bluuebunny/crossref_metadata_2025 huggingface dataset:
Fibroblast Growth Factor 21 Ameliorates NaV1.5 and Kir2.1 Channel Dysregulation in Human AC16 Cardiomyocytes
Authors: Li Jiamin , Li Yuanshi , Liu Yining , Yu Hang , Xu Ning , Huang Di , Xue Yadong , Li Sijia , Chen Haixin , Liu Jiali , Li Qingsui , Zhao Yiming , Zhang Ronghao , Xue Hongru , Sun Yuehang , Li Ming , Li Pengyu , Liu Mingbin , Zhang Zhen , Li Xin , Du Weijie , Wang Ning , Yang Baofeng
Published: September 2021

Infarcted myocardium is predisposed to cause lethal ventricular arrhythmias that remain the main cause of death in patients suffering myocardial ischemia. Liver-derived fibroblast growth factor 21 (FGF21) is an endocrine regulator, which exerts metabolic actions by favoring glucose and lipids metabolism. Emerging evidence has shown a beneficial effect of FGF21 on cardiovascular diseases, but the role of FGF21 on ventricular arrhythmias following myocardial infarction (MI) in humans has never been addressed. This study was conducted to investigate the pharmacological effects of FGF21 on cardiomyocytes after MI in humans. Patients with arrhythmia in acute MI and healthy volunteers were enrolled in this study. Serum samples were collected from these subjects on day 1 and days 7–10 after the onset of MI for measuring FGF21 levels using ELISA. Here, we found that the serum level of FGF21 was significantly increased on day 1 after the onset of MI and it returned to normal on days 7–10, relative to the Control samples. In order to clarify the regulation of FGF21 on arrhythmia, two kinds of arrhythmia animal models were established in this study, including ischemic arrhythmia model (MI rat model) and nonischemic arrhythmia model (ouabain-induced guinea pig arrhythmia model). The results showed that the incidence and duration time of ischemic arrhythmias in rhbFGF21-treated MI rats were significantly reduced at different time point after MI compared with normal saline-treated MI rats. Moreover, the onset of the first ventricular arrhythmias was delayed and the numbers of VF and maintenance were attenuated by FGF21 compared to the rhbFGF21-untreated group in the ouabain model. Consistently, in vitro study also demonstrated that FGF21 administration was able to shorten action potential duration (APD) in hydrogen peroxide-treated AC16 cells. Mechanically, FGF21 can ameliorate the electrophysiological function of AC16 cells, which is characterized by rescuing the expression and dysfunction of cardiac sodium current (INa) and inward rectifier potassium (Ik1) in AC16 cells induced by hydrogen peroxide. Moreover, the restorative effect of FGF21 on NaV1.5 and Kir2.1 was eliminated when FGF receptors were inhibited. Collectively, FGF21 has the potential role of ameliorating transmembrane ion channels remodeling through the NaV1.5/Kir2.1 pathway by FGF receptors and thus reducing life-threatening postinfarcted arrhythmias, which provides new strategies for antiarrhythmic therapy in clinics.
Tuo-Min-Ding-Chuan Decoction Alleviate Ovalbumin-Induced Allergic Asthma by Inhibiting Mast Cell Degranulation and Down-Regulating the Differential Expression Proteins
Authors: Qin Jingbo , Lv Mingsheng , Jiang Zeqiang , Meng Xianghe , Wang Yi , Cui Jiarui , Wang Ji , Wang Qi
Published: September 2021

Allergic asthma is a stubborn chronic inflammatory disease, and is considered a co-result of various immune cells, especially mast cells, eosinophils and T lymphocytes. At present, the treatment methods of allergic asthma are limited and the side effects are obvious. Traditional Chinese medicine has been used to treat diseases for thousands of years in China. One such example is the treatment of allergic asthma, which take the characteristics of less adverse reactions and obvious curative effect. Tuo-Min-Ding-Chuan Decoction (TMDCD) is a traditional Chinese medicine compound for the treatment of allergic asthma optimized from Ma-Xing-Gan-Shi Decoction (MXGSD), which was put forward in Treatise on Febrile Diseases by Zhang Zhongjing in the Eastern Han Dynasty. The compound shows a significant clinical effect, but the mechanism of its influence on the immune system is still unclear. The purpose of this study was to observe whether TMDCD could alleviate the symptoms of ovalbumin (OVA) challenged allergic asthma mice, and to explore its immune regulatory mechanism, especially on mast cell (MC) degranulation. The results showed TMDCD could not only reduce the airway hyperresponsiveness (AHR), inflammatory cell infiltration and mucus secretion in the lung tissue of OVA challenged mice, but also decrease the levels of total IgE, OVA-specific IgE, histamine and LTC4 in serum. We found that TMDCD can downregulate the expression of Fractalkine, Tryptase ε, IL-25, CCL19, MCP-1, OX40L, Axl, CCL22, CD30, G-CSF, E-selectin, OPN, CCL5, P-selectin, Gas6, TSLP in OVA challenged mice serum by using mouse cytokines antibody array. It has been reported in some literatures that these differentially expressed proteins are related to the occurrence of allergic asthma, such as tryptase ε, MCP-1, CCL5, etc. can be released by MC. And the results of in vitro experiments showed that TMDCD inhibited the degranulation of RBL-2H3 cells stimulated by DNP-IgE/BSA. Taken together, we made the conclusion that TMDCD could reduce the infiltration of inflammatory cells in lung tissue and alleviate airway remodeling in mice with allergic asthma, showed the effects of anti-inflammatory and antiasthmatic. TMDCD could also reduce the levels of IgE, histamine, LTC4, Tryptase ε, and other MC related proteins in the serum of allergic asthma mice, and the in vitro experiments showed that TMDCD could inhibit IgE mediated degranulation and histamine release of RBL-2H3 cells, proved its anti allergic effect.
Light Localization and Principal Mode Propagation in Optical Fibers
Authors: Nolan Daniel A. , Nguyen Dan T.
Published: September 2021

