bluuebunny/crossref_metadata_2025 huggingface dataset:
Thermal Time and Cardinal Temperatures for Germination of Cedrela odorata L.
Authors: Sampayo-Maldonado Salvador , Ordoñez-Salanueva Cesar A. , Mattana Efisio , Ulian Tiziana , Way Michael , Castillo-Lorenzo Elena , Dávila-Aranda Patricia D. , Lira-Saade Rafael , Téllez-Valdéz Oswaldo , Rodriguez-Arevalo Norma I. , Flores-Ortíz Cesar M.
Published: September 2019

Thermal time models are useful to determine the thermal and temporal requirements for seed germination. This information may be used as a criterion for species distribution in projected scenarios of climate change, especially in threatened species like red cedar. The objectives of this work were to determine the cardinal temperatures and thermal time for seeds of Cedrela odorata and to predict the effect of increasing temperature in two scenarios of climate change. Seeds were placed in germination chambers at constant temperatures ranging from 5 ± 2 to 45 ± 2 °C. Germination rate was analyzed in order to calculate cardinal temperatures and thermal time. The time required for germination of 50% of population was estimated for the current climate, as well as under the A2 and B2 scenarios for the year 2050. The results showed that base, optimal and maximal temperatures were −0.5 ± 0.09, 38 ± 1.6 and 53.3 ± 2.1 °C, respectively. Thermal time (θ1(50)) was 132.74 ± 2.60 °Cd, which in the current climate scenario accumulates after 5.5 days. Under the A2 scenario using the English model, this time is shortened to 4.5 days, while under scenario B2, the time is only 10 hours shorter than the current scenario. Under the German model, the accumulation of thermal time occurs 10 and 6.5 hours sooner than in the current climate under the A2 and B2 models, respectively. The seeds showed a wide range of temperatures for germination, and according to the climate change scenarios, the thermal time accumulates over a shorter period, accelerating the germination of seeds in the understory. This is the first report of a threshold model for C. odorata, one of the most important forest species in tropical environments.
Volatile Profile and Physico-Chemical Analysis of Acacia Honey for Geographical Origin and Nutritional Value Determination
Authors: Mădaş Niculina M. , Mărghitaş Liviu A. , Dezmirean Daniel S. , Bonta Victorita , Bobiş Otilia , Fauconnier Marie-Laure , Francis Frédéric , Haubruge Eric , Nguyen Kim B.
Published: September 2019

Honey composition and color depend greatly on the botanical and geographical origin. Water content, water activity and color of 50 declared acacia samples, collected from three different geographical zones of Romania, together with chromatographic determination of sugar spectrum were analyzed. A number of 79 volatile compounds from the classes of: Alcohols, aldehydes, esters, ketones, sulphur compounds, aliphatic hydrocarbons, nitrogen compounds, carboxylic acids, aromatic acids and ethers were identified by solid-phase micro-extraction and gas-chromatography mass spectrometry. The overall volatile profile and sugar spectrum of the investigated honey samples allow the differentiation of geographical origin for the acacia honey samples subjected to analysis. The statistical models of the chromatic determination, physicochemical parameters and volatile profile was optimal to characterize the honey samples and group them into three geographical origins, even they belong to the same botanical origin.
Inferring Opinions and Behavioral Characteristics of Gay Men with Large Scale Multilingual Text from Blued
Authors: Huang Ge , Cai Mengsi , Lu Xin
Published: September 2019

Gay men in many countries are increasingly using geosocial networking applications (GSN apps), thus offering new opportunities for understanding them. This paper provides a comprehensive content analysis of posts and opinions on Blued, the world’s largest gay social networking dating app, to infer and compare opinions and behavioral characteristics of gay men in different countries. Machine learning and linguistic programming approaches were used to extract themes and analyze sentiments of posts. The results show that the majority of posts are related to daily life activities, and less are related to sensitive topics. While most posts are positive or neutral, negative emotions, including anxiety, anger, and sadness, are mainly distributed in posts related to self-identification and sexual behaviors in China and to relationships in other countries. Voting items indicate that only 50.52% of the participants will take regular HIV tests while 50.2% would have casual sex when they are single. Additionally, 35.8% of the participants may try drugs when invited by friends. Our findings suggest an opportunity and necessity for researchers and public health practitioners to use open source data on GSN apps and other social medias to inform HIV interventions and to promote social inclusion for sexual minorities.
Local Residents’ Perceptions for Ecosystem Services: A Case Study of Fenghe River Watershed
Authors: Zhang Hongjuan , Pang Qian , Long Huan , Zhu Haochen , Gao Xin , Li Xiuqing , Jiang Xiaohui , Liu Kang
Published: September 2019

