bluuebunny/crossref_metadata_2025 huggingface dataset:
UMA IDEIA DE ÉTICA E EDUCAÇÃO DIANTE DAS NOVAS TECNOLOGIAS
Authors: Stroebele Agda
Published: August 2024

Este estudo investiga a relevância do ensino da ética na educação básica diante das transformações sociais e tecnológicas contemporâneas. A partir da compreensão dos conceitos de axiologia e valores, e de suas implicações nas relações sociais e morais, o trabalho destaca a importância de uma formação que integra reflexão ética e prática cidadã. Entende-se que a escola deve promover não apenas a transmissão de conhecimentos, mas também a formação de cidadãos críticos e responsáveis. A investigação baseia-se em referências filosóficas e educacionais, destacando a necessidade de uma educação que contemple conteúdos conceituais, procedimentais e atitudinais. Conclui-se que a educação ética é essencial para a construção de uma sociedade mais justa e democrática, preparando os indivíduos para agir eticamente em um mundo em constante transformação.
POLIPOSE INTESTINAL NA SÍNDROME DE PEUTZ-JEGHERS: UMA REVISÃO ABRANGENTE DE DIAGNÓSTICO E TRATAMENTO
Authors: Oliveira Aline Boechat de , Mendes Aline Gabrielle de Oliveira , Castro Ana Beatriz Oliveira , Prado Angélica Adriene Thomaz de Souza , Costa Larissa de Oliveira Velloso , Morais ⁠Pedro Henrique Rivas de , Santos Weskley Souza dos
Published: August 2024

A síndrome de Peutz-Jeghers (SPJ) é uma condição genética rara caracterizada por pólipos hamartomatosos no trato gastrointestinal e hiperpigmentação mucocutânea, associada a um risco elevado de malignidades. Este artigo de revisão tem como objetivo analisar as manifestações clínicas, diagnósticos e abordagens terapêuticas da SPJ, destacando os avanços no manejo e as áreas que necessitam de mais pesquisas. A metodologia utilizada foi uma revisão narrativa da literatura, abrangendo artigos publicados entre 2015 e 2024 nas bases de dados PubMed, Scopus e Web of Science. Foram selecionados estudos que abordam aspectos clínicos, genéticos e terapêuticos da síndrome. Os resultados indicam que o manejo dos pólipos através de remoção endoscópica e vigilância para detecção precoce de cânceres são fundamentais para melhorar o prognóstico. A discussão revelou que, apesar dos avanços no diagnóstico genético e nas técnicas endoscópicas, desafios persistem, especialmente em termos de acesso a cuidados de saúde adequados e intervenções psicossociais. Conclui-se que a SPJ requer uma abordagem multidisciplinar que combine vigilância clínica rigorosa, tratamento eficaz e suporte psicológico, com o objetivo de melhorar a qualidade de vida dos pacientes e reduzir a mortalidade associada.
Developmental Psychology In the Arabic Language Learning and Teaching Process/ Psikologi Perkembangan Dalam Proses Belajar Mengajar Bahasa Arab
Authors: Nuzula Firdausi , Mufidah Nuril
Published: August 2024

This article discusses the importance of developmental psychology in learning and teaching the Arabic language. Data were obtained from a literature study in the form of journal article documents and reference books related to developmental psychology and Arabic language learning. The results of the study showed that developmental psychology provides insights into how individuals develop cognitively, socially, and emotionally at various stages of their lives. This understanding is crucial for teachers in designing and implementing effective teaching strategies that cater to the needs of students. The article highlights how teaching strategies tailored to students' developmental stages can enhance motivation and learning outcomes. Additionally, the article identifies the challenges teachers face in applying the principles of developmental psychology, such as individual differences among students, limited resources, and inadequate training. Teachers can create a more inclusive and effective learning environment by addressing these challenges through continuous support and training. Implementing developmental psychology in Arabic language teaching is expected to help students reach their full potential and improve the overall quality of education.
Optimization Of Audio Visual Media To Improve Students' Concentration In Arabic Language Learning/ Optimalisasi Media Audio Visual Untuk Meningkatkan Konsentrasi Siswa Pada Pembelajaran Bahasa Arab
Authors: Hotimah Khusnul , Noor Fatwiah
Published: August 2024

This study describes the optimization of audiovisual media to increase student concentration in Arabic language learning at MI Azharul Ulum 02 Malang. This study aims to describe the nature of audiovisual learning media and its varieties, the causes of decreased concentration in students, and the implementation of learning media using audiovisuals to overcome decreased concentration in students. This study uses a qualitative descriptive method of case study type. The data collection technique is observation and data techniques using observations at MI AZHARUL ULUM 02, totaling 35 students in 2024, and documentation. This article uses the Miles, M.B. and Huberman, A.M. model for data analysis. The study results showed that audiovisuals are divided into active and non-active. Audiovisual media to improve student concentration can train istima' and qiroah simultaneously, explain learning clearly, save time and costs, help educators deliver learning practically, and help students remember learning more. Two factors can affect student concentration: internal factors (assumptions, willingness or interest, and physical condition) and external factors (lack of support, environmental influences, and monotonous learning presentation models). As for the form of audiovisual implementation in Arabic language learning, researchers raised several material titles. Such as التّعارف, المهنة, اللّون, عدد, الأسرة. Audiovisuals are cartoon and animation videos that display educational videos and songs. After that, the educator guides learning by giving assignments to make videos, singing songs related to the material, and guiding children's understanding through brief explanations. Using audiovisual learning media to improve student concentration is effective for educators.
Analisis Keputusan Pembelian Online Pada E-Commerce Shopee Ditinjau Dari Privasi, Keamanan Dan Kepercayaan
Authors: Fitrianingsih Fitrianingsih , Fitriani Hilda
Published: August 2024

