bluuebunny/crossref_metadata_2025 huggingface dataset:
HISTOLOGICAL STRUCTURE OF THE RAT FEMURS AFTER FILLING OF DEFECTS IN THE DISTAL METAPHYSIS WITH 3D-PRINTED IMPLANTS BASED ON POLYLACTIDE AND TRICALCIUM PHOSPHATE IN COMBINATION WITH MESENCHYMAL STROMAL CELLS
Authors: Ashukina Nataliya , Gontar Nazar , Danуshchuk Zinaida , Nikolchenko Olga , Kaliyuzhna Yaryna
Published: October 2023

Polylactide (PLA) frameworks printed on a 3D printer are used for filling the bone defects. The osteotropic properties of 3D-PLA can be improved by combining with tricalcium phosphate (TCP) and mesenchymal stromal cells (MSCs). Objective. Study the reconstruction in the rat femurs after implanting 3D-printed implants based on PLA and TCP (3D-I) in combination with cultured allogeneic MSCs into defects in the distal metaphysis. Methods. 48 white laboratory rats (age 5–6 months) were used, which were randomly divided into groups: Control — 3D-I; Experiment-I — 3D-I, saturated MSCs; Experiment II — 3D-I, with injection of 0.1‒0.2 ml of medium with MSCs into the area of surgical intervention 7 days after implantation. 15, 30 and 90 days after the operation, histological (with histomorphometry) studies were conducted. Results. The area of 3D-I decreased with time in all groups and connective and bone tissues formed in different ratios. 15 days after the surgery, in the Experiment-I group, the area of the connective tissue was 1.9 and 1.6 times greater (p<0.001) in comparison to the Control and Experiment II; 30 days it was greater 1.6 times (p < 0.001) and 1.4 times (p=0.001), respectively. 30 days after the surgery, the area of newly formed bone in the Experiment-I group was 2.2 times (p < 0.001) less than in the Control. On the contrary, in the Experiment-II, the area of newly formed bone was 1.5 and 3.3 times greater (p < 0.001) compared to Experiment-I and Control, respectively. Conclusions. The studied 3D-I with time after their implantation into the metaphyseal defects of the rats’ femurs are replaced by connective and bone tissues. The use of 3D-I, saturated MSCs, 15 and 30 days after the surgery, caused excessive formation of connective tissue and slower bone formation. Local injection of MSCs 7 days after the implantation of 3D-I caused to the formation of a larger area of newly bone 30th day after surgery compared to 3D-I alone and 3D-I with MSCs.
MORPHOLOGICAL COMPARISON OF THE STROMAL-VASCULAR FRACTION OF THE SUBCUTANEOUS FAT CELL AND INFRAPATELLAR FAT PAD
Authors: Maslenikov Serhiy , Avramenko Yulia , Golovakha Maxim
Published: October 2023

The use of regenerative technologies is widespread in modern medicine. Adipose-derived stem cells (ADSCs) in the stromalvascular fraction (SVF) appear to be most attractive for use in cell therapy. The objective of this study is to identify morphological differences in the stromal-vascular fraction from the subcutaneous tissue and infrapatelar fat pad (IFP). Material and methods. Data analysis of 15 patients was carried out. The average age of the patients was (44.0 ± 3.8) years with a body mass index of (20.1 ± 1.6) kg/m2 . Aspirates of subcutaneous adipose tissue were obtained from the anterior abdominal wall of 8 women without signs of obesity and comorbid metabolic diseases, as well as the resected IFP from 7 patients during therapeutic and diagnostic arthroscopy, also without signs of obesity. We conducted a comparative characterization of the composition of the obtained histological samples. Morphometric measurements of the diameter of adipocytes were performed. Parametric and non-parametric criteria of statistical analysis were applied. Parametric criteria were used to determine the average diameter of adipocytes and the standard deviation. Nonparametric ones were used to test the hypothesis about the normality of the distribution of the studied indicators according to the Shapiro–Wilk test. The results. SVF, which were obtained in our study from subcutaneous adipose tissue and from IFP, differ in composition, namely, the amount of the stromal component, which is visually much more pronounced in the SVF of IFP, the size of adipocytes, which in SVF from subcutaneous adipose tissue of a statistically larger diameter. Conclusions. It was morphologically proven that SVF of IFP is a promising source of adipose mesenchymal stem cells for regenerative medicine, especially for cartilage regeneration.
TREATMENT OF NEGLECTED COMPLEX DISLOCATIONS IN THE ELBOW JOINT (CLINICAL CASE)
Authors: Matelenok Yevgen
Published: October 2023

Neglected complex dislocations of the elbow joint are not often pathology, bat its treatment is serious problem and demand an individual tactic of treatment. Objective. To present a clinical case of consecutive treatment the neglected complex dislocations of the elbow. Methods. The clinical case of neglected complex dislocations to the back with coronoid process fracture II type (by Regan, Morrey) with displacement to a 60-year old man, who could not get medical care within 6 months. At the moment of examination patient had formed steadfast interrelations of displaced elbow joint structures with loosing of limb functionality. At the first stage, the forearm was distracted by using an external fixation device end redaction in the shoulder-elbow joint was achieved. At another stage arthrolysis, open redaction of the radial head, restoration of the lateral ligamentous apparatus was complected. In three weeks restoration of movements in the elbow joint has begun, ensuring movements close to the natural axis of the forearm rotation, which was provided by the external fixation device. Results. In 6 months the patient noted moderate pain only after intense physical load, hi doesn’t take painkillers, volume o f rotational m ovements: 2 0/0/25 (45°), e xtension-flexion movements: 0/15/118 (103°). The patient actively uses the limb for self-care and in work activities. According to the Mayo Elbow Performance Score the sum of points is 75, this means — the result is good. Conclusions. In cases of neglected complex dislocations for significant periods of existence (more than 3–4 months) staged treatment tactics is appropriate with using external fixation devices, by perforce perform open and closed manipulations, which depends on the specific clinical situation.
Analyzing features of measles immune response in adult patients
Authors: Toptygina Anna P. , Mamaeva Tamara A.
Published: October 2023

