bluuebunny/crossref_metadata_2025 huggingface dataset:
Attenuation of immobilization stress-induced hypertension by temperature-controllable warm needle acupuncture in rats and the peripheral neural mechanisms
Authors: Bang Se Kyun , Chang Suchan , Seo Su Yeon , Kang Suk-Yun , Cho Seong Jin , Choi Kwang-Ho , Juping Xing , Kim Hee Young , Ryu Yeonhee
Published: June 2023

Introduction

We and others have shown that electrical stimulation of the PC-6 acupoint over the wrist relieves hypertension by stimulating afferent sensory nerve fibers and activating the central endogenous opioid system. Warm needle acupuncture has long been utilized to treat various diseases in clinics.

Methods

Here, we developed a temperature-controllable warm needle acupuncture instrument (WAI) and investigated the peripheral mechanism underlying the effect of warm needle acupuncture at PC-6 on hypertension in a rat model of immobilization stress-induced hypertension.

Results

Stimulation with our newly developed WAI and traditional warm needle acupuncture attenuated hypertension development. Such effects were reproduced by capsaicin (a TRPV1 agonist) injection into PC-6 or WAI stimulation at 48°C. In contrast, PC-6 pretreatment with the TRPV1 antagonist capsazepine blocked the antihypertensive effect of WAI stimulation at PC-6. WAI stimulation at PC-6 increased the number of dorsal root ganglia double-stained with TRPV1 and CGRP. QX-314 and capsaicin perineural injection into the median nerve for chemical ablation of small afferent nerve fibers (C-fibers) prevented the antihypertensive effect of WAI stimulation at PC-6. Additionally, PC-6 pretreatment with RTX ablated the antihypertensive effect of WAI stimulation.

Conclusion

These findings suggest that warm needle acupuncture at PC-6 activates C-fiber of median nerve and the peripheral TRPV1 receptors to attenuate the development of immobilization stress-induced hypertension in rats.
Modulation of the thalamus by microburst vagus nerve stimulation: a feasibility study protocol
Authors: Verner Ryan , Szaflarski Jerzy P. , Allendorfer Jane B. , Vonck Kristl , Giannicola Gaia ,   Microburst Study Group
Published: June 2023

Vagus nerve stimulation (VNS) was the first device-based therapy for epilepsy, having launched in 1994 in Europe and 1997 in the United States. Since then, significant advances in the understanding of the mechanism of action of VNS and the central neurocircuitry that VNS modulates have impacted how the therapy is practically implemented. However, there has been little change to VNS stimulation parameters since the late 1990s. Short bursts of high frequency stimulation have been of increasing interest to other neuromodulation targets e.g., the spine, and these high frequency bursts elicit unique effects in the central nervous system, especially when applied to the vagus nerve. In the current study, we describe a protocol design that is aimed to assess the impact of high frequency bursts of stimulation, called “Microburst VNS”, in subjects with refractory focal and generalized epilepsies treated with this novel stimulation pattern in addition to standard anti-seizure medications. This protocol also employed an investigational, fMRI-guided titration protocol that permits personalized dosing of Microburst VNS among the treated population depending on the thalamic blood-oxygen-level-dependent signal. The study was registered on clinicaltrials.gov (NCT03446664). The first subject was enrolled in 2018 and the final results are expected in 2023.
Association between morphologic features of intracranial distal arteries and brain atrophy indexes in cerebral small vessel disease: a voxel-based morphometry study
Authors: Cheng Hongjiang , Teng Junfang , Jia Longbin , Xu Lina , Yang Fengbing , Li Huimin , Ling Chen , Liu Wei , Li Jinna , Li Yujuan , Guo Zixuan , Geng Xia , Guo Jiaying , Zhang Dandan
Published: June 2023

Background

Brain atrophy represents a final common pathway for pathological processes in patients with cerebral small vessel disease (CSVD) and is now recognized as a strong independent predictor of clinical status and progression. The mechanism underlying brain atrophy in patients with CSVD is not yet fully comprehended. This study aims to investigate the association of morphologic features of intracranial distal arteries (A2, M2, P2 and more distal) with different brain structures [gray matter volume (GMV), white matter volume (WMV), and cerebrospinal fluid volume (CSFV)]. Furthermore, we also examined whether a correlation existed between these cerebrovascular characteristics and GMV in different brain regions.

Method

A total of 39 participants were eventually enrolled. The morphologic features of intracranial distal arteries based on TOF-MRA were extracted and quantified using the intracranial artery feature extraction technique (iCafe). The brain 3D-T1 images were segmented into gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) using the “Segment” tool in CAT12 for the voxel-based morphometry (VBM) analysis. Univariable and multivariable linear regression models were used to investigate the relationship between these cerebrovascular features and different brain structures. Partial correlation analysis with a one-tailed method was used to evaluate the relationship between these cerebrovascular features and GMV in different brain regions.

