bluuebunny/crossref_metadata_2025 huggingface dataset:
Marker Development for Differentiation of Fusarium oxysporum f. sp. Niveum Race 3 from Races 1 and 2
Authors: Hudson Owen , Waliullah Sumyya , Fulton James C. , Ji Pingsheng , Dufault Nicholas S. , Keinath Anthony , Ali Md Emran
Published: January 2021

Fusarium wilt of watermelon, caused by Fusarium oxysporum f. sp. niveum (FON), is pathogenic only to watermelon and has become one of the main limiting factors in watermelon production internationally. Detection methods for this pathogen are limited, with few published molecular assays available to differentiate FON from other formae speciales of F. oxysporum. FON has four known races that vary in virulence but are difficult and costly to differentiate using traditional inoculation methods and only race 2 can be differentiated molecularly. In this study, genomic and chromosomal comparisons facilitated the development of a conventional polymerase chain reaction (PCR) assay that could differentiate race 3 from races 1 and 2, and by using two other published PCR markers in unison with the new marker, the three races could be differentiated. The new PCR marker, FNR3-F/FNR3-R, amplified a 511 bp region on the “pathogenicity chromosome” of the FON genome that is absent in race 3. FNR3-F/FNR3-R detected genomic DNA down to 2.0 pg/µL. This marker, along with two previously published FON markers, was successfully applied to test over 160 pathogenic FON isolates from Florida, Georgia, and South Carolina. Together, these three FON primer sets worked well for differentiating races 1, 2, and 3 of FON. For each marker, a greater proportion (60 to 90%) of molecular results agreed with the traditional bioassay method of race differentiation compared to those that did not. The new PCR marker should be useful to differentiate FON races and improve Fusarium wilt research.
Platelet-Rich Plasma Applications for Achilles Tendon Repair: A Bridge between Biology and Surgery
Authors: Padilla Sabino , Sánchez Mikel , Vaquerizo Victor , Malanga Gerard A. , Fiz Nicolás , Azofra Juan , Rogers Christopher J. , Samitier Gonzalo , Sampson Steven , Seijas Roberto , Elorriaga Ricardo , Taunton Jack , Boehm Frank , Prado Roberto , Cugat Ramón , Anitua Eduardo
Published: January 2021

Achilles tendon ruptures are very common tendon ruptures and their incidence is increasing in modern society, resulting in work incapacity and months off sport, which generate a need for accelerated and successful therapeutic repair strategy. Platelet-rich plasma (PRP) is emerging as adjuvant human blood-derived constructs to assist Achilles tendon rupture treatment. However, myriad PRP preparation methods in conjunction with poor standardization in the modalities of their applications impinge on the consistent effectiveness of clinical and structural outcomes regarding their therapeutic efficacy. The purpose of this review is to provide some light on the application of PRP for Achilles tendon ruptures. PRP has many characteristics that make it an attractive treatment. Elements such as the inclusion of leukocytes and erythrocytes within PRP, the absence of activation and activation ex vivo or in vivo, the modality of application, and the adjustment of PRP pH can influence the biology of the applied product and result in misleading therapeutic conclusions. The weakest points in demonstrating their consistent effectiveness are primarily the result of myriad PRP preparation methods and the poor standardization of modalities for their application. Selecting the right biological scaffold and applying it correctly to restitutio ad integrum of ruptured Achilles tendons remains a daunting and complex task.
Spent Brewer’s Yeast as a Source of Insoluble β-Glucans
Authors: Avramia Ionut , Amariei Sonia
Published: January 2021

In the brewing process, the consumption of resources and the amount of waste generated are high and due to a lot of organic compounds in waste-water, the capacity of natural regeneration of the environment is exceeded. Residual yeast, the second by-product of brewing is considered to have an important chemical composition. An approach with nutritional potential refers to the extraction of bioactive compounds from the yeast cell wall, such as β-glucans. Concerning the potential food applications with better textural characteristics, spent brewer’s yeast glucan has high emulsion stability and water-holding capacity fitting best as a fat replacer in different food matrices. Few studies demonstrate the importance and nutritional role of β-glucans from brewer’s yeast, and even less for spent brewer’s yeast, due to additional steps in the extraction process. This review focuses on describing the process of obtaining insoluble β-glucans (particulate) from spent brewer’s yeast and provides an insight into how a by-product from brewing can be converted to potential food applications.
Cytological Observations and Bulked-Segregant Analysis Coupled Global Genome Sequencing Reveal Two Genes Associated with Pollen Fertility in Tetraploid Rice
Authors: Kamara Nabieu , Jiao Yamin , Lu Zijun , Aloryi Kelvin Dodzi , Wu Jinwen , Liu Xiangdong , Shahid Muhammad Qasim
Published: January 2021

