bluuebunny/crossref_metadata_2025 huggingface dataset:
Interaction of Anabolic Androgenic Steroids and Induced Tooth Movement in Rats
Authors: Karakida Lilian Mary , Araujo Cristiano Miranda de , Johann Aline Cristina Batista Rodrigues , Camargo Elisa Souza , Tanaka Orlando Motohiro , Guariza Filho Odilon Guariza
Published: November 2017

Abstract This study evaluated the interaction between tooth movement and two anabolic androgenic steroids (AAS), Deposteron® and Nebido®. One hundred Wistar rats were divided into 3 groups: control (C) n=30, Nebido experimental (N) n=35 and Deposteron experimental (D) n=35. The control group was subdivided into 6 subgroups: 1, 2, 3, 5, 7 and 14. The experimental groups were subdivided into 7 subgroups: 0, 1, 2, 3, 5, 7 and 14, which corresponded to the day of animal’s euthanasia after applying orthodontic force. Orthodontic devices were used to induce tooth movement using 50 cN of reciprocal force between the maxillary right first molar and the maxillary incisors. After euthanasia, the tissues were processed and stained with hematoxylin and eosin (HE) and tartrate-resistant acid phosphatase (TRAP). Osteoclasts, Howship’s lacunae and blood vessels were quantified. Groups N and D showed acceleration in the reorganization of the periodontal ligament compared to group C. The peak of the histological events occurred in group C on day 5 and in groups N and D on day 3 after installation of the orthodontic device. There was a statistically significant difference in the number of osteoclasts (p<0.05) between groups N3 and C3, and between groups N3 and D3. Supra-physiological doses of the AAS Nebido® and Deposteron® altered the number of osteoclasts, Howship’s lacunae and blood vessels, accelerating the reorganization of the periodontal ligament, resulting in accelerated biological effects from the induced tooth movement in rats.
Quantification of Streptococcus mutans in Different Types of Ligature Wires and Elastomeric Chains
Authors: Shirozaki Mariana Umekita , Ferreira José Tarcísio Lima , Küchler Erika Calvano , Matsumoto Mírian Aiko Nakane , Aires Carolina Patrícia , Nelson-Filho Paulo , Romano Fábio Lourenço
Published: November 2017

Abstract This study aimed to test the hypothesis that Streptococcus mutans contamination levels differ according to the type of the orthodontic ligature. Thirteen patients were selected. Each quadrant was randomly subjected to one of the following ligature-use protocols: I) elastomeric chain, II) steel ligature crossed over the archwire, III) steel ligature crossed under the archwire, and IV) steel ligature in a figure-eight pattern under the archwire. After seven days, the devices were removed and the Streptococcus mutans colony-forming unit count per mg of biofilm weight was determined. Twelve specimens (n=3) were also processed for scanning electron microscopy analysis. ANOVA and Tukey-Kramer test were used for comparisons to assess S. mutans differences between groups at a 5% significance level. There was no statistical difference in detectable levels of S. mutans among the groups (p=0.294). Scanning electron microscopy results showed abundant biofilms and microbial contamination in all groups. In conclusion, S. mutans contamination levels are similar in the different orthodontic ligatures.
Effect of a Bioactive Glass Ceramic on the Control of Enamel and Dentin Erosion Lesions
Authors: Chinelatti Michelle Alexandra , Tirapelli Camila , Corona Silmara Aparecida Milori , Jasinevicius Renato Goulart , Peitl Oscar , Zanotto Edgar Dutra , Pires-de-Souza Fernanda de Carvalho Panzeri
Published: November 2017

Abstract This study evaluated the effect of a bioactive glass ceramic for the control of erosion and caries lesions. Fragments (n=10) of bovine enamel and root dentin received daily application of different treatments (Biosilicate; Acidulated Phosphate Fluoride- APF; Untreated - control) during the performance of erosive cycles. Surfaces were analyzed with 3D optical profilometry to quantify the superficial loss in four periods (1, 7, 14 and 21 days), as well as the lesion depth with confocal laser scanning microscopy. For caries progression assessment, initial Knoop microhardness was measured on enamel bovine fragments. Initial carious lesions were developed and specimens were divided into three groups (n=10), according to the daily topical application (Biosilicate; APF; no application - control), during the de-remineralization cycles for 14 days. Final microhardness was obtained to calculate the change of surface microhardness. Subsurface demineralization was analyzed using cross-sectional microhardness (depths 10, 30, 50, 70, 90, 110 and 220 µm). Data were tested using ANOVA and Tukey’s test (a=5%). Results of erosive evaluation showed that Biosilicate promoted the lowest (p<0.05) values of surface loss, regardless of time, for both enamel and dentin; APF promoted lower (p<0.05) surface loss than control; analyzing different periods of time, APF did not show difference (p>0.05) between 14 and 21 days of demineralization. Results of enamel caries assessment showed that Biosilicate resulted in higher (p<0.05) surface and subsurface microhardness than both APF and control-applications. It may be concluded that Biosilicate application showed a higher potential to reduce surface loss and development of erosion and caries lesions.
Marginal and Internal Adaptation of Zirconia Crowns: A Comparative Study of Assessment Methods
Authors: Cunali Rafael Schlögel , Saab Rafaella Caramori , Correr Gisele Maria , Cunha Leonardo Fernandes da , Ornaghi Bárbara Pick , Ritter André V. , Gonzaga Carla Castiglia
Published: November 2017

