bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of the Distance between the Measured and Assumed Location of a Point Source of Pollution in Groundwater as a Function of the Variance of the Estimation Error
Authors: Kovač Ivan , Šrajbek Marko , Klišanin Nikolina , Gilja Gordon
Published: October 2023

The localization of pollution sources is one of the main tasks in environmental engineering. For this paper, models of spatial distribution of nitrate concentration in groundwater were created, and the point of highest concentration was determined. This point represents the assumed location of the pollution source and differs from the actual location, so there is a certain distance between the measured and assumed location. This paper puts forward a new hypothesis that the distance between the measured and the assumed location is a function of the variance of the estimation error. The scientific contribution of this paper is based on the fact that the interaction of statistical and geostatistical methods can locate the dominant point source of pollution or narrow down the search area. The above hypothesis is confirmed by the example of the Varaždin wellfield, which was closed due to an excessively high groundwater nitrate concentration. Seven different interpolation methods were used to create spatial distribution models. Each method provides a different model, a different variance of the estimation error, and estimates of the location of the pollution source. The smallest value of variance of the estimation error of 1.65 was obtained for the minimum curvature interpolation method and the largest value of variance (24.49) was obtained for the kriging with logarithmic variogram. Our results show a nonlinear and monotonic relationship between the distance and the variance of the estimation error, so logarithmic and rational quadratic models were fitted to the scatter point data. The models were linearized, a t-test was performed, and the results show that the models can be considered reliable, which is confirmed by the values of the coefficients of determination of the linearized models, which are around 0.50. The obtained results can be used in planning additional research work to determine the measured location of the pollution source. The research methodology we used is universal and can be applied to other locations where high concentrations of certain contaminants have been detected in groundwater in alluvial aquifers.
Medical Care Management Based on Disaster Medicine for the Triathlon Events at the XXXII Olympiad and Tokyo 2020 Paralympic Games
Authors: Yagi Masaharu , Kasanami Ryoji , Tarumi Yoko , Dohi Kenji
Published: October 2023

Planning the medical services for the triathlon competition at the 2020 Tokyo Olympic and Paralympic Games was predicted to be challenging because of possible last-minute changes related to the COVID-19 pandemic and abnormally high temperatures. Therefore, event planners organized and executed a disaster medical care plan, a plan for providing care during emergencies. Based on the basic medical plan for all venues provided by the Tokyo 2020 Organizing Committee, planners for the triathlon venue prepared a medical care plan according to the CSCATTT principles: Command and control, Safety, Communication, Assessment, Treatment, Triage, and Transport. After the event, planners evaluated the number of COVID-19, heatstroke, and injury cases at the venue. The events were conducted without spectators in July and August 2022 because at the last minute, planners held the event without spectators. The triathlon competition involved 638 individuals, including athletes and staff. In total, 7 cases of injuries, 3 cases of mild heatstroke, and 13 other cases were reported, with only 2 requiring emergency transportation. No cases of COVID-19 were reported from the triathlon venue, including during the observation period after the event. This medical plan was effective in preventing heatstroke and COVID-19 cases during the big event. Efficiently and effectively responding to various situations is possible in a shorter period by planning large-scale medical services for such special circumstances according to CSCATTT, a principle of disaster medical care.
Responding to the HIV Health Literacy Needs of Clients in Substance Use Treatment: The Role of Universal PrEP Education in HIV Health and Prevention
Authors: Zhai Yusen , Isadore Kyesha M. , Parker Lauren , Sandberg Jeremy
Published: October 2023

Health literacy, particularly HIV health literacy, is a key social determinant of health and can be significantly improved through targeted health education. This paper explores the often-overlooked potential of pre-exposure prophylaxis (PrEP) education as a powerful tool to enhance HIV health literacy among people with substance use disorders (PWSUD), a population notably susceptible to HIV. Given the syndemic interplay of substance use disorders (SUDs) and HIV, health professionals, especially substance use counselors, are uniquely positioned to bolster HIV health literacy and positively influence health outcomes. This article offers a brief introduction to PrEP, delineates potential barriers and facilitators to its use and education, and proposes strategies for effective PrEP education, implementation, and adherence. By equipping substance use counselors with essential knowledge and skills, we aim to encourage and promote the integration of PrEP education into substance use treatment. The overarching objective is to empower counselors to proactively engage in HIV prevention efforts, thereby fulfilling pressing health literacy needs and contributing to improved health outcomes among PWSUD.
Situating Stigma: An Ethnographic Exploration of How Stigma Arises in Interactions at Different Stages of People’s Drug Use Journeys
Authors: Long Fiona Catherine , Jepsen Kirsty Stuart
Published: October 2023