Results

Our findings indicate that both distal artery length and density were positively correlated with GM fraction in CSVD patients, regardless of whether univariable or multivariable linear regression analyses were performed. In addition, distal artery length (β = −0.428, p = 0.007) and density (β = −0.337, p = 0.036) were also found to be negative associated with CSF fraction, although this relationship disappeared after adjusting for potential confounders. Additional adjustment for the effect of WMHs volume did not change these results. In subgroup anasysis, we found that participants in the highest distal artery length tertile had significantly higher GM fraction and lower CSF fraction level than participants in the lowest distal artery length tertile. In partial correlation analysis, we also found that these cerebrovascular characteristics associated with regional GMV, especially subcortical nuclear.

Conclusion

The morphologic features of intracranial distal arteries, including artery length, density and average tortuosity, measured from 3D-TOF MRA, are associated with generalized or focal atrophy indexes of CSVD.
Digital endpoints in clinical trials of Alzheimer’s disease and other neurodegenerative diseases: challenges and opportunities
Authors: Brem Anna-Katharine , Kuruppu Sajini , de Boer Casper , Muurling Marijn , Diaz-Ponce Ana , Gove Dianne , Curcic Jelena , Pilotto Andrea , Ng Wan-Fai , Cummins Nicholas , Malzbender Kristina , Nies Vera J. M. , Erdemli Gul , Graeber Johanna , Narayan Vaibhav A. , Rochester Lynn , Maetzler Walter , Aarsland Dag
Published: June 2023

Alzheimer’s disease (AD) and other neurodegenerative diseases such as Parkinson’s disease (PD) and Huntington’s disease (HD) are associated with progressive cognitive, motor, affective and consequently functional decline considerably affecting Activities of Daily Living (ADL) and quality of life. Standard assessments, such as questionnaires and interviews, cognitive testing, and mobility assessments, lack sensitivity, especially in early stages of neurodegenerative diseases and in the disease progression, and have therefore a limited utility as outcome measurements in clinical trials. Major advances in the last decade in digital technologies have opened a window of opportunity to introduce digital endpoints into clinical trials that can reform the assessment and tracking of neurodegenerative symptoms. The Innovative Health Initiative (IMI)-funded projects RADAR-AD (Remote assessment of disease and relapse—Alzheimer’s disease), IDEA-FAST (Identifying digital endpoints to assess fatigue, sleep and ADL in neurodegenerative disorders and immune-mediated inflammatory diseases) and Mobilise-D (Connecting digital mobility assessment to clinical outcomes for regulatory and clinical endorsement) aim to identify digital endpoints relevant for neurodegenerative diseases that provide reliable, objective, and sensitive evaluation of disability and health-related quality of life. In this article, we will draw from the findings and experiences of the different IMI projects in discussing (1) the value of remote technologies to assess neurodegenerative diseases; (2) feasibility, acceptability and usability of digital assessments; (3) challenges related to the use of digital tools; (4) public involvement and the implementation of patient advisory boards; (5) regulatory learnings; and (6) the significance of inter-project exchange and data- and algorithm-sharing.
Opening the black box of traumatic brain injury: a holistic approach combining human 3D neural tissue and an in vitro traumatic brain injury induction device
Authors: Loussert-Fonta Céline , Stoppini Luc , Neuenschwander Yoan , Righini Ophélie , Prim Denis , Schmidt Cédric , Heuschkel Marc O. , Gomez Baisac Loris , Jovic´ Milica , Pfeifer Marc E. , Extermann Jérôme , Roux Adrien
Published: June 2023

Traumatic brain injury (TBI) is caused by a wide range of physical events and can induce an even larger spectrum of short- to long-term pathophysiologies. Neuroscientists have relied on animal models to understand the relationship between mechanical damages and functional alterations of neural cells. These in vivo and animal-based in vitro models represent important approaches to mimic traumas on whole brains or organized brain structures but are not fully representative of pathologies occurring after traumas on human brain parenchyma. To overcome these limitations and to establish a more accurate and comprehensive model of human TBI, we engineered an in vitro platform to induce injuries via the controlled projection of a small drop of liquid onto a 3D neural tissue engineered from human iPS cells. With this platform, biological mechanisms involved in neural cellular injury are recorded through electrophysiology measurements, quantification of biomarkers released, and two imaging methods [confocal laser scanning microscope (CLSM) and optical projection tomography (OPT)]. The results showed drastic changes in tissue electrophysiological activities and significant releases of glial and neuronal biomarkers. Tissue imaging allowed us to reconstruct the injured area spatially in 3D after staining it with specific nuclear dyes and to determine TBI resulting in cell death. In future experiments, we seek to monitor the effects of TBI-induced injuries over a prolonged time and at a higher temporal resolution to better understand the subtleties of the biomarker release kinetics and the cell recovery phases.
Plant to animal protein ratio in the diet: nutrient adequacy, long-term health and environmental pressure
Authors: Fouillet Hélène , Dussiot Alison , Perraud Elie , Wang Juhui , Huneau Jean-François , Kesse-Guyot Emmanuelle , Mariotti François
Published: June 2023

Background

Animal and plant protein sources have contrasting relationships with nutrient adequacy and long-term health, and their adequate ratio is highly debated.

