bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Clinical Success of Pediatric Fiberglass Crowns in Primary Teeth Using Different Luting Cements: A Randomized Controlled Trial
Authors: Bektaş Kübra , Çiftçi Z.Zahit , Karamete Büşra Buse , Karayılmaz Hüseyin
Published: October 2024

Aims
To evaluate the clinical success, impact on periodontal health, and parental satisfaction of pediatric fiberglass crowns (FGCs) applied to primary molars using two different luting cements.
Methods and Materials
In this study, 60 FGCs were applied to the primary molars of 38 children aged five-nine years, using two different cement materials [glass-ionomer cement (GIC) and resin-modified GIC (RMGIC)]. During the 12-month control period, the retention, anatomical integrity, effect on periodontal health and parental satisfaction of FGCs were evaluated. The plaque index (PI) and gingival index (GI) scores of all teeth, including those treated with crowns (FGC group) and their symmetrical, healthy counterparts (control group), were evaluated both before and after the application of FGCs.
Results
Although the highest survival was observed in the group cemented with RMGIC, there was no significant difference between the groups (p > 0.05). There was no statistically significant change in the periodontal index scores of the FGC group compared with the initial values (p > 0.05). There was a significant decrease in the periodontal index scores of the overall and control groups compared with the initial values (p < 0,05). When the gingival index scores were evaluated, the increase observed in the control sessions compared with the initial values is significantly greater (p ≤ 0.001).
Conclusion
At the end of the 12-month follow-up period, although the survival rate was higher in the RMGIC group, the clinical success rate of FGCs was low in general. It can be suggested that FGCs could serve as an aesthetic alternative for primary tooth crown restorations; however, improvements in their mechanical and surface properties are needed.
Trial Registration
The study was retrospectively registered on www.clinicaltrials.gov with registration number (NCT06558734).
The mediating role of anxiety in poor sleep quality and passive coping among adolescents
Authors: Xin Yongjuan , Zhao Shuxin , Mu Chunguang , Shang Yanna , Yang Wenjie , Hao Changfu
Published: October 2024

Background
The increasing prevalence of poor sleep quality in children and adolescents highlights the essential role of quality sleep in promoting growth and development. Coping styles are associated with cognitive and behavioral adaptations to stress. However, their connection to sleep quality remains unclear.
Methods
In the summer of 2023, a cross-sectional survey was carried out among middle school students in Henan Province, China. The students were randomly chosen from five cities within the province, each representing varying economic levels, using a stratified random sampling approach. The questionnaire comprised inquiries regarding participants’ demographic details, along with assessments of sleep quality, anxiety levels, and coping styles. The Pittsburgh Sleep Quality Index (PSQI) was employed to gauge the sleep quality of middle school students. The Self-Rating Anxiety Scale (SAS) was used to evaluate the anxiety levels of participants. Additionally, coping styles were assessed using the Simplified Coping Style Questionnaire (SCSQ). The correlation coefficients among these three variables were calculated. The significance of the mediation effect was obtained using a bootstrap approach with SPSS PROCESS macro.
Results
This study included 592 middle school students, 76 (12.8%) had a poor sleep quality, while 23.0% showed signs of anxiety. Additionally, 54.6% of students employed passive coping styles in their daily lives. Spearman correlations analysis showed that poor sleep quality was positively correlated with anxiety (r = 0.583, P < 0.01) and passive coping (r = 0.175, P < 0.01). Anxiety was positively correlated with passive coping (r = 0.206, P < 0.01). We validated the significance of the indirect effect of sleep quality mediated through anxiety(95% bootstrap CI = 0.048, 0.112). The percentage of indirect effect was calculated to be 84.4%. The mediating role of anxiety in passive coping and poor sleep quality was established and was fully mediated.
Conclusions
The mediating effect of anxiety on passive coping and poor sleep quality was confirmed and found to be fully mediated. Passive coping style is associated with inadequate sleep quality and the occurrence of anxiety symptoms. Assessing the correlation between sleep quality and coping style among middle school students is crucial for developing effective health promotion strategies to improve students’ health.
Impact of renal impairment on chemotherapy-induced neutropenia in patients who are treated with trifluridine/thymidine phosphorylase inhibitor + bevacizumab for metastatic colorectal cancer
Authors: Shibutani Masatsune , Tanda Hideki , Kasashima Hiroaki , Fukuoka Tatsunari , Kashiwagi Shinichiro , Maeda Kiyoshi
Published: October 2024

