bluuebunny/crossref_metadata_2025 huggingface dataset:
Review of Macadamia Production in Malawi: Focusing on What, Where, How Much Is Produced and Major Constraints
Authors: Zuza Emmanuel Junior , Maseyk Kadmiel , Bhagwat Shonil , Emmott Andrew , Rawes Will , Araya Yoseph Negusse
Published: February 2021

Macadamia is an essential commodity crop in Malawi. The nuts are a lucrative commodity and are used for household consumption, income generation among farming families and as a foreign exchange earning crop at country-level. Macadamia production in Malawi has increased significantly in recent years. Malawi is the seventh top producer of macadamia nuts valued at £23.5 million, with a global market share of 3%. In 2018, the country was the fourth-largest exporter of macadamia nuts that were valued at £18.2 million. The majority (90%) of this crop was grown by large commercial estates with smallholder’s production only contributing about 10% of the total crop production. However, the smallholder sector is vital for the future growth of the macadamia sector in the country. Further, Malawian smallholders consider macadamia production as a low-input crop with large returns per unit area (£10.7 kg−1 ha−1), and it thus a lucrative commodity with high potential for poverty reduction and wealth creation among these farming families. This paper, therefore, explores: (i) the historical and current trends in macadamia nut production in Malawi; (ii) analyses the country’s macadamia value chain focusing on smallholder farmer contributions; and (iii) discusses the constraints of smallholder macadamia production in Malawi for informed policymaking. We conclude that the synthesis of the Malawian macadamia sub-sector provides an understanding of the vital contributions of macadamia to Malawi’s economic growth and improvement of livelihoods.
Gluten Protein Compositional Changes in Response to Nitrogen Application Rate
Authors: Horvat Daniela , Šimić Gordana , Dvojković Krešimir , Ivić Marko , Plavšin Ivana , Novoselović Dario
Published: February 2021

Field trials were conducted to evaluate the effect of nitrogen level on wheat protein content and composition in 16 cultivars over two years at three locations. The nitrogen treatment comprised two nitrogen levels, 0 kg ha−1 as low and 100 kg ha−1 as high nitrogen, applied as top dressings of 50 kg nitrogen per ha at tillering and stem extension growth stages. Increased nitrogen level generally enhanced grain protein by 11.3% (11.5% vs. 12.8%). Considering protein composition determined by reversed phase–high-pressure liquid chromatography, higher nitrogen supply generally enhanced the proportion of total gliadins, α-gliadins, γ-gliadins and high-molecular-weight glutenin subunits by 1.1%, 2.0%, 3.7%, 0.6% and 0.9%, respectively, and reduced albumins and globulins, ω-gliadins, total glutenins and low-molecular-weight glutenin subunits by 1.1%, 1.7%, 1.9% and 3.2%. Under a high nitrogen level, the historical cultivars Libellula, San Pastore and U-1 had a higher protein content (13.1–15.2%) with significantly higher total gliadins, which resulted in a significantly higher gliadin/glutenin ratio (1.68–1.92). In the modern cultivars, protein content varied between 11.4% and 14.6% with a well-balanced gliadin/glutenin ratio (1.08–1.50), except for cultivar MV Nemere which had a high gliadin/glutenin ratio at both nitrogen levels (1.81 vs. 1.87). In summary, increasing nitrogen level enhanced grain protein content while the composition of gliadin and glutenin fractions was changed to a lesser extent and was largely cultivar specific and therefore should be considered for wheat baking quality assessment and breeding purposes.
Pollen Paternity Can Affect Kernel Size and Nutritional Composition of Self-Incompatible and New Self-Compatible Almond Cultivars
Authors: Kämper Wiebke , Thorp Grant , Wirthensohn Michelle , Brooks Peter , Trueman Stephen J.
Published: February 2021

Breeding programs for horticultural tree crops focus on enhancing productivity, including developing tolerance to pests and diseases and improving crop quality. Pollination services are often critical for crop production, and pollen parents can affect crop quality. We often do not know which pollen parents produce highest quality offspring or, in self-compatible cultivars, how much of the crop comes from cross- versus self-pollination. We quantified the proportions of self- and cross-paternity in an open pollination setting of five standard commercial almond cultivars and of six new almond cultivars selected for yield, kernel size, taste or self-compatibility. We assessed how pollination by different parents affected kernel size and nutritional quality. Kernels from most commercial cultivars and from the new cultivars selected for taste and size resulted almost entirely from cross-pollination. Most kernels from the commercial cultivar ‘Price’ resulted from cross-pollination but 21% resulted from self-pollination. In contrast, 48–91% of kernels from the new self-compatible cultivars resulted from self-pollination. Different cross-pollen parents did not greatly affect kernel size or quality. The proportions of self-paternity in the new self-compatible cultivars varied strongly in an open pollination setting suggesting that some cultivars may be good candidates for establishing monovarietal orchards.
Effects of Modified Atmosphere Packaging and Chitosan Treatment on Quality and Sensorial Parameters of Minimally Processed cv. ‘Italia’ Table Grapes
Authors: Liguori Giorgia , Sortino Giuseppe , Gullo Gregorio , Inglese Paolo
Published: February 2021

