bluuebunny/crossref_metadata_2025 huggingface dataset:
From Omics to Multi-Omics: A Review of Advantages and Tradeoffs
Authors: Hayes C. Nelson , Nakahara Hikaru , Ono Atsushi , Tsuge Masataka , Oka Shiro
Published: November 2024

Bioinformatics is a rapidly evolving field charged with cataloging, disseminating, and analyzing biological data. Bioinformatics started with genomics, but while genomics focuses more narrowly on the genes comprising a genome, bioinformatics now encompasses a much broader range of omics technologies. Overcoming barriers of scale and effort that plagued earlier sequencing methods, bioinformatics adopted an ambitious strategy involving high-throughput and highly automated assays. However, as the list of omics technologies continues to grow, the field of bioinformatics has changed in two fundamental ways. Despite enormous success in expanding our understanding of the biological world, the failure of bulk methods to account for biologically important variability among cells of the same or different type has led to a major shift toward single-cell and spatially resolved omics methods, which attempt to disentangle the conflicting signals contained in heterogeneous samples by examining individual cells or cell clusters. The second major shift has been the attempt to integrate two or more different classes of omics data in a single multimodal analysis to identify patterns that bridge biological layers. For example, unraveling the cause of disease may reveal a metabolite deficiency caused by the failure of an enzyme to be phosphorylated because a gene is not expressed due to aberrant methylation as a result of a rare germline variant. Conclusions: There is a fine line between superficial understanding and analysis paralysis, but like a detective novel, multi-omics increasingly provides the clues we need, if only we are able to see them.
In Silico Hydrolysis of Lupin (Lupinus angustifolius L.) Conglutins with Plant Proteases Releases Antihypertensive and Antidiabetic Peptides That Are Bioavailable, Non-Toxic, and Gastrointestinal Digestion Stable
Authors: Arámburo-Gálvez Jesús Gilberto , Tinoco-Narez-Gil Raúl , Mora-Melgem José Antonio , Sánchez-Cárdenas Cesar Antonio , Gracia-Valenzuela Martina Hilda , Flores-Mendoza Lilian Karem , Figueroa-Salcido Oscar Gerardo , Ontiveros Noé
Published: November 2024

Lupin (Lupinus angustifolius L.) proteins are potential sources of bioactive peptides (LBPs) that can inhibit dipeptidyl peptidase IV (DPP-IV) and angiotensin I-converting enzyme (ACE-I) activity. However, the capacity of different enzymes to release LBPs, the pharmacokinetic and bioactivities of the peptides released, and their binding affinities with the active sites of DPP-IV and ECA-I are topics scarcely addressed. Therefore, we used in silico hydrolysis (BIOPEP-UWM platform) with various enzymes to predict the release of LBPs. Among the bioactive peptides identified in lupin proteins (n = 4813), 2062 and 1558 had DPP-IV and ACE-I inhibitory activity, respectively. Ficin, bromelain, and papain released the highest proportion of ACE-I (n = 433, 411, and 379, respectively) and DPP-IV (n = 556, 544, and 596, respectively) inhibitory peptides. LBPs with favorable pharmacokinetics and gastrointestinal stability tightly interacted with the active sites of ACE-I (–5.6 to –8.9 kcal/mol) and DPP-IV (–5.4 to –7.6 kcal/mol). Papain generated the most bioavailable LBPs (n = 459) with ACE-I (n = 223) and DPP-IV (n = 412) inhibitory activity. These peptides were non-toxic and gastrointestinal digestion stable. Notably, papain-based hydrolysis released some LBPs (n = 270) that inhibited both ACE-I and DPP-IV. Plant protease-based hydrolysis is a promising approach for producing lupin hydrolysates with ACE-I and DPP-IV inhibitory activities.
Outcomes and Prognosis of COVID-19-Induced Adult Respiratory Distress Syndrome Patients Treated with Prolonged Veno-Venous Extracorporeal Membrane Oxygenation: A Retrospective Multicenter Study
Authors: Bitan Amram , Sagie Nitzan , Ilgiyaev Eduard , Stavi Dekel , Makhoul Maged , Soroksky Arie , Kasif Yigal , Novack Victor , Galante Ori
Published: November 2024

Background: Predicting whether extracorporeal membrane oxygenation (ECMO) treatment duration affects prognosis is important both medically and economically. Methods: We conducted a retrospective, multicenter study to better understand the outcomes of patients treated with veno-venous (VV) ECMO over a prolonged duration, analyzing data from the Israel ECMO registry. The study included all adult patients treated with VV-ECMO due to COVID-19-induced respiratory failure. The primary outcomes were survival rates up to 180 days from cannulation. Results: One hundred and eighty-eight patients were included in the study. The median age was 50 years (IQR 42, 50), and 69% were male. Patients were mechanically ventilated for a median of 2.5 days before cannulation (IQR 0.5, 5). The mean ECMO support duration was 29.9 days, with a maximal duration of 189.9 days. The survival rate for 180 days was 56%. We found no change in survival for patients on ECMO for 14, 28, or 56 days. Every day of mechanical ventilation before cannulation correlated with an 11% greater risk for prolonged ECMO treatment (p = 0.01). Conclusions: COVID-19-induced ARDS patients treated with VV-ECMO for prolonged duration had the same prognosis as those treated for short periods of time. The longer the duration of mechanical ventilation before ECMO cannulation, the higher the risk for prolonged ECMO treatment.
Open-Access 12-Minute MRI Screening for Acute Appendicitis: A Five-Year Retrospective Observational Study of Diagnostic Accuracy
Authors: Jones Andrew Owen , Nol James
Published: November 2024

