bluuebunny/crossref_metadata_2025 huggingface dataset:
Urinary trypsinogen activation peptide as a marker of severe acute pancreatitis
Authors: Johnson C D , Lempinen M , Imrie C W , Puolakkainen P , Kemppainen E , Carter R , McKay C
Published: June 2004

Background

Trypsinogen activation peptide (TAP) may be an early marker of severe pancreatitis. Previous studies have included all patients with organ failure in the group with severe pancreatitis, although patients with transient organ failure may have a good prognosis. The aim of this study was to determine the value of urinary TAP estimation for prediction of severity of acute pancreatitis, and to validate use of several markers of prediction of severity against a new, stringent definition of severity.

Methods

Patients with acute pancreatitis were recruited within 24 h of onset of symptoms. Urine and blood samples were collected for 24 h, and Acute Physiology And Chronic Health Evaluation (APACHE) II (24 h), Ranson (48 h) and Glasgow (48 h) scores were calculated. Severe acute pancreatitis was defined by the presence of a local complication or the presence of organ failure for more than 48 h.

Results

Urinary TAP levels were significantly greater in patients with severe pancreatitis than in those with mild disease during the first 36 h of admission. The highest of three estimations of TAP in the first 24 h was as effective as APACHE II at 24 h in predicting severity. At 24 h after admission, urinary TAP was better than C-reactive protein (CRP) in predicting severity. The combination of TAP and CRP at 24 h allowed identification of high- and low-risk groups. The new definition of severity excluded 24 of 190 patients with transient organ failure; none of these patients died.

Conclusion

Use of TAP improved early prediction of the severity of acute pancreatitis. Organ failure that resolves within 48 h does not signify a severe attack of acute pancreatitis.
Laparoscopically assisted distal gastrectomy for early gastric cancer in the elderly
Authors: Yasuda K , Sonoda K , Shiroshita H , Inomata M , Shiraishi N , Kitano S
Published: June 2004

Background

Open gastrectomy is associated with increased morbidity and a longer hospital stay than laparoscopically assisted gastrectomy. The aim of this study was to clarify the value of laparoscopically assisted distal gastrectomy (LDG) in the elderly, in whom co-morbid disease is generally more common.

Methods

Forty-five elderly patients (aged 70 years or more) and 57 younger patients who underwent LDG, and 28 elderly patients who underwent open distal gastrectomy (ODG) for early gastric cancer between January 1994 and April 2003 were studied. Demographics and postoperative outcomes were compared.

Results

Co-morbidity was more common in elderly patients than in younger patients who underwent LDG (25 of 45 versus 16 of 57; P = 0·004). The postoperative complication rate, time to solid diet and postoperative hospital stay were similar in these two groups. Elderly patients who underwent LDG had a significantly reduced medical complication rate (two of 45 versus six of 28; P = 0·023), time to first flatus (3·7 versus 4·2 days; P = 0·042), time to solid diet (4·6 versus 5·5 days; P = 0·011) and postoperative hospital stay (16·3 versus 23·9 days; P = 0·011) than elderly patients who had ODG.

Conclusion

LDG offers particular advantages to elderly patients with early gastric cancer, including rapid return of gastrointestinal function, fewer complications and a shorter hospital stay.
Experimental and Theoretical Investigation on the Relative Stability of the PdS2‐ and Pyrite‐Type Structures of PdSe2.
Authors: Soulard C. , Rocquefelte X. , Petit P.‐E. , Evain M. , Jobic S. , Itle J.‐P. , Munsch P. , Koo H.‐J. , Whangbo M.‐H.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Synthesis and Crystal Structure of Pb3O2(SeO3).
Authors: Krivovichev Sergey V. , Avdontseva Evgeniya Yu. , Burns Peter C.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Yb8Ge3Sb5, a Metallic Mixed‐Valent Zintl Phase Containing the Polymeric 1∞[Ge4‐3] Anions.
Authors: Salvador James R. , Bilc Daniel , Mahanti S. D. , Hogan Tim , Guo Fu , Kanatzidis Mercouri G.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
The Crystal Structure of ζ1‐Al3Cu4
Authors: Gulay L. D. , Harbrecht B.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Crystal Structure of the Ternary Silicide CeRh2Si
Authors: Tursina A. I. , Gribanov A. V. , Seropegin Y. D. , Novitskii A. A. , Bodak O. I.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Thermochemistry of Gaseous Species in the Dy—Br and Dy—I Systems
Authors: Hildenbrand D. L. , Lau K. H. , Baglio J. W. , Struck C. W.
Published: June 2004

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Phase Equilibria and Crystal Structures of the Compounds in the Zr—Li—{Ge, Pb} Systems at 470 K.
Authors: Zatorska G. M. , Pavlyuk V. V. , Davydov V. M.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
New Solid—Gas Metathetical Synthesis of Binary Metal Polysulfides and Sulfides at Intermediate Temperatures: Utilization of Boron Sulfides.
Authors: Wu Li‐Ming , Seo Dong‐Kyun
Published: June 2004

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Ambient Pressure Synthesis of Corundum‐Type In2O3.
Authors: Epifani Mauro , Siciliano Pietro , Gurlo Alexander , Barsan Nicolae , Weimar Udo
Published: June 2004

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A Polyoxometalate Containing the {Ni2N3} Fragment: Ferromagnetic Coupling in a NiII μ‐1,1 Azido Complex with a Large Bridging Angle.
Authors: Mialane Pierre , Dolbecq Anne , Riviere Eric , Marrot Jerome , Secheresse Francis
Published: June 2004

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Synthesis, Crystal Structures, and Nonlinear Optical Properties of Two Cubane‐Like Cluster Compounds [(n‐Bu)4N]3 [MoS4Ag3Cl4] and [Et4N]3[WOS3Cu3I4].
Authors: Zhou Jian‐Liang , Song Ying‐Lin , Li Yi‐Zhi , Zheng He‐Gen , Xin Xin‐Quan
Published: June 2004