Measles is a highly contagious, vaccine-preventable anthroponotic infection. Despite the policy of active measles vaccination for entire global population, measles has not completely disappeared, it wanes and waxes in outbreaks. It has been shown that not only the unvaccinated but also the vaccinated, which is quite understandable, subjects are involved in measles progression. The study was aimed at assessing features of the immune response in adult measles patients at rise in disease incidence upon its eradication. 1.158 blood sera from measles patients aged 18 to 70 living in Moscow and the Moscow region were studied by ELISA (Euroimmun, Germany). The vast majority of subjects were aged 1840 years comprising 72.36% cases. 69 sera collected at a later timepoint (1020 days from rash onset) were excluded and analyzed separately. According to anti-measles IgG and avidity level, the data were stratified as follows: group 1 with a primary type of immune response 582 sera (53.44%), not vaccinated in childhood; group 2 (secondary response) 446 sera (40.96%), vaccinated in childhood, but lost anti-measles antibodies; group 3 with intermediate level of parameters 61 sera. To clarify the type of immune response in group 3, an additional analysis of the spectrum of anti-measles antibody subclasses was carried out. It was shown that a mixed type of response was detected in this group: 31 subjects mainly had primary immune response, and 30 subjects secondary immune response. At the same time, 40 out of 61 subjects were vaccinated against measles 13 months prior to disease onset, i.e., it was overlapped on arising post-vaccination immunity. Thus, the previously identified trend towards the loss of post-vaccination immunity among older schoolchildren and young adults vaccinated in childhood who are involved in the epidemic process continues to worsen.
Biological characteristics of the enteroaggregative escherichia coli ont:h30 18-726 (no. B-8857) strain isolated from a patient with ulcerative colitis and a new method of pcr identification
Authors: Makarova Maria A. , Kruglov Egor E. , Kaftyreva Lidia A.
Published: October 2023

The aim of the work is to assess biological properties of the enteroaggregativeEscherichia coli(EAgEC) strain isolated from the colon mucosa biopsy material from a patient with ulcerative colitis, and to develop a new method for PCR identification ofE. colistrains.
Materials and methods.The study involved 46 patients with a verified ulcerative colitis. Isolation of 87E. colistrains was carried out by using colon biopsy material obtained during a standard endoscopic procedure. The description of the biochemical bacterial properties was combined with the molecular genetic detection of virulence determinants and presence of antibiotic resistance mechanisms. Using the “AmpliSense®Escherichiose — FL” reagent kit, a screening for the presence of diarrheagenicE. colistrains was performed, which revealed the presence of a single strain of EAgEC. The methodological approach to creating a new method for strain identification was based on the search for a unique genetic sequence within the glutamate decarboxylase(gad)gene.
Results.E. coli18-726 can be described biochemically typical to its species. The studied strain was characterized by a multiple resistance phenotype (MDR) to antibiotics, as well as a lack of sensitivity to five bacteriophages. TheE. coli18-726 strain had a unique sequence of the gene encoding the O-antigen, which differed from 188 known O-antigens (ONT) and, by the identity of the nucleotide sequence of the gene encoding the synthesis of the H-antigen, the strain belonged to the H30 serovar. Thus, the antigenic formula of strain EAgEC 18-726 was expressed as ONT:H30. TheE. coli18-726 strain contained a significant spectrum of genes that enable properties of virulence(iss, capU, aggA, aggB, aggC, aggD, aap, aar)and resistance to antibiotics(blaCTX-M-15, blaTEM-1B, aadA1, aadA5, mph(A), catB3). A new method for identifying EAgEC in chronic bowel disease is based on the polymerase chain reaction method using primers for a specific region of bacterial glutamate decarboxylase(gad)gene.Conclusion.1) The biochemical, antigenic and molecular genetic properties ofE. coliONT:H30 18-726 strain (No. B-8857) isolated from a patient with histomorphologically diagnosed ulcerative colitis were analyzed. 2) A method for PCR identification of EAgEC strains based on the detection of a specific region within the bacterial genomic glutamate decarboxylase gene has been created and patented.
Biochemical markers assessing rate of reparative processes in patients with infiltrative pulmonary tuberculosis
Authors: Esmedlyaeva Dilyara S. , Alekseeva Nina P. , Dyakova Marina  E.
Published: October 2023

