone coil having the same geometrical and electrical parameters as the low voltage (high current) winding of the transformer. We compare two commercial HTS conductors with distinctive differences in their critical current asymmetry and show a maximum variation of 15% and 29% in the calculated AC loss of the transformer and the stand-alone coil winding, respectively, when the conductor orientation is varied in the top and bottom halves of the windings. AC loss simulation giving consideration to asymmetric conductor critical current before winding the transformer could lead to substantial AC loss reduction even using the same amount of conductor and the same transformer design.
Home Blood Pressure Monitoring: Current Status and New Developments
Authors: Kario Kazuomi
Published: January 2021

Home blood pressure monitoring (HBPM) is a reliable, convenient, and less costly alternative to ambulatory blood pressure monitoring (ABPM) for the diagnosis and management of hypertension. Recognition and use of HBPM have dramatically increased over the last 20 years and current guidelines make strong recommendations for the use of both HBPM and ABPM in patients with hypertension. The accuracy and reliability of home blood pressure (BP) measurements require use of a validated device and standardized procedures, and good patient information and training. Key HBPM parameters include morning BP, evening BP, and the morning–evening difference. In addition, newer semi-automatic HBPM devices can also measure nighttime BP at fixed intervals during sleep. Advances in technology mean that HBPM devices could provide additional relevant data (e.g., environmental conditions) or determine BP in response to a specific trigger (e.g., hypoxia, increased heart rate). The value of HBPM is highlighted by a growing body of evidence showing that home BP is an important predictor of target organ damage, and cardiovascular disease (CVD)- and stroke-related morbidity and mortality, and provides better prognostic information than office BP. In addition, use of HBPM to monitor antihypertensive therapy can help to optimize reductions in BP, improve BP control, and reduce target organ damage and cardiovascular risk. Overall, HBPM should play a central role in the management of patients with hypertension, with the goal of identifying increased risk and predicting the onset of CVD events, allowing proactive interventions to reduce risk and eliminate adverse outcomes.
Physiologic Effects and Symptoms Associated with Extended-Use Medical Mask and N95 Respirators
Authors: Garra Gabrielle M , Parmentier Darlene , Garra Gregory
Published: January 2021

Objectives

Compliance and tolerance of facemasks for extended periods are legitimate concerns. The goal of this study was to identify the physiologic and symptomatic effects of extended-use N95 filtering facepiece respirator (N95) compared with medical masks. We hypothesized that hospital personnel wearing medical masks alone would report fewer subjective complaints compared with personnel wearing an N95 with or without overlying medical mask.

Methods

This was a nonrandomized cohort study of hospital-based healthcare personnel at a single tertiary center wearing a medical mask alone or N95 with or without overlying medical mask during routine clinical activity. Potential subjects were consented and asked to complete a structured survey, including a 10-point Likert scale for subjective symptoms: headache, lightheadedness, breathlessness, facial bruising, facial irritation, mental fatigue, physical fatigue, and yawning. Study investigators also obtained vital signs on the participants. Results between subjects wearing a medical mask and subjects wearing an N95 were compared. A sample of 144 subjects, 72 in each mask cohort, was needed to detect a 20% difference in a composite outcome of headache, shortness of breath, or lightheadedness between groups with an alpha of 0.05 and power of 0.8.

Results

We enrolled 72 subjects in each group. There were no differences in baseline demographics. Overall 77% of the cohort reported subjective symptoms while donning a mask. There was no difference in the composite outcome, no difference recorded symptoms except facial bruising, and no difference in physiologic measures between groups.

Conclusions

Most medical mask and N95 users reported symptoms during mask use. However, there was no difference in the symptom proportion or severity in either user.
Cultural Bias in Intelligence Assessment Using a Culture-Free Test in Moroccan Children
Authors: Lozano-Ruiz Alvaro , Fasfous Ahmed F , Ibanez-Casas Inmaculada , Cruz-Quintana Francisco , Perez-Garcia Miguel , Pérez-Marfil María Nieves
Published: January 2021

Objective

Previous research has shown that cognitive tests can lead to misclassification when applying non-representative norms to measure cognitive performance. The objective of this study was to investigate whether this misclassification also occurs with a non-verbal so-called “culture-free” intelligence test administered to different age groups.

