bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of older adults by a geriatric assessment–guided multidisciplinary clinic before CAR T-cell therapy
Authors: Yates Samuel J. , Cursio John F. , Artz Andrew , Kordas Keriann , Bishop Michael R. , Derman Benjamin A. , Kosuri Satyajit , Riedell Peter A. , Kline Justin , Jakubowiak Andrzej , Mortel Mylove , Johnson Shalitha , Nawas Mariam T.
Published: May 2024

The optimal means of assessing candidacy of older adults (≥65 years) for chimeric antigen receptor T-cell (CAR-T) therapy are unknown. We explored the role of a geriatric assessment (GA)–guided multidisciplinary clinic (GA-MDC) in selecting and optimizing older adults for CAR-T. Sixty-one patients were evaluated in a GA-MDC (median age, 73 years; range, 58-83). A nonbinding recommendation (“proceed” or “decline”) regarding suitability for CAR-T was provided for each patient based on GA results. Fifty-three patients ultimately received CAR-T (proceed, n = 47; decline, n = 6). Among patients who received B-cell maturation antigen (BCMA)–directed (n = 11) and CD19-directed CAR-T (n = 42), the median overall survival (OS) was 14.2 months and 16.6 months, respectively. GA uncovered high rates of geriatric impairment among patients proceeding to CAR-T therapy, with fewer impairments in those recommended “proceed.” Patients recommended “proceed” had shorter median length of stay (17 vs 31 days; P = .05) and lower rates of intensive care unit admission (6% vs 50%; P = .01) than those recommended “decline.” In patients receiving CD19- and BCMA–directed CAR-T therapy, a “proceed” recommendation was associated with superior OS compared with “decline” (median, 16.6 vs 11.4 months [P = .02]; and median, 16.4 vs 4.2 months [P = .03], respectively). When controlling for Karnofsky performance status, C-reactive protein, and lactate dehydrogenase at time of lymphodepletion, the GA-MDC treatment recommendation remained prognostic for OS (hazard ratio, 3.26; P = .04). Patients optimized via the GA-MDC without serious vulnerabilities achieved promising outcomes, whereas patients with high vulnerability experienced high toxicity and poor outcomes after CAR-T therapy.
PTCy vs CNI–based GVHD prophylaxis in HLA-matched transplants for Hodgkin lymphoma: a study of the LWP of the EBMT
Authors: Montoro Juan , Ngoya Maud , Kulagin Alexander , Giebel Sebastian , Broers Annoek E. C. , Bramanti Stefania , Halahleh Khalid , Pérez-Simón Jose A. , Solano Carlos , Ozcelik Tulay , Blaise Didier , Sanz Jaime , Henriques Marta , Peffault de Latour Régis , Martino Rodrigo , Scheid Christof , Fox Laura , Gromek Tomasz , Jurado Manuel , Sakellari Ioanna , Van Gorkom Gwendolyn , Matteucci Paola , Nagler Arnon , Koc Yener , Glass Bertram
Published: May 2024

Studies comparing the efficacy of posttransplant cyclophosphamide (PTCy) to conventional calcineurin inhibitor (CNI)–based graft-versus-host disease (GVHD) prophylaxis regimens in patients with Hodgkin lymphoma (HL) are scarce. This study aimed to compare the outcomes of patients with HL undergoing hematopoietic stem cell transplantation (HSCT) from HLA-matched donors who received GVHD prophylaxis with either PTCy- or conventional CNI-based regimens, using data reported in the European Society for Blood and Marrow Transplantation database between January 2015 and December 2022. Among the cohort, 270 recipients received conventional CNI-based prophylaxis and 176 received PTCy prophylaxis. Notably, PTCy prophylaxis was associated with delayed hematopoietic recovery but also with a lower risk of chronic (25% vs 43%; P < .001) and extensive chronic GVHD (13% vs 28%; P = .003) compared with the CNI-based cohort. The 2-year cumulative incidence of nonrelapse mortality and relapse was 11% vs 17% (P = .12) and 17% vs 30% (P = .007) for PTCy- and CNI-based, respectively. Moreover, the 2-year overall survival (OS), progression-free survival (PFS), and GVHD-free, relapse-free survival (GRFS) were all significantly better in the PTCy group compared with the CNI-based group: 85% vs 72% (P = .005), 72% vs 53% (P < .001), and 59% vs 31% (P < .001), respectively. In multivariable analysis, PTCy was associated with a lower risk of chronic and extensive chronic GVHD, reduced relapse, and better OS, PFS, and GRFS than the CNI-based platform. Our findings suggest that PTCy as GVHD prophylaxis offers more favorable outcomes than conventional CNI-based prophylaxis in adult patients with HL undergoing HSCT from HLA-matched donors.
Airway IL-1β is related to disease severity and mucociliary function in bronchiectasis
Authors: Perea Lidia , Bottier Mathieu , Cant Erin , Richardson Hollian , Dicker Alison J. , Shuttleworth Morven , Giam Yan Hui , Abo-Leyah Hani , Finch Simon , Huang Jeffrey T-J. , Shteinberg Michal , Goeminne Pieter C. , Polverino Eva , Altenburg Josje , Blasi Francesco , Welte Tobias , Aliberti Stefano , Sibila Oriol , Chalmers James D. , Shoemark Amelia
Published: May 2024

Rationale

The inflammasome is a key regulatory complex of the inflammatory response leading to interleukin-1β (IL-1β) release and activation. IL-1β amplifies inflammatory responses and induces mucus secretion and hyperconcentration in other diseases. The role of IL-1β in bronchiectasis has not been investigated.

