bluuebunny/crossref_metadata_2025 huggingface dataset:
Energizing Cellulose: Revealing the Latent Potential of Cellulose Nanofiber for Sustainable Supercapacitors
Authors: Ayinla Ridwan
Published: January 2025

2024 ECS H. H. Uhlig Research FellowShip – Summary Report
Electrolysis with Bipolar Membrane Electrode Assemblies in the Concurrent Presence of Carbon Dioxide and Nitrate
Authors: Huang Po-Wei
Published: January 2025

2024 ECS F. M. Becket Research Fellowship – Summary Report
Looking at Patent Law: Patenting an Invention for Decarbonized Cement Blends; A Case Study…Electrochemical Cement Production
Authors: Taylor E. Jennings , Inman Maria
Published: January 2025

IIn this installment of the “Looking at Patent Law” series, we present a case study of the prosecution events of U.S. Patent No. 12,065,379; “Decarbonized Cement Blends.” The subject invention aligns with an important focus of The Electrochemical Society (ECS) on sustainability and the technical interests of several divisions including Energy Technology (ETD), Industrial Electrochemistry and Electrochemical Engineering (IE&EE), and Physical and Analytical Electrochemistry (PAE). Additionally, the case introduces ECS members to an emerging technology of interest to industry. The case study begins with a brief synopsis of the background of the invention followed by 1) summary of several drawings and the specification of the invention, 2) inventor assignment and power of attorney designations, 3) march-in rights for government sponsored research, 4) submission of the Invention Disclosure Statement (IDS) and associated Duty of Candor, 5) summary of the non-final office action rejecting the patent application for obviousness, and 6) applicant response and allowance of the patent application. The case study illustrates the use of adding limitations from a dependent claim to the independent claim to overcome an obviousness rejection. In addition, the case illustrates an applicant-initiated request for prioritized examination for patent applications addressing environmental issues. With this case study, we hope to de-mystify the patent prosecution process and better prepare electrochemical and solid-state scientists, engineers and technologists to interact with their patent counsel regarding their inventions.
Chips in the 21st Century: Is the Revolution Over?
Authors: Kovacevic Eva
Published: January 2025

The great era of electronic revolution in the 20th century brought incredible changes in all aspects of our lives. From science and industry to everyday life, health, education, and entertainment, all aspects have been tremendously impacted forever. Chiplets or chips, or microchips, are the very heart of the new, digital era, and we cannot even imagine life without them. Electronics taken out of our lives would lead to a real nightmare scenario, impacting industries, medical procedures (e.g., surgeries), power plant operations, and our lives would be expulsed back in time.
The CHIPS and Science Act: A Perspective for Solid State Science and Technology
Authors: Misra Durga
Published: January 2025

With the CHIPS (Creating Helpful Incentives to Produce Semiconductors) and Science Act enacted by the United States in August 2022, a flurry of activities have emerged that aim at strengthening the resilience and competitive advantage of the American supply chain by increasing the domestic manufacturing of semiconductor electronic chips—the electronic devices used in automobiles, consumer electronics such as laptops and smart phones, data centers, and the defense sector. This resilience is integral to America’s economic and national security. In addition, the Act is expected to enhance research and development in government, industry, and academic institutions and to develop a workforce to meet the increased demand.
Semiconductor Dry Etching Innovation in the Era of 3D Monolithic Integration
Authors: Lill Thorsten , Fischer Andreas
Published: January 2025

Two-dimensional scaling of integrated circuits was the backbone of Moore’s law for 50 years. Today, however, as feature sizes reach physical limits, new approaches are needed. Monolithic scaling in the third dimension is one of the solutions. NAND flash memory started to grow in the vertical direction in 2014 with just 24 layers of memory cells with 128 Gbit of storage. The fact that the industry is targeting 1000 layers only 10 years later is testament to the stunning success of vertical integration. One thousand layers will enable petabyte solid state drives by 2030. Cryogenic reactive ion etch (RIE) of high aspect ratio holes in silicon-based dielectrics is one of its enabling technologies. Logic and dynamic random access memory (DRAM) are also conquering the third dimension. The structures of these devices are very complicated and demand novel selective etching processes which can remove material in the lateral direction.
Adsorption of Copper and Chromium Mixed Solution on N-Doped Biomass-Activated Carbon
Authors: Yuan Xiaodi , Gao Liang , Chen Shuang , Zheng Wanlan , Kang Boyuan , Qiao Jijuan
Published: January 2025

N-doped biomass activated carbon was prepared by direct ultrasonic method and redox method. Scanning electron microscopy, energy-dispersive spectroscopy, Brunnauer-Emmett-Teller, X-ray photoelectron spectroscopy, Zeta potential test, and other characterization methods were used to observe the pore structure, surface functional groups, elemental composition and distribution, and surface charged properties of the two samples. The adsorption experiment of Cu(II)/Cr(VI) mixed solution of two samples was carried out, and the influence of pH value, temperature, and concentration on material properties was explored. The results show that the equilibrium time, adsorption rate, and adsorption capacity of Cu(II)/Cr(VI) mixed solution are shorter, faster, and three times larger compared with the adsorption of single Cu(II) solution. The co-adsorption of Cu(ii) and Cr(vi) was observed. The adsorption capacity of Cr(vi) is not affected by the properties of the materials themselves, but increases with the increase of the initial concentration of Cu(II). The adsorption performance of activated carbon modified by redox method is better than that modified by direct ultrasonic method when adsorbing mixed solution of copper and chromium ions.
Table S6 from H4K20me3-Mediated Repression of Inflammatory Genes Is a Characteristic and Targetable Vulnerability of Persister Cancer Cells
Authors: Ramponi Valentina , Richart Laia , Kovatcheva Marta , Stephan-Otto Attolini Camille , Capellades Jordi , Lord Alice E. , Yanes Oscar , Ficz Gabriella , Serrano Manuel
Published: January 2025

Differentially expressed genes commonly upregulated in INK128 treated A549 and SK-Mel-147 cells with a p value < 0.05.
Table S4 from H4K20me3-Mediated Repression of Inflammatory Genes Is a Characteristic and Targetable Vulnerability of Persister Cancer Cells
Authors: Ramponi Valentina , Richart Laia , Kovatcheva Marta , Stephan-Otto Attolini Camille , Capellades Jordi , Lord Alice E. , Yanes Oscar , Ficz Gabriella , Serrano Manuel
Published: January 2025

