bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Factors and Genetic Biomarkers of Multiple Primary Cancers in Esophageal Cancer Patients
Authors: Yang Pei-Wen , Lin Mei-Chun , Huang Pei-Ming , Wang Cheng-Ping , Chen Tseng-Cheng , Chen Chun-Nan , Tsai Mong-Hsun , Cheng Jason Chia-Hsien , Chuang Eric Y. , Hsieh Min-Shu , Lou Pei-Jen , Lee Jang-Ming
Published: January 2021

Esophageal cancer (EC) is a deadly cancer that frequently develops multiple primary cancers (MPCs). However, the risk biomarkers of MPC in EC have hardly been investigated. We retrospectively enrolled 920 subjects with primary EC and analyzed the possible risk factors as well as MPC single-nucleotide polymorphisms (SNPs) from blood DNA. A total of 184 subjects (20.0%) were confirmed to have MPC, 59 (32.8%) had synchronous MPC, and 128 (69.6%) had head and neck cancer. Elderly EC patients have an increased risk of having gastrointestinal cancer (Odds ratio, OR[95% CI]=6.70 [1.49–30.19], p=0.013) and a reduced risk of developing HNC (OR[95% CI]=0.44 [0.24–0.81], p=0.008). MPC risk was also associated with betel nut chewing (OR[95% CI]=1.63, 1.14–2.32], p=0.008), the A allele of ALDH2:rs671 (p=0.074 and 0.030 for GA and AA, respectively), the CC genotype in CISH:rs2239751 (OR[95% CI]=1.99 [1.2–3.32], p=0.008), and the G allele of ERCC5:rs17655 (p=0.001 and 0.090 for GC and CC, respectively). ADH1B:rs1229984 also correlated with MPC risk (p=0.117). Patients carrying four risk SNPs had a 40-fold risk of MPC (OR[95% CI]=40.25 [6.77–239.50], p<0.001) and a 12.57-fold risk of developing second primary cancer after EC (OR[95% CI]=12.57 [1.14–138.8], p=0.039) compared to those without any risk SNPs. In conclusion, hereditary variations in ALDH2, CISH, ERCC5, and ADH1B have great potential in predicting the incidence of MPC in EC patients. An extensive cancer screening program during clinical follow-up would be beneficial for patients with high MPC susceptibility.
Prognostic Value of CT Imaging-Based Tumor Volume in Patients With Non-Surgical Esophageal Squamous Cell Carcinoma
Authors: Kang Ning , Fang Yeying , Zhu Huijun , Shi Zhiling , Chen Liuyin , Lu YuShuang , Wang Housheng , Lu Jiamei , Liu Wenqi , Hu Kai
Published: January 2021

Background

The American Joint Committee on Cancer-Tumor (AJCC-T) staging system for esophageal carcinoma patients, which is based on the depth of tumor invasion, is not applicable in some cases. This study aims to assess the prognostic value of CT imaging-based tumor volume and its usefulness for T staging in patients with non-surgical esophageal squamous cell carcinoma (ESCC).

Methods

We retrospectively reviewed the records of 158 ESCC patients undergoing definitive (chemo) radiotherapy from two hospitals. Tumor volume based on the CT imaging was calculated using the formula: V = πabc / 6. Three cutoff points for tumor volume were obtained with the X-tile software. Overall survival (OS) was analyzed using the Kaplan–Meier method. The -2 log-likelihood ratio and Akaike Information Criterion (AIC) value were evaluated to compare the AJCC-T staging system with the proposed T staging method.

Results

The median tumor volume was 19.8 cm³ (range from 1.0 to 319.5 cm³). The three optimal cutoff points of tumor volume were 12.7, 22.8, and 51.9 cm³, and the patients were divided into four groups named as proposed T1–T4 stages. The 3-year OS rates in patients with proposed T1 to T4 stages were 67.9%, 30.6%, 21.3%, and 5.3%, respectively. The −2 log-likelihood ratios of the AJCC-T stage and proposed T stage were 1,068.060 and 1,047.418, respectively. The difference in the AIC value between the two T staging systems was 18.642.

Conclusion

CT imaging-based tumor volume was superior to the depth of tumor invasion for T staging in predicting the prognosis of non-surgical ESCC patient.
Novel Compound Heterozygous Mutations in Two Families With Bernard–Soulier Syndrome
Authors: Minkov Milen , Zeitlhofer Petra , Zoubek Andreas , Kager Leo , Panzer Simon , Haas Oskar A.
Published: January 2021

Background: Bernard–Soulier Syndrome (BSS) is a rare autosomal recessive bleeding disorder with large platelets and thrombocytopenia. It is caused by homozygous or compound heterozygous mutations in the GP1BA, GP1BB, or GP9 genes, which together encode the platelet surface receptor glycoprotein complex GPIb-IX-V.

Objectives: We report two novel heterozygous mutations in the GP1BA and the GP9 genes, respectively.

Patients/Methods: We analyzed the platelet glycoprotein expression by flow cytometry and screened the relevant genes for responsible mutations in two unrelated families.

