bluuebunny/crossref_metadata_2025 huggingface dataset:
Mono Camera-Based Optical Vehicular Communication for an Advanced Driver Assistance System
Authors: Ali Md. Osman , Ahmed Md. Faisal , Hasan Moh. Khalid , Shahjalal Md. , Rahman Md. Habibur , Jahan Israt , Jang Yeong Min
Published: June 2021

This technical paper proposes a new waveform combining the low-rate and high-rate data streams to detect the region-of-interest signal in a high-mobility environment using optical camera communication. The proposed technique augments the bit rate of the low-rate stream; consequently, the link setup time is reduced and the requirement of low frame rate camera is eliminated. Additionally, both the low-rate and high-rate data streams in the proposed bi-level pulse position modulation are decoded with a unique adaptive thresholding mechanism with a high frame rate camera. We also propose a vehicle localization scheme to assist the drivers in maintaining a safe following distance that can significantly reduce the frequency of accidents. Moreover, two neural networks are proposed to detect the light-emitting diodes (LEDs) for localization and communication, and to estimate the road curvature from different rear LED shapes of the forwarding vehicle, respectively. The system is implemented, and its performance is analyzed in Python 3.7. The implementation results show that the proposed system is able to achieve 75% localization accuracy, a 150 bps low-rate stream, and a 600 bps high-rate stream over a range of 25 m with a commercial 30 fps camera.
Evaluation of Flicker of Light Generated by Arc Furnaces
Authors: Olczykowski Zbigniew , Łukasik Zbigniew
Published: June 2021

Due to the dynamic nature of load changes, arc devices are receivers that generate disturbances to the network that affect the power quality. The main disturbance generated by these receivers are voltage fluctuations. One of the effects of voltage fluctuations is the flicker of light caused by lighting receivers. The article presents an analysis of changes indicators flicker of light measured in networks supplying arc furnaces. The propagation of voltage fluctuations to the lines supplying lighting receivers was analyzed. The network parameters influencing the amount of light flicker were estimated. The paper presents a method for calculating the increased flicker of light when several electric arc furnaces are operated in parallel. The conclusions regarding the use of the presented research in practical applications are given in the summary.
SWAT Model Adaptability to a Small Mountainous Forested Watershed in Central Romania
Authors: Tudose Nicu Constantin , Marin Mirabela , Cheval Sorin , Ungurean Cezar , Davidescu Serban Octavian , Tudose Oana Nicoleta , Mihalache Alin Lucian , Davidescu Adriana Agafia
Published: June 2021

This study aims to build and test the adaptability and reliability of the Soil and Water Assessment Tool hydrological model in a small mountain forested watershed. This ungauged watershed covers 184 km2 and supplies 90% of blue water for the Brașov metropolitan area, the second largest metropolitan area of Romania. After building a custom database at the forest management compartment level, the SWAT model was run. Further, using the SWAT-CUP software under the SUFI2 algorithm, we identified the most sensitive parameters required in the calibration and validation stage. Moreover, the sensitivity analysis revealed that the surface runoff is mainly influenced by soil, groundwater and vegetation condition parameters. The calibration was carried out for 2001–2010, while the 1996–1999 period was used for model validation. Both procedures have indicated satisfactory performance and a lower uncertainty of model results in replicating river discharge compared with observed discharge. This research demonstrates that the SWAT model can be applied in small ungauged watersheds after an appropriate parameterisation of its databases. Furthermore, this tool is appropriate to support decision-makers in conceiving sustainable watershed management. It also guides prioritising the most suitable measures to increase the river basin resilience and ensure the water demand under climate change.
Abundant Wave Accurate Analytical Solutions of the Fractional Nonlinear Hirota–Satsuma–Shallow Water Wave Equation
Authors: Yue Chen , Lu Dianchen , Khater Mostafa M. A.
Published: June 2021

This research paper targets the fractional Hirota’s analytical solutions–Satsuma (HS) equations. The conformable fractional derivative is employed to convert the fractional system into a system with an integer–order. The extended simplest equation (ESE) and modified Kudryashov (MKud) methods are used to construct novel solutions of the considered model. The solutions’ accuracy is investigated by handling the computational solutions with the Adomian decomposition method. The solutions are explained in some different sketches to demonstrate more novel properties of the considered model.
Adaptive Wavelet Methods for Earth Systems Modelling
Authors: Kevlahan Nicholas K.-R.
Published: June 2021

This paper reviews how dynamically adaptive wavelet methods can be designed to simulate atmosphere and ocean dynamics in both flat and spherical geometries. We highlight the special features that these models must have in order to be valid for climate modelling applications. These include exact mass conservation and various mimetic properties that ensure the solutions remain physically realistic, even in the under-resolved conditions typical of climate models. Particular attention is paid to the implementation of complex topography in adaptive models. Using wavetrisk as an example, we explain in detail how to build a semi-realistic global atmosphere or ocean model of interest to the geophysical community. We end with a discussion of the challenges that remain to developing a realistic dynamically adaptive atmosphere or ocean climate models. These include scale-aware subgrid scale parameterizations of physical processes, such as clouds. Although we focus on adaptive wavelet methods, many of the topics we discuss are relevant for adaptive mesh refinement (AMR).
Combination of Direct Viable Count and Fluorescent In Situ Hybridization (DVC-FISH) as a Potential Method for Identifying Viable Vibrio parahaemolyticus in Oysters and Mussels
Authors: García-Hernández Jorge , Hernández Manuel , Moreno Yolanda
Published: June 2021

