bluuebunny/crossref_metadata_2025 huggingface dataset:
Business Methodology for the Application in University Environments of Predictive Machine Learning Models Based on an Ethical Taxonomy of the Student’s Digital Twin
Authors: Garay Gallastegui Luis Miguel , Reier Forradellas Ricardo Francisco
Published: October 2021

Educational institutions are undergoing an internal process of strategic transformation to adapt to the challenges caused by the growing impact of digitization and the continuous development of student and labor market expectations. Consequently, it is essential to obtain more accurate knowledge of students to improve their learning experience and their relationship with the educational institution, and in this way also contribute to evolving those students’ skills that will be useful in their next professional future. For this to happen, the entire academic community faces obstacles related to data capture, analysis, and subsequent activation. This article establishes a methodology to design, from a business point of view, the application in educational environments of predictive machine learning models based on Artificial Intelligence (AI), focusing on the student and their experience when interacting physically and emotionally with the educational ecosystem. This methodology focuses on the educational offer, relying on a taxonomy based on learning objects to automate the construction of analytical models. This methodology serves as a motivating backdrop to several challenges facing educational institutions, such as the exciting crossroads of data fusion and the ethics of data use. Our ultimate goal is to encourage education experts and practitioners to take full advantage of applying this methodology to make data-driven decisions without any preconceived bias due to the lack of contrasting information.
Digital Platform Ecosystems for Sustainable Innovation: Toward a New Meta-Organizational Model?
Authors: Calabrese Mario , La Sala Antonio , Fuller Ryan Patrick , Laudando Antonio
Published: October 2021

This work aims to develop a conceptual model to support countries, institutions, and firms toward the accomplishment of present Agenda 2030 sustainability goals. The last two decades have seen a growing interest in sustainability. Climate change, resource scarcity, multipolarity of interests, mistrust and delegitimization of institutions are just some of the critical issues that need to be addressed. There is broad consensus on the urgency of generating further social, environmental, and economic innovation to address these challenges, reshaping global markets, and offering new spaces of action to firms and institutions. Accordingly, there also is a wide search for new models of organizing firms. Digital platforms are among those. Moreover, since digital platforms require coordination among multiple actors and interests in order to succeed, they may also be conceptualized as meta-organizations, less hierarchical than firms yet more tightly coupled than markets. However, despite the wide literature on platforms, this organizational lens seems not to have been taken into the right consideration. This conceptual work aims to fill this gap, providing a framework that clarifies why and how a digital platform ecosystem could configure a sustainable meta-organizational model, and also providing the main steps to build it.
Antioxidant, Antifungal and Phytochemical Investigations of Capparis spinosa L.
Authors: Rajhi Ilhem , Hernandez-Ramos Fabio , Abderrabba Manef , Ben Dhia Med Taieb , Ayadi Sameh , Labidi Jalel
Published: October 2021

The antioxidant and antifungal activities of crude hydro-ethanolic extract from Capparis spinosa L. (Capparidaceae) leaves and their fractions, obtained by liquid-liquid extraction (LLE) using solvents with increasing polarity (hexane, diethyl ether, ethyl acetate, butanol, and water), were investigated. The crude extract and the obtained fractions were characterized by colorimetric analysis, pyrolysis-gas chromatography (GC)-mass spectroscopy (MS), Fourier Transform Infrared Spectroscopy, and their antioxidant and antifungal capacity were determined. It was observed that the ethyl acetate fraction was enriched in polyphenols, the butanol fraction resulted in purified from proteins and the residual aqueous fraction contains more hydrophobic compounds. The evaluation of the antioxidant activity revealed that the ethyl acetate fraction possesses an interesting capacity 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging with a percentage of inhibition of 84.02% at a concentration of 2 mg/mL and better ferric reducing antioxidant power (FRAP) 4.275 ± 0.011 mmol/g of dry sample than the other fractions tested. Regarding the antifungal activity, the diethyl ether fraction showed the highest activity against Aspergillus niger with 58.78% of inhibition. The results obtained in this work showed the relevance of the valorization of the leaves of Capparis spinosa L., given its richness in bioactive molecules can be regarded as a natural source of antioxidant and antifungal and may be considered in the future to replace synthetic preservatives in food, pharmaceutic products and cosmetic.
Combined and Singular Effects of Ethanolic Extract of Persian Shallot (Allium hirtifolium Boiss) and Synbiotic Biomin®IMBO on Growth Performance, Serum- and Mucus-Immune Parameters and Antioxidant Defense in Zebrafish (Danio rerio)
Authors: Ghafarifarsani Hamed , Hoseinifar Seyed Hossein , Talebi Maedeh , Yousefi Morteza , Van Doan Hien , Rufchaei Rudabeh , Paolucci Marina
Published: October 2021