Neo-tetraploid rice with high fertility is a useful germplasm for polyploid rice breeding, which was developed from the crossing of different autotetraploid rice lines. However, little information is available on the molecular mechanism underlying the fertility of neo-tetraploid rice. Here, two contrasting populations of tetraploid rice, including one with high fertility (hereafter referred to as JG) and another with low fertility (hereafter referred to as JD), were generated by crossing Huaduo 3 (H3), a high fertility neo-tetraploid rice that was developed by crossing Jackson-4x with 96025-4x, and Huajingxian74-4x (T452), a low fertility autotetraploid rice parent. Cytological, global genome sequencing-based bulked-segregant (BSA-seq) and CRISPR/Cas9 technology were employed to study the genes associated with pollen fertility in neo-tetraploid rice. The embryo sacs of JG and JD lines were normal; however, pollen fertility was low in JD, which led to scarce fertilization and low seed setting. Cytological observations displayed low pollen fertility (25.1%) and approximately 31.3 and 27.2% chromosome lagging at metaphase I and II, and 28.8 and 24.8% chromosome straggling at anaphase I and II in JD, respectively. BSA-seq of F2–3 generations and RNA-seq of F4 generation detected a common fragment, i.e., 18,915,234–19,500,000, at chromosome 7, which was comprised of 78 genes associated with fertility. Among 78 genes, 9 genes had been known to be involved in meiosis and pollen development. Two mutants ny1 (LOC_Os07g32406) and ny2 (LOC_Os07g32040) were generated by CRISPR/Cas9 knockout in neo-tetraploid rice, and which exhibited low pollen fertility and abnormal chromosome behavior. Our study revealed that two unknown genes, LOC_Os07g32406 (NY1) and LOC_Os07g32040 (NY2) play an important role in pollen development of neo-tetraploid rice and provides a new perspective about the genetic mechanisms of fertility in polyploid rice.
Delivery of miR-424-5p via Extracellular Vesicles Promotes the Apoptosis of MDA-MB-231 TNBC Cells in the Tumor Microenvironment
Authors: Zhou Yueyuan , Yamamoto Yusuke , Takeshita Fumitaka , Yamamoto Tomofumi , Xiao Zhongdang , Ochiya Takahiro
Published: January 2021

Programmed cell death ligand-1 (PD-L1) overexpressed on cancer cells has emerged as a key inhibitor that maintains the immunosuppressive microenvironment through its interaction with the PD-1 receptor in cancer. Here, we demonstrated that miR-424-5p delivery via extracellular vesicles (EVs) derived from adipose tissue-mesenchymal stromal cells (AT-MSCs) partly promotes proinflammation and enhances antitumor cytotoxicity in vitro and in vivo. Triple negative breast cancer (TNBC) exhibits increased expression of PD-L1, and PD-L1 is positively correlated with the overall survival of patients with TNBC. PD-L1 shows relatively higher expression in MDA-MB-231 (MM231) cells and can be downregulated by miR-424-5p. Furthermore, miR-424-5p transported by EVs can increase the secretion of proinflammatory cytokines, decrease the secretion of anti-inflammatory cytokines and promote the apoptosis of tumor cells. The intratumoral administration of miR-424-5p-EVs significantly slowed tumor growth. In conclusion, these results demonstrate that EVs may serve as a delivery system for novel immunotherapies for TNBC through the miR-424-5p/PD-L1 pathway.
Substrate Scope for Human Histone Lysine Acetyltransferase KAT8
Authors: Proietti Giordano , Wang Yali , Punzo Chiara , Mecinović Jasmin
Published: January 2021

Biomedically important histone lysine acetyltransferase KAT8 catalyses the acetyl coenzyme A-dependent acetylation of lysine on histone and other proteins. Here, we explore the ability of human KAT8 to catalyse the acetylation of histone H4 peptides possessing lysine and its analogues at position 16 (H4K16). Our synthetic and enzymatic studies on chemically and structurally diverse lysine mimics demonstrate that KAT8 also has a capacity to acetylate selected lysine analogues that possess subtle changes on the side chain and main chain. Overall, this work highlights that KAT8 has a broader substrate scope beyond natural lysine, and contributes to the design of new chemical probes targeting KAT8 and other members of the histone lysine acetyltransferase (KAT) family.
When Does the Cytokine Storm Begin in COVID-19 Patients? A Quick Score to Recognize It
Authors: Cappanera Stefano , Palumbo Michele , Kwan Sherman H. , Priante Giulia , Martella Lucia Assunta , Saraca Lavinia Maria , Sicari Francesco , Vernelli Carlo , Di Giuli Cinzia , Andreani Paolo , Mariottini Alessandro , Francucci Marsilio , Sensi Emanuela , Costantini Monya , Bruzzone Paolo , D’Andrea Vito , Gioia Sara , Cirocchi Roberto , Tiri Beatrice
Published: January 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that is responsible for coronavirus disease 2019 (COVID-19), which has rapidly spread across the world, becoming a pandemic. The “cytokine storm” (CS) in COVID-19 leads to the worst stage of illness, and its timely control through immunomodulators, corticosteroids, and cytokine antagonists may be the key to reducing mortality. After reviewing published studies, we proposed a Cytokine Storm Score (CSs) to identify patients who were in this hyperinflammation state, and at risk of progression and poorer outcomes. We retrospectively analyzed 31 patients admitted to Infectious Disease Department in “St. Maria” Hospital in Terni with confirmed SARS-CoV-2 infections, and analyzed the “CS score” (CSs) and the severity of COVID-19. Then we conducted a prospective study of COVID-19 patients admitted after the definition of the CSscore. This is the first study that proposes and applies a new score to quickly identify COVID-19 patients who are in a hyperinflammation stage, to rapidly treat them in order to reduce the risk of intubation. CSs can accurately identify COVID-19 patients in the early stages of a CS, to conduct timely, safe, and effect administration of immunomodulators, corticosteroids, and cytokine antagonists, to prevent progression and reduce mortality.
Adherence to and the Maintenance of Self-Management Behaviour in Older People with Musculoskeletal Pain—A Scoping Review and Theoretical Models
Authors: Söderlund Anne , von Heideken Wågert Petra
Published: January 2021

