bluuebunny/crossref_metadata_2025 huggingface dataset:
Information Theoretic Modeling of High Precision Disparity Data for Lossy Compression and Object Segmentation
Authors: Tăbuş Ioan , Kaya Emre Can
Published: November 2019

In this paper, we study the geometry data associated with disparity map or depth map images in order to extract easy to compress polynomial surface models at different bitrates, proposing an efficient mining strategy for geometry information. The segmentation, or partition of the image pixels, is viewed as a model structure selection problem, where the decisions are based on the implementable codelength of the model, akin to minimum description length for lossy representations. The intended usage of the extracted disparity map is to provide to the decoder the geometry information at a very small fraction from what is required for a lossless compressed version, and secondly, to convey to the decoder a segmentation describing the contours of the objects from the scene. We propose first an algorithm for constructing a hierarchical segmentation based on the persistency of the contours of regions in an iterative re-estimation algorithm. Then, we propose a second algorithm for constructing a new sequence of segmentations, by selecting the order in which the persistent contours are included in the model, driven by decisions based on the descriptive codelength. We consider real disparity datasets which have the geometry information at a high precision, in floating point format, but for which encoding of the raw information, in about 32 bits per pixels, is too expensive, and we then demonstrate good approximations preserving the object structure of the scene, achieved for rates below 0.2 bits per pixels.
Digital Literacy of Teachers in Training: Moving from ICTs (Information and Communication Technologies) to LKTs (Learning and Knowledge Technologies)
Authors: Gómez-Trigueros Isabel María , Ruiz-Bañuls Mónica , Ortega-Sánchez Delfín
Published: November 2019

This study is based on the need to work on the digital literacy of our Infant Education degree, Primary Education degree and Master in Secondary Education students so that, as future teachers, they are able to make the necessary transition from ICTs (Information and Communication Technologies) to LKTs (Learning and Knowledge Technologies) Through a mixed methodology, knowledge and perceptions of basic technological concepts and tools of our trainee teachers are identified. The instrument used has been a Likert scale questionnaire, adapted and validated by experts from the participating universities. Its internal consistency demonstrates its worth and functionality for the proposed analysis (α = 0.958). The first results show a clear lack of knowledge of certain technological concepts essential for their future teaching work and, in turn, show significant differences regarding the knowledge of ICTs according to the age of the participants.
Robust Two-Layer Model Predictive Control for Full-Bridge NPC Inverter-Based Class-D Voltage Mode Amplifier
Authors: Wei Xinwei , Wang Hongliang , Wang Kangliang , Li Kui , Li Minying , Luo An
Published: November 2019

Finite control set model predictive control (FCS-MPC) is able to handle multiple control objectives and constraints simultaneously with good dynamic performance. However, its industrial application is limited by its high dependence on system model and the huge computational effort. In this paper, a novel robust two-layer MPC (RM-MPC) with strong robustness is proposed for the full-bridge neutral-point clamped (NPC) voltage mode Class-D amplifier (CDA) aiming at this problem. The errors caused by the parameter mismatches or uncertainties of the LC filter and the load current are regarded as lumped disturbance and estimated by the designed Luenberger observer. The robust control can be achieved by compensating the estimated disturbance to the used predictive model. In order to reduce computation of the controller, a two-layer MPC is proposed for the full-bridge NPC inverter with an LC filter. The first layer is used to calculate the optimal output level which minimizes the tracking error of the output voltage. The second layer is used to determine the switching state for the purpose of capacitor voltage balancing. The experimental results show that the lumped model error is observed centrally through only one observer with low complexity. The two-layer MPC further reduced the computation without affecting the dynamic performance.
Scheduling Fair Resource Allocation Policies for Cloud Computing through Flow Control
Authors: Souravlas Stavros , Katsavounis Stefanos
Published: November 2019

In this short paper, we discuss the problem of resource allocation for cloud computing. The cloud provides a variety of resources for users based on their requirements. Thus, one of the main issues in cloud computing is to design an efficient resource allocation scheme. Each job generated by a user in the cloud has some resource requirements. In this work, we propose a resource allocation method which aims at maximizing the resource utilization and distributing the system’s resources in a fast and fair way, by controlling the flow according to the resources available and by analyzing the dominant demands of each job. Moreover, by parallelizing the computations required, the runtime of the proposed strategy increases linearly as the number of jobs N increases. Here, we present some initial experimental results for small sets of users, that have shown that our strategy allocates the available resources among user jobs in a fair manner, while increasing the overall utilization of each resource.
Validating Performance Models for Hybrid Power Plant Control Assessment
Authors: Petersen Lennart , Iov Florin , Tarnowski German Claudio , Gevorgian Vahan , Koralewicz Przemyslaw , Stroe Daniel-Ioan
Published: November 2019