This study was carried out to evaluate combined and singular effects of ethanolic extract of Persian shallot (Allium hirtifolium Boiss) and synbiotic Biomin®IMBO on growth performance, innate immune responses, and antioxidant defense in zebrafish (Danio rerio). Fish with initial weight of 151.90 ± 0.31 mg were allocated in 21 10-L glass aquariums. The experimental groups were as follows: T1, control (without any supplementation); T2, 1% synbiotic; T3, 3% synbiotic; T4, 1% Persian shallot (as a medical plant); T5, 3% Persian shallot; T6, 1% Persian shallot and 1% synbiotic; T7, 3% Persian shallot and 3% synbiotic. At the end of the experiment (60 days), all treatments significantly showed higher final weight (FW), weight gain (WG), WG (%), and specific growth rate (SGR) compared with the fish fed on control diet. Furthermore, both synbiotic Biomin®IMBO and Persian shallot significantly improved intestine immune parameters including lysozyme, alternative complement hemolytic activity (ACH50), total immunoglobulin (total Ig), and myeloperoxidase (MPO) of zebrafish compared to fish fed on control diet (p < 0.05). Also, in all experimental groups, hepatic catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) activities significantly increased compared to the control group. Whereas, the highest MDA level was observed in the control group compared to the treatments (p < 0.05). Moreover, skin mucus immune parameters of zebrafish have been noticeably improved with synbiotic Biomin®IMBO and Persian shallot compared to fish fed on the control diet (p < 0.05). The results indicate that synbiotic or Persian shallot supplemented diet could enhance the general health status of the zebrafish.
Characterization, Stress Response and Functional Analyses of Giant River Prawn (Macrobrachium rosenbergii) Glucose-Regulated Protein 78 (Mr-grp78) under Temperature Stress and during Aeromonas hydrophila Infection
Authors: Srisapoome Prapansak , Ju-Ngam Tanya , Wongpanya Ratree
Published: October 2021

The endoplasmic reticulum (ER) is an organelle important for several functions of cellular physiology. This study identified the giant river prawn’s glucose-regulated protein 78 (Mr-grp78), which is important for ER stress mechanisms. Nucleotide and amino acid analyses of Mr-grp78, as compared with other species, revealed the highest similarity scores with the grp78 genes of crustaceans. An expression analysis by quantitative RT-PCR indicated that Mr-grp78 was expressed in all tissues and presented its highest expression in the ovary (57.64 ± 2.39-fold), followed by the gills (42.25 ± 1.12), hindgut (37.15 ± 2.47), thoracic ganglia (28.55 ± 2.45) and hemocytes (28.45 ± 2.26). Expression analysis of Mr-grp78 mRNA levels under Aeromonas hydrophila induction and heat/cold-shock exposure was conducted in the gills, hepatopancreas and hemocytes. The expression levels of Mr-grp78 in these tissues were highly upregulated 12 h after bacterial infection. In contrast, under heat- and cold-shock conditions, the expression of Mr-grp78 was significantly suppressed in the gills at 24–96 h and in the hepatopancreas at 12 h (p < 0.05). A functional analysis via Mr-grp78 gene knockdown showed that Mr-grp78 transcription in the gills, hepatopancreas and muscle strongly decreased from 6 to 96 h. Furthermore, the silencing of this gene effectively increased the sensitivity of the tested prawns to heat- and pathogenic-bacterium-induced stress. The results of this study clearly demonstrate the significant functional roles of Mr-grp78 in response to both temperature and pathogen treatments.
Ethical Challenges Posed by Advanced Veterinary Care in Companion Animal Veterinary Practice
Authors: Quain Anne , Ward Michael P. , Mullan Siobhan
Published: October 2021

Advanced veterinary care (AVC) of companion animals may yield improved clinical outcomes, improved animal welfare, improved satisfaction of veterinary clients, improved satisfaction of veterinary team members, and increased practice profitability. However, it also raises ethical challenges. Yet, what counts as AVC is difficult to pinpoint due to continuing advancements. We discuss some of the challenges in defining advanced veterinary care (AVC), particularly in relation to a standard of care (SOC). We then review key ethical challenges associated with AVC that have been identified in the veterinary ethics literature, including poor quality of life, dysthanasia and caregiver burden, financial cost and accessibility of veterinary care, conflicts of interest, and the absence of ethical review for some patients undergoing AVC. We suggest some strategies to address these concerns, including prospective ethical review utilising ethical frameworks and decision-making tools, the setting of humane end points, the role of regulatory bodies in limiting acceptable procedures, and the normalisation of quality-of-life scoring. We also suggest a role for retrospective ethical review in the form of ethics rounds and clinical auditing. Our discussion reenforces the need for a spectrum of veterinary care for companion animals.
Impacts of Rumen Degradable or Undegradable Protein Supplementation with or without Salt on Nutrient Digestion, and VFA Concentrations
Authors: Manoukian Marley , DelCurto Timothy , Kluth Janessa , Carlisle Tanner , Davis Noah , Nack Makae , Wyffels Samuel , Scheaffer Abe , Van Emon Megan
Published: October 2021

