bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamics of Coffee Certifications in Producer Countries: Re-Examining the Tanzanian Status, Challenges and Impacts on Livelihoods and Environmental Conservation
Authors: Kangile Joseph Rajabu , Kadigi Reuben M. J. , Mgeni Charles Peter , Munishi Bernadetha Pantaleo , Kashaigili Japhet , Munishi Pantaleo K. T.
Published: September 2021

Certification is increasingly becoming necessary for accessing coffee export markets and practicing environmental conservation, especially at this time when many of the farmers in developing countries strive to achieve agricultural transformation. Using data from 400 randomly selected coffee farmers in Tanzania, the study determined the status, constraints, key drivers, and impact of coffee certifications. Descriptive statistics and the endogenous switching regression (ESR) model were used for data analysis. Results indicated that the level of coffee certification is low, being constrained by unawareness and inaccessibility, the prevalence of coffee diseases, failure in realizing price advantages, and certification not being cost effective. Economies of scale, experience, and participation in collective actions are significant factors affecting coffee farmers’ decision to join certification schemes. Additionally, the study rejects the hypothesis of certification to improve household income. However, certification improved awareness and practices of environmental conservation among coffee farmers. It is thus important to embark on awareness creation and make certification services accessible and cost effective to coffee farmers for increased access to niche export markets. Easing transmission of price premiums to coffee farmers will also increase the supply of sustainably grown coffee, improve coffee farmers’ livelihood, and help in the attainment of environmental sustainability goals within the coffee supply chain.
Silicon Application Induced Alleviation of Aluminum Toxicity in Xaraés Palisadegrass
Authors: Baggio Guilherme , Dupas Elisângela , Galindo Fernando Shintate , Megda Marcio Mahmoud , Pereira Nathália Cristina Marchiori , Luchetta Monique Oliveira , Tritapepe Caio Augusto , da Silva Marcelo Rinaldi , Jalal Arshad , Teixeira Filho Marcelo Carvalho Minhoto
Published: September 2021

Aluminum (Al) toxicity is a major abiotic constraint for agricultural production in acidic soils that needs a sustainable solution to deal with plant tolerance. Silicon (Si) plays important roles in alleviating the harmful effects of Al in plants. The genus Urochloa includes most important grasses and hybrids, and it is currently used as pastures in the tropical regions. Xaraés palisadegrass (Urochloa brizantha cv. Xaraés) is a forage that is relatively tolerant to Al toxicity under field-grown conditions, which might be explained by the great uptake and accumulation of Si. However, studies are needed to access the benefits of Si application to alleviate Al toxicity on Xaraés palisadegrass nutritional status, production, and chemical–bromatological composition. The study was conducted under greenhouse conditions with the effect of five Si concentrations evaluated (0, 0.3, 0.6, 1.2, and 2.4 mM) as well as with nutrient solutions containing 1 mM Al in two sampling dates (two forage cuts). The following evaluations were performed: number of tillers and leaves, shoot biomass, N, P, K, Ca, Mg, S, B, Cu, Fe, Mn, Zn, Al, and Si concentration in leaf tissue, Al and Si concentration in root tissue, neutral detergent fiber (NDF), and acid detergent fiber (ADF) content in Xaraés palisadegrass shoot. Silicon supply affected the relation between Si and Al uptake by increasing root Al concentration in detriment to Al transport to the leaves, thereby alleviating Al toxicity in Xaraés palisadegrass. The concentrations between 1.4 and 1.6 mM Si in solution decreased roots to shoots Al translocation by 259% (from 3.26 to 1.26%), which contributed to a higher number of leaves per plot and led to a greater shoot dry mass without affecting tillering. Xaraés palisadegrass could be considered one of the greatest Si accumulator plants with Si content in leaves above 4.7% of dry mass. In addition, Si supply may benefit nutrient-use efficiency with enhanced plant growth and without compromising the chemical–bromatological content of Xaraés palisadegrass.
Distribution of Deer Keds (Diptera: Hippoboscidae) in Free-Living Cervids of the Tuscan-Emilian Apennines, Central Italy, and Establishment of the Allochthonous Ectoparasite Lipoptena fortisetosa
Authors: Andreani Annalisa , Stancampiano Laura , Belcari Antonio , Sacchetti Patrizia , Bozzi Riccardo , Ponzetta Maria Paola
Published: September 2021

Lipoptena fortisetosa and L. cervi are hematophagous ectoparasites belonging to the Hippoboscidae family and preferentially living on cervids. In recent years, they have received specific attention due to the great increase in the abundance of their host species, and to their medical and veterinary importance as possible vectors of pathogens harmful to humans and animals. The aim of this study was to investigate the parasitism level of both of these flies on their main hosts in Italy, which are red deer, fallow deer, and roe deer, and to highlight a possible preference for a species, sex, or age class among the hosts. Deer keds were collected by examining 326 cervids hunted in the Tuscan-Emilian Apennines. Outcomes showed that L. fortisetosa has greatly spread throughout the study area, where it competes with the autochthonous L. cervi. Moreover, red deer was the favored host species of both ectoparasites, while different preferences for host sex and age classes were observed in the two hippoboscids. The regular monitoring of deer ked populations, especially the allochthonous L. fortisetosa, which is continuously spreading in Europe, is recommended to expand the knowledge on these parasitic species that are potentially dangerous to public health.
Preliminary Examination of the Biological and Industry Constraints on the Structure and Pattern of Thoroughbred Racing in New Zealand over Thirteen Seasons: 2005/06–2017/18
Authors: Legg Kylie A. , Gee Erica K. , Cochrane Darryl J. , Rogers Chris W.
Published: September 2021

