bluuebunny/crossref_metadata_2025 huggingface dataset:
Mild Retinopathy Is a Risk Factor for Cardiovascular Mortality in Japanese With and Without Hypertension
Authors: Sairenchi Toshimi , Iso Hiroyasu , Yamagishi Kazumasa , Irie Fujiko , Okubo Yoshiro , Gunji Junko , Muto Takashi , Ota Hitoshi
Published: November 2011

Background—

It is unclear whether mild hypertensive retinopathy is a risk factor for mortality. This study examined whether mild hypertensive retinopathy could be a risk factor for cardiovascular mortality in subjects with and without hypertension.

Methods and Results—

In this cohort study, 87 890 individuals (29 917 men and 57 973 women) 40 to 79 years of age in 1993 were followed up until 2008. Retinal photography was classified as normal, grade 1, or grade 2 based on the Keith-Wagener-Barker system. Risk ratios for all-cause and cause-specific mortality for each classification were calculated with Cox proportional hazards regression models. Covariates included age, systolic blood pressure, antihypertensive medication use, and other cardiovascular risk factors. Multivariable hazard ratios for total cardiovascular disease mortality were 1.24 (95% confidence interval [CI], 1.12–1.38) and 1.23 (95% CI, 1.03–1.47) for grades 1 and 2 among men and 1.12 (95% CI, 1.01–1.24) and 1.44 (95% CI, 1.24–1.68) for grades 1 and 2 among women, respectively. Hazard ratios for total stroke mortality were 1.31 (95% CI, 1.13–1.53) and 1.38 (95% CI, 1.08–1.77) for grades 1 and 2 among men and 1.30 (95% CI, 1.12–1.50) and 1.70 (95% CI, 1.36–2.11) for grades 1 and 2 among women, respectively. For both hypertensive and normotensive subjects of each sex, multivariable hazard ratios for all-cause mortality, total cardiovascular mortality, and total stroke mortality were significantly higher for grade 1 or 2 compared with normal.

Conclusions—

Mild hypertensive retinopathy is a risk factor for cardiovascular mortality independently of cardiovascular risk factors among men and women with and without hypertension.
Left Ventricular Mass and Function With Reduced-Fat or Reduced-Carbohydrate Hypocaloric Diets in Overweight and Obese Subjects
Authors: Haufe Sven , Utz Wolfgang , Engeli Stefan , Kast Petra , Böhnke Jana , Pofahl Martin , Traber Julius , Haas Verena , Hermsdorf Mario , Mähler Anja , Busjahn Andreas , Wiesner Susanne , Otto Christoph , Mehling Heidrun , Luft Friedrich C. , Boschmann Michael , Schulz-Menger Jeanette , Jordan Jens
Published: November 2011

In animals, carbohydrate and fat composition during dietary interventions influenced cardiac metabolism, structure, and function. Because reduced-carbohydrate and reduced-fat hypocaloric diets are commonly used in the treatment of obesity, we investigated whether these interventions differentially affect left ventricular mass, cardiac function, and blood pressure. We randomized 170 overweight and obese subjects (body mass index, 32.9±4.4; range, 26.5–45.4 kg/m
2
) to 6-month hypocaloric diets with either reduced carbohydrate intake or reduced fat intake. We obtained cardiac MRI and ambulatory blood pressure recordings over 24 hours before and after 6 months. Ninety subjects completing the intervention period had a full cardiac MRI data set. Subjects lost 7.3±4.0 kg (7.9±3.8%) with reduced-carbohydrate diet and 6.2±4.2 kg (6.7±4.4%) with reduced-fat diet (
P
<0.001 within each group;
P
=not significant between interventions). Caloric restriction led to similar significant decreases in left ventricular mass with low-carbohydrate diets (5.4±5.4 g) or low-fat diets (5.2±4.8 g;
P
<0.001 within each group;
P
=not significant between interventions). Systolic and diastolic left ventricular function did not change with either diet. The 24-hour systolic blood pressure decreased similarly with both interventions. Body weight change (β=0.33;
P
=0.02) and percentage of ingested n-3 polyunsaturated fatty acids (β=−0.27;
P
=0.03) predicted changes in left ventricular mass. In conclusion, weight loss induced by reduced-fat diets or reduced-carbohydrate diets similarly improved left ventricular mass in overweight and obese subjects over a 6-month period. However, n-3 polyunsaturated fatty acid ingestion may have an independent beneficial effect on left ventricular mass.
Multiscale Waves in an MJO Background and Convective Momentum Transport Feedback
Authors: Khouider Boualem , Han Ying , Majda Andrew J. , Stechmann Samuel N.
Published: November 2011