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New Dihydroxy Bis(oxazoline) Ligands for the Palladium‐Catalyzed Asymmetric Allylic Alkylation: Experimental Investigations of the Origin of the Reversal of the Enantioselectivity.
Authors: Ait‐Haddou Hassan , Hoarau Olivier , Cramailere Dimitri , Pezet Frederic , Daran Jean‐Claude , Balavoine Gilbert G. A.
Published: June 2004

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Novel Planar Chiral Diphosphines and Their Application in Asymmetric Hydrogenations and Asymmetric Heck Reactions.
Authors: Gibson Susan E. , Ibrahim Hasim , Pasquier Corinne , Swamy Vishwanath M.
Published: June 2004

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Highly Enantioselective Asymmetric Hydrogenation of α‐Phthalimide Ketone: An Efficient Entry to Enantiomerically Pure Amino Alcohols.
Authors: Lei Aiwen , Wu Shulin , He Minsheng , Zhang Xumu
Published: June 2004

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Readily Available [2.2.2]‐Bicyclooctadienes as New Chiral Ligands for Ir(I): Catalytic, Kinetic Resolution of Allyl Carbonates.
Authors: Fischer Christian , Defieber Christian , Suzuki Takeyuki , Carreira Erick M.
Published: June 2004

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Desymmetrization of N‐Sulfonated Aziridines by Alkyllithium Reagents in the Presence of Chiral Ligands.
Authors: Mueller Paul , Riegert David , Bernardinelli Gerald
Published: June 2004

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Diastereoselective Baylis—Hillman Reaction Using N‐Glyoxyloyl Camphorpyrazolidinone as an Electrophile: Synthesis of Optically Pure 2‐Hydroxy‐3‐methylene Succinic Acid Derivative.
Authors: Pan Jia‐Fu , Chen Kwunmin
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Palladium‐Catalyzed Addition of Disulfides and Diselenides to Alkynes under Solvent Free Conditions.
Authors: Ananikov Valentine P. , Beletskaya Irina P.
Published: June 2004

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Boron Trifluoride Catalyzed Reaction of Alkyl Fluoride with Silyl Enolate, Allylsilane, and Hydrosilane.
Authors: Hirano Koji , Fujita Kazuya , Yorimitsu Hideki , Shinokubo Hiroshi , Oshima Koichiro
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Polyheterocyclic Systems Incorporating Pyrazole, Thiophene, Thiazole, and Thiadiazole Moieties.
Authors: Dawood Kamal M. , Ragab Eman A. , Farag Ahmad M.
Published: June 2004

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Convenient Switching of 7‐endo/6‐exo Radical Cyclization.
Authors: Kamimura Akio , Taguchi Yohei
Published: June 2004

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Selective Electrochemical Aromatic Fluorination of Acetophenone and Benzophenone.
Authors: Shainyan B. A. , Danilevich Yu. S. , Grigor'eva A. A. , Chuvashev Yu. A.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
“Acetylene Zipper” Reactions and Pd—Cu‐Catalyzed Cross‐Coupling in the Synthesis of Vicinal 1,3‐Alkadiynylarylamines and Aminopyridines.
Authors: Balova I. A. , Morozkina S. N. , Sorokoumov V. N. , Vinogradova O. V. , Vasilevskii S. F.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Lewis Acid Catalysis in Supercritical Carbon Dioxide. Use of Poly(ethylene glycol) Derivatives and Perfluoroalkylbenzenes as Surfactant Molecules which Enable Efficient Catalysis in scCO2.
Authors: Komoto Ichiro , Kobayashi Shu
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
α‐Sulfinyl Carbanions as a New Source of Olefins.
Authors: Abramovitch Adi , Varghese Jos P. , Marek Ilan
Published: June 2004

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Reactions of a Carbamoylstannane with Acid Chlorides: Highly Efficient Synthesis of α‐Oxo Amides.
Authors: Hua Ruimao , Takeda Hide‐aki , Abe Yoshimoto , Tanaka Masato
Published: June 2004

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Indium‐Mediated Mild and Facile Method for the Synthesis of Amides.
Authors: Cho Dae Hyan , Jang Doo Ok
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
A New Heterobifunctional Reagent for Immobilization of Biomolecules on Glass Surface
Authors: Kumar P. , Agrawal S. K. , Misra A. , Gupta K. C.
Published: June 2004

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A Convenient and Stereoselective Method for the Preparation of 2‐Substituted 1,3‐Alkadienes from 1,2‐Disubstituted Cyclopropanols.
Authors: Kozyrkov Yurii Yu. , Kulinkovich Oleg G.
Published: June 2004

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A Direct Link Between the Passerini Reaction and α‐Lactams.
Authors: Lengyel Istvan , Cesare Victor , Taldone Tony
Published: June 2004

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Bicyclo[3.2.1]octane Synthons from Cyclopropenes: Functionalization of Cycloadducts by Nucleophilic Additions.
Authors: Orugunty Ravi S. , Wright Dennis L. , Battiste Merle A. , Helmich Richard J. , Abboud Khalil
Published: June 2004

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Pseudo‐C3‐Symmetric Trisoxazolines as Ligands in Copper Catalyzed Enantioselective Diels—Alder Reaction.
Authors: Zhou Jian , Tang Yong
Published: June 2004

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Modified Synthetic Route to 2‐Hydroxyimino‐N‐arylacetamides.
Authors: Krapcho A. Paul , Cadamuro Sergio A.
Published: June 2004