To make environmental management decisions more executive and targeted, it is essential for decision-making to include local residents’ perceptions and preferences for ecosystem services (ES) and biodiversity (BD). This study conducted a questionnaire survey with 386 local residents to explore social perceptions for ES and BD in the Fenghe River watershed. ES contain food from agriculture (AGR), food from livestock (LIV), fresh water (FW), air purification (AP), water purification (WP), water retention (WR), soil retention (SR), aesthetics (AES), recreation (RE), and spirit (SP) in this study. Ranking and Likert scales are combined to identify residents’ preferences for ES and BD. The hypothetical market method is used to identify the willingness to pay for BD and ES. Independent sample T-test, one-way ANOVA, and Spearman correlation are used to analyze preference heterogeneity. The results show that: (1) residents prefer WP, AP, AGR, and FW; (2) 51.3% of respondents are willing to pay a fee for improving ES while 48.7% of respondents are unwilling to pay; (3) residents’ personal and residential factors affect preference heterogeneity. Last, we put forward three management suggestions on controlling environmental pollution, improving residents’ awareness of ES, and establishing a multi-channel fund based on government financial resources for improving ES in the Fenghe River watershed. Integrating stakeholders’ perceptions for ES into decision-making can promote the sustainable development in Fenghe River watershed.
Illuminating Stakeholder Perspectives at the Intersection of Air Quality Health Risk Communication and Cardiac Rehabilitation
Authors: Hano Mary Clare , Baghdikian Christina L. , Prince Steven , Lazzarino Elisa , Hubbell Bryan , Sams Elizabeth , Stone Susan , Davis Alison , Cascio Wayne E.
Published: September 2019

There is ample evidence of adverse cardiovascular health outcomes associated with exposure to air pollution and cardiac rehabilitation patients are at increased risk for future adverse health events related to air quality. Risk communication and health messaging about recommended behaviors to reduce exposure to air pollution can be integrated into existing care routines and structures. How this can be achieved most appropriately and effectively is not well understood. A focus group design is used to investigate cardiovascular patient and provider experiences, attitudes and beliefs about the risks of air pollution, related health risk messaging and factors that may influence integrating that topic into patient care and communication. Three discussions were hosted, one with cardiac patients, a second with non-physician cardiac rehabilitation providers and a third with physicians who treat cardiac patients. A within-case thematic inductive analysis of each discussion is used to understand the nature of communication, logistics, guidance and overall substance of the cardiac rehabilitation educational experience. Results suggest that air pollution may be an unrecognized risk factor for cardiac patients and cardiac rehabilitation is a prime setting for communicating air pollution health risk messaging. However, to effectively integrate air quality health risk messaging into cardiac rehabilitation, it is critical to account for the existing knowledge-base and behaviors of both providers and patients.
Optimization of Impervious Surface Space Layout for Prevention of Urban Rainstorm Waterlogging: A Case Study of Guangzhou, China
Authors: Yu Huafei , Zhao Yaolong , Fu Yingchun
Published: September 2019

With the rapid expansion of impervious surfaces, urban waterlogging has become a typical “urban disease” in China, seriously hindering the sustainable development of cities. Therefore, reducing the impact of impervious surfaces on surface runoff is an effective approach to alleviate urban waterlogging. Presently, the development mode of many cities in China has shifted from an increase in urban scale to the improvement of urban quality through urban renewal, which is the current and future development path for most cities. Optimizing the design of impervious surfaces in urban renewal planning to reduce its impact on surface runoff is an important way to prevent and control urban waterlogging. The aim of this research is to construct an optimization model of impervious surface space layout under the framework of a geographic simulation technology-integrated ant colony optimization (ACO) and Soil Conservation Service curve number (SCS-CN) model (ACO-SCS) in a case study of Guangzhou in China. Urban runoff plots in the study area are divided according to the area of the urban planning unit. With the goal of minimizing the runoff coefficient, the optimal space layout of the impervious surfaces is obtained, which provides a technical method and reference for urban waterlogging prevention and control through urban renewal planning. The results reveal that the optimization of impervious surface space layout through ACO-SCS achieves a satisfactory effect with an average optimization rate of 9.52%, and a maximum optimization rate of 33.16%. The research also shows that the initial impervious surface layout is the key influencing factor in ACO-SCS. In the urban renewal planning stage, the space layout of the impervious surfaces with a high–low–high density discontinuous connection can be constructed by transforming medium-density impervious surfaces into low-density impervious surfaces to achieve the flat and long-type agglomeration of the low-density and high-density impervious surfaces, which can effectively reduce the influence of urban development on surface runoff. There is spatial heterogeneity of the optimal results in different urban runoff plots. Therefore, the policy of urban renewal planning for urban waterlogging prevention and control should be different. The optimized results of impervious surface space layout provide useful reference information for urban renewal planning.
Analysis of a Historical Accident in a Spanish Coal Mine
Authors: Sanmiquel-Pera Lluís , Bascompta Marc , Anticoi Hernán Francisco
Published: September 2019

There has been a long history of coal mine accidents and these, usually, involve serious injuries, fatalities, and the destruction of facilities. In the seventies, an explosion killed 28 miners in a Spanish coal mine. This paper gives insight into the main factors of the accident by means of the causation mode, using two well-known alternatives: (1) the method from the Spanish Instituto Nacional de Seguridad y Salud en el Trabajo (INSST), where the causes and circumstances of the accident are classified into immediate causes and basic causes, and (2) the Feyer and Williamson method, where the classification is done using precursor events and contributing factors. The analysis identifies the lessons to be learned from the disaster. Both methods have given very similar results, verifying the goodness of the analysis. Methane emissions due to a variation in the exploitation method, the electrical installation, and a lack of safety procedures and training were the main causes of the accident. These findings explain the real causes of this accident and can be very valuable for the prevention of future accidents.
Prevalence of Chronic Diseases and Activity-Limiting Disability among Roma and Non-Roma People: A Cross-Sectional, Census-Based Investigation
Authors: Vincze Ferenc , Földvári Anett , Pálinkás Anita , Sipos Valéria , Janka Eszter Anna , Ádány Róza , Sándor János
Published: September 2019

