bluuebunny/crossref_metadata_2025 huggingface dataset:
Benefits and Meaning of Lipids Profile in Relation to Oxidative Balance and Brain Morphology in Schizophrenia
Authors: Śmierciak Natalia , Krzyściak Wirginia , Szwajca Marta , Karcz Paulina , Bryll Amira , Popiela Tadeusz J. , Donicz Paulina , Turek Aleksander , Aleksandrovych Veronika , Pilecki Maciej
Published: July 2023

Schizophrenia is characterized by complex metabolic dysregulations and their consequences. Until now, numerous theories have explained its pathogenesis, using a spectrum of available technologies. We focused our interest on lipid profile—periphery high-density cholesterol level and lipoproteins in the human brain and compared magnetic resonance imaging (MRI) scans of patients with schizophrenia and the healthy group. Detailed analysis of biochemical parameters was performed using magnetic resonance spectroscopy. Our study aimed to reveal correlations between periphery high-density lipoproteins levels and lipoproteins in the brain, depicted in MRI scans, and parameters of peripheral oxidative stress expressed as paraoxonase. Patients with schizophrenia have decreased levels of high-density lipoproteins, low paraoxonase activity, and slightly raised sodium in the blood. Positive significant correlations between serum high-density cholesterol and anterior cingulate cortex, unique brain area for schizophrenia pathophysiology, MR spectroscopy signals, and diffusion have been revealed. To our knowledge, this is the first study to describe the effect of an anterior cingulate disorder on high-density cholesterol levels on the development of schizophrenia.
Moringa oleifera Lam. Isothiocyanate Quinazolinone Derivatives Inhibit U251 Glioma Cell Proliferation through Cell Cycle Regulation and Apoptosis Induction
Authors: Xie Jing , Yang Ming-Rong , Hu Xia , Hong Zi-Shan , Bai Yu-Ying , Sheng Jun , Tian Yang , Shi Chong-Ying
Published: July 2023

A major active constituent of Moringa oleifera Lam. is 4-[(α-L-rhamnose oxy) benzyl] isothiocyanate (MITC). To broaden MITC’s application and improve its biological activity, we synthesized a series of MITC quinazolinone derivatives and evaluated their anticancer activity. The anticancer effects and mechanisms of the compound with the most potent anticancer activity were investigated further. Among 16 MITC quinazolinone derivatives which were analyzed, MITC-12 significantly inhibited the growth of U251, A375, A431, HCT-116, HeLa, and MDA-MB-231 cells. MITC-12 significantly inhibited U251 cell proliferation in a time- and dose-dependent manner and decreased the number of EdU-positive cells, but was not toxic to normal human gastric mucosal cells (GES-1). Further, MITC-12 induced apoptosis of U251 cells, and increased caspase-3 expression levels and the Bax:Bcl-2 ratio. In addition, MITC-12 significantly decreased the proportion of U251 cells in the G1 phase and increased it in S and G2 phases. Transcriptome sequencing showed that MITC-12 had a significant regulatory effect on pathways regulating the cell cycle. Further, MITC-12 significantly decreased the expression levels of the cell cycle-related proteins CDK2, cyclinD1, and cyclinE, and increased those of cyclinA2, as well as the p-JNK:JNK ratio. These results indicate that MITC-12 inhibits U251 cell proliferation by inducing apoptosis and cell cycle arrest, activating JNK, and regulating cell cycle-associated proteins. MITC-12 has potential for use in the prevention and treatment of glioma.
Integrative Multi-Omics Analysis of Oncogenic EZH2 Mutants: From Epigenetic Reprogramming to Molecular Signatures
Authors: Aldana Julian , Gardner Miranda L. , Freitas Michael A.
Published: July 2023

Somatic heterozygous mutations in the active site of the enhancer of zeste homolog 2 (EZH2) are prevalent in diffuse large B-cell lymphoma (DLBCL) and acute myeloid leukemia (AML). The methyltransferase activity of EZH2 towards lysine 27 on histone H3 (H3K27) and non-histone proteins is dysregulated by the presence of gain-of-function (GOF) and loss-of-function (LOF) mutations altering chromatin compaction, protein complex recruitment, and transcriptional regulation. In this study, a comprehensive multi-omics approach was carried out to characterize the effects of differential H3K27me3 deposition driven by EZH2 mutations. Three stable isogenic mutants (EZH2Y641F, EZH2A677G, and EZH2H689A/F667I) were examined using EpiProfile, H3K27me3 CUT&Tag, ATAC-Seq, transcriptomics, label-free proteomics, and untargeted metabolomics. A discrete set of genes and downstream targets were identified for the EZH2 GOF and LOF mutants that impacted pathways involved in cellular proliferation, differentiation, and migration. Disruption of protein networks and metabolic signatures able to sustain aberrant cell behavior was observed in response to EZH2 mutations. This systems biology-based analysis sheds light on EZH2-mediated cell transformative processes, from the epigenetic to the phenotypic level. These studies provide novel insights into aberrant EZH2 function along with targets that can be explored for improved diagnostics/treatment in hematologic malignancies with mutated EZH2.
Assessing the Impact of Polyethylene Nano/Microplastic Exposure on Human Vaginal Keratinocytes
Authors: Pontecorvi Paola , Ceccarelli Simona , Cece Fabrizio , Camero Simona , Lotti Lavinia Vittoria , Niccolai Elena , Nannini Giulia , Gerini Giulia , Anastasiadou Eleni , Scialis Elena Sofia , Romano Enrico , Venneri Mary Anna , Amedei Amedeo , Angeloni Antonio , Megiorni Francesca , Marchese Cinzia
Published: July 2023