The authors use linear analysis for a simple model to study the evolution of convectively coupled waves (CCWs) in a background shear and background moisture mimicking the observed structure of the Madden–Julian oscillation (MJO). This is motivated by the observation, in an idealized setting, of intraseasonal two-way interactions between CCWs and a background wind. It is found here that profiles with a bottom-heavy moisture content are more favorable to the development of mesoscale/squall line–like waves whereas synoptic-scale CCWs are typically more sensitive to the shear strength. The MJO envelope is thus divided into three regions, in terms of the types of CCWs that are favored: an onset region in front that is favorable to Kelvin waves, a mature or active region in the middle in which squall lines are prominent, and the stratiform and decay phase region in the back that is favorable to westward inertia–gravity (WIG) waves. A plausible convective momentum transport (CMT) feedback is then provided according to the results of the idealized two-way interaction model. The active region, in particular, coincides with the westerly wind burst where both Kelvin waves and squall lines are believed to play a significant role in both the deceleration of low-/high-level easterly/westerly winds and the acceleration of low-/high-level westerly/easterly winds. The WIG waves in the wake could be a precursor for a subsequent MJO event through the acceleration of low-/high-level easterly/westerly winds, which in turn favor Kelvin waves, and the cycle repeats. These results open interesting directions for future studies using observations and/or detailed numerical simulations using the full primitive equation.
Free the Victim: A Critique of the Western Conception of Victimhood
Authors: Van Dijk Jan
Published: November 2011

In Western languages those affected by crime are universally labelled as ‘victims’, meaning the sacrificed ones. According to the author this practice seems to originate from the association of the plight of victims with the suffering of Jesus Christ. In his view, the victim label, although eliciting compassion for victims, assigns to them a social role of passivity and forgiveness that they may increasingly find to be restraining. He analyses the narratives of eleven high-profile victims such as Natascha Kampusch, the couple McCann and Reemtsma to illustrate this thesis. The article continues with a critical review of biases deriving from the unreflexive adoption of the victim label in various schools of thought in victimology and criminal law. Finally, the author argues for the introduction of stronger procedural rights for crime victims in criminal trials and for a new focus within victimology on processes of victim labelling.
Crime Victims and their Social Network: A Field Study on the Cognitive Effects of Victimisation, Attributional Responses and the Victim-Blaming Model
Authors: Winkel Frans Willem , Denkers Adriaan
Published: November 2011

A field study is reported focusing on the cognitive effects of criminal victimisation, on the types of responses victims encounter in their social network, and on the validity of the ‘victim blaming model’. Data reveal that victimisations have a negative impact on the perceived benevolence of the world. Perceptions of self control and control over outcomes are reduced, and victims consider themselves less as persons having luck in life. These cognitive effects appear to stretch out beyond those directly involved. Results generally do not support the theoretically dominant position in the victimological literature on ‘blaming the victim’. Empathical responses and external attributions from the social network are much more common than internal responses to victimisation. Moreover, internal attributions from the social network appear to work more positively on the wellbeing of victims than external attributions. These outcomes - which are in line with the Janoff-Bulman model - are clearly in contrast to the ‘victim blaming model’. Some implications for future studies are discussed.
Fear of Crime in Belgrade
Authors: Nikolic-Ristanovic Vesna
Published: November 2011

This paper reports research findings from the capital city of Yugoslavia, Belgrade. Both fear of crime and opportunity theory suggestions were tested. Differences in victimization experience influencing day/night time fear of crime, as well as differences in day/night time fear of crime influences on routine activities, were examined. Other factors influencing fear of specific crimes were examined.

The research was carried out on a random sample comprising 400 respondents living in four Belgrade municipalities. The results of this research show that there exists a feeling of unsafeness in relation to criminal behavior. This feeling, of fear of crime, is more strongly felt at night than in daytime, and to a large extent represents a reflection of the objective risk of victimization. This is especially true of people who indicated fear of daytime crime. People tend to constrain and/or change their routine activities as a consequence of fear of crime; this tendency positively correlates with their level of fear. People who are afraid of daytime crime are more often constrained in their behavior than are those who fear night time crime.
Lessons in Leveraging Implementation
Authors: Furgol Katherine E. , Helms Lelia B.
Published: November 2011

Through a case study that highlights the role of rulemaking in the evolution of the No Child Left Behind Act (NCLB), this article provides strategies for educators who seek both to influence policy outcomes and to understand the dynamics of policymaking. This study examines the regulatory processes for NCLB, specifically related to the adoption of growth models, at both the federal and state levels, augmenting the slim body of literature on rulemaking in educational policy. It focuses on rulemaking processes as they define and redirect the substance of policy, create ongoing opportunities for stakeholder participation, and influence, and tailor policies to the state and local settings in which delivery occurs. This article describes policy as cumulative, increasingly complex and multileveled. It recommends that stakeholders monitor and target federal and state rulemaking venues so as to influence and adapt legislative mandates to the specific contexts and demands of practice.
Scouting for Soldiers: Reconnaissance and the British Cavalry, 1899—1914
Authors: Jones Spencer
Published: November 2011

Although reconnaissance was considered the primary duty of cavalry, British cavalry were poorly trained in this role prior to the Anglo-Boer War (1899—1902). The dismal performance of scouting duties in this conflict prompted a complete overhaul of reconnaissance organization, while innovative training methods were introduced to improve scouting and horse-mastership. Although the process was not without difficulties, the results were positive and proved extremely valuable in 1914. It is the purpose of this article to add to the ongoing debate on British cavalry in the period 1899—1914 by demonstrating how the vital skills of reconnaissance were developed as a result of the Anglo-Boer War experience.
Outcomes of Asthma Education and Healthy Homes Curriculum Provided Through Community Health Workers in South Texas Colonias
Authors: Zuniga Genny Carrillo , Lucio Rose , Seol Yoon-Ho , Garza Norma , Zuniga Miguel A. , Hernandez Dora , Harrison Whitney
Published: November 2011

