bluuebunny/crossref_metadata_2025 huggingface dataset:
Exosomes and Cell Communication: From Tumour-Derived Exosomes and Their Role in Tumour Progression to the Use of Exosomal Cargo for Cancer Treatment
Authors: Nicolini Andrea , Ferrari Paola , Biava Pier Mario
Published: February 2021

Exosomes are nano-vesicle-shaped particles secreted by various cells, including cancer cells. Recently, the interest in exosomes among cancer researchers has grown enormously for their many potential roles, and many studies have focused on the bioactive molecules that they export as exosomal cargo. These molecules can function as biomarkers in diagnosis or play a relevant role in modulating the immune system and in promoting apoptosis, cancer development and progression. Others, considering exosomes potentially helpful for cancer treatment, have started to investigate them in experimental therapeutic trials. In this review, first, the biogenesis of exosomes and their main characteristics was briefly described. Then, the capability of tumour-derived exosomes and oncosomes in tumour microenvironments (TMEs) remodelling and pre-metastatic niche formation, as well as their interference with the immune system during cancer development, was examined. Finally, the potential role of exosomes for cancer therapy was discussed. Particularly, in addition, their use as carriers of natural substances and drugs with anticancer properties or carriers of boron neutron capture therapy (BNCT) and anticancer vaccines for immunotherapy, exosomes as biological reprogrammers of cancer cells have gained increased consensus. The principal aspects and the rationale of this intriguing therapeutic proposal are briefly considered.
Adaptive Therapy for Metastatic Melanoma: Predictions from Patient Calibrated Mathematical Models
Authors: Kim Eunjung , Brown Joel S. , Eroglu Zeynep , Anderson Alexander R.A.
Published: February 2021

Adaptive therapy is an evolution-based treatment approach that aims to maintain tumor volume by employing minimum effective drug doses or timed drug holidays. For successful adaptive therapy outcomes, it is critical to find the optimal timing of treatment switch points in a patient-specific manner. Here we develop a combination of mathematical models that examine interactions between drug-sensitive and resistant cells to facilitate melanoma adaptive therapy dosing and switch time points. The first model assumes genetically fixed drug-sensitive and -resistant popul tions that compete for limited resources. The second model considers phenotypic switching between drug-sensitive and -resistant cells. We calibrated each model to fit melanoma patient biomarker changes over time and predicted patient-specific adaptive therapy schedules. Overall, the models predict that adaptive therapy would have delayed time to progression by 6–25 months compared to continuous therapy with dose rates of 6–74% relative to continuous therapy. We identified predictive factors driving the clinical time gained by adaptive therapy, such as the number of initial sensitive cells, competitive effect, switching rate from resistant to sensitive cells, and sensitive cell growth rate. This study highlights that there is a range of potential patient-specific benefits of adaptive therapy and identifies parameters that modulate this benefit.
Strain Engineering for Tuning the Photocatalytic Activity of Metal-Organic Frameworks-Theoretical Study of the UiO-66 Case
Authors: Stojković Marija , Pašti Igor A.
Published: February 2021

In recent years, the class of metal-organic framework (MOF) materials emerged. These materials’ unique properties can be ascribed to their structure, containing inorganic nodes connected with organic linkers. Due to their porosity and flexibility, MOFs have become suitable for various energy-related applications, including gas storage, hydrogen production and heterogeneous catalysis, and photocatalysis. Using DFT+U calculations, we show that the substitution of metal centers in inorganic nodes and the strain engineering of UiO-66 alters the electronic and optical properties of this material. We show that applying mechanical strain on UiO-66 enables the control of absorption coefficient in the UV-Vis spectrum and the photocatalytic processes’ selectivity when reactants for several photocatalytic processes are present. The presented findings could lead to general strategies for designing novel MOFs for sustainable energy conversion applications.
Sensitivity of the Natriuretic Peptide/cGMP System to Hyperammonaemia in Rat C6 Glioma Cells and GPNT Brain Endothelial Cells
Authors: Regan Jacob T. , Mirczuk Samantha M. , Scudder Christopher J. , Stacey Emily , Khan Sabah , Worwood Michael , Powles Torinn , Dennis-Beron J. Sebastian , Ginley-Hidinger Matthew , McGonnell Imelda M. , Volk Holger A. , Strickland Rhiannon , Tivers Michael S. , Lawson Charlotte , Lipscomb Victoria J. , Fowkes Robert C.
Published: February 2021

