bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Ficus nitida Allelopathic Potential and the Most Efficient Application Method for Controlling Weeds Associated with Sunflower Plant
Authors: El–Wakeel Mona Adel , Ahmed Salah El-Din Abd El-Ghany Ahmed , Messiha Nadia Khalil
Published: February 2023

Allelopathy is a phenomenon by which plants positively or negatively affect neighboring plants by releasing allelopathic compounds. These allelochemicals are secondary metabolites found in different concentrations in shoots, roots, leaves, flowers, and even pollen grains. Allelochemicals have potential as natural bioherbicides for controlling weeds when applied in the form of extracts or through intercropping, cover cropping, and mulching. The present study was conducted to investigate the allelopathic potential of Ficus nitida leaves against Echinochloa crus-galli L. and Corchorus olitorius L weeds associated with sunflower plant via two application methods (mixing of F. nitida leaf powder with soil and foliar spray of F. nitida leaf powder alcoholic extract). Two pot experiments were carried out for the two summer seasons of 2020 and 2021 in the greenhouse of the National Research Centre (NRC), Dokki, Giza, Egypt. Nine treatments were applied in a completely randomized block design. Three treatments were applied before sowing, namely F. nitida leaf powder was mixed with the soil at rates of 15, 30, and 45 g/pot. The other three treatments of alcoholic leaf powder extract of F. nitida were sprayed twice on both plants and weeds at 10, 20, and 30% (w/v) concentrations. Additionally, three check treatments—healthy (sunflower only), unweeded, and both weeds only—were applied for comparison. The recorded results showed that F. nitida had an allelopathic bioherbicidal effect on both weeds. By increasing the concentration of F. nitida extract, the bioherbicidal potential increased. Moreover, the recorded results showed that foliar spray of alcoholic extract at 30% concentration was the superior application method for controlling weeds. Mixing of F. nitida leaf powder at 45 g/pot ranked second after this superior treatment. A noticeable result is that these two superior treatments improved sunflower growth parameters and yield traits. Quantitative estimation of phenolic compounds and flavonoids demonstrated that the concentration of these allelochemicals is higher in alcoholic extract than in water extract. Moreover, phenolic acid concentrations as detected by high-performance liquid chromatography fractionation are higher in alcoholic extract than in water extract.
Prioritizing rural populations in state comprehensive cancer control plans: a qualitative assessment
Authors: Hirschey Rachel , Rohweder Catherine , Zahnd Whitney E. , Eberth Jan M. , Adsul Prajakta , Guan Yue , Yeager Katherine A. , Haines Heidi , Farris Paige E. , Bea Jennifer W. , Dwyer Andrea , Madhivanan Purnima , Ranganathan Radhika , Seaman Aaron T. , Vu Thuy , Wickersham Karen , Vu Maihan , Teal Randall , Giannone Kara , Hilton Alison , Cole Allison , Islam Jessica Y. , Askelson Natoshia
Published: February 2023

Purpose

The Centers for Disease Control and Prevention’s National Comprehensive Cancer Control Program (NCCCP) requires that states develop comprehensive cancer control (CCC) plans and recommends that disparities related to rural residence are addressed in these plans. The objective of this study was to explore rural partner engagement and describe effective strategies for incorporating a rural focus in CCC plans.

Methods

States were selected for inclusion using stratified sampling based on state rurality and region. State cancer control leaders were interviewed about facilitators and barriers to engaging rural partners and strategies for prioritizing rural populations. Content analysis was conducted to identify themes across states.

Results

Interviews (n = 30) revealed themes in three domains related to rural inclusion in CCC plans. The first domain (barriers) included (1) designing CCC plans to be broad, (2) defining “rural populations,” and (3) geographic distance. The second domain (successful strategies) included (1) collaborating with rural healthcare systems, (2) recruiting rural constituents, (3) leveraging rural community–academic partnerships, and (4) working jointly with Native nations. The third domain (strategies for future plan development) included (1) building relationships with rural communities, (2) engaging rural constituents in planning, (3) developing a better understanding of rural needs, and (4) considering resources for addressing rural disparities.

Conclusion

Significant relationship building with rural communities, resource provision, and successful strategies used by others may improve inclusion of rural needs in state comprehensive cancer control plans and ultimately help plan developers directly address rural cancer health disparities.
Fine-scale functional connectivity of two syntopic pond-breeding amphibians with contrasting life-history traits: an integrative assessment of direct and indirect estimates of dispersal
Authors: Martínez-Gil Helena , Sánchez-Montes Gregorio , Montes-Gavilán Pedro , Ugarte Gorka , Martínez-Solano Íñigo
Published: February 2023

