bluuebunny/crossref_metadata_2025 huggingface dataset:
IL-4 and its polymorphism (IL4-589C/T) in cervical neoplasia
Authors: Abakumova T. V. , Myagdieva I. R. , Dolgova D. R. , Gening S. O. , Antoneeva I. I. , Gening T. P.
Published: June 2023

The transition of cervical neoplasia (CIN) to cervical cancer occurs with the active participation of IL-4, for which both pro- and antitumor effects have been shown with tumors of various localizations. The expression of cytokines is regulated at the transcriptional level in the promoter region of the gene. It has been shown that the genotype IL4 (589C/T) (rs2243250) is associated with the development of gastric and breast cancer. The contribution of IL-4 genotypic variations to the development of CIN has not yet been studied. The aim of the study was to assess the risk of developing cervical neoplasia by the presence polymorphism of IL4 (589C/T) and the level of IL-4. The object of the study was circulating neutrophils, serum and genomic DNA of 36 patients with CIN and 20 women without dysplasia (comparison group). Using ELISA, the level of IL-4 was determined in neutrophil lysate and serum. Phagocytic activity and adhesive ability (CD11b) of neutrophils were assessed. Allele-specific real-time PCR using Taq-Man probes was used to analyze of the IL4 589C/T (rs2243250). Statistical processing was carried out using Statistica 13 and Jamovi 1.6.5.0. As a result of the study, it was found that the level of IL-4 in serum and circulating neutrophils in patients with CIN is significantly higher than in the comparison group. The -589C* allele of the IL4 gene and the TT genotype are more common in the group with CIN (55.5%) than in the control (25%). At the same time, a direct relationship was established between the presence of polymorphism and increased adhesive ability and with indicators of the phagocytic number of circulating neutrophils. Analysis of the incidence of IL4 C589T by the «case-control» method showed that the chances of CIN formation in carriers of the -589C allele and the TT genotype were 3.75 (95% CI: 1.013 - 13.880, Chi-square = 4.161, p = 0.042). The -589C* allele and TT IL4 genotype, neutrophil and serum IL-4 levels are associated with HPV infection. Using a binary logistic regression model, we demonstrated the possibility of using IL-4 levels in circulating neutrophils and IL-4 gene polymorphism (589C/T) for the differential diagnosis of patients with CIN (χ2 = 15.6, p = 0.001). Significant significance for their combination was assessed by ROC-curve analysis (IL-4 in neutrophils; IL4 (-589С*), 75% probability. Thus, the IL4 (589C/T) is associated with the adhesive and phagocytic activity of circulating neutrophils. In HPV-infected patients, IL4 gene polymorphism (589C/T) can serve as a marker for early detection and prognosis of CIN.
Immunomodulating effects of antitumor drugs Bruton tyrosine kinase inhibitors and the possibility of their use in allergic and infectious diseases
Authors: Torshina Yu. S. , Serebryanaya N. B. , Glazanova T. V. , Mikhalyova M. A. , Voloshin S. V.
Published: June 2023

Bruton's tyrosine kinase (BTK) inhibitors represent a class of drugs that have demonstrated their efficacy and safety in patients with chronic lymphocytic leukemia and non-Hodgkin's lymphomas who were considered refractory to any previously used type of therapy. BTK plays a key role in all stages of B lymphocyte development, but in recent years, there have been data indicating that BTK is also involved in the activation of myeloid cells.

The aim of this study is to analyze and systematize all published materials on the immunomodulatory effects of BTK inhibitors (ibrutinib, acalabrutinib, etc.).

A systematic review of the scientific literature was performed using a step-by-step search process in electronic databases (PubMed, Web of Science, ScienceDirect, and Scopus). The following keywords were used in the database search: “CLL”, “BTK”, “ibrutinib”, “COVID-19”, “allergy”, “inflammation.” The search for studies was conducted from the time of the first BTK inhibitor drug (ibrutinib) appearance in 2009 until December 2022.

The results of the study on the influence of BTK inhibitors on the functional state of B and T lymphocytes, neutrophils, and monocytes/macrophages are presented. The immunomodulatory effects of ibrutinib on adaptive and innate immune system cells, including CD4+ and CD8+T lymphocytes and NK cells, are described. Since BTK inhibitors alter the functional activity of phagocytic cells and the ratio of T cell populations, there is a suggestion about the possibility of using these drugs for the treatment of other nosological forms, not only B cell malignancies, which is currently being studied in clinical trials. Data on the use of BTK inhibitors to combat hyperacute inflammation and to suppress allergic reactions, including anaphylaxis, are summarized. In addition, the expediency of short-term use of BTK inhibitors to reduce the risk of side effects during oral immunotherapy and for desensitization to drugs is discussed.

The presented data indicate that BTK inhibitors are promising drugs with immunomodulatory effects. However, BTK inhibitors need to increase selectivity to reduce off-target effects on other kinases.
Innate immune factor gene expression profiles in patients with atopic dermatitis
Authors: Bystritskaia E. P. , Murashkin N. N. , Olisova O. Yu. , Materikin A. I. , Potapova M. B. , Vinnitskaya A. B. , Upatova A. G.
Published: June 2023

Atopic dermatitis is a multifactorial genetically determined inflammatory skin disease characterized by itching, chronically relapsing dermatitis, age-related features of localization and morphology of lesions. The pathogenesis of atopic dermatitis is complex and includes epigenetic alterations, involved in the genomic adaptation, immune response reactions and dysfunction of the epithelial barrier that together trigger the development of atopic dermatitis. The aim of this study is to detect the expression level for IL4, IL13, IL33, TLR2, TLR9 genes in the biological materials of atopic patients.

