bluuebunny/crossref_metadata_2025 huggingface dataset:
LncRNA FLVCR1-AS1 functions as competing endogenous RNA (ceRNA) to sponge miR-381-3p and aggravate colorectal cancer via upregulation of Wnt signaling pathway
Authors: Beni faeze ahmadi , Abdolvand Mohammad , Salehi Mansoor , Dehghanian Fariba , Kazemi Mohammad
Published: September 2023

Introduction
Colorectal cancer (CRC) is the third most frequent cancer and the second deadliest cancer, worldwide. Long non-coding RNAs (lncRNAs) have been introduced as vital regulators of CRC. lncRNA feline leukemia virus subgroup C receptor 1 antisense RNA 1 (FLVCR1‑AS1) is suggested to play a significant role in the tumorigenesis of several cancers. Wnt signaling pathway is the most deregulated pathway in CRC. The present study aimed to investigate the underlying mechanism of function of FLVCR1-AS1 in CRC through FLVCR1-AS1/miR-381-3p/ CTNNB1, LRP6, FZD3 axis.
Methods
The expression level of FLVCR1-AS1 was compared between CRC tissues and adjacent normal tissues, and additionally between CRC cell lines. Knockdown of FLVCR1-AS1 was performed in HCT116 cells, afterwards, the effects of this knockdown on the expression levels of FLVCR1-AS1, miR-381-3p, and three genes was examined via Real time-PCR. The differences in proliferation were evaluated using MTT assay, and cell death was assessed by flow cytometry.
Results
The results confirmed that FLVCR1-AS1 was upregulated in CRC tissues compared to adjacent normal tissues. RT-qPCR validated that FLVCR1-AS1 has the most level of expression in HT29, HCT116, SW480, and Caco2; respectively. Knockdown of FLVCR1‑AS1 was significantly followed by attenuated viability of HCT116 cells; while resulted in enhanced apoptosis and necrosis.
Conclusion
These findings support the idea that FLVCR1-AS1 may act as an oncogene in CRC and targeting FLVCR1-AS1/miR-381-3p/ CTNNB1, LRP6, FZD3 axis may be introduced as a novel target for CRC therapy and diagnosis in the future.
Dual-energy computed tomography is a superior diagnostic performance for locating parathyroid tumors
Authors: Wang Qian , Yang Wenjing , Wang Jiacheng , Zhao Teng , Feng Dalin , Liu Xing , Shen Hong , Wei Bojun
Published: September 2023

Purpose
To analyze the accuracy of three imaging methods for locating parathyroid tumors preoperatively in patients with primary hyperthyroidism (PHPT).
Methods
We retrospectively analyzed the accuracy of dual-energy computed tomography (DECT), cervical ultrasound (US), and 99m-technetium-labeled methoxyisobutylisonitrile single-photon emission computed tomography/computed tomography (99mTc-MIBI SPECT/CT) for the preoperative localization of abnormal parathyroid glands in 277 patients, who underwent parathyroidectomy from February 2017 to March 2023 in our department. We compared the diagnostic efficiency of the three imaging methods for localization of abnormal parathyroid glands, including those with single parathyroid adenomas or carcinomas, double adenomas (DAs), multiple gland hyperplasia disease (MGHD), and ectopic parathyroid tumors.
Results
A total of 283 pathological parathyroid glands were found in the 277 PHPT patients. The DECT sensitivity of 93.3% was superior to that of US at 92.2% and 99mTc-MIBI SPECT/CT at 75.6%. Ectopic locations were diagnosed by DECT in 12/13 (92.3%) patients by 99mTc-MIBI SPECT/CT in 11/13 (84.6%) patients and by US in 8/13 (61.5%) patients. The diagnostic efficiency was 98.59% for the combination of US and DECT, 97.17% for MIBI and DECT, and 95.76% for US and 99mTc-MIBI SPECT/CT. The diagnostic efficiency of the combination of all three methods for identifying pathological parathyroid glands was 99.29%.
Conclusion
DECT has a higher diagnostic accuracy rate than US and 99mTc-MIBI SPECT/CT for the preoperative localization of abnormal parathyroid glands, and it has advantages for the localization of ectopic parathyroid glands. DECT shows promise as a first-line preoperative localization imaging method for PHPT patients and may lead to significantly improved preoperative localization of pathological parathyroid glands when combined with US and 99mTc-MIBI SPECT/CT.
Genetic Variability of SLCO1B3 and Its Influence on Lipid Levels and Statin Efficacy in Han and Uighur Populations
Authors: Luo Sifu , Jin Menglong , Jureti Subinuer , Ji Hongyu , Liu Ziyang , Maimaitituersun Gulinigaer , Meng Fanhua , Liu Seng , Wei Mengwei , Cao Zichen , Fu Zhenyan
Published: September 2023

