bluuebunny/crossref_metadata_2025 huggingface dataset:
TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype
Authors: Karanovic Una , Lei Lei , Martens Craig A , Barbian Kent , McClarty Grant , Caldwell Harlan D , Yang Chunfu
Published: October 2023

Chlamydia trachomatis is an obligate intracellular bacterium that causes blinding trachoma and sexually transmitted disease. The chlamydial plasmid is a critical virulence factor in the pathogenesis of these diseases. Plasmid gene protein 4 (Pgp4) plays a major role in chlamydial virulence by regulating the expression of both chromosomal genes and Pgp3. Despite the importance of Pgp4 in mediating lytic exit from host cells the pathogenic mechanism by which it functions is unknown. CT084 is a highly conserved chromosomal gene with homology to phospholipase D. We showed CT084 expression is regulated by Pgp4 and expressed late in the chlamydial developmental cycle. To investigate the function of CT084 in chlamydial lytic exit from infected cells, we made a CT084 null strain (ct084::bla) by using Targetron. The ct084::bla strain grew normally in vitro compared to wild-type strain; however, the strain did not lyse infected cells and produced significantly less and smaller plaques. Collectively, our finding shows Pgp4-regulated CT084-mediated chlamydia lytic exit from infected host cells.
Breathing and Oxygen Carrying Capacity in Ts65Dn and Down Syndrome
Authors: DeRuisseau Lara R , Receno Candace N , Cunningham Caitlin , Bates Melissa L , Goodell Morgan , Liang Chen , Eassa Brianna , Pascolla Jessica , DeRuisseau Keith C
Published: October 2023

Individuals with Down syndrome (Ds) are at increased risk of respiratory infection, aspiration pneumonia, and apnea. The Ts65Dn mouse is a commonly used model of Ds, but there have been no formal investigations of awake breathing and respiratory muscle function in these mice. We hypothesized that breathing would be impaired in Ts65Dn vs. wild-type (WT), and would be mediated by both neural and muscular inputs. Baseline minute ventilation was not different at 3, 6, or 12 mo of age. However, VT/Ti, a marker of the neural drive to breathe, was lower in Ts65Dn vs. WT and central apneas were more prevalent. The response to breathing hypoxia was not different, but the response to hypercapnia was attenuated, revealing a difference in carbon dioxide sensing, and/or motor output in Ts65Dn. Oxygen desaturations were present in room air, demonstrating that ventilation may not be sufficient to maintain adequate oxygen saturation in Ts65Dn. We observed no differences in arterial PO2 or PCO2, but Ts65Dn had lower hemoglobin and hematocrit. A retrospective medical record review of 52,346 Ds and 52,346 controls confirmed an elevated relative risk of anemia in Ds. We also performed eupneic in-vivo electromyography and in-vitro muscle function and histological fiber typing of the diaphragm, and found no difference between strains. Overall, conscious respiration is impaired in Ts65Dn, is mediated by neural mechanisms, and results in reduced hemoglobin saturation. Oxygen carrying capacity is reduced in Ts65Dn vs. WT, and we demonstrate that individuals with Ds are also at increased risk of anemia.
Adhesion and Polarity protein distribution-regulates hexagon dominated plasma membrane organization in Drosophila blastoderm embryos
Authors: Dey Bipasha , Mitra Debasmita , Das Tirthasree , Sherlekar Aparna , Balaji Ramya , Rikhy Richa
Published: October 2023

Epithelial cells contain polarity complexes on the lateral membrane and are organized in a hexagon-dominated polygonal array. The mechanisms regulating the organization of polygonal architecture in metazoan embryogenesis are not completely understood. Drosophila embryogenesis enables mechanistic analysis of epithelial polarity formation and its impact on polygonal organization. The plasma membrane (PM) of syncytial Drosophila blastoderm embryos is organized as a polygonal array with pseudocleavage furrow formation during the almost synchronous cortical division cycles. We find that polygonal (PM) organization arises in the metaphase (MP) of division cycle 11, and hexagon dominance occurs with an increase in furrow length in the metaphase of cycle 12. There is a decrease in cell shape index in metaphase from cycles 11 to 13. This coincides with Drosophila E-cad (DE-cadherin) and Bazooka enrichment at the edges and the septin, Peanut at the vertices of the furrow. We further assess the role of polarity and adhesion proteins in pseudocleavage furrow formation and its organization as a polygonal array. We find that DE-cadherin depletion leads to decreased furrow length, loss of hexagon dominance, and increased cell shape index. Bazooka and Peanut depletion lead to decreased furrow length, delay in onset of hexagon dominance from cycle 12 to 13, and increased cell shape index. Hexagon dominance occurs with an increase in furrow length in cycle 13 and increased DE-cadherin, possibly due to the inhibition of endocytosis. We conclude that polarity protein recruitment and regulation of endocytic pathways enable pseudocleavage furrow stability and the formation of a hexagon-dominated polygon array.
Recruiting a Person-Centered Direct Care Workforce Through Situational Judgement Tests: A Pilot Study in the Community Support of Older People in England
Authors: Wilberforce Mark , Dunn Abby , Tiffin Paul A
Published: October 2023

Background and Objectives

Studies have found that older people value care workers’ character traits and interpersonal judgment even more highly than their technical skills. Yet identifying these traits at recruitment is challenging. This study aimed to evaluate the first situational judgement tests (STJs) for direct care workers.