The capacity of optical fiber communications has grown exponentially since its implementation decades ago. Optical fiber amplifiers, wavelength division multiplexing, and coherent communications have all enabled discontinuous growth. Space division multiplexing is proposed as the next discontinuity. Here tens of modes rather than a single mode are utilized in the transmission. Random scattering due to index fluctuations within the optical fiber cause coupling among the modal channels thereby degrading signal transmission. Principal mode transmission overcomes this limitation. Here a set of modes arrive localized at the fiber output unscattered. We review this methodology as it relates to optical communication capacity, but also as it relates to light localization. We also review the characterization of these modes both theoretically and experimentally.
Effect of Training and Detraining in the Components of Physical Fitness in People Living With HIV/AIDS
Authors: Araujo Juliany de Souza , Medeiros Rafaela Catherine da Silva Cunha de , Silva Tatiane Andreza Lima da , Medeiros Danielle Coutinho de , Medeiros Jason Azevedo de , Santos Isis Kelly dos , Wilde Phelipe , Cabral Breno Guilherme de Araújo Tinoco , Medeiros Radamés Maciel Vitor , Dantas Paulo Moreira Silva
Published: September 2021

The aim of the study was to evaluate the effect of training and detraining on the physical fitness components of people living with HIV/AIDS (PLHA). The study was characterized as experimental with a sample composed of 21 people divided into two groups: 11 volunteers (PLHA, 46.9 ± 8.0 years, 63.8 ± 12.7 kg, 161.7 ± 8.7 cm, 7 men, and 4 women), using antiretroviral therapy (ART) and 10 people without HIV/AIDS in the control group (CG, 43.8 ± 13.8 years, 75.2 ± 11.2 kg, 163.3 ± 7.8 cm, 3 men, and 7 women), with the same average age and level of physical activity. The intervention, applied to both groups, consisted of combined training for 15 weeks, followed by detraining for 5 weeks. Before and after the training and detraining period the following parameters were evaluated: body composition by dual energy radiological absorptiometry (DXA), cardiorespiratory fitness by ergospirometer, and strength of upper and lower limbs by isometric dynamometer. The results show the effect of the intervention moments on the strength and oxygen consumption variables (time factor), considering the two study groups. Regarding the analysis of the interaction (group vs. time), there was a significant effect on the isometric extension strength of the left (p = 0.019) and right (p = 0.030) knees, with training (left: 10.4%; right: 12.4%) and detraining (left: −10.8%; right: −12.1%) effect in PLHA, when compared with the control group (left: 8.1 and 3.9%, respectively; right: 11.5 and −0.2%, respectively). In addition, there was a significant interaction on ventilatory threshold 1 (p = 0.002), indicating a significantly greater increase with training (27.3%) and decrease with detraining (−22.7%) in the PLHA group compared with the Control group (19.9 and −6.7%, respectively). In conclusion, combined training and the subsequent period of detraining caused similar responses in body composition, isometric strength, and cardiorespiratory fitness of PLHA and CG, except for the extensor strength of the lower limbs and ventilatory threshold 1, which presented positive effects on training and negative effects on detraining for PLHA.

Clinical Trial Registration:www.ClinicalTrials.gov, identifier NCT03075332.
Electrocardiographic Scaling Reveals Differences in Electrocardiogram Interval Durations Between Marine and Terrestrial Mammals
Authors: Storlund Rhea L. , Rosen David A. S. , Trites Andrew W.
Published: September 2021

Although the ability of marine mammals to lower heart rates for extended periods when diving is well documented, it is unclear whether marine mammals have electrophysiological adaptations that extend beyond overall bradycardia. We analyzed electrocardiographic data from 50 species of terrestrial mammals and 19 species of marine mammals to determine whether the electrical activity of the heart differs between these two groups of mammals. We also tested whether physiological state (i.e., anesthetized or conscious) affects electrocardiogram (ECG) parameters. Analyses of ECG waveform morphology (heart rate, P-wave duration, and PQ, PR, QRS, and QT intervals) revealed allometric relationships between body mass and all ECG intervals (as well as heart rate) for both groups of mammals and specific differences in ECG parameters between marine mammals and their terrestrial counterparts. Model outputs indicated that marine mammals had 19% longer P-waves, 24% longer QRS intervals, and 21% shorter QT intervals. In other words, marine mammals had slower atrial and ventricular depolarization, and faster ventricular repolarization than terrestrial mammals. Heart rates and PR intervals were not significantly different between marine and terrestrial mammals, and physiological state did not significantly affect any ECG parameter. On average, ECG interval durations of marine and terrestrial mammals scaled with body mass to the power of 0.21 (range: 0.19–0.23) rather than the expected 0.25—while heart rate scaled with body mass to the power of –0.22 and was greater than the widely accepted –0.25 derived from fractal geometry. Our findings show clear differences between the hearts of terrestrial and marine mammals in terms of cardiac timing that extend beyond diving bradycardia. They also highlight the importance of considering special adaptations (such as breath-hold diving) when analyzing allometric relationships.
Mitochondrial Stress Induces Plant Resistance Through Chromatin Changes
Authors: López Sánchez Ana , Hernández Luelmo Sofía , Izquierdo Yovanny , López Bran , Cascón Tomás , Castresana Carmen
Published: September 2021