The objectives of this study were to evaluate the effect of differences in protein type and delivery method on rumen dynamics and nutrient digestion. Cows were allotted to rumen degradable protein (RDP) or rumen undegradable protein (RUP) and self-fed (SF) salt-limited pressed blocks or hand-fed (HF) loose supplement, resulting in four dietary treatments. There was a delivery effect (p = 0.04) on neutral detergent fiber (NDF) intake, as the SF animals consumed more NDF than HF animals. The RDP-SF animals had greater NDF digestibility (p = 0.04) and water intake (p = 0.03) than the three other treatments. Supplement intake displayed a protein type effect (p = 0.03), as RDP-supplemented animals consumed more supplement on a g·kg body weight (BW)−1 d−1 basis than RUP animals. There was an effect of protein type (p = 0.02) and delivery method (p = 0.03) on fluid flow rate, with RUP and HF cows having greater liquid flow rates. Ruminal pH was lower (p < 0.01) in RDP-HF cows than RDP-SF cows at all hours, except 4-h post-feeding. RDP-SF animals had the greatest (p < 0.01) concentrations of ruminal ammonia. Valerate ruminal concentrations were greater (p = 0.04) in RDP supplemented animals compared to RUP supplemented animals. In conclusion, self-fed supplements containing RDP may enhance the use of low-quality forages and increase ruminal ammonia concentrations.
Insights into the Phytochemical and Multifunctional Biological Profile of Spices from the Genus Piper
Authors: Luca Simon Vlad , Gaweł-Bęben Katarzyna , Strzępek-Gomółka Marcelina , Czech Karolina , Trifan Adriana , Zengin Gokhan , Korona-Glowniak Izabela , Minceva Mirjana , Gertsch Jürg , Skalicka-Woźniak Krystyna
Published: October 2021

Piper spices represent an inexhaustible reservoir of bioactive compounds that may act as drug leads in natural product research. The aim of this study was to investigate a series of methanolic fruit extracts obtained from P. nigrum (black, green, white and red), P. longum and P. retrofractum in comparative phytochemical and multi-directional biological (antimicrobial, antioxidant, anti-enzymatic and anti-melanogenic) assays. The metabolite profiling revealed the presence of 17 piperamides, with a total content of 247.75–591.42 mg piperine equivalents/g. Among the 22 tested microorganism strains, Piper spices were significantly active (MIC < 0.1 mg/mL) against the anaerobes Actinomyces israelii and Fusobacterium nucleatum. The antioxidant and anti-enzymatic activities were evidenced in DPPH (10.64–82.44 mg TE/g) and ABTS (14.20–77.60 mg TE/g) radical scavenging, CUPRAC (39.94–140.52 mg TE/g), FRAP (16.05–77.00 mg TE/g), chelating (0–34.80 mg EDTAE/g), anti-acetylcholinesterase (0–2.27 mg GALAE/g), anti-butyrylcholinesterase (0.60–3.11 mg GALAE/g), anti-amylase (0.62–1.11 mmol ACAE/g) and anti-glucosidase (0–1.22 mmol ACAE/g) assays. Several Piper extracts (10 μg/mL) inhibited both melanin synthesis (to 32.05–60.65% of αMSH+ cells) and release (38.06–45.78% of αMSH+ cells) in αMSH-stimulated B16F10 cells, partly explained by their tyrosinase inhibitory properties. Our study uncovers differences between Piper spices and sheds light on their potential use as nutraceuticals or cosmeceuticals for the management of different diseases linked to bacterial infections, Alzheimer’s dementia, type 2 diabetes mellitus or hyperpigmentation.
Exploring the Impact of Flavonoids on Symptoms of Depression: A Systematic Review and Meta-Analysis
Authors: Ali Sawan , Corbi Graziamaria , Maes Michael , Scapagnini Giovanni , Davinelli Sergio
Published: October 2021

Recent evidence suggests that diet modifies key biological factors associated with the development of depression. It has been suggested that this could be due to the high flavonoid content commonly found in many plant foods, beverages and dietary supplements. Our aim was to conduct a systematic review to evaluate the effects of dietary flavonoids on the symptoms of depression. A total of 46 studies met the eligibility criteria. Of these, 36 were intervention trials and 10 were observational studies. A meta-analysis of 36 clinical trials involving a total of 2788 participants was performed. The results showed a statistically significant effect of flavonoids on depressive symptoms (mean difference = −1.65; 95% C.I., −2.54, −0.77; p < 0.01). Five of the 10 observational studies included in the systematic review reported significant results, suggesting that a higher flavonoid intake may improve symptoms of depression. Further studies are urgently required to elucidate whether causal and mechanistic links exist, along with substantiation of functional brain changes associated with flavonoid consumption.
Physical Ergonomics in Peripheral Nerve Block
Authors: Fathil Shahridan Mohd , Ghani Muhamad Rasydan Abdul , Chen Kung-Yen , Lee Pinhsin , Hou Jin-De , Lin Jui-An
Published: October 2021

The understanding of ergonomics is a vital competency for all peripheral nerve block operators. The essence of physical ergonomics for peripheral nerve block procedures can be summarised into three significant components: brain, musculoskeletal and needling. The first component includes strategies to optimise visuospatial neuroprocessing using equipment configuration. The second component reflects the careful planning of posture and position to improve procedural technique and reduce physical fatigue. The final component focuses on strategies to achieve needle beam alignment for optimal needle visualisation.
Evaluation of Bacterial Diversity and Quality Features of Traditional Sichuan Bacon from Different Geographical Region
Authors: Wang Song , Wang Xingjie , Pan Wanshu , Liu Aiping , Liu Shuliang , Yang Yong , Zou Likou
Published: October 2021

