bluuebunny/crossref_metadata_2025 huggingface dataset:
Basic Exploratory Study of Bisphenol A (BPA) Dietary Administration to Istrian Pramenka Rams and Male Toxicity Investigation
Authors: Šturm Sabina , Weber Klaus , Klinc Primož , Spörndly-Nees Ellinor , Fakhrzadeh Azadeh , Knific Tanja , Škibin Andrej , Fialová Věra , Okazaki Yoshimasa , Razinger Tanja , Laufs Jürgen , Kreutzer Robert , Pogačnik Milan , Švara Tanja , Cerkvenik-Flajs Vesna
Published: May 2022

Bisphenol A (BPA), an endocrine-disrupting chemical and environmental pollutant, has been reported by many researchers to induce male reproductive toxicity in different experimental models. In this study, we investigated whether long-term exposure for two months to 25 µg/kg body weight (low dose) of BPA affects spermatogenesis or sperm quality in young Istrian Pramenka rams exposed via diet. We evaluated body and testicular weights, histopathology of testes and epididymides, and sperm analyses, and compared these parameters between the group of treated rams and the control group of rams. Although there were some differences between the two groups, these differences were not large or statistically significant. The only statistically significant difference was the lower epithelial height of seminiferous tubules in treated rams, compared to control rams. In addition to assessing toxicity, BPA concentrations in the blood plasma of treated rams were determined after the first administration, and the toxicokinetic parameters of total BPA were calculated. In this study, no major signs of altered reproduction in rams were detected.
Correction: Pérez et al. Use of Nanotechnology to Mitigate Biofouling in Stainless Steel Devices Used in Food Processing, Healthcare, and Marine Environments. Toxics 2022, 10, 35
Authors: Pérez Hugo , Vargas Gregorio , Silva Rodolfo
Published: May 2022

The authors would like to make corrections to their published paper [...]
Combined Process of Biogenic Manganese Oxide and Manganese-Oxidizing Microalgae for Improved Diclofenac Removal Performance: Two Different Kinds of Synergistic Effects
Authors: Wang Quanfeng , Liao Cenhui , Zhao Jujiao , Zeng Guoming , Liu Wenbo , Gao Pei , Sun Da , Du Juan
Published: May 2022

Biogenic manganese oxides (Bio-MnOx) have attracted considerable attention for removing pharmaceutical contaminants (PhCs) due to their high oxidation capacity and environmental friendliness. Mn-oxidizing microalgae (MnOMs) generate Bio-MnOx with low energy and organic nutrients input and degrade PhCs. The combined process of MnOMs and Bio-MnOx exhibits good prospects for PhCs removal. However, the synergistic effects of MnOMs and Bio-MnOx in PhCs removal are still unclear. The performance of MnOMs/Bio-MnOx towards diclofenac (DCF) removal was evaluated, and the mechanism was revealed. Our results showed that the Bio-MnOx produced by MnOMs were amorphous nanoparticles, and these MnOMs have a good Mn2+ tolerance and oxidation efficiency (80–90%) when the Mn2+ concentration is below 1.00 mmol/L. MnOMs/Bio-MnOx significantly promotes DCF (1 mg/L) removal rate between 0.167 ± 0.008 mg/L·d (by MnOMs alone) and 0.125 ± 0.024 mg/L·d (by Bio-MnOx alone) to 0.250 ± 0.016 mg/L·d. The superior performance of MnOMs/Bio-MnOx could be attributed to the continuous Bio-MnOx regeneration and the sharing of DCF degradation intermediates between Bio-MnOx and MnOMs. Additionally, the pathways of DCF degradation by Bio-MnOx and MnOMs were proposed. This work could shed light on the synergistic effects of MnOMs and Bio-MnOx in PhCs removal and guide the development of MnOMs/Bio-MnOx processes for removing DCF or other PhCs from wastewater.
IVIVE: Facilitating the Use of In Vitro Toxicity Data in Risk Assessment and Decision Making
Authors: Chang Xiaoqing , Tan Yu-Mei , Allen David G. , Bell Shannon , Brown Paul C. , Browning Lauren , Ceger Patricia , Gearhart Jeffery , Hakkinen Pertti J. , Kabadi Shruti V. , Kleinstreuer Nicole C. , Lumen Annie , Matheson Joanna , Paini Alicia , Pangburn Heather A. , Petersen Elijah J. , Reinke Emily N. , Ribeiro Alexandre J. S. , Sipes Nisha , Sweeney Lisa M. , Wambaugh John F. , Wange Ronald , Wetmore Barbara A. , Mumtaz Moiz
Published: May 2022

During the past few decades, the science of toxicology has been undergoing a transformation from observational to predictive science. New approach methodologies (NAMs), including in vitro assays, in silico models, read-across, and in vitro to in vivo extrapolation (IVIVE), are being developed to reduce, refine, or replace whole animal testing, encouraging the judicious use of time and resources. Some of these methods have advanced past the exploratory research stage and are beginning to gain acceptance for the risk assessment of chemicals. A review of the recent literature reveals a burst of IVIVE publications over the past decade. In this review, we propose operational definitions for IVIVE, present literature examples for several common toxicity endpoints, and highlight their implications in decision-making processes across various federal agencies, as well as international organizations, including those in the European Union (EU). The current challenges and future needs are also summarized for IVIVE. In addition to refining and reducing the number of animals in traditional toxicity testing protocols and being used for prioritizing chemical testing, the goal to use IVIVE to facilitate the replacement of animal models can be achieved through their continued evolution and development, including a strategic plan to qualify IVIVE methods for regulatory acceptance.
Psychosocial Burden of Itch among Renal Transplant Recipients
Authors: Krajewski Piotr K. , Tyczyńska Kinga , Bardowska Klaudia , Olczyk Piotr , Krajewska Magdalena , Szepietowski Jacek C.
Published: May 2022

