bluuebunny/crossref_metadata_2025 huggingface dataset:
Departamento de Cultura Física (1929-1946) y Dirección General de Educación Física (1947) de la Universidad Nacional de La Plata. Continuidades y rupturas en sus discursos legitimantes
Authors: Kopelovich Pablo
Published: May 2020

Comparamos dos instituciones de la Universidad Nacional de La Plata encargadas de la administración del campo de deportes y de la regulación de los cuerpos de los/as estudiantes: el Departamento de Cultura Física (1929-1946) y la Dirección General de Educación Física (1947-1959). Para el primer caso, se toman variadas fuentes, mientras para el segundo nos centramos en un extenso documento inaugural. Se pretende comprender y comparar los discursos legitimantes de ambas propuestas abordando las siguientes categorías: forma de entender el deporte, su manera de llevar a cabo las clases en términos de sexo-género, y sus vínculos con discursos como el médico, el psicológico y el pedagógico. Las mismas son entendidas en relación al contexto local y nacional, en términos políticos, económicos, culturales, y en relación con la Educación Física.
Estudos e práticas docentes em educação popular: narrativas de um cotidiano de complexidades saúde-doença
Authors: Góes Wany Marcele Costa , Oliveira Ivanilde Apoluceno de
Published: May 2020

Neste artigo, apresentamos o contexto socioeducacional e cultural em um ambiente hospitalar expresso por seus atores como práxis de formação docente mediante a complexidade saúde-doença. As ações educativas são desenvolvidas por um Núcleo de Educação Popular, em um hospital de Belém com jovens e adultos, em sua maioria, mulheres vítimas de escalpelamento. O foco é para os impactos da experiência docente em ambiente hospitalar na formação de professores comprometidos com a inclusão social por meio da educação em espaços não convencionais de ensino. Trata-se de dados parciais de uma pesquisa, em que foi utilizada a técnica da história de vida, dinâmicas pedagógicas, por meio de falas, expressões corporais e desenhos, levantamento bibliográfico sobre temáticas relacionadas ao objeto de estudo e a observação participante. Para sistematização e análise dos dados foram utilizadas técnicas da análise de conteúdo. Os dados apontam que as dimensões do cotidiano no ambiente hospitalar atrelada a experiência educativa favorecem a formação docente inclusiva.
Identifikasi Pabrik Gula Sebagai Industrial Heritage Di Jawa
Authors: Nugroho Purwanto Setyo
Published: May 2020

Sugar Factory is a historical marker of the entry of modern industrialization in Java, the existence of a sugar factory is also a witness to the triumph of the sugar industry in Java which was once the second largest sugar supplier in the world. Sugar mills have certain architectural forms and functions, and are recognized as important architectural heritage. This study aims to identify the existence of sugar factories that once existed in Java, and look for aspects related to the importance of sugar factories as industrial heritage with qualitative descriptive methods. Research findings are expected to be useful for conservation preservation activities. The identification results show there are about 231 sugar factories that have ever existed in Java. At present, only 45 factories are still active. Most active sugar mills are located in East Java. Identification that can lead to the determination of the importance of sugar factories as industrial heritage can be seen from the aspects of location, age, function, ownership, physical condition, architectural style and historical events associated with it.
Karakter Bentuk Hunian Suku Rejang Di Daerah Rawan Gempa (Studi Kasus: Desa Gunung Alam, Kabupaten Lebong)
Authors: Prihatiningrum Atik
Published: May 2020

The knowledge of building housing by the Rejang clan in Gunung Alam Village by trial and error using local materials as well as a simple way of reflecting the uniqueness of the form of shelter in the Rejang clan community residing in mountainous rural areas. The focus of this research is to identify the relationship between the form of shelter and the realization of anticipation of occupancy as its role as adaptive shelter in earthquake prone areas. This research is a qualitative descriptive study describing the form of occupancy of the Rejang Clan by exploring the configuration of the building and its structural system to understand the form of occupancy of the Rejang Clan as an adaptive building in earthquake prone areas. The adaptability of the Rejang clan as a shelter in earthquake-prone areas has been implemented through the form of houses on stilts which have a ratio of widths and lengths of buildings 1: 2 with symmetrical and simple spatial arrangements. Ratio of balanced heights in each structure. However, the use of nails has been found in homes and the addition of space that forms a new mass behind the original mass of housing with brick material becomes a risk in the event of an earthquake in the future. Even so, the Rejang Clan occupancy in Gunung Alam Village is still strong to be inhabited by the Rejang Clan and is adaptive in responding to earthquake shocks.
Preferensi Pengunjung Mahasiswa Generasi Z Masa Kini Terhadap Atribut Learning Space di Perpustakaan Akademik
Authors: Akhmadi Akhmadi , Laksitarini Niken , Nabila Ganesha Puspa
Published: May 2020