(1) Background: Adherence to and the maintenance of treatment regimens are fundamental for pain self-management and sustainable behavioural changes. The first aim was to study older adults’ (>65 years) levels of adherence to and maintenance of musculoskeletal pain self-management programmes in randomized controlled trials. The second aim was to suggest theoretical models of adherence to and maintenance of a behaviour. (2) Methods: The study was conducted in accordance with the recommendations for a scoping review and the PRISMA-ScR checklist. Capability, motivation and opportunity were used to categorize the behavioural change components in the theoretical models. (3) Results: Among the four studies included, components targeting adherence were reported in one study, and maintenance was reported in two studies. Adherence was assessed by the treatment attendance rates, and maintenance was assessed by the follow-up data of outcome measures. For adherence, the capability components were mostly about education and the supervision, grading and mastery of exercise to increase self-efficacy. The motivation components included the readiness to change, self-monitoring and goal setting; and the opportunity components included booster sessions, feedback and social support. For maintenance, the capability components consisted of identifying high-risk situations for relapse and problem-solving skills. The motivation components included self-regulation and self-efficacy for problem solving, and the opportunity components included environmental triggers and problem solving by using social support. (4) Conclusion: There are several behavioural change components that should be used to increase older adults’ levels of adherence to and maintenance of a pain self-management behaviour.
Impact of Different Positive End-Expiratory Pressures on Lung Mechanics in the Setting of Moderately Elevated Intra-Abdominal Pressure and Acute Lung Injury in a Porcine Model
Authors: Fiedler Mascha O. , Simeliunas Emilis , Deutsch B. Luise , Diktanaite Dovile , Harms Alexander , Brune Maik , Dietrich Maximilian , Uhle Florian , Weigand Markus A. , Kalenka Armin
Published: January 2021

The effects of a moderately elevated intra-abdominal pressure (IAP) on lung mechanics in acute respiratory distress syndrome (ARDS) have still not been fully analyzed. Moreover, the optimal positive end-expiratory pressure (PEEP) in elevated IAP and ARDS is unclear. In this paper, 18 pigs under general anesthesia received a double hit lung injury. After saline lung lavage and 2 h of injurious mechanical ventilation to induce an acute lung injury (ALI), an intra-abdominal balloon was filled until an IAP of 10 mmHg was generated. Animals were randomly assigned to one of three groups (group A = PEEP 5, B = PEEP 10 and C = PEEP 15 cmH2O) and ventilated for 6 h. We measured end-expiratory lung volume (EELV) per kg bodyweight, driving pressure (ΔP), transpulmonary pressure (ΔPL), static lung compliance (Cstat), oxygenation (P/F ratio) and cardiac index (CI). In group A, we found increases in ΔP (22 ± 1 vs. 28 ± 2 cmH2O; p = 0.006) and ΔPL (16 ± 1 vs. 22 ± 2 cmH2O; p = 0.007), with no change in EELV/kg (15 ± 1 vs. 14 ± 1 mL/kg) when comparing hours 0 and 6. In group B, there was no change in ΔP (26 ± 2 vs. 25 ± 2 cmH2O), ΔPL (19 ± 2 vs. 18 ± 2 cmH2O), Cstat (21 ± 3 vs. 21 ± 2 cmH2O/mL) or EELV/kg (12 ± 2 vs. 13 ± 3 mL/kg). ΔP and ΔPL were significantly lower after 6 h when comparing between group C and A (21 ± 1 vs. 28 ± 2 cmH2O; p = 0.020) and (14 ± 1 vs. 22 ± 2 cmH2O; p = 0.013)). The EELV/kg increased over time in group C (13 ± 1 vs. 19 ± 2 mL/kg; p = 0.034). The P/F ratio increased in all groups over time. CI decreased in groups B and C. The global lung injury score did not significantly differ between groups (A: 0.25 ± 0.05, B: 0.21 ± 0.02, C: 0.22 ± 0.03). In this model of ALI, elevated IAP, ΔP and ΔPL increased further over time in the group with a PEEP of 5 cmH2O applied over 6 h. This was not the case in the groups with a PEEP of 10 and 15 cmH2O. Although ΔP and ΔPL were significantly lower after 6 hours in group C compared to group A, we could not show significant differences in histological lung injury score.
Real Time PCR and Culture-Based Virus Isolation Test in Clinically Recovered Patients: Is the Subject Still Infectious for SARS-CoV2?
Authors: Manzulli Viviana , Scioscia Giulia , Giganti Giulio , Capobianchi Maria Rosaria , Lacedonia Donato , Pace Lorenzo , Cipolletta Dora , Tondo Pasquale , De Nittis Rosella , Rondinone Valeria , Serrecchia Luigina , Parisi Antonio , Galante Domenico , Lo Caputo Sergio , Santantonio Teresa Antonia , Moschetta Damiana , Dattoli Vitangelo , Fasanella Antonio , Foschino Barbaro Maria Pia
Published: January 2021

