bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploratory Analysis Affecting Self-Efficacy in the Leadership of Female Village Heads
Authors: Wati Sri Susilawati , Sukmono Rita Ambarwati
Published: September 2021

Leadership is one of the most decisive factors in the development and progress of an organization. With a capable leadership that will have an impact on the progress of the organization. Public leadership by women has less legitimacy by society. Self-leadership has a significant effect on self-efficacy, self-efficacy has a significant effect on performance, self-leadership has a significant effect on performance. In order to optimize the ability of women's public leadership, self-efficacy in leadership by female headman indirectly affects the quality of leadership and service to the community. This study aims to explore the self-efficacy of female headman in the region and identify factors that influence the self-efficacy of female headman when in office as village government. This research was conducted through a self-administered questionnaire. In addition, exploration and confirmation of factor analysis were carried out. The validity and reliability tests were carried out before using exploratory factor analysis (EFA). The results of the factoring will show the communalities table, and after the rotation, a loading factor will be formed which will show the interpretation of the factors that have been formed, in particular giving names to the factors that are considered to represent these indicators. The results showed that 2 factors were formed which influenced the self-efficacy of female headman in leading village officials. These factors are Leadership Self Efficacy and Development of the Leadership Self-efficacy. The factor 1 is namely Leadership Self Efficacy which includes leading change, delegating tasks, influencing members, being flexible. Factor 2, namely Development of the Leadership Self-efficacy which includes self-identification, motivating members, leading the organization, and encouraging the organization.
Application Development 2013 Curriculum Report
Authors: Asy’Ari Mochammad Hasyim , Untari Rahmania Sri , Hasanah Fitria Nur
Published: September 2021

In SMA Dian Indonesia the report card data entry process is still constrained by the complexity of the application flow that is used. Each subject teacher gives a grade to the homeroom teacher to be processed into a report card. This made it difficult for the homeroom teacher to process the data provided by the subject teacher so that the process of processing report cards was hampered."This study aims to determine the process of developing the 2013 Curriculum Report Card Processing Application at Dian Indonesia High School. Based on the results of the analysis, it was obtained 90% in the very feasible category by media experts, and from the teacher the average validity was obtained 80.7% with the feasible category. So it can be concluded that the development of the 2013 Curriculum Report Card Processing Application is very suitable for use at Dian Indonesia High School.
Desingning An E-Voting Information System For Website-Based Village Head Elections
Authors: Kurniawan Aditya , Findawati Yulian
Published: September 2021

Voting in a democratic country is an important part of the means of choosing leaders. The village head election process in Indonesia still uses conventional voting methods, namely using ballot paper media in the election process. Voting that is carried out conventionally has several obstacles, including the lack of guaranteeing the authenticity of voters' votes, so that people think the results of voting results are often manipulated. In addition conventional selection is deemed inaccurate and time-consuming and costly. In this study the aim of this research is to design an information system e-voting that can be used for the Election of the Village Head of Cemandi, Sedati, Sidoarjo, East Java, where by using this system the election process becomes easier by ensuring the accuracy of the vote count. This system development method uses the model of software engineering waterfall. The test results blackbox show that the functions of the features in the system are running well. Based on the UAT test, the system received an average percentage rate of 86%.
The Effect Of Transformational Leadership Style, Organizational Culture, And Competence On Job Satisfaction With Compensation As An Intervening Variable
Authors: Andrianto Risky , Harjatno Slamet
Published: September 2021

This study aims to examine the effect of transformational leadership style, organizational culture, and competence on job satisfaction with compensation as an intervening variable. This research is a descriptive study using quantitative methods. The sample used in this study amounted to 95 respondents using a saturated sampling method. The data collection technique used a questionnaire with a Likert scale. This study uses SEM analysis.

Based on the results of research with a hypothesis testing variables transformational leadership style, organizational culture, and competence on job satisfaction with compensation as an intervening variable. From the PLS test results, it is known that transformational leadership style has a direct effect on job satisfaction, Organizational Culture has a direct effect on job satisfaction, Competence has a direct effect on job satisfaction, Transformational leadership style has a direct effect on Compensation, Organizational Culture has a direct effect on Compensation, Competence has a direct effect on Compensation and Transformational leadership style, organizational culture and competence have an effect on job satisfaction through compensation.
Transactional Leadership Patterns For Human Resources Development
Authors: Bukron Ahmad , Haryanto Budi
Published: September 2021

The pattern of  leadership is a style or model of leadership used by leaders to influence subordinates so that organizational goals are achieved or it can also be said that leadership style is a pattern of behavior and strategies that are preferred and often applied by a leader. A leader in an educational institution must have a style or model in order to influence his subordinates in order to achieve goals at the institution. The transactional leadership pattern can be interpreted as the method used by a leader in moving his members by offering rewards/consequences for each contribution made by members to the organization.

