bluuebunny/crossref_metadata_2025 huggingface dataset:
Incidence and Predictors of Canine Parvovirus Diagnoses in Puppies Relocated for Adoption
Authors: DiGangi Brian A. , Craver Cathlin , Dolan Emily D.
Published: April 2021

Animal relocation programs seek to balance the animal population and resources between source and destination communities to promote positive outcomes, though little objective evidence has been reported on their physical and behavioral implications. The objective of the current report is to describe the incidence and predictors of canine parvovirus (CPV) diagnoses in 8- to 19-week-old puppies reported by destination shelters participating in a large scale, long-distance, structured animal relocation program. The incidence of post-transport CPV diagnoses in the study population of 4088 puppies was 2.3%. The number of pre-transport vaccinations, length of stay at the source shelter, and time between pre-transport vaccination and transport was not associated with the expected difference in count of post-transport CPV diagnoses (p > 0.05), and was lower in those 13–17 weeks of age (IRR = 0.08, 95% CI = 0.02–0.34, p = 0.001), 18–19 weeks of age (IRR = 0.11, 95% CI = 0.02–0.80, p = 0.029), transferred in to the source shelter (IRR = 0.31, 95% CI = 0.10–0.96, p = 0.043), and transported in the summer season (IRR = 0.07, 95% CI = 0.01–0.53, p = 0.010). When basic biosecurity practices and vaccination protocols were in place, post-transport CPV cases in puppies were few, suggesting that the timing of transport should take into account factors other than the number or timing of pre-transport vaccinations.
A Mother’s Story, Mitogenome Relationships in the Genus Rupicapra
Authors: Iacolina Laura , Buzan Elena , Safner Toni , Bašić Nino , Geric Urska , Tesija Toni , Lazar Peter , Arnal María Cruz , Chen Jianhai , Han Jianlin , Šprem Nikica
Published: April 2021

Although the two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently classified as least-concern by the IUCN (International Union for Conservation of Nature), inconsistencies on the subspecies classification reported in literature make it challenging to assess the conservation status of the single subspecies. Previous studies relying on mitochondrial genes, sometimes in combination with nuclear or Y-chromosome markers, reported the presence of clusters corresponding to the geographic distribution but highlighting ambiguities in the genus phylogeny. Here we report novel de novo assembled sequences of the mitochondrial genome from nine individuals, including previously unpublished R. r. balcanica and R. r. tatrica subspecies, and use them to untangle the genus phylogeny. Our results based on the full mitogenome inferred phylogeny confirm the previously reported genus subdivision in three clades and its monophyletic positioning within the Caprinae. Phylogeny and taxonomy of Rupicapra species thus remain controversial prompting for the inclusion of archeological remains to solve the controversy.
Evaluation of Genetic Diversity and Structure of Turkish Water Buffalo Population by Using 20 Microsatellite Markers
Authors: Ünal Emel Özkan , Işık Raziye , Şen Ayşe , Geyik Kuş Elif , Soysal Mehmet İhsan
Published: April 2021

The present study was aimed to investigate the genetic diversity among 17 Turkish water buffalo populations. A total of 837 individuals from 17 provincial populations were genotyped, using 20 microsatellites markers. The microsatellite markers analyzed were highly polymorphic with a mean number of alleles of (7.28) ranging from 6 (ILSTS005) to 17 (ETH003). The mean observed and expected heterozygosity values across all polymorphic loci in all studied buffalo populations were 0.61 and 0.70, respectively. Observed heterozygosity varied from 0.55 (Bursa (BUR)) to 0.70 (Muş (MUS)). It was lower than expected heterozygosity in most of the populations indicating a deviation from Hardy–Weinberg equilibrium. The overall value for the polymorphic information content of noted microsatellite loci was 0.655, indicating their suitability for genetic diversity analysis in buffalo. The mean FIS value was 0.091 and all loci were observed significantly deviated from Hardy–Weinberg Equilibrium (HWE), most likely based on non-random breeding. The 17 buffalo populations were genetically less diverse as indicated by a small mean FST value (0.032 ± 0.018). The analysis of molecular variance (AMOVA) analysis indicated that about 2% of the total genetic diversity was clarified by population distinctions and 88 percent corresponded to differences among individuals. The information produced by this study can be used to establish a base of national conservation and breeding strategy of water buffalo population in Turkey.
Can the Hole–Board Test Predict a Rat’s Exploratory Behavior in a Free-Exploration Test?
Authors: Pisula Wojciech , Modlinska Klaudia , Goncikowska Katarzyna , Chrzanowska Anna
Published: April 2021