Objective

We aimed to explore how the percentage of plant protein in the diet (%PP) relates to nutrient adequacy and long-term health but also to environmental pressures, to determine the adequate and potentially optimal %PP values.

Methods

Observed diets were extracted from the dietary intakes of French adults (INCA3, n = 1,125). Using reference values for nutrients and disease burden risks for foods, we modeled diets with graded %PP values that simultaneously ensure nutrient adequacy, minimize long-term health risks and preserve at best dietary habits. This multi-criteria diet optimization was conducted in a hierarchical manner, giving priority to long-term health over diet proximity, under the constraints of ensuring nutrient adequacy and food cultural acceptability. We explored the tensions between objectives and identified the most critical nutrients and influential constraints by sensitivity analysis. Finally, environmental pressures related to the modeled diets were estimated using the AGRIBALYSE database.

Results

We find that nutrient-adequate diets must fall within the ~15–80% %PP range, a slightly wider range being nevertheless identifiable by waiving the food acceptability constraints. Fully healthy diets, also achieving the minimum-risk exposure levels for both unhealthy and healthy foods, must fall within the 25–70% %PP range. All of these healthy diets were very distant from current typical diet. Those with higher %PP had lower environmental impacts, notably on climate change and land use, while being as far from current diet.

Conclusion

There is no single optimal %PP value when considering only nutrition and health, but high %PP diets are more sustainable. For %PP > 80%, nutrient fortification/supplementation and/or new foods are required.
Prevalence and socioeconomic determinants of food insecurity among Venezuelan migrant and refugee urban households in Peru
Authors: Hernández-Vásquez Akram , Vargas-Fernández Rodrigo , Visconti-Lopez Fabriccio J. , Aparco Juan Pablo
Published: June 2023

Introduction

Food insecurity (FI) is a public health problem affecting many regions of the world. In Venezuela, the political, social and economic situation experienced since 2010 has caused a mass migration of its population to other countries, including Peru, which, in turn, may have limited access to and availability of food leading to a high nutritional burden in this population. The objective of this study was to determine the prevalence and analyze the determinants of FI in the households of Venezuelan immigrants in Peru.

Methods

A cross-sectional study was conducted using the “Encuesta Dirigida a la Población Venezolana que Reside en el País” (ENPOVE 2022). The dependent variable was moderate–severe FI (yes/no), which was constructed from an eight-item Food Insecurity Experience Scale (FIES) to measure FI at the household level. Poisson log generalized linear regression models were fitted to assess the association between the independent variables and FI. In addition, the reliability of the FIES as a tool for measuring food insecurity in the target population was determined.

Results

A total of 3,491 households with Venezuelan migrants and refugees were included in the analysis. We found that 39.0% of Venezuelan immigrant households in Peru experienced moderate–severe FI. The determinants of FI included socio-demographic characteristics of the household head, and economic and geographical characteristics of the household. Regarding the FIES, we found that the inclusion of 7 of the 8 items had adequate internal consistency and its items assessed the same latent range.

Discussion

This study highlights the need to identify determinants associated with FI to design strategies that mitigate the consequences of health crises and strengthen regional food systems, making them more sustainable. Although several studies have evaluated the prevalence of FI in Venezuelan migrant populations in other countries, this study is the first to evaluate the determinants of FI in Venezuelan immigrant households in Peru.
Lipid oxidation in foods and its implications on proteins
Authors: Geng Lianxin , Liu Kunlun , Zhang Huiyan
Published: June 2023

Lipids in foods are sensitive to various environmental conditions. Under light or high temperatures, free radicals could be formed due to lipid oxidation, leading to the formation of unstable food system. Proteins are sensitive to free radicals, which could cause protein oxidation and aggregation. Protein aggregation significantly affects protein physicochemical characteristics and biological functions, such as digestibility, foaming characteristics, and bioavailability, further reducing the edible and storage quality of food. This review provided an overview of lipid oxidation in foods; its implications on protein oxidation; and the assessment methods of lipid oxidation, protein oxidation, and protein aggregation. Protein functions before and after aggregation in foods were compared, and a discussion for future research on lipid or protein oxidation in foods was presented.
Integration analysis of single-cell and spatial transcriptomics reveal the cellular heterogeneity landscape in glioblastoma and establish a polygenic risk model
Authors: Liu Yaxuan , Wu Zhenyu , Feng Yueyuan , Gao Jiawei , Wang Bo , Lian Changlin , Diao Bo
Published: June 2023

Background

Glioblastoma (GBM) is adults’ most common and fatally malignant brain tumor. The heterogeneity is the leading cause of treatment failure. However, the relationship between cellular heterogeneity, tumor microenvironment, and GBM progression is still elusive.

