bluuebunny/crossref_metadata_2025 huggingface dataset:
Thermoresponsive M1 macrophage-derived hybrid nanovesicles for improved in vivo tumor targeting
Authors: Barone Antonella , Zimbo Anna Maria , d’Avanzo Nicola , Tolomeo Anna Maria , Ruga Stefano , Cardamone Antonio , Celia Christian , Scalise Mariangela , Torella Daniele , La Deda Massimo , Iaccino Enrico , Paolino Donatella
Published: June 2023

Despite the efforts and advances done in the last few decades, cancer still remains one of the main leading causes of death worldwide. Nanomedicine and in particular extracellular vesicles are one of the most potent tools to improve the effectiveness of anticancer therapies. In these attempts, the aim of this work is to realize a hybrid nanosystem through the fusion between the M1 macrophages-derived extracellular vesicles (EVs-M1) and thermoresponsive liposomes, in order to obtain a drug delivery system able to exploit the intrinsic tumor targeting capability of immune cells reflected on EVs and thermoresponsiveness of synthetic nanovesicles. The obtained nanocarrier has been physicochemically characterized, and the hybridization process has been validated by cytofluorimetric analysis, while the thermoresponsiveness was in vitro confirmed through the use of a fluorescent probe. Tumor targeting features of hybrid nanovesicles were in vivo investigated on melanoma-induced mice model monitoring the accumulation in tumor site through live imaging and confirmed by cytofluorimetric analysis, showing higher targeting properties of hybrid nanosystem compared to both liposomes and native EVs. These promising results confirmed the ability of this nanosystem to combine the advantages of both nanotechnologies, also highlighting their potential use as effective and safe personalized anticancer nanomedicine.

Graphical Abstract
High Adsorption Capacity of Ammonia Gas Pollutant Using Adsorbents of Carbon Composites
Authors: Mohamed Elham F. , El-Mekawy Asmaa , Ahmed Sohair A. Sayed , Fathy Nady A.
Published: June 2023

Air pollution is one of the most environmentally harmful to the human health and the climate change. The present study is aimed to investigate the effectiveness and capability of novel composite adsorbents prepared from modification of activated carbons (ACs) in the removal of air pollutants. The effect of both triethoxysilane propylamine (TEPSA) and carbon nanotube (CNT) on the adsorption properties of AC (TEPSA/CNT/AC); in addition, silica gel prepared from TEPSA to form silica nanoparticles (SiNP) along with CNT and AC (SiNP/CNT/AC), was studied. Ammonia gas (NH3) was used in this study as a typical emerging gas air pollutant. The physicochemical characteristics of the prepared ACs samples were analyzed using BET surface area, SEM, EDX, TEM and FTIR. Their results proved considerable changes in the porosity and surface functional groups after modifying AC surface with TEPSA or SiNP and CNT instantly. The adsorption findings showed that the NH3removal efficiency using the prepared AC samples reached almost 97, 86 and 75% during 90 min at 25 °C for each sample of SiNP/CNT/AC, TESPA/CNT/AC and AC, respectively. Adsorption of NH3relied mainly on the presence of silica particles and mesopore distributions in CNT rather than large total surface area in AC alone. Thus, the combination of SiNP as silanol and siloxane groups with CNT on AC surface raised significantly the adsorption capacity of NH3from 194 to 300 mg/g. Conclusively, the SiNP/CNT/AC sample exhibited the best performance among all prepared samples used for the adsorption of NH3gas from the indoor air. Also, Langmuir and Freundlich models were applied, and the results revealed that Freundlich model fits well the equilibrium adsorption data.
A qualitative comparison of the intended curriculum ideologies of STEM subjects in South Africa
Authors: Ramulumo Moleboheng , Mnguni Lindelani
Published: June 2023

The democratically elected government of South Africa has enacted several curricular reforms which sought to promote citizenship education. By instituting citizenship education, the government intended to promote student engagement in decision-making, rebuilding, and emancipation of society. The curriculum would facilitate the training of students to become logical thinkers and informed citizens who actively participate in rectifying the injustices and inequalities of the apartheid state. However, a curriculum ideology foregrounding citizenship education has not been extensively understood, especially in STEM (science, technology, engineering, mathematics) education. In the current study, we investigated the intended ideologies foregrounding the curriculum of three purposively selected subjects: Natural Sciences, Life Sciences, and Physical Sciences. While Life Sciences and Physical Sciences are taught in grades 10 to 12, Natural Sciences is compulsory and taught in grades 7 to 9. We used structured document analysis to analyze the curriculum documents qualitatively. We discovered evidence of a combination of citizenship-centered, service-centered, discipline-centered, and student-centered ideologies in the subjects to varying degrees. We found neither a single dominant ideology nor evidence of coherence and integration of these ideologies. Given the lack of a dominant curriculum ideology, we argue that teachers and other educational stakeholders may interpret and use the curriculum differently, leading to an “ideological war.” We conclude that these science subjects are unlikely to promote citizenship education as the South African government intends.
The Macro-Political Context and Interest Groups' Access to Policymakers
Authors: Labanino Rafael , Dobbins Michael
Published: June 2023

