bluuebunny/crossref_metadata_2025 huggingface dataset:
The Gut Microbiome and Gastrointestinal Toxicities in Pelvic Radiation Therapy: A Clinical Review
Authors: Oh Byeongsang , Eade Thomas , Lamoury Gillian , Carroll Susan , Morgia Marita , Kneebone Andrew , Hruby George , Stevens Mark , Boyle Frances , Clarke Stephen , Corless Brian , Molloy Mark , Rosenthal David , Back Michael
Published: May 2021

Background: Gastrointestinal (GI) toxicities are common adverse effects of pelvic radiotherapy (RT). Several recent studies revealed that toxicity of RT is associated with dysbiosis of the gut microbiome. Method: A literature search was conducted in electronic databases Medline, PubMed, and ScienceDirect, with search terms “microbiome and/or microbiota” and “radiotherapy (RT) and/or chemoradiation therapy (CRT)” and “cancer”, and the relevant literature were selected for use in this article. Results: Eight prospective cohort studies were selected for review with a total of 311 participants with a range of 15–134 participants within these studies. The selected studies were conducted in patients with gynaecological (n = 3), rectal (n = 2), or prostate cancers (n = 1), or patients with various types of malignancies (n = 2). Three studies reported that cancer patients had significantly lower alpha diversity compared with healthy controls. Seven studies found that lower alpha diversity and modulated gut microbiome were associated with GI toxicities during and after pelvic RT (n = 5) and CRT (n = 2), whereas one study found that beta diversity was related to a complete response following CRT. Two further studies reported that fatigue was associated with dysbiosis of the gut microbiome and low alpha diversity during and after RT, and with dysbiosis of the gut microbiome and diarrhoea, respectively. Conclusion: Gut microbiome profiles are associated with GI toxicities and have the potential to predict RT/CRT-induced toxicities and quality of life (QoL) in patients undergoing those treatments. Further robust randomized controlled trials (RCTs) are required to elucidate the effect of gut microbiome profiles on RT-related adverse effects and responses to RT.
Toward a Personalized Therapy in Soft-Tissue Sarcomas: State of the Art and Future Directions
Authors: Montella Liliana , Altucci Lucia , Sarno Federica , Buonerba Carlo , De Simone Stefano , Facchini Bianca Arianna , Franzese Elisena , De Vita Ferdinando , Tafuto Salvatore , Berretta Massimiliano , Facchini Gaetano
Published: May 2021

Soft-tissue sarcomas are rare tumors characterized by pathogenetic, morphological, and clinical intrinsic variability. Median survival of patients with advanced tumors are usually chemo- and radio-resistant, and standard treatments yield low response rates and poor survival results. The identification of defined genomic alterations in sarcoma could represent the premise for targeted treatments. Summarizing, soft-tissue sarcomas can be differentiated into histotypes with reciprocal chromosomal translocations, with defined oncogenic mutations and complex karyotypes. If the latter are improbably approached with targeted treatments, many suggest that innovative therapies interfering with the identified fusion oncoproteins and altered pathways could be potentially resolutive. In most cases, the characteristic genetic signature is discouragingly defined as “undruggable”, which poses a challenge for the development of novel pharmacological approaches. In this review, a summary of genomic alterations recognized in most common soft-tissue sarcoma is reported together with current and future therapeutic opportunities.
Extracellular Acidification Induces Lysosomal Dysregulation
Authors: Ordway Bryce , Gillies Robert J. , Damaghi Mehdi
Published: May 2021

Many invasive cancers emerge through a years-long process of somatic evolution, characterized by an accumulation of heritable genetic and epigenetic changes and the emergence of increasingly aggressive clonal populations. In solid tumors, such as breast ductal carcinoma, the extracellular environment for cells within the nascent tumor is harsh and imposes different types of stress on cells, such as hypoxia, nutrient deprivation, and cytokine inflammation. Acidosis is a constant stressor of most cancer cells due to its production through fermentation of glucose to lactic acid in hypoxic or normoxic regions (Warburg effect). Over a short period of time, acid stress can have a profound effect on the function of lysosomes within the cells exposed to this environment, and after long term exposure, lysosomal function of the cancer cells can become completely dysregulated. Whether this dysregulation is due to an epigenetic change or evolutionary selection has yet to be determined, but understanding the mechanisms behind this dysregulation could identify therapeutic opportunities.
Neuroinflammation: Integrated Nervous Tissue Response through Intercellular Interactions at the “Whole System” Scale
Authors: Nosi Daniele , Lana Daniele , Giovannini Maria Grazia , Delfino Giovanni , Zecchi-Orlandini Sandra
Published: May 2021

