bluuebunny/crossref_metadata_2025 huggingface dataset:
Deconstructing Global Observed and Reanalysis Total Cloud Cover Fields Based on Pacific Climate Modes
Authors: Vaideanu Petru , Ionita Monica , Voiculescu Mirela , Rimbu Norel
Published: February 2023

Clouds are notoriously difficult to simulate. Here, we separate and quantify the impact of Pacific climate modes on total cloud cover (TCC) variability, using reliable satellite observations together with state-of-the art reanalysis outputs, over the 1979–2020 period. The two most prominent modes of annual TCC variability show intense loadings over the Pacific basin and explain most of the variance in what could be considered the “signal” in satellite TCC data. Canonical correlation analysis (CCA) provides coupled TCC—sea surface temperature (SST) patterns that are linked to the Eastern Pacific (EP) ElNiño—Southern Oscillation (ENSO) and the Central Pacific (CP) ENSO in a physically consistent manner. The two ENSO modes dominate global coupled SST–TCC variability with the footprint of the CP ENSO explaining roughly half of the variance induced by the EP ENSO among these coupled fields. Both the EP and the CP ENSO exert an influence on Pacific decadal TCC variability. The impact of both ENSO modes on global total cloud cover variability is amplified by two positive feedbacks. These results could be used as a reference for model investigations on future projections of coupled TCC—SST variability responses to the CP and the EP ENSO.
Telluric Currents Generated by Solar Flare Radiation: Physical Model and Numerical Estimations
Authors: Sorokin Valery , Yaschenko Alexey , Mushkarev Georgy , Novikov Victor
Published: February 2023

The current studies of solar-terrestrial relations and possible impact of space weather on the seismic activity are based on statistical analysis without detailed consideration of possible physical mechanism that results in fuzzy and contradictory conclusions. We propose to consider a hypothesis of electromagnetic earthquake triggering by a sharp rise of telluric currents in lithosphere including crust faults due to interaction of solar flare X-ray radiation with ionosphere-atmosphere-lithosphere system resulted in a rise of telluric currents in the crust faults. This hypothesis is based on field and laboratory experiments carried out in Russia within the last forty years and clearly demonstrated a possibility of earthquake triggering by electric current injected into the fault. We developed a mathematical model and computer code for numerical estimations of telluric currents generated by solar flare radiations. The obtained numerical results demonstrate that solar flares can cause variations in the density of telluric currents in the crust faults, comparable to the current densities generated in the Earth’s crust by artificial pulsed power sources capable to trigger earthquakes. Consequently, the triggering of seismic events is possible not only by artificial sources of electric current, but also by ionospheric disturbances caused by strong solar flares.
ENSO and PDO Effect on Stratospheric Dynamics in Isca Numerical Experiments
Authors: Sobaeva Daria , Zyulyaeva Yulia , Gulev Sergey
Published: February 2023

The deterministic forecast of the stratospheric polar vortex intensity (iSPV) is limited by 2 weeks, but it can be probabilistically predicted for a longer period due to low-frequency components of the climate system, such as large-scale sea surface temperature anomalies (SSTAs) (e.g., El Niño Southern Oscillation (ENSO) and Pacific Decadal Oscillation (PDO)). For a qualitative and quantitative assessment of the effect of the large-scale Pacific SSTAs on the iSPV anomalies formation, idealized model experiments were carried out using the Isca platform. There is no statistically significant response of the SPV dynamics to the SSTAs corresponding to PDO phases, but they noticeably correct the effect of ENSO modes when added to it. The effect of the El Niño (EN) and La Niña (LN) events with neutral PDO phases on the iSPV is asymmetric; in the “single” EN experiment the vortex is 40% weaker relative to the control values, and, in the “single” LN, the SPV is weakened by no more than 20%. When EN accompanied with the positive PDO phase, iSPV is reduced by 58%. When the negative PDO phase is added, the EN effect is significantly weakened. The LN effect is weakened by both positive and negative PDO phases.
A Comparison of the Tourist Potential of the Climates of the Coastal Resort at Odesa and the Inland Resort by Lake Svityaz
Authors: Shevchenko Olga , Snizhko Sergiy , Gryniuk Oleg , Matzarakis Andreas
Published: February 2023

The aim of this study is to evaluate and compare the human-biometeorological conditions at two resorts in Ukraine: a coastal resort located at Odesa in southern Ukraine and an inland resort situated by the lake at Svityaz situated in northwest Ukraine. The results of this study can facilitate the assessment of the tourist potentials of both locations by the tourism industry, tour operators, and tourists. The evaluation is based on an analysis of the Physiologically Equivalent Temperature (PET) and parameters presented through the Climate–Tourism/Transfer–Information Scheme (CTIS) for the period 1991–2020. The CTIS data reveal that better conditions in terms of thermal comfort can be found during the warm period from May to September at both sites. The results show that the highest frequency of all grades of heat stress are observed in the last 10-day period of July and in the first 10-day period of August at both stations, but at Odesa, the frequency of heat stress of any grade is approximately 10% higher than at Svityaz. The frequency of moderate, strong and extreme heat stress during the daytime in July and in the first two 10-day periods of August at Odesa ranged from 51.3% to 66.5%, and at Svityaz it ranged between 40.2 and 54.6%. Human-biometeorological conditions during heat waves are more strenuous at Odesa. The frequency of days with extreme heat stress at 12 UTC during summer heat waves is 48.4% at Odesa and 35.6% at Svityaz. The results show a higher frequency of thermal stress at Odesa, which makes this resort less comfortable for people vulnerable to heat stress.
Characteristic Features of the Antarctic Surface Air Temperature with Different Reanalyses and In Situ Observations and Their Uncertainties
Authors: Xin Meijiao , Li Xichen , Zhu Jiang , Song Chentao , Zhou Yi , Wang Wenzhu , Hou Yurong
Published: February 2023

