bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence and characteristics of ESBL-producing Escherichia coli in clinically healthy pigs: implications for antibiotic resistance spread in livestock
Authors: Oliveira Rúzivia Pimentel , da Silva Juliana Soares , da Silva Giarlã Cunha , Rosa Jéssica Nogueira , Bazzolli Denise Mara Soares , Mantovani Hilario C
Published: March 2024

Aim

This study aimed to compare and characterize the resistance profile and the presence of extended-spectrum beta-lactamase (ESBL) related genes in Escherichia coli isolated from healthy finishing pigs fed with or without antibiotics in their diets.

Methods and results

A total of 27 ceftiofur-resistant E. coli isolates were obtained from 96 healthy pigs. The antibiotic resistance profile was tested, and all 27 isolates were classified as multidrug-resistant (MDR). A high proportion of isolates were resistant to cephalosporins, ampicillin, ciprofloxacin, and tetracyclines. The ESBL production was observed in 85% of isolates by double-disc synergy test. The MDR-E. coli isolates harbored ESBL genes, such as blaTEM, blaCTX-M-1, blaCTX-M-2, and blaCTX-M-8,25. In addition, other antibiotics resistance genes (ARGs) were also detected, such as sul2, ant(3″)-I, tetA, and mcr-1. The mobilization of the blaCTX-M gene was confirmed for nine E. coli isolates by conjugation assays. The presence of blaCTX-M on mobile genetic elements in these isolates was demonstrated by Southern blot hybridization, and the resistance to cephalosporins was confirmed in the transconjugants. Our results indicate the prevalence of CTX-M-producing E. coli strains harboring mobile genetic elements in the normal microbiota of healthy pigs.

Conclusions

These findings highlight the significance of ESBL genes as a global health concern in livestock and the potential spread of antimicrobial resistance to other members of the gastrointestinal tract microbiota.
Skeletal fluorosis secondary to methoxyflurane use for chronic pain
Authors: Park Yeung-Ae , Plehwe Walter E , Varatharajah Kapilan , Hale Sophie , Christie Michael , Yates Christopher J
Published: March 2024

Skeletal fluorosis is rare and occurs secondary to chronic high amounts of fluoride consumption, manifesting as diffuse osteosclerosis, skeletal pain, connective tissue calcification, and increased fracture risk. Methoxyflurane is a volatile, fluorinated hydrocarbon-inhaled analgesic, and the maximum recommended dose is 15 mL (99.9 % w/w) per wk. A rodent study found increased skeletal fluoride after methoxyflurane exposure. However, skeletal fluorosis secondary to methoxyflurane use in humans has rarely been reported. We present the case of a 47-yr-old female with diffuse osteosclerosis secondary to fluorosis from methoxyflurane use for chronic pain, presenting with 3 yr of generalized bony pain and multiple fragility fractures. Lumbar spine BMD was elevated. CT and radiographs demonstrated new-onset marked diffuse osteosclerosis, with calcification of interosseous membranes and ligaments, and a bone scan demonstrated a grossly increased uptake throughout the skeleton. Biochemistry revealed an elevated alkaline phosphatase and bone turnover markers, mild secondary hyperparathyroidism with vitamin D deficiency, and mild renal impairment. Zoledronic acid, prescribed for presumed Paget’s disease, severely exacerbated bony pain. Urinary fluoride was elevated (7.3 mg/L; reference range < 3.0 mg/L) and the patient revealed using methoxyflurane 9 mL per wk for 8 yr for chronic pain. A decalcified bone biopsy revealed haphazardly arranged cement lines and osteocytes lacunae and canaliculi, which was consistent with an osteosclerotic process. Focal subtle basophilic stippling around osteocyte lacunae was suggestive of fluorosis. Although fluorosis is not a histological diagnosis, the presence of compatible histology features was supportive of the diagnosis in this case with clinical–radiological–pathological correlation. Skeletal fluorosis should be considered as a cause of acquired diffuse osteosclerosis. Methoxyflurane should not be recommended for chronic pain. The risk of repeated low-dose exposure to fluoride from methoxyflurane use as analgesia may be greater than expected, and the maximum recommended dose for methoxyflurane may require re-evaluation to minimize skeletal complications.

Abbreviated abstract

Skeletal fluorosis is rare and occurs secondary to chronic high amounts of fluoride consumption, manifesting as diffuse osteosclerosis, skeletal pain, connective tissue calcification, and increased fracture risk. We present the case of a 47-yr-old female with skeletal fluorosis secondary to long-term methoxyflurane for chronic pain. The risk of repeated low-dose exposure to fluoride from methoxyflurane use for analgesia may be greater than expected, and the maximum recommended dose for methoxyflurane may require re-evaluation to minimize skeletal complications.
Germline ecology: Managed herds, tolerated flocks, and pest control
Authors: Haig David
Published: March 2024