The targeted genes for further expression evaluation were selected according to our previous findings on genome-wide methylation study. We detected the cascades with the differentially methylated genes that are most likely to take place in atopic dermatitis. Thus, we investigated expression levels for the IL4, IL13, IL33, TLR2, TLR9 genes in the skin, peripheral blood mononuclear cells and whole blood cells using RT-PCR on 55 pediatric patients and 26 healthy volunteers, and on 50 adult patients. Statistical analysis was performed with the use of Kruskal-Wallis H test and Mann-Whitney U test. Targeted expression analysis revealed that in the skin samples the expression of TLR9 and IL4 was 12 times significantly lower (p < 0.0001, p < 0.0005) in the lesional skin; and there was a 6-fold decrease in case of TLR2 (p < 0.01). The results for blood mononuclear cells differed and expression levels for most of the assessed targets were significantly higher before treatment. We have also found out that those differences were strongly pronounced especially in an elder age group (12-18 y.o.). Studying the IL33 gene expression in the whole blood samples of adults revealed that its level was significantly higher in case of patients with moderate form of AD. Besides, we concluded that locally in the affected skin inflammatory immune response may dominate; in the mononuclear cells Th2 immune response apparently takes place. New insights on immunological markers and links among them may shed a light on atopic dermatitis pathogenic mechanisms. The detected molecules could play role as potential therapeutic targets and form a management approach for patients with atopic dermatitis.
Immuno-inflammatory markers of syntropic cardiovascular diseases
Authors: Ganieva Sh. Sh. , Ergasheva M. U. , Panoev Kh. Sh.
Published: June 2023

Objective: To study the immuno-inflammatory markers of coronary heart disease, arterial hypertension and their combined course. The research work included 116 patients with cardiovascular disease of middle and old age. The average age of patients is 62.4±1.27. All patients were examined at the Bukhara branch of the Republican scientific and practical Center for emergency medical care. During the analysis, it was found that the level of systolic arterial blood pressure directly depends on the concentration of fibrinogen – r = 0.30 and oppositely depends on the concentration of procalcitonin (PCT) – r = -0.3 and IL-6 – r = -0.26. At the same time, a noticeable positive association of heart rate with creatinine in the blood – r = 0.35 was also revealed. The established connections show the contribution of inflammation syndrome (immune) in the progression of hypertension, or rather, indicators of the progression of hypertension in coronary heart disease are creatinine, fibrinogen, PCT and IL-6. Due to the high and noticeable correlation with the studied immuno-biochemical parameters of blood and functional parameters of the heart, IL-6 is a more informative indicator of the progression of coronary heart disease with the risk of complications and multiple organ failure. Thus, the established connections in our studies allow us to include the conclusion that, along with the above, risk factors for the development of rupture and/ or aneurysm of the aorta in CHD are an increase in the blood level of complement C3, IL-17 and PCT. Consequently, with an increase in the level of VEGF in the blood in CHD AS, the risk of an increase in the thickness of the LV in the diastole increases, and an increase in IL-6, IL-17A and urea in the blood shows a decrease in the thickness of the LV in the diastole, which makes it possible to differentiate the restrictive variant from the hypertrophic form in atypical angina. Due to the high and noticeable correlation with the studied immuno-biochemical parameters of blood and functional parameters of the heart, IL-6 is a more informative indicator of the progression of coronary artery disease with the risk of complications and multiple organ failure.
Myeloid-derived suppressor cells as biomarkers of the effectiveness of therapy with new biological agents in axial spondyloarthritis
Authors: Tyrinova T. V. , Morenkova A. Yu. , Fedorova A. V. , Tikhonova M. A. , Ilina N. A. , Chumasova O. A. , Sizikov A. E.
Published: June 2023

Innate immune cells, including myeloid cells — myeloid derived suppressor cells (MDSCs) — are supposed to play an important role in the pathogenesis of axial spondyloarthritis (AxSp). Myeloid derived suppressor cells represent a heterogeneous population of immature cells capable of suppressing innate and adaptive immune responses with the most pronounced suppressor activity against T cells. Biological disease-modifying antirheumatic drugs (bDMARDs) can reduce the clinical and laboratory disease activity, but their effectiveness varies widely in different patients with AxSp. The present study is aimed at studying MDSCs subpopulations and their suppressive function depending on the response to bDMARD therapy in AxSp. The study included AxSp patients with a disease duration of 16.5 years (median); HLA-B27 (+) status was detected in 79% of cases. All patients received bDMARDs at least the past 12 weeks, including TNF inhibitors (etanercept, certolizumab pegol, adalimumab, or golimumab) or IL-17 inhibitors (secukinumab, ixekizumab, or netakimab). Percentage of granulocytic MDSCs (G-MDSCs, Lin-HLA-DR-CD33+CD66b+), monocytic MDSCs (M-MDSCs, HLA-DRlow/-CD14+), MDSCs of early stage differentiation (E-MDSCs, Lin-HLA-DR- CD33+CD66b-), as well as intracellular expression of arginase-1 was assessed by flow cytometry. Frequency of circulating MDSC subpopulations of patients with a stable response to bDMARDs (responders) did not differ significantly compared to healthy donors. Patients not responding to bDMARDs therapy showed increased relative and absolute number of E-MDSCs compared to healthy donors (pU = 0.01 and pU = 0.02, respectively) and the responders (pU = 0.03 and pU = 0.07, respectively). Increased percentage of E-MDSCs was positively correlated to disease activity — ESR (Rs = 0.821; p = 0.023), CRP (Rs = 0.714; p = 0.07) and ASDASCRP (Rs = 0.829; p = 0.042) in the non-responder group. Responder patients exhibited no correlation between disease activity and circulating MDSCs. The suppressor potential of MDSCs was analyzed by the intracellular expression of arginase-1 molecule which is involved in the inhibition of T cell response. Patients with the stable response were characterized by increased expression of arginase-1 in E-MDSCs compared to donors (pU = 0.02). Non-responders did not demonstrate significant changes in Arg-1 expression, however, the percentage of arginase-1-expressing G-MDSCs was positively correlated to indexes ASDASESR (Rs = 0.857; p = 0.014) and BASDAI (Rs = 0.785; p = 0.036). Thus, E-MDSCs as well as arginase-1 expression in MDSCs may serve as biomarkers of effectiveness bDMARD therapy, and act as potential candidate predictors of response to therapy in AxSp.
Nuclear transcription factor kB (NF-kB) activity in lymphocyte populations in children with Wilson-Konovalov disease
Authors: Kurbatova O. V. , Petrichuk S. V. , Kuptsova D. G. , Movsisyan G. B. , Radygina T. V. , Komarova A. D. , Anushenko A. O. , Freidlin E. V. , Semikina E. L. , Potapov A. S. , Fisenko A. P.
Published: June 2023

