bluuebunny/crossref_metadata_2025 huggingface dataset:
Gestión administrativa y la calidad de servicio en el área de retenciones de TC del Banco Scotiabank, sede Arenales, 2021
Authors:   , Alcas Abanto Aurora Margarita , Llacqua Cotos Kelly Katherine
Published: February 2023

Dentro del marco de este estudio se tomó como referencia las variables gestión administrativa y calidad de servicio en el área de retenciones de tarjetas de crédito del Banco Scotiabank en la sede Arenales (Lima-Perú) en el 2021. El propósito general consideró determinar el grado de relación entre la gestión administrativa y la calidad de servicio. Con respecto a la metodología, cuantitativo fue el enfoque, básico el tipo; además el diseño fue descriptivo correlacional. La población fue censal; por lo tanto, la muestra estuvo compuesta por 50 participantes. Por su parte, el procedimiento para el levantamiento de los datos se materializó por medio de la encuesta y el instrumento fue el cuestionario, elaborado por las investigadoras. Del mismo modo, para el tratamiento estadístico se trabajó con el programa SPSS ver 26. En síntesis, las conclusiones halladas determinaron un factor de correlación positivo medio (Rho=0,583) y un valor de significancia (sig.=0,00). Es decir, la hipótesis alterna fue verdadera, donde asegura la relación significativa entre la gestión administrativa y la calidad de servicio.
Hydraulic sand test and analysis based on sound emission data and fracture surface
Authors: Zhang Shuhui
Published: February 2023

Due to the extremely low permeability of shale reservoirs, large-scale reservoir fracturing is required. Hydraulic fracturing is one of the most important technologies in shale gas exploration and development. In this paper, the acoustic emission energy and the number of location and fracture surface morphology of specimens before and after fracture are studied through hydraulic sand fracturing test. The test results show that: (1) the energy ratio obtained during hydraulic fracturing without proppant is the smallest, and increasing the confining pressure, as well as reducing the displacement and viscosity of the fracturing fluid will cause the energy ratio to decrease. From the perspective of acoustic emission energy, the proppant It plays an important role in the generation of fractures during hydraulic sand fracturing; (2) when the confining pressure increases, the number of shale specimens before and after rupture is the largest, but the total number of locating events is smaller than when the sanding ratio is increased; when there is no proppant hydraulic fracturing, the number of specimens before and after the rupture is the largest And the total number reached the minimum, indicating that the proppant can play an important role in the hydraulic sand fracturing test; (3) the sand is relatively large, the specific surface and standard deviation both reach the maximum, indicating that the fracture surface roughness is the largest under this test condition, and the fracturing effect is the best, but the specific surface and standard deviation are the minimum when fracturing without proppant, Indicating that the fracture surface fracturing effect is the worst at this time.
Utilization of reeds to sequester Ni and/or Cu from wastewater and to produce valuable products
Authors: vudang Ken , Duran Valeria , Brdecka Michael , Seigerroth Julia , Jang Ben , guo lin
Published: February 2023

This project evaluated the potential of common reed to uptake Ni and Cu from wastewater and then investigated the feasibility of converting the resulted biomass to valuable products. Common reeds were grown in 25, 50, or 75 ppm Ni and/or Cu solutions for 4 weeks to evaluate its potential to uptake metals followed by converting the contaminated biomass to valuable products by hydrothermal conversion (HTC). The higher concentration of metals in solution, the more metals accumulated in plants. The amounts of metals accumulated in different parts of reeds were root > stem > rhizome ~ leave. Roots and stems treated with binary metals contained less Ni/Cu than those cultured in single metal. The yields of bio-oils and hydrochars from reeds treated with different levels of metals were similar. About 6%-10% of bio-oil, 50%-60% of hydrochars were produced. The results suggest that metal accumulated in reeds does not affect the utilization of contaminated reeds via HTC. About 0.1–1.5% of Cu and/or Ni were transmitted from contaminated reeds to bio-oils after HTC at 250°C.
Systematic pan-cancer analysis identifies 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) as a biomarker of tumor invasion and metastasis, immunity, and prognosis
Authors: Liu Dandan , Liu Yingying , Zhang Chaoyang , Zhang Jingting , Xu Guo-Tong
Published: February 2023

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) is a potent regulator of glycolysis in tumor cells, and high PFKFB3 expression is significantly associated with the invasion and metastasis of several tumors. However, there are no comprehensive reports on whether PFKFB3 promotes tumor invasion and its mechanism in different cancer types. In addition, there are no systematic reports on the effect of PFKFB3 on the stemness and immune infiltration ability of different tumors and on the survival rate of patients. Herein, we conducted a pan-cancer analysis of PFKFB3 with the aim of exploring the key cellular and molecular mechanisms regulating the pathogenesis and progression of human cancers, and propose potential strategies for the prevention and treatment of cancer by targeting PFKFB3. Using bioinformatics analysis and integrative exploration from the Gene Expression Omnibus (GEO) and Cancer Genome Atlas (TCGA) databases, the differential expression of PFKFB3 or phosphorylated PFKFB3 and its correlation with tumor staging and stemness, survival prognosis, and immune invasion were extensively analyzed. The analysis showed differential expression of PFKFB3 in normal tissues and in various cancers. Increased PFKFB3 expression is positively correlated with the invasive ability and immune infiltration of 31 cancers and significantly affects the staging, stemness, prognosis, and survival rate of several cancers. Alterations in phosphorylated PFKFB3 and RNA modifications are also involved in the development and progression of various cancers. PFKFB3 is involved in multiple protein interactions and has complex molecular functions, such as ATP/ADP metabolic and glycolytic processes. Furthermore, PFKFB3 has a high mutation frequency, especially amplification, in multiple tumors. These findings highlight the significance of PFKFB3 in cancer progression, which might serve as a surrogate pan-cancer biomarker to predict the progression and outcome of cancers, as well as the invasion and immune infiltration of different cancers. Ethical compliance: This study did not involve any patient or animal samples and was approved by the academic committee of Tongji University.
An Improved Deep Neural Network Based on Combination of GRU and Auto Encoder for Sentiment Analysis
Authors: Zulqarnain Muhammad , Alsaedi Ahmed Khalaf Zager , Sheikh Rubab , Javid Irfan , Ahmad Maqsood , Ullah Ubaid
Published: February 2023

