bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on Joint Recommendation Algorithm for Knowledge Concepts and Learning Partners Based on Improved Multi-Gate Mixture-of-Experts
Authors: Shou Zhaoyu , Chen Yixin , Wen Hui , Liu Jinghua , Mo Jianwen , Zhang Huibing
Published: March 2024

The rise of Massive Open Online Courses (MOOCs) has increased the large audience for higher education. Different learners face different learning difficulties in the process of online learning. In order to ensure the quality of teaching, online learning resource recommendation services should be more personalised and have more choices. In this paper, we propose a joint recommendation algorithm for knowledge concepts and learning partners based on improved MMoE (Multi-gate Mixture-of-Experts). Firstly, the heterogeneous information network (HIN) is constructed based on the MOOC platform and appropriate meta-paths are selected in order to extract the human–computer interaction information and student–student interaction information generated during the learners’ online learning processes more completely. Secondly, the temporal behavioural characteristics of students are obtained based on their learning paths as well as their knowledge of conceptual characteristics, and LSTM (Long Short-Term Memory) is used to mine students’ current learning interests. Finally, the gating network in MMoE is changed into an attention mechanism network, and for different tasks, multiple attention mechanism networks are used to fuse the learner’s human–computer interaction information, student–student interaction information, and interest characteristics to generate learner representations that are more in line with the respective task and to complete the tasks of knowledge conception and learning partner recommendation. Experiments on publicly available MOOC datasets show that the method proposed in this paper provides more accurate and varied personalization services to online learners compared to the latest proposed methods.
Research on Compliance Control of Electro-Hydraulic Loading Experimental System
Authors: Jiang Shuai , Shen Haikuo , Zhi Shaodan , Cheng Chao , Ren Huimin , Tong Jiang
Published: March 2024

This article discusses the challenges in preventing workpiece damage due to impacts in electro-hydraulic loading systems, especially in unknown environments. We propose an innovative compliance control strategy, synergizing a series elastic actuator with impedance control to significantly mitigate impact forces between the mechanism and test workpieces. The controller consists of two loops: an internal loop and an outer loop. The internal loop integrates a position loop utilizing a radial basis function observer within a backstepping control framework, effectively countering the nonlinear dynamics of hydraulic actuators and ensuring precise trajectory tracking. The outer loop advances traditional impedance control by adaptively modifying the damping coefficient, resulting in a straightforward and easily implementable damping control law. For the unknown environment parameters, our system employs a parameter estimation law to estimate the unknown environmental stiffness and position parameters. The effectiveness of this strategy has been verified through comparative simulation with traditional impedance control, indicating that the proposed method can not only effectively reduce contact shock in unknown environments, improve response speed, and reduce overshoot, but also improve steady-state accuracy. We provided a feasible control scheme for similar systems to ensure precise and safe operation.
Energy Efficient Wireless Signal Detection: A Revisit through the Lens of Approximate Computing
Authors: Kulkarni Abhinav , Ouameur Messaoud Ahmed , Massicotte Daniel
Published: March 2024

In the pursuit of energy efficiency in next-generation communication systems, approximate computing is emerging as a promising technique. In the proposed work, efforts are made to address the challenge of bridging the gap between the level of approximation and the Quality-of-Service (QoS) of the system. The application of approximate multiplication to wireless signal detection is explored systematically, illustrated by employing Truncated Multiplication (TM) on Quadrature Phase Shift Keying (QPSK) Minimum Mean Square Error (MMSE) detection. The irregularities induced by approximation in the multiplication operation employed in wireless signal detection are captured by the Approximate Multiplication Noise (AMN) model, which aids in the analysis of signal fidelity and resiliency of the system. The energy efficiency gains through approximation are highlighted in the approximation analysis. Signal fidelity analysis provides the capability to predict system output for varying levels of approximation, which aids in improving the stability of the system. The higher approximation levels are advantageous in low Signal-to-Noise Ratio (SNR) regimes, whereas lower approximation levels prove beneficial in high SNR regimes.
Small-Signal Stability of Hybrid Inverters with Grid-Following and Grid-Forming Controls
Authors: Ji Xiaotong , Liu Dan , Jiang Kezheng , Zhang Zhe , Yang Yongheng
Published: March 2024

In the modern power grid, characterized by the increased penetration of power electronics and extensive utilization of renewable energy, inverter-based power plants play a pivotal role as the principal interface of renewable energy sources (RESs) and the grid. Considering the stability characteristics of grid-following (GFL) inverters when the grid is relatively weak, the application of grid-forming (GFM) controls becomes imperative in enhancing the stability of the entire power plant. Thus, there is an urgent need for suitable and effective models to study the interaction and stability of the paralleled inverters employing GFL and GFM controls. Thus, the small-signal modeling with full-order state-space model and eigenvalues analysis are presented in this paper. First, the small-signal state-space model of the individual GFL and GFM inverters is obtained, considering the control loop, interaction, reference frame, transmissions, and time delays. Then, the models of the individual inverter are extended to the hybrid inverters to study the effects of the GFM inverters on the small-signal stability of the entire system. And the impacts of the inertia and damping are analyzed by the eigenvalues of the state-transition matrix. A case comprising three parallel GFL inverters and two GFL inverters with one GFM inverter, respectively, are studied to examine the effectiveness and accuracy of the model. Finally, the stability margin obtained from the eigenvalue analysis of the entire system is verified by time-domain simulations.
An Experimental Analysis of Three-Phase Low-Voltage Power Factor Controllers Used in a Deforming Regime
Authors: Diniș Corina Maria , Popa Gabriel Nicolae
Published: March 2024