Wilson's disease (WD) is a rare hereditary disease caused by a deficiency of the ATF7B transporter. The accumulation of copper can cause damage to organs and cells, mainly the liver. Copper exposure can modulate cytokine synthesis through molecular and cellular signaling pathways, including the nuclear transcription factor NF-kB pathway. NF-kB is the main regulator of inflammation and cell death, acts as a central link between liver damage, fibrosis and hepatocellular carcinoma. An excess of NF-kB-dependent cytokine response stimulates inflammatory reactions, but excessive inhibition of NF-kB can negatively affect the viability of hepatocytes. Method of flow cytometry with visualization — Amnis ImageStreamX allows to evaluate the activity of NF-kB (% of activated cells in cell populations). The aim: to evaluate the activity of NF-kB in lymphocyte populations in children with WD disease. Immunophenotyping of lymphocytes and assessment of the level of translocation of NF-kB were performed in 52 children with WD and in 25 children of comparison group. The mass concentration of copper in daily urine was determined by atomic absorption method using the AAnalyst 800 spectrometer. In children with WD, the content of cells with NF-kB translocation varied from 5 to 90% depending on the lymphocyte population; the highest level was detected in B cells — 57.5 (37-68) %. A significant difference in distributions of the number of cells with NF-kB translocation between WD and healthy children was shown (F-criterion, p < 0.01). In most cases, children with WD are characterized by a decrease in the activity of NF-kB in populations of B cells (in 43% of cases), T helper cells (48%), T cytotoxic (44%) and Th17 lymphocytes (41%). In children with WD, the concentration of copper varied from 9.7 to 2582 mcg/day, Me = 616 (210-1173). A direct relationship was obtained between the copper content in urine and the level of translocation of NF-kB in B lymphocytes, r = 0.34, p = 0.016. The activity of the NF-kB correlates with biochemical markers of the severity of liver damage (ALT, AST, GGT) and with copper content in urine. The study of the NF-kB signaling pathway seems promising for a better understanding of the pathogenetic mechanisms of the formation of inflammation and liver fibrosis in children with WD.
Quantification of C1 esterase inhibitor in human serum by enzyme-linked immunosorbent assay: Correlation with turbidimetric immunoassay
Authors: Gorbunov N. P. , Zhakhov A. V. , Gorbunova I. N. , Milichkina A. M. , Drozd I. V. , Gubanova A. V. , Danilova E. M. , Kuznecova R. N. , Savin T. V. , Burtseva A. G. , Pigareva N. V. , Ischenko A. M. , Totolian Areg A.
Published: June 2023

C1 inhibitor of serine proteases (C1-INH) performs a regulatory function in the complement system and vascular permeability. Deficiency of C1-INH leads to various forms of angioedema, including hereditary angioedema (HAE). The cause of HAE is a genetically determined violation of the synthesis of C1-INH. A decrease in the level of C1-INH to 50% relative to the norm leads to an increase in the production of bradykinin, which is the basis for the diagnosis of HAE. The development of affordable ELISA for the quantitative determination of C1-INH is a popular direction for clinicians. During the development of a new kit for quantitative determination of C1-INH, two mouse monoclonal antibodies (mAb) with different epitope specificities were obtained. On their basis, a sandwich-type ELISA was developed. The specificity of the obtained mAb's was confirmed using the medical device “Berinert”. To prepare calibrators, C1-INH was affinity purified from human blood plasma using a sorbent with immobilized mAbs. The identity of the C1-INH protein was confirmed by PAGE electrophoresis, immunoblotting, and mass spectrometry on MALDI-TOF/TOF UltrafleXtreme mass spectrometer. To assess the quality indicators of developed reagents kit, studies were carried out in accordance with GOST R 51352-2013 and TU 21.20.23-041-01967164-2022. Values of quality indicators: accuracy — 93.53%; measurement linearity interval — 22.00-176.07 ng/mL. Using the developed ELISA test system, we examined 28 blood sera from healthy donors and 7 blood sera from patients with confirmed HAE. In the same samples, the content of C1-INH was determined by turbidimetric method, using the "Diagnostic reagents for in vitro immunochemical studies of specific blood proteins. Model: C1-esterase inhibitor (C1 EsteraseInhibitor)" (Aptec, Belgium). The correlation coefficient was 0.94 (p < 0.05). It was found that the diagnostic sensitivity and specificity of the developed ELISA is 100%. As a result of the study, an original ELISA test system for the quantitative determination of C1-INH was developed "Reagent kit for enzyme-linked immunosorbent assay of human C1-inhibitor (C1-inh PS)".
Role of morphogenic proteins of the WNT signaling pathway in coronary artery disease
Authors: Snimshchikova I. A. , Plotnikova M. O.
Published: June 2023

