bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of Host-Associated Microbiota and Isolation of Antagonistic Bacteria from Greater Amberjack (Seriola dumerili, Risso, 1810) Larvae
Authors: Paralika Vasiliki , Kokou Fotini , Karapanagiotis Stelios , Makridis Pavlos
Published: July 2023

Greater amberjack (Seriola dumerili) is a new species in marine aquaculture with high mortalities at the larval stages. The microbiota of amberjack larvae was analyzed using 16S rDNA sequencing in two groups, one added copepod nauplii (Acartia tonsa) in the diet, and one without copepods (control). In addition, antagonistic bacteria were isolated from amberjack larvae and live food cultures. Proteobacteria was the most abundant phylum followed by Bacteroidota in amberjack larvae. The composition and diversity of the microbiota were influenced by age, but not by diet. Microbial community richness and diversity significantly increased over time. Rhodobacteraceae was the most dominant family followed by Vibrionaceae, which showed the highest relative abundance in larvae from the control group 31 days after hatching. Alcaligenes and Thalassobius genera exhibited a significantly higher relative abundance in the copepod group. Sixty-two antagonistic bacterial strains were isolated and screened for their ability to inhibit four fish pathogens (Aeromonas veronii, Vibrio harveyi, V. anguillarum, V. alginolyticus) using a double-layer test. Phaeobacter gallaeciensis, Phaeobacter sp., Ruegeria sp., and Rhodobacter sp. isolated from larvae and Artemia sp. inhibited the fish pathogens. These antagonistic bacteria could be used as host-derived probiotics to improve the growth and survival of the greater amberjack larvae.
PGPB and/or AM Fungi Consortia Affect Tomato Native Rhizosphere Microbiota
Authors: Nasuelli Martina , Novello Giorgia , Gamalero Elisa , Massa Nadia , Gorrasi Susanna , Sudiro Cristina , Hochart Marie , Altissimo Adriano , Vuolo Francesco , Bona Elisa
Published: July 2023

Tomatoes are one of the most important crops worldwide and also play a central role in the human diet. Microbial consortia are microorganism associations, often employed as bioinoculants, that can interact with the native rhizosphere microbiota. The aim of this study was to evaluate the impact of a bacterial-based biostimulant (Pseudomonas fluorescens and Bacillus amyloliquefaciens) (PSBA) in combination, or not, with a commercial inoculum Micomix (Rhizoglomus irregulare, Funnelliformis mosseae, Funnelliformis caledonium, Bacillus licheniformis, Bacillus mucilaginosus) (MYC) on the native rhizosphere communities and on tomato production. The trial was carried out using Solanum lycopersicum in an open field as follows: control full NPK (CFD), control reduced NPK (CRD), MYC, PSBA, PSBA + MYC. Bacterial population in the different samples were characterized using a next generation sequencing approach. The bioinocula effect on the native rhizosphere microbiota resulted in significant variation both in alpha and beta diversity and in a specific signature associated with the presence of biostimulants, especially in the presence of co-inoculation (PSBA + MYC). In particular, the high initial biodiversity shifts in the community composition occurred and consisted in the increase in the abundance of genera correlated to the soil acidification and in an enhanced density of nitrogen-fixing microbes. The results also highlighted the well-known rhizosphere effect.
Inclusion of Quillaja Saponin Clarity Q Manages Growth Performance, Immune Response, and Nutrient Transport of Broilers during Subclinical Necrotic Enteritis
Authors: Blue Candice E. C. , Emami Nima K. , White Mallory B. , Cantley Staci , Dalloul Rami A.
Published: July 2023

Necrotic enteritis (NE) is an intestinal disease that results in poor performance, inefficient nutrient absorption, and has a devastating economic impact on poultry production. This study evaluated the effects of a saponin-based product (Clarity Q, CQ) during an NE challenge. A total of 1200 male chicks were randomly assigned to four dietary treatments (10 pens/treatment; 30 birds/pen): treatment 1 (NC), a non-medicated corn–soybean basal diet; treatment 2 (PC), NC + 50 g/metric ton (MT) of bacitracin methylene disalicylate (BMD); and treatments 3 (CQ15) and 4 (CQ30), NC + 15 and 30 g/MT, respectively. On the day (d) of placement, birds were challenged by a coccidia vaccine to induce NE. On d 8, 14, 28, and 42, performance parameters were measured. On d 8, three birds/pen were necropsied for NE lesions. On d 8 and d 14, jejunum samples from one bird/pen were collected for mRNA abundance of tight junction proteins and nutrient transporter genes. Data were analyzed in JMP (JMP Pro, 16), and significance (p ≤ 0.05) between treatments was identified by Fisher’s least significant difference (LSD) test. Compared to PC and NC, CQ15 had higher average daily gain (ADG), while CQ30 had lower average daily feed intake (ADFI) and feed conversion ratio (FCR). NE lesions in the duodenum were lower in CQ15 compared to all other treatments. On d 8, mRNA abundance of CLDN1, CLDN5, AMPK, PepT2, GLUT2, and EAAT3 were significantly greater in CQ30 (p < 0.05) compared to both PC and NC. On d 14, mRNA abundance of ZO2 and PepT2 was significantly lower in PC when compared to all treatments, while that of ANXA1, JAM3, and GLUT5 was comparable to CQ15. In summary, adding Clarity Q to broiler diets has the potential to alleviate adverse effects caused by this enteric disease by improving performance, reducing intestinal lesions, and positively modulating the mRNA abundance of various tight junction proteins and key nutrient transporters during peak NE infection.
Nutritional Supplement Use in a UK High-Performance Swimming Club
Authors: Newbury Josh W. , Sparks S. Andy , Cole Matthew , Kelly Adam L. , Gough Lewis A.
Published: July 2023

