bluuebunny/crossref_metadata_2025 huggingface dataset:
Involvement in Physical Fights among School Attending Adolescents: A Nationally Representative Sample from Kuwait
Authors: Shaikh Masood Ali , Abio Anne P. , Adedimeji Adebola A. , Lowery Wilson Michael
Published: January 2020

Background: Interpersonal violence in school settings is an important public health problem worldwide. This study investigated the individual and social correlates for being involved in a physical fight amongst a nationally representative sample of school-attending adolescents in Kuwait. Methods: We carried out bivariate and multivariate analyses to determine the strength and direction of associations with adolescent involvement in problematic fighting behavior within a 12-month recall period. Results: Within a total sample of 3637, n = 877 (25.2%) of school-attending adolescents reported being involved in two or more physical fights during the recall period. The multivariate analysis indicated that being male (OR = 2.71; CI = 1.88–3.90), a victim of bullying (OR = 2.77; CI = 2.14–3.58), truancy (OR = 2.52; CI = 1.91–3.32), planning a suicide (OR = 2.04; CI = 1.49–2.78) and food deprivation (OR = 1.91; CI = 1.37–2.65) were associated with an increased risk of involvement in physical fighting. Peer support in the form of having close friends (OR = 0.85; CI = 0.76–0.96) was found to be associated with a reduced involvement in fighting behavior. Conclusion: The results, when taken together, suggest that supportive school environments may represent important settings for violence mitigation and prevention strategies.
Epigenetic Silencing of Ubiquitin Specific Protease 4 by Snail1 Contributes to Macrophage-Dependent Inflammation and Therapeutic Resistance in Lung Cancer
Authors: Lai Chao-Yang , Yeh Da-Wei , Lu Chih-Hao , Liu Yi-Ling , Chuang Yu-Chen , Ruan Jhen-Wei , Kao Cheng-Yuan , Huang Li-Rung , Chuang Tsung-Hsien
Published: January 2020

There is a positive feedback loop driving tumorigenesis and tumor growth through coordinated regulation of epigenetics, inflammation, and stemness. Nevertheless, the molecular mechanism linking these processes is not well understood. In this study, we analyzed the correlation of de-ubiquitinases (DUBs) expression with survival data from the OncoLnc database. Among the DUBs analyzed, ubiquitin specific protease 4 (USP4) had the lowest negative Cox coefficient. Low expression of USP4 was associated with poor survival among lung cancer patients and was inversely correlated with expression of stemness and inflammation markers. Expression of USP4 were reduced at more advanced stages of lung cancer. Mechanistically, expression of USP4 was downregulated in snail1-overexpressing and stemness-enriched lung cancer cells. Snail1 was induced in lung cancer cells by interaction with macrophages, and epigenetically suppressed USP4 expression by promoter methylation. Stable knockdown of USP4 in lung cancer cells enhanced inflammatory responses, stemness properties, chemotherapy resistance, and the expression of molecules allowing escape from immunosurveillance. Further, mice injected with USP4 knockdown lung cancer cells demonstrated enhanced tumorigenesis and tumor growth. These results reveal that the Snail1-mediated suppression of USP4 is a potential mechanism to orchestrate epigenetic regulation, inflammation and stemness for macrophage-promoted tumor progression.
TYK2 in Tumor Immunosurveillance
Authors: Karjalainen Anzhelika , Shoebridge Stephen , Krunic Milica , Simonović Natalija , Tebb Graham , Macho-Maschler Sabine , Strobl Birgit , Müller Mathias
Published: January 2020

We review the history of the tyrosine kinase 2 (TYK2) as the founding member of the Janus kinase (JAK) family and outline its structure-function relation. Gene-targeted mice and hereditary defects of TYK2 in men have established the biological and pathological functions of TYK2 in innate and adaptive immune responses to infection and cancer and in (auto-)inflammation. We describe the architecture of the main cytokine receptor families associated with TYK2, which activate signal transducers and activators of transcription (STATs). We summarize the cytokine receptor activities with well characterized dependency on TYK2, the types of cells that respond to cytokines and TYK2 signaling-induced cytokine production. TYK2 may drive beneficial or detrimental activities, which we explain based on the concepts of tumor immunoediting and the cancer-immunity cycle in the tumor microenvironment. Finally, we summarize current knowledge of TYK2 functions in mouse models of tumor surveillance. The biology and biochemistry of JAKs, TYK2-dependent cytokines and cytokine signaling in tumor surveillance are well covered in recent reviews and the oncogenic properties of TYK2 are reviewed in the recent Special Issue ‘Targeting STAT3 and STAT5 in Cancer’ of Cancers.
Prediction of Postoperative Clinical Outcomes in Resected Stage I Non-Small Cell Lung Cancer Focusing on the Preoperative Glasgow Prognostic Score
Authors: Lindenmann Joerg , Fink-Neuboeck Nicole , Taucher Valentin , Pichler Martin , Posch Florian , Brcic Luka , Smolle Elisabeth , Koter Stephan , Smolle Josef , Smolle-Juettner Freyja Maria
Published: January 2020