Vibrio parahaemolyticus is a human food-borne pathogen with the ability to enter the food chain. It is able to acquire a viable, non-cultivable state (VBNC), which is not detected by traditional methods. The combination of the direct viable count method and a fluorescent in situ hybridization technique (DVC-FISH) makes it possible to detect microorganisms that can present VBNC forms in complex samples The optimization of the in vitro DVC-FISH technique for V. parahaemolyticus was carried out. The selected antibiotic was ciprofloxacin at a concentration of 0.75 μg/mL with an incubation time in DVC broth of 5 h. The DVC-FISH technique and the traditional plate culture were applied to detect and quantify the viable cells of the affected pathogen in artificially contaminated food matrices at different temperatures. The results obtained showed that low temperatures produced an important logarithmic decrease of V. parahaemolyticus, while at 22 °C, it proliferated rapidly. The DVC-FISH technique proved to be a useful tool for the detection and quantification of V. parahaemolyticus in the two seafood matrices of oysters and mussels. This is the first study in which this technique has been developed to detect viable cells for this microorganism.
Potential In Vitro Inhibition of Selected Plant Extracts against SARS-CoV-2 Chymotripsin-Like Protease (3CLPro) Activity
Authors: Guijarro-Real Carla , Plazas Mariola , Rodríguez-Burruezo Adrián , Prohens Jaime , Fita Ana
Published: June 2021

Antiviral treatments inhibiting Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication may represent a strategy complementary to vaccination to fight the ongoing Coronavirus disease 19 (COVID-19) pandemic. Molecules or extracts inhibiting the SARS-CoV-2 chymotripsin-like protease (3CLPro) could contribute to reducing or suppressing SARS-CoV-2 replication. Using a targeted approach, we identified 17 plant products that are included in current and traditional cuisines as promising inhibitors of SARS-CoV-2 3CLPro activity. Methanolic extracts were evaluated in vitro for inhibition of SARS-CoV-2 3CLPro activity using a quenched fluorescence resonance energy transfer (FRET) assay. Extracts from turmeric (Curcuma longa) rhizomes, mustard (Brassica nigra) seeds, and wall rocket (Diplotaxis erucoides subsp. erucoides) at 500 µg mL−1 displayed significant inhibition of the 3CLPro activity, resulting in residual protease activities of 0.0%, 9.4%, and 14.9%, respectively. Using different extract concentrations, an IC50 value of 15.74 µg mL−1 was calculated for turmeric extract. Commercial curcumin inhibited the 3CLPro activity, but did not fully account for the inhibitory effect of turmeric rhizomes extracts, suggesting that other components of the turmeric extract must also play a main role in inhibiting the 3CLPro activity. Sinigrin, a major glucosinolate present in mustard seeds and wall rocket, did not have relevant 3CLPro inhibitory activity; however, its hydrolysis product allyl isothiocyanate had an IC50 value of 41.43 µg mL−1. The current study identifies plant extracts and molecules that can be of interest in the search for treatments against COVID-19, acting as a basis for future chemical, in vivo, and clinical trials.
Enhanced Mechanical Properties by Ionomeric Complexation in Interpenetrating Network Hydrogels of Hydrolyzed Poly (N-vinyl Formamide) and Polyacrylamide
Authors: Scalet Joseph M. , Suekama Tiffany C. , Jeong Jeayoung , Gehrke Stevin H.
Published: June 2021

Tough hydrogels were made by hydrolysis of a neutral interpenetrating network (IPN) of poly (N-vinyl formamide) PNVF and polyacrylamide (PAAm) networks to form an IPN of polyvinylamine (PVAm) and poly (acrylic acid) (PAAc) capable of intermolecular ionic complexation. Single network (SN) PAAm and SN PNVF have similar chemical structures, parameters and physical properties. The hypothesis was that starting with neutral IPN networks of isomeric monomers that hydrolyze to comparable extents under similar conditions would lead to formation of networks with minimal phase separation and maximize potential for charge–charge interactions of the networks. Sequential IPNs of both PNVF/PAAm and PAAm/PNVF were synthesized and were optically transparent, an indication of homogeneity at submicron length scales. Both IPNs were hydrolyzed in base to form PVAm/PAAc and PAAc/PVAm IPNs. These underwent ~5-fold or greater decrease in swelling at intermediate pH values (3–6), consistent with the hypothesis of intermolecular charge complexation, and as hypothesized, the globally neutral, charge-complexed gel states showed substantial increases in failure properties upon compression, including an order of magnitude increases in toughness when compared to their unhydrolyzed states or the swollen states at high or low pH values. There was no loss of mechanical performance upon repeated compression over 95% strain.
Visual Algorithm of VR E-Sports for Online Health Care
Authors: Lim Sang-Guk , Jung Se-Hoon , Huh Jun-Ho
Published: June 2021

