bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Optimal Current Trajectory Control Strategy of IPMSM Considering the Cross Saturation Effects
Authors: Li Huimin , Gao Jian , Huang Shoudao , Fan Peng
Published: September 2017

Abstract: The nonlinearity and uncertain variation of machine parameters are always caused by cross coupling and magnetic saturation effects, which are easily neglected in the conventional control strategy. In this paper, a current trajectory control strategy (CTCS) is proposed to take the cross coupling and magnetic saturation effects into account under voltage and current constraints. It can be considered as a calculating method considering parameter variation and separating among each iteration step which treats the calculated result of the former step as the initial value of the next step. At first, the torque command is translated into the current reference. Then, the increments between the target value and real value of the torque and the voltage are respectively calculated, which are subsequently converted into the current modification vector in did, diq framework for further analysis. In order to take the influence caused by cross coupling and magnetic saturation effects on the CTCS into consideration, self and mutual inductances are analyzed by finite element analysis (FEA). The results of the simulation and experiment show that the rapid response and robustness on reference speed variation could be achieved by employing the proposed CTCS, and the seamless switching between the constant torque and flux-weakening operation can also be realized.
Does the Slope of the Self-thinning Line Remain a Constant Value across Different Site Qualities?—An Implication for Plantation Density Management
Authors: Ge Fangxing , Zeng Weisheng , Ma Wu , Meng Jinghui
Published: September 2017

The self-thinning rule is regarded as one of the most important principles in plantation management. This rule, involving the assumption of a constant slope coefficient, has been universally applied when regulating stand density. In this study, we hypothesized that the slope coefficient can change significantly with changes in site quality. To test this hypothesis, we first grouped forest plots into 5 categories based on site index. Second, we produced the self-thinning line represented by the Reineke function for each of the 5 site categories, selecting fully stocked plots using reduced major axis regression. Third, the slope coefficients for the different categories were tested for significant differences. The results indicated that in general, the slope was significantly different with different site quality. In addition, we observed that the slope of the self-thinning line exhibited a steeper trend for sites of lower quality, which indicated increased self-thinning or reduced self-tolerance. Finally, we concluded that it is imperative to produce specific self-thinning lines for different site quality categories.
New Occurrence of Pyroxenites in the Veria-Naousa Ophiolite (North Greece): Implications on Their Origin and Petrogenetic Evolution
Authors: Rogkala Aikaterini , Petrounias Petros , Tsikouras Basilios , Hatzipanagiotou Konstantin
Published: September 2017

The Veria-Naousa ophiolite represents a dismembered unit in north Greece, which includes variably serpentinised lherzolite and harzburgite, locally intruded by a sparse network of dykes or thin layers of websterite and olivine-orthopyroxenite composition. The websterite and the olivine-orthopyroxenite show abundant petrographic and geochemical evidence (relic olivines with mantle affinities, Cr-rich spinels, low Al2O3, depletions in incompatible elements, and concave upwards rare earth element patterns) that they comprise replacive bodies from refractory subarc mantle precursors. The occurrence of these pyroxenites in dykes implies that channelled percolation of melts account for their replacive character. High CaO/Al2O3, low Zr and crystallisation of diopside suggest that a melt of ankaramitic/carbonatitic composition percolated in lherzolite replacing porphyroclastic olivine and forming the pyroxenes in the websterite. At a shallower level, harburgites were impregnated by boninitic melts (inferred by U-shape rare earth element patterns and very rich in Cr spinels) triggering the replacement of porphyroclastic olivine by orthopyroxene for the formation of olivine-orthopyroxenite. These peritectic replacements of olivine commonly occur in a mantle wedge regime. The peculiar characteristics of the Veria-Naousa pyroxenites with LREE and compatible elements enrichments resemble the subarc pyroxenites of Cabo Ortegal implying a similar environment of formation. Whole-rock and mineralogical (spinel and clinopyroxene) compositions are also in favour of a backarc to arc environment. It is recommended that the evolution of the Veria-Naousa pyroxenites record the evolution of the subarc region and the opening of a backarc basin in a broad SSZ setting in the Axios Zone of eastern Greece.
Frailty and Its Contributory Factors in Older Adults: A Comparison of Two Asian Regions (Hong Kong and Taiwan)
Authors: Yu Ruby , Wu Wan-Chi , Leung Jason , Hu Susan C. , Woo Jean
Published: September 2017

This study aimed to compare the prevalence of frailty across three Chinese populations: Hong Kong, Taiwan-urban and Taiwan-rural. Contributing factors to disparities in frailty were also examined. Data were derived from the Osteoporotic Fractures in Men (MrOs) and Women (MsOs) (Hong Kong) Study (n = 4000) and the Taiwan Longitudinal Study on Aging (n = 2392). Frailty was defined as an index calculated from 30 multiple deficits. The ratio of the frailty index to life expectancy at birth (FI/LE) was used as an indicator of compression of morbidity. Frailty was more prevalent in Taiwan-urban (33.1%) and Taiwan-rural (38.1%) compared to Hong Kong (16.6%, p < 0.05) and was higher in women (22.6–49.7%) than in men (10.5–27.5%, p < 0.05). The ratios of FI/LE were higher in Taiwan-urban and Taiwan-rural (both 0.27) compared to Hong Kong (0.20, p < 0.05). Multivariate analyses revealed that older age, being a woman and low levels of physical activity were common risk factors for frailty across the three populations. Alcohol use was inversely associated with frailty in both Hong Kong and Taiwan-urban populations, but not in Taiwan-rural. Living alone was associated with frailty in Hong Kong men, but not in Hong Kong women or Taiwanese people. For all study populations, older age and being a woman constituted the highest attributable factor. This comparison provides useful data to inform government policies.
Attitudes, Beliefs and Predictors of Male Circumcision Promotion among Medical University Students in a Traditionally Non-Circumcising Region
Authors: Ganczak Maria , Korzeń Marcin , Olszewski Maciej
Published: September 2017