Oligos sequences for Tn5 production
Figure S4 from H4K20me3-Mediated Repression of Inflammatory Genes Is a Characteristic and Targetable Vulnerability of Persister Cancer Cells
Authors: Ramponi Valentina , Richart Laia , Kovatcheva Marta , Stephan-Otto Attolini Camille , Capellades Jordi , Lord Alice E. , Yanes Oscar , Ficz Gabriella , Serrano Manuel
Published: January 2025

Supplementary figure 4
Figure S5 from TCTN1 Induces Fatty Acid Oxidation to Promote Melanoma Metastasis
Authors: Li Yinlam , Ming Ren , Zhang Tianyi , Gao Zixu , Wang Lu , Yang Yang , Shen Kangjie , Wei Chenlu , Zhu Yu , Li Jianrui , Zheng Shaoluan , Luo Zucheng , Ding Yiteng , Xuan Jiangying , Hu Qianrong , Yang Yanwen , Gu Jianying , Wei Chuanyuan
Published: January 2025

Figure S5
Supplementary Tables from SpatialDeX Is a Reference-Free Method for Cell-Type Deconvolution of Spatial Transcriptomics Data in Solid Tumors
Authors: Liu Xinyi , Tang Gongyu , Chen Yuhao , Li Yuanxiang , Li Hua , Wang Xiaowei
Published: January 2025

Supplementary Tables S1-S2
Supplementary Figure S6 from SpatialDeX Is a Reference-Free Method for Cell-Type Deconvolution of Spatial Transcriptomics Data in Solid Tumors
Authors: Liu Xinyi , Tang Gongyu , Chen Yuhao , Li Yuanxiang , Li Hua , Wang Xiaowei
Published: January 2025

Supplementary Figure S6
Figure 5 from Replication Stress Is an Actionable Genetic Vulnerability in Desmoplastic Small Round Cell Tumors
Authors: Kawai-Kawachi Asuka , Lenormand Madison M. , Astier Clémence , Herbel Noé , Cutrona Meritxell B. , Ngo Carine , Garrido Marlène , Eychenne Thomas , Dorvault Nicolas , Bordelet Laetitia , Song Feifei , Bouyakoub Ryme , Loktev Anastasia , Romo-Morales Antonio , Henon Clémence , Colmet-Daage Léo , Vibert Julien , Drac Marjorie , Brough Rachel , Schwob Etienne , Martella Oliviano , Pinna Guillaume , Shipley Janet M. , Mittnacht Sibylle , Zimmermann Astrid , Gulati Aditi , Mir Olivier , Le Cesne Axel , Faron Matthieu , Honoré Charles , Lord Christopher J. , Chabanon Roman M. , Postel-Vinay Sophie
Published: January 2025

EWS–WT1 drives enhanced DNA replication stress and R-loops, which contribute to DSRCT cells’ sensitivity to PARPi and ATRi. A, Assessment of replication fork speed (kb/minute) in JN1 cells subjected to siRNA-mediated silencing of EWS–WT1 or CCND1. A minimum of 50 forks was analyzed per condition. Mean ± SD; each dot represents a single replication fork; n = 2, one-way ANOVA and post hoc Dunnett test. B, Assessment of replication fork speed (kb/minute) in JN1 cells exposed to DMSO control, or a combination of PARPi talazoparib (Tala) and ATRi M4344 for 6 hours, in the presence or absence of siRNA-mediated silencing of EWS–WT1. A minimum of 50 forks was analyzed per condition. Mean ± SD; each dot represents a single replication fork; n = 2; two-way ANOVA and post hoc Šídák test. C and D, DNA:RNA hybrid dot blot of genomic DNA extracted from JN1 (C) or R (D) cells exposed to PARPi talazoparib, ATRi M4344, or a combination of both in the presence or absence of siRNA-mediated silencing of EWS–WT1 as in B. S9.6, RNA:DNA hybrids; ssDNA, loading control. E, Assessment of replication fork speed (kb/minute) in RNase H1–overexpressing JN1 cells subjected to siRNA-mediated silencing of EWS–WT1. Synchronized cells were collected 14 hours after transfection. A minimum of 50 forks was analyzed per condition. Mean ± SD; each dot represents a single replication fork; n = 2; unpaired t test. E, Dose–response survival curves of JN1 cells exposed to PARPi talazoparib (F) or olaparib (G), and ATRi M4344 (H) or AZD6738 (I) for 7 days in the presence or absence of siRNA-mediated silencing of EWS–WT1 and/or RNase H1 overexpression. Mean ± SD; n = 3; two-way ANOVA. *, P < 0.05; ****, P < 0.0001; ns, not significant.
Figure 2 from Replication Stress Is an Actionable Genetic Vulnerability in Desmoplastic Small Round Cell Tumors
Authors: Kawai-Kawachi Asuka , Lenormand Madison M. , Astier Clémence , Herbel Noé , Cutrona Meritxell B. , Ngo Carine , Garrido Marlène , Eychenne Thomas , Dorvault Nicolas , Bordelet Laetitia , Song Feifei , Bouyakoub Ryme , Loktev Anastasia , Romo-Morales Antonio , Henon Clémence , Colmet-Daage Léo , Vibert Julien , Drac Marjorie , Brough Rachel , Schwob Etienne , Martella Oliviano , Pinna Guillaume , Shipley Janet M. , Mittnacht Sibylle , Zimmermann Astrid , Gulati Aditi , Mir Olivier , Le Cesne Axel , Faron Matthieu , Honoré Charles , Lord Christopher J. , Chabanon Roman M. , Postel-Vinay Sophie
Published: January 2025

PARPi and ATRi have synergistic cytotoxic effects in models of DSRCT with high PARP1 expression. A and B, PARP1 expression (A) and PARylation levels (B) as assessed by IHC in a cohort of 16 DSRCT samples, compared with those of the JN1 and R cell lines (PARP1 and PAR expression levels are shown as H-scores). Representative cases (PARP1-high vs. PARP1-low tumors; PAR-high vs. PAR-low tumors) are shown to the right, compared with JN1 and R cells. C and D, Surface plots of Bliss independence scores calculated for the talazoparib–M4344 combination in JN1 (C) and R (D) cell lines at 7 days. E, The GR_13-PDX-O model was established from the primary peritoneal tumor of a patient with DSRCT, with confirmation of EWSR1::WT1 fusion by FISH and WT1-Cter IHC (Supplementary Fig. S8). F, Surface plot of Bliss independence scores calculated for the talazoparib–M4344 combination in the GR_13 PDX-O at 7 days. Mean ± SD; n = 3. Surface plots: the x-axis and y-axis values indicate drug concentrations, and the z-axis values indicate the associated synergy score; score < −10, antagonistic interaction; score = 0, absence of interaction; score > 10, synergistic interaction. G, Schematic illustration of an in vivo therapeutic experiment performed to evaluate the antitumor effect of PARPi talazoparib and ATRi M1774 in NSG mice engrafted with JN1 xenografts. H, Therapeutic responses to drug treatment in mice harboring JN1 xenografts. Mean tumor volume ± SD; two-way ANOVA and post hoc Dunnett test. I, Tumor volume at the time of mice sacrifice. Mean ± SD; one-way ANOVA and post hoc Šídák test. *, P < 0.01; ns, not significant. Tala, talazoparib.
Supplementary Figure S6 from LSD1 and CoREST2 Potentiate STAT3 Activity to Promote Enteroendocrine Cell Differentiation in Mucinous Colorectal Cancer
Authors: Ladaika Christopher A. , Ghobashi Ahmed H. , Boulton William C. , Miller Samuel A. , O’Hagan Heather M.
Published: January 2025

