bluuebunny/crossref_metadata_2025 huggingface dataset:
İçsel ve Dışsal Motivasyon ile Görev Performansı Arasındaki İlişki: Tekstil Sektöründe Bir Araştırma
Authors: Irk Ersin , Bıyık Hasan
Published: November 2024

Arka plan. Motivasyon performans ilişkisi çok fazla araştırmaya konu edilse de konunun önemine binaen güncel bir araştırma konusu olmaya devam etmektedir. Bu ilişkiye özellikle mavi yakalı çalışanlar bağlamında ve Türkiye gibi gelişmekte olan üretim ülkeleri örnekleminde odaklanmanın önemli olduğu düşünülmektedir.
Amaç. Bu çalışmanın temel amacı tekstil çalışanlarının içsel ve dışsal motivasyonlarının görev performanslarına nasıl etki ettiğini tespit etmektir.
Yöntem. Araştırmanın ana kütlesini Tokat ili Erbaa ilçesinde görev yapan tekstil fabrikalarının mavi yakalı çalışanları oluşturmaktadır. Saha anket çalışması sonucu 166 adet ve 62 adet çevrimiçi anketle birlikte toplam 228 adet anket verisi elde edilmiştir. Katılımcılara uygulanan ankette demografik özellikler ile motivasyon ve görev performansı ölçeklerine ait sorulara yer verilmiş olup verilerin analizinde SPSS ve AMOS istatistik paket programları kullanılmıştır.
Bulgular. Araştırma sonucunda içsel ve dışsal motivasyonun görev performansının %48'ini açıkladığını ortaya konmaktadır. Görev performansı üzerinde hem içsel hem de dışsal motivasyonun pozitif etkisi belirlenmiştir. İçsel motivasyonun dışsal motivasyona göre daha fazla olumlu etki oluşturduğu tespit edilmiştir.
Sonuç.  Bu araştırma literatürde mevcut motivasyon performans ilişkisini doğrulamaktadır. Araştırmanın özellikle mavi yaka çalışanlar ile yapılması ve Türkiye örnekleminde emek yoğun bir sektörde gerçekleştirilmesi araştırmanın önemini arttırmaktadır.
Is Conformist Behavior Related to Boreout?
Authors: Gökçen-Kapusuz Ayşe , Şalvarcı Ümit
Published: November 2024

Background. The main reason for conducting such a study is to find the answer to the question of whether the boredom they experience at work is behind these behaviors in business life, where individuals push themselves into the background rather than using their right to speak. In this context, understanding the concept of conformism, which is new in the literature, and analyzing its relationships with relevant variables will contribute to the literature.
Aim. The aim of this study is to investigate whether conformist behaviors that individuals frequently encounter and are exposed to in business life are related to boreout experienced for some reasons.
Method. In this context, through a relational model designed according to the quantitative research method, the research data obtained from the teachers' sample (n = 325) were subjected to statistical analyses and the findings were reported.
Findings. The independent variable boreout and its all-sub-dimensions have a significant and positive contribution to explaining conformist behaviors. 60% of the variance in conformist behaviors can be explained by boredom/boreout at work. Individuals exhibit more conformist behavior by questioning their work, development and meaning due to the boredom they experience.
Conclusion. The increase in boreout level of employees, which arises for some reasons in the work environment, will be effective in employees' ability to express their opinions, express their thoughts, and decrease their participation in decisions, in other words, to increase their conformist behaviors. Because individuals want to be comfortable in the work environment where they spend most of their days and not to encounter factors that will cause boredom in order to be able to express themselves and put forward an idea on a subject.
Platform Pragmatics
Authors: Biscossi Edoardo
Published: November 2024

This article proposes platform pragmatics as a framework for understanding collective behaviour and forms of labour within platform ecosystems. It contributes to the field of platform criticism by problematising a certain view of users as passive victims of surveillance and algorithmic governmentality. The main argument is developed by thinking through the production of content and forms by users, and their circulation through computational logic and aective contagion. Through some illustrative cases and analyses of cultural habits, the article addresses the political and aesthetic configuration of these forms of production — not only of content/forms, but also of culture and subjectivity. This is explored by thinking through three themes: the subsumption of creativity and opportunism in platform economies; the mobilisation of speculative temporalities not only in computation but also across user practices; and the generalisation of self-reflexivity as a feminised cultural behaviour and aesthetic mode. Finally, I propose to understand platform pragmatics as a mode of subaltern power, that might be alien to traditional political reason, but precisely because of this needs to be grappled with through inventive cultural and social criticism.
Une revue intégrative répertoriant la créativité dans l’utilisation de la santé numérique et de l’intelligence artificielle à l’Institut universitaire en santé mentale de Montréal
Authors: Hudon Alexandre , Beaudoin Mélissa
Published: November 2024

Objectif Fondé en 1873, l’Institut universitaire en santé mentale de Montréal (IUSMM) possède une histoire captivante qui reflète de manière significative l’évolution de la psychiatrie au fil des ans. Au courant des dernières années, les principaux secteurs de la médecine moderne se sont orientés vers les innovations en santé numériques pour améliorer le développement des soins aux patients. L’objectif principal de cette revue est de répertorier les avancées dans le domaine de la santé numérique élaborées au sein de l’IUSMM et de son centre de recherche affilié au cours des 150 dernières années.

Méthode Une revue intégrative portant sur les innovations en santé numérique au sein de l’IUSMM a été réalisée. Les bases de données Medline, Web of Science, PsycNet (PsycINFO) et Google Scholar ont été consultées depuis leur création jusqu’à décembre 2023.

