bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing vulnerability and enhancing resilience of port systems in southeast Texas facing sea-level rise
Authors: Amer Reda
Published: May 2024

Climate change and the associated sea level rise (SLR) are presenting newfound challenges to the port systems and coastal transportation infrastructure of southeast Texas. This paper introduces a Geographic Information Systems (GIS) based model designed to simulate inundation scenarios under various sea-level projections, aiming to assess the vulnerabilities of both port facilities and road networks. The study area encompasses a specific region within Jefferson County, southeast Texas, encompassing three major ports: Port Arthur, Beaumont, and Orange. Utilizing a high-resolution (1-m) Digital Elevation Model (DEM) derived from the 2017 LiDAR dataset, this model is integrated with NASA’s sea-level rise projections to compute the extent and volume of inundation across low, medium, and high SLR scenarios. Drawing from monthly mean sea level data spanning from 1958 to 2020, the lowest SLR projections, derived from the relative sea-level trend measured at the Sabine Pass, TX gauge station, indicate a yearly increase of 6.16 mm, with a 95% confidence interval of +/- 0.74 mm. Projections for 2050 and 2,100 show the lowest SLR at 0.17 m and 0.48 m, respectively. In contrast, the medium to high RSLR projections under the IPCC SSP3-7.0 scenario for 2050 and 2,100 stand at 0.54 m and 1.34 m, respectively. The findings reveal that, under medium to high SLR scenarios, the extent of inundated areas in the study region is expected to expand by 12.4% in 2050 and 19.9% in 2,100, compared to the lowest SLR projection. Additionally, the length of submerged roadways is predicted to increase by 6.9% in 2050 and 13.3% in 2,100, in comparison to the lowest SLR projection. It is worth noting that some margin of error may be introduced due to factors such as the width of the port area and access roads, the high-resolution DEM, and the alignment of computed inundated areas with the existing topography. Overall, the manuscript highlights the urgency of proactive planning and underscores the importance of safeguarding critical infrastructure in the context of climate change and SLR.
Functional role of autophagy in testicular and ovarian steroidogenesis
Authors: Afzal Ali , Zhang Yue , Afzal Hanan , Saddozai Umair Ali Khan , Zhang Lei , Ji Xin-Ying , Khawar Muhammad Babar
Published: May 2024

Autophagy is an evolutionarily conserved cellular recycling process that maintains cellular homeostasis. Despite extensive research in endocrine contexts, the role of autophagy in ovarian and testicular steroidogenesis remains elusive. The significant role of autophagy in testosterone production suggests potential treatments for conditions like oligospermia and azoospermia. Further, influence of autophagy in folliculogenesis, ovulation, and luteal development emphasizes its importance for improved fertility and reproductive health. Thus, investigating autophagy in gonadal cells is clinically significant. Understanding these processes could transform treatments for endocrine disorders, enhancing reproductive health and longevity. Herein, we provide the functional role of autophagy in testicular and ovarian steroidogenesis to date, highlighting its modulation in testicular steroidogenesis and its impact on hormone synthesis, follicle development, and fertility therapies.
Plasmodium vivax spleen-dependent protein 1 and its role in extracellular vesicles-mediated intrasplenic infections
Authors: Ayllon-Hermida Alberto , Nicolau-Fernandez Marc , Larrinaga Ane M. , Aparici-Herraiz Iris , Tintó-Font Elisabet , Llorà-Batlle Oriol , Orban Agnes , Yasnot María Fernanda , Graupera Mariona , Esteller Manel , Popovici Jean , Cortés Alfred , del Portillo Hernando A. , Fernandez-Becerra Carmen
Published: May 2024

Recent studies indicate that human spleen contains over 95% of the total parasite biomass during chronic asymptomatic infections caused by Plasmodium vivax. Previous studies have demonstrated that extracellular vesicles (EVs) secreted from infected reticulocytes facilitate binding to human spleen fibroblasts (hSFs) and identified parasite genes whose expression was dependent on an intact spleen. Here, we characterize the P. vivax spleen-dependent hypothetical gene (PVX_114580). Using CRISPR/Cas9, PVX_114580 was integrated into P. falciparum 3D7 genome and expressed during asexual stages. Immunofluorescence analysis demonstrated that the protein, which we named P. vivax Spleen-Dependent Protein 1 (PvSDP1), was located at the surface of infected red blood cells in the transgenic line and this localization was later confirmed in natural infections. Plasma-derived EVs from P. vivax-infected individuals (PvEVs) significantly increased cytoadherence of 3D7_PvSDP1 transgenic line to hSFs and this binding was inhibited by anti-PvSDP1 antibodies. Single-cell RNAseq of PvEVs-treated hSFs revealed increased expression of adhesion-related genes. These findings demonstrate the importance of parasite spleen-dependent genes and EVs from natural infections in the formation of intrasplenic niches in P. vivax, a major challenge for malaria elimination.
Six factors facilitating teachers’ use of research. An experimental factorial survey of educational stakeholders perspectives
Authors: Jeune Nathanael , Juhel Jacques , Dessus Philippe , Atal Ignacio
Published: May 2024

Introduction

Teachers’ use of research has been increasingly advocated for in the past few decades, and some research has documented the factors which positively or negatively influence teachers’ use of research. However, the existing research does not give relevant information to prioritize between different ways to facilitate teachers’ use of research. In addition, different professionals working in education may have divergent opinions about such priorities. This study therefore asks what are the factors that most influence teachers’ use of research according to teachers, teacher trainers, educational decision makers and researchers?