Enteric neuron-like cells are a non-secretory cell with possible clinical relevance in colorectal cancer.
Supplementary Figure S4 from LSD1 and CoREST2 Potentiate STAT3 Activity to Promote Enteroendocrine Cell Differentiation in Mucinous Colorectal Cancer
Authors: Ladaika Christopher A. , Ghobashi Ahmed H. , Boulton William C. , Miller Samuel A. , O’Hagan Heather M.
Published: January 2025

CoREST2 promotes EEC differentiation.
Figure S13 from SOS1 Inhibition Enhances the Efficacy of KRASG12C Inhibitors and Delays Resistance in Lung Adenocarcinoma
Authors: Daley Brianna R. , Sealover Nancy E. , Finniff Bridget A. , Hughes Jacob M. , Sheffels Erin , Gerlach Daniel , Hofmann Marco H. , Kostyrko Kaja , LaMorte Joseph P. , Linke Amanda J. , Beckley Zaria , Frank Andrew M. , Lewis Robert E. , Wilkerson Matthew D. , Dalgard Clifton L. , Kortum Robert L.
Published: January 2025

G12Ci DTPs show reduced sensitivity to G12Ci that is restored by SOS1i or ALDHi treatment.
Figure S12 from SOS1 Inhibition Enhances the Efficacy of KRASG12C Inhibitors and Delays Resistance in Lung Adenocarcinoma
Authors: Daley Brianna R. , Sealover Nancy E. , Finniff Bridget A. , Hughes Jacob M. , Sheffels Erin , Gerlach Daniel , Hofmann Marco H. , Kostyrko Kaja , LaMorte Joseph P. , Linke Amanda J. , Beckley Zaria , Frank Andrew M. , Lewis Robert E. , Wilkerson Matthew D. , Dalgard Clifton L. , Kortum Robert L.
Published: January 2025

G12Ci DTPs show reduced G12Ci engagement after washout.
Figure S1 from SOS1 Inhibition Enhances the Efficacy of KRASG12C Inhibitors and Delays Resistance in Lung Adenocarcinoma
Authors: Daley Brianna R. , Sealover Nancy E. , Finniff Bridget A. , Hughes Jacob M. , Sheffels Erin , Gerlach Daniel , Hofmann Marco H. , Kostyrko Kaja , LaMorte Joseph P. , Linke Amanda J. , Beckley Zaria , Frank Andrew M. , Lewis Robert E. , Wilkerson Matthew D. , Dalgard Clifton L. , Kortum Robert L.
Published: January 2025

SOS1i synergizes with G12Ci under both 2D and 3D culture conditions.
Figure 7 from Distant Metastases of Breast Cancer Resemble Primary Tumors in Cancer Cell Composition but Differ in Immune Cell Phenotypes
Authors: Kuett Laura , Bollhagen Alina , Tietscher Sandra , Sobottka Bettina , Eling Nils , Varga Zsuzsanna , Moch Holger , de Souza Natalie , Bodenmiller Bernd
Published: January 2025

Spatial T and myeloid CNs are shared between primary tissue and metastatic sites. A, Relative enrichment of T-cell (top) and myeloid (bottom) subtypes in tumor vs. stromal patches across the whole cohort (n = 87). B, Heatmaps illustrating cell-type frequencies per tumor (left), T-cell (middle), or myeloid cell (right) neighborhood. Columns are z-scored for visualization purposes. The corresponding bubble diagrams show significant results (FDR < 0.05) of differential abundance tests comparing the CNs in metastases to those in PT. Diameter reflects the log2-fold change in abundance (n = 76). moDC, monocyte-derived DCs; pDC, plasmacytoid dendritic cells.
Figure 5 from Distant Metastases of Breast Cancer Resemble Primary Tumors in Cancer Cell Composition but Differ in Immune Cell Phenotypes
Authors: Kuett Laura , Bollhagen Alina , Tietscher Sandra , Sobottka Bettina , Eling Nils , Varga Zsuzsanna , Moch Holger , de Souza Natalie , Bodenmiller Bernd
Published: January 2025

Comparison of the immune environment in PT and metastatic sites. A, Compositional similarity of the PT and metastatic samples displayed using PCA on the per-patient immune cell abundances (n = 76 patients; after applying the centered log-ratio transformation). Each point represents a single patient sample of either PT or metastatic (M) site. B, Immune cell type enrichment in PT or metastasis determined via differential abundance testing using paired design. Bar plot shows the log2-fold abundance changes (n = 76 patients). C, Compositional similarity between each individual patient-matched PT and metastatic sample based on proportional abundance of simple immune composition. The similarity was determined with Aitchison distance. Distribution of median Aitchison distance calculated over all the samples after every random permutation (n = 1,000 iterations) of sample labels. P value was calculated as the probability of observing a permutation median lower than the true patient cohort median. The dashed lines show the median Aitchison distance for the patient cohort and the mean of median distances for the randomly permuted iterations. D–I, As in A–C, for T-cell subtypes (D–F) and myeloid cell subsets (G–I). moDC, monocyte-derived DCs; pDC, plasmacytoid dendritic cells.
Figure 3 from Distant Metastases of Breast Cancer Resemble Primary Tumors in Cancer Cell Composition but Differ in Immune Cell Phenotypes
Authors: Kuett Laura , Bollhagen Alina , Tietscher Sandra , Sobottka Bettina , Eling Nils , Varga Zsuzsanna , Moch Holger , de Souza Natalie , Bodenmiller Bernd
Published: January 2025