Résultats La revue de la littérature a initialement identifié 239 articles, dont 108 doublons ont été éliminés lors de l’analyse préliminaire. Parmi les 131 études restantes, 63 articles ont été exclus après examen des titres et résumés, car ils ne répondaient pas aux critères d’inclusion. Après une analyse complète des 68 articles initialement sélectionnés pour évaluation d’éligibilité, un total de 22 articles a été retenu. Cinq catégories d’articles ont été identifiées : la réalité, les applications numériques, les prestations des services et thérapies à distance, l’utilisation de l’intelligence artificielle et finalement l’assistant personnel digital.

Conclusion À l’occasion du 150e anniversaire de l’IUSMM, cette revue intégrative révèle que les innovations en santé numérique sont concentrées principalement dans la dernière décennie, suggérant un potentiel prometteur pour des applications numériques dans le soutien quotidien aux personnes souffrant de troubles mentaux, bien que la transition à la pratique clinique puisse progresser lentement.
Le bien-être numérique, un enjeu de santé mentale technologique : la place du téléphone intelligent
Authors: Khazaal Yasser , Vera Cruz Germano
Published: November 2024

Objectifs Présenter le concept de bien-être numérique et explorer la place d’outils digitaux au service de ce bien-être.

Méthode L’article se base sur une description du concept de bien-être numérique, des modèles d’intervention actuellement disponibles et des possibilités d’évolution de ces modèles.

Résultats L’utilisation des téléphones intelligents ou « smartphones » est devenue omniprésente dans la société moderne, en particulier chez les adolescents et les jeunes adultes. Avec une connectivité intelligente, interactive, pratique et permanente, les individus sont confrontés à de nouveaux défis et à de nouvelles pressions liées au moment, à l’endroit, à la manière et à l’intensité avec lesquels ils décident de se connecter ou de se déconnecter des services fournis par le téléphone intelligent. L’utilisation problématique des téléphones intelligents a été associée à des troubles de la santé mentale et à des problèmes fonctionnels. Face à cet enjeu d’un usage du numérique au service du bien-être, le concept de bien-être numérique a récemment été introduit. Il s’agit de l’expérience subjective et individuelle d’un équilibre optimal entre les avantages et les inconvénients associés à la technologie numérique. Des outils technologiques orientés sur le contrôle des temps d’usage des écrans sont proposés. Les résultats de ces approches sont mitigés.

Conclusion Pour une efficacité optimale, une meilleure intégration des interfaces dynamiques entre l’humain et la machine pourrait être essentielle.
Développer le flair du psychiatre : des enseignements tirés de deux cas cliniques de syndrome de référence olfactive
Authors: Masse Morganne , Stip Emmanuel
Published: November 2024

L’olfaction est souvent négligée en sémiologie psychiatrique, malgré sa pertinence clinique et son impact sur le fonctionnement social. Le syndrome de référence olfactive (SRO), qui se manifeste par une fausse croyance selon laquelle une personne émet une odeur nauséabonde, entraîne une détresse sévère et altère le fonctionnement social. Cet article cherche à souligner la pertinence de l’olfaction dans la sémiologie psychiatrique, en mettant l’accent sur le SRO. Nous présentons une étude de deux hommes atteints de SRO, suivis en unité de troubles psychotiques, selon les critères CARE. Une revue de littérature effectuée selon les critères PRISMA, examine 53 études issues de PubMed, PsychInfo, Google Scholar et Cairns et complète les présentations cliniques.

Cette analyse permet ainsi une discussion sur les connaissances actuelles du SRO, en réfutant, nuançant et validant les hypothèses en vigueur sur la nosographie et l’étiologie du trouble. Nous proposons d’approfondir le rôle de l’olfaction, en explorant son association avec les émotions, la formation des impressions et la Théorie de l’Esprit. Affiner notre compréhension de l’influence de l’olfaction sur le fonctionnement psychique et social pourra enrichir nos approches diagnostiques et thérapeutiques en psychiatrie du SRO.
Un regard sur le domaine de la psychothérapie et sur le développement du programme APAP
Authors: Borgeat François , Amado Danielle , Lallier Carmen , Barabé Joanie
Published: November 2024

Objectif Le domaine des psychothérapies est en demande accrue et ses offres s’avèrent très diversifiées. L’objectif du présent article est d’effectuer un survol basé sur des recherches cliniques et sur des résultats de psychothérapeutes avec des formes de psychothérapies établies et pratiquées dans des milieux universitaires et plus récemment à l’Institut universitaire en santé mentale de Montréal.

Méthode Les méthodes de recherche et d’évaluations cliniques ont changé au cours de plusieurs années. Les psychothérapeutes ont varié leurs approches selon les milieux et plusieurs ont participé à de nombreuses recherches et publications. Les recherches au fil des années sont discutées ce qui inclut le développement de stratégies récentes dont le programme informatisé APAP ainsi appelé pour Augmentation de la Psychothérapie par Amorçage Préconscient. Cette méthode vise à faciliter le changement cognitif et propose une stratégie d’écoute préconsciente répétitive.

Résultats Les recherches s’étendent sur plusieurs années, car les psychothérapeutes étaient aussi des cliniciens et leurs contributions variaient. Par exemple, certains premiers travaux avec des patients volontaires montraient l’intérêt des écoutes préconscientes répétitives. Puis, par la suite, dans le contexte de l’IUSMM, une recherche à double insu a été réalisée et a montré l’efficacité de ce nous avons appelé l’amorçage préconscient pour faciliter le changement cognitif.

