bluuebunny/crossref_metadata_2025 huggingface dataset:
Trade Facilitation and Agriculture Sector Performance in Sub-Saharan Africa: Insightful Policy Implications for Economic Sustainability
Authors: Ibrahim Ridwan Lanre , Yu Zhang , Hassan Shafiqul , Ajide Kazeem Bello , Tanveer Muhammad , Khan Abdul Rehman
Published: July 2022

This study investigates the impact of trade facilitation though costs, documents, and time to import and export—on agricultural sector performance (ASP) for a panel of 33 Sub-Saharan African (SSA) countries from 2005 to 2019. The empirical analysis is based on a dynamic system of generalized method of moments. The following findings are established. First, higher import costs, documents, and time significantly improve ASP, while exports negatively impact ASP. Second, while import costs promote trade in agricultural products through substitution effects, export costs impede it. Third, among other factors, improvements in human capital, gross fixed capital formation, population growth, and trade openness significantly improve agricultural sector performance. Fourth, the results of the robustness check further emphasize the importance of TF on ASP from various dimensions. The study concludes that improving TF procedures by reducing costs, documents, and time in cross-border trade remains crucial to boosting agriculture sector performance in SSA.
The trans DNA cleavage activity of Cas12a provides no detectable immunity against plasmid or phage
Authors: Liu Shunhang , Rao Xichen , Zhao Ruiliang , Han Wenyuan
Published: July 2022

Cas12a is a type V-A CRISPR-Cas RNA-guided endonuclease. It cleaves dsDNA at specific site, and then is activated for nonspecific ssDNA cleavage in trans in vitro. The immune function of the trans activity is still unknown. To address this question, we constructed a Cas12a targeting system in Escherichia coli, where Cas12a cleaved a high-copy target plasmid to unleash the trans ssDNA cleavage activity. Then, we analyzed the effect of the Cas12a targeting on a non-target plasmid and a ssDNA phage. The results show that Cas12a efficiently eliminates target plasmid but exerts no impact on the maintenance of the non-target plasmid or plague formation efficiency of the phage. In addition, a two-spacer CRISPR array, which facilitates target plasmid depletion, still has no detectable effect on the non-target plasmid or phage either. Together, the data suggest that the trans ssDNA cleavage of Cas12a does not contribute to immunity in vivo.
An immune-related gene prognostic risk index for pancreatic adenocarcinoma
Authors: Su Yang , Qi Ruoshan , Li Lanying , Wang Xu , Li Sijin , Zhao Xuan , Hou Rui , Ma Wen , Liu Dan , Zheng Junnian , Shi Ming
Published: July 2022

Objective

Our goal is to construct an immune-related gene prognostic risk index (IRGPRI) for pancreatic adenocarcinoma (PAAD), and to clarify the immune and molecular features in IRGPRI-defined PAAD subgroups and the benefit of immune checkpoint inhibitors (ICIs) therapy.

Method

Through differential gene expression analysis, weighted gene co-expression network analysis (WGCNA), and univariate Cox regression analysis, 16 immune-related hub genes were identified using the Cancer Genome Atlas (TCGA) PAAD dataset (n = 182) and immune gene set. From these genes, we constructed an IRGPRI with the Cox regression method and the IRGPRI was verified based on the Gene Expression Omnibus (GEO) dataset (n = 45). Then, we analyzed the immune and molecular features and the benefit of ICI therapy in IRGPRI-defined subgroups.

Results

Five genes, including S100A16, CD40, VCAM1, TNFRSF4 and TRAF1 were used to construct IRGPRI. As with the results of the GEO cohort, the overall survival (OS) was more favorable in low IRGPRI patients versus high IRGPRI patients. The composite results pointed out that low IRGPRI was associated with immune response-related pathways, high level of CTLA4, low KRAS and TP53 mutation rate, more infiltration of activated memory CD4+ T cells, CD8+ T cells, and more benefits from ICIs therapy. In comparison, high IRGPRI was associated with cancer-related pathways, low expression of CTLA4, high KRAS and TP53 mutation rate, more infiltration of M2 macrophages, and less benefit from ICIs therapies.

Conclusion

This IRGPRI is an encouraging biomarker to define the prognosis, immune and molecular features, and benefits from ICIs treatments in PAAD.
Ferroptosis in inflammatory arthritis: A promising future
Authors: Chang Siyuan , Tang Mengshi , Zhang Bikui , Xiang Daxiong , Li Fen
Published: July 2022

Ferroptosis is a kind of regulatory cell death (RCD) caused by iron accumulation and lipid peroxidation, which is characterized by mitochondrial morphological changes and has a complex regulatory network. Ferroptosis has been gradually emphasized in the pathogenesis of inflammatory arthritis. In this review, we summarized the relevant research on ferroptosis in various inflammatory arthritis including rheumatoid arthritis (RA), osteoarthritis, gout arthritis, and ankylosing spondylitis, and focused on the relationship between RA and ferroptosis. In patients with RA and animal models of RA, there was evidence of iron overload and lipid peroxidation, as well as mitochondrial dysfunction that may be associated with ferroptosis. Ferroptosis inducers have shown good application prospects in tumor therapy, and some anti-rheumatic drugs such as methotrexate and sulfasalazine have been shown to have ferroptosis modulating effects. These phenomena suggest that the role of ferroptosis in the pathogenesis of inflammatory arthritis will be worth further study. The development of therapeutic strategies targeting ferroptosis for patients with inflammatory arthritis may be a promising future.
Elevated expression of odorant receptors and odorant-binding proteins genes detected in antennae of Culex quinquefasciatus field females
Authors: Santos Suzane Alves , Romão Tatiany Patricia , Helvecio Elisama , Barbosa Rosangela Maria Rodrigues , Leal Walter Soares , Ayres Constância Flávia Junqueira
Published: July 2022