Phenotypic composition analysis of tumor cells between PT and distant metastasis. A, Flow chart depicting the most common phenotypic cluster in PT vs. metastatic sample. Stacked bar charts show the frequency of each cluster across the patient cohort and lines connect matched patient samples. B, Compositional similarity of the PT and metastatic samples displayed using PCA on the per-patient phenotypic tumor cell cluster abundances as a proportion out of all the epithelial cells (abundances were transformed with the centered log-ratio transformation). Each point represents a single patient sample of either PT or metastatic site (n = 76 patients). C, Pairwise phenotypic similarity (Aitchison distance) for the clinical cohort and randomly permuted pairwise samples, with dashed lines showing the median Aitchison distance for the patient cohort and the mean of median distances for the randomly permuted iterations (left). The right plot shows pairwise phenotypic similarity for matched samples grouped by the site of metastasis. The P value indicates no significant difference across all groups (Kruskal–Wallis test).
Supplementary Table 2 from PARP7 Inhibitors and AHR Agonists Act Synergistically across a Wide Range of Cancer Models
Authors: Chen Huadong , Gou Xuxu , Mao Ying , O’Leary Patrick C. , Diolaiti Morgan E. , Ashworth Alan
Published: January 2025

Supplementary Table 2. Primers used for construction of lentiCRISPRv2 sgRNA plasmids.
Figure S1 from A Novel Lipid Nanoparticle NBF-006 Encapsulating Glutathione S-Transferase P siRNA for the Treatment of KRAS-Driven Non–small Cell Lung Cancer
Authors: Cina Cima , Majeti Bharat , O’Brien Zhihong , Wang Li , Clamme Jean Pierre , Adami Roger , Tsang Kwok Yin , Harborth Jens , Ying Wenbin , Zabludoff Sonya
Published: January 2025

non-GLP mouse tox
Supplementary Figure 3 from PARP7 Inhibitors and AHR Agonists Act Synergistically across a Wide Range of Cancer Models
Authors: Chen Huadong , Gou Xuxu , Mao Ying , O’Leary Patrick C. , Diolaiti Morgan E. , Ashworth Alan
Published: January 2025

Supplementary Fig. 3 PARP7 knockout or inhibition has a similar synergistic lethal effect with AHR agonists
Figure S2 from A Small-Molecule BCL6 Inhibitor as an Anti-Proliferative Agent for Diffuse Large B-Cell Lymphoma
Authors: Xing Yajing , Guo Weikai , Wu Min , Xie Jiuqing , Huang Dongxia , Hu Pan , Zhou Miaoran , Zhang Lin , Zhong Yadong , Liu Mingyao , Chen Yihua , Yi Zhengfang
Published: January 2025

Figure S2. WK692 specifically inhibits BCL6 but not other BTB-ZF Proteins. Reporter assays were performed to test the activity of WK692 with BCL6, Kaiso and PLZF. (***, P < 0.001 versus control).
Figure S1 from A Small-Molecule BCL6 Inhibitor as an Anti-Proliferative Agent for Diffuse Large B-Cell Lymphoma
Authors: Xing Yajing , Guo Weikai , Wu Min , Xie Jiuqing , Huang Dongxia , Hu Pan , Zhou Miaoran , Zhang Lin , Zhong Yadong , Liu Mingyao , Chen Yihua , Yi Zhengfang
Published: January 2025

Figure S1. Chemical structures of FX1, BI3802 and WK692.
Supplemental Figure 5 from Tumor-Specific Antigen Delivery for T-cell Therapy via a pH-Sensitive Peptide Conjugate
Authors: Yurkevicz Annali M. , Liu Yanfeng , Katz Samuel G. , Glazer Peter M.
Published: January 2025

Luminex cytokine profiling of serum obtained from OT1 mice treated with pHLIP-SIINFEKL or controls. Blood was harvested after three treatments and analyzed for the indicated cytokine levels (n=3).
Figure S8 from Zelenirstat Inhibits N-Myristoyltransferases to Disrupt Src Family Kinase Signaling and Oxidative Phosphorylation, Killing Acute Myeloid Leukemia Cells
Authors: Gamma Jay M. , Liu Qiang , Beauchamp Erwan , Iyer Aishwarya , Yap Megan C. , Zak Zoulika , Ekstrom Cassidy , Pain Rony , Kostiuk Morris A. , Mackey John R. , Brandwein Joseph , Wang Jean C.Y. , Berthiaume Luc G.
Published: January 2025

Figure S8: Zelenirstat disrupts signalling in AML. U937 and KG-1 cells were incubated with zelenirstat for 48 hours, stimulated with 100 ng/mL FL & SCF, then lysed. Lysates were probed for HCK, phosphorylated SFKs, and phosphorylated Stat5 by western blotting. Band intensity was determined using Image Studio Lite, and each sample normalized to actin. Differences between treatment groups determined using one-way ANOVA.
Figure S5 from Zelenirstat Inhibits N-Myristoyltransferases to Disrupt Src Family Kinase Signaling and Oxidative Phosphorylation, Killing Acute Myeloid Leukemia Cells
Authors: Gamma Jay M. , Liu Qiang , Beauchamp Erwan , Iyer Aishwarya , Yap Megan C. , Zak Zoulika , Ekstrom Cassidy , Pain Rony , Kostiuk Morris A. , Mackey John R. , Brandwein Joseph , Wang Jean C.Y. , Berthiaume Luc G.
Published: January 2025

Supplementary Figure S5: MISS-54 Scores Categorized by AML Risk Category TCGA-LAML patients were separated into groups by ELN2022 risk classification (A) or TCGA-LAML molecular risk category (B) and MISS-54 scores of groups compared by one-way ANOVA. No significant differences were found between groups.
Figure S4 from Zelenirstat Inhibits N-Myristoyltransferases to Disrupt Src Family Kinase Signaling and Oxidative Phosphorylation, Killing Acute Myeloid Leukemia Cells
Authors: Gamma Jay M. , Liu Qiang , Beauchamp Erwan , Iyer Aishwarya , Yap Megan C. , Zak Zoulika , Ekstrom Cassidy , Pain Rony , Kostiuk Morris A. , Mackey John R. , Brandwein Joseph , Wang Jean C.Y. , Berthiaume Luc G.
Published: January 2025

