bluuebunny/crossref_metadata_2025 huggingface dataset:
Photobiologische Wirkungen der Sonnenstrahlung auf die Haut und Schutz durch Sonnenschutzmittel
Authors: Kockott D. , Herzog B.
Published: May 2018

Die Wellenlängenabhängigkeit photobiologischer Wirkungen wird durch Wirkungsspektren beschrieben. Die physikalischen Voraussetzungen für die Ermittlung und Anwendung von Wirkungsspektren werden erläutert. Die Bewertung von Strahlungsquellen durch Wirkungsspektren wird am Beispiel der Erythembildung und der Bildung von Provitamin D gezeigt.

Die Schutzwirkung von Sonnenschutzmitteln in verschiedenen Spektralbereichen kann durch die Wahl geeigneter UV-Absorber variiert werden. Die Bestimmung von Schutzfaktoren gegenüber erythemwirksamer Strahlung und UVA-Strahlung wird beschrieben. Sonnenschutzmittel reduzieren automatisch auch diejenigen Strahlungsanteile, die für die Produktion von Provitamin D verantwortlich sind. Es wird eine Optimierung von Sonnenschutzmitteln beschrieben, die bei vorgegebenem Erythemschutz ein Optimum an Provitamin D-erzeugender Strahlung auf der Haut zulassen.
Xeroderma pigmentosum – Fakten und Perspektiven
Authors: Lehmann J. , Seebode C. , Martens M. , Emmert S.
Published: May 2018

Die Nukleotid-Exzisions-Reparatur (NER) ist für die Beseitigung von ultraviolett (UV) -induzierten DNA-Schäden und damit zur Vermeidung von Hautkrebs essenziell. Menschen mit einem genetischen Defekt in der NER, Xeroderma pigmentosum (XP) -Patienten, sind äußerst sonnenempfindlich. Sie entwickeln bereits in den ersten Lebensjahren Zeichen der vorzeitigen Hautalterung mit einem deutlich erhöhten Risiko zur Entwicklung von UV-induzierten kutanen Karzinomen. DNA-Reparaturdefektsyndrome werden vorrangig in der Klinik diagnostiziert und auf molekularer Ebene bestätigt. Für die seltene, rezessiv vererbte Erkrankung XP steht zum jetzigen Zeitpunkt leider noch keine kausale Therapie zur Verfügung, weshalb eine frühe Diagnosestellung umso bedeutsamer ist. Durch frühzeitige sowie konsequente UV-protektive Maßnahmen und eine regelmäßige Überprüfung der Haut im Zuge der Hautkrebsfrüherkennung werden sowohl die Prognose als auch Krankheitsverlauf maßgeblich verbessert.
Design and experimental results of the 1-T Bitter Electromagnet Testing Apparatus (BETA)
Authors: Bates E. M. , Birmingham W. J. , Romero-Talamás C. A.
Published: May 2018

The Bitter Electromagnet Testing Apparatus (BETA) is a 1-Tesla (T) technical prototype of the 10 T Adjustable Long Pulsed High-Field Apparatus. BETA’s final design specifications are highlighted in this paper which include electromagnetic, thermal, and stress analyses. We discuss here the design and fabrication of BETA’s core, vessel, cooling, and electrical subsystems. The electrical system of BETA is composed of a scalable solid-state DC breaker circuit. Experimental results display the stable operation of BETA at 1 T. These results are compared to both analytical design and finite element calculations. Experimental results validate analytical magnet designing methods developed at the Dusty Plasma Laboratory. The theoretical steady state maxima and the limits of BETA’s design are explored in this paper.
Rapid surface defect detection based on singular value decomposition using steel strips as an example
Authors: Sun Qianlai , Wang Yin , Sun Zhiyi
Published: May 2018

For most surface defect detection methods based on image processing, image segmentation is a prerequisite for determining and locating the defect. In our previous work, a method based on singular value decomposition (SVD) was used to determine and approximately locate surface defects on steel strips without image segmentation. For the SVD-based method, the image to be inspected was projected onto its first left and right singular vectors respectively. If there were defects in the image, there would be sharp changes in the projections. Then the defects may be determined and located according sharp changes in the projections of each image to be inspected. This method was simple and practical but the SVD should be performed for each image to be inspected. Owing to the high time complexity of SVD itself, it did not have a significant advantage in terms of time consumption over image segmentation-based methods. Here, we present an improved SVD-based method. In the improved method, a defect-free image is considered as the reference image which is acquired under the same environment as the image to be inspected. The singular vectors of each image to be inspected are replaced by the singular vectors of the reference image, and SVD is performed only once for the reference image off-line before detecting of the defects, thus greatly reducing the time required. The improved method is more conducive to real-time defect detection. Experimental results confirm its validity.
Analysis of disturbances in a hypersonic boundary layer on a cone with heating/cooling of the nose tip
Authors: Bountin Dmitry , Maslov Anatoly , Gromyko Yury
Published: May 2018

