bluuebunny/crossref_metadata_2025 huggingface dataset:
Glycosylation of IgG Associates with Hypertension and Type 2 Diabetes Mellitus Comorbidity in the Chinese Muslim Ethnic Minorities and the Han Chinese
Authors: Meng Xiaoni , Song Manshu , Vilaj Marija , Štambuk Jerko , Dolikun Mamatyusupu , Zhang Jie , Liu Di , Wang Hao , Zhang Xiaoyu , Zhang Jinxia , Cao Weijie , Momčilović Ana , Trbojević-Akmačić Irena , Li Xingang , Zheng Deqiang , Wu Lijuan , Guo Xiuhua , Wang Youxin , Lauc Gordan , Wang Wei
Published: June 2021

Objectives: Hypertension and type 2 diabetes mellitus comorbidity (HDC) is common, which confers a higher risk of cardiovascular disease than the presence of either condition alone. Describing the underlying glycomic changes of immunoglobulin G (IgG) that predispose individuals to HDC may help develop novel protective immune-targeted and anti-inflammatory therapies. Therefore, we investigated glycosylation changes of IgG associated with HDC. Methods: The IgG N-glycan profiles of 883 plasma samples from the three northwestern Chinese Muslim ethnic minorities and the Han Chinese were analyzed by ultra-performance liquid chromatography instrument. Results: We found that 12 and six IgG N-glycan traits showed significant associations with HDC in the Chinese Muslim ethnic minorities and the Han Chinese, respectively, after adjustment for potential confounders and false discovery rate. Adding the IgG N-glycan traits to the baseline models, the area under the receiver operating characteristic curves (AUCs) of the combined models differentiating HDC from hypertension (HTN), type 2 diabetes mellitus (T2DM), and healthy individuals were 0.717, 0.747, and 0.786 in the pooled samples of Chinese Muslim ethnic minorities, and 0.828, 0.689, and 0.901 in the Han Chinese, respectively, showing improved discriminating performance than both the baseline models and the glycan-based models. Conclusion: Altered IgG N-glycan profiles were shown to associate with HDC, suggesting the involvement of inflammatory processes of IgG glycosylation. The alterations of IgG N-glycome, illustrated here for the first time in HDC, demonstrate a biomarker potential, which may shed light on future studies investigating their potential for monitoring or preventing the progression from HTN or T2DM towards HDC.
The Impact of Marijuana on Antidepressant Treatment in Adolescents: Clinical and Pharmacologic Considerations
Authors: Vaughn Samuel , Strawn Jeffrey , Poweleit Ethan , Sarangdhar Mayur , Ramsey Laura
Published: June 2021

The neuropharmacology of marijuana, including its effects on selective serotonin reuptake inhibitor (SSRI)/antidepressant metabolism and the subsequent response and tolerability in youth, has received limited attention. We sought to (1) review clinically relevant pharmacokinetic (PK) and pharmacodynamic (PD) interactions between cannabinoids and selected SSRIs, (2) use PK models to examine the impact of cannabinoids on SSRI exposure (area under curve (AUC)) and maximum concentration (CMAX) in adolescents, and (3) examine the frequency of adverse events reported when SSRIs and cannabinoids are used concomitantly. Cannabinoid metabolism, interactions with SSRIs, impact on relevant PK/PD pathways and known drug–drug interactions were reviewed. Then, the impact of tetrahydrocannabinol (THC) and cannabidiol (CBD) on exposure (AUC24) and CMAX for escitalopram and sertraline was modeled using pediatric PK data. Using data from the Food and Drug Administration Adverse Events Reporting System (FAERS), the relationship between CBD and CYP2C19-metabolized SSRIs and side effects was examined. Cannabis and CBD inhibit cytochrome activity, alter serotonergic transmission, and modulate SSRI response. In PK models, CBD and/or THC increases sertraline and es/citalopram concentrations in adolescents, and coadministration of CBD and CYP2C19-metabolized SSRIs increases the risk of cough, diarrhea, dizziness, and fatigue. Given the significant SSRI–cannabinoid interactions, clinicians should discuss THC and CBD use in youth prescribed SSRIs and be aware of the impact of initiating, stopping, or decreasing cannabinoid use as this may significantly affect es/citalopram and sertraline exposure.
The Effect of Dual Language Activation on L2-Induced Changes in L1 Speech within a Code-Switched Paradigm
Authors: Reubold Ulrich , Ditewig Sanne , Mayr Robert , Mennen Ineke
Published: June 2021

The present study sought to examine the effect of dual language activation on L1 speech in late English–Austrian German sequential bilinguals, and to identify relevant predictor variables. To this end, we compared the English speech patterns of adult migrants to Austria in a code-switched and monolingual condition alongside those of monolingual native speakers in England in a monolingual condition. In the code-switched materials, German words containing target segments known to trigger cross-linguistic interaction in the two languages (i.e., [v–w], [ʃt(ʁ)-st(ɹ)] and [l-ɫ]) were inserted into an English frame; monolingual materials comprised English words with the same segments. To examine whether the position of the German item affects L1 speech, the segments occurred either before the switch (“He wants a Wienerschnitzel”) or after (“I like Würstel with mustard”). Critical acoustic measures of these segments revealed no differences between the groups in the monolingual condition, but significant L2-induced shifts in the bilinguals’ L1 speech production in the code-switched condition for some sounds. These were found to occur both before and after a code-switch, and exhibited a fair amount of individual variation. Only the amount of L2 use was found to be a significant predictor variable for shift size in code-switched compared with monolingual utterances, and only for [w]. These results have important implications for the role of dual activation in the speech of late sequential bilinguals.
Differences in Hemodynamic, Hormonal and Heart Rate Variability Parameters in Complication-Free Pregnancies Compared to Individuals with Gestational Diabetes Mellitus and Preeclampsia: An Observational Retrospective Analysis
Authors: Eckstein Max L. , Moser Othmar , Rössler Andreas , Moertl Manfred G. , Jantscher Andreas , Papousek Ilona , Wagner Johann , Schmid-Zalaudek Karin , Sourij Harald , Treiber Gerlies , Lackner Helmut K.
Published: June 2021

