bluuebunny/crossref_metadata_2025 huggingface dataset:
Metagenomic assessment of the bacterial breastfeeding microbiome in mature milk across lactation
Authors: Ingram Kelly , Gregg Collin , Tegge Allison , Elison Jed T. , Lin Weili , Howell Brittany R.
Published: July 2024

Introduction

Research has illustrated the presence of a diverse range of microbiota in human milk. The composition of the milk microbiome varies across different stages of lactation, emphasizing the need to consider the lactation stage when studying its composition. Additionally, the transfer of both milk and skin microbiota during breastfeeding is crucial for understanding their collective impact on infant health and development. Further exploration of the complete breastfeeding microbiome is necessary to unravel the role these organisms play in infant development. We aim to longitudinally assess the bacterial breastfeeding microbiome across stages of lactation. This includes all the bacteria that infants are exposed to during breastfeeding, such as bacteria found within human milk and any bacteria found on the breast and nipple.

Methods

Forty-six human milk samples were collected from 15 women at 1, 4, 7, and 10 months postpartum. Metagenomic analysis of the bacterial microbiome for these samples was performed by CosmosID (Rockville, MD) via deep sequencing.

Results

Staphylococcus epidermidis and Propionibacteriaceae species are the most abundant bacterial species from these samples. Samples collected at 10 months showed higher abundances of Proteobacteria, Streptococcaceae, Lactobacillales, Streptococcus, and Neisseria mucosa compared to other timepoints. Alpha diversity varied greatly between participants but did not change significantly over time.

Discussion

As the bacterial breastfeeding microbiome continues to be studied, bacterial contributions could be used to predict and reduce health risks, optimize infant outcomes, and design effective management strategies, such as altering the maternal flora, to mitigate adverse health concerns.
Predicting postoperative pain in children: an observational study using the pain threshold Index
Authors: Liang Zenghui , Xie Yanle , Chen Shuhan , Liu Jing , Lv Huimin , Muhoza Bertrand-Geoffrey , Xing Fei , Mao Yuanyuan , Wei Xin , Xing Na , Yang Jianjun , Wang Zhongyu , Yuan Jingjing
Published: July 2024

Objective

While the pain threshold index (PTI) holds potential as a tool for monitoring analgesia-pain equilibrium, its precision in forecasting postoperative pain in children remains unconfirmed. This study's primary aim was to assess the PTI's predictive precision for postoperative pain.

Methods

Children (aged 2–16 years) undergoing general surgery under general anesthesia were included. Within 5 min prior to the patient's emergence from surgery, data including PTI, wavelet index (WLI), heart rates (HR) and mean arterial pressure (MAP) were collected. Subsequently, a 15-min pain assessment was conducted following the patient's awakening. The accuracy of these indicators in discerning between mild and moderate to severe postoperative pain was evaluated through receiver operating characteristic (ROC) analysis.

Results

The analysis encompassed data from 90 children. ROC analysis showed that PTI was slightly better than HR, MAP and WLI in predicting postoperative pain, but its predictive value was limited. The area under the curve (AUC) was 0.659 [0.537∼0.780] and the optimal threshold was 65[64–67]. Sensitivity and specificity were determined at 0.90 and 0.50, respectively. In a multivariable logistic regression model, a higher predictive accuracy was found for a multivariable predictor combining PTI values with gender, BMI, HR and MAP (AUC, 0.768; 95%CI, 0.669–0.866). Upon further scrutinizing the age groups, PTI's AUC was 0.796 for children aged 9–16, 0.656 for those aged 4–8, and 0.601 for younger individuals.

Conclusions

PTI, when used alone, lacks acceptable accuracy in predicting postoperative pain in children aged 2 to 16 years. However, when combined with other factors, it shows improved predictive accuracy. Notably, PTI appears to be more accurate in older children.
Exploring the associations between elevated plasma SP-D levels and OSCAR gene expression as potential biomarkers in patients with COPD: a cross-sectional study
Authors: Mukhtar Saima , Choudhry Nakhshab , Saeed Saqib , Hanif Asif , Gondal Aamir J. , Yasmin Nighat
Published: July 2024

Background: Chronic obstructive pulmonary disease (COPD) imposes a substantial burden on patients and healthcare systems. Spirometry is the most widely used test to diagnose the disease; however, a surrogate marker is required to predict the disease pattern and progression.

Objectives: The aim of the current study was to explore the association of elevated levels of plasma surfactant protein D (SP-D) with gene expression of osteoclast-associated receptor (OSCAR) and lung functions as potential diagnostic biomarkers of COPD.

Methods: This cross-sectional study employed convenience sampling. As men compose the majority of patients in the outpatient department and with smoking being common among Pakistani men, choosing men offered a representative sample. Using a post-bronchodilator forced expiratory volume in the first second (FEV1) to a forced vital capacity (FVC) of less than 0.70 (FEV1/FVC <0.7), COPD patients were diagnosed on spirometry (n = 41). Controls were healthy individuals with FEV1/FVC >0.7 (n = 41). Plasma SP-D levels were measured using an enzyme-linked immunosorbent assay (ELISA). The gene expression of OSCAR was determined by real-time polymerase chain reaction (qPCR) and subsequently analyzed by the threshold cycle (Ct) method. Statistical Package for Social Sciences (SPSS) version 20 was used for statistical analysis.