Most of the current university students are born in Z Generations (1995-2010). Z Generations are unique, especially on their behavior and determining what they like. It included when they want to study around the area of their university. One of the most common study on universities is the academic library. The current academics library are also demanded to be able on adapting and presenting what Z generations want. The ideal academics library can accommodate the learning activities of this generation. This study aims to find the preferences of Z Generations in determining any learning space which come from the library. It also determining the frequency, duration, favorite floor and with whom visitor usually come to library. This preference refers to the theory of learning space attribute. The research method uses quantitative methods by using the survey and questionnaire of 185 students at the ITB, ITS and Unpad. The results showed that Z generations students agreed with the order of preference theory in learning space attribute. This means the academic librarys on university recently should refer to the theory of learning space attribute, so the library can increase the level of the visitors.
Pengaruh Metode Mengajar Dan Motor Educability Terhadap Keterampilan Renang Gaya Kupu-Kupu
Authors: Maulidin  , Wira Panji Nune
Published: May 2020

Tujuan penelitian ini adalah untuk mengetahui perbedaan pengaruh metode mengajar gaya latihan, dan metode mengajar dengan gaya komando terhadap keterampilan renang gaya kupu-kupu. Penelitian experiment dengan rancangan 2 X 2. Sampel sebanyak 60 orang untuk kemudian dilakukan tes motor educability, selanjutnya diurutkan berdasarkan skor tertinggi, dan diambil 27% kelompok atas dan 27%, masing-masing terdiri dari 15 mahasiswa. Teknik analisis data adalah dengan analisis varians (ANAVA) dua jalur dan dilanjutkan dengan uji tukey pada taraf signifikansi (0,05). Hasil penelitian ini adalah: 1) Metode mengajar gaya komando memiliki pengaruh lebih baik dibandingkan dengan metode mengajar gaya latihan tehadap keterampilan renang gaya kupu-kupu, 2) Terdapat interaksi antara gaya mengajar dengan metode latihan dan gaya mengajar komando dan motor educability tehadap keterampilan renang gaya kupu-kupu, 3) Metode mengajar gaya latihan memiliki pengaruh lebih baik dibandingkan dengan metode mengajar gaya komando bagi mahasiswa dengan kemampuan motor educability tinggi, 4) Metode mengajar gaya komando memiliki pengaruh lebih baik dibandingkan dengan metode mengajar gaya latihan bagi mahasiswa dengan kemampuan motor educability rendah.
Comprehensive Analysis of Clinicopathologic Factors Predictive of an Unfavorable Prognosis in Patients With Acinic Cell Carcinoma of the Parotid Gland
Authors: Park Young Min , Yoon Sun Och , Kim Joo Hyun , Kang Min Seok , Kim Da Hee , Koh Yoon Woo , Kim Se-Heon , Lim Jae-Yol , Choi Eun Chang
Published: May 2020

Objectives. In subset of patients, acinic cell carcinoma (AcCC) exhibits aggressive features such as recurrence, distant metastasis, and mortality. This study aimed to investigate clinicopathologic factors influencing patients’ prognosis and to identify adverse features predictive of an unfavorable prognosis.Methods. Between January 2000 and December 2016, 59 patients with AcCC were enrolled in this study.Results. The patients’ 5-year overall survival rate was 93.3%, and their 5-year recurrence-free survival rate was 80.5%. During the study period, recurrence occurred in 10 patients. The mean time to recurrence after surgery was 26 months (range, 5–60 months). During the study period, three patients died from the disease. Univariate analysis showed that sex, surgical extent, extranodal extension, T classification, and TNM stage were significantly associated with disease recurrence. Multivariate analysis showed that, among the clinicopathologic factors included in the analysis, only TNM stage displayed a statistically significant correlation with disease recurrence.Conclusion. Surgical treatment alone yielded good results for AcCC, and additional treatment did not affect the recurrence-free survival rate or the overall survival rate, even when the resection margin was less than 1 mm. Other pathologic factors did not show prognostic significance for disease recurrence or death.
Traumatic Anterior Atlantoaxial Rotatory Subluxation (Type III) Associated with Type III Odontoid Fracture
Authors: Gahlot Nitesh
Published: May 2020

Case:

A 47-year-old man presented with traumatic anterior atlantoaxial subluxation (type III) associated with type III odontoid fracture with no neurological deficit. He was managed surgically with posterior reduction and C1-C2 fixation and fusion. The association of anterior atlantoaxial rotatory subluxation (Fielding type III) with odontoid fracture (Anderson and D'Alonzo type III) is rare with only 7 reported cases in recent literature.

Conclusions:

This report adds important information regarding the management and presentation of such an injury and also describes a serious complication associated with type III odontoid fractures, which was managed conservatively.
An Extraosseous Aneurysmal Bone Cyst in a 59-Year-Old Man
Authors: AlAqeel Motaz , Nooh Anas , Wang Casey K. , Turcotte Robert
Published: May 2020

Case:

A 59-year-old man presented with a history of a painful, rapidly growing mass on the anteromedial aspect of his thigh. On examination, he had a mobile, hard, and nontender mass. Magnetic resonance imaging revealed a well-encapsulated, heterogeneous mass with high-intensity internal septations. Blood-filled spaces were observed within the mass with no involvement of the underlying normal bony structures. The mass was excised, and the diagnosis of an extraosseous aneurysmal bone cyst was confirmed histologically.