Methods

We conducted a factorial survey experiment on six factors with 100 participants (pilot study) and 340 participants (main study) to identify which factors were perceived as influencing the most teachers’ use of research and to compare respondents’ perceptions according to their main role in education.

Results

This study shows that support for research use by the institution and instrumental utility of research are the factors that were judged as most impactful. Some categories of respondents had conflicting views about specific factors, for instance researchers perceiving teachers’ involvement in research as less likely to facilitate teachers’ use of research.

Conclusion

These findings can help decision-makers and teacher-trainers with limited resources to allocate them in a more effective way, while taking into consideration the disagreements across professions in order to resolve possibly arising conflicts.
On the importance of varying device thickness and temperature on the outcome of space-charge-limited current measurements
Authors: Zhao Alfred , Le Corre Vincent M. , Röhr Jason A.
Published: May 2024

Space-charge-limited current (SCLC) measurements are commonly employed to characterize charge-transport properties of semiconductors used in next-generation thin-film optoelectronics, such as organic π-conjugated small molecules and polymers, and metal-halide perovskites. Despite the wide-spread adoption of the method, there is no community-wide consensus around how SCLC measurements should be performed, nor how the data should be analyzed and reported. While it is common to report device characteristics by employing a simplistic analytical model for fitting a single J-V curve obtained from a solitary device at room temperature—sometimes in a very select voltage range—expectedly, such an approach will often not give an accurate picture of the underlying physics. On that account, we here aim to highlight the importance of reporting values extracted from not just a solitary single-carrier device measured at room temperature, but from devices with different thicknesses measured at varying device temperature. We also highlight how the choice of device thickness is especially critical in determining what device and material characteristics can be extracted from SCLC measurements, and how this choice can greatly affect the conclusions drawn about the probed semiconducting material. While other factors could affect the outcome of an SCLC measurement and the subsequent analysis, we hope that the topics covered in this article will result in overall improved charge-transport characterization of thin-film semiconductors and initiate a broader discussion into SCLC metrology at large.
Ketogenic diet enhances the anti-cancer effects of PD-L1 blockade in renal cell carcinoma
Authors: Richard Jeremy , Beauvillain Céline , Benoit Maxime , Barth Magalie , Aubert Cécile , Rolley Cyrielle , Bellal Sarah , Bourreau Jennifer , Ferragu Matthieu , Lebdai Souhil , Chevrollier Arnaud , Henrion Daniel , Procaccio Vincent , Bigot Pierre
Published: May 2024

Introduction

Clear cell renal cell carcinoma (ccRCC) is characterized by a predominant metabolic reprogramming triggering energy production by anaerobic glycolysis at the expense of oxydative phosphorylation. Ketogenic diet (KD), which consists of high fat and low carbohydrate intake, could bring required energy substrates to healthy cells while depriving tumor cells of glucose. Our objective was to evaluate the effect of KD on renal cancer cell tumor metabolism and growth proliferation.

Methods

Growth cell proliferation and mitochondrial metabolism of ACHN and Renca renal carcinoma cells were evaluated under ketone bodies (KB) exposure. In vivo studies were performed with mice (nude or Balb/c) receiving a xenograft of ACHN cells or Renca cells, respectively, and were then split into 2 feeding groups, fed either with standard diet or a 2:1 KD ad libitum. To test the effect of KD associated to immunotherapy, Balb/c mice were treated with anti-PDL1 mAb. Tumor growth was monitored.

Results

In vitro, KB exposure was associated with a significant reduction of ACHN and Renca cell proliferation and viability, while increasing mitochondrial metabolism. In mice, KD was associated with tumor growth reduction and PDL-1 gene expression up-regulation. In Balb/c mice adjuvant KD was associated to a better response to anti-PDL-1 mAb treatment.

Conclusion

KB reduced the renal tumor cell growth proliferation and improved mitochondrial respiration and biogenesis. KD also slowed down tumor growth of ACHN and Renca in vivo. We observed that PDL-1 was significantly overexpressed in tumor in mice under KD. Response to anti-PDL-1 mAb was improved in mice under KD. Further studies are needed to confirm the therapeutic benefit of adjuvant KD combined with immunotherapy in patients with kidney cancer.
Effects of long and short ejaculatory abstinence on sperm parameters: a meta-analysis of randomized-controlled trials
Authors: Lo Giudice Arturo , Asmundo Maria Giovanna , Cimino Sebastiano , Cocci Andrea , Falcone Marco , Capece Marco , Abdelhameed Ali Saber , Capogrosso Paolo , Morgado Afonso , Tsampoukas Georgios , Manfredi Celeste , Russo Giorgio Ivan
Published: May 2024

Purpose

This study aimed to investigate the effects of ejaculatory abstinence on sperm parameters.

Methods

This analysis was registered in PROSPERO (CRD42023472124). We performed a search on PubMed using the following text terms: ((“sperm parameters” OR “sperm analysis” [Mesh]) AND (“sperm DNA fragmentation” OR “DNA fragmentation” [Mesh]) AND (“sexual abstinence” [Mesh] OR “abstinence”)) and an advanced search in Scopus using the terms (“sperm parameters” OR “sperm parameters” OR “DNA fragmentation”) AND (“abstinence”). The sperm parameters that were investigated were sperm volume, total sperm motility, progressive sperm motility, sperm concentration, sperm morphology, and sperm DNA fragmentation (SDF). A two-day cut-off as a “short” or “long” abstinence period has been defined.

