bluuebunny/crossref_metadata_2025 huggingface dataset:
Phytofabrication of Silver Nanoparticles (AgNPs) with Pharmaceutical Capabilities Using Otostegia persica (Burm.) Boiss. Leaf Extract
Authors: Sharifi-Rad Majid , Pohl Pawel , Epifano Francesco
Published: April 2021

In the last years, the plant-mediated synthesis of nanoparticles has been extensively researched as an affordable and eco-friendly method. The current study confirms for the first time the capability of the Otostegia persica (Burm.) Boiss. leaf extract for the synthesis of silver nanoparticles (AgNPs). The phytofabricated AgNPs were characterized by ultraviolet–visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and zeta potential analysis. Moreover, the total phenolic and flavonoids contents, and the antioxidant, antibacterial, antifungal, and anti-inflammatory properties of the phytofabricated AgNPs and the O. persica leaf extract were assessed. The results showed that the produced AgNPs were crystalline in nature and spherical in shape with an average size of 36.5 ± 2.0 nm, and indicated a localized surface plasmon resonance (LSPR) peak at around 420 nm. The zeta potential value of −25.2 mV pointed that the AgNPs were stable. The phytofabricated AgNPs had lower total phenolic and flavonoids contents than those for the O. persica leaf extract. The abovementioned AgNPs showed a higher antioxidant activity as compared with the O. persica leaf extract. They also exhibited significant antibacterial activity against both Gram-positive (Staphylococcus aureus, Bacillus subtilis, and Streptococcus pyogenes) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) bacteria. In addition, appropriate antifungal effects with the minimum inhibitory concentration (MIC) values of 18.75, 37.5, and 75 µg mL−1 against Candida krusei, Candida glabrata, and Candida albicans, respectively, were noted for this new bionanomaterial. Finally, the phytofabricated AgNPs showed dose-dependent anti-inflammatory activity in the human red blood cell (RBC) membrane stabilization test, being higher than that for the O. persica leaf extract. The resulting phytofabricated AgNPs could be used as a promising antioxidant, antibacterial, antifungal, and anti-inflammatory agent in the treatments of many medical complications.
Gastric Cancer and the Daily Intake of the Major Dish Groups Contributing to Sodium Intake: A Case-Control Study in Korea
Authors: Kwak Jung-Hyun , Eun Chang-Soo , Han Dong-Soo , Kim Yong-Sung , Song Kyu-Sang , Choi Bo-Youl , Kim Hyun-Ja
Published: April 2021

Studies on the association between gastric cancer (GC) and the intake of soup-based dish groups (noodles and dumplings, soups, and stews), which are sodium-contributing foods, in Korea are insufficient, and the results of studies on the intake of pickled vegetables such as kimchi are inconsistent. This study aimed to determine the association between the incidence of GC and the daily intake of high-sodium dish groups (noodles and dumplings, soups, stews, and pickled vegetables) and whether these associations differ depending on behavioral risk factors for GC. In this case-control study, subjects aged 20–79 years were recruited from two hospitals between December 2002 and September 2006. A total of 440 cases and 485 controls were recruited, of which 307 pairs were matched and included for the analysis. In our results, a higher intake of noodles and dumplings was associated with a significantly increased incidence of GC. In the participants who consumed past or current alcohol, a higher intake of noodles and dumplings was associated with a significantly increased incidence of GC. Our results suggest that efforts to reduce the daily sodium intake from noodles and dumplings are needed to prevent and reduce the incidence of GC.
A Remarkable Genetic Diversity of Rotavirus A Circulating in Red Fox Population in Croatia
Authors: Čolić Daniel , Krešić Nina , Mihaljević Željko , Andreanszky Tibor , Balić Davor , Lolić Marica , Brnić Dragan
Published: April 2021

Rotaviruses (RV), especially Rotavirus A (RVA), are globally recognized as pathogens causing neonatal diarrhea, but they also affect intensive animal farming. However, the knowledge on their significance in wildlife is rather limited. The aim of the study was to unveil the prevalence, molecular epidemiology, and genetic diversity of RVA strains circulating in the red fox (Vulpes vulpes) population in Croatia. From 2018 to 2019, 370 fecal samples from fox carcasses hunted for rabies monitoring were collected. All samples were first tested using a VP2 real-time RT-PCR; in the subsequent course, positives were subjected to VP7 and VP4 genotyping. The results revealed an RVA prevalence of 14.9%, while the circulating RVA strains showed a remarkable genetic diversity in terms of 11 G and nine P genotypes, among which one G and three P were tentatively identified as novel. In total, eight genotype combinations were detected: G8P[14], G9P[3], G9P[23], G10P[11], G10P[3], G11P[13], G15P[21], and G?P[?]. The results suggest a complex background of previous interspecies transmission events, shedding new light on the potential influence of foxes in RVA epidemiology. Their role as potential reservoirs of broad range of RVA genotypes, usually considered typical solely of domestic animals and humans, cannot be dismissed.
Rapid Detection of blaKPC-9 Allele from Clinical Isolates
Authors: Gartzonika Konstantina , Bozidis Petros , Priavali Ephthalia , Sakkas Hercules
Published: April 2021

