bluuebunny/crossref_metadata_2025 huggingface dataset:
Proteomic Analysis of HCC-1954 and MCF-7 Cell Lines Highlights Crosstalk between αv and β1 Integrins, E-Cadherin and HER-2
Authors: de Abreu Pereira Denise , Sandim Vanessa , Fernandes Thais F. B. , Almeida Vitor Hugo , Rocha Murilo Ramos , do Amaral Ronaldo J. F. C. , Rossi Maria Isabel D. , Kalume Dário Eluan , Zingali Russolina B.
Published: September 2022

Overexpression of human epidermal growth factor receptor-2 (HER-2) occurs in 20% of all breast cancer subtypes, especially those that present the worst prognostic outcome through a very invasive and aggressive tumour. HCC-1954 (HER-2+) is a highly invasive, metastatic cell line, whereas MCF-7 is mildly aggressive and non-invasive. We investigated membrane proteins from both cell lines that could have a pivotal biological significance in metastasis. Membrane protein enrichment for HCC-1954 and MCF-7 proteomic analysis was performed. The samples were analysed and quantified by mass spectrometry. High abundance membrane proteins were confirmed by Western blot, immunofluorescence, and flow cytometry. Protein interaction prediction and correlations with the Cancer Genome Atlas (TCGA) patient data were conducted by bioinformatic analysis. In addition, β1 integrin expression was analysed by Western blot in cells upon trastuzumab treatment. The comparison between HCC-1954 and MCF-7 membrane-enriched proteins revealed that proteins involved in cytoskeleton organisation, such as HER-2, αv and β1 integrins, E-cadherin, and CD166 were more abundant in HCC-1954. β1 integrin membrane expression was higher in the HCC-1954 cell line resistant after trastuzumab treatment. TCGA data analysis showed a trend toward a positive correlation between HER-2 and β1 integrin in HER-2+ breast cancer patients. Differences in protein profile and abundance reflected distinctive capabilities for aggressiveness and invasiveness between HCC-1954 and MCF-7 cell line phenotypes. The higher membrane β1 integrin expression after trastuzumab treatment in the HCC-1954 cell line emphasised the need for investigating the contribution of β1 integrin modulation and its effect on the mechanism of trastuzumab resistance.
Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease
Authors: Genkel Vadim , Dolgushin Ilya , Baturina Irina , Savochkina Albina , Nikushkina Karina , Minasova Anna , Pykhova Lubov , Sumerkina Veronika , Kuznetsova Alla , Shaposhnik Igor
Published: September 2022

Background: Current data on the possible involvement of aging neutrophils in atherogenesis are limited. This study aimed to research the diagnostic value of aging neutrophils in their relation to subclinical atherosclerosis in statin-naïve patients without established atherosclerotic cardiovascular diseases (ASCVD). Methods: The study was carried out on 151 statin-naïve patients aged 40–64 years old without ASCVD. All patients underwent duplex scanning of the carotid arteries, lower limb arteries and abdominal aorta. Phenotyping and differentiation of neutrophil subpopulations were performed through flow cytometry (Navios 6/2, Beckman Coulter, USA). Results: The number of CD62LloCXCR4hi-neutrophils is known to be significantly higher in patients with subclinical atherosclerosis compared with patients without atherosclerosis (p = 0.006). An increase in the number of CD62LloCXCR4hi-neutrophils above cut-off values makes it possible to predict atherosclerosis in at least one vascular bed with sensitivity of 35.4–50.5% and specificity of 80.0–92.1%, in two vascular beds with sensitivity of 44.7–84.4% and specificity of 80.8–33.3%. Conclusion: In statin-naïve patients 40–64 years old without established ASCVD with subclinical atherosclerosis, there is an increase in circulating CD62LloCXCR4hi-neutrophils. It was also concluded that the increase in the number of circulating CD62LloCXCR4hi-neutrophils demonstrated moderate diagnostic efficiency (AUC 0.617–0.656) in relation to the detection of subclinical atherosclerosis, including polyvascular atherosclerosis.
The Ambivalence of Connexin43 Gap Peptides in Cardioprotection of the Isolated Heart against Ischemic Injury
Authors: Falck Aleksander Tank , Lund Bjarte Aarmo , Johansen David , Lund Trine , Ytrehus Kirsti
Published: September 2022

The present study investigates infarct-reducing effects of blocking ischemia-induced opening of connexin43 hemichannels using peptides Gap19, Gap26 or Gap27. Cardioprotection by ischemic preconditioning (IPC) and Gap peptides was compared, and combined treatment was tested in isolated, perfused male rat hearts using function and infarct size after global ischemia, high-resolution respirometry of isolated mitochondrial and peptide binding kinetics as endpoints. The Gap peptides reduced infarct size significantly when given prior to ischemia plus at reperfusion (Gap19 76.2 ± 2.7, Gap26 72.9 ± 5.8 and Gap27 71.9 ± 5.8% of untreated control infarcts, mean ± SEM). Cardioprotection was lost when Gap26, but not Gap27 or Gap19, was combined with triggering IPC (IPC 73.4 ± 5.5, Gap19-IPC 60.9 ± 5.1, Gap26-IPC 109.6 ± 7.8, Gap27-IPC 56.3 ± 8.0% of untreated control infarct). Binding stability of peptide Gap26 to its specific extracellular loop sequence (EL2) of connexin43 was stronger than Gap27 to its corresponding loop EL1 (dissociation rate constant Kd 0.061 ± 0.004 vs. 0.0043 ± 0.0001 s−1, mean ± SD). Mitochondria from IPC hearts showed slightly but significantly reduced respiratory control ratio (RCR). In vitro addition of Gap peptides did not significantly alter respiration. If transient hemichannel activity is part of the IPC triggering event, inhibition of IPC triggering stimuli might limit the use of cardioprotective Gap peptides.
Characterization of Two-Component System CitB Family in Salmonella Pullorum
Authors: Kang Xiamei , Zhou Xiao , Tang Yanting , Jiang Zhijie , Chen Jiaqi , Mohsin Muhammad , Yue Min
Published: September 2022

