bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Pressurizing Cryogenic Treatments on Physical and Mechanical Properties of Shale Core Samples—An Experimental Study
Authors: Khalil Rayan , Emadi Hossein , Altawati Faisal
Published: January 2021

The technique of cryogenic treatments requires injecting extremely cold fluids such as liquid nitrogen (LN2) into formations to create fractures in addition to connecting pre-existing fracture networks. This study investigated the effects of implementing and pressurizing cryogenic treatment on the physical (porosity and permeability) and mechanical properties (Young’s modulus, Poisson’s ratio, and bulk compressibility) of the Marcellus shale samples. Ten Marcellus core samples were inserted in a core holder and heated to 66 °C using an oven. Then, LN2 (−177 °C) was injected into the samples at approximately 0.14 MPa. Nitrogen was used to pressurize nine samples at injection pressures of 1.38, 2.76, and 4.14 MPa while the tenth core sample was not pressurized. Using a cryogenic pressure transducer and a T-type thermocouple, the pressure and temperature of the core holder were monitored and recorded during the test. The core samples were scanned using a computed tomography (CT) scanner, and their porosities, permeability, and ultrasonic velocities were measured both before and after conducting the cryogenic treatments. The analyses of CT scan results illustrated that conducting cryogenic treatments created new cracks inside all the samples. These cracks increased the pore volume, and as a result, the porosity, permeability, and bulk compressibility of the core samples increased. The creations of the new cracks also resulted in reductions in the compressional and shear velocities of the samples, and as a result, decreasing the Young’s modulus and Poisson’s ratio. Moreover, the results revealed that pressurizing the injected LN2 increased the alterations of aforementioned properties.
Evolution of Malaria Incidence in Five Health Districts, in the Context of the Scaling up of Seasonal Malaria Chemoprevention, 2016 to 2018, in Mali
Authors: Sacko Aissata , Sagara Issaka , Berthé Ibrahima , Diarra Modibo , Cissoko Mady , Diarra Souleymane S. , Coulibaly Drissa , Sanogo Moussa , Dicko Alassane
Published: January 2021

Context: In Mali, malaria transmission is seasonal, exposing children to high morbidity and mortality. A preventative strategy called Seasonal Malaria Chemoprevention (SMC) is being implemented, consisting of the distribution of drugs at monthly intervals for up to 4 months to children between 3 and 59 months of age during the period of the year when malaria is most prevalent. This study aimed to analyze the evolution of the incidence of malaria in the general population of the health districts of Kati, Kadiolo, Sikasso, Yorosso, and Tominian in the context of SMC implementation. Methods: This is a transversal study analyzing the routine malaria data and meteorological data of Nasa Giovanni from 2016 to 2018. General Additive Model (GAM) analysis was performed to investigate the relationship between malaria incidence and meteorological factors. Results: From 2016 to 2018, the evolution of the overall incidence in all the study districts was positively associated with the relative humidity, rainfall, and minimum temperature components. The average monthly incidence and the relative humidity varied according to the health district, and the average temperature and rainfall were similar. A decrease in incidence was observed in children under five years old in 2017 and 2018 compared to 2016. Conclusion: A decrease in the incidence of malaria was observed after the SMC rounds. SMC should be applied at optimal periods.
Medical Students and COVID-19: Knowledge, Preventive Behaviors, and Risk Perception
Authors: Alsoghair Mansour , Almazyad Mohammad , Alburaykan Tariq , Alsultan Abdulrhman , Alnughaymishi Abdulmajeed , Almazyad Sulaiman , Alharbi Meshari , Alkassas Wesam , Almadud Abdulaziz , Alsuhaibani Mohammed
Published: January 2021

Background: The coronavirus disease (COVID-19) pandemic is an international public health threat. This study aimed to evaluate COVID-19-related knowledge, preventive behaviors, and risk perception among Saudi Arabian medical students and interns. Materials and Methods: This cross-sectional study was conducted among fourth- and fifth-year medical students and interns between June and August 2020 at three colleges of medicine in Qassim Region, Saudi Arabia. A previously validated questionnaire was distributed as an online survey. Results: The total mean knowledge score was 12.5/15 points; 83.9% achieved a high score. The mean score of self-reported preventive behavior was 8.40; 94.1% achieved a high score. The overall mean risk perception score was 5.34/8 points; 31.6% achieved a high score. Conclusion: Medical students assessed in this study displayed sufficient knowledge and preventive behaviors regarding the COVID-19 pandemic and an average level of risk perception. Lower scores by younger medical students suggest that they must improve their COVID-19 knowledge and risk perception, as they are a potential source of health information in their communities.
Examining Cervical Cancer Preventive Behaviors for Latinx Transmasculine Individuals among Medical Students
Authors: Ramos-Pibernus Alíxida , Carminelli-Corretjer Paola , Bermonti-Pérez Mario , Tollinchi-Natali Nelmit , Jiménez-Ricaurte Coral , Mejías-Serrano David , Silva-Reteguis Julian , Moreta-Ávila Fabian , Blanco Malynie , Justiz Lellanes , Febo Marta , Rivera-Segarra Eliut
Published: January 2021