The emergence of Klebsiella pneumoniae carbapenemase (KPC) nosocomial outbreaks related to specific blaKPC gene variants dictates the need for applicable diagnostic methods for allele discrimination. We report here a simple method of blaKPC-9 allele recognition based on a combination of endonuclease digestion analysis and PCR amplification using unique primers. K. pneumoniae isolates carrying the blaKPC gene were tested. Digestion with RsaI restriction endonuclease was found to efficiently differentiate the blaKPC-2 from the blaKPC-9 variants into two distinct groups of digestion patterns named KPC-2-like and KPC-9-like, respectively. An additional procedure, the amplification refractory mutation system (ARMS) method, was applied to identify the variant within the same group. The principles of this procedure could be developed to identify several blaKPC gene variants, as well as monitoring the spread and evolution of specific KPC variants within local geographical regions.
Prevalence of JP2 and Non-JP2 Genotypes of Aggregatibacter actinomycetemcomitans and Oral Hygiene Practice of Kenyan Adolescents in Maasai Mara
Authors: Haubek Dorte , Mulli Tonnie , Kemoli Arthur , Lindholm Mark , Gjørup Hans , Nørregaard Marie-Louise Milvang , Johansson Anders
Published: April 2021

Aggregatibacter actinomycetemcomitans is implicated in the etiology of periodontitis that affects adolescents. The monitoring and mapping of the geographic dissemination pattern of JP2 and non-JP2 genotypes of A. actinomycetemcomitans are of interest. In Africa, the highly leukotoxic JP2 genotype is known to be prevalent, particularly in north-west Africa. The aims of this study were to determine the prevalence of JP2 and non-JP2 genotypes and investigate the oral hygiene practices among adolescents living in Maasai Mara, Kenya. A total of 284 adolescents (mean age: 15.0 yrs; SD 1.1) were interviewed regarding their age, gender, medical history, and oral hygiene practice, and the number of teeth present was recorded. One subgingival pooled plaque sample from all the first molars of each participant was analyzed by conventional PCR. The mean number of permanent teeth present was 27.9 (SD: 2.0; range: 22–32; 95% CI: 27.7–28.1). Sixteen (5.6%) and two (0.7%) adolescents were positive for non-JP2 and JP2 genotypes, respectively. For the vast majority of the adolescents, the use of a toothbrush (99.3%) and toothpaste (80.1%), as well as some kind of toothpick (>60.2%), were part of their oral hygiene practice, with dental floss (0.4%) and/or mouth rinses (0.4%) rarely being used. We have, for the first time, identified Kenyan adolescents colonized with the JP2 genotype. The prevalence of the JP2 genotype of A. actinomycetemcomitans is low, a possible indicator that it spreading through human migration from North and West Africa to East Africa is a rare occasion.
Efficacy and Safety of Azelaic Acid Nanocrystal-Loaded In Situ Hydrogel in the Treatment of Acne Vulgaris
Authors: Tomić Ivona , Miočić Sandra , Pepić Ivan , Šimić Dubravka , Filipović-Grčić Jelena
Published: April 2021

Acne vulgaris is a common, multifactorial, inflammatory skin disease affecting the pilosebaceous unit. Topical therapy is the first choice in the treatment of mild to moderate acne, and azelaic acid (AZA) is one of the most commonly used drugs. The aim of this study was to evaluate the safety and efficacy of a low-dose azelaic acid nanocrystal (AZA-NC) hydrogel in the treatment of mild to moderate facial acne. The study was designed as a double-blind, randomized controlled trial. Patients were randomized to treatment with AZA-NC hydrogel, 10%, or AZA cream, 20%, administered in quantities of approximately 1 g twice daily for 8 weeks. Efficacy of therapy was measured by the number of lesions and safety by the frequency and severity of adverse events. At week 8, the success rate of treatment with AZA-NC hydrogel, 10%, was 36.51% (p < 0.001) versus 30.37% (p < 0.001) with AZA cream. At week 8, treatment with AZA-NC hydrogel, 10%, resulted in a significant reduction in total inflammatory lesions from baseline of 39.15% (p < 0.001) versus 33.76% (p < 0.001) with AZA cream, and a reduction in non-inflammatory lesions from baseline of 34.58% (p < 0.001) versus 27.96% (p < 0.001) with AZA cream, respectively. The adverse event rate was low and mostly mild.
Study on the Nanosensor Based on a MIM Waveguide with a Stub Coupled with a Horizontal B-Type Cavity
Authors: Yan Shubin , Shi Haoran , Yang Xiaoyu , Guo Jing , Wu Wenchang , Hua Ertian
Published: April 2021

Due to their compact size and high sensitivity, plasmonic sensors have become a hot topic in the sensing field. A nanosensor structure, comprising the metal–insulator–metal (MIM) waveguide with a stub and a horizontal B-Type cavity, is designed as a refractive index sensor. The spectral characteristics of proposed structure are analyzed via the finite element method (FEM). The results show that there is a sharp Fano resonance profile, which is excited by a coupling between the MIM waveguide and the horizontal B-Type cavity. The normalized HZ field is affected by the difference value between the outer radii R1 and R2 of the semi-circle of the horizontal B-Type cavity greatly. The influence of every element of the whole system on sensing properties is discussed in depth. The sensitivity of the proposed structure can obtain 1548 nm/RIU (refractive index unit) with a figure of merit of 59. The proposed structure has potential in nanophotonic sensing applications.
Phase Compensation Method in OPA System Based on the Linear Electro-Optic Effect
Authors: Yang Shuaishuai , Liu Dean
Published: April 2021