Plants respond more efficiently when confronted with previous similar stress. In the case of pathogens, this memory of a previous infection confers resistance to future ones, which possesses a high potential for agricultural purposes. Some of the defense elements involved in this resistance phenotype, as well as epigenetic mechanisms participating in the maintenance of the memory, are currently known. However, the intracellular cascade from pathogen perception until the establishment of the epigenetic memory is still unexplored. Here, through the induction of mitochondrial stress by exogenous applications of Antimycin A in Arabidopsis thaliana plants, we discovered and characterized a role of mitochondrial stress in plant-induced resistance. Mitochondrial stress-induced resistance (MS-IR) is effective locally, systemically, within generation and transgenerationally. Mechanistically, MS-IR seems to be mediated by priming of defense gene transcription caused by epigenetic changes. On one hand, we observed an increment in the deposition of H3K4me3 (a positive epigenetic mark) at the promoter region of the primed genes, and, on the other hand, the DNA (de)methylation machinery seems to be required for the transmission of MS-IR to the following generations. Finally, we observed that MS-IR is broad spectrum, restricting the colonization by pathogens from different kingdoms and lifestyles. Altogether, this evidence positions mitochondria as a prominent organelle in environment sensing, acting as an integrating platform to process external and internal signals, triggering the appropriate response, and inducing the epigenetic memory of the stress to better react against future stressful conditions.
4-Ethylphenol, A Volatile Organic Compound Produced by Disease-Resistant Soybean, Is a Potential Botanical Agrochemical Against Oomycetes
Authors: Ge Ting , Gao Wenteng , Liang Changhui , Han Chao , Wang Yong , Xu Qian , Wang Qunqing
Published: September 2021

Oomycetes, represented by Phytophthora, are seriously harmful to agricultural production, resulting in a decline in grain quality and agricultural products and causing great economic losses. Integrated management of oomycete diseases is becoming more challenging, and plant derivatives represent effective alternatives to synthetic chemicals as novel crop protection solutions. Biologically active secondary metabolites are rapidly synthesized and released by plants in response to biotic stress caused by herbivores or insects, as well as pathogens. In this study, we identified groups of volatile organic compounds (VOCs) from soybean plants inoculated with Phytophthora sojae, the causal agent of soybean root rot. 4-Ethylphenol was present among the identified VOCs and was induced in the incompatible interaction between the plants and the pathogen. 4-Ethylphenol inhibited the growth of P. sojae and Phytophthora nicotianae and had toxicity to sporangia formation and zoospore germination by destroying the pathogen cell membrane; it had a good control effect on soybean root rot and tobacco black shank in the safe concentration range. Furthermore, 4-Ethylphenol had a potent antifungal activity against three soil-borne phytopathogenic fungi, Rhizoctonia solani, Fusarium graminearum, and Gaeumannomyces graminis var tritici, and four forma specialis of Fusarium oxysporum, which suggest a potential to be an eco-friendly biological control agent.
Who Can Buffer Marginalization Risk? Affect Experience, Affect Valuation, and Social Marginalization in Japan and Brazil
Authors: De Almeida Igor , Uchida Yukiko
Published: September 2021

Previous research has associated social marginalization with the rejection of mainstream cultural values. Since cultural values reflect affect valuation, the present research investigates the relationships between social marginalization and ideal/actual affect in two different non-WEIRD cultures, Brazil and Japan. As a social marginalization index, we used the NEET-Hikikomori Risk Scale (NHR). We predicted that cultural differences would emerge in the valuation of affective states. Affect valuation theory suggests that in East Asia, individuals are encouraged to pursue and value low arousal positive emotions (LAP: e.g., calmness, serenity) over high arousal positive emotions (HAP: e.g., excitement, elation, etc.) as they can harm social relationships in these societies. In contrast, Latin American cultures value HAP over LAP, because social relationships are promoted through vibrant positive emotional expression in these cultures. Hence, we hypothesized that individuals’ ideal affect, actual affect, and the discrepancy between ideal and actual affect would be associated with higher risk of social marginalization. Participants from Japan (N = 54) and Brazil (N = 54) reported their ideal affect and actual affect and completed the NEET-Hikikomori Risk Scale (NHR). Regression analyses showed that actual HAP and the discrepancy between ideal and actual HAP were negatively associated with NHR in Brazil, but no association was found in the Japanese data. The other variables, including ideal affect, were only minorly or not significantly associated with NHR. Though the study has limitations regarding its small sample size, we can explore future perspectives and discuss the relationships between emotion and cultural marginalization. Socioecological factors that promote actual HAP in Brazilians may encourage other mainstream cultural ideals, which buffers against cultural marginalization.
Organizational Challenges of Development and Implementation of Virtual Reality Solution for Industrial Operation
Authors: Saghafian Mina , Laumann Karin , Skogstad Martin Rasmussen
Published: September 2021