Experimental results of the influence of local heating/cooling on the development of hypersonic boundary layer disturbances are reported. Local heating/cooling is applied at the cone nose tip. The experiments are carried out at the Mach number M = 5.95, stagnation temperature T0 = 360–418 K, and stagnation pressure P0 = 3.7–45 atm. The unit Reynolds number is varied in the interval Re1 = (4.5–63) × 106 m−1. The investigations are conducted in the boundary layer on a cone with an apex half-angle of 7° and varied bluntness radius of the nose tip [R = 0.03 (sharp nose), 0.75, and 1.5 mm] for different values of the local temperature factor. The nose tip is heated by an ohmic heater. Cooling is performed by supplying liquid nitrogen into the internal cavity of the model nose. A comparative analysis of pressure pulsation spectra on the cone surface is performed. It is demonstrated that heating/cooling in the case of a sharp cone leads to flow destabilization/stabilization. The opposite effect is observed for blunted cones: heating/cooling stabilizes/destabilizes the second-mode disturbances. This effect is enhanced by increasing the nose tip bluntness. All the observed effects vanish with distance downstream from the nose tip.
A fibre-coupled UHV-compatible variable angle reflection-absorption UV/visible spectrometer
Authors: Stubbing J. W. , Salter T. L. , Brown W. A. , Taj S. , McCoustra M. R. S.
Published: May 2018

We present a novel UV/visible reflection-absorption spectrometer for determining the refractive index, n, and thicknesses, d, of ice films. Knowledge of the refractive index of these films is of particular relevance to the astrochemical community, where they can be used to model radiative transfer and spectra of various regions of space. In order to make these models more accurate, values of n need to be recorded under astronomically relevant conditions, that is, under ultra-high vacuum (UHV) and cryogenic cooling. Several design considerations were taken into account to allow UHV compatibility combined with ease of use. The key design feature is a stainless steel rhombus coupled to an external linear drive (z-shift) allowing a variable reflection geometry to be achieved, which is necessary for our analysis. Test data for amorphous benzene ice are presented as a proof of concept, the film thickness, d, was found to vary linearly with surface exposure, and a value for n of 1.43 ± 0.07 was determined.
Vertical sizes of 1-D and 2-D electrostatic solitons with nonextensive and trapped electrons in the upper ionosphere
Authors: Ali Shan Shaukat , Saleem Hamid
Published: May 2018

The vertical sizes of one-dimensional (1-D) and two dimensional (2-D) electrostatic solitons are estimated in the oxygen-hydrogen (O – H) and pure oxygen plasmas of the upper ionosphere taking into account the effects of non-extensive and trapped electrons. The field-aligned flow of oxygen ions is also considered. It is found that both electron trapping and non-extensivity play a constructive role in the formation of 1-D and 2-D solitary structures. The vertical size of the solitons is not known through observations, but here it is pointed out that the vertical size of these structures should be of the order of a few meters at the altitude of 800 km in the 1-D case. On the other hand, in the 2-D case, the vertical size is much larger than the horizontal size and it turns out to be of the order of a few kilometers, while the width is about a few hundred meters in agreement with the observations.
Shed vortex structure and phase-averaged velocity statistics in symmetric/asymmetric turbulent flat plate wakes
Authors: Rai Man Mohan
Published: May 2018

The near wake of a flat plate is investigated via direct numerical simulations. Many earlier experimental investigations have used thin plates with sharp trailing edges and turbulent boundary layers to create the wake. This results in large θ/DTE values (θ is the boundary layer momentum thickness toward the end of the plate and DTE is the trailing edge thickness). In the present study, the emphasis is on relatively thick plates with circular trailing edges (CTEs) resulting in θ/D values less than one (D is the plate thickness and the diameter of the CTE) and vigorous vortex shedding. The Reynolds numbers based on the plate length and D are 1.255 × 106 and 10 000, respectively. Two cases are computed: one with turbulent boundary layers on both the upper and lower surfaces of the plate (statistically the same, symmetric wake, Case TT) and the other with turbulent and laminar boundary layers on the upper and lower surfaces, respectively (asymmetric case, Case TL). The data and understanding obtained are of considerable engineering interest, particularly in turbomachinery where the pressure side of an airfoil can remain laminar or transitional because of a favorable pressure gradient and the suction side is turbulent. Shed-vortex structure and phase-averaged velocity statistics obtained in the two cases are compared here. The upper negative shed vortices in Case TL (turbulent separating boundary layer) are weaker than the lower positive ones (laminar separating boundary layer) at inception (a factor of 1.27 weaker in terms of peak phase-averaged spanwise vorticity at the first appearance of a peak). The upper vortices weaken rapidly as they travel downstream. A second feature of interest in Case TL is a considerable increase in the peak phase-averaged, streamwise normal intensity (random component) with increasing streamwise distance (x/D) that occurs near the positive vortex cores. This behavior is observed for a few diameters in the near wake. This is counter to Case TT where the peak value essentially decreases with increasing x/D. Both these effects are examined in detail, and the important contributors are identified.
On boundaries of ping-pong modes in multipacting
Authors: Shemelin Valery
Published: May 2018

Multipactor is an avalanche multiplication of the number of electrons in radio-frequency devices due to secondary electron emission. One of the possible modes of this kind of discharge was studied in detail and was named “a ping-pong mode” in a publication by R. A. Kishek [Phys. Rev. Lett. 108, 035003 (2012) and Phys. Plasmas 20, 056702 (2013)]. In the present paper, I show that conditions of stability and cutoff limits are quite different from those derived in the cited papers.
Orientation dependent cyclic stability of the elastocaloric effect in textured Ni-Mn-Ga alloys
Authors: Wei Longsha , Zhang Xuexi , Liu Jian , Geng Lin
Published: May 2018