Conclusion La recherche sur la psychothérapie demeure difficile et s’étale sur de longues périodes, par exemple la recherche mentionnée plus haut s’est étendue sur plus d’une année. Les recherches à double insu s’avèrent peu nombreuses, car les placebos crédibles sont rares. Notre recherche était favorisée par l’écoute préconsciente échappant à la conscience, et il nous était possible de comparer les effets de stimuli porteurs de messages préconscients visant les besoins des patients à divers contenus variés ou neutres, par exemple des nombres aléatoires.
Balancing microbial composition through diet
Authors: Acuff L Catalina , Guillemin Karen
Published: November 2024

A reciprocal interaction between gut bacteria and gut cells affects protein absorption in the host.
Análise de custos para fabricação de cases plásticos por manufatura aditiva
Authors: Duarte Filho Claudio , Oliveira Jozias Parente de , Furtado Diogo Abreu , Cisneros Edry Antonio Garcia , Gomes Raimundo Cláudio Souza , Printes André Luiz , Sobrinho Angilberto Muniz Ferreira , Teixeira Renan Almeida
Published: November 2024

A indústria de eletrônicos é um dos setores mais competitivos do mercado, onde o tempo de introdução de um produto (time-to-market) é crucial, assim como a redução do tempo de fabricação. Isso exige abordagens eficientes de prototipagem e manufatura. Assim, a Manufatura Aditiva (MA) se destaca como um método eficaz para o desenvolvimento de compartimentos plásticos, permitindo a produção rápida e precisa de protótipos e produtos finais. Neste artigo apresenta uma análise comparativa dos custos de fabricação de cases para placas eletrônicas utilizando os métodos de injeção plástica e impressão 3D. Para um lote de 6000 unidades, foram coletados orçamentos que revelaram que os custos associados à injeção plástica são consistentemente menores em comparação com a impressão 3D. Os custos de fabricação foram separados em custos de moldes e custos por peça, permitindo uma avaliação de- talhada das despesas de cada fornecedor. A análise gráfica mostrou que, apesar dos altos custos iniciais dos moldes na injeção plástica, o custo por unidade diminui significativamente com o aumento do volume de produção, tornando este método mais econômico para grandes quantidades. Em contraste, a impressão 3D, embora tenha menores custos iniciais e ofereça maior flexibilidade, apresenta custos mais elevados por peça em grandes volumes. O estudo conclui que a injeção plástica é a opção mais viável economicamente para produção em larga escala, enquanto a impressão 3D é mais indicada para produções menores ou para peças com designs complexos e personalizações frequentes.
Actualización del conocimiento de los sistemas coralinos poco conocidos del sur del Pacífico mexicano
Authors: López-Pérez Andrés , Granja-Fernández Rebeca , Valencia-Méndez Omar , González-Mendoza Tania , Ramírez-Chávez Eduardo , Pañola-Madrigal Abigail , López-López Daniel , Calderón-Aguilera Luis E. , Rodríguez-Zaragoza Fabián A.
Published: November 2024

Puerto Ángel (PA), Puerto Escondido (PE) y Punta Maldonado (PM) albergan ecosistemas coralinos en el sur del Pacífico mexicano (SPM); sin embargo, estos ecosistemas se encuentran dentro de los menos evaluados y prospectados. Este trabajo proporciona un inventario de especies de coral y contribuye a la caracterización de estos sistemas para el SPM. Entre 2009 y 2023 se prospectaron 15 sitios (PA = 9; PE = 4; PM = 2) mediante buceo errante y transectos de punto intercepto realizados por buzas y buzos con equipo de buceo autónomo, así como vehículos submarinos operados remotamente. Se registraron un total de 10 especies de corales (PA = 10; PE = 5; PM = 2) de los géneros Pocillopora (aguas someras; <30 m de profundidad), Pavona y Porites (<37 m de profundidad). Por primera vez en PM, se registraron corales distribuidos en aguas profundas (<37 m de profundidad) y alejadas de la línea de costa, lo cual es poco común en los sistemas de coral del SPM. Esto puede deberse al límite más amplio de la zona mesofótica y a la anómala y extensa plataforma continental de PM formada por procesos geológicos. Puerto Ángel presentó una mayor cobertura coralina (30.2% ± 21.9) en comparación con la de PE (6.6% ± 8.7), donde predominaba el sustrato rocoso. Estos porcentajes son menores que los reportados para otras regiones del SPM y son consecuencia de las características geomorfológicas de las áreas, pero principalmente se deben a las perturbaciones antropogénicas que han experimentado a lo largo del tiempo, como el cambio en el uso de suelo y la extracción de colonias de coral para los mercados de artesanías.
Self-Awareness Learning in Relation to Educational Development of Students in Public Elementary Schools
Authors: Derla Honey Mae C. , Baguio Josephine B.
Published: November 2024

This study examined the relationship between self-awareness learning and the educational development of students in public elementary schools in the Panabo District, Division of Davao Del Norte. A non-experimental quantitative research design with a correlational method was employed, with 133 student respondents selected through universal sampling. Data analysis included the use of mean, Pearson's r, and regression analysis. Findings indicated that students sometimes manifested self-awareness, and their educational development was similarly moderate. Results showed a significant relationship between self-awareness learning and students' educational development, with specific domains of self-awareness significantly influencing educational outcomes. These findings suggest that enhancing self-awareness can be a valuable tool in educational practices. Teachers may benefit from attending seminars and professional development programs offered by DepEd to strengthen their instructional strategies. By building teaching capacity, educators can foster an environment that promotes students' self-awareness and supports comprehensive educational development.
Instructional Practices and Learning Metaphors of Language Teachers in Public Secondary Schools
Authors: Pasculado Pearl Anne G. , Baguio Josephine B.
Published: November 2024

