bluuebunny/crossref_metadata_2025 huggingface dataset:
Whether Urbanization Has Intensified the Spread of Infectious Diseases—Renewed Question by the COVID-19 Pandemic
Authors: Yu Dongsheng , Li Xiaoping , Yu Juanjuan , Shi Xunpeng , Liu Pei , Tian Pu
Published: November 2021

The outbreak of the COVID-19 epidemic has triggered adiscussion of the relationship between urbanization and the spread of infectious diseases. Namely, whether urbanization will exacerbate the spread of infectious diseases. Based on 31 provincial data from 2002 to 2018 in China, the impact of urbanization on the spread of infectious diseases from the dimensions of “population” and “land” is analyzed in this paper by using the GMM (generalized method of moments) model. The empirical study shows that the population increase brought by urbanization does not aggravate the spread of infectious diseases. On the contrary, urban education, employment and entrepreneurship, housing, medical and health care, and other basic public services brought by population urbanization can help reduce the risk of the spread of infectious diseases. The increasing density of buildings caused by land urbanization increases the risk of the spread of infectious diseases. Moreover, the impact of urbanization on the spread of infectious diseases has regional heterogeneity. Therefore, the prevention and control of disease play a crucial role.
Perspective Commentary: The Implementation of Welfare Policies Are Not Held to the Same Ethical Standards as Research: Raising Intergenerational Health Inequality Concerns
Authors: Wickham Sophie , Fancourt Daisy
Published: November 2021

Over the last 12 years the United Kingdom (UK) has seen the introduction of an austerity programme—a fiscal policy—with the primary goal to reduce the government's budget deficit and the role of the welfare system. Between 2010 and 2015 there was an estimated reduction of £14.5 billion in spending, attributable to decreasing the value of benefits and restricting entitlement to benefit claimants. By 2020, there had been an estimated unprecedented £27 billion less spent on welfare compared with spending in 2010. Whilst fiscally-successful at reducing spending, some implemented welfare policies have had direct consequences for people's health, increasing inequalities which have been heavily criticized. Moreover, there is growing concern that this has an intergenerational effect. In this paper, we describe the ethical principles in human research, how these have been considered in public health policy, and the existing evidence of the direct and intergenerational health and welfare consequences of some recent, nationally-implemented welfare policies. We argue that ethical principles, specifically the ethical principle of safety that is applied in all research, should be applied to all public welfare policies to stop the rising inequalities in health we are seeing across generations. We highlight that initial changes implemented to welfare policies as a response to COVID-19 demonstrate that there can be a political and societal perceived value in going further to support individuals and their families during times of adversity, and consider the ethical implications of this.
Point-of-Care Diagnostic Tools for Surveillance of SARS-CoV-2 Infections
Authors: Sakthivel Dhanasekaran , Delgado-Diaz David , McArthur Laura , Hopper William , Richards Jack S. , Narh Charles A.
Published: November 2021

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a recently emerged and highly contagious virus that causes coronavirus disease 2019 (COVID-19). As of August 24, 2021, there were more than 212 million confirmed COVID-19 cases and nearly 4.4 million deaths reported globally. Early diagnosis and isolation of infected individuals remains one of the most effective public health interventions to control SARS-CoV-2 spread and for effective clinical management of COVID-19 cases. Currently, SARS-CoV-2 infection is diagnosed presumptively based on clinical symptoms and confirmed by detecting the viral RNA in respiratory samples using reverse transcription polymerase chain reaction (RT-PCR). Standard RT-PCR protocols are time consuming, expensive, and technically demanding, which makes them a poor choice for large scale and point-of-care screening in resource-poor settings. Recently developed isothermal nucleic acid amplification tests (iNAAT), antigen and/or serological tests are cost-effective to scale COVID-19 testing at the point-of-care (PoC) and for surveillance activities. This review discusses the development of rapid PoC molecular tools for the detection and surveillance of SARS-CoV-2 infections.
Super-Small Predators in Soils: Who Are They and What Do They Do?
Authors: Geisen Stefan
Published: November 2021

There are millions of species living in soils. Most of this biodiversity is made up of bacteria and fungi, tiny organisms that make up what is called the soil microbiome. The size and composition of the soil microbiome is mainly controlled by two groups of predators: protists and nematodes. Protists are tiny single-celled organisms, while nematodes are tiny worms and the most numerous animals on Earth. Protists and nematodes together weight more than all the other animals on Earth! Protists and nematodes keep the soil microbiome in balance, which helps plants to grow and keeps soils functioning properly. Without protist and nematode soil predators, the functions and services provided by soils would change so much that it could even affect the Earth’s climate. So, let us not forget the importance of these tiny soil organisms!
Cryostimulation for Post-exercise Recovery in Athletes: A Consensus and Position Paper
Authors: Bouzigon Romain , Dupuy Olivier , Tiemessen Ivo , De Nardi Massimo , Bernard Jean-Pierre , Mihailovic Thibaud , Theurot Dimitri , Miller Elzbieta Dorota , Lombardi Giovanni , Dugué Benoit Michel
Published: November 2021