Results: The mean BMI of controls (25.66 ± 4.17 kg/m2) was higher than that of cases (23.49 ± 2.94 kg/m2 (p = .008)). The median age of controls was 49 years (interquartile range (IQR) 42.0–65.0 years) and that of cases was 65 years (IQR = 57.50–68.50). SP-D concentration was not significantly higher in COPD patients [4.96 ng/mL (IQR 3.26–7.96)] as compared to controls [3.64 ng/mL (IQR 2.60–8.59)] (p = .209). The forced expiratory ratio (FEV1/FVC) and FEV1 were related to gene expression of OSCAR (p = <.001). The gene expression of OSCAR was significantly related to SP-D (p = .034). A multiple regression model found FEV1 and FVC to have a significant effect on the gene expression of OSCAR (p-values <0.001 and 0.001, respectively).

Conclusion: Gene expression of OSCAR was increased in COPD patients and related directly to SP-D levels and inversely to lung functions in cohort of this study, suggesting that OSCAR along with SP-D may serve as a diagnostic biomarker of COPD.
Impact of carboxylic acid structure on α-hemihydrate gypsum crystal morphology and mechanical strength
Authors: Li Guo-gang , Liu Jin-e , Ma Liang , Gong Hao-lei , Yin Su-hong
Published: July 2024

This study investigated the synthesis of α-hemihydrate gypsum (α-HH) through semi-liquid autoclaving of phosphogypsum (PG) using various carboxylic acids as modifying agents. The impact of carboxyl group spatial location, auxiliary functional group type, and the number of carboxyl groups within the carboxylic acid modifiers on the mechanical strength and crystal morphology of α-HH was analyzed using scanning electron microscopy (SEM), strength testing, and molecular dynamics simulations. The results revealed a significant influence of the carboxylic acid molecular structure on the α-HH crystal morphology. Monocarboxylic acids and dicarboxylic acids with a long carbon chain length between carboxyl groups exhibited preferential adsorption on the (200), (110), and (−110) crystal planes, promoting crystal growth along the c-axis. In contrast, hydroxyl groups and cis double bonds in the modifier structure induced selective adsorption on the (001) plane, hindering growth along the c-axis. Conversely, trans double bonds favored adsorption on the (200), (110), and (−110) planes, enhancing growth along the c-axis. Based on these observations, screening principles for carboxylic acid modifiers were established, suggesting that: 1) the number of carboxyl groups should exceed 2; 2) the optimal carbon atom spacing between carboxyl groups is 3; and 3) auxiliary functional groups such as hydroxyl groups and cis double bonds should be introduced. Modifiers like citric acid, ethylene diamine tetraacetic acid (EDTA), and pyromellitic acid, within concentration ranges of 0.05%–0.1%, 0.1%–0.15%, and 0.05%–0.1%, respectively, yielded α-HH with flexural strengths exceeding 4 MPa and compressive strengths greater than 35 MPa, demonstrating the validity of these principles.
E. coli-expressed SECRET AGENT O-GlcNAc modifies threonine 829 of GIGANTEA
Authors: Kim Young-Cheon , Hartweck Lynn M. , Olszewski Neil E.
Published: July 2024

The Arabidopsis thaliana glycosyl transferases SPINDLY (SPY) and SECRET AGENT (SEC) modify nuclear and cytosolic proteins with O-linked fucose or O-linked N-acetylglucosamine (O-GlcNAc), respectively. O-fucose and O-GlcNAc modifications can occur at the same sites. SPY interacts physically and genetically with GIGANTEA (GI), suggesting that it could be modified by both enzymes. Previously, we found that, when co-expressed in Escherichia coli, SEC modifies GI; however, the modification site was not determined. By analyzing the overlapping sub-fragments of GI, we identified a region that was modified by SEC in E. coli. Modification was undetectable when threonine 829 (T829) was mutated to alanine, while the T834A and T837A mutations reduced the modification, suggesting that T829 was the primary or the only modification site. Mapping using mass spectrometry detected only the modification of T829. Previous studies have shown that the positions modified by SEC in E. coli are modified in planta, suggesting that T829 is O-GlcNAc modified in planta.
Bacterial community structure of Physalis peruviana L. fruit exocarp and the presence of pathogens with possible implications on food safety
Authors: Tenea Gabriela N. , Molina Diana
Published: July 2024

Introduction

Cape gooseberry (Physalis peruviana L.) is a wellconsumed crop in Ecuador, whose fruits are abundant in bioactive molecules. Its rapid post-harvest deterioration and safety limit its market potential.

Methodology

To gather baseline data on the prevalence of bacterial taxa among groups, we employed 16S ribosomal RNA (16S rRNA) amplicon gene sequencing to detect changes in the bacterial community structure in cape gooseberry fruits harvested from an organic farm production system (# 270 samples x two ripeness stages), and fruits obtained from an open-air market (#270).

