bluuebunny/crossref_metadata_2025 huggingface dataset:
Hallmarks for Thrombotic and Hemorrhagic Risks in Chronic Kidney Disease Patients
Authors: Saeed Zeeba , Sirolli Vittorio , Bonomini Mario , Gallina Sabina , Renda Giulia
Published: August 2024

Chronic kidney disease (CKD) is a global health issue causing a significant health burden. CKD patients develop thrombotic and hemorrhagic complications, and cardiovascular diseases are associated with increased hospitalization and mortality in this population. The hemostatic alterations are multifactorial in these patients; therefore, the results of different studies are varying and controversial. Endothelial and platelet dysfunction, coagulation abnormalities, comorbidities, and hemoincompatibility of the dialysis membranes are major contributors of hypo- and hypercoagulability in CKD patients. Due to the tendency of CKD patients to exhibit a prothrombotic state and bleeding risk, they require personalized clinical assessment to understand the impact of antithrombotic therapy. The evidence of efficacy and safety of antiplatelet and anticoagulant treatments is limited for end-stage renal disease patients due to their exclusion from major randomized clinical trials. Moreover, designing hemocompatible dialyzer membranes could be a suitable approach to reduce platelet activation, coagulopathy, and thrombus formation. This review discusses the molecular mechanisms underlying thrombotic and hemorrhagic risk in patients with CKD, leading to cardiovascular complications in these patients, as well as the evidence and guidance for promising approaches to optimal therapeutic management.
Molecular Determinants for Photodynamic Therapy Resistance and Improved Photosensitizer Delivery in Glioma
Authors: Aebisher David , Woźnicki Paweł , Czarnecka-Czapczyńska Magdalena , Dynarowicz Klaudia , Szliszka Ewelina , Kawczyk-Krupka Aleksandra , Bartusik-Aebisher Dorota
Published: August 2024

Gliomas account for 24% of all the primary brain and Central Nervous System (CNS) tumors. These tumors are diverse in cellular origin, genetic profile, and morphology but collectively have one of the most dismal prognoses of all cancers. Work is constantly underway to discover a new effective form of glioma therapy. Photodynamic therapy (PDT) may be one of them. It involves the local or systemic application of a photosensitive compound—a photosensitizer (PS)—which accumulates in the affected tissues. Photosensitizer molecules absorb light of the appropriate wavelength, initiating the activation processes leading to the formation of reactive oxygen species and the selective destruction of inappropriate cells. Research focusing on the effective use of PDT in glioma therapy is already underway with promising results. In our work, we provide detailed insights into the molecular changes in glioma after photodynamic therapy. We describe a number of molecules that may contribute to the resistance of glioma cells to PDT, such as the adenosine triphosphate (ATP)-binding cassette efflux transporter G2, glutathione, ferrochelatase, heme oxygenase, and hypoxia-inducible factor 1. We identify molecular targets that can be used to improve the photosensitizer delivery to glioma cells, such as the epithelial growth factor receptor, neuropilin-1, low-density lipoprotein receptor, and neuropeptide Y receptors. We note that PDT can increase the expression of some molecules that reduce the effectiveness of therapy, such as Vascular endothelial growth factor (VEGF), glutamate, and nitric oxide. However, the scientific literature lacks clear data on the effects of PDT on many of the molecules described, and the available reports are often contradictory. In our work, we highlight the gaps in this knowledge and point to directions for further research that may enhance the efficacy of PDT in the treatment of glioma.
Targeting Peptidylarginine Deiminase 3 to Efficiently Suppress Herpes Simplex Virus Type 2 Infection
Authors: Pasquero Selina , Gugliesi Francesca , Biolatti Matteo , Albano Camilla , Bajetto Greta , Trifirò Linda , Raviola Stefano , Dell’Oste Valentina , De Andrea Marco
Published: August 2024

Protein expression is regulated through multiple mechanisms, including post-translational modifications (PTMs), which can alter protein structure, stability, localization, and function. Among these, citrullination stands out due to its ability to convert arginine residues into citrulline, altering protein charge and mass. This modification is catalyzed by calcium-dependent protein arginine deiminases (PADs), enzymes implicated in various inflammatory diseases. We have recently shown that human cytomegalovirus (HCMV) and herpes simplex virus type 1 (HSV-1) exploit these enzymes to enhance their replication capabilities. Although the role of PADs in HCMV and HSV-1 infections is well documented, their involvement in HSV-2 infection has not yet been thoroughly investigated. Here, we demonstrate that HSV-2 manipulates the overall protein citrullination profile by activating three PAD isoforms: PAD2, PAD3, and PAD4. However, as previously observed during HSV-1 infection, PAD3 is the most significantly upregulated isoform, both at the mRNA and protein levels. Consistently, we demonstrate that inhibiting PAD3, either through the specific inhibitor CAY10727 or via CRISPR/Cas9-mediated gene silencing, markedly reduces HSV-2 replication and viral protein expression. Lastly, we show that CAY10727 displays an IC50 value of 0.3 μM, which is extremely close to what was previously observed for HSV-1. Overall, our findings highlight the crucial role of PAD3 in the life cycle of HSV-2 and suggest that the targeted inhibition of PAD3 may represent a promising approach for treating HSV-2 infections, especially in cases resistant to existing antiviral therapies.
Astaxanthin, Compared to Other Carotenoids, Increases the Efficacy of Methotrexate in Rat Adjuvant Arthritis
Authors: Pružinská Katarína , Chrastina Martin , Khademnematolahi Sasan , Vyletelová Veronika , Gajdošová Lívia , Pastvová Lucia , Dráfi František , Poništ Silvester , Pašková Ľudmila , Kucharská Jarmila , Sumbalová Zuzana , Muchová Jana , Martiniaková Silvia , Bauerová Katarína
Published: August 2024