The aim of this study was to observe the nutritional supplement practices of highly trained swimmers on a national talent pathway, since it is often reported that swimmers engage in widespread supplement use at the elite level. Thus, this study employed a validated supplement intake questionnaire with forty-four swimmers from a high-performance swimming club, which had three distinct talent stages: development (aged 11–14 years, n = 20), age-group (aged 13–17 years, n = 13), and national level (aged ≥ 16 years, n = 11). Ninety-eight percent of the interviewed swimmers reported using at least one supplement, with performance (34%) and recovery (19%) cited as the primary reasons. National swimmers used more total supplements (8.1 ± 3.4 supplements) compared to age-group (4.8 ± 2.0 supplements, p = 0.003, g = 1.17) and development (3.9 ± 1.7 supplements, p < 0.001, g = 1.69) swimmers, mostly because of a greater intake of ergogenic aids (2.4 ± 1.4 supplements vs. age-group: 0.5 ± 0.5 supplements, p < 0.001, g = 1.12; vs. development: 0.1 ± 0.2 supplements, p < 0.001, g = 1.81). Parents/guardians were the primary supplement informants of development swimmers (74%, p < 0.001, V = 0.50), whereas performance nutritionists informed ~50% of supplements used by age-group and national swimmers (p < 0.001, V = 0.51). Based on these results, supplement education and greater focus on basic sport nutrition practices may be required for parents/guardians at the development level. Moreover, further research is needed to support the high number of ergogenic aids used by national swimmers, with the efficacy of these supplements currently equivocal in the applied setting.
Higher Dietary Acid Load Might Be a Potent Derivative Factor for Multiple Sclerosis: The Results from a Case–Control Study
Authors: Saeedirad Zahra , Ariyanfar Shadi , Noormohammadi Morvarid , Ghorbani Zeinab , Naser Moghadasi Abdorreza , Shahemi Sahar , Ghanaatgar Milad , Rezaeimanesh Nasim , Hekmatdoost Azita , Ghaemi Amir , Razeghi Jahromi Soodeh
Published: July 2023

This study aimed to investigate the association between dietary acid load (DAL) and multiple sclerosis (MS), through the potential renal acid load (PRAL) and net endogenous acid production (NEAP) scores. In a hospital-based case–control study of 109 patients with MS and 130 healthy individuals, a validated 168-item semi-quantitative food frequency questionnaire and a logistic regression model were used to evaluate the association between the DAL and MS. After adjusting for age (years), gender (male/female), body mass index (Kg/m2), and total calories (Kcal), the MS odds were 92% lower for those in the highest tertile of total plant-based protein (OR: 0.08, 95%CI: 0.03, 0.23; p-value < 0.001) and about four times higher for those in the highest tertile of the PRAL (OR: 4.16, 95%CI: 1.94, 8.91; p-value < 0.001) and NEAP scores (OR: 3.57, 95%CI: 1.69, 7.53; p-value < 0.001), compared to those in the lowest tertile. After further adjusting for sodium, saturated fatty acid, and fiber intake, the results remained significant for total plant-based protein intake (OR: 0.07, 95%CI: 0.01, 0.38; p-value = 0.002). In conclusion, a higher NEAP or PRAL score may be associated with increased odds of MS, while a higher intake of plant-based protein instead of animal-based protein may be protective.
A Global Overview of Dietary Supplements: Regulation, Market Trends, Usage during the COVID-19 Pandemic, and Health Effects
Authors: Djaoudene Ouarda , Romano Anabela , Bradai Yasmine Djedjiga , Zebiri Feriel , Ouchene Amina , Yousfi Yasmine , Amrane-Abider Meriem , Sahraoui-Remini Yasmine , Madani Khodir
Published: July 2023

Over the last 20 years, the use of dietary supplements (DS) has continued to grow in many countries. Due to the public health crisis brought on by the COVID-19 pandemic and amidst fears regarding COVID-19 vaccines and their low supply in many regions of the world, there has been a marked interest in the use of DS as alternative means of protecting against and treating this emerging disease, as well as boosting the immune system and minimizing the risk of inflammation. Despite a lack of evidence to suggest their efficacy, a surge in the sales of DS has been reported in many parts of the world. Questions have also been raised about the health effects associated with DS due to their increased use during the health crisis. Numerous scientific studies have demonstrated their beneficial properties as well as some adverse and even toxic effects. In addition, given the current global interest in this issue, a review is needed to establish the status of dietary supplements before and during the health crisis. The aim of this review is to summarize the current evidence on the impact of dietary supplements on the incidence of the COVID-19 pandemic, as well as their regulation and associated market trends. First, we provide an overview of DS, including a comprehensive review of the legislative and regulatory aspects of DS in the USA, China, the EU, and Algeria. Second, we describe the prevalence of the most commonly consumed DS and their efficacy as a prophylactic modality in the era of COVID-19. Additionally, we examine the structure and size of the DS market in the countries that predominantly produce and import them, its global market trend, and the impact of the COVID-19 pandemic on market growth. Finally, in this review, we also discuss the profile of DS users.
Longer-Term Weight Loss Outcomes Are Not Primarily Driven by Diet Following Roux-en-Y Gastric Bypass and Sleeve Gastrectomy
Authors: Sorgen Alicia A. , Fodor Anthony A. , Steffen Kristine J. , Carroll Ian M. , Bond Dale S. , Crosby Ross , Heinberg Leslie J.
Published: July 2023