Supplementary Figure S4: Expression of NMT1 and NMT2 as it associates with AML risk factors. Patients were separated based on mutational status of commonly used prognostic genes and compared based on expression of NMT1 (A) and NMT2 (B). No significant differences were found in displayed genes. Patients were separated into risk categories based on ELN2022 guidelines or TCGA-LAML molecular risk and expression of NMT1 and NMT2 evaluated (C). No significant differences were found.
Figure S2 from Zelenirstat Inhibits N-Myristoyltransferases to Disrupt Src Family Kinase Signaling and Oxidative Phosphorylation, Killing Acute Myeloid Leukemia Cells
Authors: Gamma Jay M. , Liu Qiang , Beauchamp Erwan , Iyer Aishwarya , Yap Megan C. , Zak Zoulika , Ekstrom Cassidy , Pain Rony , Kostiuk Morris A. , Mackey John R. , Brandwein Joseph , Wang Jean C.Y. , Berthiaume Luc G.
Published: January 2025

Supplementary Figure S2: Zelenirstat effectively inhibits myristoylation in AML cells. Cells pre-incubated with zelenirstat at indicated concentrations for 1 hour were metabolically labeled with alkynyl-myristate analog. Incorporation of alkynyl-myristate analog into proteins was measured by Western Blotting after click reaction with azido-biotin using streptavidin-HRP/ECL.
Supplementary Methods from Riluzole Enhancing Anti-PD-1 Efficacy by Activating cGAS/STING Signaling in Colorectal Cancer
Authors: Liang Beiyuan , Khan Misbah , Storts Hayden , Zhang Evan H. , Zheng Xinru , Xing Xuanxuan , Claybon Hazel , Wilson Jenna , Li Chunjie , Jin Ning , Fishel Richard , Miles Wayne O. , Wang Jing J.
Published: January 2025

Supplemental methods
Supplementary Figure 2 from Riluzole Enhancing Anti-PD-1 Efficacy by Activating cGAS/STING Signaling in Colorectal Cancer
Authors: Liang Beiyuan , Khan Misbah , Storts Hayden , Zhang Evan H. , Zheng Xinru , Xing Xuanxuan , Claybon Hazel , Wilson Jenna , Li Chunjie , Jin Ning , Fishel Richard , Miles Wayne O. , Wang Jing J.
Published: January 2025

Supplemental figure 2 and legend
Supplementary Table S2 from PET Imaging Using 89Zr-Labeled StarPEG Nanocarriers Reveals Heterogeneous Enhanced Permeability and Retention in Prostate Cancer
Authors: Meher Niranjan , Bidkar Anil P. , Wadhwa Anju , Bobba Kondapa Naidu , Dhrona Suchi , Dasari Chandrashekhar , Mu Changhua , Fong Cyril O.Y. , Cámara Juan A. , Ali Umama , Basak Megha , Bulkley David , Steri Veronica , Fontaine Shaun D. , Zhu Jun , Oskowitz Adam , Aggarwal Rahul R. , Sriram Renuka , Chou Jonathan , Wilson David M. , Seo Youngho , Santi Daniel V. , Ashley Gary W. , VanBrocklin Henry F. , Flavell Robert R.
Published: January 2025

Supplementary Table S2. Organ biodistribution data in %ID/g tissue for [89Zr]PEG-DFB1-TLZ3 at 72 h in mice model bearing different subcutaneous tumors. (n = 4)
Supplementary Table S14 from PET Imaging Using 89Zr-Labeled StarPEG Nanocarriers Reveals Heterogeneous Enhanced Permeability and Retention in Prostate Cancer
Authors: Meher Niranjan , Bidkar Anil P. , Wadhwa Anju , Bobba Kondapa Naidu , Dhrona Suchi , Dasari Chandrashekhar , Mu Changhua , Fong Cyril O.Y. , Cámara Juan A. , Ali Umama , Basak Megha , Bulkley David , Steri Veronica , Fontaine Shaun D. , Zhu Jun , Oskowitz Adam , Aggarwal Rahul R. , Sriram Renuka , Chou Jonathan , Wilson David M. , Seo Youngho , Santi Daniel V. , Ashley Gary W. , VanBrocklin Henry F. , Flavell Robert R.
Published: January 2025

Supplementary Table S14. Comparison of influx and efflux rates of [89Zr]PEG-DFB4 and [89Zr]PEG-DFB1-TLZ3 conjugates in different subcutaneous tumor models (n = 4, mean ± SD). *Prior published data.6
Supplementary Table S1 from PET Imaging Using 89Zr-Labeled StarPEG Nanocarriers Reveals Heterogeneous Enhanced Permeability and Retention in Prostate Cancer
Authors: Meher Niranjan , Bidkar Anil P. , Wadhwa Anju , Bobba Kondapa Naidu , Dhrona Suchi , Dasari Chandrashekhar , Mu Changhua , Fong Cyril O.Y. , Cámara Juan A. , Ali Umama , Basak Megha , Bulkley David , Steri Veronica , Fontaine Shaun D. , Zhu Jun , Oskowitz Adam , Aggarwal Rahul R. , Sriram Renuka , Chou Jonathan , Wilson David M. , Seo Youngho , Santi Daniel V. , Ashley Gary W. , VanBrocklin Henry F. , Flavell Robert R.
Published: January 2025

Supplementary Table S1. Region of interest analysis data reported as %ID/cc of the [89Zr]PEG-DFB1-TLZ3 on on different subcutaneous tumors on 24 h and 72 h post injection. (n = 4)
Supplementary Figure S20 from PET Imaging Using 89Zr-Labeled StarPEG Nanocarriers Reveals Heterogeneous Enhanced Permeability and Retention in Prostate Cancer
Authors: Meher Niranjan , Bidkar Anil P. , Wadhwa Anju , Bobba Kondapa Naidu , Dhrona Suchi , Dasari Chandrashekhar , Mu Changhua , Fong Cyril O.Y. , Cámara Juan A. , Ali Umama , Basak Megha , Bulkley David , Steri Veronica , Fontaine Shaun D. , Zhu Jun , Oskowitz Adam , Aggarwal Rahul R. , Sriram Renuka , Chou Jonathan , Wilson David M. , Seo Youngho , Santi Daniel V. , Ashley Gary W. , VanBrocklin Henry F. , Flavell Robert R.
Published: January 2025

Supplementary Figure S20. Organ biodistribution presented in (left) %ID/g, and (right) %ID/organ of [89Zr]PEG-DFB1-TLZ3 in nude mice inoculated with 22rv1 cells in the left kidney capsule at 96 h postinjection. (n = 4, mean ± SD)
Supplementary Figure 4 from Targeting Tumor Antigen 5T4 Using CAR T Cells for the Treatment of Acute Myeloid Leukemia
Authors: Harrop Richard , Blount Daniel G. , Khan Naeem , Soyombo Mayowa , Moyce Laura , Drayson Mark T. , Down Jenny , Lawson Michelle A. , O’Connor Deirdre , Nimmo Rachael , Lad Yatish , Souberbielle Bernard , Mitrophanous Kyriacos , Ettorre Anna
Published: January 2025