Objective: To evaluate the beliefs of medical university students regarding male circumcision (MC), as well as attitudes and the predictors of its promotion in the case of adults at risk of HIV. Methods: A cross-sectional survey was conducted between 2013–2016 at the Medical University in Szczecin, Poland, among final year Polish/foreign students from Northern Europe, using a standardized questionnaire. Results: There were 539 participants, median age 25 years, 40.8% males, and 66.8% were Polish nationals. The MC rate was 16.7%. Regarding HIV/AIDS knowledge, 66.6% of the students scored more than 75%; and, 34.2% knew that MC reduces the risk of HIV infection. One in eleven respondents (9.1%) believed that circumcised men felt more intense sexual pleasure. More than half of the respondents (54.8%) declared that they would recommend MC to adult patients at risk for HIV. The belief that circumcised men felt more intense sexual pleasure, and knowledge on MC regarding HIV risk reduction was associated with greater odds of recommending adult MC (OR = 3.35 and OR = 2.13, respectively). Conclusions: Poor knowledge of its benefits and a low willingness to promote the procedure—strongly dependent on personal beliefs—suggest that medical students may need additional training to help them to discuss MC more openly with adult men at risk for HIV infection. Knowledge may be an effective tool when making decisions regarding MC promotion.
Current Status of Early Blight Resistance in Tomato: An Update
Authors: Adhikari Pragya , Oh Yeonyee , Panthee Dilip
Published: September 2017

Early blight (EB) is one of the dreadful diseases of tomato caused by several species of Alternaria including Alternaria linariae (which includes A. solani and A. tomatophila), as well as A. alternata. In some instances, annual economic yield losses due to EB have been estimated at 79%. Alternaria are known only to reproduce asexually, but a highly-virulent isolate has the potential to overcome existing resistance genes. Currently, cultural practices and fungicide applications are employed for the management of EB due to the lack of strong resistant cultivars. Resistance sources have been identified in wild species of tomato; some breeding lines and cultivars with moderate resistance have been developed through conventional breeding methods. Polygenic inheritance of EB resistance, insufficient resistance in cultivated species and the association of EB resistance with undesirable horticultural traits have thwarted the effective breeding of EB resistance in tomato. Several quantitative trait loci (QTL) conferring EB resistance have been detected in the populations derived from different wild species including Solanum habrochaites, Solanum arcanum and S. pimpinellifolium, but none of them could be used in EB resistance breeding due to low individual QTL effects. Pyramiding of those QTLs would provide strong resistance. More research is needed to identify additional sources of useful resistance, to incorporate resistant QTLs into breeding lines through marker-assisted selection (MAS) and to develop resistant cultivars with desirable horticultural traits including high yielding potential and early maturity. This paper will review the current understanding of causal agents of EB of tomato, resistance genetics and breeding, problems associated with breeding and future prospects.
The Innate and Adaptive Immune System as Targets for Biologic Therapies in Inflammatory Bowel Disease
Authors: Holleran Grainne , Lopetuso Loris , Petito Valentina , Graziani Cristina , Ianiro Gianluca , McNamara Deirdre , Gasbarrini Antonio , Scaldaferri Franco
Published: September 2017

Inflammatory bowel disease (IBD) is an immune-mediated inflammatory condition causing inflammation of gastrointestinal and systemic cells, with an increasing prevalence worldwide. Many factors are known to trigger and maintain inflammation in IBD including the innate and adaptive immune systems, genetics, the gastrointestinal microbiome and several environmental factors. Our knowledge of the involvement of the immune system in the pathophysiology of IBD has advanced rapidly over the last two decades, leading to the development of several immune-targeted treatments with a biological source, known as biologic agents. The initial focus of these agents was directed against the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) leading to dramatic changes in the disease course for a proportion of patients with IBD. However, more recently, it has been shown that a significant proportion of patients do not respond to anti-TNF-α directed therapies, leading a shift to other inflammatory pathways and targets, including those of both the innate and adaptive immune systems, and targets linking both systems including anti-leukocyte trafficking agents-integrins and adhesion molecules. This review briefly describes the molecular basis of immune based gastrointestinal inflammation in IBD, and then describes how several current and future biologic agents work to manipulate these pathways, and their clinical success to date.
Salmonella O48 Serum Resistance is Connected with the Elongation of the Lipopolysaccharide O-Antigen Containing Sialic Acid
Authors: Pawlak Aleksandra , Rybka Jacek , Dudek Bartłomiej , Krzyżewska Eva , Rybka Wojciech , Kędziora Anna , Klausa Elżbieta , Bugla-Płoskońska Gabriela
Published: September 2017