C-type natriuretic peptide (CNP) is the major natriuretic peptide of the central nervous system and acts via its selective guanylyl cyclase-B (GC-B) receptor to regulate cGMP production in neurons, astrocytes and endothelial cells. CNP is implicated in the regulation of neurogenesis, axonal bifurcation, as well as learning and memory. Several neurological disorders result in toxic concentrations of ammonia (hyperammonaemia), which can adversely affect astrocyte function. However, the relationship between CNP and hyperammonaemia is poorly understood. Here, we examine the molecular and pharmacological control of CNP in rat C6 glioma cells and rat GPNT brain endothelial cells, under conditions of hyperammonaemia. Concentration-dependent inhibition of C6 glioma cell proliferation by hyperammonaemia was unaffected by CNP co-treatment. Furthermore, hyperammonaemia pre-treatment (for 1 h and 24 h) caused a significant inhibition in subsequent CNP-stimulated cGMP accumulation in both C6 and GPNT cells, whereas nitric-oxide-dependent cGMP accumulation was not affected. CNP-stimulated cGMP efflux from C6 glioma cells was significantly reduced under conditions of hyperammonaemia, potentially via a mechanism involving changed in phosphodiesterase expression. Hyperammonaemia-stimulated ROS production was unaffected by CNP but enhanced by a nitric oxide donor in C6 cells. Extracellular vesicle production from C6 cells was enhanced by hyperammonaemia, and these vesicles caused impaired CNP-stimulated cGMP signalling in GPNT cells. Collectively, these data demonstrate functional interaction between CNP signalling and hyperammonaemia in C6 glioma and GPNT cells, but the exact mechanisms remain to be established.
Aldehyde Dehydrogenase, a Therapeutic Target in Chordoma: Analysis in 3D Cellular Models
Authors: Locquet Marie-Anaïs , Dechaume Anne-Lise , Berchard Paul , Abbes Lhorra , Pissaloux Daniel , Tirode Franck , Ramos Inès , Bedoucha Julie , Valantin Julie , Karanian Marie , Perret Raul , Gille Olivier , Blay Jean-Yves , Dutour Aurélie
Published: February 2021

Chordomas are rare, slow-growing tumors of the axial skeleton. These tumors are locally aggressive and refractory to conventional therapies. Radical surgery and radiation remain the first-line treatments. Despite these aggressive treatments, chordomas often recur and second-line treatment options are limited. The mechanisms underlying chordoma radioresistance remain unknown, although several radioresistant cancer cells have been shown to respond favorably to aldehyde dehydrogenase (ALDH) inhibition. The study of chordoma has been delayed by small patient cohorts and few available models due to the scarcity of these tumors. We thus created cellular 3D models of chordoma by using low-adherence culture systems. Then, we evaluated their radiosensitivity using colony-forming and spheroid size assays. Finally, we determined whether pharmacologically inhibiting ALDH increased their radiosensitivity. We found that 3D cellular models of chordoma (derived from primary, relapse, and metastatic tumors) reproduce the histological and gene expression features of the disease. The metastatic, relapse, and primary spheroids displayed high, medium, and low radioresistance, respectively. Moreover, inhibiting ALDH decreased the radioresistance in all three models.
The Liver under the Spotlight: Bile Acids and Oxysterols as Pivotal Actors Controlling Metabolism
Authors: Lefort Charlotte , Cani Patrice D.
Published: February 2021

Among the myriad of molecules produced by the liver, both bile acids and their precursors, the oxysterols are becoming pivotal bioactive lipids which have been underestimated for a long time. Their actions are ranging from regulation of energy homeostasis (i.e., glucose and lipid metabolism) to inflammation and immunity, thereby opening the avenue to new treatments to tackle metabolic disorders associated with obesity (e.g., type 2 diabetes and hepatic steatosis) and inflammatory diseases. Here, we review the biosynthesis of these endocrine factors including their interconnection with the gut microbiota and their impact on host homeostasis as well as their attractive potential for the development of therapeutic strategies for metabolic disorders.
Evaluation of Browning Agents on the White Adipogenesis of Bone Marrow Mesenchymal Stromal Cells: A Contribution to Fighting Obesity
Authors: Di Maio Girolamo , Alessio Nicola , Demirsoy Ibrahim Halil , Peluso Gianfranco , Perrotta Silverio , Monda Marcellino , Di Bernardo Giovanni
Published: February 2021

Brown-like adipocytes can be induced in white fat depots by a different environmental or drug stimuli, known as “browning” or “beiging”. These brite adipocytes express thermogenin UCP1 protein and show different metabolic advantages, such as the ability to acquire a thermogenic phenotype corresponding to standard brown adipocytes that counteracts obesity. In this research, we evaluated the effects of several browning agents during white adipocyte differentiation of bone marrow-derived mesenchymal stromal cells (MSCs). Our in vitro findings identified two compounds that may warrant further in vivo investigation as possible anti-obesity drugs. We found that rosiglitazone and sildenafil are the most promising drug candidates for a browning treatment of obesity. These drugs are already available on the market for treating diabetes and erectile dysfunction, respectively. Thus, their off-label use may be contemplated, but it must be emphasized that some severe side effects are associated with use of these drugs.
Examining the Relationship between Circulating CD4− CD8− Double-Negative T Cells and Outcomes of Immuno-Checkpoint Inhibitor Therapy—Looking for Biomarkers and Therapeutic Targets in Metastatic Melanoma
Authors: Strippoli Sabino , Fanizzi Annarita , Negri Antonio , Quaresmini Davide , Nardone Annalisa , Armenio Andrea , Sciacovelli Angela Monica , Massafra Raffaella , De Risi Ivana , De Tullio Giacoma , Albano Anna , Guida Michele
Published: February 2021