Abstract Marginal and internal adaptation is critical for the success of indirect restorations. New imaging systems make it possible to evaluate these parameters with precision and non-destructively. This study evaluated the marginal and internal adaptation of zirconia copings fabricated with two different systems using both silicone replica and microcomputed tomography (micro-CT) assessment methods. A metal master model, representing a preparation for an all-ceramic full crown, was digitally scanned and polycrystalline zirconia copings were fabricated with either Ceramill Zi (Amann-Girrbach) or inCoris Zi (Dentslpy-Sirona), n=10. For each coping, marginal and internal gaps were evaluated by silicone replica and micro-CT assessment methods. Four assessment points of each replica cross-section and micro-CT image were evaluated using imaging software: marginal gap (MG), axial wall (AW), axio-occlusal angle (AO) and mid-occlusal wall (MO). Data were statistically analyzed by factorial ANOVA and Tukey test (a=0.05). There was no statistically significant difference between the methods for MG and AW. For AO, there were significant differences between methods for Amann copings, while for Dentsply-Sirona copings similar values were observed. For MO, both methods presented statistically significant differences. A positive correlation was observed determined by the two assessment methods for MG values. In conclusion, the assessment method influenced the evaluation of marginal and internal adaptation of zirconia copings. Micro-CT showed lower marginal and internal gap values when compared to the silicone replica technique, although the difference was not always statistically significant. Marginal gap and axial wall assessment points showed the lower gap values, regardless of ceramic system and assessment method used.
Cheiloscopic Study among Monozygotic Twins, Non-Twin Brothers and Non-Relative Individuals
Authors: Fernandes Larissa Chaves Cardoso , Oliveira Julyana de Araújo , Santiago Bianca Marques , Rabello Patrícia Moreira , Carvalho Marcus Vitor Diniz de , Campello Reginaldo Inojosa Carneiro , Soriano Evelyne Pessoa
Published: November 2017

Abstract This study aimed to investigate cheiloscopic patterns among monozygotic twins (MT), non-twin siblings (NTS) and unrelated individuals (UI). The sample consisted of 20 pairs of monozygotic twins (G1), 20 pairs of non-twin siblings (G2) and 20 pairs of unrelated individuals (G3). Lip thickness, commissures and grooves were evaluated and the latter were classified as: I - clear-cut vertical grooves; I’ - incomplete vertical grooves; II - branched grooves; III - intersecting; IV - reticular grooves and V - undetermined, in 8 labial regions (sub-quadrants). The data were analyzed using descriptive and inferential statistics, with a 5% significance level. Concordance Correlation Coefficient (CCC) and Kappa coefficient, with a 95% confidence interval, were employed to check for agreement rates between G1, G2 and G3 pairs. There was a weak correlation for lip thickness (<0.90) in the total group, with higher values in G1 (CCC from 0.25 to 0.83 and from 0.34 to 0.86, upper and lower lips, respectively), followed by G2 (CCC from -0.03 to 0.70 and from -0.21 to 0.62, upper and lower lips, respectively) and G3 (CCC from -0.25 to 0.56 and from -0.38 to 0.34, upper and lower lips, respectively). With regard to labial commissures, Kappa statistic values were found to be 1.00 for G1, 0.45 for G2 and -0.24 for G3. As for the main groove pattern by sub-quadrant, Kappa values ranged from 0.48 to 0.87 for G1, from 0.17 to 0.59 for G2, and from -0.18 to 0.19 for G3. Monozygotic twins presented a relevant percentage of cheiloscopic agreements. Similarities were also found among NTS, while UI showed greater differences compared to their pairs, highlighting the influence of hereditary relationships on inherited cheiloscopic features.
Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis
Authors: Dodo Cindy Goes , Meirelles Luiz , Aviles-Reyes Alejandro , Ruiz Karina Gonzalez Silvério , Abranches Jacqueline , Cury Altair Antoninha Del Bel
Published: November 2017

Abstract During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS). THP-1 cell were used and treated for 12, 24 and 48 h following 6 groups: Control(C), PgLPS (L); Microparticles (M); Nanoparticles (N); PgLPS and microparticles (LM); PgLPS and nanoparticles (LN). The following assays were carried out: i) cell viability using MTS, ii) cell morphology by SEM and iii) expression of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by qRT-PCR and ELISA. For statistics two-way ANOVA followed by Tukey’s test was used (p<0.05). After treatment, cells presented similar viability and morphology demonstrating that the treatments were not able to induce cell death. Gene expression was significantly higher for TNF-α and IL1-β after 12 h, and for IL-6 after 24 h in the N and LN groups. Cytokine production over time was an ascending curve for TNF-α with the peak at 48 h and IL1-β and IL-6 had a straight line among the time points, although cells from N group presented a significant production of IL-6 at 48 h. In conclusion, these results suggest that titanium nanoparticles stimulate stronger pro-inflammatory response in macrophages, independent of their association with LPS from P.gingivalis.
Effect of Intermediate Flush Using Different Devices to Prevent Chemical Smear Layer Formation
Authors: Silva Camilla Corrêa , Ferreira Vivian Maria Durange , De-Deus Gustavo , Herrera Daniel Rodrigo , Prado Maíra do , Silva Emmanuel João Nogueira Leal da
Published: November 2017

