bluuebunny/crossref_metadata_2025 huggingface dataset:
3D visualization technology for rubber tree forests based on a terrestrial photogrammetry system
Authors: Lei Shuhan , Liu Li , Xie Yu , Fang Ying , Wang Chuangxia , Luo Ninghao , Li Ruitao , Yu Donghai , Qiu Zixuan
Published: August 2023

Introduction

Rubber trees are an important cash crop in Hainan Province; thus, monitoring sample plots of these trees provides important data for determining growth conditions. However, existing monitoring technology and rubber forest sample plot analysis methods are relatively simple and present widespread issues, such as limited monitoring equipment, transportation difficulties, and relatively poor three-dimensional visualization effects in complex environments. These limitations have complicated the development of rubber forest sample plot monitoring.

Method

This study developed a terrestrial photogrammetry system combined with 3D point-cloud reconstruction technology based on the structure from motion with multi-view stereo method and sample plot survey data. Deviation analyses and accuracy evaluations of sample plot information were performed in the study area for trees to explore the practical significance of this method for monitoring rubber forest sample plots. Furthermore, the relationship between the height of the first branch, diameter at breast height (DBH), and rubber tree volume was explored, and a rubber tree standard volume model was established.

Results

The Bias, relative Bias, RMSE, and RRMSE of the height of the first branch measured by this method were −0.018 m, −0.371%, 0.562 m, and 11.573%, respectively. The Bias, relative Bias, RMSE, and RRMSE of DBH were −0.484 cm, −1.943%, −2.454 cm, and 9.859%, respectively, which proved that the method had high monitoring accuracy and met the monitoring requirements of rubber forest sample plots. The fitting results of rubber tree standard volume model had an R2 value of 0.541, and the estimated values of each parameter were 1.745, 0.115, and 0.714. The standard volume model accurately estimated the volume of rubber trees and forests using the first branch height and DBH.

Discussion

This study proposed an innovative planning scheme for a terrestrial photogrammetry system for 3D visual monitoring of rubber tree forests, thus providing a novel solution to issues observed in current sample plot monitoring practices. In the future, the application of terrestrial photogrammetry systems to monitor other types of forests will be explored.
Progress in construction of mouse models to investigate the pathogenesis and immune therapy of human hematological malignancy
Authors: Lang Yue , Lyu Yanan , Tan Yehui , Hu Zheng
Published: August 2023

Hematological malignancy is a disease arisen by complicate reasons that seriously endangers human health. The research on its pathogenesis and therapies depends on the usage of animal models. Conventional animal model cannot faithfully mirror some characteristics of human features due to the evolutionary divergence, whereas the mouse models hosting human hematological malignancy are more and more applied in basic as well as translational investigations in recent years. According to the construction methods, they can be divided into different types (e.g. cell-derived xenograft (CDX) and patient-derived xenograft model (PDX) model) that have diverse characteristics and application values. In addition, a variety of strategies have been developed to improve human hematological malignant cell engraftment and differentiation in vivo. Moreover, the humanized mouse model with both functional human immune system and autologous human hematological malignancy provides a unique tool for the evaluation of the efficacy of novel immunotherapeutic drugs/approaches. Herein, we first review the evolution of the mouse model of human hematological malignancy; Then, we analyze the characteristics of different types of models and summarize the ways to improve the models; Finally, the way and value of humanized mouse model of human immune system in the immunotherapy of human hematological malignancy are discussed.
Therapeutic potential of Litsea cubeba essential oil in modulating inflammation and the gut microbiome
Authors: Xia Liqiong , Li Ran , Tao Ting , Zhong Ruimin , Du Haifang , Liao Ziling , Sun Zhanghua , Xu Changqiong
Published: August 2023

Inflammation, a sophisticated and delicately balanced physiological mechanism, is paramount to the host’s immunological defense against pathogens. However, unfettered and excessive inflammation can be instrumental in engendering a plethora of chronic ailments and detrimental health repercussions, notably within the gastrointestinal tract. Lipopolysaccharides (LPS) from bacteria are potent endotoxins capable of instigating intestinal inflammation through the disruption of the intestinal epithelial barrier and the stimulation of a pro-inflammatory immune response. In this study, we sought to investigate the influence of Litsea cubeba essential oil (LCEO) on LPS-induced intestinal inflammation and associated changes in the gut microbiota. We investigated the therapeutic potential of LCEO for gut health, with particular emphasis on its gut protective properties, anti-inflammatory properties and modulation of the gut microbiome. LCEO exhibited protective effects on colonic tissue by protecting crypts and maintaining epithelial integrity, and anti-inflammatory properties by reducing TNF-α, IL-6, and IL-1β levels in the liver and intestine. Citral, a major component of LCEO, showed robust binding to IL-1β, IL-6, and TNF-α, exerting anti-inflammatory effects through hydrogen bonding interactions. Using community barplot and LEfSe analyses, we detected significant variation in microbial composition, identified discrete biomarkers, and highlighted the influence of essential oils on gut microbial communities. Our research suggests that LCEO may be a promising natural compound for ameliorating diarrhea and intestinal inflammation, with potential implications for modulating the gut microbiome. These observations provide invaluable insight into the potential therapeutic role of LCEO as a natural anti-inflammatory agent for treating intestinal inflammatory disorders, particularly in the setting of a dysregulated immune response and altered gut microbiota. Furthermore, our findings highlight the need to understand the complex interplay between the host, the gut microbiome and natural products in the context of inflammatory diseases.
Profiling neurotransmitter-evoked glial responses by RNA-sequencing analysis
Authors: Wang Mengxiao , Ho Margaret S.
Published: August 2023