Sentiment analysis is a particularly common task for determining user thoughts and has been widely used in Natural Language Processing (NLP) applications. Gated Recurrent Unit (GRU) was already effectively integrated into the NLP process with comparatively excellent results. GRU networks outperform traditional recurrent neural networks in sequential learning tasks and solve gradient vanishing and explosion limitation of RNNs. In this paper, a novel approach as known Normalize Auto-Encoded GRU (NAE-GRU) was proposed, in order to reduce dimensionality of data through an Auto-Encoder and enhance the performance of the proposed approach by using batch normalization. Empirically, we demonstrate that the proposed model, with minor hyperparameters modification, and statistic vectors optimization, achieves outstanding sentiment classification performance on benchmark datasets. The developed NAE-GRU approach outperforms than other different traditional methods in terms of accuracy and convergence rate. The experimental results have showed that the developed approach accomplished excellent performance than existing approaches on four benchmark datasets included, Amazon review, Yelp review, IMDB and SSTb. The experimental results have showed that the developed approach is proficient to reduce the loss function, and capture long-term relationships with an effective design that achieved excellent results as compared state-of-the-art methods.
Analysis and prediction of surface topography characteristics and influence factors of tool passive vibration in milling process
Authors: Zhang Wei , Su Peibin , Zhang Lei , Bai Fengsong
Published: February 2023

The surface topography of the work-piece after machining has an important influence on the service performance, and the passive vibration of the tool will occur due to the influence of the milling force during the machining process. This paper focuses on the influence of the milling parameters and the passive vibration of the tool on the formation process of the surface topography. Firstly, the forming mechanism of the surface topography when the tool is passively vibrated is studied, and the cutting edge motion trajectory model considering the milling parameters and the tool passive vibration is established; The influence of milling parameters on surface topography with or without tool passive vibration is analyzed through experiments and simulations; The prediction models of maximum height Sz and three-dimensional arithmetic mean height Sa of surface topography are established by least squares support vector machine. The improved PSO algorithm is used to search the optimal solution of the kernel width coefficient and regularization parameters in the least squares support vector machine, and the program of the improved PSO-LSSVM prediction model is compiled. The prediction model proposed by error verification can provide a theoretical basis for the selection of actual milling processing parameters.
The Effect of Cytotoxic CD8+ T-Cells Secretory Proteins on Hypoxic Pancreatic Cancer Cells
Authors: Abdo Eiman , Ismail Mohammad A. , Hadidi Sabal Al , Al-Mrahleh Mairvat , Zihlif Malik , Ababneh Nidaa A
Published: February 2023

Background: Pancreatic cancer is a highly progressive cancer with limited effective treatment. The tumor microenvironment in pancreatic cancer is aggressive and severely hypoxic with a high immunosuppressive effect. This has raised the attention to understanding the pancreatic cancer microenvironment and the interaction between pancreatic cancer cells and cytotoxic T-cells.
Objective: This study aimed to investigate the crosstalk between hypoxic pancreatic cancer cells (PANC-1) and cytotoxic CD8+ T-cells.
Methods: Pancreatic cancer cells (PANC-1) were exposed to 20 cycles of chronic hypoxic conditions for 72 hours followed by a re-oxygenation period for 24 hours. On cycles 10 and 20, PANC1 hypoxic conditioned media (CM) were harvested and the hypoxic PANC-1 cells were co-cultured with the activated cytotoxic CD8+ T-cells or with CD8+ T-cells CM. CD8+ T-cells CM was collected after 5 days of cell activation using anti-CD3/CD28 antibodies and interleukin-2 (IL-2). On the other hand, CD8+ T-cells were activated for 72 hours, and then cultured with the hypoxic PANC-1 CM.
Results: Hypoxic PANC-1 cells showed a significant increase in resistance to the lytic effect of either CD8+ T-cells coculture or CD8+ T-cells CM treatment when compared with normoxic PANC-1 cells. A significant decrease in TNF-α and IFN-γ levels was also detected. Additionally, a significant increase in IL-6, P53 and TNF-α gene expression levels was observed in PANC-1 cells treated with CD8+ T-cells CM. Moreover, IL-6 gene expression level showed a significant difference between hypoxic and normoxic PANC-1 cells. Furthermore, CD8+ T-cells proliferation and cytokines production were significantly higher in cells cultured with PANC-1 CM. However, no significant differences were observed after treatment with either hypoxic or normoxic PANC-1 CM.
Conclusion: Hypoxia influences PANC-1 cells' sensitivity to cytotoxic CD8+ T-cells. Several mechanisms of PANC-1 resistance were detected in this study; an increase in IL-6 production and a depletion in TNF-α and IFN-γ levels when co-cultured with PANC-1 cells. In treated PANC-1 cells, an increase in TP53 and TNF-α gene expression were observed. IL-6 activation is one of the mechanisms that may increase the resistance to CD8+ T-cells in hypoxic PANC-1 cells.
Insomnia plays a key role in mental health problems of COVID-19 patients in Square Cabin Hospitals: a network analysis
Authors: Zhang Tian , Li Yijun , Sun Jingjing , Yu Jing , He Wei , Wu Lin , Lin Xinxin , Wang Lingling , Peng Xiyuan , Mao Li , Peng Jiaxi , Fang Peng
Published: February 2023

