bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Potential of Green Hydrogen Production and Application in the Antofagasta Region of Chile
Authors: Chavez-Angel Emigdio , Castro-Alvarez Alejandro , Sapunar Nicolas , Henríquez Francisco , Saavedra Javier , Rodríguez Sebastián , Cornejo Iván , Maxwell Lindley
Published: June 2023

Green hydrogen is gaining increasing attention as a key component of the global energy transition towards a more sustainable industry. Chile, with its vast renewable energy potential, is well positioned to become a major producer and exporter of green hydrogen. In this context, this paper explores the prospects for green hydrogen production and use in Chile. The perspectives presented in this study are primarily based on a compilation of government reports and data from the scientific literature, which primarily offer a theoretical perspective on the efficiency and cost of hydrogen production. To address the need for experimental data, an ongoing experimental project was initiated in March 2023. This project aims to assess the efficiency of hydrogen production and consumption in the Atacama Desert through the deployment of a mobile on-site laboratory for hydrogen generation. The facility is mainly composed by solar panels, electrolyzers, fuel cells, and a battery bank, and it moves through the Atacama Desert in Chile at different altitudes, from the sea level, to measure the efficiency of hydrogen generation through the energy approach. The challenges and opportunities in Chile for developing a robust green hydrogen economy are also analyzed. According to the results, Chile has remarkable renewable energy resources, particularly in solar and wind power, that could be harnessed to produce green hydrogen. Chile has also established a supportive policy framework that promotes the development of renewable energy and the adoption of green hydrogen technologies. However, there are challenges that need to be addressed, such as the high capital costs of green hydrogen production and the need for supportive infrastructure. Despite these challenges, we argue that Chile has the potential to become a leading producer and exporter of green hydrogen or derivatives such as ammonia or methanol. The country’s strategic location, political stability, and strong commitment to renewable energy provide a favorable environment for the development of a green hydrogen industry. The growing demand for clean energy and the increasing interest in decarbonization present significant opportunities for Chile to capitalize on its renewable energy resources and become a major player in the global green hydrogen market.
Selected Simulation and Experimental Studies of the Heat Transfer Process in the Railway Disc Brake in High-Speed Trains
Authors: Kukulski Jacek , Wolff Andrzej , Walczak Sławomir
Published: June 2023

The effectiveness of railway brakes strongly depends on their thermal condition. A computer simulation and experimental investigations on a full-scale dynamometric stand were chosen as an adequate analysis of the heat transfer process in brakes. The article introduces a two-dimensional, axisymmetric numerical model of the tested disc brake. Boundary conditions related to the heat generated in the friction brake and heat transferred to the environment are also presented. The transient heat transfer problem was solved using the in-house computer program of the finite element method. The article presents simulations and experimental investigations of the intensive braking of a train with an initial high speed. Temperature responses of the disc brake on the friction surface and at other selected points are shown. In addition, a thermal imaging camera was used to assess the temperature distribution on the friction surface of the disc. The results of experimental and simulation tests were preliminarily compared. Similar maximum temperature values were obtained at the end of braking with a particular discrepancy in temperature responses during the analyzed process.
Similarity Reductions, Power Series Solutions, and Conservation Laws of the Time-Fractional Mikhailov–Novikov–Wang System
Authors: Jiang Xinxin , Li Lianzhong
Published: June 2023

The current study presents a comprehensive Lie symmetry analysis for the time-fractional Mikhailov–Novikov–Wang (MNW) system with the Riemann–Liouville fractional derivative. The corresponding simplified equations with the Erdélyi–Kober fractional derivative are constructed by group invariant solutions. Furthermore, we obtain explicit solutions with the help of the power series method and show the dynamical behavior via evolutional figures. Finally, by means of Ibragimov’s new conservation theorem, the conservation laws are derived for the system.
Radionuclide Transport Simulations Supporting Proposed Borehole Waste Disposal in Israel
Authors: Swager Katherine Carol , Bourret Suzanne Michelle , Bussod Gilles Y. , Balaban Noa , Boukhalfa Hakim , Calvo Ran , Klein-BenDavid Ofra , Lucero Dolan , Reznik Itay J. , Rosenzweig Ravid , Stauffer Philip H.
Published: June 2023

A scientific collaboration between the U.S. and Israel is underway to assess the suitability of a potential site for subsurface radioactive waste disposal in the Negev Desert, Israel. The Negev Desert has several favorable attributes for geologic disposal, including an arid climate, a deep vadose zone, interlayered low-permeability lithologies, and carbonate rocks with high uranium-sorption potential. These features may provide a robust natural barrier to radionuclide migration. Geologic and laboratory characterization data from the Negev Desert are incorporated into multiphase flow and transport models, solved using PFLOTRAN, to aid in site characterization and risk analysis that will support decision-making for waste disposal in an intermediate-depth borehole design. The lithology with the greatest uranium sorption potential at the site is phosphorite. We use modeling to evaluate the ability of this layer to impact uranium transport around a proposed disposal borehole. The current objective of the simulations is focused on characterizing hypothetical leakage from waste canisters and subsequent uranium migration under three infiltration scenarios. Here, we describe a hydrogeologic model based on data from a local exploratory borehole and present results for uranium flow and transport simulations under varying infiltration scenarios. We find that under the current climate conditions, it is likely that uranium will remain in the near-field of the borehole for thousands of years. However, under a hypothesized extreme climate scenario representing an increase in infiltration by a factor of 300x above present-day values, uranium may break through the phosphorite layer and exit the base of the model domain (~200 m above the water table) within 1000 years. Simulation results have direct implications for the planning of nuclear waste disposal in the Negev Desert, and specifically in intermediate-depth boreholes.
Distribution and Morphometry of Thermocirques in the North of West Siberia, Russia
Authors: Leibman Marina , Nesterova Nina , Altukhov Maxim
Published: June 2023

