bluuebunny/crossref_metadata_2025 huggingface dataset:
Black Soldier Fly Oil in Different Lipid Diets Could Regulate Tissue Lipid Metabolism and Fatty Acid Composition of Juvenile Mirror Carp
Authors: Xu Xinxin , Ji Beibei , Lu Ronghua , Ji Hong
Published: March 2024

In the present study, our aim was to assess the effect of dietary black soldier fly oil (BSFO) with different lipid contents on the growth performance, body composition, lipid metabolism, and related gene expression in juvenile mirror carp (Cyprinus carpio var. specularis). An 8‐week feeding trial was conducted using four isonitrogenous diets (32.0% crude protein) containing two lipid levels: 6% (control (CT) group) and 9% (high lipid (HL) group), both using soybean oil as the oil source. The other two diets contained BSFO on the basis of 6% and 9% lipid, referred to as (CT + BSFO) and (HL + BSFO) groups. The results showed that final body weight, weight gain rate, specific growth rate, protein retention ratio, and feed utilization were significantly increased by using higher dietary lipid levels (P < 0.05). Additionally, the gene expression of lipid synthesis markers was significantly up‐regulated in intra‐peritoneal fat but significantly down‐regulated in the hepatopancreas due to increased dietary lipid level (P < 0.05). No negative effects on feed utilization or growth performance were observed in fish fed diets containing BSFO. However, the intra‐peritoneal fat index, adipocyte size, and hepatopancreas lipid content were significantly decreased in the CT + BSFO group compared to the CT group (P < 0.05). Furthermore, BSFO also up‐regulated the expression of lipid lipolysis markers in the hepatopancreas and intra‐peritoneal fat (P < 0.05). Moreover, the use of BSFO also increased the content of n‐3 polyunsaturated fatty acid (PUFA) while reducing the content of n‐6 PUFA in the muscle (P < 0.05). In conclusion, HL diets improved the growth of the fish and increased the lipid deposition. BSFO in the diet resulted in a reduction of lipid deposition in the hepatopancreas and intra‐peritoneal fat, likely due to an increase in lipid oxidation.
A Collaborative Control Framework: Achieving Emergency Vehicle Priority While Minimizing Negative Impact on Ordinary Vehicles at Signalized Intersection
Authors: Long Ke-jun , Zhang Zhong-gen , Gao Zhi-bo , Zhong Si-qi , Gu Jian
Published: March 2024

When an emergency vehicle (EV) passes through an isolated signal intersection, it is crucial to ensure the efficient passage of the EV while minimizing the negative impact on ordinary vehicles (OVs), particularly in high‐traffic flow scenarios. Given the constraints on temporal and spatial resources within intersection areas, OVs ahead of EV often face challenges in finding safe gaps for giving way, resulting in significant obstructions to OVs. This research introduces a novel collaborative control framework to jointly optimize dynamic emergency lane settings and signal schemes, considering EV priority and OVs benefits for a single signalized intersection. Firstly, we propose a dynamic emergency lane control algorithm to help obstructed EV in roadway segments by extending and reallocating temporal and spatial resources for vehicles. Then, we establish a collaborative control model considering EV priority and OVs benefits. Assigning the highest priority to the emergency priority phase, this model optimizes signal schemes to prevent interphase conflict, taking into account OVs benefits. Finally, our collaborative control framework also employs an Eco‐Driving algorithm for the optimization of OV speed to reduce fuel consumption. The case study results reveal that in comparison to other baseline methods, our proposed model significantly reduces EV travel time, simultaneously lowering the travel time and fuel consumption of OVs. Sensitivity analysis of varying traffic flow scenarios reveals that, as vehicle volumes increase, our proposed method demonstrates more pronounced reductions in both EV and OV travel time. In addition, there is a progressive increase in the proportion of dynamic emergency lane utilization, with activation occurring at earlier locations.
Kidney Renin–Angiotensin System: Lost in a RAS Cascade
Authors: Crowley Steven D. , Navar L. Gabriel , Prieto Minolfa C. , Gurley Susan B. , Coffman Thomas M.
Published: March 2024

Renin was discovered more than a century ago. Since then, the functions of the renin–angiotensin system in the kidney have been the focus of intensive research revealing its importance in regulation of renal physiology and in the pathogenesis of heart, vascular, and kidney diseases. Inhibitors of renin–angiotensin system components are now foundational therapies for a range of kidney and cardiovascular diseases from hypertension to heart failure to diabetic nephropathy. Despite years of voluminous research, emerging studies continue to reveal new complexities of the regulation of the renin–angiotensin system within the kidney and identification of nonclassical components of the system like the prorenin receptor (PRR) and ACE2 (angiotensin-converting enzyme 2), with powerful renal effects that ultimately impact the broader cardiovascular system. With the emergence of a range of novel therapies for cardiovascular and kidney diseases, the importance of a detailed understanding of the renin–angiotensin system in the kidney will allow for the development of informed complementary approaches for combinations of treatments that will optimally promote health and longevity over the century ahead.
Tempering the Security Council’s Expanded Perception of Threats to the Peace
Authors: Klamberg Mark , Lundgren Magnus , Sundström Karin , Ahlin Per
Published: March 2024