Background. Childhood asthma rates are highest among minorities and among those who reside in low-income communities. This is especially true of children who reside in colonias, which are defined as substandard housing communities in irregular rural settlements located along the US-Mexico border. This study aimed to evaluate the knowledge acquired by participants before and after an educational intervention was provided. Methods. This study used the healthy homes training approach, including integrated pest management, identification of asthma triggers, and asthma case management, to increase asthma management awareness and control. This dual training approach combined a healthy homes module with asthma education and was implemented by certified promotoras (community health workers) in South Texas colonias. Promotoras are generally trusted and respected individuals who live in or around the communities in which they work. The promotoras identified and trained 108 parents of children aged between 5 and 12 years in households in Hidalgo County with lifetime asthma diagnosed by his/her physician and of children who may not have been diagnosed by a health professional with asthma but suffer from symptoms of asthma. Results. Asthma management and healthy homes knowledge, including attitudes and practices, significantly improved among parents after the implementation of the dual training program. Of the 108 participants, 78% had never received asthma education, 78% had a child with lifetime asthma diagnosed by his/her physician, and 32% reported having medical insurance. Pretest and posttest comparison results based on 9 out of 14 questions related to asthma triggers and healthy home principles showed a significant improvement in knowledge gained among the participants. Regardless of the study limitations (self-report instrument for the pretest and the posttest), the results showed that families in colonias living with asthma benefit from an integrated training approach.
The relationship between tissue factor and cancer progression: insights from bench and bedside
Authors: van den Berg Yascha W. , Osanto Susanne , Reitsma Pieter H. , Versteeg Henri H.
Published: November 2011

It is now widely recognized that a strong correlation exists between cancer and aberrant hemostasis. Patients with various types of cancers, including pancreatic, colorectal, and gastric cancer, often develop thrombosis, a phenomenon commonly referred to as Trousseau syndrome. Reciprocally, components from the coagulation cascade also influence cancer progression. The primary initiator of coagulation, the transmembrane receptor tissue factor (TF), has gained considerable attention as a determinant of tumor progression. On complex formation with its ligand, coagulation factor VIIa, TF influences protease-activated receptor-dependent tumor cell behavior, and regulates integrin function, which facilitate tumor angiogenesis both in vitro and in mouse models. Furthermore, evidence exists that an alternatively spliced isoform of TF also affects tumor growth and tumor angiogenesis. In patient material, TF expression and TF cytoplasmic domain phosphorylation correlate with disease outcome in many, but not in all, cancer subtypes, suggesting that TF-dependent signal transduction events are a potential target for therapeutic intervention in selected types of cancer. In this review, we summarize our current understanding of the role of TF in tumor growth and metastasis, and speculate on anticancer therapy by targeting TF.
The PI3K/PKB signaling module as key regulator of hematopoiesis: implications for therapeutic strategies in leukemia
Authors: Polak Roel , Buitenhuis Miranda
Published: November 2011

An important mediator of cytokine signaling implicated in regulation of hematopoiesis is the PI3K/protein kinase B (PKB/c-Akt) signaling module. Constitutive activation of this signaling module has been observed in a large group of leukemias. Because activation of this signaling pathway has been demonstrated to be sufficient to induce hematologic malignancies and is thought to correlate with poor prognosis and enhanced drug resistance, it is considered to be a promising target for therapy. A high number of pharmacologic inhibitors directed against either individual or multiple components of this pathway have already been developed to improve therapy. In this review, the safety and efficacy of both single and dual-specificity inhibitors will be discussed as well as the potential of combination therapy with either inhibitors directed against other signal transduction molecules or classic chemotherapy.
Proteomic and biochemical analyses reveal the activation of unfolded protein response, ERK-1/2 and ribosomal protein S6 signaling in experimental autoimmune myocarditis rat model
Authors: Chung Joo Hee , Choi Hee Jung , Kim Soo Young , Hong Kwan Soo , Min Soo Kee , Nam Myung Hee , Kim Chan Wha , Koh Young Ho , Seo Jong Bok
Published: November 2011

Background

To investigate the molecular and cellular pathogenesis underlying myocarditis, we used an experimental autoimmune myocarditis (EAM)-induced heart failure rat model that represents T cell mediated postinflammatory heart disorders.

Results

By performing unbiased 2-dimensional electrophoresis of protein extracts from control rat heart tissues and EAM rat heart tissues, followed by nano-HPLC-ESI-QIT-MS, 67 proteins were identified from 71 spots that exhibited significantly altered expression levels. The majority of up-regulated proteins were confidently associated with unfolded protein responses (UPR), while the majority of down-regulated proteins were involved with the generation of precursor metabolites and energy metabolism in mitochondria. Although there was no difference in AKT signaling between EAM rat heart tissues and control rat heart tissues, the amounts and activities of extracellular signal-regulated kinase (ERK)-1/2 and ribosomal protein S6 (rpS6) were significantly increased. By comparing our data with the previously reported myocardial proteome of the Coxsackie viruses of group B (CVB)-mediated myocarditis model, we found that UPR-related proteins were commonly up-regulated in two murine myocarditis models. Even though only two out of 29 down-regulated proteins in EAM rat heart tissues were also dysregulated in CVB-infected rat heart tissues, other proteins known to be involved with the generation of precursor metabolites and energy metabolism in mitochondria were also dysregulated in CVB-mediated myocarditis rat heart tissues, suggesting that impairment of mitochondrial functions may be a common underlying mechanism of the two murine myocarditis models.