The Arctic zone of West Siberia (Yamal and Gydan peninsulas) is an area with continuous permafrost and tabular ground ice close to the surface, active thermodenudation, and related landforms: retrogressive thaw slumps (RTS); in Russian referred to as thermocirques (TC). The dimensions of most TCs have not been determined so far. We use Sentinel 2 imagery to measure each TC area ranging from 0.55 to 38 ha with a median of 2.5 ha. Around 95% of TCs have an area of less than 10 ha. The largest areas are gained due to the merging of several neighboring TCs. The ArcticDEM is used to determine TC edge elevation and slope angle. In general, the Median TC of the Yamal peninsula has an area of 1.8 ha, an elevation of the edge of 17.7 m, and a slope angle of 2.5°. The Median TC of the Gydan peninsula has an area of 2.6 ha, elevation of the edge of 29.4 m, and slope angle of 3°. TCs of the Gydan peninsula occupy higher positions and slightly steeper slopes compared to TCs of the Yamal peninsula. The ranges of the median and the largest TC areas are consistent with the reported RTS dimensions in North America.
Treponema denticola Has the Potential to Cause Neurodegeneration in the Midbrain via the Periodontal Route of Infection—Narrative Review
Authors: Pisani Flavio , Pisani Valerio , Arcangeli Francesca , Harding Alice , Singhrao Simarjit
Published: June 2023

Alzheimer’s disease (AD) is a neurodegenerative disease and the most common example of dementia. The neuropathological features of AD are the abnormal deposition of extracellular amyloid-β (Aβ) and intraneuronal neurofibrillary tangles with hyperphosphorylated tau protein. It is recognized that AD starts in the frontal cerebral cortex, and then it progresses to the entorhinal cortex, the hippocampus, and the rest of the brain. However, some studies on animals suggest that AD could also progress in the reverse order starting from the midbrain and then spreading to the frontal cortex. Spirochetes are neurotrophic: From a peripheral route of infection, they can reach the brain via the midbrain. Their direct and indirect effect via the interaction of their virulence factors and the microglia potentially leads to the host peripheral nerve, the midbrain (especially the locus coeruleus), and cortical damage. On this basis, this review aims to discuss the hypothesis of the ability of Treponema denticola to damage the peripheral axons in the periodontal ligament, to evade the complemental pathway and microglial immune response, to determine the cytoskeletal impairment and therefore causing the axonal transport disruption, an altered mitochondrial migration and the consequent neuronal apoptosis. Further insights about the central neurodegeneration mechanism and Treponema denticola’s resistance to the immune response when aggregated in biofilm and its quorum sensing are suggested as a pathogenetic model for the advanced stages of AD.
SERS Signature of SARS-CoV-2 in Saliva and Nasopharyngeal Swabs: Towards Perspective COVID-19 Point-of-Care Diagnostics
Authors: Berus Sylwia M. , Nowicka Ariadna B. , Wieruszewska Julia , Niciński Krzysztof , Kowalska Aneta A. , Szymborski Tomasz R. , Dróżdż Izabela , Borowiec Maciej , Waluk Jacek , Kamińska Agnieszka
Published: June 2023

In this study, the intrinsic surface-enhanced Raman spectroscopy (SERS)-based approach coupled with chemometric analysis was adopted to establish the biochemical fingerprint of SARS-CoV-2 infected human fluids: saliva and nasopharyngeal swabs. The numerical methods, partial least squares discriminant analysis (PLS-DA) and support vector machine classification (SVMC), facilitated the spectroscopic identification of the viral-specific molecules, molecular changes, and distinct physiological signatures of pathetically altered fluids. Next, we developed the reliable classification model for fast identification and differentiation of negative CoV(−) and positive CoV(+) groups. The PLS-DA calibration model was described by a great statistical value—RMSEC and RMSECV below 0.3 and R2cal at the level of ~0.7 for both type of body fluids. The calculated diagnostic parameters for SVMC and PLS-DA at the stage of preparation of calibration model and classification of external samples simulating real diagnostic conditions evinced high accuracy, sensitivity, and specificity for saliva specimens. Here, we outlined the significant role of neopterin as the biomarker in the prediction of COVID-19 infection from nasopharyngeal swab. We also observed the increased content of nucleic acids of DNA/RNA and proteins such as ferritin as well as specific immunoglobulins. The developed SERS for SARS-CoV-2 approach allows: (i) fast, simple and non-invasive collection of analyzed specimens; (ii) fast response with the time of analysis below 15 min, and (iii) sensitive and reliable SERS-based screening of COVID-19 disease.
Synergy by Ristocetin and CXCL12 in Human Platelet Activation: Divergent Regulation by Rho/Rho-Kinase and Rac
Authors: Enomoto Yukiko , Onuma Takashi , Hori Takamitsu , Tanabe Kumiko , Ueda Kyohei , Mizutani Daisuke , Doi Tomoaki , Matsushima-Nishiwaki Rie , Ogura Shinji , Iida Hiroki , Iwama Toru , Kozawa Osamu , Tokuda Haruhiko
Published: June 2023