Complement is one of the most important parts of the innate immune system. Some bacteria can gain resistance against the bactericidal action of complement by decorating their outer cell surface with lipopolysaccharides (LPSs) containing a very long O-antigen or with specific outer membrane proteins. Additionally, the presence of sialic acid in the LPS molecules can provide a level of protection for bacteria, likening them to human cells, a phenomenon known as molecular mimicry. Salmonella O48, which contains sialic acid in the O-antigen, is the major cause of reptile-associated salmonellosis, a worldwide public health problem. In this study, we tested the effect of prolonged exposure to human serum on strains from Salmonella serogroup O48, specifically on the O-antigen length. After multiple passages in serum, three out of four tested strains became resistant to serum action. The gas-liquid chromatography/tandem mass spectrometry analysis showed that, for most of the strains, the average length of the LPS O-antigen increased. Thus, we have discovered a link between the resistance of bacterial cells to serum and the elongation of the LPS O-antigen.
Cultivar and Metal-Specific Effects of Endophytic Bacteria in Helianthus tuberosus Exposed to Cd and Zn
Authors: Montalbán Blanca , Thijs Sofie , Lobo Mª Carmen , Weyens Nele , Ameloot Marcel , Vangronsveld Jaco , Pérez-Sanz Araceli
Published: September 2017

Plant growth promoting endophytic bacteria (PGPB) isolated from Brassica napus were inoculated in two cultivars of Helianthus tuberosus (VR and D19) growing on sand supplemented with 0.1 mM Cd or 1 mM Zn. Plant growth, concentrations of metals and thiobarbituric acid (TBA) reactive compounds were determined. Colonization of roots of H. tuberosus D19 by Pseudomonas sp. 262 was evaluated using confocal laser scanning microscopy. Pseudomonas sp. 228, Serratia sp. 246 and Pseudomonas sp. 262 significantly enhanced growth of H. tuberosus D19 exposed to Cd or Zn. Pseudomonas sp. 228 significantly increased Cd concentrations in roots. Serratia sp. 246, and Pseudomonas sp. 256 and 228 resulted in significantly decreased contents of TBA reactive compounds in roots of Zn exposed D19 plants. Growth improvement and decrease of metal-induced stress were more pronounced in D19 than in VR. Pseudomonas sp. 262-green fluorescent protein (GFP) colonized the root epidermis/exodermis and also inside root hairs, indicating that an endophytic interaction was established. H. tuberosus D19 inoculated with Pseudomonas sp. 228, Serratia sp. 246 and Pseudomonas sp. 262 holds promise for sustainable biomass production in combination with phytoremediation on Cd and Zn contaminated soils.
Sphingosine 1-Phosphate Signaling and Its Pharmacological Modulation in Allogeneic Hematopoietic Stem Cell Transplantation
Authors: Smith Philip , O’Sullivan Catherine , Gergely Peter
Published: September 2017

Allogeneic haemopoietic stem cell transplantation (HSCT) is increasingly used to treat haematological malignant diseases via the graft-versus-leukaemia (GvL) or graft-versus-tumour effects. Although improvements in infectious disease prophylaxis, immunosuppressive treatments, supportive care, and molecular based tissue typing have contributed to enhanced outcomes, acute graft-versus-host disease and other transplant related complications still contribute to high mortality and significantly limit the more widespread use of HSCT. Sphingosine 1-phosphate (S1P) is a zwitterionic lysophospholipid that has been implicated as a crucial signaling regulator in many physiological and pathophysiological processes including multiple cell types such as macrophages, dendritic cells, T cells, T regulatory cells and endothelial cells. Recent data suggested important roles for S1P signaling in engraftment, graft-versus-host disease (GvHD), GvL and other processes that occur during and after HSCT. Based on such data, pharmacological intervention via S1P modulation may have the potential to improve patient outcome by regulating GvHD and enhancing engraftment while permitting effective GvL.
Comparison of T-Norms and S-Norms for Interval Type-2 Fuzzy Numbers in Weight Adjustment for Neural Networks
Authors: Gaxiola Fernando , Melin Patricia , Valdez Fevrier , Castillo Oscar , Castro Juan
Published: September 2017

A comparison of different T-norms and S-norms for interval type-2 fuzzy number weights is proposed in this work. The interval type-2 fuzzy number weights are used in a neural network with an interval backpropagation learning enhanced method for weight adjustment. Results of experiments and a comparative research between traditional neural networks and the neural network with interval type-2 fuzzy number weights with different T-norms and S-norms are presented to demonstrate the benefits of the proposed approach. In this research, the definitions of the lower and upper interval type-2 fuzzy numbers with random initial values are presented; this interval represents the footprint of uncertainty (FOU). The proposed work is based on recent works that have considered the adaptation of weights using type-2 fuzzy numbers. To confirm the efficiency of the proposed method, a case of data prediction is applied, in particular for the Mackey-Glass time series (for τ = 17). Noise of Gaussian type was applied to the testing data of the Mackey-Glass time series to demonstrate that the neural network using a interval type-2 fuzzy numbers method achieves a lower susceptibility to noise than other methods.
Biomineralization of Fucoidan-Peptide Blends and Their Potential Applications in Bone Tissue Regeneration
Authors: Pajovich Harrison , Banerjee Ipsita
Published: September 2017