Results

This is the first report of bacterial taxa inhabiting cape gooseberry fruits. Shannon’s diversity index revealed that the fruits purchased from the market and the unripe stage had the highest level of bacterial diversity (average Shannon indices of 3.3 and 3.1) followed by those collected from the field at the mature ripe stage (2.07). Alpha diversity analysis indicated that there were no significant differences in the number of taxa or evenness within the sample, whereas there was a significant difference in beta diversity between the groups. Rhizobiaceae was the most abundant family in fruits originating from the field regardless of the ripe stage, while Acetobacteraceae, Pseudomonadaceae, Fusobacteriaceae, Bacteroidaceae, and Erwiniaceae were the most abundant families in the market group. At the genus level, Liberibacter was the most abundant phytopathogen in fruits originating from the field, while Gluconobacter was the most abundant in samples collected from the market. The phytopathogen Candidatus_Liberibacter was the most abundant in samples collected from the field, while the fruits purchased from the market stands contained opportunistic enteric pathogens such as Escherichia vulneris, Klebsiella pneumoniae, and K. variicola, their relative abundance varied with the sample. In addition, potential pathogens of animal origin such as Fusobacterium necrophorum, Porphyromonas levii, Helcococcus ovis, and Trueperella pyogenes were found in almost all samples at varying relative abundance.

Conclusion

Our study provides basic information on the microbiome of cape gooseberries from agriculture fields to the table along with the detection of several pathogenic microorganisms with possible impact on food safety and public health therefore, strategies for reducing bacterial contamination in both farm and retail markets are compulsory.
Potato soup: analysis of cultivated potato gene bank populations reveals high diversity and little structure
Authors: Tuttle Heather K. , Del Rio Alfonso H. , Bamberg John B. , Shannon Laura M.
Published: July 2024

Cultivated potatoes are incredibly diverse, ranging from diploid to pentaploid and encompass four different species. They are adapted to disparate environments and conditions and carry unique alleles for resistance to pests and pathogens. Describing how diversity is partitioned within and among these populations is essential to understanding the potato genome and effectively utilizing landraces in breeding. This task is complicated by the difficulty of making comparisons across cytotypes and extensive admixture within section petota. We genotyped 730 accessions from the US Potato genebank including wild diploids and cultivated diploids and tetraploids using Genotype-by-sequencing. This data set allowed us to interrogate population structure and diversity as well as generate core subsets which will support breeders in efficiently screening genebank material for biotic and abiotic stress resistance alleles. We found that even controlling for ploidy, tetraploid material exhibited higher observed and expected heterozygosity than diploid accessions. In particular groupchilotanummaterial was the most heterozygous and the only taxa not to exhibit any inbreeding. This may in part be because groupchilotanumhas a history of introgression not just from wild species, but landraces as well. All groupchilotanum, exhibits introgression from groupandigenumexcept clones from Southern South America near its origin, where the two groups are not highly differentiated. Moving north, we do not observe evidence for the same level of admixture back into groupandigenum. This suggests that extensive history of admixture is a particular characteristic ofchilotanum.
Laughing your vote off: the impact of candidates’ humor on voters’ emotions and intentions
Authors: Mendiburo-Seguel Andrés , Olah Andrew R. , Paez Dario , Navia Patricio
Published: July 2024

Both the Theory of Affective Intelligence and the Expectancy Violation Theory suggest emotions are crucial in supporting political candidates. However, despite the considerable existing evidence on this topic, the impact of humor-induced emotions has been less explored. In this study, we examine whether positive and negative emotions mediate the effects of politicians’ use of affiliative humor on their likeability and citizen’s intention to vote for them. To do this, we designed an experiment in the context of the Chilean presidential elections of 2021, where two candidates representing extreme opposing ideologies competed for the office of president: Gabriel Boric, from a coalition including the Communist Party, and José Antonio Kast from the extreme-right Republican Party. Both candidates used different campaign techniques, including humor that did not always have political content. The experiment was run prior to the second round of the election with a sample of Chilean voters two months before the election (n = 1,033) and had four conditions: two experimental groups watched a video of a candidate using nonpolitical humor, and two control groups did not. Results indicate that humor boosted both candidates’ likeability and vote probability differently: for Boric, it increased positive emotions; for Kast, it reduced negative emotions. Perceptions of a candidate’s humor predicted higher likeability and declared probability of voting for him, independent of political stance. We discuss these outcomes through ITA and EVT frameworks and their significance for political communication.
Individual characteristics in arts management careers: investigating the highly sensitive person scale on motivation to lead
Authors: Farstad Christian Winther , Arnulf Jan Ketil
Published: July 2024

Research on personality in leadership indicates that self-selection to leadership careers and artistic careers correlates with diverging personality profiles. People in leadership careers traditionally display lower neuroticism and higher conscientiousness than artistic individuals. In between, there are individuals entering arts management careers. To study these individuals directly, we collected Norwegian data from 91 musical theater students and 102 arts management students and compared with 109 business management students. As expected, conscientiousness and neuroticism predicted artistic careers against business management careers, aligned with the “arts for arts’ sake” myth of artists. Interestingly, arts management careers were not different from artistic careers. They weren’t more motivated to take on leadership roles than performing artists either. However, the Highly Sensitive Person Scale indicated that narrower traits of sensitivity predicted higher levels of motivation to lead in many artists. Some arts and arts management students seem to bring unique talents into forms of leadership particularly useful for artistic organizations. Our findings are discussed in terms of how leadership characteristics operate in the field of art, and the effect of domain-specific characteristics in this setting.
Unconscious multisensory integration: behavioral and neural evidence from subliminal stimuli
Authors: Frumento Sergio , Preatoni Greta , Chee Lauren , Gemignani Angelo , Ciotti Federico , Menicucci Danilo , Raspopovic Stanisa
Published: July 2024

Introduction

The prevailing theories of consciousness consider the integration of different sensory stimuli as a key component for this phenomenon to rise on the brain level. Despite many theories and models have been proposed for multisensory integration between supraliminal stimuli (e.g., the optimal integration model), we do not know if multisensory integration occurs also for subliminal stimuli and what psychophysical mechanisms it follows.