Background. The highly variable manifestation of the COVID-19 disease, from completely asymptomatic to fatal, is both a clinical and a public health issue. The criteria for discharge of hospitalized patients have been based so far on the negative result of Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) tests, but the persistence of viral fragments may exceed that of the integral virus by weeks. The aim of our study was to verify the clearance of the virus at viral culture in patients hospitalized for COVID-19 that have clinically recovered but are still positive on nasopharyngeal swab. Methods. The study was conducted in hospitalized patients with positive RT-PCR on nasopharyngeal swab. Patients included were from asymptomatic to severe cases and performed nasopharyngeal control swabbing on day 14 for asymptomatic patient or at least three days after remission of symptoms. RT-PCR positive specimens were sent to a biosafety level 3 laboratory for viral culture. Results. We performed a combined analysis of RT-PCR and a highly sensitive in vitro culture from 84 samples of hospitalized patients. The average age was 46 ± 20.29, and 40.5% of the subjects had radiologically confirmed pneumonia, with average PaO2 of 72.35 ± 12.12and P/F ratio of 315 ± 83.15. Ct values for the N gene were lower in the first swab than in the control one (p < 0.001). The samples from 83 patients were negative at viral culture, and RT-PCR on the respective supernatants always confirmed the absence of viral growth. Conclusions. Our preliminary results demonstrate that patients clinically recovered for at least three days show the viral clearance at viral culture, and presumably they continued to not be contagious.
A Morphable Physically Unclonable Function and True Random Number Generator Using a Commercial Magnetic Memory
Authors: Khan Mohammad Nasim Imtiaz , Cheng Chak Yuen , Lin Sung Hao , Ash-Saki Abdullah , Ghosh Swaroop
Published: January 2021

We use commercial magnetic memory to realize morphable security primitives, a Physically Unclonable Function (PUF) and a True Random Number Generator (TRNG). The PUF realized by manipulating the write time and the TRNG is realized by tweaking the number of write pulses. Our analysis indicates that more than 75% bits in the PUF are unusable without any correction due to their inability to exhibit any randomness. We exploit temporal randomness of working columns to fix the unusable columns and write latency to fix the unusable rows during the enrollment. The intra-HD, inter-HD, energy, bandwidth and area of the proposed PUF are found to be 0, 46.25%, 0.14 pJ/bit, 0.34 Gbit/s and 0.385 μm2/bit (including peripherals) respectively. The proposed TRNG provides all possible outcomes with a standard deviation of 0.0062, correlation coefficient of 0.05 and an entropy of 0.95. The energy, bandwidth and area of the proposed TRNG is found to be 0.41 pJ/bit, 0.12 Gbit/s and 0.769 μm2/bit (including peripherals). The performance of the proposed TRNG has also been tested with NIST test suite. The proposed designs are compared with other magnetic PUFs and TRNGs from other literature.
Grey Systems Theory as an Effective Method for Analyzing Scarce, Incomplete and Uncertain Data on the Example of a Survey of Public Perceptions of Safety in Urban Spaces
Authors: Gerus-Gościewska Małgorzata , Gościewski Dariusz
Published: January 2021

Many processes and phenomena that occur in the natural and social environment have a complex character, and the interdependencies between social and economic phenomena are most often analyzed by identifying the relationships between multiple factors that shape urban space. Decisions concerning the visual attributes of cities are usually made by urban planners and civil officers, whereas social preferences are rarely considered in the planning process. The latest research indicates that urban planners should account for the needs and expectations of local residents who are the users of public spaces in cities. This paper discusses the results of selected research studies investigating the impact of geospatial attributes on perceptions of safety in urban areas. The theories that are used to improve safety in cities and selected methods for analyzing spatial data were presented. The analyzed attributes were selected by brainstorming, a heuristic technique for solving research problems. The selected attributes were ranked in a survey performed on an accidental (convenience) sample. In this study, Grey Relational Analysis (GRA), a type of Grey Systems Theory (GST) which supports the use of incomplete, uncertain and scarce data, was applied. The advantages of grey systems over statistical methods in analyses of spatial data were presented. Grey system analyses generate sequences of significant geospatial attributes and indicate which factors exert the greatest influence on the examined phenomenon. The results can be used to solve practical problems related to the shaping of space.
Polymorphisms of ATP-Binding Cassette, Sub-Family A, Member 4 (rs560426 and rs481931) and Non-Syndromic Cleft Lip/Palate: A Meta-Analysis
Authors: Imani Mohammad Moslem , Sadeghi Masoud , Tadakamadla Santosh Kumar , Brühl Annette , Sadeghi Bahmani Dena , Taheri Mohammad , Brand Serge
Published: January 2021

Background: A number of genes are associated with the incidence of non-syndromic cleft lip/palate (NSCL/P). Studies have shown a significant association between polymorphisms of ATP-binding cassette, sub-family A, member 4 (ABCA4) with the risk of NSCL/P. The present meta-analysis assessed the association between ABCA4 polymorphisms (rs560426 and rs481931) and the NSCL/P risk by reviewing case-control studies. Methods: Four databases (Scopus; Cochrane Library; Web of Science; and PubMed) were searched for articles published up to June 2020. The Review Manager 5.3 software was used to calculate the crude odds ratio (OR) and 95% confidence interval (CI). Both subgroup analyses for ethnicity and source of controls and a meta-regression related to publication year were conducted. Results: Of 94 retrieved studies, 12 were analyzed in this meta-analysis (2859 NSCL/P patients and 3792 controls for ABCA4 rs560426 polymorphism and 1333 NSCL/P patients and 1884 controls for ABCA4 rs481931 polymorphism). Overall, there was no significant association between both polymorphisms and the risk of NSCL/P. However, subgroup analysis demonstrated that there was a higher risk of NSCL/P for specific models: the allelic model (OR = 1.13; p = 0.03), the homozygote model (OR = 1.53; p = 0.04), and the recessive model (OR = 1.30; p = 0.03) in the Asian ethnicity for the rs560426 polymorphism. Conclusion: The findings confirmed that the NSCL/P risk was significantly associated with the G allele and GG genotype of rs560426 polymorphism but not for rs481931 polymorphism. There were no associations between both polymorphisms (rs560426 and rs481931) and the NSCL/P risk in those of European descent and the mixed ethnicities.
Insights into the Geomicrobiology of Biovermiculations from Rock Billet Incubation Experiments
Authors: Kelly Hilary , Spilde Michael N. , Jones Daniel S. , Boston Penelope J.
Published: January 2021