Table grape is a non-climacteric fruit, very sensitive to water loss and gray mold during postharvest handling and storage. The aim of this work was to evaluate the effects of modified atmosphere packaging and chitosan treatment on quality and sensorial parameters of minimally processed cv. ‘Italia’ table grape during cold storage (14 days at 5 °C) and shelf-life (7 and 14 days of cold storage plus 5 days at 20 °C), reproducing a retail sales condition. Our data showed a significant effect of high CO2-modified atmosphere in combination with chitosan and alone on preserving quality, sensorial parameters, and delaying decay of minimally processed table grape. The most effective treatment in terms of preservation of quality, sensory, and nutritional quality was high-CO2 modified atmosphere packaging plus chitosan treatment. The overall results showed that when a proper modified atmosphere packaging is combined with a postharvest chitosan treatment, gray mold growth can effectively be controlled in ready-to-eat cv. ‘Italia’ table grape and that modified atmosphere packaging (MAP) with high levels of CO2 and reduced concentration of O2 in combination with chitosan treatment could prevent the negative effect of high-CO2 in-packages level, like berry browning and rachis browning and dehydration.
Expression of Apoptosis-Related Genes in Cat Testicular Tissue in Relation to Sperm Morphology and Seasonality—A Preliminary Study
Authors: Prochowska Sylwia , Partyka Agnieszka , Niżański Wojciech
Published: February 2021

Apoptosis is a crucial process in spermatogenesis, responsible for the elimination of abnormal sperm cells and testicular regression out of breeding season. The aim of this study was to assess if the expression of apoptosis-related genes in testicular tissue of domestic cats differed: (1) between normozoospermic and teratozoospermic donors, and (2) between reproductive and non-reproductive season. The expression of genes: BCL2L1, BCL2, BAX, BAD, FAS, FASLG, and caspases (CASP3, CASP8, CASP9, and CASP10) was analyzed by qRT-PCR in testicular tissue samples. During non-reproductive season significantly higher expression of two anti-apoptotic genes (BCL2L1 and BCL2) was observed. Additionally, there was a significant higher expression of CASP10 in teratozoospermic cats during non-reproductive than during reproductive season. No differences were noted between normozoospermic and teratozoospermic groups. Upregulation of some genes during the non-reproductive season indicates engagement of apoptotic mechanisms in the seasonal changes of semen quality in cats, however further studies on protein levels and analysis of changes on distinct testicular germinal layers are required. At the same time, teratozoospermia in the general population of cats seems to be not connected with dysregulation of apoptosis in the testes.
Overview of Native Chicken Breeds in Italy: Conservation Status and Rearing Systems in Use
Authors: Castillo Annelisse , Gariglio Marta , Franzoni Alessandro , Soglia Dominga , Sartore Stefano , Buccioni Arianna , Mannelli Federica , Cassandro Martino , Cendron Filippo , Castellini Cesare , Mancinelli Alice Cartoni , Iaffaldano Nicolaia , Iorio Michele Di , Marzoni Margherita , Salvucci Sonia , Cerolini Silvia , Zaniboni Luisa , Schiavone Achille
Published: February 2021

The most reared species of farm animal around the world is the chicken. However, the intensification of livestock systems has led to a gradual increase in the concentration of a limited number of breeds, resulting in substantial erosion to the genetic pool. The initial step of an ‘animal conservation program’ entails establishing the actual conservation statuses of the breeds concerned in a defined area; in this case, in Italy. To this end, a survey of breeds was performed by means of a census questionnaire divided into two parts. The first part collected information on breeds, breeders, housing facilities, and management aspects, the results of which are presented here. The second part of the questionnaire regarded chicken products and their markets, and these data will be reported in a second paper. The breed status of six chicken breeds was shown to be exceptionally worrying, with total numbers ranging from just 18 to 186 birds. Population sizes exceeding 1000 birds was identified for just four breeds, the maximum being 3400. Some improvements in status were noted in relation to breeds which had been the subject of conservation efforts in the past. The two most common breeds reported are the Bionda Piemontese, a double-purpose breed, and the Livorno egg-laying hen. Collo Nudo Italiano, Millefiori Piemontese, Pollo Trentino, and Tirolese chicken breeds and the Castano Precoce turkey breed were not listed by breeders at all. The most reported turkey breeds are the Bronzato Comune and the Ermellinato di Rovigo. The population sizes of native Italian poultry breeds were shown to be generally poor. Italian poultry farmers and the population at large are largely ignorant about indigenous poultry breeds. Thus, promoting the virtues of Italian breeds would help their conservation by encouraging breeders to rear these birds and consumers to buy their products. The identification of strategies to facilitate access to pure breed birds is essential, and will require the collaboration of university research centers, public entities, and breeders. The results presented in this paper constitute the initial part of a more complex conservation program.
A Set of Active Disturbance Rejection Controllers Based on Integrator Plus Dead-Time Models
Authors: Huba Mikulas , Moura Oliveira Paulo , Bistak Pavol , Vrancic Damir , Žáková Katarina
Published: February 2021