Results: Flow cytometric analyses revealed the absence of CD42a (GPIX) and CD42b (GPIb) on the platelets in the two affected siblings of family 1 and a significantly reduced expression of CD42b (GPIb) in the patient of family 2. In the two siblings, we identified a known frameshift (c.1601_1602delAT) and a novel nonsense mutation (c.1036C>T) in the GP1BA gene that abrogated the production of GP1bα. In the other patient, we found a novel missense mutation (c.112T>C) that was co-inherited with a common one (c.182A>G) in the GP9 gene, respectively. All analyzed heterozygous carriers were asymptomatic and had a normal GPIb-IX-V expression.

Conclusions: The two novel GP1BA and GP9 mutations reported herein increment the number of causative genetic defects in BSS.
Artocarpus tonkinensis Extract Inhibits LPS-Triggered Inflammation Markers and Suppresses RANKL-Induced Osteoclastogenesis in RAW264.7
Authors: Orecchini Elena , Mondanelli Giada , Orabona Ciriana , Volpi Claudia , Adorisio Sabrina , Calvitti Mario , Thuy Trinh Thi , Delfino Domenico V. , Belladonna Maria Laura
Published: January 2021

Artocarpus tonkinensis (At) leaf decoction, a traditional remedy prepared in North Vietnam by the Hmong ethnic group, is a tea extract rich in bioactive compounds that may have therapeutic effects in arthritis and backache. Indeed, it has been demonstrated that At is able to inhibit Th17 lymphocytes development and to protect mice in an experimental model of collagen-induced arthritis. By resorting to macrophage in vitro models of inflammation and osteoclastogenesis, we showed that At extract significantly reduced nitric oxide synthase 2 (NOS2) activity and IL-6 production by RAW 264.7 murine cells. Moreover, At demonstrated an anti-osteoclastogenic effect, as revealed by complete inhibition of TRAP-positive osteoclast formation and decreased expression of key osteoclast-related genes. This At activity likely relies on the inhibition of RANK downstream signaling pathway, as the activation of non-receptor tyrosine kinase Src is reduced upon RANKL-At exposure. Protective effect of At against bone loss was also enlightened in vivo by collagen-induced arthritis (CIA) experiment demonstrating that, although paw edema was only weakly opposed by drinking At decoction, bone and cartilage were well preserved in CIA+At mice and joint tissue expressed decreased levels of osteoclast marker genes respect to CIA control group. Maesopsin 4-O-β-D-glucoside (i.e., TAT-2, one of the main decoction bioactive components) was capable to contrast NOS2 activity, IL-6 expression and osteoclast formation, too, albeit to a lesser extent when compared to At decoction. Overall, this study enlightens another At cell target, macrophages, beside Th17 lymphocytes, and suggests that the anti-arthritic beneficial effects of At decoction largely derives from its ability to counteract not only inflammation, but also osteoclastogenesis.
CSF3 Is a Potential Drug Target for the Treatment of COVID-19
Authors: Fang Chao , Mei Jie , Tian Huixiang , Liou Yu-Ligh , Rong Dingchao , Zhang Wei , Liao Qianjin , Wu Nayiyuan
Published: January 2021

Coronavirus Disease 2019 (COVID-19) is an acute respiratory infectious disease that appeared at the end of 2019. As of July 2020, the cumulative number of infections and deaths have exceeded 15 million and 630,000, respectively. And new cases are increasing. There are still many difficulties surrounding research on the mechanism and development of therapeutic vaccines. It is urgent to explore the pathogenic mechanism of viruses to help prevent and treat COVID-19. In our study, we downloaded two datasets related to COVID-19 (GSE150819 and GSE147507). By analyzing the high-throughput expression matrix of uninfected human bronchial organoids and infected human bronchial organoids in the GSE150819, 456 differentially expressed genes (DEGs) were identified, which were mainly enriched in the cytokine–cytokine receptor interaction pathway and so on. We also constructed the protein–protein interaction (PPI) network of DEGs to identify the hub genes. Then we analyzed GSE147507, which contained lung adenocarcinoma cell lines (A549 and Calu3) and the primary bronchial epithelial cell line (NHBE), obtaining 799, 460, and 46 DEGs, respectively. The results showed that in human bronchial organoids, A549, Calu3, and NHBE samples infected with SARS-CoV-2, only one upregulated gene CSF3 was identified. Interestingly, CSF3 is one of the hub genes we previously screened in GSE150819, suggesting that CSF3 may be a potential drug target. Further, we screened potential drugs targeting CSF3 by MOE; the top 50 drugs were screened by flexible docking and rigid docking, with 37 intersections. Two antiviral drugs (Elbasvir and Ritonavir) were included; Elbasvir and Ritonavir formed van der Waals (VDW) interactions with surrounding residues to bind with CSF3, and Elbasvir and Ritonavir significantly inhibited CSF3 protein expression.
Current Status and Potential Therapeutic Strategies for Using Non-coding RNA to Treat Diabetic Cardiomyopathy
Authors: Rai Amit K. , Lee Brooke , Gomez Ramesh , Rajendran Deepu , Khan Mahmood , Garikipati Venkata Naga Srikanth
Published: January 2021