The aim of this research is to determine the influence of privacy, security and trust on online purchasing decisions on Shopee E-Commerce among the people of Pematang Kuing Village, Sei Suka District, Batu Bara Regency. The population in this research is all Shopee consumers who live in Pematang Kuing Village, Sei Suka District, Batu Bara Regency with a sample of 43 respondents. Hypothesis testing uses multiple regression analysis on the SPSS version 25 application. The results of this research show that partially privacy does not have a significant effect on the purchasing decisions of the people of Pematang Kuing Village, Sei Suka District, Batu Bara Regency. Meanwhile, security and trust have a significant influence on the purchasing decisions of the people of Pematang Kuing Village, Sei Suka District, Batu Bara Regency. Based on a simultaneous test, privacy, security and trust together (simultaneously) have a significant effect on the people of Pematang Kuing Village, Sei Suka District, Batu Bara Regency.
Pengaruh Flash Sale Dan Promo Gratis Ongkos Kirim Terhadap Keputusan Pembelian Pada E-Commerce Shopee
Authors: Hutagalung Hermanto , Anggraini Gea
Published: August 2024

The e-commerce business is growing and developing rapidly in Indonesia, the high interest in online shopping in the community has made a lot of new e-commerce pop up in Indonesia. One of the largest e-commerce providers in Indonesia is Shopee. Flash sale programs and free shipping promos make Shopee one of the most popular online platforms in Indonesia. This study aims to analyze how the effect of flash sale and free shipping promos on purchasing decisions of e-commerce Shopee. This type of research is quantitative research with an associative approach. Sampling used a purposive sampling technique and use 65 respondents as samples. The primary data used in this study was obtained by distributing questionnaires via google form, while the secondary data were obtained through a literature study. The data analysis method used the validity test, reliability test, classical assumption test, multiple linear regression test, and hypothesis test. The results of the research conducted showed that the flash sale variable (X1) did not significantly influence purchasing decisions. Meanwhile, the variable free shipping promo (X2) has a significant effect on purchasing decisions, however, the flash sale and free shipping promo variables simultaneously (simultaneously) affect purchasing decisions (Y). The coefficient of determination test shows that there is a fairly close relationship between flash sales and free shipping promos on purchasing decisions with an R-value of 0,836. Through the adjusted R square value, it is also known that the flash sale variable and free shipping promo contributed 69% to the purchasing decision variable, while the remaining 31% was explained by other variables outside this research model.
Pengaruh Kualitas Produk Dan Label Halal Terhadap Keputusan Pembelian Dengan Kepercayaan Sebagai Variabel Mediasi
Authors: Manurung Carnelius Ricardo , Rosida Rosida , Sarwoto Sarwoto
Published: August 2024

The aim of this study was to examine how the impact of product quality and the presence of a halal label affect consumer purchasing choices, with trust playing a mediating role. The study focused on Scarlett's Instagram followers. The study explored both direct and indirect effects. This research falls under the category of explanatory research, aiming to elucidate causal relationships between variables through hypothesis testing. The study employed a quantitative approach utilizing SmartPLS (Partial Least Square) software version 3.0. Two sub-models were utilized: the measurement model (outer model) and the structural model (inner model). The sampling method employed was accidental, with 97 respondents chosen using the Cochran formula. The direct impact analysis revealed that product quality significantly influenced purchasing decisions, with a value of 2.246 and a significant value of 0.025. However, the presence of a halal label did not significantly affect purchasing decisions, registering a value of 0.317 and a significant value of 0.751. Product quality significantly influenced trust, with a value of 7.556 and a significant value of 0.000, while the halal label did not significantly affect trust, with a value of 1.934 and a significant value of 0.054. Trust significantly influenced purchasing decisions, with a value of 20.906 and a significant value of 0.000. Regarding the indirect effects, trust was found to mediate the relationship between product quality and purchasing decisions, with a value of 6.618 and a significant value of 0.000. However, trust was not found to mediate the relationship between halal labels and purchasing decisions, with a value of 1.943 and a significant value of 0.053.
Evolução e Abordagens Contemporâneas no Diagnóstico e Tratamento de Fraturas Mandibulares: Uma Revisão de Literatura
Authors: Ribeiro Narciso Laís , Jesus Bárbara Mendes de , Silva Artur Moreira da , Silva Edwin Renylson Carvalho da , Valim Olavo Quintino Candido Mariotini , Silva Cássia Reis da , Fernandes Tândara Pereira , Nulli Vittorio Hugo Oliveira Moscetti , Ott Thiely Rodrigues , Sorio Penélope Danieli , Santos Gabriella Maria Belarmino dos , Silva Maria Gabrielly Costa e , Aguero Pedro dos Santos Anjo e , Lima Kleber Meireles de , Justa Jose William Oliveira dos Santos
Published: August 2024