Recovery after exercise is a crucial key in preventing muscle injuries and in speeding up the processes to return to homeostasis level. There are several ways of developing a recovery strategy with the use of different kinds of traditional and up-to-date techniques. The use of cold has traditionally been used after physical exercise for recovery purposes. In recent years, the use of whole-body cryotherapy/cryostimulation (WBC; an extreme cold stimulation lasting 1–4 min and given in a cold room at a temperature comprised from −60 to −195°C) has been tremendously increased for such purposes. However, there are controversies about the benefits that the use of this technique may provide. Therefore, the main objectives of this paper are to describe what is whole body cryotherapy/cryostimulation, review and debate the benefits that its use may provide, present practical considerations and applications, and emphasize the need of customization depending on the context, the purpose, and the subject's characteristics. This review is written by international experts from the working group on WBC from the International Institute of Refrigeration.
Pure Laparoscopic vs. Open Right Hepatectomy in Living Liver Donors: Bench-Surgery Time
Authors: Hong Kwangpyo , Hong Suk Kyun , Han Eui Soo , Suh Sanggyun , Hong Su young , Lee Jeong-Moo , Choi YoungRok , Yi Nam-Joon , Lee Kwang-Woong , Suh Kyung-Suk
Published: November 2021

Background: Recently, there have been several reports on pure laparoscopic donor right hepatectomy (PLDRH), but the effect of pure laparoscopy on bench surgery has not been evaluated. This study aimed to compare bench-surgery time between PLDRH and conventional donor right hepatectomy (CDRH).

Methods: We retrospectively reviewed the medical records of 758 live liver donors between January 2012 and December 2019. We divided the patients into two groups: between January 2012 and September 2015, when we exclusively performed CDRH, and between March 2016 and December 2019, when PLDRH was standardized. We excluded all other types of graft donor hepatectomy, laparoscopic assisted donor hepatectomy, and cases with no recorded data.

Results: In total, 267 donors were included in the PLDRH group and were compared with 247 donors in the CDRH group. Similar proportions of graft vascular variations were observed between the two groups. The mean bench-surgery time was longer in the PLDRH group than in the CDRH group (49.3 ± 19.9 vs. 39.5 ± 17.5 min; P < 0.001).

Conclusion: The bench-surgery time was longer in the PLDRH group than the CDRH group, regardless of whether the vascular network was reconstructed. Expertise in bench-surgery as well as donor surgery and recipient surgery is mandatory for PLDRH to be safe and feasible.
Evaluation of Pre-operative Biopsy, Surgical Procedures and Oncologic Outcomes in Upper Tract Urothelial Carcinoma (UTUC)
Authors: Koll Florestan J. , Meisenzahl Eva , Haller Bernhard , Maisch Philipp , Kirchhoff Florian , Horn Thomas , Gschwend Jürgen E. , Schmid Sebastian C.
Published: November 2021

Purpose: Discordance between pre-operative biopsy and final pathology for Upper Tract Urothelial Carcinoma (UTUC) is high and optimal management remains controversial. The aim of this study is to evaluate the accuracy of pre-operative biopsy, to identify prognostic factors and to evaluate the effect of adjuvant chemotherapy on survival and oncologic outcome in UTUC.

Methods: We analyzed records of patients receiving surgical treatment for UTUC. Pathology of pre-operative biopsy was compared to surgical specimen. We used Kaplan-Meier method to estimate survival probabilities and Cox's proportional hazards models to estimate the association between covariates and event times. Primary endpoint was overall survival (OS). A matched-pair analysis was performed to evaluate the effect of adjuvant chemotherapy.

Results: 151 patients underwent surgical treatment (28% open, 36% laparoscopic, 17% robotic radical nephroureterectomy; 14% segmental ureteral resections and 5% palliative nephrectomy) for UTUC and were included in the analysis. Upstaging from <pT1 in endoscopic biopsy to ≥pT1 in final pathology occurred in 61% of patients and upgrading from low-grade to high-grade occurred in 30% of patients. Five-year OS was 59.5%. In the univariate Cox-regression model pathological stage, grade, lymphovascular invasion and positive surgical margins were associated with OS. Matched pair analysis for stage (<pT3; ≥pT3; pN+) and age revealed a significant survival benefit for adjuvant chemotherapy (HR 0.40, 0.14–0.77, p < 0.018) in this cohort.

Conclusion: UTUC is often underestimated in pre-operative biopsy, and it is associated with significant mortality. Pathological stage and grade, lymphovascular invasion and lymph node metastases are predictors of oncologic outcome and survival.
Molecular Cloning of Alternative Splicing Variants of the Porcine PML Gene and Its Expression Patterns During Japanese Encephalitis Virus Infection
Authors: Zhu Jingjing , Chen Zhenyu , Dai Zhenglie , Zhou Xiaolong , Wang Han , Li Xiangchen , Zhao Ayong , Yang Songbai
Published: November 2021

