bluuebunny/crossref_metadata_2025 huggingface dataset:
Urological Complications Associated With Pyeloureterostomy Without Ipsilateral Nephrectomy in Renal Transplant Recipients
Authors: Neto Hernani M. , Tedesco Silva Junior Helio , Pestana José M. , Foresto Renato D. , Aguiar Wilson F.
Published: January 2022

Background: The implications of ligating the native ureter without ipsilateral nephrectomy after primary kidney transplant pyeloureterostomy (PU) have been described previously.

Methods: This single-center retrospective cohort study including 4,215 kidney transplants performed between February 2010 and December 2014, analyzed urological complications following primary (P-PU) and secondary (S-PU) pyeloureterostomy used to treat urological leaks (UL-PU) and ureteral stenosis (US-PU) without concomitant ipsilateral nephrectomy, in a large cohort of patients.

Results: There were 495 (11.7%) pyeloureterostomy with native ureter ligation without nephrectomy, 409 P-PU (82.6%) and 86 S-PU (17.4%), of which 76 were UL-PU and 10 were US-PU. The median follow-up was 33.8 months. The incidence of native ipsilateral kidney complications requiring nephrectomy was 2.02% (n = 10). Urinary leak was diagnosed in 3.6% of patients after P-UP and 9.2% after UL-PU. Ureteral stenosis was diagnosed in 1.7% of patients after P-UP, 3.9% after UL-PU and 10% after US-PU.

Conclusion: This cohort analysis suggests that native ureter ligation during pyeloureterostomy without native nephrectomy is associated with low incidence of clinically indicated ipsilateral native nephrectomy. Caution and awareness should be emphasized in patients with history of ADPKD and neurogenic augmented bladders.
Adolescents in the Time of COVID: Findings from India and Nepal
Authors: Nagaraj Nitasha , Landry Megan , Hingorani Richa , Luintel Jaya , Vyas Amita
Published: January 2022

Historically, epidemics and humanitarian crises have disproportionately impacted the most vulnerable, impacting mostly girls and women living in low-to-middle income countries. Entrenched inequalities in gender norms and access to education often leave young girls inadequately equipped to effectively protect themselves and their families during an outbreak. There is no exception now as the COVID-19 pandemic has yielded unintended consequences for both adolescent boys and girls. This quantitative research study explores adolescent boys’ and girls’ COVID-19 related perceptions of risk and related behaviors and challenges related to school closures almost one year after the start of the pandemic in India and Nepal. Males were more likely to report completing online/mobile learning and assignments provided by school, whereas females reported a higher percentage of studying with their own books, using other learning resources, or doing nothing while schools have been shut down. Girls were more likely to report wanting to return to school after the pandemic was over. Compared to girls, boys were more likely to report spending more time on household chores than they did before the pandemic. COVID-19 recovery efforts are imperative in alleviating the pandemic’s adverse consequences for adolescents’ education, health well-being, and gender inequities.
Effects of Harvest Stage, Storage, and Preservation Technology on Postharvest Ornamental Value of Cut Peony (Paeonia lactiflora) Flowers
Authors: Sun Jing , Guo Haixia , Tao Jun
Published: January 2022

Herbaceous Peony has high ornamental value but short flowering period, which limits the development of the cut flower industrialization. We studied the effects of different harvest stages, pre-treatment solutions, storage methods, and preservation solutions, including different carbon sources on the ornamental of cut Paeonia lactiflora flowers. The results showed that the “Open-top type” peony had the highest flowering rate and longer vase life harvested at the soft-bud (SB) stage, and the “unfold-petal type” peony collected at the changing-color (CC) stage had the highest flowering rate and the best vase ornamental. In addition, the vase effect of cut peony pre-treated with 8-hydroxyquinoline (8-HQ) and nanosilver (NS) was better than that of the control, and the decline of related protective enzyme activity slowed down in the later stage of storage. The protective enzyme activity of cold storage and low oxygen (CS + LO) treated cut flowers was higher than that of cola storage (CS) treated cut flowers. Apparently, in the fresh-keeping solution containing glucose, sucrose, and trehalose, the flower diameter of cut flowers increased, and the vase life was prolonged, among which the increase of flower diameter treated with glucose was the most significant, and trehalose can delay flowering, increase flowering period, and delay aging.
Aboveground Biomass, Carbon Sequestration, and Yield of Pyrus pyrifolia under the Management of Organic Residues in the Subtropical Ecosystem of Southern Brazil
Authors: da Silva Lucas Jónatan Rodrigues , Souza Tancredo , Laurindo Lídia Klestadt , Nascimento Gislaine dos Santos , de Lucena Edjane Oliveira , Freitas Helena
Published: January 2022