The need for simple, but accurate performance models of wind turbine generators (WTGs), photovoltaic (PV) plants, and battery energy storage systems (BESS) for various hybrid power plant (HPP) studies motivates the present work. Particularly, the development and verification stage of HPP controls requires reduced-order models to minimize the complexity and computation effort of simulation platforms. In this paper, such models are proposed, and the most essential parts of the models are validated through field measurements. The models target power system integration studies involving active and reactive power, as well as frequency and voltage regulation where detailed models, as proposed in the standards, can be cumbersome. Field measurements of two Vestas WTGs, one 1-MW PV plant, and one 1-MW/1-MWh BESS are used for model validation. The results show that the WTG and PV performance models correctly estimate the power generation variability according to fluctuations in wind speed and solar irradiance. The BESS performance model provides satisfactory results related to grid-forming control performance and estimation of state-of-charge. The presented validation work enables using the proposed performance models for power system studies and HPP control design in all model-based design stages, that is, preliminary analysis, design, verification, and validation with a high level of confidence.
A Simple Multilevel Space Vector Modulation Technique and MATLAB System Generator Built FPGA Implementation for Three-Level Neutral-Point Clamped Inverter
Authors: Madasamy P. , Pongiannan R. K. , Ravichandran Sekar , Padmanaban Sanjeevikumar , Chokkalingam Bharatiraja , Hossain Eklas , Adedayo Yusuff
Published: November 2019

The pulse width modulation (PWM) is an important segment in power electronic inverters and multilevel inverters (MLIs) design. The space vector modulation (SVM) methods own distinct advantages over other PWM methods. However, MLI SVM has involved more mathematics in their executions. Hence, the digital signal processors (DSPs) or field programmable gate arrays (FPGAs) based digital implementations are highly preferred for MLI SVM realizations, which require exceptional properties. The conventional MLI SVMs use complex mathematical functions to solve their internal functions to identify the space vector diagram (SVD) sub-triangle and over modulation boundary switching on-times. Particularly these are the changes in the position of reference vector with respect to their sub-triangle positions involving higher mathematical functions. This paper proposes a simplified three-level MLI SVM that reduces the sub-triangle and over modulation switching on-time calculations with reduced mathematical functions. The proposed MLI SVM is derived based on two-level SVM without changing the reference vector position, unlike the traditional approaches. This helps in extending the SVM for any n-level inverter with additional LUTs. The detailed theoretical study, MATLAB-Simulink system generator simulations and Xilinx FPGA family SPARTAN-III-3A based experimental implementations are done with three-level neutral point MLI fed induction motor drive. The theoretical design, analysis, and experimentation results validate the advantages of the proposed PWM design and its implementation. In addition, the proposed implementation is executed from the MATLAB Xilinx system generator directly into target FPGA, which makes it faithful, efficient and minimizes the time spent.
Radiation in the Optical and UHF Range Emitted by Partial Discharges
Authors: Kozioł Michał , Nagi Łukasz , Kunicki Michał , Urbaniec Ireneusz
Published: November 2019

This paper presents the results of analysis of a solid dielectric’s influence on the phenomena associated with the signals emitted by surface partial discharges. Three types of electrode systems were tested, in which solid dielectrics made of pressboard, Teflon, and glass were used. The emission of such signals as radio waves in the Ultra high frequency (UHF) range and optical radiation was analyzed. The measurements were carried out in the insulation systems most commonly used in electrical power equipment, i.e., mineral oil and air. UHF and optical spectrophotometry methods were used to register the emitted signals. The obtained results indicate that the type of material from which the dielectric solid is made may have a potential impact on some ranges of emitted electromagnetic waves during the surface electrical discharges. The research topic undertaken is important in issues associated with high-voltage insulation systems and in particular with surface discharges, which are often the cause of their damage.
The Age of the ‘Socialist-Wahhabi-Nationalist Revolutionary’: The Fusion of Islamic Fundamentalism and Socialism in Tatar Nationalist Thought, 1898–1917
Authors: Ross Danielle
Published: November 2019

This article examines the relationship among radical socialism, Islamic balanced reform and Tatar national identity in early twentieth-century Russia. In contrast to previous studies, which either have studied these various intellectual strains individually or have positioned Islamic legal and theological reforms as precursors to the emergence of a secular national identity among Kazan’s Tatars, I will argue that Tatar intellectuals’ positions on theology, socio-economic organization, and national identity were mutually reinforcing. Supporters of nationalism also embraced socialism and Islamic balanced reform because they saw all three ideologies as egalitarian and liberating.
On-Site Water and Wind Erosion Experiments Reveal Relative Impact on Total Soil Erosion
Authors: Marzen Miriam , Iserloh Thomas , Fister Wolfgang , Seeger Manuel , Rodrigo-Comino Jesus , Ries Johannes B.
Published: November 2019

The relative impact of water and wind on total erosion was investigated by means of an experimental-empirical study. Wind erosion and water erosion were measured at five different sites: (1) Mediterranean fallow, (2) Mediterranean orchard, (3) wheat field, (4) vineyard and (5) sand substrate. Mean erosion rates ranged from 1.55 to 618 g·m−2·h−1 for wind and from 0.09 to 133.90 g·m−2·h−1 for rain eroded material over all tested sites. Percentages (%) of eroded sediment for wind and rain, respectively, were found to be 2:98 on Mediterranean fallow, 11:89 on Mediterranean orchard, 3:97 on wheat field, 98:2 on vineyard and 99:1 on sand substrate. For the special case of soil surface crust destroyed by goat trampling, the measured values emphasize a strong potential impact of herding on total soil erosion. All sites produced erosion by wind and rain, and relations show that both erosive forces may have an impact on total soil erosion depending on site characteristics. The results indicate a strong need to focus on both wind and water erosion particularly concerning soils and substrates in vulnerable environments. Measured rates show a general potential erosion depending on recent developments of land use and climate change and may raise awareness of scientist, farmers and decision makers about potential impact of both erosive forces. Knowledge about exact relationship is key for an adapted land use management, which has great potential to mitigate degradation processes related to climate change.
Clinical Determinants Affecting Indications for Surgery and Chemotherapy in Recurrent Ovarian Granulosa Cell Tumor
Authors: Nakai Hidekatsu , Koike Eiji , Murakami Kosuke , Takaya Hisamitsu , Kotani Yasushi , Nakai Rika , Suzuki Ayako , Aoki Masato , Matsumura Noriomi , Mandai Masaki
Published: November 2019