The need for non-face-to-face online health care has emerged through the era of “untact”. However, there is a lack of standardization work and research cases on the exercise effect of immersive content. In this study, the possibility of the exercise effect of VR e-sports among e-sports cases were presented through a visual algorithm analysis. In addition, the evaluation criteria were established. The research method compares and analyzes e-sports cases and VR e-sports cases by applying existing evaluation research cases. It also sets up a new evaluation standard. As for the analysis result, the device immersion method and interaction range were set through an algorithm analysis; FOV and frame immersion were set through typification; the user recognition method and interaction method were set through the visual diagram. Then, each derived result value was quantified and a new evaluation criterion was proposed.
Traumatic Events and Eagle Syndrome: Is There Any Correlation? A Systematic Review
Authors: Saccomanno Sabina , Quinzi Vincenzo , D’Andrea Nicola , Albani Arianna , Coceani Paskay Licia , Marzo Giuseppe
Published: June 2021

Background: Eagle syndrome occurs when elongated styloid process or ossification of the stylohyoid ligament interfere with the surrounding anatomical structures giving rise to various symptoms. Watt W. Eagle identified two types: stylo-hyoid classic syndrome and stylo-carotid artery syndrome. The aim of this systematic review of the literature is to evaluate correlations between Eagle syndrome and traumatic events or teeth extractions. Methods: out of 294 articles, the final study allowed the identification of 13 studies focusing on traumatic events. Out of 342 articles, the final study allowed the analysis of two studies regarding extractive dental events. Results: 13 articles showed correlations between the onset of symptoms in Eagle syndrome and traumatic events and highlighted two possibilities: traumatic event could fracture the already elongated styloid process or calcified stylohyoid ligament; trauma itself triggers the pathophysiological mechanisms that lead to lengthening of styloid process or calcification of stylohyoid ligament and therefore the typical symptoms. The only two case reports concerning Eagle syndrome symptoms after extractive dental events describe the onset of classic type. Conclusions: The analyzed articles confirm correlation between traumatic event and onset of typical symptoms of Eagle syndrome. There is not enough literature linking extractive dental events to Eagle syndrome. Trial registration: CRD42020185176.
Sensory Profile, Shelf Life, and Dynamics of Bioactive Compounds during Cold Storage of 17 Edible Flowers
Authors: Demasi Sonia , Mellano Maria Gabriella , Falla Nicole Mélanie , Caser Matteo , Scariot Valentina
Published: June 2021

In this study, 17 edible flowers (Allium ursinum L., Borago officinalis L., Calendula officinalis L., Centaurea cyanus L., Cichorium intybus L., Dianthus carthusianorum L., Lavandula angustifolia Mill., Leucanthemum vulgare (Vaill.) Lam., Paeonia officinalis L., Primula veris L., Robinia pseudoacacia L., Rosa canina L., Rosa pendulina L., Salvia pratensis L., Sambucus nigra L., Taraxacum officinale Weber, and Tropaeolum majus L.) were investigated to assess their sensory profile at harvest and their shelf life and bioactive compounds dynamics during cold storage. The emerging market of edible flowers lacks this information; thus, the characteristics and requirements of different flower species were provided. In detail, a quantitative descriptive analysis was performed by trained panelists at flower harvest, evaluating 10 sensory descriptors (intensity of sweet, sour, bitter, salt, smell, specific flower aroma, and herbaceous aroma; spiciness, chewiness, and astringency). Flower visual quality, biologically active compounds content (total polyphenols and anthocyanins), and antioxidant activity (FRAP, DPPH, and ABTS assays) were evaluated both at harvest and during storage at 4 °C for 14 days to assess their shelf life. Generally, species had a wide range of peculiar sensory and phytochemical characteristics at harvest, as well as shelf life and bioactive compounds dynamics during postharvest. A strong aroma was indicated for A. ursinum, D. carthusianorum, L. angustifolia, and L. vulgare, while B. officinalis and C. officinalis had very low values for all aroma and taste descriptors, resulting in poor sensory profiles. At harvest, P. officinalis, R. canina, and R. pendulina exhibited the highest values of polyphenols (884–1271 mg of gallic acid equivalents per 100 g) and antioxidant activity (204–274 mmol Fe2+/kg for FRAP, 132–232 and 43–58 µmol of Trolox equivalent per g for DPPH and ABTS). The species with the longest shelf life in terms of acceptable visual quality was R. pendulina (14 days), followed by R. canina (10 days). All the other species lasted seven days, except for C. intybus and T. officinale that did not reach day 3. During cold storage, the content of bioactive compounds differed, as total phenolics followed a different trend according to the species and anthocyanins remained almost unaltered for 14 days. Considering antioxidant activity, ABTS values were the least variable, varying in only four species (A. ursinum, D. carthusianorum, L. angustifolia, and P. officinalis), while both DPPH and FRAP values varied in eight species. Taken together, the knowledge of sensory profiles, phytochemical characteristics and shelf life can provide information to select suitable species for the emerging edible flower market.
A Wireless Fingerprint Positioning Method Based on Wavelet Transform and Deep Learning
Authors: Li Da , Niu Zhao
Published: June 2021

As the demand for location services increases, research on location technology has aroused great interest. In particular, signal-based fingerprint location positioning technology has become a research hotspot owing to its high positioning performance. In general, the received signal strength indicator (RSSI) will be used as a location feature to build a fingerprint database. However, at different locations, this feature distinction may not be obvious, resulting in low positioning accuracy. Considering the wavelet transform can get valuable features from the signals, the long-term evolution (LTE) signals were converted into wavelet feature images to construct the fingerprint database. To fully extract the signal features, a two-level hierarchical structure positioning system is proposed to achieve satisfactory positioning accuracy. A deep residual network (ResNet) rough locator is used to learn useful features from the wavelet feature fingerprint image database. Then, inspired by the transfer learning idea, a fine locator based on multilayer perceptron (MLP) is leveraged to further learn the features of the wavelet fingerprint image to obtain better localization performance. Additionally, multiple data enhancement techniques were adopted to increase the richness of the fingerprint dataset, thereby enhancing the robustness of the positioning system. Experimental results indicate that the proposed system leads to improved positioning performance in outdoor environments.
Selection and Validation of Reference Genes for Gene Expression Analysis in Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)
Authors: Yan Xin , Zhang Yibo , Xu Kangkang , Wang Yawei , Yang Wenjia
Published: June 2021