Fundamental properties of neurons and glia are distinctively different. Neurons are excitable cells that transmit information, whereas glia have long been considered as passive bystanders. Recently, the concept of tripartite synapse is proposed that glia are structurally and functionally incorporated into the synapse, the basic unit of information processing in the brains. It has then become intriguing how glia actively communicate with the presynaptic and postsynaptic compartments to influence the signal transmission. Here we present a thorough analysis at the transcriptional level on how glia respond to different types of neurotransmitters. Adult fly glia were purified from brains incubated with different types of neurotransmitters ex vivo. Subsequent RNA-sequencing analyses reveal distinct and overlapping patterns for these transcriptomes. Whereas Acetylcholine (ACh) and Glutamate (Glu) more vigorously activate glial gene expression, GABA retains its inhibitory effect. All neurotransmitters fail to trigger a significant change in the expression of their synthesis enzymes, yet Glu triggers increased expression of neurotransmitter receptors including its own and nAChRs. Expressions of transporters for GABA and Glutamate are under diverse controls from DA, GABA, and Glu, suggesting that the evoked intracellular pathways by these neurotransmitters are interconnected. Furthermore, changes in the expression of genes involved in calcium signaling also functionally predict the change in the glial activity. Finally, neurotransmitters also trigger a general metabolic suppression in glia except the DA, which upregulates a number of genes involved in transporting nutrients and amino acids. Our findings fundamentally dissect the transcriptional change in glia facing neuronal challenges; these results provide insights on how glia and neurons crosstalk in a synaptic context and underlie the mechanism of brain function and behavior.
Application of microcatheter shaping based on computational fluid dynamics simulation of cerebral blood flow in the intervention of posterior communicating aneurysm of the internal carotid artery
Authors: Xu Gangqin , Ba Yueyang , Zhang Kun , Cai Dongyang , Yang Bowen , Zhao Tongyuan , Xue Jiangyu
Published: August 2023

Introduction

The present study aimed to investigate the application of the aneurysm embolization microcatheter plasticity method based on computational fluid dynamics (CFD) to simulate cerebral blood flow in the interventional treatment of posterior communicating aneurysms in the internal carotid artery and to evaluate its practicality and safety.

Methods

A total of 20 patients with posterior internal carotid artery communicating aneurysms who used CFD to simulate cerebral flow lines from January 2020 to December 2022 in our hospital were analyzed. Microcatheter shaping and interventional embolization were performed according to the main cerebral flow lines, and the success rate, stability, and effect of the microcatheter being in place were analyzed.

Results

Among the 20 patients, the microcatheters were all smoothly placed and the catheters were stable during the in vitro model test. In addition, the microcatheters were all smoothly placed during the operation, with a success rate of 100%. The catheter tips were stable and well-supported intraoperatively, and no catheter prolapse was registered. The aneurysm was completely embolized in 19 cases immediately after surgery, and a small amount of the aneurysm neck remained in one case. There were no intraoperative complications related to the embolization catheter operation.

Conclusion

Microcatheter shaping based on CFD simulation of cerebral blood flow, with precise catheter shaping, leads to a high success rate in catheter placing, stability, and good support, and greatly reduces the difficulty of catheter shaping. This catheter-shaping method is worthy of further study and exploration.
Brief report: risk stratification following curative therapy for stage I NSCLC
Authors: Butts Emily , Gococo-Benore Denise , Pai Tanmayi , Moustafa Muhamad Alhaj , Heng Fei , Chen Ruqin , Zhao Yujie , Manochakian Rami , Lou Yanyan
Published: August 2023

Introduction

Surveillance with computed tomography (CT) imaging following curative treatment of stage I non-small cell lung cancer (NSCLC) is important to identify recurrence or second primary lung cancers (SPLC). The pattern and risks of recurrence following curative therapy and optimal duration of surveillance scans remain unknown. The objective of our study is to assess the pattern of recurrence and development of SPLC to risk stratify patients with stage I NSCLC following curative therapy.

Methods

We identified 261 patients who received curative therapy for stage I NSCLC at Mayo Clinic Florida. Data was collected on clinical and demographic features including gender, smoking history, stage, treatment, histologic subtype, and tumor grade. Kaplan-Meier method was used to evaluate the disease free survival (DFS). Cox proportional hazard model was used to identify risk factors for recurrence.

Results

Negative tobacco history and stage IA tumors were associated with significantly prolonged DFS after adjusting for co-variates (p=0.001 and p=0.005). Univariate Cox proportional hazards model identified tobacco history and stage 1B as risk factors for recurrence with unadjusted hazard ratio (HR) of 2.8 and 2.0, respectively. After adjusting for covariates, only stage IB was statistically significant predictor of recurrence with a hazard ratio of 2.1 (Confidence Interval (CI) 95% 1.2-3.6; p=0.007).