Metabolic and bariatric surgery (MBS) is the most effective long-term treatment for Class III obesity. Reduced dietary intake is considered a behavioral driver of post-surgical weight loss, but limited data have examined this association. Therefore, this study examined prospective, longitudinal relationships between dietary intake and weight loss over 24 months following Roux-en-Y Gastric Bypass and Sleeve Gastrectomy. Relationships between weight loss and dietary intake were examined using a validated 24-h dietary recall method. Associations between total energy/macronutrient intake and weight loss outcomes were assessed at 12-, 18-, and 24-months following MBS, defining patients as “responders” and “suboptimal responders”. Consistent with previous literature, 12-month responders and suboptimal responders showed significant associations between weight loss and energy (p = 0.018), protein (p = 0.002), and total fat intake (p = 0.005). However, this study also revealed that many of these associations are no longer significant 24 months post-MBS (p > 0.05), despite consistent weight loss trends. This study suggests a short-term signal between these dietary factors and weight loss outcomes 12 months post-MBS; however, this signal does not persist beyond 12 months. These results are essential for interpreting and designing clinical studies measuring long-term post-surgical weight loss outcomes.
Androgen Receptor and Cardiovascular Disease: A Potential Risk for the Abuse of Supplements Containing Selective Androgen Receptor Modulators
Authors: Hall Ellis , Vrolijk Misha F.
Published: July 2023

The androgen receptor (AR) is a member of the family of ligand-activated transcription factors. Selective androgen receptor modulators (SARMs) exert their biological function through complex interactions with the AR. It has been speculated that overexertion of AR signaling cascades as a result of SARM abuse can be a risk factor for the development of various cardiovascular diseases. The present literature review explores the implications of the interaction between SARMs and the AR on cardiovascular health by focusing on the AR structure, function, and mechanisms of action, as well as the current clinical literature on various SARMs. It is shown that SARMs may increase the risk of cardiovascular diseases through implications on the renin–angiotensin system, smooth muscle cells, sympathetic nervous system, lipid profile, inflammation, platelet activity, and various other factors. More research on this topic is necessary as SARM abuse is becoming increasingly common. There is a noticeable lack of clinical trials and literature on the relationship between SARMs, cardiovascular diseases, and the AR. Future in vivo and in vitro studies within this field are vital to understand the mechanisms that underpin these complex interactions and risk factors.
Diet and Nutritional Status of Polish Girls with Rett Syndrome—A Case-Control Study
Authors: Czerwonogrodzka-Senczyna Aneta , Milewska Magdalena , Kwiecień Paweł , Szczałuba Krzysztof
Published: July 2023

(1) Background: Rett syndrome may be considered a disease strongly associated with nutritional disorders that are likely to require special management strategies, extending beyond what is usually required for children with other developmental disorders. The aim of the study was to assess the nutritional status and diet of Polish girls with Rett syndrome. (2) Methods: Each patient (study group = 49, control group = 22) underwent anthropometric measurements, including body weight and height, waist, hip and arm circumference, and skinfold measurement. The assessment of the diet was based on the analysis of 7-day menus and the Food Frequency Questionnaire (FFQ-6). Data were analyzed using Statistica 13.3. (3) Results: The majority of the girls with Rett syndrome were deficient in weight and height, and consumed fewer calories, less protein, dietary fiber, calcium, and iron than the control group. They also drank less fluid. Soft products that were easy to chew and considered to be high in energy value were significantly more common in the menus. (4) Conclusions: Girls with Rett syndrome are characterized by weight deficiencies, poor growth that deteriorates with age, and are at risk of food shortages. Various nutritional intervention strategies should be explored to reduce and, if possible, prevent malnutrition and cachexia in such patients.
Establishment of a Nipah Virus Disease Model in Hamsters, including a Comparison of Intranasal and Intraperitoneal Routes of Challenge
Authors: Findlay-Wilson Stephen , Flett Lucy , Salguero Francisco J. , Ruedas-Torres Ines , Fotheringham Susan , Easterbrook Linda , Graham Victoria , Dowall Stuart
Published: July 2023

Nipah virus (NiV) is an emerging pathogen that can cause severe respiratory illness and encephalitis in humans. The main reservoir is fruit bats, distributed across a large geographical area that includes Australia, Southeast Asia, and Africa. Incursion into humans is widely reported through exposure of infected pigs, ingestion of contaminated food, or through contact with an infected person. With no approved treatments or vaccines, NiV poses a threat to human public health and has epidemic potential. To aid with the assessment of emerging interventions being developed, an expansion of preclinical testing capability is required. Given variations in the model parameters observed in different sites during establishment, optimisation of challenge routes and doses is required. Upon evaluating the hamster model, an intranasal route of challenge was compared with intraperitoneal delivery, demonstrating a more rapid dissemination to wider tissues in the latter. A dose effect was observed between those causing respiratory illness and those resulting in neurological disease. The data demonstrate the successful establishment of the hamster model of NiV disease for subsequent use in the evaluation of vaccines and antivirals.
Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important Bacteria
Authors: Espitia-Almeida Fabián , Valle-Molinares Roger , Navarro Quiroz Elkin , Pacheco-Londoño Leonardo C. , Galán-Freyle Nataly J.
Published: July 2023

