bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial Resistance: KAP of Healthcare Professionals at a Tertiary-Level Hospital in Nepal
Authors: Cheoun Mee-Lang , Heo Jongho , Kim Woong-Han
Published: September 2021

Although increasing antimicrobial resistance (AMR) is a substantial threat worldwide, low- and middle-income countries, including Nepal, are especially vulnerable. It is also known that healthcare providers (HCPs) are the major determinants of antimicrobial misuse. A cross-sectional, self-administered survey was conducted among 160 HCPs to assess the knowledge, attitudes, and practices (KAP) of Nepali HCPs regarding AMR and its use. Descriptive statistics and nonparametric tests were performed to evaluate KAP dimensions and investigate subgroup differences. HCPs scored higher on theoretical than practical knowledge. Regarding practical knowledge, men scored higher than women (p < 0.01), and physicians scored higher than nurses (p < 0.001). Participants aged < 25 years scored lower on practical knowledge than older participants (p < 0.001), while those with <3 years work experience scored lower than those with >6 years (p < 0.05). Participants from the medical department scored higher on practical knowledge than those from the surgical department (p < 0.01). AMR control was more accepted in the medical than in the surgical department (p < 0.001). Regarding practices, women and nurses scored higher than men (p < 0.001) and physicians (p < 0.01), respectively. An educational intervention that is tailored to the sociodemographic and professional characteristics of HCPs is necessary to reduce the gap between theoretical and practical knowledge and improve their attitudes and practices.
Determinants of Users’ Attitude and Intention to Intelligent Connected Vehicle Infotainment in the 5G-V2X Mobile Ecosystem
Authors: Yu Zhiyuan , Jin Doudou
Published: September 2021

With the accelerating industrialization of 5G-V2X and smart automobiles, the intelligent connected vehicle (ICV) integrated with sophisticated communication, caching, computing, and control techniques enhance the functionality of in-vehicle infotainment (IVI) and also provide more powerful telematic or entertainment choices in vehicular environment. The diverse needs of ICV users (e.g., drivers and passengers) can be satisfied during commuting and traveling. However, considering the limitations of transportation environment, the potential attitude and usage behavior for the upcoming ICV infotainment directly impacts on the traffic and road safety in sustainable cities. In this paper, we conduct an online and offline survey to investigate the key factors influencing the user attitude and intention of ICV infotainment, where the answers of a total of 502 valid respondents (i.e., IVI users) are collected in China. A conceptual technology acceptance model with the constructs of perceived usefulness (PU), perceived ease of use (PEOU), social influence (SI), consumer innovation (CI), and perceived risk (PR) is established, and then assessed via partial least square structural equation modeling. We find that the constructs of PU, PEOU, CI, and SI have a direct impact on attitude and usage intention, of which 46.8% and 73.4% of variance, respectively, are explained. The respondents show positive attitudes and higher usage intention towards the ICV infotainment. Although PR has insignificant path with attitude and intention, the driving experience moderation effect exists between PR and usage intention. We can see that ICV infotainment will become a trend in future transportation scenario. Through this survey, reference for traffic safety and usage norms will be provided to reduce the risky of public health issues (e.g., traffic accidents) in the context of ICV infotainment.
Depression as Compared to Level of Physical Activity and Internet Addiction among Polish Physiotherapy Students during the COVID-19 Pandemic
Authors: Zalewska Anna , Gałczyk Monika , Sobolewski Marek , Białokoz-Kalinowska Irena
Published: September 2021

Objectives: The aim of the survey was to assess the level of depression correlated with physical activity and internet addiction among physiotherapy students of Polish universities during the COVID-19 pandemic. Methods: The survey was carried out via the internet among Polish physiotherapy students (141 respondents). The level of depression was assessed by the Beck Depression Inventory, physical activity by the International Physical Activity Questionnaire (IPAQ) in Polish and the level of internet addiction by the Kimberly Young Questionnaire. Results: It was found that 31% of those surveyed stated that they suffered from moderate or severe depression. The overwhelming majority of the respondents (92%) considered the level of their internet addiction as low. More physical activity had a positive effect on mental health. The overuse of the internet exacerbated depressive symptoms. Conclusions: The prevalence of depression observed in students is mainly related to distant learning systems. Therefore, regular physical activity is recommended as it is associated with a lower level of depression. It is also advisable to provide students with necessary psychological care. Excessive use of social media is not recommended to elevate mood as it makes depression symptoms worse.
A New Interprofessional Community-Service Learning Program, HATS (Health Ambassador Teams for Seniors) to Improve Older Adults Attitudes about Telehealth and Functionality
Authors: Jurivich Donald , Schimke Carter , Snustad Dakota , Floura Mitchell , Morton Casey , Waind Marsha , Holloway Jeremy , Janssen Sclinda , Danks Meridee , Semmens Karen , Manocha Gunjan Dhawan
Published: September 2021