High-performance elastocaloric materials require a large reversible elastocaloric effect and long life cyclic stability. Here, we fabricated textured polycrystalline Ni50.4Mn27.3Ga22.3 alloys by cost-effective casting method to create a <001> texture. A strong correlation between the cyclic stability and the crystal orientation was demonstrated. A large reversible adiabatic temperature change ΔT ∼6 K was obtained when the external stress was applied parallel to <001> direction. However, the ΔT decreased rapidly after 50 cycles, showing an unstable elastocaloric effect (eCE). On the other hand, when the external stress was applied perpendicular to <001>, the adiabatic ΔT was smaller ∼4 K, but was stable over 100 cycles. This significantly enhanced eCE stability was related to the high yield strength, low transformation strain and much higher crack initiation-propagation resistances perpendicular to <001> direction. This study provides a feasible strategy for optimizing the eCE property by creation of the texture structure in polycrystalline Ni-Mn-Ga and Ni-Mn-X (X= In, Sn, Sb) alloys.
Excitation of higher radial modes of azimuthal surface waves in the electron cyclotron frequency range by rotating relativistic flow of electrons in cylindrical waveguides partially filled by plasmas
Authors: Girka Igor O. , Pavlenko Ivan V. , Thumm Manfred
Published: May 2018

Azimuthal surface waves are electromagnetic eigenwaves of cylindrical plasma-dielectric waveguides which propagate azimuthally nearby the plasma-dielectric interface across an axial external stationary magnetic field. Their eigenfrequency in particular can belong to the electron cyclotron frequency range. Excitation of azimuthal surface waves by rotating relativistic electron flows was studied in detail recently in the case of the zeroth radial mode for which the waves' radial phase change within the layer where the electrons gyrate is small. In this case, just the plasma parameters cause the main influence on the waves' dispersion properties. In the case of the first and higher radial modes, the wave eigenfrequency is higher and the wavelength is shorter than in the case of the zeroth radial mode. This gain being of interest for practical applications can be achieved without any change in the device design. The possibility of effective excitation of the higher order radial modes of azimuthal surface waves is demonstrated here. Getting shorter wavelengths of the excited waves in the case of higher radial modes is shown to be accompanied by decreasing growth rates of the waves. The results obtained here are of interest for developing new sources of electromagnetic radiation, in nano-physics and in medical physics.
Ecological factors affecting the accumulation and speciation of arsenic in twelve Australian coastal bivalve molluscs
Authors: Maher William , Waring Joel , Krikowa Frank , Duncan Elliott , Foster Simon
Published: May 2018

Environmental contextKnowledge of the pathways by which arsenic is accumulated and transferred in marine ecosystems is scarce. Molluscs are important keystone organisms providing a link between primary producers (micro and macroalgae) and higher trophic levels such as fish. The present study examines the accumulation and species of arsenic in common bivalve molluscs from south-east Australia to understand the cycling of arsenic in marine food webs. AbstractThe present paper reports the whole-tissue total arsenic concentrations and water-soluble arsenic species in 12 common coastal Australian bivalve mollusc species. Mean arsenic concentrations ranged from 18 to 57 µg g−1 dry mass. Planktivores had significantly less arsenic (20–40 µg g−1; 22 ± 3 µg g−1) than did suspension and deposit feeders (36–57 µg g−1; 43 ± 7 µg g−1), with those associated with fine clay–silt sediments (49 ± 7 µg g−1) having significantly more arsenic than those associated with sand substrates (31 ± 11 µg g−1 ). Most planktivores and suspension feeders had similar arsenic species, with high proportions of arsenobetaine (AB) (64–92 %) and relatively low proportions of other arsenic species (0.55–15.8 %). Lower proportions of AB (13–57 %) and larger proportions of inorganic arsenic (6–7 %) were found in deposit feeders, reflecting increased exposure to inorganic arsenic in sediments. The study indicated that at lower trophic levels, organisms feed on algae and suspended matter containing a range of arsenic species including arsenosugars and AB. The implications for arsenic cycling are that as all bivalve molluscs accumulate AB and are a source of AB in benthic food webs. Because all bivalve molluscs also contained appreciable concentrations of arsenoriboses, precursors are present for the de novo synthesis of AB. As well, deposit feeders have higher proportions of inorganic arsenic that can be metabolised to different end products when ingested by higher trophic organisms
Leaf acclimation strategies to contrasting light conditions in saplings of different shade tolerance in a tropical cloud forest
Authors: Quevedo-Rojas Ana , García-Núñez Carlos , Jerez-Rico Mauricio , Jaimez Ramón , Schwarzkopf Teresa
Published: May 2018

To study the acclimation responses of the leaves of saplings of six tree species when changed to low or high levels of irradiance, we carried out a light exposure experiment. Species representative of contrasting shade tolerance groups were identified across a light gradient in the understorey of a Venezuelan Andean cloud forest. Measured traits included gas exchange, chlorophyll fluorescence, and morphoanatomical, biochemical and optical properties. Saplings were grown for 6 months in a shade-house receiving 20% photosynthetic photon flux (PPF) of full sunlight. Plant samples were then moved to shade-houses receiving low PPF (4%) or high PPF (65%). A factorial model (species × PPF), with repeated measurements (0, 15 and 120 days) was designed. Our results showed that morphological and anatomical traits were more plastic to PPF changes than photosynthetic traits. All species were susceptible to photoinhibition (15 days): shade-intolerant species showed dynamic photoinhibition (120 days), whereas shade-tolerant species presented chronic photoinhibition and the consequent inability to increase C assimilation rates under high PPF. The partially shade-tolerant species showed mixed responses; nonetheless, they exhibited larger adjustments in morphoanatomical and optical properties. Thus the acclimation responses of these species when subject to contrasting light conditions could help to explain their distribution along the light gradient in the understorey.
Exogenous fragmented DNA acts as a damage-associated molecular pattern (DAMP) inducing changes in CpG DNA methylation and defence-related responses in Lactuca sativa
Authors: Vega-Muñoz I. , Feregrino-Pérez Ana A. , Torres-Pacheco I. , Guevara-González R. G.
Published: May 2018

