bluuebunny/crossref_metadata_2025 huggingface dataset:
A health terminological system for inherited retinal diseases: Content coverage evaluation and a proposed classification
Authors: Sabbaghi Hamideh , Madani Sina , Ahmadieh Hamid , Daftarian Narsis , Suri Fatemeh , Khorrami Farid , Saviz Proshat , Shahriari Mohammad Hasan , Motevasseli Tahmineh , Fekri Sahba , Nourinia Ramin , Moradian Siamak , Sheikhtaheri Abbas
Published: August 2023

Purpose

To present a classification of inherited retinal diseases (IRDs) and evaluate its content coverage in comparison with common standard terminology systems.

Methods

In this comparative cross-sectional study, a panel of subject matter experts annotated a list of IRDs based on a comprehensive review of the literature. Then, they leveraged clinical terminologies from various reference sets including Unified Medical Language System (UMLS), Online Mendelian Inheritance in Man (OMIM), International Classification of Diseases (ICD-11), Systematized Nomenclature of Medicine (SNOMED-CT) and Orphanet Rare Disease Ontology (ORDO).

Results

Initially, we generated a hierarchical classification of 62 IRD diagnosis concepts in six categories. Subsequently, the classification was extended to 164 IRD diagnoses after adding concepts from various standard terminologies. Finally, 158 concepts were selected to be classified into six categories and genetic subtypes of 412 cases were added to the related concepts. UMLS has the greatest content coverage of 90.51% followed respectively by SNOMED-CT (83.54%), ORDO (81.01%), OMIM (60.76%), and ICD-11 (60.13%). There were 53 IRD concepts (33.54%) that were covered by all five investigated systems. However, 2.53% of the IRD concepts in our classification were not covered by any of the standard terminologies.

Conclusions

This comprehensive classification system was established to organize IRD diseases based on phenotypic and genotypic specifications. It could potentially be used for IRD clinical documentation purposes and could also be considered a preliminary step forward to developing a more robust standard ontology for IRDs or updating available standard terminologies. In comparison, the greatest content coverage of our proposed classification was related to the UMLS Metathesaurus.
Increased frequency and quantity of mucosal and plasma cytomegalovirus replication among Ugandan Adults Living with HIV
Authors: McClymont Elisabeth , Bone Jeffrey , Orem Jackson , Okuku Fred , Kalinaki Mary , Saracino Misty , Huang Meei-Li , Selke Stacy , Wald Anna , Corey Lawrence , Casper Corey , Boucoiran Isabelle , Johnston Christine , Gantt Soren
Published: August 2023

Background

Co-infection with HIV can result in impaired control of cytomegalovirus (CMV) replication, increasing the likelihood of disease and onward transmission. The objective of this analysis was to measure the impact of HIV on CMV replication in an intensively-sampled cohort in Kampala, Uganda.

Methods

CMV seropositive men and women aged 18–65, with or without HIV co-infection, were followed for one month. Daily oral swabs and weekly anogenital swabs and plasma were collected. Quantitative CMV PCR was performed on all samples.

Results

Eighty-five participants were enrolled and provided ≥1 oral swab; 43 (51%) were HIV-seropositive. People living with HIV (PLWH; median CD4 count 439 cells/mm3; none on antiretrovirals) had 2–4 times greater risk of CMV detection at each anatomical site assessed. At the oral site, 773 of 1272 (61%) of samples from PLWH had CMV detected, compared to 214 of 1349 (16%) among people without HIV. Similarly, the mean CMV quantity was higher among PLWH at all anatomical sites, with the largest difference seen for oral swabs (mean difference 1.63 log/mL; 95% CI 1.13–2.13). Among PLWH, absolute quantity of CD4+ T-cells was not associated with risk of CMV detection. HIV plasma RNA quantity was positively correlated with oral CMV shedding frequency, but not detection at other sites.

Conclusions

Mucosal and systemic CMV replication occurs at higher levels in PLWH than people without HIV, particularly oral shedding, which is a major mode of CMV transmission. Increased CMV replication despite relatively preserved CD4+ T-cell counts suggests that additional interventions are required to improve CMV control in PLWH.
Supporting the wellbeing of caregivers of children on the autism spectrum: A qualitative report on experiences of attending group dance movement psychotherapy
Authors: Aithal Supritha , Karkou Vicky , Makris Stergios , Karaminis Themis , Powell Joanne
Published: August 2023

