bluuebunny/crossref_metadata_2025 huggingface dataset:
Snap-Frozen Tissue Preparation v1
Authors: Fisher Stephen
Published: October 2022

This protocol describes preservation of tissue by snap-freezing. Biospecimens preserved with this protocol will be suitable for downstream analysis of DNA, RNA, protein, and morphology endpoints. Multiple workflows can be used for snap freezing including using placing tissue in a vessel either with or without media and exposing it to liquid nitrogen vapor, immersing it in liquid nitrogen, or placing it on dry ice with a cooling device (Micke et al., 2006). The workflow pursued depends on the desired balance between feasibility, time, and cost as well as the possible downstream analyses performed. The snap-freezing protocol detailed below may not be ideal for downstream assays that require intact tissue morphology (Engel, Vaught, & Moore, 2014) (see NCI Biospecimen Evidence-Based Practices). Micke, P., Ohshima, M., Tahmasebpoor, S., Ren, Z.-P., Ostman, A., Pontén, F., & Botling, J. (2006). Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens.Laboratory Investigation; a Journal of Technical Methods and Pathology,86(2), 202–211. https://doi.org/10.1038/labinvest.3700372 Engel, K. B., Vaught, J., & Moore, H. M. (2014). National Cancer Institute Biospecimen Evidence-Based Practices: A novel approach to pre-analytical standardization.Biopreservation and Biobanking,12(2), 148–150. https://doi.org/10.1089/bio.2013.0091
PCR normalization and size selection with magnetic beads v1
Authors: Buchner Dominik
Published: October 2022

This protocol describes how to clean up and normalize PCR products or DNA extracts and perform a size selection with carboxylated-magnetic beads and a PEG-NaCl buffer. It works by diluting the beads so that the binding capacity is lower than the PCR yield which leads to a normalization of all PCR products to the binding capacity.
Long-term analysis of сanagliflozin budget impact in adult patients with type 2 diabetes mellitus and diabetic nephropathy in the Russian Federation
Authors: Avxentyev N. A. , Makarova Yu. V.
Published: October 2022

Background. Diabetic nephropathy (DN) is a specific kidney lesion in patients with diabetes mellitus, which leads to the development of endstage kidney disease and requires substitutive renal therapy (dialysis or transplantation). Canagliflozin is a sodium-glucose co-transporter 2 (SGLT2) inhibitor, which exerts a renoprotective effect. According to the published data, the application of canagliflozin in patients with type 2 diabetes mellitus (DM2) and DN could postpone dialysis therapy for almost 13 years.

Objective: to perform a long-term analysis of canagliflozin budget impact in adult patients with DM2 and DN from the point of view of the constituent entities of the Russian Federation.

Material and methods. A group of comparison for canagliflozin was placebo (no renoprotective pharmacotherapy). The authors proposed a mathematical model for DN progression in groups of patients who received canagliflozin (100 mg orally, daily, long-term) or placebo. The model was based on the extrapolation of the CREDENCE study data. The model was used for the analysis of the direct costs on the lifetime pharmacotherapy and dialysis per one patient. Epidemiologic data from the federal register on the number of adult patients with DM and DN prevalence among these patients were used to evaluate the size of the target population. Based on the clinical recommendations and the medical care standards for patients with DM and the data on the actual state procurement of SGLT2 inhibitors and glucagon-like peptide-1 receptor agonists in 2021, we evaluated the share of patients with DM2+DN who currently do not receive renoprotective therapy but who must be supplied with canagliflozin according to the standards. Among this population, we determined the patients with the glomerular filtration rate within 30–90 ml/min/1.73 m2, which provides the comparability of the target population with patients included in CREDENCE clinical study.

Results. Direct medical costs per one patient receiving canagliflozin therapy were 678 108 rubles, which was 52.8% (759,239 rubles) lower than without renoprotective pharmacotherapy (1,437,347 rubles). As a result, considering the modeling period and current practice, the budget costs for pharmacotherapy of patients with DM2+DN were 99.82 billion rubles, in comparison with the proposed practice, which was 47.09 billion rubles (difference in budget costs is 52.73 billion rubles, or 52.8%). The accumulated costs of the regional health care system were lower in patients receiving canagliflozin in comparison with patients without renoprotective pharmacotherapy 11 years after the beginning of treatment.

Conclusion. The expansion of canagliflozin application in the therapy for patients with DM2+DN leads to the budget cost cuts in the long run due to the extension of the dialysis-free period.
Mitigating Power and Memory Constraints on a Venusian Seismometer
Authors: Tian Yuan , Herrick Robert R. , West Michael E. , Kremic Tibor
Published: October 2022

The nearest term pathway to the deployment of a seismometer on Venus is an instrument that can operate under ambient surface conditions on battery power. We conduct a series of studies on combined hardware and software approaches to maximize the quality of data returned under the likely restrictions of minimal on-board data storage and only being able to transmit in real time during a small fraction of a multimonth deployment. We assess likely Venus seismicity by examining different terrestrial analog settings; we find that likely Venus analog settings all fall within about an order of magnitude of mean Earth in terms of seismicity level. We use the seismic record from a station in central Alaska as a Venus surrogate for algorithm development. We tested various transmission triggers and developed a simple low-memory algorithm that mimics the common terrestrial long-term average/short-term average trigger. If the seismometer can operate in coordination with an orbiter that can remotely turn off data transmission, then the frequency content of a few seconds of data can be used to distinguish small, nearby earthquakes from large, distal ones, and total data transmission can be tuned to favor the latter. If an orbiter can also turn on transmission for other nearby seismometers, it would further enhance the ability to distinguish small- and large-magnitude earthquakes autonomously and increase the chances of capturing the initial onset of significant events.
Deformation and quaternary faulting in southeast Missouri across the Commerce geophysical lineament
Authors: Stephenson W. J. , Odum J. K. , Williams R. A. , Pratt T. L. , Harrison R. W. , Hoffman D.
Published: October 2022