This study was conducted to determine the instructional practices and learning metaphors of teachers in Caraga District, Division of Davao Oriental. This study employed the non-experimental quantitative research design utilizing correlational method. The respondents of the study were the 131 teachers utilizing the universal sampling in selecting the actual respondents. The following were the statistical tools used in the computation of data these are the Mean. Product Moment Coefficient Correlation or Pearson r and Regression Analysis. Based on the findings obtained in this study, the following conclusions were drawn, the level of instructional practices of teachers were extensive in the areas of mastery of the subject matter, appropriate use of methods, communication skills, evaluation and feedback. On the other hand, the learning metaphors of teachers likewise were very extensive in the areas of teachers of reflection of effective teaching, clarity of expectations, guided performance, experimentation and discovery. There was a significant relationship between instructional practices and learning metaphors of teachers in public elementary schools. It is recommended that the DepEd officials should support the development in instructional practices of teachers. This is to increase the level of their holistic knowledge in the areas of mastery of the subject matter, appropriate use of methods, communication skills, evaluation and feedback in augmenting school academic and non-academic operations.
Study of Nitrogen Use Efficiency in Potato (Solanum tuberosum L.)
Authors: Sandilya Vivek Kumar , Rangare N. R. , Khokhar Dharmendra , Kumar Vikky , Rangare Smita Bala
Published: November 2024

The study was conducted under All India Coordinated Research project on potato at Research cum Instructional farm, Department of Genetics and Plant Breeding, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur, (C.G.) during Rabi season 2021-22, in split plot design with three replications, for this research the experimental material consisting of twenty potato genotypes including two check variety Kufri Khyati and Kufri Chipsona-1.With three Nitrogen levels (0, 180 and 250 kg N/ ha), to find out the N requirement and Nitrogen use efficiency of Potato genotypes. The results of the present experiment revealed that all the genotypes are showing genotypic variability for NUE, there is sufficient variation for NUE. The genotypes used in the experiment show response to nitrogen and performing different the same genotype in three different nitrogen levels, among the twenty genotypes P-73 recorded maximum Nitrogen Use efficiency at all the levels of nitrogen followed by Kufri Mohan and Kufri Lalima. Genotype P-73 proved to possess high tolerance to N stress and was the most nitrogen-efficient variety followed by Kufri Mohan and Kufri Neelkanth. This experiment can be concluded that, the variety responded the same at 180 and 250 Kg Nitrogen can be recommended to apply only 180 kg of Nitrogen, the knowledge of NUE can save the money and land of farmers by minimal use of Nitrogen.
Screening of Mango Varieties against Anthracnose Diseases Caused by Colletotrichum gloeosporioides and Its In vitro Management through Biocontrol Agents and Fungicides
Authors: Lamare Nikiru , N.V. Radhakrishnan , Thara Susha S. , S Simi , Kumar Athulya S , Rithesh Lellapalli
Published: November 2024

Mango (Mangifera indica L.), known as the "King of Fruits," is a major fruit crop cultivated in India and worldwide. Anthracnose, caused by Colletotrichum gloeosporioides, is a primary biotic stress affecting mango production, yield, and export quality in all mango-growing regions. This study evaluated the response of mango varieties to anthracnose and its management using effective fungicides and biocontrol agents. Ten C. gloeosporioides isolates were isolated from samples collected from the Kollam and Thiruvananthapuram districts in Kerala. Among them, isolate C10 from Thiruvananthapuram was the most virulent. Among five local mango varieties (Kottukonam, Priyoor, Neelam, Rumani, and Totapuri) screened through artificial inoculation, Totapuri displayed the lowest disease severity (45.4%), followed by Neelam and Rumani, while Kottukonam exhibited the highest (72.33%). In vitro, the study of different biocontrol agents showed that Bacillus amyloliquefaciens VLY24 showed 37.08% inhibition against the pathogen, followed by 32.87% inhibition by Bacillus velezensis PSCE-10. Carbendazim 50% WP at 0.1% and 0.2% exhibited 100% mycelial growth suppression.
Assessing Different Rice Genotypes Tolerance against the Major Pests and Identifying the Biochemical Basis of Tolerance
Authors: Nalla Anil Varma , Kumar K. , Nadaradjan S. , Adiroubane D.
Published: November 2024

A total of 196 rice genotypes were screened for tolerance against paddy stem borer and leaf folder pests under open field conditions. Damage assessment followed the standard evaluation procedure for rice developed by IRRI (1988). Stem borer incidence was recorded during both the vegetative and reproductive stages while leaf folder incidence was recorded at five different growth stages (30, 40, 50, 60, and 70 days after transplanting). Interestingly, tolerance to paddy stem borer varied among the genotypes. Genotypes resistant at the Vegetative Stage did not show tolerance at the Reproductive Stage. Consequently, leaf folder infestation was recorded at five different growth stages, demonstrating that although the mean infestation was considered for identifying tolerance, the rate of infestation against an accession is not consistent across all growth stages. Additionally, biochemical analysis of the resistant entries, along with a susceptible check (TN1), revealed that higher total phenol concentrations, moderate chlorophyll content, and lower sugar levels were key factors contributing to pest tolerance. The correlation between infestation percentage and biochemical parameters showed a high positive correlation between total sugars and infestation percentage and a strong negative correlation between total phenols and infestation percentage, indicating that phenols play a role in plant defense.
Foreign Direct Investment and Growth in ECOWAS: An ARDL Panel Analysis
Authors: J. Ajayi Kayode
Published: November 2024

