METHODOLOGY FOR STUDYING INTERACTIONS BETWEEN THE MICROBIOCENOSIS OF THE HUMAN ORAL CAVITY AND WHOLE ORGANISM
Authors: Zaitsev A.V. , Boychenko O.M. , Kotelevskaya N.V. , Nikolishyn A.K. 
Published: September 2022

Stable oral microbiom is associated with the occurrence of dental caries and diseases of periodontal tissues and oral mucosa. Of all the species, streptococci, lactobacilli, actinomycetes and fungi of the genus Candida are found as demonstrating the highest activity. The pathogenicity of a microbial agent may depend on its biological properties. It can also be regarded as an integrated result of the action between several symbionts within the biocenosis or depends on the interaction between the symbionts of the oral biocenosis and the host organism, and this aspect is highlighted in this article. The aim of this study is to apply data from the analysis of the relationship between the host organism and microorganisms, which are known as cariogenic factors, for applying in medical, research and doctor training practice. We analyze the relationship between the host organism and gariogenic microorganisms based on the data obtained from studies of young people with different DMF indices, conducted at the Poltava State Medical University. The paper considers the relationship between the host organism and the oral microbiota in different states of dental caries. The analysis mainly focused on lactobacilli, γ-hemolytic streptococci (which include S. mutans), fungi of the genus Candida, and actinomycetes. The study showed that the number of people with γ-hemolytic streptococci is one hundred percent. This dependence can be expressed by the formula y=100. The number of people with lactobacilli varies from 43% with DMF=0 and over 70% for DMF≥6. This dependence can be expressed by the formula y=13.5x+29. These dependencies change according to the linear laws. The number of people, carriers of actinomycetes, by DMF=0 is also equal to zero. But with an increase in the intensity of caries, the percentage of carriers of these representatives of microflora does not increase according to the linear laws. The formula for such changes is: y=12.5x2-32.5x+20. The percentage of carriers of Candida fungi changes according to the formula: y=5x2-10x+5. These dependences change not according to the linear, but according to the square laws. That is, such changes have a critical point, so-called the bifurcation point. From this point, the relationship between an individual and the indicated representatives of the oral microbiocenosis becomes unstable. Uncertainty arises regarding their further interaction: the "organism-microbiocenosis" system. It can become chaotic or reach a new level of functioning. This research indicates that the relationship between human organisms caring the above mentioned pathogens and representatives of their oral microbiocenoses change differently depending on the state of the oral cavity. One of the indicators of this state is the DMF index. The study of the relationship between microbiocenoses and their carriers should be applied in further scientific work, as well as in teaching activities in teaching sections related to dental caries and periodontal disease.