                The focus of this research is to find out the leadership pattern of a madrasah principal in his efforts to develop human resources, in this case educators and education staff. Because in reality, madrasas are still seen as educational institutions that are less good than public schools. The progress of an educational institution cannot be separated from the participation of the principal, educators, teaching staff and parents of students. The author sees the leadership pattern of the head of MI Muhammadiyah 2 Kedungbanteng Tanggulangin with a transactional leadership pattern

This study uses a descriptive method which is carried out through data collection in the field, which provides an overview of the leadership of the madrasah principal. This research approach uses a qualitative approach. The data of this research are in the form of quotes, words, phrases, and story sentences. 

From the research conducted, MI Muhammadiyah 2 Kedungbanteng has not yet fully implemented the pattern of transactional leadership, because the exiting human resources tend to stagnate and do not develop in terms of academic graduates who are generally reluctant tot continue their studie at a higher level.
Social Loafing On Students Of Muhammadiyah University Sidoarjo
Authors: Oktrivia Ranti Jihan , Maryam Effy Wardati
Published: September 2021

This problem is motivated by the phenomenon of social loafing among students of the Muhammadiyah University of Sidoarjo. Social loafing is a lack of individual effort when working simultaneosly compared to when perfoming individual tasks. The purpose of this study was to determine the description of social loafing and the factors that cause students of the University of Muhammadiyah Sidoarjo to do descriptive quantitative social loafing. The population in this study were 9469 students of the University of Muhammadiyah Sidoarjo who were involved in the social loafing response at the University of Muhammadiyah Sidoarjo. The sample used was 355 students based on an error rate of 5% in the table developed by Isacc and Michael. The data collection technique in this study uses a psychological scale, which is a social loafing scale in the form of a used scale. Based on the results of the validity test 15 valid items from the 44 compiled items. The reliability test showed a value of (r = 0.919). Based on the results of the analysis showed that the high category of social loafing (15.5%), medium (68.4%) and low (16.1). Most of the factors that influence social learning are no evaluation or assessment of group performance. The social loafing factor is obtained when there is no individual contribution between groups and the unclear division of responsibilities.
Analysis of Portfolio Expected Returns as a Material Consideration for Investors in Choosing Investments in the Pandemic Period (Formation of Optimal Portfolios Using the Black Litterman Model on Hang Seng Stocks for the 2017-2019 Period)
Authors: Rahayu Wiji , Hariyanto Wiwit
Published: September 2021

. This study attempts to find out how a method of Black Litterman in the formation of stock portfolios. This research was conducted on the basis of increasing the number of investors' funds in the capital market for certain stocks, showing that it increases positive sentiment on stock investments compared to other investments. The Black Litterman Model method is one of the options that can be used in the formation of portfolio. The Black Litterman model method is a method that formulates the existence of an element of return equilibrium and investor views in an investment. By using the Black Litterman Model, investors can take advantage of all available information as the basis for forming a maximum portfolio. The object of this research is Hang Seng (HSI) stock price data for the period 2017 – 2019. The research sample is 35 companies. The results of this study resulted in 10 stocks included in the Black Litterman model portfolio with the expected return on the portfolio (which consisted of 10 stocks with the Black Litterman model) of 0.062387. Where the highest proportion of returns given by Shenzhou International Group Holdings Limited (SEHK: 2313) is 23% and the expected return is 0.017933. While the lowest level is occupied by New World Development Company Limited (SEHK: 17) with a proportion of 1% and an expected return of 0.000687.
Accounting Treatment In Increasing Accountability And Transparency Of Budget Management With The Concept Of Value For Money
Authors: Umami Devi Riza , Widodo Heri
Published: September 2021

This research aims to determine the accounting treatment in increasing accountability and transparency of budget management with the concept of Value for Money in Gelang Village.

This type of research is a qualitative research that uses data collection methods through interview, documentation, and observation. Types and sources of data using primary data and secobdary data. The technique of determining the informants in this study uses a purposive sampling technique, which means that the technique is intented to select informants according to the object under study.

The results of the study indicate that the management of APBDes in Gelang Village has carried out a system of planning, implementation, administration, reporting, and accountability according to the rules. The application of the concept of Value for Money in budget management has been implemented through the 3E (Economy, Efficiency, and Effectiveness) well in Gelang Village. It can also be said to be transparent, because the village government has realized the APBDes funds by placing the APBDes realization report in front of the village office and holding a village meeting to discuss the APBDes program development plan
Alokasi Waktu Wanita Pada Industri Kerajinan Anyaman Pandan Di Kota Sungai Penuh
Authors: Suandi Suandi , Damayanti Yusma , Jasminarni Jasminarni , Novita Trias
Published: September 2021

This research aims at examining how women allocate their time of working  in pandanus handicraft industry on Sungai Penuh City and to analyze affect women's time allocation in pandanus craft industry. The study was conducted in Kota Dian Village Hamparan Rawang District Sungai Penuh City. This study employed cross-section primary data. The data collection was conducted from June until October 2017 by interviewing 32 womens who were selected by cluster and purposive sampling. Data were tested by descriptive and multiple regression test. The results showed that the average time allocation of women in pandanus industry for 30 hours / week or 5 hours /day by working part-time. Women has dual-role in the household. From the regression analysis, two significant factors observed. These are the number of household members and the earnings of the household, while the age factor and level of education of women have no significant effect.
Pengembangan Kapasitas UKM Produk Sepatu dan Tas Kulit di Malang untuk Tembus Pasar Luar Negeri
Authors: Istutik Istutik
Published: September 2021