Theeffectiveness of chemotherapy for infiltrative pulmonary tuberculosis (ITL) is determined by proper timing and adequacy of treatment. The speed of reparative changes in the pulmonary parenchyma is associated with the effectiveness of therapy. Different classes of proteinases are involved in extracellular matrix remodeling. The aim of the study was to evaluate a potential of using the markers of proteinase/inhibitors axis for predicting effectiveness of therapy in patients with ITL due to varying resistance ofMycobacterium tuberculosisto anti-tuberculosis drugs (PTP).Materials and methods.A retrospective study included 60 and 55 ITL patients with the drug sensitive (DS) and drug resistance (DR) Mtb strains, respectively. Patients were divided into two groups according to therapy effectiveness. The levels of blood serum matrix metalloproteinase MMP-1, MMP-3, MMP-8, MMP-9 and their TIMP-1 inhibitor, neutrophil elastase (NE), alpha-2 macroplobulin (MG) and proteinase inhibitor (PI) were determined. Statistica 10.0 and R software packages were used.Results. According to the model of discriminant analysis, the pattern of host inflammatory response in ITL was accounted for not only by varying pathogen resistance, but also by the different size of lung damage. Patients with a limited and widespread process with the DS of the pathogen strains were assigned to group I and II, and DR — to group III and IV group, respectively. The decisive in the speed of tissue repair was the number of neutrophils bearing various levels of proteinase MMP-8, MMP-9 (I group) or TIMP-1 and PI inhibitors (II group). Only combining biochemical data with the those on radiation methods are possible for prognosis in a group of patients with DR (III and IV).Conclusions.Combinations of the markers of the proteinase/inhibitors axis are informative in assessing a rate of reparative changes in ITL and differ not only depending on the pathogen DS strains, but also from the size of destructed pulmonary parenchyma. Differences in parameter combinations are accounted for by history of a specific disease.
Perspectives for applying Alphaviruses in antitumor therapy
Authors: Nazarenko Alina S. , Biryukova Yulia K. , Kolyasnikova Nadezhda M , Vorovich Mikhail F. , Pestov Nikolai B. , Ishmukhametov Aidar A.
Published: October 2023

Oncolytic viral therapy is a promising approach for treating tumors. Oncolytic viruses can directly lyse tumor cells and indirectly activate antitumor immunity. Alphaviruses, as oncolytic viruses, are particularly promising agents because they can selectively infect and lyse tumor cells, modulate microtumor environment, elicit immune-mediated lysis of tumor cells, and serve as a platform for transgene delivery. To ensure safety, attenuated strains of Alphaviruses are typically used for genetic engineering, and immunogenic tumor-associated antigens or cytokines are commonly chosen as transgenes. Studies evaluating both in vitro and in vivo oncolytic and immunomodulatory effects of Alphaviruses and vectors based on them have been growing exponentially. Animal models of various tumor types were used to examine the effectiveness of Alphaviruses, including Sindbis, Semliki Forest virus, Geta (strain M1), Venezuelan equine encephalitis virus, and vectors based on them. Additionally, Alphaviruses revealed enhanced antitumor activity while used in combination therapies with other oncolytic viruses. Alphavirus-like replicon particles based on attenuated Venezuelan equine encephalitis virus may serve for transgene delivery to express heterologous proteins at high levels, and induce both humoral and cellular immune responses. An alphaviral vector-based vaccine, encoding the HER2 extracellular and transmembrane domains, has demonstrated safety and efficacy in preclinical mouse models, as well as in phase I clinical trials for advanced breast cancer patients with HER2 overexpression. This vaccine is known to be safe, effective, and capable of inducing T-cell immunity. In this review, we discuss the current progress in preclinical and clinical investigations, as well as the future potential of Alphaviruses for oncolytic virotherapy.
Construct Validity of the Brazilian Version of the Dyadic Adjustment Scale
Authors: Hernandez José Augusto Evangelho , Peçanha Raphael Fischer , Soares Laura Costa Mattos , Lima Isabela da Costa Nascimento de
Published: October 2023

Abstract This study sought evidence of construct validity for the Brazilian versions of the Dyadic Adjustment Scale and Revised Dyadic Adjustment Scale. A total of 448 individuals participated in the research, 253 women and 195 men from several regions of Brazil who had been cohabiting with their marital partners for an average of 14.7 years. Several proposed models for the measure were tested in Structural Equation Modeling. In the Confirmatory Factor Analysis, the four-factor and hierarchical models of the Revised Dyadic Adjustment Scale showed good overall adjustments. Evidence of factor, convergent, and discriminant validity were also found. Composite reliability revealed adequate levels of internal consistency. The Multigroup Confirmatory Factor Analysis demonstrated a strong measurement invariance model for men and women.
PRÁTICAS DE LIDERANÇA EM EMPRESAS FAMILIARES: UM OLHAR FENOMENOGRÁFICO
Authors: Meurer Tatiane , Beck Franciele
Published: October 2023

RESUMO O estudo busca compreender como os tops managers entendem e praticam o estilo de liderança nas empresas familiares, em que aplicou-se uma metodologia interpretativista, a fenomenografia, guiada por entrevistas semiestruturadas com 15 tops managers. A análise fenomenográfica visa identificar dois grupos de estilos predominantes: i) liderança conciliadora, dinâmico; e ii) liderança regrada, estático. Em particular, no primeiro grupo, têm-se a concatenação de três estilos de liderança: referente, especialista e participativo, que compartilham semelhanças entre si, reverberando um ambiente organizacional afetivo e coletivo, o qual impacta no estilo de liderança adotado pela organização. Enquanto, a liderança autocrática é representada isoladamente no segundo grupo, dado os aspectos organizacionais, ressoando um ambiente metódico e hierarquizado, elucidando práticas de lideranças estáticas. Contudo, compreende-se que há variabilidade no estilo de liderança das empresas familiares, sendo que esse estilo advém do envolvimento da família, em que os valores e a conduta organizacional exposta pelos fundadores reporta os traços de comportamento dos líderes com seus subordinados. As contribuições teóricas revelam o avanço conceitual para a literatura de estilo de liderança, no que concerne à definição de estilo de liderança sob o olhar dos tops managers, lançando luz para a heterogeneidade encontrada nas empresas familiares por meio do estilo de liderança.
Denegrindo trajetórias acadêmicas: formação docente em Química e a Lei 10.639/2003
Authors: Camargo Marysson Jonas Rodrigues , Faustino Gustavo Augusto Assis , Benite Anna Maria Canavarro
Published: October 2023