The lack of recommended design for Roma health-monitoring hinders the interventions to improve the health status of this ethnic minority. We aim to describe the riskiness of Roma ethnicity using census-derived data and to demonstrate the value of census for monitoring the Roma to non-Roma gap. This study investigated the self-declared occurrence of at least one chronic disease and the existence of activity limitations among subjects with chronic disease by the database of the 2011 Hungarian Census. Risks were assessed by odds ratios (OR) and 95% confidence intervals (95% CI) from logistic regression analyses controlled for sociodemographic factors. Roma ethnicity is a risk factor for chronic diseases (OR = 1.17; 95% CI: 1.16–1.18) and for activity limitation in everyday life activities (OR = 1.20; 95% CI: 1.17–1.23), learning-working (OR = 1.24; 95% CI: 1.21–1.27), family life (OR = 1.22; 95% CI: 1.16–1.28), and transport (OR = 1.03; 95% CI: 1.01–1.06). The population-level impact of Roma ethnicity was 0.39% (95% CI: 0.37–0.41) for chronic diseases and varied between 0 and 1.19% for activity limitations. Our investigations demonstrated that (1) the Roma ethnicity is a distinct risk factor with significant population level impact for chronic disease occurrence accompanied with prognosis worsening influence, and that (2) the census can improve the Roma health-monitoring system, primarily by assessing the population level impact.
Service Level Factors Associated with Cervical Screening in Aboriginal and Torres Strait Islander Primary Health Care Centres in Australia
Authors: Diaz Abbey , Vo Brenda , Baade Peter D. , Matthews Veronica , Nattabi Barbara , Bailie Jodie , Whop Lisa J. , Bailie Ross , Garvey Gail
Published: September 2019

Aboriginal and Torres Strait Islander women have significantly higher cervical cancer incidence and mortality than other Australian women. In this study, we assessed the documented delivery of cervical screening for women attending Indigenous Primary Health Care (PHC) centres across Australia and identified service-level factors associated with between-centre variation in screening coverage. We analysed 3801 clinical audit records for PHC clients aged 20–64 years from 135 Indigenous PHC centres participating in the Audit for Best Practice in Chronic Disease (ABCD) continuous quality improvement (CQI) program across five Australian states/territories during 2005 to 2014. Multilevel logistic regression models were used to identify service-level factors associated with screening, while accounting for differences in client-level factors. There was substantial variation in the proportion of clients who had a documented cervical screen in the previous two years across the participating PHC centres (median 50%, interquartile range (IQR): 29–67%), persisting over years and audit cycle. Centre-level factors explained 40% of the variation; client-level factors did not reduce the between-centre variation. Screening coverage was associated with longer time enrolled in the CQI program and very remote location. Indigenous PHC centres play an important role in providing cervical screening to Aboriginal and Torres Strait Islander women. Thus, their leadership is essential to ensure that Australia’s public health commitment to the elimination of cervical cancer includes Aboriginal and Torres Strait Islander women. A sustained commitment to CQI may improve PHC centres delivery of cervical screening; however, factors that may impact on service delivery, such as organisational, geographical and environmental factors, warrant further investigation.
Sun Exposure of Body Districts: Development and Validation of an Algorithm to Predict the Erythemal Ultra Violet Dose
Authors: Salvadori Giacomo , Lista Davide , Burattini Chiara , Gugliermetti Luca , Leccese Francesco , Bisegna Fabio
Published: September 2019

Solar Ultra-Violet (UV) radiation has positive and negative effects on human body tissues. Small doses of solar UV radiation are needed by the human skin for the activation of the vitamin D production. Overexposure to solar UV radiation can produce acute and long-term negative effects, such as sunburns and, in the worst cases, cataracts and skin cancers. For this reason, knowing the amount of UV doses received by people is essential to evaluate their risk to UV overexposure and to evaluate the adequate countermeasure to avoid the negative effects. The original contribution of the present study consists in having searched, collected, adapted and processed a series of technical information and analytical relations, developing an algorithm suitable for the calculation of the erythemal UV dose on sloped surfaces exposed to solar radiation, which at the moment is not present in the scientific literature. The results obtained by the algorithm have been compared to the results of a field measurements campaign, carried out in three different Italian sites. Results comparison indicated that measured and calculated values show a sufficient level of agreement, with a mean absolute error equal to 20%.
Structure and Dynamics of Mono- vs. Doubly Lipidated Rab5 in Membranes
Authors: Münzberg Eileen , Stein Matthias
Published: September 2019