Salmonella enterica, serovar Gallinarum, biovar Pullorum, is an avian-specific pathogen which has caused considerable economic losses to the poultry industry worldwide. Two-component systems (TCSs) play an essential role in obtaining nutrients, detecting the presence of neighboring bacteria and regulating the expression of virulence factors. The genome analysis of S. Pullorum strain S06004 suggesting the carriage of 22 pairs of TCSs, which belong to five families named CitB, OmpR, NarL, Chemotaxis and LuxR. In the CitB family, three pairs of TCSs, namely CitA-CitB, DcuS-DcuR and DpiB-DpiA, remain unaddressed in S. Pullorum. To systematically investigate the function of the CitB family in S. Pullorum, four mutants, ΔcitAB (abbreviated as Δcit), ΔdcuSR (Δdcu), ΔdpiBA (Δdpi) and ΔcitABΔdcuSRΔdpiBA (Δ3), were made using the CRISPR/Cas9 system. The results demonstrated that the CitB family did not affect the growth of bacteria, the results of biochemical tests, invasion and proliferation in chicken macrophage HD-11 cells and the expression of fimbrial protein. But the mutants showed thicker biofilm formation, higher resistance to antimicrobial agents, enhanced tolerance to inhibition by egg albumen and increased virulence in chicken embryos. Moreover, the deletion of Dpi TCS was detrimental to survival after exposure to hyperosmotic and oxidative environments, as well as the long-term colonization of the small intestine of chickens. Collectively, we provided new knowledge regarding the possible role of the CitB family involved in the pathogenic processes of S. Pullorum.
Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of CaPAO2 and CaPAO4 in the Cold Tolerance of Pepper (Capsicum annuum L.)
Authors: Zhang Jianwei , Liang Le , Xiao Jiachang , Xie Yongdong , Zhu Li , Xue Xinru , Xu Linyu , Zhou Peihan , Ran Jianzhao , Huang Zhi , Sun Guochao , Lai Yunsong , Sun Bo , Tang Yi , Li Huanxiu
Published: September 2022

Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H2O2). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named CaPAO1–CaPAO6 according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). CaPAO2, CaPAO4, and CaPAO6 were ubiquitously and highly expressed in all tissues, CaPAO1 was mainly expressed in flowers, whereas CaPAO3 and CaPAO5 were expressed at very low levels in all tissues. RNA-seq analysis revealed that CaPAO2 and CaPAO4 were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. CaPAO2 and CaPAO4 overexpression in Arabidopsis thaliana significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H2O2, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (AtCOR15A, AtRD29A, AtCOR47, and AtKIN1). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars.
Down-Regulation of Double C2 Domain Alpha Promotes the Formation of Hyperplastic Nerve Fibers in Aganglionic Segments of Hirschsprung’s Disease
Authors: Xiao Jun , Meng Xinyao , Chen Ke , Wang Jing , Wu Luyao , Chen Yingjian , Yu Xiaosi , Feng Jiexiong , Li Zhi
Published: September 2022

Hirschsprung’s disease (HSCR) is a common developmental anomaly of the gastrointestinal tract in children. The most significant characteristics of aganglionic segments in HSCR are hyperplastic extrinsic nerve fibers and the absence of endogenous ganglion plexus. Double C2 domain alpha (DOC2A) is mainly located in the nucleus and is involved in Ca2+-dependent neurotransmitter release. The loss function of DOC2A influences postsynaptic protein synthesis, dendrite morphology, postsynaptic receptor density and synaptic plasticity. It is still unknown why hyperplastic extrinsic nerve fibers grow into aganglionic segments in HSCR. We detected the expression of DOC2A in HSCR aganglionic segment colons and established three DOC2A-knockdown models in the Neuro-2a cell line, neural spheres and zebrafish separately. First, we detected the protein and mRNA expression of DOC2A and found that DOC2A was negatively correlated with AChE+ grades. Second, in the Neuro-2a cell lines, we found that the amount of neurite outgrowth and mean area per cell were significantly increased, which suggested that the inhibition of DOC2A promotes nerve fiber formation and the neuron’s polarity. In the neural spheres, we found that the DOC2A knockdown was manifested by a more obvious connection of nerve fibers in neural spheres. Then, we knocked down Doc2a in zebrafish and found that the down-regulation of Doc2a accelerates the formation of hyperplastic nerve fibers in aganglionic segments in zebrafish. Finally, we detected the expression of MUNC13-2 (UNC13B), which was obviously up-regulated in Grade3/4 (lower DOC2A expression) compared with Grade1/2 (higher DOC2A expression) in the circular muscle layer and longitudinal muscle layer. The expression of UNC13B was up-regulated with the knocking down of DOC2A, and there were protein interactions between DOC2A and UNC13B. The down-regulation of DOC2A may be an important factor leading to hyperplastic nerve fibers in aganglionic segments of HSCR. UNC13B seems to be a downstream molecule to DOC2A, which may participate in the spasm of aganglionic segments of HSCR patient colons.
Developing an Online Dashboard to Visualize Performance Data—Tennessee Newborn Screening Experience
Authors: Lechner Charles , Rumpler Marc , Dorley Mary Christine , Li Yinmei , Ingram Amanda , Fryman Hilary
Published: September 2022