Latinx transmasculine men (LTM) can be at a particularly high risk for cervical cancer as they lie at the intersection of two health disparity populations (gender and ethnic minorities). Previous research using self-report measures has documented how negative interactions with providers are a key barrier for cervical cancer screening among LTM. However, no research to date has examined, via direct observation, cervical cancer preventive behaviors in clinical interactions with LTM. Thus, the objective of this study was to examine cervical cancer preventive behaviors in clinical interactions between medical students and an LTM. The team implemented standardized patient simulations (simulations of clinical interactions with actors portraying the role of a patient), self-report measures, and observational techniques. A total of 37 medical students participated in the study. The results were mixed with some key behaviors neglected (i.e., asking if the patient preferred to collect the HPV test sample by himself), while others were enacted (i.e., checking family history of cervical cancer). Further research is needed to better understand behaviors in clinical interactions with LTM as well as how to improve them.
Facilitating Sustainable Disaster Risk Reduction in Indigenous Communities: Reviving Indigenous Worldviews, Knowledge and Practices through Two-Way Partnering
Authors: Ali Tahir , Buergelt Petra Topaz , Paton Douglas , Smith James Arnold , Maypilama Elaine Lawurrpa , Yuŋgirrŋa Dorothy , Dhamarrandji Stephen , Gundjarranbuy Rosemary
Published: January 2021

The Sendai Framework of Action 2015–2030 calls for holistic Indigenous disaster risk reduction (DRR) research. Responding to this call, we synergized a holistic philosophical framework (comprising ecological systems theory, symbolic interactionism, and intersectionality) and social constructionist grounded theory and ethnography within a critical Indigenous research paradigm as a methodology for exploring how diverse individual and contextual factors influence DRR in a remote Indigenous community called Galiwinku, in the Northern Territory of Australia. Working together, Indigenous and non-Indigenous researchers collected stories in local languages using conversations and yarning circles with 20 community members, as well as participant observations. The stories were interpreted and analysed using social constructivist grounded theory analysis techniques. The findings were dialogued with over 50 community members. The findings deeply resonated with the community members, validating the trustworthiness and relevance of the findings. The grounded theory that emerged identified two themes. First, local Indigenous knowledge and practices strengthen Indigenous people and reduce the risks posed by natural hazards. More specifically, deep reciprocal relationships with country and ecological knowledge, strong kinship relations, Elder’s wisdom and authority, women and men sharing power, and faith in a supreme power/God and Indigenous-led community organizations enable DRR. Second, colonizing practices weaken Indigenous people and increase the risks from natural hazards. Therefore, colonization, the imposition of Western culture, the government application of top-down approaches, infiltration in Indigenous governance systems, the use of fly-in/fly-out workers, scarcity of employment, restrictions on technical and higher education opportunities, and overcrowded housing that is culturally and climatically unsuitable undermine the DRR capability. Based on the findings, we propose a Community-Based DRR theory which proposes that facilitating sustainable Indigenous DRR in Australian Indigenous communities requires Indigenous and non-Indigenous partners to genuinely work together in two-directional and complementary ways.
Menstrual Cycle Phases Influence on Cardiorespiratory Response to Exercise in Endurance-Trained Females
Authors: Rael Beatriz , Alfaro-Magallanes Víctor , Romero-Parra Nuria , Castro Eliane , Cupeiro Rocío , Janse de Jonge Xanne , Wehrwein Erica , Peinado Ana ,   IronFEMME Study Group
Published: January 2021

The aim of this study was to analyse the impact of sex hormone fluctuations throughout the menstrual cycle on cardiorespiratory response to high-intensity interval exercise in athletes. Twenty-one eumenorrheic endurance-trained females performed an interval running protocol in three menstrual cycle phases: early-follicular phase (EFP), late-follicular phase (LFP) and mid-luteal phase (MLP). It consisted of 8 × 3-min bouts at 85% of their maximal aerobic speed with 90-s recovery at 30% of their maximal aerobic speed. To verify menstrual cycle phase, we applied a three-step method: calendar-based counting, urinary luteinizing hormone measurement and serum hormone analysis. Mixed-linear model for repeated measures showed menstrual cycle impact on ventilatory (EFP: 78.61 ± 11.09; LFP: 76.45 ± 11.37; MLP: 78.59 ± 13.43) and heart rate (EFP: 167.29 ± 11.44; LFP: 169.89 ± 10.62; MLP: 169.89 ± 11.35) response to high-intensity interval exercise (F2.59 = 4.300; p = 0.018 and F2.61 = 4.648; p = 0.013, respectively). Oxygen consumption, carbon dioxide production, respiratory exchange ratio, breathing frequency, energy expenditure, relative perceived exertion and perceived readiness were unaltered by menstrual cycle phase. Most of the cardiorespiratory variables measured appear to be impassive by menstrual cycle phases throughout a high-intensity interval exercise in endurance-trained athletes. It seems that sex hormone fluctuations throughout the menstrual cycle are not high enough to disrupt tissues’ adjustments caused by the high-intensity exercise. Nevertheless, HR based training programs should consider menstrual cycle phase.
Indoor Cycling Energy Expenditure: Does Sequence Matter?
Authors: Cortis Cristina , Fusco Andrea , Cook Mitchell , Doberstein Scott T. , Gillette Cordial , Porcari John P. , Foster Carl
Published: January 2021