Culex quinquefasciatus is responsible for the transmission of filarial worms and several arboviruses. Olfaction plays a crucial role in disease transmission as it influences behaviors that are essential for the survival and reproduction of the mosquito, such as the host-seeking behavior, courtship, and oviposition. Understanding the molecular events that coordinate how mosquitoes find their host may lead to alternative methods to reduce diseases transmission. Our aim was to investigate the differential expression profile of odorant receptor (ORs) and odorant-binding proteins (OBPs) genes in Cx. quinquefasciatus field females compared with CqSLab laboratory mosquito colony. Seventeen genes of interest were evaluated for their qualitative and specific expression by RT-PCR on RNAs extracted from female antennae, female legs, complete male bodies, incomplete female bodies (no head and no legs), and L4 larvae. The general expression mapping of olfactory genes revealed that all analyzed genes were expressed in antennae. Some genes showed different qualitative expression profiles, such as CquiOR2, CquiOR64, CquiOR93, CquiOBP11, and CquiOBP16, which were expressed exclusively in female antennae. On the other hand, CquiOR37, CquiOBP2, and CquiOBP43 are expressed in all sample types, and CquiOBP10 was expressed in female antennae and legs and in the complete male bodies. The expression of CquiOBP5 was detected in the female’s antennae and body, but it was absent in the legs. The quantitative differential expression analysis of six of the 17 genes by RT-qPCR was performed from RNA samples from antenna pools collected in three physiological states, post-emergence, post-mating, and post-blood feeding of the field females and CqSLab. A total of 3,600 antennae were analyzed, in pools containing 100 pairs. Most genes screened showed a higher expression level in field mosquitoes when compared with the laboratory strain CqSLab. The expression of CquiOBP5 and CquiOBP10 genes was significantly different between the post-mating and post blood-meal samples of laboratory females (p < 0.05). Our results suggest specialization of the function of the genes studied and divergence in the expression pattern of field mosquitoes compared with laboratory mosquitoes, and therefore, caution should be exercised in the interpretation of data from laboratory mosquito studies.
Evaluating carbon-electrode dielectrophoresis under the ASSURED criteria
Authors: Martinez-Duarte Rodrigo , Mager Dario , Korvink Jan G. , Islam Monsur
Published: July 2022

Extreme point-of-care refers to medical testing in unfavorable conditions characterized by a lack of primary resources or infrastructure. As witnessed in the recent past, considerable interest in developing devices and technologies exists for extreme point-of-care applications, for which the World Health Organization has introduced a set of encouraging and regulating guidelines. These are referred to as the ASSURED criteria, an acronym for Affordable (A), Sensitive (S), Specific (S), User friendly (U), Rapid and Robust (R), Equipment-free (E), and Delivered (D). However, the current extreme point of care devices may require an intermediate sample preparation step for performing complex biomedical analysis, including the diagnosis of rare-cell diseases and early-stage detection of sepsis. This article assesses the potential of carbon-electrode dielectrophoresis (CarbonDEP) for sample preparation competent in extreme point-of-care, following the ASSURED criteria. We first discuss the theory and utility of dielectrophoresis (DEP) and the advantages of using carbon microelectrodes for this purpose. We then critically review the literature relevant to the use of CarbonDEP for bioparticle manipulation under the scope of the ASSURED criteria. Lastly, we offer a perspective on the roadmap needed to strengthen the use of CarbonDEP in extreme point-of-care applications.
Distribution, pathogenicity and disease control of Fusarium tricinctum
Authors: Wang Yun , Wang Ruoyu , Sha Yuexia
Published: July 2022

Plant pathogenic fungi such as Fusarium tricinctum cause various plant diseases worldwide, especially in temperate regions. In cereals, F. tricinctum is one of the most common species causing Fusarium head blight (FHB) and root rot. Infection with F. tricinctum results in high yield losses and reduction in quality, mainly due to mycotoxin contamination of grain. Mycotoxins produced by F. tricinctum, such as enniatins (ENs) and moniliformin (MON), which are the most studied mycotoxins, have been reported to have multiple toxic effects on humans and animals. Although chemical control of Fusarium infection has been applied to grains, it is not always effective in controlling disease or reducing the level of mycotoxins in wheat grains. To the contrary, chemical control may significantly increase infection of F. tricinctum in fungicide-treated plots after treatment. Our studies show that the bacterium Bacillus amyloliquefaciens, has good control effects against F. tricinctum. Therefore, its use as a biological control agent against various plant pathogens may be an effective strategy to control the spread of Fusarium pathogens. Here, we conduct a review of the literature involving this plant pathogen, its diversity, virulence, and methods to control.
The Assessment on Synergistic Activity of Ebselen and Silver Ion Against Yersinia pseudotuberculosis
Authors: Dong Chuanjiang , Chen Wei , Zou Lili , Liu Binbin , Deng Kaihong , Guo Dingrui , Wang Peng , Chen Hao , Wang Helen , Wang Jun
Published: July 2022