Factors such as mechanical deformation and temperature changes lead to phase mismatch in optical parametric amplification systems, impacting energy stability. A phase compensation method via the linear electro-optic effect can overcome this limitation. Phase mismatch compensation characteristics were simulated via the linear electro-optic effect in 70%-deuterated DKDP and 95%-deuterated DKDP. This method was applied to OPA systems to verify its feasibility. The results show that the temperature acceptance bandwidth of 70%-deuterated DKDP and 95%-deuterated DKDP can be ~1.75 and ~2 times larger, respectively, than that of the OPA without compensation. Moreover, the angle acceptance bandwidth of 70%-deuterated DKDP and 95%-deuterated DKDP can be ~2 times larger than that of the OPA without compensation. The abovementioned method can facilitate the compensation of phase mismatch within a range and can be widely used in OPA and optical parametric chirped pulse amplification systems to improve laser stability.
A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts
Authors: Moon Ki-Beom , Park Ji-Sun , Park Su-Jin , Lee Hyo-Jun , Cho Hye-Sun , Min Sung-Ran , Park Youn-Il , Jeon Jae-Heung , Kim Hyun-Soon
Published: April 2021

Both obtaining high-yielding, viable protoplasts and following reliable regeneration protocols are prerequisites for the continuous expansion and development of newly emerging systems involving protoplast utilization. This study determines an efficient process from protoplast isolation to shoot regeneration in vitro. The maximum yield of protoplast extraction, which was 6.36 ± 0.51 × 106 protoplasts/g fresh weight (FW), was approximately 3.7 times higher than that previously reported for potato protoplasts. To obtain data, wounded leaves were used by partially cutting both sides of the midrib, and isolated protoplasts were purified by the sucrose cushion method, with a sucrose concentration of 20%. We confirmed a significant effect on the extraction efficiency by measuring enzymolysis during a 6 h period, with three times more washing buffer than the amount normally used. Protoplasts fixed in alginate lenses with appropriate space were successfully recovered and developed into microcalli 2 weeks after culture. In addition, to induce high efficiency regeneration from protoplasts, calli in which greening occurred for 6 weeks were induced to develop shoots in regeneration medium solidified by Gelrite, and they presented a high regeneration efficiency of 86.24 ± 11.76%.
Biochemical and Proteomic Changes in the Roots of M4 Grapevine Rootstock in Response to Nitrate Availability
Authors: Prinsi Bhakti , Muratore Chiara , Espen Luca
Published: April 2021

In agricultural soils, nitrate (NO3−) is the major nitrogen (N) nutrient for plants, but few studies have analyzed molecular and biochemical responses involved in its acquisition by grapevine roots. In viticulture, considering grafting, NO3− acquisition is strictly dependent on rootstock. To improve the knowledge about N nutrition in grapevine, this study analyzed biochemical and proteomic changes induced by, NO3− availability, in a hydroponic system, in the roots of M4, a recently selected grapevine rootstock. The evaluation of biochemical parameters, such as NO3−, sugar and amino acid contents in roots, and the abundance of nitrate reductase, allowed us to define the time course of the metabolic adaptations to NO3− supply. On the basis of these results, the proteomic analysis was conducted by comparing the root profiles in N-starved plants and after 30 h of NO3− resupply. The analysis quantified 461 proteins, 26% of which differed in abundance between conditions. Overall, this approach highlighted, together with an increased N assimilatory metabolism, a concomitant rise in the oxidative pentose phosphate pathway and glycolysis, needed to fulfill the redox power and carbon skeleton demands, respectively. Moreover, a wide modulation of protein and amino acid metabolisms and changes of proteins involved in root development were observed. Finally, some results open new questions about the importance of redox-related post-translational modifications and of NO3− availability in modulating the dialog between root and rhizosphere.
Inhibitory Effects of Brassicaceae Cover Crop on Ambrosia artemisiifolia Germination and Early Growth
Authors: Šćepanović Maja , Sarić-Krsmanović Marija , Šoštarčić Valentina , Brijačak Ema , Lakić Josip , Špirović Trifunović Bojana , Gajić Umiljendić Jelena , Radivojević Ljiljana
Published: April 2021

Several cover crops (CCs) exert allelopathic effects that suppress weed growth. The aim of the present study was to evaluate the effects of aqueous extracts containing different concentrations [0, 0.5, 1, 2.5, 5, 7.5 and 10% (w/v)] of Brassicaceae CCs (Sinapis alba, Raphanus sativus, Camellina sativa) and of the CCs Fagopyrum esculentum and Guizotia abyssinica on germination and early growth of Ambrosia artemisiifolia L. The allelopathic effects were species and concentration-dependent. C. sativa, for example, caused the greatest potential to inhibit germination, shoot, radicle length and fresh seedling weight, whereas S. alba and R. sativus inhibited germination and early growth of A. artemisiifolia only at concentrations ≥7.5%. In contrast, no inhibition was observed when aqueous extracts of F. escultneum and G. abyssinica were added at any of tested concentration. Liquid chromatography-tandem mass spectrometry detected 15 phenolic compounds in Brassicaceae CCs with the highest content (µg/g) of vanillin (48.8), chlorogenic acid (1057), vanilic acid (79), caffeic acid (102.5) and syringic acid (27.3) in C. sativa. Our results suggest that C. sativa is the most allelopathic CCs and that the fruits of C. sativa are the plant organs richest in allelochemicals.
Application of Redox-Responsive Hydrogels Based on 2,2,6,6-Tetramethyl-1-Piperidinyloxy Methacrylate and Oligo(Ethyleneglycol) Methacrylate in Controlled Release and Catalysis
Authors: Khodeir Miriam , Jia He , Vlad Alexandru , Gohy Jean-François
Published: April 2021