Although cycling class intensity can be modified by changing interval intensity sequencing, it has not been established whether the intensity order can alter physiological and perceptual responses. Therefore, this study aimed to determine the effects of interval intensity sequencing on energy expenditure (EE), physiological markers, and perceptual responses during indoor cycling. Healthy volunteers (10 males = 20.0 ± 0.8years; 8 females = 21.3 ± 2.7years) completed three randomly ordered interval bouts (mixed pyramid—MP, ascending intervals—AI, descending intervals—DI) including three 3-min work bouts at 50%, 75%, and 100% of peak power output (PPO) and three 3-min recovery periods at 25% PPO. Heart rate (HR) and oxygen consumption (VO2) were expressed as percentages of maximal HR (%HRmax) and VO2 (%VO2max). EE was computed for both the work bout and for the 5-min recovery period. Session Rating of Perceived Exertion (sRPE) and Exercise Enjoyment Scale (EES) were recorded. No differences emerged for % HRmax (MP = 73.3 ± 6.1%; AI = 72.1 ± 4.9%; DI = 71.8 ± 4.5%), % VO2max (MP = 51.8 ± 4.6%; AI = 51.4 ± 3.9%; DI = 51.3 ± 4.5%), EE (MP = 277.5 ± 39.9 kcal; AI = 275.8 ± 39.4 kcal; DI = 274.9 ± 42.1 kcal), EES (MP = 4.9 ± 1.0; AI = 5.3 ± 1.1; DI = 4.9 ± 0.9), and sRPE (MP = 4.9 ± 1.0; AI = 5.3 ± 1.1; DI = 4.9 ± 0.9). EE during recovery was significantly (p < 0.005) lower after DI (11.9 ± 3.2 kcal) with respect to MP (13.2 ± 2.5 kcal) and AI (13.3 ± 2.5 kcal). Although lower EE was observed during recovery in DI, interval intensity sequencing does not affect overall EE, physiological markers, and perceptual responses.
Spatial–Temporal Distribution Variation of Ground-Level Ozone in China’s Pearl River Delta Metropolitan Region
Authors: Zhang An , Lin Jinhuang , Chen Wenhui , Lin Mingshui , Lei Chengcheng
Published: January 2021

Long-term exposure to ozone pollution will cause severe threats to residents’ physical and mental health. Ground-level ozone is the most severe air pollutant in China’s Pearl River Delta Metropolitan Region (PRD). It is of great significance to accurately reveal the spatial–temporal distribution characteristics of ozone pollution exposure patterns. We used the daily maximum 8-h ozone concentration data from PRD’s 55 air quality monitoring stations in 2015 as input data. We used six models of STK and ordinary kriging (OK) for the simulation of ozone concentration. Then we chose a better ozone pollution prediction model to reveal the ozone exposure characteristics of the PRD in 2015. The results show that the Bilonick model (BM) model had the highest simulation precision for ozone in the six models for spatial–temporal kriging (STK) interpolation, and the STK model’s simulation prediction results are significantly better than the OK model. The annual average ozone concentrations in the PRD during 2015 showed a high spatial variation in the north and east and low in the south and west. Ozone concentrations were relatively high in summer and autumn and low in winter and spring. The center of gravity of ozone concentrations tended to migrate to the north and west before moving to the south and then finally migrating to the east. The ozone’s spatial autocorrelation was significant and showed a significant positive correlation, mainly showing high-high clustering and low-low clustering. The type of clustering undergoes temporal migration and conversion over the four seasons, with spatial autocorrelation during winter the most significant.
Mediational Occupational Risk Factors Pertaining to Work Ability According to Age, Gender and Professional Job Type
Authors: Mateo-Rodríguez Inmaculada , Knox Emily , Oliver-Hernández Coral , Daponte-Codina Antonio ,   on behalf of the esTAR Group
Published: January 2021

The predictive value of work ability for several health and occupational outcomes is well known. Maintaining the ability to work of all employees has become an important topic in research although some evidence suggests that some groups of workers need greater attention than others. Healthcare workers (x¯ = 54.46 ± 5.64 years) attending routine occupational health checkups completed their work ability, occupational risk and sociodemographic measures. An analysis examined whether work ability differed according to gender, age and professional category. Mediation of these relationships by occupational risk variables, such as work–family conflict, was examined. Females and older adults had worse work ability than their counterparts. Professional group was not independently associated. Gender-related differences were mediated by current and historic ergonomic risk, psychosocial risk and work–family conflict. Age-related differences were mediated by violence/discrimination at work. All job risk variables, apart from current ergonomic risk, mediated associations between professional category and work ability. The present study identified the importance of risk variables for the work ability of health workers according to gender, age and professional job type. Perceptions of work–family conflict and violence–discrimination seem particularly important and should be considered when targeting improvements in work ability.
A Short Form of the Chinese Version of the Weinstein Noise Sensitivity Scale through Optimal Test Assembly
Authors: Li Sha , Fong Daniel Yee Tak , Wan Sarah Lai Yin , McPherson Bradley , Lau Esther Yuet Ying , Huang Lixi , Ip Mary Sau Man , Wong Janet Yuen Ha
Published: January 2021

