bluuebunny/crossref_metadata_2025 huggingface dataset:
Excretions of Urinary Creatinine on Young and Mature Kacang Goat under Different Feeding Levels
Authors: Mukminah Nurul , Rianto Edy , Purbowati Endang
Published: February 2017

Abstract. This study was aimed to examine the excretion of urinary creatinine in young and mature Kacang goat bucks under different feeding levels. This study used 16 Kacang goat bucks consisting of 2 groups of age, i.e. eight young bucks (aged 6-7 months, weighed 12.75±2.68 kg) and 8 mature bucks (age 9-12 months, weighed 17.34±3.32 kg). The bucks were fed pelleted complete feed containing 78.82% dry matter (DM), 18.80% crude protein (CP), and 76.29% total digestible nutrients (TDN). The bucks were allocated into a 2x2 nested design with four replications. The treatment was the amount of  2.24% dry matter intake  (T1) and 4.48% of body weight (BW) (T2) for the young goat, while the mature buck was 1.87% and 3.74%, respectively. The results showed that DM, CP and TDN intake were significant different across ages and highly significantly different between feeding levels. Changes of urinary creatinine from week 0–12 showed no differences in the age group (142 mg/dl) and feeding level (143 mg/dl). Conclusively, age and feed level affected body weight, feed intake and creatinine excretion of Kacang Goat.  The more body weight gain (age) and feed level, the more urinal creatinine excretion in male Kacang goat. Key words: Kacang goat, ages, feeding level, and creatinine Abstrak. Penelitian ini bertujuan untuk mengkaji kadar kreatinin pada kambing Kacang muda dan dewasa dengan jumlah pemberian pakan yang berbeda. Materi berupa 16 ekor kambing Kacang jantan, terdiri dari 8 ekor umur muda (6-7 bulan) dan 8 ekor umur dewasa (9-12 bulan). Pakan komplit yang diberikan memiliki kandungan bahan kering (BK) 78,82%, protein kasar (PK) 18,80%, dan total digestible nutrients (TDN) 76,29%. Rancangan penelitian ini adalah pola tersarang 2x2 dengan 4 ulangan. Perlakuan pakan berupa jumlah BK pakan yang diberikan yaitu 2,24% bobot badan (BB) (T1) dan 4,48% BB (T2) untuk kambing muda, sedangkan kambing dewasa sebesar 1,87% BB (T1) dan 3,74% BB (T2). Hasil penelitian menunjukkan bahwa konsumsi BK, PK dan TDN berbeda nyata pada kelompok umur dan berbeda sangat nyata pada perlakuan pakan. Kadar kreatinin pada minggu ke 0-6 tidak berbeda nyata, namun pada minggu ke 12 berbeda nyata baik kelompok umur maupun akibat perlakuan pakan. Perubahan kadar kreatinin dari minggu ke 0-12 menunjukkan perbedaan yang tidak nyata pada kelompok umur dan perlakuan pakan. Kesimpulan dari penelitian ini adalah kadar kreatinin semakin meningkat dengan semakin meningkatnya bobot badan (umur) dan pakan ternak. Kata kunci : kambing Kacang, umur, level pakan, dan kreatinin
Effect of Condensed Tannin of Leucaena and Calliandra Leaves in Protein Trash Fish Silage on In vItro Ruminal Fermentation, Microbial Protein Synthesis and Digestibility
Authors: Rimbawanto Efka Aris , Yusiati Lies Mira , Baliarti Endang , Utomo Ristianto
Published: February 2017

Two plants as condensed tannin (CT) sources were supplemented to protein trash fish silage (TFS) to observe their effect on in vitro ruminal fermentation product, micobial protein synthesis, and   digestibility. CT supplementation on protein trash fish silage was on control proportion (0), under optimum level (2.0%), at optimum level (4.0%), and above optimum level (6.0%) of g TFS); of protein precipitation of bovine serum albumin (BSA) with CT from L. leucocephala; and under optimum level (1.5%), at optimum level (3.0%) and above optimum level (4.5% of g TFS), BSA protein precipitation with CT from C. calothyrsus. The effect on degradation in ruminal fluid and ruminal fluid followed by incubation in HCl-pepsin was evaluated using a modified two-step in vitro method. The CT level of L. leucocephala and C. calothyrsus was 4% and 6%, respectively with protein precipitation BSA was 26.25 and 31.77 g BSA/g CT, respectively. CT supplementation to trash fish silage, ruminal fermentation product (NH3-N, C2, C3, and total VFAs) and digestibility (DM and OM) decreased (P<0.01) and increased (P<0.01) total CP digestibility (in HCl-pepsin). The difference was attributed to CT source without affecting ruminal microbial protein synthesis. CT of L. leucocephala was better in increasing total protein digestibility (70%) than that of C. calothyrsus  (15%). It indicated that CT of C. calothyrsus was less effective in protected TSF protein degradation in rumen compared to that of L. leucocephala.
The Diversity and Productivity of Indigenous Forage in Former Limestone Mining Quarry in Karst Mountain of Southern Gombong, Central Java Indonesia
Authors: Sarwanto Doso , Prayitno Caribu Hadi
Published: February 2017

Indonesia is a country that has a lot of limestone mountains, covering 15.4 million hectares. Limestone mountains have strategic functions as limestone is used as building materials and as raw material in cement industry. Therefore, limestone mining quarry in various areas of limestone mountains in Indonesia is increasingly widespread. The biggest negative impact of limestone mining is the formed open land which is abandoned and unutilized. Changes in the ecosystem will lead to the reduced levels of diversity and productivity of indigenous forage which will ultimately reduce the performance and development of ruminants livestock kept by farmers in the mountainous region of limestone. This study aims to determine the diversity and productivity of indigenous forage on former limestone mining quarry in limestone mountains of southern Gombong. The research was conducted through survey by identifying and measuring the forage production of sample plots assigned purposively. Location of the study was divided into three categories, mild, moderate and heavy mining. Results showed that soil fertility levels in open fields of former limestone mining in southern Gombong mountains are low with total N content of 0.049 - 0.141%, total P2O5 of 0.067 - 0.133% and total K2O of 0.086 - 0.100%. The diversity of indigenous forage on mild mining was more diverse than that of moderate and heavy mining, i.e. 13 species comprising 7 grass species, 2 legumes species, and 4 species of shrubs. The most dominant species in all mining categories are Cynodon dactylon, Imperata cylindrica, Ageratum conyzoides and Mikania micrantha. The results also showed that in the open land of mild mining had the highest production of fresh and dry matter compared to that of moderate and severe mining
Methane Gas Reduction Using Virgin Coconut Oil Supplementation in Rumen Fermentation through in Vitro
Authors: Sondakh Erwin Hubert Barton , Rorong Johnly Alfreds , Kalele Jerry Audy Donny
Published: February 2017