The paper develops and investigates a novel set of constrained-output robust controllers with selectable response smoothing degree designed for an integrator-plus-dead-time (IPDT) plant model. The input-output response of the IPDT system is internally approximated by several time-delayed, possibly higher-order plant models of increasing complexity. Since they all contain a single integrator, the presented approach can be considered as a generalization of active disturbance rejection control (ADRC). Due to the input/output model used, the controller commissioning can be based on a simplified process modeling, similar to the one proposed by Ziegler and Nichols. This allows it to be compared with several alternative controllers commonly used in practice. Its main advantage is simplicity, since it uses only two identified process parameters, even when dealing with more complex systems with distributed parameters. The proposed set of controllers with increasing complexity includes the stabilizing proportional (P), proportional-derivative (PD), or proportional-derivative-acceleration (PDA) controllers. These controllers can be complemented by extended state observers (ESO) for the reconstruction of all required state variables and non-measurable input disturbances, which also cover imperfections of a simplified plant modeling. A holistic performance evaluation on a laboratory heat transfer plant shows interesting results from the point of view of the optimal least sensitive solution with smooth input and output.
Influence of Cracking on the Durability of Reinforced Concrete with Carbon Nanotubes
Authors: Bogas Jose Alexandre , Ahmed Hawreen Hasan , Diniz Tomás
Published: February 2021

This study focuses on the influence of natural and artificially induced cracks on the durability of concrete reinforced with carbon nanotubes (CNT). Pre-cracked concrete mixes, unreinforced or reinforced with 0.1% CNT, are characterized in terms of capillary absorption, carbonation, and chloride penetration resistance, and compared to the uncracked reference concrete. The mechanical strength and durability properties were improved in uncracked CNT-reinforced concrete, without significantly affecting its density and workability. The efficiency of CNT was higher when the concrete was previously subjected to drying conditions. For all tested properties, the incorporation of CNT was effective in reducing the influence of artificial and natural cracks on concrete durability. The main contribution of CNT occurred in the crack surrounding region. Depending on the analyzed property and cracking conditions, the significant reduction of durability in cracked concrete may be 10–30% attenuated when CNT is incorporated. The effect was more pronounced in mechanically induced natural cracks, where CNT may better participate in their vicinity.
Hypervelocity Impacts on Satellite Sandwich Structures—A Review of Experimental Findings and Predictive Models
Authors: Carriere Riley , Cherniaev Aleksandr
Published: February 2021

Sandwich panels are widely used in the design of unmanned satellites and, in addition to having a structural function, can often serve as shielding, protecting the satellites’ equipment from hypervelocity impacts (HVI) of orbital debris and micrometeoroids. This paper provides a comprehensive review of experimental studies in the field of HVI on sandwich panels with honeycomb- and open-cell foam cores, as well as an examination of available predictive models for the assessment of the panels’ ballistic limits. The emphasis of the review is placed on: (i) identifying gaps in the existing experimental database and the appropriate directions for its further expansion; and (ii) understanding the limitations of the available predictive models and the potential for their improvement.
Male Sexual Preference for Female Swimming Activity in the Guppy (Poecilia reticulata)
Authors: Bierbach David , Wenchel Ronja , Gehrig Stefan , Wersing Serafina , O’Connor Olivia L. , Krause Jens
Published: February 2021

Mate choice that is based on behavioural traits is a common feature in the animal kingdom. Using the Trinidadian guppy, a species with mutual mate choice, we investigated whether males use female swimming activity—a behavioural trait known to differ consistently among individuals in many species—as a trait relevant for their mate choice. In the first experiment, we assessed male and female activity in an open field test alone (two repeated measures) and afterwards in heterosexual pairs (two repeated measures). In these pairs, we simultaneously assessed males’ mating efforts by counting the number of sexual behaviours (courtship displays and copulations). Male and female guppies showed consistent individual differences in their swimming activity when tested both alone and in a pair, and these differences were maintained across both test situations. When controlling for male swimming behaviour and both male and female body size, males performed more courtship displays towards females with higher swimming activity. In a second experiment, we tested for a directional male preference for swimming activity by presenting males video animations of low- and high-active females in a dichotomous choice test. In congruence with experiment 1, we found males to spend significantly more time in association with the high-active female stimulus. Both experiments thus point towards a directional male preference for higher activity levels in females. We discuss the adaptive significance of this preference as activity patterns might indicate individual female quality, health or reproductive state while, mechanistically, females that are more active might be more detectable to males as well.
Bliss in and Out of the Body: The (Extra)Corporeal Space Is Impervious to Social Pleasant Touch
Authors: Spaccasassi Chiara , Frigione Ivana , Maravita Angelo
Published: February 2021