Resumo O racismo que engendrou todo tipo de sofismas para difundir a tese de que a África e sua diáspora são constituídas de seres inferiores modulou um currículo que o produz e reproduz em sala de aula. Em contrapartida, as populações negras têm resistido. Na esteira desse movimento contra hegemônico, este artigo teve como objetivo conhecer e analisar o desenvolvimento e planejamento de uma intervenção pedagógica em um curso de formação de professores de Química, na disciplina Educação para as Relações Étnico-Raciais no Ensino de Ciências-Química, desenvolvida pelo Coletivo X. O trabalho, caracterizado como pesquisa-ação emancipatória, coligiu dados empíricos por registro fílmico, que foram analisados conforme elementos de uma análise bakhtiniana. Os resultados permitiram reconhecer a responsividade dos licenciandos nas discussões levantadas, a polifonia da natureza dos enunciados e concluir sobre uma habilidade afroperspectivista, que os docentes terão que desenvolver para denegrir o ensino de Química, denominada de polirracionalidade.
Overall survival and associated factors in women with metastatic breast cancer treated with trastuzumab at a public referral institution
Authors: Gonçalves Débora Silva , Santos Arn Migowski Rocha dos , Costa Susanne Crocamo Ventilari da , Costa Rodrigo Saar da , Senna Katia Marie Simões e , Zimmermann Ivan Ricardo
Published: October 2023

ABSTRACT Objective: To characterize associated factors and overall survival of women with metastatic breast cancer treated with trastuzumab after its incorporation into the SUS, and additionally to present the direct costs of this technology. Methods: This is a retrospective cohort, based on data from computerized medical records from one of the units of the National Cancer Institute (INCA), in Rio de Janeiro-RJ, Brazil. Women with HER-2 positive metastatic breast cancer undergoing trastuzumab treatment from September 2017 to August 2018 were included. Overall survival was estimated using the Kaplan-Meier method and compared between groups using the log-rank test. Results: 136 women were selected, whose median age at diagnosis was 51 years (range: 21–81 years). The median OS was 43.63 months (95%CI 33.92–53.34). It is observed that the median OS for the population already diagnosed with metastatic disease (stage IV) was significantly lower than for patients diagnosed in stages I-III (37.43 months vs. 48.6 months, p<0, 01). Women without previous use of trastuzumab had a higher median OS than patients pretreated with trastuzumab (45.16 months vs. 40.73 months, p<0.01). Conclusion: Trastuzumab improves survival in HER-2 positive metastatic breast cancer. Brain and multiple metastases are associated with a worse prognosis. It is essential to avoid advanced staging and perform surgical treatment, with emphasis on radical mastectomy. The SUS must adopt policies and strategies for early diagnosis and guarantee access to trastuzumab, considering its high cost.
Cobertura vacinal da tríplice viral e poliomielite no Brasil, 2011-2021: tendência temporal e dependência espacial
Authors: Palmieri Isadora Gabriella Silva , Lima Lucas Vinícius de , Pavinati Gabriel , Silva José Arthur Paschoalotto , Marcon Sonia Silva , Sato Ana Paula Sayuri , Magnabosco Gabriela Tavares
Published: October 2023

RESUMO Objetivo: Analisar a cobertura das vacinas tríplice viral e contra poliomielite, a tendência temporal e a dependência espacial em crianças de até um ano no Brasil, entre 2011 e 2021. Métodos: Estudo ecológico com dados secundários das taxas de cobertura vacinal (CV), disponibilizadas pelo Sistema de Informação do Programa Nacional de Imunização. A análise de tendência ocorreu pelo método joinpoint, segundo regiões geográficas, estimando a variação percentual anual (APC) e seu respectivo intervalo de confiança (IC95%). Foram construídos mapas coropléticos de distribuição por região de saúde e, posteriormente, verificou-se a dependência espacial pela estatística de Moran. Resultados: Entre 2011 e 2021, as coberturas vacinais apresentaram queda no Brasil, tanto para tríplice viral (APC: −6,4%; IC95%: −9,0; −3,8) quanto para poliomielite (APC: −4,5%; IC95% −5,5; −3,6). Houve declínio da cobertura de ambas as vacinas em todas as regiões geográficas ao longo dos anos de estudo, exceto no Sul e no Centro-Oeste para a vacina tríplice viral. Desde 2015, poucas regionais de saúde do país atingiram a CV adequada (≥95 a <120%). As regiões sanitárias do Norte e do Nordeste apresentaram clusters do tipo baixo-baixo na análise univariada para ambos os imunobiológicos. Conclusão: É premente considerar estudos como este para o planejamento de estratégias mais eficazes à imunização de crianças, sobretudo em áreas de maior queda. Desse modo, pode-se romper as barreiras do acesso à imunização, dada a heterogeneidade brasileira, e ampliar o acesso a informações fidedignas que aumentem a confiança na eficácia vacinal.
CLASSES DE PALAVRAS E DURAÇÃO DE PAUSAS PREENCHIDAS EM PACIENTES COM ESQUIZOFRENIA
Authors: COSTA José Carlos , SILVA Luis Filipe Lima e
Published: October 2023