Methods

Integrated analysis of single-cell RNA sequencing (scRNA-seq) and spatial transcriptome sequencing (stRNA-seq) of GBM were conducted to analyze the spatial tumor microenvironment. We investigated the subpopulation heterogeneity of malignant cells through gene set enrichment analyses, cell communications analyses, and pseudotime analyses. Significantly changed genes of the pseudotime analysis were screened to create a tumor progress-related gene risk score (TPRGRS) using Cox regression algorithms in the bulkRNA-sequencing(bulkRNA-seq) dataset. We combined the TPRGRS and clinical characteristics to predict the prognosis of patients with GBM. Furthermore, functional analysis was applied to uncover the underlying mechanisms of the TPRGRS.

Results

GBM cells were accurately charted to their spatial locations and uncovered their spatial colocalization. The malignant cells were divided into five clusters with transcriptional and functional heterogeneity, including unclassified malignant cells and astrocyte-like, mesenchymal-like, oligodendrocytes-progenitor-like, and neural-progenitor-like malignant cells. Cell-cell communications analysis in scRNA-seq and stRNA-seq identified ligand-receptor pairs of the CXCL, EGF, FGF, and MIF signaling pathways as bridges implying that tumor microenvironment may cause malignant cells’ transcriptomic adaptability and disease progression. Pseudotime analysis showed the differentiation trajectory of GBM cells from proneural to mesenchymal transition and identified genes or pathways that affect cell differentiation. TPRGRS could successfully divide patients with GBM in three datasets into high- and low-risk groups, which was proved to be a prognostic factor independent of routine clinicopathological characteristics. Functional analysis revealed the TPRGRS associated with growth factor binding, cytokine activity, signaling receptor activator activity functions, and oncogenic pathways. Further analysis revealed the association of the TPRGRS with gene mutations and immunity in GBM. Finally, the external datasets and qRT-PCR verified high expressions of the TPRGRS mRNAs in GBM cells.

Conclusion

Our study provides novel insights into heterogeneity in GBM based on scRNA-seq and stRNA-seq data. Moreover, our study proposed a malignant cell transition-based TPRGRS through integrated analysis of bulkRNA-seq and scRNA-seq data, combined with the routine clinicopathological evaluation of tumors, which may provide more personalized drug regimens for GBM patients.
Proteome-wide comparison of tertiary protein structures reveals molecular mimicry in Plasmodium-human interactions
Authors: Muthye Viraj , Wasmuth James D.
Published: June 2023

Introduction

Molecular mimicry is a strategy used by parasites to evade the host’s immune system and facilitate transmission to a new host. To date, high-throughput examples of molecular mimicry have been limited to comparing protein sequences. However, recent advances in the prediction of tertiary structural models, led by Deepmind’s AlphaFold, enable the comparison of thousands of proteins from parasites and their hosts at the structural level, allowing for the identification of more mimics. Here, we present the first proteome-level search for tertiary structure similarity between proteins from Plasmodium falciparum, a malaria-causing parasite, and humans.

Methods

We assembled a database of experimentally-characterized protein tertiary structures (from the Protein Data Bank) and AlphaFold-generated protein tertiary structures from P. falciparum, human, and 15 negative control species, i.e., species not infected by P. falciparum. We aligned human and control structures to the parasite structures using Foldseek.

Results

We identified molecular mimicry in three proteins that have been previously proposed as mediators of Plasmodium-human interactions. By extending this approach to all P. falciparum proteins, we identified an additional 41 potential mimics that are supported by additional experimental data.

Discussion

Our findings demonstrate a valuable application of AlphaFold-derived tertiary structural models, and we discuss key considerations for its effective use in other host-parasite systems.
Cell-free supernatant of probiotic bacteria exerted antibiofilm and antibacterial activities against Pseudomonas aeruginosa: A novel biotic therapy
Authors: Drumond Mariana Martins , Tapia-Costa Ana Paula , Neumann Elisabeth , Nunes Álvaro Cantini , Barbosa Jorge Wanderson , Kassuha Diego E. , Mancha-Agresti Pamela
Published: June 2023

Aim: This study aims to verify the antibacterial and antibiofilm action of cell-free spent medium (CFSM) from four lactic acid bacteria with potential probiotic characteristics (Lactiplantibacillus plantarum, Lactobacillus acidophilus, Lactobacillus johnsonii, and Lactobacillus delbrueckii) against two Pseudomonas aeruginosa strains.

Main methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the CFSM, antibacterial activity by analysing the formation of inhibition zones, and inhibition of planktonic cultures were determined. Whether an increase in the concentration of CFSM influenced the growth of pathogenic strains and the anti-adhesive activity of the CFSM in biofilm formation (crystal violet and MTT assays) were determined, which were all corroborated by using scanning electron microscopy.