Background
Lowering plasma LDL cholesterol levels reduces the risk of atherosclerotic cardiovascular disease (ASCVD). Genetic factors, including single nucleotide polymorphisms (SNPs), affect lipid abnormalities. Statins are the main treatment for lipid disorders, but their effectiveness is influenced by genetics.
Objective
This study examines the impact of SLCO1B3 genetic variability on lipid levels and statin efficacy, particularly on statin sensitivity.
Methods
780 coronary atherosclerotic heart disease patients on oral statins for ≥ 4 weeks were studied. Clinical data and SNP genotyping were collected.
Results
Statin therapy effectively reduces TC, TG, LDL-C, APOA1, and APOB in Han and Uighur populations, with limited impact on HDL-C and Lpa. In the Han population, rs2117032 significantly affects APOB levels, post-statin APOB, Lpa, AST levels, and rate of LDL-C reduction. In Uighurs, rs2117032 significantly affects Tbil levels. In Han individuals, rs3764006 significantly influences TBil, ALT levels, post-statin LDL-C, APOB, Lpa, AST levels, and rates of LDL-C and APOB reduction, correlating with statin sensitivity. In Uighurs, rs3764006 notably affects APOA1 levels. For Han population, rs4149117 significantly impacts LDL-C, TC, TG levels, post-statin TG, ALT, AST levels, and LDL-C reduction. In Uighurs, rs4149117 affects post-statin AST and correlates with statin sensitivity. Additionally, rs2417940 significantly affects Tbil, post-statin TG, and rate of LDL-C reduction in Han, and such effects in Uighurs. In Han population, H3 (C-G-G) haplotype frequencies are higher in statin-sensitive individuals (OR = 1.861, 95% CI: 1.178–2.939, p = 0.007). In Uighurs, H6 (T-A-T) haplotype frequencies are higher in statin-sensitive subjects (OR = 4.906, 95% CI: 1.549–15.541, p = 0.0029).
Conclusion
The genetic polymorphism of SLCO1B3 significantly impacts blood lipid levels, influences liver function to some extent, concurrently exerts a notable influence on the efficiency of statin-induced LDL-C reduction, and is also associated with statin drug sensitivity.
Caregiving Experiences with Health Information Management and Communication in Complex Home Care: Informing Technology Design for Caregivers of Older Adults
Authors: Tennant Ryan , Allana Sana , Mercer Kate , Burns Catherine M.
Published: September 2023

Purpose: The objective of this study was to understand how caregivers manage and communicate health information for older adults who require complex home care, informing the design of new technologies to support patient safety in the home.
Methods: The research involved semi-structured interviews with 15 caregivers, including family and hired caregivers, in Ontario, Canada. An inductive analysis was used to develop themes.
Results: The findings described how participants were Updating the Caregiver Team to share health information in the home. Participants were also Learning to Improve Care & Decision-Making. However, sometimes participants experienced Conflicts within Caregiver Teams using current technologies, which may not fully meet their information management and communication needs.
Conclusion: This research highlights the difficulties of caring for older adults in complex home care situations and the challenges that family and hired caregivers face when managing health information and communication. Currently, paper-based technologies are used, but there is a growing interest in digital tools that can efficiently gather and transform health information to better support decision-making. Collaborative digital systems involving family caregivers as important care team members could improve information sharing and reduce conflicts. However, implementing new technologies in this context can be difficult, and successful adoption may require systems that improve the overall caregiving experience in complex environments. This study recommends integrating caregivers as collaborators and implementing two-way communication in digital systems to enhance caregiver satisfaction. Future research should delve deeper into these complexities and prioritize designing effective tools for this crucial caregiving domain.
The Involvement of the NLRP3 Inflammasome and Pyroptosis in Methamphetamine-Induced Neurotoxicity: Effects on Hippocampal Structure and Memory
Authors: Kurawa Musa Ibrahim , Torkaman-Boutorabi Anahita , Hassanzadeh Gholamreza , Zahmatkesh Maryam , Vousooghi Nasim , Zarrindast Mohammad-Reza , Gholaminejhad Morteza
Published: September 2023

It is known that addictive doses of methamphetamine are neurotoxic; However, it remains unclear whether chronic administration of a lower dose (5 mg/kg) of methamphetamine causes similar neurotoxic effects. This study aimed to assess the effects of chronically administering 5 mg/kg of methamphetamine daily for 1, 2, and 3 weeks on spatial memory, object recognition, passive avoidance memory, hippocampal morphology, and neuroinflammation. Hippocampal morphology was assessed using Nissl stain. The expressions of NLRP3, ASC, and caspase-1 genes were measured as markers of neuroinflammation. Chronic administration of 5 mg/kg of methamphetamine led to significant activation of the inflammasomes (NLRP3, ASC, and caspase-1), resulting in pyramidal cell death in the hippocampus. It also caused impaired spatial memory and passive avoidance learning while leaving recognition memory unaffected. The observed pyramidal cell death is likely due to pyroptosis rather than apoptosis. In conclusion, prolonged administration of 5 mg/kg of methamphetamine was associated with severe inflammasome activation, pyramidal cell death, and mild cognitive decline. Contrary to previous beliefs, even lower doses of methamphetamine taken for an extended period can be neurotoxic.
Language Identity Formation of Second Language Graduate Students in the United States
Authors: Ebrahimi Marzieh
Published: September 2023

Taking social identity theory approach, in this qualitative phenomenological study, researchers investigated how international graduate students from three Asian nations in the United States establish their language identities. The study employs informal interviews with six individuals, from three Asian nations, to inquire about the significance of their individual experiences as they relate to the formation of their linguistic identities. Semi-structured interviews were used to obtain this data since they allowed for an in-depth examination of the participants' experiences. Interviews were analyzed using inductive, thematic data analysis to determine overarching themes. Initial results show that learning a new language presents substantial obstacles for students studying abroad. These include difficulties with pronunciation, communication, and adjustment to culture. Taking language classes and making friends with local students prove to be significant identity-forming experiences. Identity and cultural adaptation are also shown to be influenced by American institutions, such as universities and their professors. The findings of this study can have important significance for institutions which may utilize them to improve the services they offer to international students and create a more welcoming and supportive atmosphere. The study acknowledges its own limitations, including a relatively small sample size and a narrow emphasis on language identity, indicating that more research is needed to investigate the role of language in the formation of international students' identities.
Intraoperative motor-evoked potential with tetanic stimulation changes pre- and post-hemispherotomy
Authors: Sasaki Ryota , Tamura Kentaro , Takatani Tsunenori , Park Young-Soo , Nakagawa Ichiro
Published: September 2023

