bluuebunny/crossref_metadata_2025 huggingface dataset:
A randomised controlled trial to investigate the clinical effectiveness and cost effectiveness of Mindfulness-Based Cognitive Therapy (MBCT) for depressed non-responders to Increasing Access to Psychological Therapies (IAPT) high-intensity therapies: study protocol
Authors: Barnhofer Thorsten , Dunn Barnaby D. , Strauss Clara , Ruths Florian , Barrett Barbara , Ryan Mary , Ladwa Asha , Stafford Frances , Fichera Roberta , Baber Hannah , McGuinness Ailis , Metcalfe Isabella , Harding Delilah , Walker Sarah , Ganguli Poushali , Rhodes Shelley , Young Allan , Warren Fiona
Published: January 2023

Background

Major depression represents a pressing challenge for health care. In England, Increasing Access to Psychological Therapies (IAPT) services provide evidence-based psychological therapies in a stepped-care approach to patients with depression. While introduction of these services has successfully increased access to therapy, estimates suggest that about 50% of depressed patients who have come to the end of the IAPT pathway still show significant levels of symptoms. This study will investigate whether Mindfulness-Based Cognitive Therapy (MBCT), a group intervention combining training in mindfulness meditation and elements from cognitive therapy, can have beneficial effects in depressed patients who have not responded to high-intensity therapy in IAPT. It will seek to establish the effectiveness and cost-effectiveness of MBCT as compared to the treatment these patients would usually receive.

Methods

In a 2-arm randomised controlled trial, patients who currently meet the criteria for major depressive disorder and who have not sufficiently responded to at least 12 sessions of IAPT high-intensity therapy will be allocated, at a ratio of 1:1, to receive either MBCT (in addition to treatment as usual [TAU]) or continue with TAU only. Assessments will take place at baseline, 10 weeks and 34 weeks post-randomisation. The primary outcome will be reduction in depression symptomatology 34 weeks post-randomisation as assessed using the Public Health Questionnaire-9 (PHQ-9). Secondary outcomes will include depressive symptomatology at 10 weeks post-randomisation and other clinical outcomes measured at 10-week and 34-week follow-up, along with a series of binarised outcomes to indicate clinically significant and reliable change. Evaluations of cost-effectiveness will be based on assessments of service use costs collected using the Adult Service Use Schedule and health utilities derived from the EQ-5D.

Discussion

This trial will add to the evidence base for the use of MBCT in depressed treatment non-responders. It will constitute the first trial to test MBCT following non-response to psychological therapy, with results providing a direct estimate of efficacy within the IAPT pathway. As such, its results will offer an important basis for decisions regarding the adoption of MBCT for non-responders within IAPT.

Trial registration

ClinicalTrials.gov NCT05236959. Registered on 11 February 2022. ISRCTN 17755571. Registered on 2 February 2021.
Effect of high-frequency repetitive transcranial magnetic stimulation under different intensities upon rehabilitation of chronic pelvic pain syndrome: protocol for a randomized controlled trial
Authors: Wang Mengyang , Xia Rui , Shi Jiao , Yang Chunhua , Zhang Yongqing , Xu Zhengxian , Yu Cancan , Wu Ziyi , Wang Min , Chen Shangjie , Qu Hongdang
Published: January 2023

Introduction

Nearly one in seven women worldwide suffers from chronic pelvic pain syndrome (CPPS) each year. Often, CPPS necessitates a combination of treatments. Studies have shown the good therapeutic effects of repetitive transcranial magnetic stimulation (rTMS) upon CPPS. We wish to undertake a randomized controlled trial (RCT) to observe the effect of high-frequency rTMS at different intensities upon CPPS.

Methods and analyses

In this prospective, double-blinded RCT, 63 female CPPS participants will be recruited and randomized (1:1:1) to high-intensity rTMS, low-intensity rTMS, or sham rTMS. The control group will receive a 10-day course of conventional pelvic floor (PF) rehabilitation (neuromuscular stimulation, magnetic therapy, or light therapy of the PF). On the basis of conventional treatment, participants in the high-intensity rTMS group will receive pulses of 10 Hz with a resting motor threshold (RMT) of 110% for a total of 15,000 pulses. Participants in the low-intensity rTMS group will receive pulses of 10 Hz with an RMT of 80% with 15,000 pulses. The sham rTMS group will be subjected to sham stimulation with the same sound as produced by the real magnetic stimulation coil. The primary outcome will be determined using a visual analog scale, the Genitourinary Pain Index, Zung Self-Rating Anxiety Scale, and Zung Self-Rating Depression Scale. The secondary outcome will be determined by electromyography of the surface of PF muscles at baseline and after treatment completion.

Ethics and dissemination

This study is approved by the Ethics Committee of Bao’an People’s Hospital, Shenzhen, Guangdong Province (approval number: BYL20211203). The results will be submitted for publication in peer-reviewed journals and disseminated at scientific conferences (Protocol version 1.0-20220709).

