bluuebunny/crossref_metadata_2025 huggingface dataset:
Characteristics of drought propagation in South Korea: relationship between meteorological, agricultural, and hydrological droughts
Authors: Bae Hyedeuk , Ji Heesook , Lim Yoon-Jin , Ryu Young , Kim Moon-Hyun , Kim Baek-Jo
Published: September 2019

To investigate the propagation of meteorological droughts to agricultural and hydrological droughts, the relationship between droughts was analyzed using observed precipitation and agricultural reservoir and dam storage levels with SPI from 1998 to 2015 in South Korea. For the relationship between different types of droughts, we find that the occurrence of meteorological droughts after concentrated precipitation in the wet season (from June to September) tends not to lead to agricultural or hydrological droughts. A lack of precipitation from April to September, when large volumes of irrigation water are consumed, triggers both meteorological and agricultural droughts. In the case of hydrological droughts propagated from meteorological droughts, precipitation deficits in the dry season (between October and March) caused decrease in dam storage levels only. The occurrence of all different types of droughts is associated with extreme meteorological droughts, which are mainly caused by precipitation deficits in the wet season or prolonged rainfall shortages; in these cases, meteorological droughts led to agricultural and hydrological droughts. An analysis of the seasonal characteristics of storage level changes that in the wet season, agricultural reservoir storage levels are more dependent on precipitation deficits than dam storage levels. On other hand, when precipitation deficits were recorded in the dry season, agricultural reservoir storage levels went up, but dam storage levels dropped. The propagation of meteorological droughts to agricultural and hydrological droughts depends not only on drought severity but also on the drought timing. These findings may contribute to establishing a comprehensive drought monitoring system.
In vivo photoprotection mechanisms observed from leaf spectral absorbance changes showing VIS–NIR slow-induced conformational pigment bed changes
Authors: Van Wittenberghe Shari , Alonso Luis , Malenovský Zbyněk , Moreno José
Published: September 2019

Regulated heat dissipation under excessive light comprises a complexity of mechanisms, whereby the supramolecular light-harvesting pigment–protein complex (LHC) shifts state from light harvesting towards heat dissipation, quenching the excess of photo-induced excitation energy in a non-photochemical way. Based on whole-leaf spectroscopy measuring upward and downward spectral radiance fluxes, we studied spectrally contiguous (hyperspectral) transient time series of absorbance A(λ,t) and passively induced chlorophyll fluorescence F(λ,t) dynamics of intact leaves in the visible and near-infrared wavelengths (VIS–NIR, 400–800 nm) after sudden strong natural-like illumination exposure. Besides light avoidance mechanism, we observed on absorbance signatures, calculated from simultaneous reflectance R(λ,t) and transmittance T(λ,t) measurements as A(λ,t) = 1 − R(λ,t) − T(λ,t), major dynamic events with specific onsets and kinetical behaviour. A consistent well-known fast carotenoid absorbance feature (500–570 nm) appears within the first seconds to minutes, seen from both the reflected (backscattered) and transmitted (forward scattered) radiance differences. Simultaneous fast Chl features are observed, either as an increased or decreased scattering behaviour during quick light adjustment consistent with re-organizations of the membrane. The carotenoid absorbance feature shows up simultaneously with a major F decrease and corresponds to the xanthophyll conversion, as quick response to the proton gradient build-up. After xanthophyll conversion (t = 3 min), a kinetically slower but major and smooth absorbance increase was occasionally observed from the transmitted radiance measurements as wide peaks in the green (~ 550 nm) and the near-infrared (~ 750 nm) wavelengths, involving no further F quenching. Surprisingly, in relation to the response to high light, this broad and consistent VIS–NIR feature indicates a slowly induced absorbance increase with a sigmoid kinetical behaviour. In analogy to sub-leaf-level observations, we suggest that this mechanism can be explained by a structure-induced low-energy-shifted energy redistribution involving both Car and Chl. These findings might pave the way towards a further non-invasive spectral investigation of antenna conformations and their relations with energy quenching at the intact leaf level, which is, in combination with F measurements, of a high importance for assessing plant photosynthesis in vivo and in addition from remote observations.
Governance under the shadow of the law: trading high value fine art
Authors: Shortland Anja , Shortland Andrew
Published: September 2019

The market for paintings by well-known artists is booming despite widespread concern about art crime and difficulties in establishing provenance. Public law enforcement is imperfect, and court cases often are deemed problematic. So how is the thriving art market governed in practice? We analyze the protocols used by the top auction houses to identify and resolve problems of illicit supply—fakes, forgeries and items with defective legal titles—through the lens of institutional analysis. We uncover a polycentric private governance system in which different actors govern distinct but overlapping issue areas, motivated by profit, prestige, or the search for truth. When the financial stakes rise, opportunistic behavior undermines the credibility of private governance. We argue that as litigious, super-rich investors entered the art market, the interaction between public law and the traditional private governance system restricted the supply of “blue chip” art, driving the escalation of prices.
High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana
Authors: Pavicic M. , Wang F. , Mouhu K. , Himanen K.
Published: September 2019