Objective: This retrospective observational diagnostic accuracy study aims to demonstrate that open-access rapid-sequence non-contrast magnetic resonance imaging (MRI) is accurate for exclusion or confirmation of acute appendicitis (AA). Methods: In 2017, a locally designed 12 min MRI protocol was made available as a new open-access option (no booking needed) for any emergency department (ED) or acute surgical patient with any clinical presentation at the authors’ sites. Uninterrupted single-radiologist reporting availability was provided. A 5-year consecutive report list from 1 January 2019 to 31 December 2023 was recorded in an activity log, from which 3478 eligible reports were identified as ED-based referrals assessing for possible AA. There was “appendicitis possibility” in 581/3478 (17%) reports and “no evidence of appendicitis” in 2897/3478 (83%). These were retrospectively compared with the medical record findings of 557/3478 proven cases of AA (16%). Report availability and reliability metrics have been assessed. Results: Overall, 2583/3478 reports (74%) were finalized within 2 h of MRI study completion, 3254/3478 (94%) within 4 h. The 3478 reports combined had 98% sensitivity, 98% specificity, 98% accuracy, 94% positive predictive value, and 99% negative predictive value for AA (φ = 0.95). The largest 5-year subgroup, females 15–19 years old, 66/440 with proven AA, had 98% report accuracy. Pregnant women, 21/171 with proven AA, had 99% report accuracy. Conclusions: The described MRI protocol is accurate for appendicitis assessment and is a suitable first-imaging choice for children, young adults, and pregnant women. It does not require intravenous contrast and poses no radiation risk.
Correlation Between the Pitch and Loudness of Tinnitus, Hearing Levels, and Tinnitus Handicap Inventory Scores in Patients with Chronic Subjective Tinnitus
Authors: Mavrogeni Panayiota , Molnár András , Molnár Viktória , Tamás László , Maihoub Stefani
Published: November 2024

Objectives: The aim of this study was to investigate the relationships between tinnitus pitch and loudness, the frequency of hearing loss, hearing level, and the Tinnitus Handicap Inventory (THI) scores reported by patients. Methods: In total, 139 patients (50 men and 89 women; mean age ± SD, 60.19 ± 11.47 years) who suffered from chronic primary tinnitus associated with sensorineural hearing loss were included in the study. Participants underwent pure-tone audiometry and tinnitus pitch matching assessments. Additionally, all participants completed the Hungarian version of the THI questionnaire. Correlations were assessed using simple linear, Spearman’s, and Pearson’s correlation tests, and a linear regression model was applied. The Mann–Whitney U test was also used. Results: This study identified a significant correlation between the pitch of tinnitus and the frequency of hearing loss (p = 0.000 *; rho = 0.549). There was also a significant correlation between tinnitus noise and hearing levels (p = 0.000 *; rho = 0.375). Age was shown to significantly affect tinnitus loudness (p = 0.016 *) and hearing levels (p = 0.000 *) as determined by a linear regression model. Tinnitus duration only significantly influenced tinnitus loudness (p = 0.022). There was no significant effect of sex on tinnitus or audiometry parameters. Total THI scores were influenced solely by tinnitus loudness (p = 0.021 *). Furthermore, sex did have an effect on total THI scores, with women reporting higher scores (p = 0.000 *). Conclusions: This study concluded that there is a significant correlation between the pitch and loudness of tinnitus and hearing levels, suggesting a connection in their underlying mechanisms. The intensity of tinnitus and hearing level are primarily affected by ageing processes. Furthermore, the severity of self-perceived tinnitus is mainly related to the loudness of tinnitus.
From Crisis to Complications: A Nationwide Cohort Study Assessing One-Year Cardiovascular and Thromboembolic Risks After Severe COVID-19 Compared to Matched Controls
Authors: Liliequist Andreas , Svensson Per , Hofmann Robin , Häbel Henrike , Ståhlberg Marcus , Nordberg Per
Published: November 2024

Background: The long-term risk of cardiovascular and thrombotic events following severe COVID-19 remains largely unknown. This study aimed to assess the risk of atherosclerotic cardiovascular disease (ASCVD) within one year after hospital discharge in patients who received intensive care for severe COVID-19. Methods: A register-based nationwide case-control study on a cohort of patients with severe COVID-19 (cases) requiring mechanical ventilation and discharged alive without experiencing cardiovascular or thrombotic events during their hospital stay. Each case was matched (age, sex, district of residence) with up to 10 population-based controls. The primary outcome was ASCVD occurring after hospital discharge, defined as a composite endpoint, including myocardial infarction (MI), unstable angina pectoris and ischemic stroke. Secondary endpoints were MI, stroke, all-cause mortality, and venous thromboembolic events. Hazard ratio (HR) (95% CI) was used with adjustments for age, sex, socioeconomic factors, and co-morbidities. Results: In total, 31,375 individuals (70% men, median age 62 years) were included, of which 2854 had severe COVID-19 and 26,885 matched control subjects. The adjusted HR for ASCVD during the first year compared to control subjects was 3.1 (95% CI 1.7–5.4). Adjusted HRs for secondary outcomes for myocardial infarction were 2.0 (95% CI 0.8–5.3), for stroke 1.9 (95% CI 0.7–5.3), for pulmonary embolism 49.4 (95% CI 28.0–87.1), and deep venous thrombosis (DVT) 16.0 (95% CI 7.8–32.6). Conclusions: Severe COVID-19 requiring intensive care was associated with a substantial increase in 1-year risk for ASCVD and venous thromboembolic events.
Is the Anamnesis Enough to De-Label Patients with Reported Beta-Lactam Allergy?
Authors: Rozłucka Lesia , Rymarczyk Barbara , Gawlik Radosław , Glück Joanna
Published: November 2024

