bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Tempat Pembuangan Akhir(TPA) Terhadap Pencemaran Udara Di lingkungan Sebakul Kota Bengkulu
Authors: Sari Putri Della Verta , Nyai Sakti Hesti Putri , Walid Ahmad
Published: March 2022

Tempat Pembuangan Akhir ( TPA) merupakan salah satu tempat yang digunakan untuk membuang sampah yang sudah mencapai tahap akhir dalam pengelolaan sampah yang dimulai dari pertama kali sampah dihasilkan, dikumpulkan, diangkut , dikelola dan dibuang. pencemaran udara adalah perusak terhadap kualitas udara. Kerusakan kualitas ini disebabkan oleh berbagai sumber, baik sumber biologis maupun nonbiologis. Polusi udara dapat bersumber dari berbagai macam antara lain, asap kendaraan bermotor, asap pabrik, limbah industry, limbah rumah tangga dan lain-lain. Maka Tujuan dari penelitian ini Untuk mengetahui hubungan pengaruh antara tempat pembuangan akhir(TPA) dengan tejadinya pencemaran udara di air sebakul kota Bengkulu. Jenis penelitian bersifat studi pustaka, field study dan dokumentasi dilokasi terjadinya pencemaran udara. Hasil penelitian pencemaran udara diakibatkan oleh TPA tersebut yang menghasilkan bau menyegat pada udara yang telah membuat pencemaran udara dan memiliki dampak terhadap pemukiman warga sekitar. Sampah yang telah mengeluarkan gas karbon yang membuat terjadinya pencemaran udara.Kata kunci: TPA, Pencemaran udara
Penguatan Desa Siaga COVID 19
Authors: Mukhsar Mukhsar , Usman Ida , Abapihi Bahriddin , Sani Asrul , Baharuddin Baharuddin
Published: March 2022

COVID-19 adalah penyakit yang sangat menular dan masih menjadi masalah serius di seluruh dunia. Data kasus Propinsi Sulawesi Tenggara menunjukkan peningkatan signifikan dan sudah sampai ke pelosok desa. Untuk menghambat penyebaran virus ini adalah tetap tinggal di rumah jika tidak ada kepentingan mendesak. Jika harus keluar rumah maka harus mematuhi protocol kesehatan. Diperlukan penguatan desa agar masyaBahriddin Abapihirakatnya tidak terpapar COVID 19 dengan memberikan sosialisasi tentang COVID-19. Untuk itu perlu pengukuran pengetahuan masyakarat agar untuk menentukan metode sosialisasi yang tepat. Pengukuran dilakukan dua kali di 27 desa dengan jumlah responden sebanyak 880 orang, 51,02% perempuan dan sisanya adalah laki-laki. Jumlah responden dengan pekerjaan petani (19,66%) lebih banyak dibandingkan jumlah responden dengan jenis pekerjaan ASN, pedagang, nelayan, pelajar, buruh, tukang ojek, penjaga toko, dan ibu rumah tangga. Sementara itu, jenjang pendidikan SMA atau sederajat (39,55%) dengan jenjang pendidikan lainnya hampir berimbang. Hasil pengukuran pada tahap awal menunjukkan bahwa sebesar 62,29% mengetahui COVID 19 dan sebesar 48,96% msayarakat melakukan tindakan pencegahan. Setelah dilakukan sosialisasi selama 4 minggu, menunjukkan bahwa pengetahuan, sikap, dan perilaku atau tindakan, masing-msing mengalami peningkatan sebesar 93,33%, 97,05%, dan 94,44%. Terjadi kesadaran tentang pencegahan COVID 19.Kata Kunci: Covid 19, Desa Siaga, Pengetahuan, Sikap dan Tindakan
Penerapan Inovasi Tirai Isolasi Mandiri Pasien Covid-19
Authors: Mangalla Lukas K. , Simatupang Minson , Samhuddin Samhuddin , Kadir Kadir , Kadir Abd.
Published: March 2022

The world is currently facing the biggest problem on new corona virus diseases called Covid-19. The rapid increase in the number of cases has challenged governments and experts to provide solutions for a lot patients infected by this virus such as medical treatments and health infrastructures. One of the effort to break the chain of transmission of the virus has been made by lecturers and students of Universitas Halu Oleo through the community service program. This program aims to increase the awareness of the community during the outbreak of Covid-19 as well as to introduce an important health infrastructure device called Independent Isolation Curtain for Covid-19 Patient (TIMPA Covid-19). This device can be used at home to localize the droplet spreading from patients when coughing or sneezing. The method of implementing this program consists of enlightened the community as well as educated the people on how to prevent their family from Covid-19 transmissions. The activity was also provided the device TIMPA Covi-19 model as well as showed the instruction of using it at home. This activity was conducted at Balai Desa Pambulaa Jaya Konawe Selatan with involving the village officials and all community members. The result of this program shows that the community have a good understanding and well improvement of knowledge on the basic rules of Covid-19 protection and how to isolate independently the patients of Covid-19 at home. In addition, the community can also understand how to make simple and affordable devices for self-isolation equipment of covid-19 patient.Kata Kunci: Curtain, Independent isolation, Prevention, Patient and Covid-19
Peningkatan Pendapatan Peternak Ayam Kampung Melalui Penerapan Teknologi IB dan Mesin Tetas di Kelurahan Lalodati Kecamatan Puuwatu Kota Kendari
Authors: Abadi Musram , Nafiu La Ode , Libriani Restu , Kimestri Asma Bio , Badaruddin Rusli
Published: March 2022