Background: The Glasgow Prognostic Score (GPS), which consists of albumin and C-reactive protein (CRP), may predict overall survival (OS) in cancer patients. The aim of this retrospective analysis was to evaluate the clinical impact of the preoperative GPS on patients with resected early stage non-small cell lung cancer (NSCLC). Methods: 300 patients with curatively resected stage I NSCLC were followed-up for OS, recurrence-free survival (RFS), cancer-specific survival (CSS), and death from other causes. Results: 229 patients (76%) had a preoperative GPS of 0, and 71 (24%) a GPS ≥ 1. The three-year probabilities of RFS, OS, CSS, and death from other causes were 81%, 84%, 88%, and 96% in patients with GPS = 0, and 79%, 74%, 91%, and 82% in patients with a GPS ≥ 1, respectively. GPS ≥ 1 was significantly associated with a higher risk of death from other causes (p = 0.022), serving as an independent predictor of death from other causes (p = 0.034). Pathologically elevated CRP levels (CRP > 5 mg/L) were found in 91 patients (30%). The mean CRP level was 7.88 ± 15.80 mg/L (0.5–135.6 mg/L). Pre-treatment CRP level was significantly associated with coronary heart disease (p < 0.0001), histology (p = 0.013), tumor size (p = 0.018), tumor stage (p = 0.002), and vascular invasion (p = 0.017). Conclusion: The preoperative GPS predicts adverse survival outcomes in patients with resected stage I NSCLC.
The Regulation of the Metastatic Cascade by Physical Activity: A Narrative Review
Authors: van Doorslaer de ten Ryen Sophie , Deldicque Louise
Published: January 2020

The purpose of this narrative review is to provide an overview of the currently available knowledge about the mechanisms by which physical activity may affect metastatic development. The search terms exercise [Title/Abstract] AND metastasis [Title/Abstract] returned 222 articles on PUBMED on the 10 February 2019. After careful analysis of the abstracts, a final selection of 24 articles was made. Physical activity regulates the levels of metastatic factors in each of the five steps of the process. Moderate intensity exercise appears to prevent tumor spread around the body, among others, by normalizing angiogenesis, destroying circulating tumor cells, and decreasing endothelial cells permeability. Contrarily, high-intensity exercise seems to favor cancer dissemination, likely through excessive stress, which can be somewhat counteracted by an appropriate warm-up. In conclusion, chronic adaptations to moderate-intensity endurance exercise seem the most effective way to achieve a preventive effect of exercise on metastases. Altogether, the data gathered here reinforce the importance of encouraging cancer patients to perform moderate physical activity several times a week. To limit the undesired events thereof, a good knowledge of the patient’s training level is important to establish an adapted exercise training program.
Targeting Glucose Transporters for Breast Cancer Therapy: The Effect of Natural and Synthetic Compounds
Authors: Barbosa Ana M. , Martel Fátima
Published: January 2020

Reprogramming of cellular energy metabolism is widely accepted to be a cancer hallmark. The deviant energetic metabolism of cancer cells-known as the Warburg effect-consists in much higher rates of glucose uptake and glycolytic oxidation coupled with the production of lactic acid, even in the presence of oxygen. Consequently, cancer cells have higher glucose needs and thus display a higher sensitivity to glucose deprivation-induced death than normal cells. So, inhibitors of glucose uptake are potential therapeutic targets in cancer. Breast cancer is the most commonly diagnosed cancer and a leading cause of cancer death in women worldwide. Overexpression of facilitative glucose transporters (GLUT), mainly GLUT1, in breast cancer cells is firmly established, and the consequences of GLUT inhibition and/or knockout are under investigation. Herein we review the compounds, both of natural and synthetic origin, found to interfere with uptake of glucose by breast cancer cells, and the consequences of interference with that mechanism on breast cancer cell biology. We will also present data where the interaction with GLUT is exploited in order to increase the efficiency or selectivity of anticancer agents, in breast cancer cells.
Multimodal Assessment Shows a Mostly Preserved Cognitive Status in Incidentally Discovered Low Grade Gliomas: A Single Institution Study
Authors: Guarracino Ilaria , Ius Tamara , Pegolo Enrico , Cesselli Daniela , Skrap Miran , Tomasino Barbara
Published: January 2020

Incidentally discovered low-grade gliomas (iLGGs) are poorly reported in the literature. Still less is known about iLGG patients’ neuropsychological profile: It is unclear whether iLGG patients are cognitively proficient, thus further confirming the concept of asymptomatic. From our monoinstitutional cohort of 332 patients operated for LGG from 2000 to 2017 we selected those who underwent a neuropsychological testing (n = 217, from 2008 to 2017), and identified 24 young (mean age 38.5 ± 1.06) patients with iLGGs (16 of 24, left hemisphere iLGGs, 8 of 24 right hemisphere iLGGs). The maximum lesions overlap occurred in the left inferior frontal gyrus and in the right anterior cingulate/superior medial frontal gyrus. Patients were cognitively preserved except mild to borderline difficulties in a few of them. The analysis of the equivalent scores (a score laying below or equal to the external nonparametric tolerance limit of adjusted scores corresponding to 0, 1, 2 and 3 are intermediate) highlighted the presence of additional borderline performances. Molecular class correlated with a normal function at visual–spatial intelligence (p = 0.05) and at spatial short-term memory (p = 0.029). Results indicate that at this time of tumor growth, patients’ cognitive abilities are still functional, but are slowly approaching the borderline level.
miR-26a is Involved in Glycometabolism and Affects Boar Sperm Viability by Targeting PDHX
Authors: Wang Wencan , Liang Kai , Chang Yu , Ran Mingxia , Zhang Yan , Ali Malik Ahsan , Dai Dinghui , Qazi Izhar Hyder , Zhang Ming , Zhou Guangbin , Yang Jiandong , Angel Christiana , Zeng Changjun
Published: January 2020