This study aims to determine the effect of virgin coconut oil (VCO)supplementation into ruminant feed on the methane reduction in vitro The study used rumen fluid with forage substrate and concentrates in the ratio of 60:40. The experiement consists of five kinds of VCO treatments, namely: R0 – feed substrates without VCO; R1 – feed substrates with 2% VCO; R2 – feed substrates with 4% VCO; R3 – feed substrates with 6% VCO; and R4 – feed substrates with 8% VCO. Each treatment was done three times. The fermentation used Hohenheim Gas Test (HGT) at a temperature of 390 C for 48 hours. At the end of the fermentation, measurements of methane and microbial activity of the rumen fluid were implemented. Data obtained were analyzed using a completely randomized design, and continued with Duncan test for examining the mean differences. The results showed that methane production descreased by 18.39% - 29.7% when the feed was given 2% - 8% VCO. Based in the findings, it can be concluded that the supplementation of 2% - 8% VCO can be used a substrate for ruminant feed because it can reduce methane and does not interfere the microbial activity of the fermentation of rumen fluid through in vitro.
In Vitro Study of Fermented Complete Feed by Using Sago Residues as Main Source Diet
Authors: Samadi Samadi , Wajizah Sitti , Usman Yunasri
Published: February 2017

Recently, fermentation of low quality feed such as agro industry by products has been widely applied to produce enriched animal feed and improve animal productivity. The purpose of this study was to evaluate in vitro digestibility of fermented complete feed by using agro-residues from sago starch processing industries as main source diet.  Feed was formulated on the basis of 40% sago residue and mixed with other ingredients (rice brand, coconut meal, bread by product, soybean meal and soybean hulls) to fulfill the requirement of sheep with 16,10% of CP, 2,80 Mkal ME/kg and TDN 60,88% based on calculation. Mineral and Urea were added in the complete feed to reach mineral and CP requirements of sheep.  Complete feed formulation was fermented by using 3 commercial fermentation products (Saus Burger Pakan® (SBP), Probion® and EM4®) for 21 days. Total and types of microbes from commercial fermentation products which were utilized in this study were not determined. Parameters measured in this study were pH, NH3, IVDMD (in vitro dry matter digestibility), IVOMD (in vitro organic matter digestibility) and VFA total. The model used for the statistical analysis was completely randomized design (CRD) with 4 treatments (control and 3 different commercial fermentation products) and 4 replications.  The results of this study indicated that administration of different commercial fermentation products into fermented complete feed based on sago residues significantly influenced (P<0.05) on pH, NH3, VFA total, IVDMD and IVOMD. Generally, commercial fermentation products mainly SBP produced better feed quality by improving the values of pH, NH3, VFA total, IVDMD and IVOMD. However, types and total microorganisms were needed to be determined before experiment.
Study on Callus Induction System of 4 Genotype of Napiergrass (Pennisetum purpureum )
Authors: Umami Nafiatul , Akashi Ryo , Gondo Takahiro , Ishigaki Genki , Tanaka Hidenori
Published: February 2017

The aim of this study was to produce callus induction potential of 4 napiergrass (Pennisetum purpureum) genotypes (Dwarf Late, Hybrid, Merkeron and Wrukwona). Callus was induced from shoot apices of shoot tillers  on MS media containing 2,4-D and BAP. On the MS medium containing 2 mg L–1 2,4-D and 0.5 mg L–1 BAP all genotypes could produce embryogenic calli, with different rate of growth. The best genotype for producing embryogenic calli was dwarf napiergrass in 60 day culture. These genotypes would be usefull for tissue cultured based research and for napiergrass improvement program, particularly in genetic transformation. Culturing shoot apices on MS medium containing 2 mgL–1 2,4-D and 0.5 mgL–1 BAP was more suitable than on MS medium containing 0.5 mgL–1 2,4-D.  In the subculture with similar medium composition, proliferation occured poorly on dwarf napiergrass, whereas none happened on the three other genotypes. On the hormon-free medium, all genotypes germinated in different rates. This research pointed out that dwarf napiergrass gave the best response toward induction medium. However, its proliferation and regeneration needed to be optimized in order to obtain more obvious data. This genotype would be usefull for tissue culture based research and for napiergrass improvement program, particularly in genetic transformation.
Evaluation of Performance in Female Madura Cattle in Madura Island, Indonesia
Authors: Nurgiartiningsih Veronica Margareta Ani , Budiarto Agus , Kusmartono Kusmartono , Suyadi Suyadi
Published: February 2017

This research was conducted to evaluate the performance of female Madura cattle in three districts of Madura Island (Sampang, Pamekasan, and Sumenep), known as breeding areas for purebred Madura cattle. Vital statistics of 1,040 female Madura cattle at 24 and 30 months old in Sampang, Pamekasan, and Sumenep district were measured and analysed. Mean of Body weight, chest girth, body length, and wither height of female Madura cattle at 24 months old were 191.7±17.6 kg, 133±3.0 cm, 124.0±2.1 cm, 115.7±2.9 cm, respectively, and those at 30 months old were 209.4±23.6 kg, 138.2±4.5 cm, 125.7±2.6 cm, 116.7±3.4 cm, respectively. The body weight, chest girth, body length of female Madura cattle at 24 and 30 months old in Pamekasan district (216.6±26.5 kg, 135.2±6.1 cm, 125.4±3.9 cm, and 238.0±28.5 kg, 141.5±6.7 cm, 127.9±3.5 cm, respectively) showed the highest value compared to those in Sampang and Sumenep district. Phenotypic correlation between vital statistics and body weight at 24 and 30 months old ranged between -0.43 to 0.90. Chest girth showed the positive highest correlation with body weight of female Madura cattle at 24 months old (0.24) and at 30 month old (0.90). It indicated that chest girth could be used as estimation parameter for body weight in female Madura cattle.
Effect of Carrot-Juice on Exopolisaccharides and β-D Galactosidase Activity in Yogurt
Authors: Radiati Lilik Eka , Jaya Firman , Oktavia Heldy
Published: February 2017