This study developed a short form of the traditional Chinese version of the Weinstein Noise Sensitivity Scale (WNSS) through optimal test assembly (OTA). A total of 1069 Chinese adults (64.8% female) completed the territory-wide cross-sectional study. We first removed Items 12 and 5 which had negative factor loading and gender-related differential item functioning (DIF), respectively. The optimal length was then determined as the minimal one that reasonably resembled the reliability and validity of the scale without DIF items. OTA identified an 8-item WNSS (WNSS-8) which retained 67.2% of the test information of the original 21-item scale and had a Cronbach’s alpha of 0.83. It also showed significant correlations of 0.272 and −0.115 with the neuroticism and extraversion scales of Chinese NEO-Five Factor Inventory, respectively. Adequate model fit of the WNSS-8 was demonstrated by the confirmatory factor analysis. The Chinese WNSS-8 can be used to assess noise sensitivity without compromising reliability and validity.
Relationship between Training Factors and Injuries in Stand-Up Paddleboarding Athletes
Authors: Castañeda-Babarro Arkaitz , Calleja-González Julio , Viribay Aitor , Fernández-Lázaro Diego , León-Guereño Patxi , Mielgo-Ayuso Juan
Published: January 2021

Stand-up paddleboarding (SUP) is an increasingly popular sport but, as in other sports, there is an injury ratio associated with practicing it. In other types of sport, some factors have been linked to the likelihood of suffering an injury, among which stretching, core training and resistance training may be considered the most significant. Therefore, the main aim of this study was to identify the training factors that could influence injuries suffered by participants in international SUP competitions. Ninety-seven questionnaires were collected from paddlers who participated in an international SUP circuit, with epidemiological data being gathered about injuries and different questions related to the training undertaken. A multi-factor ANOVA test was used to identify the factors which influence the state of injury. Results showed that almost 60% of injuries occurred in the arms or in the upper thoracic region, around 65% of which were in tendons or muscles and, in almost half of cases, were related to overuse. Likewise, the results showed that athletes with injury performed fewer resistance training sessions per week (p = 0.028), over fewer months per year (p = 0.001), more weekly training sessions (p = 0.004) and, lastly, a greater volume of weekly training (p = 0.003) than athletes without injury. Moreover, the most important training factors that reduce the likelihood of suffering an injury were taken into account-in. particular, resistance training alone (p = 0.011) or together with CORE training (p = 0.006) or stretching (p = 0.012), and the dominant side of paddling (p = 0.032). In conclusion, resistance training would seem to reduce the likelihood of injury among SUP practitioners, and such benefits could be obtained by resistance training alone or in combination with CORE training or stretching.
Flow Biocatalysis: A Challenging Alternative for the Synthesis of APIs and Natural Compounds
Authors: Santi Micol , Sancineto Luca , Nascimento Vanessa , Braun Azeredo Juliano , Orozco Erika V. M. , Andrade Leandro H. , Gröger Harald , Santi Claudio
Published: January 2021

Biocatalysts represent an efficient, highly selective and greener alternative to metal catalysts in both industry and academia. In the last two decades, the interest in biocatalytic transformations has increased due to an urgent need for more sustainable industrial processes that comply with the principles of green chemistry. Thanks to the recent advances in biotechnologies, protein engineering and the Nobel prize awarded concept of direct enzymatic evolution, the synthetic enzymatic toolbox has expanded significantly. In particular, the implementation of biocatalysts in continuous flow systems has attracted much attention, especially from industry. The advantages of flow chemistry enable biosynthesis to overcome well-known limitations of “classic” enzymatic catalysis, such as time-consuming work-ups and enzyme inhibition, as well as difficult scale-up and process intensifications. Moreover, continuous flow biocatalysis provides access to practical, economical and more sustainable synthetic pathways, an important aspect for the future of pharmaceutical companies if they want to compete in the market while complying with European Medicines Agency (EMA), Food and Drug Administration (FDA) and green chemistry requirements. This review focuses on the most recent advances in the use of flow biocatalysis for the synthesis of active pharmaceutical ingredients (APIs), pharmaceuticals and natural products, and the advantages and limitations are discussed.
Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats
Authors: Mrozkova Petra , Spicarova Diana , Palecek Jiri
Published: January 2021

The mechanisms of inflammatory pain need to be identified in order to find new superior treatments. Protease-activated receptors 2 (PAR2) and transient receptor potential vanilloid 1 (TRPV1) are highly co-expressed in dorsal root ganglion neurons and implicated in pain development. Here, we examined the role of spinal PAR2 in hyperalgesia and the modulation of synaptic transmission in carrageenan-induced peripheral inflammation, using intrathecal (i.t.) treatment in the behavioral experiments and recordings of spontaneous, miniature and dorsal root stimulation-evoked excitatory postsynaptic currents (sEPSCs, mEPSCs and eEPSCs) in spinal cord slices. Intrathecal PAR2-activating peptide (AP) administration aggravated the carrageenan-induced thermal hyperalgesia, and this was prevented by a TRPV1 antagonist (SB 366791) and staurosporine i.t. pretreatment. Additionally, the frequency of the mEPSC and sEPSC and the amplitude of the eEPSC recorded from the superficial dorsal horn neurons were enhanced after acute PAR2 AP application, while prevented with SB 366791 or staurosporine pretreatment. PAR2 antagonist application reduced the thermal hyperalgesia and decreased the frequency of mEPSC and sEPSC and the amplitude of eEPSC. Our findings highlight the contribution of spinal PAR2 activation to carrageenan-induced hyperalgesia and the importance of dorsal horn PAR2 and TRPV1 receptor interactions in the modulation of nociceptive synaptic transmission.
Clinical Validation of an Automated Fluorogenic Factor XIII Activity Assay Based on Isopeptidase Activity
Authors: Leitner Martina , Büchold Christian , Pasternack Ralf , Binder Nikolaus B. , Moore Gary W.
Published: January 2021