Yersinia pseudotuberculosis is a foodborne zoonotic bacterium that is pathogenic to guinea pigs, rabbits, and mice. It also causes pseudotuberculosis in humans. However, it still lacked the scientific basis for control. Here, we found out that Ebselen (EbSe) exhibited synergistic antibacterial activity with silver nitrate (Ag+) against Y. pseudotuberculosis YpIII strain with high efficacy in vitro using UV-visible light absorption spectrum, 5,5’-dithiobis-(2-nitrobenzoic acid), laser scanning confocal microscope, flow cytometry, transmission electron microscopy and Western blotting assays. The depletion of total glutathione (GSH) amount and inhibition of thioredoxin reductase (TrxR) activity in thiol-dependent redox system revealed the destructiveness of EbSe-Ag+-caused intracellular oxidative stress. Furthermore, a YpIII-caused mice gastroenteritis model was constructed. EbSe-Ag+ significantly reduced bacterial loads with low toxicity. It also down-regulated the expression levels of interferon (IL)-1β and tumor necrosis factor-α, up-regulated the expression level of IL-10 on-site. All the in vivo results demonstrated the antibacterial activity and immune-modulatory property of EbSe-Ag+. Collectively, these results provided academic fundament for further analysis and development of EbSe-Ag+ as the antibacterial agents for pseudotuberculosis control.
Impaired fasting glucose, oxidative distress, and cognitive impairment. Is this the starting point on DBT cognitive decline?
Authors: Canal María Pilar , Nini Karen Agustina , Baez Maria Verónica
Published: July 2022

Different studies performed in human patients, animal models, and in vitro cell cultures, show a correlation between type 2 diabetes (DBT2) and certain neurodegenerative pathologies. Also, it was proposed that increased inflammation and- or oxidative distress are a possible cause of DBT2-accelerated cognitive decline. The onset of DBT2 is characterized by an increase in blood glucose levels due to (an inability of the body’s cells to use insulin properly) called impaired fasting glucose (IFG). Genetic and/or molecular causes of IFG have not yet been established, but metabolic syndrome, obesity, unbalanced diets, and sedentary lifestyle would be responsible, at least in part, for the multiplication in the number of this disease. It has been proposed that hyperglycemia itself causes an imbalance in the redox state and could compromise blood-brain barrier (BBB) causing neurodegeneration. For this reason, we propose, in this review, to evaluate the available data about redox state and neurocognitive studies during the IFG period.
Comparisons between cross-section and long-axis-section in the quantification of aneurysmal wall enhancement of fusiform intracranial aneurysms in identifying aneurysmal symptoms
Authors: Peng Fei , Liu Lang , Niu Hao , Feng Xin , Zhang Hong , He Xiaoxin , Xia Jiaxiang , Xu Boya , Bai Xiaoyan , Li Zhiye , Sui Binbin , Liu Aihua
Published: July 2022

Background

To investigate the quantification of aneurysmal wall enhancement (AWE) in fusiform intracranial aneurysms (FIAs) and to compare AWE parameters based on different sections of FIAs in identifying aneurysm symptoms.

Methods

Consecutive patients were prospectively recruited from February 2017 to November 2019. Aneurysm-related symptoms were defined as sentinel headache and oculomotor nerve palsy. All patients underwent high resolution magnetic resonance imaging (HR-MRI) protocol, including both pre and post-contrast imaging. CRstalk (signal intensity of FIAs' wall divided by pituitary infundibulum) was evaluated both in the cross-section (CRstalk−cross) and the long-axis section (CRstalk−long) of FIAs. Aneurysm characteristics include the maximal diameter of the cross-section (Dmax), the maximal length of the long-axis section (Lmax), location, type, and mural thrombus. The performance of parameters for differentiating symptomatic and asymptomatic FIAs was obtained and compared by a receiver operating characteristic (ROC) curve.

Results

Forty-three FIAs were found in 43 patients. Eighteen (41.9%) patients who presented with aneurysmal symptoms were classified in the symptomatic group. In univariate analysis, male sex (P = 0.133), age (P = 0.013), FIAs type (P = 0.167), mural thrombus (P = 0.130), Lmax (P = 0.066), CRstalk−cross (P = 0.027), and CRstalk−long (P = 0.055) tended to be associated with aneurysmal symptoms. In the cross-section model of multivariate analysis, male (P = 0.038), age (P = 0.018), and CRstalk−cross (P = 0.048) were independently associated with aneurysmal symptoms. In the long-axis section model of multivariate analysis, male (P = 0.040), age (P = 0.010), CRstalk−long (P = 0.046), and Lmax (P = 0.019) were independently associated with aneurysmal symptoms. In the combination model of multivariate analysis, male (P = 0.027), age (P = 0.011), CRstalk−cross (P = 0.030), and Lmax (P = 0.020) were independently associated with aneurysmal symptoms. CRstalk−cross has the highest accuracy in predicting aneurysmal symptoms (AUC = 0.701). The combination of CRstalk−cross and Lmax exhibited the highest performance in discriminating symptomatic from asymptomatic FIAs (AUC = 0.780).

Conclusion

Aneurysmal wall enhancement is associated with symptomatic FIAs. CRstalk−cross and Lmax were independent risk factors for aneurysmal symptoms. The combination of these two factors may improve the predictive performance of aneurysmal symptoms and may also help to stratify the instability of FIAs in future studies.
The altered intrinsic functional connectivity after acupuncture at shenmen (HT7) in acute sleep deprivation
Authors: Ning Yanzhe , Zheng Sisi , Feng Sitong , Yao Hao , Feng Zhengtian , Liu Xinzi , Dong Linrui , Jia Hongxiao
Published: July 2022

Introduction

Accumulating evidence has shown that acupuncture could significantly improve the sleep quality and cognitive function of individuals suffering from insufficient sleep. Numerous animal studies have confirmed the effects and mechanisms of acupuncture on acute sleep deprivation (SD). However, the role of acupuncture on individuals after acute SD remains unclear.