In recent years, researchers’ attention has been directed to the WNT signaling pathway study, which regulates embryogenesis processes and is involved in pathological condition development. The role of morphogenic proteins of WNT signaling pathway in the cardiovascular pathology genesis is practically not clear. The research aim was a comprehensive study of the main proteins of WNT signaling pathway (β-catenin, sclerostin, GSK-3α, GSK-3β, WIF-1 and DVL-1) in the blood serum of 353 patients with coronary artery disease acute forms who were treated at the Orel regional vascular center from 2019 to 2021, and 50 healthy individuals. A comprehensive analysis included an assessment of clinical, laboratory and instrumental parameters in the framework of current clinical guidelines, as well as an immunological examination to determine the morphogenic proteins of WNT signaling by enzyme immunoassay. The results showed a wide variability in the values of morphogenic proteins of WNT signaling pathway in the patient’s blood serum. The levels of β-catenin, WIF-1 and DVL-1 significantly exceeded those obtained in healthy individuals, while the concentrations of sclerostin and GSK-3β did not differ significantly from them. The level of GSK-3α of patients was twice lower than in healthy individuals. The highest sclerostin concentrations were found in patients with existing calcification of the aortic valve leaflets and aortic walls. Acute coronary syndrome unfavorable course was observed in patients with both extremely high and extremely low WIF-1 levels. Significant correlations were established between the level of morphogenic proteins of WNT signaling pathway and lipid metabolism, as well as myocardial remodeling. The obtained data on changes in the protein production of WNT signaling pathway allow us to expand our understanding of the molecular aspects of the immunopathogenesis of myocardial remodeling in coronary artery disease, increase the predictive potential for cardiovascular disease diagnosis and determine the vector for further development of cardioimmunology determination.
T-lymphocytes of the 2nd type of the immune response and their role in enhancing inflammation during the professional activities of firefighters
Authors: Bychkova N. V. , Kalashnikova A. A. , Kalinina N. M.
Published: June 2023

T lymphocytes of the 2nd type of immune response contribute to the development and exacerbation of inflammation, mainly allergic. Increased inflammation with prolonged exposure to adverse factors during the work of firefighters can lead to the development of various diseases. Evaluation of the immunity of firefighters is important for the appointment of adequate treatment and prevention of infectious and allergic diseases. This paper aimed to analyze the indicators of immunity in employees of the state fire service of EMERCOM of Russia, depending on the age and intensity of the professional workload. The surveyed were men (n = 79), mean age 31 years, work experience from 1 to 22 years, with different workload intensity. In peripheral blood, flow cytometry (Navios, FC 500, Beckman Coulter) was used to evaluate subpopulations of monocytes, the relative number of T lymphocytes of the 2nd type of immune response CD3+CD294+. The concentration of total immunoglobulin E (Immulite) was determined. From nasal secretions, the content of secretory immunoglobulin A (Vector Best) was evaluated (n = 30). Statistical processing of the results was performed using the Statistica 12.0 package (StatSoft). An increase in the number of CD3+CD294+ cells was observed in 16.5%. A direct correlation was found between the number of T lymphocytes 2 and the age of the examined persons (р < 0.05). In the group of firefighters with a more intense workload, an increase in the number of CD3+CD294+ cells were 5 times higher (р < 0.05). Among patients who had any disease of the respiratory tract, an increase in this population was observed statistically significantly more often – in 26% of cases versus 11.5%. A strong direct correlation was found between the number of T lymphocytes 2 and the duration of smoking experience (р < 0.05). A direct correlation was established between the number of T lymphocytes 2 and the concentration of total IgE (р < 0.05). A decrease in secretory IgA in the secret from the nasal passages was observed in 23% of firefighters, in 13% of the examined, the indicator went beyond the upper limit of the reference interval. Significantly more often revealed the deviation of this indicator from the reference values in firefighters with a high workload. An increase in the subpopulation of classical monocytes was established in the group of those examined with a high number of CD3+CD294+ cells (p < 0.05). Thus, with an increase in the professional workload of firefighters in unfavorable conditions of service, inhibition of protection to infection and aggravation of damage to the respiratory tract with an increase in the 2nd type of immune response are noted. Evaluation of the number of type 2 T lymphocytes in peripheral blood will reveal a predisposition to the T2 profile of immune inflammation, which will contribute to a personalized approach to patient management.
Combate à deficiência de vitamina A: linhas de ação e perspectivas
Authors: Diniz Alcides da Silva
Published: June 2023

Resumo A hipovitaminose A é um dos mais importantes problemas nutricionais nos países em desenvolvimento, tanto em termos de magnitude e distribuição espacial, quanto de efeitos deletérios à saúde. Constitui a principal causa de cegueira prevenível na infância. Estudos ep idemiológicos têm demonstrado o papel crucial da vitamina A na redução da mortalidade infantil e materna; embora os efeitos sobre a morbidade sejam menos consistentes. A distribuição universal de megadoses de vitamina A tem sido a medida emergencial mais utilizada na prevenção e controle da carência. A fortificação, bem como, o estímulo à produção e ao consumo de alimentos fontes de vitamina A são ações a serem desenvolvidas a médio e longo prazos. Os principais desafios para o controle da carência de vitamina A, nas próximas décadas, devem estar consubstanciados em um programa alicerçado em três linhas básicas de ação: a complementação diagnóstica, a intervenção em larga escala e o monitoramento/avaliação das ações.
Constipação crônica: prevalência no ambulatório do Hospital Geral de Pediatria do Instituto Materno Infantil de Pernambuco
Authors: Souza Má rcio F. T. de , Silva Giselia A. P. da
Published: June 2023

