bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated Analysis of Osmotic Stress and Infrared Thermal Imaging for the Selection of Resilient Rice Under Water Scarcity
Authors: Mahreen Naima , Yasmin Sumera , Asif M. , Yousaf Sumaira , Yahya Mahreen , Ejaz Khansa , Shahid Hussain Hafiz , Sajjid Zahid Iqbal , Arif Muhammad
Published: February 2022

The climate change scenario has increased the severity and frequency of drought stress, which limits the growth and yield of rice worldwide. There is a dire need to select drought-tolerant rice varieties to sustain crop production under water scarcity. Therefore, the present study effectively combined morpho-physiological and biochemical approaches with the technology of infrared thermal imaging (IRTI) for a reliable selection of drought-tolerant genotypes. Initially, we studied 28 rice genotypes including 26 advance lines and three varieties for water stress tolerance under net house conditions. Three genotypes NIBGE-DT-02, KSK-133, and NIBGE-DT-11 were selected based on the Standard Evaluation System (SES) scoring for drought tolerance. NIBGE-DT-02 showed tolerance to polyethylene glycol (20%) induced osmotic stress indicated by a minimum reduction in seedling length, biomass, chlorophyll content, and increased leaf proline content as compared to susceptible varieties under a hydroponic system. NIBGE-DT-02 was further evaluated for water withholding at varying growth stages, i.e., 30 and 60 days after transplantation (DAT) in pots under net house conditions. NIBGE-DT-02 showed a significantly lower reduction (35.9%) in yield as compared to a susceptible variety (78.06%) under water stress at 60 DAT with concomitant induction of antioxidant enzymes such as peroxidase, catalase, and polyphenol oxidase. A significant increase (45.9%) in proline content, a low increase (7.5%) in plant temperature, along with a low reduction in relative water content (RWC) (5.5%), and membrane stability index (MSI) (9%) were observed under water stress at 60 DAT as compared to the well-watered control. Pearson correlation analysis showed the strong correlation of shoot length with MSI and root length with RWC in rice genotypes at the later growth stage. Furthermore, Regression analysis indicated a negative correlation between plant temperature of NIBGE-DT-02 and proline, RWC, MSI, and peroxidase enzyme under variable water stress conditions. All these responses collectively validated the adaptive response of selected genotypes under water stress during different growth stages. Tolerant genotypes can be used in breeding programs aimed at improving drought tolerance and can expand rice cultivation. Furthermore, this study provides a foundation for future research directed to utilize IRTI as a fast and non-destructive approach for the selection of potent rice genotypes better adapted to water scarcity from wide germplasm collection.
Trading Patients: Applying the Alternative Model for Personality Disorders to Two Cases of DSM-5 Borderline Personality Disorder Over Time and Across Therapists
Authors: Bliton Chloe F. , Rosenstein Lia K. , Pincus Aaron L.
Published: February 2022

TheDSM-5Alternative Model for Personality Disorders (AMPD) dimensionally defines personality pathology using severity of dysfunction and maladaptive style. As the empirical literature on the clinical utility of the AMPD grows, there is a need to examine changes in diagnostic profiles and personality expression in treatment over time. Assessing these changes in individuals diagnosed with borderline personality disorder (BPD) is complicated by the tendency for patients to cycle through multiple therapists over the course of treatment leaving the potential for muddled diagnostic clarity and disjointed case conceptualizations. Following patient trajectories across therapists offers a unique opportunity to examine the AMPD’s sensitivity to and utility for capturing personality stability and change over time for patients with BPD. This article demonstrates the utility of the AMPD for two clinical cases in three distinct ways: (i) highlighting heterogeneity in BPD between patients, (ii) comparing improvements in personality severity and style over time, and (iii) elucidating profile change across therapist ratings. We present two patients diagnosed withDSM-5 Section II BPD, crossing between two therapists over the course of 3 years of psychodynamic psychotherapy. Treating clinicians rated patients for their respective treatment phases using the Level of Personality Functioning Scale (LPFS), capturing severity, and the Personality Inventory for theDSM-5(PID-5), capturing style. AMPD diagnostic profiles differentiated patients with BPD in both severity and style, and captured within-patient change beyond within-therapist response bias. Results indicated greater improvements in personality severity while personality style remained more stable. Implications for the patients’ treatment progress and associated challenges are discussed, as are considerations for the utility of the AMPD in therapy.
Monte Carlo Simulation of Clothed Skin Exposure to Electromagnetic Field With Oblique Incidence Angles at 60 GHz
Authors: Li Kun , Sasaki Kensuke
Published: February 2022