Promyelocytic leukemia (PML) protein is a crucial component of PML-nuclear bodies (PML-NBs). PML and PML-NBs are involved in the regulation of various cellular functions, including the antiviral immune response. The human PML gene can generate several different isoforms through alternative splicing. However, little is known about the porcine PML alternative splicing isoforms and their expression profiles during Japanese encephalitis virus (JEV) infection. In the present study, we cloned seven mature transcripts of porcine PML, all of which contained the same N-terminal sequence but differed in the C-terminal sequences due to alternative splicing. These seven transcripts encoded five proteins all of which had the RBCC motif and sumoylation sites. Amino acid sequence homology analysis showed that porcine PML-1 had relatively high levels of identity with human, cattle, and goat homologs (76.21, 77.17, and 77.05%, respectively), and low identity with the mouse homolog (61.78%). Immunofluorescence analysis showed that the typical PML-NBs could be observed after overexpression of the five PML isoforms in PK15 cells. Quantitative reverse transcription PCR (RT-qPCR) analysis showed significant upregulation of PML isoforms and PML-NB-associated genes (Daxx and SP100) at 36 and 48 h post-infection (hpi). Western blotting analysis indicated that the PML isoforms were upregulated during the late stage of infection. Moreover, the number of PML-NBs was increased after JEV infection. These results suggest that porcine PML isoforms may play essential roles in JEV infection.
An Integrated Perspective on Virulence-Associated Genes (VAGs), Antimicrobial Resistance (AMR), and Phylogenetic Clusters of Pathogenic and Non-pathogenic Avian Escherichia coli
Authors: Rezatofighi Seyede Elham , Najafifar Arash , Askari Badouei Mahdi , Peighambari Seyed Mostafa , Soltani Mohammad
Published: November 2021

Avian pathogenic Escherichia coli (APEC) is an important bacterial pathogen that causes avian colibacillosis and leads to huge economic losses in the poultry industry. Different virulence traits contribute to pathogenesis of APEC infections, and antimicrobial resistance (AMR) has also been an overwhelming issue in poultry worldwide. In the present study, we aimed to investigate and compare the presence of virulence-associated genes (VAGs), AMR, and phylogenetic group's distribution among APEC and avian fecal E. coli (AFEC) strains. E. coli from birds with colisepticemia and yolk sac infection (YSI) (APEC) plus E. coli strains from the feces of healthy birds (AFEC) were compared by the aforementioned traits. In addition, the clonal relatedness was compared using Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Although all strains were susceptible to fosfomycin, ceftriaxone, and cefixime, almost all strains (98%) were multi-drug resistant (MDR). All strains (except two) harbored at least three or more VAGs, and the virulence scores tended to be higher in pathogenic strains especially in the colisepticemic group. All phylogenetic groups were found in isolates from YSI, colisepticemia, and the feces of healthy birds; however, the frequency of phylogroups varied according to the source of the isolate. B1 and C phylogroups were statistically more likely to be found among APEC from YSI and colisepticemic E. coli groups, respectively, while phylogroup A was the most frequently occurring phylogroup among AFEC strains. Our findings also revealed that AMR and VAGs are not essentially co-evolved traits as in some instances AMR strains were more prevalent among AFEC. This reflects the divergent evolutionary pathways of resistance acquisition in pathogenic or non-pathogenic avian E. coli strains. Importantly, strains related to phylogenetic group C showed higher virulence score and AMR that requires further attention. To some extent, ERIC-PCR was able to group strains by isolation source, phylogroup, or virulence genes. Further integrated studies along with assessment of more detailed genotypic and phenotypic features could potentially lead to better understanding of virulence, resistance, and evolution of ExPEC.
A Multi-Curvature, Variable Stiffness Soft Gripper for Enhanced Grasping Operations
Authors: Song Eun Jeong , Lee Jung Soo , Moon Hyungpil , Choi Hyouk Ryeol , Koo Ja Choon
Published: November 2021

For soft grippers to be applied in atypical industrial environments, they must conform to an object’s exterior shape and momentarily change their stiffness. However, many of the existing grippers have limitations with respect to these functions: they grasp an object with only a single curvature and a fixed stiffness. Consequently, those constraints limit the stability of grasping and the applications. This paper introduces a new multicurvature, variable-stiffness soft gripper. Inspired by the human phalanx and combining the phalanx structure and particle jamming, this work guarantees the required grasping functions. Unlike the existing soft pneumatic grippers with one curvature and one stiffness, this work tries to divide the pressurized actuating region into three parts to generate multiple curvatures for a gripper finger, enabling the gripper to increase its degrees of freedom. Furthermore, to prevent stiffness loss at an unpressurized segment, this work combines divided actuation and the variable-stiffness capability, which guarantee successful grasping actions. In summary, this gripper generates multiple grasping curvatures with the proper stiffness, enhancing its dexterity. This work introduces the new soft gripper’s design, analytical modeling, and fabrication method and verifies the analytic model by comparing it with FEM simulations and experimental results.
Do Internet Skills Increase Farmers’ Willingness to Participate in Environmental Governance? Evidence from Rural China
Authors: He Qiang , Deng Xin , Li Chuan , Yan Zhongcheng , Qi Yanbin
Published: November 2021