Background: While the emergence of the Coronavirus Disease 2019 (COVID-19) has greatly affected the mental health and wellbeing of people everywhere, the core symptoms of common syndromes of psychological distress remain unclear, especially among COVID-19 patients who have been quarantined in Square Cabin Hospitals. Using the method of network analysis, the current study explored the relations between stress, depression, anxiety and insomnia symptoms among COVID-19 patients in Shanghai Four-Leaf Clover Cabin Hospital.
Method: The study used an online survey to gauge the mental health issues of COVID-19 patients at Shanghai Four-Leaf Clover Cabin Hospital from April 18 to May 19, 2022. The Perceived Stress Scale, Patient Health Questionnaire, Generalized Anxiety Disorder Scale, and Insomnia Severity Index were used to assess perceived stress symptoms, depressive symptoms, anxiety symptoms, and insomnia symptoms, respectively. The central symptoms and bridge symptoms were determined using a regularized partial correlation network.
Results: A total of 1014 patients was included in this study. Overall, 61.5%, 49.2% , and 56.1% of all patients reported symptoms of depressive, anxiety, and insomnia, respectively. “Distress caused by the sleep difficulties” had the highest expected influence, followed by “Excessive worry”, “Severity of sleep onset”, “Overburden”, “Uncontrollable worry”, “Trouble relaxing”, “Restlessness”. Seven bridge symptoms were identified: “Sad Mood”, “Severity of sleep onset”, “Sleep”, “Motor”, “Feeling afraid”, “Nervousness”, “Appetite”. In addition, significant differences in network global strength were found between women and men.
Conclusions: Psychological distress was prevalent among COVID-19 patients and insomnia symptoms played a key role in the network of psychological distress symptoms. As a result, specific insomnia symptoms should be closely monitored as potential intervention targets in alleviating or preventing common, frequently co-occurring psychiatric syndromes.
Nano-kinematic indicators (Gulf of Aqaba, Saudi Arabia)
Authors: Genovese Alessandro , Fedorik Jakub , Afifi Abdulkader , Bernhard Grasemann
Published: February 2023

Strain and kinematic indicators are frequently investigated to assess the type, magnitude, and orientation of deformation fields. Typically, their analysis ranges from the outcrop to the thin section scale. The present study characterizes the structural and mineralogical development of a phyllosilicate-rich fault gouge from the core of a major strike-slip fault on the eastern margin of the Gulf of Aqaba. We used transmission electron microscopy and focused ion beam techniques to explore shear deformation structures down to the nanoscale. The fault gouge was differentiated at the micron-submicron scale into chlorite-rich and illite-rich bands. The observed nano-kinematic shear sense indicators were SC/SCC’ fabrics in phyllosilicate bands, inclined axial planes in kink-folded illite and chlorite plates, platy hematite clasts inclined against the shear direction, and a σ-type clast system with recrystallized material in strain shadow wings around the clasts. These indicators provide a consistent set of criteria to determine shear sense in phyllosilicate-rich fault gouges.
Genetic polymorphism of the growth hormone gene and their effects on growth metrics in the Assam Hill and Sirohi goats under subtropical climatic condition
Authors: Kumar Rakesh , Govindasamy Kadirvel , Khargharia Gautam , Lyngdoh Erica , Singh Mahak , Das Meena , Singh Vinay , Mishra Vinay
Published: February 2023

Growth hormone (GH) is a gene that regulates milk production, reproduction, and growth in animals. Therefore, the purpose of this study was to identify GH gene variations and their associations with growth metrics in Sirohi and Assam hill goats. We identified two polymorphic loci (GH1-HaeIII & GH2-HaeIII) which were connected to growth attributes in 161 animals of Assam hill (n = 114) and Sirohi (n = 47) goats using the methods of PCR-RFLP. According to PCR-RFLP results, the GH gene's GH1-HaeIII locus contains two alleles (A and B) and two genotypes (AA and AB), whereas the GH2-HaeIII locus contains two alleles (C and D) and two genotypes (CC and CD). In the two goat breeds, the allele frequencies of A and B were 0.36 − 0.28 and 0.64–0.72, respectively, while the frequencies of alleles C and D were 0.71 − 0.70 and 0.29–0.30. The polymorphic loci were demonstrated to be in a state of Hardy-Weinberg disequilibrium in both breeds of goats (P < 0.05). The growth characteristics of the Assam hill goat breed were shown to be correlated with GH gene variations. In both breeds, AB and CD genotypes displayed slightly higher values in most of the morphometric traits compared to AA and CC genotypes; however, only in Assam hill goats body length and chest girth were positively significant (P < 0.05). Hence, the AB and CD genotypes have been proposed as potential markers for improved growth parameters in the breeds that were tested. Therefore, we came to the view that the two variant loci of the GH gene will utilize as marker assisted selection for enhancing growth attributes in goats; however, more research with a bigger sample size is necessary to verify the validity of this conclusion.
Left versus right destroyed lung pneumonectomy:short-term and long-term prognosis and risk factors
Authors: Li YunSong , Wang Heng , Wang Chunmao , Zhang Li , Wang Yuxuan , Bai Zhexin , Yan Dongjie , Liu Fangchao , Ruan Hongyun
Published: February 2023