The global rise of single-use throw-away plastic products has elicited a massive increase in the nano/microplastics (N/MPLs) exposure burden in humans. Recently, it has been demonstrated that disposable period products may release N/MPLs with usage, which represents a potential threat to women’s health which has not been scientifically addressed yet. By using polyethyl ene (PE) particles (200 nm to 9 μm), we showed that acute exposure to a high concentration of N/MPLs induced cell toxicity in vaginal keratinocytes after effective cellular uptake, as viability and apoptosis data suggest, along with transmission electron microscopy (TEM) observations. The internalised N/MPLs altered the expression of junctional and adherence proteins and the organisation of the actin cortex, influencing the level of genes involved in oxidative stress signalling pathways and that of miRNAs related to epithelial barrier function. When the exposure to PE N/MPLs was discontinued or became chronic, cells were able to recover from the negative effects on viability and differentiation/proliferation gene expression in a few days. However, in all cases, PE N/MPL exposure prompted a sustained alteration of DNA methyltransferase and DNA demethylase expression, which might impact epigenetic regulation processes, leading to accelerated cell ageing and inflammation, or the occurrence of malignant transformation.
Roles of Lipopolysaccharide Glycosyltransferases in Maintenance of Helicobacter pylori Morphology, Cell Wall Permeability, and Antimicrobial Susceptibilities
Authors: Tang Xiaoqiong , Yang Tiankuo , Shen Yalin , Song Xiaona , Benghezal Mohammed , Marshall Barry J. , Tang Hong , Li Hong
Published: July 2023

Helicobacter pylori has a unique lipopolysaccharide structure that is essential in maintaining its cell envelope integrity and imbues the bacterium with natural resistance to cationic antimicrobial peptides (CAMPs). Our group has recently elucidated the complete set of LPS glycosyltransferase genes in H. pylori reference strain G27. Here, with a series of eight systematically constructed LPS glycosyltransferase gene mutants (G27ΔHP1578, G27ΔHP1283, G27ΔHP0159, G27ΔHP0479, G27ΔHP0102, G27ΔwecA, G27ΔHP1284 and G27ΔHP1191), we investigated the roles of H. pylori LPS glycosyltransferases in maintaining cell morphology, cell wall permeability, and antimicrobial susceptibilities. We demonstrated that deletion of these LPS glycosyltransferase genes did not interfere with bacterial cell wall permeability, but resulted in significant morphological changes (coccoid, coiled “c”-shape, and irregular shapes) after 48 h growth as compared to the rod-like cell shape of the wild-type strain. Moreover, as compared with the wild-type, none of the LPS mutants had altered susceptibility against clarithromycin, levofloxacin, amoxicillin, tetracycline, and metronidazole. However, the deletion of the conserved LPS glycosyltransferases, especially the O-antigen-initiating enzyme WecA, displayed a dramatic increase in susceptibility to the CAMP polymyxin B and rifampicin. Taken together, our findings suggest that the LPS glycosyltransferases play critical roles in the maintenance of the typical spiral morphology of H. pylori, as well as resistance to CAMPs and rifampicin. The LPS glycosyltransferases could be promising targets for developing novel anti-H. pylori drugs.
Enhanced Carotenoid Production in Chlamydomonas reinhardtii by Overexpression of Endogenousand Exogenous Beta-Carotene Ketolase (BKT) Genes
Authors: Chen Yuanhao , Du Hong , Liang Honghao , Hong Ting , Li Tangcheng
Published: July 2023

Chlamydomonas reinhardtii is a unicellular green alga that can grow heterotrophically by using acetate as a carbon source. Carotenoids are natural pigments with biological activity and color, which have functions such as antioxidant, anti-inflammatory, vision protection, etc., and have high commercial value and prospects. We transformed Chlamydomonas reinhardtii with the BKT genes from Phaffia rhodozyma (PrBKT) and Chlamydomonas reinhardtii (CrBKT) via plasmid vector, and screened out the stable transformed algal strains C18 and P1. Under the condition that the cell density of growth was not affected, the total carotenoid content of C18 and P1 was 2.13-fold and 2.20-fold higher than that of the WT, respectively. CrBKT increased the levels of β-carotene and astaxanthin by 1.84-fold and 1.21-fold, respectively, while PrBKT increased them by 1.11-fold and 1.27-fold, respectively. Transcriptome and metabolome analysis of C18 and P1 showed that the overexpression of CrBKT only up-regulated the transcription level of BKT and LCYE (the gene of lycopene e-cyclase). However, in P1, overexpression of PrBKT also led to the up-regulation of ZDS (the gene of ζ-carotene desaturase) and CHYB (the gene of β-carotene hydroxylase). Metabolome results showed that the relative content of canthaxanthin, an intermediate metabolite of astaxanthin synthesis in C18 and P1, decreased. The overall results indicate that there is a structural difference between CrBKT and PrBKT, and overexpression of PrBKT in Chlamydomonas reinhardtii seems to cause more genes in carotenoid pathway metabolism to be up-regulated.
Effects of Serotonin on Cell Viability, Permeability of Bovine Mammary Gland Epithelial Cells and Their Transcriptome Analysis
Authors: Lu Jie , Huang Guohao , Chang Xuan , Wei Bingni , Sun Yawang , Yang Zhengguo , Zhao Yongju , Zhao Zhongquan , Dong Guozhong , Chen Juncai
Published: July 2023