Results

Thirteen studies published between 2013 and 2022 were included in this meta-analysis. A total of 2,315 patients, ranging from 6 to 836 from each cohort, were enrolled in the study. We showed that longer abstinence time was associated with greater sperm concentration (mean difference [MD]: 8.19; p <0.01), sperm volume (MD: 0.96; p <0.01), and higher SDF (MD: 3.46; p <0.01), but lower progressive sperm motility (MD: −1.83; p <0.01). Otherwise, no statistically significant difference was observed in patients with longer vs. shorter abstinence times regarding total sperm motility (MD: −1.83; p = 0.06). Meta-regression analysis showed that days of abstinence were positively and linearly related to sperm concentration (slope: 3.74; p <0.01) and SDF (slope: 0.65; p = 0.044).

Conclusions

According to our data, short ejaculatory abstinence is associated with better sperm quality. Indeed, a higher percentage of progressive sperm motility and lower levels of SDF have been reported in a short abstinence cohort. In contrast, the long abstinence group reported a higher sperm concentration.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42023472124.
Identification of cytokines in benign and malignant thymus tumors: based on Mendelian randomization and proteomics
Authors: Zhu Kangle , Shi Jingwei , Yang Rusong , Zhou Chu , Liu Zhengcheng
Published: May 2024

Objective

The aim of this study was to identify potential causal cytokines in thymic malignancies and benign tumors from the FinnGen database using Mendelian randomization (MR).

Methods

In this study, data from genome-wide association studies (GWAS) of 91 cytokines were used as exposure factors, and those of thymic malignant tumors and thymic benign tumors were the outcome variables. Two methods were used to determine the causal relationship between exposure factors and outcome variables: inverse variance weighting (IVW) and MR-Egger regression. Sensitivity analysis was performed using three methods, namely, the heterogeneity test, the pleiotropy test, and the leave-one-out test.

Results

There was a causal relationship between the expression of fibroblast growth factor 5, which is a risk factor for thymic malignant tumors, and thymic malignant tumors. C-C motif chemokine 19 expression, T-cell surface glycoprotein CD5 levels, and interleukin-12 subunit beta levels were causally related to thymic malignant tumors and were protective. Adenosine deaminase levels, interleukin-10 receptor subunit beta expression, tumor necrosis factor (TNF)-related apoptosis-inducing ligand levels, and TNF-related activation-induced cytokine levels showed a causal relationship with thymic benign tumors, which are its risk factors. Caspase 8 levels, C-C motif chemokine 28 levels, interleukin-12 subunit beta levels, latency-associated peptide transforming growth factor beta 1 levels, and programmed cell death 1 ligand 1 expression showed a causal relationship with thymic benign tumors, which are protective factors. Sensitivity analysis showed no heterogeneity.

Conclusion

Cytokines showed a causal relationship with benign and malignant thymic tumors. Interleukin-12 subunit beta is a common cytokine that affects malignant and benign thymic tumors.
Complex fracture description and quasi-elastic energy development mathematical model for shale oil reservoirs
Authors: Rui Wang , Zhengdong Lei , Han Jia , Yang Yang , Yiqun Yan
Published: May 2024

Reservoir numerical simulation is an important tool and method for the reasonable and efficient development of shale reservoirs. Accurate description of three-dimensional fractures in shale reservoir development is a necessary and sufficient condition to improve the accuracy and robustness of shale reservoir numerical simulation. This paper achieves precise characterization of complex fracture shapes and oil, gas and water flow by establishing an embedded discrete fracture model based on a non-structural network, which has advantages in the fine characterization of complex morphological fractures in the reservoir and the grid division of the reservoir. In the large matrix solution method, the Newton-Raphson method is used to linearize the nonlinear equations, the Jacobian matrix is ​​constructed, the ILU method is used for preprocessing, the conjugate gradient method is used to solve the linear equations, and the shale oil quasi-elasticity is established A fully implicit solution method for mathematical models of energy development.
Genetic determinants of serum bilirubin using inferred native American gene variants in Chilean adolescents
Authors: Miranda José P. , Pereira Ana , Corvalán Camila , Miquel Juan F. , Alberti Gigliola , Gana Juan C. , Santos José L.
Published: May 2024