Conclusions

UPR, ERK-1/2 and S6RP signaling were activated in both EAM- and CVB-induced myocarditis murine models. Thus, the conserved components of signaling pathways in two murine models of acute myocarditis could be targets for developing new therapeutic drugs or methods aimed at treating enigmatic myocarditis.
A 48 SNP set for grapevine cultivar identification
Authors: Cabezas José A , Ibáñez Javier , Lijavetzky Diego , Vélez Dolores , Bravo Gema , Rodríguez Virginia , Carreño Iván , Jermakow Angelica M , Carreño Juan , Ruiz-García Leonor , Thomas Mark R , Martinez-Zapater José M
Published: November 2011

Background

Rapid and consistent genotyping is an important requirement for cultivar identification in many crop species. Among them grapevine cultivars have been the subject of multiple studies given the large number of synonyms and homonyms generated during many centuries of vegetative multiplication and exchange. Simple sequence repeat (SSR) markers have been preferred until now because of their high level of polymorphism, their codominant nature and their high profile repeatability. However, the rapid application of partial or complete genome sequencing approaches is identifying thousands of single nucleotide polymorphisms (SNP) that can be very useful for such purposes. Although SNP markers are bi-allelic, and therefore not as polymorphic as microsatellites, the high number of loci that can be multiplexed and the possibilities of automation as well as their highly repeatable results under any analytical procedure make them the future markers of choice for any type of genetic identification.

Results

We analyzed over 300 SNP in the genome of grapevine using a re-sequencing strategy in a selection of 11 genotypes. Among the identified polymorphisms, we selected 48 SNP spread across all grapevine chromosomes with allele frequencies balanced enough as to provide sufficient information content for genetic identification in grapevine allowing for good genotyping success rate. Marker stability was tested in repeated analyses of a selected group of cultivars obtained worldwide to demonstrate their usefulness in genetic identification.

Conclusions

We have selected a set of 48 stable SNP markers with a high discrimination power and a uniform genome distribution (2-3 markers/chromosome), which is proposed as a standard set for grapevine (Vitis viniferaL.) genotyping. Any previous problems derived from microsatellite allele confusion between labs or the need to run reference cultivars to identify allele sizes disappear using this type of marker. Furthermore, because SNP markers are bi-allelic, allele identification and genotype naming are extremely simple and genotypes obtained with different equipments and by different laboratories are always fully comparable.
Rapid evolution of fluoroquinolone-resistant Escherichia coli in Nigeria is temporally associated with fluoroquinolone use
Authors: Lamikanra Adebayo , Crowe Jennifer L , Lijek Rebeccah S , Odetoyin Babatunde W , Wain John , Aboderin A Oladipo , Okeke Iruka N
Published: November 2011

Background

Antibiotic resistance has necessitated fluoroquinolone use but little is known about the selective forces and resistance trajectory in malaria-endemic settings, where selection from the antimalarial chloroquine for fluoroquinolone-resistant bacteria has been proposed.

Methods

Antimicrobial resistance was studied in fecal Escherichia coli isolates in a Nigerian community. Quinolone-resistance determining regions of gyrA and parC were sequenced in nalidixic acid resistant strains and horizontally-transmitted quinolone-resistance genes were sought by PCR. Antimicrobial prescription practices were compared with antimicrobial resistance rates over a period spanning three decades.

Results

Before 2005, quinolone resistance was limited to low-level nalixidic acid resistance in fewer than 4% of E. coli isolates. In 2005, the proportion of isolates demonstrating low-level quinolone resistance due to elevated efflux increased and high-level quinolone resistance and resistance to the fluoroquinolones appeared. Fluoroquinolone resistance was attributable to single nucleotide polymorphisms in quinolone target genes gyrA and/or parC. By 2009, 35 (34.5%) of isolates were quinolone non-susceptible with nine carrying gyrA and parC SNPs and six bearing identical qnrS1 alleles. The antimalarial chloroquine was heavily used throughout the entire period but E. coli with quinolone-specific resistance mechanisms were only detected in the final half decade, immediately following the introduction of the fluoroquinolone antibacterial ciprofloxacin.

Conclusions

Fluoroquinolones, and not chloroquine, appear to be the selective force for fluoroquinolone-resistant fecal E. coli in this setting. Rapid evolution to resistance following fluoroquinolone introduction points the need to implement resistant containment strategies when new antibacterials are introduced into resource-poor settings with high infectious disease burdens.
Evaluating a streamlined clinical tool and educational outreach intervention for health care workers in Malawi: the PALM PLUS case study
Authors: Sodhi Sumeet , Banda Hastings , Kathyola Damson , Burciul Barry , Thompson Sandy , Joshua Martias , Bateman Eric , Fairall Lara , Martiniuk Alexandra , Cornick Ruth , Faris Gill , Draper Beverley , Mondiwa Martha , Katengeza Egnat , Sanudi Lifah , Zwarenstein Merrick , Schull Michael J
Published: November 2011

Background

Nearly 3 million people in resource-poor countries receive antiretrovirals for the treatment of HIV/AIDS, yet millions more require treatment. Key barriers to treatment scale up are shortages of trained health care workers, and challenges integrating HIV/AIDS care with primary care.