Serotonin (5-HT) has been reported to play an important role in mammary gland involution that is defined as the process through which the gland returns to a nonlactating state. However, the overall picture of the regulatory mechanisms of 5-HT and the effects of serotonylation on mammary gland involution still need to be further investigated. The current study aimed to investigate the effects of 5-HT on global gene expression profiles of bovine mammary epithelial cells (MAC-T) and to preliminarily examine whether the serotonylation involved in the mammary gland involution by using Monodansylcadaverine (MDC), a competitive inhibitor of transglutaminase 2. Results showed that a high concentration of 5-HT decreased viability and transepithelial electrical resistance (TEER) in MAC-T cells. Transcriptome analysis indicated that 2477 genes were differentially expressed in MAC-T cells treated with 200 μg/mL of 5-HT compared with the control group, and the Notch, p53, and PI3K-Akt signaling pathways were enriched. MDC influenced 5-HT-induced MAC-T cell death, fatty acid synthesis, and the formation and disruption of tight junctions. Overall, a high concentration of 5-HT is able to accelerate mammary gland involution, which may be regulated through the Notch, p53, and PI3K-Akt signaling pathways. Serotonylation is involved in bovine mammary gland involution.
The Role of the Intravenous IgA and IgM-Enriched Immunoglobulin Preparation in the Treatment of Sepsis and Septic Shock
Authors: Berlot Giorgio , Zanchi Silvia , Moro Edoardo , Tomasini Ariella , Bixio Mattia
Published: July 2023

Polyclonal Intravenous Immunoglobulins (IvIg) are often administered to critically ill patients more as an act of faith than on the basis of relevant clinical studies. This particularly applies to the treatment of sepsis and septic shock because the current guidelines recommend against their use despite many investigations that have demonstrated their beneficial effects in different subsets of patients. The biology, mechanisms of action, and clinical experience related to the administration of IvIg are reviewed, which aim to give a more in-depth understanding of their properties in order to clarify their possible indications in sepsis and septic shock patients.
Novel Biomarkers of Gastric Cancer: Current Research and Future Perspectives
Authors: Sato Yasushi , Okamoto Koichi , Kawano Yutaka , Kasai Akinari , Kawaguchi Tomoyuki , Sagawa Tamotsu , Sogabe Masahiro , Miyamoto Hiroshi , Takayama Tetsuji
Published: July 2023

Gastric cancer is a heterogeneous disease with diverse histological and genomic subtypes, making it difficult to demonstrate treatment efficacy in clinical trials. However, recent efforts have been made to identify molecular biomarkers with prognostic and predictive implications to better understand the broad heterogeneity of gastric cancer and develop effective targeted therapies for it. HER2 overexpression, HER2/neu amplification, MSI-H, and PD-L1+ are predictive biomarkers in gastric cancer, and a growing number of clinical trials based on novel biomarkers have demonstrated the efficacy of targeted therapies alone or in combination with conventional chemotherapy. Enrichment design clinical trials of targeted therapies against FGFR2b and claudin 18.2 have demonstrated efficacy in unresectable advanced gastric cancer. Nonetheless, it is essential to continuously validate promising molecular biomarkers and introduce them into clinical practice to optimize treatment selection and improve patient outcomes. In this review, we focused on established (PD-L1, HER2, MSI) and emerging biomarkers (FGFR2, CLDN18.2) in gastric cancer, their clinical significance, detection methods, limitations, and molecular agents that target these biomarkers.
Pb-210 Dating of Ice Scour in the Kara Sea
Authors: Kokin Osip , Usyagina Irina , Meshcheriakov Nikita , Ananiev Roman , Arkhipov Vasiliy , Kirillova Aino , Maznev Stepan , Nikiforov Sergey , Sorokhtin Nikolay
Published: July 2023

Ice scours are formed when the keels of floating icebergs or sea ice hummocks penetrate unlithified seabed sediments. Until now, ice scours have been divided into “relict” and “modern” according to the water depth that corresponds with the possible maximum vertical dimensions of the keels of modern floating icebergs. However, this approach does not consider climatic changes at the present sea level, which affect the maximum depth of ice keels. We present an application of 210Pb dating of the largest ice scour in the Baydaratskaya Bay area (Kara Sea), located at depths of about 28–32 m. Two sediment cores were studied; these were taken on 2 November 2021 from the R/V Akademik Nikolay Strakhov directly in the ice scour and on the “background” seabed surface, not processed via ice scouring. According to the results of 210Pb dating, the studied ice scour was formed no later than the end of the Little Ice Age. Based on the extrapolation of possible sedimentation rates prior to 1917 (0.22–0.38 cm/year), the age of the ice scour is estimated to be 1810 ± 30 AD. The mean rate of ice scour filling with 70 cm thick sediments from the moment of its formation is around 0.33 cm/year.
Mediterranean Aquaculture and Genetic Pollution: A Review Combined with Data from a Fish Farm Evaluating the Ecological Risks of Finfish Escapes
Authors: Alvanou Maria V. , Gkagkavouzis Konstantinos , Karaiskou Nikoleta , Feidantsis Konstantinos , Lattos Athanasios , Michaelidis Basile , Theodorou John A. , Batargias Costas , Triantafyllidis Alexandros , Giantsis Ioannis A.
Published: July 2023