The tomato leaf miner, Tuta absoluta is a destructive pest of tomato. The leaf-mining activities of its larvae can cause significant yield losses. Real-time quantitative polymerase chain reaction (RT-qPCR) is commonly used to measure gene expression, and the selection of stable reference genes for calibration and standardization is critical for accurate use of RT-qPCR. We studied the stable expression of nine common housekeeping genes in T. absoluta. These were examined at different developmental stages, in larval tissues, as well as those induced by exposure to 20E and insecticides. Four dedicated algorithms (geNorm, BestKeeper, NormFinder, and ΔCt method) and online tool (RefFinder) were used to analyze and rank the tested reference genes. Based on the standardized gene expression data of target gene ecdysone receptor (EcR), the applicability of specific reference genes was verified. The results clarify that the optimal internal reference genes vary greatly under different experimental conditions. GAPDH and RPS11 were the best reference genes for developmental stages; RPL28 and RPL10 for different tissues; EF1α and RPL28 for 20E treatment; EF1α and RPL7A for insecticide treatments. The most suitable reference genes in all experimental conditions are EF1α and RPL28.
Impact of Weight on Clinical Outcomes of Edoxaban Therapy in Atrial Fibrillation Patients Included in the ETNA-AF-Europe Registry
Authors: Boriani Giuseppe , De Caterina Raffaele , Manu Marius Constantin , Souza José , Pecen Ladislav , Kirchhof Paulus
Published: June 2021

Background: Extremes of body weight may alter exposure to non-vitamin K antagonist oral anticoagulants and thereby impact clinical outcomes. This ETNA-AF-Europe sub-analysis assessed 1-year outcomes in routine care patients with atrial fibrillation across a range of body weight groups treated with edoxaban. Methods: ETNA-AF-Europe is a multinational, multicentre, observational study conducted in 825 sites in 10 European countries. Overall, 1310, 5565, 4346 and 1446 enrolled patients were categorised into ≤60 kg, >60–≤80 kg (reference weight group), >80–≤100 kg and >100 kg groups. Results: Patients weighing ≤60 kg were older, more frail and had a higher CHA2DS2-VASc score vs. the other weight groups. The rates of stroke/systemic embolism, major bleeding and ICH were low at 1 year (0.82, 1.05 and 0.24%/year), with no significant differences among weight groups. The annualised event rates of all-cause death were 3.50%/year in the overall population. After adjustment for eGFR and CHA2DS2-VASc score, the risk of all-cause death was significantly higher in extreme weight groups vs. the reference group. Conclusions: Low rates of stroke and bleeding were reported with edoxaban, independent of weight. The risk of all-cause death was higher in extremes of weight vs. the reference group after adjustment for important risk modifiers, thus no obesity paradox was observed.
Higher Periwound Temperature Associated with Wound Healing of Pressure Ulcers Detected by Infrared Thermography
Authors: Lin Yen-Hsi , Chen Yen-Chin , Cheng Kuo-Sheng , Yu Po-Jui , Wang Jiun-Ling , Ko Nai-Ying
Published: June 2021

Visual and empirical assessments do not enable the early detection of wound deterioration or necroses. No suitable objective indicator for predicting poor wound-healing is currently available. We used infrared thermography to determine the association between wound temperature and pressure-wound healing. We examined patients with grades 2–4 pressure ulcers from a medical center in southern Taiwan and recorded the temperatures of the wound bed, periwound, and normal skin using infrared thermographic cameras. A total of 50 pressure ulcers and 248 infrared-thermography temperature records were analyzed. Normal skin temperature was not related to pressure ulcer wound healing. In a multivariate analysis, higher malnutrition universal-screening-tool scores were associated with poor wound-healing (p = 0.020), and higher periwound-temperature values were associated with better wound-healing (p = 0.028). In patients who had higher periwound-skin temperature than that of the wound bed, that result was also associated with better wound-healing (p = 0.002). Wound-bed and periwound temperatures differed significantly with the grade of the pressure ulcer, and a high periwound temperature was positively correlated with wound healing. Infrared thermography can objectively serve as indicators for assessing pressure-ulcer healing.
Prevalence of Chronic Kidney Disease in Patients with Diabetes in Extremadura (Spain) during the Years 2012, 2013 and 2014: An Observational Study
Authors: Fernández-Fernández Leandro , Barquilla-García Alfonso , Sánchez-Vega Javier , Risco-Solanilla José Carlos , Suárez-González Félix , Buitrago Francisco
Published: June 2021