Conclusions:

Extraosseous aneurysmal bone cysts are rare benign lesions that can mimic malignant tumors. Careful clinical and pathological assessment should be performed in such cases.
Treatment of an Acromial Stress Fracture After Reverse Total Shoulder Arthroplasty With Teriparatide
Authors: Lipof Jason S , Southgate Richard D. , Tyler Wakenda K. , Bukata Susan V. , Voloshin Ilya
Published: May 2020

Case:

A 78-year-old woman who underwent reverse total shoulder arthroplasty (RTSA) for proximal humerus fracture developed a Type-3 acromial stress fracture, resulting in increased pain and decreased function 9 months post-op. She was managed nonoperatively with adjunctive teriparatide (FORTEO), and after a 4-month course, she had regained excellent motion and achieved union.

Conclusion:

Teriparatide is a viable adjunct in treating patients nonoperatively with acromial stress fractures after RTSA.
Wrist Extensor Tendon “Floating Fat” Sign: A Strong Indicator to Recognize Occult Fractures in the Distal Radius
Authors: Gromis Jonathan T. , Moore Jhanna A. , Van Kouwenberg Emily , Benitez Carlos L.
Published: May 2020

Case:

A 24-year-old woman presented with traumatic left wrist pain. X-rays were negative for fracture. Computed tomography (CT) showed hemorrhagic fatty effusions within the second and third wrist extensor tendon compartments (the “floating fat” sign) and a tiny cortical defect in the Lister's tubercle. Three weeks later, magnetic resonance imaging demonstrated a nondisplaced distal radius fracture with extensive marrow edema.

Conclusion:

The floating fat sign within the extensor tendon compartments is a critical sign of a distal radius fracture, which can be occult on x-ray and CT.
Promoting positive maternal, newborn, and child health behaviors through a group-based health education and microfinance program: a prospective matched cohort study in western Kenya
Authors: Maldonado Lauren Y. , Songok Julia J. , Snelgrove John W. , Ochieng Christian B. , Chelagat Sheilah , Ikemeri Justus E. , Okwanyi Monica A. , Cole Donald C. , Ruhl Laura J. , Christoffersen-Deb Astrid
Published: May 2020

Background: Chamas for Change (Chamas) is a group-based health education and microfinance program for pregnant and postpartum women that aims to address inequities contributing to high rates of maternal and infant mortality in rural western Kenya. In this prospective matched cohort study, we evaluated the association between Chamas participation and facility-based delivery. We additionally explored the effect of participation on promoting other positive maternal, newborn and child health (MNCH) behaviors. Methods: We prospectively compared outcomes between a cohort of Chamas participants and controls matched for age, parity, and prenatal care location. Between October-December 2012, government-sponsored community health volunteers (CHV) recruited pregnant women attending their first antenatal care (ANC) visits at rural health facilities in Busia County to participate in Chamas. Women enrolled in Chamas agreed to attend group-based health education and microfinance sessions for one year; controls received the standard of care. We used descriptive analyses, multivariable logistic regression models, and random effect models to compare outcomes across cohorts 12 months following enrollment, with α set to 0.05. Results: Compared to controls (n=115), a significantly higher proportion of Chamas participants (n=211) delivered in a health facility (84.4% vs. 50.4%, p<0.001), attended at least four ANC visits (64.0% vs. 37.4%, p<0·001), exclusively breastfed to six months (82.0% vs. 47.0%, p<0·001), and received a CHV home visit within 48 hours postpartum (75.8% vs. 38.3%, p<0·001). In multivariable models, Chamas participants were over five times as likely as controls to deliver in a health facility (OR 5.49, 95% CI 3.12-9.64, p<0.001). Though not significant, Chamas participants experienced a lower proportion of stillbirths (0.9% vs. 5.2%), miscarriages (5.2% vs. 7.8%), infant deaths (2.8% vs. 3.4%), and maternal deaths (0.9% vs. 1.7%) compared to controls. Conclusions: Chamas participation was associated with increased odds of facility-based delivery compared to the standard of care in rural western Kenya. Larger proportions of program participants also practiced other positive MNCH behaviors. Our findings demonstrate Chamas’ potential to achieve population-level MNCH benefits; however, a larger study is needed to validate this observed effect. Trial Registration: ClinicalTrials.gov, NCT03188250 (retrospectively registered 31 May 2017)
Relationships between changes in HIV risk perception and condom use in east Zimbabwe 2003-2013: Population-based longitudinal analyses
Authors: Schaefer Robin , Thomas Ranjeeta , Maswera Rufurwokuda , Kadzura Noah , Nyamukapa Constance , Gregson Simon
Published: May 2020