Biovermiculations are uniquely patterned organic rich sediment formations found on the walls of caves and other subterranean environments. These distinctive worm-like features are the combined result of physical and biological processes. The diverse microbial communities that inhabit biovermiculations may corrode the host rock, form secondary minerals, and produce biofilms that stabilize the sediment matrix, thus altering cave surfaces and contributing to the formation of these wall deposits. In this study, we incubated basalt, limestone, and monzonite rock billets in biovermiculation mixed natural community enrichments for 468–604 days, and used scanning electron microscopy (SEM) to assess surface textures and biofilms that developed over the course of the experiment. We observed alteration of rock billet surfaces associated with biofilms and microbial filaments, particularly etch pits and other corrosion features in olivine and other silicates, calcite dissolution textures, and the formation of secondary minerals including phosphates, clays, and iron oxides. We identified twelve distinct biofilm morphotypes that varied based on rock type and the drying method used in sample preparation. These corrosion features and microbial structures inform potential biological mechanisms for the alteration of cave walls, and provide insight into possible small-scale macroscopically visible biosignatures that could augment the utility of biovermiculations and similarly patterned deposits for astrobiology and life detection applications.
The Remarkable Metabolism of Vickermania ingenoplastis: Genomic Predictions
Authors: Opperdoes Fred R. , Butenko Anzhelika , Zakharova Alexandra , Gerasimov Evgeny S. , Zimmer Sara L. , Lukeš Julius , Yurchenko Vyacheslav
Published: January 2021

A recently redescribed two-flagellar trypanosomatid Vickermania ingenoplastis is insensitive to the classical inhibitors of respiration and thrives under anaerobic conditions. Using genomic and transcriptomic data, we analyzed its genes of the core metabolism and documented that subunits of the mitochondrial respiratory complexes III and IV are ablated, while those of complexes I, II, and V are all present, along with an alternative oxidase. This explains the previously reported conversion of glucose to acetate and succinate by aerobic fermentation. Glycolytic pyruvate is metabolized to acetate and ethanol by pyruvate dismutation, whereby a unique type of alcohol dehydrogenase (shared only with Phytomonas spp.) processes an excess of reducing equivalents formed under anaerobic conditions, leading to the formation of ethanol. Succinate (formed to maintain the glycosomal redox balance) is converted to propionate by a cyclic process involving three enzymes of the mitochondrial methyl-malonyl-CoA pathway, via a cyclic process, which results in the formation of additional ATP. The unusual structure of the V. ingenoplastis genome and its similarity with that of Phytomonas spp. imply their relatedness or convergent evolution. Nevertheless, a critical difference between these two trypanosomatids is that the former has significantly increased its genome size by gene duplications, while the latter streamlined its genome.
An Integrated Approach to Identify New Anti-Filarial Leads to Treat River Blindness, a Neglected Tropical Disease
Authors: Tyagi Rahul , Bulman Christina A. , Cho-Ngwa Fidelis , Fischer Chelsea , Marcellino Chris , Arkin Michelle R. , McKerrow James H. , McNamara Case W. , Mahoney Matthew , Tricoche Nancy , Jawahar Shabnam , Janetka James W. , Lustigman Sara , Sakanari Judy , Mitreva Makedonka
Published: January 2021

Filarial worms cause multiple debilitating diseases in millions of people worldwide, including river blindness. Currently available drugs reduce transmission by killing larvae (microfilariae), but there are no effective cures targeting the adult parasites (macrofilaricides) which survive and reproduce in the host for very long periods. To identify effective macrofilaricides, we carried out phenotypic screening of a library of 2121 approved drugs for clinical use against adult Brugia pahangi and prioritized the hits for further studies by integrating those results with a computational prioritization of drugs and associated targets. This resulted in the identification of 18 hits with anti-macrofilaricidal activity, of which two classes, azoles and aspartic protease inhibitors, were further expanded upon. Follow up screening against Onchocerca spp. (adult Onchocerca ochengi and pre-adult O. volvulus) confirmed activity for 13 drugs (the majority having IC50 < 10 μM), and a counter screen of a subset against L. loa microfilariae showed the potential to identify selective drugs that prevent adverse events when co-infected individuals are treated. Stage specific activity was also observed. Many of these drugs are amenable to structural optimization, and also have known canonical targets, making them promising candidates for further optimization that can lead to identifying and characterizing novel anti-macrofilarial drugs.
Effect of Crude Oil on Growth, Oxidative Stress and Response of Antioxidative System of Two Rye (Secale cereale L.) Varieties
Authors: Skrypnik Liubov , Maslennikov Pavel , Novikova Anastasia , Kozhikin Mikhail
Published: January 2021

