bluuebunny/crossref_metadata_2025 huggingface dataset:
Microtubule Organization in Striated Muscle Cells
Authors: Becker Robert , Leone Marina , Engel Felix
Published: June 2020

Distinctly organized microtubule networks contribute to the function of differentiated cell types such as neurons, epithelial cells, skeletal myotubes, and cardiomyocytes. In striated (i.e., skeletal and cardiac) muscle cells, the nuclear envelope acts as the dominant microtubule-organizing center (MTOC) and the function of the centrosome—the canonical MTOC of mammalian cells—is attenuated, a common feature of differentiated cell types. We summarize the mechanisms known to underlie MTOC formation at the nuclear envelope, discuss the significance of the nuclear envelope MTOC for muscle function and cell cycle progression, and outline potential mechanisms of centrosome attenuation.
The Meaning of Immune Reconstitution after Alemtuzumab Therapy in Multiple Sclerosis
Authors: Rolla Simona , Maglione Alessandro , De Mercanti Stefania Federica , Clerico Marinella
Published: June 2020

Alemtuzumab is a monoclonal antibody that binds to CD52, a protein present on the surface of mature lymphocytes, but not on the stem cells from which these lymphocytes are derived. It is currently used as an immune reconstitution therapy in patients with relapsing–remitting multiple sclerosis. Alemtuzumab treatment is an intermittent infusion that induces long-term remission of Multiple Sclerosis also in the treatment-free period. After the robust T and B cell depletion induced by alemtuzumab, the immune system undergoes radical changes during its reconstitution. In this review, we will discuss the current knowledge on the reconstitution of the lymphocyte repertoire after alemtuzumab treatment and how it could affect the development of side effects, which led to its temporary suspension by the European Medical Agency.
Healing Performance of a Self-Healing Protective Coating According to Damage Width
Authors: Kim Dong-Min , Lee Junseo , Choi Ju-Young , Jin Seung-Won , Nam Kyeong-Nam , Park Hyeong-Ju , Lee Seung-Hyun , Chung Chan-Moon
Published: June 2020

Although self-healing protective coatings have been widely studied, systematic research on healing performance of the coating according to damage width has been rare. In addition, there has been rare reports of self-healing of the protective coating having damage width wider than 100 µm. In this study, self-healing performance of a microcapsule type self-healing protective coating on cement mortar was studied for the coating with damage width of 100–300 µm. The effect of capsule-loading (20 wt%, 30 wt% and 40 wt%), capsule size (65-, 102- and 135-µm-mean diameter) and coating thickness (50-, 80- and 100-µm-thick undercoating) on healing efficiency was investigated by water sorptivity test. Accelerated carbonation test, chloride ion penetration test and scanning electron microscope (SEM) study were conducted for the self-healing coating with a 300-µm-wide damage. Healing efficiency of the self-healing coating decreased with increasing damage width. As capsule-loading, capsule size or coating thickness increased, healing efficiency of the self-healing coating increased. Healing efficiency of 76% or higher was achieved using the self-healing coating with a 300-µm-wide scratch. The self-healing coating with a 200-µm-wide crack showed healing efficiency of 70% or higher. The self-healing coating having a 300-µm-wide scratch showed effective protection of the substrate mortar from carbonation and chloride ion penetration, which was supported by SEM study.
Effect of Interlayer Cooling on the Preparation of Ni-Based Coatings on Ductile Iron
Authors: He Yuyu , Liu Yijian , Yang Jiquan , Xie Fei , Huang Wuyun , Zhu Zhaowei , Cheng Jihong , Shi Jianping
Published: June 2020

In metal additive manufacturing without interlayer cooling, the macro-size of the layer itself is difficult to control due to the thermal storage effect. The effect of interlayer cooling was studied by cladding Ni-based coatings on the substrate of ductile iron. The results show that under the same process parameters, compared with non-interlayer cooling deposition, the dilution rate is better, and the thickness increase of interlayer cooling deposition is more uniform, which is conducive to controlling the macro-size of the interlayer cooling deposition. Furthermore, interlayer cooling deposition has fewer impurities and more uniform microstructures. Moreover, the average grain size is refined and the dendrite growth is inhibited, which improves the mechanical properties of the coating. Therefore, the hardness of the interlayer cooling specimens is greater than that of the non-interlayer-cooled specimens.
Impact of mRNA-Assessed Molecular Subtype Conversion, Intact and Apoptotic Circulating Tumor Cells on Survival of Metastatic Breast Cancer Patients: Proof of Principle
Authors: Stefanovic Stefan , Deutsch Thomas M. , Wirtz Ralph , Hartkopf Andreas , Sinn Peter , Kohler Maximilian , Hofmann Jan , Bankovic Sanja , Vassilev Katja , Sütterlin Marc , Schneeweiss Andreas , Wallwiener Markus
Published: June 2020