The article explores how macro-level political factors in conjunction with micro- and meso-level factors affect interest-group access to policymakers. The analysis is conducted based on two original data sets: a population ecology database of Czech, Hungarian, Polish and Slovenian national-level energy policy, healthcare and higher education organizations, and an online survey of these populations. Combining the two data sets allows us to investigate both polity-, population- and organizational-level factors. As the sampled countries have recently experienced democratic backsliding, we also test the effect of closing deliberative structures. The analysis reveals that the political process influences access: legislative fractionalization affects access positively, while the closure of deliberative structures has a negative effect. Nevertheless, the political contextual factors are mediated through variables at both the population (e.g. the size of latent constituency) and organizational (e.g. expertise provision) levels, as well as the meso-level of interorganizational cooperation.
Cold tolerance and overwintering survival of Aphelinus certus (Hymenoptera: Aphelinidae), a parasitoid of the soybean aphid (Hemiptera: Aphididae) in North America
Authors: Stenoien Carl M. , Christianson Lindsey , Welch Kelton , Dregni Jonathan , Hopper Keith R. , Heimpel George E.
Published: June 2023

Broad-spectrum insecticides are the main control measure of the invasive and economically damaging soybean aphid (Aphis glycines) in North America, although biological control by resident natural enemies can also greatly diminish population levels. One such natural enemy is the accidentally introduced Eurasian parasitoid Aphelinus certus (Hymenoptera: Aphelinidae), though its impact appears to be limited by low rates of parasitism early in the growing season. We tested the hypothesis that A. certus might experience high overwintering mortality. In the laboratory, we used thermocouple thermometry to measure the supercooling points of diapausing parasitoids and assessed parasitoid survival after exposure to ecologically relevant durations of low temperature. We found A. certus to be freeze-intolerant with a median supercooling point of −28°C. When exposed to temperatures of 0°C for up to 7 months, adults emerged only after exposures of at least 60 days and survival decreased with durations beyond 150 days. We also conducted in-field studies at sites from northern Minnesota to southern Iowa to determine if diapausing A. certus could overwinter above and below the snowpack. Survival was negatively correlated with increasing latitude and was greater for parasitoids placed on the ground than 1 meter off the ground, likely due to the warmer and stabler temperatures of the subnivean microclimate. Our results suggest that A. certus is capable of overwintering in the region inhabited by soybean aphid but may experience substantial mortality even under ideal conditions. Climate change is predicted to bring warmer, drier winters to the North American Midwest, with decreased depth and duration of snow cover, which may further reduce overwintering survival.
The problem with decolonisation: entanglements in the politics of knowledge
Authors: Jansen Jonathan
Published: June 2023

In the heat of the decolonisation struggles of the 2000s, there has been little space or tolerance for conceptual criticism of this important moment in global history. Using the South African case, this article outlines some of the dilemmas of decolonisation as a concept and method for dealing with legacy knowledge in the aftermath of colonialism and apartheid. The status of whites as citizens rather than colonials, the lack of determination of meanings of decolonisation within public universities, and the defanging of a potentially radical concept are among the concerns raised in this critical work on the uptake of the idea in post-apartheid society. What this criticism points to is the need for a theory of institutions when dealing with radical curriculum change rather than a politics that relies so much on the rhetorical, the symbolic and the performative in the demand for decolonisation.
Impact of the pandemic and its containment measures in Europe upon aspects of affective impairments: a Google Trends informetrics study
Authors: Szilagyi Istvan-Szilard , Eggeling Eva , Bornemann-Cimenti Helmar , Ullrich Torsten
Published: June 2023

Background

In late 2019, a new virus began spreading in Wuhan, China. By the end of 2021, more than 260 million people worldwide had been infected and 5.2 million people had died because of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Various countermeasures have been implemented to contain the infections, depending on the country, infection prevalence, and political and infrastructural resources. The pandemic and the containment measures have induced diverse psychological burdens. Using internet queries as a proxy, this study examines the psychological consequences on a European level of SARS-CoV-2 containment measures.

Methods

Using informetric analyses, this study reviews within 32 European countries a total of 28 search parameters derived from the International Statistical Classification of Diseases and Related Health Problems (ICD-10) as aspects of affective disorder.

Results

Our results show that there are several psychological aspects which are significantly emphasized during the pandemic and its containment measures: ‘anxiety’, ‘dejection’, ‘weariness’, ‘listlessness’, ‘loss of appetite’, ‘loss of libido’, ‘panic attack’, and ‘worthlessness’. These terms are significantly more frequently part of a search query during the pandemic than before the outbreak. Furthermore, our results revealed that search parameters such as ‘psychologist’, ‘psychotherapist’, ‘psychotherapy’ have increased highly significantly (p < 0.01) since the pandemic.

