bluuebunny/crossref_metadata_2025 huggingface dataset:
The antioxidant activity of ethanolic and aqueous extracts of dandelion (Taraxacum officinale L.)
Authors: Nowak Anna , Duchnik Wiktoria , Zielonka-Brzezicka Joanna , Muzykiewicz Anna , Florkowska Katarzyna , Klimowicz Adam , Kucharski Łukasz , Wysocka Daria , Dziedzic Agnieszka
Published: January 2020

Introduction: Dandelion (Taraxacum officinale L.) has been commonly used in traditional and contemporary medicine due to its diuretic and hepatoprotective properties. It contains polyphenols, vitamins and terpenes – i.e. compounds with antioxidant potential. Natural antioxidants protect organisms against oxidative stress, an important factor in the ageing process and in the pathogenesis of neoplastic, cardiovascular, neurodegenerative and some other diseases.

Materials and methods: Raw dandelion plant material consisted of fresh and dried leaves, flowers and roots, harvested from a natural site. The extracts for analysis were prepared using an ultrasonic bath (extraction time 15, 30 and 60 min) in water, and 40% (v/v), 70% (v/v) and 96% (v/v) ethanol mixtures used as solvents. Antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing anti-oxidant power (FRAP) methods.

Results: The antioxidant activity of dandelion depended on the type of raw material used, as well as the type of solvent and extraction time. The highest DPPH activity was found for dried flower extracts prepared in 70% ethanol for 30 min. With FRAP method, the highest reduction capacity was observed for dried leaf extracts in 40% ethanol for 30 min.

Conclusions: The antioxidant potential of ethanolic and aqueous dandelion extracts was observed. This finding suggests the usefulness of this plant as a source of antioxidants to be used in pharmaceutical and cosmetic industries.
The Ability of Different Imputation Methods for Missing Values in Mental Measurement Questionnaires
Authors: Xu Xueying , Xia Leizhen , Zhang Qimeng , Wu Shaoning , Wu Mingcheng , Liu Hongbo
Published: January 2020

Background Incomplete data are of particular important influence in mental measurement questionnaires. Most experts, however, mostly focus on clinical trials and cohort studies and generally pay less attention to this deficiency. We aim is to compare the accuracy of four common methods for handling items missing from different psychology questionnaires according to the items non-response rates. Method All data were drawn from the previous studies including the self-acceptance scale (SAQ), the activities of daily living scale (ADL) and self-esteem scale (RSES). SAQ and ADL dataset, simulation group, were used to compare and assess the ability of four imputation methods which are direct deletion, mode imputation, Hot-deck (HD) imputation and multiple imputation (MI) by absolute deviation, the root mean square error and average relative error in missing proportions of 5%, 10%, 15% and 20%. RSES dataset, validation group, was used to test the application of imputation methods. All analyses were finished by SAS 9.4. Results The biases obtained by MI are the smallest under various missing proportions. HD imputation approach performed the lowest absolute deviation of standard deviation values. But they got the similar results and the performances of them are obviously better than direct deletion and mode imputation. In a real world situation, the respondents' average score in complete data set was 28.22 ± 4.63, which are not much different from imputed datasets. The direction of the influence of the five factors on self-esteem was consistent, although there were some differences in the size and range of OR values in logistic regression model. Conclusion MI shows the best performance while it demands slightly more data analytic capacity and skills of programming. And HD could be considered to impute missing values in psychological investigation when MI cannot be performed due to limited circumstances.
Patients’ experiences of discontentment one year after total knee arthroplasty- A qualitative study
Authors: Mahdi Aamir , Svantesson Mia , Wretenberg Per , Hälleberg Maria
Published: January 2020

Background Total knee arthroplasty is a common procedure with generally good results. However, there are still patients who are discontented without known explanation. Contentment and discontentment have previously been captured by quantitative designs, but there is a lack of qualitative studies regarding these patients’ experiences. Qualitative knowledge might be useful in creating strategies to decrease the discontentment rate. Methods Of the 348 patients who responded to a letter asking if they were contented or discontented with their surgery, 61 (18%) reported discontentment. After excluding patients with documented complications and those who declined to participate, semi-structured interviews were conducted with 44 patients. The interviews were analyzed according to qualitative content analysis. The purpose was to describe patients’ experiences of discontentment one year after total knee arthroplasty. Results The patients experienced unfulfilled expectations and needs regarding unresolved and new problems, limited independence, and need for buttresses. They were bothered by pain and stiffness, and worried that changes were complications as a result of surgery. They described inability to perform daily activities and valued activities. They also felt a need for buttresses, and a lack of respect and continuity, support from health care, and information adapted to their needs. Conclusion Patient expectation seems to be the major contributing factor in patient discontentment after knee replacement surgery. This qualitative study sheds light on the meaning of unfulfilled expectations, in contrast to previous quantitative studies. The elements of unfulfilled expectations need to be dealt with both on the individual staff level and on the organizational level. For instance, increased continuity of healthcare staff and facilities may help to improve patient contentment after surgery.
Determining the feasibility and effectiveness of brief online mindfulness training for rural medical students: a pilot study
Authors: Moore Sarah Jane , Barbour Rita , Ngo Hanh , Sinclair Craig , Chambers Richard , Auret Kirsten , Hassed Craig , Playford Denese
Published: January 2020