Conclusions

An individualized approach that considers risk factors of stage and smoking history may be useful in determining whether to continue annual CT surveillance after five years post curative therapy for stage I NSCLC.
Reimbursement of pharmacogenetic tests at a tertiary academic medical center in the United States
Authors: Lemke Lauren K. , Alam Benish , Williams Roy , Starostik Petr , Cavallari Larisa H. , Cicali Emily J. , Wiisanen Kristin
Published: August 2023

Introduction: Pharmacogenetics (PGx) has the potential to improve health outcomes but cost of testing is a barrier for equitable access. Reimbursement by insurance providers may lessen the financial burden for patients, but the extent to which PGx claims are covered in clinical practice has not been well-characterized in the literature.

Methods: A retrospective analysis of outpatient claims submitted to payers for PGx tests from 1/1/2019 through 12/31/2021 was performed. A reimbursement rate was calculated and compared across specific test types (e.g., single genes, panel), payers, indication, and the year the claim was submitted.

Results: A total of 1,039 outpatient claims for PGx testing were analyzed. The overall reimbursement rate was 46% and ranged from 36%–48% across payers. PGx panels were reimbursed at a significantly higher rate than single gene tests (74% vs. 43%, p < 0.001).

Discussion: Reimbursement of claims for PGx testing is variable based on the test type, indication, year the claim was submitted, number of diagnosis codes submitted, and number of unique diagnosis codes submitted. Due to the highly variable nature of reimbursement, cost and affordability should be discussed with each patient.
Melatonin as a regulator of apoptosis in leukaemia: molecular mechanism and therapeutic perspectives
Authors: Mafi Alireza , Rismanchi Hamidreza , Gholinezhad Yasaman , Mohammadi Mohaddese Malek , Mousavi Vahide , Hosseini Seyed Ali , Milasi Yaser Eshaghi , Reiter Russel J. , Ghezelbash Behrooz , Rezaee Malihe , Sheida Amirhossein , Zarepour Fatemeh , Asemi Zatollah , Mansournia Mohammad Ali , Mirzaei Hamed
Published: August 2023

Leukaemia is a dangerous malignancy that causes thousands of deaths every year throughout the world. The rate of morbidity and mortality is significant despite many advancements in therapy strategies for affected individuals. Most antitumour medications used now in clinical oncology use apoptotic signalling pathways to induce cancer cell death. Accumulated data have shown a direct correlation between inducing apoptosis in cancer cells with higher tumour regression and survival. Until now, the efficacy of melatonin as a powerful antitumour agent has been firmly established. A change in melatonin concentrations has been reported in multiple tumours such as endometrial, hematopoietic, and breast cancers. Findings show that melatonin’s anticancer properties, such as its prooxidation function and ability to promote apoptosis, indicate the possibility of utilizing this natural substance as a promising agent in innovative cancer therapy approaches. Melatonin stimulates cell apoptosis via the regulation of many apoptosis facilitators, including mitochondria, cytochrome c, Bcl-2, production of reactive oxygen species, and apoptosis receptors. This paper aimed to further assess the anticancer effects of melatonin through the apoptotic pathway, considering the role that cellular apoptosis plays in the pathogenesis of cancer. The effect of melatonin may mean that it is appropriate for use as an adjuvant, along with other therapeutic approaches such as radiotherapy and chemotherapy.
Disitamab vedotin in combination with immune checkpoint inhibitors for locally and locally advanced bladder urothelial carcinoma: a two-center’s real-world study
Authors: Wei Yongbao , Zhang Ruochen , Yu Chenbo , Hong Zhiwei , Lin Le , Li Tao , Chen Jianhui
Published: August 2023

Objective: Our study aims to assess the effectiveness and safety profile of Disitamab Vedotin (DV, RC48-ADC), an innovative humanized anti-HER2 antibody conjugated with tubulin-disrupting antimitotic drug monomethyl auristatin E (MMAE) via a cleavable peptide linker. This treatment combined immune checkpoint inhibitors as part of the bladder sparing approach for selected patients suffering from locally and locally advanced bladder urothelial carcinoma.

Patients and methods: We conducted a two-center, real-world study involving locally advanced urothelial carcinoma (UC) patients. Patients were classified based on HER2 expression (IHC 3+/2+/1+) or lack of HER2 expression (IHC 0). The primary endpoint was the objective response rate (ORR), assessed by the investigator following the criteria of RECIST V1.1. Secondary endpoints encompassed the pathological complete response rate (pCR), pathological partial response rate (pPR), and pathological stable disease (pSD), along with recurrence-free survival (RFS), the pathological downstaging rate, and the safety profile of the treatment.

Results: In this study, nine patients were enrolled, with a median follow-up duration of 12.0 months. The overall confirmed ORR was 88.9%, Five patients achieved a complete response (CR), and three patients achieved a partial response (PR). The radiological complete response (rCR) aligned perfectly with pCR. The median radiological progression-free survival (rPFS) spanned 12.0 months (range from 8.0 to 17.0 months). One patient diagnosed with disease progression (PD) underwent a radical cystectomy. The pathological stage evolved from T2N0M0 to T3aN2M0, followed by adjuvant chemotherapy with a gemcitabine-cisplatin (GC) combination radiotherapy. At the 9-month follow-up, neither recurrence nor metastasis was observed. The rate and intensity of complications were manageable among these patients, with no evidence of grade 4 and 5 adverse events.