Objectives

To characterise the role of airway IL-1β in bronchiectasis, including the association with mucus properties, ciliary function, airway inflammation, microbiome and disease severity.

Methods

Stable bronchiectasis patients were enrolled in an international cohort study (n=269). IL-1β was measured in sputum supernatant. A validation cohort also had sputum rheology and hydration measured (n=53). For analysis, patients were stratified according to the median value of IL-1β in the population (highversuslow) to compare disease severity, airway infection, microbiome (16S rRNA sequencing), inflammation and caspase-1 activity. Primary human nasal epithelial cells grown in air–liquid interface culture were used to study the effect of IL-1β on cilia function.

Results

Patients with high sputum IL-1β had more severe disease, increased caspase-1 activity and an increased T-helper type 1, T-helper type 2 and neutrophil inflammatory response compared with patients with low IL-1β. The active-dominant form of IL-1β was associated with increased disease severity. High IL-1β was related to higher relative abundance of Proteobacteria in the microbiome and increased mucus solid content and viscoelastic properties. Chronic IL-1β treatment reduced the functionality of cilia and tight junctions of epithelial cellsin vitro.

Conclusions

A subset of stable bronchiectasis patients show increased airway IL-1β, suggesting pulmonary inflammasome activation is linked with more severe disease, airway infection, mucus dehydration and epithelial dysfunction.
Factors predicting resolution of left ventricular thrombus in different time windows after myocardial infarction
Authors: Lu Zhen , Song Bingxue , Liu Xin , Zhang Ning , Li Fuhai , Xu Fengqiang , Lian Zhexun , Guo Junjie
Published: May 2024

Background

Left ventricular thrombus (LVT) is a serious complication after myocardial infarction. However, due to its asymptomatic nature, early detection is challenging. We aimed to explore the differences in clinical correlates of LVT found in acute to subacute and chronic phases of myocardial infarction.

Methods

We collected data from 153 patients who were diagnosed with LVT after myocardial infarction at the Affiliated Hospital of Qingdao University from January 2013 to December 2022. Baseline information, inflammatory markers, transthoracic echocardiograph (TTE) data and other clinical correlates were collected. Patients were categorized into acute to subacute phase group (< 30 days) and chronic phase group (30 days and after) according to the time at which echocardiograph was performed. The resolution of thrombus within 90 days is regarded as the primary endpoint event. We fitted logistic regression models to relating clinical correlates with phase-specific thrombus resolution.

Results

For acute to subacute phase thrombus patients: C-reactive protein levels (OR: 0.95, 95% CI: 0.918–0.983, p = 0.003) were significantly associated with thrombus resolution. For chronic phase thrombus patients: anticoagulant treatment was associated with 5.717-fold odds of thrombus resolution (OR: 5.717, 95% CI: 1.543–21.18, p = 0.009).

Conclusions

Higher levels of CRP were associated with lower likelihood of LVT resolution in acute phase myocardial infarction; Anticoagulant therapy is still needed for thrombus in the chronic stage of myocardial infarction.
HF Etiology and cardiac contractility modulation therapy
Authors: Davtyan Karapet , Chugunov Ivan , Topchyan Arpi , Mareev Yury , Mironova Natalia , Rimskaya Elena , Golitsyn Sergey , Mikhaylov Evgeny , Lebedev Dmitry , Vander Marianna , Lyasnikova Elena , Sitnikova Maria , Minjia Khatuna , Glembo Svetlana , Sukhorukov Oleg
Published: May 2024

Objectives

Our study aimed to assess the safety and efficacy of cardiac contractility modulation (CCM) therapy in patients with heart failure with reduced ejection fraction (HFrEF) depending on HF etiology.

Methods

We enrolled 166 patients with optimal medical therapy-resistant HFrEF (median age 59 years, 83.7% males, median NYHA class − 2, median left ventricular ejection fraction (LVEF) − 29.0%) who underwent CCM therapy device implantation from 2013 to 2019 in four medical centers in Russia. The HF etiology was determined based on invasive coronary angiography or cardiac MRI data. Transthoracic echocardiography (TTE), 6-minute walking test (6MWT), and NTproBNP-tests were performed at a baseline and 12 months after the implantation.

Results

The ischemic etiology of HF was revealed in 100 patients (61.5%) (ICM group); the non-ischemic group (NICM) evolved 66 patients (38.5%). Patients in the ICM group were significantly older (61[57–69] vs. 55 [42.8–61], p < 0.001), more frequently had hypertension (79% vs. 42.4%, p < 0.001) and chronic kidney disease (43% vs. 22.7%, p = 0.012). Patients in the NICM group had significantly more often atrial fibrillation (AF) (58% vs. 74%, p = 0.048), larger end-diastolic volume (EDV) (249 [208–309] vs. 220 [192–271], p = 0.019) and end-systolic volume (ESV) (183 [147–230] vs. 154 [128–199], p = 0.003). There were no significant differences in mortality between ICM and NICM groups (14.4 vs. 10.8%, p = 0.51). In 12 months, there was a significant increase in LVEF in the NICM group (+ 2.0 [2–6] vs. +7.7 [2–12], p < 0.001), while the improvement in the 6MWT (+ 75 [22–108] vs. +80 [10–160], p = 0.851) and NYHA class did not reach the level of significance. The subanalysis between patients with improved NYHA class and those without improvement revealed that patients without improvement more frequently had AF (56% vs. 89%; p < 0.01), chronic obstructive lung disease (18% vs. 35% p = 0.047), higher blood pressure (110 [105–120] vs. 120[110–129]; p = 0.032).