The growing emergence of microbes resistant to commercially available antibiotic therapies poses a threat to healthcare systems worldwide. Multiple factors have been associated with the increasing incidence of hospital-acquired infections caused by antibiotic-resistant pathogens, including the indiscriminate use of broad-spectrum antibiotics, the massive application of antibiotics in hospitals as a prophylactic measure, self-medication, and nonadherence to pharmacological therapies by patients. In this study, we developed a novel treatment to mitigate the impact of microbial resistance. We synthesized a benzoporphyrin derivative, 5,10,15,20-tetrakis (4-ethylphenyl) porphyrin (TEtPP), with a reaction yield close to 50%. TEtPP exhibited excellent photophysical properties (Φf = 0.12 ± 0.04 and ΦΔ = 0.81 ± 0.23) and was thereby assessed as a potential agent for antibacterial photodynamic therapy. The photophysical properties of the synthesized porphyrin derivative were correlated with the assayed antimicrobial activity. TEtPP showed higher activity against the MRSA strain under irradiation than in the absence of irradiation (minimum inhibitory concentration (MIC) = 69.42 µg/mL vs. MIC = 109.30 µg/mL, p < 0.0001). Similar behavior was observed against P. aeruginosa (irradiated MIC = 54.71 µg/mL vs. nonirradiated MIC = 402.90 µg/mL, p < 0.0001). TEtPP exhibited high activity against S. aureus in both the irradiated and nonirradiated assays (MIC = 67.68 µg/mL vs. MIC = 58.26 µg/mL, p = 0.87).
Development of Diclofenac Sodium 3D Printed Cylindrical and Tubular-Shaped Tablets through Hot Melt Extrusion and Fused Deposition Modelling Techniques
Authors: Digkas Tryfon , Porfire Alina , Van Renterghem Jeroen , Samaro Aseel , Borodi Gheorghe , Vervaet Chris , Crișan Andrea Gabriela , Iurian Sonia , De Beer Thomas , Tomuta Ioan
Published: July 2023

The present study aimed to develop 3D printed dosage forms, using custom-made filaments loaded with diclofenac sodium (DS). The printed tablets were developed by implementing a quality by design (QbD) approach. Filaments with adequate FDM 3D printing characteristics were produced via hot melt extrusion (HME). Their formulation included DS as active substance, polyvinyl alcohol (PVA) as a polymer, different types of plasticisers (mannitol, erythritol, isomalt, maltodextrin and PEG) and superdisintegrants (crospovidone and croscarmellose sodium). The physicochemical and mechanical properties of the extruded filaments were investigated through differential scanning calorimetry (DSC), X-ray diffraction (XRD) and tensile measurements. In addition, cylindrical-shaped and tubular-shaped 3D dosage forms were printed, and their dissolution behaviour was assessed via various drug release kinetic models. DSC and XRD results demonstrated the amorphous dispersion of DS into the polymeric filaments. Moreover, the 3D printed tablets, regardless of their composition, exhibited a DS release of nearly 90% after 45 min at pH 6.8, while their release behaviour was effectively described by the Korsmeyer–Peppas model. Notably, the novel tube design, which was anticipated to increase the drug release rate, proved the opposite based on the in vitro dissolution study results. Additionally, the use of crospovidone increased DS release rate, whereas croscarmellose sodium decreased it.
Antibody–Drug Conjugates: Ushering in a New Era of Cancer Therapy
Authors: Hurwitz Joshua , Haggstrom Lucy Roxana , Lim Elgene
Published: July 2023

Antibody–drug conjugates (ADCs) have provided new therapeutic options and significant promise for patients with cancer, particularly where existing treatments are limited. Substantial effort in ADC development is underway globally, with 13 ADCs currently approved and many more in development. The therapeutic benefits of ADCs leverage the ability to selectively target cancer cells through antibody binding, resultant relative sparing of non-malignant tissues, and the targeted delivery of a cytotoxic payload. Consequently, this drug class has demonstrated activity in multiple malignancies refractory to standard therapeutic options. Despite this, limitations exist, including narrow therapeutic windows, unique toxicity profiles, development of therapeutic resistance, and appropriate biomarker selection. This review will describe the development of ADCs, their mechanisms of action, pivotal trials, and approved indications and identify common themes. Current challenges and opportunities will be discussed for this drug class in cancer therapeutics at a time when significant developments in antibody therapies, immunotherapy, and targeted agents are occurring.
Station Arrangement Optimization of Photoelectric Theodolites Based on Efficient Traversing Discrete Points
Authors: Miao Zhenyu , Li Yaobin , Wang Chong , Yu Yi , Liu Zhenyu
Published: July 2023

