bluuebunny/crossref_metadata_2025 huggingface dataset:
Seismic Vulnerability Assessment of a Medieval Urban Cluster Identified as a Complex Historical Palace: Palagio di Parte Guelfa in Florence
Authors: Caranti Anna , Cardinali Vieri , Ciuffreda Anna Livia , Coli Massimo , De Stefano Mario , Le Pera Elena , Tanganelli Marco
Published: December 2022

This paper presents the results of the application of a holistic procedure for a seismic vulnerability analysis of complex masonry aggregates which are ascribable as cultural heritage buildings. The methodological framework allowed us to properly identify the structural units comprising a historical palace through a hierarchical approach combining integrated geometrical and structural surveys with non-destructive (ND) and minor destructive (MD) techniques. The investigations were conducted on a significant palace located in the historical center of Florence, the Palagio di Parte Guelfa. The building covers an entire urban block, as it is constituted by many structural units developed over the centuries since the Roman period. The palace incorporates pre-existing structures, such as the Church of Santa Maria Sopra Porta and medieval terraced houses. Over the decades, different restorations and renovations have been carried out, including by Filippo Brunelleschi in the XV century and by Vasari in the XVI. Nowadays, the building constitutes an urban cluster. Our seismic vulnerability analysis took advantage of a specific knowledge path which was suitable for the identification of the different structural units of the palace. To this end, the historical evolution of the construction was traced through bibliographic research and ND surveys. We finally assessed the seismic performance of the different units according to different approaches, i.e., a rapid assessment based on simplified computations at the global scale, and a kinematic analysis for local phenomena.
Thermohygrometric Climate, Insects and Fungi in the Klosterneuburg Monastic Library
Authors: Brimblecombe Peter , Sterflinger Katja , Derksen Katharina , Haltrich Martin , Querner Pascal
Published: December 2022

The abundance of insect and fungal pests under a changing climate may threaten historic interiors, libraries and museums, with warmer, potentially more humid winters. This work examines local and indoor climate, insects and fungi in a historic library near Vienna. It reveals a mostly dry and cool environment for the storage of books, but few visitors to induce changes. Temperature and relative humidity have been monitored for 12 months (2021-07/2022-07), with 14 monitors positioned insect traps (blunder traps and some pheromone traps). Fungi in air, on surfaces and in settled dust were also sampled. Winter temperatures in library cupboards and behind shelves were slightly warmer (~1 °C) and more humid than in the library environment. Over the last decade there have been infestations of the biscuit beetles (Stegobium paniceum) but since treatment with sulfuryl difluoride, Anthrenus sp. have dominated. Silverfish are also present, but only in one corner. Fungal outbreaks have also been found, but over five years fungi in air samples have shifted from Penicillium commune and P. chrysogenum to Aspergillus sp. The stable environment at Klosterneuburg is suitable for books, yet insects and mould present suggests vigilance remains necessary, as some microenvironments (e.g., cupboards) can be at risk and there may be materials with high water content, hygroscopic or of nutritional value.
Acute Effects of the Interval and Duration of Intermittent Exercise on Arterial Stiffness in Young Men
Authors: Ren Jianchang , Xiao Haili , Wang Ping
Published: December 2022

We proved the hypothesis that intermittent exercise would have a better effect on arterial stiffness by shortening the duration of intervals and increasing the number of bouts. Twenty healthy male college students (20.4 ± 0.4 years) were randomly assigned to a quiet control (CON), 30 min continuous exercise (CE), long-interval intermittent exercise with long intervals (IELL), long-interval intermittent exercise with short intervals (IELS), and short-interval intermittent exercise with short intervals (IESS). The intensity was set to 45% of the heart rate reserve. The brachial-ankle pulse wave (baPWV) was measured at baseline (BL), 0 min post-exercise, 20 min post-exercise, 40 min post-exercise, and 60 min post-exercise. BaPWV changes (⊿baPWV) from the BL in the same tests were used for the analysis. ⊿baPWV did not change significantly in the CON. ⊿baPWV decreased significantly at 0, 20, and 40 min in all exercise tests. ⊿baPWV decreased significantly at 60 min in IELS and IESS. At 60 min, the ⊿baPWV of IELS and IESS was still significantly lower than that of CON and CE, and the ⊿baPWV of IESS was still significantly lower than that of IELS. Hence, shortening the intervals of intermittent exercise and increasing the number of repetitions may enhance the effect of improving arterial stiffness.
A Model of Panic Buying and Workforce under COVID-19
Authors: He Guohua , Hu Zirun
Published: December 2022

Allowing there to be an undersupply of medical resources and infection amid the social workforce, this paper proposes a theory to show how panic buying is induced and how bad the workforce status could be. By developing a novel general equilibrium model, we find that for any retail price that is higher than the buyer’s reserve value, the buying competition will be induced and the medical resources supply will further be tightened. Moreover, if the transmission rate of COVID-19 surpasses the theoretical threshold that is proposed by this paper, the whole workforce in our simulated economy will inevitably be infected.
Determinants of Physical Activity in Older Adults in South-Eastern Poland
Authors: Wiśniowska-Szurlej Agnieszka , Ćwirlej-Sozańska Agnieszka , Wilmowska-Pietruszyńska Anna , Sozański Bernard
Published: December 2022