Diabetic cardiomyopathy (DMCM) is the leading cause of mortality and morbidity among diabetic patients. DMCM is characterized by an increase in oxidative stress with systemic inflammation that leads to cardiac fibrosis, ultimately causing diastolic and systolic dysfunction. Even though DMCM pathophysiology is well studied, the approach to limit this condition is not met with success. This highlights the need for more knowledge of underlying mechanisms and innovative therapies. In this regard, emerging evidence suggests a potential role of non-coding RNAs (ncRNAs), including micro-RNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) as novel diagnostics, mechanisms, and therapeutics in the context of DMCM. However, our understanding of ncRNAs’ role in diabetic heart disease is still in its infancy. This review provides a comprehensive update on pre-clinical and clinical studies that might develop therapeutic strategies to limit/prevent DMCM.
Investigation of the Nature of CgCDPK and CgbHLH001 Interaction and the Function of bHLH Transcription Factor in Stress Tolerance in Chenopodium glaucum
Authors: Zhou Zixin , Wang Juan , Zhang Shiyue , Yu Qinghui , Lan Haiyan
Published: January 2021

Calcium-dependent protein kinase (CDPK) and its substrates play important roles in plant response to stress. So far, the documentation on the characterization of the CDPK and downstream interaction components (especially transcription factors, TFs) is limited. In the present study, an interaction between CgCDPK (protein kinase) (accession no. MW26306) and CgbHLH001 (TF) (accession no. MT797813) from a halophyte Chenopodium glaucum was further dissected. Firstly, we revealed that the probable nature between the CgCDPK and CgbHLH001 interaction was the phosphorylation, and the N-terminus of CgbHLH001, especially the 96th serine (the potential phosphorylation site) within it, was essential for the interaction, whereas the mutation of 96Ser to alanine did not change its nuclear localization, which was determined by the N-terminus and bHLH domain together. Furthermore, we verified the function of CgbHLH001 gene in response to stress by ectopic overexpression in tobacco; the transgenic lines presented enhanced stress tolerance probably by improving physiological performance and stress-related gene expression. In conclusion, we characterized the biological significance of the interaction between CDPK and bHLH in C. glaucum and verified the positive function of CgbHLH001 in stress tolerance, which may supply more evidence in better understanding of the CDPK signaling pathway in response to adversity.
Leaf Multi-Element Network Reveals the Change of Species Dominance Under Nitrogen Deposition
Authors: Zhang Jiahui , Ren Tingting , Yang Junjie , Xu Li , Li Mingxu , Zhang Yunhai , Han Xingguo , He Nianpeng
Published: January 2021

Elements are important functional traits reflecting plant response to climate change. Multiple elements work jointly in plant physiology. Although a large number of studies have focused on the variation and allocation of multiple elements in plants, it remains unclear how these elements co-vary to adapt to environmental change. We proposed a novel concept of the multi-element network including the mutual effects between element concentrations to more effectively explore the alterations in response to long-term nitrogen (N) deposition. Leaf multi-element networks were constructed with 18 elements (i.e., six macronutrients, six micronutrients, and six trace elements) in this study. Multi-element networks were species-specific, being effectively discriminated irrespective of N deposition level. Different sensitive elements and interactions to N addition were found in different species, mainly concentrating on N, Ca, Mg, Mn, Li, Sr, Ba, and their related stoichiometry. Interestingly, high plasticity of multi-element network increased or maintained relative aboveground biomass (species dominance) in community under simulated N deposition, which developed the multi-element network hypothesis. In summary, multi-element networks provide a novel approach for exploring the adaptation strategies of plants and to better predict the change of species dominance under altering nutrient availability or environmental stress associated with future global climate change.
Give Goods or Give Money? The Influence of Cause-Related Marketing Approach on Consumers’ Purchase Intention
Authors: Ye Shenghong , Liu Yunxiao , Gu Suizi , Chen Haiquan
Published: January 2021

With the progress of social and improvement of public awareness, the demand for enterprises to participate in a social welfare cause is increasing. A company can directly support corporate social responsibility issues through cause-related marketing (CRM) approaches, for example, by donating part of the proceeds from product sales (i.e., buy-one give-money or BOGM) or simply by donating their products (i.e., buy-one give-one or BOGO). Previous research has only discussed the impact of one of these CRM approaches on customers in one study. This research compared the effect of these two approaches on the purchase intention of consumers. Experiment 1 demonstrated that, for practical products, the purchase intention of BOGO (vs. BOGM) was higher, while for hedonic products, the purchase intention of BOGM (vs. BOGO) was higher. More importantly, we found a potential mechanism – perceived helpfulness – that drives our main effect. Experiment 2 revealed that the different statement order of charity information and product information in advertising can moderate the main effect. The research also provides several implications and insight into how companies can make donations while winning more customers’ willingness to pay, thus encouraging more companies to fulfill their corporate social responsibility.
Introducing a Method for Intervals Correction on Multiple Likert Scales: A Case Study on an Urban Soundscape Data Collection Instrument
Authors: Lionello Matteo , Aletta Francesco , Mitchell Andrew , Kang Jian
Published: January 2021