Itch is the most common symptom of chronic dermatoses. Moreover, itch may be associated with systemic disorders. Chronic kidney disease—associated itch (CKD-aI) may affect up to 20% of renal transplant recipients (RTR). The aim of the study was to assess psychosocial burden of itch in RTR. The study was performed on a group of 129 RTR, out of which 54 (41.9%) experienced itch in the previous 3 days. A specially designed questionnaire assessing anxiety, depression, stigmatization, and quality of life was used. Results: Patients suffering from itch in the previous 3 days achieved significantly higher scores in GAD-7 (p < 0.001), BDI (p < 0.001), HADS total score (p < 0.001), HADS Depression (p = 0.004), and HADS Anxiety (p < 0.001). Severity of itch correlated positively with HADS, stigmatization scale, and GAD-7. Itch in RTR was associated with higher incidence of depression assessed with BDI (OR 3.7). Moreover, higher prevalence of anxiety was found among patients suffering from CKD-aI, assessed with HADS A and GAD-7 (OR 2.7 and OR 4.8, respectively) The results of our study clearly demonstrate that itch among RTR is a significant burden. Higher prevalence of depression and anxiety in this groups indicate the necessity of addressing itch relief as a part of holistic approach to patients after renal transplantation.
Assessment of Fusarium-Damaged Kernels in Common Wheat in Romania in the Years 2015 and 2016 with Extreme Weather Events
Authors: Gagiu Valeria , Mateescu Elena , Belc Nastasia , Oprea Oana-Alexandra , Pîrvu Gina-Pușa
Published: May 2022

This article assesses the occurrence of Fusarium-damaged kernels (FDKs) in common wheat (Triticum aestivum) under the influence of environmental factors and extreme weather events in Romania (exceptionally high air temperatures and extreme pedological drought produced by a dipole block in summer 2015, and extreme precipitation and floods produced by an omega block in spring 2016). Wheat samples (N = 272) were analyzed for FDKs via visual estimation and manual weighing according to ISO 7970 and are statistically evaluated using SPSS. The dipole block in 2015 reduced the effects of environmental factors to non-significant correlations with FDKs, while the omega block in 2016 was non-significantly to very significantly correlated with FDKs in the northwestern and western regions. The occurrence of FDKs was favored for wheat cultivation in acidic soils and inhibited in alkaline soils. Wheat samples with FDKs ≥ 1% were sampled from crops grown in river meadows with high and very high risks of flooding. Knowing the contaminants’ geographical and spatial distributions under the influence of regular and extreme weather events is important for establishing measures to mitigate the effects of climate change and to ensure human and animal health.
An Update on the Cosmetic Use of Botulinum Toxin: The Pattern of Practice among Korean Dermatologists
Authors: Rho Nark-Kyoung , Han Kwang-Ho , Kim Hei-Sung
Published: May 2022

The efficacy and safety of botulinum toxin injection have made it a popular aesthetic procedure worldwide. A cross-sectional survey was performed in order to determine the pattern of type A botulinum toxin injections in cosmetic practice, for which an 18-item questionnaire was distributed to dermatologists. A total of 469 Korean board-certified dermatologists participated in the survey, with the following results: the main candidates for type A botulinum toxin injection were individuals in their 40–50 years (46.1%), followed by those in their 20–30 years (33.4%), and people over 60 years of age (20.5%). Overall, the upper face (the glabella, forehead, and crow’s line, in decreasing order) was the most favored area of injection (51%). In contrast, body contouring (i.e., shoulder, calf) and treatment for benign masseter hypertrophy were significantly more popular in the 20–30 years age group than their older counterparts. For wrinkle effacement, the most preferred dilution was 100 units/2.5 mL with isotonic sodium chloride injection (51.2%), and the most often used interval was six months (43.6%). About half (46.3%) of the dermatologists reported the experience of clinical cases which were suspicious of botulinum toxin resistance. Despite this, regarding the choice of the product, type A botulinum toxin products with greater cost-effectiveness were favored over products with a lower risk of antibody formation. Other than its cosmetic usage, botulinum toxin is applied for a variety of skin conditions. Further studies are suggested in order to identify the practice pattern of type A botulinum toxin for therapeutic uses in dermatology, such as hyperhidrosis and rosacea.
Innate and Adaptive Immune Responses in the Upper Respiratory Tract and the Infectivity of SARS-CoV-2
Authors: Ramasamy Ranjan
Published: May 2022

Increasing evidence shows the nasal epithelium to be the initial site of SARS-CoV-2 infection, and that early and effective immune responses in the upper respiratory tract (URT) limit and eliminate the infection in the URT, thereby preventing infection of the lower respiratory tract and the development of severe COVID-19. SARS-CoV-2 interferes with innate immunity signaling and evolves mutants that can reduce antibody-mediated immunity in the URT. Recent genetic and immunological advances in understanding innate immunity to SARS-CoV-2 in the URT, and the ability of prior infections as well as currently available injectable and potential intranasal COVID-19 vaccines to generate anamnestic adaptive immunity in the URT, are reviewed. It is suggested that the more detailed investigation of URT immune responses to all types of COVID-19 vaccines, and the development of safe and effective COVID-19 vaccines for intranasal administration, are important needs.
Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
Authors: Zhou Jianwei , Wang Yongxia , Zhou Linyi , Qiu Yonghui , Zhao Jie , Dai Beining , Feng Xufei , Hou Lei , Liu Jue
Published: May 2022