Supplementary Figure 4: 5T4 expression in normal tissues (Materials and Methods).
Supplementary Figure 1 from Targeting Tumor Antigen 5T4 Using CAR T Cells for the Treatment of Acute Myeloid Leukemia
Authors: Harrop Richard , Blount Daniel G. , Khan Naeem , Soyombo Mayowa , Moyce Laura , Drayson Mark T. , Down Jenny , Lawson Michelle A. , O’Connor Deirdre , Nimmo Rachael , Lad Yatish , Souberbielle Bernard , Mitrophanous Kyriacos , Ettorre Anna
Published: January 2025

Supplementary Figure 1A. Gating Strategy for the analysis of AML samples. Supplementary Figure 1B. 5T4 surface expression in selected AML patient samples using Multi-parameter Flow Cytometry.
Figure S3 from Tumor Integrin-Targeted Glucose Oxidase Enzyme Promotes ROS-Mediated Cell Death that Combines with Interferon Alpha Therapy for Tumor Control
Authors: Stinson Jordan A. , Sheen Allison , Lax Brianna M. , Yang Grace N. , Duhamel Lauren , Santollani Luciano , Fink Elizabeth , Palmeri Joseph R. , Wittrup Karl Dane
Published: January 2025

Cross-presentation assay T-cell gating strategy
Supplementary Table S5 from Loss of PI5P4Kα Slows the Progression of a Pten Mutant Basal Cell Model of Prostate Cancer
Authors: Triscott Joanna , Lehner Marika , Benjak Andrej , Reist Matthias , Emerling Brooke M. , Ng Charlotte K.Y. , de Brot Simone , Rubin Mark A.
Published: January 2025

Table S5. Metabolomics summary
Supplementary Table S2 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype
Authors: Ippolito Joseph E. , Hartig Jordan P. , Bejar Kaitlyn , Nakhoul Hani , Sehn Jennifer K. , Weimholt Cody , Grimsley Grace , Nunez Elena , Trikalinos Nikolaos A. , Chatterjee Deyali , Kim Eric H. , Mehta Anand S. , Angel Peggi M. , Troyer Dean A. , Leach Robin J. , Corey Eva , Wu Jennifer D. , Drake Richard R.
Published: January 2025

Table S2. Patient data from the University of Texas Health San Antonio Tissue Microarray.
Table S8 from The Distinct Roles of NEIL1 and XPA in Limiting Aflatoxin B1–Induced Mutagenesis in Mice
Authors: Luzadder Michael M. , Minko Irina G. , Vartanian Vladimir L. , Davenport Marten , Fedorov Lev M. , McCullough Amanda K. , Lloyd R. Stephen
Published: January 2025

Trinucleotide mutation spectra in aflatoxin-exposed mice
Supplementary video 1 from B-type Plexins Regulate Mitosis via RanGTPase
Authors: Mukhwana Nicholus , Garg Ritu , Azad Abul , Mitchell Alexandria R. , Williamson Magali
Published: January 2025

HeLa cells expressing GFP-tubulin and mCherry-histone transfected with control siRNA, (control for video 2). 10 min frames, total time of 90 min, x60 magnification.
Supplementary Figure 4 from B-type Plexins Regulate Mitosis via RanGTPase
Authors: Mukhwana Nicholus , Garg Ritu , Azad Abul , Mitchell Alexandria R. , Williamson Magali
Published: January 2025

B-type Plexin depletion increases unattached kinetochores
Table S3 from The Distinct Roles of NEIL1 and XPA in Limiting Aflatoxin B1–Induced Mutagenesis in Mice
Authors: Luzadder Michael M. , Minko Irina G. , Vartanian Vladimir L. , Davenport Marten , Fedorov Lev M. , McCullough Amanda K. , Lloyd R. Stephen
Published: January 2025

Spontaneous mutation subtypes
Table S1 from The Distinct Roles of NEIL1 and XPA in Limiting Aflatoxin B1–Induced Mutagenesis in Mice
Authors: Luzadder Michael M. , Minko Irina G. , Vartanian Vladimir L. , Davenport Marten , Fedorov Lev M. , McCullough Amanda K. , Lloyd R. Stephen
Published: January 2025

Locations and regions of targets
Figure S2 from Depletion of Adipose Stroma-Like Cancer-Associated Fibroblasts Potentiates Pancreatic Cancer Immunotherapy
Authors: Rupert Joseph , Daquinag Alexes , Yu Yongmei , Dai Yulin , Zhao Zhongming , Kolonin Mikhail G.
Published: January 2025

The effects of Pdgfrb+ CAF vs ASC-like CAF depletion on the TME. A, tumor section immunofluorescence for CD3 (Red) expression to measure T-cell infiltration and nuclei (blue) (left), CD3+ cell quantification (right). B, tumor section immunofluorescence for endomucin (green) to quantify endothelial cell density and nuclei (blue) (left), endomucin quantification (right). In A-B, for each tumor, a minimum of 15 fields (10X) were taken and quantified using Image J. Images were converted to 8-bit and a threshold was applied to only highlight endomucin expression and percent area was measured. An average was calculated for all images per tumor. Scale bar=100μm. *=p<0.05, ***=p<0.001.C, Volcano plots generated by IPA analysis of scRNAseq data in Fig. 3 identifying top genes induced (red) or suppressed (green) in KPC cells and CAFs from mice subjected to Pdgfrb+ CAF or ASC-like CAF depletion. D, Lists of the most upregulated (top) and downregulated (bottom) genes.
Figure 2 from Depletion of Adipose Stroma-Like Cancer-Associated Fibroblasts Potentiates Pancreatic Cancer Immunotherapy
Authors: Rupert Joseph , Daquinag Alexes , Yu Yongmei , Dai Yulin , Zhao Zhongming , Kolonin Mikhail G.
Published: January 2025