Method

The intelligence of a sample of healthy Moroccan children (N = 147) ages 7, 9, and 11 was assessed using the Coloured Raven’s Progressive Matrices (CPM). Raw scores were used to study age differences, as well as misclassifications when applying the norms of three countries culturally different from Morocco (United Kingdom, Spain, and Oman).

Results

Intelligence performance was not within the normal range when non-representative norms were applied to the Moroccan raw scores. Misclassifications accounted for a large percentage of the participants that supposedly displayed intelligence deficits, especially when applying the British norms. Up to 15.68% of the healthy children fell within the “intellectually impaired” range, and up to 62.5% fell “below average,” with these percentages especially higher at older ages.

Conclusions

Our findings confirm that “culture-free” tests should be adapted to each culture and applied together with their culture’s specific norms to prevent misclassification and allow for a better, unbiased neuropsychological assessment.
What Is the Adequate PRP Dose for an Effective Treatment? An In Vitro Experimental Study on the Skin
Authors: Görgü Metin , Gökkaya Ali , Karanfil Ertuğrul
Published: January 2021

Background

The clinical results of many studies on platelet-rich plasma (PRP) differ due to a lack of standardization in PRP preparation and administration as well as many variables such as PRP preparation methods, platelet concentration, and platelet activation.

Objectives

The authors sought to investigate a different variable that will affect PRP application results. How much PRP should be injected into the unit area of tissue for an effective PRP treatment?

Methods

The study was performed on fresh surplus tissues of 20 patients that were discarded in abdominoplasty and mammoplasty operations. Nine areas of 4 cm2 were marked on the skin. Fluorescein-stained PRP was injected intradermally with 3 different gauge needles at 3 different doses (0.01, 0.03, and 0.05 mL). After injections, spreads of the fluorescent dye–covered areas in horizontal and vertical planes were measured and compared. For the horizontal plane measurements, the dye spread was measured first from the surface of the skin and second from the dermal surface of the skin. In addition, the width and depth of the dye spread in the dermis were measured from vertical sections.

Results

Changing the needle diameter does not affect the width or depth (thickness) of the PRP spread in the dermis. Increasing the applied dose to 0.03 mL increases the spread to the width and depth (thickness).

Conclusions

In research evaluating the effectiveness of PRP treatments, it is necessary to report the volume of PRP to be applied per unit of tissue.
Sexual selection on jumping spider color pattern: investigation with a new quantitative approach
Authors: Zhou Wei , Yu Long , Kwek Bernetta Z W , Jin Ge , Zeng Hua , Li Daiqin
Published: January 2021

How animals assess information encoded in individual color patches have been extensively studied, yet the role of both individual color patches and gross color pattern (i.e., the combination of color patches) remains understudied. We tested the functioning of both individual color patches and gross color pattern in sexual selection using the jumping spider Siler semiglaucus as a study system. We first quantified sexual dimorphism in S. semiglaucus in both individual patches and gross color pattern using the newly developed quantitative color pattern analysis (QCPA) framework. After detecting sexual differences in color coverage and pattern contrast, we manipulated the abdomen color pattern of males and had them engage in both female mate choice and male contest trials. Females spent more time watching males with lower pattern contrast and greater red coverage during mate assessment, suggesting that they evaluate information from both individual patches and gross color pattern of males. However, male color pattern had no significant effect on the outcomes of male contests. Thus, we suggest that the observed sexual color pattern dimorphism evolved primarily through female mate choice in S. semiglaucus. This is the first study to use QCPA framework to quantify sexual dimorphism in within-pattern conspicuousness from an intraspecific perspective in invertebrates. Our study also highlights the importance of both individual color patches and gross color pattern in sexual selection.
Density-dependent individual variation in male attractiveness in a wild field cricket
Authors: Niemelä Petri T , Tiso Stefano , Dingemanse Niels J
Published: January 2021