This study aimed to examine thirteen seasons of flat racing starts (n = 388,964) in the context of an ecological system and identify metrics that describe the inherent characteristics and constraints of the New Zealand Thoroughbred racing industry. During the thirteen years examined, there was a 2–3% per year reduction in the number of races, starts and number of horses. There was a significant shift in the racing population with a greater number of fillies (aged 2–4 years) having a race start, and subsequent longer racing careers due to the inclusion of one more racing preparation post 2008 (p < 0.05). Additionally, there was an increasingly ageing population of racehorses. These changes resulted in more race starts in a career, but possibly because of biological constraints, there was no change in the number of race starts per season, starts per preparation, or days spelling between preparations (p < 0.05). There was no change in the proportion of horses having just one race start (14% of new entrants), indicating that the screening for suitability for a racing career remained consistent. These data identify key industry parameters which provide a basis for future modelling of intervention strategies to improve economic performance and reduce horse injury. Consideration of the racing industry as a bio-economic or ecological model provides framework to test how the industry may respond to intervention strategies and signal where changes in system dynamics may alter existing risk factors for injury.
A Review on Environmental Contaminants-Related Fertility Threat in Male Fishes: Effects and Possible Mechanisms of Action Learned from Wildlife and Laboratory Studies
Authors: Alavi Sayyed Mohammad Hadi , Barzegar-Fallah Sepideh , Rahdar Parastoo , Ahmadi Mohammad Mahdi , Yavari Mina , Hatef Azadeh , Golshan Mahdi , Linhart Otomar
Published: September 2021

Increasing global rates of diminished fertility in males has been suggested to be associated with exposure to environmental contaminants (ECs). The aquatic environments are the final repository of ECs. As the reproductive system is conserved in vertebrates, studies on the effects of ECs on fertility endpoints in fishes provide us with valuable information to establish biomarkers in risk assessment of ECs, and to understand the ECs-related fertility threat. The aim of the present review was to evaluate associations between ECs and fertility determinants to better understand ECs-related male fertility threat in male fishes. Wildlife studies show that the reproductive system has been affected in fishes sampled from the polluted aquatic environment. The laboratory studies show the potency of ECs including natural and synthetic hormones, alkylphenols, bisphenols, plasticizers, pesticides, pharmaceutical, alkylating, and organotin agents to affect fertility determinants, resulting in diminished fertility at environmentally relevant concentrations. Both wildlife and laboratory studies reveal that ECs adverse effects on male fertility are associated with a decrease in sperm production, damage to sperm morphology, alternations in sperm genome, and decrease in sperm motility kinetics. The efficiency of ECs to affect sperm quality and male fertility highly depends on the concentration of the contaminants and the duration of exposure. Our review highlights that the number of contaminants examined over fertility tests are much lower than the number of contaminants detected in our environment. The ECs effects on fertility are largely unknown when fishes are exposed to the contaminants at early developmental stages. The review suggests the urgent need to examine ECs effects on male fertility when a fish is exposed at different developmental stages in a single or combination protocol. The ECs effects on the sperm genome are largely unknown to understand ECs-related inheritance of reproductive disorders transmitted to the progeny. To elucidate modes of action of ECs on sperm motility, it is needed to study functional morphology of the motility apparatus and to investigate ECs-disrupted motility signaling.
Oocyte Morphometric Assessment and Gene Expression Profiling of Oocytes and Cumulus Cells as Biomarkers of Oocyte Competence in Sheep
Authors: Maside Carolina , Sánchez-Ajofrín Irene , Medina-Chávez Daniela , Alves Benner , Garde José Julián , Soler Ana Josefa
Published: September 2021