Methods

To investigate this, subjects were exposed to visual (Virtual Reality) and/or haptic stimuli (Electro-Cutaneous Stimulation) above or below their perceptual threshold. They had to discriminate, in a two-Alternative Forced Choice Task, the intensity of unimodal and/or bimodal stimuli. They were then asked to discriminate the sensory modality while recording their EEG responses.

Results

We found evidence of multisensory integration for supraliminal condition, following the classical optimal model. Importantly, even for subliminal trials participant’s performances in the bimodal condition were significantly more accurate when discriminating the intensity of the stimulation. Moreover, significant differences emerged between unimodal and bimodal activity templates in parieto-temporal areas known for their integrative role.

Discussion

These converging evidences - even if preliminary and needing confirmation from the collection of further data - suggest that subliminal multimodal stimuli can be integrated, thus filling a meaningful gap in the debate about the relationship between consciousness and multisensory integration.
Is adding resources always beneficial? Multiplicative impact of psychological capital and goal-oriented climate on Spanish public worker satisfaction and engagement
Authors: Díaz-Fúnez Pedro Antonio , Cardella Giuseppina María , Hernández-Sánchez Brizeida Raquel , Sánchez-García José Carlos , Mañas-Rodríguez Miguel Ángel
Published: July 2024

Introduction

The motivation process from personal resources to commitment of administrative employees is still little studied. This article uses the Job Demands-Resources model to investigate how the multiplicative effect of personal resources and a goal-oriented climate among public employees influences their satisfaction and engagement at work. Specifically, it proposes a model where the influence of psychological capital on engagement is mediated by job satisfaction and moderated by the goal orientation climate.

Method

A total of 326 employees of the administrative staff of a Spanish Public Administration answered a self-reported survey. Partial Least Square-Structural Equation Modeling (PLS-SEM) approach was used to evaluate the validity and reliability of the data, as well as, to test the hypotheses formulated.

Results

The SEM results show the positive impact of psychological capital on employee engagement, and the mediating role of job satisfaction in this relationship. Furthermore, the existence of a goal-oriented climate negatively moderated the relationship between Psychological Capital and Job Satisfaction, reducing the mediation effect.

Discussion

These findings open new doors for future research in the necessary adaptation of human resource policies to improve the motivation process in the public administration context.
The use of CAMS and DBT to effectively treat patients who are suicidal
Authors: Jobes David A. , Rizvi Shireen L.
Published: July 2024

Around the world, suicide ideation, attempts, and deaths pose a major public and mental health challenge for patients (and their loved ones). Accordingly, there is a clear need for effective clinical treatments that reliably reduce suicidal thoughts and behaviors. In this article, we review the Collaborative Assessment and Management of Suicidality (CAMS) and Dialectical Behavior Therapy (DBT), two clinical treatments that rise to the highest levels of empirical rigor. Both CAMS and DBT are now supported by randomized controlled trials (RCTs), with independent replications, and meta-analyses. There are also supportive data related to training clinical providers to use CAMS and DBT with adherence. RCTs that investigate the use of both interventions within clinical trial research designs and the increasing use of these complementary approaches within routine clinical practice are discussed. Future directions for research and clinical use of CAMS and DBT are explored as means to effectively treat suicidal risk.
Construction and improvement strategies of an age-friendly evaluation system for public spaces in affordable housing communities: a case study of Shenzhen
Authors: Han Jiwen , Ma Hang , Wang Mohan , Li Jinqi
Published: July 2024

Characterized by early construction periods, as the concentration of low-income populations and a high level of aging, affordable housing communities face prominent challenges such as incongruence between age-friendly construction and the needs of the older adult population. It is urgent to provide pathways and tools for identifying age-friendly issues and optimizing the built environment. The systematic evaluation of age-friendly communities serves as the foundation for implementing intervention measures by developers. Therefore, the construction of a scientifically systematic evaluation system becomes an objective necessity for age-friendly community development. Building upon existing research, this study systematically outlines the subjects, processes, methods, and content involved in constructing an age-friendly community evaluation system. By the methods such as factor analysis and analytical hierarchy process (AHP), the study focuses on the public spaces of affordable housing communities in Shenzhen as a case for constructing an age-friendly evaluation system. The empirical validation of the indicator system is conducted, and the application results are resulted into concrete improvement recommendations and action items, aiming to provide a practical, quantitative tool for community age-friendliness evaluation. The study reveals that adhering to an effective evaluation process, exploring collaborations among multiple stakeholders, determining hierarchical evaluation criteria, and adopting diversified evaluation methods are key to constructing an age-friendly evaluation system for communities. Additionally, the specificity of the evaluation system is influenced by regional demographic structures, policy backgrounds, and the built environment.
The effects of ambient particulate matter air pollution on platelets and hemostasis
Authors: Hantrakool Sasinee , Sriwichai Maitree , Shaengkhamnang Banphot , Leetrakool Nipapan , Niprapan Piangrawee , Kawichai Sawaeng , Wannakul Sitapak , Panyasit Noppamas , Tuntivate Pakinee , Wongtagan Ornkamon , Natesirinilkul Rungrote , Koonyosying Pimpisid , Phinyo Phichayut , Punnachet Teerachat , Hantrakun Nonthakorn , Piriyakhuntorn Pokpong , Rattanathammethee Thanawat , Chai-Adisaksopha Chatree , Rattarittamrong Ekarat , Tantiworawit Adisak , Norasetthada Lalita , Srichairatanakool Somdet
Published: July 2024