Research Design and Methods

Online tests were conducted with 251 care workers and members of the public in England. Participants evaluated the appropriateness of 61 potential behavioral responses to 11 “critical incidents,” each depicting challenging care work scenarios. Data collection included a measure of personality traits. A subsample of 72 participants completed a second “test-retest” assessment.

Results

A majority of test-takers (53%) found the test easy/very easy to complete, and most (55%) participants who worked in care reported the scenarios were highly realistic. Psychometric tests were positive. Test scores were unidimensional under a Confirmatory Factor Analysis (RMSEA = 0.038), and items broadly fit Rasch assumptions. Test–retest reliability (rho = 0.77) was acceptable, and for the general public sample, a modest increase in perceptions of the social standing of care work was observed. Test scores were positively correlated with 2 personality traits: agreeableness (r = 0.250, p < 0.001) and openness to experience (r = 0.179, p = .005). Test scores were not related to age, gender, or education level.

Discussion and Implications

The findings indicate support for the use of SJTs in direct care work. Its psychometric properties appear satisfactory, and collectively give confidence in the use of SJTs for assessing the suitability of candidates during recruitment. Further research should corroborate these findings in a new sample, and examine the relationship between test scores and job performance.
From strain to displacement: using deformation to enhance distributed acoustic sensing applications
Authors: Trabattoni Alister , Biagioli Francesco , Strumia Claudio , van den Ende Martijn , Scotto di Uccio Francesco , Festa Gaetano , Rivet Diane , Sladen Anthony , Ampuero Jean Paul , Métaxian Jean-Philippe , Stutzmann Éléonore
Published: October 2023

Over a period of less than a decade, distributed acoustic sensing (DAS) has become a well-established technology in seismology. For historical and practical reasons, DAS manufacturers usually provide instruments that natively record strain (rate) as the principal measurement. While at first glance strain recordings seem related to ground motion waveforms (displacement, velocity and acceleration), not all the seismological tools developed over the past century (e.g. magnitude estimation, seismic beamforming, etc.) can be readily applied to strain data. Notably, the directional sensitivity of DAS is more limited than conventional particle motion sensors, and DAS experiences an increased sensitivity to slow waves, often highly scattered by the subsurface structure and challenging to analyse. To address these issues, several strategies have been already proposed to convert strain rate measurements to particle motion. In this study, we focus on strategies based on a quantity we refer to as ‘deformation’. Deformation is defined as the change in length of the cable and is closely related to displacement, yet both quantities differ from one another: deformation is a relative displacement measurement along a curvilinear path. We show that if the geometry of the DAS deployment is made of sufficiently long rectilinear sections, deformation can be used to recover the displacement without the need of additional instruments. We validate this theoretical result using full-waveform simulations and by comparing, on a real data set, the seismic velocity recovered from DAS with that recorded by collocated seismometers. The limitations of this approach are discussed, and two applications are shown: enhancing direct P-wave arrivals and simplifying the magnitude estimation of seismic events. Converted displacement provides better sensitivity to high velocity phases, improves broadside response and permits the direct application of conventional seismological tools that are less effective when applied to strain (rate) data.
Inferring damage state and evolution with increasing stress using direct and coda wave velocity measurements in faulted and intact granite samples
Authors: Pandey Kiran , Taira Taka’aki , Dresen Georg , Goebel Thomas H
Published: October 2023

A better understanding of damage accumulation before dynamic failure events in geological material is essential to improve seismic hazard assessment. Previous research has demonstrated the sensitivity of seismic velocities to variations in crack geometry, with established evidence indicating that initial crack closure induces rapid changes in velocity. Our study extends these findings by investigating velocity changes by applying coda wave interferometry (CWI). We use an array of 16 piezoceramic transducers to send and record ultrasonic pulses and to determine changes in seismic velocity on intact and faulted Westerly granite samples. Velocity changes are determined from CWI and direct phase arrivals. This study consists of three sets of experiments designed to characterize variations in seismic velocity under various initial and boundary conditions. The first set of experiments tracks velocity changes during hydrostatic compression from 2 and 191 MPa in intact Westerly granite samples. The second set of experiments focuses on saw-cut samples with different roughness and examines the effects of confining pressure increase from 2 to 120 MPa. The dynamic formation of a fracture and the preceding damage accumulation is the focus of the third type of experiment, during which we fractured an initially intact rock sample by increasing the differential stress up to 780 MPa while keeping the sample confined at 75 MPa. The tests show that: (i) The velocity change for rough saw cut samples suggests that the changes in bulk material properties have a more pronounced influence than fault surface apertures or roughness. (ii) Seismic velocities demonstrate higher sensitivity to damage accumulation under increasing differential stress than macroscopic measurements. Axial stress measured by an external load cell deviates from linearity around two-third through the experiment at a stress level of 290 MPa higher than during the initial drop in seismic velocities. (iii) Direct waves exhibit strong anisotropy with increasing differential stress and accumulating damage before rock fracture. Coda waves, on the other hand, effectively average over elastic wave propagation for both fast and slow directions, and the resulting velocity estimates show little evidence for anisotropy. The results demonstrate the sensitivity of seismic velocity to damage evolution at various boundary conditions and progressive microcrack generation with long lead times before dynamic fracture.
Excess mortality in US Veterans during the COVID-19 pandemic: an individual-level cohort study
Authors: Weinberger Daniel M , Bhaskaran Krishnan , Korves Caroline , Lucas Brian P , Columbo Jesse A , Vashi Anita , Davies Louise , Justice Amy C , Rentsch Christopher T
Published: October 2023

Background

Most analyses of excess mortality during the COVID-19 pandemic have employed aggregate data. Individual-level data from the largest integrated healthcare system in the US may enhance understanding of excess mortality.