Seed quality is an important factor for seedling vigour as well as adult plant resilience. The key quality attributes are related to physical characteristics, physiological performance, genetic background and health status of the seeds. Many ways to address seed quality attributes have been developed and recently many of them have featured automated high throughput methods. In our study, we addressed two of the seed quality attributes, namely physiological performance and genetic background by analysing germination rates in our mutant collection. These mutants represent ubiquitin E3 ligases that transcriptionally respond to abscisic acid (ABA). This plant hormone is an important regulator of germination and seedling establishment. To facilitate in vitro germination screens of large seed collections a high throughput image-based assay was developed. As a read out of the germination on ABA treatment the cotyledon emergence was detected with top view chlorophyll fluorescence camera. By applying the ABA treatment during germination, RING-type ubiquitin E3 ligase mutants were identified, showing either resistant or sensitive responses to ABA. In conclusion, a scalable high throughput screen for in vitro germination assay was established that allowed fast screening of tens of mutants in a hormone supplemented media.
APASL HCV guidelines of virus-eradicated patients by DAA on how to monitor HCC occurrence and HBV reactivation
Authors: Kanda Tatsuo , Lau George K. K. , Wei Lai , Moriyama Mitsuhiko , Yu Ming-Lung , Chuang Wang-Long , Ibrahim Alaaeldin , Lesmana Cosmas Rinaldi Adithya , Sollano Jose , Kumar Manoj , Jindal Ankur , Sharma Barjesh Chander , Hamid Saeed S. , Kadir Dokmeci A. , Mamun-Al-Mahtab  , McCaughan Geoffrey W. , Wasim Jafri , Crawford Darrell H. G. , Kao Jia-Horng , Ooka Yoshihiko , Yokosuka Osamu , Sarin Shiv Kumar , Omata Masao
Published: September 2019

In the direct-acting antiviral (DAA) era for hepatitis C virus (HCV) infection, sustained virological response (SVR) is very high, but close attention must be paid to the possible occurrence of hepatocellular carcinoma (HCC) and reactivation of hepatitis B virus (HBV) in patients with co-infection who achieved SVR in short term. HCC occurrence was more often observed in patients with previous HCC history. We found occurrence of HCC in 178 (29.6%) of 602 patients with previous HCC history (15.4 months mean follow-up post-DAA initiation) but, in contrast, in only 604 (1.3%) of 45,870 patients without previous HCC history (18.2 months mean follow-up). Thus, in these guidelines, we recommend the following: in patients with previous HCC history, surveillance at 4-month intervals for HCC by ultrasonography (US) and tumor markers should be performed. In patients without previous HCC history, surveillance at 6- to 12-month intervals for HCC including US is recommended until the long-term DAA treatment effects, especially for the resolution of liver fibrosis, are confirmed. This guideline also includes recommendations on how to follow-up patients who have been infected with both HCV and HBV. When HCV was eradicated in these HBsAg-positive patients or patients with previous HBV infection (anti-HBc and/or anti-HBs-positive), it was shown that HBV reactivation or HBV DNA reappearance was observed in 67 (41.4%) of 162 or 12 (0.9%) of 1317, respectively. For these co-infected patients, careful attention should be paid to HBV reactivation for 24 weeks post-treatment.
Petrochemical Steel Pipe Rack: Critical Assessment of Existing Design Code Provisions and a Case Study
Authors: Di Sarno Luigi , Karagiannakis George
Published: September 2019

The investigation of the seismic integrity of petrochemical plant steel structures should be commensurable to their importance given the high necessity for human life safety and financial robustness. To date, it is demonstrated in the existing literature that still many grey areas of knowledge exist upon the appropriate application of code provisions on non-building structures design. Indeed, the selection of seismic design parameters such as system performance factors or important classes are still vague aspects, in contrast with those for common building structures, either because of the paucity of information of seismic codes or due to the structural peculiarities that characterise the industrial structures resulting in the difficulty of defining ‘all-encompassing’ design parameters. The present paper aims at highlighting those parameters considering also a case-study that pertains to a steel pipe rack. The pipe rack is designed and analysed in the linear and nonlinear regime, both statically and dynamically, according to the Italian and European codes. American code provisions are examined as well so as possible inconsistencies might be found. It is demonstrated that the common nonlinear static analysis (pushover analysis) cannot be used to assess the response of the rack and the behaviour factor selection from current standards could be unjustifiable. Also, common engineering demand parameters, e.g. interstorey drift ratio, need further assessment vis-à-vis the response of nonstructural components of which the current design method does not comply with modern methods.
Bisexuality, Union Status, and Gender Composition of the Couple: Reexamining Marital Advantage in Health
Authors: Hsieh Ning , Liu Hui
Published: September 2019

It has long been documented that married individuals have better health outcomes than unmarried individuals. However, this marital advantage paradigm has been developed primarily based on heterosexual populations. No studies to date have examined the health effects of marriage among bisexuals, one of the most disadvantaged but understudied sexual minority groups, although a few have shown mixed results for gays and lesbians. Similarly, no research has examined how the gender composition of a couple may shape bisexuals’ health outcomes above and beyond the effects of sexual orientation. We analyzed pooled data from the 2013–2017 National Health Interview Survey (n = 154,485) and found that the health advantage of marriage applied only to heterosexuals and, to a lesser extent, gays and lesbians. Married bisexuals, however, exhibited poorer health than unmarried bisexuals when socioeconomic status and health behaviors were adjusted for. Moreover, bisexuals in same-gender unions were healthier than bisexuals in different-gender unions primarily because of their socioeconomic advantages and healthier behaviors. Together, our findings suggest that bisexuals, particularly those in different-gender unions, face unique challenges in their relationships that may reduce the health advantage associated with marriage.
Accuracy of psychometric tools in the assessment of personality in
adolescents and adults requesting gender-affirming treatments: A systematic
review
Authors: Lehmann Katrin , Leavey Gerard
Published: September 2019

Background:

The assessment and screening for personality disorders in
individuals requesting gender affirming treatments may be an important
aspect of predicting medical and surgical outcomes for this population,
but there is no consensus on how best to do so.

