bluuebunny/crossref_metadata_2025 huggingface dataset:
Accumulated Vitrified Embryos Could Be a Method for Increasing Pregnancy Rates in Patients with Poor Ovarian Response
Authors: Shin Jieun , Kwon Hwang , Choi Dong Hee , Park Chan , Kim Ji Hyang , Kim Jeehyun , Kang Youn-Jung , Koo Hwa Seon
Published: August 2022

We aimed to assess the efficacy of accumulated embryo transfer (ACC-ET) through several controlled ovarian hyperstimulation (COS) cycles to increase the rates of pregnancy in patients with poor ovarian response (POR). We retrospectively reviewed the medical records of 588 patients with POR under 43-years old who underwent embryo transfer from January 2010 to December 2015. We compared the pregnancy rate (PR), clinical pregnancy rate (CPR), and live birth rate (LBR) between ACC-ET (frozen-thawed: 47; fresh + frozen-thawed: 24) group (n = 71) and fresh ET groups (n = 517). Characteristics of ACC-ET patients were similar to those of fresh ET groups (Age: 38.1 ± 3.5 vs. 38.2 ± 3.7, p = 0.88; Anti Müllerian Hormone (AMH; ng/mL): 0.5 ± 0.4 vs. 0.6 ± 0.6, p = 0.38; follicle stimulating hormone (FSH: mIU/mL): 11.9 ± 8.0 vs. 10.8 ± 9.0, p = 0.35). The total number of transferred embryos (3.1 ± 0.9 vs. 1.5 ± 0.7, p = 0.00), PR (29.6% (21/71) vs. 18.8% (97/517), p = 0.040), and CPR (23.5% (16/68) vs. 14.0% (71/508) p = 0.047) were significantly higher in the ACC-ET group than in the fresh ET group. In addition, PR, CPR, and LBR increased with the number of ET in the fresh ET group. However, there were no significant differences observed in LBR between ACC-ET and fresh ET groups (14.9% (10/67) vs. 9.8% (50/508), p = 0.203). From our knowledge, there is no clinical evidence reported to prove that transfer of multiple embryos of adequate quality obtained through multiple cycles can compensate for the limited number of retrieved oocytes from POR patients. We concluded that ACC-ET from several COS cycles could be an alternative method to increase PR and CPR in <43-year-old patients with POR.
Transplantation of Refrozen Ovarian Cortical Strips Retrieved from a Cryopreserved Whole Ovary: Proof of Feasibility
Authors: Hossay Camille , Pirard Céline , Laurent Pascale , Kluyskens Candice , Donnez Jacques , Dolmans Marie-Madeleine
Published: August 2022

We report successful clinical outcomes after transplantation of refrozen-rethawed cortical strips from a cryopreserved whole ovary in a patient diagnosed with stage IIIb rectal adenocarcinoma. Whole ovary cryopreservation was proposed as a fertility preservation strategy in 2006 prior to radiotherapy, chemotherapy and oncological surgery. To allow for minimal residual disease screening before ovarian reimplantation, the whole ovary was thawed and dissected into cortical strips. While awaiting the results, the majority of the cortical strips were refrozen. These refrozen-rethawed cortical strips were laparoscopically grafted to 2 sites: the previously irradiated pelvic cavity and the non-irradiated extrapelvic cavity. Ovarian function resumption was assessed by recovery of menses, hormone levels, ultrasound and oocyte pick-up following controlled ovarian stimulation (COS). Restoration of ovarian function occurred 6 months after reimplantation, with recovery of menses and estradiol secretion. A total of 12 cycles were followed by the IVF department. A second reimplantation was performed 1.5 years later, since the grafts were found to have stopped functioning for >3 consecutive months. Overall, 3 fertilizable oocytes were retrieved transabdominally from the extrapelvic graft following COS, yielding 2 embryos and culminating in one fresh embryo transfer, but no pregnancy. Concerning the reimplantation site, no ovarian activity was detected in the graft placed in the previously irradiated pelvic cavity. Indeed, only fibrotic-looking tissue was observed in the pelvic site at second laparoscopy 1.5 years later, while ovarian activity was noted in the extrapelvic graft, showing a large antral follicle. All in all, transplantation of refrozen-rethawed cortical strips from a cryopreserved whole ovary can lead to ovarian function resumption and embryo development if grafted to a non-irradiated field.
Serum IL-13 Predicts Response to Golimumab in Bio-Naïve Ulcerative Colitis
Authors: Kinoshita Naohiko , Kakimoto Kazuki , Shimizu Hikaru , Nishida Koji , Numa Keijiro , Kawasaki Yuka , Tawa Hideki , Nakazawa Kei , Koshiba Ryoji , Hirata Yuki , Sakiyama Naokuni , Koubayashi Eiko , Takeuchi Toshihisa , Miyazaki Takako , Higuchi Kazuhide , Nakamura Shiro , Nishikawa Hiroki
Published: August 2022