High-resolution seismic-reflection data acquired at three sites along the surface projection of the Commerce geophysical lineament in southeast Missouri reveal a complex history of post-Cretaceous faulting that has continued into the Quaternary. Near Qulin, Missouri, approximately 20 m of apparent vertical fault displacement has occurred in the Quaternary. Reflection data collected at Idalia Hill, about 45 km to the northeast, reveal a series of reverse and possibly right-lateral strike-slip faults with Quaternary displacement. In the Benton Hills, 45 km northeast of Idalia Hill, seismic data image a complicated series of anticlinal and synclinal fault-bounded blocks immediately north of the Commerce fault. We infer that most of the deformation imaged in the upper 400 m of these three data sets occurred since post-Cretaceous time, and a significant portion of it occurred during Quaternary time. Collectively, these seismic data along with geomorphic and surface-geologic evidence suggest (1) the existence of at least one potential seismogenic structure in southeastern Missouri outside the main zones of New Madrid seismicity, and (2) these structures have been active during the Quaternary. The geographic location of the imaged deformation suggests it is related to structures along with the Commerce geophysical lineament.
Deconvolution of teleseismic body waves for enhancing structure beneath a seismometer array
Authors: Li Xiao-Qing , Nábělek John L.
Published: October 2022

We discuss three stacking deconvolution techniques for enhancing signal of Earth structure contained in the seismograms of teleseismic body waves recorded by a seismometer array. The goal of the deconvolution is to remove from the seismograms the complications due to the source. For three-component data, the removal of the source can be efficiently achieved by deconvolving one component from another, as in the receiver function analysis. In the case of an array, if the structure beneath individual stations varies, the source wavelet can be estimated and removed from the seismograms by stacking. This allows structural analysis in the situations when only single-component seismometers are used or when a particular component can provide additional independent information. Here we compare the efficacy of conventional time-domain stacking, cepstral averaging, and autocorrelation stacking. The latter is a new approach developed here. For cepstral averaging, we present conditions that stabilize the convergence. Examples are presented using synthetics and real data from a broadband seismic array experiment conducted in the Cascadia subduction zone (Oregon) in 1993 to 1994. Data from subduction zones are particularly well suited because of the spatially rapidly varying structure. We find that all three methods work well for low frequencies, which are not sensitive to the time alignment of the seismic traces. The latter two methods do not require precisely aligned seismograms, and their advantage becomes evident at higher frequencies where the appropriate time alignment is harder to achieve. At high frequencies, time-domain stacking is likely to fail.
Outlier detection from a mixture distribution when training data are unlabeled
Authors: Sain Stephan R. , Gray H. L. , Woodward Wayne A. , Fisk Mark D.
Published: October 2022

We consider the difficult task of using seismic signals (or any other discriminants) for detecting nuclear explosions from the large number of background signals such as earthquakes and mining blasts. Given a ground-truth database (i.e., labeled data), Fisk et al. (1996) consider the problem of detecting outliers (nuclear explosions) from a single background-signal population, and their approach has been applied successfully in several regions around the world. Wang et al. (1997) attack the problem in terms of modeling the background as a mixture distribution and looking for outliers (nuclear events) from that mixture. However, those authors only considered the case in which at least some fraction of the training sample was labeled, that is, at least some ground-truth information was available, and the number of distinct classes of events was known. In the current article, we extend these results to the case in which no events in the training sample are labeled and also to the case in which the number of event types represented in the training sample is unknown. One can view the mixture approach as a robust method for fitting a density to training data that may not be normally distributed whether or not the data consist of identifiable components that have a physical interpretation. The technique is demonstrated using simulated data as well as two sets of seismic data.
Personalized rehabilitation of patients after COVID-19: the experience of the Republic of Mari El
Authors: Khamitov R. F. , Soboleva E. A.
Published: October 2022

Two years after the onset of the COVID-19 pandemic, not only the diagnosis and treatment, but also rehabilitation of patients at different times after the disease became relevant.

The aim. To develop and analyze the efficiency of personalized hospital-based rehabilitation programs for COVID-19 patients.

Methods. Retrospective analysis of medical records of 109 patients with COVID-19 admitted to the rehabilitation department. A team of physiotherapist, pulmonologist and psychiatrist used simulators (bicycle ergometer, treadmill, motomed, and stepper), physiotherapeutic exercises, training of respiratory muscles with Threshold IMT (Philips, Netherlands) simulators, psychotherapy, and physiotherapy.