Hydrogels have reached momentum due to their potential application in a variety of fields including their ability to deliver active molecules upon application of a specific chemical or physical stimulus and to act as easily recyclable catalysts in a green chemistry approach. In this paper, we demonstrate that the same redox-responsive hydrogels based on polymer networks containing 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) stable nitroxide radicals and oligoethylene glycol methyl ether methacrylate (OEGMA) can be successfully used either for the electrochemically triggered release of aspirin or as catalysts for the oxidation of primary alcohols into aldehydes. For the first application, we take the opportunity of the positive charges present on the oxoammonium groups of oxidized TEMPO to encapsulate negatively charged aspirin molecules. The further electrochemical reduction of oxoammonium groups into nitroxide radicals triggers the release of aspirin molecules. For the second application, our hydrogels are swelled with benzylic alcohol and tert-butyl nitrite as co-catalyst and the temperature is raised to 50 °C to start the oxidation reaction. Interestingly enough, benzaldehyde is not miscible with our hydrogels and phase-separate on top of them allowing the easy recovery of the reaction product and the recyclability of the hydrogel catalyst.
Double-Function Oxygen Scavenger and Aromatic Food Packaging Films Based on LDPE/Polybutadiene and Peanut Aroma
Authors: Juan-Polo Adriana , Maestre Pérez Salvador E. , Monedero Prieto María , Tone Ana María , Sánchez Reig Carmen , Beltrán Sanahuja Ana
Published: April 2021

The aim of this study was to develop a double function active packaging material for nuts. The packaging solution, on the one hand, integrated polybutadiene (PB) as an oxygen scavenger and, on the other hand, it incorporated peanut aroma (PA) to improve customer’s sensorial experience. Different formulations based on low density polyethylene (LDPE), commercial PA (5 wt %) and PB at two levels (5 wt % and 13 wt %) were obtained by cast film extrusion. The obtained films were compared in terms of their mechanical, structural, optical and thermal properties confirming a plasticizing effect of PA and PB resulting in an increase in the ductility of the polymer and in a slight decrease in the thermal properties, maintaining their transparency. Regarding the oxygen capacity of the films, values of 4.4 mL and 2.7 mL O2 g−1 film were obtained for PE/PA/PB13 and PE/PA/PB5, respectively, after 6 days proving the suitability of the UV irradiation treatment in improving the oxygen absorption capacity of PB without the need of a metal catalyst. The aroma retention capacity into the polymer matrix was also evaluated in the developed formulations. The incorporation of PB in 13 wt % into a LDPE matrix improved the PA retention. This behavior was attributed to the ability of PB in enhancing cross-linking of LDPE as the concentration of PB increases. The results suggested the potential of PE/PB/PB13 films as oxygen scavenger and aromatic food packaging system to offer protection against lipid oxidation in nuts.
Starch-Based Super Water Absorbent: A Promising and Sustainable Way to Increase Survival Rate of Trees Planted in Arid Areas
Authors: Lertsarawut Pattra , Rattanawongwiboon Thitirat , Tangthong Theeranan , Laksee Sakchai , Kwamman Tanagorn , Phuttharak Butri , Romruensukharom Phayao , Suwanmala Phiriyatorn , Hemvichian Kasinee
Published: April 2021

This research aimed to scale up the production of starch-based super water absorbent (SWA) and to validate the practical benefits of SWA for agricultural applications. SWA was successfully prepared in an up-scaling production by radiation-induced graft polymerization of acrylic acid onto cassava starch. Chemical characterization by FTIR and thermal characterization by TGA showed results that differentiated starting materials from the prepared SWA, thus confirming effective preparation of starch-based SWA via radiation-induced graft polymerization. SEM results visibly revealed a highly porous morphology of the synthesized SWA, substantiating its high swelling ability. Results from the field tests, performed for two seasons, revealed that the prepared SWA was able to increase the survival rate of young rubber trees planted in arid area by up to 40%, while simultaneously enhancing the growth characteristics of the young rubber trees.
Structure and Properties of Octenyl Succinic Anhydride-Modified High-Amylose Japonica Rice Starches
Authors: Zhang Wei , Cheng Bei , Li Jiahui , Shu Zaixi , Wang Pingping , Zeng Xuefeng
Published: April 2021

Starches rich in amylose are promising functional ingredients for calory-reduced foods. In this research, a high-amylose Japonica rice starch (amylose content 33.3%) was esterified with octenyl succinic anhydride (OSA) to improve the functional properties. The OSA-modified derivatives were evaluated for structure and functional properties, with OSA-modified normal Japonica rice starch (amylose content 18.8%) used as control. Fourier transform infrared spectra confirmed the introduction of OSA groups to starch. OSA modification made little change to morphology and particle size of high-amylose starch, but decreased the relative crystallinity and pasting temperature and increased the pasting viscosity, swelling power, emulsifying stability, and resistant starch (RS) content. The changes of properties were related to the degree of substitution (DS). Typically, OSA-modified high-amylose starch at DS of 0.0285 shows polyhedral-shape granules, with a volume-average particle diameter of 8.87 μm, peak viscosity of 5730 cp, and RS content of 35.45%. OSA-modified high-amylose starch had greater peak viscosity and RS content and lower swelling power than OSA-modified normal starch of similar DS, but the two kinds of derivatives did not have a significant difference in emulsifying stability. The OSA-modified high-amylose Japonica rice starch could be used as an emulsifier, thickener, and fat replacer in food systems.
Polylactic Acid (PLA) Biocomposite: Processing, Additive Manufacturing and Advanced Applications
Authors: Ilyas R.A. , Sapuan S.M. , Harussani M.M. , Hakimi M.Y.A.Y. , Haziq M.Z.M. , Atikah M.S.N. , Asyraf M.R.M. , Ishak M.R. , Razman M.R. , Nurazzi N.M. , Norrrahim M.N.F. , Abral Hairul , Asrofi Mochamad
Published: April 2021