Background We sought to determine the feasibility and effectiveness of a mindfulness training program, delivered online to medical students at a Rural Clinical School.Methods An 8-week online training program was delivered to penultimate-year medical students at an Australian Rural Clinical School during 2016. Using a mixed methods approach, we measured the frequency and duration of participants’ mindfulness meditation practice, and assessed changes in their perceived stress, self-compassion and compassion levels, as well as personal and professional attitudes and behaviours.Results 47 participants were recruited to the study. 50% of participants were practising mindfulness meditation at least weekly by the end of the 8-week program, and 32% reported practising at least weekly 4 months following completion of the intervention. There was a statistically significant reduction in participants’ perceived stress levels and a significant increase in self-compassion at 4-month follow-up. Participants reported insights about the personal and professional impact of mindfulness meditation training as well as barriers to practice.Conclusions The results provide preliminary evidence that online training in mindfulness meditation can be associated with reduced stress and increased self-compassion in rural medical students. More rigorous research is required to establish concrete measures of feasibility of a mindfulness meditation program.
Increased plasma concentration of cell-free DNA precedes disease recurrence in children with high-risk neuroblastoma
Authors: Su Yan , Wang Lijun , Jiang Chiyi , Yue Zhixia , Fan Hongjun , Hong Huimin , Duan Chao , Jin Mei , Zhang Dawei , Qiu Lihua , Cheng Xianfeng , Xu Zhong , Ma Xiaoli
Published: January 2020

Background Neuroblastoma is the third-most common cancer in children. The high rate of tumor recurrence accounts for a low survival rate in high risk neuroblastoma. Therefore it is clinically of extreme importance to find an effective biomarker for alerting disease recurrence.Methods Total 116 high risk neuroblastoma patients were recruited in Beijing Children's Hospital from February, 2015 to December, 2017. All patients had received multiple-disciplinary treatment, then went into maintenance treatment phase after evaluation. Blood samples were collected to quantify plasma cell-free DNA (cfDNA) at time points of the beginning of maintenance treatment, every three months afterwards, and diagnosis of recurrence.Results Results showed that 36 high risk neuroblastoma patients developed recurrence during maintenance treatment. The plasma cfDNA concentration was significantly higher in recurrence than in event-free patients (29.34 ng/ml VS 10.32 ng/ml). The time span of cfDNA level higher than 29 ng/ml was consistently detected ahead of recurrence at mean of 0.55 months. The ROC analysis showed that AUC was 0.825, optimal sensitivity and specificity of 80.6% and 71.3% respectively, at cfDNA level of 12.93 ng/ml.Conclusions We concluded that high level of plasma cfDNA could serve as a promising molecular marker to alert recurrence disease in high risk neuroblastoma children.
Brain SegNet: 3D Local Refinement Network for Brain Lesion Segmentation
Authors: Hu Xiaojun , Luo Weijian , Hu Jiliang , Guo Sheng , Huang Weilin , Scott Matthew R. , Wiest Roland , Dahlweid Michael , Reyes Mauricio
Published: January 2020

MR images (MRIs) accurate segmentation of brain lesions is important for improving cancer diagnosis, surgical planning, and prediction of outcome. However, manual and accurate segmentation of brain lesions from 3D MRIs is highly
expensive, time-consuming, and prone to user biases. We present an efficient yet conceptually simple brain segmentation network (referred as Brain SegNet), which is a 3D residual framework for automatic voxel-wise segmentation of brain lesion. Our model is able to directly predict dense voxel segmentation of brain tumor or ischemic stroke regions in 3D brain MRIs. The proposed 3D segmentation network can run at about 0.5s per MRIs - about 50 times faster than previous approaches [1,2]. Our model is evaluated on the BRATS 2015 benchmark for brain tumor segmentation, where it obtains state-of-the-art results, by surpassing recently published results reported in [1,2]. We further applied the proposed Brain SegNet for ischemic stroke lesion outcome prediction, with impressive results achieved on the Ischemic Stroke Lesion Segmentation (ISLES) 2017 database.
A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post–vaccination monitoring
Authors: Wang Lihua , Mi Shijiang , Madera Rachel , Ganges Llilianne , Borca Manuel V. , Ren Jingqiang , Cunningham Chase , Cino-Ozuna Ada G. , Li Hongwei , Tu Changchun , Gong Wenjie , Shi Jishu
Published: January 2020

Background: Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation of CSF vaccines. However, VNT is a time consuming procedure that requires cell culture and live virus manipulation. C-strain CSF vaccine is the most frequently used vaccine for CSF control and prevention. In this study, we presented a neutralizing monoclonal antibody (mAb) based competitive enzyme-linked immunosorbent assay (cELISA) with the emphasis on the replacement of VNT for C-strain post–vaccination monitoring.

Results: One monoclonal antibody (6B211) which has potent neutralizing activity against C-strain was generated. A novel cELISA was established and optimized based on the strategy that 6B211 can compete with C-strain induced neutralizing antibodies in pig serum to bind capture antigen C-strain E2. By testing C-strain VNT negative pig sera (n=445) and C-strain VNT positive pig sera (n=70), the 6B211 based cELSIA showed 100% sensitivity (95% confidence interval: 94.87 to 100%) and 100% specificity (95% confidence interval: 100 to 100%). The C-strain antibody can be detected in pigs as early as 7 days post vaccination with the cELISA. By testing pig sera (n=139) in parallel, the cELISA showed excellent agreement (Kappa=0.957) with VNT. The inhibition rate of serum samples in the cELISA is highly correlated with their titers in VNT (r 2 =0.903, p<0.001). In addition, intra- and inter-assays of the cELISA exhibited acceptable repeatability with low coefficient of variations (CVs).