Results. A total of 109 patients, 59 (54%) men and 50 (46%) women, aged 56.54 ± 11.73 уears suffered from moderate (41%), severe (40%) and very severe (19%) COVID-19. 25% of the patients had concomitant diabetes mellitus, 65% – arterial hypertension, and 22% – ischemic heart disease. Patients with 5 rehabilitation points (5RP) on Rehabilitation Routing Scale walked a median distance of 110 [40; 248] m in the 6-minute walk test (6MWT), 4RP – 300 [240; 350] m in 6MWT, 3RP — 400 [360; 431] m in 6MWT. The difference in 6MWT distance was significant only between 4RP and 3RP groups (p < 0.001). By the end of rehabilitation program, 6MWT increased by 90 m in 5RP group, by 120 m – in 4RP group, and by 89 m in patients with 3RP (p = 0.036 between RPs and 4RP groups; р = 0.007 between 3RP and 5RP groups, respectively). SpO2 was over 95% in all patients by the end of rehabilitation.

Conclusion. Comorbid patients after severe COVID-19 demonstrated better rehabilitation potential seen as improvement of exercise tolerance and respiratory status. The original personalized rehabilitation programs improve significantly impaired body functions early on after severe COVID-19.
Effects of physical rehabilitation on exercise tolerance in post-COVID patients: results of an open controlled trial
Authors: Chikina S. Yu. , Kuleshov A. V. , Nikitina N. V. , Meshcheryakova N. N.
Published: October 2022

The issue of physical rehabilitation of post-COVID patients stays relevant up to this day. Most publications discuss inpatient rehabilitation and only few publications describe outpatient physical rehabilitation of such patients.

The aim. Our study was performed to investigate efficacy of outpatient physical rehabilitation of post-COVID patients with lung injury.

Methods. This was a prospective non-randomized open controlled study. All patients with COVID-19-associated lung injury were referred to supervised outpatient rehabilitation program. We analyzed 6-minute walk test (6-MWT) results, evaluation of dyspnea using Borg scale and assessment of oxygen saturation (SpO2) using pulse oxymeter before and after walking.

Results. The rehabilitation group included 24 patients and the control group included 6 patients. The baseline and demographic findings did not differ significantly between the groups. The 6-minute distance (6-MWD) (р = 0.000018), heat rate at rest (р = 0.017) and after walking (р = 0.017), dyspnea after walking (р = 0.017), oxygen saturation at rest (р = 0.030) and after walking (р = 0.0021) improved significantly in the rehabilitation group and did not change significantly in the control group. At the end of the study, 6-MWD increased by 63.2 ± 36.3 m in the rehabilitation group compared to 14.0 ± 28.8 m in the control group (р = 0,01) and exertional dyspnea score increased by 0 (0 – 1) compared to 1 (1 – 2) in the control group (p = 0.033).

Conclusion. Supervised outpatient physical rehabilitation in outpatient settings can accelerate physical recovery in post-COVID patients with lung injury.
Chronic obstructive pulmonary disease and COVID-19
Authors: Zaryanova E. A. , Osipova G. L. , Osipova V. V.
Published: October 2022

Recently, single studies have described the picture of COVID-19 in patients with chronic obstructive pulmonary disease (COPD). Further study of this comorbid condition will help to assess the course and prognosis of each condition correctly, develop an effective plan for the management of patients with COPD during the pandemic caused by SARS-CoV-2 infection.

The aim. The article is an analytical review of the scientific literature from PubMed, Google Scholar, medRxiv, bioRxiv in order to study the clinical features of COVID-19 in patients with COPD. The published studies of the new coronavirus infection showed that patients with COPD account for 2% among those infected with SARS-CoV-2. However, further studies are needed to study the course of COVID-19 in patients with COPD. The conducted studies indicate a high level of ACE2 receptors to SARS-CoV-2 in current smokers and patients with COPD, which may explain the easier penetration of SARS-CoV-2 into the host organism and the severe course of the disease in this population.

Conclusion. Although a number of clinical studies strongly suggest that men have more severe COVID-19 than women, the patient’s gender is not always taken into account. It is possible that the more severe course of COVID-19 is associated with the increased prevalence of smoking and COPD in men as compared to women to a lesser extent than with the high production of testosterone and X-linked inheritance of the androgen gene and the ACE2 gene.
A case of severe COVID-19 in a patient with progressive fibrosing interstitial lung disease
Authors: Akulkina L. A. , Brovko M. Yu. , Schepalina A. A. , Sholomova V. I. , Kitbalian A. A. , Moiseev A. S. , Potapov P. P. , Moiseev S. V.
Published: October 2022

Interstitial lung diseases (ILDs) is a wide group of diffuse parenchymal lung diseases that can lead to interstitial pulmonary fibrosis. Clinical course of all ILDs, in particular with chronic fibrosing phenotype, can be complicated by an acute exacerbation caused by infection. Today, data about clinical course of COVID-19 in patients with progressive interstitial lung diseases is limited. In this case we described the course of COVID-19 infection in a patient with an interstitial pneumonia with autoimmune features (IPAF) and a progressive pulmonary fibrosis (PPF). We also discussed the typical features of COVID-19 in this population and the directions for further research.
Microbiome and Allergy: New Insights and Perspectives
Authors: Zubeldia-Varela E , Barker-Tejeda TC , Obeso D , Villaseñor A , Barber D , Pérez-Gordo M
Published: October 2022