In industry, to improve the power factor in low-voltage power substations, a power factor controller (the most used method) can be installed which connects capacitors banks (connected, or not, with coils) in the electrical installation. The most important parameters of power engineering are the power factors that indicate the efficiency of energy use. Currently, many non-linear consumers (more single-phase than three-phase) are used on low voltages. Harmonics (currents and/or voltages) are the most important dynamic component of power quality, affecting electrical equipment performance and also reducing power factors. The purpose of this analysis is to increase the displacement power factor and to decrease the total harmonic distortion (for the current) in the conditions where there are linear and non-linear consumers, where the power factor must be improved with capacitors banks. Relevant different consumers have been selected for both the industry and the home sector, as follows: inductive motors that are linear, inductive consumers, compact fluorescent lamps that are non-linear, and capacitive consumers. This analysis was carried out depending on the number of steps used for the power factor controller, the values of the capacitors banks, the AC reactor (connected in series with all consumers), and the LC shunt filters. For a slight deformation regime, a large number of capacitors banks with different values ensure a finer adjustment of the displacement factor. The maximum number of steps that regulators can command should not necessarily be used (the reliability of the installation decreases with the increase in the number of capacitors banks), but a reduced number of steps can be used, which can lead to higher values of displacement power factors. To improve the deforming regime and to increase the displacement power factor, the use of LC shunt filters, connected to a small number of steps, will also increase the displacement power factor (over 0.9) and decrease the total harmonic distortion (up to 7–10%) for the current. Weaker results were obtained with AC reactors connected to the power supply phases of consumers and, if a larger number of stages were used, to which LC shunt filters were connected, these filters become difficult to calibrate (resonances occur).
Precipitation Variations in the Central Qilian Mountains since the 7th Century and Regional Differences: Evidence from Tree-Ring Data
Authors: Zhang Taibang , Zhang Yong , Shao Xuemei , Fang Xiuqi
Published: March 2024

The Qilian Mountains, located in northwest China and serving as a crucial water recharge area, have exhibited significant regional differences in precipitation patterns in recent decades. However, the limited temporal coverage of instrumental data has hindered a deep understanding of hydroclimate variations and regional differences. Further investigation into their long-term spatial and temporal precipitation characteristics is urgently needed. In this study, a new tree-ring-width chronology spanning 1743 years was established in the central Qilian Mountains using Qilian juniper (Juniperus przewalskii Kom.) samples. Significant correlations were found between the tree-ring indices and precipitation during both the growing and pre-growing seasons. Based on these correlations, annual precipitation from August of the previous year to July of the current year was reconstructed. The reconstruction model successfully explains 34.5% of the variation in precipitation during the calibration period. The analysis of the reconstructed series reveals notable interannual to multi-decadal dry–wet variability during the period from 614 AD to 2016 AD. The mid- to late-15th century emerges as the longest-lasting dry period, while the last decade stands out as the wettest. Comparative analysis with other precipitation reconstructions in the eastern and western Qilian Mountains reveals that regional drought events tend to be more pronounced and enduring. Low-frequency fluctuations on decadal to century scales show distinct wet and dry periods in the 12th–18th centuries in both the eastern and western parts of the Qilian Mountains, with weaker fluctuations in subsequent centuries. However, the central part of the Qilian Mountains exhibits opposite trends, possibly due to the complex interactions of multiple circulation systems.
Accurate Recognition of Jujube Tree Trunks Based on Contrast Limited Adaptive Histogram Equalization Image Enhancement and Improved YOLOv8
Authors: Ling Shunkang , Wang Nianyi , Li Jingbin , Ding Longpeng
Published: March 2024

The accurate recognition of tree trunks is a prerequisite for precision orchard yield estimation. Facing the practical problems of complex orchard environment and large data flow, the existing object detection schemes suffer from key issues such as poor data quality, low timeliness and accuracy, and weak generalization ability. In this paper, an improved YOLOv8 is designed on the basis of data flow screening and enhancement for lightweight jujube tree trunk accurate detection. Firstly, the key frame extraction algorithm was proposed and utilized to efficiently screen the effective data. Secondly, the CLAHE image data enhancement method was proposed and used to enhance the data quality. Finally, the backbone of the YOLOv8 model was replaced with a GhostNetv2 structure for lightweight transformation, also introducing the improved CA_H attention mechanism. Extensive comparison and ablation results show that the average precision of the quality-enhanced dataset over that of the original dataset increases from 81.2% to 90.1%, and the YOLOv8s-GhostNetv2-CA_H model proposed in this paper reduces the model size by 19.5% compared to that of the YOLOv8s base model, with precision increasing by 2.4% to 92.3%, recall increasing by 1.4%, mAP@0.5 increasing by 1.8%, and FPS being 17.1% faster.
Root Distribution and Water Uptake Applied by Hydrogen and Oxygen Stable Isotopes for Lianas in Northwest China
Authors: Wang Shangtao , Zhu Gaofeng , Bai Wenwen , Yuan Riping , Zhang Yang
Published: March 2024

