bluuebunny/crossref_metadata_2025 huggingface dataset:
Magnetic Hyperthermia Nanoarchitectonics via Iron Oxide Nanoparticles Stabilised by Oleic Acid: Anti-Tumour Efficiency and Safety Evaluation in Animals with Transplanted Carcinoma
Authors: Kulikov Oleg A. , Zharkov Mikhail N. , Ageev Valentin P. , Yakobson Denis E. , Shlyapkina Vasilisa I. , Zaborovskiy Andrey V. , Inchina Vera I. , Balykova Larisa A. , Tishin Alexander M. , Sukhorukov Gleb B. , Pyataev Nikolay A.
Published: April 2022

In this study, we developed iron oxide nanoparticles stabilised with oleic acid/sodium oleate that could exert therapeutic effects for curing tumours via magnetic hyperthermia. A suspension of iron oxide nanoparticles was produced and characterised. The toxicity of the synthesised composition was examined in vivo and found to be negligible. Histological examination showed a low local irritant effect and no effect on the morphology of the internal organs. The efficiency of magnetic hyperthermia for the treatment of transplanted Walker 256 carcinoma was evaluated. The tumour was infiltrated with the synthesised particles and then treated with an alternating magnetic field. The survival rate was 85% in the studied therapy group of seven animals, while in the control group (without treatment), all animals died. The physicochemical and pharmaceutical properties of the synthesised fluid and the therapeutic results, as seen in the in vivo experiments, provide insights into therapeutic hyperthermia using injected magnetite nanoparticles.
Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation
Authors: Hefka Blahnová Veronika , Vojtová Lucy , Pavliňáková Veronika , Muchová Johana , Filová Eva
Published: April 2022

Collagen I-based foams were modified with calcined or noncalcined hydroxyapatite or calcium phosphates with various particle sizes and pores to monitor their effect on cell interactions. The resulting scaffolds thus differed in grain size, changing from nanoscale to microscopic, and possessed diverse morphological characteristics and resorbability. The materials’ biological action was shown on human bone marrow MSCs. Scaffold morphology was identified by SEM. Using viability test, qPCR, and immunohistochemical staining, we evaluated the biological activity of all of the materials. This study revealed that the most suitable scaffold composition for osteogenesis induction is collagen I foam with calcined hydroxyapatite with a pore size of 360 ± 130 µm and mean particle size of 0.130 µm. The expression of osteogenic markers RunX2 and ColI mRNA was promoted, and a strong synthesis of extracellular protein osteocalcin was observed. ColI/calcined HAP scaffold showed significant osteogenic potential, and can be easily manipulated and tailored to the defect size, which gives it great potential for bone tissue engineering applications.
Bugs in Bugs: The Role of Probiotics and Prebiotics in Maintenance of Health in Mass-Reared Insects
Authors: Savio Carlotta , Mugo-Kamiri Loretta , Upfold Jennifer K.
Published: April 2022

Interactions between insects and their microbiota affect insect behaviour and evolution. When specific microorganisms are provided as a dietary supplement, insect reproduction, food conversion and growth are enhanced and health is improved in cases of nutritional deficiency or pathogen infection. The purpose of this review is to provide an overview of insect–microbiota interactions, to review the role of probiotics, their general use in insects reared for food and feed, and their interactions with the host microbiota. We review how bacterial strains have been selected for insect species reared for food and feed and discuss methods used to isolate and measure the effectiveness of a probiotic. We outline future perspectives on probiotic applications in mass-reared insects.
Reply to Jensen, O.K. On the Use of Quantitative Sensory Testing to Estimate Central Sensitization in Humans. Comment on “Schuttert et al. The Definition, Assessment, and Prevalence of (Human Assumed) Central Sensitisation in Patients with Chronic Low Back Pain: A Systematic Review. J. Clin. Med. 2021, 10, 5931”
Authors: Schuttert Ingrid , Timmerman Hans , Petersen Kristian K. , McPhee Megan E. , Arendt-Nielsen Lars , Reneman Michiel F. , Wolff André P.
Published: April 2022

We thank Dr. Jensen for his interest [...]
Effects in Israel of Arab and Jewish Ethnicity on Intracerebral Hemorrhage
Authors: Simaan Naaem , Filioglo Andrei , Cohen José E. , Lorberboum Yonatan , Leker Ronen R. , Honig Asaf
Published: April 2022

Intracerebral hemorrhages (ICH) characteristics reportedly differ between different ethnic groups. We aimed to compare the characteristics of Jewish and Arab ICH patients in Israel. Consecutive patients with primary ICH were included in a prospective institutional database. Demographics, vascular risk factors, clinical and radiological parameters were compared between Arab and Jewish ICH patients residing in Jerusalem. The study included 455 patients (311 Jews). Arab patients were younger (66.1 ± 13.4 vs. 72.2 ± 12.2 years, p < 0.001) and had higher rates of diabetes (60% vs. 29%, p < 0.001) and smoking (26% vs. 11%, p < 0.001). Arab patients had higher rates of deep ICH (74% vs. 62%, p = 0.01) and lower rates of lobar ICH (18% vs. 31%, p = 0.003). In a sub-analysis of deep ICH patients only, Arab patients were younger (64.3 ± 12.9 vs. 71.4 ± 11.8 years, p < 0.001) and less frequently male (56% vs. 68%, p = 0.042), with higher rates of diabetes (61% vs. 35%, p < 0.001) and smoking (31% vs. 14%, p < 0.001). In conclusion, the two ethnic populations in Israel differ in the causes and attributes of ICH. Heavy smoking and poorly controlled diabetes are commonly associated with deep ICH in the Arab population and may offer specific targets for secondary prevention in this population.
Serum Proteins, HMMR, NXPH4, PITX1 and THBS4; A Panel of Biomarkers for Early Diagnosis of Hepatocellular Carcinoma
Authors: Eun Jung Woo , Jang Jeong Won , Yang Hee Doo , Kim Jooyoung , Kim Sang Yean , Na Min Jeong , Shin Eunbi , Ha Jin Woong , Jeon Soyoung , Ahn Young Min , Park Won Sang , Nam Suk Woo
Published: April 2022