Newborn screening (NBS) is a vital public health program and delays in the screening process can lead to catastrophic outcomes for infants and their families. Efforts to improve screening quality in Tennessee are proactive and ongoing. From these efforts, an open-access dashboard has been developed to address a need for methods to better visualize performance data, promote data transparency, and drive quality improvement. Dashboard development was a collaboration between a fellow from the Association of Public Health Laboratories (APHL) and Tennessee NBS staff. Iterative dashboard prototypes were developed using Tableau software and incorporated feedback from Tennessee birthing facility staff and health experts. Infrastructure and procedures were created to reduce the burden of future dashboards. Eight NBS performance indicators are visualized across several views. These views are designed to provide an overview of NBS performance data when first accessed, then allow for a drill-down into specific data. This dashboard drives introspection at the state and facility level, making it possible to identify potential issues and necessary corrective actions earlier, therefore improving the completeness and timeliness of NBS in Tennessee. The experiences from developing this dashboard can be applied to future dashboard development in Tennessee NBS and other public health programs implementing similar measures.
Factors That Affect the Usage Intention of Virtual Learning Objects by College Students
Authors: Gaviria Diana , Arango Juan , Valencia-Arias Alejandro , Palacios-Moya Lucia , Velez Holguin Rosa , Gallegos Ruiz Ada Lucia
Published: September 2022

In recent years, the introduction of Information and Communications Technologies (ICTs) into several aspects of daily life has become more relevant, particularly in higher education. This phenomenon has resulted in a digital integration that involves the infrastructure of higher education institutions as well as the materials used in the teaching process. Therefore, a study was conducted to investigate the factors that determine usage intention of Virtual Learning Objects (VLOs) by college students in finance and accounting programs in the city of Medellín. This quantitative research employed 86 self-administered questionnaires that enabled us to identify a positive association between attitude toward usage, subjective norms, and the intention to employ this type of content as a learning strategy. In conclusion, models like this need to be developed to understand the acceptance of new technologies in learning processes from different perspectives, and even more so considering that most of the existing literature regarding the subject has been focused on qualitative studies.
The Crystal Structure of Carbonic Acid
Authors: Benz Sebastian , Chen Da , Möller Andreas , Hofmann Michael , Schnieders David , Dronskowski Richard
Published: September 2022

Ubiquitous carbonic acid, H2CO3, a key molecule in biochemistry, geochemistry, and also extraterrestrial chemistry, is known from a plethora of physicochemical studies. Its crystal structure has now been determined from neutron-diffraction data on a deuterated sample in a specially built hybrid clamped cell. At 1.85 GPa, D2CO3 crystallizes in the monoclinic space group P21/c with a = 5.392(2), b = 6.661(4), c = 5.690(1) Å, β = 92.66(3)°, Z = 4, with one symmetry-inequivalent anti-anti shaped D2CO3 molecule forming dimers, as previously predicted. Quantum chemistry evidences π bonding within the CO3 molecular core, very strong hydrogen bonding between the molecules, and a massive influence of the crystal field on all bonds; phonon calculations emphasize the locality of the vibrations, being rather insensitive to the extended structure.
Mass Trapping and Larval Source Management for Mosquito Elimination on Small Maldivian Islands
Authors: Jahir Akib , Kahamba Najat F. , Knols Tom O. , Jackson Gordon , Patty Nila F. A. , Shivdasani Sonu , Okumu Fredros O. , Knols Bart G. J.
Published: September 2022

Globally, environmental impacts and insecticide resistance are forcing pest control organizations to adopt eco-friendly and insecticide-free alternatives to reduce the risk of mosquito-borne diseases, which affect millions of people, such as dengue, chikungunya or Zika virus. We used, for the first time, a combination of human odor-baited mosquito traps (at 6.0 traps/ha), oviposition traps (7.2 traps/ha) and larval source management (LSM) to practically eliminate populations of the Asian tiger mosquito Aedes albopictus (peak suppression 93.0% (95% CI 91.7–94.4)) and the Southern house mosquito Culex quinquefasciatus (peak suppression 98.3% (95% CI 97.0–99.5)) from a Maldivian island (size: 41.4 ha) within a year and thereafter observed a similar collapse of populations on a second island (size 49.0 ha; trap densities 4.1/ha and 8.2/ha for both trap types, respectively). On a third island (1.6 ha in size), we increased the human odor-baited trap density to 6.3/ha and then to 18.8/ha (combined with LSM but without oviposition traps), after which the Aedes mosquito population was eliminated within 2 months. Such suppression levels eliminate the risk of arboviral disease transmission for local communities and safeguard tourism, a vital economic resource for small island developing states. Terminating intense insecticide use (through fogging) benefits human and environmental health and restores insect biodiversity, coral reefs and marine life in these small and fragile island ecosystems. Moreover, trapping poses a convincing alternative to chemical control and reaches impact levels comparable to contemporary genetic control strategies. This can benefit numerous communities and provide livelihood options in small tropical islands around the world where mosquitoes pose both a nuisance and disease threat.
Interaction between Microplastics and Pharmaceuticals Depending on the Composition of Aquatic Environment
Authors: Klavins Maris , Klavins Linards , Stabnikova Olena , Stabnikov Viktor , Marynin Andrii , Ansone-Bertina Linda , Mezulis Marcis , Vaseashta Ashok
Published: September 2022