The research

PALM PLUS (Practical Approach to Lung Health and HIV/AIDS in Malawi) is an intervention designed to simplify and integrate existing Malawian national guidelines into a single, simple, user-friendly guideline for mid-level health care workers. Training utilizes a peer-to-peer educational outreach approach. Research is being undertaken to evaluate this intervention to generate evidence that will guide future decision-making for consideration of roll out in Malawi. The research consists of a cluster randomized trial in 30 public health centres in Zomba District that measures the effect of the intervention on staff satisfaction and retention, quality of patient care, and costs through quantitative, qualitative and health economics methods.

Results and outcomes

In the first phase of qualitative inquiry respondents from intervention sites demonstrated in-depth knowledge of PALM PLUS compared to those from control sites. Participants in intervention sites felt that the PALM PLUS tool empowered them to provide better health services to patients. Interim staff retention data shows that there were, on average, 3 to 4 staff departing from the control and intervention sites per month. Additional qualitative, quantitative and economic analyses are planned.

The partnership

Dignitas International and the Knowledge Translation Unit at the University of Cape Town Lung Institute have led the adaptation and development of the PALM PLUS intervention, using experience gained through the implementation of the South African precursor, PALSA PLUS. The Malawian partners, REACH Trust and the Research Unit at the Ministry of Health, have led the qualitative and economic evaluations. Dignitas and Ministry of Health have facilitated interaction with implementers and policy-makers.

Challenges and successes

This initiative is an example of South-South knowledge translation between South Africa and Malawi, mediated by a Canadian academic-NGO hybrid. Our success in developing and rolling out PALM PLUS in Malawi suggests that it is possible to adapt and implement this intervention for use in other resource-limited settings.
A study on the effect of different chemical routes on functionalization of MWCNTs by various groups (-COOH, -SO3H, -PO3H2)
Authors: Kumar Pawan , Park Jin-Soo , Randhawa Prabhsharan , Sharma Sandeep , Shin Mun-Sik , Sekhon Satpal Singh
Published: November 2011

Pristine multiwall carbon nanotubes [MWCNTs] have been functionalized with various groups (-COOH, -SO3H, -PO3H2) using different single- and double-step chemical routes. Various chemical treatments were given to MWCNTs using hydrochloric, nitric, phosphoric, and sulphuric acids, followed by a microwave treatment. The effect of the various chemical treatments and the dispersion using a surfactant via ultrasonication on the functionalization of MWCNTs has been studied. The results obtained have been compared with pristine MWCNTs. Scanning electron microscopy, energy dispersive X-ray [EDX] spectroscopy, and transmission electron microscopy confirm the dispersion and functionalization of MWCNTs. Their extent of functionalization with -SO3H and -PO3H2 groups from the EDX spectra has been observed to be higher for the samples functionalized with a double-step chemical route and a single-step chemical route, respectively. The I
D/I
G ratio calculated from Raman data shows a maximum defect concentration for the sample functionalized with the single-step chemical treatment using nitric acid. The dispersion of MWCNTs with the surfactant, Triton X-100, via ultrasonication helps in their unbundling, but the extent of functionalization mainly depends on the chemical route followed for their treatment. The functionalized carbon nanotubes can be used in proton conducting membranes for fuel cells.
Effective harvesting, detection, and conversion of IR radiation due to quantum dots with built-in charge
Authors: Sablon Kimberly , Sergeev Andrei , Vagidov Nizami , Antipov Andrei , Little John , Mitin Vladimir
Published: November 2011

We analyze the effect of doping on photoelectron kinetics in quantum dot [QD] structures and find two strong effects of the built-in-dot charge. First, the built-in-dot charge enhances the infrared [IR] transitions in QD structures. This effect significantly increases electron coupling to IR radiation and improves harvesting of the IR power in QD solar cells. Second, the built-in charge creates potential barriers around dots, and these barriers strongly suppress capture processes for photocarriers of the same sign as the built-in-dot charge. The second effect exponentially increases the photoelectron lifetime in unipolar devices, such as IR photodetectors. In bipolar devices, such as solar cells, the solar radiation creates the built-in-dot charge that equates the electron and hole capture rates. By providing additional charge to QDs, the appropriate doping can significantly suppress the capture and recombination processes via QDs. These improvements of IR absorption and photocarrier kinetics radically increase the responsivity of IR photodetectors and photovoltaic efficiency of QD solar cells.
Facile preparation of highly-dispersed cobalt-silicon mixed oxide nanosphere and its catalytic application in cyclohexane selective oxidation
Authors: Zhang Qiaohong , Chen Chen , Wang Min , Cai Jiaying , Xu Jie , Xia Chungu
Published: November 2011

Highly dispersed cobalt-silicon mixed oxide [Co-SiO2] nanosphere was successfully prepared with a modified reverse-phase microemulsion method. This material was characterized in detail by X-ray diffraction, transmission electron microscopy, Fourier transform infrared, ultraviolet-visible diffuse reflectance spectra, X-ray absorption spectroscopy near-edge structure, and N2 adsorption-desorption measurements. High valence state cobalt could be easily obtained without calcination, which is fascinating for the catalytic application for its strong oxidation ability. In the selective oxidation of cyclohexane, Co-SiO2 acted as an efficient catalyst, and good activity could be obtained under mild conditions.
Multiple-set split feasibility problems for total asymptotically strict pseudocontractions mappings
Authors: Yang LI , Chang Shih-Sen , Cho Yeol JE , Kim Jong KYU
Published: November 2011

The purpose of this article is to propose and investigate an algorithm for solving the multiple-set split feasibility problems for total asymptotically strict pseu-docontractions mappings in infinite-dimensional Hilbert spaces. The results presented in this article improve and extend some recent results of A. Moudafi, H. K. Xu, Y. Censor, A. Segal, T. Elfving, N. Kopf, T. Bortfeld, X. A. Motova, Q. Yang, A. Gibali, S. Reich and others.