The Rab5 small GTPase is a regulator of endosomal trafficking and vesicle fusion. It possesses two adjacent cysteine residues for post-translational geranylgeranylation at its C-terminus for the protein to associate with the early endosome membrane. We compare the effect of mono-lipidification of only one cysteine residue with the doubly modified, fully functional Rab protein in both guanosine diphosphate (GDP)- and guanosine triphosphate (GTP)-bound states and in different membranes (one, three, and six-component membranes). Molecular simulations show that the mono-geranylgeranylated protein is less strongly associated with the membranes and diffuses faster than the doubly lipidated protein. The geranylgeranyl anchor membrane insertion depth is smaller and the protein–membrane distance distribution is broad and uncharacteristic for the membrane composition. The mono-geranylgeranylated protein reveals an unspecific association with the membrane and an orientation at the membrane that does not allow a nucleotide-specific recruitment of further effector proteins. This work shows that double-lipidification is critical for Rab5 to perform its physiological function and mono-geranylgeranylation renders it membrane-associated but non-functional.
Low Energy Shock Wave Therapy Inhibits Inflammatory Molecules and Suppresses Prostatic Pain and Hypersensitivity in a Capsaicin Induced Prostatitis Model in Rats
Authors: Wang Hung-Jen , Tyagi Pradeep , Chen Yu-Ming , Chancellor Michael B. , Chuang Yao-Chi
Published: September 2019

The effect of low energy shock wave (LESW) therapy on the changes of inflammatory molecules and pain reaction was studied in a capsaicin (10 mM, 0.1 cc) induced prostatitis model in rats. Intraprostatic capsaicin injection induced a pain reaction, including closing of the eyes, hypolocomotion, and tactile allodynia, which effects were ameliorated by LESW treatment. LESW therapy (2Hz, energy flux density of 0.12 mJ/mm2) at 200 and 300 shocks significantly decreased capsaicin-induced inflammatory reactions, reflected by a reduction of tissue edema and inflammatory cells, COX-2 and TNF-α stained positive cells, however, the therapeutic effects were not observed at 100 shocks treated group. Capsaicin-induced IL-1β, COX-2, IL-6, caspase-1, and NGF upregulation on day 3 and 7, while NALP1 and TNF-α upregulation was observed on day 7. LESW significantly suppressed the expression of IL-1β, COX-2, caspase-1, NGF on day 3 and IL-1β, TNF-α, COX-2, NALP1, caspase-1, NGF expression on day 7 in a dose-dependent fashion. LESW has no significant effect on IL-6 expression. Intraprostatic capsaicin injection activates inflammatory molecules and induces prostatic pain and hypersensitivity, which effects were suppressed by LESW. These findings might be the potential mechanisms of LESW therapy for nonbacterial prostatitis in humans.
Optimal Isolation Method of Small Extracellular Vesicles from Rat Plasma
Authors: Otani Kosuke , Fujioka Yusei , Okada Muneyoshi , Yamawaki Hideyuki
Published: September 2019

Small extracellular vesicles (sEVs) mediate cell–to–cell communication. We recently reported that circulating sEVs regulate systolic blood pressure in an animal model of human systemic hypertension. However, the underlying mechanisms still remain to be elucidated. As the first step for detailed analyses, we sought to increase the yield and purity of sEVs isolated from rat plasma. We compared the concentration and size distribution of sEVs as well as protein expression of the sEV marker and contaminants among plasma sEVs isolated by the ultracentrifugation (UC) method, the precipitation with polyethylene-glycol and ultracentrifugation (PEG-UC) method, or the precipitation with polyethylene-glycol (PEG) method. Effects of anticoagulants were also examined. The total concentration of plasma sEVs isolated by the PEG or PEG-UC method was much higher than that of the UC method. In the plasma sEVs isolated by the PEG-UC method, contaminating proteins were lower, while the protein expression of certain sEV markers was higher than that of the PEG method. There was no significant difference in total concentration or protein expression of sEV markers in sEVs isolated from rat plasma treated with three different anticoagulants (heparin, ethylenediaminetetraacetic acid, or acid citrate dextrose buffer) by the PEG-UC method. We, for the first time, determined that the PEG-UC method was optimal for sEV isolation from rat plasma.
Strong Signs for a Weak Wall in Tricuspid Aortic Valve Associated Aneurysms and a Role for Osteopontin in Bicuspid Aortic Valve Associated Aneurysms
Authors: Stern Christian , Scharinger Bernhard , Tuerkcan Adrian , Nebert Clemens , Mimler Teresa , Baranyi Ulrike , Doppler Christian , Aschacher Thomas , Andreas Martin , Stelzmueller Marie-Elisabeth , Ehrlich Marek , Graf Alexandra , Laufer Guenther , Bernhard David , Messner Barbara
Published: September 2019

Central processes in the pathogenesis of TAV- (tricuspid aortic valve) and BAV- (bicuspid aortic valve) associated ascending thoracic aortic aneurysm (ATAA) development are still unknown. To gain new insights, we have collected aortic tissue and isolated smooth muscle cells of aneurysmal tissue and subjected them to in situ and in vitro analyses. We analyzed aortic tissue from 78 patients (31 controls, 28 TAV-ATAAs, and 19 BAV-ATAAs) and established 30 primary smooth muscle cell cultures. Analyses included histochemistry, immuno-, auto-fluorescence-based image analyses, and cellular analyses including smooth muscle cell contraction studies. With regard to TAV associated aneurysms, we observed a strong impairment of the vascular wall, which appears on different levels—structure and dimension of the layers (reduced media thickness, increased intima thickness, atherosclerotic changes, degeneration of aortic media, decrease of collagen, and increase of elastic fiber free area) as well as on the cellular level (accumulation of fibroblasts/myofibroblasts, and increase in the number of smooth muscle cells with a reduced alpha smooth muscle actin (α-SM actin) content per cell). The pathological changes in the aortic wall of BAV patients were much less pronounced—apart from an increased expression of osteopontin (OPN) in the vascular wall which stem from smooth muscle cells, we observed a trend towards increased calcification of the aortic wall (increase significantly associated with age). These observations provide strong evidence for different pathological processes and different disease mechanisms to occur in BAV- and TAV-associated aneurysms.
New Insights into Immunotherapy Strategies for Treating Autoimmune Diabetes
Authors: Cabello-Olmo Miriam , Araña Miriam , Radichev Ilian , Smith Paul , Huarte Eduardo , Barajas Miguel
Published: September 2019