This study presents an investigation of clothed human skin exposure to obliquely incident electromagnetic waves at 60 GHz. We clarified the combined impacts of the cloth material, incidence angle, and polarization on the assessment of transmittance and absorbed power density (APD) at the skin surface. A Monte Carlo simulation was conducted considering the thickness variation of the cloth material and skin tissue. For the case of transverse magnetic™ wave exposure, the transmittance increases with increasing incident angle up to the maximum transmittance angle in the range from 60 to 80°, which is known as the Brewster effects, regardless of textile materials and air gap between cloth and skin. The air gap results in a periodic fluctuation of the APD, where the variation is almost within 1 dB when the incident power density is constant and the incident angle is smaller than 40°. Our results also show that as the air gap increases to 2.5 mm, i.e., half-wavelength at 60 GHz in the air, the APD within the skin surface covered by typical cloth materials increases up to 40% compared with that of bare skin. Although the use of several cloth materials may increase the transmittance and APD in oblique incidence scenarios, all the results of the APD do not exceed the basic restriction for local exposure, demonstrating that the current guidelines for human exposure to electromagnetic fields are appropriate for preventing the excessive exposure at 60 GHz considering the impacts of oblique incidence angles and cloth materials.
Diffuse Cerebral Vasospasm After Aneurysmal Subarachnoid Hemorrhage in a 15-Year-Old Girl: A Case Report
Authors: Lee Dajeong , Lee Jeehun , Kim Keon Ha , Kim Ji Hye , Lee Jiwon
Published: February 2022

Diffuse cerebral vasospasm after subarachnoid hemorrhage (SAH) is a complication resulting in an ischemic condition presenting with altered mentality and followed by motor or speech impairment. It is uncommon in pediatric population and requires differential diagnosis from Moyamoya disease, which is relatively common in Korea. We report a case of a 15-year-old girl who was presented with a seizure and subsequent headache, poor oral intake, and altered mentality, who was finally diagnosed with sporadic vasospasm followed by multiple aneurysm ruptures. The patient had recurrent seizures and persistent headache at the time of transfer. On the second day after transfer, she showed focal motor weakness and dysarthria, and her symptoms gradually progressed, showing paraplegia and aphasia on the third hospitalization day. Brain magnetic resonance imaging and magnetic resonance angiography demonstrated diffuse narrowing cerebral vasospasm of bilateral middle cerebral arteries, anterior cerebral arteries, and distal internal carotid arteries and three unruptured aneurysms. The patient was treated with intravenous hydration and nimodipine to expand the narrowed vessels. After confirming that the vessels were enlarged, we successfully executed the endovascular coil embolization. Her neurological deficits were improved through medical, interventional, and rehabilitation treatments and fully restored 11 months after discharge.
Virtual Reality for Interactive Medical Analysis
Authors: Koger Casey R. , Hassan Sohail S. , Yuan Jie , Ding Yichen
Published: February 2022

Molecular imaging along with 3-dimensional (3-D) or 4-D (3-D spatial + 1-D temporal) visualization is widely used in clinical diagnosis and surgical planning. However, the pre-defined perspective and confined manipulation limit the in-depth exploration and analysis in 3-D/4-D. To overcome this obstacle, we utilized virtual reality (VR) to interact with CT images of the cardiopulmonary system in a 3-D immersive environment. We implemented manipulative functionalities into the VR environment that altered the cardiopulmonary models to interactively generate new data analysis perspectives. We successfully sliced a CT cardiac model showing in-depth surface visualizations of the ventricles and atria. Our customized framework enables enhanced data interpretation interactivity of CT images and establishes a user-directed manipulative VR platform derived from imaging results for remote medical practices including training, education, and investigation.
Preliminary Study on Immediate Postoperative CT Images and Values of the Modular Polyetheretherketone Based Total Knee Arthroplasty: An Observational First-in-Human Trial
Authors: Cai Zhengyu , Qu Xinhua , Zhao Yaochao , Yuan Zhiguo , Zheng Liangjun , Long Teng , Yao Qiuying , Yue Bing , Wang You
Published: February 2022

Background

Total knee arthroplasty (TKA) is now frequently performed and is highly successful. However, patient satisfaction after TKA is often difficult to achieve. Because of the presence of metallic prosthetic knee joints, there is a lack of imaging tools that can accurately assess the patient's postoperative prosthetic position, soft tissue impingement, and periprosthetic bone density after TKA. We conducted a clinical trial of the world's first totally modular polyetheretherketone (PEEK) TKA and determined the bone density values in the stress concentration area around the prosthesis based on postoperative computed tomography data to reconstruct a three-dimensional model of the PEEK prosthetic knee joint after implantation. Based on the model, the overhang of the prosthesis was measured at various locations on the prosthesis.

Methods

All patients who underwent PEEK-based TKA were postoperatively assessed with radiography and computed tomography (CT). Hounsfield units (HUs) for the different components of the quantitative CT assessment were measured separately.

Results

Ten patients (nine female and one male) aged 59–74 (mean 66.9, median 67) years were included. The HU values were as follows: PEEK prosthesis mean 182.95, standard deviation (SD) 4.90, coefficient of variation (CV) 2.68; polyethylene mean −89.41, SD 4.14, CV −4.63; lateral femoral osteochondral mean 192.19, SD 55.05, CV 28.64; lateral tibial osteochondral mean 122.94, SD 62.14, CV 42.86; medial femoral osteophyte mean 180.76, SD 43.48, CV 24.05; and medial tibial osteophyte mean 282.59, SD 69.28, CV 24.52. Analysis of the data at 1, 3, and 6 months showed that the mean PE (p = 0.598) and PEEK (p = 0.916) measurements did not change with the time of measurement. There was a decrease in bone mineral density in the lateral tibia at 3 months (p = 0.044). Otherwise, there was no significant change in bone density in other regions (p = 0.124–0.803). There was no overhang in all femoral prostheses, whereas there were two cases of overhang in tibial prostheses. Overhang measurements do not differ significantly across time points. The overhang measurements were not significantly different at all time points (p = 0.186–0.967).