Different cell populations in the nervous tissue establish numerous, heterotypic interactions and perform specific, frequently intersecting activities devoted to the maintenance of homeostasis. Microglia and astrocytes, respectively the immune and the “housekeeper” cells of nervous tissue, play a key role in neurodegenerative diseases. Alterations of tissue homeostasis trigger neuroinflammation, a collective dynamic response of glial cells. Reactive astrocytes and microglia express various functional phenotypes, ranging from anti-inflammatory to pro-inflammatory. Chronic neuroinflammation is characterized by a gradual shift of astroglial and microglial phenotypes from anti-inflammatory to pro-inflammatory, switching their activities from cytoprotective to cytotoxic. In this scenario, the different cell populations reciprocally modulate their phenotypes through intense, reverberating signaling. Current evidence suggests that heterotypic interactions are links in an intricate network of mutual influences and interdependencies connecting all cell types in the nervous system. In this view, activation, modulation, as well as outcomes of neuroinflammation, should be ascribed to the nervous tissue as a whole. While the need remains of identifying further links in this network, a step back to rethink our view of neuroinflammation in the light of the “whole system” scale, could help us to understand some of its most controversial and puzzling features.
Experimental Study of Plasma Plume Analysis of Long Pulse Laser Irradiates CFRP and GFRP Composite Materials
Authors: Ma Yao , Xin Chao , Zhang Wei , Jin Guangyong
Published: May 2021

The application of laser fabrication of fiber-reinforced polymer (FRP) has an irreplaceable advantage. However, the effect of the plasma generated in laser fabrication on the damage process is rarely mentioned. In order to further study the law and mechanism of laser processing, the laser process was measured. CFRP and GFRP materials were damaged by a 1064 nm millisecond pulsed laser. Moreover, the propagation velocity and breakdown time of plasma plume were compared. The results show that GFRP is more vulnerable to breakdown than CFRP under the same conditions. In addition, the variation of plasma plume and material surface temperature with the number of pulses was also studied. The results show that the variation trend is correlated, that is, the singularities occur at the second pulse. Based on the analysis of experimental phenomena, this paper provides guidance for plasma phenomena in laser processing of composite materials.
Re-Irradiation for Locally Recurrent Lung Cancer: A Single Center Retrospective Analysis
Authors: Grambozov Brane , Nussdorfer Evelyn , Kaiser Julia , Gerum Sabine , Fastner Gerd , Stana Markus , Gaisberger Christoph , Wass Romana , Studnicka Michael , Sedlmayer Felix , Zehentmayr Franz
Published: May 2021

The treatment of locally recurrent lung cancer is a major challenge for radiation-oncologists, especially with data on high-dose reirradiation being limited to small retrospective studies. The aim of the present study is to assess overall survival (OS) for patients with locally recurrent lung cancer after high-dose thoracic reirradiation. Thirty-nine patients who were re-irradiated for lung cancer relapse between October 2013 and February 2019 were eligible for the current retrospective analysis. All patients were re-irradiated with curative intent for in-field tumor recurrence. The diagnostic work-up included a mandatory 18F-FDG-PET-CT scan and—if possible—histological verification. The ECOG was ≤2, and the interval between initial and second radiation was at least nine months. Thirty patients (77%) had non-small cell lung cancer (NSCLC), eight (20%) had small cell lung cancer (SCLC), and in one patient (3%) histological confirmation could not be obtained. More than half of the patients (20/39, 51%) received re-treatment with dose differentiated accelerated re-irradiation (DART) at a median interval of 20.5 months (range: 6–145.3 months) after the initial radiation course. A cumulative EQD2 of 131 Gy (range: 77–211 Gy) in a median PTV of 46 mL (range: 4–541 mL) was delivered. Patients with SCLC had a 3 mL larger median re-irradiation volume (48 mL, range: 9–541) compared to NSCLC patients (45 mL, range: 4–239). The median cumulative EQD2 delivered in SCLC patients was 84 Gy (range: 77–193 Gy), while NSCLC patients received a median cumulative EQD2 of 135 Gy (range: 98–211 Gy). The median OS was 18.4 months (range: 0.6–64 months), with tumor volume being the only predictor (p < 0.000; HR 1.007; 95%-CI: 1.003–1.012). In terms of toxicity, 17.9% acute and 2.6% late side effects were observed, with a toxicity grade >3 occurring in only one patient. Thoracic high dose reirradiation plays a significant role in prolonging survival, especially in patients with small tumor volume at recurrence.
Burrowing Parrots Cyanoliseus patagonus as Long-Distance Seed Dispersers of Keystone Algarrobos, Genus Prosopis, in the Monte Desert
Authors: Blanco Guillermo , Romero-Vidal Pedro , Carrete Martina , Chamorro Daniel , Bravo Carolina , Hiraldo Fernando , Tella José L.
Published: May 2021

Understanding of ecosystem structure and functioning requires detailed knowledge about plant–animal interactions, especially when keystone species are involved. The recent consideration of parrots as legitimate seed dispersers has widened the range of mechanisms influencing the life cycle of many plant species. We examined the interactions between the burrowing parrot Cyanoliseus patagonus and two dominant algarrobo trees (Prosopis alba and Prosopis nigra) in the Monte Desert, Argentina. We recorded the abundance and foraging behaviour of parrots; quantified the handling, consumption, wasting, and dispersal of ripe and unripe pods; and tested the viability of soft and hard ripe seeds wasted and transported by parrots. We found a high abundance of burrowing parrots. They predated on soft seeds from unripe pods while exclusively feeding upon pulp wrapping hard seeds from ripe pods. Frequent pod wasting beneath the plant or transport at a distance invariably implied the dispersal of multiple seeds in each event. Moreover, soft seeds retained viability after desiccation outside the mother plant, suggesting effective seed dispersal after partial pod predation due to a predator satiation effect. In about half of the foraging flocks, at least one parrot departed in flight with pods in its beak, with 10–34% of the flock components moving pods at distances averaging 238 m (P. alba) and 418 m (P. nigra). A snapshot sampling of faeces from livestock and wild mammals suggested a low frequency of seed dispersal by endozoochory and secondary dispersal by ants and dung beetles. The nomadic movements and long flights of burrowing parrots between breeding and foraging sites can lead to the dispersal of huge amounts of seeds across large areas that are sequentially exploited. Further research should evaluate the role of the burrowing parrot as a functionally unique species in the structure of the Monte Desert woods and the genetic structure of algarrobo species.
Boreal Bird Ecology, Management and Conservation
Authors: McNulty Stacy , Glennon Michale , Foss Carol
Published: May 2021