Background: The role of circulating CD4−/CD8− double-negative T cells (DNTs) in the immune response to melanoma is poorly understood, as are the effects of checkpoint inhibitors on T cell subpopulations. Methods: We performed a basal and longitudinal assessment of circulating immune cells, including DNTs, in metastatic melanoma patients treated with checkpoint blockade in a single-center cohort, and examined the correlations levels of immune cells with clinical features and therapy outcomes. Results: Sixty-eight patients (48 ipilimumab, 20 PD1 inhibitors) were enrolled in the study. Our analysis indicated that better outcomes were associated with normal LDH, fewer than three metastatic sites, an ECOG performance status of 0, M1a stage, lower WBC and a higher lymphocyte count. The increase in lymphocyte count and decrease of DNTs were significantly associated with the achievement of an overall response. The median value of DNT decreased while the CD4+ and NK cells increased in patients that responded to treatment compare to those who did not respond to treatment. Conclusions: DNT cells change during treatment with checkpoint inhibitors and may be adept at sensing the immune response to melanoma. The complementary variation of DNT cells with respect to CD4+ and other immune actors may improve the reliability of lymphocyte assessment. Further investigation of DNT as a potential target in checkpoint inhibitor resistant melanoma is warranted.
Invertebrate Models Untangle the Mechanism of Neurodegeneration in Parkinson’s Disease
Authors: Surguchov Andrei
Published: February 2021

Parkinson’s disease (PD) is the second most common neurodegenerative disease, afflicting ~10 million people worldwide. Although several genes linked to PD are currently identified, PD remains primarily an idiopathic disorder. Neuronal protein α-synuclein is a major player in disease progression of both genetic and idiopathic forms of PD. However, it cannot alone explain underlying pathological processes. Recent studies demonstrate that many other risk factors can accelerate or further worsen brain dysfunction in PD patients. Several PD models, including non-mammalian eukaryotic organisms, have been developed to identify and characterize these factors. This review discusses recent findings in three PD model organisms, i.e., yeast, Drosophila, and Caenorhabditis elegans, that opened new mechanisms and identified novel contributors to this disorder. These non-mammalian models share many conserved molecular pathways and cellular processes with humans. New players affecting PD pathogenesis include previously unknown genes/proteins, novel signaling pathways, and low molecular weight substances. These findings might respond to the urgent need to discover novel drug targets for PD treatment and new biomarkers for early diagnostics of this disease. Since the study of neurodegeneration using simple eukaryotic organisms brought a huge amount of information, we include only the most recent or the most important relevant data.
Tissue–Resident Memory T Cells in Chronic Inflammation—Local Cells with Systemic Effects?
Authors: Samat Anoushka Ashok Kumar , van der Geest Jolijn , Vastert Sebastiaan J. , van Loosdregt Jorg , van Wijk Femke
Published: February 2021

Chronic inflammatory diseases such as rheumatoid arthritis (RA), Juvenile Idiopathic Arthritis (JIA), psoriasis, and inflammatory bowel disease (IBD) are characterized by systemic as well as local tissue inflammation, often with a relapsing-remitting course. Tissue–resident memory T cells (TRM) enter non-lymphoid tissue (NLT) as part of the anamnestic immune response, especially in barrier tissues, and have been proposed to fuel chronic inflammation. TRM display a distinct gene expression profile, including upregulation of CD69 and downregulation of CD62L, CCR7, and S1PR1. However, not all TRM are consistent with this profile, and it is now more evident that the TRM compartment comprises a heterogeneous population, with differences in their function and activation state. Interestingly, the paradigm of TRM remaining resident in NLT has also been challenged. T cells with TRM characteristics were identified in both lymph and circulation in murine and human studies, displaying similarities with circulating memory T cells. This suggests that re-activated TRM are capable of retrograde migration from NLT via differential gene expression, mediating tissue egress and circulation. Circulating ‘ex-TRM’ retain a propensity for return to NLT, especially to their tissue of origin. Additionally, memory T cells with TRM characteristics have been identified in blood from patients with chronic inflammatory disease, leading to the hypothesis that TRM egress from inflamed tissue as well. The presence of TRM in both tissue and circulation has important implications for the development of novel therapies targeting chronic inflammation, and circulating ‘ex-TRM’ may provide a vital diagnostic tool in the form of biomarkers. This review elaborates on the recent developments in the field of TRM in the context of chronic inflammatory diseases.
Apoptosis of Hepatocytes: Relevance for HIV-Infected Patients under Treatment
Authors: Gruevska Aleksandra , Moragrega Ángela B. , Cossarizza Andrea , Esplugues Juan V. , Blas-García Ana , Apostolova Nadezda
Published: February 2021

Due to medical advances over the past few decades, human immunodeficiency virus (HIV) infection, once a devastatingly mortal pandemic, has become a manageable chronic condition. However, available antiretroviral treatments (cART) cannot fully restore immune health and, consequently, a number of inflammation-associated and/or immunodeficiency complications have manifested themselves in treated HIV-infected patients. Among these chronic, non-AIDS (acquired immune deficiency syndrome)-related conditions, liver disease is one of the deadliest, proving to be fatal for 15–17% of these individuals. Aside from the presence of liver-related comorbidities, including metabolic disturbances and co-infections, HIV itself and the adverse effects of cART are the main factors that contribute to hepatic cell injury, inflammation, and fibrosis. Among the molecular mechanisms that are activated in the liver during HIV infection, apoptotic cell death of hepatocytes stands out as a key pathogenic player. In this review, we will discuss the evidence and potential mechanisms involved in the apoptosis of hepatocytes induced by HIV, HIV-encoded proteins, or cART. Some antiretroviral drugs, especially the older generation, can induce apoptosis of hepatic cells, which occurs through a variety of mechanisms, such as mitochondrial dysfunction, increased production of reactive oxygen species (ROS), and induction of endoplasmic reticulum (ER) stress and unfolded protein response (UPR), all of which ultimately lead to caspase activation and cell death.
Differential Function of a Novel Population of the CD19+CD24hiCD38hi Bregs in Psoriasis and Multiple Myeloma
Authors: Bartosińska Joanna , Purkot Joanna , Karczmarczyk Agnieszka , Chojnacki Michał , Zaleska Joanna , Własiuk Paulina , Grząśko Norbert , Morawska Marta , Walter-Croneck Adam , Usnarska-Zubkiewicz Lidia , Zielińska Patrycja , Jamroziak Krzysztof , Kowal Małgorzata , Krasowska Dorota , Chodorowska Grażyna , Giannopoulos Krzysztof
Published: February 2021