Conclusion

PEEK knee joint prosthesis has excellent CT compatibility. The change in periprosthetic bone volume during the follow-up period can be determined using the HU value after CT scan, while the prosthesis position can be assessed. This assessment may potentially guide future improvements in knee prosthesis alignment techniques and artificial knee prosthesis designs.
A Preliminary Cadaveric MRI Study of Fetal Hip Development
Authors: Liu Zhenqing , Li Huixian , Wang Shuai , Wu Qianqian , Liu Hongsheng
Published: February 2022

Purpose

The earlier the detection of the hip joint is discovered, the better the final result. The purpose of this study aimed to investigate the fetal hip development using magnetic resonance imaging (MRI), so as to alert clinicians to possible abnormal development during intrauterine life.

Method

Measurements of 34 cadaver fetuses (68 hips) were obtained regarding acetabular width and depth, anterior bony acetabular index (ABAI), anterior cartilaginous acetabular index (ACAI), posterior bony acetabular index (PBAI), and posterior cartilaginous acetabular index (PCAI). The standard values of each acetabular measurement index were obtained, and the gestational age-measurement index change trend chart was drawn to comprehensively analyze the normal development law of the fetal hip joint.

Results

With the development of fetuses, the width and depth of acetabular increase linearly, and the slope of acetabular width was larger than that of depth. In addition, two change points during the 24th and 34th weeks of gestation were detected with regard to width. ABAI and PBAI also decreased. ABAI demonstrated an approximately linear trend, while PBAI shows a non-linear trend. During the 36th week, the change point in PBAI was observed. ACAI and PCAI exhibited slow increases, indicating a non-linear trend. During the 21st and 36th weeks of gestation, the change points regarding ACAI were observed. During the 22nd week of gestation, the change point for PCAI was observed.

Conclusion

Plots of the parameters obtained via MRI examinations of cadaver fetuses across gestational age comprehensively illustrated the fetal hip development. This developmental information about the hip joint has the potential to guide clinicians in the early detection of abnormal hip joint development during intrauterine life.
Smallholder Broiler Farmers' Characteristics to Uptake Measures Against Highly Pathogenic Avian Influenza in Western Java
Authors: Pramuwidyatama M. Gumilang , Indrawan Dikky , Saatkamp Helmut W. , Hogeveen Henk
Published: February 2022

Highly Pathogenic Avian Influenza (HPAI) H5N1 remains endemic in the Western Java smallholder broiler farms. This study aims to identify farmers and farm characteristics associated with farmers' motivations toward five different measures directed to HPAI: cleaning and disinfection (C&D), vaccination, reporting, and stamping-out with and without compensation. Through multi-stage sampling and a questionnaire, we collected data from 199 farmers in Western Java and applied descriptive analysis and logistic regression to evaluate the data. Most smallholder broiler farms had a production contract with a poultry company. Unexpectedly, we identified subtypes of price-contract (i.e., revenues based on contract selling price and live bird weight) and makloon-contract (i.e., revenues based on management fee per bird) schemes. We identified these new subtypes as extended price-contract and extended makloon-contract schemes. These extended subtypes included issues related to animal health management and payment schemes. The results show that most of the farmers in both extended types were highly motivated to implement C&D and vaccination. Business types and farmers' awareness of HPAI were significantly associated with a farmer's motivation to implement C&D. Farmers who had an awareness of HPAI were more likely to implement C&D. Although our models were insufficient to model the association of farmers' motivation to uptake preventive measures against HPAI in Western Java, this study identified significant characteristics that help improve HPAI control policy in Western Java. Our study suggests that farm business types and incentives through payment schemes and training may increase the uptake of preventive measures by farmers.
Expression of Carboxypeptidase A3 and Tryptase as Markers for Lymph Node Metastasis of Canine Cutaneous Mast Cell Tumors
Authors: Thaiwong Tuddow , Cirillo Juliana V. , Heller Jane , Kiupel Matti
Published: February 2022

Detection of metastatic mast cell tumors (MCTs) in lymph nodes is a critical factor for treatment, prognosis, and clinical management. Presence/absence of mast cells in the lymph nodes cannot be used as a sole parameter to determine metastasis due to the inability to differentiate neoplastic from non-neoplastic/inflammatory mast cells. While cytologic and histopathologic classifications for assessment of metastatic MCTs based on the numbers and distribution of mast cells have been developed, inconsistency between the clinical interpretation of these grading schemes and actual metastatic status occurs. The aim of this study is to identify a novel diagnostic tool to accurately predict overt metastatic mast cell tumors in lymph nodes. We investigated the possibility of using RT-qPCR to detect mRNA expression of mast cell-specific genes in lymph nodes with different stages of MCT metastatic classification. We are able to establish a highly sensitive and discriminating RT-qPCR measuring Carboxy peptidase A3 (CPA3) and tryptase mRNA expression and identify the cut-off values with high sensitivity and specificity for overt metastatic MCTs in lymph nodes. An area of future interest would be to expand our analysis of the extent to which cut-off values for these markers in correctly identifying disease status, as well as predicting clinical outcomes and survival times. This would offer valuable information regarding the practical applicability of this technique and may enable us to improve our standards of detection metastasis, including possibility of molecular analysis of cytologic specimens obtained from suspicious nodes subjected to surgical excision.
Fucosyltransferase 8 is Overexpressed and Influences Clinical Outcomes in Lung Adenocarcinoma Patients
Authors: Liang Yiqian , Wang Ting , Gao Rui , Jia Xi , Ji Ting , Shi Puyu , Xue Jianjun , Yang Aimin , Chen Mingwei , Han Peng
Published: February 2022

Background: Lung adenocarcinoma (LUAD), the most prevalent type of lung cancer, is often metastatic and has a poor prognosis. Recent studies have demonstrated an important role for fucosyltransferase 8 (FUT8) in carcinogenesis and cancer progression.