Slow, gentle stimulation of hairy skin is generally accompanied by hedonic sensations. This phenomenon, also known as (positive) affective touch, is likely to be the basis of affiliative interactions with conspecifics by promoting inter-individual bindings. Previous studies on healthy humans have demonstrated that affective touch can remarkably impact behavior. For instance, by administering the Rubber Hand Illusion (RHI) paradigm, the embodiment of a fake hand enhances after a slow, affective touch compared to a fast, neutral touch. However, results coming from this area are not univocal. In addition, there are no clues in the existing literature on the relationship between affective touch and the space around our body. To overcome these lacks, we carried out two separate experiments where participants underwent a RHI paradigm (Experiment 1) and a Visuo-Tactile Interaction task (Experiment 2), designed to tap into body representation and peripersonal space processing, respectively. In both experiments, an affective touch (CT-optimal, 3 cm/s) and neutral touch (CT-suboptimal, 18 cm/s) were delivered by the experimenter on the dorsal side of participants’ hand through a “skin to skin” contact. In Experiment 1, we did not find any modulation of body representation—not at behavioral nor at a physiological level—by affective touch. In Experiment 2, no visuo-tactile spatial modulation emerged depending upon the pleasantness of the touch received. These null findings are interpreted in the light of the current scientific context where the real nature of affective touch is often misguided, and they offer the possibility to pave the way for understanding the real effects of affective touch on body/space representation.
Antigenic Essence: Upgrade of Cellular Cancer Vaccines
Authors: Lokhov Petr G. , Balashova Elena E.
Published: February 2021

The development of anticancer immunotherapy is characterized by several approaches, the most recognized of which include cellular vaccines, tumor-associated antigens (TAAs), neoantigens, and chimeric antigen receptor T cells (CAR-T). This paper presents antigenic essence technology as an effective means for the production of new antigen compositions for anticancer vaccination. This technology is developed via proteomics, cell culture technology, and immunological assays. In terms of vaccine development, it does not fit into any of the above-noted approaches and can be considered a new direction. Here we review the development of this technology, its main characteristics, comparison with existing approaches, and the features that distinguish it as a novel approach to anticancer vaccination. This review will also highlight the benefits of this technology over other approaches, such as the ability to control composition, optimize immunogenicity and similarity to target cells, and evade major histocompatibility complex restriction. The first antigenic essence products, presented under the SANTAVAC brand, are also described.
Biology of NSCLC: Interplay between Cancer Cells, Radiation and Tumor Immune Microenvironment
Authors: Tubin Slavisa , Khan Mohammad K. , Gupta Seema , Jeremic Branislav
Published: February 2021

The overall prognosis and survival of non-small cell lung cancer (NSCLC) patients remain poor. The immune system plays an integral role in driving tumor control, tumor progression, and overall survival of NSCLC patients. While the tumor cells possess many ways to escape the immune system, conventional radiotherapy (RT) approaches, which are directly cytotoxic to tumors, can further add additional immune suppression to the tumor microenvironment by destroying many of the lymphocytes that circulate within the irradiated tumor environment. Thus, the current immunogenic balance, determined by the tumor- and radiation-inhibitory effects is significantly shifted towards immunosuppression, leading to poor clinical outcomes. However, newer emerging evidence suggests that tumor immunosuppression is an “elastic process” that can be manipulated and converted back into an immunostimulant environment that can actually improve patient outcome. In this review we will discuss the natural immunosuppressive effects of NSCLC cells and conventional RT approaches, and then shift the focus on immunomodulation through novel, emerging immuno- and RT approaches that promise to generate immunostimulatory effects to enhance tumor control and patient outcome. We further describe some of the mechanisms by which these newer approaches are thought to be working and set the stage for future trials and additional preclinical work.
Lessons Learned from Targeting IGF-I Receptor in Thyroid-Associated Ophthalmopathy
Authors: Janssen Joseph A.M.J.L. , Smith Terry J.
Published: February 2021

Complex immunological mechanisms underlie the pathogenesis of thyroid-associated ophthalmopathy (TAO). Historical models of Graves’ disease and TAO have focused almost entirely on autoimmune reactivity directed against the thyrotropin receptor (TSHR). The insulin-like growth factor-I receptor (IGF-IR) has been proposed as a second participating antigen in TAO by virtue of its interactions with IGFs and anti-IGF-IR antibodies generated in Graves’ disease. Furthermore, the IGF-IR forms with TSHR a physical and functional complex which is involved in signaling downstream from both receptors. Inhibition of IGF-IR activity results in attenuation of signaling initiated at either receptor. Based on the aggregate of findings implicating IGF-IR in TAO, the receptor has become an attractive therapeutic target. Recently, teprotumumab, a human monoclonal antibody IGF-IR inhibitor was evaluated in two clinical trials of patients with moderate to severe, active TAO. Those studies revealed that teprotumumab was safe and highly effective in reducing disease activity and severity. Targeting IGF-IR with specific biologic agents may result in a paradigm shift in the therapy of TAO.
Interstitial Annelida
Authors: Worsaae Katrine , Kerbl Alexandra , Domenico Maikon Di , Gonzalez Brett C. , Bekkouche Nicolas , Martínez Alejandro
Published: February 2021