Fucoidan (Fuc), a natural polysaccharide derived from brown seaweed algae, and gelatin (Gel) were conjugated to form a template for preparation of biomimetic scaffolds for potential applications in bone tissue regeneration. To the Fuc–Gel we then incorporated the peptide sequence MTNYDEAAMAIASLN (MTN) derived from the E-F hand domain, known for its calcium binding properties. To mimic the components of the extracellular matrix of bone tissue, the Fuc–Gel–MTN assemblies were incubated in simulated body fluid (SBF) to induce biomineralization, resulting in the formation of β-tricalcium phosphate, and hydroxyapatite (HAp). The formed Fuc–Gel–MTN–beta–TCP/HAP scaffolds were found to display an average Young’s Modulus value of 0.32 GPa (n = 5) with an average surface roughness of 91 nm. Rheological studies show that the biomineralized scaffold exhibited higher storage and loss modulus compared to the composites formed before biomineralization. Thermal phase changes were studied through DSC and TGA analysis. XRD and EDS analyses indicated a biphasic mixture of β-tricalcium phosphate and hydroxyapatite and the composition of the scaffold. The scaffold promoted cell proliferation, differentiation and displayed actin stress fibers indicating the formation of cell-scaffold matrices in the presence of MT3C3-E1 mouse preosteoblasts. Osteogenesis and mineralization were found to increase with Fuc–Gel–MTN–beta–TCP/HAP scaffolds. Thus, we have developed a novel scaffold for possible applications in bone tissue engineering.
Facile Synthesis of ZnO Nanoparticles on Nitrogen-Doped Carbon Nanotubes as High-Performance Anode Material for Lithium-Ion Batteries
Authors: Li Haipeng , Liu Zhengjun , Yang Shuang , Zhao Yan , Feng Yuting , Bakenov Zhumabay , Zhang Chengwei , Yin Fuxing
Published: September 2017

ZnO/nitrogen-doped carbon nanotube (ZnO/NCNT) composite, prepared though a simple one-step sol-gel synthetic technique, has been explored for the first time as an anode material. The as-prepared ZnO/NCNT nanocomposite preserves a good dispersity and homogeneity of the ZnO nanoparticles (~6 nm) which deposited on the surface of NCNT. Transmission electron microscopy (TEM) reveals the formation of ZnO nanoparticles with an average size of 6 nm homogeneously deposited on the surface of NCNT. ZnO/NCNT composite, when evaluated as an anode for lithium-ion batteries (LIBs), exhibits remarkably enhanced cycling ability and rate capability compared with the ZnO/CNT counterpart. A relatively large reversible capacity of 1013 mAh·g−1 is manifested at the second cycle and a capacity of 664 mAh·g−1 is retained after 100 cycles. Furthermore, the ZnO/NCNT system displays a reversible capacity of 308 mAh·g−1 even at a high current density of 1600 mA·g−1. These electrochemical performance enhancements are ascribed to the reinforced accumulative effects of the well-dispersed ZnO nanoparticles and doping nitrogen atoms, which can not only suppress the volumetric expansion of ZnO nanoparticles during the cycling performance but also provide a highly conductive NCNT network for ZnO anode.
Novel Self-Assembly-Induced Gelation for Nanofibrous Collagen/Hydroxyapatite Composite Microspheres
Authors: Choi Jae-Won , Kim Jong-Woo , Jo In-Hwan , Koh Young-Hag , Kim Hyoun-Ee
Published: September 2017

This study demonstrates the utility of the newly developed self-assembly-induced gelation technique for the synthesis of porous collagen/hydroxyapatite (HA) composite microspheres with a nanofibrous structure. This new approach can produce microspheres of a uniform size using the droplets that form at the nozzle tip before gelation. These microspheres can have a highly nanofibrous structure due to the immersion of the droplets in a coagulation bath (water/acetone), in which the collagen aggregates in the solution can self-assemble into fibrils due to pH-dependent precipitation. Bioactive HA particles were incorporated into the collagen solutions, in order to enhance the bioactivity of the composite microspheres. The composite microspheres exhibited a well-defined spherical morphology and a uniform size for all levels of HA content (0 wt %, 10 wt %, 15 wt %, and 20 wt %). Collagen nanofibers—several tens of nanometers in size—were uniformly present throughout the microspheres and the HA particles were also well dispersed. The in vitro apatite-forming ability, assessed using the simulated body fluid (SBF) solution, increased significantly with the incorporation of HA into the composite microspheres.
The Application of Functionalized Pillared Porous Phosphate Heterostructures for the Removal of Textile Dyes from Wastewater
Authors: Jiménez-Jiménez José , Algarra Manuel , Guimarães Vanessa , Bobos Iuliu , Rodríguez-Castellón Enrique
Published: September 2017

A synthesized functionalized pillared porous phosphate heterostructure (PPH), surface functionalized phenyl group, has been used to remove the dye Acid Blue 113 from wastewater. X-ray photoemission spectroscopy XPS and X-ray diffraction (XRD) were used to study its structure. The specific surface area of this was 498 m2/g. The adsorption capacities of PPH and phenyl surface functionalized (Φ-PPH) were 0.0400 and 0.0967 mmol/g, respectively, with a dye concentration of 10−5 M when well fitted with SIPS and Langmuir isotherms respectively (pH 6.5, 25 °C). The incorporation of the dye to the adsorbent material was monitored by the S content of the dye. It is suggested as an alternative for Acid Blue 113 remediation.
Milling Positive Master for Polydimethylsiloxane Microfluidic Devices: The Microfabrication and Roughness Issues
Authors: Zhou Zhizhi , Chen Dong , Wang Xiang , Jiang Jiahuan
Published: September 2017

