bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainable Bacterial Control of Hatching Eggshells Using Essential Oils
Authors: Vale Igor Rafael Ribeiro , Oliveira Gabriel da Silva , de Jesus Luana Maria , de Castro Marcio Botelho , McManus Concepta , dos Santos Vinícius Machado
Published: October 2024

Background: Decontamination of poultry surfaces through appropriate hygiene and sanitation measures can partially mitigate bacterial problems, as this process does not result in the complete elimination of bacteria. Thus, the remaining bacteria can persist and contaminate eggshells. Therefore, regardless of the rigor of the sanitary standards applied on farms, it is suggested that hatching eggs be subjected to the sanitization process. Here, we investigated the effectiveness of essential oil-based antibacterial agents in sanitizing eggs. Results: The results indicated that essential oils from Cinnamomum cassia (L.) J. Presl. (CCEO), Syzygium aromaticum (L.) Merr. & L.M. Perry (SAEO) and Cymbopogon nardus (L.) Rendle (CNEO), at specific concentrations, have antibacterial effects in vitro, reducing the load of mesophilic bacteria and enterobacteria in the eggshell by at least 3 and 2 log10 CFU/mL, respectively. Conclusion: The adoption of CCEO, SAEO and CNEO to reduce the bacterial load on eggshells could be a favorable change to the conventional protocol of egg sanitization with formaldehyde, especially on farms where sanitary standards are insufficient.
Antibacterial and Antifungal Activity of Metabolites from Basidiomycetes: A Review
Authors: Lysakova Valeria , Krasnopolskaya Larissa , Yarina Maria , Ziangirova Mayya
Published: October 2024

Background/Objectives: The search for new antimicrobial molecules is important to expand the range of available drugs, as well as to overcome the drug resistance of pathogens. One of the promising sources of antibacterial and antifungal metabolites is basidial fungi, which have wide biosynthetic capabilities. Methods: The review summarized the results of studying the antimicrobial activity of extracts and metabolites from basidiomycetes published from 2018–2023. Results: In all studies, testing for antibacterial and antifungal activity was carried out in in vitro experiments. To obtain the extracts, mainly the fruiting bodies of basidiomycetes, as well as their mycelia and culture liquid were used. Antimicrobial activity was found in aqueous, methanol, and ethanol extracts. Antimicrobial metabolites of basidiomycetes were isolated mainly from the submerged culture of basidiomycetes. Metabolites active against Gram-positive and Gram-negative bacteria and mycelial and yeast-like fungi were identified. Conclusions: Basidiomycete extracts and metabolites have shown activity against collectible strains of bacteria and fungi and multi-resistant and clinical strains of pathogenic bacteria. The minimum inhibitory concentration (MIC) values of the most active metabolites ranged from 1 to 16.7 µg/mL.
Interactions Between Ferroptosis and Oxidative Stress in Ischemic Stroke
Authors: Liu Daohang , Yang Sha , Yu Shuguang
Published: October 2024

Ischemic stroke is a devastating condition that occurs due to the interruption of blood flow to the brain, resulting in a range of cellular and molecular changes. In recent years, there has been growing interest in the role of ferroptosis, a newly identified form of regulated cell death, in ischemic stroke. Ferroptosis is driven by the accumulation of lipid peroxides and is characterized by the loss of membrane integrity. Additionally, oxidative stress, which refers to an imbalance between prooxidants and antioxidants, is a hallmark of ischemic stroke and significantly contributes to the pathogenesis of the disease. In this review, we explore the interactions between ferroptosis and oxidative stress in ischemic stroke. We examine the underlying mechanisms through which oxidative stress induces ferroptosis and how ferroptosis, in turn, exacerbates oxidative stress. Furthermore, we discuss potential therapeutic strategies that target both ferroptosis and oxidative stress in the treatment of ischemic stroke. Overall, this review highlights the complex interplay between ferroptosis and oxidative stress in ischemic stroke and underscores the need for further research to identify novel therapeutic targets for this condition.
Oxidative Stress and Antioxidants in Pediatric Asthma’s Evolution and Management
Authors: Ioniuc Ileana Katerina , Lupu Ancuta , Dragan Felicia , Tarnita Irina , Alexoae Monica Mihaela , Streanga Violeta , Mitrofan Costica , Thet Aye Aung , Nedelcu Alin Horatiu , Salaru Delia Lidia , Burlea Stefan Lucian , Mitrofan Elena Cristina , Lupu Vasile Valeriu , Azoicai Alice Nicoleta
Published: October 2024