This study focuses on the rat activity in a hole–board setting that we considered a type of exploratory behavior. The general hypothesis is based on the claim that a motivational mechanism is central to both the response to novelty in a highly familiarized environment and the activity in the hole–board apparatus. Our sample consisted of 80 experimentally naive Lister Hooded rats. All rats were tested in the hole–board apparatus. Twenty individuals with the highest hole-board scores and twenty subjects with the lowest hole–board scores subsequently underwent an established free-exploration test. In our study, the scores obtained in the hole–board test had little predictive value for the rats’ activity in the free-exploration test. Based on our previous experience in studying exploratory behavior in the free-exploration test and the data presented in this paper, we suggest that the hole–board test is not an appropriate tool for measuring exploratory behavior in laboratory rodents.
An Illustrative Analysis of Atypical Gas Production Profiles Obtained from In Vitro Digestibility Studies Using Fecal Inoculum
Authors: Dhanoa Mewa S. , López Secundino , Powell Christopher D. , Sanderson Ruth , Ellis Jennifer L. , Murray Jo-Anne M. D. , Garber Anna , Williams Barbara A. , France James
Published: April 2021

Gas production profiles typically show a monotonically increasing monophasic pattern. However, atypical gas production profiles exist whereby at least two consecutive phases of gas production or additional extraneous features that distort the typical profile are present. Such profiles are more likely to occur with the use of a fecal inoculum and are much less well described. The presence of multiple phases or non-descript extraneous features makes it difficult to apply directly recommended modeling approaches such as standard response functions or classical growth functions. To overcome such difficulties, extensions of the Mitscherlich equation and a numerical modeling option also based on the Mitscherlich are explored. The numerical modeling option uses an estimate of relative rate obtained from the smoothed data profile and an estimate of maximum gas produced together with any lag time information drawn from the raw data to construct a simple Mitscherlich equation. In summary, this article illustrates the analysis of atypical gas production profiles obtained using a fecal inoculum and explores the methodology of numerical modeling to reconstruct equivalent typical growth-like trends.
All Shades of Shrimp: Preferences of Colour Morphs of a Freshwater Shrimp Neocaridina davidi (Decapoda, Atyidae) for Substrata of Different Colouration
Authors: Plichta Zuzanna , Kobak Jarosław , Maciaszek Rafał , Kakareko Tomasz
Published: April 2021

An ornamental freshwater shrimp, Neocaridina davidi, is popular as an aquarium hobby and, therefore, a potentially invasive species. There is a growing need for proper management of this species to determine not only their optimum breeding conditions, but also their ability to colonise novel environments. We tested habitat preferences of colour morphs (brown, red, white) of N. davidi for substratum colour (black, white, grey shades, red) and fine or coarse chess-board patterns to recognise their suitable captivity conditions and predict their distribution after potential release into nature. We conducted laboratory choice experiments (n = 8) with three individuals of the same morph exposed for two hours to a range of backgrounds. Shrimp preferred dark backgrounds over light ones irrespective of their own colouration and its match with the background colour. Moreover, the brown and red morphs, in contrast to the white morph, preferred the coarse background pattern over the finer pattern. This suggests that the presence of dark, uniform substrata (e.g., rocks, macrophytes) will favour N. davidi. Nevertheless, the polymorphism of the species has little effect on its total niche breadth, and thus its invasive potential.
Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, Escherichia coli Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania
Authors: Kimera Zuhura I. , Mgaya Fauster X. , Misinzo Gerald , Mshana Stephen E. , Moremi Nyambura , Matee Mecky I. N.
Published: April 2021

We determined the phenotypic profile of multidrug-resistant (MDR) Escherichia coli isolated from 698 samples (390 and 308 from poultry and domestic pigs, respectively). In total, 562 Enterobacteria were isolated. About 80.5% of the isolates were E. coli. Occurrence of E. coli was significantly higher among domestic pigs (73.1%) than in poultry (60.5%) (p = 0.000). In both poultry and domestic pigs, E. coli isolates were highly resistant to tetracycline (63.5%), nalidixic acid (53.7%), ampicillin (52.3%), and trimethoprim/sulfamethoxazole (50.9%). About 51.6%, 65.3%, and 53.7% of E. coli were MDR, extended-spectrum beta lactamase-producing enterobacteriaceae (ESBL-PE), and quinolone-resistant, respectively. A total of 68% of the extended-spectrum beta lactamase (ESBL) producers were also resistant to quinolones. For all tested antibiotics, resistance was significantly higher in ESBL-producing and quinolone-resistant isolates than the non-ESBL producers and non-quinolone-resistant E. coli. Eight isolates were resistant to eight classes of antimicrobials. We compared phenotypic with genotypic results of 20 MDR E. coli isolates, ESBL producers, and quinolone-resistant strains and found 80% harbored blaCTX-M, 15% aac(6)-lb-cr, 10% qnrB, and 5% qepA. None harbored TEM, SHV, qnrA, qnrS, qnrC, or qnrD. The observed pattern and level of resistance render this portfolio of antibiotics ineffective for their intended use.
Overview and Evaluation of Existing Guidelines for Rational Antimicrobial Use in Small-Animal Veterinary Practice in Europe
Authors: Allerton Fergus , Prior Cameron , Bagcigil Arzu , Broens Els , Callens Bénédicte , Damborg Peter , Dewulf Jeroen , Filippitzi Maria-Eleni , Carmo Luís , Gómez-Raja Jonathan , Harpaz Erez , Mateus Ana , Nolff Mirja , Phythian Clare , Timofte Dorina , Zendri Flavia , Jessen Lisbeth
Published: April 2021