Gene variants in the UGT1A1 gene are strongly associated with circulating bilirubin levels in several populations, as well as other variants of modest effect across the genome. However, the effects of such variants are unknown regarding the Native American ancestry of the admixed Latino population. Our objective was to assess the Native American genetic determinants of serum bilirubin in Chilean admixed adolescents using the local ancestry deconvolution approach. We measured total serum bilirubin levels in 707 adolescents of the Chilean Growth and Obesity Cohort Study (GOCS) and performed high-density genotyping using the Illumina-MEGA array (>1.7 million genotypes). We constructed a local ancestry reference panel with participants from the 1000 Genomes Project, the Human Genome Diversity Project, and our GOCS cohort. Then, we inferred and isolated haplotype tracts of Native American, European, or African origin to perform genome-wide association studies. In the whole cohort, the rs887829 variant and others near UGT1A1 were the unique signals achieving genome-wide statistical significance (b = 0.30; p = 3.34 × 10−57). After applying deconvolution methods, we found that significance is also maintained in Native American (b = 0.35; p = 3.29 × 10−17) and European (b = 0.28; p = 1.14 × 10−23) ancestry components. The rs887829 variant explained a higher percentage of the variance of bilirubin in the Native American (37.6%) compared to European ancestry (28.4%). In Native American ancestry, carriers of the TT genotype of this variant averaged 4-fold higher bilirubinemia compared to the CC genotype (p = 2.82 × 10−12). We showed for the first time that UGT1A1 variants are the primary determinant of bilirubin levels in Native American ancestry, confirming its pan-ethnic relevance. Our study illustrates the general value of the local ancestry deconvolution approach to assessing isolated ancestry effects in admixed populations.
Chasing colonization back: rethinking parks, returning place-names, and restoring buffalo medicine—an interview with Ninna Piiksii, Dr. Mike Bruised Head
Authors: Piiksii Ninna , Lunstrum Elizabeth , Stevens Madison
Published: May 2024

In this interview, we hear from influential Blackfoot Elder and Cultural Educator Ninna Piiksii, Dr. Michael Bruised Head. Mike reflects on the colonial naming of national parks and the need to return to Indigenous place-names, examining how we occupy a pivotal moment where park staff are more open to substantive Indigenous engagement and presence within parks, although more needs to be done. Drawing connections across topics that may initially seem discrete, Mike reflects on his experience as a survivor of the Canadian residential school system, colonial dispossession by parks and more broadly, and how Blackfoot restoration efforts—including the return of buffalo or iinnii—can offer paths for healing from these traumas and build a more just, Blackfoot-led future. Through this, Mike asks us to rethink the profound value and potential of conservation, pushing beyond Western understandings. He closes by asking the interviewers to reflect on what motivates them to support Tribal buffalo restoration, turning the tables on the interviewer and interviewee, and reinforcing the importance of connection and responsibility among non-Tribal research collaborators. We open with an introduction to Mike and then turn to hear his words. The interview format reflects a growing trend of expert-interviews-as-articles and Indigenous practices of oral knowledge transmission. We also link to an audio recording of the interview to allow readers to become listeners and hear Mike’s words in full context. The conversation and format are offered in the spirit of opening more space for Indigenous—and particularly Blackfoot—voices, perspectives, and methodologies in conservation scholarship.
Vaccine hesitancy in patients presenting to a specialized allergy center: clinical relevant sensitizations, impact on mental health and vaccination rates
Authors: Kogseder Natalie , Puxkandl Viktoria , Hoetzenecker Wolfram , Altrichter Sabine
Published: May 2024

Introduction

The COVID vaccination program with new types of vaccinations and early reports of allergic reactions to vaccines led to vaccination hesitancy in patients with allergies. In this study, we aimed to characterize patients who present at an allergy center with specific questions regarding risk assessment to COVID vaccines in comparison to regular allergy center patients.

Methods

A total of 50 patient charts of patients with risk assessment for COVID vaccination (COV group) and 50 regular allergy center patients (ALL group) were assessed for documented allergies, comorbidities, total IgE, and tryptase levels and hospital anxiety and depression score (HADS). Skin prick testing (SPT) with additives of COVID vaccines [polyethylene glycol (PEG), polysorbate] were performed if indicated based on medical history.

Results

Patients who presented for examination prior to a possible COVID vaccination were mostly female (86%) and had more frequently reported allergic reactions to drugs in the past, but only in a minor group (28%) were the reactions qualified as anaphylaxis. The group COV patients scored significantly higher in the HADS for anxiety and depression than the regular group ALL patients. The same trend was observed when data were corrected for gender. It is worth noting that patients without any prior contact to COVID vaccines scored comparable regarding anxiety to patients with prior reaction to COVID vaccinations, but significantly higher in the depression score. In 19 patients (38%) who met the indications for SPT for the suspicious contents PEG and Polysorbate 80, the tests did not show a positive result. Furthermore, 84% of patients underwent the prick test, but only 15% of patients who received consultation alone agreed to vaccination at our center. No vaccination-related event was documented in these patients.

Discussion

In conclusion, vaccination hesitancy was frequently elicited by negative experiences with drugs and putative drug allergies. Female patients predominate in this patient group, and the anxiety and depression scores were significantly elevated. Allergological workup, including SPT, led to a high rate of subsequent vaccinations, whereas a discussion with the patients about risks and individualized advice for vaccination without testing only rarely resulted in documented vaccinations.
Extracellular vescicles in psoriasis: from pathogenesis to possible roles in therapy
Authors: Iuliano Marco , Grimaldi Lorenzo , Rosa Paolo , Scibetta Sofia , Bernardini Nicoletta , Proietti Ilaria , Tolino Ersilia , Skroza Nevena , Potenza Concetta , Mangino Giorgio , Romeo Giovanna
Published: May 2024