This in vivo study performed in rat adjuvant arthritis aims to advance the understanding of astaxanthin’s therapeutic properties for the possible treatment of rheumatoid arthritis (RA) in monotherapy and along with the standard RA treatment, methotrexate (MTX), in combination therapy. The main goal was to elucidate astaxanthin’s full therapeutic potential, evaluate its dose dependency, and compare its effects in monotherapy with other carotenoids such as β-carotene and β-cryptoxanthin (KXAN). Moreover, potential differences in therapeutic activity caused by using different sources of astaxanthin, synthetic (ASYN) versus isolated from Blakeslea trispora (ASTAP), were evaluated using one-way ANOVA (Tukey-Kramer post hoc test). KXAN was the most effective in reducing plasma MMP-9 levels in monotherapy, significantly better than MTX, and in reducing hind paw swelling. The differences in the action of ASTAP and ASYN have been observed across various biometric, anti-inflammatory, and antioxidative parameters. In combined therapy with MTX, the ASYN + MTX combination proved to be better. These findings, especially the significant anti-arthritic effect of KXAN and ASYN + MTX, could be the basis for further preclinical studies.
Alteration of Photosynthetic and Antioxidant Gene Expression in Sugarcane Infected by Multiple Mosaic Viruses
Authors: Neliana Intan Ria , Soleha Wardatus , Suherman  , Darsono Nurmalasari , Harmoko Rikno , Sawitri Widhi Dyah , Sugiharto Bambang
Published: August 2024

Sugarcane mosaic virus (SCMV), sugarcane streak mosaic virus (SCSMV), and sorghum mosaic virus (SrMV) are the causative pathogens of mosaic disease. This study aimed to identify mosaic virus infection and its impact on photosynthetic and antioxidant gene expression in eight commercial sugarcane cultivars grown on sugarcane plantations in East Java, Indonesia. The disease incidence and severity were observed in symptomatic leave samples, and then the virus was identified. A high incidence and severity of mosaic symptoms were observed in the PS881 and NX04 cultivars compared with the other cultivars. RT-PCR analysis detected SCSMV infection in all cultivars; double infections with SCSMV and SCMV in the PS881, PS882, and Cening cultivars; and triple infections with SCSMV, SCMV, and SrMV in the PS881 cultivar. Ascorbate peroxidase (Apx) expression was upregulated in all virus-infected cultivars and significantly increased in the triple-infected PS881 cultivar. However, catalase (Cat) expression was only slightly increased in the PS881 cultivar. The chlorophyll content was reduced, and the PsaA gene was downregulated in all cultivars. The expression of PsaA, RbcS, and Sps was significantly suppressed in the triple-infected PS881 cultivar. Moreover, the downregulation of both the RbcS and Pepc genes was concomitant with that of their protein levels.
Deep Learning-Based Monocular Estimation of Distance and Height for Edge Devices
Authors: Gąsienica-Józkowy Jan , Cyganek Bogusław , Knapik Mateusz , Głogowski Szymon , Przebinda Łukasz
Published: August 2024

Accurately estimating the absolute distance and height of objects in open areas is quite challenging, especially when based solely on single images. In this paper, we tackle these issues and propose a new method that blends traditional computer vision techniques with advanced neural network-based solutions. Our approach combines object detection and segmentation, monocular depth estimation, and homography-based mapping to provide precise and efficient measurements of absolute height and distance. This solution is implemented on an edge device, allowing for real-time data processing using both visual and thermal data sources. Experimental tests on a height estimation dataset we created show an accuracy of 98.86%, confirming the effectiveness of our method.
Safety and Effectiveness of Thiopurines and Small Molecules in Elderly Patients with Inflammatory Bowel Diseases
Authors: Strigáč Aleksandra , Caban Miłosz , Małecka-Wojciesko Ewa , Talar-Wojnarowska Renata
Published: August 2024

The management of inflammatory bowel diseases (IBD) requires weighing an individual patient’s therapeutic benefits and therapy-related complication risks. The immunomodulators that have been commonly used so far in IBD therapy are thiopurines, including 6-mercaptopurine and azathioprine. As our understanding of the IBD pathomechanisms is widening, new therapeutic approaches are being introduced, including the Janus kinase (JAK) inhibitors and Sphingosine 1-phosphate receptor (S1PR) modulators’ development. Non-selective JAK inhibitors are represented by tofacitinib, while selective JAK inhibitors comprise filgotinib and upadacitinib. As for the S1PR modulators, ozanimod and etrasimod are approved for UC therapy. The number of elderly patients with IBD is growing; therefore, this review aimed to evaluate the effectiveness and safety of the oral immunomodulators among the subjects aged ≥60. Possible complications limit the use of thiopurines in senior patients. Likewise, the promising effectiveness of new drugs in IBD therapy in those with additional risk factors might be confined by the risk of serious adverse events. However, the data regarding this issue are limited.
Preliminary Data on SNP of Transplantation-Related Genes after Haploidentical Stem Cell Transplantation
Authors: Tseng Ching-Ping , Lin Tung-Liang , Tsai Shu-Hui , Lin Wei-Tzu , Hsu Fang-Ping , Wang Wei-Ting , Chen Ding-Ping
Published: August 2024