Aim:
To compare and analyze postoperative short-term and long-term destroyed lung (DL) disease prognosis for patients undergoing left versus right pneumonectomy and to explore related risk factors.
Methods:
Retrospective analysis was conducted of clinical data obtained for 128 DL patients who underwent pneumonectomy in the thoracic surgery department of Beijing Chest Hospital from November 2001 to May 2022. Based on lesion site, cases were divided into two groups: a left pneumonectomy group (104 cases) and right pneumonectomy group (24 cases). Then, postoperative short-term and long-term DL disease clinical features and prognostic factors were analyzed and compared.
Results:
As compared with the left pneumonectomy group, the right pneumonectomy group experienced greater rates of preoperative diabetes and chronic pulmonary aspergillosis (CPA) rates and greater rates of intraoperative blood loss and postoperative respiratory failure. Right pneumonectomy group rates of rehospitalization, tuberculosis recurrence, BPF and empyema were higher than corresponding left pneumonectomy group rates. Results of binary logistic regression analysis revealed that CPA (OR: 5.234, 95% CI: 1.768-15.498) was a key risk factor for secondary respiratory failure, while residual cavity reinfection (OR: 4.402, 95% CI: 1.063-18.241) was a key risk factor for rehospitalization and right pneumonectomy was a key risk factor for tuberculosis recurrence (OR: 4.443, 95% CI: 1.241-15.907) and BPF/thoracic infection (OR: 6.713, 95% CI: 1.212-37.198).
Conclusion:
Patients undergoing right pneumonectomy experience poorer outcomes with regard to short-term postoperative safety and long-term prognosis as compared to left pneumonectomy patients. Furthermore, surgeons should exercise extreme caution when performing pneumonectomies on DL patients with CPA, who are most likely to experience residual cavity reinfection, in order to prevent this postoperative complication.
Evaluation of implementation outcomes of an integrated group postpartum and well-child care model at clinics in Malawi
Authors: Gresh Ashley , Mambulasa Janet , Ngutwa Nellie , Chirwa Ellen , Kapito Esnath , Perrin Nancy , Warren Nicole , Glass Nancy , Patil Crystal L.
Published: February 2023

Background
Persistently elevated rates of maternal and infant mortality and morbidities in Malawi indicate the need for increased quality of maternal and well-child care services. The first-year postpartum sets the stage for long-term health for the childbearing parent and infant. Integrated group postpartum and well-child care may improve maternal and infant health outcomes. The purpose of this study was to examine implementation outcomes for this model of care.
Methods
We used mixed methods to examine implementation outcomes of integrated group postpartum and well-child care. We piloted sessions at three clinics in Blantyre District, Malawi. During each session we evaluated fidelity using a structured observation checklist. At the end of each session, we administered three surveys to health care workers and women participants, the Acceptability of Intervention Measure, the Intervention Appropriateness Measure, and the Feasibility of Intervention Measure. Focus groups were conducted to gain greater understanding of people’s experience with and evaluation of the model.
Results
Forty-one women with their infants participated in group sessions. Nineteen health care workers across the three clinics co-facilitated group sessions, 9 midwives and 10 health surveillance assistants. Each of the 6 sessions was tested once at each clinic for a total of 18 pilot sessions. Both women and health care workers reported group postpartum and well-child care was highly acceptable, appropriate, and feasible across clinics. Fidelity to the group care model was high. During each session as part of structured observation the research team noted common health issues, the most common one among women was high blood pressure and among infants was flu-like symptoms. The most common services received within the group space was family planning and infant vaccinations. Women reported gaining knowledge from health promotion group discussions and activities. There were some challenges implementing group sessions.
Conclusion
We found that clinics in Blantyre District, Malawi were able to implement group postpartum and well-child care with fidelity and that it was highly acceptable, appropriate, and feasible to women and health care workers. Due to these promising results, we recommend future research examine the effectiveness of the model on maternal and child health outcomes.
rGO-ZnO nanowire deposited filamentous seaweed nanofibrous cellulose for paper supercapacitor
Authors: Bhutiya Priyank . L. , Kapdaia Rahul , Tripathi Brijesh , Sanjaliya Yash , Rasheed M. Abdul , Rao P. L.S. , Hasan S. Zaheer
Published: February 2023