Conclusions

The psychological distress caused by the pandemic correlates significantly with the frequency of people searching for psychological and psychotherapeutic support on the Internet.
Design, modeling and solar tracking control for a novel parabolic dish solar concentrator
Authors: Mo Han , Liu Fanmao , Liao Cancan , Zhang Yuanyuan
Published: June 2023

A novel parabolic dish solar concentrator based on the improved 3-RPS parallel manipulator to drive the reflective mirror facet is proposed and designed, which can not only automatically adjust the position and orientation of the reflective mirror facet but also have the advantages of independent drive, high stiffness and no cumulative error. Then, using the coordinate transformation matrixes of the novel parabolic dish solar tracking platform, the kinematics models of the 3-RPS parallel manipulators associated with the solar altitude and azimuth angles are established. The altitude and azimuth angles of the solar movement at the installation location are calculated according to the calculation formula of solar position. To solve the problem of too many telescopic rods of the 3-RPS parallel manipulator, a genetic algorithm is used to optimize the height of the concentrator’s center of mass. Then the ideal trajectory and attitude of each telescopic rod of the 3-RPS parallel manipulators at different times of the day can be obtained with the inverse kinematics. The particle swarm optimization (PSO)-proportional-integral-derivative (PID) controller, which uses PSO algorithm to tune PID parameters, is proposed for solar trajectory tracking of the novel parabolic dish solar concentrator. The visual simulation model of the parabolic dish system is established in Simscape Multibody, and the trajectory tracking control experiment is carried out. The experimental results show that the trajectory tracking error of the novel dish solar tracking platform can be within 2.6 mm by using the PSO-PID controller.
The Implementation of a Prehospital Whole Blood Transfusion Program and Early Results
Authors: Coyle Charles , Zitek Tony , Pepe Paul E. , Stotsenburg Madonna , Scheppke Kenneth A. , Antevy Peter , Giroux Richard , Farcy David A.
Published: June 2023

Introduction:

In far-forward combat situations, the military challenged dogma by using whole blood transfusions (WBTs) rather than component-based therapy. More recently, some trauma centers have initiated WBT programs with reported success. There are a few Emergency Medical Service (EMS) systems that are using WBTs, but the vast majority are not. Given the increasing data supporting the use of WBTs in the prehospital setting, more EMS systems are likely to consider or begin WBT programs in the future.

Objective:

A prehospital WBT program was recently implemented in Palm Beach County, Florida (USA). This report will discuss how the program was implemented, the obstacles faced, and the initial results.

Methods:

This report describes the process by which a prehospital WBT program was implemented by Palm Beach County Fire Rescue and the outcomes of the initial case series of patients who received WBTs in this system. Efforts to initiate the prehospital WBT program for this system began in 2018. The program had several obstacles to overcome, with one of the major obstacles being the legal team’s perception of potential liability that might occur with a new prehospital blood transfusion program. This obstacle was overcome through education of local elected officials regarding the latest scientific evidence in favor of prehospital WBTs with potential life-saving benefits to the community. After moving past this hurdle, the program went live on July 6, 2022. The initial indications for transfusion of cold-stored, low titer, leukoreduced O+ whole blood in the prehospital setting included traumatic injuries with systolic blood pressure (SBP) < 70mmHg or SBP < 90mmHg plus heart rate (HR) > 110 beats per minute.

Findings:

From the date of onset through December 31, 2022, Palm Beach County Fire Rescue transported a total of 881 trauma activation patients, with 20 (2.3%) receiving WBT. Overall, nine (45%) of the patients who had received WBTs so far remain alive. No adverse events related to transfusion were identified following WBT administration. A total of 18 units of whole blood reached expiration of the unit’s shelf life prior to transfusion.

Conclusion:

Despite a number of logistical and legal obstacles, Palm Beach County Fire Rescue successfully implemented a prehospital WBT program. Other EMS systems that are considering a prehospital WBT program should review the included protocol and the barriers to implementation that were faced.
Objective burden, caregiver psychological distress, and patient religion and quality of life are associated with high-intensity burden of care among caregivers of advanced cancer patients in a Latino population
Authors: Soto-Guerrero Sebastián , Palacios Josefa , Langer Paola , Carrasco Cecilia , Tupper-Satt Laura , González-Otaíza Marcela , Rodríguez-Núñez Alfredo , Pérez-Cruz Pedro E.
Published: June 2023

Objectives

Family caregivers (FCs) of cancer patients experience burden of care. The aims of this study are to describe the caregiving phenomenon among FCs of advanced cancer patients in a Latino community and to identify caregiver and patient characteristics associated with high-intensity subjective caregiver burden.

Methods

In this cross-sectional study, advanced cancer patient–caregiver dyads assessed at a Palliative Care Unit in Santiago, Chile, enrolled in a longitudinal observational study were included. FCs completed questions to describe the caregiving phenomenon and surveys to assess burden of care, psychological distress, and perception of patients’ symptoms; patients completed surveys to assess physical distress and quality of life (QOL). We explored associations between high-intensity subjective caregiver burden with caregiver and patient variables.

Results

Two hundred seven dyads were analyzed. FCs were on average 50 years old and 75% female. Thirty-two percent of FCs experienced high-intensity subjective burden of care. Eighty two percent of FCs took care of the patient daily and 31% took care of the patient alone. In univariate analysis, high-intensity caregiver burden was associated with caregiver depression (59% vs. 27%; p < 0.001), anxiety (86% vs. 67%; p = 0.003), caring for the patient alone (45% vs. 24%; p = 0.002), perception of patient symptom distress, patient religion, and worse patient QOL (mean [standard deviation] 58 [33] vs. 68 [27]; p = 0.03). In multivariate analysis, FC depression (OR [95% confidence interval] 3.07 [1.43–6.60]; p = 0.004), anxiety (3.02 [1.19–7.71]; p = 0.021), caring for the patient alone (2.69 [1.26–5.77]; p = 0.011), caregiver perception of patient’s fatigue (1.26 [1.01–1.58]; p = 0.04), and patient’s religion (3.90 [1.21–12.61]; p = 0.02) were independently associated with caregiver burden.