A certain number of patients with ulcerative colitis (UC) are refractory to anti-TNF-α antibodies; biomarkers are thus needed to predict treatment efficacy. This study aimed to evaluate whether serum biomarkers that were reported to be associated with UC or anti-TNF-α antibody could predict the response to golimumab, a human anti-TNF-α monoclonal antibody, in bio-naïve patients with UC. We prospectively enrolled 23 consecutive patients with UC who were treated with golimumab. Serum samples were collected before the first golimumab dose. Eleven molecules were measured by electrochemiluminescence (ECL) or enzyme-linked immunosorbent assay (ELISA) and their association with efficacy after 10 weeks of golimumab treatment. Among the serum biomarkers, IL-13 levels were significantly higher in the non-remission group than in the remission group (p = 0.014). IL-15 levels were significantly lower in the non-response group than in the response group (p = 0.04). For clinical remission at week 10, the IL-13 0.20 concentration of pg/mL was associated with a sensitivity and specificity of 82.4% and 83.3%, respectively. Serum IL-13 may be a biomarker to predict golimumab efficacy in biologic-naïve patients with UC, and thus may help to tailor personalized treatment strategies.
Screening of Gestational Diabetes and Its Risk Factors: Pregnancy Outcome of Women with Gestational Diabetes Risk Factors According to Glycose Tolerance Test Results
Authors: Hanson Ele , Ringmets Inge , Kirss Anne , Laan Maris , Rull Kristiina
Published: August 2022

Background: Gestational diabetes mellitus (GDM) can cause maternal and neonatal health problems, and its prevalence is increasing worldwide. We assessed the screening of GDM during a 7-year period and compared the outcome of pregnancies at high risk for GDM. Methods: We analyzed non-selected pregnant women (n = 5021) receiving antenatal care in Tartu University Hospital, Estonia in 2012–2018. Pregnant women were classified based on the absence or presence of GDM risk factors as low risk (n = 2302) or high risk for GDM (n = 2719), respectively. The latter were divided into subgroups after the oral glycose tolerance test (OGTT): GDM (n = 423), normal result (n = 1357) and not tested (n = 939). Results: The proportion of women with GDM risk factors increased from 43.5% in 2012 to 57.8% in 2018, and the diagnosis of GDM more than doubled (5.2% vs. 13.7%). Pregnancies predisposed to GDM but with normal OGTT results were accompanied by an excessive gestational weight gain and increased odds to deliver a LGA baby (AOR 2.3 (CI 1.8–3.0)). Conclusions: An increasing number of pregnancies presenting GDM risk factors are diagnosed with GDM. Pregnant women with GDM risk factors are, despite normal OGTT, at risk of increased weight gain and LGA newborns.
Investigation of the Effect of Imatinib and Hydroxyurea Combination Therapy on Hematological Parameters and Gene Expression in Chronic Myeloid Leukemia (CML) Patients
Authors: Al-Amleh Esraa K. , Al-Sanabra Ola M. , Alqaisi Khalid M. , Alqaraleh Moath , Al-Nahal Jumana , Hamadneh Lama , Malki Mohammed Imad , Alhmoud Jehad F.
Published: August 2022

(1) Background: Chronic myeloid leukemia is defined as the neoplastic development of mostly myeloid cells in the bone marrow. Several treatments, including chemotherapy, radiation, hormone treatment, and immunological therapy, can be used to control this condition. The therapeutic impact on leukemic individuals varies, and the response to therapy varies between patients due to disease heterogeneity. The primary goal of this study is to compare the effects of single and Imatinib (IM) and Hydroxyurea (HU) combined treatment on hematological parameters and gene expression in CML patients. (2) Methods: This study was conducted on 51 patients, with chronic myeloid leukemia, who were admitted to Al-Basher hospital in Amman, Jordan, for follow-up. Their hematological parameters were checked and gene expression was measured for (BCL2, PP2A, CIP2A, and WT1). (3) Results: The BCL2 gene was found to be less expressed in both IM and (HU + IM) treatments as compared to the HU group alone, while PP2A gene expression was raised. Such a thing indicates that the outcome of the combined therapy method is not ideal, since PP2A activation causes CML cells to move toward the blast crisis stage. Furthermore, CIP2A gene expression revealed that IM and (HU + IM) had the same therapeutic effect and were more successful in CML patients than HU alone. With regards to the treatment effect on hematological parameters, notably in CML patients in later stages, the combination therapy (HU + IM) raised lymphocyte count, indicating a greater response to the treatment. When compared to single medicines, the combination treatment reduced the proportion of neutrophils to normal reference ranges. Platelet counts, on the other hand, dramatically decreased in both IM and (HU + IM). (4) Conclusion: Because the studied genes (BCL2, PP2A, CIP2A, and WT1) are participating in cell proliferation and death, the findings show that the examined genes are significant to understand the efficacy of various therapies. Furthermore, it was found that there was a clear effect of the clinic-based strategic treatment on hematological indicators such as WBCs, lymphocytes, neutrophils, and platelet counts.
Progress in the Pathogenesis and Treatment of Neuropsychiatric Systemic Lupus Erythematosus
Authors: Wang Minhui , Wang Ziqian , Zhang Shangzhu , Wu Yang , Zhang Li , Zhao Jiuliang , Wang Qian , Tian Xinping , Li Mengtao , Zeng Xiaofeng
Published: August 2022

Neuropsychiatric systemic lupus erythematosus (NPSLE) has a broad spectrum of subtypes with diverse severities and prognoses. Ischemic and inflammatory mechanisms, including autoantibodies and cytokine-mediated pathological processes, are key components of the pathogenesis of NPSLE. Additional brain-intrinsic elements (such as the brain barrier and resident microglia) are also important facilitators of NPSLE. An improving understanding of NPSLE may provide further options for managing this disease. The attenuation of neuropsychiatric disease in mouse models demonstrates the potential for novel targeted therapies. Conventional therapeutic algorithms include symptomatic, anti-thrombotic, and immunosuppressive agents that are only supported by observational cohort studies, therefore performing controlled clinical trials to guide further management is essential and urgent. In this review, we aimed to present the latest pathogenetic mechanisms of NPSLE and discuss the progress in its management.
Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic Study
Authors: Fernandes Ytalo , Mantovani Rafael , Reino Danilo , Novaes Arthur , Messora Michel , Gustavo Sousa Luiz , Palioto Daniela , Scombatti de Souza Sergio
Published: August 2022