Organic residues management (ORM) alter plant traits and soil properties by changing nutrient and carbon cycling. It is unclear how ORM (mulching, compost, and their combination) applied for 18 months creates a mechanism to promote changes in a P. pyrifolia field. Our aim was to evaluate the influence of ORM on P. pyrifolia nutritional status, plant traits, yield, and carbon sequestration in a 16-year subtropical P. pyrifolia field. For this purpose, we performed an experiment in a randomized block design, using a factorial scheme 2 × 2, with the use of Compost and Mulching (e.g., presence and absence). The highest values of leaves N content, plant height, stem biomass, root biomass, total biomass, yield, and above- and belowground carbon (C) density were found on plots that received compost as the ORM. For soil organic C stock, the highest values were found on plots where mulching was applied. Finally, the highest values of total C density were found on plots that received the combination of Mulching and Compost. Our findings suggest that: (i) the use of Compost is the best alternative to promote leaves N content, plant height, stem dry biomass, root dry biomass, and total dry biomass, plant yield, and above- and belowground C density into a 16-year P. pyrifolia field into subtropical conditions; and (ii) the soil organic C stocks were improved using just the mulching treatment. The results highlight the importance of considering just one organic residue practice based on a sustainable way to improve both plant production and carbon sequestration, no differences were found between the use of compost and the combination of compost and mulching.
Specialty Crop Germplasm and Public Breeding Efforts in the United States
Authors: Orton Thomas , Ayeni Albert
Published: January 2022

The United States Department of Agriculture/Agriculture Research Service/National Germplasm System (USDA/ARS/NGS) plant germplasm collections contain more than 600,000 different accessions of 16,289 species, including virtually all the ethnic and specialty crops of interest to United States (U.S.) agriculture. These and additional collections of plant seeds and asexual propagules are maintained at various laboratories and facilities geographically dispersed in the U.S. including NGS and many public land grant university institutions. The majority of these species fall under the definition of “specialty” crops since their utility is either narrow in scope or has not been fully developed. This paper summarizes the status of “specialty” and ethnic crop species germplasm in the U.S. including where the collections are maintained and how they are being used.
Influence of Slaughter Weight and Sex on Growth Performance, Carcass Characteristics and Ham Traits of Heavy Pigs Fed Ad-Libitum
Authors: Malgwi Isaac Hyeladi , Giannuzzi Diana , Gallo Luigi , Halas Veronika , Carnier Paolo , Schiavon Stefano
Published: January 2022

Slaughter weight (SW) is critical for dry-cured ham production systems with heavy pigs. A total of 159 C21 Goland pigs (gilts and barrows) at 95 ± 9.0 kg body weight (BW) from three batches were used to investigate the impact of ad libitum feeding on SW, growth performance, feed efficiency, and carcass and green ham characteristics. Diets contained 10 MJ/kg of net energy and 7.4 and 6.0 g/kg of SID-lysine. Slaughter weight classes (SWC) included <165, 165–180, 180–110 and >210 kg BW. In each batch, pigs were sacrificed at 230 or 258 d of age. Left hams were scored for round shape, fat cover thickness, marbling, lean colour, bicolour and veining. Data were analyzed with a model considering SWC, sex and SWC × Sex interactions as fixed factors and the batch as a random factor. The linear, quadratic and cubic effects of SWC were tested, but only linear effects were found. Results showed that pigs with greater SWC had greater average daily gain and feed consumption, with similar feed efficiency and better ham quality traits: greater ham weight, muscularity, and fat coveringin correspondence of semimembranosus muscle. Barrows were heavier and produced hams with slightly better characteristics than gilts.
Quercetin Improves Pulmonary Function and Prevents Emphysema Caused by Exposure to Cigarette Smoke in Male Mice
Authors: Araújo Natália Pereira da Silva , de Matos Natália Alves , Oliveira Michel , de Souza Ana Beatriz Farias , Castro Thalles de Freitas , Machado-Júnior Pedro Alves , de Souza Débora Maria Soares , Talvani André , Cangussú Sílvia Dantas , de Menezes Rodrigo Cunha Alvim , Bezerra Frank Silva
Published: January 2022

Chronic obstructive pulmonary disease (COPD) is the major cause of morbidity and mortality worldwide, and cigarette smoke is a key factor in the development of COPD. Thus, the development of effective therapies to prevent the advancement of COPD has become increasingly essential. We hypothesized that quercetin protects lungs in mice exposed to long-term cigarette smoke. Thirty-five C57BL/6 mice were exposed to cigarette smoke (12 cigarettes per day) for 60 days and pretreated with 10 mg/kg/day of quercetin via orogastric gavage. After the experimental protocol, the animals were euthanized and samples were collected for histopathological, antioxidant defense, oxidative stress and inflammatory analysis. The animals exposed to cigarette smoke showed an increase in respiratory rate and hematological parameters, cell influx into the airways, oxidative damage and inflammatory mediators, besides presenting with alterations in the pulmonary histoarchitecture. The animals receiving 10 mg/kg/day of quercetin that were exposed to cigarette smoke presented a reduction in cellular influx, less oxidative damage, reduction in cytokine levels, improvement in the histological pattern and improvement in pulmonary emphysema compared to the group that was only exposed to cigarette smoke. These results suggest that quercetin may be an agent in preventing pulmonary emphysema induced by cigarette smoke.
Numerical Investigation of the Temporal Contrast in ps-OPCPA with Compact Double BBO Arrangement
Authors: Chen Haidong , Hu Jiabing , Wang Xinliang , Bai Peile , Chen Xun , Yang Xihang , Wu Fenxiang , Zhang Zongxin , Yang Xiaojun , Gui Jiayan , Qian Jiayi , Liu Yanqi , Xu Yi , Leng Yuxin
Published: January 2022

