bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Intramuscularly Administered Oxytetracycline or Enrofloxacin on Vancomycin-Resistant Enterococci, Extended Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae in Pigs
Authors: González-Fandos Elena , Martínez-Laorden Alba , Abad-Fau Ana , Sevilla Eloisa , Bolea Rosa , Serrano María Jesús , Mitjana Olga , Bonastre Cristina , Laborda Alicia , Falceto María Victoria , Pagán Rafael
Published: March 2022

Nowadays, there is a great concern about the prevalence of multidrug resistant Enterococcus spp. and Enterobacteriaceae in food-producing animals. The aim of this work was to evaluate the effect of oxytetracycline or enrofloxacin treatment on vancomycin-resistant enterococci (VRE), extended spectrum β-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae in pigs. A total of 26 piglets were received and distributed in three groups. Group 1 was treated with enrofloxacin (N = 12), group 2 with oxytetracycline (N = 10) and group 3 did not receive any treatment (control group) (N = 4). A higher number of vancomycin-resistant E. faecium were recovered compared to E. faecalis. In the pigs treated with enrofloxacin, vancomycin resistant E. faecium was found in a higher percentage of animals than in the control group. ESBL-producing E. coli was not detected in rectal samples from control animals. However, it was detected in 17–20% of animals treated with oxytetracycline on days 6 to 17 and in 17–50% of the animals treated with enrofloxacin. Carbapenemase-producing E. coli was isolated in animals treated with oxytetracycline, but not in animals treated with enrofloxacin or in the control group. This study highlights that the use of oxytetracycline or enrofloxacin in food-producing animals could select ESBL and carbapenemase-producing E. coli. Further studies shall be needed to validate the results obtained, considering a more robust and extended experimental design.
Performance of Different Echocardiographic Measurements of Left Atrial Size in Dogs by Observers with Different Levels of Experience
Authors: Safian Alexander M. , Menciotti Giulio , Lahmers Sunshine M. , Jeong Hyeon , Franchini Alessandra , Borgarelli Michele
Published: March 2022

Assessment of left atrial (LA) sizes in dogs informs clinical staging, risk assessment, treatment decisions, and prognosis. The objective of this study was to assess the diagnostic performance of observers with different levels of experience measuring the LA with three different techniques. Echocardiographic images from 36 dogs with different degrees of left atrial enlargement (LAE) were retrospectively retrieved, anonymized and measured in a blinded fashion by a veterinary student, a first-year cardiology resident, a third-year cardiology resident, and two board-certified veterinary cardiologists. The LA to aortic root ratio (LA:Ao), LA antero-postero diameter indexed to body weight (LAiAPD) and left atrial area were measured. Inter- and intra-observer intraclass correlation coefficients (ICCs) were calculated for all three variables. Bland–Altman plots and accuracy in identification of LAE were calculated for the three least experienced observers using LA:Ao and LAiAPD. Intra- and interobserver ICCs were greater than 0.9 for every variable. The observer with least experience had significant positive bias and a tendency to overestimate larger measurements using LA:Ao, but not using LAiAPD. The accuracy of identification of LAE also increased with the increasing level of experience and was higher for LAiAPD compared to LA:Ao. Combining both methods for identification of LAE, further increased accuracy.
Association between Increased the De Ritis Quotient and Renal Azotaemia in Canine Babesiosis
Authors: Gójska-Zygner Olga , Karabowicz Justyna , Bartosik Justyna , Zygner Wojciech
Published: March 2022

Previous studies of azotaemia in canine babesiosis revealed pre-renal and renal azotaemia in infected dogs, and an association between an increased de Ritis quotient (aspartate aminotransferase to alanine aminotransferase activity; AST/ALT ratio) and azotaemia in affected animals. Serum activities of AST and ALT, and AST/ALT ratio were compared between azotaemic and non-azotaemic dogs infected with Babesia canis, and between affected dogs with pre-renal and renal azotaemia. Statistical analyses revealed higher AST activity and an increased AST/ALT ratio in azotaemic dogs, and an increase of these two parameters in infected dogs with renal azotaemia in comparison to dogs with pre-renal azotaemia. Moreover, AST activity and AST/ALT ratio were correlated with renal indices such as renal failure index, sodium fractional excretion, and urinary creatinine to serum creatinine ratio. The study also revealed a lack of correlation between AST and ALT activities in azotaemic dogs, although a correlation was observed when including all dogs in this study (azotaemic and non-azotaemic dogs treated as one group). The results of this study indicate that increased serum AST activity in azotaemic dogs infected with B. canis may have a renal origin, and the AST/ALT ratio could be considered as a simple and convenient renal index that is useful in the recognition of renal azotaemia in canine babesiosis.
Effects of In Ovo Injection of Zinc or Diet Supplementation of Zinc on Performance, Serum Biochemical Profiles, and Meat Quality in Broilers
Authors: Kim Hee-Jin , Kang Hwan-Ku
Published: March 2022