RESUMO O objetivo deste trabalho é comparar a duração e distribuição de pausas preenchidas, que possuem um segmento vocalizado, em relação a classes de palavras de pacientes com esquizofrenia e pessoas sem essa condição. As hipóteses deste trabalho são: I) existe diferença significativa na duração de pausas preenchidas entre pacientes e não pacientes II) existe diferença significativa entre a duração de pausas diante de certas classes de palavras entre os dois grupos. Metodologicamente, são comparadas 112 pausas preenchidas, extraídas de 13 áudios do C-ORAL-ESQ (Rocha, 2019), corpus representativo da fala de pacientes com esquizofrenia e 11 áudios do C-ORAL-BRASIL I (Raso; Mello, 2012), corpus representativo do português brasileiro falado informal. Para classificar as classes de palavras, foi utilizado um etiquetador do tipo brill tagger treinado no Mac-Morpho (Aluísio et al., 2003) com 92,24% de acurácia e adaptado para dados orais por meio de normalização ortográfica em linguagem Python. Os resultados mostram que há diferença estatística significativa (p = 0,003) entre a duração de pausas preenchidas entre pacientes e não pacientes. Em relação às classes de palavras, foi observado que os pacientes realizam pausas preenchidas maiores na maioria das classes de palavras, exceto em advérbios, mas há diferença relevante apenas entre pausas diante de pronomes pessoais (p = 0,007).
Multivariate analysis applied to the evaluation of genetic variability for the physiological quality trait of common bean seeds
Authors: Silva Priscila Carvalho da , Lima Natalia Dias , Masetto Tathiana Elisa , Gonçalves Manoel Carlos , Cândido Liliam Silvia
Published: October 2023

Abstract: Knowledge of the genetic variability and of the variables for evaluating common bean lines for the physiological seed quality trait is important for the selection of promising common bean genotypes for this trait in breeding programs. Through multivariate analysis, the objective of this study was to evaluate the efficiency of physical and physiological tests for studies of genetic variability in seeds of common bean lines. Twenty lines of common bean were evaluated for thousand-seed weight, first count, germination, tetrazolium, electrical conductivity, accelerated aging, field emergence and seedling performance. The experimental data were subjected to analysis of variance, analysis of genetic parameters and multivariate analysis of principal components. Genetic variability was observed among the common bean lines, and two groups of lines with superior physiological performance according to the germination and seed vigor tests were identified. First germination count, germination, seedling length in the field, accelerated aging and emergence speed index tests are the most promising for characterizing the physiological potential of seeds of common bean lines.
Food product preparation from the vinification of 'BRS Violeta' by-products
Authors: Nogueira Tuany Yuri Kuboyama , Shimizu-Marin Victoria Diniz , Gonçales Ana Carolina , Prado Heloíza Ferreira Alves do , Silva Roberto da , Lago-Vanzela Ellen Silva
Published: October 2023

Abstract The objective of this work was to manufacture pizza dough, truffle, and cake using the paste of a by-product from the vinification of 'BRS Violeta' grape, as well as to determine some bromatological characteristics of these products. The by-product was pressed to remove excess must and crushed to obtain the paste. The paste, pasteurized at 80°C for 3 min and frozen at -18°C, was characterized for total phenolic compounds (TPC), total monomeric anthocyanins (TMA), and dietary fiber. The food products were characterized for the following parameters: moisture, energy value, and protein, lipid, ash, dietary fiber, total carbohydrates, and TPC contents. The contents found in the paste were: 326 mg GAE 100 g-1, for TPC; 185 mg mv35diglc 100g-1, for TMA; and 10 g 100 g-1, for dietary fibers. Based on the centesimal compositions of the food products, the pizza dough and truffle can be considered as a source of fiber and protein, respectively. The products show TPC contents between 51 and 63 mg GAE 100 g-1. It is possible to prepare food products of good nutritional quality, using grape by-product paste, to minimize waste in the field.
OPTIMIZATION OF FINNED HEAT SINKS WITH ELECTROSPRAY COOLING: FULL FACTORIAL METHOD
Authors: Kabakuş Abdüssamed , Yakut Kenan
Published: October 2023

In this study, the optimum heat sink geometry was determined by the full factorial method with electrospray cooling, which has started to find a place among the methods used for the removal of high heat flux in recent years. The experiments were carried out at 4.4 kV voltage, 0.2 mL/min flow rate, di= 0.61 mm nozzle diameter, and 20 mm nozzle-to-heat sink distance. Ethanol was used as the cooling fluid. The experiments were carried out in the cone jet electrospray mode. As a result of the experiments performed with square, rectangular, and triangular fin heat sinks, it was determined that the best cooling performance was achieved in the square fin heat sink. As a result of the experiments carried out with the square fin heat sink with 27 different geometries, it was determined that the most effective cooling was achieved in the Square-18 heat sink with 3 mm fin width, 3 mm fin distance, and 3 mm fin height. An average temperature value of 51°C and a fin enhancement ratio of 1.11 were obtained for eight different heat fluxes (3.2-2.99-2.75-2.59-2.4-2.19-2.02-1.88 kW/m2) in the Square-18 heat sink.
OVERVIEW OF ADDITIVE MANUFACTURING AND QUALITY ASSURANCE OF POWDERS FOR SPACE PROPULSIVE COMPONENTS
Authors: Zuin Davide , La Luna Simone , Maggi Filippo
Published: October 2023