2000 AMS Subject Classification: 47J05; 47H09; 49J25.
Efficient and accurate image alignment using TSK-type neuro-fuzzy network with data-mining-based evolutionary learning algorithm
Authors: Hsu Chi-Yao , Cheng Yi-Chang , Lin Sheng-Fuu
Published: November 2011

Image alignment is considered a key problem in visual inspection applications. The main concerns for such tasks are fast image alignment with subpixel accuracy. About this, neural network-based approaches are very popular in visual inspection because of their high accuracy and efficiency of aligning images. However, such methods are difficult to identify the structure and parameters of neural network. In this study, a Takagi-Sugeno-Kang-type neuro-fuzzy network (NFN) with data-mining-based evolutionary learning algorithm (DMELA) is proposed. Compared with traditional learning algorithms, DMELA combines the self-organization algorithm (SOA), data-mining selection method (DMSM), and regularized least square (RLS) method to not only determine a suitable number of fuzzy rules, but also automatically tune the parameters of NFN. Experimental results are shown to demonstrate superior performance of the DMELA constructed image alignment system over other typical learning algorithms and existing alignment systems. Such system is useful to develop accurate and efficient image alignment systems.
Identification, characterization, and comparative genomic distribution of the HERV-K (HML-2) group of human endogenous retroviruses
Authors: Subramanian Ravi P , Wildschutte Julia H , Russo Crystal , Coffin John M
Published: November 2011

Background

Integration of retroviral DNA into a germ cell may lead to a provirus that is transmitted vertically to that host's offspring as an endogenous retrovirus (ERV). In humans, ERVs (HERVs) comprise about 8% of the genome, the vast majority of which are truncated and/or highly mutated and no longer encode functional genes. The most recently active retroviruses that integrated into the human germ line are members of the Betaretrovirus-like HERV-K (HML-2) group, many of which contain intact open reading frames (ORFs) in some or all genes, sometimes encoding functional proteins that are expressed in various tissues. Interestingly, this expression is upregulated in many tumors ranging from breast and ovarian tissues to lymphomas and melanomas, as well as schizophrenia, rheumatoid arthritis, and other disorders.

Results

No study to date has characterized all HML-2 elements in the genome, an essential step towards determining a possible functional role of HML-2 expression in disease. We present here the most comprehensive and accurate catalog of all full-length and partial HML-2 proviruses, as well as solo LTR elements, within the published human genome to date. Furthermore, we provide evidence for preferential maintenance of proviruses and solo LTR elements on gene-rich chromosomes of the human genome and in proximity to gene regions.

Conclusions

Our analysis has found and corrected several errors in the annotation of HML-2 elements in the human genome, including mislabeling of a newly identified group called HML-11. HML-elements have been implicated in a wide array of diseases, and characterization of these elements will play a fundamental role to understand the relationship between endogenous retrovirus expression and disease.
Helicobacter pylori interferes with an embryonic stem cell micro RNA cluster to block cell cycle progression
Authors: Belair Cédric , Baud Jessica , Chabas Sandrine , Sharma Cynthia M , Vogel Jörg , Staedel Cathy , Darfeuille Fabien
Published: November 2011

Background

MicroRNAs, post-transcriptional regulators of eukaryotic gene expression, are implicated in host defense against pathogens. Viruses and bacteria have evolved strategies that suppress microRNA functions, resulting in a sustainable infection. In this work we report that Helicobacter pylori, a human stomach-colonizing bacterium responsible for severe gastric inflammatory diseases and gastric cancers, downregulates an embryonic stem cell microRNA cluster in proliferating gastric epithelial cells to achieve cell cycle arrest.

Results

Using a deep sequencing approach in the AGS cell line, a widely used cell culture model to recapitulate early events of H. pylori infection of gastric mucosa, we reveal that hsa-miR-372 is the most abundant microRNA expressed in this cell line, where, together with hsa-miR-373, it promotes cell proliferation by silencing large tumor suppressor homolog 2 (LATS2) gene expression. Shortly after H. pylori infection, miR-372 and miR-373 synthesis is highly inhibited, leading to the post-transcriptional release of LATS2 expression and thus, to a cell cycle arrest at the G1/S transition. This downregulation of a specific cell-cycle-regulating microRNA is dependent on the translocation of the bacterial effector CagA into the host cells, a mechanism highly associated with the development of severe atrophic gastritis and intestinal-type gastric carcinoma.