CXCL12, belonging to the CXC chemokine family, is a weak agonist of platelet aggregation. We previously reported that the combination of CXCL12 and collagen at low doses synergistically activates platelets via not CXCR7 but CXCR4, a specific receptor for CXCL12 on the plasma membrane. Recently, we reported that not Rho/Rho kinase, but Rac is involved in the platelet aggregation induced by this combination. Ristocetin is an activator of the von Willebrand factor that interacts with glycoprotein (GP) Ib/IX/V, which generates thromboxane A2 via phospholipase A2 activation, resulting in the release of the soluble CD40 ligand (sCD40L) from human platelets. In the present study, we investigated the effects of a combination of ristocetin and CXCL12 at low doses on human platelet activation and its underlying mechanisms. Simultaneous stimulation with ristocetin and CXCL12 at subthreshold doses synergistically induce platelet aggregation. A monoclonal antibody against not CXCR7 but CXCR4 suppressed platelet aggregation induced by the combination of ristocetin and CXCL12 at low doses. This combination induces a transient increase in the levels of both GTP-binding Rho and Rac, followed by an increase in phosphorylated cofilin. The ristocetin and CXCL12-induced platelet aggregation as well as the sCD40L release were remarkably enhanced by Y27362, an inhibitor of Rho-kinase, but reduced by NSC23766, an inhibitor of the Rac-guanine nucleotide exchange factor interaction. These results strongly suggest that the combination of ristocetin and CXCL12 at low doses synergistically induces human platelet activation via Rac and that this activation is negatively regulated by the simultaneous activation of Rho/Rho-kinase.
Fatty Acid Composition of Dry and Germinating Pollen of Gymnosperm and Angiosperm Plants
Authors: Breygina Maria , Voronkov Alexander , Ivanova Tatiana , Babushkina Ksenia
Published: June 2023

A pollen grain is a unique haploid organism characterized by a special composition and structure. The pollen of angiosperms and gymnosperms germinate in fundamentally similar ways, but the latter also have important features, including slow growth rates and lower dependence on female tissues. These features are, to some extent, due to the properties of pollen lipids, which perform a number of functions during germination. Here, we compared the absolute content and the fatty acid (FA) composition of pollen lipids of two species of flowering plants and spruce using GC-MS. The FA composition of spruce pollen differed significantly, including the predominance of saturated and monoene FAs, and a high proportion of very-long-chain FAs (VLCFAs). Significant differences between FAs from integumentary lipids (pollen coat (PC)) and lipids of gametophyte cells were found for lily and tobacco, including a very low unsaturation index of the PC. The proportion of VLCFAs in the integument was several times higher than in gametophyte cells. We found that the absolute content of lipids in lily pollen is almost three times higher than in tobacco and spruce pollen. For the first time, changes in the FA composition were analyzed during pollen germination in gymnosperms and angiosperms. The stimulating effect of H2O2 on spruce germination also led to noticeable changes in the FA content and composition of growing pollen. For tobacco in control and test samples, the FA composition was stable.
Improved Workflow for Analysis of Vascular Myocyte Time-Series and Line-Scan Ca2+ Imaging Datasets
Authors: Boskind Madison , Nelapudi Nikitha , Williamson Grace , Mendez Bobby , Juarez Rucha , Zhang Lubo , Blood Arlin B. , Wilson Christopher G. , Puglisi Jose Luis , Wilson Sean M.
Published: June 2023

Intracellular Ca2+ signals are key for the regulation of cellular processes ranging from myocyte contraction, hormonal secretion, neural transmission, cellular metabolism, transcriptional regulation, and cell proliferation. Measurement of cellular Ca2+ is routinely performed using fluorescence microscopy with biological indicators. Analysis of deterministic signals is reasonably straightforward as relevant data can be discriminated based on the timing of cellular responses. However, analysis of stochastic, slower oscillatory events, as well as rapid subcellular Ca2+ responses, takes considerable time and effort which often includes visual analysis by trained investigators, especially when studying signals arising from cells embedded in complex tissues. The purpose of the current study was to determine if full-frame time-series and line-scan image analysis workflow of Fluo-4 generated Ca2+ fluorescence data from vascular myocytes could be automated without introducing errors. This evaluation was addressed by re-analyzing a published “gold standard” full-frame time-series dataset through visual analysis of Ca2+ signals from recordings made in pulmonary arterial myocytes of en face arterial preparations. We applied a combination of data driven and statistical approaches with comparisons to our published data to assess the fidelity of the various approaches. Regions of interest with Ca2+ oscillations were detected automatically post hoc using the LCPro plug-in for ImageJ. Oscillatory signals were separated based on event durations between 4 and 40 s. These data were filtered based on cutoffs obtained from multiple methods and compared to the published manually curated “gold standard” dataset. Subcellular focal and rapid Ca2+ “spark” events from line-scan recordings were examined using SparkLab 5.8, which is a custom automated detection and analysis program. After filtering, the number of true positives, false positives, and false negatives were calculated through comparisons to visually derived “gold standard” datasets. Positive predictive value, sensitivity, and false discovery rates were calculated. There were very few significant differences between the automated and manually curated results with respect to quality of the oscillatory and Ca2+ spark events, and there were no systematic biases in the data curation or filtering techniques. The lack of statistical difference in event quality between manual data curation and statistically derived critical cutoff techniques leads us to believe that automated analysis techniques can be reliably used to analyze spatial and temporal aspects to Ca2+ imaging data, which will improve experiment workflow.
Common Genetic Variants of Response to Hepatitis B Vaccines Correlate with Risks of Chronic Infection of Hepatitis B Virus: A Community-Based Case-Control Study
Authors: Wu Tzu-Wei , Chou Chao-Liang , Chen Chuen-Fei , Wang Li-Yu
Published: June 2023

