bluuebunny/crossref_metadata_2025 huggingface dataset:
Current Overview of Osteogenesis Imperfecta
Authors: Deguchi Mari , Tsuji Shunichiro , Katsura Daisuke , Kasahara Kyoko , Kimura Fuminori , Murakami Takashi
Published: May 2021

Osteogenesis imperfecta (OI), or brittle bone disease, is a heterogeneous disorder characterised by bone fragility, multiple fractures, bone deformity, and short stature. OI is a heterogeneous disorder primarily caused by mutations in the genes involved in the production of type 1 collagen. Severe OI is perinatally lethal, while mild OI can sometimes not be recognised until adulthood. Severe or lethal OI can usually be diagnosed using antenatal ultrasound and confirmed by various imaging modalities and genetic testing. The combination of imaging parameters obtained by ultrasound, computed tomography (CT), and magnetic resource imaging (MRI) can not only detect OI accurately but also predict lethality before birth. Moreover, genetic testing, either noninvasive or invasive, can further confirm the diagnosis prenatally. Early and precise diagnoses provide parents with more time to decide on reproductive options. The currently available postnatal treatments for OI are not curative, and individuals with severe OI suffer multiple fractures and bone deformities throughout their lives. In utero mesenchymal stem cell transplantation has been drawing attention as a promising therapy for severe OI, and a clinical trial to assess the safety and efficacy of cell therapy is currently ongoing. In the future, early diagnosis followed by in utero stem cell transplantation should be adopted as a new therapeutic option for severe OI.
Could DOAC Be a Better Choice Than Warfarin in Low Compliance Patients with Fontan Procedure?
Authors: Park Jisoo , Hong Bo-Young , Kim Joon-Sung , Park Jung-Geun , Jung Jiyoon , Lim Seong-Hoon
Published: May 2021

Background and Objectives: After the Fontan procedure, thromboembolic events need to be prevented. We present a young patient with a history of Fontan procedure and poor adherence to warfarin who developed systemic thromboembolism. He was changed to maintenance dabigatran, which is one of the available direct oral anticoagulants (DOACs). Case presentation: A 20-year-old man was diagnosed with cerebral infarct, pulmonary thromboembolism (PTE), and renal infarcts. He was prescribed warfarin to prevent thromboembolic events after the Fontan procedure. Based on his poor adherence to warfarin, we decided to change the anticoagulant therapy from warfarin to dabigatran 150 mg bid. One month later, his pulmonary thromboembolism regressed. Conclusion: Our case report showed a young adult with low compliance to warfarin who developed cerebral, pulmonary, and renal thromboembolic events. Thus, in our opinion, the change from warfarin to a DOAC was necessary for further prevention and treatment of PTE. A change from warfarin to a DOAC should be considered in patients with poor compliance who are at high risk of thromboembolic events, for example, after the Fontan procedure.
Mild Lactic Acid Stress Causes Strain-Dependent Reduction in SEC Protein Levels
Authors: Etter Danai , Jenni Céline , Tasara Taurai , Johler Sophia
Published: May 2021

Staphylococcal enterotoxin C (SEC) is a major cause of staphylococcal food poisoning in humans and plays a role in bovine mastitis. Staphylococcus aureus (S. aureus) benefits from a competitive growth advantage under stress conditions encountered in foods such as a low pH. Therefore, understanding the role of stressors such as lactic acid on SEC production is of pivotal relevance to food safety. However, stress-dependent cues and their effects on enterotoxin expression are still poorly understood. In this study, we used human and animal strains harboring different SEC variants in order to evaluate the influence of mild lactic acid stress (pH 6.0) on SEC expression both on transcriptional and translational level. Although only a modest decrease in sec mRNA levels was observed under lactic acid stress, protein levels showed a significant decrease in SEC levels for some strains. These findings indicate that post-transcriptional modifications can act in SEC expression under lactic acid stress.
The Modulation of NADPH Oxidase Activity in Human Neutrophils by Moroccan Strains of Leishmania major and Leishmania tropica Is Not Associated with p47phox Phosphorylation
Authors: Maksouri Hasnaa , Darif Dounia , Estaquier Jerome , Riyad Myriam , Desterke Christophe , Lemrani Meryem , Dang Pham My-Chan , Akarid Khadija
Published: May 2021

Polymorphonuclear neutrophils (PMNs) are the first phagocyte recruited and infected by Leishmania. They synthetize superoxide anions (O2−) under the control of the NADPH oxidase complex. In Morocco, Leishmania major and L. tropica are the main species responsible for cutaneous leishmaniasis (CL). The impact of these parasites on human PMN functions is still unclear. We evaluated the in vitro capacity of primary Moroccan strains of L. major and L. tropica to modulate PMN O2− production and p47phox phosphorylation status of the NADPH oxidase complex. PMNs were isolated from healthy blood donors, and their infection rate was measured by microscopy. O2− production was measured by superoxide dismutase–inhibitable reduction of cytochrome C. P47phox phosphorylation was analyzed by Western blot using specific antibodies against Ser328 and Ser345 sites. Whereas we did not observe any difference in PMN infectivity rate, our results indicated that only L. tropica promastigotes inhibited both fMLF- and PMA-mediated O2− production independently of p47phox phosphorylation. Leishmania soluble antigens (SLAs) from both species significantly inhibited O2− induced by fMLF or PMA. However, they only decreased PMA-induced p47phox phosphorylation. L. major and L. tropica modulated differently O2− production by human PMNs independently of p47phox phosphorylation. The inhibition of ROS production by L. tropica could be a mechanism of its survival within PMNs that might explain the reported chronic pathogenicity of L. tropica CL.
Micronutrients Deficiency, Supplementation and Novel Coronavirus Infections—A Systematic Review and Meta-Analysis
Authors: Wang Min Xian , Gwee Sylvia Xiao Wei , Pang Junxiong
Published: May 2021