Introduction

Elevated ambient pollution exposure is potentially linked to thromboembolism. However, the mechanisms by which particulate matter (PM) interferes with the balance of hemostatic system remain unclear. This study investigates PM-mediated hemostatic changes in individuals across unique seasonal variations of ambient pollution.

Methods

This prospective study was conducted between February and July 2020 during alterations in ambient pollution in Chiang Mai, Thailand. Blood tests from 30 healthy subjects were assessed at four-week intervals, four times in total. Various coagulation tests, including prothrombin time (PT), activated partial thromboplastin time (aPTT), von Willebrand factor (vWF), platelet count, and platelet functions, were evaluated. A mixed-effects model was used to analyze the impact of high PM2.5 and PM10 on hemostatic parameters.

Results

Thirty male subjects with mean age of 38.9 ± 8.2 years, were included. High levels of PM2.5 and PM10 were significantly associated with PT shortening, with no such effect observed in aPTT. PM2.5 and PM10 values also positively correlated with vWF function, while vWF antigen levels remained unchanged. Soluble P-selectin showed a strong positive association with PM2.5 and PM10 levels. Platelet function analysis revealed no correlation with PM values.

Conclusion

Short-term exposure to elevated PM2.5 and PM10 concentrations was linked to shortened PT and enhanced vWF function in healthy individuals. Exploring the impact of these changes on clinically relevant thrombosis is crucial. Additional studies on the pathogenesis of pollution-related thrombosis are warranted for maintaining good health.
Exploring the relationship between physical activity and cognitive function: an fMRI pilot study in young and older adults
Authors: Feng Jie , Song Huiqi , Wang Yingying , Zhou Qichen , Zhou Chenglin , Jin Jing
Published: July 2024

Background

There are limited studies exploring the relationship between physical activity (PA), cognitive function, and the brain processing characteristics in healthy older adults.

Methods

A total of 41 participants (42.7 ± 20.5 years, 56.1% males) were included in the data analysis. The International Physical Activity Questionnaire Short Form was used to assess PA levels, and the Chinese version of the Montreal Cognitive Assessment-Basic and the Flanker task were employed to evaluate cognitive function. Furthermore, fMRI technology was utilized to examine brain activation patterns.

Results

The cognitive function of the older adults was found to be significantly lower compared to the young adults. Within the older adults, those with high levels of PA exhibited significantly higher cognitive function than those with low and medium PA levels. The fMRI data showed significant differences in brain activation patterns among young adults across the different PA levels. However, such difference was not observed among older adults.

Conclusion

A decline in cognitive function was observed among older adults. There was a significant correlation between the levels of PA and cognitive function in healthy older adults. The study demonstrated significant effects of PA levels on brain activation patterns in inhibitory control-related regions among young adults, while not significant among older adults. The findings suggest that neurological mechanisms driving the relationship between PA and cognitive function may differ between older and young adults.
Case Report: Benefits of LSVT LOUD in a multilingual patient with hypokinetic-hyperkinetic dysarthria and suspected progressive supranuclear palsy
Authors: Sebestyen Amanda , Hilger Allison , Kleiber Holly
Published: July 2024

Purpose

This case study measured how well the Lee Silverman Voice Treatment (LSVT) improved vocal features, intelligibility, and communicative effectiveness for a multilingual participant with hypokinetic-hyperkinetic dysarthria secondary to suspected progressive supranuclear palsy. LSVT treatment was chosen for the participant due to the strengths and deficits he presented with prior to treatment, and for the anticipated challenges in treatment that may arise from the presence of multilingualism and impaired cognitive functioning.

Methods

A multilingual patient in their 60's (English, Spanish, and French) with hypokinetic-hyperkinetic dysarthria secondary to suspected progressive supranuclear palsy completed the standard treatment sessions for LSVT. Assessment measures were taken at baseline, immediately post-treatment, and three-months post-treatment.

Results

Improvements were measured in vocal quality, vocal loudness, intelligibility, and communicative effectiveness immediately post-treatment. Three months post-treatment, improvements in vocal quality and intelligibility were maintained.