To investigate differences in hemodynamic, hormonal and heart rate variability parameters in women following complication-free pregnancies (healthy), preeclampsia and gestational diabetes mellitus (GDM) after giving childbirth. Data of 60 women (healthy: n = 29, age 32.7 ± 4.5 years, BMI 24.2 ± 4.3 kg/m2; preeclampsia: n = 16, age 35.3 ± 4.4 years, 28.5 ± 6.4 kg/m2; GDM, n = 15, age 32.3 ± 6.0 years, BMI 26.4 ± 6.2 kg/m2) were included. Two visits were conducted 16 and 48 weeks after giving childbirth. Hair samples were taken for analysis of cortisol and testosterone. ECG and blood pressure were recorded at each visit. Data were analyzed via RM-ANOVA and post-hoc testing (p ≤ 0.05). Heart rate increased from visit 1 to visit 2, whereas SDNN decreased (both p = 0.03). RMSSD showed an increased trend for groups (p = 0.06). Testosterone in the GDM group was significantly higher compared to the other groups (p = 0.002). Cortisol levels were significantly higher following post-hoc testing GDM was different compared to healthy individuals (p = 0.02). Hemodynamic changes from week 16 to week 48 did not differ between groups (p > 0.05). No differences between individuals with preeclampsia and healthy individuals were found for all hemodynamic parameters (p > 0.05). The study showed higher levels of chronic stress indicators in GDM measured via heart rate variability and cortisol compared to women with a history of preeclampsia and healthy women.
Induction of Glutathione Synthesis Provides Cardioprotection Regulating NO, AMPK and PPARa Signaling in Ischemic Rat Hearts
Authors: Goshovska Yulia V. , Fedichkina Raisa A. , Balatskyi Volodymyr V. , Piven Oksana O. , Dobrzyn Pawel , Sagach Vadym F.
Published: June 2021

Glutathione (GSH) is essential for antioxidant defence, and its depletion is associated with tissue damage during cardiac ischemia-reperfusion (I/R). GSH is synthesized by the glutamate-cysteine ligase enzyme (GCL) from L-cysteine, which alternatively might be used for hydrogen sulfide production by cystathionine-gamma-lyase (CSE). Here, we have investigated whether in vivo treatment with L-cysteine and an inhibitor of CSE,D,L-propargylglycine (PAG), can modulate cardiac glutathione and whether this treatment can influence heart resistance to I/R in a Langendorff isolated rat hearts model. Pretreatment with PAG + L-cysteine manifested in pronounced cardioprotection, as there was complete recovery of contractile function; preserved constitutive NOS activity; and limited the production of reactive oxygen and nitrogen species in the ischemized myocardium. Cardiac GSH and GSSG levels were increased by 3.5- and 2.1-fold in PAG + L-cysteine hearts and were 3.3- and 3.6-fold higher in PAG + L-cysteine + I/R compared to I/R heart. The cardioprotective effect of PAG + L-cysteine was completely abolished by an inhibitor of GCL, DL-buthionine-(S,R)-sulfoximine. Further analysis indicated diminished fatty acid β-oxidation, increased glucose consumption and anaerobic glycolysis, and promoted OXPHOS proteins and SERCA2 in PAG + L-cysteine + I/R compared to the I/R group. PAG + L-cysteine inhibited PPARα and up-regulated AMPK signalling in the heart. Thus, induction of glutathione synthesis provided cardioprotection regulating NO, AMPK and PPARa signaling in ischemic rat hearts.
Paediatric Idiopathic Intracranial Hypertension (IIH)—A Review
Authors: Malem Andrew , Sheth Twishaa , Muthusamy Brinda
Published: June 2021

Paediatric idiopathic intracranial hypertension (IIH), is a rare but important differential diagnosis in children presenting with papilloedema. It is characterised by raised intracranial pressure in the absence of an identifiable secondary structural or systemic cause and is, therefore, a diagnosis of exclusion. In the adult population, there is a strong predilection for the disease to occur in female patients who are obese. This association is also seen in paediatric patients with IIH but primarily in the post-pubertal cohort. In younger pre-pubertal children, this is not the case, possibly reflecting a different underlying disease aetiology and pathogenesis. Untreated IIH in children can cause significant morbidity from sight loss, chronic headaches, and the psychological effects of ongoing regular hospital monitoring, interventions, and medication. The ultimate goal in the management of paediatric IIH is to protect the optic nerve from papilloedema-induced optic neuropathy and thus preserve vision, whilst reducing the morbidity from other symptoms of IIH, in particular chronic headaches. In this review, we will outline the typical work-up and diagnostic process for paediatric patients with suspected IIH and how we manage these patients.
mtDNA Heteroplasmy: Origin, Detection, Significance, and Evolutionary Consequences
Authors: Parakatselaki Maria-Eleni , Ladoukakis Emmanuel D.
Published: June 2021