Psoriasis is a chronic inflammatory disease affecting skin and joints characterized by a chronically altered immune and inflammatory response. Several factors occur from the onset to the development of this disease due to different types of cells spatially and temporally localized in the affected area, such as, keratinocytes, macrophages, neutrophils and T helper lymphocytes. This scenario leads to the chronic release of high levels of inflammatory mediators (i.e., IL-17, IL-23, IL-22, TNF-α, S100 proteins, Defensins) and lastly parakeratosis and thickening of the stratum spinosum. Extracellular vesicles (EVs) are small double membraned biological nanoparticles that are secreted by all cell types and classified, based on dimension and biogenesis, into exosomes, microvesicles and apoptotic bodies. Their role as vessels for long range molecular signals renders them key elements in the pathogenesis of psoriasis, as well as innovative platforms for potential biomarker discovery and delivery of fine-tuned anti-inflammatory therapies. In this review, the role of EVs in the pathogenesis of psoriasis and the modulation of cellular microenvironment has been summarized. The biotechnological implementation of EVs for therapy and research for new biomarkers has been also discussed.
Estimation of geoacoustic parameters and source range using airgun sounds in the East Siberian Sea, Arctic Ocean
Authors: Lee Dae Hyeok , Han Dong-Gyun , Choi Jee Woong , Son Wuju , Yang Eun Jin , La Hyoung Sul , Tang Dajun
Published: May 2024

Dispersion is a representative property of low-frequency sound propagation over long distances in shallow-water waveguides, making dispersion curves valuable for geoacoustic inversion. This study focuses on estimating the geoacoustic parameters using the dispersion curves extracted from airgun sounds received in the East Siberian Sea. The seismic survey was conducted in September 2019 by the icebreaking research vessel R/V Araon, operated by the Korea Polar Research Institute. A single hydrophone was moored at the East Siberian Shelf, characterized by nearly range-independent shallow water (<70 m) with a hard bottom. In the spectrogram of the received sounds, the dispersion curves of the first two modes were clearly observed. Utilizing a combination of warping transform and wavelet synchrosqueezing transform these two modes were separated. Then, the geoacoustic parameters, such as sound speed and density in the sediment layer, were estimated by comparing the two modal curves extracted at a source-receiver distance of approximately 18.6 km with the predictions obtained by the KRAKEN normal-mode propagation model. Subsequently, the distances between the airgun and the receiver system in the 18.6 to 121.5 km range were estimated through the comparison between the measured modal curves and the model replicas predicted using the estimated geoacoustic parameters.
The location of Tartessos: a fresh case for the reliability of Avienus’ Ora Maritima
Authors: Villarías-Robles Juan J. R. , Rodríguez-Ramírez Antonio , López-Sáez José Antonio , Celestino-Pérez Sebastián , León Ángel
Published: May 2024

Recent geomorphological, paleoenvironmental, and chronological evidence allows for a reconstruction of the coasts of southwest Iberia during the first millennium BCE that accords with descriptions of the region offered by authors of antiquity, most notably the description by the fourth-century CE Roman writer R. F. Avienus in his poem Ora Maritima. This poem contains information that appears to date from the sixth century BCE regarding, for instance, the pre-Roman polity of Tartessos. The reliability of this work as a historical source has been questioned for decades. Critics argue that the information is pertinent to our understanding of the literary, rather than historiographical, context of the Late Roman Empire. However, philological as well as historical analyses reveal no clear cause to doubt the documentary value of the Ora Maritima. Furthermore, geomorphological research makes it possible to identify most place-names in the poem; for example, the city embraced by the river Tartessos, apparently the political and trade center of the realm, may likely have stood on the present-day spit of La Algaida, which was an isle in the first millennium BCE. While this hypothesis has been advanced elsewhere, this article offers an entirely new set of evidences to support it.1
Uncovering sarcopenia and frailty in older adults by using muscle ultrasound—A narrative review
Authors: Prell Tino , Grimm Alexander , Axer Hubertus
Published: May 2024

Muscle ultrasound is a valuable non-invasive and cost-effective method in assessing muscle mass and structure, both of which are significant indicators for the development of sarcopenia and frailty in elderly individuals. Sarcopenia refers to the loss of muscle mass and strength that occurs with age, whereas frailty is a complex geriatric syndrome characterized by reduced physical function and an increased susceptibility to negative health outcomes. Both conditions are prevalent in older adults and are associated with higher risks of falls, disability, and mortality. By measuring muscle size and structure and several other ultrasound parameters, including muscle thickness, cross-sectional area, echogenicity (brightness in the ultrasound image), pennation angle, and fascicle length ultrasound can assist in identifying sarcopenia and frailty in older adults. In addition, ultrasound can be used to evaluate muscle function such as muscle contraction and stiffness, which may also be affected in sarcopenia and frailty. Therefore, muscle ultrasound could lead to better identification and tracking of sarcopenia and frailty. Such advancements could result in the implementation of earlier interventions to prevent or treat these conditions, resulting in an overall improvement in the health and quality of life of the elderly population. This narrative review describes the benefits and challenges when using ultra-sound for the evaluation of frailty and sarcopenia.
Mendelian randomization analysis reveals causal relationships between circulating cell traits and renal disorders
Authors: Shi Xing-yu , Zhang Qian-kun , Li Jie , Zhu Chao-yong , Jin Lie , Fan Shipei
Published: May 2024

Purpose

The aim of this study was to investigate the causal relationships between circulating cell traits and risk of renal disorders.