Damage-associated molecular patterns (DAMPs) have been studied recently to understand plant self–nonself recognition in response to attack by biotic and abiotic stresses. Extracellular DNA has emerged as a possible DAMP. As a DAMP DNA seems to function depending on the phylogenetic scale and has been tested in a few plant species. This study aimed to evaluate the possible role of self DNA (sDNA) as a DAMP by analysing changes in CpG DNA methylation and defence-related responses in lettuce (Lactuca sativa L.) as a model plant. sDNA and nonself DNA (nsDNA) from Capsicum chinense Murray (both species belong to the same clade, Asterids) stimulated aberrant seed germination and root growth in lettuce seedlings. Similar resultswere obtained with nsDNA obtained from Acaciella angustissima (Mill.) Britton & Rose plants (belonging to the clade Rosids I), although at significantly higher concentrations. Moreover, in most cases, this behaviour was correlated with hypomethylation of CpG DNA as well as defence responses measured as altered gene expression associated with oxidative burst and production of secondary metabolites (phenylpropanoids) related to coping with stress conditions. Our results suggested that extracellular and fragmented DNA has a role as a DAMP depending on phylogenetic closeness in plants as lettuce, inducing epigenetic, genetic and biochemical changes within the plant. The importance of our results is that, for the first time, they demonstrate that sDNA acts as a DAMP in plants, changing CpG DNA methylation levels as well as increasing the production of secondary metabolites associated with defence responses to stress.
Formative research to promote the Get Healthy Information and Coaching Service (GHS) in the Australian-Chinese community
Authors: Cranney Leonie , Wen Li Ming , Xu Huilan , Tam Nancy , Whelan Anna , Hua Myna , Ahmed Nageen
Published: May 2018

The free, telephone-based Get Healthy Information and Coaching Service (GHS) has made sustained improvements in healthy behaviours and weight change in the Australian population, but there is poor uptake of the GHS by culturally and linguistically diverse communities. This formative research study explored the Australian-Chinese community’s awareness, perceptions and experiences of the GHS and their knowledge and cultural beliefs about healthy lifestyles. Conducted in Sydney, Australia, the research included 16 Chinese community-stakeholder interviews, a cross-sectional survey of 253 Chinese community members; and a review of Chinese participant GHS data. The study revealed poor uptake (<1%) and awareness (16%) of the GHS, but good intent (86%) to use it. The need for culturally appropriate and relevant information on healthy eating and physical activity was identified. Employment of a bilingual, bicultural coach, redesign and translation of written resources and targeted promotion in partnership with community organisations were recommended.
Change in distribution of cytoskeleton-associated proteins, lasp-1 and palladin, during uterine receptivity in the rat endometrium
Authors: Nicholson Leigh , Lindsay Laura , Murphy Christopher R.
Published: May 2018

The epithelium of the uterine lumen is the first point of contact with the blastocyst before implantation. To facilitate pregnancy, these uterine epithelial cells (UECs) undergo morphological changes specific to the receptive uterus. These changes include basal, lateral and apical alterations in the plasma membrane of UECs. This study looked at the cytoskeletal and focal adhesion-associated proteins, lasp-1 and palladin, in the uterus during early pregnancy in the rat. Two palladin isoforms, 140 kDa and 90 kDa, were analysed, with the migration-associated 140-kDa isoform increasing significantly at the time of implantation when compared with the time of fertilisation. Lasp-1 was similarly increased at this time, whilst also being located predominantly apically and laterally in the UECs, suggesting a role in the initial contact between the UECs and the blastocyst. This is the first study to investigate palladin and lasp-1 in the uterine luminal epithelium and suggests an importance for these cytoskeletal proteins in the morphological changes the UECs undergo for pregnancy to occur.
Supplementation of cauda epididymal plasma improves sperm characteristics following liquid preservation of ram semen at 3–5°C
Authors: Paul R. K. , Balaganur K. , Bahire S. V. , Kumar D. , Singh R.
Published: May 2018