Breast cancers (BC) can mutate, allowing metastatic tumors (MT) to sometimes differ to primary tumors (PT) in gene expression. Despite contemporary metastatic breast cancer (MBC) therapy, subtype conversion seems prognostically disadvantageous. We strived to determine the influence of mRNA-assessed intrinsic subtype stability comparing PT and MT biopsies and circulating tumor cell (CTC)-based liquid biopsies on progression free survival (PFS) and overall survival (OS). Additional analyzed prognostic factors were PT subtype, MT subtype and hormone receptor loss. Kaplan-Meier curves and the log rank tests were used to compare PFSs and OSs. The proportions of luminal B and triple negative subtype MTs were increased compared to those observed in PTs. Fifteen patients were found to have tumors that underwent intrinsic subtype conversion and their OS was significantly decreased (p = 0.038). No such difference was observed when it comes to PFS. The majority of these tumors switched to a more aggressive intrinsic subtype. No significant differences in PFSs or OSs were observed between subtype converters with triple negative PTs compared to those with luminal subtype PTs. The same is true of subtype stable patients. Total CTC, iCTC and aCTC counts decreased with therapy, but there were no significant differences between subtype converters and subtype stable patients. Our data confirm a poorer overall survival of the intrinsic subtype converters and emphasize the importance of acquiring biopsies and re-biopsies of all available metastatic lesions alongside with CTC-based liquid biopsies for earlier recognition of patients with poorer prognosis and in need of altered individualized therapy regimens.
KCTD15 Protein Expression in Peripheral Blood and Acute Myeloid Leukemia
Authors: Smaldone Giovanni , Coppola Luigi , Incoronato Mariarosaria , Parasole Rosanna , Ripaldi Mimmo , Vitagliano Luigi , Mirabelli Peppino , Salvatore Marco
Published: June 2020

Leukocytes are major cellular components of the inflammatory and immune response systems. After their generation in the bone marrow from hematopoietic stem cells, they maturate as granulocytes (neutrophils, eosinophils, and basophils), monocytes, and lymphocytes. The abnormal accumulation and proliferation of immature blood cells (blasts) lead to severe and widespread diseases such as leukemia. We have recently shown that KCTD15, a member of the potassium channel tetramerization domain containing protein family (KCTD), is remarkably upregulated in leukemic B-cells. Here, we extend our investigation by monitoring the KCTD15 expression levels in circulating lymphocytes, monocytes, and granulocytes, as well as in leukemia cells. Significant differences in the expression level of KCTD15 were detected in normal lymphocytes, monocytes, and granulocytes. Interestingly, we also found overexpression of the protein following leukemic transformation in the case of myeloid cell lineage. Indeed, KCTD15 was found to be upregulated in K562 and NB4 cells, as well as in HL-60 cell lines. This in vitro finding was corroborated by the analysis of KCTD15 mRNA of acute myeloid leukemia (AML) patients reported in the Microarray Innovations in Leukemia (MILE) dataset. Collectively, the present data open interesting perspectives for understanding the maturation process of leukocytes and for the diagnosis/therapy of acute leukemias.
Single DC Source Multilevel Inverter with Changeable Gains and Levels for Low-Power Loads
Authors: Samadaei Emad , Salehi Aidin , Iranian Mina , Pouresmaeil Edris
Published: June 2020

Different types of multilevel converters have been used to convert DC voltage to AC voltage for different applications. It is, however, desirable for flexible AC output voltage to be created from a single DC source at lower cost. This paper presents a new type of single DC source multilevel inverter which has the ability to create a different number of levels with low rate components. This device is also able boost the magnitude of the output voltage by means of a variable gain with one DC source. The advantages of the multilevel inverter are rapid stepping among levels, and its ability to produce different ranges of levels (seven, nine and eleven) and gains (two, four and eight). The proposed multilevel inverter includes six semiconductor switches, eight diodes, two capacitors and two inductors, making it suitable for low-power applications. A simulation in MATLAB and experimental tests on a prototype setup showed good performance for different modulations, with THD% down to 2.29%, which are meets the IEEE standard (IEEE 519).
Performance of Kernel Estimator and Johnson SB Function for Modeling Diameter Distribution of Black Alder (Alnus glutinosa (L.) Gaertn.) Stands
Authors: Pogoda Piotr , Ochał Wojciech , Orzeł Stanisław
Published: June 2020

We compare the usefulness of nonparametric and parametric methods of diameter distribution modeling. The nonparametric method was represented by the new tool—kernel estimator of cumulative distribution function with bandwidths of 1 cm (KE1), 2 cm (KE2), and bandwidth obtained automatically (KEA). Johnson SB (JSB) function was used for the parametric method. The data set consisted of 7867 measurements made at breast height in 360 sample plots established in 36 managed black alder (Alnus glutinosa (L.) Gaertn.) stands located in southeastern Poland. The model performance was assessed using leave-one-plot-out cross-validation and goodness-of-fit measures: mean error, root mean squared error, Kolmogorov–Smirnov, and Anderson–Darling statistics. The model based on KE1 revealed a good fit to diameters forming training sets. A poor fit was observed for KEA. Frequency of diameters forming test sets were properly fitted by KEA and poorly by KE1. KEA develops more general models that can be used for the approximation of independent data sets. Models based on KE1 adequately fit local irregularities in diameter frequency, which may be considered as an advantageous in some situations and as a drawback in other conditions due to the risk of model overfitting. The application of the JSB function to training sets resulted in the worst fit among the developed models. The performance of the parametric method used to test sets varied depending on the criterion used. Similar to KEA, the JSB function gives more general models that emphasize the rough shape of the approximated distribution. Site type and stand age do not affect the fit of nonparametric models. The JSB function show slightly better fit in older stands. The differences between the average values of Kolmogorov–Smirnov (KS), Anderson–Darling (AD), and root mean squared error (RMSE) statistics calculated for models developed with test sets were statistically nonsignificant, which indicates the similar usefulness of the investigated methods for modeling diameter distribution.
Sea Level Trend and Fronts in the South Atlantic Ocean
Authors: Ruiz-Etcheverry Laura A. , Saraceno Martin
Published: June 2020