Significance of results

FCs of advanced cancer patients in a Latino community frequently experience high-intensity burden of care and are exposed to measures of objective burden. High-intensity burden is associated with both caregiver and patient factors. Policies should aim to make interventions on patient–caregiver dyads to decrease caregiving burden among Latinos.
Emergency care reconfiguration in the Netherlands: conflicting interests and trade-offs from a multidisciplinary perspective
Authors: van Velzen Nanne , Janssen Richard , Varkevisser Marco
Published: June 2023

Many countries are reconfiguring their emergency care systems to improve quality and efficiency of care, and this often includes the concentration of emergency departments (EDs). This trend is evident in the Netherlands, but the best approach is the subject of debate among stakeholders. We (i) examined the views of stakeholders on the concentration of EDs in the Netherlands and (ii) identified the main conflicting interests and trade-offs that are relevant for health policy. To do this, we organised focus groups and semi-structured interviews with emergency care professionals, hospital executives and selected external stakeholders. First, the participants saw both advantages and disadvantages to concentration, but these were also contested and debated. Second, we found that – sometimes conflicting – public health care goals (i.e. quality, accessibility and affordability) and narrower interests (e.g. the interests of specific hospitals, insurers, medical specialists and local administrators) were both pointed out. Third, there was no clear preferred approach to the future organisation of EDs, although most stakeholders mentioned some form of centralised decision-making at the national level, combined with regional customisation. Our findings will facilitate health policy decision-making around the reconfiguration of emergency care with the long-term goal of achieving efficient and high-quality emergency care.
ISORROPIA‐MCX: Enabling Sensitivity Analysis With Multicomplex Variables in the Aerosol Thermodynamic Model, ISORROPIA
Authors: Berman Bryan , Capps Shannon L. , Sauvageau Isaiah , Gao Eric , Eastham Sebastian D. , Russell Ryan P.
Published: June 2023

Sensitivity analysis with atmospheric chemical transport models may be used to quantify influences of specific emissions on pollutant concentrations. This information facilitates efficient environmental decision‐making regarding emissions control strategies for pollutants that affect human health and public welfare. The multicomplex step method (MCX) is a sensitivity analysis approach that enables calculation of first‐ and higher‐order sensitivities of a nonlinear algorithm with analytical accuracy. Compared to the well‐known finite difference method, the MCX method is also straight‐forward to compute yet does not suffer from precision errors due to subtracting numbers with common leading digits and eliminates the requirement of tuning the step size. The aerosol inorganic equilibrium thermodynamic model, ISORROPIA, which treats ammonium, chloride, nitrate, sodium, sulfate, calcium, potassium, and magnesium, was augmented to leverage the multicomplex step method (ISORROPIA‐MCX) to analyze the influence that the total amount of a pollutant has on concentrations partitioned into different phases. This enables simultaneous calculation of the first‐order, second‐order, and cross‐sensitivity terms in the Taylor Series expansion when evaluating the impact of changes in input parameters on an output variable, increasing the accuracy of the estimated effect when the functions are nonlinear. ISORROPIA encodes highly nonlinear processes which showcases the computational advantages of the multicomplex step method as well as the limitations of the approach for fractured solution surfaces. With ISORROPIA‐MCX, the influence of total concentrations of aerosol precursors on aerosol acidity are evaluated with cross‐sensitivity terms for the first time.
Shifts of Formation Regimes and Increases of Atmospheric Oxidation Led to Ozone Increase in North China Plain and Yangtze River Delta From 2016 to 2019
Authors: Zhu Shengqiang , Ma Jinlong , Wang Siyu , Sun Shida , Wang Peng , Zhang Hongliang
Published: June 2023

China is confronting severe ozone (O3) pollution although particulate matter reduced significantly, causing damages to public health and ecological systems. Here we utilized the comprehensive methods of ground‐level observations, satellite data, and source‐oriented chemical transport model to interpret O3 variations throughout China from 2016 to 2019. A remarkably worsened trend of O3 levels has been found both by observation and simulation in these years. Our results showed that the remarkable O3 elevation was found in the North China Plain (NCP) and Yangtze River Delta (YRD) (maximum daily 8 hr average O3 ∼60 ppb) with an annual increasing rate of 10%. In addition, O3 formation regimes also changed in the NCP, where 4.9% grids shifted from VOC‐limited to transition regimes and 9.6% grids shifted from transition to NOx‐limited regimes. The elevation of MDA8 O3 was mainly attributed to the enhanced atmospheric oxidation capacity (AOC) in above regions. Particularly, the increasing rates of OH and HO2 radicals (major oxidants) in NCP and YRD reached ∼15% and ∼5%, respectively. The comprehensive study of long‐term O3 changes, formation regimes, and AOC based on a multimethod approach should be considered when designing O3 control policies.
Toward More Accurate GIC Estimations in the Portuguese Power Network
Authors: Alves Ribeiro Joana , Pinheiro Fernando J. G. , Pais Maria Alexandra , Santos Rute , Cardoso João , Baltazar‐Soares Pedro , Monteiro Santos Fernando A.
Published: June 2023