The aim of our study is to assess factors determining the uptake of physical activity (PA) by older people living in south-eastern Poland. This is a cross-sectional study. The study included 858 older people aged 75 and over living in south-eastern Poland. PA was assessed by asking about the time spent on any at least moderate PA per week and about doing planned strengthening exercises to improve muscle strength and muscular endurance. Functional status, disability and quality of life in older people were also assessed. Logistic regression models were used to identify the factors related to PA. In the study group, only 25.64% performed a minimum of 150 min of moderate-intensity exercise, while strengthening exercises were performed by 22.49%. The most important factors influencing the uptake of PA were age, number of chronic diseases, place of residence, education, social activity, housing conditions, quality of life and health status. In summary, the study population represents a low level of PA uptake, with the majority not meeting the World Health Organization recommendations for PA uptake by older people. Our findings suggest individualized efforts to promote public health and increase PA among older people over 75 years of age.
Integrated Analysis Revealed an Inflammatory Cancer-Associated Fibroblast-Based Subtypes with Promising Implications in Predicting the Prognosis and Immunotherapeutic Response of Bladder Cancer Patients
Authors: Chen Hualin , Yang Wenjie , Xue Xiaoqiang , Li Yingjie , Jin Zhaoheng , Ji Zhigang
Published: December 2022

Inflammatory cancer-associated fibroblasts (iCAFs) are closely related to progression, anticancer therapeutic resistance, and poor prognosis of bladder cancer (BCa). However, the functional role of iCAFs in BCa has been poorly studied. In our study, two BCa scRNA-seq datasets (GSE130001 and GSE146137) were obtained and integrated by the Seurat pipeline. Based on reported markers (COL1A1 and PDGFRA), iCAFs were identified and the related signature of 278 markers was developed. Following unsupervised consensus clustering, two molecular subtypes of TCGA-BLCA were identified and characterized by distinct dysregulated cancer hallmarks, immunological tumor microenvironments, prognoses, responses to chemotherapy/immunotherapy, and stemness. Subsequently, the robustness of the signature-based clustering, in terms of prognosis and therapeutic response prediction, was validated in a GEO-meta cohort with seven independent GEO datasets of 519 BCa patients, and three immune checkpoint inhibitor (ICI)-treated cohorts. Considering the heterogeneity, re-clustering of iCAFs was performed and a subpopulation, named “LOXL2+ iCAFs”, was identified. Co-culture CM derived from LOXL2 overexpression/silencing CAFs with T24 cells revealed that overexpression of LOXL2 in CAFs promoted while silencing LOXL2 inhibited the proliferation, migration, and invasion of T24 cells through IL32. Moreover, the positive correlation between LOXL2 and CD206, an M2 macrophage polarization marker, has been observed and validated. Collectively, integrated single-cell and bulk RNA sequencing analyses revealed an iCAF-related signature that can predict prognosis and response to immunotherapy for BCa. Additionally, the hub gene LOXL2 may serve as a promising target for BCa treatment.
The Challenges of O2 Detection in Biological Fluids: Classical Methods and Translation to Clinical Applications
Authors: Marassi Valentina , Giordani Stefano , Kurevija Andjela , Panetta Emilio , Roda Barbara , Zhang Nan , Azzolini Andrea , Dolzani Sara , Manko Dmytro , Reschiglian Pierluigi , Atti Mauro , Zattoni Andrea
Published: December 2022

Dissolved oxygen (DO) is deeply involved in preserving the life of cellular tissues and human beings due to its key role in cellular metabolism: its alterations may reflect important pathophysiological conditions. DO levels are measured to identify pathological conditions, explain pathophysiological mechanisms, and monitor the efficacy of therapeutic approaches. This is particularly relevant when the measurements are performed in vivo but also in contexts where a variety of biological and synthetic media are used, such as ex vivo organ perfusion. A reliable measurement of medium oxygenation ensures a high-quality process. It is crucial to provide a high-accuracy, real-time method for DO quantification, which could be robust towards different medium compositions and temperatures. In fact, biological fluids and synthetic clinical fluids represent a challenging environment where DO interacts with various compounds and can change continuously and dynamically, and further precaution is needed to obtain reliable results. This study aims to present and discuss the main oxygen detection and quantification methods, focusing on the technical needs for their translation to clinical practice. Firstly, we resumed all the main methodologies and advancements concerning dissolved oxygen determination. After identifying the main groups of all the available techniques for DO sensing based on their mechanisms and applicability, we focused on transferring the most promising approaches to a clinical in vivo/ex vivo setting.
The Immunomodulator Dimethyl Itaconate Inhibits Several Key Steps of Angiogenesis in Cultured Endothelial Cells
Authors: Vidal Isabel , Fernández-Florido Elena , Marrero Ana Dácil , Castilla Laura , R. Quesada Ana , Martínez-Poveda Beatriz , Medina Miguel Ángel
Published: December 2022

The dimethyl derivative of the immunomodulator itaconate has been previously shown to have anti-inflammatory, anti-oxidative, and immunomodulatory effects. In the present work, we evaluate the potential of dimethyl itaconate as an anti-angiogenic compound by using cultured endothelial cells and several in vitro assays that simulate key steps of the angiogenic process, including endothelial cell proliferation, migration, invasion, and tube formation. Our results show that dimethyl itaconate interferes with all the previously mentioned steps of the angiogenic process, suggesting that dimethyl itaconate behaves as an anti-angiogenic compound.
Hepatitis B and Hepatitis D Viruses: A Comprehensive Update with an Immunological Focus
Authors: Sausen Daniel G. , Shechter Oren , Bietsch William , Shi Zhenzhen , Miller Samantha M. , Gallo Elisa S. , Dahari Harel , Borenstein Ronen
Published: December 2022