The understanding of the physical drivers of sea level trend is crucial on global and regional scales. In particular, little is known about the sea level trend in the South Atlantic Ocean in comparison with other parts of the world. In this work, we computed the South Atlantic mean sea level (SAMSL) trend from 25 years of satellite altimetry data, and we analyzed the contributions of steric height (thermosteric and halosteric components) and ocean mass changes for the period 2005–2016 when all the source data used (Argo, GRACE and satellite altimetry) overlap. The SAMSL trend is 2.65 ± 0.24 mm/yr and is mostly explained by ocean mass trend, which is 2.22 ± 0.21 mm/yr. However, between 50° S–33° S, the steric height component constitutes the main contribution in comparison with the ocean mass component. Within that latitudinal band, three regions with trend values higher than the SAMSL trend are observed when considering 25 years of satellite SLA. In the three regions, a southward displacement of the Subtropical, Subantarctic, and Polar Fronts is observed. The southward shift of the fronts is associated with the strengthening and polar shift of westerly winds and contributes to a clear thermosteric trend that translates to the SLA trend observed in those regions.
Counting Form: Gender and the Geometries of Address, in Frances Presley and Carol Watts
Authors: Entwistle Alice
Published: June 2020

This essay treats two innovative site-specific sequences produced by women in the first decade of the twenty first century. Both are explicitly interested in the relationship between geometry, writing (as material and political practice) and geo-cultural space, a relationship each finds inflected to some extent by gender emphases. Starting from the premise that any piece of writing is itself a place, the essay considers the self-conscious textualities of its primary texts—one concerned with Exmoor; the other with a sheep-farm in rural mid-Wales—in the light of their different, if similarly rural and relatively remote, contexts. Presley’s ‘Stone Settings’ explores the relationship between some of the quasi-geometrical Neolithic stone arrangements dotted across Exmoor, and the mediation of their apparently Euclidean sometimes barely visible forms in/as text. Watts’ work-in-progress Zeta Landscape mobilises in the ‘analytical’ or ‘projective’ (ie non-Euclidean) geometry of its title the complex weave of routine care-giving and accountancy charging the contemporary (Michel Foucault’s ‘distributive’) pastoral. Both sequences wryly suggest that poetic form can finally no more adequately figure place than the abstractions of mathematical discourse can utter the cultural ecology of any environment, however concrete-seeming. Aided by Jacques Derrida’s powerful essay ‘White Mythology’, the account comes to rest on the equally equivocal recognition of the in/effectuality of metaphor in any kind of address, critical or creative.
Radical Togetherness: African-American Literature and Abolition Pedagogy at Parchman and Beyond
Authors: Alexander Patrick Elliot
Published: June 2020

This article makes the case that the student-centered learning paradigm that I have aimed to establish at Parchman/Mississippi State Penitentiary as a member of a college-in-prison program represents a prison abolition pedagogy that builds on Martin Luther King and Angela Y. Davis’s coalitional models of abolition work. Drawing from Davis’s abolition-framed conception of teaching in jails and prisons as expressed in her autobiography and her critical prison studies text Are Prisons Obsolete?, I argue that the learning environments that I create collaboratively with students at Parchman similarly respond to incarcerated students’ institution-specific concerns and African-American literary interests in ways that lessen, if only temporarily, the social isolation and educational deprivation that they routinely experience in Mississippi’s plantation-style state penitentiary. Moreover, I am interested in the far-reaching implications of what I have theorized elsewhere as “abolition pedagogy”—a way of teaching that exposes and opposes the educational deprivation, under-resourced and understaffed learning environments, and overtly militarized classrooms that precede and accompany too many incarcerations. As such, this article also focuses on my experience of teaching about imprisonment in African-American literature courses at the University of Mississippi at the same time that I have taught classes at Parchman that honor the African-American literary interests of imprisoned students there.
The New German Nature Lyric
Authors: Thomas Nicola
Published: June 2020

Naturlyrik has long been a contested category in German poetry, but however politically suspect some may find ‘Gespräch(e) über Bäume’ (Brecht), they are vitally important in the era of anthropogenic environmental collapse. The current generation of German-language poets have sought new ways of writing about the natural world and environments; these differ from, and draw on, pre-twentieth-century Naturlyrik as well as the complex, often critical, representations of nature in poetry after the Second World War. Representations of gardens and other human-‘managed’ natural spaces, references to and rewritings of German literary tradition, and the exploration of non-human voices and subjects all serve as means of restoring subjective fullness and complexity to Naturlyrik. The questions of voice and form which are central to the idea of the lyric genre as a whole are implicated in the development of a contemporary nature poetry beyond both Brecht and Benn, and Anthropocene Naturlyrik is pushing German lyric poetry itself into a new phase.
The Psycho-Social Impact of Human-Animal Interactions
Authors: Fine Aubrey H.
Published: June 2020

When I was asked by the Journal to guest edit a special issue on the psychosocial impact of human-animal interactions, I was honored to accept the invitation [...]
Sylvatic Mosquito Diversity in Kenya—Considering Enzootic Ecology of Arboviruses in an Era of Deforestation
Authors: Eastwood Gillian , Sang Rosemary C. , Lutomiah Joel , Tunge Philip , Weaver Scott C.
Published: June 2020