The circumpolar boreal forest covers approximately 12,000,000 km2 and is one of the world’s most extensive biomes [...]
A Hybridization of Dragonfly Algorithm Optimization and Angle Modulation Mechanism for 0-1 Knapsack Problems
Authors: Wang Lin , Shi Ronghua , Dong Jian
Published: May 2021

The dragonfly algorithm (DA) is a new intelligent algorithm based on the theory of dragonfly foraging and evading predators. DA exhibits excellent performance in solving multimodal continuous functions and engineering problems. To make this algorithm work in the binary space, this paper introduces an angle modulation mechanism on DA (called AMDA) to generate bit strings, that is, to give alternative solutions to binary problems, and uses DA to optimize the coefficients of the trigonometric function. Further, to improve the algorithm stability and convergence speed, an improved AMDA, called IAMDA, is proposed by adding one more coefficient to adjust the vertical displacement of the cosine part of the original generating function. To test the performance of IAMDA and AMDA, 12 zero-one knapsack problems are considered along with 13 classic benchmark functions. Experimental results prove that IAMDA has a superior convergence speed and solution quality as compared to other algorithms.
Non-Classical Rules in Quantum Games
Authors: Frąckiewicz Piotr
Published: May 2021

Over the last twenty years, quantum game theory has given us many ideas of how quantum games could be played. One of the most prominent ideas in the field is a model of quantum playing bimatrix games introduced by J. Eisert, M. Wilkens and M. Lewenstein. The scheme assumes that players’ strategies are unitary operations and the players act on the maximally entangled two-qubit state. The quantum nature of the scheme has been under discussion since the article by Eisert et al. came out. The aim of our paper was to identify some of non-classical features of the quantum scheme.
3D-Printed Quasi-Absolute Electromagnetic Encoders for Chipless-RFID and Motion Control Applications
Authors: Paredes Ferran , Herrojo Cristian , Martín Ferran
Published: May 2021

This paper presents electromagnetic encoders useful for chipless-RFID and motion control applications. The encoders consist in a pair of linear chains of rectangular apertures implemented by means of 3D printing. One of these chains is periodic and acts as a clock, whereas the other chain contains an identification (ID) code. With these two aperture chains, the ID code can be synchronously read, so that the relative velocity between the tag and the reader is irrelevant. Additionally, it is shown in the paper that by properly designing the reader, it is possible to determine the motion direction. The sensitive part of the reader is a microstrip line loaded with three complementary split ring resonators (CSRRs) etched in the ground plane and fed by three harmonic signals. By encoder motion, the characteristics of the local medium surrounding the CSRRs are modified, and the harmonic signals are amplitude modulated (AM) at the output port of the line, thereby providing the clock signal (which gives the encoder velocity), the ID code (providing also the quasi-absolute position) and the direction of motion. A fabricated prototype encoder is characterized by reading it with a dedicated reader.
Design Strategies and Architectures for Ultra-Low-Voltage Delta-Sigma ADCs
Authors: Benvenuti Lorenzo , Catania Alessandro , Manfredini Giuseppe , Ria Andrea , Piotto Massimo , Bruschi Paolo
Published: May 2021

The design of ultra-low voltage analog CMOS integrated circuits requires ad hoc solutions to counteract the severe limitations introduced by the reduced voltage headroom. A popular approach is represented by inverter-based topologies, which however may suffer from reduced finite DC gain, thus limiting the accuracy and the resolutions of pivotal circuits like analog-to-digital converters. In this work, we discuss the effects of finite DC gain on ultra-low voltage ΔΣ modulators, focusing on the converter gain error. We propose an ultra-low voltage, ultra-low power, inverter-based ΔΣ modulator with reduced finite-DC-gain sensitivity. The modulator employs a two-stage, high DC-gain, switched-capacitor integrator that applies a correlated double sampling technique for offset cancellation and flicker noise reduction; it also makes use of an amplifier that implements a novel common-mode stabilization loop. The modulator was designed with the UMC 0.18 μm CMOS process to operate with a supply voltage of 0.3 V. It was validated by means of electrical simulations using the CadenceTM design environment. The achieved SNDR was 73 dB, with a bandwidth of 640 Hz, and a clock frequency of 164 kHz, consuming only 200.5 nW. It achieves a Schreier Figure of Merit of 168.1 dB. The proposed modulator is also able to work with lower supply voltages down to 0.15 V with the same resolution and a lower power consumption despite of a lower bandwidth. These characteristics make this design very appealing in sensor interfaces powered by energy harvesting sources.
Power Electric Transformer Fault Diagnosis Based on Infrared Thermal Images Using Wasserstein Generative Adversarial Networks and Deep Learning Classifier
Authors: Fanchiang Kuo-Hao , Huang Yen-Chih , Kuo Cheng-Chien
Published: May 2021