Conclusion

In this multicenter retrospective study, patients with non-ischemic HFrEF showed a significantly higher improvement in LVEF and LV reverse remodeling following CCM therapy device implantation. There was no significant association between HF etiology and survival in drug-resistant HFrEF patients following CCM therapy.
Patient safety incidents in Irish general practice during the COVID-19 pandemic: an exploratory practice level analysis
Authors: Garzón-Orjuela Nathaly , Collins Claire , Willems Sara , Van Poel Esther , Vellinga Akke
Published: May 2024

Background

Patient safety is defined as the prevention of harm to patients and aims to prevent errors. This analysis explores factors associated with the reported occurrence of patient safety incidents (PSIs) in general practices in Ireland at the start of the COVID-19 pandemic.

Methods

The PRICOV-19 was a cross-sectional study to record the (re)organisation of care provided in general practice and changes implemented during the COVID-19 pandemic in 38 countries. Primary outcomes include three potential scenarios of PSIs: delayed care due to practice factors, delayed care due to patient factors, and delayed care due to triage. Exploratory variables included demographic and organisational characteristics, triage, collaboration, and strategies to safeguard staff members’ well-being.

Results

Of the 172 participating Irish general practices, 71% (n = 122) recorded at least one potential PSI. The most frequent incident was delayed care due to patient factors (65%), followed by practice (33%) and triage (30%). Multivariate analysis showed that delayed care due to patient factors was associated with changes in the process of repeat prescriptions (OR 6.7 [CI 95% 2.5 to 19.6]). Delayed care due to practice factors was associated with suburbs/small towns (OR 4.2 [1.1 to 19.8]) and structural changes to the reception (OR 3.5 [1.2 to 11.4]). While delayed care due to patient factors was associated with having a practice population of 6000–7999 patients (OR 4.7 [1.1 to 27.6]) and delayed care due to practice factors was associated with having a practice population of 2000–3999 patients (OR 4.2 [1.2 to 17.1]). No linear associations were observed with higher or lower patient numbers for any factor. Delayed care due to triage was not associated with any exploratory variables.

Conclusion

The COVID-19 pandemic resulted in dramatic changes in the delivery of care through general practices in Ireland. Few factors were associated with the reported occurrence of PSIs, and these did not show consistent patterns. Sustained improvements were made in relation to repeat prescriptions. The lack of consistent patterns, potentially confirms that the autonomous decisions made in general practice in response to the challenges of the COVID-19 pandemic could have benefitted patient safety (See Graphical abstract).

Graphical Abstract
High frequency of alpha7-HPV in Colombian Caribbean coast women: cervical cancer screening analysis
Authors: Arteaga-Pautt Heiser , Bru-Cordero O. Elias , Ricardo-Caldera Dina , Espitia-Pérez Lyda , Avilés-Vergara Paula , Tovar-Acero Catalina , Castaño-Caraballo Lorena , Perdomo-Lara Sandra Janeth , Zetién-Arteaga Helvey Ramón , Behaine-Bravo Valentina , Soto-De León Sara Cecilia
Published: May 2024

Background

Cervical cancer (CC) is a significant global public health concern, particularly in developing countries such as Colombia. The main risk factor involves high-risk HPV types (HR-HPV) infection, coupled with population-specific variables. The Caribbean region in Colombia lacks research on HR-HPV-type frequencies. Therefore, this study aims to establish the prevalence of type-specific HR-HPV and its association with sociodemographic factors among women undergoing cervical cytology screening.

Methods

A cross-sectional study involving voluntary women who provided informed consent and completed a questionnaire capturing sociodemographic, clinical, and sexual behavior information was conducted. All participants underwent cervical cytology and molecular analysis. Generic HPV detection employed three simultaneous PCRs (GP5+/6+, MY09/11, and PU1R/2 M), and positive samples were genotyped using the Optiplex HPV Genotyping kit. The analysis encompassed the 12 types of high-risk HPV (HR-HPV-16,-18,-31,-33,-35,-39,-45,-51,-52,-56,-58, and − 59). Frequencies were reported based on geographic subregions within the Córdoba department, and disparities were made between single and multiple infections. Sociodemographic and clinical variables were subjected to ordinal logistic regression, with statistical significance at a p-value < 0.05. The statistical analyses utilized STATA 14® and R-Core Team-software.

Results

We included 450 women, mean age 40 (SD$$\pm$$11.44). PCR analysis revealed 43% HPV-positive (n=192). GP5+/6+ detected the most positives at 26% (n=119), followed by PU1R/2 M at 22% (n = 100) and MY09/11 at 15% (n=69). Multiple infections occurred in 87.3% (n=142), primarily 2 to 4 types (47.37%, n=90). Dominant types were HPV-18 (15.6%, n=61), HPV-16 (14.9%, n=58), HPV-31 (13.0%, n = 51), and HPV-45 (11.5%, n=45). Logistic regression identified age above 60 as a risk for concurrent multiple types (OR=6.10; 95% CI 1.18–31.63). Menopause was protective (OR=0.31; 95% CI 0.11–0.89).

