bluuebunny/crossref_metadata_2025 huggingface dataset:
The Giraffe as a Natural Animal Model for Resistance to Heart Failure with Preserved Ejection Fraction
Authors: Horowitz Barbara N. , Baccouche Basil , Shivkumar Tejas , Bertelsen Mads Frost , Aalkjær Christian , Smerup Morten , Ajijola Olujimi A. , Hadaya Joseph , Wang Tobias
Published: October 2020

Heart failure with preserved ejection fraction (HFpEF) is a leading form of human cardiovascular disease and commonly associated with systemic hypertension. Unique evolved adaptations in giraffe myocardia may be a natural animal model of resistance to HFpEF. In humans, pressure-overload induced left ventricular thickening (PLVT) impairs diastolic relaxation, elevates left atrial pressures and may progress to heart failure with symptoms including exercise intolerance. In healthy giraffe, the left ventricle thickens as developmental neck lengthening widens the vertical distance between the heart and head increasing pressures needed to maintain constant brain perfusion. Yet, diastolic relaxation and exercise capacity are unimpaired, a critical adaptation for prey species such as giraffe. The proximate mechanisms underlying this unique cardiovascular physiology are not yet characterized. Developmental PLVT in giraffe emerges as a species-specific evolved adaptation which offers a roadmap for identifying innovations in therapeutic and prevention strategies for HFpEF.
Knowledge and Attitude among Nyamagana Community Members towards Mental illness, Tanzania
Authors: Mvandal Samson Peter , Mponzi Kudra , Mushi Rosemary
Published: October 2020

Knowledge and attitude towards mental illness play major role in the recognition, management, sociocultural factors and health seeking behavior among those with mental disorders. The study aim was to determine the knowledge and attitude among Nyamagana community members towards mental illness, Tanzania; A descriptive cross-sectional quantitative study involving 384 participants from sample population aged 18 years and above who were mentally health and semi-structured questionnaires was used to collect data. The results 110 (28.8%) respondents have good knowledge toward mental illness, about 292(76%) have negative attitude towards mentally ill people, 92(24%) respondents have positive attitude toward people who are mentally ill. More over about 318 (82.9%) respondents agreed that care and support of family and friends, could help people with mental illness to get rehabilitation while 66(17.1%) respondents disagreed on the care and support of the family and friends could help mentally ill people to get rehabilitation; The findings show most have poor knowledge and negative attitudes towards people with mental illness and may impair their social reintegration in the community. There’s need to develop strategies to enlighten the public regarding nature of mental illness so as to foster acceptance of people with mental illness by the community members.
Model Latihan Passing Sepakbola Usia Sekolah Dasar
Authors: Bambang Triaji Asnoto  , Firmansyah Dlis  , Sri Nuraini
Published: October 2020

Tujuan penelitian ini adalah untuk menghasilkan model latihan passing sepakbola usia sekolah dasar. Subyek dalam penelitian dan pengembangan ini adalah siswa Usia Sekolah Dasar. Metode penelitian yang digunakan adalah model penelitian dan pengembangan dari Borg and Gall. Teknik pengumpulan data yang digunakan adalah hasil validasi pakar dan uji efektivitas melalui instrument penilaian dengan analisis data statistik uji-t. Hasil penelitian dan pengembangan model latihan passing sepakbola usia sekolah dasar menunjukkan bahwa (1) model latihan passing sepakbola usia sekolah dasar signifikan meningkatkan keterampilan teknik dasar passing sepakbola siswa, (2) Berdasarkan analisis data diperoleh nilai rata-rata pre test 14.38 dan rata-rata post test 20.03 standart deviasi pre test 2.272 dan post test 1.833 rata-rata pre test dan post test -5.650 dan standart deviasi 1.718 nilai t -20.800 taraf signifikasni 0,05 karena H0 ditolak dapat dikatakan bahwa model latihan passing sepakbola usia sekolah dasar dapat meningkatkan keterampilan teknik dasar passing serta efektif untuk diterapkan dalam proses latihan passing sepakbola pada usia sekolah dasar.

 

Kata Kunci: Model Latihan; Passing Sepakbola; Sekolah Dasar; Jurnal Olahraga; UNJ

 

 

Abstract

 

The research purpose is to produce a model of elementary school age football passing training. The subject in this research and development are elementary school age students. The research method using the model of Borg and Gall. Data collection techniques using the results of expert validation and trials through the Assessment instrument with t-test statistical data analysis. The results of research and development of elementary school age soccer practice models show that (1) the elementary school age football passing practice model that significantly increases the technical skills of passing soccer students, (2) Based on data analysis, the pre test average scores of 14.38 and the average post test 20.03 standard deviation of pre test 2,272 and post test 1,833 average pre test and post test -5,650 and standard deviation 1,718 value of t -20,800 significant level 0.05 because H0 was rejected, it can be said that the football passing practice model of elementary school age can improve basic passing technical skills and is effective to be applied in the process of passing football training at elementary school age.