Hepatitis B virus (HBV) and hepatitis delta virus (HDV) are highly prevalent viruses estimated to infect approximately 300 million people and 12–72 million people worldwide, respectively. HDV requires the HBV envelope to establish a successful infection. Concurrent infection with HBV and HDV can result in more severe disease outcomes than infection with HBV alone. These viruses can cause significant hepatic disease, including cirrhosis, fulminant hepatitis, and hepatocellular carcinoma, and represent a significant cause of global mortality. Therefore, a thorough understanding of these viruses and the immune response they generate is essential to enhance disease management. This review includes an overview of the HBV and HDV viruses, including life cycle, structure, natural course of infection, and histopathology. A discussion of the interplay between HDV RNA and HBV DNA during chronic infection is also included. It then discusses characteristics of the immune response with a focus on reactions to the antigenic hepatitis B surface antigen, including small, middle, and large surface antigens. This paper also reviews characteristics of the immune response to the hepatitis D antigen (including small and large antigens), the only protein expressed by hepatitis D. Lastly, we conclude with a discussion of recent therapeutic advances pertaining to these viruses.
Candida Administration in 5/6 Nephrectomized Mice Enhanced Fibrosis in Internal Organs: An Impact of Lipopolysaccharide and (1→3)-β-D-Glucan from Leaky Gut
Authors: Tungsanga Somkanya , Udompornpitak Kanyarat , Worasilchai Jesadakorn , Ratana-aneckchai Tharit , Wannigama Dhammika Leshan , Katavetin Pisut , Leelahavanichkul Asada
Published: December 2022

Uremic toxins and gut dysbiosis in advanced chronic kidney disease (CKD) can induce gut leakage, causing the translocation of gut microbial molecules into the systemic circulation. Lipopolysaccharide (LPS) and (1→3)-β-D-glucan (BG) are the major gut microbial molecules of Gram-negative bacteria and fungi, respectively, and can induce inflammation in several organs. Here, the fibrosis in the kidney, liver, and heart was investigated in oral C. albicans-administered 5/6 nephrectomized (Candida-5/6 Nx) mice. At 20 weeks post 5/6 Nx, Candida-5/6 Nx mice demonstrated increased 24 h proteinuria, liver enzymes, and serum cytokines (TNF-α, IL-6, and IL-10), but not weight loss, systolic blood pressure, hematocrit, serum creatinine, or gut-derived uremic toxins (TMAO and indoxyl sulfate), compared to in 5/6 Nx alone. The gut leakage in Candida-5/6 Nx was more severe, as indicated by FITC-dextran assay, endotoxemia, and serum BG. The areas of fibrosis from histopathology, along with the upregulated gene expression of Toll-like receptor 4 (TLR-4) and Dectin-1, the receptors for LPS and BG, respectively, were higher in the kidney, liver, and heart. In vitro, LPS combined with BG increased the supernatant IL-6 and TNF-α, upregulated the genes of pro-inflammation and pro-fibrotic processes, Dectin-1, and TLR-4 in renal tubular (HK-2) cells and hepatocytes (HepG2), when compared with LPS or BG alone. This supported the pro-inflammation-induced fibrosis and the possible LPS–BG additive effects on kidney and liver fibrosis. In conclusion, uremia-induced leaky gut causes the translocation of gut LPS and BG into circulation, which activates the pro-inflammatory and pro-fibrotic pathways, causing internal organ fibrosis. Our results support the crosstalk among several organs in CKD through a leaky gut.
Molecular Regulation of Concomitant Lower Urinary Tract Symptoms and Erectile Dysfunction in Pelvic Ischemia
Authors: Tarcan Tufan , Choi Han-Pil , Azadzoi Kazem M.
Published: December 2022

Aging correlates with greater incidence of lower urinary tract symptoms (LUTS) and erectile dysfunction (ED) in the male population where the pathophysiological link remains elusive. The incidence of LUTS and ED correlates with the prevalence of vascular risk factors, implying potential role of arterial disorders in concomitant development of the two conditions. Human studies have revealed lower bladder and prostate blood flow in patients with LUTS suggesting that the severity of LUTS and ED correlates with the severity of vascular disorders. A close link between increased prostatic vascular resistance and greater incidence of LUTS and ED has been documented. Experimental models of atherosclerosis-induced chronic pelvic ischemia (CPI) showed increased contractile reactivity of prostatic and bladder tissues, impairment of penile erectile tissue relaxation, and simultaneous development of detrusor overactivity and ED. In the bladder, short-term ischemia caused overactive contractions while prolonged ischemia provoked degenerative responses and led to underactivity. CPI compromised structural integrity of the bladder, prostatic, and penile erectile tissues. Downstream molecular mechanisms appear to involve cellular stress and survival signaling, receptor modifications, upregulation of cytokines, and impairment of the nitric oxide pathway in cavernosal tissue. These observations may suggest pelvic ischemia as an important contributing factor in LUTS-associated ED. The aim of this narrative review is to discuss the current evidence on CPI as a possible etiologic mechanism underlying LUTS-associated ED.
Piezoelectric and Magnetoelectric Effects of Flexible Magnetoelectric Heterostructure PVDF-TrFE/FeCoSiB
Authors: Wen Dandan , Chen Xia , Huang Fuchao , Zhang Jingbo , Yang Pingan , Li Renpu , Lu Yi , Liu Yu
Published: December 2022