Methods

In the current study, we recruited 30 healthy subjects with regular sleep. All subjects received resting-state fMRI scans during the rested wakefulness (RW) state and after 24 h of total SD. The scan after 24 h of total SD included two resting-state fMRI sessions before and after needling at Shenmen (HT7). Both edge-based and large-scale network FCs were calculated.

Results

The edge-based results showed the suprathreshold edges with abnormal between-network FC involving all paired networks except somatosensory motor network (SMN)-SCN between the SD and RW state, while both decreased and increased between-network FC of edges involving all paired networks except frontoparietal network (FPN)-subcortical network (SCN) between before and after acupuncture at HT7. Compared with the RW state, the large-scale brain network results showed decreased between-network FC in SMN-Default Mode Network (DMN), SMN-FPN, and SMN-ventral attention network (VAN), and increased between-network FC in Dorsal Attention Network (DAN)-VAN, DAN-SMN between the RW state and after 24 h of total SD. After acupuncture at HT7, the large-scale brain network results showed decreased between-network FC in DAN-VAN and increased between-network FC in SMN-VAN.

Conclusion

Acupuncture could widely modulate extensive brain networks and reverse the specific between-network FC. The altered FC after acupuncture at HT7 may provide new evidence to interpret neuroimaging mechanisms of the acupuncture effect on acute SD.
Risk factors and prediction model of sleep disturbance in patients with maintenance hemodialysis: A single center study
Authors: Xu Rongpeng , Miao Liying , Ni Jiayuan , Ding Yuan , Song Yuwei , Yang Chun , Zhu Bin , Jiang Riyue
Published: July 2022

Objectives

This study aimed to explore the risk factors and develop a prediction model of sleep disturbance in maintenance hemodialysis (MHD) patients.

Methods

In this study, 193 MHD patients were enrolled and sleep quality was assessed by Pittsburgh Sleep Quality Index. Binary logistic regression analysis was used to explore the risk factors for sleep disturbance in MHD patients, including demographic, clinical and laboratory parameters, and that a prediction model was developed on the basis of risk factors by two-way stepwise regression. The final prediction model is displayed by nomogram and verified internally by bootstrap resampling procedure.

Results

The prevalence of sleep disturbance and severe sleep disturbance in MHD patients was 63.73 and 26.42%, respectively. Independent risk factors for sleep disturbance in MHD patients included higher 0.1*age (OR = 1.476, 95% CI: 1.103–1.975, P = 0.009), lower albumin (OR = 0.863, 95% CI: 0.771–0.965, P = 0.010), and lower 10*calcium levels (OR = 0.747, 95% CI: 0.615–0.907, P = 0.003). In addition, higher 0.1*age, lower albumin levels, and anxiety were independently associated with severe sleep disturbance in MHD patients. A risk prediction model of sleep disturbance in MHD patients showed that the concordance index after calibration is 0.736, and the calibration curve is approximately distributed along the reference line.

Conclusions

Older age, lower albumin and calcium levels are higher risk factors of sleep disturbance in MHD, and the prediction model for the assessment of sleep disturbance in MHD patients has excellent discrimination and calibration.
The global burden and temporal trend of cancer attributable to high body mass index: Estimates from the Global Burden of Disease Study 2019
Authors: Zhi Xing , Kuang Xiao-hong , Liu Kuan , Li Jian
Published: July 2022

Objective

The purpose of the study was to describe the burden and temporal trend of cancer attributable to high body mass index (BMI), with major patterns highlighted by sex, Socio-demographic Index (SDI), and geographical region.

Methods

This population-based observational study collected epidemiological data on cancer attributable to high BMI from the Global Burden of Diseases (GBD) 2019. The obtained data included deaths, disability-adjusted life-years (DALYs), and their age-standardized rates at the global, gender, SDI, regional, and country levels. The trend magnitudes and directions over time for mortality were analyzed. The associations between SDI and burden of cancer attributable to high BMI were also evaluated by Pearson correlation analysis.

Results

Worldwide, 462.55 thousand deaths and 11.18 million DALYs of cancer were related to high BMI in 2019, and both have more than doubled since 1990. An annual 0.6% increase was observed for the age-standardized mortality rate (ASMR), and the rate of increase slowed after 2000. In general, the burden of cancer attributable to high BMI was heavier in regions with higher SDI levels, whereas the increase slowed down or even showed a decreasing trend in the recent years. In contrast, in regions with lower SDI levels, although the baseline burden of cancer attributable to high BMI was relatively low, both the numbers and rates of deaths and DALYs showed a significantly increasing trend and may not stop increasing for a period of time. The trend and magnitude of high BMI-related cancer burden varied substantially in different anatomical sites. The leading three cancers of DALYs attributable to high BMI in 2019 were esophageal, colorectal, and liver cancer.