This research investigated the organizational challenges related to the development and implementation of virtual reality (VR) technology for operation in a conservative heavy machinery industry. The incorporation of a VR solution for heavy machinery equipment enhanced the safety and convenience of operation under dangerous work conditions. However, the development and implementation processes faced challenges. Furthermore, the adoption of the solution by users was perceived to be slower than anticipated. We aimed to explore the main challenges that the developer organization faced and how it also influenced user organizations. Due to the exploratory nature of the research, qualitative analysis was chosen, interviews were conducted, and thematic analysis was applied. The themes and subthemes were identified and discussed. The results showed the existence of challenges related to technology maturity, managerial challenges regarding communication and support coordination, workload, and multiple stakeholder management. The findings emphasize the importance of attending to the existing and potential organizational challenges before and throughout technological innovation. Theoretical and managerial implications are discussed, and a future research agenda is suggested.
Mattering as a Political, Scientific, and Professional Basis for Welfare Services
Authors: Krokstad Steinar
Published: September 2021

The dominant political ideology of recent decades, neoliberalism, have resulted in diminished sense of mattering for several groups in the society, not at least people outside the labor market. This has left its mark on vocational rehabilitation programs in welfare states like Norway. Higher requirements shall be set for benefit recipients, and compulsory work are more often applied. The problem with this policy is that it suggests that benefit recipients have a guilt to make up for and are themselves to blame for the unemployment. However, the majority of people in need for vocational rehabilitation, have had poor living conditions since childhood, and have failed in education and employment for or reasons they have no control over. They often do not feel valued and have a lot of experience with not being able to add value. The problem with blaming the victims, is that it reinforces their sense of worthlessness, and thus reduces their ability to believe that they can contribute with something of value. In this way, the policy becomes counterproductive. Some even respond to these humiliating pressures by becoming more depressive or aggressive. To make vocational rehabilitation programs effective, we must make sure that everyone in need for it feel valued, we must align the political, scientific, and professional basis for welfare service politics thereafter. We must balance adding value to self with the opportunity to adding value to others, work and community. Mattering is suggested as a political, scientific, and professional basis for welfare services.
When Different Language Groups Meet Online: Covert and Overt Focus on Form in Text-Based Chats
Authors: Feng Ruiling , Pyun Kyunghee , Zhang Wenzhong , Márquez Flores Rafael
Published: September 2021

Focus on form has been extensively studied in text-based online dyadic chats but much less has been explored in group chats with interlocutors from different language backgrounds. Additionally, there are very few studies investigating covert focus on form. This study investigated the effects of interlocutor types on errors and focus on form episodes, both covert and overt, in text-based online group chats. We collected chat logs from two collaborative online international learning projects. One project was developed for the collaboration between an English course at a Chinese university and an art history course at a U.S. university; the other between another cohort of the same English course and a cultural studies course at a Mexican university. We compared errors, feedback, and other characteristics of focus on form episodes between the two projects. Analyses revealed significant differences in characteristics such as overtness (overt, covert), linguistic focus (mechanical, lexical, and grammatical), and source (code, message). However, no significant differences were found for the type of focus on form (preemptive, reactive), presence of uptake, uptake quality (successful, unsuccessful), and repair provider (self, other). Students showed a preference for self-repair over other-repair and for lexical focus over mechanical and grammatical foci in both projects. Overall, only a small proportion of errors were followed by feedback. Therefore, a small amount of uptake and successful uptake occurred in both projects. The results can shed light on how instructors could provide effective scaffolding and tasks to make virtual exchange projects more rewarding.
What Drives Outpatient Care Costs in Kenya? An Analysis With Generalized Estimating Equations
Authors: Mwenda Ngugi , Nduati Ruth , Kosgei Mathew , Kerich Gregory
Published: September 2021

Objective: This study aimed to identify the factors associated with outpatient expenses incurred by households in Kenya.

Background: The problem of outpatient healthcare expenses incurred by citizens in countries with limited resources has received little attention. Thus, this study aimed to determine the predictors of household spending on outpatient expenses in Kenya.

Method: We conducted a cross-sectional analysis on households in Kenya using data from the 2018 Kenya Household Health Expenditure and Utilization Survey. We applied the generalized estimating equations method to determine the best subset of predictors of outpatient care cost.

Findings: The best predictors of outpatient care expenses in Kenya are age, wealth index, and education level of the household head.