The aim of this study was to evaluate the use of a new porcine bone graft in rat calvaria bone defects. Critical defects were surgically created in 24 rats that were divided into four experimental groups according to defect filling (n = 6): Control Group (CG)—blood clot; Porcine Bone Group (PG)—porcine-derived bone substitute; (BG): Bio-Oss Group (BG)–chemically and heat-treated bovine graft; Bonefill Group (BFG)—chemically treated bovine bone substitute. Euthanasia of the animals occurred 30 days after the surgery, and the area of the original surgical defect and the surrounding tissues were removed for micro-CT and histomorphometric analysis. In the micro-CT evaluation, the PG presented statistically significant differences (p < 0.05) in comparison to the CG, BG and BFG, for the parameters percentage of Bone Volume (BV/TV), Surface Bone Density (BS/TV), Number of Trabeculae (Tb.N) and Bone Connectivity (Conn), but not for Total Porosity (Po.tot) and Trabecular Thickness (Tb.Th). The histomorphometric analysis showed that the PG presented similar results to the BG regarding newly formed bone extension and to the BG and BFG regarding newly formed bone area. The porcine-derived graft presented superior microtomographic and histomorphometric results when compared to the two bovine bone substitutes.
Effects of Wind, Waves, and Currents on Icebergs and Surface Floats in the Labrador Sea: A Modeling Study
Authors: Parayil Jamseena , Demirov Entcho , Afanasyev Yakov D.
Published: August 2022

This article presents a model study of the dynamics of icebergs and surface floats in the Labrador Sea. The model was forced with data on the wind above the ocean surface, surface waves, and ocean currents. These data were obtained from the reanalysis of near-surface characteristics of the ocean and atmosphere for the year 2008. Icebergs and floats launched in an area north of the Labrador coast and to the east of Greenland generally move southeastward until they reach a boundary current “highway”. After that, they are carried by ocean currents into the central part of the subpolar North Atlantic. Simulations demonstrated that, for smaller icebergs, the primary balance is between the air and water drag, while for larger icebergs, it is between three forces: the air and water drag and the combined Coriolis and pressure forces. Floats, on the other hand, are driven mostly by the Ekman component of the surface velocity, while the geostrophic and Stokes components are less important. The significant variability in the motion of icebergs and floats is due to storms passing over the Labrador Sea, since these high-wind events introduce time-dependent dynamics.
Finetuned Super-Resolution Generative Adversarial Network (Artificial Intelligence) Model for Calcium Deblooming in Coronary Computed Tomography Angiography
Authors: Sun Zhonghua , Ng Curtise K. C.
Published: August 2022

The purpose of this study was to finetune a deep learning model, real-enhanced super-resolution generative adversarial network (Real-ESRGAN), and investigate its diagnostic value in calcified coronary plaques with the aim of suppressing blooming artifacts for the further improvement of coronary lumen assessment. We finetuned the Real-ESRGAN model and applied it to 50 patients with 184 calcified plaques detected at three main coronary arteries (left anterior descending [LAD], left circumflex [LCx] and right coronary artery [RCA]). Measurements of coronary stenosis were collected from original coronary computed tomography angiography (CCTA) and Real-ESRGAN-processed images, including Real-ESRGAN-high-resolution, Real-ESRGAN-average and Real-ESRGAN-median (Real-ESRGAN-HR, Real-ESRGAN-A and Real-ESRGAN-M) with invasive coronary angiography as the reference. Our results showed specificity and positive predictive value (PPV) of the Real-ESRGAN-processed images were improved at all of the three coronary arteries, leading to significant reduction in the false positive rates when compared to those of the original CCTA images. The specificity and PPV of the Real-ESRGAN-M images were the highest at the RCA level, with values being 80% (95% CI: 64.4%, 90.9%) and 61.9% (95% CI: 45.6%, 75.9%), although the sensitivity was reduced to 81.3% (95% CI: 54.5%, 95.9%) due to false negative results. The corresponding specificity and PPV of the Real-ESRGAN-M images were 51.9 (95% CI: 40.3%, 63.5%) and 31.5% (95% CI: 25.8%, 37.8%) at LAD, 62.5% (95% CI: 40.6%, 81.2%) and 43.8% (95% CI: 30.3%, 58.1%) at LCx, respectively. The area under the receiver operating characteristic curve was also the highest at the RCA with value of 0.76 (95% CI: 0.64, 0.89), 0.84 (95% CI: 0.73, 0.94), 0.85 (95% CI: 0.75, 0.95) and 0.73 (95% CI: 0.58, 0.89), corresponding to original CCTA, Real-ESRGAN-HR, Real-ESRGAN-A and Real-ESRGAN-M images, respectively. This study proves that the finetuned Real-ESRGAN model significantly improves the diagnostic performance of CCTA in assessing calcified plaques.
Evaluation of 3D Modeling Workflows Using Dental CBCT Data for Periodontal Regenerative Treatment
Authors: Verykokou Styliani , Ioannidis Charalabos , Angelopoulos Christos
Published: August 2022