Conclusion

The high BMI-related burden of cancers is worsening, particularly in developing countries. Concerted action should be suggested to increase awareness of the harmful effects of high BMI and decrease the burden of disease attributable to high BMI, including cancer.
An LPAR5-antagonist that reduces nociception and increases pruriception
Authors: Langedijk Jacqueline , Araya Erika Ivanna , Barroso Amanda Ribeiro , Tolenaars Dagmar , Nazaré Marc , Belabed Hassane , Schoene Jens , Chichorro Juliana Geremias , Oude Elferink Ronald
Published: July 2022

Introduction

The G-protein coupled receptor LPAR5 plays a prominent role in LPA-mediated pain and itch signaling. In this study we focus on the LPAR5-antagonist compound 3 (cpd3) and its ability to affect pain and itch signaling, both in vitro and in vivo.

Methods

Nociceptive behavior in wild type mice was induced by formalin, carrageenan or prostaglandin E2 (PGE2) injection in the hind paw, and the effect of oral cpd3 administration was measured. Scratch activity was measured after oral administration of cpd3, in mice overexpressing phospholipase A2 (sPLA2tg), in wild type mice (WT) and in TRPA1-deficient mice (Trpa1 KO). In vitro effects of cpd3 were assessed by measuring intracellular calcium release in HMC-1 and HEK-TRPA1 cells.

Results

As expected, nociceptive behavior (induced by formalin, carrageenan or PGE2) was reduced after treatment with cpd3. Unexpectedly, cpd3 induced scratch activity in mice. In vitro addition of cpd3 to HEK-TRPA1 cells induced an intracellular calcium wave that could be inhibited by the TRPA1-antagonist A-967079. In Trpa1 KO mice, however, the increase in scratch activity after cpd3 administration was not reduced.

Conclusions

Cpd3 has in vivo antinociceptive effects but induces scratch activity in mice, probably by activation of multiple pruriceptors, including TRPA1. These results urge screening of antinociceptive candidate drugs for activity with pruriceptors.
Intraosseous access in neonates is feasible and safe – An analysis of a prospective nationwide surveillance study in Germany
Authors: Schwindt Eva , Pfeiffer Daniel , Gomes Delphina , Brenner Sebastian , Schwindt Jens-Christian , Hoffmann Florian , Olivieri Martin
Published: July 2022

Background

This was a prospective surveillance study to investigate reports on the safety and frequency of use of intraosseous (IO) access in neonates.

Methods

Over a two-year period, paediatric hospitals in Germany were asked to report all cases of IO access to the nationwide Surveillance Unit for Rare Paediatric Diseases (ESPED). Hospitals reporting a case submitted responses via an anonymised electronic questionnaire, providing details on indication, success rate, system used, location, duration to first successful IO access, complications, alternative access attempts and short-term outcome. We present a subset of data for IO use in infants of less than 28 days.

Results

A total of 161 neonates (145 term and 16 preterm born infants) with 206 IO access attempts were reported. In 146 neonates (91%), IO access was successfully established, and success was achieved with the first attempt in 109 neonates (75%). There was no significant impact of gestational age or provider’s educational level on success rates. In 71 infants with successful IO access (79%), the estimated duration of placement was less than 3 min. The proximal tibia was the predominant site used. A semiautomatic battery-driven device was used in 162 attempts (88%). The most often applied medications via IO access were crystalloid fluid and adrenaline. Potentially severe complications occurred in 9 patients (6%).

Conclusion

Within this surveillance study, IO access in neonates was feasible and safe. IO access is an important alternative for vascular access in neonates.
Population Pharmacokinetics of Cyclosporine in Chinese Pediatric Patients With Acquired Aplastic Anemia
Authors: Gao Xuan , Bian Zhu-Li , Qiao Xiao-Hong , Qian Xiao-Wen , Li Jun , Shen Guo-Mei , Miao Hui , Yu Yi , Meng Jian-Hua , Zhu Xiao-Hua , Jiang Jun-Ye , Le Jun , Yu Ling , Wang Hong-Sheng , Zhai Xiao-Wen
Published: July 2022

Cyclosporine (CsA) is a component of the first-line treatment for acquired aplastic anemia (acquired AA) in pediatric patients. This study aimed to develop a population pharmacokinetic (PK) model of CsA in Chinese pediatric patients with acquired AA to inform individual dosage regimens. A total of 681 CsA whole blood concentrations and laboratory data of 157 pediatric patients with acquired AA were retrospectively collected from two hospitals in Shanghai. A nonlinear mixed-effect model approach was used to build the population PK model. Potential covariate effects of age, body weight, and biochemical measurements (renal and liver functions) on CsA PK disposition were evaluated. Model fit was assessed using the basic goodness of fit and a visual predictive check. The CsA concentration data were accurately described using a two-compartment disposition model with first-order absorption and elimination. Body weight value was implemented as a fixed allometric function on all clearance and volume of distribution parameters. Total bilirubin level was identified as a significant covariate on apparent clearance (CL/F), with a 1.07% reduction per 1 nmol/L rise in total bilirubin level. The final estimates for CL/F and central volume (Vc/F) were 29.1 L/h and 325 L, respectively, for a typical 28 kg child. Other covariates (e.g., gender, age, albumin, hemoglobin, hematocrit, serum creatinine, and concomitant medication) did not significantly affect the PK properties of CsA. This population PK model, along with a maximum a posteriori Bayesian approach, could estimate individual PK parameters in pediatric patients with acquired AA to conduct individual CsA therapy.
Circadian Oscillations in the Murine Preoptic Area Are Reset by Temperature, but Not Light
Authors: Díaz Nicolás M. , Gordon Shannon A. , Lang Richard A. , Buhr Ethan D.
Published: July 2022