Carrot juice and milk  were  blended and fermented by  culture bacteria Streptococcus thermophillus  and Lactobacillus bulgaricus.  Carrot juice  affect significantly  on   lactic acid content   (1.09± 0.12% – 1.15± 0.01%),   pH value (3.80±0.06 – 4.17± 0.10),   viscosity  (133±2.30 cP–146±2.10cP),  β-carotene (0-173.19±1.02 µg/g), EPS (11.90 ±0.50 - 18.00 ±0.40 mg/100g),   β-D-galactosidase activity (2.27±0.30-192.40±0.48 µ/g) yogurt, but did not affect significantly on  bacteria number (9.0± 0.5 – 9.8±0.4 log CFU/g).    Carrot juice increased the yogurt culture activity with increasing acidifying, β-catotene, EPS and β-D-galactosidase, suggesting that yogurt could be fortified with carrot juice.
PEMBUATAN SELULOSA ASETAT DARI PULP KENAF (Hibiscus cannabinus)
Authors: Widyaningsih Senny , Radiman Cynthia Linaya
Published: February 2017

Cellulose acetate (CA) is one of polymer which is usually used in industry. Kenaf (Hibiscus cannabinus) pulp is one of non wood cellulose sources which can be used for producing CA. CA was produced in three step i.e. initial, acetylation, and hydrolysis. The characterization of CA kenaf had been conducted with analyzing function groups and determining relative molecule mass, acetyl content, and structure of CA. The result of infrared spectroscopy analyze showed that specific peak of acetyl group at 1237.4 cm-1 with acetyl content 40.40% and the relative molecule mass of CA was 1.15.104 with amorf structure.
PENGARUH pH LARUTAN DAN UKURAN PARTIKEL ABU SEKAM PADI TERHADAP PENURUNAN KADAR CONGO RED
Authors: Setyaningtyas Tien , Sulaeman Uyi
Published: February 2017

Rice husk used as a raw material to produce the rice husk ash. The purpose of this research were to determine the optimum contact time and maximum pH of congo red adsorption by rice husk ash, to find out the influence of particle size to adsorption The rice husk was washed, then soaked in HCl 3.84 M to remove mineral impurities, and it was dried and heated for four hours at the temperature 6000 C. The yield gained from this research is 23.44%. Optimum contact time started at after five minutes and maximum pH is six for the tree mention particle sizes 50, 100 and 140 mesh. Particle size didn’t give any significant effect to adsorption process with percent decreasing of congo red is 84.97 %, 90.39 %, 89.32 % respectively.
REAKSI TRANSESTERIFIKASI MINYAK KACANG TANAH (Arahis hypogea. L) DAN METANOL DENGAN KATALIS KOH
Authors: Purwati Purwati , Diastuti Hartiwi
Published: February 2017

Oil and fat as part of lipid are taking an important role in food industry. There are soluble vitamins, like A, D, E and K, in oil and fat. Oil, that is a source of essential acid, is a high energy source. Oil and fat take a role to improve the shape and to give a taste. This experiment have been conducted to investigate the quantity of methyl ester that is produced from transesterification reactions of peanut seed oil with methanol using KOH as a catalyst and to analyze a kind of methyl ester that is produced using GCMS.We got 68% v/v product of transesterification and we knew for kinds of methyl ester those were produced from GCMS. They are methyl palmitic, methyl linoleic, methyl stearic, dan methyl 12-hydroxil-9-octadecaenoic.
PENENTUAN KEASAMAN ZEOLIT MENGGUNAKAN METODE GRAVIMETRI, TITRASI DAN FTIR
Authors: Kartika Dwi , Kurniasih Mardiyah
Published: February 2017

Determination of natural zeolite and activated natural zeolite acidity using gravimetric, titration and FTIR methods had been carried out. The result of gravimetric method show that the acidity of the natural zeolite and activated natural zeolite was 2,350 and 5,628 mol/gram, respectively. The titration method can be obtained that the acidity degree of the natural zeolite and activated natural zeolite was 12,333 and 12,067, respectively. The result showed that the activation of the natural zeolite sample by HCl caused dealumination.
BIODEGRADASI POLIETILENA MENGGUNAKAN BAKTERI DARI TPA (TEMPAT PEMBUANGAN AKHIR) GUNUNG TUGEL KABUPATEN BANYUMAS
Authors: Zusfahair Zusfahair , Lestari Puji , Riana Ningsih Dian , Widyaningsih Senny
Published: February 2017

Plastic has high potency to become material that much threats human living in this earth, because made from chemical which cannot degraded by microbes in environment. The successful production and marketing of biodegradable plastics will help alleviate the problem of environmental pollution. One of biodegradable plastic that used in our live is polyethylene. This research reveals that local microbes capable to degrading of polyethylene. Biodegradation test was carried out by using bacteria in soil which was obtained from Gunung Tugel disposal center, Banyumas regency. Kind of polyethylene is LDPE (Low Density Polyethylene) which was obtained from Setiakawan Plastic Factory, Kalibogor, Purwokerto formed to thin film. Characterization of the polyethylene used weight loss percentage method, melting point determination and FTIR. Soil bacteria isolated from Gunung Tugel disposal center, Banyumas regency, obtained 5 single colonies, which coded GT. Bacteria isolate which have highest activity in degrading polyethylene was GT 3, with increasing the time of incubation. Weight loss percentage up to 2.33% in 1 month. Melting point of polyethylene after biodegradation was decreased that initially 210-220 °C into 210-213 °C. FTIR spectrophotometer result of polyethylene after biodegradation showed intensity for methylene and methyl cluster was decreased.
STUDI DEGRADASI ZAT PEWARNA AZO, METIL ORANYE MENGGUNAKAN FERRAT (FeO42-)
Authors: Windy Dwiasi Dian , Kurniasih Mardiyah
Published: February 2017

Synthesis of ferrate and its application to azo dyes degradation have been investigated. The synthesis was carried out by reacting Fe(NO3)3 solution with NaOCl in alkaline condition. Oxidation reaction of azo dyes was carried out by adjusting the molar ratio of ferrate to azo dyes. When ferrate reacted with azo dyes, its absorbance was monitored using UV-Vis spectrophotometer at pH condition that had been optimized. Kinetics study for azo dyes degradation was carried out at the absorbance maximum of azo dyes, as a function of time. Ferrate can oxidize azo dyes, methyl oranye effectively in optimum pH of 9.6. With the molar ratio of ferrate/azo dyes 5/1, it showed that the percentages of methyl oranye degradation reached 100%. The azo dyes degradation followed first order of kinetics. Analysis using UV-Vis spectrofotometry suggests that the products of azo dyes degradation are nitrate, ammonia, and benzene. This results showed that ferrate is an alternative and green oxidizing agent which can be used for azo dyes degradation.
UJI AKTIVITAS ANTIKANKER EKSTRAK ETANOL DAUN Rhizopora mucronata TERHADAP SEL MYELOMA
Authors: Diastuti Hartiwi , Warsinah Warsinah , Purwati Purwati
Published: February 2017