miR-26a is associated with sperm metabolism and can affect sperm motility and apoptosis. However, how miR-26a affects sperm motility remains largely unknown. Our previous study indicated that the PDHX gene is predicted to be a potential target of miR-26a, which is responsible for pyruvate oxidative decarboxylation which is considered as a key step for connecting glycolysis with oxidative phosphorylation. In this study, we first reported a potential relationship between miR-26a and PDHX and their expressions in fresh, frozen-thawed, and epididymal boar sperm. Then, sperm viability and survival were determined after transfection of miR-26a. mRNA and protein expression level of PDHX in the liquid-preserved boar sperm after transfection were also determined by RT-qPCR and Western Blot (WB). Our results showed that expression level of PDHX was significantly increased during sperm transit from epididymal caput to corpus and cauda. Similarly, expression of PDHX was significantly higher (P < 0.05) in fresh sperm as compared to epididymal cauda and frozen-thawed sperm. However, the expression of miR-26a in epididymal corpus sperm was significantly higher (P < 0.05) than that of caput and cauda sperm. Furthermore, after transfection of boar sperm with miR-26a mimic and inhibitor under liquid storage, the lowest and highest sperm viability was observed in miR-26a mimic and inhibitor treatment (P < 0.05), respectively. The protein levels of PDHX, after 24 and 48 h of transfection of miR-26a mimics and inhibitor, were notably decreased and increased (P < 0.05), respectively, as compared to negative control (NC) group. In conclusion, the novel and enticing findings of our study provide a reasonable evidence that miR-26a via PDHX, a link between glycolysis and oxidative phosphorylation, could regulate the glycometabolic pathway which eventually affect boar sperm viability and survival.
IGF-1 Signalling Regulates Mitochondria Dynamics and Turnover through a Conserved GSK-3β–Nrf2–BNIP3 Pathway
Authors: Riis Sarah , Murray Joss B. , O’Connor Rosemary
Published: January 2020

The Insulin-like Growth Factor I (IGF-1) signalling pathway is essential for cell growth and facilitates tumourogenic processes. We recently reported that IGF-1 induces a transcriptional programme for mitochondrial biogenesis, while also inducing expression of the mitophagy receptor BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), suggesting that IGF-1 has a key mitochondria-protective role in cancer cells. Here, we investigated this further and delineated the signaling pathway for BNIP3 induction. We established that IGF-1 induced BNIP3 expression through a known AKT serine/threonine kinase 1 (AKT)-mediated inhibitory phosphorylation on Glycogen Synthase Kinase-3β (GSK-3β), leading to activation of Nuclear Factor Erythroid 2-related Factor 2 (NFE2L2/Nrf2) and acting through the downstream transcriptional regulators Nuclear Respiratory Factor-1 (NRF1) and Hypoxia-inducible Factor 1 subunit α (HIF-1α). Suppression of IGF-1 signaling, Nrf2 or BNIP3 caused the accumulation of elongated mitochondria and altered the mitochondrial dynamics. IGF-1R null Mouse Embryonic Fibroblasts (MEFs) were impaired in the BNIP3 expression and in the capacity to mount a cell survival response in response to serum deprivation or mitochondrial stress. IGF-1 signalling enhanced the cellular capacity to induce autophagosomal turnover in response to activation of either general autophagy or mitophagy. Overall, we conclude that IGF-1 mediated a mitochondria-protective signal that was coordinated through the cytoprotective transcription factor Nrf2. This pathway coupled mitochondrial biogenesis with BNIP3 induction, and increased the cellular capacity for autophagosome turnover, whilst enhancing survival under conditions of metabolic or mitochondrial stress.
Modifications in Tissue and Cell Ultrastructure as Elements of Immunity-Like Reaction in Chenopodium quinoa against Prune Dwarf Virus (PDV)
Authors: Kozieł Edmund , Otulak-Kozieł Katarzyna , Bujarski Józef J.
Published: January 2020

Prune dwarf virus (PDV) is a plant RNA viral pathogen in many orchard trees worldwide. Our knowledge about resistance genes or resistant reactions of plant hosts to PDV is scant. To fill in part of this gap, an aim of this study was to investigate reactions to PDV infection in a model host, Chenopodium quinoa. Our investigations concentrated on morphological and ultrastructural changes after inoculation with PDV strain 0599. It turned out that PDV infection can cause deformations in host cells but also induce changes in the organelles, such as chloroplasts in inoculated leaves. Moreover, we also demonstrated specific reactions/changes, which could be associated with both types of vascular tissue capable of effectively blocking the systemic spread of PDV to upper leaves. Furthermore, the relative amount of virus, P1 protein deposition, and movement protein (MP) gene expression consequently decreased in PDV-inoculated leaves.
Expression of Melatonin and Dopamine D3 Receptor Heteromers in Eye Ciliary Body Epithelial Cells and Negative Correlation with Ocular Hypertension
Authors: Reyes-Resina Irene , Awad Alkozi Hanan , del Ser-Badia Anna , Sánchez-Naves Juan , Lillo Jaume , Jiménez Jasmina , Pintor Jesús , Navarro Gemma , Franco Rafael
Published: January 2020