Although the phase III SUNLIGHT trial has demonstrated the survival benefit of the addition of bevacizumab (Bmab) to trifluridine/thymidine phosphorylase inhibitor (FTD/TPI), neutropenia, which frequently occurs during FDT/TPI + Bmab therapy, is a concern for clinicians. As TPI is excreted by the kidneys, the risk of adverse events is likely to be high in patients with an impaired renal function. This study aimed to investigate the relationship between renal impairment and the incidence of chemotherapy-induced neutropenia during FTD/TPI + Bmab therapy using real-world data. We retrospectively reviewed the medical records of 69 patients with metastatic colorectal cancer (mCRC) who were treated with FTD/TPI + Bmab for more than 28 days. Patients with renal impairment with an eGFR of 30–44 mL/min/1.73 m2 were defined as the G3b group. Seven patients (10.1%) were classified into the G3b group. Among the 69 patients enrolled in this study, grade ≥ 3 neutropenia was observed in 34 patients (49.3%), and grade 4 neutropenia was observed in 9 patients (13.0%). Patients in the G3b group had an approximately 24% higher incidence of grade ≥ 3 neutropenia in comparison to others (71.4% vs. 46.8%), and the incidence of grade 4 neutropenia in the G3b group was significantly higher than that in others (42.9% vs. 9.7%, p = 0.042). In an analysis limited to the G3b group, of the 5 patients who developed grade ≥ 3 neutropenia, four patients (80%) developed grade ≥ 3 neutropenia, and 2 (40%) developed grade 4 neutropenia within 30 days after initiation of FTD/TPI + Bmab therapy. However, the duration required for neutrophil count to recover to ≥ 1500 /mm3 and the treatment effects of the G3b group were comparable to those observed in other patients. FTD/TPI + Bmab therapy is associated with a high risk of severe neutropenia within 30 days of initiation, especially in patients with a decreased renal function.
Enhancing Th17 cells drainage through meningeal lymphatic vessels alleviate neuroinflammation after subarachnoid hemorrhage
Authors: Gao Dandan , Bin Zou , Zhu Kunyuan , Bi Shijun , Zhang Wenxu , Yang Xinyu , Lai Jieyu , Pan Pengyu , Liang Guobiao
Published: October 2024

Background
Subarachnoid hemorrhage (SAH) is a severe cerebrovascular disorder primarily caused by the rupture of aneurysm, which results in a high mortality rate and consequently imposes a significant burden on society. The occurrence of SAH initiates an immune response that further exacerbates brain damage. The acute inflammatory reaction subsequent to SAH plays a crucial role in determining the prognosis. Th17 cells, a subset of T cells, are related to the brain injury following SAH, and it is unclear how Th17 cells are cleared in the brain. Meningeal lymphatic vessels are a newly discovered intracranial fluid transport system that has been shown to drain large molecules and immune cells to deep cervical lymph nodes. There is limited understanding of the role of the meningeal lymphatic system in SAH. The objective of this research is to explore the impact and underlying mechanism of drainage Th17 cells by meningeal lymphatic on SAH.
Methods
Treatments to manipulate meningeal lymphatic function and CCR7-CCL21 pathway were administered, including laser ablation, injection of VEGF-C, gene knockout and protein injection. Mouse behavior was assessed using the balance beam experiment and the modified Garcia scoring system. Flow cytometry, enzyme-linked immunosorbent assays (ELISA), and immunofluorescence staining were used to study the impact of meningeal lymphatic on SAH drainage. Select patients with unruptured and ruptured aneurysms in our hospital as the control group and the SAH group, with 7 cases in each group. Peripheral blood and cerebrospinal fluid (CSF) samples were assessed by ELISA and flow cytometry.
Results
Mice with SAH showed substantial behavioral abnormalities and brain damage in which immune cells accumulated in brain. Laser ablation of the meningeal lymphatic system or knockout the CCR7 gene leads to Th17 cells aggregation in the meninges, resulting in a decreased neurological function score and increased levels of inflammatory factors. Injection of VEGF-C or CCL21 protein promotes Th17 cells drainage to lymph nodes, an increased neurological function score, and decreased levels of inflammatory factors. Clinical blood and CSF results showed that inflammatory factors in SAH group were significantly increased. The number of Th17 cells in SAH group was significantly higher than control group. Clinical results confirmed Th17 cells aggravated the level of neuroinflammation after SAH.
Conclusion
This study shows that improving the drainage of Th17 cells by meningeal lymphatics via the CCR7-CCL21 pathway can reduce brain damage and improve behavior in SAH mouse model. This could lead to new treatment options for SAH.
Pericallosal Artery Aneurysms: An Evidence-Based Analysis of Clinical Presentations, Therapeutic Approaches, and Outcome
Authors: Badary Amr , Alrefaie Khadeja , Azab Mohammed A. , Almealawy Yasser F. , Alibraheemi Mohammed Q. , Awuah Wireko Andrew , Hernández-Hernández Alan , Alrubaye Sura N. , Ansari Nabiha Midhat , Atallah Oday
Published: October 2024

Background:
Pericallosal artery aneurysms (PCAA) are relatively rare intracranial aneurysms that present unique challenges in diagnosis and management. This study provides a comprehensive review of the literature to assess demographic patterns, risk factors, treatment approaches, and complications associated with PCAA.
Methods:
Data from 23 studies were analyzed using Python with libraries such as Pandas and Matplotlib. Descriptive statistics and crosstabulations explored the relationships between treatment modalities (microsurgical, endovascular, combined) and complications, including hydrocephalus, vasospasms, and intraprocedural ruptures. Visualizations were employed to depict the prevalence and impact of various outcomes.
Results:
Analysis revealed a notable gender disparity, with females constituting 70.47% of the study population. The average age was 49.93 years, and the average aneurysm size was 6.34 mm. A majority of aneurysms were ruptured (542 ruptured vs. 251 unruptured). Risk factors like smoking were prevalent, and radiological features such as subarachnoid hemorrhage (SAH) were commonly reported. Endovascular treatment was slightly more frequent (86.96%) than microsurgical treatment (73.91%). Vasospasm was the most reported complication (56.5%), followed by hydrocephalus and intraprocedural rupture. The mortality rate was 6.82%, with a mean follow-up duration of 20.77 months.
Conclusion:
This review reveals that PCAAs predominantly affect females, with an average patient age of 49.93 years. Aneurysms averaged 6.34 mm and often caused SAH. Endovascular treatments were more common but had higher complication rates than microsurgical methods, which also carried risks. The mortality rate was 6.82%.
Adapted Physical Activity, Exergaming and Relaxation by biofeedback in Hematological intensive care unit – Study protocol of a Randomised Controlled Trial (APAER-H trial)
Authors: BOUSMIA Johanne , LANGLET Cécile , ELNAR Arpiné Ardzivian , GOETZ Christophe , BOLMONT Benoit , DORVAUX Véronique
Published: October 2024