Psoriasis (Ps), an autoimmune disease, and multiple myeloma (MM), a blood neoplasm, are characterized by immune dysregulation resulting from the imbalance between the effector and regulatory cells, including B regulatory (Breg) lymphocytes. Peripheral blood samples from 80 Ps patients, 17 relapsed/refractory MM patients before and after daratumumab (anti-CD38 monoclonal antibody) treatment, 23 healthy volunteers (HVs), and bone marrow samples from 59 MM patients were used in the study. Bregs were determined by flow cytometry using CD19, CD24, and CD38. Intracellular production of interleukin-10 (IL-10) was assessed by flow cytometry after CD40L, LPS, and CpG stimulation. IL-10 serum or plasma concentrations were tested using ELISA method. The percentage of CD19+CD24hiCD38hi Bregs was not different whereas the production of IL-10 in Bregs was significantly higher in Ps patients in comparison with HVs. The percentage of CD19+CD24hiCD38hi Bregs in MM patients was significantly higher than in HVs (p < 0.0001). The percentage of CD19+CD24hiCD38hi Bregs was significantly higher in MM patients with the ISS stage I (p = 0.0233) while IL-10 production in Bregs was significantly higher in ISS stage III (p = 0.0165). IL-10 serum or plasma concentration was significantly higher in Ps and MM patients when compared to HVs (p < 0.0001). Following the treatment with daratumumab the percentages of CD19+CD24hiCD38hi Bregs significantly decreased (p < 0.0003). Here, in the two opposite immune conditions, despite the differences in percentages of Bregs in Ps and MM we have identified some similarities in the IL-10 producing Bregs. Effective treatment of daratumumab besides the anti-myeloma effect was accompanied by the eradication of Bregs.
Using Mediation Analysis to Understand How Treatments for Paediatric Pain Work: A Systematic Review and Recommendations for Future Research
Authors: Leake Hayley B. , Moseley G. Lorimer , Stanton Tasha R. , Heathcote Lauren C. , Pate Joshua W. , Wewege Michael A. , Lee Hopin
Published: February 2021

Clinicians have an increasing number of evidence-based interventions to treat pain in youth. Mediation analysis offers a way of investigating how interventions work, by examining the extent to which an intermediate variable, or mediator, explains the effect of an intervention. This systematic review examined studies that used mediation analysis to investigate mechanisms of interventions on pain-relevant outcomes for youth (3–18 years) with acute or chronic pain, and provides recommendations for future mediation research in this field. We searched five electronic databases for clinical trials or observational longitudinal studies that included a comparison group and conducted mediation analyses of interventions on youth and assessed pain outcomes. We found six studies (N = 635), which included a total of 53 mediation models examining how interventions affect pain-relevant outcomes for youth. Five studies were secondary analyses of randomized controlled trials of psychological interventions for chronic pain; one was a longitudinal observational study of morphine for acute pain. The pain conditions studied were irritable bowel syndrome, functional abdominal pain, juvenile fibromyalgia, mixed chronic pain, and post-operative pain. Fourteen putative mediators were tested, of which three partially mediated treatment effect; seven did not significantly mediate treatment effect and four had mixed results. Methodological and reporting limitations were common. There are substantial gaps in the field with respect to investigating, and therefore understanding, how paediatric interventions work.
Tailoring Crystalline Structure of Titanium Oxide Films for Optical Applications Using Non-Biased Filtered Cathodic Vacuum Arc Deposition at Room Temperature
Authors: Guillén Elena , Krause Matthias , Heras Irene , Rincón-Llorente Gonzalo , Escobar-Galindo Ramón
Published: February 2021

Titanium oxide films were deposited at room temperature and with no applied bias using a filtered cathodic vacuum arc (FCVA) system in a reactive oxygen environment. The dependence of film growth on two process parameters, the working pressure (Pw) and the O2 partial pressure (pO2), is described in detail. The composition, morphological features, crystalline structure, and optical properties of the deposited films were systematically studied by Rutherford Back Scattering (RBS), Scanning Electron Microscopy (SEM), X-Ray diffraction (XRD), Raman Spectroscopy, UV-vis spectroscopy, and spectroscopic ellipsometry. This systematic investigation allowed the identification of three different groups or growth regimes according to the stoichiometry and the phase structure of the titanium oxide films. RBS analysis revealed that a wide range of TiOx stoichiometries (0.6 < × < 2.2) were obtained, including oxygen-deficient, stoichiometric TiO2 and oxygen-rich films. TiO, Ti2O3, rutile-type TiO2, and amorphous TiO2 phase structures could be achieved, as confirmed both by Raman and XRD. Therefore, the results showed a highly versatile approach, in which different titanium oxide stoichiometries and crystalline phases especially suited for diverse optical applications can be obtained by changing only two process parameters, in a process at room temperature and without applied bias. Of particular interest are crystalline rutile films with high density to be used in ultra-high reflectance metal-dielectric multilayered mirrors, and reduced-TiO2 rutile samples with absorption in the visible range as a very promising photocatalyst material.
Effects of Cr2O3 Content on Microstructure and Mechanical Properties of Al2O3 Matrix Composites
Authors: Cui Kunkun , Zhang Yingyi , Fu Tao , Hussain Shahid , Saad Algarni Tahani , Wang Jie , Zhang Xu , Ali Shafaqat
Published: February 2021