The role of the microbiome in the molecular mechanisms underlying allergy has become highly relevant in recent years. Studies are increasingly suggesting that altered composition of the microbiota, or dysbiosis, may result in local and systemic alteration of the immune response to specific allergens. In this regard, a link has been established between lung microbiota and respiratory allergy, between skin microbiota and atopic dermatitis, and between gut microbiota and food allergy. The composition of the human microbiota is dynamic and depends on host-associated factors such as diet, diseases, and lifestyle. Omics are the techniques of choice for the analysis and understanding of the microbiota. Microbiota analysis techniques have advanced considerably in recent decades, and the need for multiple approaches to explore and comprehend multifactorial diseases, including allergy, has increased. Thus, more and more studies are proposing mechanisms for intervention in the microbiota. In this review, we present the latest advances with respect to the human microbiota in the literature, focusing on the intestinal, cutaneous, and respiratory microbiota. We discuss the relationship between the microbiome and the immune system, with emphasis on allergic diseases. Finally, we discuss the main technologies for the study of the microbiome and interventions targeting the microbiota for prevention of allergy.
Computational Component in a Hemispherical Resonator Gyroscope with its Measuring Component Utilising Alternating Voltage
Authors: Trutnev G.A. , Shchenyatskiy A.V.
Published: October 2022

The paper considers a hemispherical resonator gyroscope. It describes the structure of a hemispherical resonator gyroscope as a measuring and computing system, as well as the problems and main factors involved in increasing its accuracy. We studied a measuring component utilising alternating voltage, its circuit layout and mathematical model. We stated the problem of synthesising a computational component taking into account possible computational resource limitations in the device in the form of a system of nonlinear algebraic equations. We considered the specifics and implementation requirements concerning the computational algorithm, as well as the specifics of utilising redundant information about the wave pattern state in the gyroscope. The equation system proposed is overdetermined. We analysed the issues peculiar to the iterative method of solving this nonlinear system, which we propose to use as the basis for the mathematical model of the computational component. The method described may be generalised as the Gauss --- Newton method. The paper provides alternative iterative methods for designing the computational component. We investigated convergence issues for the computational component model obtained via the Gauss --- Newton method. The paper presents computational component simulation results and concludes that using the computational component model obtained is possible and feasible
Estatus ácido-base
Authors: Dalmau Barros Ernesto Andrés , Trujillo Jurado Carlos Andrés , Venegas Cortés Carlos Alberto
Published: October 2022

El tema central de esta obra es el mantenimiento de la condición ácido-base, los desequilibrios y la compensación de las alteraciones en caninos, bovinos y equinos. A partir del estudio de los sistemas renal y respiratorio, mediante la revisión de aspectos y definiciones básicos, se tratan las funciones más relevantes de cada sistema para entender las alteraciones ácido-base primarias y los cambios secundarios o compensaciones. El análisis tradicional se hace a partir de los resultados de química sanguínea, teniendo en cuenta la ecuación de Henderson Hasselbalch. Finalmente, el estatus ácido-base se aborda con las ecuaciones de Stewart, las cuales son tomadas como un método alternativo para establecer la condición ácido-base de un paciente, a partir de variables independientes y dependientes.
Shaping Effects on Long Span Bridge Deck Aerodynamics
Authors: ETEMADİ Ali
Published: October 2022

An aerodynamic circumstance of wind pressure surrounding the long-span bridge allocates many theoretical and experimental research to this topic. Determination of the materials and optimal cross-sectional shape of bridge decks that affected a dynamic behavior of long span bridge deck is still included in current research issues and works to be continued in this path. These include the Lack of sufficient awareness of wind forces, stemming from complex nature, and the unpredictability of the wind nature. In this study, in addition to recognizing the aerodynamic behavior of the flutter, the acting pressure forces on the bridge deck are investigated. The geometrical shape of decks, wind velocity, and flutter conditions are adopted as design variables that affected the dynamic forces exerted on bridge decks. A common type of geometric sections of the long-span bridge deck and effective aerodynamic phenomena are examined. The hollow box steel suspended deck and double cells box girder linked via upper flanges and cells linked via the top and bottom flanges are adopted for Computational Fluid Dynamic (CFD) approach. Thus, aerodynamic instability and turbulent torsional flutter flows, as well as a trail of shedding vortices around the bridge decks, are investigated. By changing some geometrical parameters of commonly used bridge sections, the optimal cross-section in terms of turbulence created above and below the deck section is examined and an optimal cross-sectional shape variable is proposed. The shape variable and section dimensions adopted for CFD-Simulations are similar to the dimensions and materials used in previous laboratory specimens of wind tunnels to be able to interpret the results and possibly verify them with the result of the current study.
Parameter Optimization of Bidirectional Three-Phase DC-AC Power Inverter by an Improved Particle Swarm Optimization based Fractional Order PI Controller for the Grid Forming Operation
Authors: ÇELEBİ Mehmed , BAŞÇİ Abdullah
Published: October 2022

Nowadays, it is becoming more remarkable for bidirectional power conversion from battery energy sources. These structures can be used for three-phase loads and they also have a common application in Micro-grid systems. This paper is mainly focused on parameter optimization of the system by using improved particle swarm optimization (PSO) in grid forming operation of dual active bridge series resonant converter (DABSRC) topology, in term of total harmonic distortion (THD). Fractional order proportional integral (FOPI) controller is used to simulate DABSRC regarding to THD factor in this system. The results are also compared to genetic algorithm (GA) and conventional PSO. While all algorithms achieved the same optimum control parameters, a significant reduction of computation time has been reported by improved PSO in terms of THD.
Improving the Ability of Teachers in Utilizing the Surrounding Environment as a Learning Resource in the Era of the Covid-19 Pandemic Through In-House Training at SD Negeri Banjarharjo, Kalibawang, Kulon Progo in 2021
Authors: Kandibah Umi
Published: October 2022