The high morbidity rate of hepatocellular carcinoma (HCC) is mainly linked to late diagnosis. Early diagnosis of this leading cause of mortality is therefore extremely important. We designed a gene selection strategy to identify potential secretory proteins by predicting signal peptide cleavage sites in amino acid sequences derived from transcriptome data of human multistage HCC comprising chronic hepatitis, liver cirrhosis and early and overt HCCs. The gene selection process was validated by the detection of molecules in the serum of HCC patients. From the computational approaches, 10 gene elements were suggested as potent candidate secretory markers for detecting HCC patients. ELISA testing of serum showed that hyaluronan mediated motility receptor (HMMR), neurexophilin 4 (NXPH4), paired like homeodomain 1 (PITX1) and thrombospondin 4 (THBS4) are early-stage HCC diagnostic markers with superior predictive capability in a large cohort of HCC patients. In the assessment of differential diagnostic accuracy, receiver operating characteristic curve analyses showed that HMMR and THBS4 were superior to α-fetoprotein (AFP) in diagnosing HCC, as evidenced by the high area under the curve, sensitivity, specificity, accuracy and other values. In addition, comparative analysis of all four markers and AFP combinations demonstrated that HMMR-PITX1-AFP and HMMR-NXPH4-PITX1 trios were the optimal combinations for reaching 100% accuracy in HCC diagnosis. Serum proteins HMMR, NXPH4, PITX1 and THBS4 can complement measurement of AFP in diagnosing HCC and improve identification of patients with AFP-negative HCC as well as discriminate HCC from non-malignant chronic liver disease.
Polypharmacotherapy in Psychiatry: Global Insights from a Rapid Online Survey of Psychiatrists
Authors: Ordak Michal , Tkacz Daria , Golub Aniela , Nasierowski Tadeusz , Bujalska-Zadrozny Magdalena
Published: April 2022

In recent years, an increase in the problem of polypharmacotherapy in psychiatric patients has been observed, including the widespread problem of groups of people taking new psychoactive substances. One reason for this problem may be the poor knowledge of pharmacological interactions in psychiatry. The aim of this study was to explore the opinions and knowledge of psychiatrists from around the world on various aspects related to polypharmacotherapy. A total of 1335 psychiatrists from six continents were included in the study. The respondents’ opinion on the problem of hepatotoxicity in psychiatry was also examined. The greatest discrepancy among psychiatrists from different continents in the answers given concerned the definition of polypharmacotherapy (p < 0.001) and the approach to hepatotoxicity (p < 0.001). It is noteworthy that only about 20% of the psychiatrists surveyed (p < 0.001) believe that polypharmacotherapy is associated with a higher rate of patients’ hospitalisations. The most commonly used type of polypharmacy by psychiatrists was antidepressants and antipsychotics. Most of them also stated that polypharmacy was associated with reduced patient compliance with the doctor’s recommendations related to taking medications due to the increased complexity of the therapy. The continent that diversified the analysed questions to the greatest extent was Africa. Future educational activities for trainee psychiatrists should include more discussion of polypharmacotherapy in psychiatry.
Oncologic and Long-Term Outcomes of Laparoscopic and Open Extended Cholecystectomy for Gallbladder Cancer
Authors: Lee Jong Woo , Kwon Jae Hyun , Lee Jung Woo
Published: April 2022

Laparoscopic surgery has been traditionally contraindicated for gallbladder cancer, but there have been few reports demonstrating the oncologic outcomes of this treatment. This study aimed to compare the technical feasibility and the long-term outcomes after laparoscopic versus open extended cholecystectomy for gallbladder cancer. Between January 2011 and December 2018, 44 patients with gallbladder cancer who underwent extended cholecystectomy were included in this study, with 20 patients in the laparoscopic group and 24 patients in the open group. Perioperative outcomes, overall survival (OS), and recurrence-free survival (RFS) were retrospectively analyzed. There were no significant differences (p > 0.05) between the two groups in terms of perioperative outcomes, including blood loss, postoperative complications, R0 resection, and the number of lymph nodes retrieved. Patients in the laparoscopic group showed similar OS compared to the open group (5 year tumor-specific OS rate: 84.7% vs. 62.5%; p = 0.125). On subgroup analysis of patients with stage T2 and N0 disease, the laparoscopic group showed better OS (T2: 90.9% vs. 75.0%, p = 0.256; N0: 100.0% vs. 76.5%, p = 0.028). There was no difference in terms of RFS (3 year RFS: 74.4% vs. 64%; p = 0.571) and locoregional recurrence (10.0% vs. 16.9%, p = 0.895) between the two groups. There was no port-site recurrence in the laparoscopic group. This study suggests that laparoscopic extended cholecystectomy might be not inferior to open surgery in terms of oncologic safety or early and long-term outcomes in patients with early gallbladder cancer.
Contemporary Use of ICSI and Epigenetic Risks to Future Generations
Authors: Sciorio Romualdo , Esteves Sandro C.
Published: April 2022

