bluuebunny/crossref_metadata_2025 huggingface dataset:
Low intensity psychological interventions for the treatment of feeding and eating disorders: a systematic review and meta-analysis
Authors: Davey Emily , Bennett Sophie D. , Bryant-Waugh Rachel , Micali Nadia , Takeda Andrea , Alexandrou Alexia , Shafran Roz
Published: April 2023

Background

Feeding and eating disorders are associated with significant illness burden and costs, yet access to evidence-based care is limited. Low intensity psychological interventions have the potential to increase such access.

Methods

A systematic review and meta-analysis were conducted on the use of low intensity psychological interventions for the treatment of feeding and eating disorders. Studies comparing low intensity psychological interventions against high intensity therapies and non-eating disorder specific psychological interventions were included, as well as those with waiting list control arms. There were three primary outcomes: eating disorder psychopathology, diagnostic and statistical manual of mental disorders (DSM) severity specifier-related outcomes and rates of remission/recovery.

Results

Thirty-three studies met the inclusion criteria, comprising 3665 participants, and 30 studies were included in the meta-analysis. Compared to high intensity therapies, low intensity psychological interventions were equivalent on reducing eating disorder psychopathology (g = − 0.13), more effective at improving DSM severity specifier-related outcomes (g = − 0.15), but less likely to achieve remission/recovery (risk ratio (RR) = 0.70). Low intensity psychological interventions were superior to non-eating disorder specific psychological interventions and waiting list controls across all three primary outcomes.

Conclusion

Overall, findings suggest that low intensity psychological interventions can successfully treat eating disorder symptoms. Few potential moderators had a statistically significant effect on outcome. The number of studies for many comparisons was low and the methodological quality of the studies was poor, therefore results should be interpreted with caution. More research is needed to establish the effectiveness of low intensity psychological interventions for children and young people, as well as for individuals with anorexia nervosa, avoidant/restrictive food intake disorder, pica and rumination disorder.
Eating disorders and oral health: a scoping review
Authors: Presskreischer Rachel , Prado Michael A. , Kuraner S. Emre , Arusilor Isabelle-Maria , Pike Kathleen
Published: April 2023

Background

Screening and treatment guidance for somatic sequalae of eating disorders typically include specifics such as laboratory testing, observable physical signs, and treatment interventions. Oral health guidance is notably sparse or absent from many guidelines. Often, the only mention of oral health is the potential erosion caused by self-induced vomiting and suggests a referral to an oral health professional. The guidelines generally do not include information about education and training of oral health professionals.

Objective

The objective of this research was to explore the literature on eating disorders and oral health including the effects of eating disordered behaviors on oral health and training of oral health professionals to increase their capacity to recognize and appropriately address clinical care needs of individuals with eating disorders.

Methods

A comprehensive scoping review was conducted to investigate what is known about the relationship between eating disorders and oral health and training provided to oral health professionals in recognition and treatment of individuals with eating disorders. The search was completed using PubMed, Embase, Science Direct, Google Scholar, and the Journal of the American Dental Association.

Results

Of 178 articles returned in the initial search, 72 full texts were read, and 44 were included based on eligibility criteria. The retained articles were categorized thematically into articles related to (1) oral health professional education and training, (2) the oral health effects of eating disorders, and (3) patient experiences of oral health care.

Conclusion

Most of the research on the relationship between eating disorders and oral health examines the impact of eating disordered behaviors. There is a significantly smaller literature on the knowledge and training of oral health professionals related to eating disorders and individuals with eating disorders’ experiences of oral health care. Research on education and training of oral health professionals should be expanded globally, taking into consideration the suitability of interventions for diverse models of oral health education and service delivery. Further, there is an opportunity for eating disorder professionals and professional organizations to improve understanding and care of eating disorders by building relationships with oral health providers and professional organizations in their local communities.
Contexts shaping misdemeanor system interventions among people with mental illnesses: qualitative findings from a multi-site system mapping exercise
Authors: Wood Jennifer D. , Watson Amy C. , Pope Leah , Warnock Amanda , Nelson Veronica , Gesser Nili , Zern Adria , Stagoff-Belfort Aaron , de Bibiana Jason Tan , Compton Michael T.
Published: April 2023

Background

People with mental illnesses are disproportionately entangled in the criminal legal system. Historically, this involvement has resulted from minor offending, often accompanied by misdemeanor charges. In recent years, policymakers have worked to reduce the footprint of the criminal legal system. This paper seeks to better understand how misdemeanor systems intervene in the lives of people with mental illnesses.

Methods

System mapping exercises were conducted with misdemeanor system stakeholders from the jurisdictions of Atlanta, Chicago, Manhattan, and Philadelphia. Narrative detail on decision-making and case processing, both generally and in relation to specific types of behavior, including trespassing, retail theft/shoplifting, and simple assault, were coded and analyzed for thematic patterns. Based on the qualitative analysis, this paper offers a conceptual diagram of contexts shaping misdemeanor system interventions among people with mental illnesses.