Nanosized architectural (A Spider’s web) structure of cellulose
(Iα ) was extracted from green seaweed Chaetomorpha
antennina by using bleaching treatment. Further, reduced
graphene oxide (rGO) and zinc oxide (ZnO) nanowire deposited over
seaweed cellulose by using simple hydrothermal method. Simple press
method was used to prepare rGO-ZnO seaweed cellulose nanocomposite
for paper supercapacitor. This rGO-ZnO seaweed cellulose paper
anode material was characterized by using various analytical
techniques such as FT-IR, SEM, TGA, XRD and tensile test. XRD
peaks reveals that when graphene oxide powder mixed with seaweed
cellulose, it was reduced and gave Xrd peak of reduced Graphene
oxide (rGO). This paper supercapacitors were tested in CV, GCD and
EIS. From GCD, the specific energy density of ZnO- cellulose
paper device is found to be 0.00066 Wh/kg whereas, for rGO-ZnO
cellulose paper device have greater energy density of 5.21
Wh/kg. From EIS, the series resistance of ZnO- cellulose is found
to be 326 Ω and for ZnO-rGO -cellulose is 2.16 Ω. This marine
resources based rGO-ZnO seaweed cellulose paper supercapacitor have
application in various energy storage domains including electric
vehicles and electronic industries because it is bio-degradable,
cost effective, thinnest, safe to use as well as high
performance.
Phylogenetic relationships of Prunus (Rosaceae) distributed in North East India: insights from the ribosomal internal transcribed spacer sequence (ITS)
Authors: Mekrini Jennifer N. , Thongam Biseshwori
Published: February 2023

Background
Prunus L. is a large genus including trees and shrubs such as apricot, almond, peaches, plum and nectarines belonging to family Rosaceae. The phylogenetic analysis using ITS sequence was conducted to understand the evolutionary relationship between the Prunus sp. of North East India.
Methods and results
The present study focuses on the 32 accessions, comprising of six voucher specimen of Prunus cerasoides, one voucher specimen of Prunus campanulata, one voucher specimen of Prunus napaulensis, one voucher specimen of Prunus domestica and other twenty two voucher specimen of Prunuscomprising 5 subgenera according to Rehder (1940) and one voucher specimen of Pyrus communis (outgroup) archived from GenBank. The molecular marker (ITS) was utilized in the present study to understand the phylogenetic relationship. Various molecular markers are utilized in assessing the phylogenetic relationship and genetic diversity of Prunus. The reconstruction using the ITS region is to evaluate the evolutionary relationship of our species collected from North East region and to reflect on the relatedness of the taxa based on the ML and Bayesian method. The result of phylogenetic analyses based on ITS sequence clustered two major groups comprising of the Cerasus-Padus-Laurocerasus complex and the Prunus-Amygdalus complex.
Conclusion
The ITS sequences of the Prunus species from the present investigation would be helpful in the future study as it provides access to the sequences from the region of North East India as there is no phylogenetic study conducted till date.
Heavy rainfall events in the state of Acre, western Amazonia
Authors: Moreira José Genivaldo do Vale , de Almeida Maila Pereira , Silva Sonaira Souza da , Duarte Sergio Nascimento , Mendonça Fernando Campos
Published: February 2023

Currently, humanity is unable to prevent extreme hydrological events from occurring. However, the use of appropriate tools to estimate the probability associated with different hydrological variables, such as heavy rainfall, can help mitigate social and environmental damage. The objective of this study was to verify the hypothesis that the maximum rainfall records (over different intervals of duration) from the three main rainfall stations in the state of Acre fit the Gumbel distribution. Additionally, the periods of intense rainfall were evaluated based on the association between rainfall duration and return period. Using the maximum likelihood method, and once the baseline stationarity assumption established by the Mann-Kendall test was met, the relevant equations were fitted to the classical Gumbel model. Adherence to the model was assessed using the Anderson‒Darling test. The fitted distributions allowed the estimation of rainfall intensity for different intervals of duration and return periods for the cities of Cruzeiro do Sul, Tarauacá and Rio Branco. These results are relevant for the implementation of plans aimed at strengthening the water security in the state of Acre.
Induction of antitumor immunity by CCR2 transduced CD8+TILs in mouse lung carcinoma
Authors: Wan Yi , Fan TianYu , Wang Xin , Wang Bin , Zhang Bei , Zhang Li
Published: February 2023

Despite recent therapeutic progress in lung carcinoma, the overall survival still remains poor. Therefore, exploration of novel therapeutic strategies remains a potential objective. Chemokine was first discovered as chemotactic factor during inflammation. The CCL2-CCR2 axis is one of the major chemokine signaling pathways, and has multiple functions in tumor microenvironment, such as tumor cell proliferation and invasiveness, angiogenesis, and recruitment of immune cells. In our previous study, CCR2 was found with the distinct expression modes from other cancer types such as breast cancer, pancreatic ductal adenocarcinoma, or prostate cancer: The expression was positive correlation with the prognosis and negative correlation with T and N classifications of lung carcinoma, and was higher in antitumor immune cells of CD8 + TILs, M1 macrophages, and active/resting CD4 + T memory cells compared other tumor-promoting immune cells. In the present study, we verified CCR2 expression in human and mouse lung carcinoma, and the antitumor functions were studied in mouse lung carcinoma isolated CD8 + TILs. We found that retroviral CCR2 transduction led to the activation in CD8 + TILs, improvement of its migration to the tumor microenvironment, promotion of the early apoptosis of tumor cells, and inhibition of the proliferation of tumor cells. All the data implied the antitumor function of CD8 + TILs with up-regulated CCR2 in lung carcinoma may be provide novel targeting therapeutic strategy in the near future.
Correlation between CBR and DCPT studies of different soils
Authors: ponduri Samatha chowdary , krosuri Siva Prasada rao , ponduri Siddharth chowdary
Published: February 2023