Hepatitis B (HB) vaccination effectively reduces the risks of chronic infection with the hepatitis B virus (HBV). It is unknown whether there is a common genetic determinant for response to the HB vaccine and susceptibility to chronic HBV infection. This case-control study, which included 193 chronic HBV carriers and 495 non-carriers, aimed to explore the effects of the most significant single nucleotide polymorphisms (SNPs) in response to the HB vaccine on the risks of chronic HBV infection. Out of 13 tested SNPs, the genotype distributions of four SNPs at the human leukocyte antigen (HLA) class II region, including rs34039593, rs614348, rs7770370, and rs9277535, were significantly different between HBV carriers and non-carriers. The age-sex-adjusted odds ratios (OR) of chronic HBV infection for rs34039593 TG, rs614348 TC, rs7770370 AA, and rs9277535 AA genotypes were 0.51 (95% confidence interval [CI], 0.33–0.79; p = 0.0028), 0.49 (95% CI, 0.32–0.75; p = 6.5 × 10−4), 0.33 (95% CI, 0.18–0.63; p = 7.4 × 10−4), and 0.31 (95% CI, 0.14–0.70; p = 0.0043), respectively. Multivariable analyses showed that rs614348 TC and rs7770370 AA genotypes were significantly independent protectors against chronic HBV infection. The multivariable-adjusted ORs for subjects with none, either one, or both of the protective genotypes were 1.00 (referent), 0.47 (95% CI: 0.32–0.71; p = 3.0 × 10−4), and 0.16 (95% CI: 0.05–0.54; p = 0.0032), respectively. Among eight HBeAg-positive carriers, only one of them carried a protective genotype. This study shows that response to the HB vaccine and susceptibility to chronic HBV infection share common genetic determinants and indicates that HLA class II members are the main responsible host genetic factors.
Anatomical Tissue Engineering of the Anterior Cruciate Ligament Entheses
Authors: Gögele Clemens , Hahn Judith , Schulze-Tanzil Gundula
Published: June 2023

The firm integration of anterior cruciate ligament (ACL) grafts into bones remains the most demanding challenge in ACL reconstruction, since graft loosening means graft failure. For a functional-tissue-engineered ACL substitute to be realized in future, robust bone attachment sites (entheses) have to be re-established. The latter comprise four tissue compartments (ligament, non-calcified and calcified fibrocartilage, separated by the tidemark, bone) forming a histological and biomechanical gradient at the attachment interface between the ACL and bone. The ACL enthesis is surrounded by the synovium and exposed to the intra-articular micromilieu. This review will picture and explain the peculiarities of these synovioentheseal complexes at the femoral and tibial attachment sites based on published data. Using this, emerging tissue engineering (TE) strategies addressing them will be discussed. Several material composites (e.g., polycaprolactone and silk fibroin) and manufacturing techniques (e.g., three-dimensional-/bio-printing, electrospinning, braiding and embroidering) have been applied to create zonal cell carriers (bi- or triphasic scaffolds) mimicking the ACL enthesis tissue gradients with appropriate topological parameters for zones. Functionalized or bioactive materials (e.g., collagen, tricalcium phosphate, hydroxyapatite and bioactive glass (BG)) or growth factors (e.g., bone morphogenetic proteins [BMP]-2) have been integrated to achieve the zone-dependent differentiation of precursor cells. However, the ACL entheses comprise individual (loading history) asymmetric and polar histoarchitectures. They result from the unique biomechanical microenvironment of overlapping tensile, compressive and shear forces involved in enthesis formation, maturation and maintenance. This review should provide a road map of key parameters to be considered in future in ACL interface TE approaches.
The Role of Congestion Biomarkers in Heart Failure with Reduced Ejection Fraction
Authors: Correale Michele , Fioretti Francesco , Tricarico Lucia , Croella Francesca , Brunetti Natale Daniele , Inciardi Riccardo M. , Mattioli Anna Vittoria , Nodari Savina
Published: June 2023

In heart failure with reduced ejection fraction, edema and congestion are related to reduced cardiac function. Edema and congestion are further aggravated by chronic kidney failure and pulmonary abnormalities. Furthermore, together with edema/congestion, sodium/water retention is an important sign of the progression of heart failure. Edema/congestion often anticipates clinical symptoms, such as dyspnea and hospitalization; it is associated with a reduced quality of life and a major risk of mortality. It is very important for clinicians to predict the signs of congestion with biomarkers and, mainly, to understand the pathophysiological findings that underlie edema. Not all congestions are secondary to heart failure, as in nephrotic syndrome. This review summarizes the principal evidence on the possible roles of the old and new congestion biomarkers in HFrEF patients (diagnostic, prognostic, and therapeutic roles). Furthermore, we provide a description of conditions other than congestion with increased congestion biomarkers, in order to aid in reaching a differential diagnosis. To conclude, the review focuses on how congestion biomarkers may be affected by new HF drugs (gliflozins, vericiguat, etc.) approved for HFrEF.
Association between HbA1c Levels and Fetal Macrosomia and Large for Gestational Age Babies in Women with Gestational Diabetes Mellitus: A Systematic Review and Meta-Analysis of 17,711 Women
Authors: Mou Sudipta Sarker , Gillies Clare , Hu Jiamiao , Danielli Marianna , Al Wattar Bassel Hamameeh , Khunti Kamlesh , Tan Bee Kang
Published: June 2023