Background: Micronutrients play roles in strengthening and maintaining immune function, but their supplementation and/or deficiency effects on respiratory tract infections are inconclusive. This review aims to systematically assess the associations between micronutrient supplementation or deficiency, with novel coronavirus incidence and disease severity. Methods: Systematic literature searches conducted in five electronic databases identified 751 unique studies, of which 33 studies (five supplementation studies, one supplementation and deficiency study, and 27 deficiency studies) were eventually included in this review. Proportions of incidence and severity outcomes in each group, and adjusted summary statistics with their relevant 95% confidence intervaIs (CI) were extracted. Data from 19 studies were pooled in meta-analysis using the generic inverse variance method. Findings: A total of 360,346 patients across 16 countries, with a mean age between 32 and 87.7 years, were involved across 33 studies. All studies were on COVID-19 infections. In individuals without micronutrient deficiency, there was a significant reduction on odds of COVID-19 incidence (pooled OR: 0.37, 95% CI: 0.18, 0.78), and ICU admissions or severe/critical disease onset when combined as a severity outcome (pooled OR: 0.26, 95% CI: 0.08, 0.89). Insignificant protective effects were observed on other outcome measures, namely mortality, ICU admission, progression to respiratory-related complications, severe/critical disease onset or requiring respiratory support and hospitalization rate. Conclusion: The absence of micronutrient deficiency significantly reduced COVID-19 incidence and clinical deterioration in hospitalized patients. Usage of micronutrients as prophylaxis and complementary supplement in therapeutic management of COVID-19 patients may be a promising and cost-effective approach warranting in-depth investigation.
Molecular Characterization of Ticks and Tick-Borne Pathogens in Cattle from Khartoum State and East Darfur State, Sudan
Authors: Mossaad Ehab , Gaithuma Alex , Mohamed Yassir O. , Suganuma Keisuke , Umemiya-Shirafuji Rika , Ohari Yuma , Salim Bashir , Liu Mingming , Xuan Xuenan
Published: May 2021

Ticks transmit many pathogens with public health and veterinary importance. Despite the wide distribution of tick-borne pathogens in Sudan, the information on the tick–pathogen relationship needs to be updated, particularly using modern molecular techniques. This cross-sectional study, conducted between September and November 2019, used morphology, PCR, and sequencing to confirm the identity of adult cattle ticks (male and female; n = 536) from Khartoum State (n = 417) and East Darfur State (n = 119). Moreover, the presence of Theileria annulata, Babesia bigemina, B. bovis, Anaplasma marginale, and Ehrlichia ruminantium was detected and confirmed in each tick using species-specific PCR or nested PCR and sequencing. The most economically important tick genera, Rhipicephalus, Hyalomma, and Amblyomma, were prevalent in the study area, and 13 different tick species were identified. The most prevalent tick species were Rhipicephalusevertsi evertsi (34.3%) and Hyalomma anatolicum (57.3%) in Khartoum State, and Rhipicephalus annulatus (27%), Rhipicephalus decoloratus (25%), and Hyalomma rufipes (29%) in East Darfur State. We detected all five pathogens in both states. To the best of our knowledge, this is the first study to report the presence of E. ruminantium, its vector Amblyomma variegatum, and B. bovis in Khartoum State. Further, this is the first report on most tick and pathogen species identified in East Darfur State. Our findings indicate the migration of some tick and pathogen species beyond their distribution areas in the country, and this consideration is necessary to develop future control strategies.
Cymbopogon winterianus Essential Oil Attenuates Bleomycin-Induced Pulmonary Fibrosis in a Murine Model
Authors: Tavares Lívia A. , Rezende Allan A. , Santos Jymmys L. , Estevam Charles S. , Silva Ana M. O. , Schneider Jaderson K. , Cunha John L. S. , Droppa-Almeida Daniela , Correia-Neto Ivan J. , Cardoso Juliana C. , Severino Patricia , Souto Eliana B. , de Albuquerque-Júnior Ricardo L. C.
Published: May 2021

The essential oil of Cymbopogon winterianus (EOCW) is a natural product with antioxidant, anti-inflammatory, and antifibrotic properties. We studied the effect of EOCW in the progression of histological changes of pulmonary fibrosis (PF) in a rodent model. The oil was obtained by hydrodistillation and characterized using gas chromatography–mass spectrometry. Intratracheal instillation of bleomycin was performed in 30 rats to induce PF, while Sham animals were subjected to instillation of saline solution. The treatment was performed using daily oral administration of distilled water, EOCW at 50, 100, and 200 mg/kg, and deflazacort (DFC). After 28 days, hemogram and bronchoalveolar lavage fluid (BALF), tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were assayed. Histological grading of PF, immunohistochemical expression of α-smooth muscle actin (α-SMA), and transforming growth factor-β (TGF-β) were also analyzed. The EOCW major compounds were found to be citronellal, geraniol, and citronellol. EOCW significantly reduced inflammation in BALF, reduced MDA levels, and increased SOD activity. EOCW attenuated histological grading of PF and reduced immunohistochemical expression of α-SMA and TGF-β in a dose-dependent way, likely due to the reduction of oxidative stress, inflammation, and TGF-β-induced myofibroblast differentiation.
Constituents of Chamaecrista diphylla (L.) Greene Leaves with Potent Antioxidant Capacity: A Feature-Based Molecular Network Dereplication Approach
Authors: Gomes Paulo , Quirós-Guerrero Luis , Muribeca Abraão , Reis José , Pamplona Sônia , Lima Anderson , Trindade Mariele , Silva Consuelo , Souza Jesus , Boutin Jean , Wolfender Jean-Luc , Silva Milton
Published: May 2021