The safety of electric power networks depends on the health of the transformer. However, once a variety of transformer failure occurs, it will not only reduce the reliability of the power system but also cause major accidents and huge economic losses. Until now, many diagnosis methods have been proposed to monitor the operation of the transformer. Most of these methods cannot be detected and diagnosed online and are prone to noise interference and high maintenance cost that will cause obstacles to the real-time monitoring system of the transformer. This paper presents a full-time online fault monitoring system for cast-resin transformer and proposes an overheating fault diagnosis method based on infrared thermography (IRT) images. First, the normal and fault IRT images of the cast-resin transformer are collected by the proposed thermal camera monitoring system. Next is the model training for the Wasserstein Autoencoder Reconstruction (WAR) model and the Differential Image Classification (DIC) model. The differential image can be acquired by the calculation of pixel-wise absolute difference between real images and regenerated images. Finally, in the test phase, the well-trained WAR and DIC models are connected in series to form a module for fault diagnosis. Compared with the existing deep learning algorithms, the experimental results demonstrate the great advantages of the proposed model, which can obtain the comprehensive performance with lightweight, small storage size, rapid inference time and adequate diagnostic accuracy.
Technical Solutions of Forest Machine Hybridization
Authors: Mergl Václav , Pandur Zdravko , Klepárník Jan , Kopseak Hrvoje , Bačić Marin , Šušnjar Marijan
Published: May 2021

The paper deals with the characteristics of three different types of power train hybridization of forest logging machines and with the benefits of reducing environmental impacts by comparing new technology with more conventional, older technology. New hybridization options that could be implemented in forestry machines are also discussed. The paper divides a hybrid solution into three classes based on the energy used in the system of hybridization. First is an electro-hybrid system that uses an electric motor and battery or different storage device. The second, a hydraulic hybrid system, is a solution with a hydraulic accumulator, hydraulic motor, and pump. The third system is a combination of the electro-hybrid and hydraulic-hybrid system. The current technical and technological development of hybrid drive systems, as well as their components, has led to significant improvements in drive performance and thus better performance of the new generation of forest vehicles. Improved energy efficiency using hybrid propulsion systems in forest vehicles would result in a significant reduction in greenhouse gas emissions and possibly lower maintenance costs.
Improvement in Performance of the Sulphuric Acid Transport Installation through Modification of Flow Characteristics Using Electric Actuators
Authors: Gil Stanisław , Wnęk Mariusz , Łudzień Robert
Published: May 2021

The paper presents a flow model of the installation for tank car filling with sulphuric acid (VI). For the mathematical modelling of the flow process in the industrial installation, a model diagram of the object and experiments carried out during the installation work were applied. The analysis of specific experimental series showed unusual shapes of mass flow characteristics vs. the valve opening level (too wide hysteresis), which markedly deviated from typical flow characteristics that are found in standards and literature. As a result of flow modelling in the sulphuric acid installation, a better-shaped characteristic was presented that should be obtained based on the measuring system values in the absence of the inertia-related error. In the experiments carried out according to the selected procedures, effects of the actuator speed on the characteristics of interest were determined. It was observed that the use of a “slower” actuator (i.e., longer transition time) narrows the characteristics in terms of a reduced difference in flow readings between the valve opening and closing courses. In the specified research procedure for the real flow characteristics, effects of the measurement transducer dynamics in the flow meter on the characteristic course were assessed. It was demonstrated that a faster transducer enabled closer flow recordings to the valve nominal characteristics. The variable speed can improve the positioning precision, with the use of the lowest possible speed rule directly before the positioning point.
The Energy Situation in Central Asia: A Comprehensive Energy Review Focusing on Rural Areas
Authors: Mehta Kedar , Ehrenwirth Mathias , Trinkl Christoph , Zörner Wilfried , Greenough Rick
Published: May 2021

The northern part of the globe is dominated by industrialisation and is well-developed. For many years, the southern part of the world (South Asia, Africa etc.) has been a target of research concentrating on access to energy (mainly electricity) in rural regions. However, the Central Asian region has not been a focus of energy research compared to South East Asia and Africa. Despite plentiful domestically available energy resources, the energy supply in Central Asia is very unevenly distributed between urban and rural areas. Almost half of the total population of Central Asia lives in rural areas and there is a lack of access to modern energy services to meet primary needs. To analyse the energy situation (i.e., electricity, heating, hot water consumption, cooking, etc.) in rural Central Asia, this paper reviews residential energy consumption trends in rural Central Asian regions as compared to urban areas. Furthermore, the paper illustrates the potential of renewable energies in Central Asia. To perform the study, a qualitative comparative analysis was conducted based on a literature review, data, and statistical information. In summary, the presented article discusses the rural energy situation analytically and provides in-depth insights of Central Asian energy infrastructure.
Methodology for the Correction of the Spatial Orientation Angles of the Unmanned Aerial Vehicle Using Real Time GNSS, a Shoreline Image and an Electronic Navigational Chart
Authors: Naus Krzysztof , Szymak Piotr , Piskur Paweł , Niedziela Maciej , Nowak Aleksander
Published: May 2021