Assessing patterns of functional connectivity among amphibian demes is crucial to unravel their population dynamics and prevent their isolation and eventual extinction. Integrative studies based on direct (capture-mark-recapture) and indirect (genetic) estimates of dispersal provide robust, biologically realistic inferences on population structure and connectivity, with applications for conservation efforts. We focused on two pond-breeding amphibians with contrasting life-history traits: the short-lived, semi-arboreal Hyla molleri and the long-lived, fossorial Pelobates cultripes. We PIT-tagged 2150 individuals of both species in two ponds (Laguna and Gravera, separated by 700 m) and monitored them from 2009 to 2021 to document the frequency and spatial extent of dispersal events. In addition, we genotyped individuals from these and two additional breeding populations at a maximum distance of 5 km with 15–16 microsatellites to characterize fine-scale patterns of genetic structure. We detected dispersal events connecting Laguna and Gravera in both species, albeit at low frequencies (4.8% and 7.7% of recaptured individuals of H. molleri and P. cultripes, respectively). However, both species were capable of covering long distances, with individual accumulated displacements up to 3.5 km (Hyla) and 1.8 km (Pelobates). Breeding populations > 2 km apart were genetically differentiated, indicating lower connectivity at this spatial scale. Estimates of pairwise migration rates differed between species and were asymmetrical, with different ponds representing “source” populations contributing more migrants to other populations in each species. We discuss the role of differences in life history traits and ecological preferences in shaping population dynamics in the two species and highlight management implications of our results.
The Maltese single transferable vote experience: a case study of gerrymandering?
Authors: Hasancebi Serhat
Published: February 2023

Re-drawing the electoral boundaries to provide benefit to one particular political party and thereby damaging the principle of representation in democracy has been a core issue in political science in the recent years. For years social scientists have been advocating the idea of measuring or preventing the potential for damage that may arise from the existence of the above-mentioned redistricting process. Following this discussion, we investigate the possible gerrymandering phenomenon that might have arisen, or whether there are any asymmetries or partisan biases due to boundary delimitation of the electoral constituencies in the case of the Maltese general elections. From the evidence of various statistical tests and simulations, our conclusion is that we find no evidence of gerrymandering in the 2013 and 2017 Maltese general elections.
Effects of the Prevention Program “HateLess. Together against Hatred” on Adolescents’ Empathy, Self-efficacy, and Countering Hate Speech
Authors: Wachs Sebastian , Krause Norman , Wright Michelle F. , Gámez-Guadix Manuel
Published: February 2023

Currently, there is a lack of empirically evaluated prevention programs targeting hate speech among adolescents. This is problematic because hate speech jeopardizes adolescents’ well-being and social integration. To this end, this study aims to evaluate the short-term effects of the newly developed anti-hate speech prevention program, “HateLess. Together against Hatred”, on adolescents’ empathy, self-efficacy, and counter-speech. Eight hundred and twenty adolescents between 12 and 16 (M = 13.27,SD = 1.04) from 11 German schools participated in this study. More specifically, 567 adolescents participated in the one-week prevention program, and 253 participants were assigned to the control group. Repeated measures ANOVAs showed that HateLess was successful, as there was a significant increase in empathy, self-efficacy, and counter-speech in the intervention group from the pretest (T1) to the posttest (T2) one month after the intervention. In contrast, no changes were found among adolescents in the control group. A multilevel mediation model revealed that the effect of being a member of the intervention group on counter-speech was partially mediated via empathy and self-efficacy. The findings indicate that HateLess is an effective, cost-efficient approach to enhance adolescents’ counter-speech directly and indirectly by altering the skills they need to become informed citizens in democratic societies.
Effect of poly (acrylamide-co-acrylic acid salt) on anti-aging properties and adhesion between acrylonitrile butadiene rubber and polyester fabric
Authors: Abdel‐Hakim Ahmed , El‐Wakil Abd El‐Aziz Arafa , Halim Sawsan
Published: February 2023

In this study, a multifunction polymeric adhesion promoter and anti-aging compound based on the triethanolamine salt of acrylamide acrylic acid copolymer (COS) was prepared and characterized using Fourier-transform infrared spectroscopy (FTIR). The impact of different COS contents on the tensile, adhesion, and thermal properties of NBR composite and NBR/PET sandwich was evaluated. The NBR composites containing COS displayed good retention of their mechanical properties with increasing thermal ageing time, while the composite without COS showed a decrease in its mechanical properties. The highest tensile strength (17.5 MPa with a retention value of 0.6%) after 7 days of thermal aging was recorded for NBR composite, which contains 5 phr (parts per hundred parts of rubber) of COS (COS 5), compared to NBR composite without COS (COS 0), which recorded 15.1 MPa with a retention value of -27.4%. In addition, the COS 5 composite displayed an improvement in peel strength of 16.4% compared to the COS 0. The results of the thermogarvemetric analysis (TGA) supported the anti-thermal ageing effect of COS, where the initial decomposition temperature (Ti) value increased by 11.7 and 9.3 °C, after addition of 5 and 10 phr of COS to NBR composite, respectively. In addition, the other thermogravemetric parameters investigated displayed a significant increase in their values, which confirms the improvement in thermal stability of NBR composite in the presence of COS. Also, the air permeability of the PET/NBR sandwich decreased by 80% after the addition of 7.5 phr of COS.
The multiomic landscape of meningiomas: a review and update
Authors: Wang Justin Z. , Nassiri Farshad , Landry Alexander P. , Patil Vikas , Liu Jeff , Aldape Kenneth , Gao Andrew , Zadeh Gelareh
Published: February 2023

Purpose

Meningiomas are the most common primary brain tumor in adults. Traditionally they have been understudied compared to other central nervous system (CNS) tumors. However over the last decade, there has been renewed interest in uncovering the molecular topography of these tumors, with landmark studies identifying key driver alterations contributing to meningioma development and progression. Recent work from several independent research groups have integrated different genomic and epigenomic platforms to develop a molecular-based classification scheme for meningiomas that could supersede histopathological grading in terms of diagnostic accuracy, biological relevance, and outcome prediction, keeping pace with contemporary grading schemes for other CNS tumors including gliomas and medulloblastomas.