Al2O3-Cr2O3 refractories are completely substitution solid solutions and can effectively resist slag erosion when used as an industrial furnace lining. In order to provide suitable chromium corundum refractory with excellent slag resistance and mechanical properties for smelting reduction ironmaking, Al2O3-Cr2O3 samples with different mass percentages (0, 10, 20, 30, 40 wt.%) of Cr2O3 were prepared by a normal pressure sintering process to study its sintering properties, mechanical properties, thermal shock resistance, and microstructure. The results of densification behavior showed that the introduction of Cr2O3 deteriorates the compactness, the relative density and volume shrinkage rate of the composite material decrease with the increase of the Cr2O3 content, and the apparent porosity increases accordingly. In terms of mechanical properties, the hardness, compressive strength, and flexural strength of Al2O3-Cr2O3 material decrease gradually with the increase of Cr2O3. After 10 and 20 thermal shock cycles, the flexural strengths of the samples all decreased. With the increase of Cr2O3 in these samples, the loss rate of flexural strength gradually increased. Considering the slag resistance and mechanical properties of the composite material, the Al2O3-Cr2O3 composite refractory with Cr2O3 content of 20–30% can meet the requirements of smelting reduction iron making kiln lining.
Bacterial Adhesion on Glass–Ionomer Cements and Micro/Nano Hybrid Composite Dental Surfaces
Authors: Bohinc Klemen , Tintor Erna , Kovačević Davor , Vidrih Rajko , Zore Anamarija , Abram Anže , Kojić Željka , Obradović Marija , Veselinović Valentina , Dolić Olivera
Published: February 2021

Dental restorations need to reproduce the aspect of the natural teeth of the patient, and must be non-toxic, biocompatible, and have good mechanical properties so that they can last for longer. The aim of this study was to determine the extent of bacterial adhesion of Streptococcus mutans on four different dental material surfaces, i.e., two glass–ionomer cements (Fuji conventional and Fuji hybrid) and two ceramic composites (Micro hybrid composite and Nano hybrid composite). To understand the bacterial adhesion on these four different dental materials, various surface properties were measured: roughness, contact angle, CIE color parameters and zeta potential. We found that the greatest adhesion extent was obtained for the Nano hybrid composite surface. The pronounced adhesion is the interplay between the relatively high roughness and hydrophilicity of the Nano hybrid composite surface. Color changes upon immersing ceramic composites in red wine and black tea proved that both beverages adhered to them. Black tea adhered more intensively than wine, and showed a higher inhibitory effect on the growth of Streptococcus mutans and Staphylococcus aureus.
Conservation: A New Open Access Journal for Rapid Dissemination of the Transdisciplinary Dimensions of Biodiversity Conservation
Authors: Margalida Antoni , Luiselli Luca , Tella José L. , Zhao Shuqing
Published: February 2021

We are pleased to launch the new peer-reviewed open access journal, Conservation, published by MDPI (Multidisciplinary Digital Publishing Institute), which offers an exciting new opportunity to publish comprehensive reviews, original research articles, communications, case reports, letters, commentaries, and other perspectives related to the biological, sociological, ethical, economic, methodological, and other transdisciplinary dimensions of conservation [...]
2.0 μm Ultra Broadband Emission from Tm3+/Ho3+ Co-Doped Gallium Tellurite Glasses for Broadband Light Sources and Tunable Fiber Lasers
Authors: Yuan Jian , Wang Weichao , Ye Yichen , Deng Tingting , Huang Yizhao , Gu Shitao , Chen Yuanbin , Xiao Peng
Published: February 2021

A flat 2.0 μm ultra broadband emission with a full width at half maximum (FWHM) of 329 nm is achieved in 1 mol.% Tm2O3 and 0.05 mol.% Ho2O3 co-doped gallium tellurite glasses upon the excitation of an 808 nm laser diode. The influence of Tm3+ and Ho3+ contents on 2.0 μm spectroscopic properties of gallium tellurite glasses is minutely investigated by absorption spectra, emission spectra, and lifetime measurement. In addition, emission cross section and gain coefficient of Ho3+ ions at 2.0 μm are calculated, and the maximum values reach 8.2 × 10−21 cm2 and 1.54 cm−1, respectively. Moreover, forward and backward energy transfer probability between Tm3+ and Ho3+ ions are qualitatively evaluated by the extended spectral overlap method. Large ratio of the forward energy transfer from Tm3+ to Ho3+ to the backward one (19.7) and high forward energy transfer coefficient (6.22 × 1039 cm6/s) are responsible for effective 2.0 μm emission from Ho3+ ions. These results manifest that Tm3+/Ho3+ co-doped gallium tellurite glass is suitable for potential applications of broadband light sources and tunable fiber lasers operating in eye-safe 2.0 µm spectral region.
Mechanical Behavior of Al–Al2Cu–Si and Al–Al2Cu Eutectic Alloys
Authors: Lei Qian , Wang Jian , Misra Amit
Published: February 2021