Senior population health often is underrepresented in curricula for medical and allied health students. Furthermore, entrenched and dense curricular schedules preclude interprofessional teams from clinical experiences related to senior population health. Community service learning potentially offers the opportunity to engage interprofessional students with a panel of older adults to assess health promotion metrics over time. To test this educational concept, we created Health Ambassador Teams for Seniors, also known as HATS. Utilizing a telehealth platform, interprofessional student teams were tasked with older adult wellness promotion. The annual Medicare wellness exam served as a template for patient encounters which was enhanced with key elements of geriatric assessment such as gait and balance, cognition, and functional evaluations. The objective was to have dyads of interprofessional students conduct telehealth visits and gather healthcare data to be used for serial patient encounters and track functional trajectories over time. As a proof of concept, pilot telehealth encounters with medical, physical therapy, nursing and occupational therapy students revealed that data on older adult functional performances such as gait speed, Timed Up and Go test (TUG), and Mini-Cog test could be acquired through telehealth. Equally importantly, trainees received diverse feedback from faculty, peers and volunteer patients. A Research Electronic Data Capture (REDCap) data repository allows trainees to track patient trends relative to their health promotion recommendations as well as handoff their patient panel to the next set of trainees. The HATS program promises to strengthen the Geriatric Workforce, especially with senior population health.
Evaluations of Interventions with Child Domestic Workers: A Rapid Systematic Review
Authors: Kyegombe Nambusi , Pocock Nicola S. , Chan Clara W. , Blagbrough Jonathan , Zimmerman Cathy
Published: September 2021

Little is known about interventions to support the education, skills training, and health of female child domestic workers (CDWs). This rapid systematic literature review followed PRISMA guidelines (PROSPERO registration: CRD42019148702) and summarises peer-reviewed and grey literature on health, education, and economic interventions for CDWs and interventions targeting employers. We searched six electronic databases and purposively searched grey literature. We included observational studies, which included an intervention, quasi-experimental, and experimental studies. Two reviewers independently screened articles. Data were extracted on intervention description, inputs, activities, type of evaluation, outcomes, effect size or impact where applicable, limitations, and ethical considerations. All studies were quality appraised. We identified eight papers from five studies. Six papers reported on health-related outcomes, two on education-related outcomes, and three on economic outcomes. No evaluations of employer-related interventions were identified. Only one intervention specifically targeted CDWs. Others included CDWs in their sample but did not disaggregate data for CDWs. Findings suggest that the evaluated interventions had a limited impact on CDW’s health, education, and economic outcomes. While it appears feasible to reach CDWs with outreach interventions, further work is needed to improve the consistency of their effectiveness and their ability to improve CDWs’ current and future prospects.
Assessment of Referees in Terms of Building a Positive Climate and Responsiveness to the Health, Emotional, and Social Needs of Rugby Players in Competitive Children Sport
Authors: Płoszaj Katarzyna , Firek Wiesław , Ciszewska-Hołda Paulina
Published: September 2021

One of the key elements of effective sports programs that contribute to a child’s sense of joy and satisfaction in participating in sports is contact with an adult who builds a caring climate deliberately and intentionally. Referees play a special role in providing positive experiences for young athletes. The main aim of this study was to assess the quality of referee–player interactions in terms of building a positive climate during the game and the referee’s responsiveness to the health, emotional, social, and cognitive needs of young rugby players aged 6–12 years. The research was conducted among 23 referees refereeing children’s rugby matches in Poland. The structure observation method and The Referee–Players’ Interaction Assessment Scoring System tool were used in the study. Each referee was observed twice. The referee’s verbal and non-verbal communication was recorded using a GoPro Hero 8 camera and an Ejeas Fbim wireless intercom system. A Wilcoxon signed-rank test was used to examine differences between referees’ ratings on two dimensions. The Mann-Whitney U test was employed to test the significance of differences in the mean scores of referees between groups divided by experience. The referees, in both dimensions (building positive climate and responsiveness to the players’ needs), scored on a 7-point scale, were rated at an average level of 3.22 (SD = 1.65) and 4.39 (SD = 1.67), respectively, with the responsiveness dimension rating being statistically significantly higher than the positive climate rating. The results showed that referee experience was not a determinant of the rating of the quality of referee–players’ interactions in both dimensions. From these studies, it was concluded that there is a need to complement the existing training programs for referees with the contents from the field of pedagogy and developmental psychology. This will help them build a positive climate during the game and show how to respond to children’s health, cognitive, emotional, and social needs. Furthermore, developing a referee’s pedagogical skills can improve their relationship with players, which can ultimately translate into a greater sense of job satisfaction. The formulated practical implications can also be transposed to other team sports.
Feasibility of Using Bioelectrical Impedance Analysis for Assessing Youth Weight and Health Status: Preliminary Findings
Authors: Howe Cheryl A. , Corrigan Riley J. , Djalali Maya , McManaway Chris , Grbcich Alexandra , Aidoo Grace Sam
Published: September 2021