Mammalian spermatozoa remain immotile and metabolically inactive in the cauda epididymidis, thus maintaining fertility for several weeks. The aim of this study was to functionally characterise and evaluate the effect of cauda epididymal plasma (CEP) on liquid preservation of ram spermatozoa. Four experiments were conducted to investigate the effects of: (1) CEP and its fractions on sperm motility; (2) CEP (10%, 15%, 20% v/v) on liquid preservation of ram spermatozoa; (3) seminal plasma (SP; 20%, 30%, 50% v/v) on liquid-preserved spermatozoa; and (4) both CEP and post-storage SP treatment on sperm characteristics. Biochemical characterisation of ram CEP revealed high protein (30.9 mg mL−1), catalase (68.9 IU mL−1), alkaline phosphatase (17.5 IU mL−1) activities and total antioxidant capacity (1112 µM Trolox equivalent). Progressive motility of prewashed cauda spermatozoa was reduced (P < 0.05) by CEP or its protein-rich fraction compared with protein-free plasma or phosphate-buffered saline. After 48 h storage, total motility, rapid motility (average path velocity >75 µm s−1; 53.9%, 73.5% and 71.8% with 0, 15% and 20% CEP respectively) and straight line velocity (86.3, 102.1 and 102.4 µm s−1 with 0, 15% and 20% CEP respectively) were significantly (P < 0.05) higher in the CEP-treated groups than the control. Viability and acrosomal integrity were similar between groups; however, functional membrane integrity was higher (P < 0.05) in the 15% CEP-treated group. Treatment of liquid-preserved spermatozoa with either 20%, 30% or 50% SP improved (P < 0.05) rapid motility and kinematics at each time point of storage compared with control. In conclusion, liquid preservation of ram spermatozoa in the presence of 15% or 20% CEP and post-storage treatment with SP significantly improve sperm characteristics.
Requests and counters in Russian traffic police officer-citizen encounters
Authors: Márquez Reiter Rosina , Ganchenko Kristina , Charalambidou Anna
Published: May 2018

This paper analyses video recorded interactions between police officers and drivers in traffic stops in Russia. The interactions were recorded via cameras installed on the drivers’ car dashboards, and subsequently uploaded to YouTube; a practice to which over one million Russian motorists have resorted to counterbalance perceived high levels of bribery and corruption (Griaznova 2007). The analysis focuses on responses to opening requests for identification in five different encounters. These show that the drivers repeatedly engage in potentially interpersonally sensitive activities in which the vulnerability of face, especially that of the police officer, is interactionally manifested by launching counter requests in return. The organisation of the request–counter request sequences highlights how face and identity related concerns are interwoven in the participants’ attempts to contest each other’s authority.
Apologies made at the Leveson Inquiry
Authors: Murphy James
Published: May 2018

This paper discusses apologies made by politicians at a recent UK public inquiry, the Leveson Inquiry into the Culture, Practices and Ethics of the Press. I use the freely available data from the Inquiry to explore how politicians apologise in this interactional setting, contrasting it with more usual monologic political apologies. Firstly, I identify the sorts of actions which may be seen as apologisable. I then take a conversation analytic (CA) approach to explore how the apologies can come as a result of an overt complaint and how the apologies are reacted to by counsel and the Inquiry chair. I show that, unlike in everyday conversation, apologies are not the first pair parts of adjacency pairs (cf. Robinson 2004), but rather form action chains (Pomerantz 1978) where the absence of a response is unmarked. I conclude with some observations on how apology tokens may be losing their apologetic meaning.
French-English bilingual children’s motion event communication shows crosslinguistic influence in speech but not gesture
Authors: Miller Nadia , Furman Reyhan , Nicoladis Elena
Published: May 2018

Bilinguals sometimes show crosslinguistic influence from one language to another while speaking (or gesturing). Adult bilinguals have also shown crosslinguistic influence in gestures as well as speech, suggesting an underlying conceptualization that is similar for both languages. The primary purpose of the present study is to test if the same is true of simultaneous French-English bilingual children in speaking and gesturing about motion. If so, they might show different patterns from both French and English monolinguals. Furthermore, we examined whether there were developmental changes between early and middle childhood. French-English bilingual and French and English monolingual children watched two cartoons and described them. In speech, the bilinguals differed from the English monolinguals, using more lexicalizations of the Path of motion in token numbers but not in type. They did not differ from the French monolinguals. In gestures, all children used a majority of Path gestures. There were few age-related changes. We argue that in speech, the bilinguals conceptualize their two languages differently, but show some crosslinguistic influence due to processing. Gestures may not show this same pattern, because they serve to highlight the important parts of the discourse.
A corpus-based contrastive study of impersonal passives in Swedish and Dutch
Authors: Johansson Annika , Rawoens Gudrun
Published: May 2018

This paper deals with impersonal passives in two Germanic languages, Swedish and Dutch. Impersonal passives constitute one type of
impersonal construction (denoting constructions with non-canonical subjects) as described in Siewierska (2008a: 116). Formally, they consist of an overt expletive subject, such as det ‘it’ in
Swedish and er ‘there’ in Dutch, combined with a passive predicate. Semantically, such passive constructions
encode actions with a general reference, i.e. where no agent is specified (cf. Siewierska
1984, Engdahl 2006, Viberg 2010).
The study is corpus-based and uses a bidirectional translation corpus of Swedish and Dutch to map out the specific morphosyntactic
and semantic profile of the impersonal passive in both Swedish and in Dutch. The similarities and differences make these languages
suitable to study from a contrastive perspective in that interesting aspects on impersonal passives are highlighted in the
translation data.
Tag Questions in Conversation
Authors: Kimps Ditte
Published: May 2018

This monograph deals with variable tag questions. These are utterances with a variable interrogative tag, like It's peculiar writing, isn't it, and the semi-variable tag innit, such as Nice, innit. The aim is to provide a corpus-based, comprehensive semantic-pragmatic typology of British English tag questions. Compared to existing descriptions, the proposed typology is novel in three ways. Firstly, whereas almost all existing typologies are single-layered classifications, the functions of tag questions are categorized into two parallel dimensions of interpersonal meaning: the speech function and the stance layer. Secondly, semantic generalizations are proposed for clusters of grammatical, intonational and conversational properties. Thirdly, the bottom-up description is based on a sizeable amount of authentic, spontaneous conversations, which are analysed both qualitatively and quantitatively.
Decomposition of deformed α′(α″) martensitic phase in Ti–6Al–4V alloy
Authors: Motyka Maciej , Baran-Sadleja Anna , Sieniawski Jan , Wierzbinska Malgorzata , Gancarczyk Kamil
Published: May 2018