Methods: A meta-analysis with 15 eligible datasets from Gene Expression Omnibus (GEO) was performed to explore the expression of FUT8 in LUAD. The results were further verified in The Cancer Genome Atlas (TCGA) database, followed by survival analysis using Kaplan-Meier plotter. We also validated the protein expression of FUT8 by immunohistochemistry (IHC). In vitro experiments were conducted to determine the biological effects of FUT8 in LUAD cells.

Results: The meta-analysis showed the FUT8 expression in LUAD tissues was significantly higher than those in normal lung tissues [standard mean difference (SMD): 1.40; 95% confidence interval (CI): .95–1.85]. The results of TCGA database verified the expression of FUT8 increased in LUAD tissues versus normal tissues. IHC analyses indicated that the protein levels of FUT8 were up-regulated in LUAD, and elevated FUT8 expression was significantly correlated with poor prognosis in LUAD patients. Multivariable Cox regression analysis revealed that FUT8 expression was an independent prognostic factor. Besides, in vitro experiments showed that knockdown of FUT8 in LUAD cells markedly restrained cell proliferation, and stimulated cell apoptosis.

Conclusion: This study indicates that increased FUT8 expression is correlated with shortened survival of LUAD patients and might favor the progression of the disease.
Design of a Low-Cost Air Bearing Testbed for Nano CMG Maneuvers
Authors: Papakonstantinou Charalampos , Moraitis Georgios , Lappas Vaios , Kostopoulos Vassilis
Published: February 2022

In this paper, a low-cost, miniature spacecraft attitude control simulator is presented for testing miniature actuators such as Nano Control Moment Gyroscopes (CMGs) for simple maneuvers. The experimental setup is composed by an attitude control system (ACS) that mainly consists of a four-CMG cluster in a pyramid configuration and a custom-made air bearing. The one-degree-of-freedom (DoF) air bearing is fabricated to reproduce the frictionless conditions of a nano-satellite in orbit. The ACS is made exclusively using low-cost commercial-off-the-shelf (COTS) components, whilst the air bearing is made using 3D-printed parts. Both hardware and software implementations are described in detail and the performance of the developed simulator is evaluated by two maneuver experiments. Despite the manufacturing imperfections, the ACS is capable of providing higher angular velocities than previously presented in the literature while following the theoretical or simulation data. The results indicate that it is possible to manufacture a low-cost, miniature actuator such as a CMG, using COTS components to demonstrate the operation of an agile nano-satellite. Any deviations from the theoretical values are addressed and several improvements are discussed to further enhance the performance of the air bearing testing platform.
Thermal Vacuum Test Campaign of the EIRSAT-1 Engineering Qualification Model
Authors: Dunwoody Rachel , Reilly Jack , Murphy David , Doyle Maeve , Thompson Joseph , Finneran Gabriel , Salmon Lána , O’Toole Conor , Reddy Akarapu Sai Krishna , Erkal Jessica , Mangan Joseph , Marshall Fergal , Somers Eoghan , Walsh Sarah , de Faoite Daithí , Hibbett Mike , Palma David , Franchi Loris , Ha Lily , Hanlon Lorraine , McKeown David , O’Connor William , Uliyanov Alexey , Wall Ronan , Shortt Brian , McBreen Sheila
Published: February 2022

CubeSats facilitate rapid development and deployment of missions for educational, technology demonstration, and scientific purposes. However, they are subject to a high failure rate, with a leading cause being the lack of system-level verification. The Educational Irish Research Satellite (EIRSAT-1) is a CubeSat mission under development in the European Space Agency’s (ESA) Fly Your Satellite! Programme. EIRSAT-1 is a 2U CubeSat with three novel payloads and a bespoke antenna deployment module, which all contribute to the complexity of the project. To increase the likelihood of mission success, a prototype model philosophy is being employed, where both an engineering qualification model (EQM) and a flight model of EIRSAT-1 are being built. Following the assembly of the EQM, the spacecraft underwent a successful full functional test and month-long mission test. An environmental test campaign in ESA Education Office’s CubeSat Support Facility was then conducted with the EQM where both vibration and thermal verification test campaigns were performed. The focus of this paper is the thermal testing and verification of the EIRSAT-1 EQM. Over three weeks, the EQM was subjected to one non-operational cycle, three and a half operational cycles, and a thermal balance test in a thermal vacuum chamber. After dwelling at each temperature extreme, functional tests were performed to investigate the performance of the spacecraft in this space representative environment. The approach to planning and executing the thermal testing is described in detail including the documentation required, set up of the test equipment, and determination of the test levels. Overall, the campaign demonstrated that the mission can successfully operate in a space environment similar to that expected in orbit, despite encountering a number of issues. These issues included a payload displaying anomalous behaviour at cold temperatures and needing to redefine test levels due to an insufficient understanding of the internal dissipation in the spacecraft. A total of two major and three minor non-conformances were raised. Crucially, these issues could not have been found without thermal testing, despite the comprehensive ambient tests performed. The main results and lessons learned during this thermal test campaign are presented with the aim of guiding future missions on optimal approaches in organising and executing the thermal testing of their CubeSats.
Intercropping Tuber Crops with Teak in Gunungkidul Regency, Yogyakarta, Indonesia
Authors: Maharani Dewi , Sudomo Aris , Swestiani Dila , Murniati  , Sabastian Gerhard E. , Roshetko James M. , Fambayun Rizki Ary
Published: February 2022