Since the birth of Louise Brown in 1978 via IVF, reproductive specialists have acquired enormous knowledge and refined several procedures, which are nowadays applied in assisted reproductive technology (ART). One of the most critical steps in this practice is the fertilization process. In the early days of IVF, a remarkable concern was the unpleasant outcomes of failed fertilization, overtaken by introducing intracytoplasmic sperm injection (ICSI), delineating a real breakthrough in modern ART. ICSI became standard practice and was soon used as the most common method to fertilize oocytes. It has been used for severe male factor infertility and non-male factors, such as unexplained infertility or advanced maternal age, without robust scientific evidence. However, applying ICSI blindly is not free of potential detrimental consequences since novel studies report possible health consequences to offspring. DNA methylation and epigenetic alterations in sperm cells of infertile men might help explain some of the adverse effects reported in ICSI studies on reproductive health in future generations. Collected data concerning the health of ICSI children over the past thirty years seems to support the notion that there might be an increased risk of epigenetic disorders, congenital malformations, chromosomal alterations, and subfertility in babies born following ICSI compared to naturally conceived children. However, it is still to be elucidated to what level these data are associated with the cause of infertility or the ICSI technique. This review provides an overview of epigenetic mechanisms and possible imprinting alterations following the use of ART, in particular ICSI. It also highlights the sperm contribution to embryo epigenetic regulation and the risks of in vitro culture conditions on epigenetic dysregulation. Lastly, it summarizes the literature concerning the possible epigenetic disorders in children born after ART.
Strong Cellular Immune Response, but Not Humoral, against SARS-CoV-2 in Oncohematological Patients with Autologous Stem Cell Transplantation after Natural Infection
Authors: Vigón Lorena , Sánchez-Tornero Adrián , Rodríguez-Mora Sara , García-Pérez Javier , Corona de Lapuerta Magdalena , Pérez-Lamas Lucía , Casado-Fernández Guiomar , Moreno Gemma , Torres Montserrat , Mateos Elena , Murciano-Antón María Aránzazu , Alcamí José , Pérez-Olmeda Mayte , López-Jiménez Javier , García-Gutiérrez Valentín , Coiras Mayte ,   on behalf of Multidisciplinary Group of Study of COVID-19 (MGS-COVID)
Published: April 2022

Oncohematological patients show a low immune response against SARS-CoV-2, both to natural infection and after vaccination. Most studies are focused on the analysis of the humoral response; therefore, the information available about the cellular immune response is limited. In this study, we analyzed the humoral and cellular immune responses in nine individuals who received chemotherapy for their oncohematological diseases, as well as consolidation with autologous stem cell transplantation (ASCT), after being naturally infected with SARS-CoV-2. All individuals had asymptomatic or mild COVID-19 and were not vaccinated against SARS-CoV-2. These results were compared with matched healthy individuals who also had mild COVID-19. The humoral response against SARS-CoV-2 was not detected in 6 of 9 oncohematological individuals prior to ASCT. The levels of antibodies and their neutralization capacity decreased after ASCT. Conversely, an enhanced cytotoxic activity against SARS-CoV-2-infected cells was observed after chemotherapy plus ASCT, mostly based on high levels of NK, NKT, and CD8+TCRγδ+ cell populations that were able to produce IFNγ and TNFα. These results highlight the importance of performing analyses not only to evaluate the levels of IgGs against SARS-CoV-2, but also to determine the quality of the cellular immune response developed during the immune reconstitution after ASCT.
Vaginal Dinoprostone Insert versus Cervical Ripening Balloon for Term Induction of Labor in Obese Nulliparas—A Randomized Controlled Trial
Authors: Lauterbach Roy , Ben Zvi Dikla , Dabaja Haneen , Zidan Ragda , Justman Naphtali , Vitner Dana , Beloosesky Ron , Ghanem Nadir , Ginsberg Yuval , Zipori Yaniv , Weiner Zeev , Khatib Nizar
Published: April 2022

Data regarding the preferred induction method in women with obesity is scarce. The current study was aimed at comparing pharmacological and mechanical induction in this population. This prospective randomized controlled trial was conducted between 2016–2020, in nulliparas with a pre-pregnancy body mass index >30. Inclusion criteria were singleton-term pregnancies, bishop score < 5, and indication for induction. Patients were randomized to induction by a cervical ripening balloon (CRB) or a 10 mg vaginal dinoprostone insert. The primary outcome was delivery rate within 24 h. Secondary outcomes included time to delivery, cesarean section rate, maternal and neonatal outcomes, satisfaction, and anxiety. The study population comprised of 83 women in the CRB group and 81 in the dinoprostone group. There was a significant difference in delivery rates within 24 h and time to delivery between the dinoprostone and CRB groups (45% vs. 71%, p = 0.017 and 49.3 ± 6.8 h vs. 23.5 ± 5.9 h, p = 0.003, respectively). There were no differences in cesarean delivery rates or maternal and neonatal outcomes, though CRB induction was associated with a significantly lower rate of tachysystole. Induction with CRB was accompanied by higher satisfaction and lower anxiety. In summary, CRB induction is associated with shorter time to delivery, higher satisfaction, and lower anxiety compared to PGE2 in women with obesity, without compromising maternal or neonatal outcomes.
Ophthalmological Impairments at Five and a Half Years after Preterm Birth: EPIPAGE-2 Cohort Study
Authors: Chapron Thibaut , Pierrat Véronique , Caputo Georges , Letouzey Mathilde , Kermorvant-Duchemin Elsa , Abdelmassih Youssef , Beaumont William , Barjol Amandine , Le Meur Guylene , Benhamou Valérie , Marchand-Martin Laetitia , Ancel Pierre-Yves , Torchin Héloïse
Published: April 2022