The picosecond optical parametric chirped pulse amplifier (ps-OPCPA) with double BBO arrangement can support the ultrabroad spectrum even under a relatively long pump pulse duration (∼100 ps). In this work, five-wave-coupled equations taking into consideration different phase matching conditions between the parametric superfluorescence (PSF) and the signal are proposed to investigate the temporal contrast in ps-OPCPA schemes. Both the temporal contrast and the amplified spectrum are numerically analyzed in double BBO arrangements with four phase matching conditions. Numerical results show that the high temporal contrast and ultrabroad spectrum can be simultaneously realized by choosing the proper phase matching geometry in a double BBO arrangement. The numerical investigation here relaxes the requirement of very short pump pulses in ps-OPCPA, which can provide beneficial guidance for the design and construction of ps-OPCPA.
Differential Immunostimulatory Effects of Lipoteichoic Acids Isolated from Four Strains of Lactiplantibacillus plantarum
Authors: Jung Bong-Jun , Kim Hangeun , Chung Dae-Kyun
Published: January 2022

The intestinal health and immune modulatory effects of probiotics are well known. As with live bacteria, several studies demonstrating the ability of dead cells to improve gut health and immunity have suggested varying potentials for microbes to affect the human gut. The effect of dead microbes most likely derives from the cell wall of the microorganism. In this study, the functionality of lipoteichoic acid (LTA), a cell wall component, isolated from four stains of Lactiplantibacillus plantarum, K8, K88, K5-5, and K55-5, and the relationship between LTAs and their receptors were investigated. The four strains of L. plantarum have different LTA structures, which contributed to different immune activities in the immune cells. We confirmed that the different binding abilities with the host cell surface receptors, along with the differences in signal pathway, were due to the structural differences of the LTAs. LTA is an important postbiotic that induces various immunomodulatory actions.
Future Changes in High and Low Flows under the Impacts of Climate and Land Use Changes in the Jiulong River Basin of Southeast China
Authors: Yang Shuyu , Yang Dawen , Zhao Baoxu , Ma Teng , Lu Weiwei , Santisirisomboon Jerasorn
Published: January 2022

Climate change and human activities have profoundly affected the world with extreme precipitation, heat waves, water scarcity, frequent floods and intense droughts. It is acknowledged that climate change will persist and perhaps intensify in the future, and it is thus meaningful to explore the quantitative impacts of these changes on hydrological regimes. The Jiulong River basin serves as an important watershed on the southeast coast of China. However, future hydrological changes under the combined impacts of climate change and land use change have been barely investigated. In this study, the climate outputs from five general circulation models (GCMs) under the Coupled Model Intercomparison Project Phase 6 (CMIP6) were corrected and spatially downscaled by a statistical downscaling method combining quantile mapping and machine learning. The future high-resolution land use maps were projected by the CA–Markov model with land use changes from the Land-Use Harmonization 2 (LUH2) as constraints. The future dynamic vegetation process was projected by the Biome-GBC model, and then, the future hydrological process under four representative concentration pathways and shared socioeconomic pathways (RCP–SSP) combined scenarios was simulated by a distributed hydrological model. Based on the copula method, the flood frequency and corresponding return periods were derived. The results demonstrated that future precipitation and air temperature would continue to rise, and future land use changes would have different developing pathways determined by the designs in various SSP–RCPs. Under the combined impacts of climate and land use change, the total available water resources will increase due to increasing precipitation, and the high flow and low flow will both increase at three stations under the four SSP–RCPs. The annual 1-day maximum discharge is projected to increase by 67–133% in the last decade of the 21st century, and the annual 7-day minimum discharge is projected to increase by 19–39%. The flood frequency analysis showed that the Jiulong River basin would face more frequent floods in the future. By the end of the 21st century, the station-average frequency of a historical 100-year flood will increase by 122% under the most optimistic scenario (SSP126) and increase by 213% under the scenario of greatest regional rivalry (SSP370). We demonstrated that climate change would be the major cause for the increase in future high flows and that land use change would dominate future changes in low flows. Finally, we recommend integrated and sustainable water management systems to tackle future challenges in this coastal basin.
Revisiting Total Particle Number Measurements for Vehicle Exhaust Regulations
Authors: Giechaskiel Barouch , Melas Anastasios , Martini Giorgio , Dilara Panagiota , Ntziachristos Leonidas
Published: January 2022