Flexible polymer-based magnetoelectric (ME) materials have broad application prospects and are considered as a new research field. In this article, FeCoSiB thin films were deposited on poly(vinylidene fluoride-co-trifluoroethylene) (PVDF-TrFE) substrate by DC magnetron sputtering. The structure of PVDF-TrFE/FeCoSiB heterostructure thin films was similar to 2-2. Under a bias magnetic field of 70 Oe, the composites have a dramatically increased ME voltage coefficient as high as 111 V/cm⋅Oe at a frequency of about 85 kHz. The piezoelectric coefficient of PVDF-TrFE thin films is 34.87 pC/N. The surface morphology of PVDF-TrFE thin films were studied by FESEM, and the results of XRD and FTIR showed that the β-phase of PVDF-TrFE thin films was dominant. Meanwhile, the effects of different heating conditions on the crystallization and piezoelectric properties of PVDF-TrFE films were also studied. The flexible ME heterojunction composite has a significant ME voltage coefficient and excellent piezoelectric properties at room temperature, which allows it to be a candidate material for developing flexible magnetoelectric devices.
Polyphosphazene-Based Biomaterials for Biomedical Applications
Authors: Jin Geun-Woo , Rejinold N. Sanoj , Choy Jin-Ho
Published: December 2022

Recently, synthetic polymers have attracted great interest in the field of biomedical science. Among these, polyphosphazenes (PPZs) are regarded as one of the most promising materials, due to their structural flexibility and biodegradability compared to other materials. PPZs have been developed through numerous studies. In particular, multi-functionalized PPZs have been proven to be potential biomaterials in various forms, such as nanoparticles (NPs) and hydrogels, through the introduction of various functional groups. Thus, PPZs have been applied for the delivery of therapeutic molecules (low molecular weight drugs, genes and proteins), bioimaging, phototherapy, bone regeneration, dental liners, modifiers and medical devices. The main goal of the present review is to highlight the recent and the most notable existing PPZ-based biomaterials for aforementioned applications, with future perspectives in mind.
Investigation of Structural Alterations in Inherited Retinal Diseases: A Quantitative SD-OCT-Analysis of Retinal Layer Thicknesses in Light of Underlying Genetic Mutations
Authors: Gersch Julia , Hufendiek Katerina , Delarocque Julien , Framme Carsten , Jacobsen Christina , Stöhr Heidi , Kellner Ulrich , Hufendiek Karsten
Published: December 2022

Inherited retinal diseases can result from various genetic defects and are one of the leading causes for blindness in the working-age population. The present study aims to provide a comprehensive description of changes in retinal structure associated with phenotypic disease entities and underlying genetic mutations. Full macular spectral domain optical coherence tomography scans were obtained and manually segmented in 16 patients with retinitis pigmentosa, 7 patients with cone–rod dystrophy, and 7 patients with Stargardt disease, as well as 23 age- and sex-matched controls without retinal disease, to assess retinal layer thicknesses. As indicated by generalized least squares models, all IRDs were associated with retinal thinning (p < 0.001), especially of the outer nuclear layer (ONL, p < 0.001). Except for the retinal nerve fiber layer, such thinning was associated with a reduced visual acuity (p < 0.001). These advances in our understanding of ultrastructural retinal changes are important for the development of gene-, cell-, and optogenetic therapy. Longitudinal studies are warranted to describe the temporal component of those changes.
Telomere Length: A Cardiovascular Biomarker and a Novel Therapeutic Target
Authors: Sagris Marios , Theofilis Panagiotis , Antonopoulos Alexios S. , Tsioufis Konstantinos , Tousoulis Dimitris
Published: December 2022

Coronary artery disease (CAD) is a multifactorial disease with a high prevalence, particularly in developing countries. Currently, the investigation of telomeres as a potential tool for the early detection of the atherosclerotic disease seems to be a promising method. Telomeres are repetitive DNA sequences located at the extremities of chromosomes that maintain genetic stability. Telomere length (TL) has been associated with several human disorders and diseases while its attrition rate varies significantly in the population. The rate of TL shortening ranges between 20 and 50 bp and is affected by factors such as the end-replication phenomenon, oxidative stress, and other DNA-damaging agents. In this review, we delve not only into the pathophysiology of TL shortening but also into its association with cardiovascular disease and the progression of atherosclerosis. We also provide current and future treatment options based on TL and telomerase function, trying to highlight the importance of these cutting-edge developments and their clinical relevance.
Investigation of the Interaction between Aloe vera Anthraquinone Metabolites and c-Myc and C-Kit G-Quadruplex DNA Structures
Authors: Dallavalle Sabrina , Artali Roberto , Princiotto Salvatore , Musso Loana , Borgonovo Gigliola , Mazzini Stefania
Published: December 2022

G-quadruplexes are nucleotide sequences present in the promoter region of numerous oncogenes, having a key role in the suppression of gene transcription. Recently, the binding of anthraquinones from Aloe vera to G-quadruplex structures has been studied through various physico-chemical techniques. Intrigued by the reported results, we investigated the affinity of aloe emodin, aloe emodin-8-glucoside, and aloin to selected G-quadruplex nucleotide sequences by NMR spectroscopy. The structural determinants for the formation of the ligand/nucleotide complexes were elucidated and a model of the interactions between the tested compounds and C-Kit and c-Myc G-quadruplex DNA structures was built by integrated NMR and molecular modeling studies. Overall, the obtained results confirmed and implemented the previously reported findings, pointing out the complementarity of the different approaches and their contribution to a more detailed overview of the ligand/nucleotide complex formation. Furthermore, the proposed models of interaction could pave the way to the design of new nature-derived compounds endowed with increased G-quadruplex stabilizing activity.
Ovarian Microbiota, Ovarian Cancer and the Underestimated Role of HPV
Authors: Cazzaniga Massimiliano , Cardinali Marco , Di Pierro Francesco , Bertuccioli Alexander
Published: December 2022