Trial registration

Chictr.org.cn, ID: ChiCTR2200055615. Registered on 14 January 2022, http://www.chictr.org.cn/showproj.aspx?proj=146720. Protocol version 1.0-20220709.
The growth, lipid accumulation and adaptation mechanism in response to variation of temperature and nitrogen supply in psychrotrophic filamentous microalga Xanthonema hormidioides (Xanthophyceae)
Authors: Gao Baoyan , Hong Jian , Chen Jiamin , Zhang Hu , Hu Ren , Zhang Chengwu
Published: January 2023

Background

Microalgae are promising feedstocks for production of renewable biofuels and value-added bioproducts. Temperature and nitrogen supply are important environmental and nutritional factors affecting the growth and metabolism of microalgae, respectively. In this study, the growth and lipid accumulation of filamentous microalgae Xanthonema hormidioides under different temperatures (5, 7, 10, 15, 20, 25, 27 and 30 °C) and initial nitrogen concentrations (3, 9, 18 mM) were investigated, and its adaptive mechanisms of tolerance to low temperature and nitrogen stress were analysis by proteomics.

Results

The optimum temperature range for the growth of X. hormidioides was between 15 and 20 °C, and the algal cells had slow growth rate at 5 °C and could not survive at 30 °C. The maximum biomass concentration was 11.73 g L−1 under the temperature of 20 °C, and the highest total lipid content was 56.63% of dry weight. Low temperature did not change the fatty acids profiles but promoted the accumulation of unsaturated fatty acids of X. hormidioides. The maximum contents of palmitoleic acid, eicosapentaenoic acid and total fatty acid were 23.64%, 2.49% and 41.14% of dry weight, respectively. Proteomics was performed under three temperature (7, 15, 25 °C), two nitrogen concentrations (3 and 18 mM) and two cultivation times (day 3 and 12). A total of 6503 proteins were identified. In the low temperature, photosynthesis-related proteins were down-regulated to protect the photosynthetic apparatus. The up-regulation of key enzymes DGAT and PDAT demonstrated the accumulation of TAGs under low nitrogen treatment. The proteins related to ribosome, phosphatidylinositol signaling system, antioxidant system and cold shock proteins (CSPs) in X. hormidioides were co-upregulated under the treatment of low temperature, which can alleviate the damages induced by temperature stress and maintain the normal growth and metabolism of algal cells.

Conclusions

X. hormidioides is a psychrotolerant microalga. It is an oleaginous filamentous microalga containing hyper palmitoleic acid and a certain amount of eicosapentaenoic acid with great potential for biofuel development, as well as for applications in nutritional health products and other industries.
How can gender be identified from heart rate data? Evaluation using ALLSTAR heart rate variability big data analysis
Authors: Kaneko Itaru , Hayano Junichiro , Yuda Emi
Published: January 2023

Objective

A small electrocardiograph and Holter electrocardiograph can record an electrocardiogram for 24 h or more. We examined whether gender could be verified from such an electrocardiogram and, if possible, how accurate it would be.

Results

Ten dimensional statistics were extracted from the heart rate data of more than 420,000 people, and gender identification was performed by various major identification methods. Lasso, linear regression, SVM, random forest, logistic regression, k-means, Elastic Net were compared, for Age < 50 and Age ≥ 50. The best Accuracy was 0.681927 for Random Forest for Age < 50. There are no consistent difference between Age < 50 and Age ≥ 50. Although the discrimination results based on these statistics are statistically significant, it was confirmed that they are not accurate enough to determine the gender of an individual.
Improving the thermostability of GH49 dextranase AoDex by site-directed mutagenesis
Authors: Wei Zhen , Chen Jinling , Xu Linxiang , Liu Nannan , Yang Jie , Wang Shujun
Published: January 2023

As an indispensable enzyme for the hydrolysis of dextran, dextranase has been widely used in the fields of food and medicine. It should be noted that the weak thermostability of dextranase has become a restricted factor for industrial applications. This study aims to improve the thermostability of dextranase AoDex in glycoside hydrolase (GH) family 49 that derived from Arthrobacter oxydans KQ11. Some mutants were predicted and constructed based on B-factor analysis, PoPMuSiC and HotMuSiC algorithms, and four mutants exhibited higher heat resistance. Compared with the wild-type, mutant S357P showed the best improved thermostability with a 5.4-fold increase of half-life at 60 °C, and a 2.1-fold increase of half-life at 65 °C. Furthermore, S357V displayed the most obvious increase in enzymatic activity and thermostability simultaneously. Structural modeling analysis indicated that the improved thermostability of mutants might be attributed to the introduction of proline and hydrophobic effects, which generated the rigid optimization of the structural conformation. These results illustrated that it was effective to improve the thermostability of dextranase AoDex by rational design and site-directed mutagenesis. The thermostable mutant of dextranase AoDex has potential application value, and it can also provide references for engineering other thermostable dextranases of the GH49 family.
Trainable windows for SincNet architecture
Authors: H C Prashanth , Rao Madhav , Eledath Dhanya , V Ramasubramanian
Published: January 2023