Methods

Here we summarize the studies that have uncovered key alterations in meningiomas which builds towards the discovery of consensus molecular groups in meningiomas by integrating these findings. These groups supersede WHO grade and other clinical factors in being able to accurately predict tumor biology and clinical outcomes following surgery.

Results

Despite differences in the nomenclature of recently uncovered molecular groups across different studies, the biological similarities between these groups enables us to likely reconciliate these groups into four consensus molecular groups: two benign groups largely dichotomized by NF2-status, and two clinically aggressive groups defined by their hypermetabolic transcriptome, and by their preponderance of proliferative, cell-cycling pathways respectively.

Conclusion

Future work, including by our group and others are underway to validate these molecular groups and harmonize the nomenclature for routine clinical use.
Persistence and Structure
Authors: Sattig Thomas
Published: February 2023

Perdurance is a mode of persistence. The heart of perdurance is a space-time analogy: a perduring object is extended in time in a way that is analogous to how a composite object is extended in space. This paper is a discussion of perdurance in light of the distinction between mereologically structured and unstructured objects. I show that while the standard formulation of perdurance captures the space-time analogy for unstructured objects, it fails to capture the space-time analogy for temporally and spatially structured objects. I conclude that there are substantially different ways for an object to be space-like temporally extended.
Factors influencing the perceived value of travel time in European urban areas
Authors: Pourhashem Ghadir , Georgouli Christina , Malichová Eva , Straka Milan , Kováčiková Tatiana
Published: February 2023

This research aims at expanding the scope of travel satisfaction by incorporating subjective elements in the evaluation of worthwhileness of travel time proposed by the H2020 MoTiV project, using a European-wide mobility dataset collected in 2019. Trip characteristics, mood, socio-demographic characteristics, experience factors, travel activities and weather were analysed to explore their influence on travellers’ perception of worthwhileness of travel time. The analysis was performed separately for five different transport mode categories using Structural Equation Models. The empirical analysis of this research indicates the high significance of enjoyment in terms of making a trip worthwhile for all transport modes. The results also revealed mode-specific experience factors that play an important role in shaping travel experience which could be addressed to improve the quality of travel time. It is expected that these results can support a shift towards a more human-centric approach in urban mobility plans as well as pave the way for more inclusive transport policies.
The impact of necrotizing soft tissue infections on the lives of survivors: a qualitative study
Authors: Suijker Jaco , Stoop Matthea , Meij-de Vries Annebeth , Pijpe Anouk , Boekelaar Anita , Egberts Marthe , Van Loey Nancy
Published: February 2023

Purpose

Necrotizing soft tissue infections (NSTI) are potentially lethal infections marked by local tissue destruction and systemic sepsis, which require aggressive treatment. Survivors often face a long recovery trajectory. This study was initiated to increase understanding of the long-term impact of NSTI on health related quality of life (HRQoL), and how care may be improved.

Methods

Thematic analysis was applied to qualitative data from 25 NSTI-survivors obtained through two focus groups (n = 14) and semi-structured interviews (n = 11).

Results

The median age of the participants was 49 years, 14 were female. The median time since diagnosis was 5 years. Initial misdiagnosis was common, causing delay to treatment. Survivors experienced long-term physical consequences (scarring, cognitive impairment, fatigue, sleeping problems, recurrent infections), psychological consequences (traumatic stress symptoms, fear of relapse, adjusting to an altered appearance, sexual issues) and social and relational consequences (changes in social contacts, a lack of understanding). The disease also had a major psychological impact on family members, as well as major financial impact in some. There was a strong desire to reflect on ‘mistakes’ in case of initial misdiagnosis. To improve care, patient and family centered care, smooth transitions after discharge, and the availability of understandable information were deemed important.

Conclusion

This study reveals that NSTI have a large impact on physical and psychosocial wellbeing of survivors and their relatives. Except for a few differences (misdiagnosis, fear for re-infection and actual re-infection), the patient experience of patients with NSTI is largely similar to those of burn survivors. Thus, questionnaires to assess HRQoL in burn survivors may be used in future NSTI studies.
Do learners need semantics to spell syntactic markers? Plural spellings in real vs. pseudowords in a French L2 setting
Authors: Klasen Lisa , Ugen Sonja , Dording Carole , Fayol Michel , Weth Constanze
Published: February 2023

Inaudible syntactic markers are especially difficult to spell. This paper examines how 455 fourth graders spell silent French plural markers in a dictation with real and pseudowords after one year of formal French instruction (L2). The Generalized Linear Mixed Model analysis shows first that noun plural spelling (real and pseudo) is a strong predictor for verb and adjective plural spelling. Second, the performance on real verb plural is higher than the performance on real adjective plural. In contrast, the performance on pseudoadjective plural is higher than on pseudoverb plural. Our findings indicate the strong influence of semantics and frequency in instruction input on plural spelling: noun plural is semantically grounded, and nouns are most frequent in the curriculum. Verbs and verb plural are also frequent, and inflection is mostly taught by means of memorizing the verb inflection paradigm. Adjectives are taught least frequently. The findings are discussed in the context of French L2 instruction, as the extremely low results on adjectives and pseudoverbs seem to be a consequence of instruction methods.
RETRACTED ARTICLE: Robust adversarial uncertainty quantification for deep learning fine-tuning
Authors: Ahmed Usman , Lin Jerry Chun-Wei
Published: February 2023