Hereditary factor XIII (FXIII) deficiency is a rare autosomal bleeding disorder which can cause life-threatening bleeding. Acquired deficiency can be immune-mediated or due to increased consumption or reduced synthesis. The most commonly used screening test is insensitive, and widely used quantitative assays have analytical limitations. The present study sought to validate Technofluor FXIII Activity, the first isopeptidase-based assay available on a routine coagulation analyser, the Ceveron s100. Linearity was evidenced throughout the measuring range, with correlation coefficients of >0.99, and coefficients of variation for repeatability and reproducibility were <5% and <10%, respectively. A normally distributed reference range of 47.0–135.5 IU/dL was derived from 154 normal donors. Clinical samples with Technofluor FXIII Activity results between 0 and 167.0 IU/dL were assayed with Berichrom® FXIII Activity, a functional ammonia release assay, and the HemosIL™ FXIII antigen assay, generating correlations of 0.950 and 0.980, respectively. Experiments with a transglutaminase inhibitor showed that Technofluor FXIII Activity can detect inhibition of enzymatic activity. No interference was exhibited by high levels of haemolysis and lipaemia, and interference by bilirubin was evident at 18 mg/dL, a level commensurate with severe liver disease. Technofluor FXIII Activity is a rapid, accurate and precise assay suitable for routine diagnostic use with fewer interferents than ammonia release FXIII activity assays.
Detection of Bacterial Membrane Vesicles by NOD-Like Receptors
Authors: Johnston Ella L. , Heras Begoña , Kufer Thomas A. , Kaparakis-Liaskos Maria
Published: January 2021

Bacterial membrane vesicles (BMVs) are nanoparticles produced by both Gram-negative and Gram-positive bacteria that can function to modulate immunity in the host. Both outer membrane vesicles (OMVs) and membrane vesicles (MVs), which are released by Gram-negative and Gram-positive bacteria, respectively, contain cargo derived from their parent bacterium, including immune stimulating molecules such as proteins, lipids and nucleic acids. Of these, peptidoglycan (PG) and lipopolysaccharide (LPS) are able to activate host innate immune pattern recognition receptors (PRRs), known as NOD-like receptors (NLRs), such as nucleotide-binding oligomerisation domain-containing protein (NOD) 1, NOD2 and NLRP3. NLR activation is a key driver of inflammation in the host, and BMVs derived from both pathogenic and commensal bacteria have been shown to package PG and LPS in order to modulate the host immune response using NLR-dependent mechanisms. Here, we discuss the packaging of immunostimulatory cargo within OMVs and MVs, their detection by NLRs and the cytokines produced by host cells in response to their detection. Additionally, commensal derived BMVs are thought to shape immunity and contribute to homeostasis in the gut, therefore we also highlight the interactions of commensal derived BMVs with NLRs and their roles in limiting inflammatory diseases.
Identification of Circulating Serum miRNAs as Novel Biomarkers in Pancreatic Cancer Using a Penalized Algorithm
Authors: Lee Jaehoon , Lee Hee Seung , Park Soo Been , Kim Chanyang , Kim Kahee , Jung Dawoon E. , Song Si Young
Published: January 2021

Pancreatic cancer (PC) is difficult to detect in the early stages; thus, identifying specific and sensitive biomarkers for PC diagnosis is crucial, especially in the case of early-stage tumors. Circulating microRNAs are promising non-invasive biomarkers. Therefore, we aimed to identify non-invasive miRNA biomarkers and build a model for PC diagnosis. For the training model, blood serum samples from 63 PC patients and 63 control subjects were used. We selected 39 miRNA markers using a smoothly clipped absolute deviation-based penalized support vector machine and built a PC diagnosis model. From the double cross-validation, the average test AUC was 0.98. We validated the diagnosis model using independent samples from 25 PC patients and 81 patients with intrahepatic cholangiocarcinoma (ICC) and compared the results with those obtained from the diagnosis using carbohydrate antigen 19-9. For the markers miR-155-5p, miR-4284, miR-346, miR-7145-5p, miR-5100, miR-661, miR-22-3p, miR-4486, let-7b-5p, and miR-4703-5p, we conducted quantitative reverse transcription PCR using samples from 17 independent PC patients, 8 ICC patients, and 8 healthy individuals. Differential expression was observed in samples from PC patients. The diagnosis model based on the identified markers showed high sensitivity and specificity for PC detection and is potentially useful for early PC diagnosis.
A Supramolecular Assembly of Hemoproteins Formed in a Star-Shaped Structure via Heme–Heme Pocket Interactions
Authors: Soon Julian Wong , Oohora Koji , Hirayama Shota , Hayashi Takashi
Published: January 2021

Proteins have been used as building blocks to provide various supramolecular structures in efforts to develop nano-biomaterials possessing broad biological functionalities. A series of unique structures have been obtained from the engineering of hemoproteins which contain the iron porphyrin known as heme, as a prosthetic group. This work in developing assembling systems is extended using cytochrome b562, a small electron transfer hemoprotein engineered to include an externally-attached heme moiety. The engineered units, which form a one-dimensional assembly via interprotein heme–heme pocket interactions, are conjugated to an apo-form of hexameric tyrosine-coordinated hemoprotein (apoHTHP) to provide a branching unit promoting the assembly of a star-shaped structure. The incorporation of the heme moiety attached to the protein surface of cytochrome b562 into apoHTHP can be accelerated by elevating the reaction temperature to generate a new assembly. The formation of a new larger assembly structure was confirmed by size exclusion chromatography. The ratio of the heme-containing units in the assemblies was analyzed by UV-Vis spectroscopy and the population of protein units estimated from SDS PAGE suggests the presence of plausible star-shaped structures, which are supported by hydrodynamic diameter data obtained by dynamic light scattering.
The Lactococcal dgkB (yecE) and dxsA Genes for Lipid Metabolism Are Involved in the Resistance to Cell Envelope-Acting Antimicrobials
Authors: Tymoszewska Aleksandra , Aleksandrzak-Piekarczyk Tamara
Published: January 2021