Antimicrobial stewardship guidelines (ASGs) represent an important tool to help veterinarians optimize their antimicrobial use with the objective of decreasing antimicrobial resistance. The aim of this study was to map and qualitatively assess the ASGs for antimicrobial use in cats and dogs in Europe. Country representatives of the European Network for Optimization of Veterinary Antimicrobial Treatment (ENOVAT) were asked to identify ASGs published in their countries. All collated ASGs updated since January 2010 containing recommendations on antimicrobial therapy for at least three conditions affecting different organ systems in cats and dogs underwent detailed review including AGREE II analysis. Out of forty countries investigated, fifteen ASGs from eleven countries met the inclusion criteria. Several critical principles of antimicrobial use were identified, providing a framework that should assist development of stewardship guidance. The AGREE II analysis highlighted several methodological limitations of the currently available ASGs. This study sheds light on the lack of national ASGs for dogs and cats in multiple European countries and should encourage national bodies to prioritize guideline development in small animals. A greater awareness of the need to use a structured approach to guideline development could improve the quality of ASGs in the future.
The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL Oxidation
Authors: Chiang Shu-Hua , Yang Kia-Min , Sheu Shiann-Cherng , Chen Chih-Wei
Published: April 2021

In this study, the inhibition of DNA oxidative damage and low-density lipoprotein (LDL) oxidation of royal jelly protein (RJP) hydrolysates obtained from two commercial proteases were investigated. The results showed that the inhibition of DNA oxidative damage induced by the Fenton reaction, RJP, RJPs hydrolyzed by alcalase (RJP-A), RJPs hydrolyzed by flavourzyme (RPJ-F) and RJP two-stage hydrolysates (RPJ-AF) all had the effect of inhibiting deoxyribose oxidative damage. The inhibition effect of RJP, RJP-A, RJP-F and RJP-AF (1.0 mg/mL) were 47.06%, 33.70%, 24.19% and 43.09%, respectively. In addition, studies have also found that both RJP and RJP hydrolysates can reduce the production of 8-OH-2′-dG and the order of its inhibitory ability is RJP-AF ≒ RJP-A > RJP-F > RJP. The inhibition of DNA damage induced by bleomycin-Fe3+/ascorbic acid (Asc) with the addition of RJP, RJP-A, RPJ-F and RPJ-AF were 17.16%, 30.88%, 25.00% and 37.25%, respectively. The results of LDL oxidation inhibition showed that RJP-AF (1 mg/mL) not only had the most effective inhibitory Cu2+-induced LDL oxidation to produce a thiobarbituric acid reactive substance (TBARS) but also extended the lag time of conjugated diene formation to 300 min, which was 3.3 times that of the control group.
Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco
Authors: Mansinhos Inês , Gonçalves Sandra , Rodríguez-Solana Raquel , Ordóñez-Díaz José Luis , Moreno-Rojas José Manuel , Romano Anabela
Published: April 2021

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with conventional solvents. Ultrasound-assisted extraction (UAE) for 60 min proved to be the best extraction condition, and proline:lactic acid (1:1) and choline chloride:urea (1:2) extracts showed the highest total phenolic contents (56.00 ± 0.77 mgGAE/gdw) and antioxidant activity [64.35 ± 1.74 mgTE/gdw and 72.13 ± 0.97 mgTE/gdw in 2.2-diphenyl-1-picrylhydrazyl (DPPH) and 2.2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, respectively]. These extracts also exhibited enzymes inhibitory capacity particularly against Tyr and AChE. Even so, organic acid-based NADES showed to be the best extractants producing extracts with considerable ability to inhibit enzymes. Twenty-four phenolic compounds were identified by HPLC-HRMS, being rosmarinic acid, ferulic acid and salvianolic acid B the major compounds. The results confirmed that the combination of UAE and NADES provide an excellent alternative to organic solvents for sustainable and green extraction, and have huge potential for use in industrial applications involving the extraction of bioactive compounds from plants.
Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage
Authors: Karunarathne Wisurumuni Arachchilage Hasitha Maduranga , Molagoda Ilandarage Menu Neelaka , Lee Kyoung Tae , Choi Yung Hyun , Yu Sang-Mi , Kang Chang-Hee , Kim Gi-Young
Published: April 2021