This study examines the impact of core macroeconomic indicators on growth across Sixteen (16) West African countries using the Pooled Mean Group (PMG) estimation technique within a Panel ARDL framework. The analysis covers variables such as Foreign Direct Investment (FDI), Gross Capital Formation, percentage of working population growth, access to electricity, and inflation. The findings reveal that the effects of FDI on growth were mixed. While FDI positively impacts real GDP growth in some countries such as Benin, Cabo Verde, and Mauritania, its effect was however, negative in others such as Nigeria and Gambia. Gross capital formation, on the other hand, consistently supports short-run growth across most countries, while inflation emerges as a destabilizing factor. The speed of adjustment toward long-run equilibrium varies significantly, with countries such as Guinea-Bissau and Nigeria correcting economic shocks faster than others. This study contributes to the literature on economic growth dynamics in Africa by showing the heterogeneous responses of these countries to similar macroeconomic factors. It validates the need for country-specific policies that focus on stabilizing inflation, optimizing domestic investment, and effectively leveraging foreign direct investment. Also, improvements in infrastructure and access to markets are crucial for facilitating long-term growth across the region. The study provides valuable insights for policymakers and stakeholders aiming to achieve sustainable growth in the ECOWAS region.
Use Of Jute In Road Subgrade
Authors: Rahman MD Rafiur , Rofi MDRofiul Islam
Published: November 2024

In recent years jute geotextile used different areas of construction.It is relatively cheap and durable.It increases the bearing capability of soil that used in construction.It can be used in different purposes such as preventionof soil erosion,strengthening road subgrade etc.By using differentlength of jute fibre in pavement the strength of the subgrade can be increased. CBR value for different position of road has been studied by using different length of moulde of jute.In this review paper we studied about uses of jute to improve the strength of subgrade on road construction with the help of different kind of soil.Black Cotton soil,Murum soil and Mix soil are used to compare CBR value.
Improvements in treatment planning calculations motivated by tightening IMRT QA tolerances
Authors: Stambaugh Cassandra , Gagneur Justin , Uejo Arielle , Clouser Edward , Ezzell Gary
Published: January 2019

Implementing tighter intensity modulated radiation therapy (IMRT) quality assurance (QA) tolerances initially resulted in high numbers of marginal or failing QA results and motivated a number of improvements to our calculational processes. This work details those improvements and their effect on results. One hundred eighty IMRT plans analyzed previously were collected and new gamma criteria were applied and compared to the original results. The results were used to obtain an estimate for the number of plans that would require additional dose volume histogram (DVH)‐based analysis and therefore predicted workload increase. For 2 months and 133 plans, the established criteria were continued while the new criteria were applied and tracked in parallel. Because the number of marginal or failing plans far exceeded the predicted levels, a number of calculational elements were investigated: IMRT modeling parameters, calculation grid size, and couch top modeling. After improvements to these elements, the new criteria were clinically implemented and the frequency of passing, questionable, and failing plans measured for the subsequent 15 months and 674 plans. The retrospective analysis of selected IMRT QA results demonstrated that 75% of plans should pass, while 19% of IMRT QA plans would need DVH‐based analysis and an additional 6% would fail. However, after applying the tighter criteria for 2 months, the distribution of plans was significantly different from prediction with questionable or failing plans reaching 47%. After investigating and improving several elements of the IMRT calculation processes, the frequency of questionable plans was reduced to 11% and that of failing plans to less than 1%. Tighter IMRT QA tolerances revealed the need to improve several elements of our plan calculations. As a consequence, the accuracy of our plans have improved, and the frequency of finding marginal or failing IMRT QA results, remains within our practical ability to respond.
LncRNA MAFG‐AS1 facilitates the migration and invasion of NSCLC cell via sponging miR‐339‐5p from MMP15
Authors: Jia You Chao , Wang Jian Yi , Liu Yu Ying , Li Bin , Guo Hui , Zang Ai Min
Published: January 2019

Non‐small‐cell carcinoma (NSCLC) is the most common cancer along with high mortality rate worldwide. In the present study, our data showed that lncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAFG‐AS1) was over‐expressed in NSCLC tissues and cell lines. Overexpression of MAFG‐AS1 promoted the migration, invasion and enhanced epithelial‐mesenchymal transition (EMT) of NSCLC cell. In addition, miR‐339‐5p was predicted to be a target of MAFG‐AS1 and the level of miR‐339‐5p was down‐regulated in NSCLC. Over‐expression of MAFG‐AS1 significantly decreased the level of miR‐339‐5p in NSCLC cell. Moreover, the matrix metalloproteinase 15 (MMP15) was identified to be a target of miR‐339‐5p. The level of MMP15 was negatively regulated by miR‐339‐5p whereas positively controlled by MAFG‐AS1. In addition, up‐regulation of miR‐339‐5p neutralized the promoting impact of MAFG‐AS1 on the migration, invasion and EMT of NSCLC cell. Finally, the xenograft model suggested that MAFG‐AS1 promoted the metastasis of NSCLC cell in vivo. Altogether, we proved that MAFG‐AS1‐miR‐339‐5p‐MMP15 axis might be a promising therapeutic target for the treatment of patients with NSCLC.
Catalan Virgin Olive Oil Protected Designations of Origin: Physicochemical and Major Sensory Attributes
Authors: Vichi Stefania , Tres Alba , Quintanilla‐Casas Beatriz , Bustamante Julen , Guardiola Francesc , Martí Esteve , Hermoso Juan F. , Ninot Antonia , Romero Agustí
Published: January 2019

Catalonia, located in the northeast of Spain, comprises five extra virgin olive oil (EVOO) protected designations of origin (PDOs). Despite the proximity between them, these PDOs represent unique pedoclimatic conditions and traditional olive cultivars that are briefly reviewed in the present manuscript. In addition to the compliance with quality standards fixed by product specifications, EVOOs show singular and distinctive composition and sensory profiles. With the aim to describe the characteristics of Catalan EVOOs, their sensory and analytical traits are reviewed with the support of data collected between 2009 and 2017 in more than 42 milling facilities from the five Catalan PDOs, within the frame of official surveys launched by the Catalan Government.

