bluuebunny/crossref_metadata_2025 huggingface dataset:
Phylotranscriptomics reveals the reticulate evolutionary history of a widespread diatom species complex
Authors: Çiftçi Ozan , Alverson Andrew J. , van Bodegom Peter , Roberts Wade R. , Mertens Adrienne , Van de Vijver Bart , Trobajo Rosa , Mann David G. , Pirovano Walter , van Eijk Iris , Gravendeel Barbara
Published: July 2022

In contrast to surveys based on a few genes that often provide limited taxonomic resolution, transcriptomes provide a wealth of genomic loci that can resolve relationships among taxonomically challenging lineages. Diatoms are a diverse group of aquatic microalgae that includes important bioindicator species and many such lineages. One example is Nitzschia palea, a widespread species complex with several morphologically defined taxonomic varieties, some of which are critical pollution indicators. Morphological differences among the varieties are subtle and phylogenetic studies based on a few genes fail to resolve their evolutionary relationships. We conducted morphometric and transcriptome analyses of 10 Nitzschia palea strains to resolve the relationships among strains and taxonomic varieties. Nitzschia palea was resolved into three clades, one of which corresponds to a group of strains with narrow linear‐lanceolate valves. The other morphological group recovered in the shape outline analysis was not monophyletic and consisted of two clades. Gene‐tree concordance analyses and phylogenetic network estimations revealed patterns of incomplete lineage sorting and gene flow between intraspecific lineages. We detected reticulated evolutionary patterns among lineages with different morphologies, resulting in a putative recent hybrid. Our study shows that phylogenomic analyses of unlinked nuclear loci, complemented with morphometrics, can resolve complex evolutionary histories of recently diverged species complexes.
Uncertainty quantification of structural flexibility identified from input–output measurement data for reliability analysis
Authors: Li Panjie , Zhao Tongkuai , Zhang Jian , Wei Jiabei , Feng Maria Q. , Feng Dongming , Li Shengli
Published: July 2022

In contrast to the traditional uncertainty quantification of the modal parameters identified from the output‐only measurement data, this study develops an uncertainty propagation and quantification method for structural flexibility identified from input–output measurement data and then uses the derived flexibility matrix for structural reliability analysis. First, a novel procedure for variance estimations of the complete system matrix of the state‐space model is derived based on first‐order perturbation theory. Then, the mode scaling factors and integrated modal flexibility are subsequently derived and quantified. Finally, the predicted displacements and their uncertainty intervals are used to implement the reliability analysis in terms of the structural displacement serviceability limit state (SLS). The accuracies of the derived expressions are verified by a numerical example through the comparison of variance values estimated from Monte Carlo simulations. The simulation example also indicates that we can expect to identify structural parameters with higher reliability if the input force to the structure is measured, and the identified modal scaling and corresponding flexibility matrix undergo more uncertainty than those of the modal parameters. Finally, we apply the proposed uncertainty quantification method to a scaled three‐story frame structure to demonstrate its practicability. The proposed method bridges a gap between the identified structural flexibility and structural reliability analysis directly by the probability category.
Molecular investigation of the incidence of Candida auris infections at selected hospitals in Iran
Authors: Safari Fatemeh , Madani Mahboobeh , Kheirollahi Majid , Mirhendi Hossein
Published: July 2022

Background

The accurate occurrence rate of C. auris infections is still not clear, mainly due to the defects in detection and identification tools routinely used. In this study, we used conventional PCR and real‐time PCR assays for sensitive and specific detection/identification of C. auris from either yeast isolates or clinical specimens collected from various patients in different parts of Iran. Our survey is the first large‐scale study rating the incidence of C. auris infections in Iran.

Methods

A total of 439 yeast isolates and 590 clinical specimens were screened by specific C. auris‐PCR, targeting the ITS region. The validity of positive samples was assessed by sequencing.

Results

Four out of 590 clinical specimens (0.68%) were positive by conventional PCR, while in real‐time PCR performed on 100 clinical samples, including those four samples positive in conventional samples, 6 samples were positive. A complete agreement of the identification of positive cases with sequencing results was documented. Among 439 culture isolates, none was positive for C. auris. After following up and resampling of the patients with positive PCR, only one specimen showed positive culture for C. auris, which was confirmed by sequencing.

Conclusion

C. auris is not a common cause of systemic or superficial fungal infections in Iran, and a few detected positive cases can be considered as a commensal, coloniser or infecting yeast which may potentially emerge in some clinical and therapeutical conditions. Mycological and phenotypical assays are not sensitive approaches for isolation/identification of C. auris, unless a specific and sensitive molecular‐based method is applied.
Evidence of chromatin and transcriptional dynamics for cold development in peach flower bud
Authors: Canton Monica , Forestan Cristian , Marconi Gianpiero , Carrera Esther , Bonghi Claudio , Varotto Serena
Published: July 2022

In temperate zones, fruit trees regulate their annual growth cycle to seasonal environmental changes. During the cold season, growth is limited by both environmental and genetic factors. After the exposure to low temperature and fulfillment of chilling requirements, mild temperatures promote the growth and flowering. However, an insufficient chilling exposure may lead to nonuniform blooming, with a negative impact on fruit set.

To gain insights into flower development in the fruit tree buds, peach is an interesting model, the flower and vegetative bud being distinct organs. To understand how flower bud development is regulated, we integrated cytological observations and epigenetic and chromatin genome‐wide data with transcriptional changes to identify the main regulatory factors involved in flower development during chilling accumulation.

We demonstrated that growth cessation does not occur in peach flower buds during chilling accumulation, but that there are changes in transcript abundance of key genes of hormone metabolism and flower bud development, distribution of histone modifications (H3K4me3 and H3K27me3) and DNA methylation.