Conclusions: This novel cELISA demonstrated excellent agreement and high level correlation with VNT. It is a reliable tool for sero-monitoring of C-strain vaccination campaign because it is a rapid, simple, safe and cost effective assay that can be used to monitor vaccination-induced immune response at the population level.
Association of IL-10 gene promoter polymorphisms with susceptibility to pseudoexfoliation syndrome, pseudoexfoliative and primary open-angle glaucoma
Authors: Fakhraie Ghasem , Parvini Farshid , Ghanavi Jalaledin , Saif Shima , farnia poopak
Published: January 2020

Background: The involvement of cytokines in pathogenesis of pseudoexfoliation syndrome and glaucoma has been demonstrated in several studies. The aim of the present study was to explore the association between three promoter polymorphisms -592C/A (rs1800872), -819C/T (rs1800871) and -1082A/G (rs1800896) of interleukin 10 (IL-10) gene with susceptibility to pseudoexfoliation syndrome (PEX), pseudoexfoliative glaucoma (PEXG), and primary open-angle glaucoma (POAG). Methods: In this study, 114 PEX, 118 PEXG, 114 POAG patients and 126 healthy individuals from Iranian population were participated. Detailed ophthalmic examinations by an ophthalmologist including slit-lamp bio-microscopic examination, dilated examination of the lens, gonioscopy, and funduscopy were carried out on patients and controls. Genomic DNA was extracted from the blood samples and ARMS–PCR was performed to detect promoter polymorphisms of IL-10. Results: In all three SNPs studied, there was a significant difference in the genotype distribution between patients and control subjects. Results revealed that the AA genotype of IL-10 -592C/A SNP is associated with PEX. However, TT genotype of -819C/T and AA genotype of -1082A/G SNP are significantly associated with susceptibility to either PEX or PEXG and POAG disorders. Furthermore, the ACC haplotype containing the IL-10 -1082A allele was associated with PEX ( P = 0.02, OR = 5.76, 95 % CI = 5.17-24.49), PEXG ( P = 0.006, OR = 7.54, 95 % CI = 6.62-30.76) and POAG ( P = 0.003, OR = 8.11, 95 % CI = 7.13-33.15). Conclusions: Our results demonstrated that IL-10 gene promoter polymorphisms are associated with susceptibility to PEX, PEXG and POAG in Iranian population. Considering the fact that IL-10 polymorphisms are associated with various IL-10 expressions, further research is needed to explain its involvement in these disorders and the formation of extracellular fibrillar amyloid deposits in PEX and PEXG.
Nomogram Model for Predicting Cause-Specific Mortality in Patients with Stage I Small-Cell Lung Cancer: A Competing Risk Analysis
Authors: Li Jianjie , Zheng Qiwen , Zhao Xinghui , Zhao Jun , An Tongtong , Wu Meina , Wang Yuyan , Zhuo Minglei , Zhong Jia , Yang Xue , Jia Bo , Chen Hanxiao , Dong Zhi , Wang Jingjing , Zhai Xiaoyu , Chi Yujia , wang ziping
Published: January 2020

Background: The five-year cumulative incidence rate in patients diagnosed with stage I small-cell lung cancer (SCLC) who were instructed to undergo surgery was from 40% to 60%.The death competition influence the accuracy of the classical survival analyses.The aim of the study is to investigate the mortality of stage I small-cell lung cancer (SCLC) patients in the presence of competing risks according to a proportional hazards model, and to establish a competing risk nomogram to predict probabilities of both cause-specific death and death resulting from other causes.
Methods: The study subjects were patients diagnosed with stage I SCLC according to ICD-O-3.First,the cumulative incidence functions (CIFs) of cause-specific death, as well as of death resulting from other causes, were calculated. Then, a proportional hazards model for the sub-distribution of competing risks and a monogram were constructed to evaluate the probability of mortality in stage I SCLC patients.
Results: 1811 patients were included in this study. The five-year probabilities of death due to specific causes and other causes were 61.5% and 13.6%, respectively. Tumor size, extent of tumor, surgery, and radiotherapy were identified as the predictors of death resulting from specific causes in stage I SCLC. The results showed that surgery could effectively reduce the cancer-specific death, and the one-year cumulative incidence dropped from 34.5% to 11.2%.Like surgery, chemotherapy and radiotherapy improved the one-year survival rate.
Conclusions: We constructed a predictive model for stage I SCLC using the data from the SEER database. The proportional sub-distribution models of competing risks revealed the predictors of death resulting from both specific causes and other causes. The competing risk nomogram that we built to predict the prognosis showed good reliability and could provide beneficial and individualized predictive information for stage I SCLC patients.
Compliance with spectacle use in children with refractive errors- a systematic review and meta-analysis
Authors: Dhirar Nonita , Dudeja Sankalp , Duggal Mona , Gupta Parul Chawla , Jaiswal Nishant , Singh Meenu , Ram Jagat
Published: January 2020