Background: Experiments in the late nineties showed an inverse relationship in the eye levels of melatonin and dopamine, thereby constituting an example of eye parameters that are prone to circadian variations. The underlying mechanisms are not known but these relevant molecules act via specific cell surface dopamine and melatonin receptors. This study investigated whether these receptors formed heteromers whose function impact on eye physiology. We performed biophysical assays to identify interactions in heterologous systems. Particular heteromer functionality was detected using Gi coupling, MAPK activation, and label-free assays. The expression of the heteroreceptor complexes was assessed using proximity ligation assays in cells producing the aqueous humor and human eye samples. Dopamine D3 receptors (D3Rs) were identified in eye ciliary body epithelial cells. We discovered heteromers formed by D3R and either MT1 (MT1R) or MT2 (MT2R) melatonin receptors. Heteromerization led to the blockade of D3R-Gi coupling and regulation of signaling to the MAPK pathway. Heteromer expression was negatively correlated with intraocular hypertension. Conclusions: Heteromers likely mediate melatonin and dopamine actions in structures regulating intraocular pressure. Significant expression of D3R–MT1R and D3R–MT1R was associated with normotensive conditions, whereas expression diminished in a cell model of hypertension. A clear trend of expression reduction was observed in samples from glaucoma cases. The trend was marked but no statistical analysis was possible as the number of available eyes was 2.
Structural, Thermodiffusive and Thermoelectric Properties of Maghemite Nanoparticles Dispersed in Ethylammonium Nitrate
Authors: Bhattacharya Kakoli , Sarkar Mitradeep , Salez Thomas J. , Nakamae Sawako , Demouchy Gilles , Cousin Fabrice , Dubois Emmanuelle , Michot Laurent , Perzynski Régine , Peyre Véronique
Published: January 2020

Ethylammonium nitrate (ionic liquid) based ferrofluids with citrate-coated nanoparticles and Na + counterions were synthesized for a wide range of nanoparticle (NP) volume fractions ( Φ ) of up to 16%. Detailed structural analyses on these fluids were performed using magneto-optical birefringence and small angle X-ray scattering (SAXS) methods. Furthermore, the thermophoretic and thermodiffusive properties (Soret coefficient S T and diffusion coefficient D m ) were explored by forced Rayleigh scattering experiments as a function of T and Φ . They were compared to the thermoelectric potential (Seebeck coefficient, Se) properties induced in these fluids. The results were analyzed using a modified theoretical model on S T and Se adapted from an existing model developed for dispersions in more standard polar media which allows the determination of the Eastman entropy of transfer ( S ^ NP ) and the effective charge ( Z 0 e f f ) of the nanoparticles.
GPU Computing with Python: Performance, Energy Efficiency and Usability
Authors: Holm Håvard H. , Brodtkorb André R. , Sætra Martin L.
Published: January 2020

In this work, we examine the performance, energy efficiency, and usability when using Python for developing high-performance computing codes running on the graphics processing unit (GPU). We investigate the portability of performance and energy efficiency between Compute Unified Device Architecture (CUDA) and Open Compute Language (OpenCL); between GPU generations; and between low-end, mid-range, and high-end GPUs. Our findings showed that the impact of using Python is negligible for our applications, and furthermore, CUDA and OpenCL applications tuned to an equivalent level can in many cases obtain the same computational performance. Our experiments showed that performance in general varies more between different GPUs than between using CUDA and OpenCL. We also show that tuning for performance is a good way of tuning for energy efficiency, but that specific tuning is needed to obtain optimal energy efficiency.
Development of an Innovative and Eco-Friendly UV Radiation Absorber, Based on Furan Moieties
Authors: Giacomo Busalacchi , Luca Beverina , Nicola Lionetti , Luigi Rigano
Published: January 2020

The increasing concern about the environmental impact of ingredients largely used as sunscreens today is pushing research towards new frontiers for the synthesis and degradation potential of innovative sun protection structures. This study deals with the design, synthesis, efficacy evaluation, and environmental impact of new photo-stable molecules at extended electronic conjugation and which have the ‘charge-transfer’ type of optical transition (push and pull). They efficiently perform absorption of electro-magnetic radiation in the UVB and UVA regions. A preliminary screening, dealing with a whole series of aromatic donors, led to the selection of a key substrate derived from waste material, provided with low environmental impact and largely available on the market. As far as the acceptor was concerned, an ester with cyan-acrylic structure and functionalized with aliphatic chains emerged as the most suitable. This allowed a shift of the λ of absorption in the range of the ultraviolet wavelengths. The synthesis procedures include an acid-catalyst-fitted esterification, condensation, and cold crystallization. It was continuously monitored with suitable characterization instruments (GC-MS, 1H NMR, 13C NMR), in order to avoid by-products and parasitic reactions. The resulting structures (patent pending) are composed by a furan core condensed with cyano-acrylic esters, derivatized with branched chains of hydrocarbons of medium length. The obtained structures are capable of good absorption in the range of wavelengths between 300 and 400 nm. Because of their structures, the new molecules are largely soluble in many common oils and cosmetic ingredients. The new molecules have been successively evaluated in terms of photo-stability, biodegradability, compatibility, and UVB-UVA protection capability, both in-vivo and in-vitro, in comparison with existing sunscreens.
The Genes—Candidates for Prognostic Markers of Metastasis by Expression Level in Clear Cell Renal Cell Cancer
Authors: Apanovich Natalya , Peters Maria , Apanovich Pavel , Mansorunov Danzan , Markova Anna , Matveev Vsevolod , Karpukhin Alexander
Published: January 2020