Altogether, our findings indicate that during the cold season the flower bud is in a nondormant state and that the chilling experience allows flower differentiation to be completed.
Analysis of Th‐cell subsets in local and systemic environments from experimental periodontitis rats
Authors: Yuan Yun , Zhang Hongming , Gu Qinhua , Xu Xinrui , Yu Runping , Huang Hui
Published: July 2022

Objectives: The objective of this study was to explore the effect of periodontitis on Th‐cell subsets in local and systemic environments.

Methods: A total of 32 male Sprague‒Dawley rats were randomly divided into periodontitis and control groups. Silk ligatures were applied to the mandibular first (M1) molars in the periodontitis group. Inflammation and alveolar bone loss around the M1 molars were analyzed by histological staining and microcomputed tomography. The mRNA expression of interferon‐γ (IFN‐γ), interleukin 4 (IL‐4), IL‐17, and IL‐10 in the gingiva was measured by qRT‐PCR. The proportions of Th1, Th2, Th17, and Treg cells in the submandibular lymph nodes, peripheral blood, and jaw bone marrow were tested using flow cytometry.

Results: More inflammatory cells and alveolar bone resorption were found in the periodontitis group, with upregulated mRNA expression of IFN‐γ, IL‐17, and IL‐10. The proportion of Th1 and Th17 cells was significantly elevated in submandibular lymph nodes, and the proportion of Th1, Th2, and Th17 cells was significantly elevated in peripheral blood, while the proportion of Th1, Th17, and Treg cells was significantly elevated in jaw bone marrow in the periodontitis group.

Conclusion: This study suggests that periodontitis affects the differentiation of Th‐cell subsets in both local and systemic environments, resulting in an increased proportion of proinflammatory cells.
A reappraisal of microbiome dysbiosis during experimental periodontitis
Authors: Arce Marion , Endo Natalia , Dutzan Nicolas , Abusleme Loreto
Published: July 2022

Periodontitis is a chronic inflammatory disease associated with the presence of dysbiotic microbial communities. Several studies interrogating periodontitis pathogenesis have utilized the murine ligature‐induced periodontitis (LIP) model and have further examined the ligature‐associated microbiome relying on 16S rRNA‐based sequencing techniques. However, it is often very challenging to compare microbial profiles across studies due to important differences in bioinformatic processing and databases used for taxonomic assignment. Thus, our study aim was to reanalyze microbiome sequencing datasets from studies utilizing the LIP model through a standardized bioinformatic analysis pipeline, generating a comprehensive overview of microbial dysbiosis during experimental periodontitis.We conducted a reanalysis of 16S rDNA gene sequencing datasets from nine published studies utilizing the LIP model. Reads were grouped according to the hypervariable region of the 16S rDNA gene amplified (V1‐V3 and V4), preprocessed, binned into operational taxonomic units and classified utilizing relevant databases. Alpha‐ and beta‐diversity analyses were conducted, along with relative abundance profiling of microbial communities.

Our findings revealed similar microbial richness and diversity across studies and determined shifts in microbial community structure determined by periodontitis induction and study of origin. Clear variations in the relative abundance of bacterial taxa were observed starting on day 5 after ligation and onward, consistent with a distinct microbial composition during health and experimental periodontitis. We also uncovered differentially represented bacterial taxa across studies, dominating periodontal health and LIP‐associated communities.

Collectively, this reanalysis provides a unified overview of microbial dysbiosis during the LIP model, providing new insights that aim to inform further studies dedicated to unraveling oral host–microbial interactions.
Protein interactome mapping of Porphyromonas gingivalis provides insights into the formation of the PorQ‐Z complex of the type IX secretion system
Authors: Gorasia Dhana G. , Veith Paul D. , Reynolds Eric C.
Published: July 2022

Porphyromonas gingivalis is an anaerobic Gram‐negative human oral pathogen highly associated with the more severe forms of periodontal disease. Porphyromonas gingivalis utilises the type IX secretion system (T9SS) to transport ∼30 cargo proteins, including multiple virulence factors, to the cell surface. The T9SS is a multiprotein system consisting of at least 20 proteins, and recently, we characterised the protein interactome of these components. Similar to the T9SS, almost all biological processes are mediated through protein‒protein interactions (PPIs). Therefore, mapping PPIs is important to understand the biological functions of many proteins in P. gingivalis. Herein, we provide native migration profiles of over 1000 P. gingivalis proteins. Using the T9SS, we demonstrate that our dataset is a useful resource for identifying novel protein interactions. Using this dataset and further analysis of T9SS P. gingivalis mutants, we discover new mechanistic insights into the formation of the PorQ‐Z complex of the T9SS. This dataset is a valuable resource for studies of P. gingivalis.
Successful treatment of a child's pityriasis rubra pilaris (PRP) with ustekinumab and acitretin
Authors: Katharina Medek , Sylvia Selhofer , Matthias Buchner , Ulrich Mrowietz , Martin Laimer
Published: July 2022

Pityriasis rubra pilaris (PRP) is a rare inflammatory skin disease that occurs with phenotypic variability in adults of all ages as well as in children. Data on the treatment of PRP is limited. Here, we report a 5‐year‐old girl with widespread skin involvement and prominent palmoplantar hyperkeratosis who was initially treated for psoriasis. After reevaluation, a diagnosis of PRP was made, and the patient had an excellent therapeutic response to ustekinumab and acitretin.
Livedo racemosa associated with central venous catheter use in a newborn
Authors: Barsan Kaya Tuğba , Kaya Erdoğan Hilal , Güneş Damla , Aydemir Özge
Published: July 2022