Sichuan bacon is one of the most popular types of Chinese bacon in the domestic market. High-throughput sequencing was used to characterize the bacterial diversity of 39 Sichuan bacon samples collected from 3 geographical regions. The results showed that the bacterial diversity of Sichuan bacon in different regions demonstrated certain specificity as well as similarity, and the shared OTUs were close to 81% of the total number in the basin group, mountain group, and plateau group. At the genus level, Staphylococcus is the most dominant genus among the three groups, covering 26.7%, 20.6%, and 22.7%, respectively. Beta diversity shows significant differences in bacterial compositions in different geographic regions, especially for Pseudomonas, Brochothrix, Lactobacillus, Lactococcus, and Enterococcus. Meanwhile, some physicochemical characteristics were analyzed, and a significant difference (p < 0.05) among the three regions was shown in the Aw, pH, and nitrite content, which were significantly correlated with undesired bacteria. This study provides insights into the understanding of the role of bacterial communities in the microbial safety and quality improvement of Sichuan bacon.
The Evolutions in Time of Probability Density Functions of Polydispersed Fuel Spray—The Continuous Mathematical Model
Authors: Hareli Shlomo , Nave Ophir , Gol’dshtein Vladimir
Published: October 2021

The dynamics of the particle size distribution (PSD) of polydispersed fuel spray is important in the evaluation of the combustion process. A better understanding of the dynamics can provide a tool for selecting a PSD that will more effectively meet the needs of the system. In this paper, we present an efficient and elegant method for evaluating the dynamics of the PSD. New insights into the behaviour of polydispersed fuel spray were obtained. A simplified theoretical model was applied to the experimental data and a known approximation of the polydispersed fuel spray. This model can be applied to any distribution, not necessarily an experimental distribution or approximation, and involves a time-dependent function of the PSD. Such simplified models are particularly helpful in qualitatively understanding the effects of various sub-processes. Our main results show that during the self-ignition process, the radii of the droplets decreased as expected, and the number of smaller droplets increased in inverse proportion to the radius. An important novel result (visualised by graphs) demonstrates that the mean radius of the droplets initially increases for a relatively short period of time, which is followed by the expected decrease. Our modified algorithm is superior to the well-known ‘parcel’ approach because it is much more compact; it permits analytical study because the right-hand sides of the mathematical model are smooth, and thus eliminates the need for a numerical algorithm to transition from one parcel to another. Moreover, the method can provide droplet radii resolution dynamics because it can use step functions that accurately describe the evolution of the radii of the droplets. The method explained herein can be applied to any approximation of the PSD, and involves a comparatively negligible computation time.
An Application of Safety Assessment for Radioactive Waste Repository: Non-Equilibrium Transport of Tritium, Selenium, and Cesium in Crushed Granite with Different Path Lengths
Authors: Lee Chuan-Pin , Chen Dongyang , Hu Yanqin , Jan Yi-Lin , Shi Yunfeng , Wang Ziteng , Wu Enhui , Tien Neng-Chuan , Sun Yuzhen , Tsai Shih-Chin
Published: October 2021

Advection-dispersion experiments (ADE) were effectively designed for inadequate transport models through a calibration/validation process. HTO, selenium (Se), and cesium (Cs) transport in crushed granite were studied using a highly reliable, dynamic column device in order to obtain the retardation factors (R) and the dispersion coefficients (D) by fitting experimental breakthrough curves (BTCs) for various path lengths. In order to conduct a safety assessment (SA) of a deep geological repository for high-level radioactive waste, radionuclide transport in rock systems is necessary to clarify and establish a suitable model. A dynamic column with a radiotracer (HTO, Se(IV), and Cs) was applied to 2, 4, and 8 cm path lengths using a STANMOD simulation. The results showed similar results between the BTCs of Se and Cs by fitting a non-equilibrium sorption model due to the retardation effect. In fact, there was a relatively obvious sorption of Se and Cs in the BTCs obtained by fitting a retardation factor (R) value higher than 1. In addition, a two-region (physical) and a two-site (chemical) non-equilibrium model with either the lowest sum of squared residuals (SSQ) or the root mean square error (RMSE) were applied to determine the Se and Cs sorption mechanisms on granite.
Special Issue “Novel Approaches and Applications in Ergonomic Design”
Authors: You Heecheon
Published: October 2021

Interactions between humans and systems need to be designed appropriately for safety, usability, productivity, health, and/or wellness [...]
Blue Whiting Protein Hydrolysates Exhibit Antioxidant and Immunomodulatory Activities in Stimulated Murine RAW264.7 Cells
Authors: Heffernan Shauna , Harnedy-Rothwell Pádraigín A. , Gite Snehal , Whooley Jason , Giblin Linda , Fitzgerald Richard J. , O’Brien Nora M.
Published: October 2021