Abstract This study compared the effect of intermediate flush with distilled water delivered by conventional irrigation, EndoVac microcannula or Self-Adjusting File (SAF) system in the prevention of chemical smear layer (CSL) formation. Thirty human premolars were used. Canals were prepared with Reciproc system and 5.25% NaOCl. After chemomechanical preparation, samples were divided in 3 groups (n=10) according to the intermediate irrigation protocol with distilled water using: conventional irrigation, EndoVac microcannula or SAF. A final flush with 2% chlorhexidine solution was used and scanning electron microscopy was performed to assess protocol effectiveness. Two calibrated evaluators attributed scores according the presence or absence of CSL on the surface of the root canal walls at the coronal, middle and apical thirds, as follows: (1) no CSL; (2) small amounts of CSL; (3) moderate CSL; and (4) heavy CSL. Differences between protocols were analyzed with Kruskal-Wallis and Mann-Whitney U tests. Friedman and Wilcoxon signed rank tests were used for comparison between each root canal third. SAF resulted in less formation of CSL when compared with the conventional irrigation and EndoVac microcannula (p<0.05). When root canal thirds were analyzed, conventional irrigation and EndoVac groups showed less CSL formation at coronal and middle thirds in comparison to the apical third (p<0.05). In SAF group, there was no difference among the thirds (p>0.05). It may be concluded that an intermediate flush of distilled water, delivered by the SAF system resulted in a better reduction of CSL formation during chemomechanical preparation.
Dosimetry in CBCT with Different Protocols: Emphasis on Small FOVs Including Exams for TMJ
Authors: Nascimento Helena Aguiar Ribeiro , Andrade Marcos Ely Almeida , Frazão Marco Antonio Gomes , Nascimento Eduarda Helena Leandro , Ramos-Perez Flavia Maria Moraes , Freitas Deborah Queiroz
Published: November 2017

Abstract This study aimed to estimate the absorbed dose in cone beam computed tomography (CBCT) exams according to different exposure parameters and size and position of the field of view (FOV). In addition was compared the absorbed dose of two smaller FOV scans with that of a larger FOV scan for evaluation of temporomandibular joint (TMJ), as it is a bilateral structure. CBCT scans were obtained on OP300 Maxio unit varying scanning mode (standard, high and endo) as well as size (5x5, 6x8 and 8x15 cm) and positioning of FOV. With a small FOV, different areas were scanned (maxilla or mandible, anterior or posterior and TMJ). Absorbed doses were determined using thermoluminescent dosimeters on the skin surface of sensitive organs of an anthropomorphic phantom. Endo mode showed the highest dose, followed by the high and standard modes in all FOV positions. With small FOV, doses were higher in the posterior region, especially in the mandible. Dose reduction occurred when small FOVs were used, but it was not proportional to FOV size reduction. For TMJ, the dose in a single acquisition with large FOV was greater than two acquisitions with small FOV, but lower than two acquisitions with medium FOV (6x8 cm). In conclusion, scanning mode, size and FOV position have great influence on the absorbed dose. Small FOV decreases the dose, but there is no linear relation between FOV size and dose. For bilateral exams of TMJ, double acquisition with small FOVs produces decrease in absorbed dose relative to a large FOV.
Topographic and Chemical Analysis of Reciprocating and Rotary Instruments Surface after Continuous Use
Authors: Bastos Mariana Mena Barreto , Hanan Aida Rene Assayag , Bastos Ana Mena Barreto , Marques André Augusto Franco , Garcia Lucas da Fonseca Roberti , Sponchiado Júnior Emílio Carlos
Published: November 2017

Abstract This study aimed at evaluating the changes in surface characteristics and NiTi content of reciprocating and rotary instruments after continuous use. Thirty brand-new instruments were assigned to 3 groups (n=10): PNX1 Group - ProTaper Next system - instrument X1; PNX2 Group - ProTaper Next system - instrument X2 and WO Group - WaveOne system - Primary instrument 25.08. The instruments were used to prepare 60 simulated root canals. Analysis of surface characteristics by scanning electron microscopy (SEM) and chemical analysis of Ni and Ti content by energy-dispersive spectroscopy (EDS) were performed before and after the first and third use of the instruments. Only WO Group had significant increase in the quantity of defects and deformations after the third use (p<0.05). PNX1 Group had significant decrease in Ni content after the third use, in comparison with the unused instruments (p<0.05). PNX1 Group had no decrease in Ti content throughout the time of use (p>0.05), however, in PNX2 Group, there was significant decrease in the different time interval of analysis (p<0.05). Continuous use promoted increase in defects and deformations only for WaveOne instruments. Chemical composition presented significant changes according to the time of instruments use.
Microtensile Bond Strength of Adhesive Systems in Different Dentin Regions on a Class II Cavity Configuration
Authors: Sinhoreti Mário Alexandre Coelho , Soares Eveline Freitas , Abuna Gabriel Flores , Correr Sobrinho Lourenço , Roulet Jean-François , Geraldeli Saulo
Published: November 2017

Abstract The aim of this study was to evaluate microtensile bond strength (µTBS) of self-etch and etch-and-rinse adhesives systems compared in different dentin regions (central-CD or proximal-PD) in a class II cavity configuration. A class II (mesial-oclusal-distal) cavity configuration was simulated on 20 extracted human third-molars (4 mm wide/3 mm deep). Etch-and-rinse adhesive (Scotchbond Multi Purpose, n=5, SBMP and Optibond FL, n=5, OPFL) and self-etch adhesives (Clearfil SE Bond, n=5, CSE and Optibond XTR, n=5, OPXTR) were applied. Class II restorations were performed by incremental technique and photo-activated (Bluephase/G2). Samples were sectioned to beam shape (1 mm² cross-section), placed on Geraldeli’s device for µTBS test (0.5 mm/min cross-head speed). Fracture patterns were analyzed on stereomicroscope and classified as cohesive-resin, adhesive, mixed/resin or mixed/dentin. Samples (n=4) were prepared for scanning electron microscope observation. Data were submitted to one-way ANOVA with Split-Plot arrangement and Tukey’s test (α=0.05). There were no statistically significant differences among SBMP, OPFL, CSE and OPXTR on CD (p>0.05). However, on PD for SBMP and OPFL, µTBS values were significantly lower compared to CSE and OPXTR (p<0.05). In all groups, mixed failure pattern was more frequently observed, except for SBMP/CD (adhesive). In class II type cavity configuration, PD location negatively influenced bond strength of etch-and-rinse adhesive systems. Opposite to self-etching adhesives, which presented higher bond strength values compared to etch-and-rinse adhesives in PD.
Fluoride Varnishes against Dental Erosion Caused by Soft Drink Combined with Pediatric Liquid Medicine
Authors: Alexandria Adílis Kalina , Valença Ana Maria Gondim , Cabral Lúcio Mendes , Maia Lucianne Cople
Published: November 2017