Background: Perceiving a personal risk for HIV infection is considered important for engaging in HIV prevention behaviour and often targeted in HIV prevention interventions. However, there is limited evidence for assumed causal relationships between risk perception and prevention behaviour and the degree to which change in behaviour is attributable to change in risk perception is poorly understood. This study examines longitudinal relationships between changes in HIV risk perception and in condom use and the public health importance of changing risk perception.Methods: Data on sexually active, HIV-negative adults (15-54 years) were taken from four surveys of a general-population open-cohort study in Manicaland, Zimbabwe (2003-2013). Increasing condom use between surveys was modelled in generalised estimating equations dependent on change in risk perception between surveys. Accounting for changes in other socio-demographic and behavioural factors, regression models examined the bi-directional relationship between risk perception and condom use, testing whether increasing risk perception is associated with increasing condom use and whether increasing condom use is associated with decreasing risk perception. Population attributable fractions (PAFs) were estimated. Results: 1988 males and 3715 females participated in ≥2 surveys, contributing 8426 surveys pairs. Increasing risk perception between two surveys was associated with higher odds of increasing condom use (males: adjusted odds ratio [aOR]=1.39, 95% confidence interval [CI]=0.85-2.28, PAF=3.39%; females: aOR=1.41 [1.06-1.88], PAF=6.59%), adjusting for changes in other socio-demographic and behavioural factors. Those who decreased risk perception were also more likely to increase condom use (males: aOR=1.76 [1.12-2.78]; females: aOR=1.23 [0.93-1.62]) compared to those without change in risk perception.Conclusions: Results on associations between changing risk perception and increasing condom use support hypothesised effects of risk perception on condom use and effects of condom use on risk perception (down-adjusting risk perception after adopting condom use). However, low proportions of change in condom use were attributable to changing risk perception, underlining the range of factors influencing HIV prevention behaviour and the need for comprehensive approaches to HIV prevention.
Prevalence of surgical site infection and its associated factors after cesarean section in Ethiopia: systematic review and meta-analysis
Authors: Getaneh Temesgen , Negesse Ayenew , Dessie Getenet
Published: May 2020

Background Surgical site infection (SSI) affects up to one third of patients who have undergone a surgical procedure. It is a significant cause of surgical patient morbidity, mortality and human and financial costs threat. The national prevalence of SSI among mothers who undergo for cesarean section and its risk factors are not well investigated in Ethiopia. Therefore, this systematic and meta-analysis conducted to estimate the pooled prevalence of surgical site infection and its associated factors after cesarean section in Ethiopia.Methods Original articles were searched in PubMed/MEDLINE, EMBASE, CINAHL, Google Scholar, HINARI portal (which includes the SCOPUS, African Index Medicus, and African Journals Online databases), and Cochrane Library. Data were extracted using a standard data extraction checklist that developed according to Joanna Briggs Institute (JBI). The I2 statistic was used to quantify heterogeneity across studies. Funnel plot asymmetry and Egger’s tests were used to check for publication bias. A fixed effect model was used to estimate the pooled prevalence of surgical site infection. Odds Ratio (OR) with 95% Confidence Interval (CI) was also used to determine the association of identified variables with surgical site infection. Statistical analysis was conducted using STATA software.Result From initial 179 identified articles, 11 were eligible for inclusion in the final meta-analysis. The pooled estimate of surgical site infection after cesarean section in Ethiopia was 9.72% (95%CI: 8.38, 11.05). PROM >12hrs (OR=5.02, 95%CI: 2.65, 9.51), duration of labor>24hrs (OR=3.69, 95%CI: 2.41, 5.65), chorioamnionitis (OR=9.11, 95%CI: 5.21, 15.93), anemia (OR=4.75, 95%CI: 2.42, 9.33) and having vertical skin incision (OR=4.17, 95%CI: 2.90, 6.02) were factors significantly associated with surgical site infection after cesarean section.Conclusion The prevalence of surgical site infection after cesarean section in Ethiopia was high. Therefore, Minister of Health with its stake holders should emphasis on community and institution based programs in manner to prevent those factors listed above that have significant effect on surgical site infection.
Effect of transcranial direct current stimulation on sports performance for two profiles of athletes (power and endurance) (COMPETE): a protocol for a randomised controlled trial.
Authors: Grandperrin Yohan , Grosprêtre Sidney , Nicolier Magali , Gimenez Philippe , Vidal Chrystelle , Haffen Emmanuel , Bennabi Djamila
Published: May 2020

Background

Transcranial direct current stimulation (tDCS) is promising for improving motor and cognitive performance. Nevertheless, its mechanisms of action are unclear and need to be better characterised according to the stimulated brain area and the type of exercise performed.

Methods/design

This is a double-blind cross-over study, organised into two parts: the first is to assess the effects of tDCS on explosive performance (jump task) and the second is to assess the effects on endurance performance (cycling time trial task). Participants, who are recreationally active or athletes (cyclists, parkour practitioners), will receive two active tDCS sessions (over the left dorsolateral prefrontal cortex and right motor cortex) and one sham tDCS session (part A) or two daily tDCS sessions (one active sequence and one sham) over five days (part B). Motor and cognitive performance will be compared before and after the tDCS sessions (part A) and before and after the first session, after the last session and at day 12 and day 30 of each tDCS sequence (part B).

Discussion

This study investigates the acute and long-term effects of tDCS on the motor and cognitive performance in healthy subjects. It will try to evaluate if tDCS could be considered as a neuroenhancement technology according to the physical task investigated (endurance versus explosive).
Dexmedetomidine for prevention of postoperative pulmonary complications in patients after oral and maxillofacial surgery with fibular free flap reconstruction:a prospective, double-blind, randomized, placebo-controlled trial
Authors: Liu Yun , Zhu Xi , Zhou Dan , Han Fang , Yang Xu Dong
Published: May 2020