Background. This study assessed the accuracy of bioimpedance analysis (BIA) for measuring body composition and resting metabolic rate (RMR) in fasted and non-fasted state and the prospect of using phase angle (PA) to indicate cellular health in youth. Methods. BIA body composition, RMR, and hydration measures were compared to dual-energy x-ray absorptiometry (DXA), MedGem metabolic analyzer, and urine specific gravity, respectively, at baseline in a fasted state using one-way ANOVAs. Repeated BIAs at 0, 30, 60, 90, and 120 min post-prandial were compared to baseline using repeated-measures ANOVA. Correlations were used to assess the relationship among PA and health (blood lipids and glucose, resting BP) and fitness (grip strength and a 3 min step test) measures. Results. BIA scans (N = 58; 11.4 ± 2.9 y) measured lower body fat % (BF%) in healthy weight youth (BMI < 85th percentile; 16.4 ± 1.1 vs. 25.1 ± 1.0%) and lower visceral adipose tissue (VAT) in males (44.5 ± 2.9 vs. 34.1 ± 6.0 cm2) than DXA and higher RMR in all youth (1244 ± 41 vs. 1104 ± 39 kcals/day), healthy weight (1231 ± 48 vs. 1049 ± 44 kcals/day), and teens (1541 ± 62 vs. 1234 ± 72 kcals/day) than MedGem. Compared to baseline, immediate post-prandial values were significantly higher for BF% (21.4 ± 1.4 vs. 22.0 ± 1.4%) and VAT (45.4 ± 6.1 vs. 46.2 ± 6.2 cm2). PA was significantly correlated with BF% (r = −0.33; p = 0.01), fat-free mass (r = 0.59; p < 0.001), grip strength (r = 0.56; p < 0.001). Conclusions. While more data are needed to confirm these preliminary findings, the results suggest caution is necessary in using BIA to assess aspects of youth health and weight status, especially in males, healthy weight, and teens. However, these preliminary findings do indicate that phase angle maybe be a valuable, non-invasive tool for identifying youth who are heading towards obesity and/or obesity-related health consequences.
Sand Play for 0–8-Year-Old Children’s Health and Development: A Systematic Review Protocol
Authors: Iivonen Susanna , Kettukangas Titta , Soini Anne , Viholainen Helena
Published: September 2021

Sand play may be a significant determinant of health and development in early childhood, but systematically synthesised evidence is absent in the literature. The main objective of this study was to present a planned methodology to systematically review, and synthesise, the evidence regarding sand play and its associations with 0–8-year-old children’s health and development. The systematic review following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols statement was registered to PROSPERO (CRD42021253852). Literature searches will be conducted using information from eight electronic databases. Studies will be included when participating children were aged 0–8 years, settings provided children with exposure to sand environments and/or materials, and child-level outcomes related to physical, cognitive, and/or social–emotional health and development. The search results will be imported to software; duplicates will be removed; and independent double screening, and study quality assessments using appropriate tools, will be conducted. Synthesis without meta-analysis will be conducted for quantitative studies similar in exposure, outcome, and content analysis to qualitative studies. Our overall confidence in each review finding will be assessed. The findings of this systematic review can inform policy makers and early childhood education teachers about the associations between sand play and children’s health and development, and its impact in practice.
Consolidation, Stages of Change, and Loyalty among Users of Public Sports and Health Services Aged 12–16
Authors: Fernández-Martínez Antonio , Dueñas-Dorado Luis Alberto , Teva-Villén María Rosario , Nuviala Alberto
Published: September 2021

There are two main lines of inquiry in the literature on adherence and/or loyalty to the practice of physical activity and to health services: one focuses on the impact of perceived quality of sports and health services and satisfaction with these services on user loyalty, while the other concludes that users with more self-determined motivation at more advanced stages of physical activity display higher levels of physical activity and greater intentions to continue this activity. The objective of this study is to ascertain the impact of different dimensions of sports service quality on satisfaction and loyalty among users aged 12 to 16 years old and to identify any differences between adolescent users at more and less consolidated stages of physical activity. A total of 1717 minors with a mean age of 13.83 ± 1.32 years who practise organised physical activity at public sports centres in Nuevo León (Mexico), 51.5% of whom were boys, participated in the study. The model of structural equations linking quality, satisfaction, and loyalty displayed adequate indices. The results showed that the staff, specific activity, and user satisfaction are predictors of loyalty. Significant differences were only found between minors at consolidated and non-consolidated stages of physical activity in the relationship between service personnel and loyalty. In conclusion, human resources and their deployment are predictive of loyalty towards sports and health services among adolescents.
Implementation of a Follow-Up Program for Intensive Care Unit Survivors
Authors: Villa Marta , Villa Silvia , Vimercati Simona , Andreossi Mara , Mauri Fabrizia , Ferlicca Daniela , Rona Roberto , Foti Giuseppe , Lucchini Alberto
Published: September 2021