Antarctic surface air temperature (SAT) variability is characterized by strong seasonality and regionality, which are associated with the tropical–polar teleconnections and the radiative forcing caused by the concentration changes in ozone and other greenhouse gases. Nevertheless, the sparse in situ observations and the strong disagreement between different reanalysis datasets hinder coherent conclusions about Antarctic SAT variability. In this study, we use a newly developed statistical method, combined maximum covariance analysis (CMCA), to retrieve coherent SAT modes from six reanalysis datasets and 26 station observations. The results show that the Antarctic SAT variability may be dominated by a continental-wide warming/cooling mode, an East–West Antarctic seesaw mode, and a dipole SAT mode around West Antarctica. These SAT modes are strongly associated with three principal modes of Antarctic atmospheric circulation. Furthermore, all six reanalyses can represent these SAT modes well, compared with the observations, despite a clear deviation over the Antarctic Peninsula associated with the biases in the Foehn wind, which may not be clearly reproduced in a low-resolution reanalysis. This study provides an effective means by which to extract coherent signals from all reanalyses and observations to represent the Antarctic SAT variability, and to improve its predictability and projection.
Picturing the Growth Order of Solutions in Complex Linear Differential–Difference Equations with Coefficients of φ-Order
Authors: Sánchez-Ruiz Luis M. , Datta Sanjib Kumar , Biswas Nityagopal , Legua Matilde
Published: February 2023

Given an unbounded non-decreasing positive function φ, we studied what the relations are between the growth order of any solution of a complex linear differential–difference equation whose coefficients are entire or meromorphic functions of finite φ-order. Our findings extend some earlier well-known results.
Asymptotic Solution of a Singularly Perturbed Integro-Differential Equation with Exponential Inhomogeneity
Authors: Kalimbetov Burkhan , Safonov Valeriy , Zhaidakbayeva Dinara
Published: February 2023

The integro-differential Cauchy problem with exponential inhomogeneity and with a spectral value that turns zero at an isolated point of the segment of the independent variable is considered. The problem belongs to the class of singularly perturbed equations with an unstable spectrum and has not been considered before in the presence of an integral operator. A particular difficulty is its investigation in the neighborhood of the zero spectral value of inhomogeneity. Here, it is not possible to apply the well-known procedure of Lomov’s regularization method, so the authors have chosen the method of constructing the asymptotic solution of the initial problem based on the use of the regularized asymptotic solution of the fundamental solution of the corresponding homogeneous equation whose construction from the positions of the regularization method has not been considered so far. In the case of an unstable spectrum, it is necessary to take into account its point features. In this case, inhomogeneity plays an essential role. It significantly affects the type of singularities in the solution of the initial problem. The fundamental solution allows us to construct asymptotics regardless of the nature of the inhomogeneity (it can be both slowly changing and rapidly changing, for example, rapidly oscillating). The approach developed in the paper is universal with respect to arbitrary inhomogeneity. The first part of the study develops an algorithm for the regularization method to construct the asymptotic (of any order on the parameter) of the fundamental solution of the corresponding homogeneous integro-differential equation. The second part is devoted to constructing the asymptotics of the solution of the original problem. The main asymptotic term is constructed in detail, and the possibility of constructing its higher terms is pointed out. In the case of a stable spectrum, we can construct regularized asymptotics without using a fundamental solution.
Gradients of Variation in the At-Vessel Mortality Rate between Twelve Species of Sharks and Skates Sampled through a Fishery-Independent Trawl Survey in the Asinara Gulf (NW Mediterranean Sea)
Authors: Scacco Umberto , Fortibuoni Tomaso , Baini Matteo , Franceschini Gianluca , Giani Dario , Concato Margherita , Panti Cristina , Izzi Alessia , Angiolillo Michela
Published: February 2023

Elasmobranchs are priority species for conservation due to their rapid decline determined by the unbalanced struggle between a fragile bio-ecology and strong anthropogenic impacts, such as bycatch from professional fishing. In this context, measuring species resistance to catch of poorly selective gear is of paramount importance. During June–October 2022, five experimental fishing campaigns were carried out in the Asinara Gulf (northern Sardinia) through 35 geographically and bathymetrically representative hauls of an area between 30 and 600 m in depth. Skates prevailed over sharks in the number of species, with seven and five species, respectively. We first evaluated the status of each individual with respect to stress due to the trawl’s catch using a three-graded scale. We also recorded individual biometrics (total and disk length, weight and sex, and maturity for males) on board by implementing the best practices in manipulating individuals for physiological recovery and release at sea. After capture, skates resulted in generally better conditions than sharks, although deepwater species of both groups exhibited a worse state than coastal species. The estimated vitality rates also depended on the size of the individuals. This work provides standardized data on the intermingled effect of size, species type, and inhabited depth on the resistance response of some elasmobranch species against capture by trawl fishery activities.
The NGF R100W Mutation, Associated with Hereditary Sensory Autonomic Neuropathy Type V, Specifically Affects the Binding Energetic Landscapes of NGF and of Its Precursor proNGF and p75NTR
Authors: Covaceuszach Sonia , Lamba Doriano
Published: February 2023