Within the pediatric population, bronchial asthma is one of the most prevalent chronic respiratory system diseases. The number of exacerbations, severity, and duration of symptoms all have a significant impact on children’s life quality. In the last decades, the prevention and management strategies of this pathology have focused on maintaining or even increasing the pulmonary function to maximum levels in early childhood, as it has been demonstrated that functional deficits at this level occurring before school age cause pathological manifestations later, in adulthood. The epithelium of the airways and implicitly that of the lung is the first barrier against the lesions caused by pro-oxidative factors. Both oxidative and antioxidative factors can be of endogenous origin (produced by the body) or exogenous (from the environment or diet). Good functioning of antioxidant defense mechanisms from the molecular level to the tissue level, and a balance between pro-oxidative factors and anti- oxidative factors, influence the occurrence of compensatory mechanisms at the level of the respiratory epithelium, causing the delay of local responses to the stress induced by chronic inflammation (bronchial remodeling, thickening of airway smooth muscles, bronchoconstriction, bronchial hyper-reactivity). These mechanisms underlie the pathophysiological changes in asthma. Numerous studies carried out among the pediatric population inclusively have demonstrated the effectiveness of antioxidants in the prophylaxis, slowing down and preventing the progression of this pathology. This review complements the scientific articles, aiming at emphasizing the complexity of oxidative physio-pathological pathways and their importance in the occurrence, development, and therapeutic response in asthma, providing a good understanding of the relationship between oxidative and antioxidative factors, and being a source of future therapeutic strategies.
An Experimental Study on the Effect of GFRP and CFRP Strengthening on the Static and Dynamic Behavior of R/C Beams Under Progressive Damage
Authors: Ozturkoglu Onur , Yucel Umut , Karademir Cihan , Durmazgezer Erkan
Published: October 2024

This paper aims to investigate the effect of glass fiber-reinforced polymer (GFRP) and carbon fiber-reinforced polymer (CFRP) strengthening materials on the static and dynamic behavior of reinforced concrete (R/C) beams subjected to progressive damage. Four identical beams, each strengthened with either GFRP or CFRP, are tested under a cyclic quasi-static loading pattern. Impact hammer tests are performed for undamaged states and various damage levels of the beams. The dynamic test data are analyzed using the Enhanced Frequency Domain Decomposition (EFDD) method to estimate the dynamic characteristics of the beams. In this context, the first three vibration modes in both vertical and horizontal directions are considered. Strengthening is applied to both pre-damaged and undamaged beams, enabling a comparison of their performance before and after the strengthening procedure. Beams strengthened with CFRP exhibit a higher load-bearing capacity and stiffness but also fail at lower displacement levels compared to those strengthened with GFRP, which demonstrate more ductile behavior. Furthermore, the modal frequency ratios indicate that the first vibration mode is more sensitive to damage than the second and third modes. This study highlights the effectiveness of both strengthening materials in enhancing the structural performance of both undamaged and damaged beams.
A Short Review on the Wire-Based Directed Energy Deposition of Metals: Mechanical and Microstructural Properties and Quality Enhancement
Authors: Ghasempour-Mouziraji Mehran , Afonso Daniel , Sousa Ricardo Alves de
Published: October 2024

Wire-based directed energy deposition (WDED) is an emerging additive manufacturing process garnering significant attention due to its potential for fabricating metal components with tailored mechanical and microstructural properties. This study reviews the WDED process, focusing on fabrication techniques, mechanical behaviors, microstructural characteristics, and quality enhancement methods. Utilizing data from the Web of Science, the study identifies leading countries in WDED research and highlights a growing interest in the field, particularly in materials engineering. Stainless steel, titanium, aluminum, and copper-based alloys are prominent materials for WDED applications. Furthermore, the study explores post-processing techniques such as machining, heat treatment, and surface finishing as integral steps for quality enhancement in WDED components.
CFD Evaluation of Respiratory Particle Dispersion and Associated Infection Risk in a Coach Bus with Different Ventilation Configurations
Authors: Scungio Mauro , Parlani Giulia , Buonanno Giorgio , Stabile Luca
Published: October 2024

The COVID-19 pandemic has underscored the urgency of understanding virus transmission dynamics, particularly in indoor environments characterized by high occupancy and suboptimal ventilation systems. Airborne transmission, recognized by the World Health Organization (WHO), poses a significant risk, influenced by various factors, including contact duration, individual susceptibility, and environmental conditions. Respiratory particles play a pivotal role in viral spread, remaining suspended in the air for varying durations and distances. Experimental studies provide insights into particle dispersion characteristics, especially in indoor environments where ventilation systems may be inadequate. However, experimental challenges necessitate complementary numerical modeling approaches. Zero-dimensional models offer simplified estimations but lack spatial and temporal resolution, whereas Computational Fluid Dynamics, particularly with the Discrete Phase Model, overcomes these limitations by simulating airflow and particle dispersion comprehensively. This paper employs CFD-DPM to simulate airflow and particle dispersion in a coach bus, offering insights into virus transmission dynamics. This study evaluates the COVID-19 risk of infection for vulnerable individuals sharing space with an infected passenger and investigates the efficacy of personal ventilation in reducing infection risk. Indeed, the CFD simulations revealed the crucial role of ventilation systems in reducing COVID-19 transmission risk within coach buses: increasing clean airflow rate and implementing personal ventilation significantly decreased particle concentration. Overall, infection risk was negligible for scenarios involving only breathing but significant for prolonged exposure to a speaking infected individual. The findings contribute to understanding infection risk in public transportation, emphasizing the need for optimal ventilation strategies to ensure passenger safety and mitigate virus transmission.
The Effectiveness of Applying Artificial Intelligence in Sick Children’s Communication
Authors: Huang Hsin-Shu , Lee Bih-O
Published: October 2024

Pediatric nursing students are required to be taught how to overcome their own psychological stress during their internship in order to understand sick children’s emotional reactions, as well as to be able to interact and communicate with such children. This quasi-experimental study proves that the application of AI image health education e-books by the nursing teacher is more effective than traditional paper handout teaching materials in improving nursing students’ self-efficacy when using therapeutic games to deal with and reduce sick children’s fears of medical examinations and treatments (p < 0.05). AI-driven tools can enable the development of personalized e-learning materials that target specific areas for cognitive improvement. This targeted approach can enhance knowledge retention and skill development, resulting in better-prepared healthcare professionals.
The Pivotal Role of One-Carbon Metabolism in Neoplastic Progression During the Aging Process
Authors: Majumder Avisek , Bano Shabana , Nayak Kasturi Bala
Published: October 2024