AbstrakAyam kampung merupakan unggas penghasil daging dan telur yang cukup populer di Kota Kendari. Selain untuk memenuhi kebutuhan dasar keluarga akan produk hewani, usaha ternak ayam kampung juga dapat dijadikan sebagai sumber tambahan pendapatan bagi keluarga. Salah satu kelurahan yang potensial untuk pengembangan ayam kampung di Kota Kendari adalah Kelurahan Lalodati Kecamatan Puuwatu. Di Kelurahan Lalodati Kecamatan Puuwatu terdapat Kelompok peternak ayam kampong namun Sistem pemeliharaan yang dilakukan oleh peternak secara umum masih ektensif dan semi intensif. Penerapan teknologi tepat guna belum dimanfaatkan secara maksimal oleh peternak. Hal tersebut menjadi kendala dalam pengembangan usaha ternak ayam kampung, yang cenderung stagnan atau bahkan mengalami penurunan produksi. Sehingga diperlukan upaya peningkatan pengetahuan dan keterampilan peternak dalam mengembangkan usaha ternak ayam kampungnya melalui Program Kemitraan Masyarakat Internal (PKM-I) UHO. PKMI-UHO ini dilaksanakan selama tiga bulan yaitu dimulai sejak September-November 2020 di Kelurahan Lalodati Kecamatan Puuwatu Kota Kendari. Pelaksanaan program dilakukan dalam bentuk penyuluhan dan bimbingan teknis dengan metode tatap muka baik secara individu, kelompok, ataupun massal dengan tetap memperhatikan protokol pencegahan Covid-19. Adapun materi penyuluhan program pemberdayaan ini, yaitu konsep dasar aplikasi inseminasi buatan (IB) pada ayam kampung dan manajemen penetasan menggunakan mesin tetas buatan. Berdasarkan hasil pelaksanaan kegiatan pengabdian pada masyarakat melalui Program Kemitraan Masyarakat Internal (PKM-I) UHO dengan pendekatan penyuluhan dan bimbingan teknis pada kelompok peternak pembudidaya ayam kampung di Kelurahan Lalodati Kecamatan Puwaatu Kota Kendari dapat meningkatkan pengetahuan dan motivasi peternak pembudidaya ayam kampung tentang manajemen sistem pemuliaan ternak ayam kampung dan meningkatkan keterampilan peternak dalam penyusunan pakan fermentasi.
Membentuk Kesadaran Hukum Masyarakat Untuk Vaksinasi Covid-19 Melalui Penyuluhan Hukum di Desa Wonua Morini Kecamatan Morosi Kabupaten Konawe
Authors: Hasima Rahman , Faisal Fitriah , Isnayanti Isnayanti
Published: March 2022

Beberapa masalah yang dialami oleh mitra yaitu sebagai berikut: (1) kurang patuhnya masyarakat terhadap himbauan dari pemerintah tentang pentingnya vaksinasi Covid-19, (2) kurangnya minat baca dari masyarakat perihal program vaksinasi Covid-19, (3) kurangnya kesadaran pribadi dari masyarakat itu sendiri betapa pentingnya edukasi terkait vaksinasi Covid-19 dalam rangka penanggulangan Covid-19, (4) kurangnya sosialisasi dan edukasi yang didapat masyarakat karena hanya mendengarkan dana membaca berita di televisi dan media sosial. Solusi yang ditawarkan adalah mengadakan penyuluhan hukum kepada masyarakat di desa mitra mengenai kebijakan yang mengatur pelaksanaan vaksinasi Covid-19 agar program vaksinasi Covid-19 pada seluruh lapisan masyarakat dapat terlaksana dengan baik. Tujuan dari kegiatan pengabdian ini adalah tujuan kegiatan pengabdian kepada masyarakat adalah untuk membentuk kesadaran hukum masyarakat untuk melaksanakan vaksinasi Covid-19 melalui penyuluhan hukum dalam rangka penanggulangan Covid-19. Hasil kegiatan pengabdian ini menunjukan bahwa dengan adanya penyuluhan hukum dapat meningkatkan kesadaran hukum masyarakat Desa Wonua Morini terkait dengan pentingnya vaksinasi covid-19 yang berdasarkan hasil evaluasi pelaksanaan kegiatan pengabdian bahwa adanya perbedaan kesadaran hukum masyarakat sebelum dan sesudah dilaksanakan kegiatan program kemitraan masyarakat internal yaitu sebesar 50% sehingga berdampak pada terbentuknya kesadaran hukum masyarakat terhadap pentingnya vaksinasi covid-19 dalam rangka pencegahan penularan covid-19.
Penataan Desa Berbasis Teknologi Informasi Pada Desa Tanjung Tiram Kecamatan Moramo Utara
Authors: Djafar Muh. Kabil , Sani Asrul , Somayasa Wayan , Jufra Jufra , Mukhtar Norma , Budiman Herdi
Published: March 2022

Pelaksanaan program kegiatan Pengabdian kepada Masyarakat Terintegrasi Kuliah Kerja Nyata– Tematik (KKN-Tematik) bertujuan untuk meningkatkan efisiensi pelayanan masyarakat, dengan memanfaatkan teknologi informasi dalam bentuk Sistem Informasi Desa bebasis web. Metode pelaksanaan kegiatan ini dengan caramemberikan pelatihan teknologi informasi secara formal dan nonformal kepada masyarakat dan perangkat desa. Hasil dari kegiatan PkM terintegrasi KKNTematik adalah mitra dalam hal ini Desa Tanjung Tiram memiliki Sistem Informasi Desa/Website. Pengelolaan Sistem Informasi Desa dilaksanakan oleh seorang perangkat desa, yang telah mendapat pelatihan teknologi informasi.
Bimbingan Teknis Budidaya Tanaman Hortikultura Di Desa Jati Bali Kabupaten Konawe Selatan
Authors: Sutariati Gusti Ayu Kade , Safuan La Ode , Leomo Siti , Rahni Nini Mila , Wibawa Gusti Ngurah Adhi
Published: March 2022