Conclusion

This case study illustrates that LSVT may be a beneficial treatment for complex clients who are multilingual and present with complex comorbidities and cognitive deficits. LSVT resulted in some meaningful changes in vocal quality, intelligibility, and communicative effectiveness for this individual. Clinicians who work with complex patients may wish to consider the theoretical underpinnings of LSVT, client profile, areas of client need, and ability and desire to complete an intensive treatment program to determine if trialing LSVT is appropriate. The use of LSVT with complex clients may yield positive outcomes.
The museum of digital things: extended reality and museum practices
Authors: Song Zhennuo , Evans Leighton
Published: July 2024

This study presents a philosophical reconceptualisation of museums using phenomenological frameworks. It explores the role extended reality (XR) “things” play in the museum experience and studies how their function differs from traditional objects. Existing studies highlight the technological tools, solutions and various visitor experience modalities in the museum sector. However, only a few papers focus on the theoretical aspects of using XR in museums. This empirical study adopts a qualitative research methodology, and 22 semi-structured interviews with specialists in XR design and development, museum culture, curation and museum exhibitions are conducted. The findings suggest that XR enhances museum curation and exhibition practices by providing new dimensions for interacting with museum objects. This aligns with Heidegger’s “the thing” and “fourfold” concepts—the integration of mortals (i.e., museum visitors), earth (i.e., the activities participated in by the visitors), sky (i.e., the potentialities and ideas stimulated by the artefacts) and divinities (i.e., the visitors’ emotions while interacting with the exhibits). Therefore, these new interactions, enabled by XR, can accentuate the narratives attached to and the meaning of the artefacts within the digital museum space, facilitating new understandings and relationships with history due to the digitisation of objects. Furthermore, this can potentially impact XR’s use in developing multi-channel curation and multi-sensory visitor experiences in museums and cultural heritage institutions.
Beyond the Iron Throne: exploring the representation of homosexuality in the series Game of Thrones
Authors: Louis Gerome Karthic Kumar , Chithra Gandhapodi K.
Published: July 2024

Introduction

This research delves into the representation of homosexuality in the television series Game of Thrones, adapted from George R.R. Martin's A Song of Ice and Fire saga. The study focuses on the navigation and portrayal of homosexual identities and relationships within a historically patriarchal and heteronormative genre.

Methodology

A qualitative research approach, specifically thematic analysis, was utilized to scrutinize the portrayal of homosexuality across all eight seasons and 73 episodes of Game of Thrones. The analysis encompassed pivotal scenes, character development, and dialogues related to homosexual representation.

Findings

Thematic analysis revealed several significant themes concerning the representation of homosexuality, including heteronormativity, the interplay of love and war, and the societal and personal impacts of sexual identity. The series portrays the challenges and resilience of homosexual characters, such as Renly Baratheon and Loras Tyrell, against a backdrop of political intrigue and personal ambition. However, the depiction at times veers into sensationalism, which may potentially desensitize viewers to the real-life struggles faced by LGBTQ+ individuals.

Discussion

The study findings suggest that while Game of Thrones aims to depict homosexual relationships with complexity and depth, it also risks perpetuating negative stereotypes and sensationalizing trauma for dramatic effect. This dual portrayal underscores the broader challenge within popular media to balance representation with responsible storytelling. The study emphasizes the necessity for more nuanced and empathetic portrayals of marginalized groups to promote greater understanding and inclusivity in media narratives.
Case report: Imaging of septic arthritis in the hip joint of a calf treated with femoral head ostectomy
Authors: Tsuka Takeshi , Okamoto Yoshiharu , Nishiyama Atsushi , Sunden Yuji , Morita Takehito
Published: July 2024

A 24-day-old male Japanese Black calf presented with weight-bearing lameness in the left hind limb and a swollen pelvis. Ultrasonography revealed the accumulation of fluidity materials with a mixture of hyper- and hypo-echogenicity, enclosed by 5–10-mm-thick capsular structures. On the ventral-dorsal radiograph of the pelvis, irregular and radiolucent osseous changes were evident in the femoral head and acetabulum within the swollen hip joint, with soft-tissue density. Computed tomography (CT) confirmed the relationship between the bony and soft tissue lesions, which was suspected following ultrasonography and radiography, and provided additional findings, such as intra-articular accumulation of gas and the degree of osteolytic changes. Based on the imaging findings and cytology of the arthrocentesis specimen, the patient was diagnosed with hip arthritis and osteomyelitis of the femoral head. Additionally, the severity of the lesions supported our decision to perform a femoral head ostectomy. The postoperative radiographic and CT findings of the treated pelvis were helpful in evaluating the efficacy of this technique. Following treatment, the animal could walk and showed normal development, although it was three postoperative months before weight-bearing lameness improved. To the best of our knowledge, this is the first report to describe the combined use of ultrasonography, radiography, and CT in the diagnosis, preoperative planning, and evaluation of the postoperative effects of bovine hip arthritis. Additionally, this report details the therapeutic efficacy of femoral head ostectomy for bovine hip arthritis, a technique that has not been reported previously.
Biologic and genomic characterization of a novel virulent Aeromonas hydrophila phage phiA051, with high homology to prophages
Authors: Wang Yuzhi , Tong Guixiang , Jiang Xinglong , Tu Chuandeng , Cai Hongjiao , Fang Wenhong , Tan Honglian , Weng Qibiao , Wei Xinxian , Lin Mao
Published: July 2024

Introduction

Aeromonas hydrophila is particularly harmful to freshwater aquaculture, and the search for phage is an effective biological control method, but reports of possible temperate phages and their mutants are rare in this field. In this study, a virulent phage highly homologous to prophage in the genomes of A. hydrophila was collected and preliminary biological characterization was carried out to understand its nature.