The mangrove plant have been long used the people for traditional medicine to cure various diseases, one of them to cancer therapy. Investigation the anticancer potent of R. mucronata has not been carried yet. This research was aimed to toxicity test of R. mucronta leaves extracts againts Artemia salina Leach, then cytotoxicity test of R. mucronata leave extracts againts Myeloma cancer cells. The extraction of R. mucronata leaves was peformed by maseration with ethanol. The ethanol extracts was partitioned with chloroform, ethylacetate and methanol. The ethanol extracts of R. mucronata leaves respectively was examined their toxicity againts A. salina Leach larv. The toxic exctracts was examined their citotoxicity againts Myeloma cells. The ethanol extract of R. mucronata leaves has toxic character because has LC50 < 1000 mg/mL. Citotoxicity test againts Myeloma cells showed that the chloroform fraction of ethanol extract of R. mucronata leaves have a high activity against Myeloma cell, LC50 value equal to 28,72 mg/mL. Phytochemical study showed that the active fractions contained terpenoid and alkaloid.
POTENSI HUMIN HASIL ISOLASI TANAH HUTAN DAMAR BATURRADEN DALAM MENURUNKAN KESADAHAN AIR
Authors: Setyaningtyas Tien , Andreas Roy , Riyani Kapti
Published: February 2017

The hardness water is not good for consumption because can resulted kidney disease One of the method which can be used to degrade rodamin B is adsorption use humin. Humin is biggest fraction of humat materials that insoluble in acid, alcohol and base. The ability of humin for adsorp Ca2+ and Mg2+ caused by the existence of OH phenolic and carboxylic functional group which can interacted with metal ion. The aim of this study is to recognize humin characteristic from the soil of Baturraden resin forest, and ability of humin from the soil of Baturraden resin forest for decreasing the hardness water.Humin was isolated from the Baturraden resin forest and purified use mixture of HCl:HF. Purified humin is characterised such as identify of functional group of humin, stipulating of water content, stipulating of dust content, obstetrical stipulating of total acidity content, carboxylic and OH phenolic functional group. Decreasing of hardness water was analysed with variation time 0, 10, 30, 60, 180, 300, 600, 900, 1200 dan 1440 minute.Humin that isolated from Baturraden resin forest have characteristic such as water content 16.6199 %; dust content 9.2050 %; total acidity content 475 cmol / Kg; carboxylic rate 200 cmol/Kg, and OH phenolic rate 275 cmol/Kg. Decreasing of hardness water in Darmakradenan, Ajibarang subdistrict, Banyumas regency was 54,745 % with equlibrium time at 600 minute (10 hours).
ANALISIS SENYAWA KIMIA DALAM EKSTRAK KLOROFOM BUNGA KAMBOJA (Plumeria alba) DENGAN GC-MS
Authors: Nur Handayani Santi , Riyani Kapti
Published: February 2017

lumeria alba is called frangipani is one of Apocynaceae family’s plant. Frangipani is potentially used as traditional medicine, for example as an antiinflamation drugs. It has not been yet done an extensive reasearch on the chemical compound content of frangipani. The reasearch was conducted to investigate some chemical constituents by using Gas Chromatography-Mass Spectrometer (GC-MS). Extraction of chemical compound of frangipani has been conducted by soxhletasi using n-hexane as solvent followed by chloroform and ethyl acetate. Then chloroform extract was identified by GC-MS. The result showed that extract of chloroform contained : palmitic acid, clionasterol, fucosterol, fagarasterol and methyl commate B respectively.
DEKOLORISASI LIMBAH BATIK TULIS MENGGUNAKAN JAMUR INDIGENOUS HASIL ISOLASI PADA KONSENTRASI LIMBAH YANG BERBEDA
Authors: Stia Dewi Ratna , Lestari Sri
Published: February 2017

Azo as batik dyes are textile dyes which difficult to degradate. Fungus as bioremidiation organism are choosed to decolorize the dyes because its transformation ability, it can degradate toxic dyes component. The aim of research are to explore the fungus from Sokaraja-Banyumas batik industrial dyestuff, to know potential indigenous species wich can degradate it, to know dyestuff consentration which is degradated.Result of research showed that the isolation process of indigenous fungi from batik dyestuff in District Sokaraja Banyumas produce 4 isolates that have the potential dekolorization, they are 3 isolates of the genus Fusarium, and 1 isolate of the genus Aspergillus. That indigenous fungus can be used to decolorize dyestuff batik the decolorize percentage 69.346% -82.421%.
SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL
Authors: Indrasanti Diana , Haryanto Aris , T. Artama Wayan
Published: February 2017

Microneme protein (MIC) is one of proteins that belongs to excretory-secretory antigens (ESAs) of Toxoplasma gondii. Microneme 3 protein (MIC-3) is the protein that plays an important role in the invasion proccess during cell infection as a mediator attachment parasite to the host cell. The aim of this research is to clone mic3 (gene encoding for MIC-3) of T. gondii from local isolate using recombinant DNA technology by cloning mic3 in an expression vector. Deoxyribonucleic acid (DNA) from T. gondii tachyzoites was amplified by PuRe Taq RTG-PCR Beads using mic3 specific primers. Amplified DNA was double digested using EcoRV and HindIII restriction endonucleases and then purified using EZ-10 spin coloumn purification kit. The mic3 DNA was ligated into pET-32a(+) expression vector and transformated into Escherichia coli BL21. The results showed that recombinant mic3gene 4.2 kDa has been successfully performed by cloning gene encoding for MIC-3 protein of T. gondii local isolate into pET-32a(+) and transformed to E. coli BL21.
SINTESIS DAN KARAKTERISASI CROSSLINK KITOSAN DENGAN TRIPOLIFOSFAT pH 3
Authors: Kurniasih Mardiyah , Hidayat Aprilita Nurul , Kartini Indriana
Published: February 2017

Chitosan is polycationic in acidic media and can interact with negatively charged species such as tripolyphosphate (TPP), which is a nontoxic crosslinking agent. The synthesis of TPP crosslinked chitosan was perfomed by reacting TPP solution (pH 3) with chitosan solution. The aim of this study was to prepared and characterize the crosslinked chitosan-tripolyphosphate samples. Characterization included determination of water and ash degree, moreover crosslinked chitosan-tripolyphosphate powder characterize with FTIR and XRD spectroscopy. The result showed that process efficiency of crosslinked chitosan-tripolyphosphate was 88.49%, with degree of water and ash were 25.70 and 16.72%, respectively.
EFEKTIVITAS ECENG GONDOK (Echhornia crassipes) DALAM PENYERAPAN KADMIUM (Cd) PADA LEACHATE TPA GUNUNG TUGEL
Authors: Lestari Sri , Santoso Slamet , Anggorowati Sulastri
Published: February 2017