Background:
Hematological malignancies and their treatments are known for their significant adverse effects on health-related quality of life (QoL). During high-dose treatments in Hematological Intensive Care Units (HICU), Adapted Physical Activity (APA) is recognised for its role in maintaining physical fitness and limiting fatigue. Psychological and emotional states are also impaired, with anxiety levels significantly increasing in this specific context. Limited information is available about this topic. However, APA has been shown to reduce anxiety in various population, including oncological patients. Furthermore, adding new technology as exergaming or Heart Rate Variability Biofeedback (HRVB) relaxation tools could be an effective way to regulate emotions during treatments while providing the health-benefits of APA. APA, Exergaming and Relaxation by biofeedback in Hematological intensive care units protocol is a randomised, controlled trial. Our study is designed to evaluate the effects of APA programs during high-dose treatments in HICU on anxiety, fatigue level, functional capacities, immune system activity, and global QoL. Additionally, we aim to analyse the added-value of using specific devices as Exergaming and HRVB relaxation on the aforementioned parameters. We expect a difference in effectiveness between the programs concerning emotional regulation.
Methods:
Ninety patients (18–75 years), with various forms of hematological malignancies admitted to HICU, with haematologist’ approval for APA, will be randomly allocated in a 1:1:1 ratio to three 3-week APA groups: APA only (APA), APA by Exergaming (EXER), APA + HRVB relaxation (BIO). APA sessions will consist of moderate aerobic training on cyclo-ergometer (classical stationary bicycle for APA, BIO and connected ergometer in EXER), 3 times per week. The HRVB training will consist of controlled breathing exercises with biofeedback of heart rate variability at the end of each APA session (BIO).
Discussion:
The primary outcome is to evaluate the effect of 3 short APA programs on state anxiety (HADS; STAI-YA) and fatigue (MFI-20). The secondary outcomes will assess the effects on physical fitness (2MWT; FTSST), QoL (EORTC-QLQC30) and immune system functioning (blood samples). All of these assessments are evaluated initially (T1) and directly after (T2).
Trial Registration:
APAER-H protocol (version 1.1 of the 14/06/2022) was approved by the French Sud Mediteranian III ethical committee and registered on ClinicalTrials.gov: NCT05475600 (https://clinicaltrials.gov/).
MulMed: Addressing Multiple Medical Tasks Utilizing Large Language Models
Authors: Cheng Nannan , Li Fangli , Huang Li
Published: October 2024

The proliferation of large-scale language models, such as ChatGPT, has underscored the urgent requirement to develop Language Models in Medicine (LLMs) to mitigate the burden on healthcare resources. This work introduces MulMed, a model that prioritizes multitasking capabilities in medical domains. MulMed aims to summarize complex medical texts, address patient inquiries, engage in medical question-answering dialogues, demonstrate cross-lingual proficiency, and offer comprehensive medical knowledge coverage. Its key contributions include a two-step fine-tuned modeling framework that enables the model to perform multi-task functions like medical text summarization and Q&A in both English and Chinese, demonstrating excellent generalization abilities on benchmark test sets. The model also exhibits human empathy in doctor-patient consultations, and its fine-tuning process and data are openly available to promote future research in cross-lingual medical models. Additionally, a medical ethics framework is proposed to aid in evaluating the feasibility of medical model applications.
Influence of hemodynamic risk stratification by American Heart Association (AHA) Level of Care (LoC) in Congenital Heart Disease (CHD) on location, mode of delivery and outcome parameters - a single center retrospective observational study
Authors: Hecht Tobias , Bergjan Mara , Bennemann Maike , Steinhard Johannes , Laser Kai Thorsten , Sandica Eugen , Schmitt Manfred , Schubert Stephan
Published: October 2024

Background
The aim of this analysis was to clarify the complexity of CHD in neonates, which are referred to a high-volume pediatric heart center. Does mode or place of delivery within different CHD types influence outcome parameters.
Methods
Observation period for this analysis was 10 years (2011–2021). All neonates younger 28 days with CHD were included and divided into 5 groups according to expected hemodynamic instability using the AHA Level of Care (LoC) Assignment.
Results
Of 1210 neonates, 825 met the inclusion criteria. 397 infants were delivered locally (group A). 428 neonates were transferred from distant hospitals (> 15 km) (group B). The predominant LoC grades were LoC 2 (46%) and LoC 1 (28%). LoC grades 3, 4 and P (palliative care) accounted for 26%. A prenatal diagnosis was available in 54% of cases. The concordance rate with postnatal diagnosis was 88%. 92% of locally delivered infants had a prenatal diagnosis, but only 19% of transferred newborns. There were no differences in mode of delivery, birth weight, and APGAR scores between the different delivery locations. The proportion of univentricular heart defects was 51% in group A at LOC 2 and only 24% in group B. The overall mortality rate was 8.4% at LOC 2, with the highest mortality rate of 12.8% at LOC 4.
Conclusion
25% of our neonates fulfilled the LoC criteria of an increased risk for hemodynamic instability (LoC 3–4). Comparative studies show no differences in perinatal outcomes. Newborns with single ventricle morphology have an increased mortality rate.
The moderating role of workforce participation on the association between inflammatory diet and depression among US adults: findings from NHANES 2005-2018
Authors: Sun Zhenyu , Tang Haoru , Gao Hongliang , Chen Xi , Qian Dongfu
Published: October 2024