Nerve Growth Factor (NGF), the prototype of the neurotrophin family, stimulates morphological differentiation and regulates neuronal gene expression by binding to TrkA and p75NTR receptors. It plays a critical role in maintaining the function and phenotype of peripheral sensory and sympathetic neurons and in mediating pain transmission and perception during adulthood. A point mutation in the NGFB gene (leading to the amino acid substitution R100W) is responsible for Hereditary Sensory and Autonomic Neuropathy type V (HSAN V), leading to a congenital pain insensitivity with no clear cognitive impairments, but with alterations in the NGF/proNGF balance. The available crystal structures of the p75NTR/NGF and 2p75NTR/proNGF complexes offer a starting point for Molecular Dynamics (MD) simulations in order to capture the impact of the R100W mutation on their binding energetic landscapes and to unveil the molecular determinants that trigger their different physiological and pathological outcomes. The present in silico studies highlight that the stability and the binding energetic fingerprints in the 2p75NTR/proNGF complex is not affected by R100W mutation, which on the contrary, deeply affects the energetic landscape, and thus the stability in the p75NTR/NGF complex. Overall, these findings present insights into the structural basis of the molecular mechanisms beyond the clinical manifestations of HSAN V patients.
Marine Plankton during the Polar Night: Environmental Predictors of Spatial Variability
Authors: Dvoretsky Vladimir G. , Venger Marina P. , Vashchenko Anastasya V. , Vodopianova Veronika V. , Pastukhov Ivan A. , Maksimovskaya Tatyana M.
Published: February 2023

We studied the spatial patterns of the planktonic ecosystems at two Arctic sites strongly affected by Atlantic Inflow (FS, the Fram Strait; and BS, the Barents Sea). A high degree of similarity in the bacterial abundance (mean: 3.1 × 105 cells mL−1 in FS vs. 3.5 × 105 cells mL−1 in BS) was found, while other plankton characteristics were different. Bacterial biomass reached a maximum in BS (3.2–7.9 mg C m−3), while viral abundances tended to be higher in FS (2.0–5.7 × 106 particles mL−1). Larger bacterial cells were found in BS, suggesting the presence of different bacterial populations at both locations. The virus-to-bacteria ratio was significantly higher in FS than in BS (13.5 vs. 4.7). Chlorophyll a concentration was extremely low (<0.25 mg m−3). The highest zooplankton abundance was in the surface layer (919 individuals m−3 in FS vs. 602 ind. m−3 in BS). Zooplankton biomass strongly varied (1–39 mg C m−3), with the maximum in BS. High proportions of boreal taxa in the total zooplankton abundance indicate the Atlantification of pelagic ecosystems in the Arctic. Plankton indicators are correlated with temperature, salinity, and sampling depth. Strong intercorrelations were found between major plankton groups, suggesting tight links in the studied plankton ecosystems.
Effective Reversal of Macrophage Polarization by Inhibitory Combinations Predicted by a Boolean Protein–Protein Interaction Model
Authors: Szegvari Gabor , Dora David , Lohinai Zoltan
Published: February 2023

Background: The function and polarization of macrophages has a significant impact on the outcome of many diseases. Targeting tumor-associated macrophages (TAMs) is among the greatest challenges to solve because of the low in vitro reproducibility of the heterogeneous tumor microenvironment (TME). To create a more comprehensive model and to understand the inner workings of the macrophage and its dependence on extracellular signals driving polarization, we propose an in silico approach. Methods: A Boolean control network was built based on systematic manual curation of the scientific literature to model the early response events of macrophages by connecting extracellular signals (input) with gene transcription (output). The network consists of 106 nodes, classified as 9 input, 75 inner and 22 output nodes, that are connected by 217 edges. The direction and polarity of edges were manually verified and only included in the model if the literature plainly supported these parameters. Single or combinatory inhibitions were simulated mimicking therapeutic interventions, and output patterns were analyzed to interpret changes in polarization and cell function. Results: We show that inhibiting a single target is inadequate to modify an established polarization, and that in combination therapy, inhibiting numerous targets with individually small effects is frequently required. Our findings show the importance of JAK1, JAK3 and STAT6, and to a lesser extent STK4, Sp1 and Tyk2, in establishing an M1-like pro-inflammatory polarization, and NFAT5 in creating an anti-inflammatory M2-like phenotype. Conclusions: Here, we demonstrate a protein–protein interaction (PPI) network modeling the intracellular signalization driving macrophage polarization, offering the possibility of therapeutic repolarization and demonstrating evidence for multi-target methods.
Improved Boron Neutron Capture Therapy Using Integrin αvβ3-Targeted Long-Retention-Type Boron Carrier in a F98 Rat Glioma Model
Authors: Tsujino Kohei , Kashiwagi Hideki , Nishimura Kai , Kayama Ryo , Yoshimura Kohei , Fukuo Yusuke , Shiba Hiroyuki , Hiramatsu Ryo , Nonoguchi Naosuke , Furuse Motomasa , Takami Toshihiro , Miyatake Shin-Ichi , Hu Naonori , Takata Takushi , Tanaka Hiroki , Suzuki Minoru , Kawabata Shinji , Nakamura Hiroyuki , Wanibuchi Masahiko
Published: February 2023