This study investigated the antioxidant and immunomodulatory potential of six blue whiting soluble protein hydrolysates (BWSPHs, BW-SPH-A to -F) and their simulated gastrointestinal digests (SGID, BW-SPH-A-GI to -F-GI) in murine RAW264.7 macrophages. Hydrolysate BW-SPH-A, both pre- and post-SGID, increased endogenous antioxidant glutathione (GSH) in tert-butylhydroperoxide (tBOOH)-treated cells and reduced reactive oxygen species (ROS) in H2O2-challenged RAW264.7 cells compared with treated controls in the absence of BWSPHs (p < 0.05). BW-SPH-A-GI also exhibited higher ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) activities than the other BWSPHs tested (p < 0.05). All BWSPHs and SGID BWSPH samples induced immunostimulating effects in lipopolysaccharide (LPS)-activated RAW264.7 macrophages through the upregulation of NO production. BW-SPH-F-GI increased IL-6 and TNF-α levels compared with the LPS controls indicating the liberation of immunomodulatory peptide/amino acids during the SGID process. Therefore, BW-SPH-A and BW-SPH-F may have potential use against oxidative stress and immunosuppression-related diseases, respectively.
Applying Precision Agriculture to Artificial Waterfowl Hatching, Using the Black Muscovy Duck as an Example
Authors: Chang Shun-Chieh , Cheng Chih-Hsiang , Chen Yen-Hung
Published: October 2021

(1) Background: agriculture practices adopt homogenization-farming processes to enhance product characteristics, with lower costs, standardization, mass production, and production efficiency. (2) Problem: conventional agriculture practices eliminate products when these products are slightly different from the expected status in each phase of the lifecycle due to the changing natural environment and climate. However, this elimination of products can be avoided when they receive customized care to the expected developing path via a universal prediction model, for the quantitative description of biomass changing with time and the environment, and the corresponding automatic environmental controls. (3) Methods: in this study, we built a prediction model to quantitatively predict the hatching rate of each egg by observing the biomass development path along the waterfowl-like production lifecycle and the corresponding environment settings. (4) Results: two experiments using black Muscovy duck hatching as a case study were executed. The first experiment involved finding out the key characteristics, out of 25 characteristics, and building a prediction model to quantitatively predict the survivability of the black Muscovy duck egg. The second experiment was adopted to validate the effectiveness of our prediction mode; the hatching rate rose from 47% in the first experiment to 62% in the second experiment without any human interference from experienced farmers. (5) Contributions: this research builds on an AI-based precision agriculture system prototype as the reference for waterfowl research. The results show that our proposed model is capable of decreasing the training costs and enhancing the product qualification rate for individual agricultural products.
Dynamic Stability of an Electric Monowheel System Using LQG-Based Adaptive Control
Authors: Sengupta Ipsita , Gupta Sagar , Deb Dipankar , Ozana Stepan
Published: October 2021

This paper presents the simulation and calculation-based aspect of constructing a dynamically stable, self-balancing electric monowheel from first principles. It further goes on to formulate a reference model-based adaptive control structure in order to maintain balance as well as the desired output. First, a mathematical model of the nonlinear system analyzes the vehicle dynamics, followed by an appropriate linearization technique. Suitable parameters for real-time vehicle design are calculated based on specific constraints followed by a proper motor selection. Various control methods are tested and implemented on the state-space model of this system. Initially, classical pole placement control is carried out in MATLAB to observe the responses. The LQR control method is also implemented in MATLAB and Simulink, demonstrating the dynamic stability and self-balancing system property. Subsequently, the system considers an extensive range of rider masses and external disturbances by introducing white noise. The parameter estimation of rider position has been implemented using Kalman Filter estimation, followed by developing an LQG controller for the system, in order to mitigate the disturbances caused by factors such as wind. A comparison between LQR and LQG controllers has been conducted. Finally, a reference model-assisted adaptive control structure has been established for the system to account for sudden parameter changes such as rider mass. A reference model stabilizer has been established for the same purpose, and all results have been obtained by running simulations on MATLAB Simulink.
Observing Brief and Irregular Behaviour of Animals—The Validity of Short Observation Periods
Authors: Wilder Thore , Krieter Joachim , Kemper Nicole , Büttner Kathrin
Published: October 2021

There are efficient sampling methods to accurately estimate behaviour with a moderate or long duration. For short behaviour, observing animals continuously is recommended although there is no recommended minimum observation time. In most studies, sampling method and observation time per day is determined by practical considerations. Thus, this study analysed the validity of behavioural observations in different observation periods using continuous sampling (CS) or time sampling (TS) based on biting behaviour. Tail-biting and ear-biting of weaned piglets in six pens were continuously observed for 12 h per day for 4 days to form a reference. Shorter observation periods of CS and TS were simulated by taking subsets of this reference. The amount of behaviour per hour of each observation period was compared to the reference and to other observation period of the same kind and length. Four different measurements were defined to calculate accuracy scores (AS; 0–1; higher values are better). Comparison to the reference shows better AS for observation periods with longer observation time in total (0.5 h of CS: 0.2; 6 h of CS: 0.6). Additionally, TS covers longer time periods without decreasing AS. However, focus on activity time results in an overestimation of irregular behaviour. Comparing AS among observation periods of the same kind and length show overall low agreement. This study indicated problems of different observation periods of CS and TS to accurately estimate behaviour. Therefore, validity of behavioural observations should be analysed in greater detail to determine optimal sampling methods.
Design of Gas Cyclone Using Hybrid Particle Swarm Optimization Algorithm
Authors: Shen Xueli , Ihenacho Daniel C.
Published: October 2021