Background Depression in the workforce is a highly prevalent and prominent public health problem with pro-inflammatory diet. We aimed to assess the association between inflammatory diet and depression, then to further examine the moderating role of workforce participation on the association of inflammatory diet with depression among US adults aged 20 years or above. Methods Data from the National Health and Nutrition Examination Survey (NHANES) 2005–2018 was used. Binary logistic regression and moderating effect analysis were conducted to explore the associations between inflammatory diet, workforce participation, and depression. Restricted cubic spline (RCS) were employed to evaluate the dose–response association of dietary inflammatory index (DII) and hours worked per week with depression at different levels. Results A total of 23,163 participants were enrolled with a depression prevalence of 7.4%. Both pro-inflammatory diet and not working were risk factors for depression. After adjusting for covariates, adults with pro-inflammatory diet (DII ≥ 0) were more likely to suffer from depression (OR = 1.30; 95%CI: 1.10–1.55). Workforce participation moderated the association between inflammatory diet and depression, especially moderating effect of not working and regular daytime working on the association of DII with depressive tendency was 0.12 and − 0.06. The protective effect of anti-inflammatory diet against depression was only found during the working period of 25 to 45 hours peer week. These effects were consistent across the analyses of subgroup and sensitivity. Conclusions Not working may exacerbate the increase of pro-inflammatory diet to depression risk. Modest regular daytime working (25–45 hours per week) with anti-inflammatory diet may better in reducing depression risk.
Numerical simulation of a metallurgical transformation furnace using residual mixtures of industrial and automotive oils with added maintenance solvents
Authors: Figueroa Martha Angélica Cano , Hugo Arcos Gutiérrez , Isaías Garduño , Raúl Pérez Bustamante , Moreno Juan R.
Published: October 2024

This article presents the exciting results of a numerical analysis of combustion and energy efficiency parameters in an IDR (adjustable direct injection) oscillating furnace, used in a combined metallurgical process and operated with a unique blend of automotive, industrial, and aggregate oils. The simulation process kicks off with the design of the real-world CAD (computer-aided design) environment, the programming in CFD (Computational Fluid Dynamics), and the validation in field-based PAT (Process Analytical Technology) experiments. This methodology was a total game-changer! It allowed us to predict the injection behavior of the mixture, as well as the temperature ranges, wind tunnel effects within combustion, pressures in the working zones, and heat release. We then compared the data with the experimental scenario, which was a great way to measure the efficiency and performance of the mixture, observe the thermal combustion behaviour within the oven, and compare the results with other fuels commonly used in this type of transformation. The experimental results were astounding! The thermal performance was found to be a whopping 73% higher than other fuels and combined cycles. The efficiency improvement was even more impressive, reaching 84.6%. That means the process is not only more efficient, but it's also 54.76% faster than traditional times with fossil fuels. And that's not all! The cost reduction was an incredible 76.43%. It also meant an affectation of 10.5% since these must be adjusted to the requirements of the process terms. The simulated and experimental results show the thermal ranges, fuel efficiency, percentage efficiency of the cycle, implications in the process tasks, costs and impact of migration by fuel type. The deviation between experimental and simulation values of process temperatures on a common fuel is diesel or gas, showed the advantages of using predictive tools in the use of reidual fuels and the importance of the conservation of the aggregates to the mixtures. The CFD analysis together with experimental studies proved to be an effective method to predict the process parameters inside the furnace operating with new energy sources fueled with waste blends.
From Sprouting to Senescence: Phenological Chronicles of Phytolacca acinosa Roxb. in the Himalayan Highlands
Authors: Magray Junaid A. , Wani Bilal A. , Islam Tajamul , Javid Hanan , Ganie Aijaz Hassan , Qadir Rouf Ul , Nawchoo Irshad A.
Published: October 2024

In the era of the Anthropocene, changes in the timing of plant life cycle events serve as prominent biological markers of global environmental shifts. Major of the studies dedicated to documenting and analyzing plant phenological changes are from North America and Europe. Although few studies are available from Himalayan regions, but there is a notable scarcity of comparable studies in the developing world, particularly in regions like the Himalaya. To fill this knowledge gap, the present study aims to attempt the reveal to attempt to unravel the vegetative and reproductive phenology of Phytolacca acinosa across Kashmir Himalaya. For this, we selected and tagged 10 healthy individuals for monitoring and documenting the onset and duration of different phenological events from sprouting to senescence. Further, flowering phenology was recorded using flowering variables such as moment, intensity and duration. Our results reveal that Phytolacca acinosa completes the entire life cycle within a period 7–8 months, and the timing of phenophases varied significantly across the selected sites. Our results also reveal that the plant populations at lower elevations exhibited a clear precedence of vegetative and reproductive phases in their life cycle as compared to those growing at higher elevations. We found that all the phenophases except senescence showed an overlap with the preceding phase. We also found that the flowering variables showed significant variation across the selected sites with the maximum values at low elevations, while minimum values at high elevations. Our study is the first attempt that provides useful insights into the phenology of P. acinosa across different habits along an elevation gradient in Kashmir Himalaya. Phenological adjustments, such as advancing or delaying reproductive phases, are crucial strategies for species to optimize reproductive success. Global climate change, characterized by rapid warming and disruptions, particularly during reproductive periods, has notably impacted species at higher elevations. Understanding phenological attributes is vital in contemporary conservation biology and the phenological data from this study will assist in formulating effective strategies for the restoration and conservation of the target species.
Gli1+ Inflamm-Stem Cells Engage Extracellular Vesicles to Prime Aberrant Neutrophils for Exacerbating Periodontal Immunopathology
Authors: Jin Yan , Cai Xin-Yue , Zheng Chen-Xi , Guo Hao , Fan Si-Yuan , Huang Xiao-Yao , Chen Ji , Liu Jie-Xi , Gao Yu-Ru , Liu An-QI , Liu Jia-Ning , Zhang Xiao-Hui , Ma Chao , Wang Hao , Fu Fei , Peng Peng , Xu Hao-Kun , Sui Bing-Dong , Xuan Kun
Published: October 2024