Leachate is liquid of result organic garbage that contain substance is dissolved and suspension as decomposition by microorganism. Leachate contain material organic with high level and many formed at landfill with open dumping system. Material organic in leachate can be reduced by phytoremediation with water hyacinth (Echhorniacrassipes). The aim of research are know large closure of water hyacinth, long residence time and the best interaction to reduce the levels of material organic in leachate. Method of research was designed experimental based on Completely Randomized Design (CRD) with Factorial Design. The first factor are closing large percentage of water hyacinth (0%, 25%, 50%, 75% and 100%) and the second factor are long residence time (4 days, 5 days and 6 days). Result of research showed that is closing large of 75% is the best treatment to reducing level of Cd that is 29,279%. and long residence time of 6 days is the best treatment to reducing level of Cd that is 27,211%. Interaction closing large of 75% and long residence time of 6 days is the best treatment to reducing level of Cd that is 39,770%
STUDI PENURUNAN ZAT WARNA ACID ORANGE 7 DENGAN PROSES OKSIDASI MENGGUNAKAN FERRAT (FeO4)2-
Authors: Windy Dwiasi Dian , Suyata Suyata
Published: February 2017

Colour removal from textile wastewater has been a matter of considerable interest during the last two decades, not only because of the potential toxicity of certain dyes but often due to their visibility in receiving waters and to their low biodegradability. Due to the limited success of some physical and chemical techniques for the treatment of dye effluents it is necessary to develop destructive systems leading to complete mineralization or, at least, to less harmful or easy-to-treat compounds. Oxidation processes using ferrate (FeO4)2- have been found to be very effective in the degradation of dye pollutants and for the treatment of waste waters from the textile industry.Acid Orange 7 (AO7) commonly used as a textile dye and could be degraded by ferrate oxidation processes. In the oxidation degradation of dye by ferrate process, effect of some parameters such as time, pH, and molar ratio of dye was examined at experimental condition. AO7 removal by this process was calculated to be equal to 12 minute at experimental condition. Ferrate can oxidize acid orange 7 effectively at optimum pH of 9.8, with the molar ratio of ferrate : acid orange 7 at 4:1. The percentages of acid orange 7 degradation reached to 98.9%.
KARAKTERISASI SENYAWA POLIOKSOMETALAT TIPE KEGGIN TERSUBSTITUSI VANADIUM MENGGUNAKAN FT-IR DAN 51V NMR
Authors: Lesbani Aldes
Published: February 2017

Sintesis dan karakterisasi senyawa polioksometalat tipe Keggin yakni Rb2K2[γ-SiV2W10O38(OH)2]•nH2O dan H4[γ-SiV2W10O38(OH)2]•nH2O telah dilakukan menggunakan FT-IR dan spektroskopi 51V NMR. Senyawa polioksometalat Rb2K2[γ-SiV2W10O38(OH)2]•nH2O ditransformasi menjadi H4[γ-SiV2W10O38(OH)2]•nH2O melalui metode pertukaran ion menggunakan asam klorida. Spektrum 51V NMR dari Rb2K2[γ-SiV2W10O38(OH)2]•nH2O menunjukkan pergeseran kimia pada -586.94 ppm. Vibrasi utama yang menunjukkan gugus fungsional senyawa Rb2K2[γ-SiV2W10O38(OH)2]•nH2O muncul pada bilangan gelombang 1041 cm-1, 972 cm-1 (n W=O), 914 cm-1 (n Si-O), 870 cm-1 (nW-Oe-W), 781 cm-1 (n W-Oc-W), 565 cm-1 dan 534 cm-1. Pergeseran kimia 51V NMR dari senyawa polioksometalat H4[γ-SiV2W10O38(OH)2]•nH2O memberikan satu puncak pada -586.77 ppm. Pergeseran kimia antara senyawa Rb2K2[γ-SiV2W10O38(OH)2]•nH2O dan H4[γ-SiV2W10O38(OH)2]•nH2O tidak berubah yang disebabkan oleh kemiripan struktur utama di antara dua senyawa polioksometalat tersebut. Vibrasi utama dari senyawa polioksometalat H4[γ-SiV2W10O38(OH)2]•nH2O juga mempunyai kemiripan dengan senyawa polioksometalat Rb2K2[γ-SiV2W10O38(OH)2]•nH2O yang disebabkan hanya kation yang berubah menjadi asam.
IDENTIFIKASI SENYAWA DAN AKTIVITAS ANTIMALARIA IN VIVO EKSTRAK ETIL ASETAT TANAMAN ANTING-ANTING (Acalypha indica L.)
Authors: Kamilah Hayati Elok , Jannah Akyunul , Ningsih Rachmawati
Published: February 2017

Telah dilakukan penelitian identifikasi ekstrak etil asetat dari tanama Anting-anting (Acalypha indica Linn.) sebagai senyawa antimalaria dan aktivitasnya secara in vivo pada sel parasit malaria P. berghei.Penelitian ini meliputi ekstraksi tanaman anting-anting menggunakan metode ekstraksi maserasi selama 24 jam dengan variasi pelarut yaitu etil asetat, diklorometana, dan petroleum eter. Pengadukkan dibantu dengan shaker selama 3 jam. Ekstrak pekat diuji fitokimia didukung Kromatografi Lapis Tipis, Ekstrak pekat etil asetat dilakukan uji antimalaria in vivo terhadap hewan uji terhadap sel parasit P. berghei. Data derajat parasitemia mencit dianalisis menggunakan program SPSS dengan Uji OneWay ANOVA dan dilanjutkan dengan Uji Tukey.Hasil penelitian menunjukkan adanya senyawa aktif tanin, alkaloid dan steroid pada ekstrak etil asetat.Uji aktivitas antimalaria secara in vivo pada hewan coba didapatkan hasil penghambatan ekstrak etilasetat terhadap pertumbuhan Plasmodium berghei pada dosis 0,01 mg/g bb sebesar 87,19%; pada dosis 0,1mg/g bb sebesar 84,9% dan pada dosis 1mg/g bb sebesar 90,74%.
PEMBUATAN DAN UJI KEMAMPUAN MEMBRAN KITOSAN SEBAGAI MEMBRAN ULTRAFILTRASI UNTUK PEMISAHAN ZAT WARNA RHODAMIN B
Authors: Kusumawati Nita , Tania Septiana
Published: February 2017