 

Keywords: Exercise Model; Soccer Passing; Primary school; Sports Journal; UNJ
Upaya Meningkatkan Kemampuan Representasi Matematis Melalui Penerapan Model Problem Based Learning (PBL) Siswa Kelas X MIA 1 di SMAN 4 Bekasi
Authors: Jenita Gianthie , Sudaryati Sri , Ambarwati Lukita
Published: October 2020

Berdasarkan hasil observasi dan hasil tes awal kemampuan representasi matematis siswa di kelas X MIA 1 SMAN 4 Bekasi menunjukkan bahwa kemampuan representasi matematis siswa masih rendah, sehingga kemampuan tersebut perlu ditingkatkan. Dalam hal ini, model Problem Based Learning (PBL) dapat dijadikan salah satu alternatif dalam pelaksanan pembelajaran matematika di kelas. Model PBL terdiri dari lima fase yaitu orientasi siswa pada masalah, mengorganisasikan siswa untuk belajar, membimbing pengalaman siswa, mengembangkan dan menyajikan hasil karya, menganalisis dan mengevaluasi proses pemecahan masalah, masing-masing fase dapat meningkatkan kemampuan representasi matematis siswa dalam pembelajaran. Penelitian ini bertujuan untuk meningkatkan kemampuan representasi matematis siswa kelas X MIA 1 di SMAN 4 Bekasi melalui penerapan model PBL dalam pembelajaran. Penelitian ini merupakan penelitian tindakan kelas yang dilaksanakan dalam tiga siklus. Siswa diberikan kuis sebagai tes akhir pada setiap siklus untuk mengukur kemampuan representasi matematis siswa. Penelitian ini berlangsung dari bulan April hingga Mei 2016 di kelas X MIA 1 SMAN 4 Bekasi tahun ajaran 2015/2016 dengan jumlah siswa 40 orang. Hasil penelitian menunjukkan bahwa pembelajaran matematika menggunakan model PBL dapat meningkatkan kemampuan representasi matematis siswa. Hal tersebut ditunjukkan dengan adanya peningkatan rata-rata tes kemampuan representasi matematis dan peningkatan presentase jumlah siswa yang mencapai kriteria ketuntasan minimal (KKM) di setiap siklusnya.
Pengembangan Bahan Ajar Interaktif Berbasis Pendekatan Kontekstual Pada Materi Persamaan Garis Lurus Kelas VIII Sekolah Menengah Pertama
Authors: Wardatunnisa Nina , Ratnaningsih Ratnaningsih , Sudaryati Sri
Published: October 2020

Penelitian ini bertujuan untuk mengembangkan bahan ajar interaktif berbasis pendekatan kontekstual pada materi persamaan garis lurus kelas VIII Sekolah Menengah Pertama. Berdasarkan analisis kebutuhan, materi dalam bahan ajar yang dikembangkan adalah persamaan garis lurus menggunakan pendekatan kontekstual. Pendekatan kontekstual terdiri atas tujuh asas yaitu, konstruktivisme (constructivism), inkuiri (inquiry), bertanya (questioning), masyarakat belajar (learning community), memodelan (modelling), refleksi (reflection), dan penilaian nyata (authentic assesment). Ketujuh asas tersebut terdapat pada bagian-bagian di dalam bahan ajar.Metode yang digunakan pada penelitian ini adalah penelitian dan pengembangan (research and development). Prosedur penelitian dan pengembangan ini terdiri dari enam tahap, yaitu analisis kebutuhan, pengembangan produk awal, validasi ahli dan revisi, uji coba perorangan dan revisi, uji coba lapangan skala kecil dan revisi, dan uji coba skala besar dan produk akhir.Berdasarkan hasil validasi ahli materi dan bahasa, diperoleh persentase sebesar 89,40% dengan kategori sangat baik. Bahan ajar yang dikembangkan sesuai dengan kurikulum yang berlaku dan bahan ajar sesuai dengan asas pendekatan kontekstual. Berdasarkan hasil validasi ahli media, diperoleh persentase 82,90% dengan kategori sangat baik. Bahan ajar yang dikembangkan interaktif dan memunculkan motivasi untuk belajar. Berdasarkan hasil uji coba perorangan, diperoleh persentase sebesar 84,70% dengan kategori sangat baik. Persentase yang diperoleh dari uji coba lapangan skala kecil yaitu 88,30% dengan kategori sangat baik. Begitu juga dengan uji coba skala besar, diperoleh kategori sangat baik dengan persentase sebesar 90,50%. Berdasarkan hasil uji coba tersebut, dapat dikatakan bahwa bahan ajar matematika yang dikembangkan memenuhi kelayakan untuk digunakan pada pembelajaran materi persamaan garis lurus kelas VIII Sekolah Menengah Pertama.
Mengembangkan Kemampuan Penalaran Induktif dengan Model-Eliciting Activities pada Pembelajaran Remedial Pola Bilangan untuk Kelas VIII E SMP Negeri 22 Jakarta
Authors: Khairunnisa Fitri , Wardani Rahayu  , Meiliasari
Published: October 2020

Penelitian-penelitian terdahulu menunjukkan bahwa peserta didik kelas VIII mengalami kesulitan dalam menentukan output suatu pola bilangan apabila diberikan input yang besar dan menentukan rumus umum Un. Hal serupa juga terjadi pada siswa kelas VIII SMP Negeri 22 Jakarta. Sebanyak lebih dari 50% peserta didik kelas VIII E SMP Negeri 22 Jakarta mendapat hasil Penilaian Harian (PH) di bawah Kriteria Ketuntasan Minimal (KKM). Menentukan rumus umum Un adalah kesulitan yang paling sering dijumpai oleh peserta didik. Menentukan rumus umum Un merupakan salah satu indikator kemampuan penalaran induktif. Maka, kemampuan penalaran induktif peserta didik kelas VIII E SMP Negeri 22 Jakarta masih lemah. Hal inilah yang mendasari peneliti untuk merancang teori pembelajaran lokal dalam pembelajaran remedial pada pokok bahasan Pola Bilangan dengan menggunakan Model-Eliciting Activities (MEAs) untuk meningkatkan kemampuan penalaran induktif peserta didik kelas VIII E SMP Negeri 22 Jakarta. Metodologi penelitian yang digunakan adalah design research, yang dimulai dari preliminary design, teaching experiment, dan retrospective analysis. Hasil penelitian menunjukkan bahwa MEAs dapat meningkatkan kemampuan penalaran induktif peserta didik dalam pembelajaran remedial pada pokok bahasan Pola Bilangan.
Barriers and opportunities for cleanliness of shared sanitation facilities in low-income settlements in Kenya
Authors: Simiyu Sheillah , Kweyu Raphael M. , Antwi-Agyei Prince , Adjei Kwaku A.
Published: October 2020