One-carbon (1C) metabolism is a complex network of metabolic reactions closely related to producing 1C units (as methyl groups) and utilizing them for different anabolic processes, including nucleotide synthesis, methylation, protein synthesis, and reductive metabolism. These pathways support the high proliferative rate of cancer cells. While drugs that target 1C metabolism (like methotrexate) have been used for cancer treatment, they often have significant side effects. Therefore, developing new drugs with minimal side effects is necessary for effective cancer treatment. Methionine, glycine, and serine are the main three precursors of 1C metabolism. One-carbon metabolism is vital not only for proliferative cells but also for non-proliferative cells in regulating energy homeostasis and the aging process. Understanding the potential role of 1C metabolism in aging is crucial for advancing our knowledge of neoplastic progression. This review provides a comprehensive understanding of the molecular complexities of 1C metabolism in the context of cancer and aging, paving the way for researchers to explore new avenues for developing advanced therapeutic interventions for cancer.
Beyond Wolfram Syndrome 1: The WFS1 Gene’s Role in Alzheimer’s Disease and Sleep Disorders
Authors: Caruso Valerio , Rigoli Luciana
Published: October 2024

The WFS1 gene was first identified in Wolfram Syndrome 1 (WS1), a rare autosomal recessive genetic disorder characterized by severe and progressive neurodegenerative changes. WFS1’s role in various cellular mechanisms, particularly in calcium homeostasis and the modulation of endoplasmic reticulum (ER) stress, suggests its potential involvement in the pathogenesis of Alzheimer’s disease (AD) and sleep disorders. Because it is involved in maintaining ER balance, calcium signaling, and stress responses, WFS1 plays a multifaceted role in neuronal health. Numerous studies have shown that the absence or improper expression of WFS1 disrupts these cellular processes, leading to neurodegeneration and making neurons more vulnerable. In AD, WFS1 dysfunction is thought to contribute to the accumulation of amyloid-β (Aβ) plaques and tau tangles, thereby accelerating disease progression. Additionally, WFS1 plays an essential role in sleep regulation by influencing neuronal excitability and neurotransmitter release, which may explain the sleep disturbances frequently observed in neurodegenerative diseases. Due to its involvement in the pathological mechanisms of AD and sleep disorders, WFS1 is regarded as a potential early diagnostic marker for these diseases. Further research is required to fully elucidate WFS1’s role in the cellular pathway, perhaps facilitating the development of new therapeutic strategies for Alzheimer’s disease and sleep disorders.
Cerebrolysin in Patients Diagnosed with Subarachnoid Hemorrhage—The Results of an Observational Cohort Study
Authors: Kojder Klaudyna , Jarosz Konrad , Andrzejewska Agata , Solek-Pastuszka Joanna , Skonieczna-Żydecka Karolina , Kaczmarczyk Mariusz , Jurczak Anna
Published: October 2024

Background: Subarachnoid hemorrhage (SAH) is associated with high mortality and a high level of disability. Progress in surgical and endovascular techniques has lowered the mortality rate in patients with SAH. However, many patients are left with neurological impairment. Objectives: In our study we wanted to examine the impact of Cerebrolysin on treatment results in patients with SAH diagnosis. Methods: The data of 47 patients, divided into Cerebrolysin (26) and non-Cerebrolysin (21) group were included. We examined the correlation between Cerebrolysin administration and additional Amantadine treatment or neuromonitoring, craniectomy, and endovascular treatment and its impact on the Glasgow Outcome Scale (GOS) score, length of stay (LOS), and mortality. Results: Our study shows that Cerebrolysin improves the mortality rate in combination with neuromonitoring in a group of patients with severe SAH. It does not affect the raw values of GOS or LOS in patients with SAH. Conclusions: Further studies with larger patient groups are needed to investigate the role of Cerebrolysin as an additional treatment in SAH.
MiRNA Expression in Long-Distance Runners with Musculoskeletal Pain: Implications for Pain Pathophysiology
Authors: Tumolo Maria Rosaria , Bodini Antonella , Bagordo Francesco , Leo Carlo Giacomo , Mincarone Pierpaolo , De Matteis Elisabetta , Sabina Saverio , Grassi Tiziana , Scoditti Egeria
Published: October 2024