Background: Hematopoietic stem cell transplantation (HSCT) is one of the mainstream treatments for patients with hematologic malignancies. The matching status of human leukocyte antigen (HLA) between the donor and recipient is highly related to the outcomes of HSCT. Haploidentical HSCT (haplo-HSCT) has emerged as a type of HSCT for patients who cannot find a fully HLA-matched donor. In this study, we investigated whether the single nucleotide polymorphisms (SNPs) of the HLA-related genes and the genes encoding co-stimulatory molecules located on the non-HLA region are related to the outcomes of haplo-HSCT. Methods: The genomic DNAs of 24 patients and their respective donors were isolated from the peripheral blood obtained before performing haplo-HSCT. A total of 75 SNPs of the HLA-related genes (HCP5, NOTCH4, HLA-DOA, LTA, HSPA1L, BAG6, RING1, TRIM27, and HLA-DOB) and the genes located in the non-HLA genes involved in co-stimulatory signaling (CTLA4, TNFSF4, CD28, and PDCD1) were selected to explore their relationship with the outcomes after haplo-HSCT, including graft-versus-host disease, survival status, and relapse. Results: Our data revealed that specific donor or patient SNPs, including rs79327197 of the HLA-DOA gene, rs107822 and rs213210 of the RING1 gene, rs2523676 of the HCP5 gene, rs5742909 of the CTLA4 gene, rs5839828 and rs36084323 of the PDCD1 gene, and rs1234314 of the TNFSF4 gene, were significantly related to the development of adverse outcomes post-haplo-HSCT. Conclusions: These SNPs may play important roles in post-transplant immune response that can be considered during the selection of suitable donors.
Markers of Futile Resuscitation in Traumatic Hemorrhage: A Review of the Evidence and a Proposal for Futility Time-Outs during Massive Transfusion
Authors: Walsh Mark M. , Fox Mark D. , Moore Ernest E. , Johnson Jeffrey L. , Bunch Connor M. , Miller Joseph B. , Lopez-Plaza Ileana , Brancamp Rachel L. , Waxman Dan A. , Thomas Scott G. , Fulkerson Daniel H. , Thomas Emmanuel J. , Khan Hassaan A. , Zackariya Sufyan K. , Al-Fadhl Mahmoud D. , Zackariya Saniya K. , Thomas Samuel J. , Aboukhaled Michael W. ,   the Futile Indicators for Stopping Transfusion in Trauma (FISTT) Collaborative Group
Published: August 2024

The reduction in the blood supply following the 2019 coronavirus pandemic has been exacerbated by the increased use of balanced resuscitation with blood components including whole blood in urban trauma centers. This reduction of the blood supply has diminished the ability of blood banks to maintain a constant supply to meet the demands associated with periodic surges of urban trauma resuscitation. This scarcity has highlighted the need for increased vigilance through blood product stewardship, particularly among severely bleeding trauma patients (SBTPs). This stewardship can be enhanced by the identification of reliable clinical and laboratory parameters which accurately indicate when massive transfusion is futile. Consequently, there has been a recent attempt to develop scoring systems in the prehospital and emergency department settings which include clinical, laboratory, and physiologic parameters and blood products per hour transfused as predictors of futile resuscitation. Defining futility in SBTPs, however, remains unclear, and there is only nascent literature which defines those criteria which reliably predict futility in SBTPs. The purpose of this review is to provide a focused examination of the literature in order to define reliable parameters of futility in SBTPs. The knowledge of these reliable parameters of futility may help define a foundation for drawing conclusions which will provide a clear roadmap for traumatologists when confronted with SBTPs who are candidates for the declaration of futility. Therefore, we systematically reviewed the literature regarding the definition of futile resuscitation for patients with trauma-induced hemorrhagic shock, and we propose a concise roadmap for clinicians to help them use well-defined clinical, laboratory, and viscoelastic parameters which can define futility.
How to Improve Meniscal Repair through Biological Augmentation: A Narrative Review
Authors: Za Pierangelo , Ambrosio Luca , Vasta Sebastiano , Russo Fabrizio , Papalia Giuseppe Francesco , Vadalà Gianluca , Papalia Rocco
Published: August 2024

Since the role of the menisci in knee stability, proprioception, and homeostasis has been well established, significant efforts have been made to repair meniscal tears, resulting in excellent clinical outcomes and a reduction in the progression of knee osteoarthritis (OA). However, varying failure rates have been reported, raising questions regarding the healing potential in cases of complex injuries, poorly vascularized and degenerated areas, and generally in the presence of unfavorable biological characteristics. Therefore, over the last few decades, different strategies have been described to increase the chances of meniscal healing. Biological augmentation of meniscal repair through various techniques represents a safe and effective strategy with proven clinical benefits. This approach could reduce the failure rate and expand the indications for meniscal repair. In the present study, we thoroughly reviewed the available evidence on meniscal repair surgery and summarized the main techniques that can be employed to enhance the biological healing potential of a meniscal lesion. Our aim was to provide an overview of the state of the art on meniscal repair and suggest the best techniques to reduce their failure rate.
Advanced Capacitor-Based Battery Equalizer for Underwater Vehicles
Authors: Ho Kun-Che
Published: August 2024