Resumo Objetivos: determinar a prevalência de constipação crónica(C.C) na infância do Ambulatório do Instituto Materno Infantil de Pernambuco (IMIP). Métodos: realizou-se um estudo de corte transversal no Ambulatório do Hospital Geral de Pediatria do IMlp, durante o mês de outubro de 1997. As mães dos pacientes com idade de 1 mês a 14 anos, atendidos pela primeira vez, após a consulta com o médico assisten te, eram convidadas a participar da pesquisa. Após a explicação dos objetivos do estudo e a concordância verbal em participar, a entrevista era iniciada. Foi aplicado um formulário estruturado, previamente testado, que tinha por objetivo identificar os pacientes constipados. A constipação foi definida pela presença de pelo menos dois dos seguintes parâmetros: eliminação, dolorosa ou com esforço, de fezes de consistência aumentada, com freqüência inferior <l trés vezes por semana. Resultados: a prevalência de constipação intestinal crónica foi de 15,9%. No sexo masculino, a prevalência foi de 11,1 %, e de 20,1 %, no sexo feminino; segundo a faixa etária, a prevalência foi a seguinte: lactentes 15,3%, pré-escolares 17,3%, escolares 13,2 %, adolescentes 16,7%. Conclusões: conclui-se que a CC é um sintoma prevalente, com predomínio no sexo feminino e em pré-escolares. É necessário a busca ativa do sintoma quando do atendimento rotineiro das crianças, objetivando uma oríentação adequada e a redução da morbidade associada ao problema.
Risk factors for neonatal respiratory distress syndrome in severe preeclampsia
Authors: Amorim Melânia M. R. de , Faúndes Anibal , Santos Luiz Carlos
Published: June 2023

Abstract Objectives: to identify predictive factors of respiratory distress syndrome (RDS) in severe preeclampsia. Methods: a cross-sectional study was conducted based on the secondary analysis of a clinical trial about the use of corticosteroids to prevent RDS in severe preeclampsia. Newborn. infants with (n=66) and without RDS (n=134) were compared. Results: only fetal distress and very low birth weight were associated with increased risk of RDS, white corticosteroid therapy was significantly protective after multiple regression analysis. Conclusions: when deciding whether to interrup t a pregnancy in cases ofserious preeclampsia the risk of excessive prematurity has to be weighed up against the patient's exposure to fetal distress, and corticosteroid therapy should always be recommended.
The right of children and adolescents living with HIV to participate in health care
Authors: Guaranha Daniela Dal Forno Kinalski , Antunes Bibiana Sales , Motta Maria da Graça Corso da
Published: June 2023

Abstract Objective To understand how health professionals perceive the right to participation, that is, the voice of children and adolescents living with HIV in relation to their own health care. Method A qualitative study with a participatory approach that used the Sensitive Creative Method. The participants were 16 health professionals from three Specialized Care Services in southern Brazil. The data were submitted to French Discourse Analysis. Results Respect for the rights in the care context, specifically the right to participation and decision-making, is still incipient. Children and adolescents have a voice in tangential aspects of their own health care but in a passive way, being restricted to a clinical and medication use perspective. Conclusion and implications for the practice Respect for the right to participation constitutes a promising link in health care and needs to be incorporated into the professionals' daily practice.
STRESS IN THE ELDERLY IN THE CONTEXT OF THE COVID-19 PANDEMIC AND ITS ASSOCIATED FACTORS
Authors: Arpasi-Quispe Orfelina , Fernandes-Molocho Lili , Mocarro-Aguilar Maria Rosario , Díaz-Orihuela Maria Magdalena , Fhon Jack Roberto Silva
Published: June 2023

ABSTRACT Objective: To identify stress in the elderly living in the city of Lima and its association with demographic factors, infodemics, the presence of physical and psychological symptoms, and the use of illicit substances in the context of the Covid-19 pandemic. Method: Quantitative, transversal, and analytical study through the web-based survey with 384 elderly from the city of Lima - Peru between April and August 2021. The instruments of demographic profile, Perceived Stress Scale, and self-reported symptoms were used for the study. The Multiple logistic regression was used, considering the significance level of 5%. Results: 62% were women, and the age ranged between 60 and 95 years with a mean of 70.5. The stress average was 26.8 points. Stress was associated with fear of relatives dying and concern for the elderly. Conclusions: it is important for health professionals trained to identify changes in mood in the elderly and to create individualized care plans.
FACTORS ASSOCIATED WITH THERAPEUTIC NON-ADHERENCE AMONG HYPERTENSIVE INDIVIDUALS WHO SEEK EMERGENCY CARE
Authors: Ferreira Patrícia Chatalov , Teston Elen Ferraz , Carvalho Brígida Gimenez , Silva Joice Lourenço da , Reis Pamela dos , Rossi Robson Marcelo , Marcon Sonia Silva
Published: June 2023