Background: miRNAs are short, non-coding RNAs whose deregulation has been shown in painful processes, including musculoskeletal pain. This condition, which causes disability, impacts quality of life, and contributes to substantial healthcare costs, is also a critical issue in sports. In this case-control study, we evaluated the expression of four miRNAs involved in inflammation in runners with musculoskeletal pain and elucidated their functions and pathophysiological implications. Methods: A total of 17 runners with musculoskeletal pain and 17 age- and sex-matched runners without pain participated in this study. The levels of the miRNAs were evaluated by qRT-PCR. Bioinformatic tools were employed to identify the target genes and biological processes regulated by these miRNAs. Results: Compared to the controls, the runners with musculoskeletal pain exhibited significantly higher plasma levels of miR-133b (p = 0.02), miR-155-5p (p = 0.003) and let-7a-5p (p = 0.02). Multivariable regression analysis indicated that these three miRNAs exhibit a positive correlation (p < 0.05) with the presence of musculoskeletal pain, adjusted for age. Bioinformatic analysis suggested that the miRNAs hub genes are involved in regulatory processes, neuroinflammatory pathways, and human diseases that are associated with pain pathology. Conclusions: These results enhance our understanding of the potential role of miR-133b, miR-155-5p and let-7a-5p in pain-associated biological processes. The miRNA-mediated negative regulation of genes identified could explain the inflammatory and tissue repair processes in this population. Further studies are needed to confirm and validate the role of these miRNAs in painful conditions, especially considering the significant public health implications of managing inflammatory pain in sports.
Biomechanical Analysis of Diffuse Idiopathic Skeletal Hyperostosis and Osteoporosis: Vertebral Fracture Risk Evaluation Using Finite Element Modeling with Clinical and Micro-CT Data in an Elderly Donor
Authors: Schröder Guido , Mittlmeier Thomas , Falk Steffi S. I. , Götz Andreas , Kruse Josephine , Akl Estelle , Kröger Hannes , Andresen Julian Ramin , Andresen Reimer , Schober Hans-Christof , Glass Änne
Published: October 2024

Introduction: Biomechanical analysis of spinal structures is crucial in the evaluation of injuries, the risk of fracture, and age-related changes. Osteoporotic vertebrae are very fragile and therefore constitute a serious risk, especially in the elderly. Methods: At present, clinically relevant decision making in fracture risk assessment is predicated upon finite element analysis (FEA), which utilizes high-resolution computed tomography (CT) scans from clinical practice alongside micro-CT scans from laboratory settings. Of particular interest is the utilization of cortical vertebral body thicknesses, as meticulously measured via micro-CT. The data from a body donation over 80 years old with diffuse idiopathic skeletal hyperostosis (DISH) and osteoporosis (OP) were utilized through FEA to evaluate stresses in cortical and trabecular bone and to predict the stiffness and deformability of the examined vertebral bodies. Results: The investigation revealed a higher density of cortical and cancellous bone in vertebrae affected by DISH. Cortical density was highest in the thoracic section (median 188 µm), while cancellous bone density was 222 HU in the cervical vertebrae. The load on cortical bone increased as we progressed towards the lumbar spine; however, it remained quite constant in cancellous bone. Despite a low bone density, we registered no fractures in vertebrae. Conclusions: The data showed that DISH increased the thickness of the cortical bone and the density of the cancellous bone. The combination of increased cortical and cancellous bone density might reduce the risk of fracture in patients with low bone density. These conclusions emphasize the significance of biomechanical properties in the assessment of fracture risk and have important implications for clinical practice, particularly in relation to the prevention of vertebral fractures in osteoporotic patients with DISH.
A Randomized Clinical Study of a Curcumin and Melatonin Toothpaste Against Periodontal Bacteria
Authors: Pulcini Riccardo , Chiarelli Antonio Maria , Sinjari Bruna , Esposito Jessica Elisabetta , Avolio Francesco , Martinotti Riccardo , Pignatelli Vittorio , Pignatelli Luca , Berlincioni Laura , Martinotti Stefano , Toniato Elena
Published: October 2024

Background: The mouth and the oropharyngeal system are home to numerous bacterial species that constitute the so-called oral microbiome and play an important role for the integrity of the oral cavity, influencing the overall health of the body, as demonstrated by several studies. The aim of this study was to evaluate the bacterial modulation potential of a toothpaste (bioredoxin) containing curcumin and melatonin. Both substances have anti-inflammatory properties, as documented in several scientific reports. Methods: The in vivo study we present was a single-center, double-blind trial and was conducted in parallel groups. We enlisted 20 volunteers who were randomly assigned to four distinct groups using blinded four different toothpaste preparations: a standard toothpaste indicated as placebo, a toothpaste with curcumin, a toothpaste with melatonin, and a toothpaste with melatonin and curcumin. Results: The samples from the gingival tasks were taken at time 0 and after 8 weeks of toothpaste treatment. By evaluating the DNA content of the most significant periodontal bacteria related to the total bacteria count using quantitative PCR assays, including the saprophyte component of the microbiome, we demonstrated that the Curcumin and Melatonin treatment has a statistically relevant effect on decreasing the level of periodontal pathogenic bacteria DNA. The toothpaste with the addition of curcumin and melatonin showed a modulation between t0 and t1 of the Campylobacter rectus (14,568 vs. 3532.8) and Peptostreptococcus micro (1320.8 vs. 319) bacteria. In addition, a modulation of pathogenic bacteria and saprophytic bacteria was shown. The synergistic action of the two additives would therefore appear to lead to promising results. Conclusions: Despite the fact that additional studies may be necessary in evaluating the effect of the Curcumin/melatonin combination in modulating a proposed therapeutic effect on infections of the oropharyngeal apparatus, in this report, we show for the first time that a combination of curcumin and melatonin supplemented using an oral cosmetic vehicle has the capacity to decrease the level of periodontal pathogenic bacteria, possibly ameliorating health and the physiological conditions in the buccal scenario.
Plasma Brain-Derived Neurotrophic Factor (BDNF) Levels and BDNF Promoters’ DNA Methylation in Workers Exposed to Occupational Stress and Suffering from Psychiatric Disorders
Authors: Veltri Antonello , Nicolì Vanessa , Marino Riccardo , Rea Filomena , Corsi Martina , Chiumiento Martina , Giangreco Marianna , Caldi Fabrizio , Guglielmi Giovanni , Foddis Rudy , Coppedè Fabio , Silvestri Roberto , Buselli Rodolfo
Published: October 2024