Road transport significantly contributes to air pollution in cities. Emission regulations have led to significantly reduced emissions in modern vehicles. Particle emissions are controlled by a particulate matter (PM) mass and a solid particle number (SPN) limit. There are concerns that the SPN limit does not effectively control all relevant particulate species and there are instances of semi-volatile particle emissions that are order of magnitudes higher than the SPN emission levels. This overview discusses whether a new metric (total particles, i.e., solids and volatiles) should be introduced for the effective regulation of vehicle emissions. Initially, it summarizes recent findings on the contribution of road transport to particle number concentration levels in cities. Then, both solid and total particle emission levels from modern vehicles are presented and the adverse health effects of solid and volatile particles are briefly discussed. Finally, the open issues regarding an appropriate methodology (sampling and instrumentation) in order to achieve representative and reproducible results are summarized. The main finding of this overview is that, even though total particle sampling and quantification is feasible, details for its realization in a regulatory context are lacking. It is important to define the methodology details (sampling and dilution, measurement instrumentation, relevant sizes, etc.) and conduct inter-laboratory exercises to determine the reproducibility of a proposed method. It is also necessary to monitor the vehicle emissions according to the new method to understand current and possible future levels. With better understanding of the instances of formation of nucleation mode particles it will be possible to identify its culprits (e.g., fuel, lubricant, combustion, or aftertreatment operation). Then the appropriate solutions can be enforced and the right decisions can be taken on the need for new regulatory initiatives, for example the addition of total particles in the tailpipe, decrease of specific organic precursors, better control of inorganic precursors (e.g., NH3, SOx), or revision of fuel and lubricant specifications.
Identification and Predictive Value of Risk Factors for Mortality Due to Listeria monocytogenes Infection: Use of Machine Learning with a Nationwide Administrative Data Set
Authors: Garcia-Carretero Rafael , Roncal-Gomez Julia , Rodriguez-Manzano Pilar , Vazquez-Gomez Oscar
Published: January 2022

We used machine-learning algorithms to evaluate demographic and clinical data in an administrative data set to identify relevant predictors of mortality due to Listeria monocytogenes infection. We used the Spanish Minimum Basic Data Set at Hospitalization (MBDS-H) to estimate the impacts of several predictors on mortality. The MBDS-H is a mandatory registry of clinical discharge reports. Data were coded with International Classification of Diseases, either Ninth or Tenth Revisions, codes. Diagnoses and clinical conditions were defined using recorded data from these codes or a combination of them. We used two different statistical approaches to produce two predictive models. The first was logistic regression, a classic statistical approach that uses data science to preprocess data and measure performance. The second was a random forest algorithm, a strategy based on machine learning and feature selection. We compared the performance of the two models using predictive accuracy and the area under the curve. Between 2001 and 2016, a total of 5603 hospitalized patients were identified as having any clinical form of listeriosis. Most patients were adults (94.9%). Among all hospitalized individuals, there were 2318 women (41.4%). We recorded 301 pregnant women and 287 newborns with listeriosis. The mortality rate was 0.13 patients per 100,000 population. The performance of the model produced by logistic regression after intense preprocessing was similar to that of the model produced by the random forest algorithm. Predictive accuracy was 0.83, and the area under the receiver operating characteristic curve was 0.74 in both models. Sepsis, age, and malignancy were the most relevant features related to mortality. Our combined use of data science, preprocessing, conventional statistics, and machine learning provides insights into mortality due to Listeria-related infection. These methods are not mutually exclusive. The combined use of several methods would allow researchers to better explain results and understand data related to Listeria monocytogenes infection.
Noise Power Minimization in CMOS Brain-Chip Interfaces
Authors: Stevenazzi Lorenzo , Baschirotto Andrea , Zanotto Giorgio , Vallicelli Elia Arturo , De Matteis Marcello
Published: January 2022

This paper presents specific noise minimization strategies to be adopted in silicon–cell interfaces. For this objective, a complete and general model for the analog processing of the signal coming from cell–silicon junctions is presented. This model will then be described at the level of the single stages and of the fundamental parameters that characterize them (bandwidth, gain and noise). Thanks to a few design equations, it will therefore be possible to simulate the behavior of a time-division multiplexed acquisition channel, including the most relevant parameters for signal processing, such as amplification (or power of the analog signal) and noise. This model has the undoubted advantage of being particularly simple to simulate and implement, while maintaining high accuracy in estimating the signal quality (i.e., the signal-to-noise ratio, SNR). Thanks to the simulation results of the model, it will be possible to set an optimal operating point for the front-end to minimize the artifacts introduced by the time-division multiplexing (TDM) scheme and to maximize the SNR at the a-to-d converter input. The proposed results provide an SNR of 12 dB at 10 µVRMS of noise power and 50 µVRMS of signal power (both evaluated at input of the analog front-end, AFE). This is particularly relevant for cell–silicon junctions because it demonstrates that it is possible to detect weak extracellular events (of the order of few µVRMS) without necessarily increasing the total amplification of the front-end (and, therefore, as a first approximation, the dissipated electrical power), while adopting a specific gain distribution through the acquisition chain.
Selective Isolation of Liver-Derived Extracellular Vesicles Redefines Performance of miRNA Biomarkers for Non-Alcoholic Fatty Liver Disease
Authors: Newman Lauren A. , Useckaite Zivile , Johnson Jillian , Sorich Michael J. , Hopkins Ashley M. , Rowland Andrew
Published: January 2022