Mediterranean finfish aquaculture is mainly represented by the production of the gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) growing in marine cage farms. Despite the numerous benefits of fish farming, the ecological risk potentially caused by fish escapes to the wild populations is occasionally high. In the current study, an integrated review of fish escapes is presented regarding S. aurata and D. labrax escapes and their potential effect on the genetic composition of wild populations. The collected data from the literature are combined and discussed along with recorded escapes in a fish farm located in the Aegean Sea, Greece. According to these records, fish escapes present a generally stable rate, ranging between 0 and 2% in each escape event, which occur 5–8 times per year. Although for other farmed fish species worldwide, the ecological risk may be higher, this risk is probably lower in Mediterranean finfish populations, which are characterized by generally low levels of genetic differentiation. Nevertheless, the risk of genetic introgression still exists. Particularly in sea bream and sea bass farming, genetic structure and differentiation in wild populations seem to be unaffected for now. Nevertheless, suitable management measurements would be a useful strategy to avoid future negative effects of fish escapes. These strategies should be focused on further efforts towards recapture program development, reduced escapees’ survival, and proper legislation. Furthermore, more data on escapees’ survival percentage, their migration pathways, and the way these pathways relate to mortality, the type of escape event, and the season of release would be also valuable.
Virtual Forest Environment Influences Inhibitory Control
Authors: Benedetti Viola , Gavazzi Gioele , Giganti Fiorenza , Carlo Elio , Becheri Francesco Riccardo , Zabini Federica , Giovannelli Fabio , Viggiano Maria Pia
Published: July 2023

Exposure to natural green environments, whether through visual media or in person, can bring numerous benefits to physical and mental health. Given the restorative effects that natural forest environments have on the human mind, it is plausible to assume that these effects can also extend to cognitive processes, such as cognitive control, which are fundamental to higher-level cognitive function. In this study, we investigated whether viewing videos of urban or forest environments would have an impact on inhibitory control and attention in people with or without a past COVID-19 infection. To investigate the impact of virtual natural and urban videos on cognitive performance, 45 participants were recruited, and the exposure to forest vs. urban videos was assessed on a Go/No-Go task and an Attentional Network Test. The data showed that in both groups, exposure to the forest videos improved the inhibitory component. The results are discussed in the context of the well-established evidence of the beneficial effects of green environments.
Spatiotemporal Variability in Soil Properties and Composition in Mangrove Forests in Baía de Todos os Santos (NE Brazil)
Authors: Vasconcelos Ramos Monica Arlinda , Pérez-Alberti Augusto , Nuto Nóbrega Gabriel , Otero Xosé Luis
Published: July 2023

Soil properties and components in mangrove ecosystems influence their geochemical processes and services. Despite the extensive mangrove areas present in Brazil, few studies focusing on these themes are under development. In this sense, this work aimed to investigate the spatial variability in soil attributes and composition, the geochemistry of Fe, and the isotopic characteristics of organic matter in mangroves in Baía de Todos os Santos (Cacha Prego, Ponta Grossa, Ilha de Maré, Pitinga), which constitutes Brazil’s second largest bay. The soils investigated showed spatial and temporal changes affecting their properties (pH, Eh) and composition (TOC, Fe fractions), as well as clear spatial changes in the redox potential values (+30–+188 mV), with higher values in PT. Soil textures ranged from predominantly sandy (CP, PT, PG: sand, >70%) to a finer granulometry (IM: sand, 33–64%). These characteristics influenced Fe partitioning and organic matter content, with higher TOC and pyrite values observed in IM (FeS2: 2720–9233 mg kg−1; TOC: 4.4–6.6%) and lower sulfide values found in PT, mainly in the dry season (FeS2: 85–235 mg kg−1). The soil δ13C and N/C ratios seem to suggest a mixed origin of organic matter.
Disparities in Female Oncofertility Care in the United States: More Questions Than Answers
Authors: Turner Kati A. , Spurlin Emily E. , Jimenez Patricia T.
Published: July 2023

As cancer therapies continue to improve, the survival rates of adolescent and young adult patients have increased. Consequently, considering patient quality of life after cancer, including family building, has become an essential aspect of establishing a treatment plan. However, the gonadotoxic nature of many chemotherapeutic agents limits the option of using one’s own gamete for family building. In recent years, significant advancements have been made in oncofertility, particularly vitrification of oocytes. Unfortunately, as with many areas of medicine, health disparities limit those that can access and utilize fertility preservation prior to cancer treatment. This review aims to shed light on existing disparities in oncofertility for female patients, to offer recommendations to enhance education, access, and advocacy, as well as identify potential areas for future research.
Assessment Model of Interactions Required in Design Teams in High-Rise Building Projects
Authors: Herrera Rodrigo F. , Galaz-Delgado Eduardo I. , Atencio Edison , Muñoz-La Rivera Felipe , Castillo Tito
Published: July 2023

There is a lack of knowledge of the interactions required over time among the members of a building project design team. Without a specific target, it is impossible to identify gaps to propose improvement plans in the coordination and management of projects in the early stages. Therefore, this study proposes a model of the required interaction among the members of a building project design team during the different phases of the design process. The research was divided into three stages: (1) design team interactions; (2) construction of ideal interaction networks and proposed evaluation model; and (3) pilot cases—evaluation and analysis. Through this study, eight ideal networks were constructed, four for each interaction (information flow, collaboration, and coordination) and one for each design phase. In addition, a series of metrics were proposed to evaluate the current state of a building project, which, together with the constructed model networks, allowed the development of an evaluation method for real projects. Finally, two pilot cases were used to exemplify the use of the proposed model tool.
An Effective Approach Based on Generalized Bernstein Basis Functions for the System of Fourth-Order Initial Value Problems for an Arbitrary Interval
Authors: Basit Muhammad , Shahnaz Komal , Malik Rida , Karim Samsul Ariffin Abdul , Khan Faheem
Published: July 2023