Introduction: Decreased plasma BDNF (pBDNF) levels have been proposed as a biomarker in the illness phases of mood disorders. This cross-sectional study aimed to evaluate the pBDNF and BDNF promoters’ DNA methylation levels in workers exposed to occupational stress and suffering from work-related stress disorders. Methods: the pBDNF and BDNF exon I and IV promoters’ methylation levels were measured by specific immunoassays and methylation-sensitive high-resolution melting (MS-HRM) in 62 patients with adjustment disorders (AD), 79 patients with major depressive disorder (MDD) and 44 healthy controls. Occupational stress was evaluated in the patients and controls using the Job Content Questionnaire (JCQ). Results: the pBDNF levels were significantly higher in the MDD (p < 0.001) and AD (p < 0.0001) patients than in the controls. The MDD patients showed significantly lower pBDNF levels than the AD ones (p = 0.01). The BDNF exon I and IV promoters’ methylation levels were significantly higher in the MDD patients than in the AD ones (exon I promoter: p = 0.0001, exon IV promoter: p < 0.0001) and controls (exon I promoter: p = 0.0001, exon IV promoter: p < 0.0001). In the patients, but not in the controls, the BDNF promoters’ methylation levels showed significant negative correlations with occupational stress. Conclusions: BDNF could play a key role in the pathophysiology of stress-related disorders and the peripheral elevation of it observed in patients exposed to occupational stress could suggest a protective mechanism for neurons from stress-mediated damage. The elevation of the pBDNF levels, even in MDD, may characterize a “reactive” subtype of depressive episode, while the significant elevation of the BDNF promoters’ methylation levels in depressed patients could indicate a predisposition to more severe illness under stress. Further research is needed, focusing on biomarkers for stress-related disorders as a potential tool for the diagnosis and prevention of occupational diseases.
Healing Environment in Pediatric Cancer Centers by Utilizing Positive Distractions
Authors: Alhsainat Aseel , Günçe Kağan
Published: October 2024

In recent years, a broader perspective has viewed the use of therapeutic environments in healthcare, transforming the hospital’s environment into an energizing atmosphere that benefits both employees and patients. Scientists found that exposure to natural elements like nature scenes, sunlight, art, calming colors, and soothing sounds significantly sped up healing and created a more relaxed hospital environment. This research emphasized the importance of healthcare settings’ interior design in promoting patient well-being through beneficial distractions. The motivation for this investigation came from the need to develop an understanding of positive distractions in the cancer care environment and showed how this understanding could be used to enhance the space experience to promote health and well-being. This research adopted a mixed-methods approach. First, a qualitative method using a critical review of the literature was used to effectively present, analyze, and synthesize literature from diverse sources, followed by a quasi-experimental research method. This research is intended to focus on the attributes of positive distraction as one of the psychosocially and psychologically supportive healthcare design aspects that evoke positive feelings in children’s cancer center experiences.
Interdisciplinary Perspectives on Agent-Based Modeling in the Architecture, Engineering, and Construction Industry: A Comprehensive Review
Authors: Mazzetto Silvia
Published: October 2024

This paper explores the transformative impact of agent-based modeling (ABM) on the architecture, engineering, and construction (AEC) industry, highlighting its indispensable role in revolutionizing project management, construction processes, safety protocols, and sustainability initiatives including energy optimization and occupants’ comfort. Through an in-depth review of 178 documents published between 1970 and 2024 on current practices and the integration of ABM with emerging digital technologies, this study underscores the critical importance of ABM in facilitating enhanced decision-making, resource optimization, and complex system simulations. For instance, ABM is shown to reduce project delays by up to 15% through enhanced resource allocation and improve safety outcomes by simulating worker behavior and identifying potential hazards in dynamic construction environments. The results reveal ABM’s potential to significantly improve construction methodologies, integrate technological advancements seamlessly, and contribute to the development of sustainable and resilient building practices. Furthermore, this paper identifies key areas for future research, including the exploration of ABM’s capabilities in conjunction with other digital innovations to unlock new avenues for efficiency and sustainability in construction. This study sets out a forward-looking agenda for providing this modeling approach to address contemporary challenges and harness opportunities for innovation and growth in the AEC sector.
Cyclic Performance of Reinforced Concrete Columns Strengthened with Basalt-Fibre-Reinforced Cementitious Matrix (BFRCM)
Authors: Prasath Chellappandian Rajendra , Jayaguru Chandrasekaran , Arunachelam Nakarajan
Published: October 2024