Careful examination of motor evoked potential (MEP) findings is critical to the safety of intraoperative neuromonitoring during neurosurgery. We reviewed the intraoperative MEP findings in a pediatric patient who had undergone hemispherotomy for refractory epilepsy. The patient was a four-year-and-two-month-old boy with extensive right cerebral hemisphere, drug-resistant epilepsy, left upper and lower extremity paralysis, and cognitive impairment. We examined intraoperative MEP results both before and after hemispherotomy. Post-hemispherotomy, MEPs were successfully elicited through transcranial electrical stimulation (TES) but not via direct cortical stimulation on the right side. Furthermore, TES on the right side, following hemispherotomy, led to a reduction in the MEP amplification effect resulting from tetanic stimulation of the left unilateral median and tibial nerves. Conversely, we observed the effects of MEP amplification during TES on the left side after tetanic stimulation of these nerves. Postoperatively, the patient underwent magnetic resonance imaging and electroencephalogram examinations, confirming the anatomical and electrophysiological completeness of the dissection. Notably, the seizures disappeared, and no apparent complications were observed. Collectively, our findings suggest that TES can still activate deep structures and elicit MEPs, even in cases where the corticospinal connections to the posterior limb of the internal capsule are entirely severed. Thalamocortical interactions may affect the MEP amplification, observed during tetanic stimulation. Injury to the corticospinal tracts of the white matter may be obscured on conventional MEP findings; however, it may be identified by MEP changes in tetanic stimulation.
The combination of Angelica sinensis polysaccharide and cisplatin promotes ferroptosis in cisplatin-resistant ovarian cancer cells by regulating GPX4, thereby reversing their resistance to cisplatin
Authors: Guo Weikang , Wang Wanyue , Lei Fei , Zheng Ruxin , Zhao Xinyao , Gu Yuze , Yang Mengdi , Tong Yunshun , Wang Yaoxian
Published: September 2023

Background
Angelica sinensis (Oliv.) Diels has been used for centuries in Chinese traditional medicine to treat gynecological ailments. Numerous studies indicate that Angelica sinensis polysaccharide (ASP), an extract from Angelica sinensis, can inhibit various forms of cancer. Nevertheless, the therapeutic potential of ASP for treating ovarian cancer remains largely unexplored.
Methods
The study investigated cell proliferation and invasion, as well as the effects of a combination of ASP and DDP after tumor formation in nude mice. Tumor size was monitored, and HE staining was conducted on tissue samples. The identification of key gene GPX4 was performed via RNA-seq and bioinformatic analysis. GPX4 was overexpressed using lentivirus transfection, and its expression was evaluated via RT-qPCR and western blot. Additionally, the ferroptosis of cells was assessed through the measurement of Fe2+, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH).
Results
The results indicated a synergistic effect of ASP combined with DDP, leading to better inhibition of proliferation and invasion of SKOV3/DDP cells. Similarly, ASP combined with DDP demonstrated tumor proliferation inhibition in tumor-forming nude mice, while maintaining good safety. Bioinformatics analysis of 843 differentially expressed genes (DEGs) revealed that the key gene GPX4 played a significant role in the mechanism of action. Furthermore, the expression of GPX4 was inhibited by ASP combined with DDP, which resulted in SKOV3/DDP inhibition of proliferation and invasion. The study also demonstrated that ASP combined with DDP led to increased levels of Fe2+ and MDA, while decreasing levels of GSH and SOD, suggesting the promotion ferroptosis of SKOV3/DDP cells.
Conclusions
The combination of ASP and DDP has the ability to inhibit the proliferation and invasion of SKOV3/DDP cells. Additionally, inhibiting GPX4 expression in SKOV3/DDP cells promotes ferroptosis.
Highl diverse cuticular hydrocarbon profiles but no evidence for aggression towards non-kin in the ambrosia beetle Xyleborinus saxesenii
Authors: MELET A , LEIBOLD V. , SCHMITT T. , BIEDERMANN P.H.
Published: September 2023

Animal societies have nestmate-recognition to protect against social cheaters and parasites. In most social insect societies individuals recognize and exclude any non-nestmate. There, the roles of cuticular hydrocarbons as recognition cues are well documented. Some ambrosia beetles live in cooperatively breeding societies, within nests that are almost completely isolated. Their fungus-farming and inbreeding habits within their nest ensure a nearly complete isolation. However, little is known about whether nestmate recognition is present and adaptive in these ambrosia beetle societies.
Here we studied the ability for nestmate recognition in the cooperatively breeding ambrosia beetle Xyleborus saxesenii, combining behavioural observations and cuticular hydrocarbon analyses. Laboratory nests of X. saxesenii were exposed to foreign adult females, from the same population, another population and another species and survival as well as behaviours of receivers and the donor individual were observed. We expected that increasing genetic distance would cause increasing distance in chemical profiles and increasing levels of behavioural exclusion and possibly mortality. Chemical profiles were different between populations and appeared as variable as in other highly social insects. However, we did not find any evidence for behavioural exclusion of foreign individuals. These results suggest that cuticular hydrocarbon profiles might be used for communication, but that exclusion of non-nestmates is either absent in X. saxesenii or agonistic encounters are so rare or subtle that they could not be detected by our method. In the first case it may be adaptive for inbreeding ambrosia beetle species to accept non-nestmate to increase genetic diversity within nests.
Exsitu assessment of potential free (FC) and immobilized cell (IC) bacteria used as engine oil (UEO) degraders in the degradation of UEO in liquid yeast extract minimal salt medium (YEMSM)
Authors: Elhamrouni Izeddin Abdalla , Ishak Mohd Yusoff , Jaber Saja Mahdey , Kadhum Safaa A , Johari Wan , Halimoon Normala , Awadh Hassan
Published: September 2023