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. Definitive diagnosis of the progressive form, non-alcoholic steatohepatitis (NASH), requires liver biopsy, which is highly invasive and unsuited to early disease or tracking changes. Inadequate performance of current minimally invasive tools is a critical barrier to managing NAFLD burden. Altered circulating miRNA profiles show potential for minimally invasive tracking of NAFLD. The selective isolation of the circulating extracellular vesicle subset that originates from hepatocytes presents an important opportunity for improving the performance of miRNA biomarkers of liver disease. The expressions of miR-122, -192, and -128-3p were quantified in total cell-free RNA, global EVs, and liver-specific EVs from control, NAFL, and NASH subjects. In ASGR1+ EVs, each miR biomarker trended positively with disease severity and expression was significantly higher in NASH subjects compared with controls. The c-statistic defining the performance of ASGR1+ EV derived miRNAs was invariably >0.78. This trend was not observed in the alternative sources. This study demonstrates the capacity for liver-specific isolation to transform the performance of EV-derived miRNA biomarkers for NAFLD, robustly distinguishing patients with NAFL and NASH.
Biologics in Severe Eosinophilic Asthma: Three-Year Follow-Up in a SANI Single Center
Authors: Solidoro Paolo , Nicola Stefania , Ridolfi Irene , Canonica Giorgio Walter , Blasi Francesco , Paggiaro Pierluigi , Heffler Enrico , Bagnasco Diego , Patrucco Filippo , Ribolla Fulvia , Bucca Caterina , Rolla Giovanni , Albera Carlo , Brussino Luisa
Published: January 2022

Introduction: Biologic drugs have dramatically improved severe eosinophilic asthma (SEA) outcomes. Our aim was to evaluate the long-term efficacy of biological therapy in SEA in a real-life setting and to identify the predictors for switching to another biological drug in patients with poor asthma control. The outcomes for efficacy were decreased annual exacerbations (AE) and improved asthma control test (ACT). Methods: In 90 SEA patients being treated with a biological drug, clinical examination, ACT, blood eosinophils count and spirometry were assessed before (T0) and after 6 (T1), 12 (T2), 24 (T3) and 36 (T4) months from the start of biological therapy. Patients were considered responders (R) or non-responders (NR) to biologics depending on whether or not they had less than two AE and a 20% increase in the ACT after 12 months of treatment. Results: 75% of the patients were R, 25% NR. In R patients, biological therapy add-on was followed by significant improvement in AE and ACT throughout the whole follow-up period. The percentage of patients on oral corticosteroids (OCS) dropped from 40% to 12%. By contrast, the NR patients were shifted to another biological drug after 12 months of therapy, as they still had high AE and nearly unchanged ACT; 40% of them still needed OCS treatment. The predictors of switching to another biological drug were three or more AE, ACT below 17, nasal polyposis and former smoking (p < 0.05). In NR, the shift to another biological drug was followed by a significant decrease in AE and an increase in the ACT. Discussion: This real-life study confirms the long-term efficacy of biologics in most SEA patients and indicates that even in non-responders to a first biological drug, it is worth trying a second one. It is hoped that the availability of additional biologics with different targets will help improve the personalization of SEA therapy.
Update on Domestic Violence and Traumatic Brain Injury: A Narrative Review
Authors: Costello Kellianne , Greenwald Brian D.
Published: January 2022

Research on traumatic brain injury (TBI) as a result of domestic violence has greatly increased in the past decade, with publications addressing the prevalence, diagnosis, evaluation, and treatment. Although TBI due to domestic violence has recently been found to occur quite frequently, it was not widely understood until the 1990s. Individuals who suffer from domestic violence TBI often experience sequelae such as decreased cognitive functioning, memory loss, and PTSD. The goal of this article is to increase awareness about TBI secondary to domestic violence, with the intent that it will highlight areas for future research on the diagnosis, evaluation, and treatment of TBI in this population. The articles in this study were first found using the search terms traumatic brain injury and domestic violence. Although, in recent years, there has been a significant increase in research on TBI due to domestic violence, the overall conclusion of this review article is that there is still a need for future research in many areas including the effects on minority populations, the effects of COVID-19, and improvements of screening tools.
Neurobiological Responses towards Stimuli Depicting Aggressive Interactions in Delinquent Young Adults and Controls: No Relation to Reactive and Proactive Aggression
Authors: ter Harmsel Janna F. , van Dongen Josanne D. M. , Zijlmans Josjan , van der Pol Thimo M. , Marhe Reshmi , Popma Arne
Published: January 2022

Neurobiological measures underlying aggressive behavior have gained attention due to their potential to inform risk assessment and treatment interventions. Aberrations in responsivity of the autonomic nervous system and electrophysiological responses to arousal-inducing stimuli have been related to emotional dysregulation and aggressive behavior. However, studies have often been performed in community samples, using tasks that induce arousal but not specifically depict aggression. In this study, we examined differences in psychophysiological (i.e., heart rate, respiratory sinus arrhythmia, skin conductance level) and electrophysiological responses (i.e., P3, late positive potential, mu suppression) to aggressive versus neutral scenes in a sample of 118 delinquent young adults and 25 controls (all male, aged 18–27). With respect to group differences, we only found significant higher SCL reactivity during the task in the delinquent group compared to controls, but this was irrespective of condition (aggressive and neutral interactions). Within the delinquent group, we also examined associations between the neurobiological measures and reactive and proactive aggression. No significant associations were found. Therefore, although we found some indication of emotional dysregulation in these delinquent young adults, future studies should further elucidate the neurobiological mechanisms underlying emotional dysregulation in relation to different types of aggression.
Impact of Pre-Analytical and Analytical Variables Associated with Sample Preparation on Flow Cytometric Stainings Obtained with EuroFlow Panels
Authors: Sędek Łukasz , Flores-Montero Juan , van der Sluijs Alita , Kulis Jan , te Marvelde Jeroen , Philippé Jan , Böttcher Sebastian , Bitter Marieke , Caetano Joana , van der Velden Vincent H. J. , Sonneveld Edwin , Buracchi Chiara , Santos Ana Helena , Lima Margarida , Szczepański Tomasz , van Dongen Jacques J. M. , Orfao Alberto
Published: January 2022