Tanaman hortikultura (sayuran-buah) organik merupakan sumber vitamin dan mineral yang sangat dibutuhkan untuk meningkatkan daya tahan tubuh terhadap berbagai penyakit. Program Kemitraan Masyarakat Internal (PKMI) yang dilaksanakan dalam bentuk Pengabdian kepada Masyarakat ini bertujuan mendekatkan teknologi budidaya tanaman sayuran-buah secara organik kepada masyarakat sasaran yaitu Kelompok Ibu-ibu Wanita Hindu Dharma Indonesia (WHDI) di Desa Jati Bali Kabupaten Konawe Selatan, sehingga mereka dapat mengembangkan secara mandiri teknik ini di pekarangan rumah masing-masing. Metode pendekatan yang digunakan adalah penyuluhan, pelatihan dan bimbingan teknis serta pendampingan teknologi secara langsung pada mitra sasaran. Kegiatan ini melibatkan mahasiswa Fakultas Pertanian dalam kegiatan demo teknologi pembuatan pupuk organik plus untuk media pembibitan dan penanaman tanaman sayuran-buah organik. Hasil kegiatan PKMI menunjukkan bahwa masyarakat sasaran dan masyarakat sekitar di lokasi pengabdian sangat antusias dan aktif berpartisipasi dalam kegiatan penyuluhan dan pendampingan budidaya tanaman hortikultura yang diberikan. Peserta target dan masyarakat setempat mengharapkan kesediaan tim pengabdian masyarakat untuk memberikan bimbingan teknis lanjutan teknologi pengembangan tanaman hortikultura secara organik yang memiliki nilai ekonomis lebih tinggi untuk memenuhi kebutuhan masyarakat secara umum.
PKM - Kelompok Tani Hortikultura Bawang Merah Pada Lahan Marginal Melalui Penerapan Teknologi Tepat Guna Ramah Lingkungan
Authors: Fyka Samsul Alam , Limi Muhammad Aswar , Awaluddin Andi
Published: March 2022

Desa Marga Cinta adalah salah satu desa di Kecamatan Moramo yang menjadi salah satu desa yang menjadi sumber pangan dari sektor hortikultura. Jenis hortikultura yang cukup menjanjikan untuk dikembangkan yaitu bawang merah. Salah satu mitra yang mengalami permasalahan dalam pengembangan usaha bawang merah yaitu Kelompok Tani Horti Utama. Permasalahan utama mitra adalah lahan marginal yang dimiliki untuk budidaya bawang merah mengalami penurunan kualitas lahan, akibat dari penggunaan pupuk kimia secara berlebihan. Adapun tujuan dari PKM ini adalah untuk meningkatkan pengetahuan kelompok tani Horti Utama tentang manfaat dari penggunaan teknologi tepat guna berbasis pertanian organik dan juga untuk meningkatkan keterampilan kelompok tani horti utama dalam membuat secara mandiri produk teknologi tepat guna berbasis sumberdaya lokal sehingga dapat digunakan pada lahan marginal untuk budidaya bawang merah. Kegiatan ini dilaksanakan sepanjang tahun 2021. Dengan metode kegiatan PKM terdiri dari 3 tahapan yaitu sosialisasi program, penyuluhan dan pelatihan pembuatan teknologi tepat guna serta pendampingan dan evaluasi. Hasil kegiatan PKM ini adalah dapat meningkatkan pemahaman mitra tentang manfaat dari pertanian organik melalui penerapan teknologi tepat guna (TTG) sebesar 60% dan juga peningkatkan pemahaman dan keterampilan kelompok tani tentang pembuatan teknologi tepat guna berbasis sumberdaya lokal meningkat sebesar 68%. Hal ini menunjukkan bahwa kelompok mitra mendapatkan manfaat terhadap program PKM yang dilaksanakan.
Upaya Menyiapkan Kehidupan Normal Baru Menghadapi Pandemi Covid-19 Melalui Edukasi Kesehatan Diri Dan Lingkungan Pada Masyarakat Di Desa Lombuea Kecamatan Moramo Utara Kabupaten Konawe Selatan
Authors: Cahyono Edi , Arman Arman , Nusantara Ambo Wonua , Sulastrianah Sulastrianah , Ransi Natalis
Published: March 2022

Desa Lombuea merupakan salah satu desa yang terletak di Kecamatan Moramo Utara Kabupaten Konawe Selatan Propinsi Sulawesi Tenggara. Untuk meningkatkan mutu kehidupan masyarakat, pemerintah telah menempuh berbagai upaya antara lain peningkatan pendidikan dan pembinaan serta penyuluhan kesehatan. Selain itu juga melakukan pembinaan usaha ekonomi yang meliputi pembinaan usaha produksi, pengolahan hasil pertanian serta pembinaan pemasaran dan kelembagaan ekonomi desa. Tujuan dari pengabdian kepada masyarakat terintegrasi KKN Tematik ini meliputi: 1) Memberikan wawasan kepada masyarakat akan pentingnya kesehatan keluarga untuk menyiapkan kehidupan normal baru dalam menghadapi penyebaran COVID-19 dan juga penyakit lainnya, 2) Memberikan pengetahuan pola hidup bersih dan sehat di lingkungan keluarga. 3) Meningkatnya pengetahuan akan pentingnya kesehatan gigi dalam kaitannya dengan jantung, 4) Meningkatnya pengetahuan masyarakat tentang pentingnya kesehatan ternak untuk menunjang produksi protein keluarga. Untuk mencapai sasaran dan tujuan yang telah digariskan dalam kegiatan pengabdian ini, maka metode yang digunakan adalah sosialisasi, penyuluhan dan pembimbingan secara langsungs hingga melalui kegiatan-kegiatan tersebut diharapkan dapat menciptakan masyarakat yang mandiri dan sehat. Secara umum tujuan utama dalam kegiatan pengabdian kepada masayarakat ini adalah untuk membangun masyarakat yang sehat dan mandiri. Untuk itu yang menjadi target kegiatan KKN-Tematik di Desa Lombuea Kecamatan Moramo Utara Kabupaten Konawe Selatan adalah: 1) Meningkatnya kesadaran masyarakat dalam prilaku hidup bersih dan sehat, 2) Meningkatnya pemahaman masyarakat terhadap kesehatan keluarga, 3) Meningkatnya pengetahuan masyarakat akan pentingnya pengelolaan lingkungan yang sehat, 4) Meningkatnya pengetahuan akan pentingnya kesehatan gigi dalam kaitannya dengan jantung, 6) Meningkatnya pengetahuan masyarakat akan pentingnya pemenuhan kebutuhan protein hewani dalam keluarga melalui edukasi kesehatan hewan ternak peliharaan.
Pemanfaatan Sistem Informasi Akademik dan Media Video Pembelajaran Sebagai Penunjang Kinerja Guru
Authors: Belluano Poetri Lestari Lokapitasari , Faradibah Amalia , Saiman La , AR Fatimah , Kasmira Kasmira
Published: March 2022