This study investigated the main effects of the in ovo injection of inorganic zinc (Zn) or diet supplementation of Zn on performance, serum biochemical profiles, and breast meat quality in broilers. A total of 480 one-day-old broilers (Ross 308) were randomly divided into four groups: the control (Con, noninjected and basal diet), in ovo (injected 60 mg Zn/egg at 18 embryonic days of incubation and basal diet), Zn100 (noninjected and basal diet with Zn (100 mg/kg) for 35 days), and Zn200 (noninjected and basal diet with Zn (200 mg/kg) for 35 days) groups. The dietary supplementation of Zn increased feed intake (2860.42–2861.08 g), weight (1975.06–1985.25 g), and weight gain (1936.36–1946.53 g) compared to Con (2785.74, 1891.38, and 1852.62 g, respectively) after five weeks of age. No significant difference was found in biochemical parameters and leukocyte and erythrocyte levels in the blood among the four different groups. In ovo injected or supplemental Zn (100 and 200 mg/kg) increased IgG in the blood of broilers. Zn200 increased polyunsaturated fatty acids, and saturated fatty acid contents were reduced in breast meat compared with Con. In conclusion, Zn supplementation at 200 mg/kg could improve the weight, feed intake, blood immune response, and fatty acid profile of breast meat.
Investigation of the Quality of the 12 Most-Used Antibiotics Available in Retail Private Pharmacies in Rwanda
Authors: Bizimana Thomas , Kagisha Védaste , Nyandwi Jean Baptiste , Nyirimigabo Alain Katembezi , Muganga Raymond , Mukanyangezi Marie Françoise , Kayitare Egide
Published: March 2022

Using poor-quality antibiotics leads to increased risk of the development of microorganism-resistant strains, treatment failure, loss of confidence in health systems, and associated socio-economic impacts. The prevalence of poor-quality antibiotics has been found to be high in some of the Low and Middle-Income Countries (LMICs), but no data were available on the situation in Rwanda. This study was conducted to obtain data and inform health professionals on the quality of the 12 most-used selected antibiotics from private retail pharmacies in Rwanda. The investigation was conducted on 232 batches collected from randomly selected private retail pharmacies in all provinces of Rwanda, and concerned only with visual inspection and assay tests. Visual inspection was performed using a tool adopted by the International Pharmaceutical Federation (FIP) to identify manufacturing defects. An assay test quantified the Active Pharmaceutical Ingredient (API) in each collected batch using high-performance liquid chromatography (HPLC) coupled with an ultraviolet-visible (UV) detector, and the results were reported as the percentage content of the amount of APIs stated on the label. A total of 232 batches were analyzed, manufactured in 10 countries; the main country of manufacture was Kenya, with almost half of the batches (49.6%). The results of the visual inspection did not show the presence of counterfeit/ falsified antibiotics on the Rwandan market in this study but revealed weaknesses in labeling: more than 90% of the analyzed batches of the 12 antibiotics did not present the dosage statement on their label, and the complete list of excipients was missing in more than 20% of the analyzed batches. The assay test using HPLC confirmed the presence of APIs in 100% of the analyzed batches. However, moderate deviations from acceptable ranges of the API content defined by M. M. Nasr & C. M. Stanley in 2006 for erythromycin and the United States Pharmacopoeia 2018 for the other 11 molecules were found. The failure rate to meet the quality requirements in terms of the percentage content of active pharmaceutical ingredients declared on the labels was estimated at 8.2% in total, with 3.9% and 4.3% containing more and less than the amount of APIs stated on the labels respectively. The most-represented antibiotics on the Rwandan market were amoxicillin, co-trimoxazole and cloxacillin. No counterfeit antibiotics were found in this study. However, substandard batches with moderate deviations were found, suggesting that regular quality control of antibiotics is needed in Rwanda.
Application of the Theory of Planned Behavior in Autonomous Vehicle-Pedestrian Interaction
Authors: Hafeez Farrukh , Ullah Sheikh Usman , Mas’ud Abdullahi Abubakar , Al-Shammari Saud , Hamid Muhammad , Azhar Ameer
Published: March 2022

Automobile manufacturers, alongside technology providers, researchers, and public agencies, are conducting extensive testing to design autonomous vehicles (AVs) algorithms that will provide a complete understanding of road users, specifically pedestrians. Pedestrian behavior and actions determination are highly unpredictable depending on behavioral beliefs, context, and socio-demographic variables. Context includes everything that potentially affects one’s behavior; in AVs–pedestrian interaction, context may consist of weather conditions, road structure, social factors norms, and traffic volume. These influencing elements, therefore, need to be focused on during the development of pedestrian interaction algorithms. For this purpose, the pedestrian behavior questionnaire for FAVs (PBQF) is designed based on the theory of planned behavior (TPB). A total of almost 1000 voluntary participants completed this multilingual survey. As socio-demographic values and physiological perception varies with local norms, regions, and ethnicity, participants from 27 countries were therefore chosen to account for this variation. One of the key findings of this study is the influence of pedestrian attributes and the context on pedestrian behavior. Pedestrian action cannot be understood without visual observation of the pedestrian themselves and their context. The findings showed that pedestrians build communication with vehicles based on their driving styles. The vehicle’s driving style leads pedestrians to think that the vehicle is human-driven or autonomous. The results also revealed that pedestrians use several cues to show their intention. The general perception of AVs was also analyzed, and the communication between AVs and pedestrians with different displaying options was investigated.
Study on Contamination with Some Mycotoxins in Maize and Maize-Derived Foods
Authors: Mihalcea Alina , Amariei Sonia
Published: March 2022