Additive manufacturing techniques are modifying the space propulsion manufacturing panorama. These technologies, which are already implemented in manufacturing of aerospace components, are still to be further analyzed and a complete quality assurance program to be defined. In this article, a state of the art analysis of quality assurance for space propulsive applications is described. In particular, the focus is centered on material challenges associated to space propulsion: the research is oriented towards metal powders and samples implemented in selective laser melting/powder bed fusion technologies. In particular, a qualification process of the supply chain related to metal powders in space propulsion is reported. Furthermore, the application of this process to a case study material is detailed, and preliminary result data are considered. Finally, a review of the qualification process is performed and suggestions for future work on this topic are presented. This paper is published with the permission of the authors granted to 3AF - Association Aeronautique et Astronautique de France (www.3AF.fr) organizer of the Space Propulsion International conference.
Lingual myoclonus associated with brain tumour: an epileptic origin?
Authors: Yilmaz Rezzak , Arica Busra S. , Yigit Aytac
Published: October 2023

We describe a 59‐year‐old man, suffering from a left‐sided weakness, who was diagnosed with a right frontal oligodendroglioma. One month after a craniotomy, he complained of perioral numbness and slurred speech, which lasted about an hour. Neurological examination discovered dysarthric speech and repetitive, rhythmic myoclonic jerks of the tongue and the lower lip. We suggest this lingual myoclonus was an epileptic phenomenon associated with brain tumour. [Published with video sequences]
Accentuated cortico‐cortical evoked potentials in neocortical epilepsy in areas of ictal onset
Authors: Iwasaki Masaki , Enatsu Rei , Matsumoto Riki , Novak Eric , Thankappen Baburaj , Piao Zhe , O'Connor R Tim , Horning Karl , Bingaman William , Nair Dileep
Published: October 2023

Objective

To determine whether patients with neocortical epilepsy show evidence for increased excitability measured by cortico‐cortical evoked potentials (CCEPs) in ictal‐onset regions.

Methods

In patients undergoing intracranial recordings with subdural electrodes for epilepsy surgery, we measured amplitudes, latencies, and stimulus thresholds of CCEPs near ictal onset zones (iCCEPs), and compared with adjacent neocortex not associated with ictal EEG (nCCEP). CCEP amplitude and latency measurements were made with each stimulation site, using graded stimulation intensities.

Results

Ten patients were included in this study. CCEPs were recorded in eight of 10 patients. The first negative (N1) iCCEP amplitude was higher than that of nCCEP in seven of the eight patients. In the group analysis, this difference was statistically significant. In three of these patients, the difference was individually significant. In one patient, the amplitude was higher in nCCEP than iCCEP and the area selected as nCCEP was within primary eloquent cortex. There was no significant difference seen in latency changes or stimulus threshold.

Conclusions

Accentuated CCEP amplitudes near ictal onset zones could reflect an increased excitability of the cortex associated with the epileptogenic zone in some patients with neocortical epilepsy. The response of the neocortex to low‐frequency stimulation may vary depending on the presence or absence of intrinsic epileptogenicity.
Subclinical rhythmic electrographic discharges of adults and transient global amnesia: a causal or casual association?
Authors: Brigo Francesco , Bongiovanni Luigi Giuseppe , Fiaschi Antonio
Published: October 2023

Subclinical rhythmic electrographic discharges of adults (SREDA) is an uncommon distinctive EEG rhythmic pattern with uncertain significance. We report a patient with transient global amnesia in whom an EEG recording, performed after the acute phase of the neurological event, showed SREDA. Based on this EEG finding, we discuss about a possible correlation between SREDA and transient global amnesia. The presence of SREDA in our patient with transient global amnesia seems to be incidental. When described in association with transient global amnesia, SREDA should be recognized in order to avoid misdiagnosis. Although SREDA has been fortuitously described in association with transient global amnesia, a patho‐physiological correlation with mechanisms which produce it seems unlikely.
A case of levetiracetam‐induced thrombocytopenia
Authors: Oghlakian Roger , Nock Charles , Koubeissi Mohamad
Published: October 2023

Rare cases of levetiracetam‐induced thrombocytopenia have been reported in the literature. We report a case of glioblastoma multiforme and partial epilepsy treated with levetiracetam with subsequent development of thrombocytopenia. After ruling out all other possible causes of a decreased platelet count, we conclude that levetiracetam was the cause of this adverse event.
Diatom-based paleoenvironmental reconstruction in Lake Balaton, Hungary (Tó34f)
Authors: Binti Mahadi Izzati Hanun , Báldi Katalin , Korponai János , Buczkó Krisztina
Published: October 2023