SincNet architecture has shown significant benefits over traditional Convolutional Neural Networks (CNN), especially for speaker recognition applications. SincNet comprises parameterized Sinc functions as filters in the first layer followed by convolutional layers. Although SincNet is compact in nature and offers top-level understanding of the features extracted, the effect of window function used in SincNet is not thoroughly addressed yet. Hamming and Hann are popularly used as the default time-localized windows to reduce spectral leakage. Hence, a comprehensive investigation of 28 different windowing functions on SincNet architecture towards speaker recognition task using TIMIT dataset was performed in this work. Additionally, “trainable” window functions were configured with tunable parameters to characterize the performance. The paper benchmarks the effect of the time-localized windowing function in terms of the bandwidth, side-lobe suppression, and spectral leakage for the filter banks employed in the first layer of the SincNet architecture. Trainable Gaussian and Cosine-Sum functions exhibited relative improvement of 41.46% and 82.11% in the sentence level classification error rate over Hamming window when employed on SincNet architecture.
Identifying effect modifiers of CAR-T cell therapeutic efficacy: a systematic review and individual patient data meta-analysis protocol
Authors: Lalu Manoj M. , Kekre Natasha , Montroy Joshua , Ghiasi Maryam , Hay Kevin , McComb Scott , Weeratna Risini , Atkins Harold , Hutton Brian , Yahya Ayel , Masurekar Ashish , Sobh Mohamad , Fergusson Dean A.
Published: January 2023

Background

Chimeric antigen receptor T cell therapy (CAR-T) represents a promising and exciting new therapy for hematologic malignancies, where prognosis for relapsed/refractory patients remains poor. Encouraging results from clinical trials have often been tempered by heterogeneity in response to treatment among patients, as well as safety concerns including cytokine release syndrome. The identification of specific patient or treatment-specific factors underlying this heterogeneity may provide the key to the long-term sustainability of this complex and expensive therapy. An individual patient data meta-analysis (IPMDA) may provide potential explanations for the high degree of heterogeneity. Therefore, our objective is to perform a systematic review and IPDMA of CAR-T cell therapy in patients with hematologic malignancies to explore potential effect modifiers of CAR-T cell therapy.

Methods and analysis

We will search MEDLINE, Embase, and the Cochrane Central Register of Controlled Clinical Trials. Studies will be screened in duplicate at the abstract level, then at the full-text level by two independent reviewers. We will include any prospective clinical trial of CAR-T cell therapy in patients with hematologic malignancies. Our primary outcome is complete response, while secondary outcomes of interest include overall response, progression-free survival, overall survival, and safety. IPD will be collected from each included trial and, in the case of missing data, corresponding authors/study sponsors will be contacted. Standard aggregate meta-analyses will be performed, followed by the IPD meta-analysis using a one-stage approach. A modified Institute of Health Economics tool will be used to evaluate the risk of bias of included studies.

Ethics and dissemination

Identifying characteristics that may act as modifiers of CAR-T cell efficacy is of paramount importance and can help shape future clinical trials in the field. Results from this study will be submitted for publication in a peer-reviewed scientific journal, presented at relevant conferences and shared with relevant stakeholders.
Significant increase of serum extracellular vesicle-packaged growth differentiation factor 15 in type 2 diabetes mellitus: a cross-sectional study
Authors: Zhao Wen , Li Xinwei , Li Xinxin , Peng Lu , Li Yu , Du Yunhui , He Jianxun , Qin Yanwen , Zhang Huina
Published: January 2023

Background

Growth differentiation factor 15 (GDF15) is a stress-inducible factor involved in the inflammatory progression of many complications, including type 2 diabetes mellitus (T2DM). Growing evidence suggests that molecules in extracellular vesicles (EVs) are associated with diabetes or diabetes-related complications. However, the correlation between serum extracellular vesicle-derived growth differentiation factor15 (EV-GDF15) and T2DM is unknown. The aim of this cross-sectional study is to investigate whether serum EV-GDF15 is associated with T2DM incidence.

Methods

116 individuals, including 78 T2DM and 38 non-T2DM, were recruited as participants. The concentrations of serum EV-GDF15 and serum GDF15 were determined by Luminex assay. Serum EVs were obtained by ultracentrifugation. Multivariate stepwise regression analysis was used to determine the association between serum GDF15 levels and fasting plasma glucose (FPG) as well as glycated hemoglobin (HbA1c). The association of serum EV-GDF15 levels with T2DM was determined by multivariate logistic regression analysis.

Results

Our data showed that the levels of serum EV-GDF15 and serum GDF15 were significantly increased in T2DM patients compared with non-T2DM subjects (EV-GDF15 levels, 13.68 (6.61–23.44) pg/mL vs. 5.56 (3.44–12.09) pg/mL, P < 0.001; and serum GDF15 levels, 1025.49 (677.87–1626.36) pg/mL vs. 675.46 (469.53–919.98) pg/mL, P < 0.001). There was a linear correlation between EV-GDF15 levels and fasting plasma glucose (FPG) and Hemoglobin A1C (HbA1c) levels (normalized β = 0.357, P < 0.001; normalized β = 0.409, P < 0.001, respectively). Elevated levels of EV-GDF15 were accompanied by an increase in the proportion of patients with T2DM (from 47.5 to 78.9%) and a progressive independent association with the incidence of T2DM (from OR = 3.06, 95% CI 1.02–9.19, P = 0.047 to OR = 3.75, 95% CI 1.14–12.26, P = 0.029). Notably, high levels of serum GDF15 plus high levels of serum EV-GDF15 were significantly associated with T2DM more than either alone.