Results

All four sites have been engaged in efforts to reduce the use of misdemeanor charges both generally and in relation to people with mental illnesses. Decision-makers across all sites experience contexts that shape how, when, and where they intervene, which are: (1) law and policy environments; (2) location of the behavior; (3) expectations of stakeholders; (4) knowledge of mental illnesses; and (5) access to community resources. Law and policy environments expand or constrain opportunities for diversion. The location of offending is relevant to who has a stake in the behavior, and what demands they have. Clinical, experiential, and system-level knowledge of mental illnesses inform a chain of decisions about what to do. The capacity to address mental health needs is contingent on access to social services, including housing.

Conclusion

People making decisions along the criminal legal continuum are critical to illuminating the dynamic, inter-related contexts that facilitate and frustrate attempts to address defendants’ mental health needs while balancing considerations of public safety. Multi-sector, scenario-based or case study exercises could help identify concrete ways of improving each of the contexts that surround whole-of-system decisions.
Empirical validation study and psychometric evaluation of the properties of the populist attitudes scale for the portuguese population
Authors: Falcão Filipe , Jalali Carlos , Costa Patrício
Published: April 2023

Background and objective

Recent developments in Europe and Portugal provide a fertile ground for the rise of populism. Despite the growing interest in the topic, there is no reliable tool to gauge Portuguese citizens’ populist attitudes to date. The Populist Attitudes Scale (POP-AS), developed by Akkerman et al. [1], is one of the best-known instruments for measuring populist attitudes. However, no version for use in the Portuguese population is available. This paper describes the psychometric validation of the POP-AS for the Portuguese population.

Methods

Trustworthy measures of validity suggested by Boateng et al. [2] to address the psychometric features of the POP-AS were approached. A robust psychometrical pipeline evaluated the reliability, construct validity, cross national/educational validity, and internal validity of the POP-AS.

Results

The Portuguese version of the POP-AS exhibited sound internal consistency and demonstrated adequate properties of validity: a one-factor model was obtained, revealing evidence of construct validity; invariance was ensured for education and partially ensured for the country; All the items of the POP-AS revealed relatively good values of discrimination and contributed adequately to the total score of the scale, ensuring evidence of internal validity.

Conclusion

Psychometric analysis supports the POP-AS as a valid and reliable instrument for measuring populist attitudes among Portuguese citizens. A validation framework for measurement instruments in political science was proposed. Implications of the findings are discussed.
Co-expression and clinical utility of AR-FL and AR splice variants AR-V3, AR-V7 and AR-V9 in prostate cancer
Authors: Wüstmann Neele , Seitzer Konstantin , Humberg Verena , Vieler Julia , Grundmann Norbert , Steinestel Julie , Tiedje Dorothee , Duensing Stefan , Krabbe Laura-Maria , Bögemann Martin , Schrader Andres Jan , Bernemann Christof , Schlack Katrin
Published: April 2023

Background

Androgen receptor (AR) splice variants (AR-Vs) have been discussed as a biomarker in prostate cancer (PC). However, some reports question the predictive property of AR-Vs. From a mechanistic perspective, the connection between AR full length (AR-FL) and AR-Vs is not fully understood. Here, we aimed to investigate the dependence of AR-FL and AR-V expression levels on AR gene activity. Additionally, we intended to comprehensively analyze presence of AR-FL and three clinically relevant AR-Vs (AR-V3, AR-V7 and AR-V9) in different stages of disease, especially with respect to clinical utility in PC patients undergoing AR targeted agent (ARTA) treatment.

Methods

AR-FL and AR-V levels were analyzed in PC and non-PC cell lines upon artificial increase of AR pre-mRNA using either drug treatment or AR gene activation. Furthermore, expression of AR-FL and AR-Vs was determined in PC specimen at distinct stages of disease (primary (n = 10) and metastatic tissues (n = 20), liquid biopsy samples (n = 422), mCRPC liquid biopsy samples of n = 96 patients starting novel treatment). Finally, baseline AR-FL and AR-V status was correlated with clinical outcome in a defined cohort of n = 65 mCRPC patients undergoing ARTA treatment.

Results

We revealed rising levels of AR-FL accompanied with appearance and increase of AR-Vs in dependence of elevated AR pre-mRNA levels. We also noticed increase in AR-FL and AR-V levels throughout disease progression. AR-V expression was always associated with high AR-FL levels without any sample being solely AR-V positive. In patients undergoing ARTA treatment, AR-FL did show prognostic, yet not predictive validity. Additionally, we observed a substantial clinical response to ARTA treatment even in AR-V positive patients. Accordingly, multivariate analysis did not demonstrate independent significance of AR-Vs in neither predictive nor prognostic clinical utility.