Used engine oil (UEO) contains potentially toxic substances that harmful humans and the ecosystem. Its dangerous pollution requires customized, efficient adsorptive bioremediation. This study investigates the local soil bacteria that can remove petrol-UEO (shell 15–40) by metabolic capability in UEO-YEMSM, previously isolated from UEO-polluted soil in KL, Malaysia. The biosorptive bioremediation capabilities of the free cell (FC) formula for UEO have been studied using pure Ochrobacterium intermedium LMG 3301 and mixed culture consortia of Ochrobacterium intermedium LMG 3301 plus Bacillus paramycoides MCCC1A04098 (BC), with an inoculation size of 3 × 109 CFU/mL in 0.675% (v/v) UEO-YEMSM. To study the UEO adsorption bioremediation of IC systems and compare them to the same FC systems, pure and mixed bacteria cells were immobilized using alginate-attapulgite-calcium carbonate (AAC). On day 24, the IC systems exhibited the removal of nC9 to nC17 with a 30% increase in TPH obtained with O.intermedium and the 18% by O. intermedium + B. paramycoides (BC) in UEO, as compared to the same FC systems. The AAC-IC of O. intermedium and the BC also removed nC9-nC29 to 93% and 98%, demonstrating the adsorptive-biosorptive bioremediation capacity of IC-systems with efficient mass transfers of experimental effectiveness factor (η) values of 1.04, and1.05, close to FC system η = 1. The enhanced degradation and bacterial growth in the AAC-IC systems indicated the high potential of AAC beads to be applied in the insitu bioremediation of UEO-polluted soils/water environments in KL, Malaysia.
An investigation of the breaking strength of timber from different tree species used for school furniture in Walukuba Division, Jinja City, Uganda
Authors: Napulu George , Balimunshe John , balinya lincoln , Nzala Nicholas Walter , Kabagambe Musa
Published: September 2023

The study investigated about the breaking strength of wood in Walukuba Division Jinja City. In this study we aimed to: to determine the breaking strength of different wood types varying length and extension while keeping force and area constant and to determine the breaking strength of different wood types by varying force and length while keeping area and extension constant. In this experiment we studied timber from Eucalyptus, Maesopsis eminii and Markhamia because they are the most common species in the study area. The cantilever method was used where a known mass was attached to the free end of a wood rod and the extension was noted. The experiment was repeated with various masses from 100 to 600grams. We varied the length of wood and kept noting the extension this time round varying the length of the wooden rod while maintaining a load of 100g and noting the extension on the meter rule that was clamped vertically at the extreme end using a retort stand. The results showed the young’s modulus of elasticity of 1267.27,1164.74, and 709.72Nm− 2 for Eucalyptus, Musizi and Makhamia timber respectively. With these results we deduce that timber form Eucalyptus could withstand higher load without breaking as compared to others we recommend that Eucalyptus should be used in generation of furniture for institutions moving forward.
Identification and Validation of Golgi Apparatus-Related Signature for Predicting Prognosis and Immunotherapy Response in Breast Cancer
Authors: Chen Xin , Tang Pengting , Kong Ying , Chen Deqin , Tang Kejun
Published: September 2023

Background: The Golgi apparatus plays a pivotal role in various aspects of cancer. This study aims to investigate the predictive value of Golgi apparatus-related genes (GARGs) in breast cancer prognosis and immunotherapy response evaluation.
Methods: Transcriptional and clinical data from the TCGA-BRCA cohort and GSE96058 cohort were utilized to construct and validate a prognostic model for breast cancer using Cox regression analysis. Differences in immune landscape, somatic mutations, gene expression, drug sensitivity, and immunotherapy response between different risk groups were assessed. A prognostic nomogram for breast cancer was further developed and evaluated. qPCR and single-cell sequencing analysis were performed to validate the expression of GARGs.
Results: A total of 394 GARGs significantly associated with breast cancer prognosis were identified, leading to the construction of a prognostic risk feature comprising 10 GARGs. This feature effectively stratified breast cancer patients into high-risk and low-risk groups, with the high-risk group exhibiting significantly worse prognosis. Meanwhile, significant differences in cliniopathological features, immune infiltration, drug sensitivity, and immunotherapy response were observed between the high- and low-risk groups. The constructed nomogram incorporating these factors showed superior performance in prognostic assessment for breast cancer patients. Ultimately, the utilization of qPCR and single-cell sequencing techniques substantiated the disparate expression patterns of these prognostic genes in breast cancer.
Conclusions: Our findings demonstrate that a prognostic risk feature derived from GARGs holds promising application potential for predicting prognosis and evaluating immunotherapy response in breast cancer patients.
Biodegradable Materials of Oat Hulls, Starch, and Polybutylene Succinate Produced by Thermoplastic Injection
Authors: Silva Samuel Camilo , Carvalho Fabíola Azanha , Yamashita Fabio
Published: September 2023