Objective interpretation of FC results may still be hampered by limited technical standardization. The EuroFlow consortium conducted a series of experiments to determine the impact of different variables on the relative distribution and the median fluorescence intensity (MFI) of markers stained on different cell populations, from both healthy donors and patients’ samples with distinct hematological malignancies. The use of different anticoagulants; the time interval between sample collection, preparation, and acquisition; pH of washing buffers; and the use of cell surface membrane-only (SM) vs. cell surface plus intracytoplasmic (SM+CY) staining protocols, were evaluated. Our results showed that only monocytes were represented at higher percentages in EDTA- vs. heparin-anticoagulated samples. Application of SM or SM+CY protocols resulted in slight differences in the percentage of neutrophils and debris determined only with particular antibody combinations. In turn, storage of samples for 24 h at RT was associated with greater percentage of debris and cell doublets when the plasma cell disorder panel was used. Furthermore, 24 h storage of stained cells at RT was selectively detrimental for MFI levels of CD19 and CD45 on mature B- and T-cells (but not on leukemic blasts, clonal B- and plasma cells, neutrophils, and NK cells). The obtained results showed that the variables evaluated might need to be tailored for sample and cell type(s) as well as to the specific markers compared; however, defining of well-balanced boundaries for storage time, staining-to-acquisition delay, and pH of washing buffer would be a valid recommendation for most applications and circumstances described herein.
MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules
Authors: Biernatowska Agnieszka , Wójtowicz Karolina , Trombik Tomasz , Sikorski Aleksander F. , Czogalla Aleksander
Published: January 2022

MPP1 (membrane palmitoylated protein 1) belongs to the MAGUK (membrane-associated guanylate kinase homologs) scaffolding protein family. These proteins organize molecules into complexes, thereby maintaining the structural heterogeneity of the plasma membrane (PM). Our previous results indicated that direct, high-affinity interactions between MPP1 and flotillins (raft marker proteins) display dominant PM-modulating capacity in erythroid cells. In this study, with high-resolution structured illuminated imaging, we investigated how these complexes are organized within erythroid cells on the nanometer scale. Furthermore, using other spectroscopic techniques, namely fluorescence recovery after photobleaching (FRAP) and spot-variation fluorescence correlation spectroscopy (svFCS), we revealed that MPP1 acts as a key raft-capturing molecule, regulating temporal immobilization of flotillin-based nanoclusters, and controls local concentration and confinement of sphingomyelin and Thy-1 in raft nanodomains. Our data enabled us to uncover molecular principles governing the key involvement of MPP1-flotillin complexes in the dynamic nanoscale organization of PM of erythroid cells.
LbCas12a-D156R Efficiently Edits LOB1 Effector Binding Elements to Generate Canker-Resistant Citrus Plants
Authors: Jia Hongge , Wang Yuanchun , Su Hang , Huang Xiaoen , Wang Nian
Published: January 2022

Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is an economically important disease in most citrus production regions worldwide. Xcc secretes a transcriptional activator like effector (TALE) PthA4 to bind to the effector binding elements (EBEs) in the promoter region of canker susceptibility gene LOB1 to activate its expression, which in turn causes canker symptoms. Editing the EBE region with Cas9/gRNA has been used to generate canker resistant citrus plants. However, most of the EBE-edited lines generated contain indels of 1–2 bp, which has higher possibility to be overcome by PthA4 adaptation. The adaptation capacity of TALEs inversely correlates with the number of mismatches with the EBE. LbCas12a/crRNA is known to generate longer deletion than Cas9. In this study, we used a temperature-tolerant and more efficient LbCas12a variant (ttLbCas12a), harboring the single substitution D156R, to modify the EBE region of LOB1. We first constructed GFP-p1380N-ttLbCas12a:LOBP, which was shown to be functional via Xcc-facilitated agroinfiltration in Pummelo (Citrus maxima) leaves. Subsequently, we stably expressed ttLbCas12a:LOBP in Pummelo. Eight transgenic lines were generated, with seven lines showing 100% mutations of the EBE, among which one line is homozygous. The EBE-edited lines had the ttLbCas12a-mediated deletions of up to 10 bp. Importantly, the seven lines were canker resistant and no off-targets were detected. In summary, ttLbCas12a can be used to efficiently generate biallelic/homozygous citrus mutant lines with short deletions, thus providing a useful tool for the functional study and breeding of citrus.
Discovery of a Novel Aminocyclopropenone Compound That Inhibits BRD4-Driven Nucleoporin NUP210 Expression and Attenuates Colorectal Cancer Growth
Authors: Kondo Hiroya , Mishiro Kenji , Iwashima Yuki , Qiu Yujia , Kobayashi Akiko , Lim Keesiang , Domoto Takahiro , Minamoto Toshinari , Ogawa Kazuma , Kunishima Munetaka , Hazawa Masaharu , Wong Richard W.
Published: January 2022