Aims:

To review the diagnostic accuracy of psychometric tools used for the
assessment of personality disorders in those requesting gender affirming
treatments.

Method:

A systematic review: Prospero CRD42017078783 [1].

Results:

Many studies have focussed on the assessment of personality
disorders in this population, but since 1979, only two have used an
index and reference test.

Conclusion:

There are no agreed reference standards for this population and
psychometric tools continue to be scored on reference data from the
cisgender (not transgender) population. We need robust evidence on this
issue, as individuals may be denied access to gender affirming
treatments based on psychometric tools without established reliability
in this population.
Review on TNSA diagnostics and recent developments at SPARC_LAB
Authors: Bisesto Fabrizio , Galletti Mario , Anania Maria Pia , Ferrario Massimo , Pompili Riccardo , Botton Mordechai , Schleifer Elad , Zigler Arie
Published: September 2019

Interaction between high-intensity lasers with solid targets is the key process in a wide range of novel laser-based particle accelerator schemes, as well as electromagnetic radiation sources. Common to all the processes is the generation of femtosecond pulses of relativistic electrons emitted from the targets as forerunners of the later-time principal products of the interaction scheme. In this paper, some diagnostics employed in laser–solid matter interaction experiments related to electrons, protons, ions, electromagnetic pulses (EMPs) and X-rays are reviewed. Then, we present our experimental study regarding fast electrons and EMPs utilizing a femtosecond-resolution detector previously adopted only in accelerator facilities.
Experimental study of particle trajectories below deep-water surface gravity wave groups
Authors: van den Bremer T. S. , Whittaker C. , Calvert R. , Raby A. , Taylor P. H.
Published: September 2019

Owing to the interplay between the forward Stokes drift and the backward wave-induced Eulerian return flow, Lagrangian particles underneath surface gravity wave groups can follow different trajectories depending on their initial depth below the surface. The motion of particles near the free surface is dominated by the waves and their Stokes drift, whereas particles at large depths follow horseshoe-shaped trajectories dominated by the Eulerian return flow. For unidirectional wave groups, a small net displacement in the direction of travel of the group results near the surface, and is accompanied by a net particle displacement in the opposite direction at depth. For deep-water waves, we study these trajectories experimentally by means of particle tracking velocimetry in a two-dimensional flume. In doing so, we provide visual illustration of Lagrangian trajectories under groups, including the contributions of both the Stokes drift and the Eulerian return flow to both the horizontal and the vertical Lagrangian displacements. We compare our experimental results to leading-order solutions of the irrotational water wave equations, finding good agreement.
Crowdsourcing Reliable Local Data
Authors: Sumner Jane Lawrence , Farris Emily M. , Holman Mirya R.
Published: September 2019

The adage “All politics is local” in the United States is largely true. Of the United States’ 90,106 governments, 99.9% are local governments. Despite variations in institutional features, descriptive representation, and policy-making power, political scientists have been slow to take advantage of these variations. One obstacle is that comprehensive data on local politics is often extremely difficult to obtain; as a result, data is unavailable or costly, hard to replicate, and rarely updated. We provide an alternative: crowdsourcing this data. We demonstrate and validate crowdsourcing data on local politics using two different data collection projects. We evaluate different measures of consensus across coders and validate the crowd’s work against elite and professional datasets. In doing so, we show that crowdsourced data is both highly accurate and easy to use. In doing so, we demonstrate that nonexperts can be used to collect, validate, or update local data.
New Evidence for a Mid- to Late-Holocene Change in the Marine Reservoir Effect Across the South Pacific Gyre
Authors: Petchey Fiona
Published: September 2019

Holocene climate change in the South Pacific is of major interest to archaeologists and Quaternary researchers. Regional surface ocean radiocarbon (14C) values are an established proxy for studying changing oceanographic and climatic conditions. Unfortunately, radiocarbon variability in the marine environment over the period of specific importance to human colonization of the remote Pacific islands—the last 3500 years—has been poorly studied. In order to build robust and accurate archaeological chronologies using shell, it is important to rectify this. In this paper, radiocarbon marine reservoir offsets (ΔR) are presented from eight archaeological sites, ranging in age from 350 cal BP to 3000 cal BP, and compared to coral datasets from the east Australian coastline. The results indicate that a significant decrease in the South Pacific Gyre ΔR occurred between 2600 and 2250 cal BP, most likely caused by changes in ocean circulation and climate. Accurately recording the timing of variability in reservoir offset is critical to untangling changes in society that took place in the Pacific, in particular, the development of Ancestral Polynesian Society.
Exit Strategy: Career Concerns and Revolving Doors in Congress
Authors: SHEPHERD MICHAEL E. , YOU HYE YOUNG
Published: September 2019