Crops can be contaminated by fungi which produce mycotoxins. Many fungal strains are responsible for producing varied mycotoxins. The research carried out so far has described over 400 different mycotoxins. They have chemical and physical properties that significantly differ, and they are produced by several different existing fungi. The intake of mycotoxins through food can be achieved directly, by feeding on contaminated food, or indirectly from foods of animal origin. The mycotoxin contamination of food and food products for certain animals is a phenomenon studied worldwide, in countries in Europe but also in Asia, Africa and America. The purpose of this study is to develop an evaluation of the mycotoxins prevalent in corn and corn-derived products produced in Romania. A total of 38 maize samples and 19 corn-derivative samples were investigated for the presence of mycotoxins specific to these products, such as deoxynivalenol, zearalenone and fumonisins. Fumonisins had the highest presence and zearalenone had the lowest. The limits determined for the three mycotoxins were always in accordance with legal regulations.
Non-Targeted NMR Method to Assess the Authenticity of Saffron and Trace the Agronomic Practices Applied for Its Production
Authors: Musio Biagia , Todisco Stefano , Antonicelli Marica , Garino Cristiano , Arlorio Marco , Mastrorilli Piero , Latronico Mario , Gallo Vito
Published: March 2022

The development of analytical methods aimed at tracing agri-food products and assessing their authenticity is essential to protect food commercial value and human health. An NMR-based non-targeted method is applied here to establish the authenticity of saffron samples. Specifically, 40 authentic saffron samples were compared with 18 samples intentionally adulterated by using turmeric and safflower at three different concentration levels, i.e., 5, 10, and 20 wt%. Statistical processing of NMR data furnished useful information about the main biomarkers contained in aqueous and dimethyl sulfoxide extracts, which are indicative of the presence of adulterants within the analyzed matrix. Furthermore, a discrimination model was developed capable of revealing the type of agronomic practice adopted during the production of this precious spice, distinguishing between organic and conventional cultivation. The main objective of this work was to provide the scientific community involved in the quality control of agri-food products with an analytical methodology able to extract useful information quickly and reliably for traceability and authenticity purposes. The proposed methodology turned out to be sensitive to minor variations in the metabolic composition of saffron that occur in the presence of the two adulterants studied. Both adulterants can be detected in aqueous extracts at a concentration of 5 wt%. A lower limit of detection was observed for safflower contained in organic extracts in which case the lowest detectable concentration was 20%.
Design of a Chaotic Trajectory Generator Algorithm for Mobile Robots
Authors: Cetina-Denis Juan José , Lopéz-Gutiérrez Rosa Martha , Cruz-Hernández César , Arellano-Delgado Adrian
Published: March 2022

This paper addresses the problem of designing an algorithm capable of generating chaotic trajectories for mobile robots. In particular, the chaotic behavior is induced in the linear and angular velocities of a differential drive mobile robot by infusing them with the states of the Hénon chaotic map. A possible application of patrolling a work area by using trajectories with chaotic motion in differential drive mobile robots is presented. In this work, obtained numerical and experimental results are reported and analyzed. In addition, two quantitative numerical tests are applied in order to measure the chaoticity of the generated trajectories. With the intention of expanding this work further, we explore the case where multiple mobile robots move simultaneously in the same work area with different trajectories, and the final results are exhaustively analyzed. In addition, experimental results are presented.
Special Issue on Cryptography and Its Applications in Information Security
Authors: El Assad Safwan , Lozi René , Puech William
Published: March 2022

Nowadays, mankind is living in a cyber world [...]
Design of a Dead-Time Compensator Robust H∞ State Feedback Temperature Controller for a Precalciner of a Cement Rotary Kiln
Authors: Salcedo-Hernández José , Rivas-Perez Raul , Sotomayor-Moriano Javier
Published: March 2022

A dead-time compensator robust H∞ state feedback controller (DTC-H∞-SFC) for the temperature control in a precalciner of a cement rotary kiln is designed. A mathematical model of the process under study with ARMAX structure was obtained. A dead-time compensator robust H∞ state feedback controller is therefore designed. The results of the comparative evaluation of the DTC-H∞-SFC vs. DTC+PI designed controllers showed that the DTC-H∞-SFC gives improved performance of the control system.
Heart Failure Patients’ Adherence to Hybrid Comprehensive Telerehabilitation and Its Impact on Prognosis Based on Data from TELEREH-HF Randomized Clinical Trial
Authors: Piotrowicz Ewa , Pencina Michael , Opolski Grzegorz , Zaręba Wojciech , Banach Maciej , Kowalik Ilona , Orzechowski Piotr , Główczyńska Renata , Szalewska Dominika , Pluta Sławomir , Kalarus Zbigniew , Irzmański Robert , Piotrowicz Ryszard
Published: March 2022