Conclusions: There were no differences regarding age in the mean spending on outpatient care. Moreover, we found that the cost of outpatient care changes with age in a sinusoidal manner. We observed that rich households spent more on outpatient care, mostly owing to their financial ability. Households whose heads reported primary or secondary school education level spent less on outpatient costs than households headed by those who never went to school.
5G New Radio Requires the Best Possible Risk Assessment Studies: Perspective and Recommended Guidelines
Authors: Mattsson Mats-Olof , Simkó Myrtill , Foster Kenneth R.
Published: September 2021

The development and establishment of mobile communication technologies has necessitated assessments of possible risks to human health from exposures to radio-frequency electromagnetic fields (RF EMF). A number of expert committees have concluded that there is no evidence for such risks as long as exposures are at or below levels that do not allow tissue heating. These assessments have been based primarily on studies investigating frequencies up to 6 GHz including frequencies similar to those used by two of three major bands of fifth generation (more accurately 5G New Radio or 5G NR) of mobile communication. Bioeffects studies in so-called high-band at 25–39 GHz are particularly sparse. Future assessments relevant for these frequencies will need to rely on still unperformed studies. Due to few available studies at 5G NR “high band” frequencies, and questions raised by some existing studies, a recent review recommended a wide range of RF biostudies be done at 5G NR “high band” frequencies. It is of importance that such studies be done using the best possible science. Here we suggest factors to consider when performing future studies in this area. The present focus is on laboratory studies to clarify biological effects of radiofrequency (RF) energy at 5G “high band” frequencies and, more generally at millimeter wave (mm-wave) frequencies (30-300 GHz) which will be increasingly used by communications technologies in the future. Similar comments would apply to epidemiology and exposure assessment studies, but those are not the focus of the present Perspective.
Proteome Modulation in Peripheral Blood Mononuclear Cells of Peste des Petits Ruminants Vaccinated Goats and Sheep
Authors: Wani Sajad Ahmad , Sahu Amit Ranjan , Khan Raja Ishaq Nabi , Praharaj Manas Ranjan , Saxena Shikha , Rajak Kaushal Kishor , Muthuchelvan Dhanavelu , Sahoo Aditya , Mishra Bina , Singh R. K. , Mishra Bishnu Prasad , Gandham Ravi Kumar
Published: September 2021

In the present study, healthy goats and sheep (n= 5) that were confirmed negative forpeste des petitsruminants virus (PPRV) antibodies by monoclonal antibody-based competitive ELISA and by serum neutralization test and for PPRV antigen by s-ELISA were vaccinated with Sungri/96. A quantitative study was carried out to compare the proteome of peripheral blood mononuclear cells (PBMCs) of vaccinated goat and sheep [5 days post-vaccination (dpv) and 14 dpv] vs. unvaccinated (0 day) to divulge the alteration in protein expression following vaccination. A total of 232 and 915 proteins were differentially expressed at 5 and 14 dpv, respectively, in goats. Similarly, 167 and 207 proteins were differentially expressed at 5 and 14 dpv, respectively, in sheep. Network generated by Ingenuity Pathway Analysis was “infectious diseases, antimicrobial response, and inflammatory response,” which includes the highest number of focus molecules. The bio functions, cell-mediated immune response, and humoral immune response were highly enriched in goats at 5 dpv and at 14 dpv. At the molecular level, the immune response produced by the PPRV vaccine virus in goats is effectively coordinated and stronger than that in sheep, though the vaccine provides protection from virulent virus challenge in both. The altered expression of certain PBMC proteins especially ISG15 and IRF7 induces marked changes in cellular signaling pathways to coordinate host immune responses.
Crop Diversity Management System Commons: Revisiting the Role of Genebanks in the Network of Crop Diversity Actors
Authors: Louafi Sélim , Thomas Mathieu , Berthet Elsa T. , Pélissier Flora , Vaing Killian , Jankowski Frédérique , Bazile Didier , Pham Jean-Louis , Leclercq Morgane
Published: September 2021

This paper rethinks the governance of genebanks in a social and political context that has significantly evolved since their establishment. The theoretical basis for the paper is the commons conceptual framework in relation to both seed and plant genetic resources. This framework is applied to question the current policy ecosystem of genetic research and breeding and explore different collective governance models. The concept of crop diversity management system (CDMS) commons is proposed as the new foundation for a more holistic and inclusive framework for crop diversity management, that covers a broad range of concerns and requires different actors. The paper presents a multi-stakeholder process established within the context of the two recent projects CoEx and Dynaversity, imagining possible collective arrangements to overcome existing deadlocks, foster collective learning, and design collaborative relationships among genebanks, researchers, and farmers’ civil society organizations involved in crop diversity management.
Crop Establishment Methods and Integrated Nutrient Management Improve: Part II. Nutrient Uptake and Use Efficiency and Soil Health in Rice (Oryza sativa L.) Field in the Lower Indo-Gangetic Plain, India
Authors: Midya Ashim , Saren Binoy Kumar , Dey Joy Kumar , Maitra Sagar , Praharaj Subhashisa , Gaikwad Dinkar Jagannath , Gaber Ahmed , Alhomrani Majid , Hossain Akbar
Published: September 2021