This paper proposes a deep learning model that is robust and capable of handling highly uncertain inputs. The model is divided into three phases: creating a dataset, creating a neural network based on the dataset, and retraining the neural network to handle unpredictable inputs. The model utilizes entropy values and a non-dominant sorting algorithm to identify the candidate with the highest entropy value from the dataset. This is followed by merging the training set with adversarial samples, where a mini-batch of the merged dataset is used to update the dense network parameters. This method can improve the performance of machine learning models, categorization of radiographic images, risk of misdiagnosis in medical imaging, and accuracy of medical diagnoses. To evaluate the efficacy of the proposed model, two datasets, MNIST and COVID, were used with pixel values and without transfer learning. The results showed an increase of accuracy from 0.85 to 0.88 for MNIST and from 0.83 to 0.85 for COVID, which suggests that the model successfully classified images from both datasets without using transfer learning techniques.
Emerging mercury and methylmercury contamination from new artisanal and small-scale gold mining along the Nile Valley, Egypt
Authors: Abdelaal Ahmed , Sultan Mohamed , Abotalib Abotalib Z. , Bedair Mohamed , Krishnamurthy R. V. , Elhebiry Mohamed
Published: February 2023

The past decade witnessed the initiation and boom of the Artisanal and Small-scale Gold Mining (ASGM) activities in the hyper-arid southern Egypt. The ores are mined in the Eastern Desert and then transported to the densely populated farming communities in the Nile Valley, where the river provides the water resources needed for ore processing. In search for economic benefits, the poorly educated farmers with limited technical resources transformed their cultivated lands into ASGM operations, exposing themselves, their families, the residents, and the Nile ecosystems to several environmental and occupational health problems. Using integrated remote sensing, field, geochemical, and isotopic analyses, we report the first inventory of ASGM-related total mercury (THg) and methylmercury (MeHg) levels in tailings, amalgamation-tailing ponds, and surface and groundwater with emphasis on the Edfu city and its surroundings. The field and remote sensing-based mapping of ASGM activities reveals clustering around the Nile waterways and suggests interaction of Hg contamination sources with their surrounding receptors. Common ASGM practices include release of contaminated water from unlined amalgamation-tailing ponds into irrigation and drainage canals, and spreading of tailings over cultivated soils. In a short period (10 years), the released Hg contaminated multiple media, including the surface water, the shallow and deep aquifers, and possibly the soil, crops, and livestock. THg levels in amalgamation-tailing ponds (1200–8470 ng/L) are fourfold higher than US EPA and eightfold the WHO thresholds. The contaminated waters released from amalgamation-tailing ponds raised THg levels in surface water (irrigation canals: 50–100 ng/L; drainage canals: THg: > 200 ng/L) and groundwater (shallow and deep aquifers: 80–500 ng/L). Our findings highlight the need to extend the adopted approach to cover the entire length of the Nile River and its valley and the importance of conducting awareness campaigns to educate residents and health care providers about potential ASGM-related environmental and health hazards.

Graphical Abstract
One Navier’s problem for the Brinkman system
Authors: Medková Dagmar
Published: February 2023

In this paper we study the Brinkman system and the Darcy-Forchheimer-Brinkman system with the boundary condition of the Navier’s type $$ {\textbf{u}}_{{\mathbf {\mathcal {T}}}} = {\textbf{g}}_{{\mathbf {\mathcal {T}}}} $$

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on $$\partial \Omega $$

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. Classical solutions are from the spaces $${\mathcal C}^{k+1,\gamma }(\overline{\Omega },{\mathbb R}^2) \times {\mathcal C}^{k,\gamma }(\overline{\Omega })$$

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. For the Brinkman system we show the unique solvability of the problem. Then we study the Navier problem for the Darcy-Forchheimer-Brinkman system and small boundary conditions.
A Circular RNA Expressed from the FAT3 Locus Regulates Neural Development
Authors: Seeler Sabine , Andersen Maria Schertz , Sztanka-Toth Tamas , Rybiczka-Tešulov Mateja , van den Munkhof Marleen H. , Chang Chi-Chih , Maimaitili Muyesier , Venø Morten Trillingsgaard , Hansen Thomas Birkballe , Pasterkamp R. Jeroen , Rybak-Wolf Agnieszka , Denham Mark , Rajewsky Nikolaus , Kristensen Lasse Sommer , Kjems Jørgen
Published: February 2023