Members of the following marine annelid families are found almost exclusively in the interstitial environment and are highly adapted to move between sand grains, relying mostly on ciliary locomotion: Apharyngtidae n. fam., Dinophilidae, Diurodrilidae, Nerillidae, Lobatocerebridae, Parergodrilidae, Polygordiidae, Protodrilidae, Protodriloididae, Psammodrilidae and Saccocirridae. This article provides a review of the evolution, systematics, and diversity of these families, with the exception of Parergodrilidae, which was detailed in the review of Orbiniida by Meca, Zhadan, and Struck within this Special Issue. While several of the discussed families have previously only been known by a few described species, recent surveys inclusive of molecular approaches have increased the number of species, showing that all of the aforementioned families exhibit a high degree of cryptic diversity shadowed by a limited number of recognizable morphological traits. This is a challenge for studies of the evolution, taxonomy, and diversity of interstitial families as well as for their identification and incorporation into ecological surveys. By compiling a comprehensive and updated review on these interstitial families, we hope to promote new studies on their intriguing evolutionary histories, adapted life forms and high and hidden diversity.
On the Adoption of Radiomics and Formal Methods for COVID-19 Coronavirus Diagnosis
Authors: Santone Antonella , Belfiore Maria Paola , Mercaldo Francesco , Varriano Giulia , Brunese Luca
Published: February 2021

Considering the current pandemic, caused by the spreading of the novel Coronavirus disease, there is the urgent need for methods to quickly and automatically diagnose infection. To assist pathologists and radiologists in the detection of the novel coronavirus, in this paper we propose a two-tiered method, based on formal methods (to the best of authors knowledge never previously introduced in this context), aimed to (i) detect whether the patient lungs are healthy or present a generic pulmonary infection; (ii) in the case of the previous tier, a generic pulmonary disease is detected to identify whether the patient under analysis is affected by the novel Coronavirus disease. The proposed approach relies on the extraction of radiomic features from medical images and on the generation of a formal model that can be automatically checked using the model checking technique. We perform an experimental analysis using a set of computed tomography medical images obtained by the authors, achieving an accuracy of higher than 81% in disease detection.
Next-Generation Molecular Investigations in Lysosomal Diseases: Clinical Integration of a Comprehensive Targeted Panel
Authors: Sudrié-Arnaud Bénédicte , Snanoudj Sarah , Dabaj Ivana , Dranguet Hélène , Abily-Donval Lenaig , Lebas Axel , Vezain Myriam , Héron Bénédicte , Marie Isabelle , Duval-Arnould Marc , Marret Stéphane , Tebani Abdellah , Bekri Soumeya
Published: February 2021

Diagnosis of lysosomal disorders (LDs) may be hampered by their clinical heterogeneity, phenotypic overlap, and variable age at onset. Conventional biological diagnostic procedures are based on a series of sequential investigations and require multiple sampling. Early diagnosis may allow for timely treatment and prevent clinical complications. In order to improve LDs diagnosis, we developed a capture-based next generation sequencing (NGS) panel allowing the detection of single nucleotide variants (SNVs), small insertions and deletions, and copy number variants (CNVs) in 51 genes related to LDs. The design of the LD panel covered at least coding regions, promoter region, and flanking intronic sequences for 51 genes. The validation of this panel consisted in testing 21 well-characterized samples and evaluating analytical and diagnostic performance metrics. Bioinformatics pipelines have been validated for SNVs, indels and CNVs. The clinical output of this panel was tested in five novel cases. This capture-based NGS panel provides an average coverage depth of 474× which allows the detection of SNVs and CNVs in one comprehensive assay. All the targeted regions were covered above the minimum required depth of 30×. To illustrate the clinical utility, five novel cases have been sequenced using this panel and the identified variants have been confirmed using Sanger sequencing or quantitative multiplex PCR of short fluorescent fragments (QMPSF). The application of NGS as first-line approach to analyze suspected LD cases may speed up the identification of alterations in LD-associated genes. NGS approaches combined with bioinformatics analyses, are a useful and cost-effective tool for identifying the causative variations in LDs.
Detection of Autophagy-Related Gene Expression by Conjunctival Impression Cytology in Age-Related Macular Degeneration
Authors: Shu Chih-Wen , Bee Youn-Shen , Chen Jiunn-Liang , Tsen Chui-Lien , Tsai Wei-Lun , Sheu Shwu-Jiuan
Published: February 2021

Purpose: To investigate the association of autophagy-related gene expression with age-related macular degeneration (AMD). Methods: Patients with AMD were recruited for analysis by conjunctival impression cytology. mRNA was assessed by real-time polymerase chain reaction (RT-PCR) to evaluate whether the expression of 26 autophagy-related genes (ATGs) was correlated with AMD. Further studies on cell viability and autophagic flux in response to oxidative stress by H2O2 were performed in human retinal pigment epithelial (RPE) cell lines based on the results of impression cytology. Results: Both the neovascular AMD (nAMD) and polypoidal choroidal vasculopathy (PCV) groups had significantly higher mRNA levels of gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) and microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) than the control group, but there was no significant difference between these two groups. Age difference existed only in the AMD group. GABARAPL1 and MAP1LC3B mRNA expression increased significantly after acute oxidative stress in adult retinal pigment epithelial (ARPE-19) cells. Cell viability significantly increased and decreased in the cells harboring GABARAPL1 expression vector and silenced with siRNA against GABARAPL1, respectively, during short-term oxidative stress, whereas viability increased in the GABARAPL1-silenced cells after long-term oxidative stress. Silencing GABARAPL1 itself caused a reduction in autophagic flux under both short and long-term oxidative stress. Conclusion: Our study showed the possibility of assessing autophagy-related gene expression by conjunctival impression cytology. GABARAPL1 was significantly higher in AMD. Although an in vitro study showed an initial protective effect of autophagy, a cell viability study revealed the possibility of a harmful effect after long-term oxidative injury. The underlying mechanism or critical factors require further investigation.
Non-Invertible Public Key Certificates
Authors: Lizama-Perez Luis Adrián , López R. J. Mauricio
Published: February 2021