Station arrangement optimization of photoelectric theodolites in shooting ranges presents a non-convex and non-linear problem, and the method required to seek the global optimal solution remains an open question. This paper proposes an efficient traversal algorithm that could solve this problem by utilizing discretization of regions with a finite length of mesh, in which both the terrain of the station arrangement region and the observation airspace region are discretized through triangulation. To enhance the computational efficiency of the traversal algorithm, two strategies are employed to speed up the calculation: reducing the dimension of the observation airspace and using the Euclidean distance matrix to compute the intersection angle. After the global optimal solution with discrete finite precision was obtained through the traversal algorithm, it was then used as the initial points for local mesh refinement and to implement gradient-based optimization in order to further improve the precision of the solution. The proposed approach is demonstrated to be practical through application to numerical examples used for the optimization of station arrangements that involve two to four stations.
Adaptative Strategies in Gymnocalycium Species (Cactaceae) and the Presence of Ectomycorrhizae Associated with Survival in Arid Environments
Authors: Soto Acosta María E. , Perea Mario , Ruiz Ana I. , Hilal Mirna , Albornoz Patricia L. , Isla María I.
Published: July 2023

The Cactaceae family makes use of different strategies, both physiological and biochemical, for anatomical adjustments that allow them to grow and reproduce in arid environments. Morphological studies of Gymnocalycium have been scarce, and the anatomy and phytochemistry are still largely unknown. The aim of the present work was to analyze the structural, physiological, and biochemical features of Gymnocalycium marianae and G. oenanthemum, two endemic species of arid regions in Argentina. The anatomic structure, biomass, and photosynthetic pigments, as well as phenolic compound contents, were analyzed in the stem, spine, and root of both species. G. marianae showed stems with deeper substomatal chambers and a more developed photosynthetic tissue than G. oenanthemum. The spines of G. oenanthemum showed higher biomass, thicker epidermal and subepidermal cell walls, and a higher content of phenolic compounds than those of G. marianae. Ectomycorrhizae were observed for the first time in roots in both species. Roots of G. marianae showed high colonization, biomass, and content of phenolic compounds. Both species showed abundant mucilaginous fibers in the stem and root. Finally, these results show the strategies associated with the survival in xeric environments of two cacti species at risk of extinction. They could be useful for the development of ex situ conservation programs.
Application of High-Intensity Ultrasound on Acerola (Malpighia emarginata) Juice Supplemented with Fructooligosaccharides and Its Effects on Vitamins, Phenolics, Carotenoids, and Antioxidant Capacity
Authors: Fernandes Fabiano A. N. , Santos Valéria O. , Gomes Wesley F. , Rodrigues Sueli
Published: July 2023

Acerola is considered a superfruit, rich in vitamin C, phenolics, and carotenoids, and having a high antioxidant capacity. However, it is poor in oligosaccharides. Ultrasound technology can improve the bioavailability of several bioactive compounds, improving the nutritional content of several fruit juices. This work evaluated the use of ultrasound processing on acerola juice supplemented with fructooligosaccharides (FOS; 1% w/w) and its effects on the availability of vitamins, carotenoids, and phenolic content. The antioxidant capacity of the juice was correlated with its bioactive contents. The study evaluated the effects of important sonication parameters, such as ultrasonic power density, processing time, and processing temperature. The application of ultrasound was efficient in increasing the availability of some vitamins. As a result, ultrasound application increased the availability of vitamins B1, B3, B5, C, carotenoids, and phenolic compounds. This improvement increased the antioxidant activity of the FOS-enriched acerola juice.
Material Transport and Flow Pattern Characteristics of Gas–Liquid–Solid Mixed Flows
Authors: Chen Juntong , Ge Man , Li Lin , Zheng Gaoan
Published: July 2023

Flow pattern monitoring of gas–liquid–solid mixed flow has great significance to enhance the quality and efficiency of material mixing, and the material transport mechanism and dynamic control strategy are faced with significant challenges. To solve these problems, a computational fluid mechanics and discrete element method (CFD-DEM) coupling modeling and solving approach based on soft sphere and porous models is presented to explore material transport mechanisms. The user-defined function (UDF) is adopted to perform data communication, and the porosity of the porous model is calculated to achieve the bidirectional calculation of Eulerian fluid and Lagrange particle phases. Material transport processes of gas–liquid–solid mixed flows are discussed to explore material transport mechanisms of particle flow and the flow pattern evolution laws under the inflation control are obtained. The results show that the particles are not evenly distributed under the synergistic action of impeller rotation and inflation. The particles in the upper and lower impeller have similar characteristics along the radial direction, and there is an aggregation phenomenon in the impeller center. A certain degree of inflation enhances the macroscopic mixing process of turbulent vortices, promotes the particle suspension effect inside the container, and improves the material transport efficiency inside the mixing space. Relevant research results can provide theoretical references for the material transport mechanism, flow pattern tracking models, and energy transfer and can also provide technical support for chemical process separation, food processing, battery homogenate mixing, and other production processes.
Green Extraction Techniques of Bioactive Compounds: A State-of-the-Art Review
Authors: Martins Rodrigo , Barbosa Ana , Advinha Bárbara , Sales Hélia , Pontes Rita , Nunes João
Published: July 2023