Diabetes mellitus (DM) is one of the leading causes of chronic kidney disease (CKD). We analyzed the prevalence of CKD in the population with diabetes in Extremadura (Spain). retrospective observational study was carried in the diabetic population attended in the Extremadura Health System in 2012–2014. A total of 38,253 patients, ≥18 years old were included. Estimated glomerular filtration rate (eGFR) was calculated using the CKD Epidemiology Collaboration equation. CKD was defined as follow: an eGFR <60 mL/min/1.73 m2 in a time period ≥ of three months or the presence of renal damage, with or without reduced eGFR, if the urine albumin-creatinine ratio (UACR) was ≥30 mg/g, also in a time period ≥ of three months. The prevalence rate of CKD was 25.3% (27.6% in women; 23.0% in men) and increases with age (34.0% in ≥65 years-olds). 24.9% of patients with CKD were in the very-high risk category for cardiovascular events (6.3% of the diabetic population). If CKD were diagnosed without requiring sustained eGFR <60 mL/min/1.73 m2 and/or sustained UACR ≥30 mg/g (as it is frequently found in the literature) this would overestimate the prevalence of CKD by 23%.
Complement Inhibition for Geographic Atrophy: A Tempting Target with Mixed Results
Authors: Lin Jonathan B. , Halawa Omar A. , Miller Joan W. , Vavvas Demetrios G.
Published: June 2021

Age-related macular degeneration (AMD) is a leading cause of blindness in older adults. One of the strongest genetic risk factors for AMD is a complement factor H (CFH) gene polymorphism characterized by a tyrosine-histidine change at amino acid position 402 (Y402H). The magnitude of this association between the Y402H variant and AMD is among the strongest that has been identified for any complex, multifactorial human disease. This strong association has motivated researchers to investigate a potential link between various elements of the complement pathway and AMD pathogenesis. Given the possible contribution of complement dysregulation to AMD, complement inhibition has emerged as a therapeutic strategy for slowing geographic atrophy (GA). Randomized clinical trials thus far have yielded mixed results. In this article, we provide the historical context for complement inhibition as a strategy for treating GA, discuss potential advantages and disadvantages of complement inhibition, and highlight the questions that must be addressed before complement inhibition can take center stage as a therapy for AMD.
Efficacy of Over-The-Scope Clip Method as a Novel Hemostatic Therapy for Colonic Diverticular Bleeding
Authors: Kawano Koichiro , Takenaka Mamoru , Kawano Reiko , Kagoshige Daisuke , Kawase Yuta , Moriguchi Tomonori , Tanabe Hiroshi , Katoh Takao , Nishi Katsuhisa , Kudo Masatoshi
Published: June 2021

Colonic diverticular could bleed recurrently, and, sometimes, fatal massive bleeding could occur. However, the choice of endoscopic hemostasis remains controversial. Although the over-the-scope clip (OTSC) method has been reported to be effective, it has not been fully evaluated due to the small number of cases. This study aimed to evaluate the efficacy of the OTSC method for colonic diverticular bleeding. Between August 2017 and December 2020, 36 consecutive patients, including those who could not be treated using endoscopic band ligation (EBL) and those in whom re-bleeding had occurred after EBL, underwent the OTSC method for hemostasis of colonic diverticular bleeding at Hyogo Prefectural Awaji Medical Center. The procedure success rate, adverse events rate, early phase re-bleeding rate (within 30 days following primary hemostasis), and the requirement rate for additional transcatheter arterial embolization (TAE) or surgery were the outcomes assessed. The outcomes were procedure success rate 100%, adverse events rate 0%, early phase re-bleeding rate 8.3%, and additional TAE or surgery rate 0%. These results suggest that the OTSC method is a safe and effective treatment for managing colonic diverticular bleeding.
Spectral-Domain Optical Coherence Tomography Assessment in Treatment-Naïve Patients with Clinically Isolated Syndrome and Different Multiple Sclerosis Types: Findings and Relationship with the Disability Status
Authors: Rzepiński Łukasz , Kucharczuk Jan , Maciejek Zdzisław , Grzybowski Andrzej , Parisi Vincenzo
Published: June 2021

This study evaluates the peripapillary retinal nerve fiber layer (pRNFL) thickness and total macular volume (TMV) using spectral-domain optical coherence tomography in treatment naïve patients with the clinically isolated syndrome (CIS) and different multiple sclerosis (MS) types. A total of 126 patients (15 CIS, 65 relapsing-remitting MS, 14 secondary progressive MS, 11 primary progressive MS, 21 benign MS) with or without optic neuritis (ON) history and 63 healthy age-similar controls were assessed. Concerning controls’ eyes, pRNFL thickness was significantly reduced in CIS-ON eyes (p < 0.01), while both TMV and pRNFL thickness was decreased in all MS eyes regardless of ON history (p < 0.01). Significant differences in pRNFL thickness and TMV between MS variants were observed for non-ON eyes (p < 0.01), with the lowest values in benign and secondary progressive disease type, respectively. The pRNFL thickness was inversely correlated with Expanded Disability Status Scale (EDSS) score in non-ON subgroups (p < 0.01), whereas TMV was inversely correlated with EDSS score in both ON and non-ON subgroups (p < 0.01). Concluding, pRNFL thinning confirms optic nerve damage in CIS-ON eyes and appears to be disproportionately high with respect to the disability status of benign MS patients. The values of TMV and pRNFL in non-ON eyes significantly correspond to MS course heterogeneity and patients’ disability than in ON eyes.
Hearing Aid Fitting in Tinnitus: A Scoping Review of Methodological Aspects and Effect on Tinnitus Distress and Perception
Authors: Kikidis Dimitrios , Vassou Evgenia , Markatos Nikolaos , Schlee Winfried , Iliadou Eleftheria
Published: June 2021