The association between stigma and drug use has been widely researched. However, to fully understand the nuances of stigma, as experienced by people who use drugs (PWUD), it is argued that we must look at the situations within which stigma is encountered. To obtain an ‘up close’ look at situated stigma, data are drawn from two ethnographic studies—one set in a homeless hostel in the South of England and the other at a substance use service in South Wales. This article explores how PWUD experience and negotiate stigma at different stages of their drug use. We identify three notable themes across these settings. Firstly, ‘othering’ occurs in two distinct ways, by othering the past self or distancing from other PWUDs. Secondly, ‘drug exceptionalism’ is used to justify an individual’s drug use and express frustration at the associations between legality, social harm, and stigma. Finally, in ‘negotiating identities’, individuals present alternate identity roles to either demonstrate clashes in identity or to promote a conventionally positive sense of self. This article contributes to the existing literature on stigma, firstly, by comparing the stigma management strategies of those in active drug use and recovery and, secondly, by using this to highlight the importance of ethnography and situated stigma within this field.
The Protective Role of Group Activity Prior to COVID-19 Pandemic Quarantine on the Relation between Loneliness and Quality of Life during COVID-19 Pandemic
Authors: Vitman Schorr Adi , Yehuda Itamar , Mor Ron
Published: October 2023

Background: Studies of aging have suggested that QoL is positively associated with active social contacts and supportive face to face social networks. However, social networks and contacts in later life decrease due to a variety of reasons; the narrowing of social networks contributes to increased social isolation and loneliness and leads to the deterioration of well-being and QoL among adult persons. The goal of this study was to explore the relationship between loneliness feelings and QoL during the COVID-19 quarantine as potentially moderated by group physical activity prior to the COVID-19 pandemic quarantine. Methods: A convenience sample of 99 older adults aged 60 and over was interviewed. Using bootstrapping, we tested the strength and significance of the conditional moderation effect of group physical activity prior to the COVID-19 pandemic quarantine on the relationship between loneliness feelings and QoL during the COVID-19 quarantine. Results: the results demonstrated a direct negative effect between loneliness feelings and QoL during the COVID-19 pandemic and that the relationship was moderated by group physical activity prior to the COVID-19 pandemic quarantine (p = 0.000). Conclusion: the findings indicate that policy makers and professionals working with older adults should seek ways to attract older adults to participate in group physical activity and enjoy its long-term social benefits.
Could Necroleachate Be the Cemetery’s Sewage? A Panorama from Brazilian Legislation
Authors: Azevedo Ana Paula Chein Bueno de , Cardoso Telma Abdalla de Oliveira , Cohen Simone Cynamon
Published: October 2023

Cemeteries can be compared to landfills, as the leachate produced in these areas, also known as necroleachate, can be environmentally transported, polluting groundwater, surface water, and soil. In Brazil, to ensure no negative environmental impacts and public health risks, cemetery management is the responsibility of states and municipalities. In this context, this article aims to discuss Brazilian sanitary–environmental legislation concerning cemetery waste management. Only half of all Brazilian states have established sanitary–environmental cemetery legislation, and only 19 municipalities have specific laws. These laws, however, are broad and contain many gaps. Necroleachate care and control require both sanitary and environmental assessments to avoid environmental vulnerability and contamination risks for populations inhabiting surrounding areas. In this regard, new water analysis parameters in environmentally vulnerable areas should be established to control the population’s drinking water quality, such as the detection of C. perfringens. Furthermore, the construction of vertical cemeteries instead of horizontal ones and the adoption of cremation procedures should also be considered. This assessment comprises a novel research framework, as no studies on the impact of Brazilian laws on environmental necroleachate contamination are available to date.
Perioperative Nutritional Management in Enhanced Recovery after Bariatric Surgery
Authors: Flore Giovanna , Deledda Andrea , Fosci Michele , Lombardo Mauro , Moroni Enrico , Pintus Stefano , Velluzzi Fernanda , Fantola Giovanni
Published: October 2023

Obesity is a crucial health problem because it leads to several chronic diseases with an increased risk of mortality and it is very hard to reverse with conventional treatment including changes in lifestyle and pharmacotherapy. Bariatric surgery (BS), comprising a range of various surgical procedures that modify the digestive tract favouring weight loss, is considered the most effective medical intervention to counteract severe obesity, especially in the presence of metabolic comorbidities. The Enhanced Recovery After Bariatric Surgery (ERABS) protocols include a set of recommendations that can be applied before and after BS. The primary aim of ERABS protocols is to facilitate and expedite the recovery process while enhancing the overall effectiveness of bariatric procedures. ERABS protocols include indications about preoperative fasting as well as on how to feed the patient on the day of the intervention, and how to nourish and hydrate in the days after BS. This narrative review examines the application, the feasibility and the efficacy of ERABS protocols applied to the field of nutrition. We found that ERABS protocols, in particular not fasting the patient before the surgery, are often not correctly applied for reasons that are not evidence-based. Furthermore, we identified some gaps in the research about some practises that could be implemented in the presence of additional evidence.
Reply to Chaudhuri et al. Comment on “Balwierz et al. Potential Carcinogens in Makeup Cosmetics. Int. J. Environ. Res. Public Health 2023, 20, 4780”
Authors: Balwierz Radosław , Biernat Paweł , Jasińska-Balwierz Agata , Siodłak Dawid , Kusakiewicz-Dawid Anna , Kurek-Górecka Anna , Olczyk Paweł , Ochędzan-Siodłak Wioletta
Published: October 2023

Comments by Chaudhuri et al. (2023) [...]
Tailoring Properties of Hyaluronate-Based Core–Shell Nanocapsules with Encapsulation of Mixtures of Edible Oils
Authors: Bednorz Justyna , Smela Krzysztof , Zapotoczny Szczepan
Published: October 2023