The root distribution and water uptake of irrigated vines (Vitis vinifera L.) in arid oasis areas remain poorly understood, particularly in terms of the variations in water uptake resulting from plant type and growing period. In this study, excavation and soil coring were employed to investigate the root distribution of vines. Meanwhile, the water uptake dynamics of irrigated vines in an arid oasis area were developed using stable isotopes of hydrogen and oxygen in water bodies (δD and δ18O) and in crops and soil water, coupled with a direct inference approach and a linear mixed model (IsoSource). The soil layers were divided into nine layers via hierarchical cluster analysis. The results indicated that the vertical distributions of the total and fine roots of grapevines were mainly in the range of 40–160 cm, accounting for 93.1% (91.2%) and 92.5% (90.0%) of the total root (200 cm) distribution during May and October, respectively. In the horizontal direction, both the total root and fine root systems were mainly distributed within 0–100 cm from the trunk and contributed 81.2% and 79.8% of the total root distribution, respectively. Meanwhile, both the total root weight (length) density and fine root weight (length) density decreased gradually with increasing radial distance from the trunk in the 0–80 cm range. The main water uptake of vines was at 0–40 cm in June, 20–80 cm in July, and 40–100 cm in August. These findings suggest that the main depth of root water uptake increases during the whole growth stage for grapevines in arid oasis ecosystems. This information will help growers to improve irrigation efficiency and provide a data analysis of water conservation in an arid oasis area during water stress.
Spirulina (Arthrospira platensis): Antiallergic Agent or Hidden Allergen? A Literature Review
Authors: Gromek Weronika , Kołdej Natalia , Kurowski Marcin , Majsiak Emilia
Published: March 2024

Presently, there has been an increase in the consumption of the blue–green microalga–spirulina (Arthrospira species), which dominates 99.5% of the total world production of microalgae. Primarily sold as a dietary supplement, it is also incorporated into snacks, pasta, cookies, and bread. Owing to its nutrient abundance, spirulina has a variety of potential applications. Extensive studies have been conducted on the health benefits of spirulina, but its safety in terms of allergy has received limited attention. Therefore, to bridge this knowledge deficit, this review aimed to evaluate the allergenic and antiallergic potential of spirulina. In the PubMed and Scopus databases using words related to allergy, we attempted to detect papers on hypersensitivity to spirulina. A total of 128 records were identified, of which 49 were screened. Ultimately, in this review, we analyzed four case studies, encompassing a total of five patients with allergies to spirulina. We assessed the severity of allergic reactions following World Allergy Organization (WAO) Anaphylaxis Guidance 2020, which varied from mild (grade 2) to severe (grade 4) based on the patient’s symptoms. Additionally, our findings indicate that allergy to spirulina is not commonly reported or diagnosed. However, most of the described cases (four of five) regarding allergy to spirulina according to WAO Anaphylaxis Guidance 2020 were classified as anaphylaxis. Furthermore, it is noteworthy that spirulina also possesses antiallergic properties, as evidenced by research studies. Our article delves into both the allergic and antiallergic potential of spirulina.
Exploring Non-Thermal Plasma and UV Radiation as Biofilm Control Strategies against Foodborne Filamentous Fungal Contaminants
Authors: Kulišová Markéta , Rabochová Michaela , Lorinčík Jan , Brányik Tomáš , Hrudka Jan , Scholtz Vladimír , Jarošová Kolouchová Irena
Published: March 2024

In recent years, non-thermal plasma (NTP) has emerged as a promising tool for decontamination and disinfection within the food industry. Given the increasing resistance of microbial biofilms to conventional disinfectants and their adverse environmental effects, this method has significant potential for eliminating biofilm formation or mitigating the metabolic activity of grown biofilms. A comparative study was conducted evaluating the efficacy of UV radiation and NTP in eradicating mature biofilms of four common foodborne filamentous fungal contaminants: Alternaria alternata, Aspergillus niger, Fusarium culmorum, and Fusarium graminearum. The findings reveal that while UV radiation exhibits variable efficacy depending on the duration of exposure and fungal species, NTP induces substantial morphological alterations in biofilms, disrupting hyphae, and reducing extracellular polymeric substance production, particularly in A. alternata and F. culmorum. Notably, scanning electron microscopy analysis demonstrates significant disruption of the hyphae in NTP-treated biofilms, indicating its ability to penetrate the biofilm matrix, which is a promising outcome for biofilm eradication strategies. The use of NTP could offer a more environmentally friendly and potentially more effective alternative to traditional disinfection methods.
Development and Characterization of a Hand Rub Gel Produced with Artisan Alcohol (Puntas), Silver Nanoparticles, and Saponins from Quinoa
Authors: Analuiza Oscar , Paredes Belen , Lascano Alejandra , Bonilla Santiago , Martínez-Guitarte José-Luis
Published: March 2024