Mitochondrial DNA (mtDNA) is predominately uniparentally transmitted. This results in organisms with a single type of mtDNA (homoplasmy), but two or more mtDNA haplotypes have been observed in low frequency in several species (heteroplasmy). In this review, we aim to highlight several aspects of heteroplasmy regarding its origin and its significance on mtDNA function and evolution, which has been progressively recognized in the last several years. Heteroplasmic organisms commonly occur through somatic mutations during an individual’s lifetime. They also occur due to leakage of paternal mtDNA, which rarely happens during fertilization. Alternatively, heteroplasmy can be potentially inherited maternally if an egg is already heteroplasmic. Recent advances in sequencing techniques have increased the ability to detect and quantify heteroplasmy and have revealed that mitochondrial DNA copies in the nucleus (NUMTs) can imitate true heteroplasmy. Heteroplasmy can have significant evolutionary consequences on the survival of mtDNA from the accumulation of deleterious mutations and for its coevolution with the nuclear genome. Particularly in humans, heteroplasmy plays an important role in the emergence of mitochondrial diseases and determines the success of the mitochondrial replacement therapy, a recent method that has been developed to cure mitochondrial diseases.
Interactions between Apolipoprotein E Metabolism and Retinal Inflammation in Age-Related Macular Degeneration
Authors: Hu Monica L. , Quinn Joel , Xue Kanmin
Published: June 2021

Age-related macular degeneration (AMD) is a multifactorial retinal disorder that is a major global cause of severe visual impairment. The development of an effective therapy to treat geographic atrophy, the predominant form of AMD, remains elusive due to the incomplete understanding of its pathogenesis. Central to AMD diagnosis and pathology are the hallmark lipid and proteinaceous deposits, drusen and reticular pseudodrusen, that accumulate in the subretinal pigment epithelium and subretinal spaces, respectively. Age-related changes and environmental stressors, such as smoking and a high-fat diet, are believed to interact with the many genetic risk variants that have been identified in several major biochemical pathways, including lipoprotein metabolism and the complement system. The APOE gene, encoding apolipoprotein E (APOE), is a major genetic risk factor for AMD, with the APOE2 allele conferring increased risk and APOE4 conferring reduced risk, in comparison to the wildtype APOE3. Paradoxically, APOE4 is the main genetic risk factor in Alzheimer’s disease, a disease with features of neuroinflammation and amyloid-beta deposition in common with AMD. The potential interactions of APOE with the complement system and amyloid-beta are discussed here to shed light on their roles in AMD pathogenesis, including in drusen biogenesis, immune cell activation and recruitment, and retinal inflammation.
On the Operation ∆ over Intuitionistic Fuzzy Sets
Authors: Atanassova Lilija , Dworniczak Piotr
Published: June 2021

Recently, the new operation ∆ was introduced over intuitionistic fuzzy sets and some of its properties were studied. Here, new additional properties of this operations are formulated and checked, providing an analogue to the De Morgan’s Law (Theorem 1), an analogue of the Fixed Point Theorem (Theorem 2), the connections between the operation ∆ on one hand and the classical modal operators over IFS Necessity and Possibility, on the other (Theorems 3 and 4). It is shown that it can be used for a de-i-fuzzification. A geometrical interpretation of the process of constructing the operator ∆ is given.
An Intrinsic Value Approach to Valuation with Forward–Backward Loops in Dividend Paying Stocks
Authors: Nyegaard Anna Kamille , Ott Johan Raunkjær , Steffensen Mogens
Published: June 2021

We formulate a claim valuation problem where the dynamics of the underlying asset process contain the claim value itself. The problem is motivated here by an equity valuation of a firm, with intermediary dividend payments that depend on both the underlying, that is, the assets of the company, and the equity value itself. Since the assets are reduced by the dividend payments, the entanglement of claim, claim value, and underlying is complete and numerically challenging because it forms a forward–backward stochastic system. We propose a numerical approach based on disentanglement of the forward–backward deterministic system for the intrinsic values, a parametric assumption of the claim value in its intrinsic value, and a simulation of the stochastic elements. We illustrate the method in a numerical example where the equity value is approximated efficiently, at least for the relevant ranges of the asset value.
Chirality in Geometric Algebra
Authors: Petitjean Michel
Published: June 2021

We define chirality in the context of chiral algebra. We show that it coincides with the more general chirality definition that appears in the literature, which does not require the existence of a quadratic space. Neither matrix representation of the orthogonal group nor complex numbers are used.
Iterative Methods for the Computation of the Perron Vector of Adjacency Matrices
Authors: Concas Anna , Reichel Lothar , Rodriguez Giuseppe , Zhang Yunzi
Published: June 2021

The power method is commonly applied to compute the Perron vector of large adjacency matrices. Blondel et al. [SIAM Rev. 46, 2004] investigated its performance when the adjacency matrix has multiple eigenvalues of the same magnitude. It is well known that the Lanczos method typically requires fewer iterations than the power method to determine eigenvectors with the desired accuracy. However, the Lanczos method demands more computer storage, which may make it impractical to apply to very large problems. The present paper adapts the analysis by Blondel et al. to the Lanczos and restarted Lanczos methods. The restarted methods are found to yield fast convergence and to require less computer storage than the Lanczos method. Computed examples illustrate the theory presented. Applications of the Arnoldi method are also discussed.
Eliminating Stick-Slip Vibrations in Drill-Strings with a Dual-Loop Control Strategy Optimised by the CRO-SL Algorithm
Authors: Pérez-Aracil Jorge , Camacho-Gómez Carlos , Pereira Emiliano , Vaziri Vahid , Aphale Sumeet S. , Salcedo-Sanz Sancho
Published: June 2021