Conclusions

Our study reveals a notable prevalence of multiple (2–4) high-risk HPV infections among adult women engaged in CC detection initiatives. Predominantly, α7 species constitute the prevalent HR-viral types, with the Medio Sinú subregion showing elevated prevalence. Menopausal status confers protection against diverse HR-HPV infections. Nevertheless, advancing age, particularly beyond 60 years, is linked to an increased susceptibility to simultaneous infections by multiple HPV-types.
Survival and disease burden analyses of occupational pneumoconiosis during 1958–2021 in Huangshi city, China: a retrospective cohort study
Authors: Chen Hai-Lian , Li Chun-Hu , Zhai Pei-Yao , Zhuang Xun , Lian Yu-Long , Qiao Xue , Feng Jian , Qian Zu-Shu , Qin Gang
Published: May 2024

Background

Pneumoconiosis, a chronic disease stemming from prolonged inhalation of dust particles, stands as a significant global burden of occupational diseases. This study aims to investigate the survival outcomes of pneumoconiosis patients in Huangshi city, China, while also evaluating the disease burden on afflicted patients.

Methods

Data for this study were sourced from the Huangshi Center for Disease Control and Prevention. Survival analyses of pneumoconiosis patients were conducted employing life tables and the Kaplan-Meier method. The Cox proportional hazards models were deployed to identify factors influencing pneumoconiosis patients’ survival duration. Competing risks models were employed to confirm the validity of the model outcomes. Additionally, in the disease burden assessment, disability-adjusted life years (DALYs) were computed for various demographic groups and time frames.

Results

A total of 5,641 pneumoconiosis cases, diagnosed in Huangshi City, Hubei Province between 1958 and 2021, were incorporated into the cohort analysis. The probability of mortality and the risk ratio increased with advancing age. Notably, the median survival time of stage III pneumoconiosis patients was significantly shorter compared with those in stages I and II. The Cox proportional hazards model and competing risks analyses underscored several significant factors influencing survival time, including dust exposure duration (HR = 1.197, 95% CI: 1.104–1.298), age at first diagnosis (HR = 3.149, 95% CI: 2.961–3.349), presence of silicosis (HR = 1.378, 95% CI: 1.254–1.515), and stage II-III pneumoconiosis (HR = 1.456, 95% CI: 1.148–1.848). Cumulatively, DALYs amounted to 7,974.35 person-years, with an average of 1.41 person-years. The period between 2000 and 2019 witnessed the highest disease burden.

Conclusion

Our findings highlight the urgent need for improved prevention, earlier detection, and more effective management strategies for the occupational pneumoconiosis population. This study not only underscores the persistent issue of pneumoconiosis in industrial environments but also serves as a crucial call to action for policymakers and healthcare providers.
Prioritization of ethical concerns regarding HIV molecular epidemiology by public health practitioners and researchers
Authors: Schuster Anne L. R. , Bollinger Juli , Geller Gail , Little Susan J. , Mehta Sanjay R. , Sanchez Travis , Sugarman Jeremy , Bridges John F. P.
Published: May 2024

Background

HIV molecular epidemiology (HIV ME) can support the early detection of emerging clusters of new HIV infections by combining HIV sequence data routinely obtained during the clinical treatment of people living with HIV with behavioral, geographic, and sociodemographic information. While information about emerging clusters promises to facilitate HIV prevention and treatment efforts, the use of this data also raises several ethical concerns. We sought to assess how those working on the frontlines of HIV ME, specifically public health practitioners (PHPs) and researchers, prioritized these issues.

Methods

Ethical issues were identified through literature review, qualitative in-depth interviews, and stakeholder engagement. PHPs and researchers using HIV ME prioritized the issues using best–worst scaling (BWS). A balanced incomplete block design was used to generate 11 choice tasks each consisting of a sub-set of 5 ethical concerns. In each task, respondents were asked to assess the most and least concerning issue. Data were analyzed using conditional logit, with a Swait-Louviere test of poolability. Latent class analysis was then used to explore preference heterogeneity.

Results

In total, 57 respondents completed the BWS experiment May–June 2023 with the Swait-Louviere test indicating that researchers and PHPs could be pooled (p = 0.512). Latent class analysis identified two classes, those highlighting “Harms” (n = 29) (prioritizing concerns about potential risk of legal prosecution, individual harm, and group stigma) and those highlighting “Utility” (n = 28) (prioritizing concerns about limited evidence, resource allocation, non-disclosure of data use for HIV ME, and the potential to infer the directionality of HIV transmission). There were no differences in the characteristics of members across classes.

Conclusions

The ethical issues of HIV ME vary in importance among stakeholders, reflecting different perspectives on the potential impact and usefulness of the data. Knowing these differences exist can directly inform the focus of future deliberations about the policies and practices of HIV ME in the United States.
The interaction of occupational stress and job burnout on depressive symptoms in railway workers in Fuzhou city
Authors: Yu Shuangjie , Zhao Min , Li Ye , Liu Can , Fu Youjuan , Jiang Yu , Guan Suzhen
Published: May 2024

Background

To explore the relationship between occupational stress, burnout and depressive symptoms among railroad workers in Fuzhou, and to analyze the interaction of burnout and occupational stress on depressive symptoms.

Methods

In this study, 861 railway employees of Fuzhou railway bureau were randomly selected from January to April, 2022. Occupational stress inventory revised edition (OSI-R), China job burnout inventory (CMBI) and Symptom Checklist-90 (SCL-90) were used to investigate the occupational stress, job burnout and depressive symptoms of railway workers. Interactions associated with depressive symptoms were assessed by linear hierarchical regression analysis and SPSS macros (PROCESS).

Results

Occupational stress, job burnout and depressive symptoms accounted for 50.58%, 93.47%, and 11.19% of the study population, respectively. There were intergroup differences between age, marriage status, and length of service (P < 0.05). Occupational stress and job burnout are the main risk factors for depressive symptoms (OR: 2.01, 95%CI: 1.17–3.45; 1.94, 1.69–2.23, respectively). More importantly, further analysis of the interaction between occupational stress and job burnout showed that those with high levels of job burnout had a high-risk effect on depressive symptoms at high levels of occupational stress.