The emergence of the global pandemic (COVID-19) has directed global attention towards the importance of hygiene as the primary defense against various infections. In this sense, one of the frequent recommendations of the World Health Organization (WHO) is regular hand washing and the use of alcohol-based hand sanitizers. Ethanol is the most widely used alcohol due to its effectiveness in eliminating pathogens, ease of use, and widespread production. However, artisanal alcohol, generally used as a spirit drink, could be a viable alternative for developing sanitizing gels. In this study, the use of alcohol “Puntas”, silver nanoparticles, and saponins from quinoa was evaluated to produce hand sanitizer gels. The rheological, physicochemical, and antimicrobial properties were evaluated. In the previous assays, the formulations were adjusted to be similar in visual viscosity to the control gel. A clear decrease in the apparent viscosity was observed with increasing shear rate, and an inversely proportional relationship was observed with the amount of ethyl alcohol used in the formulations. The flow behavior index (n) values reflected a pseudoplastic behavior. Oscillatory dynamic tests were performed to analyze the viscoelastic behavior of gels. A decrease in storage modulus (G′) and an increase in loss modulus (G″) as a function of the angular velocity (ω) was observed. The evaluation of pH showed that the gels complied with the requirements to be in contact with the skin of the people, and the textural parameters showed that the control gel was the hardest. The use of artisan alcohol could be an excellent alternative to produce sanitizer gel and contribute to the requirements of the population.
Gene Dosage of F5 c.3481C>T Stop-Codon (p.R1161Ter) Switches the Clinical Phenotype from Severe Thrombosis to Recurrent Haemorrhage: Novel Hypotheses for Readthrough Strategy
Authors: Gemmati Donato , D’Aversa Elisabetta , Antonica Bianca , Grisafi Miriana , Salvatori Francesca , Pizzicotti Stefano , Pellegatti Patrizia , Ciccone Maria , Moratelli Stefano , Serino Maria Luisa , Tisato Veronica
Published: March 2024

Inherited defects in the genes of blood coagulation essentially express the severity of the clinical phenotype that is directly correlated to the number of mutated alleles of the candidate leader gene (e.g., heterozygote vs. homozygote) and of possible additional coinherited traits. The F5 gene, which codes for coagulation factor V (FV), plays a two-faced role in the coagulation cascade, exhibiting both procoagulant and anticoagulant functions. Thus, defects in this gene can be predisposed to either bleeding or thrombosis. A Sanger sequence analysis detected a premature stop-codon in exon 13 of the F5 gene (c.3481C>T; p.R1161Ter) in several members of a family characterised by low circulating FV levels and contrasting clinical phenotypes. The propositus, a 29 y.o. male affected by recurrent haemorrhages, was homozygous for the F5 stop-codon and for the F5 c.1691G>A (p.R506Q; FV-Leiden) inherited from the heterozygous parents, which is suggestive of combined cis-segregation. The homozygous condition of the stop-codon completely abolished the F5 gene expression in the propositus (FV:Ag < 1%; FV:C < 1%; assessed by ELISA and PT-based one-stage clotting assay respectively), removing, in turn, any chance for FV-Leiden to act as a prothrombotic molecule. His father (57 y.o.), characterised by severe recurrent venous thromboses, underwent a complete molecular thrombophilic screening, revealing a heterozygous F2 G20210A defect, while his mother (56 y.o.), who was negative for further common coagulation defects, reported fully asymptomatic anamnesis. To dissect these conflicting phenotypes, we performed the ProC®Global (Siemens Helthineers) coagulation test aimed at assessing the global pro- and anticoagulant balance of each family member, investigating the responses to the activated protein C (APC) by means of an APC-sensitivity ratio (APC-sr). The propositus had an unexpectedly poor response to APC (APC-sr: 1.09; n.v. > 2.25), and his father and mother had an APC-sr of 1.5 and 2.0, respectively. Although ProC®Global prevalently detects the anticoagulant side of FV, the exceptionally low APC-sr of the propositus and his discordant severe–moderate haemorrhagic phenotype could suggest a residual expression of mutated FV p.506QQ through a natural readthrough or possible alternative splicing mechanisms. The coagulation pathway may be physiologically rebalanced through natural and induced strategies, and the described insights might be able to track the design of novel treatment approaches and rebalancing molecules.
Validation and Clinical Application of the Japanese Version of the Patient-Reported Experience Measures for Intermediate Care Services: A Cross-Sectional Study
Authors: Goto Yuko , Miura Hisayuki
Published: March 2024

Older adult patients account for 70% of all hospitalized patients in Japan, and intermediate care based on patient-centered care (PCC) that ensures continuity and quality of care at the interface between home services and acute care services and restores patient’s independence and confidence is necessary for them to continue living independently. At present, no concept of intermediate care is established in Japan, and the implementation of PCC has been delayed. Thus, in this study, a Japanese version of the intermediate care evaluation index (patient-reported experience measure (PREM)) was created on the basis of the original PREM developed in the UK, and data in wards with intermediate care functions in Japan were collected to confirm internal consistency and validity from 2020 to 2022. The Japanese version of PREM was found to have a factor structure with two potential factors. Given the clear correlation with the shared decision-making evaluation index, which is the pinnacle of PCC, the theoretical validity of the Japanese version of PREM, which is based on PCC as a theoretical basis, was confirmed.
Risk of Eating Disorders and Social Desirability among Higher Education Students: Comparison of Nutrition Students with Other Courses
Authors: Fernandes Sandra , Costa Carolina , Nakamura Ingrid Sayumi , Poínhos Rui , Oliveira Bruno M. P. M.
Published: March 2024