Likert scales are useful for collecting data on attitudes and perceptions from large samples of people. In particular, they have become a well-established tool in soundscape studies for conducting in situ surveys to determine how people experience urban public spaces. However, it is still unclear whether the metrics of the scales are consistently interpreted during a typical assessment task. The current work aims at identifying some general trends in the interpretation of Likert scale metrics and introducing a procedure for the derivation of metric corrections by analyzing a case study dataset of 984 soundscape assessments across 11 urban locations in London. According to ISO/TS 12913-2:2018, soundscapes can be assessed through the scaling of 8 dimensions: pleasant, annoying, vibrant, monotonous, eventful, uneventful, calm, and chaotic. The hypothesis underlying this study is that a link exists between correlations across the percentage of assessments falling in each Likert scale category and a dilation/compression factor affecting the interpretation of the scales metric. The outcome of this metric correction value derivation is introduced for soundscape, and a new projection of the London soundscapes according to the corrected circumplex space is compared with the initial ISO circumplex space. The overall results show a general non-equidistant interpretation of the scales, particularly on the vibrant-monotonous direction. The implications of this correction have been demonstrated through a Linear Ridge Classifier task for predicting the London soundscape responses using objective acoustic parameters, which shows significant improvement when applied to the corrected data. The results suggest that the corrected values account for the non-equidistant interpretation of the Likert metrics, thereby allowing mathematical operations to be viable when applied to the data.
A Meaning-Aware Cultural Tourism Intelligent Navigation System Based on Anticipatory Calculation
Authors: Meng Lei , Liu Yuan
Published: January 2021

To improve the personalized service of cultural tourism, anticipatory calculation has become an essential technology in the content design of intelligence navigation system. Culture tourism, as a form of leisure activity, is being favored by an increasing number of people, which calls for further improvements in the cultural consumption experience. An important component of cultural tourism is for tourists to experience intangible cultural heritage projects with local characteristics. However, from the perspective of user needs and the content adaptive system, there are few suitable intelligent navigation and user demand anticipatory systems for intangible cultural heritage content. Purple clay culture is one of the first batches of national intangible cultural heritage protection projects in China. Therefore, taking purple clay culture exhibition as an example, this paper attempts to analyze the personalized information demand of tourism consumption experience in intangible cultural heritage communication activities with affective computing and meaning-driven innovative design method, by taking the content design in the navigation system as the research object. This paper uses the theory of planned behavior to calculate the relationship between tourists’ attitude, experience behavior, and display information demand. The findings indicate two issues. First, tourists’ demand for the entertainment and leisure attributes of intangible cultural heritage is greater than the demand for educational function attributes. Second, the meaning elements of information can change tourists’ beliefs in intangible cultural heritage and affect their attitude and behavior toward such heritage. According to the research results, strengthening the meaning elements of specific group information can improve people’s cultural identity and tourism satisfaction. The research results provide the basis for the content design direction of future museum intelligent navigation systems.
Working While Ill Is Not Always Bad—Positive Effects of Presenteeism
Authors: Lohaus Daniela , Habermann Wolfgang , El Kertoubi Isam , Röser Florian
Published: January 2021

Presenteeism—going to work while ill—is a widespread phenomenon worldwide. Previous research has concentrated mainly on its negative effects. This study investigates the positive consequences of presenteeism derived from a comprehensive content model of presenteeism that was developed on the basis of negative effects. In a quantitative online-survey employees (N = 181) rated the degree of experienced or potential positive effects depending on whether they had worked while ill (75%) or not (25%) during the previous year. Results revealed that all postulated positive effects described in the content model were relevant. Most positive effects were rated significantly higher by participants who had shown presenteeism in comparison to those who had not. The positive effects significantly predicted presenteeism propensity (adjusted R2 = 0.20) for participants having shown presenteeism. In addition, an overall rating of positive effects was significantly related to presenteeism, however, to a lesser degree. Overall, the results demonstrate the applicability of the content model to positive effects of presenteeism. They point to the need for further investigation of them and their consideration for the management of presenteeism.
The Relationship of Self-Presentation, Psychological Needs, and Exercise Dependence in College Students With Overweight
Authors: Liu YiWen , Liu Hao , Liu ZhongQiang
Published: January 2021

Objectives

The objectives of the study were to explore the effect of self-presentation and psychological needs on exercise dependence and to provide an essential reference for preventing and inhibiting the production of exercise dependence in overweight college students.

Methods

The freshmen in two comprehensive universities accepted physical fitness tests, filled out the scales of self-presentation, psychological needs, and exercise dependence after obtaining their consent. A sample of 747 overweight college students who have regular exercise was screened using the formula of Fox sports participation and the standard of overweight for Chinese adults. Multiple regression, exploratory factor, and confirmatory factor analyses were used to analyze the related data.

Results

(1) In overweight college students, compared with the lower peers, those with higher physical attractiveness were more likely to suffer from detoxification of emotion, physiology, and vitality, and compared with the lower peers, those with higher self-presentation were more likely to appear in physiological abstinence. (2) The self-presentation of overweight male and female students has a significant positive influence on psychological needs (beta = 0.31, p < 0.01, and 0.37, p < 0.01, respectively, for males and females) and exercise dependence (beta = −0.21, p < 0.01, and 0.26, p < 0.01, respectively, for males and females). In contrast, psychological needs have a significant negative influence on exercise dependence (beta = −0.21, p < 0.01, and −0.26, p < 0.01, respectively, for males and females). (3) The psychological needs of overweight male and female college students were established as the mediating effect of self-presentation and exercise dependence. The mediating effect of psychological needs of females was higher than that of males (18.5 vs. 15.5%). The “ability display” of male and female students can affect “emotional distress” by “autonomy” and “competence.” The sense of relationship partially mediated the “attractiveness” of male and female students. Also, females on the one hand, rely on “weight control” by “competence” to produce some mediating effects on “physiological distress.” At the same time, the “ability display” by “competence” has a full mediating effect on “physiological distress.”