The effect of ASC-like CAF depletion on orthotopic PDAC progression. Tissues resected from male mice after 25 days of D-CAN (N = 3) or control PBS (N = 3) treatment were analyzed. A, KPC tumor size upon resection. B, Representative resected KPC tumors. C, Paraffin sections of tumors in B stained with hematoxylin/eosin, trichrome blue, or subjected to αSMA and endomucin immunofluorescence. Arrows indicate examples of well-differentiated KPC cells forming cancerous ducts. Scale bar, 50 μm. D, Quantification of KPC cells in livers based on colony counts. Images: representative wells. *, P < 0.05; **, P < 0.01; ****, P < 0.001, Student t test. H&E, hematoxylin and eosin.
Data from Depletion of Adipose Stroma-Like Cancer-Associated Fibroblasts Potentiates Pancreatic Cancer Immunotherapy
Authors: Rupert Joseph , Daquinag Alexes , Yu Yongmei , Dai Yulin , Zhao Zhongming , Kolonin Mikhail G.
Published: January 2025

Abstract
Immune checkpoint blockade therapy, transformative in some cancer types, has remained ineffective for patients with pancreatic cancer. The effects of subpopulations of cancer-associated fibroblasts (CAF) on cancer progression and therapy resistance are incompletely understood. In this study, the roles of CAFs expressing platelet-derived growth factor receptor β (Pdgfrb) and of CAFs expressing markers of adipose stromal cells (ASC) were analyzed in mice with pancreatic ductal adenocarcinoma. Ablation of Pdgfrb+ cells resulted in suppression of primary pancreatic tumor growth, reduction of extracellular matrix deposition, and increased cancer cell metastasis to the liver. A peptide D-CAN, which induces apoptosis in ASC-like CAFs, also reduced pancreatic tumor growth and extracellular matrix deposition while promoting metastases. Single-cell RNA sequencing demonstrated that depletion of either Pdgfrb+ or ASC-like CAFs decreased frequencies of tumor endothelial cells and viable cancer cells. However, whereas depletion of Pdgfrb+ CAFs led to stronger induction of cancer cell aggressiveness markers, depletion of ASC-like CAFs had an opposite effect on remaining CAFs. Depletion of ASC-like CAFs using D-CAN also led to higher infiltration of cytotoxic T-lymphocytes and B-lymphocytes. Administration of anti-PDL1 antibody (aPDL1), which inhibits the immune checkpoint, had a stronger suppressive effect on tumor growth when combined with D-CAN in both female and male mice. Liver metastases were also reduced by the D-CAN/aPDL1 combination more effectively than by aPDL1 alone in female mice. We conclude that improved approaches to target ASC-like CAFs may be effective in combination with immunotherapy.
Significance:
This study shows that populations of CAFs have distinct effects on pancreatic cancer progression and shows that depletion of CAFs expressing adipose markers potentiates tumor/metastasis suppression effects of immune checkpoint blockade.
Third Trimester Development of Central Autonomic Network Connectivity is Altered in an Extrauterine Environment
Authors: Christoffel Kelsey , De Asis Cruz Josepheen , Cook Kevin Michael , Kapse Kushal , Andescavage Nickie , Basu Sudeepta , Limperopoulos Catherine , du Plessis Adre
Published: January 2025

Introduction: The Central Autonomic Network (CAN), which involves complex interconnected brain regions that modulate the autonomic nervous system, may be key to understanding higher risk for psychosocial and behavioral challenges in preterm neonates.

Methods: We compared resting state functional connectivity of the CAN in 94 healthy term-born controls and 94 preterm infants at term-equivalent age (TEA). In preterm infants we correlated CAN connectivity with postmenstrual age (PMA). The preterm cohort underwent the Infant Toddler Social and Emotional Assessment at 18-month follow-up and these scores were correlated with CAN connectivity.

Results: CAN connectivity at the amygdala (p<.001), hippocampus (p<.001), insula (p<.001), brainstem (p=.003), and thalamus (p= .032) was significantly higher in term (n=94) than preterm (n=94) neonates. In preterm neonates, CAN connectivity positively correlated with PMA at the thalamus (r= .438, p< .001), insula (r= .304, p< .001), precuneus (r= .288, p<.001), hippocampus (r= .283, p< .001), and amygdala (r= .142, p=.034). At 18-month follow-up, (n=30, mean age 19.8 ± 3.4 months), CAN connectivity at the insula was negatively correlated with externalizing behaviors (r= -.529, p= .003).

Conclusion: In preterm neonates, the CAN evolves dynamically over the extrauterine third trimester and is measurably different compared to term-born neonates in ways that impact developmental outcomes. This is the first study to describe CAN connectivity using fMRI in large cohort of term and preterm neonates, and to report an association of CAN connectivity and behavioral outcomes.
Validation of an Application-based Cognitive Screening Test for Older Thai Adults
Authors: Yangyuensathaporn Benjapa , Chansaengpetch Supakorn , Jongsawadipatana Angkana , Muangpaisan Weerasak
Published: January 2025

Introduction
The cognitive screening usually requires a face-to-face format, which might limit its use in many circumstances. We aimed to develop a new application-based cognitive screening test (ACST) to serve as an accessible and valid tool in the community.
Methods
The ACST was developed by using paired association and digit span tests. This test was administered to 70 cognitively normal participants, 62 participants with MCI and 64 participants with dementia. The 2nd edition of the Mini-Mental State Examination (MMSE-2), and the Montreal Cognitive Assessment (MoCA) were collected by certified psychologists. The ACST was performed by a clinician. The diagnosis was made according to DSM-V criteria by an experienced geriatric neurologist blinded to the application score. Content validity, test-retest reliability, interrater reliability, and correlations between application scores and MMSE-2 and MoCA scores were analyzed.
Results
The sensitivity and specificity for distinguishing cognitively normal participants from nonnormal participants was 92.9% and 70%, respectively (cutoff point ≤ 7). The sensitivity and specificity for distinguishing between the cognitively normal group and the MCI group was 87.1% and 70%, respectively (cut point ≤ 7). The sensitivity and specificity for distinguishing cognitively normal participants from participants with dementia was 93.8% and 82.9%, respectively (cut point ≤ 6). A cutoff point ≤ 6 was considered suitable for participants aged 75 years or older or with 6 or fewer years of education.
Discussion
The ACST is an easy-to-use and valid tool for cognitive screening in older Thai adults in clinical practice. Patients with an application score ≤ 7 are considered to be at risk of cognitive impairment and to require further evaluation.
A NEW MODEL OF CHRONIC KIDNEY DISEASE, METABOLIC DERANGEMENTS, AND HEART FAILURE WITH PRESERVED EJECTION FRACTION IN AGING SWINE
Authors: Chade Alejandro R. , Tharp Darla L. , Sitz Rhys , McCarthy Elizabeth A. , Shivam Kumar , Kazeminia Sara , Eirin Alfonso
Published: January 2025