We provide a facile and low-cost method (F-L) to fabricate a two-dimensional positive master using a milling technique for polydimethylsiloxane (PDMS)-based microchannel molding. This method comprises the following steps: (1) a positive microscale master of the geometry is milled on to an acrylic block; (2) pre-cured PDMS is used to mold the microscale positive master; (3) the PDMS plate is peeled off from the master and punctured with a blunt needle; and (4) the PDMS plate is O2 plasma bonded to a glass slide. Using this technique, we can fabricate microchannels with very simple protocols quickly and inexpensively. This method also avoids breakage of the end mill (ϕ = 0.4 mm) of the computerized numerical control (CNC) system when fabricating the narrow channels (width < 50 µm). The prominent surface roughness of the milled bottom-layer could be overcomed by pre-cured PDMS with size trade-off in design. Finally, emulsion formation successfully demonstrates the validity of the proposed fabrication protocol. This work represents an important step toward the use of a milling technique for PDMS-based microfabrication.
Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells
Authors: Lee Seulah , Lee Dahae , Jang Tae , Kang Ki , Nam Joo-Won , Lee Hae-Jeung , Kim Ki
Published: September 2017

The edible fungus Phellinus baumii Pilat (Hymenochaetaceae) has been used in Korean traditional medicines for strengthening health and prolonging life. An extract of the fruiting bodies of P. baumii was subjected to bioassay-guided fractionation based on its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The resulting fractions were chemically investigated, leading to isolation of three phenolic compounds (1–3), a sesquiterpene (4), two steroids (5–6), a fatty acid (7), and a cerebroside (8). Spectroscopic analyses including 1D and 2D NMR spectroscopy and LC/MS were used to determine their chemical structures. Compounds 2, 4, 5, 7 and 8 were identified in P. baumii for the first time. Since all compounds were isolated from active fractions with anti-inflammatory activity, their ability to inhibit LPS-stimulated nitric oxide (NO) production in RAW264.7 cells were evaluated in vitro. Compounds 1, 2, 3, 5 and 7 inhibited LPS-stimulated NO production, and compounds 1–3 had IC50 values <10 μM. Treatment of LPS-stimulated RAW264.7 cells with compounds 1–3 inhibited phosphorylation of IKKα and IκBα. In addition, treatment of compounds 1–3 reduced LPS-induced increases of nuclear factor-kappa B (NF-κB) p65, iNOS and COX-2 protein expressions. Collectively, compounds 1–3 inhibited NF-κB-dependent inflammation in RAW264.7 cells. Thus, P. baumii is a potential source of natural anti-inflammatory agents, and active compounds 1–3 could be promising lead compounds for the development of novel anti-inflammatory agents.
The Recognition of Calmodulin to the Target Sequence of Calcineurin—A Novel Binding Mode
Authors: Chyan Chia-Lin , Irene Deli , Lin Sin-Mao
Published: September 2017

Calcineurin (CaN) is a Ca2+/calmodulin-dependent Ser/Thr protein phosphatase, which plays essential roles in many cellular and developmental processes. CaN comprises two subunits, a catalytic subunit (CaN-A, 60 kDa) and a regulatory subunit (CaN-B, 19 kDa). CaN-A tightly binds to CaN-B in the presence of minimal levels of Ca2+, but the enzyme is inactive until activated by CaM. Upon binding to CaM, CaN then undergoes a conformational rearrangement, the auto inhibitory domain is displaced and thus allows for full activity. In order to elucidate the regulatory role of CaM in the activation processes of CaN, we used NMR spectroscopy to determine the structure of the complex of CaM and the target peptide of CaN (CaNp). The CaM/CaNp complex shows a compact ellipsoidal shape with 8 α-helices of CaM wrapping around the CaNp helix. The RMSD of backbone and heavy atoms of twenty lowest energy structures of CaM/CaNp complex are 0.66 and 1.14 Å, respectively. The structure of CaM/CaNp complex can be classified as a novel binding mode family 1–18 with major anchor residues Ile396 and Leu413 to allocate the largest space between two domains of CaM. The relative orientation of CaNp to CaM is similar to the CaMKK peptide in the 1–16 binding mode with N- and C-terminal hydrophobic anchors of target sequence engulfed in the hydrophobic pockets of the N- and C-domain of CaM, respectively. In the light of the structural model of CaM/CaNp complex reported here, we provide new insight in the activation processes of CaN by CaM. We propose that the hydrophobic interactions between the Ca2+-saturated C-domain and C-terminal half of the target sequence provide driving forces for the initial recognition. Subsequent folding in the target sequence and structural readjustments in CaM enhance the formation of the complex and affinity to calcium. The electrostatic repulsion between CaM/CaNp complex and AID may result in the displacement of AID from active site for full activity.
Antimicrobial Nanomaterials: Why Evolution Matters
Authors: Graves Joseph , Thomas Misty , Ewunkem Jude
Published: September 2017

Due to the widespread occurrence of multidrug resistant microbes there is increasing interest in the use of novel nanostructured materials as antimicrobials. Specifically, metallic nanoparticles such as silver, copper, and gold have been deployed due to the multiple impacts they have on bacterial physiology. From this, many have concluded that such nanomaterials represent steep obstacles against the evolution of resistance. However, we have already shown that this view is fallacious. For this reason, the significance of our initial experiments are beginning to be recognized in the antimicrobial effects of nanomaterials literature. This recognition is not yet fully understood and here we further explain why nanomaterials research requires a more nuanced understanding of core microbial evolution principles.
Phyllodulcin, a Natural Sweetener, Regulates Obesity-Related Metabolic Changes and Fat Browning-Related Genes of Subcutaneous White Adipose Tissue in High-Fat Diet-Induced Obese Mice
Authors: Kim Eunju , Lim Soo-Min , Kim Min-Soo , Yoo Sang-Ho , Kim Yuri
Published: September 2017