Friction-induced stick-slip vibrations are one of the major causes for down-hole drill-string failures. Consequently, several nonlinear models and control approaches have been proposed to solve this problem. This work proposes a dual-loop control strategy. The inner loop damps the vibration of the system, eliminating the limit cycle due to nonlinear friction. The outer loop achieves the desired velocity with a fast time response. The optimal tuning of the control parameters is carried out with a multi-method ensemble meta-heuristic, the Coral Reefs Optimisation algorithm with Substrate Layer (CRO-SL). It is an evolutionary-type algorithm that combines different search strategies within a single population, obtaining a robust, high-performance algorithm to tackle hard optimisation problems. An application example based on a real nonlinear dynamics model of a drill-string illustrates that the controller optimised by the CRO-SL achieves excellent performance in terms of stick-slip vibrations cancellation, fast time response, robustness to system parameter uncertainties and chattering phenomenon prevention.
Relocation Scheduling in a Two-Machine Flow Shop with Resource Recycling Operations
Authors: Lo Ting-Chun , Lin Bertrand M. T.
Published: June 2021

This paper considers a variant of the relocation problem, which is formulated from an urban renewal project. There is a set of jobs to be processed in a two-machine flow shop subject to a given initial resource level. Each job consumes some units of the resource to start its processing on machine 1 and will return some amount of the resource when it is completed on machine 2. The amount of resource released by a job is not necessarily equal to the amount of resource acquired by the job for starting the process. Subject to the resource constraint, the problem is to find a feasible schedule whose makespan is minimum. In this paper, we first prove the NP-hardness of two special cases. Two heuristic algorithms with different processing characteristics, permutation and non-permutation, are designed to construct feasible schedules. Ant colony optimization (ACO) algorithms are also proposed to produce approximate solutions. We design and conduct computational experiments to appraise the performances of the proposed algorithms.
Weak Solutions and Their Kinetic Energy Regarding Time-Periodic Navier–Stokes Equations in Three Dimensional Whole-Space
Authors: Kyed Mads
Published: June 2021

The existence of weak time-periodic solutions to Navier–Stokes equations in three dimensional whole-space with time-periodic forcing terms are established. The solutions are constructed in such a way that the structural properties of their kinetic energy are obtained. No restrictions on either the size or structure of the external force are required.
How the Theme of ‘Doing and Undoing’ Applied to the Action of Exchange Reveals Overlooked Core Ideas in School Mathematics
Authors: Mason John
Published: June 2021

The theme of ‘undoing a doing’ is applied to the ubiquitous action of exchange, showing how exchange pervades a school’s mathematics curriculum. It is possible that many obstacles encountered in school mathematics arise from an impoverished sense of exchange, for learners and possibly for teachers. The approach is phenomenological, in that the reader is urged to undertake the tasks themselves, so that the pedagogical and mathematical comments, and elaborations, may connect directly to immediate experience.
Racial Differences in Incident Genitourinary Cancer Cases Captured in the National Cancer Database
Authors: Wolff Dylan T. , Monaghan Thomas F. , Gordon Danielle J. , Michelson Kyle P. , Jones Tashzna , Khargi Raymond , Smith Matthew T. , Maffucci Fenizia , Kwun Hyezo , Suss Nicholas R. , Winer Andrew G.
Published: June 2021

Background and Objectives: The National Cancer Database (NCDB) captures nearly 70% of all new cancer diagnoses in the United States, but there exists significant variation in this capture rate based on primary tumor location and other patient demographic factors. Prostate cancer has the lowest coverage rate of all major cancers, and other genitourinary malignancies likewise fall below the average NCDB case coverage rate. We aimed to explore NCDB coverage rates for patients with genitourinary cancers as a function of race. Materials and Methods: We compared the incidence of cancer cases in the NCDB with contemporary United States Cancer Statistics data. Results: Across all malignancies, American Indian/Alaskan Natives subjects demonstrated the lowest capture rates, and Asian/Pacific Islander subjects exhibited the second-lowest capture rates. Between White and Black subjects, capture rates were significantly higher for White subjects overall and for prostate cancer and kidney cancer in White males, but significantly higher for bladder cancer in Black versus White females. No significant differences were observed in coverage rates for kidney cancer in females, bladder cancer in males, penile cancer, or testicular cancer in White versus Black patients. Conclusions: Differential access to Commission on Cancer-accredited treatment facilities for racial minorities with genitourinary cancer constitutes a unique avenue for health equity research.
Heat Treatment Evaluation for the Camshafts Production of ADI Low Alloyed with Vanadium
Authors: Colin García Eduardo , Cruz Ramírez Alejandro , Reyes Castellanos Guillermo , Chávez Alcalá José Federico , Téllez Ramírez Jaime , Magaña Hernández Antonio
Published: June 2021