Current evidence on efficacy of hearing aids (HAs) on tinnitus perception and annoyance is considered insufficient due to the heterogeneity of tinnitus characteristics and of methods used in the relevant clinical studies. This is a scoping review focused on the methodological aspects of clinical studies evaluating the value of HA fitting as part of tinnitus management over the past 10 years. Thirty-four studies were included in the review, showing important heterogeneity in almost all aspects of inclusion criteria, comparators, outcome measures, follow-up time and HA fitting procedures. Although all studies show that HA fitting has a positive impact on tinnitus perception in patients with hearing loss, the methodological heterogeneity does not allow robust conclusions. Future studies taking into account the different nature and goals of each tinnitus therapeutic modality and adapting their methods, endpoints and timelines according to them could lay the groundwork for obtaining high-quality evidence on whether and how HA fitting shall be implemented in tinnitus management strategies.
ISIDOG Consensus Guidelines on COVID-19 Vaccination for Women before, during and after Pregnancy
Authors: Donders Gilbert , Grinceviciene Svitrigaile , Haldre Kai , Lonnee-Hoffmann Risa , Donders Francesca , Tsiakalos Aristotelis , Adriaanse Albert , Martinez de Oliveira José , Ault Kevin , Mendling Werner ,   on the behalf of the COVID-19 ISIDOG Guideline Group
Published: June 2021

Introduction. Sars-CoV-2 infection poses particular problems in pregnancy, as the infection more frequently causes severe complications than in unaffected pregnant women or nonpregnant women with SARS-CoV-2 infection. Now that vaccination is available and rapidly being implemented worldwide, the question arises whether pregnant women should be vaccinated, and if so, whether they should receive priority. Methods. Available scientific data and available guidelines about vaccination against SARS-CoV-2 were collected by the Guideline Committee of the International Society of Infectious Diseases in Obstetrics and Gynecology (ISIDOG) and were analyzed, discussed and summarized as guidelines for healthcare workers caring for pregnant women. Concluding statements were graded according to the Oxford evidence-based medicine grading system. Results. There is evidence to consider pregnancy as a risk factor for serious complications of COVID-19 infection, even in the absence of additional risk factors, such as hypertension, diabetes and obesity which increase these risks even more in pregnancy. Currently available data slightly favor mRNA-based vaccines above vector-based vaccines during pregnancy and breastfeeding, until more safety data become available. Conclusion. ISIDOG advises policy makers and societies to prioritize pregnant women to receive vaccination against SARS-CoV-2 and favor the mRNA vaccines until further safety information becomes available.
The Relationship between Adjacent Segment Pathology and Facet Joint Violation by Pedicle Screw after Posterior Lumbar Instrumentation Surgery
Authors: Oh Ho-Seok , Seo Hyoung-Yeon
Published: June 2021

Transpedicular screw instrumentation systems have been increasingly utilized during the fusion of lumbar spine procedures. The superior segment facet joint violation of the pedicle screw is thought to have potential for accelerating symptomatic adjacent-segment pathology (ASP). The purpose of this study was to investigate the relationship between the superior segment facet joint violation by transpedicular screws and the development of ASP. Among all patients who underwent operations involving one- or two-level posterior lumbar arthrodesis at the Chonnam National University Hospital from 1992 to 2012, 87 patients were selected for this study. Fifty-six patients were included in the ASP group, and 31 were included in the non-ASP group. We used lumbar three-dimensional computed tomography (CT) to assess the violation of the superior facet joint by a transpedicular screw. The assessment is presented in scores ranging from zero to two, with zero indicating no violation (type I); one point indicating suspected violation (type II); and two points indicating definitely facet joint violation (type III). Facet violation was reported in 31 patients in the ASP group (n = 56), and in 13 patients in the non-ASP group (n = 31). The types of facet joint violation according to our scoring system were as follows: type I, 59 screws (52.7%); type II, 26 screws (23.2%); and type III, 27 screws (24.1%) in the ASP group; and type I, 43 screws (69.4%), type II, 14 screws (22.6 %); and type III, 5 screws (8.0%) in the non-ASP group. The score of facet joint violation in each patient according to our scoring system were as follows: 0 points, 25 patients (44.6%); 1 point, 8 patients (14.3%); 2 points, 4 patients (7.1%); 3 points, 11 patients (19.7%); 4 points, 8 patients (14.3%) in the ASP group; and 0 points, 18 patients (58.1%); 1 point, 4 patients (12.9%); 2 points, 7 patients (22.6%); 3 points, 2 patients (6.4%); 4 points, 0 patients (0%) in the non-ASP group. The mean scores were 1.4 points in the ASP group and 0.8 points in the non-ASP group (p < 0.05). We conclude that the position of the pedicle screw farther away from the facet joint surface can reduce the degeneration of the superior adjacent segment. Therefore, close attention to the screw position during surgery may reduce the rate of superior adjacent-segment pathologies.
The Outcomes of Cochlear Implantation in Usher Syndrome: A Systematic Review
Authors: Davies Camron , Bergman Jenna , Misztal Carly , Ramchandran Renuka , Mittal Jeenu , Bulut Erdogan , Shah Viraj , Mittal Rahul , Eshraghi Adrien A.
Published: June 2021

