bluuebunny/crossref_metadata_2025 huggingface dataset:
The Hepatic Mitochondrial Pyruvate Carrier as a Regulator of Systemic Metabolism and a Therapeutic Target for Treating Metabolic Disease
Authors: McCommis Kyle S. , Finck Brian N.
Published: January 2023

Pyruvate sits at an important metabolic crossroads of intermediary metabolism. As a product of glycolysis in the cytosol, it must be transported into the mitochondrial matrix for the energy stored in this nutrient to be fully harnessed to generate ATP or to become the building block of new biomolecules. Given the requirement for mitochondrial import, it is not surprising that the mitochondrial pyruvate carrier (MPC) has emerged as a target for therapeutic intervention in a variety of diseases characterized by altered mitochondrial and intermediary metabolism. In this review, we focus on the role of the MPC and related metabolic pathways in the liver in regulating hepatic and systemic energy metabolism and summarize the current state of targeting this pathway to treat diseases of the liver. Available evidence suggests that inhibiting the MPC in hepatocytes and other cells of the liver produces a variety of beneficial effects for treating type 2 diabetes and nonalcoholic steatohepatitis. We also highlight areas where our understanding is incomplete regarding the pleiotropic effects of MPC inhibition.
Lnc-uc.147 Is Associated with Disease Stage of Liver, Gastric, and Renal Cancer
Authors: Rodrigues Ana Carolina , Zambalde Erika Pereira , Bellan Daniel de Lima , Trindade Edvaldo da Silva , Ribeiro Enilze Maria de Souza Fonseca , Calin George , Gradia Daniela Fiori , Carvalho de Oliveira Jaqueline
Published: January 2023

Lnc-uc.147, a long non-coding RNA derived from a transcribed ultraconserved region (T-UCR), was previously evidenced in breast cancer. However, the role of this region in other tumor types was not previously investigated. The present study aimed to investigate lnc-uc.147 in different types of cancer, as well as to suggest lnc-uc.147 functional and regulation aspects. From solid tumor datasets analysis of The Cancer Genome Atlas (TCGA), deregulated lnc-uc.147 expression was associated with the histologic grade of hepatocellular carcinoma, and with the tumor stage of clear cell renal and gastric adenocarcinoma. Considering the epidemiologic relevance of liver cancer, silencing lnc-uc.147 reduced the viability and clonogenic capacity of HepG2 cell lines. Additionally, we suggest a relation between the transcription factor TEAD4 and lnc-uc.147 in liver and breast cancer cells.
The Effect of Tortuosity on Permeability of Porous Scaffold
Authors: Prakoso Akbar Teguh , Basri Hasan , Adanta Dendy , Yani Irsyadi , Ammarullah Muhammad Imam , Akbar Imam , Ghazali Farah Amira , Syahrom Ardiyansyah , Kamarul Tunku
Published: February 2023

In designing porous scaffolds, permeability is essential to consider as a function of cell migration and bone tissue regeneration. Good permeability has been achieved by mimicking the complexity of natural cancellous bone. In this study, a porous scaffold was developed according to the morphological indices of cancellous bone (porosity, specific surface area, thickness, and tortuosity). The computational fluid dynamics method analyzes the fluid flow through the scaffold. The permeability values of natural cancellous bone and three types of scaffolds (cubic, octahedron pillar, and Schoen’s gyroid) were compared. The results showed that the permeability of the Negative Schwarz Primitive (NSP) scaffold model was similar to that of natural cancellous bone, which was in the range of 2.0 × 10−11 m2 to 4.0 × 10−10 m2. In addition, it was observed that the tortuosity parameter significantly affected the scaffold’s permeability and shear stress values. The tortuosity value of the NSP scaffold was in the range of 1.5–2.8. Therefore, tortuosity can be manipulated by changing the curvature of the surface scaffold radius to obtain a superior bone tissue engineering construction supporting cell migration and tissue regeneration. This parameter should be considered when making new scaffolds, such as our NSP. Such efforts will produce a scaffold architecturally and functionally close to the natural cancellous bone, as demonstrated in this study.
Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia
Authors: Viasus Diego , Simonetti Antonella F. , Nonell Lara , Vidal Oscar , Meije Yolanda , Ortega Lucía , Arnal Magdalena , Bódalo-Torruella Marta , Sierra Montserrat , Rombauts Alexander , Abelenda-Alonso Gabriela , Blanchart Gemma , Gudiol Carlota , Carratalà Jordi
Published: February 2023