Caregivers of children on the autism spectrum can carry a significant amount of practical, psychological, and social demands and responsibilities that are highly stressful. A group Dance Movement Psychotherapy (DMP) was offered to facilitate the wellbeing of caregivers. In this article, we explore the experiences of the therapeutic processes and outcomes of the intervention from the perspectives of caregivers, the therapist, and the researcher/co-facilitator. Method: Four clusters of caregivers of children on the autism spectrum (N = 20 Mean age = 39.25 years) took part in five group DMP sessions lasting 90 minutes delivered across two special educational needs settings. Twenty reflective focus groups took place in total, with each taking place at the end of each DMP session. Participants were invited to capture their experiences through arts-based drawings, while therapist and participating researcher/co-facilitator kept session-based notes and arts-based reflections. These arts-based and verbal data were grouped to generate themes. Results: Six overarching themes emerged from the arts-based and verbal data with multiple subthemes that describe the contribution of DMP towards promoting caregivers’ wellbeing and identified key challenges in implementing the intervention. These themes are: (1) Beholding within and around; (2) Reflecting and reinforcing strengths; (3) Exchanging views; (4) Looking back and carrying forward; (5) Core benefits; and (6) Challenges to engage in DMP. Conclusion: Caregivers talked about their experience of participating in the DMP groups as positive and acknowledged the helpful and challenging aspects of taking part in DMP intervention. They appreciated the creative and expressive nature of the intervention to promote their emotional and social wellbeing. The challenges identified in the study indicate that further awareness is needed within school environments about the contribution arts therapies can make towards establishing appropriate and sustainable interventions for caregivers.
Testing the mutant selection window hypothesis with meropenem: In vitro model study with OXA-48-producing Klebsiella pneumoniae
Authors: Alieva Kamilla N. , Golikova Maria V. , Dovzhenko Svetlana A. , Kobrin Mikhail B. , Strukova Elena N. , Ageevets Vladimir A. , Avdeeva Alisa A. , Sulian Ofeliia S. , Sidorenko Sergey V. , Zinner Stephen H.
Published: August 2023

OXA-48 carbapenemases are frequently expressed by Klebsiella pneumoniae clinical isolates; they decrease the effectiveness of carbapenem therapy, particularly with meropenem. Among these isolates, meropenem-susceptible carbapenemase-producers may show decreased meropenem effectiveness. However, the probability of the emergence of resistance in susceptible carbapenemase-producing isolates and its dependence on specific K. pneumoniae meropenem MICs is not completely known. It is also not completely clear what resistance patterns will be exhibited by these bacteria exposed to meropenem, if they would follow the patterns of non-beta-lactamase-producing bacteria and other than beta-lactams antibiotics. These issues might be clarified if patterns of meropenem resistance related to the mutant selection window (MSW) hypothesis. To test the applicability of the MSW hypothesis to meropenem, OXA-48-carbapenemase-producing K. pneumoniae clinical isolates with MICs in a 64-fold range (from susceptible to resistant) were exposed to meropenem in a hollow-fiber infection model; epithelial lining fluid meropenem pharmacokinetics were simulated following administration of 2 grams every 8 hours in a 3-hour infusion. Strong bell-shaped relationships between the meropenem daily dose infused to the model as related to the specific isolate MIC and both the antimicrobial effect and the emergence of resistance were observed. The applicability of the MSW hypothesis to meropenem and carbapenemase producing K. pneumoniae was confirmed. Low meropenem efficacy indicates very careful prescribing of meropenem to treat K. pneumoniae infections when the causative isolate is confirmed as an OXA-48-carbapenemase producer.
Context-dependent memory recall in HMD-based immersive virtual environments
Authors: Chocholáčková Mária , Juřík Vojtěch , Ružičková Alexandra , Jurkovičová Lenka , Ugwitz Pavel , Jelínek Martin
Published: August 2023

The article introduces an original VR-based experiment which explores context-dependent memory recall in humans. It specifically examines the recall of correct and falsely induced semantic memories. With the aid of VR head-mounted displays, 92 students of psychology were placed in a computer-generated indoor virtual environment and asked to memorize the presented lists of words. Afterwards, the participants were placed in the same indoor virtual environment or an alternative outdoor virtual environment and asked to recall the words. The number of correct and falsely induced words was then measured. On average, women recalled significantly more correct words from the list than men, regardless of the environmental context. Despite the assumptions, we did not observe a separate effect of exposure to different environments during learning and recall of material on memory performance. Likewise, we did not detect any effects of the learning context or biological sex in the case of the production of false memories. These results provide a novel insight into previous knowledge regarding the memory processes that occur in virtual environments. Although we failed to confirm the role of context in recalling learned material in general, we found a hint that this context might interact with specific memory processes of biological sexes. However, the design of this study only captured the effect of changing the environment during memory recall and did not address the role of specific context in remembering learning material. Further research is therefore needed to better investigate these phenomena and examine the role of biological sex in context-dependent memory processes.
Brownfield land and health: A systematic review of the literature
Authors: Wang Weiyi , Dack Sarah , Mudway Ian , Walder Holly , Davies Bethan , Kamanyire Robie , Fecht Daniela
Published: August 2023

Background

Brownfield land is vacant or derelict land that was previously used for industrial or commercial purposes. Brownfield land is increasingly being targeted for housing development, however, depending on the previous use and remediation activity, it might pose potential risks to the health of residents on or in the vicinity of redeveloped sites. This systematic review of the literature synthesises the empirical evidence on the associations between brownfield land and health.

Methods

We systematically searched EMBASE, MEDLINE, Global Health, Web of Science, Scopus and GreenFile using a study protocol registered on PROSPERO (CRD42022286826). The search strategy combined the keywords “brownfield” and its interchangeable terms such as “previously developed land”, and any health outcomes such as “respiratory diseases” and “mortality”. Publications identified from the search were screened for eligibility by two authors, and data were extracted from the selected articles. Study quality was assessed based on the Newcastle-Ottawa Scale.

Results

Of the 1,987 records retrieved, 6 studies met the inclusion criteria; 3 ecological studies, 2 cross-sectional studies, and 1 longitudinal study. There was considerable heterogeneity in the exposure metrics and health outcomes assessed. All studies found significant positive associations between brownfield land proximity or density with at least one health relevant outcome, including poorer self-reported general health, increased mortality rates, increased birth defects, increased serum metal levels, and accelerated immune ageing.