As maritime technology advances, exploration of the oceans has progressively moved from surface exploration to underwater ventures. Unmanned underwater vehicles (UUVs), now prevalent for such exploration, effectively reduce human labor and lower operational costs. These vehicles rely on an internal Battery Storage System (BSS) that sustains device operation by extending operational duration and providing stable voltage. Typically arranged in series, BSSs face challenges due to differences in the chemical characteristics of individual batteries, which lead to discrepancies in battery voltages and cause imbalances during charge and discharge cycles. This results in varied utilization rates among the batteries and uneven aging of the battery pack, potentially decreasing operational efficiency and increasing failure rates, thus reducing reliability and safety. Considering the harsh environmental conditions and maintenance difficulties associated with underwater operations, this paper proposes a robust solution: a balancing system featuring a modular switch with electrical isolation. Through theoretical analysis and circuit simulation, this study constructs and tests a novel prototype of a capacitor-based equalizer circuit with electrical isolation, verifying its feasibility.
Phenotypic and Genotypic Characterization of Resistance and Virulence Markers in Candida spp. Isolated from Community-Acquired Infections in Bucharest, and the Impact of AgNPs on the Highly Resistant Isolates
Authors: Corbu Viorica Maria , Georgescu Ana-Maria , Marinas Ioana Cristina , Pericleanu Radu , Mogos Denisa Vasilica , Dumbravă Andreea Ștefania , Marinescu Liliana , Pecete Ionut , Vassu-Dimov Tatiana , Czobor Barbu Ilda , Csutak Ortansa , Ficai Denisa , Gheorghe-Barbu Irina
Published: August 2024

Background: This study aimed to determine, at the phenotypic and molecular levels, resistance and virulence markers in Candida spp. isolated from community-acquired infections in Bucharest outpatients during 2021, and to demonstrate the efficiency of alternative solutions against them based on silver nanoparticles (AgNPs). Methods: A total of 62 Candida spp. strains were isolated from dermatomycoses and identified using chromogenic culture media and MALDI-TOF MS, and then investigated for their antimicrobial resistance and virulence markers (VMs), as well as for metabolic enzymes using enzymatic tests for the expression of soluble virulence factors, their biofilm formation and adherence capacity on HeLa cells, and PCR assays for the detection of virulence markers and the antimicrobial activity of alternative solutions based on AgNPs. Results: Of the total of 62 strains, 45.16% were Candida parapsilosis; 29.03% Candida albicans; 9.67% Candida guilliermondii; 3.22% Candida lusitaniae, Candia pararugosa, and Candida tropicalis; and 1.66% Candida kefyr, Candida famata, Candida haemulonii, and Candida metapsilosis. Aesculin hydrolysis, caseinase, and amylase production were detected in the analyzed strains. The strains exhibited different indices of adherence to HeLa cells and were positive in decreasing frequency order for the LIP1, HWP1, and ALS1,3 genes (C. tropicalis/C. albicans). An inhibitory effect on microbial growth, adherence capacity, and on the production of virulence factors was obtained using AgNPs. Conclusions: The obtained results in C. albicans and Candida non-albicans circulating in Bucharest outpatients were characterized by moderate-to-high potential to produce VMs, necessitating epidemiological surveillance measures to minimize the chances of severe invasive infections.
Berberine Attenuates Acetamiprid Exposure-Induced Mitochondrial Dysfunction and Apoptosis in Rats via Regulating the Antioxidant Defense System
Authors: Phogat Annu , Singh Jagjeet , Sheoran Reena , Hasanpuri Arun , Chaudhary Aakash , Bhardwaj Shakti , Antil Sandeep , Kumar Vijay , Prakash Chandra , Malik Vinay
Published: August 2024

Acetamiprid (ACMP) is a neonicotinoid insecticide that poses a significant threat to the environment and mankind. Oxidative stress and mitochondrial dysfunction are considered prime contributors to ACMP-induced toxic effects. Meanwhile, berberine (BBR) a natural plant alkaloid, is a topic of interest because of its therapeutic and prophylactic actions. Therefore, this study evaluated the effects of BBR on ACMP-mediated alterations in mitochondrial functions and apoptosis in rat liver tissue. Male Wistar rats were divided into four groups: (I) control, (II) BBR-treated, (III) ACMP-exposed, and (IV) BBR+ACMP co-treated groups. The doses of BBR (150 mg/kg b.wt) and ACMP (1/10 of LD50, i.e., 21.7 mg/kg b.wt) were given intragastrically for 21 consecutive days. The results showed that the administration of ACMP diminished mitochondrial complex activity, downregulated complex I (ND1 and ND2) and complex IV (COX1 and COX4) subunit mRNA expression, depleted the antioxidant defense system, and induced apoptosis in rat liver. BBR pre-treatment significantly attenuated ACMP-induced mitochondrial dysfunction by maintaining mitochondrial complex activity and upregulating ND1, ND2, COX1, and COX4 mRNA expression. BBR reversed ACMP-mediated apoptosis by diminishing Bax and caspase-3 and increasing the Bcl-2 protein level. BBR also improved the mitochondrial antioxidant defense system by upregulating mRNA expression of PGC-1α, MnSOD, and UCP-2 in rat liver tissue. This study is the first to evaluate the protective potential of BBR against pesticide-induced mitochondrial dysfunction in liver tissue. In conclusion, BBR offers protection against ACMP-induced impairment in mitochondrial functions by maintaining the antioxidant level and modulating the apoptotic cascade.
Associations between Non-Essential Trace Elements in Women’s Biofluids and IVF Outcomes in Euploid Single-Embryo Transfer Cycles
Authors: Gonzalez-Martin Roberto , Palomar Andrea , Perez-Deben Silvia , Salsano Stefania , Quiñonero Alicia , Caracena Laura , Rucandio Isabel , Fernandez-Saavedra Rocio , Fernandez-Martinez Rodolfo , Conde-Vilda Estefania , Quejido Alberto J. , Giles Juan , Vidal Carmen , Bellver Jose , Dominguez Francisco
Published: August 2024