As new and re-emerging vector-borne diseases are occurring across the world, East Africa represents an interesting location, being the origin of several arboviruses with a history of urbanization and global spread. Rapid expansion of urban populations and alteration of natural habitats creates the opportunity for arboviruses to host-switch from wild, sylvatic hosts or vectors into urban transmission affecting human populations. Although mosquito surveillance regularly takes place in urban areas of Kenya, for example identifying vectors of dengue virus or malaria viruses, little work has been carried out to determine the distribution and abundance of sylvatic vectors. Here, we describe the mosquito vector species and diversity collected at twelve forest habitats of rural Kenya. We conducted arbovirus screening of over 14,082 mosquitoes (47 species, 11 genera) as 1520 pools, and detected seven viruses (six bunyaviruses, and one flavivirus-bunyavirus co-infection) isolated from pools of Aedes dentatus, Anopheles funestus, Culex annulioris, and Cx. vansomereni. Awareness of sylvatic vector species and their location is a critical part of understanding the ecological foci and enzootic cycling of pathogens that may be of concern to public, animal or wildlife health. As natural ecosystems come under anthropogenic pressures, such knowledge can inform us of the One Health potential for spillover or spillback leading to outbreaks, and assist in vector control strategies.
Immune Checkpoint Inhibitors: A Promising Choice for Endometrial Cancer Patients?
Authors: Musacchio Lucia , Boccia Serena Maria , Caruso Giuseppe , Santangelo Giusi , Fischetti Margherita , Tomao Federica , Perniola Giorgia , Palaia Innocenza , Muzii Ludovico , Pignata Sandro , Benedetti Panici Pierluigi , Di Donato Violante
Published: June 2020

Although around 80% of endometrial cancers are diagnosed at early stages and present with a 5-year survival rate exceeding 95%, patients with advanced and recurrent disease show a poor prognosis and low response rates to standard chemotherapy. In the era of targeted therapy, the great advances in the understanding of programmed death-ligand 1 (PD-L1) upregulation in cancer cells, which is responsible for tumor immune escape, have contributed to the increasing interest in immune checkpoint inhibitors as a promising strategy for the treatment of several refractory solid malignancies, including endometrial cancer. Several clinical trials have investigated the efficacy and safety of immune checkpoint inhibitors in endometrial cancer, which already led to the approval of the anti-programmed cell death protein 1 (anti-PD-1) antibody pembrolizumab as a satisfactory alternative for selected patients with unresectable or metastatic disease. As the future of cancer treatment will probably rely on combination therapy strategies, currently, innovative ongoing trials are exploring the potential role of immune checkpoint inhibitors associated with chemotherapy, radiotherapy, and other targeted therapies. Moreover, further research is warranted to discover new specific biomarkers that can accurately predict the response to immunotherapy.
Relationship between Insulin Resistance (HOMA-IR), Trabecular Bone Score (TBS), and Three-Dimensional Dual-Energy X-ray Absorptiometry (3D-DXA) in Non-Diabetic Postmenopausal Women
Authors: Campillo-Sánchez Francisco , Usategui-Martín Ricardo , Ruiz -de Temiño Ángela , Gil Judith , Ruiz-Mambrilla Marta , Fernández-Gómez Jose María , Dueñas-Laita Antonio , Pérez-Castrillón José Luis
Published: June 2020

Background: Insulin may play a key role in bone metabolism, where the anabolic effect predominates. This study aims to analyze the relationship between insulin resistance and bone quality using the trabecular bone score (TBS) and three-dimensional dual-energy X-ray absorptiometry (3D-DXA) in non-diabetic postmenopausal women by determining cortical and trabecular compartments. Methods: A cross-sectional study was conducted in non-diabetic postmenopausal women with suspected or diagnosed osteoporosis. The inclusion criteria were no menstruation for more than 12 months and low bone mass or osteoporosis as defined by DXA. Glucose was calculated using a Hitachi 917 auto-analyzer. Insulin was determined using an enzyme-linked immunosorbent assay (EIA). Insulin resistance was estimated using a homeostasis model assessment of insulin resistance (HOMA-IR). DXA, 3D-DXA, and TBS were thus collected. Moreover, we examined bone parameters according to quartile of insulin, hemoglobin A1C (HbA1c), and HOMA-IR. Results: In this study, we included 381 postmenopausal women. Women located in quartile 4 (Q4) of HOMA-IR had higher values of volumetric bone mineral density (vBMD) but not TBS. The increase was higher in the trabecular compartment (16.4%) than in the cortical compartment (6.4%). Similar results were obtained for insulin. Analysis of the quartiles by HbA1c showed no differences in densitometry values, however women in Q4 had lower levels of TBS. After adjusting for BMI, statistical significance was maintained for TBS, insulin, HOMA-IR, and HbA1c. Conclusions: In non-diabetic postmenopausal women there was a direct relationship between insulin resistance and vBMD, whose effect is directly related to greater weight. TBS had an inverse relationship with HbA1c, insulin, and insulin resistance unrelated to weight. This might be explained by the formation of advanced glycosylation products (AGEs) in the bone matrix, which reduces bone deformation capacity and resistance, as well as increases fragility.
Circulating Tumor DNA as a Preoperative Marker of Recurrence in Patients with Peritoneal Metastases of Colorectal Cancer: A Clinical Feasibility Study
Authors: Beagan Jamie J. , Sluiter Nina R. , Bach Sander , Eijk Paul P. , Vlek Stijn L. , Heideman Daniëlle A. M. , Kusters Miranda , Pegtel D. Michiel , Kazemier Geert , van Grieken Nicole C. T. , Ylstra Bauke , Tuynman Jurriaan B.
Published: June 2020

Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy (CRS-HIPEC) may be curative for colorectal cancer patients with peritoneal metastases (PMs) but it has a high rate of morbidity. Accurate preoperative patient selection is therefore imperative, but is constrained by the limitations of current imaging techniques. In this pilot study, we explored the feasibility of circulating tumor (ct) DNA analysis to select patients for CRS-HIPEC. Thirty patients eligible for CRS-HIPEC provided blood samples preoperatively and during follow-up if the procedure was completed. Targeted Next-Generation Sequencing (NGS) of DNA from PMs was used to identify bespoke mutations that were subsequently tested in corresponding plasma cell-free (cf) DNA samples using droplet digital (dd) PCR. CtDNA was detected preoperatively in cfDNA samples from 33% of patients and was associated with a reduced disease-free survival (DFS) after CRS-HIPEC (median 6.0 months vs median not reached, p = 0.016). This association could indicate the presence of undiagnosed systemic metastases or an increased metastatic potential of the tumors. We demonstrate the feasibility of ctDNA to serve as a preoperative marker of recurrence in patients with PMs of colorectal cancer using a highly sensitive technique. A more appropriate treatment for patients with preoperative ctDNA detection may be systemic chemotherapy in addition to, or instead of, CRS-HIPEC.
Long-Term Changes in Sleep Disordered Breathing in Renal Transplant Patients: Relevance of the BMI
Authors: Mallamaci Francesca , Tripepi Rocco , D’Arrigo Graziella , Porto Gaetana , Versace Maria Carmela , Marino Carmela , Sanguedolce Maria Cristina , Tripepi Giovanni , Zoccali Carmine
Published: June 2020

Sleep disordered breathing (SDB), as defined by the Apnea Hypopnea Index (AHI), is a highly prevalent disturbance in end stage kidney disease. SDB improves early on after renal transplantation but long-term changes in AHI in these patients have not been studied. We studied the long-term changes in AHI in a series of 221 renal transplant patients (mean age: 47 ± 12 years; 70% males) over a median follow up of 35 months. Data analysis was made by the generalized estimating equations method (GEE). On longitudinal observation, the median AHI rose from 1.8 (Interquartile range: 0.6–5.0) to 2.9 (IQR: 1.0–6.6) and to 3.6 (IQR: 1.7–10.4) at the second and third visit, respectively (p = 0.009 by the GEE model and the proportion of patients with moderate to severe SDB rose from 8% to 20%. Longitudinal changes in minimum oxygen saturation (minSaO2) mirrored those in the AHI. In adjusted analyses, repeated measurements of BMI (p < 0.009) emerged as the strongest independent longitudinal correlate of AHI and MinSaO2. The AHI worsens over time in renal transplant patients and longitudinal changes of this biomarker are directly related to simultaneous changes in BMI. Overweight/obesity, a potentially modifiable risk factor, is an important factor underlying the risk of SDB in this population.
Gender Differences in the Levels of Periodontal Destruction, Behavioral Risk Factors and Systemic Oxidative Stress in Ischemic Stroke Patients: A Cohort Pilot Study
Authors: Stănescu Ioana , Bulboacă Adriana Elena , Micu Iulia Cristina , Bolboacă Sorana D. , Feștilă Dana Gabriela , Bulboacă Angelo C. , Bodizs Gyorgy , Dogaru Gabriela , Boarescu Paul Mihai , Popa-Wagner Aurel , Roman Alexandra
Published: June 2020

Background: Due to the higher frequency of ischemic stroke in men compared to women, we aimed to determine if gender differences exist regarding periodontal status and several plasma biomarkers in patients with a recent large artery atherosclerosis ischemic stroke (IS). Material and methods: Patients with their first IS within less than six weeks who were able to undergo periodontal examinations were evaluated. Demographic data, periodontal status, oxidative stress parameters/plasma antioxidant capacity, and C-reactive protein in patients who suffered a recent large artery atherosclerosis ischemic stroke were reccorded. Results: 93 patients were included in the study. More men were smokers (12/57 vs. 3/36) and consumed alcohol (17/57 vs. 3/36), and more women had higher glycemic values (p = 0.023), total cholesterol (p < 0.001), LDL (low-density lipoprotein)-cholesterol (p = 0.010), and HDL (high-density lipoprotein)-cholesterol (p = 0.005) levels. Significantly more men than women had moderate plus severe periodontal disease (p = 0.018), significantly higher levels of nitric oxide (p = 0.034), and significantly lower levels of total antioxidant capacity (p = 0.028). Conclusions: In this pilot study, men seem to be more prone to oxidative stress and to develop more severe forms of periodontitis among patients with stroke, but the results need validation on a larger sample.
(2S,3R,6R)-2-[(R)-1-Hydroxyallyl]-4,4-dimethoxy-6-methyltetrahydro-2H-pyran-3-ol
Authors: Bennett Jack , Murphy Paul
Published: June 2020