Rye (Secale cereale L.) is one of the most important cereal crops in Eastern and Northern Europe, showing better tolerance to environmental stress factors compared to wheat and triticale. Plant response to the crude oil-polluted soil depends on plant species, oil concentration, time of exposure, etc. The current study is aimed at investigating the growth, oxidative stress and the response of antioxidative system of two rye varieties (Krona and Valdai) cultivated on crude oil-contaminated soils at different concentrations (1.5, 3.0, 6.0, and 12.0%). Inhibition of rye growth was observed at crude oil concentrations of above 3% for above-ground plant parts and of above 1.5% for roots. A decrease in content of chlorophyll a and total chlorophylls in Krona variety was detected at 1.5% oil concentration in soil and in Valdai variety at 3% oil concentration. Compared with the control, the content of malondialdehyde was significantly increased in the Krona variety at 3% oil concentration and in Valdai variety at 6% oil concentration. The crude oil-induced oxidative stress was minimized in rye plants by the enhanced contents of low-molecular antioxidants (proline, non-protein thiols, ascorbic acid, phenolic compounds) and activities of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione peroxidase. The strongest positive correlation was detected between the content of malondialdehyde and contents of proline (r = 0.89–0.95, p ≤ 0.05) and phenolic compounds (r = 0.90–0.94, p ≤ 0.05) as well as superoxide dismutase activity (r = 0.81–0.90, p ≤ 0.05). Based on the results of a comprehensive analysis of growth and biochemical parameters and of the cluster analysis, Valdai variety proved to be more resistant to oil pollution. Due to this, Valdai variety is considered to be a promising rye variety for cultivation on moderately oil-polluted soils in order to decontaminate them. At the same time, it is necessary to conduct further studies aimed at investigating oil transformation processes in the soil-rye system, which would make it possible to determine the efficiency of using this cereal for soil remediation.
Robot Tutoring of Multiplication: Over One-Third Learning Gain for Most, Learning Loss for Some
Authors: Hoorn Johan F. , Huang Ivy S. , Konijn Elly A. , van Buuren Lars
Published: January 2021

In the design of educational robots, it appears to be undecided as to whether robots should show social behaviors and look human-like or whether such cues are insignificant for learning. We conducted an experiment with different designs of social robots built from the same materials, which is unique in robotics research. The robots rehearsed multiplication tables with primary school children in Hong Kong, which is a user group not easily or often accessed. The results show that affective bonding tendencies may occur but did not significantly contribute to the learning progress of these children, which was perhaps due to the short interaction period. Nonetheless, 5 min of robot tutoring improved their scores by about 30%, while performance dropped only for a few challenged children. We discuss topics, such as teaching language skills, which may be fostered by human likeness in appearance and behaviors; however, for Science, Technology, Engineering, and Mathematics (STEM)-related subjects, the social aspects of robots hardly seem to matter.
Smart Sensors for Healthcare and Medical Applications
Authors: Formica Domenico , Schena Emiliano
Published: January 2021

This special issue on “Smart Sensors for Healthcare and Medical Applications” focuses on new sensing technologies, measurement techniques, and their applications in medicine and healthcare [...]
Wavelength Dependent Graphene Oxide-Based Optical Microfiber Sensor for Ammonia Gas
Authors: Girei Saad Hayatu , Alkhabet Mohammed Majeed , Kamil Yasmin Mustapha , Lim Hong Ngee , Mahdi Mohd Adzir , Yaacob Mohd Hanif
Published: January 2021

Ammonia detection in ambient air is critical, given its implication on the environment and human health. In this work, an optical fiber tapered to a 20 µm diameter and coated with graphene oxide was developed for absorbance response monitoring of ammonia at visible (500–700 nm) and near-infrared wavelength regions (700–900 nm). The morphology, surface characteristics, and chemical composition of the graphene oxide samples were confirmed by a field emission scanning electron microscope, an atomic force microscope, X-ray diffraction, and an energy dispersion X-ray. The sensing performance of the graphene oxide-coated optical microfiber sensor towards ammonia at room temperature revealed better absorbance response at the near-infrared wavelength region compared to the visible region. The sensitivity, response and recovery times at the near-infrared wavelength region were 61.78 AU/%, 385 s, and 288 s, respectively. The sensitivity, response and recovery times at the visible wavelength region were 26.99 AU/%, 497 s, and 192 s, respectively. The selectivity of the sensor towards ammonia was affirmed with no response towards other gases.
Efficacy of Feed-Based Formalin-Killed Vaccine of Streptococcus iniae Stimulates the Gut-Associated Lymphoid Tissues and Immune Response of Red Hybrid Tilapia
Authors: Hayat Mohammad , Mohd Yusoff Md Sabri , Samad Mohd Jamil , Abdul Razak Intan Shameha , Md Yasin Ina Salwany , Thompson Kim D. , Hasni Khalil
Published: January 2021

Red hybrid tilapia were fed a formalin-killed oral Streptococcus iniae vaccine (FKV) in the present study was assessed. Three hundred Red hybrid tilapia 80 ± 10 g were divided into five groups (1A, 1B, 2A, 2B, and Cx), each consisting of 60 fish. Fish from Groups 1A, 1B, 2A, and 2B were fed with FKV over different periods of administration, while Group 2B was the only group of fish to receive an oral booster vaccination on day 14- and 21-days post-vaccination (dpv). Group Cx was fed with normal pellets containing no vaccine as a control group. At four weeks post-vaccination (wpv), all fish were experimentally infected with S. iniae. Groups 2A and 2B had the lowest level of mortalities following vaccination (45% and 30%, respectively) compared to Groups 1A and 1B (80% and 55%, respectively), while the level of mortalities in Group Cx was 100%. All vaccinated groups showed a significant increase in anti-S. iniae IgM levels (p < 0.05) in serum, mucus, and gut-lavage, while Group Cx did not (p > 0.05) and all fish in this group died by five weeks post-infection. In conclusion, fish fed with the S. iniae FKV had a greater level of protection against S. iniae, with increased specific antibody response to the vaccine and there was also evidence of GALT stimulation by the vaccine.
Linear B-Cell Epitopes in Human Norovirus GII.4 Capsid Protein Elicit Blockade Antibodies
Authors: Moeini Hassan , Afridi Suliman Qadir , Donakonda Sainitin , Knolle Percy A. , Protzer Ulrike , Hoffmann Dieter
Published: January 2021