Chamaecrista diphylla (L.) Greene (Fabaceae/Caesalpiniaceae) is a herbaceous plant that is widely distributed throughout the Americas. Plants from this genus have been used in traditional medicine as a laxative, to heal wounds, and to treat ulcers, snake and scorpion bites. In the present study, we investigated the chemical composition of Chamaecrista diphylla leaves through a mass spectrometry molecular network approach. The oxygen radical absorbance capacity (ORAC) for the ethanolic extract, enriched fractions and isolated compounds was assessed. Overall, thirty-five compounds were annotated for the first time in C. diphylla. Thirty-two of them were reported for the first time in the genus. The isolated compounds 9, 12, 24 and 33 showed an excellent antioxidant capacity, superior to the extract and enriched fractions. Bond dissociation energy calculations were performed to explain and sustain the antioxidant capacity found. According to our results, the leaves of C. diphylla represent a promising source of potent antioxidant compounds.
Image Analysis: A Versatile Tool in the Manufacturing and Quality Control of Pharmaceutical Dosage Forms
Authors: Farkas Dóra , Madarász Lajos , Nagy Zsombor K. , Antal István , Kállai-Szabó Nikolett
Published: May 2021

In pharmaceutical sciences, visual inspection is one of the oldest methods used for description in pharmacopeias and is still an important part of the characterization and qualification of active ingredients, excipients, and dosage forms. With the development of technology, it is now also possible to take images of various pharmaceutical dosage forms with different imaging methods in a size range that is hardly visible or completely invisible to the human eye. By analyzing high-quality designs, physicochemical processes can be understood, and the results can be used even in the optimization of the composition of the dosage form and in the development of its production. The present study aims to show some of the countless ways image analysis can be used in the manufacturing and quality assessment of different dosage forms. This summary also includes measurements and an evaluation of, amongst others, a less studied dosage form, medicated foams.
Constraining Dense Matter Physics Using f-Mode Oscillations in Neutron Stars
Authors: Jaiswal Sukrit , Chatterjee Debarati
Published: May 2021

In this paper, an investigation of the role of nuclear saturation parameters on f-mode oscillations in neutron stars is performed within the Cowling approximation. It is found that the uncertainty in the effective nucleon mass plays a dominant role in controlling the f-mode frequencies. The effect of the uncertainties in saturation parameters on previously-proposed empirical relations of the frequencies with astrophysical observables relevant for asteroseismology are also investigated. These results can serve as an important tool for constraining the nuclear parameter space and understand the behaviour of dense nuclear matter from the future detection of f-modes.
Metabolic Analysis of Root, Stem, and Leaf of Scutellaria baicalensis Plantlets Treated with Different LED Lights
Authors: Yeo Hyeon-Ji , Park Chang-Ha , Park Soo-Yun , Chung Sun-Ok , Kim Jae-Kwang , Park Sang-Un
Published: May 2021

Light emitting diodes (LEDs) have recently been considered an efficient artificial light source in plant factories for enhancing plant growth and nutritional quality. Accordingly, this study aimed to review blue, red, and white LED light sources for efficiency and length of the growing period to produce seedlings of Scutellaria baicalensis with high nutritional value. The roots, stems, and leaves of S. baicalensis seedlings were grown under different LED lights and harvested after two and four weeks, and analyzed using high-performance liquid chromatography and gas chromatography time-of-flight mass spectrometry to identify and quantify primary and secondary metabolites. Roots, particularly in the seedlings treated with white LEDs were determined to contain the greatest concentrations of the representative compounds present in S. baicalensis: baicalin, baicalein, and wogonin, which show highly strong biological properties compared to the other plant organs. A total of 50 metabolites (amino acids, sugars, sugar alcohols, organic acids, phenolic acids, and amines) were detected in the roots, stems, and leaves of S. baicalensis seedlings, and the concentrations of primary and secondary metabolites were generally decreased with the increasing duration of LED illumination. Therefore, this study suggests that white LED light and a 2-week growing period are the most efficient conditions for the production of baicalin, baicalein, and wogonin.
Influence of Land Use on the C and N Status of a C4 Invasive Grass in a Semi-Arid Region: Implications for Biomonitoring
Authors: Díaz-Álvarez Edison A. , de la Barrera Erick
Published: May 2021

Biomonitoring of atmospheric pollution is an increasingly accepted practice. However, most existing biomonitors are usually epiphytic species from mesic environments. This work assessed the suitability of buffelgrass (Cenchrus ciliaris), an invasive C4 grass in northwestern Mexico, as a biomonitor, by means of the spatial distribution of the carbon and nitrogen content and isotopic signatures for grass samples collected from urban, agricultural, and natural areas throughout the state of Sonora. We found the highest tissue carbon content of 45.6% (on a dry weight basis) and highest nitrogen content of 3.31% for buffelgrass from the Yaqui Valley. We also found the lowest δ13C of −15.9‰, and the highest δ15N of 16.7‰ in the same region. In contrast, the lowest carbon and nitrogen content of 39.4 and 1.49% were found for Bahía de Kino and Río Sonora mountains, respectively. The lowest δ15N of 2.18‰ and the highest δ13C of −13.7‰ were measured for two remote locations. These results show the influence that pollutant emissions, including agriculture and transportation, have on elemental and isotopic composition of vegetation. Buffelgrass is most adequate for tracking carbon and nitrogen emissions in arid environments and for determining alterations on nitrogen soil reactions, as a first approximation for saturation.
Red Raspberry (Rubus idaeus L.) Seed Oil: A Review
Authors: Ispiryan Audronė , Viškelis Jonas , Viškelis Pranas
Published: May 2021