Background: The sharing of sanitation facilities is a common practice in low-income areas in sub-Saharan Africa. However, shared sanitation is currently categorized as a limited sanitation service, and may therefore not count towards meeting the global goals. These shared facilities are often the only option available for most residents in low-income settlements, and improving their cleanliness and overall management is key to reducing open defecation and risk of disease. This study sought to investigate barriers and opportunities for improved cleanliness of shared sanitation facilities in low-income settlements of Kisumu city, Kenya. Methods: Thirty-nine in-depth interviews and 11 focus group discussions were held with residents – mainly tenants and landlords – of a low-income settlement in Kisumu. Analysis followed a thematic approach to define the problem, specify the target behaviour and identify the changes needed. Results: Sanitation facilities were mainly pit latrines, typically shared among landlords and tenants. Participants singled out behavioural (poor use of the shared toilets) and social (lack of cooperation in cleaning) challenges that led to unclean shared toilets. Available opportunities for improvement included instituting clear cleaning plans, improving communication among users, and enhanced problem-solving mechanisms between landlords and tenants. These approaches could form the basis for designing intervention strategies for improving the cleanliness of shared sanitation facilities. Conclusion: The results highlight the need to focus on social aspects for improvement of cleanliness in shared sanitation facilities in low-income settlements. Through a social approach, shared sanitation facilities can be managed appropriately to provide the millions of low-income residents in Kenya an opportunity to access sanitation. This study provides further evidence on approaches for improved management of shared sanitation facilities in line with the World Health Organization’s (WHO) Joint Monitoring Program’s (JMP) recommendation for high quality shared facilities
Evaluation of the diagnostic accuracy of a new point-of-care rapid test for SARS-CoV-2 virus detection
Authors: Miscio Leonardo , Olivieri Antonio , Labonia Francesco , Feo Gianfranco De , Chiodini Paolo , Portella Giuseppe , Atripaldi Luigi , Parrella Roberto , Conenna Rodolfo , Buonaguro Franco Maria , Cavalcanti Ernesta , Ascierto Paolo , Botti Gerardo , Bianchi Attilio
Published: October 2020

Background: The easy access to a quick diagnosis of coronavirus disease 2019 (COVID-19) is a key point to improve the management of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to contain its spread. Up to now, laboratory real-time PCR is the standard of care, but requires a fully equipped laboratory and significant infrastructure. Consequently, new diagnostic tools are required. Methods: In the present work, the diagnostic accuracy of the point-of-care rapid test "bKIT Virus Finder COVID-19" (Hyris Ltd) is evaluated by a retrospective and a prospective analysis on SARS CoV-2 samples previously assessed with an FDA “authorized for the emergency use - EUA” reference method. Descriptive statistics were used for the present study.Results: Results obtained with the Hyris Kit are the same as that of standard laboratory-based real time PCR methods for all the analyzed samples. In addition, the Hyris Kit provides the test results in less than 2 hours, a significantly shorter time compared to the reference methods, without the need of a fully equipped laboratory. Conclusions: To conclude, the Hyris kit represents a promising tool to improve the health surveillance and to increase the capacity of SARS-CoV-2 testing.
Small Business and Their Challenges During COVID-19 Pandemic in Developing Countries; in Case of Ethiopia
Authors: Engidaw Abriham Ebabu
Published: October 2020

The main objective of this study was to demonstrate the challenges of small businesses during corona virus pandemic in developing countries specifically in Ethiopia. Methodologically, the study deployed secondary data analysis followed descriptive research design. Also, this study was conducted through both qualitative and quantitative research approach. The secondary data analysis result shows that many small and large businesses are suffering challenges and this unprecedented coronavirus crisis has caused destruction for many businesses in the globe and it is challenging to survive with reduced revenue, jobs lost and life slowing down and weak marketing performance even difficult to keep a calm head and their business alive. To support these businesses the government should use different mechanisms by cooperating with wealthy peoples and other non-governmental organizations. Besides, the small business owners should manage expectations and communicate with staff, suppliers, banks, and customers throughout this coronavirus frightening. Reduce expenses, be open to their employees about their finances and keep marketing, use different alternatives to deliver their product and recover from the crisis.
Myeloid cell dynamics correlate with clinical outcomes of severe coronavirus disease 2019
Authors: Takano Tomohiro , Matsumura Takayuki , Adachi Yu , Terahara Kazutaka , Moriyama Saya , Onodera Taishi , Nishiyama Ayae , Kawana-Tachikawa Ai , Miki Shoji , Hosoya-Nakayama Kaori , Nakamura-Hoshi Midori , Seki Sayuri , Tachikawa Natsuo , Yoshimura Yukihiro , Miyata Nobuyuki , Horiuchi Hiroshi , Sasaki Hiroaki , Miyazaki Kazuhito , Kinoshita Noriko , Sudo Tsutomu , Akiyama Yutaro , Sato Rubuna , Suzuki Tadaki , Matano Tetsuro , Takahashi Yoshimasa
Published: October 2020