Undoubtedly, Low-Altitude Unmanned Aerial Vehicles (UAVs) are becoming more common in marine applications. Equipped with a Global Navigation Satellite System (GNSS) Real-Time Kinematic (RTK) receiver for highly accurate positioning, they perform camera and Light Detection and Ranging (LiDAR) measurements. Unfortunately, these measurements may still be subject to large errors-mainly due to the inaccuracy of measurement of the optical axis of the camera or LiDAR sensor. Usually, UAVs use a small and light Inertial Navigation System (INS) with an angle measurement error of up to 0.5∘ (RMSE). The methodology for spatial orientation angle correction presented in the article allows the reduction of this error even to the level of 0.01∘ (RMSE). It can be successfully used in coastal and port waters. To determine the corrections, only the Electronic Navigational Chart (ENC) and an image of the coastline are needed.
Assessment of Ponderosa Pine Vigor Using Four-Band Aerial Imagery in South Central Oregon: Crown Objects to Landscapes
Authors: Schrader-Patton Charlie , Grulke Nancy , Bienz Craig
Published: May 2021

Ponderosa pine is an integral part of the forested landscape in the western US; it is the dominant tree species on landscapes that provide critical ecosystem services. Moderate drought tolerance allows it to occupy the transition zone between forests, open woodlands, and grasslands. Increases in stand density resulting from wildfire suppression, combined with lengthening, intensifying, and more frequent droughts have resulted in reduced tree vigor and stand health in dry ponderosa pine throughout its range. To address a management need for efficient landscape-level surveys of forest health, we used Random Forests to develop an object-oriented classification of individual tree crowns (ITCs) into vigor classes using existing, agency-acquired four-band aerial imagery. Classes of tree vigor were based on quantitative physiological and morphological attributes established in a previous study. We applied our model across a landscape dominated by ponderosa pine with a variety of forest treatments to assess their impacts on tree vigor and stand health. We found that stands that were both thinned and burned had the lowest proportion of low-vigor ITCs, and that stands treated before the 2014–2016 drought had lower proportions of low-vigor ITCs than stands treated more recently (2016). Upland stands had significantly higher proportions of low-vigor trees than lowland stands. Maps identifying the low-vigor ITCs would assist managers in identifying priority stands for treatment and marking trees for harvest or retention. These maps can be created using already available imagery and GIS software.
Contrasting Litter Nutrient and Metal Inputs and Soil Chemistry among Five Common Eastern North American Tree Species
Authors: Ott Neil F. J. , Watmough Shaun A.
Published: May 2021

Forest composition has been altered throughout Eastern North America, and changes in species dominance may alter nutrient cycling patterns, influencing nutrient availability and distribution in soils. To assess whether nutrients and metals in litterfall and soil differed among sites influenced by five common Ontario tree species (balsam fir (Abies balsamea (L.) Mill.), eastern hemlock (Tsuga canadensis (L.) Carr.), white pine (Pinus strobus L.), sugar maple (Acer saccharum Marsh.), and yellow birch (Betula alleghaniensis Britt.)), litterfall and soil chemistry were measured at a managed forest in Central Ontario, Canada. Carbon (C) and macronutrient (nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg)) inputs in litterfall varied significantly among sites, primarily due to differences in litterfall mass, which was greatest in deciduous-dominated sites, while differences in elemental concentrations played relatively minor roles. Trace metal inputs in litterfall also varied, with much higher zinc (Zn) and cadmium (Cd) in litterfall within yellow birch dominated stands. Mineral soil oxide composition was very similar among sites, suggesting that differences in soil chemistry were influenced by forest composition rather than parent material. Litter in deciduous-dominated stands had lower C/N, and soils were less acidic than conifer-dominated sites. Deciduous stands also had much shorter elemental residence times in the organic horizons, especially for base cations (Ca, Mg, K) compared with conifer-dominated sites, although total soil nutrient pools were relatively consistent among sites. A change from stands with greater conifer abundance to mixed hardwoods has likely led to more rapid cycling of elements in forests, particularly for base cations. These differences are apparent at small scales (100 m2) in mixed forests that characterize many forested regions in Eastern North America and elsewhere.
Forest Development over a Twenty-Year Chronosequence of Reforested Urban Sites
Authors: Sena Kenton L. , Hackworth Zachary J. , Lhotka John M.
Published: May 2021