Although the majority of research on revolving-door lobbyists centers on the influence they exercise during their postgovernment careers, relatively little attention is given to whether future career concerns affect the behaviors of revolving-door lobbyists while they still work in government. We argue that the revolving-door incentivizes congressional staffers to showcase their legislative skills to the lobbying market in ways that affect policymaking in Congress. Using comprehensive data on congressional staffers, we find that employing staffers who later become lobbyists is associated with higher legislative productivity for members of Congress, especially in staffers’ final terms in Congress. It also is associated with increases in a member’s bill sponsorship in the areas of health and commerce, the topics most frequently addressed by clients in the lobbying industry, as well as granting more access to lobbying firms. These results provide the systematic empirical evidence of pre-exit effects of the revolving-door among congressional staff.
THE GREEK ὝΜΝΟΣ: HIGH PRAISE FOR GODS AND MEN
Authors: Brumbaugh Michael E.
Published: September 2019

Over a hundred instances of the word ὕμνος from extant archaic poetry demonstrate that the Greek hymn was understood broadly as a song of praise. The majority of these instances comes from Pindar, who regularly uses the term to describe his poems celebrating athletic victors. Indeed, Pindar and his contemporaries saw the ὕμνος as a powerful vehicle for praising gods, heroes, men and their achievements—often in service of an ideological agenda. Writing a century later Plato used the term frequently and with much the same range. A survey of his usage reveals instances of ὕμνοι for gods,daimones, heroes, ancestors, leading citizens, noble deeds, sites and landscapes. Despite abundant evidence of Plato's own practice, studies of the Greek hymn posit an extreme narrowing of the genre in the classical period and cite the philosopher as the sole witness to, if not the originator of, this development. Two passages in particular, one from theRepublicand one from theLaws, are seen to support the claim that by the fourth centuryb.c.e.the term ὕμνος refers exclusively to songs for gods. InRepublicBook 10, we find the memorable edict on poetic censorship: ‘But we must know that of poetry only ὕμνοι for the gods and ἐγκώμια for the good must be admitted into our city.’LawsBook 3 offers what appears to be an even more straightforward pronouncement: ‘Back then our music was divided according to its various types and arrangements; and a certain type of song was prayers to the gods, and these were called by the name ὕμνοι.’ From these two statements has arisen the consensus that Plato saw a divine recipient as the defining feature of the ὕμνος and, moreover, that this position reflects thecommunis opiniofrom at least the fourth centuryb.c.e.onward.
LÉON WALRAS’S THEORY OF PUBLIC INTEREST GOODS: TOWARD AN ORGANIC VIEW OF THE STATE
Authors: Béraud Alain , Numa Guy
Published: September 2019

The purpose of this essay is to analyze Léon Walras’s theory of public interest goods. For him, “services and products of public interest are theoretically those that interest men as members of the community or of the State emanating from the authority to establish social conditions, that is, from the satisfaction of needs that are the same and equal to all” ([1875, 1897a] 1992, EEPA, p. 187). In Walras’s mind, this definition meant that public interest goods could not be factored into the utility function, in sharp contrast with the standard approach in public goods theory. Walras did not imply that public interest goods were not useful, but he maintained instead that their utility was felt only by the community as a whole and not by the individual. Walras developed an anti-individualistic view of the State in which the collective interest was not reducible to the sum of private interests.
TAME BLOCK ALGEBRAS OF HECKE ALGEBRAS OF CLASSICAL TYPE
Authors: ARIKI SUSUMU
Published: September 2019

We classify tame block algebras of Hecke algebras of classical type over an algebraically closed field of characteristic not equal to two.
Design of subwavelength metamaterial resonator based on new self-coupling strategy
Authors: Benkhaoua Larbi , Mouissat Smail , Benhabiles Mohamed Taoufik , Riabi Mohamed Lahdi
Published: September 2019

In this paper, edge-broad-side coupled spiral resonator (EBC-SR) is proposed to enhance the miniaturization of the metamaterial resonators. After a comparative analysis, it is found that the proposed strategy can drastically reduce the electrical size of metamaterial resonators. This is due to the use, at the same time, advantages of edge coupling, broad-side coupling, and self-coupling. A lumped element equivalent circuit model is proposed for the EBC-SR-loaded transmission line. This model is validated by comparing the results of electromagnetic simulations with the circuit simulations using the extracted parameter values. To validate the proposed strategy, a prototype of the EBC-SR embedded inside a drive loop is fabricated. The experimental results are presented with the simulation results.
Psychometrical Properties of a French Version of the General Self-Efficacy Short Scale (ASKU)
Authors: Décieux Jean Philippe , Sischka Philipp Emanuel , Schumacher Anette , Willems Helmut
Published: September 2019