Conclusion

Occupational stress and job burnout are risk factors for depressive symptoms among railroad workers in Fuzhou City. The interaction of job burnout and occupational stress increases the risk of depressive symptoms.
Validation of an Arabic version of the short form of the health literacy in dentistry scale: a cross-sectional study
Authors: Alzeer Muneera Essa , AlJameel AlBandary Hassan , Rosing Kasper , Øzhayat Esben Boeskov
Published: May 2024

Background

The Arabic version of the short form of the Health Literacy in Dentistry scale (HeLD) had not yet been developed in previously published studies. This study aims to test the reliability and validity of an Arabic version of the short form of the HeLD questionnaire.

Methods

The short version of HeLD was translated into Arabic and the psychometric properties were evaluated in a sample of 1,889 female students in their first year of secondary school. Test–retest reliability was assessed using the intra-class correlation coefficient (ICC), and internal consistency reliability was assessed using Cronbach’s alpha. Content validity was investigated by creating a correlation matrix between the individual items of the HeLD-14, and criterion validity was determined using Pearson’s correlation between the HeLD-14 score and an overall oral health rating. Sensitivity analysis was assessed by testing the associations of the HeLD-14 score with oral health-related behaviours and residential area.

Results

The Arabic version of HeLD-14 (A-HeLD-14) had acceptable ICC (0.54) and excellent internal consistency (Cronbach’s alpha: 0.92). The correlations between the items of the A-HeLD-14 varied from 0.3 to 0.9. The A-HeLD-14 showed a statistically significant correlation with the overall oral health rating (r = 0.37, p < 0.001). The median A-HeLD-14 score was significantly higher in participants who brushed their teeth frequently (51.31), visited the dentist regularly (52.00), consumed fresh fruit frequently (51) and consumed soda or energy drinks infrequently (51.00) than participants who brushed their teeth infrequently (41.50), visited the dentist irregularly (49.00), consumed fresh fruit infrequently (47) and consumed soda or energy drinks frequently (48.00).

Conclusion

The A-HeLD-14 instrument demonstrates sufficient validity, reliability, and sensitivity for measuring oral health literacy among the Arabic-speaking population.
Antimicrobial activity of cleanser tablets against S. mutans and C. albicans on different denture base materials
Authors: Geduk Şükriye Ece , Sağlam Gaye , Cömert Füsun , Geduk Gediz
Published: May 2024

Background

In this study, the antimicrobial activity of three different cleanser tablets on S. mutans and C. albicans adhesion to PMMA, polyamide and 3D printed resin was investigated.

Methods

40 samples were prepared for PMMA (SR Triplex Hot), polyamide (Deflex) and 3D printed resin (PowerResins Denture) materials and divided into four subgroups for cleansers (Aktident™, Protefix™, Corega™ tablets and distilled water) (n = 5). After the surface preparations were completed, the samples were immersed separately in tubes containing the prepared microorganism suspension and incubated at 37˚C for 24 h. After the incubation, the samples were kept in the cleanser solutions. The samples were then transferred to sterile saline tubes. All the tubes were vortexed and 10 µl was taken from each of them. Sheep blood agar was inoculated for colony counting. The inoculated plates were incubated for 48 h for S. mutans and 24 h for C. albicans. After incubation, colonies observed on all plates were counted. Statistical analyses were done with three-way ANOVA and Tukey’s multiple comparison test.

Results

Polyamide material registered the highest colony count of S. mutans, whereas PMMA registered the lowest. Significant differences in S. mutans adherence (p = 0.002) were found between the three denture base materials, but no such difference in C. albicans adherence (p = 0.221) was identified between the specimens. All three cleanser tablets eliminated 98% of S. mutans from all the material groups. In all these groups, as well, the antifungal effect of Corega™ on C. albicans was significantly higher than those of the other two cleanser tablets.

Conclusions

According to the study’s results, it may be better to pay attention to surface smoothness when using polyamide material to prevent microorganism retention. Cleanser tablets are clinically recommended to help maintain hygiene in removable denture users, especially Corega tablets that are more effective on C. albicans.
Comparison of triglyceride glucose index and modified triglyceride glucose indices in prediction of cardiovascular diseases in middle aged and older Chinese adults
Authors: Cui Cancan , Qi Yitian , Song Jiayin , Shang Xinyun , Han Tianjiao , Han Ning , Yue Siqi , Zha Yining , Xu Zhonghang , Li Jiannan , Liu Lin
Published: May 2024

Background

Triglyceride and glucose (TyG) index, a surrogate marker of insulin resistance, has been validated as a predictor of cardiovascular disease. However, effects of TyG-related indices combined with obesity markers on cardiovascular diseases remained unknown. We aimed to investigate the associations between TyG index and modified TyG indices with new-onset cardiovascular disease and the time-dependent predictive capacity using a national representative cohort.

Methods

This study is a retrospective observational cohort study using data from China Health and Retirement Longitudinal Study (CHARLS) of 7 115 participants. The TyG index was calculated as Ln [fasting triglyceride (mg/dL) × fasting glucose (mg/dL)/2]. The modified TyG indices were developed combining TyG with body mass index (BMI), waist circumference (WC) and waist-to‐height ratio (WHtR). We used adjusted Cox proportional hazards regression to analyze the association and predictive capacity based on hazard ratio (HR) and Harrell’s C‐index.