(1) Background Adherence to treatment guidelines in heart failure (HF) patients is of major prognostic importance, but thorough implementation of guidelines in routine care remains insufficient. Introducing hybrid comprehensive telerehabilitation (HCTR) consisting of telecare, telerehabilitation, and remote monitoring of implantable devices might be an option to improve adherence to recommendation and can affect the prognosis. The purpose is to investigate the association of adherence to HCTR with mortality and hospitalization. (2) Methods This analysis formed part of TELEREH-HF multi-center, randomized trial that enrolled 850HF patients (NYHA I-III;LVEF ≤ 40%). Patients were randomized 1:1 to 9-week HCTR (1 week in hospital and 8 weeks at home) plus usual care or usual care only and followed-up for 14 to 26 months. This analysis focuses on the HCTR group. Adherent patients were those who adhered both to the number of training sessions prescribed and to the duration of the prescribed cycle by at least 80%; non-adherent patients were those who adhered<20% to the prescribed number of training sessions and their duration. The remaining patients were classified as partially adherent. (3) Results There were 350 (88.4%) adherent patients, 39 (9.8%) partially adherent patients, and 7 (1.8%) non-adherent patients. There were 46 deaths during follow-up. Non-adherence or partial adherence was associated with higher risk of cardiovascular (CV) mortality (hazard ratio (HR) = 2.62, p = 0.021); all-cause mortality or HF hospitalization (HR = 1.71, p = 0.038); CV mortality or HF hospitalization (HR = 1.89, p = 0.014). (4) Conclusions The adherence to HCTR was high. Adherence to HCTR was associated with improved prognosis for CV mortality and the reduction in the combined outcome of CV mortality or HF hospitalization.
Mechanistic–Empirical Analysis of Pavement Performance Considering Dynamic Axle Load Spectra Due to Longitudinal Unevenness
Authors: Zhao Jingnan , Wang Hao , Lu Pan , Chen Jiaqi
Published: March 2022

Pavement distresses are induced by mechanistic responses in pavement structure subjected to dynamic loads of moving vehicles. Pavement surface evenness deteriorates as pavement distresses propagate, which results in dynamic axle loads and faster pavement deterioration. It is vital to consider the dynamic axle load spectra to predict pavement deterioration using traffic-monitoring data. This study aimed to evaluate the effect of dynamic loads and overweight traffic on asphalt pavement overlay performance using mechanistic–empirical (M–E) pavement analysis. The relationship between dynamic load coefficients (DLCs), axle loads, and international roughness index (IRI) was obtained for accurate quantification of dynamic axle loads. Then the dynamic axle load spectra were derived by shifting the static axle load spectra in weigh-in-motion (WIM) data, given the DLC value. AASHTOWare Pavement ME software was used to analyze pavement performance with static and dynamic axle load spectra, and the impact of overweight traffic on asphalt pavement overlay performance. The impact of dynamic loads on reflective fatigue cracking was distinguished at an early stage of the service period and eliminated after the 10-year analysis period, when the propagation of reflective cracking reached a specific level. On the other hand, the consideration of dynamic axle loads increased the impact of overweight truck traffic on pavement distresses, and pavement structures of major highways tend to be more sensitive to overweight traffic because of greater DLC excitement at higher operational speeds.
Projected Changes in the East Asian Hydrological Cycle for Different Levels of Future Global Warming
Authors: Chevuturi Amulya , Klingaman Nicholas P. , Turner Andrew G. , Guo Liang , Vidale Pier Luigi
Published: March 2022

Recent decades have shown significant changes to the hydrological cycle over East Asia (EA), and further changes are expected due to future global warming. This study evaluates projected seasonal changes in the EA hydrological cycle using simulations that are 1.5 °C, 2.0 °C and 3.0 ∘C warmer than pre-industrial, from the Met Office Unified Model (MetUM) Global Ocean Mixed Layer model version 2.0 (GOML2.0), compared against present-day conditions. The moisture sources of the warming-induced precipitation changes are identified over five hydrologically unique regions within EA. Precipitation over EA increases with warming (except over southeastern EA in the spring and autumn) due to the intensified hydrological cycle. The projected seasonal changes in the hydrological cycle are usually nonlinear, with the rate of change between 1.5 ∘C and 2.0 ∘C larger than the rate of change between 2.0 ∘C and 3.0 ∘C of warming. The warming-induced precipitation increases are mainly associated with an increase in remote moisture convergence rather than local moisture recycling, except over the Tibetan Plateau. Decomposition of the changes in moisture sources by direction and flux component indicate that changes from the west are dominated by changes to moisture and changes from the north are more circulation driven. The changes from the south are moisture driven over southern EA and driven by moisture and circulation change over northern EA. Our results highlight the regionally and seasonally diverse projected changes to the EA hydrological cycle due to global warming, which will be useful for region-specific climate mitigation policies and the implementation of seasonally varying adaptation methods.
Exploring Health Impacts of Occupational Exposure to Carbon Monoxide in the Labour Community of Hattar Industrial Estate
Authors: Jadoon Suneela , Nawazish Shamyla , Mahmood Qaisar , Rafique Asif , Sohail Summar , Zaidi Asma
Published: March 2022

This study was designed to assess the health impacts related to noninvasive carbon monoxide saturation (SPCO %) in the blood of respondents. For this purpose, 150 respondents from the labour community of Hattar Industrial Estate (testing site) and 100 respondents from Sultan Pur (control site) were selected. To achieve this objective, a Rad-57 Pulse CO-Oximeter was used for noninvasive carboxyhemoglobin measurement. Carbon monoxide saturation (SPCO%) in the blood of respondents from Hattar Industrial Estate, Haripur, Pakistan has been compared with the WHO’s standard concentration of SPCO% (5%). High saturation of carbon monoxide (carboxyhemoglobin SPCO) in the blood of respondents and disease association have been interpreted in graphs formed on the basis of statistical analysis in terms of frequencies, using statistical software (SPSS), based on demographic entries as well as exposure time of the employees in the processing, food and steel industries. The highest SPCO% measured was 17% in the steel industry and the lowest measured level was 4.2%. Frequencies and percentages of respiratory inflammation, dermatosis, asthma, breathing issues and eye inflammation among respondents were 29%, 35%, 16.7%, 23.5% and 9%, respectively. Prevalence of disease in three different groups of respondents (from three testing sites) was also analyzed on the basis of exposure time (hrs.) to carbon monoxide emissions. Prevalence of disease among the exposed and non-exposed groups was analyzed and showed comparatively lower disease prevalence in the group of respondents who were not exposed to high carbon monoxide emissions. The data of the current study was also subjected to statistical modelling to find the health risk of air pollutants (carbon monoxide) on population health by calculating attributable risk (AR) or attributable proportion (AP). Results indicated that attributable risk of carbon monoxide exposure for respiratory diseases, dermatosis and eye inflammation were 61.12%, 65.77% and 24.95% respectively. Findings of statistical modelling indicated that dermatosis and respiratory diseases were more prevalent in laborers of industrial units than those at control site.
Cone-Beam Computed Tomographic Assessment of the Mandibular Condylar Volume in Different Skeletal Patterns: A Retrospective Study in Adult Patients
Authors: Ceratti Chiara , Maspero Cinzia , Consonni Dario , Caprioglio Alberto , Connelly Stephen Thaddeus , Inchingolo Francesco , Tartaglia Gianluca Martino
Published: March 2022