Oocyte quality is crucial for subsequent embryo development and so it is a major challenge in assisted reproductive technologies. The aim of the present work was to evaluate the morphometric parameters of oocytes (experiment 1) and the relative gene expression of oocytes and cumulus cells (CCs) (experiment 2) as biomarkers of oocyte quality after individually culturing them (one oocyte or embryo/drop). In experiment 1, individually matured oocytes were measured and classified into small, intermediate, and large oocytes after a cluster analysis, based on total diameter (with zona pellucida, ZP), oocyte diameter (without ZP), and ZP thickness. These oocytes were individually fertilized in vitro and cultured. The embryo development was evaluated up to the blastocyst stage. According to the total diameter, oocyte diameter, and ZP thickness, the blastocyst rate decreased in the small oocytes group (3.1 ± 3.1, 14.1 ± 9.4, and 26.7 ± 3.9, respectively) compared to the intermediate (29.4 ± 5.2, 30.5 ± 10.1, and 28.6 ± 9.6, respectively) and large oocytes groups (54.2 ± 13.5, 44.4 ± 3.9, and 67.6 ± 12.4, respectively). In addition, the probability of reaching the blastocyst stage was positively related to the total diameter (p < 0.001), oocyte diameter (p < 0.05), and ZP thickness (p < 0.001). Furthermore, the relative gene expression of BAX, BCL2, GDF9, and GJA1 was lower in oocytes classified as large. In experiment 2, the mRNA transcript relative abundance pattern of genes in CCs was evaluated according to oocyte total diameter and developmental stage reached. CCs from oocytes classified as large and oocytes capable of developing to the blastocyst stage had a lower relative expression of BAX, STAR, and PTGS2, while a higher expression of HAS2 and SDC2 transcript was observed for those oocytes. In conclusion, oocyte morphometric parameters and gene expression analysis in oocytes and CCs provide methods for the identification of the most competent oocytes for assisted reproductive technologies in sheep.
Animal Welfare Assessment in Antibiotic-Free and Conventional Broiler Chicken
Authors: Iannetti Luigi , Romagnoli Sara , Cotturone Giuseppe , Podaliri Vulpiani Michele
Published: September 2021

The poultry sector is moving towards antibiotic-free production, both to challenge the increasing spread of the antibiotic resistance phenomenon and to meet market demands. This could negatively impact the health and welfare of the animals. In this study, the welfare of 14 batches of 41–47-day-old broilers raised by the same integrated company with and without antibiotics was assessed using the Welfare Quality® protocol. The total welfare score did not significantly differ between the two systems: the good-feeding principle was, on average, higher in the conventional batches, with statistical significance (t = −2.45; p = 0.024), while the other welfare principles (good housing, good health and appropriate behaviour) were slightly better in the antibiotic-free batches. Despite stocking densities averagely higher in the antibiotic-free batches, the absence of antibiotics did not seem to impact the good-health principle; in particular, hock burns, foot pad dermatitis and lameness were significantly less severe in the antibiotic-free batches (p < 0.0001, p = 0.018, p < 0.0001, respectively), which showed also a lower death rate (2.34% vs. 2.50%). Better management of antibiotic-free batches was reported, particularly concerning litter conditions. Further studies would be required to identify and standardise a set of managerial methodologies in order to improve the health of broilers raised without antibiotics.
Microbial Species Isolated from Infected Wounds and Antimicrobial Resistance Analysis: Data Emerging from a Three-Years Retrospective Study
Authors: Puca Valentina , Marulli Roberta Zita , Grande Rossella , Vitale Irene , Niro Antonietta , Molinaro Gina , Prezioso Silvia , Muraro Raffaella , Di Giovanni Pamela
Published: September 2021

The antimicrobial resistance is a topic of global interest in the treatment of wound infections. The goal of this retrospective study was both the identification of the microorganisms responsible for wound infections and the determination of their drug susceptibility pattern. The study was performed from 2017 to 2019 and included 239 patients. Thirty-four species were isolated by culture methods and identified and analysed for their susceptibility patterns to antimicrobials through the Walk Away automated system. The presence of one species was the most frequent condition (75.3%), whereas a co-infection was detected in 24.7% of samples. The most common species were Gram-negative (57.9%), amongst which the most prevalent were Pseudomonas aeruginosa (40.2%), Escherichia coli (20.7%), Proteus mirabilis (11.2%), and Acinetobacter baumannii/haemolyticus (9.5%). Gram-positive bacteria were observed in 36.6%, Staphylococcus aureus (79.4%) being the most predominant species. At least one resistance to antibiotics was detected in 88.2% of isolates, while a multi-drug-resistance versus no less than 6 antimicrobials was detected in 29.2% of isolates. Although multi-drug resistant species and co-infections were observed, those were less frequently observed at the wound site. These conditions make the microorganisms eradication more difficult. The detection of a polymicrobial infection and multi-drug resistant microorganisms followed by a proper therapeutic treatment would lead to the resolution of the infection, promoting wound healing and the limitation of the spread of antibiotic resistance.
Carriage of Multidrug-Resistant Bacteria in Healthy People: Recognition of Several Risk Groups
Authors: Neut Christel
Published: September 2021

The increase in multidrug-resistant (MDR) bacteria in hospitalized people and the hospital environment has been thoroughly documented. In contrast, little is known about their presence in the community. However, increasing evidence is showing a high level of carriage in people without infectious signs. Colonized people can later develop infections due to MDR bacteria and may be able to transmit them to susceptible people (the number of which is increasing worldwide), for example, people with comorbidities such as diabetes, cancer, or inflammatory diseases and those in extreme age groups. Risk factors for the acquisition of MDR bacteria are as follows: (1) residence or travel in countries with high levels of MDR bacteria; (2) occupational risks such as health workers or people with close contact with animals (farmers, veterinarians) who frequently use antibiotics; and (3) comorbidities. Eradication is rather difficult and, thus far, has not shown clear-cut results. Preventive measures will be important in the future with a reinforcement of hygienic measures not only in the hospital, but also in the community.
Antibiotic Prevalence Study and Factors Influencing Prescription of WHO Watch Category Antibiotic Ceftriaxone in a Tertiary Care Private Not for Profit Hospital in Uganda
Authors: Kizito Mark , Lalitha Rejani , Kajumbula Henry , Ssenyonga Ronald , Muyanja David , Byakika-Kibwika Pauline
Published: September 2021