The process of martensitic α′( α″) phase decomposition in titanium alloys has not been sufficiently characterised in the literature – especially in terms of plastically deformed martensite. The research results of water-quenched Ti–6Al–4V alloy, subsequently cold deformed in compression test and tempered at the temperature range of 600–900°C for 1 and 2 h were presented in the paper. Light and scanning electron microscopy observations revealed the influence of plastic deformation on tempered martensite laths morphology – particularly at the temperature of 900°C – it favoured their fragmentation and spheroidisation. The effect of plastic deformation on characteristic temperatures of α′( α″)→ α +  β phase transformation, phase composition and alloying elements distribution in phase constituents of Ti–6Al–4V alloy was identified and evaluated too.

This paper is part of a thematic issue on Titanium.

GRAPHICAL ABSTRACT
Manifold-like matchbox manifolds
Authors: Clark Alex , Hurder Steven , Lukina Olga
Published: May 2018

A matchbox manifold is a generalized lamination, which is a continuum whose arc-components define the leaves of a foliation of the space. The main result of this paper implies that a matchbox manifold which is manifold-like must be homeomorphic to a weak solenoid.
Komlós’ theorem and the fixed point property for affine mappings
Authors: Domínguez Benavides Tomás , Japón Maria
Published: May 2018

Assume that

X
X

is a Banach space of measurable functions for which Komlós’ Theorem holds. We associate to any closed convex bounded subset

C
C

of

X
X

a coefficient

t
(
C
)

t(C)

which attains its minimum value when

C
C

is closed for the topology of convergence in measure and we prove some fixed point results for affine Lipschitzian mappings, depending on the value of

t
(
C
)
∈
[
1
,
2
]

t(C)\in [1,2]

and the value of the Lipschitz constants of the iterates. As a first consequence, for every

L
>
2

L>2

, we deduce the existence of fixed points for affine uniformly

L
L

-Lipschitzian mappings defined on the closed unit ball of

L
1

[
0
,
1
]

L_1[0,1]

. Our main theorem also provides a wide collection of convex closed bounded sets in

L
1

(
[
0
,
1
]
)

L^1([0,1])

and in some other spaces of functions which satisfy the fixed point property for affine nonexpansive mappings. Furthermore, this property is still preserved by equivalent renormings when the Banach-Mazur distance is small enough. In particular, we prove that the failure of the fixed point property for affine nonexpansive mappings in

L
1

(
μ
)

L_1(\mu )

can only occur in the extremal case

t
(
C
)
=
2

t(C)=2

. Examples are given proving that our fixed point theorem is optimal in terms of the Lipschitz constants and the coefficient

t
(
C
)

t(C)

.
Transference for Banach space representations of nilpotent Lie groups. Part 1. Irreducible representations
Authors: Beltiţă Ingrid , Beltiţă Daniel , Galé José
Published: May 2018

We establish a general CCR (liminarity) property for uniformly bounded irreducible representations of nilpotent Lie groups on reflexive Banach spaces, extending the well-known property of unitary irreducible representations of these groups on Hilbert spaces. We also prove that this conclusion fails for many representations on non-reflexive Banach spaces. Our approach to these results blends the method of transference from abstract harmonic analysis and a systematic use of spaces of smooth vectors with respect to Lie group representations.
The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia
Authors: Shearer Robert F. , Frikstad Kari-Anne Myrum , McKenna Jessie , McCloy Rachael A. , Deng Niantao , Burgess Andrew , Stokke Trond , Patzke Sebastian , Saunders Darren N.
Published: May 2018

Primary cilia are crucial for signal transduction in a variety of pathways, including hedgehog and Wnt. Disruption of primary cilia formation (ciliogenesis) is linked to numerous developmental disorders (known as ciliopathies) and diseases, including cancer. The ubiquitin–proteasome system (UPS) component UBR5 was previously identified as a putative positive regulator of ciliogenesis in a functional genomics screen. UBR5 is an E3 ubiquitin ligase that is frequently deregulated in tumors, but its biological role in cancer is largely uncharacterized, partly due to a lack of understanding of interacting proteins and pathways. We validated the effect of UBR5 depletion on primary cilia formation using a robust model of ciliogenesis, and identified CSPP1, a centrosomal and ciliary protein required for cilia formation, as a UBR5-interacting protein. We show that UBR5 ubiquitylates CSPP1, and that UBR5 is required for cytoplasmic organization of CSPP1-comprising centriolar satellites in centrosomal periphery, suggesting that UBR5-mediated ubiquitylation of CSPP1 or associated centriolar satellite constituents is one underlying requirement for cilia expression. Hence, we have established a key role for UBR5 in ciliogenesis that may have important implications in understanding cancer pathophysiology.
Natural chromatin is heterogeneous and self-associates in vitro
Authors: Cai Shujun , Song Yajiao , Chen Chen , Shi Jian , Gan Lu
Published: May 2018