The research aims to improve the ability of teachers to utilize the surrounding environment as a learning resource and increase the professionalism of school principals in the era of the COVID-19 pandemic. The method used in this research is analytical descriptive, which is a study to collect data, describe, process, analyze, interpret and conclude data so that a systematic picture is obtained. Document data collection, observation, and interviews. The research subjects were 9 teachers of Banjarharjo Public Elementary School, Kalibawang Kulon Progo consisting of 6 class teachers and 3 subject teachers. The data collection in this school action research was carried out using instruments consisting of: 1) an instrument for observing participants' abilities, and 2 ) the ability of researchers in managing IHT. The data analysis used in this research is descriptive quantitative data analysis. The expected target in this study is that teachers are able to utilize learning resources with the surrounding environment. The results of the study on the ability of teachers to use the surrounding environment as a learning resource, it was found that an increase in the average score of the first cycle to the second cycle reached 0.50, while based on the percentage there was an increase of 12.45%, with the last average percentage of the second cycle reaching 87 .89%, thus the level of teacher ability has shown a significant increase above the indicator of success, 85%, when viewed from the results based on the conversion of the average value of the second cycle reaching 4, with the "very good" category. Furthermore, the results of research related to the management of the implementation of In House Training (IHT) by researchers in delivering material using the surrounding environment as a learning resource, the results obtained are the average score in cycle I; 3.29, with a percentage of 82.12%, the conversion value reached; 3, with the "Satisfactory" category, while in the second cycle the research results total average score of 3.59, based on a percentage of 89.65%, value conversion; 4, with the category "Very Satisfactory", when compared to cycle I; there is an increase; 7.53%, if it is concluded that the implementation of IHT can have a positive impact on increasing the ability of participants to utilize learning resources using the surrounding environment.
 
Keywords: Learning Resources, the surrounding environment, In House Training (IHT)
Virtual Reality Destination Experiences Model: A Moderating variable between Wisesa Sustainable Tourism Behavior and Tourists' Intention to Visit.
Authors: Wismantoro Yohan , Aryanto Vincent Didiek Wiet , Pamungkas Imang Dapit , Purusa Nanda Adhi , Amron Amron , Chasanah Amalia Nur
Published: October 2022

Abstract: This research aims at developing a virtual reality destination experience (VRDE) model as a moderating variable between sustainable tourist behavior (STB) and tourist visit intention (TVI). The portability variable strengthens VRDE, and the content quality variable supports VRDE. The research method used was purposive convenience sampling covering 204 respondents who visited the vicinity of Kebon batik village in Klaten, Central Java, Indonesia. The research findings were H1: STB has a significant effect on VRDE, H2: VDRE has an impact on TRVI, H3: portability has a significant effect on VRDE, and H4: content quality did not have an impact on VRDE. The novelty of this research is the VRDE application applied to Community Sustainable Tourist Behavior in developing countries.
Pengaruh Likuiditas, Profitabilitas, Dan Solvabilitas Terhadap Kebijakan Dividen Pada Perusahaan Otomotif Yang Terdaftar Di Bursa Efek Indonesia Periode 2016-2020
Authors: Rizkiyeh Rizkiyeh , Sudarsono Bambang
Published: October 2022

Effect of Liquidity, Profitability, and Solvency on Dividend Policy in Automotive Companies Listed on the Indonesia Stock Exchange (IDX) 2016-2020.  Under the guidance of  Drs.  Bambang Sudarsono, MM.This study aims to determine the effect of liquidity, profitability, and solvency variables on dividend policy in automotive companies listed on the Indonesia Stock Exchange (IDX) in 2015-2020.The approach used in this research is quantitative research.  The number of samples in this study were 7 companies.  The data analysis technique used in this research is multiple linear regression analysis, with hypothesis testing statistical t-test and statistical f-test.Based on the research that has been done, the results show that liquidity and profitability have a significant positive effect on dividend policy, while solvency has no effect on dividend policy, but liquidity, profitability and solvency have a simultaneous positive and significant effect on dividend policy.
Wisata Halal Madura: Strategi Destinasi untuk Daya Saing Pasar Pariwisata pasca Pandemi Covid 19
Authors: Faidal Faidal
Published: October 2022

The purpose of this research is to conceptualize the competitiveness of Madura halal tourism village destinations, formulate strategies to manage halal tourism village destinations effectively to help management make policies and for practitioners to better understand the advantages and problems of halal tourism village destinations and the development of halal tourism in Madura. especially after the COVID-19 pandemic.Methods This research uses a mix method with triangulation (case study design and survey design). For qualitative, data were collected from a number of tourist village destinations through observation, in-depth interviews, and FGD methods.The competitiveness of Madura as a halal tourism village destination is based on 7 sub-variables including: tourist attractions, most of the respondents tend to state that they are in good condition. as well as from the aspect of accessibility, amenities and institutions, that overall, respondents tend to say they are ready. The accommodations available in Madura as a whole have implemented halal principles in their services. need commitment and consistency in working on halal tourism in Madura in order to be competitive with other tours.
TARIAN RANUP LAMPUAN: MENINGKATKAN PERKEMBANGAN FISIK MOTORIK KASAR ANAK USIA DINI
Authors: Tursina Ade , Mahriza Rita , Ramaida Agus
Published: October 2022