Background: Postoperative pulmonary complications (PPCs) are common and significant problems for oral and maxillofacial surgery patients. Dexmedetomidine (DEX), an α2-adrenoreceptor agonist, has been proven having lung protection effects. However, since now, there has not been final conclusion about whether DEX can reduce the incidence of PPCs. We hypothesize that, in oral and maxillofacial surgery with fibular free flap reconstruction patients, DEX may decrease the incidence of PPCs.Methods: This was a prospective, double-blind, randomized, placebo-controlled, single-centered trial with two parallel arms. A total of 160 patients at intermediate-to-high risk of PPCs undergoing oral and maxillofacial surgery with fibular free flap reconstruction and tracheotomy were enrolled and randomized to receive continuous infusion of either DEX or placebo (normal saline). 0.4 ug/kg of DEX was given over 10mins as an initial dose followed by a maintaining dose of 0.4 ug/kg/h till the second day morning after surgery. At the same time, the normal saline was administered a similar quantity. The primary outcome was the incidence of PPCs according to Clavien-Dindo score within 7 days after surgery. Results: The two groups had similar characteristics at baseline. 18(22.5%) of 80 patients administered DEX, and 32(40.0%) of 80 patient administered placebo experienced PPCs within the first 7 days after surgery (relative risk [RR] 0.563,95% confidence interval [CI] 0.346-0.916; P=0.017). In the first 7 days after surgery, the DEX group had a lower incidence of PPCs and a better postoperative survival probability (Log-rank test, P=0.019), and was less prone to occur PPCs (Cox regression, P=0.025, HR=0.516). When the total dose of DEX was more than 328μg, the patients were unlikely to have PPCs (ROC curve, AUC=0.614, P=0.009).Conclusions: For patients undergoing oral and maxillofacial surgery with fibular free flap reconstruction and tracheotomy who were at intermediate or high risk of developing PPCs, continuous infusion of DEX could decrease the occurrence of PPCs during the first 7 days after surgery and shorten the length of hospital stay after surgery, but did not increase the prevalence of bradycardia or hypotension. Trial registration: Chinese Clinical Trial Registry, www.chictr.org.cn, number: ChiCTR1800016153; Registered on May 15, 2018.
Feasibility and behavioral effects of prolonged static and dynamic standing as compared to sitting in older adults with type 2 diabetes mellitus
Authors: Marusic Uros , Muller Martijn , Alexander Neil , Bohnen Nicolaas
Published: May 2020

Background: Physical inactivity is prevalent in older adults with type 2 diabetes mellitus (T2DM) and may exacerbate their clinical symptoms. The aim of this study was to examine the feasibility of 4-hour regular versus more dynamic standing sessions while performing routine desktop activities as a non-exercise physical activity intervention in older adults with T2DM to increase non-exercise activity.Methods: Twelve older adult patients with T2DM (3 female; age 71 ± 4 years; BMI 34 ± 5 kg/m2) completed three sessions (baseline sitting followed by "static" or "dynamic" desktop standing sessions). Participants stood behind a regular height-adjustable desk in the "static" standing session. An upright dynamic standing desk, which provides cues to make small weight-shifting movements, was used for the "dynamic" standing session. Oxygen consumption, cognitive performance, as well as net standing duration, total movement activity, and musculoskeletal discomfort were assessed during all three sessions.Results: All participants were able to complete all sessions. Oxygen consumption and overall movements progressively increased from sitting to static and dynamic standing, respectively (p<0.001). The duration of breaks during standing (p=0.024) and rate of total musculoskeletal discomfort development (p=0.043) were lower in the dynamic standing compared to static standing sessions. There was no evidence of executive cognitive worsening during either standing session compared to sitting.Conclusions: Prolonged 4-hour standing as a simple non-exercise physical intervention is feasible in older adults with T2DM and may have metabolic (VO2 consumption) benefits. Increasing movement during desktop standing may offer incremental benefits compared to regular standing. Prolonged desktop standing might provide an effective intervention in T2DM older participants to target sedentariness.
What are the sociodemographic and gender determinants of non-fatal self-harm in older adult users and non-users of antidepressants? A national population-based study
Authors: Hedna Khedidja , Fastbom Johan , Skoog Ingmar , Hensing Gunnel , Waern Margda
Published: May 2020

Background Late-life self-harm (SH) is often linked to depression. However, very few studies have explored the role of other factors and their interaction with depression in the occurrence of late-life SH. The objective of this research was to examine sociodemographic and gender factors associated with non-fatal SH, in older adults with and without antidepressant therapy.Methods We used national longitudinal register data from a total cohort of all Swedish residents aged ≥75 years between 2006-2014 (N=1,413,806). Using personal identity numbers, we linked individuals' data from numerous national registers. We identified all those with at least one episode of non-fatal self-harm (regardless of level of intent to die) and matched 50 controls to each case. A nested case–control design was used to investigate sociodemographic factors associated with non-fatal SH in the total cohort and among antidepressant users and non-users. Risk factors were analysed in adjusted conditional logistic regression models for the entire cohort and by gender.Results In all, 2242 individuals had at least one episode of a non-fatal SH (980 men and 1262 women). Being unmarried was a risk factor for non-fatal SH in men but not in women. Among users of antidepressants, higher non-fatal SH risk was observed in those born outside the Nordic countries (IRR: 1.44; 95% CI: 1.11–1.86), whereas in AD non-users increased risk was seen in those from Nordic countries other than Sweden (IRR: 1.58; 95% CI: 1.08–2.29). Antidepressant users with higher education had an increased risk of non-fatal SH (IRR: 1.34; 95% CI: 1.12–1.61), in both men and women. Conclusions Foreign country of birth was associated with increased risk for non-fatal SH in older adults with and without AD therapies. Being married was a protective factor for non-fatal SH in men. The complex association between sociodemographic factors and use of antidepressants in the occurrence of self-harm in older men and women indicates the need for multifaceted tailored preventive strategies including healthcare and social services alike.
SHiNeMaS : A web tool dedicated to seed lots history, phenotyping and cultural practices
Authors: De Oliveira Yannick , Burlot Laura , Dawson Julie C. , Goldringer Isabelle , Madi Darkawi , Rivière Pierre , Steinbach Delphine , Frank Gaëlle Van , Thomas Mathieu
Published: May 2020