Conclusion

This study elucidated that increased levels of GDF15 in serum EVs were independently associated with T2DM.
Laboratory measurements show temperature-dependent permittivity of lunar regolith simulants
Authors: Kobayashi M. , Miyamoto H. , Pál B. D. , Niihara T. , Takemura T.
Published: January 2023

The mapping of available water–ice is a crucial step in the lunar exploration missions. Ground penetrating radars have the potential to map the subsurface structure and the existence of water–ice in terms of the electromagnetic properties, specifically, the permittivity. Slight differences in permittivity can be significantly important when applied in a dry environment, such as on the Moon and Mars. The capability of detecting a small fraction of putative water–ice depends on the permittivity changes in terms of its dependent parameters, such as the frequency, the temperature, the porosity, and the chemical composition. Our work aims at mitigating false detection or overlooking of water–ce by considering their conditions that previous researches did not cover. We measured the permittivity of different lunar regolith relevant analogue samples with a fixed 40 % porosity in the ultra-high-frequency–super-high-frequency band. We used the coaxial probe method to measure anorthosite, basalt, dunite and ilmenite at$$20\,^\circ \hbox {C}$$20∘C,$$-20\,^\circ \hbox {C}$$-20∘Cand$$-60\,^\circ \hbox {C}$$-60∘C, and we find that, at$$-60\,^\circ \hbox {C}$$-60∘C, the permittivity decreases about 6–18 % compared with the values at$$20\,^\circ \hbox {C}$$20∘C. Within this temperature range, the permittivity is quite similar to the permittivity of water–ice. We find that the conventional calculation would overestimate the permittivity in the low temperature areas, such as the permanently shadowed regions. We also find that each component in the lunar regolith has different temperature-dependent permittivity, which might be important for radar data analysis to detect lunar polar water–ice. Our results also suggest that it should be possible to estimate the water–ice content from radar measurements at different temperatures given an appropriate method.

Graphical Abstract
Middle Miocene forearc alkaline magmatism in Amami-Oshima Island, central Ryukyu Arc: implications for paleoreconstruction of Shikoku Basin
Authors: Motohashi Ginta , Ishizuka Osamu , Oda Hirokuni , Sano Takashi , Sekimoto Shun , Ujiie Kohtaro
Published: January 2023

During the middle Miocene, forearc alkaline magmatism occurred in the Outer Zone of Southwest Japan and the northern Ryukyu Arc, resulting in the formation of forearc alkaline basaltic rocks with ocean island basalt (OIB)-like chemical signatures. In contrast, subduction-related magmatism was present in the central Ryukyu Arc. However, the southwestern margin of the forearc alkaline magmatism was poorly constrained in the Ryukyu Arc. We examined two basaltic dikes in the Chichibu accretionary complex of Amami-Oshima Island, the central Ryukyu Arc. The dikes cut massive basalt, reddish chert, varicolored shale, and grey chert. The chemical compositions of basaltic dikes are characterized by the enrichment of incompatible trace elements, possibly representing a low degree of partial melting from a deep mantle source. 40Ar/39Ar dating analyses indicate that the basaltic dikes yield ages of 16.37 ± 0.14 Ma and 16.51 ± 0.10 Ma. The paleomagnetic analyses on stepwise thermal demagnetizations allowed extracting the direction of stable magnetizations with unblocking temperatures of 450–575 °C for the two dikes [(Dec, Inc) = (138.1°, − 13.3°), (124.0°, − 24.9°)]. The magnetization could be primary, acquired either as part of a secular variation or a geomagnetic excursion during reversed polarity chron/subchron. The 40Ar/39Ar ages and paleomagnetic directions within the error range imply that they intruded almost simultaneously during C5Cn.2r (16.532–16.434 Ma). The age and trace element patterns of the basaltic dikes are comparable to those of Miocene alkaline basaltic rocks, which resulted from forearc alkaline magmatism during the initial subduction of the young and warm Shikoku Basin. The discovery of alkaline basaltic dikes on Amami-Oshima Island suggests that the distribution of middle Miocene forearc alkaline magmatism may extend to the central Ryukyu Arc. Hence, the northern end of the Kyushu-Palau Ridge (i.e., southern end of Shikoku Basin) could have been located south of Amami-Oshima Island around 16.5–16.4 Ma, then moved eastward to the current location.

Graphical Abstract
Partial hepatectomy for a patient with Rendu–Osler–Weber disease: a case report
Authors: Sekiguchi Naoko , Yamada Daisaku , Kobayashi Shogo , Sasaki Kazuki , Iwagami Yoshifumi , Tomimaru Yoshito , Noda Takehiro , Takahashi Hidenori , Doki Yuichiro , Eguchi Hidetoshi
Published: January 2023

Background

Rendu–Osler–Weber disease (Osler disease) is a genetic disease with an autosomal dominant inheritance pattern. It is characterized by widespread telangiectasia in multiple organs. Liver involvement of FNH is relatively common, but liver cancer is very rare, and there are few reports on hepatectomy or postoperative complications. We report a very rare case in which hepatectomy was performed for a patient with Osler disease.