Social environments modify a male’s ability to attract females and thus affect its fitness. Theory implies that an individual’s fitness should trade-off with its ability to cope with competition. Individuals are expected to solve this trade-off differently: some males should be more attractive at low but others instead at high density. This prediction has rarely been tested in the wild. We used an automated RFID-surveillance system to quantify for each hour of the day, over 30 days (i.e., almost the entire adult lifespan of our model organism), whether a male had attracted a female in its burrow. The data were collected across a range of naturally varying local densities in wild field crickets, Gryllus campestris. We also estimated whether the shape of the relationship between attractiveness and density was under selection. At the population level, attractiveness increased from low to intermediate density, suggesting an Allee effect. Attractiveness subsequently declined at higher densities, for example, because of detrimental effects of increased competition. Opposite to expectations, males that were more attractive under low densities were also more attractive under higher densities. However, the increase in attractiveness with density varied among males, suggesting that Allee effects were individual-specific. Finally, selection was not acting on density-dependent attractiveness but males that lived longer acquired more mating partners. Our study reveals that social environments shape attractiveness in wild male insects, and imply the occurrence of individual-specific Allee effect that may be evolvable.
mzRecal: universal MS1 recalibration in mzML using identified peptides in mzIdentML as internal calibrants
Authors: Marissen Rob , Palmblad Magnus
Published: January 2021

In mass spectrometry-based proteomics, accurate peptide masses improve identifications, alignment and quantitation. Getting the most out of any instrument therefore requires proper calibration. Here, we present a new stand-alone software, mzRecal, for universal automatic recalibration of data from all common mass analyzers using standard open formats and based on physical principles.

Availability and implementation

mzRecal is implemented in Go and freely available on https://github.com/524D/mzRecal.

Supplementary information

Supplementary data are available at Bioinformatics online.
A national propensity score-matched analysis of emergency laparoscopic versus open abdominal surgery
Authors: Pucher P H , Mackenzie H , Tucker V , Mercer S J
Published: January 2021

Background

Laparoscopy has been widely adopted in elective abdominal surgery but is still sparsely used in emergency settings. The study investigated the effect of laparoscopic emergency surgery using a population database.

Methods

Data for all patients from December 2013 to November 2018 were retrieved from the NELA national database of emergency laparotomy for England and Wales. Laparoscopically attempted cases were matched 2 : 1 with open cases for propensity score derived from a logistic regression model for surgical approach; included co-variates were age, gender, predicted mortality risk, and diagnostic, procedural and surgeon variables. Groups were compared for mortality. Secondary endpoints were blood loss and duration of hospital stay.

Results

Of 116 920 patients considered, 17 040 underwent laparoscopic surgery. The most common procedures were colectomy, adhesiolysis, washout and perforated ulcer repair. Of these, 11 753 were matched exactly to 23 506 patients who had open surgery. Laparoscopically attempted surgery was associated with lower mortality (6.0 versus 9.1 per cent, P < 0.001), blood loss (less than 100 ml, 64.4 versus 52.0 per cent, P < 0.001), and duration of hospital stay (median 8 (i.q.r. 5–14) versus 10 (7–18) days, P < 0.001). Similar trends were seen when comparing only successful laparoscopic cases with open surgery, and also when comparing cases converted to open surgery with open surgery.

Conclusion

In appropriately selected patients, laparoscopy is associated with superior outcomes compared with open emergency surgery.
The preterm social brain: altered functional networks for Theory of Mind in very preterm children
Authors: Mossad Sarah I , Vandewouw Marlee M , Smith Mary Lou , Taylor Margot J
Published: January 2021

Neurodevelopmental difficulties emerge in very preterm born children (<32-week gestation) in infancy and continue to early adulthood but little is known about their social-cognitive development. This study utilized the complementary methodological advantages of both functional MRI and magnetoencephalography to examine the neural underpinnings of Theory of Mind in very preterm birth. Theory of Mind, one of the core social-cognitive skills, is the ability to attribute mental states to others, and is crucial for predicting others’ behaviours in social interactions. Eighty-three children (40 very preterm born, 24 boys, age = 8.7 ± 0.5 years, and 43 full-term born, 22 boys, age = 8.6 ± 0.5 years) completed the study. In functional MRI, both groups recruited classic Theory of Mind areas, without significant group differences. However, reduced Theory of Mind connectivity in the very preterm born group was found in magnetoencephalography in distinct theta, alpha and beta-band networks anchored in a set of brain regions that comprise the social brain. These networks included regions such as the angular gyrus, the medial pre-frontal cortex, the superior temporal gyrus and the temporal poles. Very preterm born children showed increased connectivity compared to controls in a network anchored in the occipital gyri rather than classical social-processing regions. Very preterm born children made significantly more attribution errors and mis-construed the social scenarios. Findings offer novel insight into the neural networks, supporting social cognition in very preterm born children and highlight the importance of multimodal neuroimaging to interrogate the social brain in clinical populations.
Age at First Exposure to Tackle Football is Associated with Cortical Thickness in Former Professional American Football Players
Authors: Kaufmann David , Sollmann Nico , Kaufmann Elisabeth , Veggeberg Rosanna , Tripodis Yorghos , Wrobel Pawel P , Kochsiek Janna , Martin Brett M , Lin Alexander P , Coleman Michael J , Alosco Michael L , Pasternak Ofer , Bouix Sylvain , Stern Robert A , Shenton Martha E , Koerte Inga K
Published: January 2021