Livedo racemosa (LRac) refers to violaceous, red, or blue netlike mottling of the skin in an irregular pattern caused by circulatory abnormalities. Central venous catheters (CVC) are widely used in neonates who require intensive care to offer steady intravenous access. CVCs are covered with a hydrophilic polymer gel that reduces friction between the sheath and the vessel wall, thereby minimizing vascular spasm, irritation, and aneurysm formation. However, the introduction of foreign material into the vein carries a risk of embolization. Recently, LRac was reported as a cutaneous manifestation of hydrophilic polymer gel embolization (HPE) in adults. We present a case of LRac in a newborn that developed following CVC insertion and resolved spontaneously after the catheter was removed.
CD7 CAR T bridging to allo‐HSCT in R/R T‐ALL: A case report
Authors: Fanqiao Meng , Chen Xiuqiong , Ren Xiaotong , Li Lijuan , Wu Tong
Published: July 2022

Background

Refractory/relapsed T‐cell acute lymphoblastic leukemia (R/R T‐ALL) is a hematological malignancy with a poor prognosis. The current treatment strategy has not benefited most patients, and the treatment methods are still being explored.

Case presentation

An 8‐year‐old boy with R/R T‐ALL achieved CR after multiple chemotherapies, followed by the first allo‐HSCT. Unfortunately, 1 year and 3 months later, he relapsed. After recurrence, the patient underwent multiple chemotherapies, but the NOTCH1 gene and MRD were still positive. FCM and immunohistochemistry revealed abnormally high expression of CD7, so we considered bridging the second allo‐HSCT after CD7 CAR T‐cells treatment. The patient has low toxic and side effects and is still in CR, findings from this case report have more important therapeutic significance for R/R T‐ALL.

Conclusion

In conclusion, CD7 CAR T‐cells bridging to allo‐HSCT is a safe and effective approach for R/R T‐ALL, resulting in durable CR and longer survival.
THE NEGATIVELY BIASED MANDARIN BELIEF VERB yĭwéi*
Authors: Glass Lelia
Published: July 2022

Abstract

The Mandarin belief verb yĭwéi strongly suggests that the belief it embeds is wrong or questionable. Based on original data, I propose that this sense of negative bias stems from a postsupposition that the reported belief must not be accepted in the Common Ground following an update with yĭwéi. When a belief is reported using a neutral, nonfactive verb such as rènwéi ‘think,’ it is possible for the content of that belief to become Common Ground if the belief or belief‐holder are considered reliable; but the postsupposition of yĭwéi explicitly blocks such an inference, giving rise to its negative bias. By heading off a potential inference, this postsupposition is further argued to serve a function common to other proposed postsuppositions in the literature. Zooming out, this investigation illuminates the complex calculations triggered by belief reports in discourse, and the linguistic resources used to guide them.
Calcification and osseous metaplasia of the meibomian glands of a horse
Authors: Gunsalus Kerry , Taylor Ryan , Croix Noelle La
Published: July 2022

Objective

To report the clinical history, surgical management, and histologic findings of meibomian gland calcification and osseous metaplasia in a horse.

Animal Studied

A 21‐year‐old Selle Français gelding presented with a 9 months history of blepharitis, blepharospasm, and epiphora affecting the right eye. The horse was diagnosed with meibomianitis and impaction with associated granulomas, but not treated surgically for 6 years.

Procedures

Physical and ophthalmic examinations, and systemic bloodwork were performed. A diamond burr debridement was performed on a corneal ulceration, and meibomian gland nodules were excised and examined histologically.

Results

Multiple firm concretions were associated with the palpebral conjunctiva of each eyelid. The largest nodule (5 × 4 × 10 mm) was excised from the lower right eyelid. Histology revealed dilated meibomian ducts with mineralized inspissated secretions surrounded by fibrosis and osseous metaplasia. Following nodule excision and corneal ulcer resolution, no blepharospasm or epiphora recurred for a follow‐up period of 15 months.

Conclusion

Calcification and osseous metaplasia of the meibomian glands was identified in a Selle Français gelding. It is suspected that inspissated meibomian secretions led to a local granulomatous reaction with secondary dystrophic calcification and osseous metaplasia.
Laparoscopic vertical sleeve gastrectomy in felines: A cadaveric feasibility study and experimental case series in two cats
Authors: Buote Nicole J. , Porter Ian , Loftus John , Cummings Bethany , Dakin Gregory F.
Published: July 2022

Objective

To evaluate the feasibility of laparoscopic vertical sleeve gastrectomy (LVSG) in feline cadavers using endoscopic stapling equipment and report clinical outcomes in two live feline subjects.

Study design

Cadaveric study and experimental case series.

Animals

Ten feline cadavers; two feline subjects.

Methods

LVSG technique was refined on feline cadavers and included retraction of the liver, dissection of the stomach, assessment of proper location for gastrectomy via stapling, and leak testing. Appropriateness of gastrectomy, gastrectomy %, surgical times and complications were recorded. The procedure was performed on two live feline subjects, and they were followed for 4 months to report surgical complications.

Results

LVSG was completed in 9/10 cadavers and both live patients. Stenosis at the incisura was recorded in 2/9 cadavers. No obvious leaks were seen in the 8 cadavers that were tested or either live patient. The mean surgical time for all cadaver procedures and live patients was 110.4 and 115 minutes, respectively. Mean weight of resected cadaver stomach was 10 g and the mean % of the total stomach weight resected was 27.6%. No intra‐ or postoperative surgical complications occurred in the live subjects.

Conclusion

LVSG technique appears feasible and safe for use in live patients.