O objetivo desta revisão foi examinar as abordagens clínicas e os desafios no tratamento das fraturas mandibulares, focando nas técnicas atuais, avanços tecnológicos e áreas para futuras pesquisas. A revisão sistemática da literatura foi realizada na plataforma PubMed, selecionando 25 artigos relevantes dos últimos dez anos. A pesquisa abordou técnicas cirúrgicas, inovações tecnológicas e estratégias de reabilitação para fraturas mandibulares, incluindo o uso de tecnologias como tomografia computadorizada e modelagem 3D, além de destacar a importância de uma abordagem multidisciplinar. Os resultados evidenciam uma ampla gama de técnicas para o tratamento de fraturas mandibulares, desde métodos conservadores até técnicas cirúrgicas avançadas. O uso de tecnologias como a tomografia computadorizada de feixe cônico (CBCT) e a realidade aumentada tem melhorado a precisão no diagnóstico e planejamento cirúrgico. A pesquisa também sublinha a importância da integração de tecnologias avançadas e a colaboração entre cirurgiões, dentistas e fisioterapeutas para um tratamento eficaz e uma recuperação otimizada. Entre os desafios identificados estão a complexidade das fraturas e a necessidade de coordenação entre diferentes especialidades médicas. A revisão aponta para a eficácia das técnicas cirúrgicas contemporâneas e das tecnologias emergentes no manejo das fraturas mandibulares, mas também destaca a necessidade de mais pesquisas para desenvolver protocolos integrados e evidências robustas. O avanço contínuo das tecnologias e a abordagem multidisciplinar são essenciais para melhorar os resultados clínicos e a qualidade de vida dos pacientes. Recomenda-se a realização de estudos futuros para explorar novas tecnologias e abordagens terapêuticas, com o objetivo de otimizar o manejo das fraturas mandibulares e enfrentar os desafios associados a essas lesões complexas.
PERAN HRD SEBAGAI PILAR PELATIHAN DAN PEGAWASAN KINERJA SDM PADA HOTEL WP
Authors: Vidia Ariadna Tommy Ibrahim  , Made Iska Aprilita Wardani
Published: August 2024

Hotel WP salah satu usaha bidang hospitality dengan sistem pemasaran, reservasi, dan sistem operasional yang profesional. Penelitian ini bertujuan untuk mengetahui fungsi HRD pada kegiatan pelatihan dan mengawasi kinerja SDM di hotel WP. Metode kualitatif yaitu mengamati peran HRD dalam pelatihan dan pengawasan kinerja SDM termasuk Standart Operasional Prosedur (SOP), standart kinerja, dan disiplin kerja sebagai keberhasilan kinerja. Pemilihan obyek penelitian karena usaha banyak customer order namun masih membutuhkan perhatian terhadap Peranan HRD sebagai backup utama pelatihan dan pengawasan kinerja SDM.
Peran HRD dalam pelatihan yaitu perencanaan, penjadwalan, pelaksanaan serta analisa dan evaluasi pelatihan terhadap karyawan. Dalam pengawasan, HRD memenuhi Standart Operasional Prosedur (SOP) yang dijalankan dan memberi hasil kinerja lebih baik sesuai tujuan. Pengawasan secara continuous improvement dapat mengendalikan kinerja dan meminimalisir kesalahan supaya lebih efisien.
Kata kunci : pelatihan, pengawasan, hotel, kinerja, efisiensi
PENGARUH HARGA, KUALITAS PRODUK, DAN KUALITAS PELAYANAN TERHADAP KEPUASAN KONSUMEN PADA FCK DIGITAL PRINTING SOLUTION MALANG
Authors: Niajeng Putri Ulfa Hakim  , Anis Dwiastanti  , Roni Angger Aditama
Published: August 2024

The purpose of this study seeks to analyze Price, Product Quality, and Service Quality both partially and simultaneously on Consumer Satisfaction and analyze the dominant influence between Price, Product Quality, and Service Quality on Consumer Satisfaction. The population in this study is all consumers of FCK Digital Printing Solution Malang. While the sample was taken using a problability sampling technique with random sampling and obtained a sample of 96 respondents.
The examination utilized in this study was various direct relapse investigation. That's what the outcomes showed (1) to some extent the Cost variable affected Shopper Fulfillment, (2) to some degree the Item Quality variable doesn't influence Customer Fulfillment, (3) somewhat the variable Help Quality affects Buyer Fulfillment, (4) at the same time there is a huge impact between Value, Item Quality, and Administration Quality on Purchaser Fulfillment. The extent of impact was 53.1%, while the excess 46.9% was affected by different factors that were excluded from this review, (5) the Assistance Quality variable had the best effect on Consumer loyalty.
 Keywords: Price, Product Quality, Service Quality, and Customer Satisfaction
AUDIT INVESTIGASI ATAS SIKLUS PENGELUARAN DAN PEMASUKAN PT S (Studi Kasus pada Klien KAP Gideon Adi dan Rekan)
Authors: Herwinda Anggun  , Budiwitjaksono Gideon
Published: August 2024

Audit investigasi merupakan audit yang dilakukan untuk membantu mengungkap adanya kesalahan dan kejahatan yang dapat terjadi terhadap hasil laporan keuangan. Terdapat indikasi fraud yang dilakukan oleh beberapa pihak dari PT S, sehingga dilakukan audit investigasi untuk mengungkap apakah benar telah terjadi fraud pada PT S. Tujuan penelitian ini dengan maksud untuk mengetahui prosedur yang dilakukan KAP Gideon Adi dan Rekan dalam menjalankan audit investigasi serta untuk mengetahui langkah yang dapat diambil untuk melakukan pencegahan atas permasalahan tersebut. Penelitian ini menggunakan data primer sebagai sumber utama. Data primer didapat melalui wawancara dengan pihak KAP. Sedangkan metode pengumpulan data yang digunakan adalah metode dokumentasi. Dokumen yang menjadi sumber data penulis adalah laporan keuangan PT S. penelitian ini menggunakan metode analisis data yaitu metode deskriptif kualitatif. Setelah dilakukannya pengujian terhadap keseluruhan rekening koran dan ditemukan adanya fraud dan pihak-pihak yang bertanggung jawab telah diidentifikasi. Proses audit investigasi yang dilakukan oleh auditor pada PT S dilakukan sesuai dengan prosedur Standar Audit dan Standar Profesional Akuntan Publik. Prosedur ini menunjukkan jenis kecurangan, faktor-faktor yang menyebabkan kecurangan, serta metode untuk menangani kecurangan.
 