Over recent years, enthusiasm towards the manufacturing of biopolymers has attracted considerable attention due to the rising concern about depleting resources and worsening pollution. Among the biopolymers available in the world, polylactic acid (PLA) is one of the highest biopolymers produced globally and thus, making it suitable for product commercialisation. Therefore, the effectiveness of natural fibre reinforced PLA composite as an alternative material to substitute the non-renewable petroleum-based materials has been examined by researchers. The type of fibre used in fibre/matrix adhesion is very important because it influences the biocomposites’ mechanical properties. Besides that, an outline of the present circumstance of natural fibre-reinforced PLA 3D printing, as well as its functions in 4D printing for applications of stimuli-responsive polymers were also discussed. This research paper aims to present the development and conducted studies on PLA-based natural fibre bio-composites over the last decade. This work reviews recent PLA-derived bio-composite research related to PLA synthesis and biodegradation, its properties, processes, challenges and prospects.
Preparation and Characterization of Nanofibrous Scaffolds of Ag/Vanadate Hydroxyapatite Encapsulated into Polycaprolactone: Morphology, Mechanical, and In Vitro Cells Adhesion
Authors: El-Hamshary Hany , El-Naggar Mehrez E. , El-Faham Ayman , Abu-Saied M. A. , Ahmed M. K. , Al-Sahly Mosaed
Published: April 2021

Series of nanofibrous composites of polycaprolactone (PCL) were fabricated in different compositions of modified hydroxyapatite (HAP). The encapsulated HAP was co-doped with Ag/vanadate ions at different Ag contributions. XRD and FTIR techniques confirmed the powder and fibrous phase formation. Further, the morphological and mechanical behaviors of the electrospun nanofibrous scaffolds containing hydroxyapatite were investigated. The nanofibrous phases were biologically evaluated via studying contact angle, antibacterial, cell viability, and in vitro growth of human fibroblasts cell line (HFB4). It is obvious that silver ions cause gradual deviation in powder grains from wafer-like to cloudy grains. The maximum height of the roughness (Rt) ranged from 902.0 to 956.9 nm, while the valley depth of the roughness (Rv) ranged from 308.3 to 442.8 nm, for the lowest and the highest additional Ag ions for powdered phases. Moreover, the highest contribution of silver through the nanofibrous phases leads to the formation of lowest filaments size ranged from 0.07 to 0.53 µm. Further, the fracture strength was increased exponentially from 2.51 ± 0.35 MPa at zero concentration of silver ions up to 4.23 ± 0.64 MPa at 0.6 Ag/V-HAP@PCL. The fibrous phases were biologically evaluated in terms of antibacterial, cell viability, and in vitro growth of human fibroblasts cell line (HFB4). The nanofibrous composition of 0.8 Ag/V-HAP@PCL reached the maximum potential against E. coli and S. aureus and recorded 20.3 ± 1.1 and 19.8 ± 1.2 mm, respectively. This significant performance of the antibacterial activity and cell viability of co-doped HAP distributed through PCL could recommend these compositions for more research in biological applications, including wound healing.
Recycling and Utilization of Polymers for Road Construction Projects: An Application of the Circular Economy Concept
Authors: Anwar Muhammad Kashif , Shah Syyed Adnan Raheel , Alhazmi Hatem
Published: April 2021

Numerous environmental issues arise as a result of a linear economy strategy: reserves become scarce and end up in landfills and as greenhouse gases. Utilizing waste as a resource or shifting towards a circular economy are among the effective strategies for addressing these issues. To track this shift, appropriate measures that concentrate on sustainable development while taking practical contexts into consideration are required. In this paper, we utilize plastic wastes as a replacement for bitumen for reuse aiming at a circular economy. The use of plastic waste materials, i.e., plastic bottles (PET) and gas pipes (PE) in asphalt materials as a bitumen modifier was studied through series of experimental lab test methods. Marshall samples were prepared using a conventional Marshall method containing five different percentages (0%, 5%, 10%, 15%, and 20%) of plastic content by total weight of bitumen. Samples were tested after 1 and 30 days and the result shows that the stability of plastic-modified asphalt concrete was increased after 30 days, while still meeting standard criteria with plastic contents up to 20%. Moreover, the addition of waste plastic in road construction is a very effective strategy for reusing plastic waste, which also provides economic and social benefits for a sustainable approach to road pavements.
Statistical Optimization of Biodiesel Production from Salmon Oil via Enzymatic Transesterification: Investigation of the Effects of Various Operational Parameters
Authors: Ramakrishnan Vegneshwaran V. , Dave Deepika , Liu Yi , Routray Winny , Murphy Wade
Published: April 2021

The enzymatic transesterification of Atlantic salmon (Salmo salar) oil was carried out using Novozym 435 (immobilized lipase from Candida antartica) to produce biodiesel. A response surface modelling design was performed to investigate the relationship between biodiesel yield and several critical factors, including enzyme concentration (5, 10, or 15%), temperature (40, 45, or 50 °C), oil/alcohol molar ratio (1:3, 1:4, or 1:5) and time (8, 16, or 24 h). The results indicated that the effects of all the factors were statistically significant at p-values of 0.000 for biodiesel production. The optimum parameters for biodiesel production were determined as 10% enzyme concentration, 45 °C, 16 h, and 1:4 oil/alcohol molar ratio, leading to a biodiesel yield of 87.23%. The step-wise addition of methanol during the enzymatic transesterification further increased the biodiesel yield to 94.5%. This is the first study that focused on Atlantic salmon oil-derived biodiesel production, which creates a paradigm for valorization of Atlantic salmon by-products that would also reduce the consumption and demand of plant oils derived from crops and vegetables.
Impact of Binary Chemical Reaction and Activation Energy on Heat and Mass Transfer of Marangoni Driven Boundary Layer Flow of a Non-Newtonian Nanofluid
Authors: Punith Gowda Ramanahalli Jayadevamurthy , Naveen Kumar Rangaswamy , Jyothi Anigere Marikempaiah , Prasannakumara Ballajja Chandrappa , Sarris Ioannis E.
Published: April 2021