The adoption of agroforestry system aims to combine food production enhancement to compensate population growth with the improvement of agricultural marketable products to increase household income. The diversification of food crop products requires more effective land use. In Gunungkidul, high-density teak (Tectona grandis) plantation has dominated many private forests. The area under the tree crown has received low light intensity, where only shade-resistant plants can survive. Tuber crops, i.e., arrowroot (Maranta arundinacea), canna (Canna edulis) and yam (Dioscorea esculenta) are shade-tolerant crops, which were planted in tree understory for supplementary food production and income generation. The cultivation under teak stand has been overlooked due to uncertainty in tuber productivity. To address this knowledge gap, the effect of teak shade (5- and 7-year teak) on the growth and yield of the three tuber crops was examined. The results indicated that both teak trial areas (with RLI 45.13% and 38.76%) were suitable for canna production (LER > 1), while management options were recommended for enhancing arrowroot and yam production. The LER of intercropped three-tuber crops under 5 years’ teak were >1, while of those under 7 years’ teak, only canna reached >1. Canna is the preferred option to be mixed in teak agroforestry systems with low light intensity due to its consistent yields, whether planted in open area or under teak shade. Silvicultural management, pruning and thinning are recommended to increase the growing space and resource sharing for intercropped plants. Land optimization in private forest understory using shade-resistant tubers will offer medium-term benefits, provided that proper silvicultural procedures are applied.
The Use of Ecological Hydromulching Improves Growth in Escarole (Cichorium endivia L.) Plants Subjected to Drought Stress by Fine-Tuning Cytokinins and Abscisic Acid Balance
Authors: Romero-Muñoz Miriam , Albacete Alfonso , Gálvez Amparo , Piñero María Carmen , Amor Francisco M. del , López-Marín Josefa
Published: February 2022

Drought is considered as one of the major limiting factors to plant growth and productivity. Drought stress reduces stomatal conductance, affecting water relations and decreasing CO2 assimilation rate and photosynthesis. Several strategies have been developed to alleviate the negative effects of drought in the agricultural industry. One of these strategies is the use of the mulching technology, which retains water in the soil surface. Knowing that hormones play a key role in plant growth and drought stress responses, we hypothesized that the use of a new ecological mulching technology called hydromulching would improve growth over bare soil under drought stress through changes in the hormonal balance. To test this hypothesis, escarole plants (Cichorium endivia L.) were grown in pots filled with coco fiber, non-covered (bare soil) or covered with polyethylene film (PE) and three types of hydromulches made up with recycled additives: wheat straw (WS), rice hulls (RH), and substrate used for mushroom cultivation (MS). Half of the plants were subjected to drought by reducing the volume of irrigation water to 70% of crop evapotranspiration. Despite drought stress impaired escarole growth-related parameters in all treatments, plants mulched with MS maintained significantly superior growth, due to improved plant water relations and photosynthetic function. This can be explained by an efficient interaction hydromulch/soil/plant in regulating the hormonal balance under water depletion. Indeed, the concentrations of the active cytokinins (CKs), trans-zeatin and isopentenyladenine, were higher in plants grown with MS treatment, associated with shoot growth-enhancing and photosynthetic rate maintenance under stress conditions. The concentrations of the stress-related hormone, abscisic acid (ABA), varied antagonistically to those of the active CKs. In this regard, ABA increased with drought but to a lower extent in MS plants thus regulating stomata opening, which, in crosstalk with the ethylene precursor 1-aminocyclopropane-1-carboxylic acid and salicylic acid, improved plant water relations. The results obtained demonstrate that hydromulching is an efficient and sustainable management strategy to ameliorate the drought effects on escarole plants through fine regulation of the CKs/ABA balance, which will be of utmost interest and applicability in the actual climate change scenario.
Investigation of a Multivariate Correction Method for HVI Fibrogram Measurements
Authors: Sayeed Md Abu , Kelly Brendan R , Turner Christopher , Hequet Eric F
Published: February 2022