Multicopy sequences evolve adaptations for increasing their copy number within nuclei. The activities of multicopy sequences under constraints imposed by cellular and organismal selection result in a rich intranuclear ecology in germline cells. Mitochondrial and ribosomal DNA are managed as domestic herds subject to selective breeding by the genes of the single-copy genome. Transposable elements lead a peripatetic existence in which they must continually move to new sites to keep ahead of inactivating mutations at old sites and undergo exponential outbreaks when the production of new copies exceeds the rate of inactivation of old copies. Centromeres become populated by repeats that do little harm. Organisms with late sequestration of germ cells tend to evolve more “junk” in their genomes than organisms with early sequestration of germ cells.
Spatial intratumor heterogeneity of programmed death-ligand 1 expression predicts poor prognosis in resected non–small cell lung cancer
Authors: Nagasaki Yusuke , Taki Tetsuro , Nomura Kotaro , Tane Kenta , Miyoshi Tomohiro , Samejima Joji , Aokage Keiju , Ohtani-Kim Seiyu Jeong-Yoo , Kojima Motohiro , Sakashita Shingo , Sakamoto Naoya , Ishikawa Shumpei , Suzuki Kenji , Tsuboi Masahiro , Ishii Genichiro
Published: March 2024

Background

We quantified the pathological spatial intratumor heterogeneity of programmed death-ligand 1 (PD-L1) expression and investigated its relevance to patient outcomes in surgically resected non–small cell lung carcinoma (NSCLC).

Methods

This study enrolled 239 consecutive surgically resected NSCLC specimens of pathological stage IIA-IIIB. To characterize the spatial intratumor heterogeneity of PD-L1 expression in NSCLC tissues, we developed a mathematical model based on texture image analysis and determined the spatial heterogeneity index of PD-L1 for each tumor. The correlation between the spatial heterogeneity index of PD-L1 values and clinicopathological characteristics, including prognosis, was analyzed. Furthermore, an independent cohort of 70 cases was analyzed for model validation.

Results

Clinicopathological analysis showed correlations between high spatial heterogeneity index of PD-L1 values and histological subtype (squamous cell carcinoma; P < .001) and vascular invasion (P = .004). Survival analysis revealed that patients with high spatial heterogeneity index of PD-L1 values presented a significantly worse recurrence-free rate than those with low spatial heterogeneity index of PD-L1 values (5-year recurrence-free survival [RFS] = 26.3% vs 47.1%, P < .005). The impact of spatial heterogeneity index of PD-L1 on cancer survival rates was verified through validation in an independent cohort. Additionally, high spatial heterogeneity index of PD-L1 values were associated with tumor recurrence in squamous cell carcinoma (5-year RFS = 29.2% vs 52.8%, P < .05) and adenocarcinoma (5-year RFS = 19.6% vs 43.0%, P < .01). Moreover, we demonstrated that a high spatial heterogeneity index of PD-L1 value was an independent risk factor for tumor recurrence.

Conclusions

We presented an image analysis model to quantify the spatial intratumor heterogeneity of protein expression in tumor tissues. This model demonstrated that the spatial intratumor heterogeneity of PD-L1 expression in surgically resected NSCLC predicts poor patient outcomes.
The unique risk factor profile of triple-negative breast cancer: a comprehensive meta-analysis
Authors: Kumar Nitya , Ehsan Sarah , Banerjee Shahana , Fernandez Perez Claudia , Lhuilier Isabelle , Neuner Jillian , Friebel-Klingner Tara , Fayanju Oluwadamilola M , Nair Bindhu , Niinuma Sara Anjum , Nampoothiri Shivangi , McCarthy Anne Marie
Published: March 2024

Background

Triple-negative breast cancer (TNBC) has a poor prognosis compared with other breast cancer subtypes. This systematic review and meta-analysis examines whether known risk factors for breast cancer are also associated with TNBC in adult women.

Methods

EMBASE, Medline, SCOPUS, and gray literature were queried with no limit on the date or language of publication. The exposures of interest included parity, breastfeeding, duration of breastfeeding, age at menarche, age at first live birth, oral contraceptive (OC) use, duration of OC use, use of menopausal hormone therapy (MHT), family history, body mass index (BMI), alcohol use, smoking, and breast density. The main outcome of interest was TNBC. Study quality was determined using the Newcastle-Ottawa scale for case control studies and cohort studies. We estimated weighted odds ratios from random effects models to study the exposure–outcome associations. Protocol was registered under the number: PROSPERO 2021 CRD42021254594.

Results

Thirty-three studies were included. Family history, longer duration of OC use, and higher breast density were significantly associated with increased risk for TNBC, whereas later age at menarche, later age at first birth, and breastfeeding were protective against TNBC. Parity, MHT, alcohol, smoking, and BMI were not significantly associated with TNBC overall, but higher parity was associated with higher risk among Black women.

Conclusion

Our findings highlight that TNBC has a distinct risk factor profile compared with overall breast cancer. This can be the foundational work in identification of actionable TNBC risk factors to improve prevention and early detection of these poor prognosis breast tumors.
Psychosocial characteristics of workers with irritable bowel syndrome and its relationship with abdominal symptoms and work productivity
Authors: Sugaya Nagisa , Izawa Shuhei , Sasaki Takeshi
Published: March 2024

Objectives:

This study aimed to validate the psychosocial characteristics and work-related challenges faced by workers exhibiting symptoms of irritable bowel syndrome (IBS) and to clarify the factors that exacerbate abdominal symptoms or hinder work productivity by focusing on IBS-related cognitive-behavioral factors and job-related stressors.

Methods:

An online survey was conducted from October 5 to October 20, 2023, among workers in Japan aged 20-49 years with more than 30 hours of work per week, excluding managers and the self-employed. The data were obtained from 1062 participants (including 551 women and 329 individuals with IBS).