Human norovirus (HuNoV) is the leading cause of nonbacterial gastroenteritis worldwide with the GII.4 genotype accounting for over 80% of infections. The major capsid protein of GII.4 variants is evolving rapidly, resulting in new epidemic variants with altered antigenic potentials that must be considered for the development of an effective vaccine. In this study, we identify and characterize linear blockade B-cell epitopes in HuNoV GII.4. Five unique linear B-cell epitopes, namely P2A, P2B, P2C, P2D, and P2E, were predicted on the surface-exposed regions of the capsid protein. Evolving of the surface-exposed epitopes over time was found to correlate with the emergence of new GII.4 outbreak variants. Molecular dynamic simulation (MD) analysis and molecular docking revealed that amino acid substitutions in the putative epitopes P2B, P2C, and P2D could be associated with immune escape and the appearance of new GII.4 variants by affecting solvent accessibility and flexibility of the antigenic sites and histo-blood group antigens (HBAG) binding. Testing the synthetic peptides in wild-type mice, epitopes P2B (336–355), P2C (367–384), and P2D (390–400) were recognized as GII.4-specific linear blockade epitopes with the blocking rate of 68, 55 and 28%, respectively. Blocking rate was found to increase to 80% using the pooled serum of epitopes P2B and P2C. These data provide a strategy for expanding the broad blockade potential of vaccines for prevention of NoV infection.
FEATURES OF CALCULATION OF CENTRIFUGED AND VIBRO-CENTRIFUGED REINFORCED CONCRETE STRUCTURES BY DIFFERENTIAL CONSTRUCTION CHARACTERISTICS OF CONCRETE
Authors: Mailyan L. , Stel'makh S. , Shcherban' E. , Dotsenko N.
Published: January 2021

This article raises one of the most popular questions: evaluation of calculations of differential design parameters of centrifuged and vibro-centrifuged concrete. It is established that various layers of concrete of annular cross-section in the process of centrifugation and vibro-centrifugation are influenced by forces that differ from each other in the magnitude of the impact. This leads to completely different indicators of concrete layers in density, strength, deformability and modulus of elasticity. Centrifugal and centripetal forces are the main factors that are worth to pay attention to and to use in the calculated dependencies as arguments. Therefore, in general calculations, which take into account the change in the structural parameters of concrete, it is advisable to use the structural indicators or their increase as functions, and as independent variables – the forces of inertia of rotation, which, in turn, are the dependence of the distance from the center of rotation and the angular velocity of rotation. As a result, the calculated dependencies are derived for the differentiated accounting of changes in all the characteristics of concrete necessary for calculating, which gives grounds to enter into the calculation of structures the strength and deformation indicators of concrete that differ in cross section, and to use the existing reserves of the bearing capacity of elements.
Statistical Assessment of the Changing Climate of Vadodara City, India During 1969-2006
Authors: Kandya Anurag , Sarkar Jayanta , Chhabra Abha , Chauhan Shreya , Khatri Dishant , Vaghela Aditya , Kolte Santosh
Published: January 2021

There is a global change in the climate and cities are looked as the key culprits of this change. Indiawill undergo an immense urbanization in the coming decades, doubling its urbanpopulation by 2050. Thus it is very important to understand the dynamics of the changing urban climate of Indian cities. With this background, the present urban climate change study is conducted for the Vadodara city located in the state of Gujarat, Indiausing the hourly Dry Bulb Temperature (DBT) and Wet Bulb Temperature (WBT) available at 00-hr, 03-hr, 06-hr, 09-hr, 12-hr, 15-hr, 18-hr and 21-hr for a period of 37 years (1969-2006). The hourly DBT and WBT for the 12 months were bunched on hourly-monthlybasis (thereby having 192 specific bunches) for the study period which were then analyzed using the Mann-Kendall trend test at different confidence limits (90%, 95% and 99%). The findings reveal that there is an overall increase in the temperature of the city as for 78% of the time the DBT had an increasing trend and 52% of the time WBT had an increasing trend. For the first half of the day (03-hr to 12-hr) both the DBT and WBT had an overall increasing trend while in the second half of the day (15-hr to 0-hr) DBT had an overall increasing trend and WBT had an overall decreasing trend. This contrasting behavior of the DBT and WBT was specifically seen in the months of May-July which are largely the monsoon months. For both DBT and WBT, the month of Februaryhad the overall maximum increasing trend while the month of August had the overall maximum decreasing trend. The present study statistically quantifies the changing urban climate of Vadodara city and these findings would not only add to the pool of knowledge to understand the dynamics of the changing urban climate but will be of ready reference for the policy makers to initiate appropriate measures for mitigation and adaptation.
Dainas kā Lejaskurzemes izlokšņu fonētikas un morfoloģijas iezīmju avots
Authors: Markus-Narvila Liene
Published: January 2021