Ductile iron camshafts low alloyed with 0.2 and 0.3 wt % vanadium were produced by one of the largest manufacturers of the ductile iron camshafts in México “ARBOMEX S.A de C.V” by a phenolic urethane no-bake sand mold casting method. During functioning, camshafts are subject to bending and torsional stresses, and the lobe surfaces are highly loaded. Thus, high toughness and wear resistance are essential for this component. In this work, two austempering ductile iron heat treatments were evaluated to increase the mechanical properties of tensile strength, hardness, and toughness of the ductile iron camshaft low alloyed with vanadium. The austempering process was held at 265 and 305 °C and austempering times of 30, 60, 90, and 120 min. The volume fraction of high-carbon austenite was determined for the heat treatment conditions by XRD measurements. The ausferritic matrix was determined in 90 min for both austempering temperatures, having a good agreement with the microstructural and hardness evolution as the austempering time increased. The mechanical properties of tensile strength, hardness, and toughness were evaluated from samples obtained from the camshaft and the standard Keel block. The highest mechanical properties were obtained for the austempering heat treatment of 265 °C for 90 min for the ADI containing 0.3 wt % V. The tensile and yield strength were 1200 and 1051 MPa, respectively, while the hardness and the energy impact values were of 47 HRC and 26 J; these values are in the range expected for an ADI grade 3.
Hydrogen Stress Cracking Behaviour in Dissimilar Welded Joints of Duplex Stainless Steel and Carbon Steel
Authors: Park Hanji , Moon Byungrok , Moon Younghoon , Kang Namhyun
Published: June 2021

As the need for duplex stainless steel (DSS) increases, it is necessary to evaluate hydrogen stress cracking (HSC) in dissimilar welded joints (WJs) of DSS and carbon steel. This study aims to investigate the effect of the weld microstructure on the HSC behaviour of dissimilar gas-tungsten arc welds of DSS and carbon steel. In situ slow-strain rate testing (SSRT) with hydrogen charging was conducted for transverse WJs, which fractured in the softened heat-affected zone of the carbon steel under hydrogen-free conditions. However, HSC occurred at the martensite band and the interface of the austenite and martensite bands in the type-II boundary. The band acted as an HSC initiation site because of the presence of a large amount of trapped hydrogen and a high strain concentration during the SSRT with hydrogen charging. Even though some weld microstructures such as the austenite and martensite bands in type-II boundaries were harmless under normal hydrogen-free conditions, they had a negative effect in a hydrogen atmosphere, resulting in the premature rupture of the weld. Eventually, a premature fracture occurred during the in situ SSRT in the type-II boundary because of the hydrogen-enhanced strain-induced void (HESIV) and hydrogen-enhanced localised plasticity (HELP) mechanisms.
Sub-Microstructure of Surface and Subsurface Layers after Electrical Discharge Machining Structural Materials in Water
Authors: Grigoriev Sergey N. , Volosova Marina A. , Okunkova Anna A. , Fedorov Sergey V. , Hamdy Khaled , Podrabinnik Pavel A.
Published: June 2021

The material removal mechanism, submicrostructure of surface and subsurface layers, nanotransformations occurred in surface and subsurface layers during electrical discharge machining two structural materials such as anti-corrosion X10CrNiTi18-10 (12kH18N10T) steel of austenite class and 2024 (D16) duralumin in a deionized water medium were researched. The machining was conducted using a brass tool of 0.25 mm in diameter. The measured discharge gap is 45–60 µm for X10CrNiTi18-10 (12kH18N10T) steel and 105–120 µm for 2024 (D16) duralumin. Surface roughness parameters are arithmetic mean deviation (Ra) of 4.61 µm, 10-point height (Rz) of 28.73 µm, maximum peak-to-valley height (Rtm) of 29.50 µm, mean spacing between peaks (Sm) of 18.0 µm for steel; Ra of 5.41 µm, Rz of 35.29 µm, Rtm of 43.17 µm, Sm of 30.0 µm for duralumin. The recast layer with adsorbed components of the wire tool electrode and carbides was observed up to the depth of 4–6 µm for steel and 2.5–4 µm for duralumin. The Levenberg–Marquardt algorithm was used to mathematically interpolate the dependence of the interelectrode gap on the electrical resistance of the material. The observed microstructures provide grounding on the nature of electrical wear and nanomodification of the obtained surfaces.
Hybrid Organic-Inorganic Materials on Metallic Surfaces: Fabrication and Electrochemical Performance
Authors: Nashrah Nisa , Putri Rosy Amalia Kurnia , Alharissa Early Zahwa , Al Zoubi Wail , Ko Young Gun
Published: June 2021

In recent years, hybrid organic-inorganic (HOI) materials have attracted massive attention as they combine the unique properties of organic and inorganic compounds. In this review, we focus on the formation of HOI materials and their electrochemical performance that can be controlled by microstructural design depending upon their chemical composition. This overview outlines the recent strategies of preparing HOI materials on metallic surface via wet-electrochemical systems, such as plasma electrolysis (PE) and dip chemical coating (DCC). The corresponding electrochemical behavior for short and long term exposures is also summarized.
Engraftment of Bacteria after Fecal Microbiota Transplantation Is Dependent on Both Frequency of Dosing and Duration of Preparative Antibiotic Regimen
Authors: Gopalakrishnan Vancheswaran , Dozier Elizabeth Ashley , Glover Matthew S. , Novick Steven , Ford Michael , Morehouse Christopher , Warrener Paul , Caceres Carolina , Hess Sonja , Sellman Bret R. , Cohen Taylor S.
Published: June 2021