The aim of this study was to assess the condylar volume in adult patients with different skeletal classes and vertical patterns using cone-beam computed tomography (CBCT). CBCT scans of 146 condyles from 73 patients (mean age 30 ± 12 years old; 49 female, 24 male) were selected from the archive of the Department of Dentistry and Maxillofacial Surgery of Fondazione IRCCS Ca’ Granda, Milan, Italy, and retrospectively analyzed. The following inclusion criteria were used: adult patients; CBCT performed with the same protocol (0.4 mm slice thickness, 16 × 22 cm field of view, 20 s scan time); no systemic diseases; and no previous orthodontic treatments. Three-dimensional cephalometric tracings were performed for each patient, the mandibular condyles were segmented and the relevant volumes calculated using Mimics Materialize 20.0® software (Materialise, Leuven, Belgium). Right and left variables were analyzed together using random-intercept linear regression models. No significant association between condylar volumes and skeletal class was found. On the other hand, in relation to vertical patterns, the mean values of the mandibular condyle volumes in hyperdivergent subjects (688 mm3) with a post-rotation growth pattern (625 mm3) were smaller than in hypodivergent patients (812 mm3) with a horizontal growth pattern (900 mm3). Patients with an increased divergence angle had smaller condylar volumes than subjects with normal or decreased mandibular plane divergence. This relationship may help the clinician when planning orthodontic treatment.
EXOC6 (Exocyst Complex Component 6) Is Associated with the Risk of Type 2 Diabetes and Pancreatic β-Cell Dysfunction
Authors: Sulaiman Nabil , Yaseen Hachim Mahmood , Khalique Anila , Mohammed Abdul Khader , Al Heialy Saba , Taneera Jalal
Published: March 2022

EXOC6 and EXOC6B (EXOC6/6B) components of the exocyst complex are involved in the secretory granule docking. Recently, EXOC6/6B were anticipated as a molecular link between dysfunctional pancreatic islets and ciliated lung epithelium, making diabetic patients more prone to severe SARS-CoV-2 complications. However, the exact role of EXOC6/6B in pancreatic β-cell function and risk of T2D is not fully understood. Herein, microarray and RNA-sequencing (RNA-seq) expression data demonstrated the expression of EXOC6/6B in human pancreatic islets. Expression of EXOC6/6B was not affected by diabetes status. Exploration of the using the translational human pancreatic islet genotype tissue-expression resource portal (TIGER) revealed three genetic variants (rs947591, rs2488071 and rs2488073) in the EXOC6 gene that were associated (p < 2.5 × 10−20) with the risk of T2D. Exoc6/6b silencing in rat pancreatic β-cells (INS1-832/13) impaired insulin secretion, insulin content, exocytosis machinery and glucose uptake without cytotoxic effect. A significant decrease in the expression Ins1, Ins1, Pdx1, Glut2 and Vamp2 was observed in Exoc6/6b-silenced cells at the mRNA and protein levels. However, NeuroD1, Gck and InsR were not influenced compared to the negative control. In conclusion, our data propose that EXOC6/6B are crucial regulators for insulin secretion and exocytosis machinery in β-cells. This study identified several genetic variants in EXOC6 associated with the risk of T2D. Therefore, EXOC6/6B could provide a new potential target for therapy development or early biomarkers for T2D.
Optimal Biomechanical Performance in Upper-Limb Gestures Depends on Velocity and Carried Load
Authors: Brambilla Cristina , Malosio Matteo , Reni Gianluigi , Scano Alessandro
Published: March 2022

In the last few years, there has been increased interest in the preservation of physical and mental health of workers that cooperate with robots in industrial contexts, such as in the framework of the European H2020 Mindbot Project. Since biomechanical analysis contributes to the characterization of the subject interacting with a robotic setup and platform, we tested different speed and loading conditions in a simulated environment to determine upper-limb optimal performance. The simulations were performed starting from laboratory data of people executing upper-limb frontal reaching movements, by scaling the motion law and imposing various carried loads at the hand. The simulated velocity ranged from 20% to 200% of the original natural speed, with step increments of 10%, while the hand loads were 0, 0.5, 1, and 2 kg, simulating carried objects. A 3D inverse kinematic and dynamic model was used to compute upper-limb kinematics and dynamics, including shoulder flexion, shoulder abduction, and elbow flexion. An optimal range of velocities was found in which the expended energy was lower. Interestingly, the optimal speed corresponding to lower exerted torque and energy decreased when the load applied increased. Lastly, we introduced a preliminary movement inefficiency index to evaluate the deviation of the power and expended energy for the shoulder flexion degree of freedom when not coinciding with the minimum energy condition. These results can be useful in human–robot collaboration to design minimum-fatigue collaborative tasks, tune setup parameters and robot behavior, and support physical and mental health for workers.
Influences of Serendipita indica and Dictyophorae echinovolvata on the Growth and Fusarium Wilt Disease Resistance of Banana
Authors: Cheng Chunzhen , Liu Fan , Wang Bin , Qu Pengyan , Liu Jiapeng , Zhang Yongyan , Liu Wei , Tong Zheng , Deng Guiming
Published: March 2022