A large amount of the globally produced plastics are not treated and are eventually released into landfills or natural environments, including surface waters. The plastics that enter the aquatic environment are very often microplastics, which are produced in households by the slow degradation or abrasion of plastic products, or as whole plastic products, which eventually degrade (abrasion, photodegradation). Together with microplastics, other pollutants such as pharmaceuticals of various kinds enter surface waters—both of these counterparts can interact with each other as well as with organic and inorganic molecules available in the natural environment. The aim of this study was to identify the interaction of microplastics with pharmaceuticals, especially under conditions that are common in inland waters as well as the seas and oceans that the rivers feed their water into. It was found that salinity has a great impact on the sorption capacity of microplastics and pharmaceuticals. The sorption of naturally occurring humic substances (humic and fulvic acids) can greatly increase when the microplastic–pharmaceutical complex is formed; however, the priority of the interaction happens with pharmaceuticals and humic substances. Such complexes can influence the organisms that feed on small organic-matter particles, as they can be mistaken for food and thus be transferred throughout the food chain.
Assessing Microplastic Prevalence and Dispersion from Saigon Urban Canals via Can Gio Mangrove Reserve to East Sea by Raman Scattering Microscopy
Authors: Khuyen Vo , Le Dinh , Le Hung , Fischer Axel , Dornack Christina
Published: September 2022

Plastic pollution is one of the significant environmental concerns due to the threefold increase in global plastic waste. Marine microplastics, including petroleum-based plastic pieces and synthetic and artificial fibers smaller than 5 mm, are not only ubiquitous in natural water but also high in wastewater streams due to the direct discharge, transfer and breakdown of plastic items. This research aims to investigate the presence and dispersion of microplastics in the downtown area and coastal suburban area of Ho-Chi-Minh City by using Raman microscopy. As a result, the most common plastics (PE, PET, PA, PP, PVC, PS and PMMA) were detected, and most of them were fibrous shorter than 500 μm. The total microplastics decreased gradually from the urban waterborne (up to 220 MPs/L) via Can Gio UNESCO Mangrove Biosphere Reserve (10 MPs/L) and to the East Sea (3 MPs/L), which reveals the potential role of the mangrove in reducing marine contaminants including microplastics. This study provides important insights into microplastic pollution in the Western Pacific Region, especially the Saigon-Dong Nai river systems, supporting useful data for natural water resources management.
Pobreza: consideraciones sobre la cobertura periodística en medios de comunicación social
Authors: Quiñónez Gómez Herly Alejandra
Published: September 2022

La medición de la pobreza ha sido investigada principalmente desde el enfoque unidimensional que estudia los aspectos económicos. Sin embargo, desde 2010 cobra auge el enfoque multidimensional, el cual incluye conceptos y mediciones relacionados con el bienestar individual y familiar y la posibilidad de diagnosticar necesidades para ser atendidas mediante políticas públicas. Por eso, es relevante describir cómo es la cobertura periodística de la pobreza en los medios de comunicación social, mediante la caracterización de las publicaciones académicas referidas al tema. Esta investigación analiza los documentos hallados en los sistemas de información Dialnet Plus y Scielo desde 2008 hasta 2020. Con el análisis de contenido de seis categorías, se obtienen los siguientes resultados: existe negación, minimización, sensacionalismo, amarillismo y espectacularidad en los medios de comunicación social; la pobreza es un tema esporádico porque aparece relacionada a eventos naturales y la violencia; la agenda periodística está referida a la inflación y la canasta básica; el género periodístico predominante es la noticia y existe una imagen estereotipada y descontextualizada para identificar a personas en condición de pobreza. Se concluye que están emergiendo estudios de la cobertura periodística con enfoque multidimensional.
Análisis de las estrategias instruccionales utilizadas para el aprendizaje significativo de la definición de derivada de funciones de una variable
Authors: Manjarrez Pontón Cesar Alfonso , Romero Rincón Yaritza Josefina
Published: September 2022