Periodontitis is a prevalent and progressive detrimental disease which is characterized by chronic inflammation, the immunopathological mechanisms being not yet fully understood. Mesenchymal stem cells (MSCs) play crucial roles as immunoregulators and maintain tissue homeostasis and regeneration, but their in vivo function in immunopathology and periodontal tissue deterioration is still unclear. Here, we utilized multiple transgenic mouse models to specifically mark, ablate and modulate Gli1+ cells, a critical and representative subset of MSCs in the periodontium, to explore their specific role in periodontal immunopathology. We reveal that Gli1+ cells, upon challenging by an inflammatory microenvironment, significantly induce rapid trafficking and aberrant activation of neutrophils thus exacerbating alveolar bone resorption. Mechanistically, extracellular vesicles (EVs) released by Gli1+ cells act as crucial immune regulators in the periodontal tissue, mediating the recruitment and activation of neutrophils through increased generation of reactive oxygen species and trigger of the nuclear factor kappa-B signaling. Importantly, specific inhibition of EV release from Gli1+ cells or pharmacological therapy using GANT61 ameliorates periodontal inflammation and alveolar bone loss. Collectively, our findings identify previously unrecognized roles of Gli1+ cells in orchestrating infiltration and priming aberrant activation of neutrophils under inflammation, which provide pathological insights and potential therapeutic targets for periodontitis.
NCAPH promotes glucose metabolism reprogramming and cell stemness in ovarian cancer cells through the MEK/ERK/PD-L1 pathway
Authors: Qi Yingying , Chen Wei , Wang Aiping , Chen Silin
Published: October 2024

Backgrounds:
Ovarian cancer is a prevalent malignant tumors affecting the female reproductive organs with the characteristic of high heterogeneity. Non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) has been implicated in a variety of cancers.
Methods
The expression of NCAPH before or after transfection was detected using RT-qPCR and western blot. Cell stemness was assessed with spheroid formation assay. The extracellular acidification rate (ECAR) of ovarian cancer cells was appraised utilizing Seahorse Glycolysis Stress Test Assay while oxygen consumption rate (OCR) was estimated with Seahorse Mito Stress Test Assay. Lactate production and glucose consumption were evaluated with corresponding assay kits. Western blot was adopted to evaluate the contents of stem cell markers, glycolysis- and MEK/ERK/PD-L1 signaling pathway-related proteins. In vivo, the tumor size and weight were measured and KI67 expression in tumor tissues of nude mice was appraised utilizing immunohistochemical staining.
Results
It was found that NCAPH expression was upregulated in ovarian cancer cells. After silencing NCAPH expression, the stemness and glucose metabolism reprogramming were repressed. The MEK/ERK/PD-L1 signaling pathway was inhibited by NCAPH knockdown both in vitro and in vivo. NCAPH depletion was also discovered to suppress tumor growth in mice.
Conclusion
Collectively, NCAPH silence impeded the malignant progression of OC through the MEK/ERK/PD-L1 pathway.
Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana.
Authors: Ueda Minoru , Saito Rina , Nishizato Yuho , Kitajima Tsumugi , Kato Nobuki
Published: October 2024

(+)-cis-12-oxo-phytodienoic acid (cis-OPDA) is a biosynthetic precursor of the plant hormone (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile). It functions as an endogenous chemical signal independent of the JA-Ile receptor COI1-JAZ in Arabidopsis thaliana. The bioactive form of cis-OPDA that induces COI1-JAZ-independent gene expression remains unknown.
In this study, we demonstrated that the genuine bioactive forms of cis-OPDA are the downstream metabolites of b-oxidation, which upregulate the expression of the OPDA marker genes such as ZAT10and ERF5 in a COI1- or JA-Ile-independent manner. These downstream metabolites function independently of the JA-Ile-COI1-JAZ-MYCs canonical jasmonate signaling module, and their electrophilic nature is essential for their bioactivity.
Mobility Infrastructure Optimization via Stochastic Relaxation the Traffic Flow Reconstruction
Authors: Bilotta Stefano , Collini Enrico , Fanfani Marco , Nesi Paolo
Published: October 2024