Conclusions

Brownfield land may negatively affect the health of nearby residents. The epidemiological evidence on health effects associated with brownfield land in local communities, however, remains inconclusive and limited. Further studies are required to build the evidence base to inform future housing policies and urban planning.
Diabetes diminishes muscle precursor cell-mediated microvascular angiogenesis
Authors: Acosta Francisca M. , Pacelli Settimio , Rathbone Christopher R.
Published: August 2023

The skeletal muscles of Type II diabetic (T2D) patients can be characterized by a reduced vessel density, corresponding to deficiencies in microvascular angiogenesis. Interestingly, T2D also inhibits the function of many myogenic cells resident within skeletal muscle, including satellite cells, which are well-known for the role they play in maintaining homeostasis. The current study was undertaken to gain a better understanding of the mechanisms whereby satellite cell progeny, muscle precursor cells (MPCs), influence microvascular angiogenesis. Network growth and the expression of genes associated with angiogenesis were reduced when microvessels were treated with conditioned media generated by proliferating MPCs isolated from diabetic, as compared to control rat skeletal muscle, a phenomenon that was also observed when myoblasts from control or diabetic human skeletal muscle were used. When only exosomes derived from diabetic or control MPCs were used to treat microvessels, no differences in microvascular growth were observed. An evaluation of the angiogenesis factors in control and diabetic MPCs revealed differences in Leptin, vascular endothelial growth factor (VEGF), IL1-β, interleukin 10, and IP-10, and an evaluation of the MPC secretome revealed differences in interleukin 6, MCP-1, VEGF, and interleukin 4 exist. Angiogenesis was also reduced in tissue-engineered skeletal muscles (TE-SkM) containing microvessels when they were generated from MPCs isolated from diabetic as compared to control skeletal muscle. Lastly, the secretome of injured control, but not diabetic, TE-SkM was able to increase VEGF and increase microvascular angiogenesis. This comprehensive analysis of the interaction between MPCs and microvessels in the context of diabetes points to an area for alleviating the deleterious effects of diabetes on skeletal muscle.
Improved USER cloning for TALE assembly and its application to base editing
Authors: Zhou Jizeng , Wang Jiaowei , Chen Fangbing , Zhuang Zhenpeng , Chen Min , Yang Yang , Luo Xian , Tang Chengcheng , Zhou Xiaoqing , Chi Yue , Wang Jinling , He Yu , Zhang Kun , Zou Qingjian
Published: August 2023

Transcription activator-like effectors (TALEs) have been widely used for genome editing, transcriptional regulation, and locus-specific DNA imaging. However, TALEs are difficult to handle in routine laboratories because of their complexity and the considerable time consumed in TALE construction. Here, we described a simple and rapid TALE assembly method based on uracil-specific excision reagent (USER) cloning. Polymerase chain reaction was amplified with TALE trimer templates and deoxyuridine-containing primers. The products were treated with USER at 37°C for 30 min, followed by the treatment of T4 DNA Ligase at 16°C for 30 min. The TALE trimer unit could be rejoined hierarchically to form complete TALE expression vectors with high efficiency. This method was adopted to construct TALE-deaminases, which were used in combination with Cas9 nickases to generate efficient C-to-T or A-to-G base editing while eliminating predictable DNA off-target effects. This improved USER assembly is a simple, rapid, and laboratory-friendly TALE construction technique that will be valuable for DNA targeting.
Response of the competitive balance model to the external field
Authors: Oloomi Farideh , Kargaran Amir , Hosseiny Ali , Jafari Gholamreza
Published: August 2023

The competitive balance model was proposed as an extension of the structural balance theory, aiming to account for heterogeneities observed in real-world networks. In this model, different paradigms lead to form different friendship and enmity. As an example, friendship or enmity between countries can have a political or religious basis. The suggested Hamiltonian is symmetrical between paradigms. Our analyses show that a balanced state can be achieved if just one paradigm prevails in the network and the paradigm shift is possible only by imposing an external field. In this paper, we investigate the influence of the external field on the evolution of the network. We drive the mean-field solutions of the model and verify the accuracy of our analytical solutions by performing Monte-Carlo simulations. We observe that the external field breaks the symmetry of the system. The response of the system to this external field, contingent upon temperature, can be either paramagnetic or ferromagnetic. We observed a hysteresis behavior in the ferromagnetic regime. Once communities are formed based on a certain paradigm, then they resist change. We found that to avoid wasting energy we need to know the level of stochastic behavior in the network. Analogous to magnetic systems, we observe that susceptibility adheres to Curie’s law.
Arthroscopically assisted internal fixation for treatment of acute ankle fracture: A systematic review and meta-analysis of comparative studies
Authors: Zhuang Chen , Guo Wenxuan , Chen Wenhuan , Pan Yu , Zhuang Rujie
Published: August 2023

Background

Arthroscopically assisted reduction and internal fixation (ARIF) allows for the assessment of joint congruity following anatomic reduction, identification of occult intra-articular lesions, and treatment of traumatic intra-articular pathologies. The aim of this systematic review and meta-analysis was to provide evidence on whether ARIF is an alternative treatment protocol for ankle fractures.