Rice, the predominant food crop in India, is being grown traditionally with improper plant nutrient management mostly under the flooded situation. Recent advancement in research on crop science focuses on water-saving rice technologies for maximization in crop and water productivity under the backdrop of a shrinking water resource base for ensuring environmental and agricultural sustainability. Under this situation, an experiment was conducted in two consecutive years in a split-plot design keeping rice cultivation methodologies, viz., aerobic culture, System of Rice Intensification (SRI), and conventional flooded culture in main plots and integrated plant nutrient management (INM) treatments in sub-plots. The experiment was aimed at understanding the effects of different rice production systems and INM on nutrient content, uptake, and use efficiency. The change in soil quality parameters was also studied to understand the impact of crop establishment methods (CEM) and INM options. Significant reduction (p ≤ 0.05) in nutrient uptake and use efficiency was observed under aerobic culture compared to SRI and flooded method, although aerobic culture showed the highest physiological nitrogen use efficiency. Post-harvest available Fe status was significantly lower in aerobic rice (mean 10.39 ppm) compared to other crop establishment technologies; however, Zn status was higher in aerobic rice over the flooded situation. Although available potassium was not affected due to rice cultivation methods, available nitrogen and phosphorus status were influenced remarkably. Soil microbial quality was improved in aerobic rice in comparison to flooded rice. SRI proved to be the most efficient rice establishment method for enhancement in nutrient uptake, use efficiency, and enrichment of soil chemical and microbiological quality. Irrespective of crop culture, integrated plant nutrition in rice improved the nutrient uptake, use efficiency, and soil quality parameters. The study revealed that, under the alluvial soils of the Indo-Gangetic Plains of Eastern India, SRI can be considered as a water-saving rice production method. The method can also improve nutrient uptake, efficiency, and soil quality parameters if proper INM is adopted.
Exogenous Glomalin-Related Soil Proteins Differentially Regulate Soil Properties in Trifoliate Orange
Authors: Liu Rui-Cheng , Zou Ying-Ning , Kuča Kamil , Hashem Abeer , Abd_Allah Elsayed Fathi , Wu Qiang-Sheng
Published: September 2021

Glomalin-related soil protein (GRSP) is a specific glycoprotein secreted into the soil by hyphae and spores of arbuscular mycorrhizal fungi that have many potential functions. It is not clear whether exogenous GRSP has an effect on plant growth and soil properties or whether the effects are related to the type of GRSP used. In this study, trifoliate orange (Poncirus trifoliata L. Raf.) seedlings were used to analyze the effects of easily extractable GRSP (EE-GRSP) and difficultly extractable GRSP (DE-GRSP) at a quarter-, half-, and full-strength concentration on shoot and root biomass as well as soil properties The results showed that, at different strengths, exogenous EE-GRSR significantly increased shoot and root biomass compared to the control, which displayed the most significant effects from the half-strength EE-GRSP. In contrast, DE-GRSP, at various strengths, significantly reduced shoot and root biomass. Furthermore, the application of exogenous EE-GRSP stimulated soil water-stable aggregate (WSA) content at 2–4 mm and 0.5–1 mm sizes, while DE-GRSP strongly reduced WSA content at the 2–4 mm, 1–2 mm, 0.5–1 mm, and 0.25–0.5 mm sizes, consequently leading to an increase or decrease in the WSA stability, according to the mean weight diameter. However, exogenous EE-GRSP decreased soil pH and DE-GRSP increased it, which was related to WSA stability. Exogenous EE-GRSP almost significantly increased soil acidic, neutral, and alkaline phosphatase activity at different strengths, while exogenous DE-GRSP, also at different strengths, significantly inhibited soil acidic phosphatase activity. The application of both exogenous EE-GRSP and DE-GRSP increased the organic carbon content of the soil. This study concluded that exogenous GRSP exerted differential effects on plant biomass and soil properties, and EE-GRSP can be considered as a soil stimulant for use in citrus plants. To our knowledge, this is the first report on the negative effects of exogenous DE-GRSP on plant biomass and soil properties.
Effect of Different Forms of Sulfur Fertilization on Bioactive Components and Antioxidant Activity of White Cabbage (Brassica Oleracea L.)
Authors: Nawirska-Olszańska Agnieszka , Biesiada Anita , Kita Agnieszka
Published: September 2021

Cruciferous vegetables are very popular in latitudes corresponding to central and eastern Europe. They are rich in bioactive compounds such as chlorophylls, carotenoids, and polyphenols. The type and quality of fertilization has a significant impact on their chemical composition. The aim of this study was to determine the impact of specific forms of sulfur fertilization on the chemical properties of white cabbage, and to explore the effect of storage conditions on its bioactive components and antioxidant activity. The research material was the late cultivar of white cabbage ‘Stonehead’, fertilized with a dose of 30 kg S·ha−1, administered in the form of elemental sulfur, ammonium sulfate, and potassium sulfate. Sulfur fertilization had a significant impact on the parameters selected for this study, and the form in which it was applied resulted in different effects on these individual parameters. For all parameters investigated, the effect of sulfur fertilization was detectable. Antioxidant properties, determined as both ABTS and DPPH radical-scavenging activities, were the highest in cabbage harvested from the plot treated with potassium sulfate
Acoustic Emission Spectroscopy: Applications in Geomaterials and Related Materials
Authors: Salje Ekhard K. H. , Jiang Xiang , Eckstein Jack , Wang Lei
Published: September 2021