(1) Background: Information regarding gene expression profiles and the prognosis of community-acquired pneumonia (CAP) is scarce. We aimed to examine the differences in the gene expression profiles in peripheral blood at hospital admission between patients with CAP who died during hospitalization and those who survived. (2) Methods: This is a multicenter study of nonimmunosuppressed adult patients who required hospitalization for CAP. Whole blood samples were obtained within 24 h of admission for genome-expression-profile analysis. Gene expression profiling identified both differentially expressed genes and enriched gene sets. (3) Results: A total of 198 samples from adult patients who required hospitalization for CAP were processed, of which 13 were from patients who died. Comparison of gene expression between patients who died and those who survived yielded 49 differentially expressed genes, 36 of which were upregulated and 13 downregulated. Gene set enrichment analysis (GSEA) identified four positively enriched gene sets in survivors, mainly associated with the interferon-alpha response, apoptosis, and sex hormone pathways. Similarly, GSEA identified seven positively enriched gene sets, associated with the oxidative stress, endoplasmic reticulum stress, oxidative phosphorylation, and angiogenesis pathways, in the patients who died. Protein–protein-interaction-network analysis identified FOS, CDC42, SLC26A10, EIF4G2, CCND3, ASXL1, UBE2S, and AURKA as the main gene hubs. (4) Conclusions: We found differences in gene expression profiles at hospital admission between CAP patients who died and those who survived. Our findings may help to identify novel candidate pathways and targets for potential intervention and biomarkers for risk stratification.
Effect of the Enrichment in c-Kit Stem Cell Potential of Foetal Human Amniotic Fluid Cells: Characterization from Single Cell Analysis to the Secretome Content
Authors: Casciaro Francesca , Beretti Francesca , Gatti Martina , Persico Giuseppe , Bertucci Emma , Giorgio Marco , Maraldi Tullia
Published: February 2023

Human amniotic fluid cells (hAFSCs) are a fascinating foetal cell-type that have important stem cell characteristics; however, they are a heterogeneous population that ranges from totally differentiated or progenitor cells to highly multipotent stem cells. There is no single approach to isolating the stem cell component, but the selection of a subpopulation of hAFSCs expressing c-Kit is widely employed, while a deep characterization of the two populations is still lacking. Here we performed single-cell and bulk RNAseq analysis to compare the gene expression profiles of adherent amniotic fluid cells and their subpopulation c-Kit+. Information deriving from this high throughput technology on the transcriptome was then confirmed for specific targets with protein expression experiments and functional analysis. In particular, transcriptome profiling identified changes in cellular distribution among the different clusters that correlated with significant differential expression in pathways related to stemness, proliferation, and cell cycle checkpoints. These differences were validated by RT-PCR, immunofluorescence, WB, and cell cycle assays. Interestingly, the two populations produced secretomes with different immune-modulating and pro-regenerative potentials. Indeed, the presence of TGFβ, HGF, IDO was higher in EVs deriving from c-Kit+ cells, unlike IL-6. These results suggest the existence of deep intra-population differences that can influence the stemness profile of hAFSCs. This study represents a proof-of-concept of the importance of selecting c-Kit positive fractions with higher potential in regenerative medicine applications.
Altered MANF Expression in Pancreatic Acinar and Ductal Cells in Chronic Alcoholic Pancreatitis: A Cross-Sectional Study
Authors: Caldwell Nicholas J. , Li Hui , Bellizzi Andrew M. , Luo Jia
Published: February 2023

Background: Mesencephalic astrocyte-derived neurotrophic factor (MANF) is an endoplasmic reticulum (ER) stress response protein that plays an important role in pancreatic functions. As both alcohol and ER stress response proteins are involved in the pathogenesis of pancreatitis, we sought to investigate the expression of MANF in chronic alcoholic pancreatitis (CAP) and chronic non-alcoholic pancreatitis (CNP). Methods: A cohort of chronic pancreatitis tissues was gathered from routine surgical pathology (n = 77) and autopsy (n = 10) cases and tissue microarrays were created. Sampled tissues were reviewed and designated as representing CAP (n = 15), CNP (n = 58), or normal pancreatic tissue (NPT) (n = 27). MANF immunohistochemistry (IHC) and digital image analysis were performed to obtain an estimation of tissue fibrosis and an optical density (OD) of MANF IHC in ducts and acini for each case. The averaged values for these variables among histologic designations were compared. Results: The amount of fibrous tissue of the combined CAP and CNP group (chronic alcoholic and non-alcoholic pancreatitis, CANP) exceeded that of the NPT group (70% vs. 34%, p < 0.0001). The MANF OD in ducts of CANP was significantly higher than that of NPT (0.19 vs. 0.10, p < 0.05). The MANF OD in ducts of CAP was significantly higher than that of CNP (0.27 vs. 0.17, p < 0.05). The MANF OD in acini of CAP was significantly lower than that in CNP (0.81 vs. 1.05, p < 0.05). Finally, there was a statistically significant positive relationship between the amount of fibrosis and MANF OD in ducts (p < 0.001). Conclusions: MANF expression was higher in ducts of CAP than CNP. In contrast, MANF expression in acini was lower in CAP than CNP and NPT. There was a positive correlation between fibrosis and MANF levels in the ducts.
Magnetic SERS Strip Based on 4-mercaptophenylboronic Acid-Modified Fe3O4@Au for Active Capture and Simultaneous Detection of Respiratory Bacteria
Authors: Li Jingfei , Chen Jin , Dai Yuwei , Liu Zhenzhen , Zhao Junnan , Liu Shuchen , Xiao Rui
Published: January 2023