Conclusion: The combination of DV and PD-1 demonstrated considerable activity in the objective response rate (ORR) in patients with HER2 IHC 0/1+/2+/3+ muscle-invasive bladder cancer (MIBC), along with the longest reported median radiological progression-free survival (rPFS) to date. With an extended duration of treatment, the safety profile of DV plus PD-1 was also confirmed to be manageable.
Multiomics studies with co-transformation reveal microRNAs via miRNA-TF-mRNA network participating in wood formation in Hevea brasiliensis
Authors: Chen Jinhui , Liu Mingming , Meng Xiangxu , Zhang Yuanyuan , Wang Yue , Jiao Nanbo , Chen Jianmiao
Published: August 2023

Introduction

MicroRNAs (miRNAs) are small endogenous non-coding RNAs that play an important role in wood formation in plants. However, the significance of the link between miRNAs and their target transcripts in wood formation remains unclear in rubber tree (Hevea brasiliensis).

Methods

In this study, we induced the formation of reaction wood by artificially bending rubber trees for 300 days and performed small RNA sequencing and transcriptome deep sequencing (RNA-seq) to describe the complement of miRNAs and their targets contributing to this process.

Results and discussion

We identified 5, 11, and 2 differentially abundant miRNAs in normal wood (NW) compared to tension wood (TW), in NW relative to opposite wood (OW), and between TW and OW, respectively. We also identified 12 novel miRNAs and 39 potential miRNA-mRNA pairs with different accumulation patterns in NW, TW, and OW. We noticed that many miRNAs targeted transcription factor genes, which were enriched in KEGG pathways associated with phenylpropanoid biosynthesis, phenylalanine metabolism, and pyruvate metabolism. Thus, miRNA-TF-mRNA network involved in wood formation via tension wood model were constructed. We validated the differential accumulation of miRNAs and their targets by RT-qPCR analysis and overexpressed miRNA in Nicotiana benthamiana with its potential target gene. These results will provide a reference for a deep exploration of growth and development in rubber tree.
Stress, mental health and sociocultural adjustment in third culture kids: exploring the mediating roles of resilience and family functioning
Authors: Jones Emma E. , Reed Marnie , Meyer Andrea H. , Gaab Jens , Ooi Yoon P.
Published: August 2023

Introduction

This cross-sectional study explores the contributions of personal and contextual factors in the adjustment process of a sample of internationally mobile children and adolescents having relocated to Switzerland. Based on evolutionary developmental theories and recommendations by Research Domain Criteria and The Hierarchical Taxonomy of Psychopathology theoretical frameworks, we hypothesized and tested a heuristic model of TCK adjustment, aiming to identify prevention and treatment targets tailored for our sampled population.

Methods

We assessed the relationships in the hypothesized models, particularly how perceived and acculturative stress influence TCK adjustment and whether the relationship between the predictors of TCK stress and the outcomes of TCK adjustment are mediated by resilience and family functioning. A total of 143 participants aged 7–17, having relocated internationally with their working parent(s), recruited in local and international schools in Switzerland, were included in this study. Data were collected using an online survey after we collected consent. We assessed factors of adjustment using validated questionnaires: perceived stress and acculturative stress and the potential mediating roles of family functioning and resilience. We measured the outcome of adjustment through mental health difficulties and sociocultural adjustment. We used path analysis to test the model.

Results

Results highlight the contributions of perceived stress and acculturative stress to TCK mental health and sociocultural adjustment. We also we found a mediation effect for resilience in the relationship between perceived stress and mental health. Family functioning was not a significant mediator in any relationship that we assessed.

Discussion

We discuss implications for future research, promoting TCK adjustment and preventative psychotherapeutic interventions.
Translation and cross-cultural adaptation of the functional mobility scale in children with cerebral palsy into Arabic
Authors: Albalwi Abdulaziz A. , Saleh Maysoun N. , Alharbi Ahmad A. , Al-Bakri Qais , Alatawi Salem F.
Published: August 2023

Introduction

Cerebral palsy (CP) is a lifelong disorder of posture and movement which often leads to a myriad of limitations in functional mobility. The Functional Mobility Scale (FMS) is a parent-report measure of functional mobility for children with CP at three different distances (5 m, 50 m, and 500 m). This is a cross-sectional study which sought to translate and culturally adapt the FMS into Arabic and to validate the translated version. Functional mobility for children and adolescents with CP in Saudi Arabia was examined.

Methods

The translation methodology complied with the World Health Organization Disability Assessment Schedule 2.0 translation package. A total of 154 children with CP were recruited (mean age 8.16 ± 3.32 years). Parents were interviewed to rate the usual walking ability of their children on the Arabic FMS. The re-test assessment was done with 34 families. The mean time interval between the first and second sessions was 14.3 days (SD = 8.5), with a range of 6–37 days.

Results

Concurrent validity was explored using Spearman’s rank correlation coefficient between scores of the Arabic FMS with their corresponding score on the Gross Motor Function Classification System (GMFCS). Spearman’s r values ranged between (−0.895 and –0.779), indicating strong to very strong correlations. The Test–retest reliability was examined using Cohen’s weighted kappa, which showed almost perfect agreements. There was greater limitation for functional mobility at longer distances as 55.2% of children could not complete 500 meters (FMS score N). Overall, there was limited use of wheelchairs for all distances (ranging from 9.1% to 14.3%). Levels IV and V on the GMFCS had less variation in FMS scores and most of the children in these levels either did not complete the distances (no functional mobility at all distances) or used a wheelchair for mobility.