Phyllodulcin is a natural sweetener found in Hydrangea macrophylla var. thunbergii. This study investigated whether phyllodulcin could improve metabolic abnormalities in high-fat diet (HFD)-induced obese mice. Animals were fed a 60% HFD for 6 weeks to induce obesity, followed by 7 weeks of supplementation with phyllodulcin (20 or 40 mg/kg body weight (b.w.)/day). Stevioside (40 mg/kg b.w./day) was used as a positive control. Phyllodulcin supplementation reduced subcutaneous fat mass, levels of plasma lipids, triglycerides, total cholesterol, and low-density lipoprotein cholesterol and improved the levels of leptin, adiponectin, and fasting blood glucose. In subcutaneous fat tissues, supplementation with stevioside or phyllodulcin significantly decreased mRNA expression of lipogenesis-related genes, including CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferator activated receptor γ (PPARγ), and sterol regulatory element-binding protein-1C (SREBP-1c) compared to the high-fat group. Phyllodulcin supplementation significantly increased the expression of fat browning-related genes, including PR domain containing 16 (Prdm16), uncoupling protein 1 (UCP1), and peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α), compared to the high-fat group. Hypothalamic brain-derived neurotrophic factor-tropomyosin receptor kinase B (BDNF-TrkB) signaling was upregulated by phyllodulcin supplementation. In conclusion, phyllodulcin is a potential sweetener that could be used to combat obesity by regulating levels of leptin, fat browning-related genes, and hypothalamic BDNF-TrkB signaling.
Intestinal Production of Anti-Tissue Transglutaminase 2 Antibodies in Patients with Diagnosis Other Than Celiac Disease
Authors: Maglio Mariantonia , Ziberna Fabiana , Aitoro Rosita , Discepolo Valentina , Lania Giuliana , Bassi Virginia , Miele Erasmo , Not Tarcisio , Troncone Riccardo , Auricchio Renata
Published: September 2017

It has been hypothesized that gluten-dependent production of anti-tissue-transglutaminase 2 (anti-TG2) antibodies may occur only at an intestinal level. We have investigated intestinal production of anti-TG2 antibodies in 136 patients with normal serum levels of anti-TG2 antibodies and normal duodenal mucosa. Intestinal deposits of anti-TG2 antibodies were evaluated by immunofluorescence and anti-TG2 antibodies released in organ culture supernatants measured by ELISA. Intestinal antibody libraries were obtained from 10 subjects. Immunohistochemistry for CD25+, CD3+, and TCR-γδ+ was assessed in subjects with positive (n = 32) and negative (n = 31) intestinal anti-TG2 antibodies. Globally 33/136 (24%) seronegative patients produced anti-TG2 autoantibodies at an intestinal level. Antibody libraries analysis confirmed the anti-TG2 antibodies mucosal production in all (n = 8) positive subjects. Lamina propria CD25+ cell count was significantly (p < 0.05) higher in patients with intestinal anti-TG2. Moreover, 13/32 (41%) of them showed high TCR-γδ+/CD3+ ratios. Intestinal anti-TG2 antibody production does not show absolute specificity for CD. It is seen more often in association with inflamed mucosa. Further investigations are necessary to prove the possible role of dietary gluten.
Inhibitory Effects of Culinary Herbs and Spices on the Growth of HCA-7 Colorectal Cancer Cells and Their COX-2 Expression
Authors: Jaksevicius Andrius , Carew Mark , Mistry Calli , Modjtahedi Helmout , Opara Elizabeth
Published: September 2017

It is unclear if the anti-inflammatory properties of culinary herbs and spices (CHS) are linked to their ability to inhibit Colorectal cancer cell (CRC) growth. Furthermore, their therapeutic potential with regards to CRC is unknown. The aim of this study was to establish if the inhibition of HCA-7 CRC cell growth by a selection of culinary herbs and spices (CHS) is linked to the inhibition of the cells’ cyclooxygenase-2 (COX-2 )expression, and to investigate their therapeutic potential. CHS inhibited the growth of Human colon adenocarcinoma-7 (HCA-7) cells; the order of potency was turmeric, bay leaf, ginger, sage, and rosemary; their combinations had a synergistic or additive effect on cell growth inhibition. CHS also inhibited COX-2 expression and activity; this action was comparable to that of the specific COX-2 inhibitor Celecoxib. Coincident with COX-2 inhibition was the accumulation of cells in the sub G1 phase of the HCA-7’s cell cycle and, using bay leaf and turmeric, the cleavage of caspase 3 and poly (ADP-ribose) polymerase (PARP). This latter effect showed that the effect of these CHS on growth arrest was irreversible, and was comparable to that of the caspase activator Etoposide. This study provides evidence of a link between the inhibition of HCA-7 growth, and its COX-2 expression, by CHS, and their therapeutic potential.
Topical Nano and Microemulsions for Skin Delivery
Authors: Nastiti Christofori , Ponto Thellie , Abd Eman , Grice Jeffrey , Benson Heather , Roberts Michael
Published: September 2017