Methods

We performed an observational cohort study following patients receiving care from the Department of Veterans Affairs (VA) between 1 March 2018 and 28 February 2022. We estimated excess mortality on an absolute scale (i.e. excess mortality rates, number of excess deaths) and a relative scale by measuring the hazard ratio (HR) for mortality comparing pandemic and pre-pandemic periods, overall and within demographic and clinical subgroups. Comorbidity burden and frailty were measured using the Charlson Comorbidity Index and Veterans Aging Cohort Study Index, respectively.

Results

Of 5 905 747 patients, the median age was 65.8 years and 91% were men. Overall, the excess mortality rate was 10.0 deaths/1000 person-years (PY), with a total of 103 164 excess deaths and pandemic HR of 1.25 (95% CI 1.25–1.26). Excess mortality rates were highest among the most frail patients (52.0/1000 PY) and those with the highest comorbidity burden (16.3/1000 PY). However, the largest relative mortality increases were observed among the least frail (HR 1.31, 95% CI 1.30–1.32) and those with the lowest comorbidity burden (HR 1.44, 95% CI 1.43–1.46).

Conclusions

Individual-level data offered crucial clinical and operational insights into US excess mortality patterns during the COVID-19 pandemic. Notable differences emerged among clinical risk groups, emphasizing the need for reporting excess mortality in both absolute and relative terms to inform resource allocation in future outbreaks.
Development and investigation of a pollutants emission reduction process from a coal-gasification power plant integrated with fuel cell and solar energy
Authors: Yanto Doni Tri Putra , Akhmadeev Ravil , Hamad Hassan Salman , Alawadi Ahmed Hussien Radie , Abdullayev Abror Bozarboyevich , Romero-Parra Rosario Mireya , Fooladi Hadi
Published: October 2023

Even though coal resources are the most abundant among fossil fuels, coal-fired plants release large amounts of greenhouse gases into the atmosphere. In this regard, reducing environmental challenges and crises caused by coal burning can be a promising option to reduce today's crises in the energy field. The integration of coal-fired plants with renewable-driven energy systems can simultaneously improve thermodynamic performance and reduce pollutants emission rates. This article presents the thermodynamic and pollutant emission investigations of a new coal-fired plant coupled with a linear Fresnel solar collector (LFSC)-driven solar unit, a parabolic trough solar collector (PTSC)-driven solar unit, a high-temperature fuel cell stack (molten carbonate fuel cell stack [MCFCS]) and a heat recovery system (based on the steam turbine and gas turbine-based power cycles). The plant is able to produce electricity and hot water (HW). The main structure of the offered plant is based on coal, whereas, is coupled with renewables-based cycles to mitigate environmental impacts. The plant could generate ~ 207 MW of power and 3728 m3/h of HW. In such conditions, the energy efficiency of 73.1% and exergy efficiency of 44.18% could be achievable. Further, the emitted gas rates of the plant were nearly 403 tons/h. A comprehensive comparison is also presented for the plant's behavior under different types of coal (petcoke and anthracite). In addition, a two-function optimization is developed to determine the maximum value of exergy efficiency and the minimum value of total pollutants emission rate.
Enhancing Community Participation in Dengue Control Through Digital Crowdsourcing: An Analysis of a World Mosquito Program Digital Open Call in Sri Lanka
Authors: Sri-Pathmanathan Clarisse , Bao Huanyu , Diluka P A Eshani , Mee Alan , Andari Bekti , Saunders Eleanor , Wijegunawardana Asha , Weerasinghe Indika , Hetti Nimalka Pannila , Samaraweera Sudath , Tang Weiming , Tucker Joseph D
Published: October 2023

Background

Two crowdsourcing open calls were created to enhance community engagement in dengue control in Sri Lanka. We analyzed the process and outcomes of these digital crowdsourcing open calls.

Methods

We used standard World Health Organization methods to organize the open calls, which used exclusively digital methods because of coronavirus disease 2019 (COVID-19). We collected and analyzed sociodemographic information and digital engagement metrics from each submission. Submissions in the form of textual data describing community-led strategies for mosquito release were coded using grounded theory.

Results

The open calls received 73 submissions. Most people who submitted ideas spoke English, lived in Sri Lanka, and were 18 to 34 years old. The total Facebook reach was initially limited (16 161 impressions), prompting expansion to a global campaign, which reached 346 810 impressions over 14 days. Diverse strategies for the distribution of Wolbachia-infected mosquito boxes were identified, including leveraging traditional festivals, schools, and community networks. Fifteen submissions (21%) suggested the use of digital tools for monitoring and evaluation, sharing instructions, or creating networks. Thirteen submissions (18%) focused on social and economic incentives to prompt community engagement and catalyze community-led distribution.