The rapid diagnosis and detection of respiratory bacteria at the early stage can effectively control the epidemic spread and bacterial infection. Here, we designed a rapid, ultrasensitive, and quantitative lateral flow immunoassay (LFA) strip for simultaneous detection of respiratory bacteria S. aureus and S. pneumoniae. In this assay, the surface enhanced Raman scattering (SERS) tags were designed through combining magnetite Raman enhancement nanoparticle Fe3O4@Au/DTNB and recognition element 4-mercaptophenylboronic acid (4-MPBA). Further, 4-MPBA could capture multiple bacteria in a complex environmental solution. Based on the strategies, Fe3O4@Au/DTNB-mediated magnetic enrichment and 4-MPBA-mediated universal capture capabilities improved the detection sensitivity, the limits of detection for S. aureus and S. pneumoniae were as low as 8 and 13 CFU mL−1, respectively, which were more sensitive than those of colloidal gold method. The Fe3O4@Au/DTNB/Au/4-MPBA-LFA also exhibited good reproducibility, excellent specificity, and high recovery rates in sputum samples, indicating its potential application in the detection of respiratory bacteria samples.
Experimental and Analytical Study on Performance of Fiber-Reinforced Self-Stressed Concrete
Authors: Krithika P. , Gajalakshmi P. , Revathy J.
Published: January 2023

Expansive cement is a unique type of cement that, when mixed with water, produces a paste which expands in volume significantly more than standard Portland cement. This expansion nullifies the deficit of shrinkage that arises during concrete hardening. This paper presents the outcomes of two phases of experimentation. Phase I of the paper provides a summary of the performance of expansive cement concrete mixes prepared with various proportions of expansive cement, which partially supplements the ordinary Portland cement, and which is infused with varying amounts of PVA fibers of 0.5, 1, 1.5, 2, and 2.5%, as a form of reinforcement under compression, tension, and flexure. Concrete strength, curing effect, and PVA fibers are the variables in Phase I of the study. The Phase II findings provide the buckling behavior of the self-stressed concrete columns reinforced with PVA fibers when the optimum concrete mix obtained from the Phase I investigation was poured inside steel tubes of varying thicknesses of 2 mm and 2.5 mm to restrict the expansion of the concrete, thereby making it self-stressed concrete. The D/t ratio, inclusion of expansive cement, and PVA fibers are the variables for Phase II of the study. The self-stressed columns with 2% PVA fibers showed better performance than the other columns.
Computationally Efficient Method for Steel Column Buckling in Fire
Authors: Kervalishvili Andrei , Talvik Ivar
Published: February 2023

The stability of axially loaded steel columns with compact rectangular hollow sections at elevated temperatures is studied in this paper. The current Eurocode model for checking the buckling resistance of columns in fire was developed on a similar basis to that for ambient conditions. Due to the effect of the complex non-linear behaviour of steel in fire, the standard design model is not always fully appropriate, and certain parameter ranges may give unsafe results. In this work, an analytical method to determine the buckling resistance of steel columns at elevated temperatures is proposed, accounting for variable non-linear stiffness properties which have significant effects on the flexural buckling resistance of steel columns in fire. A finite element model was developed, and an extensive numerical study was performed to explore the effects of different parameters on the behaviours of steel columns at elevated temperatures. The proposed method is validated by comparing the performance with the results of the numerical model. Its improved accuracy with respect to the current Eurocode method is verified. The advantage of the new technique is its computational efficiency, which is valuable in reliability evaluations or data-based design procedures demanding numerous calculation cycles. The potential of the method for probability-based analysis is supported by the format, which enables us to explicitly handle the uncertainties of essential parameters. The proposed framework is suitable for extension to incorporate different material models and section types.
Niclosamide Revitalizes Sorafenib through Insulin-like Growth Factor 1 Receptor (IGF-1R)/Stemness and Metabolic Changes in Hepatocellular Carcinoma
Authors: Peng Syue-Wei , Ngo Mai-Huong T. , Kuo Yung-Che , Teng Ming-Hao , Guo Chin-Lin , Lai Hung-Cheng , Chang Te-Sheng , Huang Yen-Hua
Published: February 2023

Sorafenib is the first approved systemic targeting agent for advanced HCC; however, when used alone, drug resistance can result in considerably reduced efficacy. Here, we demonstrate that niclosamide, an antihelminthic agent approved by the US Food and Drug Administration, can be repurposed to increase sorafenib sensitivity in sorafenib-resistant HCC cells. We generated sorafenib-resistant HCC cell lines (HepG2215_R and Hep3B_R) with elevated IGF-1R levels and strong properties in terms of stemness and epithelial–mesenchymal transition. Niclosamide was found to increase sorafenib sensitivity effectively in both cell lines and their organoids. The underlying mechanism involves the modulation of cancer stemness, IGF-1R/p-IGF1R/OCT4, and metabolic changes. The combination of sorafenib and niclosamide, but not linsitinib, effectively suppressed the IGF-1R/OCT4 expressions, yielded a synergistic combination index (CI), and attenuated stemness-related properties such as secondary tumor sphere formation and cell migration in sorafenib-resistant HCC cells. Notably, niclosamide significantly suppressed the sorafenib-induced IGF-1R phosphorylation prompted by IGF-1 treatment. Niclosamide effectively downregulated the sorafenib-induced gene expression associated with glycolysis (GLUT1, HK2, LDHA, and PEPCK), stemness (OCT4), and drug resistance (ABCG2) and enhanced the ability of sorafenib to reduce the mitochondrial membrane potential in vitro. The synergistic effect of a combination of niclosamide and sorafenib in vivo was further demonstrated by the decreased tumor size and tumor volume resulting from apoptosis regulation. Our results suggest that niclosamide can enhance sorafenib sensitivity in sorafenib-resistant HCC cells through IGF-1R/stemness regulation and metabolic changes. Our findings highlight a practical clinical strategy for enhancing sorafenib sensitivity in HCC.
Evaluation of a Developed MRI-Guided Focused Ultrasound System in 7 T Small Animal MRI and Proof-of-Concept in a Prostate Cancer Xenograft Model to Improve Radiation Therapy
Authors: Zhang Xinrui , Greiser Sebastian , Roy Upasana , Lange Franziska , van Gorkum Robbert , Fournelle Marc , Speicher Daniel , Tretbar Steffen , Melzer Andreas , Landgraf Lisa
Published: February 2023