ABSTRACT Objective: to analyze factors associated with therapeutic non-adherence among individuals with Arterial Hypertension who seek emergency care and/or assistance due to hypertensive complications. Method: this is a cross-sectional study conducted from December 2019 to October 2020 with 238 people living in a medium-sized municipality from southern Brazil using Morisky’s 8-Item Medication Adherence Scale. Multiple Logistic Regression was used in the analysis. Results: a total of 86 (36.1%) participants were considered as non-adherent. A higher change of non-adherence was observed in people younger than 60 years of age (Odds Ratio=2.04), who sought emergency services in the three years under study (Odds Ratio=5.08), and who had a bond with Primary Health Care professionals (Odds Ratio=1.96). Conclusion: acknowledging the factors associated with non-adherence to the therapy will allow professionals to conduct educational interventions and assist people with hypertension according to their needs, thus preventing/postponing complications.
ESTRESSE EM IDOSOS NO CONTEXTO DA PANDEMIA DA COVID-19 E SEUS FATORES ASSOCIADOS
Authors: Arpasi-Quispe Orfelina , Fernandes-Molocho Lili , Mocarro-Aguilar Maria Rosario , Díaz-Orihuela Maria Magdalena , Fhon Jack Roberto Silva
Published: June 2023

RESUMO: Objetivo: identificar o estresse nos idosos que vivem na cidade de Lima e sua associação com fatores demográficos, infodemias, a presença de sintomas físicos e psicológicos e o uso de substâncias ilícitas no contexto da pandemia de COVID-19. Método: estudo quantitativo, transversal e analítico através da pesquisa baseada na web com 384 idosos da cidade de Lima - Peru, entre abril e agosto de 2021. Os instrumentos de perfil demográfico, Escala de Estresse Percebido e sintomas autorrelatados foram utilizados para o estudo. A regressão logística múltipla foi utilizada, considerando o nível de significância de 5%. Resultados: 62% eram mulheres, e a idade variou entre 60 e 95 anos, com uma média de 70,5 anos. A média de estresse foi de 26,8 pontos. O estresse estava associado ao medo da morte de parentes e à preocupação com os idosos. Conclusões: é importante para profissionais de saúde treinados para identificar mudanças de humor nos idosos e criar planos de cuidados individualizados.
EDUCATIONAL VIDEO FOR SELF-CARE OF PATIENTS WITH INTESTINAL ELIMINATION STOMA
Authors: Moreira Bruna Camila Blans , Lima Fernando Conceição de , Silva Cassilene Oliveira da , Carvalho Dione Seabra de , Simor Alzinei , Santana Mary Elizabeth de , Nascimento Marcia Helena Machado
Published: June 2023

ABSTRACT Objective: to describe the construction of an educational technology to mediate the self-care of cancer patients with intestinal elimination stoma. Method: methodological research, conducted in two stages - situational diagnosis and integrative literature review, carried out in the oncology reference hospital in the city of Belém-Pará, Brazil. The collection was carried out in the period from January to November 2021, through semi-structured interviews, with 10 oncology patients with stomas and in electronic databases. They were analyzed from the perspective of content analysis of the thematic type and supported by the Iramuteq software. Results: from the corpus a tree of similarity emerged, in which the word “Bag” has greater centrality and strong connection with the words, “Care”, “Change”, “Plaque”, “Colostomy” and “Stoma”. Based on this context, an educational video technology was produced. Conclusion: the production of context-based technology favors the collection of social evidence for self-care with the peristomal skin.
Consumer Preference and Quality Characteristics of Different-category Eggs Sold at Supermarkets in Rio de Janeiro
Authors: SOARES Pollianna Luciene da Silva , ROCHA Brenda da Silva da , SANTOS Larissa , FAVACHO Jhenifer Sara Pinheiro , REIS Túlio Leite , CALIXTO Ligia Fátima Lima
Published: June 2023

ABSTRACT This study approached the consumer preference and characteristics of eggs from various categories sold at supermarkets in Rio de Janeiro. The study was performed in three phases. In the first one, 180 'caipira' eggs were randomly acquired from three supermarkets considering the consumers’ socioeconomic profile (middle, upper, and lower class). In the second phase, 456 eggs from conventional, free-range, 'caipira' and omega-3 enriched categories were obtained without considering the socioeconomic condition. After the acquisition, the eggs were taken for further analyses made the next day. The last phase was based on the perception and prevalence of consumers found in the second stage. This time, 443 consumers answered a Google Forms questionnaire. 'Caipira' eggs sold at supermarkets frequented by upper-class consumers maintained a better quality. When social class was discarded as a criterion, eggs from 'caipira', free-range, organic, and omega-3 enriched systems showed inferior quality compared to conventional eggs. The consumer preferences indicated that much misinformation regarding technical aspects of egg production persists, despite the increasing consumption of this protein source. In addition, the study revealed that many consumers still think there are hormone inputs in poultry farming, and they know very little about the production process or the differences among the egg categories for sale at Brazilian supermarkets.
Desenvolvimento de habilidades espaciais: atividades práticas utilizando jogos digitais com estudantes de arquitetura e urbanismo
Authors: Santos Carlos Eduardo da Rocha , Dias Maria Angela , Paula Frederico Braida Rodrigues de
Published: June 2023