New geomagnetically induced current (GIC) computations for mainland Portugal include the entire power network, with network parameters and topology provided by the transmission grid operator for all the high voltage lines (150, 220, and 400 kV). The first 3D conductivity model for the west region of the Iberian Peninsula, based on 31 broadband magnetotelluric soundings, is used in calculations, revealing the effect of different crustal domains in GIC distribution. Geomagnetic field variations are taken from Coimbra or San Fernando magnetic observatories, according to the Nearest Neighbor method, and used together with surface impedance values predicted from the new conductivity model to calculate the induced electric field on a regular grid. The global distribution of GICs over the power network is characterized based on results derived for the eight most significant storms registered in the Iberia during solar cycle 24. Substations susceptible to the highest GICs are found near the transition between the granitic geotectonic unit of Central Iberian Zone and the Lusitanian Basin. A prototype of a Hall effect sensor has been installed at a substation and is active since the end of August 2021. In order to validate our GIC model, recent measurements are compared with simulations. GIC computation is prone to uncertainties from various sources, possibly contributing with different weights to the final error in computed values. Here, we evaluate the contribution of substation earthing resistance and nonuniqueness of the conductivity model to the final GIC uncertainties.
Dynamic Adaptive Environmental Flows (DAE‐Flows) to Reconcile Long‐Term Ecosystem Demands With Hydropower Objectives
Authors: Dalcin Ana Paula , Marques Guilherme Fernandes , Tilmant Amaury , Olivares Marcelo
Published: June 2023

This study investigates how environmental flows (e‐flows) can be designed as dynamic operating policies to optimize long‐term economic and ecosystem performance in reservoir systems. The main goal is to provide e‐flow solutions that contribute to better preparedness and flexibility of hydro‐systems to face multiyear stress periods, reducing the impact of water crises. The methodology framework combines a fish‐flow model with a multi‐objective evolutionary algorithm to construct multiple environmental water demand curves and capture the opportunity cost of different levels of ecosystem preservation. The water demand curves applied to a stochastic dynamic hydro‐economic model then derive dynamic e‐flow policies that balance immediate and future water use tradeoffs. The approach, termed dynamically adaptive environmental flows (DAE‐flows), is demonstrated on the Paraná River Basin, Brazil, a large‐scale hydropower system. Results show that the approach can adjust e‐flows (coordinated with other hydro‐system releases) over the time horizon, sacrificing them at certain times at the expense of some ecosystem loss, but improving long‐term ecosystem functioning. A long‐term approach to adaptation also yields better results for the environment without imposing a hard constraint to hydropower during droughts. Even under a drier climate change scenario, this allowed maintenance and improvement of environmental performance in most years, so during severe droughts the water could still be reallocated to hydropower but at a lesser cost to the environment.
Neutrophil extracellular traps aggravate neuronal endoplasmic reticulum stress and apoptosis via TLR9 after traumatic brain injury
Authors: Mi Liang , Min Xiaobin , Shi Mingming , liu Liang , Zhang Yanfeng , Zhu Yanlin , Li Peng , Chai Yan , Chen Fanglian , Deng Quanjun , Zhang Shu , Zhang Jianning , Chen Xin
Published: June 2023

Endoplasmic reticulum (ER) stress and ER stress-mediated apoptosis play an important role during secondary brain damage after traumatic brain injury (TBI). Increased neutrophil extracellular traps (NETs) formation has been demonstrated to be associated with neurological damage after TBI. However, the correlation between ER stress and NETs remains unclear, and the specific function of NETs in neurons has not been defined. In this study, we found that the levels of NETs circulating biomarkers were remarkably elevated in the plasma of TBI patients. We then inhibited NETs formation by peptidylarginine deiminase 4 (PAD4, a critical enzyme for NETs formation) deficiency and discovered that ER stress activation and ER stress-mediated neuronal apoptosis were reduced. The degradation of NETs via DNase I showed similar outcomes. Furthermore, overexpression of PAD4 aggravated neuronal ER stress and ER stress-associated apoptosis, while TLR9 antagonist administration abrogated the damage caused by NETs. In addition to in vivo experiments, in vitro experiments revealed that treatment with a TLR9 antagonist alleviated NETs-induced ER stress and apoptosis in HT22 cells. Collectively, our results indicated that ER stress as well as the accompanying neuronal apoptosis can be ameliorated by disruption of NETs and that suppression of the TLR9-ER stress signaling pathway may contribute to positive outcomes after TBI.
Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKKβ/NF-κB signaling
Authors: Ding Yan , Xu Xiaoli , Meng Biying , Wang Li , Zhu Biao , Guo Bei , Zhang Jiajia , Xiang Lin , Dong Jing , Liu Min , Xiang Guangda
Published: June 2023