Results:

The levels of depression in the IBS group were significantly higher than those in the Non-IBS group (P < .05). Cognitive-behavioral variables associated with abdominal symptoms were positively correlated with work productivity (r = 0.367-0.483, P < .001). Hierarchical multiple regression analysis with IBS symptoms and work productivity as dependent variables revealed a significant effect of the interaction between maladaptive cognition related to abdominal symptoms and job control on IBS symptoms (β = −.164, P = .002). The association between maladaptive cognition and IBS symptoms was more pronounced when job control was lower. Additionally, IBS symptoms (β = .130-.214, P < .05), maladaptive cognition (β = .196-.233, P < .01), and job overload (β = .106-.108, P < .05) significantly influenced work productivity.

Conclusions:

Maladaptive cognition regarding abdominal symptoms may lead to more severe abdominal symptoms in situations with a low sense of work control. Beyond cognitive-behavioral interventions for IBS, enhancing the sense of control over work environments is expected to contribute to alleviating abdominal symptoms and, consequently, enhancing work productivity.
Group A Streptococcus pharyngitis in Children: New Perspectives on Rapid Diagnostic Testing and Antimicrobial Stewardship
Authors: Cohen Jérémie F , Tanz Robert R , Shulman Stanford T
Published: March 2024

The most common cause of bacterial pharyngitis is Group A Streptococcus (GAS). Accurate diagnosis of GAS pharyngitis is crucial to identify children who would benefit from antibiotic treatment. Rapid diagnosis has the potential to reduce antibiotic overuse. Current national guidelines differ in their recommendations for GAS testing. While rapid antigen detection tests (RADTs) are widely used, their sensitivity is considered too low for stand-alone testing by several expert bodies. Newer molecular tests using nucleic acid amplification show higher accuracy and fast results, but their cost, complexity, and very high sensitivity may limit widespread adoption. This review provides up-to-date evidence regarding rapid diagnostic testing and antimicrobial stewardship in children with sore throat. We discuss discrepancies across GAS testing guidelines at the international level, patient selection for testing for GAS, rapid test accuracy, and the potential role of rapid GAS tests to promote antibiotic stewardship, with emphasis on emerging rapid molecular tests.
Evolutionary insights into strategy shifts for the safe and effective accumulation of ascorbate in plants
Authors: Maruta Takanori , Tanaka Yasuhiro , Yamamoto Kojiro , Ishida Tetsuya , Hamada Akane , Ishikawa Takahiro
Published: March 2024

Plants accumulate high concentrations of ascorbate, commonly in their leaves, as a redox buffer. While ascorbate levels have increased during plant evolution, the mechanisms behind this phenomenon are unclear. Moreover, has the increase in ascorbate concentration been achieved without imposing any detrimental effects on the plants? In this review, we focus on potential transitions in two regulatory mechanisms related to ascorbate biosynthesis and the availability of cellular dehydroascorbate (DHA) during plant evolution. The first transition might be that the trigger for the transcriptional induction of VTC2, which encodes the rate-limiting enzyme in ascorbate biosynthesis, has shifted from oxidative stress (in green algae) to light/photosynthesis (in land plants), probably enabling the continuous accumulation of ascorbate under illumination. This could serve as a preventive system against the unpredictable occurrence of oxidative stress. The second transition might be that DHA-degrading enzymes, which protect cells from the highly reactive DHA in green algae and mosses, have been lost in ferns or flowering plants. Instead, flowering plants may have increased glutathione concentrations to reinforce the DHA reduction capacity, possibly allowing ascorbate accumulation and avoiding the toxicity of DHA. These potential transitions may have contributed to strategies for plants’ safe and effective accumulation of ascorbate.
Digital assemblages with AI for creative interpretation of short stories
Authors: O'Halloran Kieran
Published: March 2024

I demonstrate an approach fostering inventive interpretation of short stories in Literary Studies and higher education generally. It involves constructing an ‘assemblage’—at its simplest, an evolving network of unusual connections for creative outcome. The assemblage of this article combines freshly located research literature, directly and indirectly related to a story’s themes, and/or the personality type of protagonists. Importantly, this assemblage also utilizes text analysis software revealing the relatively invisible (e.g. (in)frequent words, parts of speech, and topics) and Large Language Model (LLM) Generative AI to enrich the interpretation. The use of all these elements helps productively exceed initial intuitions about the story, facilitating creativity. I model the approach using Edgar Allan Poe’s short story, The Black Cat, whose protagonist is a homicidal psychopath. Specifically, the assemblage here includes relevant software-based research (a corpus analysis of homicidal psychopathic language), non-software-based research (psychoanalytical literary criticism of The Black Cat using the empirically validated concept of transference), text analysis software (WMatrix and Datayze), and the LLM Generative AI, ‘ChatGPT’ (using the freely available LLM GPT-3.5). One use of this approach is as a pedagogy in Literary Studies employing text analysis software (e.g. on a digital stylistics course). Yet given creative adaptability is a key 21st-century skill, with digital literacy—including the use of Generative AI—an important contemporary competence, and with the short story genre universally known, I highlight too the utility of this approach as a university-wide pedagogy for enhancing creative thinking.
Kinetically coupled scalar fields model and cosmological tensions
Authors: Liu Gang , Zhou Zhihuan , Mu Yuhao , Xu Lixin
Published: March 2024