Urbanization causes a variety of negative ecological impacts, impairing forests, streams, and other ecosystems. While urban forests are the subject of increasing research attention, planted urban forests are less well-understood than remnant forests; however, these systems may be distinct in terms of ecosystem structure and function. The current study investigates a chronosequence of reforested urban sites in Lexington, KY, USA, with a focus on overstory and understory woody plant community characteristics. Monitoring plots were established in each of the 20 sites; tree height, dbh, and species were surveyed for the overstory, and species and height were surveyed for the understory. Canopy height increased non-linearly with time since planting, rapidly increasing in years 6–10, but remaining relatively steady after year 15. While the overstory was dominated by planted native species, the understory was predominately non-native species, some of which are considered invasive. Overall, the nonlinear logistic relationship of canopy height to time since planting may be driven by species-specific effects—with trees such as ash (Fraxinus spp. L.) and black locust (Robinia pseudoacacia L.) important during the early years after planting, but declining over time due to pests and other pressures. Alternatively, this complex relationship of canopy height with time may be driven by uncontrolled factors such as site quality, landscape position, planted species, etc. The significance of invasive species in the understory suggests that the long-term health of these sites will be compromised without intentional ongoing maintenance to ensure continued forest development toward desired native community characteristics.
Theoretical Development of Plant Root Diameter Estimation Based on GprMax Data and Neural Network Modelling
Authors: Liang Hao , Fan Guoqiu , Li Yinghang , Zhao Yandong
Published: May 2021

The in situ non-destructive quantitative observation of plant roots is difficult. Traditional detection methods are not only time-consuming and labor-intensive, but also destroy the root environment. Ground penetrating radar (GPR), as a non-destructive detection method, has great potential in the estimation of root parameters. In this paper, we use GprMax software to perform forward modeling of plant roots under different soil dielectric constants, and analyze the situation of plant roots with different dielectric constants and different root diameters under 1.5 GHz frequency antenna detection. Firstly, root systems with increasing diameter under different values of root and soil dielectric constant were scanned. Secondly, from the scanning results, two time points T1 and T2 of radar wave entering and penetrating the root system were defined, and the correlation between root diameter D and time interval ΔT between T1 and T2 was analyzed. Finally, the least square regression model and back propagation (BP) neural network model for root diameter parameter estimation were established, and the estimation effects of the two models were compared and evaluated. The research results show that the root diameter (12–48 mm) is highly correlated with the time interval. Given the dielectric constants of the root and soil, the prediction results of the two models are accurate, but the prediction result of the neural network model is more stable, and the residual between the predicted value and the actual value is mainly concentrated in the [−1.5 mm, 1.5 mm] range, as well as the average of prediction error percentage being 3.62%. When the dielectric constants of the root and soil are unknown, the accuracy of the prediction results of the two models is decreased, but the stability of the neural network model is still superior to the least squares model, and the residual error is mainly concentrated in the range of [−5.3 mm, 5.0 mm], the average of prediction error percentage is 10.19%. This study uses GprMax to simulate root system detection and reveals the theoretical potential of GPR technology for non-destructive estimation of root diameter parameters. It is also pointed out that in the field exploration process, if the dielectric constants of the root and soil in the experimental site are sampled and measured first, the prediction accuracy of the model for root diameter would be effectively improved. This research is based on simulation experiments, so further simulation followed by laboratory and field testing is warranted using non-uniform roots and soil.
Dropout Intention, Motivation, and Socio-Demographics of Forestry Students in Romania
Authors: Cocoradă Elena , Curtu Alexandru Lucian , Năstasă Laura Elena , Vorovencii Iosif
Published: May 2021

Reducing students’ dropout rate is a major challenge for universities. Based on a quantitative approach, this study aims to analyze and explain students’ dropout intention at the largest forestry school in Romania by collecting data in their first two months of the academic year prior to failing any courses. The study participants are undergraduate freshmen, male and female students, and the tools are psychological instruments commonly used or specifically developed for this study. Our findings partly confirm previous results, such as the higher intention of dropping out among male students compared to females, and also among students with admission GPA lower or equal to seven, on a GPA scale from 1 to 10, and among students from families with low income. Dropout intention can be explained by the academic performance expectancy, gender, family, socio-economic status, and perceived barriers to completion of studies. There were no differences regarding whether students were from urban or rural areas, proximity of university to home, and high school academic program. Our results may help to implement efficient measures for increasing student retention.
MTOR Signaling and Metabolism in Early T Cell Development
Authors: Werlen Guy , Jain Ritika , Jacinto Estela
Published: May 2021

The mechanistic target of rapamycin (mTOR) controls cell fate and responses via its functions in regulating metabolism. Its role in controlling immunity was unraveled by early studies on the immunosuppressive properties of rapamycin. Recent studies have provided insights on how metabolic reprogramming and mTOR signaling impact peripheral T cell activation and fate. The contribution of mTOR and metabolism during early T-cell development in the thymus is also emerging and is the subject of this review. Two major T lineages with distinct immune functions and peripheral homing organs diverge during early thymic development; the αβ- and γδ-T cells, which are defined by their respective TCR subunits. Thymic T-regulatory cells, which have immunosuppressive functions, also develop in the thymus from positively selected αβ-T cells. Here, we review recent findings on how the two mTOR protein complexes, mTORC1 and mTORC2, and the signaling molecules involved in the mTOR pathway are involved in thymocyte differentiation. We discuss emerging views on how metabolic remodeling impacts early T cell development and how this can be mediated via mTOR signaling.
The Genotoxic and Pro-Apoptotic Activities of Advanced Glycation End-Products (MAGE) Measured with Micronuclei Assay Are Inhibited by Their Low Molecular Mass Counterparts
Authors: Czech Monika , Konopacka Maria , Rogoliński Jacek , Maniakowski Zbigniew , Staniszewska Magdalena , Łaczmański Łukasz , Witkowska Danuta , Gamian Andrzej
Published: May 2021