The cone beam computed tomography (CBCT) technology is nowadays widely used in the field of dentistry and its use in the treatment of periodontal diseases has already been tackled in the international literature. At the same time, advanced segmentation methods have been introduced in state-of-the-art medical imaging software and well-established automated techniques for 3D mesh cleaning are available in 3D model editing software. However, except for the application of simple thresholding approaches for the purposes of 3D modeling of the oral cavity using CBCT data for dental applications, which does not yield accurate results, the research that has been conducted using more specialized semi-automated thresholding in dental CBCT images using existing software packages is limited. This article aims to fill the gap in the state-of-the-art research concerning the usage of CBCT data for 3D modeling of the hard tissues of the oral cavity of patients with periodontitis using existing software tools, for the needs of designing and printing 3D scaffolds for periodontal regeneration. In this context, segmentation and 3D modeling workflows using dental CBCT data that belong to a patient with periodontitis are evaluated, comparisons between the 3D models of the teeth and the alveolar bone generated through the experiments that yielded the most satisfactory results are made, and an optimal and efficient methodology for creating 3D models of teeth and alveolar bone, especially for being used as the basis for generating bioabsorbable 3D printed scaffolds of personalized treatment against periodontitis, is discussed.
Chronic Wounds and Their Therapy with Alginate-Based Dressings
Authors: Winter Raimund
Published: August 2022

This invited editorial paper is intended to provide a brief overview of the use of alginate-based wound dressings in the treatment of chronic wounds, focusing particularly on the review by Andreea Barbu et al [...]
An In-Depth Understanding of the Residential Property Value Premium of a Bikesharing Service in Portland, Oregon
Authors: Lee Sangwan
Published: August 2022

Bikesharing (BSS) is an emerging alternative mode of transportation with various benefits, such as reducing reliance on single-occupancy automobiles, boosting public health, lowering pollution levels, and enhancing accessibility. Accordingly, proximity to BSS stations can be factored into residential property values. Thus, this research attempts to explore whether (1) proximity to BSS was positively associated with residential property prices in Portland, Oregon, (2) proximity to BSS and public transportation modes showed synergistic effects on the values, and (3) the magnitude of the BSS premium intensified over time. The findings of spatial hedonic models indicate (1) a premium for proximity to BSS in both single-family and multi-family housing markets with varying magnitudes, (2) synergetic effects between BSS and public transportation modes, including light rail transit, streetcar, and bus, and (3) an unchanged premium for BSS between 2016 and 2019 with a few exceptions. The findings expand discussions around transportation-induced property value premium and offer policy implications.
Sustainable Urban Development for Heat Adaptation of Small and Medium Sized Communities
Authors: Wollschläger Niels , Zinck Felix , Schlink Uwe
Published: August 2022

Due to climate change, urban populations will be affected by worsening heat stress. The use of blue–green infrastructure can be an effective countermeasure for urban planners. In this study, the ENVI-met modelling system is used to investigate the impacts of different heat adaptation strategies, such as additional urban trees, irrigation policies, and the use of high reflective surface materials. However, under certain local conditions, these measures can have conflicting effects, e.g., trees can provide shadow but also reduce the cooling ventilation. To address such conflicts, we developed an online tool visualising urban climate simulation data and applying a new decomposition algorithm that translates the biophysical processes (i.e., radiation, ventilation, evapotranspiration, and heat storage) into surface temperature changes during heat wave events. This approach allows us to (1) identify factors responsible for heat, (2) comparatively evaluate heat mitigation of different land development scenarios, and (3) find trade-offs for conflicting adaptation measures. This online tool can support the decision-making of local stakeholders.
The Challenges of Conducting Research in Diverse Classrooms: Reflections on a Pragmatics Teaching Experiment
Authors: Ranta Leila , Zavialova Alisa
Published: August 2022

For researchers, the typical way of determining whether a pedagogical innovation works is by conducting an experiment. In migrant settings, however, experiments are more challenging to carry out due to the diversity of the learner population. Unfortunately, how to deal with these challenges is not addressed in a practical way in research methods textbooks, which typically provide a normative view of the research process. This paper aims to draw attention to the realities of classroom research carried out in the Language Instruction for Newcomers to Canada (LINC) setting. These classes consist of adult immigrants and refugees from a wide range of cultural, linguistic and educational backgrounds. We illustrate how this diversity along with other characteristics of LINC programs impact the decision-making of the researcher with respect to a pedagogical experiment focused on pragmatics. The study compared a formula-enhanced approach to teaching speech acts to the more mainstream approach aimed at raising learners’ meta-pragmatic awareness about speech act behaviour. The pre-post-delayed-post-test gains appear to favour the Formula group, but the interpretability of these results is compromised by the fact that the composition of the two classes was very different. Discussion of the limitations of this case study feeds into a broader consideration of the implications for classroom research of linguistic and cultural diversity typical of L2 educational contexts like LINC.
Genome Organization and Comparative Evolutionary Mitochondriomics of Brown Planthopper, Nilaparvata lugens Biotype 4 Using Next Generation Sequencing (NGS)
Authors: Govindharaj Guru-Pirasanna-Pandi , Babu Soumya Bharti , Choudhary Jaipal Singh , Asad Muhammad , Chidambaranathan Parameswaran , Gadratagi Basana-Gowda , Rath Prakash Chandra , Naaz Naiyar , Jaremko Mariusz , Qureshi Kamal Ahmad , Kumar Uttam
Published: August 2022