We report the 51/2 year prevalence of visual and oculomotor impairments in preterm children born at 24–34 weeks’ gestation (WG) using the population-based cohort study EPIPAGE-2, set in France, 2011. The main outcomes were imputed prevalence of refractive errors (REs), strabismus, and binocular visual acuity (VA). Children were clinically assessed by specially trained pediatricians. The population was also analyzed in terms of cerebral palsy at 51/2 years (no CP, stage 1, stage 2, or stage 3–5) and retinopathy of prematurity in the neonatal period (no ROP, stage 1 or 2, or severe ROP). Among the 4441 children included, 2718 (weighted percentage 58.7%) were clinically assessed. REs were reported in 43.1% (95% confidence interval 37.6–48.4), 35.2% (32.7–37.6), and 28.4% (25.0–31.8) of children born at 24–26, 27–31, and 32–34 WG (p < 0.01), respectively; strabismus rates were 19.5% (14.6–24.4), 14.8% (12.9–16.7), and 8.3% (6.2–10.4) (p < 0.001), respectively. Moderate/severe visual deficiencies (VA < 3.2/10) were present in 1.7% (0.2–3.3) of children born at 24–26 WG, and in less than 1% in other groups. A suboptimal VA 5/10–6.3/10 was measured in 40.6% (35.3–45.8) of children born at 24–26 WG, 35.8% (33.5–38.1) at 27–31 WG, and 33.7% (30.4–37.0) at 32–34 WG. CP and ROP were associated with strabismus and RE. The association between CP and VA was strong, while it was not observed for ROP. In this large cohort of preterm-born children, we found a high prevalence of RE and strabismus regardless of WG, supporting the need for specific attention in this population. High prevalence of suboptimal VA could be challenging for these children at the age of reading and writing acquisition.
Patent Blue V Dye Adsorption by Fresh and Calcined Zn/Al LDH: Effect of Process Parameters and Experimental Design Optimization
Authors: Machrouhi Aicha , Taoufik Nawal , Elhalil Alaâeddine , Tounsadi Hanane , Rais Zakia , Barka Noureddine
Published: April 2022

This work focuses on the adsorptive removal of patent blue V (PBV) dye from aqueous solution by Zn/Al layered double hydroxide in fresh (LDH) and calcined (CLDH) forms. The material was synthesized via coprecipitation and samples were characterized by XRD, FTIR and TGA-DTA. Dye retention was evaluated under different experimental conditions of contact time, pH, adsorbent dosage, temperature and initial dye concentration. Experimental results show that highest adsorption capacity occurred at acidic medium. Kinetics data were properly fitted with the pseudo-second-order model. Equilibrium data were best correlated to Langmuir model with maximum monolayer adsorption capacities of 185.40 and 344.37 mg/g, respectively, for LDH and CLDH. The process was endothermic and spontaneous in nature. Based on the preliminary study, full factorial experimental design (24) was used for the optimization of the effect of solution pH, adsorbent dose, initial dye concentration and the calcination. Thus, the optimal conditions to reach high equilibrium adsorption capacity were achieved at pH of 5, adsorbent dosage of 0.1 g/L, and initial dye concentration of 15 mg/L by CLDH.
Microbial Adhesion to Dental Polymers for Conventional, Computer-Aided Subtractive and Additive Manufacturing: A Comparative In Vitro Study
Authors: Arutyunov Sergey , Kirakosyan Levon , Dubova Lubov , Kharakh Yaser , Malginov Nikolay , Akhmedov Gadzhi , Tsarev Viktor
Published: April 2022

Modern structural materials are represented by a variety of polymer materials used for dental patients’ rehabilitation. They differ not only in physico-chemical properties, but also in microbiological properties, which is one of the reasons why these materials are chosen. The study focused on the microbial adhesion of clinical isolates of normal (5 types), periodontopathogenic (2 types), and fungal (2 types) microbiotas to various materials based on polymethylmethacrylate (PMMA) intended for traditional (cold-cured and hot-cured polymers), computer-aided subtractive and additive manufacturing. A comparative analysis was carried out on the studied samples of polymer materials according to the microorganisms’ adhesion index (AI). The lowest level of microorganisms’ AI of the three types of microbiotas was determined in relation to materials for additive manufacturing. The AI of hot-cured polymers, as well as materials for subtractive manufacturing, corresponded to the average level. The highest level of microorganisms’ adhesion was found in cold-cured polymers. Significant differences in AI for materials of the same technological production type (different manufacturers) were also determined. The tendency of significant differences in the indicators of the microorganisms’ adhesion level for the studied polymer materials on the basis of the type of production technology was determined.
Lenses of Iran’s Role in Syria’s Conflict through a Comparison between BBC and Sputnik: News Approaches from Revisionist, Multilateral, and Counter-Hegemonic Discourse
Authors: Gharayagh-Zandi Davoud
Published: April 2022