Methods

The PubMed, Embase, and Cochrane Library databases were searched independently by two investigators from the inception dates to October 9, 2022, for comparative studies. The risk-of-bias tool of the Cochrane Collaboration for Randomized Controlled Trials and the methodological index for non-randomized studies (MINORS) were used for assessing the methodological quality. Outcomes were evaluated in terms of the Olerud–Molander Ankle Score (OMAS), American Orthopaedic Foot and Ankle Society (AOFAS) Ankle–Hindfoot Scale, post-operative complications, arthroscopic findings, Visual Analogue Scale (VAS) score, and operation time. Cochrane Review Manager Software 5.4 was used to perform the statistical analysis.

Results

A total of 10 trials involving 755 patients were included in this meta-analysis. The results revealed that ARIF for ankle fractures was superior regarding functional outcomes and VAS scores when compared with open reduction and internal fixation (ORIF). No significant difference was noted in the post-operative complication rate and the operation time between the ARIF and ORIF groups. A high incidence of chondral or osteochondral lesions (OCLs), ligamentous injuries, and loose bodies with ankle fractures was found by ankle arthroscopy.

Conclusions

ARIF for ankle fractures might be beneficial to offer superior functional outcomes and VAS score than ORIF. Orthopedic surgeons should take a high incidence of OCLs and ligamentous injuries into consideration for the treatment of acute ankle fractures. We believe that with the increase in surgical experience, the occurrence of post-operative complications and the extension of operation time will no longer be a potential concern for surgeons.
Total pancreatectomy with islet autotransplantation reduces opioid use and improves nutritional support in children with debilitating pancreatitis
Authors: Heinzman Christie , Hornung Lindsey , Lin Tom K. , Lowe Colleen M. O. , Vitale David S. , Abu-El-Haija Maisam , Nathan Jaimie D.
Published: August 2023

Background

Chronic pancreatitis (CP) can result in opioid dependence and nutritional challenges in children. Total pancreatectomy with islet autotransplantation (TPIAT) is a viable surgical option in appropriately selected patients. We examined differences between children who met criteria for TPIAT versus those who did not and continued with non-operative management.

Methods

Retrospective observational cohort study of patients evaluated for TPIAT between August 2014 and July 2020 was performed. Cohort-based analyses between TPIAT and non-TPIAT groups were performed.

Results

Analyses included 121 patients, 69 of whom underwent TPIAT. Demographics, genetic risk factors, and anatomic variants did not differ between groups. TPIAT patients were more likely to have CP (88% vs 71%; p = 0.02), had higher median number of endoscopic retrograde cholangiopancreatography procedures (2.0 vs 1.0; p = 0.0001), and had higher likelihood of opioid use (61% vs 42%; p = 0.04) and nutritional supplementation (23% vs 4%; p = 0.004), compared to non-TPIAT. At 6 months post-TPIAT, patients had lower use of any analgesic pain medications (39% vs 73%; p = 0.0002) and lower use of opioids (9% vs 39%; p = 0.0006), compared to non-TPIAT patients at 6 months after evaluation. At 6 months post-TPIAT, rate of exclusively oral nutrition increased from 77% to 86%, and total parenteral nutrition use decreased from 13% to 0% (p = 0.02).

Conclusions

In children referred for TPIAT evaluation, there is greater burden of disease in those selected for operation, compared to patients who do not undergo operation. TPIAT achieves lower analgesic pain medication use compared to continuation with non-TPIAT management and achieves freedom from nutritional supplementation.

Level of evidence: Retrospective comparative study, Level III.
Association of cyclooxygenase-2 expression with endoplasmic reticulum stress and autophagy in triple-negative breast cancer
Authors: Lee Haechan , Jung SungWook , Gong Gyungyub , Lim Bora , Lee Hee Jin
Published: August 2023

Cyclooxygenase-2 plays a role in oncogenesis and its overexpression is associated with triple-negative breast cancer. However, the mechanisms whereby cyclooxygenase-2 contribute to breast cancer are complex and not well understood. Cyclooxygenase-2 overexpression causes hypoxia, oxidative stress, and endoplasmic reticulum stress. The aim of this study is to investigate the correlations among cyclooxygenase-2 expression, endoplasmic reticulum stress-associated molecules, and autophagy-associated molecules in triple-negative breast cancer. Surgical specimens from two cohorts of triple-negative breast cancer patients without neoadjuvant systemic therapy were analyzed: cohorts 1 and 2 consisted of 218 cases from 2004 to 2006 and 221 cases from 2007 to 2009, respectively. Specimens were evaluated by immunohistochemical examination of cyclooxygenase-2, endoplasmic reticulum stress markers, and autophagy markers expression using tissue microarrays. Cyclooxygenase-2 was overexpressed in 29.8% and 23.9% of cases in cohorts 1 and 2, respectively; and it was positively correlated with two out of three endoplasmic reticulum stress-associated molecules (XBP1, p = 0.025 and p = 0.003 in cohort 1 and cohort 2, respectively; PERK, p < 0.001 in both cohorts). Cyclooxygenase-2 was also positively correlated with two out of three autophagy markers (p62, p = 0.002 and p = 0.003 in cohort 1 and cohort 2, respectively; beclin1, p < 0.001 in both cohorts). Although cyclooxygenase-2 was not an independent prognostic factor for distant metastasis free survival and overall survival, its expression was associated with the expression of endoplasmic reticulum stress and autophagy molecules in triple-negative breast cancer.
Morphometric characterization and temporal temperature measurements during hepatic microwave ablation in swine
Authors: Varble Nicole A. , Bakhutashvili Ivane , Reed Sheridan L. , Delgado Jose , Tokoutsi Zoi , Frackowiak Bruno , Baragona Marco , Karanian John W. , Wood Bradford J. , Pritchard William F.
Published: August 2023

Purpose

Heat-induced destruction of cancer cells via microwave ablation (MWA) is emerging as a viable treatment of primary and metastatic liver cancer. Prediction of the impacted zone where cell death occurs, especially in the presence of vasculature, is challenging but may be achieved via biophysical modeling. To advance and characterize thermal MWA for focal cancer treatment, an in vivo method and experimental dataset were created for assessment of biophysical models designed to dynamically predict ablation zone parameters, given the delivery device, power, location, and proximity to vessels.