Mammals maintain their internal body temperature within a physiologically optimal range. This involves the regulation of core body temperature in response to changing environmental temperatures and a natural circadian oscillation of internal temperatures. The preoptic area (POA) of the hypothalamus coordinates body temperature by responding to both external temperature cues and internal brain temperature. Here we describe an autonomous circadian clock system in the murine ventromedial POA (VMPO) in close proximity to cells which express the atypical violet-light sensitive opsin, Opn5. We analyzed the light-sensitivity and thermal-sensitivity of the VMPO circadian clocks ex vivo. The phase of the VMPO circadian oscillations was not influenced by light. However, the VMPO clocks were reset by temperature changes within the physiological internal temperature range. This thermal-sensitivity of the VMPO circadian clock did not require functional Opn5 expression or a functional circadian clock within the Opn5-expressing cells. The presence of temperature-sensitive circadian clocks in the VMPO provides an advancement in the understanding of mechanisms involved in the dynamic regulation of core body temperature.
The Akebia Genus as a Novel Forest Crop: A Review of Its Genetic Resources, Nutritional Components, Biosynthesis, and Biological Studies
Authors: Huang Ping , Zang Fengqi , Li Changhong , Lin Furong , Zang Dekui , Li Bin , Zheng Yongqi
Published: July 2022

The genus Akebia belongs to the Lardizabalaceae family and comprises five species that are primarily distributed in East Asia. Plants of the Akebia genus comprise deciduous and semi-evergreen perennial twining vines that have been used in Chinese herbal medicine for at least 2000 years. The plants of this genus have the potential to form a novel forest crop with high nutritional and economic value because their fruit has a delicious sweet taste and rich nutrient components. In this study, we organized, analyzed, and evaluated the available published scientific literature on the botanical, ecological, and phytochemical characteristics of Akebia plants. Based on these studies, we briefly introduced botanical and ecological characteristics and focused on reviewing the development and utilization of wild genetic resources in the genus Akebia. We further explored the genus' rich nutritional components, such as triterpenes, flavonoids, polyphenols, polysaccharides, and fatty acids, and their potential use in food and health improvement applications. In addition, several papers describing advances in biotechnological research focusing on micropropagation, nutrient biosynthesis, and fruit ripeness were also included. This review provides comprehensive knowledge of the Akebia genus as a new forest crop for food and fruit utilization, and we also discuss future breeding and research prospects.
Impact of different levels of zinc and nitrogen on growth, productivity, and quality of aromatic rice cultivated under various irrigation regimes in two districts of Pakistan
Authors: Hasnain Zuhair , Khan Shahbaz , Nasrullah Fareeha , Mehmood Kashf , Ibrar Danish , Bashir Saqib , Bakhsh Ali , Aziz Irum , Rais Afroz , Farooq Naila , Irshad Sohail , Rashid Nabila , Alkahtani Jawaher , Elshikh Mohamed S.
Published: July 2022

Rice is a staple food for more than 50% of the global population and it is one of the most valuable cereal crops. To fulfill the dietary requirement of the ever-growing world population, an increase in per-unit production of rice is direly required. In Pakistan, it stands as the 2nd in consumption after wheat, which is a staple food. A huge gap is observed between yield potential and actual yield of the aromatic rice cultivars at a farmer-field level. The significant limitations responsible for this gap are shortage of irrigation water, inappropriate application of fertilizers, less plant population, deficiency of micronutrients, and improper and poor plant protection measures. A field study was planned to assess the yield response and quality attributes of aromatic rice to three levels of zinc (Zn) and nitrogen (N) under three irrigation regimes (8-, 12-, and 16-acre inches) in the Sheikhupura and Sargodha districts of Pakistan. Irrigation treatments significantly influenced the growth, yield, and quality attributes; however, maximum improvement was observed by the application of irrigation at 12-acre inches. Among the Zn treatments, application of Zn at 10 kg ha–1 was observed to be more responsive to improving the growth and quality parameters of aromatic rice crops. In the case of N treatments, application of N at 140 kg ha–1 produced the maximum total tillers, as well as productive tillers per hill, spikelets per panicle, leaf area index, leaf area duration, crop growth rate, total dry matter, harvest index, kernel length, kernel width, and 1,000-kernel weight. Application of N at 140 kg ha–1 not only improved the growth attributes but also increased the net assimilation rate, photosynthetically active radiation, and radiation use efficiency, with respect to total dry matter and kernel yield. The maximum percentage of normal kernels and minimum percentage of opaque, abortive, and chalky kernels were also recorded by application of N at 140 kg ha–1. The outcomes of current experiments depicted that application of irrigational water, zinc, and nitrogen at 12-acre inches, 10, and 140 kg ha–1, respectively, are responsible to achieve maximum resource utilization efficiency, along with increased yield and quality of rice.
A Survey on Depressive Symptoms and Its Correlates Amongst Physicians in Bangladesh During the COVID-19 Pandemic
Authors: Hasan M. Tasdik , Anjum Afifa , Biswas Md. Abdullah Al Jubayer , Hossain Sahadat , Alin Sayma Islam , Koly Kamrun Nahar , Safa Farhana , Alam Syeda Fatema , Rafi Md. Abdur , Podder Vivek , Hossain Md. Moynul , Trisa Tonima Islam , Azad Dewan Tasnia , Nodi Rhedeya Nury , Ashraf Fatema , Akther S. M. Quamrul , Ahmed Helal Uddin , McNaney Roisin
Published: July 2022

Aim

The aim of this study was to determine the presence of depressive symptoms and understand the potential factors associated with these symptoms among physicians in Bangladesh during the COVID-19 pandemic.