The purpose of this experimental investigation was to confirm whether the Basalt-Fibre-Reinforced Cement Matrix (BFRCM) effectively enhances the cyclic performance of columns made of reinforced concrete (RC). The BFRCM system, which comprises basalt fabric mesh reinforced with a cementitious matrix containing polyvinyl alcohol (PVA) fibre, has significant practical implications. In the testing phase, concrete and steel reinforcement were used to create RC column specimens, which were then strengthened with three, four, and five layers of BFRCM. The horizontal cyclic and constant axial loads were applied and tested to evaluate the performance of RC columns with and without strengthening. By improving the energy dissipation by approximately 9 to 32% and increasing stiffness by roughly 24 to 44%, the column specimen with three, four, and five layers of BFRCM performed better than the control specimen. Furthermore, incorporating short fibre into the matrix effectively improved the tensile properties of the FRCM systems and decreased the shrinkage-induced cracks. The increased stiffness indicates that the column with BFRCM has better structural strength because it can sustain higher loads with less deflection. The thorough comparative analyses examined the RC column specimens’ failure modes, hysteretic responses, stiffness degradation, and energy dissipation, providing a reliable basis for the conclusions. The test results confirmed that the BFRCM effectively enhanced the seismic capabilities and has been promised a way to strengthen RC elements, providing valuable insights for civil engineering and materials science.
Fabrication of Cu-Doped Diamond-like Carbon Film for Improving Sealing Performance of Hydraulic Cylinder of Shearers
Authors: Yang Yanrong , Yu Xiang , Zhao Zhiyan , Zhang Lei
Published: October 2024

During shearer operation, the piston rod is susceptible to wear from the invasion of pollutants, thus ruining the sealing ring in the hydraulic cylinder. This work attempts to conduct a systematic investigation of Cu-doped diamond-like carbon (Cu-DLC) film to improve the seal performance. The failure process of the cylinder was analyzed, and relevant parameters were determined. Several Cu-DLC films were deposited on the substrate of the piston rod in a multi-ion beam-assisted system, and their structures and combined tribological performances were investigated. The hardness of the film ranges from 27.6 GPa to 14.8 GPa, and the internal stress ranges from 3500 MPa to 1750 MPa. The steady-state frictional coefficient of the film ranges from 0.04 to 0.15; the wear rate decreases first and then increases, and it reaches its lowest (5.0 × 10−9 mm3/N·m) at 9.2 at.% content. a:C-Cu9.2% film presents optimal combined tribological performances in this experiment. The modification mechanism of Cu-DLC film for the seal performance may come from the synergistic effects of (i) the contact force and friction-heat-induced film graphitization, (ii) Cu doping improves the toughness of the film and acts as a solid lubricant, and (iii) the transfer layer plays a role in self-lubrication.
Automated Organ Segmentation for Radiation Therapy: A Comparative Analysis of AI-Based Tools Versus Manual Contouring in Korean Cancer Patients
Authors: Choi Seo Hee , Park Jong Won , Cho Yeona , Yang Gowoon , Yoon Hong In
Published: October 2024

Background: Accurate delineation of tumors and organs at risk (OARs) is crucial for intensity-modulated radiation therapy. This study aimed to evaluate the performance of OncoStudio, an AI-based auto-segmentation tool developed for Korean patients, compared with Protégé AI, a globally developed tool that uses data from Korean cancer patients. Methods: A retrospective analysis of 1200 Korean cancer patients treated with radiotherapy was conducted. Auto-contours generated via OncoStudio and Protégé AI were compared with manual contours across the head and neck and thoracic, abdominal, and pelvic organs. Accuracy was assessed using the Dice similarity coefficient (DSC), mean surface distance (MSD), and 95% Hausdorff distance (HD). Feedback was obtained from 10 participants, including radiation oncologists, residents, and radiation therapists, via an online survey with a Turing test component. Results: OncoStudio outperformed Protégé AI in 85% of the evaluated OARs (p < 0.001). For head and neck organs, OncoStudio achieved a similar DSC (0.70 vs. 0.70, p = 0.637) but significantly lower MSD and 95% HD values (p < 0.001). In thoracic organs, OncoStudio performed excellently in 90% of cases, with a significantly greater DSC (male: 0.87 vs. 0.82, p < 0.001; female: 0.95 vs. 0.87, p < 0.001). OncoStudio also demonstrated superior accuracy in abdominal (DSC 0.88 vs. 0.81, p < 0.001) and pelvic organs (male: DSC 0.95 vs. 0.85, p < 0.001; female: DSC 0.82 vs. 0.73, p < 0.001). Clinicians favored OncoStudio in 70% of cases, with 90% endorsing its clinical suitability for Korean patients. Conclusions: OncoStudio, which is tailored for Korean patients, demonstrated superior segmentation accuracy across multiple anatomical regions, suggesting its suitability for radiotherapy planning in this population.
Enhancing Surgical Guidance: Deep Learning-Based Liver Vessel Segmentation in Real-Time Ultrasound Video Frames
Authors: Awais Muhammad , Al Taie Mais , O’Connor Caleb S. , Castelo Austin H. , Acidi Belkacem , Tran Cao Hop S. , Brock Kristy K.
Published: October 2024