Background: Because reports on the management of recurrent granulosa cell tumor have been sparse, a consensus as to which patients should undergo surgical resection and which patients should be considered for chemotherapy has not been established. Methods: A total of 21 tumor recurrences in eight patients with granulosa cell tumor were reviewed. Results: Surgery was performed as the main treatment for 13 recurrences, while chemotherapy was chosen as the main treatment for eight recurrences. Complete tumor resection could be accomplished in 13 of 16 surgeries (81.3%), which include all the ten recurrences without involvement of liver or diaphragm and without ascites. The number of recurrent masses was significantly higher in the early recurrence group (progression free survival < 2 years) than in the late recurrence (progression free survival > 2 years). All cases with a solitary recurrent tumor at an extra-peritoneal site presented a significantly longer progression free survival. Conclusions: For patients with recurrent granulosa cell tumor, surgery may provide the best disease control. In cases with complete resection, the number of recurrent masses was the predictive factor for the next recurrence, and adjuvant chemotherapy might be considered in such cases.
Socioeconomic Inequalities in e-Cigarette Use in Korea: Comparison with Inequalities in Conventional Cigarette Use Using Two National Surveys
Authors: Chang Youngs , Cho Sanghyun , Kim Ikhan , Khang Young-Ho
Published: November 2019

Socioeconomic inequalities in conventional cigarette smoking are well established in developed countries. The aim of this study was to investigate socioeconomic inequalities in e-cigarette use in Korea, in comparison with inequalities in conventional cigarette use. Data from the Korea National Health and Nutrition Examination Survey (KNHANES) and the Korea Community Health Survey (KCHS) were analyzed. The years of data collected were 2013 to 2016 for the KNHANES and 2014 to 2016 for the KCHS, respectively. The age-adjusted prevalence of ever and current e-cigarette use and conventional cigarette use was calculated according to socioeconomic status indicators, including education, occupation, and income. The prevalence of ever e-cigarette use in men increased from 12.1% in 2013 to 19.2% in 2016 in the KNHANES, and from 13.4% in 2014 to 17.9% in 2016 in the KCHS. Ever and current e-cigarette use was concentrated among current smokers and was much more prevalent among men and also more common among younger age groups in men and women. There was higher prevalences of conventional cigarette use among men and women with less education, manual occupational class and lower income, with the differences more pronounced for women. There was higher ever and current use of e-cigarettes among women with less education, manual occupational class and lower income, but among men there was much less difference in e-cigarette use by these indicators of socio-economic status.
Illness Perceptions, Self-Care Management, and Clinical Outcomes According to Age-Group in Korean Hemodialysis Patients
Authors: Kim Sisook , Kim Eunhye , Ryu Eunjung
Published: November 2019

Illness perception, formed in social-cultural contexts, is the driving force for health behavior. Age difference can affect health outcomes due to its association with socioeconomic status. The purpose of this study is to determine the relationship between illness perception, self-care management, and clinical outcomes according to the age group in hemodialysis patients. A cross-sectional study was conducted. Using the Brief Illness Perception Questionnaire (BIPQ) and Patient Activation Measure (PAM) 13, clinical outcomes, such as serum phosphorus, potassium, hemoglobin, and albumin were investigated in maintenance hemodialysis patients. Illness perception, self-care management, and clinical outcomes in 189 hemodialysis patients were different according to age group. Younger hemodialysis patients had the lowest illness perception and the highest serum phosphorus. Illness perception was associated with self-care management and clinical outcomes. After the adjusted age, the association between illness perception and self-care management and the association between illness perception and phosphorus were reduced, while the association between illness perception and potassium increased. Uncontrolled phosphorus in younger hemodialysis patients can increase the risk of cardiovascular complications and mortality. To improve self-care management and clinical outcomes in hemodialysis patients, reconstruction, or expansion of illness perception needs to be differentiated according to age group.
Method for Distinguishing Humans and Animals in Vital Signs Monitoring Using IR-UWB Radar
Authors: Wang Pengfei , Zhang Yang , Ma Yangyang , Liang Fulai , An Qiang , Xue Huijun , Yu Xiao , Lv Hao , Wang Jianqi
Published: November 2019