Epigenetic deregulation plays an essential role in colorectal cancer progression. Bromodomains are epigenetic “readers” of histone acetylation. Bromodomain-containing protein 4 (BRD4) plays a pivotal role in transcriptional regulation and is a feasible drug target in cancer cells. Disease-specific elevation of nucleoporin, a component of the nuclear pore complex (NPC), is a determinant of cancer malignancy, but BRD4-driven changes of NPC composition remain poorly understood. Here, we developed novel aminocyclopropenones and investigated their biological effects on cancer cell growth and BRD4 functions. Among 21 compounds developed here, we identified aminocyclopropenone 1n (ACP-1n) with the strongest inhibitory effects on the growth of the cancer cell line HCT116. ACP-1n blocked BRD4 functions by preventing its phase separation ability both in vitro and in vivo, attenuating the expression levels of BRD4-driven MYC. Notably, ACP-1n significantly reduced the nuclear size with concomitant suppression of the level of the NPC protein nucleoporin NUP210. Furthermore, NUP210 is in a BRD4-dependent manner and silencing of NUP210 was sufficient to decrease nucleus size and cellular growth. In conclusion, our findings highlighted an aminocyclopropenone compound as a novel therapeutic drug blocking BRD4 assembly, thereby preventing BRD4-driven oncogenic functions in cancer cells. This study facilitates the development of the next generation of effective and potent inhibitors of epigenetic bromodomains and extra-terminal (BET) protein family.
Inhibition of Hedgehog Delays Liver Regeneration through Disrupting the Cell Cycle
Authors: Tao Jiawang , Chen Yan , Zhuang Yuanqi , Wei Ruzhi , Getachew Anteneh , Pan Tingcai , Yang Fan , Li Yinxiong
Published: January 2022

Liver regeneration is a complicated biological process orchestrated by various liver resident cells. Hepatic cell proliferation and reconstruction of the hepatic architecture involve multiple signaling pathways. It has been reported that the Hh signal is involved in liver regeneration. However, the signal transduction pathways and cell types involved are ill studied. This study aimed to investigate hedgehog signal response cell types and the specific molecular mechanism involved in the process of liver regeneration. Partial hepatectomy (PH) of 70% was performed on ICR (Institute of Cancer Research) mice to study the process of liver regeneration. We found that the hedgehog signal was activated significantly after PH, including hedgehog ligands, receptors and intracellular signaling molecules. Ligand signals were mainly expressed in bile duct cells and non-parenchymal hepatic cells, while receptors were expressed in hepatocytes and some non-parenchymal cells. Inhibition of the hedgehog signal treated with vismodegib reduced the liver regeneration rate after partial hepatectomy, including inhibition of hepatic cell proliferation by decreasing Cyclin D expression and disturbing the cell cycle through the accumulation of Cyclin B. The current study reveals the important role of the hedgehog signal and its participation in the regulation of hepatic cell proliferation and the cell cycle during liver regeneration. It provides new insight into the recovery of the liver after liver resection.
Case Report of Small Cell Carcinoma of the Ovary, Hypercalcemic Type (Ovarian Rhabdoid Tumor) with SMARCB1 Mutation: A Literature Review of a Rare and Aggressive Condition
Authors: Simões Maria Fernanda Evangelista , da Costa Alexandre André Balieiro Anastácio , Silva Tullio Novaes , Fernandes Lizieux , Bovolim Graziele , Torrezan Giovana Tardin , Carraro Dirce Maria , Baiocchi Glauco , Menezes Ademir Narcizo Oliveira , Santana Dos Santos Elizabeth , De Brot Louise
Published: January 2022

Small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) is a rare and aggressive condition that is associated with the SMARCA4 mutation and has a dismal prognosis. It is generally diagnosed in young women. Here, we report a case of a young woman with SCCOHT harboring a rare molecular finding with a highly aggressive biological behavior. The patient had a somatic SMARCB1 mutation instead of an expected SMARCA4 alteration. Even though the patient was treated with high-dose chemotherapy followed by stem cell transplantation, she evolved with disease progression and died 11 months after her first symptoms appeared. We present a literature review of this rare disease and discuss the findings in the present patient in comparison to expected molecular alterations and options for SCCOHT treatment.
Who Is Responsible for Students’ Challenging Behaviour? A Study of the Causal Attributions of Teachers to Challenging Behaviour in Primary Schools in West Bengal, India
Authors: Patnaik Susmita , Sharma Umesh , Subban Pearl
Published: January 2022

The aim of the study was to understand the causal attributions of teachers to challenging behaviours in primary classrooms in West Bengal, India. Data from 21 teachers from government and private primary schools were collected using semistructured interviews. The study investigated the types of challenging behaviours as perceived by teachers, their causal attributions, and the strategies suggested by the teachers for managing them. The participants described challenging behaviour broadly, and it was divided into four categories: aggression, disruption, talking, and noncompliance. They reported the causes of challenging behaviours in five broad categories: home- and parent-related causes (family violence, busy parents); social causes (socioeconomic conditions); student-related causes (disabilities); school- and teacher-related causes (large class sizes); and government- and policy-related causes (banning corporal punishment). The teachers predominantly recommended employing proactive strategies, such as improving teaching strategies, collaborating with parents, and building relationships with students. A small group of teachers recommended using reactive (e.g., discipline and threats) strategies to manage challenging behaviours in their classrooms.
Detection of Internal Defects in Concrete and Evaluation of a Healthy Part of Concrete by Noncontact Acoustic Inspection Using Normalized Spectral Entropy and Normalized SSE
Authors: Sugimoto Kazuko , Sugimoto Tsuneyoshi
Published: January 2022