The utilization of biopolymers blended with agro-industrial residues or by-products holds promise due to the potential for cost reduction and improvement of material properties. In this study, our aim was to develop biodegradable polymeric blends using PBS, starch, and oat hulls via thermoplastic injection. Seven different formulations were analyzed [F0, F20, F40, F60, F80, F100, and PBS], and various analyses were performed. The mechanical properties revealed that pure PBS and F0 presented the highest tensile strength and elongation at break respectively. Pure PBS and F100 demonstrated the lowest density. SEM of materials containing oat hulls displayed cylindrical-shaped fibers, with significant agglomeration and alignment likely due to extrusion orientation. The results demonstrate the successful production of a novel material using thermoplastic starch, PBS, and oat hulls through thermoplastic injection. These materials exhibited good processability. This presents a promising avenue for the development of cheaper and biodegradable materials with good processability to be produced in large scale, as they are environment friendly.
Local Behavior of Certain n-Dimensional Diffeomorphisms
Authors: Siller Tiago E.
Published: September 2023

The main purpouse of this paper is to prove that a \(C^1\)-diffeomorphism of the form \(\varphi(x) = x + (x_n\varphi_1(x), \ldots, x_n\varphi_{n-1}(x), x_n^2\varphi_n(x))\) is locally topologically equivalent to its linear part under a generic condition.
MSC Classification: 37C15 , 37C05 , 58K40
RDSL: an Efficient Retrieval Algorithm for Group Editing CRDT
Authors: Ou Yuran , Zhou Jian
Published: September 2023

Conflict-Free Replicated Data Types (CRDT) is a popular approach for underpinning group editing in many applications, providing features such as peer-to-peer and offline editing. While conventional approaches such as Operation Transformation (OT) can be challenging to implement those features, CRDT still faces a critical issue - inefficient data retrieval due to storing data in a data structure with linear complexity. To address the issue, we propose a novel retrieval algorithm called Relative Distance Skip List (RDSL). This algorithm utilizes a probabilistic balancing identifier structure, which is based on relative distances between original CRDT nodes. The RDSL algorithm is both time- and space-efficient, providing a stable and efficient solution for data retrieval in CRDT.
Evolution of the urinary microbiota in spinal cord injury patients with decubitus ulcer: a snapshot study
Authors: Morsli Madjid , Salipante Florian , Gelis Anthony , Magnan Chloé , Guigon Ghislaine , Lavigne Jean-Philippe , Sotto Albert , Dunyach-Remy Catherine
Published: September 2023

Background Current microbiome investigations of patients with pressure ulcers (PU) are mainly based on wound swabs and/or biopsy sequencing, leaving the colonization scenario unclear. Urinary microbiota has been never studied.Methods As part of the prospective ESCAFLOR study, we studied urinary microbiota of spinal cord injury (SCI) patients with PU without any urinary tract infection at the inclusion, collected at two times (at admission (D0) and after 28 days (D28)) during the patient's care (Trial Registration Number; South Mediterranean III; N°2014.04.01bis), investigated by 16S rDNA metagenomics next generation sequencing. Subgroup analyses were carried out between patients with wounds showing improved evolution versus stagnated/worsened wounds at D28. Analysis was done using EPISEQ® 16S and R software.Results Among the 12 studied patients, the urinary microbiota of patients with improved wound evolution at D28 (n = 6) presented a significant decrease of microbial diversity. This modification was associated with the presence of Proteobacteria phylum and an increase of Escherichia-Shigella (p = 0.005), as well as the presence of probiotic anaerobic bacteria Lactobacillus and Bifidobacterium. In contrast, Proteus abundance was significantly increased in urine of patients with stagnated/worsened wound evolution (n = 6) (p = 0.003).Conclusions This study proposes urinary microbiota as a complementary factor indirectly associated with the wound evolution and patient cure. It opens new perspectives for further investigations based on multiple body microbiome comparison to describe the complete scenario of the transmission dynamics of wound-colonizing microorganisms.
Genetic Variability Studies Based on Morphological and Physicochemical Characteristics Among Different Longan (Dimocarpus longan Lour.) Genotypes Under North Bihar Conditions
Authors: Kumar Ajay , Gupta A. K. , Kanth Neeharika , Yadav Sudheer Kumar
Published: September 2023

The present experiment was carried out in a randomized complete block design (RCBD) with three replications to study morphological variability, genetic variability, heritability, and genetic advance for different traits of twenty genotypes. The results indicated that the genotypes were diversified for all the quantitative and qualitative traits except a few qualitative traits. The phenotypic coefficient of variation (PCV) showed higher than the genotypic coefficient of variation (GCV) which indicates the meager effect of environment on the phenotypic expression of traits. The high heritability coupled with high genetic advance as percent of mean were recorded for the traits viz., plant height, trunk girth, crown diameter, tree volume, rachis and petiole length, duration of panicle imitation, number of days from panicle initiation to flowering, length and width of inflorescence, number of fruits per bunch, fruit, aril and seed weight, aril thickness, titratable acidity and TSS acid ratio. Therefore, these traits can be used as selection indices for indirect selection for the crop improvement programmes. On the basis of mean performance found best performing genotypes LGC-6 for yield attributing and fruit quality traits.
Enhanced catalysis of mechano-chemically synthesized KMgF3 catalysts for the Knoevenagel condensation
Authors: Iida Hajime , Sugiyama Shouya , Horie Tatsuya
Published: September 2023