La presente propuesta espera introducir un cambio que trasciende el enfoque algorítmico generalmente que se tiene de la derivada, se busca la construcción del conocimiento fundamentado en su aprendizaje significativo, a partir de su concepción geométrica y funcional, como razón de cambio. Se ha plantado como objetivo, analizar las estrategias instruccionales utilizadas para el aprendizaje significativo de la definición de derivada de funciones de una variable. Basada en las teorías de Barriga, Cabero, Romero, Vera y González. Enmarcada dentro del paradigma positivista, con enfoque cuantitativo, de investigación descriptiva, de campo, no experimental y transeccional. La muestra fue estratificada, de sesenta y cuatro (64) alumnos y población censal de seis (6) docentes de la asignatura Cálculo I, a los que se les administró un cuestionario de treinta tres (33) reactivos, versionado para los estudiantes. Se concluyó, que las estrategias más trabajadas son las preinstruccionales y las menos, las coinstruccionales. Asimismo, las estrategias más empleadas por los docentes y los estudiantes, son los objetivos, y la menos empleada por los docentes son los organizadores previos y por los estudiantes, la resolución de problemas. Por lo tanto, se deben implementar mejores formas para aprender, tales como mayor capacidad de visualización de las funciones matemáticas, flexibilidad para aprender de manera individualizada atendiendo al ritmo propio de cada estudiante, acercamiento a la interacción grupal e intercambio de saberes de manera presencial y optimizarlas de manera virtual, empleo de herramientas web para la resolución de problemas matemáticos con implicaciones en la vida cotidiana.
Perception of non-stationary wind noise in vehicles
Authors: Carr Daniel , Davies Patricia
Published: September 2022

Predictors of people's responses to noise inside cars are used by car companies to identify and address potential noise problems. Because significant advances have been made in the reduction of engine, powertrain, and tire/road noise, it is now important to pursue reductions in wind
or aerodynamic noise. While models of loudness are commonly used to predict people's responses to stationary wind noise, some wind noises are less acceptable than is predicted by the loudness metric. Additional sound characteristics may account for this. Two listening studies were designed
to examine the usefulness of including sound quality metrics in addition to loudness and sharpness in models used to predict acceptability for non-stationary wind-noise sounds, particularly noise with the kind of variations that are expected from wind gusts. Test sounds were based on recordings
made in cars in a wind tunnel. A simulation method was implemented to generate sounds with controlled gusting features by modifying stationary noise recordings. A gusting metric was designed to predict subjects' acceptability responses based on the strength, modulation rate, and duration of
the gusts.
Diagnóstico de la ansiedad personal del adolescente en las actividades educativas
Authors: Aminova D.K. , Skoblikova T.V. , Latipov Z.A. , Listik E.M. , Kurganova E.A.
Published: September 2022

The paper provides a theoretical and analytical review of modern methods of diagnosing anxiety states and personal anxiety, which have been used by Russian scientists in recent years. The paper presents the arguments of scientists about the nature and psychological and pedagogical characteristics of personal anxiety as a property of personality, mental state and character traits. The paper describes the scientific apparatus and methodological tools of diagnostic procedures. The methods used in the study (observation, survey, analysis of the products of educational activity, Spielberg-Khanin’s method of personal and situational anxiety, the J. Taylor’s method of measuring the level of anxiety, the method “Multidimensional assessment of child anxiety” (MACA), statistical methods of data processing are highlighted and the empirical results are discussed. The results  indicat that it is necessary to separate the scientific concepts of anxiety and uneasiness as non-fundamental and having a different psychological and physiological basis.
Pendidikan Kebangsaan dalam Perspektif Al - Qur'an
Authors: Laila Azzah Nor
Published: September 2022

Problems of disunity and less of public awareness about the sense of belonging in the nation, often become a trigger for tension and disharmony in social relations. Both within the scope of fellow religious people, as well as within a nation. To mediate this, the planting of a sense of belonging to the nation or education about nationality to the community is important as a solution. This paper aims to find the message of national education in the Qur'an. Al-Qur'an as a Muslim holy book is full of educational values. This paper uses thematic methods and content analysis of the text of the Qur'anic verses about the nation. The findings of national education in the perspective of the Qur'an are recommendations to uphold the equal rights of citizens in a nation. The importance of government cooperation with its people. The peace and tranquility of a country is essentially based on the attitude of its inhabitants, it is carried out by means of gratitude, use of voting rights, deliberation, courtesy and honesty. The importance of introspection and evaluation. Every citizen of the country, both the people and the authorities, must have a mistake. All residents should be aware and admit their mistakes, then correct and apologize to those who are judged and to the Forgiving.
Pokok-pokok Ajaran Aswaja Menurut KH. Ma'ruf Irsyad Kudus
Authors: Rohman Fathur
Published: September 2022

KH. Ma'ruf Irsyad is one of the Kiai from Kudus city who is very consistent and eager in fighting for the teachings of Ahl al-Sunnah wa al-Jama'ah. Besides being active in the PCNU management of Kudus Regency, he also actively campaigned aswaja teachings through madrasa and majlis ta'lim. According to him, Aswaja is the best way to carry out Islamic teachings because Aswaja was pioneered by the Salaf scholars whose scientific sanad continued to the Prophet and his companions. Kiai Ma'ruf refused propaganda “back to the Qur'an and Hadith” because digging Islamic teachings from its immediate source is not an easy matter that can be done by just anyone. Without the tools of science and high integrity, exploring Islamic teachings directly from the source will actually lead to being lost and misleading. Therefore, the safest way to practice Islam is by following the salaf scholars. This article discusses the important points of the teachings of Ahl al-Sunnah wa al-Jama'ah according to KH. Ma'ruf Irsyad Kudus. There are nine points presented by KH. Ma'ruf Irsyad about the Aswaja teachings, namely: using mizan al-syar'i, consistent with the truth, maintaining unity, following the salaf scholars, prioritizing the Shari'a rather than reason, the Shari'ah as a measure of good and bad,  taqwa in all conditions, benefiting humans, doing sincerely.
Etika Sabar Dalam Berdakwah Perspektif Syaikh Ali Mahfudz
Authors: Maun Maun
Published: September 2022