Younger age at first exposure (AFE) to repetitive head impacts while playing American football increases the risk for later-life neuropsychological symptoms and brain alterations. However, it is not known whether AFE is associated with cortical thickness in American football players. Sixty-three former professional National Football League players (55.5 ± 7.7 years) with cognitive, behavioral, and mood symptoms underwent neuroimaging and neuropsychological testing. First, the association between cortical thickness and AFE was tested. Second, the relationship between clusters of decreased cortical thickness and verbal and visual memory, and composite measures of mood/behavior and attention/psychomotor speed was assessed. AFE was positively correlated with cortical thickness in the right superior frontal cortex (cluster-wise P value [CWP] = 0.0006), the left parietal cortex (CWP = 0.0003), and the occipital cortices (right: CWP = 0.0023; left: CWP = 0.0008). A positive correlation was found between cortical thickness of the right superior frontal cortex and verbal memory (R = 0.333, P = 0.019), and the right occipital cortex and visual memory (R = 0.360, P = 0.012). In conclusion, our results suggest an association between younger AFE and decreased cortical thickness, which in turn is associated with worse neuropsychological performance. Furthermore, an association between younger AFE and signs of neurodegeneration later in life in symptomatic former American football players seems likely.
Distinct and Common Large-Scale Networks of the Hippocampal Long Axis in Older Age: Links to Episodic Memory and Dopamine D2 Receptor Availability
Authors: Nordin Kristin , Nyberg Lars , Andersson Micael , Karalija Nina , Riklund Katrine , Bäckman Lars , Salami Alireza
Published: January 2021

The hippocampal longitudinal axis has been linked to dissociated functional networks relevant to episodic memory. However, the organization of axis-dependent networks and their relation to episodic memory in aging remains less explored. Moreover, age-related deterioration of the dopamine (DA) system, affecting memory and functional network properties, might constitute a source of reduced specificity of hippocampal networks in aging. Here, we characterized axis-dependent large-scale hippocampal resting-state networks, their relevance to episodic memory, and links to DA in older individuals (n = 170, 64–68 years). Partial least squares identified 2 dissociated networks differentially connected to the anterior and posterior hippocampus. These overlapped with anterior–temporal/posterior–medial networks in young adults, indicating preserved organization of axis-dependent connectivity in old age. However, axis-specific networks were overall unrelated to memory and hippocampal DA D2 receptor availability (D2DR) measured with [11C]-raclopride positron emission tomography. Further analyses identified a memory-related network modulated by hippocampal D2DR, equally connected to anterior–posterior regions. This network included medial frontal, posterior parietal, and striatal areas. The results add to the current understanding of large-scale hippocampal connectivity in aging, demonstrating axis-dependent connectivity with dissociated anterior and posterior networks, as well as a primary role in episodic memory of connectivity shared by regions along the hippocampalaxis.
The Duration of Pharyngeal Gonorrhea: A Natural History Study
Authors: Barbee Lindley A , Soge Olusegun O , Khosropour Christine M , Haglund Micaela , Yeung Winnie , Hughes James , Golden Matthew R
Published: January 2021

Background

Pharyngeal gonorrhea is relatively common. However, the duration of untreated pharyngeal gonorrhea is unknown.

Methods

From March 2016 to December 2018, we enrolled 140 men who have sex with men in a 48-week cohort study. Participants self-collected pharyngeal specimens and completed a survey weekly. Specimens were tested using a nucleic acid amplification test at the conclusion of the study. We estimated the incidence and duration of infection. We defined incident infections as 2 consecutive positive tests, and clearance as 2 consecutive negative tests; and, after visual inspection of the data, we reclassified up to 2 weeks of missing or negative tests as positive if they occurred between 2 episodes of infections. We used Kaplan-Meier estimates to define duration of infection. Finally, we report on the frequency of single-positive tests and the time between the last negative test and the positive test.