Dispersions of core–shell nanocapsules (nanoemulsion) composed of liquid oil cores and polysaccharide-based shells were fabricated with emulsification using various mixtures of edible oils and amphiphilic hyaluronate derivatized with 12-carbon alkyl chains forming the shells. Such nanocapsules, with typical diameters in the 100–500 nm range, have been previously shown as promising carriers of lipophilic bioactive compounds. Here, the influence of some properties of the oil cores on the size and stability of the capsules were systematically investigated using oil binary mixtures. The results indicated that, in general, the lower the density, viscosity, and interfacial tension (IFT) between the oil and aqueous polymer solution phases, the smaller the size of the capsules. Importantly, an unexpected synergistic reduction of IFT of mixed oils was observed leading to the values below the measured for individual oils. Such a behavior may be used to tailor size but also other properties of the nanocapsules (e.g., stability, solubility of encapsulated compounds) that could not be achieved applying just a single oil. It is in high demand for applications in pharmaceutical or food industries and opens opportunities of using more complex combinations of oils with more components to achieve an even further reduction of IFT leading to even smaller nanocapsules.
Trichinella spiralis-Secreted Products Promote Collagen Capsule Formation through TGF-β1/Smad3 Pathway
Authors: Cheng Ge , Zhang Zifang , Wang Yixuan , Zao Youjiao , Wang Ruoqi , Gao Mengying , Feng Miaomiao , Zhang Xi , Jiang Peng
Published: October 2023

Trichinella spiralis (T. spiralis) muscle larvae colonize in the host’s skeletal muscle cells, which are surrounded by collagen capsules. The mechanism underlying muscle stage larva-induced collagen capsule formation remains unknown. To clarify the mechanism, a T. spiralis muscular-infected mouse model was established by a single lateral tail vein injection with 20,000 T. spiralis newborn larvae (NBL). The infected mice were treated with or without SB525334 (TGF-β1 receptor type I inhibitor). Diaphragms were obtained post-infection, and the expression levels of the TGF-β1/Smad3 pathway-related genes and collagen genes (type IV and VI) were observed during the process of collagen capsule formation. The changes in myoblasts under stimulation of the excretory–secretory (ES) products of NBL with or without SB525334 were further investigated. Results showed that the expression levels of type IV collagen gene, type VI collagen gene, Tgfb1, and Smad3 were significantly increased in infected mice muscle cells. The expression levels of all the above genes were enhanced by the products of NBL in myoblast cells. These changes were reversed by co-treatment with SB525334 in vivo and in vitro. In conclusion, the TGF-β1/Smad3 pathway can be activated by T. spiralis infection in muscle cells. The activated TGF-β1/Smad3 pathway can stimulate the secretion of collagens by myocytes and plays a promoting role in the process of collagen capsule formation. The research has the limitation that the protein identification of the products of NBL has yet to be performed. Therefore, the specific components in the T. spiralis ES products that induce collagen synthesis should be further investigated.
Insulin Receptor Isoforms and Insulin Growth Factor-like Receptors: Implications in Cell Signaling, Carcinogenesis, and Chemoresistance
Authors: Galal Mariam Ahmed , Alouch Samhar Samer , Alsultan Buthainah Saad , Dahman Huda , Alyabis Nouf Abdullah , Alammar Sarah Ammar , Aljada Ahmad
Published: October 2023

This comprehensive review thoroughly explores the intricate involvement of insulin receptor (IR) isoforms and insulin-like growth factor receptors (IGFRs) in the context of the insulin and insulin-like growth factor (IGF) signaling (IIS) pathway. This elaborate system encompasses ligands, receptors, and binding proteins, giving rise to a wide array of functions, including aspects such as carcinogenesis and chemoresistance. Detailed genetic analysis of IR and IGFR structures highlights their distinct isoforms, which arise from alternative splicing and exhibit diverse affinities for ligands. Notably, the overexpression of the IR-A isoform is linked to cancer stemness, tumor development, and resistance to targeted therapies. Similarly, elevated IGFR expression accelerates tumor progression and fosters chemoresistance. The review underscores the intricate interplay between IRs and IGFRs, contributing to resistance against anti-IGFR drugs. Consequently, the dual targeting of both receptors could present a more effective strategy for surmounting chemoresistance. To conclude, this review brings to light the pivotal roles played by IRs and IGFRs in cellular signaling, carcinogenesis, and therapy resistance. By precisely modulating these receptors and their complex signaling pathways, the potential emerges for developing enhanced anti-cancer interventions, ultimately leading to improved patient outcomes.
PNA6, a Lactosyl Analogue of Angiotensin-(1-7), Reverses Pain Induced in Murine Models of Inflammation, Chemotherapy-Induced Peripheral Neuropathy, and Metastatic Bone Disease
Authors: Sulaiman Maha I. , Alabsi Wafaa , Szabo Lajos , Hay Meredith , Polt Robin , Largent-Milnes Tally M. , Vanderah Todd W.
Published: October 2023