Materials and methods

MWA zone size, shape, and temperature were characterized and monitored in the absence of perfusion in ex vivo liver and a tissue-mimicking thermochromic phantom (TMTCP) at two power settings. Temperature was monitored over time using implanted thermocouples with their locations defined by CT. TMTCPs were used to identify the location of the ablation zone relative to the probe. In 6 swine, contrast-enhanced CTs were additionally acquired to visualize vasculature and absence of perfusion along with corresponding post-mortem gross pathology.

Results

Bench studies demonstrated average ablation zone sizes of 4.13±1.56cm2 and 8.51±3.92cm2, solidity of 0.96±0.06 and 0.99±0.01, ablations centered 3.75cm and 3.5cm proximal to the probe tip, and temperatures of 50 ºC at 14.5±13.4s and 2.5±2.1s for 40W and 90W ablations, respectively. In vivo imaging showed average volumes of 9.8±4.8cm3 and 33.2±28.4cm3 and 3D solidity of 0.87±0.02 and 0.75±0.15, and gross pathology showed a hemorrhagic halo area of 3.1±1.2cm2 and 9.1±3.0cm2 for 40W and 90W ablations, respectfully. Temperatures reached 50ºC at 19.5±9.2s and 13.0±8.3s for 40W and 90W ablations, respectively.

Conclusion

MWA results are challenging to predict and are more variable than manufacturer-provided and bench predictions due to vascular stasis, heat-induced tissue changes, and probe operating conditions. Accurate prediction of MWA zones and temperature in vivo requires comprehensive thermal validation sets.
Norms in action? On the channels through which Poland’s historical partitions may still contribute to divergent educational achievements in the country’s regions
Authors: Herbst Mikołaj
Published: August 2023

The goal of this paper is to better understand the importance of a long-erased border between two empires that once partitioned Poland and its ongoing effect on regional educational achievement. Previous research has indicated that inherited norms towards education may explain the observed gap in the achievement of students in the former Austrian and Russian partitions of Poland. Findings suggest that although a gap in favour of the former Austrian partition does indeed exist in four school subjects, there is no convincing evidence of the causal effect of historically rooted norms on the achievements of today’s students. If such an effect exists, it is probably indirect and works through the accumulated educational attainments of adult generations and quality of instruction rather than by directly influencing the attitudes of today’s students.
Chinese yuan interest rate swap yields
Authors: Akram Tanweer , Mamun Khawaja
Published: August 2023

This paper models the dynamics of Chinese yuan–denominated long-term interest rate swap yields. It shows that the short-term interest rate exerts a decisive influence on the long-term swap yield after controlling for various macrofinancial variables, such as core inflation, the growth of industrial production, the percent change in the equity price index, and the percentage change in the Chinese yuan exchange rate. The autoregressive distributed lag approach is applied to model the dynamics of the long-term swap yield. The findings reinforce and extend John Maynard Keynes’s conjecture that in advanced countries, as well as emerging market economies such as China, the central bank’s actions have a decisive role in setting the long-term interest rate on government bonds and over-the-counter financial instruments, such as swaps.
In vivo Polycystin-1 interactome using a novel Pkd1 knock-in mouse model
Authors: Lin Cheng-Chao , Menezes Luis F. , Qiu Jiahe , Pearson Elisabeth , Zhou Fang , Ishimoto Yu , Anderson D. Eric , Germino Gregory G.
Published: August 2023

PKD1is the most commonly mutated gene causing autosomal dominant polycystic kidney disease (ADPKD). It encodes Polycystin-1 (PC1), a putative membrane protein that undergoes a set of incompletely characterized post-transcriptional cleavage steps and has been reported to localize in multiple subcellular locations, including the primary cilium and mitochondria. However, direct visualization of PC1 and detailed characterization of its binding partners remain challenging. We now report a new mouse model with HA epitopes and eGFP knocked-in frame into the endogenous mousePkd1gene by CRISPR/Cas9. Using this model, we sought to visualize endogenous PC1-eGFP and performed affinity-purification mass spectrometry (AP-MS) and network analyses. We show that the modifiedPkd1allele is fully functional but the eGFP-tagged protein cannot be detected without signal amplification by secondary antibodies. Using nanobody-coupled beads and large quantities of tissue, AP-MS identified anin vivoPC1 interactome, which is enriched for mitochondrial proteins and components of metabolic pathways. These studies suggest this mouse model and interactome data will be useful to understand PC1 function, but that new methods and brighter tags will be required to track endogenous PC1.
Spaces of reconnection
Authors: Afolabi Taiwo , Shercliff Emma , Speight Elaine
Published: August 2023