An extensive understanding of the interactions between host cellular and viral proteins provides clues for studying novel antiviral strategies. Porcine circovirus type 3 (PCV3) and type 4 (PCV4) have recently been identified as viruses that can potentially damage the swine industry. Herein, 401 putative PCV3 Cap-binding and 484 putative PCV4 Cap-binding proteins were characterized using co-immunoprecipitation and liquid chromatography-mass spectrometry. Both PCV3 and PCV4 Caps shared 278 identical interacting proteins, but some putative interacting proteins (123 for PCV3 Cap and 206 for PCV4 Cap) differed. A protein–protein interaction network was constructed, and according to gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analyses, both PCV3 Cap- and PCV4 Cap-binding proteins participated mainly in ribosome biogenesis, nucleic acid binding, and ATP-dependent RNA helicase activities. Verification assays of eight putative interacting proteins indicated that nucleophosmin-1, nucleolin, DEAD-box RNA helicase 21, heterogeneous nuclear ribonucleoprotein A2/B1, YTH N6-methyladenosine RNA binding protein 1, and Y-box binding protein 1 bound directly to both PCV3 and PCV4 Caps, but ring finger protein 2 and signal transducer and activator of transcription 6 did not. Therefore, the interaction network provided helpful information to support further research into the underlying mechanisms of PCV3 and PCV4 infection.
Mitigating the Impact of Emerging Animal Infectious Disease Threats: First Emerging Animal Infectious Diseases Conference (EAIDC) Report
Authors: Kuchipudi Suresh V. , Behring Deanna , Nissly Ruth , Chothe Shubhada K. , Gontu Abhinay , Ravichandran Abirami , Butler Ty
Published: May 2022

From 29 November to 1 December 2021, an “emerging animal infectious disease conference (EAIDC)” was held at the Pennsylvania State University. This conference brought together distinguished thought leaders in animal health, veterinary diagnostics, epidemiology and disease surveillance, and agricultural economics. The conference’s primary objective was to review the lessons learned from past experiences in dealing with high-consequence animal infectious diseases to inform an action plan to prepare for future epizootics and panzootics. Invited speakers and panel members comprised world-leading experts in animal infectious diseases from federal state agencies, academia, professional societies, and the private sector. The conference concluded that the biosecurity of livestock operations is critical for minimizing the devastating impact of emerging animal infectious diseases. The panel also highlighted the need to develop and benchmark cutting-edge diagnostics for rapidly detecting pathogens in clinical samples and the environment. Developing next-generation pathogen agnostic diagnostics will help detect variants of known pathogens and unknown novel pathogens. The conference also highlighted the importance of the One Health approach in dealing with emerging animal and human infectious diseases. The recommendations of the conference may be used to inform policy discussions focused on developing strategies for monitoring and preventing emerging infectious disease threats to the livestock industry.
Identification of Inhibitors and Drug Targets for Human Adenovirus Infections
Authors: Liu Minli , Jiang Lefang , Cao Weihua , Wu Jianguo , Chen Xulin
Published: May 2022

Adenoviruses can cause infections in people of all ages at all seasons of the year. Adenovirus infections cause mild to severe illnesses. Children, immunocompromised patients, or those with existing respiratory or cardiac disease are at higher risk. Unfortunately, there are no commercial drugs or vaccines available on the market for adenovirus infections. Therefore, there is an urgent need to discover new antiviral drugs or drug targets for adenovirus infections. To identify potential antiviral agents for adenovirus infections, we screened a drug library containing 2138 compounds, most of which are drugs with known targets and past phase I clinical trials. On a cell-based assay, we identified 131 hits that inhibit adenoviruses type 3 and 5. A secondary screen confirmed the antiviral effects of 59 inhibitors that inhibit the replication of adenoviruses type 3 or 5. Most of the inhibitors target heat shock protein, protein tyrosine kinase, the mTOR signaling pathway, and other host factors, suggesting that these host factors may be essential for replicating adenoviruses. Through this study, the newly identified adenovirus inhibitors may provide a start point for developing new antiviral drugs to treat adenovirus infections. Further validation of the identified drug targets can help the development of new therapeutics against adenovirus infections.
The Effect of Waning on Antibody Levels and Memory B Cell Recall following SARS-CoV-2 Infection or Vaccination
Authors: Forgacs David , Silva-Moraes Vanessa , Sautto Giuseppe A. , Hanley Hannah B. , Gattiker Jasper L. , Jefferson Alexandria M. , Kolhe Ravindra , Ross Ted M.
Published: May 2022