Discussion

The Arabic FMS was shown to be a reliable and valid measure of functional mobility for children with CP in their environment based on the parental reports. Functional mobility varied at different distances and within each GMFCS level. The use of both the GMFCS and FMS when assessing children with CP is recommended.
Spatial Characteristics of Transfer Plots and Conservation Tillage Technology Adoption: Evidence from a Survey of Four Provinces in China
Authors: Guo Yang , Cui Meiling , Xu Zhigang
Published: August 2023

Degradation in farmland quality owing to overuse emphasizes the current need for the adoption of protective technologies to ensure food security and sustainable resource utilization. This study employs plot survey data from Heilongjiang, Henan, Zhejiang, and Sichuan provinces in China to investigate how the spatial attributes of transferred plots influence the current adoption of farmland protection methods, such as deep tillage and straw-returning. Findings reveal that larger or interconnected transferred plots significantly increase the likelihood of farmers adopting conservation tillage technologies. However, the influence of the plot’s location on technology adoption varies among different plots. As the farmland transfer market expands, the spatial features of these plots emerge as critical determinants in the use of protective technologies. This underscores the pressing need for an integrated farmland transfer trading system and strengthened policy measures promoting land consolidation to foster widespread adoption of these conservation strategies.
A Co-Simulation Virtual Reality Machinery Simulator for Advanced Precision Agriculture Applications
Authors: Cutini Maurizio , Bisaglia Carlo , Brambilla Massimo , Bragaglio Andrea , Pallottino Federico , Assirelli Alberto , Romano Elio , Montaghi Alessandro , Leo Elisabetta , Pezzola Marco , Maroni Claudio , Menesatti Paolo
Published: August 2023

Simulation systems have become essential tools for both researchers and virtual laboratory experiments. In the Agri-food-chain, SimAgri, a driving simulator for tractors and operating machines, has been developed for precision agriculture (PA) research and to train professional farm drivers. Using the virtual environment of the simulator, the influence and fine-tuning of PA operations logic may be evaluated by simulating existing systems, or designing new ones, in specially compared scenarios and setups. Current configurations include an agricultural tractor carrying or towing farm equipment such as sprayers, seeders and fertilizer, embedded sensors, human–machine interfaces that may be configured like a joystick, console and touchscreen, and four virtual environment monitors. The study describes the design choices that have made it possible to create a simulator aimed at precision agriculture, keeping auto guidance, geolocation, and operations with ISOBUS implements as pillars. This research aims to use a unique purpose-designed simulation platform, installed on a driver-in-the-loop simulator to provide data to objectify the benefits of PA criteria. Numerical and experimental data have been compared to ensure results reliability.
The Effects of Localized Plant–Soil–Microbe Interactions on Soil Nitrogen Cycle in Maize Rhizosphere Soil under Long-Term Fertilizers
Authors: Li Yanan , Wang Chengyu , Wu Junnan , Zhang Yumang , Li Qi , Liu Shuxia , Gao Yunhang
Published: August 2023

Long-term fertilization can result in changes in the nitrogen (N) cycle in maize rhizosphere soil. However, there have been few reports on the impacts of plant–soil–microbe regulatory mechanisms on the N cycle in soil. In this study, soil samples were collected from a long-term experimental site located at Jilin Agricultural University, Changchun City, Jilin Province, Northeast China. We then analyzed the changes in the functional genes related to the N cycle, soil enzyme activity, and maize root exudates under long-term fertilizer application using metagenomics and liquid chromatography analysis. We aimed to investigate the response of the N cycle to long-term fertilizers, the interaction among plant, soil, and microbes, and the effect of the plant–soil–microbe system on the N cycle. Long-term fertilization had a significant effect on soil N contents, N2O emissions, and enzyme activity related to the N cycle in maize rhizosphere soil. The functional genes of the N cycle were mainly enriched in the N degradation pathway in maize rhizosphere soil. N fertilizer application decreased the abundance of functional genes related to N fixation and degradation, denitrification, and assimilatory nitrate reduction (ANRA) and increased the abundance of functional genes participating in dissimilatory nitrate reduction (DNRA) and anaerobic ammonia oxidation/hydroxylamine oxidation. The soil environment was positively related to soil enzyme activity and negatively related to the microbial community composition and amino acids in root exudates. The contribution rate of microorganisms to the N cycle was the highest (r2 = 0.900), followed by amino acids (r2 = 0.836) and the soil environment (r2 = 0.832). Therefore, we concluded that N fertilizer is the main factor limiting the soil N cycle and that microorganisms are the main factor regulating the N cycle in the plant–soil–microorganism system.
Establishment and Accuracy Evaluation of Cotton Leaf Chlorophyll Content Prediction Model Combined with Hyperspectral Image and Feature Variable Selection
Authors: Yu Siyao , Bu Haoran , Hu Xue , Dong Wancheng , Zhang Lixin
Published: August 2023