Urban traffic congestion and pollution represent critical challenges for sustainable city development. In this paper, we presents a novel approach to optimizing urban mobility infrastructure using a stochastic relaxation algorithm. The method is designed to address the growing challenges of traffic congestion, fuel consumption, and CO2 emissions in rapidly urbanizing cities. The system automates the generation and evaluation of road network modifications, drastically reducing the time and effort required for traditional simulation-based approaches. By utilizing real-time traffic data and traffic flow reconstruction algorithms within the Snap4City platform, the proposed system allows for the efficient exploration of "what-if" scenarios, optimizing key performance indicators such as travel time, fuel use, and emissions. A case study conducted in Florence, Italy, demonstrates significant improvements in traffic conditions, fuel efficiency, and emissions reduction, underscoring the system's potential to enhance urban mobility in a sustainable manner. This system has been developed exploiting the Snap4City platform Scenario Editor and platform.
Heterogeneity of depression and anxiety among college students and related risk and protective factors: a network model
Authors: Niu Chunjuan , Jiang Yaye , Li Yihui , Wang Xudong , Zhao Huiyuan , Cheng Zhengshu , Li Xiaoran , Zhang Xu , Liu Zhiwei , Yu Xiaoyu , Peng Yan
Published: October 2024

Background
Comorbidity of depression and anxiety is common among adolescents and can lead to adverse outcomes. However, there is limited understanding of the latent characteristics and mechanisms governing these disorders and their interactions. Moreover, few studies have examined the impacts of relevant risk and protective factors.
Methods
This cross-sectional study involved 1,719 students. Mplus 8.0 software was used to conduct latent profile analysis to explore the potential categories of depression and anxiety comorbidities. R4.3.2 software was used to explore the network of core depression and anxiety symptoms, bridge these disorders, and evaluate the effects of risk and protective factors.
Results
Three categories were established: “healthy” (57.8%), “mild depression-mild anxiety” (36.6%), and “moderately severe depression-moderate anxiety” (5.6%). “Depressed mood”, “nervousness”, and "difficulty relaxing" were core symptoms in both the depression-anxiety comorbidity network and the network of risk and protective factors. Stress perception and neuroticism serve as bridging nodes connecting some symptoms of depression and anxiety and are thus considered the most prominent risk factors.
Conclusions
According to the core and bridging symptoms identified in this study, targeted intervention and treatment can be provided to groups with comorbid depression and anxiety, thereby reducing the risk of these comorbidities in adolescents.
Meeting climate target with realistic demand-side policies in the residential sector
Authors: VIVIER Lucas , Mastrucci Alessio , Ruijven Bastiaan van
Published: October 2024

The EU has established an ambitious policy framework for demand-side mitigation in buildings towards net-zero targets. Here, we conduct a comprehensive quantitative assessment of 384 demand-side policy combinations for residential space heating that complement supply-side decarbonization efforts. We show that the implementation of EU Emissions Trading System 2, even when combined with deep decarbonization of energy supply, falls short of climate targets. Beyond ETS 2, we emphasize the need for ambitious subsidies for heat pumps as a critical component of a successful strategy. Conversely, a large-scale generic ‘Renovation Wave’ modestly contributes to decarbonization, is not a cost-effective strategy at the EU level and requires significant increases in public spending. We advocate for the implementation of a carbon tax, paired with substantial subsidies for heat pumps and targeted incentives for home insulation by country and building. This approach supports the decarbonization of the residential sector, limits the strain on the electricity grid, and alleviates energy poverty.
The increased effect of spring leaf unfolding on autumn senescence in the northern and southern hemispheres
Authors: Deng Jianming , Tang Dong , Xie Shubin , Peng Jie , Sun Ying , Degen Abraham Allan , Sun Yuan , Hu Weigang , Dong Longwei , Hou Qingqing , Dong Xiaobing , Zhang Liang , Luo Jiali , Ran Jinzhi , Fu Yongshuo
Published: October 2024

Plant phenology, the timing of periodic events in plant development, is an indicator for detecting climate-vegetation dynamics. Although the importance of vegetation growth carryover (VGC) on phenology was recognized in the Northern Hemisphere (NH), it is unclear how VGC and climatic factors contribute to phenology and how these contributions evolve at a global scale. Utilizing two sets of satellite NDVI data, we explored the impacts of climate change and VGC on start-of-season (SOS) and end-of-season (EOS) plant phenology during the past four decades. Here we show that, globally, advanced SOS resulted primarily from the increasing temperature and radiation, whereas delayed EOS was mainly attributed to the increasing temperature and VGC in SOS (VGCSOS). VGCSOS was the main driver of EOS in the southern hemisphere (SH), whereas it was temperature in the NH. Furthermore, the contribution of VGCSOS to EOS displayed increasing trends over the past four decades in both hemispheres, which was particularly significant in NH. These differences were due primarily to the more evident "warming and drying" trends in the SH than NH, which weaken VGCSOS's impact on vegetation EOS. We conclude that VGCSOS contributes substantially to EOS in both hemispheres, offering insights for accurate predictions of vegetation growth and carbon sequestration under future global warming scenarios.
Exploring Mechanistic Insights and Apoptotic Gene Expression Induced by Myricetin Through In-Vitro and In-Silico Approaches
Authors: Nishad Khushbu , Usha Talambedu , Kumar H.P. Prashanth , Hemavathi K.N. , Joseph Albin , Ramesh N. , Middha Sushil Kumar
Published: October 2024