Key findings: The relationship between the MIC and MBC values showed a bactericidal or bacteriostatic effect for all the cell-free spent media (CFSMs) tested for P. aeruginosa 9027™ and 27853™ strains. The CFSM supplemental doses of 18 or 22%, 20 or 22%, 46 or 48%, and 50 or 54% of L. acidophilus, L. delbrueckii, L. plantarum, and L. johnsonii, respectively, could completely inhibit the growth of both pathogen strains. The antibiofilm activity of the CFSM in three biofilm conditions (pre-coated, co-incubated, and preformed) demonstrated values ranging between 40% and 80% for biofilm inhibition, and similar results were observed for cell viability.

Significance: This work provides strong evidence that the postbiotic derived from different Lactobacilli could be practical as an adjuvant therapy for reducing the use of antibiotics, being a good candidate to overcome the growing challenge of hospital infections due to this pathogen.
Inspiratory muscle fatigue at the swimming tumble turns: its occurrence and effects on kinematic parameters of the turns
Authors: Moravec Tadeja , Lomax Mitch , Ušaj Anton , Kapus Jernej
Published: June 2023

Introduction:The present study had two objectives: 1) to investigate the effects of tumble turns on the development of inspiratory muscle fatigue (IMF) and compare this to whole swimming, and 2) to evaluate the effects of pre-induced IMF on the kinematic parameters of tumble turns. Fourteen young club-level swimmers (13 ± 2 years of ages) completed three swim trials.

Methods:The first trial was used to determine the 400-m front crawl swim time at maximal effort (400FC). The other two trials consisted of a series of 15 tumble turns at the 400FC pace. In one of the turn-only trials, IMF was pre-induced (TURNS-IMF), whereas in the other turn-only trial it was not (TURNS-C).

Results:Compared with baseline values, the values for maximal inspiratory mouth pressure (PImax) at the end of the swim were significantly lower at all trials. However, the magnitude of inspiratory muscle fatigue was less after TURNS-C (PImax decreased by 12%) than after 400FC (PImax decreased by 28%). The tumble turns were slower during 400FC than during TURNS-C and TURNS-IMF. In addition, compared to TURNS-C, turns in the TURNS-IMF were performed with higher rotation times and shorter apnea and swim-out times.

Discussion:The results of the present study suggest that tumble turns put a strain on the inspiratory muscles and directly contribute to the IMF observed during 400FC swimming. Furthermore, pre-induced IMF resulted in significantly shorter apneas and slower rotations during tumble turns. IMF therefore has the potential to negatively affect overall swimming performance, and strategies should be sought to reduce its effects.
Endophytic infection increases the belowground over-yielding effects of the host grass community mainly by increasing the complementary effects
Authors: Qu Yaobing , Qin Tianzi , Zhang Junzhen , Deng Yongkang , Yu Xinhe , Wei Xianqin , Zhao Nianxi , Gao Yubao , Ren Anzhi
Published: June 2023

Introduction

Increases in plant species diversity may increase the community diversity effect and produce community over-yielding. Epichloë endophytes, as symbiotic microorganisms, are also capable of regulating plant communities, but their effects on community diversity effects are often overlooked.

Methods

In this experiment, we investigated the effects of endophytes on the diversity effects of host plant community biomass by constructing artificial communities with 1-species monocultures and 2- and 4-species mixtures of endophyte-infected (E+) and endophyte-free (E-) Achnatherum sibiricum and three common plants in its native habitat, which were potted in live and sterilized soil.

Results and discussion

The results showed that endophyte infection significantly increased the belowground biomass and abundance of Cleistogenes squarrosa, marginally significantly increased the abundance of Stipa grandis and significantly increased the community diversity (evenness) of the 4-species mixtures. Endophyte infection also significantly increased the over-yielding effects on belowground biomass of the 4-species mixtures in the live soil, and the increase in diversity effects on belowground biomass was mainly due to the endophyte significantly increasing the complementary effects on belowground biomass. The effects of soil microorganisms on the diversity effects on belowground biomass of the 4-species mixtures were mainly derived from their influences on the complementary effects. The effects of endophytes and soil microorganisms on the diversity effects on belowground biomass of the 4-species communities were independent, and both contributed similarly to the complementary effects on belowground biomass. The finding that endophyte infection promotes belowground over-yielding in live soil at higher levels of species diversity suggests that endophytes may be one of the factors contributing to the positive relationship between species diversity and productivity and explains the stable co-existence of endophyte-infected Achnatherum sibiricum with a variety of plants in the Inner Mongolian grasslands.
Effects of physiological integration on nitrogen use efficiency of moso bamboo in homogeneous and heterogeneous environments
Authors: Zhao Jiancheng , Cai Chunju
Published: June 2023

Introduction

Moso bamboo is one of the important clonal plants with complex underground rhizome-root system. Ramets connected by rhizome can translocate and share nitrogen (N), which may affect the nitrogen use efficiency (NUE) of moso bamboo. The aims of this study were to investigate the mechanisms of N physiological integration and its relationship with NUE of moso bamboo.

Methods

A pot experiment was conducted to trace the movement of 15N between the connected ramets of moso bamboo in both homogeneous and heterogeneous N environments.