Background Primary objective of this review was to measure compliance with spectacle use in children with refractive errors. Secondary objective was to understand the reasons for non-compliance.Methods The databases searched were Ovid, EMBASE, CINAHL and Pubmed. All studies up to March, 2018 were included. The search terms were- ((((((Compliance [Title/Abstract]) OR Adherence[Title/Abstract]) OR Compliant[Title/Abstract]) OR Adherent[Title/Abstract])) AND (((Spectacle[Title/Abstract]) OR Spectacles[Title/Abstract]) OR Eye Glasses[Title/Abstract])) AND ((((Child[Title/Abstract]) OR Children[Title/Abstract]) OR Adolescent[Title/Abstract]) OR Adolescents[Title/Abstract]). Two researchers independently searched the databases and initial screening obtained 33 articles. The PRISMA guidelines were followed for conducting and writing the systematic review. Two reviewers assessed data quality independently using the Quality Assessment tool for systematic reviews of observational studies (QATSO). Poor quality studies were those, which had a score of less than 33% on the QATSO tool. Sensitivity analysis was done to determine if poor quality studies effected compliance. Galbraith plot was used to investigate statistical heterogeneity amongst studies. A random effects model was used to pool compliance.Results Twenty-three studies were included in the review, of which 20 were included in the quantitative analysis. All the studies were cross sectional. The overall compliance with spectacle use was 40.14% (95% CI- 32.78-47.50). The compliance varied from 9.84% (95% CI=2.36-17.31) to 78.57% (95% CI=68.96-88.18). The compliance derived in sensitivity analysis was 40.09%. Reasons for non-compliance were broken/lost spectacles, forgetfulness, and parental disapproval.Conclusion Appropriate remedial measures such as health education and strengthening vision care services will be required to address poor compliance with spectacle use among children.
Short-term Outcomes of Robotic- versus Laparoscopic-Assisted Total Gastrectomy for Advanced Gastric Cancer: A Propensity Score Matching Study
Authors: Yang Changdong , Shi Yan , Xie Shaohui , Chen Jun , Zhao Yongliang , Qian Feng , Hao Yingxue , Tang Bo , Yu Pei-Wu
Published: January 2020

Background: Few studies have been designed to evaluate the short-term outcomes for advanced gastric cancer (AGC) between robotic-assisted total gastrectomy (RATG) and laparoscopy-assisted total gastrectomy (LATG) alone. The purpose of this study was to assess short-term outcomes of RATG compared with LATG for AGC.
Methods: We retrospectively evaluated 116 and 244 patients who underwent RATG or LATG respectively. Besides, we performed a propensity score matching (PSM) analysis between RATG and LATG for clinicopathological characteristics to reduce bias and compared short-term surgical outcomes.
Results: After PSM, the RATG group had longer operation time (291.09±58.41 vs. 271.99±48.41min, p=0.007), less intraoperative bleeding (151.98±92.83 vs. 172.59±97.01ml, p=0.032) and more N2 tier retrieved lymph nodes (RLNs) (9.33±5.46 vs. 7.50±3.86, p=0.018) than the LATG group. Besides, the total RLNs of RATG was more but not statistically significant compared to LATG (35.09±12.93 vs.32.34±12.05, p=0.062). However, no significant differences were found between the two groups in terms of length of incision, proximal resection margin, distal resection margin, postoperative hospital stay. The conversion rate was 4.92% and 8.61% in the RATG and LATG groups, respectively, with no significant difference (p=0.198). The ratio of splenectomy was 1.7% and 0.4% respectively (p=0.503). There was no significant difference in overall complication rate between RATG and LATG groups after PSM (24.1% vs. 33.6%; p=0.102)and the grade II complications accounted for most of all complications in the two cohorts. The mortality was 0.9% and 0% respectively (p=0.322).
Conclusion : This study demonstrates that RATG is comparable to LATG in terms of short-term surgical outcomes.
Evaluation of radioactive 125I seed implantation for the treatment of refractory malignant tumors based on CT-guided 3D template assisted technique: efficacy and safety
Authors: Zhao Guang Sheng , Liu Song , Yang Liang , Li Chuang , Wang Ruo Yu , Zhou Jun , Zhang Yue Wei
Published: January 2020

Background: To observe the medium-and long-term clinical efficacy and safety of radioactive 125I seed implantation for refractory malignant tumors based on a CT-guided 3D template-assisted technique.
Methods: Twenty-five patients with refractory malignant tumors who received radioactive 125I seed implantation based on a CT-guided 3D template-assisted technique were selected. The post-operative adverse reactions were recorded; the number of puncture needles and particles used in the operation, dosimetric parameters, post-operative physical strength scores, and tumor response were statistically analyzed. The overall survival time and survival rate were counted, and the effect and prognosis were assessed.
Results: 125I seed implantation was successful in all patients and no serious complications occurred. The average number of implanted puncture needles was 17 (19.12±13.00) and the median number of particles was 52 (55.12±32.97). The D90 in the post-operative clinical target volume (CTV) (93.24±15.70 Gy) was slightly lower than pre-operative CTV (93.92±17.60 Gy; P>0.05). The D90 in the postoperative planning target volume (PTV) (142.16±22.25 Gy) was lower than the pre-operative PTV (145.32±23.48 Gy; P>0.05). The tumor responses at 6 months post-operatively: complete remission (CR), 20%(5/25); partial remission (PR), 48%(12/25); stable disease (SD), 24%(6/25); progressive disease (PD), 8%(2/25); CR+PR, 68%(17/25); and local control rate, 92%(23/25). The 6-, 12-, and 24-month survival rates were 100%, 88%, and 52%, respectively. The post-operative physical strength score (Karnofsky performance score, KPS) exhibited a gradual trend towards recovery, which rose to the highest value 12 months after seed implantation, then decreased slightly, but the average score was still > 90 points. There was one intra-operative pneumothorax and two patients with superficial malignant tumors developed skin ulcerations. Multivariate analysis of prognosis showed that tumor sites and types were independent risk factors affecting survival. The number of needles and particles, and template type were not factors that affected prognosis.
Conclusions: 3D template combined with CT-guided radioactive 125I seed implantation was shown to improve the rational distribution of radiation dose in the tumor target area because accurate radioactive 125I particle implantation was achieved. It has fewer complications and can further extend the overall survival and quality of life in patients with refractory malignant tumors.
Identification and Analysis of the FAD Gene Family in Walnuts (Juglans regia L.) Based on Transcriptome Data
Authors: Liu Kai , Zhao Shugang , Wang Shuang , Wang Hongxia , Zhang Zhihua
Published: January 2020