Anthocyanin-enriched polyphenols from the flower petals of H. syriacus L. (Malvaceae, AHs) possess anti-septic shock, anti-oxidant, and anti-melanogenic properties. However, whether AHs positively or negatively regulate ultraviolet B (UVB)-mediated photoaging and photodamage remains unclear. This study aims to investigate the protective effect of AHs against UVB-induced damage. We examined the photoprotective effects of AHs on UVB-induced apoptosis, endoplasmic reticulum (ER) stress, and mitochondrial reactive oxygen species (mtROS). AHs prevented UVB irradiation-induced apoptosis of HaCaT keratinocytes by inhibiting caspase activation and ROS production. Moreover, AHs restored the survival rate and the hatchability of UVB-irradiated zebrafish larvae without any abnormalities. Furthermore, AHs inhibited UVB-induced ER stress, resulting in a decrease in mtROS production via the stabilization of the mitochondrial membrane potential. Our results indicate that AHs inhibit UVB-induced apoptosis by downregulating total cytosolic ROof cytosolic CaS and ER-mediated mitoROS production in both HaCaT keratinocytes and zebrafish larvae. These findings provide evidence for the applications of AHs to protect skin from UVB-induced photodamage.
High-Resolution Pixel LED Headlamps: Functional Requirement Analysis and Research Progress
Authors: Wang Liping , Ma Jianshe , Su Ping , Huang Jianwei
Published: April 2021

High-resolution pixel LED headlamps are lighting devices that can produce high-resolution light distribution to adapt to road and traffic conditions, intelligently illuminate traffic areas, and assist drivers. Due to the complexity of roads and traffic conditions, the functional diversity of high-resolution pixel LEDs headlamps and traffic safety has come into question and is the subject of in-depth research conducted by car manufacturers and regulators. We summarize the current possible functions of high-resolution pixel LED headlamps and analyze ways in which they could be improved. This paper also discusses the prospect of new technologies in the future.
Short-Time/-Angle Spectral Analysis for Vibration Monitoring of Bearing Failures under Variable Speed
Authors: Sierra-Alonso Edgar F. , Caicedo-Acosta Julian , Orozco Gutiérrez Álvaro Ángel , Quintero Héctor F. , Castellanos-Dominguez German
Published: April 2021

Vibration-condition monitoring aims to detect bearing damages of rotating machinery’s incipient failures mainly through time–frequency methods because of their efficient analysis of nonstationary signals. However, by having failures with impulse behavior, short-term events have a tendency to be diluted under variable-speed conditions, while information on frequency changes tends to be lost. Here, we introduce an approach to highlighting bearing impulsive failures by measuring short-term spectral components to deal with variable-speed vibrations. The short-term estimator employs two sliding windows: a small one that measures the instantaneous amplitude level and tracks impulsive components and a large interval that evaluates the average background amplitude. Aiming to characterize cyclo-non-stationary processes with impulsive behavior, the emphasizing high-order-based estimator based on the principle of spectral entropy is introduced. For evaluation, both visual inspection and classifier performance are assessed, contrasting the spectral-entropy estimator with the widely used spectral-kurtosis approach for dealing with impulsive signals. The validation of short-time/-angle spectral analysis performed on three datasets at variable speed showed that the proposed spectral-entropy estimator is a promising indicator for emphasizing bearing failures with impulse behavior.
Estimation of Mode I Fracture of U-Notched Polycarbonate Specimens Using the Equivalent Material Concept and Strain Energy Density
Authors: Albinmousa Jafar , AlSadah Jihad , Hawwa Muhammad A. , Al-Qahtani Hussain M.
Published: April 2021

Polycarbonate (PC) has a wide range of applications in the electronic, transportation, and biomedical industries. In addition, investigation on the applicability to use PC in superstrate photovoltaic modules is ongoing research. In this paper, PC is envisioned to be used as a material for structural components in renewable energy systems. Usually, structural components have geometrical irregularities, i.e., notches, and are subjected to severe mechanical loading. Therefore, the structural integrity of these components shall consider fracture analysis on notched specimens. In this paper, rectangular PC specimens were machined with straight U-notches having different radii and depths. Eight different notch radii with a depth of 6.0 mm were tested. In addition, three notch depths with a radius of 3.5 mm were considered. Quasi-static fracture tests were performed under displacement-controlled loading with a speed of 5 mm/min. Digital image correlation technique was used to capture the strain fields for un-notched and notched specimens. It was assumed that fracture occurs at the onset of necking. The equivalent material concept (EMC) along with the strain energy density criterion (SED) were employed to estimate the fracture load. The EMC-SED combination is shown to be an effective and practical tool for estimating the fracture load of U-notched PC specimens.
Choice of Screws for Fixation of Mandibular Condyle Fractures Guided by Anthropometric Data
Authors: Bielecki-Kowalski Bartosz , Kozakiewicz Marcin
Published: April 2021