The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms in which 30-nm fibers have not been observed in vivo: picoplankton and yeast. In the presence of divalent cations, most of the chromatin from both organisms is condensed into a large mass in vitro. Rare irregular 30-nm fibers, some of which include face-to-face nucleosome interactions, do form at the periphery of this mass. In the absence of divalent cations, picoplankton chromatin decondenses into open zigzags. By contrast, yeast chromatin mostly remains condensed, with very few open motifs. Yeast chromatin packing is largely unchanged in the absence of linker histone and mildly decondensed when histones are more acetylated. Natural chromatin is therefore generally nonpermissive of regular motifs, even at the level of oligonucleosomes.
Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking
Authors: Hamada Yuki , Tsurumi Yuta , Nozaki Shohei , Katoh Yohei , Nakayama Kazuhisa
Published: May 2018

The dynein-2 complex mediates trafficking of ciliary proteins by powering the intraflagellar transport (IFT) machinery containing IFT-A and IFT-B complexes. Although 11 subunits are known to constitute the dynein-2 complex, with several light-chain subunits shared by the dynein-1 complex, the overall architecture of the dynein-2 complex has not been fully clarified. Utilizing the visible immunoprecipitation assay, we demonstrated the interaction modes among the dynein-2 subunits, including previously undefined interactions, such as that between WDR60 and the TCTEX1D2–DYNLT1/DYNLT3 dimer. The dynein-2 complex can be divided into three subcomplexes, namely DYNC2H1–DYNC2LI1, WDR34–DYNLL1/DYNLL2–DYNLRB1/DYNLRB2, and WDR60–TCTEX1D2–DYNLT1/DYNLT3. We established cell lines lacking WDR60 or TCTEX1D2, both of which are dynein-2–specific subunits encoded by ciliopathy-causing genes, and found that both WDR60-knockout (KO) and TCTEX1D2-KO cells show defects in retrograde ciliary protein trafficking, with WDR60-KO cells demonstrating more severe defects probably due to failed assembly of the dynein-2 complex. The exogenous expression of a WDR60 mutant lacking TCTEX1D2 binding partially restored retrograde trafficking to a level comparable to that of TCTEX1D2-KO cells. Thus, our results demonstrated that WDR60 plays a major role and TCTEX1D2 plays an auxiliary role in the dynein-2 complex to mediate retrograde ciliary protein trafficking.
Bone Marrow as a Source of Cells for Paroxysmal Nocturnal Hemoglobinuria Detection
Authors: Dulau-Florea Alina E , Young Neal S , Maric Irina , Calvo Katherine R , Dunbar Cynthia E , Townsley Danielle M , Winkler Thomas , Monreal Mariela , Jiang Chunjie , Jordan Elaine K , Braylan Raul C
Published: May 2018

Objectives

To determine fluorescently labeled aerolysin (FLAER) binding and glycophosphatidylinositol–anchored protein expression in bone marrow (BM) cells of healthy volunteers and patients with paroxysmal nocturnal hemoglobinuria (PNH) detected in peripheral blood (PB); compare PNH clone size in BM and PB; and detect PNH in BM by commonly used antibodies.

Methods

Flow cytometry analysis of FLAER binding to leukocytes and expression of CD55/CD59 in erythrocytes. Analysis of CD16 in neutrophils and CD14 in monocytes in BM.

Results

FLAER binds to all normal BM leukocytes, and binding increases with cell maturation. In PNH, lymphocytic clones are consistently smaller than clones of other BM cells. PNH clones are detectable in mature BM leukocytes with high specificity and sensitivity using common antibodies.

Conclusions

PNH clone sizes measured in mature BM leukocytes and in PB are comparable, making BM suitable for PNH assessment. We further demonstrate that commonly used reagents (not FLAER or CD55/CD59) can reliably identify abnormalities of BM neutrophils and monocytes consistent with PNH cells.
RecPhyloXML: a format for reconciled gene trees
Authors: Duchemin Wandrille , Gence Guillaume , Arigon Chifolleau Anne-Muriel , Arvestad Lars , Bansal Mukul S , Berry Vincent , Boussau Bastien , Chevenet François , Comte Nicolas , Davín Adrián A , Dessimoz Christophe , Dylus David , Hasic Damir , Mallo Diego , Planel Rémi , Posada David , Scornavacca Celine , Szöllősi Gergely , Zhang Louxin , Tannier Éric , Daubin Vincent
Published: May 2018

Motivation

A reconciliation is an annotation of the nodes of a gene tree with evolutionary events—for example, speciation, gene duplication, transfer, loss, etc.—along with a mapping onto a species tree. Many algorithms and software produce or use reconciliations but often using different reconciliation formats, regarding the type of events considered or whether the species tree is dated or not. This complicates the comparison and communication between different programs.

Results

Here, we gather a consortium of software developers in gene tree species tree reconciliation to propose and endorse a format that aims to promote an integrative—albeit flexible—specification of phylogenetic reconciliations. This format, named recPhyloXML, is accompanied by several tools such as a reconciled tree visualizer and conversion utilities.

Availability and implementation

http://phylariane.univ-lyon1.fr/recphyloxml/.
Transform-MinER: transforming molecules in enzyme reactions
Authors: Tyzack Jonathan D , Ribeiro Antonio J M , Borkakoti Neera , Thornton Janet M
Published: May 2018

Motivation

One goal of synthetic biology is to make new enzymes to generate new products, but identifying the starting enzymes for further investigation is often elusive and relies on expert knowledge, intensive literature searching and trial and error.