The method of searching for an optimal solution inspired by nature is referred to as particle swarm optimization. Differential evolution is a simple but effective EA for global optimization since it has demonstrated strong convergence qualities and is relatively straightforward to comprehend. The primary concerns of design engineers are that the traditional technique used in the design process of a gas cyclone utilizes complex mathematical formulas and a sensitivity approach to obtain relevant optimal design parameters. The motivation of this research effort is based on the desire to simplify complex mathematical models and the sensitivity approach for gas cyclone design with the use of an objective function, which is of the minimization type. The process makes use of the initial population generated by the DE algorithm, and the stopping criterion of DE is set as the fitness value. When the fitness value is not less than the current global best, the DE population is taken over by PSO. For each iteration, the new velocity and position are updated in every generation until the optimal solution is achieved. When using PSO independently, the adoption of a hybridised particle swarm optimization method for the design of an optimum gas cyclone produced better results, with an overall efficiency of 0.70, and with a low cost at the rate of 230 cost/s.
First Perceptions of Hydroperiod Mapping and Assessment of Shallow Waters in Coastal Landscapes by Drone-Based Monitoring Activities: A Remote-Sensing and GIS Approach
Authors: Scalici Massimiliano , Perrone Michela , Battisti Jacopo , Benedini Livia , Malavasi Marco
Published: October 2021

Beyond the importance of ponds for aquatic and terrestrial life, pond networks seem to be crucial to providing a vital spatial resource in response to global climate change for all migrating and spreading taxa. Additionally, ponds offer sustainable solutions to issues of concern in water management, such as nutrient retention, rainfall interception, or carbon sequestration. Although the ecological role of shallow waters seems clear, significant work must be performed to set future guidelines and actions towards their conservation. The main aims of the present study are to (i) georeference all small temporary wetlands within the Tyrrhenian central Italy coastal area, (ii) evaluate their hydroperiod, and (iii) calculate their surface size variability. We found 137 wetlands, 53 of which were temporary and contained listed habitats. Each wetland’s status was assessed in relation to land use and proximity to stressors (e.g., urban centres, railways, roads) while observing the relationship between pond occurrence, lithology, and permeability. Amongst the detected wetlands, we selected and monitored 21 temporary ponds (homogeneously distributed within the study area) for 12 months using images collected by the non-professional drone Parrot Bebop 2. All images were then acquired in ArcGIS to georeference all temporary ponds. The analysis confirmed that the majority of the surveyed ponds are in close proximity to roads and tracks, which might have significant impacts on the preservation of such fragile habitats. Moreover, despite the wide variability of hydroperiod duration, the greater part of the pools fill with water in autumn and dry in summer, in alignment with the Mediterranean climate. This preliminary study allowed for the creation of the first temporary ponds’ database, which is useful for monitoring their status in central Italy and planning further studies to assess eventual detrimental effects caused by human-mediated activities.
Hydrogen Peroxide Gas Plasma Sterilizer Combined with Dielectric Barrier Discharge and Corona Discharge Inactivates Prions
Authors: Sakudo Akikazu , Tsuji Yosuke
Published: October 2021

Prions are highly resistant to physical or chemical damage, although previous studies have shown that STERRAD®, a hydrogen gas plasma sterilizer using radiofrequency (RF) discharge, has an inactivation effect. Here, the effect of hydrogen peroxide gas combined with dielectric barrier discharge (DBD) plasma and corona discharge plasma using a RENO-S130 sterilizer on scrapie prions was examined. Scrapie prion-infected mouse brain homogenate was air-dried on a cover glass, sealed in a Tyvek pouch, and subjected to RENO-S130 treatment using either non-lumen mode (28 min) or Eco mode (45 min) with hydrogen peroxide gas derived from 50% hydrogen peroxide. Control (untreated) samples were prepared on a cover glass using the same procedure but without exposure to RENO-S130. PrPres (proteinase K (PK)-resistant prion protein), an index of the conformational variant of prion protein (PrPSc), was decreased by treatment with RENO-S130 under both modes of operation. Specifically, PrPres was identified after the 1st and 2nd cycles of protein misfolding cyclic amplification (PMCA) in control samples but was below the detection limit in RENO-S130-treated samples. A bioassay showed that treatment of prions with RENO-S130 (non-lumen or Eco mode) significantly prolonged mouse survival time. Taken together, these findings show hydrogen peroxide gas combined with DBD/corona discharge plasma can inactivate prions by reducing prion propagation and prion infectivity. This treatment is potentially applicable to the sterilization of prion-contaminated heat-sensitive medical devices.
Applications of Bioelectrical Impedance Vector Analysis (BIVA) in the Study of Body Composition in Athletes
Authors: de la Cruz Marcos Sandra , Redondo del Río Mª Paz , de Mateo Silleras Beatriz
Published: October 2021