Background: Walnut fatty acids, the main component of walnut kernels, contain a large amount of unsaturated fatty acids, such as linoleic acid and linolenic acid, which are essential fatty acids in humans and have important effects on human growth and health. Fatty acid desaturase (FAD) is widely distributed throughout the biological world. Its main function is to remove hydrogen from carbon chains in the biosynthesis of unsaturated fatty acids to synthesize C=C double bonds.

Results: In the current research, 24 members of the JrFAD gene family were identified by bioinformatics analysis; the expression of fatty acid synthesis genes in walnut kernels at different developmental stages was analysed by transcriptome sequencing, and the expression of JrFAD3-1 , an enzyme gene for linolenic acid synthesis, was particularly prominent. The results showed that the relative expression level of FAD3-1 changed dramatically with the kernel development stage, and the expression changes showed a "bell shape". There was a significant positive correlation between the expression of JrFAD3-1 from 90-100 DAF (days after flower) and the content of alpha-linolenic acid from 100-130 DAF, with a correlation coefficient of 0.991. JrFAD3-1 can be considered closely related to Betula pendula and Corylus heterophylla .

Conclusion: 24 members of the JrFAD gene family consist of four subfamilies: the SAD desaturase subfamily, Δ7/Δ9 desaturase subfamily, Δ12/ω-3 desaturase subfamily and "front-end" desaturase subfamily. A key gene Jr FAD3-1 was obtained based on transcriptome data and there was a significant positive correlation between the expression of JrFAD3-1 from 90-100 DAF and the content of alpha-linolenic acid from 100-130 DAF. JrFAD3-1 can be considered closely related to Betula pendula and Corylus heterophylla .
Does photobiomodulation therapy combined to static magnetic field (PBMT-sMF) promote ergogenic effects even when the non-exercised leg is irradiated? A randomized, triple-blind, placebo-controlled trial.
Authors: Machado Caroline dos Santos Monteiro , Casalechi Heliodora Leão , Vanin Adriane Aver , de Azevedo Jônatas Bezerra , de Carvalho Paulo de Tarso Camillo , Leal-Junior Ernesto Cesar Pinto
Published: January 2020

Background The direct application of photobiomodulation therapy (PBMT) combined with a static magnetic field (sMF) (PBMT-sMF) to target tissues is shown to improve muscle performance and recovery. Studies have reported possible PBMT induced systemic effects. Notably, the extent of these effects on musculoskeletal performance and the optimal site of application remain unclear, although this information is clinically important because these factors directly affect the magnitude of the effect. We investigated the effects of PBMT-sMF on musculoskeletal performance and post-exercise recovery of muscles in exercised and non-exercised legs before the implementation of an exercise protocol.Methods This randomized, triple-blind placebo-controlled study included 30 healthy men randomly assigned to the placebo, exercised-leg, and non-exercised leg groups. Active or placebo PBMT-sMF was applied to 6 sites of the quadriceps muscle of both legs. An eccentric exercise protocol was used to induce fatigue. The following parameters were analyzed to evaluate exercise performance: peak torque assessed by maximal voluntary contraction (MVC), delayed muscle pain assessed by the visual analogue scale (VAS), muscle injury assessed by serum creatine kinase (CK), and fatigue assessed by serum lactate levels. Evaluations were performed before implementation of the eccentric (baseline) exercise protocol, as well as immediately after and 1, 24, 48, and 72 hours upon protocol completion. The Bonferroni post-hoc ANOVA test was used considering the level of statistical significance p <0.05.Results Compared to the placebo and systemic groups, irradiation with PBMT-SMF led to statistically significant improvement (p <0.05) with regard to all variables in the exercised-leg group. Results of irradiation in the non-exercised leg group were similar to those in the placebo group with regard to all variables.Conclusion Our results support the current evidence that irradiation of all exercised muscles produces ergogenic effects. PBMT-sMF improved performance and reduced muscle fatigue only when applied locally to muscles involved in physical activity.
The effects of exergaming on pain, postural control, technology acceptance and flow experience, in older people with chronic musculoskeletal pain: a randomised controlled trial
Authors: Ditchburn Jae-Llane , Schaik Paul van , Dixon John , MacSween Alasdair , Martin Denis
Published: January 2020