The gut microbiota has emerged as a key mediator of human physiology, and germ-free mice have been essential in demonstrating a role for the microbiome in disease. Preclinical models using conventional mice offer the advantage of working with a mature immune system. However, optimal protocols for fecal microbiota transplant (FMT) engraftment in conventional mice are yet to be established. Conventional BALB/c mice were randomized to receive 3-day (3d) or 3-week (3w) antibiotic (ABX) regimen in their drinking water followed by 1 or 5-daily FMTs from a human donor. Fecal samples were collected longitudinally and characterized using 16S ribosomal RNA (rRNA) sequencing. Semi-targeted metabolomic profiling of fecal samples was also done with liquid chromatography–mass spectrometry (LC-MS). Lastly, we sought to confirm our findings in BKS mice. Recovery of baseline diversity scores were greatest in the 3d groups, driven by re-emergence of mouse commensal microbiota, whereas the most resemblance to donor microbiota was seen in the 3w + 5-FMT group. Amplicon sequence variants (ASVs) that were linked to the input material (human ASVs) engrafted to a significantly greater extent when compared to mouse ASVs in the 3-week groups but not the 3-day groups. Lastly, comparison of metabolomic profiles revealed distinct functional profiles by ABX regimen. These results indicate successful model optimization and emphasize the importance of ABX duration and frequency of FMT dosing; the most stable and reliable colonization by donor ASVs was seen in the 3wk + 5-FMT group.
Exosomal microRNA let-7-5p from Taenia pisiformis Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ
Authors: Wang Liqun , Liu Tingli , Chen Guoliang , Li Yanping , Zhang Shaohua , Mao Li , Liang Panhong , Fasihi Harandi Majid , Li Taoshan , Luo Xuenong
Published: June 2021

Cysticercus pisiformis, the larval stage of Taenia pisiformis, causes serious illness in rabbits that severely impacts the rabbit breeding industry. An inhibitive Th2 immune response can be induced by let-7-enriched exosomes derived from T. pisiformis cysticercus. However, the underlying molecular mechanisms are not completely understood. Here, we report that exosomal miR-let-7-5p released by T. pisiformis cysticercus played a critical role in the activation of M2 macrophages. We found that overexpression of let-7-5p in M1 macrophages decreased M1 phenotype expression while promoting polarization to the M2 phenotype, which is consistent with experimental data in exosome-treated macrophages alone. In contrast, knockdown of let-7-5p in exosome-like vesicles promoted M1 polarization and decreased M2 phenotype expression. Furthermore, down-regulation of transcription factor CCAAT/enhancer-binding protein (C/EBP)-δ resulted in the decrease of M1 phenotype markers and increase of M2 phenotype markers. These results suggested that let-7 enriched in exosome-like vesicles from T. pisiformis metacestodes can induce M2 macrophage polarization via targeting C/EBP δ, which may be involved in macrophage polarization induced by T. pisiformis metacestodes. The finding helps to expand our knowledge of the molecular mechanism of immunosuppression and Th2 immune response induced by metacestodes.
Effects of the Denitrification Inhibitor “Procyanidins” on the Diversity, Interactions, and Potential Functions of Rhizosphere-Associated Microbiome
Authors: Hugoni Mylène , Galland William , Lecomte Solène , Bruto Maxime , Barakat Mohamed , Piola Florence , Achouak Wafa , Haichar Feth el Zahar
Published: June 2021

Some plant secondary metabolites, such as procyanidins, have been demonstrated to cause biological denitrification inhibition (BDI) of denitrifiers in soils concomitantly with a gain in plant biomass. The present work evaluated whether procyanidins had an impact on the diversity of nontarget microbial communities that are probably involved in soil fertility and ecosystem services. Lettuce plants were grown in two contrasting soils, namely Manziat (a loamy sand soil) and Serail (a sandy clay loam soil) with and without procyanidin amendment. Microbial diversity was assessed using Illumina sequencing of prokaryotic 16S rRNA gene and fungal ITS regions. We used a functional inference to evaluate the putative microbial functions present in both soils and reconstructed the microbial interaction network. The results showed a segregation of soil microbiomes present in Serail and Manziat that were dependent on specific soil edaphic variables. For example, Deltaproteobacteria was related to total nitrogen content in Manziat, while Leotiomycetes and Firmicutes were linked to Ca2+ in Serail. Procyanidin amendment did not affect the diversity and putative activity of microbial communities. In contrast, microbial interactions differed according to procyanidin amendment, with the results showing an enrichment of Entotheonellaeota and Mucoromycota in Serail soil and of Dependentiae and Rozellomycetes in Manziat soil.
Pitfalls and Possibilities of Patinated Bronze: The Analysis of Pre-Roman Italian Armour Using pXRF
Authors: Emmitt Joshua , McAlister Andrew , Armstrong Jeremy
Published: June 2021

Despite the importance of weapons and armour as part of material culture in the Mediterranean during the first millennium BCE, such objects have generally not been studied beyond stylistic analyses. Bronze was extensively used in the construction of these materials; however, its characterisation is complicated due to patination and the different manufacturing techniques used. We used portable XRF (pXRF) to non-destructively characterise bronze material in a way that mitigates the distorting effects of patina. Analysis was conducted on 23 pieces of pre-Roman Italian bronze armour. Assays were taken using two different techniques; ‘single point’ assays and ‘cluster’ assays. There is variability visible across assays both on and between items, grouped both geographically and chronologically. We highlight significant trends visible in the results over time and different object types and discuss the utility of pXRF on ancient bronze with recommendations for best practice.
Use of Propionibacterium freudenreichii T82 Strain for Effective Biosynthesis of Propionic Acid and Trehalose in a Medium with Apple Pomace Extract and Potato Wastewater
Authors: Piwowarek Kamil , Lipińska Edyta , Hać-Szymańczuk Elżbieta , Kot Anna Maria , Kieliszek Marek , Bonin Sylwia
Published: June 2021