Focused ultrasound (FUS) can be used to physiologically change or destroy tissue in a non-invasive way. A few commercial systems have clinical approval for the thermal ablation of solid tumors for the treatment of neurological diseases and palliative pain management of bone metastases. However, the thermal effects of FUS are known to lead to various biological effects, such as inhibition of repair of DNA damage, reduction in tumor hypoxia, and induction of apoptosis. Here, we studied radiosensitization as a combination therapy of FUS and RT in a xenograft mouse model using newly developed MRI-compatible FUS equipment. Xenograft tumor-bearing mice were produced by subcutaneous injection of the human prostate cancer cell line PC-3. Animals were treated with FUS in 7 T MRI at 4.8 W/cm2 to reach ~45 °C and held for 30 min. The temperature was controlled via fiber optics and proton resonance frequency shift (PRF) MR thermometry in parallel. In the combination group, animals were treated with FUS followed by X-ray at a single dose of 10 Gy. The effects of FUS and RT were assessed via hematoxylin-eosin (H&E) staining. Tumor proliferation was detected by the immunohistochemistry of Ki67 and apoptosis was measured by a TUNEL assay. At 40 days follow-up, the impact of RT on cancer cells was significantly improved by FUS as demonstrated by a reduction in cell nucleoli from 189 to 237 compared to RT alone. Inhibition of tumor growth by 4.6 times was observed in vivo in the FUS + RT group (85.3%) in contrast to the tumor volume of 393% in the untreated control. Our results demonstrated the feasibility of combined MRI-guided FUS and RT for the treatment of prostate cancer in a xenograft mouse model and may provide a chance for less invasive cancer therapy through radiosensitization.
Electrochemical and Optical Properties of Fluorine Doped Tin Oxide Modified by ZnO Nanorods and Polydopamine
Authors: Viter Roman , Fedorenko Viktoriia , Gabriunaite Inga , Tepliakova Irina , Ramanavicius Simonas , Holubnycha Viktoriia , Ramanavicius Arunas , Valiūnienė Aušra
Published: February 2023

Various forms of zinc oxide (ZnO) are frequently used in the design of optical and electrochemical sensors. However, the optical and electrochemical properties of ZnO should be properly adjusted depending on the application area. Therefore, in this work, we have investigated changing/tuning the properties of ZnO by depositing a layer of polydopamine (PDA) on its surface. In order to perform this investigation, the surface of fluorine-doped tin oxide (FTO) was modified with the layer of ZnO nanorods and PDA. ZnO nanorods were synthesized by hydrothermal synthesis technique, and after the synthesis, they were coated with polydopamine exploiting the self-polymerization of dopamine. The nanostructures were investigated by using electrochemical and optical methods. Electrochemical impedance spectroscopy measurements showed that electrochemical properties of FTO-ZnO and FTO-ZnO-PDA nanostructures could be changed by the variation of both—applied electrical potential and/or exposition towards lighting. Interaction between ZnO-PDA and bovine serum albumin (BSA) molecules has been investigated by (photo)electrochemical and photoluminescence methods. A mechanism of possible interaction between BSA and the ZnO-PDA surface has been proposed.
Placing Ureaplasma within the Context of Bronchopulmonary Dysplasia Endotypes and Phenotypes
Authors: Van Mechelen Karen , van Westering-Kroon Elke , Hütten Matthias , Mahieu Ludo , Villamor Eduardo
Published: January 2023

Different pathophysiological pathways (endotypes), leading to very preterm birth may result in distinct clinical phenotypes of bronchopulmonary dysplasia (BPD). Ureaplasma is a unique player in the pathogenesis of BPD. The interaction between factors inherent to Ureaplasma (virulence, bacterial load, duration of exposure), and to the host (immune response, infection clearance, degree of prematurity, respiratory support, concomitant infections) may contribute to BPD development in a variable manner. The data reviewed herein support the hypothesis that Ureaplasma, as a representative of the infectious/inflammatory endotype, may produce pulmonary damage predominantly in parenchyma, interstitium, and small airways. In contrast, Ureaplasma may have a very limited role in the pathogenesis of the vascular phenotype of BPD. In addition, if Ureaplasma is a key factor in BPD pathogenesis, its eradication by macrolides should prevent BPD. However, various meta-analyses do not show consistent evidence that this is the case. The limitations of current definitions and classifications of BPD, based on respiratory support needs instead of pathophysiology and phenotypes, may explain this and other failures in strategies aimed to prevent BPD. The precise mechanisms through which Ureaplasma infection leads to altered lung development and how these pathways can result in different BPD phenotypes warrant further investigation.
Diet Quality and Exhaled Breath Condensate Markers in a Sample of School-Aged Children
Authors: Rodrigues Mónica , de Castro Mendes Francisca , Paciência Inês , Cavaleiro Rufo João , Silva Diana , Delgado Luís , Moreira André , Moreira Pedro
Published: January 2023