Case presentation

The patient was a 39-year-old man with Osler disease who had been previously diagnosed with multiple FNH and who had been followed for 8 years. During follow-up, the diameter of an S6 lesion gradually increased from 30 to 50 mm; no other lesions increased in size. We decided to perform partial liver resection as total biopsy for the growing tumor, due to the possibility that the growing tumor lesion included malignant components. The pathological examination revealed no obvious malignancy, which was finally diagnosed FNH. The postoperative course was uneventful and he was discharged on the 14th day after surgery. In the second month after discharge, he was transferred to our hospital with sudden abdominal pain in the right hypochondrium with severe tenderness. CT showed extravasation of contrast medium from the hepatic dissection surface in S6, and the hematoma extended to the pelvic floor. Emergency IVR was performed and revealed leakage of the contrast medium from the A6 branch. We embolized the A6 with Lipiodol. After embolization, there were no major problems, and the patient was discharged on the 9th day after the treatment.

Conclusions

Postoperative hemorrhage often occurs within 24 h after surgery, and 2 months after surgery is considered to be the late stage of the wound healing process, and postoperative hemorrhage at this timepoint is considered rare. This unexpected delayed postoperative hemorrhage may have been related to the etiology and pathology of Osler disease, nevertheless, case reports of hepatectomy for patients with Osler disease are limited. We, therefore, report the present case with a review of the relevant literature.
Currencies of greater interest for central Asian economies: an analysis of exchange market pressure amid global and regional interdependence
Authors: Jain Devendra Kumar , Ur-Rehman Naqeeb , Ganiev Omonjon , Arora Kapil
Published: January 2023

Central Asian Economies (CAEs) have diverse exchange rate policies. They have recorded higher volatility in the foreign exchange market since inception. High volatility of the transition era has drifted these economies towards partial dollarization. Monetary authorities in CAEs, (already have a challenge of maintaining monetary policy autonomy) have a gigantic task of price stability and stopping the spread of dollarization. This study is directed towards assessing the drivers and the determinants of foreign exchange market pressure in CAEs. The results, based on panel data analysis and the System GMM model, have provided useful insights about the exchange market pressure determinants particularly USD, Euro, Ruble, and Renminbi. The results show that China and Russia exchange market pressure has a negative effect on the exchange market pressure of CAEs. While the dollar index shows a positive impact on the exchange market pressure of CAEs. Overall, the findings imply that China and Russia currency appreciation results in a trade deficit across CAEs. The policy implication suggests that the floating exchange rate regime (inflation targeting regime) is not in favor of CAEs, and they must use managed-float to reduce their trade deficits.
Empirical evidence on the ownership and liquidity of real estate tokens
Authors: Swinkels Laurens
Published: January 2023

To better understand the potential and limitations of the tokenization of real asset markets, empirical studies need to examine this radically new organization of financial markets. In our study, we examine the financial and economic consequences of tokenizing 58 residential rental properties in the US, particularly those in Detroit. Tokenization aims at fragmented ownership. We found that the residential properties examined have 254 owners on average. Investors with a greater than USD 5,000 investment in real estate tokens, diversify their real estate ownership across properties within and across the cities. Property ownership changes about once yearly, with more changes for properties on decentralized exchanges. We report that real estate token prices move according to the house price index; hence, investing in real estate tokens provides economic exposure to residential house prices.
A modified EDAS model for comparison of mobile wallet service providers in India
Authors: Biswas Sanjib , Pamucar Dragan
Published: January 2023

The present paper has two-fold purposes. First, the current work provides an integrated theoretical framework to compare popular mobile wallet service providers based on users' views in the Indian context. To this end, we propose a new grey correlation-based Picture Fuzzy-Evaluation based on Distance from Average Solution (GCPF-EDAS) framework for the comparative analysis. We integrate the fundamental framework of the Technology Acceptance Model and Unified theory of acceptance and use of technology vis-à-vis service quality dimensions for criteria selection. For comparative ranking, we conduct our analysis under uncertain environments using picture fuzzy numbers. We find that user-friendliness, a wide variety of use, and familiarity and awareness about the products help reduce the uncertainty factors and obtain positive impressions from the users. It is seen that PhonePe (A3), Google Pay (A2), Amazon Pay (A4) and PayTM (A1) hold top positions. For validation of the result, we first compare the ranking provided by our proposed model with that derived by using picture fuzzy score based extensions of EDAS and another widely used algorithm such as The Technique for Order of Preference by Similarity to Ideal Solution. We observe a significant consistency. We then carry out rank reversal test for GCPF-EDAS model. We notice that our proposed GCPF-EDAS model does not suffers from rank reversal phenomenon. To examine the stability in the result for further validation, we carry out the sensitivity analysis by varying the differentiating coefficient and exchanging the criteria weights. We find that our proposed method provides stable result for the present case study and performs better as ranking order does not get changed significantly with the changes in the given conditions.
Coping strategies for happy childless ageing: an explorative study in Poland
Authors: Abramowska-Kmon Anita , Mynarska Monika , Timoszuk Sylwia
Published: January 2023

Childless older people face many challenges resulting from their lack of children that may have an impact on their subjective well-being, especially in a country where family ties are strong, and children are the main source of support. In this paper, we examine the coping strategies that childless seniors in Poland employ in order to mitigate various risks related to having no offspring. We use a qualitative approach, drawing upon in-depth interviews with 42 childless men and women aged 65 or older. We frame our analyses within the life course approach, stressing the biographical development of coping strategies over the life course. Our results corroborate previous findings, and show that the major challenges older childless people in Poland face are related to feeling lonely, and to having insufficient (practical) support and care in case of dependency. We describe the actions the respondents consciously took in response to these challenges, and examine how these actions changed over the life course. The strategies aimed at promoting healthy ageing or helping older people expand their social networks are strongly in line with the respondents’ personal predispositions and earlier lifestyles and experiences.
The role of genetics and epigenetics in sex differences in human survival
Authors: Iannuzzi Vincenzo , Bacalini Maria Giulia , Franceschi Claudio , Giuliani Cristina
Published: January 2023