Raspberry (Rubus idaeus L.) seed oil (RSO) is considered as a source of high value bioactive compounds as fatty acids, tocopherols, tocotrienols, carotenoids, flavonoids, phytosterols, antioxidants, monoterpenes and many other chemical constituents. These compounds are appreciated as a source of nutrition for humans, as additives in cosmetic production, has immense therapeutic potential. Raspberry seed oil exerts many pharmacological effects included antimicrobial, antioxidant, anti-inflammatory activity and many other effects. The various databases like PubMed and Science Direct were used to identify, analyze and summarize the research literature on raspberries. This review will highlight recent developments of the chemical constituents and nutraceutical and cosmetical effects of RSO. Practical application: analyzed recent researches and international patents containing raspberry seed oil can help practitioners of various industries create new high-value products.
Anti-Osteoporotic Effects of Commiphora Myrrha and Its Poly-Saccharide via Osteoclastogenesis Inhibition
Authors: Hwang Youn-Hwan , Lee Ami , Kim Taesoo , Jang Seon-A , Ha Hyunil
Published: May 2021

In traditional oriental medicines, Commiphora myrrha and its resinous exudate (i.e., myrrh) are used as herbal remedies to treat various inflammatory and metabolic disorders. Until now, C. myrrha-derived herbal products are considered useful source for bioactive compounds to manage numerous human diseases. This study investigated the effects of water extract of C. myrrha resin (WCM) and its polysaccharide (WCM-PE) on modulatory effects of osteoclast differentiation and/or ovariectomized-induced bone loss. Oral administration of WCM (200 and 500 mg/kg/day for four weeks) notably decreased trabecular bone loss and lipid accumulation in the bone marrow cavity. WCM and WCM-PE dose-dependently inhibited receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis and suppressed RANKL-mediated overexpression of c-Fos and nuclear factor of activated T cells, cytoplasmic 1, thereby downregulating osteoclast-specific gene (Atp6v0d2, DC-STAMP and cathepsin K) expression. Thus, our results suggest that WCM and WCM-PE are promising nutraceutical candidates for the management of osteoporosis in postmenopausal women.
Inhibitory Effects of Gyeji-Tang on MMP-9 Activity and the Expression of Adhesion Molecules in IL-4- and TNF-α-Stimulated BEAS-2B Cells
Authors: Kim Yu Jin , Jeon Woo-Young , Hwang Youn-Hwan , Lee Mee-Young
Published: May 2021

Gyeji-tang (GJT), a traditional herbal formula composed of five herbal medicines, is commonly used to treat the common cold, exogenous febrile disease, fever and headaches in Korea, China and Japan. Although various pharmacological activities of GJT have been reported in several studies, the effect of GJT water extract (GJTWE) on airway inflammation has not yet been investigated. This study aimed to evaluate the effects of GJTWE on airway inflammation-related factors using human bronchial epithelial BEAS-2B cells, and to identify the phytochemicals in GJTWE by ultra-performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MS/MS) analysis. GJTWE significantly decreased the production of chemokines, including eotaxin-3, eotaxin-1, regulated on activation normal T-cell expressed and secreted (RANTES), and matrix metalloproteinase-9, and the expression of the adhesion molecules, intercellular adhesion molecule-1 and vascular cell adhesion molecule-1, in interleukin-4 + tumor necrosis factor-α (IT)-stimulated BEAS-2B cells. In the UPLC-DAD-MS/MS analysis, 21 phytochemicals, including six flavonoids, two chalcones, five terpenoids, six phenolics, one phenylpropanoid and one coumarin, were identified in GJTWE. The findings suggested that GJTWE might exhibit anti-inflammatory effects on airway inflammation by regulating the expression of inflammatory response-related factors in IT-stimulated BEAS-2B cells; further studies are required to determine the bioactive compounds involved in the inhibition of airway inflammation.
Dissipative Particle Dynamics Study on Interfacial Properties of Symmetric Ternary Polymeric Blends
Authors: Liu Dongmei , Gong Kai , Lin Ye , Liu Tao , Liu Yu , Duan Xiaozheng
Published: May 2021

We investigated the interfacial properties of symmetric ternary An/AmBm/Bn and An/Am/2BmAm/2/Bn polymeric blends by means of dissipative particle dynamics (DPD) simulations. We systematically analyzed the effects of composition, chain length, and concentration of the copolymers on the interfacial tensions, interfacial widths, and the structures of each polymer component in the blends. Our simulations show that: (i) the efficiency of the copolymers in reducing the interfacial tension is highly dependent on their compositions. The triblock copolymers are more effective in reducing the interfacial tension compared to that of the diblock copolymers at the same chain length and concentration; (ii) the interfacial tension of the blends increases with increases in the triblock copolymer chain length, which indicates that the triblock copolymers with a shorter chain length exhibit a better performance as the compatibilizers compared to that of their counterparts with longer chain lengths; and (iii) elevating the triblock copolymer concentration can promote copolymer enrichment at the center of the interface, which enlarges the width of the phase interfaces and reduces the interfacial tension. These findings illustrate the correlations between the efficiency of copolymer compatibilizers and their detailed molecular parameters.
Synergistic Antimicrobial Activities of Thai Household Essential Oils in Chitosan Film
Authors: Tantala Juthamas , Rachtanapun Pornchai , Rachtanapun Chitsiri
Published: May 2021