Catalysis of KMgF3 catalysts for Knoevenagel condensation were enhanced by synthesizing mechano-chemically. The BET surface area and number of strongly basic site of KMgF3 were increased by applying greater mechanical energy (higher rotation rate) in a mechano-chemical process. Their increases were caused by stronger mechano-chemical effects such as the micronization of the particles and the creation of lattice defect on the surface, resulted in resulted in the enhancement of catalytic activity of KMgF3 for Knoevenagel condensation. The XPS analysis revealed that the element composition on the surface of KMgF3 was similar with K2MgF4 and indicated the possibility which the true active component for this reaction was K2MgF4 rather than KMgF3. The kinetic measurements revealed that the Knoevenagel condensation catalyzed by KMgF3 was regard as a first order reaction and apparent activation energy was estimated at 55.8 kJmol-1. A soluble component capable of acting as a catalyst was not present in the solution and the KMgF3 acted as a true solid catalyst.
Nutrient stoichiometry mediates the patchy coexistence of two subalpine grassland types
Authors: Hernández Juan Luis Mora , Armas-Herrera Cecilia , Badía-Villas David , Gómez Daniel
Published: September 2023

Background and Aims
High mountain areas often show high soil heterogeneity that allows for the close coexistence of plant species and communities with contrasting resource requirements. This study investigates the nutritional factors driving the mosaic distribution of Nardus stricta L. grasslands and chalk grasslands dominated by forbs in the subalpine southern Pyrenees (Spain).
Methods
The concentrations of C, N, P, S, K, Ca and fiber fractions were analyzed in herbage in relation to soil nutrient availability, soil β–glucosidase, urease, phosphatase and arylsulfatase activity and plant species and functional type composition.
Results
The chalk grassland showed higher N:P ratios in herbage and higher enzyme demand for P relative to N in the soil, which indicate a greater limitation of P versus N compared to the Nardus grassland. This limitation was related to the higher soil and plant Ca levels in the chalk grassland, where the calcareous bedrock lies close to the soil surface. In the Nardus grasslands, the alleviation of P limitation translated into higher productivity and replacement of forbs with taller graminoids rich in structural carbohydrates, which was accompanied by greater β–D–glucosidase activity. The plant N:K and P:K ratios indicated potential K deficiency in both grasslands, which resulted from a decreased uptake of K due to competition from Ca, as indicated by the correlation between plant K and the soil K+:Ca2+ ratio.
Conclusions
Our results highlight the effect of the heterogeneity of soil nutrient constraints, as mediated by their stoichiometry and controlled by local topography, on the biodiversity of high mountain ecosystems.
Fabrication of 3D aligned porous nanocellulose-based aerogel for rapid enrichment of the histidine-containing polypeptides
Authors: Ou Jiahui , Zhou Shuaipeng , Huang Chuanqing Q. , Tang Xiaoyue , Li Lu , Tian Yunong , Ye Yong
Published: September 2023

The high-throughput immobilized metal affinity chromatography (IMAC) material is urgently required to improve the efficiency of separation and purification of tagged-peptides in the related biomedical applications. Herein, the aminothiourea-modified nanocellulose (S-TOCN)-polyvinyl alcohol (PVA) composite aerogel with aligned pores was fabricated by directional freezing casting technique and then used to immobilize metal ion for the selective separation and purification of histidine-containing peptides from protein hydrolysates. The obtained S-TOCN-PVA materials with an oriented porous structure under the optimal conditions exhibited excellent mechanical properties, good adsorption performance and reusability. The adsorption capacity of S-TOCN-PVA aerogel for metal ions were as follows: Cu2+>Ni2+>Zn2+>Co2+, in which the higher metal ion chelating amount of S-TOCN-PVA composite aerogel for Cu2+ and Ni2+ reached 87.21 mg/g at pH=7 and 83.66 mg/g at pH=6 in the 2 h incubation time, respectively. The enrichment effects of Cu2+/S-TOCN-PVA and Ni2+/S-TOCN-PVA aerogels with selectivity of His-containing peptides from apricot kernel protein hydrolysates were up to 3.99 folds and 4.81 folds for one cycle, respectively. Moreover, the Ni2+/S-TOCN-PVA aerogels retained 87.5% of their initial adsorption capacity (848 mg/g) after 5 cycles, and the concentration was raised 39.05 folds. Molecular docking simulation showed that the oxygen of carboxyl group (-C-O and -C=O) and hydrogen of imidazole group at His molecule was primarily bound to the hydrogen of amino groups and oxygen of carboxyl group (C-O-C) on the structure of M2+/S-TOCN-PVA aerogels. The fabricated metal ion immobilized 3D aligned porous nanocellulose-based aerogels can be used as IMAC materials for rapid separation and purification of histidine-containing polypeptides from complicated biological samples.
Thermotherapy combined with alternative products in the management of melon rot caused by Fusarium falciforme
Authors: de medeiros Ana Santana , Alves Tatianne Raianne Costa , Silva Jarlan Lucas dos Santos , de Moura Ana Paula , de Lima Jailma Suerda Silva , de Souza José Janderson Ferreira , de França Mateus Araújo Vasconcelos , Fernandes Juliano da Costa , Evangelista Luiz Fernando Bezerra , Ambrósio Márcia Michelle de Queiroz
Published: September 2023