Clinical relevance: This LVSG technique may be safely used for partial gastric resection in cats. Further studies are necessary to determine if it is effective at reversing the effects of obesity and diabetes in this population.
Mitogenomics supports the monophyly of Mysidacea and Peracarida (Malacostraca)
Authors: Höpel Christoph G. , Yeo Darren , Grams Markus , Meier Rudolf , Richter Stefan
Published: July 2022

Here, we provide the first complete mitochondrial genomes for two higher taxa of Peracarida, Lophogastrida and Stygiomysida. We examined Lophogaster typicus as a representative of Lophogastrida and Spelaeomysis bottazzii as a representative of Stygiomysida. Both mitogenomes have all typical metazoan genes (13 protein‐coding genes, two ribosomal RNA genes and 22 transfer RNAs). The mitogenomes have a length of 15,076 bp in L. typicus and 14,806 bp in S. bottazzii. Gene order differs markedly from the hypothetical pancrustacean/malacostracan ground pattern in both species, and in L. typicus, all genes were encoded on the heavy strand. This is the first time this is described for a crustacean. We also reconstruct eumalacostracan phylogenies using a data set consisting of 98 species based on alignments comprising all protein‐encoding genes as well as the protein‐encoding genes and the two ribosomal RNAs. We find support for the monophyly of Mysidacea based on species from all three higher taxa (Mysida, Lophogastrida, Stygiomysida). Moreover, our analyses also support a monophyletic Peracarida with Amphipoda or Amphipoda + Mysidacea as the sister group to the remaining Peracarida.
Exercise and hypoxia unmask pulmonary vascular disease and right ventricular dysfunction in a 10‐ to 12‐week‐old swine model of neonatal oxidative injury
Authors: Steenhorst Jarno J. , Hirsch Alexander , Verzijl Annemarie , Wielopolski Piotr , de Wijs‐Meijler Daphne , Duncker Dirk J. , Reiss Irwin K. M. , Merkus Daphne
Published: July 2022

Prematurely born young adults who experienced neonatal oxidative injury (NOI) of the lungs have increased incidence of cardiovascular disease. Here, we investigated the long‐term effects of NOI on cardiopulmonary function in piglets at the age of 10–12 weeks. To induce NOI, term‐born piglets (1.81 ± 0.06 kg) were exposed to hypoxia (10–12% ), within 2 days after birth, and maintained for 4 weeks or until symptoms of heart failure developed (range 16–28 days), while SHAM piglets were normoxia raised. Following recovery (>5 weeks), NOI piglets were surgically instrumented to measure haemodynamics during hypoxic challenge testing (HCT) and exercise with modulation of the nitric‐oxide system. During exercise, NOI piglets showed a normal increase in cardiac index, but an exaggerated increase in pulmonary artery pressure and a blunted increase in left atrial pressure – suggesting left atrial under‐filling – consistent with an elevated pulmonary vascular resistance (PVR), which correlated with the duration of hypoxia exposure. Moreover, hypoxia duration correlated inversely with stroke volume (SV) during exercise. Nitric oxide synthase inhibition and HCT resulted in an exaggerated increase in PVR, while the PVR reduction by phosphodiesterase‐5 inhibition was enhanced in NOI compared to SHAM piglets. Finally, within the NOI piglet group, prolonged duration of hypoxia was associated with a better maintenance of SV during HCT, likely due to the increase in RV mass. In conclusion, duration of neonatal hypoxia appears an important determinant of alterations in cardiopulmonary function that persist further into life. These changes encompass both pulmonary vascular and cardiac responses to hypoxia and exercise.

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Key points

Children who suffered from neonatal oxidative injury, such as very preterm born infants, have increased risk of cardiopulmonary disease later in life.

Risk stratification requires knowledge of the mechanistic underpinning and the time course of progression into cardiopulmonary disease.

Exercise and hypoxic challenge testing showed that 10‐ to 12‐week‐old swine that previously experienced neonatal oxidative injury had increased pulmonary vascular resistance and nitric oxide dependency.

Duration of neonatal oxidative injury was a determinant of structural and functional cardiopulmonary remodelling later in life.

Remodelling of the right ventricle, as a result of prolonged neonatal oxidative injury, resulted in worse performance during exercise, but enabled better performance during the hypoxic challenge test.

Increased nitric oxide dependency together with age‐ or comorbidity‐related endothelial dysfunction may contribute to predisposition to pulmonary hypertension later in life.
Molecular determinants underlying volume‐regulated anion channel subunit‐dependent oxidation sensitivity
Authors: Bertelli Sara , Zuccolini Paolo , Gavazzo Paola , Pusch Michael
Published: July 2022

The volume‐regulated anion channel (VRAC) is formed by LRRC8 subunits. Besides their role in the maintenance of cell homeostasis, VRACs are critically involved in oxidative stress mechanisms: reactive oxygen species directly modulate VRACs in a subunit‐dependent manner. It was reported that LRRC8A–LRRC8E heteromeric channels are activated by oxidation, whereas LRRC8A–LRRC8C heteromers are inhibited. Here we adopted chimeric‐ as well as concatemeric‐based strategies to identify residues responsible for the divergent effect of oxidants. We identified two cysteines in the first two leucine rich repeats of LRRC8E, C424 and C448, as the targets of oxidation. Oxidation likely results in the formation of a disulfide bond between the two cysteines, which in turn induces a conformational change leading to channel activation. Additionally, we found that LRRC8C inhibition is caused by oxidation of the first methionine. We thus identified crucial molecular elements involved in channel activation, which are conceivably relevant in determining physiological ROS effects.

image

Key points

Volume‐regulated anion channels (VRACs) are heterohexameric complexes composed of an essential LRRC8A subunit and a variable number of LRRC8B–E subunits.