Results

Results showed that N translocation within clonal fragments of moso bamboo was detected in both homogeneous and heterogeneous environments. The intensity of physiological integration (IPI) was significantly lower in homogeneous environments than that in heterogeneous environments. 15N translocation between the connected ramtes of moso bamboo was determined by the source-sink relationship in heterogeneous environments, and the 15N allocation of the fertilized ramet was higher than that of the connected unfertilized ramet. The NUE of connected treatment was significantly higher than that of severed treatment, which suggested that physiological integration significantly improved the NUE of moso bamboo. In addition, the NUE of moso bamboo was significantly higher in heterogeneous environments than that in homogeneous environments. The contribution rate of physiological integration (CPI) on NUE in heterogeneous environments was significantly higher than that in homogenous environments.

Discussion

These results will provide theoretical basis for precision fertilization in moso bamboo forests.
Genome-wide analysis and expression profiling of glyoxalase gene families in oat (Avena sativa) indicate their responses to abiotic stress during seed germination
Authors: Sun Ming , Sun Shoujiang , Jia Zhicheng , Zhang Han , Ou Chengming , Ma Wen , Wang Juan , Li Manli , Mao Peisheng
Published: June 2023

Abiotic stresses have deleterious effects on seed germination and seedling establishment, leading to significant crop yield losses. Adverse environmental conditions can cause the accumulation of methylglyoxal (MG) within plant cells, which can negatively impact plant growth and development. The glyoxalase system, which consists of the glutathione (GSH)-dependent enzymes glyoxalase I (GLX1) and glyoxalase II (GLX2), as well as the GSH-independent glyoxalase III (GLX3 or DJ-1), plays a crucial role in detoxifying MG. However, genome-wide analysis of glyoxalase genes has not been performed for one of the agricultural important species, oat (Avena sativa). This study identified a total of 26 AsGLX1 genes, including 8 genes encoding Ni2+-dependent GLX1s and 2 genes encoding Zn2+-dependent GLX1s. Additionally, 14 AsGLX2 genes were identified, of which 3 genes encoded proteins with both lactamase B and hydroxyacylglutathione hydrolase C-terminal domains and potential catalytic activity, and 15 AsGLX3 genes encoding proteins containing double DJ-1 domains. The domain architecture of the three gene families strongly correlates with the clades observed in the phylogenetic trees. The AsGLX1, AsGLX2, and AsGLX3 genes were evenly distributed in the A, C, and D subgenomes, and gene duplication of AsGLX1 and AsGLX3 genes resulted from tandem duplications. Besides the core cis-elements, hormone responsive elements dominated the promoter regions of the glyoxalase genes, and stress responsive elements were also frequently observed. The subcellular localization of glyoxalases was predicted to be primarily in the cytoplasm, chloroplasts, and mitochondria, with a few presents in the nucleus, which is consistent with their tissue-specific expression. The highest expression levels were observed in leaves and seeds, indicating that these genes may play important roles in maintaining leaf function and ensuring seed vigor. Moreover, based on in silico predication and expression pattern analysis, AsGLX1-7A, AsGLX2-5D, AsDJ-1-5D, AsGLX1-3D2, and AsGLX1-2A were suggested as promising candidate genes for improving stress resistance or seed vigor in oat. Overall, the identification and analysis of the glyoxalase gene families in this study can provide new strategies for improving oat stress resistance and seed vigor.
Translation, cross-cultural adaptation and validation of the 10-item Weekly Calendar Planning Activity in Spanish-speaking ABI patients: a multicenter study
Authors: Salazar-Frías Daniel , Funes María Jesús , Merchán-Baeza Jose Antonio , Ricchetti Giorgia , Torralba-Muñoz Jose Maria , Rodríguez-Bailón María
Published: June 2023

We present the process of translation, adaptation, and validation in the Spanish context of the 10-item version of the Weekly Calendar Planning Activity (WCPA-10), a performance-based measure of cognitive instrumental activities of daily living (C-IADL). The study consisted of two phases: I) translation/cultural adaptation of the WCPA, conducted by professional bilingual translators, a panel of experts, and a pilot study, and II) validation in a sample of 42 acquired brain injury patients (ABI) and 42 healthy participants (HC). WCPA primary outcomes showed expected convergent/discriminant validity patterns with socio-demographical and clinical variables and cognitive processes identifying those WCPA outcomes that best predicted executive and memory deficits measured with a battery of traditional neuropsychological tests. In addition, performance on the WCPA was a significant predictor of everyday functioning over variables such as socio-demographics or global cognition when measured with traditional tests. External validity was established by the WCPA's ability to identify everyday cognitive deficits in ABI patients compared to HC, even in those with subtle cognitive impairment based on neuropsychological tests. The Spanish WCPA-10 seems an appropriate and sensitive assessment tool to identify cognitive-functional impairment in ABI-patients, even those with subtle cognitive impairment. The results also highlight the relevance of this kind of test, as they indicate a better prediction of patients' real-world functioning than traditional neuropsychological tests.
Enjoyment, boredom, and perceived effectiveness of learners in language MOOCs: the mediating effect of self-regulated learning
Authors: Luo Rong , Wang Yijin
Published: June 2023