Open reduction and internal fixation (ORIF) is becoming increasingly common in treatment of the condylar process, including mandible head fractures. This approach significantly improves the results in terms of anatomical reduction of bone fragments, and shortens the treatment time, allowing for early functional recovery. The success of ORIF is largely determined by the stability of the osteosynthesis. The stabilization effect depends on the screw type and length of the plate used, in addition to the diameter and length of the screws used. The aim of this study was to determine the largest possible screw length that can be used in ORIF of the mandibular condyle considering the variable bone thickness. A total of 500 condyles were examined using computer tomography (CT)-based 3D models in Caucasians. For all models, three measurements were made in the frontal projection in places typical for the stabilization of osteosynthesis plates in the fractures of the condylar process: the base, the top, and the sigmoid notch. In addition, one measurement of the mandible head was made in the place of the greatest width. The results showed that 8 mm screws should be used in the region of the condylar base as the longest anatomically justified screw, whereas in the area of a sigmoid notch only 1.5–2 mm screws should be used. Measurements in the area of the neck top revealed statistically significant differences in the measurements between the sex of patients, with average differences below 1 mm (p < 0.05). In this area, the maximal length of the screw was found to be 10 mm. In mandibular head fractures, the use of long screws is extremely important due to the desired effect of fragment compression. Statistically significant differences were found in the measurement results between women and men. The maximal screw length for bicortical fixation was found to be 22 mm in men and 20 mm in women. In post-traumatic patients, the ability to obtain a clear measurement is often limited by a deformed anatomy. Taking into account the fact that the fracture stability is influenced by both the plate length and the length of the fixation screws, an assessment of the standard measurement values in a cohort group will improve the quality of the surgical fixations of the fractures.
Tunnel Failure Evolution and System Reliability Analysis Based on the β-unzipping Method
Authors: Yang Tianjiao , Wang Shuhong , Liu Huan
Published: April 2021

The structural failure of a tunnel is a process that evolves from local damage to overall destruction. The system reliability analysis method can be useful for analyzing the evolutionary law of local structural failure. In many complex stress environments, the structural performance function may be very complicated or even impossible to solve. This paper establishes a response surface function to represent the implicit tunnel performance function. The reliability of the shear capacity of a tunnel is considered. The critical parts and critical failure paths of the tunnel system are determined using the β-unzipping method. Failure evolution is analyzed to obtain the failure process of the tunnel system. Different failure modes are shown under different cases. Based on the partial failure probability and impact on the tunnel system, the risk levels of the critical parts at each stage of the failure history are evaluated. Therefore, the tunnel failure tree model obtained by combining the response surface method and β-unzipping method plays an important role in tunnel reliability evolution, and can evaluate tunnel safety comprehensively.
Towards Improving Transparency of Count Data Regression Models for Health Impacts of Air Pollution
Authors: Joseph John F. , Furl Chad , Sharif Hatim O. , Sunil Thankam , Macias Charles G.
Published: April 2021

In studies on the health impacts of air pollution, regression analysis continues to advance far beyond classical linear regression, which many scientists may have become familiar with in an introductory statistics course. With each new level of complexity, regression analysis may become less transparent, even to the analyst working with the data. This may be especially true in count data regression models, where the response variable (typically given the symbol y) is count data (i.e., takes on values of 0, 1, 2, …). In such models, the normal distribution (the familiar bell-shaped curve) for the residuals (i.e., the differences between the observed values and the values predicted by the regression model) no longer applies. Unless care is taken to correctly specify just how those residuals are distributed, the tendency to accept untrue hypotheses may be greatly increased. The aim of this paper is to present a simple histogram of predicted and observed count values (POCH), which, while rarely found in the environmental literature but presented in authoritative statistical texts, can dramatically reduce the risk of accepting untrue hypotheses. POCH can also increase the transparency of count data regression models to analysts themselves and to the scientific community in general.
Energy Characteristics of Full Tubular Pump Device with Different Backflow Clearances Based on Entropy Production
Authors: Meng Fan , Li Yanjun , Pei Ji
Published: April 2021

In this study, the entropy theory was used as the evaluation standard of energy dissipation, and the effect of backflow clearance (the gap between motor rotor and motor shell) on energy characteristics of a full tubular pump was investigated by 3D unsteady flow simulation. The calculated results validated through testing shows that backflow clearance produces additional head loss and the rotation of the motor rotor requires more shaft power. The additional energy losses lead to a significant decline in the efficiency of tubular pump device. Under design conditions, the total dissipation of backflow clearance, rear guide vane, and outlet passage decreases with the increase of clearance radius, but that of the impeller decreases first and then rises with the increase of clearance radius. In addition, the increase of the clearance radius leads to disorderly flow pattern in the impeller. The total dissipation rate on the impeller suction side and near the impeller inlet increases with the increase of backflow clearance radius, but that on the impeller suction side decreases with the increase of backflow clearance radius. The total dissipation rate of the suction side of the guide vane and the wall of the outlet passage decreases with the increase of backflow clearance radius. This work can provide an intuitive analysis of the energy dissipation caused by backflow clearance and reference for engineering applications of full tubular pump.
Several Cases for the Validation of Turbulence Models Implementation
Authors: Polewski Michael D. , Cizmas Paul G. A.
Published: April 2021

This paper presents several test cases that were used to validate the implementation of two turbulence models in the UNS3D code, an in-house code. The two turbulence models used were the Shear Stress Transport model and the Spalart–Allmaras model. These turbulence models were explored using the numerical results generated by three computational fluid dynamics codes: NASA’s FUN3D and CFL3D, and UNS3D. Four cases were considered: a flat plate case, an airfoil near-wake, a backward-facing step, and a turbine cascade known as the Eleventh Standard Configuration. The numerical results were compared among themselves and against experimental data.
The Modified Void Nucleation and Growth Model (MNAG) for Damage Evolution in BCC Ta
Authors: Chen Jie , Luscher Darby J. , Fensin Saryu J.
Published: April 2021