Objective: To systematically appraise the implementation of cochlear implantation (CI) in Usher Syndrome (USH) Types 1, 2, and 3 patients, and analyze who would benefit from CI. Data Sources: A comprehensive search of PubMed, Embase, CINAHL, and Cochrane Library electronic databases from inception through June 2020 was performed. There were no language restrictions. Study Selection: The PRISMA strategy was followed. Included studies discuss USH patients who underwent CI regardless of age, nationality, or clinical subtype. All included studies report post-implantation functional, cognitive, or quality of life outcomes. Only reviews were excluded. Results: Fifteen studies met the inclusion criteria. USH patients experienced improvements in PTA and speech perception and expression outcomes after CI, as well as improvements in phonological memory and quality of life measures. Overall, patients implanted at younger ages outperformed older patients in audiological testing. Similarly, patients with prolonged auditory deprivation had relatively poor performance outcomes in sentence recognition and speech detection following CI. Conclusions: Most USH patients benefit from CI. USH patients who undergo CI at younger ages generally achieve better hearing, speech, and cognitive outcomes. CI at older ages can still prove beneficial if appropriate auditory amplification is started at the right time. Further research is warranted to fill the gap in understanding regarding the gene mutations underlying the pathophysiology of USH that have favorable CI outcomes as well as the optimal time to perform CI.
Extracellular Water to Total Body Water Ratio in Septic Shock Patients Receiving Protocol-Driven Resuscitation Bundle Therapy
Authors: Chae Bora , Shin Yo Sep , Hong Seok-In , Kim Sang Min , Kim Youn-Jung , Ryoo Seung Mok , Kim Won Young
Published: June 2021

(1) Bio-electrical impedance analysis (BIA) is a rapid, simple, and noninvasive tool for evaluating the metabolic status and for assessing volume status in critically ill patients. Little is known, however, the prognostic value of body composition analysis in septic shock patients. This study assessed the association between parameters by body composition analysis and mortality in patients with septic shock in the emergency department (ED). (2) Data were prospectively collected on adult patients with septic shock who underwent protocol-driven resuscitation bundle therapy between December 2019 and January 2021. The primary outcome was 30-day mortality. (3) The study included 261 patients, the average ratio of extracellular water (ECW) to total body water (TBW) was significantly higher in non-survivors than in survivors (0.414 vs. 0.401, p < 0.001). Multivariate analysis showed that ECW/TBW ≥ 0.41 (odds ratio (OR), 4.62; 95% confidence interval (CI), 2.31–9.26, p < 0.001), altered mental status (OR, 2.88; 95% CI, 1.28–6.46, p = 0.010), and lactate level (OR, 1.24; 95% CI, 1.12–1.37, p < 0.001) were significantly associated with 30-day mortality in patients with septic shock. (4) ECW/TBW ≥ 0.41 may be associated with 30-day mortality in patients with septic shock receiving protocol-driven resuscitation bundle therapy in the ED.
Exhaled-Breath Testing Using an Electronic Nose during Spinal Cord Stimulation in Patients with Failed Back Surgery Syndrome: An Experimental Pilot Study
Authors: Goudman Lisa , Jansen Julie , Vets Nieke , De Smedt Ann , Moens Maarten
Published: June 2021

The increased awareness of discrepancies between self-reporting outcome measurements and objective outcome measurements within the field of neuromodulation has accelerated the search towards more objective measurements. The aim of this study was to evaluate whether an electronic nose can differentiate between chronic pain patients in whom Spinal Cord Stimulation (SCS) was activated versus deactivated. Twenty-seven patients with Failed Back Surgery Syndrome (FBSS) participated in this prospective pilot study. Volatile organic compounds in exhaled breath were measured with electronic nose technology (Aeonose™) during SCS on and off states. Random forest was used with a leave-10%-out cross-validation method to determine accuracy of discriminating between SCS on and off states. Our random forest showed an accuracy of 0.56, with an area under the curve of 0.62, a sensitivity of 62% (95% CI: 41–79%) and a specificity of 50% (95% CI: 30–70%). Pain intensity scores were significantly different between both SCS states. Our findings indicate that we cannot discriminate between SCS off and on states based on exhaled breath with the Aeonose™ in patients with FBSS. In clinical practice, these findings imply that with a noninvasive electronic nose, exhaled breath cannot be used as an additional marker of the effect of neuromodulation.
Comparing CAM Algorithms for the Identification of Salient Image Features in Iconography Artwork Analysis
Authors: Pinciroli Vago Nicolò Oreste , Milani Federico , Fraternali Piero , da Silva Torres Ricardo
Published: June 2021