Perkembangan fisik motorik kasar menjadi penting bagi anak usia dini karena berkaitan dengan aspek perkembangan lainnya seperti kepercayaan diri maupun konsep diri, sehingga perlu stimulasi yang tepat. Penelitian ini bertujuan untuk mengetahui bagaimana kegiatan guru dalam upaya meningkatkan kemampuan motorik kasar melalui Tarian Ranup Lampuan di TK Islam Nasurullah dan untuk mengetahui bagaimana peningkatan perkembangan motorik kasar anak melalui Tarian Ranup Lampuan di TK Islam Nasurullah. Metode penelitian yang digunakan adalah penelitian tindakan kelas (action classroom research) melalui dua siklus dengan melibatkan sebanyak 17 anak pada kelompok B di TK Islam Nasurullah.  Hasil penelitian ini berupa peningkatan kemampuan guru dalam mengoptimalkan kemampuan motorik kasar anak melalui tarian Ranup Lampuan. Hal ini tampak dari keseluruhan kemampuan guru yaitu siklus I mendapat nilai rata-rata 3,3 dengan kategori “Baik” dan pada siklus II yaitu dengan nilai rata-rata 4,0 dengan kategori “Sangat Baik” dan Tarian Ranup Lampuan dapat meningkatkan kemampuan motorik kasar anak kelompok B1. Apabila dilihat dari hasil rata-rata ketercapaian anak pada siklus 1 yaitu 57,2%, dengan kriteria “Berkembang Sesuai Harapan”. Sedangkan pada siklus II anak meningkat mencapai nilai rata-rata 86,6% dengan kriteria “Berkembang Sangat Baik”. Hal ini telah mencapai kriteria keberhasilan yakni 80%.
En-claves relacionales presentes en Recuerdo, repetición y elaboración (Freud, 1914) – Segunda parte: Las palabras de Freud
Authors: Tinajas Puertas Antonio A.
Published: October 2022

Subjected to the historical-critical method, and resorting to the content analysis technique, this work takes as a reference the Freudian technical study known as Remembrance, Repetition and Elaboration (Freud, 1914) to substantiate that certain concepts, precepts, or therapeutic lines of action – that is, theoretical-technical ideas- ascribed to the so-called psycho-relational analysis, are already present in the programmatic Freudian study written in 1914.
Isolation of Total RNA with the Zymo Quick-RNA Miniprep Kit
Authors: Vitucci Eva CM , McCullough Shaun D.
Published: October 2022

This protocol describes the use of the ZYMO Quick-RNA miniprep kit for the isolation and purification of total RNA from cells grown in culture. The conditions described below were developed for the isolation of RNA from H441 and HULEC from the alveolar-capillary region exposure (ACRE) model. Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use. NOTE: There is a consolidated PDF version of this protocol in the supplementary files section below.
Ocean variability beneath Thwaites Eastern Ice Shelf driven by the Pine Island Bay Gyre strength
Authors: Dotto Tiago Segabinazzi , Heywood Karen , Hall Rob , Scambos Ted , Zheng Yixi , Nakayama Yoshihiro , Hyogo Shuntaro , Snow Tasha , Wahlin A. K. , Wild Christian , Truffer Martin , Muto Atsuhiro , Alley Karen , Boehme Lars , Bortolotto Guilherme , Tyler Scott , Pettit Erin
Published: October 2022

West Antarctic ice shelf thinning is primarily caused by ocean-driven basal melting. Here we assess ocean variability below Thwaites Eastern Ice Shelf (TEIS) and reveal the importance of local ocean circulation and sea-ice. Measurements obtained from two sub-ice-shelf moorings, spanning January 2020 to March 2021, show warming of the ice shelf cavity and an increase in meltwater fraction of the upper sub-ice layer. Combined with ocean modelling results, our observations suggest that meltwater from Pine Island Ice Shelf feeds into the TEIS cavity, adding to horizontal heat transport there. We propose that a weakening of the Pine Island Bay gyre caused by prolonged sea-ice cover from April 2020 to March 2021 allowed meltwater-enriched waters to enter the TEIS cavity, which increased the temperature of the upper layer. Our study highlights the sensitivity of ocean circulation beneath ice shelves to local atmosphere-sea-ice-ocean forcing in neighbouring open oceans.
Dynamic behavior of a class of predator-prey model with two time delays
Authors: Peng Yuhui , Ren Meirong , Qian Youhua
Published: October 2022

In this paper, three modified Lesile-Gower predator-prey models with two time delays are considered. Taking the time delay as bifurcation parameter, when Hopf bifurcation occurs, the critical value corresponding to time delay is obtained. By using normal form theory and central manifold argument, the direction of Hopf bifurcation and the stability of bifurcation periodic solution can be determined. Finally, numerical simulation is performed to support theoretical analysis.
CUR5g, a novel autophagy inhibitor, exhibits potent synergistic anti-cancer effects with cisplatin against non-small-cell lung cancer
Authors: Zhang Lu , Chen Jingxuan , Shen Yunpeng , Wu Bowen , Yang Peichang , Sun Gangchun , Liu Xiaoting , Qiang Pengfei , Gao Yamei , Sha Fangfang , Li Zirui
Published: October 2022