An expanded myeloid cell compartment is a hallmark of severe coronavirus disease 2019 (COVID-19); however, it remains unclear whether myeloid cells are beneficial or detrimental to the clinical outcome. Here, we tracked cellular dynamics of myeloid-derived suppressor cell (MDSC) subsets and examined whether any of them correlate with disease severity and prognosis by flow cytometric analysis of blood samples from COVID-19 patients. We observed that polymorphonuclear (PMN)-MDSCs, rather than other MDSC subsets, transiently expanded in severe cases but not in mild or moderate cases. Notably, this subset was selectively expanded in survivors of severe cases and diminished during recovery. Analysis of plasma cytokines/chemokines revealed that interleukin-8 increased prior to PMN-MDSC expansion in survivors and returned to basal levels during the recovery phase. In contrast, interleukin-6 and interferon-g-induced protein 10 were abundantly induced in non-survivors, suggesting possible downstream targets for the immunosuppressive effects of the MDSC subset. Our data indicate that increased cellularity of PMN-MDSCs might be beneficial for the clinical outcome and could be useful as a possible predictor of prognosis in cases of severe COVID-19.
A Blockchain and FOT (Fog of Things) based system and method for predicting an Infectious disease transmitted by a virulent respiratory virus
Authors: Tewari Naveen , Kumar Rajeev
Published: October 2020

The present research is generally related to a system and method for predicting an infectious disease such as COVID-19 transmitted by a virulent respiratory virus. Disclosed are a system and method for predicting an infectious disease transmitted by a virulent respiratory virus. The system includes a plurality of Internet of Things (IoT) sensors, a plurality of fog node devices, and a plurality of computing devices in cloud data centers. The IoT sensors are configured to be coupled to a plurality of human beings to generate a health dataset. The fog node devices are associated with a fog layer to receive the health dataset from the IoT sensors to process and to store the health dataset over a blockchain network. The fog node devices process the health dataset at the fog layer by performing a fog computing. The computing devices and cloud data centers receive the processed health dataset from the of fog node devices over the blockchain network. This research is also filed for patent in Indian Patent Office with application number – 202011021969.
Correlates of knowledge of family planning among people living in fishing communities of Lake Victoria, Uganda
Authors: Nanvubya Annet , Wanyenze R.K. , Nakaweesa T. , Mpendo J. , Kawoozo B. , Matovu F. , Nabukalu S. , Omoding G. , Kaweesi J. , Ndugga J. , Kamacooko O. , Chinyenze K. , Price M. A. , Geertruyden J.P. Van
Published: October 2020

Background: Knowledge of family planning (FP) is a key determinant of contraceptive use which ultimately plays a role in attainment of good health and in conduct of clinical research. People living in fishing communities (FCs) have limited access to health services including FP and are targeted for future clinical research but their knowledge of FP and its correlates are scantily known. We determined correlates of knowledge of FP among people living in FCs of L. Victoria in Uganda to inform future FP education programs in FCs. Methods: We conducted a comparative cross-sectional survey among participants aged 15-49 years from Kigungu and Nsazi. Participants were asked if they were aware of any FP method. All those who responded in the affirmative were further asked to mention what FP methods they had heard of or knew. Those who reported knowledge of at least one FP method were asked a series of questions about FP methods and their side effects. Knowledge was categorized into good or poor knowledge based on their mean total score. Poor knowledge constituted a score below the mean while good knowledge constituted a score of more than or equal to the mean total score. To further explore attitudes and perceptions of FP, ten in-depth interviews and four focus group discussions were conducted. Results: Of the 1,410 screened participants, 94.5% were aware of at least one FP method. Pills and injectable hormonal methods were the most commonly known methods. Slightly over a third (38%) had good knowledge of FP. Correlates of knowledge of FP were; being female (aOR: 1.92 95% CI: 1.39-2.67), residing in Kigungu (aOR: 4.01 95% CI: 2.77-5.81), being married (aOR: 1.59 95% CI: 1.11-2.28) and currently being in a sexual relationship (aOR: 1.75 95% CI: 1.18-2.60). Concerns about safety and effectiveness of some modern FP methods exist. Misconceptions on effects of FP like sterility, cancers and foetal abnormalities were common. Conclusion: FP awareness among people living in FCs of L. Victoria in Uganda is high. However, good knowledge about specific methods tends to be low. Correlates of knowledge of FP include gender, residence, marital status and sexual engagement.
Self-perceived Workplace Discrimination and Mental Health Among Immigrant Workers in Italy: a Cross-sectional Study
Authors: Napoli Anteo Di , Rossi Alessandra , Baralla Francesca , Ventura Martina , Gatta Rosaria , Perez Monica , Sarchiapone Marco , Mirisola Concetta , Petrelli Alessio
Published: October 2020