In this paper, we investigate the kinetically coupled early dark energy (EDE) and scalar field dark matter to address cosmological tensions. The EDE model presents an intriguing theoretical approach to resolving the Hubble tension, but it exacerbates the large-scale structure tension. We consider the interaction between dark matter and EDE, such that the drag of dark energy on dark matter suppresses structure growth, which can alleviate large-scale structure tension. We replace cold dark matter with scalar field dark matter, which has the property of suppressing structure growth on small scales. We employed the Markov Chain Monte Carlo method to constrain the model parameters, our new model reveals a non-zero coupling constant of 0.030 ± 0.026 at a 68 per cent confidence level. The coupled model yields the Hubble constant value of $72.38^{+0.71}_{-0.82}$ km s−1 Mpc−1, which resolves the Hubble tension. However, similar to the EDE model, it also obtains a larger S8 value compared to the ΛCDM model, further exacerbating the large-scale structure tension. The EDE model and the new model yield the best-fitting values of 0.8316 and 0.8146 for S8, respectively, indicating that the new model partially alleviates the negative effect of the EDE model. However, this signature disappears when comparing marginalized posterior probabilities, and both models produce similar results. The values obtained from the EDE model and the new model are $0.822^{+0.011}_{-0.0093}$ and $0.819^{+0.013}_{-0.0092}$, respectively, at a 68 per cent confidence level.
In-clinic Versus Online Recruitment of Women With a History of Cervical Intraepithelial Neoplasia or Cervical Cancer to a Smoking Cessation Trial: A Post hoc Comparison of Participant Characteristics, Study Retention, and Cessation Outcomes
Authors: Fennell Bethany Shorey , Jones Sarah R , Sutton Steven K , Hoogland Charles E , Cottrell-Daniels Cherell , Wetter David W , Shih Ya-Chen Tina , Simmons Vani N , Stephens Yesenia P , Vidrine Damon J , Vidrine Jennifer I
Published: March 2024

Introduction

Recruiting special populations to smoking cessation trials is challenging and approaches beyond in-clinic recruitment may be beneficial. This secondary analysis of data from a smoking cessation RCT for individuals with a history of cervical cancer or cervical intraepithelial neoplasia (CIN) explored differences associated with in-clinic vs. online recruitment.

Aims and Methods

Participants were recruited from clinics within a university-based NCI-designated cancer center (n = 87) and online nationally via Facebook (n = 115). Baseline measures included sociodemographics, smoking history, and cancer or CIN history. Study retention and smoking abstinence were assessed 12 months post-baseline. Group differences in baseline characteristics were evaluated. Retention and abstinence were evaluated while controlling for group differences and predictors.

Results

Participants recruited online (vs. in-clinic) had higher educational attainment (p = .01) and health literacy (p = .003). They were more likely to have CIN versus cancer, to be further from the time of diagnosis, and to have completed active treatment (p values < .001). While controlling for these group differences and independent predictors, retention was higher among participants recruited online (log-likelihood χ2(1) = 11.41, p < .001). There were no recruitment differences in self-reported (p = .90) or biochemically confirmed smoking abstinence (p = .18).

Conclusions

Compared to individuals recruited in-person, individuals recruited online were more educated, had higher health literacy, and presented with a different clinical profile (ie, more likely to have CIN vs. cancer and to have completed active treatment). There were few differences in participant characteristics between recruitment approaches, and no differences on any smoking-related variables. Online recruitment has the potential to improve enrollment of cancer survivors in smoking cessation trials.

Implications

People with a history of CIN or cervical cancer recruited to a smoking cessation RCT online (vs. in-clinic) were more likely to have a diagnosis of CIN versus cancer and were more educated and health literate. Participants recruited online were more likely to be retained in the study and there were no differences in smoking abstinence rates at 12 months. Incorporating online recruitment increased the reach of tobacco treatment efforts to a larger and more diverse sample. This could reduce the burden of tobacco-related disease, improve CIN and cancer treatment outcomes, and reduce secondary malignancies and morbidity among this underserved group.
Assessing the Level of Poverty and Utilization of Government Social Programs Among Tobacco Farmers in Indonesia
Authors: Sahadewo Gumilang Aryo , Lencucha Raphael , Bandara Shashika , Drope Jeffrey , Witoelar Firman
Published: March 2024

Introduction

Studies examining profit suggest that former tobacco farmers do as well or better than current tobacco farmers. Research has yet to examine the relationship among current and former tobacco farmers, poverty, and receipt of government social assistance. This type of research is critical to understanding the direct and indirect subsidization of tobacco growing. This study analyzed tobacco farmers’ poverty levels and receipt of government social assistance programs.

Aims and Methods

We designed and conducted an original four-wave economic survey of current and former tobacco farming households in Indonesia between 2016 and 2022. We then used descriptive analysis and probit regression for panel data to estimate the relationship between tobacco farming and poverty status.

Results

Tobacco farmers’ per capita income and poverty rates vary across years. The poverty rate was significantly higher in the year with a higher-than-normal rainfall as it negatively affected farming outcomes. During this year, the poverty rate among current tobacco farmers was also higher than that of former tobacco farmers. Regression estimates from the panel data confirm the association between tobacco farming and the likelihood of being poor. We also found a high share of current tobacco farmers who receive government social assistance programs, such as cash transfer programs and a universal healthcare program.