The study of body composition (BC) allows evaluating nutritional status, optimizing competitive performance, and monitoring the success of training regimens in athletes. Since BC reference techniques are expensive and/or invasive, in practice simpler, less expensive, and safer methods such as anthropometry are often used. The problem is that anthropometry requires a qualified anthropometrist, a rigorous measurement protocol using validated equipment, and a lengthy procedure because of its limited accuracy. Bioimpedance analysis (BIA) is an indirect method for estimating BC in a simple, fast, inexpensive, noninvasive, accurate, and reproducible manner. Despite the limitations of conventional BIA, it is still the most commonly used method for assessing BC in athletes. However, bioelectrical impedance vector analysis (BIVA) allows qualitative assessment of body cell mass (BCM) and hydration status without the need for biological assumptions and without being subject to conventional BIA errors. The purpose of this article was to demonstrate the uses and applications of BIA in the study of the BC and physical characteristics of athletes. For example, several practical cases of athletes from different sports were compared. The results obtained from somatotype studies, conventional BIA, and BIVA were included. BIVA interpretation and somatotype were consistent for all cases analyzed, but BIVA also provided information about hydration and BCM. The application of BIVA in the assessment of athletes can improve BC studies in sports because it is fast, simple, and reliable and neither imposes two-compartment-model limitations on BC estimation nor requires any biological assumptions.
Drought Stress during Anthesis Alters Grain Protein Composition and Improves Bread Quality in Field-Grown Iranian and German Wheat Genotypes
Authors: Rekowski Azin , Wimmer Monika A. , Tahmasebi Sirous , Dier Markus , Kalmbach Sarah , Hitzmann Bernd , Zörb Christian
Published: October 2021

Drought stress is playing an increasingly important role in crop production due to climate change. To investigate the effects of drought stress on protein quantity and quality of wheat, two Iranian (Alvand, Mihan) and four German (Impression, Discus, Rumor, Hybery) winter wheat genotypes, representing different quality classes and grain protein levels, were grown under field conditions in Eqlid (Iran) during the 2018–2019 growing season. Drought stress was initiated by interrupting field irrigation during the anthesis phase at two different stress levels. Drought stress at anthesis did not significantly change total grain protein concentration in any of the wheat genotypes. Similarly, concentrations of grain storage protein sub-fractions of albumin/globulin, gliadin and glutenin were unaltered in five of the six genotypes. However, analysis of protein sub-fractions by SDS polyacrylamide gel electrophoresis revealed a consistent significant increase in ω-gliadins with increasing drought stress. Higher levels of HMW glutenins and a reduction in LMW-C glutenins were observed exclusively under severe drought stress in German genotypes. The drought-induced compositional change correlated positively with the specific bread volume, and was mainly associated with an increase in ω-gliadins and with a slight increase in HMW glutenins. Despite the generally lower HMW glutenin concentrations of the Iranian genotypes and no effect of drought on the concentration of HMW sub-fraction, there was still high specific bread volume under drought. It is suggested that for the development of new wheat cultivars adapted to these challenging climatic conditions, the protein composition should be considered in addition to the yield and grain protein concentration.
Complex Attosecond Waveform Synthesis at FEL FERMI
Authors: Maroju Praveen Kumar , Grazioli Cesare , Di Fraia Michele , Moioli Matteo , Ertel Dominik , Ahmadi Hamed , Plekan Oksana , Finetti Paola , Allaria Enrico , Giannessi Luca , De Ninno Giovanni , Lutman Alberto A. , Squibb Richard J. , Feifel Raimund , Carpeggiani Paolo , Reduzzi Maurizio , Mazza Tommaso , Meyer Michael , Bengtsson Samuel , Ibrakovic Neven , Simpson Emma Rose , Mauritsson Johan , Csizmadia Tamás , Dumergue Mathieu , Kühn Sergei , Gopalakrishnan Harshitha Nandiga , You Daehyun , Ueda Kiyoshi , Labeye Marie , Bækhøj Jens Egebjerg , Schafer Kenneth J. , Gryzlova Elena V. , Grum-Grzhimailo Alexei N. , Prince Kevin C. , Callegari Carlo , Sansone Giuseppe
Published: October 2021

Free-electron lasers (FELs) can produce radiation in the short wavelength range extending from the extreme ultraviolet (XUV) to the X-rays with a few to a few tens of femtoseconds pulse duration. These facilities have enabled significant breakthroughs in the field of atomic, molecular, and optical physics, implementing different schemes based on two-color photoionization mechanisms. In this article, we present the generation of attosecond pulse trains (APTs) at the seeded FEL FERMI using the beating of multiple phase-locked harmonics. We demonstrate the complex attosecond waveform shaping of the generated APTs, exploiting the ability to manipulate independently the amplitudes and the phases of the harmonics. The described generalized attosecond waveform synthesis technique with an arbitrary number of phase-locked harmonics will allow the generation of sub-100 as pulses with programmable electric fields.
Polyurethane Treated in Ar/C2H2/Ar Plasma: Towards Deformable Coating with Improved Albumin Adsorption
Authors: Morozov Ilya A. , Kamenetskikh Alexander S. , Beliaev Anton Y. , Izumov Roman I. , Scherban Marina G. , Lemkina Larisa M. , Kiselkov Dmitriy M.
Published: October 2021