Whether bone marrow modulates systemic metabolism remains unknown. Our recent study suggested that myeloid-derived growth factor (MYDGF) improves insulin resistance. Here, we found that myeloid cell-specific MYDGF deficiency aggravated hepatic inflammation, lipogenesis, and steatosis, and show that myeloid cell-derived MYDGF restoration alleviated hepatic inflammation, lipogenesis, and steatosis. Additionally, recombinant MYDGF attenuated inflammation, lipogenesis, and fat deposition in primary mouse hepatocytes (PMHs). Importantly, inhibitor kappa B kinase beta/nuclear factor-kappa B (IKKβ/NF-κB) signaling is involved in protection of MYDGF on non-alcoholic fatty liver disease (NAFLD). These data revealed that myeloid cell-derived MYDGF alleviates NAFLD and inflammation in a manner involving IKKβ/NF-κB signaling, and serves as a factor involved in the crosstalk between the liver and bone marrow that regulates liver fat metabolism. Bone marrow functions as an endocrine organ and serves as a potential therapeutic target for metabolic disorders.
Elevated levels of FMRP-target MAP1B impair human and mouse neuronal development and mouse social behaviors via autophagy pathway
Authors: Guo Yu , Shen Minjie , Dong Qiping , Méndez-Albelo Natasha M. , Huang Sabrina X. , Sirois Carissa L. , Le Jonathan , Li Meng , Jarzembowski Ezra D. , Schoeller Keegan A. , Stockton Michael E. , Horner Vanessa L. , Sousa André M. M. , Gao Yu ,   Birth Defects Research Laboratory, Glass Ian A. , Doherty Dan , Levine Jon E. , Wang Daifeng , Chang Qiang , Zhao Xinyu
Published: June 2023

Fragile X messenger ribonucleoprotein 1 protein (FMRP) binds many mRNA targets in the brain. The contribution of these targets to fragile X syndrome (FXS) and related autism spectrum disorder (ASD) remains unclear. Here, we show that FMRP deficiency leads to elevated microtubule-associated protein 1B (MAP1B) in developing human and non-human primate cortical neurons. Targeted MAP1B gene activation in healthy human neurons or MAP1B gene triplication in ASD patient-derived neurons inhibit morphological and physiological maturation. Activation of Map1b in adult male mouse prefrontal cortex excitatory neurons impairs social behaviors. We show that elevated MAP1B sequesters components of autophagy and reduces autophagosome formation. Both MAP1B knockdown and autophagy activation rescue deficits of both ASD and FXS patients’ neurons and FMRP-deficient neurons in ex vivo human brain tissue. Our study demonstrates conserved FMRP regulation of MAP1B in primate neurons and establishes a causal link between MAP1B elevation and deficits of FXS and ASD.
Campylobacter vaccination reduces diarrheal disease and infant growth stunting among rhesus macaques
Authors: Hendrickson Sara M. , Thomas Archana , Raué Hans-Peter , Prongay Kamm , Haertel Andrew J. , Rhoades Nicholas S. , Slifka Jacob F. , Gao Lina , Quintel Benjamin K. , Amanna Ian J. , Messaoudi Ilhem , Slifka Mark K.
Published: June 2023

Campylobacter-associated enteric disease is estimated to be responsible for more than 160 million cases of gastroenteritis each year and is linked to growth stunting of infants living under conditions of poor sanitation and hygiene. Here, we examine naturally occurring Campylobacter-associated diarrhea among rhesus macaques as a model to determine if vaccination could reduce severe diarrheal disease and infant growth stunting. Compared to unvaccinated controls, there are no Campylobacter diarrhea-associated deaths observed among vaccinated infant macaques and all-cause diarrhea-associated infant mortality is decreased by 76% (P = 0.03). By 9 months of age, there is a 1.3 cm increase in dorsal length that equaled a significant 1.28 LAZ (Length-for-Age Z score) improvement in linear growth among vaccinated infants compared to their unvaccinated counterparts (P = 0.001). In this work, we show that Campylobacter vaccination not only reduces diarrheal disease but also potentially serves as an effective intervention that improves infant growth trajectories.
Enhancing rice production sustainability and resilience via reactivating small water bodies for irrigation and drainage
Authors: Li Sisi , Zhuang Yanhua , Liu Hongbin , Wang Zhen , Zhang Fulin , Lv Mingquan , Zhai Limei , Fan Xianpeng , Niu Shiwei , Chen Jingrui , Xu Changxu , Wang Na , Ruan Shuhe , Shen Wangzheng , Mi Menghan , Wu Shengjun , Du Yun , Zhang Liang
Published: June 2023

Rice farming threatens freshwater resources, while also being increasingly vulnerable to drought due to climate change. Rice farming needs to become more sustainable and resilient to climate change by improving irrigation drainage systems. Small water bodies, used to store drainage water and supply irrigation in traditional rice farming systems have gradually been abandoned in recent decades. This has resulted in a higher water footprint (WF) associated with rice farming due to increased freshwater usage and wastewater release, also leaving rice production more vulnerable to extreme weather events. Here, we propose how protecting and reactivating small water bodies for rice irrigation and drainage can decrease rice production WF in China by 30%, save 9% of China’s freshwater consumption, increase irrigation self-sufficiency from 3% to 31%, and alleviate yield loss in dry years by 2–3%. These findings show that redesigning rice irrigation drainage systems can help meet water scarcity challenges posed by climate change.
An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells
Authors: Zhou Wenping , Li Wenxue , Wang Shisheng , Salovska Barbora , Hu Zhenyi , Tao Bo , Di Yi , Punyamurtula Ujwal , Turk Benjamin E. , Sessa William C. , Liu Yansheng
Published: June 2023