Conclusions

Our project demonstrates that digital crowdsourcing open calls are an effective way to solicit creative and innovative ideas in a resource-limited setting.
The occurrence and genomic characteristics of the blaIMI-1 carbapenemase-producing Enterobacter cloacae complex retrieved from natural water sources in central Thailand
Authors: Songsaeng Wipawee , Prapasarakul Nuvee , Wongsurawat Thidathip , Sirichokchatchawan Wandee
Published: October 2023

Aim

Carbapenem resistance among Enterobacteriaceae is a serious threat to humans worldwide. This study aims to evaluate the phenotypic and genotypic characterization of carbapenemase-producing Enterobacter cloacae complex (ECC) retrieved from water sources in the central part of Thailand.

Methods and results

Samples were collected from water bodies surrounding farms and communities in central Thailand. The species were identified by using MALDI-TOF MS. The minimum inhibitory concentration (MIC) and antibiotic susceptibility were determined. The carbapenemase-producing genes were detected by PCR and whole genome sequencing (WGS). ECC with chromosome-encoded blaIMI-1 carbapenemase were detected. These isolates were resistant to last-resort antibiotics such as carbapenems and colistin as well as penicillin. In addition, all blaIMI-1 genes isolated from this study were found to be associated with chromosomally integrated Xer-dependent integrative mobile elements (IMEXs).

Conclusion

These findings highlight the diversity and dissemination of carbapenemases-producing Enterobacterales in environmental sources. With the increasing detection of carbapenemase genes worldwide, we should be aware of the blaIMI-producing E. cloacae complex with a high resistance profile and the ability to mobilize within the environment.
Deficiency of macrophage-derived Dnase1L3 causes lupus-like phenotypes in mice
Authors: Engavale Minal , Hernandez Colton J , Infante Angelica , LeRoith Tanya , Radovan Elliott , Evans Lauryn , Villarreal Johanna , Reilly Christopher M , Sutton R Bryan , Keyel Peter A
Published: October 2023

Systemic lupus erythematosus (SLE) is an autoimmune disease caused by environmental factors and loss of key proteins, including the endonuclease Dnase1L3. Dnase1L3 absence causes pediatric-onset lupus in humans, while reduced activity occurs in adult-onset SLE. The amount of Dnase1L3 that prevents lupus remains unknown. To genetically reduce Dnase1L3 levels, we developed a mouse model lacking Dnase1L3 in macrophages (conditional knockout [cKO]). Serum Dnase1L3 levels were reduced 67%, though Dnase1 activity remained constant. Homogeneous and peripheral antinuclear antibodies were detected in the sera by immunofluorescence, consistent with anti-double-stranded DNA (anti-dsDNA) antibodies. Total immunoglobulin M, total immunoglobulin G, and anti-dsDNA antibody levels increased in cKO mice with age. The cKO mice developed anti-Dnase1L3 antibodies. In contrast to global Dnase1L3−/− mice, anti-dsDNA antibodies were not elevated early in life. The cKO mice had minimal kidney pathology. Therefore, we conclude that an intermediate reduction in serum Dnase1L3 causes mild lupus phenotypes, and macrophage-derived DnaselL3 helps limit lupus.
Intentions at the End of Life: Continuous Deep Sedation and France’s Claeys-Leonetti law
Authors: Farrelly-Jackson Steven
Published: October 2023

In 2016, France passed a major law that is unique in giving terminally ill and suffering patients the right to the controversial procedure of continuous deep sedation until death (CDS). In so doing, the law identifies CDS as a sui generis clinical practice, distinct from other forms of palliative sedation therapy, as well as from euthanasia. As such, it reconfigures the ethical debate over CDS in interesting ways. This paper addresses one aspect of this reconfiguration and its implications for the intentions at work in this complex time at the end of life. The concept of intention is often considered central to the ethics of end-of-life care, but its role is recognized to be problematic, with charges of elusiveness and ambiguity. I aim to show that consideration of the French law affords a new understanding of the intentionality of CDS, and that in addition to the obvious importance of this for clarifying the ethics of the practice, it may suggest new ways of addressing the wider problem of ambiguous clinical intentions at end of life.
Trends in new and persistent opioid use in older adults with and without cancer
Authors: Baum Laura Van Metre , KC Madhav , Soulos Pamela R , Jeffery Molly M , Ruddy Kathryn J , Lerro Catherine C , Lee Hana , Graham David J , Rivera Donna R , Leapman Michael S , Jairam Vikram , Dinan Michaela A , Gross Cary P , Park Henry S
Published: October 2023

Background

The impact of ongoing efforts to decrease opioid use on patients with cancer remains undefined. Our objective was to determine trends in new and additional opioid use in patients with and without cancer.

Methods

This retrospective cohort study used data from Surveillance, Epidemiology, and End Results program–Medicare for opioid-naive patients with solid tumor malignancies diagnosed from 2012 through 2017 and a random sample of patients without cancer. We identified 238 470 eligible patients with cancer and further focused on 4 clinical strata: patients without cancer, patients with metastatic cancer, patients with nonmetastatic cancer treated with surgery alone (“surgery alone”), and patients with nonmetastatic cancer treated with surgery plus chemotherapy or radiation therapy (“surgery+”). We identified new, early additional, and long-term additional opioid use and calculated the change in predicted probability of these outcomes from 2012 to 2017.

Results

New opioid use was higher in patients with cancer (46.4%) than in those without (6.9%) (P < .001). From 2012 to 2017, the predicted probability of new opioid use was more stable in the cancer strata (relative declines: 0.1% surgery alone; 2.4% surgery+; 8.8% metastatic cancer), than in the noncancer stratum (20.0%) (P < .001 for each cancer to noncancer comparison). Early additional use declined among surgery patients (‒14.9% and ‒17.5% for surgery alone and surgery+, respectively) but was stable among patients with metastatic disease (‒2.8%, P = .50).