Self-regulated learning in technology-supported environments has attracted much scholarly attention in recent years. With the rapid expansion of online education, students’ emotions have also been studied extensively in second language acquisition. However, few empirical studies have examined the interrelationship between students’ self-regulated learning and emotions in the emerging field of language MOOCs (LMOOCs). This study bridged this gap by exploring the relationship between foreign language enjoyment (FLE), boredom (FLB), self-regulated learning (SRL), and perceived effectiveness in LMOOC learning. Data were collected among 356 successful learners of a language MOOC in mainland China through a cross-sectional study. The results showed that LMOOC learners had a high level of enjoyment and a moderate level of boredom. A significantly positive relationship was noted between FLE and SRL while a negative relationship was found between FLB and SRL. SRL was confirmed to be the mediator between FLE, FLB, and PE, which partially mediated the effects of FLE on PE and fully mediated the effects of FLB on PE. Perceived effectiveness was predicted by all SRL strategies and time management significantly predicted perceived effectiveness. The results provided pedagogical implications for students to develop positive emotions and effective SRL strategies to achieve better learning outcomes in LMOOC learning.
Generalization of the modulatory effect of social interaction on personal space
Authors: Cui Shulei , Yang Tianshu , Liu Ning
Published: June 2023

Introduction

Personal space (PS) is a safe area around an individual’s body that affects spatial distance when socially interacting with others. Previous studies have shown that social interaction may modulate PS. However, these findings are often confounded by the effects of familiarization. Furthermore, whether the potential regulatory effects of social interaction on PS can be generalized from interacting confederates to strangers remains unclear.

Methods

To answer these questions, we enrolled 115 participants in a carefully designed experiment.

Results

We found that prosocial interaction in the form of a cooperative task effectively reduced PS, and this regulatory effect could be generalized from interacting confederates to non-interacting confederates.

Discussion

These findings deepen our understanding of PS regulation and may be aid in the diagnosis and rehabilitation of dysfunctional social behaviors.
Emotional relevance and prejudice: testing the differentiated effect of incidental disgust on prejudice towards ethnic minorities
Authors: Pascal Emilia , Holman Andrei Corneliu , Miluț Felicia Mihaela
Published: June 2023

Negative emotions such as disgust or anger influence the evaluation of minorities and amplify prejudice, stereotypes, and discrimination behaviors towards them. However, new discoveries suggest that these spillover effects might be more specific in the sense that the bias might occur only if the emotions are specific to the affect that is generally evoked by that particular minority, i.e. anger increases prejudice towards anger-relevant groups, and disgust towards disgust-relevant groups. Our study aimed to examine, the specificity of the spillover effects, namely the importance of emotion’s relevance to the prejudice towards out-groups. To test this hypothesis, we investigated the influence of incidental disgust on the evaluation of two minorities, one that is usually associated with disgust (the Roma minority) and one usually associated with anger (the Hungarian minority). We used a 2 × 2 between-subjects experimental design where we manipulated the emotion experienced by the participants (disgust versus neutral) and the target they evaluated (Romani or Hungarian minority). We tested the effects of these manipulations on three aspects of prejudice toward the target group: cognitive, affective, and behavioral. The results support the specificity of the spillover effect, by showing that incidental disgust increased prejudice only towards the disgust-relevant target, namely the Roma minority, and that the intensity of this emotion experienced by the participants mediates this effect. Moreover, incidental disgust increased not only the negative emotions associated with the Romani (i.e., the affective component) but also the negative cognitions associated with them and the desire to maintain an increased social distance (i.e., behavioral prejudice). These findings highlight the importance of emotions’ relevance in bias toward minorities and provide a starting point for future anti-discrimination interventions.
Enhancing Chinese students’ academic engagement: the effect of teacher support and teacher–student rapport
Authors: Pan Xiaoquan , Yao Yuanyuan
Published: June 2023

Academic engagement plays an undeniable role in students’ leaning outcome. Therefore, identifying the influential antecedents of promoting students’ academic engagement is extremely crucial. Despite previous empirical studies have delved into the part played by several student-related and teacher-related factors in triggering Chinese students’ academic engagement, the exploration on the roles of teacher support and teacher–student rapport is still scant. Thus, this study attempts to concentrate on the influence of teacher support and teacher–student rapport on undergraduate students’ academic engagement in China. Three scales of the questionnaire—one each for teacher’s support, student-teacher rapport, and the level of academic engagement—were completed by a total of 298 undergraduate students. Spearman Rho test was adopted to detect the correlations between the variables. Following that, multiple regression analysis was used to estimate the predictive power of the dependent variables. The result found that teacher support and teacher–student rapport exert a tremendous influence on boosting Chinese students’ academic engagement. The leading implications and future directions are also presented.
Exploring crossmodal correspondences for future research in human movement augmentation
Authors: Pinardi Mattia , Di Stefano Nicola , Di Pino Giovanni , Spence Charles
Published: June 2023