Recently, many control methods have been tried and applied in the Fusarium wilt disease control of banana and have achieved definite progresses. In this study, by using ‘Zhongjiao No.3’ and ‘Zhongjiao No.4’ banana seedlings as materials, the effects of Serendipita indica and bamboo fungus (Dictyophorae echinovolvata) culture substrates on the growth and Fusarium wilt disease resistance of banana were investigated. Results showed that the plant height, leaf length, leaf width, root length and root thickness, aboveground part fresh weight, root fresh weight, and relative chlorophyll content and nitrogen content in leaves of banana seedlings colonized with S. indica were all greater than those of non-colonized controls, while these parameters of banana seedlings grown in nutrient soil containing D. echinovolvata culture substrates were significantly suppressed. Both S. indica non-colonized and colonized seedlings cultivated in nutrient containing 1/4 D. echinovolvata culture substrates showed much milder symptoms compared with those cultivated in normal nutrient soil, indicating that the addition of bamboo fungus substrates to the soil can enhance the Fusarium wilt resistance of banana. The results obtained in this study can provide a basis for the application of S. indica and bamboo fungus in the prevention and control of banana Fusarium wilt disease.
Desmopressin Stimulates Nitric Oxide Production in Human Lung Microvascular Endothelial Cells
Authors: Rotoli Bianca Maria , Visigalli Rossana , Ferrari Francesca , Ranieri Marianna , Tamma Grazia , Dall’Asta Valeria , Barilli Amelia
Published: March 2022

Desmopressin (dDAVP) is the best characterized analogue of vasopressin, the endocrine regulator of water balance endowed with potent vasoconstrictive effects. Despite the use of dDAVP in clinical practice, ranging from the treatment of nephrogenic diabetes insipidus to bleeding disorders, much remains to be understood about the impact of the drug on endothelial phenotype. The aim of this study was, thus, to evaluate the effects of desmopressin on the viability and function of human pulmonary microvascular endothelial cells (HLMVECs). The results obtained demonstrate that the vasopressor had no cytotoxic effect on the endothelium; similarly, no sign of endothelial activation was induced by dDAVP, indicated by the lack of effect on the expression of inflammatory cytokines and adhesion molecules. Conversely, the drug significantly stimulated the production of nitric oxide (NO) and the expression of the inducible isoform of nitric oxide synthase, NOS2/iNOS. Since the intracellular level of cAMP also increased, we can hypothesize that NO release is consequent to the activation of the vasopressin receptor 2 (V2R)/guanylate cyclase (Gs)/cAMP axis. Given the multifaceted role of NOS2-deriving NO for many physio-pathological conditions, the meanings of these findings in HLMVECs appears intriguing and deserves to be further addressed.
Bandages Static Stiffness Index Is Not Influenced by Calf Mechanical Properties but Only by Geometrical Changes
Authors: Chassagne Fanette , Molimard Jérôme , Convert Reynald , Helouin-Desenne Clothilde , Badel Pierre , Giraux Pascal
Published: March 2022

Interface pressure applied by compression bandages is the therapeutic action of the treatment of some venous or lymphatic pathologies. The so-called Static Stiffness Index, which quantifies the pressure increase from supine to standing position, is usually used to differentiate compression bandages. It was hypothesized that this pressure increase was the consequence of a change in leg geometry (blood and muscle falling down) and a change in calf soft tissue mechanical properties (muscles contraction). Calf soft tissue global stiffness of both legs of 25 patients was characterized in a sitting and standing position. This characterization was combined with interface pressure measurements applied by six different bandages. Though soft tissue mechanical properties significantly increased from sitting to standing position, no correlation was observed with the corresponding pressure increase. Thus, pressure increase is mainly attributed to a change in leg geometry.
Poxviruses Bearing DNA Polymerase Mutations Show Complex Patterns of Cross-Resistance
Authors: Andrei Graciela , Fiten Pierre , Krečmerová Marcela , Opdenakker Ghislain , Topalis Dimitrios , Snoeck Robert
Published: March 2022