Conclusion

We demonstrate a correlation between AR-FL and AR-V expression during PC progression; with AR-V expression being a side-effect of elevated AR pre-mRNA levels. Clinically, AR-V positivity relies on high levels of AR-FL, making cells still vulnerable to ARTA treatment, as demonstrated by AR-FL and AR-V positive patients responding to ARTA treatment. Thus, AR-FL and AR-V might be considered as a prognostic, yet not predictive biomarker in mCRPC patients.
The comparative effects of consciousness raising tasks and dynamic assessment on morphological awareness: the case of pre-intermediate EFL learners
Authors: Tashmuradova Buvsara , Prasad K. D. V. , Ahmed Abdel-Al Ibrahim Khaled , Sarabani Leeda
Published: April 2023

Finding out which teaching strategies best support students learning is a key objective of educational research. Knowing this, this study investigated the comparative effects of consciousness-raising (CR) tasks and dynamic assessment (DA) on morphological awareness in an Afghani EFL context. To achieve this, 90 EFL grade 11 learners from three intact classes were selected through a convenience sampling procedure and were assigned to DA, CR, and control groups, respectively, with the same number of participants in each condition. A nonrandomized pretest–posttest design was carried out. The results of the Oxford quick placement test revealed that the participants had pre-intermediate English proficiency. To assess learners’ morphological awareness, two different tests of morphological awareness were given both prior to the treatment and right after it. The DA group received interactionist DA on the target morphemes, the CR group received CR instruction on the target form, and the control group received nothing. The results of a one-way between-groups ANOVA revealed that both DA and CR groups significantly outperformed the control group on the post-test. A further post hoc analysis using Bonferroni adjustment demonstrated that the difference between DA and CR groups was also statistically significant with a small effect size (eta squared = 0.042), substantiating the superiority of DA over CR tasks on morphological awareness. The theoretical and pedagogical implications of the study are discussed.
Frequency and correlates of lifetime suicidal ideation and suicide attempts among consecutively hospitalized youth with anorexia nervosa and bulimia nervosa: results from a retrospective chart review
Authors: Arnold Sabine , Correll Christoph U. , Jaite Charlotte
Published: April 2023

Background

Youth with eating disorders (EDs) face an increased risk of a premature suicide death. Precursors of completed suicide are suicidal ideation and suicide attempts, which need to be well understood to prevent suicide. However, epidemiological data on the lifetime prevalence and clinical correlates of suicidal ideation and suicide attempts (i.e., “suicidality”) are lacking for the vulnerable group of inpatient ED youth.

Methods

This retrospective chart review was conducted at a psychiatric child and adolescent inpatient department, covering a 25-year period. Consecutively hospitalized youth with an ICD-10 diagnosis of anorexia nervosa (AN), restricting type (AN-R), binge-purging type (AN-BP), and bulimia nervosa (BN) were included. Data extraction and coding were standardized with trained raters extracting information from patient records according to a procedural manual and using a piloted data extraction template. The lifetime prevalence of suicidal ideation and suicide attempts was calculated for each ED subgroup, and clinical correlates of suicidality were analyzed via multivariable regression analyses.

Results

In the sample of 382 inpatients aged 9–18 years (median age = 15.6, females = 97.1%; AN-R: n = 242, BN: n = 84, AN-BP: n = 56), 30.6% of patients had lifetime suicidal ideation (BN:52.4% ≈ AN-BP:44.6% > AN-R:19.8%, χ2(2,382) = 37.2, p < 0.001, Φ = 0.31), and 3.4% of patients reported a history of suicide attempts (AN-BP:8.9% ≈ BN:4.8% > AN-R:1.7%, χ2(2,382) = 7.9, p = 0.019, Φ = 0.14). Independent clinical correlates of suicidality were i) for AN-R a higher number of psychiatric comorbidities (OR = 3.02 [1.90, 4.81], p < 0.001), and body weight < 1st BMI percentile at hospital admission (OR = 1.25 [1.07,1.47], p = 0.005) (r2 = 0.20); ii) for AN-BP patients a higher number of psychiatric comorbidities (OR = 3.68 [1.50, 9.04], p = 0.004) and history of childhood abuse (OR = 0.16 [0.03, 0.96], p = 0.045) (r2 = 0.36), and iii) for BN patients a higher prevalence of non-suicidal self-injury (NSSI)(OR = 3.06 [1.37, 6.83], p = 0.006) (r2 = 0.13).

Conclusions

About half of youth inpatients with AN-BP and BN had lifetime suicidal ideation, and one-tenth of patients with AN-BP had attempted suicide. Treatment programs need to address specific clinical correlates of suicidality, namely, low body weight, psychiatric comorbidities, history of childhood abuse, and NSSI.