Results

Over a 7-year follow‐up period, 2136 participants developed cardiovascular disease, including 1633 cases of coronary heart disease and 719 cases of stroke. Compared with the lowest tertile group, the adjusted HR (95% CI) for new-onset cardiovascular disease in the highest tertile for TyG, TyG-BMI, TyG-WC, and TyG-WHtR were 1.215 (1.088–1.356), 1.073 (0.967–1.191), 1.078 (0.970–1.198), and 1.112 (1.002–1.235), respectively. The C‐indices of TyG index for cardiovascular disease onset were higher than other modified TyG indices. Similar results were observed for coronary heart disease and stroke.

Conclusion

TyG and TyG-WhtR were significantly associated with new-onset cardiovascular diseases, and TyG outperformed the modified TyG indices to identify individuals at risk of incident cardiovascular event.
A combination of a TLR7/8 agonist and an epigenetic inhibitor suppresses triple-negative breast cancer through triggering anti-tumor immune
Authors: Jiang Zhenzhen , Cai Guangqing , Liu Haiting , Liu Leping , Huang Rong , Nie Xinmin , Gui Rong , Li Jian , Ma Jinqi , Cao Ke , Luo Yanwei
Published: May 2024

Background

Combination therapy involving immune checkpoint blockade (ICB) and other drugs is a potential strategy for converting immune-cold tumors into immune-hot tumors to benefit from immunotherapy. To achieve drug synergy, we developed a homologous cancer cell membrane vesicle (CM)-coated metal-organic framework (MOF) nanodelivery platform for the codelivery of a TLR7/8 agonist with an epigenetic inhibitor.

Methods

A novel biomimetic codelivery system (MCM@UN) was constructed by MOF nanoparticles UiO-66 loading with a bromodomain-containing protein 4 (BRD4) inhibitor and then coated with the membrane vesicles of homologous cancer cells that embedding the 18 C lipid tail of 3M-052 (M). The antitumor immune ability and tumor suppressive effect of MCM@UN were evaluated in a mouse model of triple-negative breast cancer (TNBC) and in vitro. The tumor immune microenvironment was analyzed by multicolor immunofluorescence staining.

Results

In vitro and in vivo data showed that MCM@UN specifically targeted to TNBC cells and was superior to the free drug in terms of tumor growth inhibition and antitumor immune activity. In terms of mechanism, MCM@UN blocked BRD4 and PD-L1 to prompt dying tumor cells to disintegrate and expose tumor antigens. The disintegrated tumor cells released damage-associated molecular patterns (DAMPs), recruited dendritic cells (DCs) to efficiently activate CD8+ T cells to mediate effective and long-lasting antitumor immunity. In addition, TLR7/8 agonist on MCM@UN enhanced lymphocytes infiltration and immunogenic cell death and decreased regulatory T-cells (Tregs). On clinical specimens, we found that mature DCs infiltrating tumor tissues of TNBC patients were negatively correlated with the expression of BRD4, which was consistent with the result in animal model.

Conclusion

MCM@UN specifically targeted to TNBC cells and remodeled tumor immune microenvironment to inhibit malignant behaviors of TNBC.
Uncommon presentations of type A quadricuspid aortic valve in the Septuagenarian
Authors: Choi Perry , Paulsen Michael , Lin Yihan , Moskalik William , Ji Angela , Jackson Ethan , Malik Sachin , Burton Elan , Woo Y Joseph , Burdon Thomas
Published: May 2024

Background

Quadricuspid aortic valve (QAV) is a rare congenital anomaly characterized by the presence of four cusps instead of the usual three. It is estimated to occur in less than 0.05% of the population, with Type A (four equal-sized leaflets) accounting for roughly 30% of QAV subtypes. Based on limited clinical series, the usual presentation is progressive aortic valve regurgitation (AR) with symptoms occurring in the fourth to sixth decade of life. Severe aortic valve stenosis (AS) and acute AR are very uncommon.

Case presentation

We describe two cases of Type A QAV in patients who remained asymptomatic until their seventies with very uncommon presentations: one with severe AS and one with acute, severe AR and flail leaflet. In Case A, a 72-year-old patient with history of moderate AS presents to clinic with progressive exertional dyspnea. During work-up for transcatheter vs. surgical replacement pre-operative computed tomography angiogram (CTA) reveals a quadricuspid aortic valve with severe AS, and the patient undergoes surgical aortic valve replacement. Pre-discharge transthoracic echocardiography (TTE) shows good prosthetic valve function with no gradient or regurgitation. In Case B, a 76-year-old patient is intubated upon arrival to the hospital for acute desaturation, found to have wide open AR on catheterization, and transferred for emergent intervention. Intraoperative TEE reveals QAV with flail leaflet and severe AR. Repair is considered but deferred ultimately due to emergent nature. Post-operative TTE demonstrates good prosthetic valve function with no regurgitation and normal biventricular function.