Background: Excessive use of ceftriaxone contributes to the emergence and spread of antimicrobial resistance (AMR). In low and middle-income countries, antibiotics are overused but data on consumption are scarcely available. We aimed to determine the prevalence and factors influencing ceftriaxone prescription in a tertiary care private not-for-profit hospital in Uganda. Methods: A cross-sectional study was carried out from October 2019 through May 2020 at Mengo Hospital in Uganda. Patients admitted to the medical ward and who had been prescribed antibiotics were enrolled. Sociodemographic and clinical data were recorded in a structured questionnaire. Bivariate and adjusted logistic regression analyses were performed to determine factors associated with ceftriaxone prescription. Results: Study participants were mostly female (54.7%). The mean age was 56.2 years (SD: 21.42). The majority (187, 73.3%) presented with fever. Out of the 255 participants included in this study, 129 (50.6%) participants were prescribed ceftriaxone. Sixty-five (25.5%) and forty-one (16.0%) participants had a prescription of levofloxacin and metronidazole, respectively. Seven participants (2.7%) had a prescription of meropenem. Out of 129 ceftriaxone prescriptions, 31 (24.0%) were in combination with other antibiotics. Overall, broad-spectrum antibiotic prescriptions accounted for 216 (84.7%) of all prescriptions. Ceftriaxone was commonly prescribed for pneumonia (40/129, 31%) and sepsis (38/129, 29.5%). Dysuria [OR = 0.233, 95% CI (0.07–0.77), p = 0.017] and prophylactic indication [OR = 7.171, 95% CI (1.36–37.83), p = 0.020] were significantly associated with ceftriaxone prescription. Conclusions: Overall, we observed a high prevalence of prescriptions of ceftriaxone at the medical ward of Mengo Hospital. We recommend an antibiotic stewardship program (ASP) to monitor antibiotic prescription and sensitivity patterns in a bid to curb AMR.
Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy
Authors: Durán Fernández-Feijóo Cristina , Rodríguez-Fanjul Javier , Lopez-Abat Miriam , Hadley Stephanie , Cavia-Saiz Mónica , Muñiz Pilar , Arnaez Juan , Fernández-Lorenzo José Ramón , Camprubí Camprubí Marta
Published: September 2021

Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated, hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia + allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio and carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and cerebrospinal fluid, and 8-iso-prostaglandin F2α was measured in brain tissue. Plasma 2,2′–azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection in HIE.
The Cytotoxicity of Doxorubicin Can Be Accelerated by a Combination of Hyperthermia and 5-Aminolevulinic Acid
Authors: Kurokawa Hiromi , Matsui Hirofumi
Published: September 2021

Chemotherapy is cytotoxic to various cancer cells and as well as normal cells. Thus, treatments that demonstrate selective cytotoxicity for cancer cells are desired. The combination of chemotherapy and other cancer therapies can show synergic cytotoxicity, which may be a clue to the nature of the involved cancer cellar-specific damage. We previously reported a phenomenon whereby mitochondrial reactive oxygen species (mitROS) regulate the expression transporters involved in anticancer drug transport and mitROS production is increased by hyperthermia. Moreover, the uptake of 5-aminolevulinic acid (ALA) was enhanced by the increase in mitROS production. In this study, we investigated whether the combination of hyperthermia and ALA can enhance the cytotoxicity of doxorubicin. MitROS production and ALA-derived porphyrin accumulation by hyperthermia (HT) were increased in a murine breast cancer cell line. The expression of solute carrier 15A1 (SLC15A1) upregulated and an ATP-binding cassette subfamily G member 2 (ABCG2) downregulated by HT. Since SLC15A1 is an accumulating transporter for ALA, while ABCG2 is a porphyrin efflux transporter, porphyrin accumulation was enhanced. ABCG2 is also a doxorubicin efflux transporter. Thus, ALA treatment accelerates the intracellular concentration of porphyrin, which acts as a competitive inhibitor of doxorubicin. Indeed, the amount of intracellular doxorubicin was increased by a combination of HT and ALA. The cytotoxicity of doxorubicin was also enhanced. This enhancement was observed in the human breast cancer cell line while it was not seen in normal cells. The combination of HT and ALA treatment can enhance the cancer-specific cytotoxicity of doxorubicin.
BN-RA: A Hybrid Model for Risk Analysis of Overload-Induced Early Cable Fires
Authors: Chen Xiaolong , Huang Guozhong , Gao Xuehong , Ou Shengnan , Li Yatao , Hezam Ibrahim M.
Published: September 2021