Conclusions

These data constitute a novel example of host-pathogen interplay involving microRNAs, and unveil the couple LATS2/miR-372 and miR-373 as an unexpected mechanism in infection-induced cell cycle arrest in proliferating gastric cells, which may be relevant in inhibition of gastric epithelium renewal, a major host defense mechanism against bacterial infections.
Astrocyte elevated gene 1: biological functions and molecular mechanism in cancer and beyond
Authors: Ying Zhe , Li Jun , Li Mengfeng
Published: November 2011

Since its discovery, nearly one decade of research on astrocyte elevated gene 1 (AEG-1) has witnessed expanding knowledge of this molecule, ranging from its role in cancer biology to molecular mechanisms underlying the biological functions. As a multifunctional oncoprotein, AEG-1 has been shown to overexpress in multiple types of human cancer, and the elevation of AEG-1 in tumor cells leads to enhanced phenotypes characteristic of malignant aggressiveness, including increased abilities to proliferate robustly, to invade surrounding tissues, to migrate, to induce neovascularization, and to enhance chemoresistance. The multifunctional role of AEG-1 in tumor development and progression has been found to be associated with several signaling cascades, namely, 1) activation of NF-kappa B, partially through direct interaction with p65; 2) PI3K/AKT signaling triggered by AEG-1 indirectly; 3) enhancement of the transcriptional activity of beta-catenin by indirect activation of MAPK and induction of LEF1; 4) regulation of mi/siRNA-mediated gene silencing by interacting with SND1; and 5) promotion of protective autophagy; in addition to possibly unknown mechanisms. Elevated AEG-1 expression is seen in nearly all tumor types, and in most cases AEG-1 positively correlates with tumor progression and poorer patient survival. Taken together, AEG-1 might represent a potential prognostic biomarker and therapeutic target.
The dynamics underlying pseudo-plateau bursting in a pituitary cell model
Authors: Teka Wondimu , Tabak Joël , Vo Theodore , Wechselberger Martin , Bertram Richard
Published: November 2011

Pituitary cells of the anterior pituitary gland secrete hormones in response to patterns of electrical activity. Several types of pituitary cells produce short bursts of electrical activity which are more effective than single spikes in evoking hormone release. These bursts, called pseudo-plateau bursts, are unlike bursts studied mathematically in neurons (plateau bursting) and the standard fast-slow analysis used for plateau bursting is of limited use. Using an alternative fast-slow analysis, with one fast and two slow variables, we show that pseudo-plateau bursting is a canard-induced mixed mode oscillation. Using this technique, it is possible to determine the region of parameter space where bursting occurs as well as salient properties of the burst such as the number of spikes in the burst. The information gained from this one-fast/two-slow decomposition complements the information obtained from a two-fast/one-slow decomposition.
Evaluation of genomic high-throughput sequencing data generated on Illumina HiSeq and Genome Analyzer systems
Authors: Minoche André E , Dohm Juliane C , Himmelbauer Heinz
Published: November 2011

Background

The generation and analysis of high-throughput sequencing data are becoming a major component of many studies in molecular biology and medical research. Illumina's Genome Analyzer (GA) and HiSeq instruments are currently the most widely used sequencing devices. Here, we comprehensively evaluate properties of genomic HiSeq and GAIIx data derived from two plant genomes and one virus, with read lengths of 95 to 150 bases.

Results

We provide quantifications and evidence for GC bias, error rates, error sequence context, effects of quality filtering, and the reliability of quality values. By combining different filtering criteria we reduced error rates 7-fold at the expense of discarding 12.5% of alignable bases. While overall error rates are low in HiSeq data we observed regions of accumulated wrong base calls. Only 3% of all error positions accounted for 24.7% of all substitution errors. Analyzing the forward and reverse strands separately revealed error rates of up to 18.7%. Insertions and deletions occurred at very low rates on average but increased to up to 2% in homopolymers. A positive correlation between read coverage and GC content was found depending on the GC content range.

Conclusions

The errors and biases we report have implications for the use and the interpretation of Illumina sequencing data. GAIIx and HiSeq data sets show slightly different error profiles. Quality filtering is essential to minimize downstream analysis artifacts. Supporting previous recommendations, the strand-specificity provides a criterion to distinguish sequencing errors from low abundance polymorphisms.
Validation Study of a Quantitative Multigene Reverse Transcriptase–Polymerase Chain Reaction Assay for Assessment of Recurrence Risk in Patients With Stage II Colon Cancer
Authors: Gray Richard G. , Quirke Philip , Handley Kelly , Lopatin Margarita , Magill Laura , Baehner Frederick L. , Beaumont Claire , Clark-Langone Kim M. , Yoshizawa Carl N. , Lee Mark , Watson Drew , Shak Steven , Kerr David J.
Published: November 2011

Purpose

We developed quantitative gene expression assays to assess recurrence risk and benefits from chemotherapy in patients with stage II colon cancer.

Patients and Methods

We sought validation by using RNA extracted from fixed paraffin-embedded primary colon tumor blocks from 1,436 patients with stage II colon cancer in the QUASAR (Quick and Simple and Reliable) study of adjuvant fluoropyrimidine chemotherapy versus surgery alone. A recurrence score (RS) and a treatment score (TS) were calculated from gene expression levels of 13 cancer-related genes (n = 7 recurrence genes and n = 6 treatment benefit genes) and from five reference genes with prespecified algorithms. Cox proportional hazards regression models and log-rank methods were used to analyze the relationship between the RS and risk of recurrence in patients treated with surgery alone and between TS and benefits of chemotherapy.