Conclusions

QAV can present as progressive severe AS and acute AR, with symptoms first occurring in the seventh decade of life. The optimal treatment for QAV remains uncertain. Although aortic valve repair or transcatheter option may be feasible in some patients, aortic valve replacement remains a tenable option.
Condensation methodology for quantification of Polymyxin B fluorimetrically: application to pharmaceutical formulations and greenness assessment
Authors: Abdelmajed Mahmoud A. , El-Din Khalid M. Badr , Attia Tamer Z. , Oraby Mohamed , Omar Mahmoud A.
Published: May 2024

The appearance of multidrug-resistant Gram-negative bacterial infections, along with the lack of newly discovered antibiotics, resulted in the return to old antimicrobial medications like Polymyxins. As a result, the suggested technique aims to develop a fast, environmentally friendly, and sensitive fluorimetric method for quantifying Polymyxin B. The investigated approach depends on generating a highly fluorescent derivative by a condensation pathway between the studied drug and ninhydrin in the presence of phenylacetaldehyde and then estimated spectrofluorimetrically. After the reaction conditions were well optimized, the fluorescent product was estimated at emission wavelength (λem) = 475.5 nm (following excitation at a wavelength (λex) = 386 nm. The developed calibration plot displayed rectilinear throughout the following range (0.2-3 µg mL− 1), and the calculated limit of detection and quantification were 0.062 µg mL− 1 and 0.187 µg mL− 1, respectively. As a consequence, the drug’s ophthalmic and intravenous pharmaceutical forms were both successfully quantified with an excellent degree of recovery. Finally, the methodology’s greenness was assessed utilizing Analytical Eco-Scale scores.
Growth status and age at peak height velocity among youth participants in several sports: the Cracow longitudinal study
Authors: Kozieł Sławomir M. , Suder Agnieszka , Chrzanowska Maria , Králík Miroslav , Malina Robert M.
Published: May 2024

Background

Studies addressing age at peak height velocity (PHV) in longitudinal samples of participants in sports are relatively limited.

Purpose

To compare the growth status and estimated ages at PHV of longitudinal samples girls and boys active in sport with peers not active in sport, and to compare estimated ages at PHV among longitudinal samples of Polish youth active in sport.

Methods

Records from the Cracow Longitudinal Study, which measured youth annually from 8 to 18 years, were screened to identify individuals regularly active in sport. Participants in athletics (22 girls, 10 boys), soccer (12 boys), and other team (6 girls, 7 boys) and individual (6 girls, 9 boys) sports were identified; 107 girls and 172 boys were not active in sport. Heights and weights of participants in sports and non-involved peers were compared. Longitudinal height records of individuals were fit with the SITAR model to estimate age at PHV. Ages at PHV of boys and girls active in sport and peers not active in sports were compared with sex-specific ANOVAs.

Results

Ages at PHV of boys participating in athletics and soccer were similar to age at PHV of boys not active in sport, while ages at PHV of boys in other team sports (basketball, volleyball, handball) and individual sports (skiing, gymnastics, acrobatics) were, respectively, slightly earlier and later. Among girls, age at PHV of participants in team sports (basketball, netball) was earlier, while ages at PHV among participants in athletics and other individual sports (equestrian, acrobatics, shooting) were slightly later compared to non-athlete peers.

Conclusion

Ages at PHV varied among participants in different sports and were consistent with estimates in other longitudinal samples of Polish youth athletes.
Spreading code optimization for low-earth orbit satellites via mixed-integer convex programming
Authors: Yang Alan , Mina Tara , Gao Grace
Published: May 2024

Optimizing the correlation properties of spreading codes is critical for minimizing inter-channel interference in satellite navigation systems. By improving the codes’ correlation sidelobes, we can enhance navigation performance while minimizing the required spreading code lengths. In the case of low-earth orbit (LEO) satellite navigation, shorter code lengths (on the order of a hundred) are preferred due to their ability to achieve fast signal acquisition. Additionally, the relatively high signal-to-noise ratio in LEO systems reduces the need for longer spreading codes to mitigate inter-channel interference. In this work, we propose a two-stage block coordinate descent (BCD) method which optimizes the codes’ correlation properties while enforcing the autocorrelation sidelobe zero property. In each iteration of the BCD method, we solve a mixed-integer convex program over a block of 25 binary variables. Our method is applicable to spreading code families of arbitrary sizes and lengths, and we demonstrate its effectiveness for a problem with 66 length-127 codes and a problem with 130 length-257 codes.
Social farming and educational needs: how kindergarten farms could fill a gap
Authors: Paffarini Chiara , Torquati Biancamaria , Sannipoli Moira , Fabbri Alessia , Cecchini Lucio
Published: May 2024

Kindergarten farms are educational initiatives aiming to reinforce or even create relationships between nature and future generations involving them in agricultural settings. They take the form of Outdoor Education (OE), intending to enhance the outdoor environment in its various configurations, from an educational perspective. From the agricultural side, these activities are included in social farming (SF), represents a union between the educative sphere and the agriculture world and offering added farm revenue with a multifunctional view. In Italy, these educational services within the farms (‘Agrinidi’ and ‘Agriasili’) have grown recently, contributing to the educational system where services are marginally available or lacking. This study has focused on the families' needs concerning early childhood services in support of work–life balance; it also investigated their attitudes and interest in OE and in a structured educational service within a farm. To achieve such an aim, a literature study about OE and the educational initiatives within the farm in Italy was carried out. This was followed by  an online questionnaire, carried out with 510 resident of a region of Italy (Umbria). The findings indicate high interest in educational services organized in both nature and farms. The statistical and econometric analysis of a subsample of 161 respondents having children under 6 years old has underlined that the attitudes towards OE are affected by specific socio-demographic variables: income and educational levels. In this respect, older participants having a high degree of education and benefiting from a high economic status showed the highest level of preference for OE services. In the conclusions, we underline that developing educational services "Agriasili" and "Agrinidi" can contribute to strengthening the synergy between agriculture and the education sector. They carry out social functions for the creation of collective well-being. They represent a diversification of farm business and a response to citizens' needs for early childhood education in nature; moreover, they push implementation of policies encouraging the birth of these services in rural and peri-urban areas where they are lacking or crowded.
Hyperspectral lidar for monitoring high-resolution activity patterns of African stingless bee species
Authors: Månefjord Hampus , Huzortey A. Andrew , Boateng Rabbi , Gbogbo Y. Adolphe , Yamoa A. S. Doria , Zoueu Jérémie T. , Kwapong Peter K. , Anderson Benjamin , Brydegaard Mikkel
Published: May 2024