Materials and methods

Water samples taken from eel ponds in Fujian, China were combined with the strain. Spot test method and double-layer agar plate assay was used for confirmation and purification. Phage virions were observed using transmission electron microscope. A total of 68 strains of Aeromonas spp. were used to determine the host range. MOI groups of 1,000, 100, 10, 1, 0.1, 0.01, 0.001, 0.0001, 0.00001 were prepared to detect the optimal MOI. The conditions of thermal stability assay were set as 30, 40, 50, 60, 70 and 80°C for 1 h, respectively, and conditions of acid and alkali stability assay were set as 2.0, 4.0, 6.0, 8.0, 10.0 and 12.0 of pH. MOI of 0.01 and 0.1, respectively, are set to determine the inhibitory capacity of phage.

Results

A novel virulent A. hydrophila phage designated phiA051 has been isolated from aquaculture water. Electron microscopic observation showed that the phage phiA051 was composed of an icosahedral capsid. The phage phiA051 possesses an optimal multiplicity of infection (MOI) of 0.01, and its burst size was 108 PFU/cell. The phage maintained a high viability at temperatures of 30–50°C or pH 6.0–10.0 for 1  h. Phage phiA051 has certain potentials in rapidly inhibiting the spread of pathogen early in the outbreak, and it has a linear dsDNA with GC content of 60.55% and a total length of 32,212  bp, including 46 ORFs.

Discussion

The phage phiA051 behaved as a virulent phage. However, the BLASTN result showed that 23 of the top 25 hits were genomes of Aeromonas strains. It was suggested that phiA051 was probably derived from some prophage in the chromosome of Aeromonas. Further investigation of the mechanism how phage phiA051 transforms from a temperate phage to a virulent phage will provide a unique perspective and idea to explore the potential of prophages.
Surgical reconstruction of a composite nasomaxillary and superior labial defect in a dog with a fascia lata graft, titanium mesh implant and angularis oris axial pattern flap
Authors: Tu Tsung-Han , Thatcher Graham P. , Soukup Jason W.
Published: July 2024

Objective

To document the successful surgical reconstruction of a composite nasomaxillary and superior labial defect using a fascia lata graft, titanium mesh and angularis oris axial pattern flap in a dog.

Case summary

An estimated 2-year-old female intact mixed-breed dog was presented with a composite (hard and soft tissue) nasomaxillary defect, suspected to be caused by a chemical burn. Physical examination revealed nasal discharge, exposed bilateral maxilla and nasal bone, nasomaxillary fistula with air movement, and intrinsic discoloration of the left maxillary canine tooth. The soft tissue lesion extended from the nasal planum rostrally to the medial canthus of the left eye distally and from the right maxillary bone to include a full thickness loss of the left maxillary labium laterally. Computed tomographic images of the head showed chronic osteomyelitis of the maxilla, zygomatic and nasal bones with nasomaxillary fistula and numerous exposed roots of the left maxillary premolars. Staged surgical procedures to address the dentition and nasomaxillary defect were planned. The first procedure consisted of the extraction of periodontally compromised left maxillary premolars, and standard root canal therapy of bilateral maxillary canine teeth. The second procedure consisted of debridement of the non-vital soft and hard tissues and surgical reconstruction of the nasomaxillary defect after virtual surgical planning. Head computed tomography performed 5 months post-operatively revealed a decrease in the size of the osseous defect as well as the resolution of rhinitis.

Clinical relevance

This case demonstrates the feasibility of using a combination of soft tissue graft, titanium mesh, and axial pattern flap in managing nasomaxillary defects. Such defects can lead to chronic rhinitis, infection, discomfort, and long-term morbidity. This case report provides a novel but practical approach for managing defects in the nasomaxillary region in dogs.
Serological survey on Hepatitis E virus in Namibian dogs, cats, horses, and donkeys
Authors: Molini Umberto , Franzo Giovanni , de Villiers Lourens , van Zyl Leandra , de Villiers Mari , Khaiseb Siegfried , Busch Frank , Knauf Sascha , Dietze Klaas , Eiden Martin
Published: July 2024

The present study investigated the seropositivity rate of Hepatitis E virus (HEV) in domestic and working animals in Namibia, which included dogs, cats, horses, and donkeys. HEV poses a growing threat as a significant cause of human hepatitis globally and has several genotypes of varying zoonotic potential. As epidemiological data on the seroprevalence of HEV in Namibia is scarce, a serosurvey was conducted on archived serum samples of 374 dogs, 238 cats, 98 horses, and 60 donkeys collected between 2018 and 2022 from different regions, to assess the potential of these animals as sources of HEV infection. The findings revealed that 10.43% (n = 39/374) canine and 5.88% (n = 14/238) feline samples tested positive for HEV antibodies, whereas no seropositivity was detected in horses and donkeys. The study further examined the risk factors associated with HEV seropositivity, including animal sex, age, and geographical region, and noted a higher prevalence in dogs living in areas with intensive pig farming. Although there is no direct evidence indicating that these animals served as major reservoirs for HEV transmission to humans, the study underscores the importance of preventive measures to minimize contact exposure with pets considering the potential zoonotic risk, especially for susceptible risk groups. Further research is needed to explore the zoonotic potential of domestic animals and the epidemiological links between animal and human HEV transmissions in Namibia.
GS-441524 and molnupiravir are similarly effective for the treatment of cats with feline infectious peritonitis
Authors: Sase Okihiro , Iwami Tomoko , Sasaki Takeru , Sano Tadashi
Published: July 2024