California bearing ratio test commonly referred as CBR test is an important test to be conducted for design of any pavement. However, since CBR can’t be easily determined in the field, the need for prediction of CBR from other soil support tests such as Dynamic cone penetrometer is a valuable alternative. In the present study dynamic cone penetrometer tests from the field were correlated with the CBR studies conducted in the laboratory. Unique models were developed for each classification of soil with good coefficient of determination (R2) and low standard error of estimate. The data thus combined gave a correlation between CBR and D that compare very well with studies from various other data.
Spontaneous pneumomediastinum in a COVID-19 patient after renal transplantation: a case report and literature review
Authors: Li Shaobo , Sikong Yinhe , Li Jianjun , Ding Meiling
Published: February 2023

Background: Spontaneous pneumomediastinum is a rare complication in COVID-19. Severe pneumonia complicated with pneumomediastinum after renal transplantation is rarely reported. Here we report a case of pneumomediastinum before invasive mechanical ventilation (IMV) in a COVID-19 patient with long-term immunosuppressive therapy after renal transplantation.
Case presentation: A 57-year-old man was admitted to our center with the main complaint of “fever and dyspnea for 5 days”. His past medical history was notable for renal transplantation and with long-term immunosuppressive and anti-rejection therapy. We made the diagnosis as COVID-19 pneumonia (severe type). We managed the patient with high flow nasal cannulae (HFNC), oral dexamethasone, broad-spectrum antibiotic, prophylactic anticoagulation, and anti-viral therapy with reduced dose of azvudine due to severe renal insufficiency. During hospitalization, the patient suffered from several times of aggravation of dyspnea. First bedside chest X-ray showed suspicious pneumomediastinum and subcutaneous emphysema, and the gas in the mediastinum gradually increased. The patient’s status deteriorated rapidly, we performed urgent trachea intubation and mechanical ventilation with low tidal volume lung-protective model and performed mediastinal decompression by suprasternal drainage. Despite our active rescue efforts, the patient still died of severe infection and multiple organ failure.
Conclusions: In conclusion, we are the first to report spontaneous pneumomediastinum in a renal transplant recipient with severe COVID-19 pneumonia. This case reminds us that pneumomediastinum is a severe complication and a poor prognostic factor of COVID-19 pneumonia, especially when it occurred without positive pressure ventilation and in immunocompromised patients.
A cytokine-responsive promoter is required for distal enhancer function mediating the hundreds-fold increase in milk protein gene expression during lactation
Authors: Hennighausen Lothar , Lee Hye Kyung , Liu Chengyu
Published: February 2023

During lactation, specialized cells in the mammary gland produce milk to nourish the young. Milk protein genes are controlled by distal enhancers activating expression several hundred-fold during lactation. However, the role of promoter elements is not understood. We addressed this issue using the Csn2 gene, which accounts for 10% of mRNA in mammary tissue. We identified STAT5 and other mammary transcription factors binding to three distal candidate enhancers and a cytokine-response promoter element. While deletion of the enhancers or the introduction of an inactivating mutation in a single promoter element had a marginable effect, their combined loss led to a 99.99% reduction of Csn2 expression. Our findings reveal the essential role of a promoter element in the exceptional activation of a milk protein gene and highlight the importance of analyzing regulatory elements in their native genomic context to fully understand the multifaceted functions of enhancer clusters and promoters.
A case of brucellosis spondylitis complicated by neurobrucellosis-a Case Report
Authors: Zhu Shu-lai , Pan Xin-xin , Liu Ying , Peng Yu-hua
Published: February 2023

Introduction: We report a case of neurobrucellosis following brucellosis spondylitis. To date ,this is the first description of a patient suffering neurobrucellosis following brucellosis spondylitis.
Case Presentation: The patient was an elderly men with a history of feeding sheep and cows. The common symptoms included cervicoldynia and progressive paralysis of limbs. According to the results of blood bacterial culture, brucella agglutination test, CSF culture, tissue culture and Next-generation sequencing, the patient was considered meningitis following brucellosis spondylitis. According to the progressive weakness of the limbs, the patient received discectomy and anti-brucella therapy, the clinical symptoms gradually improved. But remained with mild paralysis of limbs although by 1-month rehabilitative training.
Conclusion: When central nervous system presented with patient of brucellosis spondylitis, we should be aware of neurobrucellosis.
Endophenotype Network-based Approach reveals the Pharmacological Mechanism of Osthole against D-Galactose Induced Cognitive Disorder in Rats
Authors: Fu Xiaomei , Wang Xue , Lai Yiyi , Cai Chuipu , Liao Yanfang , Dai Zhao , Xu Huilin , Fang Shuhuan , Fang Jiansong
Published: February 2023