In Intensive Care Unit (ICU) survivors, critical illness has an impact on an individual’s long-term health status and quality of life. Adults who have recovered from intensive care management could develop muscle weakness, neurocognitive impairment, difficulties in managing activities of daily living and to returning to work, and psychological problems such as depression and anxiety. A prospective, observational study was designed. Data were collected from January to December 2018 from a structured follow-up program, at 3 time points after ICU discharge: at seven days, a visit in the general ward, a phone interview at three months and an ambulatory visit at six months. A total of 95 patients were enrolled, 36% female, with a median age of 65 (55–73) years and a median ICU length of stay of 13 (8–20) days. At the seven days follow-up, patients who had a positive Hospital Anxiety and Depression Scale showed a significantly longer time of intubation (p = 0.048) and length of ICU stay (p = 0.023). At three months, we observed a significant relationship between a positive Hospital Anxiety and Depression Scale and a median value of EuroQol-5D (p = 0.048). At six months, we observed that patients who had a positive Post-Traumatic Symptom Scale were significantly younger than the other group. Findings from the present study suggest that a longer time of intubation and length of ICU stay are associated with a higher level of anxiety and depression immediately after ICU discharge. Follow-up programs are recommended to assess and rehabilitate cognitive function at ICU discharge.
Intimate Partner Violence against Women Living in Inadequate Conditions in Sub-Saharan Africa: A Meta-Analysis of Demographic and Health Survey Data
Authors: Gao Yaqing , Wang Yinping , Mi Xiaoyi , Zhou Mo , Zou Siyu , Zhou Hong
Published: September 2021

Intimate partner violence (IPV) against women is a major public health problem and is widespread in sub-Saharan Africa (SSA). However, little is known about its environmental determinants. This study aimed to investigate whether inadequate living conditions are associated with IPV victimization in women in SSA. We analyzed cross-sectional data for 102,714 women in 25 SSA countries obtained from the Demographic and Health Surveys Program. Logistic regression was used to estimate the country-specific effects of inadequate living conditions (housing with at least one of four characteristics of unimproved water, unimproved sanitation, insufficient space, and unfinished materials) on multiple forms of IPV. Random effects meta-analysis was used to combined the country-specific estimates. We found an association between inadequate living conditions and a higher likelihood of experiencing any (OR = 1.12, 95% CI 1.03 to 1.23, p = 0.012), sexual (OR = 1.18, 95% CI 1.05 to 1.34, p = 0.008), emotional (OR = 1.12, 95% CI 1.02 to 1.23, p = 0.023), and physical (OR = 1.15, 95% CI 1.03 to 1.28, p = 0.010) IPV. The associations were stronger for rural and less-educated women. These findings suggest that future research to establish a causal link between living conditions and IPV and to elucidate the underlying pathways is crucial to design IPV interventions in SSA.
A Systematic Review of the Impact of Wildfires on Sleep Disturbances
Authors: Isaac Fadia , Toukhsati Samia R. , Di Benedetto Mirella , Kennedy Gerard A.
Published: September 2021

Wildfires present a serious risk to humans as well as to the environment. Wildfires cause loss of lives, economic losses, expose people to personal as well as collective trauma, and compromise the mental health of survivors. Sleep disturbances are highly prevalent following a traumatic event; however, their prevalence is not well established amongst those confronted by natural disasters such as wildfires. The aim of this systematic review is to synthesise the empirical findings pertaining to wildfires and the prevalence of sleep disturbances in the general community affected by this natural disaster. We searched EBSCO, PsychINFO, Medline, SpringerLink, CINAHL Complete, EMBASE, PubMed, Scopus and Cochrane Library between January 2012 and March 2021. Five studies met the inclusion criteria. Findings from this systematic review suggest that sleep disturbances, assessed one to ten months following the fires, are highly prevalent in wildfire survivors, with insomnia (ranging between 63–72.5%) and nightmares (ranging between 33.3–46.5%), being the most prevalent sleep disturbances reported in this cohort. Results also highlight the significant associations between sleep disturbances and post-traumatic symptoms following the trauma of wildfires. There is a possible link between sleep disturbance prevalence, severity of, and proximity to fires.
Unilateral and Bilateral Post-Activation Performance Enhancement on Jump Performance and Agility
Authors: Escobar Hincapié Alejandro , Agudelo Velásquez Carlos Alberto , Ortiz Uribe Mariluz , García Torres Camilo Andrés , Rojas Jaramillo Andrés
Published: September 2021

This study aimed to compare the effects of the post-activation performance enhancement (PAPE) of two different types of warm-ups, unilateral and bilateral, on the performance in vertical jumping and agility of healthy subjects with strength training experience. In the study, 17 subjects (12 men and 5 women) performed two different PAPE protocols: unilateral squat (UT) and bilateral squat (BT). The height of the subjects’ countermovement jump (CMJ) and the subjects’ time to perform the T-agility test (TAT) were measured before and after executing the PAPE warm-up. The squats were performed at a velocity of 0.59 m·s−1 with three sets of three repetitions, with a 3-min rest between sets and a 5-min rest after both uni- and bilateral PAPE warm-ups before taking the tests again. For statistical analysis, we applied ANOVA and calculated the effect size. The results showed that the PAPE for each case decreased the CMJ height but generated significant improvements in the total time taken for the T-agility test (p < 0.01); however, in both cases, the effect sizes were trivial. In conclusion, it is possible to observe that the PAPE, performed both unilaterally and bilaterally, negatively affects the performance in the vertical jump, showing moderate effect sizes. However, both PAPE protocols show performance benefits in agility tests, with a large effect size for the unilateral protocol and moderate for the bilateral protocol.
The Utilization of Alkali-Activated Lead–Zinc Smelting Slag for Chromite Ore Processing Residue Solidification/Stabilization
Authors: Yu Lin , Fang Lu , Zhang Pengpeng , Zhao Shujie , Jiao Binquan , Li Dongwei
Published: September 2021