The system of ordinary differential equations has many uses in contemporary mathematics and engineering. Finding the numerical solution to a system of ordinary differential equations for any arbitrary interval is very appealing to researchers. The numerical solution of a system of fourth-order ordinary differential equations on any finite interval [a,b] is found in this work using a symmetric Bernstein approximation. This technique is based on the operational matrices of Bernstein polynomials for solving the system of fourth-order ODEs. First, using Chebyshev collocation nodes, a generalised approximation of the system of ordinary differential equations is discretized into a system of linear algebraic equations that can be solved using any standard rule, such as Gaussian elimination. We obtain the numerical solution in the form of a polynomial after obtaining the unknowns. The Hyers–Ulam and Hyers–Ulam–Rassias stability analyses are provided to demonstrate that the proposed technique is stable under certain conditions. The results of numerical experiments using the proposed technique are plotted in figures to demonstrate the accuracy of the specified approach. The results show that the suggested Bernstein approximation method for any interval is quick and effective.
Transformer Models and Convolutional Networks with Different Activation Functions for Swallow Classification Using Depth Video Data
Authors: Lai Derek Ka-Hei , Cheng Ethan Shiu-Wang , So Bryan Pak-Hei , Mao Ye-Jiao , Cheung Sophia Ming-Yan , Cheung Daphne Sze Ki , Wong Duo Wai-Chi , Cheung James Chung-Wai
Published: July 2023

Dysphagia is a common geriatric syndrome that might induce serious complications and death. Standard diagnostics using the Videofluoroscopic Swallowing Study (VFSS) or Fiberoptic Evaluation of Swallowing (FEES) are expensive and expose patients to risks, while bedside screening is subjective and might lack reliability. An affordable and accessible instrumented screening is necessary. This study aimed to evaluate the classification performance of Transformer models and convolutional networks in identifying swallowing and non-swallowing tasks through depth video data. Different activation functions (ReLU, LeakyReLU, GELU, ELU, SiLU, and GLU) were then evaluated on the best-performing model. Sixty-five healthy participants (n = 65) were invited to perform swallowing (eating a cracker and drinking water) and non-swallowing tasks (a deep breath and pronouncing vowels: “/eɪ/”, “/iː/”, “/aɪ/”, “/oʊ/”, “/u:/”). Swallowing and non-swallowing were classified by Transformer models (TimeSFormer, Video Vision Transformer (ViViT)), and convolutional neural networks (SlowFast, X3D, and R(2+1)D), respectively. In general, convolutional neural networks outperformed the Transformer models. X3D was the best model with good-to-excellent performance (F1-score: 0.920; adjusted F1-score: 0.885) in classifying swallowing and non-swallowing conditions. Moreover, X3D with its default activation function (ReLU) produced the best results, although LeakyReLU performed better in deep breathing and pronouncing “/aɪ/” tasks. Future studies shall consider collecting more data for pretraining and developing a hyperparameter tuning strategy for activation functions and the high dimensionality video data for Transformer models.
(ω,ρ)-BVP Solutions of Impulsive Differential Equations of Fractional Order on Banach Spaces
Authors: Fečkan Michal , Kostić Marko , Velinov Daniel
Published: July 2023

The paper focuses on exploring the existence and uniqueness of a specific solution to a class of Caputo impulsive fractional differential equations with boundary value conditions on Banach space, referred to as (ω,ρ)-BVP solution. The proof of the main results of this study involves the application of the Banach contraction mapping principle and Schaefer’s fixed point theorem. Furthermore, we provide the necessary conditions for the convexity of the set of solutions of the analyzed impulsive fractional differential boundary value problem. To enhance the comprehension and practical application of our findings, we conclude the paper by presenting two illustrative examples that demonstrate the applicability of the obtained results.
Bio-Inspired Iron-Loaded Polydopamine Functionalized Montmorillonite as an Environmentally Friendly Flame Retardant for Epoxy Resin
Authors: Lan Jiashui , Li Dingsi , Zhong Wei , Luo Wenhui , Zhang Huagui , Chen Mingfeng
Published: July 2023

As an important thermosetting material, flame-retardant epoxy resin has various applications in the aerospace, chemical, and electronics industry, and other fields. However, the flame retardancy of epoxy resins is often improved at the expense of mechanical performance. The contradiction between flame retardancy and mechanical properties seriously impedes the practical applications of epoxy resin (EP). Herein, iron-loaded polydopamine functionalized montmorillonite (D-Mt-Fe3+), which was prepared by dopamine, iron chloride and montmorillonite in an aqueous solution, was introduced to prepare iron-loaded polydopamine functionalized montmorillonite/epoxy resin composites (D-Mt-Fe3+/EP). As expected, D-Mt-Fe3+/EP-10 with 10 phr of D-Mt-Fe3+ passed the UL-94 V-0 rating, achieved a limiting oxygen index (LOI) value of 31.0% and reduced the smoke production rate (SPR) and total smoke production (TSP), indicating that the introduction of D-Mt-Fe3+ could endow EP with satisfactory flame retardancy through the radical scavenging function of dopamine in the gas phase and the catalytic charring effect of iron ions, respectively. Encouragingly, the mechanical property was also enhanced with the flexural strength increased by 25.5%. This work provided an attractive strategy for improving both the mechanical properties and fire resistance of EP, which greatly broadened their applications in the chemical industry and electronics field, etc.
The Importance of the Pyrazole Scaffold in the Design of Protein Kinases Inhibitors as Targeted Anticancer Therapies
Authors: Nitulescu George Mihai , Stancov Gheorghe , Seremet Oana Cristina , Nitulescu Georgiana , Mihai Dragos Paul , Duta-Bratu Cosmina Gabriela , Barbuceanu Stefania Felicia , Olaru Octavian Tudorel
Published: July 2023