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Metalation of α‐Diazocarbonyl Compounds Using Grignard Reagents. A Convenient Synthesis of α‐Diazo‐β‐ketoesters and Mixed Esters of α‐Diazomalonate.
Authors: Cuevas‐Yanez Erick , Muchowski Joseph M. , Cruz‐Alamanza Raymundo
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Oxazolidine Ring Opening and Isomerization to (E)‐Imines. Asymmetric Synthesis of Aryl‐α‐fluoroalkyl Amino Alcohols.
Authors: Gosselin Francis , Roy Amelie , O'Shea Paul D. , Chen Cheng‐yi , Volante Ralph P.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Highly Efficient Aza‐Baylis—Hillman Reaction of N‐Tosylated Imines with MVK, Acrolein, and Phenyl Acrylate or α‐Naphthyl Acrylate: Lewis Base Effects and A Convenient Method to Synthesize α,β‐Unsaturated β‐Amino Carbonyl Compounds.
Authors: Xu Yong‐Mei , Shi Min
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Solvent‐Free Benzylic Oxidations Using Urea‐Hydrogen Peroxide Complex (UHP) under Microwave Irradiation.
Authors: Paul Satya , Nanda Puja , Gupta Rajive
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Studies on Novel Synthetic Methodologies. Part 34. Convenient and Efficient Stereoselective Synthesis of (2Z)‐2‐(Chloromethyl)alk‐2‐enoates Using Iron(III) or Indium(III) Chloride.
Authors: Das Biswanath , Banerjee Joydeep , Ravindranath Nasi , Venkataiah Bollu
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Transalkenylation of Geminally Activated Nitroethenes.
Authors: Aboskalova N. I. , Fel'gendler A. V. , Sheremet E. A. , Trukhin E. V. , Berestovitskaya V. M.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Disulfidation of Alkynes and Alkenes with Gallium Trichloride.
Authors: Usugi Shin‐ichi , Yorimitsu Hideki , Shinokubo Hiroshi , Oshima Koichiro
Published: June 2004

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Efficient Synthesis of Polysubstituted Acylguanidines and Guanylureas.
Authors: Katritzky Alan R. , Rogovoy Boris V. , Cai Xiaohong , Kirichenko Nataliya , Kovalenko Katherine V.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Oxime Carbapalladacycle Covalently Anchored to High Surface Area Inorganic Supports or Polymers as Heterogeneous Green Catalysts for the Suzuki Reaction in Water.
Authors: Baleizao Carlos , Corma Avelino , Garcia Hermenegildo , Leyva Antonio
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Synthesis of Symmetric 1,3,5‐Triarylbenzenes.
Authors: Hu Zhi Guo , Liu Jun , Li Gong An , Dong Zhi Bing
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Selenobenzophenones and Diazoalkanes: Isolation of Tetraarylethylenes by the Reaction of Benzophenone Hydrazones with Diselenium Dibromide.
Authors: Okuma Kentaro , Kojima Kazuki , Oyama Kosuke , Kubo Kento , Shioji Kosei
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
A Novel Method for the Preparation of Benzylidenecyclohexanes with High Optical Purity.
Authors: Nakamura Shuichi , Ogura Takahiro , Wang Libo , Toru Takeshi
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Nucleophilic Partners in the Tandem Conjugate Addition—Dieckmann Condensation Reaction. Part 1. Synthesis of 1,2,3‐Trisubstituted Naphthalenes.
Authors: Martinez Aaron D. , Deville Jay P. , Stevens Joel L. , Behar Victor
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Synthesis of Alkylated Aminofluorenes by Palladium‐Catalyzed Substitution at Halofluorenes.
Authors: Saroja Ginagunta , Pingzhu Zhang , Ernsting Nikolaus P. , Liebscher Juergen
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
An Efficient Synthesis of Substituted Anthraquinones and Naphthoquinones.
Authors: Bailey David , Williams Vance E.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Overcrowded 5,10,15‐Trisubstituted Derivatives: Synthesis of 5,10,15‐Tri(fluorenylidene)truxene.
Authors: Ruiz Marta , Gomez‐Lor Berta , Santos Amelia , Echavarren Antonio M.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
The Broensted Acid Catalyzed Direct Aza‐Darzens Synthesis of N‐Alkyl cis‐Aziridines.
Authors: Williams Amie L. , Johnston Jeffrey N.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Stereoselective Synthesis of Tetrahydrofurans via the Palladium‐Catalyzed Reaction of Aryl Bromides with γ‐Hydroxy Alkenes: Evidence for an Unusual Intramolecular Olefin Insertion into a Pd(Ar)(OR) Intermediate.
Authors: Wolfe John P. , Rossi Michael A.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Cation Radical [3 + 2] Cycloaddition of Chalcone Epoxides: A Facile Synthesis of Highly Substituted Tetrahydrofurans.
Authors: Huo Congde , Jia Xiaodong , Zhang Wei , Yang Li , Lue Jianming , Liu Zhong‐Li
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
A Convenient Approach to the Synthesis of Fused Acetal Derivatives by Tetrabutylammonium Peroxydisulfate‐Mediated Oxidative Cycloaddition of 1,3‐Dicarbonyl Compounds to Cyclic Enol Ethers: A Rebuttal.
Authors: Jadhav Vidyadhar , Park Min Young , Kim Yong Hae
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Captodative Olefins: Methyl 2‐Aryloxy‐3‐dimethylaminopropenoates and Their Application in a New Synthesis of Benzofurans.
Authors: del Carmen Cruz Maria , Tamariz Joaquin
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Preparation and Characterization of 3,4‐Diaryl‐Substituted 2‐Diarylaminothiophenes.
Authors: Zeika Olaf , Hartmann Horst
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Synthesis and X‐Ray Diffraction Study of 2‐(2‐Acyl‐1‐phenylethenyl)pyrroles.
Authors: Stepanova Z. V. , Sobenina L. N. , Mikhaleva A. I. , Ushakov I. A. , Elokhina V. N. , Vorontsov I. I. , Antipin M. Yu. , Trofimov B. A.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Fluoropyrrolidine Amides as Dipeptidyl Peptidase IV Inhibitors.
Authors: Caldwell Charles G. , et al. et al.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
A New Series of Potent Oxindole Inhibitors of CDK2.
Authors: Luk Kin‐Chun , Simcox Mary Ellen , Schutt Andy , Rowan Karen , Thompson Thelma , Chen Yi , Kammlott Ursula , DePinto Wanda , Dunten Pete , Dermatakis Apos
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Ionic Liquid as an Efficient Promoting Medium for Fixation of CO2: Clean Synthesis of α‐Methylene Cyclic Carbonates from CO2 and Propargyl Alcohols Catalyzed by Metal Salts under Mild Conditions.
Authors: Gu Yanlong , Shi Feng , Deng Youquan
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Mild Conditions for Copper‐Catalyzed N‐Arylation of Pyrazoles.
Authors: Cristau Henri‐Jean , Cellier Pascal P. , Spindler Jean‐Francis , Taillefer Marc
Published: June 2004