VRACs are directly regulated by oxidation, with LRRC8A–LRRC8E heteromers being potentiated and LRRC8A–LRRC8C heteromers being inhibited by oxidation.

We identified two LRRC8E specific intracellular cysteines that form a disulfide bond upon oxidation leading to LRRC8A–LRRC8E potentiation.

Inhibition of LRRC8A–LRRC8C heteromers is mediated by the oxidation of the start methionine, being additionally dependent on the identity of the LRR domain.

Besides providing physiological insights concerning the outcome of reactive oxygen species modulation, the results point to key structural elements involved in VRAC activation.
Facilitation of the lordosis reflex of female rats from the ventromedial nucleus of the hypothalamus.
Authors: Pfaff D W , Sakuma Y
Published: July 2022

1. Effects of electrical stimulation of hypothalamic ventromedial nucleus (v.m.n.) on the lordosis reflex of female rats were examined in ovariectomized and oestrogen‐primed animals with chronically implanted electrodes. 2. Lordosis triggered either by manual cutaneous stimulation or by male mounting, was facilitated by electrical stimulation of the v.m.n. 3 A gradual increase in lordosis performance followed a relatively long period of stimulation; never less than 15 min and usually about 1 hr of stimulation was necessary for maximum facilitation. Following the termination of stimulation, the performance returned gradually to the control level during a 5‐‐8 hr period. 4. The optimal frequency of stimulation was between 10 and 30 Hz. Threshold for effective facilitation was, on the average, 12.5 microA. 5. Stimulation tended to induce larger facilitation when applied to the lateral side of v.m.n. 6. Pre‐treatment with oestrogen was necessary to obtain facilitation by v.m.n. stimulation. The threshold dosage of oestrogen was 2.5 microgram per animal. 7. Stimulation was effective in adrenalectomized rats, in dexamethasone‐primed animals, and in rats pre‐treated with exogenous progesterone. Thus, adrenal prodesterone release is not required for the v.m.n. facilitation of lordosis. 8. Medial preoptic stimulation with the same parameters suppressed the lordosis reflex. 9. The v.m.n. participates in the control of lordosis by a facilitatory output. The delay before facilitation implies that the v.m.n. is not in the direct reflex‐arc for the execution of lordosis. Rather, a summation or interaction process with an unusually long time course is involved.
La renuncia del papa y la sede apostólica vacante
Authors:   , Torres Velásquez Wilinton Camilo
Published: July 2022

Esta investigación se ha desarrollado en tres capítulos: En el primer capítulo se ha ilustrado histórica y canónicamente, la institución de la renuncia del Papa y sus correspondientes implicaciones al quedar la Sede Vacante. En el capítulo dos se estudia desde el ámbito canónico la provisión y pérdida de los oficios en la Iglesia con sus consecuencias y la definición y descripción de los términos análogos de la renuncia. En el capítulo tres hacemos un recorrido histórico de las fuentes del Canon 332 § 2 describiendo las notas para que la renuncia sea considerada como válida, al igual que el procedimiento a seguir cuando la Sede quede vacante.
El departamento o consejo de conciliación de los actos administrativos en el derecho canónico : propuesta tutelar de los derechos subjetivos
Authors:   , Gutiérrez Motta Leivi Leonardo
Published: July 2022

En el primer capítulo de esta investigación se presentan los antecedentes reflexivos que dieron origen al este nuevo canon en la legislación eclesial (Can. 1733 s2), a su vez, se expone el desarrollo de los antecedentes doctrinales y judiciales. En el segundo capítulo se presenta una investigación realizada acerca de la concreción o no de esta propuesta legislativa y pastoral en las Conferencias del Episcopado Latinoamericano y del Caribe para determinar la existencia de este Departamento. Y en el tercer capítulo, se plantean los principios operacionales y prácticos como propuesta metodológica para constituir un determinado Departamento o Consejo de Conciliación en una Conferencia Episcopal, que permita ejecutar la facultad concedida por el Can. 1733 s2.
La dispensa matrimonial rato no consumado : una acción pastoral del derecho canónico
Authors:   , Jiménez Díaz Guillermo Segundo
Published: July 2022

Esta investigación se ha desarrollado en tres capítulos: El primer capítulo hace un recorrido histórico jurídico de la dispensa en la Iglesia que nos muestra la evolución del concepto y la normativa desde su origen hasta la formulación actual en el Código vigente. El segundo capítulo nos habla sobre el estudio de la dispensa, haciéndonos un comparativo de! matrimonio rato y no consumado en los Códigos de derecho Canónico, partiendo del contexto de la dispensa en general, mostrándonos la delimitación de este tema dentro del Código como también el desarrollo de su proceso jurídico, tanto como la autoridad competente En el tercer capítulo se presenta una fundamentación del aspectos pastoral y salvífico de la dispensa matrimonial rato y no consumado, a través de los distintos documentos magisteriales de la Iglesia que hacen referencia al sacramento del matrimonio, la familia, la indisolubilidad y la alianza. La Metodología que se empleó en la elaboración de esta investigación fue basada en la metodología histórica, comparativa, y explorativa, a través y por medio de estas directrices, se ha llegado a una sene de conclusiones, que nos permiten descubrir el papel de la iglesia entre los fieles como un ente Jurídico pastoral de gran mediación y atención de sus miembros, que a lo largo de la historia viene estructurándose y fortaleciendo su ordenación jurídica.
¿Existe una relación directa entre la nulidad matrimonial consagrada en el canon 1095,2 del C.I.C. y la imposibilidad de creación de un juicio crítico? : una propuesta que responde a la necesidad inminente de una actuación pastoral de acuerdo con el canon 1063,2 del C.I.C, para una celebración válida y lícita. (1066 del C.I.C.)
Authors:   , Salamanca de Moreno Clemencia
Published: July 2022