This program aims is to increase the capacity of craftsmen who are economically productive micro-groups. This community problems are lack of ability to manage production schedules, lack of ability to determine product prices, lack of ability to market products that rely solely on reseller buyers, and limited production capacity. The method of implementing activities to solve community problems is to provide various training, then proceed with mentoring. Sewing machine equipment assistance is provided to increase production capacity. The specific target of this program is to improve production management capabilities, determination of selling prices, marketing and soft skills of craftsmen. The impact of program implementation is increased production capacity, increased sales and income turnover, increased partner communication skills, diversity of marketing media, and community success in penetrating foreign markets.
Desain Ulang Program Bantuan Dana Bergulir Di Kabupaten Sampang
Authors: Prihantono Gigih
Published: September 2021

The purpose of microcredit is to encourage the growth of small and medium enterprise business and increasing prosperity by expanding public access to credit. Sampang Regency is one of the poorest regions in the East Java, where are very few microfinance institutions which provide the loan. However, 68% of the inhabitants have a loan that originated from informal sources (moneylenders, friends, or family), while only 5% who reported having a loan in the Bank. We are trying to help the local Government of Sampang to increased access of credit by redesigning Revolving Fund program (Dagulir) owned by the Government to increase the income of small and medium enterprise business.  The results show that credit Dagulir can work widely through risk management and investment at the level of the household and not through their efforts as originally targeted.
Memperbaiki Kinerja Pemasaran Melalui Pelatihan Kewirausahaan, Riset Pasar, Dan Inovasi Produk
Authors: Sulaeman Maman , Kusnandar Hasan Fahmi , Sundari Ristina Siti
Published: September 2021

The purpose of this research is to know and analyze the improvement of marketing performance through entrepreneurship training, market research, and product innovation at UMKM in East Priangan West Java Province. The research method used is survey method. Data collection was obtained through questionnaires and literature study. The population in this study is MSMEs in East Priangan West Java. Determination of sample using random counted 75 samples and data analysis used was path analysis. The results found that partially entrepreneurial training, market research and product innovation were able to improve marketing performance. Simultaneously entrepreneurship training, market research and product innovation can improve the performance of UMKM marketing in East Priangan of West Java province.
Pemanfaatan Biji Durian menjadi Oleh-Oleh Khas Kampar
Authors: Verawati Besti
Published: September 2021

Durian seeds can used as as valuable flour and not inferior to other flour. This is will increase the economic value and its utilization. Durian seeds contain enough starch, had 8.79% protein, 85% carbohydrates, 980 mg calcium and 1300 mg phosphorus. Durian seed flour can be added in the ingredients of foods such as doughnuts, cakes, semprong, rempeyek, and brownies. That can increase the economic value of durian seed. Family welfare construction group (PKK) of Batu Belah and the Tanjung Bungo village is viable organization empowering women to participate in local development. The activities will be conducted on the increasing knownledge and technology. Furthermore, in this Community there are several development efforts, among other things: 1). Theory to enhance the knowledge about durian seed. 2). Training in the processing of durian seed flour. 3) Increased the motivation of the community. Activities carried out to increase the motivation of the community is the cooking competition in a variety of durian seed flour  as kampar special souvenirs, 4) making of a Kampar special food reciepe book as souvenir. 5) marketing strategy. The results of the implementation of the activity that is a. Increased capacity in production system of making Durian Seed Flour; b. an increase in the production of Durian Seed Flour preparations; c. development of marketing strategy.
Corn Brownies Making Entrepreneurship Training in The Turi Distric
Authors: Wasito Wasito , Novida Dyah Hadi , Fitriani Ida
Published: September 2021

Community service programs in the form of Entrepreneurship training were carried out in Turi Village, Turirejo Village, Kedamean District, Gesik Regency on August 21, 2017. This activity is the flagship work program of the 2017 KKN management study program. The name of this activity is "Entrepreneurship Training for Making Corn Brownies" with participants PKK mothers and residents of Turi Hamlet. In the training, material with the concept of motivation and entrepreneurship was presented, as well as a cooking demonstration for making corn brownies. The aim of carrying out a work program on entrepreneurship socialization is to provide knowledge to the PKK group of women and Turi hamlet residents about the management and use of local materials such as corn to become entrepreneurial opportunities. Utilization of local potentials owned by the people of Turi hamlet can increase their income with creative capital in managing the agricultural products they have and especially increasing the selling value of corn.
Optimizing the Production and Sales Capacity of Fish Crackers in Sidoarjo, Indonesia
Authors: Rosnawati Emy , Multazam Mochammad Tanzil , Cahyana Atikha Sidhi
Published: September 2021

This paper aims to provide additional knowledge related to increased production and marketing for fish cracker craftsmen. The method used is the method of mentoring and workshops, with partners being fish cracker craftsmen in the sawohan area of ​​Sidoarjo. The result of this assistance is an increase in production and marketing from partners.
Corporate Governance dan Shariah Compliance Terhadap Financial Statement Fraud Pada Bank Umum Syariah
Authors: Desiana Lidia , Alfaridzie M. Rifky Ramadhon , Akbar Dinnul Alfian
Published: September 2021