Kata kunci: Audit investigasi, Fraud
PENGARUH CITRA MEREK TERHADAP MINAT MASYARAKAT UNTUK MEMBELI PRODUK TABUNGAN SYARIAH DI BTM DINAR NASYIAH 01 BOJONEGORO
Authors: Emiarti Endah
Published: August 2024

This research aims to determine the partial and determinative influence of brand image on community interest in saving with Sharia savings products at KSPPS BTM Dinar Nasyiah 01 Bojonegoro. The population of this study were all participant of BTM Dinar Nasyiah 01. The sample in this study was 100 respondents, determined using purposive sampling technique. The data used were primary data obtained from respondents' answers through distributed questionnaires. Data processing was carried out using SPSS (Statistical Package for Social Science) with simple linear analysis method. The results of this study indicate that brand image has a positive and significant influence on community interest. The management should focus more on branding the image of the syariah saving product so that can increase the sales.
Implementing the Humanistic Approach in 21st-Century Interactive Arabic Language Learning at Madrasah Ibtidaiyah
Authors: Lestari Yuni , Yusuf Yusuf Muhtarom , Maksudin Maksudin
Published: August 2024

The purpose of this study is to elucidate the paradigm of the humanistic approach in 21st-century interactive Arabic language instruction at Madrasah Ibtidaiyah. This research employs a library research methodology, sourcing data from literature, various websites, journals, dictionaries, encyclopedias, documents, and magazines. The findings demonstrate that the humanistic approach is instrumental in helping students comprehend Arabic by integrating contemporary digital learning developments. This approach focuses on nurturing students' cognitive, affective, and psychomotor skills through a supportive and engaging educational environment. The study highlights the necessity of this approach in overcoming the challenges students face in learning Arabic, ensuring a more enjoyable and less daunting learning experience. The humanistic approach serves as a strategic framework to facilitate effective Arabic language acquisition, aligned with the demands of 21st-century education.
The Utilization of Picture Puzzle Media to Enhance Arabic Vocabulary Mastery at Madrasah Ibtidaiyah Bandar Lampung
Authors: Saputra Dani , Syahril Sulthan , Irwandani Irwandani
Published: August 2024

This study aims to implement and explore the use of pictorial puzzle media in enhancing students' Arabic vocabulary mastery. The method used in this study is Classroom Action Research (CAR), conducted in 2 cycles, with each stage consisting of four steps: planning, implementation, observation, and reflection. The research instruments used were student observation sheets and test results. From the preliminary study conducted on 13 students, only 3 students achieved the minimum mastery criteria, while 10 students did not, with an average score of only 69.7, whereas the minimum mastery criteria at Madrasah Nurul Hikmah is 75, indicating that the students' scores were still below the minimum mastery criteria. The research results after implementing pictorial puzzle media showed an increase in the Arabic vocabulary mastery of class V students at Madrasah Nurul Hikmah Bandar Lampung. This was evidenced by the increase in the average scores of students, which rose from 69.7 in the pre-cycle to 74.6 in cycle I and 88.5 in cycle II. The regular use of pictorial puzzle media can enhance students' ability to master Arabic vocabulary. This indicates that this method can be an effective solution for improving Arabic language learning in Madrasahs, and provides evidence that using visual approaches can greatly aid in improving students' learning outcomes.
A comparative study of the cryo-EM structures of S. cerevisiae and human anaphase-promoting complex/cyclosome (APC/C)
Authors: Vazquez-Fernandez Ester , Yang Jing , Zhang Ziguo , Andreeva Antonina E , Emsley Paul , Barford David
Published: August 2024

The anaphase-promoting complex/cyclosome (APC/C) is a large multi-subunit E3 ubiquitin ligase that controls progression through the cell cycle by orchestrating the timely proteolysis of mitotic cyclins and other cell cycle regulatory proteins. Although structures of multiple human APC/C complexes have been extensively studied over the past decade, the
S. cerevisiae
APC/C has been less extensively investigated. Here, we describe medium resolution structures of three
S. cerevisiae
APC/C complexes: unphosphorylated apo-APC/C and the ternary APC/C
CDH1
-substrate complex, and phosphorylated apo-APC/C. Whereas the overall architectures of human and
S. cerevisiae
APC/C are conserved, as well as the mechanism of CDH1 inhibition by CDK-phosphorylation, specific variations exist, including striking differences in the mechanism of coactivator-mediated stimulation of E2 binding, and the activation of APC/C
CDC20
by phosphorylation. In contrast to human APC/C in which coactivator induces a conformational change of the catalytic module APC2:APC11 to allow E2 binding, in
S. cerevisiae
apo-APC/C the catalytic module is already positioned to bind E2.Furthermore, we find no evidence of a phospho-regulatable auto-inhibitory segment of APC1, that in the unphosphorylated human APC/C, sterically blocks the CDC20
C-box
binding site of APC8. Thus, although the functions of APC/C are conserved from
S. cerevisiae
to humans, molecular details relating to their regulatory mechanisms differ.
Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking
Authors: Wong Victor C , Houlihan Patrick R , Liu Hui , Walpita Deepika , DeSantis Michael C , Liu Zhe , O'Shea Erin K
Published: August 2024