The serine/threonine kinase AKT is a central node in cell signaling. While aberrant AKT activation underlies the development of a variety of human diseases, how different patterns of AKT-dependent phosphorylation dictate downstream signaling and phenotypic outcomes remains largely enigmatic. Herein, we perform a systems-level analysis that integrates methodological advances in optogenetics, mass spectrometry-based phosphoproteomics, and bioinformatics to elucidate how different intensity, duration, and pattern of Akt1 stimulation lead to distinct temporal phosphorylation profiles in vascular endothelial cells. Through the analysis of ~35,000 phosphorylation sites across multiple conditions precisely controlled by light stimulation, we identify a series of signaling circuits activated downstream of Akt1 and interrogate how Akt1 signaling integrates with growth factor signaling in endothelial cells. Furthermore, our results categorize kinase substrates that are preferably activated by oscillating, transient, and sustained Akt1 signals. We validate a list of phosphorylation sites that covaried with Akt1 phosphorylation across experimental conditions as potential Akt1 substrates. Our resulting dataset provides a rich resource for future studies on AKT signaling and dynamics.
Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity
Authors: Rowe James , Grangé-Guermente Mathieu , Exposito-Rodriguez Marino , Wimalasekera Rinukshi , Lenz Martin O. , Shetty Kartika N. , Cutler Sean R. , Jones Alexander M.
Published: June 2023

The plant hormone abscisic acid (ABA) accumulates under abiotic stress to recast water relations and development. To overcome a lack of high-resolution sensitive reporters, we developed ABACUS2s—next-generation Förster resonance energy transfer (FRET) biosensors for ABA with high affinity, signal-to-noise ratio and orthogonality—that reveal endogenous ABA patterns in Arabidopsis thaliana. We mapped stress-induced ABA dynamics in high resolution to reveal the cellular basis for local and systemic ABA functions. At reduced foliar humidity, root cells accumulated ABA in the elongation zone, the site of phloem-transported ABA unloading. Phloem ABA and root ABA signalling were both essential to maintain root growth at low humidity. ABA coordinates a root response to foliar stresses, enabling plants to maintain foraging of deeper soil for water uptake.
Experience exceeds awareness of anthropogenic climate change in Greenland
Authors: Minor Kelton , Jensen Manumina Lund , Hamilton Lawrence , Bendixen Mette , Lassen David Dreyer , Rosing Minik T.
Published: June 2023

Although Greenland is a hub for climate science, the climate perceptions of Greenland’s predominantly Indigenous population have remained largely unstudied. Here we present two nationally representative surveys and show that Greenlanders are more likely than residents of top oil-producing Arctic countries to perceive that climate change is happening and about twice as likely to have personally experienced its effects. However, half are unaware that climate change is human-caused and those who are most affected appear to be least aware. Personal experience and awareness of human-induced climate change diverge along an Inuit cultural dimension. Indigenous identity positively predicts climate change experience, whereas subsistence occupation and no post-primary education negatively predict attribution beliefs. Despite Greenland’s centrality to climate research, we uncover a gap between the scientific consensus and Kalaallit views of climate change, particularly among youth. This science–society gulf has implications for local climate adaptation, science communication and knowledge exchange between generations, institutions and communities.
Response of stratospheric water vapour to warming constrained by satellite observations
Authors: Nowack Peer , Ceppi Paulo , Davis Sean M. , Chiodo Gabriel , Ball Will , Diallo Mohamadou A. , Hassler Birgit , Jia Yue , Keeble James , Joshi Manoj
Published: June 2023

Future increases in stratospheric water vapour risk amplifying climate change and slowing down the recovery of the ozone layer. However, state-of-the-art climate models strongly disagree on the magnitude of these increases under global warming. Uncertainty primarily arises from the complex processes leading to dehydration of air during its tropical ascent into the stratosphere. Here we derive an observational constraint on this longstanding uncertainty. We use a statistical-learning approach to infer historical co-variations between the atmospheric temperature structure and tropical lower stratospheric water vapour concentrations. For climate models, we demonstrate that these historically constrained relationships are highly predictive of the water vapour response to increased atmospheric carbon dioxide. We obtain an observationally constrained range for stratospheric water vapour changes per degree of global warming of 0.31 ± 0.39 ppmv K−1. Across 61 climate models, we find that a large fraction of future model projections are inconsistent with observational evidence. In particular, frequently projected strong increases (>1 ppmv K−1) are highly unlikely. Our constraint represents a 50% decrease in the 95th percentile of the climate model uncertainty distribution, which has implications for surface warming, ozone recovery and the tropospheric circulation response under climate change.
Exciton-assisted electron tunnelling in van der Waals heterostructures
Authors: Wang Lujun , Papadopoulos Sotirios , Iyikanat Fadil , Zhang Jian , Huang Jing , Taniguchi Takashi , Watanabe Kenji , Calame Michel , Perrin Mickael L. , García de Abajo F. Javier , Novotny Lukas
Published: June 2023