This study tested the Influence of Corporate Governance and Shariah Compliance on Financial Statement Fraud in Sharia Commercial Banks in the Period 2015-2019. Independent variables in this study are Corporate Governance and shariah compliance projected with Islamic Income Ratio, Profit Sharing Ratio and Islamic Investment Ratio. While the dependent variable used is Financial Statement Fraud in sharia commercial banks. The population in this study was all Sharia Commercial Banks (BUS). Sampling techniques using purposive sampling method. The number of samples as many as 12 Islamic commercial banks with a lot of data 60. The results of this study show islamic income ratio affects Financial Statement Fraud, while Profit Sharing Ratio, Islamic Investment Ratio and Corporate Governance have no effect on Financial Statement Fraud.
The Design of the SBMPTN Tryout System on the Official Website of the Tutoring Institute “Road to PTN”
Authors: Ravisafitra Lalu Ryan , Indahsari Rina Dewi
Published: September 2021

The design of the Road to PTN Tutoring Institution official website serves to assist students in preparing for the SBMPTN, as well as complete information from Road to PTN’s social media; LINE, Instagram, Youtube and Twitter. On the website that is built there are several main features, those are simulation, a user can buy a tryout class by filling up the balance (top up) using a bank ATM, the user can also download the test results, see the rank results, and get the results of the talent interest analysis. Several predictions of campus rank and department are displayed in the premium tryout class including the leaderboard rank profile (top 10). Beside that there is information on the article menu which contains tips and motivation. Issues published are made via the admin dashboard including confirmation of user activity. In the tests carried out on the Road To PTN official website, users can use all the available features including, login, create an account, set up accounts, fill in balances, withdraw balances, perform simulations, view rank results, download test results and create articles or read articles. And the admin has been able to process the data on the website. This is evidenced by the results of user testing functional testing and admin functional testing which results in all features being successfully used as expected.
Design And Build Mesh Topology At Muhammadiyah University
Authors: M. Arsyil Adhi’im  , Arif Senja Fitrani
Published: September 2021

With the capacity of the Muhammadiyah University of Sidoarjo campus in 3 places and the use of the internet at the largest University in Sidoarjo and continues to grow every year, it becomes an obstacle if there is an internet blackout on one of the networks in one of the campuses with data on the use of Network Topology. So that buildings at universities that are quite vulnerable can be connected and exchange information locally quickly without worrying about an off condition at one of the internet network sources, wireless hardware has been developed which is a core component of a wireless mesh network that promises high flexibility, reliability, and performance. The concept used in communication is the same between nodes, but there are some differences that are owned by having an academic background and focusing on device users, mobility, and capabilities in the form of implementing wireless technology which is one solution for providing telecommunications infrastructure that can be deployed easily. fast. In this study, the use of the Wireless Mesh Point-To-Point topology system in order to overcome network conditions on one of the networks at the University in particular experienced an off/outage condition.
Learning Media Applications and Introduction of Javanese Gamelan Musical Instruments Based on Android
Authors: Ariefbillah Muchammad Sofyan , Taurusta Cindy
Published: September 2021

The role of technology, especially information technology, is very helpful in people's lives, from the aspects of education, business, to the health sector. In this day and age, many modern musical instruments have begun to shift the existence of traditional music, especially this typical Javanese musical instrument, namely Gamelan. With the development of the times, the public's interest in this traditional Gamelan instrument is decreasing. So that this application has a problem formulation, namely how to design and create an android application that concerns the introduction and learning of various types of gamelan musical instruments. This study aims to produce an application for the introduction of an Android-based gamelan instrument that can be used by all groups to be more familiar with and interested in learning and preserving gamelan instruments. This research was conducted with a survey method to obtain data or opinions to test this application. Based on the research that has been done, a conclusion can be drawn with this research which results in an application that can help and make it easier for people to know the various types of Javanese gamelan.
Relayout on the IKM Krupuk Production Section
Authors: Prasetyo Eko Budi , Cahyana Atika Sidhi
Published: September 2021

This industry, established since 2009, is one of the industries for making raw crackers with various kinds of crackers, which is located in simo angin-angin village, sidoarjo city which produces various kinds of crackers, namely casandra crackers, rose crackers, braid crackers and made with quality flour and fuel. to oven, namely with the steam obtained from the boiler / steam boiler. This study aims to design and suggest improvements to the facility layout properly and the flow of materials smoothly, so that the material travel distance is not too long and can reduce the alternating flow of the production process material in CV Andi Jaya with the Activity Relation Chart method with the application blocplan. One indicator that can determine how good a Layout is is by comparing the total moments in the Layout. From the results of the calculation of total moment efficiency using BLOCPLAN shows better results, where there is an increase of 0.52% from the existing condition or initial layout.
Evaluation of Vertical Guided Bone Regeneration Using a Particulate Form of Experimental Bioactive Glass in a Rabbit: A Case Report with Literature Review
Authors: Melkumyan Timur V. , Kamilov Nuritdin Kh. , Daurova Fatima Yu. , Dadamova Angela D.
Published: September 2021