Foodborne pathogens mostly contaminate ready-to-eat (RTE) meat products by post-process contamination and cause foodborne disease outbreaks. Preventing post-process contamination and controlling microbial growth during storage by packing the RTE meats with active antimicrobial film from chitosan combined with the synergism of Thai household essential oils was investigated. Here, we analyzed antimicrobial activity and mechanical properties of chitosan films incorporated with essential oil of fingerroot (EOF) and holy basil (EOH) based on their fractional inhibitory concentration and isobolograms. We showed that antimicrobial activities of chitosan film and chitosan films formulated with EOF:EOH displayed a dramatical reduction of Listeria monocytogenes Scott A concentration by 7 Log in 12 h. Chitosan film incorporated with EOF:EOH at ratio 0.04:0.04% v/v/w strongly retarded growth of total viable count of L. monocytogenes on vacuum-packed bologna slices during seven days of storage at 4 and 10 °C. Combined EOF and EOH added to chitosan films did not alter thickness, elongation (%) and colors (L*, a* and b*) of the chitosan film, but it increased water vapor transmission rate and decreased film tensile strength. Results suggested that chitosan film had strong antibacterial properties. Its effectiveness in inhibiting foodborne pathogenic bacteria in ready-to-eat meat products was enhanced by adding a combination of EOF:EOH.
Tailored Adhesion Properties of Acrylate Adhesives on Al Alloys by the Addition of Mn-Al–LDH
Authors: Tomić Natasa Z. , Saleh Mohamed Nasr , Vuksanović Marija M. , Egelja Adela , Obradović Vera , Marinković Aleksandar , Jančić Heinemann Radmila
Published: May 2021

The goal of this study was to investigate the effect of the structure of Mn-Al layered double hydroxide (LDH) on the adhesion behavior of composite adhesives on two Al alloys (L3005 and L8079). The composite adhesives were made out of the UV-curing Bisphenol A glycidylmethacrylate/triethylene glycol dimethacrylate (BT) as polymer matrix and the addition of 1, 3, and 5 wt. % of Mn-Al LDH as adhesion enhancers. Adhesion was evaluated by using the micro Vickers hardness testing procedure. The wetting angle of composite adhesives to the Al substrates was measured and compared to the adhesion parameter b obtained from the microhardness tests. The highest increase in adhesion was observed for BT with 5 wt. % of Mn-Al LDH on L3005 substrate, which was more than 15 times higher than the adhesion for the neat BT. The morphological segregation of composite adhesives after the contact with Al substrates was examined by optical microscopy and a higher compatibility of Mn-Al LDH particles with L3005 substrate was found. The methods used for the adhesion properties assessment suggested that the Mn-Al LDH was the best adhesion enhancer of the BT matrix for L3005 substrate containing a higher content of Mn and surface hydroxyl groups.
Engineering of Optimized Hydrogel Formulations for Cartilage Repair
Authors: Fu Yao , Zoetebier Bram , Both Sanne , Dijkstra Pieter J. , Karperien Marcel
Published: May 2021

The ideal scaffold for cartilage regeneration is expected to provide adequate mechanical strength, controlled degradability, adhesion, and integration with the surrounding native tissue. As it does this, it mimics natural ECMs functions, which allow for nutrient diffusion and promote cell survival and differentiation. Injectable hydrogels based on tyramine (TA)-functionalized hyaluronic acid (HA) and dextran (Dex) are a promising approach for cartilage regeneration. The properties of the hydrogels used in this study were adjusted by varying polymer concentrations and ratios. To investigate the changes in properties and their effects on cellular behavior and cartilage matrix formation, different ratios of HA- and dextran-based hybrid hydrogels at both 5 and 10% w/v were prepared using a designed mold to control generation. The results indicated that the incorporation of chondrocytes in the hydrogels decreased their mechanical properties. However, rheological and compression analysis indicated that 5% w/v hydrogels laden with cells exhibit a significant increase in mechanical properties after 21 days when the constructs are cultured in a chondrogenic differentiation medium. Moreover, compared to the 10% w/v hydrogels, the 5% w/v hybrid hydrogels increased the deposition of the cartilage matrix, especially in constructs with a higher Dex–TA content. These results indicated that 5% w/v hybrid hydrogels with 25% HA–TA and 75% Dex–TA have a high potential as injectable scaffolds for cartilage tissue regeneration.
Synthesis, Characterization, and Electrospinning of a Functionalizable, Polycaprolactone-Based Polyurethane for Soft Tissue Engineering
Authors: Hu Jin-Jia , Liu Chia-Chi , Lin Chih-Hsun , Tuan-Mu Ho-Yi
Published: May 2021