Type 1 diabetes mellitus (T1D) is an autoimmune illness that affects millions of patients worldwide. The main characteristic of this disease is the destruction of pancreatic insulin-producing beta cells that occurs due to the aberrant activation of different immune effector cells. Currently, T1D is treated by lifelong administration of novel versions of insulin that have been developed recently; however, new approaches that could address the underlying mechanisms responsible for beta cell destruction have been extensively investigated. The strategies based on immunotherapies have recently been incorporated into a panel of existing treatments for T1D, in order to block T-cell responses against beta cell antigens that are very common during the onset and development of T1D. However, a complete preservation of beta cell mass as well as insulin independency is still elusive. As a result, there is no existing T1D targeted immunotherapy able to replace standard insulin administration. Presently, a number of novel therapy strategies are pursuing the goals of beta cell protection and normoglycemia. In the present review we explore the current state of immunotherapy in T1D by highlighting the most important studies in this field, and envision novel strategies that could be used to treat T1D in the future.
Association with PD-L1 Expression and Clinicopathological Features in 1000 Lung Cancers: A Large Single-Institution Study of Surgically Resected Lung Cancers with a High Prevalence of EGFR Mutation
Authors: Lee Seung Eun , Kim Yu Jin , Sung Minjung , Lee Mi-Sook , Han Joungho , Kim Hong Kwan , Choi Yoon-La
Published: September 2019

Programmed cell death ligand 1 (PD-L1) expression is an important biomarker for predicting response to immunotherapy in clinical practice. Hence, identification and characterization of factors that predict high expression of PD-L1 in patients is critical. Various studies have reported the association of PD-L1 expression with driver genetic status in non-small cell cancer; however, the results have been conflicting and inconclusive. We analyzed the relationship between PD-L1 expression and clinicopathological factors including driver genetic alterations in 1000 resected lung cancers using a clinically validated PD-L1 immunohistochemical assay. PD-L1 expression was significantly higher in squamous cell carcinoma (SCC) compared to adenocarcinomas. PD-L1 expression in adenocarcinoma was associated with higher N-stage, solid histologic pattern, EGFR wild type, and ALK positive, but no significant association with the clinicopathological factors in SCC. EGFR mutant adenocarcinomas with distinctive clinicopathologic features, especially solid histologic pattern and higher stage showed higher PD-L1 expression. To the best of our knowledge, this study is the largest to evaluate the association between PD-L1 expression and clinicopathological and molecular features in lung cancer with a highly prevalent EGFR mutation. Therefore, our results are useful to guide the selection of lung cancer, even EGFR-mutated adenocarcinoma patients with PD-L1 expression, for further immunotherapy.
Cytosolic Phospholipase A2 Alpha Regulates TLR Signaling and Migration in Metastatic 4T1 Cells
Authors: Tunset Hanna Maja , Feuerherm Astrid Jullumstrø , Selvik Linn-Karina Myrland , Johansen Berit , Moestue Siver Andreas
Published: September 2019

Metastatic disease is the leading cause of death in breast cancer patients. Disrupting the cancer cell’s ability to migrate may be a strategy for hindering metastasis. Cytosolic phospholipase A2 α (cPLA2α), along with downstream proinflammatory and promigratory metabolites, has been implicated in several aspects of tumorigenesis, as well as metastasis, in various types of cancer. In this study, we aim to characterize the response to reduced cPLA2α activity in metastatic versus non-metastatic cells. We employ an isogenic murine cell line pair displaying metastatic (4T1) and non-metastatic (67NR) phenotype to investigate the role of cPLA2α on migration. Furthermore, we elucidate the effect of reduced cPLA2α activity on global gene expression in the metastatic cell line. Enzyme inhibition is achieved by using a competitive pharmacological inhibitor, cPLA2α inhibitor X (CIX). Our data show that 4T1 expresses significantly higher cPLA2α levels as compared to 67NR, and the two cell lines show different sensitivity to the CIX treatment with regards to metabolism and proliferation. Inhibition of cPLA2α at nontoxic concentrations attenuates migration of highly metastatic 4T1 cells, but not non-metastatic 67NR cells. Gene expression analysis indicates that processes such as interferon type I (IFN-I) signaling and cell cycle regulation are key processes regulated by cPLA2a in metastatic 4T1 cells, supporting the findings from the biological assays. This study demonstrates that two isogenic cancer cell lines with different metastatic potential respond differently to reduced cPLA2α activity. In conclusion, we argue that cPLA2α is a potential therapeutic target in cancer and that enzyme inhibition may inhibit metastasis through an anti-migratory mechanism, possibly involving Toll-like receptor signaling and type I interferons.
Poria cocus Wolf Extract Ameliorates Hepatic Steatosis through Regulation of Lipid Metabolism, Inhibition of ER Stress, and Activation of Autophagy via AMPK Activation
Authors: Kim Ji-Hyun , Sim Hyun A , Jung Dae Young , Lim Eun Yeong , Kim Yun Tai , Kim Byung Joo , Jung Myeong Ho
Published: September 2019