Background: The decision whether to de-label patient with suspected BL hypersensitivity is based on risk stratification. The aim of this study was to prepare a characteristic of diagnostic risk groups and to create a model enabling the identification of the low-risk diagnostic group. Methods: We analyzed the medical records of patients hospitalized due to suspected hypersensitivity to BL antibiotics. Based on their medical-history data, patients were divided into three diagnostic risk groups, using the criteria proposed by Shenoy et al. Univariate and multivariate analysis models were used to create a diagnostic tool. Results: Among 263 patients referred for BL hypersensitivity diagnosis, 88 (33.5%) were allocated to group I, 129 (49%) to group II, and 46 (17.5%) to group III. There were significant differences between diagnostic risk groups regarding history of hypersensitivity to penicillins (p < 0.001), cephalosporins (p < 0.001), >1 BL (p < 0.05), several episodes of BL hypersensitivity (p < 0.001), medical intervention (p < 0.001), documented hypersensitivity (p < 0.001), time from drug intake to symptoms (p < 0.001), and time from hypersensitivity to diagnosis (p < 0.001). In total, 81 patients (30.8%) were de-labeled: 52 (59.8%) in group I, 27 (20.9%) in group II, and 2 (4.3%) in group III. The univariate analysis model of the low-diagnostic-risk group applied to the de-labeled part showed 90% specificity and 21.93% sensitivity. NPV and PPV were estimated at 72.04% and 49.53%, respectively. The multivariate model had high specificity but low sensitivity; its NPV was 76%, with 68% PPV. Conclusions: The tool enabling the identification of low-diagnostic-risk patients based on anamnesis is not sensitive enough to de-label patients on its basis.
Correction: Gao et al. High Expression of PDK4 Could Play a Potentially Protective Role by Attenuating Oxidative Stress after Subarachnoid Hemorrhage. J. Clin. Med. 2022, 11, 3974
Authors: Gao Xuan , Gao Yong-Yue , Wu Ling-Yun , Peng Zheng , Liu Xun-Zhi , Chen Xiang-Xin , Gao Sen , Zhang Hua-Sheng , Lu Yue , Hang Chun-Hua , Zhuang Zong , Li Wei
Published: November 2024

In the original publication [...]
Endothelial Glycocalyx Anomalies and Ocular Manifestations in Patients with Post-Acute COVID-19
Authors: Azar Georges , Abdelmassih Youssef , Bonnin Sophie , Guindolet Damien , Vasseur Vivien , Behar Cohen Francine , Salmon Dominique , Mauget-Faÿsse Martine
Published: November 2024

Objectives: To report ophthalmological and microvascular findings in patients with post-acute COVID-19. Methods: In this prospective, monocentric cohort study, we included patients with post-acute COVID-19 who presented with ophthalmological symptoms. All patients underwent indocyanine green angiography (ICGA), OCT, OCT-angiography, adaptive optics, and GlycoCheck assessments. Results: We included 44 patients, predominantly female (81.8%), with a mean age of 47.5 ± 11.5 years. Key ICGA findings revealed hyperreflective dots in 32 eyes (36.4%) and hemangioma-like lesions in 7 eyes (8.0%). Capillary non-perfusion in the superficial capillary plexus (SCP) and deep capillary plexus (DCP) was observed in 42 eyes (47.7%) and 21 eyes (23.9%), respectively. Eyes with hyperreflective dots exhibited a lower perfused boundary region (PBR), while those with superficial punctate keratitis showed a higher PBR (p = 0.02 and p = 0.002, respectively). Eyes with capillary non-perfusion in the SCP displayed lower capillary densities (CD4, CD5, and CD4-6; p = 0.001, 0.03, and 0.03, respectively), and eyes with non-perfusion in the DCP had lower CD4 (p = 0.03). A negative correlation was identified between capillary density and the wall-to-lumen ratio. Conclusions: Patients with post-acute COVID-19 demonstrate both retinal and choroidal vascular anomalies. Ocular pathology was associated with reduced capillary density. These injuries appear to stem more from microvascular disruptions than from persistent glycocalyx abnormalities.
Impacts of Climate Change and Land Use/Cover Change on Runoff in the Huangfuchuan River Basin
Authors: Huang Xin , Qiu Lin
Published: November 2024