Environmental pollution is threatening the sustainable development of rural areas. Increasing farmers’ willingness to participate in environmental governance (FWPEG) can effectively reduce this threat. Fortunately, the internet can speed up the process. However, it is unclear whether and to what extent the mastery of internet skills will increase FWPEG. This study uses data from 3503 farmers in 30 provinces in mainland China. It uses the TE and IVQTE models to correct selection bias and quantitatively assess the impact of mastery of internet skills on FWPEG. The results show: (1) mastering internet skills can significantly increase FWPEG, and after correcting the endogenous deviation, the marginal benefit of farmers mastering internet skills is 0.124; (2) in the 34–81% quantile range, internet skills show a declining development trend in FWPEG, which is in line with “the law of diminishing marginal utility”, and mastery of the impact of internet skills on FWPEG has “leaping” (33% → 34%)” and “sagging (81% → 82%)” characteristics; (3) compared to that of the east, internet skills in central and western regions have a more significant role in promoting FWPEG. In general, internet skills can effectively increase FWPEG, and the impact will be more pronounced in underdeveloped areas. The influence of internet skills on FWPEG will gradually weaken with the increase of FWPEG. The results of this research help to coordinate the relationship between government environmental governance and rural environmental autonomy and provide some new ideas for realizing global rural revitalization.
Factors Affecting Farm Succession and Occupational Choices of Nominated Farm Successors in Gilgit-Baltistan, Pakistan
Authors: Shahzad Muhammad Abid , Abubakr Syed , Fischer Christian
Published: November 2021

Mountain farming communities in Pakistan are exhibiting an increased rate of rural-to-urban migration and a rapid growth in the non-farm sector, which has threatened the sustenance of agricultural activity. This study examined the determinants of farm succession using a logit regression model and employed a multinomial logit regression model to study the factors influencing the future occupational choices of the potential farm successors. The study was based on quantitative survey data obtained from 421 farm managers and 155 potential farm successors and qualitative data from 12 key informants from two different districts in Gilgit-Baltistan. The survey results show that around 67% of the farmers had a potential successor. Farm succession was mainly explained by farmer characteristics (e.g., farmer age, gender and education), farm characteristics (e.g., farm size, specialization in horticulture, etc.) and agricultural income. Regarding the occupational choices, part-time farming (66%) was the most commonly reported choice. The results indicate that it was mainly farm successors’ personal characteristics (such as age, education and marital status) and agricultural income that led to the choices “undecided” and “exit”, whilst farm characteristics (e.g., farm size) and the main farm operators’ non-farm activity were significantly associated with the choice “part-time”. Policies aimed at improving the local income situation and investments in skill-building and infrastructure development can assist in farm sustenance.
Towards a Socio-Economic Model for Southwest Asian Cereal Domestication
Authors: Weide Alexander
Published: November 2021

Mechanisms of selection for domestication traits in cereals and other annual plants are commonly explained from agro-technological and genetic perspectives. Since archaeobotanical data showed that domestication processes were slow and protracted, research focused on genetic constraints and hypothetical ‘non-selective’ management regimes to explain the low selection rates. I argue that these factors only partially explain the observed patterns and develop a model that contextualises the archaeobotanical data in their socio-economic settings. I propose that developments towards individual storage by small household units and the gradual increase in storage capacities with the development of extended households represent key factors for establishing the conditions for selection, as these practices isolated individually managed and stored cereal subpopulations and gradually reduced the need to replenish grain stocks with grains from unmanaged populations. This genetic isolation resulted in stronger and more persistent selection rates and facilitated the genetic fixation of domestication traits on a population level. Moreover, individual storage facilities within buildings reflect gradual developments towards households as the social units that mobilised agricultural labour, which negotiated new sharing principles over cultivated resources and drove the intensification of cultivation practices. In this sense, selection rates and the slow domestication process can be understood as a function of limited food sharing networks and increased labour-inputs into early arable environments—socio-economic processes that also unfolded gradually over a protracted period of time.
Factors Affecting New Agricultural Business Entities’ Adoption of Sustainable Intensification Practices in China: Evidence from the Main Apple-Producing Areas in the Loess Plateau
Authors: Wang Zhao , Liu Jianhong , Li Tongsheng , Chao Jing , Gao Xupeng
Published: November 2021

The unsustainability of China’s agricultural production requires an urgent shift from traditional to more sustainable practices; however, the acceleration thereof remains challenging. New agricultural business entities (NABEs) lead agricultural modernization and strongly guide the application of innovative agricultural technologies and models. Thus, an understanding of the factors that influence NABEs’ adoption of sustainable intensification practices will promote their widespread adoption. We developed a model based on innovation diffusion theory and the technology–organization–environment framework, which can both distinguish the influencing factors at different adoption stages and identify the influencing factors of technology adoption from a multidimensional perspective. The results indicate that differences in regional agroecological endowments emerge as the most important influencing factor. Relative advantage, perceived barriers, and agricultural extension services have a significant effect on adoption intention and decision, but a smaller effect on intention. Management and risk response capacities have a significant positive effect on adoption decisions, but no effect on intention. Meanwhile, organizational size has no effect on adoption intention or decision. Adoption intention significantly positively influences, but only partially explains, adoption decisions. Our findings provide a basis for technology promoters to categorize potential adopters by technology adoption stage and provide targeted strategies to stimulate technology demand.
Comparative Efficiency of Mineral, Chelated and Nano Forms of Zinc and Iron for Improvement of Zinc and Iron in Chickpea (Cicer arietinum L.) through Biofortification
Authors: Dhaliwal Salwinder Singh , Sharma Vivek , Shukla Arvind Kumar , Verma Vibha , Behera Sanjib Kumar , Singh Prabhjot , Alotaibi Saqer S. , Gaber Ahmed , Hossain Akbar
Published: November 2021