Syria’s domestic dissent created the foundation for the country’s civil conflict, where foreign actors have favored the different sides since the 2011 Arab Spring. Iran was invited to play an active role in the conflict owing to the declining fortunes of the Syrian government. Iran welcomed the opportunity and understood that it suited its foreign policy priority to endorse the counter-hegemony approach in the MENA region in addition to maintaining the country’s political stability. It is essential to analyze how international media outlets covered Iran’s role in Syria. This study addressed the news coverage provided by Great Britain’s BBC and Russia’s Sputnik—two news agencies from two distinct geopolitical sides—both of which have Persian news outlets covering Iran’s role in the Syrian conflict. The foreign policy goals pursued by Iran aim at two different approaches: revisionism and multilateral partnership. Conducting a critical discourse analysis review of the news sources this study sought to demonstrate that the mass media attempt to engineer public consent in armed conflicts. According to the findings, the news agencies attempted to influence the policy preferences to adopt a peaceful architecture or try to induce a consent-driven perception in line with the foreign policy priorities set by their countries of origin.
Change of Optical Coherence Tomography Morphology and Associated Structural Outcome in Diabetic Macular Edema after Ranibizumab Treatment
Authors: Chen Nan-Ni , Lai Chien-Hsiung , Lee Chai-Yi , Kuo Chien-Neng , Chen Ching-Lung , Huang Jou-Chen , Wu Pei-Chen , Wu Pei-Lun , Chen Chau-Yin
Published: April 2022

(1) Background: To investigate the correlation between therapeutic outcome and morphologic changes for diabetic macular edema (DME) after intravitreal injection of ranibizumab (IVIR). (2) Methods: This retrospective study included 228 eyes received IVIR for DME. Each participant was traced for two years after the initial IVIR, while the data of ophthalmic examination, optical coherence tomography (OCT) image, and systemic diseases were collected. The study population was categorized into different subgroups according to the existence of OCT morphologic change and the initial OCT morphologic pattern, including diffuse retinal thickening (DRT), cystoid macular edema (CME), serous retinal detachment (SRD), and vitreomacular interface abnormalities (VMIAs). The primary outcomes were the baseline best-corrected visual acuity (BCVA) and central macular thickness (CMT) during a two-year study period. The distribution of OCT morphologic change and its relation to primary outcome were analyzed. (3) Results: Comparing the 42 eyes (18.4%) with OCT morphological changes to another 186 eyes (81.6%) without such alteration, the former showed a poorer baseline BCVA (0.84 ± 0.39 vs. 0.71 ± 0.36, p = 0.035), worse final BCVA (0.99 ± 0.44 vs. 0.67 ± 0.30, p = 0.001), and thicker final CMT (354.21 ± 89.02 vs. 305.33 ± 83.05, p = 0.001). Moreover, the VMIA developed in 14.9% of all DME patients presenting the most common morphologic change among DRT, CME, and SRD. Besides, the presence of stroke was independently correlated to the morphologic change (adjusted odds ratio [aOR]: 6.381, 95% confidence interval (CI): 1.112–36.623, p = 0.038). (4) Conclusions: The change of OCT morphology in DME patients receiving IVIR was correlated to worse structural and visual outcome while the formation of VMIA most commonly occurred after initial treatment.
Development and Validation of a Non-Invasive, Chairside Oral Cavity Cancer Risk Assessment Prototype Using Machine Learning Approach
Authors: Shimpi Neel , Glurich Ingrid , Rostami Reihaneh , Hegde Harshad , Olson Brent , Acharya Amit
Published: April 2022

Oral cavity cancer (OCC) is associated with high morbidity and mortality rates when diagnosed at late stages. Early detection of increased risk provides an opportunity for implementing prevention strategies surrounding modifiable risk factors and screening to promote early detection and intervention. Historical evidence identified a gap in the training of primary care providers (PCPs) surrounding the examination of the oral cavity. The absence of clinically applicable analytical tools to identify patients with high-risk OCC phenotypes at point-of-care (POC) causes missed opportunities for implementing patient-specific interventional strategies. This study developed an OCC risk assessment tool prototype by applying machine learning (ML) approaches to a rich retrospectively collected data set abstracted from a clinical enterprise data warehouse. We compared the performance of six ML classifiers by applying the 10-fold cross-validation approach. Accuracy, recall, precision, specificity, area under the receiver operating characteristic curve, and recall–precision curves for the derived voting algorithm were: 78%, 64%, 88%, 92%, 0.83, and 0.81, respectively. The performance of two classifiers, multilayer perceptron and AdaBoost, closely mirrored the voting algorithm. Integration of the OCC risk assessment tool developed by clinical informatics application into an electronic health record as a clinical decision support tool can assist PCPs in targeting at-risk patients for personalized interventional care.
Interrelationship of Gut Microbiota, Obesity, Body Composition and Insulin Resistance in Asians with Type 2 Diabetes Mellitus
Authors: Pai Che-Sheng , Wang Cheng-Yuan , Hung Wei-Wen , Hung Wei-Chun , Tsai Hui-Ju , Chang Chen-Chia , Hwang Shang-Jyh , Dai Chia-Yen , Ho Wen-Yu , Tsai Yi-Chun
Published: April 2022

Metabolic syndrome (MS) has been an important health issue in the world, and insulin resistance (IR) is one of the characteristics of MS, increasing the risk for the onset and poor prognosis of type 2 diabetes mellitus (T2D). However, the interactional effect of obesity or abnormal body composition on the correlation between gut microbiota and IR in T2D patients is not well-explored. This cross-sectional study used a body composition monitor to evaluate lean tissue mass and fat tissue mass. IR was calculated using homeostatic model assessment-insulin resistance (HOMA-IR). Eight pairs of 16S rRNA gene primers specific to Firmicutes, Bacteroidetes, Clostridium leptum group, Faecalibacteriumprausnitzii, B acteroides, Bifidobacterium, Akkermansia muciniphila, and Escherichia coli were utilized to measure their abundance by qPCR. One hundred and fifty-four T2D patients were enrolled and stratified by the median HOMA-IR (2.5) and body mass index (BMI) of 25 kg/m2. A lower abundance of A. muciniphila was found in T2D patients with high HOMA-IR and BMI respectively. HOMA-IR and BMI had a synergistic effect on the reduction of the abundance of A. muciniphila. After adjusting metabolic factors, the low abundance of A. muciniphila significantly increased the risk for greater severity of IR. Furthermore, the negative correlation between A. muciniphila and IR was only found in T2D patients with high lean tissue. In conclusion, decreased abundance of fecal A. muciniphila enhanced the severity of IR in Asians with T2D, especially those having lean mass, and this significant relationship was independent of obesity.
Risk of Burning Mouth Syndrome in Patients with Migraine: A Nationwide Cohort Study
Authors: Kim Dong-Kyu , Lee Hyun-Joo , Lee Il Hwan , Lee Jae-Jun
Published: April 2022