Circular RNAs (circRNAs) are key regulators of cellular processes, are abundant in the nervous system, and have putative regulatory roles during neural differentiation. However, the knowledge about circRNA functions in brain development is limited. Here, using RNA-sequencing, we show that circRNA levels increased substantially over the course of differentiation of human embryonic stem cells into rostral and caudal neural progenitor cells (NPCs), including three of the most abundant circRNAs, ciRS-7, circRMST, and circFAT3. Knockdown of circFAT3 during early neural differentiation resulted in minor transcriptional alterations in bulk RNA analysis. However, single-cell transcriptomics of 30 and 90 days differentiated cerebral organoids deficient in circFAT3 showed a loss of telencephalic radial glial cells and mature cortical neurons, respectively. Furthermore, non-telencephalic NPCs in cerebral organoids showed changes in the expression of genes involved in neural differentiation and migration, including FAT4, ERBB4, UNC5C, and DCC. In vivo depletion of circFat3 in mouse prefrontal cortex using in utero electroporation led to alterations in the positioning of the electroporated cells within the neocortex. Overall, these findings suggest a conserved role for circFAT3 in neural development involving the formation of anterior cell types, neuronal differentiation, or migration.
Institutional quality and COVID-19 vaccination: does decentralization matter?
Authors: Del Bo Chiara F.
Published: February 2023

Vaccination campaigns are one of the factors that can help mitigate the adverse effects of viral pandemics. The aim of this paper is to understand the institutional factors that are associated with a higher success rate, measured by the percentage of vaccinated population against COVID-19 across countries. Along with supply side determinants, institutional factors, related, at the national level, to the organization of the healthcare sector, governance and organization of the State and social capital, and, at the subnational level related to the authority and autonomy of lower tiers of government, are important correlates of successful vaccination campaigns, suggesting potential areas of public policy interventions.
Stock control analytics: a data-driven approach to compute the fill rate considering undershoots
Authors: Babiloni Eugenia , Guijarro Ester , Trapero Juan R.
Published: February 2023

One of the most frequently used inventory policies is the order-point, order-up-to-level (s, S) system. In this system, the inventory is continuously reviewed and a replenishment request is placed whenever the inventory position drops to or below the order point, s. The variable replenishment order quantity and the variable replenishment cycle characterize the system by the use of complex mathematical computations. Different methodological approaches diminish the mathematical complexity by neglecting the undershoots, i.e., the quantity that the inventory position is below the order point when it is reached. In this paper, we conceptually and empirically analyse the bias that neglecting the undershoots introduces into the estimation of the fill rate. After that, we suggest a new methodology developed under a data-driven perspective that uses a state-dependent parameter algorithm to correct such a bias. As a result, we propose two new methods, one parametric and the other nonparametric, to enhance the fill rate estimate. Both methods, named analytics fill rate methods, remove the bias that neglecting the undershoots introduces and are used to illustrate the practical implications of this hypothesis on the performance and design of the (s, S) system. This research is developed in a lost sales context with simulated stochastic and i.i.d. discrete demands as well as actual sales data.
On the Role of Beliefs and Trust for the Intention to Use Service Robots: An Integrated Trustworthiness Beliefs Model for Robot Acceptance
Authors: Kraus Johannes , Miller Linda , Klumpp Marielène , Babel Franziska , Scholz David , Merger Julia , Baumann Martin
Published: February 2023

With the increasing abilities of robots, the prediction of user decisions needs to go beyond the usability perspective, for example, by integrating distinctive beliefs and trust. In an online study (N = 400), first, the relationship between general trust in service robots and trust in a specific robot was investigated, supporting the role of general trust as a starting point for trust formation. On this basis, it was explored—both for general acceptance of service robots and acceptance of a specific robot—if technology acceptance models can be meaningfully complemented by specific beliefs from the theory of planned behavior (TPB) and trust literature to enhance understanding of robot adoption. First, models integrating all belief groups were fitted, providing essential variance predictions at both levels (general and specific) and a mediation of beliefs via trust to the intention to use. The omission of the performance expectancy and reliability belief was compensated for by more distinctive beliefs. In the final model (TB-RAM), effort expectancy and competence predicted trust at the general level. For a specific robot, competence and social influence predicted trust. Moreover, the effect of social influence on trust was moderated by the robot's application area (public > private), supporting situation-specific belief relevance in robot adoption. Taken together, in line with the TPB, these findings support a mediation cascade from beliefs via trust to the intention to use. Furthermore, an incorporation of distinctive instead of broad beliefs is promising for increasing the explanatory and practical value of acceptance modeling.
Markedly divergent effects of Ouabain on a Temozolomide-resistant (T98G) vs. a Temozolomide-sensitive (LN229) Glioblastoma cell line
Authors: Weidemann Heidrun , Feger Daniel , Ehlert Jan E. , Menger Marcus M. , Krempien Robert C.
Published: February 2023

Background

Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor with poor prognosis. GMB are highly recurrent mainly because of radio- and chemoresistance. Radiotherapy with Temozolomide (TMZ) is until today the golden standard adjuvant therapy, however, the optimal treatment of recurrent glioblastoma remains controversial. Ouabain belongs to the Cardiotonic Steroids (CTS) the natural ligands of the Na/K-ATPase (NKA). It is established that the NKA represents a signal transducer with either stimulating or inhibiting cell growth, apoptosis, migration and angiogenesis. Over the last decade evidence grew that CTS have anti-tumor properties especially in GBM.