Practical Applications: A detailed knowledge of the characteristics of differentiated‐quality productions will favor their valorization and protection, improving their image and increasing the consumer confidence. For this reason, studies to objectively define the characteristics of PDO EVOOs are useful tools to promote this sector.

Despite the proximity between Catalan PDOs, their unique pedoclimatic conditions and traditional olive cultivars contribute to produce extra virgin olive oils with singular and distinctive composition and sensory profiles.
Recent Amendment to Product Specification of Brisighella PDO (Emilia‐Romagna, Italy): Focus on Phenolic Compounds and Sensory Aspects
Authors: Barbieri Sara , Bendini Alessandra , Gallina Toschi Tullia
Published: January 2019

The Brisighella Protected Designation of Origin (PDO) extra virgin olive oil (EVOO) has been protected by European trademark since 1996. It is obtained from olives belonging to the cv “Nostrana di Brisighella” (must make up at least 90% of groves) grown exclusively in Brisighella, a circumscribed area of the Emilia‐Romagna region, in the north of Italy. Brisighella PDO EVOO is produced by the unique plant mill of the Consortium (Consorzio Olio DOP “Brisighella”). In this research, minor amendments to product specification of Brisighella PDO approved by the EU in 2016 are described. The proposed modifications to the Brisighella PDO product specification are supported by chemical‐sensory analyses carried out on 15 EVOOs from cultivars autochthonous of the Emilia‐Romagna region (Nostrana di Brisighella and Ghiacciolo), selected as representatives of 14 geographical sites (small farms) with different characteristics in terms of climatic and environmental conditions (e.g., altitude, geographical orientation and slope of the olive orchard) of the production area established in the product specification. These results are also compared with available data of EVOOs certified as PDO Brisighella in the period 2004–2012 and commissioned by the responsible certification control body.

Practical Application: The product specification represents the product's identification document that must contain a description of the requirements that the product must comply with (e.g., product description in terms of chemical and sensory characteristics, delimitation of geographical area and evidence of the link with the territory, methodology applied, etc.). It also must include the references relative to control systems; therefore, amendments according to the updating of EU legislation in the sector are desirable to assure the final consumer that the expected value of the product effectively corresponds with what has been declared. Moreover, considering the growing interest of consumers toward health properties, this update will contribute to communicating the intrinsic properties of olive oil and benefits to health, enhancing the product image and promoting informed consumption.

Recent amendment to product specification of Brisighella PDO (Emilia‐Romagna, Italy). Considering the growing interest of consumers toward health properties, this update will contribute to communicate the intrinsic properties of olive oil and benefits to health, enhancing the product image, and promoting informed consumption.
Optimized Analysis of MCPD‐ and Glycidyl Esters in Edible Oils and Fats Using Fast Alkaline Transesterification and13C‐Correction for Glycidol Overestimation: Validation Including Interlaboratory Comparison
Authors: Zwagerman Ralph , Overman Pierre
Published: January 2019

As of March 2018, legislation has come into force within the European Union (EU) that places maximum levels on the glycidyl ester (GE) content of vegetable oils intended for use in foodstuffs for the European market. As a result, producers of edible oils are obliged to closely monitor the GE levels in all their products. Unfortunately, the three available AOCS methods to measure GE are not really suited for application in a quality control (QC) laboratory within a production facility, where short turnaround times are vital. AOCS Cd29c‐13 has the highest potential from a turnaround perspective, but remains disputable because it is based on a differential GE measurement. Hence, in 2016 the authors published an automated procedure in which the shortcomings of the AOCS Cd29c‐13 are effectively addressed. This article presents the results of an interlaboratory comparison, which is organised across six laboratories in four countries. Methods involved include AOCS Cd29b‐13 as well as an optimized version of the 2016 method, which is carried out both automatically and manually. Prior to validation, several optimizations are implemented to further reduce turnaround time and improve robustness of the original method. Based on the results of the interlaboratory comparison, it is concluded that the automated procedure performs slightly better than the manual alternative. Compared to the AOCS Cd29b‐13, no significant differences are observed for various types and blends of vegetable oils.

Practical Application: Because the EU has recently enforced its legislation for maximum GE levels in edible oils, QC laboratories across the EU are looking for methods which are easily implemented. In practice, this implies high quality, low costs and high sample capacity. Food safety demands fast and reliable analysis in order to verify the quality of refined edible oils and fats. Due to the toxicological relevance of these process contaminants, turnaround time is a crucial performance parameter and any possibility of reducing analysis time has to be fully exploited. The method presented here, provides the short turnaround times required for these purposes and is validated by interlaboratory comparison. This is crucial in the coming years when new EU legislation as well as efforts by food companies will continue to push the continuation of mitigation efforts by edible oil producers.