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Synthesis and in vitro Cytotoxicity of 5‐Substituted 2‐Cyanoimino‐4‐imidazodinone and 2‐Cyanoimino‐4‐pyrimidinone Derivatives.
Authors: Chern Jyh‐Haur , Shia Kak‐Shan , Chang Chung‐Ming , Lee Chung‐Chi , Lee Yen‐Chun , Tai Chia‐Liang , Lin Ying‐Ting , Chang Chih‐Shiang , Tseng Huan‐Yi
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Benzimidazolone p38 Inhibitors.
Authors: McClure Kim F. , et al. et al.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Ring Opening Reactions of N‐Alkyl Oxazolidinones with Organolithium Reagents.
Authors: Jones Simon , Norton Helen C.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Facile Syntheses of Oxazolines and Thiazolines with N‐Acylbenzotriazoles under Microwave Irradiation.
Authors: Katritzky Alan R. , Cai Chunming , Suzuki Kazuyuki , Singh Sandeep K.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Design and Synthesis of Benzoxazole Derivatives as Novel Melatoninergic Ligands.
Authors: Sun Li‐Qiang , Chen Jie , Takaki Katherine , Johnson Graham , Iben Lawrence , Mahle Cathy D. , Ryan Elaine , Xu Cen
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Microwave‐Assisted Synthesis of Sterically Hindered 3‐(5‐Tetrazolyl)pyridines.
Authors: Bliznets Igor V. , Vasil'ev Andrei A. , Shorshnev Sergey V. , Stepanov Aleksandr E. , Lukyanov Sergey M.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
CAN‐Mediated Cyclization of Epoxypropyl Cinnamyl Ethers: A Facile Stereoselective Synthesis of Tetrahydropyran Derivatives.
Authors: Nair Vijay , Balagopal Lakshmi , Rajan Roshini , Deepthi Ani , Mohanan Kishor , Rath Nigam P.
Published: June 2004

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A Novel and Direct Synthesis of Chroman Derivatives Using a Hypervalent Iodine(III) Reagent.
Authors: Hamamoto Hiromi , Hata Kayoko , Nambu Hisanori , Shiozaki Yukiko , Tohma Hirofumi , Kita Yasuyuki
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Antagonists of Human CCR5 Receptor Containing 4‐(Pyrazolyl)piperidine Side Chains. Part 1. Discovery and SAR Study of 4‐Pyrazolylpiperidine Side Chains.
Authors: Shen Dong‐Ming , et al. et al.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Synthesis of 2‐(2‐Naphthyl)quinolines from Z‐3‐(2‐Naphthyl)‐3‐chloro‐2‐propenal.
Authors: Vashkevich E. V. , Potkin V. I. , Kozlov N. G. , Skakovskii E. D.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Orally Active Antiproliferation Agents: Novel Diphenylamine Derivatives as FGF‐R2 Autophosphorylation Inhibitors.
Authors: Shimizu Toshiyuki , et al. et al.
Published: June 2004

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Design and Synthesis of 3,7‐Diarylimidazopyridines as Inhibitors of the VEGF‐Receptor KDR.
Authors: Wu Zhicai , et al. et al.
Published: June 2004

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Synthesis of Functionalized 7‐Azaindoles via Directed ortho‐Metalations.
Authors: L'Heureux Alexandre , Thibault Carl , Ruel Rejean
Published: June 2004

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Synthesis and Functionalization of 1H‐Pyrazolo[3,4‐b]pyridines Involving Copper and Palladium‐Promoted Coupling Reactions.
Authors: Lavecchia G. , Berteina‐Raboin S. , Guillaumet G.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
A Facile Route to Pyrrolo[3′,4′:3,4]pyrido[1,2‐a]benzimidazoles: Novel Heterocyclic Derivatives.
Authors: Tereshchenko Alexander D. , Tolmachev Andrey A. , Tverdokhlebov Anton V.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
A Facile Synthesis of Novel 1,7‐Dihydropyrazolo[3,4‐b]pyridine‐4,6‐diones.
Authors: Yuan Dekai , Li Zhengming , Zhao Weiguang
Published: June 2004

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A Facile Synthesis of 6‐Aryl‐1‐methyl‐3‐n‐propyl‐6,7‐dihydro‐1H‐pyrazolo [4,3‐d]pyrimidin‐7‐ones.
Authors: Reddy N. Ram , Reddy G. Mahesh , Reddy P. Pratap
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Macrocyclic Piperazinones as Potent Dual Inhibitors of Farnesyltransferase and Geranylgeranyltransferase‐I.
Authors: Dinsmore Christopher J. , et al. et al.
Published: June 2004

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2,3‐Disubstituted Pyrrolo[2,3‐b]quinoxalines via Aminopalladation — Reductive Elimination.
Authors: Arcadi Antonio , Cacchi Sandro , Fabrizi Giancarlo , Parisi Luca M.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Palladium‐Catalyzed Regiospecific Tandem Allylation of 2‐Aminophenols Using 2‐Butene‐1,4‐diol.
Authors: Yang Shyh‐Chyun , Lai Hwe‐Chen , Tsai Yan‐Chiu
Published: June 2004

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Synthesis and Antitumor Activity of s‐Tetrazine Derivatives.
Authors: Hu Wei‐Xiao , Rao Guo‐Wu , Sun Ya‐Quan
Published: June 2004

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A Useful 8‐Hydroxyquinoline Synthon.
Authors: Cross Jason P. , Sammes Peter G.
Published: June 2004

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Stereoselective Synthesis of 1,4‐Dienylsilanes via Hydromagnesiation of Alkynylsilanes.
Authors: Zhao Hong , Hao Wenyan , Cai Mingzhong , Zhou Zhou
Published: June 2004