We synthesized a biodegradable, elastomeric, and functionalizable polyurethane (PU) that can be electrospun for use as a scaffold in soft tissue engineering. The PU was synthesized from polycaprolactone diol, hexamethylene diisocyanate, and dimethylolpropionic acid (DMPA) chain extender using two-step polymerization and designated as PU-DMPA. A control PU using 1,4-butanediol (1,4-BDO) as a chain extender was synthesized similarly and designated as PU-BDO. The chemical structure of the two PUs was verified by FT-IR and 1H-NMR. The PU-DMPA had a lower molecular weight than the PU-BDO (~16,700 Da vs. ~78,600 Da). The melting enthalpy of the PU-DMPA was greater than that of the PU-BDO. Both the PUs exhibited elastomeric behaviors with a comparable elongation at break (λ=L/L0= 13.2). The PU-DMPA had a higher initial modulus (19.8 MPa vs. 8.7 MPa) and a lower linear modulus (0.7 MPa vs. 1.2 MPa) and ultimate strength (9.5 MPa vs. 13.8 MPa) than the PU-BDO. The PU-DMPA had better hydrophilicity than the PU-BDO. Both the PUs displayed no cytotoxicity, although the adhesion of human umbilical artery smooth muscle cells on the PU-DMPA surface was better. Bead free electrospun PU-DMPA membranes with a narrow fiber diameter distribution were successfully fabricated. As a demonstration of its functionalizability, gelatin was conjugated to the electrospun PU-DMPA membrane using carbodiimide chemistry. Moreover, hyaluronic acid was immobilized on the amino-functionalized PU-DMPA. In conclusion, the PU-DMPA has the potential to be used as a scaffold material for soft tissue engineering.
Variables Governing Photosynthesis and Growth in Microalgae Mass Cultures
Authors: Masojídek Jiří , Ranglová Karolína , Lakatos Gergely Ernö , Silva Benavides Ana Margarita , Torzillo Giuseppe
Published: May 2021

Since the 1950s, microalgae have been grown commercially in man-made cultivation units and used for biomass production as a source of food and feed supplements, pharmaceuticals, cosmetics and lately biofuels, as well as a means for wastewater treatment and mitigation of atmospheric CO2 build-up. In this work, photosynthesis and growth affecting variables—light intensity, pH, CO2/O2 exchange, nutrient supply, culture turbulence, light/dark cell cycling, biomass density and culture depth (light path)—are reviewed as concerns in microalgae mass cultures. Various photosynthesis monitoring techniques were employed to study photosynthetic performance to optimize the growth of microalgae strains in outdoor cultivation units. The most operative and reliable techniques appeared to be fast-response ones based on chlorophyll fluorescence and oxygen production monitoring, which provide analogous results.
Actuator Saturated Fuzzy Controller Design for Interval Type-2 Takagi-Sugeno Fuzzy Models with Multiplicative Noises
Authors: Chang Wen-Jer , Lin Yu-Wei , Lin Yann-Horng , Pen Chin-Lin , Tsai Ming-Hsuan
Published: May 2021

In many practical systems, stochastic behaviors usually occur and need to be considered in the controller design. To ensure the system performance under the effect of stochastic behaviors, the controller may become bigger even beyond the capacity of practical applications. Therefore, the actuator saturation problem also must be considered in the controller design. The type-2 Takagi-Sugeno (T-S) fuzzy model can describe the parameter uncertainties more completely than the type-1 T-S fuzzy model for a class of nonlinear systems. A fuzzy controller design method is proposed in this paper based on the Interval Type-2 (IT2) T-S fuzzy model for stochastic nonlinear systems subject to actuator saturation. The stability analysis and some corresponding sufficient conditions for the IT2 T-S fuzzy model are developed using Lyapunov theory. Via transferring the stability and control problem into Linear Matrix Inequality (LMI) problem, the proposed fuzzy control problem can be solved by the convex optimization algorithm. Finally, a nonlinear ship steering system is considered in the simulations to verify the feasibility and efficiency of the proposed fuzzy controller design method.
Upgrading of Coffee Biocrude Oil Produced by Pyrolysis of Spent Coffee Grounds: Behavior of Fatty Acids in Supercritical Ethanol Reaction and Catalytic Cracking
Authors: Park Ji-Yeon , Kanak Md Amirul Alam , Lee In-Gu
Published: May 2021

Spent coffee grounds contain lipids (fatty acids) in addition to cellulose, hemicellulose, and lignin. The reaction process for upgrading biocrude oil produced from spent coffee grounds is different from that followed for upgrading biomass pyrolysis oil, such as processes that utilize sawdust. The feasibility of upgrading coffee biocrude oil through a supercritical ethanol reaction with plastic pyrolysis oil and through catalytic cracking for the improvement of the undesirable properties of biocrude oil, caused by the presence of oxygenated compounds, was evaluated. The initial oxygen content of the coffee biocrude oil was 16.9 wt%. The oil comprised a total content of 40.9% fatty acids, as found by analyzing the GC-MS peak area. After the supercritical ethanol reaction at 340 ∘C, the oxygen content was decreased to 9.9 wt%. When the MgNiMo/AC catalyst was applied to the supercritical reaction, the oxygen content was further decreased to 8.5 wt%. The esterification of the fatty acids in the biocrude oil with ethanol converted them to esters. After the supercritical reaction of coffee biocrude oil with plastic pyrolysis oil (1:2 (w/w)), the oxygen content was 6.4 wt%. After the catalytic cracking of the biocrude oil by Ni/MCM-41 at 400 ∘C, the fatty acids were converted to hydrocarbons, C9 to C21, and the oxygen content decreased to a final value of 2.8 wt%.
Assessment of the Accumulation Ability of Festuca rubra L. and Alyssum saxatile L. Tested on Soils Contaminated with Zn, Cd, Ni, Pb, Cr, and Cu
Authors: Pusz Agnieszka , Wiśniewska Magdalena , Rogalski Dominik
Published: May 2021