The flow and heat transfer of non-Newtonian nanofluids has an extensive range of applications in oceanography, the cooling of metallic plates, melt-spinning, the movement of biological fluids, heat exchangers technology, coating and suspensions. In view of these applications, we studied the steady Marangoni driven boundary layer flow, heat and mass transfer characteristics of a nanofluid. A non-Newtonian second-grade liquid model is used to deliberate the effect of activation energy on the chemically reactive non-Newtonian nanofluid. By applying suitable similarity transformations, the system of governing equations is transformed into a set of ordinary differential equations. These reduced equations are tackled numerically using the Runge–Kutta–Fehlberg fourth-fifth order (RKF-45) method. The velocity, concentration, thermal fields and rate of heat transfer are explored for the embedded non-dimensional parameters graphically. Our results revealed that the escalating values of the Marangoni number improve the velocity gradient and reduce the heat transfer. As the values of the porosity parameter increase, the velocity gradient is reduced and the heat transfer is improved. Finally, the Nusselt number is found to decline as the porosity parameter increases.
Effect of Drying on Nutritional Composition of Atlantic Sea Cucumber (Cucumaria frondosa) Viscera Derived from Newfoundland Fisheries
Authors: Liu Yi , Dave Deepika , Trenholm Sheila , Ramakrishnan Vegneshwaran V. , Murphy Wade
Published: April 2021

Cucumaria frondosa is the main sea cucumber species harvested from Newfoundland waters. During processing, the viscera of sea cucumber are usually discarded as waste. As a matter of fact, sea cucumber viscera are abundant in various nutrients and promising for valorization. In the present study, sea cucumber viscera were pretreated by air drying and freeze drying, and the nutritional compositions of the dried products were investigated, including proximate composition, lipid class, fatty acid profile, and amino acid composition. The dried viscera had similar levels of ash, lipids, and proteins compared to fresh viscera. Both air- and freeze-dried viscera had total fatty acid composition similar to fresh viscera, with high levels of omega-3 polyunsaturated fatty acids (PUFAs) (30–31%), especially eicosapentaenoic acid (27–28%), and low levels of omega-6 PUFAs (~1%). The dried samples were abundant in essential amino acids (46–51%). Compared to air-dried viscera, freeze-dried viscera contained a lower content of moisture and free fatty acids, and higher content of glycine and omega-3 PUFAs in phospholipid fraction. The high content of nutritious components in dried viscera of Cucumaria frondosa indicates their great potential for valorization into high-value products.
Hybrid Process Models in Electrochemical Syntheses under Deep Uncertainty
Authors: Francis-Xavier Fenila , Kubannek Fabian , Schenkendorf René
Published: April 2021

Chemical process engineering and machine learning are merging rapidly, and hybrid process models have shown promising results in process analysis and process design. However, uncertainties in first-principles process models have an adverse effect on extrapolations and inferences based on hybrid process models. Parameter sensitivities are an essential tool to understand better the underlying uncertainty propagation and hybrid system identification challenges. Still, standard parameter sensitivity concepts may fail to address comprehensive parameter uncertainty problems, i.e., deep uncertainty with aleatoric and epistemic contributions. This work shows a highly effective and reproducible sampling strategy to calculate simulation uncertainties and global parameter sensitivities for hybrid process models under deep uncertainty. We demonstrate the workflow with two electrochemical synthesis simulation studies, including the synthesis of furfuryl alcohol and 4-aminophenol. Compared with Monte Carlo reference simulations, the CPU-time was significantly reduced. The general findings of the hybrid model sensitivity studies under deep uncertainty are twofold. First, epistemic uncertainty has a significant effect on uncertainty analysis. Second, the predicted parameter sensitivities of the hybrid process models add value to the interpretation and analysis of the hybrid models themselves but are not suitable for predicting the real process/full first-principles process model’s sensitivities.
A Proper Shape of the Trailing Edge Modification to Solve a Housing Damage Problem in a Gas Turbine Power Plant
Authors: Jansaengsuk Thodsaphon , Kaewbumrung Mongkol , Busayaporn Wutthikrai , Thongsri Jatuporn
Published: April 2021

To solve the housing damage problem of a fractured compressor blade (CB) caused by an impact on the inner casing of a gas turbine in the seventh stage (from 15 stages), modifications of the trailing edge (TE) of the CB have been proposed, namely 6.5 mm curved cutting and a combination of 4 mm straight cutting with 6.5 mm curved cutting. The simulation results of the modifications in both aerodynamics variables Cl and Cd and the pressure ratio, including structural dynamics such as a normalized power spectrum, frequency, total deformation, equivalent stress, and the safety factor, found that 6.5 mm curved cutting could deliver the aerodynamics and structural dynamics similar to the original CB. This result also overcomes the previous work that proposed 5.0 mm straight cutting. This work also indicates that the operation of a CB gives uneven pressure and temperature, which get higher in the TE area. The slightly modified CB can present the difference in the properties of both the aerodynamics and the structural dynamics. Therefore, any modifications of the TE should be investigated for both properties simultaneously. Finally, the results from this work can be very useful information for the modification of the CB in the housing damage problem of the other rotating types of machinery in a gas turbine power plant.
A Process for Carbon Dioxide Capture Using Schiff Bases Containing a Trimethoprim Unit
Authors: Yaseen Anaheed A. , Al-Tikrity Emaad T. B. , El-Hiti Gamal A. , Ahmed Dina S. , Baashen Mohammed A. , Al-Mashhadani Mohammed H. , Yousif Emad
Published: April 2021