Telah dilakukan penelitian tentang pembuatan membran kitosan dan aplikasinya dalam pemisahan zat warna Rhodamin B. Tujuan penelitian ini adalah untuk mengetahui karakteristik fisik serta kinerja membran kitosan yang dihasilkan dalam memisahkan zat warna Rhodamin B dengan variasi konsentrasi kitosan dan tekanan operasional. Penelitian ini diawali dengan pembuatan membran dengan variasi konsentrasi kitosan, yaitu 1-5%. Membran kitosan dibuat dengan mencampurkan kitosan dalam asam asetat 1% kemudian dicetak pada cawan petri. Uji karakteristik fisik membran meliputi, kekuatan tarik dan regangan membran menggunakan Autograph, dan morfologi serta ukuran pori membran menggunakan Scanning Eletron Microscopy (SEM). Kinerja membran meliputi nilai fluks dan rejeksi, diketahui dari pengukuran menggunakan alat uji membran “dead-end” dan UV-Visible Genesys 10. Hasil penelitian menunjukkan bahwa membran kitosan yang dihasilkan masuk dalam rentang membran ultrafiltrasi. Membran memiliki daya tegang dan regang yang tinggi dengan bertambahnya konsentrasi kitosan. Nilai tegangan (Load) yang diperoleh sebesar 0,1531–2,8571 kgf dan nilai regangan (Stroke) sebesar 2,86-5,48%. Semakin besar konsentrasi kitosan dalam membran akan menghasilkan fluks yang kecil. Nilai fluks terbaik dihasilkan oleh membran kitosan 1% pada tekanan 5 kg/cm2 yakni sebesar 38,372 L/m2 jam. Sedangkan nilai koefisien rejeksi tertinggi dihasilkan oleh membran kitosan 3% pada tekanan 1 kg/cm2 yakni sebesar 88,27%.
DEGRADASI ZAT WARNA KONGO MERAH LIMBAH CAIR INDUSTRI TEKSTIL DI KABUPATEN PEKALONGAN MENGGUNAKAN METODE ELEKTRODEKOLORISASI
Authors: Suyata Suyata , Kurniasih Mardiyah
Published: February 2017

Degradasi zat warna kongo merah limbah cair industri tekstil menggunakan metode elektrodekolorisasi telah dilakukan. Eksperimen dilakukan dengan mengelektrolisis kongo merah menggunakan katoda karbon dan anoda besi. Analisis konsentrasi kongo merah dilakukan menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa dibawah kondisi optimum pada voltase 12 V- jarak elektroda 1 cm-pH 4- konsentrasi H2O2500 mg/L selama 12 menit, zat warna kongo red limbah cair industri tekstil dapat didegradasi 99,83 %. Metode ini dapat digunakan untuk mendegradasi zat warna kongo merah.
SEBARAN KANDUNGAN LOGAM BERAT Cd PADA SEDIMEN DI MUARA SUNGAI WAY KUALA BANDAR LAMPUNG
Authors: Hidayat Diky , Putu Inda Novita Ni
Published: February 2017

Penelitian penentuan penyebaran konsentrasi logam berat Cd pada sedimen di muara sungai Way Kuala telah dilakukan. Tujuan penelitian ini adalah untuk melihat penyebaran konsentrasi logam berat pada sedimen di muara tersebut. Konsentrasi Cd ditentukan dengan menggunakan spektrofotometer serapan atom (SSA), dengan menggunakan empat validasi metode yaitu batas deteksi, presisi, akurasi, dan linieritas. Hasil menunjukkan bahwa konsentrasi logam berat Cd dalam sampel sedimen di hulu, badan, dan hilir dari muara sungai Way Kuala berada dalam kisaran 20.73 ± 0.18 sampai 23.00 ± 0.81 ppm diatas kualitas standar sedimen (0.65 sampai 2.49 ppm) yang telah ditetapkan oleh National Sediment Quality Survey USEPA pada tahun 2004. Validasi metode pada penentuan logam berat Cd dalam sedimen menunjukkan presisi  dengan nilai simpangan baku relatif lebih kecil dari 5% (RSD <5%); akurasi 80-120%; batas deteksi dan koefisien korelasi pada logam Cd sebesar 0.01 dan 0.998.
ISOLASI, IDENTIFIKASI KOMPONEN DAN UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI RIMPANG LEMPUYANG WANGI (Zingiber aromaticum Val.)
Authors: Handayani Nestri , Wartono Widyo , Wijaya Nirub
Published: February 2017

Isolasi, identifikasi dan uji aktivitas antibakteri minyak atsiri rimpang lempuyang wangi (Zingiber aromaticum Val.) telah dilakukan. Minyak atsiri diisolasi dengan metode destilasi Stahl dan dianalisis dengan GC-MS. Kadar minyak atsiri yang dihasilkan 0,6% (v/b). Identifikasi komponen dilakukan dengan membandingkan spektrum massa masing-masing senyawa dengan spektrum massa senyawa standar dari literatur Wiley 7. LIB. Hasil analisis menunjukkan 27 senyawa teridentifikasi yaitu α – pinen (0.36%), Z – β ocimen (2.70%), Champhen (10.91%), Sabinen (0.07%), β – pinen (0.18%), β – myrcen (0.29%),(Z)-α-osimen (0.51)%, allo-osimen (0.08)%, p – cimen (0.50%), 1,8 cineol (2.75%), β – trans ocimen (0.05%), γ – terpinen (0.14%), α – terpinolen (27.19%),  champor (2.71%), Isobornyl alcohol (1.51%), Terpinen 4-ol (1.11%), 3-cycloheksan 1 metanol (0.25%), Isobornyl acetat (0.29%), Trans caryophilen (0.47%), α-humulen (7.53%), α-kurkumen (0.13)%, farnesol (0.11%), (-)-kariofilen oksida (2.07%), patchulana (0.25%), Zerumbon (31.05%).Uji aktivitas antibakteri minyak atsiri rimpang lempuyang wangi (Zingiber aromaticum Val.) telah dilakukan terhadap Pseudomonas aeruginosa dan Salmonella typhi dengan metode difusi agar menggunakan metode difusi dengan membuat sumuran. Hasil uji antibakteri menunjukkan bahwa minyak atsiri rimpang lempuyang wangi (Zingiber aromaticum Val) memiliki aktivitas antibakteri terhadap semua bakteri uji dengan Konsentrasi Hambat Minimum (KHM) 0,25% untuk Pseudomonas aeruginosa,  dan 0,075%  untuk Salmonella typhi. Dibandingkan dengan amoksisilin, potensi antibakteri minyak atsiri rimpang lempuyang wangi (Zingiber aromaticum Val) pada kedua bakteri uji adalah 0,001% dari potensi amoksisilin.
HUBUNGAN KUANTITATIF STRUKTUR-AKTIVITAS (HKSA) ANTIKANKER SENYAWA TURUNAN KALANON DENGAN METODE SEMI EMPIRIS PM3 (PARAMETERIZED MODEL 3)
Authors: Vaulina Y.D. Eva , Chasani Mochammad , Abdulghani Mokhammad
Published: February 2017