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Selective Preparation of 1,3‐Butadienyl Phosphines, 1‐Iodo‐ and 1,4‐Diiodo‐butadienyl Phosphine Oxides via Zirconocene‐Mediated Cross‐Coupling of Alkynylphosphines.
Authors: Xi Zhenfeng , Zhang Wenxiong , Takahashi Tamotsu
Published: June 2004

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Convenient Synthesis of 1‐Aminoalkylphosphonates under Solvent‐Free Conditions.
Authors: Kaboudin Babak , Rahmani Abbas
Published: June 2004

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General Synthesis of Unsymmetrical Norbornane Scaffolds as Inducers for Hydrogen Bond Interactions in Peptides.
Authors: Hackenberger Christian P. R. , Schiffers Ingo , Runsink Jan , Bolm Carsten
Published: June 2004

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Efficient Synthesis of O‐, S‐, N‐ and C‐Glycosides of 2‐Amino‐2‐deoxy‐D‐glucopyranose from Glycosyl Iodides.
Authors: Miquel Nicolas , Vignando Stefano , Russo Giovanni , Lay Luigi
Published: June 2004

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Highly Stereoselective Synthesis of Aristeromycin Through Dihydroxylation of 4‐Aryl‐1‐azido‐2‐cyclopentenes.
Authors: Ainai Takauki , Wang Yong‐Gang , Tokoro Yuko , Kobayashi Yuichi
Published: June 2004

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Synthesis of New Halogenated Flavonoids by Reaction with Hydrogen Halides in the Presence of Dimethyldioxirane.
Authors: Arnone Alberto , Nasini Gianluca , Panzeri Walter , de Pava Orso Vajna , Zucca Cristina
Published: June 2004

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A Facile Synthesis of Dihydronaphthopyrans.
Authors: Ranade Anup A. , Joseph Augustine R. , Kumbhar Virendra B. , Paradkar Madhusudan V.
Published: June 2004

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Benzylation of Flavan‐3‐ols (Cathechins).
Authors: Mustafa Jamal , Khan Riaz , Khan Ikhlas A. , Ferreira Daneel
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Effective Irreversible Alkylating Reagents Based on the Structure of Clavulones.
Authors: Tanaka Hiroshi , Kitade Makoto , Iwashima Makoto , Iguchi Kazuo , Takahashi Takashi
Published: June 2004

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Biosynthetic Capacities of Actinomycetes. Part 30. Lactonamycin Z, an Antibiotic and Antitumor Compound Produced by Streptomyces sanglieri Strain AK 623.
Authors: Hoeltzel Alexandra , Dieter Anke , Schmid Dietmar G. , Brown Rose , Goodfellow Michael , Beil Winfried , Jung Guenther , Fiedler Hans‐Peter
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Isolation, Structure and HIV‐1 Integrase Inhibitory Activity of Exophillic Acid, a Novel Fungal Metabolite from Exophiala pisciphila.
Authors: Ondeyka John G. , Zink Deborah L. , Dombrowski Anne W. , Polishook Jon D. , Felock Peter J. , Hazuda Daria J. , Singh Sheo B.
Published: June 2004

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Stereodivergent and Reiterative Synthesis of Bistetrahydrofuran Ring Cores of Annonaceous Acetogenins.
Authors: Kojima Naoto , Maezaki Naoyoshi , Tominaga Hiroaki , Yanai Minori , Urabe Daisuke , Tanaka Tetsuaki
Published: June 2004

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Large‐Scale Synthesis of Uniform Nanotubes of a Nickel Complex by a Solution Chemical Route.
Authors: Guo Lin , Liu Chenmin , Wang Rongming , Xu Huibin , Wu Ziyu , Yang Shihe
Published: June 2004

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New Derivatives of 4‐Aminobicyclo[2.2.2]octanones and ‐ols as Potential Antiprotozoals.
Authors: Seebacher Werner , Brun Reto , Saf Robert , Kroepfl Dietmar , Weis Robert
Published: June 2004

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Engineering of Solid State Ionic Devices
Authors: Weppner Werner
Published: June 2004

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Ultrafast Vibrational Population Dynamics of Water and Related Systems: A Theoretical Perspective
Authors: Rey Rossend , Moeller Klaus B. , Hynes James T.
Published: June 2004

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Proceedings of the VI Latin American Workshop on Magnetism, Magnetic Materials and Their Applications
Authors: several authors several authors
Published: June 2004

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Beyond Crystallography: The Study of Disorder, Nanocrystallinity and Crystallographically Challenged Materials with Pair Distribution Functions
Authors: Billinge Simon J. L. , Kanatzidis M. G.
Published: June 2004

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Scorpionates: Genesis, Milestones, Prognosis
Authors: Trofimenko Swiatoslaw
Published: June 2004

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Why Fluorinate Fullerenes?
Authors: Taylor Roger
Published: June 2004

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Acetylation with FeCl3 as Catalyst
Authors: Schilling Gerhard
Published: June 2004

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Recent Developments in the Use of N‐Heterocyclic Carbenes: Applications in Catalysis
Authors: Kissling Rebecca M. , Viciu Mihai S. , Grasa Gabriela A. , Germaneau Romain F. , Gueveli Tatyana , Pasareanu Marie‐Christiane , Navarro‐Fernandez Oscar , Nolan Steven P.
Published: June 2004

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Hydrogenation Reactions in Supercritical CO2 Catalyzed by Metal Nanoparticles in a Water‐in‐Carbon Dioxide Microemulsion
Authors: Ohde Mariko , Ohde Hiroyuki , Wai Chien M.
Published: June 2004

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The Challenge of Simplexity. The Simple and the Complex in Organic Synthesis
Authors: Compain Philippe
Published: June 2004

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Electro‐Organic Synthesis without Supporting Electrolyte: Possibilities of Solid Polymer Electrolyte Technology
Authors: Joerissen J.
Published: June 2004