AMPA-type receptors (AMPARs) are rapidly inserted into synapses undergoing plasticity to increase synaptic transmission, but it is not fully understood if and how AMPAR-containing vesicles are selectively trafficked to these synapses. Here, we developed a strategy to label AMPAR GluA1 subunits expressed from their endogenous loci in cultured rat hippocampal neurons and characterized the motion of GluA1-containing vesicles using single-particle tracking and mathematical modeling. We find that GluA1-containing vesicles are confined and concentrated near sites of stimulation-induced structural plasticity. We show that confinement is mediated by actin polymerization, which hinders the active transport of GluA1-containing vesicles along the length of the dendritic shaft by modulating the rheological properties of the cytoplasm. Actin polymerization also facilitates myosin-mediated transport of GluA1-containing vesicles to exocytic sites. We conclude that neurons utilize F-actin to increase vesicular GluA1 reservoirs and promote exocytosis proximal to the sites of synaptic activity.
A rapid phylogeny-based method for accurate community profiling of large-scale metabarcoding datasets
Authors: Pipes Lenore , Nielsen Rasmus
Published: August 2024

Environmental DNA (eDNA) is becoming an increasingly important tool in diverse scientific fields from ecological biomonitoring to wastewater surveillance of viruses. The fundamental challenge in eDNA analyses has been the bioinformatical assignment of reads to taxonomic groups. It has long been known that full probabilistic methods for phylogenetic assignment are preferable, but unfortunately, such methods are computationally intensive and are typically inapplicable to modern next-generation sequencing data. We present a fast approximate likelihood method for phylogenetic assignment of DNA sequences. Applying the new method to several mock communities and simulated datasets, we show that it identifies more reads at both high and low taxonomic levels more accurately than other leading methods. The advantage of the method is particularly apparent in the presence of polymorphisms and/or sequencing errors and when the true species is not represented in the reference database.
Why the brown ghost chirps at night
Authors: Oboti Livio , Pedraja Federico , Ritter Marie , Lohse Marlena , Klette Lennart , Krahe Rüdiger
Published: August 2024

Since the pioneering work by Moeller, Szabo, and Bullock, weakly electric fish have served as a valuable model for investigating spatial and social cognitive abilities in a vertebrate taxon usually less accessible than mammals or other terrestrial vertebrates. These fish, through their electric organ, generate low-intensity electric fields to navigate and interact with conspecifics, even in complete darkness. The brown ghost knifefish is one of the most widely studied species due to its rich electric vocabulary, made by individually variable and sex-specific electric signals. These are mainly characterized by brief frequency modulations of the oscillating dipole moment emitted continuously by their electric organ and are known as chirps. Different types of chirps are believed to convey specific and behaviorally salient information, serving as behavioral readouts for different internal states during behavioral observations. Despite the success of this model in neuroethology over the past seven decades, the code to decipher their electric communication remains unknown.This study re-evaluates this view, aiming to offer an alternative, and possibly complementary, explanation for why these freshwater bottom dwellers emit electric chirps. By uncovering correlations among chirping, electric field geometry, and detectability in enriched environments, we present evidence for a previously unexplored role of chirps as specialized self-directed signals, enhancing conspecific electrolocation during social encounters.
Expression of Most Retrotransposons in Human Blood Correlates with Biological Aging
Authors: Tsai Yi-Ting , Seymen Nogayhan , Thompson I Richard , Zou Xinchen , Mumtaz Warisha , Gerlevik Sila , Mufti Ghulam J , Karimi Mohammad M
Published: August 2024

Retrotransposons (RTEs) have been postulated to reactivate with age and contribute to aging through activated innate immune response and inflammation. Here, we analyzed the relationship between RTE expression and aging using published transcriptomic and methylomic datasets of human blood. Despite no observed correlation between RTEs activity and chronological age, the expression of most RTE classes and families except short interspersed nuclear elements (SINEs) correlated with biological age-associated gene signature scores. Strikingly, we found that the expression of SINEs was linked to upregulated DNA repair pathways in multiple cohorts. We also observed DNA hypomethylation with aging and significant increase in RTE expression level in hypomethylated RTEs except for SINEs. Additionally, our single-cell transcriptomic analysis suggested a role for plasma cells in aging mediated by RTEs. Altogether, our multi-omics analysis of large human cohorts highlights the role of RTEs in biological aging and suggests possible mechanisms and cell populations for future investigations.
Additional feedforward mechanism of Parkin activation via binding of phospho-UBL and RING0 in trans
Authors: Lenka Dipti , Dahe Shakti , Antico Odetta , Sahoo Pritiranjan , Prescott Alan R. , Muqit Miratul M. K. , Kumar Atul
Published: August 2024