Graphical overview of serial versus parallel preparation of seven authentic samples and the profound effect on the total sample preparation time required for the analysis of MCPDe and GE in edible oils.
microRNA‐27a and microRNA‐146aSNPin cerebral malaria
Authors: Wah Saw Thu , Hananantachai Hathairad , Patarapotikul Jintana , Ohashi Jun , Naka Izumi , Nuchnoi Pornlada
Published: January 2019

Background

DuringPlasmodium falciparuminfection, microRNAexpression alters in brain tissue of mice with cerebral malaria compared to noninfected controls. MicroRNAregulates gene expression post‐transcriptionally to influence biological processes. Cerebral malaria pathology caused mainly by the immunological disorder. We hypothesize that single‐nucleotide polymorphism in a microRNAinfluences microRNAbiogenesis or target gene recognition and altering susceptibility to cerebral malaria.

Methods

We performed a literature search based on immunological mechanism and applied microRNA‐related single‐nucleotide polymorphisms database to examine candidate microRNA SNPs possibly responsible for cerebral malaria. MicroRNA‐27a and microRNA‐146a are supposed to involve in cerebral malaria pathology. To assess the relationship of microRNA SNPto cerebral malaria outcome, we performed TaqMan Genotyping Assays in 110 cerebral malaria and 207 uncomplicated malaria cases for three candidate microRNA SNPs (rs895819 of microRNA‐27a, rs57095329 and rs2910164 of microRNA‐146a).

Results

Our study detected no significant difference in genotype and allele frequency of individual microRNA SNPs as well as in haplotypes of microRNA‐146a between these two groups of malaria patients in Thailand. Hardy–Weinberg disequilibrium of rs57095329 in the cerebral malaria group showed a heterozygous excess which might be due to natural selection.

Conclusion

Our data supported that the candidate microRNA SNPs have no major role to develop cerebral malaria.
Impact of gamma irradiation on virgin styrene butadiene rubber blended with ultrasonically devulcanized waste rubber
Authors: Elnaggar Mona Y. , Fathy El Sayed , Amdeha Enas , Hassan Medhat M.
Published: January 2019

This investigation focused on the opportunity of devulcanizing waste Rubber (WR) by ultrasonication to study the possibility of utilized as an added substance to replace with styrene‐butadiene rubber (SBR) in preparation process with the final aim of preparation new composites. The present work expects to compare the ultrasonic technique and the previous work on devulcanization by mechano‐chemical method. The influence of the ultrasonication treatment on the WR was explored by Fourier transform infrared, thermogravimetric analysis, scanning electron microscopy analysis revealed that ultrasonication was observed to be the best factor impacting the devulcanization procedure, this procedure suggests that use of specific levels of ultrasonic waves to the vulcanized rubber with a specific aim to accomplish a particular breaking of compound connections: scission of C‐S and S‐S bonds without break carbon–carbon (C‐C) bonds. Various blends of devulcanized WR with SBR was treatments with gamma ray then investigated and compared with find out the rubber giving the highest compatibility for compounding and revulcanization. In addition, a comparison of these results was made with the ones of raw SBR rubber compound. POLYM. ENG. SCI., 59:807–813, 2019. © 2019 Society of Plastics Engineers
The Role of Nerve Growth Factor in Maintaining Proliferative Capacity, Colony-Forming Efficiency, and the Limbal Stem Cell Phenotype
Authors: Kolli Sai , Bojic Sanja , Ghareeb Ali E. , Kurzawa-Akanbi Marzena , Figueiredo Francisco C. , Lako Majlinda
Published: January 2019

Nerve growth factor (NGF) has demonstrated great benefit in the treatment of neurotrophic corneal ulcers. There is evidence for multiple modes of action in promoting corneal healing, but only indirect evidence exists for NGF's effects on limbal stem cells (LSCs). Understanding the role of NGF in LSC biology will improve our understanding of paracrine regulation of the limbal niche and the design of stem cell-based therapies for conditions such as LSC deficiency. In this article, we studied the regulation of NGF signaling components during LSC differentiation and the role of NGF in LSC proliferation and maintenance of the stem cell phenotype. LSC differentiation was induced by prolonged (40 day) culture which resulted in a significant increase in cell size, decrease in colony-forming efficiency and expression of putative LSC markers. A protein microarray measuring expression of 248 signaling proteins indicated the low affinity NGF receptor p75NTR to be the most downregulated protein upon differentiation. Further confirmation by Western blotting and real-time quantitative polymerase chain reaction indicated that NGF and p75NTR are expressed in early LSC cultures and downregulated upon differentiation. LSC cultures grown in the presence of anti-NGF antibody showed decreased colony-forming efficiency, DNA replication and expression of putative LSC markers ABCG2 and C/EBPδ. Supplementation of LSC culture medium with NGF extended the life span of LSC cultures in vitro and increased the expression of putative LSC markers ΔNp63α and ABCG2. Taken together, our data indicate that NGF signaling is a key promoter of LSC proliferation, colony-forming efficiency, and a maintainer of the LSC phenotype. Stem Cells  2019;37:139–149
Bioenergetic Changes Underline Plasticity of Murine Embryonic Stem Cells
Authors: Vlaski-Lafarge Marija , Loncaric Darija , Perez Laura , Labat Véronique , Debeissat Christelle , Brunet de la Grange Philippe , Rossignol Rodrigue , Ivanovic Zoran , Bœuf Hélène
Published: January 2019