The emergence of antibiotic-resistant bacteria led to an urgent need for next-generation antimicrobial agents with novel mechanisms of action. The use of positively charged antimicrobial peptides that target cytoplasmic membrane is an especially promising strategy since essential functions and the conserved structure of the membrane hinder the development of bacterial resistance. Aureocin A53- and enterocin L50-like bacteriocins are highly cationic, membrane-targeting antimicrobial peptides that have potential as next-generation antibiotics. However, the mechanisms of resistance to these bacteriocins and cross-resistance against antibiotics must be examined before application to ensure their safe use. Here, in the model bacterium Lactococcus lactis, we studied the development of resistance to selected aureocin A53- and enterocin L50-like bacteriocins and its correlation with antibiotics. First, to generate spontaneous resistant mutants, L.lactis was exposed to bacteriocin BHT-B. Sequencing of their genomes revealed single nucleotide polymorphisms (SNPs) in the dgkB (yecE) and dxsA genes encoding diacylglycerol kinase and 1-deoxy-D-xylulose 5-phosphate synthase, respectively. Then, selected mutants underwent susceptibility tests with a wide array of bacteriocins and antibiotics. The highest alterations in the sensitivity of studied mutants were seen in the presence of cytoplasmic membrane targeting bacteriocins (K411, Ent7, EntL50, WelM, SalC, nisin) and antibiotics (daptomycin and gramicidin) as well as lipid II cycle-blocking bacteriocins (nisin and Lcn972) and antibiotics (bacitracin). Interestingly, decreased via the SNPs accumulation sensitivity to membrane-active bacteriocins and antibiotics resulted in the concurrently increased vulnerability to bacitracin, carbenicillin, or chlortetracycline. It is suspected that SNPs may result in alterations to the efficiency of the nascent enzymes rather than a total loss of their function as neither deletion nor overexpression of dxsA restored the phenotype observed in spontaneous mutants.
Predicting the Effects of Climate Change on Water Temperatures of Roode Elsberg Dam Using Nonparametric Machine Learning Models
Authors: Tshireletso Thalosang , Moyo Pilate , Kabani Matongo
Published: January 2021

A nonparametric machine learning model was used to study the behaviour of the variables of a concrete arch dam: Roode Elsberg dam. The variables used were ambient temperature, water temperatures, and water level. Water temperature was measured using twelve thermometers; six thermometers were on each flank of the dam. The thermometers were placed in pairs on different levels: avg6 (avg6-R and avg6-L) and avg5 (avg5-R and avg5-L) were on level 47.43 m, avg4 (avg4-R and avg4-L) and avg3 (avg3-R and avg3-L) were on level 43.62 m, and avg2 (avg2-R and avg2-L) and avg1 (avg1-R and avg1-L) were on level 26.23 m. Four neural networks and four random forests were cross-validated to determine their best-performing hyperparameters with the water temperature data. Quantile random forest was the best performer at mtry 7 (Number of variables randomly sampled as candidates at each split) and RMSE (Root mean square error) of 0.0015, therefore it was used for making predictions. The predictions were made using two cases of water level: recorded water level and full dam steady-state at Representative Concentration Pathway (RCP) 4.5 (hot and cold model) and RCP 8.5 (hot and cold model). Ambient temperature increased on average by 1.6 °C for the period 2012–2053 when using recorded water level; this led to increases in water temperature of 0.9 °C, 0.8 °C, and 0.4 °C for avg6-R, avg3-R, and avg1-R, respectively, for the period 2012–2053. The same average temperature increase led to average increases of 0.7 °C for avg6-R, 0.6 °C for avg3-R, and 0.3 °C for avg1-R for a full dam steady-state for the period 2012–2053.
Transient Responses Evaluation of FPSO with Different Failure Scenarios of Mooring Lines
Authors: Qiao Dongsheng , Li Binbin , Yan Jun , Qin Yu , Liang Haizhi , Ning Dezhi
Published: January 2021

During the long-term service condition, the mooring line of the deep-water floating platform may fail due to various reasons, such as overloading caused by an accidental condition or performance deterioration. Therefore, the safety performance under the transient responses process should be evaluated in advance, during the design phase. A series of time-domain numerical simulations for evaluating the performance changes of a Floating Production Storage and Offloading (FPSO) with different broken modes of mooring lines was carried out. The broken conditions include the single mooring line or two mooring lines failure under ipsilateral, opposite, and adjacent sides. The resulting transient and following steady-state responses of the vessel and the mooring line tensions were analyzed, and the corresponding influence mechanism was investigated. The accidental failure of a single or two mooring lines changes the watch circle of the vessel and the tension redistribution of the remaining mooring lines. The results indicated that the failure of mooring lines mainly influences the responses of sway, surge, and yaw, and the change rule is closely related to the stiffness and symmetry of the mooring system. The simulation results could give a profound understanding of the transient-effects influence process of mooring line failure, and the suggestions are given to account for the transient effects in the design of the mooring system.
Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)
Authors: Zeng Xinhua , Ni Ziyi , Diao Haixin , Jiang Kai , Hu Chao , Shao Li , Huang Weichang
Published: January 2021