Radar has been widely applied in many scenarios as a critical remote sensing tool for non-contact vital sign monitoring, particularly for sleep monitoring and heart rate measurement within the home environment. For non-contact monitoring with radar, interference from house pets is an important issue that has been neglected in the past. Many animals have respiratory frequencies similar to those of humans, and they are easily mistaken for human targets in non-contact monitoring, which would trigger a false alarm because of incorrect physiological parameters from the animal. In this study, humans and common pets in families, such as dogs, cats, and rabbits, were detected using an impulse radio ultrawideband (IR-UWB) radar, and the echo signals were analyzed in the time and frequency domains. Subsequently, based on the distinct in-body structure between humans and animals, we propose a parameter, the respiratory and heartbeat energy ratio (RHER), which reflects the contribution rate of breathing and heartbeat in the detected vital signs. Combining this parameter with the energy index, we developed a novel scheme to distinguish between humans and animals. In the developed scheme, after background noise removal and direct-current component suppression, an energy indicator is used to initially identify the target. The signal is then decomposed using a variational mode decomposition algorithm, and the variational intrinsic mode functions that represent human respiration and heartbeat components are obtained and utilized to calculate the RHER parameter. Finally, the RHER index is applied to rapidly distinguish between humans and animals. Our experimental results demonstrate that the proposed approach more effectively distinguishes between humans and animals in terms of monitoring vital signs than the existing methods. Furthermore, its rapidity and need for only minimal calculation resources enable it to meet the needs of real-time monitoring.
Effect of Probiotics Supplementations on Health Status of Athletes
Authors: Sivamaruthi Bhagavathi Sundaram , Kesika Periyanaina , Chaiyasut Chaiyavat
Published: November 2019

Athletes are prone to several health complications, including upper respiratory tract infections, allergies, and gastrointestinal discomforts during practice and after the performance due to the intense exercise, travel, insufficient rest and restricted food consumption. Probiotics are well known as complementary therapeutic and health supplements for several diseases and disorders. Studies suggest that the intervention of probiotics improved the health status of elite athletes, but the results are not consistent in all the studies. The beneficial effect of probiotic supplementation profoundly relies on species or strain, dose, duration, form, and host physiology. The manuscript summarizes the effect of probiotic supplementation on health status of athletes. The literature was collected from PubMed, Scopus, Web of Science, and Google Scholar using the search term “probiotic and athletes”. As per the literature survey, probiotic supplementation improved the intestinal permeability, immune system, intestinal microbiota, inflammatory system, reduced the severity and incidence of respiratory tract infections, and duration of gastrointestinal symptoms. Several studies were conducted on Lactobacillus species and the outcomes were found to be species- or strain-specific. More studies are required to know the detailed mechanism behind the beneficial effect of probiotic intervention in athletes. Further studies are desired on formulation and optimization of probiotic supplements to develop generalized and personalized sports supplements to boost the overall health and enactment of elite athletes.
Aquaculture Feeds Can Be Outlaws for Eutrophication When Hidden in Rice Fields? A Case Study in Qianjiang, China
Authors: Liu Chunhui , Hu Naijuan , Song Weixuan , Chen Qian , Zhu Liqun
Published: November 2019

Water eutrophication caused by agricultural production has become one of the most important factors that impede sustainable rural environmental governance in China. As a result, the Chinese central and local governments want to reduce the use of nitrogen and phosphorus fertilizer and gain socioeconomical profit simultaneously by promoting crayfish and rice integrated system (CRIS) in the rural areas with abundant water resources. In this article, we investigated whether CRIS in Qianjiang, Hubei, the origin place of the system in China, contributes to fulfilling the governments’ expectations. We found that CRIS efficaciously cuts the fertilizer rate in rice production and boosts farmers’ incomes because crayfish has a demand for water quality and holds a large internal market requirement. However, higher profit encourages farmers to expand crayfish production and thus discourages the initiatives in rice production. The area of the ditch for crayfish production expands ceaselessly and exceeds the limit of regulation of CRIS. As a result, the CRIS in the areas has emerged as a practice of aquaculture but in farmland. This is a regulatory gap. The input–output analysis of CRIS by material balance method can also reveal that excessive feed for crayfish has become a new source of agricultural pollution. Beyond that, due to the changed irrigation system and increased water exchange frequency of CRIS, the pollution has transformed from passive distribution to active, which will increase the risk of water eutrophication on a large area.
Association between Use of Nutritional Labeling and the Metabolic Syndrome and Its Components
Authors: Jin Hyung-sub , Choi Eun-bee , Kim Minseo , Oh Sarah Soyeon , Jang Sung-In
Published: November 2019

In this study, we looked into the association between the diagnosis of metabolic syndrome (MetS) and nutritional label awareness. This study used data from the Korea National Health and Nutritional Examination Survey (KNHANES) for the years 2007 to 2015. The study population consisted of a total of 41,667 Koreans of which 11,401 (27.4%) were diagnosed with metabolic syndrome and 30,266 (72.6%) were not. Groups not using nutritional labeling had a 24% increase in odds risk (OR: 1.24, 95% CI 1.14–1.35) of MetS compared to groups using nutritional labeling. Use of nutritional labeling was associated with all components of MetS. Central obesity showed the highest increase in odds risk (OR: 1.23, 95% CI 1.13–1.35) and high blood pressure showed the lowest increase in odds risk (OR: 1.11, 95% CI 1.02–1.20). Subgroup analysis revealed that statistically significant factors were smoking status, drinking status and stress status. Groups that smoke, groups that do not drink and groups with high stress were more vulnerable to MetS when not using nutritional labeling. People not using food labels tends to develop metabolic syndromes more than people using foods labels. In the subgroup analysis, drinking status, smoking status and stress status were significant factors.
Understanding Discomfort in Order to Appropriately Treat Fever
Authors: Doria Mattia , Careddu Domenico , Ceschin Flavia , Libranti Maria , Pierattelli Monica , Perelli Valentina , Laterza Claudia , Chieti Annarita , Chiappini Elena
Published: November 2019