Green extraction techniques are more and more relevant due to major sustainable goals set by the United Nations. Greener extraction processes are being designed through the use of unconventional extraction techniques and green solvents, resulting in less hazardous processes which, consequently, reduces environmental impacts. This is also in line with the main principles of green chemistry. Additionally, greener extraction techniques intend to solve different drawbacks that are often related to conventional extraction techniques such as the high environmental impact. Biorefineries are a major player in developing greener extraction processes. These facilities take full advantage of several biomass sources, such as food waste, microalgae, and lignocellulosic biomass, in order to create high-value products, energy, alternative fuels, and bioactive compounds. Herein, a state-of-the-art review is presented, focused on presenting the greenest and least hazardous extraction processes that have been reported on the main biomass sources of a biorefinery—food waste, microalgae, and lignocellulosic biomass. Bioactive compounds such as phenolic compounds, bioactive pigments, and fatty acids are important in several sectors, mainly, the health, pharmaceutical, and agro-food sectors. Moreover, the bioactive compounds obtained through the aforementioned biomass sources and the different extraction procedures used will be presented and the authors will attempt to discuss, compare, and provide information about the most effective extraction techniques for each compound. Therewith, this review article should serve as a guide for industries, academics, and biorefineries in the future development of optimized and greener extraction procedures. Such analysis is lacking and could be very helpful for future research biorefinery projects since it tackles all of the major biomass sources of a biorefinery in a review article. To the best of our knowledge, this brings a novelty to the scientific community.
A Two-Stage Optimal Preventive Control Model Incorporating Transient Stability Constraints in the Presence of Multi-Resource Uncertainties
Authors: Ni Qiulong , Sun Jingliao , Zha Xianyu , Zhou Taibin , Sun Zelun , Zhao Ming
Published: July 2023

The volatility and uncertainty introduced by increasingly integrated renewable energy pose challenges to the reliable and stable operation of the power system. To mitigate the operation risks, a two-stage optimal preventive control model that incorporates transient stability constraints and considers uncertainties from multiple resources is proposed. First, the uncertainties of different re-sources are modeled, with which the non-sequential Monte Carlo sampling method is used to correspondingly generate the scenarios. Thereafter, a two-stage control model that balances operational safety and economy and realizes preventive control and emergency control is built. The operation schedule from the preventive control stage aims to minimize the transient stability probability and operation costs. If any faults destabilize the system, the emergency control stage will be activated immediately to help the system recover stability with minimal control costs. To expedite the solving of the two-stage model, a multi-objective particle swarm algorithm based on entropy-TOPSIS is proposed. Finally, the effectiveness of the proposed model and solving algorithm are validated with the modified IEEE118 node system.
The Effect of COVID-19 Transmission on Cryptocurrencies
Authors: Dardouri Nesrine , Aguir Abdelkader , Smida Mounir
Published: July 2023

In recent years, Bitcoin and other cryptocurrencies like Ethereum and Dogecoin have emerged as important asset classes in general, and diversification and hedging instruments in particular. The recent COVID-19 pandemic has provided the chance to examine and assess cryptocurrencies’ behavior during extremely stressful times. The methodology of this study is based on an estimate using the ARDL model from 22 January 2020 to 12 March 2021, allowing us to analyze the long-term and short-term relationship between cryptocurrencies and COVID-19. Our results demonstrate that there is cointegration between the chosen cryptocurrencies in the market and COVID-19. The results indicate that Bitcoin, ETH, and DOGE prices were affected by COVID-19, which means that the pandemic seriously affected the three cryptocurrency prices.
Evaluation of Five Global Top-of-Atmosphere Outgoing Longwave Radiation Products
Authors: Zhan Chuan , Yang Jing , Li Yan , Chen Yong , Miao Zuohua , Zeng Xiangyang , Li Jun
Published: July 2023

Five global monthly top-of-atmosphere (TOA) outgoing longwave radiation (OLR) products are evaluated in this study, including the products derived from the High-Resolution Infrared Radiation Sounder (HIRS), Clouds and the Earth’s Radiant Energy System (CERES), Advanced Very High Resolution Radiometer (AVHRR), the CM SAF cLoud, Albedo and surface RAdiation dataset from AVHRR data (CLARA), and the Global Energy and Water Cycle EXchanges (GEWEX) project. Results show that overall there is good consistency among these five products. Larger differences are found between GEWEX and CERES (HIRS) after (before) 2000 (RMSE ~ 5 W/m2), particularly in the tropical regions. In terms of global mean values, GEWEX shows large differences with the other products from the year 1992 to 2002, and CLARA shows large differences from the year 1979 to 1981, which are more obvious in the global ocean values. Large discrepancies among these products exist at low latitudinal bands, particularly before the year 2000. Australia and Asia (mid–low latitude part) are two typical regions in which larger differences are found.
Retrieval of Sea Surface Skin Temperature from the High Resolution Picture Transmission Data of the National Oceanic and Atmospheric Administration Series Satellites
Authors: Chen Yan , Qu Liqin , Li Zhuomin , Guan Lei
Published: July 2023