Results

Risk of recurrence was significantly associated with RS (hazard ratio [HR] per interquartile range, 1.38; 95% CI, 1.11 to 1.74; P = .004). Recurrence risks at 3 years were 12%, 18%, and 22% for predefined low, intermediate, and high recurrence risk groups, respectively. T stage (HR, 1.94; P < .001) and mismatch repair (MMR) status (HR, 0.31; P < .001) were the strongest histopathologic prognostic factors. The continuous RS was associated with risk of recurrence (P = .006) beyond these and other covariates. There was no trend for increased benefit from chemotherapy at higher TS (P = .95).

Conclusion

The continuous 12-gene RS has been validated in a prospective study for assessment of recurrence risk in patients with stage II colon cancer after surgery and provides prognostic value that complements T stage and MMR. The TS was not predictive of chemotherapy benefit.
Improvement in Breast Cancer Outcomes Over Time: Are Older Women Missing Out?
Authors: Smith Benjamin D. , Jiang Jing , McLaughlin Sandra S. , Hurria Arti , Smith Grace L. , Giordano Sharon H. , Buchholz Thomas A.
Published: November 2011

Purpose

Women aged ≥ 75 years account for 40,000 breast cancers/yr and are the most rapidly growing demographic. Recent data demonstrated that breast cancer death rates in the US population are declining, but it is not known whether death rates have declined similarly for older and younger women. We examined the following two outcomes: the rate of breast cancer death in the general population and the risk of breast cancer death in newly diagnosed patients, and we compared change over time in these outcomes for older versus younger women.

Methods

By using data from National Vital Statistics Reports that spanned from 1990 to 2007, the yearly change in the age-specific rate of breast cancer death was characterized with linear regression. With the use of the Surveillance, Epidemiology, and End Results nine-registry cohort that spanned from 1980 to 1997, the yearly change in age-specific risk of breast cancer death was characterized by using competing-risks regression adjusted for race and stage.

Results

Relative to 1990, the rate of breast cancer death in the general population decreased by 2.5%/yr for women age 20 to 49 years, 2.1%/yr for women age 50 to 64 years, 2.0%/yr for women age 65 to 74 years, and 1.1%/yr for women age ≥ 75 years. From 1980 to 1997, the adjusted risk of breast cancer death in newly diagnosed patients decreased by 3.6%/yr for women age less than 75 years versus 1.3%/yr for women age ≥ 75 years (P < .001). Over this time interval, the 10-year absolute risk of breast cancer death decreased by 15.3% for women age 50 to 64 years (from 31.9% to 16.6%) but by only 7.5% (from 24.8% to 17.4%) for women age ≥ 75 years.

Conclusion

Breast cancer outcomes have preferentially improved in women age less than 75 years. Focused research is needed to improve outcomes in older women.
RAS Mutations Are Associated With the Development of Cutaneous Squamous Cell Tumors in Patients Treated With RAF Inhibitors
Authors: Oberholzer Patrick A. , Kee Damien , Dziunycz Piotr , Sucker Antje , Kamsukom Nyam , Jones Robert , Roden Christine , Chalk Clinton J. , Ardlie Kristin , Palescandolo Emanuele , Piris Adriano , MacConaill Laura E. , Robert Caroline , Hofbauer Günther F.L. , McArthur Grant A. , Schadendorf Dirk , Garraway Levi A.
Published: November 2011

Purpose

RAF inhibitors are effective against melanomas with BRAF V600E mutations but may induce keratoacanthomas (KAs) and cutaneous squamous cell carcinomas (cSCCs). The potential of these agents to promote secondary malignancies is concerning. We analyzed cSCC and KA lesions for genetic mutations in an attempt to identify an underlying mechanism for their formation.

Methods

Four international centers contributed 237 KA or cSCC tumor samples from patients receiving an RAF inhibitor (either vemurafenib or sorafenib; n = 19) or immunosuppression therapy (n = 53) or tumors that developed spontaneously (n = 165). Each sample was profiled for 396 known somatic mutations across 33 cancer-related genes by using a mass spectrometric–based genotyping platform.

Results

Mutations were detected in 16% of tumors (38 of 237), with five tumors harboring two mutations. Mutations in TP53, CDKN2A, HRAS, KRAS, and PIK3CA were previously described in squamous cell tumors. Mutations in MYC, FGFR3, and VHL were identified for the first time. A higher frequency of activating RAS mutations was found in tumors from patients treated with an RAF inhibitor versus populations treated with a non–RAF inhibitor (21.1% v 3.2%; P < .01), although overall mutation rates between treatment groups were similar (RAF inhibitor, 21.1%; immunosuppression, 18.9%; and spontaneous, 17.6%; P = not significant). Tumor histology (KA v cSCC), tumor site (head and neck v other), patient age (≤ 70 v > 70 years), and sex had no significant impact on mutation rate or type.

Conclusion

Squamous cell tumors from patients treated with an RAF inhibitor have a distinct mutational profile that supports a mechanism of therapy-induced tumorigenesis in RAS-primed cells. Conceivably, cotargeting of MEK together with RAF may reduce or prevent formation of these tumors.