Kalanon adalah senyawa turunan kumarin yang diisolasi dari spesies Calophyllum teysmannii yang tumbuh di hutan tropis Kalimantan dan Malaysia. Kalanon dan beberapa senyawa turunannya mempunyai potensi sebagai antikanker, namun aktivitasnya masih relatif rendah dengan senyawa paling aktif yaitu benzoilglisin ester kalanonyang mempunyai IC50 36,48 µg/mL terhadap sel Leukimia L1210. Oleh karena itu perlu dilakukan modifikasi molekul kalanon untuk mendapatkan senyawa turunan kalanon baru yang mempunyai aktivitas lebih baik. Modifikasi molekul suatu senyawa dapat dilakukan melalui kajian HKSA untuk mengurangi resiko trial and error di laboratorium. Penelitian ini berdasarkan metode perhitungan semi empiris PM3 melalui pendekatanMultiple Linear Regression (MLR) dengan log 1/IC50 sebagai variabel tergantung dan variabel bebasnya berupa 31 muatan bersih atom, momen dwikutub, selisih enegi orbital HOMO-LUMO (ΔΕ), koefisien partisi oktanol-air (log P),indeks Harary dan indeks Randic. Persamaan HKSA yang dihasilkan dari penelitian ini adalah log1/IC50= -1,194-0,029logP + 0,002Harary + 0,403ΔE + 53,983qC12 + 0,439qC19 dengan n= 11, r= 0,958, r2= 0,917, SE= 0,0561773, Fhitung/Ftabel = 2,19960, dan PRESS = 0,097115. Berdasarkan persamaan HKSA tersebut, didapatkan empat senyawa prediksi dengan aktivitas teoritik lebih baik dibandingkan senyawa turunan kalanon sebelumnya, yaitu senyawa prediksi 12, 22, 24, dan 25 yang masing-masing mempunyai IC50 teoritik 26,44; 25,609; 27,050; dan 23,013 µg/mL terhadap sel Leukimia L1210.
DEGRADASI ZAT WARNA AZO TARTRAZIN PADA LIMBAH CAIR MIE DENGAN METODE AOPs (Advanced Oxidation Processes)
Authors: Setyaningtyas Tien , Windy Dwiasi Dian
Published: February 2017

Penggunaan metode untuk mendegradasi zat warna dari limbah industri dengan proses AOPs (Advanced Oxidation Processes) atau proses oksidasi lanjut saat ini banyak dikembangkan terutama untuk limbah cair zat warna yang sulit terdegradasi. Salah satu industri makanan yang menggunakan bahan aditif zat warna pada proses produksinya adalah industri mie yaitu menggunakan zat warna tartrazin. Dampak negatif perkembangan industri mie adalah timbulnya pencemaran lingkungan dari limbah cair industri mie yang masih mengandung zat warna tartrazin. Penurunan kadar zat warna tartrazin dalam limbah cair mie dapat diupayakan dengan cara mendegradasi zat warna tartrazin melalui metode AOPs.Proses AOPs untuk mendegradasi tartrazin dalam limbah cair mie pada penelitan ini menggunakan kombinasi H2O2dengan sinar matahari sebagai sumber sinar UV (H2O2/UV-Vis). Paramaeter yang dilakukan antara lain variasi konsentrasi H2O2 yaitu 0,01 M ; 0,02 M dan 0,03 M dengan variasi waktu kontak 0; 0,5 ; 1 ; 1,5 ; 2 ; 2,5 ; 3 ; 3,5 ; 4 jam. Pengaruh pH terhadap degradasi tartrazin dengan variasi 2, 5, 7, 9 dan 12.Hasil yang diperoleh bahwa degradasi tartrazin tidak dipengaruhi oleh konsentrasi H2O2 tetapi konsentrasi H2O2mempengaruhi waktu kesetimbangan. Waktu kesetimbangan paling cepat dicapai 30 menit pada konsentrasi H2O20,03 M, sedangkan untuk H2O2 0,01M dan 0,02 M waktu kesetimbangan tercapai pada 2,5 jam dengan penurunan tartrazin sebesar 45%. Proses AOPs pada degradasi tartrazin dipengaruhi oleh harga pH. Pada pH rendah penurunan tartrazin semakin besar. Pernurunan tartrazin paling besar pada pH 2 yaitu sebesar 65%.
AKTIVITAS ANTIBAKTERI Eschericia coli PADA MINYAK ATSIRI BATANG GENOAK (Acorus calamus) ASAL PULAU TIMOR
Authors: M. F. Ledoh Sherlly , I. Lerrick Reiner , Ratu Debora
Published: February 2017

Telah dilakukan uji aktivitas antibakteri Eschericia coli (E. coli) minyak batang genoak (Acorus calamus). Penelitian ini dilakukan melalui destilasi uap batang genoak yang diikuti analisis komposisi kimianya menggunakan GC-MS, dan uji antibakteri terhadap bakteri E. coli menggunakan metode hitung cawan. Hasil penelitian diperoleh minyak genoak dengan rendemen 0,17% dan asaron sebagai komponen utama minyak genoak sebesar 89,81%. Minyak genoak dapat menghambat pertumbuhan bakteri E. coli sebesar 95,76% pada kosentrasi 10% (v/v).
STUDI KINETIKA REAKSI ESTERIFIKASI ENZIMATIS ASAM MIRISTAT DENGAN OLEIL ALKOHOL
Authors: Yuanita Emmy , Ryantin Gunawan Erin , Kurniawati Lely , Raudhatul Kamali Siti
Published: February 2017

Studi kinetika reaksi esterifikasi enzimatisasam miristat dan oleil alkohol telah dilakukan. Penelitian ini bertujuan untuk mendapatkan hasil sintesis yang optimal, sehingga meningkatkan konversi dan kecepatan reaksi pembentukan wax ester. Analisis kualitatif wax ester yang terbentuk dilakukan secara TLC dan GC, sedangkan analisis secara kuantitatif dilakukan secara titrasi asam-basa. Dari hasil penelitian diperoleh % konversi wax esterdengan menggunakan enzim tanpa perlakuan sebesar 94,44%. Nilai Km dan Vmax untuk asam miristat sebesar 2,5649 dan 14,3823 mmol x10-5 mmol/menit.mg katalis sedangkan untuk oleil alkohol sebesar 7,0345 dan 29,2292 mmol x10-mmol/menit.mg katalis. Sehingga dapat disimpulkan bahwa kinetika reaksi esterifikasi asam miristat dengan oleil Alkohol yang dikatalisis oleh enzim lipase tanpa perlakuan mengikuti Model Michaelis-Menten sedangkan mekanisme reaksi esterifikasi mengikuti mekanisme Bi Bi Ping-Pong
FOTODEGRADASI SIANIDA DALAM LIMBAH CAIR TAPIOKA
Authors: Riyani Kapti , Setyaningtyas Tien
Published: February 2017