A high-resolution diatom assemblage’s database was built on a 65 cm long sediment core (Tó34f), obtained from the Siófok Basin of Lake Balaton in 2017. Light microscope photo-documentation was developed out of 15,797 diatom valves. The pictures were arranged according to taxonomy from centric diatoms via araphids, monoraphids to biraphid forms. 30 categories were established to group the diatom taxa instead of species level identification requiring high level of expertise. Some (1) characteristic, and easily identifiable taxa (Karayevia clevei, Cavinula scutelloides) were distinguished on species level, (2) taxa were grouped at genus level (like Amphora, Aulacoseira, Neidium), while (3) were joined based on their morphological features. Two large groups were created based on this approach, the “light fragilarioids” and the “heavy fragilarioids”. Both groups contain diatoms with taxonomic problems, e.g. Staurosira, Staurosirella genera and other fragilarioids. This approach allowed us to make paleo-ecological reconstruction on (1) rough taxonomic data and (2) apply trait-based methods. Diatom assemblage’s zones were defined by CONISS clustering method, validated by the broken stick model. Though the planktonic trait (referring the high lake level) is insignificant (< 5%) in most of the studied record, but some peaks (>35%) point to high water level. The most recent trend of increasing number of planktonic diatoms is hypothesized to be due to the recent global warming. We demonstrated that traits are a good alternative to time and expertise demanding species-level determination of diatoms, thus a quick method is found for paleo-ecological reconstructions, while the photo-base good for detailed taxonomic work at a future time.
Improving the habits of mind of senior high school students toward biology learning through creative problem-solving learning model based on mind mapping: Pre-experimental study
Authors: Haka Nukhbatul Bidayati , Nisa Khairun , Masya Hardiyansyah
Published: October 2023

There have been many attempts to improve the habits of mind of high school students in Biology through various learning models. However, only a few research results show efforts to improve students' habits of mind through the creative problem solving learning model with mind mapping techniques. Therefore, the purpose of the study was to determine the effect of the creative problem solving learning model with mind mapping techniques on increasing the habits of mind of high school students in learning biology. The pre-experimental method was used with the one group pretest-posttest design. Involving three research classes with 108 students taken by cluster random sampling and getting the same treatment using the creative problem solving learning model with mind mapping techniques. Research data collection uses essay and habits of mind questionnaires. The implication of this research is to increase students' habits of mind. students become accustomed to using their minds critically, creatively and able to control themselves very well as evidenced by an increase in posttest scores on essay tests and habits of mind questionnaires with an average of 86% and 83% (very good) with an average N-Gain 66% and 52% (medium), so the creative problem solving learning model was recommended to increase the habits of mind of high school students in biology learning.
Learning the circulatory system through the learning management system-Moodle: How to develop teaching materials oriented towards scientific literacy?
Authors: Rusdi Rusdi , Fitria Anisa , Ristanto ‪Rizhal Hendi
Published: October 2023

Teaching materials are one of the important factors that determine the success of the learning process. The research objective was to develop a circulation system teaching material based on Moodle's LMS which is oriented towards increasing students' scientific literacy. The development model used was Thiagarajan 4D (Define, Design, Develop, and Disseminate), to the stage of feasibility testing (develop). The subjects in this study were validators of media experts, material experts, language experts, teachers and students. The data collection technique was carried out using a questionnaire to measure the validity of the open material. The results showed that the circulation system teaching materials based on the LMS-Moodle, which were oriented towards increasing scientific literacy, had an average score of 3.50 (very valid) from a maximum value of 4.00 from all aspects. Based on the results of the research, it is shown that the teaching materials of the circulation system based on the Moodle LMS which were developed to increase scientific literacy are very valid to be used as learning media for the circulation system in schools and are expected to improve students' scientific literacy.
Biology learning model to improve critical thinking skills of ten grade students: A meta-analysis
Authors: Fuadiyah Ma'lumatul , Norra Bunga Ihda , Astutik Fuji
Published: October 2023

Critical thinking skill is one of the higher-order thinking skills which has to be mastered by students. However, many studies showed that students' critical thinking skills are still relatively low. So, it is necessary to carry out learning activities that can increase critical thinking skills, one of that is through the application of learning models. The purpose of this study was to find out the types of biology learning model that can improve the critical thinking skills of class X students, the biology learning model that was most recommended to improve the biological critical thinking skills of class X students in terms of effect size value, and biology learning model that was most recommended to improve students' critical thinking skills in each material content of class X in terms of the effect size value. The type of research used was quantitative research with the research method used in the form of meta-analysis. The results of the study revealed that there were 17 types of learning models that were proven to be able to improve the biological critical thinking skills of class X students. The guided discovery learning model based on e-learning was proven to have the highest effect size value of 1,07. The learning model which was recommended to improve the critical thinking skills based on material includes: problem-based learning for biodiversity material, think pair share (TPS) cooperative model for environmental pollution material, problem-based learning combined with student facilitator and explaining for fungi, and guided discovery and e-learning based learning for ecosystem materials.
The effect of using teacher feedback on concept mastery and students’ self-assessment of the essay test on monohybrid and dihybrid cross topic
Authors: Hasyaty Allyany , Sriyati Siti , Amprasto Amprasto
Published: October 2023