Myricetin demonstrates considerable potential in the area of anticancer research. It is known to be recognized as a rich natural polyphenolic flavonoid found abundantly in diverse dietary sources. This study investigates the impact of myricetin on cellular processes, including cell death, cell cycle regulation, and the expression of apoptotic genes in MCF-7 breast cancer cells. The cytotoxicity of myricetin was assessed using the MTT assay and cell cycle analysis on MCF-7 cells. Propidium iodide or Annexin V flow cytometric analysis was used to confirm apoptosis. Quantitative real-time PCR was used to measure the gene expression linked to apoptosis in MCF-7 cells following a 24-hour myricetin administration. Molecular docking was employed to probe the binding of myricetin to BCL-2. The cytotoxicity of myricetin revealed an IC50 of 5.06 µg/100 µl at 24 hours. Myricetin induced apoptosis in the Sub G0/G1 phase (10.38%). The findings showed up-regulation of the antiapoptotic gene BCL-2, along with elevated expression of caspase-9 and caspase-7, while the caspase-3 gene exhibited down-regulation. In silico analysis further confirmed the efficient binding of myricetin to BCL-2. The findings suggest that Myricetin exhibits a potent apoptotic effect by inhibiting cell cycle progression, however, further in-vivo investigations are necessary to understand the complexity of this molecule.
NINJ1 regulates plasma membrane fragility under mechanical tension
Authors: Xu Jie , Zhu Yunfeng , Xiao Fang , Wang Yiling , Wang Yufang , Li Jialin , Zhong Dongmei , Huang Zhilei , Yu Miao , Wang Zhirong , Barbara Joshua , Plunkett Christopher , Zeng Mengxue , Song Yiyan , Wang Zhongxing , Cai Changjie , Guan Xiangdong , Hammack Scott , Zhang Liang , Shao Feng , Shi Zheng , Xiang Fu-li
Published: October 2024

Plasma membrane integrity is vital not only for cell survival but also nearly all aspects of cell functioning. Mechanical stress can cause plasma membrane damage, but it is not known whether there are large molecules (proteins) that control plasma membrane integrity. Here we constructed a 384-well cellular stretch system that delivers precise, reproducible mechanical strain to adherent cells. Using the system, we screened 10,843 siRNAs targeting 2,726 multi-pass transmembrane proteins for stretch-induced membrane permeability changes. The screen identified NINJ1, a protein recently proposed to regulate pyroptosis and other lytic cell death, as the top hit. We demonstrate that NINJ1 is a critical regulator for mechanical force-induced plasma membrane rupture (PMR), without the need of stimulating any cell death programs. Low NINJ1 expression renders the membrane more resistant to stretching, while high expression of NINJ1 lowers the threshold of PMR under mechanical strain. NINJ1 level on the plasma membrane is inversely correlated to tension required to rupture the membrane. In the pyroptosis context, NINJ1 on its own is not sufficient to fully rupture the membrane, and additional mechanical stress is required for full PMR. Our work establishes that NINJ1 functions as a bona fide determinant of membrane biomechanical properties. Our study also suggests that PMR across tissues of distinct mechanical environments is subjected to fine tuning by differences in NINJ1 expression and external mechanical forces.
Unsupervised Multi-Source Domain Adaptation Person Re-Identification Methods Based on Transformer and Graph Convolutional Neural Network
Authors: Li Hao , Jia Minghui , Yu Jiexiao , Zhao Xin , Zhang Tao
Published: October 2024

The Person Re-Identification expects to retrieve the unique pedestrians across multiple non-overlapping cameras. Mainstream unsupervised person Re-Identification methods generate pseudo-labels by clustering in single-source and single-target domain scenarios. Although convolution neural network (CNN)-based methods have achieved promising progress, existing methods loss some feature information during convolution and downsampling operators. Moreover, due to the dramatic disparities between different source datasets, the model trained on the multi-source domain directly will lead to poor performance. To overcome these limitations, we propose a unsupervised multi-source domain adaptation person Re-Identification method based on transformer and graph convolutional neural network. Firstly, we introduce a pure transformer framework for Re-ID task to improve the model's feature representation capability. Secondly, we suggest using domain information fusion module based on graph convolutional neural network to minimize the gap among the source domains, and employ a feature enhancer based on Gaussian distribution to reduce domain-specific characteristics and increase the distinctiveness of person features. Finally, extensive experiments on four benchmark datasets show that the proposed method outperforms most of the current unsupervised domain adaptation person Re-Identification methods.
WITHDRAWN: Relationship Between Glycemic Control, Psychological Health, and Lower Extremity Functional Impairments in Post-stroke Diabetic Patients
Authors: Rauf Tooba , Shabbir Fatima , Tariq Aatika , Eman Kanzul , Jahan Saira , Ghous Misbah
Published: October 2024