Este artigo discute uma das possibilidades de mudanças no processo de ensino e aprendizado do pensamento geométrico face à apropriação exponencial da tecnologia digital. O objetivo da pesquisa foi desenvolver atividades focadas em discentes de primeiro período do curso de Arquitetura e Urbanismo, utilizando o jogo Minecraft. O artigo é resultado de uma pesquisa tanto bibliográfica quanto empírica, de uma experiência didática. Por meio de uma aplicação prática na Universidade Federal de Juiz de Fora, apontou-se que o uso dos jogos pode contribuir para o desenvolvimento das habilidades espaciais e o pensamento geométrico dos estudantes dos primeiros períodos do curso de Arquitetura e Urbanismo. Em um ambiente tridimensional simulado de jogo digital, os discentes são estimulados a utilizarem raciocínio geométrico para a composição de arquiteturas, associando o espaço às formas e racionalizando o processo compositivo arquitetônico.
Oferta e participação nas práticas corporais e atividades físicas na Atenção Primária: análise de 2014 a 2022
Authors: Carvalho Fabio Fortunato Brasil de , Guerra Paulo Henrique , Silva Debora Bernardo da , Vieira Leonardo Araújo
Published: June 2023

Objetivo: analisar a tendência temporal e distribuição macrorregional da oferta de atividades coletivas de práticas corporais e atividades físicas (PCAF) e do número de participantes na Atenção Primária (APS), desenvolvidas por todos os profissionais de saúde e por Profissionais de Educação Física (PEF). Métodos: estudo ecológico de série temporal, usando dados do Sistema de Informação em Saúde para a Atenção Básica (Sisab) entre 2014 e 2022, por meio da análise de regressão Joinpoint. Resultados: em âmbito nacional, identificou-se aumento do quantitativo de atividades coletivas de PCAF e de participantes na APS desenvolvidas por todos os profissionais de saúde e por PEF entre 2014 e 2019, seguido de uma redução de ambos em 2020. Em 2021 e 2022 ocorreu novo aumento. Conclusão: ainda que tenha ocorrido a pandemia da Covid-19, um cenário de excepcionalidade, foram identificadas tendências de aumento tanto na oferta de atividades coletivas de PCAF quanto de participantes.
COMPUTATIONAL MODELING FOR THE PERFORMANCE PREDICTION OF QUAD-SECTOR ROTARY AIR PREHEATER AND COMPARATIVE STUDY WITH TRI-SECTOR AND BI-SECTOR ROTARY AIR PREHEATERS
Authors: Padhi Manas Ranjan , Ghose Prakash
Published: June 2023

Rotary air preheaters are mainly used to improve the economy of a steam power plant by preheating the air with the help
of hot flue gases from the boiler. Modern steam power plants are preferring tri-sector and quad-sector rotary air preheaters over bi-sector due to their better performance and lower leakage. In the present work, thermal behavior of a quad-sector rotary air preheater is the focus using computational fluid dynamics. The porous media approach is used for numerical simulation assuming local thermal equilibrium condition between fluid and solid. The performance of the air preheater is accessed by effectiveness and pressure drop. Porosity is estimated for four different cell shapes, which are used in the rotary
preheater core for numerical investigation. The study reveals that the core consisting of a triangular cell has the highest effectiveness. The effect of porosity on performance is also investigated for various cell shapes. The effect of important operating parameters, such as rotor speed, aspect ratio, and mass flow rate of fluids on the effectiveness, as well as pressure drop is investigated for a quad-sector rotary air preheater. The study ends with a comparative performance analysis between bi-, tri-, and quad-sector rotary air preheaters. The performance of quad-sector air preheater is found to be better than other two preheaters in terms of effectiveness, whereas its pressure drop is maximum.
Effects of Adapted Sports on Quality of Life in People with Physical Disabilities: A Brief Review
Authors: Rešek Tajda , Kuzmič Blaž , Kozinc Žiga
Published: June 2023

Regular physical activity (PA) improves overall health, reduces the risk of many diseases, strengthens muscles and bones, lowers blood pressure, slows resting heart rate, increases lung capacity, and improves the immune system, balance, and coordination of movement in the general population and in people with physical disabilities. Adapted sports allows people who cannot otherwise participate in personal activities, to engage in regular PA. The literature collected in this brief review suggests that involvement in adapted sports improves several areas of quality of life for people with physical disabilities. The majority of people with physical disabilities have poorer general health, limited social integration, and lower quality of life. In our literature review, we found that participation in adapted sports is an effective way to improve different domains of quality of life in people with physical disabilities. PA and sport have been shown to be very important factors in maintaining general health, social inclusion, and psychological well-being.
Eski Van Şehri Kaya Çelebi Camisi Onarımı Hakkında Bir Araştırma
Authors: ÖZTÜRK Şahabettin
Published: June 2023

Kaya Çelebi Camii, Van Kalesi’nin güneyindeki Eski Van Şehri’nin güney surlarına yakın bir alanda yer almaktadır. 1660 yılında Kaya Çelebizade Koçi Bey tarafından cami inşaatına başlanmış, daha sonra Cem Dedeoğlu Mehmet Bey tarafından tamamlanmıştır. Cami kapısının kemer alınlığı üzerinde yer alan kitabenin 1915-18 Rus-Ermeni işgali sırasında söküldüğü rivayet edilir. XVII. yüzyıl Osmanlı dönem mimarisi ile inşa edilen yapı, merkezi tek kubbeli, son cemaat bölümü üzeri beş benzer kubbeyle örtülü, kuzeybatı beden duvarına bitişik kare kaideli minareden oluşmaktadır. Yapının kuzeybatı köşesindeki kare kaide üzeri silindirik gövdeli tek şerefeli minare yer almaktadır. İnşa edildiği dönemden 1915 yılına kadar kesintisiz olarak kullanılan cami, 1990 yılına kadar tahrip edildiği için kullanılamamıştır. Yapının beden duvarları, son cemaat bölümü, minare, mahfil bölümü ve iç mekânda doğanın olumsuz etkisi ve definecilerin tahribatı ile ciddi statik sorunlar oluşturmuştur. İlk olarak 1971 yılında başlatılan esaslı onarım çalışmaları günümüze kadar dört farklı tarihlerde Vakıflar Genel Müdürlüğü tarafından yapılmıştır. Yapı, 2011 yılında Van’da oluşan iki büyük depremde ağır hasarlı olarak etkilenmiştir. Yapılan onarımda caminin kubbe, beden duvarlarının bir bölümü, son cemaat bölümü ve minaresi yeniden yapılmıştır. Kaya Çelebi Camii’nin son onarım çalışmalarından sonra 2012-15 yılları arasında Bitlis Vakıflar Bölge Müdürlüğü tarafından tamamlanarak 2016 yılında ibadete açılmıştır. Kaya Çelebi Camii, iç ve dış mimari öğelerinden mihrap, giriş kapısı, minare ve pencerelerde bitkisel ve geometrik süslemeler kullanılmıştır. Cami beden duvarları, yığma dolgu yapım tekniği ile inşa edilmiştir. Beden duvarlarında dıştan yonu taş örgüsü, içten kaba yonu üzeri Horasan harç sıvalı dolgu tekniği kullanılarak inşa edilmiştir.
Professional training and the ethical treatment of written police news
Authors: Jara Cabanillas Víctor
Published: June 2023