In this study, laser rapid solidification technique was used to refine the microstructure of ternary Al–Cu–Si and binary Al–Cu eutectic alloys to nanoscales. Micropillar compression testing was performed to measure the stress–strain response of the samples with characteristic microstructure in the melt pool regions. The laser-remelted Al–Al2Cu–Si ternary alloy was observed to reach the compressive strength of 1.59 GPa before failure at a strain of 28.5%, which is significantly better than the as-cast alloy with a maximum strength of 0.48 GPa at a failure strain of 4.8%. The laser-remelted Al–Cu binary alloy was observed to reach the compressive strength of 2.07 GPa before failure at a strain of 26.5%, which is significantly better than the as-cast alloy with maximum strength of 0.74 GPa at a failure strain of 3.3%. The enhanced compressive strength and improved compressive plasticity were interpreted in terms of microstructural refinement and hierarchical eutectic morphology.
High-to-Low (Regional) Fertility Transitions in a Peripheral European Country: The Contribution of Exploratory Time Series Analysis
Authors: Rodrigo-Comino Jesus , Egidi Gianluca , Salvati Luca , Quaranta Giovanni , Salvia Rosanna , Gimenez-Morera Antonio
Published: February 2021

Diachronic variations in demographic rates have frequently reflected social transformations and a (more or less evident) impact of sequential economic downturns. By assessing changes over time in Total Fertility Rate (TFR) at the regional scale in Italy, our study investigates the long-term transition (1952–2019) characteristic of Mediterranean fertility, showing a continuous decline of births since the late 1970s and marked disparities between high- and low-fertility regions along the latitude gradient. Together with a rapid decline in the country TFR, the spatiotemporal evolution of regional fertility in Italy—illustrated through an exploratory time series statistical approach—outlines the marked divide between (wealthier) Northern regions and (economically disadvantaged) Southern regions. Non-linear fertility trends and increasing spatial heterogeneity in more recent times indicate the role of individual behaviors leveraging a generalized decline in marriage and childbearing propensity. Assuming differential responses of regional fertility to changing socioeconomic contexts, these trends are more evident in Southern Italy than in Northern Italy. Reasons at the base of such fertility patterns were extensively discussed focusing—among others—on the distinctive contribution of internal and international migrations to regional fertility rates. Based on these findings, Southern Italy, an economically disadvantaged, peripheral region in Mediterranean Europe, is taken as a paradigmatic case of demographic shrinkage—whose causes and consequences can be generalized to wider contexts in (and outside) Europe.
Role of Endoscopic Ultrasound in the Diagnosis of Pancreatic Neuroendocrine Neoplasms
Authors: Ishii Tatsuya , Katanuma Akio , Toyonaga Haruka , Chikugo Koki , Nasuno Hiroshi , Kin Toshifumi , Hayashi Tsuyoshi , Takahashi Kuniyuki
Published: February 2021

Although pancreatic neuroendocrine neoplasms (PNENs) are relatively rare tumors, their number is increasing with advances in diagnostic imaging modalities. Even small lesions that are difficult to detect using computed tomography or magnetic resonance imaging can now be detected with endoscopic ultrasound (EUS). Contrast-enhanced EUS is useful, and not only diagnosis but also malignancy detection has become possible by evaluating the vascularity of tumors. Pathological diagnosis using EUS with fine-needle aspiration (EUS-FNA) is useful when diagnostic imaging is difficult. EUS-FNA can also be used to evaluate the grade of malignancy. Pooling the data of the studies that compared the PNENs grading between EUS-FNA samples and surgical specimens showed a concordance rate of 77.5% (κ-statistic = 0.65, 95% confidence interval = 0.59–0.71, p < 0.01). Stratified analysis for small tumor size (2 cm) showed that the concordance rate was 84.5% and the kappa correlation index was 0.59 (95% confidence interval = 0.43–0.74, p < 0.01). The evolution of ultrasound imaging technologies such as contrast-enhanced and elastography and the artificial intelligence that analyzes them, the evolution of needles, and genetic analysis, will further develop the diagnosis and treatment of PNENs in the future.
Circulating miRNAs Are Associated with the Systemic Extent of Atherosclerosis: Novel Observations for miR-27b and miR-146
Authors: Pereira-da-Silva Tiago , Napoleão Patrícia , Costa Marina C. , Gabriel André F. , Selas Mafalda , Silva Filipa , Enguita Francisco J. , Ferreira Rui Cruz , Carmo Miguel Mota
Published: February 2021