Despite the eradication of smallpox four decades ago, poxviruses continue to be a threat to humans and animals. The arsenal of anti-poxvirus agents is very limited and understanding mechanisms of resistance to agents targeting viral DNA polymerases is fundamental for the development of antiviral therapies. We describe here the phenotypic and genotypic characterization of poxvirus DNA polymerase mutants isolated under selective pressure with different acyclic nucleoside phosphonates, including HPMPC (cidofovir), cHPMPC, HPMPA, cHPMPA, HPMPDAP, HPMPO-DAPy, and PMEO-DAPy, and the pyrophosphate analogue phosphonoacetic acid. Vaccinia virus (VACV) and cowpox virus drug-resistant viral clones emerging under drug pressure were characterized phenotypically (drug-susceptibility profile) and genotypically (DNA polymerase sequencing). Different amino acid changes in the polymerase domain and in the 3′-5′ exonuclease domain were linked to drug resistance. Changes in the 3′-5′ domain emerged earlier than in the polymerase domain when viruses acquired a combination of mutations. Our study highlights the importance of poxvirus DNA polymerase residues 314, 613, 684, 688, and 851, previously linked to drug resistance, and identified several novel mutations in the 3′-5′ exonuclease domain (M313I, F354L, D480Y) and in the DNA polymerase domain (A632T, T831I, E856K, L924F) associated with different drug-susceptibility profiles. Furthermore, a combination of mutations resulted in complex patterns of cross-resistance. Modeling of the VACV DNA polymerase bearing the newly described mutations was performed to understand the effects of these mutations on the structure of the viral enzyme. We demonstrated the emergence of drug-resistant DNA polymerase mutations in complex patterns to be considered in case such mutations should eventually arise in the clinic.
Urinary Galectin-3 as a Novel Biomarker for the Prediction of Renal Fibrosis and Kidney Disease Progression
Authors: Ou Shuo-Ming , Tsai Ming-Tsun , Chen Huan-Yuan , Li Fu-An , Lee Kuo-Hua , Tseng Wei-Cheng , Chang Fu-Pang , Lin Yao-Ping , Yang Ruey-Bing , Tarng Der-Cherng
Published: March 2022

Plasma galectin-3 (Gal-3) is associated with organ fibrosis, but whether urinary Gal-3 is a potential biomarker of kidney disease progression has never been explored. Between 2018 and 2021, we prospectively enrolled 280 patients who underwent renal biopsy and were divided into three groups based on their urinary Gal-3 levels (<354.6, 354.6–510.7, and ≥510.8 pg/mL) to assess kidney disease progression (defined as ≥40% decline in the estimated glomerular filtration rate or end-stage renal disease) and renal histology findings. Patients in the highest urinary Gal-3 tertile had the lowest eGFRs and highest proteinuria levels. In multivariate Cox regression models, patients in the highest tertile had the highest risk of kidney disease progression (adjusted hazard ratio, 4.60; 95% confidence interval, 2.85–7.71) compared to those in the lowest tertile. Higher urinary Gal-3 levels were associated with more severe renal fibrosis. Intrarenal mRNA expression of LGALS3 (Gal-3-encoded gene) was most correlated with the renal stress biomarkers (IGFBP7 and TIMB2), renal function biomarkers (PTGDS) and fibrosis-associated genes (TGFB1). The urinary Gal-3 level may be useful for the identification of patients at high risk of kidney disease progression and renal fibrosis, and for the early initiation of treatments for these patients.
Clinical Applications of Cancer-Associated Cells Present in the Blood of Cancer Patients
Authors: Tang Cha-Mei , Adams Daniel L.
Published: March 2022

The ability to obtain tumor material from cells in the blood of cancer patients provides a significant benefit over the use of tumor tissue as a diagnostic to make treatment decisions. However, the traditionally defined circulating tumor cell (CTC) has been shown to be useful only in some cases. A recently identified type of circulating stromal cell, which appears to be more frequent than CTCs, was found engulfing tumor material at the tumor site and then entering the blood stream. These cells were defined as cancer-associated macrophage-like cells (CAMLs). Together, CTCs and CAMLs may be able to provide information for cancer detection and diagnosis, without the use of tissue. CTCs and CAMLs have many clinical applications, three of which are summarized in this review: for prognosis, as companion diagnostics, and for residual disease monitoring.
Immunocapture Magnetic Beads Enhanced the LAMP-CRISPR/Cas12a Method for the Sensitive, Specific, and Visual Detection of Campylobacter jejuni
Authors: Li Chao , Chen Xuan , Wen Renqiao , Ma Peng , Gu Kui , Li Cui , Zhou Changyu , Lei Changwei , Tang Yizhi , Wang Hongning
Published: March 2022

Campylobacter jejuni is one of the most important causes of food-borne infectious disease, and poses challenges to food safety and public health. Establishing a rapid, accurate, sensitive, and simple detection method for C. jejuni enables early diagnosis, early intervention, and prevention of pathogen transmission. In this study, an immunocapture magnetic bead (ICB)-enhanced loop-mediated isothermal amplification (LAMP) CRISPR/Cas12a method (ICB-LAMP-CRISPR/Cas12a) was developed for the rapid and visual detection of C. jejuni. Using the ICB-LAMP-CRISPR/Cas12a method, C. jejuni was first captured by ICB, and the bacterial genomic DNA was then released by heating and used in the LAMP reaction. After the LAMP reaction, LAMP products were mixed and detected by the CRISPR/Cas12a cleavage mixture. This ICB-LAMP-CRISPR/Cas12a method could detect a minimum of 8 CFU/mL of C. jejuni within 70 min. Additionally, the method was performed in a closed tube in addition to ICB capture, which eliminates the need to separate preamplification and transfer of amplified products to avoid aerosol pollution. The ICB-LAMP-CRISPR/Cas12a method was further validated by testing 31 C. jejuni-positive fecal samples from different layer farms. This method is an all-in-one, simple, rapid, ultrasensitive, ultraspecific, visual detection method for instrument-free diagnosis of C. jejuni, and has wide application potential in future work.
Automatic and Accurate Sleep Stage Classification via a Convolutional Deep Neural Network and Nanomembrane Electrodes
Authors: Kwon Kangkyu , Kwon Shinjae , Yeo Woon-Hong
Published: March 2022