Background
Understanding the interplay of the psychological and physical challenges faced by post-stroke diabetic patients is essential for developing comprehensive treatment strategies to improve their overall well-being and quality of life. Limited studies examine the association between the variables hence, our study aims to determine the relationship between glycemic control, psychological health, and lower extremity functional impairments in post-stroke diabetic patients.
Methods
A correlational study was conducted involving 214 post-stroke diabetic participants including both genders aged 50 years and above, recruited in the rehabilitation department of hospitals in the twin cities of Islamabad and Rawalpindi. The data collected from the patients included glycemic control (HbA1c, BSR, and BSF), psychological health assessment using DASS-21, and lower extremity functionality assessment using FMA-LE. Correlation and multiple regression were performed between the variables using IBM Statistics SPSS v 25.0.
Results
Out of 214 participants, the frequency of males was n = 119 (55.6%), and females was n = 95 (44.5%). The mean HbA1c calculated was 10.88 ± 1.37 mmol/mol. A positive and significant (p < 0.05) correlation between glycemic control and psychological health was depicted except for BSR with stress. Similarly, a negative and significant (p < 0.05) correlation was found between glycemic control and lower extremity functionality except for BSF with sensation. The correlation between glycemic control and lower extremity functionality showed a negative and significant (p < 0.05) relationship except for anxiety with sensation and passive joint motion, and stress with motor function. Additionally, a strong association was observed between glycemic control with anxiety, stress, and sensation, and between depression and lower extremity.
Conclusion
This study reveals a complex interaction between glycemic control, lower extremity functionality, and psychological health. Findings indicate that poor glycemic control is associated with increased psychological distress and impaired lower extremity function. Lower extremity impairments can eventually lead to poor psychological health in post-stroke diabetic patients.
Mesenchymal Stem Cell Secretome Effectiveness on Healing of Chronic Tendon Injury: Analysis of Procollagen Type I N- Terminal Peptide and Procollagen Type Iii N-terminal Peptide and Histopathology in Rat’s Tendon (Rattus Norvegicus)
Authors: Sam Andi Dhedie Prasatia , Warsinggih Warsinggih , Usman Muhammad Andry , Johan Muhammad Phetrus , Suroto Heri , Saleh M. Ruksal , Sakti Muhammad , Zainuddin Andi Alfian , Mubarak Andi Firman
Published: October 2024

Background
Chronic tendon injuries, such as Achilles tendinopathy, are common and challenging to treat due to the limited regenerative capacity of tendon tissue. Using mesenchymal stem cell (MSC) secretome, which contains a rich array of growth factors, holds promise for enhancing tendon healing. This study aimed to evaluate the effectiveness of MSC secretome, derived from tendon-derived stem cells (TDSCs) and adipose-derived stem cells (ASCs), on the healing of chronic Achilles tendon injuries in a rat model, focusing on the levels of Procollagen Type I N-Terminal Peptide (PINP) and Procollagen Type III N-Terminal Peptide (PIIINP), and histopathological changes.
Methods
A chronic tendinopathy model was induced in 16 males of Rattus norvegicus via mechanical overloading and collagenase injection. Rats were divided into four groups: TDSC secretome, ASC secretome, combined TDSC + ASC secretome, and a control group. Secretomes were administered intratendinously. Tendon healing was assessed after four weeks using enzyme-linked immunosorbent assays (ELISA) to measure PINP and PIIINP levels and histopathological analysis to evaluate collagen deposition and tissue structure.
Results
PINP levels were significantly higher in the TDSC + ASC group compared to the control group (p = 0.004), indicating enhanced Type I collagen synthesis. However, no significant differences were observed in PIIINP levels between the groups. The histopathological analysis did not reveal significant structural differences in tendon healing among the groups, though increased collagen alignment was observed in the TDSC + ASC group.
Conclusions
The combined TDSC and ASC secretome promotes Type I collagen synthesis in chronic tendon injuries, but histological improvements were insignificant. Further studies are needed to confirm the long-term benefits of secretome therapy.
On the Reliability of Large Language Models to Misinformed and Demographically-Informed Prompts
Authors: Aremu Toluwani Samuel , Akinwehinmi Oluwakemi , Nwagu Chukwuemeka , Ahmed Syed Ishtiaque , Orji Rita , Amo Pedro Arnau Del , Saddik Abdulmotaleb El
Published: October 2024

We investigate and observe the behaviour and performance of Large Language Model (LLM)-backed chatbots in addressing misinformed prompts and questions with demographic information within the domains of Climate Change and Mental Health. Through a combination of quantitative and qualitative methods, we assess the chatbots’ ability to discern the veracity of statements, their adherence to facts, and the presence of bias or misinformation in their responses. Our quantitative analysis using True/False questions reveals that these chatbots can be relied on to give the right answers to these close-ended questions. However, the qualitative insights, gathered from domain experts, shows that there are still concerns regarding privacy, ethical implications, and the necessity for chatbots to direct users to professional services. We conclude that while these chatbots hold significant promise, their deployment in sensitive areas necessitates careful consideration, ethical oversight, and rigorous refinement to ensure they serve as a beneficial augmentation to human expertise rather than an autonomous solution.
A Study of an EPQ Model of Deterioration Items with Effect of Carbon Emission under Pentagonal Intuitionistic Fuzzy Number
Authors: Dey Agniva , Ray Partha , Seal Somen
Published: October 2024

These days, a lot of inventory experts focus especially on green inventory challenges,
which include global carbon emission difficulties. This study explores a green
inventory EPQ model in which the selling price, inventory level, and degree of green
awareness determine the demand rate. The use of conservation technology has helped
to control carbon emissions; the holding cost is assumed to be a quadratic function of
time, while the deterioration cost is assumed to be a linear function of time. Initially, a
crisp model for minimizing average total cost is developed. Then, if the idea of

membership value and nonmembership value exists, the demand rate assumes

unpredictability in nature as a result of market versatility. Therefore, a fuzzy model is
developed with the assumption that the pentagonal intuitionistic dense fuzzy number
represents the demand rate. To solve the suggested model, a defuzzification method
and a solution algorithm have been developed. A comparative analysis is provided

between crisp and various fuzzy environments in the numerical demonstration.
Moreover, LINGO 21.0 software has been used for numerical computing. Lastly,
sensitivity analysis and graphical depiction have been used to support the proposed
model.