Gestational diabetes mellitus (GDM) is the most common metabolic disorder in pregnancy. GDM is associated with serious maternal and fetal complications, in particular, fetal macrosomia and large for gestational age (LGA), which predisposes to a higher risk of childhood obesity and type 2 diabetes mellitus later in life. Early prediction and diagnosis of GDM leads to early interventions such as diet and lifestyle, which could mitigate the maternal and fetal complications associated with GDM. Glycated haemoglobin A1c (HbA1c) has been widely used for monitoring, screening for and diagnosing diabetes and prediabetes. Increasing evidence has also showed that HbA1c could indicate fetal glucose supply. Thus, we hypothesise that the HbA1c level at around 24 to 28 weeks may predict the development of fetal macrosomia or an LGA baby in women with GDM, which could be useful for better prevention of fetal macrosomia and LGA. We searched MEDLINE, EMBASE, Cochrane and Google Scholar databases from inception to November 2022 for relevant studies that reported at least one HbA1c level during 24–28 weeks of pregnancy and fetal macrosomia or an LGA baby. We excluded studies that were not published in the English language. No other search filters were applied during the search. Two independent reviewers selected eligible studies for meta-analysis. Two independent reviewers performed data collection and analyses. The PROSPERO registration number is CRD42018086175. A total of 23 studies were included in this systematic review. Of these, 8 papers reported data of 17,711 women with GDM that allowed for inclusion in a meta-analysis. The obtained results demonstrated the prevalence of fetal macrosomia was 7.4% and of LGA, 13.36%. Meta-analyses showed that the estimated pooled risk ratio (RR) for LGA in women with high HbA1c values compared to normal or low values was 1.70 (95% CI: 1.23–2.35), p = 0.001; and the pooled RR for fetal macrosomia was 1.45 (95% CI: 0.80 to 2.63), p = 0.215. Further research is needed to evaluate the utility of HbA1c levels in predicting the delivery of a baby with fetal macrosomia or LGA in pregnant women.
Primary Mucinous Cystadenocarcinoma of the Breast Intermixed with Pleomorphic Invasive Lobular Carcinoma: The First Report of This Rare Association
Authors: Vegni Federica , D’Alessandris Nicoletta , Santoro Angela , Angelico Giuseppe , Scaglione Giulia , Carlino Angela , Arciuolo Damiano , Valente Michele , Sfregola Stefania , Natale Maria , Sanchez Alejandro Martin , Masciullo Valeria , Zannoni Gian Franco , Mulè Antonino
Published: June 2023

Primary mucinous cystadenocarcinoma (MCA) is a rare breast carcinoma subtype showing overlapping histopathological features with mucinous cystadenocarcinoma of the ovary and pancreas. Current literature data suggest a favorable prognosis of breast MCAs despite its immunoprofile usually revealing lack of expression of estrogen receptor, progesterone receptor and HER-2 and high Ki67. As far as we know, only 36 cases have been reported in the literature to date. Its ambiguous morpho-phenotypic profile makes histological diagnosis very challenging. It must be distinguished from typical mucin-producing breast carcinomas and, above all, metastases from the same histotype in other sites (ovary, pancreas, appendix). Herein, we report the case of a primary breast MCA occurring in a 41-year-old female with peculiar histological features.
Airway Management of Orofacial Infections Originating in the Mandible
Authors: Sakkas Andreas , Weiß Christel , Zink Wolfgang , Rodriguez Camila Alejandra , Scheurer Mario , Pietzka Sebastian , Wilde Frank , Thiele Oliver Christian , Mischkowski Robert Andreas , Ebeling Marcel
Published: June 2023

The primary aim of this study was to assess the incidence of a difficult airway and emergency tracheostomy in patients with orofacial infections originating in the mandible, and a secondary aim was to determine the potential predictors of difficult intubation. This retrospective single-center study included all patients who were referred between 2015 and 2022 with an orofacial infection originating in the mandible and who were surgically drained under intubation anesthesia. The incidence of a difficult airway regarding ventilation, laryngoscopy, and intubation was analyzed descriptively. Associations between potential influencing factors and difficult intubation were examined via multivariable analysis. A total of 361 patients (mean age: 47.7 years) were included in the analysis. A difficult airway was present in 121/361 (33.5%) patients. Difficult intubation was most common in patients with infections of the massetericomandibular space (42.6%), followed by infections of the mouth floor (40%) and pterygomandibular space (23.5%). Dyspnea and stridor were not associated with the localization of infection (p = 0.6486/p = 0.4418). Multivariable analysis revealed increased age, restricted mouth opening, higher Mallampati scores, and higher Cormack–Lehane classification grades as significant predictors of difficult intubation. Higher BMI, dysphagia, dyspnea, stridor and a non-palpable mandibular rim did not influence the airway management. Patients with a difficult airway were more likely to be admitted to the ICU after surgery than patients with regular airway were (p = 0.0001). To conclude, the incidence of a difficult airway was high in patients with orofacial infections originating in the mandible. Older age, limited mouth opening, a higher Mallampati score, and a higher Cormack–Lehane grade were reliable predictors of difficult intubation.
Cognitive Impairments and Rehabilitation in Individuals with at-Risk Mental State for Psychosis
Authors: Nemoto Takahiro
Published: June 2023