An association between the cancer invasive activities of cells and their exposure to advanced glycation end-products (AGEs) was described early in some reports. An incubation of cells with BSA–AGE (bovine serum albumin–AGE), BSA–carboxymethyllysine and BSA–methylglyoxal (BSA–MG) resulted in a significant increase in DNA damage. We examined the genotoxic activity of new products synthesized under nonaqueous conditions. These were high molecular mass MAGEs (HMW–MAGEs) formed from protein and melibiose and low molecular mass MAGEs (LMW–MAGEs) obtained from the melibiose and N-α-acetyllysine and N-α-acetylarginine. We have observed by measuring of micronuclei in human lymphocytes in vitro that the studied HMW–MAGEs expressed the genotoxicity. The number of micronuclei (MN) in lymphocytes reached 40.22 ± 5.34 promille (MN/1000CBL), compared to 28.80 ± 6.50 MN/1000 CBL for the reference BSA–MG, whereas a control value was 20.66 ± 1.39 MN/1000CBL. However, the LMW–MAGE fractions did not induce micronuclei formation in the culture of lymphocytes and partially protected DNA against damage in the cells irradiated with X-ray. Human melanoma and all other studied cells, such as bronchial epithelial cells, lung cancer cells and colorectal cancer cells, are susceptible to the genotoxic effects of HMW–MAGEs. The LMW–MAGEs are not genotoxic, while they inhibit HMW–MAGE genotoxic activity. With regard to apoptosis, it is induced with the HMW–MAGE compounds, in the p53 independent way, whereas the low molecular mass product inhibits the apoptosis induction. Further investigations will potentially indicate beneficial apoptotic effect on cancer cells.
Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums?
Authors: Akbarzadeh Mehrdad , Van Laere Katrijn , Leus Leen , De Riek Jan , Van Huylenbroeck Johan , Werbrouck Stefaan P.O. , Dhooghe Emmy
Published: May 2021

Breeding programs in ornamentals can be facilitated by integrating knowledge of phylogenetic relatedness of potential parents along with other genomic information. Using AFLP, genetic distances were determined for 59 Geranium genotypes, comprising 55 commercial cultivars of the three subgenera of a total collection of 61 Geranium genotypes. A subgroup of 45 genotypes, including intragroup and intergroup hybrids, were selected and further characterized for genome sizes and chromosome numbers. The variation in genome size ranged from 1.51 ± 0.01 pg/2C to 12.94 ± 0.07 pg/2C. The chromosome numbers ranged from 26 to 108–110 with some hybrids showing an aberrant number of chromosomes based on their parents’ constitution. All chromosome numbers of Geranium are an even number, which presumes that unreduced gametes occur in some cross combinations. Overall, parental difference in genome size and chromosome number were not limiting for cross compatibility. Good crossing compatibility was correlated to a Jaccard similarity coefficient as parameter for parental relatedness of about 0.5. Additionally, parent combinations with high differences in the DNA/chromosome value could not result in a successful cross. We expect that our results will enable breeding programs to overcome crossing barriers and support further breeding initiatives.
Analysis of Skin Pigmentation and Genetic Ancestry in Three Subpopulations from Pakistan: Punjabi, Pashtun, and Baloch
Authors: Shan Muhammad Adnan , Meyer Olivia Strunge , Refn Mie , Morling Niels , Andersen Jeppe Dyrberg , Børsting Claus
Published: May 2021

Skin pigmentation is one of the most prominent and variable phenotypes in humans. We compared the alleles of 163 SNPs and indels from the Human Pigmentation (HuPi) AmpliSeq™ Custom panel, and biogeographic ancestry with the quantitative skin pigmentation levels on the upper arm, lower arm, and forehead of 299 Pakistani individuals from three subpopulations: Baloch, Pashtun, and Punjabi. The biogeographic ancestry of each individual was estimated using the Precision ID Ancestry Panel. All individuals were mainly of mixed South-Central Asian and European ancestry. However, the Baloch individuals also had an average proportion of Sub-Saharan African ancestry of approximately 10%, whereas it was <1% in the Punjabi and Pashtun individuals. The pairwise genetic distances between the Pashtun, Punjabi, and Baloch subpopulations based on the ancestry markers were statistically significantly different. Individuals from the Pashtun subpopulation had statistically significantly lower skin pigmentation than individuals from the Punjabi and Baloch subpopulations (p < 0.05). The proportions of European and Sub-Saharan African ancestry and five SNPs (rs1042602, rs10831496, rs1426654, rs16891982, and rs12913832) were statistically significantly associated with skin pigmentation at either the upper arm, lower arm or forehead in the Pakistani population after correction for multiple testing (p < 10−3). A model based on four of these SNPs (rs1426654, rs1042602, rs16891982, and rs12913832) explained 33% of the upper arm skin pigmentation. The four SNPs and the proportions of European and Sub-Saharan African ancestry explained 37% of the upper arm skin pigmentation. Our results indicate that the four likely causative SNPs, rs1426654, rs1042602, rs16891982, and rs12913832 located in SLC24A5, TYR, SLC45A2, and HERC2, respectively, are essential for skin color variation in the admixed Pakistani subpopulations.
Mapping of Quantitative Trait Loci Controlling Egg-Quality and -Production Traits in Japanese Quail (Coturnix japonica) Using Restriction-Site Associated DNA Sequencing
Authors: Haqani Mohammad Ibrahim , Nomura Shigeru , Nakano Michiharu , Goto Tatsuhiko , Nagano Atsushi J. , Takenouchi Atsushi , Nakamura Yoshiaki , Ishikawa Akira , Tsudzuki Masaoki
Published: May 2021