Abstract The present study evaluated the effect of NaF and CPP-ACP/NaF varnishes to reduce erosion produced by soft drink (SD) combined or not with pediatric liquid medicine. Enamel specimens were pre-treated with fluoride varnish, according to the following groups: NaF varnish (Duraphat®) or CPP-ACP/NaF varnish (MI varnishTM). Two types of erosive cycles were made: by soft drink erosion (SDE) or by pediatric liquid medicine plus soft drink erosion (PLM/SDE). Bovine enamel specimens were randomly assigned in six groups (n=10): G1=NaF + SDE; G2=CPP-ACP/NaF + SDE; G3=Distilled and deionized (DD) water + SDE; G4=NaF + PLM/SDE; G5=CPP-ACP/NaF + PLM/SDE and G6=DD water + PLM/SDE. Before treatments, the sample surface was divided in two areas (unexposed area-UA and exposed area-EA). The specimens were evaluated by 3D non-contact profilometry technique to determinate tooth structure loss (TSL) and surface roughness (Sa). Scanning electron microscopy (SEM) analysis was also performed. After SDE, G2 presented the lowest TSL values compared to G3 (p=0.008). G1 and G2 did not differ between them (p=0.203) and no groups differed among them despite Sa. Regarding TSL and Sa, G4 and G5 differed from G6 (p=0.0001), but not between them (p=1.00). Examining 3D and SEM images, the greatest differences between UA and EA were observed for G3 and G6. CPP-ACP/NaF varnish seems to be a promising treatment to reduce enamel loss from the erosion produced by a soft drink. Both varnishes also showed capacity to reduce TSL and Sa after erosion by soft drink combined to pediatric liquid medicine.
Inovação por exploração e explotação do conhecimento: um estudo empírico do setor automobilístico
Authors: Gonzalez Rodrigo Valio Dominguez , Melo Tatiana Massaroli de
Published: November 2017

Resumo Diante de ambiente dinâmico, como é o caso do setor automobilístico, alvo deste estudo, as organizações são desafiadas a estabelecer flexibilidade estratégica, conquistada por meio de inovações, que exigem a exploração e explotação do conhecimento. Estudos prévios apontam que a combinação de exploração e explotação do conhecimento é a estratégia ideal para inovação, pois a organização acessará novas tecnologias por meio da exploração de novos conhecimentos e refinará e aperfeiçoará a tecnologia dominante por meio da explotação do conhecimento primário. Grande parte dos estudos sobre o tema foca suas contribuições em analisar o impacto das iniciativas de gestão do conhecimento e da exploração e explotação do conhecimento sobre o desempenho inovativo e financeiro da firma; entretanto, percebem-se poucos estudos que investigam os fatores organizacionais que sustentam os processos de exploração e explotação do conhecimento. Os resultados da pesquisa mostram que os processos de exploração e explotação do conhecimento são impactados de forma distinta por cinco fatores contextuais, considerados nesta pesquisa: gestão de recursos humanos, liderança colaborativa, cultura de aprendizagem, autonomia e sistemas de tecnologia de informação (TI). Enquanto exploração é mais impactada por cultura de aprendizagem, autonomia e sistemas de TI, explotação está mais relacionada com liderança colaborativa e cultura de aprendizagem.
VOLUME AND TAPER EQUATIONS FOR COMMERCIAL STEMS OF Nothofagus obliqua AND N. alpina
Authors: Beltran Hernan Attis , Chauchard Luis , Iaconis Ariana , Pastur Guillermo Martinez
Published: November 2017

ABSTRACT Timber volume of standing trees is essential information for management decisions. The increasing need to optimize the potential capacity of forests maintaining their conservation, requires the quantification of the different potential possible timber products. The aim was to adjust taper equations to determine volumes of different timber products for commercial stems of Nothofagus alpina and N. obliqua. Trees of both species were randomly selected in harvesting areas of Lanin National Park (Argentina). Trees were felled and cut into commercial logs, measuring diameter with bark at different heights up to the beginning of the crown, and for each tree the diameter at breast height and total height. Five taper equations were selected and non-linear regression processes were employed for the fittings. We obtained the volume through the integration of the stem profile equation and the rotation in the space thereof through solid of revolution. The Bennet and Swindel (1972) model was selected for both Nothofagus species, obtaining similar equation parameters and differences were observed at the top of the stems of larger trees. For this the use of an integrated model is not recommended. With the obtained equations it is possible to: (i) estimate volume at different heights and for different commercial diameters, and (ii) predict the height at which both species reach to a certain diameter. The model presented some statistical limitations (e.g. multicollinearity), however, the fitting of the equation and the easy understanding of the outputs support it as a useful tool in a broad range of forest applications.
NIR SPECTROSCOPIC MODELS FOR PHENOTYPING WOOD TRAITS IN BREEDING PROGRAMS OF Eucalyptus benthamii
Authors: Estopa Regiane Abjaud , Milagres Flaviana Reis , Oliveira Ricardo Augusto , Hein Paulo Ricardo Gherardi
Published: November 2017