Aim

Proceeding from recent studies we wanted to further demonstrate a divergent effect of Ouabain on a TMZ-resistant (T98G) as compared to a TMZ-sensitive (LN229) GBM cell line.

Methods

We analyzed the effect of Ouabain on cell migration and plasma cell membrane potential (PCMP) in the LN229 and T98G GBM cell line as well as underlying mechanisms (Bcl-2 and p-Akt/pan-Akt expression). Moreover, we analyzed the anti-angiogenic effect of Ouabain on human umbilical vein endothelial cells (HUVECs).

Results

T98G cells showed a significant inhibition of cell migration and a significant depolarization of the PCMP at similar Ouabain concentrations (IC50 = 1.67 × 10–7 M) resp. (IC50 = 2.72 × 10–7 M) with a strong inverse correlation (R2 = 0.95). In contrast, LN229 cells did not respond to Ouabain in these assays at all. Similarly, only T98G but not LN229 cells revealed Bcl-2 down-regulation at nanomolar Ouabain concentrations. This unique response to Ouabain is associated with a down-regulation of pan-Akt in T98G cells 24 h after Ouabain (1.0 × 10–6 M) treatment. For the first time, the anti-angiogenic effect of Ouabain on HUVEC cells (IC50 = 5.49 × 10–8 M) was demonstrated which correlated strongly with the anti-migratory effect (R2 = 0.85).

Conclusion

The TMZ-resistant T98G cell line as compared to the TMZ-sensitive LN229 cell line shows a high sensitivity towards Ouabain. We consider it as a promising new compound especially in recurrent GBM to overcome the resistance to TMZ and irradiation.
Influence of River Disconnection on Floodplain Periphyton Assemblages
Authors: De Gallardo Kamela , Kaller Michael D. , Rutherford D. Allen , Kelso William E.
Published: February 2023

The Atchafalaya River Basin (ARB) in southcentral Louisiana, USA, is a structurally and biotically diverse floodplain of Atchafalaya River (AR), which is the largest distributary of the Mississippi River. Annual floodplain inundation facilitates the exchange of nutrients and organic material between the AR and its floodplain, giving rise to the high productivity of the river-floodplain system. Production within the ARB is driven by periphytic algae, phytoplankton, and aquatic macrophytes, however, very little is known about periphytic algal assemblages in floodplain systems or how loss of annual flooding impacts these assemblages. In this study, we use artificial substrates to sample periphytic algae bi-weekly (January 2019 – September 2019) from ARB sites with active river connections and from a permanently-isolated floodplain system (Lake Verret). Our results showed that connection to the river caused spatiotemporal shifts in periphytic algal assemblages in the ARB. Overall, ARB sites had a higher density of algal cells compared with non-ARB sites, and for ARB sites with more active river connections, total algal density was greater nearer to river inputs, particularly for cyanobacteria and centric diatoms, with diatoms dominating periphyton assemblages year-round. In contrast, the river-isolated system was dominated largely by chlorophytes. In both isolated and connected systems, sites with heavy macrophyte cover showed increased densities of euglenoids, chrysophytes, and xanthophytes. Shifts in periphytic algal assemblages due to floodplain alterations, such as the disconnection of a floodplain from its river source, could impact higher trophic levels and should be considered in future wetland management decisions.
Utilizing historical maps in identification of long-term land use and land cover changes
Authors: Mäyrä Janne , Kivinen Sonja , Keski-Saari Sarita , Poikolainen Laura , Kumpula Timo
Published: February 2023

Knowledge in the magnitude and historical trends in land use and land cover (LULC) is needed to understand the changing status of the key elements of the landscape and to better target management efforts. However, this information is not easily available before the start of satellite campaign missions. Scanned historical maps are a valuable but underused source of LULC information. As a case study, we used U-Net to automatically extract fields, mires, roads, watercourses, and water bodies from scanned historical maps, dated 1965, 1984 and 1985 for our 900 km$$^2$$

2

study area in Southern Finland. We then used these data, along with the topographic databases from 2005 and 2022, to quantify the LULC changes for the past 57 years. For example, the total area of fields decreased by around 27 km$$^2$$

2

, and the total length of watercourses increased by around 2250 km in our study area.
Thermal degradation behaviour and chemical kinetic characteristics of biomass pyrolysis using TG/DTG/DTA techniques
Authors: El-Sayed Saad A. , Khass Tarek M. , Mostafa Mohamed E.
Published: February 2023

The goal of the current study is to investigate the thermal degradation of palm fronds (PF), olive leaves (OL), and wheat straw (WS) through pyrolysis and calculate their kinetic data using TG-DTG and DTA approaches. The kinetic parameters were assessed using isoconversional techniques like the Ozawa-Flynn-Wall (OFW) and Kissinger–Akahira–Sunose (KAS) methods, as well as model-fitting techniques like the integral method, which employs various diffusion and reaction order models. Using kinetics data models, typical parameters for pyrolysis and thermodynamics were estimated. For PF, OL, and WS, the values of activation energy (E) from the integral method ranged between 8.82 and 167.13, 23.06 and 149.20, and 11.01 and 156.27, respectively, for diffusion models. On the other hand, the values of (E) ranged between 22.3 and 117.49, 51.69 and 92.88, and 23.48 and 125.97, respectively, for reaction-order models. The average activation energies (E) calculated by using PF, OL, and WS samples are 91.9, 69.1, and 65.2, respectively, for the OFW method and 87.5, 101.8, and 63.4, respectively, for the KAS method. The results demonstrated that the integral method provided values of (E) that were almost identical to those produced by the KAS and OFW methods. In the same range of (α), results showed that reaction order models yielded greater frequency factor values than diffusion models, demonstrating how simpler and quicker pyrolysis is. The values of ($${\Delta \mathrm{G}}_{av}$$