Loss of function Parkin mutations lead to early-onset of Parkinson’s disease. Parkin is an auto-inhibited ubiquitin E3 ligase activated by dual phosphorylation of its ubiquitin-like (Ubl) domain and ubiquitin by the PINK1 kinase. Herein, we demonstrate a competitive binding of the phospho-Ubl and RING2 domains towards the RING0 domain, which regulates Parkin activity. We show that phosphorylated Parkin can complex with native Parkin, leading to the activation of autoinhibited native Parkin in trans. Furthermore, we show that the activator element (ACT) of Parkin is required to maintain the enzyme kinetics, and the removal of ACT slows the enzyme catalysis. We also demonstrate that ACT can activate Parkin in trans but less efficiently than when present in the cis molecule. Furthermore, the crystal structure reveals a donor ubiquitin binding pocket in the linker connecting REP and RING2, which plays a crucial role in Parkin activity.
AARS2 ameliorates myocardial ischemia via fine-tuning PKM2-mediated metabolism
Authors: Zhang Zongwang , Zheng Lixia , Chen Yang , Chen Yuanyuan , Hou Junjie , Xiao Chenglu , Zhu Xiaojun , Zhao Shi-Min , Xiong Jing-Wei
Published: August 2024

AARS2, an alanyl-tRNA synthase, is essential for protein translation, but its function in mouse hearts is not fully addressed. Here, we found that cardiomyocyte-specific deletion of mouse AARS2 exhibited evident cardiomyopathy with impaired cardiac function, notable cardiac fibrosis and cardiomyocyte apoptosis. Cardiomyocyte-specific AARS2 overexpression in mice improved cardiac function and reduced cardiac fibrosis after myocardial infarction (MI), without affecting cardiomyocyte proliferation and coronary angiogenesis. Mechanistically, AARS2 overexpression suppressed cardiomyocyte apoptosis and mitochondrial reactive oxide species production, and changed cellular metabolism from oxidative phosphorylation toward glycolysis in cardiomyocytes, thus leading to cardiomyocyte survival from ischemia and hypoxia stress. Ribo-Seq revealed that AARS2 overexpression increased pyruvate kinase M2 (PKM2) protein translation and the ratio of PKM2 dimers to tetramers that promote glycolysis. Additionally, PKM2 activator TEPP-46 reversed cardiomyocyte apoptosis and cardiac fibrosis caused by AARS2 deficiency. Thus, this study demonstrates that AARS2 plays an essential role in protecting cardiomyocytes from ischemic pressure via fine-tuning PKM2-mediated energy metabolism, and presents a novel cardiac protective AARS2-PKM2 signaling during the pathogenesis of MI.
Exploring the Impact of ESG Performance and R&D Capabilities on Corporate Financial Success in China
Authors: Linyu Fan
Published: August 2024

China has set the "dual carbon" goal of achieving "carbon neutrality" and "carbon peak" by 2024. Based on this background, this article explores the impact of ESG and R&D capabilities on corporate financial performance, and whether they have a driving effect on the "dual carbon" target. This study takes the non-equilibrium panel data of Chinese A-share listed companies from 2017 to 2022 as the research object, proposes research hypotheses, and constructs an empirical model. This article uses SPSS software to apply statistical description, multicollinearity test, least squares regression analysis and other empirical methods to the sample. The model is further tested through robustness test and heterogeneity test. The study shows that at the current stage, the ESG performance and innovation ability of enterprises have a positive impact on their financial performance. Based on this, the article also provides corresponding operational suggestions for enterprises.
Sustainable Tax Recovery to Prevent Tax Fraud in The Jabodetabek Area
Authors: Andaningsih IGP Ratih , Novita Novita , Nugrahanti Trinandari Prasetya , Arif Elsya Meida
Published: August 2024

In Conducting this field research,researchers conducted field research related to the practice of implementing tax and anti fraud (Tax Fraud) among MSMEs business players in Indonesia which refers to Law No 7 of 2021 in relation to economic recovery in the field of tax reform.The Aim to be achieved in this research to increase revenue .The research method used are used combinations a qualitative method and a quantitative method. A qualitative method used a symetric method with action research, critical analysis, quantitative narrative analysis, a Interviews and practical interview models and field observations with a symetric partial interest analysis approach using informant data. Representatives of business actors in the Jabodetabek area and surroundings areas, in Indonesia which has a gross turnover of 100 million per month. This research is very useful for increasing the potential and revenue of regional taxes and central taxes in the MSMe business distribution sector optimally and optimally. The contribution of the research is to increase tax revenues, especially the MSMe taxation sector. The results of the research are to increase the culture of awareness of paying taxes and be able to increase sources of state revenue.
The Influence of Thin Capitalization, Capital Intensity and Sales Growth on Tax Avoidance with Institutional Ownership as a Moderating Variable in Manufacturing Companies Registered as Indonesian Sharia Stock Index Companies
Authors: Ayuningtia Miranda , Pramiana Omi
Published: August 2024

This research aims to determine the effect of thin capitalization, capital intensity and sales growth on tax avoidance with institutional ownership as a moderating variable in manufacturing companies listed as Indonesian sharia stock index companies in 2021-2022. This research uses a quantitative descriptive method, with a total sample of 52 companies with 104 data used obtained from the company's annual financial reports. The results of this research show that thin capitalization, capital intensity, sales growth has an effect on tax avoidance, institutional ownership is able to moderate the effect of thin capitalization on tax avoidance, institutional ownership is able to moderate the effect of capital intensity on tax avoidance and institutional ownership unable to moderate the influence of sales growth on tax avoidance.
Early signatures of ozone trend reversal over the Antarctic
Authors: Várai Anita , Homonnai Viktória , Jánosi Imre M. , Müller Rolf
Published: February 2015