Studying the response of runoff to climate change and land use/cover change has guiding significance for watershed land planning, water resource planning, and ecological environment protection. Especially in the Yellow River Basin, which has a variable climate and fragile ecology, such research is more important. This article takes the Huangfuchuan River Basin (HFCRB) in the middle reaches of the Yellow River as the research area, and analyzes the impact of climate change scenarios and land use/cover change scenarios on runoff by constructing a SWAT model. Using CMIP6 GCMs to obtain future climate data and the CA–Markov model to predict future land use data, the two are coupled to estimate the future runoff process in the HFCRB, and the uncertainty of the estimated runoff is decomposed and quantified. The results were as follows: ① The SWAT model has good adaptability in the HFCRB. During the calibrated period and the validation period, R2 ≥ 0.84, NSE ≥ 0.8, and |PBIAS| ≤ 17.5%, all of which meet the model evaluation criteria. ② There is a negative correlation between temperature and runoff, and a positive correlation between precipitation and runoff. Runoff is more sensitive to temperature rise and precipitation increase. ③ The impact of land use types on runoff is in the order of cultivated land > grassland > forest land. ④ The variation range of runoff under the combined effects of future climate change and LUCC is between that of single climate change or LUCC scenarios. The increase in runoff under SSP126, SSP245, and SSP585 scenarios is 10.57%, 25.55%, and 31.28%, respectively. Precipitation is the main factor affecting the future runoff changes in the HFCRB. Model uncertainty is the main source of uncertainty in runoff prediction.
Spinal Regulation of Posture: Effects of Transcutaneous Spinal Cord and Affective Sound Stimulation
Authors: Shamantseva Natalia , Semenova Varvara , Timofeeva Olga , Andreeva Irina , Moshonkina Tatiana
Published: November 2024

The combined effects of transcutaneous electrical stimulation (tES) of the spinal cord and affective sound stimulation on postural control were investigated to elucidate the involvement of spinal networks in the maintenance of vertical stability. Healthy volunteers (n = 27) underwent tES and sound stimulation separately and combined quasi-randomly. All participants were field-dependent; i.e., participants used the exteroceptive afferent stream for spatial orientation. Centre-of-pressure parameters were analysed to assess postural stability. Results showed that tES at the T11–T12 vertebrae stabilised posture, tES at the L1–L2 vertebrae had no postural effect, and sound stimulation from the left destabilised posture. To assess the role of spinal regulation of postural disturbances, we compared the effects of combined tES with sound stimulation to those of sound stimulation alone. Stimulation at the T11–T12 level reduced the lateral sway induced by affective sounds, whereas L1–L2 tES did not. These results suggest that, in healthy individuals, spinal networks located at the T11–T12 and L1–L2 vertebral levels have distinct roles in maintaining upright posture, both when a person is standing still and when they are actively stabilising their posture during destabilising perturbations. T11–T12 spinal networks stabilise upright posture when destabilising information is solely transmitted from the supraspinal level.
Spark Plasma Sintering Preparation of Tungsten Carbide-Reinforced Iron-Based Composite Materials: Wear Resistance Performance and Mechanism
Authors: Zeng Xiaoyi , Wang Renquan , Liu Ying
Published: November 2024

The spark plasma sintering (SPS) process was used to create iron-based composites reinforced with tungsten carbide (WC) particles of various morphologies, and the effect of WC particle morphology on material wear resistance was systematically investigated. The experiment revealed that the addition of non-spherical WC (CTC-A) significantly altered the composites’ friction coefficient, wear morphology, and wear mechanism. As the CTC-A content increased, the composites’ wear rate decreased at first, then increased, and then decreased again. Composites with a CTC-A concentration of 10% had a minimum wear rate of 2.7 × 10−6 mm3/(N·m) and peaked at 20%. SEM analysis indicated that the wear mechanism gradually changed from initial oxidative wear to abrasive wear as the CTC-A content increased, and the wear morphology transitioned from smooth to rough with the appearance of numerous abrasives and cracks. The study demonstrated that the low content of non-spherical WC particles during sintering significantly increased the hardness of the matrix by forming carbide phases, while a high content led to increased surface roughness, inducing abrasive wear and reducing wear performance. These findings provide a significant theoretical basis and practical guidance for optimizing the design of iron-based composites.
Analysis of Static and Cyclic Properties of 316L and AlSi10Mg in Conventional Casting and Additive Manufacturing
Authors: Chmelko Vladimír , Margetin Matúš , Zetková Ivana , Norek Martin , Likavčan Filip
Published: November 2024

The paper presents the original results of cyclic testing of materials that are identical in chemical composition but produced by two different technologies: conventional metallurgy and additive manufacturing. For the aluminium alloy AlSi10Mg and the austenitic steel 316L, tensile curves, tension–compression and torsion alternating fatigue curves are experimentally obtained and presented. The experimental results are compared for two fabrication technologies—conventional metallurgy and additive DLMS technology. The results indicate a significant effect of anisotropy on the fatigue performance of the AM materials and a different slope of the fatigue life curves in the cyclic torsion versus cyclic tension–compression. The static and, in particular, the fatigue properties of both materials are discussed in relation to the microstructure of the materials after conventional production and after additive manufacturing. This comparison allowed us to explain both the causes of the anisotropy of the AM materials and the different slope of the curves for normal and shear stresses under cyclic loading. Using the example of the strength assessment of bicycle frames, the possibility of progressively wider use of additive manufacturing for load-bearing structures is presented.
Aluminum-Free Borosilicate Glass Functionalized Hydrogels for Enhanced Dental Tissue Regeneration
Authors: Attik Nina , Basri Inès , Sohier Jérôme , Gauthier Rémy , Villat Cyril , Goutaudier Christelle
Published: November 2024