The altered activation or overexpression of protein kinases (PKs) is a major subject of research in oncology and their inhibition using small molecules, protein kinases inhibitors (PKI) is the best available option for the cure of cancer. The pyrazole ring is extensively employed in the field of medicinal chemistry and drug development strategies, playing a vital role as a fundamental framework in the structure of various PKIs. This scaffold holds major importance and is considered a privileged structure based on its synthetic accessibility, drug-like properties, and its versatile bioisosteric replacement function. It has proven to play a key role in many PKI, such as the inhibitors of Akt, Aurora kinases, MAPK, B-raf, JAK, Bcr-Abl, c-Met, PDGFR, FGFRT, and RET. Of the 74 small molecule PKI approved by the US FDA, 8 contain a pyrazole ring: Avapritinib, Asciminib, Crizotinib, Encorafenib, Erdafitinib, Pralsetinib, Pirtobrutinib, and Ruxolitinib. The focus of this review is on the importance of the unfused pyrazole ring within the clinically tested PKI and on the additional required elements of their chemical structures. Related important pyrazole fused scaffolds like indazole, pyrrolo[1,2-b]pyrazole, pyrazolo[4,3-b]pyridine, pyrazolo[1,5-a]pyrimidine, or pyrazolo[3,4-d]pyrimidine are beyond the subject of this work.
Progress on the Extraction, Separation, Biological Activity, and Delivery of Natural Plant Pigments
Authors: Lu Xianwen , Li Wenjun , Wang Qi , Wang Jing , Qin Song
Published: July 2023

Natural plant pigments are safe and have low toxicity, with various nutrients and biological activities. However, the extraction, preservation, and application of pigments are limited due to the instability of natural pigments. Therefore, it is necessary to examine the extraction and application processes of natural plant pigments in detail. This review discusses the classification, extraction methods, biological activities, and modification methods that could improve the stability of various pigments from plants, providing a reference for applying natural plant pigments in the industry and the cosmetics, food, and pharmaceutical industries.
Ex Vivo Perfusion of Porcine Pancreas and Liver Sourced from Commercial Abattoirs after Circulatory Death as a Research Resource: A Methodological Study
Authors: Rai Zainab L. , Magbagbeola Morenike , Doyle Katie , Lindenroth Lukas , Dwyer George , Gander Amir , Stilli Agostino , Stoyanov Danail , Davidson Brian R.
Published: July 2023

Background: Machine perfusion (MP) is increasingly used for human transplant organ preservation. The use of MP for research purposes is another opportunity for this technology. The porcine pancreas and liver are similar in anatomical size and function to their human counterparts, making them an excellent resource for research, but they have some important differences from human organs which can influence their research use. In this paper, we describe a technique developed and tested for the retrieval of porcine organs for use in research on perfused viable organs. Methods: Whole-organ porcine pancreata and livers were harvested at a commercial abattoir, following standard slaughterhouse processes. The standard slaughterhouse process involved a thoracotomy and mid-line laparotomy, and all the thoracoabdominal organs were removed. The pancreas, fixed in the retroperitoneum, was carefully dissected from its attachments to the surrounding structures, and tissue planes between the pancreas, spleen, duodenum, and colon were meticulously identified and dissected. Vessel exposure and division: The aorta, portal vein (PV), hepatic vein (HV), and hepatic artery (HA) were dissected and isolated, preserving the input and output channels for the liver and pancreas. A distal 3 cm of the aorta was preserved and divided and served as the input for the pancreas perfusions. The liver, PV, HV, and HA were preserved and divided to preserve the physiological channels of the input (PV and HA) and output (HV) for the liver perfusions. The porcine hepatic and pancreas anatomy shares significant resemblance with the gross anatomy found in humans, and this was taken into consideration when designing the perfusion circuitry. The porcine pancreas and spleen shared a common blood supply, with branches arising from the splenic artery. The organs were flushed with cold, heparinised normal saline and transported in a temperature-regulated receptacle maintained at a core temperature between 4 and 8 °C, in line with the standards of static cold storage (SCS), to a dedicated perfusion lab and perfused using our novel perfusion machine with autologous, heparinised porcine blood, also collected at the abattoir.
An Overview of Some Reactive Routes to Flame-Retardant Fibre-Forming Polymers: Polypropylene and Polyacrylonitrile
Authors: Tretsiakova-McNally Svetlana , Arun Malavika , Guerrieri Maurice , Joseph Paul
Published: July 2023