The mechanisms that regulate the systemic extent of atherosclerosis are not fully understood. We investigated whether the expression of circulating miRNAs is associated with the extent of stable atherosclerosis to a single territory or multiple territories (polyvascular) and with the severity of atherosclerosis in each territory. Ninety-four participants were prospectively recruited and divided into five age- and sex-matched groups: presenting no atherosclerosis, isolated coronary atherosclerosis, coronary and lower extremity atherosclerosis, coronary and carotid atherosclerosis, and atherosclerosis of the coronary, lower extremity, and carotid territories. The expression of six circulating miRNAs with distinct biological roles was assessed. The expression of miR-27b and miR-146 differed across groups (p < 0.05), showing a decrease in the presence of atherosclerosis, particularly in the three territories. miR-27b and miR-146 expression decreased in association with a higher severity of coronary, lower extremity, and carotid atherosclerosis. Polyvascular atherosclerosis involving the three territories was independently associated with a decreased miR-27b and miR-146 expression. Both miRNAs presented an area under the curve of ≥0.75 for predicting polyvascular atherosclerosis involving the three territories. To conclude, miR-27b and miR-146 were associated with the presence of severe polyvascular atherosclerosis and with the atherosclerosis severity in each territory. Both are potential biomarkers of severe systemic atherosclerosis.
Beyond the Limits of Shannon’s Information in Quantum Key Distribution
Authors: Lizama-Pérez Luis Adrián , López R. J. Mauricio , Samperio Emmanuel H.
Published: February 2021

We present a new post-processing method for Quantum Key Distribution (QKD) that raises cubically the secret key rate in the number of double matching detection events. In Shannon’s communication model, information is prepared at Alice’s side, and it is then intended to pass it over a noisy channel. In our approach, secret bits do not rely in Alice’s transmitted quantum bits but in Bob’s basis measurement choices. Therefore, measured bits are publicly revealed, while bases selections remain secret. Our method implements sifting, reconciliation, and amplification in a unique process, and it just requires a round iteration; no redundancy bits are sent, and there is no limit in the correctable error percentage. Moreover, this method can be implemented as a post-processing software into QKD technologies already in use.
A Novel Solution Methodology Based on a Modified Gradient-Based Optimizer for Parameter Estimation of Photovoltaic Models
Authors: Hassan Mohamed H. , Kamel Salah , El-Dabah M. A. , Rezk Hegazy
Published: February 2021

In this paper, a modified version of a recent optimization algorithm called gradient-based optimizer (GBO) is proposed with the aim of improving its performance. Both the original gradient-based optimizer and the modified version, MGBO, are utilized for estimating the parameters of Photovoltaic models. The MGBO has the advantages of accelerated convergence rate as well as avoiding the local optima. These features make it compatible for investigating its performance in one of the nonlinear optimization problems like Photovoltaic model parameters estimation. The MGBO is used for the identification of parameters of different Photovoltaic models; single-diode, double-diode, and PV module. To obtain a generic Photovoltaic model, it is required to fit the experimentally obtained data. During the optimization process, the unknown parameters of the PV model are used as a decision variable whereas the root means squared error between the measured and estimated data is used as a cost function. The results verified the fast conversion rate and precision of the MGBO over other recently reported algorithms in solving the studied optimization problem.
Determination of Price Zones during Transition from Uniform to Zonal Electricity Market: A Case Study for Turkey
Authors: Poyrazoglu Gokturk
Published: February 2021

In the electricity market, different pricing models can be applied to increase market competitiveness. Different electricity systems use different market structures. Uniform marginal pricing, zonal marginal pricing, and nodal marginal pricing methods are commonly used market structures. For markets wishing to move from a uniform pricing structure to a more competitive zonal pricing structure, the determination of price zones is critical for achieving a competitive market that generates accurate price signals. Three different pricing zone detection algorithms are analyzed in this paper including the k-means clustering and queen/rook spatially constraint clustering. Finally, the results of a case study for the Turkish electricity system are shared to compare each method.
Day-Ahead Energy and Reserve Dispatch Problem under Non-Probabilistic Uncertainty
Authors: Shariatmadar Keivan , Arrigo Adriano , Vallée François , Hallez Hans , Vandevelde Lieven , Moens David
Published: February 2021

The current energy transition and the underlying growth in variable and uncertain renewable-based energy generation challenge the proper operation of power systems. Classical probabilistic uncertainty models, e.g., stochastic programming or robust optimisation, have been used widely to solve problems such as the day-ahead energy and reserve dispatch problem to enhance the day-ahead decisions with a probabilistic insight of renewable energy generation in real-time. By doing so, the scheduling of the power system becomes, production and consumption of electric power, more reliable (i.e., more robust because of potential deviations) while minimising the social costs given potential balancing actions. Nevertheless, these classical models are not valid when the uncertainty is imprecise, meaning that the system operator may not rely on a unique distribution function to describe the uncertainty. Given the Distributionally Robust Optimisation method, our approach can be implemented for any non-probabilistic, e.g., interval models rather than only sets of distribution functions (ambiguity set of probability distributions). In this paper, the aim is to apply two advanced non-probabilistic uncertainty models: Interval and ϵ-contamination, where the imprecision and in-determinism in the uncertainty (uncertain parameters) are considered. We propose two kinds of theoretical solutions under two decision criteria—Maximinity and Maximality. For an illustration of our solutions, we apply our proposed approach to a case study inspired by the 24-node IEEE reliability test system.
State of Art of Alkaline Earth Metal Oxides Catalysts Used in the Transesterification of Oils for Biodiesel Production
Authors: Tavizón-Pozos Jesús Andrés , Chavez-Esquivel Gerardo , Suárez-Toriello Víctor Alejandro , Santolalla-Vargas Carlos Eduardo , Luévano-Rivas Oscar Abel , Valdés-Martínez Omar Uriel , Talavera-López Alfonso , Rodriguez Jose Antonio
Published: February 2021