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Photoinduced Electron Transfer Dynamics in Aromatic Vinyl Polymers and Related Systems: Time‐Resolved Detection of Primary Events
Authors: Miyasaka Hiroshi , Khan Sazzadur R. , Itaya Akira
Published: June 2004

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Preparation of a Gel‐Forming Ointment Base Applicable to the Recovery Stage of Bedsore and Clinical Evaluation of a Treatment Method with Different Ointment Bases Suitable to each Stage of Bedsore
Authors: Shigeyama Masato
Published: June 2004

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Synthetic Methodology Utilized to Prepare Substituted Imidazole P38 MAP Kinase Inhibitors
Authors: Murry Jerry A.
Published: June 2004

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Natural Product‐Like and Other Biologically Active Heterocyclic Libraries Using Solid‐Phase Techniques in the Postgenomic Era
Authors: Knepper K. , Gil C. , Braese S.
Published: June 2004

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Total Synthesis Highlights
Authors: Allin Steven M. , Allard James E. , Khera Jagjit S. , Chauhan Ritesh
Published: June 2004

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The Future of Fatty Acid Research: Essential and Imaginative
Authors: Lie Ken Jie Marcel S. F. , Lam Corey N. W.
Published: June 2004

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Preparation of Water‐Soluble Compounds by Covalent Attachment of Solubilizing Moieties
Authors: Wermuth Camille G. , de La Fontaine Jeal
Published: June 2004

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Solid Dispersions: Revival with Greater Possibilities and Applications in Oral Drug Delivery
Authors: Sethia Sundeep , Squillante Emilio
Published: June 2004

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Advances in Reactive‐Transport Modeling of Contaminant Release and Attenuation from Mine‐Waste Deposits
Authors: Mayer K. U. , Blowes D. W. , Frind E. O.
Published: June 2004

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Organic Boronic Acids and Boronic Acid Esters in Industrial Synthesis
Authors: Zenk Roland , Partzsch Steffen
Published: June 2004

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Theoretical and Physical Aspects of Stepwise Mechanisms in Acyl‐Transfer Reactions
Authors: Lee Ikchoon , Sung Dae Dong
Published: June 2004

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Mass Spectral Fragmentation Reactions of 4‐Azasteroids
Authors: Burinsky D. , Williams J. , Thornquest A. Jr. , Sides S.
Published: June 2004

For Abstract see ChemInform Abstract in Full Text.
Polarographic Behavior of Gemfibrozil in the Presence of Dissolved Oxygen and Its Analytical Application
Authors: Xu Maotian , Song Junfeng , Guo Wei , Li Ning
Published: June 2004

The polarographic behavior of gemfibrozil is investigated in 0.2 mol L−1 KH2PO4‐Na2HPO4 (pH 5.8±0.1)‐8% ethanol supporting electrolyte in the absence and the presence of dissolved oxygen. The results demonstrate that the polarographic reduction peak at ca. −1.17 V is a catalytic hydrogen wave after deaeration, and the enhanced reduction peak in the presence of dissolved oxygen is the so‐called parallel catalytic hydrogen wave. Based on the parallel catalytic hydrogen wave, a novel method has been developed for the determination of gemfibrozil by single sweep polarography. Calibration plot is linear in the range of 1.8×10−7–2.4×10−6 mol L−1 and detection limit is 9.0×10−8 mol L−1. The proposed method is applied to the direct determination of the gemfibrozil in pharmaceutical preparations. The proposed method is simpler, faster and more sensitive than the known methods for gemfibrozil analyses.
Voltammetric Determination of Platinum in Plant Material
Authors: Kowalska Joanna , Huszal Sylwester , Sawicki Marek G. , Asztemborska Monika , Stryjewska Ewa , Szalacha Elzbieta , Golimowski Jerzy , Gawronski Stanislaw W.
Published: June 2004

A very sensitive procedure for the determination of platinum in hydroponically cultivated plant samples (Sinapis alba L and Lolium perenne) is presented. A microwave digestion with HNO3, HClO4 and HCl acids with additional UV irradiation is followed by the platinum determination by adsorptive stripping voltammetry at hanging mercury drop electrode. The influence of HNO3 acid on voltammetric determination of platinum is discussed. For validation of the obtained results the recovery of Pt was examined and the Pt determination by ICP MS was carried out. The highest platinum amounts were found in the roots of the plants, nevertheless the effective transport of platinum to the steams and leaves was also observed.
Electrocatalytic Reduction of Oxygen at Multi‐Walled Carbon Nanotubes and Cobalt Porphyrin Modified Glassy Carbon Electrode
Authors: Qu Jianying , Shen Yan , Qu Xiaohu , Dong Shaojun
Published: June 2004

The multi‐walled carbon nanotubes (MWNTs) modified glassy carbon electrode exhibited electrocatalytic activity to the reduction of oxygen in 0.1 M HAc‐NaAc (pH 3.8) buffer solution. Further modification with cobalt porphyrin film on the MWNTs by adsorption, the resulted modified electrode showed more efficient catalytic activity to O2 reduction. The reduction peak potential of O2 is shifted much more positively to 0.12 V (vs. Ag/AgCl), and the peak current is increased greatly. Cyclic voltammetry (CV), transmission electron microscopy (TEM), X‐ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM), were used to characterize the material and the modified film on electrode surface. Electrochemical experiments gave the total number of electron transfer for oxygen reduction as about 3, which indicated a co‐exist process of 2 electrons and 4 electrons for reduction of oxygen at this modified electrode. Meanwhile, the catalytic activities of the multilayer film (MWNTs/CoTMPyP)n prepared by layer‐by‐layer method were investigated, and the results showed that the peak current of O2 reduction increased and the peak potential shifted to a positive direction with the increase of layer numbers.
Monitoring of drugs and metabolites in body fluids by capillary electrophoresis with XeHg lamp‐based and laser‐induced fluorescence detection
Authors: Caslavska Jitka , Thormann Wolfgang
Published: June 2004