Autophagy, a highly conserved degradation process of eukaryotic cells, has been proved to be closely related to chemoresistance and metastasis of non-small-cell lung cancer (NSCLC). Autophagy inhibitors, such as chloroquine (CQ) and its derivative hydroxychloroquine (HCQ), has been shown to mediate anti-cancer effects in preclinical models, especially when combined with chemotherapy. However, the vast majority of autophagy inhibitors, including CQ and HCQ, actually disrupt lysosomal or/and possibly non-lysosomal processes other than autophagy. It is therefore of great significance to discover more specific autophagy inhibitors. In this study, after screening a series of curcumin derivatives synthesized in our laboratory, we found that (3E,5E)-1-methyl-3-(4-hydroxybenzylidene)-5-(3-indolymethylene)-piperidine-4-one (CUR5g) selectively inhibited autophagosome degradation in cancer cells by blocking autophagosome-lysosome fusion. CUR5g did not affect the lysosomal pH and proteolytic function, nor did it disturb cytoskeleton. CUR5g blocked the recruitment of STX17, a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein, to autophagosomesviaa UVRAG-dependent mechanism, resulting in the inability of autophagosomes to fuse with lysosomes. CUR5g alone did not induce apoptosis and necrosis of A549 cells, but significantly inhibited the mobility and colony formation of A549 cells. More excitingly, CUR5g showed no obvious toxicity to normal HUVECs invitroor mice invivo. CUR5g enhances the cisplatin sensitivity of A549 cells and effectively inhibited autophagy in tumor tissues invivo. Collectively, our study identified a new late-stage autophagy inhibitor and provided a novel option for NSCLC treatment, particular when combined with cisplatin.
Selected River Pollution in Bangladesh Based on Industrial Growth and Economic Perspective: A Review
Authors: Miah Md Helal , Chand Dharmahinder Singh , Malhi Gurmail Singh
Published: October 2022

The main goal of sustainable development is to engage the public in setting the groundwork for developing profiles based on carrying capacity assessments. Since industrial projects are located in traditional, non-industrial zones, the broad human resource development program includes environmental research, education, and training to build technical and practical skills in the country-based and scientific statistics system data gathering. It is noteworthy that the examinations were conducted sporadically and that the research did not correspond to the pollution level in Bangladesh's waterways. Therefore, it is essential to conduct a methodical examination that may offer a complete picture of river pollution so that appropriate preventative actions can be adopted to safeguard against pollution threats. Bangladesh has many environmental issues, including dirty air from various vehicles, unhealthy water, accumulating urban waste, untreated sewage, interior air pollution from wood smoke, and usage of fad-driven contemporary materials in homes. Each of them contributes more to the destruction of the environment. With the current trend of population growth followed by the construction of companies to suit their wants, such a situation may worsen. Hazardous gases, dust particles, or liquid effluents are the waste products emitted from industrial sources. These discharges are full of harmful chemicals that pollute aquatic ecosystems, disrupt the local biota, and eventually harm living things and associated flora and animals. As a result, studies were conducted, including the physicochemical characterisation of sugar refineries, distilleries, and other rivers that receive such effluents. These characteristics of subsurface water were also taken into consideration throughout the inquiry. This research will be anticipated to offer proper preventive methods for preserving the purity of the Bangladeshi rivers.
Efficacy of Bacopa monnieri on memory and vascular functions: A randomised controlled trial
Authors: Kamkaew Natakorn , Ingkaninan Kornkanok , Waranuch Neti , Kaewmahanin Watchara , Wasuntarawat Chanchira , Khongsombat Onrawee , Thiarawat Peeraphong , Roongpiboonsopit Duangnapa , Wattanathorn Jintanaporn , Scholfield C. Norman , Ngamdokmai Ngamrayu , Wisutthathum Sutthinee , Chatturong Usana , Inchan Anjaree , Chootip Krongkarn
Published: October 2022

Bacopa monnieri (L.) Wettst. (Brahmi) is a traditional memory enhancer partly by improved cerebral blood flow. Here we sought to link improved cognitive function with better blood flow in randomised double-blinded placebo-controlled trial in an elderly cohort. Normotensive Thais, aged 55-80y having mini-mental state examination (MMSE) scores > 25, no dementia or other psycho/neurological disease, normal lipid profile, and blood biochemistry were recruited. The trial design was a 2 week run-in, 12 week intervention of test product or placebo, and 4 week washout. The intervention was an extract ofB. monnierileaves (eBM) in 40 ml of mulberry juice. The placebo contained mulberry juice and other constituents to match gustatory properties. End-points were a battery of memory functions, carotid blood velocity, post-ischemic microvascular blood flow, markers of vascular inflammation, blood pressure and the blood markers. Response latency was reduced by 14.2 ± 4.9% (p = 0.022 comparing placebo) but only in > 65s. Other memory recall parameters were either unaffected or for ‘accuracy of recall’ was already maximal preventing further improvement. No change was detected in carotid blood velocity while microvascular blood flow marginally increased (by 28.4 ± 8.3%,p = 0.07). This preliminary evidence warrant further studies on selected patients with microvascular cognitive dysfunction using more discriminating protocols.
Clinical predictors and management for radial artery spasm: An Australian cross sectional study
Authors: Curtis Elizabeth , Fernandez Ritin , Khoo John , Weaver James , Lee Astin , Halcomb Elizabeth
Published: October 2022