In recent years, many studies have highlighted the possible close correlation between human diseases and definite patterns of microbial organisms colonizing various organs. Even at sites traditionally considered sterile, such as the upper female reproductive tract (FRT), it is now well-recognized as hosting a low biomass of different bacterial phyla. Additionally, the data from recent studies highlight a possible link between lower and upper FRT dysbiosis with a potential predisposition to cervical and ovarian cancer. Acinetobacter, chlamydia, increased mycoplasma, and lactobacillary scarcity in the upper FRT have all been linked to a predisposition to ovarian cancer. Additionally, a high-diversity vaginal community state type (CST) is linked to the presence and persistence of high-risk human papillomavirus (HPV), resulting in decreased cellular p53 activity and a reduction in the immune activity of T lymphocytes, resulting in cervical and ovarian cancer predisposition. While these findings are still far from being clarified in all aspects, in patients with multiple risk factors for ovarian cancer, a Lactobacillus crispatus treatment with a product with a proven ability to restore a favorable CST should be considered as an add-on therapy.
Ataxia-Telangiectasia Mutated Loss of Heterozygosity in Melanoma
Authors: Pastorino Lorenza , Dalmasso Bruna , Allavena Eleonora , Vanni Irene , Ugolini Filippo , Baroni Gianna , Croce Michela , Guadagno Antonio , Cabiddu Francesco , Andreotti Virginia , Bruno William , Zoppoli Gabriele , Ferrando Lorenzo , Tanda Enrica Teresa , Spagnolo Francesco , Menin Chiara , Gangemi Rosaria , Massi Daniela , Ghiorzo Paola
Published: December 2022

ATM germline pathogenic variants were recently found enriched in high-risk melanoma patients. However, ATM loss of heterozygosity (LOH) has never been investigated in melanoma and, therefore, a causal association with melanoma development has not been established yet. The purpose of this study was to functionally characterize 13 germline ATM variants found in high-risk melanoma patients—and classified by in silico tools as pathogenic, uncertain significance, or benign—using multiple assays evaluating ATM/pATM expression and/or LOH in melanoma tissues and cell lines. We assessed ATM status by Immunohistochemistry (IHC), Western Blot, Whole-Exome Sequencing/Copy Number Variation analysis, and RNA sequencing, supported by Sanger sequencing and microsatellite analyses. For most variants, IHC results matched those obtained with in silico classification and LOH analysis. Two pathogenic variants (p.Ser1135_Lys1192del and p.Ser1993ArgfsTer23) showed LOH and complete loss of ATM activation in melanoma. Two variants of unknown significance (p.Asn358Ile and p.Asn796His) showed reduced expression and LOH, suggestive of a deleterious effect. This study, showing a classic two-hit scenario in a well-known tumor suppressor gene, supports the inclusion of melanoma in the ATM-related cancer spectrum.
Effects of Cardiac Contractility Modulation Electrodes on Tricuspid Regurgitation in Patients with Heart Failure with Reduced Ejection Fraction: A Pilot Study
Authors: Masarone Daniele , Kittleson Michelle M. , De Vivo Stefano , D’Onofrio Antonio , Rao Ishu , Ammendola Ernesto , Errigo Vittoria , Martucci Maria L. , Nigro Gerardo , Pacileo Giuseppe
Published: December 2022

Background: Cardiac contractility modulation (CCM) is an innovative therapy for heart failure with reduced ejection fraction delivered by a cardiac implantable device (Optimizer Smart®). One of the most prominent periprocedural complications common to all cardiac implantable devices (CIDs) is tricuspid regurgitation (TR) due to the placement of the right ventricular endocardial leads. To date, no published studies have assessed the changes in the TR degree in patients with heart failure with reduced ejection fraction (HFrEF) who received an implantable cardioverter-defibrillator (ICD) after the implantation of cardiac contractility modulation therapy devices. Objective: This study aimed to evaluate the effect of the implantation of the trans-tricuspid leads required to deliver CCM therapy on the severity of TR in patients with HFrEF who previously underwent ICD implantation. Methods: We enrolled 30 HFrEF patients who underwent CCM therapy between November 2020 and October 2021. For all the patients, echocardiographic evaluations of TR were performed according to current guidelines 24 h before and six months after the Optimizer Smart® implant was applied. Results: At the 6-month follow-up, the grade of TR remained unchanged compared to the preimplant grade. The value of the vena contracta (VC) of TR was 0.40 ± 0.19 cm in the preimplant period and 0.45 ± 0.21 cm at the 6-month follow-up (p = 0.33). Similarly, the TR proximal isovelocity surface area (PISA) radius value was unchanged at follow-up (0.54 ± 0.22 cm vs. 0.62 ± 0.20 cm; p = 0.18). No statistically significant difference existed between the preimplant VC and PISA radius values, irrespective of the device type. Conclusions: The implantation of right ventricular electrodes for the delivery of CCM therapy did not worsen tricuspid regurgitation in patients with HFrEF and ICD.
Assessment of Quality of Life in Head-and-Neck Oncologic Patients with Intraoral Soft-Tissue Defects Reconstructed with Buccinator Myomucosal Flap
Authors: Agea Martínez Marc , Antúnez-Conde Raúl , Navarro Cuéllar Carlos , Tousidonis Rial Manuel , Navarro Cuéllar Ignacio , López López Ana María , Gascón Alonso Dafne , Sada Urmeneta Ángela , Zamorano-León José J.
Published: December 2022