Nilaparvata lugens is the main rice pest in India. Until now, the Indian N. lugens mitochondrial genome has not been sequenced, which is a very important basis for population genetics and phylogenetic evolution studies. An attempt was made to sequence two examples of the whole mitochondrial genome of N. lugens biotype 4 from the Indian population for the first time. The mitogenomes of N. lugens are 16,072 and 16,081 bp long with 77.50% and 77.45% A + T contents, respectively, for both of the samples. The mitochondrial genome of N. lugens contains 37 genes, including 13 protein-coding genes (PCGs) (cox1-3, atp6, atp8, nad1-6, nad4l, and cob), 22 transfer RNA genes, and two ribosomal RNA (rrnS and rrnL) subunits genes, which are typical of metazoan mitogenomes. However, both samples of N. lugens mitogenome in the present study retained one extra copy of the trnC gene. Additionally, we also found 93 bp lengths for the atp8 gene in both of the samples, which were 60–70 bp less than that of the other sequenced mitogenomes of hemipteran insects. The phylogenetic analysis of the 19 delphacids mitogenome dataset yielded two identical topologies when rooted with Ugyops sp. in one clade, and the remaining species formed another clade with P. maidis and M. muiri being sisters to the remaining species. Further, the genus Nilaparvata formed a separate subclade with the other genera (Sogatella, Laodelphax, Changeondelphax, and Unkanodes) of Delphacidae. Additionally, the relationship among the biotypes of N. lugens was recovered as the present study samples (biotype-4) were separated from the three biotypes reported earlier. The present study provides the reference mitogenome for N. lugens biotype 4 that may be utilized for biotype differentiation and molecular-aspect-based future studies of N. lugens.
COVID-19 and the Pancreas: A Narrative Review
Authors: Sinagra Emanuele , Shahini Endrit , Crispino Federica , Macaione Ina , Guarnotta Valentina , Marasà Marta , Testai Sergio , Pallio Socrate , Albano Domenico , Facciorusso Antonio , Maida Marcello
Published: August 2022

The outbreak of COVID-19, initially developed in China in early December 2019, has rapidly spread to other countries and represents a public health emergency of international concern. COVID-19 has caused great concern about respiratory symptoms, but it is worth noting that it can also affect the gastrointestinal tract. However, the data on pancreatic involvement during SARS-CoV-2 infection are limited. The prevalence and severity of pancreatic damage and acute pancreatitis, as well as its pathophysiology, are still under debate. Moreover, the possible implication of pancreatic damage as an apparent adverse effect of COVID-19 therapies or vaccines are issues that need to be addressed. Finally, the COVID-19 pandemic has generated delays and organizational consequences for pancreatic surgery, an element that represent indirect damage from COVID-19. This narrative review aims to summarize and analyze all the aspects of pancreatic involvement in COVID-19 patients, trying to establish the possible underlying mechanisms and scientific evidence supporting the association between COVID-19 and pancreatic disease.
Management of Patients with Adhesive Capsulitis via Ultrasound-Guided Hydrodilatation without Concomitant Intra-Articular Lidocaine Infusion: A Single-Center Experience
Authors: Chen Yung-Chieh , Shen Shu-Huei , Chiou Hong-Jen , Wan Yung-Liang
Published: August 2022

Considering the potential chondrotoxic effects of lidocaine, this retrospective study aimed to examine whether ultrasound-guided hydrodilatation without concurrent lidocaine infusion can still provide comparable treatment benefits for patients with adhesive capsulitis (AC). Outpatient data from 104 eligible AC patients who received ultrasound-guided hydrodilatation between May 2016 and April 2021 were reviewed. A total of 59 patients received hydrodilatation with diluted corticosteroid only, while 45 patients received treatment with mixed, diluted corticosteroid and 1% lidocaine. The overall treatment outcome was documented as the percentage of clinical improvement, ranging from 0% to 100% compared to baseline, and it was ranked into poor, moderate and good treatment outcomes. The results show no significant group-wise difference in demographics, overall treatment outcome, and number of hydrodilatations, while most patients showed moderate and good treatment outcomes. Patients with lidocaine infusion did not show greater treatment benefit. Our results suggest that ultrasound-guided hydrodilatation without concurrent lidocaine infusion can still deliver good treatment benefits for AC patients, and the findings are supportive of a modified approach toward careful intra-articular local anesthetic use during management of AC in the primary care setting.
Different Involvement of ASIC and TRPA1 in Facial and Hindpaw Allodynia in Nitroglycerin-Induced Peripheral Hypersensitivities in Mice
Authors: Kim Sol-Ji , Yeo Ji-Hee , Yoon Seo-Yeon , Roh Dae-Hyun
Published: August 2022

The pathophysiological mechanism underlying migraine-associated peripheral hypersensitivity remains unclear. Acid-sensing ion channels (ASICs) and transient receptor potential ankyrin 1 (TRPA1) are known to be causative pathogenic factors of mechanical and cold allodynia, respectively. Here, we sought to investigate their involvement in cold and mechanical allodynia of the face and hindpaws, respectively, in a mouse model of repetitive nitroglycerin (NTG)-induced migraine. NTG (10 mg/kg) was administered to the mice every other day for 9 days, followed 90 min later by HC-030031 (a TRPA1 blocker) or amiloride (a non-selective ASIC blocker). Mechanical or cold sensitivity of the hindpaw and facial regions was quantified using von-Frey filaments or acetone solution, respectively. Immunohistochemistry revealed that c-Fos expression was significantly increased in the trigeminal nucleus caudalis region but not in the spinal cord. Amiloride treatment only reduced NTG-induced hindpaw mechanical allodynia, whereas HC-030031 treatment only improved facial cold allodynia. Interestingly, the number of c-Fos positive cells decreased to a similar level in each drug treatment group. These findings demonstrate that facial cold allodynia and hindpaw mechanical allodynia are differentially mediated by activation of TRPA1 and ASIC, respectively, in mice with repetitive NTG-induced hypersensitivity.
The Impact of Stroke Subtype on Recovery and Functional Outcome after Inpatient Rehabilitation: A Retrospective Analysis of Factors
Authors: Ratha Krishnan Rathi , Yeo Edgar Quan Yi , Lim Chien Joo , Chua Karen Sui Geok
Published: August 2022