In order to longitudinally track SARS-CoV-2 antibody levels after vaccination or infection, we assessed anti-RBD antibody levels in over 1000 people and found no significant decrease in antibody levels during the first 14 months after infection in unvaccinated participants, however, a significant waning of antibody levels was observed following vaccination. Participants who were pre-immune to SARS-CoV-2 prior to vaccination seroconverted to higher antibody levels, which were maintained at higher levels than in previously infected, unvaccinated participants. Older participants exhibited lower level of antibodies after vaccination, but a higher level after infection than younger people. The rate of antibody waning was not affected by pre-immunity or age. Participants who received a third dose of an mRNA vaccine not only increased their antibody levels ~14-fold, but also had ~3 times more antibodies compared to when they received their primary vaccine series. PBMC-derived memory B cells from 13 participants who lost all circulating antibodies were differentiated into antibody secreting cells (ASCs). There was a significant recall of memory B cell ASCs in the absence of serum antibodies in 5–8 of the 10 vaccinated participants, but not in any of the 3 infected participants, suggesting a strong connection between antibody levels and the effectiveness of memory B cell recall.
Oral Immunization of Chickens with Probiotic Lactobacillus crispatus Constitutively Expressing the α-β2-ε-β1 Toxoids to Induce Protective Immunity
Authors: Khan Mohammad , Li Fengsai , Huang Xuewei , Nouman Muhammad , Bibi Roshna , Fan Xiaolong , Zhou Han , Shan Zhifu , Wang Li , Jiang Yanping , Cui Wen , Qiao Xinyuan , Li Yijing , Wang Xiaona , Tang Lijie
Published: May 2022

Clostridium perfringens (C. perfringens) is a bacterium that commonly causes zoonotic disease. The pathogenicity of C. perfringens is a result of the combined action of α, β, and ε exotoxins. In this study, Lactobacillus crispatus (pPG-T7g10/L. crispatus) expressing the main toxoids of C. perfringens, α, ε, β1, and β2, with EGFP-labeling, was constructed, and the protective effect was estimated in chickens. The α-β2-ε-β1 toxoid was constitutively expressed for confirmation by laser confocal microscopy and western blotting, and its immunogenicity was analyzed by enzyme-linked immunosorbent assay (ELISA) and immunohistochemical assays. After booster immunization, the probiotic vaccine group showed significantly higher levels (p < 0.05) of specific secretory IgA (sIgA) and IgY antibodies in the serum and intestinal mucus. Furthermore, the levels of cytokines, including interferon (IFN)-γ, interleukin (lL)-2, IL-4, IL-10, IL-12, and IL-17, and the proliferation of spleen lymphocytes in chickens orally immunized with pPG-E-α-β2-ε-β1/L. crispatus increased significantly. Histopathological observations showed that the intestinal pathological changes in chickens immunized with pPG-E-α-β2ε-β1/L. crispatus were significantly alleviated. These data reveal that the probiotic vaccine could stimulate mucosal, cellular, and humoral immunity and provide an active defense against the toxins of C. perfringens, suggesting a promising candidate for oral vaccines against C. perfringens.
Evaluation of the Neutralizing Antibodies Response against 14 SARS-CoV-2 Variants in BNT162b2 Vaccinated Naïve and COVID-19 Positive Healthcare Workers from a Northern Italian Hospital
Authors: Sammartino Josè Camilla , Cassaniti Irene , Ferrari Alessandro , Giardina Federica , Ferrari Guglielmo , Zavaglio Federica , Paolucci Stefania , Lilleri Daniele , Piralla Antonio , Baldanti Fausto , Percivalle Elena
Published: May 2022

SARS-CoV-2 still represents a global health burden, causing more than six million deaths worldwide. Moreover, the emergence of new variants has posed new issues in terms of vaccine efficacy and immunogenicity. In this study, we aimed to evaluate the neutralizing antibody response against SARS-CoV-2 variants in different cohorts of vaccinated and unvaccinated subjects. Four-fold diluted sera from SARS-CoV-2 naïve and recovered subjects vaccinated with two or three doses of the BNT162b2 vaccine were challenged against 14 SARS-CoV-2 variants, and the SARS-CoV-2 neutralizing antibody titer was measured. Results were compared with those obtained from unvaccinated COVID-19 recovered patients. Overall, a better SARS-CoV-2 NT Abs response was observed in recovered vaccinated subjects after three doses of the vaccine when compared to unvaccinated patients and vaccinated subjects with only two doses. Additionally, the lowest level of response was observed against the Omicron variant. In conclusion, third doses of BNT162b2 vaccine seems to elicit a sustained response against the large majority of variants.
Perceptions and Knowledge towards COVID-19 Vaccine Hesitancy among a Subpopulation of Adults in Kenya: An English Survey at Six Healthcare Facilities
Authors: Shah Jasmit , Abeid Abdulaziz , Sharma Karishma , Manji Soraiya , Nambafu Jamila , Korom Robert , Patel Keya , Said Mohamed , Mohamed Mohamed Ali , Sood Mohamed , Karani Victor , Kamandi Patrick , Kiptinness Sarah , Rego Ryan T. , Patel Rajiv , Shah Reena , Talib Zohray , Ali Sayed K.
Published: May 2022