Abstract. General self-efficacy is a central personality trait often evaluated in surveys as context variable. It can be interpreted as a personal coping resource reflecting individual belief in one’s overall competence to perform across a variety of situations. The German-language Allgemeine-Selbstwirksamkeit-Kurzskala (ASKU) is a reliable and valid instrument to assess this disposition in the German-speaking countries based on a three-item equation. This study develops a French version of the ASKU and tests this French version for measurement invariance compared to the original ASKU. A reliable and valid French instrument would make it easy to collect data in the French-speaking countries and allow comparisons between the French and German results. Data were collected on a sample of 1,716 adolescents. Confirmatory factor analysis resulted in a good fit for a single-factor model of the data (in total, French, and German version). Additionally, construct validity was assessed by elucidating intercorrelations between the ASKU and different factors that should theoretically be related to ASKU. Furthermore, we confirmed configural and metric as well as scalar invariance between the different language versions, meaning that all forms of statistical comparison between the developed French version and the original German version are allowed.
We Only Believe in News That We Doctored Ourselves
Authors: Faragó Laura , Kende Anna , Krekó Péter
Published: September 2019

Abstract. In this research we aimed to explore the importance of partisanship behind the belief in wish-fulfilling political fake news. We tested the role of political orientation, partisanship, and conspiracy mentality in the acceptance of pro- and anti-government pipedream fake news. Using a representative survey ( N = 1,000) and a student sample ( N = 382) in Hungary, we found that partisanship predicted belief in political fake news more strongly than conspiracy mentality, and these connections were mediated by the perceived credibility of source (independent journalism vs. political propaganda) and economic sentiment. Our findings suggest that political bias can be more important in predicting acceptance of pipedream political fake news than conspiracy mentality.
Youth Activists Call for Urgent Climate Action
Authors: Showstack Randy
Published: September 2019

Greta Thunberg and other youth climate activists came to Washington, D.C., days before a major United Nations conference to draw attention to the need for immediate action to address climate change.
A long noncoding RNA binding to QKI-5 regulates germ cell apoptosis via p38 MAPK signaling pathway
Authors: Li Kai , Zhong Shunshun , Luo Yanyun , Zou Dingfeng , Li Mengzhen , Li Yahui , Lu Yan , Miao Shiying , Wang Linfang , Song Wei
Published: September 2019

Spermatogenesis is the complex process of male germline development and requires coordinated interactions by multiple gene products that undergo strict developmental regulations. Increasing evidence has suggested that a number of long noncoding RNAs (lncRNAs) may function as important regulatory molecules in various physiological and pathological processes by binding to specific proteins. Here, we identified a subset of QKI-5-binding lncRNAs in the mouse testis through the integrated analyses of RNA immunoprecipitation (RIP)-microarray and biological verification. Among the lncRNAs, we revealed that NONMMUT074098.2 (Lnc10), which was highly expressed in the spermatogonia and spermatocytes of the testis, interacted with QKI-5. Furthermore, Lnc10 depletion promoted germ cell apoptosis via the activation of p38 MAPK, whereas the simultaneous knockdown of QKI-5 could rescue the apoptotic phenotype and the activation of p38 MAPK, which were induced by the loss of Lnc10. These data indicated that the Lnc10-QKI-5 interaction was associated with the regulatory roles of QKI-5 and that the Lnc10-QKI-5 interaction inhibited the regulation of QKI-5 on the downstream p38 MAPK signaling pathway. Additionally, we functionally characterized the biological roles of Lnc10 and found that the knockdown of Lnc10 promoted the apoptosis of spermatogenic cells in vivo; this suggested that Lnc10 had an important biological role in mouse spermatogenesis. Thus, our study provides a potential strategy to investigate the biological significance of lncRNA-RBP interactions during male germline development.
Uprooting defects to enable high-performance III–V optoelectronic devices on silicon
Authors: Bioud Youcef A. , Boucherif Abderraouf , Myronov Maksym , Soltani Ali , Patriarche Gilles , Braidy Nadi , Jellite Mourad , Drouin Dominique , Arès Richard
Published: September 2019

The monolithic integration of III-V compound semiconductor devices with silicon presents physical and technological challenges, linked to the creation of defects during the deposition process. Herein, a new defect elimination strategy in highly mismatched heteroepitaxy is demonstrated to achieve a ultra-low dislocation density, epi-ready Ge/Si virtual substrate on a wafer scale, using a highly scalable process. Dislocations are eliminated from the epilayer through dislocation-selective electrochemical deep etching followed by thermal annealing, which creates nanovoids that attract dislocations, facilitating their subsequent annihilation. The averaged dislocation density is reduced by over three orders of magnitude, from ~108 cm−2 to a lower-limit of ~104 cm−2 for 1.5 µm thick Ge layer. The optical properties indicate a strong enhancement of luminescence efficiency in GaAs grown on this virtual substrate. Collectively, this work demonstrates the promise for transfer of this technology to industrial-scale production of integrated photonic and optoelectronic devices on Si platforms in a cost-effective way.
A Mendelian randomization study of IL6 signaling in cardiovascular diseases, immune-related disorders and longevity
Authors: Rosa Mickael , Chignon Arnaud , Li Zhonglin , Boulanger Marie-Chloé , Arsenault Benoit J. , Bossé Yohan , Thériault Sébastien , Mathieu Patrick
Published: September 2019