The transition to college is a period of higher risk of the development of eating disorders, with nutrition/dietetics students representing a group of particular vulnerability. Hence, it is interesting to assess eating disorders, taking into consideration potential sources of bias, including social desirability. Our aims were to compare the risk of eating disorders between students of nutrition/dietetics and those attending other courses and to study potential social desirability biases. A total of 799 higher education students (81.7% females) aged 18 to 27 years old completed a questionnaire assessing the risk of eating disorders (EAT-26) and social desirability (composite version of the Marlowe–Crowne Social Desirability Scale). The proportion of students with a high risk of eating disorders was higher among females (14.5% vs. 8.2%, p = 0.044). Nutrition/dietetics students did not differ from those attending other courses regarding the risk of eating disorders. The social desirability bias when assessing the risk of eating disorders was overall low (EAT-26 total score: r = −0.080, p = 0.024). Social desirability correlated negatively with the Diet (r = −0.129, p < 0.001) and Bulimia and food preoccupation subscales (r = −0.180, p < 0.001) and positively with Oral self-control (r = 0.139, p < 0.001).
Optimizing ‘Xinomavro’ (Vitis vinifera L.) Performance by Post-Bloom Basal Leaf Removal Applications
Authors: Theocharis Serafeim , Taskos Dimitrios , Gkrimpizis Theodoros , Nikolaou Kleopatra-Eleni , Miliordos Dimitrios-Evangelos , Koundouras Stefanos
Published: March 2024

A three-year study was conducted to investigate the effects of two timings—at berry set and at veraison—of post-bloom leaf removal (LR) applications from the basal sections of the shoot on the growth, yield, and berry composition of Vitis vinifera L. cv. Xinomavro, the major red winegrape of North Greece. LR at berry set drastically reduced yield by affecting all its components while increasing the ratio of secondary foliage per total leaf area. LR at veraison had a milder effect on growth and yield. Both treatments increased sugar and phenolic compound levels in berries, while early LR increased the contribution of more stable forms of anthocyanins. Additionally, early LR increased the skin-to-pulp ratio, suggesting that it could be an effective viticultural technique for more concentrated red wines. Overall, both LR timings improved most of the berry attributes compared to the non-treated vines; however, the earlier application can be recommended when aiming at the production of deep-colored and richly flavored wines.
Analysis of the Impact of Hydraulic Gates on a Stabilized Tidal Inlet Structure: Mathematical Model and Data Measurements
Authors: Arrieta-Pastrana Alfonso , Coronado-Hernández Oscar E. , Fuertes-Miquel Vicente S.
Published: March 2024

Tidal inlet structures are engineering projects with associated benefits related to flood control, water quality enhancement, and coastal protection. This study analyzes the performance of hydraulic gates on a stabilized inlet in estuarine systems by developing a simplified hydraulic model that considers inlet and outlet water levels. The proposed model was applied to the stabilized tidal inlet structure in Cartagena de Indias, Colombia. This model offers a practical tool for engineers and designers operating estuarine systems. The analysis focuses on the coastal lagoon of Ciénaga de la Virgen. The proposed model was successfully calibrated using two water sensors, with extreme input and outlet flow rates of approximately 260 m3/s and 110 m3/s, respectively. The average daily output volume in the system is 3,361,000 m3, while the average daily input volume is 3,200,000 m3. Consequently, the manipulation of the opening gates results in a decrease in the estuarine water level, potentially by as much as 25 cm, which local authorities can use to make decisions to reduce extreme water levels during flooding events.
The Role of Climatic, Environmental and Socioeconomic Factors in the Natural Movement of Urban Populations in Kazakhstan, 2012–2020: An Analysis from a Middle-Income Country in Central Asia
Authors: Smagulov Nurlan , Zhamantayev Olzhas , Konkabayeva Aiman , Adilbekova Ainur , Zhanalina Gulmira , Shintayeva Nurzhamal , Aubakirova Dinara
Published: March 2024

Background: This study addresses the importance of identifying key characteristics influencing demographic indicators for urban populations, emphasizing the need to consider regional climatic features and ecological factors. The research utilized data from ten main regional cities across the Republic of Kazakhstan. Methods: This study involved a retrospective analysis based on secondary data from official sources spanning 2012–2020. We employed correlation analysis and multidimensional regression models. Results: Noteworthy predictors for crude birth rate included the influence of effective temperature (β = 0.842, p < 0.0001), marriage rate (β = 0.780, p < 0.0001), Gini coefficient (β = −27.342, p = 0.020) and divorce rate (β = −2.060, p < 0.0001), with overall strong model performance (R2 = 0.940). The degree of atmospheric pollution (β = −0.949, p = 0.044), effective temperature (β = −0.294, p < 0.0001) and Gini coefficient (β = 19.923, p = 0.015) were the predictors for crude mortality rate, with a high model fit (R2 = 0.796). Conclusions: The study unveils significant relationships between demographic indicators (crude birth rate, mortality rate) and variables like effective temperature, marriage rate, divorce rate, Gini coefficient, physician density and others. This analysis of climatic, environmental, and socioeconomic factors influencing demographic indicators may help in promoting specific measures to address public health issues in Kazakhstan.
Correction: Meh et al. Reliability and Validity of Slovenian Versions of IPAQ-SF, GPAQ, and EHIS-PAQ for Assessing Physical Activity and Sedentarism of Adults. Int. J. Environ. Res. Public Health 2022, 19, 430
Authors: Meh Kaja , Sember Vedrana , Đurić Saša , Vähä-Ypyä Henri , Rocha Paulo , Jurak Gregor
Published: March 2024