Academic Information System (xSIA) is a system that has one function to manage student data so as to provide convenience for teachers as the main users in recording the final grades of student learning outcomes each semester. The PKM program for teacher partners also provides technical training using the Snagit application to find out learning video material as an alternative method so that it can support teacher performance in the online teaching process. So that the Learning From Home (LFH) or Distance Learning Method can be implemented by school teachers at SDN No.133 Pari'risi Takalar. The training model applied uses the Preceptorship and Participatory training model. while the design phase of the application model used by Prototyping to graphically represent the workflow of the system. The objectives of this research are: 1) The existence of a web-based academic information system application, namely xSIA to report student assessments and application video tutorials. 2) Output in the form of the ILKOM Scientific Journal for the 2021 period UMI Computer Architecture or other Accredited journals. And 3) enrichment of teaching materials in Software Engineering courses.
RELASI QUADRUPLE HELIX DALAM PENGEMBANGAN ORGANISASI MASYARAKAT SIPIL PADA BADAN KESATUAN BANGSA DAN POLITIK KABUPATEN KOLAKA
Authors: Langka Meity , Suaib Eka , Alam Syamsul
Published: March 2022

Penelitian ini bertujuan untuk mendeskripsikan dan menganalisis relasi Quadruple Helix dalam pengembangan organisasi masyarakat sipil di Badan Kesatuan Bangsa dan Politik Kabupaten Kolaka . Peneltian ini menggunakan pendekatan kualitatif dengan informan yang berjumlah sebanyak 9 orang yang dipilih secara purposive sampling. Penelitian ini berfokus pada model Quadruple Helix dalam pengembangan organisasi masyarakat sipil. Pengumpualn data dalam penelitian ini melalui wawancara mendalam dan dokumentasi. Data yang diperoleh tersebut dianalisis secara kualitaif melalui analisis datan interaktif yang terdiri dari reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa relasi Quadruple Helix dalam pengembangan organisasi masyarakat sipil pada Badan Kesatuan Bangsa dan Politik Kabupaten Kolaka yaitu Pemerintah berperan sebagai regulator sekaligus berperan sebagai kontroler yang memiliki  peraturan dan tanggung jawab dalam mengembangkan objek., industri berperan sebagai  enabler yang menghadirkan infrastruktur, dengan mendukung perubahan pada  sumber daya manusia bussines dalam kolaboratif governance. Akademisi pada model Quadruple Helix berperan sebagai konseptor. Seperti melakukan standarisasi proses pada kegiatan yang dilakukan serta sertifikasi dan ketrampilan sumber daya manusia dan Media pada model Quadruple Helix berperan sebagai expender, dalam hal ini media berperan dalam mendukung publikasi dalam promosi dan membuat brand image. dalam program pengembangan kerjasama tersebut.Kata Kunci: Relasi, Quadruple Helix , Pengembangan Organisasi Masyarakat Sipil
IMPLEMENTASI KEBIJAKAN SISTEM KEUANGAN DESA DALAM PENGELOLAAN KEUANGAN DESA (STUDI KASUS DI KECAMATAN TONGAUNA KABUPATEN KONAWE)
Authors: Sarwono Edy , Banga Wempy , Larisu Zulfiah
Published: March 2022

Penelitian ini bertujuan untuk mendeskripsikan dan menganalisis implementasi kebijakan Aplikasi Sistem Keuangan Desa di Kecamatan Tongauna Kabupaten Konawe. Penelitian ini menggunakan pendekatan kualitatif dengan informan sebanyak 10 orang yang pilih secara purposive sampling. Data dalam penelitian ini diperoleh melalui wawancara, observasi dan studi pustaka. Data yang diperoleh tersebut kemudian dianalisis dalam penelitian ini terdiri dari pengamatan deskriptif, analisis domein, pengamatan terfokus dan analisis tema. Hasil penelitian ini menunjukkan bahwa implementasi Sistem Keuangan Desa dalam pengelolaan keuangan desa di Kecamatan Tongauna Kabupaten Konawe meliputi penerapan separangkat aturan, sumber daya untuk menggerakkan kegiatan (sarana dan parasana, sumberdaya keuangan, dan pelaksana (implementor) dan manfaat bagi masyarakat. Dimana sumberdaya menjadi salah satu kendala terbesar dalam Implementasi Siskeudes dalam pengelolaan keuangan desa di Kecamatan Tongauna Kabupaten Konawe.Kata Kunci: Implementasi, kebijakan, Sistem Keuangan Desa
A Realistic Analysis of the Stuxnet Cyber-attack
Authors: Mohee Ahmad
Published: March 2022

This paper presented a realistic analysis of Stuxnet cyber-attack, aiming to uncover the complexities associated with the launch decision-making process. It managed to answer the question: Why were Stuxnet cyber-attack adopted, as a strategic choice for managing the conflict instead of traditional choices? It also gave an understanding for the security challenges and the strategic implications that cyber-attack had to pose. Finally, it reached a number of conclusions to the effect that: Realism, as a theory mostly concerned with issues of national security and power, can be a very suitable theoretical framework for analyzing and understanding cyber-attacks. The analysis confirmed that realism is an appropriate framework for identifying important issues related to security in the cyberspace and can provide useful insights into some of the enduring characteristics of international relations in the cyberspace.
Editorial
Authors:   College of Education, University of Otago, Dunedin, New Zealand, Sexton Steven S.
Published: March 2022

Editorial Volume 33, Issue 1
Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa
Authors: Jabłońska Joanna , Dubrowska Kamila , Augustyniak Adrian , Kordas Marian , Rakoczy Rafał
Published: March 2022