The aim of this study is to evaluate the functional outcomes and quality of life (QoL) in oncologic patients with intraoral defects reconstructed with the buccinator myomucosal flap. A retrospective study was performed involving 39 patients with intraoral soft-tissue defects, reconstructed with a buccinator myomucosal flap during a six-year period. Patients completed the European Organization for Research and Treatment of Cancer questionnaires, the standard questionnaire (QLQ-C30) and the head-and-neck specific module (QLQ-H&N35). Thirty-nine patients with a mean age of 61.23 ± 15.80 years were included in the study. Thirty-three patients were diagnosed with an oncological condition (84.61%). Six patients (15.38%) developed orosinusal communication and underwent extensive debridement. The median global-health-status score was 79.27 and emotional performance was the lowest scoring, with a mean score of 76.93. As for the symptom items, the most outstanding were dental problems (33.33), oral opening (31.62) and dry mouth (37.61), followed by sticky saliva (24.79), problems with social eating (21.15) and pain (19.87). The most significant symptoms were radiotherapy-related adverse effects such as pain, fatigue, dental problems and dry mouth. Patients reconstructed with the buccinator myomucosal flap develop a good quality of life for all types of activities, and a correct function and aesthetics. Postoperative radiotherapy is associated with a poorer quality of life, and can lead to impairment of several symptoms such as swallowing, oral opening and dry mouth.
Systemic Oxidative Stress in Women with Ovarian and Pelvic Endometriosis: Role of Hormonal Therapy
Authors: Biasioli Anna , Xholli Anjeza , Previtera Francesca , Balzano Alessandro , Capodicasa Valentina , Tassi Alice , Londero Ambrogio P. , Cagnacci Angelo
Published: December 2022

This study was performed to evaluate the systemic oxidative stress balance in women with either ovarian or deep infiltrating endometriosis (DIE) and any alterations of the same during hormone therapy. Free oxygen radicals (FORT) and free oxidant radical defense (FORD) were measured in the capillary blood of 24 women without endometriosis, 26 women with endometrioma, and 26 women with DIE with or without endometrioma. Endometriosis was diagnosed by clinical and ultrasound assessment. Dietary factors, lifestyle habits, and intake of any substances interfering with the oxidative status were recorded. Women were prescribed contraceptive hormones, and the baseline assessments were repeated at the 3rd month of use, revealing a higher oxidative stress balance (FORT/FORD) in women with endometriosis than in controls (4.75 ± 4.4 vs. 2.79 ± 2.2; p = 0.05). The highest values were found in women with DIE (5.34 ± 4.6; p = 0.028 vs. controls). Regression analysis revealed an independent link between FORT/FORD and endometrioma (b 2.874, 95% CI 0.345, 5.403; p = 0.027) and DIE (b 4.419, 95% CI 1.775, 7.064; p = 0.001) but a negative correlation with HDL-cholesterol (b −0.063, 95% CI −0.125, −0.002; p = 0.043). In controls, the hormone therapy increased FORT (p = 0.003), but also FORD (p = 0.012), with the FORT/FORD balance remaining stable (2.72 ± 2.2 vs. 2.73 ± 1.8; p = 0.810). In women with endometriosis, FORT remained unchanged, but FORD increased (p = 0.004), and the FORT/FORD ratio significantly decreased (4.75 ± 4.4 vs. 2.57 ± 1.76; p = 0.002) to values similar to the control levels. These data indicate that systemic oxidative stress balance increased in women with endometriosis, particularly in those with DIE. The hormonal therapy did not change the oxidative stress balance in control women but significantly improved it in women with endometriosis, particularly those suffering from DIE.
Trends in Hospitalization and Economic Impact of Percutaneous Kyphoplasty in Italy
Authors: Longo Umile Giuseppe , Papalia Rocco , Denaro Luca , De Salvatore Sergio , Ruzzini Laura , Piergentili Ilaria , Denaro Vincenzo
Published: December 2022

Vertebral Fractures (VFs) caused by osteoporosis are the most typical reason for performing Percutaneous Kyphoplasty (PK). Globally, VF prevalence is not well described in the literature. In Europe, only Sweden has an accurate record of the incidence of this type of fracture. Moreover, the exact incidence of the PK procedure is not reported. Therefore, the annual patterns and financial burden of PK in Europe is not well known, and it could be may better understood by examining national registers. For surgeons, lawmakers, hospital administrators, and the healthcare system, determining the annual national costs of this treatment is helpful. The National Hospital Discharge Reports (SDOs) submitted to the Italian Ministry of Health during the years covered by this study (2009–2015) were used. A total of 13,113 kyphoplasties were performed in Italy, with a prevalence of 3.6 procedures for every 100,000 Italian inhabitants over 15 years. The average age of patients was 68.28 (±12.9). Females represented the majority of patients undergoing PK procedures (68.6%). The median length of hospital stay was 5.33 days. The prevalence of PK procedures increased from 2009 to 2015, while the average days of hospitalization decreased. Older patients were most at risk in undergoing PK procedures. Reporting the national data on PK in Italy could also help compare the findings across nations. The current study aims to determine the trends of PK hospitalisation and patient features in Italy.
Fatigue, Depression, and Anxiety in Patients with COPD, Asthma and Asthma-COPD Overlap
Authors: Homętowska Henryka , Klekowski Jakub , Świątoniowska-Lonc Natalia , Jankowska-Polańska Beata , Chabowski Mariusz
Published: December 2022