Although national and international guidelines on the management of childhood and adolescent fever are available, some inadequate practices persist, both from parents and healthcare professionals. The main goal of bringing children’s temperature back to normal can lead to the choice of inappropriate drugs or non-necessary combination/alternation of antipyretic treatments. This behavior has been described in the last 35 years with the concept of fever-phobia, caused also by the dissemination of unscientific information and social media. It is therefore increasingly important that pediatricians continue to provide adequate information to parents in order to assess the onset of signs of a possible condition of the child’s discomfort rather than focusing only on temperature. In fact, there is no clear and unambiguous definition of discomfort in literature. Clarifying the extent of the feverish child’s discomfort and the tools that could be used to evaluate it would therefore help recommend that antipyretic treatment is appropriate only if fever is associated with discomfort.
Quadrivalent Influenza Vaccine-Induced Antibody Response and Influencing Determinants in Patients ≥ 55 Years of Age in the 2018/2019 Season
Authors: Ganczak Maria , Dubiel Paulina , Drozd-Dąbrowska Marzena , Hallmann-Szelińska Ewelina , Szymański Karol , Brydak Lidia B.
Published: November 2019

The effects of immunization with subunit inactivated quadrivalent influenza vaccine (QIV) are not generally well assessed in the elderly Polish population. Therefore, this study evaluated vaccine-induced antibody response and its determinants. Methods: Consecutive patients ≥ 55 years old, attending a Primary Care Clinic in Gryfino, Poland, received QIV (A/Michigan/ 45/2015(H1N1)pdm09, A/Singapore/INFIMH-16-0019/2016 (H3N2), B/Colorado/06/2017, B/Phuket/ 3073/2013) between October-December 2018. Hemagglutination inhibition assays measured antibody response to vaccine strains from pre/postvaccination serum samples. Geometric mean titer ratio (GMTR), protection rate (PR) and seroconversion rate (SR) were also calculated. Results: For 108 patients (54.6% males, mean age: 66.7 years) the highest GMTR (61.5-fold) was observed for A/H3N2/, then B/Colorado/06/2017 (10.3-fold), A/H1N1/pdm09 (8.4-fold) and B/Phuket/ 3073/2013 (3.0-fold). Most patients had post-vaccination protection for A/H3N2/ and B/Phuket/3073/ 2013 (64.8% and 70.4%, respectively); lower PRs were observed for A/H1N1/pdm09 (41.8%) and B/Colorado/06/ 2017 (57.4%). The SRs for A/H3N2/, A/H1N1/pdm09, B Victoria and B Yamagata were 64.8%, 38.0%, 46.8%, and 48.2%, respectively. Patients who received QIV vaccination in the previous season presented lower (p < 0.001 and p = 0.03, respectively) response to B Victoria and B Yamagata. Conclusions: QIV was immunogenic against the additional B lineage strain (B Victoria) without significantly compromising the immunogenicity of the other three vaccine strains, therefore, adding a second B lineage strain in QIV could broaden protection against influenza B infection in this age group. As the QIV immunogenicity differed regarding the four antigens, formulation adjustments to increase the antigen concentration of the serotypes that have lower immunogenicity could increase effectiveness. Prior season vaccination was associated with lower antibody response to a new vaccine, although not consistent through the vaccine strains.
Factors Associated with Suicide Attempts and Suicides in the General Population of Andalusia (Spain)
Authors: Mejías-Martín Yolanda , Luna del Castillo Juan de Dios , Rodríguez-Mejías Candela , Martí-García Celia , Valencia-Quintero Juan Pablo , García-Caro María Paz
Published: November 2019

Discrepant results have been published by studies comparing deaths by suicide with attempted suicides. This study aimed to determine factors associated with suicides and attempted suicides in Andalusia (Spain) between 2007 and 2013, comparing sex, age, year, and suicide method between these populations. A retrospective study was conducted of data on deaths by suicide and attempted suicides over a seven-year period, calculating the sex and age rates for each behavior. Adjusted Poisson regression was used to analyze the association with study variables, and incidence rate ratios were estimated. During the seven-year study period, 20,254 attempted suicides and 5202 deaths by suicide were recorded. The prevalence of attempted suicide did not differ between the sexes, whereas the prevalence of deaths by suicide was three-fold higher among males than among females and increased with higher age. The most frequently used method was the same in males and females for suicide attempts, but differed between the sexes for suicides. The combined influence of sex and age was greater in the model for death by suicide than in the model for attempted suicide. The key differentiating factor was the method used, while the finding of greatest concern was the suicide behavior among the elderly. Preventive strategies should take these differences into account.
Examination of the Changes in Calcium Homeostasis in the Delayed Antiarrhythmic Effect of Sodium Nitrite
Authors: Demeter-Haludka Vivien , Kovács Mária , Prorok János , Nagy Norbert , Varró András , Végh Ágnes
Published: November 2019

