bluuebunny/crossref_metadata_2025 huggingface dataset:
Phylogenetically Distant BABY BOOM Genes From Setaria italica Induce Parthenogenesis in Rice
Authors: Chahal Lovepreet Singh , Conner Joann A. , Ozias-Akins Peggy
Published: July 2022

The combination of apomixis and hybrid production is hailed as the holy grail of agriculture for the ability of apomixis to fix heterosis of F1 hybrids in succeeding generations, thereby eliminating the need for repeated crosses to produce F1 hybrids. Apomixis, asexual reproduction through seed, achieves this feat by circumventing two processes that are fundamental to sexual reproduction (meiosis and fertilization) and replacing them with apomeiosis and parthenogenesis, resulting in seeds that are clonal to the maternal parent. Parthenogenesis, embryo development without fertilization, has been genetically engineered in rice, maize, and pearl millet using PsASGR-BABY BOOM-like (PsASGR-BBML) transgenes and in rice using the OsBABY BOOM1 (OsBBM1) cDNA sequence when expressed under the control of egg cell-specific promoters. A phylogenetic analysis revealed that BABY BOOM (BBM)/BBML genes from monocots cluster within three different clades. The BBM/BBML genes shown to induce parthenogenesis cluster within clade 1 (the ASGR-BBML clade) along with orthologs from other monocot species, such as Setaria italica. For this study, we tested the parthenogenetic potential of three BBM transgenes from S. italica, each a member of a different phylogenetic BBM clade. All transgenes were genomic constructs under the control of the AtDD45 egg cell-specific promoter. All SiBBM transgenes induced various levels of parthenogenetic embryo development, resulting in viable haploid T1 seedlings. Poor seed set and lower haploid seed production were characteristics of multiple transgenic lines. The results presented in this study illustrate that further functional characterization of BBMs in zygote/embryo development is warranted.
Phylotranscriptomics Illuminates the Placement of Whole Genome Duplications and Gene Retention in Ferns
Authors: Pelosi Jessie A. , Kim Emily H. , Barbazuk W. Brad , Sessa Emily B.
Published: July 2022

Ferns are the second largest clade of vascular plants with over 10,000 species, yet the generation of genomic resources for the group has lagged behind other major clades of plants. Transcriptomic data have proven to be a powerful tool to assess phylogenetic relationships, using thousands of markers that are largely conserved across the genome, and without the need to sequence entire genomes. We assembled the largest nuclear phylogenetic dataset for ferns to date, including 2884 single-copy nuclear loci from 247 transcriptomes (242 ferns, five outgroups), and investigated phylogenetic relationships across the fern tree, the placement of whole genome duplications (WGDs), and gene retention patterns following WGDs. We generated a well-supported phylogeny of ferns and identified several regions of the fern phylogeny that demonstrate high levels of gene tree–species tree conflict, which largely correspond to areas of the phylogeny that have been difficult to resolve. Using a combination of approaches, we identified 27 WGDs across the phylogeny, including 18 large-scale events (involving more than one sampled taxon) and nine small-scale events (involving only one sampled taxon). Most inferred WGDs occur within single lineages (e.g., orders, families) rather than on the backbone of the phylogeny, although two inferred events are shared by leptosporangiate ferns (excluding Osmundales) and Polypodiales (excluding Lindsaeineae and Saccolomatineae), clades which correspond to the majority of fern diversity. We further examined how retained duplicates following WGDs compared across independent events and found that functions of retained genes were largely convergent, with processes involved in binding, responses to stimuli, and certain organelles over-represented in paralogs while processes involved in transport, organelles derived from endosymbiotic events, and signaling were under-represented. To date, our study is the most comprehensive investigation of the nuclear fern phylogeny, though several avenues for future research remain unexplored.
Chloroplastic photoprotective strategies differ between bundle sheath and mesophyll cells in maize (Zea mays L.) Under drought
Authors: Liu Wen-Juan , Liu Hao , Chen Yang-Er , Yin Yan , Zhang Zhong-Wei , Song Jun , Chang Li-Juan , Zhang Fu-Li , Wang Dong , Dai Xiao-Hang , Wei Chao , Xiong Mei , Yuan Shu , Zhao Jun
Published: July 2022