The aims of this study were to compare inpatient rehabilitation outcomes between acute stroke subtypes of Cerebral Infarction (CI) and Intracerebral Hemorrhage (ICH), and to determine the predictors of discharge outcomes. A retrospective study of stroke inpatients was carried out using the discharge Functional Independence Measure (FIM) as the primary outcome measure. Relationships between stroke subtype, rehabilitation impairments, and medical complications on FIM -gain were analyzed. Altogether, 280 datasets including 211 (75.4%) CI and 69 (24.6%) ICH were analyzed. ICH patients were significantly younger than CI patients (55 years ICH vs. 64.0 years CI years, p < 0.001), had a 10-fold higher proportion needing ICU admission (ICH 82.6% vs. CI 7.6%, p < 0.001), and had significantly lower total admission FIM scores (67 points ICH vs. 74 CI points, p = 0.006), with lower motor-FIM scores in particular (38 points ICH vs. 48 points CI, p = 0.003). Significant functional improvements after inpatient rehabilitation, i.e., FIM gain, occurred regardless of stroke subtype (FIM-ICH Δ 27 vs. FIM-CI Δ 21, p = 0.05). Despite significantly worse initial stroke severity, ICH patients achieved similar functional gains, independence levels, and return-home rates compared with their CI counterparts after inpatient rehabilitation.
Gd/Mn Co-Doped CaBi4Ti4O15 Aurivillius-Phase Ceramics: Structures, Electrical Conduction and Dielectric Relaxation Behaviors
Authors: Wu Daowen , Zhou Huajiang , Li Lingfeng , Chen Yu
Published: August 2022

In this work, Gd/Mn co-doped CaBi4Ti4O15 Aurivillius-type ceramics with the formula of Ca1-xGdxBi4Ti4O15 + xGd/0.2wt%MnCO3 (abbreviated as CBT-xGd/0.2Mn) were prepared by the conventional solid-state reaction route. Firstly, the prepared ceramics were identified as the single CaBi4Ti4O15 phase with orthorhombic symmetry and the change in lattice parameters detected from the Rietveld XRD refinement demonstrated that Gd3+ was successfully substituted for Ca2+ at the A-site. SEM observations further revealed that all samples were composed of the randomly orientated plate-like grains, and the corresponding average grain size gradually decreased with Gd content (x) increasing. For all compositions studied, the frequency independence of conductivity observed above 400 °C showed a nature of ionic conduction behavior, which was predominated by the long-range migration of oxygen vacancies. Based on the correlated barrier hopping (CBH) model, the maximum barrier height WM, the dc conduction activation energy Edc, as well as the hopping conduction activation energy Ep were calculated for the CBT-xGd/0.2Mn ceramics. The composition with x = 0.06 was found to have the highest Edc value of 1.87 eV, as well as the lowest conductivity (1.8 × 10−5 S/m at 600 °C) among these compositions. The electrical modules analysis for this composition further illustrated the degree of interaction between charge carrier β increases, with an increase in temperature from 500 °C to 600 °C, and then a turn to decrease when the temperature exceeded 600 °C. The value of β reached a maximum of 0.967 at 600 °C, indicating that the dielectric relaxation behavior at this temperature was closer to the ideal Debye type.
Machine Learning Techniques for Evaluating Concrete Strength with Waste Marble Powder
Authors: Sharma Nitisha , Thakur Mohindra Singh , Sihag Parveen , Malik Mohammad Abdul , Kumar Raj , Abbas Mohamed , Saleel Chanduveetil Ahamed
Published: August 2022

The purpose of the research is to predict the compressive and flexural strengths of the concrete mix by using waste marble powder as a partial replacement of cement and sand, based on the experimental data that was acquired from the laboratory tests. In order to accomplish the goal, the models of Support vector machines, Support vector machines with bagging and Stochastic, Linear regression, and Gaussian processes were applied to the experimental data for predicting the compressive and flexural strength of concrete. The effectiveness of models was also evaluated by using statistical criteria. Therefore, it can be inferred that the gaussian process and support vector machine methods can be used to predict the respective outputs, i.e., flexural and compressive strength. The Gaussian process and Support vector machines Stochastic predicts better outcomes for flexural and compressive strength because it has a higher coefficient of correlation (0.8235 and 0.9462), lower mean absolute and root mean squared error values as (2.2808 and 1.8104) and (2.8527 and 2.3430), respectively. Results suggest that all applied techniques are reliable for predicting the compressive and flexural strength of concrete and are able to reduce the experimental work time. In comparison to input factors for this data set, the number of curing days followed by the CA, C, FA, w, and MP is essential in predicting the flexural and compressive strength of a concrete mix for this data set.
Granular Resistive Force Theory Extension for Saturated Wet Sand Ground
Authors: Ma Xinmeng , Wang Gang , Liu Kaixin , Chen Xi , Wang Jixin , Pan Biye , Wang Liquan
Published: August 2022

