Early progressive stages of retinopathy of prematurity: multivariate analysis of pathogenic factors
Authors:   International Scientific and Practical Center for Tissue Proliferation, Moscow, Lesovoy S.V. , Seregina E.A. ,   International Scientific and Practical Center for Tissue Proliferation, Moscow,   Dmitry Rogachev National Medical Research Center for Pediatric Hematology, Oncology and Immunology, Moscow, Vuymo T.A. ,   International Scientific and Practical Center for Tissue Proliferation, Moscow,   Center for Theoretical Problems of Physicochemical Pharmacology RAS, Moscow, Kolesnichenko I.I. ,   International Scientific and Practical Center for Tissue Proliferation, Moscow,   A.N. Frumkin Institute of Physical Chemistry and Electrochemistry RAS (IPCE), Moscow, Viner M.E. ,   International Scientific and Practical Center for Tissue Proliferation, Moscow,   Firma «Ophthalmic», Moscow,   SiriusUniversity, Sochi, Russia, Bykovskaya S.N. ,   International Scientific and Practical Center for Tissue Proliferation, Moscow,   Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, Moscow, Salmasi J.M. ,   International Scientific and Practical Center for Tissue Proliferation, Moscow,   Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, Moscow, Balashova L.M. ,   International Scientific and Practical Center for Tissue Proliferation, Moscow,   Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, Moscow
Published: June 2023

The retinopathy of prematurity (ROP) is a vasoproliferative disease causing visual impairment. Perpose. To investigate the pathogenic features of ROP. Material and methods. 1254 patients (2508 eyes), operated patients 163 (326 eyes), were included in the study; blood tests were taken from 192 patients (384 eyes). Results. Patients with lower weight, advanced stage of ROP showed greater decline in hemoglobin, lactate and thrombin time in comparison to the babies at risk; T-regulators, such as CD4+CD25highFoxp3+CD127low, correlated with gestational age and the stage of ROP; their level was increased or remained within normal range in aggressive posterior disease (AP-ROP). Serum levels of VEGFA165 and VEGFA165R1 in ROP surpassed those in babieswith congenital cataracts (p < 0.01). 19 pro-angiogenic genes (VEZF1, LTF, CSF1R, EGR1, FGF1, PTN, IFITM, HSP90AB1, HSP90B1, PRDX1, JMJD, ACTA2, XBP1, CCNG1, S100A10, IRS2, UPP1, MGP, OLFML3) and 19 antiangiogenic genes (RHBDF1, CTNNA1, F11R, PSEN2, RHBDF1, PLG, COL18A1, VHL1, EIF4A1, EIFH4, ANKRD23, MIR-16, RAB6B, TSP1, RACK1, NR2E3, TKT, VLDLR, IGFBP4) were detected. Multisensor stripping voltammetry in serum of full-term and premature babieswith ROP demonstrated significant shift of current peak height after injection of Hg (p < 0,01), Cd and Pb (p < 0,05). Conclusion. Premature patients with early stages of ROP have distinctive features of their homeostasis. Keywords: оphthalmology, CD4+CD25highFoxp3+CD127low, blood, blood serum, newborn children, cataract, retinopathy of prematurity, helth children, laboratory diagnostics, expression, antiangiogene, s factors, proangiogene, s factors, gene, multisensory inversion voltammetry, planar electrodes