This research was conducted to identify quantitative trait loci (QTL) associated with egg-related traits by constructing a genetic linkage map based on single nucleotide polymorphism (SNP) markers using restriction-site associated DNA sequencing (RAD-seq) in Japanese quail. A total of 138 F2 females were produced by full-sib mating of F1 birds derived from an intercross between a male of the large-sized strain with three females of the normal-sized strain. Eggs were investigated at two different stages: the beginning stage of egg-laying and at 12 weeks of age (second stage). Five eggs were analyzed for egg weight, lengths of the long and short axes, egg shell strength and weight, yolk weight and diameter, albumen weight, egg equator thickness, and yolk color (L*, a*, and b* values) at each stage. Moreover, the age at first egg, the cumulative number of eggs laid, and egg production rate were recorded. RAD-seq developed 118 SNP markers and mapped them to 13 linkage groups using the Map Manager QTX b20 software. Markers were spanned on 776.1 cM with an average spacing of 7.4 cM. Nine QTL were identified on chromosomes 2, 4, 6, 10, 12, and Z using the simple interval mapping method in the R/qtl package. The QTL detected affected 10 egg traits of egg weight, lengths of the long and short axes of egg, egg shell strength, yolk diameter and weight, albumen weight, and egg shell weight at the beginning stage, yellowness of the yolk color at the second stage, and age at first egg. This is the first report to perform a quail QTL analysis of egg-related traits using RAD-seq. These results highlight the effectiveness of RAD-seq associated with targeted QTL and the application of marker-assisted selection in the poultry industry, particularly in the Japanese quail.
Exploration of Alternative Splicing Events in Mesenchymal Stem Cells from Human Induced Pluripotent Stem Cells
Authors: Jeong Ji-Eun , Seol Binna , Kim Han-Seop , Kim Jae-Yun , Cho Yee-Sook
Published: May 2021

Although comparative genome-wide transcriptomic analysis has provided insight into the biology of human induced pluripotent stem cell-derived mesenchymal stem cells (iMSCs), the distinct alternative splicing (AS) signatures of iMSCs remain elusive. Here, we performed Illumina RNA sequencing analysis to characterize AS events in iMSCs compared with tissue-derived MSCs. A total of 4586 differentially expressed genes (|FC| > 2) were identified between iMSCs and umbilical cord blood-derived MSCs (UCB-MSCs), including 2169 upregulated and 2417 downregulated genes. Of these, 164 differentially spliced events (BF > 20) in 112 genes were identified between iMSCs and UCB-MSCs. The predominant type of AS found in iMSCs was skipped exons (43.3%), followed by retained introns (19.5%), alternative 3′ (15.2%) and 5′ (12.8%) splice sites, and mutually exclusive exons (9.1%). Functional enrichment analysis showed that the differentially spliced genes (|FC| > 2 and BF > 20) were mainly enriched in functions associated with focal adhesion, extracellular exosomes, extracellular matrix organization, cell adhesion, and actin binding. Splice isoforms of selected genes including TRPT1, CNN2, and AP1G2, identified in sashimi plots, were further validated by RT-PCR analysis. This study provides valuable insight into the biology of iMSCs and the translation of mechanistic understanding of iMSCs into therapeutic applications.
Exploring Combined Influences of Seasonal East Atlantic (EA) and North Atlantic Oscillation (NAO) on the Temperature-Precipitation Relationship in the Iberian Peninsula
Authors: Rodrigo Fernando S.
Published: May 2021

The combined influence of the North Atlantic Oscillation (NAO) and the East Atlantic (EA) patterns on the covariability of temperatures and precipitation in 35 stations of the Iberian Peninsula during the period 1950–2019 is analysed in this work. Four EA-NAO composites were defined from teleconnection patterns’ positive and negative phases: EA+NAO+, EA+NAO-, EA-NAO+ and EA-NAO-. Daily data of maximum and minimum temperature were used to obtain seasonal means (TX and TN, respectively), and the covariability of these variables with accumulated seasonal rainfall (R) was studied comparing results obtained for different NAO and EA composites. Main results indicate slight differences in the spatial coverage of correlation coefficients between R and temperature variables, except in spring when the generalised negative relationship between R and TX under EA+NAO+ and EA-NAO- disappears under EA-NAO+ and EA+NAO- composites. This result may be useful to interpret and discuss historical reconstructions of the Iberian climate.