ABSTRACT Wood characterization must be done in huge populations of Eucalyptus breeding programs in order to efficiently select potential trees. In this study, Eucalyptus benthamii wood was non-destructively characterized and the performance of near infrared (NIR) spectroscopy in estimating the wood basic density, lignin, extractive, glucose, xylan contents and total carbohydrates was evaluated. NIR models for wood traits were performed from 481 trees from E. benthamii progeny test (4-year-old) managed for pulp cultivated in Santa Catarina state, Southern Brazil. Increment cores were sampled for chemical and physical characterization in laboratory, as well as for NIR spectroscopy analyses. Three 350 samples were selected from PCA for model calibrations whereas 131 were reserved for independent test validation. The E. benthamii wood presented the standards required for Kraft pulp processing. The predictive NIR models showed satisfactory ability for estimating the chemical properties of wood. The prediction models for total lignin, extractive and xylan contents and total carbohydrates showed coefficients of determination of 0.53, 0.65; 0.36 and 0.53, with RPD values for these traits ranging from 1.3 to 2.3. The predictive model for basic density of wood and glucose presented low coefficient of determination (0.13 and 0.10). However, isn’t possible to use these models for ranking in genetic selection because there was no correlation. Therefore, NIR spectroscopy can potentially be applied in breeding programs, as it enables an early, non-destructive selection of trees with adequate physical and chemical properties for pulp production process.
GENETIC VARIATION FOR GROWTH VARIABLES OF Eucalyptus benthamii MAIDEN & CAMBAGE AND E. smithii R. T. BAKER PROVENANCES IN SOUTHERN BRAZIL
Authors: Konzen Enéas Ricardo , Navroski Marcio Carlos , Pereira Mariane de Oliveira , Nascimento Bruno , Meneguzzi Aline , Souza Patrícia Fukushima de
Published: November 2017

ABSTRACT Southern Brazil encompasses areas with a subtropical climate, with significant number of frosts, which challenge or even impair growing tropical eucalypts. Eucalyptus benthamii and E. smithii rise as alternative species of subtropical origin with considerable frost-tolerance. Here we accessed the genetic variation for diameter at breast height (DBH), height (H), volume (VOL), bifurcation and straightness of four provenances of E. smithii and two of E. benthamii, cultivated in randomized block design in a farm in Rio Negrinho, Santa Catarina, southern Brazil. We found considerable genetic variability for the traits, all directly associated with the selection for wood production and quality. In general, most of the genetic variability was found within the provenances (Q d ranged from 63.6 to 73.1%), except for straightness (Q d = 13.5% and Q st = 81.9%). Although predicting genetic gains with provenance trials might have some drawbacks, the application of the REML/BLUP method was able to predict genetic gains for the growth variables DBH, H and VOL for provenances of the two species. The highest genetic gains were predicted for a provenance of E. smithii originated from Australia (13% for DBH, 5% for H and 6.1% for VOL). However, the genetic gains from a E. benthamii provenance from Santa Catarina state were not negligible (1.2% for DBH, 1.8 for H and 2.2% for VOL). Our results provide insights for further progeny trials aimed at investigating additive genetic variance and their components, which could improve the genetic control of the traits and assist breeding programs of E. smithii and E. benthamii.
Assessment of serotonergic system in formation of memory and learning
Authors: Silva J. C. da , Amorim C. A. M. , Rodrigues G. P. , Dal Pai J. , Zambrano L. I. , Trindade Filho E. M.
Published: November 2017

Abstract We evaluated the involvement of the serotonergic system on memory formation and learning processes in healthy adults Wistar rats. Fifty-seven rats of 5 groups had one serotonergic nuclei damaged by an electric current. Electrolytic lesion was carried out using a continuous current of 2mA during two seconds by stereotactic surgery. Animals were submitted to learning and memory tests. Rats presented different responses in the memory tests depending on the serotonergic nucleus involved. Both explicit and implicit memory may be affected after lesion although some groups showed significant difference and others did not. A damage in the serotonergic nucleus was able to cause impairment in the memory of Wistar. The formation of implicit and explicit memory is impaired after injury in some serotonergic nuclei.
Cadmium tolerance of Typha domingensis Pers. (Typhaceae) as related to growth and leaf morphophysiology
Authors: Oliveira J. P. V. , Pereira M. P. , Duarte V. P. , Corrêa F. F. , Castro E. M. , Pereira F. J.
Published: November 2017

Abstract Typha domingensis (cattail) is a native macrophyte known by its capacity to tolerate several heavy metals effects and the potential use for phytoremediation. However, in despite that cadmium (Cd) is one of the most toxic pollutants; its effects in T. domingensis biology remain uninvestigated. Thus, the objective of this study was to study the tolerance of T. domingensis to cadmium contamination by evaluating its growth, Cd uptake, leaf anatomy and gas exchange. The experiment was designed using three cadmium concentrations (0, 10 and 50 µM) and ten replicates for 90 days. The cadmium uptake, growth, gas exchange, chlorophyll content and leaf anatomy were evaluated. Data was submitted to ANOVA and Scott-Knott test for P<0.05. Typha domingensis accumulates Cd proportionally to its concentration on the solution and the content of this metal was higher in roots as compared to shoots. Plants showed no significant modifications on growth parameters such as the biomass production, number of leaves, number of clones and the biomass allocation to organs. The photosynthesis, transpiration and chlorophyll content were not modified by Cd. Most anatomical traits evaluated were not modified by the metal but the stomatal density and the proportion of vascular tissues were reduced under 50 µM of Cd. In despite, the leaf anatomy showed no toxicity evidences for any Cd level. The absence of growth reduction and the stability of anatomical and physiological traits give insight about the Cd tolerance of this species. Therefore, T. domingensis is able to overcome Cd toxicity and shows potential for phytoremediation.
Metazoan parasites of the lambari Astyanax altiparanae collected in the Batalha River, State of São Paulo, Brazil
Authors: Negrelli D. C. , Abdallah V. D. , Azevedo R. K.
Published: November 2017