The molecular prognostic markers of metastasis are important for personalized approaches to clear cell renal cell carcinoma (ccRCC) treatment but markers for practical use are still missing. To address this gap we studied the expression of ten genes—CA9, NDUFA4L2, VWF, IGFBP3, BHLHE41, EGLN3, SAA1, CSF1R, C1QA, and FN1—through RT-PCR, in 56 ccRCC patients without metastases and with metastases. All of these, excluding CSF1R, showed differential and increased (besides SAA1) expression in non-metastasis tumors. The gene expression levels in metastasis tumors were decreased, besides CSF1R, FN1 (not changed), and SAA1 (increased). There were significant associations of the differentially expressed genes with ccRCC metastasis by ROC analysis and the Fisher exact test. The association of the NDUFA4L2, VWF, EGLN3, SAA1, and C1QA expression with ccRCC metastasis is shown for the first time. The CA9, NDUFA4L2, BHLHE4, and EGLN3 were distinguished as the strongest candidates for ccRCC metastasis biomarkers. We used an approach that presupposed that the metastasis marker was the expression levels of any three genes from the selected panel and received sensitivity (88%) and specificity (73%) levels with a relative risk of RR > 3. In conclusion, a panel of selected genes—the candidates in biomarkers of ccRCC metastasis—was created for the first time. The results might shed some light on the ccRCC metastasis processes.
Propagation of Overvoltages in the Form of Impulse, Chopped and Oscillating Waveforms in Transformer Windings—Time and Frequency Domain Approach
Authors: Florkowski Marek , Furgał Jakub , Kuniewski Maciej
Published: January 2020

This paper describes a comparison of overvoltage propagation in transformer windings. Expanding and evolving electrical networks comprise various classes of transient waveforms, related to network reconfigurations, failure stages and switching phenomena, including new sources based on power electronics devices. In particular, the integration of renewable energy sources—mainly solar and wind—as well as expanding charging and energy storage infrastructure for electric cars in smart cities results in network flexibility manifested by switching phenomena and transients propagation, both impulse and oscillating. Those external transients, having a magnitude below the applied protection level may have still a considerable effect on winding electrical insulation in transformers, mainly due to internal resonance phenomena, which have been the root cause of many transformer failures. Such cases might occur if the frequency content of the incoming waveform matches the resonance zones of the winding frequency characteristic. Due to this coincidence, the measurements were performed both in time and frequency domain, applying various classes of transients, representing impulse, chopped (time to chopping from 1 µs to 50 µs) and oscillating overvoltages. An additional novelty was a superposition of a full lighting impulse with an oscillating component in the form of a modulated wavelet. The comparison of propagation of those waveforms along the winding length as well as a transfer case between high and low voltage windings were analyzed. The presented mapping of overvoltage prone zones along the winding length can contribute to transformer design optimization, development of novel diagnostic methodology, improved protection concepts and the proper design of modern networks.
Study on Intelligent Identification Method of Coal Pillar Stability in Fully Mechanized Caving Face of Thick Coal Seam
Authors: Dai Jingjing , Shan Pengfei , Zhou Qi
Published: January 2020

The combination of coal precise mining and information technology in the new century is one of the important directions for the future development of coal mining. Taking the fully mechanized top coal caving condition of a thick coal seam in the 90,101 working face of Baoshan Yujing Coal Mine in Shanyin City, Shanxi Province as an example, the intelligent identification method of section coal pillar stability was studied. The load transfer law of overlying strata in the upper part of coal pillar was analyzed, and the coal pillar values of each index were obtained by using an empirical formula, mean impact value-genetic algorithm-BP neural network (MIV-GA-BP) simulation experiment, and finite difference algorithm. The Delphi index evaluation system was used to calculate the optimal value of the coal pillar. The results showed that the non-contact cantilevered triangle on the two wings of the coal pillar in the goaf reduced the stress on the coal pillar; according to the width of the coal pillar at 10 m, 14 m, 16 m, and 20 m, combined with the relationship between the plastic zone and the core zone of coal pillar, and the relationship between the stress field and the ultimate strength of coal pillar, the numerical simulation value of the coal pillar was determined. The MIV (mean impact value) characteristics screened out the influencing factors of coal pillar width in the section near the horizontal fully mechanized top coal caving face order of importance; the relative error between the predicted value and the expected value of the MIV-GA-BP simulation experiment was less than 5%, which has good stability for the multi-factor nonlinear coupling prediction problem; and the optimal value of the coal pillar was 16.03 m by the intelligent identification method of the coal pillar. When the 16 m pillar was used, the surrounding rock deformation of the roadway was small, and the control effect was good. The research results provide a theoretical basis and reference for the parameter determination of similar projects.
Analysis of Climate Mitigation Technology and Finance in Relation to Multilateral Development Banks
Authors: Lee Joo Young , Han Su Hyeon
Published: January 2020