Sianida banyak terdapat pada limbah cair tapioka. Sianida dapat didegradasi menggunakan fotokatalis TiO2 dengan aktivasi sinar UV, sinar UV dapat berasal dari lampu UV dan sinar matahari.Tujuan dari penelitian ini adalah mendegradasi sianida menggunakan fotokatalis TiO2 menggunakan sumber sinar lampu UV dan sinar matahari.Fotokatalis TiO2 yang digunakan dalam penelitian ini dibuat lapis tipis pada plat kaca dengan ukuran 7 x 10 cm2. Variasi sumber sinar yaitu lampu UV dan sinar matahari dipelajari pada pH 9.Hasil penelitian menunjukkan bahwa penurunan kadar sianida menggunakan sumber sinar lampu UV dengan lama penyinaran 8 jam sebesar 90,69 %, sedangkan penurunan kadar sianida menggunakan sumber sinar matahari dengan lama penyinaran 8 jam sebesar 98,74 %.
SESKUITERPEN FURANODIENON DARI RIMPANG Curcuma xanthorrhiza DAN AKTIVITAS ANTIBAKTERINYA
Authors: Diastuti Hartiwi , Maolana Syah Yana , Dewi Juliawaty Lia , Singgih Marlia
Published: February 2017

ABSTRAK [Full Text]Curcuma xanthorrhiza dikenal sebagai tanaman obat Indonesia, rimpangnya sering digunakan dalam pengobatan tradisional. Penelitian ini bertujuan untuk mengisolasi senyawa seskuiterpen dari rimpang Curcuma xanthorrhizadan menguji aktivitas antibakterinya. Isolasi senyawa dilakukan dengan kromatografi kolom vakum cair dan kromatografi radial. Identifikasi struktur dilakukan secara spektroskopi (FTIR (Fourier Transfrom Infra Red), NMR (Nuclear Magnetic Resonance) 1D dan 2D, serta MS (Mass Spectroscopy)). Uji antibakteri dilakukan dengan metode mikrodilusi, terhadap beberapa bakteri uji yaitu Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis  dan Staphylococcus aureus,. Isolat yang diperoleh berupa minyak tak berwarna yang teridentifikasi sebagai senyawa seskuiterpen furanodienon. Furanodienon menunjukkan aktivitas antibakteri dengan  nilai  MIC dan MBC 125,0 dan 250,0 µg/mL .
UJI POTENSI ANTIPLASMODIUM EKSTRAK BUAH PARE (Momordica charantia L.) TERHADAP Plasmodium falcifarum
Authors: Susilawati Susilawati , Hermansyah Hermansyah
Published: February 2017

Malaria masih menjadi salah satu penyakit endemis dan masalah kesehatan utama di Indonesia.  Buah pare (Momordica charantia L.) secara tradisional sering digunakan sebagai obat.   Tujuan dari penelitian ini adalah untuk menguji potensi antiplasmodium in vitro ekstrak buah pare terhadap Palsmodium falciparum sehingga dapat digunakan sebagai obat anti malaria.   Buah pare diekstrak dengan metode maserasi menggunakan pelarut metanol.  Uji aktivitas antiplasmodium dilakukan secara pengamatan mikroskopik pada kultur strain P. falcifarum  3D7. Berdasarkan hasil pengujian, sampel ekstrak metanol buah pare memiliki aktivitas penghambatan terhadap pertumbuhan parasit P. falciparum 3D7. Kekuatan aktivitas antimalaria dengan nilai IC50 = 0,39 µg/mL.
KARAKTERISASI KATALIS Pt-Pd/ZEOLIT ALAM REGENERASI PADA REAKSI HIDRODENITROGENASI PIRIDIN
Authors: Asnawati Dina
Published: February 2017

Telah dilakukan penelitian tentang Karakterisasi Katalis Pt-Pd/Zeolit Alam Regenerasi pada Reaksi Hidrodenitrogenasi Piridin. Tujuan dari penelitian ini adalah untuk mempelajari karakter katalis Pt-Pd/zeolit alam baru, bekas dan hasil regenerasi pada hidrodenitrogenisasi piridin. Katalis Pt-Pd/zeolit alam terdeaktivasi diregenerasi dengan cara dioksidasi dengan gas O2 pada temperatur 350oC selama 2 jam, dan direduksi dengan gas H2 pada temperatur 400oC selama 1 jam. Karakterisasi katalis baru (fresh), terdeaktivasi dan terregenerasi meliputi penentuan luas permukaan, volume pori dan rerata jejari pori dengan alat Gas Sorption Analyzer NOVA-1000 berdasarkan adsorpsi gas N2 serta penentuan keasaman dengan metode adsorpsi gas amoniak. Hasil penelitian menunjukkan bahwa deaktivasi katalis menyebabkan penurunan luas permukaan spesifik, volume total pori dan keasaman katalis, sedangkan proses regenerasi pada katalis bekas dengan metode oksidasi dan reduksi meningkatkan luas permukaan spesifik, volume total pori, rerata jejari pori dan keasaman katalis.
FOTODEGRADASI ZAT WARNA TARTRAZIN LIMBAH CAIR INDUSTRI MIE MENGGUNAKAN FOTOKATALIS TiO2 - SINAR MATAHARI
Authors: Windy Dwiasi Dian , Setyaningtyas Tien
Published: February 2017

Salah satu dampak negatif perkembangan industri mie di Indonesia adalah timbulnya pencemaran lingkungan dari limbah cair industri mie yang masih mengandung zat organik seperti zat warna tartrazin. Penurunan kadar zat warna tartrazin dalam limbah cair mie dapat diupayakan dengan cara mendegradasi zat warna tartrazin melalui proses fotodegradasi dengan metode fotokatalis. Pada penelitian ini telah dilakukan fotodegradasi zat warna tartrazin dalam limbah cair mie menggunakan fotokatalis TiO2. Perlakuan meliputi pengaruh variasi waktu kontak dan pH terhadap aktivitas fotokatalis TiO2 menggunakan sinar UV dan sinar matahari. Hasil penelitian menunjukkan bahwa aktivitas fotokatalitik yang terbaik untuk  TiO2 adalah pada kondisi limbah cair mie dengan pH 2, dan waktu kontak 1 jam. Persentase penurunan kadar tartrazin dengan menggunakan lampu UV adalah sebesar 56,81%, sedangkan dengan penyinaran sinar matahari sebesar 61,64 %.