Commercial capillary electrophoresis instrumentation with XeHg lamp‐based and laser induced fluorescence (LIF) detection is employed for analysis of urinary 3,4‐methylenedioxymethamphetamine (MDMA, Ecstasy) and its major metabolites, urinary metabolites of acetylsalicylic acid, urinary benzoylecgonine in an immunoassay format, and albendazole sulfoxide and albendazole sulfone in plasma. For the examples studied, the data suggest that the lamp‐based detector can be employed for the monitoring of pharmacological and toxicological relevant solute concentrations, and thus represents an attractive alternative to LIF detection.
High‐density lipoproteins contribute to the α‐1‐globulin zone in capillary electrophoresis of human serum proteins
Authors: Luraschi Paola , Pagani Anna , Infusino Ilenia , Moraschinelli Irene , Franzini Carlo
Published: June 2004

α‐1‐Antitrypsin (AAT) and α‐1‐acid glycoprotein (AAG) are reported to be the main proteins contributing to the α‐1‐globulin capillary zone electrophoresis (CZE) zone, but the sum (AAT + AAG) showed lower than the α‐1‐globulin. We investigated the role of high‐density lipoprotein (HDL), an additional protein migrating in the α‐1‐globulin zone, as a possible cause for such a gap. In a set of 98 sera we measured the α‐1‐globulin with a dedicated clinical capillary electrophoresis system, and AAT, AAG and apolipoprotein A‐1 (ApoA) by immunonephelometry. The α‐1‐globulin were consistently higher than the sum (AAT + AAG), by (mean value ± standard deviation) 1.70 ± 0.88 g/L in 49 sera with low ApoA, and by 3.59 ± 0.75 g/L in 49 sera with high ApoA. Corresponding figures in the comparison α‐1‐globulin/(AAT + AAG + ApoA) were reduced to 1.08 ± 0.77 g/L and 1.67 ± 0.70 g/L. It is concluded that HDL significantly contribute to the CZE α‐1‐globulin zone, but do not completely explain the differences between the electrophoretic and the immunochemical measurements. However, CZE α‐1‐globulin measurements give information about increases of the two major acute phase proteins comparable to specific protein measurements.
Fenofibrate and fenofibric acid analysis by capillary electrophoresis
Authors: Shihabi Zak K.
Published: June 2004

A capillary electrophoresis method has been developed to measure fenofibrate in capsules based on micellar electrokinetic capillary chromatography with detection at 280 nm using a borate buffer containing sodium dodecyl sulfate (SDS). However, the metabolite of this drug (fenofibric acid) in serum and whole blood was analyzed by capillary zone electrophoresis (CZE) in a borate‐carbonate buffer using acetonitrile stacking. The analysis is rapid, < 7 min with no interferences. Incubation of fenofibrate in whole blood caused hydrolysis of the ester bond with the release of fenofibric acid.
Conflicting results between electrophoresis methods of serum M‐proteins
Authors: Wuyts Birgitte , Bossuyt Xavier , Verhoef Gregor , Blanckaert Norbert , Delanghe Joris R.
Published: June 2004

The quantification of monoclonal immunoglobulins by protein electrophoresis has been helpful in determining the prognosis for the patient. In a 65‐year old man with documented IgG λ monoclonal gammopathy, a discrepancy in the detection and quantification of this M‐protein was found when studied by four different electrophoretic methods. On a high‐resolution agarose method with acid violet staining a prominent peak was seen in the fast γ‐region. A lower resolution agarose method using an amido black staining showed a small mid‐γ restriction, and no spike was detected by high‐resolution or lower resolution capillary zone electrophoresis. The discrepancy was not attributable to a migration in the β‐region leading to masking by transferrin or C3 peak, incorrect separation on CZE due to a high isoelectric point (pI > 8.5) or pentamerization of IgM M‐proteins. Therefore, other causes for discrepancies should be investigated.
Determination of aminothiols in body fluids, cells, and tissues by capillary electrophoresis
Authors: Bayle Christophe , Caussé Elisabeth , Couderc François
Published: June 2004

Oxidative stress is present in cardiovascular diseases and hyperhomocysteinemia, an independant risk factor for these diseases. It may play a role by inducing production of oxygen free radicals. Reduced glutathione is the most abundant intracellular low‐molecular‐weight thiol and plays an essential role in protecting cells from toxic species. The thiol‐containing compounds which are the most often considered in biological analysis, are homocysteine (Hcy), cysteine (Cys), glutathione (GSH), cysteinyl‐glycine (Cys‐Gly), γ‐glutamyl‐cysteine (γGlu‐Cys), and their derivatives. These aminothiols are present in body fluids or cells, associated with proteins or occur free (reduced and oxidized). These free forms may play a role in the pathogenesis of disease. Because Hcy (with Cys) exhibits pro‐oxidative properties and GSH (with Cys‐Gly) antioxidative properties, and because there is extensive interconversion between these metabolites, their simultaneous analysis in biological samples is necessary to examine their role in human disease. Capillary electrophoresis (CE) seems to be a solution to reach this goal. No extensive review reports the analysis of aminothiols using CE. This review describes the different CE approaches which have been used to separate and assay aminothiols, and the different obtained datas.
Analysis of organic components of smokeless gunpowders: High‐performance liquid chromatogaphyvs.micellar electrokinetic capillary chromatography
Authors: Cascio Orazio , Trettene Maristella , Bortolotti Federica , Milana Gabriella , Tagliaro Franco
Published: June 2004