The High Resolution Picture Transmission (HRPT) data of the National Oceanic and Atmospheric Administration (NOAA) series meteorological satellites had been received by the SeaSpace ground station located at the Ocean University of China (OUC). Based on the atmospheric radiative transfer model, we obtained the NOAA-15/16/17/18/19 Advanced Very High Resolution Radiometer (AVHRR) sea surface skin temperature (SSTskin) data using the Bayesian cloud detection method and the optimal estimation (OE) sea surface temperature (SST) retrieval algorithm. Compared with the NOAA/AVHRR multi-channel SST data, the AVHRR SSTskin data have higher data accuracy. We also compared the AVHRR SSTskin with the buoy SST with spatial and temporal windows of 0.01° and 30 min. The daytime biases ranged from −0.32 °C (NOAA-16) to 0.08 °C (NOAA-17) with standard deviations (SDs) ranging from 0.36 °C (NOAA-18/ NOAA-19) to 0.60 °C (NOAA-16), and the nighttime biases ranged from −0.26 °C (NOAA-16) to −0.02 °C (NOAA-17) with SDs ranging from 0.33 °C (NOAA-19) to 0.60 °C (NOAA-16). The accuracy of all five satellite data during daytime and nighttime was significantly improved. These results show that the AVHRR SSTskin of NOAA series satellites is good and consistent in different periods, and the SSTskin data products with high spatial resolution and accuracy can be used for mesoscale and submesoscale marine applications.
A LiDAR-Camera-Inertial-GNSS Apparatus for 3D Multimodal Dataset Collection in Woodland Scenarios
Authors: Cristóvão Mário P. , Portugal David , Carvalho  Afonso E. , Ferreira  João Filipe
Published: July 2023

Forestry operations have become of great importance for a sustainable environment in the past few decades due to the increasing toll induced by rural abandonment and climate change. Robotics presents a promising solution to this problem; however, gathering the necessary data for developing and testing algorithms can be challenging. This work proposes a portable multi-sensor apparatus to collect relevant data generated by several onboard sensors. The system incorporates Laser Imaging, Detection and Ranging (LiDAR), two stereo depth cameras and a dedicated inertial measurement unit (IMU) to obtain environmental data, which are coupled with an Android app that extracts Global Navigation Satellite System (GNSS) information from a cell phone. Acquired data can then be used for a myriad of perception-based applications, such as localization and mapping, flammable material identification, traversability analysis, path planning and/or semantic segmentation toward (semi-)automated forestry actuation. The modular architecture proposed is built on Robot Operating System (ROS) and Docker to facilitate data collection and the upgradability of the system. We validate the apparatus’ effectiveness in collecting datasets and its flexibility by carrying out a case study for Simultaneous Localization and Mapping (SLAM) in a challenging woodland environment, thus allowing us to compare fundamentally different methods with the multimodal system proposed.
Vector Angular Continuity in the Fusion of Coseismic Deformations at Multiple Optical Correlation Scales
Authors: Guo Rui , Zeng Qiming , Lu Shangzong
Published: July 2023

As one of the common techniques for measuring coseismic deformations, optical image correlation techniques are capable of overcoming the drawbacks of inadequate coherence and phase blurring which can occur in radar interferometry, as well as the problem of low spatial resolution in radar pixel offset tracking. However, the scales of the correlation window in optical image correlation techniques typically influence the results; the conventional SAR POT method faces a fundamental trade-off between the accuracy of matching and the preservation of details in the correlation window size. This study regards coseismic deformation as a two-dimensional vector, and develops a new post-processing workflow called VACI-OIC to reduce the dependence of shift estimation on the size of the correlation window. This paper takes the coseismic deformations in both the east–west and north–south directions into account at the same time, treating them as vectors, while also considering the similarity of displacement between adjacent points on the surface. Herein, the angular continuity index of the coseismic deformation vector was proposed as a more reasonable constraint condition to fuse the deformation field results obtained by optical image correlation across different correlation window. Taking the earthquake of 2021 in Maduo, China, as the study area, the deformation with the highest spatial resolution in the violent surface rupture area was determined (which could not be provided by SAR data). Compared to the results of single-scale optical correlation, the presented results were more uniform (i.e., more consistent with published results). At the same time, the proposed index also detected the strip fracture zone of the earthquake with impressive clarity.
Determining the Elastic Constants of Isotropic Materials by Measuring the Phase Velocities of the A0 and S0 Modes of Lamb Waves
Authors: Tumšys Olgirdas , Mažeika Liudas
Published: July 2023

In this study, a new method for determining the elastic constants of isotropic plates using Lamb wave fundamental modes is presented. This method solves the inverse problem, where the elastic constants (Young’s modulus and Poisson’s ratio) of the plate were estimated by measuring the phase velocities of the Lamb wave using the Rayleigh–Lamb equations to find the solution and determining the phase velocities of the A0 and S0 modes using a new method. The suitability of the proposed method for determining the elastic constants was evaluated using simulated and experimental signals propagating on an aluminum plate. The theoretical modeling on the aluminum 7075-T6 plate shows that the proposed method allows the determination of the Poisson ratio with a relative error not exceeding 2% and Young’s modulus with a relative error not exceeding 0.5%. The experimental measurements of an aluminum plate of known thickness (2 mm) and density (2685 kg/m3) confirmed the suitability of the proposed method for the measurements of elastic constants. In the proposed method, the processing of ultrasonic signals can be performed in real-time, and the values of the elastic constants can be obtained immediately after scanning the required distance.
A Real-Time Evaluation Algorithm for Noncontact Heart Rate Variability Monitoring
Authors: Han Xiangyu , Zhai Qian , Zhang Ning , Zhang Xiufeng , He Long , Pan Min , Zhang Bin , Liu Tao
Published: July 2023