As a non-destructive testing technology with fast response and high resolution, acoustic emission is widely used in material monitoring. The material deforms under stress and releases elastic waves. The wave signals are received by piezoelectric sensors and converted into electrical signals for rapid storage and analysis. Although the acoustic emission signal is not the original stress signal inside the material, the typical statistical distributions of acoustic emission energy and waiting time between signals are not affected by signal conversion. In this review, we first introduce acoustic emission technology and its main parameters. Then, the relationship between the exponents of power law distributed AE signals and material failure state is reviewed. The change of distribution exponent reflects the transition of the material’s internal failure from a random and uncorrelated state to an interrelated state, and this change can act as an early warning of material failure. The failure process of materials is often not a single mechanism, and the interaction of multiple mechanisms can be reflected in the probability density distribution of the AE energy. A large number of examples, including acoustic emission analysis of biocemented geological materials, hydroxyapatite (human teeth), sandstone creep, granite, and sugar lumps are introduced. Finally, some supplementary discussions are made on the applicability of Båth’s law.
High-Grade B-Cell Lymphoma Not Otherwise Specified (HGBL, NOS) in the Maxillary Sinus Mimicking Periapical Inflammation: Case Report and Review of the Literature
Authors: Brody Andrea , Dobo-Nagy Csaba , Mensch Karoly , Oltyan Zsuzsanna , Csomor Judit , Pacurar Mariana , Dobai Adrienn
Published: September 2021

High-grade B-cell lymphoma not otherwise specified is listed as a new group in the WHO 2017 statement as a subtype of aggressive, mature B-cell lymphomas with a poor prognosis. To our knowledge, no description of this genetic type of maxillary lymphoma has appeared in the literature until now; thus, our case provides valuable data on its symptoms, clinical behavior, response to treatment and survival rate. The present report describes the early diagnosis and treatment of an extremely rare histological subtype of B-cell lymphoma, a case of high-grade B-cell lymphoma not otherwise specified, localized in the maxillary sinus and mimicking signs and symptoms of periapical inflammation. After chemotherapy, the presented patient showed complete remission without relapse and systemic spread. As far as we know, this is the first reported case of this rare type of lymphoma associated with the maxillary sinus. Considering that high-grade B-cell lymphomas are aggressive tumors with rapid growth and poor prognosis, which are often misdiagnosed in the early stages as inflammatory disease, it is relevant to highlight the importance of a detailed evaluation of clinical signs and radiological findings during diagnosis, especially if they contradict each other.
Liver Transplantation for HCC in HIV-Infected Patients: Long-Term Single-Center Experience
Authors: Guerrini Gian Piero , Berretta Massimiliano , Guaraldi Giovanni , Magistri Paolo , Esposito Giuseppe , Ballarin Roberto , Serra Valentina , Di Sandro Stefano , Di Benedetto Fabrizio
Published: September 2021

Background: HIV-infected patients now have long life expectation since the introduction of the highly active antiretroviral therapy (HAART). Liver diseases, especially cirrhosis and hepatocellular carcinoma (HCC), currently represent a leading cause of death in this setting of patients. Aim: To address the results of liver transplantation (LT) for HCC in HIV-infected patients. Methods: All patients with and without HIV infection who underwent LT for HCC (n = 420) between 2001 and 2021 in our center were analyzed with the intent of comparing graft and patient survival. Cox regression analysis was used to determine prognostic survival factors and logistic regression to determine the predictor factors of post-LT recurrence. Results: Among 1010 LT, 32 were HIV-infected recipients. With an average follow-up of 62 ± 51 months, 5-year overall survival in LT recipients with and without HIV-infection was 71.6% and 69.9%, respectively (p = ns), whereas 5-year graft survival in HIV-infected and HIV-non infected was 68.3% and 68.2%, respectively (p = ns). The independent predictive factor of survival in the study group was: HCV infection (HR 1.83, p = 0.024). There were no significant differences in the pathological characteristics of HCC between the two groups. The logistic regression analysis of the study population demonstrated that microvascular invasion (HR 5.18, p< 0.001), HCC diameter (HR 1.16, p = 0.028), and number of HCC nodules (HR 1.26, p = 0.003) were predictors of recurrence post-LT. Conclusion: Our study shows that HIV patients undergoing LT for HCC have comparable results in terms of post-LT survival. Excellent results can be achieved for HIV-infected patients with HCC, as long as a strategy of close surveillance and precise treatment of the tumor is adopted while on the waiting list.
Regorafenib in Recurrent Glioblastoma Patients: A Large and Monocentric Real-Life Study
Authors: Lombardi Giuseppe , Caccese Mario , Padovan Marta , Cerretti Giulia , Pintacuda Giovanna , Manara Renzo , Di Sarra Francesca , Zagonel Vittorina
Published: September 2021