Migraine is a common neurological disease that causes a variety of symptoms, most notably throbbing, which is described as a pulsing headache on one side of the head. Burning mouth syndrome (BMS) is defined as an intra-oral burning sensation. Currently, no medical or dental cause has been identified for BMS. Interestingly, neuropathic pain is a characteristic feature of BMS; however, it remains unclear whether migraine can cause BMS. We aimed to identify the association of migraine with the risk of developing BMS. We used a representative nationwide cohort sample of approximately 1 million patients from 2002 to 2013 to investigate the prospective association between migraine and BMS. A total of 4157 migraine patients (migraine group) and 16,628 patients without migraine (comparison group) were enrolled after 1:4 propensity score matching. The overall incidence of BMS was significantly higher in the migraine group (0.15 per 1000 person-years) than in the comparison group (0.05 per 1000 person-years). The adjusted HR for patients with migraine who reported BMS events during the 10-year follow-up period was 2.96 (95% confidence interval, 1.02–8.56), after adjusting for other covariates. However, in the subgroup analysis, the adjusted HR for BMS events did not show a significant difference between the migraine and comparison group according to sex, age, and comorbidities. This study suggests that migraine is associated with an increased incidence of BMS. Therefore, clinicians should be attentive to detect BMS at an early stage when treating patients with migraine.
The Impact of Fashion Brand Sustainability on Consumer Purchasing Decisions
Authors: Mandarić Doroteja , Hunjet Anica , Vuković Dijana
Published: April 2022

The focus of this confirmatory research was on consumer attitudes towards the sustainability of fashion brands and how these attitudes influence their purchasing decisions. The aim was to explore if the gap between attitudes and purchasing behaviour was present within Croatian consumers to the same extent as previous research has shown. A survey was conducted of 263 respondents with purchasing power to examine their perception, awareness of, and attitudes towards sustainability and eco-fashion as consumers. The data collected were analysed using descriptive statistics and correlation analysis. The results suggest that participants have a positive attitude towards the sustainability of fashion brands. Moreover, a positive correlation was found between the importance of fashion brand sustainability and consumers’ decisions to buy sustainable clothing products. However, the sustainability of a fashion brand or product is among the least important factors in their purchasing decision. This could mean that their positive attitude may not necessarily be reflected in actual purchasing behaviour, which is consistent with previous research. The results of this study provide a framework for a greater understanding of the various factors that may influence consumer behaviour, such as the sustainability of a fashion brand or product, potentially facilitating the development of relevant strategies in the fashion industry and changing the way fashion works and is perceived in the future.
Does Handling for Public Talks in Zoos Affect the Behaviour of Captive Mexican Red-Kneed Spiders Brachypelma hamorii?
Authors: Gresham Charlotte , Mathews Fiona , Ferguson Amanda , Mitchell Jamie , Clifforde Lisa , Clarke Dave , Rowden Lewis J.
Published: April 2022

Zoos include invertebrates in visitor interaction sessions to educate and spread conservation messages to the public. Yet, the welfare implications of these encounters on invertebrates are unstudied. Empirical studies reveal negative effects of handling on vertebrate species, thus providing reason to investigate impacts on invertebrates. Mexican red-kneed spiders Brachypelma hamorii are regularly handled by keepers for public talks at the Zoological Society of London, London Zoo. This study investigates whether handling affects the spiders’ 24-h activity and enclosure usage. Three spiders were filmed under infrared light for 24 h following being handled, and on control (no-handling) days. The proportion of time that spiders spent under cover or exhibiting locomotion, limb-interaction, and object-interaction behaviour was recorded using instantaneous scan sampling. The spiders spent, on average, significantly more time under cover (7.8% increase) and exhibited significantly more limb-interaction behaviour (1.4% increase) on handling days. Handling for public talks therefore affects the behaviour and enclosure use of these captive Brachypelma hamorii. Although it is not yet possible to infer welfare implications, the presence of these behavioral responses suggests that protocols to monitor the behaviour of invertebrates following handling should be developed, and further behavioral studies are warranted to validate potential stress indices.
Application of Cmic/Corg in the Soil Fertility Evaluation of Typical Forests in the Yulin Sandy Area
Authors: Wang Yue , Wang Shan , Zhou Chun-Sheng , Chi Wen-Feng
Published: April 2022