Results

Nineteen (13.6%) of 140 participants experienced 21 pharyngeal infections (incidence, 31.7/100 person-years; 95% confidence interval, 20.7–48.6/100 person-years). The estimated median duration of pharyngeal gonorrhea was 16.3 weeks (95% confidence interval, 5.1–19.7 weeks). Twenty-two men had 25 single-positive specimens, a median of 7 days (interquartile range, 7–10 days) after their last negative test.

Conclusions

The median duration of untreated pharyngeal gonorrhea is 16 weeks, more than double previous estimates. This long duration of infection likely contributes to high levels of gonorrhea transmission.
Impaired Kv7 channel activity in the central amygdala contributes to elevated sympathetic outflow in hypertension
Authors: Sheng Zhao-Fu , Zhang Hua , Zheng PeiRu , Chen Shanyan , Gu Zezong , Zhou Jing-Jing , Phaup Jeffery G , Chang Hui-Ming , Yeh Edward T H , Pan Hui-Lin , Li De-Pei
Published: January 2021

Aims

Elevated sympathetic outflow is associated with primary hypertension. However, the mechanisms involved in heightened sympathetic outflow in hypertension are unclear. The central amygdala (CeA) regulates autonomic components of emotions through projections to the brainstem. The neuronal Kv7 channel is a non-inactivating voltage-dependent K+ channel encoded by KCNQ2/3 genes involved in stabilizing the neuronal membrane potential and regulating neuronal excitability. In this study, we investigated if altered Kv7 channel activity in the CeA contributes to heightened sympathetic outflow in hypertension.

Methods and results

The mRNA and protein expression levels of Kv7.2/Kv7.3 in the CeA were significantly reduced in spontaneously hypertensive rats (SHRs) compared with Wistar–Kyoto (WKY) rats. Lowering blood pressure with coeliac ganglionectomy in SHRs did not alter Kv7.2 and Kv7.3 channel expression levels in the CeA. Fluospheres were injected into the rostral ventrolateral medulla (RVLM) to retrogradely label CeA neurons projecting to the RVLM (CeA–RVLM neurons). Kv7 channel currents recorded from CeA–RVLM neurons in brain slices were much smaller in SHRs than in WKY rats. Furthermore, the basal firing activity of CeA–RVLM neurons was significantly greater in SHRs than in WKY rats. Bath application of specific Kv7 channel blocker 10, 10-bis (4-pyridinylmethyl)-9(10H)-anthracnose (XE-991) increased the excitability of CeA–RVLM neurons in WKY rats, but not in SHRs. Microinjection of XE-991 into the CeA increased arterial blood pressure (ABP) and renal sympathetic nerve activity (RSNA), while microinjection of Kv7 channel opener QO-58 decreased ABP and RSNA, in anaesthetized WKY rats but not SHRs.

Conclusions

Our findings suggest that diminished Kv7 channel activity in the CeA contributes to elevated sympathetic outflow in primary hypertension. This novel information provides new mechanistic insight into the pathogenesis of neurogenic hypertension.
Cardiac implantable electronic devices replacements in patients followed by remote monitoring during COVID-19 lockdown
Authors: Russo Vincenzo , Rapacciuolo Antonio , Pafundi Pia Clara , de Divitiis Marcello , Volpicelli Mario , Ruocco Antonio , Rago Anna , Uran Carlo , Nappi Felice , Attena Emilio , Chianese Raffaele , Esposito Francesca , Del Giorno Giuseppe , D’Andrea Antonello , Ducceschi Valentino , Russo Giovanni , Ammendola Ernesto , Carbone Angelo , Covino Gregorio , Manzo Gianluca , Montella Gianna Maria , D’Onofrio Antonio , Nigro Gerardo
Published: January 2021

Aims 

Following coronavirus disease (COVID-19) outbreak, the Italian government adopted strict rules of lockdown and social distancing. The aim of our study was to assess the admission rate for cardiac implantable electronic devices (CIEDs) replacement procedures in Campania, the 3rd-most-populous region of Italy, during COVID-19 lockdown.