Conclusions

Opioid prescribing declined over time at a slower rate in patients with cancer than in patients without cancer. Our study suggests important but tempered effects of the changing opioid climate on patients with cancer.
DSLA 2023: Facing the dangers of data illiteracy
Authors: Gascon Gregg M , Wallman Katherine K , Ravula Sridhar , Cappelleri Joseph , Lee Julia , Lum Kristian , Nagaraja Chaitra H , Paul Sudeshna , Roy Dooti , Wu Edward
Published: October 2023

The Data Science and Literacy Act (DSLA) of 2023 aims to boost data literacy and data science education across the USA. Gregg M. Gascon, Katherine K. Wallman, Sridhar Ravula, Joseph Cappelleri, Julia Lee, Kristian Lum, Chaitra H. Nagaraja, Sudeshna Paul, Dooti Roy and Edward Wu explain why this opportunity demands our attention
Measuring crime in place: Distinguishing between area victimisation and area offences
Authors: Brunton-Smith Ian , Cernat Alexandru , Buil-Gil David , Pina-Sànchez Jose
Published: October 2023

Crime data is essential to the running of a safe society. But are we measuring crime in the best way? Ian Brunton-Smith, Alexandru Cernat, David Buil-Gil and Jose Pina-Sánchez explore how the current system could be improved
Building your career in statistics
Authors: Mallinckrodt Craig
Published: October 2023

Being a successful statistician or data scientist is about more than technical acumen, or simply working harder than you already are. Craig Mallinckrodt explains why the non-statistical aspects of statistical jobs are vital to get right
Metaphor repositories: the case of the mental health metaphor dictionary
Authors: Coll-Florit Marta , Climent Salvador
Published: October 2023

In recent years, there has been an emergence of online metaphor repositories. The purpose of this article is 2-fold. First, we present a review and comparison of the existing online databases of conceptual metaphors, showing that although there are a good number of domain-independent conceptual metaphor repositories based on English texts, repositories that are field-specific and/or in other languages are still scarce. Accordingly, the second goal of this article is to present the first metaphor repository specific to the mental health field, named The Mental Health Metaphor Dictionary. This repository is based on a Spanish corpus of first-person accounts published on social media (blogs and Twitter) by people suffering from severe mental disorders. We present the structure and the building process of the repository, and more significantly, we demonstrate its usefulness for a wide range of groups: professionals working in the field of mental health, public health communicators, family members or friends of people diagnosed with a mental disorder, the affected people themselves, and researchers of conceptual metaphors and discourse analysis in mental health.
Magnetic resonance imaging screening for osteonecrosis of the femoral head after coronavirus disease 2019
Authors: Takashima Kazuma , Iwasa Makoto , Ando Wataru , Uemura Keisuke , Hamada Hidetoshi , Mae Hirokazu , Maeda Yuki , Sugano Nobuhiko
Published: October 2023

Objectives

Systemic steroid administration has been suggested for the treatment of coronavirus disease 2019 (COVID-19), but the occurrence of osteonecrosis of the femoral head (ONFH) was one of the concerns for this treatment. This study aimed to use magnetic resonance imaging (MRI) to assess the incidence of ONFH after the treatment for COVID-19.

Methods

The study included 41 patients who were hospitalized and treated for pneumonia or other COVID-19-induced diseases. We conducted interviews with these patients regarding hip pain and performed MRI screenings for ONFH. The incidence and timing of ONFH after COVID-19 treatment were investigated.

Results

Of the 41 patients, one died of pneumonia and the remaining patients did not complain of hip pain. MRI screening was performed for 26 patients, and asymptomatic ONFH was detected in one patient (3.8%) whose ONFH appeared 1 month after the COVID-19 infection.

Conclusions

Our MRI screening of ONFH in post-COVID-19 patients revealed asymptomatic ONFH, which would not have been identified without active screening. Physicians should be aware that ONFH may occur in patients after treating COVID-19.
Overlapping systemic lupus erythematosus, seropositive erosive rheumatoid arthritis, and ANCA-associated vasculitis: A case report and literature review
Authors: Reesor Emma , Bhavsar Sankalp Virendrakumar
Published: October 2023

The overlap of rheumatoid arthritis and systemic lupus erythematosus is well described, with a syndrome known as ‘rhupus’. ANCA-associated vasculitis, however, is uncommonly associated with other autoimmune conditions. Here, we present a case of lupus, rheumatoid arthritis, and ANCA-associated vasculitis, with significant time elapsed between presentations, resulting in an accumulation of the three diagnoses over the course of 35 years. The patient was diagnosed with lupus at age 45, presenting with inflammatory polyarthritis, thrombocytopenia, leukopenia, positive ANA, positive anti-DNA, and hypocomplementemia. She was maintained on hydroxychloroquine therapy with minimal disease activity. Approximately 20 years later, she had a flare of polyarthritis with bilateral wrist erosive arthropathy and a positive rheumatoid factor, diagnosed as rheumatoid arthritis. Anti-TNFα therapy was initiated, and she was stable for a further 10 years. At age 79, she developed ANCA-associated vasculitis AAV with pulmonary and renal manifestations, treated with rituximab induction therapy and steroids. She recovered and her MPO-ANCA titre normalised. One year later, off the anti-TNFα, she again experienced an acute kidney injury with a repeat rise in MPO-ANCA. She was re-induced and maintained on steroids and rituximab. This novel case highlights the range of possible overlap syndromes, as well as how multiple autoimmune diagnoses can evolve over decades in previously stable disease. There is growing work around polyautoimmunity with ANCA-associated vasculitis and other systemic autoimmune diseases, which has potential to identify common aetiologies and pathogenesis. Knowledge of these overlap syndromes can help to recognise and manage these conditions in a timely manner.
Effects of salt and protein intake on polyuria in V2RA-treated ADPKD patients
Authors: Geertsema Paul , Koorevaar Iris W , Ipema Karin J R , Kramers Bart J , Casteleijn Niek F , Gansevoort Ron T , Meijer Esther
Published: October 2023