Background

Stingless bees are vital pollinators and honey producers in the tropics. Research on stingless bees is generally underrepresented compared to the western honeybees, and while stingless bee studies from some regions are reported, there is a particular lack of reports on the species endemic to Sub-Saharan Africa. Since conventional entomological methods such as mark-recapture and radar harmonic tags suffer from limited observation counts and amount to a significant payload, fluorescent powder tagging offers a promising alternative to understanding their behavior. We deploy a hyperspectral fluorescence lidar monitors a 25-mm-wide transect in front of the hives.

Results

During a 1 day study at the International Stingless Bee Center, near Kakum National Park, Ghana, 17,862 insects were observed with the lidar, of which 7520 were tagged with fluorescent dyes. Approximately half of the bees from the selected hives were successfully tagged, with an estimated misclassification of 1%. According to our limited data, the observed species, Meliponula bocandei and the Dactylurina staudingeri exhibited different activity patterns. D. staudingeri displayed a half-hour longer active day, with clear crepuscular activity peaks. In contrast, M. bocandei activity was diurnal, with less pronounced crepuscular peaks.

Conclusions

We demonstrate how hyperspectral fluorescence lidar can monitor powder-tagged insects throughout the day. The monitored species revealed distinct activity patterns over the day. Our findings highlight the potential of this technology as a valuable tool for understanding insect behavior and environmental preferences of species, in situ, which could potentially give clues of response to climate changes of these critical species.
Incarceration history and opioid use among adults living with HIV and chronic pain: a secondary analysis of a prospective cohort study
Authors: Lichtiger Anna B. , Deng Yuting , Zhang Chenshu , Groeger Justina , Perez Hector R. , Nangia Gayatri , Prinz Melanie , Richard Emma , Glenn Matthew , De La Cruz Ana Alicia , Pazmino Ariana , Cunningham Chinazo O. , Amico K Rivet , Fox Aaron , Starrels Joanna L.
Published: May 2024

Background

Adults living with HIV have disproportionately high chronic pain, prescription opioid use, history of substance use, and incarceration. While incarceration can have long-lasting health impacts, prior studies have not examined whether distant (>1 year prior) incarceration is associated with opioid use for chronic pain, or with opioid misuse or opioid use disorder among people living with HIV and chronic pain.

Methods

We conducted a secondary analysis of a prospective cohort study of adults living with HIV and chronic pain. The independent variables were any distant incarceration and drug-related distant incarceration (both dichotomous). Dependent variables were current long-term opioid therapy, current opioid misuse, and current opioid use disorder. A series of multivariate logistic regression models were conducted, adjusting for covariates.

Results

In a cohort of 148 participants, neither distant incarceration nor drug-related incarceration history were associated with current long-term opioid therapy. Distant incarceration was associated with current opioid misuse (AOR 3.28; 95% CI: 1.41-7.61) and current opioid use disorder (AOR 4.40; 95% CI: 1.54-12.56). Drug-related incarceration history was also associated with current opioid misuse (AOR 4.31; 95% CI: 1.53-12.17) and current opioid use disorder (AOR 7.28; 95% CI: 2.06-25.71).

Conclusions

The positive associations of distant incarceration with current opioid misuse and current opioid use disorder could indicate a persistent relationship between incarceration and substance use in people living with HIV and chronic pain. Additional research on opioid use among formerly incarcerated individuals in chronic pain treatment is needed.
Emotional risk factors before in vitro fertilization among infertile couples in daily clinical practice in Sari in 2020–2022
Authors: Kargar Sommayeh Taghaddosi , Vakili Fatemeh , Peivandi Sepideh , Jahanfar Shayesteh , Elyasi Forouzan , Hamzehgardeshi Zeinab
Published: May 2024

Background and Aim

The relationship between psychological factors and treatment outcomes with assisted reproductive technology has sparked considerable debate. This study aims to investigate the emotional risk factors in couples seeking infertility treatment using assisted reproductive technology in Sari, Iran, from 2020 to 2022.

Materials and methods

This research is a cross-sectional study and emotional risk factors and other related factors were examined using the Persian version of the SCREENIVF demographic, social, and clinical status questionnaire, social, and clinical status questionnaire before using Assisted reproductive technology in 460 infertile couples selected from infertility treatment centers in Sari City, Iran. The samples were randomly selected using a table of random numbers. Data analysis was performed using SPSS version 22 software.

Results

The mean age of the male and female participants were 31.70 ± 5.71 and 35.22 ± 5.48, respectively. The results regarding emotional risk factors and other related factors revealed that the variables of remarriage (P = 0.048) and exposure of spouse to emotional risk factors (P = 0.001), history of depression disorder (P = 0.007), and history of anxiety disorder (P = 0.009) were significantly correlated with the exposure of women to emotional risk factors. Furthermore, men’s exposure to emotional risk factors was significantly correlated with primary education (P = 0.026) and diploma (P = 0.043) levels, age (P = 0.006), and wife’s exposure to emotional risk factors (P = 0.001).

Conclusion

By identifying infertile couples who are at risk of emotional risk factors, healthcare professionals can provide appropriate support and interventions to mitigate the emotional challenges associated with infertility. This proactive approach can significantly enhance couples undergoing infertility treatment’s well-being and mental health.