The microbial quotient (Cmic/Corg) has been used extensively to evaluate agriculture soil fertility, but the microbial sensitivity should be considered during the forestry process. Therefore, the objective of this study was to examine a soil fertility evaluation method applied to four vegetation types in the Mu Us Sandland in northwestern China, using the relationship between the Cmic/Corg ratio and soil moisture, and soil temperature under the premise of microbial diversity. The final predictive value was C. microphylla (0.2198) > P. sylvestris (0.2175) > P. tabulaeformis (0.0872) > S.psammophila (0.0767). We verified the evaluation results using two traditional methods, the back-propagation (BP) artificial neural network model and principal component analysis, which are widely used to evaluate soil quality based on the soil nutrient concentration. The results were the same as the Cmic/Corg predictions. We conclude that when the soil microbes are used in soil quality evaluations, the changing pattern should be fully considered.
Ecosystem Stability Assessment of Yancheng Coastal Wetlands, a World Natural Heritage Site
Authors: Tian Peng , Cao Luodan , Li Jialin , Pu Ruiliang , Liu Yongchao , Zhang Haitao , Wang Caiyi
Published: April 2022

By evaluating the stability of coastal wetland ecosystems, health conditions of regional ecosystems can be revealed and the sustainable development of coastal wetlands can be promoted. Coastal wetlands have been scarcely involved in present ecosystem stability evaluation studies, these being performed with relatively simple evaluation data sources. Therefore, in this research, a comprehensive and representative ecosystem stability evaluation index system was constructed by using the pressure-state-response model and multi-source datasets from perspectives of internal and external environmental changes of the Yancheng coastal wetlands, Jiangsu, China. The analysis results indicated that: (1) The ecosystem stability of the Yancheng coastal wetlands was at an early warning stage, and all segments except the Binhai segment (relatively stable) were in an early warning state. (2) In the criterion layer, the Dafeng District and the whole Yancheng District were faced with the highest pressure, followed by the Dongtai, Xiangshui and Binhai segments, successively. The Sheyang segment reached the highest state level, followed by the Binhai, Xiangshui and Dafeng segments in succession. (3) In the factor layer, the whole Yancheng District was faced with high resource and socioeconomic double pressures, with a poor water quality state and relatively low environmental pressure; favorable soil, biological and landscape states; and positive response to wetland protection. Various factors varied from county to county. (4) In the index layer, the ecosystem stability of the Yancheng coastal wetlands was significantly influenced by the invasion of alien species, change rate of natural wetland area (D32), change rate of artificial wetland area, increment of aquafarm area, intensity of fertilizer application and coverage of dominant vegetations. The novel significance of this research lies in enriching global coastal wetlands ecosystem stability evaluation investigations by providing a typical case study.
Microvascular and Morphologic Changes of the Macula over Lifetime
Authors: Lever Mael , Chen Ying , Glaser Moritz , Unterlauft Jan Darius , Lommatzsch Claudia , Bechrakis Nikolaos E. , Böhm Michael R. R.
Published: April 2022

In ocular, neurologic, and cardiovascular diseases, macular segmentation data from spectral-domain optical coherence tomography (SD-OCT) provide morphologic, and OCT-angiography (OCTA) results give microvascular information about the macula. Age was shown to influence both methods’ measurements. To further characterize this association, macular SD-OCT and OCTA changes were investigated in a population of juvenile, adult, and older individuals. Macular segment thickness and superficial (SCP) and deep plexus (DCP) vascular density (VD) of 157 healthy individuals aged 10–79 years were analyzed retrospectively. One-way analysis of variance (ANOVA) was used to compare age groups. The association between macular segmentation and OCTA parameters and between these and age was evaluated using linear regression. ANOVA and linear regression analysis showed a thickness decrease in the whole macular and in the ganglion cell and inner plexiform layers with age. While the foveal avascular zone area remained constant between age groups, VD of the SCP and DCP also decreased with age. In multiple linear regression, SCP and DCP VD were associated with inner macular segment thickness in an age-independent way. To conclude, the age-related microvascular and morphological changes in the macula described in this study can contribute to improving the understanding of macular aging processes and better interpreting OCT(A) results in healthy individuals and patients suffering from various retinal diseases.
The Fate of Leydig Cells in Men with Spermatogenic Failure
Authors: Adamczewska Daria , Słowikowska-Hilczer Jolanta , Walczak-Jędrzejowska Renata
Published: April 2022

The steroidogenic cells in the testicle, Leydig cells, located in the interstitial compartment, play a vital role in male reproductive tract development, maintenance of proper spermatogenesis, and overall male reproductive function. Therefore, their dysfunction can lead to all sorts of testicular pathologies. Spermatogenesis failure, manifested as azoospermia, is often associated with defective Leydig cell activity. Spermatogenic failure is the most severe form of male infertility, caused by disorders of the testicular parenchyma or testicular hormone imbalance. This review covers current progress in knowledge on Leydig cells origin, structure, and function, and focuses on recent advances in understanding how Leydig cells contribute to the impairment of spermatogenesis.
Influence of the Temperature-Strain Parameters on the Structure Evolution and Carbide Transformations of Cr-Ni-Ti Stainless Steel
Authors: Rudskoi Andrei , Kodzhaspirov Georgii
Published: April 2022

Influence of strain accumulation schedule during hot rolling, under the thermomechanical control process (TMCP) on the structure evolution and carbide transformations of Cr-Ni-Ti austenitic stainless steel, is studied. The cellular, fragmented dislocation substructure and dynamically recrystallized (DRX) structure are observed in the steel with different strain accumulation schedules. It was found that the strain accumulation schedule, especially fractionality, affects the work-hardening and softening behavior quite significantly. The role of the strain accumulation schedule on the fragmented substructure and DRX structure evolution as well as carbide transformations and the relationship between the microstructure changes due to TMCP and the mechanical properties of studied steel, involving the recent ideas of the physics of large plastic strains, are considered.
Bond Behavior of Concrete-Filled Steel Tube Mega Columns with Different Connectors
Authors: Alemayehu Robel Wondimu , Bae Jaehoon , Ju Young K. , Park Min Jae
Published: April 2022