Pain is the most significant impairment and debilitating challenge for patients with bone metastasis. Therefore, the primary objective of current therapy is to mitigate and prevent the persistence of pain. Thus, cancer-induced bone pain is described as a multifaceted form of discomfort encompassing both inflammatory and neuropathic elements. We have developed a novel non-addictive pain therapeutic, PNA6, that is a derivative of the peptide Angiotensin-(1-7) and binds the Mas receptor to decrease inflammation-related cancer pain. In the present study, we provide evidence that PNA6 attenuates inflammatory, chemotherapy-induced peripheral neuropathy (CIPN) and cancer pain confined to the long bones, exhibiting longer-lasting efficacious therapeutic effects. PNA6, Asp-Arg-Val-Tyr-Ile-His-Ser-(O-β-Lact)-amide, was successfully synthesized using solid phase peptide synthesis (SPPS). PNA6 significantly reversed inflammatory pain induced by 2% carrageenan in mice. A second murine model of platinum drug-induced painful peripheral neuropathy was established using oxaliplatin. Mice in the oxaliplatin-vehicle treatment groups demonstrated significant mechanical allodynia compared to the oxaliplatin-PNA6 treatment group mice. In a third study modeling a complex pain state, E0771 breast adenocarcinoma cells were implanted into the femur of female C57BL/6J wild-type mice to induce cancer-induced bone pain (CIBP). Both acute and chronic dosing of PNA6 significantly reduced the spontaneous pain behaviors associated with CIBP. These data suggest that PNA6 is a viable lead candidate for treating chronic inflammatory and complex neuropathic pain.
Integrated Multi-Omics Analyses Reveal That Autophagy-Mediated Cellular Metabolism Is Required for the Initiation of Pollen Germination
Authors: Zhou Xuemei , Zhang Qiuyu , Zhao Yuliang , Ding Shanshan , Yu Guang-Hui
Published: October 2023

Autophagy is an evolutionarily conserved mechanism for degrading and recycling various cellular components, functioning in both normal development and stress conditions. This process is tightly regulated by a set of autophagy-related (ATG) proteins, including ATG2 in the ATG9 cycling system and ATG5 in the ATG12 conjugation system. Our recent research demonstrated that autophagy-mediated compartmental cytoplasmic deletion is essential for pollen germination. However, the precise mechanisms through which autophagy regulates pollen germination, ensuring its fertility, remain largely unknown. Here, we applied multi-omics analyses, including transcriptomic and metabolomic approaches, to investigate the downstream pathways of autophagy in the process of pollen germination. Although ATG2 and ATG5 play similar roles in regulating pollen germination, high-throughput transcriptomic analysis reveals that silencing ATG5 has a greater impact on the transcriptome than silencing ATG2. Cross-comparisons of transcriptome and proteome analysis reveal that gene expression at the mRNA level and protein level is differentially affected by autophagy. Furthermore, high-throughput metabolomics analysis demonstrates that pathways related to amino acid metabolism and aminoacyl-tRNA biosynthesis were affected by both ATG2 and ATG5 silencing. Collectively, our multi-omics analyses reveal the central role of autophagy in cellular metabolism, which is critical for initiating pollen germination and ensuring pollen fertility.
Autophagy as a Target for Non-Immune Intrinsic Functions of Programmed Cell Death-Ligand 1 in Cancer
Authors: García-Pérez Blanca Estela , Pérez-Torres Christian , Baltierra-Uribe Shantal Lizbeth , Castillo-Cruz Juan , Castrejón-Jiménez Nayeli Shantal
Published: October 2023

Autophagy is a catabolic process that is essential to the maintenance of homeostasis through the cellular recycling of damaged organelles or misfolded proteins, which sustains energy balance. Additionally, autophagy plays a dual role in modulating the development and progression of cancer and inducing a survival strategy in tumoral cells. Programmed cell death-ligand 1 (PD-L1) modulates the immune response and is responsible for maintaining self-tolerance. Because tumor cells exploit the PD-L1–PD-1 interaction to subvert the immune response, immunotherapy has been developed based on the use of PD-L1-blocking antibodies. Recent evidence has suggested a bidirectional regulation between autophagy and PD-L1 molecule expression in tumor cells. Moreover, the research into the intrinsic properties of PD-L1 has highlighted new functions that are advantageous to tumor cells. The relationship between autophagy and PD-L1 is complex and still not fully understood; its effects can be context-dependent and might differ between tumoral cells. This review refines our understanding of the non-immune intrinsic functions of PD-L1 and its potential influence on autophagy, how these could allow the survival of tumor cells, and what this means for the efficacy of anti-PD-L1 therapeutic strategies.
3β-Hydroxy-12-oleanen-27-oic Acid Exerts an Antiproliferative Effect on Human Colon Carcinoma HCT116 Cells via Targeting FDFT1
Authors: Tu Jue , Meng Xiang , Wang Juanjuan , Han Ziyi , Yu Zuoting , Sun Hongxiang
Published: October 2023