The United Nations (UN) Security Council has expanded its understanding of threats to the peace with varying support from states. Some members of the Council have simultaneously sought to temper this development, both by providing caveats in the text of resolutions and by making statements when adopting the resolutions. This article examines how the Security Council and its members have justified their positions and actions in situations that may constitute threats to the peace. Existing scholarship has covered conceptual matters relating to Chapters vi and vii of the UN Charter, often illustrated with selected incidents. The contribution of this study is a systematic empirical analysis of how references to threats to the peace have evolved at and within the UN Security Council 1989–2019. The study combines broad quantitative analysis with qualitative case studies illustrating both the expansion of the Council’s perception of threats and the attempts by some states to temper this expansion.
Children as next of kin’s experiences, practices, and voice in everyday life: a systematic review of studies with Norwegian data (2010–2022)
Authors: Ytterhus Borgunn , Hafting Marit , Vallesverd Vibecke Ulvær , Wiig Eli Marie , Kallander Ellen Katrine , Trondsen Marianne Vibeke
Published: March 2024

Aims: This systematic review aims to identify and describe how children of parents with mental illness, substance dependence, or severe physical illness/injury, experience and practise their everyday life. Methods: The review followed the four stepwise recommendations of Harden and colleagues when including quantitative and qualitative studies on peoples’ experiences and views. In all, 23 studies with data from Norway (2010–2022) have been included. Brown and Clark’s thematic analysis was applied. Results: Three themes were constructed from the reviewed articles: (a) Children practice their relational agency by actively doing practical tasks, occasionally jobs to maintain family economy, and organising fun activities with the ill parent. (b) Emotional ambivalence when their own needs were set aside in favour of the parents. They loved their parents but also felt guilt, anger, disappointment, shame, fear of inheriting the illness and longed for a ‘normal’ everyday life. (c) Supportive contextual factors were, for example, at least one significant adult recognising them, participating in leisure activities, socialising with friends, and talking with other peers who shared similar experiences as next of kin. Obstructive factors were lack of information and recognition as well as silence and lack of dialogue within the family and/or health professional. Conclusions: There is a strong need for more knowledge and competence on the situation and needs of these children when it comes to professionals, parents and the public. Public health initiatives are needed to honour their agency and recognise their contributions in present time to prevent psychosocial problems later in life.
Bone metastasis in non-small-cell lung cancer: genomic characterization and exploration of potential targets
Authors: Gong Jiali , Hu Shumin , Shan Qianyun , Qin Jing , Han Na , Xie Fajun , Lu Hongyang
Published: March 2024

Background:

Bone metastasis (BM) seriously affects the quality of life and reduces the survival time of patients with non-small-cell lung cancer (NSCLC). The genomic characteristics and potential targets of BMs are yet to be fully explored.

Objective:

To explore the genetic characteristics and potential targets of BM in NSCLC.

Design:

In all, 83 patients with NSCLC were retrospectively selected in this study. Genomic characterization of BMs was explored with the analysis of NGS results from primary tumors and BMs in 6 patients, then combined with NGS results of lung tumors in 16 patients with initial recurrence in bone to analyze mutations potentially associated with BMs, and finally, the correlation was further validated in 61 postoperative patients.

Methods:

The next generation sequencing (NGS) was performed to identify genomic differences between pulmonary primary tumors and BM. Fluorescence in situ hybridization and immunohistochemistry were performed in postoperative tumor tissues from patients who had undergone radical surgery to validate the predictive role of molecular targets for BM. The correlation between cyclin-dependent kinase 4 ( CDK4) and BM was evaluated by Pearson’s chi-square test. The university of alabama at birminghan cancer data analysis portal (UALCAN) was carried out for the detection of CDK4 expression in lung cancer and the relationship between CDK4 and clinicopathological parameters. The relationship between prognosis and CDK4 expression was analyzed by the Kaplan–Meier plotter.

Results:

The rate of gene amplification was increased (24% versus 36%) while gene substitution/indel was decreased (64% versus 52%) in BMs. The BM-specific mutations were analyzed in 16 recurrent patients which revealed the highest incidence of CDK4 amplification (18.8%). According to the Kaplan–Meier plotter database, the NSCLC patients with high CDK4 gene expression showed poor overall survival (OS) and recurrence-free survival (RFS) ( p < 0.05). The incidence of CDK4 amplification tended to be higher in recurrent patients compared to the patients without BM (18.8% versus 4.7%, p = 0.118).

Conclusion:

Compared to the primary tumors of NSCLC, the genome of BMs showed an increased proportion of amplification and a decreased proportion of gene substitution/indel. Furthermore, the CDK4 amplification ratio seemed to be elevated in NSCLC patients with BM which may be associated with poor OS and RFS.
Force Compensation Control for Electro-Hydraulic Servo System with Pump–Valve Compound Drive via QFT–DTOC
Authors: Ba Kaixian , Wang Yuan , He Xiaolong , Wang Chunyu , Yu Bin , Liu Yaliang , Kong Xiangdong
Published: March 2024