Previous studies have found inconsistent associations between heavy metals and metalloids (cadmium, lead, mercury, and arsenic), and reproductive outcomes. The biofluid concentrations of ten non-essential trace elements (Hg, Pb, As, Ba, Sr, Rb, Cs, Sn, Ni, and Co) were evaluated in 51 Spanish women undergoing ICSI, PGT-A, and SET/FET. Nine out of ten non-essential elements were detectable in follicular fluid, whole blood, and urine collected the day of vaginal oocyte retrieval (VOR) and the day of embryo transfer and then analyzed by ICP-MS or Tricell DMA-80 for mercury. Elevated mercury and strontium concentrations in follicular fluid were associated with poor ovarian response and preimplantation outcomes. Worst preimplantation outcomes were also identified in women with elevated whole-blood strontium or mercury, urinary arsenic, barium, and tin the day of VOR. High concentrations of urinary rubidium on VOR day were linked with enhanced fertilization and blastocyst development. Excessive titanium in whole blood was associated with lower odds of implantation, clinical pregnancy, and achieving a live birth in a given IVF cycle. Excessive urinary arsenic on the day of embryo transfer was associated with lower odds of live birth. Although these preliminary results need to be confirmed in larger populations, distinguishing organic and inorganic element forms, our findings show that some non-essential elements have a detrimental impact on human IVF outcomes.
From Technology to Traffic: How Website Technological Sophistication, Brand Recognition, and Business Model Innovation Drive Consumer Traffic in Korean E-Commerce
Authors: Yu Si , Liu Yutong , Hyun Eun-jung
Published: August 2024

As e-commerce continues to expand, understanding the factors that drive consumer traffic to business-to-consumer (B2C) websites is crucial. This study investigates the interplay between website technological sophistication, brand recognition, and business model innovation in influencing website traffic among Korean B2C companies. Drawing on data from 9003 companies across seven key sectors—finance, retail, healthcare, technology, food, education, and media—we employ Ordinary Least Squares (OLS) regression analysis to test our hypotheses. Our findings reveal that website technological sophistication is positively associated with monthly website visits. This relationship is particularly pronounced for companies with innovative business models, highlighting the synergistic effect of advanced website features and novel business strategies in attracting consumers. Conversely, the positive impact of website technological sophistication on traffic is less significant for well-established brands with high recognition levels, indicating that strong brand equity can mitigate the need for highly sophisticated websites. These results align with the Technology Acceptance Model (TAM), Innovation Diffusion Theory (IDT), and Signaling Theory (ST), providing a nuanced understanding of how technology, branding, and innovation intersect to drive online consumer behavior. Our study offers valuable insights for e-commerce firms seeking to optimize their digital presence and underscores the importance of investing in advanced website functionalities, particularly for lesser-known brands and companies with innovative business models. Future research should explore these dynamics in different cultural and industry contexts to enhance the generalizability of our findings.
How Does Urban Scale Influence Carbon Emissions?
Authors: Yang Jiayu , Feng Xinhui , Li Yan , He Congying , Wang Shiyi , Li Feng
Published: August 2024

Low-carbon cities aim to minimize greenhouse gas emissions in the context of climate change in the process of urbanization. Maintaining these cities at an appropriate physical scale has been proven to contribute to carbon reduction. Therefore, this study extended the definition of the city scale to an integrated framework with three dimensions: the construction land area, population, and economy. The urban construction land of 258 cities in China during 2012 to 2019 was divided into commercial, industrial, residential, and traffic sectors, and carbon emissions were calculated for each. The regression relationship between carbon emissions and the urban scale revealed by panel data analysis showed the following conclusions: (1) carbon emissions were concentrated in north China, provincial capital cities, and municipalities directly under the central government during the research period, and the industrial sector was the main emission resource, accounting for more than 85% of the total emissions. (2) Carbon emissions per unit of land decreased with the increasing land scale, regardless of the land-use type. The growth rate of carbon emissions was slower than that of the population, and cities also became more efficient as their economic scale expanded. (3) Compared with small cities, the large ones benefited more from increasing commercial and traffic land areas, whereas industrial emissions for production needs exhibited significant agglomeration characteristics. Overall, low-carbon planning should focus on the driving role of provincial capital cities as large cities tend to be more efficient, and develop the emission reduction potential of major industrial cities as well.
The Identification of Historic Plant Landscape Characteristics and Conservation Strategies for Longevity Hill Based on the WSL Monoplotting Tool
Authors: Wu Jingyu , Xiao Yao , Zhu Linjie , Cheng Sihua
Published: August 2024