Pseudomonas aeruginosa is a producer of desired secondary metabolites, including pyocyanin. Potential uses of this pigment urge a search for improved production methods. Recent trends in bioprocessing show the potential of the use of electromagnetic fields (EMFs) to influence the growth of microorganisms and even modulate the concentration of bioproducts. Here, we aimed at assessing the influence of rotating magnetic field (RMF) and static magnetic field (SMF) on pyocyanin production, growth rate, and respiration of P. aeruginosa. Moreover, exposure time to EMFs (2, 6, and 12 h) and culture volume (10 and 50 ml) were initially assessed. P. aeruginosa was cultivated in magnetically assisted reactors with 5 and 50 Hz RMF (magnetic induction of 24.32 and 42.64 mT, respectively) and SMF (−17.37 mT). Growth kinetics was assessed with Gompertz equation. The viability was tested using resazurin assay, whereas pyocyanin production by chloroform-HCl methodology. The growth of P. aeruginosa was slightly stimulated by exposure to a RMF with 50 Hz (108% related to the control) and significantly by SMF (132% related to the control), while RMF 5 Hz exposure prolonged the time of inflection (in comparison to RMF 50 Hz and SMF). The 6-h exposure to EMFs resulted in the highest pyocyanin production in comparison to the control, indicating a relationship between exposure time and product concentration. Moreover, cultures led in smaller volumes produced more pyocyanin. Our findings show that the use of different EMF types, frequency, and exposition time and volume could be used interchangeably to obtain different bioprocess aims.
A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576
Authors: Liu Jun , Zhang Xinyu , Liu Guodong , Zhao Guoqin , Fang Xiaoran , Song Xin
Published: March 2022

Sophorolipids (SLs), an important biosurfactant produced by S. bombicola, were one of the most potential substitutes for chemical surfactants. Few reports on the transcriptional regulation of SLs synthesis and the engineered strains with high-yield SLs were available. In this study, a Rim9-like protein (Rlp) and three transcription factors (ztf1, leu3, gcl) were mined and analyzed, and a progressive enhancement of SLs production was achieved through cumulative knockouts of three genes. The sophorolipid production of ΔrlpΔleu3Δztf1 reached 97.44 g/L, increased by 50.51% than that of the wild-type strain. Compared with the wild-type strain, the flow of glucose to SLs synthesis pathways was increased, and the synthesis of branched-chain amino acids was reduced in ΔrlpΔleu3Δztf1. The amount of UDP-glucose, the substrate for two glycosyltransferases, also increased, and the expression level of the key genes sble and UGPase for SLs synthesis increased by 2.2 times, respectively. The multiple-gene knockout strategy was proved to be highly effective to construct the engineered strain with high-yield SLs production, and this strain was a superior strain for industrial fermentation of SLs and reduced SLs production costs.
Synthesis of Fe3O4/PDA Nanocomposites for Osteosarcoma Magnetic Resonance Imaging and Photothermal Therapy
Authors: Zhang Yifei , Ning Rende , Wang Wei , Zhou Yejin , Chen Yao
Published: March 2022

Osteosarcomas commonly develop in the metaphysis of the long diaphysis, resulting in pronounced malignancy and high rates of early pulmonary metastasis. At present, osteosarcoma patients exhibit relatively poor survival rates owing these metastases and to the emergence of tumor chemoresistance. As such, there is an urgent need to identify other approaches to treating affected patients. Herein, we synthesized Fe3O4@PDA nanocomposites that exhibited excellent biocompatibility and low toxicity in human and animal model systems. The resultant nanoparticles were able to improve T2 magnetic resonance imaging and to enhance the signal-to-noise ratio associated with osteosarcoma tumors in animal models. Moreover, we were able to successfully leverage these Fe3O4@PDA particles as a photothermal agent capable of significantly inhibiting the growth of tumors and preventing their metastasis to the lung compartment. Together, these results highlight a novel therapeutic platform that has the potential to guide both the more effective diagnosis and treatment of osteosarcoma patients in clinical applications.
Reality Model-Based Facility Management Framework for Existing Building
Authors: Duong Hung Duy , Lin Jacob J.
Published: March 2022

Facility management (FM) is the most extended phase of the entire life cycle of buildings and incurs the highest expenses. However, limited quality drawings and historical data make information flow more complicated for many existing buildings and lead to inefficient FM. This research proposes a systematic and comprehensive reality point cloud model-based framework to support the FM process with feasible visual data collection. Specifically, this framework covers an end-to-end workflow of using a reality model to implement FM strategies, including visual data capture process for generating a reality model; FM information attachment in a reality model for issue queries; and information flow for issue tracking. The resulting reality model captures with critical and necessary FM data input can be used as an asset information model (AIM). We have also defined stakeholders' responsibilities in implementing FM and how the data are transferred, updated, and used within a reality point cloud-based AIM by stakeholders. The framework is evaluated by interviewing FM experts, and the results demonstrate that implementing the framework could improve the FM process's efficiency by using reality models.
Isolevuglandins Scavenger Ameliorates Myocardial Ischemic Injury by Suppressing Oxidative Stress, Apoptosis, and Inflammation
Authors: Guo Junjie , Xu Fengqiang , Ji Hongwei , Jing Yajun , Shen Li , Weng Xinyu , Hu Longgang
Published: March 2022

Augmented levels of reactive isolevuglandins (IsoLGs) are responsible for cardiovascular diseases. The role of IsoLGs in myocardial infarction (MI) remains elusive. Here we explored the effect of IsoLGs scavenger 2-hydroxybenzylamine (2-HOBA) in post-infarction cardiac repair. We observed that infarcted cardiac tissues expressed high IsoLGs in mice. Following MI injury, 2-HOBA treated mice displayed decreased infarction area and improved heart function compared with the saline-treated group. Moreover, 2-HOBA effectively attenuated MI-induced cardiac remodeling, oxidative stress, apoptosis, and inflammation. 4-hydroxybenzylamine (4-HOBA), a less reactive isomer of 2-HOBA, barely antagonized the MI-induced injury. These findings suggest that IsoLGs elimination may be helpful in MI therapy.
The Effect of Silicate Ions on the Separation of Lithium From Geothermal Fluid
Authors: Lee Junbeum , Chung Eunhyea
Published: March 2022