The thermal degradation and flammability characteristics of some common fibre-forming polymers, such as polypropylene (PP) and polyacrylonitrile (PAN), are described in this review paper. The flame retardance of these polymers is principally affected by reactive routes that were primarily developed in our laboratories. The modifying groups that are incorporated into polymeric chains include phosphorus- or phosphorus/nitrogen-containing moieties in different chemical environments. The degradation characteristics and extent of flame retardance were mainly evaluated using routine thermal and calorimetric techniques. Elements of flame-retardant mechanisms occurring in the condensed and vapour phases were also identified. Furthermore, we also explored the effects of molecularly dispersed β-cyclodextrin, including its physical mixtures, on the thermal and combustion characteristics of PAN. Given that both types of polymers are often used in the form of fibres, and that the aspect ratio of fibrous materials is relatively high, even nominal enhancements in their fire retardance are highly welcomed. Hence, the preliminary results of our research on chemically modified PAN incorporating molecularly dispersed β-cyclodextrin are encouraging in terms of their enhanced fire retardance, and hence this field warrants further exploration.
High-Speed Underwater Optical Wireless Communication with Advanced Signal Processing Methods Survey
Authors: Fang Chengwei , Li Shuo , Wang Yinong , Wang Ke
Published: July 2023

Underwater wireless communication (UWC) technology has attracted widespread attention in the past few years. Compared with conventional acoustic underwater wireless communication technology, underwater optical wireless communication (UOWC) technology has promising potential to provide high data rate wireless connections due to the large license-free bandwidth. Building a high-performance and reliable UOWC system has become the target of researchers and various advanced and innovative technologies have been proposed and investigated. Among them, better hardware such as transmitters and receivers, as well as more advanced modulation and signal processing techniques, are key factors in improving UOWC system performance. In this paper, we review the recent development in UOWC systems. In particular, we provide a brief introduction to different types of UOWC systems based on channel configuration, and we focus on various recent studies on advanced signal processing methods in UOWC systems, including both traditional non-machine learning (NML) equalizers and machine learning (ML) schemes based on neural networks. In addition, we also discuss the key challenges in UOWC systems for future applications.
Indoor Visible Light Positioning Based on Channel Estimation and Cramér–Rao Low Bound Analysis with Random Receiving Orientation of User Equipment
Authors: Deng Lijun , Fan Yangyu , Zhao Qiong , Wu Pengfei
Published: July 2023

The accuracy of line-of-sight (LOS)-based indoor visible light positioning (VLP) depends directly on the accuracy of the received LOS power. In an actual indoor visible light communication (VLC) system, the total received power contains the LOS power and diffuse power, and the proportion of diffuse component varies significantly with the receiving orientation of the user equipment (UE). In the trilateration positioning process, this is bound to bring a very high level of inaccuracy for at least one or two of them when determining the distance between the positioned LED and UE. To solve this problem, we employed channel estimation and Parseval’s theorem to remove the diffuse component from the total received power. The simulation results show that the higher the channel estimation accuracy, the smaller the discrepancy between the estimated and actual LOS power, and the greater the localization accuracy. At the same channel estimation accuracy, the comparison results demonstrate that the localization performance of the proposed method is higher than LLS-1, in which the total received power is directly adopted to estimate the distance. The localization performance of LLS-2 deteriorates significantly compared to the proposed method. This result indicates that the receiving orientation cannot be neglected during the distance estimation process even though the estimated accuracy of LOS power is high. Deriving the Cramér–Rao low bound (CRLB) for LOS-based indoor VLP with receiving orientation, we compared the localization performance for the proposed method with LLS-1, LLS-2, and the CRLB at various signal-to-noise ratios (SNRs). The results show that the localization error of all the received positions in the room can reach the CRLB at approximately 25 dB. However, localization errors based on LLS-1 and LLS-2 cannot achieve the theoretical CRLB even at a high SNR.
Molecular Mechanism Underlying the Sorghum sudanense (Piper) Stapf. Response to Osmotic Stress Determined via Single-Molecule Real-Time Sequencing and Next-Generation Sequencing
Authors: Liu Qiuxu , Wang Fangyan , Xu Yalin , Lin Chaowen , Li Xiangyan , Xu Wenzhi , Wang Hong , Zhu Yongqun
Published: July 2023

Drought, as a widespread environmental factor in nature, has become one of the most critical factors restricting the yield of forage grass. Sudangrass (Sorghum sudanense (Piper) Stapf.), as a tall and large grass, has a large biomass and is widely used as forage and biofuel. However, its growth and development are limited by drought stress. To obtain novel insight into the molecular mechanisms underlying the drought response and excavate drought tolerance genes in sudangrass, the first full-length transcriptome database of sudangrass under drought stress at different time points was constructed by combining single-molecule real-time sequencing (SMRT) and next-generation transcriptome sequencing (NGS). A total of 32.3 Gb of raw data was obtained, including 20,199 full-length transcripts with an average length of 1628 bp after assembly and correction. In total, 11,921 and 8559 up- and down-regulated differentially expressed genes were identified between the control group and plants subjected to drought stress. Additionally, 951 transcription factors belonging to 50 families and 358 alternative splicing events were found. A KEGG analysis of 158 core genes exhibiting continuous changes over time revealed that ‘galactose metabolism’ is a hub pathway and raffinose synthase 2 and β-fructofuranosidase are key genes in the response to drought stress. This study revealed the molecular mechanism underlying drought tolerance in sudangrass. Furthermore, the genes identified in this study provide valuable resources for further research into the response to drought stress.
Topography of UV-Melanized Thalli of Lobaria pulmonaria (L.) Hoffm
Authors: Daminova Amina G. , Rassabina Anna E. , Khabibrakhmanova Venera R. , Beckett Richard P. , Minibayeva Farida V.
Published: July 2023