In order to explore the feasibility of rapid non-destructive detection of cotton leaf chlorophyll content during the growth stage, this study utilized hyperspectral technology combined with a feature variable selection method to conduct quantitative detection research. Through correlation spectroscopy (COS), a total of 882 representative samples from the seedling stage, bud stage, and flowering and boll stage were used for feature wavelength screening, resulting in 213 selected feature wavelengths. Based on all wavelengths and selected feature wavelengths, a backpropagation neural network (BPNN), a backpropagation neural network optimized by genetic algorithm (GA-BPNN), a backpropagation neural network optimized by particle swarm optimization (PSO-BPNN), and a backpropagation neural network optimized by sparrow search algorithm (SSA-BPNN) prediction models were established for cotton leaf chlorophyll content, and model performance comparisons were conducted. The research results indicate that the GA-BPNN, PSO-BPNN, and SSA-BPNN models established based on all wavelengths and selected feature wavelengths outperform the BPNN model in terms of performance. Among them, the SSA-BPNN model (referred to as COS-SSA-BPNN model) established using 213 feature wavelengths extracted through correlation analysis showed the best performance. Its determination coefficient and root-mean-square error for the prediction set were 0.920 and 3.26% respectively, with a relative analysis error of 3.524. In addition, the innovative introduction of orthogonal experiments validated the performance of the model, and the results indicated that the optimal solution for achieving the best model performance was the SSA-BPNN model built with 213 feature wavelengths extracted using the COS method. These findings indicate that the combination of hyperspectral data with the COS-SSA-BPNN model can effectively achieve quantitative detection of cotton leaf chlorophyll content. The results of this study provide technical support and reference for the development of low-cost cotton leaf chlorophyll content detection systems.
Isolation of Vascular Wall Mesenchymal Stem Cells from the Thoracic Aorta of Adult Göttingen Minipigs: A New Protocol for the Simultaneous Endothelial Cell Collection
Authors: Bernardini Chiara , Mantia Debora La , Salaroli Roberta , Ventrella Domenico , Elmi Alberto , Zannoni Augusta , Forni Monica
Published: August 2023

Two main classes of perivascular multipotent populations have been described: the microvascular pericytes and the vascular wall mesenchymal stem cells (VW-MSCs). VW-MSCs are isolated from large vessels in many species and they participate in vascular remodeling together with other cellular components such as endothelial cells. Considering that the Göttingen Minipigs are widely used in Europe as a translational model in the field of cardiovascular diseases, the aim of the present research was to isolate VW-MSCs from the adult aorta of Göttingen Minipigs while preserving and also collecting endothelial cells. The results obtained in the present research demonstrated that this new protocol allows us to obtain a pure population of VW-MSCs and endothelial cells. VW-MSCs from Göttingen Minipigs responded fully to the MSC minima international criteria, being positive to CD105, CD90, and CD44 and negative to CD45 and CD34. Moreover, VW-MSCs presented a differentiative potential towards osteogenic, chondrogenic, and adipogenic lineages. Overall, the present protocol, preserving the viability and phenotypic features of the two isolated populations, opens future possibilities of using minipig VW-MSCs and endothelial cells in in vitro vascular remodeling studies.
Sirtuin 1 Expression in Canine Mammary Tumors: A Pilot Study
Authors: Sgadari Mariafrancesca , Cacciola Nunzio Antonio , Power Karen , Martano Manuela , Restucci Brunella
Published: August 2023

Sirtuin 1 (SIRT1) is a protein involved in aging, cell protection, and energy metabolism in mammals. Recently, SIRT1 has been intensively studied in medical oncology, but the role of SIRT1 is still controversial, as it has been proposed as both an oncogene and a tumor suppressor. The aim of this study is to investigate the expression of SIRT1 by immunohistochemistry in canine mammary tissues, and by Western blot and immunofluorescence analysis in different canine mammary cell lines. Our results showed a decrease in SIRT1 expression from normal mammary gland tissue, and from benign and well-differentiated malignant tumors (G1) to less differentiated ones (G2–G3). Furthermore, a shift in the subcellular localization of SIRT1 from the nucleus to the cytoplasm was observed in less differentiated malignant tumors. However, further studies are needed to investigate the subcellular localization of SIRT1 in canine cancer cells and the role it may play in oncogenesis in animals.
Canine Babesiosis Caused by Large Babesia Species: Global Prevalence and Risk Factors—A Review
Authors: Zygner Wojciech , Gójska-Zygner Olga , Bartosik Justyna , Górski Paweł , Karabowicz Justyna , Kotomski Grzegorz , Norbury Luke J.
Published: August 2023

Canine babesiosis is a disease caused by protozoan pathogens belonging to the genus Babesia. Four species of large Babesia cause canine babesiosis (B. canis, B. rossi, B. vogeli, and the informally named B. coco). Although canine babesiosis has a worldwide distribution, different species occur in specific regions: B. rossi in sub-Saharan Africa, B. canis in Europe and Asia, and B. coco in the Eastern Atlantic United States, while B. vogeli occurs in Africa, southern parts of Europe and Asia, northern Australia, southern regions of North America, and in South America. B. vogeli is the most prevalent large Babesia species globally. This results from its wide range of monotropic vector species, the mild or subclinical nature of infections, and likely the longest evolutionary association with dogs. The most important risk factors for infection by large Babesia spp. include living in rural areas, kennels or animal shelters, or regions endemic for the infection, the season of the year (which is associated with increased tick activity), infestation with ticks, and lack of treatment with acaricides.
Identifying Migration Routes of Wild Asian Elephants in China Based on Ecological Networks Constructed by Circuit Theory Model
Authors: Jiang Xin , Liu Hong-Jie , Jiang Zhi-Yun , Ni Ru-Ping
Published: August 2023