Background: There are a large number of different types of bone-grafting materials that are used for the regeneration of atrophied alveolar ridges in order to make dental implantation possible. However, available surgical techniques and materials for bone augmentation do not contribute to the achievement of the desired reliable results and require a search for new solutions to an existing problem. A group of synthetic osteoplastic materials based on bioactive glass (BAG) may become a matter of choice in bone tissue regeneration because of special osteogenic properties. The aim of this study was to visually and histologically evaluate the behavior of an experimental BAG in rabbit tibia bone samples, which were collected from the animal 6 weeks after filling the bone defects. Methods and Results: The observation was carried out on one outbred rabbit whose tibia bone defects were filled with an experimental osteoplastic material based on the BAG. The chemical composition of the experimental osteoplastic material included SiO2 (41%), Na2O (21%), CaO (28.5%), P2O5 (6%), CaF2 (1.5%), MgO (1%), Al2O3 (1%). For histological analysis, H&E staining of paraffin-embedded tissues was performed according to the standard technique. Light microscopy of tissue samples was performed using a Leitz HM-LUX microscope (Germany). Six weeks after filling the bone defects, a strong bond between the augmented hard tissue and rabbit tibia was recognized. Also, a dense fusion of adjacent soft tissues with a newly formed bone without signs of chronic inflammation or graft particles in granular tissue was noted. Microscopic examination of the stained sections showed the presence of mature viable BT with a uniform distribution of osteocytes. Also, residual fragments of the degraded biomaterial surrounded by the fibers of a woven bone were revealed in several slices. Conclusion: In accordance with the results of this experiment, it can be concluded that the usage of BAG related to the system SiO2(41%)-Na2O(21%)-CaO(28.5%)-P2O5(6%)-CaF2(1.5%)-MgO(1%)-Al2O3(1%) may increase the volume of bone without application of barrier membrane. However, further research involving more animals needs to be done to estimate the scientific significance of the obtained data and to evaluate the mechanical properties of augmented bone.
Evaluation of the Prognostic Value of Heart Rate Variability in Elderly Patients with Multivessel Coronary Artery Disease against the Background of Invasive and Non-Invasive Treatment
Authors: Abdullaeva Saodat Ya.. , Nikishin Aleksey G. Nikishin , Mullabaeva Gusal U. , Bekmetova Feruza M.
Published: September 2021

The purpose of this study was to evaluate the prognostic value of HRV in elderly patients with multivessel coronary artery disease (MVCAD) on the background of invasive and non-invasive treatment. Methods and Results: This study included 254 patients over age 65 with lesions of the left trunk of the left coronary artery in combination with lesions of 2 or more coronary arteries. To assess HRV, all patients underwent 24-hour Holter ECG monitoring at baseline and one year later. Depending on the treatment strategy, patients were divided into 3 groups. Group 1 consisted of 99 patients who, in addition to the standard treatment, underwent PSI (from 1 to 4 stents); Group 2 included 86 patients who, in addition to the standard treatment, underwent CABG (from 2 to 4 shunts); Group 3 included 69 patients who received only optimal drug therapy (ODT). The results have shown that a decrease in HRV is an independent predictor of complications associated with an increase in coronary insufficiency in CAD patients. Such indicators of HRV as SDNN, SDNNi, TP, VLF, and LF have a significant positive predictive value in patients undergoing ODF and/or undergoing PCI. For patients undergoing CABG, at least in the first year after surgery, HRV cannot be considered as an independent prognostic marker. For elderly patients with MVCAD, 24-hour Holter ECG monitoring with subsequent analysis of HRV is recommended to assess the recovery process and pharmacotherapy.
Electrical Activity of the Heart in the Older Adults Suffering from Chronic Kidney Disease: Ethnic and Age Characteristics
Authors: Kudrina Polina I. , Shadrina Svetlana S. , Sofronova Sargylana I. , Bogolepova Anna N. , Donskaya Ariadna A. , Borisova Natalya V. , Karelin Andrey P.
Published: September 2021

The aim of the study was to investigate the ethnic and age-related characteristics of the electrical activity of the heart in older adults with chronic kidney disease (CKD). Methods and Results: A total of 522 patients aged between 60 and 89 years with different stages of CKD were examined. For a comparative analysis, we formed 3 ethnic groups (Yakuts, Evens, and Russians) and 2 age groups (60-74 years and 75-89 years). All patients underwent ECG. The laboratory analysis included determining the blood creatinine level for further calculation of the GFR using the Cockcroft&Gault formula, followed by determining the stage of CKD. A decrease in renal function was more typical for people of 75-89 years. The results of the study confirmed the close relationship of CKD in older adults with the pathology of the cardiovascular system. Changes in the electrical activity of the heart predominated in Russians, compared to other ethnic groups, and were less pronounced in the Evens.
Genetic Predictors of Type 2 Diabetes in Yakuts
Authors: Pavlova Nadezhda I. , Diakonova Aleksandra T. , Alekseev Vladislav A. , Sydykova Lyubovy A. , Maksimova Nadezhda E. , Rudykh Zoya A. , Chertovskikh Yana V. , Sychev Dmitry A. , Dodokhov Vladimir V. , Dmitrieva Tuyara I. , Kurtanov Khariton A.
Published: September 2021