Concrete-filled steel tubes (CFSTs) are widely used in construction. To achieve composite action and take full advantage of the two materials, strain continuity at the steel–concrete interface is essential. When the concrete core and steel tube are not loaded simultaneously in regions such as beam or brace connections to the steel tubes of a CFST column, the steel–concrete bond plays a crucial role in load transfer. This study uses a validated finite-element model to investigate the bond-slip behavior between the steel tube and concrete in square CFST mega columns through a push-out analysis of eleven 1.2- × 1.2-m mega columns. The bond-slip behavior of CFST mega columns with and without mechanical connectors, including shear studs, rib plates, and connecting plates, is studied. The finite-element results indicate that the mechanical connectors substantially increased the maximum bond stress. Among the analyzed CFST mega columns, those with closely spaced shear studs and rib plate connectors with circular holes exhibited the highest bond stress, followed by plate connectors and widely spaced shear stud connectors. In the case of shear stud connectors, the stud diameter and spacing influenced the bond behavior more than the stud length. As the stud spacing decreased, the failure mode shifted from studs shearing off to outward buckling of the steel tube.
A New Insight on Phased Array Ultrasound Inspection in MIG/MAG Welding
Authors: Alonso José , Pavón Santiago , Vidal Juan , Perdigones José , Carpena Isaac
Published: April 2022

Weldment inspection is a critical process in the metal industry. It is first conducted visually, then manually and finally using instrumental techniques such as ultrasound. We made one hundred metal inert/active gas (MIG/MAG) weldments on plates of naval steel S275JR+N with no defects, and inducing pores, slag intrusion and cracks. With the objective of the three-dimensional reconstruction of the welding defects, phased array ultrasound inspections were carried out. Error-free weldment probes were used to provide the noise level. The results can be summarized as follows. (i) The top view obtained from the phased array provided no conclusive information about the welding defects. The values of the echo amplitudes were about 70 mV for pores and cracks, and greater than 150 mV for slag intrusion, all of which showed great variability. (ii) The sectional data did not lie at the same depths and they needed to be interpolated. (iii) The interpolated sectional views, or C-scans, allowed the computation of top views at any depth, as well as the three-dimensional reconstruction of the defects. (iv) The use of the simplest tool, consisting of the frequency histogram and its statistical moments, was sufficient to classify the defects. The mean echo amplitudes were 33 mV for pores, 72.16 mV for slag intrusion and 43.19 mV for cracks, with standard deviations of 8.84 mV, 24.64 mV and 12.39 mV, respectively. These findings represent the first step in the automatic classification of welding defects.
Effects of Surfactants on Zein Cast Films for Simultaneous Delivery of Two Hydrophilic Active Components
Authors: Wei Dongwei , Zhou Fanhui , Wang Hongdi , Liu Guijin , Fang Jun , Jiang Yanbin
Published: April 2022

In order to prepare edible films with outstanding antimicrobials and antioxidants utilized in applications of food and pharmaceutics, in this study, effects of surfactants on zein cast films for simultaneous delivery of lysozyme (LY) and ascorbic acid (AA) were investigated, where sodium alginate (SA), soy lecithin (SL), and Pluronic f-68 (PF-68) were selected as surfactants. FT-IR tests indicated that SL or PF-68 dramatically changed secondary structure of zein composite films, which heightened the irregularity of the composite film and inhibited LY crystallization. Mechanical tests showed that highly flexible films exhibiting elongations between 129% and 157% were obtained when adding PF-68. Compared with the film without emulsifier, zein film containing SL and PF-68 showed approximately 7.51 and 0.55 times lower initial release rates for LY and AA respectively, which significantly improved the controlled release and heightened the anti-microbial and anti-oxidant activities of the film. Finally, emulsified mechanisms of the surfactants in zein films were proposed.
3D-Printed Double-Helical Biodegradable Iron Suture Anchor: A Rabbit Rotator Cuff Tear Model
Authors: Liu Wen-Chih , Chang Chih-Hau , Chen Chung-Hwan , Lu Chun-Kuan , Ma Chun-Hsien , Huang Shin-I , Fan Wei-Lun , Shen Hsin-Hsin , Tsai Pei-I , Yang Kuo-Yi , Fu Yin-Chih
Published: April 2022

Suture anchors are extensively used in rotator cuff tear surgery. With the advancement of three-dimensional printing technology, biodegradable metal has been developed for orthopedic applications. This study adopted three-dimensional-printed biodegradable Fe suture anchors with double-helical threads and commercialized non-vented screw-type Ti suture anchors with a tapered tip in the experimental and control groups, respectively. The in vitro study showed that the Fe and Ti suture anchors exhibited a similar ultimate failure load in 20-pound-per-cubic-foot polyurethane foam blocks and rabbit bone. In static immersion tests, the corrosion rate of Fe suture anchors was 0.049 ± 0.002 mm/year. The in vivo study was performed on New Zealand white rabbits and SAs were employed to reattach the ruptured supraspinatus tendon. The in vivo ultimate failure load of the Fe suture anchors was superior to that of the Ti suture anchors at 6 weeks. Micro-computed tomography showed that the bone volume fraction and bone surface density in the Fe suture anchors group 2 and 6 weeks after surgery were superior, and the histology confirmed that the increased bone volume around the anchor was attributable to mineralized osteocytes. The three-dimensional-printed Fe suture anchors outperformed the currently used Ti suture anchors.