Each joint of a hydraulic-driven legged robot adopts a highly integrated hydraulic drive unit (HDU), which features a high power–weight ratio. However, most HDUs are throttling-valve-controlled cylinder systems, which exhibit high energy losses. By contrast, pump control systems offer a high efficiency. Nevertheless, their response ability is unsatisfactory. To fully utilize the advantages of pump and valve control systems, in this study, a new type of pump–valve compound drive system (PCDS) is designed, which can not only effectively reduce the energy loss, but can also ensure the response speed and response accuracy of the HDUs in robot joints to satisfy the performance requirements of robots. Herein, considering the force control requirements of energy conservation, high precision, and fast response of the robot joint HDU, a nonlinear mathematical model of the PCDS force control system is first introduced. In addition, pressure–flow nonlinearity, friction nonlinearity, load complexity and variability, and other factors affecting the system are considered, and a novel force control method based on quantitative feedback theory (QFT) and a disturbance torque observer (DTO) is designed, which is denoted as QFT–DTOC herein. This method improves the control accuracy and robustness of the force control system, reduces the effect of the disturbance torque on the control performance of the servo motor, and improves the overall force control performance of the system. Finally, experimental verification is performed using the PCDS performance test platform. The experimental results and quantitative data show that the QFT–DTOC proposed herein can significantly improve the force control performance of the PCDS. The relevant force control method can be used as a bottom-control method for the hydraulic servo system to provide a foundation for implementing the top-level trajectory planning of the robot.
Differential cognitive and behavioral development from 6 to 24 months in autism and fragile X syndrome
Authors: Mullin Lindsay J. , Rutsohn Joshua , Gross Julia L. , Caravella Kelly E. , Grzadzinski Rebecca L. , Weisenfeld Leigh Anne , Flake Lisa , Botteron Kelly N. , Dager Stephen R. , Estes Annette M. , Pandey Juhi , Schultz Robert T. , St. John Tanya , Wolff Jason J. , Shen Mark D. , Piven Joseph , Hazlett Heather C. , Girault Jessica B.
Published: March 2024

Background

Specifying early developmental differences among neurodevelopmental disorders with distinct etiologies is critical to improving early identification and tailored intervention during the first years of life. Recent studies have uncovered important differences between infants with fragile X syndrome (FXS) and infants with familial history of autism spectrum disorder who go on to develop autism themselves (FH-ASD), including differences in brain development and behavior. Thus far, there have been no studies longitudinally investigating differential developmental skill profiles in FXS and FH-ASD infants.

Methods

The current study contrasted longitudinal trajectories of verbal (expressive and receptive language) and nonverbal (gross and fine motor, visual reception) skills in FXS and FH-ASD infants, compared to FH infants who did not develop ASD (FH-nonASD) and typically developing controls.

Results

Infants with FXS showed delays on a nonverbal composite compared to FH-ASD (as well as FH-nonASD and control) infants as early as 6 months of age. By 12 months an ordinal pattern of scores was established between groups on all domains tested, such that controls > FH-nonASD > FH-ASD > FXS. This pattern persisted through 24 months. Cognitive level differentially influenced developmental trajectories for FXS and FH-ASD.

Conclusions

Our results demonstrate detectable group differences by 6 months between FXS and FH-ASD as well as differential trajectories on each domain throughout infancy. This work further highlights an earlier onset of global cognitive delays in FXS and, conversely, a protracted period of more slowly emerging delays in FH-ASD. Divergent neural and cognitive development in infancy between FXS and FH-ASD contributes to our understanding of important distinctions in the development and behavioral phenotype of these two groups.
Evolutionary history of an Irano-Turanian cushion-forming legume (Onobrychis cornuta)
Authors: Tayebi Zahra , Moghaddam Mahtab , Mahmoodi Mohammad , Kazempour-Osaloo Shahrokh
Published: March 2024

The Irano-Turanian region is one of the largest floristic regions in the world and harbors a high percentage of endemics, including cushion-like and dwarf-shrubby taxa. Onobrychis cornuta is an important cushion-forming element of the subalpine/alpine flora of the Irano-Turanian floristic region. To specify the genetic diversity among the populations of this species (including individuals of O. elymaitica), we employed nrDNA ITS and two noncoding regions of plastid DNA (rpl32-trnL(UAG) and trnT(UGU)-trnL(UAA)). The most striking feature of O. cornuta assemblages was the unexpectedly high nucleotide diversity in both the nDNA and cpDNA dataset. In the analyses of nuclear and plastid regions, 25 ribotypes and 42 haplotypes were found among 77 and 59 accessions, respectively, from Iran, Turkey, and Afghanistan. Network analysis of the datasets demonstrated geographic differentiation within the species. Phylogenetic analyses of all dataset retrieved O. cornuta as a non-monophyletic species due to the inclusion of O. elymaitica, comprising four distinct lineages. In addition, our analyses showed cytonuclear discordance between both nuclear and plastid topologies regarding the position of some O. cornuta individuals. The underlying causes of this inconsistency remain unclear. However, we speculate that chloroplast capture, incomplete lineage sorting, and introgression were the main reasons for this event. Furthermore, molecular dating analysis indicated that O. cornuta originated in the early Pliocene (around 4.8 Mya) and started to diversify throughout the Pliocene and in particular the Pleistocene. Moreover, O. elymaitica was reduced to a subspecific rank within the species.
Association of greenspaces exposure with cardiometabolic risk factors: a systematic review and meta-analysis
Authors: Sharifi Yasaman , Sobhani Sahar , Ramezanghorbani Nahid , Payab Moloud , Ghoreshi Behnaz , Djalalinia Shirin , Nouri Ghonbalani Zahra , Ebrahimpur Mahbube , Eslami Maysa , Qorbani Mostafa
Published: March 2024

Background

Cardiometabolic conditions are major contributors to the global burden of disease. An emerging body of evidence has associated access to and surrounding public open spaces (POS) and greenspace with cardiometabolic risk factors, including obesity, body mass index (BMI), hypertension (HTN), blood glucose (BG), and lipid profiles. This systematic review aimed to synthesize this evidence.