Nanoparticles (NPs), due to their tailored properties, serve as potential sources of nutrients for the biofortification of edible grains. Chickpeas are a valued legume crop, widely consumed in developing countries. Thus, to improve the Zn and Fe content in chickpeas, a two-year study was conducted to examine the potential of the foliar application of mineral (0.5% Zn and Fe), chelated (0.3% Zn and Fe) and nanoforms (0.5% ZFN) of fertilizers to enhance Zn and Fe content in chickpea. The foliar application of 0.5% ZnO NPs + 0.5% Fe2O3 NPs (ZFN) at the pre-flowering stage showed the highest potential to increase grain yield, Zn and Fe content and their uptake as a single foliar application of nano-fertilizers showed comparable results to two foliar applications of mineral and chelated forms. The grain and straw yield (14.07 and 33.04 q ha−1, respectively) under ZFN treatment was significantly higher over the control (9.20 and 27.49 q ha−1, respectively). A similar trend was observed for Zn and Fe content in grain (42.29 and 86.51 mg kg−1, respectively). For nutrient uptake, ZFN treatment showed the highest uptake of Zn and Fe in grain (604.49 and 1226.22 g ha−1, respectively) and straw (729.55 and 9184.67 g ha−1, respectively). Thus, nano-fertilizers, due to their altered structural properties, demonstrated higher translocation over the mineral and chelated forms of nutrient fertilizers and thus improved yield and nutrient content to a greater extent. Thus, the foliar application of 0.5% ZnO NPs + 0.5% Fe2O3 NPs may prove to be a feasible option for the enrichment of chickpeas with Zn and Fe to ameliorate malnutrition in burgeoning human populations.
A Greener HPTLC Approach for the Determination of β-Carotene in Traditional and Ultrasound-Based Extracts of Different Fractions of Daucus carota (L.), Ipomea batatas (L.), and Commercial Formulation
Authors: Alqarni Mohammed H. , Alam Prawez , Alam Aftab , Ali Abuzer , Foudah Ahmed I. , Alshehri Sultan , Ghoneim Mohammed M. , Shakeel Faiyaz
Published: November 2021

Various analytical approaches for determining β-carotene in vegetable crops and commercial dosage forms have been documented. However, neither the qualitative nor quantitative environmental safety and greener aspects of the literature analytical methodologies of β-carotene analysis have been assessed. As a result, the goal of this research is to develop and validate a reversed-phase “high-performance thin-layer chromatography (HPTLC)” approach for determining β-carotene in traditional (TE) and ultrasound-assisted (UBE) extracts of different fractions of Daucus carota (L.), Ipomea batatas (L.), and commercial formulation. The greener mobile phase for β-carotene analysis was a ternary mixture of ethanol, cyclohexane, and ammonia (95:2.5:2.5, v v v−1). The detection of β-carotene was done at a wavelength of 459 nm. In the 25–1000 ng band−1 range, the greener reversed-phase HPTLC approach was linear. Other validation factors for β-carotene analysis, including as accuracy, precision, robustness, and sensitivity, were likewise dependable. The contents of β-carotene were found to be maximum in hexane: acetone (50:50%) fractions of TE and UBE of D. carota and I. batatas compared to their acetone and hexane fractions. The amount of β-carotene in hexane: acetone (50:50%) portions of TE of D. carota, I. batatas and commercial formulation A was estimated to be 10.32, 3.73, and 6.73 percent w w−1, respectively. However, the amount of β-carotene in hexane: acetone (50:50%) portions of UBE of D. carota, I. batatas and commercial formulation A was estimated to be 11.03, 4.43, and 6.89 percent w w−1, respectively. The greenness scale for the proposed HPTLC strategy was calculated as 0.81 using the “analytical GREEnness (AGREE)” method, indicating that the proposed HPTLC methodology has good greenness. The UBE approach for extracting β-carotene outperformed the TE procedure. These results indicated that the greener reversed-phase HPTLC approach can be utilized for the determination of β-carotene in different vegetable crops, plant-based phytopharmaceuticals, and commercial products. In addition, this approach is also safe and sustainable due to the utilization of a greener mobile phase compared to the toxic mobile phases utilized in literature analytical approaches of β-carotene estimation.
Effect of Betaine Diet on Growth Performance, Carcass Quality and Fat Deposition in Finishing Ningxiang Pigs
Authors: Wang Yaodong , Chen Jiayi , Ji Yingli , Lin Xue , Zhao Yurong
Published: November 2021