Propionic acid bacteria are the source of many metabolites, e.g., propionic acid and trehalose. Compared to microbiological synthesis, the production of these metabolites by petrochemical means or enzymatic conversion is more profitable. The components of microbiological media account for a large part of the costs associated with propionic fermentation, due to the high nutritional requirements of Propionibacterium. This problem can be overcome by formulating a medium based on the by-products of technological processes, which can act as nutritional sources and at the same time replace expensive laboratory preparations (e.g., peptone and yeast extract). The metabolic activity of P. freudenreichii was investigated in two different breeding environments: in a medium containing peptone, yeast extract, and biotin, and in a waste-based medium consisting of only apple pomace and potato wastewater. The highest production of propionic acid amounting to 14.54 g/L was obtained in the medium containing apple pomace and pure laboratory supplements with a yield of 0.44 g/g. Importantly, the acid production parameters in the waste medium reached almost the same level (12.71 g/L, 0.42 g/g) as the medium containing pure supplements. Acetic acid synthesis was more efficient in the waste medium; it was also characterized by a higher level of accumulated trehalose (59.8 mg/g d.s.). Thus, the obtained results show that P. freudenreichii bacteria exhibited relatively high metabolic activity in an environment with apple pomace used as a carbon source and potato wastewater used as a nitrogen source. This method of propioniate production could be cheaper and more sustainable than the chemical manner.
A Computational Protocol Combining DFT and Cheminformatics for Prediction of pH-Dependent Redox Potentials
Authors: Fornari Rocco Peter , de Silva Piotr
Published: June 2021

Discovering new materials for energy storage requires reliable and efficient protocols for predicting key properties of unknown compounds. In the context of the search for new organic electrolytes for redox flow batteries, we present and validate a robust procedure to calculate the redox potentials of organic molecules at any pH value, using widely available quantum chemistry and cheminformatics methods. Using a consistent experimental data set for validation, we explore and compare a few different methods for calculating reaction free energies, the treatment of solvation, and the effect of pH on redox potentials. We find that the B3LYP hybrid functional with the COSMO solvation method, in conjunction with thermal contributions evaluated from BLYP gas-phase harmonic frequencies, yields a good prediction of pH = 0 redox potentials at a moderate computational cost. To predict how the potentials are affected by pH, we propose an improved version of the Alberty-Legendre transform that allows the construction of a more realistic Pourbaix diagram by taking into account how the protonation state changes with pH.
Effects of Graphene Oxidation on Interaction Energy and Interfacial Thermal Conductivity of Polymer Nanocomposite: A Molecular Dynamics Approach
Authors: Bellussi Francesco Maria , Sáenz Ezquerro Carlos , Laspalas Manuel , Chiminelli Agustín
Published: June 2021

Interfacial characteristics of polymer nanocomposites represent a crucial aspect to understand their global properties and to evaluate the interaction between nanofillers and matrix. In this work we used a molecular dynamics (MD) approach to characterize the interfacial region at the atomistic scale of graphene-based polymer nanocomposites. Three different polymer matrixes were considered, polylactic acid (PLA), polypropylene (PP) and epoxy resin (EPO), which were reinforced with three types of graphene fillers: pristine graphene (G), graphene oxide (GO) and reduced graphene oxide (rGO). In particular, the compatibility of the nanofillers in polymer matrixes were evaluated in terms of the interaction energy, while the interfacial thermal resistance (Kapitza resistance) between matrices and fillers was calculated with a nonequilibrium molecular dynamics (NEMD) method. Results showed that the oxidation degree plays an important role on the studied properties of the interfacial region. In particular, it was observed that the Kapitza resistance is decreased in the oxidized graphene (GO and rGO), while interaction energy depended on the polarity of the polymer matrix molecules and the contribution of the Coulombic component.
An Overview of Functionalized Graphene Nanomaterials for Advanced Applications
Authors: Maio Andrea , Pibiri Ivana , Morreale Marco , Mantia Francesco , Scaffaro Roberto
Published: June 2021

Interest in the development of graphene-based materials for advanced applications is growing, because of the unique features of such nanomaterials and, above all, of their outstanding versatility, which enables several functionalization pathways that lead to materials with extremely tunable properties and architectures. This review is focused on the careful examination of relationships between synthetic approaches currently used to derivatize graphene, main properties achieved, and target applications proposed. Use of functionalized graphene nanomaterials in six engineering areas (materials with enhanced mechanical and thermal performance, energy, sensors, biomedical, water treatment, and catalysis) was critically reviewed, pointing out the latest advances and potential challenges associated with the application of such materials, with a major focus on the effect that the physicochemical features imparted by functionalization routes exert on the achievement of ultimate properties capable of satisfying or even improving the current demand in each field. Finally, current limitations in terms of basic scientific knowledge and nanotechnology were highlighted, along with the potential future directions towards the full exploitation of such fascinating nanomaterials.
Metasurface of Combined Semicircular Rings with Orthogonal Slit Pairs for Generation of Dual Vector Beams
Authors: Kong Qian , Gu Manna , Zeng Xiangyu , Sun Rui , Zhang Yuqin , Liu Chunxiang , Ma Hong , Gui Weiling , Cheng Chuanfu
Published: June 2021

Manipulation of multichannel vector beams (VBs) with metasurfaces is an important topic and holds potential applications in information technology. In this paper, we propose a novel metasurface for the generation of dual VBs, which is composed of orthogonal slit pairs arranged on multiple groups of combined semicircular rings (CSRs). A group of CSRs include a right-shifted set and a left-shifted set of semicircular rings, and each set of semicircular rings has two halves of circles with different radii, sharing the same shifted center. Under the illumination of linearly polarized light, the two shifted sets of semicircular rings generate the two VBs at the shifted center positions on the observation plane. The slit units of each set are designed with independent rotation order and initial orientation angle. By adjusting the linear polarization of illumination, both two VBs with their orders and polarization states are independently controlled simultaneously. The principle and design are demonstrated by the finite-difference time domain (FDTD) simulation. The work is of significance for miniatured devices of VB generators and for related applications.
The Role of Micronutrients in Ageing Asia: What Can Be Implemented with the Existing Insights
Authors: Inui Taichi , Hanley Bryan , Tee E Siong , Nishihira Jun , Tontisirin Kraisid , Van Dael Peter , Eggersdorfer Manfred
Published: June 2021