Background

The only treatment proven to be renoprotective in autosomal dominant polycystic kidney disease (ADPKD) is a vasopressin V2-receptor antagonist (V2RA). However, aquaresis-associated side effects limit tolerability. We investigated whether salt and/or protein intake influences urine volume and related endpoints in V2RA-treated ADPKD patients.

Methods

In this randomized, controlled, double-blind, crossover trial, ADPKD patients treated with maximally tolerated dose of a V2RA were included. While on a low salt and low protein diet, patients were given additional salt and protein to mimic regular intake, which was subsequently replaced by placebo in random order during four 2-week periods. Primary endpoint was change in 24-h urine volume. Secondary endpoints were change in quality of life, measured glomerular filtration rate (mGFR), blood pressure and copeptin level.

Results

Twelve patients (49 ± 8 years, 25.0% male) were included. Baseline salt and protein intake were 10.8 ± 1.3 g/24-h and 1.2 ± 0.2 g/kg/24-h, respectively. During the low salt and low protein treatment periods, intake decreased to 5.8 ± 1.6 g/24-h and 0.8 ± 0.1 g/kg/24-h, respectively. Baseline 24-h urine volume (5.9 ± 1.2 L) decreased to 5.2 ± 1.1 L (–11%, P = .004) on low salt and low protein, and to 5.4 ± 0.9 L (–8%, P = .04) on low salt. Reduction in 24-h urine volume was two times greater in patients with lower urine osmolality (–16% vs –7%). Polyuria quality of life scores improved in concordance with changes in urine volume. mGFR decreased during the low salt and low protein, while mean arterial pressure did not change during study periods. Plasma copeptin decreased significantly during low salt and low protein periods.

Conclusion

Lowering dietary salt and protein intake has a minor effect on urine volume in V2RA-treated ADPKD patients. Reduced intake of osmoles decreased copeptin concentrations and might thus increase the renoprotective effect of a V2RA in ADPKD patients.
Twenty-four-hour urine oxalate and risk of chronic kidney disease
Authors: Puurunen Marja , Kurtz Caroline , Wheeler Alistair , Mulder Kyra , Wood Kyle , Swenson Anna , Curhan Gary
Published: October 2023

Background

To assess whether 24-h urine oxalate (UOx) excretion is a risk factor for incident chronic kidney disease (CKD).

Methods

This longitudinal observational USA-based study included 426 896 individuals aged ≥18 years with no CKD at baseline and with at least one UOx, and at least 6 months of baseline and 6 months of follow-up data. Of these, 11 239 (2.6%) had an underlying malabsorptive condition. Incident CKD, defined by relevant International Classification of Diseases codes, was identified from a multi-source data cloud containing individual-level healthcare claims and electronic medical records data. The association between categories of UOx and incident CKD was modeled using logistic regression adjusting for age, sex, race, body mass index, baseline urine calcium, urine citrate, urine volume, tobacco use, hypertension, diabetes, malabsorption and cardiovascular disease.

Results

Mean follow-up time was 38.9 months (standard deviation 21.7). Compared with individuals with UOx <20 mg/24 h, the odds of developing incident CKD increased for UOx 20–29 mg/24 h [multivariable-adjusted odds ratio (MVOR) 1.14 (95% CI 1.07, 1.21)] through 80+ mg/24 h [MVOR 1.35 (1.21, 1.50)] and was statistically significant for each UOx category. A similar pattern was seen in the subgroup with a malabsorptive condition though the magnitudes of association were larger, with the odds of developing incident CKD increased for UOx 20–29 mg/24 h [MVOR 1.50 (1.03, 2.20)] through 80+ mg/24 h [MVOR 2.34 (1.50, 3.63)] as compared with UOx <20 mg/24 h.