We have evidence that the intravenous infusion of sodium nitrite (NaNO2) results in an antiarrhythmic effect when given 24 h prior to an ischemia and reperfusion (I/R) insult in anaesthetized dogs. This protection was associated with the reduction of reactive oxygen species resulting from I/R through the attenuation of mitochondrial respiration. Here, we examined whether the changes in calcium, which also contributes to arrhythmia generation, play a role in the NaNO2-induced effect. On the first day, 30 anaesthetized dogs were treated either with saline or NaNO2 (0.2 µmol/kg/min) for 20 min. Some animals were subjected to a 25 min LAD (anterior descending branch of the left coronary artery) occlusion and 2 min reperfusion (I/R = 4; NaNO2-I/R = 6), or the heart was removed 24 h later. We have shown that nitrite prevented the I/R-induced increase in cellular and mitochondrial calcium deposits. During simulated I/R, the amplitude of the calcium transient and the diastolic calcium level were significantly lower in the nitrite-treated hearts and the ERP (effective refractory period) fraction of the action potential was significantly increased. Furthermore, nitrite also enhanced the mitochondrial respiratory response and prevented the MPTPT opening during calcium overload. These results suggest that nitrite can reduce the harmful consequences of calcium overload, perhaps directly by modulating ion channels or indirectly by reducing the mitochondrial ROS (reactive oxygen species) production.
DEK Is a Potential Biomarker Associated with Malignant Phenotype in Gastric Cancer Tissues and Plasma
Authors: Lee Kam-Fai , Tsai Ming-Ming , Tsai Chung-Ying , Huang Chung-Guei , Ou Yu-Hsiang , Hsieh Ching-Chuan , Hsieh Hsi-Lung , Wang Chia-Siu , Lin Kwang-Huei
Published: November 2019

Gastric cancer (GC) is the second most widespread cause of cancer-related mortality worldwide. The discovery of novel biomarkers of oncoproteins can facilitate the development of therapeutic strategies for GC treatment. In this study, we identified novel biomarkers by integrating isobaric tags for relative and absolute quantitation (iTRAQ), a human plasma proteome database, and public Oncomine datasets to search for aberrantly expressed oncogene-associated proteins in GC tissues and plasma. One of the most significantly upregulated biomarkers, DEK, was selected and its expression validated. Our immunohistochemistry (IHC) (n = 92) and quantitative real-time polymerase chain reaction (qRT-PCR) (n = 72) analyses disclosed a marked increase in DEK expression in tumor tissue, compared with paired nontumor mucosa. Importantly, significantly higher preoperative plasma DEK levels were detected in GC patients than in healthy controls via enzyme-linked immunosorbent assay (ELISA). In clinicopathological analysis, higher expression of DEK in both tissue and plasma was significantly associated with advanced stage and poorer survival outcomes of GC patients. Data from receiver operating characteristic (ROC) curve analysis disclosed a better diagnostic accuracy of plasma DEK than carcinoembryonic antigen (CEA), carbohydrate antigen 19.9 (CA 19.9), and C-reactive protein (CRP), highlighting its potential as an effective plasma biomarker for GC. Plasma DEK is also more sensitive in tumor detection than the other three biomarkers. Knockdown of DEK resulted in inhibition of GC cell migration via a mechanism involving modulation of matrix metalloproteinase MMP-2/MMP-9 level and vice versa. Our results collectively support plasma DEK as a useful biomarker for making diagnosis and prognosis of GC patients.
Brain Susceptibility to Methyl Donor Deficiency: From Fetal Programming to Aging Outcome in Rats
Authors: Hassan Ziad , Coelho David , Kokten Tunay , Alberto Jean-Marc , Umoret Rémy , Daval Jean-Luc , Guéant Jean-Louis , Bossenmeyer-Pourié Carine , Pourié Grégory
Published: November 2019

Deficiencies in methyl donors, folate, and vitamin B12 are known to lead to brain function defects. Fetal development is the most studied but data are also available for such an impact in elderly rats. To compare the functional consequences of nutritional deficiency in young versus adult rats, we monitored behavioral outcomes of cerebellum and hippocampus circuits in the offspring of deficient mother rats and in adult rats fed a deficient diet from 2 to 8 months-of-age. We present data showing that the main deleterious consequences are found in young ages compared to adult ones, in terms of movement coordination and learning abilities. Moreover, we obtained sex and age differences in the deleterious effects on these functions and on neuronal layer integrity in growing young rats, while deficient adults presented only slight functional alterations without tissue damage. Actually, the cerebellum and the hippocampus develop and maturate according to different time lap windows and we demonstrate that a switch to a normal diet can only rescue circuits that present a long permissive window of time, such as the cerebellum, whereas the hippocampus does not. Thus, we argue, as others have, for supplements or fortifications given over a longer time than the developmental period.
The Role of Vascular Smooth Muscle Cells in Arterial Remodeling: Focus on Calcification-Related Processes
Authors: Jaminon Armand , Reesink Koen , Kroon Abraham , Schurgers Leon
Published: November 2019