Motivation: In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Reseau Semences Paysannes (RSP) on bread wheat participatory breeding (PPB). The aims were: (1) to study on-farm management of crop diversity, (2) to develop population-varieties adapted to organic and low-inputs agriculture, (3) to co-develop tools and methods adapted to on-farm experiments. In this project, researchers and farmers' organizations needed to map the history and life cycle of the population-varieties using network formalism to represent relationships between seed lots. All this information had to be centralized and stored in a database. Results: We describe here SHiNeMaS (Seeds History and Network Management System) a web tool database. SHiNeMaS aims to provide useful interfaces to track seed lot history and related data (phenotyping, environment, cultural practices). Although SHiNeMaS has been developed in the context of a bread wheat participatory breeding program, the database has been designed to manage any kind and even multiple cultivated plant species. SHiNeMaS is available under A ero GPL licence and uses free technologies such as the Python language, Django framework or PostgreSQL database management system (DBMS). Conclusion: We developed SHiNeMaS, a web tool database, dedicated to the management of the history of seed lots and related data like phenotyping, environmental information and cultural practices. SHiNeMaS has been used in production in our laboratory for 5 years and farmers' organizations facilitators manage their own information in the system.
Characterization of Clostridium novyi isolated from a sow in a sudden death case in Korea
Authors: Jeong Chang-Gi , Seo Byoung-Joo , Nazki Salik , Jung Byung Kwon , Khatun Amina , Yang Myeon-Sik , Kim Seung-Chai , Noh Sang-Hyun , Shin Jae-Ho , Kim Bumseok , Kim Won-Il
Published: May 2020

Background: Multifocal spherical nonstaining cavities and gram-positive, rod-shaped, and endospore-forming bacteria were found in the liver of a sow that died suddenly. Clostridium novyi type B was identified and isolated from the sudden death case, and the isolate was characterized by molecular analyses and bioassays in the current study.Results: C. novyi was isolated from the liver of a sow that died suddenly and was confirmed as C. novyi type B by differential PCR. The C. novyi isolate fermented glucose and maltose and demonstrated lecithinase activity, and the cell-free culture supernatant of the C. novyi isolate exhibited cytotoxicity toward Vero cells, demonstrating that the isolate produces toxins. In addition, whole-genome sequencing of the C. novyi isolate was performed, and the complete sequences of the chromosome (2.29 Mbp) and two plasmids (134 and 68 kbp) were identified for the first time. Based on genome annotation, 7 genes were identified as glycosyltransferases, which are known as alpha toxins; 23 genes were found to be related to sporulation; 12 genes were found to be related to germination; and 20 genes were found to be related to chemotaxis.Conclusion: C. novyi type B was isolated from a sow in a sudden death case and confirmed by biochemical and molecular characterization. Various virulence-associated genes were identified for the first time based on whole-genome sequencing.
Network-based identification of biomarkers for colon adenocarcinoma
Authors: Hu Fuyan , Wang Qing , Yang Zhiyuan , Zhang Zeng , Liu Xiaoping
Published: May 2020

Background: As one of the most common cancers with high mortality in the world, we are still facing a huge challenge in the prevention and treatment of colon cancer. With the rapid development of high throughput technologies, new biomarkers identification for colon cancer has been confronted with the new opportunities and challenges.Methods: We firstly constructed functional networks for each sample of colon adenocarcinoma (COAD) by using a sample-specific network (SSN) method which can construct individual-specific networks based on gene expression profiles of a single sample. The functional genes and interactions were identified from the functional networks, respectively.Results: Classification and subtyping were used to test the function of the functional genes and interactions. The results of classification showed that the functional genes could be used as diagnostic biomarkers. The subtypes displayed different mechanisms, which were shown by the functional and pathway enrichment analysis for the representative genes of each subtype. Besides, subtype-specific molecular patterns were also detected, such as subtype-specific clinical and mutation features. Finally, 12 functional genes and 13 functional edges could serve as prognosis biomarkers since they were associated with the survival rate of COAD.Conclusions: In conclusion, the functional genes and interactions in the constructed functional network could be used as new biomarkers for COAD. And the subtypes could be used in realizing the personalized medicine of COAD.
Efficient generation of site-specific point mutations in cell lines by hyper active CBEs (hyCBEs)
Authors: Zhu Biyun , Chen Liang , Zhang Xiaohui , Li Dali
Published: May 2020