In this editorial we pick up again the theme of ‘connection’. Over the last year, the disconnections from our creative communities provoked by the COVID-19 pandemic have loomed large on our horizon: how do we set about reconnecting? Articles presented in this volume examine these disconnections and explore the ways artists, designers, curators, photographers, gamers, dancers and others draw upon expanded approaches to creativity, initiating imaginative and resourceful ways to reconnect with their audiences and communities.
Taking a seat: Curating spaces of (un)learning
Authors: Halliday Amy , Butcher Clare , Metaferia Helina
Published: August 2023

Through an edited conversation, curator-educators Amy Halliday and Clare Butcher engage with artist-scholar Helina Metaferia whose work acts as a catalyst for further discussion around various inheritances, questions and approaches shaping curatorial and artistic methodologies in relation to spaces of ‘knowledge production’ and practices of study in a more expanded sense. Be it within university gallery settings, biennial programming or in research-based collaborations, the pedagogically grounded curatorial and artistic models Halliday, Butcher and Metaferia activate and learn with often sit uncomfortably between hierarchies of visibility and value, process and product, learning and unlearning, education and representation. By sharing a number of exhibition, programming and artist-led case studies – including Halliday’s work with Helina Metaferia – in the development of the Against a Sharp White Background () exhibition and Butcher’s collaborations shaping recent programming and learning projects, informed by the work of artists such as Annette Kraus (on the ‘hidden curriculum’) and the collective work of Gesturing Towards Decolonial Futures – the authors reflect on the generative lines of inquiry prompted by socially engaged, context-responsive artists’ practices working in these uncomfortable intersections. Halliday, Butcher and Metaferia also consider their own embeddedness in colonial, institutionalized art histories and the curatorial ethics that might transform these canons. Through the conversation, various questions emerge: what are the shared and uncommon vocabularies and positionalities that emerge through critical and collaborative curating and creative practice across colonial histories and presents? How are roles and accountabilities determined where participating stakeholders – such as artists, students, faculty or community members – are both authors and audiences? Who is acknowledged as expert or knowledge keeper in such conflicting value systems? Which voices and genealogies of practice inform the curricula we collectively create in pedagogical processes? Can we practise care in careless systems that are temporally bound and often output-oriented?
Inclusive curatorial strategies: A case study of an interdisciplinary conference and festival: Communities and Communication
Authors: Coleclough Sharon , Lulkowska Agata , Steventon Stephanie
Published: August 2023

The article explores the curatorial strategies involved in the completion of an interdisciplinary conference. Considering factors of control, space, opportunity and most importantly generating a safe space to experiment and participate in an academic conference to those generally seen as outside the academy. With this in mind the work explores the ways in which the event became a testing ground and developmental jumping off point to identify the ways in which support can be offered to a widened participant pool.
Different Models, One Goal: Doing Theatre, Democracy and Social Justice: A Festschrift in Honour of Prof. Tor Joe Iorapuu, Adediran Kayode Ademiju-Bepo, Umaru Hussaini Tsaku, Tijime Justin Awuawuer, Shadrack Teryila Ukuma and Ben Alfred Abugh (eds) (2022)
Authors: Dugga Victor S. , Onwe Mark O.
Published: August 2023

Review of: Different Models, One Goal: Doing Theatre, Democracy and Social Justice: A Festschrift in Honour of Prof. Tor Joe Iorapuu, Adediran Kayode Ademiju-Bepo, Umaru Hussaini Tsaku, Tijime Justin Awuawuer, Shadrack Teryila Ukuma and Ben Alfred Abugh (eds) (2022)

Jos: DynastyGold Global Impact Communications, 789 pp.,

ISBN 978-978-55547-6-2, h/bk, $21.67
Five-axis parallel mechanism system (PMS) CNC partial link control system based on modified inverse kinematic of 6-DOF UPS parallel manipulator
Authors: Ramadhan Nur Jamiludin , Indrawanto Indrawanto , Nguyen Hoe Dinh
Published: August 2023

This paper presents a control system algorithm for a five-axis parallel mechanism system (PMS) CNC milling machine based on a 6-DOF Stewart platform parallel manipulator with a universal-prismatic-spherical (UPS) configuration. The control system reads the G-Code commands as standard CNC machine language, then extract data points and interpolates them to generate the robot trajectory patterns as motion references. Then, the control system uses the modified inverse kinematic equation to determine the length of each link to move the end effector to track the trajectory patterns from the previous G-code extraction process. The inverse kinematic equation is modified especially for the five-axis PMS CNC milling machine by including machine-offset and tools-offset parameters so it will be easier for the control system to implement the kinematic equation. As expected, the system simulation results successfully followed the G-Code program moving commands. The average error of the length control system is 0,1 mm, while the average error of the length change rate control system is 1,8 mm/s. The maximum error is 26.9 mm was caused by the system's inability to follow the motion profile in transient. It can be concluded that 6-DOF Stewart platform parallel structures,which provide better performance than serial structures, can be implemented as a new concept for the motion mechanism of five-axis CNC milling machines. The five-axis PMS CNC milling machine also promises better performance than conventional five-axis gantry structures CNC.
LSTM-based forecasting on electric vehicles battery swapping demand: Addressing infrastructure challenge in Indonesia
Authors: Romdlony Muhammad Zakiyullah , Khayr Rashad Abul , Muharam Aam , Priandana Eka Rakhman , Sasmono Sudarmono , Rosa Muhammad Ridho , Purnama Irwan , Amin Amin , Fachri Ridlho Khoirul
Published: August 2023