In recent years, importance has been attached to early intervention in the field of healthcare, in general [...]
Transformative Justice for Elimination of Barriers to Access to Justice for Persons with Psychosocial or Intellectual Disabilities
Authors: Ruškus Jonas
Published: June 2023

By adopting the Convention on the Rights of Persons with Disabilities (the CRPD) in New York, the United Nations heralded a new epoch on how disability-related matters ought to be comprehended and addressed across the globe. The aim of this article is to argue the role and substance of the CRPD, under which each State Party has a responsibility and duty to protect, promote and implement access to justice for all persons with disabilities on equal bases with others. Systemic and structural barriers to access to justice that are faced by persons with psychosocial or intellectual disabilities are highlighted, and the determinants of them are identified including boundaries of the principle of formal equality. The human-rights-based response within the framework of obligations of the States Parties of the CRPD to ensure access to justice for persons with psychosocial or intellectual disabilities is argued, with specific consideration of the principle of transformative equality. The analysis is based on the CRPD Committee’s jurisprudence, including Concluding observations for the States Parties, General Comments, statements and guidelines.
Advanced Hybrid System for Ammonium Valorization as Liquid Fertilizer from Treated Urban Wastewaters: Validation of Natural Zeolites Pretreatment and Liquid-Liquid Membrane Contactors at Pilot Plant Scale
Authors: Mayor Álvaro , Reig Mònica , Vecino Xanel , Cortina José Luis , Valderrama César
Published: June 2023

This study evaluates a hybrid system combining zeolites as a sorption stage and a hollow fiber membrane contactor (HFMC) for ammonia (NH3) recovery from treated urban wastewater. Ion exchange with zeolites was selected as an advanced pretreatment and concentration step before the HFMC. The system was tested with wastewater treatment plant (WWTP) effluent (mainstream, 50 mg N-NH4/L) and anaerobic digestion centrates (sidestream, 600–800 mg N-NH4/L) from another WWTP. Natural zeolite, primarily clinoptilolite, demonstrated effective desorption of retained ammonium using a 2% NaOH solution in a closed-loop configuration, resulting in an ammonia-rich brine that enabled over 95% NH3 recovery using polypropylene HFMCs. A 1 m3/h demonstration plant processed both urban wastewaters, which were pretreated by ultrafiltration, removing over 90% of suspended solids and 60–65% of COD. The 2% NaOH regeneration brines (2.4–5.6 g N-NH4/L) were treated in a closed-loop HFMC pilot system, producing 10–15% N streams with potential use as liquid fertilizers. The resulting ammonium nitrate was free of heavy metals and organic micropollutants, making it suitable for use as liquid fertilizer. This comprehensive N management solution for urban wastewater applications can contribute to local economies while achieving reduced N discharge and circularity goals.
A Review of Carbapenem Resistance in Enterobacterales and Its Detection Techniques
Authors: Caliskan-Aydogan Oznur , Alocilja Evangelyn C.
Published: June 2023

Infectious disease outbreaks have caused thousands of deaths and hospitalizations, along with severe negative global economic impacts. Among these, infections caused by antimicrobial-resistant microorganisms are a major growing concern. The misuse and overuse of antimicrobials have resulted in the emergence of antimicrobial resistance (AMR) worldwide. Carbapenem-resistant Enterobacterales (CRE) are among the bacteria that need urgent attention globally. The emergence and spread of carbapenem-resistant bacteria are mainly due to the rapid dissemination of genes that encode carbapenemases through horizontal gene transfer (HGT). The rapid dissemination enables the development of host colonization and infection cases in humans who do not use the antibiotic (carbapenem) or those who are hospitalized but interacting with environments and hosts colonized with carbapenemase-producing (CP) bacteria. There are continuing efforts to characterize and differentiate carbapenem-resistant bacteria from susceptible bacteria to allow for the appropriate diagnosis, treatment, prevention, and control of infections. This review presents an overview of the factors that cause the emergence of AMR, particularly CRE, where they have been reported, and then, it outlines carbapenemases and how they are disseminated through humans, the environment, and food systems. Then, current and emerging techniques for the detection and surveillance of AMR, primarily CRE, and gaps in detection technologies are presented. This review can assist in developing prevention and control measures to minimize the spread of carbapenem resistance in the human ecosystem, including hospitals, food supply chains, and water treatment facilities. Furthermore, the development of rapid and affordable detection techniques is helpful in controlling the negative impact of infections caused by AMR/CRE. Since delays in diagnostics and appropriate antibiotic treatment for such infections lead to increased mortality rates and hospital costs, it is, therefore, imperative that rapid tests be a priority.
Laser–Metal Interaction with a Pulse Shorter than the Ion Period: Ablation Threshold, Electron Emission and Ion Explosion
Authors: Gamaly Eugene G. , Juodkazis Saulius
Published: June 2023