Background: Vaccine hesitancy, as defined by the WHO, is the reluctance or refusal to vaccinate despite the availability of vaccines and is one of the ten threats to global health in 2019. Vaccine hesitancy remains a complex matter influenced by multiple factors, especially in sub-Saharan Africa. Methods: We conducted a cross-sectional study between November 2021 and January 2022 among the general adult public seeking care at six different healthcare facilities in Kenya. The survey, in English, consisted of questions based on demographics, knowledge, and attitudes, including hesitancy towards the COVID-19 vaccine. Results: Of the 3996 surveys collected, 55.1% were from private, 19.5% from faith-based and 25.3% from government facilities., Approximately 81.0% of all the participants reported it was important to get a vaccine to protect other people from COVID-19, 79.9% reported they would take a vaccine to protect against COVID-19, yet 40.5% reported being hesitant to take the vaccine primarily due to side effects. Most of the variables were associated with receiving a vaccine. Only 52.1% of those seeking care from the government facility and 54.5% of those seeking care from the faith-based facility were vaccinated, compared to 81.5% seeking care from the private facilities (p < 0.001). More participants from private facilities felt that vaccines are safe as compared to those at the faith-based and government facilities (p < 0.001). Conclusion: Vaccine hesitancy in Kenya, even though much lower than reported in other countries, remains a dynamic problem. Mitigating strategies specific to Africa need to be developed to help address vaccine hesitancy in this part of the continent.
Could SARS-CoV-2 Have Bacteriophage Behavior or Induce the Activity of Other Bacteriophages?
Authors: Brogna Carlo , Brogna Barbara , Bisaccia Domenico Rocco , Lauritano Francesco , Marino Giuliano , Montano Luigi , Cristoni Simone , Prisco Marina , Piscopo Marina
Published: May 2022

SARS-CoV-2 has become one of the most studied viruses of the last century. It was assumed that the only possible host for these types of viruses was mammalian eukaryotic cells. Our recent studies show that microorganisms in the human gastrointestinal tract affect the severity of COVID-19 and for the first time provide indications that the virus might replicate in gut bacteria. In order to further support these findings, in the present work, cultures of bacteria from the human microbiome and SARS-CoV-2 were analyzed by electron and fluorescence microscopy. The images presented in this article, in association with the nitrogen (15N) isotope-labeled culture medium experiment, suggest that SARS-CoV-2 could also infect bacteria in the gut microbiota, indicating that SARS-CoV-2 could act as a bacteriophage. Our results add new knowledge to the understanding of the mechanisms of SARS-CoV-2 infection and fill gaps in the study of the interactions between SARS-CoV-2 and non-mammalian cells. These findings could be useful in suggesting specific new pharmacological solutions to support the vaccination campaign.
A Systematic Review and a Meta-Analysis of the Yellow Fever Vaccine in the Elderly Population
Authors: de Abreu Ariane de Jesus Lopes , Cavalcante João Roberto , de Araújo Lagos Letícia Wigg , Caetano Rosângela , Braga José Ueleres
Published: May 2022

We conducted a systematic review and a meta-analysis to assess the risk of serious adverse events in the elderly after yellow fever vaccination compared to the non-elderly population. We searched multiple databases and grey literature, and we selected research without language and publication date restrictions. Studies were analyzed in a descriptive way and meta-analyzed and expressed in terms of prevalence ratio and risk ratio with a 95% confidence interval, depending on the degree of heterogeneity found. A total of 18 studies were included and 11 were meta-analyzed. The results obtained through the meta-analysis showed a risk of serious adverse events after yellow fever vaccination three times higher for the elderly when compared to the non-elderly population and five times higher for persons > 70 years. In relation to adverse event types, viscerotropic disease associated with the yellow fever vaccine had a risk that was six times higher when compared to the population < 60 years. The evidence found supports that the vaccine indication in individuals > 60 years of age should be based on a careful analysis of individual benefit-risk assessments. The results found suggest a higher risk of events for individuals > 70 years, especially for viscerotropic and neurotropic disease associated with YFV contraindicating the use of the YFV in this age group.
New Onset of Eosinophilic Granulomatosis with Polyangiitis Following mRNA-Based COVID-19 Vaccine
Authors: Nappi Emanuele , De Santis Maria , Paoletti Giovanni , Pelaia Corrado , Terenghi Fabrizia , Pini Daniela , Ciccarelli Michele , Selmi Carlo Francesco , Puggioni Francesca , Canonica Giorgio Walter , Heffler Enrico
Published: May 2022

Anti-SARS-CoV-2 vaccines are safe and effective, also in individuals with allergic and immune-mediated diseases (IMDs). There are reports suggesting that vaccines may be able to trigger de-novo or exacerbate pre-existing IMDs in predisposed individuals. Eosinophilic granulomatosis with polyangiitis (EGPA) is a small-vessel vasculitis characterized by asthma, eosinophilia, and eosinophil-rich granulomatous inflammation in various tissues. We describe the case of a 63-year-old man who experienced cardiac, pulmonary, and neurological involvement one day after the administration of the booster dose of anti-SARS-CoV-2 vaccine (mRNA-1273). A diagnosis of EGPA was made and the patient was treated with high-dose steroids and cyclophosphamide, with a good clinical response. Interestingly, our patient had experienced a significant worsening of his pre-existing asthma six months earlier, just after the first two vaccine shots with the ChAdOx1 anti-SARS-CoV-2 vaccine. It is impossible to know whether our patient would have had developed EGPA following natural SARS-CoV-2 infection or at some point in his life regardless of infectious stimuli. Nevertheless, our report may suggest that caution should be paid during the administration of additional vaccine doses in individuals who experienced an increase in IMD severity that persisted over time following previous vaccine shots.
Copper Bioremediation Ability of Ciliate Paramecium multimicronucleatum Isolated from Industrial Wastewater
Authors: Liaqat Ayesha , Zahra Itrat , Abbas Syed Zaghum , Wabaidur Saikh Mohammad , Eldesoky Gaber E. , Islam Md Ataul , Rafatullah Mohd , Shakoori Farah R. , Shakoori Abdul R.
Published: May 2022