The surrounding environment of architectural heritage sites is integral to cultural heritage protection; plant landscapes play crucial roles in them. Controlling plant spaces and appearances is essential for preserving plant landscapes. A World Cultural Heritage Site, the Summer Palace has undergone multiple changes since the 1860s; restoring and protecting plant landscapes has been an ongoing research focus. However, data accuracy limitations have hindered analyses of the overall spatial characteristics of historical gardens. Here, the historical dynamics and unique landscape features of plants on the front hill of Longevity Hill (FLH) are explored, and conservation and renewal strategies are proposed. Geographic information system (GIS) and WSL Monoplotting Tool are used to identify historical plant spaces. Plant space types are classified, and their landscape characteristics are analyzed. On the basis of historical events, the historical plant spaces on the FLH can be divided into two major categories and six subcategories. The vegetation retention area (south side) was less affected, and the plant landscape along Kunming Lake was the most well-preserved. However, the vegetation-damaged area (north side) was impacted more in the western part than in the eastern part, with notable changes in spatial landscape characteristics, particularly regarding forest function, morphology, and structure. Strategies are proposed for reducing human intervention and adjusting retention areas; furthermore, historical images and spatial grading in damaged areas can be used to suggest landscape adjustment and restoration strategies. This study introduces a method for analyzing the historical characteristics of plant landscapes over time that can be used to protect cultural heritage sites worldwide.
Coupled Coordination and the Spatial Connection Network Analysis of New Urbanization and Ecological Resilience in the Urban Agglomeration of Central Guizhou, China
Authors: Wu Chengmin , Ren Haili
Published: August 2024

This study evaluates the new urbanization (NU) quality and the ecological resilience (ER) of 33 districts and counties in the Urban Agglomeration of Central Guizhou from 2010 to 2020. For this purpose, we used a modified coupled coordination degree (CCD) model, spatial autocorrelation analysis, and trend surface analysis to analyze the spatiotemporal evolutionary characteristics of the CCD of NU and ER. Meanwhile, we used a modified gravity model and social network analysis to investigate the spatial connection network (SCN) characteristics of the CCD of NU and ER. The results show that (1) the general NU quality has increased significantly in the Urban Agglomeration of Central Guizhou. There is, however, a downward trend in ER. (2) For the CCD of NU and ER in the Urban Agglomeration of Central Guizhou, there is coupling dissonance, with a double U-shaped arc, characterized by west > north > south > east > central. (3) The network density increases and then decreases. Network connectivity is 1, and network efficiency decreases and then increases. (4) During the study period, the SCN is characterized by significant core–edge characteristics; there are no “island nodes” in the SCN.
A New Framework of Land Use Simulation for Land Use Benefit Optimization Based on GMOP-PLUS Model—A Case Study of Haikou
Authors: Fu Hui , Liang Yaowen , Chen Jie , Zhu Ling , Fu Guang
Published: August 2024

Multi-scenario simulation and prediction of land use can provide guidance for the optimization of land use patterns. Combining the GMOP model with the PLUS model can better evaluate the influence of different land use strategies on the comprehensive benefits of land use and improve the scientificity of the simulation results. This study takes Haikou City as the research area. As the political, economic, and cultural center of Hainan Province, it is the highest urbanization area in Hainan Province and also the vane of the urban development of Hainan Province. Its development experience and model play an important leading role in the surrounding cities. The land use data of 2010, 2015, and 2020 were selected, and the spatiotemporal pattern of land use under the 2035 Business As Usual scenario (BAU), Economic Development scenario (ED), and Economic and Ecological Balanced Development scenario (EEB) was simulated based on the GMOP-PLUS model. The results show that: (1) The prediction results generally show the trend of the decrease in cultivated land and forest land and the increase in construction land, among which the expansion capacity of construction land is the strongest, and the forest land is more occupied, but the increase and decrease in land use types are different under different scenarios. (2) The three simulation scenarios all show the trend of economic benefit improvement and ecological benefit decline, which indicates that the primary objective of Haikou City’s future development remains focused on economic construction, with the potential compromise of ecological functions to accommodate urban expansion. (3) The comprehensive benefits of the region in the EEB scenario are significantly higher than those in the BAU and ED scenarios. The optimized land use structure is more balanced, the scale of urban expansion is limited, and the loss of important ecological land is reduced to a minimum, which is more in line with the current concept of sustainable development. The study can serve as a reference for the coordinated development of urban planning, land use management, and ecological environment in Haikou.
Effect of FluoRoquinolones on Aortic Growth, aortic stIffness and wave refLEctionS (FRAGILES study)
Authors: Gardikioti Vasiliki , Georgakopoulos Christos , Solomou Eirini , Lazarou Emilia , Fasoulakis Konstantinos , Terentes-Printzios Dimitrios , Tsioufis Konstantinos , Iliopoulos Dimitrios , Vlachopoulos Charalambos
Published: August 2024