3β-hydroxy-12-oleanen-27-oic acid (ATA), a cytotoxic oleanane triterpenoid with C14-COOH isolated from the rhizome of Astilbe chinensis, has been previously proven to possess antitumor activity and may be a promising antitumor agent. However, its molecular mechanisms of antitumor action were still unclear. This study explored the underlying mechanisms of cytotoxicity and potential target of ATA against human colorectal cancer HCT116 cells via integrative analysis of transcriptomics and network pharmacology in combination with in vitro and in vivo experimental validations. ATA significantly inhibited the proliferation of HCT116 cells in a concentration- and time-dependent manner and induced the cell cycle arrest at the G0/G1 phase, apoptosis, autophagy, and ferroptosis. Transcriptomic analysis manifested that ATA regulated mRNA expression of the genes related to cell proliferation, cell cycle, and cell death in HCT116 cells. The integrated analysis of transcriptomics, network pharmacology, and molecular docking revealed that ATA exerted cytotoxic activity via interactions with FDFT1, PPARA, and PPARG. Furthermore, FDFT1 was verified to be an upstream key target mediating the antiproliferative effect of ATA against HCT116 cells. Of note, ATA remarkably suppressed the growth of HCT116 xenografts in nude mice and displayed an apparent attenuation of FDFT1 in tumor tissues accompanied by the alteration of the biomarkers of autophagy, cell cycle, apoptosis, and ferroptosis. These results demonstrate that ATA exerted in vitro and in vivo antiproliferative effects against HCT116 cells through inducing cell apoptosis, autophagy, and ferroptosis via targeting FDFT1.
The Co-Expression Pattern of Calcium-Binding Proteins with γ-Aminobutyric Acid and Glutamate Transporters in the Amygdala of the Guinea Pig: Evidence for Glutamatergic Subpopulations
Authors: Kalinowski Daniel , Bogus-Nowakowska Krystyna , Kozłowska Anna , Równiak Maciej
Published: October 2023

The amygdala has large populations of neurons utilizing specific calcium-binding proteins such as parvalbumin (PV), calbindin (CB), or calretinin (CR). They are considered specialized subsets of γ-aminobutyric acid (GABA) interneurons; however, many of these cells are devoid of GABA or glutamate decarboxylase. The neurotransmitters used by GABA-immunonegative cells are still unknown, but it is suggested that a part may use glutamate. Thus, this study investigates in the amygdala of the guinea pig relationships between PV, CB, or CR-containing cells and GABA transporter (VGAT) or glutamate transporter type 2 (VGLUT2), markers of GABAergic and glutamatergic neurons, respectively. The results show that although most neurons using PV, CB, and CR co-expressed VGAT, each of these populations also had a fraction of VGLUT2 co-expressing cells. For almost all neurons using PV (~90%) co-expressed VGAT, while ~1.5% of them had VGLUT2. The proportion of neurons using CB and VGAT was smaller than that for PV (~80%), while the percentage of cells with VGLUT2 was larger (~4.5%). Finally, only half of the neurons using CR (~53%) co-expressed VGAT, while ~3.5% of them had VGLUT2. In conclusion, the populations of neurons co-expressing PV, CB, and CR are in the amygdala, primarily GABAergic. However, at least a fraction of neurons in each of them co-express VGLUT2, suggesting that these cells may use glutamate. Moreover, the number of PV-, CB-, and CR-containing neurons that may use glutamate is probably larger as they can utilize VGLUT1 or VGLUT3, which are also present in the amygdala.
Computer Vision Tasks for Ambient Intelligence in Children’s Health
Authors: Germanese Danila , Colantonio Sara , Del Coco Marco , Carcagnì Pierluigi , Leo Marco
Published: October 2023

Computer vision is a powerful tool for healthcare applications since it can provide objective diagnosis and assessment of pathologies, not depending on clinicians’ skills and experiences. It can also help speed-up population screening, reducing health care costs and improving the quality of service. Several works summarise applications and systems in medical imaging, whereas less work is devoted to surveying approaches for healthcare goals using ambient intelligence, i.e., observing individuals in natural settings. Even more, there is a lack of papers providing a survey of works exhaustively covering computer vision applications for children’s health, which is a particularly challenging research area considering that most existing computer vision technologies have been trained and tested only on adults. The aim of this paper is then to survey, for the first time in the literature, the papers covering children’s health-related issues by ambient intelligence methods and systems relying on computer vision.
CapGAN: Text-to-Image Synthesis Using Capsule GANs
Authors: Omar Maryam , Ur Rehman Hafeez , Samin Omar Bin , Alazab Moutaz , Politano Gianfranco , Benso Alfredo
Published: October 2023

Text-to-image synthesis is one of the most critical and challenging problems of generative modeling. It is of substantial importance in the area of automatic learning, especially for image creation, modification, analysis and optimization. A number of works have been proposed in the past to achieve this goal; however, current methods still lack scene understanding, especially when it comes to synthesizing coherent structures in complex scenes. In this work, we propose a model called CapGAN, to synthesize images from a given single text statement to resolve the problem of global coherent structures in complex scenes. For this purpose, skip-thought vectors are used to encode the given text into vector representation. This encoded vector is used as an input for image synthesis using an adversarial process, in which two models are trained simultaneously, namely: generator (G) and discriminator (D). The model G generates fake images, while the model D tries to predict what the sample is from training data rather than generated by G. The conceptual novelty of this work lies in the integrating capsules at the discriminator level to make the model understand the orientational and relative spatial relationship between different entities of an object in an image. The inception score (IS) along with the Fréchet inception distance (FID) are used as quantitative evaluation metrics for CapGAN. IS recorded for images generated using CapGAN is 4.05 ± 0.050, which is around 34% higher than images synthesized using traditional GANs, whereas the FID score calculated for synthesized images using CapGAN is 44.38, which is ab almost 9% improvement from the previous state-of-the-art models. The experimental results clearly demonstrate the effectiveness of the proposed CapGAN model, which is exceptionally proficient in generating images with complex scenes.
Sintilimab-Induced Myocarditis in a Patient with Gastric Cancer: A Case Report and Literature Review
Authors: Liu Xin , Zeng Ziyue , Cao Jianlei , Li Xianqing , Muhetaer Muheremu , Jin Zhili , Cai Huanhuan , Lu Zhibing
Published: October 2023