Growing evidence suggests that inflammation is a significant contributor to different cardiovascular diseases (CVDs). Mendelian randomization (MR) was performed to assess the causal inference between plasma soluble IL6 receptor (sIL6R), a negative regulator of IL6 signaling, and different cardiovascular and immune-related disorders.Cis-MR with multiple instrumental variables showed an inverse association of sIL6R with rheumatoid arthritis, atrial fibrillation, stroke, coronary artery disease, and abdominal aortic aneurysm. However, genetically-determined sIL6R level was positively associated with atopic dermatitis and asthma. Also, sIL6R level was associated with longevity, as evaluated by parental age at death, a heritable trait. Gene-based association analysis with S-PrediXcan by using tissues from GTExV7 showed thatIL6Rtissue expression-disease pair associations were consistent with the directional effect of IL6 signaling identified in MR. Genetically-determined reduced IL6 signaling lowers the risk of multiple CVDs and is associated with increased longevity, but at the expense of higher atopic risk.
Digital quantum simulation, Trotter errors, and quantum chaos of the kicked top
Authors: Sieberer Lukas M. , Olsacher Tobias , Elben Andreas , Heyl Markus , Hauke Philipp , Haake Fritz , Zoller Peter
Published: September 2019

This work aims at giving Trotter errors in digital quantum simulation (DQS) of collective spin systems an interpretation in terms of quantum chaos of the kicked top. In particular, for DQS of such systems, regular dynamics of the kicked top ensures convergence of the Trotterized time evolution, while chaos in the top, which sets in above a sharp threshold value of the Trotter step size, corresponds to the proliferation of Trotter errors. We show the possibility to analyze this phenomenology in a wide variety of experimental realizations of the kicked top, ranging from single atomic spins to trapped-ion quantum simulators which implement DQS of all-to-all interacting spin-1/2 systems. These platforms thus enable in-depth studies of Trotter errors and their relation to signatures of quantum chaos, including the growth of out-of-time-ordered correlators.
Evaluating FAIR maturity through a scalable, automated, community-governed framework
Authors: Wilkinson Mark D. , Dumontier Michel , Sansone Susanna-Assunta , Bonino da Silva Santos Luiz Olavo , Prieto Mario , Batista Dominique , McQuilton Peter , Kuhn Tobias , Rocca-Serra Philippe , Crosas Mercѐ , Schultes Erik
Published: September 2019

Transparent evaluations of FAIRness are increasingly required by a wide range of stakeholders, from scientists to publishers, funding agencies and policy makers. We propose a scalable, automatable framework to evaluate digital resources that encompasses measurable indicators, open source tools, and participation guidelines, which come together to accommodate domain relevant community-defined FAIR assessments. The components of the framework are: (1) Maturity Indicators – community-authored specifications that delimit a specific automatically-measurable FAIR behavior; (2) Compliance Tests – small Web apps that test digital resources against individual Maturity Indicators; and (3) the Evaluator, a Web application that registers, assembles, and applies community-relevant sets of Compliance Tests against a digital resource, and provides a detailed report about what a machine “sees” when it visits that resource. We discuss the technical and social considerations of FAIR assessments, and how this translates to our community-driven infrastructure. We then illustrate how the output of the Evaluator tool can serve as a roadmap to assist data stewards to incrementally and realistically improve the FAIRness of their resources.
All-silicon reconfigurable metasurfaces for multifunction and tunable performance at optical frequencies based on glide symmetry
Authors: Shanei Mohammad Mahdi , Fathi Davood , Ghasemifard Fatemeh , Quevedo-Teruel Oscar
Published: September 2019

Dielectric metasurfaces have opened promising possibilities to enable a versatile platform in the miniaturization of optical elements at visible and infrared frequencies. Due to high efficiency and compatibility with CMOS fabrication technology, silicon-based metasurfaces have a remarkable potential for a wide variety of optical devices. Adding tunability mechanisms to metasurfaces could be beneficial for their application in areas such as communications, imaging and sensing. In this paper, we propose an all-silicon reconfigurable metasurface based on the concept of glide symmetry. The reconfigurability is achieved by a phase modulation of the transmitted wave activated by a lateral displacement of the layers. The misalignment between the layers creates a new inner periodicity which leads to the formation of a metamolecule with a new sort of near-field interaction. The proposed approach is highly versatile for developing multifunctional and tunable metadevices at optical frequencies. As a proof of concept, in this paper, we design a bifunctional metadevice, as well as a tunable lens and a controllable beam deflector operating at 1.55 μm.
Pioglitazone treatment prior to transplantation improves the efficacy of human mesenchymal stem cells after traumatic brain injury in rats
Authors: Das Mahasweta , Mayilsamy Karthick , Tang Xiaolan , Han Jung Yeon , Foran Elspeth , Willing Alison E. , Mohapatra Shyam S. , Mohapatra Subhra
Published: September 2019