The growing problems of environmental damage have been caused by the continuous outrush of heavy metals from industrial wastewater. To resolve this issue, bioremediation is playing a safe and eco-friendly role in the removal of these heavy metals from environmental wastewater bodies. It has provoked demand with regard to understanding the mechanisms of bioaccumulation and detoxification developed by the organisms living in the heavy metal-exposed industrial wastewater. The present investigation focuses on Paramecium multimicronucleatum, a ciliated protozoan isolated from industrial wastewater, with the objective of assessing its capabilities as an environmental bioremediator. Purified cell culture was maintained in bold basal salt medium and optimum growth conditions were determined. A maximum growth rate of 6.0–9.0 × 103 cells/mL at 25–30 °C and pH 7.0 was observed, and therefore revealed to be the optimal growth conditions for this species. It can tolerate 40–50 µg/mL of copper ion stress with little effect on growth rate as compared to control. It is able to uptake more than 80% of copper ions from the medium in 96 h. A significant twofold rise in glutathione content and non-protein thiols was recorded as an indication of a defensive mechanism in place to fight against the oxidative stress caused by the copper treatment. A notable increase of 50–70 µg/mL in total protein content of stressed cells in comparison to non-stressed was also observed as potential induction of some particular proteins for the purpose of resistance against copper stress.
Changes in Organics and Nitrogen during Ozonation of Anaerobic Digester Effluent
Authors: Akter Jesmin , Lee Jaiyeop , Kim Weonjae , Kim Ilho
Published: May 2022

The objective of this study is to investigate the consequence of ozone dosage rate on the qualitative change in organic compounds and nitrogen in anaerobic digester effluent during the ozone process. Therefore, ozonation improves the biodegradability of recalcitrant organic compounds, quickly oxidizes the unsaturated bond, and forms radicals that continue to deteriorate other organic matter. In this study, ozonation was performed in a microbubble column reactor; the use of microbubble ozone improves the status of chemical oxygen demand (COD) and changes of organic nitrogen to inorganic compounds. The ozone injection rates were 1.0, 3.2, and 6.2 mg/L/min. The samples obtained during the ozone treatments were monitored for CODMn, CODCr, TOC, NO2−-N, NO3−-N, NH4+-N, T-N, and Org-N. The ozone dose increased 1.0 to 6.2 mg/L and it increased the degradation ratio 40% and the total organic carbon 20% during 20 min of reaction time. During the ozonation, the CODCr and CODMn values were increased per unit of ozone consumption. The ozone treatment showed organic nitrogen mineralization and degradation of organic compounds with the contribution of the microbubble ozone oxidation process and is a good option for removing non-biodegradable organic compounds. The original application of the microbubble ozone process, with the degradation of organic compounds from a domestic wastewater treatment plant, was investigated.
Physics-Guided Long Short-Term Memory Network for Streamflow and Flood Simulations in the Lancang–Mekong River Basin
Authors: Liu Binxiao , Tang Qiuhong , Zhao Gang , Gao Liang , Shen Chaopeng , Pan Baoxiang
Published: May 2022

A warming climate will intensify the water cycle, resulting in an exacerbation of water resources crises and flooding risks in the Lancang–Mekong River Basin (LMRB). The mitigation of these risks requires accurate streamflow and flood simulations. Process-based and data-driven hydrological models are the two major approaches for streamflow simulations, while a hybrid of these two methods promises advantageous prediction accuracy. In this study, we developed a hybrid physics-data (HPD) methodology for streamflow and flood prediction under the physics-guided neural network modeling framework. The HPD methodology leveraged simulation information from a process-based model (i.e., VIC-CaMa-Flood) along with the meteorological forcing information (precipitation, maximum temperature, minimum temperature, and wind speed) to simulate the daily streamflow series and flood events, using a long short-term memory (LSTM) neural network. This HPD methodology outperformed the pure process-based VIC-CaMa-Flood model or the pure observational data driven LSTM model by a large margin, suggesting the usefulness of introducing physical regularization in data-driven modeling, and the necessity of observation-informed bias correction for process-based models. We further developed a gradient boosting tree method to measure the information contribution from the process-based model simulation and the meteorological forcing data in our HPD methodology. The results show that the process-based model simulation contributes about 30% to the HPD outcome, outweighing the information contribution from each of the meteorological forcing variables (<20%). Our HPD methodology inherited the physical mechanisms of the process-based model, and the high predictability capability of the LSTM model, offering a novel way for making use of incomplete physical understanding, and insufficient data, to enhance streamflow and flood predictions.
Graphene Oxide-Chitosan Composites for Water Treatment from Copper Cations
Authors: Politaeva Natalia , Yakovlev Andrei , Yakovleva Elena , Chelysheva Valentina , Tarantseva Klara , Efremova Sania , Mukhametova Liliya , Ilyashenko Svetlana
Published: May 2022