Cable overload is one of the most critical contributors to early cable fires. This study proposes a hybrid Bayesian network (BN)-based fire risk analysis model, to investigate the evolution of overload-induced early cable fire risks. In particular, the fire risk transmission paths caused by cable overload are reported, considering the critical factors that likely lead to fires. A BN with a specific structure was considered using the fire risk transmission paths. Later, given the risk index system, a hybrid fire risk assessment model caused by cable overload was developed based on the entropy weight method. Subsequently, the corresponding risk levels were evaluated based on the evolution of the fire risk, using numerical simulations. Finally, a case study was conducted to validate the proposed methods, and the results indicated that the proposed methods can effectively evaluate the state of the cable and explain the causes of fire risk, which can be used for early fire warnings.
Concealed Object Detection and Recognition System Based on Millimeter Wave FMCW Radar
Authors: Liu Jie , Zhang Kai , Sun Zhenlin , Wu Qiang , He Wei , Wang Hao
Published: September 2021

At present, millimeter wave radar imaging technology has become a recognized human security solution in the field. The millimeter wave radar imaging system can be used to detect a concealed object; multiple-input multiple-output radar antennas and synthetic aperture radar techniques are used to obtain the raw data. The analytical Fourier transform algorithm is used for image reconstruction. When imaging a target at 90 mm from radar, which belongs to the near field imaging scene, the image resolution can reach 1.90 mm in X-direction and 1.73 mm in Y-direction. Since the error caused by the distance between radar and target will lead to noise, the original reconstruction image is processed by gamma transform, which eliminates image noise, then the image is enhanced by linearly stretched transform to improve visual recognition, which lays a good foundation for supervised learning. In order to flexibly deploy the machine learning algorithm in various application scenarios, ShuffleNetV2, MobileNetV3 and GhostNet representative of lightweight convolutional neural networks with redefined convolution, branch structure and optimized network layer structure are used to distinguish multi-category SAR images. Through the fusion of squeeze-and-excitation and the selective kernel attention mechanism, more precise features are extracted for classification, the proposed GhostNet_SEResNet56 can realize the best classification accuracy of SAR images within limited resources, which prediction accuracy is 98.18% and the number of parameters is 0.45 M.
Assessment of Laser Galvanometer Scanning System Accuracy Using Ball-Bar Standard
Authors: Kobiela Konrad , Jedynak Michał , Harmatys Wiktor , Krawczyk Marcin , Sładek Jerzy A.
Published: September 2021

The laser projector based on the laser galvanometer scanning system enables the projection of CAD-based laser images onto geometrical objects. Furthermore, the system can perform a scan of components in order to control proper positioning (e.g., welded structures). We decided to conduct research aimed at determining the suitability of such systems for length measurements and assess their accuracy. These systems are commonly calibrated with the use of flat calibration boards, although their capability of projecting and scanning 3D objects. For this reason, a new method based on ISO 10360 has been proposed. Analysis of the system’s ability to perform length measurements, selection of a reference standard, and the tested device accuracy were the main objectives of the study. A ball-bar with a nominal length of 3000 mm was chosen as a reference standard. Positions of the reference element in the workspace were determined, and three series of five measured deviations were performed in each setting. The obtained values of measurement errors prove that it is possible to use ball-bar standards to assess the accuracy of the described systems, which is defined by the equation MPE(E0) = ±0.5 mm. The proposed method could be adapted to perform more complex analyzes in this area.
Instantaneous Frequency Representation Used for CPA Laser Simulation
Authors: Oksenhendler Thomas , Bock Stefan , Schramm Ulrich
Published: September 2021

In the current study, we present a novel intuitive graphical method for the simulation of nonlinear effects on stretched pulses, characterized by a large time-bandwidth product. By way of example, this method allows precise determination of effects occurring in CPA (chirped pulse amplification) laser chains, such as the pre-pulse generation by the nonlinear Kerr effect. This method is not limited to first-order dispersion and can handle all resulting distortions of the generated pre-pulse.
Investigation of the Kinetic Regularities of the Process of Biodegradation of Betaine Surfactant by Bacteria of the Genus Pseudomonas
Authors: Burlachenko Anastasia S. , Salishcheva Olesya V. , Dyshlyuk Lyubov S. , Prosekov Alexander Y.
Published: September 2021

Surfactants are extremely common organic compounds that enter the environment in large quantities in the form of household and industrial wastewater. The toxicity of surfactants for biological systems, the high concentration of substances and the duration of the bioremediation process of polluted ecosystems requires improving the biotechnology of microbial wastewater treatment for surfactants. The purpose of this work is to study the kinetic laws of the reaction of the biological decomposition of betaine surfactants. Pseudomonas bacteria were used as bio-destructors of the surfactants. Kinetic data were obtained to create the possibility of further optimization of research on the biodegradation of toxic organic substances. The strains that were promising destructors of cocamidopropylbetaine were selected. The toxicity of high concentrations of surfactants in relation to microorganisms of the genus Pseudomonas was proven. Safe values of the surfactant concentration for conducting biodegradation tests were found. A kinetic model of the biodestructive process was constructed. It proves that the processes of biodegradation are described by a kinetic equation of the first order. With the derived equation, it is possible to determine the time interval of biodegradation of cocamidopropylbetaine to the specified values by means of mathematical calculations.
Aerial Tele-Manipulation with Passive Tool via Parallel Position/Force Control
Authors: Mohammadi Mostafa , Bicego Davide , Franchi Antonio , Barcelli Davide , Prattichizzo Domenico
Published: September 2021