Conclusion

In the self-presentation of the overweight college students, the higher scores in “attractiveness,” “weight control,” and “ability display,” the higher the psychological needs and exercise dependence; the higher the autonomy, competence, and relationship, the less the emotional, physiological, and dynamic distress.
Effect of Item Order on Certain Psychometric Properties: A Demonstration on a Cyberloafing Scale
Authors: Şahin Murat Doğan
Published: January 2021

Many studies have been conducted on the effect of item order in self-report questionnaires on mean scores. This research aims to study the effect of item order on measurement invariance in addition to mean scores. To this end, two groups randomly obtained from the same sample were presented a fixed order form in which all items belonging to the same dimension were adjacent to each other, and a random order form in which the items were randomly sequenced respectively. The results obtained revealed a statistically significant difference between the mean scores of the two forms. In the next stage of the study, the fit indices obtained from the confirmatory factor analysis (CFA) applied to the two separate forms and the modification indices (MI) suggested by the software were compared. Both forms returned high modification suggestions for adjacent items or items presented near each other. Additionally, it was found that high χ2 reductions suggested by the MIs in one form resulted in low χ2 reductions in the other. Lastly, multiple group CFA (mg-CFA) was conducted to determine whether or not measurement invariance was achieved through different item order presentations of the scale. The findings indicate that measurement invariance could not be achieved even at the first stage of analysis. It may specifically be stated that presenting respondents items under the same dimension together ensures empirical findings congruent with theoretical structure.
A Virtual Reality Game to Assess OCD Symptoms
Authors: van Bennekom Martine J. , de Koning Pelle P. , Gevonden Martin J. , Kasanmoentalib M. Soemiati , Denys Damiaan
Published: January 2021

Background: Symptoms of obsessive-compulsive disorder (OCD) are often underreported by patients and mainly triggered in the patients private domain, making it harder for clinicians to recognize OCD. Virtual reality (VR) can be used to assess OCD symptoms in the clinician's office. We developed a VR game in order to provoke subjective and physiological OCD symptoms. We hypothesize that (1) the VR game provokes more OCD symptoms in patients compared to healthy controls, (2) performing virtual compulsions leads to a reduction in emotional responses in OCD patients and that (3) the severity of VR game provoked symptoms correlates with severity of OCD symptoms.

Methods: Participants played the VR game on a laptop while physiological measures were recorded simultaneously. We measured emotional responses, virtual compulsions and physiological arousal in response to our VR game in 26 OCD patients and 26 healthy controls. We determined correlations between emotional responses, virtual compulsions and OCD severity.

Results: We found higher levels of VR-provoked anxiety (U = 179.5, p = 0.004) and virtual compulsions in OCD patients compared to healthy controls (p = 0.001). There was a significant reduction in emotional responses after performing virtual compulsions in the OCD patients. The emotional responses and virtual compulsions did not correlate significantly with Y-BOCS scores. A baseline difference between patients and healthy controls was found in heart rate variability (HRV), but no significant change in HRV, heartrate and skin conductance was found during the VR game

Conclusions: Our study clearly shows our OCD VR game is capable of provoking more anxiety and virtual compulsions in patients with OCD compared to healthy controls. Providing a direct patient-rated measurement in the clinicians room, the VR game could help in assessing core OCD symptoms and recognizing OCD.

Clinical Trial Registry Number: Netherlands Trial Register NTR5935.
The Resilience of Social Service Providers and Families of Children With Autism or Development Delays During the COVID-19 Pandemic—A Community Case Study in Hong Kong
Authors: Wong Paul Waiching , Lam Yanyin , Lau Janet Siuping , Fok Hungkit
Published: January 2021

Background:Hong Kong is one of the earliest cities to have hampered by the COVID-19. When preventive public health measures are enforced, specific groups, who have already been facing inequality before the outbreak, are likely to become more overlooked and vulnerable.

Aim:This community case study aims to describe the additional needs of families of children with autism spectrum disorder and other developmental issues, as well as unexpected difficulties and challenges social service professionals encountered when delivering service and their solutions toward these challenges.

Methods:A focus group with 10 professionals providing the Caregiver Skills Training Program was conducted.

Results:Poor families of vulnerable children were found to be challenged, more than average, in finding daily necessities during the initial stage of the outbreak. Most vulnerable children displayed additional problematic behaviors and emotional problems during the quarantine. The social service professionals addressed the family needs by providing tangible resources and offering online training, workshops, and programs to meet their needs. Several important lessons were learned. First, technology know-how on conducting online training, workshop, and program could be a challenge to some social service professionals and the parents. Second, the professionals reported that they made huge efforts to produce guidelines in protecting services users' privacy, to equip themselves with necessary skills in executing privacy-protection measures, and to keep exploring for safer alternatives. Third, providing tele-services in online mode represented a different interaction pattern between social service professionals and service users, especially in the recruitment processes and group dynamics.