Methods

This systematic review was conducted based on the PRISMA guidelines. Four electronic databases including Web of Science, PubMed, Scopus, and Google Scholar were searched for eligible articles published until July 2023. All observational studies which assessed the association of greenspace and POS with cardiometabolic risk factors including obesity, BMI, HTN, BG, and lipid profiles were included and reviewed by two authors independently. Heterogeneity between studies was assessed using the I2 index and Cochrane’s Q test. Random/fixed effect meta-analyses were used to combine the association between greenspace exposure with cardiometabolic risk factors.

Results

Overall, 118 relevant articles were included in our review. The majority of the articles were conducted in North America or Europe. In qualitative synthesis, access or proximity to greenspaces or POS impacts BMI and blood pressure or HTN, BG, and lipid profiles via various mechanisms. According to the random effect meta-analysis, more access to greenspace was significantly associated with lower odds of HTN (odds ratio (OR): 0.81, 95% confidence intervals (CIs): 0.61–0.99), obesity (OR: 0.83, 95% CIs: 0.77–0.90), and diabetes (OR:0.79, 95% CI: 0.67,0.90).

Conclusions

Findings of this systematic review and meta-analysis suggested that greenspace accessibility is associated with some cardiometabolic risk factors. Improving greenspace accessibility could be considered as one of the main strategies to reduce cardiometabolic risk factors at population level.
Characterization of primary small intestinal lymphoma: a retrospective study based on double balloon endoscopy
Authors: Li Lin , Ma Huijian , Niu Meng , Chen Chunxiao , Yu Chaohui , Zhang Hong , Jin Meng
Published: March 2024

Background

The diagnosis of primary small intestinal lymphoma (PSIL) is difficult. This study aimed to evaluate the clinical, radiological and endoscopic characteristics of PSIL and provide clue for diagnosis.

Methods

A total of 30 patients diagnosed with PSIL who underwent double balloon endoscopy (DBE) in the First Affiliated Hospital of Zhejiang University were retrospectively analyzed. Clinical, radiological and endoscopic data were collected. Univariate analysis was used to determine significant indicators for differentiating three main subtypes of PSIL. Cox regression analysis was performed to assess the risk factors for survival.

Results

In this study, 10 patients were pathologically diagnosed as diffuse large B-cell lymphoma (DLBCL), 11 were indolent B-cell lymphoma (BCL) and 9 were T-cell lymphoma (TCL). Compared with DLBCL patients, the body mass index (BMI) of TCL patients was significantly lower (p = 0.004). Meanwhile, compared with patients with DLBCL, the patients with indolent BCL had lower levels of C-reactive protein, lactate dehydrogenase (LDH), fibrinogen and D-Dimer (p = 0.004, p = 0.004, p = 0.006, and p = 0.002, respectively), and lower proportion of thicker intestinal wall and aneurysmal dilation in CT scan (p = 0.003 and p = 0.020, respectively). In terms of ulcer morphology, patients with DLBCL had significantly higher proportion of deep ulcers than patients with indolent BCL (p = 0.020, respectively). Cox regression analysis showed that drink (p = 0.034), concomitant colonic ulcers (p = 0.034) and elevated LDH (p = 0.043) are risk factors for mortality in patients with PSIL.

Conclusions

This study provides clinical characteristics of patients with PSIL. Thicker intestinal wall and aneurismal dilation detected on CT scan and deeper ulcer on DBE examination helps to establish a diagnosis of DLBCL.
Possible potential spread of Anopheles stephensi, the Asian malaria vector
Authors: Liu Qing , Wang Ming , Du Yu-Tong , Xie Jing-Wen , Yin Zi-Ge , Cai Jing-Hong , Zhao Tong-Yan , Zhang Heng-Duan
Published: March 2024

Background

Anopheles stephensiis native to Southeast Asia and the Arabian Peninsula and has emerged as an effective and invasive malaria vector. Since invasion was reported in Djibouti in 2012, the global invasion range ofAn. stephensihas been expanding, and its high adaptability to the environment and the ongoing development of drug resistance have created new challenges for malaria control. Climate change is an important factor affecting the distribution and transfer of species, and understanding the distribution ofAn. stephensiis an important part of malaria control measures, including vector control.

Methods

In this study, we collected existing distribution data forAn. stephensi, and based on the SSP1-2.6 future climate data, we used the Biomod2 package in R Studio through the use of multiple different model methods such as maximum entropy models (MAXENT) and random forest (RF) in this study to map the predicted globalAn. stephensiclimatically suitable areas.

Results

According to the predictions of this study, some areas where there are no current records ofAn. stephensi, showed significant areas of climatically suitable forAn. stephensi. In addition, the global climatically suitability areas forAn. stephensiare expanding with global climate change, with some areas changing from unsuitable to suitable, suggesting a greater risk of invasion ofAn. stephensiin these areas, with the attendant possibility of a resurgence of malaria, as has been the case in Djibouti.

Conclusions

This study provides evidence for the possible invasion and expansion ofAn. stephensiand serves as a reference for the optimization of targeted monitoring and control strategies for this malaria vector in potential invasion risk areas.
Genome-wide association study of nausea and vomiting during pregnancy in Japan: the TMM BirThree Cohort Study
Authors: Yonezawa Yudai , Takahashi Ippei , Ohseto Hisashi , Ueno Fumihiko , Onuma Tomomi , Noda Aoi , Murakami Keiko , Ishikuro Mami , Obara Taku , Kuriyama Shinichi
Published: March 2024

Background

Nausea and vomiting during pregnancy (NVP) and hyperemesis gravidarum (HG), common conditions affecting most pregnant women, are highly heritable and associated with maternal and fetal morbidity. However, the pathologies underlying NVP and HG and their associated loci are scarce.