Biodiesel produced through catalytic transesterification of triglycerides from edible and non-edible oils and alcohol is considered an alternative to traditional petro-diesel. The interest in the use of alkaline earth metal oxides as heterogeneous basic catalysts has increased due to their availability, non-toxicity, the capacity to be reused, low cost, and high concentration of surface basic sites that provide the activity. This work is a compilation of the strategies to understand the effect of the source, synthesis, and thermal treatment of MgO, CaO, SrO, and BaO on the improvement of the surface basic sites density and strength, the morphology of the solid structure, stability during reaction and reusability. These parameters are commonly modified or enhanced by mixing these oxides or with alkaline metals. Also, the improvement of the acid-base properties and to avoid the lixiviation of catalysts can be achieved by supporting the alkaline earth metal oxides on another oxide. Additionally, the effect of the most relevant operation conditions in oil transesterification reactions such as methanol to oil ratio, temperature, agitation method, pressure, and catalysts concentration are reviewed. This review attempts to elucidate the optimum parameters of reaction and their application in different oils.
The Influence of Geometric Parameters of Pump Installation on the Hydraulic Performance of a Prefabricated Pumping Station
Authors: Zhang Bowen , Cheng Li , Xu Chunlei , Wang Mo
Published: February 2021

A prefabricated pumping station is a new type of pumping station that plays an important role in the construction of sponge cities in developing countries. It solves the problem of urban water-logging and makes great contributions to the sustainable development of water resources. In order to research the influence of different installation positions of pumps on the internal hydraulic performance of a prefabricated pumping station, based on ANSYS software, the computational fluid dynamics (CFD) numerical simulation method was used to analyze the internal flow state of the prefabricated pump station. In this research, the optimal geometric parameters of pump installation in a prefabricated pumping station are given. The results show that when the distance between the connecting line of two pumps and the center of the sump is L = 0.2 R, the distance between the two pumps is S = 0.6 R, and the suspension height of the two pumps is H = 0.6 D, the internal flow pattern of the prefabricated pump station is better. (R is the cross-sectional radius of the sump and D is the diameter of the nozzle of the pump horn.) These research results have certain guiding significance for improvement of the hydraulic performance and operation efficiency of prefabricated pump stations. They also provide a theoretical basis for parameter selection for prefabricated pumping stations.
Effects of Thinning Practice, High Pruning and Slash Management on Crop Tree and Stand Growth in Young Even-Aged Stands of Planted Silver Birch (Betula pendula Roth)
Authors: Skovsgaard Jens Peter , Johansson Ulf , Holmström Emma , Tune Rebecka McCarthy , Ols Clémentine , Attocchi Giulia
Published: February 2021

The objective was to quantify the influence of thinning, high pruning and slash management on crop tree and stand growth in young even-aged stands of planted silver birch (Betula pendula Roth). This study was based on two field experiments, aged six and eleven years at initiation and re-measured after six and eight years, respectively. Treatments included the unthinned control, moderate thinning mainly from below (removing 28–33% of standing volume), point thinning to favor 300 trees per ha and with no thinning elsewhere in the plot (removing 16–25%), and heavy thinning leaving 600 evenly distributed potential future crop trees per ha (removing 64–75%). Slash management (extraction or retention) was applied to heavily thinned plots. High pruning removing 30–70% of the green crown was carried out in some plots with point or heavy thinning on 300 or 600 trees per ha, respectively. Stand volume growth increased with increasing pre-treatment mean annual volume increment and decreased with increasing thinning intensity as compared to the unthinned control. LS-means estimates indicated a reduction for moderate thinning by 14%, for point thinning by 12% and for heavy thinning (combined with pruning) by 62%. However, in the youngest experiment, heavy thinning (without pruning) reduced growth by 54%. Combining these results with results from a similar experiment in Canada, the reduction in stand volume growth (RedIv%) depending on thinning removal (RemV%), both expressed as a percentage of the unthinned control, was quantified as RedIv% = −23.67 + 1.16·RemV% (calibration range: 30–83%). For heavy thinning (large quantities of slash), slash extraction resulted in no reduction in stand volume growth as compared to slash retention. The instantaneous numeric reduction in the average stem diameter of the 300 thickest trees per ha (D300) due to thinning was 3.5, 15–21% and 955–11% with moderate, point and heavy thinning, respectively. The subsequent average annual increase in D300 during the observation period was 8.5%, 25 and 18%, respectively. In the youngest experiment, pruning in unthinned plots led to a reduction in the annual increase of D300 by 14%, and heavy thinning in unpruned plots led to an increase by 30%. The growth of pre-selected potential future crop trees increased with increasing thinning intensity. In heavily thinned plots, pruning reduced growth increasingly with increasing pruning severity; LS-means estimates indicated 21% larger growth on stem diameter for unpruned trees and 3% for pruned trees. As an adverse side effect, heavily thinned plots with only 600 trees per ha were at increased risk of windthrow for some years after the thinning intervention. In the oldest experiment, 95–21% of the trees in these plots were damaged by wind.