Trial registration

This study was not a clinical trial but a retrospective chart review based on routinely assessed clinical parameters. The study includes data from human participants; however: (1) no intervention and no prospective assignment to interventions were performed, and (2) no evaluation of intervention in participants was accomplished.
Automatic recognition of craquelure and paint loss on polychrome paintings of the Palace Museum using improved U-Net
Authors: Yuan Quan , He Xiang , Han Xiangna , Guo Hong
Published: April 2023

Craquelure is the most common defect on ancient polychrome paintings, which may deteriorate further to paint loss. Previous image processing methods, which can accurately recognize paint loss, have limited precision and efficiency in segmenting craquelure. This paper proposes a semantic segmentation method, Res-UNet, for the recognition of craquelure and paint loss in the Palace Museum, Beijing. The residual structure of ResNet-50 enables the avoidance of network degradation, and image features can be fully extracted. Using the unique skip connection module of U-Net, features of different levels are fused to improve segmentation accuracy and provide smoother craquelure edges. Three loss functions are combined to accelerate stable convergence. The model was tested on a newly built dataset based on 600 images. Experimental results supported by statistical tests show that Res-UNet is a capable method of craquelure recognition, with an accuracy rate of 98.19%, and F1-score of 93.42%. Hence, the proposed hybrid approach is a promising tool to support the preservation and restoration of valuable traditional Chinese polychrome architectural paintings.
Blood volume in patients likely to be preload responsive: a post hoc analysis of a randomized controlled trial
Authors: Lindén Anja , Statkevicius Svajunas , Bonnevier Johan , Bentzer Peter
Published: April 2023

Background

Preload responsive postoperative patients with signs of inadequate organ perfusion are commonly assumed to be hypovolemic and therefore treated with fluids to increase preload. However, preload is influenced not only by blood volume, but also by venous vascular tone and the contribution of these factors to preload responsiveness in this setting is unknown. Based on this, the objective of this study was to investigate blood volume status in preload-responsive postoperative patients.

Methods

Data from a clinical trial including postoperative patients after major abdominal surgery were analyzed. Patients with signs of inadequate organ perfusion and with data from a passive leg raising test (PLR) were included. An increase in pulse pressure by ≥ 9% was used to identify patients likely to be preload responsive. Blood volume was calculated from plasma volume measured using radiolabelled albumin and hematocrit. Patients with a blood volume of at least 10% above or below estimated normal volume were considered hyper- and hypovolemic, respectively.

Results

A total of 63 patients were included in the study. Median (IQR) blood volume in the total was 57 (50–65) ml/kg, and change in pulse pressure after PLR was 14 (7–24)%. A total of 43 patients were preload responsive. Of these patients, 44% were hypovolemic, 28% euvolemic and 28% hypervolemic.

Conclusions

A large fraction of postoperative patients with signs of hypoperfusion that are likely to be preload responsive, are hypervolemic. In these patients, treatments other than fluid administration may be a more rational approach to increase cardiac output.

Trial registration EudraCT 2013-004446-42
Effects of potassium on hydrothermal carbonization of sorghum bagasse
Authors: Yoshimoto Shuhei , Luthfi Numan , Nakano Kanta , Fukushima Takashi , Takisawa Kenji
Published: April 2023

Hydrothermal carbonization (HTC) reacts with biomass in water at a high temperature and pressure to produce hydrochar with a higher heating value (HHV) and lower ash content than dry torrefaction. The high potassium content in biomass can promote thermochemical conversion; however, it lowers the melting temperature of the ash, causing slugging and fouling. Therefore, this study, investigated the effect of potassium on the HTC of sorghum bagasse by comparing the removal of potassium by washing with the addition of K2CO3. Consequently, the ash content was the highest in the potassium-added hydrochar and was 3.81% at a reaction time of 2 h. Elemental analysis showed that the lower the potassium content, the higher the carbon content, and the hydrochar with potassium removed by water washing at a reaction time of 3 h had the highest carbon content at 68.3%. Fourier transform infrared spectrometer showed dehydration and decarboxylation reactions due to HTC, but no significant differences were observed between the potassium concentrations. The mass yield decreased with increasing potassium content, and was 27.2% for the potassium-added hydrochar after 3 h. This trend was more pronounced with increasing reaction temperature. On the other hand, HHV was not affected by the potassium content. Therefore, the energy yield was similar to the weight yield. Thermal gravimetry and derivative thermal gravimetry (TG-DTG) analysis showed that higher potassium tended to accelerate the decomposition of lignin and decrease the oxidation temperature.

Graphical Abstract
Feasibility of a theoretically grounded, multicomponent, physiotherapy intervention aiming to promote autonomous motivation to adopt and maintain physical activity in patients with lower-limb osteoarthritis: protocol for a single-arm trial
Authors: Willett Matthew , Rushton Alison , Stephens Gareth , Fenton Sally , Rich Sarah , Greig Carolyn , Duda Joan
Published: April 2023

Background

Lower-limb osteoarthritis (OA) causes high levels of pain and disability in adults over 45 years of age. Adopting and maintaining appropriate levels of physical activity (PA) can help patients with lower-limb OA self-manage their symptoms and reduce the likelihood of developing secondary noncommunicable diseases. However, patients with lower-limb OA are less active than people without musculoskeletal pain. This single-arm feasibility trial seeks to determine the feasibility and acceptability of a complex multicomponent physiotherapy behaviour change intervention that aims to aid patients with lower-limb OA to adopt and maintain optimal levels of PA.