Humans overlap with Asian elephants, resulting in frequent costly human–elephant conflicts, which disturb and even threaten local residents. In this study, we treat provincial and national nature reserves where Asian elephants still exist and other alternative habitats suitable for Asian elephants in southern Yunnan, China, as ecological patches. By using this approach, we can treat the terrain and surface state factors that hinder the migration of Asian elephants as a form of ecological resistance surface. We can then use a circuit theory model and remote sensing data to construct an ecological network, which allows us to identify ecological corridors and ecological pinch points. Herein, the possible migration routes of wild Asian elephants were identified. The main results are as follows: (1) In the study area, dense forests with steep slopes and high altitudes, cultivated land, and building land have greater migration resistance, while the gently undulating shrubs, bamboo forests, and grasslands far away from the city have less migration resistance. (2) There are three ecological corridor groups in the study area, mainly composed of shrub and grassland. The ecological corridors identified in this paper are the most likely migration routes of wild Asian elephants in China, and areas with higher simulated current densities reflect a higher probability of Asian elephants passing through. (3) According to the analysis, the ecological pinch points in the study area are 602 km2 in total, and woodland and grassland account for 89.2% of the total ecological pinch area. The areas where the pinch points are located have a high probability of Asian elephants passing through and a narrow space. Our findings can provide suggestions and solutions for the current conservation of wild Asian elephant species, alleviate human–elephant conflicts, promote the harmonious coexistence between humans and nature, and provide reference for biological protection and biological reserve planning.
A New Species of Demodex (Acariformes: Prostigmata) Observed in the Mouflon, Ovis aries musimon (Artiodactyla: Bovidae) with Data on the Parasitism and Occurrence of Other Ectoparasites
Authors: Kozina Paulina , Izdebska Joanna N. , Rolbiecki Leszek
Published: August 2023

A greater understanding of mite biodiversity and ecology can explain their preference for microhabitats within host bodies, i.e., as ecto-, meso-, and endoparasites. Similarly, learning about the patterns formed by mite communities in wild animals can shed light on the mechanisms of parasitosis development in their domesticated counterparts. Hence, the present study examined the acarofauna of the mouflon, introduced to Poland in the early 20th century from a region of endemic occurrence. Forty-one individuals were examined for the presence of ectoparasites between 2010 and 2013; later, skin fragments were analysed for the presence of skin mites. A new species of Demodecidae, Demodex musimonis sp. nov., was discovered in the skin of the pasterns of 14.6% mouflons, as well as the ectoparasitic tick Ixodes ricinus (prevalence 29.3%) and ungulate-typical fly Lipoptena cervi (34.1%). However, no mites typical for ovine Ovis (Psorergatidae, Psoroptidae) were noted, nor any colonisation of microhabitats by species from local ungulates, resulting from the loss of original parasites during the introduction. A comparison of the acarofauna of the mouflon and domestic sheep Ovis aries aries suggests that a mite community with a complex structure and the co-occurrence of different families may be formed. However, it is not known whether the acarofauna of domestic sheep is derived from wild ancestors or the process of domestication. Even so, the peculiar Demodecidae must have been part of the natural parasitofauna of wild sheep, of which only D. musimonis sp. nov. has been recognised so far.
Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress
Authors: Bittner-Schwerda Lilli , Herrera Carolina , Wyck Sarah , Malama Eleni , Wrenzycki Christine , Bollwein Heinrich
Published: August 2023

The extent of oxidative damage transferred by the damaged sperm to the progeny is likely to be limited by the oocyte’s repair and antioxidative capacity. We aimed to assess the association between Brilliant Cresyl Blue (BCB) staining in oocytes and their competence for embryo development after in vitro fertilisation (IVF) with damaged sperm. For this purpose, bovine sperm were incubated without (non-oxidised sperm, NOX S) or with 100 µM H2O2 (oxidised sperm, OX S) and were used to fertilise in-vitro-matured bovine oocytes (BCB-pos./BCB-neg.). Unstained oocytes served as controls (US). Development was assessed at 30, 46, 60 h and on Days (D) 7 and 8 after IVF. Total cell number and apoptotic index were analysed in D7 blastocysts. BCB-neg. oocytes showed lower cleavage rates and blastocyst rates than unstained oocytes after IVF with NOX S (p < 0.05). They showed the highest reduction in D7 blastocyst rate upon fertilisation with OX S and showed a delayed embryo development at 46 and 60 h after IVF compared to embryos produced with NOX S (p < 0.05). Total cell number in blastocysts produced with BCB-neg. oocytes was lower (p < 0.05) in the embryos produced with OX S than in embryos after IVF with NOX S. In conclusion, BCB-neg. oocytes have a lower competence to support embryo development after in vitro fertilisation with oxidised sperm.
Antibiotic Knowledge, Antibiotic Resistance Knowledge, and Antibiotic Use: A Cross-Sectional Study among Community Members of Bangkok in Thailand
Authors: Niyomyart Atsadaporn , Chow Susan Ka Yee , Bualoy Wunwisa , Butsing Nipaporn , Tao Xingjuan , Zhu Xuejiao
Published: August 2023