Background : Previous studies of exergaming for older people have reported mixed findings, but, overall, there is a broad indication of potential benefit. The purpose of this study was to assess the effects of exergaming, comparing the Interactive Rehabilitation and Exercise System (IREX®) with traditional gym-based exercise with no virtual stimuli (TGB), on pain, postural control, technology acceptance and flow experience in older people with chronic musculoskeletal pain. Methods : 54 older adults (age: 71 ± 5 years) with chronic musculoskeletal pain were randomised (stratified, blind card) to one of two groups: (a) completed exergaming using IREX® and (b) completed TGB exercise. Both groups completed two, 40-minute, exercise sessions (matched for intensity, duration and movement patterns) a week for six weeks. The sensory, emotional and motivational dimension of pain was measured using the Multidimensional Affect and Pain Survey (MAPS) questionnaire and intensity, with a numeric rating scale. Postural Control was measured as sway using a Kistler™ force platform. Technology acceptance was measured with the Unified Theory of Acceptance and Use of Technology (UTAUT) and flow experience with the Flow State Scale (FSS) questionnaires. Rating of Perceived Exertion (BORG RPE) and HR were also recorded during all sessions. Results : There were no significant differences in objective or subjective measures of physical demand and hence the exercise groups were matched. There were significant improvements in pain and balance in the exergaming group compared to the TGB group. Although significant intervention effects on technology acceptance were found in social influence and behavioural intention in the TBG group, both groups demonstrated significant increases in all of the technology acceptance variables over time. In terms of flow experience, concentration at task was significantly influenced in the TGB group and significant increases in flow experience variables were observed in both groups (TGB and exergaming). Conclusion : Exergaming has potential to alleviate pain and improve balance in older people with chronic musculoskeletal pain. Both forms of exercise are acceptable, intrinsically motivating and show evidence of benefit to older people with chronic musculoskeletal pain.
Genetic polymorphism of the CYP2C19 gene in the Foshan area of Guangdong Province
Authors: yuan Xiao wen , Yuan Shi-yun , Qiu Quan , Luo Zi-yuan , Zhao Xue-feng
Published: January 2020

Background: The CYP2C19 gene is highly polymorphic, and CYP2C19 is involved in the broad interindividual variability of the clinical efficacy of certain clinical medications, such as clopidogrel. However, data on the CYP2C19 genotype in the Chinese population of the Foshan area of Guangdong Province are scarce. The purpose of this study was to determine CYP2C19 genetic polymorphisms in patients in the Foshan area and to compare the CYP2C19 genotype frequencies in different populations to determine the allele distribution pattern to identify the most appropriate prescription. Methods: The CYP2C19 gene was detected in 1231 patients on a gene chip platform, and the genotype frequencies of CYP2C19 in Foshan populations from different populations were compared. Results: The frequencies of CYP2C19*1, *2 and *3 in the Foshan population were 63.89%, 30.46% and 5.65%, respectively. For the three metabolic types, the frequency associated with the rapid metabolism type (*1/*1) was 41.51 [95% confidence interval (CI) 40.11 to 42.91%]; that for the intermediate metabolism type (*1/*2, *1/*3) was 44.76% (95% CI 43.34 to 46.18and that for the slow metabolism type (*2/*2, *2/*3, *3/*3) was 13.73% (95% CI 12.75 to 14.71%). In the Foshan population, the frequencies of the CYP2C19 *2 and *3 alleles were similar to those previously reported for Chinese and other Asian populations. Conclusion: Our study the genetic basis of CYP2C19 polymorphism in the Foshan population. Our results will potentially contribute to the improvement of pharmacotherapy effectiveness by providing personalized medicine for the Foshan population. Key words: Polymorphism; Genetic; Pharmacogenetics
Emerging high-risk ST101 and ST307 carbapenem-resistant Klebsiella pneumoniae clones from bloodstream infections in Southern Italy
Authors: Loconsole Daniela , Accogli Marisa , Lisa De Robertis Anna , Loredana Capozzi , Angelica Bianco , Anna Morea , Rosanna Mallamaci , Michele Quarto , Antonio Parisi , Chironna Maria
Published: January 2020

BackgroundCarbapenem-resistant Klebsiella pneumoniae (CR-KP) is an urgent public health issue in Italy. The pattern of resistance is due mainly to dissemination of carbapenemase genes. Molecular characterization of carbapenem-resistant Klebsiella pneumoniae (CR-KP) strains was performed over a three-year period. In-depth analysis was performed on a subset of emerging CR-KP ST101 and ST307 clones.MethodsA prospective study was performed on 691 patients with CR-KP bloodstream infections hospitalized in 19 hospitals located in three large provinces in Southern Italy. Carbapenemase genes were identified via genotyping methods. Multi-locus sequence typing (MLST) and Whole Genome Sequencing (WGS) were carried out on ST101 and ST307 isolates.ResultsAmong the CR-KP isolates, blaKPC was found in 95.6%, blaVIM was found in 3.5%, blaNDM was found in 0.1% and blaOXA-48 was found in 0.1%. The blaKPC-3 variant was identified in all 104 characterized KPC-KP isolates. MLST of 231 representative isolates revealed ST512 in 45.5%, ST101 in 20.3% and ST307 in 18.2% of the isolates. cgMLST of ST307 and ST101 isolates revealed presence of more than one beta-lactam resistance gene. Amino acid substitution in the chromosomal colistin-resistance gene pmrB was found in two ST101 isolates.ConclusionsST512 is widespread in Southern Italy, but ST101 and ST307 are emerging since they were found in a significant proportion of cases. Aggressive infection control measures and a continuous monitoring of these high-risk clones are necessary to avoid rapid spread of CR-KP, especially in hospital settings.
Research priorities for rehabilitation and aging with HIV: a framework from the Canada-International HIV and Rehabilitation Research Collaborative (CIHRRC)
Authors: O'Brien Kelly K , Ibáñez-Carrasco Francisco , Solomon Patricia , Harding Richard , Brown Darren , Ahluwalia Puja , Carusone Soo Chan , Baxter Larry , Emlet Charles , Restall Gayle , Casey Alan , Ahluwalia Amrita , Quigley Adria , Terpstra Alex , Iku Nkem
Published: January 2020