Methods

We applied a comprehensive two-sample Mendelian randomization (MR) analysis. Single nucleotide polymorphisms (SNPs) from publicly available genome-wide association studies (GWAS) databases were utilized. Genetically predicted instrumental variables of human blood cell traits were extracted from Blood Cell Consortium (BCX) while data on renal diseases was obtained from Finngen consortium. The primary MR analysis was conducted using the inverse variance weighted (IVW) method, with the weighted median (WM) and MR-Egger models used as additional methods. Sensitivity analyses, including MR-PRESSO, radial regression and MR-Egger intercept were conducted to detect outliers and assess horizontal pleiotropy. We further utilized the leave-one-out analysis to assess the robustness of the results. Causal associations were considered significant based on false rate correction (FDR), specifically when the IVW method provided a pFDR < 0.05.

Results

Our results demonstrated that both white blood cell (WBC) count (OR = 1.50, 95% CI = 1.10–2.06, pFDR = 0.033, pIVW = 0.011) and lymphocyte count (OR = 1.50, 95% CI = 1.13–1.98, pFDR = 0.027, pIVW = 0.005) were causally associated with a higher risk of IgA nephropathy. Furthermore, WBC count was identified as a significant genetic risk factor for renal malignant neoplasms (OR = 1.23, 95% CI = 1.06–1.43, pFDR = 0.041, pIVW = 0.007). Additionally, an increased level of genetically predicted eosinophils was found to be causally associated with a higher risk of diabetic nephropathy (OR = 1.21, 95% CI = 1.08–1.36, pFDR = 0.007, pIVW = 0.001). No evidence of pleiotropy was determined.

Conclusion

Our findings provide evidence of causal associations of circulating WBC count, lymphocyte count and IgA nephropathy, WBC count and renal malignant neoplasms, and eosinophil count and diabetic nephropathy. These results have the potential to contribute to the development of novel diagnostic options and therapeutic strategies for renal disorders.
Current treatment of biliary Sphincter of Oddi Dysfunction
Authors: Zeng Hong-Ze , Yi Hang , He Song , Wu Rong , Ning Bo
Published: May 2024

The sphincter of Oddi is a delicate neuromuscular structure located at the junction of the biliary-pancreatic system and the duodenum. Sphincter of Oddi Dysfunction (SOD) can result in various clinical manifestations, including biliary-type pain and recurrent idiopathic pancreatitis. The management of SOD has been challenging. With the publication of the landmark Evaluating Predictors and Interventions in Sphincter of Oddi Dysfunction (EPISOD) trial and the Rome IV consensus, our clinical practice in the treatment of SOD has changed significantly in recent years. Currently, the management of type II SOD remains controversial and there is a lack of non-invasive therapy options, particularly for patients not responding to endoscopic treatment. In this mini review, we aimed to discuss the current knowledge on the treatment of biliary SOD.
Interpretable machine learning model for early prediction of delirium in elderly patients following intensive care unit admission: a derivation and validation study
Authors: Tang Dayu , Ma Chengyong , Xu Yu
Published: May 2024

Background and objective

Delirium is the most common neuropsychological complication among older adults admitted to the intensive care unit (ICU) and is often associated with a poor prognosis. This study aimed to construct and validate an interpretable machine learning (ML) for early delirium prediction in older ICU patients.

Methods

This was a retrospective observational cohort study and patient data were extracted from the Medical Information Mart for Intensive Care-IV database. Feature variables associated with delirium, including predisposing factors, disease-related factors, and iatrogenic and environmental factors, were selected using least absolute shrinkage and selection operator regression, and prediction models were built using logistic regression, decision trees, support vector machines, extreme gradient boosting (XGBoost), k-nearest neighbors and naive Bayes methods. Multiple metrics were used for evaluation of performance of the models, including the area under the receiver operating characteristic curve (AUC), accuracy, sensitivity, specificity, recall, F1 score, calibration plot, and decision curve analysis. SHapley Additive exPlanations (SHAP) were used to improve the interpretability of the final model.

Results

Nine thousand seven hundred forty-eight adults aged 65 years or older were included for analysis. Twenty-six features were selected to construct ML prediction models. Among the models compared, the XGBoost model demonstrated the best performance including the highest AUC (0.836), accuracy (0.765), sensitivity (0.713), recall (0.713), and F1 score (0.725) in the training set. It also exhibited excellent discrimination with AUC of 0.810, good calibration, and had the highest net benefit in the validation cohort. The SHAP summary analysis showed that Glasgow Coma Scale, mechanical ventilation, and sedation were the top three risk features for outcome prediction. The SHAP dependency plot and SHAP force analysis interpreted the model at both the factor level and individual level, respectively.

Conclusion

ML is a reliable tool for predicting the risk of critical delirium in elderly patients. By combining XGBoost and SHAP, it can provide clear explanations for personalized risk prediction and more intuitive understanding of the effect of key features in the model. The establishment of such a model would facilitate the early risk assessment and prompt intervention for delirium.
Activity-dependent dendrite patterning in the postnatal barrel cortex
Authors: Nakagawa Naoki , Iwasato Takuji
Published: May 2024