Poria cocos Wolf (PCW) is an edible, pharmaceutical mushroom with remarkable biological properties including anti-tumor, anti-inflammation, anti-oxidation, anti-ageing, and anti-diabetic effects. In the current study, we investigated the effects of PCW extract on hepatic steatosis under in vitro and in vivo conditions, and elucidated the underlying mechanisms. In this study, a mixture of HepG2 cells treated with free fatty acid (FFA)—palmitic and oleic acid—and high-fat diet (HFD)-fed obese mice were used; in this background, the triglyceride (TG) levels in HepG2 cells and mice liver were measured, and the expression levels of genes associated with lipogenesis, fatty acid oxidation, endoplasmic reticulum (ER) stress, and autophagy were determined. Treatment of HepG2 cells with FFA enhanced intracellular TG levels in HepG2 cells, but co-treatment with PCW significantly attenuated the TG levels. Notably, PCW significantly enhanced the phosphorylation of AMP-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), and sterol regulatory element-binding protein-1c (SREBP-1c) in FFA-treated HepG2 cells. PCW downregulated the expression of lipogenesis-related genes, but upregulated the expression of genes associated with fatty acid oxidation. Further, PCW inhibited FFA-induced expression of ER stress markers and induced autophagy proteins. However, inhibition of AMPK significantly attenuated the beneficial effects of PCW in HepG2 cells. Moreover, PCW efficiently decreased HFD-induced hepatic TG accumulation in vivo and increased the phosphorylation of hepatic AMPK. Three compounds present in PCW including poricoic acid, pachymic acid, and ergosterol, significantly decreased FFA-induced increase in intracellular TG levels, consistent with increased AMPK phosphorylation, suggesting that poricoic acid, pachymic acid, and ergosterol are responsible for PCW-mediated amelioration of hepatic steatosis. Taken together, these results demonstrated that PCW ameliorates hepatic steatosis through the regulation of lipid metabolism, inhibition of ER stress, and activation of autophagy in an AMPK-dependent manner. This suggested that PCW can be potentially used for the treatment of hepatic steatosis.
Tumour-Derived Extracellular Vesicles (EVs): A Dangerous “Message in A Bottle” for Bone
Authors: Cappariello Alfredo , Rucci Nadia
Published: September 2019

Several studies have shown the importance of Extracellular Vesicles (EVs) in the intercellular communication between tumour and resident cells. Through EVs, tumour cells can trigger cell-signalling molecules and shuttle exogenous information to target cells, thus promoting spread of the disease. In fact, many processes are fuelled by EVs, such as tumour invasion and dormancy, drug-resistance, immune-surveillance escape, extravasation, extracellular matrix remodelling and metastasis. A key element is certainly the molecular profile of the shed cargo. Understanding the biochemical basis of EVs would help to predict the ability and propensity of cancer cells to metastasize a specific tissue, with the aim to target the release of EVs and to manipulate their content as a possible therapeutic approach. Moreover, EV profiling could help monitor the progression of cancer, providing a useful tool for more effective therapy. This review will focus on all the EV-mediated mentioned mechanisms in the context of both primary bone cancers and bone metastases.
Expression and Distribution of the Auxin Response Factors in Sorghum bicolor During Development and Temperature Stress
Authors: Chen Dan , Wang Weian , Wu Yaqin , Xie Hui , Zhao Linfei , Zeng Qi , Zhan Yonghua
Published: September 2019

Auxin response factor (ARF) is a transcription factor that can specifically bind to the promoter of auxin-responsive genes in plants and plays an important regulatory role in plant growth and development. The previous studies have predicted 25 ARF genes in Sorghum bicolor (SbARFs) and indicated that SbARFs play complex roles in salt and drought stresses. In this study, we reclassified and analyzed the structures of ARFs in three plants, including sorghum, rice, and Arabidopsis. Phylogenetic analyses categorized 73 ARF into five classes. By studying the characterization of the structures, it was found that SbARFs from the same evolutionary branches showed similar motif patterns. Furthermore, the expression patterns of SbARF genes during development and temperature stress were investigated in sorghum. Quantitative transcription-quantitative polymerase chain reaction (qRT-PCR) results suggested that they had different expression patterns in vegetative and reproductive organs at various developmental stages. High and low-temperature treatments and qRT-PCR demonstrated some of them changed dramatically along with the increase of treatment time. Additionally, in situ hybridization results displayed that SbARF genes were accumulated in vascular tissues under temperature stress. These findings provide evidence that SbARFs may play important roles in sorghum vegetative development, reproductive development, and auxin response to temperature stress.
Identification of New DR5 Agonistic Nanobodies and Generation of Multivalent Nanobody Constructs for Cancer Treatment
Authors: Sadeghnezhad Golnaz , Romão Ema , Bernedo-Navarro Robert , Massa Sam , Khajeh Khosro , Muyldermans Serge , Hassania Sadegh
Published: September 2019