The control of elastic and inelastic electron tunnelling relies on materials with well-defined interfaces. Two-dimensional van der Waals materials are an excellent platform for such studies. Signatures of acoustic phonons and defect states have been observed in current-to-voltage measurements. These features can be explained by direct electron–phonon or electron–defect interactions. Here we use a tunnelling process that involves excitons in transition metal dichalcogenides (TMDs). We study tunnel junctions consisting of graphene and gold electrodes separated by hexagonal boron nitride with an adjacent TMD monolayer and observe prominent resonant features in current-to-voltage measurements appearing at bias voltages that correspond to TMD exciton energies. By placing the TMD outside of the tunnelling pathway, we demonstrate that this tunnelling process does not require any charge injection into the TMD. The appearance of such optical modes in electrical transport introduces additional functionality towards van der Waals material–based optoelectronic devices.
Different intrauterine environments and children motor development in the first 6 months of life: a prospective longitudinal cohort
Authors: Costa Wiltgen Andressa , Valentini Nadia Cristina , Beltram Marcelino Thiago , Santos Pinto Guimarães Luciano , Homrich Da Silva Clécio , Rombaldi Bernardi Juliana , Zubaran Goldani Marcelo
Published: June 2023

This prospective cohort longitudinal study examines the risk factors associated with different intrauterine environments and the influence of different intrauterine environments on children’s motor development at 3- and 6-months of life. Participants were 346 mother/newborn dyads enrolled in the first 24 to 48 h after delivery in public hospitals. Four groups with no concurrent condition composed the sample: mothers with a clinical diagnosis of diabetes, mothers with newborns small for gestational age due to idiopathic intrauterine growth restriction (IUGR), mothers who smoked tobacco during gestation, and a control group composed of mothers without clinical condition. Children were assessed at three- and six-months regarding motor development, weight, length, head circumference, and parents completed a socioeconomic questionnaire. The IUGR children had lower supine, sitting, and overall gross motor scores at 6 months than the other children’s groups. Anthropometric and sociodemographic characteristics negatively influenced gross motor development. IUGR and anthropometric and sociodemographic characteristics negatively impact motor development. Intrauterine environment impact child neurodevelopment.
Synthesis of magnetic electroactive nanomotors based on sodium alginate/chitosan and investigation the influence of the external electric field on the mechanism of locomotion
Authors: Mafakheri Fariba , Asakereh Ali , Khoee Sepideh , Kamankesh Mojtaba
Published: June 2023

In this paper, we report a novel electric-driven Janus nanomotor (JNMs) based on SPIONs nanoparticle decorated with chitosan (Cs) and sodium alginate (Na/Alg) using the Pickering emulsion method. The JNMs dispersed in aqueous media exhibit linear trajectories under DC electric field, and the driving force is attributed to the self-electro-osmotic mechanism and surface modifications. This study offers an approach to remotely control the motion modes of the JNMs, including start, stop, directional and programmable motion, which can be advantageous for various application scenarios. The diffusion coefficient and velocity of the JNMs were investigated through mean square displacement analysis for single particle of JNMs, both in distilled water and in the presence of different di and trivalent metal cations (Fe3+, Al3+, Ba2+, Ca2+ and Mg2+) as crosslinking agents, as well as monovalent salts (LiCl and KCl). The results revealed that the motion of JNMs was fastest in the presence of Fe3+ as crosslinker agent (about 7.2181 μm2/s) due to its higher charge than equimolar Na+ . Moreover, it was demonstrated that increasing the ionic strength led to relatively higher speeds of JNMs, as the solution polarity increased and, as a result, the driving force of electro-osmoesis enhanced.
Sensemaking for entangled urban social, ecological, and technological systems in the Anthropocene
Authors: Chester Mikhail V. , Miller Thaddeus R. , Muñoz-Erickson Tischa A. , Helmrich Alysha M. , Iwaniec David M. , McPhearson Timon , Cook Elizabeth M. , Grimm Nancy B. , Markolf Samuel A.
Published: June 2023

Our urban systems and their underlying sub-systems are designed to deliver only a narrow set of human-centered services, with little or no accounting or understanding of how actions undercut the resilience of social-ecological-technological systems (SETS). Embracing a SETS resilience perspective creates opportunities for novel approaches to adaptation and transformation in complex environments. We: i) frame urban systems through a perspective shift from control to entanglement, ii) position SETS thinking as novel sensemaking to create repertoires of responses commensurate with environmental complexity (i.e., requisite complexity), and iii) describe modes of SETS sensemaking for urban system structures and functions as basic tenets to build requisite complexity. SETS sensemaking is an undertaking to reflexively bring sustained adaptation, anticipatory futures, loose-fit design, and co-governance into organizational decision-making and to help reimagine institutional structures and processes as entangled SETS.
Seasonal forecasting of subsurface marine heatwaves
Authors: McAdam Ronan , Masina Simona , Gualdi Silvio
Published: June 2023

Marine heatwaves damage marine ecosystems and services, with effects identified mostly below the ocean surface. To create a truly user-relevant detection system, it is necessary to provide subsurface forecasts. Here, we demonstrate the feasibility of seasonal forecasting of subsurface marine heatwaves by using upper ocean heat content. We validate surface and subsurface events forecast by an operational dynamical seasonal forecasting system against satellite observations and an ocean reanalysis, respectively. We show that indicators of summer events (number of days, strongest intensity, and number of events) are predicted with greater skill than surface equivalents across much of the global ocean. We identify regions which do not display significant surface skill but could still benefit from accurate subsurface early warning tools (e.g., the mid-latitudes). The dynamical system used here outperforms a persistence model and is not widely influenced by warming trends, demonstrating the ability of the system to capture relevant subseasonal variability.