Exhaled breath condensate (EBC) analysis is a recently developed, non-invasive method used to identify and quantify biomarkers, mainly those coming from the lower respiratory tract. It seems that diet can influence the airway’s inflammation and change the exhaled breath composition. This study aimed to assess the association between diet quality intake and markers in EBC among school-aged children. A cross-sectional analysis included 150 children (48.3% females, aged 7–12 years, mean age: 8.7 ± 0.8 years) from 20 schools across Porto, Portugal. We assessed diet quality through the Healthy Eating Index (HEI)-2015, which was estimated based on a single 24 h food recall questionnaire. EBC samples were collected, and we assessed their ionic content (Na+, K+) and conductivity. The association between diet quality and Na+, K+, Na+/K+ ratio and conductivity was estimated using logistic regression models adjusted for potential confounders. After adjustment, a higher quality diet score increases the odds of higher conductivity values of the EBC (aOR = 1.04, 95%CI 1.00; 1.08). Our findings suggest that a higher diet quality in school-aged children is associated with higher conductivity levels of the EBC.
Knowledge and Protective Behaviors of Teachers on Skin Cancer: A Cross-Sectional Survey Study from Turkey
Authors: Kus Celal , Kus Mine Mujde , Keten Hamit Sirri , Ucer Huseyin , Guvenc Numan , Kus Fatih , Yildirim Hasan Cagri , Akbayram Hatice Tuba
Published: February 2023

Background: People socialize and receive education and training for the first time outside the home at school, where their teachers act as role models. Teachers play a crucial role in instilling sun-protection habits in children. Avoiding the sun between 10 a.m. and 4 p.m., staying in the shade, wearing sun-protective clothing, wearing sunglasses, wearing hats, using sunscreen products, and using an umbrella are some of the sun-protection methods described in the literature. This study sought to assess teachers’ skin cancer knowledge and attitudes (SC). Methods: In this cross-sectional study conducted between 21 September 2020, and 21 October 2020, 647 teachers from 30 schools in Kahramanmaras were included with their consent. The number of teachers employed at Kahramanmaras was 1863. Accordingly, the sample was found to be 641 with a 5% margin of error and a 99% confidence interval. Schools were selected by a simple random method. Teachers’ knowledge and behaviors were assessed using a 25-point questionnaire designed by the literature data to gauge the level of SC knowledge. Results: Of the 647 teachers included in this study, 230 (35.5%) were male, and 417 (64.5%) were female. The mean age of the participants was 38.44 ± 8.79 (min = 22, max = 65) years. The knowledge level of the teachers about SC was 13.54 ± 4.48 (min = 0, max = 23). The internet (75.9%) was the most preferred source of information. SC knowledge level was significantly higher in those with SC in their families and birthmarks on their bodies. (p < 0.001, p = 0.042, respectively). The rate of precaution taken to protect from the sun was higher in those with higher knowledge (p = 0.032). Women, primary school teachers, those with skin type 1, those with multiple nevi, and those with a high level of SC knowledge used statistically significantly more sunscreen (p = 0.001, p = 0.003, p < 0.001, p = 0.037, p = 0.002, respectively). Conclusions: It was found that the knowledge level of teachers about skin cancer and sun-protective behaviors was moderate. Correct behaviors increased as knowledge about SC grew. Information and recommendations made on the Internet should be made by experts. Additionally, health policymakers should implement projects aimed at improving teachers’ knowledge and behaviors and, through them, teaching students about SC; as such projects would significantly contribute to both public health and health economics.
Innovations in the Packaging of Meat and Meat Products—A Review
Authors: Gil Marian , Rudy Mariusz
Published: February 2023

This study aims to systematize the knowledge about innovative solutions to understand the composition of packaging materials and bioactive substances used in the packaging processes of meat and meat products, given the contemporary trends and consumer expectations. In edible packaging, the application of natural and renewable biopolymers is gaining popularity as, unlike petroleum-based plastic packaging materials, they do not cause environmental problems. Packaging using active compounds further extends the shelf life of food products compared with traditional packaging by reducing the adverse effects during storage, such as oxidation, microbial growth, and moisture loss. On the other hand, the inclusion of natural bioactive substances in packaging provides an opportunity to increase the shelf life of food products and/or decrease the use of preservatives. This direction offers a wide field for research due to the multitude of substances, their impact, and the properties of the packaged product.
The Crane Flies of Martinique, with the Description of Four New Species (Diptera, Tipuloidea)
Authors: Mederos Jorge , Pollet Marc , Oosterbroek Pjotr
Published: February 2023

The results of a first major inventory of the crane flies (Diptera: Tipuloidea) of the Lesser Antillean island of Martinique are presented here, based almost exclusively on pan trap sampling during 2018. A total of 27 species of crane flies were discovered, including four new species, Teucholabis (Teucholabis) carbetensis sp. nov., Atypophthalmus (Atypophthalmus) vanewrighti sp. nov., Rhipidia (Rhipidia) martiniquensis sp. nov. (all three Limoniidae), and Zelandotipula gelhausi sp. nov. (Tipulidae). Seven species have been identified till the morphospecies level, due to the exclusive capture of female specimens. In addition, the species Zelandotipula parviceps (Speiser, 1909) from Guadeloupe is redescribed, based on the study of the female holotype. A checklist of Limoniidae and Tipulidae of Martinique is provided.
Lizard Dewlap Color and Malaria Infection: Testing the Hamilton-Zuk Hypothesis
Authors: Doan Tiffany M. , Mingos Alexis D. , Juge Aiden E. , Simmons Melissa A.
Published: February 2023