A void coalescence term was proposed as an addition to the original void nucleation and growth (NAG) model to accurately describe void evolution under dynamic loading. The new model, termed as modified void nucleation and growth model (MNAG model), incorporated analytic equations to explicitly account for the evolution of the void number density and the void volume fraction (damage) during void nucleation, growth, as well as the coalescence stage. The parameters in the MNAG model were fitted to molecular dynamics (MD) shock data for single-crystal and nanocrystalline Ta, and the corresponding nucleation, growth, and coalescence rates were extracted. The results suggested that void nucleation, growth, and coalescence rates were dependent on the orientation as well as grain size. Compared to other models, such as NAG, Cocks–Ashby, Tepla, and Tonks, which were only able to reproduce early or later stage damage evolution, the MNAG model was able to reproduce all stages associated with nucleation, growth, and coalescence. The MNAG model could provide the basis for hydrodynamic simulations to improve the fidelity of the damage nucleation and evolution in 3-D microstructures.
Predictive Maintenance: A Novel Framework for a Data-Driven, Semi-Supervised, and Partially Online Prognostic Health Management Application in Industries
Authors: Calabrese Francesca , Regattieri Alberto , Bortolini Marco , Gamberi Mauro , Pilati Francesco
Published: April 2021

Prognostic Health Management (PHM) is a predictive maintenance strategy, which is based on Condition Monitoring (CM) data and aims to predict the future states of machinery. The existing literature reports the PHM at two levels: methodological and applicative. From the methodological point of view, there are many publications and standards of a PHM system design. From the applicative point of view, many papers address the improvement of techniques adopted for realizing PHM tasks without covering the whole process. In these cases, most applications rely on a large amount of historical data to train models for diagnostic and prognostic purposes. Industries, very often, are not able to obtain these data. Thus, the most adopted approaches, based on batch and off-line analysis, cannot be adopted. In this paper, we present a novel framework and architecture that support the initial application of PHM from the machinery producers’ perspective. The proposed framework is based on an edge-cloud infrastructure that allows performing streaming analysis at the edge to reduce the quantity of the data to store in permanent memory, to know the health status of the machinery at any point in time, and to discover novel and anomalous behaviors. The collection of the data from multiple machines into a cloud server allows training more accurate diagnostic and prognostic models using a higher amount of data, whose results will serve to predict the health status in real-time at the edge. The so-built PHM system would allow industries to monitor and supervise a machinery network placed in different locations and can thus bring several benefits to both machinery producers and users. After a brief literature review of signal processing, feature extraction, diagnostics, and prognostics, including incremental and semi-supervised approaches for anomaly and novelty detection applied to data streams, a case study is presented. It was conducted on data collected from a test rig and shows the potential of the proposed framework in terms of the ability to detect changes in the operating conditions and abrupt faults and storage memory saving. The outcomes of our work, as well as its major novel aspect, is the design of a framework for a PHM system based on specific requirements that directly originate from the industrial field, together with indications on which techniques can be adopted to achieve such goals.
PICUS: A Pocket-Sized System for Simple and Fast Non-Destructive Evaluation of the Detachments in Ancient Artifacts
Authors: Caliano Giosuè , Mariani Francesca , Calicchia Paola
Published: April 2021

An innovative, robust method has been developed, based on the use of a simple, compact, expressly designed device, named PICUS (the ‘woodpecker’ in ancient Latin), and inspired by the auscultation method carried out by the experts in the field of conservation of cultural heritage. This method entails gently knocking the surface, controlling and measuring the impact time of the stroke’s force, recording the generated sound, comparing the acquired sound with a reference sound by calculating the cross-correlation function, and its maximum, as a measure of the detachment. In a nutshell, it performs an analysis similar to that carried out by a professional who performs a routine examination on the detachments by hand. The experimental apparatus consists of a probe made of an electromechanical percussion element that gently taps the surface producing a sound, a force sensor purposely developed to measure the impact force, and a microphone, all connected with an Arduino-like low cost board, to record and elaborate the sounds and the force sensor signal. The probe XY position on the scene is recognized using an infra-red (IR) system with a low-cost IR camera and an IR light-emitting diode (IR-LED) positioned on the probe. The “tapper” and the microphone replace the hand and the ear of a conservator carrying out a detachment investigation, while the comparison with a reference is the typical mind process of a professional restorer. The result is the fusion of the microphone data and the force sensor data.
Assessment of a Mid-Fidelity Numerical Approach for the Investigation of Tiltrotor Aerodynamics
Authors: Zanotti Alex , Savino Alberto , Palazzi Michele , Tugnoli Matteo , Muscarello Vincenzo
Published: April 2021