BackgroundPeople living with HIV are living longer, and can experience physical, mental and social health challenges associated with aging and multimorbidity. Rehabilitation is well positioned to address disability and maximize healthy aging. An international collaborative network, called the Canada-International HIV and Rehabilitation Research Collaborative (CIHRRC), works to guide this emerging field.In this article, we report findings from CIHRRC’s aim to identify emerging research priorities in HIV, aging and rehabilitation from the perspectives of people living with HIV, clinicians, researchers, representatives from community organizations and policy stakeholders.MethodsWe conducted a multi-stakeholder mixed-method international consultation with people living with HIV, researchers, clinicians and representatives of community-based organizations to identify research priorities in HIV, aging and rehabilitation. Stakeholders identified research priorities during a one-day International Forumcomprised of presentations and facilitated discussion. We collated and analyzed data using content analytical techniques, resulting in a framework of research priorities.ResultsSixty-nine stakeholders from countries including Canada (n=62;90%), the United Kingdom (n=5;7%), United States (n=1;1%) and Australia (n=1;1%) attended the International Forum on HIV, Aging and Rehabilitation. Stakeholders represented community-based organizations (n=20;29%), academic institutions (n=18;26%), community or institutional healthcare organizations (n=11;16%), research or knowledge production organizations (n=10;14%), and organizations representing government or industry (n=10;14%). TheFramework of Research Priorities in HIV, Aging and Rehabilitationincludes seven research priorities: 1) nature, extent and impact of disability, concurrent health conditions and chronic inflammation with HIV; 2) prevalence, severity and impact of frailty; 3) community and social participation aging with HIV; 4) strategies for chronic disease management and healthy aging with HIV; 5) facilitators and barriers to access to and engagement in, rehabilitation; 6) effectiveness of rehabilitation interventions for healthy aging with HIV; and 7) advancing development and use of patient reported outcome measures in HIV and aging. The Framework highlights methodological considerations to approach the priorities and the importance of knowledge translation and exchange to apply research knowledge into practice, programs and policy.ConclusionsThese priorities offer a foundation for collaboration among international and multidisciplinary teams to advance the field of HIV, aging and rehabilitation in order to promote healthy aging with HIV.
Etoricoxib decreases mouse subchondral bone mass and biomechanical properties in early osteoarthritis
Authors: Liu Bo , Ji Chen-Chen , Shao Yi-Jie , Liang Ting , He Jia-Heng , Jiang Hua-Ye , Chen Guang-Dong , Luo Zong-Ping
Published: January 2020

BackgroundEtoricoxib, a selective Cyclooxygenase-2 (COX-2) inhibitor, is commonly used in osteoarthritis (OA) for pain relief. The purpose of our study was to investigate the effects of Etoricoxib on mouse subchondral bone in early OA.MethodsOA was induced via destabilization of the medial meniscus (DMM) in C57BL/6J mice. After surgery, the mice were randomly and equally divided into five groups: a sham-operated control group (Sham group), an osteoarthritis (OA) group (DMM group), an OA treated with Etoricoxib 5mg/kg (DMM+E5) group, an OA treated with Etoricoxib 10mg/kg (DMM+E10) group, and an OA treated with Etoricoxib 20mg/kg (DMM+E20) group. Mice in the Sham group and DMM group were injected with a similar dose of vehicle (40% ethyl alcohol–saline solution). Four weeks after treatment, mice were euthanized. Micro computed tomography (Mirco-CT) analysis, Safranin O-Fast Green staining, hematoxylin and eosin (HE) staining were performed to evaluate morphological and structural changes. In addition, atomic force microscopy (AFM) analysis was performed to evaluate changes in the elastic modulus. Furthermore, changes in microstructure were detected by scanning electron microscopy (SEM).ResultsEtoricoxib inhibited osteophyte formation in the subchondral bone. However, it also reduced the bone volume fraction (BV/TV), lowered trabecular thickness (Tb.Th), and more microfractures and pores were observed in the subchondral bone. Moreover, Etoricoxib reduced the elastic modulus of subchondral bone. Furthermore, exposure to Etoricoxib further increased the empty/total osteocyte ratio of the subchondral bone. In cartilage and synovium, Etoricoxib did not significantly change the Osteoarthritis Research Society International (OARSI) score, the modified Mankin score, and the synovialitis-score versus the DMM group. Conclusion﻿Our results demonstrate that although Etoricoxib can relieve the pain induced by OA, it also has adverse effects on subchondral bone in early OA.
A Comparison of Global Surgery Tariffs and the Actual Cost of Bills at Hazrate Rasoole Akram Educational and Medical Center
Authors: Abutorabi Ali , Radinmanesh Maryam , Rezapour Aziz , Afshari Mahnaz , Taheri Ghasem
Published: January 2020