Methods

We performed genome-wide association studies (GWAS) of NVP in pregnant women (n = 23,040) who participated in the Tohoku Medical Megabank Project Birth and Three-Generation Cohort Study in Japan from July 2013 to March 2017. Participants were divided into discovery (n = 9,464) and replication (n = 10,051) stages based on the platform used for their genotyping. Loci that achieved the genome-wide significance level (p < 5.0 × 10− 8) in the discovery stage were selected for genotyping in the replication stage. A meta-analysis integrating the discovery and replication stage results (n = 19,515) was conducted. NVP-related variables were identified as categorical or continuous.

Results

GWAS analysis in the discovery phase revealed loci linked to NVP in two gene regions, 11q22.1 (rs77775955) and 19p13.11 (rs749451 and rs28568614). Loci in these two gene regions have also been shown to be associated with HG in a White European population, indicating the generalizability of the GWAS analyses conducted in this study. Of these, only rs749451 and rs28568614 at 19p13.11 reached the genome-wide suggestive level (p < 1.0 × 10− 5) in the replication stage; however, both loci were significant in the meta-analysis.

Conclusions

NVP-related loci were identified in the Japanese population at 11q22.1 and 19p13.11, as reported in previous GWAS. This study contributes new evidence on the generalizability of previous GWAS on the association between genetic background and NVP.
Induced erotomania by online romance fraud - a novel form of de Clérambault’s syndrome
Authors: Alotti Nasri , Osvath Peter , Tenyi Tamas , Voros Viktor
Published: March 2024

Background

Although the impact of internet usage on mental health is extensively documented, there is a notable scarcity of reports in the literature concerning internet-induced erotomania. Erotomania is a rare and likely underdiagnosed delusional disorder. It is characterized by an irrational belief held by the affected persons that someone of higher socioeconomic status harbor romantic feelings toward them. Here, we describe the psychopathology of erotomanic delusion induced by online romantic fraud in a female patient. Employing this case as a focal point, we illuminate novel aspects of erotomania that warrant attention and examination.

Case presentation

We present a compelling case involving a 70-year-old married Caucasian woman diagnosed with medically controlled persistent depressive disorder for several years. The intricacies of her condition became evident as she became deeply engrossed in online profiles featuring the image of a renowned musician, inadvertently falling victim to an online romantic fraud. Subsequently, this distressing experience triggered the emergence of erotomanic delusions and a suicide attempt. The patient's history reveals an array of medical conditions and stressful life events, contributing to her vulnerability. The diagnosis of erotomanic delusional disorder, dysthymia, and mild cognitive impairment with cerebral vascular background was established. Treatment involved her previous antidepressant with low-dose risperidone, alongside supportive individual and group therapy. Her delusion showed remission four weeks later, prompting her discharge for outpatient follow-up. Although she retained some false beliefs, the intensity of the symptoms had notably diminished and her functionality improved.

Conclusion

This case underscores the complex interplay between mental health, online activities, and the consequences of delusions, including suicidal thoughts, shedding light on the need for a comprehensive approach in addressing such challenging psychiatric scenarios.
How individuals’ opinions influence society’s resistance to epidemics: an agent-based model approach
Authors: Yu Geonsik , Garee Michael , Ventresca Mario , Yih Yuehwern
Published: March 2024

Background

Protecting public health from infectious diseases often relies on the cooperation of citizens, especially when self-care interventions are the only viable tools for disease mitigation. Accordingly, social aspects related to public opinion have been studied in the context of the recent COVID-19 pandemic. However, a comprehensive understanding of the effects of opinion-related factors on disease spread still requires further exploration.

Methods

We propose an agent-based simulation framework incorporating opinion dynamics within an epidemic model based on the assumption that mass media channels play a leading role in opinion dynamics. The model simulates how opinions about preventive interventions change over time and how these changes affect the cumulative number of cases. We calibrated our simulation model using YouGov survey data and WHO COVID-19 new cases data from 15 different countries. Based on the calibrated models, we examine how different opinion-related factors change the consequences of the epidemic. We track the number of total new infections for analysis.

Results

Our results reveal that the initial level of public opinion on preventive interventions has the greatest impact on the cumulative number of cases. Its normalized permutation importance varies between 69.67% and 96.65% in 15 models. The patterns shown in the partial dependence plots indicate that other factors, such as the usage of the pro-intervention channel and the response time of media channels, can also bring about substantial changes in disease dynamics, but only within specific ranges of the dominant factor.

Conclusions

Our results reveal the importance of public opinion on intervention during the early stage of the pandemic in protecting public health. The findings suggest that persuading the public to take actions they may be hesitant about in the early stages of epidemics is very costly because taking early action is critical for mitigating infectious diseases. Other opinion-related factors can also lead to significant changes in epidemics, depending on the average level of public opinion in the initial stage. These findings underscore the importance of media channels and authorities in delivering accurate information and persuading community members to cooperate with public health policies.
Integrating palliative care education in pulmonary rehabilitation: a randomized controlled study protocol
Authors: Mendes M. Aurora , Janssen Daisy J. A. , Marques Alda
Published: March 2024

Background

Palliative care addresses multiple unmet needs of people with chronic obstructive pulmonary disease (COPD) or interstitial lung diseases (ILD) and their family and/or friend caregivers, but it remains highly underused. Pulmonary rehabilitation (PR) may provide a key opportunity to introduce palliative care. We aim to explore the effects of palliative care education as part of PR on knowledge about this field in people with COPD or ILD and their family and/or friend caregivers.