Hydrogels are promising scaffolds for tissue regeneration, and borosilicate glass particles have demonstrated potential in enhancing the biological behaviour of dental pulp cells. However, the specific morphological characteristics of dental lesions and the diverse requirements of dental tissues require biocompatible, bioactive, and shapeable scaffolds. This study aimed to evaluate the in vitro biological behaviour of human gingival fibroblasts (HGFs) in contact with an experimental aluminum-free borosilicate glass-functionalized hydrogel. Two types of experimental borosilicate glass particles were utilized, with Biodentine® particles serving as a reference material. The hydrogel, based on poly(L-lysine) dendrimers (DGL) with or without borosilicate particles, was analyzed using micro-computed tomography (µCT) and scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy (EDX). Cytocompatibility was assessed using Live/Dead™ staining, and cell colonization was evaluated via confocal imaging. Additionally, Alizarin red staining was performed to assess mineralization potential after 7 and 14 days. Results indicated that the incorporation of borosilicate particles did not alter hydrogel porosity, while EDX confirmed particle presence on the hydrogel surfaces. Furthermore, the borosilicate-functionalized hydrogels significantly enhanced cell proliferation, colonization, and the content of calcium deposits. These findings highlight the potential of these hydrogels for future clinical applications in dental tissue regeneration, pending further development.
Friction Performance Analysis of WC-Reinforced IN718 Composite Material Based on SLM Process
Authors: Zhao Xuejin , Zhao Xiaoyu , Xu Youfan , Shi Yongjun
Published: November 2024

To enhance the wear resistance of nickel-based high-temperature alloys, IN718/WC composites were prepared via selective laser melting (SLM). The optimal molding process parameters of IN718/WC composites were determined using a combination of experimental and simulation methods. Based on the SEM images of the composites, a gradient transition layer was found to form between the WC particles and the IN718 matrix, and the γ′ and γ″ reinforcing phases dispersed into the matrix, providing lubrication and reducing wear during friction. The influence of WC content on the wear resistance of the composites was investigated. When the WC content was 5%, the molded parts showed optimal wear resistance, the friction coefficient fluctuated steadily, the degree of wear was low, and the amount of wear was reduced to 0.02973 mm3. The average friction coefficient and wear volume of the molded parts with similar contents decreased by 26.95% and 4.27%, respectively, compared with the pure IN718-molded parts. This study provides a case study and guidance for further optimization of the molding process for nickel matrix high-temperature composites prepared using the SLM method.
Plasma-Assisted Preparation of Reduced Graphene Oxide and Its Applications in Energy Storage
Authors: Li Haiying , Han Yufei , Qiu Pengyu , Qian Yuzhe
Published: November 2024

Reduced graphene oxide (rGO) exhibits mechanical, optoelectronic, and conductive properties comparable to pristine graphene, which has led to its widespread use as a method for producing graphene-like materials in bulk. This paper reviews the characteristics of graphene oxide and the evolution of traditional reduction methods, including chemical and thermal techniques. A comparative analysis reveals that these traditional methods encounter challenges, such as toxicity and high energy consumption, while plasma reduction offers advantages like enhanced controllability, the elimination of additional reducing agents, and reduced costs. However, plasma reduction is complex and significantly influenced by process parameters. This review highlights the latest advancements in plasma technology for reducing graphene oxide, examining its effectiveness across various gas environments. Inert gas plasmas, such as argon (Ar) and helium (He), demonstrate superior reduction efficiency, while mixed gases facilitate simultaneous impurity reduction. Additionally, carbon-based gases can aid in restoring defects in graphene oxide. This paper concludes by discussing the future prospects of plasma-reduced graphene and emphasizes the importance of understanding plasma parameters to manage energy and chemical footprints for effective reduction.
Influence of Socioeconomic Status on the Retail Food Environment in Alicante
Authors: Hernández-Caravaca Iván , Martínez-García Alba , Trescastro-López Eva María , Plaza-Gavaldón Ángel , Martí-Cremades Julio , Moncho Joaquín
Published: November 2024

Background/Objectives: Unhealthy dietary habits are associated with chronic non-communicable diseases and may contribute to increased mortality in all countries of the world. Food environments determine the accessibility, availability, and promotion of food, thus playing an important role in people’s diets, but they are context-dependent. The aim of this study was to evaluate the availability and accessibility of food in food outlets in different neighborhoods of the city of Alicante. Methods: Cross-sectional study. Trained researchers conducted food store audits using the validated Nutrition Environment Measures Survey in Stores for Mediterranean contexts (NEMS-S-MED) tool. Data were collected from food stores within a socioeconomically diverse sample in Alicante (n = 63). We compared the availability and affordability of “healthier–less healthy” food pairs, scores between food store types (supermarkets, specialized, convenience stores, and others), and socioeconomic levels in Alicante in 2022. Results: The present study highlights that the food stores with the greatest availability and accessibility to healthy foods are supermarkets, as opposed to specialized stores and convenience stores. In addition, differences were found in the accessibility and availability of healthy foods by neighborhoods with different income levels, being more accessible in the residential neighborhood with the highest income level. Tourism could explain the differences in the food retail environment, with a high-income neighborhood showing similar results to low-income neighborhoods. Conclusions: The results obtained invite reflection on the development and adoption of policy strategies that promote the availability and accessibility of healthy food in the most disadvantaged areas.
The Interactions Between Diet and Gut Microbiota in Preventing Gestational Diabetes Mellitus: A Narrative Review
Authors: Beldie Luiza-Andreea , Dica Cristina-Camelia , Moța Maria , Pirvu Bianca-Florentina , Burticală Marilena-Alexandra , Mitrea Adina , Clenciu Diana , Efrem Ion Cristian , Vladu Beatrice Elena , Timofticiuc Diana Cristina Protasiewicz , Roșu Maria Magdalena , Gheonea Theodora Claudia , Amzolini Anca Maria , Moța Eugen , Vladu Ionela Mihaela
Published: November 2024