This study aims to identify the effect of using teacher feedback on students' concept mastery and self-assessment on the topic of monohybrid and dihybrid crosses. The method used in this research was a quantitative quasi-experiment with Nonequivalent Control Group research design. The participants in this study were among 69 ninth grade students (33 control class students and 36 experimental class students) at one of the public junior high schools in Bandung Regency. This study uses positive written feedback with the types of supporting, direct corrective and guidance on students' essay test answer sheets. To measure students' mastery of concepts, an essay test was used with a total of 10 questions. To measure students' self-assessment, a 1-4 Likert scale questionnaire was used using indicators that were adjusted to the indicators on the essay questions. The results showed that there is a significant difference in students' concept mastery and students' self-assessment ability in the control class and experimental class. As for the N-Gain test, the category of concept mastery improvement in the control class belongs to the low criteria (0.19) and the experimental class belongs to the medium criteria (0.57). Students' self-assessment skills in the control class (60%) and experimental class (70%) are classified in the sufficient and good categories. Students' responses to the teacher’s feedback showed a positive response. Based on this research, the use of teacher feedback on essay tests affects students' concept mastery and self-assessment.
Validity test of "Sigeram" as an android-based biology learning interactive media on human movement system topic
Authors: Hapsari Putri , Utami Nurul Hidayati , Noorhidayati Noorhidayati
Published: October 2023

This study aims to describe the suitability of "Sigeram" derived from the acronym "sistem gerak manusia” or human movement system to become an android-based interactive learning media for biology subjects. This study uses descriptive research with four stages, namely analysis, design, development, and validation. The data collection technique used a validity questionnaire. The validity test subjects consisted of three practitioners, including a teacher from Senior Hugh School 12 Banjarmasin and two lecturers from the study program of Biology Education, Universitas Lambung Mangkurat. “Sigeram” application consists of some menus, including learning prerequisites, identity, learning implementation components, meeting schedule, concept map, then the material section, summary, glossary, quiz, bibliography, and profile. Additional features in the interactive media are the "Bio Info", "Let's Find Out", "Let's Discuss", "Act Creatively", "Habituation", "Reflection", and "Final Project". Testing the validity of "Sigeram" as an interactive media for learning Biology based on android showed a validity score of 4.29 with a very valid category, so that "Sigeram" can be used as an interactive media for learning biology, especially in human movement system topic.
Features of regulation of hepcidin and ferroportin in cancer patients (literary review)
Authors: Blindar V. N. , Zubrikhina G. N. , Davydova T. V. , Dobrovolskaya M. M.
Published: October 2023

Background. The pathways of iron acquisition, outflow, storage and regulation are disrupted in cancer, which suggests that the reprogramming of iron metabolism is one of the central aspects of the survival of tumor cells.

Aim. Is to review and generalize modern literature data on the regulation of hepcidin, ferroportin and prospects for the correction of iron metabolism in cancer patients.

Materials and Methods. The paper presents the results of international and domestic studies of the peculiarities of iron metabolism and the prospects for its correction in cancer patients. The search for relevant sources was carried out in the web of Science, PubMed, Medline, eLibrary.ru systems for 1988–2023. Of the analyzed studies 61, the most relevant, were used to write a systematic review.

Results. Over the past decade, a new understanding has emerged of the role of proteins, in particular hepcidin and ferroportin, which regulate cellular iron in cancer growth, angiogenesis and metastasis. New treatment methods with hepcidin-modifying strategies and stabilizers of hypoxia-induced factors are emerging, but their therapeutic efficacy for correcting iron metabolism in cancer patients needs to be evaluated and clinical trials.

Conclusion. Analysis of the literature data has shown the high relevance of studies of the regulation of hepcidin and ferroportin in cancer patients and the need for further study of this problem.
Schisandrin and Schisantherin A in Multiphytoadaptogen for Preventive Oncology
Authors: Bocharova O. A. , Kazeev I. V. , Shevchenko V. E. , Ionov N. S. , Sheichenko O. P. , Bocharov E. V. , Karpova R. V. , Kucheryanu V. G. , Lagunin A. A. , Filimonov D. A. , Kosorukov V. S. , Poroikov V. V. , Tutelyan V. A. , Pyatigorskaya N. V.
Published: October 2023

Background. The original herbal formula of Multiphytoadaptogen (MPhA) for preventive oncology developed by the N. N. Blokhin Center of Oncology containing phytocomponents from Schisandra chinensis (Turcz.) Baill (Schisandraceae), has been investigated in vitro, in vivo. Preliminary efficiency in clinical trials has also been obtained. This was allowed because MPhA in Russia is registered as a parapharmaceutical agent and therefore standardized according to established requirements. However, due to the high efficiency of MPhA, a detailed study of the chemical composition and standardization of it is required, including the Schisandra chinensis active components, which turned out to be translocated into MPhA as a result of the extraction technology developed.

The aim of the study was to identify the Schisandra chinensis biologically active substances in MPhA and to evaluate the biological activity profiles of the identified phytocomponents using in silico analysis.

Materials and methods. we used high performance liquid chromatography in combination with mass spectrometry (HPLC–MS / MS). Chromatography was performed on an ACQUITY UPLC BEH C18 column in a gradient mode. A TSQ Vantage triple quadrupole mass spectrometer with electrospray ionization was used. we performed in silico analyzes of Schisandrin and Schisantherin A biological activity spectra using computer programs PASS and PharmaExpert.

Result. The secondary metabolites lignans Schisandrin and Schisantherin A were identified in the herbal formula MPhA. Schisandrin and Schisantherin A activities, according the scientific literature and in silico analysis, correspond to the properties studied for MPhA which therefore fits into the concept of medication for preventive oncology.

Conclusion. The determined secondary metabolites can be used for identification, standardization and quality testing of the herbal formula MPhA.