Methods

This trial will be conducted at one site in a National Health Service physiotherapy outpatient setting in the West Midlands of England. Up to thirty-five participants with lower-limb OA will be recruited to receive a physiotherapy intervention of six sessions that aims to optimise their PA levels during phases of behavioural change: adoption, routine formation and maintenance. The intervention is underpinned by self-determination theory (and other motivational frameworks) and seeks to foster a motivationally optimal (empowering) treatment environment and implement behaviour change techniques (BCTs) that target PA behaviours across the three phases of the intervention. Physiotherapists (n = 5–6) will receive training in the why and how of developing a more empowering motivational environment and the delivery of the intervention BCTs. Participants will complete patient-reported and performance-based outcome measures at baseline and 3-month (to reflect behavioural adoption) and 6-month (maintenance) post-baseline. Feasibility and acceptability will be primarily assessed through semi-structured interviews (purposively recruiting participants) and focus groups (inviting all physiotherapists and research staff). Further evaluation will include descriptive analysis of recruitment rates, loss of follow-up and intervention fidelity.

Discussion

A novel complex, multicomponent theoretical physiotherapy behaviour change intervention that aims to create a more empowering motivational treatment environment to assist patients with lower-limb OA to adopt and maintain optimal PA levels has been developed. Testing the feasibility and acceptability of the intervention and its associated physiotherapist training and related trial procedures is required to determine whether a full-scale parallel group (1:1) randomised controlled trial to evaluate the interventions effectiveness in clinical practice is indicated.

Trial registration

Trial register: International Standard Randomised Controlled Trial identification number:ISRCTN12002764.

Date of registration: 15 February 2022.
Between here and there: comparing the worry about the pandemic between older Italian international migrants and natives in Switzerland
Authors: Ludwig-Dehm Sarah M. , Dones Iuna , Ciobanu Ruxandra Oana
Published: April 2023

Since the beginning of the pandemic in 2020, people have been worried about COVID-19. As one of the risk groups, persons aged 65 and older are especially vulnerable. Additionally, minorities and migrants are hit harder by the pandemic than natives. Using data from the TransAge survey, a study including over 3000 older persons (65+) living in Switzerland and Italy, we show that the levels of worry about the pandemic are significantly higher among Italian international migrants living in Switzerland than among Swiss natives. We are not able to fully explain the difference using sociodemographic variables, the COVID-19 situation at the time of the interview, and international migrants’ transnationalism behavior. Nevertheless, transnationalism explains a large part of the difference in worry between the two groups and our study sheds light on the importance of two specific transnational aspects, having Swiss nationality and voting behavior, for the prevention of elevated levels of worry of international migrants.
Responsiveness and minimal important change of seven PROMIS computerized adaptive tests (CAT) in patients with advanced chronic kidney disease
Authors: Terwee Caroline B. , van der Willik Esmee M. , van Breda Fenna , van Jaarsveld Brigit C. , van de Putte Marlon , Jetten Isabelle W. , Dekker Friedo W. , Meuleman Yvette , van Ittersum Frans J.
Published: April 2023

Background

The Patient-Reported Outcomes Measurement Information System (PROMIS®) has the potential to harmonize the measurement of health-related quality of life (HRQL) across medical conditions. We evaluated responsiveness and minimal important change (MIC) of seven Dutch-Flemish PROMIS computerized adaptive tests (CAT) in Dutch patients with advanced chronic kidney disease (CKD).

Methods

CKD patients (eGFR < 30 ml/min.1.73m2) completed at baseline and after 6 months seven PROMIS CATs (assessing physical function, pain interference, fatigue, sleep disturbance, anxiety, depression, and ability to participate in social roles and activities), Short Form Health Survey 12 (SF-12), PROMIS Pain Intensity single item, Dialysis Symptom Index (DSI), and Global Rating Scales (GRS) of change. Responsiveness was assessed by testing predefined hypotheses about expected correlations among measures, area under the ROC Curve, and effect sizes. MIC was determined with predictive modelling.

Results

207 patients were included; 186 (90%) completed the follow-up. Most results were in accordance with expectations (70–91% of hypotheses confirmed), with some exceptions for PROMIS Anxiety and Ability to Participate (60% and 42% of hypotheses confirmed, respectively). For PROMIS Anxiety and Depression correlations with the GRS were too low (0.04 and 0.20, respectively) to calculate a MIC. MIC values, representing minimal important deterioration, ranged from 0.4 to 2.5 T-score points for the other domains.

Conclusion

We found sufficient responsiveness of PROMIS CATs Physical Function, Fatigue, Sleep Disturbance, and Depression. The results for PROMIS CATs Pain Interference were almost sufficient, but some results for Anxiety and Ability to Participate in Social Roles and Activities were not as expected. Reported MIC values should be interpreted with caution because most patients did not change.
Examining socioeconomic factors associated with wildfire occurrence and burned area in Galicia (Spain) using spatial and temporal data
Authors: de Diego Jaime , Fernández Mercedes , Rúa Antonio , Kline Jeffrey D.
Published: April 2023

Background

The Spanish region of Galicia is one of the most fire-prone areas in Europe. Most wildfires are directly or indirectly related to human activities, suggesting that socioeconomic factors likely can inform wildfire management. Socioeconomic factors, for example, could help explain the causes and distribution of wildfires spatially and temporally within the region. We sought to examine how socioeconomic and biophysical variables and their fluctuations over time (2000–2015) might influence wildfire ignitions and hectares burned in Galicia using municipality-level data describing socioeconomic factors and forest land wildfires.