Background/Objectives: In the field of surgical medicine, the planning and execution of liver resection procedures present formidable challenges, primarily attributable to the intricate and highly individualized nature of liver vascular anatomy. In the current surgical milieu, intraoperative ultrasonography (IOUS) has become indispensable; however, traditional 2D ultrasound imaging’s interpretability is hindered by noise and speckle artifacts. Accurate identification of critical structures for preservation during hepatectomy requires advanced surgical skills. Methods: An AI-based model that can help detect and recognize vessels including the inferior vena cava (IVC); the right (RHV), middle (MHV), and left (LVH) hepatic veins; the portal vein (PV) and its major first and second order branches the left portal vein (LPV), right portal vein (RPV), and right anterior (RAPV) and posterior (RPPV) portal veins, for real-time IOUS navigation can be of immense value in liver surgery. This research aims to advance the capabilities of IOUS-guided interventions by applying an innovative AI-based approach named the “2D-weigthed U-Net model” for the segmentation of multiple blood vessels in real-time IOUS video frames. Results: Our proposed deep learning (DL) model achieved a mean Dice score of 0.92 for IVC, 0.90 for RHV, 0.89 for MHV, 0.86 for LHV, 0.95 for PV, 0.93 for LPV, 0.84 for RPV, 0.85 for RAPV, and 0.96 for RPPV. Conclusion: In the future, this research will be extended for real-time multi-label segmentation of extended vasculature in the liver, followed by the translation of our model into the surgical suite.
Characteristics of Cancer in Subjects Carrying Lynch Syndrome-Associated Gene Variants in Taiwanese Population: A Hospital-Based Study in Taiwan
Authors: Chen Yi-Peng , Hsiao Tzu-Hung , Lin Wan-Tzu , Liao Yi-Jun , Liao Szu-Chia , Tsai Hsin-Ju , Chen Yen-Ju , Jhan Pei-Pei , Kao Pei-Ying , Lin Ying-Cheng , Chuang Han-Ni
Published: October 2024

Lynch syndrome (LS) is an autosomal dominant disorder characterized by increased risks of colorectal and endometrial cancers. LS is defined by pathogenic variants in mismatch repair (MMR) genes, including MLH1, MSH2, and MSH6. Data on the prevalence and associated cancer risks of LS in the Han Chinese population remain limited. In this study, using a broad biobank approach through the Taiwan Precision Medicine Initiative (TPMI), we identified LS-associated MMR gene variants within a cohort of 42,828 participants from a Taiwanese medical center. A total of 89 individuals were found to carry pathogenic MMR variants: MLH1 (n = 22, 25%), MSH2 (n = 47, 53%), and MSH6 (n = 20, 22%). The overall prevalence of MMR variants was calculated, and cancer incidence rates among carriers were determined. The prevalence of MMR variants in the study population was 1 in 481. The distribution of MLH1, MSH2, and MSH6 variants were 24.7%, 52.8%, and 22.5%, respectively. Cumulative cancer incidence rates of carriers were 40.9% for MLH1 carriers, 29.8% for MSH2, and 40% for MSH6. Among the 19 individuals who underwent colonoscopy screening, the prevalence of polyps was similar to that of the control group (adenoma detection rate: 32% vs 26%, p = 0.585). A meticulous analysis of the detected polyps in seven participants, considering factors such as location, size, morphology, and pathological features, showed no significant differences from controls. A significant cancer risk is associated with LS-related MMR variants in the Taiwanese population. The apparent under diagnosis of LS highlights the urgent need for enhanced surveillance and genetic counseling in this demographic. Our findings suggest that adjustments in the current screening protocols may be warranted to better identify and manage at-risk individuals.
Integrin α6β4 Upregulates PTPRZ1 Through UCHL1-Mediated Hif-1α Nuclear Accumulation to Promote Triple-Negative Breast Cancer Cell Invasive Properties
Authors: Chen Min , Karimpour Parvanee A. , Elliott Andrew , He Daheng , Knifley Teresa , Liu Jinpeng , Wang Chi , O’Connor Kathleen L.
Published: October 2024

Integrin α6β4 drives triple-negative breast cancer (TNBC) aggressiveness through the transcriptional regulation of key genes. Here, we investigated how integrin α6β4 regulates protein tyrosine phosphatase receptor type Z1 (PTPRZ1). Using stable re-expression of integrin β4 (ITGB4) in cells naturally devoid of integrin α6β4 or knockdown or knockout (KO) of ITGB4, we found that integrin α6β4 regulates PTPRZ1 expression. To gain mechanistic insight, we focused on Hif-1α due to the impact of integrin α6β4 on a hypoxia-associated signature. We found that nuclear localization of Hif-1α, but not Hif-2α, was substantially enhanced with integrin α6β4 signaling. Hif-1α knockdown by shRNA or chemical inhibition decreased PTPRZ1 expression, while chemical activation of Hif-1α increased it. Upstream of Hif-1α, integrin α6β4 upregulates UCHL1 to stabilize Hif-1α and ultimately regulate PTPRZ1. Inhibition of UCHL1 and PTPRZ1 dramatically decreases integrin α6β4-mediated cell migration and three-dimensional invasive growth. Finally, public breast cancer database analyses demonstrated that ITGB4 correlates with PTPRZ1 and that high expression of ITGB4, UCHL1, HIF1A, and PTPRZ1 associated with decreased overall survival, distant metastasis free survival, post progression survival, and relapse-free survival. In summary, these findings provide a novel function of integrin α6β4 in promoting tumor invasive phenotypes through UCHL1-Hif-1α-mediated regulation of PTPRZ1.
Copper and Colorectal Cancer
Authors: Małyszko Maciej , Przybyłkowski Adam
Published: October 2024