Although not registered for feline infectious peritonitis (FIP) in Japan, nucleoside analogs have shown efficacy and we have been offering them to owners of cats with FIP at our clinic since January 2020. The aim of this study was to investigate outcomes in cats with FIP who received GS-441524 or molnupiravir. Diagnosis of FIP was based on clinical signs, laboratory test results, and the presence of feline coronavirus RNA in blood or effusion aspirate. After providing verbal and written information, owners of cats with a presumptive diagnosis of FIP with a were offered antiviral treatment with commercially sourced GS-441524 from June 2020, and either GS-441524 or compounded molnupiravir from January 2022. Dosing was 12.5–25 mg/kg/day for GS-441524 and 20–40 mg/kg/day for molnupiravir, depending on the presence of effusion and neurological and/or ocular signs, and continued for 84 days. Overall, 118 cats with FIP (effusive in 76) received treatment, 59 with GS-4421524 and 59 with molnupiravir. Twenty cats died, 12/59 (20.3%) in the GS-441524 group and 8/59 (13.6%) in the molnupiravir group (p = 0.326), with most deaths within the first 10 days of starting treatment. Among survivors, neurological and ocular signs resolved in all but one cat, who had persistent seizures. Of the cats completing treatment, 48/48 in the GS-441524 group and 51/52 in the molnupiravir group achieved remission. Laboratory parameters normalized within 6 to 7 weeks of starting drug administration. Adverse events, such as primarily hepatic function abnormalities, were transient and resolved without specific intervention. Our data indicate that GS-441524 and molnupiravir show similar effects and safety in cats with FIP.
Influence of Rotor Cage Structural Parameters on the Classification Performance of a Straw Micro-Crusher Classifying Device: CFD and Machine Learning Approach
Authors: Fu Min , Cao Zhong , Zhan Mingyu , Wang Yulong , Chen Lei
Published: July 2024

The rotor cage is a key component of the classifying device, and its structural parameters directly affect classification performance. To improve the classification performance of the straw micro-crusher classifying device, this paper proposes a CFD-ML-GA (Computational Fluid Dynamics-Machine Learning-Genetic Algorithm) method to quantitatively analyze the coupled effects of rotor cage structural parameters on classification performance. Firstly, CFD and orthogonal experimental methods are used to qualitatively investigate the effects of the number of blades, length of rotor blades, and blade installation angle on the classification performance. The conclusion obtained is that the blade installation angle exerts the greatest effect on classification performance, while the number of blades has the least effect. Subsequently, four machine learning algorithms are used to build a cut size prediction model, and, after comparison, the Random Forest Regression (RFR) model is selected. Finally, RFR is integrated with a Genetic Algorithm (GA) for quantitative parameter optimization. The quantitative analysis results of GA indicate that with 29 blades, a blade length of 232.8 mm, and a blade installation angle of 36.8°, the cut size decreases to 47.6 μm and the classifying sharpness index improves to 0.62. Compared with the optimal solution from the orthogonal experiment, the GA solution reduces the cut size by 9.33% and improves the classifying sharpness index by 9.68%. This validates the feasibility of the proposed method.
Remote Monitoring of Bee Apiaries as a Tool for Crisis Management
Authors: Rodias Efthymios , Kilimpas Vasileios
Published: July 2024

Apiculture has presented significant growth in the last decades in Europe and worldwide. According to the Food and Agriculture Organization (FAO), there were 25.1 million bee colonies in Europe in 2021, with most of them being located in the southeastern countries. Smart technologies have invaded almost every pillar of agriculture, including apiculture. Modern apiculture is rather more nomadic than sedentary. Nomadism in beekeeping requires monitoring the settlement of bee colonies, in more than one place per year, in order to select more honey and pollen and contribute to the overall growth of the bees. To this scope, it is efficient to monitor and have wide control of bees remotely, in parallel with other smart applications, in order to prevent crises that would affect bee survival and/or yield production. The objectives of this paper are to outline a series of automation systems in apiculture used as a means towards the optimization of bee apiary management processes. Four beekeepers’ case studies were used to demonstrate how sensors and communication means transfer multiple bee-related data from various bee apiary locations to a single control system. The methodology was based on input/output data evaluation, risk prioritization based on real data, and feedback to the beekeeper based on the potential risks. Based on the results, the most significant risks are related to bad weather conditions, varroa mites, and bee colony health. Furthermore, the beekeeper is able to optimize the whole management, operations, and strategic planning throughout the year. Last, it should be noted that the presented remote monitoring system will never substitute the necessity of traditional beekeeper visits, but it contributes to minimizing them based on the monitored daily data.