“Crossmodal correspondences” are the consistent mappings between perceptual dimensions or stimuli from different sensory domains, which have been widely observed in the general population and investigated by experimental psychologists in recent years. At the same time, the emerging field of human movement augmentation (i.e., the enhancement of an individual’s motor abilities by means of artificial devices) has been struggling with the question of how to relay supplementary information concerning the state of the artificial device and its interaction with the environment to the user, which may help the latter to control the device more effectively. To date, this challenge has not been explicitly addressed by capitalizing on our emerging knowledge concerning crossmodal correspondences, despite these being tightly related to multisensory integration. In this perspective paper, we introduce some of the latest research findings on the crossmodal correspondences and their potential role in human augmentation. We then consider three ways in which the former might impact the latter, and the feasibility of this process. First, crossmodal correspondences, given the documented effect on attentional processing, might facilitate the integration of device status information (e.g., concerning position) coming from different sensory modalities (e.g., haptic and visual), thus increasing their usefulness for motor control and embodiment. Second, by capitalizing on their widespread and seemingly spontaneous nature, crossmodal correspondences might be exploited to reduce the cognitive burden caused by additional sensory inputs and the time required for the human brain to adapt the representation of the body to the presence of the artificial device. Third, to accomplish the first two points, the benefits of crossmodal correspondences should be maintained even after sensory substitution, a strategy commonly used when implementing supplementary feedback.
Conceptual understanding in science learning and the role of four psychometric variables: a person-centered approach
Authors: Vaiopoulou Julie , Tsikalas Themistocles , Stamovlasis Dimitrios , Papageorgiou George
Published: June 2023

The present study investigated conceptual understanding in learning science in relation to four cognitive variables: logical thinking, field-dependence/field-independence, and divergent and convergent thinking. The participants were fifth- and sixth-grade elementary school pupils involved in different mental tasks, where they had to describe and interpret phenomena related to changes of matter. This brief report presents data from the students’ understanding of evaporation, and the method of analysis, a person-centered approach, is explicated. Latent class analysis (LCA) was applied to reveal distinct clusters of cases sharing similar patterns of responses. The use of LCA aligns with theoretical conjectures related to a stepwise conceptual change process, and the hypothetical steps correspond to the identified discrete latent classes (LCs). Subsequently, the LCs were associated with the four cognitive variables as covariates, thus providing empirical evidence for the role of the above-mentioned individual differences in children’s learning in sciences. Methodological issues and theoretical implications are discussed.
Examining interhemispheric processing and task demand in lexical decision-making: insights from lateralized visual field paradigm
Authors: Kim Sangyub , Nam Kichun
Published: June 2023

This study aimed to investigate the influence of task demand on the uni−/bi-hemispheric processing of lexical decision-making. Two types of nonwords were used in parafoveal and foveal lexical decision tasks (LDTs) to manipulate task demand. In Experiment 1, a visual half-field paradigm was utilized to evaluate the unihemispheric strategy in lexical decision, which revealed a significant response bias toward “word” at the RVF/LH in the pseudoword LDT in contrast with the nonword LDT, indicating the strategic use of orthographical legality in LH for word-pseudoword lexical decision. In Experiment 2, the study evaluated whether foveal lexical decision follows the orthographical legality strategy of LH in pseudoword LDT relative to the nonword LDT. The results showed a response bias toward “word” in the foveal pseudoword LDT in contrast with the foveal nonword LDT, suggesting the recruitment of LH in foveal pseudoword LDT. These findings support the left-dominant bihemispheric processing in foveal lexical decision and contribute to our understanding of the mechanisms underlying lexical decision-making.
Key insights from studies on the stability of personality disorders in different age groups
Authors: d’Huart Delfine , Seker Süheyla , Bürgin David , Birkhölzer Marc , Boonmann Cyril , Schmid Marc , Schmeck Klaus , Bach Bo
Published: June 2023

While for decades, temporal stability has been conceived as a defining feature of personality disorders (PDs), cumulative findings appear to question the stability of PDs and PD symptoms over time. However, stability itself is a complex notion and findings are highly heterogenous. Building upon a literature search from a systematic review and meta-analysis, this narrative review aims to capture key findings in order to provide critical implications, both for clinical practice and future research. Taken together, this narrative review revealed that unlike previous assumptions, stability estimates in adolescence are comparable to stability estimates in adulthood and PDs and PD symptoms are not that stable. The extent of stability itself depends yet on various conceptual, methodological, environmental, and genetic factors. While findings were thus highly heterogenous, they all seem to converge in a notable trend towards symptomatic remission, except for high-risk-samples. This challenges the current understanding of PDs in terms of disorders and symptoms and argues instead in favor of the AMPD and ICD-11 reintroducing the idea of self and interpersonal functioning as the core feature of PDs.