Background: The widespread use of fluoroquinolones has been associated with the formation, dissection, and rupture of aortic aneurysms. Arterial biomarkers are established predictors of cardiovascular events. The present study was designed to investigate the effect of quinolones on arterial stiffness and aortic size for the first time. Methods: We studied 28 subjects receiving short-term (<15 days) antibiotic therapy involving quinolones and 27 age- and sex-matched subjects receiving an alternative to quinolone antibiotics. The follow-up period was approximately 2 months. The study’s primary endpoint was the carotid–femoral pulse wave velocity (cfPWV) difference between the two groups 2 months after therapy initiation. Secondary endpoints were the augmentation index corrected for heart rate (AIx@75) and sonographically assessed aortic diameters 2 months after the initial treatment. Results: Subjects had similar values of arterial biomarkers, blood pressure measurements, and aortic diameters at baseline. At follow-up, no significant change was observed between the two groups regarding the hemodynamic parameters and arterial biomarkers (p > 0.05 for all), i.e., cfPWV (7.9 ± 2.6 m/s for the control group vs. 8.1 ± 2.4 m/s for the fluoroquinolones group; p = 0.79), AIx@75 (22.6 ± 9.0% for the control group vs. 26.6 ± 8.1% for the fluoroquinolones group; p = 0.09), and aortic diameters. Conclusions: To our knowledge, FRAGILES is the first study to provide insights into the possible effects of fluoroquinolones on arterial biomarkers, showing that, at least in the short term, treatment with fluoroquinolones does not affect aortic function and diameter.
Personalized Management of Patients with Proliferative Diabetic Vitreoretinopathy
Authors: Ecsedy Monika , Szabo Dorottya , Szilagyi Zsuzsa , Nagy Zoltan Zsolt , Recsan Zsuzsanna
Published: August 2024

Purpose: To evaluate prognostic factors for visual outcome in patients with diabetes who have undergone vitrectomy (PPV) for severe proliferative diabetic vitreoretinopathy (PDVR) in at least one eye in the past 15 years. Methods: Medical records of 132 eyes of 66 patients were analyzed (median age 52 years 21–80; patients with type 1/2 diabetes 40/26; median follow-up 38 months 9–125). Correlations between final favorable visual outcome defined as 0.5≤ best-corrected visual acuity (BCVA) and prognostic factors (age, sex, type and duration of diabetes, metabolic status, BCVA, diabetic retinopathy status, data of preoperative management, data of vitrectomy, and postoperative complications) were analyzed. Results: BCVA improved significantly in the entire study cohort (from median 0.05 min–max 0.001–1 to 0.32, 0.001–1, p < 0.001). Visual stabilization was achieved in the majority of patients, and good visual acuity (0.5 ≤ BCVA) was maintained in more than one-third of the eyes. Multivariable GEE statistics showed that in addition to the duration of diabetes and stable HbA1c values, only preoperative tractional macular detachment proved to be an independent significant predictor of visual outcome. Conclusions: Pars plana vitrectomy is a useful tool when performed early before tractional macular detachment. However, long-term visual stability can only be achieved with good metabolic control.
Outcomes of Birmingham Hip Resurfacing Based on Clinical Aspects and Retrieval Analysis of Failed Prosthesis
Authors: Antoniac Iulian , Valeanu Niculae , Niculescu Marius , Antoniac Aurora , Robu Alina , Popescu Larisa , Manescu (Paltanea) Veronica , Anusca Dan , Enachescu Catalin Ionel
Published: August 2024

This research aims to identify the prevalence of failure for Birmingham Hip Prosthesis (BHR) in total hip arthroplasty and to analyze its reasons from biomaterials and biofunctional perspectives. We present our current analysis and tests on a series of different BHR-retrieved prostheses after premature failure. Relevant clinical data, such as X-ray investigations and intraoperative images for clinical case studies, were analyzed to better understand all factors involved in BHR prosthesis failure. A detailed analysis of the failures highlighted uneven cement distribution, overloading in certain areas, and void formation in the material. A closer investigation using microscopical techniques revealed the presence of a crack originating from the gap between the cement mantle and human bone. Additionally, scanning electron microscopy analyses were conducted as part of the investigation to examine bone cement morphology in detail and better understand the interactions at the interfaces between implant, cement, and bone. In conclusion, this research emphasizes the importance of surgical technique planning and the cementation procedure in the success rate of BHR prostheses. It also underscores the need to carefully evaluate patient characteristics and bone quality to minimize the risk of BHR prosthesis failure. The cementation procedure seems to be essential for the long-term functionality of the BHR prosthesis.
Preparation and Properties of Lightweight Aggregates from Discarded Al2O3-ZrO2-C Refractories
Authors: Sun Shuli , Qu Junfeng , Sun Mengyong , Ren Xinming , Gong Cheng , Mu Xin , Zan Wenyu , Zhou Zhangyan , Deng Chengji , Ma Beiyue
Published: August 2024

Refractory materials are an important pillar for the stable development of the high-temperature industry. A large amount of waste refractories needs to be further disposed of every year, so it is of great significance to carry out research on the recycling of used refractories. In this work, lightweight composite aggregate was prepared by using discarded Al2O3-ZrO2-C refractories as the main raw material, and the performance of the prepared lightweight aggregate was improved by adjusting the calcination temperature and introducing light calcined magnesia additives. The results showed that the cold compressive strength and thermal shock resistance of the lightweight aggregates were significantly improved with increasing calcination temperature. Moreover, the introduction of light calcined magnesia can effectively improve the apparent porosity, cold compressive strength, and thermal shock resistance of the prepared lightweight aggregates at the calcination temperature of 1400 °C. Consequently, this work provides a useful reference for the resource utilization of used refractories, while the prepared lightweight aggregates are expected to be applied in the field of high-temperature insulation.
Experimental Evaluation of Compressive Properties of Early-Age Mortar and Concrete Hollow-Block Masonry Prisms within Construction Stages
Authors: Abasi Ali , Banting Bennett , Sadhu Ayan
Published: August 2024