We report on a detailed time series analysis of long total column ozone (TO) records based on multi‐satellite observations of daily resolution. We concentrate on three geographic latitudes over and around the Antarctic area, specifically on three circles at 58.5°S, 59.5°S, and 79.5°S. Almost continuous observations are available at the two former latitudes; however, data are lacking during the polar winter periods at 79.5°S, because the measurement technique requires sunlight. The methodology is motivated by level‐crossing statistics, where subsets of the records above or below particular threshold levels are evaluated. Long‐term trend reversal at around the turn of the century is already detectable for low TO levels in the raw time series in the “ozone‐hole” region (79.5°S). In order to overcome the apparent non‐stationarities of the time series, we determined daily TO differences (ΔTO) belonging to the same geographic longitudes between the different latitudinal circles. The result is a stable, stationary behavior for small (absolute) ΔTO values in the period January–February–March without any significant detectable trends. The high absolute value ΔTO subsets (September–October–November) indicate a robust trend reversal in the middle of the 1990s. The observed trend reversal in the total column ozone time series is consistent with the temporal development of the stratospheric halogen loading. However, a close correspondence of ozone and halogen turnaround years is not expected because of the statistical uncertainties in the determination of the ozone turnaround, and the many factors contributing to ozone depletion processes.
An efficient and guaranteed stable numerical method for continuous modeling of infiltration and redistribution with a shallow dynamic water table
Authors: Lai Wencong , Ogden Fred L. , Steinke Robert C. , Talbot Cary A.
Published: February 2015

We have developed a one‐dimensional numerical method to simulate infiltration and redistribution in the presence of a shallow dynamic water table. This method builds upon the Green‐Ampt infiltration with Redistribution (GAR) model and incorporates features from the Talbot‐Ogden (T‐O) infiltration and redistribution method in a discretized moisture content domain. The redistribution scheme is more physically meaningful than the capillary weighted redistribution scheme in the T‐O method. Groundwater dynamics are considered in this new method instead of hydrostatic groundwater front. It is also computationally more efficient than the T‐O method. Motion of water in the vadose zone due to infiltration, redistribution, and interactions with capillary groundwater are described by ordinary differential equations. Numerical solutions to these equations are computationally less expensive than solutions of the highly nonlinear Richards' (1931) partial differential equation. We present results from numerical tests on 11 soil types using multiple rain pulses with different boundary conditions, with and without a shallow water table and compare against the numerical solution of Richards' equation (RE). Results from the new method are in satisfactory agreement with RE solutions in term of ponding time, deponding time, infiltration rate, and cumulative infiltrated depth. The new method, which we call “GARTO” can be used as an alternative to the RE for 1‐D coupled surface and groundwater models in general situations with homogeneous soils with dynamic water table. The GARTO method represents a significant advance in simulating groundwater surface water interactions because it very closely matches the RE solution while being computationally efficient, with guaranteed mass conservation, and no stability limitations that can affect RE solvers in the case of a near‐surface water table.
Ultrafast Self‐Assembly of Graphene Oxide‐Induced Monolithic NiCo–Carbonate Hydroxide Nanowire Architectures with a Superior Volumetric Capacitance for Supercapacitors
Authors: Yang Juan , Yu Chang , Fan Xiaoming , Zhao Changtai , Qiu Jieshan
Published: February 2015

The monolithic electrodes with high volumetric capacitance demonstrate a great potential in practical industrial applications for supercapacitors. Herein, a novel strategy for ultrafast self‐assembly of graphene oxides (GO)‐induced monolithic NiCo–carbonate hydroxide (NiCo–CH) nanowire composite films (G–CH) is reported. The oxygen‐containing functional groups on the GO surface help effectively to induce formation of the monodisperse NiCo–CH nanowires. Such a nanowire‐shaped structure further functions as a scaffold and/or support, leading to 25 s of ultrafast self‐assembly for G–CH composite films and a relatively loose and open channel that contributes to fast electrolyte transport. The as‐obtained monolithic G–CH architectures show an excellent supercapacitor performance as binder‐ and conductive agent‐free electrode, evidenced by a superior volumetric capacitance of 2936 F cm−3 and good electrochemical stability. Combining highly conductive carbon nanotubes (CNTs) into the monolithic composite films can further create well‐interconnected conductive networks within the electrode matrix, thus to improve the reaction kinetics and rate capability. The present strategy that can modulate the growth of the high‐electroactive pseudocapacitive hydroxides and achieve an ultrafast self‐assembly of monolithic composites may pave a promising new way for development of high‐performance supercapacitors and shed a new light on the configuration of carbon‐based electrode materials in energy storage and conversion devices.
Production of Monodisperse Gold Nanobipyramids with Number Percentages Approaching 100% and Evaluation of Their Plasmonic Properties
Authors: Li Qian , Zhuo Xiaolu , Li Shuang , Ruan Qifeng , Xu Qing‐Hua , Wang Jianfang
Published: February 2015

Gold nanobipyramids (NBPs) and nanorods (NRs) are two common types of elongated colloidal plasmonic metal nanocrystals, with their longitudinal plasmon wavelengths synthetically tunable over a wide spectral range. Au NBPs have sharper tips and narrower shape and size distributions than Au NRs. However, the number percentages of Au NBPs have been limited below ≈60%. Herein, a method for producing Au NBPs with number percentages approaching 100% and longitudinal plasmon resonance wavelengths synthetically tuned from ≈700 to ≈1200 nm is reported. This method relies on a stepwise combination of seed‐mediated growth, Ag overgrowth, depletion force‐induced self‐separation, and final chemical etching of Ag. The obtained Au NBPs have the same shapes and sizes as the directly grown ones. Systematic comparisons of the plasmonic properties between the purified Au NBP and high‐yield single‐crystalline Au NR samples show unambiguously that Au NBPs are superior to Au NRs in terms of the plasmon peak width, refractive index sensitivity, figure of merit, two‐photon photoluminescence, and surface‐enhanced Raman scattering.
In Vivo Repeatedly Charging Near‐Infrared‐Emitting Mesoporous SiO2/ZnGa2O4:Cr3+ Persistent Luminescence Nanocomposites
Authors: Li Zhanjun , Zhang Yuanwei , Wu Xiang , Wu Xiaoqiong , Maudgal Rohit , Zhang Hongwu , Han Gang
Published: February 2015