The Hamilton-Zuk hypothesis indicates that parasites may have a negative effect on the appearance of sexual traits within an infected individual. Anolis sagrei, or brown anoles, are small invasive lizards common throughout Florida and many other areas where they have been introduced. The colorful dewlaps under the chins of males are used as a signal during territorial contests with other males and as an ornament to attract females. Anoles may be infected by the malaria parasite Plasmodium floridense. In this study, we investigated the relationship of malarial infection with dewlap color and spectral brightness of A. sagrei. We achieved this by capturing male brown anoles, taking blood samples to examine for malarial infection, and recording the color of their dewlaps. We found that the dewlaps of infected Anolis sagrei had duller coloration than the dewlaps of uninfected lizards. These results provide support for the Hamilton-Zuk hypothesis. Our results suggest that infection by P. floridense could potentially influence fitness of the lizards by reducing the expression of their color signals. Additional research on fitness effects of the malaria parasite on anoles is important for fully understanding this phenomenon.
An Underrecognized Histologic Clue to the Diagnosis of Mucous Membrane Pemphigoid: A Case Report and Review of Diagnostic Guidelines
Authors: McFadden Jason R. , Chaudhari Advaita S. , Birenbaum Debra , Margesson Lynette , Gonzalez Jorge , Sriharan Aravindhan
Published: February 2023

Mucous membrane pemphigoid (MMP), also known as cicatricial pemphigoid (CP), is a heterogeneous group of subepidermal blistering diseases that affect the mucous membranes, most frequently in the eye and oral cavity. MMP is often unrecognized or misdiagnosed in its early stages due to its rarity and nonspecific presentation. We present the case of a 69-year-old female in which MMP of the vulva was not initially suspected. The first biopsy, from lesional tissue for routine histology, revealed fibrosis, late-stage granulation tissue, and nonspecific findings. A second biopsy, from perilesional tissue for direct immunofluorescence (DIF), revealed DIF findings typical of MMP. Scrutiny of both the first and second biopsies revealed a subtle but telling histologic feature: subepithelial clefts along adnexae in the context of a scarring process with neutrophils and eosinophils, which can be an important clue to MMP. This histologic clue has been previously described; reinforcing its importance may prove useful for future cases, especially those for which DIF is not feasible. Our case demonstrates the protean presentations of MMP, the need for persistence in sampling unusual cases, and the relevance of inconspicuous histologic features. The report highlights this underrecognized yet potentially decisive histologic clue to MMP, reviews current biopsy guidelines when MMP is suspected, and delineates the clinical and morphological features of vulvar MMP.
Airfoil Shape Morphing through a Novel Parameterization and Fitting Optimization Method Based on Uniform Non-Rational B-Spline Functions
Authors: Tortora Giancarlo , Concilio Antonio , Pecora Rosario
Published: February 2023

The aim of this work is to implement an innovative parameterization and fitting procedure for the definition of a mathematical model useful to describe a wide range of airfoils. They are partitioned into three sections: central box, leading edge, and trailing edge. Each section is mathematically represented by two opposed, uniform, non-rational B-spline curves, describing the upper and lower airfoil segments’ perimeter. A novel approach is used to ensure both the desired continuity between two adjacent segments (up to 2nd derivatives) and sufficient model versatility and flexibility while managing a limited number of parameters, defining tangent and curvature vectors as scale factor variables. These parameters allow for a variable separation approach during the geometric fitting procedure that can be carried out considering two nested optimization processes, one based on a genetic algorithm and the other on a numerical gradient evaluation of the objective function. The representation method has been verified against different airfoils, comparing the geometric and aerodynamic properties of the input and model-based generated profile. To show the mathematical model’s capabilities and possible applications, a comparison between existing and proposed airfoil approximation methods has been provided together with examples of “global” and “local” morphing and CFD analyses of the resulting airfoils.
A Preliminary Analysis of In-Situ Stress at Mount Meager by Displacement Discontinuity Method with Topography and Tectonics Considered
Authors: Chai Yutong , Chen Zhuoheng , Yin Shunde
Published: January 2023

Geothermal energy is one of the most stable and clean solutions to replace the traditional fossil fuel energy resource. The South Meager geothermal prospect, located in southwestern British Columbia, contains large geothermal energy resources due to recent volcanic activities. The in-situ stress state in the prospective area that influences the characteristics of fractures, thus affecting productivity, remains unknown. In this paper, we present a preliminary analysis of in-situ stress induced by gravitational load at Mount Meager, with tectonics considered. The in-situ stress model was constructed with 3D displacement discontinuity method based on the site-specific topography. The 3D model reveals that the impact of topography is more prominent in shallow and deep regions, while the impact of tectonics is prominent in an intermediate depth range. With the implementation of inferred tectonic stress state, normal faulting regime takes place at shallow depth (<800 m); at the intermediate depth (800–1600 m), the transition of faulting regime from normal to strike-slip and then to thrust occurs; at deeper depth (>1800 m), the fault type becomes normal again. The orientations of major and minor induced horizontal stresses transition from varying with local topography to perfectly aligned with the orientations of major and minor horizontal tectonic stresses at NWN-SES 330° and NEE-SWW 60°, respectively, as depth increases.
Examining the Causality between Integrated Reporting and Stock Market Capitalization. The Case of the European Renewable Energy Equipment and Services Industry
Authors: Sahlian Daniela Nicoleta , Popa Adriana Florina , Nicoară Ștefania Amalia , Bâtcă-Dumitru Corina Graziella
Published: January 2023