"La presente investigación doctoral, se fundamenta en el análisis de dos discursos del Santo Padre (Juan Pablo II y Benedicto XVI) a la Sacra Rota Romana, uno el 18 de enero de 1990, y el otro de especial importancia, pronunciado el 22 de enero de 2011. La pertinencia del trabajo, radica en el urgente llamado del Papa, para que se observe especial cuidado en la preparación al matrimonio, explicando la estrecha relación entre derecho y pastoral, y la necesidad apremiante de una preparación inmediata al sacramento del matrimonio (1063,2), que permita conocer los requisitos canónicos a los contrayentes, como prevención de la nulidad matrimonial, particularmente por la causal consagrada en el canon 1095,2, ante la realidad que se presenta a los contrayentes, ""hijos de su tiempo"", por las ideologías reinantes que han sido denunciadas por los últimos cuatro Pontífices y que impiden construir un juicio crítico, de acuerdo con el plan de Dios para el matrimonio que permita consentir válidamente. Se presenta como propuesta ante la Conferencia Episcopal de Colombia, entre los que se destacan el concepto de amor como decisión, el sentido de la cruz y la importancia de la gracia santificante, en forma adicional al conocimiento de lo que significa la alianza matrimonial, los fines y las propiedades del sacramento matrimonial."
La potestad de régimen en el oficio del vicario parroquial
Authors:   , Amaya González Neil
Published: July 2022

El presente trabajo indaga acerca de la potestad de régimen en el oficio del Vicario parroquial: ¿qué especie de potestad de régimen detenta? ¿ordinaria vicaria? ¿delegada? Para responder a estos interrogantes el trabajo se ha organizado en tres partes: la primera trata de la potestad de régimen en general, es decir, su origen, el sujeto que la posee, el fin y su distinción en las funciones legislativa, judicial y ejecutiva; la segunda parte presenta el oficio de Vicario parroquial a través de su evolución histórica hasta llegar al actual Código de Derecho Canónico; finalmente, la tercera parte, a partir del contenido de las dos primeras, trata de argumentar como la potestad de régimen del Vicario parroquial es ordinaria vicaria.
Situación jurídica del clérigo, por abandono del ministerio sacerdotal y pérdida del estado clerical
Authors:   , Coronado Moreno Wilson
Published: July 2022

En la vida del sacerdote, en su labor pastoral, se integran algunos sentimientos como la soledad y la búsqueda del afecto humano que, en múltiples circunstancias, le obligan a dejar el ministerio. En ocasiones, algunos viven sin resolver su situación jurídica, ya sea porque no piden el debido proceso y orientación o porque desconocen el recurso de la Dispensa de sus obligaciones, por la pérdida del Estado Clerical y el abandono de su Ministerio Sacerdotal.
Las penas expiatorias. Finalidad, alcance y aplicación : análisis jurídico - pastoral cánones 1336-1338
Authors:   , Barrios Baldovino Juan Rafael
Published: July 2022

La Iglesia como sociedad viva y renovada permanentemente, tiene potestad para sancionar a sus miembros, lo que busca cuando tiene que sancionar a uno de ellos cuando ha incurrido en un delito contra el orden establecido: Es reparar el daño cometido, garantizar la disciplina eclesiástica, el arrepentimiento del delincuente, sobre todo su conversión y la salvación de su alma. Las penas expiatorias como instrumento jurídico-coactivo garantizan no solo el castigo del delincuente, evitando la impunidad, sino que su finalidad es más ontológica; garantizar una justicia eficaz, el bienestar y la armonía de la comunidad Eclesiástica, es decir, la plena comunión. La Iglesia siempre aplicará la pena con equidad canónica y con espíritu de pastoralidad porque su fin supremo es la salus animarum.
Aportes de un gran maestro al derecho matrimonial canónico : jurisprudencia de los canones 1905, 2 y 3. Padre Aldo Stella Ibáñez
Authors:   , Echeverría Pulido Jorge Elías
Published: July 2022

La Universidad Javeriana de Bogotá, queriendo hacer memoria de sus más egregios profesores, ha desplegado varias iniciativas, entre ellas, la aprobación de esta monografía, en honor más que merecido, del Sacerdote de la Arquidiócesis de Bogotá, Padre Aldo Stella Ibáñez, para lo cual confluyeron, la voluntad del Decano de Derecho Canónico, los aportes muy valiosos, y profundos del Doctor Rafael Gómez Betancur, quien compartió por lo menos un lustro con el P. Aldo, en un ambiente de sana emulación intelectual por los temas e jurídicos, que como en el caso del canon 1095, exigían erudición y soporte de ciencias como la Psiquiatría y Psicología, que eran el bastión de nuestro gran maestro, y de otra parte, la voluntad de este servidor para sacar el proyecto y hacerlo una realidad al lado de nuestro tutor. El trabajo busca presentar, a través de un caso concreto, la grave falta de discreción de juicio acerca de los derechos y deberes esenciales del matrimonio que mutuamente se han de dar y aceptar (1095,2) y de quienes no pueden asumir las obligaciones esenciales del matrimonio por causas de naturaleza psíquica (1095,3). Además de presentar el perfil de nuestro sabio educador Aldo Stella, tomamos una de las sentencias de él en un caso de alcoholismo crónico, donde hace gala de su genialidad y sapiencia como juez ponderado, fiel a los principios canónicos. Sin duda que los aportes en las diversas ramas del saber son la base de este trabajo monográfico.
Estatuto jurídico del catecúmeno : una propuesta para la iglesia colombiana a la luz de la exigencia del canon 788 del Código de Derecho Canónico de 1983
Authors:   , Arias Jiménez Carlos Guillermo
Published: July 2022