In an enhanced geothermal system (EGS), geothermal energy in rocks with insufficient permeability or fluid saturation can be used by creating artificial geothermal reservoirs. Generally, EGS geothermal fluid contains high concentrations of total dissolved solids that originated from various geochemical reactions between the fluid in the reservoir and the minerals in the rock. For example, the concentration of lithium ions are measured approximately 150 mg/L, and several researchers have focused on the recovery of lithium in the geothermal fluid using various methods, one of which is liquid extraction. Solvent extraction has been used to recover lithium from various sources, and successful recovery efficiency have been attained. However, the geothermal fluid in EGS reservoirs contains high concentrations of SiO2, which might inhibit the selective recovery of lithium. Thus, in this study, two consecutive stages of solvent extraction were used to separate the lithium from the geothermal fluid that contained different concentrations of SiO2 ions. The divalent ions were removed in the first stage, and the lithium ions were extracted effectively in the second stage. The SiO2 inhibits the selective recovery of lithium in the first stage to a greater extent than it does in the second stage. The spectroscopy data shows a decrease of the organic solvents main functional group (P=O & P-O-H) absorbance that reacts with the metal ions of the geothermal water after extraction however the intensity difference was reduced as the SiO2 concentrations increases. Silicate ions can be problematic due to the formation of scaling in EGSs, so controlling its concentration in the geothermal reservoir would be beneficial for the long-term operation of EGSs and for the successful recovery of valuable metal resources from EGS reservoirs.
Evolution of E. coli Phytase Toward Improved Hydrolysis of Inositol Tetraphosphate
Authors: Herrmann Kevin R. , Brethauer Christin , Siedhoff Niklas E. , Hofmann Isabell , Eyll Johanna , Davari Mehdi D. , Schwaneberg Ulrich , Ruff Anna Joëlle
Published: March 2022

Protein engineering campaigns are driven by the demand for superior enzyme performance under non-natural process conditions, such as elevated temperature or non-neutral pH, to achieve utmost efficiency and conserve limited resources. Phytases are industrial relevant feed enzymes that contribute to the overall phosphorus (P) management by catalyzing the stepwise phosphate hydrolysis from phytate, which is the main phosphorus storage in plants. Phosphorus is referred to as a critical disappearing nutrient, emphasizing the urgent need to implement strategies for a sustainable circular use and recovery of P from renewable resources. Engineered phytases already contribute today to an efficient phosphorus mobilization in the feeding industry and might pave the way to a circular P-bioeconomy. To date, a bottleneck in its application is the drastically reduced hydrolysis on lower phosphorylated reaction intermediates (lower inositol phosphates, ≤InsP4) and their subsequent accumulation. Here, we report the first KnowVolution campaign of the E. coli phytase toward improved hydrolysis on InsP4 and InsP3. As a prerequisite prior to evolution, a suitable screening setup was established and three isomers Ins(2,4,5)P3, Ins(2,3,4,5)P4 and Ins(1,2,5,6)P4 were generated through enzymatic hydrolysis of InsP6 and subsequent purification by HPLC. Screening of epPCR libraries identified clones with improved hydrolysis on Ins(1,2,5,6)P4 carrying substitutions involved in substrate binding and orientation. Saturation of seven positions and screening of, in total, 10,000 clones generated a dataset of 46 variants on their activity on all three isomers. This dataset was used for training, testing, and inferring models for machine learning guided recombination. The PyPEF method used allowed the prediction of recombinants from the identified substitutions, which were analyzed by reverse engineering to gain molecular understanding. Six variants with improved InsP4 hydrolysis of >2.5 were identified, of which variant T23L/K24S had a 3.7-fold improved relative activity on Ins(2,3,4,5)P4 and concomitantly shows a 2.7-fold improved hydrolysis of Ins(2,4,5)P3. Reported substitutions are the first published Ec phy variants with improved hydrolysis on InsP4 and InsP3.
Guiding Principles for the Clinical Use and Selection of Microcatheters in Complex Coronary Interventions
Authors: Goel Pravin K. , Sahu Ankit Kumar , Kasturi Sridhar , Roy Sanjeeb , Shah Nimit , Parikh Prakashvir , Chadha Davinder S.
Published: March 2022

The use of microcatheters as a coronary interventional tool for a therapeutic approach to complex coronary interventions like bifurcation lesions, ostial location, tortuous anatomy, angled takeoffs, coronary calcification, and chronic total occlusion (CTO) percutaneous coronary intervention (PCI) is growing among cardiologists across the country. During the treatment of such complex lesions, microcatheters play an essential part of the tool kit with both single-lumen and double-lumen microcatheters (DLMs) having their specific niche areas. The selection of microcatheters involves a detailed understanding of the microcatheter specification, lesion anatomy, lesion location, vessel tortuosity and trajectory, and crossing techniques. The selection of appropriate crossing techniques with different microcatheters increases success rates of PCI, reduces procedural time and contrast use, and lowers the radiation. However, the use of microcatheters and their technicalities have not yet fully realized by many operators and their true scope has not been fully explored. This article discusses and summarizes the thoughts and key opinions of experts in this field.
Aortic Valve Leaflet Shape Synthesis With Geometric Prior From Surrounding Tissue
Authors: Hagenah Jannis , Scharfschwerdt Michael , Ernst Floris
Published: March 2022