Background:Osthole, a natural coumarin, found in many medicinal plants. Previous studies have shown its neuroprotective effects, whereas the effect and fundamental mechanism of Osthole for alleviating AD-associated dysmnesia is still not fully clear.Purpose:This study aimed to examine the neuroprotection of Osthole against cognitive impairment in the D-galactose-induced rats and its pharmacological mechanism.Method:The rat was constructed by subcutaneous injection of D-galactose at a dose of 150 mg/kg/day for 56 days as a model. The effect of Osthole on cognitive impairment was evaluated by behavior and biochemical analysis. Subsequently, a combination ofin silicoprediction and experimental validation was performed todetermine the underlying mechanisms of Osthole against Alzheimer's disease, while to verify the network-based predictions, western blot, Nissl staining, and immunofluorescence were applied.Result:Osthole could improve memory dysfunction induced by D-galactose in Sprague Dawley male rat. Endophenotype-based network approach highlight several AD-related pathological processes that may be regulated by Osthole, including neuronal apoptosis, neuroinflammationand endoplasmic reticulum stress. Among them, the proapoptotic markers (Bax), antiapoptotic protein (Bcl-2), moreover, the microgliosis (Iba-1), Astrocytosis (GFAP), and inflammatory cytokines (TNF-α1), levels of ER stress-associated proteins (BIP, p-PERK/PERK, Caspase12, CHOP and XBP1s) were evaluated in both hippocampus and cortex. And the results indicated that Osthole significantly ameliorated neuronal apoptosis, neuroinflammation and ER stress in D-galactose induced rats.Conclusion:This study explored the pharmacological mechanism of Osthole against D-galactose induced memory impairment and identified Osthole as a potential anti-AD drug candidate targeting multiple signaling pathways by endophenotype network-based.
Universal Shape Replication Via Self-Assembly With Signal-Passing Tiles
Authors: Alseth Andrew , Hader Daniel , Patitz Matthew J.
Published: February 2023

In this paper, we investigate shape-assembling power of a tile-based model of self-assembly called the Signal-Passing Tile Assembly Model (STAM). In this model, the glues that bind tiles together can be turned on and off by the binding actions of other glues via "signals". Specifically, the problem we investigate is "shape replication" wherein, given a set of input assemblies of arbitrary shape, a system must construct an arbitrary number of assemblies with the same shapes and, with the exception of size-bounded junk assemblies that result from the process, no others. We provide the first fully universal shape replication result, namely a single tile set capable of performing shape replication on arbitrary sets of any 3-dimensional shapes without requiring any scaling or pre-encoded information in the input assemblies. Our result requires the input assemblies to be composed of signal-passing tiles whose glues can be deactivated to allow deconstruction of those assemblies, which we also prove is necessary by showing that there are shapes whose geometry cannot be replicated without deconstruction. Additionally, we modularize our construction to create systems capable of creating binary encodings of arbitrary shapes, and building arbitrary shapes from their encodings. Because the STAM is capable of universal computation, this then allows for arbitrary programs to be run within an STAM system, using the shape encodings as input, so that any computable transformation can be performed on the shapes. This is the full version, containing all construction and proof details, of a previously published extended abstract version that had most details omitted.
Efficient Removal of Methylene Blue by Micro-/Mesoporous Heteroatom-doped Biochar Derived from Waste Bovine Horn
Authors: Peng Qifan , Du Zhen , Ma Chaoqiang , Guan Yuepeng , Feng Zhenxing , Huang Yaqin
Published: February 2023

Efficient removal of dyes from wastewater has become an urgent issue due to the rapid industrial development and increasing health concern. Here, micro-/mesoporous heteroatom-doped biochars (BHBs) were prepared from waste bovine horn by controlled pyrolysis. The BHB that had undergone a pre-oxidation reaction before pyrolysis has honeycomb-like porous structure with high specific surface area of 2447.41 m2 g− 1 and the pore size of lower than 5 nm. The tunable porous structure of the biochar could shorten the travel distance of dye molecules from external surface of biochar to inner surface of pores. And the heteroatoms could enhance the interactions, including electrostatic interaction and hydrogen bonding interaction between carbon surface and methylene blue molecules. For adsorption of methylene blue, the BHB exhibited high adsorption capacity of 1720.92 mg g− 1 at 30 ℃ and could reach a removal efficiency of near 100% in 0.5 h. The excellent adsorption performance of BHB for dye makes it a potential adsorbent for wastewater purification.
Factors associated with risk sexual behaviors among sexually active college students in Sichuan, China: a cross- sectional survey
Authors: Dai Yingxue , Li Yajie , Zhou Dinglun , Zhang Jianxin
Published: February 2023