Traumatic brain injury is a leading cause of death and disability around the world. So far, drugs are not available to repair brain damage. Human mesenchymal stem cell (hMSC) transplantation therapy is a promising approach, although the inflammatory microenvironment of the injured brain affects the efficacy of transplanted hMSCs. We hypothesize that reducing the inflammation in the cerebral microenvironment by reducing pro-inflammatory chemokines prior to hMSC administration will improve the efficacy of hMSC therapy. In a rat model of lateral fluid percussion injury, combined pioglitazone (PG) and hMSC (combination) treatment showed less anxiety-like behavior and improved sensorimotor responses to a noxious cold stimulus. Significant reduction in brain lesion volume, neurodegeneration, microgliosis and astrogliosis were observed after combination treatment. TBI induced expression of inflammatory chemokine CCL20 and IL1-β were significantly decreased in the combination treatment group. Combination treatment significantly increased brain-derived neurotrophic factor (BDNF) level and subventricular zone (SVZ) neurogenesis. Taken together, reducing proinflammatory cytokine expression in the cerebral tissues after TBI by PG administration and prior to hMSC therapy improves the outcome of the therapy in which BDNF could have a role.
Inflammation markers in the saliva of infants born from Zika-infected mothers: exploring potential mechanisms of microcephaly during fetal development
Authors: de Oliveira Diogo N. , Lima Estela O. , Melo Carlos F. O. R. , Delafiori Jeany , Guerreiro Tatiane M. , Rodrigues Rafael G. M. , Morishita Karen N. , Silveira Cynthia , Muraro Stéfanie Primon , de Souza Gabriela Fabiano , Vieira Aline , Silva Antônio , Batista Rosângela F. , Doriqui Maria J. R. , Sousa Patricia S. , Milanez Guilherme P. , Proença-Módena José L. , Cavalcanti Denise P. , Catharino Rodrigo R.
Published: September 2019

Zika virus (ZIKV) has emerged as one of the most medically relevant viral infections of the past decades; the devastating effects of this virus over the developing brain are a major matter of concern during pregnancy. Although the connection with congenital malformations are well documented, the mechanisms by which ZIKV reach the central nervous system (CNS) and the causes of impaired cortical growth in affected fetuses need to be better addressed. We performed a non-invasive, metabolomics-based screening of saliva from infants with congenital Zika syndrome (CZS), born from mothers that were infected with ZIKV during pregnancy. We were able to identify three biomarkers that suggest that this population suffered from an important inflammatory process; with the detection of mediators associated with glial activation, we propose that microcephaly is a product of immune response to the virus, as well as excitotoxicity mechanisms, which remain ongoing even after birth.
Identification and validation of potential reference gene for effective dsRNA knockdown analysis in Chilo partellus
Authors: Adeyinka Olawale Samuel , Tabassum Bushra , Nasir Idrees Ahmad , Yousaf Iqra , Sajid Imtiaz Ahmad , Shehzad Khurram , Batcho Anicet , Husnain Tayyab
Published: September 2019

Chilo partellus is an invasive polyphagous pest that has not been effectively managed with chemical pesticides. To select potential dsRNAs for use in an alternate control strategy, it is crucial to identify and evaluate stable reference genes for knockdown expression studies. This study evaluates the expression stability of seven candidate reference genes in C. partellus larvae fed on crude bacterially-expressed dsRNAs and purified dsRNAs at different time intervals, as well as the developmental stages and sexes. The expression stabilities of the reference genes were evaluated with different software programmes, such as BestKeeper, NormFinder, deltaCt, geNorm, and RefFinder. The overall results rank ELF as the most stably expressed reference gene when larvae were fed with crude bacteria-induced dsRNAs and purified dsRNA. However, Tubulin and HSP70 were more stable under different developmental stages and sexes. The expression levels of larvae that were fed crude bacteria-induced dsRNAs of Chitinase and Acetylcholinesterase were normalized with the four most stable reference genes (ELF, HSP70, V-ATPase and Tubulin) and the least stable reference gene (18S and HSP70) based on the geNorm algorithm. The least stable reference gene showed inconsistent knockdown expression, thereby confirming that the validation of a suitable reference gene is crucial to improve assay accuracy for dsRNA-targeted gene selection in C. partellus.
Comparative analysis of the fecal microbiota from different species of domesticated and wild suids
Authors: Correa-Fiz Florencia , Blanco-Fuertes Miguel , Navas Maria J. , Lacasta Anna , Bishop Richard P. , Githaka Naftaly , Onzere Cynthia , Le Potier Marie-Frédérique , Almagro-Delgado Vanessa , Martinez Jorge , Aragon Virginia , Rodriguez Fernando
Published: September 2019

Most of the microorganisms living in a symbiotic relationship in different animal body sites (microbiota) reside in the gastrointestinal tract (GIT). Several studies have shown that the microbiota is involved in host susceptibilities to pathogens. The fecal microbiota of domestic and wild suids was analyzed. Bacterial communities were determined from feces obtained from domestic pigs (Sus scrofa) raised under different conditions: specific-pathogen-free (SPF) pigs and domestic pigs from the same bred, and indigenous domestic pigs from a backyard farm in Kenya. Secondly, the fecal microbiota composition of the African swine fever (ASF) resistant warthogs (Phacochoerus africanus) from Africa and a European zoo was determined. African swine fever (ASF) is a devastating disease for domestic pigs. African animals showed the highest microbial diversity while the SPF pigs the lowest. Analysis of the core microbiota from warthogs (resistant to ASF) and pigs (susceptible to ASF) showed 45 shared OTUs, while 6 OTUs were exclusively present in resistant animals. These six OTUs were members of the Moraxellaceae family, Pseudomonadales order and Paludibacter, Anaeroplasma, Petrimonas, and Moraxella genera. Further characterization of these microbial communities should be performed to determine the potential involvement in ASF resistance.
A Cross-Sectional Study on the Dietary Pattern Impact on Cardiovascular Disease Biomarkers in Malaysia
Authors: Karupaiah Tilakavati , Chuah Khun-Aik , Chinna Karuthan , Pressman Peter , Clemens Roger A. , Hayes A. Wallace , Sundram Kalyana
Published: September 2019