Amphibious environments formed from sand and water present a formidable challenge to the running motion of field robots, as the mixing of granular media (GM) and water makes the force laws of robotic legs more complicated during robot running. To this end, we extended the granular resistive force theory (RFT) to saturated wet granular media, named saturated granular RFT (SGRFT), which can be suitable for saturated wet sand submerged in water. This method can extend RFT for dry GM to saturated wet granular media (SWGM) by using the method’s velocity and depth coefficient. The force laws of the robotic legs in dry GM and SWGM were tested, compared, and analyzed. The difference in force laws between the two kinds of media, from the sensitivity to speed (10 mm/s~50 mm/s) and depth (0~60 mm), was calculated. More than 70% of the prediction results of the horizontal resistive force using SGRFT have an error of less than 6%. The effectiveness of the SGRFT in legged robots is proved by simulation and testing of three kinds of legs. The difference in force laws when running is proved by the experiments of the robot equipped with the propeller-leg in dry GM and SWGM, which is vital for amphibious robots working in shoal environments (including dry GM and SWGM ground).
Neural Fairness Blockchain Protocol Using an Elliptic Curves Lottery
Authors: Caldarola Fabio , d’Atri Gianfranco , Zanardo Enrico
Published: August 2022

To protect participants’ confidentiality, blockchains can be outfitted with anonymization methods. Observations of the underlying network traffic can identify the author of a transaction request, although these mechanisms often only consider the abstraction layer of blockchains. Previous systems either give topological confidentiality that may be compromised by an attacker in control of a large number of nodes, or provide strong cryptographic confidentiality but are so inefficient as to be practically unusable. In addition, there is no flexible mechanism to swap confidentiality for efficiency in order to accommodate practical demands. We propose a novel approach, the neural fairness protocol, which is a blockchain-based distributed ledger secured using neural networks and machine learning algorithms, enabling permissionless participation in the process of transition validation while concurrently providing strong assurance about the correct functioning of the entire network. Using cryptography and a custom implementation of elliptic curves, the protocol is designed to ensure the confidentiality of each transaction phase and peer-to-peer data exchange.
SVM and ANN Modelling Approach for the Optimization of Membrane Permeability of a Membrane Rotating Biological Contactor for Wastewater Treatment
Authors: Waqas Sharjeel , Harun Noorfidza Yub , Sambudi Nonni Soraya , Arshad Ushtar , Nordin Nik Abdul Hadi Md , Bilad Muhammad Roil , Saeed Anwar Ameen Hezam , Malik Asher Ahmed
Published: August 2022

Membrane fouling significantly hinders the widespread application of membrane technology. In the current study, a support vector machine (SVM) and artificial neural networks (ANN) modelling approach was adopted to optimize the membrane permeability in a novel membrane rotating biological contactor (MRBC). The MRBC utilizes the disk rotation mechanism to generate a shear rate at the membrane surface to scour off the foulants. The effect of operational parameters (disk rotational speed, hydraulic retention time (HRT), and sludge retention time (SRT)) was studied on the membrane permeability. ANN and SVM are machine learning algorithms that aim to predict the model based on the trained data sets. The implementation and efficacy of machine learning and statistical approaches have been demonstrated through real-time experimental results. Feed-forward ANN with the back-propagation algorithm and SVN regression models for various kernel functions were trained to augment the membrane permeability. An overall comparison of predictive models for the test data sets reveals the model’s significance. ANN modelling with 13 hidden layers gives the highest R2 value of >0.99, and the SVM model with the Bayesian optimizer approach results in R2 values higher than 0.99. The MRBC is a promising substitute for traditional suspended growth processes, which aligns with the stipulations of ecological evolution and environmentally friendly treatment.
Sr2Fe1.575Mo0.5O6-δ Promotes the Conversion of Methane to Ethylene and Ethane
Authors: Song Shiqi , Ye Lingting , Xie Kui
Published: August 2022

Oxidative coupling of methane can produce various valuable products, such as ethane and ethylene, and solid oxide electrolysis cells (SOECs) can electrolyze CH4 to produce C2H4 and C2H6. In this work, Sr2Fe1.575Mo0.5O6-δ electrode materials were prepared by impregnation and in situ precipitation, and Sr2Fe1.5Mo0.5O6-δ was taken as a reference to study the role of metal–oxide interfaces in the catalytic process. When the Fe/Sr2Fe1.575Mo0.5O6-δ interface is well constructed, the selectivity for C2 can reach 78.18% at 850 °C with a potential of 1.2 V, and the conversion rate of CH4 is 11.61%. These results further prove that a well-constructed metal–oxide interface significantly improves the catalytic activity and facilitates the reaction.
Interaction of Bortezomib with Cell Membranes Regulates Its Toxicity and Resistance to Therapy
Authors: Ramalho Maria João , Andrade Stéphanie , Loureiro Joana Angélica , Pereira Maria Carmo
Published: August 2022

Bortezomib (BTZ) is a potent proteasome inhibitor currently being used to treat multiple myeloma. However, its high toxicity and resistance to therapy severely limit the treatment outcomes. Drug–membrane interactions have a crucial role in drugs’ behavior in vivo, affecting their bioavailability and pharmacological activity. Additionally, drugs’ toxicity often occurs due to their effects on the cell membranes. Therefore, studying BTZ’s interactions with cell membranes may explain the limitations of its therapy. Due to the cell membranes’ complexity, lipid vesicles were proposed here as biomembrane models, focusing on the membrane’s main constituents. Two models with distinct composition and complexity were used, one composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and the other containing DMPC, cholesterol (Chol), and sphingomyelin (SM). BTZ’s interactions with the models were evaluated regarding the drugs’ lipophilicity, preferential location, and effects on the membrane’s physical state. The studies were conducted at different pH values (7.4 and 6.5) to mimic the normal blood circulation and the intestinal environment, respectively. BTZ revealed a high affinity for the membranes, which proved to be dependent on the drug-ionization state and the membrane complexity. Furthermore, BTZ’s interactions with the cell membranes was proven to induce changes in the membrane fluidity. This may be associated with its resistance to therapy, since the activity of efflux transmembrane proteins is dependent on the membrane’s fluidity.
Femtosecond Laser Fabrication of Microporous Membranes for Biological Applications
Authors: Volpe Annalisa , Conte Capodacqua Filippo Maria , Garzarelli Valeria , Primiceri Elisabetta , Chiriacò Maria Serena , Gaudiuso Caterina , Ferrara Francesco , Ancona Antonio
Published: August 2022