Results

We present Transform Molecules in Enzyme Reactions, an online computational tool that transforms query substrate molecules into products using enzyme reactions. The most similar native enzyme reactions for each transformation are found, highlighting those that may be of most interest for enzyme design and directed evolution approaches.

Availability and implementation

https://www.ebi.ac.uk/thornton-srv/transform-miner
An array-based melt curve analysis method for the identification and classification of closely related pathogen strains
Authors: Hassibi Arjang , Ebert Jessica , Bolouki Sara , Anemogiannis Alexander , Mazarei Gelareh , Li Yuan , Johnson Kirsten A , Van Tran , Mantina Pallavi , Gharooni Taraneh , Jirage Kshama , Pei Lei , Sinha Ruma , Manickam Arun , Zia Amin , Naraghi-Arani Pejman , Schoolnik Gary , Kuimelis Robert G
Published: May 2018

PCR-based techniques are widely used to identify disease causing bacterial and viral pathogens, especially in point-of-care or near-patient clinical settings that require rapid results and sample-to-answer workflows. However, such techniques often fail to differentiate between closely related species that have highly variable genomes. Here, a homogenous (closed-tube) pathogen identification and classification method is described that combines PCR amplification, array-based amplicon sequence verification, and real-time detection using an inverse fluorescence fluorescence-resonance energy transfer technique. The amplification is designed to satisfy the inclusivity criteria and create ssDNA amplicons, bearing a nonradiating quencher moiety at the 5ʹ-terminus, for all the related species. The array includes fluorescent-labeled probes which preferentially capture the variants of the amplicons and classify them through solid-phase thermal denaturing (melt curve) analysis. Systematic primer and probe design algorithms and empirical validation methods are presented and successfully applied to the challenging example of identification of, and differentiation between, closely related human rhinovirus and human enterovirus strains.
Effects of Immunonutrition in Advanced Human Immunodeficiency Virus Disease: A Randomized Placebo-controlled Clinical Trial (Promaltia Study)
Authors: Serrano-Villar Sergio , de Lagarde María , Vázquez-Castellanos Jorge , Vallejo Alejandro , Bernadino José I , Madrid Nadia , Matarranz Mariano , Díaz-Santiago Alberto , Gutiérrez Carolina , Cabello Alfonso , Villar-García Judit , Blanco José Ramón , Bisbal Otilia , Sainz Talía , Moya Andrés , Moreno Santiago , Gosalbes María José , Estrada Vicente
Published: May 2018

Background

While nutritional interventions with prebiotics and probiotics seem to exert immunological effects, their clinical implications in human immunodeficiency virus (HIV)–infected subjects initiating antiretroviral therapy (ART) at advanced HIV disease remain unclear.

Methods

This was a pilot multicenter randomized, placebo-controlled, double-blind study in which 78 HIV-infected, ART-naive subjects with <350 CD4 T cells/μL or AIDS were randomized to either daily PMT25341 (a mixture of synbiotics, omega-3/6 fatty acids and amino acids) or placebo for 48 weeks, each in combination with first-line ART. Primary endpoints were changes in CD4 T-cell counts and CD4/CD8 ratio from baseline to week 48 and safety. Secondary endpoints were changes in markers of T-cell activation, bacterial translocation, inflammation, and α and β microbiota diversity.

Results

Fifty-nine participants completed the follow-up with a mean CD4+ T-cell count of 221 ± 108 cells/μL and mean CD4/CD8 ratio of 0.26 ± 0.19. PMT25341 was well tolerated, without grade 3–4 adverse effects attributable to the intervention. While most of the assessed biomarkers improved during the follow-up in both arms, PMT25341-treated subjects did not experience any significant change, compared to placebo-treated subjects, in mean CD4+ T-cell count change (278 vs 250 cells/μL, P = .474) or CD4/CD8 ratio change (0.30 vs 0.32, P = .854). Similarly, we did not detect differences between treatment arms in secondary endpoints.

Conclusions

In HIV-infected patients initiating ART at advanced disease, the clear immunological benefits of ART were not enhanced by this nutritional intervention targeting the gut-associated lymphoid tissue and microbiota.

Clinical Trials Registration

NCT00870363.
Opportunities and challenges in developing deep learning models using electronic health records data: a systematic review
Authors: Xiao Cao , Choi Edward , Sun Jimeng
Published: May 2018

Objective

To conduct a systematic review of deep learning models for electronic health record (EHR) data, and illustrate various deep learning architectures for analyzing different data sources and their target applications. We also highlight ongoing research and identify open challenges in building deep learning models of EHRs.

Design/method

We searched PubMed and Google Scholar for papers on deep learning studies using EHR data published between January 1, 2010, and January 31, 2018. We summarize them according to these axes: types of analytics tasks, types of deep learning model architectures, special challenges arising from health data and tasks and their potential solutions, as well as evaluation strategies.

Results

We surveyed and analyzed multiple aspects of the 98 articles we found and identified the following analytics tasks: disease detection/classification, sequential prediction of clinical events, concept embedding, data augmentation, and EHR data privacy. We then studied how deep architectures were applied to these tasks. We also discussed some special challenges arising from modeling EHR data and reviewed a few popular approaches. Finally, we summarized how performance evaluations were conducted for each task.

Discussion

Despite the early success in using deep learning for health analytics applications, there still exist a number of issues to be addressed. We discuss them in detail including data and label availability, the interpretability and transparency of the model, and ease of deployment.