Background: The process of immigration is associated with poor mental and physical health. While the workplace represents an important context of social integration, previous studies evaluating the effect of discrimination experienced in the workplace found worse mental health status among immigrants. The aim of this study was to investigate whether self-perceived workplace discrimination has any role in the mental health status of immigrants living and working in Italy, evaluating the contribution of other personal experiences, such as loneliness and life satisfaction.Methods: A cross-sectional study was conducted on a sample of 12,408 immigrants (aged 15-64) living and working in Italy. Data derived from the first national survey on immigrants carried out by the Italian National Institute of Statistics (Istat). Mental health status was measured through the Mental Component Summary (MCS) of the SF-12 questionnaire. A linear multivariate linear regression was carried out to evaluate the association between mental health status, self-perceived workplace discrimination, and sociodemographic factors; a structural equation model was used to test hypothesized relationships shown in a conceptual path analysis model.Results: Worse mental health status was independently (p<0.001) associated with self-perceived workplace discrimination (β:-1.873), self-perceived loneliness (β:-3.021), level of life satisfaction (β:-2.775), and physical health status (β:-0.088). As confirmed by the structural equation model, the effect of self-perceived workplace discrimination on MCS was mediated by the other factors considered: self-perceived loneliness (13.6%), level of life satisfaction (13.6%), and physical health status (3.9%).Conclusions: Our study suggests that self-perceived workplace discrimination is associated with worse mental health status in immigrant workers through personal experiences in the workplace and explains the effect of the exposure to workplace discrimination on immigrants’ psychological well-being. Our findings suggest that an overall public health response is needed to facilitate the social integration of immigrants and their access to health services, particularly those services that address mental health issues.
Intra-tumoral genomic heterogeneity in rectal cancer: mutational status is dependent on preoperative biopsy depth and location.  
Authors: Vuijk Floris A. , de Water Carlijn van , Vliet Shannon van Lent - van , Valk Maxime J.M. van der , Simmer Femke , de Velde Cornelis J.H. van , Vahrmeijer Alexander L. , Nagtegaal Iris D. , Hilling Denise E.
Published: October 2020

BackgroundNeoadjuvant therapy before surgical resection is indicated for patients with locally advanced rectal cancer. However, a significant number of patients show minimal or no response to neoadjuvant therapy. Unfortunately, we are currently unable to predict response and identify non-responding patients before neoadjuvant treatment is given. Genomic mutational status might provide valuable prognostic information. However, it is unclear whether predictions based on genomic mutational status in single preoperative biopsies are reliable due to intra-tumoral heterogeneity. In this study we aim to investigate the reliability of genomic mutations found in single pre-operative biopsies by comparing these genomic mutations to 4 other locations within the same tumor using next generation sequencing.Methods Rectal cancer patients undergoing primary resection, without neoadjuvant therapy, were included. Of all patients, one biopsy, two deep and two superficial samples were obtained and sequenced using a targeted next generation sequencing gene panel. Concordance between these 5 samples was assessed.Results In this feasibility study we included 11 patients. In 7 out of 11 (64%) patients, all 5 samples showed concordant mutations. In 4 out of 11 patients (36%) discordant mutations were observed. ConclusionsIn conclusion, assessment of mutational status on a single pre-operative biopsy shows discordance with tumor tissue from other locations in 36% of cases. These results warrant careful interpretation of biopsy material analysis, as these might be influenced by tumor heterogeneity.
Risk Factors For Severity Of Thrombocytopenia In Full Term Infants: A Single Center Study
Authors: Saber Amira M , Aziz Shereen P , Almasry Al Zahraa E , Mahmoud Ramadan A
Published: October 2020

Background: Neonatal thrombocytopenia (NT) (platelet count <150 x 109/L) is a common finding in the neonatal intensive care unit (NICU). The main aim of this study was to assess the prevalence, rick factors, and outcomes of severe NT in full term (FT) infants.Methods: During the study period, all FT infants who met the inclusion criteria for NT on two occasions were included. Maternal data, such as maternal age, weight, gestational age, mode of delivery, and history of systemic diseases, including diabetes mellitus, pre-eclampsia, systemic lupus erythematosus, and immune thrombocytopenic purpura, were recorded. Furthermore, neonatal data, such as gender, neonatal weight, causes/duration of admission, types of respiratory support used, complete blood count measurements, and outcomes for neonates admitted to the NICU, were recorded.Results: In total, 55 FT infants with NT met the inclusion criteria, and 29 (52.73%) cases had severe NT. The most common cause of NT was neonatal sepsis (20 cases, 36.35%), followed by a postoperative state (5 cases, 9.09%). Moreover, in cases of positive blood cultures, the most commonly isolated organism was Escherichia coli (6 cases, 10.90%), followed by Klebsiella (5 cases, 9.09%). Cases of severe NT needed more platelet transfusions (P=0.001) and had higher rates of mortality (P=0.001) when compared to cases of mild/moderate NT associated with signs of bleeding and pulmonary/intraventricular hemorrhage (IVH) (P=0.001).Conclusion: Thrombocytopenia is a frequent challenge between neonatologists. The most common causes of NT in FT infants were neonatal sepsis, followed by postoperative state. Furthermore, when severe NT compared to mild/moderate NT, associated with signs of bleeding and pulmonary/IVH, needed more platelet transfusions, and had increased mortality. Further research is needed to explain which of these complications related to severity of thrombocytopenia or were associated with the bad general condition of these patients due to their original disease.
Lotus leaf extract inhibits ER- breast cancer cell migration and metastasis
Authors: Tong yuelin , Li Zhongwei , Wu Yikuan , Zhu Shenglong , Lu Keke , he zhao
Published: October 2020