Methods

A cross-sectional study using an online survey was conducted in between April 21 and May 10, 2020, among physicians living in Bangladesh. Participants completed a series of demographic questions, COVID-19-related questions, and the Patient Health Questionnaire-9 (PHQ-9). Descriptive statistics (frequency, percentage, mean and standard deviation), test statistics (chi-squared test and logistic regression) were performed to explore the association between physicians’ experience of depression symptoms and other study variables. Stepwise binary logistic regression was followed while conducting the multivariable analysis.

Result

A total of 390 physicians completed the survey. Of them, 283 (72.6%) were found to be experiencing depressive symptoms. Predictors which were significantly associated with depressive symptoms were gender (with females more likely to experience depression than males), the presence of sleep disturbance, being highly exposed to media coverage about the pandemic, and fear around (a) COVID-19 infection, (b) being assaulted/humiliated by regulatory forces and (c) by the general public, while traveling to and from the hospital and treating patients during the countrywide lockdown.

Conclusion

The findings of this study demonstrate that there is a high prevalence of depressive symptom among physicians especially among female physicians in Bangladesh during the COVID-19 pandemic. Immediate, adequate and effective interventions addressing gender specific needs are required amid this ongoing crisis and beyond.
Kazakh “free women” grit—Chinese Kazakh women's clothing image in the context of multicultural integration of silk road
Authors: Xu Rui
Published: July 2022

In recent years, Chinese clothing cultural heritage and knowledge genealogy along the Silk Road have become the research headline attracting public attention. In particular, Kazakh clothing in Northwestern China has become the focus of today's traditional national culture. Kazakh, located at the intersection of the Silk Road, has an important position. The traditional clothing made by various social factors reflects the style and identity integration throughout history in cultures along the Silk Road, taking women's clothing as an example. Kazakh women are good at crafts, which is reflected in their strong grit. They gradually changed their role and traditional values, which has guided their social role. At the same time, it also reflects the awakening of Kazakh women's feminist consciousness to a large extent, and complies with Confucianism, along with the mainstream value of “advanced gender culture” of the world's outstanding civilization achievements. This paper summarizes the form, color, and patterns of Kazakh women's clothing by analyzing the garment, headdress, and footwear in Xinjiang Uygur Autonomous Region Museum, Aksai Kazakh National Museum, and Capital Museum of China. Through the analysis, this paper further probes into the multicultural integration between Kazakh and the regions along the Silk Road and interprets the idea represented by the forms, colors, and patterns in the clothing to promote the inheritance and development of traditional and regional cultures together with ethnic features along the Silk Road.
The effects of alphabetic literacy, linguistic-processing demand and tone type on the dichotic listening of lexical tones
Authors: Shao Jing , Zhang Caicai , Zhang Gaoyuan , Zhang Yubin , Pattamadilok Chotiga
Published: July 2022

Brain lateralization of lexical tone processing remains a matter of debate. In this study we used a dichotic listening paradigm to examine the influences of the knowledge of Jyutping (a romanization writing system which provides explicit Cantonese tone markers), linguistic-processing demand and tone type on the ear preference pattern of native tone processing in Hong Kong Cantonese speakers. While participants with little knowledge of Jyutping showed a previously reported left-ear advantage (LEA), those with a good level of Jyutping expertise exhibited either a right-ear advantage or bilateral processing during lexical tone identification and contour tone discrimination, respectively. As for the effect of linguistic-processing demand, while an LEA was found in acoustic/phonetic perception situations, this advantage disappeared and was replaced by a bilateral pattern in conditions that involved a greater extent of linguistic processing, suggesting an increased involvement of the left hemisphere. Regarding the effect of tone type, both groups showed an LEA in level tone discrimination, but only the Jyutping group demonstrated a bilateral pattern in contour tone discrimination. Overall, knowledge of written codes of tones, greater degree of linguistic processing and contour tone processing seem to influence the brain lateralization of lexical tone processing in native listeners of Cantonese by increasing the recruitment of the left-hemisphere language network.
Effects of the COVID-19 Pandemic on the Perception of Inclusion in School Education and Physical Activity Among Polish Students
Authors: Kostorz Karolina , Zwierzchowska Anna , Ziemba Mateusz
Published: July 2022

The coronavirus disease-2019 (COVID-19) pandemic impacted the lives of children and adolescents, leading to many changes in their routines, especially in education. Face-to-face physical education (PE) classes during COVID-19 were affected in organization, possibly conditioning students' participation, motivation, and learning. In the extreme conditions of the coronavirus, it may be assumed that daily physical activity became much less than before, partly because students are learning outside the school environment and PE lessons taught using remote forms do not fulfill their purpose. The aim of the study was to assess the pupil's perception of inclusion in school education during the remote learning caused by the COVID-19 pandemic and to compare the results with the control group. Moreover, the physical activity of respondents during social isolation due to the coronavirus was examined. The sample consisted of 111 pupils of both genders, aged 14–21 years. The Perceptions of Inclusion Questionnaire (PIQ) was used to measure the perception of inclusion in school education. The structure of the participants' physical activity was examined using the International Physical Activity Questionnaire-Long Form (IPAQ-LF) for adolescents. It was observed that the pupils' gender did not differentiate their perception of inclusion in school education. It was proved that respondents participating in research during the COVID-19 pandemic obtained statistically significantly lower results in the case of “emotional wellbeing in school” (p < 0.001; η2 = 0.07) but a higher mean was observed in relation to “social relationships with other pupils” (p = 0.04; η2 = 0.02) than the control group. Girls achieved a higher mean in the case of walk Metabolic Equivalent of Task (MET) (p = 0.02; η2 = 0.06) than boys. In addition, it was observed that the recommendation of vigorous physical activities was achieved by 37.78% of boys and 34.85% of girls. In turn, 69.70% of female pupils and 77.78% of male respondents met the recommendations for medium physical activities. It was also noted that 87.88% of girls and 86.67% of boys participating in the research achieved the recommendation for total physical activities. The analysis showed negligible and low positive correlations between examined variables.
The influence of modality on input, visuo-motor coordination, and execution in the advanced pianist's sight-reading processes
Authors: Qi Jing , Adachi Mayumi
Published: July 2022