Environmental problems associated with the growing levels of carbon dioxide in the atmosphere due to the burning of fossil fuels to satisfy the high demand for energy are a pressing concern. Therefore, the design of new materials for carbon dioxide storage has received increasing research attention. In this work, we report the synthesis of three new Schiff bases containing a trimethoprim unit and the investigation of their application as adsorbents for carbon dioxide capture. The reaction of trimethoprim and aromatic aldehydes in acid medium gave the corresponding Schiff bases in 83%–87% yields. The Schiff bases exhibited surface areas ranging from 4.15 to 20.33 m2/g, pore volumes of 0.0036–0.0086 cm3/g, and average pore diameters of 6.64–1.4 nm. An excellent carbon dioxide uptake (27–46 wt%) was achieved at high temperature and pressure (313 K and 40 bar, respectively) using the Schiff bases. The 3-hydroxyphenyl-substituted Schiff base, which exhibited a meta-arrangement, provided the highest carbon dioxide uptake (46 wt%) due to its higher surface area, pore volume, and pore diameter compared with the other two derivatives with a para-arrangement.
Ripple Attenuation for Induction Motor Finite Control Set Model Predictive Torque Control Using Novel Fuzzy Adaptive Techniques
Authors: Zhang Zhihui , Wei Hongyu , Zhang Wei , Jiang Jianan
Published: April 2021

Finite control set model predictive torque control (FCS-MPTC) strategy has been widely used in induction motor (IM) control due to its fast response characteristic. Although the dynamics of the FCS-MPTC method are highly commended, its steady-state performance—ripple deserves attention in the meantime. To improve the steady-state performance of the IM drives, this paper proposes an improved FCS-MPTC strategy, based on a novel fuzzy adaptive speed controller and an adaptive weighting factor, tuning strategy to reduce the speed, torque and flux ripples caused by different factors. Firstly, a discrete predicting plant model (PPM) with a new flux observer is established, laying the ground for achieving an FCS-MPTC algorithm accurately. Secondly, after analyzing the essential factors in establishing a fuzzy adaptive PI controller, with high ripple suppression capacity, an improved three-dimensional controller is designed. Simultaneously, the implementation procedures of the fuzzy adaptive PI controller-based FCS-MPTC are presented. Considering that a weighting factor must be employed in the cost function of an FCS-MPTC method, system ripples increase if the value of the weighting factor is inappropriate. Then, on that basis, a novel fuzzy adaptive theory-based weighting factor tuning strategy is proposed, with the real-time torque and flux performance balanced. Finally, both simulation and hardware-in-loop (HIL) test are conducted on a 1.1 kW IM drive to verify the proposed ripple reduction algorithms.
Analysis of the Physical Properties of Seeds of Selected Viburnum Species for the Needs of Seed Sorting Operations
Authors: Kaliniewicz Zdzisław , Choszcz Dariusz J.
Published: April 2021

Viburnum is a genus of colorful and ornamental plants popular in landscape design on account of their high esthetic appeal. The physical properties of viburnum seeds have not been investigated in the literature to date. Therefore, the aim of this study was to characterize the seeds of selected Viburnum species and to search for potential relationships between their physical attributes for the needs of seed sorting operations. The basic physical parameters of the seeds of six Viburnum species were measured, and the relationships between these attributes were determined in correlation and regression analyses. The average values of the evaluated parameters were determined in the following range: terminal velocity—from 5.6 to 7.9 m s−1, thickness—from 1.39 to 1.87 mm, width—from 3.59 to 6.33 mm, length—from 5.58 to 7.44 mm, angle of external friction—from 36.7 to 43.8°, mass—from 16.7 to 35.0 mg. The seeds of V. dasyanthum, V. lentago and V. sargentii should be sorted in air separators, and the seeds of V. lantana and V. opulus should be processed with the use of mesh screens with round apertures to obtain uniform size fractions. The seeds of V. rhytodophyllum cannot be effectively sorted into batches with uniform seed mass, but they can be separated into groups with similar dimensions.
Revealing of Supercritical Water Gasification Process of Lignin by Reactive Force Field Molecular Dynamics Simulations
Authors: Ponnuchamy Veerapandian , Sandak Jakub , Sandak Anna
Published: April 2021

Gasification with supercritical water is an efficient process that can be used for the valorization of biomass. Lignin is the second most abundant biopolymer in biomass and its conversion is fundamental for future energy and value-added chemicals. In this paper, the supercritical water gasification process of lignin by employing reactive force field molecular dynamics simulations (ReaxFF MD) was investigated. Guaiacyl glycerol-β-guaiacyl ether (GGE) was considered as a lignin model to evaluate the reaction mechanism and identify the components at different temperatures from 1000 K to 5000 K. The obtained results revealed that the reactions and breaking of the lignin model started at 2000 K. At the primary stage of the reaction at 2000 K the β-O-4 bond tends to break into several compounds, forming mainly guaiacol and 1,3-benzodioxole. In particular, 1,3-benzodioxole undergoes dissociation and forms cyclopentene-based ketones. Afterward, dealkylation reaction occurred through hydroxyl radicals of water to form methanol, formaldehyde and methane. Above 2500 K, H2, CO and CO2 are predominantly formed in which water molecules contributed hydrogen and oxygen for their formation. Understanding the detailed reactive mechanism of lignin’s gasification is important for efficient energy conversion of biomass.
Analysis of the Surface Subsidence Induced by Mining Near-Surface Thick Lead-Zinc Deposit Based on Numerical Simulation
Authors: Zhao Yifan , Zhao Xingdong , Dai Jiajia , Yu Wenlong
Published: April 2021