This paper considers modern sorption materials for wastewater treatment. The literature data on wastewater treatment with materials based on graphene and chitosan are presented. The production and application of composite sorbents is discussed. It is shown that a promising application of graphene oxide (GO) as a filler enhances the mechanical and sorption properties of the polymer matrix. The biopolymer chitosan (Ch) is a challenging matrix for GO, having unique sorption, chelate-forming, ion-exchange, and complex-forming properties. Composite adsorbents based on graphene oxide and chitosan have a high extraction efficiency of heavy and radioactive metals, dyes, and pharmaceutical compounds dorzolamide and tetracycline. GO-Ch composites with various ratios of chitosan and graphene oxide (2–7%) were formed by drop granulation. The composites obtained were investigated in terms of the ability to extract copper cations from the effluents, and it was shown that the composite having the content of GO:Ch = 55.5:44.5% (by mass in dry granules) had the best sorption and mechanical properties. This sample had high purification efficiency from copper cations (96%) and the required mechanical properties (attrition ≤ 0.4%, grindability ≤ 4%). For this sample, the influence of various factors (pH, sorbent dosage, temperature, and time of sorption) on sorption processes were studied. The best conditions for the sorption processes by the GO-Ch sorbent were determined. The sorbent dosage was 20 g/L, the sorption time was 20 min, and the temperature was 20 ± 2 °C, pH = 7. The adsorption isotherm was plotted and the maximum sorption capacity of copper cations A = 58.5 mg/g was determined. Microstructural and infrared (IR) spectroscopy studies of GO-Ch composites showed the presence of a porous surface and OH- and C=O functional groups. A mechanism for the extraction of copper cations due to physical sorption of the porous surface by GO-Ch composites, and due to chemisorption processes by functional groups, was proposed. The sorption properties for methylene blue and iodine absorption, and the specific surface area of the GO-Ch samples, were determined. The spent sorbent is proposed to be used as a soil improver.
Monitoring and Mapping of Soil Salinity on the Exposed Seabed of the Aral Sea, Central Asia
Authors: Duan Zihao , Wang Xiaolei , Sun Lin
Published: May 2022

The incredible drying of the Aral Sea has resulted in a large area of exposed seafloor with saline soils, which has led to catastrophic consequences. This study investigated ground-truth soil salinity data and used Landsat data to map the soil salinity distribution of the exposed seabed of the Aral Sea from 1960 onwards. The soil salinity distribution, with the depth from 0 cm to 100 cm, was analyzed. The correlation analysis was applied to find the best performance index in describing soil salinity changes. The results showed that ground-truth data of topsoil salinity (depth of 0−5 cm) exhibited a significantly strong correlation with soil salinity index 4 (SI4) among seven indices, where the Pearson correlation coefficient (r) was up to 0.92. Based on the relationship between soil salinity sampling data and SI4, a linear regression model was employed to determine the capability of evaluating the soil salinity distribution of the Aral Sea with the coefficient of determination (R2), root mean squared error (RMSE), and ratio of performance to deviation (RPD) values of 0.84 and 0.86 dS m−1 and 2.36, respectively. The SI4 performed well and was used to predict the soil salinity distribution on the exposed seabed. The distribution showed that soil salinity increased from the former to current shoreline. In the North Aral Sea, compared to 1986, the water area remained stable, accounting for 50.3% in 2020, and the soil salinization level was relatively low. However, the moderately and slightly saline areas dominated 73.8% and 7.5% of the South Aral Sea in 2020, with an increase of 53% and 6% transformed from the water area. The area of salinized soils dramatically increased. The strongly and extremely saline areas were mainly located in the northeastern part of the eastern basin and western part of Vozrozhdeniya Island, respectively, and were the main source of salt-dust storms. These results support the dynamic monitoring and distribution patterns of soil salinization in the Aral Sea.
Biodiversity of Non-Marine Ostracoda (Crustacea) of Botswana: An Annotated Checklist with Notes on Distribution
Authors: Szwarc Agata , Namiotko Tadeusz
Published: May 2022

Botswana constitutes a major gap in our knowledge of the distribution of Ostracoda in the region of Southern Africa, restraining thorough biogeographic interpretations. We combine records from previously published surveys along with our own field collections to provide a collation of living and fossil (Late Pleistocene to Holocene) Ostracoda recorded in Botswana. Our survey yielded 17 species, of which nine species have not been recorded before in the country. Including the present update, 54 species (45 living and nine fossil or subfossil) belonging to 22 genera of five families (with 76% species belonging to the family Cyprididae) are currently reported from Botswana. Yet, 23 taxa are left in open nomenclature, indicating the urgent need for sound systematic studies on harmonizing taxonomy of Southern African ostracods, especially of those inhabiting small temporary waterbodies, considered as threatened with extinction before being properly described or discovered. This updated checklist provides detailed information about the distribution and habitat of each recorded species. Species richness, distribution patterns, and diversity of ostracod species regionally and in different freshwater ecoregions are also discussed. We found low alpha (site) diversity (mean 3.3 species per site) and a significant difference in species composition and beta diversity of the Okavango ecoregion versus the Kalahari and Zambezian Lowveld ecoregions.
Large Scale Laboratory Experiment: The Impact of the Hydraulic Characteristics of Flood Waves Caused by Gradual Levee Failure on Inundation Areas
Authors: Yoon Kwang Seok , Rehman Khawar , Yoo Hyung Ju , Lee Seung Oh , Hong Seung Ho
Published: May 2022