Cotton fiber length parameters are used across the cotton industry to select elite germplasm, purchase cotton bales and manage mill throughput. The High Volume Instrument (HVI) provides the most commonly used fiber length parameters, upper half mean length (UHML) and uniformity index (UI). UI is the ratio of mean length (ML) to the UHML expressed as a percentage. These length parameters, UHML and ML, are generated following the fibrograph principle and are highly correlated with two span lengths on the fibrogram curve. These two length parameters represent a small part of the fibrogram and do not characterize the total within-sample variation. In previous studies, we found that fiber length variation captured by the whole fibrogram improves the prediction of yarn quality. However, HVIs are currently calibrated for UHML and UI. In this study, we investigated a correction method using a set of 461 commercial samples to correct the whole fibrogram curve across HVIs and validated the method using an independent set of 932 commercial samples. The correction procedure lowers the Euclidian distance between fibrograms as much as 35%, bringing the fibrogram measurements into agreement across multiple HVIs. This indicates that the whole fibrogram could be used to improve HVI fiber length measurements across the cotton industry.
Lignin–Chitosan Nanocarriers for the Delivery of Bioactive Natural Products against Wood-Decay Phytopathogens
Authors: Sánchez-Hernández Eva , Langa-Lomba Natalia , González-García Vicente , Casanova-Gascón José , Martín-Gil Jesús , Santiago-Aliste Alberto , Torres-Sánchez Sergio , Martín-Ramos Pablo
Published: February 2022

The use of nanocarriers (NCs), i.e., nanomaterials capable of encapsulating drugs and releasing them selectively, is an emerging field in agriculture. In this study, the synthesis, characterization, and in vitro and in vivo testing of biodegradable NCs loaded with natural bioactive products was investigated for the control of certain phytopathogens responsible for wood degradation. In particular, NCs based on methacrylated lignin and chitosan oligomers, loaded with extracts from Rubia tinctorum, Silybum marianum, Equisetum arvense, and Urtica dioica, were first assayed in vitro against Neofusicoccum parvum, an aggressive fungus that causes cankers and diebacks in numerous woody hosts around the world. The in vitro antimicrobial activity of the most effective treatment was further explored against another fungal pathogen and two bacteria related to trunk diseases: Diplodia seriata, Xylophilus ampelinus, and Pseudomonas syringae pv. syringae, respectively. Subsequently, it was evaluated in field conditions, in which it was applied by endotherapy for the control of grapevine trunk diseases. In the in vitro mycelial growth inhibition tests, the NCs loaded with R. tinctorum resulted in EC90 concentrations of 65.8 and 91.0 μg·mL−1 against N. parvum and D. seriata, respectively. Concerning their antibacterial activity, a minimum inhibitory concentration of 37.5 μg·mL−1 was obtained for this treatment against both phytopathogens. Upon application via endotherapy on 20-year-old grapevines with clear esca and Botryosphaeria decay symptoms, no phytotoxicity effects were observed (according to SPAD and chlorophyll fluorescence measurements) and the sugar content of the grape juice was not affected either. Nonetheless, the treatment led to a noticeable decrease in foliar symptoms as well as a higher yield in the treated arms as compared to the control arms (3177 vs. 1932 g/arm), suggestive of high efficacy. Given the advantages in terms of controlled release and antimicrobial product savings, these biodegradable NCs loaded with natural extracts may deserve further research in large-scale field tests.
Consortia of Plant-Growth-Promoting Rhizobacteria Isolated from Halophytes Improve the Response of Swiss Chard to Soil Salinization
Authors: Redondo-Gómez Susana , Romano-Rodríguez Elena , Mesa-Marín Jennifer , Sola-Elías Cristina , Mateos-Naranjo Enrique
Published: February 2022

Inadequate fertilization or the indiscriminate use of water with high salt concentrations have led to salinization of agricultural soils. In this context, biofertilization with plant-growth-promoting rhizobacteria (PGPR) is an environmentally benign strategy to stimulate plant growth, even under salt stress. Thus, we studied the use of isolated PGPR consortia from halophytes to enhance Swiss chard growth under saline conditions. Growth, photosynthetic apparatus response, nutrient status, pigment concentrations, and secondary metabolites with antioxidant activity were determined in Swiss chard plants grown at 0 and 85 mmol L−1 NaCl. In general, inoculation of plants with PGPR has been shown to be an effective strategy to stimulate the growth of Swiss chard and improve its tolerance to salt stress. Inoculated plants watered with 85 mmol L−1 NaCl showed higher values of leaf dry weight than control plants. Furthermore, PGPR inoculation reduced electrolyte leakage and Na+ uptake and improved chlorophyll a fluorescence parameters, chlorophyll and carotenoid concentrations, stomatal conductance, and antioxidant capacity of Swiss chard. Finally, our findings highlight the potential of isolated PGPR from halophytes to counterbalance the deleterious effect of salinity and stimulate crop growth.
Detection of Methicillin-Resistant Staphylococcus aureus Infections Using Molecular Methods
Authors: Tenover Fred C. , Tickler Isabella A.
Published: February 2022

The application of molecular detection methods for bacterial pathogens has dramatically improved the outcomes of septic patients, including those with methicillin-resistant Staphylococcus aureus (MRSA) infections. Molecular methods can be applied to a variety of clinical specimens including nasal swabs, growth in blood culture bottles, and wounds. While data show that the overall accuracy of molecular tests for MRSA is high, results can be confounded by the presence of multiple staphylococcal species in a specimen, insertions and deletions of DNA in and around the Staphylococcal Cassette Chromosome mec (SCCmec) element, and point mutations in mecA. Herein, we explore the complexities of molecular approaches to MRSA detection and the instances where phenotypic methods should be pursued to resolve discrepancies between genotypic and phenotypic results.
Inappropriate Antibiotic Use in Zimbabwe in the COVID-19 Era: A Perfect Recipe for Antimicrobial Resistance
Authors: Chitungo Itai , Dzinamarira Tafadzwa , Nyazika Tinashe K. , Herrera Helena , Musuka Godfrey , Murewanhema Grant
Published: February 2022