The aim of the present study was to verify the analytical performances of high‐performance liquid chromatography (HPLC) and micellar electrokinetic capillary chromatography (MEKC) for the separation and qualitative determination of a selected group of organic components of smokeless gunpowders. The HPLC method was based on a gradient reversed‐phase elution with a mobile phase composed of 0.17 MH3PO4/methanol; detection was performed by UV absorption at the wavelengths of 220, 254, and 270 nm. The MEKC experiments were carried out by using uncoated fused‐silica capillaries (50 μm inside diameter, 50 cm effective length) and a running buffer composed of 10 mMsodium tetraborate at pH 9.24 added with 25 mMsodium dodecyl sulfate (SDS); the applied voltage was 25 kV; detection was either at a fixed wavelength UV of 214 nm or with a diode‐array detector operating in the wavelength range from 190 to 350 nm. Both reversed‐phase HPLC and MEKC techniques succeeded in resolving the tested standard mixtures of organic components of smokeless powders. Although the sequence of elution of the different analytes was slightly different between HPLC and MEKC, a statistical analysis based on the Spearman's rank correlation test showed that the two separation patterns were highly correlated. HPLC and MEKC were comparable in terms of elution/migration time precision, whereas MEKC showed higher reproducibility of peak areas. The interfacing of capillary electrophoresis with diode array UV detection provided distinct UV spectra of the individual analytes, thus improving, on the detection side, the analytical selectivity and identification power of capillary electrophoresis.
Stacking and low‐temperature technique in nonaqueous capillary electrophoresis for the analysis of 3,4‐methylenedioxymethamphetamine
Authors: Tsai Chih‐Hsin , Fang Ching , Liu Ju‐Tsung , Lin Cheng‐Huang
Published: June 2004

Low‐temperature and ambient‐temperature nonaqueous stacking techniques in capillary electrophoresis (CE) are described for the first time. A low‐temperature bath was used to control the temperature from ambient to subzero degrees, by which a novel hyphenated method, low‐temperature bath‐nonaqueous capillary electrophoresis stacking (LTB‐NACE stacking) is demonstrated. 3,4‐Methylenedioxymethamphetamine (3,4‐MDMA) was determined at a concentration of 4.7×10−6 M (at a 92.1% confidence level) by normal nonaqueous capillary zone electrophoresis (NACZE) and this was improved to 2.6×10−8 M and 5.0×10−9 M, respectively, when the NACZE stacking and LTB‐NACZE stacking techniques were applied. The content of 3,4‐MDMA in an illicit drug and a suspect urine sample was readily detected. Upon application of the LTB to the separation of isomers the resolution (R) for the separation of 2,3‐/3,4‐MDMA was improved from 0.6 (LTB, 22°C) to 1.6 (LTB, −55°C) and for (+)3,4‐MDMA/(−)3,4‐MDMA, from 0.4 (LTB, 25°C) to 1.0 (LTB, −10°C).
Capillary electrophoresis and mass spectrometry for screening of metabolic disorders in newborns
Authors: Šenk Petr , Kozák Libor , Foret František
Published: June 2004

Clinical analyses always represent a challenge for the sensitivity and selectivity of the analytical techniques. Of the most critical are the techniques required for the quick determination of the disease state and application of the proper treatment in newborns. This short critical review overviews the present state of the art of the use of mass spectrometry and capillary electrophoresis for screening of metabolic disorders in newborns.
Determination of salbutamol enantiomers in human urine using heptakis(2,3‐di‐O‐acetyl‐6‐O‐sulfo)‐β‐cyclodextrin in nonaqueous capillary electrophoresis
Authors: Servais Anne‐Catherine , Chiap Patrice , Hubert Philippe , Crommen Jacques , Fillet Marianne
Published: June 2004

Nonaqueous capillary electrophoresis (NACE) was successfully applied to the resolution and the determination of salbutamol enantiomers in urine samples using heptakis(2,3‐di‐O‐acetyl‐6‐O‐sulfo)‐β‐cyclodextrin (HDAS‐β‐CD). After optimization of the electrophoretic parameters, namely the background electrolyte (BGE) composition and the HDAS‐β‐CD concentration, salbutamol enantiomers were completely resolved using a BGE made up of 10 mM ammonium formate and 15 mM HDAS‐β‐CD in methanol acidified with 0.75 M formic acid. Isoprenaline was selected as internal standard. Solid‐phase extraction (SPE) was used for sample cleanup prior to the CE separation. Different sorbents involving polar, nonpolar interactions or dual retention mechanisms were evaluated and extraction cartridges containing both nonpolar and strong cation‐exchange functionalities were finally selected. Salbutamol enantiomers recoveries from urine samples were determined. The method was then successfully validated using a new approach based on accuracy profiles over a concentration range from 375 to 7500 ng/mL for each enantiomer.
Capillary and microelectrophoretic separations of ligase detection reaction products produced from low‐abundant point mutations in genomic DNA
Authors: Thomas Gloria , Sinville Rondedrick , Sutton Shelby , Farquar Hannah , Hammer Robert P. , Soper Steven A. , Cheng Yu‐Wei , Barany Francis
Published: June 2004

Capillary gel electrophoresis (CGE) and polymer‐based microelectrophoretic platforms were investigated to analyze low‐abundant point mutations in certain gene fragments with high diagnostic value for colorectal cancers. The electrophoretic separations were carried out on single‐stranded DNA (ssDNA) products generated from an allele‐specific ligation assay (ligase detection reaction, LDR), which was used to screen for a single base mutation at codon 12 in the K‐ras oncogene. The presence of the mutation generated a ssDNA fragment that was >40 base pairs (bp) in length, while the primers used for the ligation assay were <30 bp in length. Various separation matrices were investigated, with the success of the matrix assessed by its ability to resolve the ligation product from the large molar excess of unligated primers when the mutant allele was lower in copy number compared to the wild‐type allele. Using CGE, LDR product models (44 and 51 bp) could be analyzed in a cross‐linked polyacrylamide gel with a 1000‐fold molar excess of LDR primers (25 bp) in ∼45 min. However, when using linear polyacrylamide gels, these same fragments could not be detected due to significant electrokinetic biasing during injection. A poly(methylmethacrylate) (PMMA) microchip of 3.5 cm effective column length was used with a 4% linear polyacrylamide gel to analyze the products generated from an LDR. When the reaction contained a 100‐fold molar excess of wild‐type DNA compared to a G12.2D mutant allele, the 44 bp ligation product could be effectively resolved from unligated primers in under 120 s, nearly 17 times faster than the CGE format. In addition, sample cleanup was simplified using the microchip format by not requiring desalting of the LDR prior to loading.