Δ
G

av

) demonstrated the acceptability of these materials for pyrolysis, and for the OFW and KAS techniques, the sequence of the degradation process was OL > WS > PF. The calculated ($${\Delta \mathrm{G}}_{av}$$

Δ
G

av

) showed that more heat energies are required for OL, PF, and WS to dissociate the reagent bonds, which agrees with the (E) values derived from the OFW model.

Graphical Abstract
Nitrogen transformation during fermentation in agricultural biogas plants
Authors: Wilińska-Lisowska A. , Roubinek O. , Chmielwski R. , Czerwionka K.
Published: February 2023

The aim of the present study was to determine changes in the organic nitrogen fraction in the liquid fraction of the digestate during laboratory tests of mono- and co-digestion. Three agricultural wastes were tested: distillery residue, cattle slurry and corn silage. In the mono-fermentation tests, the initial total nitrogen concentration depended on the feedstock used. As a result of the hydrolysis and ammonification of the organic matter contained in the feedstocks, there was an increase in ammonium nitrogen concentration after 28 days of fermentation. Analysis of organic nitrogen fractions showed that different organic nitrogen fractions predominated in each of the feedstocks. Four co-digestion tests were performed with 20% and 80% inoculum content and the combination of feedstocks used in the mono-fermentation. Proper evaluation of the transformation of nitrogen forms for the tests with 20 and 80% inoculum participation required the calculation of concentrations from the mass balance, taking into account the effect of changes taking place in the inoculum itself (control test). For these tests, the initial concentrations of nitrogen forms are similar to those found in the mono-fermentation tests. A greater increase in ammonium nitrogen concentrations was found for the test with 80% inoculum than in the test with 20% (for the same feedstock). An increase in the removal efficiency of the dissolved organic nitrogen fraction (DON), and an increase for the colloidal (CON) and particulate organic nitrogen (PON) fractions after 28 days of co-digestion were observed. The trend of change was similar for 20% and 80% inoculum.
Human umbilical cord mesenchymal stem cell-derived TGFBI attenuates streptozotocin-induced type 1 diabetes mellitus by inhibiting T-cell proliferation
Authors: Wu Chushan , Liu Weijiang , Liu Yuanlin , Xu Tingting , Li Man , Li Xue , Wang Yang , Meng Guangyu , Li Lu , Zheng Rongxiu , Zhang Yi
Published: February 2023

MSCs have been demonstrated to have a great benefit for type 1 diabetes mellitus (T1DM) due to their strong immunosuppressive and regenerative capacity. However, the comprehensive mechanism is still unclear. Our previous study indicated that transforming growth factor beta induced (TGFBI) is highly expressed in human umbilical cord-derived mesenchymal stem or stromal cells (hUC-MSCs), which are also implicated in T1DM. In this study, we found that infusion of TGFBI knockdown hUC-MSCs displayed impaired therapeutic effects in T1DM mice and decreased immunosuppressive capability. TGFBI knockdown hUC-MSCs could increase the proportion of T-cell infiltration while increasing the expression of IFN-gamma and interleukin-17A in the spleen. In addition, we also revealed that hUC-MSC-derived TGFBI could repress activated T-cell proliferation by interfering with G1/S checkpoint CyclinD2 expression. Our results demonstrate that TGFBI plays a critical role in MSC immunologic regulation. TGFBI could be a new immunoregulatory molecule controlling MSC function for new treatments of T1DM.

Graphical Abstract

Schematic Representation of the Immunosuppression capacity of hUC-MSC by TGFBI
Short High-Intensity Interval Exercise for Workplace-Based Physical Activity Interventions: A Systematic Review on Feasibility and Effectiveness
Authors: Amatori Stefano , Ferri Marini Carlo , Gobbi Erica , Sisti Davide , Giombini Germana , Rombaldoni Rosalba , Rocchi Marco B. L. , Lucertini Francesco , Federici Ario , Perroni Fabrizio , Calcagnini Giorgio
Published: February 2023

Background

Workplace exercise interventions showed good results, but lack of time was often reported as a barrier to participation. To overcome this problem, several studies attempted to implement short high-intensity interval training (HIT) within the workplace.

Objectives

The aim of this systematic review is to explore the feasibility and effectiveness of HIT interventions within the workplace setting.

Data sources

A systematic literature search was conducted in PubMed and SPORTDiscus to identify articles related to HIT within the workplace.

Study eligibility criteria

Only interventions that consisted of HIT programmes within the workplace and tested at least one physiological, psychological, or work-related outcome were included.