Lead–zinc smelting slag (LZSS) is regarded as a hazardous waste containing heavy metals that poses a significant threat to the environment. LZSS is rich in aluminosilicate, which has the potential to prepare alkali-activated materials and solidify hazardous waste, realizing hazardous waste cotreatment. In this study, the experiment included two parts; i.e., the preparation of alkali-activated LZSS (pure smelting slag) and chromite ore processing residue (COPR) solidification/stabilization. Single-factor and orthogonal experiments were carried out that aimed to explore the effects of various parameters (alkali solid content, water glass modulus, liquid–solid ratio, and initial curing temperature) for alkali-activated LZSS. Additionally, compressive strength and leaching toxicity were the indexes used to evaluate the performance of the solidified bodies containing COPR. As a result, the highest compressive strength of alkali-activated LZSS reached 84.49 MPa, and when 40% COPR was added, the strength decreased to 1.42 MPa. However, the leaching concentrations of Zn and Cr from all the solidified bodies were far below the critical limits (US EPA Method 1311 and China GB5085.3-2007). Heavy-metal ions in LZSS and COPR were immobilized successfully by chemical and physical means, which was detected by analyses including environmental scanning electron microscopy with energy-dispersive spectrometry, Fourier transform infrared spectrometry, and X-ray diffraction.
Setting Criterion for Adolescent Circuit Exercise Program
Authors: Han Yeon-Oh , Lee Byung-Sun , Shin Seon-Yeong
Published: September 2021

The purpose of this study was to develop criteria for an adolescent circuit exercise program. The subjects of this study were 5268 middle- and high-school students. It consisted of three types of circuit exercise programs which were conducted in the physical education class. In the result of this study, we have found two significant finding. First, there were statistically significant differences by grade level and gender in three types of circuit exercise programs. Second, in order to improve the utilization rate and convenience of various adolescents’ physical activity environments and the field of school physical education, the gender of each circuit exercise program was classified and the five-grade evaluation criteria were presented. The criteria for circuit exercise program developed in this study will be utilized for various youth physical activities to contribute to improving health and physical fitness. In addition, physical education teachers are expected to use this criteria as a standard for evaluating the physical fitness level of adolescents.
Spatiotemporal Analysis of Land Cover and the Effects on Ecosystem Service Values in Rupandehi, Nepal from 2005 to 2020
Authors: KC Aman , Wagle Nimisha , Acharya Tri Dev
Published: September 2021

Land cover (LC) is a crucial parameter for studying environmental phenomena. Cutting-edge technology such as remote sensing (RS) and cloud computing have made LC change mapping efficient. In this study, the LC of Rupandehi District of Nepal were mapped using Landsat imagery and Random Forest (RF) classifier from 2005 to 2020 using Google Earth Engine (GEE) platform. GEE eases the way in extracting, analyzing, and performing different operations for the earth’s observed data. Land cover classification, Centre of gravity (CoG), and their trajectories for all LC classes: agriculture, built-up, water, forest, and barren area were extracted with five-year intervals, along with their Ecosystem service values (ESV) to understand the load on the ecosystem. We also discussed the aspects and problems of the spatiotemporal analysis of developing regions. It was observed that the built-up areas had been increasing over the years and more centered in between the two major cities. Other agriculture, water, and forest classes had been subjected to fluctuations with barren land in the decreasing trend. This alteration in the area of the LC classes also resulted in varying ESVs for individual land cover and total values for the years. The accuracy for the RF classifier was under substantial agreement for such fragmented LCs. Using LC, CoG, and ESV, the paper discusses the need for spatiotemporal analysis studies in Nepal to overcome the current limitations and later expansion to other regions. Studies such as these help in implementing proper plans and strategies by district administration offices and local governmental bodies to stop the exploitation of resources.
Transcriptomic Analysis Reveals Differential Expression of Genes between Lung Capillary and Post Capillary Venules in Abdominal Sepsis
Authors: Rahman Milladur , Ding Zhiyi , Rönnow Carl-Fredrik , Thorlacius Henrik
Published: September 2021

Lung endothelial cell dysfunction plays a central role in septic-induced lung injury. We hypothesized that endothelial cell subsets, capillary endothelial cells (capEC) and post capillary venules (PCV), might play different roles in regulating important pathophysiology in sepsis. In order to reveal global transcriptomic changes in endothelial cell subsets during sepsis, we induced sepsis in C57BL/6 mice by cecal ligation and puncture (CLP). We confirmed that CLP induced systemic and lung inflammation in our model. Endothelial cells (ECs) from lung capillary and PCV were isolated by cell sorting and transcriptomic changes were analyzed by bioinformatic tools. Our analysis revealed that lung capEC are transcriptionally different than PCV. Comparison of top differentially expressed genes (DEGs) of capEC and PCV revealed that capEC responses are different than PCV during sepsis. It was found that capEC are more enriched with genes related to regulation of coagulation, vascular permeability, wound healing and lipid metabolic processes after sepsis. In contrast, PCV are more enriched with genes related to chemotaxis, cell–cell adhesion by integrins, chemokine biosynthesis, regulation of actin filament process and neutrophil homeostasis after sepsis. In addition, we predicted some transcription factor targets that regulate a significant number of DEGs in sepsis. We proposed that targeting certain DEGs or transcriptional factors would be useful in protecting against sepsis-induced lung damage.
Mitochondrial Genomes, Phylogenetic Associations, and SNP Recovery for the Key Invasive Ponto-Caspian Amphipods in Europe
Authors: Mamos Tomasz , Grabowski Michał , Rewicz Tomasz , Bojko Jamie , Strapagiel Dominik , Burzyński Artur
Published: September 2021