In the search for efficient and sustainable methods for the management of Fusarium rot in melon, the present study aimed to evaluate the efficiency of the thermotherapy, either alone or in combination with alternative products to Magnate®, in melons inoculated with two isolates of Fusarium falciforme. The effect of the treatments on fruit quality was evaluated using physicochemical parameters, disease severity, and enzymatic analyses. The in vitro experiments showed that the lowest concentration (0.0625% v/v) of cinnamon essential oil was the most effective in controlling the mycelial growth of both isolates, demonstrating comparable effects to those observed with Copper Crop® and Magnate®. In the in vivo experiments, the treatment of fruits with thermotherapy combined with Cooper Crop® greatly reduced melon rot, decreased the damage caused by the two isolates of F. falciforme, and was more effective than Magnate®. This treatment produced a lower level of hydrogen peroxide in the inoculated fruits, thereby maintaining cell membranes. It contains antifungal compounds inherent to its composition and exerts an influence on the enzymes peroxidase and polyphenoxidase, thereby inducing the defense mechanism of the fruit. Therefore, thermotherapy combined with Cooper Crop® can be used in the management of melon rot as an alternative to Magnate®.
A Novel Aging-Related lncRNAs Signature-Based Model for Prognosis Prediction in Pancreatic Cancer
Authors: 李 Xinxiong , wang Meiping , Zhang Zaizhong , Feng Tianshun , Wu Junhong , Hong Weixuan , Fang Junwei , Wang Lie , Zhao Hu , Xiao Chunhong
Published: September 2023

Objective
To identify aging-related long non-coding RNAs (ARLs) with prognostic significance and construct a prognostic model for pancreatic cancer (PC) patients.
Methods
Transcriptome information from PC samples and normal samples was obtained from the Cancer Genome Atlas Database (TCGA) and the Genotypic Tissue Expression Database (GTEx). Aging-related genes (ARGs) were obtained from the Human Aging Genome Resources (HAGR) and GeneCards. Correlation analysis was performed to screen out ARLs. Univariate regression, lasso regression, and multivariate regression were used to identify the target ARLs and construct the prognostic model for aging-related PC.
Results
A total of 1109 ARLs were identified, and 9 target ARLs were obtained to construct the risk score prognostic model. These target ARLs include AC245041.2, AC244153.1, AC091057.1, MIR3142HG, AL137779.2, AC145207.5, TDRKH-AS1, AC068620.2, and AC127024.6. The model showed an area under the curve (AUC) of 0.798 on the receiver operating curve (ROC) curve, indicating its effectiveness in predicting prognosis. Kaplan-Meier analysis demonstrated a significant difference in overall survival (OS) between the two groups based on the median risk (P＜0.001). To further assess prognosis, the risk score was combined with clinicopathological features to construct a nomogram for PC. Additionally, gene enrichment analysis (GSEA) and immunological correlation analysis revealed differences in gene enrichment level, immune infiltration, and the expression of immune checkpoint genes between the two groups.
Conclusion
The constructed prognostic model based on nine ARLs provides valuable insights for the prognosis management of PC patients and the development of promising biomarkers in the diagnosis and treatment of PC.
DCSE-PCN: A Coarse-to-Fine Point Completion Network with Details Compensation and Structure Enhancement
Authors: Miao Yongwei , Jing Chengyu , Gao Weihao , Zhang Xudong
Published: September 2023

To address the limitations on inadequate completion of shape details for traditional methods, a novel coarse-to-fine point completion network (DCSE-PCN) is introduced in this paper through the modules of local details compensation and shape structure enhancement for effective geometric learning. The coarse completion stage of our network consists of two branches — a shape structure recovery branch and a local details compensation branch, which can recover the overall shape of the underlying model and the shape details of incomplete point cloud through feature learning and hierarchical feature fusion. The fine completion stage of our network employs the structure enhancement module to reinforce the correlated shape structures of the coarse repaired shape (such as regular arrangement or symmetry), thus obtaining the completed geometric shape with finer-grained details. Extensive experimental results on the ShapeNet dataset and ModelNet dataset validate the effectiveness of our DCSE-PCN, which can recover the shape details of the underlying point cloud while maintaining its overall shape. Compared to the existing methods, our presented coarse-to-fine completion network is shown its competitive performance on both CD and EMD distance error and is also robust to different degrees of data incompleteness and model noise.
Efficient Use of Auxiliary Attribute in Estimating the Finite Population Mean in Sample Surveys
Authors: Singh Housila P. , Tailor Rajesh , Malviya Priyanka
Published: September 2023

This paper addresses the problem of estimating the finite population mean of the study variable y in the presence of auxiliary attributes. An improved class of estimators for population mean has been defined along with properties under large sample approximation. An empirical study was carried out to observe the performance of the proposed and the existing estimators. It turns out that the suggested estimators are substantially more precise than the recent estimators envisaged by Koyuncu (2012) and Haq and Shabbir (2014, 2018).
Set-valued minimax programming problems under higher-order σ-arcwisely connectivity
Authors: DAS KOUSHIK
Published: September 2023

In this work, a set-valued minimax programming problem (MP) is taken into account. In the broad sense of higher-order arcwisely connected set-valued maps, we introduce the concept of higher-order σ-arcwisely connectedness of set-valued maps. Under higher-order contingent epidifferentiation and higher-order σ-arcwisely connectivity suppositions, the higher-order sufficient criteria for Karush-Kuhn-Tucker (KKT) optimality are constructed for the problem (MP). Additionally, we develop the higher-order Mond-Weir, Wolfe, and mixed kinds of duality and demonstrate the higher-order strong, weak, and converse theorems of duality among our main problem (MP) and the associated duals under higher-order σ-arcwisely connectivity supposition.
Mathematics Subject Classification (2000) 26B25 · 49N15
Estimation of the Exponential Distribution Parameter and Reliability Based on the Kernel and Bayes Approaches
Authors: Maswadah M.
Published: September 2023