Abstract Here we conduct a qualitative and quantitative analysis of ectoparasites and endoparasites of Astyanax altiparanae (Garutti & Britski, 2000), popularly known as lambari-do-rabo-amarelo collected from the Batalha River, State of São Paulo, Brazil. Of the 67 specimens of A. altiparanae, 52 were parasitized by at least one parasitic metazoan species. The following metazoan parasites were found: Monogenea, Digenea and Nematoda. The monogenetic Diaphorocleidus sp. was the predominant species, presenting a higher frequency of dominance, prevalence and mean abundance. However, the monogenean Trinigyrus sp. was the one with the highest mean intensity value. All parasites showed aggregate distribution pattern. The parasitic community of A. altiparanae was characterized by low diversity, low richness and low uniformity. The digenetic Rhipidocotyle santanaensis is recorded for the first time in A. altiparanae, in the same way, this parasite is being registered for the first time in the Batalha River.
Influence of physical attributes and pedotransfer function for predicting water retention in management systems
Authors: Auler André C. , Pires Luiz F. , Pineda Maria C.
Published: November 2017

ABSTRACT The aims of this study were to evaluate the effects of the soil structural physical attributes on the water retention and to develop pedotransfer functions (PTFs) for the estimation of the soil water content (θ) at different matric potentials of a Dystrophic Red Latosol (Hapludox) under conventional tillage (CT) and no-tillage (NT) soil management systems. The effects of long-term CT and NT (over 25 years) management on the soil bulk density (BD), total porosity (TP), macroporosity (Ma), microporosity (Mi) and water retention were investigated. The PTFs were developed to predict θ and used to evaluate the soil water retention curve only for the NT system. The NT system was characterized by smaller values of the soil BD and higher values of the soil TP and Mi than the CT system. The NT system exhibited a higher θ retained than the CT system for the pore-size interval of 0.2–30 μm. However, the CT system exhibited a large amount of water retention for pores smaller than 0.2 μm. The PTFs were utilized to estimate θ at matric potentials of -1, -3, -6, -10, -33, -100, -400, -800, and -1,500 kPa with adequate accuracy. The soil BD, Ma, Mi and sand content were the main variables considered to estimate θ for the different matric potentials evaluated.
Erosive rainfall in Rio do Peixe Valley in Santa Catarina, Brazil: Part I - Determination of the erosivity index
Authors: Back Álvaro J. , Pola Augusto C. , Ladwig Nilzo I. , Schwalm Hugo
Published: November 2017

ABSTRACT This study aimed to determine the rainfall erosivity index in the Valley of Rio do Peixe, in the state of Santa Catarina, Brazil. The data series of three rain gauge stations in the cities of Campos Novos, Videira, and Caçador were used to determine the rainfall erosivity based on the EI30 index and to adjust the equations in order to estimate the EI30 value from the rainfall coefficient. On average, it was observed that erosive rains represents 81.4-88.5% of the annual precipitation. The adjusted equations can be used to estimate rainfall erosivity in locations with only rainfall data. The regional equation specified for the erosivity estimation is EI30 = 74.23 Rc0.8087. The R factor is 8,704.8; 7,340.8; and 6,387.1 MJ mm ha-1 h-1 year-1 for Campos Novos, Videira, and Caçador, respectively. In Campos Novos and Videira, the erosivity was classified as high, while in Caçador, it was classified as average.
Hydrochemistry of alluvial aquifer in the Cobra River sub-basin
Authors: Lima Alexandre de O. , Dias Nildo da S. , Lima Filho Francisco P. , Ferreira Neto Miguel , Rego Priscila R. do A. , Souza Anderson de M.
Published: November 2017

ABSTRACT The regional characteristics of waters from alluvial aquifers are different, since they depend on geology and climate. Also, there may be local variation in the quality of the available water when the water source is superficial (rivers and lakes) or is underground due to geology. In order to investigate the groundwater quality of the alluvial aquifer in the Sub-Basin of the Cobra River (RN) for construction of underground dams, five wells, four in the main river and one in the tributary, were monitored in dry and rainy seasons, during two hydrological years. Fifteen water samples from ‘Amazonas’ wells were collected for hydrochemical assessment. Multivariate analysis were performed to evaluate the origin of aquifer recharge and water hydrochemical characteristics. Hydrochemical assessment results indicated that Na+ and Cl- ions have greater influence on the salinization of the studied area. There are risks of worsening salinization and toxicity problems in the middle and lower courses of the Cobra River Sub-Basin.
Quality of second-crop corn according to the period between harvest and drying
Authors: Andrade José C. de , Goneli André L. D. , Hartmann Filho Cesar P. , Azambuja Thalita M. S. de , Barboza Valdenise C.
Published: November 2017

ABSTRACT The objective of this study was to evaluate the quality of second-crop corn harvested with different moisture contents as a function of time before drying. The corn grains were harvested with moisture content of 28.5, 22.4, 21 and 19%, and submitted to a temporary storage for ten days, simulating the time between harvesting and drying. Quality was subsequently evaluated every two days, based on the commercial classification of the grains, sanity test and dry bulk density. The results showed that: the increase in moisture content at harvest affects the physical and sanitary quality of second-crop corn, and this effect is aggravated over time; the moisture content of 19% is the one that least affects grain quality during the ten days of temporary storage.
Low-cost automatic station for compost temperature monitoring
Authors: Jordão Marcelo D. L. , Paiva Kary de , Firmo Heloisa T. , Inácio Caio T. , Rotunno Filho Otto C. , Lima Tomé de A. e
Published: November 2017