The present study was conducted to investigate the effects of diet with betaine supplementation on the growth performance, carcass quality and fat deposition in finishing Ningxiang pigs. A total of 24 Ningxiang pigs (43.6 ± 5.34 kg of average body weight) was randomly divided into two groups, with 6 replicates per treatment and 2 pigs per replicate. The treatments included a control group (basal diet) and a test group (basal diet + 0.2% betaine). The whole trial lasted 81 days. At the end of the experiment, one pig (close to the average body weight of all experimental pigs) per replicate was slaughtered to determine the carcass traits, meat quality and the mRNA expression levels of genes relate to fat deposition (one pig per replicate was randomly selected and fasted for 12 h, n = 6). Results indicated that growth performance was not changed with betaine supplementation. However, dietary with betaine supplementation decreased back fat thickness and fat percentage, and increased the lean meat percentage as well (p < 0.05). In addition, diet with betaine supplementation reduced drip loss, water loss, cooking loss, shear force and b × 24 h value of meat (p < 0.05). There was no difference in total moisture, ether extract and crude protein of longissimus thoracis between the control and test group. Dietary with betaine supplementation decreased ether extract and total cholesterol (p < 0.05) in liver. Dietary with betaine supplementation upregulated the mRNA expression levels of adipose triglyceride lipase (ATGL) and sirtuin 1 (Sirt1), while downregulated the mRNA expression levels of fatty acid synthase (FAS) and acetyl CoA carboxylase (ACC) in subcutaneous fat of back (p < 0.05). Besides, dietary with betaine supplementation upregulated the fatty acid binding protein 4 (FABP4) mRNA expression of longissimus thoracis in finishing Ningxiang pigs (p < 0.05). These results showed that diet supplemented with betaine could improve the slaughtering performance and meat quality, and regulate the genes expression to affect the fat deposition in finishing Ningxiang pigs.
Newborns with Bloody Stools—At the Crossroad between Efficient Management of Necrotizing Enterocolitis and Antibiotic Stewardship
Authors: Heyne-Pietschmann Marie , Lehnick Dirk , Spalinger Johannes , Righini-Grunder Franziska , Buettcher Michael , Lehner Markus , Stocker Martin
Published: November 2021

The onset of bloody stools in neonates often results in antibiotic treatment for suspected necrotizing enterocolitis (NEC). Food protein-induced allergic proctocolitis (FPIAP) is an often-neglected differential diagnosis. We performed a retrospective analysis of antibiotic exposure at our tertiary center from 2011 to 2020 that included three time periods of differing antimicrobial stewardship goals. We compared these data with the conventional treatment guidelines (modified Bell’s criteria). In our cohort of 102 neonates with bloody stools, the length of antibiotic exposure was significantly reduced from a median of 4 to 2 days. The proportion of treated neonates decreased from 100% to 55% without an increase in negative outcomes. There were 434 antibiotic days. Following a management strategy according to modified Bell’s criteria would have led to at least 780 antibiotic days. The delayed initiation of antibiotic treatment was observed in 7 of 102 cases (6.9%). No proven NEC case was missed. Mortality was 3.9%. In conclusion, with FPIAP as a differential diagnosis of NEC, an observational management strategy in neonates with bloody stools that present in a good clinical condition seems to be justified. This may lead to a significant reduction of antibiotic exposure. Further prospective, randomized trials are needed to prove the safety of this observational approach.
Estimates of Antibacterial Consumption in Timor-Leste Using Distribution Data and Variation in Municipality Usage Patterns
Authors: Harris Lisa , Bongers Alexander , Yan Jennifer , Francis Joshua R , Marr Ian , Lake Susanna , Martins Santana
Published: November 2021

The association between antimicrobial resistance and antimicrobial usage has become a growing global concern. Many lower-middle income countries including Timor-Leste (TL) have limited information on antimicrobial usage, although recent research suggests increasing resistance rates among human pathogens there. The aim of this study was to use distribution data to estimate antibiotic consumption at both the national and sub-national level in Timor-Leste, stratifying into resistance class and adherence to the national essential medicines list (EML) and WHO AWaRe guidelines. A retrospective review of distribution data from Timor-Leste central medical store (SAMES) was undertaken to give a defined daily dose (DDD)/1000 inhabitants/day using WHO methodology. National antibiotic distribution in the TL EML in 2019 was estimated at 11.1 DDD/1000 inhabitants/day, comparable to consumption rates observed in other lower-middle-income countries using similar methodology. Differences in distribution quantities were noted between municipalities, with 4 of the 13 municipalities notably above the national average and around 32% of listed restricted antimicrobials distributed incongruent with the EML. This study provides insights into estimated antimicrobial consumption in Timor-Leste that has previously been poorly defined. Estimates of consumption can be used to understand emerging resistance in this small island nation, add to the body of knowledge on antimicrobial use to advise policy and guideline development, and help with stewardship activities.
Impact of Heat Stress on Meat Quality and Antioxidant Markers in Iberian Pigs
Authors: Pardo Zaira , Fernández-Fígares Ignacio , Lachica Manuel , Lara Luis , Nieto Rosa , Seiquer Isabel
Published: November 2021

Heat stress is associated with impaired meat quality and disruption of redox balance. This study investigated the effect of chronic exposure to high temperature on meat quality and antioxidant markers of muscles (longissimus lumborum and gluteus medius) of growing Iberian pigs. Twenty-four pure Iberian pigs were allocated during 28 days to one of three treatments (n = 8/treatment): thermoneutral conditions (20 °C) and ad libitum feeding (TN), heat stress conditions (30 °C) and ad libitum feeding (HS) and thermoneutral and pair-fed with HS (TN-pf). Muscles of the HS group had greater intramuscular fat content than the TN-pf group and higher Zn levels than TN and TN-pf, whereas differences on fatty acid composition were negligible. Heat exposure did not affect pH, color coordinates of redness (a*) and yellowness (b*) and MDA values but had a positive influence on lightness and drip losses. Moreover, chronic heat stress stimulated the activity of antioxidant defenses SOD, CAT and GPx. The statistical factor analysis adequately classified the muscles studied, but was unable to differentiate samples according with treatments. Findings of the present study support an adaptive response of the Iberian pig to high temperatures and show the high Iberian meat quality even under adverse climate situations.
ROS-Induced mtDNA Release: The Emerging Messenger for Communication between Neurons and Innate Immune Cells during Neurodegenerative Disorder Progression
Authors: Zhao Yuanxin , Liu Buhan , Xu Long , Yu Sihang , Fu Jiaying , Wang Jian , Yan Xiaoyu , Su Jing
Published: November 2021