Methods and results 

Data were sourced from 16 referral hospitals in Campania from 10 March to 4 May 2020 (lockdown period) and during the same period in 2019. We retrospectively evaluated consecutive patients hospitalized for CIEDs replacement procedures during the two observational periods. The number and type of CIEDs replacement procedures among patients followed by remote monitoring (RM), the admission rate, and the type of hospital admission between the two observational periods were compared. In total, 270 consecutive patients were hospitalized for CIEDs replacement procedures over the two observation periods. Overall CIEDs replacement procedures showed a reduction rate of 41.2% during COVID-19 lockdown. Patients were equally distributed for sex (P = 0.581), and both age [median 76 years (IQR: 68–83) vs. 79 years (IQR: 68–83); P = 0.497]. Cardiac implantable electronic devices replacement procedures in patients followed by RM significantly increased (IR: +211%; P < 0.001), mainly driven by the remarkable increase rate trend of both PM (IR: +475%; P < 0.001) and implantable cardiac defibrillator replacement procedures (IR: +67%, P = 0.01), during COVID-19 lockdown compared with 2019 timeframe.

Conclusions 

We showed a significant increase trend rate of replacement procedures among CIEDs patients followed by RM, suggesting the hypothesis of its increased use to closely monitoring and to optimize the hospital admission time during COVID-19 lockdown.
Insuring crops from space: the potential of satellite-retrieved soil moisture to reduce farmers’ drought risk exposure
Authors: Vroege Willemijn , Bucheli Janic , Dalhaus Tobias , Hirschi Martin , Finger Robert
Published: January 2021

Crop producers face significant and increasing drought risks. We evaluate whether insurances based on globally and freely available satellite-retrieved soil moisture data can reduce farms’ financial drought risk exposure. We design farm individual soil moisture index insurances for wheat, maize and rapeseed production using a case study for Eastern Germany. We find that the satellite-retrieved soil moisture index insurances significantly decrease risk exposure for these crops compared to the situation where production is not insured. The satellite-retrieved index also outperforms one based on soil moisture estimates derived from meteorological measurements at ground stations. Important implications for insurers and policy makers are that they could and should develop better suited insurances. Available satellite-retrieved data can be used to increase farmers’ resilience in a changing climate.
Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei
Authors: Davey John W , Catta-Preta Carolina M C , James Sally , Forrester Sarah , Motta Maria Cristina M , Ashton Peter D , Mottram Jeremy C
Published: January 2021

Angomonas deanei is an endosymbiont-bearing trypanosomatid with several highly fragmented genome assemblies and unknown chromosome number. We present an assembly of the A. deanei nuclear genome based on Oxford Nanopore sequence that resolves into 29 complete or close-to-complete chromosomes. The assembly has several previously unknown special features; it has a supernumerary chromosome, a chromosome with a 340-kb inversion, and there is a translocation between two chromosomes. We also present an updated annotation of the chromosomal genome with 10,365 protein-coding genes, 59 transfer RNAs, 26 ribosomal RNAs, and 62 noncoding RNAs.
A high-quality de novo genome assembly of one swamp eel (Monopterus albus) strain with PacBio and Hi-C sequencing data
Authors: Tian Hai-Feng , Hu Qiao-Mu , Li Zhong
Published: January 2021

The swamp eel (Monopterus albus) is one economically important fish in China and South-Eastern Asia and a good model species to study sex inversion. There are different genetic lineages and multiple local strains of swamp eel in China, and one local strain of M. albus with deep yellow and big spots has been selected for consecutive selective breeding due to superiority in growth rate and fecundity. A high-quality reference genome of the swamp eel would be a very useful resource for future selective breeding program. In the present study, we applied PacBio single-molecule sequencing technique (SMRT) and the high-throughput chromosome conformation capture (Hi-C) technologies to assemble the M. albus genome. A 799 Mb genome was obtained with the contig N50 length of 2.4 Mb and scaffold N50 length of 67.24 Mb, indicating 110-fold and ∼31.87-fold improvement compared to the earlier released assembly (∼22.24 Kb and 2.11 Mb, respectively). Aided with Hi-C data, a total of 750 contigs were reliably assembled into 12 chromosomes. Using 22,373 protein-coding genes annotated here, the phylogenetic relationships of the swamp eel with other teleosts showed that swamp eel separated from the common ancestor of Zig-zag eel ∼49.9 million years ago, and 769 gene families were found expanded, which are mainly enriched in the immune system, sensory system, and transport and catabolism. This highly accurate, chromosome-level reference genome of M. albus obtained in this work will be used for the development of genome-scale selective breeding.
Visualizing population structure with variational autoencoders
Authors: Battey C J , Coffing Gabrielle C , Kern Andrew D
Published: January 2021