Recent studies have revealed that dysbiosis, defined as alterations in gut microbiota, plays an important role in the development and the progression of many non-communicable diseases, including metabolic disorders, such as type 2 diabetes mellitus and gestational diabetes mellitus (GDM). The high frequency of GDM makes this disorder an important public health issue, which needs to be addressed in order to reduce both the maternal and fetal complications that are frequently associated with this disease. The studies regarding the connections between gut dysbiosis and GDM are still in their early days, with new research continuously emerging. This narrative review seeks to outline the mechanisms through which a healthy diet that protects the gut microbiota is able to prevent the occurrence of GDM, thus providing medical nutritional therapeutic perspectives for the management of GDM.
The Interconnection Between Systemic Lupus Erythematosus and Diet: Unmet Needs, Available Evidence, and Guidance—A Patient-Driven, Multistep-Approach Study
Authors: Sciascia Savino , Ferrara Gabriele , Roccatello Lorenzo , Rubini Elena , Foddai Silvia Grazietta , Radin Massimo , Cecchi Irene , Rossi Daniela , Barinotti Alice , Ricceri Fulvio , Gilcrease Winston , Baldovino Simone , Ferreira Poshar Andrea , Conti Alessio , Fenoglio Roberta
Published: November 2024

Background/Objectives: Inflammation and immunological dysregulation are central to systemic lupus erythematosus (SLE), a complex autoimmune disease. Recently, there has been increasing interest in the potential role of dietary factors in SLE. This study aimed to explore the relationship between diet and SLE by addressing patient needs, conducting a systematic review, and providing guidance to the patient community. Methods: This four-step study started with a survey of patients with SLE that was conducted to gather frequently asked questions (FAQs) related to diet. Using the PICO framework, two comprehensive systematic literature searches were performed in PubMed to address these FAQs. Subsequently, the evidence retrieved was used to answer FAQs and provide guidance to people with SLE. A second survey was conducted to gather patient feedback on the topics and guidance provided. Results: A literature review of 28 systematic reviews was performed, evaluating the impact of diet on inflammation, immune response, and health outcomes in SLE patients. The review focused on key nutritional elements, including vitamin D, omega-3 fatty acids, curcumin supplements, and low-calorie or low-glycemic index diets. Seven guidance statements were developed based on these findings. All the answers provided were positively assessed by participants. Conclusions: This patient-centered study improves our understanding of the diet–SLE relationship through systematic reviews and patient feedback. While specific dietary recommendations for SLE are not yet established, patient input underscores the need for ongoing research to optimize treatment strategies and quality of life for those with SLE.
Strategies for Restructuring Dietetics Education Programs to Improve Nutrition Equity in Indigenous Populations: A Narrative Review
Authors: Klassen Pamela N. , Chan Catherine B.
Published: November 2024

Background/Objectives: Particularly in racially and ethnically diverse countries, the necessity of providing individualized care to people seeking diet advice is increasingly recognized and embedded in practice guidelines. Some jurisdictions have a history of colonization with subjugation and marginalization of the Indigenous population, which has led to serious health inequities. One overarching strategy to reduce health inequities is to provide education through a decolonizing lens, so that graduate healthcare professionals, such as dietitians, have a better understanding of how to mitigate colonial attitudes, racism, stereotyping and other behaviours, thereby improving health equity. This review aims to summarize and evaluate educational strategies to decolonize dietetics training programs. Methods: A narrative review was conducted. Results: Professional dietetics organizations in Canada, Australia and New Zealand have incorporated Indigenous-specific outcomes into their standards of practice. Six primary research studies were reviewed, two each from Australia, New Zealand and the United States. The strategies developed include reviewing curriculum content, providing experiential learning opportunities and identifying barriers to the participation of Indigenous students in dietetics programs. Lack of engagement of Indigenous persons in curriculum development, planning and evaluation of efforts is a gap that needs to be addressed. Conclusions: Meeting practice standards and closing the health equity gap for Indigenous peoples require additional research and implementation into practice.
Fabrication and Characterization of Phyllanthus Emblica Extract-Polyvinyl Alcohol/Carboxymethyl Cellulose Sodium Antioxidant Hydrogel and Its Application in Wound Healing
Authors: Huang Shanqin , Li Shanglun , Li Guoyan , Wang Chenyu , Guo Xiaohan , Zhang Jing , Liu Jing , Xu Ying , Wang Yanchun
Published: November 2024