Conclusion:In comparison with other cities, Hong Kong has responded to the COVID-19 efficiently and effectively based on the citizen's strict adherence to behavioral advice and the innovative altruistic efforts from the multi-sectors in the community.
Quality of Life and Its Correlates in Alcohol Use Disorder Patients With and Without Depression in China
Authors: Huang Hui , Shen Hongxian , Ning Kui , Zhang Ruiling , Sun Wei , Li Bing , Jiang Haifeng , Wang Wenzheng , Du Jiang , Zhao Min , Yi Zhihua , Li Jing , Zhu Rongxin , Lu Shuiping , Xie Shiping , Wang Xiaoping , Fu Wei , Gao Chengge , Hao Wei
Published: January 2021

Objective: Alcohol use disorder (AUD) is a serious issue worldwide and frequently co-occurs with depression. However, the quality of life (QOL) of AUD patients with and without depression is not well studied in the Chinese Han population. The aim of this study was to investigate QOL and its correlates in AUD patients with and without depression in China.

Methods: Five hundred and fifteen psychiatric patients diagnosed with AUD were recruited. All these patients completed the Beck Depression Inventory (BDI) to assess depression, the Medical Outcome Study 36-Item Short Form Health Survey (SF-36) to evaluate QOL and the Alcohol Use Disorders Identification Test (AUDIT) to measure the severity of drinking.

Results: Compared with AUD patients without depression, those with depression had a lower QOL in all eight domains of the SF-36 (all P < 0.001), but were more willing to have alcohol-related treatment (P < 0.05). Negative correlations were noted between (i) the BDI total score and all eight domains of the SF-36 (all P < 0.001); and (ii) between the AUDIT total score and six domains of the SF-36 (all P < 0.05).

Conclusions: Depression impairs QOL in patients with AUD in China. Early intervention in comorbid depression to improve QOL is needed.
Where Do Early Career Researchers Stand on Open Science Practices? A Survey Within the Max Planck Society
Authors: Toribio-Flórez Daniel , Anneser Lukas , deOliveira-Lopes Felipe Nathan , Pallandt Martijn , Tunn Isabell , Windel Hendrik ,   on behalf of Max Planck PhDnet Open Science Group
Published: January 2021

Open science (OS) is of paramount importance for the improvement of science worldwide and across research fields. Recent years have witnessed a transition toward open and transparent scientific practices, but there is still a long way to go. Early career researchers (ECRs) are of crucial relevance in the process of steering toward the standardization of OS practices, as they will become the future decision makers of the institutional change that necessarily accompanies this transition. Thus, it is imperative to gain insight into where ECRs stand on OS practices. Under this premise, the Open Science group of the Max Planck PhDnet designed and conducted an online survey to assess the stance toward OS practices of doctoral candidates from the Max Planck Society. As one of the leading scientific institutions for basic research worldwide, the Max Planck Society provides a considerable population of researchers from multiple scientific fields, englobed into three sections: biomedical sciences, chemistry, physics and technology, and human and social sciences. From an approximate total population of 5,100 doctoral candidates affiliated with the Max Planck Society, the survey collected responses from 568 doctoral candidates. The survey assessed self-reported knowledge, attitudes, and implementation of different OS practices, namely, open access publications, open data, preregistrations, registered reports, and replication studies. ECRs seemed to hold a generally positive view toward these different practices and to be interested in learning more about them. Furthermore, we found that ECRs’ knowledge and positive attitudes predicted the extent to which they implemented these OS practices, although levels of implementation were rather low in the past. We observed differences and similarities between scientific sections. We discuss these differences in terms of need and feasibility to apply these OS practices in specific scientific fields, but additionally in relation to the incentive systems that shape scientific communities. Lastly, we discuss the implications that these results can have for the training and career advancement of ECRs, and ultimately, for the consolidation of OS practices.
Unfavorable Prognostic Effects of the Stem Cell Pluripotency Factor Sox2 in Feline Invasive Mammary Carcinomas
Authors: Truchot Yohan , Dagher Elie , Abadie Jérôme , Nguyen Frédérique
Published: January 2021

Background: Sex-determining Region Y (SRY)-box transcription factor-2 (Sox2) belongs to the “Yamanaka's factors,” necessary and sufficient to convert somatic cells into pluripotent stem cells. In breast cancers, Sox2 expression has been associated with poor prognosis, and resistance to therapy. The aims of this study were to determine the frequency of Sox2 positivity in feline invasive mammary carcinomas (FMCs), its relationships with other clinical-pathologic variables, and with patient outcomes.

Materials and Methods: This study relies on a previously described retrospective cohort of 180 FMCs, diagnosed in female cats treated by mastectomy alone, with 2-year follow-up. Sox2 (clone SP76), Estrogen Receptor alpha (ER), Progesterone Receptor (PR), Ki-67, Human Epidermal growth factor Receptor 2 (HER2), Androgen Receptor (AR), Bcl-2, Forkhead box protein A1 (FOXA1), basal markers and FoxP3-positive regulatory T cells (Tregs) were detected by automated immunohistochemistry. Sox2 expression was quantitated as an index (percentage of neoplastic cells demonstrating a positive nuclear signal). The FMCs were considered Sox2-positive at threshold >42%.

Results: Sox2 was not expressed in the normal mammary gland or in mammary hyperplasia without atypia, but was occasionally detected in atypical hyperplasia. In FMCs, the mean Sox2 index was 38 ± 30%, and 79/180 FMCs (44%) were Sox2-positive. Sox2 expression was associated with older age at diagnosis, lymphovascular invasion, high Ki-67 proliferation indexes, low PR and FOXA1 expression, and increased numbers of tumor-associated Tregs, but was not significantly associated with the clinical stage, histological types, and histological grade. By multivariate survival analysis, Sox2 was associated with poor cancer-specific survival (Hazard Ratio = 1.48, 95% confidence interval 1.04–2.11, p = 0.0292), independently of the pathologic tumor size, pathologic nodal stage, distant metastasis, and AR expression. A rare subgroup of FMCs characterized by an AR+Sox2–phenotype (19/180 cases, 11%) was associated with very favorable outcomes.