AbstrakPenyeru agama dalam menyampaikan pesannya sudah menjadi ketentuan Allah bahwa ia akan dicoba dengan dihadapkan beberapa ujian seperti hujatan, pengolok-olok bahkan kekerasan yang tidak lain sesuai dengan kemampuan hambanya. Semua itu merupakan ujian dan siapa yang  dapat melaluinya dengan kesabaran (lapang dada) maka ia akan mendapatkan imbalan oleh karena itu sabar merupakan sifat yang harus dimiliki oleh siapapun, sebab dengan kesabaran seorang hamba mampu menguatkan diri dalam menghadapi cobaan apapun dalam ajarann islam sifat sabar merupakan akhlak yang mulia, seorang da’i harus memiliki sifat yang terpuji itu untuk dijadikan sebuah benteng dalam perjalann dakwahnya, dengan demikian penelitian ini untuk mengetahui Pemikiran Syekh Ali Mahfudz dalam kitab Hidayat al Mursyidin tentang sifat Sabar bagi seorang da’i. Jenis penelitian yang dilakukan adalah studi pustaka (library research) dengan metode analisis deskriptif menurut Syekh Ali Mahfudz bahwa salah satu kunci kesuksesan dalam berdakwa adalah seorang da’i harus memiliki sifat sabar sebagaiman a yang telah dimiliki Rasul ketika berdakwahKata kunciSabar, da’i, pemikiran syekh Ali Mahfudz AbstractThe caller of religion in conveying his message has become God's provision that he will be tried by being faced with several tests such as blasphemy, ridicule and even violence that are none other than the ability of his servant. All of this is a test and whoever can go through it with patience, he will get a reward, therefore patience is a trait that must be possessed by anyone, because with patience a servant is able to strengthen himself in the face of any trial in Islamic teachings. noble character, a preacher must have a commendable nature to be used as a fortress in his da'wah journey, thus this research is to find out the thoughts of Sheikh Ali Mahfudz in the book Hidayat al Mursyidin about the nature of patience for a preacher. The type of research carried out is library research with descriptive analysis methods according to Sheikh AliMahfudz that one of the keys to success in preaching is that a preacher must have a patient nature as the Apostle had when preaching.KeywordsPatience, da'i, the thoughts of Sheikh Ali Mahfudz
Penanaman Nilai-Nilai Pendidikan Ahlussunnah Wal Jamaah An-Nahdliyyah di SMP Islam Pecangaan Jepara
Authors: Fikar Sirojul , Saefudin Ahmad
Published: September 2022

AbstrakPenelitian ini bertujuan untuk menggambarkan peran guru PAI dalam menanamkan nilai-nilai pendidikan ahlussunnah wal jamaah (Aswaja) di SMP Islam Pecangaan. Selain itu, juga untuk  mengidentifikasi faktor pendukung dan penghambatnya. Penelitian kualitatif berjenis field research ini menggunakan teknik observasi, wawancara, dan dokumentasi dalam pengumpulan data. Hasil penelitian menunjukkan bahwa nilai ahlussunah wal jamaah An-Nahdliyah yang ditanamkan oleh guru PAI kepada siswa meliputi tawassuth, tawazzun, tasamuh dan i'tidal. Sebagai konservator, guru PAI berperan memberikan arahan berupa materi terkait dengan moderasi Islam yang bisa dilaukan dalam kehidupan sehari-hari seperti toleransi, berbuat baik kepada sesama, dan menghormati pendapat orang lain. Guru PAI juga selaku inovator yang memiliki ide-ide baru dalam menanamkan nilai ahlussunah wal jamaah An-Nahdliyah. Selaku transmitor, guru PAI memanfaatkan metode ceramah dan dakwah untuk menanamkan nilai ahlussunah wal jamaah An-Nahdliyah. Aspek latar belakang pendidikan guru PAI menjadi faktor pendukung penanaman nilai Aswaja An-Nahdliyyah, di samping juga faktor kedewasan berpikir siswa. Fasilitas dan lingkungan sekolah juga menambah daya dukung penanaman nilai-nilai tersebut. Pada sisi yang lain, adanya guru yang linearitas kesarjanaannya kurang sesuai menjadi faktor penghambat penanaman nilai Aswaja An-Nahdliyyah.
Development of an Algorithm to Analyze Vacancies in the Labor Market Based on Open-Source Data
Authors: Khokhlova O. A. , Khokhlova A. N. , Choyzhalsanova A. T.
Published: September 2022