Cytidine base editors (CBEs) are powerful genetic tools which catalyze cytidine to thymidine conversion at specific genomic loci. Further improvement of the editing range and efficiency is critical for their broader applications. In this protocol, we demonstrated that fusion of the single-strand DNA binding domain (ssDBD) from Rad51 protein between Cas9n and cytidine deaminases, serial hyper CBEs (hyCBEs) were generated with substantially increased activity and an expanded editing window toward the protospacer adjacent motif (PAM) in cell lines. Moreover, hyeA3A-BE4max specifically generated a C-to-T conversion without inducing bystander mutations in the hemoglobin gamma (HBG) gene promoter to mimic a naturally occurring genetic variant for amelioration of β-hemoglobinopathy, suggesting the therapeutic potential of the improved base editors. This step-by-step protocol is related to the publication “Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-strand DNA binding protein domain” in Nature Cell Biology.
Differential Influences of Social Support on App Use for Diabetes Self-Management – a Mixed Methods Approach
Authors: Brew-Sam Nicola , Chib Arul , Rossmann Constanze
Published: May 2020

Background The impact of social support on diabetes management and health outcomes has been investigated comprehensively, with recent studies examining social support delivered via digital technologies. This paper argues that social support has an impact on the use of diabetes technologies. Specifically, we postulate differences between the impact of healthcare professional versus non-professional (family/friends) support on mobile app use for diabetes self-management. Methods This research employed a triangulation of methods including exploratory semi-structured face-to-face interviews (N= 21, Study 1) and an online survey (N= 65, Study 2) with adult type 1 and type 2 diabetes patients. Thematic analysis (Study 1) was used to explore the relevance of social support (by professionals versus non-professionals) for diabetes app use. Binary logistic regression (Study 2) was applied to compare healthcare decision-making, healthcare-patient communication, and the support by the personal patient network as predictors of diabetes app use, complemented by other predictors from self-management and technology adoption theory. Results The interviews (Study 1) demonstrated that (technology-supported) shared decision-making and supportive communication by healthcare professionals depended on the medical specialty of attending physicians. The personal patient network was perceived as either facilitating or hindering the use of mHealth for self-management. Binary logistic regression (Study 2) showed that the specialty of the physician significantly predicted the use of diabetes apps, with supervision by diabetes specialists increasing the likelihood of app use (as opposed to general practitioners). In addition, specialist care positively related to a higher chance of shared decision-making and better physician-patient communication. The support by the personal patient network predicted diabetes app use in the opposite direction, with less family/friend support increasing the likelihood of app use. Conclusion The results emphasize the relevance of support by healthcare professionals and by the patient network for diabetes app use and disclose differences from the existing literature. In particular, we found that the use of diabetes apps may increase in the absence of social support by family or friends (e.g., compensation for lack of support), and that use of diabetes apps may decrease when such support is high (e.g., no perceived need to use technology). Implications for practice are discussed.
A unique type of fully covered metal stent for the management of post liver transplant biliary anastomotic strictures
Authors: Warner Ben , Harrison Phillip , Farman Muhammad , Devlin John , Reffitt David , El-Sherif Yasser , Khorsandi Shirin E , Prachalias Andreas , Cerisuelo Miriam Cortes , Menon Krish , Jassem Wayel , Sriniva Parthi , Vilca-Melendez Hector , Heneghan Michael , Heaton Nigel , Joshi Deepak
Published: May 2020

Background: We report our experience of treating anastomotic strictures using a novel type of fully covered metal stent (FCSEMS). This stent, known as the Kaffes Stent, is short-length with an antimigration waist and easily removable due to long retrieval wires deployed within the duodenum. Methods: 62 patients underwent ERCP and Kaffes stent insertion for post-transplant anastomotic strictures following confirmation of a stricture and biliary obstruction on MRCP. These patients were retrospectively analysed for immediate and longterm stricture resolution, improvement in symptoms and liver function tests (LFTs), stricture recurrence and complication rates. Results: 96% had immediate stricture resolution and 81% of patients continued to have long-term resolution (mean follow-up period was 548 days). Of the 16 patients with symptoms of biliary obstruction, 13 had resolution of their symptoms. A further 46 patients were asymptomatic, although 18 of these had cholestatic liver function tests. Overall, there was a significant improvement in LFTs. Complication rates were 15% with only one patient requiring biliary reconstruction. Conclusions: The Kaffes stent is effective and safe at resolving post liver transplant biliary anastomotic strictures.
Clinical Significance of MicroRNA-155 Regulated Autophagy and Apoptosis by targeting gene Rictor/Fos in Gastric Cancer Progression
Authors: Chen Gang , Li Yu , Ren Zhijian , Gu Yanmei , Tang Futian , Mao Jie , Zhu Junmin , Wang Ling , Li Yumin
Published: May 2020