Keywords: folksongs, subdialects, Lejaskurzeme, phonetics, morphology

It has been a long time since linguists have spoken about the levelling of subdialects in Latvia, for instance, linguist Jānis Endzelīns already in the middle of the 20th century, when he spoke about the language of the district of Vidzeme said these significant words: “As elsewhere also in Latvia the standard language exterminates subdialects and partly has already exterminated them. There are many areas where it is hard to find a person who speaks only in subdialect.” Therefore, it is important to identify the most important sources of the acquisition of subdialects, which would be useful to all who are interested in subdialectic studies. One of these sources is Latvian folksongs, which vividly preserve the most prominent phonetic and morphological features. The phonetical and morphological features of the subdialects of Lejaskurzeme have been identified in both folksong materials and in later linguistic sources—in the descriptions of Anna Ābele “Par Rucavas izloksni” (On the Rucava Sub-Dialect (1927)), “Rucavas izloksne” (The Rucava Sub-Dialect (1928)), “Gramzdas draudzes izloksne” (The Sub-Dialect of Gramzda Parish (1929)) and also in the description of Emma Valtere “Pērkones izloksne” (The Pērkone Sub-Dialect (1938)) as well as in late 20th century and early 21st century linguistic sources, which allow a comparison between characteristic subdialectical peculiarities and to track their changes over the years.
This article analyses the most prominent peculiarities of the subdialects of Lejaskurzeme both in folksongs and in subdialectal sources: in phonetics and morphology. The analysis of the folksongs shows that the treasures of our language are still living; moreover, especially the sources of folklore, including folksongs, are still an actual source of studying subdialects, which, if we use correctly, can reveal grammatical, phonetical, and morphological peculiarities.
Latviešu komponistu adaptācijas varianti pēckara Eiropā
Authors: Klotiņš Arnolds
Published: January 2021

Keywords: classical music, style, national romanticism, transformation of creative works, modernism, avant-garde

This article discusses the stylistic and aesthetic transformation of the work of the most prominent Latvian composers, who, as refugees of the Second World War, arrived in Western Europe in 1944 and encountered a different, innovative musical environment there. For those whose creative work in Latvia had been focused on traditional national romanticism, the encounter with musical expressionism and the avant-garde caused a certain shock. The stark differences in style were not only a matter of compositional technique; they also revealed the contradiction between a positivistic worldview and a more adequate musical reflection of the common man during the era.
Longīns Apkalns learned from the ideas and style of expressionism most radically, but Alberts Jērums was much more moderate in this respect—he had already approached expressionism during his studies at the Latvian Conservatory. In his studies at the Paris Conservatory (1945-1950), Tālivaldis Ķeniņš studied the traditions of French neoclassicism and constructivism. Volfgangs Dārziņš adapted neoclassical trends in combination with Béla Bartók’s interpretation of folklore. Jānis Mediņš radicalized his traditional language of music, but did not abandon the paradigm of the music of romanticism. Similarly, Jānis Kalniņš’ music, even in the pre-war period, was not unfamiliar with the border between romanticism and expressionism. The composers who, with their creative work, chose to serve only Latvian society in exile continued in the romantic style.
Folklora un ideoloģija: folkloras teksti latviešu mācību literatūrā PSRS 20. gadsimta 20.–30. gados
Authors: Ļaksa-Timinska Ilze
Published: January 2021

Keywords: diaspora, Marxist-Leninist ideology, propaganda, education in the USSR, Soviet folklore

The focus of this article is Latvian textbooks published in the USSR from 1920-1930 for Latvian pupils living in the Soviet Union, and the use of folklore texts in them. Attention is focused on the principles of the selection of specific texts and how the authors of textbooks interpret folklore texts. This research aims to determine how folklore units available in Soviet Latvian textbooks resonated with the dominant dogmas of the Marxist-Leninist ideology in the USSR.
The first half of the article describes the most important aspects of the Soviet Latvian diaspora and the organization of education. In this part of the study, all Soviet Latvian textbooks issued from 1920-1930 were examined: ABC, Latvian language and literature textbooks, school reading books and chrestomathies, as well as published selections of folklore units (24 books in all), not all of them included folklore texts. The focus of the analysis is only those books in which folklore had been published.
The analyzed folklore units show how these texts can be used for propaganda purposes using various methods: by selectively picking out only those folklore units that correspond to the Marxist-Leninist ideology, commenting and creating paratexts, ignoring Latvian folklore genres (mythical songs, magic tales), using metrics and formulas of classical folklore to create texts with new content glorifying Soviet reality.
Stāstījuma jēdziens un tā adepti latviešu literatūrzinātnē
Authors: Ozoliņš Jānis
Published: January 2021

Keywords: stāstījums, vēstījums, Broņislavs Tabūns, Harijs Hiršs, narratology, Latvian literary scholarship

This article is dedicated to the concept of narrative, exploring the origins of the concept, its character, and use in Latvian literary history and nowadays. The concept of narrative has telltale signs of an epistemological crisis: the meaning of concepts (including narrative) is regularly questioned and expanded. Independent of whether we view a narrative, at the level of microanalysis, as a chain of events (actions) linked by causality, or at the level of macroanalysis, as an ideological concept or an ingredient of cognitive processes, narrative is linguistic in origins and still retains that function. The sources of narratology in Latvian literary scholarship are found in the publications on the theory of fiction by Harijs Hiršs and Broņislavs Tabūns as early as the 1980s. The views of both theoreticians tend to impact the use of the words “vēstījums” (message) and “stāstījums” (narrative) in Latvian: Tabūns used “vēstījums”, whereas Hiršs has used both “vēstījums” and “stāstījums”, originally as synonyms, and later introducing a hierarchical relationship between the two. The different approaches have lead to confusion among literary scholars—to this day, both concepts are sometimes used as synonyms in scholarly literature. The etymology of words and context of their use indicate that ‘stāstījums’ is a more precise translation of the concept of “narrative” than “vēstījums”, for it satisfies the requirements of both classical and postclassical narratology.