The global COVID-19 pandemic has resulted in an upsurge in antimicrobial use. The increase in use is multifactorial, and is particularly related to the empirical treatment of SARS-CoV-2 and suspected coinfections with antimicrobials and the limited quality of diagnostics to differentiate viral and bacterial pneumonia. The lack of clear clinical guidelines across a wide range of settings, and the inadequacy of public health sectors in many countries, have contributed to this pattern. The increased use of antimicrobials has the potential to increase incidences of antimicrobial resistance, especially in low-resource countries such as Zimbabwe already grappling with multidrug-resistant micro-organism strains. By adopting the antimicrobial stewardship principles of the correct prescription and optimised use of antimicrobials, as well as diagnostic stewardship, revamping regulatory oversight of antimicrobial surveillance may help limit the occurrence of antimicrobial resistance during this pandemic.
Bio-Availability, Anticancer Potential, and Chemical Data of Lycopene: An Overview and Technological Prospecting
Authors: Amorim Adriany das Graças Nascimento , Vasconcelos Andreanne Gomes , Souza Jessica , Oliveira Ana , Gullón Beatriz , de Souza de Almeida Leite José Roberto , Pintado Manuela
Published: February 2022

The purpose of this review was to collect relevant chemical data about lycopene and its isomers, which can be extracted using different non-polar or polar aprotic solvents by SC-CO2 or biosynthesis as a friendly technique. Lycopene and other carotenoids can be identified and quantified by UV–Vis and HPLC using a C18 or C30 column, while their characterization is possible by UV–Vis, Fluorescence, FTIR, MS, NMR, and DSC assays. Among these techniques, the last four can compare lycopene isomers and identify cis or all-trans-lycopene. FTIR, MS, and NMR techniques are more suitable for the verification of the purity of lycopene extracts due to the signal complexity generated for each isomer, which enables identification by subtle differences. Additionally, some biological activities of lycopene isolated from red vegetables have already been confirmed, such as anti-inflammatory, antioxidant, and cytotoxic activity against cancer cells, probably by activating several pathways. The encapsulation of lycopene in nanoparticles demonstrated an improvement in oral delivery, and ex vivo assessments determined that these nanoparticles had better permeation and low cytotoxicity against human cells with enhanced permeation. These data suggest that lycopene has the potential to be applied in the food and pharmaceutical industries, as well as in cosmetic products.
Formulation of Gels and Emulgels with Malus domestica Borkh: Apple Extracts and Their Biopharmaceutical Evaluation In Vitro
Authors: Butkeviciute Aurita , Ramanauskiene Kristina , Janulis Valdimaras
Published: February 2022

Phenolic compounds that estimate apple extracts with multifaceted biological effects are potentially valuable for protection against skin disorders. The purpose of our research was to formulate gels and emulgels containing a complex of phenolic compounds of apple extracts and to perform a biopharmaceutical evaluation of semi-solid pharmaceutical forms, determining their antioxidant activity in vitro. HPLC analyses of phenolic compounds were performed. The total amount of phenolic compounds found in the sample of apples from the ‘Paprastasis antaninis’ cultivar was 1455.5 ± 72.8 µg/g. The release of phenolics from gels and emulgels was assessed by Franz-type diffusion cells. The in vitro release test revealed that phenolic compounds were released from the gel (G1–G6) formulations (70.6–73.8%) compared to the amounts (77.2–83.9%) released from the emulgel (E1–E6) formulations. The largest amount (83.9%) of phenolic compounds was released from the E5 formulation, while the smallest amounts (70.6%) were released from the formulations G3 and G5. The antioxidant activity evaluated by the DPPH and FRAP methods observed in all gel (G1–G6) and emulgel (E1–E6) formulations after 6 h were the strongest, compared to the activities observed in the formulations after 2 or 4 h. Gels and emulgels, which are rich in apple extracts, have strong antioxidant properties and may be promising choices for the development of new, innovative pharmaceutical forms or cosmetics.
Big Data for Traffic Estimation and Prediction: A Survey of Data and Tools
Authors: Jiang Weiwei , Luo Jiayun
Published: February 2022

Big data have been used widely in many areas, including the transportation industry. Using various data sources, traffic states can be well estimated and further predicted to improve the overall operation efficiency. Combined with this trend, this study presents an up-to-date survey of open data and big data tools used for traffic estimation and prediction. Different data types are categorized, and off-the-shelf tools are introduced. To further promote the use of big data for traffic estimation and prediction tasks, challenges and future directions are given for future studies.
Top-Down Detection of Eating Episodes by Analyzing Large Windows of Wrist Motion Using a Convolutional Neural Network
Authors: Sharma Surya , Hoover Adam
Published: February 2022