Iconography studies the visual content of artworks by considering the themes portrayed in them and their representation. Computer Vision has been used to identify iconographic subjects in paintings and Convolutional Neural Networks enabled the effective classification of characters in Christian art paintings. However, it still has to be demonstrated if the classification results obtained by CNNs rely on the same iconographic properties that human experts exploit when studying iconography and if the architecture of a classifier trained on whole artwork images can be exploited to support the much harder task of object detection. A suitable approach for exposing the process of classification by neural models relies on Class Activation Maps, which emphasize the areas of an image contributing the most to the classification. This work compares state-of-the-art algorithms (CAM, Grad-CAM, Grad-CAM++, and Smooth Grad-CAM++) in terms of their capacity of identifying the iconographic attributes that determine the classification of characters in Christian art paintings. Quantitative and qualitative analyses show that Grad-CAM, Grad-CAM++, and Smooth Grad-CAM++ have similar performances while CAM has lower efficacy. Smooth Grad-CAM++ isolates multiple disconnected image regions that identify small iconographic symbols well. Grad-CAM produces wider and more contiguous areas that cover large iconographic symbols better. The salient image areas computed by the CAM algorithms have been used to estimate object-level bounding boxes and a quantitative analysis shows that the boxes estimated with Grad-CAM reach 55% average IoU, 61% GT-known localization and 31% mAP. The obtained results are a step towards the computer-aided study of the variations of iconographic elements positioning and mutual relations in artworks and open the way to the automatic creation of bounding boxes for training detectors of iconographic symbols in Christian art images.
Particle Distribution and Motion in Six-Stage Centrifugal Pump by Means of Slurry Experiment and CFD-DEM Simulation
Authors: Deng Liwen , Hu Qiong , Chen Jun , Kang Yajuan , Liu Shaojun
Published: June 2021

Six-stage centrifugal pumps are used in deep-sea mining lifting systems and are required to convey slurry containing coarse particles. A six-stage centrifugal pump suitable for operation in a natural mining system was manufactured. High-flow and full-scaled slurry conveying experiments at a 5% and 9% volume concentration of particles was carried out at a large modified test site with artificial nodules. CFD-DEM simulations were carried out to obtain slurry transport characteristic curves, particle transport and distribution characteristics, where the simulation method was validated by the experiment data. A clarified two-stage pump can be used instead of a multi-stage pump for simplified simulation calculations with acceptable accuracy. Local agglomeration of particles caused by backflow was found at the outlet of the diffuser, and such agglomeration decreased with increasing flow rates. It was found that particles are transported non-uniformly, particles transport in diffusers in strands. Particles are transported in a pulse-like mode within the pump, with the latter stage showing similar particle characteristics to those transported in the previous pump stage.
Livelihood Assessment and Occupational Health Hazard of the Ship-Breaking Industry Workers at Chattogram, Bangladesh
Authors: Ahamad Abu Faisal , Schneider Petra , Khanum Romaza , Mozumder Mohammad Mojibul Hoque , Mitu Sabrina Jannat , Shamsuzzaman Md. Mostafa
Published: June 2021

The ship-breaking industry has become a promising sector in Bangladesh by contributing to the country’s primary steel demand as raw material for re-rolling mills, providing livelihood opportunities for the poor. This paper investigates the livelihood index and health hazards of workers engaged in ship-breaking activities at the Bhatiari coast of Chattogram, Bangladesh. Both qualitative and quantitative data were collected through participatory rural assessment (PRA) tools that included 128 individual interviews (II), ten focus group discussions (FGDs), and 15 key informant interviews (KIIs). The workers’ livelihoods revealed that workers lack basic facilities and are exposed to occupational health hazards due to working in a risky environment. Workers of different origins claimed to have 1 to 6 years of work experience and worked 11 to 12 h a day. More than 60% of workers reported being injured or suffering from various physical problems such as blurred vision, abdominal pain, and skin problems. Labor-intensive and unstable occupations, limited access to medical services, poor housing and sanitation, and lack of basic safety requirements increase workers’ plight. Therefore, the study offers advanced protective equipment, better medical facilities, and a safe workplace to improve the workers’ livelihoods.
Optimizing Transformation Frequency of Cryptococcus neoformans and Cryptococcus gattii Using Agrobacterium tumefaciens
Authors: Fu Jianmin , Brockman Nohelli E. , Wickes Brian L.
Published: June 2021

The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell wall damage, and agar concentration. Agar concentration was found to have a significant effect on the transformant number as transformants increased with agar concentration across all four serotypes. When infection time points were tested, higher agar concentrations were found to result in an earlier transfer of the Ti-plasmid to the yeast cell, with the earliest transformant appearing two h after A. tumefaciens contact with yeast cells. These results demonstrate that A. tumefaciens transformation efficiency can be affected by a variety of factors and continued investigation of these factors can lead to improvements in specific A. tumefaciens/fungus transformation systems.
Trunk Surgery as a Tool to Reduce Foliar Symptoms in Diseases of the Esca Complex and Its Influence on Vine Wood Microbiota
Authors: Pacetti Andrea , Moretti Samuele , Pinto Catia , Compant Stéphane , Farine Sibylle , Bertsch Christophe , Mugnai Laura
Published: June 2021

In the last few years, trunk surgery has gained increasing attention as a method to reduce foliar symptoms typical of some of the Esca complex diseases. The technique relies on the mechanical removal of decayed wood by a chainsaw. A study on a 14-year-old Cabernet Sauvignon vineyard was carried out to validate the efficacy of trunk surgery and explore possible explanations behind it. Three levels of treatment were applied to three of the most characteristic symptoms associated with some diseases of the Esca complex, such as leaf stripe symptoms (LS), wilted shoots (WS) and apoplexy (APP). The most promising results were obtained by complete trunk surgery, where the larger decay removal allowed lower symptom re-expression. According to the wood types analyzed (decay, medium and sound wood), different changes in microbiota were observed. Alpha-diversity generally decreased for bacteria and increased for fungi. More specifically, main changes were observed for Fomitiporia mediterranea abundance that decreased considerably after trunk surgery. A possible explanation for LS symptom reduction after trunk surgery could be the microbiota shifting caused by the technique itself affecting a microbic-shared biochemical pathway involved in symptom expression.