Integrin αvβ3 is more highly expressed in high-grade glioma cells than in normal tissues. In this study, a novel boron-10 carrier containing maleimide-functionalized closo-dodecaborate (MID), serum albumin as a drug delivery system, and cyclic arginine-glycine-aspartate (cRGD) that can target integrin αvβ3 was developed. The efficacy of boron neutron capture therapy (BNCT) targeting integrin αvβ3 in glioma cells in the brain of rats using a cRGD-functionalized MID-albumin conjugate (cRGD-MID-AC) was evaluated. F98 glioma cells exposed to boronophenylalanine (BPA), cRGD-MID-AC, and cRGD + MID were used for cellular uptake and neutron-irradiation experiments. An F98 glioma-bearing rat brain tumor model was used for biodistribution and neutron-irradiation experiments after BPA or cRGD-MID-AC administration. BNCT using cRGD-MID-AC had a sufficient cell-killing effect in vitro, similar to that with BNCT using BPA. In biodistribution experiments, cRGD-MID-AC accumulated in the brain tumor, with the highest boron concentration observed 8 h after administration. Significant differences were observed between the untreated group and BNCT using cRGD-MID-AC groups in the in vivo neutron-irradiation experiments through the log-rank test. Long-term survivors were observed only in BNCT using cRGD-MID-AC groups 8 h after intravenous administration. These findings suggest that BNCT with cRGD-MID-AC is highly selective against gliomas through a mechanism that is different from that of BNCT with BPA.
BOX38, a DNA Marker for Selection of Essential Oil Yield of Rosa × rugosa
Authors: Wang Jianwen , Liang Yue , Chu Yadong , Feng Liguo
Published: February 2023

Rosa rugosa L. was a famous aromatic plant whose cultivars (Rosa × rugosa) have been widely used in the perfume industry in Asia. The perfume market looks for rose cultivars bearing higher essential oil, while the oil yields of most R. × rugosa have not been evaluated due to limiting conditions, such as insufficient cultivation areas. Here, we tested the yield and the aroma components of essential oil of 19 R. × rugosa. The results indicated that the yields of nerol, citronellol, and geraniol could represent an alternative index of the total yield of essential oil. Sequence syntenic analysis indicated that the Rosa genus specific cis-element Box38 was highly polymorphic. The Box38 region isolation of Rosa × rugosa by flanked primers proved that Box38 repeat number was significantly positively correlated with the essential oil yield of the corresponding cultivar. In the breeding of Rosa × rugosa, six-Box38-repeat could be a robust threshold for selection of high-essential-oil roses. Together, we found that Box38 was a DNA marker for essential oil yield and that it would be helpful in the early selection and breeding of essential oil roses.
Generalization of the Ratiometric Method to Extend pH Range Measurements of the BCECF Probe
Authors: Tafech Alaa , Beaujean Céline , Usson Yves , Stéphanou Angélique
Published: February 2023

There is a variety of fluorescent probes for pH measurements and which are mainly used for biological systems. In general, they can be classified into two groups. The first group includes fluorescent pH probes which exhibit a single fluorescence emission peak. For these probes, the fluorescence excitation profile is pH-dependent and the shape of the emission spectra remains almost constant. Hence, the ratiometric pH measurement–which makes pH determination independent of the probe concentration-is implemented when the excitation is performed at two excitation wavelengths and the fluorescence emission is measured at one wavelength. The second group exhibits a dual fluorescence emission peak. Here, each protonated or deprotonated form exhibits characteristics emission and/or absorption spectra. Shifts between spectra obtained for protonated and deprotonated species can be exploited in order to perform a ratiometric measurement. In this study we present a methodology that evaluates the precision of the ratiometric measurements based on multiple wavelengths excitation to determine the optimum wavelengths combination for pH determination in biological samples. This methodology using the BCECF probe is applied to measure the pH drift in cell culture medium. It exhibits a high precision and significantly extends the range of validity for pH measurements spanning from very acidic to basic.
Convergence of 3D Bioprinting and Nanotechnology in Tissue Engineering Scaffolds
Authors: Zhang Shike , Chen Xin , Shan Mengyao , Hao Zijuan , Zhang Xiaoyang , Meng Lingxian , Zhai Zhen , Zhang Linlin , Liu Xuying , Wang Xianghong
Published: February 2023

Three-dimensional (3D) bioprinting has emerged as a promising scaffold fabrication strategy for tissue engineering with excellent control over scaffold geometry and microstructure. Nanobiomaterials as bioinks play a key role in manipulating the cellular microenvironment to alter its growth and development. This review first introduces the commonly used nanomaterials in tissue engineering scaffolds, including natural polymers, synthetic polymers, and polymer derivatives, and reveals the improvement of nanomaterials on scaffold performance. Second, the 3D bioprinting technologies of inkjet-based bioprinting, extrusion-based bioprinting, laser-assisted bioprinting, and stereolithography bioprinting are comprehensively itemized, and the advantages and underlying mechanisms are revealed. Then the convergence of 3D bioprinting and nanotechnology applications in tissue engineering scaffolds, such as bone, nerve, blood vessel, tendon, and internal organs, are discussed. Finally, the challenges and perspectives of convergence of 3D bioprinting and nanotechnology are proposed. This review will provide scientific guidance to develop 3D bioprinting tissue engineering scaffolds by nanotechnology.
Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection
Authors: Wang Huiyuan , Wang Xinyu , Lai Keqiang , Yan Juan
Published: February 2023