The Ponto-Caspian region is the main donor of invasive amphipods to freshwater ecosystems, with at least 13 species successfully established in European inland waters. Dikerogammarus spp. and Pontogammarus robustoides are among the most successful, due to their strong invasive impact on local biota. However, genomic knowledge about these invaders is scarce, while phylogeography and population genetics have been based on short fragments of mitochondrial markers or nuclear microsatellites. In this study, we provide: (i) a reconstruction of six mitogenomes for four invasive gammarids (D. villosus, D. haemobaphes, D. bispinosus, and P. robustoides); (ii) a comparison between the structure of the newly obtained mitogenomes and those from the literature; (iii) SNP calling rates for individual D. villosus and D. haemobaphes from different invasion sites across Europe; and (iv) the first time-calibrated full mitogenome phylogeny reconstruction of several Ponto-Caspian taxa. We found that, in comparison to other gammarids, the mitogenomes of Ponto-Caspian species show a translocation between the tRNA-E and tRNA-R positions. Phylogenetic reconstruction using the mitogenomes identified that Ponto-Caspian gammarids form a well-supported group that originated in the Miocene. Our study supports paraphyly in the family Gammaridae. These provided mitogenomes will serve as vital genetic resources for the development of new markers for PCR-based identification methods and demographic studies.
Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties
Authors: Belinskaia Daria A. , Voronina Polina A. , Shmurak Vladimir I. , Jenkins Richard O. , Goncharov Nikolay V.
Published: September 2021

Being one of the main proteins in the human body and many animal species, albumin plays a decisive role in the transport of various ions—electrically neutral and charged molecules—and in maintaining the colloidal osmotic pressure of the blood. Albumin is able to bind to almost all known drugs, as well as many nutraceuticals and toxic substances, largely determining their pharmaco- and toxicokinetics. Albumin of humans and respective representatives in cattle and rodents have their own structural features that determine species differences in functional properties. However, albumin is not only passive, but also an active participant of pharmacokinetic and toxicokinetic processes, possessing a number of enzymatic activities. Numerous experiments have shown esterase or pseudoesterase activity of albumin towards a number of endogeneous and exogeneous esters. Due to the free thiol group of Cys34, albumin can serve as a trap for reactive oxygen and nitrogen species, thus participating in redox processes. Glycated albumin makes a significant contribution to the pathogenesis of diabetes and other diseases. The interaction of albumin with blood cells, blood vessels and tissue cells outside the vascular bed is of great importance. Interactions with endothelial glycocalyx and vascular endothelial cells largely determine the integrative role of albumin. This review considers the esterase, antioxidant, transporting and signaling properties of albumin, as well as its structural and functional modifications and their significance in the pathogenesis of certain diseases.
Synergistic Inhibition Effect of Chitosan and L-Cysteine for the Protection of Copper-Based Alloys against Atmospheric Chloride-Induced Indoor Corrosion
Authors: Messina Elena , Giuliani Chiara , Pascucci Marianna , Riccucci Cristina , Staccioli Maria Paola , Albini Monica , Di Carlo Gabriella
Published: September 2021

The protection of metals from atmospheric corrosion is a task of primary importance for many applications and many different products have been used, sometimes being toxic and harmful for health and the environment. In order to overcome drawbacks due to toxicity of the corrosion inhibitors and harmful organic solvents and to ensure long-lasting protection, new organic compounds have been proposed and their corrosion inhibition properties have been investigated. In this work, we describe the use of a new environment-friendly anticorrosive coating that takes advantage of the synergism between an eco-friendly bio-polymer matrix and an amino acid. The corrosion inhibition of a largely used Copper-based (Cu-based) alloy against the chloride-induced indoor atmospheric attack was studied using chitosan (CH) as a biopolymer and l-Cysteine (Cy) as an amino acid. To evaluate the protective efficacy of the coatings, tailored accelerated corrosion tests were carried out on bare and coated Cu-based alloys, further, the nature of the protective film formed on the Cu-based alloy surface was analyzed by Fourier-transformed infrared spectroscopy (FTIR) while the surface modifications due to the corrosion treatments were investigated by optical microscopy (OM). The evaluation tests reveal that the Chitosan/l-Cysteine (CH/Cy) coatings exhibit good anti-corrosion properties against chloride attack whose efficiency increases with a minimum amount of Cy of 0.25 mg/mL.
Looking for In Vitro Models for Retinal Diseases
Authors: Alfonsetti Margherita , Castelli Vanessa , d’Angelo Michele , Benedetti Elisabetta , Allegretti Marcello , Barboni Barbara , Cimini Annamaria
Published: September 2021