The contamination of soils with metals applies, in particular, to areas related to industry, the mining of raw materials and ores, transport, and agriculture. Unlike organic materials, metals cannot degrade over time and need to be reduced, removed, or immobilized in soil. One of the remediation methods for soils contaminated with metals is phytoextraction, which uses plants’ ability to accumulate metals in their own tissues. Metals enter the plant organism through the roots and are transported to the aboveground parts, where they are accumulated. In this study, we evaluated the phytoaccumulative abilities of two plant species tested on soils from industrial areas contaminated with metals to different extents (Zn, Cd, Ni, Pb, Cr, and Cu). The research was conducted for three years under the conditions of a pot experiment. In order to obtain four soils with varying degrees of metal contamination, two soils from industrial areas, G1 (contaminated) and G2 (uncontaminated), were mixed in the following ratios: 1:1, 1:3, 1:7, and 1:9. In the phytoremediation process, Festuca rubra L. and Alyssum saxatile L. were tested. After analyzing the results of the bioconcentration factor (BCF) for the tested plants, it was noted that both of the tested plants accumulated Cd and Zn more easily, followed by Cu, Ni, and Cr, and then Pb to a lesser extent. The values of factors for Cd and Zn were correlated with the high mobility of these elements compared to other metals and their relatively easy uptake by plants. Alyssum saxatile L. has an ability to accumulate Cd compared to Festuca rubra L., which is confirmed by the BCF (0.764) and transfer factor (TF) (3.5) (for 1:7 combination) values. The calculated results for the BCFs for Alyssum saxatile L. are less than one for all tested metals, which allows us to state that Alyssum saxatile L. is not an accumulator.
Distribution and Attribution of Terrestrial Snow Cover Phenology Changes over the Northern Hemisphere during 2001–2020
Authors: Chen Xiaona , Yang Yaping , Ma Yingzhao , Li Huan
Published: May 2021

Snow cover phenology has exhibited dramatic changes in the past decades. However, the distribution and attribution of the hemispheric scale snow cover phenology anomalies remain unclear. Using satellite-retrieved snow cover products, ground observations, and reanalysis climate variables, this study explored the distribution and attribution of snow onset date, snow end date, and snow duration days over the Northern Hemisphere from 2001 to 2020. The latitudinal and altitudinal distributions of the 20-year averaged snow onset date, snow end date, and snow duration days are well represented by satellite-retrieved snow cover phenology matrixes. The validation results by using 850 ground snow stations demonstrated that satellite-retrieved snow cover phenology matrixes capture the spatial variability of the snow onset date, snow end date, and snow duration days at the 95% significance level during the overlapping period of 2001–2017. Moreover, a delayed snow onset date and an earlier snow end date (1.12 days decade−1, p < 0.05) are detected over the Northern Hemisphere during 2001–2020 based on the satellite-retrieved snow cover phenology matrixes. In addition, the attribution analysis indicated that snow end date dominates snow cover phenology changes and that an increased melting season temperature is the key driving factor of snow end date anomalies over the NH during 2001–2020. These results are helpful in understanding recent snow cover change and can contribute to climate projection studies.
Terrain Proxy-Based Site Classification for Seismic Zonation in North Korea within a Geospatial Data-Driven Workflow
Authors: Kim Han-Saem , Sun Chang-Guk , Lee Moon-Gyo , Cho Hyung-Ik
Published: May 2021

Numerous seismic activities occur in North Korea. However, it is difficult to perform seismic hazard assessment and obtain zonal data in the Korean Peninsula, including North Korea, when applying parametric or nonparametric methods. Remote sensing can be implemented for soil characterization or spatial zonation studies on irregular, surficial, and subsurface systems of inaccessible areas. Herein, a data-driven workflow for extracting the principal features using a digital terrain model (DTM) is proposed. In addition, geospatial grid information containing terrain features and the average shear wave velocity in the top 30 m of the subsurface (VS30) are employed using geostatistical interpolation methods; machine learning (ML)-based regression models were optimized and VS30-based seismic zonation in the test areas in North Korea were forecasted. The interrelationships between VS30 and terrain proxy (elevation, slope, and landform class) in the training area in South Korea were verified to define the input layer in regression models. The landform class represents a new proxy of VS30 and was subgrouped according to the correlation with grid-based VS30. The geospatial grid information was generated via the optimum geostatistical interpolation method (i.e., sequential Gaussian simulation (SGS)). The best-fitting model among four ML methods was determined by evaluating cost function-based prediction performance, performing uncertainty analysis for the empirical correlations of VS30, and studying spatial correspondence with the borehole-based VS30 map. Subsequently, the best-fitting regression models were designed by training the geospatial grid in South Korea. Then, DTM and its terrain features were constructed along with VS30 maps for three major cities (Pyongyang, Kaesong, and Nampo) in North Korea. A similar distribution of the VS30 grid obtained using SGS was shown in the multilayer perceptron-based VS30 map.
Quantifying the Response of German Forests to Drought Events via Satellite Imagery
Authors: Philipp Marius , Wegmann Martin , Kübert-Flock Carina
Published: May 2021