Non-destructive testing, with non-contact from a remote location, to detect and visualize internal defects in composite materials such as a concrete is desired. Therefore, a noncontact acoustic inspection method has been studied. In this method, the measurement surface is forced to vibrate by powerful aerial sound waves from a remote sound source, and the vibration state is measured by a laser Doppler vibrometer. The distribution of acoustic feature quantities (spectral entropy and vibrational energy ratio) is analyzed to statistically identify and evaluate healthy parts of concrete. If healthy parts in the measuring plane can be identified, the other part is considered to be internal defects or an abnormal measurement point. As a result, internal defects are detected. Spectral entropy (SE) was used to distinguish between defective parts and healthy parts. Furthermore, in order to distinguish between the resonance of a laser head and the resonance of the defective part of the concrete, spatial spectral entropy (SSE) was also used. SSE is an extension of the concept of SE to a two-dimensional measuring space. That is, based on the concept of SE, SSE is calculated, at each frequency, for spatial distribution of vibration velocity spectrum in the measuring plane. However, these two entropy values were used in unnormalized expressions. Therefore, although relative evaluation within the same measurement surface was possible, there was the issue that changes in the entropy value could not be evaluated in a unified manner in measurements under different conditions and environments. Therefore, this study verified whether it is possible to perform a unified evaluation for different defective parts of concrete specimen by using normalized SE and normalized SSE. From the experimental results using cavity defects and peeling defects, the detection and visualization of internal defects in concrete can be effectively carried out by the following two analysis methods. The first is using both the normalized SE and the evaluation of a healthy part of concrete. The second is the normalized SSE analysis that detects resonance frequency band of internal defects.
Standing Wave Pattern and Distribution of Currents in Resonator Arrays for Wireless Power Transfer
Authors: Simonazzi Mattia , Reggiani Ugo , Sandrolini Leonardo
Published: January 2022

The possibility of increasing the transmission efficiency in mid-range wireless power transfer (WPT) applications can be achieved by inserting resonant relay coils between the transmitting and receiving sides of the device, forming an array of magnetically coupled resonant circuits, over which a receiver can be placed. This is a very cheap solution for improving the performance of the WPT apparatus, even if the complexity of the system increases, requiring a complete and detailed investigation for a smart design and control of the apparatus. The presented study investigates the current distribution in the coils of the array, which revealed strong peaks in magnitude depending on the load and receiver position. The analysis is carried out with the transmission line (TL) theory and it is performed for different positions of the receiver, as well as for different load conditions. Furthermore, a real application is considered and discussed, which includes the presence of a power converter as power supply and a battery charging system as load. Each resonant circuit resonates at 150 kHz and the whole apparatus is capable to transmit power up to 1 kW with an efficiency around 70%. The theoretical results have been validated with experimental measurements.
Experimental Lognormal Modeling of Harmonics Power of Switched-Mode Power Supplies
Authors: Bykhovsky Dima
Published: January 2022

Switched-mode power supplies (SMPSs) are an important component in many electrical systems. As a highly non-linear device, an unavoidable side-effect of SMPS operation is its high harmonics power. One of the ways to model the harmonic power consumption profile is in terms of a random process. This paper addresses random process modeling with a corresponding probability density function (PDF), auto-covariance function (ACF) and spectral coherence. The consumed harmonics power was evaluated under different load conditions and is based on experimental results of current consumption from SMPSs. The analysis shows that harmonics power may be modeled by a lognormal distribution that is time-domain uncorrelated, and that has spectral-domain correlation modeled by a Gaussian radial basis function. Extensive discussion on the modeling results is also provided. Moreover, random simulation approach based on the modeling results was proposed.
Alternative Fuel Generation from Dangerous Solid Waste in a Protected Environmental Area
Authors: Mora Pedro , Barettino Daniel , Ponce Antonio , Sánchez-Martín Laura , Llamas Bernardo
Published: January 2022

The present investigation project aims to evaluate the extraction of contaminant material from two settling ponds to be used as alternative fuel in two cement plants. The extraction is carried out through mechanical means, and after that extraction, two options are compared: energy recovery and incineration. Through energy recovery, a potentially contaminated area is decontaminated and its waste is used; its high calorific value makes this option a viable one. The waste extraction is carried out through mechanical means due to the high density and viscosity of the waste. Because of these characteristics, the waste undergoes an on-site security adaptation to stabilize it, reduce declivity risk and make it suitable to be handled and moved. The second treatment is carried out in external installations where the final product is obtained (alternative fuel), which is to be used at industrial kilns. The entire described process shows a difference on the consumed energy of 6060.42 kWh/twaste between the two options under study: waste incineration and energy recovery. In addition, it also reduces CO2 emissions on 2.178 tCO2/twaste.