Immune checkpoint inhibitors (ICIs) have emerged as a powerful and efficacious therapeutic approach for many cancer patients. Sintilimab is a fully human IgG4 monoclonal antibody that binds with programmed cell death receptor-1 (PD-1) to block its interaction with ligands, thereby enhancing the antitumor effects of T cells. However, ICIs may induce immune-related adverse events (irAEs) in various systems and organs, with fulminant myocarditis being the most severe one. We report the case of a 45-year-old female with gastric cancer who developed chest pain two weeks after chemotherapy with sintilimab; she was diagnosed with immune-associated fulminant myocarditis and experienced an Adams–Stokes syndrome attack in the hospital. Eventually, she was discharged after being treated with methylprednisolone, immunoglobulin, and an IABP.
A Framework for Synthetic Agetech Attack Data Generation
Authors: Khaemba Noel , Traoré Issa , Mamun Mohammad
Published: October 2023

To address the lack of datasets for agetech, this paper presents an approach for generating synthetic datasets that include traces of benign and attack datasets for agetech. The generated datasets could be used to develop and evaluate intrusion detection systems for smart homes for seniors aging in place. After reviewing several resources, it was established that there are no agetech attack data for sensor readings. Therefore, in this research, several methods for generating attack data were explored using attack data patterns from an existing IoT dataset called TON_IoT weather data. The TON_IoT dataset could be used in different scenarios, but in this study, the focus is to apply it to agetech. The attack patterns were replicated in a normal agetech dataset from a temperature sensor collected from the Information Security and Object Technology (ISOT) research lab. The generated data are different from normal data, as abnormal segments are shown that could be considered as attacks. The generated agetech attack datasets were also trained using machine learning models, and, based on different metrics, achieved good classification performance in predicting whether a sample is benign or malicious.
Bioinspired Collagen/Hyaluronic Acid/Fibrin-Based Hydrogels for Soft Tissue Engineering: Design, Synthesis, and In Vitro Characterization
Authors: Bindi Bianca , Perioli Annalisa , Melo Priscila , Mattu Clara , Ferreira Ana Marina
Published: October 2023

A major challenge for future drug development comprises finding alternative models for drug screening. The use of animal models in research is highly controversial, with an ongoing debate on their ethical acceptability. Also, animal models are often poorly predictive of therapeutic outcomes due to the differences between animal and human physiological environments. In this study, we aimed to develop a biomimetic hydrogel that replicates the composition of skin for potential use in in vitro modeling within tissue engineering. The hydrogel was fabricated through the crosslinking of collagen type I, hyaluronic acid, four-arm PEG succinimidyl glutarate (4S-StarPEG), and fibrinogen. Various ratios of these components were systematically optimized to achieve a well-interconnected porosity and desirable rheological properties. To evaluate the hydrogel’s cytocompatibility, fibroblasts were embedded within the matrix. The resulting hydrogel exhibited promising properties as a scaffold, also facilitating the growth of and proliferation of the cells. This biomimetic hydrogel holds great potential for tissue engineering applications, particularly in skin regeneration and cancer research. The study used melanoma spheroids fabricated using the 96-round bottom well plate method as a potential application. The results demonstrate that the developed hydrogels allowed the maintenance of spheroid integrity and viability, meaning it has a promising use as a three-dimensional in vitro model of melanoma for both tissue engineering and drug screening applications.
Are Absorbable Plates More Resistant to Infection Than Titanium Implants? An Experimental Pre-Clinical Trial in Rabbits
Authors: Kitridis Dimitrios , Savvidis Panagiotis , Cheva Angeliki , Papalois Apostolos , Givissis Panagiotis , Chalidis Byron
Published: October 2023