Results

We used cluster analysis to characterize socioeconomic variation in our data set and regression analysis of panel data to investigate jointly the temporal and spatial dimensions of correlation between socioeconomic factors and wildfires. Using cluster analysis, we divided the region inter-territorially according to socioeconomic characteristics. Clustering divisions were found to have a similar pattern to that of Galician provinces. Our regression models for each cluster indicate that several socioeconomic factors are at least correlated with and may tend to influence wildfire occurrence and burned area in Galicia. We also found discernible patterns related to our identified clusters, confirming that differences between territories exist regarding the likely influence of socioeconomic factors on the number of wildfire ignitions and hectares burned.

Conclusions

Results suggest that socioeconomic factors are as crucial as meteorological variables for understanding wildfire ignitions and burned area in Galicia (Spain). Accurate knowledge of inter-territorial socioeconomic differences could help public officials and forest managers to design wildfire prevention policies best suited to the socioeconomic, cultural, and environmental circumstances of each territory.
Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater
Authors: Agboola Jeleel O. , Rocha Sérgio D. C. , Mensah Dominic D. , Hansen Jon Ø. , Øyås Ove , Lapeña David , Mydland Liv T. , Arntzen Magnus Ø. , Horn Svein J. , Øverland Margareth
Published: April 2023

Background

Yeasts are gaining attention as alternative ingredients in aquafeeds. However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited. Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet. Two yeasts,Cyberlindnera jadinii(CJ) andWickerhamomyces anomalus(WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA). In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA). Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.

Results

The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet. The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets). Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish. The gut microbiota of fish fed ACJ diet was dominated by the genusPediococcusand showed a predicted increase in mucin O-glycan degradation compared with the other diets. The gut microbiota of fish fed AWA diet was highly dominated by the familyBacillaceae.

Conclusions

The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish. The inclusion of inactivated yeasts did not alter the modulation caused by SBM-based diet. Fish fed ACJ diet increased relative abundance ofPediococcus,and mucin O-glycan degradation pathway compared with the other diets.
Preoperative prediction of Ki-67 status in invasive breast carcinoma using dynamic contrast-enhanced MRI, diffusion-weighted imaging and diffusion tensor imaging
Authors: EL-Metwally Dina , Monier Dalia , Hassan Amany , Helal Amira Mohamed
Published: April 2023

Background

The Ki-67 is a beneficial marker of tumor aggressiveness. It is proliferation index that has been used to distinguish luminal B from luminal A breast cancers. By fast progress in quantitative radiology modalities, tumor biology and genetics can be assessed in a more accurate, predictive, and cost-effective method. The aim of this study was to assess the role of dynamic contrast-enhanced magnetic resonance imaging, diffusion-weighted imaging and diffusion tensor imaging in prediction of Ki-67 status in patients with invasive breast carcinoma estimate cut off values between breast cancer with high Ki-67 status and those with low Ki-67 status.

Results

Cut off ADC (apparent diffusion co-efficient) value of 0.657 mm2/s had 96.4% sensitivity, 75% specificity and 93.8% accuracy in differentiating cases with high Ki67 from those with low Ki67. Cut off maximum enhancement value of 1715 had 96.4% sensitivity, 75% specificity and 93.8% accuracy in differentiating cases with high Ki67 from those with low Ki67. Cut off washout rate of 0.73 I/S had 60.7% sensitivity, 75% specificity and 62.5% accuracy in differentiating cases with high Ki67 from those with low Ki67. Cut off time to peak value of 304 had 71.4% sensitivity, 75% specificity and 71.9% accuracy in differentiating cases with high Ki67 from those with low Ki67.

Conclusions

ADC, time to peak and maximum enhancement values had high sensitivity, specificity and accuracy in differentiating breast cancer with high Ki-67 status from those with low Ki-67 status.
Creating implementable clinical practice guidelines: the 2020 Focused Updates to the National Heart, Lung, and Blood Institute’s Asthma Management Guidelines
Authors: Shero Susan T. , Ammary-Risch Neyal J. , Lomotan Edwin A. , Mardon Russell E. , Michaels Maria
Published: April 2023

Background

The 2020 Focused Updates to the Asthma Management Guidelines: A Report from the National Asthma Education and Prevention Program Coordinating Committee Expert Panel Working Group provides the first new clinical practice recommendations from the National Heart, Lung, and Blood Institute (NHLBI) since the previous 2007 asthma management guidelines. Guideline implementability was a high priority for the expert panel, and many approaches were undertaken to enhance the implementability of this clinical guideline update.