(2S,3R,6R)-2-[(R)-1-Hydroxyallyl]-4,4-dimethoxy-6-methyltetrahydro-2H-pyran-3-ol was isolated in 18% after treating the glucose derived (5R,6S,7R)-5,6,7-tris[(triethylsilyl)oxy]nona-1,8-dien-4-one with (1S)-(+)-10-camphorsulfonic acid (CSA). The one-pot formation of the title compound involved triethylsilyl (TES) removal, alkene isomerization, intramolecular conjugate addition and ketal formation. The compound was characterized by 1H and 13C NMR spectroscopy, ESI mass spectrometry and IR spectroscopy. NMR spectroscopy was used to establish the product structure, including the conformation of its tetrahydropyran ring.
Microstructural Characterization and Mechanical Properties of Fiber Laser Welded CP-Ti and Ti-6Al-4V Similar and Dissimilar Joints
Authors: Abdollahi Alireza , Ahnaf Huda Ahmed Shaheer , Kabir Abu Syed
Published: June 2020

In this research, the microstructures and mechanical properties of similar and dissimilar autogenous joints of 3 mm thick commercially pure titanium (CP-Ti) and Ti-6Al-4V welded by ytterbium fiber laser (Yb:YAG) were investigated. Two sets of laser power and welding speed were selected in such a way that the heat input remained constant. Microstructural characterization of the joints was investigated by an optical microscope, and mechanical properties were determined by hardness and tensile tests. The only defects found were porosity and underfill, and no signs of lack of penetration and solidification cracks were observed in any of the joints. Microstructural evaluation of the fusion zone (FZ) showed that in similar Ti-6Al-4V joint, a supersaturated nonequilibrium α′ martensite was formed due to rapid cooling associated with laser welding. In similar CP-Ti, coarse equiaxed grains were observed in the FZ. Unlike the similar joints, a clear interface was observed between the heat-affected zone (HAZ) and the FZ in both the CP-Ti and Ti-6Al-4V sides in dissimilar joints. Among all the joints with different weld parameters, similar Ti-6Al-4V showed the highest strength and the lowest ductility. In similar CP-Ti and dissimilar joints, fractures took place in the CP-Ti base metal, but all the Ti-6Al-4V similar joints failed in the FZ. Significant changes in the strength and hardness with varying laser power and welding speed implied that the mechanical properties of the weld fusion zones were not entirely governed by the heat input but were also affected by individual welding parameters.
Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics
Authors: González-Domínguez Raúl , González-Domínguez Álvaro , Sayago Ana , Fernández-Recamales Ángeles
Published: June 2020

Metabolomics can be significantly influenced by a range of pre-analytical factors, such as sample collection, pre-processing, aliquoting, transport, storage and thawing. This therefore shows the crucial need for standardizing the pre-analytical phase with the aim of minimizing the inter-sample variability driven by these technical issues, as well as for maintaining the metabolic integrity of biological samples to ensure that metabolomic profiles are a direct expression of the in vivo biochemical status. This review article provides an updated literature revision of the most important factors related to sample handling and pre-processing that may affect metabolomics results, particularly focusing on the most commonly investigated biofluids in metabolomics, namely blood plasma/serum and urine. Finally, we also provide some general recommendations and best practices aimed to standardize and accurately report all these pre-analytical aspects in metabolomics research.
Dietary Choices and Habits during COVID-19 Lockdown: Experience from Poland
Authors: Sidor Aleksandra , Rzymski Piotr
Published: June 2020

The outbreak of coronavirus disease (COVID-19) in late December 2019 in China, which later developed into a pandemic, has forced different countries to implement strict sanitary regimes and social distancing measures. Globally, at least four billion people were under lockdown, working remotely, homeschooling children, and facing challenges coping with quarantine and the stressful events. The present cross-sectional online survey of adult Poles (n = 1097), conducted during a nationwide quarantine, aimed to assess whether nutritional and consumer habits have been affected under these conditions. Over 43.0% and nearly 52% reported eating and snacking more, respectively, and these tendencies were more frequent in overweight and obese individuals. Almost 30% and over 18% experienced weight gain (mean ± SD 3.0 ± 1.6 kg) and loss (−2.9 ± 1.5 kg), respectively. Overweight, obese, and older subjects (aged 36–45 and >45) tended to gain weight more frequently, whereas those with underweight tended to lose it further. Increased BMI was associated with less frequent consumption of vegetables, fruit, and legumes during quarantine, and higher adherence to meat, dairy, and fast-foods. An increase in alcohol consumption was seen in 14.6%, with a higher tendency to drink more found among alcohol addicts. Over 45% of smokers experienced a rise in smoking frequency during the quarantine. The study highlights that lockdown imposed to contain an infectious agent may affect eating behaviors and dietary habits, and advocates for organized nutritional support during future epidemic-related quarantines, particularly for the most vulnerable groups, including overweight and obese subjects.
The Role and the Effect of Magnesium in Mental Disorders: A Systematic Review
Authors: Botturi Andrea , Ciappolino Valentina , Delvecchio Giuseppe , Boscutti Andrea , Viscardi Bianca , Brambilla Paolo
Published: June 2020