Conclusion: Sox2 expression was associated with poor cancer-specific survival of female cats with invasive mammary carcinomas, as previously reported in human breast cancer, but was more commonly expressed in cats than reported in breast cancers. Sox2 showed complementarity with AR in FMC prognostication.
Owner Personality, Owner-Dog Attachment, and Canine Demographics Influence Treatment Outcomes in Canine Behavioral Medicine Cases
Authors: Powell Lauren , Stefanovski Darko , Siracusa Carlo , Serpell James
Published: January 2021

Human and canine parameters can affect the development of canine behavior problems, although their influence on the dog's response to veterinary behavioral treatment remains unclear. This study aimed to investigate the possible associations between canine behavior following clinical intervention and canine demographic characteristics, owner personality and owner-dog attachment. The study included 131 dog-owner dyads who attended a veterinary behavioral service. Owners completed the C-BARQ at baseline, 3-months and 6-months, and the 10 Item Personality Inventory and Lexington Attachment to Pet Scale at baseline. Data were analyzed for the effect of clinical intervention on C-BARQ subscale scores using mixed effect models. Binary logistic regression models were used to analyze the association between behavior change and canine and owner parameters. Within 6-months of veterinary consultation, trainability increased (coefficient 0.03, p = 0.01) and chasing (coefficient −0.04, p = 0.02), separation-related behavior (coefficient −0.04, p = 0.01) and energy level (coefficient −0.04, p = 0.05) decreased. Treatment outcomes were associated with both canine and owner variables. Canine behavior at baseline was the most consistent predictor of behavior change with less desirable baseline behavior associated with greater odds of decreased problem behavior at three- and 6-months post-consultation across most C-BARQ subscales. Canine age and weight; owner conscientiousness, extraversion and openness; and owner-dog attachment were also associated with treatment outcomes for some behavioral categories. These findings could be used by veterinarians to formulate more accurate prognoses and provide owners with targeted advice to reduce the influence of background factors on the dog's response to clinical behavioral intervention.
An Assessment of Snail-Farm Systems Based on Land Use and Farm Components
Authors: Apostolou Konstantinos , Staikou Alexandra , Sotiraki Smaragda , Hatziioannou Marianthi
Published: January 2021

In this study, the structural and management characteristics of snail farms in Greece were analyzed to maximize sustainable food production. Objectives, such as the classification of farming systems and assessing the effects of various annual production parameters, were investigated. Data were collected (2017) via a questionnaire, and sampling was conducted in 29 snail farms dispersed in six different regions (Thrace, Central Macedonia, West Macedonia, Thessaly, Western Greece, and the Attica Islands). Descriptive statistics for continuous variables and frequencies for categorical variables were calculated. The similarity between farms was analyzed using nonmetric multidimensional scaling (nMDS). The average farm operation duration exceeded eight months and the mean annual production was 1597 kg of fresh, live snails. Results recorded five farming systems: elevated sections (7%), net-covered greenhouse (38%), a mixed system with a net-covered greenhouse (10%), open field (38%), and mixed system with an open field (7%). Snail farms differ in the type of substrate, available facilities, and equipment (60% similarity between most of the open field farms). The geographical location of a farms’ settlement affects productivity but also influences the duration of operation, especially in open field farms, due to their operation under a wide assortment of climatic types.
A Survey among Dog and Cat Owners on Pet Food Storage and Preservation in the Households
Authors: Morelli Giada , Stefanutti Davide , Ricci Rebecca
Published: January 2021

Background: Pet food storage plays a crucial role in maintaining the nutritional and sensory properties of purchased products over time. Methods: An online survey was developed to collect data regarding owners’ storage habits for both commercial and home-made diets. Results: The questionnaire was completed by 1545 dog owners and 676 cat owners. Pet and owner age played roles in the choice of the type of diet (commercial vs. home-cooked vs. raw meat-based) adopted. Kibble feeders (75.7%) usually bought one (50.1%) or two (24.6%) packages at a time, and most pets (64.4%) took a minimum four weeks to consume an entire bag. Almost half of the owners (43.5%) used a container to store pet food (plastic bins for 79.5%). Pet food was commonly stored in the kitchen (45.1%) and not exposed to direct light (94.5%); 23.6% of the kibble feeders said it might be exposed to high temperatures. Most commercial pet food feeders (67.3%) considered preservatives a potential health risk for pets. Among homemade diet feeders, 38.6% stored fish oil at room temperature. Conclusions: Pet owners should be educated in proper food storage management when receiving feeding instructions from veterinarians. More comprehensive information on the nature and importance of additives in pet food should be promoted by manufacturers.
Prevalence and Antimicrobial Resistance of Enteropathogenic Bacteria in Yellow-Legged Gulls (Larus michahellis) in Southern Italy
Authors: Russo Tamara Pasqualina , Pace Antonino , Varriale Lorena , Borrelli Luca , Gargiulo Antonio , Pompameo Marina , Fioretti Alessandro , Dipineto Ludovico
Published: January 2021