ABSTRACT Temperature monitoring is an important procedure to control the composting process. Due to cost limitation, temperature monitoring is manual and with daily sampling resolution. The objective of this study was to develop an automatic station with US$ 150 dollars, able to monitor air temperature at two different points in a compost pile, with a 5-min time resolution. In the calibration test, the sensors showed an estimated uncertainty from ± 1 to ± 1.9 ºC. In the field validation test, the station guaranteed secure autonomy for seven days and endured high humidity and extreme temperature (> 70 °C).
Efeito da adição de proteína do soro do leite como substituto do trigo na formulação de bolos sem adição de açúcar
Authors: Soares Jéssica Pazzaro , Marques Gleicyane de Almeida , Magalhães Camila Soares de , Santos Aline Bravim , José Jackline Freitas Brilhante de São , Silva Daniela Alves , Silva Erika Madeira Moreira da
Published: November 2017

Resumo A proteína do soro do leite (PSL) é um produto da fabricação de queijos, que apresenta elevado valor nutricional e atributos funcionais. Sua utilização em produtos de panificação favorece aspectos físicos, como a emulsão e a capacidade de estabilização, além de melhorar características sensoriais. O presente estudo teve como objetivo elaborar bolos sem adição de açúcar, utilizando-se a PSL como substituto do trigo, bem como avaliar suas características físicas, químicas, sensoriais e microbiológicas. O efeito da adição de PSL e margarina em bolos foi avaliado por meio de um delineamento central composto rotacional de segunda ordem. Os tratamentos selecionados foram avaliados por meio de análise sensorial e caracterizados quanto à composição centesimal, além de se proceder à avaliação microbiológica após 15 dias de armazenamento. A adição de até 11,98 g/100 g de margarina influenciou de forma expressiva na redução da altura dos bolos. O tratamento com teor intermediário de PSL (30 g/100 g) e menor teor de margarina (6,72 g/100 g) obteve melhor aceitabilidade e, quando comparado ao controle, maior conteúdo de proteínas. As condições microbiológicas foram satisfatórias, atendendo à legislação vigente, mesmo após 15 dias de armazenamento.
Salad dressing spoilage by Bacillus amyloliquefaciens with gas formation
Authors: Pereira Karen Signori , Cayres Caroline Alves , Chaves Jeane Quintanilha , Brito Josiane Teixeira de , Rabinovitch Leon , Vivoni Adriana Marcos
Published: November 2017

Abstract B. amyloliquefaciens is a Gram-positive, aerobic, motile rod, often found in soil, which has been described as a plant growth promoter and is used in several industrial processes. This study reports an episode involving the gassy spoilage of salad dressing caused by B. amyloliquefaciens in a production facility located in Rio de Janeiro, Brazil. Nine B. amyloliquefaciens strains were isolated from spoiled salad dressings, the sugar used as a raw material in the manufacture and from the production plant. A genotypic analysis of the isolates by Rep-PCR generated eight band profiles grouped in five Rep-PCR clusters. When re-inoculated into fresh salad dressing three B. amyloliquefaciens isolates belonging to the Rep-PCR clusters A, D and E were able to reproduce the gassy spoilage process, whereas the isolates belonging to the Rep-PCR clusters B and C did not produce any visible spoilage, suggesting that these isolates were not directly involved in the spoilage process. The predominant Rep-PCR cluster, cluster A, included strains isolated from barbecue and passion fruit seed salad dressings and from sugar (raw material), suggesting it is a common source of contamination for such salad dressings.
Antiproliferation effects and antioxidant activity of two new Lactobacillus strains
Authors: Shokryazdan Parisa , Jahromi Mohammad Faseleh , Bashokouh Fatemeh , Idrus Zulkifli , Liang Juan Boo
Published: November 2017

Abstract The microorganisms most commonly used as probiotics are lactic acid bacteria, especially those of the genus Lactobacillus. In the present study, two Lactobacillus strains, L. pentosus ITA23 and L. acidipiscis ITA44, previously isolated from mulberry silage, were characterized for their antiproliferative and antioxidant activities. The antiproliferative effects of the strains were investigated using the MTT assay with breast cancer (MDA-MB-231), liver cancer (HepG2) and normal liver (Chang) cell lines. The strains were tested for their antioxidant activity using the FRAP and ABTS methods. The results showed that the two Lactobacillus strains had good antiproliferative effects against both cancer cell lines tested, while their effects on the normal cells were weak. Based on the results of the antioxidant tests, the intact cells and cell-free extracts of the two Lactobacillus strains showed more than 135 and less than 50 µg trolox/ml of antioxidant activity, respectively. Lactobacillus pentosus ITA23 and L. acidipiscis ITA44 can be considered as potential probiotic candidates for humans because of their antioxidant activity and antiproliferation effects against cancer cells.
Influence of process conditions on the physicochemical properties of jussara pulp (Euterpe edulis) powder produced by spray drying
Authors: Santana Audirene Amorim , Paixão Louryval Coelho , Oliveira Rafael Augustus de , Telis Vânia Regina Nicoletti
Published: November 2017