Conclusions

Our findings show high poverty rates—particularly during bad farming years—and high rates of government social assistance among tobacco farmers. The high rates of government assistance among tobacco farmers living in poverty show that the government is indirectly subsidizing the tobacco industry.
Two-Year Longitudinal Study Reveals That Long COVID Symptoms Peak and Quality of Life Nadirs at 6–12 Months Postinfection
Authors: Demko Zoe O , Yu Tong , Mullapudi Sarika K , Varela Heslin M Gabriela , Dorsey Chamia A , Payton Christine B , Tornheim Jeffrey A , Blair Paul W , Mehta Shruti H , Thomas David L , Manabe Yukari C , Antar Annukka A R
Published: March 2024

Background

Few longitudinal studies available characterize long COVID outcomes out to 24 months, especially in people with nonsevere acute coronavirus disease 2019 (COVID-19). This study sought to prospectively characterize incidence and duration of long COVID symptoms and their association with quality of life (QoL) from 1–24 months after mild-to-moderate COVID-19 using validated tools in a diverse cohort of unvaccinated people infected with SARS-CoV-2 in 2020.

Methods

At 1–3, 6, 12, 18, and 24 months post-COVID-19, 70 participants had orthostatic vital signs measured, provided blood, and completed surveys characterizing symptoms, QoL, and return to pre-COVID-19 health and activities using validated tools (FLU-PRO+, Fatigue Severity Scale, Insomnia Severity Index, General Practitioner Assessment of Cognition, Patient Health Questionnaire Depression 8-Item, Generalized Anxiety Disorder 7-Item, 36-Item Short-Form Health Survey, EuroQol EQ-5D-5L).

Results

During the study period, 33% of participants experienced long COVID (had not returned to pre-COVID-19 health status and reported at least 1 symptom >90 days postinfection); 8% had not returned to their pre-COVID-19 health status 24 months postinfection. Long COVID symptoms peaked 6 months post-COVID-19, frequently causing activity limitations. Having long COVID was significantly associated with decreased QoL in multiple domains. Frequencies of orthostatic hypotension and tachycardia reflected levels reported in the general population. Within-person weight increased significantly between months 1 and 6. Long COVID was associated with pre-COVID-19 obesity and hyperlipidemia, but not with high-sensitivity C-reactive protein levels 1–3 months postinfection.

Conclusions

Long COVID occurs in a significant proportion of unvaccinated people, even if the acute illness was not severe. Long COVID prevalence peaked 6–12 months post-COVID-19, and a small proportion of participants still reported not returning to their pre-COVID-19 health status 24 months post-COVID-19.
All-cause and Infection-attributable Mortality Amongst Adults With Bloodstream Infection—a Population-based Study
Authors: Underwood Jonathan , Griffiths Rowena , Gillespie David , Akbari Ashley , Ahmed Haroon
Published: March 2024

Background

Bloodstream infections (BSIs) are common, life-threatening infections. However, it remains unclear whether deaths following BSIs are primarily from uncontrolled infection or underlying comorbidities. We aimed to determine the overall mortality, infection-attributable mortality, and causes of death for four leading BSI pathogens.

Methods

This retrospective cohort study was conducted within the Secure Anonymized Information Linkage Databank, containing anonymized population-scale electronic health record data for Wales, UK. We included adults with Escherichia coli, Klebsiella spp, Pseudomonas aeruginosa, and Staphylococcus aureus BSI between 2010 and 2022 using linked data from Public Health Wales and the Office for National Statistics. Thirty-day all-cause and sepsis-specific mortality, as a proxy for infection-attributable mortality, were compared using Cox proportional hazards and competing risk regression, respectively.

Results

We identified 35 691 adults with BSI (59.6% E coli). Adjusted analyses revealed that all organisms had a higher 30-day mortality versus E coli with Pseudomonas aeruginosa the highest (hazard ratio, 1.96 [1.76–2.17], P < .001). Cancer was the leading cause of death following BSIs for all organisms, particularly deaths occurring between 30 and 90 days (35.9%). A total of 25.5% of deaths within 30 days involved sepsis. Methicillin-resistant Staphylococcus aureus was associated with the highest sepsis mortality versus E coli (hazard ratio, 2.56 [2.10–3.12], P < .001). Peak C-reactive protein was positively associated with increased sepsis mortality (P < .001).

Conclusions

This population-level study challenges the assumption that most deaths following BSIs are directly attributable to uncontrolled infection, particularly subacutely more than 30 days from BSI. Our findings underscore the need for reevaluating clinical trial design and developing better preventive strategies for BSIs.
Peak energy–isotropic luminosity correlation and jet opening angle evolution in Swift-BAT short GRBs with soft-tail emission
Authors: Ogino Naoki , Yonetoku Daisuke , Arimoto Makoto , Sawano Tatsuya , Hamidani Hamid
Published: March 2024

Some short gamma-ray bursts (SGRBs) exhibit a short-duration and spectral hard emission (referred to as a “hard spike”) followed by a slightly longer soft emission (known as a “soft tail”). We identified nine SGRBs with the known redshift in the Swift/BAT gamma-ray burst catalog by specifically searching for the soft tail. We found that the spectra of these SGRBs can be described as a cutoff power-law model for the hard spike and the soft tail, and both show a time variation keeping the Epeak–Liso correlation. This suggests that the emission mechanisms of both phenomena are identical. Furthermore, we found a trend of luminosity evolution as a function of redshift. This phenomenon suggests that these bursts originate from sources that are intrinsically bright and/or energy-density-concentrated within a narrower jet at higher redshift. We demonstrate that the average jet opening angle, derived from the jet break, can be explained by considering a model based on a strongly redshift-dependent jet opening angle.
Glucose-G protein signaling plays a crucial role in tomato resilience to high temperature and elevated CO2
Authors: Wang Jiao , Luo Qian , Liang Xiao , Liu Hua , Wu Changqi , Fang Hanmo , Zhang Xuanbo , Ding Shuting , Yu Jingquan , Shi Kai
Published: March 2024