The laser energy per unit surface, necessary to trigger material removal, decreases with the pulse shortening, becoming pulse–time independent in the sub-picosecond range. These pulses are shorter than the electron-to-ion energy transfer time and electronic heat conduction time, minimising the energy losses. Electrons receiving an energy larger than the threshold drag the ions off the surface in the mode of electrostatic ablation. We show that a pulse shorter than the ion period (Shorter-the-Limit (StL)) ejects conduction electrons with an energy larger than the work function (from a metal), leaving the bare ions immobile in a few atomic layers. Electron emission is followed by the bare ion’s explosion, ablation, and THz radiation from the expanding plasma. We compare this phenomenon to the classic photo effect and nanocluster Coulomb explosions, and show differences and consider possibilities for detecting new modes of ablation experimentally via emitted THz radiation. We also consider the applications of high-precision nano-machining with this low intensity irradiation.
Oridonin Inhibits Mycobacterium marinum Infection-Induced Oxidative Stress In Vitro and In Vivo
Authors: Chen Guangxin , Yang Ziyue , Wen Da , Li Ping , Xiong Qiuhong , Wu Changxin
Published: June 2023

Prior to the COVID-19 pandemic, tuberculosis (TB) was the leading cause of death globally attributable to a single infectious agent, ranking higher than HIV/AIDS. Consequently, TB remains an urgent public health crisis worldwide. Oridonin (7a,20-Epoxy-1a,6b,7,14-tetrahydroxy-Kaur-16-en-15-one Isodonol, C20H28O6, Ori), derived from the Rabdosia Rrubescens plant, is a natural compound that exhibits antioxidant, anti-inflammatory, and antibacterial properties. Our objective was to investigate whether Ori’s antioxidant and antibacterial effects could be effective against the infection Mycobacterium marinum (Mm)-infected cells and zebrafish. We observed that Ori treatment significantly impeded Mm infection in lung epithelial cells, while also suppressing inflammatory response and oxidative stress in Mm-infected macrophages. Further investigation revealed that Ori supplementation inhibited the proliferation of Mm in zebrafish, as well as reducing oxidative stress levels in infected zebrafish. Additionally, Ori promoted the expression of NRF2/HO-1/NQO-1 and activated the AKT/AMPK-α1/GSK-3β signaling pathway, which are both associated with anti-inflammatory and antioxidant effects. In summary, our results demonstrate that Ori exerts inhibitory effects on Mm infection and proliferation in cells and zebrafish, respectively. Additionally, Ori regulates oxidative stress by modulating the NRF2/HO-1/NQO-1 and AKT/AMPK-α1/GSK-3β signaling pathways.
Babesia divergens Shows Equal Predilection for Human ABO Blood Types in an In Vitro Erythrocyte Preference Assay
Authors: Tijani Muyideen K. , Danielsson Lena , Storry Jill R. , Olsson Martin L. , Persson Kristina E. M.
Published: June 2023

Babesia is spread to humans via ticks or blood transfusions. Severity of Plasmodium falciparum malaria is strongly correlated to the ABO blood group of the patient. Babesia divergens is an intraerythrocytic parasite with many similarities to malaria, but the impact of ABO on the susceptibility to and progression of the infection in humans is unknown. We have now cultured B. divergens in human group A, B and O erythrocytes in vitro and measured rates of multiplication. The predilection for the different erythrocyte types was also determined using an in vitro erythrocyte preference assay when the parasites were grown in group A, B or O erythrocytes over time and then offered to invade differently stained erythrocytes of all the blood types at the same time. The results showed no difference in multiplication rates for the different blood types, and the parasite exhibited no obvious morphological differences in the different blood types. When cultured first in one blood type and then offered to grow in the others, the preference assay showed that there was no difference between the A, B or O blood groups. In conclusion, this indicates that individuals of the different ABO blood types are likely to be equally susceptible to B. divergens infections.
Elucidation of Geniposide and Crocin Accumulation and Their Biosysnthsis-Related Key Enzymes during Gardenia jasminoides Fruit Growth
Authors: Zhang Luhong , Ai Yang , Chen Yunzhu , Li Changzhu , Li Peiwang , Chen Jingzhen , Jiang Lijuan , Pan Yuhong , Sun An , Yang Yan , Liu Qiang
Published: June 2023

Gardenia jasminoides fruits are extensively grown worldwide, with a large harvest, and its major medicinal ingredients are geniposide and crocins. Research on their accumulation and biosynthsis-related enzymes is rare. In this study, the accumulation of geniposide and crocin of G. jasminoides fruits at different developmental stages were clarified by HPLC. The highest cumulative amount of geniposide was 2.035% during the unripe-fruit period, and the highest content of crocin was 1.098% during the mature-fruit period. Furthermore, transcriptome sequencing was performed. A total of 50 unigenes encoding 4 key enzymes related in geniposide biosynthsis pathways were screened, and 41 unigenes encoding 7 key enzymes in the pathways of crocin were elucidated. It was found that the expression levels of differentially expressed genes of DN67890_c0_g1_i2-encoding GGPS, which is highly related to geniposide biosynthesis, and DN81253_c0_g1_i1-encoding lcyB, DN79477_c0_g1_i2-encoding lcyE, and DN84975_c1_g7_i11-encoding CCD, which are highly related to crocin biosynthesis, were consistent with the accumulation of geniposide and crocin content, respectively. The qRT-PCR results showed that the trends of relative expression were consistent with transcribed genes. This study provides insights for understanding the geniposide and crocin accumulation and biosynthsis during fruit development in G. jasminoides.
Expression Characterization of ABCDE Class MADS-Box Genes in Brassica rapa with Different Pistil Types
Authors: Zhang Yi , Zhao Tong , Wang Yuqi , Yang Rong , Li Weiqiang , Liu Kaiwen , Sun Nairan , Hussian Iqbal , Ma Xinyan , Yu Hongrui , Zhao Kun , Chen Jisuan , Yu Xiaolin
Published: June 2023