Minerals constitute only 5% of the typical human diet but are vital for health and functionality. Copper, a trace element, is absorbed by the human gut at 30–40% from diets typical of industrialized countries. The liver produces metallothioneins, which store copper. Copper is crucial for mitochondrial respiration, pigmentation, iron transport, antioxidant defense, hormone production, and extracellular matrix biosynthesis. Copper deficiency, often caused by mutations in the ATP7A gene, results in Menkes disease, an X-linked recessive disorder. On the contrary, Wilson disease is characterized by toxic copper accumulation. Cuproptosis, a unique form of cell death regulated by copper, is a subtype of necrosis induced by enhanced mitochondrial metabolism and intracellular copper accumulation. This process can reduce the malignant potential of tumor cells by inhibiting glucose metabolism. Therapeutically, copper and its complexes have shown efficacy in malignancy treatments. The disruption of copper homeostasis and excessive cuproplasia are significant in colorectal cancer development and metastasis. Therefore, manipulating copper status presents a potential therapeutic target for colorectal cancer, using copper chelators to inhibit copper formation or copper ion carriers to promote cuproptosis. This review highlights the role of copper in human physiology and pathology, emphasizing its impact on colorectal cancer and potential therapeutic strategies. Future AI-based approaches are anticipated to accelerate the development of new compounds targeting cuproptosis and copper disruption in colorectal cancer.
Performance Monitoring of Greenhouse Biogas Digester
Authors: Mzobotshe Mandilakhe , Mukumba Patrick , Tangwe Stephen , Lethole Ndaduleni , Obileke KeChrist
Published: October 2024

The country of South Africa is facing an energy crisis due to heavy reliance on fossil fuels, resulting in continuous load shedding. The use of renewable energy technologies can help resolve the current electricity crisis in the country. Moreover, waste-to-energy conversion has the potential to greatly contribute to economic development and improve public health. One such technology is biomass, which exploits waste-to-energy conversion. Additionally, solar energy can be utilized to maintain appropriate digester temperatures for optimal biogas yield. The study aims to assemble a portable balloon biogas digester in an enclosed greenhouse cavity and feed it with cow dung. Daily monitoring of pH and temperature (ambient, greenhouse, and slurry) was conducted, while biogas yield was monitored using a serial residential diaphragm flow meter. Furthermore, the composition of methane was monitored using the SAZQ biogas analyzer. The study investigated the impact of temperature on biogas production. The results revealed that the gas production rate of biogas fermentation increased within a certain temperature range. Therefore, maximum biogas production was achieved at a pH of 6.84 to 7.03, and the composition of methane exceeded 50%. Consequently, the study concluded by indicating that the digester housed within a greenhouse envelope, as demonstrated in this novel study, maintains the temperature within the optimal mesophilic range necessary for anaerobic digestion.
The Children’s Somatic Symptoms Inventory-8: Psychometric Properties of a Brief Measure of Somatic Distress
Authors: Stone Amanda L. , Garber Judy , Walker Lynn S.
Published: October 2024

Background: Children often present to primary and specialty care clinics with multiple somatic symptoms of nonspecific origin that can be highly distressing and prompt significant health service use. We evaluated the psychometric properties of the eight-item Children’s Somatic Symptoms Inventory (CSSI-8) as a brief measure of somatic distress that could be easily integrated into clinical systems. Method: Eight items from the 24-item CSSI were selected based on their representation of multiple bodily systems, association with high base rates, and ability to maximize the separation of the items’ Rasch measure scores. The psychometric quality of the eight-item scale was evaluated in 876 pediatric patients with chronic abdominal pain and a nonclinical sample of 954 school children using methods from three psychometric models (the classical test theory, Rasch modeling, and confirmatory factor analysis). Results: The CSSI-8 showed good measurement properties on an extensive array of psychometric criteria, had adequate Rasch person separation reliability for a brief instrument (rsep = 0.74–0.75), and distinguished between clinical and nonclinical youth. Girls in both groups had significantly higher CSSI-8 scores than boys. Norms for the clinical sample are presented. Conclusions: The CSSI-8 is a psychometrically sound measure suitable for use as a brief dimensional assessment of pediatric somatic distress.
Can AI Robot Teaching Improve Children’s Performance in Housework? A Quasi-Experimental Study
Authors: Lee Ching-Fen , Tang Shain-May
Published: October 2024

Background: The primary objective of this study is to explore the effects of using Artificial Intelligence (AI) robot-assisted instruction in preschool activities on young children’s performance in housework. Methods: A quasi-experimental design was employed, with an experimental group and two control groups, to observe changes in children’s performance in housework before and after AI robot-assisted teaching. The study sample consisted of preschoolers from metropolitan Taipei, with 65 children in the experimental group, 53 in control group 1, and 75 in control group 2. Children participating in the research project required consent from the school, teachers, and parents. The children in the experimental group received AI robot-assisted instruction on household tasks for two days a week over three consecutive weeks, with each session lasting approximately 20–30 min. Control group 1 did not receive any experimental treatment, while control group 2 underwent the same learning schedule and content as the experimental group but was instructed by teachers instead of AI robots. The data were analyzed based on pre- and post-test surveys of parents observing their children’s performance in household tasks. Results: This study found that both AI robot-assisted teaching and teacher-led instruction enhanced children’s household skills, with the AI robot-assisted method showing slightly better results. These findings suggest that both technological tools and teacher guidance can effectively improve children’s housework performance, while artificial intelligence robots may provide young children with motivation and curiosity to learn due to their appearance and interactive design, which deserves further analysis. Conclusion: As technology rapidly advances, young children are exposed to various technological devices at an early age. Integrating technological media into future preschool teaching is inevitable, and leveraging tools like AI robots to support teaching, reduce teacher burden, and diversify instructional methods could be a direction worth considering.