Global climate change is accompanied by carbon dioxide (CO2) enrichment and high temperature (HT) stress; however, how plants adapt to the combined environments and the underlying mechanisms remain largely unclear. In this study, we show that elevated CO2 alleviated plant sensitivity to HT stress, with significantly increased apoplastic glucose (Glc) levels in tomato (Solanum lycopersicum) leaves. Exogenous Glc treatment enhanced tomato resilience to HT stress under ambient CO2 conditions. Cell-based biolayer interferometry, subcellular localization, and Split-luciferase assays revealed that Glc bound to the tomato regulator of G protein signaling 1 (RGS1) and induced RGS1 endocytosis and thereby RGS1-G protein α subunit (GPA1) dissociation in a concentration-dependent manner. Using rgs1 and gpa1 mutants, we found that RGS1 negatively regulated thermotolerance and was required for elevated CO2-Glc-induced thermotolerance. GPA1 positively regulated the elevated CO2-Glc-induced thermotolerance. A combined transcriptome and chlorophyll fluorescence parameter analysis further revealed that GPA1 integrated photosynthesis- and photoprotection-related mechanisms to regulate thermotolerance. These results demonstrate that Glc–RGS1–GPA1 signaling plays a crucial role in the elevated CO2-induced thermotolerance in tomato. This information enhances our understanding of the Glc-G protein signaling function in stress resilience in response to global climate change and will be helpful for genetic engineering approaches to improve plant resilience.
Measuring Policy Learning: Challenges and Good Practices
Authors: Zaki Bishoy Louis , Radaelli Claudio M
Published: March 2024

How can we empirically identify and measure policy learning? Through a problematized review, we develop a novel perspective to identifying, explaining, and addressing interconnected ontological, theoretical-contextual, and consequently, methodological challenges in the measurement of policy learning. We de-compose measurement as a three-fold endeavor, concerning: conceptual foundations, the design of measurement instruments, and their deployment in empirical research. Then, we synthesize exemplars of good practice across these three stages and offer a set of recommendations for the refinement of policy learning measurement, while maintaining theoretical and methodological pluralism.
Anti-Asian Racism and the Rise of Hawkish Mass Opinion in China
Authors: Kim D G
Published: March 2024

The dramatic increase in anti-Asian racial violence in the United States during the pandemic has sparked debates on issues of domestic racial justice and deep-seated racism against Asian Americans. Political scientists, however, have paid relatively little attention to how growing anti-Asian racism might affect contemporary U.S.-China relations, especially by shaping foreign policy discourses and public opinion in China. In this article, I investigate the way the Chinese state media discuss the issue and how such top-down discourses shape mass political attitudes and foreign policy preferences in China. Analyzing the Chinese state media coverage of anti-Asian racial violence, I first find that Chinese official narratives frame the issue as a manifestation of both racially motivated American foreign policy and problems with American democracy. By conducting a nationwide survey experiment in China, I then examine the impact of such top-down political rhetoric on public support for hawkish foreign policies. I find that these political narratives significantly boost racialized and nationalist sentiments among the Chinese masses, which, in turn, garner greater public support for hawkish foreign policy. This article contributes to understanding the foreign policy implications of anti-Asian racism after the global pandemic and the potential racialization of Sino-American great-power competition.
One-step biofabrication of liquid core—GelMa shell microbeads for in situ hollow cell ball self-assembly
Authors: Chen Jianwei , Liu Zeyang , Wang Zixian , Zhang Xiuxiu , Zhang Yi , Zhan Zhen , Gong Xiaohua , Xu Tao
Published: March 2024

There are many instances of hollow-structure morphogenesis in the development of tissues. Thus, the fabrication of hollow structures in a simple, high-throughput and homogeneous manner with proper natural biomaterial combination is valuable for developmental studies and tissue engineering, while it is a significant challenge in biofabrication field. We present a novel method for the fabrication of a hollow cell module using a coaxial co-flow capillary microfluidic device. Sacrificial gelatin laden with cells in the inner layer and GelMa in the outer layer are used via a coaxial co-flow capillary microfluidic device to produce homogenous micro-beads. The overall and core sizes of core–shell microbeads were well controlled. When using human vein vascular endothelial cells to demonstrate how cells line the inner surface of core–shell beads, as the core liquifies, a hollow cell ball with asymmetric features is fabricated. After release from the GelMa shell, individual cell balls are obtained and deformed cell balls can self-recover. This platform paves way for complex hollow tissue modeling in vitro, and further modulation of matrix stiffness, curvature and biochemical composition to mimic in vivo microenvironments.
Plaintiff S99: Rewriting Refugee Law Through a Trauma-Informed Lens
Authors: Hambly Jessica , Sanmuhanathan Neeraja
Published: March 2024