This paper looks at the current state of multilateral development banks (MDBs) for climate change measures and the funding status of those invested in mitigation technology in order to briefly review the current outcome of the technology transfer and financial support. In other words, the aim of this study is to collect and analyze information about the current status of total investment in the field of technology for mitigating GHGs (Greenhouse Gases) from MDBs and identify implications of the status. In this study, a screening technique has been used three times to make a database for project information in the field of mitigation of climate change. So far, based on the finalized DB (Database), mitigation technology projects supported by MDBs have been investigated; based on the result, a connected analysis has been conducted between MDBs, mitigation technology, and countries. According to the derived current status, project support in renewable energy and energy demand areas turned out to be the highest at 75% of the entire mitigation technology. Rather than the renewable energy and energy demand areas where climate technology projects have frequently been performed throughout the world, it was confirmed that long-term climate technology projects for GHG fixation were being performed. According to the results of comparison and analysis of countries with high GHG emissions and their centrality, centrality turned out to be high in the field of GHG fixation in China, the country with the highest GHG emissions. This seems to indicate that countries emitting a substantial amount of GHGs will invest more on projects in the field of GHG fixation as well as on projects on renewable energy. Thus, this study is expected to contribute to understanding the trends of climate technology projects for coping with climate change and using them in establishing future policies on climate technology. In addition, it is expected to be used as a reference for countries with insufficient investment in climate technology despite the high Climate Risk Index (CRI).
A Novel Energy Recovery System Integrating Flywheel and Flow Regeneration for a Hydraulic Excavator Boom System
Authors: Li Jiansong , Zhao Jiyun , Zhang Xiaochun
Published: January 2020

Implementing an energy recovery system (ERS) is an effective solution to improve energy efficiency for hydraulic excavators (HEs). A flywheel energy recovery system (FERS) is proposed based on this concept. A hydraulic pump motor (PM) is employed as the energy conversion component and a flywheel is used as the energy storage component. Since the pressure is low because the bucket is usually empty as the boom lowers, a relatively large PM should be used in the FERS. To overcome this drawback, a novel compound energy recovery system integrating flywheel and flow regeneration (FFERS) is proposed in this paper. The working principle of the system is analyzed in detail. The introduction of flow regeneration has two benefits; one is downsizing the displacement of PM and the other one is an extra improvement of energy efficiency. The primary parameters of both are matched based on a 4 t excavator. Compared with the PM used in the FERS, the PM displacement in the FFERS is reduced by 71%. For comparison, a general model that can operate in either the FERS mode or the FFERS mode is developed in AMESim. The modeling results show that the FFERS with a downsized PM contributes a 13% increase in energy recovery and reutilization efficiency (62%) as compared with the FERS.
Seed Funds Leverage External Awards for Research in Natural Resources and Agricultural Systems
Authors: Davis John M. , Soltis Pamela S. , Adams Damian C. , Larkin Sherry L. , Gilbert Robert A.
Published: January 2020

In the United States, “seed fund” programs in biomedical fields have been reported to enhance faculty success in securing federal awards. Seed funds are relatively small internal grants that are allocated by universities to their faculty to invigorate research, in anticipation that they will lead to larger benefits—these benefits could be the creation of stronger teams that lead to larger external grants and/or more impactful publications. We hypothesized that a seed funding approach for research on natural resources and agricultural systems, including forest systems, may have similar benefits. Here, we report that seed funding supported faculty development in numerous ways, including leveraging to generate external competitive awards. For each dollar of institutional investment in each of the three seed funding programs, faculty acquired $6, >$50, and $4.50, respectively, in external funding that was leveraged within three years of the seed fund award date. Notably, the majority of the leveraged funding was from federal granting agencies through competitive programs. Federal competitive awards are important elements of tenure and promotion dossiers, as they reflect success in acquiring grants that are difficult to obtain, thus providing evidence of research excellence. We speculate that the seed fund proposal review process prepared faculty for competitive awards. Finally, we suggest best management practices for the implementation of seed funding to support teams of faculty.
Study on Bridge Displacement Monitoring Algorithms Based on Multi-Targets Tracking
Authors: Wang Jiajia , Li Guangming
Published: January 2020

Bridge displacement measurement is an important area of bridge health monitoring, which can directly reflect whether the deformation of bridge structure exceeds its safety permission. Target tracking technology and Digital Image Correlation (DIC) are two fast-developing and well-known methods for non-contact bridge displacement monitoring in Digital Image Processing (DIP) methods. The former’s cost of erecting detection equipment is too large for bridges with a large span that need to locate more multi-targets because of its tracking only one target on a camera while the latter is not suitable for remote detection because it requires very high detection conditions. After investigating the evolution of bridge displacement monitoring, this paper proposes a bridge displacement monitoring algorithm based on multi-target tracking. The algorithm takes full account of practical application and realizes accuracy, robustness, real-time, low-cost, simplicity, and self-adaptability, which sufficiently adapts the bridge displacement monitoring in theory.
Recent Advances in Mechanics of Non-Newtonian Fluids
Authors: Wu Wei-Tao , Massoudi Mehrdad
Published: January 2020