Within the report, specific implementation guidance sections provide expanded summaries for each recommendation to quickly assist users. The implementation guidance incorporates findings from NHLBI-sponsored focus groups conducted with people who have asthma, caregivers, and health care providers. The findings were used to identify the types of information and tools that individuals with asthma, their caregivers, and their health care providers would find most helpful; ensure that the new asthma guidelines reflect the voices of individuals with asthma and their caregivers; and identify potential barriers to uptake by individuals with asthma and their caregivers. The expert panel used a GRADE-based approach to develop evidence-to-decision tables that provided a framework for assessing the evidence and consideration of a range of contextual factors that influenced the recommendations such as desirable and undesirable effects, certainty of evidence, values, balance of effects, acceptability, feasibility, and equity. To facilitate uptake in clinical care workflow, selected recommendations were converted into structured, computer-based clinical decision support artifacts, and the new recommendations were integrated into existing treatment tables used in the 2007 asthma management guidelines, with which many users are familiar. A comprehensive approach to improve guidelines dissemination and implementation included scientific publications, patient materials, media activities, stakeholder engagement, and professional education.

Conclusion

We developed evidence-based clinical practice guideline updates for asthma management focused on six topic areas. The guideline development processes and implementation and dissemination activities undertaken sought to enhance implementability by focusing on intrinsic factors as described by Kastner, Gagliardi, and others to produce usable, adoptable, and adaptable guidelines. Enhanced collaboration during guideline development between authors, informaticists, and implementation scientists may facilitate the development of tools that support the application of recommendations to further improve implementability.
Study of serum bisphenol-A and the mRNA of galactosidase beta 1 and tumor necrosis factor alpha in Egyptian patients with type 2 diabetes mellitus
Authors: Zanet Yasmin Ibrahim , Hashem Enayat Mohamed , Dessouky Mohammed Naguib , Megalla Magdy Helmy , Dessouky Iman Samy
Published: April 2023

Background

The pathogenesis of type 2 diabetes mellitus (T2DM) originates from multiple tissue defects. Environmental pollutants such like endocrine-disrupting chemicals (EDCs) have lately gotten special attention in triggering the metabolic abnormalities in relation to insulin resistance and T2DM. Bisphenol-A (BPA), which is one of the EDCs and simulates natural endogenous estrogens, has been implicated in the pathogenesis of several diseases, and, over the last decade, it has been revealed to have probable diabetogenic and obesogenic actions.

Aim

To study the environmental exposure of BPA by measuring its serum level together with the relative expression level of galactosidase beta 1 (GLB1), tumor necrosis alpha (TNF-α) mRNAs and malondialdehyde (MDA) as an oxidative stress marker determine the association of these markers with the glycemic control of Egyptian patients having T2DM.

Patients and methods

The present study was conducted on 90 participants categorized into group 1: 60 patients with T2DM and group 2: 30 subjects with normal glucose tolerance. Serum BPA levels were measured by enzyme-linked immunosorbent assay (ELISA) kit, and biochemical determinations were done by standard protocols. Because of the pro-oxidizing role of BPA, we measured serum level of malondialdehyde (MDA) as an oxidative stress marker. Peripheral blood mononuclear cells (PBMCs) were used for detecting the gene expression changes in GLB1 mRNA, a major marker of cellular senescence, and the mRNA of the proinflammatory cytokine, TNF-α.

Results

Serum levels of BPA in T2DM patients were significantly higher compared to their control group, and they were significantly and positively correlated to poor glycemic control and insulin resistance. T2DM patients showed significantly high serum MDA, mRNA levels of GLB1, and TNF-α. There was positive correlation between serum BPA levels and oxidative stress marker, senescence indicator, and inflammatory marker; however, there was no statistical significance for this correlation.

Conclusion

Our study demonstrates that there is a link between increased serum BPA levels, poor glycemic control, and insulin resistance in Egyptian patients with T2DM.
Statistical Approaches for Establishing Appropriate Immunogenicity Assay Cut Points: Impact of Sample Distribution, Sample Size, and Outlier Removal
Authors: Garlits John , McAfee Sean , Taylor Jessica-Ann , Shum Enoch , Yang Qi , Nunez Emily , Kameron Kristina , Fenech Keilah , Rodriguez Jacqueline , Torri Albert , Chen Jihua , Sumner Giane , Partridge Michael A.
Published: April 2023

The statistical assessments needed to establish anti-drug antibody (ADA) assay cut points (CPs) can be challenging for bioanalytical scientists. Poorly established CPs that are too high could potentially miss treatment emergent ADA or, when set too low, result in detection of responses that may have no clinical relevance. We evaluated 16 validation CP datasets generated with ADA assays at Regeneron’s bioanalytical laboratory and compared results obtained from different CP calculation tools. We systematically evaluated the impact of various factors on CP determination including biological and analytical variability, number of samples for capturing biological variability, outlier removal methods, and the use of parametric vs. non-parametric CP determination. In every study, biological factors were the major component of assay response variability, far outweighing the contribution from analytical variability. Non-parametric CP estimations resulted in screening positivity in drug-naïve samples closer to the targeted rate (5%) and were less impacted by skewness. Outlier removal using the boxplot method with an interquartile range (IQR) factor of 3.0 resulted in screening positivity close to the 5% targeted rate when applied to entire drug-naïve dataset. In silico analysis of CPs calculated using different sample sizes showed that using larger numbers of individuals resulted in CP estimates closer to the CP of the entire population, indicating a larger sample size (~ 150) for CP determination better represents the diversity of the study population. Finally, simpler CP calculations, such as the boxplot method performed in Excel, resulted in CPs similar to those determined using complex methods, such as random-effects ANOVA.