Retina is a layered structure of the eye, composed of different cellular components working together to produce a complex visual output. Because of its important role in visual function, retinal pathologies commonly represent the main causes of visual injury and blindness in the industrialized world. It is important to develop in vitro models of retinal diseases to use them in first screenings before translating in in vivo experiments and clinics. For this reason, it is important to develop bidimensional (2D) models that are more suitable for drug screening and toxicological studies and tridimensional (3D) models, which can replicate physiological conditions, for investigating pathological mechanisms leading to visual loss. This review provides an overview of the most common retinal diseases, relating to in vivo models, with a specific focus on alternative 2D and 3D in vitro models that can replicate the different cellular and matrix components of retinal layers, as well as injury insults that induce retinal disease and loss of the visual function.
Compensatory Protection of Thioredoxin-Deficient Cells from Etoposide-Induced Cell Death by Selenoprotein W via Interaction with 14-3-3
Authors: Kang Hyunwoo , Jeon Yeong Ha , Ham Minju , Ko Kwanyoung , Kim Ick Young
Published: September 2021

Selenoprotein W (SELENOW) is a 9.6 kDa protein containing selenocysteine (Sec, U) in a conserved Cys-X-X-Sec (CXXU) motif. Previously, we reported that SELENOW regulates various cellular processes by interacting with 14-3-3β at the U of the CXXU motif. Thioredoxin (Trx) is a small protein that plays a key role in the cellular redox regulatory system. The CXXC motif of Trx is critical for redox regulation. Recently, an interaction between Trx1 and 14-3-3 has been predicted. However, the binding mechanism and its biological effects remain unknown. In this study, we found that Trx1 interacted with 14-3-3β at the Cys32 residue in the CXXC motif, and SELENOW and Trx1 were bound at Cys191 residue of 14-3-3β. In vitro binding assays showed that SELENOW and Trx1 competed for interaction with 14-3-3β. Compared to control cells, Trx1-deficient cells and SELENOW-deficient cells showed increased levels of both the subG1 population and poly (ADP-ribose) polymerase (PARP) cleavage by etoposide treatment. Moreover, Akt phosphorylation of Ser473 was reduced in Trx1-deficient cells and was recovered by overexpression of SELENOW. These results indicate that SELENOW can protect Trx1-deficient cells from etoposide-induced cell death through its interaction with 14-3-3β.
Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein
Authors: He Jian , Chen Nan-Nan , Li Zhi-Min , Wang Yuan-Yuan , Weng Shao-Ping , Guo Chang-Jun , He Jian-Guo
Published: September 2021

Exosomes are associated with cancer progression, pregnancy, cardiovascular diseases, central nervous system-related diseases, immune responses and viral pathogenicity. However, study on the role of exosomes in the immune response of teleost fish, especially antiviral immunity, is limited. Herein, serum-derived exosomes from mandarin fish were used to investigate the antiviral effect on the exosomes of teleost fish. Exosomes isolated from mandarin fish serum by ultra-centrifugation were internalized by mandarin fish fry cells and were able to inhibit Infectious spleen and kidney necrosis virus (ISKNV) infection. To further investigate the underlying mechanisms of exosomes in inhibiting ISKNV infection, the protein composition of serum-derived exosomes was analyzed by mass spectrometry. It was found that myxovirus resistance 1 (Mx1) was incorporated by exosomes. Furthermore, the mandarin fish Mx1 protein was proven to be transferred into the recipient cells though exosomes. Our results showed that the serum-derived exosomes from mandarin fish could inhibit ISKNV replication, which suggested an underlying mechanism of the exosome antivirus in that it incorporates Mx1 protein and delivery into recipient cells. This study provided evidence for the important antiviral role of exosomes in the immune system of teleost fish.
Characteristics of Retinitis Pigmentosa Associated with ADGRV1 and Comparison with USH2A in Patients from a Multicentric Usher Syndrome Study Treatrush
Authors: Fakin Ana , Bonnet Crystel , Kurtenbach Anne , Mohand-Said Saddek , Zobor Ditta , Stingl Katarina , Testa Francesco , Simonelli Francesca , Sahel José-Alain , Audo Isabelle , Zrenner Eberhart , Hawlina Marko , Petit Christine
Published: September 2021