Flow of non-Newtonian (non-linear) fluids occurs not only in nature, for example, mud slides and avalanches, but also in many industrial processes involving chemicals (polymers), biological materials (blood), food (honey, ketchup, yogurt), pharmaceutical and personal care items (shampoo, creams), etc [...]
Shape Optimization of a Two-Fluid Mixing Device Using Continuous Adjoint
Authors: Alexias Pavlos , Giannakoglou Kyriakos C.
Published: January 2020

In this paper, the continuous adjoint method is used for the optimization of a static mixing device. The CFD model used is suitable for the flow simulation of the two miscible fluids that enter the device. The formulation of the adjoint equations, which allow the computation of the sensitivity derivatives is briefly demonstrated. A detailed analysis of the geometry parameterization is presented and a set of different parameterization scenarios are investigated. In detail, two different parameterizations are combined into a two-stage optimization algorithm which targets maximum mixture uniformity at the exit of the mixer and minimum total pressure losses. All parameterizations are in conformity with specific manufacturability constraints of the final shape. The non-dominated front of optimal solutions is obtained by using the weighted sum of the two objective functions and executing a set of optimization runs. The effectiveness of the proposed synthetic parameterization schemes is assessed and discussed in detail. Finally, a reduced length mixer is optimized to study the impact of the length of the tube on the device’s performance.
Enzymatic Reaction-Related Protein Degradation and Proteinaceous Amino Acid Metabolism during the Black Tea (Camellia sinensis) Manufacturing Process
Authors: Chen Yiyong , Zeng Lanting , Liao Yinyin , Li Jianlong , Zhou Bo , Yang Ziyin , Tang Jinchi
Published: January 2020

Amino acids contribute to the nutritional value and quality of black tea. Fermentation is the most important stage of the black tea manufacturing process. In this study, we investigated protein degradation and proteinaceous amino acid metabolism associated with enzymatic reactions during fermentation in the black tea manufacturing process. The results showed that the concentrations of both protein and free amino acids decreased during fermentation. We also confirmed that proteins were broken down into free amino acids by artificially synthesized dipeptide benzyloxycarbonyl glutamyl-tyrosine (Z-Glu-Tyr). Metabolites of the amino acid metabolic pathway increased significantly during fermentation. Furthermore, we confirmed that free amino acids were degraded to volatile compounds in a tracer experiment with the isotope precursor. These results provide information that will help black tea manufacturers improve the quality of black tea.
A Real-Time PCR Screening Assay for Rapid Detection of Listeria Monocytogenes Outbreak Strains
Authors: Torresi Marina , Ruolo Anna , Acciari Vicdalia Aniela , Ancora Massimo , Blasi Giuliana , Cammà Cesare , Centorame Patrizia , Centorotola Gabriella , Curini Valentina , Guidi Fabrizia , Marcacci Maurilia , Orsini Massimiliano , Pomilio Francesco , Di Domenico Marco
Published: January 2020

From January 2015 to March 2016, an outbreak of 23 human cases of listeriosis in the Marche region and one human case in the Umbria region of Italy was caused by Listeria monocytogenes strains showing a new pulsotype never described before in Italy. A total of 37 clinical strains isolated from patients exhibiting listeriosis symptoms and 1374 strains correlated to the outbreak were received by the Italian National Reference Laboratory for L. monocytogenes (It NRL Lm) of Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise (IZSAM) for outbreak investigation. A real-time PCR assay was purposely designed for a rapid screening of the strains related to the outbreak. PCR-positive strains were successively typed through molecular serogrouping, pulsed field gel electrophoresis (PFGE), and Next Generation Sequencing (NGS). Applying the described strategy, based on real-time PCR screening, we were able to considerably reduce time and costs during the outbreak investigation activities.
Effect of Selenium Source and Level on Performance, Egg Quality, Egg Selenium Content, and Serum Biochemical Parameters in Laying Hens
Authors: Liu Hu , Yu Qifang , Fang Chengkun , Chen Sijia , Tang Xiaopeng , Ajuwon Kolapo M. , Fang Rejun
Published: January 2020