Food safety has always been a major global challenge to human health and the effective detection of harmful substances in food can reduce the risk to human health. However, the food industry has been plagued by a lack of effective and sensitive safety monitoring methods due to the tension between the cost and effectiveness of monitoring. DNA-based hydrogels combine the advantages of biocompatibility, programmability, the molecular recognition of DNA molecules, and the hydrophilicity of hydrogels, making them a hotspot in the research field of new nanomaterials. The stimulus response property greatly broadens the function and application range of DNA hydrogel. In recent years, DNA hydrogels based on stimulus-responsive mechanisms have been widely applied in the field of biosensing for the detection of a variety of target substances, including various food contaminants. In this review, we describe the recent advances in the preparation of stimuli-responsive DNA hydrogels, highlighting the progress of its application in food safety detection. Finally, we also discuss the challenges and future application of stimulus-responsive DNA hydrogels.
Application of Proteomics Analysis and Animal Models in Optic Nerve Injury Diseases
Authors: Meng Zhaoyang , You Ran , Mahmood Arif , Yan Fancheng , Wang Yanling
Published: February 2023

Optic nerve damage is a common cause of blindness. Optic nerve injury is often accompanied by fundus vascular disease, retinal ganglion cell apoptosis, and changes in retinal thickness. These changes can cause alterations in protein expression within neurons in the retina. Proteomics analysis offers conclusive evidence to decode a biological system. Furthermore, animal models of optic nerve injury made it possible to gain insight into pathological mechanisms, therapeutic targets, and effective treatment of such injuries. Proteomics takes the proteome as the research object and studies protein changes in cells and tissues. At present, a variety of proteomic analysis methods have been widely used in the research of optic nerve injury diseases. This review summarizes the application of proteomic research in optic nerve injury diseases and animal models of optic nerve injury. Additionally, differentially expressed proteins are summarized and analyzed. Various optic nerve injuries, including those associated with different etiologies, are discussed along with their potential therapeutic targets and future directions.
Color-Coding Method Reveals Enhancement of Stereotypic Locomotion by Phenazepam in Rat Open Field Test
Authors: Makarov Mark , Sysoev Yuri I. , Agafonova Oksana , Prikhodko Veronika A. , Korkotian Eduard , Okovityi Sergey V.
Published: February 2023

One of the most important tasks in neuroscience is the search for theoretical foundations for the development of methods for diagnosing and treating neurological pathology, and for assessing the effect of pharmacological drugs on the nervous system. Specific behavioral changes associated with exposure to systemic influences have been invisible to the human eye for a long time. A similar pattern of changes is characteristic of phenazepam, a drug with a wide range of effects on the brain. In this study, we used a color-coding method, which consists of combining three time positions in one image, the present (0 s), the near future (0.33 s) and the far future (1.6 s). This method made it possible to identify movement patterns, such as the initialization of ahead movements, side turns and 180° turns (back), and also to determine the degree of predictability of future movements. The obtained data revealed a decrease in the number of turns to the sides while maintaining ahead movement, as well as an increase in the predictability of movements in rats under the influence of phenazepam. Thus, sedative doses of phenazepam do not exhibit general depression of brain functions, but the inhibition of specific centers, including the medial prefrontal cortex and postsubiculum, which are involved in stereotypic locomotive behavior.
Technical Pearls to Effectively Use 5-ALA in Fluorescence-Guided Tumor Resection—5 Lessons from the Operating Room
Authors: Della Pepa Giuseppe Maria , Menna Grazia , Olivi Alessandro
Published: February 2023

Background: Since its introduction in 2007 in Europe and in 2017 in the United States, 5-ALA has demonstrated an undisputed advantage in providing real-time tumor visualization. The aim of the present paper is to summarize our institutional experience over a decade of routine 5-ALA-guided procedures in order to provide five surgical tricks to ease surgical workflow. Methods: Data were collected from 822 patients diagnosed with histopathologically confirmed high-grade gliomas (HGG)—according to the WHO 2021 criteria—who underwent surgery at the Fondazione Policlinico Universitario Agostino Gemelli between January 2012 and January 2022. Results: From our large institutional experience, the learned technical pearls were grouped in five distinct domains: 1. Analysis of visualization, overall workflow, and technical recommendations to improve intraoperative set-up; 2. Techniques to reduce the risk of inadvertent residuals and failure to evocate fluorescence; 3. Analysis of specific surgical conditions favoring remnants; 4. Assessment of different degrees of fluorescence and their surgical meaning; 5. Analysis of false positive cases. Conclusions: With all the limitations of a qualitative and retrospective analysis, this paper was specifically conceived as a vademecum for educational purposes to promote and maximize 5-ALA employment.
Influencing Factors in Visual Preference Assessment of Redeveloped Urban Villages in China: A Case Study of Guangdong Province
Authors: Shen Jiamin , Han Chenping , Li Guanjun , Wang Xinyu
Published: February 2023