Plaintiff S99/2016 v. Minister for Immigration and Border Protection [2016] FCA 483 was a decision of the Australian Federal Court concerning a woman (referred to in the decision as “Plaintiff S99”) who had been recognised as a refugee in the Republic of Nauru, having been transferred there by Australia under an offshore processing agreement. The decision in Plaintiff S99 established an important precedent that many refugees and asylum-seekers in Australia’s offshore processing centres on Manus and Nauru, including children with severe health problems, subsequently relied upon to be transferred to Australia for critical medical care. Drawing on the recent turn towards trauma-informed practice across other areas of law and policy, we ask: what does this mean for refugees, and, more specifically, what does this mean for judgment writing in refugee law? We use our rewrite of Plaintiff S99 to highlight aspects of the decision and its legacy that continue to silence and erase the experiences of refugees, especially refugee women, and frequently contribute to compounding their trauma. Our contribution calls for a reorientation towards “do no harm” principles, which lie at the heart of trauma and violence-informed practice. In particular, our approach to rewriting S99 aims at foregrounding dignity and safety, promoting respect for physical and mental health, and centring the voice, experience, and longer-term protection needs of refugees.
Prospective evaluation of sexual health following radical cystectomy due to bladder cancer
Authors: Nolting Julia , Nitzsche Romy , Kiss Bernhard , Hakenberg Oliver W , Schneidewind Laila
Published: March 2024

Background

There is a lack of data concerning sexual health following open radical cystectomy (RC), especially in elderly patients and women.

Aim

To describe sexual health and its impact on general health as well as survival in patients undergoing standard open RC for the treatment of bladder cancer (BC). Due to limited data, subgroup analysis for elderly patients and women was performed.

Methods

A prospective noninterventional clinical study was performed evaluating sexual health in RC with any kind of urinary diversion due to BC with a follow-up of 12 months after RC. The study was approved by the local ethics review board (A 2021-0175) and was registered at the German Clinical Trial Register (DRKS00026255). Assessment of sexual health was done with the following validated questionnaires: EORTC QLQ-C30 (for quality of life; European Organisation for Research and Treatment of Cancer), EORTC SH22 (for sexual health), and IIEF-5 (5-item International Index of Erectile Function).

Outcomes

The standard measurements of EORTC QLQ-C30, EORTC SH22, and IIEF-5 as well as overall survival.

Results

Thirty-two patients participated in the study with a mean age of 71.5 years (SD, 9.7): 25 (78.1%) were male and 7 (21.9%) were female. Overall there is a heterogenic picture for sexual health in the study population, but sexual satisfaction is significantly higher prior to surgery while the importance of a sex life stays high and stable. Interestingly, the general health score is significantly correlated to sexual satisfaction (Pearson’s correlation; r = 0.522, P = .002) preoperatively but not following surgery: r = 0.103 (P = .665) after 3 months, r = 0.478 (P = .052) after 6 months, r = 0.276 (P = .302) after 9 months, and r = 0.337 (P = .202) after 12 months. The importance of a sex life is still essential for the patients, especially when recovering from RC; nearly the same can be reported for elderly patients. Unfortunately, the data for women are too limited to report robust results.

Clinical Implications

Evaluation, advice, and monitoring of sexual health must be integrated into clinical practice, particularly in women.

Strengths and Limitations

At least to our knowledge, this is the first systematic prospective evaluation of sexual health in patients with BC receiving RC. Due to the small sample size, there is a risk of selection bias.

Conclusion

Sexual health is important for patients with BC receiving RC, and it is an essential part of quality of life, especially in elderly patients.
Sleep-slow oscillation-spindle coupling precedes spindle-ripple coupling during development
Authors: Fechner Julia , Contreras María P , Zorzo Candela , Shan Xia , Born Jan , Inostroza Marion
Published: March 2024

Study Objectives

Sleep supports systems memory consolidation through the precise temporal coordination of specific oscillatory events during slow-wave sleep, i.e. the neocortical slow oscillations (SOs), thalamic spindles, and hippocampal ripples. Beneficial effects of sleep on memory are also observed in infants, although the contributing regions, especially hippocampus and frontal cortex, are immature. Here, we examined in rats the development of these oscillatory events and their coupling during early life.

Methods

EEG and hippocampal local field potentials were recorded during sleep in male rats at postnatal days (PD)26 and 32, roughly corresponding to early (1–2 years) and late (9–10 years) human childhood, and in a group of adult rats (14–18 weeks, corresponding to ~22–29 years in humans).

Results

SO and spindle amplitudes generally increased from PD26 to PD32. In parallel, frontocortical EEG spindles increased in density and frequency, while changes in hippocampal ripples remained nonsignificant. The proportion of SOs co-occurring with spindles also increased from PD26 to PD32. Whereas parietal cortical spindles were phase-locked to the depolarizing SO-upstate already at PD26, over frontal cortex SO-spindle phase-locking emerged not until PD32. Co-occurrence of hippocampal ripples with spindles was higher during childhood than in adult rats, but significant phase-locking of ripples to the excitable spindle troughs was observed only in adult rats.

Conclusions

Results indicate a protracted development of synchronized thalamocortical processing specifically in frontocortical networks (i.e. frontal SO-spindle coupling). However, synchronization within thalamocortical networks generally precedes synchronization of thalamocortical with hippocampal processing as reflected by the delayed occurrence of spindle-ripple phase-coupling.
Moringa oleifera leaves ethanolic extract counteracts cortical neurodegeneration induced by aluminum chloride in rats
Authors: Hindawy Rabab Fawzy , Manawy Samia M , Nafea Ola Elsayed , Abdelhameed Abeer A , Hendawi Fatma Fawzi
Published: March 2024

Background

Aluminum, a well-recognized neurotoxin, is implicated in various neurodegenerative disorders. Moringa oleifera (M. oleifera), known as a miracle tree, is utilized as a functional food and nutritional supplement. This study investigates the potential preventive effects of M. oleifera extract on aluminum chloride (AlCl3)-induced cortical neurodegeneration in rats.