Nanosystems such as microemulsions (ME) and nanoemulsions (NE) offer considerable opportunities for targeted drug delivery to and via the skin. ME and NE are stable colloidal systems composed of oil and water, stabilised by a mixture of surfactants and cosurfactants, that have received particular interest as topical skin delivery systems. There is considerable scope to manipulate the formulation components and characteristics to achieve optimal bioavailability and minimal skin irritancy. This includes the incorporation of established chemical penetration enhancers to fluidize the stratum corneum lipid bilayers, thus reducing the primary skin barrier and increasing permeation. This review discusses nanosystems with utility in skin delivery and focuses on the composition and characterization of ME and NE for topical and transdermal delivery. The mechanism of skin delivery across the stratum corneum and via hair follicles is reviewed with particular focus on the influence of formulation.
Fabrication of Electrospun Polylactic Acid/Cinnamaldehyde/β-Cyclodextrin Fibers as an Antimicrobial Wound Dressing
Authors: Liu Yaowen , Liang Xue , Zhang Rong , Lan Wenting , Qin Wen
Published: September 2017

Cinnamaldehyde (CA) was successfully encapsulated in β-cyclodextrin (β-CD), and polylactic acid (PLA)-based composite fibers were prepared by incorporating CA/β-CD via electrospinning. Morphological, structural, spectral, and antibacterial properties of different weight ratios of PLA:β-CD/CA (88:12, 94:6, 97:3, and 98.5:1.5) and PLA/CA/β-CD fibers were investigated. PLA and CA/β-CD were incorporated by mixing of CA/β-CD inclusions to enhance the viscosity of the mixed solution. The mechanical properties and hydrophilicity of nanofibers were improved following the addition of CA/β-CD. Moreover, CA/β-CD improved the antibacterial activities of the mixture against Escherichia coli and Staphylococcus aureus. PLA/CA/β-CD-3 exhibited excellent antibacterial effects and low cytotoxicity. Thus, our study showed that PLA/CA/β-CD fibers may have applications as wound dressing materials and for use in other biomedical applications.
The Impact of Risk Management in Credit Rating Agencies
Authors: Seetharaman A. , Kumar Sahu Vikas , Saravanan A. , Rudolph Raj John , Niranjan Indu
Published: September 2017

An empirical study was conducted to determine the impact of different types of risk on the performance management of credit rating agencies (CRAs). The different types of risks were classified as operational, market, business, financial, and credit. All these five variables were analysed to ascertain their impact on the performance of CRAs. In addition, apart from identifying the significant variables, the study focused on setting out a structured framework for future research. The five independent variables were tested statistically using structural equation modelling (SEM). The results indicated that market risk, financial risk, and credit risk have a significant impact on the performance of CRAs, whereas operational risk and business risk, though important, do not have a significant influence. This finding has a significant implication for the examination and inter-firm evaluation of CRAs.
Examining the Influence of Crop Residue Burning on Local PM2.5 Concentrations in Heilongjiang Province Using Ground Observation and Remote Sensing Data
Authors: Chen Ziyue , Chen Danlu , Zhuang Yan , Cai Jun , Zhao Na , He Bin , Gao Bingbo , Xu Bing
Published: September 2017

Although a many studies concerning crop residue burning have been conducted, the influence of crop residue burning on local PM2.5 concentrations remains unclear. The number of crop residue burning spots was the highest in Heilongjiang province and we extracted crop residue burning spots for this region using MOD14A1 (Thermal Anomalies & Fire Daily L3 Global 1 km) data and national land cover data. By analyzing the temporal variation of crop residue burning and PM2.5 concentrations in Heilongjiang province, we found that the total number of crop residue burning spots was not correlated with the variations of PM2.5 concentrations at a provincial (regional) scale. However, crop residue burning exerted notable influence on the variations of PM2.5 concentrations at a local scale. We experimented with a set of buffer zone radiuses to examine the influencing area of crop residue burning. The results suggest that the valid influencing area of crop residue burning was between 50 and 80 km. The mean PM2.5 concentration measured at stations close to crop residue burning spots was more than 60 μg/m3 higher than that measured at stations not close to crop residue burning spots. However, no consistent, significant correlation existed between the existence of crop residue burning spots and local PM2.5 concentrations, indicating that local PM2.5 concentrations were influenced by a diversity of factors and not solely controlled by crop residue burning. This research also provides suggestions for better understanding the role of crop residue burning in local and regional air pollution.
Pose Estimation of a Mobile Robot Based on Fusion of IMU Data and Vision Data Using an Extended Kalman Filter
Authors: Alatise Mary , Hancke Gerhard
Published: September 2017

Using a single sensor to determine the pose estimation of a device cannot give accurate results. This paper presents a fusion of an inertial sensor of six degrees of freedom (6-DoF) which comprises the 3-axis of an accelerometer and the 3-axis of a gyroscope, and a vision to determine a low-cost and accurate position for an autonomous mobile robot. For vision, a monocular vision-based object detection algorithm speeded-up robust feature (SURF) and random sample consensus (RANSAC) algorithms were integrated and used to recognize a sample object in several images taken. As against the conventional method that depend on point-tracking, RANSAC uses an iterative method to estimate the parameters of a mathematical model from a set of captured data which contains outliers. With SURF and RANSAC, improved accuracy is certain; this is because of their ability to find interest points (features) under different viewing conditions using a Hessain matrix. This approach is proposed because of its simple implementation, low cost, and improved accuracy. With an extended Kalman filter (EKF), data from inertial sensors and a camera were fused to estimate the position and orientation of the mobile robot. All these sensors were mounted on the mobile robot to obtain an accurate localization. An indoor experiment was carried out to validate and evaluate the performance. Experimental results show that the proposed method is fast in computation, reliable and robust, and can be considered for practical applications. The performance of the experiments was verified by the ground truth data and root mean square errors (RMSEs).
LEED, Its Efficacy and Fallacy in a Regional Context—An Urban Heat Island Case in California
Authors: Shin Min , Kim Hwan , Gu Donghwan , Kim Hyoungsub
Published: September 2017