Introduction: Chronic kidney disease (CKD) and heart failure with preserved ejection fraction (HFpEF) are more prevalent in the elderly. There is a lack of large animal models that allow the study of the impact of age on CKD and HFpEF in a translational fashion. This manuscript reports the first large preclinical model of CKD-HFpEF and metabolic derangements in naturally aged swine. Methods: CKD-HFpEF was induced in naturally aged (6-9 years old) and young (3-months old) pigs, followed for 14 weeks, and compared to normal young and old controls (n=5/group). Renal and cardiac hemodynamics were quantified in vivo by multidetector-CT, echocardiography, and pressure-volume relationship studies. Renal and cardiac microvascular (MV) architecture (3D-micro-CT) and morphometric analysis (staining) were investigated ex vivo. Results: Both young and old pigs developed CKD-HFpEF, but the renal, cardiac, and metabolic phenotype was accentuated in aging animals. Aging and CKD-HFpEF influenced fasting insulin levels and insulin resistance, GFR, cortical MV density, glomerulosclerosis, perivascular fibrosis, and tubular injury. Tubule-interstitial fibrosis and peritubular capillary density were influenced by aging, CKD-HFpEF, and their interaction (2-way ANOVA). Similarly, cardiac MV density, perivascular fibrosis, and myocardial remodeling were influenced by aging and CKD-HFpEF, and E/A by their interaction. Notably, renal and cardiac MV density correlated with renal and cardiac functional and structural changes.
Conclusion: Our study establishes the first large animal model of aging CKD-HFpEF, allowing the investigation of age as a biological variable in cardio-renal and metabolic diseases. This new platform could foster new age-related research toward developing therapeutic interventions in CKD-HFpEF.
Under and Overmentalizing in Personality Disorders: A principle component analysis of nonadaptive personality and the Movie Assessment of Social Cognition
Authors: Jurist Julia , Traynor Jenna M. , Murray Grace E. , Ren Boyu , Masland Sara R. , Mermin Sam , Meehan Kevin B. , Choi-Kain Lois W.
Published: January 2025

Introduction
This secondary analysis of quality control data assessed principal components of personality dysfunction and their relationship to mentalizing in a sample of treatment-seeking women with severe personality disorders.
Methods
The Schedule for Nonadaptive and Adaptive Personality (SNAP) and the Movie for the Assessment of Social Cognition (MASC) were administered to 37 females in routine quality assessments of a specialized residential treatment program. Principal component analysis (PCA) of SNAP scores was used to determine dimensions of personality most significantly contributing to overall maladaptive personality functioning. Bootstrapped stepwise regression tested the relationship of dimensional personality indices to hypermentalizing and hypomentalizing on the MASC controlling for general psychiatric severity.
Results
Four principal components (PCs) explained 71.4% of the variance in personality dysfunction, mapping onto antisocial, obsessive-compulsive, borderline, and narcissistic personality constellations. The borderline and antisocial PCs were positively predictive of hypermentalizing. The obsessive-compulsive PC was positively predictive of hypomentalizing, while the antisocial PC was negatively predictive of hypomentalizing.
Conclusion
The study reiterates prior findings of a relationship between hypermentalizing and borderline and antisocial personality profiles. It also contributes evidence to the limited research on hypomentalizing as a clinical indicator and potential treatment target for obsessive-compulsive personality, and provides evidence of a negative relationship between antisocial personality disorder and hypomentalizing. These findings provide clinical indications for enhancing and regulating mentalizing via attention to and interpretations of internal and interpersonal events in individuals with personality disorders. Further research is needed to replicate these associations in larger, more representative clinical samples.
Prevalence of Urinary Tract Infections and Antibiotic Susceptibility Patterns of Uropathogens among Neonates in Maternity Hospital, Kuwait: A Six-year Retrospective Study
Authors: Moghnia Ola H. , Al Otaibi Hessah S. , Al Haqqan Aisha M. , Sokhn Elie S. , Pathan Seema S. , Abdulaziz Nawar E. , Mohammed Habiba Y. , Al-Sweih Noura A.
Published: January 2025

Objective: Urinary tract infections (UTIs) are common in neonates. Understanding the changes in the prevalence of common uropathogens is essential for early diagnosis and effective treatment of UTIs. This study aims to identify etiological agents and determine the local antibiotic susceptibility patterns of uropathogens causing UTIs. Subjects and Methods: A retrospective cross-sectional descriptive study from January 2017 to December 2022 was conducted on hospitalized neonates at Maternity Hospital, Kuwait. Urine samples from neonates were analyzed to identify isolates, and antimicrobial susceptibility testing was determined using the VITEK® 2 system. Results: Out of 3996 urine samples processed, 282 (7%) samples yielded significant bacteriuria, mostly from male 185 (65.6%). Gram-negative isolates were the most common 141 (50%), followed by yeasts 84 (29.8%) and Gram-positive isolates 57 (20.2%). The common uropathogens were Klebsiella pneumoniae 50 (17.7%), followed by Escherichia coli 47 (16.8%), Candida albicans 39 (13.8%), Enterococcus faecalis 34 (12%) and Staphylococcus epidermidis 17 (6%). High resistance rates were observed among Enterobacterales against ampicillin, cephalothin, cefuroxime, cefotaxime, nitrofurantoin, amoxicillin-clavulanic acid, ceftazidime and trimethoprim-sulfamethoxazole. A total of 28 (56%) K. pneumoniae and 18 (38.3%) E. coli were ESBL producers. Conclusion: Gram-negative isolates are considered the predominant causative agents of UTIs in neonates at Maternity Hospital. Reduced antibiotic susceptibility to commonly used antibiotics poses a notable challenge in the clinical management of neonates with UTIs. This study underscores the importance of proactive surveillance in monitoring causative organisms and antibiotic susceptibility in neonates.
Artificial Intelligence and Machine Learning in Preeclampsia
Authors: Layton Anita T.
Published: January 2025

Preeclampsia is a multisystem hypertensive disorder that manifests itself after 20 weeks of pregnancy, along with proteinuria. The pathophysiology of preeclampsia is incompletely understood. Artificial intelligence, especially machine learning with its capability to identify patterns in complex data, has the potential to revolutionize preeclampsia research. These data-driven techniques can improve early diagnosis, personalize risk assessment, uncover the disease’s molecular basis, optimize treatments, and enable remote monitoring. This brief review discusses the recent applications of artificial intelligence and machine learning in preeclampsia management and research, including the improvements these approaches have brought, along with their challenges and limitations.