Methods

A randomized controlled study will compare PR with palliative care education (experimental) with traditional PR (control) in people with COPD or ILD and their family and/or friend caregivers. Family and/or friend caregivers will be invited to take part in education and psychosocial support sessions. In addition to the usual educational content, the experimental group will have a session on palliative care, a “Peer-to-peer session”, two “Get-apart sessions” and online sessions. The “Peer-to-peer session” and the “Get-apart sessions” will be discussions about topics suggested by participants. The “Get-apart sessions” will be dedicated to people with COPD or ILD apart from their family and/or friend caregivers and vice versa. The online sessions will be zoom meetings to discuss any health-related issues raised by participants, at a flexible time. A mixed-methods approach will be used to evaluate the outcomes. The primary outcome will be knowledge about palliative care. Secondary outcomes will include attitude towards palliative care referral, symptoms, disease impact, health-related quality of life, needs, knowledge about the disease, burden of providing care, adherence, adverse events and referral to a specialist palliative care team. Quantitative and qualitative data will be collected at baseline and end of PR. At 6-months post-PR, only patient-reported outcomes will be collected. For the primary outcome, time*group interaction will be analyzed with mixed analysis of variance.

Discussion

This study aims to demonstrate the impact of integrating palliative care into the PR education program.

Trial registration

The trial was registered in the ClinicalTrials.gov U.S. National Library of Medicine, on 1st September, 2023 (NCT06046547).
Deletion of Slc9a1 in Cx3cr1+ cells stimulated microglial subcluster CREB1 signaling and microglia-oligodendrocyte crosstalk
Authors: Song Shanshan , Oft Helena , Metwally Shamseldin , Paruchuri Satya , Bielanin John , Fiesler Victoria , Sneiderman Chaim , Kohanbash Gary , Sun Dandan
Published: March 2024

Microglial Na/H exchanger-1 (NHE1) protein, encoded by Slc9a1, plays a role in white matter demyelination of ischemic stroke brains. To explore underlying mechanisms, we conducted single cell RNA-seq transcriptome analysis in conditional Slc9a1 knockout (cKO) and wild-type (WT) mouse white matter tissues at 3 days post-stroke. Compared to WT, Nhe1 cKO brains expanded a microglial subgroup with elevated transcription of white matter myelination genes including Spp1, Lgals3, Gpnmb, and Fabp5. This subgroup also exhibited more acidic pHi and significantly upregulated CREB signaling detected by ingenuity pathway analysis and flow cytometry. Moreover, the Nhe1 cKO white matter tissues showed enrichment of a corresponding oligodendrocyte subgroup, with pro-phagocytosis and lactate shuffling gene expression, where activated CREB signaling is a likely upstream regulator. These findings demonstrate that attenuation of NHE1-mediated H+ extrusion acidifies microglia/macrophage and may underlie the stimulation of CREB1 signaling, giving rise to restorative microglia-oligodendrocyte interactions for remyelination.
Incorporation of the histone variant H2A.Z counteracts gene silencing mediated by H3K27 trimethylation in Fusarium fujikuroi
Authors: Atanasoff-Kardjalieff Anna K. , Berger Harald , Steinert Katharina , Janevska Slavica , Ponts Nadia , Humpf Hans-Ulrich , Kalinina Svetlana , Studt-Reinhold Lena
Published: March 2024

Background

Fusarium fujikuroi is a pathogen of rice causing diverse disease symptoms such as ‘bakanae’ or stunting, most likely due to the production of various natural products (NPs) during infection. Fusaria have the genetic potential to synthesize a plethora of these compounds with often diverse bioactivity. The capability to synthesize NPs exceeds the number of those being produced by far, implying a gene regulatory network decisive to induce production. One such regulatory layer is the chromatin structure and chromatin-based modifications associated with it. One prominent example is the exchange of histones against histone variants such as the H2A variant H2A.Z. Though H2A.Z already is well studied in several model organisms, its regulatory functions are not well understood. Here, we used F. fujikuroi as a model to explore the role of the prominent histone variant FfH2A.Z in gene expression within euchromatin and facultative heterochromatin.

Results

Through the combination of diverse ‘-omics‘ methods, we show the global distribution of FfH2A.Z and analyze putative crosstalks between the histone variant and two prominent histone marks, i.e., H3K4me3 and H3K27me3, important for active gene transcription and silencing, respectively. We demonstrate that, if FfH2A.Z is positioned at the + 1-nucleosome, it poises chromatin for gene transcription, also within facultative heterochromatin. Lastly, functional characterization of FfH2A.Z overexpression and depletion mutants revealed that FfH2A.Z is important for wild type-like fungal development and secondary metabolism.