In this study, we explored how the modality (major and minor) would affect the input (i. e., fixation), visuo-motor coordination (i.e., eye-hand span and time of performance), and execution (i.e., errors) in the advanced pianist's sight-reading processes, as well as relations among these three phases. Thirty-two advanced pianists with 5–54 years of piano training participated in the study. All participants sight-read three two-voice pieces in either major (n = 16) or minor (n = 16) mode while their eye movements were measured by an eye-tracking device (30 fps). All pieces were 20-measure long written in 4/4 m, adapted from unfamiliar Baroque pieces. Results showed that sight-readers fixated more frequently and tended to spend more time performing in a minor score than in a major score. This implies that modality of a score affects an efficiency of input and visuo-motor coordination in the advanced pianist's sight-reading. Spearman's correlation coefficients showed that errors were correlated positively with the number of fixations and the duration of performance. These results add more evidence to the notion that efficiencies in input and visuo-motor coordination are related to the accuracy in execution.
Teacher emotion and pedagogical decision-making in ESP teaching in a Chinese University
Authors: Zhao Hua , Li Danli , Zhong Yong
Published: July 2022

Teacher emotion has become an important issue in English language teaching as it is a crucial construct in understanding teachers' responses to institutional policies. The study explored teachers' emotion labor and its impact on teachers' pedagogical decision-making in English for Specific Purposes (ESP) teaching in a university of Traditional Chinese Medicine (TCM) in China. Drawing on a poststructural perspective, the study examined data from two rounds of semi-structured interviews, policy documents and teaching artifacts. The analysis of data revealed that the major emotion labor facing the participants revolved around students' disengagement in class. Teachers experienced mixed feelings of anticipation, disappointment, anger, and empathy toward students and distanced themselves from institutional feeling rules enforcing objective assessment of students' performance and punishing students for lack of engagement in class. The study found that teacher emotion labor served as the site for their pedagogical modifications. ESP teachers' beliefs in the importance of attending to students' needs become a powerful discourse in supporting teachers to strategically subvert institutional feeling rules and critically reflect on the dysfunctions of curriculum, orienting teachers' agentic actions in modifying pedagogical practices. We thus underscore this empowering discourse as the bridge to connect teachers' policy negotiation and their actual classroom practices. We also highlight teachers' pedagogical decision-making as a process of the interactions of teacher emotion, teachers' reflexive practices, and power relations. The study ended by suggesting more longitudinal research where teachers' beliefs as previously appropriated discourses could be examined comprehensively as they were both the construct of emotion labor and the potential subverting power in supporting teachers' pedagogical decision-making in policy negotiation.
An empirical investigation of firm performance through corporate governance and information technology investment with mediating role of corporate social responsibility: Evidence from Saudi Arabia telecommunication sector
Authors: Alfalah Adel Abdulmhsen , Muneer Saqib , Hussain Mazhar
Published: July 2022

This study intended to examine the effect of information technology (IT) investment and corporate governance mechanism on the performance of the Saudi telecommunication sector with mediating role of corporate social responsibility (CSR). A survey method was used to collect data from the targeted Saudi telecom firm. Results show that corporate governance practices, i.e., internal audit, internal audit committee, and internal board size, have a significant and positive relationship with firm performance. Furthermore, IT investment positively affects the performance of Saudi telecommunication firms. Moreover, CSR mediates the relationship among internal audit, internal audit committee, internal board size, IT investment, and firm performance. This study contributes to the body of knowledge regarding IT investment, corporate governance mechanism, corporate social responsibilities, and firm performance of telecommunication firms in emerging markets. Furthermore, this study will help the top management of the telecom firms to improve corporate governance and IT investment, which will be beneficial to enhance firm performance.
Remission of symptoms is not equal to functional recovery: Psychosocial functioning impairment in major depression
Authors: Yang Hao , Gao Shuzhan , Li Jiawei , Yu Haoran , Xu Jingren , Lin Chenchen , Yang Hua , Teng Changjun , Ma Hui , Zhang Ning
Published: July 2022

The ultimate goal of depression treatment is to achieve functional recovery. Psychosocial functioning is the main component of functional impairment in depressed patients. The concept of psychosocial functioning has an early origin; however, its concept and connotation are still ambiguous, which is the basic and key problem faced by the relevant research and clinical application. In this study, we start from the paradox of symptoms remission and functional recovery, describe the concept, connotation, and characteristics of psychosocial functioning impairment in depressed patients, and re-emphasize its importance in depression treatment to promote research and clinical applications related to psychosocial functioning impairment in depressed patients to achieve functional recovery.