The study of the complex aerodynamics that characterise tiltrotors represents a challenge for computational fluid dynamics tools. URANS numerical solvers are typically used to explore the aerodynamic features that characterise the different flight conditions of these aircraft, but their computational cost limits their applications to a few vehicle configurations. The present work explores the capabilities of a new mid-fidelity aerodynamic code that is based on the vortex particle method, DUST, to investigate the performance and flow physics of tiltrotors. With this aim, numerical simulations were performed in DUST while considering XV-15 tiltrotor configurations with increasing complexity. The study started with the investigation of a simpler configuration made up of a single wing and a proprotor. Subsequently, the full aircraft was studied in steady-level flights and its major operating flight conditions were explored—i.e., hover, conversion phase, and cruise. A thorough assessment of the code capabilities was performed by the comparison of the numerical results with high-fidelity Computational Fluid Dynamics (CFD) data. This thorough comparison showed that the mid-fidelity numerical approach implemented in DUST is suitable for capturing the flow physics related to the complex aerodynamic interactions between the proprotors and the wing along with the entire flight envelope of the tiltrotor. Moreover, a good representation of the aerodynamic performance of the vehicle was obtained, particularly for the flight conditions that are characterised by limited flow separations. The good accuracy obtained for both the performance and flow physics, combined with the relatively lower computational costs required by the mid-fidelity solver with respect to the URANS simulations, indicates that DUST could be considered a valuable tool for use in the preliminary design of novel tiltrotor configurations.
Airborne Electromagnetics to Improve Landslide Knowledge in Tropical Volcanic Environments
Authors: Thiery Yannick , Reninger Pierre-Alexandre , Nachbaur Aude
Published: April 2021

Caribbean areas are particular volcanic territories in tropical environments. These territories juxtapose several landslide-prone areas with different predisposing factors (poorly consolidated volcanic materials, superimposition of healthy materials on highly weathered materials, high heterogeneity of thicknesses, etc.). In these environments, where rapid development of slopes and land use changes are noticeable, it is necessary to better characterize these unstable phenomena that cause damage to infrastructure and people. This characterization has to be carried out on the materials as well as on the initiation conditions of the phenomena and requires complementary investigations. This study, focusing on La Martinique, proposes a landslide analysis methodology that combines new information about landslide-prone materials acquired by an airborne electromagnetics survey with a physical-based model. Once the data are interpreted and compared with field observations and previous data, a geological model is produced and introduced into the physical model to test different instability scenarios. The results show that geophysical investigations (i) improve the knowledge of the internal structure of landslides and surficial formations, (ii) specify the spatial limits of the materials that are sensitive to landslides, and (iii) give a better understanding of landslide initiation conditions, particularly hydrogeological triggering conditions.
Modulated Responses of East Asian Winter Climate to Anthropogenic Aerosols by Urban Cover in Eastern China
Authors: Deng Jiechun , Zhang Leying , Ma Jing , Chyi Dorina
Published: April 2021

The increasing anthropogenic aerosols (AAs) over East Asia have caused significant regional climate responses, but the role of urban land-use changes which occur simultaneously, in altering these AA-induced changes, is not well understood. Here, the modulation of the AAs’ effect on the East Asian winter (November–January) climate by the urban cover in eastern China was investigated using the Community Atmosphere Model version 5.1 coupled with the Community Land Model version 4. Results show that the winter sulfate aerosol burden is higher from central eastern China to southern Japan in the case with the presence of urban cover than in the case without it, resulting from urban-induced circulation changes. Such aerosol changes markedly increase the cloud fraction and precipitation over northern China and the adjacent ocean to the east, especially convection activities around southern Japan. This leads to a cooling effect near the surface over northern China and in the mid-upper troposphere to the east due to aerosol direct and indirect effects. The resulting circulation responses act to shift the mid-tropospheric East Asian trough southward and the upper-level East Asian westerly jet-stream as well, further supporting the surface changes. These winter climate responses to the urban-modulated aerosols can largely offset or even reverse those to the AAs forcing without the urban cover in the model, especially in northern East Asia. This study highlights the need to consider the modulating role of urban land-use changes in assessing the AAs’ climatic effect over East Asia and other regions.
Evaluation of the Performance of CMIP6 Models in Reproducing Rainfall Patterns over North Africa
Authors: Babaousmail Hassen , Hou Rongtao , Ayugi Brian , Ojara Moses , Ngoma Hamida , Karim Rizwan , Rajasekar Adharsh , Ongoma Victor
Published: April 2021