Background: Patients with estrogen receptor negative (ER-) breast cancer have poor prognosis due to high rates of metastasis. However, there is no effective treatment and drugs for ER- breast cancer metastasis. Our purpose of this study was to evaluate the effect of lotus leaf alcohol extract (LAE) on the cell migration and metastasis of ER- breast cancer.Methods: The anti-migratory effect of LAE were analyzed in ER- breast cancer cells including SK-BR-3, MDA-MB-231 and HCC1806 cell lines. Cell viability assay, wound-healing assay, RNA-sequence analysis and immunoblotting assay were used to evaluate the cytotoxicity and anti-migratory effect of LAE. To further investigate the inhibitory effect of LAE on metastasis in vivo, subcutaneous xenograft and intravenous injection nude mice models were established. Lung and liver tissues were analyzed by the Hematoxylin & eosin staining and immunoblotting assay. Results: We found that lotus leaf alcohol extract (LAE), not nuciferine, inhibited cell migration significantly in SK-BR-3, MDA-MB-231 and HCC1806 breast cancer cells, and did not affect viability of breast cancer cells. The anti-migratory effect of LAE was dependent on TGF-β1 signaling, while independent of Wnt signaling and autophagy influx. Intracellular H2O2 was involved in the TGF-β1-related inhibition of cell migration. LAE inhibited significantly the breast cancer cells metastasis in mice models. RNA-sequence analysis showed that extracellular matrix signaling pathways are associated with LAE-suppressed cell migration. Conclusions: Our findings demonstrated that lotus leaf alcohol extract inhibits the cell migration and metastasis of ER- breast cancer, at least in part, via TGF-β1/Erk1/2 and TGF-β1/SMAD3 signaling pathways, which provides a potential therapeutic strategy for ER- breast cancer.
Quality of sleep and associated factors among people living with HIV/AIDS on follow up at Ethiopian Zewditu Memorial Hospital,2018
Authors: Mengistu Nebiyu , Azale Telake , Yimer Solomon , Fikreyesus Mahilet , Melaku Elsa , Shumye Seid
Published: October 2020

Background: Sleep disturbance is a common complaint in people living with HIV/AIDS. Despite the influence of sleep disturbance on treatment adherence, quality of life, work productivity, risk of chronic illness, it remains under-recognized and under-studied in Ethiopia. Therefore it is necessary to produce scientific evidence to fill the knowledge gap and areas of management. The current study aimed to assess sleep quality and its associated factors among people living with HIV/AIDS. Methods: An institution-based cross-sectional study was utilized among 408 participants who were selected by a systematic random sampling technique at Zewditu memorial hospital from April to May 2018. The Pittsburgh Sleep Quality Index questionnaire was used to measure sleep quality. Ethical clearance was obtained from the joint ethics committee of the University of Gondar and Amanuel Mental Specialized Hospital. Oral informed consent was obtained from each participant. Binary and multivariable logistic regression models were fitted. Odds ratios (OR) with the corresponding 95% confidence interval (95%CI) was computed. Results: The magnitude of poor sleep quality was 55.6%. Being female [AOR=3.40, 95% CI: (1.80, 6.41)], depression [AOR =3.52, 95% CI: (1.95, 6.32)], CD4count ≤ 200 cells/mm3 [AOR=3.18,95%CI: (1.65,6.13)], duration of HIV/AIDS diagnosis [AOR=3.43,95% CI: (1.61,7.29)], current use of tobacco [AOR=5.69, 95% CI: (2.04,15.9)] and chat or caffeinated drinks [AOR=2.65, 95% CI: (1.06,6.64)] and poor sleep hygiene [AOR=3.55, 95% CI: (1.85, 6.78)] were significantly associated with poor sleep quality.Conclusions: More than half of the study participants were found to have poor sleep quality. A range factors influence quality of sleep of people with HIV/AIDS. Routine screening of sleep condition among people living with HIV/AIDS and early intervention based on the findings is suggested.
Clinical features and neuropsychiatric comorbidities in pediatric patients with tic disorders: A retrospective chart review study from South Korea
Authors: Park Eu Gene , Kim Young-Hoon
Published: October 2020

Background: Tic disorders are childhood-onset neuropsychiatric disorders characterized by multiple motor or vocal tics with frequent comorbidities and a broad spectrum of phenotypic presentations. In this study, we aimed to investigate the clinical characteristics and comorbid neuropsychiatric conditions in pediatric patients with tic disorders. Methods: We retrospectively reviewed the medical records of 119 pediatric patients (89 males, 30 females) who were diagnosed with tic disorders according to the Diagnostic and Statistical Manual of Mental Disorders, 5th edition (DSM-5) at Uijeongbu St. Mary’s Hospital, Republic of Korea, between January 2012 and July 2019.Results: The mean age of tic onset was 6.9 years (range, 1–14) and the mean age at diagnosis was 8 years (range, 1–17). The mean lag between tic onset and diagnosis was 13.3 months (range, 0.25–132). The most common, first-presenting tics were eye blinking (50.4%), followed by jaw or lip movement (29.4%) and throat clearing (29.4%). Thirty-seven (31.1%) patients had at least one co-occurring neuropsychiatric disorder at the time of tic diagnosis. Subtypes of tic disorders, types of initial tics, and presence of neuropsychiatric comorbidities were not associated with tic severity. Tic severity was associated with greater functional impairment and tic noticeability (p <0.05). A relatively shorter time to diagnosis was associated with tic severity (Spearman’s ρ = –0.14, p = 0.11).Conclusions: The evolving nature of tic expression and severity, high prevalence of neuropsychiatric comorbidities, and associated functional impairments emphasize the importance of comprehensive assessment during the disease course for determining and prioritizing goals of treatment.
Dysphagia and Masticatory Performance as a Mediator of the Xerostomia to Quality of Life Relation in the Older Population
Authors: Lu Ting-Yu , Chen Jen-Hao , Du Je-Kan , Lin Ying-Chun , Ho Pei-Shan , Lee Chien-Hung , Hu Chih-yang , Huang Hsiao-Ling
Published: October 2020