Background: Infection of orthopaedic implants after internal fixation of bone fractures remains a major complication with occasionally devastating consequences. Recent studies have reported that the use of absorbable materials, instead of metallic ones, may lead to a lower incidence of postoperative infection. In this experimental pre-clinical animal study, we compared the infection rate between absorbable implants consisting of copolymers composed from trimethylene carbonate, L-polylactic acid, and D, L-polylactic acid monomers, and titanium implants after the inoculation of a pathogenic microorganism. Material and Methods: We used an experimental implant-related infection model in rabbits. Sixty animals were randomly and equally divided into two groups. In all animals, the right femur was exposed via a lateral approach and a 2.5 mm two-hole titanium plate with screws (Group A), or a two-hole absorbable plate and screws (Group B), were applied in the femoral shaft. Afterwards, the implant surface was inoculated with Pseudomonas Aeruginosa at a concentration of 2 × 108 CFU/mL. The primary outcome was the comparison of the incidence of developed infection between the two groups. The wound condition was monitored on a daily basis and radiographies were obtained at 12 weeks postoperatively. Infection-related laboratory markers (white blood cell count, erythrocyte sedimentation rate, and C-reactive protein values) were assessed at 3, 6, and 16 weeks postoperatively. Histologic analysis and cultures of tissue samples were also performed to evaluate the presence of infection. Results: Clinical and laboratory signs of infection were evident in 11 rabbits in Group A (36.7%), and 4 in Group B (13.3%). The difference between the groups was statistically significant (p = 0.04). Five animals in Group B (16.7%) had clinical and histologic signs of a foreign-body reaction with significantly elevated CRP and ESR values but no simultaneous presence of infection was identified (p = 0.04). Bone remodelling with thickening of the periosteum and surrounding sclerosis was demonstrated radiologically in animals developing infection or foreign-body reactions. Conclusions: Absorbable plates and screws show lower susceptibility to infection compared to titanium ones. However, their application is associated with foreign-body reaction and the potential need for a second surgical intervention.
Study on Mechanical Properties and Degradation Behavior of Magnesium Alloy Vascular Clip
Authors: Zhang Hongxu , Gao Ming , Tian Xiaoying , Cao Dali , Tan Lili
Published: October 2023

The Mg alloy vascular clip has biodegradability and good biocompatibility, which can improve the convenience and safety of clinical application. However, the Mg alloy vascular clip also has some disadvantages, such as an unreasonable structure design and a degradation rate which is too fast. In this study, the process of clamping blood vessels with a biodegradable Mg alloy (Mg-Zn-Nd-Zr and Mg-Zn-Nd) general V-type vascular clip was simulated by finite element simulation software (Abaqus). A new type of vascular clip, the P-type vascular clip, was analyzed and investigated through simulation. The differences between Mg alloy vascular clips of V-type and P-type were analyzed by finite element simulation. In addition, the effects of Zr element on the mechanical properties and corrosion resistance of P-type vascular clips were also investigated to improve the mechanical stability. The results show that during the V-type vascular clip closure of Mg-Zn-Nd-Zr alloy, this clip has some problems, such as uneven distribution of blood vessel stress, crevices in blood vessels and stress concentration. The improved P-type vascular clip has uniform closure, and there is no gap in the blood vessel, which can effectively avoid stress concentration. The improved P-type vascular clip is well closed and can effectively avoid stress concentration. The corrosion resistance of the Mg-Zn-Nd-Zr alloy P-type clip was better than that of the Mg-Zn-Nd alloy P-type clip (degradation rate of 2.02 mm/y and 2.61 mm/y on the 7th day, respectively). Mg-Zn-Nd-Zr alloy The P-type vascular clip remained closed even on the 7th day, which could meet the requirements of clinical application.
The Impact of Mechanical Debridement Techniques on Titanium Implant Surfaces: A Comparison of Sandblasted, Acid-Etched, and Femtosecond Laser-Treated Surfaces
Authors: Eun Seung-Mo , Son Keunbada , Hwang Sung-Min , Son Young-Tak , Kim Yong-Gun , Suh Jo-Young , Hwang Jun Ho , Kwon Sung-Min , Lee Jong Hoon , Kim Hyun Deok , Lee Kyu-Bok , Lee Jae-Mok
Published: October 2023

This study evaluated the effects of various mechanical debridement methods on the surface roughness (Ra) of dental implants, comparing femtosecond laser-treated surfaces with conventionally machined and sandblasted with large-grit sand and acid-etched (SLA) implant surfaces. The fabrication of grade 4 titanium (Ti) disks (10 mm in diameter and 1 mm thick) and the SLA process were carried out by a dental implant manufacturer (DENTIS; Daegu, Republic of Korea). Subsequently, disk surfaces were treated with various methods: machined, SLA, and femtosecond laser. Disks of each surface-treated group were post-treated with mechanical debridement methods: Ti curettes, ultrasonic scaler, and Ti brushes. Scanning electron microscopy, Ra, and wettability were evaluated. Statistical analysis was performed using the Kruskal–Wallis H test, with post-hoc analyses conducted using the Bonferroni correction (α = 0.05). In the control group, no significant difference in Ra was observed between the machined and SLA groups. However, femtosecond laser-treated surfaces exhibited higher Ra than SLA surfaces (p < 0.05). The application of Ti curette or brushing further accentuated the roughness of the femtosecond laser-treated surfaces, whereas scaling reduced the Ra in SLA surfaces. Femtosecond laser-treated implant surfaces, with their unique roughness and compositional attributes, are promising alternatives in dental implant surface treatments.
Rate of Complications after Hip Fractures Caused by Prolonged Time-to-Surgery Depends on the Patient’s Individual Type of Fracture and Its Treatment
Authors: Daginnus Alina , Schmitt Jan , Graw Jan Adriaan , Soost Christian , Burchard Rene
Published: October 2023