Despite multimodal treatment with surgery and radiochemotherapy, the prognosis of glioblastoma remains poor, and practically all glioblastomas relapse. To date, no standard treatment exists for recurrent glioblastoma patients and traditional therapies have showed limited efficacy. Regorafenib is an oral multi-targeted tyrosine kinase inhibitor showing encouraging benefits in recurrent GBM patients enrolled in the REGOMA trial. We performed a large study to investigate clinical outcomes and the safety of regorafenib in a real-life population of recurrent glioblastoma patients. Patients receiving regorafenib outside clinical trials at the Veneto Institute of Oncology were retrospectively reviewed. The major inclusion criteria were: histologically confirmed diagnosis of glioblastoma, prior first line therapy according to “Stupp protocol”, Eastern Cooperative Oncology Group (ECOG) performance status score ≤1. According to the original schedule, patients received regorafenib 160 mg once daily for the first 3 weeks of each 4-week cycle. The primary endpoints of the study were overall survival and safety. A total of 54 consecutive patients were enrolled. The median age was 56, MGMT methylated status was found in 28 out of 53 available patients (52.8%), IDH mutation in 5 (9.3%) and 22 patients were receiving steroids at baseline. The median overall survival was 10.2 months (95% CI, 6.4–13.9), the OS-12 was 43%. Age, MGMT methylation status and steroid use at baseline were not statistically significant on a multivariate analysis for OS. Patients reporting a disease control as best response to regorafenib demonstrated a significant longer survival (24.8 months vs. 6.2 months for patients with progressive disease, p = 0.0001). Grade 3 drug-related adverse events occurred in 10 patients (18%); 1 patient (2%) reported a grade 4 adverse event (rash maculo-papular). No death was considered to be drug-related. This study reported the first large “real-life” experience of regorafenib in recurrent glioblastoma. Overall, our results are close to the ones reported in the previous phase 2 study, despite the fact that we had a longer survival. We showed the encouraging activity and tolerability of this treatment in recurrent glioblastoma patients when used as a second-line treatment.
Decisional Regret in Female Oncofertility Decision Making—An Integrative Narrative Review
Authors: Gonçalves Vânia
Published: September 2021

It is well established that fertility is an important issue for young women with cancer at reproductive age, as many have not initiated or completed their parenthood goals when diagnosed. Because cancer treatments may impair fertility, women face fertility decisions that are often complex and surrounded by uncertainty. This may put patients at risk for psychological distress and the experience of regret regarding decisions made at diagnosis, which may be associated with a negative impact on women’s QoL. This narrative review addresses current knowledge about decisional regret regarding fertility preservation decisions in adult female cancer patients at reproductive age. Electronic searches were conducted on Pubmed database for articles published in English from 1 January 2000 to 1 July 2021 that assessed decisional regret following fertility decisions in young women diagnosed at childbearing age. Of the 96 articles identified, nine provided information on decisional regret regarding fertility decisions. Studies reported that, overall, decisional regret regarding oncofertility decisions was low. Factors associated with the experience of decisional regret were patients’ perceived quality and satisfaction with fertility counseling received, the decision to undergo fertility preservation, desire for children and decisional conflict. Health providers should be aware of the factors that are potentially modifiable and prone to improvement in order to reduce decisional regret. All efforts should be made to improve availability of and access to tailored high quality fertility counseling and fertility preservation. Given the growing evidence that decision aids (DAs) are effective in increasing knowledge and reducing decisional conflict and regret, their use in a routine and timely manner to complement fertility counseling is recommended.
Beyond the X Factor: Relevance of Sex Hormones in NAFLD Pathophysiology
Authors: Della Torre Sara
Published: September 2021

Non-alcoholic fatty liver disease (NAFLD) is a major health issue worldwide, being frequently associated with obesity, unbalanced dietary regimens, and reduced physical activity. Despite their greater adiposity and reduced physical activity, women show a lower risk of developing NAFLD in comparison to men, likely a consequence of a sex-specific regulation of liver metabolism. In the liver, sex differences in the uptake, synthesis, oxidation, deposition, and mobilization of lipids, as well as in the regulation of inflammation, are associated with differences in NAFLD prevalence and progression between men and women. Given the major role of sex hormones in driving hepatic sexual dimorphism, this review will focus on the role of sex hormones and their signaling in the regulation of hepatic metabolism and in the molecular mechanisms triggering NAFLD development and progression.
Characterization and In Vitro Studies of Low Reflective Magnetite (Fe3O4) Thin Film on Stainless Steel 420A Developed by Chemical Method
Authors: Aruvathottil Rajan Reghuraj , Saju Kaiprappady Kunchu , Aravindakshan Ritwik
Published: September 2021

Stainless steel has been the most demanded material for surgical utensil manufacture due to superior mechanical properties, sufficient wear, and corrosion resistance. Surgical grade 420A stainless steel is extensively used for producing sophisticated surgical instruments. Since these instruments are used under bright light conditions prevalent in operation theatres, the reflection from the material is significant which causes considerable strain to the eye of the surgeon. Surgical instruments with lower reflectance will be more efficient under these conditions. A low reflective thin -film coating has often been suggested to alleviate this inadmissible difficulty. This paper reports the development of an optimum parametric low reflective magnetite coating on the surface of SS 420A with a black color using chemical hot alkaline conversion coating technique and its bioactivity studies. Coating process parameters such as coating time, bath temperature, and chemical composition of bath are optimized using Taguchi optimization techniques. X-ray photoelectron spectroscopy (XPS) analysis was used to identify the composition of elements and the chemical condition of the developed coating. Surface morphological studies were accomplished with a scanning electron microscope (SEM). When coupled with an energy-dispersive X-ray analysis (EDAX), compositional information can also be collected simultaneously. Invitro cytotoxicity tests, corrosion behavior, the effect of sterilization temperature on adhesion property, and average percentage reflectance (R) of the developed coating have also been evaluated. These results suggest adopting the procedure for producing low reflective conversion coatings on minimally invasive surgical instruments produced from medical grade 420A stainless steel.