One of the most striking hallmarks shared by various neurodegenerative diseases, including Parkinson’s disease, Alzheimer’s disease and amyotrophic lateral sclerosis, is microglia-mediated and astrocyte-mediated neuroinflammation. Although inhibitions of both harmful proteins and aggregation are major treatments for neurodegenerative diseases, whether the phenomenon of non-normal protein or peptide aggregation is causally related to neuronal loss and synaptic damage is still controversial. Currently, excessive production of reactive oxygen species (ROS), which induces mitochondrial dysfunction in neurons that may play a key role in the regulation of immune cells, is proposed as a regulator in neurological disorders. In this review, we propose that mitochondrial DNA (mtDNA) release due to ROS may act on microglia and astrocytes adjacent to neurons to induce inflammation through activation of innate immune responses (such as cGAS/STING). Elucidating the relationship between mtDNA and the formation of a pro-inflammatory microenvironment could contribute to a better understanding of the mechanism of crosstalk between neuronal and peripheral immune cells and lead to the development of novel therapeutic approaches to neurodegenerative diseases.
Oxidative Stress and Inflammation Caused by Cisplatin Ototoxicity
Authors: Ramkumar Vickram , Mukherjea Debashree , Dhukhwa Asmita , Rybak Leonard P.
Published: November 2021

Hearing loss is a significant health problem that can result from a variety of exogenous insults that generate oxidative stress and inflammation. This can produce cellular damage and impairment of hearing. Radiation damage, ageing, damage produced by cochlear implantation, acoustic trauma and ototoxic drug exposure can all generate reactive oxygen species in the inner ear with loss of sensory cells and hearing loss. Cisplatin ototoxicity is one of the major causes of hearing loss in children and adults. This review will address cisplatin ototoxicity. It includes discussion of the mechanisms associated with cisplatin-induced hearing loss including uptake pathways for cisplatin entry, oxidative stress due to overpowering antioxidant defense mechanisms, and the recently described toxic pathways that are activated by cisplatin, including necroptosis and ferroptosis. The cochlea contains G-protein coupled receptors that can be activated to provide protection. These include adenosine A1 receptors, cannabinoid 2 receptors (CB2) and the Sphingosine 1-Phosphate Receptor 2 (S1PR2). A variety of heat shock proteins (HSPs) can be up-regulated in the cochlea. The use of exosomes offers a novel method of delivery of HSPs to provide protection. A reversible MET channel blocker that can be administered orally may block cisplatin uptake into the cochlear cells. Several protective agents in preclinical studies have been shown to not interfere with cisplatin efficacy. Statins have shown efficacy in reducing cisplatin ototoxicity without compromising patient response to treatment. Additional clinical trials could provide exciting findings in the prevention of cisplatin ototoxicity.
Real Driving Cycle-Based State of Charge Prediction for EV Batteries Using Deep Learning Methods
Authors: Hong Seokjoon , Hwang Hoyeon , Kim Daniel , Cui Shengmin , Joe Inwhee
Published: November 2021

An accurate prediction of the State of Charge (SOC) of an Electric Vehicle (EV) battery is important when determining the driving range of an EV. However, the majority of the studies in this field have either been focused on the standard driving cycle (SDC) or the internal parameters of the battery itself to predict the SOC results. Due to the significant difference between the real driving cycle (RDC) and SDC, a proper method of predicting the SOC results with RDCs is required. In this paper, RDCs and deep learning methods are used to accurately estimate the SOC of an EV battery. RDC data for an actual driving route have been directly collected by an On-Board Diagnostics (OBD)-II dongle connected to the author’s vehicle. The Global Positioning System (GPS) data of the traffic lights en route are used to segment each instance of the driving cycles where the Dynamic Time Warping (DTW) algorithm is adopted, to obtain the most similar patterns among the driving cycles. Finally, the acceleration values are predicted from deep learning models, and the SOC trajectory for the next trip will be obtained by a Functional Mock-Up Interface (FMI)-based EV simulation environment where the predicted accelerations are fed into the simulation model by each time step. As a result of the experiments, it was confirmed that the Temporal Attention Long–Short-Term Memory (TA-LSTM) model predicts the SOC more accurately than others.
Live-Scale Testing of Granular Materials Stabilized with Alkali-Activated Waste Glass and Carbide Lime
Authors: Secco Marina Paula , Mesavilla Débora Thaís , Floss Márcio Felipe , Cesar Consoli Nilo , Miranda Tiago , Cristelo Nuno
Published: November 2021