Abstract The objective of this work was to optimize the spray drying of jussara pulp using mixtures of modified starch (MS) with whey protein concentrate (WPC) or soy protein isolate (SPI) as the carrier agents. Two central composite rotatable designs were used to evaluate the effect of the independent variables of inlet air temperature (140 °C to 200 °C), carrier agent concentration - CAC (0.5 to 2 g carrier agent/g jussara pulp solids) and the proportions of MS:WPC or MS:SPI (5 to 30 g WPC or SPI/100 g carrier agent) on the following responses for powders formulated with MS:WPC and MS:SPI, respectively: moisture content (0.3% to 1.4% and 0.6% to 1.2%), solubility (78.0% to 92.9% and 78.9% to 83.8%), retention of total anthocyanins (49.2% to 82.9% and 34.1% to 96.9%), encapsulation efficiency (98.5% to 99.7% and 98.5% to 99.5%), hue angle (9.1 to 44.0 and 3.7 to 42.6), chroma (10.0 to 15.3 and 9.2 to 14.3) and process yield (33.2% to 55.5% and 49.9% to 78.5%). The inlet air temperature 170 °C, CAC of 1.25 and 2 g/g jussara pulp solids and proportion of MS:WPC or MS:SPI of 17.5 and 30 g/100 g were recommended as the selected conditions.
ESPIROMETRIA EM ESCOLARES DURANTE ESTUDOS DE CAMPO: REALIZAÇÃO EM DIAS DIFERENTES ALTERA O RESULTADO DO EXAME?
Authors: Scalco Janaina Cristina , Minsky Rafaela Coelho , Schivinski Camila Isabel Santos
Published: November 2017

RESUMO Objetivo: Analisar se há alteração no resultado do exame espirométrico executado por escolares saudáveis, quando realizado em dias diferentes. Métodos: Estudo transversal com escolares saudáveis com 7 a 12 anos, provenientes de escolas da Grande Florianópolis, Santa Catarina, Brasil. Foram incluídas crianças pré-púberes, não atletas, nascidas a termo, sem qualquer doença cardiorrespiratória, reumática, musculoesquelética, neurológica e déficits visuais ou auditivos. A higidez da criança foi avaliada por meio de um questionário de saúde e do questionário International Study of Asthma and Allergies in Childhood. O exame espirométrico foi realizado em 3 dias diferentes, não excedendo o intervalo de 15 dias entre o primeiro e o último exame, seguindo as diretrizes da American Thoracic Society. Foram analisados os valores absolutos e os percentuais dos valores preditos de capacidade vital forçada, volume forçado no primeiro segundo e pico de fluxo expiratório. A análise estatística foi realizada com o teste de normalidade de Shapiro-Wilk, seguida por análise de variância de uma via ou teste de Friedman e pelo teste post-hoc de Bonferroni para comparações múltiplas paramétricas. Foi aplicado o coeficiente de correlação intraclasse (ICC) para comparar os resultados dos mesmos pacientes entre os diferentes dias. Resultados: Vinte e duas crianças foram analisadas, com média de idade de 9,3±1,1 anos. Todos os parâmetros espirométricos apresentaram redução numérica no decorrer dos dias avaliados, no entanto, sem diferença significante. Na análise da reprodutibilidade da espirometria, os testes apresentaram coeficiente de correlação intraclasse >0,70. Conclusões: Não houve alteração do resultado da espirometria executada por escolares saudáveis em três dias distintos.
Anxiety and depression symptoms in Brazilian sexual minority ecstasy and LSD users
Authors: Remy Lysa S. , Scherer Juliana , Guimarães Luciano , Surratt Hilary L. , Kurtz Steven P. , Pechansky Flavio , Kessler Felix
Published: November 2017

Abstract Background This study examined drug use patterns and psychiatric symptoms of anxiety and depression among young Brazilian sexual minority ecstasy and LSD users and compared findings with those reported for their heterosexual peers. Method This cross-sectional study employed targeted sampling and ethnographic mapping approaches via face-to-face interviews conducted at bars and electronic music festivals using an adapted, semi-structured version of the Global Appraisal of Individual Needs questionnaire. The sample comprised 240 male and female young adults who had used ecstasy and/or LSD in the 90 days prior to the interview and who were not on treatment for alcohol and drug abuse. Results Of the 240 subjects enrolled (mean age: 22.9±4.5 years), 28.7% were gay or bisexuals. Multivariate regression analysis showed that the prevalence of depression symptoms in the past 12 months in the sexual minority group was 37% higher than among heterosexuals (prevalence ratio [PR]=1.79; 95% confidence interval [95%CI] 1.03-3.11; p=0.037). Conclusion Strategies should be developed to assess and address individual needs and treatment approaches should be tailored to address depressive symptoms in young, sexual minority club drug users.
O ensino da tabuada no contexto das ações de estudo propostas por Davýdov e colaboradores
Authors: HOBOLD EDISÉIA SUETHE FAUST , ROSA JOSÉLIA EUZÉBIO DA
Published: November 2017

RESUMO Esta investigação foi desenvolvida no contexto da educação matemática, com base nos pressupostos da teoria histórico-cultural, com ênfase na obra de Davýdov, doutor em psicologia e seguidor de Vigotski. Davýdov coordenou o processo de elaboração de uma proposta para o ensino de matemática na Rússia e efetivou sua publicação por meio de livros didáticos e manuais de orientação ao professor. Esse material é a fonte de dados da pesquisa que consistem nas tarefas davydovianas e cujo objetivo se constitui em investigar o movimento conceitual impresso na proposição davydoviana para o ensino de tabuada por meio das ações de estudo. Os resultados indicam que a proposição do autor, para o ensino da tabuada, contempla a inter-relação das significações aritméticas, algébricas e geométricas no movimento que envolve o geral, universal, particular e singular no contexto de ações de estudo.