We suggest herein a procedure for estimating the distribution parameters and reliability based on the non-parametric kernel density function. This technique has been utilized as an alternative and reliable approach for estimation in life-testing models directly from the data without any prior assumptions about the underlying distribution parameters. The efficiency of this technique has been studied compared to the Bayesian approach for estimating the parameter and reliability of the exponential distribution via Monte Carlo simulations. The simulation results indicated the robustness of the proposed method over the Bayesian approach based on the informative and informative conjugate priors. Finally, a numerical example is given to illustrate the densities and the inferential methods developed in this paper.
Water Quality Assessment of Rispana River at Dehradun (Uttarakhand) India with Reference to WQI and Microbial Contamination.
Authors: Singh Jabrinder , Pant Manish , Lakhera Aman , Singhal Naveen
Published: September 2023

In Dehradun capital city of Uttarakhand state (India) a major water resource Rispana river is highly contaminated due to anthropogenic activities and its water quality and quantity reduced to a critical level in recent past. The work was carried out on spatial basis in 4 different locations in pre-monsoon season during 2022. Sample collection and analysis was done as per APHA in laboratory for various parameters to calculate the water quality index-WQI by adopting the weighted arithmetic index approach. In this study it was observed that WQI varies from47.8 – 299.05 with an average of 146.14 (indicating a very poor quality & unfit for domestic purpose). Coliform levels are also very high (.2419.6 MPN) at majority of sites. River water harvesting at upstream region by municipal authorities led to reduced streamflow and discharge of waste from domestic and run-off sources contaminates the Rispana. Regulatory regimes must be executed for controlling unsustainable development approaches & water pollution in river catchments in Dehradun so that water quality and quantity of river Rispana could be enriched. To rejuvenate the water resources for sustainable utilization is a main mandate for development as per SDG 6.
Apparent Non-Suppression of Chaos in Spatiotemporal Chaotic Systems under Spatial and Temporal Variation of Parameters
Authors: Teli Bishnu Gupta , Kolake Balamurali Krishna Mayya
Published: September 2023

Coupled Map Lattice systems have been extensively studied in the past. The behavior of such systems under various mechanisms for the suppression of chaos has been investigated. The suppression of chaos is investigated here for both spatial as well as temporal variations of parameters. For this, the well-known Logistic map is used as the dynamical system. The method of homogeneous pinning is used as the chaos suppression mechanism. It is found that with pinning conditions, the STC when (i) control parameter r having only spatial variation together with coupling parameter ϵ having either only temporal temporal variation or spatial as well as temporal variation at the same time, (ii) r having temporal variation with every possible variation in ϵ and (iii) r having both spatial as well as temporal variation at the same time with every possible variation in ϵ, appears to have narrow range of values with non-suppression of chaos. However, upon close examination, it is found that the system is not chaotic at all.
Conditional Inference on Pareto Distribution Based on Generalized Order Statistics
Authors: Maswadah M.
Published: September 2023

Conditional and unconditional procedures are used for constructing confidence intervals for Pareto distribution parameters based on generalized order statistics via Monte Carlo simulations. The conditional confidence intervals for the parameters are derived for different sample sizes to allow an assessment of the amount of information lost when one bases confidence intervals on the unconditional distribution. Finally, a numerical example is given to illustrate the inferential methods developed in this paper.
Rotational alignment after intramedullary nailing in trochanteric fractures: Lateral decubitus versus supine position with fracture table. Does operative position matter?
Authors: Yurteri Ahmet , Mercan Numan , Kılıç Mehmet , Temiz Ahmet , Doğar Fatih , Topak Duran , Yıldırım Ahmet
Published: September 2023

Background: Fixation of trochanteric fractures with intramedullary nail in non-physiological position can cause poor functional outcomes. The aim of this study, evaluate the effect of intra-operative patient position on rotational alignment in intramedullary nail fixation of trochanteric fractures.
Methods: Femoral rotational alignment of 84 trochanteric fracture patients who underwent intramedullary nailing was measured by comuted tomography (CT) images. Patients were divided into two groups: Supine position on fracture table (Group-1, n:42) and lateral decubitus (LD) position (Group-2, n:42). Femoral malrotation angles were measured and divided into three subgroups as insignificant, significant and excessive. Number of intra-operative fluoroscopy imaging, preparation time, surgery time and anesthesia time in both groups compared.
Results: Malrotation degrees of patients in Group-1 ranged from 17° external rotation (ER) to 57° internal rotation (IR), with a mean of 10° IR. Of the patients in Group-1, 27 were in insignificant, 5 were in significant, and 10 were in excessive subgroup. Malrotation degrees of patients in Group-2 ranged from 33° ER to 47° IR, with a mean of 11° IR. Of patients in Group-2, 21 were in insignificant, 12 were in significant, and 9 were in excessive subgroup. There was no statistically significant relationship between patient position and malrotation angle. Number of intra-operative fluoroscopy imaging, preparation time and anesthesia time were statistically lower in Group-2. There was no statistically significant difference between Group-1 and Group-2 in terms of surgery time.
Conclusion: Intramedullary nailing in LD position is a reliable and practical surgical method in treatment of femoral trochanteric fractures, since there is no need for use of a fracture table, surgeon is exposed to less radiation, no risk of complications related to traction of fracture table, and shorter operation time.