The increasingly strong search for alternative materials to Portland cement has resulted in the development of alkali-activated cements (AAC) that are very effective at using industrial by-products as raw materials, which also contributes to the volume reduction in landfilled waste. Several studies targeting the development of AAC—based on wastes containing silicon and calcium—for chemical stabilization of soils have demonstrated their excellent performance in terms of durability and mechanical performance. However, most of these studies are confined to a laboratory characterization, ignoring the influence and viability of the in situ construction process and, also important, of the in situ curing conditions. The present work investigated the field application of an AAC based on carbide lime and glass wastes to stabilize fine sand acting as a superficial foundation. The assessment was supported on the unconfined compressive strength (UCS) and initial shear modulus (G0) of the developed material, and the field results were compared with those prepared in the laboratory, up to 120 days curing. In situ tests were also developed on the field layers (with diameters of 450 and 900 mm and thickness of 300 mm) after different curing times. To establish a reference, the mentioned precursors were either activated with a sodium hydroxide solution or hydrated with water (given the reactivity of the lime). The results showed that the AAC-based mixtures developed greater strength and stiffness at a faster rate than the water-based mixtures. Specimens cured under controlled laboratory conditions showed better results than the samples collected in the field. The inclusion of the stabilized layers clearly increased the load-bearing capacity of the natural soil, while the different diameters produced different failure mechanisms, similar to those found in Portland cement stabilization.
Physiological Effects of Green-Colored Food-Derived Bioactive Compounds on Cancer
Authors: Al Mijan Mohammad , Sim Woo-Jin , Lim Tae-Gyu
Published: November 2021

Green-colored foods, such as broccoli, sprouts, soybean, and green leafy vegetables are considered one of the representative healthy foods for containing various functional ingredients that can combat chronic diseases, including diabetes, obesity, and cancer. Herein, we reviewed the anti-cancer activities and the underlying mechanisms of some important bioactive compounds, such as sulforaphane, catechins, chlorophyll, isoflavone, indole dervatives, and lutein, present in green-colored foods. In vivo and clinical studies suggest that sulforaphane, a sulfur-containing compound found in cruciferous vegetables, can ameliorate prostate and breast cancer symptoms by arresting cell-cycle progression and modulating Ki67 and HDAC expression. A green tea compound, known as epigallocatechin-3-gallate (EGCG), has shown remarkable anti-cancer effects against prostate cancer and lung adenocarcinoma in human trials through its antioxidative defense and immunomodulatory functions. Chlorophyll, a natural pigment found in all green plants, can regulate multiple cancer-related genes, including cyclin D1, CYP1A, CYP1B1, and p53. Epidemiological studies indicate that chlorophyll can substantially reduce aflatoxin level and can mitigate colon cancer in human subjects. Remarkably, the consumption of soy isoflavone has been found to be associated with the lower incidence and mortality of breast and prostate cancers in East Asia and in Canada. In vivo and in vitro data point out that isoflavone has modulatory effects on estrogen and androgen signaling pathways and the expression of MAPK, NfκB, Bcl-2, and PI3K/AKT in different cancer models. Other green food bioactive compounds, such as indole derivatives and lutein, also exhibited suppressing effects in rodent models of lung, liver, stomach, cervical, and prostate cancers. In addition, some micronutrients, such as folate, riboflavin, retinoic acid, and vitamin D3 present in green foods, also showed potential cancer suppressing effects. Taken together, these data suggest potential chemopreventive functions of the bioactive compounds from green-colored foods. This paper could be beneficial for further research on the anti-carcinogenic effects of green-colored food-derived compounds, in order to develop green chemotherapeutics for cancers.
Reduced Graphene Oxide-Metalloporphyrin Sensors for Human Breath Screening
Authors: Lee Bo Mi , Eetemadi Ameen , Tagkopoulos Ilias
Published: November 2021

The objective of this study is to validate reduced graphene oxide (RGO)-based volatile organic compounds (VOC) sensors, assembled by simple and low-cost manufacturing, for the detection of disease-related VOCs in human breath using machine learning (ML) algorithms. RGO films were functionalized by four different metalloporphryins to assemble cross-sensitive chemiresistive sensors with different sensing properties. This work demonstrated how different ML algorithms affect the discrimination capabilities of RGO–based VOC sensors. In addition, an ML-based disease classifier was derived to discriminate healthy vs. unhealthy individuals based on breath sample data. The results show that our ML models could predict the presence of disease-related VOC compounds of interest with a minimum accuracy and F1-score of 91.7% and 83.3%, respectively, and discriminate chronic kidney disease breath with a high accuracy, 91.7%.
Experimental Determination of Corneal Elastic Constants and Their Use in Biomechanical Modeling
Authors: Muñoz-Villaescusa Claudia , Núñez-Chongo Osiris de la C. , Cárdenas-Díaz Taimi , Batista-Leyva Alfo J. , Cavas-Martínez Francisco
Published: November 2021

Corneal biomechanics aims to establish the physico-mathematical bases that allow for predicting the corneal response to physiological and pathological situations by creating models of tissue behavior. Determining the characteristic parameters of these models is a formidable challenge in the biomechanical modeling process. To contribute to corneal tissue characterization, an experimental set-up was designed, built and tested to study corneal behavior by applying changes in pressure. The elastic constants of porcine corneas were determined, and a Young’s modulus of 0.188 MPa and 26.22% hysteresis were obtained. A computational cornea model was developed to analyze the influence of different factors. Minor variations in the applied conditions were found for apical displacement and pachymetry, and the corneal behavior was reproduced. However, the optical power behavior was affected by variations in the applied conditions, and the experimentally obtained data could not be reproduced. Despite its importance, this parameter has not been analyzed in-depth by other studies, which shows that the quality of a biomechanical cornea model should not be evaluated only by apical displacement.