Sex differences in human survival have been extensively investigated in many studies that have in part uncovered the biological determinants that promote a longer life in females with respect to males. Moreover, researches performed in the past years have prompted increased awareness about the biological effects of environmental factors that can modulate the magnitude of the sex gap in survival. Besides the genetic background, epigenetic modifications like DNA methylation, that can modulate cell function, have been particularly studied in this framework. In this review, we aim to summarize the role of the genetic and epigenetic mechanisms in promoting female advantage from the early in life (“INNATE” features), and in influencing the magnitude of the gap in sex differences in survival and ageing (“VARIABLE” features). After briefly discussing the biological bases of sex determination in humans, we will provide much evidence showing that (i) “innate” mechanisms common to all males and to all females (both genetic and epigenetic) play a major role in sex differences in lifespan; (ii) “variable” genetic and epigenetic patterns, that vary according to context, populations and exposures to different environments, can affect the magnitude of the gap in sex differences in survival. Then we will describe recent findings in the use of epigenetic clocks to uncover sex differences in biological age and thus potentially in mortality. In conclusion, we will discuss how environmental factors cannot be kept apart from the biological factors providing evidence from the field of human ecology.
Post-mortem freshwater animal predation—a case report
Authors: S. H. Jayanth , K. B. Geetha , Vijay Vidusha , Prakash Manju
Published: January 2023

Background

Post-mortem animal predation affects human corpses in various ways. Lesions are a characteristic of the type of terrestrial or aquatic predators. These lesions can be misinterpreted and may be challenging for the forensic pathologist. Different species in freshwater cause different types of lesions depending on their feeding habits. There is a paucity in the literature about post-mortem lesions caused by freshwater animals.

Case presentation

A 24-year-old man had accidentally drowned while fishing in the River Cauvery. His body was recovered after 3 days. Though the cause of death was drowning, post-mortem animal activity was observed during autopsy, and a live juvenile crab was also found along with the body. Distinct post-mortem lesions caused by decapods Oziotelphusa wagrakarowensis and small fishes Hypselobarbus dubius and Dawkinsia arulius are described.

Conclusions

Aquatic predators and their activity on the corpse are different from that of terrestrial predators. Knowledge of the local fauna is required to correlate the lesions on the body to the predator.
Quantitative analysis of the innominate bone for sex estimation utilising the Phenice method
Authors: Hamzah Muhammad Naqib Harith , Ali Siti Hanum Mohd , Nor Faridah Mohd , Hadi Helmi
Published: January 2023

Background

The Phenice method is a reliable qualitative analysis of the pubis and ischium region of the innominate bone to assess an individual’s sex. Geometric morphometric provides methods of quantitative analysis of the region for identification purposes, which may be affected by environmental changes. This study analysed the Phenice method by utilising 3D (3 dimensional) CT (computed tomography) scans by geometric morphometrics for sex estimation. The CT scans of 231 adult individuals (148 males and 83 females) of the Malaysian population were landmarked by IDAV Landmark software and analysed by MorphoJ and SPSS.

Results

The first four principal components (PCs) accounted for 41.9% of the total changes when utilising the Phenice method. Widening in pubic symphysis in a U shape with a general widening of the border of the true pelvis, which is typically seen in females, accounted for 21.8% of the eigenvalues.

Conclusions

It was possible to quantify the changes seen in the ventral arc, subpubic concavity and the medial aspect of the ischio-pubic ramus for sex estimation utilising the Phenice method, and most of the changes were seen in PC1 at the pubic region, which accounted for 21.8%.
Nonlinear analysis of beat-to-beat variability of action potential time series data identifies dynamic re-entrant substrates in a hypokalaemic mouse model of acquired long QT syndrome
Authors: Tse Gary , Zhou Jiandong , Dong Xiuming , Hao Guoliang , Lee Sharen , Leung Keith Sai Kit , Leung Fung Ping , Liu Tong , Du Yimei , Cheng Shuk Han , Wong Wing Tak
Published: January 2023

Background

Previous studies have quantified repolarization variability using time-domain, frequency-domain and nonlinear analysis in mouse hearts. Here, we investigated the relationship between these parameters and ventricular arrhythmogenicity in a hypokalaemia model of acquired long QT syndrome.

Methods

Left ventricular monophasic action potentials (MAPs) were recorded during right ventricular regular 8 Hz pacing during normokalaemia (5.2 mM [K+]), hypokalaemia modeling LQTS (3 mM [K+]) or hypokalaemia with 0.1 mM heptanol in Langendorff-perfused mouse hearts.