Forest systems provide crucial ecosystem functions to our environment, such as balancing carbon stocks and influencing the local, regional and global climate. A trend towards an increasing frequency of climate change induced extreme weather events, including drought, is hereby a major challenge for forest management. Within this context, the application of remote sensing data provides a powerful means for fast, operational and inexpensive investigations over large spatial scales and time. This study was dedicated to explore the potential of satellite data in combination with harmonic analyses for quantifying the vegetation response to drought events in German forests. The harmonic modelling method was compared with a z-score standardization approach and correlated against both, meteorological and topographical data. Optical satellite imagery from Landsat and the Moderate Resolution Imaging Spectroradiometer (MODIS) was used in combination with three commonly applied vegetation indices. Highest correlation scores based on the harmonic modelling technique were computed for the 6th harmonic degree. MODIS imagery in combination with the Normalized Difference Vegetation Index (NDVI) generated hereby best results for measuring spectral response to drought conditions. Strongest correlation between remote sensing data and meteorological measures were observed for soil moisture and the self-calibrated Palmer Drought Severity Index (scPDSI). Furthermore, forests regions over sandy soils with pine as the dominant tree type were identified to be particularly vulnerable to drought. In addition, topographical analyses suggested mitigated drought affects along hill slopes. While the proposed approaches provide valuable information about vegetation dynamics as a response to meteorological weather conditions, standardized in-situ measurements over larger spatial scales and related to drought quantification are required for further in-depth quality assessment of the used methods and data.
Towards the Spectral Mapping of Plastic Debris on Beaches
Authors: Guffogg Jenna A. , Soto-Berelov Mariela , Jones Simon D. , Bellman Chris J. , Lavers Jennifer L. , Skidmore Andrew K.
Published: May 2021

Floating and washed ashore marine plastic debris (MPD) is a growing environmental challenge. It has become evident that secluded locations including the Arctic, Antarctic, and remote islands are being impacted by plastic pollution generated thousands of kilometers away. Optical remote sensing of MPD is an emerging field that can aid in monitoring remote environments where in-person observation and data collection is not always feasible. Here we evaluate MPD spectral features in the visible to shortwave infrared regions for detecting varying quantities of MPD that have accumulated on beaches using a spectroradiometer. Measurements were taken from a range of in situ MPD accumulations ranging from 0.08% to 7.94% surface coverage. Our results suggest that spectral absorption features at 1215 nm and 1732 nm are useful for detecting varying abundance levels of MPD in a complex natural environment, however other absorption features at 931 nm, 1045 nm and 2046 nm could not detect in situ MPD. The reflectance of some in situ MPD accumulations was statistically different from samples that only contained organic debris and sand between 1.56% and 7.94% surface cover; however other samples with similar surface cover did not have reflectance that was statistically different from samples containing no MPD. Despite MPD being detectable against a background of sand and organic beach debris, a clear relationship between the surface cover of MPD and the strength of key absorption features could not be established. Additional research is needed to advance our understanding of the factors, such as type of MPD assemblage, that contribute to the bulk reflectance of MPD contaminated landscapes.
Small Object Detection in Remote Sensing Images with Residual Feature Aggregation-Based Super-Resolution and Object Detector Network
Authors: Bashir Syed Muhammad Arsalan , Wang Yi
Published: May 2021

This paper deals with detecting small objects in remote sensing images from satellites or any aerial vehicle by utilizing the concept of image super-resolution for image resolution enhancement using a deep-learning-based detection method. This paper provides a rationale for image super-resolution for small objects by improving the current super-resolution (SR) framework by incorporating a cyclic generative adversarial network (GAN) and residual feature aggregation (RFA) to improve detection performance. The novelty of the method is threefold: first, a framework is proposed, independent of the final object detector used in research, i.e., YOLOv3 could be replaced with Faster R-CNN or any object detector to perform object detection; second, a residual feature aggregation network was used in the generator, which significantly improved the detection performance as the RFA network detected complex features; and third, the whole network was transformed into a cyclic GAN. The image super-resolution cyclic GAN with RFA and YOLO as the detection network is termed as SRCGAN-RFA-YOLO, which is compared with the detection accuracies of other methods. Rigorous experiments on both satellite images and aerial images (ISPRS Potsdam, VAID, and Draper Satellite Image Chronology datasets) were performed, and the results showed that the detection performance increased by using super-resolution methods for spatial resolution enhancement; for an IoU of 0.10, AP of 0.7867 was achieved for a scale factor of 16.
Rechained: Sybil-Resistant Distributed Identities for the Internet of Things and Mobile Ad Hoc Networks
Authors: Bochem Arne , Leiding Benjamin
Published: May 2021

Today, increasing Internet of Things devices are deployed, and the field of applications for decentralized, self-organizing networks keeps growing. The growth also makes these systems more attractive to attackers. Sybil attacks are a common issue, especially in decentralized networks and networks that are deployed in scenarios with irregular or unreliable Internet connectivity. The lack of a central authority that can be contacted at any time allows attackers to introduce arbitrary amounts of nodes into the network and manipulate its behavior according to the attacker’s goals, by posing as a majority participant. Depending on the structure of the network, employing Sybil node detection schemes may be difficult, and low powered Internet of Things devices are usually unable to perform impactful amounts of work for proof-of-work based schemes. In this paper, we present Rechained, a scheme that monetarily disincentivizes the creation of Sybil identities for networks that can operate with intermittent or no Internet connectivity. We introduce a new revocation mechanism for identities, tie them into the concepts of self-sovereign identities, and decentralized identifiers. Case-studies are used to discuss upper- and lower-bounds for the costs of Sybil identities and, therefore, the provided security level. Furthermore, we formalize the protocol using Colored Petri Nets to analyze its correctness and suitability. Proof-of-concept implementations are used to evaluate the performance of our scheme on low powered hardware as it might be found in Internet of Things applications.