Orchids of the genus Bletilla are well-known ornamental plants and sources of traditional medicine in Asia that rely on the symbiotic relationship with root endophytic fungi throughout their whole life cycle. However, little is known about their fungal partners, infection pattern, and pathways of carbon gain. We investigated carbon and nitrogen stable isotope patterns in different organs of three Bletilla species, identified the root endophytic fungal community composition, and determined mycorrhizal colonization rates. The three Bletilla species were comprised by a polyphyletic group which belongs to different trophic modes, such as saprotroph, pathotroph, and symbiotroph; however, the dominant species and their abundances varied among Bletilla spp. Mycorrhizal infection rates also varied among Bletilla species, with B. striata (65% ± 25%) being significantly higher than those of B. formosana (35% ± 16%) and B. ochracea (22% ± 13%). Compared with surrounding autotrophic plants, all Bletilla spp. were significantly enriched in 13C with B. striata to a significantly higher level than other two Bletilla species. Among different organs, stems had higher δ13C values, while leaves and flowers had higher δ15N and total N content values across all three species. Our results indicate that the symbiotic relationship of Bletilla and its root endophytic fungi is not strictly specific. Although mycorrhizal infection rates were highly variable, the three Bletilla species had the same infection pattern with hyphae penetrating the cortex cell by the pathway cell. Different Bletilla species have different strategies for C allocation among plant organs. These findings provide new insights into the ecological adaptation of orchids and will contribute to Bletilla germplasm conservation and sustainable utilization.
Dexamethasone Promotes Aspergillus fumigatus Growth in Macrophages by Triggering M2 Repolarization via Targeting PKM2
Authors: Luvanda Maureen K. , Posch Wilfried , Vosper Jonathan , Zaderer Viktoria , Noureen Asma , Lass-Flörl Cornelia , Wilflingseder Doris
Published: January 2021

Since long-term corticosteroid treatment is associated with emerging opportunistic fungal infections causing high morbidity and mortality in immune-suppressed individuals, here we characterized the impact of dexamethasone (Dex) treatment on Aspergillus fumigatus-related immune modulation. We found by high content screening and flow cytometric analyses that during monocyte-to-macrophage differentiation, as little as 0.1 µg/mL Dex resulted in a shift in macrophage polarization from M1 to M2-like macrophages. This macrophage repolarization mediated via Dex was characterized by significant upregulation of the M2 marker CD163 and downmodulation of M1 markers CD40 and CD86 as well as changes in phenotypic properties and adherence. These Dex-mediated phenotypic alterations were furthermore associated with a metabolic switch in macrophages orchestrated via PKM2. Such treated macrophages lost their ability to prevent Aspergillus fumigatus germination, which was correlated with accelerated fungal growth, destruction of macrophages, and induction of an anti-inflammatory cytokine profile. Taken together, repolarization of macrophages following corticosteroid treatment and concomitant switch to an anti-inflammatory phenotype might play a prominent role in triggering invasive aspergillosis (IA) due to suppression of innate immunological responses necessary to combat extensive fungal outgrowth.
A Pilot Study on Baseline Fungi and Moisture Indicator Fungi in Danish Homes
Authors: Andersen Birgitte , Frisvad Jens C. , Dunn Robert R. , Thrane Ulf
Published: January 2021

In many complaint cases regarding bad indoor environments, there is no evidence of visible fungal growth. To determine if the problems are fungi-related, dust sampling is the method of choice among building surveyors. However, there is a need to differentiate between species belonging to a normal, dry indoor environment and species belonging to a damp building envelope. The purposes of this pilot study were to examine which fungal species are present in problem-free Danish homes and to evaluate different detection and identification methods. Analyses showed that the fungal diversity outside was different from the diversity inside and that the composition of fungal species growing indoors was different compared to those found as spores, both indoors and outdoors. Common for most homes were Pseudopithomyceschartarum, Cladosporiumallicinum and Alternaria sect. Infectoriae together with Botrytis spp., Penicilliumdigitatum and Pen. glabrum. The results show that ITS sequencing of dust samples is adequate if supported by thorough building inspections and that food products play as large a role in the composition of the baseline spora as the outdoor air and surrounding vegetation. This pilot study provides a list of baseline fungal species found in Danish homes with a good indoor environment.
Species Diversity, Mating Strategy and Pathogenicity of Calonectria Species from Diseased Leaves and Soils in the Eucalyptus Plantation in Southern China
Authors: Wu WenXia , Chen ShuaiFei
Published: January 2021