Sleep stage classification is an essential process of diagnosing sleep disorders and related diseases. Automatic sleep stage classification using machine learning has been widely studied due to its higher efficiency compared with manual scoring. Typically, a few polysomnography data are selected as input signals, and human experts label the corresponding sleep stages manually. However, the manual process includes human error and inconsistency in the scoring and stage classification. Here, we present a convolutional neural network (CNN)-based classification method that offers highly accurate, automatic sleep stage detection, validated by a public dataset and new data measured by wearable nanomembrane dry electrodes. First, our study makes a training and validation model using a public dataset with two brain signal and two eye signal channels. Then, we validate this model with a new dataset measured by a set of nanomembrane electrodes. The result of the automatic sleep stage classification shows that our CNN model with multi-taper spectrogram pre-processing achieved 88.85% training accuracy on the validation dataset and 81.52% prediction accuracy on our laboratory dataset. These results validate the reliability of our classification method on the standard polysomnography dataset and the transferability of our CNN model for other datasets measured with the wearable electrodes.
Droplet Manipulation under a Magnetic Field: A Review
Authors: Zhu Gui-Ping , Wang Qi-Yue , Ma Zhao-Kun , Wu Shi-Hua , Guo Yi-Pan
Published: March 2022

The magnetic manipulation of droplets is one of the emerging magnetofluidic technologies that integrate multiple disciplines, such as electromagnetics, fluid mechanics and so on. The directly driven droplets are mainly composed of ferrofluid or liquid metal. This kind of magnetically induced droplet manipulation provides a remote, wireless and programmable approach beneficial for research and engineering applications, such as drug synthesis, biochemistry, sample preparation in life sciences, biomedicine, tissue engineering, etc. Based on the significant growth in the study of magneto droplet handling achieved over the past decades, further and more profound explorations in this field gained impetus, raising concentrations on the construction of a comprehensive working mechanism and the commercialization of this technology. Current challenges faced are not limited to the design and fabrication of the magnetic field, the material, the acquisition of precise and stable droplet performance, other constraints in processing speed and so on. The rotational devices or systems could give rise to additional issues on bulky appearance, high cost, low reliability, etc. Various magnetically introduced droplet behaviors, such as deformation, displacement, rotation, levitation, splitting and fusion, are mainly introduced in this work, involving the basic theory, functions and working principles.
Portable FRET-Based Biosensor Device for On-Site Lead Detection
Authors: Lai Wei-Qun , Chang Yu-Fen , Chou Fang-Ning , Yang De-Ming
Published: March 2022

Most methods for measuring environmental lead (Pb) content are time consuming, expensive, hazardous, and restricted to specific analytical systems. To provide a facile, safe tool to detect Pb, we created pMet-lead, a portable fluorescence resonance energy transfer (FRET)-based Pb-biosensor. The pMet-lead device comprises a 3D-printed frame housing a 405-nm laser diode—an excitation source for fluorescence emission images (YFP and CFP)—accompanied by optical filters, a customized sample holder with a Met-lead 1.44 M1 (the most recent version)-embedded biochip, and an optical lens aligned for smartphone compatibility. Measuring the emission ratios (Y/C) of the FRET components enabled Pb detection with a dynamic range of nearly 2 (1.96), a pMet-lead/Pb dissociation constant (Kd) 45.62 nM, and a limit of detection 24 nM (0.474 μg/dL, 4.74 ppb). To mitigate earlier problems with a lack of selectivity for Pb vs. zinc, we preincubated samples with tricine, a low-affinity zinc chelator. We validated the pMet-lead measurements of the characterized laboratory samples and unknown samples from six regions in Taiwan by inductively coupled plasma mass spectrometry (ICP-MS). Notably, two unknown samples had Y/C ratios significantly higher than that of the control (3.48 ± 0.08 and 3.74 ± 0.12 vs. 2.79 ± 0.02), along with Pb concentrations (10.6 ppb and 15.24 ppb) above the WHO-permitted level of 10 ppb in tap water, while the remaining four unknowns showed no detectable Pb upon ICP-MS. These results demonstrate that pMet-lead provides a rapid, sensitive means for on-site Pb detection in water from the environment and in living/drinking supply systems to prevent potential Pb poisoning.
Computational Modelling of Tone Perception Based on Direct Processing of f0 Contours
Authors: Chen Yue , Gao Yingming , Xu Yi
Published: March 2022

It has been widely assumed that in speech perception it is imperative to first detect a set of distinctive properties or features and then use them to recognize phonetic units like consonants, vowels, and tones. Those features can be auditory cues or articulatory gestures, or a combination of both. There have been no clear demonstrations of how exactly such a two-phase process would work in the perception of continuous speech, however. Here we used computational modelling to explore whether it is possible to recognize phonetic categories from syllable-sized continuous acoustic signals of connected speech without intermediate featural representations. We used Support Vector Machine (SVM) and Self-organizing Map (SOM) to simulate tone perception in Mandarin, by either directly processing f0 trajectories, or extracting various tonal features. The results show that direct tone recognition not only yields better performance than any of the feature extraction schemes, but also requires less computational power. These results suggest that prior extraction of features is unlikely the operational mechanism of speech perception.