Materials and methods

Therefore, 24 adult male Wistar rats were randomly divided into four distinct groups: negative control, M. oleifera extract (MOE), AlCl3, and AlCl3 + MOE. Treatments were administered orally for 28 consecutive days. Cognitive performance, brain oxidative/nitrosative stress, neuroinflammation, apoptotic-cell death, and associated histopathological alterations were assessed.

Results

Our results showed that MOE improved spatial learning and memory, enhanced antioxidant superoxide dismutase enzyme activity, antagonized nitrosative stress, reduced inflammatory cytokines (tumor necrosis factor-alpha and interleukin-6), decreased caspase-3, increased Bcl-2, and facilitated repair of cortical and hippocampal structures.

Conclusions

We concluded that MOE exhibits protective effects against cortical neurodegeneration, making it a promising supplement to counteract aluminum-induced neurotoxic effects.
Tannic acid ameliorates the hazards effect of beryllium induced neuro-alterations and oxidative stress in adult male rats
Authors: Rezk Mohamed M
Published: March 2024

Background

Tannic acid (TA) is one of the most consumed and famous polyphenols with a widespread attention in the medical field according to its unique structural, pharmaceutical, physicochemical, antioxidant and other biological features. A rare study was conducted on the hazard effect of beryllium (Be) on the central nervous system.

Aims

This study aims to show the ability of beryllium to cross the blood brain barrier. Demonstrate the effect of beryllium and tannic acid separately or with each other on brain ions (Na+, K+, Ca++) and on norepinephrine, dopamine, serotonin, finally on the glutathione and malondialdehyde.

Animals grouping

Seventy-two rats were divided into four groups as control, Be, TA, and Be+TA where Be was injected intraperitoneally as 1 mg/Kg b. wt, TA was orally administrated as 5% in aquas solution.

Results

The administration of beryllium showed its ability to cross the blood brain barrier and accumulated in cortex > cerebellum>hypothalamus also, a significant increase in Na+, Ca++ cooperated with a significant decrease in K+ ions content was observed. Norepinephrine, dopamine, and serotonin showed a general significant decrease in their content joined with a significant decrease in glutathione (GSH) and elevation in malondialdehydes (MDA) because of Be intoxication. On the other hands the daily oral administration of tannic acid showed a general significant decrease in Na+, Ca++ ions content parallel with a significant increase K+ also, a non-significant change in the three measured neurotransmitters was noticed.

Conclusion

Tannic acid showed a mitigation effect against Be intoxication which may regarded to the tannic acid antioxidant, chelating effect.
Effects of the Reynolds number on train aerodynamics considering air compressibility: a wind tunnel study
Authors: Huang Zhixiang , Li Wenhui , Chen Li
Published: March 2024

The study of Reynolds number (Re) effects is crucial for optimizing train aerodynamics, enhancing safety and reducing energy consumption of high-speed trains. In this paper, a wind tunnel test was carried out to investigate the influence of Re on the train aerodynamic performance considering the shift of the air compressibility, which has not yet been explored to date. The test was conducted in a low-speed and large-scale tunnel with a stationary floor and the vehicle model was based on a 1/8th scaled train with three units. The Re ranges from 0.75 × 106∼3.12 × 106 by accelerating the uniform wind speed from U = 27.8 m/s to U = 115 m/s with zero-yaw. The Mach number (Ma) of the maximum speed scenario has exceeded 0.3, indicating that the airflow can be considered as being in the compressible range. The results show that the aerodynamic characteristics of high-speed trains exhibit a self-similarity region of Re, which is dependent on the flow velocity. The aerodynamic loads change little when Re ≥ 1.51 × 106, which corresponds to U ≥ 55.6 m/s. Therefore, the compressibility of the airflow within the range up to U = 115 m/s has a negligible effect on aerodynamic loads, and can thus be disregarded. However, the surface pressure significantly decreases when the incoming flow surpasses 0.3 Ma and transitions into a compressible state. While the compressibility has a relatively minor impact on macroscopic aerodynamics, it cannot be overlooked when considering detailed flow field, such as surface pressure.
Too Hard, Too Easy, or Just Right: The Productivity of Schooling and the Match between Child Skill and School Complexity
Authors: Castro Juan F , Villacorta Lucciano
Published: March 2024

This study proposes a novel way of modeling the heterogeneous effects of schooling based on the notion that learning is maximized when the skill of the child matches the complexity of the learning experiences at school. It offers direct evidence about the importance of this match using longitudinal information on test scores and schooling attained by children from Peru, India, and Vietnam. Using data from Peru, it also finds that the relation between the effect of schooling and early childhood skill can follow an inverted-U shape. Increasing early childhood skill will raise the productivity of the school up to the point where it matches school complexity. Further increases in child skill, however, will reduce the productivity of schooling as they will widen the mismatch. If one relates the quality of schools to the amount of learning they produce, this framework predicts that quality gains can be achieved by reducing these mismatches.