Lichens are unique extremophilic organisms due to their phenomenal resistance to adverse environmental factors, including ultraviolet (UV) irradiation. Melanization plays a special role in the protection of lichens from UV-B stress. In the present study, we analyzed the binding of melanins with the components of cell walls of the mycobiont of the upper cortex in the melanized lichen thalli Lobaria pulmonaria. Using scanning electron and atomic force microscopy, the morphological and nanomechanical characteristics of the melanized layer of mycobiont cells were visualized. Melanization of lichen thalli led to the smoothing of the surface relief and thickening of mycobiont cell walls, as well as the reduction in adhesion properties of the lichen thallus. Treatment of thalli with hydrolytic enzymes, especially chitinase and lichenase, enhanced the yield of melanin from melanized thalli and promoted the release of carbohydrates, while treatment with pectinase increased the release of carbohydrates and phenols. Our results suggest that melanin can firmly bind with hyphal cell wall carbohydrates, particularly chitin and 1,4-β-glucans, strengthening the melanized upper cortex of lichen thalli, and thereby it can contribute to lichen survival under UV stress.
What Is Wrong with Frankenia nodiflora Lam. (Frankeniaceae)? New Insights into the South African Sea-Heaths
Authors: Crespo Manuel B. , Alonso María Ángeles , Martínez-Azorín Mario , Villar José Luis , Mucina Ladislav
Published: July 2023

The taxonomic identity and phylogenetic relationships of several southern African perennial taxa related to Frankenia repens are discussed. In particular, F. nodiflora Lam., a misunderstood species described from the Cape region and synonymised to F. pulverulenta, is restored for plants endemic to salt-pans and riverbeds in the coastal lowlands across the Cape Flats (Western Cape province, South Africa). Further, a revision of morphologically close plants, usually identified as F. pulverulenta or F. repens, also occurring in similar saline ecosystems of the inland western South Africa revealed the existence of two distinct new entities not matching any described taxa of the genus. Molecular analyses of nuclear ribosomal (ITS1-5.8S-ITS2 region) DNA sequence data together with morphological divergence allow recognition of those taxa at species rank, within an independent lineage close to F. repens. In consequence, two new sea-heath species are described in the so-called “F. repens group”: F. nummularia from the Nama-Karoo Biome (Western Cape and Northern Cape provinces), and F. anneliseae from the Succulent Karoo Biome (Northern Cape province). Full morphological description and type designation are reported for each accepted species as well as data on ecology, habitat, distribution, and taxonomic relationships to other close relatives are given. Further, an identification key is presented to facilitate recognition of the southern African taxa of Frankenia.
Post Acid Treatment on Pressurized Liquid Extracts of Sorghum (Sorghum bicolor L. Moench) Grain and Plant Material Improves Quantification and Identification of 3-Deoxyanthocyanidins
Authors: Santana Ádina L. , Peterson Jaymi , Perumal Ramasamy , Hu Changling , Sang Shengmin , Siliveru Kaliramesh , Smolensky Dmitriy
Published: July 2023

Sorghum is a unique natural food source of 3-deoxyanthocyanidins (3-DA) polyphenols. This work evaluated the effect of acidification on sorghum extracts post pressurized liquid extraction (PLE) and its ability to increase the identification and quantification of 3-DA. The sorghum genotypes included Sumac and PI570366 (bran only) and SC991 (leaf and leaf sheath tissue). The acidification of the PLE extracts was carried out with methanol–HCl solutions at various concentrations (0, 0.5, 1, 2, and 4%, v/v). Changes in color were determined using L*a*b*. The overall phenolic composition was estimated with the total phenolic content and the DPPH free radical scavenging assays. Quantitative and qualitative chromatographic methods determined the phenolic profile. Color analysis showed that the redness and color saturation increased after acidification. No statistical difference was found in the total phenolic content of the acidified extracts, except for SC991, which was increased. There were no differences in the antioxidant capacity following acidification in all samples. For chromatographic analysis, luteolinidin was predominant in the extracts and the 3-DA content increased after acid treatment. However, some flavonoid and phenolic acid concentrations decreased following acid treatment, including taxifolin, quercetin, and chlorogenic acid. Interestingly, 0.5% v/v HCl acidification was sufficient to increase the color, allow the detection of 5-methoxyluteolinidin, and to increase luteolinidin and 7-methoxyapigenidin by at least twofold.
Analysis of Vortex Evolution in the Runner Area of Water Pump Turbine under Runaway Conditions
Authors: Zhou Feng , Li Qifei , Xin Lu
Published: July 2023

In order to study the evolution principle of the coherent structure in the low flow rate runaway condition, the pump turbine of a certain pumped storage power plant was employed. The transient dynamic stress of the runner was numerically simulated and examined in this study in order to analyze the coherent structure of the vortex and the stability of the grid connection during the transition process. Based on the realizable k-ε turbulent model, the unsteady flow of the whole pump turbine channels was calculated. The results show that the flow in the runner channels presents with a turbulence state, and with many different scales vortices. These vortex structures are mainly distributed in the inlet region of the blade, the area of the blade trailing edge and the middle section of the runner channels. These vortex structures affect the distribution of the blade pressure load. Moreover, the vortex structure at the inlet of the runner depends on the change in the attack angle. In the flow region formed at the outlet of the blade near the suction surface and the runner cone, the blade has a limited effect on the fluid; thus, the vortex structure depends on the Coriolis force and the centrifugal force joint action. The evolution of these vortex structures will have a greater impact on the grid connection of the power station. During the operation of the power station, it is necessary to reduce the time of transitional conditions so that the power station can operate efficiently.