Even though the field of medical imaging advances, there are structures in the human body that are barely assessible with classical image acquisition modalities. One example are the three leaflets of the aortic valve due to their thin structure and high movement. However, with an increasing accuracy of biomechanical simulation, for example of the heart function, and extense computing capabilities available, concise knowledge of the individual morphology of these structures could have a high impact on personalized therapy and intervention planning as well as on clinical research. Thus, there is a high demand to estimate the individual shape of inassessible structures given only information on the geometry of the surrounding tissue. This leads to a domain adaptation problem, where the domain gap could be very large while typically only small datasets are available. Hence, classical approaches for domain adaptation are not capable of providing sufficient predictions. In this work, we present a new framework for bridging this domain gap in the scope of estimating anatomical shapes based on the surrounding tissue's morphology. Thus, we propose deep representation learning to not map from one image to another but to predict a latent shape representation. We formalize this framework and present two different approaches to solve the given problem. Furthermore, we perform a proof-of-concept study for estimating the individual shape of the aortic valve leaflets based on a volumetric ultrasound image of the aortic root. Therefore, we collect anex-vivoporcine data set consisting of both, ultrasound volume images as well as high-resolution leaflet images, evaluate both approaches on it and perform an analysis of the model's hyperparameters. Our results show that using deep representation learning and domain mapping between the identified latent spaces, a robust prediction of the unknown leaflet shape only based on surrounding tissue information is possible, even in limited data scenarios. The concept can be applied to a wide range of modeling tasks, not only in the scope of heart modeling but also for all kinds of inassessible structures within the human body.
The Influence of the Tibetan Plateau Monsoon on Summer Precipitation in Central Asia
Authors: Zhang Shaobo , Meng Lixia , Zhao Yong , Yang Xianyu , Huang Anning
Published: March 2022

As a unique climate phenomenon induced by the Tibetan Plateau (TP) heat flux, the TP monsoon is closely connected with the global climate, especially pertaining to the TP. However, the current research focuses more on the influence of the TP monsoon on the TP and eastern China, but not including Central Asia. This paper analyzed the relationship between the TP monsoon index and Central Asia summer precipitation by JRA55 reanalysis data and Global Precipitation Climatology Centre (GPCC) monthly precipitation. The results showed a significant positive relationship between the TP monsoon index and summer precipitation in Central Asia. When the TP monsoon was strong, there was cold advection in the upper troposphere over Central Asia, and the resulting thermal wind caused a cyclonic circulation anomaly in the mid-upper troposphere over Central Asia. This in turn led to a cyclonic circulation anomaly to water vapor transport in the lower troposphere. The abnormal upward movement also caused more precipitation in this area, which explains the positive correlation between the TP monsoon and the precipitation in Central Asia. Based on this physical mechanism, the temperature of the mid-upper troposphere over Central Asia was closely related to the TP monsoon, and it was a key factor that affected summer precipitation changes in Central Asia.
Adaptive Interpolation Method for Generalized Triangular Prism (GTP) Geological Model Based on the Geometric Smoothness Rule
Authors: Sun Liming , Wei Yingqi , Cai Hong , Xiao Jianzhang , Yan Jun , Wu Shuaifeng
Published: March 2022

Three dimensional (3D) geological model is frequently used to represent the geological conditions of the subsurface. The generalized triangular prism (GTP) model designed for borehole sampling data is a spatial data model that could retain the internal connection between the three adjacent boreholes and distinguish between the bedding and cross-bedding directions, which is proper for accurate 3D geological modeling. The traditional building method cannot consider two factors: the borehole distance is usually longer than the stratigraphic thickness, and the top and the bottom surface have different accuracy at the same time. In this study, we describe the new interpolation method for the GTP 3D geological model to improve the model accuracy with sparse borehole data. Firstly, definition and calculation method of the GTP model smoothness are proposed to measure the model smoothness and accuracy degree, which are used to decide whether the GTP voxel requires interpolation. Secondly, the virtual borehole design and calculation method for the GTP voxel subdivision in terms of the GTP geometric smoothness are discussed in detail. Finally, the GTP adaptive interpolation can be performed through the GTP voxel subdivision and the geometric optimization rebuilding. This method could adaptively interpolate the existing GTP model by local updating without changing the GTP model structure, it has high efficiency compared to the classical method. In addition, the feasibility and accuracy of this method could be proven by the actual case. The study will provide a new and reliable interpolation method for the GTP model, and it is also conducive to economic geology related research.
Molecular Dynamics Simulation of the Nanoindentation of Coal Vitrinite
Authors: Liu Hewu , Hou Chenliang , Song Yu
Published: March 2022

Coal deformation is closely correlated with the distribution of organic maceral groups, however, molecular dynamics (MD) simulations of vitrinite nanoindentation have rarely been conducted. In this study, the vitrinite substrate for indentation was constructed utilizing polymer consistent force field (PCFF), and a spherical ghost indenter was used for loading. The results showed that: 1) In the indentation process, some of the vitrinite atoms overcame the energy barrier to move, with the most important deformation mechanism including the sliding, bending, and reorientation of vitrinite molecular chains, leading to the formation of a shearing transformation zone (STZ), which was also found to contain structural defects and stacking of aromatic structures. 2) The distribution of atomic displacements in the vitrinite substrate could be subdivided into distinct regions, with slippage at the region boundaries producing shear bands. 3) The surface morphology and mechanical properties obtained from the nanoindentation simulation were similar to experimental results from the literature, indicating that MD simulations are a powerful tool for studying coal nanoindentation. The results from this study increase the current scientific understanding of the mechanical properties of vitrinite by providing a new perspective that elucidates the nanoscale structural evolution occurring during the indentation process.
Case Report: Hepatic Adenomatosis in a Patient With Prader–Willi Syndrome
Authors: Dauleh Hajar , Soliman Ali , Haris Basma , Khalifa Amal , Al Khori Noor , Hussain Khalid
Published: March 2022

Prader–Willi syndrome (PWS) is a genetic disorder caused by the lack of expression of genes on the paternally inherited chromosome region 15q11.2-q13. It is a multisystem disorder that is characterized by severe hypotonia with poor suck and feeding difficulties in early infancy, followed in early childhood by excessive eating and gradual development of morbid obesity. The incidence of type 2 diabetes mellitus is high, particularly in obese patients. Non-alcoholic fatty liver disease has also been reported in some patients with PWS. Liver adenomatosis is a benign vascular lesion of the liver, defined by the presence of >10 adenomas, in the otherwise healthy liver parenchyma. We report the first case of a patient with PWS with severe obesity, type 2 diabetes mellitus, and non-alcoholic fatty liver who also developed liver adenomatosis, review the pediatric literature on liver adenomatosis, and discuss the potential underlying mechanisms.
Radioactive Iodine for the Treatment of Subclinical Thyrotoxicosis Grade 1 and 2: Outcome of up to 18-Year Follow Up
Authors: Phowira Jason , Coffey Katherine L. , Bartholomew Peter H. , Vennart Nicholas , Moreira Matheus , Emerson Hannah , Kennedy David , Weaver Jolanta U.
Published: March 2022

Background

Subclinical thyrotoxicosis (SCT) is associated with significant morbidity and mortality, specifically increased risk of atrial fibrillation and cardiovascular death. The management is ill-defined due to the scarcity of randomised controlled studies. Some clinicians recommend radioiodine (RAI) treatment however its long-term outcome is unknown. Therefore, further data is needed to provide robust evidence-based guidelines.