This paper addresses the problem of unilateral contact interaction by an under-actuated quadrotor UAV equipped with a passive tool in a bilateral teleoperation scheme. To solve the challenging control problem of force regulation in contact interaction while maintaining flight stability and keeping the contact, we use a parallel position/force control method, commensurate to the system dynamics and constraints in which using the compliant structure of the end-effector the rotational degrees of freedom are also utilized to attain a broader range of feasible forces. In a bilateral teleoperation framework, the proposed control method regulates the aerial manipulator position in free flight and the applied force in contact interaction. On the master side, the human operator is provided with force haptic feedback to enhance his/her situational awareness. The validity of the theory and efficacy of the solution are shown by experimental results. This control architecture, integrated with a suitable perception/localization pipeline, could be used to perform outdoor aerial teleoperation tasks in hazardous and/or remote sites of interest.
Influence of Humic Acid on the Transport of Two Types of Synthesized Zinc Oxide Nanoparticles in Quartz Sand
Authors: Kim Donghyun , Hwang Gukhwa
Published: September 2021

In this study, transport and retention behaviors of the two types of nZnO prepared with separate manufacturing methods were compared/analyzed according to the presence/absence of Suwannee River humic acid (SRHA) adsorbed into the sand surface and the SRHA suspended in bulk solution, and to changes in the solution ionic strength (0.1–10 mM) in sand-repacked water-saturated columns. In the absence of suspended SRHA, nZnO-1 breakthrough was observed only in SRHA-coated soil, and the breakthrough amount decreased with the increase in the ionic strength (23.8% to 17.2% at 0.1 mM to 10 mM, respectively). In contrast, nZnO-2 breakthrough was not observed over the entire ionic strength range, regardless of the SRHA sand coating. With the presence of suspended SRHA, neither nZnO-1 nor nZnO-2 showed a significant difference in the breakthrough amount regardless of sand coating or ionic strength. However, the breakthrough amount of nZnO-1 was higher than that of nZnO-2 (51.5% versus 37.7% at 10 mM with 1 mg/L SRHA). From confirming the difference in transport between the two types of nZnO, the amount of SRHA adsorbed into nZnO-1 was less than the amount adsorbed into nZnO-2 (0.29 mg/g versus 0.64 mg/g at 10 mM with 1 mg/L SRHA). This result was considered to be due to the larger nZnO-1 breakthrough amount than the nZnO-2 breakthrough amount, which was caused by the larger amount of suspended SRHA that could occupy the deposition sites in the nZnO-1 suspension. Because the rate of SRHA deposition on the sand surface was higher than on nZnO, nZnO transport improved in the presence of suspended SRHA, and changed according to the amount of suspended SRHA.
The Impact of Effective Microorganisms on Flesh Color and Chemical Composition of Raw Potato Tubers
Authors: Pszczółkowski Piotr , Krochmal-Marczak Barbara , Sawicka Barbara , Pszczółkowski Mateusz
Published: September 2021

The objective of this study was to develop an innovative method of potato cultivation, with limited use of chemicals, for use in food processing. The results of the research are based on field experiments carried out between 2015 and 2017 at the Experimental Station in Parczew, Poland. The first-order factors were cultivation practices: A—standard cultivation practices with fungicides to control potato blight; and cultivation practices B, C, and D using the application of effective microorganisms (EM). The potato varieties were the second-order factor. The scope of the research included assessing the flesh color of the raw tubers and the content of dry mass, sugars and vitamin C. The color measurement of raw tubers was carried out using the CIEL *a *b* system. Cultivation methods using applications of effective microorganisms contributed significantly to the change in color brightness of the raw tuber flesh compared to the standard methods. The flesh color of the research potato cultivar tubers, their trichromatic coordinates and the reactions of potato varieties were determined according to the cultivation practices used. It was found that the brightness of the color of raw tuber flesh depends on the content of dry matter and the content of reducing sugars in the tubers.
Nonlinear Doubly Wiener Constant-Stress Accelerated Degradation Model Based on Uncertainties and Acceleration Factor Constant Principle
Authors: Wang Xiaoning , Su Xiaobao , Wang Jinjing
Published: September 2021