Conclusion

In this study, we show that the histone variant FfH2A.Z is a mark of positive gene transcription and acts independently of the chromatin state most likely through the stabilization of the + 1-nucleosome. Furthermore, we demonstrate that FfH2A.Z depletion does not influence the establishment of both H3K27me3 and H3K4me3, thus indicating no crosstalk between FfH2A.Z and both histone marks. These results highlight the manifold functions of the histone variant FfH2A.Z in the phytopathogen F. fujikuroi, which are distinct regarding gene transcription and crosstalk with the two prominent histone marks H3K27me3 and H3K4me3, as proposed for other model organisms.
Patients with systemic sclerosis and low CD4 numbers after autologous stem cell transplantation have a favorable outcome
Authors: Pecher Ann-Christin , Klein Reinhild , Koetter Ina , Wagner Marieke , Vogel Wichard , Wirths Stefan , Lengerke Claudia , Henes Joerg Christoph
Published: March 2024

Background

Treatment with high-dose chemotherapy followed by autologous hematopoietic stem cell transplantation (aHSCT) is an intensive treatment option for patients with severe forms of systemic sclerosis (SSc). Even though associated with a high treatment related mortality, the results in this high-risk population are generally favourable. The knowledge on the potential mechanism of action of this therapy and how it can improve patients with SSc is crucial to better select the right patients for aHSCT.

Methods

This is a monocentric retrospective study from Tübingen, Germany, including 32 patients who underwent aHSCT. Peripheral blood samples were analysed for different lymphocyte subsets at various timepoints before and after aHSCT. Patients were divided into responders and non-responders according to the modified Rodnan skin score and lung function test in the three years following aHSCT.

Results

Responders showed significantly lower levels of cluster of differentiation (CD)4 positive T cells in the first months after aHSCT (month 1 and 3), B cells (month 3 and 6 after aHSCT) and natural killer cells (month 1). Mantel-cox test showed a significant deviation of the probability curves, i.e. patients with lower CD4 + T cells and natural killer cells one month and B cells after 3 months after stem cell transplantation had a higher probability to belong to the responder group.

Conclusions

Taken together, this study supports the theory that a profound CD4 + T cell and B cell lymphopenia is important for patients with SSc to achieve a sustained response after aHSCT.
Long-term outcome of combined radiologic and surgical strategy for the management of biliary complications after pediatric liver transplantation
Authors: Calinescu Ana M. , Monluc Sébastien , Franchi-Abella Stephanie , Habes Dalila , Weber Gabrielle , Almes Marion F. , Waguet Jerome , Jacquemin Emmanuel , Fouquet Virginie , Miatello Jordi , Hery Geraldine , Baujard Catherine , Gonzales Emmanuel , Branchereau Sophie , Guérin Florent
Published: March 2024

Objectives

We aimed to analyze the risk factors for management failure of BC after pediatric liver transplantation (pLT) by retrospectively analyzing primary pLT performed between 1997 and 2018 (n = 620 patients).

Results

In all, 117/620 patients (19%) developed BC. The median (range) follow-up was 9 (1.4–21) years. Patient survival at 1, 5 and 10 years was 88.9%, 85.7%, 84.4% and liver graft survival was 82.4%, 77.4%, and 74.3% respectively. Graft not patient survival was impaired by BC (p = 0.01). Multivariate analysis identified the number of dilatation courses > 2 (p = 0.008), prolonged cold ischemia time (p = 0.004), anastomosed multiple biliary ducts (p = 0.019) and hepatic artery thrombosis (p = 0.01) as factors associated with impaired graft survival. The number of dilatation courses > 2 (p < 0.001) and intrahepatic vs anastomotic stricture (p = 0.014) were associated with management failure. Thus, repeated (> 2) radiologic dilatation courses are associated with impaired graft survival and management failure. Overall, graft but not patient survival was impaired by BC.
Fulminant coronavirus disease 2019 meningitis in Iranian infants: a case series
Authors: Afsharpayman Shahla , Madani Sedigheh , Amirsalari Susan , Momeni Nooradin , Torkaman Mohammad , Beiraghdar Fatemeh , Kavehmanesh Zohreh , Hosseininezhad Zahra
Published: March 2024

Background

Pediatric coronavirus disease 2019 infection usually presents with respiratory and gastrointestinal symptoms. In this report we present fulminant meningitis as the main presentation of coronavirus disease 2019 without major signs and symptoms of other organs’ involvement in 3 infants.

Cases

The first case was a 4 months Iranian male infant with fulminant meningitis as the main presentation of coronavirus disease 2019 without other organ involvement. He was treated as suspected bacterial meningitis but CSF PCR and CSF culture were negative for common meningeal pathogens. On 3rd day, his coronavirus disease 2019 PCR test became positive, while it was negative on 1st day. The second case was a 13 months Iranian male infant with fever, irritability, and photophobia for 24 h before poorly controlled status epilepticus. CSF coronavirus disease 2019 PCR became positive while CSF PCR and CSF culture were negative for other common meningeal pathogens. Seizures were controlled with multiple anti-seizure medications. The third case was a 14 months Iranian female infant with fever and seizure1 hour before admission, leading to poorly controlled status epilepticus despite anti-epileptic therapy 10 h after admission. CSF coronavirus disease 2019 PCR became positive while CSF PCR and CSF culture were negative for other common meningeal pathogens. He was controlled with multiple anti-seizure medications.