Este trabajo es una propuesta de elaboración del Estatuto Jurídico del Catecúmeno para la Iglesia colombiana, con el propósito de que pueda responder a las exigencias del canon 788 del Código de Derecho Canónico de 1983. Para este propósito se presentan tres capítulos ordenados de la siguiente manera:
El catecúmeno en el magisterio conciliar del Vaticano II y en la legislación canónica
Los catecúmenos en algunas Conferencias Episcopales de América a la luz del canon 788
Elementos para la elaboración del Estatuto del Catecúmeno para Colombia
La potestad ejecutiva o administrativa del vicario general en la iglesia particular
Authors:   , Pineda Ospina Wilmar Alonso
Published: July 2022

El capítulo primero contiene una detallada presentación de la potestad vicaria en los orígenes bíblicos y eclesiológicos, cuyo método histórico evidencia la forma en que esa potestad está íntimamente ligada al plan divino de la salvación que, a su vez, se amalgama entre la tradición eclesial y la legislación canónica.
En el capítulo segundo se dedica al estudio de la potestad vicaria, específicamente a la potestad ejecutiva o administrativa del vicario general en la Iglesia diocesana, mediante un método comparativo que aborda: la Sagrada Escritura, el magisterio eclesial y los cánones de la legislación establecida en los códigos de Derecho Canónico de 1917 y 1983.
En el capítulo tercero, el método exegético permite indagar cuál es el origen del vicario general, en la estructura jerárquica, los requisitos para su nombramiento, sus funciones en el ejercicio de la potestad encomendada como participación, en la capitalidad del obispo diocesano, y quienes, en derecho, se equiparan a él (cfr. can. 368).
El sigilo sacramental ante las leyes estatales que obligan a la denuncia de un delito
Authors:   , Soler Rodríguez Wilson Eduardo
Published: July 2022

El presente trabajo busca analizar el sigilo sacramental ante la posibilidad, y en algunos casos, ya una realidad, de que el Estado, en ejercicio de su deber jurisprudencial, determine por peso de ley, obligar a los ministros ordenados católicos a quebrantar el sigilo sacramental ante el conocimiento de un delito cometido por un penitente y revelado en celebración del sacramento de la penitencia.
Por un lado, se encuentra la conciencia y la confianza del penitente ante el confesor que le atiende sacramentalmente, confía plenamente en la responsabilidad ética y espiritual del ministro ante todo lo que le será revelado y colocado, en sigilo, a su cuidado.
Por otro lado, se ve la tendencia, cada vez más creciente de algunos Estados, de legislar para que, en algunos casos, el sigilo pueda ser dispensado cuando el penitente deba responder ante la justicia civil por alguna denuncia grave en su contra.
Análisis de las técnicas de reproducción humana asistida desde el punto de vista canónico y jurídico
Authors:   , Yllarramendy Crespo Rebeca
Published: July 2022

El análisis de las técnicas de reproducción humana asistida es un estudio sistemático sobre las técnicas modernas de reproducción humana, el cual se realizó haciendo un repaso de cada técnica científica y de su correspondiente crítica desde el magisterio eclesiástico con algunas consideraciones filosóficas, teológicas y jurídicas, con miras a la formación de una conciencia clara para las parejas que se encuentran en situación de esterilidad y/o infertilidad. El objetivo final es crear un discurso desde el derecho canónico estableciendo las posibles normas que deben cobijar a los fieles cristianos con relación a las TRA.
Diagnosis of Water Failures in Proton Exchange Membrane Fuel Cells via Physical Parameter Resistances of the Fractional Order Model and Fast Fourier Transform Electrochemical Impedance Spectroscopy
Authors: Arama Fatima Zohra , Laribi Slimane , Mammar Khaled , Aoun Nouar , Ghaitaoui Touhami , Hamouda Messaoud
Published: July 2022

The identification of water status is the foundation for fuel cell water management, which is helpful to fuel cell reliability and longevity. In this article, a novel and reliable method for diagnosing the hydration condition of proton exchange membrane fuel cells (PEMFCs) was presented using a fractional-order model (FOM) to represent the PEMFCs impedance. The results show that the mean root-mean-squared error (RMSE) and mean absolute percentage error (MAPE) between the proposed model and experimental data (in normal, drying, or flooding cases) are about 0.034 and 0.473, respectively. The fast Fourier transform–electrochemical impedance spectroscopy technique (FFT-EIS) was used as an alternative technique that is simple and efficient to electrochemical impedance spectroscopy (EIS). The PEMFCs hydration state is monitored by observing the changing effect of the physical resistor values (membrane resistance, polarization, and diffusion resistances) of the proposed model. These resistors, characterized by their high sensitivity to the drying and flooding of PEMFCs, affect the Nyquist impedance spectra and frequency spectrum amplitudes at low and high frequencies. Based on the obtained results, it is concluded that the proposed strategy can be used to develop new domains in which the PEMFCs’ hydration states can be properly predicted.
Effective Diffusivity From Analytical Solution for Banks of Cylinders
Authors: Beale S. B.
Published: July 2022