For neural circuit construction in the brain, coarse neuronal connections are assembled prenatally following genetic programs, being reorganized postnatally by activity-dependent mechanisms to implement area-specific computational functions. Activity-dependent dendrite patterning is a critical component of neural circuit reorganization, whereby individual neurons rearrange and optimize their presynaptic partners. In the rodent primary somatosensory cortex (barrel cortex), driven by thalamocortical inputs, layer 4 (L4) excitatory neurons extensively remodel their basal dendrites at neonatal stages to ensure specific responses of barrels to the corresponding individual whiskers. This feature of barrel cortex L4 neurons makes them an excellent model, significantly contributing to unveiling the activity-dependent nature of dendrite patterning and circuit reorganization. In this review, we summarize recent advances in our understanding of the activity-dependent mechanisms underlying dendrite patterning. Our focus lays on the mechanisms revealed by in vivo time-lapse imaging, and the role of activity-dependent Golgi apparatus polarity regulation in dendrite patterning. We also discuss the type of neuronal activity that could contribute to dendrite patterning and hence connectivity.
Dolutegravir induces FOLR1 expression during brain organoid development
Authors: Caiaffa Carlo Donato , Tukeman Gabriel , Delgado Christian Zevallos , Ambekar Yogeshwari S. , Mekonnen Taye T. , Singh Manmohan , Rodriguez Victoria , Ricco Emily , Kraushaar Daniel , Aglyamov Salavat R. , Scarcelli Giuliano , Larin Kirill V. , Finnell Richard H. , Cabrera Robert M.
Published: May 2024

During the first month of pregnancy, the brain and spinal cord are formed through a process called neurulation. However, this process can be altered by low serum levels of folic acid, environmental factors, or genetic predispositions. In 2018, a surveillance study in Botswana, a country with a high incidence of human immunodeficiency virus (HIV) and lacking mandatory food folate fortification programs, found that newborns whose mothers were taking dolutegravir (DTG) during the first trimester of pregnancy had an increased risk of neural tube defects (NTDs). As a result, the World Health Organization and the U.S. Food and Drug Administration have issued guidelines emphasizing the potential risks associated with the use of DTG-based antiretroviral therapies during pregnancy. To elucidate the potential mechanisms underlying the DTG-induced NTDs, we sought to assess the potential neurotoxicity of DTG in stem cell-derived brain organoids. The gene expression of brain organoids developed in the presence of DTG was analyzed by RNA sequencing, Optical Coherence Tomography (OCT), Optical Coherence Elastography (OCE), and Brillouin microscopy. The sequencing data shows that DTG induces the expression of the folate receptor (FOLR1) and modifies the expression of genes required for neurogenesis. The Brillouin frequency shift observed at the surface of DTG-exposed brain organoids indicates an increase in superficial tissue stiffness. In contrast, reverberant OCE measurements indicate decreased organoid volumes and internal stiffness.
Primary mediastinal seminoma with azoospermia: case report and review of the literature
Authors: Li Zhiwei , Zhu Qiqi , Niu Shaorui , Xiao Kaibing , Xiao Zhiyang , Yang Pang
Published: May 2024

Introduction

Since the first report, primary mediastinal seminoma has a low incidence in the population, and it mainly affects young and middle-aged men, is clinically rare, and accounts for a very small proportion of mediastinal tumors. In this study, we describe the first case of primary mediastinal seminoma with azoospermia and hypothesize that the coexistence of the two disorders may not be a coincidence.

Case report

A 16-year-old man presented with chest tightness and chest pain, a mediastinal mass on chest CT, and abnormal 18F-fluoro-deoxyglucose uptake on a PET-CT scan. By biopsy of the mass, the pathological diagnosis was a primary mediastinal seminoma. Because chemotherapy is included in the treatment of the tumor, the patient underwent sperm freezing before treatment, considering that chemotherapy can affect fertility, but the patient was diagnosed with azoospermia. Finally, the patient underwent tumor resection and postoperative chemotherapy. No tumor recurrence was observed at the current follow-up.

Conclusion

Primary mediastinal seminoma is mainly confirmed by histopathological examination, and surgery and chemoradiotherapy are the current treatments. In patients with mediastinal seminoma or azoospermia, doctors should be aware that the two disorders may coexist, especially in men who have fertility requirements or long-term infertility, and that examination of the mediastinum and semen may lead to unexpected findings in the diagnosis and treatment. For mediastinal germ cell tumors, genetic testing is of great value in the treatment of tumors and the prediction of associated diseases. Future studies exploring the potential correlation between mediastinal seminoma and azoospermia will be prospective.
Exploring the neural basis of non-invasive prehabilitation in brain tumour patients: An fMRI-based case report of language network plasticity
Authors: Boccuni Leonardo , Roca-Ventura Alba , Buloz-Osorio Edgar , Leno-Colorado David , Martín-Fernández Jesús , Cabello-Toscano María , Perellón-Alfonso Ruben , Pariente Zorrilla Jose Carlos , Laredo Carlos , Garrido Cesar , Muñoz-Moreno Emma , Bargalló Nuria , Villalba Gloria , Martínez-Ricarte Francisco , Trompetto Carlo , Marinelli Lucio , Sacchet Matthew D. , Bartrés-Faz David , Abellaneda-Pérez Kilian , Pascual-Leone Alvaro , Tormos Muñoz Josep María
Published: May 2024