Methods

A prospective, single-protocol analysis of the outcome of SCT patients (Grade 1; 0.1-0.4 mIU/L and Grade 2; <0.1 mIU/L) treated with mean dose of 427 MBq of I131, followed up for up to 18 years. Thyroid function tests were measured at 4-6 weeks, 3-, 6-, and 12-months post-RAI, and annually thereafter. Cure was defined as achieving a euthyroid/hypothyroid state.

Results

Seventy-eight patients with a median age of 68 years (range 36-84) and varying aetiology [55 toxic multinodular goitre (TMNG), 10 toxic nodule (TN) and 13 Graves’ disease (GD)] were followed up for a median period of 7.5 years (range 1-18). The cure rate was 100%. The rates of hypothyroidism in TMNG, TN and GD were 23.6%, 30% and 38.5% respectively. The median time to hypothyroidism was 6 and 12 months in GD and TMNG/TN respectively. No differences in outcome between Grade 1 versus Grade 2 were observed.

Conclusion

RAI using single mean dose of 427 MBq is effective and safe, irrespective of aetiology or grade of TSH suppression. GD patients become hypothyroid within the first year, whilst TMNG/TN for up to 9-years. Thus after 12 months of follow up, annual thyroid function monitoring is advised.
Elevated Serum Leptin Levels as a Predictive Marker for Polycystic Ovary Syndrome
Authors: Peng Yuanyuan , Yang Hongyue , Song Jiahui , Feng Di , Na Zhijing , Jiang Hongyu , Meng Yaxin , Shi Bei , Li Da
Published: March 2022

Background

Leptin may have important implications in polycystic ovary syndrome (PCOS)-related metabolic disorders. However, the changes in serum leptin levels in patients with PCOS and its predictive value for PCOS remain obscure. We intend to analyze the association between leptin and PCOS in this study.

Materials and Methods

The study comprised 89 patients with PCOS and 139 individuals without PCOS. Each group was stratified as either normal- or hyper-fasting serum insulin (FSI), and lean or overweight/obese; and the patients were further categorized as normal- or hyper-androgenic. The validity of leptin toward the diagnosis of PCOS, or leptin combined with total testosterone, dehydroepiandrosterone sulfate (DHEAS), and free testosterone was estimated by receiver operating characteristic (ROC) curves, and correlations between paired variables was estimated by Spearman’s rank correlation coefficient. Associations between the clinical and metabolic variables and PCOS were analyzed via logistic regression.

Results

The serum leptin levels of patients with PCOS were significantly higher than that of the control, and especially the PCOS in hyper-FSI, hyperandrogenimic and overweight/obese subgroups. The area under the ROC curve (AUC) of leptin was 74%, with cutoff value, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) 11.58 ng/mL, 77.5%, 62.6%, 57.0%, and 81.3%, respectively. Combined leptin and anti-Müllerian hormone (AMH) had the highest AUC (92.3%), excellent sensitivity (93.3%), moderate specificity (78.3%), PPV (73.5%) and NPV (94.8%). Serum leptin levels of the patients were correlated with the FSI, fasting plasma glucose (FPG), homeostasis model assessment of insulin resistance (HOMA-IR), body mass index (BMI), and total testosterone levels. Elevated serum leptin was associated with a high risk of PCOS [P = 0.015; OR (95% CI) 1.128 (1.024–1.244)].

Conclusion

Substantially elevated serum leptin is significantly associated with PCOS. These findings warrant further investigations into the function of leptin in the pathogenesis of PCOS.
Diabetes Mellitus Predicts Weight Gain After Surgery in Patients With Acromegaly
Authors: Jang Han Na , Kim Yong Hwy , Kim Jung Hee
Published: March 2022

Objective

Metabolic complications are common in patients with acromegaly. However, this occasionally does not improve post-surgery and may be related to postoperative weight gain. We aimed to investigate the postoperative weight change and factors associated with postoperative weight gain in patients with acromegaly.

Design and Methods

Overall, 113 consecutive patients with body weight records pre- and 3–6 months post-surgery between October 2009 and March 2021 were enrolled. Patients were divided into three groups: weight loss (weight decrease ≥3%), stable, and weight gain (weight increase ≥3%). Hormone status, metabolic comorbidities, and anthropometric parameters were compared between the groups.

Results

Among 113 patients, 29 (25.7%) and 26 (23.0%) patients lost and gained weight, respectively, post-surgery. There were no significant differences in baseline characteristics, including age at diagnosis, sex, body mass index, and growth hormone levels among the three groups. The prevalence of diabetes mellitus at diagnosis was significantly higher in the weight gain group than in the other groups. Patients with diabetes (n=22) had a 5.2-fold higher risk of postoperative weight gain than those with normal glucose tolerance (n=37) (P=0.006). In the diabetes mellitus group, the percentage lean mass decreased (-4.5 [-6.6–2.0]%, P=0.002), and the percentage fat mass significantly increased post-surgery (18.0 [4.6–36.6]%, P=0.003), whereas the normal glucose tolerance group did not show body composition changes post-surgery.

Conclusion

In patients with acromegaly, 23% experienced ≥3% weight gain post-surgery. Diabetes mellitus at diagnosis is a significant predictor of weight and fat gain post-surgery.