Introduction. Asthma and COPD are extremely common respiratory diseases that have a serious impact on people’s lives around the world. A disease characterized by symptoms characteristic for asthma and COPD is called asthma-COPD overlap (ACO). Fatigue and certain psychological disorders such as anxiety and depression are important comorbidities in these diseases. The purpose of this study was to assess the prevalence of fatigue, anxiety, and depression in patients with asthma, COPD, and ACO and to also consider their mutual correlations. Material and Methods. A total of 325 patients were enrolled in the study. There were 159 women and 166 men and their mean age was 63. Two standardized questionnaires were used: the Modified Fatigue Impact Scale (MFIS) and the Hospital Anxiety and Depression Scale (HADS). Results. The mean total MFIS score for all patients was 33.03. Patients with asthma generally scored lower than patients with COPD and ACO. There were no statistical differences in the HADS for anxiety between the groups, although around half of the patients registered a score indicating some level of disorder. Patients with COPD and ACO were proven to suffer more from depression than patients with asthma. The HADS and MFIS scores were found to correlate significantly and positively. Conclusions. Our study showed that patients with COPD, asthma, and ACO generally suffered from an increased level of fatigue and depression. Anxiety was high in all groups, but it was at a similar level for patients suffering from each of the three diseases under consideration. It is important to treat the physical symptoms as well as the psychological disorders since they greatly impact on the patient outcomes.
Relationship of the Quanta-to-Energy Ratio of Photosynthetically Active Radiation with Chlorophyll-a in Case I Seawater
Authors: Wang Weibo , Zheng Jianhua , Jing Chunsheng , Zhao Jinping
Published: December 2022

A predetermined quanta-to-energy ratio is often used in many ecosystem models to transform photosynthetically radiant flux density into photosynthetic photon flux density when light penetrates seawater. The calculation formula of the ratio is reduced in this study as the product of a constant and the defined principal wavelength. The principal wavelength is discussed in this paper and may be expressed as an exponential function using theoretical reasoning. The deviation of the principal wavelength is defined as the difference between two natural logarithms of the observed principal wavelength in real seawater and the simulated principal wavelengths of pure seawater under the same surface solar radiation. The deviation has a quasi-linear relationship with the non-water diffuse attenuation coefficient at 490 nm, which is related to the chlorophyll-a concentration. A semi-empirical formula between the deviation and chlorophyll-a concentration is established with a determination coefficient of more than 84%. In pure seawater, an empirical formula for the principal wavelength profile is also constructed. The parameterization formula for the quanta-to-energy ratio is provided as a function of the chlorophyll-a concentration and the surface principal wavelength. When applied to in situ observations, the statistical relative inaccuracy is found to be less than 10%. The parameterization formula can be applied to the ecosystem models to realize the transformation between photos and energy in the PAR waveband.
Fusarium oxysporum Casein Kinase 1, a Negative Regulator of the Plasma Membrane H+-ATPase Pma1, Is Required for Development and Pathogenicity
Authors: Mariscal Melani , Miguel-Rojas Cristina , Hera Concepción , Fernandes Tânia R. , Di Pietro Antonio
Published: December 2022

Like many hemibiotrophic plant pathogens, the root-infecting vascular wilt fungus Fusarium oxysporum induces an increase in the pH of the surrounding host tissue. How alkalinization promotes fungal infection is not fully understood, but recent studies point towards the role of cytosolic pH (pHc) and mitogen-activated protein kinase (MAPK) signaling. In fungi, pHc is mainly controlled by the essential plasma membrane H+-ATPase Pma1. Here we created mutants of F. oxysporum lacking casein kinase 1 (Ck1), a known negative regulator of Pma1. We found that the ck1Δ mutants have constitutively high Pma1 activity and exhibit reduced alkalinization of the surrounding medium as well as decreased hyphal growth and conidiation. Importantly, the ck1Δ mutants exhibit defects in hyphal chemotropism towards plant roots and in pathogenicity on tomato plants. Thus, Ck1 is a key regulator of the development and virulence of F. oxysporum.
Phylogenetic Analyses of Hydnobolites and New Species from China
Authors: Wan Shan-Ping , Huang Lan-Lan , Cui Meng-Jin , Yu Cheng-Jin , Liu Wei , Wang Rui , Shi Xiao-Fei , Yu Fu-Qiang
Published: December 2022

Hydnobolites is an ectomycorrhizal fungal genus with hypogeous ascomata in the family Pezizaceae (Pezizales). Molecular analyses of Hydnobolites using both single (ITS) and concatenated gene datasets (ITS-nLSU) showed a total of 223 sequences, including 92 newly gained sequences from Chinese specimens. Phylogenetic results based on these two datasets revealed seven distinct phylogenetic clades. Among them, the ITS phylogenetic tree confirmed the presence of at least 42 phylogenetic species in Hydnobolites. Combined the morphological observations with molecular analyses, five new species of Hydnobolites translucidus sp. nov., H. subrufus sp. nov., H. lini sp. nov., H. sichuanensis sp. nov. and H. tenuiperidius sp. nov., and one new record species of H. cerebriformis Tul., were illustrated from Southwest China. Macro- and micro-morphological analyses of ascomata revealed a few, but diagnostic differences between the H. cerebriformis complex, while the similarities of the ITS sequences ranged from 94.4 to 97.2% resulting in well-supported clades.
Machine Learning Based Prediction of Gamma Passing Rate for VMAT Radiotherapy Plans
Authors: Sadowski Bartłomiej , Milewska Karolina , Ginter Józef
Published: December 2022