Wild birds may host and spread pathogens, integrating the epidemiology of infectious diseases. Particularly, Larus spp. have been described as responsible for the spread of many enteric diseases, primarily because of their large populations at landfill sites. The aim of this study was to examine the role of yellow-legged gulls as a source of enteropathogenic bacteria such as Campylobacter spp., Salmonella spp., Shiga toxin-producing Escherichia coli and Yersinia spp., with particular attention to antibiotic-resistant strains. Enteropathogenic bacteria were isolated from 93/225 yellow-legged gulls examined from April to July, during a four-year period (2016–2019). Specifically, Campylobacter spp. was isolated from 60/225 samples (26.7%), and identified as C. coli (36/60) and as C. jejuni (24/60). Salmonella spp. was isolated from 3/225 samples (1.3%), and identified as Salmonella arizonae. Shiga toxin-producing E. coli were isolated from 30/225 samples (13.3%) samples, and serotyped as E. coli O128 (12/30) O26 (9/30), O157 (6/30) and O11 (3/30); Yersinia spp. was never detected. Isolated strains exhibited multidrug resistance, including vitally important antibiotics for human medicine (i.e., fluoroquinolones, tetracyclines). Our study emphasizes the importance of yellow-legged gulls as potential reservoirs of pathogenic and resistant strains and their involvement in the dissemination of these bacteria across different environments, with resulting public health concerns.
Cape Feather Coloration Signals Different Genotypes of the Most Polymorphic MHC Locus in Male Golden Pheasants (Chrysolophus pictus)
Authors: Liu Hong-Yi , He Ke , Ge Yun-Fa , Wan Qiu-Hong , Fang Sheng-Guo
Published: January 2021

Ornamental feather coloration is usually a reflection of male quality and plays an important role during courtship, whereas the essence of male quality at the genetic level is not well understood. Major histocompatibility complex (MHC)-based mate choice has been observed in various vertebrates. Here, we investigated the relationship between the coloration of cape feathers and the MHC genotypes in golden pheasants (Chrysolophus pictus). We found that feather coloration differed sharply among different individuals (brightness: 1827.20 ± 759.43, chroma: 1241.90 ± 468.21, hue: 0.46 ± 0.06). Heterozygous individuals at the most polymorphic MHC locus (IA2) had brighter feathers than homozygous individuals (Z = −2.853, p = 0.004) and were more saturated in color (Z = −2.853, p = 0.004). However, feather coloration was not related to other MHC loci or to overall genetic heterozygosity (p > 0.050). Our study suggested that coloration of cape feathers might signal IA2 genotypes in golden pheasants.
The Medial-Lateral Pedal Force Component Correlates with Q-Angle during Steady-State Cycling at Different Workloads and Cadences
Authors: Fonda Borut , Babič Jan , Šarabon Nejc
Published: January 2021

Leg movement during cycling is constrained to the pedal/crank path and predominantly occurs in a sagittal plane. Medial-lateral force (FML) applied to the pedals is considered as a waste and does not contribute to the pedaling. The aim of this study was to examine the changes in FML across different cadences and workloads, and to examine the correlation with lateral knee movement (Q-angle). Twenty-two cyclists completed six trials at three workloads (2, 2.5 and 3 W/kg) and three cadences (75, 85, 95 rpm). Forces were recorded from the force pedal mounted to the left side. Absolute and normalized (to the peak total force) FML were compared across conditions and cross-correlation with Q-angle was calculated. Absolute FML was significantly different across cadences and workloads (p < 0.05) with higher absolute FML at higher cadence. There was no significant difference in normalized FML across the three cadences. There was a significant decrease in normalized FML (~10 N) at higher workloads. Statistically significant correlations were found between the FML and Q-angle (R = 0.70–0.77). The results demonstrate the link between the FML and Q-angle in healthy pain-free cyclists during stationary cycling. It has also been observed that smaller normalized magnitude of the FML is present when the force effectiveness is increased.
High-Resolution Simulation of Polar Lows over Norwegian and Barents Seas Using the COSMO-CLM and ICON Models for the 2019–2020 Cold Season
Authors: Revokatova Anastasia , Nikitin Michail , Rivin Gdaliy , Rozinkina Inna , Nikitin Andrei , Tatarinovich Ekaterina
Published: January 2021

The lack of meteorological observations at high latitudes and the small size and relatively short lifetime of polar lows (PLs) constitute a problem in the simulation and prediction of these phenomena by numerical models. On the other hand, PLs, which are rapidly developing, can lead to such extreme weather events as stormy waves, strong winds, the icing of ships, and snowfalls with low visibility, which can influence communication along the Arctic seas. This article is devoted to studying the possibility of the numerical simulation and prediction of polar lows by different model configurations and resolutions. The results of the numerical experiments for the Norwegian and Barents seas with grid spacings of 6.5 and 2 km using the ICON-Ru configurations of the ICON (ICOsahedral Nonhydrostatic) model and with a grid spacing of 6.5 km using the COSMO-CLM (Climate Limited-area Modeling) configuration of the COSMO (COnsortium for Small-scale MOdelling) model are presented for the cold season of 2019–2020. All the used model configurations demonstrated the possibility of the realistic simulation of polar lows. The ICON model showed slightly more accurate results for the analyzed cases. The best results showed runs with lead times of less than a day.