The urban village represents a particular problem in urban design and renewal in China. Many cities in China have started the redevelopment of urban villages. Based on the investigation of four urban village redevelopment projects in Guangdong Province, China from 2010 to 2020, building façades, plant landscape, roads, and municipal public facility variety were taken as physical factors in this study. Urban village residents with different demographic characteristics, such as gender, age, income, family size, and urban village-native status, were selected as respondents, and the influence of the considered village physical factors on the visual preference assessments performed by the respondents was analyzed by means of photo stimulation. The results show that all four village factors exerted a certain influence on the respondents’ visual preference assessment. Redeveloped urban villages presenting repaired and decorated building façades, various species of plants, resurfaced roads, and medium municipal public facility variety were favored by the respondents. Urban village residents with different demographic characteristics also provided different visual preference assessments of different physical factors of the redeveloped urban villages.
Comparative Study on the Effects of Five Nano-Metallic Oxide Particles on the Long-Term Mechanical Property and Durability of Cement Mortar
Authors: Dong Shuangkuai , Yu Songhan , Chen Liangliang , Zhuo Qi , Wu Fufei , Xie Lilan , Liu Liuyan
Published: February 2023

Nano-metallic oxide particles have been found to be potentially effective microstructural reinforcements for cement mortar and have become a research hotspot in recent years for nano-modification technology of building materials. However, different conclusions have been obtained due to various researchers used different research methods, which have resulted in a deficiency for the performance comparison between different nano-metallic oxide particles. In the present study, the effects of five kinds of nano-metallic oxide particles, namely nano-MgO, nano-Al2O3, nano-ZrO2, nano-CuO, and nano-ZnO, on the performance of cement mortar at 28 days and 730 days in terms of mechanical, durability, microstructure, and pore size distribution properties by performing different experiments were investigated. Test results show that the dosage of nano-MgO, nano-Al2O3, nano-ZrO2, nano-CuO, and nano-ZnO is 2%, 1%, 1%, 1%, and 2%, respectively, where they can significantly prove the compressive and flexural strengths, decrease the porosity, drying shrinkage, and permeability, and refine the pore size distribution of cement mortar. It can be seen through SEM analysis that nano-metallic oxide particles can promote cement hydration, and also refine the size and distribution of Ca(OH)2 crystal, but the specific principles are different. The analysis concluded that the five kinds of nano-metallic oxide particles can play a filling role in cementitious materials to improve the denseness and surface activity role to promote the hydration of cement particles, thus improving the mechanical properties, durability, and pore size distribution of cementitious materials, with the order of their modification effect on cement-based materials being nano-ZrO2 > nano-MgO > nano-Al2O3 > nano-ZnO > nano-CuO.
Selection of Response Reduction Factor Considering Resilience Aspect
Authors: Prasanth S. , Ghosh Goutam , Gupta Praveen Kumar , Kumar Virendra , Paramasivam Prabhu , Dhanasekaran Seshathiri
Published: February 2023

The selection of an adequate response reduction factor (R) in the seismic design of a reinforced concrete building is critical to the building’s seismic response. To construct a robust structure, the R factor should be chosen based on the building’s resilience performance. Since no background was provided for the selection of R factors, the study focuses on the right selection of R factors in relation to the building’s functionality, performance level, and resilience. In this study, a high-rise building with multiple R factors (R = 3, 4, 5, and 6) is developed. Five potential recovery paths (RP-1 to RP-5) that matched the realistic scenario were used to estimate the building’s functionality. The building was subjected to uni and bi-directional loadings, and two design levels, Design Basic Earthquake (DBE) and Maximum Considered Earthquake were used to monitor the building’s response. According to the findings, a decrease in the lateral design force with the highest R results in a high ductility requirement and a substantial loss of resilience. The maximum R factor can be recommended under uni-directional loading up to 6, in which the building’s resilience is almost 50%, whereas under bi-directional loading and taking the recommended R factor decreased from 6 to 4.
Forecasting Offline Retail Sales in the COVID-19 Pandemic Period: A Case Study of a Complex Shopping Mall in South Korea
Authors: Kim Hee-Jeong , Kim Ju-Hyung , Im Jin-bin
Published: February 2023

This study examines the case of a shopping mall in Seoul, South Korea, based on its offline retail sales data during the period of the enforcement of the COVID-19 pandemic social distancing policy. South Korea implemented strict social distancing, especially in retail categories where people eat out, due to the danger of spreading infectious disease. A total of 55 retail shops’ sales data were analyzed and classified into five categories: fashion, food and beverage (f&b), entertainment, cosmetics and sport. Autoregressive integrated moving average (ARIMA) and exponential smoothing (ETS) models were employed, and the autocorrelation (ACF) and partial autocorrelation (PACF) of each retail category’s sales data were analyzed. The mean absolute percentage error (MAPE) was used to determine the most suitable forecasting model for each retail category. In this way, the f&b and entertainment retail categories, in which people eat out, were found to have been significantly impacted, with their 2022 sales forecasted to be less than 80% of their 2018 and 2019 sales. The fashion retail category was also significantly impacted, slowly recovering sales in 2022. The cosmetics and sport retail categories were little impacted by the COVID-19 outbreak, with their retail sales having already recovered by 2022.
Role of Surgery in Patients with Stage IE Primary Thyroid MALT Lymphoma Staged by a Modified Classification System: The Tokyo Classification
Authors: Saito Yoshiyuki , Watanabe Natsuko , Suzuki Nami , Saito Naoko , Narimatsu Hiroto , Takami Hiroshi , Kameyama Kaori , Yoshioka Kana , Masaki Chie , Akaishi Junko , Hames Kiyomi Yamada , Matsumoto Masako , Fukushita Miho , Yoshihara Ai , Okamura Ritsuko , Tomoda Chisato , Suzuki Akifumi , Matsuzu Kenichi , Kitagawa Wataru , Nagahama Mitsuji , Noh Jaeduk Yoshimura , Sugino Kiminori , Ito Koichi
Published: February 2023