Arterial remodeling refers to the structural and functional changes of the vessel wall that occur in response to disease, injury, or aging. Vascular smooth muscle cells (VSMC) play a pivotal role in regulating the remodeling processes of the vessel wall. Phenotypic switching of VSMC involves oxidative stress-induced extracellular vesicle release, driving calcification processes. The VSMC phenotype is relevant to plaque initiation, development and stability, whereas, in the media, the VSMC phenotype is important in maintaining tissue elasticity, wall stress homeostasis and vessel stiffness. Clinically, assessment of arterial remodeling is a challenge; particularly distinguishing intimal and medial involvement, and their contributions to vessel wall remodeling. The limitations pertain to imaging resolution and sensitivity, so methodological development is focused on improving those. Moreover, the integration of data across the microscopic (i.e., cell-tissue) and macroscopic (i.e., vessel-system) scale for correct interpretation is innately challenging, because of the multiple biophysical and biochemical factors involved. In the present review, we describe the arterial remodeling processes that govern arterial stiffening, atherosclerosis and calcification, with a particular focus on VSMC phenotypic switching. Additionally, we review clinically applicable methodologies to assess arterial remodeling and the latest developments in these, seeking to unravel the ubiquitous corroborator of vascular pathology that calcification appears to be.
Antibacterial Activity and Anti-Quorum Sensing Mediated Phenotype in Response to Essential Oil from Melaleuca bracteata Leaves
Authors: Wang Wenting , Huang Xiaoqin , Yang Huixiang , Niu Xianqian , Li Dongxiang , Yang Chao , Li Liang , Zou Liting , Qiu Ziwen , Wu Shaohua , Li Yongyu
Published: November 2019

The prominent antibacterial and quorum sensing (QS) inhibition activity of aromatic plants can be used as a novel intervention strategy for attenuating bacterial pathogenicity. In the present work, a total of 29 chemical components were identified in the essential oil (EO) of Melaleuca bracteata leaves by gas chromatography-mass spectrometry (GC-MS). The principal component was methyleugenol, followed by methyl trans-cinnamate, with relative contents of 90.46% and 4.25%, respectively. Meanwhile, the antibacterial activity and the QS inhibitory activity of M. bracteata EO were first evaluated here. Antibacterial activity assay and MIC detection against seven pathogens (Dickeya dadantii Onc5, Staphylococcus aureus ATCC25933, Pseudomonas spp., Escherichia coli ATCC25922, Serratia marcescens MG1, Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum ATCC31532) demonstrated that S. aureus ATCC25933 and S. marcescens MG1 had the higher sensitivity to M. bracteata EO, while P. aeruginosa PAO1 displayed the strongest resistance to M. bracteata EO. An anti-QS (anti-quorum sensing) assay revealed that at sub-minimal inhibitory concentrations (sub-MICs), M. bracteata EO strongly interfered with the phenotype, including violacein production, biofilm biomass, and swarming motility, as well as N-hexanoyl-L-homoserine lactone (C6-HSL) production (i.e., a signaling molecule in C. violaceum ATCC31532) of C. violaceum. Detection of C6-HSL indicated that M. bracteata EO was capable of not only inhibiting C6-HSL production in C. violaceum, but also degrading the C6-HSL. Importantly, changes of exogenous C6-HSL production in C. violaceum CV026 revealed a possible interaction between M. bracteata EO and a regulatory protein (cviR). Additionally, quantitative real-time polymerase chain reaction (RT-qPCR) analysis demonstrated that the expression of QS-related genes (cviI, cviR, vioABCDE, hmsNR, lasA-B, pilE1, pilE3, and hcnB) was significantly suppressed. Conclusively, these results indicated that M. bracteata EO can act as a potential antibacterial agent and QS inhibitor (QSI) against pathogens, preventing and controlling bacterial contamination.
miRNAs Regulate Cytokine Secretion Induced by Phosphorylated S100A8/A9 in Neutrophils
Authors: Jung Nicolas , Schenten Véronique , Bueb Jean-Luc , Tolle Fabrice , Bréchard Sabrina
Published: November 2019

The release of cytokines by neutrophils constitutes an essential process in the development of inflammation by recruiting and activating additional cells. Neutrophils are also able to secrete a complex of S100A8 and S100A9 proteins (S100A8/A9), which can amplify the general inflammatory state of the host and is involved in the pathogenesis of several chronic inflammatory diseases, such as rheumatoid arthritis (RA). S100A8/A9 have received renewed attention due to their susceptibility to several function-altering post-translational modifications. In that context, it has been recently demonstrated that only the phosphorylated form of S100A8/A9 (S100A8/A9-P) is able to induce the secretion of several cytokines in neutrophils. Here, we investigate the mechanism by which this post-translational modification of S100A8/A9 can regulate the extracellular activity of the protein complex and its impact on the inflammatory functions of neutrophils. We found that S100A8/A9-P are present in large amounts in the synovial fluids from RA patients, highlighting the importance of this form of S100A8/A9 complex in the inflammation process. Using miRNA-sequencing on S100A8/A9-P-stimulated differentiated HL-60 cells, we identified a dysregulation of miR-146a-5p and miR-155-5p expression through TRL4 signaling pathways. Our data reveal that overexpression of these miRNAs in neutrophil-like cells reduces S100A8/A9-P-mediated secretion of pro-inflammatory cytokines.
Provitamin A Biofortification of Durum Wheat through a TILLING Approach
Authors: Sestili Francesco , Garcia-Molina Maria Dolores , Gambacorta Gianluca , Beleggia Romina , Botticella Ermelinda , De Vita Pasquale , Savatin Daniel Valentin , Masci Stefania , Lafiandra Domenico
Published: November 2019

Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout the world, the biofortification of a major staple crop, such as wheat, represents an effective way to preserve human health in developing countries. In the present work, a key enzyme involved in the branch of carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by a Targeting Induced Local Lesions in Genomes (TILLING) approach in a “block strategy” perspective. The null mutant genotype showed a strong reduction in the expression of the lcyE gene and also interesting pleiotropic effects on an enzyme (β-ring hydroxylase) acting downstream in the pathway. Biochemical profiling of carotenoids in the wheat mutant lines showed an increase of roughly 75% in β-carotene in the grains of the complete mutant line compared with the control. In conclusion, we describe here the production and characterization of a new wheat line biofortified with provitamin A obtained through a nontransgenic approach, which also sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat.
Fast Vibrational Modes and Slow Heterogeneous Dynamics in Polymers and Viscous Liquids
Authors: Puosi Francesco , Tripodo Antonio , Leporini Dino
Published: November 2019

Many systems, including polymers and molecular liquids, when adequately cooled and/or compressed, solidify into a disordered solid, i.e., a glass. The transition is not abrupt, featuring progressive decrease of the microscopic mobility and huge slowing down of the relaxation. A distinctive aspect of glass-forming materials is the microscopic dynamical heterogeneity (DH), i.e., the presence of regions with almost immobile particles coexisting with others where highly mobile ones are located. Following the first compelling evidence of a strong correlation between vibrational dynamics and ultraslow relaxation, we posed the question if the vibrational dynamics encodes predictive information on DH. Here, we review our results, drawn from molecular-dynamics numerical simulation of polymeric and molecular glass-formers, with a special focus on both the breakdown of the Stokes–Einstein relation between diffusion and viscosity, and the size of the regions with correlated displacements.
The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAirTM) and in Human Bronchial Epithelial Cells (BEAS-2B)
Authors: Rossner Pavel , Cervena Tereza , Vojtisek-Lom Michal , Vrbova Kristyna , Ambroz Antonin , Novakova Zuzana , Elzeinova Fatima , Margaryan Hasmik , Beranek Vit , Pechout Martin , Macoun David , Klema Jiri , Rossnerova Andrea , Ciganek Miroslav , Topinka Jan
Published: November 2019

The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAirTM) and in human bronchial epithelial cells (BEAS-2B) grown at the air–liquid interface were compared. The cells were exposed for one or five days to emissions generated by a Euro 5 direct injection spark ignition engine. The general condition of the cells was assessed by the measurement of transepithelial electrical resistance and mucin production. The cytotoxic effects were evaluated by adenylate kinase (AK) and lactate dehydrogenase (LDH) activity. Phosphorylation of histone H2AX was used to detect double-stranded DNA breaks. The expression of the selected 370 relevant genes was analyzed using next-generation sequencing. The exposure had minimal effects on integrity and AK leakage in both cell models. LDH activity and mucin production in BEAS-2B cells significantly increased after longer exposures; DNA breaks were also detected. The exposure affected CYP1A1 and HSPA5 expression in MucilAirTM. There were no effects of this kind observed in BEAS-2B cells; in this system gene expression was rather affected by the time of treatment. The type of cell model was the most important factor modulating gene expression. In summary, the biological effects of complete emissions exposure were weak. In the specific conditions used in this study, the effects observed in BEAS-2B cells were induced by the exposure protocol rather than by emissions and thus this cell line seems to be less suitable for analyses of longer treatment than the 3D model.
Antimicrobial Peptides as Anti-Infective Agents in Pre-Post-Antibiotic Era?
Authors: Rončević Tomislav , Puizina Jasna , Tossi Alessandro
Published: November 2019

Resistance to antibiotics is one of the main current threats to human health and every year multi-drug resistant bacteria are infecting millions of people worldwide, with many dying as a result. Ever since their discovery, some 40 years ago, the antimicrobial peptides (AMPs) of innate defense have been hailed as a potential alternative to conventional antibiotics due to their relatively low potential to elicit resistance. Despite continued effort by both academia and start-ups, currently there are still no antibiotics based on AMPs in use. In this study, we discuss what we know and what we do not know about these agents, and what we need to know to successfully translate discovery to application. Understanding the complex mechanics of action of these peptides is the main prerequisite for identifying and/or designing or redesigning novel molecules with potent biological activity. However, other aspects also need to be well elucidated, i.e., the (bio)synthetic processes, physiological and pathological contexts of their activity, and a quantitative understanding of how physico-chemical properties affect activity. Research groups worldwide are using biological, biophysical, and algorithmic techniques to develop models aimed at designing molecules with the necessary blend of antimicrobial potency and low toxicity. Shedding light on some open questions may contribute toward improving this process.