The use of machine learning algorithms (ML) in radiotherapy is becoming increasingly popular. More and more groups are trying to apply ML in predicting the so-called gamma passing rate (GPR). Our team has developed a customized approach of using ML algorithms to predict global GPR for electronic portal imaging device (EPID) verification for dose different 2% and distance to agreement 2 mm criteria for VMAT dynamic plans. Plans will pass if the GPR is greater than 98%. The algorithm was learned and tested on anonymized clinical data from 13 months which resulted in more than 3000 treatment plans. The obtained results of GPR prediction are very interesting. Average specificity of the algorithm based on an ensemble of 50 decision tree regressors is 91.6% for our criteria. As a result, we can reduce the verification process by 50%. The novel approach described by our team can offer a new insight into the application of ML and neural networks in GPR prediction and dosimetry.
Langerhans Cells—Revising Their Role in Skin Pathologies
Authors: Neagu Monica , Constantin Carolina , Jugulete Gheorghita , Cauni Victor , Dubrac Sandrine , Szöllősi Attila Gábor , Zurac Sabina
Published: December 2022

Langerhans cells (LCs) constitute a cellular immune network across the epidermis. Because they are located at the skin barrier, they are considered immune sentinels of the skin. These antigen-presenting cells are capable of migrating to skin draining lymph nodes to prime adaptive immune cells, namely T- and B-lymphocytes, which will ultimately lead to a broad range of immune responses. Moreover, LCs have been shown to possess important roles in the anti-cancer immune responses. Indeed, the literature nicely highlights the role of LCs in melanoma. In line with this, LCs have been found in melanoma tissues where they contribute to the local immune response. Moreover, the immunogenic properties of LCs render them attractive targets for designing vaccines to treat melanoma and autoimmune diseases. Overall, future studies will help to enlarge the portfolio of immune properties of LCs, and aid the prognosis and development of novel therapeutic approaches to treating skin pathologies, including cancers.
Outcomes after Transcatheter Mitral Valve Implantation: A Literature Review
Authors: Samanidis George , Kanakis Meletios , Perreas Konstantinos
Published: December 2022

Mitral valve disease is the most common heart valve disease worldwide. Surgical mitral valve replacement or repair has been an established therapy in patients with severe mitral valve disease for many years. On the other hand, many patients with advanced mitral valve disease and severe comorbidities are treated conservatively and are excluded from the surgical procedure. Furthermore, in patients with severe comorbidities, transcatheter mitral valve repair by edge-to-edge technique with MitraClip or transcatheter mitral valve repair with a non-absorbable ring have been added as therapeutic options over the last few years. Alternative procedures for the treatment of patients with advanced prosthetic or native mitral valve diseases include transcatheter access for replacement or implantation of a new prosthetic valve in the diseased mitral valve. Promising results were published about short-term outcomes of patients who underwent the transcatheter mitral valve replacement. The current view and results of the transcatheter mitral valve implantation in patients with advanced native or prosthetic mitral valve disease are briefly discussed.
Tracking Changing Evidence of Water Erosion in Ordos Plateau, China Using the Google Earth Engine
Authors: Liu Yang , Liu Junhui , Zheng Yingjuan , Kang Yulin , Ma Su , Zhou Jianan
Published: December 2022

Ordos Plateau is one of the primary sources of sediment in the Yellow River, and changes in regional soil erosion directly affect the ecological status of the lower reaches of the Yellow River. Many recent studies have been published using remote sensing (RS) and geographic information systems (GIS) to evaluate soil erosion. In this study, much satellite remote sensing data in the Google Earth Engine (GEE) can better track soil erosion protection, which is significant in guiding the ecological protection and restoration of the Ordos Plateau and the Yellow River basin. In this study, we used GEE to observe the changes in soil erosion in the Ordos area from 2013 to 2021. The Theil–Sen procedure and Mann–Kendall significance test methods were used to evaluate the trend of land erosion in the Ordos area from 2013 to 2021. Based on GEE, the RUSLE is applied to evaluate soil erosion and analyze the changing trend. As a result, (1) we found that the annual change of soil and water loss in the Ordos Plateau showed three stages: 2013–2015, 2016–2018, and 2018–2021. After 2018, soil loss decreased from 14 × 1017 Mg in 2018 to 4 × 1017 Mg in 2021, which indicates that the environmental restoration project started in 2018 has achieved encouraging results. (2) The results showed that 40.9% of the regional soil erosion trend showed a significant decline, and 45.7% of the regional soil erosion trend showed a slight decline. Only 13.3% of the regional soil erosion is on the rise. (3) The test results of different land use types show that 87.3% of soil erosion occurs in bare and cultivated land. Because the terrain of Ordos is relatively flat, 95.39–96.17% of soil erosion occurs in areas with a slope of 0 to 5. (4) The reliability of the RUSLE model based on the GEE platform is proved by regression model verification of observation data and model prediction results. (5) GEE’s cloud-based features can provide data and scripts to users in developing countries which lack sufficient observation data or the necessary computing resources to develop these data. The results show that GEE has robust analysis and processing ability, can analyze a large amount of data, and can provide efficient digital products for soil erosion research.