BACKGROUND Microrna-155 plays an important role in the pathogenesis, progression and treatment of various cancers. It is abnormally expressed in gastric cancer, but its expression level, mechanism and significance are controversial in different studies. So we make the study to explore the expression level, significance and mechanism of microRNA-155 on gastric cancer. METHODS Target genes of microRNA-155 in TargetScan and mirDB databases were analyzed by Wayne Mapping, Enrichr database, String database, TIMER database. Forty-three pairs of cancer tissues and adjacent tissues were collected to extract total RNA and protein. Expression of MicroRNA-155, Rictor, Fos, Beclin1, LC3, caspase3 and caspase9 were measured by qRT-PCR and western blot. The relationship between gene expression and clinicopathological factors were analyzed. SPSS 23.0 was used for statistical analysis. RESULTS A total of 700 (miRDB) and 556 (TargetScan) target genes were obtained and 280 genes were in the intersection of Wayne Mapping, 49 of them had a target score of 90 or more. GO and KEGG analysis revealed that they were related to autophagy or apoptosis pathway. Rictor and Fos were selected as research objects. Thirty-two cases showed high microRNA-155 expression (group H) and 11 cases showed low expression (group L). Twelve patients had high Rictor expression and 31 patients had low expression; Thirteen cases had roughly normal Fos expression and 30 cases had low or negative expression; Thirty-three cases had high Beclin1/LC3 expression and 10 cases had low expression; Ten cases had high caspase3/caspase9 expression and 33 cases had low expression. According to the results of immunohistochemistry and western blot, Rictor, Fos, caspase3 and caspase9 were low expressed while Beclin1 and LC3 were high expressed in group H. However, all the six genes had no significant difference in group L. CONCLUSIONS The abnormal expression of microRNA-155 may indicate the occurrence of gastric cancer and its expression level is negatively correlated with clinical stage of cancer. The down-regulated expression of Rictor and Fos, enhancement of autophagy and weakening of apoptosis may be related to the over-expression of microRNA-155. MicroRNA-155 may promote the progression of gastric cancer by promoting autophagy and inhibiting apoptosis.
Correlation between White Blood Cell Count at Admission and Mortality in COVID-19 Patients: A Retrospective Study
Authors: Feng Xiaokai , Zhu Bin , Jiang Chunguo , Mi Song , Yang Liya , Zhao Zhigang , Zhang Yong , Zhang Liming
Published: May 2020

Background: Coronavirus disease-19 (COVID-19) has spread rapidly and has become a world health threaten. Its risk factors with death were still unknow. White blood cells (WBC) as a reflection of inflammation had play a vital role in COVID-19, however its level with death were still not know. Methods: In this retrospective, single-center study, all confirmed patients with COVID-19 on admission at West Branch of Union Hospital from Jan 29 to Feb 28, were collected and analyzed. Demographic and clinical data including laboratory examinations were analyzed and compared between recovery and death patients.Results: A total of 163 patients including 33 death cases were included in this study. Significant associations were found between WBC level and death (HR = 1.14, 95%CI: 1.09-1.20, p<0.001). The regression analysis results showed there was a significant association between WBC level and death (HR = 5.72, 95%CI: 2.21-14.82, p < 0.001) when use the second quartile as a cutoff value (> 6.16×10^9/L). The difference was still existing after we adjusting for confounding factors (HR = 6.26, 95%CI: 1.72-22.77, p = 0.005). In addition, Kaplan-meier survival analysis showed that there was a significant decline of the cumulative survival rate (p < 0.001) in those with WBC level ≥ 6.16×10^9/L.Conclusion: WBC at admission is significantly corelated with death in COVID-19 patients. Higher level of WBC should be given more attention in the treatment of COVID-19.
Monocyte-derived Macrophages: the Supplements of Hepatic Macrophage in Echinococcus Multilocularis Infected Mice
Authors: Li Bin , Qi Xinwei , Liu Yumei , Yan Yi , Shan Jiaoyu , Cai Xuanlin , Lv Jie , Zhou Xuan , Luo Li , Ma Xiumin
Published: May 2020

Background Alveolar echinococcosis (AE) is a potentially lethal zoonosis caused by the cestode Echinococcus multilocularis. The aim of this research is to investigate the dynamic changes of monocytes, macrophages and related cytokines in animal models of persistent infection of Echinococcus multilocularis, specifically exploring the possible immune changes in infection of Echinococcus multilocularis.Methods An infection model was established by intraperitoneal injection of a protoscolex suspension. The pathological changes of mice liver were observed by HE staining and the score scale of infection was established. The ratio of Ly6Chi and Ly6Clo Monocytes in peripheral blood of mice was detected by flow cytometry. The distribution and expression of CX3CL1, CX3CR1, iNOS, CD163 and CD11b in mouse liver were detected by immunohistochemistry. The mRNA expression levels of TNF-α and Arg1 in mouse liver were detected by qRT-PCR. The expression levels of INF-γ, IL-17, IL-4 and IL-10 in peripheral blood of mice were detected by ELISA.Results The results of HE staining showed that significant lesions appeared in the later stages of infection in the liver. The results of the infection degree score scale showed that the severity score value increased with the prolongation of the infection time. The results of flow cytometry showed that the proportion of Ly6Chi monocytes in the peripheral blood of the experimental group mice was decreased after a brief rise, Ly6Clo monocytes decreased first and then increased. The results of immunohistochemistry showed that the expression of CX3CL1, CX3CR1, CD11b, CD163 and iNOS in the mice liver of the experimental group was increased. The results of qRT-PCR showed that the expression level of TNF-α and Arg1 mRNA in the liver of the experimental group mice increased. The results of ELISA showed that the expression level of IFN-γ, IL-17, IL-4 and IL-10 increased with the duration of infection.Conclusions Monocytes as a supplement to hepatic macrophage, monocytes and kupffer cells (KCs) both participate in Th1 and Th2 immune responses of the body by differentiating into M1 or M2 at different stages of Echinococcus multilocularis infection.