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Exploring the Influence of a Physical Activity and Healthy Eating Program on Childhood Well-Being: A Comparative Study in Primary School Students
Authors: Vega-Ramírez Lilyan
Published: March 2024

Childhood is a crucial stage of human development in which the lifestyles children adopt can have a significant impact on their well-being throughout their lives. The aim of this study was to analyze and compare the healthy habits and Body Mass Index (BMI) of students from a primary school that participated in a program to promote physical activity and healthy eating one year earlier with other students from two schools that had not participated in this type of program. We analyzed a sample of 287 Spanish students, aged between 8 and 12 years. A survey of healthy habits was completed, and anthropometric data were taken to determine their Body Mass Index (BMI). The questionnaire data indicated that there are some significant differences (p = ≤ 0.05) in the consumption of some unhealthy foods between the evaluated groups. An amount of 11% of the sample was considered obese and 26% were overweight; no significant differences were found between the groups. This study suggests that the healthy habits strategy implemented by a school improves pupils’ habits, especially in reducing the consumption of unhealthy foods. Despite the positive effects, the data indicate that these programs fall short of government recommendations, particularly in areas such as physical activity and certain dietary choices.
Neurodegeneration Biomarkers in Adult Spinal Muscular Atrophy (SMA) Patients Treated with Nusinersen
Authors: Andrés-Benito Pol , Vázquez-Costa Juan Francisco , Ñungo Garzón Nancy Carolina , Colomina María J. , Marco Carla , González Laura , Terrafeta Cristina , Domínguez Raúl , Ferrer Isidro , Povedano Mónica
Published: March 2024

The objective of this study is to evaluate biomarkers for neurodegenerative disorders in adult SMA patients and their potential for monitoring the response to nusinersen. Biomarkers for neurodegenerative disorders were assessed in plasma and CSF samples obtained from a total of 30 healthy older adult controls and 31 patients with adult SMA type 2 and 3. The samples were collected before and during nusinersen treatment at various time points, approximately at 2, 6, 10, and 22 months. Using ELISA technology, the levels of total tau, pNF-H, NF-L, sAPPβ, Aβ40, Aβ42, and YKL-40 were evaluated in CSF samples. Additionally, plasma samples were used to measure NF-L and total tau levels using SIMOA technology. SMA patients showed improvements in clinical outcomes after nusinersen treatment, which were statistically significant only in walkers, in RULM (p = 0.04) and HFMSE (p = 0.05) at 24 months. A reduction in sAPPβ levels was found after nusinersen treatment, but these levels did not correlate with clinical outcomes. Other neurodegeneration biomarkers (NF-L, pNF-H, total tau, YKL-40, Aβ40, and Aβ42) were not found consistently changed with nusinersen treatment. The slow progression rate and mild treatment response of adult SMA types 2 and 3 may not lead to detectable changes in common markers of axonal degradation, inflammation, or neurodegeneration, since it does not involve large pools of damaged neurons as observed in pediatric forms. However, changes in biomarkers associated with the APP processing pathway might be linked to treatment administration. Further studies are warranted to better understand these findings.
More than Just Bread and Wine: Using Yeast to Understand Inherited Cytochrome Oxidase Deficiencies in Humans
Authors: Caron-Godon Chenelle A. , Collington Emma , Wolf Jessica L. , Coletta Genna , Glerum D. Moira
Published: March 2024

Inherited defects in cytochrome c oxidase (COX) are associated with a substantial subset of diseases adversely affecting the structure and function of the mitochondrial respiratory chain. This multi-subunit enzyme consists of 14 subunits and numerous cofactors, and it requires the function of some 30 proteins to assemble. COX assembly was first shown to be the primary defect in the majority of COX deficiencies 36 years ago. Over the last three decades, most COX assembly genes have been identified in the yeast Saccharomyces cerevisiae, and studies in yeast have proven instrumental in testing the impact of mutations identified in patients with a specific COX deficiency. The advent of accessible genome-wide sequencing capabilities has led to more patient mutations being identified, with the subsequent identification of several new COX assembly factors. However, the lack of genotype–phenotype correlations and the large number of genes involved in generating a functional COX mean that functional studies must be undertaken to assign a genetic variant as being causal. In this review, we provide a brief overview of the use of yeast as a model system and briefly compare the COX assembly process in yeast and humans. We focus primarily on the studies in yeast that have allowed us to both identify new COX assembly factors and to demonstrate the pathogenicity of a subset of the mutations that have been identified in patients with inherited defects in COX. We conclude with an overview of the areas in which studies in yeast are likely to continue to contribute to progress in understanding disease arising from inherited COX deficiencies.
Mechanotransduction Circuits in Human Pathobiology
Authors: Gargalionis Antonios N. , Papavassiliou Kostas A. , Papavassiliou Athanasios G.
Published: March 2024