Dimensionality reduction is a common tool for visualization and inference of population structure from genotypes, but popular methods either return too many dimensions for easy plotting (PCA) or fail to preserve global geometry (t-SNE and UMAP). Here we explore the utility of variational autoencoders (VAEs)—generative machine learning models in which a pair of neural networks seek to first compress and then recreate the input data—for visualizing population genetic variation. VAEs incorporate nonlinear relationships, allow users to define the dimensionality of the latent space, and in our tests preserve global geometry better than t-SNE and UMAP. Our implementation, which we call popvae, is available as a command-line python program at github.com/kr-colab/popvae. The approach yields latent embeddings that capture subtle aspects of population structure in humans and Anopheles mosquitoes, and can generate artificial genotypes characteristic of a given sample or population.
Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes
Authors: Ouattara Mahamoudou , Tamboura Mamadou , Kambiré Dinanibè , Lê Kim Anh , Van Phan Thanh , Velusamy Srinivasan , Nguyen Hien Anh , Trang Dai Vo Thi , Lessa Fernanda C , Iijima Makiko , Nguyen Dac Trung , Schwartz Stephanie B , McGee Lesley , Traoré Rasmata Ouédraogo , Beall Bernard
Published: January 2021

The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.
Evaluation of methods for detection of β-lactamase production in MSSA
Authors: Skov Robert , Lonsway David R , Larsen Jesper , Larsen Anders Rhod , Samulioniené Jurgita , Limbago Brandi M
Published: January 2021

Objectives

Correct determination of penicillin susceptibility is pivotal for using penicillin in the treatment of Staphylococcus aureus infections. This study examines the performance of MIC determination, disc diffusion and a range of confirmatory tests for detection of penicillin susceptibility in S. aureus.

Methods

A total of 286 consecutive penicillin-susceptible S. aureus blood culture isolates as well as a challenge set of 62 MSSA isolates were investigated for the presence of the blaZ gene by PCR and subjected to penicillin-susceptibility testing using broth microdilution MIC determination, disc diffusion including reading of the zone edge, two nitrocefin tests and the cloverleaf test.

Results

Using PCR-based detection of blaZ as the gold standard, both broth microdilution MIC testing and disc diffusion testing resulted in a relatively low accuracy (82%–93%) with a sensitivity ranging from 49%–93%. Among the confirmatory tests, the cloverleaf test performed with 100% accuracy, while zone edge interpretation and nitrocefin-based tests increased the sensitivity of β-lactamase detection to 96%–98% and 82%–96% when using MIC determination or disc diffusion as primary test, respectively.

Conclusions

This investigation showed that reliable and accurate detection of β-lactamase production in S. aureus can be obtained by MIC determination or penicillin disc diffusion followed by interpretation of the zone edge as a confirmatory test for apparently penicillin-susceptible isolates. The more cumbersome cloverleaf test can also be used. Nitrocefin-based tests should not be used as the only test for confirmation of a presumptive β-lactamase-negative isolate.
Vertical restraints under Indian Competition Law: whither law and economics
Authors: Kathuria Vikas
Published: January 2021

The correct welfare assessment of vertical agreements in competition law is a difficult craft. The more mature jurisdictions such as the EU and the US have struggled to develop the optimal framework. This article scrutinizes vertical agreements cases of the Competition Commission of India (CCI) that is now 11 years old. The objective is to assist the CCI in strengthening its legal and economic framework vis-à-vis vertical agreements in its formative stage. The scrutiny of some leading cases revealed three types of infirmities in CCI’s cases. First, there are some legal ambiguities in the interpretation of the legislation. Secondly, the economic analysis is largely incoherent and truncated. Thirdly, there is a visible overreliance on the EU jurisprudence that does not conform to the legislative scheme of the Indian Competition Act, 2002. The article draws out some lessons towards the end.
Comparison of models for missing pedigree in single-step genomic prediction
Authors: Masuda Yutaka , Tsuruta Shogo , Bermann Matias , Bradford Heather L , Misztal Ignacy
Published: January 2021