The objective of this study was to compare the effect of sodium selenite (SS) and selenium yeast (SY) on performance, egg quality, and selenium concentration in eggs and serum biochemical indices in laying hens. Seven hundred twenty healthy Roman laying hens (21 weeks old, 18 weeks in lay) with a similar laying rate (90.27% ± 1.05%) were randomly divided into 5 groups with 6 replicates of 24 hens each. Five diets were prepared as a 1+2×2 factorial arrangement with control and two sources of Se at two levels. Control diet (control) was prepared without adding exogenous selenium (analyzed basal Se content of 0.178 mg/kg). The other four diets were prepared with the control diet supplemented with SY or SS at 0.3 mg/kg (low; L) or 0.5 mg/kg (high; H) to give 5 diets designated as control, SY-L, SY-H, SS-L, and SS-H. The analyzed selenium content in the SY-L, SY-H, SS-L, and SS-H diets were 0.362, 0.572, 0.323, and 0.533 mg/kg respectively. The pre-trial period lasted 7 d, and the experimental period lasted 56 d (30 weeks old), during which the egg production, egg quality, and hen serum parameters were measured. Results showed that selenium source and level had no effect (P > 0.05) on average daily egg weight and feed conversion ratio (FCR). However, the laying rate was different at the L and H levels of supplementation, regardless of source, such that hens that were supplemented had a higher performance than that of the control, and the H level of supplementation lead to a higher laying rate than that of the L level (P < 0.05). There was a difference in average daily feed intake (ADFI) with an interaction in selenium source and level (P < 0.05), such that SS-L was higher than other selenium supplemented treatment or control. There were no significant differences in egg quality (P > 0.05); at the high level, SY had higher egg yolk selenium compared with SS. However, within SY, adding 0.5 mg/kg selenium led to higher egg yolk selenium than 0.3 mg/kg selenium (P < 0.05). Moreover, adding 0.3 mg/kg SY, 0.3 mg/kg, or 0.5 mg/kg SS to the basal diet had no significant effect on the selenium content in the egg (P > 0.05). There were no significant differences in serum biochemical indices among the five groups (P > 0.05). In conclusion, adding a high level of selenium in the diet of laying hens significantly increased egg production, and addition of a high level of selenium in the form of SY led to a higher deposition of selenium in the yolk than that of SS. These results indicate that adding 0.5 mg/kg of SY in the diet of laying hens would result in Se-enriched eggs.
Practice-Based Research in Complementary Medicine: Could N-of-1 Trials Become the New Gold Standard?
Authors: Bradbury Joanne , Avila Cathy , Grace Sandra
Published: January 2020

Complementary medicines and therapies are popular forms of healthcare with a long history of traditional use. Yet, despite increasing consumer demand, there is an ongoing exclusion of complementary medicines from mainstream healthcare systems. A lack of evidence is often cited as justification. Until recently, high-quality evidence of treatment efficacy was defined as findings from well-conducted systematic reviews and meta-analyses of randomized controlled trials. In a recent and welcome move by the Oxford Centre for Evidence-Based Practice, however, the N-of-1 trial design has also been elevated to the highest level of evidence for treatment efficacy of an individual, placing this research design on par with the meta-analysis. N-of-1 trial designs are experimental research methods that can be implemented in clinical practice. They incorporate much of the rigor of group clinical trials, but are designed for individual patients. Individualizing treatment interventions and outcomes in research designs is consistent with the movement towards patient-centered care and aligns well with the principles of holism as practiced by naturopaths and many other complementary medicine practitioners. This paper explores whether rigorously designed and conducted N-of-1 trials could become a new ‘gold standard’ for demonstrating treatment efficacy for complementary medicine interventions in individual patients in clinical practice.
Comparison between Continuous and Fractionated Game Format on Internal and External Load in Small-Sided Games in Soccer
Authors: Branquinho Luís , Ferraz Ricardo , Travassos Bruno , C. Marques Mário
Published: January 2020

This study aimed to identify the effects of continuous and fractionated game formats on internal and external load in small-sided games in soccer. Twenty male professional soccer players participated in the study performing the same exercise (5 vs. 5 players) continuously (1 × 24 min) and in a repeated/fractioned manner (2 × 12 min, 4 × 6 min, and 6 × 4 min). A comparison between playing conditions was assessed by means of standardized mean differences calculated with combined variance and respective confidence intervals of 90%. The limits for the statistics were 0.2, trivial; 0.6, small; 1.2, moderate; 2.0, large; and >2.0, very large. The results indicate that the use of the continuous method seems to present the tendency of less physical impact on the internal and external loads compared to the fractionated method. In addition, the higher number of exercise repetitions in the fractionated method was found to increase the external load compared to the continuous method. This study showed that application of small-sided games by the fractionated method tends to result in higher training loads.
Diurnal and Seasonal Variations in the Effect of Urban Environmental Factors on Air Temperature: A Consecutive Regression Analysis Approach
Authors: Ha Jaehyun , Choi Yeri , Lee Sugie , Oh Kyushik
Published: January 2020

This study investigates the diurnal and seasonal variations in the effect of environmental features on air temperature in Seoul, Korea. We expect that this study will lead to the identification of factors that can be applied for urban heat island mitigation strategies in summer without leading to an unintended result in winter. As our dependent variable, we employed the smoothed 31-day moving average of air temperatures, where we controlled the seasonal variation by normalizing the values observed from 247 automatic weather stations (AWS) from 2015 to 2016. Subsequently, we conducted consecutive log–log regression analyses of each day to examine patterns of change in regression coefficients and the significance of each independent variable. For independent variables, we applied built environment features including albedo, land-use, average building floors, the sky view factor, and green and water areas. This study provides analytical results regarding the relationship between environmental factors and air temperature. This study also addresses imperative issues for planners, especially regarding albedo, wind path, building geometry, and land use types. Finally, this study gives useful insights for managing the diurnal and seasonal variations of urban thermal environment in the mega-city.