It is widely acknowledged that mechanical forces exerted throughout the human body are critical for cellular and tissue homeostasis [...]
Difference between Keratinized- and Non-Keratinized-Originating Epithelium in the Process of Immune Escape of Oral Squamous Cell Carcinoma
Authors: Kitsukawa Yoshiaki , Fukumoto Chonji , Hyodo Toshiki , Komiyama Yuske , Shiraishi Ryo , Koike Aya , Yagisawa Shuma , Kunitomi Yosuke , Hasegawa Tomonori , Kotani Wataru , Ishida Kazuyuki , Wakui Takahiro , Kawamata Hitoshi
Published: March 2024

Immune checkpoint inhibitors (ICIs), including anti-programmed cell death 1 ligand 1 (PD-L1) antibodies, are significantly changing treatment strategies for human malignant diseases, including oral cancer. Cancer cells usually escape from the immune system and acquire proliferative capacity and invasive/metastatic potential. We have focused on the two immune checkpoints, PD-1/PD-L1 and CD47/SIRPα, in the tumor microenvironment of oral squamous cell carcinoma (OSCC), performed a retrospective analysis of the expression of seven immune-related factors (PD-L1, PD-1, CD4, CD8, CD47, CD56 and CD11c), and examined their correlation with clinicopathological status. As a result, there were no significant findings relating to seven immune-related factors and several clinicopathological statuses. However, the immune checkpoint-related factors (PD-1, PD-L1, CD47) were highly expressed in non-keratinized epithelium-originated tumors when compared to those in keratinized epithelium-originated tumors. It is of interest that immunoediting via immune checkpoint-related factors was facilitated in non-keratinized sites. Several researchers reported that the keratinization of oral mucosal epithelia affected the immune response, but our present finding is the first study to show a difference in tumor immunity in the originating epithelium of OSCC, keratinized or non-keratinized. Tumor immunity, an immune escape status of OSCC, might be different in the originating epithelium, keratinized or non-keratinized.
Formation of DNA Adducts by 1-Methoxy-3-indolylmethylalcohol, a Breakdown Product of a Glucosinolate, in the Mouse: Impact of the SULT1A1 Status—Wild-Type, Knockout or Humanised
Authors: Glatt Hansruedi , Weißenberg Sarah Yasmin , Ehlers Anke , Lampen Alfonso , Seidel Albrecht , Schumacher Fabian , Engst Wolfram , Meinl Walter
Published: March 2024

We previously found that feeding rats with broccoli or cauliflower leads to the formation of characteristic DNA adducts in the liver, intestine and various other tissues. We identified the critical substances in the plants as 1-methoxy-3-indolylmethyl (1-MIM) glucosinolate and its degradation product 1-MIM-OH. DNA adduct formation and the mutagenicity of 1-MIM-OH in cell models were drastically enhanced when human sulfotransferase (SULT) 1A1 was expressed. The aim of this study was to clarify the role of SULT1A1 in DNA adduct formation by 1-MIM-OH in mouse tissues in vivo. Furthermore, we compared the endogenous mouse Sult1a1 and transgenic human SULT1A1 in the activation of 1-MIM-OH using genetically modified mouse strains. We orally treated male wild-type (wt) and Sult1a1-knockout (ko) mice, as well as corresponding lines carrying the human SULT1A1-SULT1A2 gene cluster (tg and ko-tg), with 1-MIM-OH. N2-(1-MIM)-dG and N6-(1-MIM)-dA adducts in DNA were analysed using isotope-dilution UPLC-MS/MS. In the liver, caecum and colon adducts were abundant in mice expressing mouse and/or human SULT1A1, but were drastically reduced in ko mice (1.2–10.6% of wt). In the kidney and small intestine, adduct levels were high in mice carrying human SULT1A1-SULT1A2 genes, but low in wt and ko mice (1.8–6.3% of tg-ko). In bone marrow, adduct levels were very low, independently of the SULT1A1 status. In the stomach, they were high in all four lines. Thus, adduct formation was primarily controlled by SULT1A1 in five out of seven tissues studied, with a strong impact of differences in the tissue distribution of mouse and human SULT1A1. The behaviour of 1-MIM-OH in these models (levels and tissue distribution of DNA adducts; impact of SULTs) was similar to that of methyleugenol, classified as “probably carcinogenic to humans”. Thus, there is a need to test 1-MIM-OH for carcinogenicity in animal models and to study its adduct formation in humans consuming brassicaceous foodstuff.
Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002
Authors: Vincent Marine , Blanc-Garin Victoire , Chenebault Célia , Cirimele Mattia , Farci Sandrine , Garcia-Alles Luis Fernando , Cassier-Chauvat Corinne , Chauvat Franck
Published: March 2024