Plasma modification of soft polymeric surfaces has many prospects in creating biomedical devices. The deformability of the obtained coatings should be studied, as the usage of such materials implies mechanical loads. Polyurethane (a two-phase synthetic polymer) treated in argon/acetylene plasma, with post-treatment in argon plasma, was investigated. A carbon-containing nanocoating (discontinuous mesh-like structures) with structural–mechanical inhomogeneities is formed by the action of Ar/C2H2 plasma. The heterogeneities of the coating are due to the complex structure of the initial substrate and short duration of treatment; as the treatment time increases, the coatings become homogeneous, but their stiffness rises. The treated surfaces in the uniaxial tensile state have micro and/or nanocracks in certain cases of plasma treatment. This is associated with an increased elastic modulus of the coatings. The coatings without cracks have regions with sufficiently alternating stiffness. Post-treatment in argon plasma increases wettability and free surface energy, positively affecting the adsorption of albumin. The stiffness of such coatings increases, becoming more homogeneous, which slightly reduces their crack resistance. Thus, plasma coatings on soft polymers operating under mechanical loads without causing damage should have sufficiently low stiffness, and/or structural-mechanical heterogeneities that provide redistribution of stress.
Impacts of the COVID-19 Lockdown Measures on the 2020 Columnar and Surface Air Pollution Parameters over South-Eastern Italy
Authors: Romano Salvatore , Catanzaro Valentina , Paladini Fabio
Published: October 2021

The combined use of Lecce-University AERONET-photometer measurements and PM2.5, PM10, NO2, CO, and SO2 concentrations from different sites of Apulia-Region Air-Quality Agency represents the peculiarity of this study, which evaluates the impact of COVID-19 lockdown (LD) measures on aerosol and gaseous pollutants. Monthly-averaged columnar and surface parameters of the 2020-year were compared with corresponding monthly parameters of the ref-year obtained by averaging 2017, 2018, and 2019 measurements in order to evaluate LD measure impacts by Average Percent Departure (APD%). Photometer measurements showed that LD measures were likely responsible for the decrease in Aerosol Optical Depth (AOD). The APD% estimated between the 2020- and ref-year AOD (at 440 nm) was characterized by negative values from June to August, reaching the smallest mean value (−46%) in June. Moreover, the columnar aerosol load appeared less affected by continental urban/industrial particles than previous years in the summer of 2020. The PM-concentration-APD% calculated at ten sites was characterized by monthly trends similar to those of AOD-APD%. PM-APD% values varied from site to site and smaller values (up to −57% in June) were on average detected at urban/suburban sites than at background sites (up to −37%). The impact of LD measures on gaseous pollutants was observed from the onset of LD.
Surface Urban Heat Island Assessment of a Cold Desert City: A Case Study over the Isfahan Metropolitan Area of Iran
Authors: Karimi Alireza , Mohammad Pir , Gachkar Sadaf , Gachkar Darya , García-Martínez Antonio , Moreno-Rangel David , Brown Robert D.
Published: October 2021

This study investigates the diurnal, seasonal, monthly and temporal variation of land surface temperature (LST) and surface urban heat island intensity (SUHII) over the Isfahan metropolitan area, Iran, during 2003–2019 using MODIS data. It also examines the driving factors of SUHII like cropland, built-up areas (BI), the urban–rural difference in enhanced vegetation index (ΔEVI), evapotranspiration (ΔET), and white sky albedo (ΔWSA). The results reveal the presence of urban cool islands during the daytime and urban heat islands at night. The maximum SUHII was observed at 22:30 p.m., while the minimum was at 10:30 a.m. The summer months (June to September) show higher SUHII compared to the winter months (February to May). The daytime SUHII demonstrates a robust positive correlation with cropland and ΔWSA, and a negative correlation with ΔET, ΔEVI, and BI. The nighttime SUHII displays a negative correlation with ΔET and ΔEVI.
An Application of the LCZ Approach in Surface Urban Heat Island Mapping in Sofia, Bulgaria
Authors: Dimitrov Stelian , Popov Anton , Iliev Martin
Published: October 2021

This article presents the results of the thermal survey of the capital of Bulgaria (Sofia) carried out in August 2019, with the application of an unmanned aerial system (UAS). The study is based on the concept of local climate zones (LCZs), taking into account the influence of the features of land use/land cover and urban morphology on the urban climate. The basic spatial units used in the study are presented in the form of a regular grid consisting of 3299 cells with sides of 250 × 250 m. A total of 13 types of LCZs were identified, of which LCZs 6, 5, 8, 4, D, and A form the largest share. In the thermal imaging of the surface, a stratified sampling scheme was applied, which allowed us to select 74 cells, which are interpreted as representative of all cells belonging to the corresponding LCZ in the urban space. The performed statistical analysis of the thermal data allowed us to identify both the most thermally loaded zones (LCZs 9, 4, and 5) and the cells forming Urban Cool Islands (mainly in LCZs D and C). The average surface temperature in Sofia during the study period (in the time interval between 8:00 p.m. and 10:00 p.m.) was estimated at 20.9 °C, and between the different zones it varied in the range 17.2–25.1 °C. The highest maximum values of LST (27.9–30.6 °C) were registered in LCZ 4 and LCZ 5. The relation between the spatial structure of the urban thermal patterns and urban surface characteristics was also analyzed. Regression analysis confirmed the hypothesis that as the proportion of green areas increases, surface temperatures decrease, and, vice versa, as the proportion of built-up and impermeable areas increases, surface temperatures increase. A heat load map (via applying a z-transformation to standardize the temperature values), a map of the average surface temperature, and a map of the average intensity of the heat island on the surface were generated in the GIS environment. The results of the study adequately reflect the complex spatial model of the studied phenomenon, which gives grounds to conclude that the research approach used is applicable to similar studies in other cities.