Conclusions

The risk of incident CKD increases with increasing 24-h UOx excretion. Future studies should examine whether reducing UOx diminishes the risk of developing CKD.
The serine–glycine–one-carbon metabolic network orchestrates changes in nitrogen and sulfur metabolism and shapes plant development
Authors: Rosa-Téllez Sara , Alcántara-Enguídanos Andrea , Martínez-Seidel Federico , Casatejada-Anchel Ruben , Saeheng Sompop , Bailes Clayton L , Erban Alexander , Barbosa-Medeiros David , Alepúz Paula , Matus José Tomás , Kopka Joachim , Muñoz-Bertomeu Jesús , Krueger Stephan , Roje Sanja , Fernie Alisdair R , Ros Roc
Published: October 2023

L-serine (Ser) and L-glycine (Gly) are critically important for the overall functioning of primary metabolism. We investigated the interaction of the phosphorylated pathway of Ser biosynthesis (PPSB) with the photorespiration-associated glycolate pathway of Ser biosynthesis (GPSB) using Arabidopsis thaliana PPSB-deficient lines, GPSB-deficient mutants, and crosses of PPSB with GPSB mutants. PPSB-deficient lines mainly showed retarded primary root growth. Mutation of the photorespiratory enzyme Ser-hydroxymethyltransferase 1 (SHMT1) in a PPSB-deficient background resumed primary root growth and induced a change in the plant metabolic pattern between roots and shoots. Grafting experiments demonstrated that metabolic changes in shoots were responsible for the changes in double mutant development. PPSB disruption led to a reduction in nitrogen (N) and sulfur (S) contents in shoots and a general transcriptional response to nutrient deficiency. Disruption of SHMT1 boosted the Gly flux out of the photorespiratory cycle, which increased the levels of the one-carbon (1C) metabolite 5,10-methylene-tetrahydrofolate and S-adenosylmethionine. Furthermore, disrupting SHMT1 reverted the transcriptional response to N and S deprivation and increased N and S contents in shoots of PPSB-deficient lines. Our work provides genetic evidence of the biological relevance of the Ser–Gly–1C metabolic network in N and S metabolism and in interorgan metabolic homeostasis.
Sustainability trade-offs in animal husbandry: Consumers’ choice when they can't have it all
Authors: Klink-Lehmann Jeanette , Tatic Milan , Weingarten Nina , Hartmann Monika
Published: October 2023

This paper investigates consumer choices in the presence of conflicting goals, with and without information making conflicts salient. An experimental online study was conducted with a sample of German consumers, focusing on pig farming. The results show that personal health benefits outweigh animal welfare considerations, while the latter still weigh more than environment-related sustainability attributes. Providing information can have ambiguous effects, depending on the sustainability trade-off being investigated and the type of information provided. The findings reveal that consumers are heterogeneous in their preferences for different protected goods, and these differences can be attributed to psychographic and socio-demographic factors.
The Cost of Bank Regulatory Capital
Authors: Plosser Matthew C , Santos João A C
Published: October 2023

Basel I introduced capital requirements for undrawn commitments, but only for revolvers with an original maturity greater than one year. We use this regulatory discontinuity to estimate the impact of capital regulation on the cost and composition of credit. Following Basel I, short-term commitment fees declined relative to long-term commitments and issuance of short-term facilities increased. Our results highlight the sensitivity of credit provision to capital regulation, particularly for banks with less capital. We are able to infer that low-capital banks are willing to forego twice as much income from fees to reduce required regulatory capital by a dollar.
A Rule of Law Analysis: Botswana’s Non-Conviction-Based Confiscation and Forfeiture Regime Under the Proceeds and Instruments of Crime Act, 2014
Authors: Lekgowe Gosego Rockfall
Published: October 2023

On its inception, the non-conviction-based asset confiscation and forfeiture regime attracted both praise and criticism. Using the Rule of Law as an analytic framework, this paper evaluates the non-conviction-based asset confiscation and forfeiture regime under Botswana’s Proceeds and Instruments of Crime Act, 2014. The paper finds that whilst the regime has withstood constitutional attacks, it still retains some shortcomings. For instance, there is lack of clarity on the standards of proof, procedures and inadequate protection of third-party rights. The paper recommends reforms.
Weak response of bovine hepcidin induction to iron through decreased expression of Smad4
Authors: Sadakane Hiroyuki , Matsumura Manami , Murakami Masaru , Itoyama Erina , Shimokawa Fumie , Sakota Shotaro , Yoshioka Hidetugu , Kawabata Hiroshi , Matsui Tohru , Funaba Masayuki
Published: October 2023

Hepcidin negatively regulates systemic iron levels by inhibiting iron entry into the circulation. Hepcidin production is increased in response to an increase in systemic iron via the activation of the bone morphogenetic protein (BMP) pathway. Regulation of hepcidin expression by iron status has been proposed on the basis of evidence mainly from rodents and humans. We evaluated the effect of iron administration on plasma hepcidin concentrations in calves and the expression of bovine hepcidin by the BMP pathway in a cell culture study. Hematocrit as well as levels of blood hemoglobin and plasma iron were lower than the reference level in calves aged 1–4 weeks. Although intramuscular administration of iron increased iron‐related parameters, plasma hepcidin concentrations were unaffected. Treatment with BMP6 increased hepcidin expression in human liver‐derived cells but not in bovine liver‐derived cells. A luciferase‐based reporter assay revealed that Smad4 was required for hepcidin reporter transcription induced by Smad1. The reporter activity of hepcidin was lower in the cells transfected with bovine Smad4 than in those transfected with murine Smad4. The lower expression levels of bovine Smad4 were responsible for the lower activity of the hepcidin reporter, which might be due to the instability of bovine Smad4 mRNA. In fact, the endogenous Smad4 protein levels were lower in bovine cells than in human and murine cells. Smad4 also confers TGF‐β/activin‐mediated signaling. Induction of TGF‐β‐responsive genes was also lower after treatment with TGF‐β1 in bovine hepatocytes than in human hepatoma cells. We revealed the unique regulation of bovine hepcidin expression and the characteristic TGF‐β family signaling mediated by bovine Smad4. The present study suggests that knowledge of the regulatory expression of hepcidin as well as TGF‐β family signaling obtained in murine and human cells is not always applicable to bovine cells.
Targeting sphingosine kinase 1 in p53KO thymic lymphoma
Authors: Velazquez Fabiola N. , Stith Jeffrey L. , Zhang Leiqing , Allam Amira M. , Haley John , Obeid Lina M. , Snider Ashley J. , Hannun Yusuf A.
Published: October 2023