Purposes: To establish the appropriate staging system and assess the role of curative thyroidectomy alone (Surgery) vs. involved-site radiation therapy after open biopsy (OB-ISRT) in stage IE mucosa-associated lymphoid tissue (MALT) lymphoma. Methods: We examined the Tokyo Classification as a modified classification. This retrospective cohort study included 256 patients with thyroid MALT lymphoma; 137 underwent standard therapy (i.e., OB-ISRT) and were enrolled for the Tokyo classification. Sixty stage IE patients with the same diagnosis were examined to compare Surgery with OB-ISRT. Results: Overall survival (p = 0.0092) and relapse-free survival (0.00113) were significantly better in stage IE vs. stage IIE under the Tokyo classification. No OB-ISRT and Surgery patients died, but three OB-ISRT patients relapsed. The incidence of permanent complications was 28% in OB-ISRT (mainly dry mouth) and 0% in Surgery (p = 0.027). The number of painkiller prescription days was significantly greater in OB-ISRT (p < 0.001). During follow-up, the rate of the new appearance/change of the low-density area in the thyroid gland was significantly higher in OB-ISRT (p = 0.031). Conclusions: The Tokyo classification allows an appropriate discrimination between stages IE and IIE MALT lymphoma. Surgery can provide a good prognosis in stage IE cases; it also avoids complications, shortens painful periods during treatment, and simplifies ultrasound follow-up.
A Systematic Review of High-Dose Methotrexate for Adults with Primary Central Nervous System Lymphoma
Authors: Villanueva Gabriela , Guscott Martin , Schaiquevich Paula , Sampor Claudia , Combs Ryan , Tentoni Nicolás , Hwang Miriam , Lowe Jennifer , Howard Scott
Published: February 2023

Primary central nervous system lymphoma (PCNSL) is a highly aggressive non-Hodgkin lymphoma that is confined within the CNS. Due to its ability to cross the blood–brain barrier, high-dose methotrexate (HDMTX) is the backbone for induction chemotherapy. This systematic review was conducted to observe outcomes among different HDMTX doses (low, <3 g/m2; intermediate, 3–4.9 g/m2; high, ≥5 g/m2) and regimens used in the treatment of PCNSL. A PubMed search resulted in 26 articles reporting clinical trials using HDMTX for PCNSL, from which 35 treatment cohorts were identified for analysis. The median dose of HDMTX used for induction was 3.5 g/m2 (interquartile range IQR, 3–3.5); the intermediate dose was most frequently used in the studies examined (24 cohorts, 69%). Five cohorts used HDMTX monotherapy, 19 cohorts used HDMTX + polychemotherapy, and 11 cohorts used HDMTX + rituximab ± polychemotherapy. Pooled overall response rate (ORR) estimates for low, intermediate, and high dose HDMTX cohorts were 71%, 76%, and 76%, respectively. Pooled 2-year progression-free survival (PFS) estimates for low, intermediate, and high HDMTX dose cohorts were 50%, 51%, and 55%, respectively. Regimens that included rituximab showed a tendency to have higher ORR and 2-year PFS than those that did not include rituximab. These findings indicate that current protocols utilizing 3–4 g/m2 of HDMTX in combination with rituximab provide therapeutic efficacy in PCNSL.
Influence of Clinical and Surgical Factors on Uterine Carcinosarcoma Survival
Authors: Gracia Myriam , Yildirim Yusuf , Macuks Ronalds , Mancari Rosanna , Achimas-Cadariu Patriciu , Polterauer Stephan , Iacoponi Sara , Zapardiel Ignacio
Published: February 2023

Background: The aim of this study was to assess the impact of prognostic factors on the survival of patients diagnosed with uterine carcinosarcoma. Methods: A sub-analysis of the SARCUT study, a multicentric retrospective European study, was carried out. We selected 283 cases of diagnosed uterine carcinosarcoma for the present study. Prognosis factors influencing survival were analyzed. Results: Significant prognostic factors for overall survival were: incomplete cytoreduction (HR = 4.02; 95%CI = 2.68–6.18), FIGO stages III and IV (HR = 3.21; 95%CI = 1.83–5.61), tumor persistence after any treatment (HR = 2.90; 95%CI = 1.97–4.27), presence of extrauterine disease (HR = 2.62; 95%CI = 1.75–3.92), a positive resection margin (HR = 1.56; 95%CI = 1.05–2.34), age (HR = 1.02; 95%CI = 1.00–1.05), and tumor size (HR = 1.01; 95%CI = 1.00–1.01). Significant prognostic factors for disease-free survival were: incomplete cytoreduction (HR = 3.00; 95%CI = 1.67–5.37), tumor persistence after any treatment (HR = 2.64; 95%CI = 1.81–3.86), FIGO stages III and IV (HR = 2.33; 95%CI = 1.59–3.41), presence of extrauterine disease (HR = 2.13; 95%CI = 1.44–3.17), administration of adjuvant chemotherapy (HR = 1.84; 95%CI = 1.27–2.67), a positive resection margin (HR = 1.65; 95%CI = 1.11–2.44), presence of LVSI (HR = 1.61; 95%CI = 1.02–2.55), and tumor size (HR = 1.00; 95%CI = 1.00–1.01). Conclusions: Incomplete cytoreduction, presence of tumor residual after treatment, advanced FIGO stage, extrauterine disease, and tumor size are significant prognostic factors decreasing disease-free survival and overall survival of patients with uterine carcinosarcoma.
Association between the Processed Dietary Pattern and Tumor Staging in Patients Newly Diagnosed with Head and Neck Squamous Cell Carcinoma
Authors: Lima Ana Carolina da Silva , Ferreira Tathiany Jéssica , Campos Adriana Divina de Souza , Matida Larissa Morinaga , Castro Maria Beatriz Trindade , Freitas-Vilela Ana Amélia , Horst Maria Aderuza
Published: February 2023