Post-quantum public cryptosystems introduced so far do not define a scalable public key infrastructure for the quantum era. We demonstrate here a public certification system based on Lizama’s non-invertible key exchange protocol which can be used to implement a secure, scalable, interoperable and efficient public key infrastructure (PKI). We show functionality of certificates across different certification domains. Finally, we discuss a method that enables non-invertible certificates to exhibit perfect forward secrecy (PFS).
Risk Analysis Using Failure Modes, Effects, and Criticality Analysis for Transmission Network Assets
Authors: Suwanasri Cattareeya , Saribut Surapol , Suwanasri Thanapong , Phadungthin Rattanakorn
Published: February 2021

In this paper, we apply the Failure Modes, Effects, and Criticality Analysis (FMECA) approach to the criticality and risk analysis of the efficiency, safety/reliability, environment, and financial criteria of the high voltage transmission line. In the efficiency analysis, the weighting-scoring method and analytical hierarchy process are applied to obtain the line renovation index, which is interpreted as efficiency severity. The safety/reliability severity relates to the system impacts are caused by failure of the equipment and the system. The environmental severity takes social impacts and pollution into consideration. The financial severity involves the cost of spare parts and maintenance costs, which are interpreted as the financial severity of the lines. Twenty practical transmission lines in Thailand with actual data for 115, 230, and 500 kV were studied. The transmission line components are divided into eight components including the conductor, conductor accessories, insulator, steel structure, foundation, lightning protection system, tower accessories, and right-of-way. The severity and criticality of the components and transmission lines are analyzed and plotted in criticality matrices based on four criteria. This criticality matrix is presented at four different levels including very low, low, medium, and high risk, as represented by four color bands: green, yellow, orange, and red, respectively. The results show that three 115 kV lines are the first priority for short-term reconstruction planning because of their unacceptable condition and high risk in terms of safety/reliability and financing. Recommendations for maintenance strategy and risk mitigation are proposed for the utility according to the utility’s actual criticality. Ultimately, maintenance planning of the transmission system can be effectively managed with higher reliability, a lower risk of failure, and a lower cost of maintenance.
Impact of the Lockdown during the COVID-19 Pandemic on Electricity Use by Residential Users
Authors: Bielecki Sławomir , Skoczkowski Tadeusz , Sobczak Lidia , Buchoski Janusz , Maciąg Łukasz , Dukat Piotr
Published: February 2021

The COVID-19 pandemic and the associated lockdown can be regarded as a forced social experiment, the results of which show how to use energy under specific conditions. During this period, there was a reduction in electricity consumption at the level of the power system, but a different specificity distinguishes the group of household users. The article aims at presenting and analysing the identified issues concerning residential electricity users based on the experience from the COVID-19 pandemic lockdown. Data from energy meters from almost 7000 flats in Warsaw’s housing estates during the lockdown in 2020 and the analogous period before the pandemic were used. The analysis showed that, on average, residential users staying practically the whole day in their flats increased their energy consumption, but without increasing their average daily peak power, smoothing the profile in the morning hours to the level reaching the peak power that had occurred in the analogous period before the lockdown. The peak power of the sections feeding the different numbers of dwellings also remained practically unchanged during the lockdown compared to the pre-pandemic period. The pressure to work and educate remotely should contribute to an increase in the digital competence of society, which may result in an increased interest in new forms of activity and cooperation based on demand-side response and prosumption mechanisms, with digital settlements for energy exchange and services.
Root Foraging Ability for Phosphorus in Different Genotypes Taxodium ‘Zhongshanshan’ and Their Parents under Phosphorus Deficiency
Authors: Xie Rongxiu , Hua Jianfeng , Yin Yunlong , Wan Fuxu
Published: February 2021