Background: Phyllanthus emblica is a medicinal and edible plant from the Euphorbiaceae family, notable for its rich content of polyphenols and flavonoids, which provide significant antioxidant properties. To exploit the full antioxidant potential of Phyllanthus emblica, this study developed a hydrogel system incorporating polyvinyl alcohol (PVA) and carboxymethyl cellulose sodium (CMC-Na), integrated with Phyllanthus emblica extract, for the purpose of wound healing. Methods: The extraction process of active ingredients of Phyllanthus emblica was optimized and assessed the antioxidant composition and activity of the extract. A series of hydrogel performance evaluations were performed on the Phyllanthus emblica extract-loaded PVA/CMC-Na hydrogel (AEPE composite hydrogel). Additionally, the wound healing efficacy was evaluated through cell culture experiments and wound healing assays using BALB/C mice. Results: The findings indicated that the extraction of Phyllanthus emblica with 95% ethanol yielded an extract rich in polyphenols, primarily gallic acid and ellagic acid, demonstrating high free radical scavenging capacity and robust antioxidant activity. The hydrogel matrix containing 12% PVA and 1% CMC-Na exhibited excellent physicochemical properties. The optimized AEPE composite hydrogel enabled sustained drug release over a 24 h period, exhibited low cytotoxicity and promoted cell migration. In a mouse dorsal wound healing model, the AEPE composite hydrogel showed pronounced anti-inflammatory and antioxidation effects, enhanced collagen deposition, and ultimately accelerated wound healing. Conclusions: The AEPE composite hydrogel demonstrated strong antioxidant characteristics and significant wound healing potential. Thus, this study could broaden the application prospects of Phyllanthus emblica in wound healing.
The Formulation and Evaluation of Customized Prednisolone Gel Tablets Prepared by an Automated Extrusion-Based Material Deposition Method
Authors: Tihhonova Marina , Meos Andres , Airaksinen Sari , Aruväli Jaan , Sandler Topelius Niklas , Heinämäki Jyrki , Paaver Urve
Published: November 2024

Background/Objectives: An automated extrusion-based material deposition is a contemporary and rapid method for pharmaceutical dose-dispensing and preparing (printing) individualized solid dosage forms. The aim of this study was to investigate and gain knowledge of the feasibility of automated extrusion-based material deposition technology in preparing customized prednisolone (PRD)-loaded gel tablets for veterinary applications (primarily for dogs and cats). Methods: The PRD loads of the extrusion-based deposited gel tablets were 0.5% and 1.0%, and the target weights of tablets were 0.250 g, 0.500 g, and 1.000 g. The effects of the material deposition processes on the physical solid state, in vitro dissolution, and the physicochemical stability of PRD gel tablets were investigated. Results: The small-sized gel tablets presented a uniform round shape with an exceptionally smooth outer surface texture. The actual average weight of the tablets (n = 10) was very close to the target weight, showing the precision of the process. We found that PRD was in a pseudopolymorphic sesquihydrate form (instead of an initial PRD crystalline form II) in the gel tablets. In all the immediate-release gel tablets studied, more than 70% of the drug load was released within 30 min. The soft texture and dimensions of gel tablets affected the dissolution behaviour in vitro, suggesting the need for further development and standardization of a dissolution test method for such gel tablets. A short-term storage stability study revealed that the content of PRD did not decrease within 3 months. Conclusions: Automated extrusion-based material deposition is a feasible method for the rapid preparation of gel tablets intended for veterinary applications. In addition, the present technology and gel tablets could be used in pediatric and personalized medicine where precise dosing is crucial.
Identification of Fungus GZ in Buckwheat Rhizosphere and Its Promoting Effect in Buckwheat Seed Germination
Authors: Zhang Xiaona , Yang Guimin , Gu Lingdi , Liu Can , Chen Qingfu
Published: November 2024

To obtain fungal strains that enhance plant growth in the rhizosphere soil of buckwheat, we utilized morphological and molecular biological methods to identify 10 fungal strains from the rhizosphere soil and subsequently evaluated their effects on seed germination. The results demonstrated that all 10 fungal strains were classified as Isaria cateniannulata. The spores of these strains significantly enhanced the germination of buckwheat seeds, with germination rates improving by 3.46% to 700.75% compared to the control group. This study fills the gap in understanding I. cateniannulata as soil rhizosphere fungi, providing a foundation and materials for the seed coating technology of buckwheat seeds.
Novel Quaternary Ammonium Derivatives Based on Apple Pectin
Authors: Stanciu Magdalena-Cristina , Ionita Daniela , Tȋmpu Daniel , Popescu Irina , Suflet Dana Mihaela , Doroftei Florica , Tuchilus Cristina G.
Published: November 2024

New quaternary ammonium derivatives (quats) based on apple pectin (PA) were synthesized by the chemical modification of native polysaccharides with various quaternization mixtures containing epichlorohydrin (ECH) and a tertiary amine. Pectin derivatives (QPAs) were studied by elemental analysis, conductometric titration, Fourier-transform infrared spectroscopy (FTIR), and 13C nuclear magnetic resonance (13C NMR). Viscosity measurements enabled the evaluation of the viscosity average molar mass (Mv) for the unmodified polysaccharide, as well as its intrinsic viscosity ([η]) value. Dynamic light scattering (DLS) analysis revealed that the PA and its quats formed aggregates in an aqueous solution with either a unimodal (PA) or bimodal (QPAs) distribution. Scanning transmission electron microscopy analysis (STEM) of the PA and its derivatives demonstrated the presence of individual polymeric chains and aggregates in aqueous solution, with the smallest sizes being specific to amphiphilic polymers. Thermal stability, as well as wide-angle X-ray diffraction (WAXD) studies, generally indicated a lower thermal stability and crystallinity of the QPAs compared with those of the PA. Antipathogenic activity demonstrated that the PA and its derivatives exhibited effectiveness against S. aureus ATCC 25923 bacterium and C. albicans ATCC 10231 pathogenic yeast.
3D-Printed Poly(ester urethane)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/Bioglass Scaffolds for Tissue Engineering Applications
Authors: Lores Nayla J. , Aráoz Beatriz , Hung Xavier , Talou Mariano H. , Boccaccini Aldo R. , Abraham Gustavo A. , Hermida Élida B. , Caracciolo Pablo C.
Published: November 2024