In contrast to USH2A, variants in ADGRV1 are a minor cause of Usher syndrome type 2, and the associated phenotype is less known. The purpose of the study was to characterize the retinal phenotype of 18 ADGRV1 patients (9 male, 9 female; median age 52 years) and compare it with that of 204 USH2A patients (111 male, 93 female; median age 43 years) in terms of nyctalopia onset, best corrected visual acuity (BCVA), fundus autofluorescence (FAF), and optical coherence tomography (OCT) features. There was no statistical difference in the median age at onset (30 and 18 years; Mann–Whitney U test, p = 0.13); the mean age when 50% of the patients reached legal blindness (≥1.0 log MAR) based on visual acuity (64 years for both groups; log-rank, p = 0.3); the risk of developing advanced retinal degeneration (patch or atrophy) with age (multiple logistic regression, p = 0.8); or the frequency of cystoid macular edema (31% vs. 26%, Fisher’s exact test, p = 0.4). ADGRV1 and USH2A retinopathy were indistinguishable in all major functional and structural characteristics, suggesting that the loss of function of the corresponding proteins produces similar effects in the retina. The results are important for counseling ADGRV1 patients, who represent the minor patient subgroup.
Identification and Comprehensive Analysis of the Nuclear Factor-Y Family Genes Reveal Their Multiple Roles in Response to Nutrient Deficiencies in Brassica napus
Authors: Zheng Xinrui , Zhang Hao , Zhang Limei , Xu Fangsen , Shi Lei , Wang Sheliang , Hong Juan , Ding Guangda
Published: September 2021

Nuclear Factor-Y (NF-Y) transcription factors play vital roles in plant abiotic stress response. Here, the NF-Y family in Brassica napus, which is hyper-sensitive to nitrogen (N) deprivation, was comprehensively identified and systematically characterized. A total of 108 NF-Y family members were identified in B. napus and categorized into three subfamilies (38 NF-YA, 46 NF-YB and 24 NF-YC; part of the Arabidopsis NF-YC homologous genes had been lost during B. napus evolution). In addition, the expansion of the NF-Y family in B. napus was driven by whole-genome duplication and segmental duplication. Differed expression patterns of BnaNF-Ys were observed in response to multiple nutrient starvations. Thirty-four genes were regulated only in one nutrient deficient condition. Moreover, more BnaNF-YA genes were differentially expressed under nutrient limited environments compared to the BnaNF-YB and BnaNF-YC subfamilies. Sixteen hub genes responded diversely to N deprivation in five rapeseed tissues. In summary, our results laid a theoretical foundation for the follow-up functional study of the key NF-Y genes in B. napus in regulating nutrient homeostasis, especially N.
Comparison of the Feasibility, Efficiency, and Safety of Genome Editing Technologies
Authors: González Castro Nicolás González , Bjelic Jan , Malhotra Gunya , Huang Cong , Alsaffar Salman Hasan
Published: September 2021

Recent advances in programmable nucleases including meganucleases (MNs), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) have propelled genome editing from explorative research to clinical and industrial settings. Each technology, however, features distinct modes of action that unevenly impact their applicability across the entire genome and are often tested under significantly different conditions. While CRISPR-Cas is currently leading the field due to its versatility, quick adoption, and high degree of support, it is not without limitations. Currently, no technology can be regarded as ideal or even applicable to every case as the context dictates the best approach for genetic modification within a target organism. In this review, we implement a four-pillar framework (context, feasibility, efficiency, and safety) to assess the main genome editing platforms, as a basis for rational decision-making by an expanding base of users, regulators, and consumers. Beyond carefully considering their specific use case with the assessment framework proposed here, we urge stakeholders interested in genome editing to independently validate the parameters of their chosen platform prior to commitment. Furthermore, safety across all applications, particularly in clinical settings, is a paramount consideration and comprehensive off-target detection strategies should be incorporated within workflows to address this. Often neglected aspects such as immunogenicity and the inadvertent selection of mutants deficient for DNA repair pathways must also be considered.
Lights, Camera, Interaction: Studying Protein–Protein Interactions of the ER Protein Translocase in Living Cells
Authors: Sicking Mark , Jung Martin , Lang Sven
Published: September 2021

Various landmark studies have revealed structures and functions of the Sec61/SecY complex in all domains of live demonstrating the conserved nature of this ancestral protein translocase. While the bacterial homolog of the Sec61 complex resides in the plasma membrane, the eukaryotic counterpart manages the transfer of precursor proteins into or across the membrane of the endoplasmic reticulum (ER). Sec61 complexes are accompanied by a set of dynamically recruited auxiliary proteins assisting the transport of certain precursor polypeptides. TRAP and Sec62/Sec63 are two auxiliary protein complexes in mammalian cells that have been characterized by structural and biochemical methods. Using these ER membrane protein complexes for our proof-of-concept study, we aimed to detect interactions of membrane proteins in living mammalian cells under physiological conditions. Bimolecular luminescence complementation and competition was used to demonstrate multiple protein–protein interactions of different topological layouts. In addition to the interaction of the soluble catalytic and regulatory subunits of the cytosolic protein kinase A, we detected interactions of ER membrane proteins that either belong to the same multimeric protein complex (intra-complex interactions: Sec61α–Sec61β, TRAPα–TRAPβ) or protein complexes in juxtaposition (inter-complex interactions: Sec61α–TRAPα, Sec61α–Sec63, and Sec61β–Sec63). In the process, we established further control elements like synthetic peptide complementation for expression profiling of fusion constructs and protease-mediated reporter degradation demonstrating the cytosolic localization of a reporter complementation. Ease of use and flexibility of the approach presented here will spur further research regarding the dynamics of protein–protein interactions in response to changing cellular conditions in living cells.