As a levee failure and the consequent flooding cause significant financial losses and sometimes human casualties, they have led to considerable concern among city officials. Therefore, researchers have devoted considerable effort to investigating the hydraulic characteristics of sudden transient flow in the form of propagated waves to inundation areas during a levee and/or dam failure. A large number of studies, however, have mostly focused on simple one-dimensional cases investigated numerically and/or experimentally, and thus, important hydraulic characteristics, particularly near the failure zone, have not been adequately captured because of three-dimensional complexities. Taking these complexities into consideration, this study conducts a large-scale experiment to examine the characteristics of wave propagation in an open area caused by a gradual levee failure. From the experimental observations, this study provides the propagation speed of a wave front and suggests a formula for the maximum flood depth corresponding to the peak flood wave in the inundation area. We expect the findings to provide hydraulic engineers and scientists with fundamental insights into transient flow during a gradual levee failure. By contributing to our theoretical understanding, the measurements can also be used as validation tools for future numerical simulation and are likely to contribute to the establishment of emergency action plans that can help city officials cope with flood inundation.
Detection and Quantification of Dam Leakages Based on Tracer Tests: A Field Case Study
Authors: Qiu Huiyang , Hu Rui , Huang Yong , Gwenzi Willis
Published: May 2022

Leakage is a common phenomenon in dams, and its early detection is critical for dam safety. In the present study, a new method based on tracer tests is applied to detect and quantify leakage in the Wanyao Dam, Jiangshan City, China. The objective is to detect the leakage zone of a dam wall by combining the natural tracer test and the artificial tracer test. Temperature, electrical well-logging tests with nature tracers, and the artificial tracer test with salt (NaCl) were conducted using 48 and 5 pre-existing boreholes, respectively. Using natural tracer tests, the 48 boreholes are categorized into 4 leakage classes: (1) Class 1, high connectivity within whole borehole; (2) Class 2 high connectivity at lower depths; (3) Class 3, weaken connectivity; and (4) Class 4, safe boreholes with no connectivity. Using the proposed method, specific leakage rates of some boreholes were estimated. The results of the new method are validated by comparison with those from natural tracer tests, site-investigation, and historical observation data. Overall, the new tracer test has the following merits: (1) low cost, (2) environment friendliness, and (3) is simple to apply. Moreover, the proposed method improves the accuracy of traditional tracer tests for detecting leakage zones.
Improving the Modified Universal Soil Loss Equation by Physical Interpretation of Its Factors
Authors: Tsige Manaye Getu , Malcherek Andreas , Seleshi Yilma
Published: May 2022

A primary objective of this paper is to change the input data requirement of the Modified Universal Soil Loss Equation (MUSLE) for the calculation of its runoff factor for possible application in data-scarce areas. Basically, the MUSLE was developed for a small agricultural watershed, where the extent of erosion is from sheet to rill erosion, but we cannot exactly tell whether it considers gully erosion or not. The underlying physical assumption to improve the MUSLE is that the amount of potential energy of runoff is proportional to the shear stress for sediment transport from a slope field and the kinetic energy of the runoff at the bottom of the slope field for gully formation. The improved MUSLE was tested at four watersheds in Ethiopia, and it showed better performance (i.e., the minimum performance is 84%) over the original MUSLE (i.e., the minimum performance was 80%), for all four watersheds under our consideration. We expect the same to be true for other watersheds of Ethiopia.
Large-Scale Surface Water Mapping Based on Landsat and Sentinel-1 Images
Authors: Tang Hailong , Lu Shanlong , Ali Baig Muhammad Hasan , Li Mingyang , Fang Chun , Wang Yong
Published: May 2022

Surface water is a highly dynamical object on the earth’s surface. At present, satellite remote sensing is the most effective way to accurately depict the temporal and spatial variation characteristics of surface water on a large scale. In this study, a region-adaptive random forest algorithm is designed on the Google Earth Engine (GEE) for automatic surface water mapping by using data from multi-sensors such as Landsat 7 ETM+, Landsat 8 OLI, and Sentinel-1 SAR images as source data, and China as a case study region. The visual comparison of the mapping results with the original images under different landform areas shows that the extracted water body boundary is consistent with the water range in the image. The cross-validation with the JRC GSW validation samples shows a very high precision that the average producer’s accuracy and average user’s accuracy of water is 0.933 and 0.998, respectively. The average overall accuracy and average kappa is 0.966 and 0.931, respectively. The independent verification results of lakes with different areas also prove the high accuracy for our method, with a maximum average error of 3.299%. These results show that the method is an ideal way for large-scale surface water mapping with a high spatial–temporal resolution.
The Dutch Flood Protection Programme: Taking Innovations to the Next Level
Authors: Tromp Ellen , te Nijenhuis Anouk , Knoeff Han
Published: May 2022

The Dutch regional water authorities face an enormous task: the strengthening of about 1500 km of dikes and 500 civil-engineering structures before 2050. This immense operation is being funded, prioritised and supported by the Dutch Flood Protection Programme (DFPP), an alliance of regional water authorities and the Ministry of Infrastructure and Water Management. The work will be executed in nearly 300 projects located throughout the country on the coast, lakes and major rivers. To complete this task on time and within budget, innovation (a better insight into the behaviour of flood defences, new techniques and processes) is believed to be the way forward. In this paper, we look at how the DFPP has encouraged innovations between 2012 and the present. We stress the importance of using a sender–receiver approach to further knowledge transfer and uptake, and we describe how, by using an action research approach, the Dutch Flood Protection Programme is currently adapting its innovation strategy on the basis of lessons learned to improve knowledge uptake. We will address some of the innovations that have been developed over the years and how monitoring knowledge uptake helps to further improve the learning-by-doing approach.