Sphingosine kinase 1 (SK1) is a key sphingolipid enzyme that is upregulated in several types of cancer, including lymphoma which is a heterogenous group of malignancies. Treatment for lymphoma has improved significantly by the introduction of new therapies; however, subtypes with tumor protein P53 (p53) mutations or deletion have poor prognosis, making it critical to explore new therapeutic strategies in this context. SK1 has been proposed as a therapeutic target in different types of cancer; however, the effect of targeting SK1 in cancers with p53 deletion has not been evaluated yet. Previous work from our group suggests that loss of SK1 is a key event in mediating the tumor suppressive effect of p53. Employing both genetic and pharmacological approaches to inhibit SK1 function in Trp53KO mice, we show that targeting SK1 decreases tumor growth of established p53KO thymic lymphoma. Inducible deletion of Sphk1 or its pharmacological inhibition drive increased cell death in tumors which is accompanied by selective accumulation of sphingosine levels. These results demonstrate the relevance of SK1 in the growth and maintenance of lymphoma in the absence of p53 function, positioning this enzyme as a potential therapeutic target for the treatment of tumors that lack functional p53.
Study of sustainable development goals of Syrian higher education: strategy, effects and future insights
Authors: Al-Raeei Marwan
Published: October 2023

Purpose

This research mainly aims to shed light on sustainable development in Syrian higher education during the Syrian crisis.

Design/methodology/approach

In this research, the authors mainly study sustainable development in the Syrian Arab Republic through the bibliometrics data of universities and research centres in Syrian Arab Republic, where these data are related to sustainable development. Also, the authors study the strategies used in the university with the largest research output of sustainable development in the Syrian Arab Republic. The authors extract research data arranged in sustainable development goals in the Syrian Arab Republic from SciVal database.

Findings

Based on the bibliometric data on sustainable development research in the Syrian Arab Republic, the authors find that universities and research centres in the Syrian Arab Republic have taken important steps towards the goals of sustainable development, especially in the year 2022. The authors also find that the University of Damascus had the largest share in research related to the goals of sustainable development, and the authors find that this is due to the strategies taken by the University of Damascus towards the goals of sustainable development, such as the policy of digital transformation, the policy of recycling and the increase of green areas in the university.

Originality/value

This study is the first of its kind to study the goals of sustainable development in Syrian higher education during the Syrian crisis period.
Job pride and work orientation among blue-collar workers in the Finnish vehicle industry
Authors: Koivunen Tuija , Pyöriä Pasi , Saari Tiina
Published: October 2023

Purpose

Although the number of blue-collar industrial workers has been declining, manufacturing jobs continue to have considerable importance, even in technologically advanced economies. This study gives a voice to this often-overlooked group of workers, focusing on the Finnish vehicle industry.

Design/methodology/approach

The study assessed how manual workers in automotive manufacturing describe their job pride and how their accounts were related to conceptualizations of work orientation. The data included semi-structured interviews and an open-ended survey question on situations in which the respondents had felt proud of their work. The data were analysed using thematic analysis.

Findings

According to the results, the respondents had a high level of job pride in general, but the meaning given to this attitude varied considerably, depending on the situation. The study participants' work orientation was a mixture of instrumental and intrinsic traits. However, there were also respondents who did not experience job pride or who had lost it because of the work circumstances.

Originality/value

Relatively little research has assessed the importance of job pride in the context of industrial manufacturing. Recognizing job pride in its variety is crucial information for employers who aim to develop working conditions and employee retention.
Family in societal redistribution: a theoretical inquiry
Authors: Frericks Patricia
Published: October 2023

Purpose

The family is one of the foundations of society; its significance for societal redistribution in modern societies, though, remains particularly unclear. A major reason for this is that theoretical approaches to societal redistribution have not adequately included family either in social philosophy or in welfare state theory. As a consequence, also empirical analyses of differences and developments in societal redistribution have not included family or only in as far as family is affected by other redistributive principles. This paper contributes to filling this theoretical gap.

Design/methodology/approach

This paper theorises family as a redistributive principle. With reference to the major theoretical concepts of redistribution, it identifies the relevant dimensions of family in societal redistribution and develops a typology of its inclusion in societal redistribution.

Findings

Approaches to redistribution are shaped by distinct concepts of equal or unequal exchange, the relevant actors they identify and by different understandings of the economy. These distinctions are central to understanding the position of family in societal redistribution. With reference to the major theoretical concepts of redistribution, this paper identifies the relevant dimensions of family in societal redistribution and develops a typology of its inclusion in societal redistribution. Further investigations might draw on this typology and detect the theoretical foundations of its conceptualisations and its similarities to and deviations from the developed types.

Originality/value

This paper provides a theoretical groundwork for theoretical and empirical investigations of societal redistribution and for better comprehending its international variation. It aims to initiate a fundamental rethinking of the usual understanding of societal redistribution that widely ignores family as a redistributive principle of its own.