This study aimed to explore antibiotic knowledge, antibiotic resistance knowledge, and antibiotic use among adults in Bangkok, Thailand. This is a secondary analysis of cross-sectional data generated from a sample of 161 individuals living in Bangkok. Participants completed an online self-administered questionnaire developed by the World Health Organization. Descriptive analysis, the chi-square test, and multiple logistic regression analyses were performed. The sample comprised more females (56.5%) than males (42.2%). The majority of responders (67.7%) were between the ages of 18 and 40. More than half of the respondents mistakenly believed that antibiotics could treat colds and flu (54.7% and 47.2%, respectively). About 54.7% were aware that antibiotic resistance could harm them and their families. The chi-square test results showed that the levels of education were associated with antibiotic knowledge (p = 0.012), antibiotic resistance knowledge (p < 0.001), and antibiotic use (p = 0.023). Multiple logistic regressions showed that respondents with at least a bachelor’s degree or higher had better knowledge of antibiotics. Respondents who worked in the profession had better knowledge of antibiotic resistance. Respondents with sufficient incomes were more likely to use antibiotics. Baseline data from the study will be useful in antibiotic stewardship and public health campaigns among Bangkok residents.
Microbiota and Resistome Analysis of Colostrum and Milk from Dairy Cows Treated with and without Dry Cow Therapies
Authors: Patangia Dhrati V. , Grimaud Ghjuvan , Linehan Kevin , Ross R. Paul , Stanton Catherine
Published: August 2023

This study investigated the longitudinal impact of methods for the drying off of cows with and without dry cow therapy (DCT) on the microbiota and resistome profile in colostrum and milk samples from cows. Three groups of healthy dairy cows (n = 24) with different antibiotic treatments during DCT were studied. Colostrum and milk samples from Month 0 (M0), 2 (M2), 4 (M4) and 6 (M6) were analysed using whole-genome shotgun-sequencing. The microbial diversity from antibiotic-treated groups was different and higher than that of the non-antibiotic group. This difference was more evident in milk compared to colostrum, with increasing diversity seen only in antibiotic-treated groups. The microbiome of antibiotic-treated groups clustered separately from the non-antibiotic group at M2-, M4- and M6 milk samples, showing the effect of antibiotic treatment on between-group (beta) diversity. The non-antibiotic group did not show a high relative abundance of mastitis-causing pathogens during early lactation and was more associated with genera such as Psychrobacter, Serratia, Gordonibacter and Brevibacterium. A high relative abundance of antibiotic resistance genes (ARGs) was observed in the milk of antibiotic-treated groups with the Cephaguard group showing a significantly high abundance of genes conferring resistance to cephalosporin, aminoglycoside and penam classes. The data support the use of non-antibiotic alternatives for drying off in cows.
Fibrosis Development Linked to Alterations in Glucose and Energy Metabolism and Prooxidant–Antioxidant Balance in Experimental Models of Liver Injury
Authors: Semenovich Dmitry S. , Andrianova Nadezda V. , Zorova Ljubava D. , Pevzner Irina B. , Abramicheva Polina A. , Elchaninov Andrey V. , Markova Olga V. , Petrukhina Aleksandra S. , Zorov Dmitry B. , Plotnikov Egor Y.
Published: August 2023

The development of liver fibrosis is one of the most severe and life-threatening outcomes of chronic liver disease (CLD). For targeted therapy of CLD, it is highly needed to reveal molecular targets for normalizing metabolic processes impaired in damaged liver and associated with fibrosis. In this study, we investigated the morphological and biochemical changes in rat liver models of fibrosis induced by chronic administration of thioacetamide, carbon tetrachloride, bile duct ligation (BDL), and ischemia/reperfusion (I/R), with a specific focus on carbohydrate and energy metabolism. Changes in the levels of substrates and products, as well as enzyme activities of the major glucose metabolic pathways (glycolysis, glucuronidation, and pentose phosphate pathway) were examined in rat liver tissue after injury. We examined key markers of oxidative energy metabolism, such as the activity of the Krebs cycle enzymes, and assessed mitochondrial respiratory activity. In addition, pro- and anti-oxidative status was assessed in fibrotic liver tissue. We found that 6 weeks of exposure to thioacetamide, carbon tetrachloride, BDL or I/R resulted in a decrease in the activity of glycolytic enzymes, retardation of mitochondrial respiration, elevation of glucuronidation, and activation of pentose phosphate pathways, accompanied by a decrease in antioxidant activity and the onset of oxidative stress in rat liver. Resemblance and differences in the changes in the fibrosis models used are described, including energy metabolism alterations and antioxidant status in the used fibrosis models. The least pronounced changes in glucose metabolism and mitochondrial functions in the I/R and thioacetamide models were associated with the least advanced fibrosis. Ultimately, liver fibrosis significantly altered the metabolic profile in liver tissue and the flux of glucose metabolic pathways, which could be the basis for targeted therapy of liver fibrosis in CLD caused by toxic, cholestatic, or I/R liver injury.