Introduction: Magnesium is an essential cation involved in many functions within the central nervous system, including transmission and intracellular signal transduction. Several studies have shown its usefulness in neurological and psychiatric diseases. Furthermore, it seems that magnesium levels are lowered in the course of several mental disorders, especially depression. Objectives: In this study, we wish to evaluate the presence of a relationship between the levels of magnesium and the presence of psychiatric pathology as well as the effectiveness of magnesium as a therapeutic supplementation. Methods: A systematic search of scientific records concerning magnesium in psychiatric disorders published from 2010 up to March 2020 was performed. We collected a total of 32 articles: 18 on Depressive Disorders (DD), four on Anxiety Disorders (AD), four on Attention Deficit Hyperactivity Disorder (ADHD), three on Autism Spectrum Disorder (ASD), one on Obsessive–Compulsive Disorder (OCD), one on Schizophrenia (SCZ) and one on Eating Disorders (ED). Results: Twelve studies highlighted mainly positive results in depressive symptoms. Seven showed a significant correlation between reduced plasma magnesium values and depression measured with psychometric scales. Two papers reported improved depressive symptoms after magnesium intake, two in association with antidepressants, compared to controls. No significant association between magnesium serum levels and panic or Generalized Anxiety Disorder (GAD) patients, in two distinct papers, was found. In two other papers, a reduced Hamilton Anxiety Rating Scale (HAM-A) score in depressed patients correlated with higher levels of magnesium and beneficial levels of magnesium in stressed patients was found. Two papers reported low levels of magnesium in association with ADHD. Only one of three papers showed lower levels of magnesium in ASD. ED and SCZ reported a variation in magnesium levels in some aspects of the disease. Conclusion: The results are not univocal, both in terms of the plasma levels and of therapeutic effects. However, from the available evidence, it emerged that supplementation with magnesium could be beneficial. Therefore, it is necessary to design ad hoc clinical trials to evaluate the efficacy of magnesium alone or together with other drugs (antidepressants) in order to establish the correct use of this cation with potential therapeutic effects.
Lassa Fever: Viral Replication, Disease Pathogenesis, and Host Immune Modulations
Authors: Ly Hinh
Published: June 2020

Despite major discoveries made in the last few decades about Lassa fever, there are still many unresolved key issues that hamper the development of effective vaccines and therapies against this deadly disease that is endemic in several West African countries. Some of these issues include the lack of a detailed understanding of the viral and participating host factors in completing the virus life cycle, in mediating disease pathogenesis or protection from disease, and in activating or suppressing host innate and cellular immunity against virus infection, as well as of the animal models required for testing vaccines and therapeutics. This Special Issue is devoted to understanding some of these important issues and to exploring the current status of the research and development in combating Lassa fever.
Editorial Comments to the Special Issue: “Aggregatibacter actinomycetemcomitans—Gram-Negative Bacterial Pathogen”
Authors: Oscarsson Jan , DiRienzo Joseph , Johansson Anders
Published: June 2020

Aggregatibacter actinomycetemcomitans is a periodontal pathogen colonizing the oral cavity in many individuals of the human population. It is equipped with several potent virulence factors that can cause cell death and induce or evade the host inflammatory response. Both harmless and highly virulent genotypes of the bacterium have emerged because of the large genetic diversity within the species. The oral condition and age, as well as the geographic origin of the individual, influence the risk to be colonized by a virulent genotype of the bacterium. In the present editorial, the different genetic and virulence properties of A. actinomycetemcomitans will be addressed in relation to the publications in this Special Issue.
Genetic Structure of Wild Germplasm of Macadamia: Species Assignment, Diversity and Phylogeographic Relationships
Authors: Mai Thuy , Alam Mobashwer , Hardner Craig , Henry Robert , Topp Bruce
Published: June 2020

Macadamia is an Australian native rainforest tree that has been domesticated and traded internationally for its premium nuts. Common cultivars rely upon a limited gene pool that has exploited only two of the four species. Introducing a more diverse germplasm will broaden the genetic base for future crop improvement and better adaptation for changing environments. This study investigated the genetic structure of 302 accessions of wild germplasm using 2872 SNP and 8415 silicoDArT markers. Structure analysis and principal coordinate analysis (PCoA) assigned the 302 accessions into four distinct groups: (i) Macadamia integrifolia, (ii) M. tetraphylla, and (iii) M. jansenii and M. ternifolia, and (iv) admixtures or hybrids. Assignment of the four species matched well with previous characterisations, except for one M. integrifolia and four M. tetraphylla accessions. Using SNP markers, 94 previously unidentified accessions were assigned into the four distinct groups. Finally, 287 accessions were identified as pure examples of one of the four species and 15 as hybrids of M. integrifolia and M. tetraphylla. The admixed accessions showed the highest genetic diversity followed by M. integrifolia, while M. ternifolia and M. jansenii accessions were the least diverse. Mantel test analysis showed a significant correlation between genetic and geographic distance for M. integrifolia (r = 0.51, p = 0.05) and a positive but not significant correlation for M. tetraphylla (r = 0.45, p = 0.06). This study provides a population genetics overview of macadamia germplasm as a background for a conservation strategy and provides directions for future macadamia breeding.
The Efficacy of Polymer Coatings for the Protection of Electroless Copper Plated Polyester Fabric
Authors: Ojstršek Alenka , Virant Natalija , Fox Daryl , Krishnan Latha , Cobley Andrew
Published: June 2020