In the introductory part of the article, the authors substantiate the relevance of developing methodological tools for analyzing job vacancies in the labor market in the context of the modern technological revolution, which significantly increases requirements for professional knowledge and experience of working personnel and changes the ratio between traditional and new professions.To assess the current situation on the labor market and the demand for currently existing professions, the main section of the published results of the study presents the algorithm for analyzing vacancies using large data arrays from open sources using mathematical and statistical tools and machine learning methods using the Python programming language and the IBM SPSS modeler analytical platform. The algorithm includes: parsing data on vacancies, analyzing vacancies by the main criteria, clustering vacancies by salary level and building a neural network model – a multilayer perceptron of the dependence of salary on a number of predictors. It should be noted that the developed algorithm is universal, because it can be used to analyze big data from any open source at a certain point in time.The results of the analysis will allow researchers and specialists of management structures to more realistically assess the current situation on the labor market, educational institutions will be able to adjust training programs in accordance with the modern requirements of employers, employers will make decisions on the development of competencies in their field of activity and conduct a comparative analysis of demanded vacancies in terms of quantitative and qualitative characteristics, and for the applicant it will be easier to see the demand for vacancies in the labor market and develop new skills.
Approaches to Assessing Regional Differentiation of Mortality from Coronavirus
Authors: Kuchmaeva O. V. , Kalmykova N. M. , Kolotusha A. V.
Published: September 2022

The study aims to substantiate statistical and methodological approaches that allow the most accurate measurement of the contribution of the COVID-19 pandemic to the level and dynamics of the total mortality in the Russian Federation (in 2019–2020), and to assess the regional differentiation of mortality from the pandemic. The relevance of the study stems from revealing the role of some factors in the rise of mortality rates in Russia (according to data for 2020).In the study, the authors used regression analysis with a set of factors determining pandemic-induced differences in changes in mortality rates across regions of the Russian Federation as a basic analytical tool. The information base of the study is a set of official statistics data, as well as regional representative results of sample socio-demographic surveys of Rosstat.The authors used a set of regression models to test the hypotheses about the influence of a combination of demographic and socioeconomic factors on the increase in the total mortality rate. A repeating set of factors affecting the increase in mortality in different models may indicate the stability of the influence of the following factors: the share of people employed in the service sector, migration turnover, the presence in a region of a city of 500 thou. inhabitants or more, and the death rate from COVID-19. The set of factors influencing the increase in mortality differs by type of settlement and by gender.The article argues for the possibility of using the crude mortality rate as a dependent variable in assessing the causes of mortality growth. A significant part of the regional variation in the increase in the total mortality rate in 2020 in Russian regions is explained by the characteristics of the demographic structure of the region (the share of elderly, the size of the household, the share of people with cancer), as well as the high population density and frequency of social contacts (especially in cities). Rise of COVID-19 related mortality rate had impact on the increase of the total urban mortality rate but did not lead to any significant growth in rural mortality rate.The problem of the need to improve the information base for the selection of objective indicators and methods for analyzing the contribution of the COVID-19 epidemic to the level and dynamics of mortality is emphasized. An important methodological conclusion relevant for further research is the need to search for instrumental variables for COVID-19 related mortality indicators, due to the correlation of factors with the total mortality rate and with the mortality rate from COVID-19, as well as the need for further analysis of changes in the national health care system and its funding amid the pandemic.
Statistical Modeling of Regional Labor Productivity
Authors: Burtseva T. A. , Frenkel A. A. , Surkov A. A.
Published: September 2022

The article from the perspective of interregional comparative analysis methodology substantiates the system of labor productivity factors and presents the results of statistical modeling of labor productivity in the regions of Russia in 2020.The shortcomings of the existing measures of labor productivity in international and domestic practice are noted, namely, the ambiguity and inconsistency of the assessment results. The authors propose a technique for constructing an integral index of regional labor productivity and substantiate a four-factor econometric model of the dependence of the integral index of regional labor productivity, which allowed for an improved system of labour productivity factors to be applied in regional economic analysis. Estimates are given of the relative impact of both traditional and new factors of regional labor productivity, which are the increasing incidence rate and the growing share of unprofitable enterprises (organizations), investments and industrial output per capita in the regions of Russia in 2020.The need to monitor regional labor productivity factors annually to help bring forward problems in the regions is justified. The reasons that led to the insufficient level of labour productivity (compared to Russian average) were identified. According to the authors (following the results of socio-economic development in 2020), they should include low growth rates of investment and production volumes, as well as an increase in the incidence and in the share of unprofitable enterprises and organizations.
The Analysis of Consumer Demand in Russia: Two-Stage Construction of Analytical Indexes
Authors: Gorbunov V. K. , Lvov A. G.
Published: September 2022

The article develops methods for constructing analytical («economic») indices, and their application in the analysis of consumer demand in Russia based on official statistics on the consumption of goods and services (468 items for the period 2012–2017). The «economic» direction of indexology, which is based on the use of consumer demand theory, when constructing prices indices and measuring consumption dynamics, takes into account consumer preferences instead of the subjective biases of statisticians or authors of various index formulas. This direction has a long history, dating back to the 1924 work of the Soviet economist A.A. Konüs. The development of this concept in the works of Western researchers refers only to individual or household demand due to the absence of market demand theory in neoclassical Economics. Such a holistic theory of market demand was developed in recent years in the works of V.K. Gorbunov, and on this basis were developed methods for constructing analytical indices, using the ambiguity of restoring the utility function from a finite set of data for the variant determination of indices with characteristics, namely optimistic, pessimistic, and objective.To compare the traditional and «economic» directions of indexology, the authors constructed Laspeyres, Paasche and Fischer price and quantity indices. The indices were calculated both for the summary statistics of prices and consumption of the population, and for the main groups of consumer goods: food products, non-food products and services. General (two-stage) indices of all types were constructed for group indices and the consistency in aggregation property was tested. The obtained results are a new example of a successful verification of the holistic market demand theory.
Florística e fitossociologia de uma área de caatinga no munícipio de Jardim de Piranhas - RN, Brasil
Authors: De Andrade Rênio Leite , Rolim Emili Caroline de Abreu , Tavares Jean Leite , do Nascimento José Wilton Ferreira , Sousa Rui Santiago , Da Silva Girleno Pereira , Medeiros Luiz Fernandes da Silva , Sales Francisco das Chagas Vieira
Published: September 2022