Conclusion

Meningitis of coronavirus disease 2019 should be considered in severely ill pediatric cases with poorly controlled seizures and RBC in CSF smear. Also, pediatricians can consider corticosteroids, remdesivir, and IVIG therapy in these cases.
Systematic review of the molecular basis of hereditary breast and ovarian cancer syndrome in Brazil: the current scenario
Authors: de Freitas Ribeiro Andreza Amália , Junior Nilson Moreira Cipriano , dos Santos Luciana Lara
Published: March 2024

Background

A detailed understanding of the genetic basis of cancer is of great interest to public health monitoring programs. Although many studies have been conducted in Brazil, a global view on the molecular profile related to hereditary breast and ovarian cancer (HBOC) in this large and heterogeneous population is lacking.

Methods

A systematic review following the PRISMA guidelines was conducted in three electronic databases (PubMed, BIREME and SciELO). Brazilian studies covering molecular analysis of genes related to HBOC, published until December 2023, were considered.

Results

We identified 35 original studies that met all the inclusion criteria. A total of 137 distinct mutations were found in the BRCA1 gene, but four of them corresponded to 44.5% of all mutations found in this gene. The c.5266dupC BRCA1 mutation was responsible for 26.8% of all pathogenic mutations found in the BRCA1 gene in patients with clinical criteria for HBOC from the Brazilian population. Considering all studies that track this mutation in the BRCA1 gene, we found a frequency of 2% (120/6008) for this mutation in Brazilian patients. In the BRCA2 gene, the four most frequent mutations corresponded to 29.2% of pathogenic mutations. Even though it was tracked by few studies, the c.156_157insAlu mutation was responsible for 9.6% of all pathogenic mutations reported in the BRCA2 gene. Seventeen studies found pathogenic mutations in other non-BRCA genes, the c.1010G > A mutation in the TP53 gene being the most frequent one. Considering all studies that screened for this specific mutation in patients with the clinical criteria for HBOC, the frequency of c.1010G > A was estimated at 1.83% (61/3336).

Conclusions

Despite significant molecular heterogeneity among mutations in HBOC patients from Brazil, three mutations deserve to be highlighted, c.5266dupC, c.156_157insAlu and c.1010G > A in the BRCA1, BRCA2 and TP53 genes, respectively. With more than 200 records, these three mutations play a vital role in the pathology of breast and ovarian cancer in Brazil. The data collected shed light on the subject, but there is still not enough data from certain subpopulations.
Interface Shear Failure Behavior Between Normal Concrete (NC) and Ultra-High Performance Concrete (UHPC)
Authors: Zhang Boshan , Yu Jiangjiang , Chen Weizhen , Chen Jianbo , Li Heng , Niu Jialun
Published: March 2024

Ultra-high performance concrete (UHPC) with excellent mechanical properties and durability is a promising material for reinforcement of existing normal concrete (NC) structures. In this paper, the shear failure behavior of the NC–UHPC interface was studied by the slant shear test and the SEM (scanning electron microscope) visualization test, considering influence of the substrate strength and the interface roughed treatment. As the NC substrate and the UHPC overlay are tightly combined at the interface transition zone (ITZ), the interface exhibits good slant shear performance, and the measured interfacial shear strength could reach 19.4 MPa with C40 substrate and 21.8 MPa with C50 substrate. In addition, the microstructure and composition of the ITZ, the possible interfacial failure modes, and the load-carrying mechanism of the interface under compression–shear force are revealed. The high interface roughness and the substrate strength have positive influence on the shear strength, and greatly affect the prone failure mode and the load-slip characteristic.
Updated Analysis of Comparative Toxicity of Proton and Photon Radiation for Prostate Cancer
Authors: Yu James B. , DeStephano David M. , Jeffers Brian , Horowitz David P. , Soulos Pamela R. , Gross Cary P. , Cheng Simon K.
Published: March 2024

PURPOSE

Previous comparative effectiveness studies have not demonstrated a benefit of proton beam therapy (PBT) compared with intensity-modulated radiation therapy (IMRT) for prostate cancer. An updated comparison of GI and genitourinary (GU) toxicity is needed.

METHODS

We investigated the SEER-Medicare linked database, identifying patients with localized prostate cancer diagnosed from 2010 to 2017. Procedure and diagnosis codes indicative of treatment-related toxicity were identified. As a sensitivity analysis, we also identified toxicity based only on procedure codes. Patients who underwent IMRT and PBT were matched 2:1 on the basis of clinical and sociodemographic characteristics. We then compared GI and GU toxicity at 6, 12, and 24 months after treatment.

RESULTS

The final sample included 772 PBT patients matched to 1,544 IMRT patients. The frequency of GI toxicity for IMRT versus PBT was 3.5% versus 2.5% at 6 months ( P = .18), 9.5% versus 10.2% at 12 months ( P = .18), and 20.5% versus 23.4% at 24 months ( P = .11). The frequency of only procedure codes indicative of GI toxicity for IMRT versus PBT was too low to be reported and not significantly different. The frequency of GU toxicity for IMRT versus PBT was 6.8% versus 5.7% ( P = .30), 14.3% versus 12.2% ( P = .13), and 28.2% versus 25.8% ( P = .21) at 6, 12, and 24 months, respectively. When looking only at procedure codes, the frequency of GU toxicity for IMRT was 1.0% at 6 months, whereas it was too infrequent to report for PBT ( P = .64). GU toxicity for IMRT versus PBT was 3.3% versus 2.1% ( P = .10), and 8.7% versus 6.7% ( P = .10) at 12 and 24 months, respectively.

CONCLUSION

In this observational study, there were no statistically significant differences between PBT and IMRT in terms of GI or GU toxicity.