Graphical Abstract
Intraoperative Electroencephalography Alpha-Band Power Is a Better Proxy for Preoperative Low MoCA Under Propofol Compared With Sevoflurane
Authors: Guessous K. , Touchard C. , Glezerson B. , Levé C. , Sabbagh D. , Mebazaa A. , Gayat E. , Paquet C. , Vallée F. , Cartailler J.
Published: April 2023

BACKGROUND:

Preoperative abnormal cognitive status is a risk factor for postoperative complications yet remains underdiagnosed. During propofol general anesthesia, intraoperative electroencephalography (EEG) variables, such as alpha band power (α-BP), correlate with cognitive status. This relationship under sevoflurane is unclear. We investigated whether EEG biomarkers of poor cognitive status found under propofol could be extended to sevoflurane.

METHODS:

In this monocentric prospective observational study, 106 patients with intraoperative EEG monitoring were included (propofol/sevoflurane = 55/51). We administered the Montreal Cognitive Assessment (MoCA) scale to identify abnormal cognition (low MoCA) 1 day before intervention. EEG variables included delta to beta frequency band powers. Results were adjusted to age and drug dosage. We assessed depth of anesthesia (DoA) using the spectral edge frequency (SEF95) and maintained it within (8–13) Hz.

RESULTS:

The difference in α-BP between low and normal MoCA patients was significantly larger among propofol patients (propofol: 4.3 ± 4.8 dB versus sevoflurane: 1.5 ± 3.4 dB, P = .022). SEF95 and age were not statistically different between sevoflurane and propofol groups. After adjusting to age and dose, low α-BP was significantly associated with low MoCA under propofol (odds ratio [OR] [confidence interval {CI}] = 0.39 [0.16–0.94], P = .034), but not under sevoflurane, where theta-band power was significantly associated with low MoCA (OR [CI] = 0.31 [0.13–0.73], P = .007).

CONCLUSIONS:

We suggest that intraoperative EEG biomarkers of abnormal cognition differ between propofol and sevoflurane under general anesthesia.
Artists in the Archives
Authors: Cvetkovich Ann
Published: April 2023

Ulrike Müller's Herstory Inventory (HI) is a collection of over one hundred works on paper by “feminist” artists who were given “drawing assignments” that began with textual prompts taken from an archival list of T-shirts that Müller discovered in the collections of the Lesbian Herstory Archives (LHA). HI has also had multiple incarnations as a staged reading/live performance, audio installation, collective art project, art exhibition, and book, and its relay across media participates in a fascination with the archive that has pervaded LGBTQ culture, resulting in a proliferation of new archives that is one manifestation of the “archival turn.” This essay focuses on how Müller's HI uses the LHA as a point of departure for a creative practice that not only opens lesbian feminist archives to new visibility and new publics but also creates a transgenerational dialogue around lesbian feminist politics and representation — both honoring and reviving its history and subjecting it to critique. HI's engagement with the LHA's lesbian feminist commitment to archival autonomy provides an interesting case history for radical archival politics, as tensions between counterarchives and archival critique get played out through the tensions between lesbian and queer feminisms. Returning to the politics of representation and visibility that have been so central and vexing in lesbian feminism, HI puts art practices in conversation with archival ones. The project approaches the archive through abstraction and drawing, both practices of representation that resist the realisms of documentary media such as film and photography, to enact a queer politics of visibility.
Cellular effects of NAT-mediated histone N-terminal acetylation
Authors: Constantinou Mamantia , Klavaris Ariel , Koufaris Costas , Kirmizis Antonis
Published: April 2023

Histone acetylation involves the addition of acetyl groups to specific amino acid residues. This chemical histone modification is broadly divided into two types – acetylation of the amino group found on the side chain of internal lysine residues (lysine acetylation) or acetylation of the α-amino group at the N-terminal amino acid residue (N-terminal acetylation). Although the former modification is considered a classic epigenetic mark, the biological importance of N-terminal acetylation has been mostly overlooked in the past, despite its widespread occurrence and evolutionary conservation. However, recent studies have now conclusively demonstrated that histone N-terminal acetylation impacts important cellular processes, such as controlling gene expression and chromatin function, and thus ultimately affecting biological phenotypes, such as cellular ageing, metabolic rewiring and cancer. In this Review, we provide a summary of the literature, highlighting current knowledge on the function of this modification, as well as allude to open questions we expect to be the focus of future research on histone N-terminal acetylation.