Introduction: Hip fractures are common injuries in the elderly and are usually treated with timely surgery. While severe postoperative complications are reported for up to 10% of patients, many studies identified predictive factors for the occurrence of complications postoperatively. A controversially discussed factor is “time-to-surgery”. The aim of the study was to examine if time-to-surgery was associated with the occurrence of complications and if the complication rate differed between the patient individual fracture types of intracapsular on the one hand and extracapsular hip fractures on the other hand. We hypothesized that time-to-surgery had less impact on complications in intracapsular hip fractures compared to extracapsular ones, and therefore, guidelines should pay attention to the patient individual case scenario. Materials and Methods: All patients who were admitted to the Department of Trauma and Orthopaedic Surgery of an academic teaching hospital for hip fracture surgery (n = 650) over a five-year period were included in the study. After the application of the exclusion criteria, such as periprosthetic or pathologic fractures, cases needed immediate surgical treatment, and after outlier adjustment, 629 cases remained in the study. Hip fractures were classified into intracapsular fractures (treated by hip arthroplasty) and extracapsular fractures (treated by intramedullary nailing osteosynthesis). The occurrence of severe complications in patients treated within 24 h was compared with patients treated later than 24 h after injury. For statistical evaluation, a multivariate logistic regression analysis was performed to investigate the impact of time-to-surgery interval on the occurrence of complications. Results: Patients with an extracapsular fracture, which was treated with intramedullary nailing (44.5%), rarely suffered a serious complication when surgery was performed within 24 h after injury. However, when the interval of the time-to-surgery was longer than 24 h, the complication rate increased significantly (8.63% vs. 25.0%, p = 0.002). In contrast to this finding in patients with intracapsular fractures (55.5%), which were treated with cemented arthroplasty, complication rates did not depend on the 24 h interval (26.17% vs. 20.83%, p = 0.567). Conclusions: The occurrence of complications after surgical treatment of hip fractures is associated with the time interval between injury and surgery. A 24 h time interval between injury and surgical procedure seems to play a major role only in extracapsular fractures treated with osteosynthesis but not in intracapsular fractures treated with arthroplasty. Therefore, guidelines should take notice of the patient individual case scenario and, in particular, the individual hip fracture type.
Influence of the COVID-19 Pandemic on Musculoskeletal Complaints and Psychological Well-Being of Employees in Public Services—A Cohort Study
Authors: Wolf Leonie , Maier Philipp , Deibert Peter , Schmal Hagen , Kubosch Eva Johanna
Published: October 2023

Background: The COVID-19 pandemic resulted in unprecedented restrictions on public and private life. The aim of the study was to investigate the impact of the COVID-19 pandemic on the physical and mental health of employees in the public sector, especially patient-related professions. Methods: For the data collection in summer 2021, an online questionnaire was used. Compared to a pre-pandemic point of time, the prevalence, frequency, and intensity of musculoskeletal pain, mental well-being, health status, and quality of life were recorded. Results: The questionnaire was completed by 1678 employees (f: 1045, m: 617). A total of 1504 employees (89.6%) were affected by complaints. Compared to before the pandemic, the prevalence and intensity of musculoskeletal complaints and psychological distress increased significantly. Patient-related professions (n = 204) showed significantly higher levels of stress and discomfort in several aspects (p < 0.05). Conclusions: Due to the COVID-19 pandemic, there was an increase in musculoskeletal complaints and a decrease in psychological well-being. Preventive factors related to mental health were identified as male gender, a middle- to older-age group, employees with children, and working from home. Attention should be drawn to these findings and prevention strategies should be brought into focus to strengthen the employees’ health. Special focus should be drawn to patient-related professions who are particularly confronted with pandemic-associated challenges.
Book Review: Ayres-Bennett and Fisher, Eds. (2022). Multilingualism and Identity: Interdisciplinary Perspectives. Cambridge: Cambridge University Press. ISBN: 978-1-108-49020-7 (hbk)
Authors: He Qi , Hu Ting
Published: October 2023

Against the backdrop of globalization, migration, and the growing dominance of English as a lingua franca, analyzing and understanding multilingualism and its relationship to identity is a complex and challenging task [...]
Building Agro-Industrial Capabilities in the Sugarcane Supply Chain in Brazil
Authors: Medina Gabriel da Silva , Costa Rommel Bernardes da
Published: October 2023

Background: This study aims to explore how domestic entrepreneurs can benefit from the thriving global agribusiness by establishing themselves in agro-industrial segments that can best remunerate capital and labour. The ways in which domestic entrepreneurs in Brazil enter different segments of the agribusiness industry were assessed with specific attention to implications for the development of local agro-industrial capabilities. Methods: We assessed the current market share of domestic companies in relation to foreign multinationals in various segments of the sugar and ethanol supply chain in Brazil. Results: Foreign multinationals are market leaders in the fertilizers, machinery and trading segments (domestic companies market share is 20.3%, 33.3% and 42.9% in those segments respectively). However, Brazilian companies have achieved higher market share in segments such as plant breeding, sugarcane processing and farming (domestic market share is 93.2%, 67.4% and 75.5% respectively). Plant breeding, farming and trading benefit from governmental support in research, subsidized credits and market policies respectively. Conclusions: By investing in agro-industrial sectors developing countries can benefit from agribusiness expansion for their economic growth. Investments in science and technology and domestic regulatory actions can help to build country capabilities, although the impacts are sometimes limited to the agro-industrial sectors where domestic companies are more competitive. These lessons can help other developing countries to assess their opportunities and challenges for agro-industrial development.