Current cancer therapeutics suffer from a lack of specificity in targeting tumor cells and cause severe side effects. Therefore, the design of highly specialized drugs comprising antibody derivatives inducing apoptosis in targeted cancer cells is considered to be a promising strategy. Drugs acting on death receptor 5 (DR5) such as DR5 agonist antibodies replacing “TNF-related apoptosis-inducing ligand” (TRAIL) offer feasible opportunities in this direction. Although such agonists provided good antitumor activity in preclinical studies, they were less effective in clinical studies, possibly due to a disturbed Fc interaction with Fc-γ receptors. Thus, multimerized antigen binding fragments without Fc have been proposed to increase their efficacy. We generated nanobodies (Nbs), recombinant variable domains of heavy chain-only antibodies of camelids, against the DR5 ectodomain. Nb24 and Nb28 had an affinity in the nM and sub-nM range, but only Nb28 competes with TRAIL for binding to DR5. Bivalent, trivalent, and tetravalent constructs were generated, as well as an innovative pentameric Nb complex, to provoke avidity effects. In our cellular assays, these trimeric, tetrameric, and pentameric Nbs have a higher apoptotic capacity than monomeric Nbs and seem to mimic the activity of the natural TRAIL ligand on various cancer cells.
Subunits Inference and Lexicon Development Based on Pairwise Comparison of Utterances and Signs
Authors: Tornay Sandrine , Magimai.-Doss Mathew
Published: September 2019

Communication languages convey information through the use of a set of symbols or units. Typically, this unit is word. When developing language technologies, as words in a language do not have the same prior probability, there may not be sufficient training data for each word to model. Furthermore, the training data may not cover all possible words in the language. Due to these data sparsity and word unit coverage issues, language technologies employ modeling of subword units or subunits, which are based on prior linguistic knowledge. For instance, development of speech technologies such as automatic speech recognition system presume that there exists a phonetic dictionary or at least a writing system for the target language. Such knowledge is not available for all languages in the world. In that direction, this article develops a hidden Markov model-based abstract methodology to extract subword units given only pairwise comparison between utterances (or realizations of words in the mode of communication), i.e., whether two utterances correspond to the same word or not. We validate the proposed methodology through investigations on spoken language and sign language. In the case of spoken language, we demonstrate that the proposed methodology can lead up to discovery of phone set and development of phonetic dictionary. In the case of sign language, we demonstrate how hand movement information can be effectively modeled for sign language processing and synthesized back to gain insight about the derived subunits.
Hydrodynamic Effects Produced by Submerged Breakwaters in a Coastal Area with a Curvilinear Shoreline
Authors: Gallerano Francesco , Cannata Giovanni , Palleschi Federica
Published: September 2019

A three-dimensional numerical study of the hydrodynamic effect produced by a system of submerged breakwaters in a coastal area with a curvilinear shoreline is proposed. The three-dimensional model is based on an integral contravariant formulation of the Navier-Stokes equations in a time-dependent curvilinear coordinate system. The integral form of the contravariant Navier-Stokes equations is numerically integrated by a finite-volume shock-capturing scheme which uses Monotonic Upwind Scheme for Conservation Laws Total Variation Diminishing (MUSCL-TVD) reconstructions and an Harten Lax van Leer Riemann solver (HLL Riemann solver). The numerical model is used to verify whether the presence of a submerged coastal defence structure, in the coastal area with a curvilinear shoreline, is able to modify the wave induced circulation pattern and the hydrodynamic conditions from erosive to accretive.
Temporal Model for Ship Twin-Propeller Jet Induced Sandbed Scour
Authors: Cui Yonggang , Lam Wei Haur , Zhang Tianming , Sun Chong , Robinson Desmond , Hamill Gerard
Published: September 2019

This research paper proposes the use of empirical equations to estimate the temporal maximum scour that is induced by twin-propeller ( ε t w i n = Ω t [ l n ( t ) ] Γ t ) when acting over non-cohesive bed materials. A purpose built experimental apparatus is used to obtain the measurement data required for the calculation of the empirical constants. The output from rigorous experimental investigations demonstrates that the maximum scour depth produced from the operation of twin-propeller ( ε t w i n ), within the confines of a harbour basin, varies as a logarithmic function of time. A dimensional analysis of the standard single propeller configuration is used as the foundation upon which the scour equation is postulated. This is extended to include the influence of the operating distance between the twin-propeller configurations for the first time. The division of scours by twin-propeller and single-propeller ( ε twin / ε m ) enables the establishment of mathematical relation to calculate C1, C2, A, and B. The constants are C 1 = 366.11, C 2 = 0.3376, A = 0.859, and B = 0.1571. The proposed scour equation is more reliable within the time zone up to two hours based on the experimental data.
Correcting the Bias in the Practitioner Black-Scholes Method
Authors: Yin Yun , Moffatt Peter G.
Published: September 2019