This article aims to design a model for forecasting the number of vehicles arriving at the battery swap station (BSS). In our case, we study the relevance of the proposed approach given the rapid increase in electric vehicle users in Indonesia. Due to the vehicle electrification program from the government of Indonesia and the lack of supporting infrastructure, forecasting battery swap demands is very important for charging schedules. Forecasting the number of vehicles is done using machine learning with the long short-term memory (LSTM) method. The method is used to predict sequential data because of its ability to review previous data in addition to the current input. The result of the forecasting using the LSTM method yields a prediction score using the root-mean-square error (RMSE) of 2.3079 x 10-6 . The forecasted data can be combined with the battery charging model to acquire predicted hourly battery availability that can be processed further for optimization and scheduling.
The Road to the Clinic: BYON4228
Authors:   Byondis, Timmers Marco , Van den Berg Timo K. ,   Byondis
Published: August 2023

The latest Byondis investigative therapy that will soon enter Phase I or First-in-Human (FiH) study is also the company’s first immuno-oncology (IO) therapeutic, BYON4228. How and why it got there is the stuff that researchers’ dreams are made of. BYON4228 is a monoclonal antibody (mAb). A mAb is – per the US National Cancer Institute (NCI), “a type of protein that is made in the laboratory and can bind to certain targets in the body, such as antigens on the surface of cancer cells.” Monoclonal antibodies can be used to diagnose and treat many diseases, such as cancers. They can be used alone or to carry drugs, toxins, or radioactive substances directly to cancer cells
“If I Can See It, I Can Kill It”
Authors:   Hackensack Meridian Health, Pecora Andrew
Published: August 2023

If one could use the word “elegant” to describe anticancer therapy, that moniker would apply to messenger RNA (mRNA) vaccines. The elegance of mRNA vaccines derives from their apparent ability to overcome one of the major limitations of conventional cancer vaccines: HLA restriction. Most cancer vaccines are composed of glycoproteins that only fit inside the antigen-presenting pockets in human leucocyte antigen (HLA) molecules – our cellular “home address” – a certain percentage of the time. Consequently, anyone treated with a protein-based vaccine is fortunate to achieve an immune response.
Prescribed Fire Use Among Black Landowners in the Red Hills Region, USA
Authors: Perkins La' Portia J. , Coates T. Adam , Hiers J. Kevin , Fowler Cynthia T. , Bigelow Seth W.
Published: August 2023

The Red Hills Region of southern Alabama, northern Florida, and southwestern Georgia is one of the most prominent areas in the United States for conducting prescribed fire research and is the birthplace of fire ecology. The culture of prescribed burning in the Red Hills has been influenced by multiple ethnic groups, including the Seminole and Creek nations, Black landowners, and White researchers. Given the distinctive reliance of the region on prescribed fire, it is noteworthy that the combined issues of Black land loss, underrepresentation, and incentives for using prescribed fire on private lands in the southeastern United States have generated questions about diversity and inclusion in landowner outreach. To increase understanding about Black landowner historic and current use of prescribed fire for land management in the Red Hills Region, formal and informal interviews were conducted from May through August 2019 with 21 Black landowners and tenants to document the perspectives and thoughts of Black landowners and tenants of southern Alabama, northern Florida, and southwestern Georgia. The results of this research show that Black landowners, tenants, and fire experts, have been, and continue to be, influential in the development and sustainment of fire traditions in the Red Hills and in the resilience of the longleaf pine ecosystem.
Multidisciplinary Diagnosis and Management of a Rare Dysplastic Adenoma Involving a Pancreas Transplant
Authors: Kayaalp Cemil , Are Vijay S. , Kandaswamy Raja , Amateau Stuart K.
Published: August 2023

A 52-year-old man with a history of type 1 diabetes mellitus with diabetic nephropathy who underwent simultaneous pancreas–kidney transplant over a decade ago presented with small bowel obstruction and was found by enteroscopy to have a carpeted lesion encompassing the small bowel anastomosis in the region of the donor pancreas. As endoscopic mucosal resection was impracticable because of technical limitations, the patient was referred to transplant surgical team for surgical exploration and ultimately required organ resection. This represents a unique presentation of an ampullary adenoma with high-grade dysplasia requiring device-assisted enteroscopy requiring multidisciplinary management.
Timely Endoscopic Recognition of Aortoesophageal Fistula With Successful Treatment
Authors: Brotherton Tim , Numan Laith , Al-Kaade Samer
Published: August 2023

Aortoesophageal fistula (AEF) is a devastating cause of upper gastrointestinal bleeding that occurs because of pathologic communication of the esophagus with the aorta. Bleeding AEF has high mortality even with appropriate interventions. In this study, we present a case of a 52-year-old man who presented with hematemesis, which was found to be due to an actively bleeding AEF. Prompt identification of the fistula led to multiple endoscopic interventions that stabilized the patient and allowed him to undergo emergent endovascular aortic stent placement with successful bleeding control.
Higher education as ‘strategic power’? An assessment of China-Africa higher education partnerships and collaborations
Authors:   University of Liverpool, Hodzi Obert , Amoah Padmore ,   Lingnan University
Published: August 2023