In this work, we describe a new method to detect periods of eating by tracking wrist motion during everyday life. Eating uses hand-to-mouth gestures for ingestion, each of which lasts a few seconds. Previous works have detected these gestures individually and then aggregated them to identify meals. The novelty of our approach is that we analyze a much longer window (0.5–15 min) using a convolutional neural network. Longer windows can contain other gestures related to eating, such as cutting or manipulating food, preparing foods for consumption, and resting between ingestion events. The context of these other gestures can improve the detection of periods of eating. We test our methods on the public Clemson all-day dataset, which consists of 354 recordings containing 1063 eating episodes. We found that accuracy at detecting eating increased by 15% in ≥4 min windows compared to ≤15 s windows. Using a 6 min window, we detected 89% of eating episodes, with 1.7 false positives for every true positive (FP/TP). These are the best results achieved to date on this dataset.
Biologically Inspired Optimal Terminal Iterative Learning Control for the Swing Phase of Gait in a Hybrid Neuroprosthesis: A Modeling Study
Authors: Makowski Nathaniel S. , Fitzpatrick Marshaun N. , Triolo Ronald J. , Reyes Ryan-David , Quinn Roger D. , Audu Musa
Published: February 2022

(1) Background: An iterative learning control (ILC) strategy was developed for a “Muscle First” Motor-Assisted Hybrid Neuroprosthesis (MAHNP). The MAHNP combines a backdrivable exoskeletal brace with neural stimulation technology to enable persons with paraplegia due to spinal cord injury (SCI) to execute ambulatory motions and walk upright. (2) Methods: The ILC strategy was developed to swing the legs in a biologically inspired ballistic fashion. It maximizes muscular recruitment and activates the motorized exoskeletal bracing to assist the motion as needed. The control algorithm was tested using an anatomically realistic three-dimensional musculoskeletal model of the lower leg and pelvis suitably modified to account for exoskeletal inertia. The model was developed and tested with the OpenSim biomechanical modeling suite. (3) Results: Preliminary data demonstrate the efficacy of the controller in swing-leg simulations and its ability to learn to balance muscular and motor contributions to improve performance and accomplish consistent stepping. In particular, the controller took 15 iterations to achieve the desired outcome with 0.3% error.
Effects of Advanced Age and Parkinson’s Disease on Joint-Level Kinetic Adaptations to Faster Walking Speeds
Authors: Kuhman Daniel , Kongsuk Jutaluk , Reed William R. , Rosenblatt Noah J. , Visscher Kristina , Walker Harrison , Hurt Christopher P.
Published: February 2022

Redistribution of mechanical output from the ankle to the hip during walking occurs with advanced age. Changes to tissues spanning the ankle may limit the joint from performing mechanical functions necessary to walk at fast speeds and older adults may redistribute work proximally to compensate. Older adults with Parkinson’s disease (PD) do not exhibit the distal-to-proximal redistribution and may therefore be limited in reaching fast walking speeds. We tested whether advanced aging, regardless of the presence of PD, limits the ability to increase motor-like behavior of the ankle as walking speed increases. We also tested whether healthy older adults—but not PD patients—would compensate for reduced motor-like behavior at the ankle with disproportionately larger mechanical redistributions at faster speeds. The 16 young, 16 older, and 8 PD-diagnosed adults walked at 0.8, 1.2, and 1.6 ms−1 on a treadmill. We used joint functional indexing to quantify motor-like behavior of the ankle and a hip-to-ankle mechanical work ratio to quantify mechanical redistribution. We found a significant group x speed interaction (p < 0.05) for motor-like behavior of the ankle, with younger adults increasing motor index more than the older and PD groups as walking speed increased. Contrary to our second hypothesis, we found a significant main effect of speed (p < 0.001) on redistribution ratios, indicating that all three groups decreased redistribution ratios as walking speed increased, but not a significant interaction.
Role of Microbiota-Gut-Brain Axis in Regulating Dopaminergic Signaling
Authors: Hamamah Sevag , Aghazarian Armin , Nazaryan Anthony , Hajnal Andras , Covasa Mihai
Published: February 2022

Dopamine is a neurotransmitter that plays a critical role both peripherally and centrally in vital functions such as cognition, reward, satiety, voluntary motor movements, pleasure, and motivation. Optimal dopamine bioavailability is essential for normal brain functioning and protection against the development of neurological diseases. Emerging evidence shows that gut microbiota have significant roles in maintaining adequate concentrations of dopamine via intricate, bidirectional communication known as the microbiota-gut-brain axis. The vagus nerve, immune system, hypothalamus–pituitary–adrenal axis, and microbial metabolites serve as important mediators of the reciprocal microbiota-gut-brain signaling. Furthermore, gut microbiota contain intrinsic enzymatic activity that is highly involved in dopamine metabolism, facilitating dopamine synthesis as well as its metabolite breakdown. This review examines the relationship between key genera of gut microbiota such as Prevotella, Bacteroides, Lactobacillus, Bifidobacterium, Clostridium,Enterococcus, and Ruminococcus and their effects on dopamine. The effects of gut dysbiosis on dopamine bioavailability and the subsequent impact on dopamine-related pathological conditions such as Parkinson’s disease are also discussed. Understanding the role of gut microbiota in modulating dopamine activity and bioavailability both in the periphery and in the central nervous system can help identify new therapeutic targets as well as optimize available methods to prevent, delay, or restore dopaminergic deficits in neurologic and metabolic disorders.