Introduction
The transradial approach for coronary artery catheterisation has increased in popularity compared to the transfemoral approach for patients undergoing percutaneous coronary interventions. However, radial artery spasm continues to be a major complication of the procedure.
Aim
To identify the predictors of radial artery spasm and the medications used for its prevention and management.
Methods
A descriptive cross-sectional study was carried out across two tertiary hospitals in NSW, Australia. A self-administered pre-procedural survey was completed by patients undergoing coronary artery catheterisation. This survey collected socio-demographic data and assessed anxiety using the Spielberger State-Trait Anxiety Inventory. Procedural data, including length of procedure, equipment used, occurrence of radial artery spasm, and medications given, were collected post-procedure by the interventionalist.
Results
Of the 169 participants, over half were male (59.8%) and aged 66 years or older (56.8%). Radial artery spasm was reported in 24 (14.2%) participants. Rates of spasm were significantly higher among females (66.6%, p=0.004), those aged under 65 years (62.5%, p=0.001) and those who reported a medical history of anxiety (33.3%, p=0.0004). There were no significant differences in State and Trait anxiety scores among those who had RAS and those who did not. Logistic regression identified younger age as a statistically significant predictor of RAS (OR 0.536; 95% CI 0.171 to 1.684; p=0.005)
To prevent radial artery spasm most patients received midazolam (n=158; 93.5%), nitrates (n=133; 78.7%) and/or fentanyl (n=124; 73.4%) prophylactically. Nitrates were the most frequently administered medication to treat radial artery spasm (78.7%).
Conclusion
This study found greater clarity around predictors for RAS is required, as identifying patients at risk can ensure prophylactic measures are implemented. This study identified nitrates as the preferred vasodilator as a preventative measure along with the use of sedation.
Robust Advanced Modeling and Scheduling System (RAMS): From Space Exploration to Real-World Biomanufacturing
Authors: Saint-Guillain Michael , Sansdrap Isabelle , Brion Michaël , Pauly Eric
Published: October 2022

Advanced planning and scheduling (APS) systems aim at helping production and operation managers in organizing the manufacturing process of biotech products, finding near-optimal schedules meeting the demand, while taking operational resources and raw materials into account. We introduce an extension to APS, called Robust Advanced Modeling and Scheduling (RAMS), and present the first RAMS system: Rombio. Unlike existing APS systems, Rombio allows to (i) visually model the operational problem and context entirely (ii) generate and optimize schedules while taking uncertainty into account, and (iii) deal with a combination of various key performance indicators (KPIs). Probability theory enables us to cope with uncertainty, computing schedules that are robust to temporal deviations. Depending on the pursued KPIs, the resulting schedules optimize a combination of the following terms: the probability of satisfying the process constraints, the expected return/efficiency/quality, and even the operators’ wellness by minimizing its expected extra-hours. This introduces a new risk-aversion paradigm, replacing the well-known what-if and sensitivity analysis frameworks. Initially developed in collaboration with Nasa for space exploration, this versatile tool is applied to real-world biotechnology manufacturing in three different contexts: diagnostics, medicines and stem cells.
The Association of Regional Block with Intraoperative Opioid Consumption in Patients Undergoing Video-Assisted Thoracoscopic Surgery: a single-center, retrospective study
Authors: Yan Xiang , Liang Chen , Jiang Jia , Fu Yili , Wei Changwei
Published: October 2022

BACKGROUND: Regional block, such as thoracic epidural analgesia (TEA), thoracic paravertebral block (TPVB), or serratus anterior plane block (SAPB) has been recommended to reduce postoperative opioid use in recent guidelines, but the optimal options for intraoperative opioid minimization remain unclear. The aim of this study was to evaluate the intraoperative opioids-sparing effects of three regional blocks (TEA, TPVB, and SAPB) in patients undergoing video-assisted thoracoscopic surgery (VATs).
METHODS: This was a retrospective study of the adults undergoing VATs at a tertiary medical center between January 2020 and February 2022. According to the type of regional block used, patients were classified into 4 groups: GA group (general anesthesia without any regional block), TEA group (general anesthesia combined with TEA), TPVB group (general anesthesia combined with TPVB), and SAPB group (general anesthesia combined with SAPB). Cases were matched with a 1:1:1:1 ratio for analysis by age, sex, ASA physical status, and operation duration. The primary outcome was the total intraoperative opioid consumption standardized to Oral Morphine Equivalents (OME). Multivariable linear regression was used to estimate the association of the three regional blocks with the OME.
RESULTS: A total of 2159 cases met the eligibility criteria. After matching, 168 cases (42 in each group) were included in analysis. Compared with GA without any reginal block, the use of TEA, TPVB, and SAPB reduced the median of intraoperative OME by 78.45 mg (95% confidence interval [CI], -141.34 to -15.56; P=0.014), 94.92 mg (95% CI, -154.48 to -35.36; P=0.020), and 11.47mg (95% CI, -72.07 to 49.14; P=0.711), respectively.
CONCLUSIONS: The use of TEA or TPVB was associated with an intraoperative opioid-sparing effect in adults undergoing VATs, whereas the intraoperative opioid-sparing effect of SAPB was not yet clear.