The possibility of fabricating micrometric pore size membranes is gaining great interest in many applications, from studying cell signaling, to filtration. Currently, many technologies are reported to fabricate such microsystems, the choice of which depends strictly on the substrate material and on the final application. Here, we demonstrate the capability with a single femtosecond laser source and experimental setup to fabricate micromembranes both on polymeric and multilayer metallic substrate, without the need for moulds, mask, and complex facilities. In particular, the flexibility of laser drilling was exploited to obtain microfilters with pore size of 8 and 18 µm in diameter, on metallic and polymeric substrate, respectively, and controlled distribution. For evaluating the possibility to use such laser-fabricated membranes into biological assay, their biocompatibility has been investigated. To this aim, as a proof of concept, we tested the two materials into viability tests. The culture of mammalian cells on these microfabricated membranes were studied showing their compatibility with cells.
Boron Nitride Nanoribbons Grown by Chemical Vapor Deposition for VUV Applications
Authors: Hao Jiandong , Li Ling , Gao Peng , Jiang Xiangqian , Ban Chuncheng , Shi Ningqiang
Published: August 2022

The fabrication process of vacuum ultraviolet (VUV) detectors based on traditional semiconductor materials is complex and costly. The new generation of wide-bandgap semiconductor materials greatly reduce the fabrication cost of the entire VUV detector. We use the chemical vapor deposition (CVD) method to grow boron nitride nanoribbons (BNNRs) for VUV detectors. Morphological and compositional characterization of the BNNRs was tested. VUV detector based on BNNRs exhibits strong response to VUV light with wavelengths as short as 185 nm. The photo–dark current ratio (PDCR) of this detector is 272.43, the responsivity is 0.47 nA/W, and the rise time and fall time are 0.3 s and 0.6 s. The response speed is faster than the same type of BN-based VUV detectors. This paper offers more opportunities for high-performance and low-cost VUV detectors made of wide-bandgap semiconductor materials in the future.
Study on the Micro Removal Process of Inner Surface of Cobalt Chromium Alloy Cardiovascular Stent Tubes
Authors: Song Zhuang , Zhao Yugang , Li Zhihao , Cao Chen , Liu Guangxin , Liu Qian , Zhang Xiajunyu , Dai Di , Zheng Zhilong , Zhao Chuang , Yu Hanlin
Published: August 2022

Due to the special manufacturing process of cobalt–chromium alloy cardiovascular stent tubes, there are serious surface defects in their inner walls, which affects the therapeutic effect after implantation. At the same time, the traditional processing technology cannot finish the inner wall of a cardiovascular stent tube. In light of the above problems, magnetic abrasive finishing (MAF) equipment for the inner wall of an ultra-fine and ultra-long cardiovascular stent tube is proposed, and MAF technology is used to improve the surface quality of its inner wall. High-performance spherical magnetic abrasive powders are used to finish the inner wall of a cobalt–chromium alloy cardiovascular stent tube with an inner diameter of 1.6 mm and an outer diameter of 1.8 mm. The effects of finishing time, tube rotational speed, feed speed of the magnetic pole, MAPs filling quantity, and MAP abrasive size on the surface roughness and material removal thickness of cobalt–chromium alloy cardiovascular stent tube are investigated. The results show that the surface roughness of the inner wall of the cobalt–chromium alloy cardiovascular stent decreases from 0.485 μm to 0.101 μm, and the material removal thickness of the defect layer is 4.3 μm. MAF technology is used to solve the problem of the poor surface quality of the inner walls of ultra-fine and ultra-long cobalt–chromium alloy cardiovascular stent tubes.
Potentiometric pH Sensor Based on Flexible Screen-Printable Polyaniline Composite for Textile-Based Microfluidic Applications
Authors: Laffitte Yohan , Gray Bonnie L.
Published: August 2022

Skin pH can be used for monitoring infections in a healing wound, the onset of dermatitis, and hydration in sports medicine, but many challenges exist in integrating conventional sensing materials into wearable platforms. We present the development of a flexible, textile-based, screen-printed electrode system for biosensing applications, and demonstrate flexible polyaniline (PANI) composite-based potentiometric sensors on a textile substrate for real-time pH measurement. The pH response of the optimized PANI/dodecylbenzene sulfonic acid/screen-printing ink composite is compared to electropolymerized and drop-cast PANI sensors via open circuit potential measurements. High sensitivity was observed for all sensors between pH 3–10, with a composite based on PANI emeraldine base, demonstrating sufficient response time and a linear sensitivity of −27.9 mV/pH. This exceeded prior flexible screen-printed pH sensors in which all parts of the sensor, including the pH sensing material, are screen-printed. Even better sensitivity was observed for a PANI emeraldine salt composite (−42.6 mV/pH), although the response was less linear. Furthermore, the sensor was integrated into a screen-printed microfluidic channel demonstrating sample isolation during measurement for wearable, micro cloth-based analytical devices. This is the first fully screen-printed flexible PANI composite pH sensor demonstrated on a textile substrate that can additionally be integrated with textile-based microfluidic channels.