Backgroundthe health service tariff is an appropriate policymaking tool and the financial leverage of the health system control which affects quality, availability, cost, efficiency, equity and accountability of health services. Global surgeries include 91 common cases of general and specialized surgeries in hospitals; fixed tariffs are annually defined for these surgeries, and insurance companies must pay medical centers based on these tariffs. The aim of this study was to examine and compare hospital bills with global surgery tariffs at Hazrate Rasoole Akram Educational and Medical Center in 2017.MethodsThis descriptive-analytic study was conducted retrospectively and compared the global and actual costs of global surgeries performed in the third quarter of the year 2017 at Hazrate Rasoole Akram Educational and Medical Center. Required data on the actual costs of surgeries was collected through the Hospital Information System (HIS) and patients’ records. Information on the global costs was obtained from the Annual Circulars of Insurance Council for the studied period about the cost of global surgeries. Linear regression (STATA13 software) was used to investigate the effect of items on tariff and invoice differences; concerning other calculations, EXCEL software was used.ResultsThe highest frequency of global surgeries was related to ophthalmic surgery which accounted for approximately half of total surgeries performed at Hazrate Rasoole Akram Hospital. The most significant difference between global tariff and invoice was also related to ophthalmic surgery (6502876538 Rials a year).Overall, the actual hospital bills were much higher than the tariffs approved for global surgeries, and the total difference was 15913703073 Rials. The results revealed that there was a significant relationship between some of the items such as the cost of operating rooms, anesthesia and other services.ConclusionsReferral hospitals which are at the level three of referral networks usually treat more complex patients; this should be taken into account when defining surgery tariffs of these centers. On the other hand, hospitals need to control the costs and reduce the end cost of these surgeries by improving clinical management and cost management. In addition, prospective and case-based payment methods can control health costs.
Racial/Ethnic differences in Metabolic Syndrome in high-income countries: a systematic review protocol
Authors: Adjei Nicholas Kofi , Samkange-Zeeb Florence , Kebede Mihiretu , Saleem Maham , Heise Thomas L , Zeeb Hajo
Published: January 2020

BackgroundMetabolic Syndrome (MetS) is a constellation of several known cardiovascular and type 2 diabetes risk factors, including hyperglycemia, obesity, dyslipidemia and hypertension. While there is some evidence that MetS differ between some population subgroups (i.e., sex, race and ethnicity), not much is known about patterns of MetS among different racial/ethnic groups. The aim of this review is to systematically assess the literature on the prevalence of MetS between racial/ethnic groups, including migrant populations in high-income countries. MethodsThe preferred reporting items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines will be followed and the following databases searched for relevant studies: MEDLINE, the Social Science Citation Index (SSCI), the Science Citation Index (SCI), the Cumulative Index to Nursing & Allied Health Literature (CINAHL), and the Cochrane Library databases. A combination of keywords and subject headings will be used in each database to cover all aspects of racial and ethnic differences (i.e. race, ethnicity, minority populations) as well as metabolic syndrome. Cross-sectional, case-control, cohort and ecological studies on MetS that meet the inclusion criteria will be synthesised using narrative synthesis, and the results will be presented using reported outcome statistics (i.e., incidence and prevalence). ConclusionThis is the first review that we are aware of to systematically synthesize and collate the available evidence on the frequency, incidence or prevalence of MetS between racial/ethnic groups, including migrant populations. The findings may guide policy formulation and at the same time highlight gaps in the literature that future research should address.
Dog importation and changes in canine intestinal nematode prevalence in Colorado 2013-2017
Authors: Drake Jason , Parrish Rudolph
Published: January 2020

BackgroundAnimal rescue and animal welfare organizations are relocating thousands of dogs per year following natural disasters and in attempts to provide greater adoption opportunities. Many dogs are sourced from the southeastern USA, which historically has a high prevalence rate for many parasites and parasitic diseases. The Colorado Department of Agriculture Pet Animal Care Facilities Act (PACFA) requires animal shelters and animal welfare organizations to report annually a variety of statistics including the numbers of dogs imported into Colorado from out of state. The Companion Animal Parasite Council (CAPC) provides data nationally, down to the state and county level, on a variety of common parasitic and vector borne diseases. This data makes it possible to track changes in parasite prevalence over several years. MethodsTest results for canine roundworm, hookworm and whipworm were collected from the CAPC maps for 2013-2017. Dog importation data for 2014-2017 was collected from PACFA reports. For evaluation of the statistical significance of prevalence changes when comparing 2013 to 2017, 2 × 2 contingency tables were constructed with both positive and negative test results for each year and the data assessed using Chi-square tests to determine if the 2017 prevalence was significantly different than the 2013 prevalence for roundworm, hookworm and whipworm.ResultsSignificant increases in intestinal nematode prevalence occurred in Colorado from 2013 to 2017. The prevalence of canine roundworm rose 35.60%, the prevalence of canine hookworm rose 137.33% and the prevalence of canine whipworm rose 63.68%. From 2014-2017, over 114,000 dogs were transported into Colorado from out-of-state, by more than 130 animal shelters and rescue organizations. Three of the larger organizations reported that the majority of their dogs were obtained from New Mexico, Texas and Oklahoma. Texas and Oklahoma have historically much higher parasite prevalence than Colorado. ConclusionsVeterinarians in areas with historically low parasite prevalence where dogs from high parasite prevalence areas are arriving for adoption may need to reevaluate their recommendations regarding fecal examination and deworming frequencies as historic levels of intestinal parasite infection may no longer be accurate assessments of future infection risks.