Many Calonectria species are causal agents of diseases on several forestry, agricultural and horticultural crops. Calonectria leaf blight is one of the most important diseases associated with Eucalyptus plantations and nurseries in Asia and South America. Recently, symptoms of leaf rot and leaf blight caused by Calonectria species were observed in a one-year-old Eucalyptus experimental plantation in GuangXi Province, southern China. To better understand the species diversity, mating strategy and pathogenicity of Calonectria species isolated from diseased tissues and soils, diseased leaves and soils under the trees from ten Eucalyptus urophylla hybrid genotypes were collected. Three hundred and sixty-eight Calonectria isolates were obtained from diseased Eucalyptus leaves and soils under these trees, and 245 representative isolates were selected based on the sampling substrates and Eucalyptus genotypes and identified by DNA sequence analyses based on the translation elongation factor 1-alpha (tef1), β-tubulin (tub2), calmodulin (cmdA) and histone H3 (his3) gene regions, as well as a combination of morphological characteristics. These isolates were identified as Calonectria hongkongensis (50.2%), C. pseudoreteaudii (47.4%), C. aconidialis (1.6%), C. reteaudii (0.4%) and C. auriculiformis (0.4%). This is the first report of C. reteaudii and C. auriculiformis occurrence in China. Calonectria pseudoreteaudii was isolated from both Eucalyptus diseased leaves and soils; the other four species were only obtained from soils. MAT1-1-1 and MAT1-2-1 gene amplification and mating type assignment results showed that C. pseudoreteaudii is heterothallic and an asexual cycle represents the primary reproductive mode, C. reteaudii and C. auriculiformis are likely to be heterothallic and C. hongkongensis and C. aconidialis are homothallic. Based on the genetic diversity comparisons for C. pseudoreteaudii isolates from diseased leaves and soils, we hypothesize that C. pseudoreteaudii in soils was spread from diseased leaves. Both the mycelia plug and conidia suspension inoculations indicated that all five Calonectria species were pathogenic to the two Eucalyptus genotypes tested and the tolerance of the two genotypes differed. It is necessary to understand the ecological niche and epidemiological characteristics of these Calonectria species and to select disease resistant Eucalyptus genotypes in southern China in the future.
Acknowledgment to Reviewers of Life in 2020
Authors:   Life Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Life maintains its standards for the high quality of its published papers [...]
Optimize Design of Run-Flat Tires by Simulation and Experimental Research
Authors: Liu Huaqiao , Pan Yiren , Bian Huiguang , Wang Chuansheng
Published: January 2021

In this study, the two key factors affecting the thermal performance of the insert rubber and stress distribution on the tire sidewall were analyzed extensively through various performance tests and simulations to promote the development of run-flat tires. Four compounds and two structures of insert rubber were designed to investigate the effects of heat accumulation and stress distribution on durability testing at zero pressure. It was concluded that the rigidity and tensile strength of the compound were negatively correlated with temperature. The deformation was a key factor that affects energy loss, which could not be judged solely by the loss factor. The stress distribution, however, should be considered in order to avoid early damage of the tire caused by stress concentration. On the whole, the careful balance of mechanical strength, energy loss, and structural rigidity was the key to the optimal development of run-flat tires. More importantly, the successful implementation of the simulations in the study provided important and useful guidance for run-flat tire development.
MooFuzz: Many-Objective Optimization Seed Schedule for Fuzzer
Authors: Zhao Xiaoqi , Qu Haipeng , Lv Wenjie , Li Shuo , Xu Jianliang
Published: January 2021

Coverage-based Greybox Fuzzing (CGF) is a practical and effective solution for finding bugs and vulnerabilities in software. A key challenge of CGF is how to select conducive seeds and allocate accurate energy. To address this problem, we propose a novel many-objective optimization solution, MooFuzz, which can identify different states of the seed pool and continuously gather different information about seeds to guide seed schedule and energy allocation. First, MooFuzz conducts risk marking in dangerous positions of the source code. Second, it can automatically update the collected information, including the path risk, the path frequency, and the mutation information. Next, MooFuzz classifies seed pool into three states and adopts different objectives to select seeds. Finally, we design an energy recovery mechanism to monitor energy usage in the fuzzing process and reduce energy consumption. We implement our fuzzing framework and evaluate it on seven real-world programs. The experimental results show that MooFuzz outperforms other state-of-the-art fuzzers, including AFL, AFLFast, FairFuzz, and PerfFuzz, in terms of path discovery and bug detection.
Physiological Balance of the Body: Theory, Algorithms, and Results
Authors: Alchinova Irina , Karganov Mikhail
Published: January 2021

Aim: To confirm algorithm of determination of risk groups with physiological imbalance in the population exposed to unfavorable anthropogenic influences. Methods: The testing included such functional systems as constitution, myocardial contractility, autonomic regulation of the heart rate, regulation of peripheral circulation, psychomotor regulation, respiratory regulation and metabolism. Monitoring is carried out using computerized measurement instrumentation and data processing systems. Results: A risk group with pronounced shifts in the physiological balance was identified, which made up 38% of the surveyed population. The greatest contribution to the imbalance was made by the psychomotor system. Conclusion: We analyzed two different components of organism’s adaptation: resistance and resilience. Physiological systems experiencing increasing load attain a tipping points, where even a weak disturbing influence can induce transition to a qualitatively different state. This transition can result in either recovery of the regulatory stability of the system, or its transition to a lower level (dysregulation) with further development of a pathology. In this regard, of paramount importance is early detection of the signals about approaching the tipping points, one of these is the slowing down phenomenon during functional tests. In view of intricate interaction of physiological systems, recording of as much indicators as possible is advisable. The method of partial correlations is effective for evaluation of adaptive interaction of systems.
Acknowledgment to Reviewers of Marine Drugs in 2020
Authors:   Marine Drugs Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Marine Drugs maintains its standards for the high quality of its published papers [...]