Results

During normokalaemia, mean APD was 33.5 ± 3.7 ms. Standard deviation (SD) of APDs was 0.63 ± 0.33 ms, coefficient of variation was 1.9 ± 1.0% and the root mean square (RMS) of successive differences in APDs was 0.3 ± 0.1 ms. Low- and high-frequency peaks were 0.6 ± 0.5 and 2.3 ± 0.7 Hz, respectively, with percentage powers of 38 ± 22 and 61 ± 23%. Poincaré plots of APDn+1against APDnrevealed ellipsoid morphologies with SD along the line-of-identity (SD2) to SD perpendicular to the line-of-identity (SD1) ratio of 4.6 ± 1.1. Approximate and sample entropy were 0.49 ± 0.12 and 0.64 ± 0.29, respectively. Detrended fluctuation analysis revealed short- and long-term fluctuation slopes of 1.62 ± 0.27 and 0.60 ± 0.18, respectively. Hypokalaemia provoked ventricular tachycardia in six of seven hearts, prolonged APDs (51.2 ± 7.9 ms), decreased SD2/SD1 ratio (3.1 ± 1.0), increased approximate and sample entropy (0.68 ± 0.08 and 1.02 ± 0.33) and decreased short-term fluctuation slope (1.23 ± 0.20) (ANOVA,P < 0.05). Heptanol prevented VT in all hearts studied without further altering the above repolarization parameters observed during hypokalaemia.

Conclusion

Reduced SD2/SD1, increased entropy and decreased short-term fluctuation slope may reflect arrhythmic risk in hypokalaemia. Heptanol exerts anti-arrhythmic effects without affecting repolarization variability.
Student interaction discourse moves: characterizing and visualizing student discourse patterns
Authors: Nennig Hannah T. , States Nicole E. , Montgomery Marika T. , Spurgeon Sidney G. , Cole Renée S.
Published: January 2023

Student-centered instruction allows students to take ownership over their learning in the classroom. However, these settings do not always promote productive engagement. Using discourse analysis, student engagement can be analyzed based on how they are interacting with each other while completing in-class group activities. Previous analyses of student engagement in science settings have used methods that do not capture the intricacies of student group interactions such as the flow of conversation and nature of student utterances outside of argumentation or reasoning. However, these features are important to accurately assess student engagement. This study proposes a tiered analytical framework and visualization scheme for analyzing group discussion patterns that allow for a detailed analysis of student discourse moves while discussing scientific topics. This framework allows a researcher to see the flow of an entire conversation within a single schematic. The Student Interaction Discourse Moves framework can be used to extend studies using discourse analysis to determine how student groups work through problems.
Tongue base schwannoma: case presentation and literature review
Authors: Ouattassi Naouar , Labiyed Mehdi , Toubi Zakaria , Zaki Zouheir , El Alami Mohamed Nouredine El Amine
Published: January 2023

Background

Schwannoma, also known as perineural fibroblastoma, neuroma, or neurilemmoma, is a slow growing benign tumor that exceptionally raises in oral cavity. It mainly affects the second and third decade and can be life-threatening if it becomes large. Developing in youth is unusual.

Case presentation

A 16-year-old teenager presenting with relatively rapidly growing tongue base tumor which radiologic investigations revealed features of benign tumor, surgery was performed through a standard transoral approach and pathology with immunohistochemistry examination confirmed the diagnosis of schwannoma with no evidence of malignant transformation.

Conclusion

Oral cavity schwannoma is rare, and the prevailing oral location is the tongue. This particular site holds many risks related to impact symptoms or to anesthesia and securing airways. We performed a trans-oral resection of a tongue base schwannoma using a cold instrument. As the tumor is well encapsulated, this approach seems convenient and less invasive for complete surgical excision.
Digital ECMT Cancer Trial Matching Tool: an Open Source Research Application to Support Oncologists in the Identification of Precision Medicine Clinical Trials
Authors: O'Regan Paul , Hoskins Richard , Grave Christopher , Stevenson Julie-Anne , Frost Hannah , Graham Donna M. , Krebs Matthew G. , Freitas Andre , Landers Dónal
Published: January 2023

PURPOSE

Matching patients with cancer to precision medicine clinical trials on the basis of their tumor genotype has the potential to improve outcomes for patients who have exhausted standard-of-care treatment options. However, the matching process presents a substantial challenge because of the number of clinical trials available. We describe a free, open source research tool designed to extract relevant trial information to support oncologists in the matching process, and we illustrate its utility with recent case studies of patients who were matched to trials using this tool.

METHODS

Trial records are sourced from ClinicalTrials.gov and indexed using natural language processing techniques, including named entity recognition, term normalization, and relationship extraction. Relationships between trials and genetic alterations are assigned scores on the basis of a rule-based system. All data are updated daily. A user interface is provided via R Shiny app.

RESULTS

An instance of the trial match tool, configured for UK clinical trials, is hosted by the digital Experimental Cancer Medicine Team (see link in Data Sharing Statement). Users select the relevant cancer type and genetic alteration(s). Matching studies are ranked according to the score assigned for the selected genetic alterations. Results may be downloaded and attached to the patient's health record if desired. The tool is currently being used to support the ongoing TARGET National study, which aims to match up to 6,000 patients to early phase clinical trials. We present three case studies that exemplify relationships between genetic alterations and studies.