The International Accounting Standards Board (IASB) and the International Sustainability Standards Board (ISSB) of the IFRS Foundation support the integrated reporting of companies’ financial and sustainability performance to stakeholders. This paper aims to investigate whether financial and environmental, social, and corporate governance (ESG) practices have a real impact on the success of the companies in the European renewable energy equipment and services industry. Using the Granger test, the causality between the market capitalization and financial indicators was established, whereas no causality was identified between the market capitalization and ESG performance. The research led to the conclusion that the investment decision is mainly based on the information provided by the financial statements of the companies, the early stages of sustainability reporting regulation, and the need for increasing the quality and availability of corporate social responsibility information for investors.
Understanding Atmospheric Convection Using Large Eddy Simulation
Authors: Dogra Gaurav , Dewan Anupam , Sahany Sandeep
Published: February 2023

Cloud formation is based on the fundamental principle of atmospheric convection, which involves the vertical transport of heat and moisture into an unstable environment. Convective transfer of moisture and heat in the form of turbulent fluxes over the Bay of Bengal (BoB) has not been explored much and is not resolved in global and regional climate models (GCMs and RCMs) due to the coarser grid resolutions used. Therefore, the present study is an attempt to understand the convection phenomenon over the BoB using a high-resolution cloud-resolving large eddy simulation. Due to the lack of observational data over the BoB, initial and boundary conditions were generated using reanalysis data. We found that the LES successfully captured the cloud formation and convection phenomenon. The turbulence in the convection was analyzed by using Reynolds averaging to obtain variances and covariances. The presence of turbulence over the region was observed. The cloud characteristics were verified by conditionally averaging the output fields. The present study paves a pathway to perform various simulations at different atmospheric conditions over the region in order to create a library of high-resolution simulations.
Approximate Analytical Methods for a Fractional-Order Nonlinear System of Jaulent–Miodek Equation with Energy-Dependent Schrödinger Potential
Authors: Alshammari Saleh , Al-Sawalha M. Mossa , Shah Rasool
Published: February 2023

In this paper, we study the numerical solution of fractional Jaulent–Miodek equations with the help of two modified methods: coupled fractional variational iteration transformation technique and the Adomian decomposition transformation technique. The Jaulent–Miodek equation has applications in several related fields of physics, including control theory of dynamical systems, anomalous transport, image and signal processing, financial modelings, nanotechnology, viscoelasticity, nanoprecipitate growth in solid solutions, random walk, modeling for shape memory polymers, condensed matter physics, fluid mechanics, optics and plasma physics. The results are presented as a series of quickly converging solutions. Analytical solutions have been performed in absolute error to confirm the proposed methodologies are trustworthy and accurate. The generated solutions are visually illustrated to guarantee the validity and applicability of the taken into consideration algorithm. The study’s findings show that, compared to alternative analytical approaches for analyzing fractional non-linear coupled Jaulent–Miodek equations, the Adomian decomposition transform method and the variational iteration transform method are computationally very efficient and accurate.
The Role of Flaxseed in Improving Human Health
Authors: Nowak Wioletta , Jeziorek Małgorzata
Published: January 2023

Flaxseed contains high amounts of biologically active components such as α-linolenic acid, lignans, and dietary fiber. Due to its numerous nutritional properties, flaxseed has been classified as a “superfood”, that is, a food of natural origin with various bioactive components and many health-promoting benefits. Flaxseed consumption can be an important factor in the prevention of diseases, particularly related to nutrition. The regular consumption of flaxseed may help to improve lipid profile and lower blood pressure, fasting glucose, and insulin resistance index (HOMA-IR). Moreover, flaxseed is characterized by anticancer and antioxidant properties and can significantly reduce the intensity of symptoms associated with menopause, constipation, and mental fatigue, improve skin condition, and accelerate wound healing. In addition to its bioactive compounds, flaxseed also contains antinutrients such as cyanogenic glycosides (CGs), cadmium, trypsin inhibitors, and phytic acid that can reduce the bioavailability of essential nutrients and/or limit its health-promoting effects. Three common forms of flaxseed available for human consumption include whole flaxseed, ground flaxseed, and flaxseed oil. The bioavailability of ALA and lignans is also dependent on the form of flaxseed consumed. To ensure high bioavailability of its bioactive components, flaxseed should be consumed in the ground form.
Identifying the Group Vulnerable to Unmet Medical Needs Due to Food Security: According to Children in the Household
Authors: Kim Min-Soo , Lee Hyeon-Ji , Kim Jae-Hyun
Published: February 2023