The phosphorus (P) deficiency is the one of the key constraints for Taxodium ‘Zhongshanshan’ afforestation. A hydroponic experiment was conducted to explore root foraging ability for P in different genotypes of Taxodium ‘Zhongshanshan’ (T.‘Zhongshanshan’) and their parents (T.mucronatum and T.distichum). Five P levels of CK (31 mg/L), P15 (15 mg/L), P10 (10 mg/L), P5 (5 mg/L), and P0 (0 mg/L) were set up as the P deficiency stress treatment. The plant P contents, root morphological indices, and plant growth traits of different taxodium genotypes were measured. Meanwhile, the root foraging ability for P was evaluated with the membership function method in combination with weight. Results showed that: (1) Except the plant P content, the root morphology, plant net biomass, and height showed significant differences among the different genotypes (p < 0.05); the P deficiency stress had no significant influence on root morphology, but a significant influence on plant net biomass and height and P content; (2) T.mucronatum and T.‘Zhongshanshan’302 had relatively lower values of root length, root surface area, root volume, and plant net biomass, but had no difference of plant P content with the other genotypes; (3) T.mucronatum and T.‘Zhongshanshan’302 had higher root foraging ability for P than the other genotypes; (4) the stepwise regression analysis revealed the root volume as the main factor significantly influencing the root foraging ability. This study concluded that different genotypes of T.’Zhongshanshan’ and their parents had different root foraging ability for P, and breeding and screening the fine varieties is conducive for the afforestation in P-limited areas.
Development and Evaluation of an Online Education-Entertainment Intervention to Increase Knowledge of HIV and Uptake of HIV Testing among Colombian Men Who Have Sex with Men (MSM)
Authors: del Río-González Ana María , Zea Maria Cecilia , Calabrese Sarah K. , Betancourt Fabián , Pacheco-Cabrales Jorge , Estrada-Santiago Yacid , Poppen Paul J.
Published: February 2021

Using a community-based participatory approach, we developed a film to promote HIV testing among young men who have sex with men (MSM) in Bogotá. Using a 5-step process to develop the intervention, we conducted 11 focus groups with MSM (n = 141) to receive community feedback at each step. To evaluate the intervention we recruited 300 young MSM to complete a baseline survey in December 2017. Between February–June 2018, 63 participants watched the film and completed a post-viewing survey, which showed the intervention was acceptable for the target population. Between August–December 2018, 48 MSM who watched the film and 47 who did not (control group) completed a follow-up survey. To obtain preliminary evidence of the efficacy of the intervention, we assessed the main effect of time (baseline vs. follow-up) and the interaction between time and group (intervention vs. control) on HIV testing uptake and intentions, and knowledge of HIV transmission dynamics and HIV-related rights. Knowledge of HIV rights increased from baseline to follow-up in the intervention group only. HIV Knowledge increased for both groups. HIV testing intentions increased significantly more for non-gay-identified men in the intervention group, but the overall effect of the intervention was not significant. Testing uptake did not change.
Aquaporins in Immune Cells and Inflammation: New Targets for Drug Development
Authors: da Silva Inês V. , Soveral Graça
Published: February 2021

The mammalian immune system senses foreign antigens by mechanisms that involve the interplay of various kinds of immune cells, culminating in inflammation resolution and tissue clearance. The ability of the immune cells to communicate (via chemokines) and to shift shape for migration, phagocytosis or antigen uptake is mainly supported by critical proteins such as aquaporins (AQPs) that regulate water fluid homeostasis and volume changes. AQPs are protein channels that facilitate water and small uncharged molecules’ (such as glycerol or hydrogen peroxide) diffusion through membranes. A number of AQP isoforms were found upregulated in inflammatory conditions and are considered essential for the migration and survival of immune cells. The present review updates information on AQPs’ involvement in immunity and inflammatory processes, highlighting their role as crucial players and promising targets for drug discovery.
How to Select Firefly Luciferin Analogues for In Vivo Imaging
Authors: Saito-Moriya Ryohei , Nakayama Jun , Kamiya Genta , Kitada Nobuo , Obata Rika , Maki Shojiro A. , Aoyama Hiroshi
Published: February 2021

Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.
Insulin-Like Growth Factor 2 As a Possible Neuroprotective Agent and Memory Enhancer—Its Comparative Expression, Processing and Signaling in Mammalian CNS
Authors: Beletskiy Alexander , Chesnokova Ekaterina , Bal Natalia
Published: February 2021

A number of studies performed on rodents suggest that insulin-like growth factor 2 (IGF-2) or its analogs may possibly be used for treating some conditions like Alzheimer’s disease, Huntington’s disease, autistic spectrum disorders or aging-related cognitive impairment. Still, for translational research a comparative knowledge about the function of IGF-2 and related molecules in model organisms (rats and mice) and humans is necessary. There is a number of important differences in IGF-2 signaling between species. In the present review we emphasize species-specific patterns of IGF-2 expression in rodents, humans and some other mammals, using, among other sources, publicly available transcriptomic data. We provide a detailed description of Igf2 mRNA expression regulation and pre-pro-IGF-2 protein processing in different species. We also summarize the function of IGF-binding proteins. We describe three different receptors able to bind IGF-2 and discuss the role of IGF-2 signaling in learning and memory, as well as in neuroprotection. We hope that comprehensive understanding of similarities and differences in IGF-2 signaling between model organisms and humans will be useful for development of more effective medicines targeting IGF-2 receptors.
TFF1 Induces Aggregation and Reduces Motility of Helicobacter pylori
Authors: Eletto Daniela , Vllahu Megi , Mentucci Fatima , Del Gaudio Pasquale , Petrella Antonello , Porta Amalia , Tosco Alessandra
Published: February 2021