The objective of this study is to determine the relationship between the professional training of journalists and the ethical treatment of police news in the newspapers Correo y Satélite of Trujillo. After the analysis of the published police news and the specialty courses of the School of Communication Sciences of the National University of Trujillo, it was found that there is a weak correlation between both variables. It was also determined that 40% of the police news published in the newspaper Correo y Satélite contains weaknesses within the ethical aspect, since they present frequent uses of sensationalist terms, manipulation of images, exaggeration of information, inaccurate words, among other cataloged factors. as negative within the ethical treatment of news production. Likewise, in this investigation it was also found that 73% of the police news published in the Satélite newspaper have limitations within the ethical aspect. Meanwhile, the same problem presents 6.7% of the police news published in the regional edition of Correo – La Libertad.
Fin de vida durante la pandemia. Perspectiva de los profesionales de la salud dedicados a la atención domiciliaria en cuidados paliativos en Colombia
Authors: León-Delgado Marta Ximena , Acero Angela , Buitrago-Reyes Lina Angélica , Rodríguez-Campos Luisa Fernanda , Pino-Pinzón Carmen Juliana , Consuegra-Pareja César , Cuenca-Anacona Linda Lorena , González-Salazar Laura Vanessa , Michelsen-Andrade Mariana
Published: June 2023

Introducción: la pandemia ocasionada por el virus COVID-19 ha tenido gran impacto a nivel social y sanitario. Dado el alto riesgo de contagio y de colapso del sistema de salud, se recurrió al aislamiento social, al empleo de elementos de protección personal y a un mayor uso de la telemedicina. Esto generó cambios en la dinámica de atención de los pacientes al final de la vida.
Metodología: este estudio transversal observacional identificó las experiencias del personal de atención domiciliaria al final de la vida y el impacto de la pandemia en el proceso de atención, así como en su calidad de vida y afecto. Se realizó una encuesta con la adaptación del cuestionario Care Of the Dying Evaluation (iCODE), se evaluó la calidad de vida con la pregunta 30 del EORTC QLQ y la evaluación del afecto con la escala PANAS.
Resultados: se caracterizó la experiencia en 131 pacientes fallecidos. Los profesionales refieren que hubo un adecuado esfuerzo terapéutico para el control del dolor, disnea y delirium. Adicionalmente, afirman que la pandemia obstaculizó el tratamiento en el 24 % de los pacientes. La calidad de vida de los profesionales obtuvo una media de 6 puntos y el afecto predominante en estos fue positivo.
Conclusiones: los profesionales de salud de programas de atención domiciliaria consideran que la pandemia afectó el tratamiento de casi un cuarto de los pacientes en fin de vida, sin embargo, resaltan que estos recibieron un cuidado y control sintomático satisfactorios. Adicionalmente, consideran tener una adecuada calidad de vida.
Virtuelle Methoden in der Produktionstechnik
Authors: Jaensch Florian , Klingel Lars , Fur Shan , Verl Alexander
Published: June 2023

Neue Entwicklungen und innovative Lösungen in der Produktionstechnik werden größtenteils in Form von Software realisiert. Um die damit einhergehende Komplexität zu beherrschen, werden simulationsbasierte Methoden eingesetzt. Eine etablierte Methode, um Steuerungssoftware realitätsnah zu testen und abzusichern ist die Virtuelle Inbetiebnahme. Die Anwendung virtueller Methoden endet heutzutage aber mit dem  Produktionsstart. In diesem Artikel werden die Potenziale der Virtuellen Inbetriebnahme als Bindeglied zwischen der virtuellen und realen Produktion aufgezeigt und die notwendigen Voraussetzungen werden diskutiert.
Erste Schritte in ein Industrial Metaverse für Prozessanlagen
Authors: Labisch Daniel , Hutter Andreas , MacWilliams Asa , Kokkelink Bernd
Published: June 2023

Auch wenn die Möglichkeiten im Industrial Metaverse noch nach Zukunftsmusik klingen, beeindrucken die heute schon realisierbaren Lösungen. Dieser Beitrag fokussiert sich auf Anwendungen und Use Cases in Augmented Reality (AR), einem wesentlichen Baustein des Industrial Metaverse. Dazu werden in AR verfügbare Informationen vorgestellt, die in mehreren Use Cases genutzt werden. Zwei  beispielhafte Proof-of-Concepts veranschaulichen was bereits heute möglich ist.