Near‐infrared (NIR) persistent phosphor ZnGa2O4:Cr3+ (ZGC) has unique deep‐tissue rechargeable afterglow properties. However, the current synthesis leads to agglomerated products with irregular morphologies and wide size distributions. Herein, we report on in vivo rechargeable mesoporous SiO2/ZnGa2O4:Cr3+ (mZGC) afterglow NIR‐emitting nanocomposites that are made by a simple, one‐step mesoporous template method. At less than 600 °C, pores in mesoporous silica nanoparticles (MSNs) act as nanoreactors to generate in situ ZnGa2O4:Cr3+ NIR‐persistent phosphors. The as‐synthesized mZGC preserves defined size, morphology, and mesoporous nanostructure of the MSNs. The persistent luminescence of the as‐synthesized mZGC is recharged in a simulated deep‐tissue environment (e.g., ≈8 mm pork slab) in vitro by using red light (620 nm). Moreover, mZGC can be repeatedly activated in vivo for persistent luminescence imaging in a live mouse model by using white LED as a light source. Our concept of utilizing mesoporous silica as nanoreactor to fabricate ZGC PL nanoparticles with controllable morphology and preserved porous nanostructure paves a new way to the development and the wide application of deep tissue rechargeable ZGC in photonics and biophotonics.
Multiporige Zeolithe: Synthese und Anwendungen bei der Katalyse
Authors: Moliner Manuel , Martínez Cristina , Corma Avelino
Published: February 2015

In den letzten Jahren wurde intensiv an der Synthese multiporiger Zeolithe gearbeitet, die Kanäle verschiedener Größen in ihrer Kristallstruktur enthalten. Dieses Thema ist so faszinierend, weil Poren mit verschiedenen Größen die bevorzugte Diffusion von Reaktanten und Produkten durch verschiedene Kanalsysteme begünstigen und damit besondere katalytische Aktivitäten für spezifische chemische Vorgänge ermöglichen können. Hier beschreiben wir die interessantesten Entwicklungen zur rationalen Synthese multiporiger Zeolithe mit kleinen bis extragroßen Poren sowie Verbesserungen, die für relevante chemische Verfahren mit diesen multiporigen Zeolithen als Katalysatoren erhalten werden können.
Tailored Synthesis of a Nonlinear Optical Phosphate with a Short Absorption Edge
Authors: Zhao Sangen , Gong Pifu , Luo Siyang , Bai Lei , Lin Zheshuai , Tang Yuanyuan , Zhou Yuelan , Hong Maochun , Luo Junhua
Published: February 2015

A nonlinear optical phosphate Ba5P6O20 was rationally developed by a tailored synthetic approach based on the use of flexible [P3O10]5− units. The phosphate exhibits a very short absorption edge of λ=167 nm, which is among the shortest known in phase‐matchable phosphates. First‐principles electronic structure analysis elucidated the origin of the changes in the optical properties, and specifically in the absorption edge, of the material. Such a tailored synthetic approach provides a new opportunity to design nonlinear optical materials with short absorption edges.
Monitoring Ligand‐Induced Conformational Changes for the Identification of Estrogen Receptor Agonists and Antagonists
Authors: Mayer‐Wrangowski Svenja C. , Rauh Daniel
Published: February 2015

Nuclear receptors are transcription factors that are important targets for current drug discovery efforts as they play a role in many pathological processes. Their activity can be regulated by small molecules like hormones and drugs that can have agonistic or antagonistic functions. These ligands bind to the receptor and account for diverse conformational changes that are crucial determinants for the receptor activity. Here, we set out to develop FLiN (fluorescent labels in nuclear receptors), a direct binding assay that detects conformational changes in the estrogen receptor. The assay is based on the introduction of a cysteine residue and subsequent specific labeling of the receptor with a thiol‐reactive fluorophore. Changes in the receptor conformation upon ligand binding lead to differences in the microenvironment of the fluorophore and alter its emission spectrum. The FLiN assay distinguishes between different binding modes and is suitable for high‐throughput screening.
A High‐Pressure Polymorph of Phosphorus Oxonitride with the Coesite Structure
Authors: Baumann Dominik , Niklaus Robin , Schnick Wolfgang
Published: February 2015

The chemical and physical properties of phosphorus oxonitride (PON) closely resemble those of silica, to which it is isosteric. A new high‐pressure phase of PON is reported herein. This polymorph, synthesized by using the multianvil technique, crystallizes in the coesite structure. This represents the first occurrence of this very dense network structure outside of SiO2. Phase‐pure coesite PON (coe‐PON) can be synthesized in bulk at pressures above 15 GPa. This compound was thoroughly characterized by means of powder X‐ray diffraction, DFT calculations, and FTIR and MAS NMR spectroscopy, as well as temperature‐dependent diffraction. These results represent a major step towards the exploration of the phase diagram of PON at very high pressures and the possibly synthesis of a stishovite‐type PON containing hexacoordinate phosphorus.