Gastric cancer is considered one of the most common malignancies in humans and Helicobacter pylori infection is the major environmental risk factor of gastric cancer development. Given the high spread of this bacterium whose infection is mostly asymptomatic, H. pylori colonization persists for a long time, becoming chronic and predisposing to malignant transformation. The first defensive barrier from bacterial infection is constituted by the gastric mucosa that secretes several protective factors, among which is the trefoil factor 1 (TFF1), that, as mucin 5AC, binds the bacterium. Even if the protective role of TFF1 is well-documented, the molecular mechanisms that confer a beneficial function to the interaction among TFF1 and H. pylori remain still unclear. Here we analyze the effects of this interaction on H. pylori at morphological and molecular levels by means of microscopic observation, chemiotaxis and motility assays and real-time PCR analysis. Our results show that TFF1 favors aggregation of H. pylori and significantly slows down the motility of the bacterium across the mucus. Such aggregates significantly reduce both flgE and flaB gene transcription compared with bacteria not incubated with TFF1. Finally, our results suggest that the interaction between TFF1 and the bacterium may explain the frequent persistence of H. pylori in the human host without inducing disease.
The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Anchors the Peptidoglycan Layer by Spanning It with All Subunits
Authors: Consoli Elisa , Collet Jean-François , den Blaauwen Tanneke
Published: February 2021

Gram-negative bacteria possess a three-layered envelope composed of an inner membrane, surrounded by a peptidoglycan (PG) layer, enclosed by an outer membrane. The envelope ensures protection against diverse hostile milieus and offers an effective barrier against antibiotics. The layers are connected to each other through many protein interactions. Bacteria evolved sophisticated machineries that maintain the integrity and the functionality of each layer. The β-barrel assembly machinery (BAM), for example, is responsible for the insertion of the outer membrane integral proteins including the lipopolysaccharide transport machinery protein LptD. Labelling bacterial cells with BAM-specific fluorescent antibodies revealed the spatial arrangement between the machinery and the PG layer. The antibody detection of each BAM subunit required the enzymatic digestion of the PG layer. Enhancing the spacing between the outer membrane and PG does not abolish this prerequisite. This suggests that BAM locally sets the distance between OM and the PG layer. Our results shed new light on the local organization of the envelope.
The Alter Retina: Alternative Splicing of Retinal Genes in Health and Disease
Authors: Aísa-Marín Izarbe , García-Arroyo Rocío , Mirra Serena , Marfany Gemma
Published: February 2021

Alternative splicing of mRNA is an essential mechanism to regulate and increase the diversity of the transcriptome and proteome. Alternative splicing frequently occurs in a tissue- or time-specific manner, contributing to differential gene expression between cell types during development. Neural tissues present extremely complex splicing programs and display the highest number of alternative splicing events. As an extension of the central nervous system, the retina constitutes an excellent system to illustrate the high diversity of neural transcripts. The retina expresses retinal specific splicing factors and produces a large number of alternative transcripts, including exclusive tissue-specific exons, which require an exquisite regulation. In fact, a current challenge in the genetic diagnosis of inherited retinal diseases stems from the lack of information regarding alternative splicing of retinal genes, as a considerable percentage of mutations alter splicing or the relative production of alternative transcripts. Modulation of alternative splicing in the retina is also instrumental in the design of novel therapeutic approaches for retinal dystrophies, since it enables precision medicine for specific mutations.
HPV Infection in Middle Ear Squamous Cell Carcinoma: Prevalence, Genotyping and Prognostic Impact
Authors: Cristalli Giovanni , Venuti Aldo , Giudici Fabiola , Paolini Francesca , Ferreli Fabio , Mercante Giuseppe , Spriano Giuseppe , Boscolo Nata Francesca
Published: February 2021

Middle ear squamous cell carcinoma (MESCC) is rare. Human Papilloma Virus (HPV) infection has been found in a significant number of cases of MESCC. Despite the emerging role of HPV in oncogenesis, its role in the pathogenesis and prognosis of MESCC is not known. This study aims to identify the prognostic impact of alpha and beta HPV in MESCC and its correlation with p16 protein. We retrospectively investigated 33 patients with MESCC surgically treated between 2004 and 2016. HPV DNA was ascertained by polymerase chain reaction (PCR) and P16INK4a detection was performed. Disease-specific survival (DSS) and cumulative incidence of recurrence were calculated in relation to HPV presence and genotype. p16 sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) in predicting HPV infection were calculated. HPV was detected in 66.7% of patients (36.4% alpha HPV, 63.6% beta HPV). Five-year DSS was 55.0% and was not statistically related to HPV presence (p = 0.55) or genotype (p = 0.87). Five-year cumulative incidence of recurrence was 46 %, and was not statistically related to HPV presence (p = 0.22) or genotype (p = 0.44). p16 sensitivity, specificity, PPV, and NPV in predicting HPV infection were 27.3%, 36.4%, 46.2%, and 20.0%, respectively. In our experience, beta HPV was more frequent than alpha HPV in MESCC. Neither HPV presence nor HPV genotypes relate to DSS or cumulative incidence of recurrence. p16 expression was not predictive for HPV infection in MESCC. The role of HPV infection in oncogenesis, maintenance, and prognosis of MESCC seems to be different from that in oropharynx and skin cancer.