Early-age masonry structures require temporary support until they achieve full strength. Nevertheless, there is a limited understanding of the properties of freshly laid masonry and the design of newly constructed, unsupported masonry walls. This situation has led to numerous instances of structural damage and injuries to workers, prompting conservative construction bracing techniques. This paper presents comprehensive experimental studies on early-age mortar cubes and masonry prisms to assess the effects of curing time on the compressive properties of masonry assemblies, which is necessary for the design of temporary bracing. The change in modulus of elasticity and compressive strength of masonry prisms and mortar with curing time has been experimentally assessed. The results indicate that the compressive strength of freshly cast mortar cubes is relatively insignificant until approximately 24 h after construction, when it was observed to increase logarithmically. Regarding the performance perspective, the compressive strength of early-age masonry prisms is inconsiderable, less than 15% of full strength during the first day after construction. By contrast, regarding the life safety perspective, the compressive properties of a mortar joint within a masonry assembly (which is of more practical interest) appear to have no effect on the failure strength of concrete masonry prisms over the range of ages tested. The failure modes of the early-age mortar cubes and early-age masonry prism samples depend on the curing time, and different failure modes occurred before and after the start of the primary hydration phase, which is 20.8 h after construction. It is anticipated that the proposed research will provide valuable material properties leading to efficient design of control devices (e.g., temporary bracing) and improved guidelines for concrete-block masonry construction.
Probiotic Lactobacilli Ameliorate Antibiotic-Induced Cognitive and Behavioral Impairments in Mice
Authors: Yarullina Dina , Novoselova Vera , Alexandrova Anastasia , Arslanova Alisa , Yakovleva Olga , Shaidullov Ilnar , Nikolaev Yury , El-Registan Galina , Kudrin Vladimir , Sitdikova Guzel
Published: August 2024

Increasing evidence suggests that the gut microbiota, through the “microbiota–gut–brain axis”, can regulate anxiety, mood, and cognitive abilities such as memory and learning processes. Consistently with this, treatments altering the gut microbiota, such as antibiotics and probiotics, may influence brain function and impact behavior. The mechanisms that underlie the interplay between the intestinal microbiota and the brain have been intensively studied. We aimed to investigate the effects of two probiotic lactobacilli strains, Lacticaseibacillus rhamnosus 12L and Lactiplantibacillus plantarum 8PA3, on behavioral disorders in mice induced by a two-week parenteral treatment with broad-spectrum antibiotics. On completion of the treatment, the mice were subjected to behavioral tests, including the open field test (OFT), novel object recognition test (ORT), and T-maze test. Antibiotic-treated mice demonstrated anxiety-related behavior, decreased cognition, and retarded exploratory activity that were ameliorated by the administration of probiotics. As was determined by high-performance liquid chromatography (HPLC), both tested strains produced serotonin and its metabolite 5-hydroxyindoleacetic acid (5-HIAA), as well as dopamine, which was further metabolized into norepinephrine by L. plantarum 8PA3 and epinephrine by L. rhamnosus 12L. Moreover, these lactobacilli were found to harbor catecholamines and 3,4-dihydroxyphenylacetic acid (DOPAC) in their biomass when grown on MRS broth. Additionally, L. plantarum 8PA3 and L. rhamnosus 12L were able to impact oxidative stress via H2O2 production and antioxidant activity, as determined in this study by the ferrous oxidation–xylenol orange (FOX) assay and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, respectively. The results obtained in this study support the role of probiotics as a promising therapeutic for neurological disorders. However, more investigations are required to confirm the clinical significance of this finding.
Comparison of Biofilm Growth and Quorum Sensing Molecules in Vaginal Lactobacillus Species: A Preliminary Report
Authors: Sanchez Asley , Alimiran Fnu , Gandhi Kushal , David Samuel , Babayco Christopher B. , Fiveash Chloe , Garza John , Le Duc , Le Triet , Oldham Athenia , Henderson Douglas , Galloway Michael , Ventolini Gary
Published: August 2024

Recently, studies suggest that the protective effects of Lactobacillus within the female reproductive tract may be partly due to their ability to form biofilms. This study aims to explore the possibility that Lactobacillus can produce key mediators to further bolster the survival of biofilms in human vaginal microbiomes. Three bacterial species, namely, Lactobacillus gasseri, L. crispatus, and L. jensenii, sourced from human female subjects were used to carry out experiments examining the growth of biofilms using a microfermenter system. The bacteria were used to inoculate a glass rod spatula which was subsequently transferred to the microfermenter system. The resulting biofilm growing on the glass spatula was harvested in media and stored in a −80 °C freezer for gas chromatography–mass spectroscopy analysis. We found that quorum sensing compounds, acyl homoserine lactones (AHLs), were detected in the biofilm of L. crispatus and L. jensenii, but none were detected in L. gasseri. The biofilm produced by L. crispatus and L. jensenii was much higher in quantity than the biofilm produced by L. gasseri. Aside from oligopeptides quorum sensing, lactobacilli were found to also have AHL compounds that may help them produce more biofilms and improve the survival and growth of their bacterial communities in the female genital area.