Primary brain neoplasms are associated with elevated mortality and morbidity rates. Brain tumour surgery aims to achieve maximal tumour resection while minimizing damage to healthy brain tissue. Research on Neuromodulation Induced Cortical Prehabilitation (NICP) has highlighted the potential, before neurosurgery, of establishing new brain connections and transfer functional activity from one area of the brain to another. Nonetheless, the neural mechanisms underlying these processes, particularly in the context of space-occupying lesions, remain unclear. A patient with a left frontotemporoinsular tumour underwent a prehabilitation protocol providing 20 sessions of inhibitory non-invasive neuromodulation (rTMS and multichannel tDCS) over a language network coupled with intensive task training. Prehabilitation resulted in an increment of the distance between the tumour and the language network. Furthermore, enhanced functional connectivity within the language circuit was observed. The present innovative case-study exposed that inhibition of the functional network area surrounding the space-occupying lesion promotes a plastic change in the network’s spatial organization, presumably through the establishment of novel functional pathways away from the lesion’s site. While these outcomes are promising, prudence dictates the need for larger studies to confirm and generalize these findings.
Utility of artificial intelligence in the diagnosis and management of keratoconus: a systematic review
Authors: Goodman Deniz , Zhu Angela Y.
Published: May 2024

Introduction

The application of artificial intelligence (AI) systems in ophthalmology is rapidly expanding. Early detection and management of keratoconus is important for preventing disease progression and the need for corneal transplant. We review studies regarding the utility of AI in the diagnosis and management of keratoconus and other corneal ectasias.

Methods

We conducted a systematic search for relevant original, English-language research studies in the PubMed, Web of Science, Embase, and Cochrane databases from inception to October 31, 2023, using a combination of the following keywords: artificial intelligence, deep learning, machine learning, keratoconus, and corneal ectasia. Case reports, literature reviews, conference proceedings, and editorials were excluded. We extracted the following data from each eligible study: type of AI, input used for training, output, ground truth or reference, dataset size, availability of algorithm/model, availability of dataset, and major study findings.

Results

Ninety-three original research studies were included in this review, with the date of publication ranging from 1994 to 2023. The majority of studies were regarding the use of AI in detecting keratoconus or subclinical keratoconus (n=61). Among studies regarding keratoconus diagnosis, the most common inputs were corneal topography, Scheimpflug-based corneal tomography, and anterior segment-optical coherence tomography. This review also summarized 16 original research studies regarding AI-based assessment of severity and clinical features, 7 studies regarding the prediction of disease progression, and 6 studies regarding the characterization of treatment response. There were only three studies regarding the use of AI in identifying susceptibility genes involved in the etiology and pathogenesis of keratoconus.

Discussion

Algorithms trained on Scheimpflug-based tomography seem promising tools for the early diagnosis of keratoconus that can be particularly applied in low-resource communities. Future studies could investigate the application of AI models trained on multimodal patient information for staging keratoconus severity and tracking disease progression.
A clinical evaluation and acceptability study of the innovative SurePulse VS wireless heart rate monitor across the neonatal journey
Authors: Peterson Jennifer , Jennings Clare , Mahaveer Ajit
Published: May 2024

Background

The SurePulse vital signs (VS) device is an innovative wireless heart rate monitor designed for neonatal patients. This study evaluates the application of SurePulse VS technology in clinical practice.

Methods

Data were collected about the quantitative metrics of the device itself when deployed on real infants and qualitative feedback from perinatal professionals and parents regarding their experiences using this novel technology.

Results

This study recruited 101 infants and achieved target completion rates of 101 healthcare professional (HCP) and 51 parent questionnaires over the seven-month study period. The SurePulse device was deployed across a range of gestational ages (34–39 weeks) and birth weights (1.8–3.5 kg). Device deployment was performed across a range of clinical environments, with 51% of deployments at delivery and 47% within the neonatal unit. The data show clinically acceptable timings from device deployment to heart rate signal acquisition [median 20 s (IQR 15–76 s)]. HCP feedback rated SurePulse monitoring as “Always” or “Mostly” reliable in 80% of cases. Parental feedback reported that having the SurePulse device was reassuring, convenient and beneficial to them. These positive comments were reflected across device deployment in the delivery room and within the neonatal unit.

Conclusions

The study findings show that the SurePulse device has potential to be a significant advancement in the way neonatal patients are monitored in a variety of post-delivery circumstances. This study has demonstrated that the SurePulse device has utility throughout the neonatal journey, enabling accurate heart rate monitoring in a manner that promotes parent-infant contact and bonding.
Case Report: MYH9-related disease caused by Ala44Pro mutation in a child with a previous diagnosis of chronic immune thrombocytopenia
Authors: Niwa Kaori , Toyoda Hidemi , Kohso Atsushi , Okumura Yosuke , Kunishima Shinji , Hirayama Masahiro
Published: May 2024

MYH9-related disease, a rare autosomal dominant platelet disorder characterized by thrombocytopenia, giant platelets, and leukocyte inclusion bodies, may mimic immune thrombocytopenia in children unless suspected and carefully excluded. Here, we present a case involving a three-year-old girl with mild bleeding symptoms since infancy, previously diagnosed with chronic immune thrombocytopenia. The patient exhibited isolated thrombocytopenia and lacked any family history of thrombocytopenia, hearing impairment, or renal failure. Examination of peripheral blood smears via light microscopy revealed significant platelet macrocytosis with giant platelets and basophilic Döhle-like bodies in the neutrophils. Subsequent sequencing analysis of MYH9 gene identified a p.Ala44Pro mutation. Throughout a six-year follow-up period, the patient's condition remained stable. Our report underscores the significance of identifying leukocyte inclusion bodies in peripheral blood smears and considering MYH9-related diseases, even in instances of chronic macrothrombocytopenia devoid of familial history or non-hematological manifestations.