Pedigree information is often missing for some animals in a breeding program. Unknown-parent groups (UPGs) are assigned to the missing parents to avoid biased genetic evaluations. Although the use of UPGs is well established for the pedigree model, it is unclear how UPGs are integrated into the inverse of the unified relationship matrix (H-inverse) required for single-step genomic best linear unbiased prediction. A generalization of the UPG model is the metafounder (MF) model. The objectives of this study were to derive 3 H-inverses and to compare genetic trends among models with UPG and MF H-inverses using a simulated purebred population. All inverses were derived using the joint density function of the random breeding values and genetic groups. The breeding values of genotyped animals (u2) were assumed to be adjusted for UPG effects (g) using matrix Q2 as u2∗=u2+Q2g before incorporating genomic information. The Quaas–Pollak-transformed (QP) H-inverse was derived using a joint density function of u2∗ and g updated with genomic information and assuming nonzero cov(u2∗,g′). The modified QP (altered) H-inverse also assumes that the genomic information updates u2∗ and g, but cov(u2∗,g′)=0. The UPG-encapsulated (EUPG) H-inverse assumed genomic information updates the distribution of u2∗. The EUPG H-inverse had the same structure as the MF H-inverse. Fifty percent of the genotyped females in the simulation had a missing dam, and missing parents were replaced with UPGs by generation. The simulation study indicated that u2∗ and g in models using the QP and altered H-inverses may be inseparable leading to potential biases in genetic trends. Models using the EUPG and MF H-inverses showed no genetic trend biases. These 2 H-inverses yielded the same genomic EBV (GEBV). The predictive ability and inflation of GEBVs from young genotyped animals were nearly identical among models using the QP, altered, EUPG, and MF H-inverses. Although the choice of H-inverse in real applications with enough data may not result in biased genetic trends, the EUPG and MF H-inverses are to be preferred because of theoretical justification and possibility to reduce biases.
Di-ANFIS: an integrated blockchain–IoT–big data-enabled framework for evaluating service supply chain performance
Authors: Bamakan Seyed Mojtaba Hosseini , Faregh Najmeh , ZareRavasan Ahad
Published: January 2021

Service supply chain management is a complex process because of its intangibility, high diversity of services, trustless settings, and uncertain conditions. However, the traditional evaluating models mostly consider the historical performance data and fail to predict and diagnose the problems’ root. This paper proposes a distributed, trustworthy, tamper-proof, and learning framework for evaluating service supply chain performance based on blockchain and adaptive network-based fuzzy inference systems (ANFIS) techniques, named Di-ANFIS. The main objectives of this research are as follows: (1) presenting hierarchical criteria of service supply chain performance to cope with the diagnosis of the problems’ root; (2) proposing a smart learning model to deal with the uncertainty conditions by a combination of neural network and fuzzy logic; and (3) introducing a distributed blockchain-based framework due to the dependence of ANFIS on big data and the lack of trust and security in the supply chain. Furthermore, the proposed six-layer conceptual framework consists of the data layer, connection layer, blockchain layer, smart layer, ANFIS layer, and application layer. This architecture creates a performance management system using the Internet of Things, smart contracts, and ANFIS based on the blockchain platform. The Di-ANFIS model provides a performance evaluation system without needing a third party and a reliable intermediary that provides an agile and diagnostic model in a smart and learning process. It also saves computing time and speeds up information flow.
Hoping for the Worst? A Paradoxical Preference for Bad News
Authors: Barasz Kate , Hagerty Serena F
Published: January 2021

Nine studies investigate when and why people may paradoxically prefer bad news—for example, hoping for an objectively worse injury or a higher-risk diagnosis over explicitly better alternatives. Using a combination of field surveys and randomized experiments, the research demonstrates that people may hope for relatively worse (vs. better) news in an effort to preemptively avoid subjectively difficult decisions (studies 1 and 2). This is because when worse news avoids a choice (study 3A)—for example, by “forcing one’s hand” or creating one dominant option that circumvents a fraught decision (study 3B)—it can relieve the decision-maker’s experience of personal responsibility (study 3C). However, because not all decisions warrant avoidance, not all decisions will elicit a preference for worse news; fewer people hope for worse news when facing subjectively easier (vs. harder) choices (studies 4A and B). Finally, this preference for worse news is not without consequence and may create perverse incentives for decision-makers, such as the tendency to forgo opportunities for improvement (studies 5A and B). The work contributes to the literature on decision avoidance and elucidates another strategy people use to circumvent difficult decisions: a propensity to hope for the worst.