Este trabalho teve como objetivo caracterizar a composição florística e fitossociológica de um fragmento de caatinga, localizado no município de Jardim de Piranhas, Rio Grande do Norte. Realizou-se uma amostragem inteiramente aleatória, a partir de 15 unidades amostrais de 20 x 20 (400 m²). Foi inventariado para cada unidade amostral, todos os indivíduos florestais arbustivo-arbóreos vivos com circunferência da altura do peito (CAP ≥ 6 cm) mensurados a 1,30 m do solo, registrando-se a circunferência, altura total e nome comum por indivíduo. Avaliou-se a composição florística, diversidade e as estruturas vertical e horizontal. Foram amostrados 833 indivíduos distribuídos em onze espécies e seis famílias, sendo a família Fabaceae a mais representativa, com o total de cinco espécies, enquanto as demais famílias apresentaram apenas uma espécie cada. Já as espécies com maior valor de importância foram a Mimosa tenuiflora (Willd.) Poir. e Aspidosperma pyrifolium Mart & Zucc., responsáveis por 67,11% dos indivíduos amostrados. Assim, a diversidade de espécie encontrada no fragmento através dos índices estudados foi considerada fora do padrão normal em áreas de Caatinga, apresentando baixa riqueza e diversidade, com apenas nove espécies, grande heterogeneidade ambiental e baixa dominância ecológica.
PENINGKATAN AKTIVITAS BELAJAR SISWA DALAM MATA PELAJARAN SENI BUDAYA MELALUI MODEL PEMBELAJARAN KOOPERATIF TIPE QUANTUM LEARNING DI KELAS VII SMPN 13 PADANG
Authors:   SMPN 13 PADANG, ermayetti ermayetti
Published: September 2022

Based on the learning activities of students of Class VII SMPN 13 Padang in Arts subjects Budayaditemukan that the learning activities of students in the subjects of art Budayamasih very low. The purpose of this study is to describe and obtain information about the Enhanced Learning Activities Arts students in subjects Budayamelalui cooperative learning model Quantum Learning in Class VII SMPN 13 Padang of West Sumatra Province. This research is a class act. The procedure in this research include planning, action, observation and reflection. The study consisted of two cycles of the four meetings. Subject of the study consisted of 32 students of class VII SMPN 13 Padang. Data were collected by using observation sheet. Data were analyzed using percentages. Based on the results of research and discussion that has been stated in the previous chapter, it can be concluded that it can be concluded that the model pembelajaan demonstration can improve students' learning activities in the subjects of Arts and Culture in SMPN 13 Padang. Activities of students in the first cycle was 71.71 (enough) increased to 76.63 (good) with an increase of 9.82.
FORECASTING OYSTER MUSHROOM SALES USING THE DOUBLE EXPONENTIAL SMOOTHING METHOD AT KUB RUMAH MUSHROOM BERSAMA
Authors: Wulandari Winda , Syafwan Havid , Ihsan Muhammad
Published: September 2022

The amount of oyster mushroom production is often not by consumer orders due to a lack of attention to the mushroom stock in the Mushroom House KUB. The purpose of this study is to apply double exponential smoothing (DES) to predict future sales of oyster mushrooms so that consumer needs are met. This research method uses quantitative methods. The data source comes from data on sales of oyster mushrooms from June 2021-May to 2022. The data is analyzed using double exponential smoothing based on alpha and MAPE values. The results showed that an alpha of 0.6 with a MAPE value of 6.23% was the alpha with the smallest MAPE. This study concluded that the double exponential smoothing method could accurately predict future oyster mushroom sales.
ON-SR UII: AN ONLINE SELF-REGULATED LEARNING WEB APPLICATION TO ASSIST INDEPENDENT COLLEGE LEARNERS
Authors: Pratama Ahmad R. , Rahayu Puji , Setiyadi Andri , Azhar M. Fachry , Ashshiddiq M. Fajri
Published: September 2022

Self-regulated Learning (SRL) is a learning method that puts a strong emphasis on the importance of self-learning skills. Unfortunately, many existing educational technologies employed by colleges and universities continue to place a premium on technical support for the learning process within the classroom that does not provide the same level of support for SRL. This study aims to close this gap by developing the ON-SR UII, a new SRL platform that can assist college students in their quest to become independent learners. Using the design thinking approach, ON-SR UII is developed as a responsive web app that can be accessed by college students through the Internet anywhere at any time at their own pace using any computing device of varying screen sizes. This article describes the process by which ON-SR UII was designed prior to its first prototype being developed, deployed, and evaluated by stakeholders for functionality, usability, and responsiveness. The encouraging results indicate that ON-SR UII has the potential to be widely implemented, allowing for the measurement of its implications in future research.