Background
Males account for a significant share of new infections among young people in China. This study aimed to identify the factors associated with risk sexual behaviours, and provide evidence of leverage for effective interventions among male college students.
Methods
The cross-sectional study was conducted from December 2020 to December 2021 in 16 colleges, located in Sichuan Province, one of the high-risk areas in China. Overall 1640 male college students who reported sexually experienced were analyzed in this study. Multivariable logistic regression analysis was applied to determine factors associated with risk sexual behaviours.
Results
The average age of included male students was 19.95 ± 1.56. Of them, 27.74% initiated sexual behavior early, and 16.52% did not use condoms at the latest sexual intercourse. Students who were older (age 20-21, AOR=0.43, 95%CI: 0.33-0.55; age≥22, AOR=0.13, 95%CI: 0.08-0.21) (versus age ≤ 19) were less likely to have early sexual debut. The likelihood of engaging in condomless sex at the latest sexual intercourse was lower among male college students who had a senior high school educated mother (vs junior high school and below, AOR=0.47, 95%CI: 0.28-0.80), were knowledgeable about sex and reproductive health (vs unknowledgeable, AOR=0.48, 95%CI: 0.27-0.85), and held a higher level of condom use efficacy confident vs unconfident, AOR=0.50, 95%CI: 0.35-0.71; very confident vs unconfident, AOR=0.26, 95%CI: 0.16-0.42). The odds of condomless sex at the latest sexual intercourse increased among participants reporting perceived risk of HIV infection (vs no, AOR=1.96, 95%CI: 1.27-3.03) and early sexual debut (vs no, AOR=1.61, 95%CI: 1.12-2.32).
Conclusion
Comprehensive sex education curricula might be an effective approach by emphasizing fundamental sex and reproductive information and empowering students with assertiveness and negotiation skills with regard to condom use during or before college.
An optimized CT-dense agent perfusion and micro-CT imaging protocol for chick embryo developmental stages
Authors: Naija Azza , Mutlu Onur , Khan Talha , Seers Thomas Daniel , Yalcin Huseyin C
Published: February 2023

Compared to classical techniques of morphological analysis, micro-CT (µ-CT) has become an effective approach allowing rapid screening of morphological changes. In the present work, we aimed to provide an optimized µ-CT dense agent perfusion protocol and µ-CT guidelines for different stages of chick embryo cardiogenesis. Our study was conducted over a period of 10 embryonic days (Hamburger-Hamilton HH36) in chick embryo hearts. During the perfusion of the µ-CT dense agent at different developmental stages (HH19, HH24, HH27, HH29, HH31, HH34, HH35, and HH36), we demonstrated that durations and volumes of the injected contrast agent gradually increased with the heart developmental stages contrary to the flow rate that was unchanged during the whole experiment. Analysis of the CT imaging confirmed the efficiency of the optimized parameters of the heart perfusion.
Synonymous Codon Pattern of Cauliflower Mosaic Virus in phylogeny and evolution
Authors: Pouresmaeil Mahin , Pazhouhandeh Maghsoud , Shirzad Akbar , Khosroushahi Ahmad Yari
Published: February 2023

Cauliflower Mosaic Virus (CaMV) is a plant Pararetrovirus with a double-stranded DNA genome distributed worldwide. This study analyzed migration, evolution, and synonymous codon pattern of CaMV and the factors that shape it. We extracted genomic sequences of 121 isolates of CaMV, which were reported from various regions-hosts, from the NCBI database. The evolution of viruses has been widely studied by analyzing their nucleotides and coding regions/codons using different methods. Analysis of the CaMV phylogenetic tree shows that it divides most of the sequences into two main groups: Group I includes Irananin, Japanese, and American-European subgroups, and Group II includes Grecian, Turkish, and Iranian subgroups. Analysis of effective codon count, and relative codon deoptimization index, showed that natural selection is a major driving force in CaMV. Furthermore, Relative synonymous codon usage (RSCU) and neutrality analyses show that CaMV prefers A-ending codons and that one codon, namely GGA, was overrepresented. Analysis of dinucleotide composition demonstrates that nucleotide A was the most abundant in the CaMV coding sequences, and that the most frequent nucleotide at the third position of the codon was A3S. In CaMV, host adaptation was highest for Brassica oleracea and lowest for Raphanus sativus. Therefore the CaMV codon pattern is mostly shaped by the need to escape antiviral responses associated with host dinucleotides and translational efficiency. These values indicate that the study provides useful information on the codon usage analysis of CaMV and can be used to understand host adaptation to the virus environment and its evolution. This is the first study on codon usage bias of CaMV in the world.
Analysis of Prognostic Value, Immune Escape Significance and Ferroptosis Correlation of Ubiquitination-related Genes UBTD1 in Colorectal Carcinogenesis
Authors: Jin Yuzhao , Chen Qianping , Tang Bufu , Mao Wei , Wang Lu , Lan Huiyin , Bai Minghua , Zhu Ji , jiang jin
Published: February 2023

Immunotherapy based on immune checkpoint inhibitors is currently a hot topic of investigation in the therapy of colorectal cancer (CRC). The most reliable predictors of immune effectiveness are, at present, microsatellite instability (MSI) and mismatch repair gene status (MMR).In contrast, other immune efficacy predictors remain desirable to facilitate individualized immunotherapy for CRC patients. Ubiquitination and its associated ubiquitin-binding enzymes and ligases regulate the tumor microenvironment and antitumor immunity to mediate tumor pathogenesis and progression. Here, we examined the molecular characteristics and immunomodulatory effects of ubiquitination-associated genes mediating the prognosis of CRC cancer. UBTD1 was identified as a significant prognostic, predictive gene for CRC, involved in regulating the immune checkpoint levels and immune cell function of CRC patients. Briefly, high expression of UBTD1 tended to enhance the presence of immune checkpoints to induce immune escape and inhibit the onset of ferroptosis. Our study demonstrated that UBTD1 was a prognostic marker for CRC in the regulation of ubiquitination and the tumor immune microenvironment and may serve as a predictor of immune efficacy and a modulator of resistance to immune checkpoint suppressive therapies.