Although Wiener process models with the consideration of uncertainties, which are nonlinearity, random effects, and measurement errors, have been developed for lifetime prediction in the accelerated degradation test (ADT), they fail to describe the real degradation process because these models assume that the drift parameter correlates with the applied stress, while the diffusion parameter is constant. This paper put forward a nonlinear doubly Wiener constant-stress accelerated degradation model, where both diffusion and drift parameters were compatible with the applied stress according to the acceleration factor constant principle. When degradation data were available, we obtained the unknown parameters by applying a maximum likelihood estimation (MLE) algorithm in the constant-stress ADT (CSADT) model taking uncertainties into account. In addition, the proposed model’s effectiveness was validated through an illustrative example, and an application to the traveling wave tube (TWT) was carried out to demonstrate the superiority of our model in practical applications.
Apatite-Forming Ability of Flowable vs. Putty Formulations of Newly Developed Bioactive Glass-Containing Endodontic Cement
Authors: Edanami Naoki , Ibn Belal Razi Saifullah , Takenaka Shoji , Yoshiba Kunihiko , Yoshiba Nagako , Ohkura Naoto , Takahara Shintaro , Noiri Yuichiro
Published: September 2021

This study compared the apatite-forming ability (AFA) levels of flowable and putty formulations of Nishika Canal Sealer BG Multi (F-NBG and P-NBG, respectively) and attempted to clarify the cause of differences in the AFA levels of F-NBG and P-NBG. NBG samples were aged in simulated body fluid (SBF) or 1-, 5-, or 10-g/L bovine serum albumin-containing SBF (BSA-SBF) and analyzed in terms of their ultrastructures, elemental compositions, and Raman spectra to identify apatite formation. The phosphate ion consumption rates of NBG samples in the media were evaluated as an indicator of apatite growth. The original elemental composition, calcium ion release, and alkalizing ability levels of F-NBG and P-NBG were also evaluated. Apparent apatite formation was detected on all NBG samples except F-NBG aged in 10-g/L BSA-SBF. P-NBG consumed phosphate ions faster than F-NBG. As-prepared P-NBG showed more silicon elements on its surface than as-prepared F-NBG. P-NBG released more calcium ions than F-NBG, although their alkalizing ability levels did not differ statistically. In conclusion, the AFA of P-NBG was greater than that of F-NBG, probably because of the greater ability of P-NBG to expose silanol groups on the surface and release calcium ions.
Trigger-Based Dexterous Operation with Multimodal Sensors for Soft Robotic Hand
Authors: Huang Haiming , Lin Junhao , Wu Linyuan , Wen Zhenkun , Dong Mingjie
Published: September 2021

This paper focuses on how to improve the operation ability of a soft robotic hand (SRH). A trigger-based dexterous operation (TDO) strategy with multimodal sensors is proposed to perform autonomous choice operations. The multimodal sensors include optical-based fiber curvature sensor (OFCS), gas pressure sensor (GPS), capacitive pressure contact sensor (CPCS), and resistance pressure contact sensor (RPCS). The OFCS embedded in the soft finger and the GPS series connected in the gas channel are used to detect the curvature of the finger. The CPCS attached on the fingertip and the RPCS attached on the palm are employed to detect the touch force. The framework of TDO is divided into sensor detection and action operation. Hardware layer, information acquisition layer, and decision layer form the sensor detection module; action selection layer, actuator drive layer, and hardware layer constitute the action operation module. An autonomous choice decision unit is used to connect the sensor detecting module and action operation module. The experiment results reveal that the TDO algorithm is effective and feasible, and the actions of grasping plastic framework, pinching roller ball pen and screwdriver, and handshake are executed exactly.
Planning and Scheduling Optimization
Authors: Ouazene Yassine , Arbaoui Taha , Yalaoui Farouk
Published: September 2021

Optimizing the performance of services and industrial systems is a real lever for creating value for companies and society [...]
The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
Authors: Sheppard Colin J. R.
Published: September 2021

Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning of resolution and the basic principles and different approaches to superresolution are discussed.
Facilitators and Barriers in Applying Building Information Modeling (BIM) for Construction Industry
Authors: Shin Min-Ho , Kim Hwan-Yong
Published: September 2021

The authors provide a holistic perspective to enhance BIM working environment for more efficient smart construction settlements. An in-depth survey with 40 questionnaires was distributed to 100 professionals in construction, design, engineering, government. Although 45% of the respondents have received a certain form of BIM education, more than 94% of the respondents have answered that there should be a formal BIM education. Both design and construction experts have reported that the biggest advantage of using BIM is in its error checking ability. It can reduce design errors and increase construction ability. More than 79% of the respondents agreed upon a proper compensation for BIM implementation. The majority of opinions have revealed that there should be an increase in construction and design fees. There are a number issues for a successful BIM application: (1) guidelines and details for BIM work and delivery; (2) more standardized BIM education; and (3) a proper compensation system for BIM work. The results show that BIM professionals are ready to adopt the consequences of using BIM, but administrative and institutional preparations are not available beforehand. There should be a higher level of regulatory concern and provision of administrative solutions for successful BIM applications.
Deformation Behavior of Saturated Soft Clay under Cyclic Loading with Principal Stress Rotation
Authors: Fu Zunan , Wang Guoshuai , Song Wenbo , Yu Yanming , Wei Pengfei , Wu Tingyu
Published: September 2021