China is internationalizing its higher education sector – setting up several bilateral and multilateral partnerships between public and private institutions across the globe. However, as the “West” is disentangling itself from partnerships with Chinese institutions of higher education and the Confucius Institutes (CIs), African countries seem to be turning to Beijing. As a result, China overtook France to become the most preferred destination for African students. But, is higher education Beijing’s new strategy to enhance its global status? What is the effect of the shift toward Chinese higher education on Africa’s migration trends, and what is the agency of actors in Africa? Focusing on these questions, and premised on the concepts of student mobility, South–South Cooperation (SSC), and people-to-people exchange to explain the novelty and exceptionality of the partnerships, this paper explores the typology, nature, and processes involved in these partnerships and collaborations.
Como trabalhar em relação cêntrica com alinhadores
Authors: Janson Marcos
Published: August 2023

INTRODUÇÃO: Quando se faz o tratamento ortodôntico, utiliza-se a relação cêntrica (RC) para diagnosticar e tratar o paciente. Ao utilizar aparelhos fixos, essa conduta pode ser realizada pela manipulação do paciente na consulta inicial e nas demais. Com o advento dos alinhadores ortodônticos, a manipulação de diagnóstico pode ser realizada na consulta inicial; no entanto, como os alinhadores já vêm prontos, é necessário um escaneamento em RC para que a direção de tratamento seja a desejada. OBJETIVO: Esse artigo tem o objetivo de demonstrar como se faz esse procedimento e a diferença que ele promove nas decisões dos profissionais.
Tratamento da Classe II subdivisão utilizando miniparafusos extra-alveolares na crista infrazigomática, associados a aparelhos autoligáveis com prescrição individualizada: relato de caso
Authors: Villela Henrique Mascarenhas
Published: August 2023

INTRODUÇÃO: A Classe II subdivisão é um tipo de má oclusão assimétrica que pode ser decorrente de um problema esquelético ou dentoalveolar. Para corrigi-la da forma mais adequada, é imprescindível fazer o diagnóstico diferencial, escolhendo a melhor estratégia de tratamento. As más oclusões de Classe II subdivisão de natureza dentoalveolar podem ser tratadas com mesialização de dentes inferiores e/ou distalização de dentes superiores. Os miniparafusos ortodônticos extra-alveolares fornecem uma ancoragem esquelética eficiente para efetuar correções sagitais dentoalveolares, tanto com a mesialização dos dentes inferiores quanto com a distalização dos superiores, anulando os efeitos colaterais indesejados, por meio de procedimentos minimamente invasivos. Esses dispositivos eliminam, ainda, o inconveniente da inserção dos miniparafusos entre as raízes dentárias, aumentando a eficiência do tratamento nas correções sagitais e simplificando a mecânica ortodôntica. A associação desse recurso de ancoragem com os aparelhos autoligáveis passivos com prescrições individualizadas reduz a necessidade de dobras nos arcos. OBJETIVO: Relatar um caso clínico no qual foram utilizados parafusos extra-alveolares na crista infrazigomática como recurso de ancoragem, associados a aparelhos autoligáveis passivos, para corrigir uma Classe II subdivisão direita. CONCLUSÃO: A associação dos miniparafusos extra-alveolares com o sistema de aparelhos autoligáveis passivos com prescrições individualizadas se mostrou eficiente na correção da má oclusão de Classe II subdivisão, efetuando uma maior distalização do lado superior direito, posicionando os primeiros molares simetricamente na arcada e reduzindo a inclinação dos incisivos superiores.
Entrevista com Oyku Dalci
Authors: Dalci Oyku , Tuba Altug Ayşe , Faber Jorge , Littlewood Simon J
Published: August 2023

Em 2015, fui convidado para avaliar os alunos de pós-graduação em Ortodontia na Universidade de Sydney, ocasião em que conheci a professora sênior Dra. Oyku Dalci. A Dra. Dalci formou-se na Turquia, mas desde 2009 trabalha no Departamento de Ortodontia e Odontopediatria da Faculdade de Odontologia de Sydney, Universidade de Sydney, Austrália. Esse departamento é liderado pelo carismático e bem-sucedido professor Ali Darendeliler, que foi o primeiro a me dizer o quão importante a Dra. Dalci é para o Departamento de Ortodontia — o que eu pude testemunhar em primeira mão enquanto trabalhava ao seu lado em Sydney.
Elásticos de Classe III: os vetores verticais podem ser nossos aliados
Authors: Meireles José Kleber Soares de
Published: August 2023

INTRODUÇÃO: Elásticos de Classe III são excelentes ferramentas nos tratamentos compensatórios desse tipo de má oclusão. Porém, somam-se às forças horizontais, que promovem a correção sagital do problema, outros vetores que, em certos casos, podem levar a efeitos indesejáveis. Um desses efeitos é a extrusão dos dentes posteriores superiores e anteriores inferiores. Um ponto importante nesses casos é entender quando esses movimentos extrusivos são realmente negativos e quando eles podem ser desejáveis. OBJETIVO: O objetivo desse artigo é descrever os protocolos de tratamento com elásticos de Classe III e como os efeitos verticais desses elásticos podem, quando bem indicados, nos auxiliar na obtenção de uma mecânica mais rápida, segura e assertiva.
Sobre a exposição Premium Connect, de Tabita Rezaire: a reativação do potencial terapêutico e político da magia
Authors: Castelo Branco Sara
Published: August 2023

Envolvendo referências do misticismo, da ciência, da história africana e das culturas pós-Internet, este texto parte da exposição Premium Connect, de Tabita Rezaire, apresentada no Batalha Centro de Cinema, no Porto, para abordar as conexões entre o espiritual e o digital, evocando problemáticas que envolvem a conectividade global do digital.