Background: The impact of poor oral health on older adults’ quality of life is a public health problem. In this study, the mediating effects of dental status, occlusal condition, dysphagia, and masticatory performance on the association between xerostomia and oral health-related quality of life (OHRQoL) were assessed in the older adult population. Methods: Stratified cluster sampling was used to recuit 1076 community-dwelling adults aged 65 years and older from Kaohsiung, Taiwan. Community care centers were randomly selected according to their geographic classifications (urban, rural, or mountainous areas). Assessments of dental status and occlusal condition were performed by dentists. Information on demographics, physical function, xerostomia, dysphagia and depression was collected through face-to-face interviews. Masticatory performance was evaluated using color-changeable chewing gum. OHRQoL was measured using the Geriatric Oral Health Assessment Index. Hierarchical regression models were used to assess the relationships between OHRQoL and physical function, dental status and oral function in older adults. Path analysis was used to estimate direct and indirect pathways between xerostomia and OHRQoL.Results: Participants with xerostomia exhibited a 0.20 OHRQoL reduction (p < .001) compared with patients with no xerostomia, and the direct effect accounted for 83.3% of the total effect. Dysphagia and masticatory performance were found to exert significant mediating effects on the association between xerostomia and OHRQoL (βs = 0.20 and −0.12, respectively; both p < .001; βs = 0.06 and −0.09, respectively; both p < .05). Moreover, potential mediating effects of the number of functional teeth (βs = −0.11 and −0.43, respectively; both p < .001) and occlusal condition (βs = 0.09 and 0.13, respectively; both p < .05) on the relationship between xerostomia and masticatory performance were noted.Conclusions: Dysphagia and masticatory performance may serve as pathways through which xerostomia affects quality of life. Early oral function intervention may be a valuable and actionable target for older adults to maintain quality of life. Our results further suggest that checkup and screening for oral dysfunction are essential to prevent or delay the onset of complications.
Cangrelor ameliorates CLP-induced pulmonary injury in sepsis by inhibiting GPR17
Authors: Luo Qiancheng , Liu Rui , Liu Guorong , Hang Min , Chen Guo , Xu Lei , Jin Qinqin , Guo Dongfeng , Kang Qi
Published: October 2020

Background: Sepsis is a common complication of severe wound injury and infection, with a very high mortality rate. The P2Y12 receptor inhibitor, cangrelor, is an antagonist antiplatelet drug.Methods: In our study, we investigated the protective mechanisms of cangrelor in CLP-induced pulmonary injury in sepsis, using C57BL/6 mouse models.Results: TdT-mediated dUTP Nick-End Labeling (TUNEL) and Masson staining showed that apoptosis and fibrosis in lungs were alleviated by cangrelor treatment. Cangrelor significantly promoted surface expression of CD40L on platelets and inhibited CLP-induced neutrophils in Bronchoalveolar lavage fluid (BALF) (p＜0.001). We also found that cangrelor decreased the inflammatory response in the CLP mouse model and inhibited the expression of inflammatory cytokines, IL-1β (p＜0.01), IL-6 (p＜0.05), and TNF-α (p＜0.001). Western blotting and RT-PCR showed that cangrelor inhibited the increased levels of G-protein-coupled receptor 17 (GPR17) induced by CLP(p＜0.001). Conclusion: Our study indicated that cangrelor repressed the levels of GPR17, followed by a decrease in the inflammatory response and a rise of neutrophils in BALF, potentially reversing CLP-mediated pulmonary injury during sepsis.
Continuous Endometrial Volumetric Analysis for Endometrial Receptivity Assessment on Assisted Reproductive Technology Cycles
Authors: Martins Renato , Oliani Antonio Helio , Oliani Denise Vaz , de Oliveira Jose Martinez
Published: October 2020

Background: Human implantation is a complex process requiring synchrony between a healthy embryo and a functionally competent or receptive endometrium. In order to assess endometrial receptivity in Assisted Reproductive Technology (ART) cycles serial evaluation of endometrial volumetric analysis may have a predictive value on a positive outcome. Methods: Serial 3D transvaginal ultrasound performed in women on ART cycle to evaluate embryo implantation predictors. Prospective case control study of 169 subjects were assessed. Endometrial pattern, thickness, volume and adjusted endometrial volume (ratio between endometrial volume and uterine volume) was performed to all subjects on a continuous process from baseline, during controlled ovarian stimulation, trigger day with human chorionic gonadotropin hormone (hCG) and at embryo transfer day. Results: Demographics and ART procedures and scores, was similar between the two groups. Endometrial morphology also showed no difference between the two groups. Endometrial volume and adjusted endometrial volume was significantly higher in the positive group as soon as day 6 of ovarian controlled stimulation. Conclusions: Serial 3D endometrial volume and adjusted endometrial volumes provides a predicting clinical tool enhancing elective embryo transfers in fresh ART cycle. Thus providing a non-invasive continuous technique for endometrial receptivity assessment that reflects endometrial changes during ART procedures.
Barriers to women's disclosure of Domestic Violence in health services in Palestine: qualitative interview-based study
Authors: Shaheen Amira , ashkar suzy , alkaiyat abdulsalam , bacchus loraine , colombini manuela , feder gene , evans maggie
Published: October 2020