Terpenes are high-value chemicals which can be produced by engineered cyanobacteria from sustainable resources, solar energy, water and CO2. We previously reported that the euryhaline unicellular cyanobacteria Synechocystis sp. PCC 6803 (S.6803) and Synechococcus sp. PCC 7002 (S.7002) produce farnesene and limonene, respectively, more efficiently than other terpenes. In the present study, we attempted to enhance farnesene production in S.6803 and limonene production in S.7002. Practically, we tested the influence of key cyanobacterial enzymes acting in carbon fixation (RubisCO, PRK, CcmK3 and CcmK4), utilization (CrtE, CrtR and CruF) and storage (PhaA and PhaB) on terpene production in S.6803, and we compared some of the findings with the data obtained in S.7002. We report that the overproduction of RubisCO from S.7002 and PRK from Cyanothece sp. PCC 7425 increased farnesene production in S.6803, but not limonene production in S.7002. The overexpression of the crtE genes (synthesis of terpene precursors) from S.6803 or S.7002 did not increase farnesene production in S.6803. In contrast, the overexpression of the crtE gene from S.6803, but not S.7002, increased farnesene production in S.7002, emphasizing the physiological difference between these two model cyanobacteria. Furthermore, the deletion of the crtR and cruF genes (carotenoid synthesis) and phaAB genes (carbon storage) did not increase the production of farnesene in S.6803. Finally, as a containment strategy of genetically modified strains of S.6803, we report that the deletion of the ccmK3K4 genes (carboxysome for CO2 fixation) did not affect the production of limonene, but decreased the production of farnesene in S.6803.
Changes in Endogenous Essential Metal Homeostasis in the Liver and Kidneys during a Six-Month Follow-Up Period after Subchronic Cadmium Exposure
Authors: Kusak Rafał , Nasiadek Marzenna , Stragierowicz Joanna , Hanke Wojciech , Kilanowicz Anna
Published: March 2024

Cadmium (Cd) is one of the most dangerous environmental pollutants. Its mechanism of action is multidirectional; among other things, it disrupts the balance of key essential elements. The aim of this study was to assess how cumulative exposure to Cd influences its interaction with selected essential elements (Cu, Zn, Ca, and Mg) in the kidney and liver during long-term observation (90 and 180 days) after subchronic exposure of rats (90 days) to Cd at common environmental (0.09 and 0.9 mg Cd/kg b.w.) and higher (1.8 and 4.5 mg Cd/kg b.w.) doses. Cd and essential elements were analyzed using the F-AAS and GF-AAS techniques. It was shown that the highest bioaccumulation of Cd in the kidney occurred six months after the end of exposure, and importantly, the highest accumulation was found after the lowest Cd dose (i.e., environmental exposure). Organ bioaccumulation of Cd (>21 μgCd/g w.w. in the kidney and >6 μgCd/g w.w. in the liver) was accompanied by changes in the other studied essential elements, particularly Cu in both the kidney and liver and Zn in the liver; these persisted for as long as six months after the end of the exposure. The results suggest that the critical concentration in human kidneys (40 μgCd/g w.w.), currently considered safe, may be too high and should be reviewed, as the observed long-term imbalance of Cu/Zn in the kidneys may lead to renal dysfunction.
Locating Electrified Aircraft Service to Reduce Urban Congestion
Authors: Bridgelall Raj
Published: March 2024

The relentless expansion of urban populations and the surge in e-commerce have increased the demand for rapid delivery services, leading to an increase in truck traffic that contributes to urban congestion, environmental pollution, and economic inefficiencies. The critical challenge this poses is not only in managing urban spaces efficiently but also in aligning with global sustainability goals. This study addresses the pressing need for innovative solutions to reduce reliance on truck transportation in congested urban areas without compromising the efficiency of freight delivery systems. This study contributes a novel approach that leverages electrified and autonomous aircraft (EAA) cargo shuttles to shift the bulk of air transportable freight from road to air, specifically targeting underutilized airports and establishing vertiports in remote locations. By applying data mining techniques to analyze freight flow data, this research identifies key commodity categories and metropolitan statistical areas (MSAs) where the implementation of EAA services could significantly mitigate truck-induced congestion. The findings reveal that targeting a select few commodities and MSAs can potentially decrease truck traffic, with electronics emerging as the dominant commodity category, and cities like Los Angeles and Chicago as prime candidates for initial EAA service deployment. Stakeholders in urban planning, transportation logistics, and environmental policy will find this study’s insights beneficial. This work lays a foundation for future innovations in sustainable urban mobility and logistics.
Versatile Machine Learning-Based Authentications by Using Enhanced Time-Sliced Electrocardiograms
Authors: Zhao Yi , Kim Song-Kyoo
Published: March 2024

This paper addresses the enhancement of modern security through the integration of electrocardiograms (ECGs) into biometric authentication systems. As technology advances, the demand for reliable identity authentication systems has grown, given the rise in breaches associated with traditional techniques that rely on unique biological and behavioral traits. These techniques are emerging as more reliable alternatives. Among the biological features used for authentication, ECGs offer unique advantages, including resistance to forgery, real-time detection, and continuous identification ability. A key contribution of this work is the introduction of a variant of the ECG time-slicing technique that outperforms existing ECG-based authentication methods. By leveraging machine learning algorithms and tailor-made compact data learning techniques, this research presents a more robust, reliable biometric authentication system. The findings could lead to significant advancements in network information security, with potential applications across various internet and mobile services.