Life expectancy as a measure of population health does not reflect years of healthy life. The average life expectancy in the Asia-Pacific region has more than doubled since 1900 and is now above 70 years. In the Asia-Pacific region, the proportion of aged people in the population is expected to double between 2017 and 2050. Increased life expectancy leads to an increase in non-communicable diseases, which consequently affects quality of life. Suboptimal nutritional status is a contributing factor to the prevalence and severity of non-communicable diseases, including cardiovascular, cognitive, musculoskeletal, immune, metabolic and ophthalmological functions. We have reviewed the published literature on nutrition and healthy ageing as it applies to the Asia-Pacific region, focusing on vitamins, minerals/trace elements and omega-3 fatty acids. Optimal nutritional status needs to start before a senior age is reached and before the consequences of the disease process are irreversible. Based on the nutritional status and health issues in the senior age in the region, micronutrients of particular importance are vitamins A, D, E, C, B-12, zinc and omega-3 fatty acids. The present paper substantiates the creation of micronutrient guidelines and proposes actions to support the achievement of optimal nutritional status as contribution to healthy ageing for Asia-Pacific populations.
Impacts of Infectious Dose, Feeding Behavior, and Age of Culicoides sonorensis Biting Midges on Infection Dynamics of Vesicular Stomatitis Virus
Authors: Rozo-Lopez Paula , Londono-Renteria Berlin , Drolet Barbara S.
Published: June 2021

Culicoides sonorensis biting midges are biological vectors of vesicular stomatitis virus (VSV) in the U.S. Yet, little is known regarding the amount of ingested virus required to infect midges, nor how their feeding behavior or age affects viral replication and vector competence. We determined the minimum infectious dose of VSV-New Jersey for C. sonorensis midges and examined the effects of multiple blood-feeding cycles and age at the time of virus acquisition on infection dynamics. A minimum dose of 3.2 logs of virus/mL of blood resulted in midgut infections, and 5.2 logs/mL resulted in a disseminated infection to salivary glands. For blood-feeding behavior studies, ingestion of one or two non-infectious blood meals (BM) after a VSV infectious blood meal (VSV-BM) resulted in higher whole-body virus titers than midges receiving only the single infectious VSV-BM. Interestingly, this infection enhancement was not seen when a non-infectious BM preceded the infectious VSV-BM. Lastly, increased midge age at the time of infection correlated to increased whole-body virus titers. This research highlights the epidemiological implications of infectious doses, vector feeding behaviors, and vector age on VSV infection dynamics to estimate the risk of transmission by Culicoides midges more precisely.
How Bacteria Change after Exposure to Silver Nanoformulations: Analysis of the Genome and Outer Membrane Proteome
Authors: Kędziora Anna , Speruda Mateusz , Wernecki Maciej , Dudek Bartłomiej , Kapczynska Katarzyna , Krzyżewska Eva , Rybka Jacek , Bugla-Płoskońska Gabriela
Published: June 2021

Objective: the main purpose of this work was to compare the genetic and phenotypic changes of E. coli treated with silver nanoformulations (E. coli BW25113 wt, E. coli BW25113 AgR, E. coli J53, E. coli ATCC 11229 wt, E. coli ATCC 11229 var. S2 and E. coli ATCC 11229 var. S7). Silver, as the metal with promising antibacterial properties, is currently widely used in medicine and the biomedical industry, in both ionic and nanoparticles forms. Silver nanoformulations are usually considered as one type of antibacterial agent, but their physical and chemical properties determine the way of interactions with the bacterial cell, the mode of action, and the bacterial cell response to silver. Methods: the changes in the bacterial genome, resulting from the treatment of bacteria with various silver nanoformulations, were verified by analyzing of genes (selected with mutfunc) and their conservative and non-conservative mutations selected with BLOSUM62. The phenotype was verified using an outer membrane proteome analysis (OMP isolation, 2-DE electrophoresis, and MS protein identification). Results: the variety of genetic and phenotypic changes in E. coli strains depends on the type of silver used for bacteria treatment. The most changes were identified in E. coli ATCC 11229 treated with silver nanoformulation signed as S2 (E. coli ATCC 11229 var. S2). We pinpointed 39 genes encoding proteins located in the outer membrane, 40 genes of their regulators, and 22 genes related to other outer membrane structures, such as flagellum, fimbria, lipopolysaccharide (LPS), or exopolysaccharide in this strain. Optical density of OmpC protein in E. coli electropherograms decreased after exposure to silver nanoformulation S7 (noticed in E. coli ATCC 11229 var. S7), and increased after treatment with the other silver nanoformulations (SNF) marked as S2 (noticed in E. coli ATCC 11229 var. S2). Increase of FliC protein optical density was identified in turn after Ag+ treatment (noticed in E.coli AgR). Conclusion: the results show that silver nanoformulations (SNF) exerts a selective pressure on bacteria causing both conservative and non-conservative mutations. The proteomic approach revealed that the levels of some proteins have changed after treatment with appropriate SNF.