The goal of this study was to investigate the distribution of alleles and genotypes of the KCNJ11 rs5219, PPARG rs1801282, TCF7L2 rs7903146/rs12255372 SNPs in Yakuts with T2D, in comparison with other ethnic populations. Methods and Results: The study cohort consisted of 26 Yakut patients diagnosed with T2D (YKT2D). Genotyping of rs5219 (KCNJ11), rs1801282 (PPARG), rs7903146 and rs12255372 (TCF7L2) SNPs was performed by pyrosequencing using PyroMark Q48 Autoprep sequencer (QIAGEN). The genotyping of the studied group of Yakuts did not reveal statistically significant differences between control groups and YKT2D patients with respect to the polymorphic variants of the KCNJ11, PPARG, and TCF7L2 genes. The allele frequency analysis of the polymorphisms of the KCNJ11, PPARG, and TCF7L2 genes demonstrated a low frequency of the risk T-allele in the TCF7L2 (rs7903146, rs12255372) in Asian populations, compared to other human populations. We identified three haplotypes [CG (90.5%), TT (6.8%), and TG (2.7%)] in the YKT2D cohort. Also, we observed a strong LD between two SNPs (rs7903146 and rs12255372) of the TCF7L2 gene in the majority of groups, including YKT2D (D '= 1, LOD = 4.92), except for African populations, where a very weak LD (D '= 0.001-0.435, LOD = 0.0-0.73) was observed.
Association of the AGTR1 Gene A1166C (rs5186) Polymorphism with Essential Hypertension in the Indigenous Population of the Arctic
Authors: Sofronova Sargylana I. , Klimova Tatyana M. , Kirillina Marya P. , Nikolaev Vyacheslav M. , Kononova Irina V. , Kuzmina Ariana A. , Romanova Anna N.
Published: September 2021

The research objective was to study the association of the AGTR1 rs5186 SNP (the A1166C variant) with essential hypertension among indigenous people of the Arctic territory of Yakutia. Methods and Results: A total of 351 participants (224 women and 127 men) were examined, including 56 Yakuts, 34 Chukchi, 77 Yukaghirs, and 184 Evens. The Case (n=168) and Control (n=183) groups were formed. Allelic variants of the AGTR1 rs5186 SNP were tested by real-time PCR. We did not find statistically significant differences in the frequency distribution of the alleles and genotypes of the AGTR1 rs5186 SNP between the Case group and the Control group. Conclusion: The obtained data show no association of the AGTR1 A1166C polymorphism with EHT in the representatives of indigenous people of the Arctic territory of Yakutia.
C3435T Polymorphism of the ABCB1 gene in the Yakut Population
Authors: Pavlova Nadezhda I. , Diakonova Aleksandra T. , Alekseev Vladislav A. , Borisova Yigulana P. , Dodokhov Vladimir V. , Dmitrieva Tuyara I. , Kurtanov Khariton A.
Published: September 2021

Background: The ABCB1 gene is responsible for resistance to various cytotoxic drugs. The product of the ABCB1 gene, P-glycoprotein (P-gp), acts as a transmembrane pump and influences the action of many drugs. More than 40 SNPs of the ABCB1 gene that alter the expression of P-gp have been identified. The ABCB1 rs1045642 SNP, designated as C3435T (C-the wild-type allele, T-the variant allele), correlates with the activity of P-gp. The aim of our research was to study the distribution of alleles and genotypes of the ABCB1 C3435T polymorphism in Yakuts, in comparison with other human populations. Methods and Results: The studied cohort included 149 healthy Yakut volunteers (36 men and 113 women). The average age of participants was 30.67±0.06 years. The ABCB1 gene is a highly polymorphic gene; the allele frequency of the C3435T polymorphism differs widely among the studied populations. The frequency of the mutant T-allele among the Yakuts was 51% .In the studied group of Yakuts, we revealed the prevalence of the heterozygous CT genotype (75.8%). The Yakuts have a relatively low frequency of CC (10.7%) and TT (13.4%) genotypes. This preliminary study did not include the objective of proving the relationship between the ABCB1 C3435T polymorphism and addictive disorders in Yakuts. The further search for functional polymorphisms of the ABCB1 gene and associations with addictive behavior using a systematic approach on larger samples is of great practical importance.
Combined Correction of Experimental Critical Ischemia of the Lower Extremities in a Rat Model
Authors: Feyziev Elvin E. , Sukovatykh Boris S. , Belous Alexander S. , Zatolokina Maria A. , Trubnikova Elena V. , Mishina Ekaterina S.
Published: September 2021