We conducted this cross-sectional population study with a healthy multi-ethnic urban population (n = 577) in Malaysia, combining nutritional assessments with cardiometabolic biomarkers defined by lipid, atherogenic lipoproteins, inflammation and insulin resistance. We found diametrically opposing associations of carbohydrate (246·6 ± 57·7 g, 54·3 ± 6·5%-TEI) and fat (total = 64·5 ± 19·8 g, 31·6 ± 5·5%-TEI; saturated fat = 14·1 ± 2·7%-TEI) intakes as regards waist circumference, HDL-C, blood pressure, glucose, insulin and HOMA2-IR as well as the large-LDL and large-HDL lipoprotein particles. Diets were then differentiated into either low fat (LF, <30% TEI or <50 g) or high fat (HF, >35% TEI or >70 g) and low carbohydrate (LC, <210 g) or high carbohydrate (HC, >285 g) which yielded LFLC, LFHC, HFLC and HFHC groupings. Cardiometabolic biomarkers were not significantly different (P > 0.05) between LFLC and HFLC groups. LFLC had significantly higher large-LDL particle concentrations compared to HFHC. HOMA-IR2 was significantly higher with HFHC (1·91 ± 1·85, P < 0·001) versus other fat-carbohydrate combinations (LFLC = 1·34 ± 1·07, HFLC = 1·41 ± 1·07; LFHC = 1·31 ± 0·93). After co-variate adjustment, odds of having HOMA2-IR >1.7 in the HFHC group was 2.43 (95% CI: 1·03, 5·72) times more compared to LFLC while odds of having large-LDL <450 nmol/L in the HFHC group was 1.91 (95% CI: 1·06, 3·44) more compared to latter group. Our data suggests that a HFHC dietary combination in Malaysian adults is associated with significant impact on lipoprotein particles and insulin resistance.
Enhancing innate antiviral immune responses in rainbow trout by double stranded RNA delivered with cationic phytoglycogen nanoparticles
Authors: Alkie Tamiru N. , de Jong Jondavid , Jenik Kristof , Klinger Karl M. , DeWitte-Orr Stephanie J.
Published: September 2019

Innate immunity is induced when pathogen-associated molecular patterns (PAMPs) bind host pattern recognition receptors (PRRs). Polyinosinic:polycytidylic acid [poly(I:C)] is a synthetic analogue of viral dsRNA that acts as a PAMP, inducing type I interferons (IFNs) in vertebrates. In the present study, the immunostimulatory effects of high molecular weight (HMW) poly(I:C) in rainbow trout cells were measured when bound to a cationic phytoglycogen nanoparticle (Nano-HMW). The physical characteristics of the nanoparticle itself, when bound to different lengths of dsRNA and when cell associated was evaluated. Optimal concentration and timing for innate immune stimulation was measured using the RTG-P1 reporter cell line. The immunostimulatory effects of HMW poly (I:C) was compared to Nano-HMW in vitro using the RTgutGC cell line cultured in a conventional monolayer or a transwell culture system. The ability of an activated intestinal epithelium to transmit an antiviral signal to macrophages was evaluated using a co-culture of RTgutGC cells and RTSll (a monocyte/macrophage cell). In all culture conditions, Nano-HMW was a more effective inducer of IFN-related antiviral immune responses compared to HMW poly (I:C) alone. This study introduces the use of cationic phytoglycogen nanoparticles as a novel delivery system for immunomodulatory molecules to enhance immune responses in aquatic vertebrates.
Structure of full-length wild-type human phenylalanine hydroxylase by small angle X-ray scattering reveals substrate-induced conformational stability
Authors: Tomé Catarina S. , Lopes Raquel R. , Sousa Pedro M. F. , Amaro Mariana P. , Leandro João , Mertens Haydyn D. T. , Leandro Paula , Vicente João B.
Published: September 2019

Human phenylalanine hydroxylase (hPAH) hydroxylates l-phenylalanine (l-Phe) to l-tyrosine, a precursor for neurotransmitter biosynthesis. Phenylketonuria (PKU), caused by mutations in PAH that impair PAH function, leads to neurological impairment when untreated. Understanding the hPAH structural and regulatory properties is essential to outline PKU pathophysiological mechanisms. Each hPAH monomer comprises an N-terminal regulatory, a central catalytic and a C-terminal oligomerisation domain. To maintain physiological l-Phe levels, hPAH employs complex regulatory mechanisms. Resting PAH adopts an auto-inhibited conformation where regulatory domains block access to the active site. l-Phe-mediated allosteric activation induces a repositioning of the regulatory domains. Since a structure of activated wild-type hPAH is lacking, we addressed hPAH l-Phe-mediated conformational changes and report the first solution structure of the allosterically activated state. Our solution structures obtained by small-angle X-ray scattering support a tetramer with distorted P222 symmetry, where catalytic and oligomerisation domains form a core from which regulatory domains protrude, positioning themselves close to the active site entrance in the absence of l-Phe. Binding of l-Phe induces a large movement and dimerisation of regulatory domains, exposing the active site. Activated hPAH is more resistant to proteolytic cleavage and thermal denaturation, suggesting that the association of regulatory domains stabilises hPAH.