Objective: Adults may experience unmet medical needs for various reasons. The purpose of this study was to examine the effect of food security on unmet medical needs according to the presence of children in the household of adults, as well as to identify the medically vulnerable group considering individual and household characteristics. Methods: This study was conducted using data from the National Health and Nutrition Examination Survey for 2013–2015 and 2019–2020. The subjects of the study were 23,069 adults 19 years of age or older, and were divided into two groups according to whether or not children were included in the household. In order to observe the association between food security and unmet medical needs, multiple logistic regression analysis was performed. In addition, a subgroup analysis was performed in consideration of individual and household characteristics. Results: When food security was unstable for households with children, or without children, there was a high correlation with unmet medical needs. Considering individual and household characteristics, in groups with lower age and household income level, or higher number of members in household and subjective health status, food security was strongly correlated with unmet medical needs in households with children. Contrarily, households without children showed a high correlation in the opposite characteristics of households with children, excluding household income level. Conclusion: Food security was highly correlation with unmet medical needs regardless of whether or not children were included in the household. However, according to the individual and household characteristics of households with and without children, the relationship between food security and unmet medical needs was found to be different. Therefore, it is necessary to prepare a health policy that can increase access to medical services in consideration of food security and individual and household characteristics depending on whether or not children are included in the household.
Factors Affecting Preventive Behaviors for Safety and Health at Work during the COVID-19 Pandemic among Thai Construction Workers
Authors: Khaday Samsiya , Li Kai-Way , Dorloh Halimoh
Published: February 2023

Occupational health and safety risks are of major concerns in construction industry. The COVID-19 outbreak provides an additional risk that could drastically affect the safety risks and health of construction workers. Understanding the factors that affect the health and safety of construction workers is significant in reducing risky behaviors and enhancing worker preventive behaviors. Via integrating the Protection Motivation Theory (PMT) and the extended Theory of Planned Behavior (TPB), this study investigates the factors that affect preventive behaviors among construction workers during the COVID-19 pandemic in Bangkok, Thailand. A total of 610 Thai construction workers participated in an online questionnaire survey, which consisted of nine factors with 43 questions. Structure equation modeling (SEM) was adopted to analyze the causal relationships among the latent variable. The SEM results indicated that organizational support and knowledge about COVID-19 had significant (p < 0.0001) direct influences on perceived vulnerability and perceived severity. In addition, perceived vulnerability and perceived severity had significant direct influences on perceived behavioral control. Perceived severity had significant (p < 0.0001) direct influence on attitude towards behavior. Moreover, perceived behavioral control and attitude towards behavior had significant (p < 0.0001) direct influence on intention to follow the preventive measure. Furthermore, the intention to follow the preventive measure had significant (p < 0.0001) direct influences on the COVID-19 preventive behavior. Of note, organizational support and knowledge about COVID-19 had significant (p < 0.0001) indirect influence on COVID-19 preventive behavior. The findings of this study may assist project managers/supervisors and authorities in the construction industry in understanding the challenge during COVID-19 and possible similar epidemics in the future. In addition, conducting effective strategies would improve construction industry safety and promote preventive behaviors among construction workers.
Impact of Irrigation with Contaminated Water on Heavy Metal Bioaccumulation in Water Chestnut (Trapa natans L.)
Authors: Taher Mostafa A. , Zouidi Ferjeni , Kumar Pankaj , Abou Fayssal Sami , Adelodun Bashir , Goala Madhumita , Kumar Vinod , Andabaka Željko , Širić Ivan , Eid Ebrahem M.
Published: February 2023

This study investigated the monitoring of six heavy metals (Cd, Cr, Cu, Fe, Mn, and Zn) in pond water, sludge, and cultivated water chestnut (Trapa natans L.) crops in Saharanpur district of Uttar Pradesh, India. For this purpose, samples of pond water, sludge, and T. natans plant (nut, shoot, and root) were collected in November of 2021 and 2022 from three zones (Zone 1: agricultural area irrigated with borewell water, Zone 2: urban area irrigated with municipal wastewater, and Zone 3: rural area irrigated with mixed type of wastewater) and subsequently analyzed for heavy metal concentration using atomic absorption spectroscopy. The results showed that the physicochemical and heavy metal characteristics of pond water and sludge were significantly (p < 0.05) higher in Zone 2 and 3 than to those in Zone 1. The concentration of heavy metals in T. natans root was comparatively high followed by shoot and nut parts. The bioaccumulation factor (BAF) was maximum while using pond water as a reference medium compared to pond sludge. Overall, the increasing order of heavy metals in T. natans was observed as Cd < Cr < Cu < Zn < Mn < Fe. In the edible part (nut) of T. natans, the average contents of Cd (0.005 ± 0.002 mg/kg), Cr (0.134 ± 0.009 mg/kg), Cu (1.043 ± 0.104 mg/kg), Fe (9.589 ± 0.957 mg/kg), Mn (4.326 ± 0.753 mg/kg), and Zn (1.540 ± 0.537 mg/kg) were comparatively less than shoot and root parts. Overall, the results revealed that T. natans irrigated with contaminated irrigation supplies at Zone 2 and 3 showed the highest BAF of heavy metals than Zone 1. Because the heavy metal concentrations in edible parts of T. natans did not exceed the threshold limits, the contaminated water sources in the Saharanpur region of India should be used in a safe and controlled manner.