MADS-box is a vital transcription factor family that functions in plant growth and development. Apart from APETALA2, all genes in the ABCDE model that explain the molecular mechanism of floral organ development belong to the MADS-box family. Carpel and ovule numbers in plants are essential agronomic traits that determine seed yield, and multilocular siliques have great potential for the development of high-yield varieties of Brassica. In this study, ABCDE genes in the MADS-box family from Brassica rapa were identified and characterized. Their tissue-specific expression patterns in floral organs and their differential expression in different pistil types of B. rapa were revealed by qRT-PCR. A total of 26 ABCDE genes were found to belong to the MADS-box family. Our proposed ABCDE model of B. rapa is consistent with that of Arabidopsis thaliana, indicating that ABCDE genes are functionally conserved. These results of qRT-PCR showed that the expression levels of class C and D genes were significantly different between the wild-type (wt) and tetracarpel (tetrac) mutant of B. rapa. Interestingly, the expression of the homologs of class E genes was imbalanced. Therefore, it is speculated that class C, D, and E genes are involved in developing the carpel and ovule of B. rapa. Our findings reveal the potential for the selection of candidate genes to improve yield traits in Brassica crops.
Bohemian Knotweed Reynoutria × bohemica Chrtek et Chrtková Seems Not to Rely Heavily on Allelopathy for Its Persistence in Invaded Sites in the Southwest Part of the Zagreb, Croatia
Authors: Levačić Damjana , Perković Lucia , Vuković Nina , Jelaska Sven D.
Published: June 2023

Notorious invasive Bohemian knotweed Reynoutria × bohemica Chrtek et Chrtková is a hybrid of two species, Reynoutria japonica Houtt. and Reynoutria sachalinensis (F. S. Petrop.) Nakai in T. Mori which spontaneously developed in Europe, outside the natural distribution of its parental species. Its success could potentially lie in its allelopathic activity, which was confirmed in a number of experiments conducted with the leaf and root exudates, testing their effect on the germination and growth of various test plants. Here, we tested its allelopathic potential using different concentrations of leaf exudates on two test plants, Triticum aestivum L. and Sinapis alba L., in Petri dishes and pots with soil and by growing test plants in the soil sampled in knotweed stands on the edges of stands and outside of stands. Tests in Petri dishes and pots with soil to which leaf exudates were added have shown a decrease in germination and growth in comparison to the control, hence confirming the allelopathic effect. However, this was not confirmed in a test with in situ soil samples, where no statistically significant differences were observed, neither in the growth of test plants nor in the chemical characteristics (pH, soil organic matter, humus content) of the soil. Therefore, the persistence of Bohemian knotweed at already invaded sites could be attributed to its efficient use of resources (light and nutrients) through which it outcompetes native plants.
The Diaconate of All Believers: Theology, Formation, Practice
Authors: Nessan Craig L.
Published: June 2023

This article proposes an innovative direction for Christian social practice by reclaiming and revitalizing the diaconate of all believers. While the ministry of the laity received attention in ecumenical circles in past decades, the diaconate of all believers now needs elaboration and reception. A focus on the diaconate of all believers can provide a vital theological and practical paradigm for expanding Christian social practice. In this paradigm, diaconia engages the world through the involvements of Christian people in all their roles and relationships in life, including family, work, civic engagement, and church. Christian communities need to undertake focused education and intentional formation of the diaconate of all believers through their worship and educational practices. The liturgy provides substance for this formation process. Reclaiming the diaconate of all believers as a primary expression of the church’s diaconal practice means reorienting the ministry of deacons, pastors, and bishops in relation to the universal diaconate. The recovery of the diaconate of all believers has significance for a revised ecclesiology and theology of ministry that places ministry in daily life at the forefront of Christian social practice.
Fast Semantic Segmentation of Remote Sensing Images Using a Network That Integrates Global and Local Information
Authors: Wu Boyang , Cui Jianyong , Cui Wenkai , Yuan Yirong , Ren Xiancong
Published: June 2023

Efficient processing of ultra-high-resolution images is increasingly sought after with the continuous advancement of photography and sensor technology. However, the semantic segmentation of remote sensing images lacks a satisfactory solution to optimize GPU memory utilization and the feature extraction speed. To tackle this challenge, Chen et al. introduced GLNet, a network designed to strike a better balance between GPU memory usage and segmentation accuracy when processing high-resolution images. Building upon GLNet and PFNet, our proposed method, Fast-GLNet, further enhances the feature fusion and segmentation processes. It incorporates the double feature pyramid aggregation (DFPA) module and IFS module for local and global branches, respectively, resulting in superior feature maps and optimized segmentation speed. Extensive experimentation demonstrates that Fast-GLNet achieves faster semantic segmentation while maintaining segmentation quality. Additionally, it effectively optimizes GPU memory utilization. For example, compared to GLNet, Fast-GLNet’s mIoU on the Deepglobe dataset increased from 71.6% to 72.1%, and GPU memory usage decreased from 1865 MB to 1639 MB. Notably, Fast-GLNet surpasses existing general-purpose methods, offering a superior trade-off between speed and accuracy in semantic segmentation.