The purpose of our research was to study the effectiveness of correcting experimental critical ischemia (CI) of the lower extremities with a combination of udenafil, simvastatin, and autologous bone marrow mononuclear cells (ABMMC). Methods and Results: The experiments were carried out on 24-month-old Wistar rats, weighing 220–250g. The animals were randomized by sex and weight. Groups were formed according to the manipulations carried out during the operations. The animals were divided into 7 groups, each with 20 animals: Group 1 included intact animals; Group 2 - falsely operated animals; Group 3 (control group) - animals with simulated CI without treatment; Group 4- animals with CI and monotherapy with udenafil (daily oral administration of 8.6mg/kg for 28 days); Group 5 - animals with CI and simvastatin monotherapy (daily oral administration of 1.71mg/kg for 28 days); Group 6 - animals with CI and monotherapy with ABMMC (parenterally, once on Day 7 after modeling CI, 50μl at 4 points and, paravasally, above the inguinal ligament in the area where the lateral artery leaves the artery enveloping the femur from the internal iliac artery; in the area of the superficial artery that bends around the iliac bone under the inguinal ligament; into the area of origin of the muscular branch of the femoral artery r. muscularis, the place of attachment of the comb and long adductor muscles of the thigh; in the upper third of the gastrocnemius muscle]); Group 7 - animals with CI and combination therapy (udenafil and simvastatin drugs were administered intragastrically 0.86 mg/kg, once a day, for 7 days) and one-time parenteral administration of ABMMC, according to the same scheme as in Group 6. On Days 21 and 28 of the experiment, the level of blood microcirculation was determined in the muscles of the rat leg; for this, laser Doppler flowmetry was used. For further morphometric assessment of the leg muscles, they were removed. Preparations for morphometric analysis were prepared according to the standard technique with Van Gieson staining, as well as H&E. Our study demonstrated the effectiveness of combination therapy with udenafil, simvastatin, and ABMMC to correct critical lower limb ischemia in rats. The severity of morphological changes on the background of this combination was minimal, compared to the findings of other study groups, and the level of blood microcirculation in the ischemic zone on Day 28 was, significantly, 1.9 times higher than in animals of the control group. The results obtained allow us to recommend the use of the investigated combination (udenafil+simvastatin+ABMMC) for the treatment of patients with critical limb ischemia, both in outpatient and inpatient practice.
Morphofunctional Rearrangement of the Fibrous Structures of the Rat Dermis under the Conditions of Implantation of 3D Scaffold Based on Polyprolactone
Authors: Mishina Ekaterina S. , Zatolokina Mariya A. , Ryazaeva Lydia M. , Pol'skoy Viktor S.
Published: September 2021

Background: the use of various scaffolds allows us to model the future fibrous framework of the newly formed regenerate, and also serves as a substrate for the settlement of the cellular component. The development of tissue engineering in regenerative medicine demands an understanding of the more specific mechanisms of the formation of the connective framework at the site of the defect. The aim of this research was to study the morphofunctional rearrangement of the fibrous structures of the rat dermis in response to the implantation of a 3D scaffold based on polyprolactone Methods and Results: The experiment was performed on 30 white male Wistar rats. The object of the study was a skin fragment together with an implantable 3D scaffold based on polyprolactone, taken on Days 3, 7 and 14 after implantation. Biomaterial with implantable scaffold was studied using light and scanning electron microscopy. The results of the study indicate that the 3D scaffold based on polyprolactone has good biocompatibility, causing a weak inflammatory reaction, and contributes to the formation of the connective tissue framework by Day 14. Conclusion: The results of the study can be used to develop new scaffolds or modify existing ones, as a "framework" for populating the cellular component and creating tissue-engineering structures.
Polymorphisms in Genes Involved in Steroidogenesis in the Development of Severe Acne
Authors: Rumyantsev Alexander  G. , Dеmina Olga M.
Published: September 2021

Background: Acne is a multifactorial disease, in the pathogenesis of which one of the leading factors is the excessive effect of androgens on the hair follicles (HFs) and sebaceous glands (SGs), along with hypersecretion of sebum, pathological follicular hyperkeratosis and an inflammatory response. The search for genotypic markers in patients with varying severity of acne is a difficult task due to the multifactorial pathogenesis and the role of trigger factors in the formation of acne. The aim of this study was to determine SNPs within 3 genes involved in steroidogenesis (MVK, ARPC1B, and CA2) in patients with severe acne. Methods and Results: The study included 70 patients (42 men and 28 women) aged between 15 and 46 years (the median age - 22.1 years). The main group (MG) included 50 patients (29 men and 21 women) with severe acne. The control group (CG) consisted of 20 apparently healthy individuals (13 men and 7 women). Molecular-genetic diagnostics was carried out by the method of high-throughput DNA sequencing (next-generation sequencing). Our study showed that severe acne is associated with 12 polymorphic loci of the MVK gene (4 SNPs in exons and 8 SNPs in introns), 7 SNPs of the ARPC1B gene (2 SNPs in exons and 5 SNPs in introns), and 9 SNPs of the CA2 gene (3 SNPs in exons and 6 SNPs in introns). Conclusion: The revealed features of the SNPs within the MVK, ARPC1B, and CA2 genes in patients with severe acne probably indicate a hereditary determination of steroidogenesis in acne.
Problems in Diagnosing Autism Spectrum Disorders in the Irkutsk Region
Authors: Tkachuk Elena A. , Rychkova Lyubov V.
Published: September 2021