Noncontact vital sign monitoring based on radar has attracted great interest in many fields. Heart Rate Variability (HRV), which measures the fluctuation of heartbeat intervals, has been considered as an important indicator for general health evaluation. This paper proposes a new algorithm for HRV monitoring in which frequency-modulated continuous-wave (FMCW) radar is used to separate echo signals from different distances, and the beamforming technique is adopted to improve signal quality. After the phase reflecting the chest wall motion is demodulated, the acceleration is calculated to enhance the heartbeat and suppress the impact of respiration. The time interval of each heartbeat is estimated based on the smoothed acceleration waveform. Finally, a joint optimization algorithm was developed and is used to precisely segment the acceleration signal for analyzing HRV. Experimental results from 10 participants show the potential of the proposed algorithm for obtaining a noncontact HRV estimation with high accuracy. The proposed algorithm can measure the interbeat interval (IBI) with a root mean square error (RMSE) of 14.9 ms and accurately estimate HRV parameters with an RMSE of 3.24 ms for MEAN (the average value of the IBI), 4.91 ms for the standard deviation of normal to normal (SDNN), and 9.10 ms for the root mean square of successive differences (RMSSD). These results demonstrate the effectiveness and feasibility of the proposed method in emotion recognition, sleep monitoring, and heart disease diagnosis.
Interference Level Detector with PCB HDI Rogowski coil for PLC Narrow-Band Applications
Authors: Lisowiec Aleksander , Habrych Marcin , Michalski Pawel , Miedzinski Bogdan , Wisniewski Grzegorz
Published: July 2023

This article presents and discusses the structure, principle of operation, and operational properties of a newly developed interference level detector (ILD) designed to measure conducted supraharmonic disturbances (1–150 kHz) in the power grid and to assess the effectiveness of narrow-band Power Line Communication (PLC) transmission, especially in the PRIME technology. The usability assessment was made on the basis of the validation and the results of tests carried out in a low-voltage network with non-linear loads. Appropriate practical conclusions were then formulated.
Optical Multi-Parameter Measuring System for Fluid and Air Bubble Recognition
Authors: Bello Valentina , Bodo Elisabetta , Merlo Sabina
Published: July 2023

Detection of air bubbles in fluidic channels plays a fundamental role in all that medical equipment where liquids flow inside patients’ blood vessels or bodies. In this work, we propose a multi-parameter sensing system for simultaneous recognition of the fluid, on the basis of its refractive index and of the air bubble transit. The selected optofluidic platform has been designed and studied to be integrated into automatic pumps for the administration of commercial liquid. The sensor includes a laser beam that crosses twice a plastic cuvette, provided with a back mirror, and a position-sensitive detector. The identification of fluids is carried out by measuring the displacement of the output beam on the detector active surface and the detection of single air bubbles can be performed with the same instrumental scheme, exploiting a specific signal analysis. When a bubble, traveling along the cuvette, crosses the readout light beam, radiation is strongly scattered and a characteristic fingerprint shape of the photo-detected signals versus time is clearly observed. Experimental testing proves that air bubbles can be successfully detected and counted. Their traveling speed can be estimated while simultaneously monitoring the refractive index of the fluid.
A Portable Laser Spectroscopic System for Measuring Nitrous Oxide Emissions on Fertilized Cropland
Authors: Stiefvater Gerrit , Hespos Yvonne , Wiedenmann Dominic , Lambrecht Armin , Brunner Raimund , Wöllenstein Jürgen
Published: July 2023

Nitrous oxide (laughing gas, N2O) is a relevant greenhouse gas. Agriculture contributes significantly to its emissions. As nitrogen fertilization has been identified as one of the main sources of N2O, controlled application and reduction of the amount of fertilizer adapted to crop demand is essential to reduce N2O emissions. This requires detailed studies of the local distribution of the N2O emission fluxes on different croplands. Consequently, frequent spatially resolved field measurements of N2O concentrations are needed. A precision in the ppb range close to the ambient N2O level of 333 ppb is necessary. Tunable laser absorption spectroscopy using quantum-cascade lasers (QCL) as a light source is an established technique for the measurement of N2O traces. We present the development and validation of a compact portable setup for on-site measurement of N2O emissions from the soil. The setup differs from previous solutions by using an interband cascade laser (ICL), which has significantly lower power consumption compared to a QCL. The portable measurement setup allows N2O emission fluxes to be determined with a precision of 3.5% with a measuring duration of 10 min. The developed system enables the detection of increased N2O emissions because of the fertilization of fields. High N2O emission fluxes are indicators of the overfertilization of the field. Directly after fertilization, N2O fluxes between 2.9 and 5.3 µL m−2 min−1 depending on the gas acquisition site are measured during the field tests. Over time, the fluxes decrease. The obtained results compare well with data from more precise but also more complex and maintenance-intensive instruments for atmospheric research. With this system, the soil moisture as well as the air humidity and air temperature are recorded. Strong influences on N2O fluxes by soil moisture were observed. The presented measurement system is a contribution to the establishment of mobile N2O screening systems that are robust in the field and suitable for comprehensive and routine detection of N2O emissions from soil.