This paper describes a case study of surface subsidence in the Hongling Lead-Zinc Mine. Hongling Lead-Zinc Mine is located in Inner Mongolia, China, about 240 km away from the border between China and Mongolia. There is a batch of outcrops of the near-surface thick steep-dip metamorphic orebody. The large-scale surface subsidence induced by underground excavation has brought some impact on the safety of herdsmen and their daily husbandry activities nearby. The requirements of reclamation for subsidence areas in the relevant laws and regulations, raise enormous pressure and risk on safe and economic operation. In this paper, a 3D numerical model of this mine was built by 3DMine and FLAC3D to analyse the excavation procedure and mechanism. The results of the simulation were in good agreement with the field subsidence data collected by satellites and unmanned aerial vehicles from 2009 to 2019. The analysis showed that the current mining method—an integrated underground method of stoping and caving—accelerated the surface subsidence, and some measures of monitoring, controlling and management were expected to take in order to improve economic and ecological benefits.
Effect of Gas Diffusion Layer Thickness on the Performance of Anion Exchange Membrane Fuel Cells
Authors: Truong Van Men , Duong Ngoc Bich , Yang Hsiharng
Published: April 2021

Gas diffusion layers (GDLs) play a critical role in anion exchange membrane fuel cell (AEMFC) water management. In this work, the effect of GDL thickness on the cell performance of the AEMFC was experimentally investigated. Three GDLs with different thicknesses of 120, 260, and 310 µm (denoted as GDL-120, GDL-260, and GDL-310, respectively) were prepared and tested in a single H2/O2 AEMFC. The experimental results showed that the GDL-260 employed in both anode and cathode electrodes exhibited the best cell performance. There was a small difference in cell performance for GDL-260 and GDL-310, while water flooding was observed in the case of using GDL-120 operated at current densities greater than 1100 mA cm−2. In addition, it was found that the GDL thickness had more sensitivity to the AEMFC performance as used in the anode electrode rather than in the cathode electrode, indicating that water removal at the anode was more challenging than water supply at the cathode. The strategy of water management in the anode should be different from that in the cathode. These findings can provide a further understanding of the role of GDLs in the water management of AEMFCs.
Multi-Objective Nonlinear Programming Model for Reducing Octane Number Loss in Gasoline Refining Process Based on Data Mining Technology
Authors: Liu Xiao , Liu Yilai , He Xuejun , Xiao Min , Jiang Tao
Published: April 2021

To simultaneously reduce automobile exhaust pollution to the environment and satisfy the demand for high-quality gasoline, the treatment of fluid catalytic cracking (FCC) gasoline is urgently needed to minimize octane number (RON) loss. We presented a new systematic method for determining an optimal operation scheme for minimising RON loss and operational risks. Firstly, many data were collected and preprocessed. Then, grey correlative degree analysis and Pearson correlation analysis were used to reduce the dimensionality, and the major variables with representativeness and independence were selected from the 367 variables. Then, the RON and sulfur (S) content were predicted by multiple nonlinear regression. A multi-objective nonlinear optimization model was established with the maximum reduction in RON loss and minimum operational risk as the objective function. Finally, the optimal operation scheme of the operating variable corresponding to the sample with a RON loss reduction greater than 30% in 325 samples was solved in Python.
Robots, Extinction, and Salvation: On Altruism in Human–Posthuman Interactions
Authors: Odorčák Juraj , Bakošová Pavlína
Published: April 2021

Posthumanism and transhumanism are philosophies that envision possible relations between humans and posthumans. Critical versions of posthumanism and transhumanism examine the idea of potential threats involved in human–posthuman interactions (i.e., species extinction, species domination, AI takeover) and propose precautionary measures against these threats by elaborating protocols for the prosocial use of technology. Critics of these philosophies usually argue against the reality of the threats or dispute the feasibility of the proposed measures. We take this debate back to its modern roots. The play that gave the world the term “robot” (R.U.R.: Rossum’s Universal Robots) is nowadays remembered mostly as a particular instance of an absurd apocalyptic vision about the doom of the human species through technology. However, we demonstrate that Karel Čapek assumed that a negative interpretation of human–posthuman interactions emerges mainly from the human inability to think clearly about extinction, spirituality, and technology. We propose that the conflictual interpretation of human–posthuman interactions can be overcome by embracing Čapek’s religiously and philosophically-inspired theory of altruism remediated by technology. We argue that this reinterpretation of altruism may strengthen the case for a more positive outlook on human–posthuman interactions.
Recovering the Irrecoverable: Blackness, Melancholy, and Duplicities That Bind
Authors: Winters Joseph
Published: April 2021

In this article, I critically engage Stephen Best’s provocative text, None Like Us. The article agrees with Best’s general concerns regarding longings for a unified black community or a We before the collective crime of slavery. Yet I contend that melancholy, which Best associates with black studies’ desire to recover a lost object, can be read in a different direction, one that includes both attachment and wound, investment and dissolution. To think with and against Best, I examine Spike Lee’s School Daze in conversation with Freud, Benjamin, and Morrison.