The purpose of this study was to evaluate the effectiveness of methods for studying the neuropsychiatric development of children in regards to screening for the diagnosis of autism spectrum disorders (ASD) with the example of educational institutions in the Irkutsk region. Methods and Results: Two groups of children in educational institutions of the Irkutsk region regional center were studied: 187 children of preschool age (from 5 to 6 years) and 154 children studying at school (from 7 to 11 years). This study used the methods of neuropsychiatric research used by the pediatric service and the methods of screening diagnosis of autism spectrum disorders (ASD). It was shown that the level of obvious anxiety of schoolchildren (according to the scale developed by A.M. Prihozhan) was higher than in preschoolers and was 10.3±0.1% in girls and 10.6±0.2% in boys. Bad appetite was observed in 50.3±3.7% of preschool children and in 56.5±4.0% of schoolchildren. The selective appetite was observed in 16.6±2.7% of preschool children and in 7.1±2.1% of schoolchildren. The study of hyperactive traits showed that only 5.3±1.6% of parents of preschool children noted hyperactivity in their children while educators considered that 22.5±3.1% were hyperactive. The parents noted hyperactivity in 9.7±2.4% of children, and the teachers noted hyperactivity in 21.7±3.4% of children. The intellectual development of children, according to the Raven test, showed that the average IQ was observed in 47.6±3.7% preschoolers, and below-average IQ in 52.9±3.7% preschoolers. At the same time in schoolchildren, below-average IQ was found in 48.7±4.0% and average IQ in 51.3±4.0%. Conclusion: The study of the development of children’s mental processes and behavior is not informative for the early detection of ASD. Likewise, the screening methods for the detection of ASD known today and available to the pediatric service and based on the detection of social and communication disorders are poorly informative. However, if we consider ASD as a manifestation of genetic and cytogenetic pathology, we should look for screening methods in the field of genetics.
Adaptive Changes in the Psyche of Homo Sapiens during the Period of the Singularity (Part 3)
Authors: Kruglov Alexander G. , Kruglov Andrey A.
Published: September 2021

Personal constitutional and acquired predispositions form preferences in the vectors of perception of information (cultural) sentences of the environment. On these vectors, contextual factors are formed that affect the processing of incoming information, the formation of representations and images, which determine the interpretation of lexical signs. Multiplication of contexts creates metacontexts that define the boundaries of virtual reality. One of the design features of Clip thinking (ClipT) is the formation of metacontexts by external structures: network associations. The metacontexts of ClipT form a new structure of communicative experience that changes the self-identification and socialization of the subject, causing a state of dependence. ClipT has significant similarities with hieroglyphic thinking (HieT), which consists of quantized combinations: image + sense + emotion + tone. Hieroglyphics (thinking and writing) have a metacontext nature, being a formation of an ethnic scale. According to some structural parameters, KlipT and HieT are similar to the level of identity. Fundamental factors are the external genesis of metacontexts; the imagery of thinking; elimination of causal relationships and abstraction; visual "receiving-transmission" of the sense of the perceptual image, including the graphic image; rigidity of mental and behavioral structures; embedded experience; etc. The integral structure of a legitimizing nature (HieT + writing + language + metacontext continuum (ethnocultural matrix)) forms a parametrically conjugated social structure derived from it. A monolithic socio-cultural conglomeration with a self-reproduction mechanism is created. We believe this principle is universal, with the possibility of extrapolation to any socio-cultural structure. Currently, the HS population is in a state of forming a universal cultural matrix with the potential to replace ethnocultural matrices. ClipT—the new operating system of the psyche—defines and unifies the transformation of ethnocultural matrices with the vector of universalization. The significant similarity of hieroglyphics with the parameters of ClipT contains the extrapolation potential of modeling (sociological and mathematical) the expected ethnic/universal dynamics of conglomeration: ClipT (psyche as a whole) and congruent social construction. The identity points of ClipT and HieT can find application in the constructions and artificial intelligence learning, as reference points of operating systems of thinking and language, in the structure of which there are no algorithms for cause-effect relationships, analysis, feedback, abstract thinking, and classification structures dominate.
Phosphorylation and Fragmentation of the Cardiac Troponin T: Mechanisms, Role in Pathophysiology and Laboratory Diagnosis
Authors: Chaulin Aleksey M.
Published: September 2021

Cardiac troponin T (cTnT), a protein essential for calcium-regulated, myofibrillar ATPase activity, is extremely sensitive to the action of a significant number of intra- and extracellular enzymes, the action of which causes post-translational modifications (PTMs) of amino acid structure and functioning cTnT. PTMs of cTnT may play important roles in the regulation of cardiac contractility. The vast majority of cTnT modifications involve the phosphorylation by a variety of Ser/Thr kinases, including PKC. At the same time, the activity of cTnT phosphorylation can change under physiological conditions and in some CVDs, including HF, AMI, and arrhythmias. Along with cTnT phosphorylation, cTnT fragmentation occurs, the activity of which can also change. This article discusses the mechanisms of cTnT phosphorylation and fragmentation, discusses the important role of these processes in the pathophysiology and laboratory diagnosis of some CVD, and notes promising directions for further research.