Results

Seven studies (317 participants) met the inclusion criteria. HIT interventions lasted 6–12 weeks, with a frequency of 2–4 sessions/week and a duration of 8–30 min per session. Feasibility was qualitatively investigated in four studies, with key positive aspects reported for HIT time-appeal, the sense of competence driven by individual intensity, and improved intention to exercise; five studies reported adherence rates > 80%. Small-to-large effect sizes were reported for improvements in cardiorespiratory and muscular fitness. Small-to-medium effect sizes were reported for blood parameters and health-related quality of life.

Conclusions

HIT interventions in the workplace showed limited effectiveness in improving health-related outcomes, while promising results regarding feasibility were reported, mainly due to the time-efficiency and the positive post-exercise psychosocial responses. However, further high-quality studies involving more participants are still needed to make firm conclusions on HIT effectiveness and feasibility compared to other types of exercise in this context.
An Overview of Stochastic Quasi-Newton Methods for Large-Scale Machine Learning
Authors: Guo Tian-De , Liu Yan , Han Cong-Ying
Published: February 2023

Numerous intriguing optimization problems arise as a result of the advancement of machine learning. The stochastic first-order method is the predominant choice for those problems due to its high efficiency. However, the negative effects of noisy gradient estimates and high nonlinearity of the loss function result in a slow convergence rate. Second-order algorithms have their typical advantages in dealing with highly nonlinear and ill-conditioning problems. This paper provides a review on recent developments in stochastic variants of quasi-Newton methods, which construct the Hessian approximations using only gradient information. We concentrate on BFGS-based methods in stochastic settings and highlight the algorithmic improvements that enable the algorithm to work in various scenarios. Future research on stochastic quasi-Newton methods should focus on enhancing its applicability, lowering the computational and storage costs, and improving the convergence rate.
Asthma Exacerbations in Severe Asthma: Why Systemic Corticosteroids May not Always Be the Best Treatment Option
Authors: McDowell P. Jane , Busby J. , Heaney Liam G.
Published: February 2023

Purpose

Advances in the management of severe, eosinophilic asthma have improved, but asthma exacerbations continue to occur. This review aims to look at the evidence we have about why exacerbations may occur; their phenotype and why oral corticosteroids may not always be the best treatment option for all exacerbation of symptoms in individuals with severe asthma.

Recent findings

Studies dating back to the 1990s showed that asthma exacerbations across the spectrum of asthma severity were of different inflammatory endotypes. In addition, there is a wealth of evidence suggesting that eosinophilic inflammation is very responsive to corticosteroid therapy, but that non-eosinophilic inflammation is less so. Two recent UK-based studies have undertaken systematic phenotyping of exacerbations in severe asthma and have shown that there are a significant minority of exacerbation events with an increase in asthma symptoms, fall in lung function, but without evidence of raised T2 biomarkers.

The evidence to date would suggest that T2 biomarker low asthma exacerbations do not benefit from the administration of oral corticosteroids; in fact, the effect of the oral corticosteroids is harmful. However, there is a paucity of data to answer this question directly. Further research is needed to assess the evolution of non-T2 exacerbations not treated with OCS in a randomised, placebo-controlled, manner.
In Graves’ disease, thyroid autoantibodies and ultrasound features correlate with distinctive histological features
Authors: Brancatella A. , Torregrossa L. , Viola N. , Sgrò D. , Casula M. , Basolo F. , Materazzi G. , Marinò M. , Marcocci C. , Santini F. , Latrofa F.
Published: February 2023

Purpose

Laboratory, imaging, and pathological features of Graves’ disease (GD), although well characterized, have been barely correlated each other. Aim of the study was to link laboratory and ultrasound characteristics of GD with its pathological features.

Methods

We correlated laboratory and ultrasound data at the time of diagnosis in 28 consecutive GD patients who underwent thyroidectomy with their pathological features, i.e., lymphocytic infiltration and follicular hyperplasia (both classified as mild or severe).

Results

Thyroid volume correlated positively with the levels of FT4 (P = 0.002, r2 = 0.42), FT3 (P = 0.011, r2 = 0.22), autoantibodies to thyroglobulin (TgAbs) (P = 0.016, r2 = 0.32), autoantibodies to thyroid peroxidase (TPOAbs) (P = 0.011, r2 = 0.34) and the extent of lymphocytic infiltration (P = 0.006 comparing mild to severe lymphocytic infiltration) but not with the levels of autoantibodies to the thyrotropin receptor (TRAbs) and to follicular hyperplasia. Compared to subjects with mild lymphocytic infiltration, those with severe lymphocytic infiltration showed higher levels of TgAbs (316 vs 0.0 IU/mL, P < 0.0001) and TPOAbs (295 IU/mL vs 14 IU/mL, P < 0.0001) and similar levels of TRAbs (7.5 vs 13 IU/mL, P = 0.68). Compared to patients with mild, those with severe follicular hyperplasia had similar levels of TgAbs (76 vs 30 IU/mL, P = 0.31) and TPOAbs (251 IU/mL vs 45 IU/mL, P = 0.26) but higher levels of TRAbs (39 vs 7.2 IU/mL, P < 0.001).

Conclusion

In GD, TgAbs and TPOAbs levels correlate with the extent of lymphocytic infiltration, TRAbs levels with the degree of follicular hyperplasia. Thyroid volume, the main factor influencing the severity of hyperthyroidism, is related to lymphocytic infiltration and not to follicular hyperplasia.