Murine embryonic stem cells (mESCs) are endowed by a time-dependent window of plasticity during their early commitment steps. Indeed, while mESCs deprived of leukemia inhibitory factor (LIF) for 24 hours revert to their naive pluripotent state after subsequent LIF readdition, cells deprived of LIF for 48 hours are no longer efficient in reverting, upon LIF addition, and undergo irreversible differentiation. We investigated undisclosed bioenergetic profiles of early mESC-derived committed cells versus their undifferentiated states in order to reveal specific bioenergetic changes associated with mESC plasticity. Multiparametric bioenergetic analysis revealed that pluripotent (+LIF) and reversibly committed cells (−LIF24h) are energetically flexible, depending on both oxidative phosphorylation (OXPHOS) and glycolysis. They exhibit high mitochondrial respiration in the presence of the main energetic substrates and can also rely on glycolysis in the presence of OXPHOS inhibitor. Inhibition of the glycolysis or mitochondrial respiration does not change drastically the expression of pluripotency genes, which remain well expressed. In addition, cells treated with these inhibitors keep their capacity to differentiate efficiently upon embryoid bodies formation. Transition from metabolically active mESCs to irreversibly committed cells is associated with a clear change in mitochondrial network morphology, to an increase of adenosine triphosphate (ATP) produced from glycolysis and a decline of ATP turnover and of the mitochondrial activity without change in the mitochondrial mass. Our study pointed that plasticity window of mESCs is associated with the bivalent energetic metabolism and potency to shift to glycolysis or OXPHOS on demand. LIF removal provokes glycolytic metabolic orientation and consecutive loss of the LIF-dependent reversion of cells to the pluripotent state. Stem Cells  2019;37:463–475
Iron Homeostasis Determines Fate of Human Pluripotent Stem Cells Via Glycerophospholipids-Epigenetic Circuit
Authors: Han Zhenbo , Yu Ying , Xu Juan , Bao Zhengyi , Xu Zihang , Hu Jiancheng , Yu Meixi , Bamba Djibril , Ma Wenya , Ding Fengzhi , Zhang Lai , Jin Mengyu , Yan Gege , Huang Qi , Wang Xiuxiu , Hua Bingjie , Yang Fan , Li Yuan , Lei Lei , Cao Nan , Pan Zhenwei , Cai Benzhi
Published: January 2019

Iron homeostasis is crucial for a variety of biological processes, but the biological role of iron homeostasis in pluripotent stem cells (PSCs) remains largely unknown. The present study aimed to determine whether iron homeostasis is involved in maintaining the pluripotency of human PSCs (hPSCs). We found that the intracellular depletion of iron leads to a rapid downregulation of NANOG and a dramatic decrease in the self-renewal of hPSCs as well as spontaneous and nonspecific differentiation. Moreover, long-term depletion of iron can result in the remarkable cell death of hPSCs via apoptosis and necrosis pathways. Additionally, we found that the depletion of iron increased the activity of lipoprotein-associated phospholipase A2 (LP-PLA2) and the production of lysophosphatidylcholine, thereby suppressing NANOG expression by enhancer of zeste homolog 2-mediated trimethylation of histone H3 lysine 27. Consistently, LP-PLA2 inhibition abrogated iron depletion-induced loss of pluripotency and differentiation. Altogether, the findings of our study demonstrates that iron homeostasis, acting through glycerophospholipid metabolic pathway, is essential for the pluripotency and survival of hPSCs. Stem Cells  2019;37:489–503
Chemoprevention with Somatuline© Delays the Progression of Pancreatic Neuroendocrine Neoplasms in a Mouse Model of Multiple Endocrine Neoplasia Type 1 (MEN1)
Authors: Lopez Caroline L. , Joos Barbara , Bartsch Detlef K. , Manoharan Jerena , Albers Max , Slater Emily P. , Bollmann Carmen , Roth Sylvia , Bayer Aninja , Fendrich Volker
Published: January 2019

Objective

Long‐acting synthetic somatostatin analogues (SSA) are an essential part of the treatment of neuroendocrine neoplasms. We evaluated the chemopreventive effects of a long‐acting somatostatin analogue on the development of pancreatic neuroendocrine neoplasms (pNENs) in a genetically engineered MEN1 knockout mouse model.

Materials and methods

Heterozygote MEN1 knockout mice were injected every 28 days subcutaneously with the somatostatin analogue lanreotide (Somatuline Autogel©; Ipsen Pharma) or a placebo starting at day 35 after birth. Mice were euthanized after 6, 9, 12, 15 and 18 months, and the size and number of pNENs were measured due histological analysis and compared to the placebo group.

Results

The median tumor size of pNENs was statistically significantly smaller after 9 (control group vs. SSA group; 706.476 µm2 vs. 195.271 µm2; p = 0.0012), 12 (placebo group vs. SSA group 822.022 vs. 255.482; p ≤ 0.001), 15 (placebo group vs. SSA group 1192.568 vs. 273.533; p ≤ 0.001) and after 18 months (placebo group vs. SSA group 1328.299 vs. 864.587; p ≤ 0.001) in the SSA group. Comparing the amount of tumors in both groups, a significant reduction was achieved in treated Men1(+/−) mice (41%, p = 0.002). Immunostaining showed, however, no significant difference in the expression of the apoptosis marker caspase‐3, but a significant difference in Ki67 index as a marker for tumor cell proliferation (p ≤ 0.005).

Conclusion

Long‐acting somatostatin analogues may be an effective chemopreventive approach to delay the progression of MEN1‐associated pNENs. After our preclinical results, we would recommend to evaluate the effects of long‐acting SSA in a prospective clinical trial.