This study assesses the performance of historical rainfall data from the Coupled Model Intercomparison Project phase 6 (CMIP6) in reproducing the spatial and temporal rainfall variability over North Africa. Datasets from Climatic Research Unit (CRU) and Global Precipitation Climatology Centre (GPCC) are used as proxy to observational datasets to examine the capability of 15 CMIP6 models’ and their ensemble in simulating rainfall during 1951–2014. In addition, robust statistical metrics, empirical cumulative distribution function (ECDF), Taylor diagram (TD), and Taylor skill score (TSS) are utilized to assess models’ performance in reproducing annual and seasonal and monthly rainfall over the study domain. Results show that CMIP6 models satisfactorily reproduce mean annual climatology of dry/wet months. However, some models show a slight over/under estimation across dry/wet months. The models’ overall top ranking from all the performance analyses ranging from mean cycle simulation, trend analysis, inter-annual variability, ECDFs, and statistical metrics are as follows: EC-Earth3-Veg, UKESM1-0-LL, GFDL-CM4, NorESM2-LM, IPSL-CM6A-LR, and GFDL-ESM4. The mean model ensemble outperformed the individual CMIP6 models resulting in a TSS ratio (0.79). For future impact studies over the study domain, it is advisable to employ the multi-model ensemble of the best performing models.
In Vivo and In Vitro Characterization of Primary Human Liver Macrophages and Their Inflammatory State
Authors: Zimmermann Andrea , Hänsel René , Gemünden Kilian , Kegel-Hübner Victoria , Babel Jonas , Bläker Hendrik , Matz-Soja Madlen , Seehofer Daniel , Damm Georg
Published: April 2021

Liver macrophages (LMs) play a central role in acute and chronic liver pathologies. Investigation of these processes in humans as well as the development of diagnostic tools and new therapeutic strategies require in vitro models that closely resemble the in vivo situation. In our study, we sought to gain further insight into the role of LMs in different liver pathologies and into their characteristics after isolation from liver tissue. For this purpose, LMs were characterized in human liver tissue sections using immunohistochemistry and bioinformatic image analysis. Isolated cells were characterized in suspension using FACS analyses and in culture using immunofluorescence staining and laser scanning microscopy as well as functional assays. The majority of our investigated liver tissues were characterized by anti-inflammatory LMs which showed a homogeneous distribution and increased cell numbers in correlation with chronic liver injuries. In contrast, pro-inflammatory LMs appeared as temporary and locally restricted reactions. Detailed characterization of isolated macrophages revealed a complex disease dependent pattern of LMs consisting of pro- and anti-inflammatory macrophages of different origins, regulatory macrophages and monocytes. Our study showed that in most cases the macrophage pattern can be transferred in adherent cultures. The observed exceptions were restricted to LMs with pro-inflammatory characteristics.
Probing Conformational Dynamics by Protein Contact Networks: Comparison with NMR Relaxation Studies and Molecular Dynamics Simulations
Authors: Chasapis Christos T. , Vlamis-Gardikas Alexios
Published: April 2021

Protein contact networks (PCNs) have been used for the study of protein structure and function for the past decade. In PCNs, each amino acid is considered as a node while the contacts among amino acids are the links/edges. We examined the possible correlation between the closeness centrality measure of amino acids within PCNs and their mobility as known from NMR spin relaxation experiments and molecular dynamic (MD) simulations. The pivotal observation was that plasticity within a protein stretch correlated inversely to closeness centrality. Effects on protein conformational plasticity caused by the formation of disulfide bonds or protein–protein interactions were also identified by the PCN analysis measure closeness centrality and the hereby introduced percentage of closeness centrality perturbation (% CCP). All the comparisons between PCN measures, NMR data, and MDs were performed in a set of proteins of different biological functions and structures: the core protease domain of anthrax lethal factor, the N-terminal RING domain of E3 Ub ligase Arkadia, the reduced and oxidized forms of human thioredoxin 1, and the ubiquitin molecules (Ub) of the catalytic Ub–RING–E3–E2–Ub complex of E3 ligase Ark2.The graph theory analysis of PCNs could thus provide a general method for assessing the conformational dynamics of free proteins and putative plasticity changes between different protein forms (apo/complexed or reduced/oxidized).
Development of an Optical Method for the Evaluation of Whole Blood Coagulation
Authors: Louka Marinos , Kaliviotis Efstathios
Published: April 2021

Blood coagulation is a defense mechanism, which is activated in case of blood loss, due to vessel damage, or other injury. Pathological cases arise from malfunctions of the blood coagulation mechanism, and rapid growth of clots results in partially or even fully blocked blood vessel. The aim of this work is to characterize blood coagulation, by analyzing the time-dependent structural properties of whole blood, using an inexpensive design and robust processing approaches. The methods used in this work include brightfield microscopy and image processing techniques, applied on finger-prick blood samples. The blood samples were produced and directly utilized in custom-made glass microchannels. Color images were captured via a microscopy-camera setup for a period of 35 min, utilizing three different magnifications. Statistical information was extracted directly from the color components and the binary conversions of the images. The main advantage in the current work lies on a Boolean classification approach utilized on the binary data, which enabled to identify the interchange between specific structural elements of blood, namely the red blood cells, the plasma and the clotted regions, as a result of the clotting process. Coagulation indices produced included a bulk coagulation index, a plasma-reduction based index and a clot formation index. The results produced with the inexpensive design and the low computational complexity in the current approach, show good agreement with the literature, and a great potential for a robust characterization of blood coagulation.