Under long-term traffic loading, the soil elements in subgrade are subjected to continuous principal stress rotation. In order to study the deformation properties of soft clays under traffic loading with principal stress rotation, a series of cyclic torsional shear tests were conducted on Wenzhou soft clays under different torsional cyclic stress ratios and degrees of principal stress rotation. The test results showed the stiffness softening of soil under long-term traffic loading. In addition, the principal stress rotation induced by traffic loading aggravated the deformation of clay samples and pore pressure accumulation. A modified dynamic pore pressure model was applied to consider the effect of principal stress rotation on undrained cumulative pore pressure, predicting the growth of cumulative pore pressure at different cycles. Considering loading cycles and the principal stress rotation, a modified Hardin–Drnevich (H-D) backbone curve model under traffic loading with principal stress rotation was proposed, and the predictive values of this model agreed well with the experimental values. Compared with the traditional H–D model, this model better reflects the cyclic deformation of soft clays under long-term traffic loading with principal stress rotation.
The Value of Contrast-Enhanced Ultrasound (CEUS) in Differentiating Testicular Masses: A Systematic Review and Meta-Analysis
Authors: Tufano Antonio , Flammia Rocco Simone , Antonelli Luca , Minelli Rocco , Franco Giorgio , Leonardo Costantino , Cantisani Vito
Published: September 2021

Ultrasound (US) still represents the mainstay of scrotal imaging. However, contrast-enhanced ultrasound (CEUS) is a relatively novel, but increasingly utilized diagnostic modality. In consequence, we performed a systematic review (SR) and pooled meta-analysis to investigate the diagnostic performance of CEUS in the evaluation of testicular masses (TM). A SR up to June 2021 was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The diagnostic performance of CEUS was evaluated basing on two different endpoints: neoplastic vs. non-neoplastic and malignant vs. benign TM. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) from eligible studies were pooled and summary receiver operating characteristic (SROC) curves were constructed for each endpoint. Overall, six qualified studies were deemed suitable for this meta-analysis. Diagnostic performance of CEUS showed an accuracy of 0.96 in detecting neoplastic masses (sensitivity of 0.89, PPV of 0.85, specificity of 0.62, and NPV of 0.69) and an accuracy of 0.96 in detecting malignant masses (sensitivity of 0.86, PPV of 0.73, specificity of 0.87, and NPV of 0.91). Taken together, CEUS may represent a promising minimally invasive diagnostic tool for characterization of TM, since it allows clinicians to identify neoplastic lesions and exclude malignant tumor.
Evaluation of Seasonal and Spatial Variations in Water Quality and Identification of Potential Sources of Pollution Using Multivariate Statistical Techniques for Lake Hawassa Watershed, Ethiopia
Authors: Lencha Semaria Moga , Ulsido Mihret Dananto , Muluneh Alemayehu
Published: September 2021

The magnitude of pollution in Lake Hawassa has been exacerbated by population growth and economic development in the city of Hawassa, which is hydrologically closed and retains pollutants entering it. This study was therefore aimed at examining seasonal and spatial variations in the water quality of Lake Hawassa Watershed (LHW) and identifying possible sources of pollution using multivariate statistical techniques. Water and effluent samples from LHW were collected monthly for analysis of 19 physicochemical parameters during dry and wet seasons at 19 monitoring stations. Multivariate statistical techniques (MVST) were used to investigate the influences of an anthropogenic intervention on the physicochemical characteristics of water quality at monitoring stations. Through cluster analysis (CA), all 19 monitoring stations were spatially grouped into two statistically significant clusters for the dry and wet seasons based on pollution index, which were designated as moderately polluted (MP) and highly polluted (HP). According to the study results, rivers and Lake Hawassa were moderately polluted (MP), while point sources (industry, hospitals and hotels) were found to be highly polluted (HP). Discriminant analysis (DA) was used to identify the most critical parameters to study the spatial variations, and seven significant parameters were extracted (electrical conductivity (EC), dissolved oxygen (DO), chemical oxygen demand (COD), total nitrogen (TN), total phosphorous (TP), sodium ion (Na+), and potassium ion (K+) with the spatial variance to distinguish the pollution condition of the groups obtained using CA. Principal component analysis (PCA) was used to qualitatively determine the potential sources contributing to LHW pollution. In addition, three factors determining pollution levels during the dry and wet season were identified to explain 70.5% and 72.5% of the total variance, respectively. Various sources of pollution are prevalent in the LHW, including urban runoff, industrial discharges, diffused sources from agricultural land use, and livestock. A correlation matrix with seasonal variations was prepared for both seasons using physicochemical parameters. In conclusion, effective management of point and non-point source pollution is imperative to improve domestic, industrial, livestock, and agricultural runoff to reduce pollutants entering the Lake. In this regard, proper municipal and industrial wastewater treatment should be complemented, especially, by stringent management that requires a comprehensive application of technologies such as fertilizer management, ecological ditches, constructed wetlands, and buffer strips. Furthermore, application of indigenous aeration practices such as the use of drop structures at critical locations would help improve water quality in the lake watershed.