CONCLUSION

With increasing numbers of precision medicine treatments and as comprehensive molecular profiling of tumor samples becomes more common, decision support tools are likely to become increasingly important. This work represents an important step toward the development and wider implementation of such systems.
Understanding the Role of Urology Practice Organization and Racial Composition in Prostate Cancer Treatment Disparities
Authors: Agochukwu-Mmonu Nnenaya , Qin Yongmei , Kaufman Samuel , Oerline Mary , Vince Randy , Makarov Danil , Caram Megan V. , Chapman Christina , Ravenell Joseph , Hollenbeck Brent K. , Skolarus Ted A.
Published: January 2023

PURPOSE:

Black men have a higher risk of prostate cancer diagnosis and mortality but are less likely to receive definitive treatment. The impact of structural aspects on treatment is unknown but may lead to actionable insights to mitigate disparities. We sought to examine the associations between urology practice organization and racial composition and treatment patterns for Medicare beneficiaries with incident prostate cancer.

METHODS:

Using a 20% sample of national Medicare data, we identified beneficiaries diagnosed with prostate cancer between January 2010 and December 2015 and followed them through 2016. We linked urologists to their practices with tax identification numbers. We then linked patients to practices on the basis of their primary urologist. We grouped practices into quartiles on the basis of their proportion of Black patients. We used multilevel mixed-effects models to identify treatment associations.

RESULTS:

We identified 54,443 patients with incident prostate cancer associated with 4,194 practices. Most patients were White (87%), and 9% were Black. We found wide variation in racial practice composition and practice segregation. Patients in practices with the highest proportion of Black patients had the lowest socioeconomic status (43.1%), highest comorbidity (9.9% with comorbidity score ≥ 3), and earlier age at prostate cancer diagnosis (33.5% age 66-69 years; P < .01). Black patients had lower odds of definitive therapy (adjusted odds ratio, 0.87; 95% CI, 0.81 to 0.93) and underwent less treatment than White patients in every practice context. Black patients in practices with higher proportions of Black patients had higher treatment rates than Black patients in practices with lower proportions. Black patients had lower predicted probability of treatment (66%) than White patients (69%; P < .05).

CONCLUSION:

Despite Medicare coverage, we found less definitive treatment among Black beneficiaries consistent with ongoing prostate cancer treatment disparities. Our findings are reflective of the adverse effects of practice segregation and structural racism, highlighting the need for multilevel interventions.
Maternal Exercise Protects Male Offspring From Maternal Diet–Programmed Nonalcoholic Fatty Liver Disease Progression
Authors: Hinrichs Holly , Faerber Austin , Young Monica , Ballentine Samuel J , Thompson Michael D
Published: January 2023

Maternal obesity programs the risk for development of nonalcoholic fatty liver disease (NAFLD) in offspring. Maternal exercise is a potential intervention to prevent developmentally programmed phenotypes. We hypothesized that maternal exercise would protect from progression of NAFLD in offspring previously exposed to a maternal obesogenic diet. Female mice were fed chow (CON) or high fat, fructose, cholesterol (HFFC) and bred with lean males. A subset had an exercise wheel introduced 4 weeks after starting the diet to allow for voluntary exercise. The offspring were weaned to the HFFC diet for 7 weeks to induce NAFLD. Serum, adipose, and liver tissue were collected for metabolic, histologic, and gene expression analyses. Cecal contents were collected for 16S sequencing. Global metabolomics was performed on liver. Female mice fed the HFFC diet had increased body weight prior to adding an exercise wheel. Female mice fed the HFFC diet had an increase in exercise distance relative to CON during the preconception period. Exercise distance was similar between groups during pregnancy and lactation. CON-active and HFFC-active offspring exhibited decreased inflammation compared with offspring from sedentary dams. Fibrosis increased in offspring from HFFC-sedentary dams compared with CON-sedentary. Offspring from exercised HFFC dams exhibited less fibrosis than offspring from sedentary HFFC dams. While maternal diet significantly affected the microbiome of offspring, the effect of maternal exercise was minimal. Metabolomics analysis revealed shifts in multiple metabolites including several involved in bile acid, 1-carbon, histidine, and acylcarnitine metabolism. This study provides preclinical evidence that maternal exercise is a potential approach to prevent developmentally programmed liver disease progression in offspring.
Field model for multistate lateral diffusion of various transmembrane proteins observed in livingDictyosteliumcells
Authors: Takebayashi Kazutoshi , Kamimura Yoichiro , Ueda Masahiro
Published: January 2023

The lateral diffusion of transmembrane proteins on plasma membranes is a fundamental process for various cellular functions. Diffusion properties specific for individual protein species have been extensively studied, but the common features among protein species are poorly understood. Here, we systematically studied the lateral diffusion of various transmembrane proteins in the lower eukaryote Dictyostelium discoideum cells using a hidden Markov model for single-molecule trajectories obtained experimentally. As common features, all membrane proteins that had from one to ten transmembrane regions adopted three free diffusion states with similar diffusion coefficients regardless of their structural variability. All protein species reduced their mobility similarly upon the inhibition of microtubule or actin cytoskeleton dynamics, or myosin II. The relationship between protein size and the diffusion coefficient was consistent with the Saffman–Delbrück model, meaning that membrane viscosity is a major determinant of lateral diffusion, but protein size is not. These protein species-independent properties of multistate free diffusion were explained simply and quantitatively by free diffusion on the three membrane regions with different viscosities, which is in sharp contrast to the complex diffusion behavior of transmembrane proteins in higher eukaryotes.