Benchmarks are provided for the evaluation of the effective coefficient for species diffusion, or electrical/thermal conduction, in structured porous media. The cases considered corresponding to doubly periodic rows of circular cylinders, for which a power series solution has been previously obtained, from complex variable theory. Both inline and staggered geometries are considered for three common configurations: inline-square, rotated-square, and equilateral geometries. From these mathematical solutions, values for the effective conduction/diffusion coefficient are readily constructed. The results are presented in terms of correlations for the ratio of effective-to-bulk conductivity/diffusivity or microstructural parameter, as a function of porosity. It is shown that near identical results with the present analytical analysis are obtained using calculations based on a finite-volume method and also with a previous mathematical analysis for the case of inline-square geometry. The present analytical solutions are also compared with two well-known correlations for random porous media, based on effective medium and percolation theory. It is shown that agreement with the analytical solution is not in general particularly good and depends on the choice of fitting parameters. The present results may be used as canonical data for comparative studies with numerical procedures to enumerate microstructural parameters for arbitrary-shaped occlusions in random geometries.
Seasonal abundance of ticks collected at US Army Garrison Humphreys, Pyeongtaek, Republic of Korea, 2019–2021
Authors: Chong Sung-Tae , Kim Heung-Chul , Kim Myung-Soon , Mahabir Sanjeev , A. Klein Terry , G. Robbins Richard
Published: July 2022

Tick surveillance was conducted annually from February–November 2019–2021 at US Army Garrison (USAG) Humphreys, Pyeongtaek, Republic of Korea (ROK). Three general habitats, cut grasses, uncut grasses + herbaceous vegetation, and young mixed (deciduous and conifer) forest groves, were surveyed using tick drags. Ticks also were collected from Korean water deer, Hydropotes inermis argyropus, killed by vehicles at USAG Humphreys. A total of 53,428 ticks (18,004, 16,921, and 18,503 during 2019–2021, respectively) belonging to two genera and five species were collected by tick drag. Haemaphysalis longicornis (48,880; 91.49%) was the most frequently collected tick, followed by H. flava (2,501; 4.68%), Ixodes nipponensis (2,019; 3.78%), H. phasiana (27; 0.05%), and H. japonica (1; <0.01%). A total of 1,340 ticks belonging to two genera and three species were collected from 20/27 (74.07%) Korean water deer from January–December 2019–2021. Haemaphysalis longicornis (1,138; 84.93%) was the most commonly collected tick, followed by I. nipponensis (184; 13.73%), and H. flava (18; 1.34%). Ixodes nipponensis was collected from Korean water deer throughout the year, indicating that they were active during all seasons in the ROK. Haemaphysalis longicornis adults and nymphs were collected similarly from all habitats during March-November. Haemaphysalis flava was more frequently collected during the early and late seasons when there were relatively low temperatures. Unlike other species, I. nipponensis was collected more frequently in mixed forests from March-June. Adult male H. longicornis accounted for only 0.05% (3♂/5,769♀) of the total number of adults collected by tick drag, while no males (0♂/194♀) were collected from Korean water deer. In contrast, only H. flava males (15) were collected from Korean water deer, while both sexes of I. nipponensis (103♂/75♀) were collected during 2019–2021.
Two new species of Neocarus (Acari: Opilioacaridae) from Piauí and Rio de Janeiro, Brazil, with remarks on integument and seta shape as characters
Authors: De Araújo Marcel Santos , Di Palma Antonella , Ochoa Ronald , José Fazzio Feres Reinaldo , Coimbra Marigo Thaís , Maria Penteado-Dias Angélica
Published: July 2022

Two new opilioacarid species from Northeast and Southeast of Brazil are described. Neocarus setilatus sp. nov. from a gallery forest in Piauí and Neocarus marambaia sp. nov. from a restinga area in Rio de Janeiro. Both species have the similar starshaped integument character and smaller, broader setae. We discuss the importance of these characters with systematic value and present an updated taxonomic key for the genus Neocarus.
New species and records of ptyctimous mites (Acarina, Oribatida) from the Neotropical region
Authors: Niedbala Wojciech , Ermilov Sergey G.
Published: July 2022

The present study is based on ptyctimous mite materials collected from Brazil, Chile, Colombia, Dominicana, Peru, and Venezuela. A list of identified taxa, including 26 species from nine genera and five families is provided. Two new species—Phthiracarus alienus sp. nov. (Phthiracaridae) and Austrophthiracarus paradiazae sp. nov. (Steganacaridae)—are described. Morphological additions to description of Indotritia bellingeri are presented.
Massively parallel pooled screening reveals genomic determinants of nanoparticle delivery
Authors: Boehnke Natalie , Straehla Joelle P. , Safford Hannah C. , Kocak Mustafa , Rees Matthew G. , Ronan Melissa , Rosenberg Danny , Adelmann Charles H. , Chivukula Raghu R. , Nabar Namita , Berger Adam G. , Lamson Nicholas G. , Cheah Jaime H. , Li Hojun , Roth Jennifer A. , Koehler Angela N. , Hammond Paula T.
Published: July 2022

To accelerate the translation of cancer nanomedicine, we used an integrated genomic approach to improve our understanding of the cellular processes that govern nanoparticle trafficking. We developed a massively parallel screen that leverages barcoded, pooled cancer cell lines annotated with multiomic data to investigate cell association patterns across a nanoparticle library spanning a range of formulations with clinical potential. We identified both materials properties and cell-intrinsic features that mediate nanoparticle-cell association. Using machine learning algorithms, we constructed genomic nanoparticle trafficking networks and identified nanoparticle-specific biomarkers. We validated one such biomarker: gene expression ofSLC46A3, which inversely predicts lipid-based nanoparticle uptake in vitro and in vivo. Our work establishes the power of integrated screens for nanoparticle delivery and enables the identification and utilization of biomarkers to rationally design nanoformulations.