Biodegradable polymers and bioceramics give rise to composite structures that serve as scaffolds to promote tissue regeneration. The current research explores the preparation of biodegradable filaments for additive manufacturing. Bioresorbable segmented poly(ester urethanes) (SPEUs) are easily printable elastomers but lack bioactivity and present low elastic modulus, making them unsuitable for applications such as bone tissue engineering. Strategies such as blending and composite filament production still constitute an important challenge in addressing SPEU limitations. In this work, SPEU-poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) blends and SPEU-PHBV-Bioglass 45S5® (BG) composite materials were processed into filaments and 3D structures. A comprehensive characterization of their morphology and thermal and mechanical properties is presented. The production of 3D structures based on SPEU-PHBV with excellent dimensional precision was achieved. Although SPEU-PHBV-BG printed structures showed some defects associated with the printing process, the physicochemical, thermal, and mechanical properties of these materials hold promise. The blend composition, BG content and particle size, processing parameters, and blending techniques were carefully managed to ensure that the mechanical behavior of the material remained under control. The incorporation of PHBV in SPEU-PHBV at 70:30 w/w and BG (5 wt%) acted as reinforcement, enhancing both the elastic modulus of the filaments and the compressive mechanical behavior of the 3D matrices. The compressive stress of the printed scaffold was found to be 1.48 ± 0.13 MPa, which is optimal for tissues such as human proximal tibial trabecular bone. Therefore, these materials show potential for use in the design and manufacture of customized structures for bone tissue engineering.
Performance and Reliability of Thermoelectric Conversion Using a Crooked Thermosyphon to Enhance Heat Transfer from Coal Fires
Authors: Bao Qingfeng , Guo Xiuting , Li Bo , Chen Wuyi , Wang Zhenping , Xiao Yang
Published: November 2024

A large amount of energy can accumulate and be stored during underground coal fires. As thermal energy cannot be easily removed using the traditional technologies of fire prevention and extinguishment, there is a potential benefit to collecting and utilizing thermal energy from coal fires and converting it to electrical energy. Thus, this work proposes a thermoelectric generator as a solution to convert thermal energy from coal fires to electrical energy. To improve the thermal energy conversion efficiency, an experimental test system was established using a thermosyphon, an electric heating module, a cooling circulation module, a thermoelectric module, and a data acquisition module. Under the condition of ensuring the same input heat and cooling boundary conditions, the influence of three factors, namely the cooling method, the connection method, and the coverage rate of thermoelectric devices, on the performance of the coal fire waste heat conversion system was studied. The results show that, compared with air cooling, water cooling provides a greater temperature difference for the thermoelectric module, and the maximum temperature difference can reach 65.90 °C. Series connection between thermoelectric devices will generate a higher open-circuit voltage and output voltage. The maximum horizontal open-circuit voltage value can reach 3.34 V, and the maximum output voltage is 2.61 V. Compared with the coverage rates of thermoelectric devices of 15.0% and 30.0%, the output power under the coverage rate of 22.5% is the largest at 0.35 W, and its thermoelectric conversion efficiency is also the largest at 0.35%. The optimal combination of thermoelectric modules obtained from the research results can provide ideas for the application of in situ coal fire prevention and control.
Hyperspectral Estimation of Leaf Nitrogen Content in White Radish Based on Feature Selection and Integrated Learning
Authors: Li Yafeng , Xu Xingang , Wu Wenbiao , Zhu Yaohui , Yang Guijun , Gao Lutao , Meng Yang , Jiang Xiangtai , Xue Hanyu
Published: November 2024

Nitrogen is the main nutrient element in the growth process of white radish, and accurate monitoring of radish leaf nitrogen content (LNC) is an important guide for precise fertilization decisions for radish in the field. Using white radish LNC monitoring as an object, research on radish nitrogen hyperspectral estimation methods was carried out based on leaf hyperspectral and field sample nitrogen data at multiple growth stages using feature selection and integrated learning algorithm models. First, the Vegetation Index (VI) was constructed from hyperspectral data. We extracted sensitive features of hyperspectral data and VI response to radish LNC based on Pearson’s feature-selection approach. Second, a stacking-integrated learning approach is proposed using machine learning algorithms such as Support Vector Machine (SVM), Random Forest (RF), and Ridge and K-Nearest Neighbor (KNN) as the base model in the first layer of the architecture, and the Lasso algorithm as the meta-model in the second layer of the architecture, to realize the hyperspectral estimation of radish LNC. The analysis results show the following: (1) The sensitive bands of the radish LNC are mainly centered around 600–700 nm and 1950 nm, and the constructed sensitive VIs are also concentrated in this band range. (2) The Stacking model with spectral features as inputs achieved good prediction accuracy at the radish spectral leaf, with R2 = 0.7, MAE = 0.16, MSE = 0.05 estimated over the whole growth stage of radish. (3) The Lasso algorithm with variable filtering function was chosen as the meta-model, which has a redundant model-selection effect on the base model and helps to improve the quality of the integrated learning framework. This study demonstrates the potential of the stacking-integrated learning method based on hyperspectral data for spectral estimation of nitrogen content in radish at multiple growth stages.