We address a number of technical problems with the popular Practitioner Black-Scholes (PBS) method for valuing options. The method amounts to a two-stage procedure in which fitted values of implied volatilities (IV) from a linear regression are plugged into the Black-Scholes formula to obtain predicted option prices. Firstly we ensure that the prediction from stage one is positive by using log-linear regression. Secondly, we correct the bias that results from the transformation applied to the fitted values (i.e., the Black-Scholes formula) being a highly non-linear function of implied volatility. We apply the smearing technique in order to correct this bias. An alternative means of implementing the PBS approach is to use the market option price as the dependent variable and estimate the parameters of the IV equation by the method of non-linear least squares (NLLS). A problem we identify with this method is one of model incoherency: the IV equation that is estimated does not correspond to the set of option prices used to estimate it. We use the Monte Carlo method to verify that (1) standard PBS gives biased option values, both in-sample and out-of-sample; (2) using standard (log-linear) PBS with smearing almost completely eliminates the bias; (3) NLLS gives biased option values, but the bias is less severe than with standard PBS. We are led to conclude that, of the range of possible approaches to implementing PBS, log-linear PBS with smearing is preferred on the basis that it is the only approach that results in valuations with negligible bias.
Micromechanical Modeling of Damage Evolution and Mechanical Behaviors of CF/Al Composites under Transverse and Longitudinal Tensile Loadings
Authors: Wang Zhenjun , Yang Siyuan , Du Zehui , Jiang Wugui , Zhang Aodi , Cai Changchun , Yang Wei
Published: September 2019

This paper investigates the progressive damage and failure behavior of unidirectional graphite fiber-reinforced aluminum composites (CF/Al composites) under transverse and longitudinal tensile loadings. Micromechanical finite element analyses are carried out using different assumptions regarding fiber, matrix alloy, and interface properties. The validity of these numerical analyses is examined by comparing the predicted stress-strain curves with the experimental data measured under transverse and longitudinal tensile loadings. Assuming a perfect interface, the transverse tensile strength is overestimated by more than 180% and the transverse fracture induced by fiber failure is unrealistic based on the experimental observations. In fact, the simulation and experiment results indicate that the interface debonding arising from the matrix alloy failure dominates the transverse fracture, and the influence of matrix alloy properties on the mechanical behavior is inconspicuous. In the case of longitudinal tensile testing, however, the characteristic of interface bonding has no significant effect on the macroscopic mechanical response due to the low in-situ strength of the fibers. It is demonstrated that ultimate longitudinal fracture is mainly controlled by fiber failure mechanisms, which is confirmed by the fracture morphology of the tensile samples.
Influence of Different Pretreatments on the Antibacterial Properties of Chitosan Functionalized Viscose Fabric: TEMPO Oxidation and Coating with TEMPO Oxidized Cellulose Nanofibrils
Authors: Korica Matea , Peršin Zdenka , Trifunović Snežana , Mihajlovski Katarina , Nikolić Tanja , Maletić Slavica , Fras Zemljič Lidija , Kostić Mirjana M.
Published: September 2019

The main objective of this study was to obtain chitosan functionalized viscose fabric with improved antibacterial properties and washing durability. In this regard carboxyl and aldehyde groups, as binding points for irreversible chitosan attachment into/onto viscose fabric, were introduced by two different pretreatments: 2,2,6,6-tetramethylpiperidine-1-oxy radical (TEMPO) oxidation and coating with TEMPO oxidized cellulose nanofibrils (TOCN). The Fourier transform infrared spectroscopy, elemental analysis, zeta potential measurements, scanning electron microscopy, breaking strength and antibacterial testing were used to evaluate the influence of these pretreatments on chitosan binding, but also on chemical, electrokinetic, morphological, mechanical and antibacterial properties of pretreated and chitosan functionalized viscose fabrics. Washing durability of chitosan functionalized viscose was monitored through changes in the chitosan content, electrokinetic and antibacterial properties after multiple washing. TOCN coating improves mechanical properties of fabric, while TEMPO oxidation deteriorates them. The results show that both pretreatments improve chitosan adsorption and thus antibacterial properties, which are highly durable to washing. After five washings, the chitosan functionalized pretreated viscose fabrics preserve their antibacterial activity against Staphylococcus aureus, while antibacterial activity against Escherichia coli was lost. TOCN coated and chitosan functionalized viscose fabric is a high value-added product with simultaneously improved antibacterial and mechanical properties, which may find application as medical textiles.
Optical Dispersions of Bloch Surface Waves and Surface Plasmon Polaritons: Towards Advanced Biosensors
Authors: Balevicius Zigmas , Baskys Algirdas
Published: September 2019

The total internal reflection ellipsometry (TIRE) method was used for the excitation and study of the sensitivity features of surface plasmon polariton (SPP) and Bloch surface waves (BSWs) resonances. For the BSWs generation distributed Bragg gratings were formed on the tops of the substrates (BK7 glass substrate), which had six bilayers of ~120 nm SiO2 and ~40 nm TiO2 and 40 nm of TiO2 on the top. The SPP sample consisted of the BK7 glass prism and a gold layer (45 nm). Numerical calculations of the optical dispersions and the experimental TIRE data have shown that SPP resonance overtake the BSWs in wavelength scanning by a factor of about 17. However, for the ellipsometric parameters Ψ and Δ in the vicinity of excitations, the BSW sensitivity is comparable with SPP. The obtained resolutions were Δ S P P = 7.14 × 10 − 6 R I U , Ψ S P P = 1.7 × 10 − 5 R I U for the SPP and Δ B S W = 8.7 × 10 − 6 R I U , Ψ B S W = 2.7 × 10 − 5 R I U for the BSW. The capabilities of both surface excitations are discussed from the sensitivity point of view in the design of these advanced biosensors.