Purpose: This study aimed to assess the association between dietary patterns and tumor staging and the degree of cell differentiation in patients with head and neck squamous cell carcinoma (HNSCC). Methods: This cross-sectional study included 136 individuals newly diagnosed with different stages of HNSCC, aged 20- to 80 years-old. Dietary patterns were determined by principal component analysis (PCA), using data collected from a food frequency questionnaire (FFQ). Anthropometric, lifestyle, and clinicopathological data were collected from patients’ medical records. Disease staging was categorized as initial stage (stages I and II), intermediary (stage III), and advanced (stage IV). Cell differentiation was categorized as poor, moderate, or well-differentiated. The association of dietary patterns with tumor staging and cell differentiation was evaluated using multinomial logistic regression models and adjusted for potential confounders. Results: Three dietary patterns, “healthy,” “processed,” and “mixed,” were identified. The “processed” dietary pattern was associated with intermediary (odds ratio (OR) 2.47; 95% confidence interval (CI) 1.43–4.26; p = 0.001) and advanced (OR 1.78; 95% CI 1.12–2.84; p = 0.015) staging. No association was found between dietary patterns and cell differentiation. Conclusion: A high adherence to dietary patterns based on processed foods is associated with advanced tumor staging in patients newly diagnosed with HNSCC.
Involvement of Epithelial–Mesenchymal Transition Genes in Small Cell Lung Cancer Phenotypic Plasticity
Authors: Groves Sarah M. , Panchy Nicholas , Tyson Darren R. , Harris Leonard A. , Quaranta Vito , Hong Tian
Published: February 2023

Small cell lung cancer (SCLC) is an aggressive cancer recalcitrant to treatment, arising predominantly from epithelial pulmonary neuroendocrine (NE) cells. Intratumor heterogeneity plays critical roles in SCLC disease progression, metastasis, and treatment resistance. At least five transcriptional SCLC NE and non-NE cell subtypes were recently defined by gene expression signatures. Transition from NE to non-NE cell states and cooperation between subtypes within a tumor likely contribute to SCLC progression by mechanisms of adaptation to perturbations. Therefore, gene regulatory programs distinguishing SCLC subtypes or promoting transitions are of great interest. Here, we systematically analyze the relationship between SCLC NE/non-NE transition and epithelial to mesenchymal transition (EMT)—a well-studied cellular process contributing to cancer invasiveness and resistance—using multiple transcriptome datasets from SCLC mouse tumor models, human cancer cell lines, and tumor samples. The NE SCLC-A2 subtype maps to the epithelial state. In contrast, SCLC-A and SCLC-N (NE) map to a partial mesenchymal state (M1) that is distinct from the non-NE, partial mesenchymal state (M2). The correspondence between SCLC subtypes and the EMT program paves the way for further work to understand gene regulatory mechanisms of SCLC tumor plasticity with applicability to other cancer types.
Tumor Microenvironment and Immune Response in Lip Cancer
Authors: Gkegka Anastasia G. , Koukourakis Michael I. , Lambropoulou Maria , Giatromanolaki Alexandra
Published: February 2023

Tumor-infiltrating lymphocytes (TILs) play a significant role in cancer progression and prognosis of patients. The tumor microenvironment (TME) may affect the anti-tumor immune response. We examined the TIL and tertiary lymphoid structure (TLS) density in the invading front and inner tumor stroma, and the lymphocyte subpopulation (CD8, CD4, FOXP3) density in 60 squamous cell carcinomas of the lip. Analysis was performed in parallel with markers of hypoxia (hypoxia-inducible factor (HIF1α), lactate dehydrogenase (LDHA)) and angiogenesis. Low TIL density in the invading tumor front was related with larger tumor size (p = 0.05), deep invasion (p = 0.01), high smooth-muscle actin (SMA) expression (p = 0.01), and high HIF1α and LDH5 expression (p = 0.04). FOXP3+ TILs infiltration and FOXP3+/CD8+ ratios were higher in inner tumor areas, linked with LDH5 expression, and higher MIB1 proliferation index (p = 0.03) and SMA expression (p = 0.001). Dense CD4+ lymphocytic infiltration in the invading front is related to high tumor-budding (TB) (p = 0.04) and angiogenesis (p = 0.04 and p = 0.006, respectively). Low CD8+ TIL density, high CD20+ B-cell density, high FOXP3+/CD8+ ratio and high CD68+ macrophage presence characterized tumors with local invasion (p = 0.02, 0.01, 0.02 and 0.006, respectively). High angiogenic activity was linked with high CD4+, FOXP3+, and low CD8+ TIL density (p = 0.05, 0.01 and 0.01, respectively), as well as high CD68+ macrophage presence (p = 0.003). LDH5 expression was linked with high CD4+ and FOXP3+ TIL density (p = 0.05 and 0.01, respectively). Further research is needed to explore the prognostic and therapeutic value of TME/TIL interactions.