The use of energy in the building sector has increased rapidly over the past two decades. Accordingly, various building assessment methods have developed in green building practices. However, the questions still remain in regard to how positively green buildings affect regional surroundings. This study investigates the possible relationship between LEED-certified buildings and urban heat island effect. Using GIS with spatial regression, the study found that constructing an LEED building in a 30-m boundary could possibly lower the temperature of the surrounding environment by 0.35 °C. Also, having a higher certification level, such as Gold or Platinum, increased the lowering effect by 0.48 °C, while a lower certification level, such as Certified or Silver, had a lowering effect of 0.26 °C. Although LEED has gained a substantial amount of interest and skepticism at the same time, the study results could be a potential sign that the Sustainable Sites Credits or energy-efficient materials play a positive role in lowering the temperature.
Development of an Evapotranspiration Data Assimilation Technique for Streamflow Estimates: A Case Study in a Semi-Arid Region
Authors: Zhang Ying , Zhang Ling , Hou Jinliang , Gu Juan , Huang Chunlin
Published: September 2017

Streamflow estimates are substantially important as fresh water shortages increase in arid and semi-arid regions where evapotranspiration (ET) is a significant contribution to the water balance. In this regard, evapotranspiration data can be assimilated into a distributed hydrological model (SWAT, Soil and Water Assessment Tool) for improving streamflow estimates. The SWAT model has been widely used for streamflow estimations, but the applications combining SWAT and ET products were rare. Thus, this study aims to develop a SWAT-based evapotranspiration data assimilation system. In particular, SWAT is gridded at Hydrologic Response Unit (HRU) level to incorporate gridded ET products acquired from the remote sensing-based ETMonitor model. In the modeling case, Gridded SWAT (GSWAT) shows a good agreement of streamflow modeling with the original SWAT. Such a scant margin between them is due to the modeling domain mismatch caused by different HRU delineations. In the ET assimilation case, we carry out a synthetic data experiment to illustrate the state augmentation Direct Insertion (DI) method and a real data experiment for the upper Heihe River Basin. The results demonstrate the benefits of the ET assimilation for improving hydrologic processes representations. In the future, more remotely sensed data can be assimilated into the data assimilation system to provide more reliable hydrological predictions.
Stability Control of Retained Goaf-Side Gateroad under Different Roof Conditions in Deep Underground Y Type Longwall Mining
Authors: Zhang Zhiyi , Shimada Hideki , Sasaoka Takashi , Hamanaka Akihiro
Published: September 2017

Stability of the retained goaf-side gateroad (RGSG) is influenced mainly by the movements of the roof strata near coal seam after coalface passes by. To make effective controlling technology for the stability of the RGSG, we analyze the roof structure over the RGSG to illustrate the mechanism causing the RGSG instability under different roof conditions. We then examine the dynamic evolution of the deformation and abutment stress in the rock surrounding the RGSG during coal seam mining, using the FLAC3D numerical software to reveal the instability characteristics of the RGSG under different roof conditions. Next, corresponding stability controlling technologies for the RGSGs are proposed and tested in three typical deep underground coalmines. Results show that: sink and rotation of the roof cantilever over the RGSG impose severer influence on the stability of the RGSG. The RGSG suffers disturbances three times during the coal-seam mining, and the deformation and abutment stress in the rock surrounding the RGSG increase significantly when the main roof becomes thicker and the immediate roof becomes thinner. Staged support technology involving grout cable bolts has better controlling results of the RGSG stability than that composed of conventional rock bolts, when the RGSG is beneath weak immediate roof with large thickness. Roof structure optimizing technology involving pre-split technology can improve the stability of the RGSG effectively when the RGSG is covered by hard main roof with large thickness directly.
Three-Fold Embedding: Farm Development in Relation to Its Socio-Material Context
Authors: Methorst Ron , Roep Dirk , Verstegen Jos , Wiskerke Johannes
Published: September 2017

Understanding heterogeneity in farm development strategies is important in order to design effective policies and support programs to increase the sustainability of agriculture in relation to its socio-material context. Using a unique case study of 102 dairy farmers in The Netherlands, all operating in a highly similar socio-material context, this paper studies the differences in the relation of farms with the socio-material context. To this end, the concept of three-fold embedding is developed and tested using the ideal-typical characteristics of three patterns of farm development that were identified in the case study: (1) maximising total milk production; (2) optimising milk production using mainly on-farm resources; and (3) diversified on-farm production. Three-fold embedding was conceptualised in relation to the following dimensions: (1) value chain relations; (2) socio-cultural relations; and (3) natural resource relations, while using a scale ranging from a Close to a Stretched set of relations. The concept of three-fold embedding proves to be useful to identify in a non-normative and non-binary manner meaningful differences between patterns of farm development. These differences relate to differences in opportunity identification and strategic decision-making. The concept supports a relational perspective in studying farm strategy development using a sociology of entrepreneurship approach.