Background: Domestic violence (DV) damages health and requires a global public health response and engagement of clinical services. Recent surveys show that 27% of married Palestinian women experienced some form of violence from their husbands over a 12 month’s period, but only 5% had sought formal help, and rarely from health services. Across the globe, barriers to disclosure of DV have been recorded, including self-blame, fear of the consequences and lack of knowledge of services. This is the first qualitative study to address barriers to disclosure within health services for Palestinian women. Methods: In-depth interviews were carried out with 20 women who had experienced DV. They were recruited from a non-governmental organisation offering social and legal support. Interviews were recorded, transcribed and translated into English and the data were analysed thematically. Results: Women encountered barriers at individual, health care service and societal levels. Lack of knowledge of available services, concern about the health care primary focus on physical issues, lack of privacy in health consultations, lack of trust in confidentiality, fear of being labelled ‘mentally ill’ and losing access to their children were all highlighted. Women wished for health professionals to take the initiative in enquiring about DV. Wider issues concerned women’s social and economic dependency on their husbands which led to fears about transgressing social and cultural norms by speaking out. Women feared being blamed and ostracised by family members and others, or experiencing an escalation of violence. Conclusions: Palestinian women’s agency to be proactive in help-seeking for DV is clearly limited. Our findings can inform training of health professionals in Palestine to address these barriers, to increase awareness of the link between DV and many common presentations such as depression, to ask sensitively about DV in private, reassure women about confidentiality, and increase awareness among women of the role that health services can play in DV.
WITHDRAWN: The lncRNA XIST promotes colorectal cancer cell growth through regulating the miR-497-5p/FOXK1 axis
Authors: Wang Nan , He Jia-Xing , Jia Guo-Zhan , Wang Ke , Zhou Shuai , Wu Tao , He Xian-Li
Published: October 2020

Background: Recent studies suggest that long noncoding RNAs (lncRNAs) play an important role in tumorigenesis. As a newly identified lncRNA, the role of XIST in colorectal cancer (CRC) has not been established. Here, we sought to characterize the role of XIST and its associated regulatory network in CRC cells. Methods: Expression of XIST mRNA, miR-497-5p, and forkhead box k1 (FOXK1) in CRC cells and tissues were detected using quantitative real-time polymerase chain reaction (qRT-PCR). Proliferation and apoptosis of CRC cells were determined using the CCK-8 cell counting assay and flow cytometry. The rate of cell migration and invasion was determined using a transwell assay. The relationships between XIST, miR-497-5p, and FOXK1 were predicted and confirmed using a dual-luciferase reporter assay. Expression of FOXK1 protein was quantified by Western blot. Results: XIST and FOXK1 expression were significantly upregulated in CRC tissues and cell lines, while miR-497-5p expression was downregulated. XIST knockdown significantly suppressed CRC cell proliferation, migration, and invasion. Silencing of XIST also reversed the downregulation of miR-497-5p and upregulation of FOXK1. Moreover, blocking XIST expression was shown to inhibit CRC tumor growth in vivo and the effects were antagonized by the loss of miR-497-5p. miR-497-5p was shown to act as a sponge of XIST and also targeted FOXK1 in CRC cells.Conclusion: XIST was shown to promote the malignancy of CRC cells by competitively binding to miR-497-5p, resulting in an increase in FOXK1 expression. These results suggest that targeting of XIST may represent a possible treatment for colon cancer.
Impact of daily versus weekly service of infectious diseases consultation on hospital antimicrobial consumption: a retrospective study
Authors: Cona Andrea , Gazzola Lidia , Viganò Ottavia , Bini Teresa , Marchetti Giulia Carla , Monforte Antonella d'Arminio
Published: October 2020

Background: To verify whether a daily service of Infectious Diseases consultation (ID-cons) is more effective than a weekly service in reducing antibiotic (ATB) consumption without worsening of clinical outcomes. Methods: Two-year observational analysis of the ID-cons provided in a hospital setting in Milan, Italy. ID-cons resulted in: start-of-ATB; no-ATB; confirmation; modification-of-ATB. The impact of a weekly (September 1, 2016 - August 31, 2017) versus a daily (September 1, 2017 – September 30, 2018) service of ID-cons was evaluated in terms of: time-from-admission-to-first-ID-cons, type of ATB-intervention and number-of-ID-cons per 100 bed-days (bd). Primary outcomes: reduction of hospital ATB consumption overall and by department and classes expressed as Defined Daily Dose (DDD)/100bd (by Wilcoxon test for paired data). Secondary outcomes: overall and sepsis-related in-hospital annual mortality rates (as death/patient’s admissions). Results: Overall 2552 ID-cons in 1111 patients (mean, 2.3 ID-cons per patient) were performed (18.6% weekly vs 81.4% daily). No differences in patient characteristics were observed. In the daily-service, compared to the weekly-service, patients were seen by the ID-consultant earlier (time-from-admission-to-ID-cons: 6 days (IQR 2-13) vs 10 days (IQR 6-19), p<0.001) and ATB was more often started by the ID-consultant (Start-of-ATB: 11.6% vs 8%, p=0.02), rather than treating physicians. After switching to daily-service, the number-of-ID-cons increased from 0.4/100bd to 1.5/100bd (p=0.01), with the greatest increase in the emergency department (1.5/100bd vs 6.7/100bd, p<0.001). Total ATB consumption decreased from 64 to 60 DDD/100bd. As for the number-of-cons, the consumption of ATB decreased mainly in the emergency area. According to ATB classes, glycopeptides consumption was reduced from 3.1 to 2.1 DDD/100bd (p=0.02) while carbapenem use decreased from 3.7 to 3.1 DDD/100bd (p=0.07). No changes in overall mortality (5.2% vs 5.2%) and sepsis-related mortality (19.3% vs 20.9%; p=0.7) were observed among the two time-period. Conclusions: Daily-ID-cons resulted in a more comprehensive management of the infected patient by the ID-consultant, especially in the emergency area where we also observed the highest rate of reduction of ATB-usage. No change in mortality was observed.