The selective metallisation of textiles is becoming a very important process in the development of electronic or e-textiles. This study investigated the efficacy of polymer coatings for the protection of copper (Cu) conductive tracks electroless plated on polyester (PES) fabric against laundering and rubbing, without essentially affecting the physical-mechanical and optical properties of the base material. After the electroless deposition of a consistent layer of Cu onto PES, four polymers were applied individually by screen-printing or padding. The physical-mechanical characterisation of coated textiles revealed that polyurethane resin (PUR) and modified acrylate resin (AR) had little effect on the air permeability, tensile strength and breaking tenacity of the PES, as compared to silicone elastomer polydimethylsiloxane (PDMS) and epoxy resin (ER). On the other hand, PUR and PDMS had higher abrasion resistance and photo-stability under prolonged UV irradiation, as compared to AR and ER. In addition, freshly Cu plated samples were coated with polymers, washed up to 30 cycles and characterised by measuring their electrical resistivity, determination of colour changes and the examination of the surface morphology. Based on these results, PUR presented the most suitable protection of Cu tracks on PES, with the lowest impact on physical-mechanical properties. ER is not recommended to be used for protection of Cu tracks on fabrics, due to its rigidity, low photo-stability, washing and wear durability.
Dynamics of Dalk Glacier in East Antarctica Derived from Multisource Satellite Observations Since 2000
Authors: Chen Yiming , Zhou Chunxia , Ai Songtao , Liang Qi , Zheng Lei , Liu Ruixi , Lei Haobo
Published: June 2020

Monitoring variability in outlet glaciers can improve the understanding of feedbacks associated with calving, ocean thermal forcing, and climate change. In this study, we present a remote-sensing investigation of Dalk Glacier in East Antarctica to analyze its dynamic changes. Terminus positions and surface ice velocities were estimated from Landsat and Sentinel-1 data, and the high-precision Worldview digital elevation model (DEM) was generated to determine the location of the potential ice rumple. We detected the cyclic behavior of glacier terminus changes and similar periodic increases in surface velocity since 2000. The terminus retreated in 2006, 2009, 2010, and 2016 and advanced in other years. The surface velocity of Dalk Glacier has a 5-year cycle with interannual speed-ups in 2007, 2012, and 2017. Our observations show the relationship between velocity changes and terminus variations, as well as the driving role of the ice rumple. Ice velocity often increases after calving events and continuous retreats. The loss of buttressing provided by an ice rumple may be a primary factor for increases in ice velocity. Given the restriction of the ice rumple, the surface velocity remains relatively stable when the glacier advances. The calving events may be linked to the unstable terminus caused by the ice rumple.
Geosynchronous Spaceborne-Airborne Bistatic Moving Target Indication System: Performance Analysis and Configuration Design
Authors: Dong Xichao , Cui Chang , Li Yuanhao , Hu Cheng
Published: June 2020

Geosynchronous spaceborne-airborne bistatic synthetic aperture radar (GEO SA-BSAR), consisting of GEO transmitter and airborne receiver, has stable coverage for a long time and benefits moving target detection. However, the performance of GEO SA-BSAR moving target indication (MTI) system varies widely between bistatic configurations. The traditional configuration design for GEO SA-BSAR system only considers the imaging performance, which may cause the poor MTI performance. In this paper, we propose a bistatic configuration design method to jointly optimize the MTI and SAR imaging performance for GEO SA-BSAR MTI system. The relationship between the MTI performance and bistatic configuration parameters is derived analytically and analyzed based on the maximum output signal to clutter and noise ratio (SCNR) criterion. Then, the MTI performance and SAR imaging performance are jointly considered to model the configuration design problem as a multi-objective optimization problem under the constrained condition. Finally, the optimal configuration for GEO SA-BSAR MTI system is given.
A Case Study on Microphysical Characteristics of Mesoscale Convective System Using Generalized DSD Parameters Retrieved from Dual-Polarimetric Radar Observations
Authors: Kwon Soohyun , Jung Sung-Hwa , Lee GyuWon
Published: June 2020

The microphysical characteristics of a mesoscale convective system (MCS) during a summer monsoon of South Korea are investigated using the generalized drop size distributions (DSD) that are derived from S-band dual-polarization radar data. The characteristics parameters of generalized DSDs (generalized number concentration, N0′ and generalized mean diameter, Dm) are directly calculated from DSD’s two moments without any assumption on the DSD model. Relationships between ZDR and generalized DSD parameters normalized by ZH are derived in the form of the polynomial equation. Verification of the retrieved DSD parameters is conducted with the 2-D video disdrometer (2DVD) located about 23 km from the radar. The standard deviations (SD) of retrieved DSD parameters are about 0.26 for log N0′, and about 0.11 for Dm because of the variability of DSDs. The SD of the retrieved log N0′ from the dual-polarimetric measurement reaches to about 0.46 (almost double) for 11 rain events while the accuracy of retrieved Dm is quite higher (~0.19). This higher error in retrieved log N0′ is likely attributed to the larger discrepancy in radar-observed and DSD-calculated ZDR when ZH is low. This retrieval technique is applied to a mesoscale convective system (MCS) case to investigate the Lagrangian characteristics of the microphysical process. The MCS is classified into the leading edge and trailing stratiform region by using the storm classification algorithm. The leading edge dominated by strong updraft showed the broad DSD spectra with a steady temporal increase of Dm throughout the event, likely because of the dominant drop growth by the collision-coalescence process. On the other hand, the drop growth is less significant in the trailing stratiform region as shown by the nearly constant Dm for the entire period. The DSD variation is also controlled by the new generation of drops in the leading edge and less extent in the trailing stratiform during the early period when precipitation systems grow. When the system weakens, the characteristic number concentration decreases with time, indicating the new generation of drops becomes less significant in both regions.