Bundle sheath cells play a crucial role in photosynthesis in C4 plants, but the structure and function of photosystem II (PSII) in these cells is still controversial. Photoprotective roles of bundle sheath chloroplasts at the occurrence of environmental stresses have not been investigated so far. Non-photochemical quenching (NPQ) of chlorophyll a fluorescence is the photoprotective mechanism that responds to a changing energy balance in chloroplasts. In the present study, we found a much higher NPQ in bundle sheath chloroplasts than in mesophyll chloroplasts under a drought stress. This change was accompanied by a more rapid dephosphorylation of light-harvesting complex II (LHCII) subunits and a greater increase in PSII subunit S (PsbS) protein abundance than in mesophyll cell chloroplasts. Histochemical staining of reactive oxygen species (ROS) suggested that the high NPQ may be one of the main reasons for the lower accumulation of ROS in bundle sheath chloroplasts. This may maintain the stable functioning of bundle sheath cells under drought condition. These results indicate that the superior capacity for dissipation of excitation energy in bundle sheath chloroplasts may be an environmental adaptation unique to C4 plants.
Genome-Wide Analysis of Multidrug and Toxic Compound Extruction Transporters in Grape
Authors: Watanabe Megumi , Otagaki Shungo , Matsumoto Shogo , Shiratake Katsuhiro
Published: July 2022

Grape (Vitis vinifera L.) is an important fruit crop in the world. It is used as a table grape and is also used for raisin and wine production. Grape berries accumulate secondary metabolites, such as anthocyanins, tannins, and resveratrol, which are known as functional compounds for human health. Multidrug and toxic compound extrusion transporter (MATEs) transport secondary metabolites. MATEs also transport other solutes, including organic acids, and toxic xenobiotics, depending on cation gradient and play various roles in plants. MATE comprises 300–500 amino acid residues and possesses a MATE domain and 8–12 transmembrane domains. In the present study, 59 MATE genes were identified in the grape genome, and phylogenetic analysis revealed the presence of four groups of grape MATEs (Group 1–4). Their information, such as gene structures, protein motifs, predicted subcellular localizations, and gene IDs of four genome annotations, that is, CRIBI v1, CRIBI v2, Genoscope, and Vcost v3, were annotated. The transport substrates and physiological functions of grape MATEs were estimated based on their homology with the analyzed MATEs in other plant species. Group 1 may transport toxic compounds and alkaloids, Group 2 may transport polyphenolic compounds, Group 3 may transport organic acids, and Group 4 may transport plant hormones related to signal transduction. In addition to the known anthocyanin transporters, VvMATE37 and VvMATE39, a novel anthocyanin transporter, VvMATE38 in Group 2, was suggested as a key transporter for anthocyanin accumulation in grape berry skin. VvMATE46, VvMATE47, and VvMATE49 in Group 3 may contribute to Al3+ detoxification and Fe2+/Fe3+ translocation via organic acid transport. This study provides helpful and fundamental information for grape MATE studies and resolves the confusion of gene IDs in different genome annotations.
Generating Douglas-fir Breeding Value Estimates Using Airborne Laser Scanning Derived Height and Crown Metrics
Authors: du Toit Francois , Coops Nicholas C. , Ratcliffe Blaise , El-Kassaby Yousry A.
Published: July 2022

Progeny test trials in British Columbia are essential in assessing the genetic performance via the prediction of breeding values (BVs) for target phenotypes of parent trees and their offspring. Accurate and timely collection of phenotypic data is critical for estimating BVs with confidence. Airborne Laser Scanning (ALS) data have been used to measure tree height and structure across a wide range of species, ages and environments globally. Here, we analyzed a Coastal Douglas-fir [Pseudotsuga menziesii var. menziesii (Mirb.)] progeny test trial located in British Columbia, Canada, using individual tree high-density Airborne Laser Scanning (ALS) metrics and traditional ground-based phenotypic observations. Narrow-sense heritability, genetic correlations, and BVs were estimated using pedigree-based single and multi-trait linear models for 43 traits. Comparisons of genetic parameter estimates between ALS metrics and traditional ground-based measures and single- and multi-trait models were conducted based on the accuracy and precision of the estimates. BVs were estimated for two ALS models (ALSCAN and ALSACC) representing two model-building approaches and compared to a baseline model using field-measured traits. The ALSCAN model used metrics reflecting aspects of vertical distribution of biomass within trees, while ALSACC represented the most statistically accurate model. We report that the accuracy of both the ALSCAN (0.8239) and ALSACC (0.8254) model-derived BVs for mature tree height is a suitable proxy for ground-based mature tree height BVs (0.8316). Given the cost efficiency of ALS, forest geneticists should explore this technology as a viable tool to increase breeding programs’ overall efficiency and cost savings.
Distributed Averaging Problems of Agriculture Picking Multi-Robot Systems via Sampled Control
Authors: Ma Fengying , Yao Hui , Du Mingjun , Ji Peng , Si Xiaoan
Published: July 2022

Distributed control of agriculture picking multi-robot systems has been widely used in the field of smart agriculture, this paper aims to explore the distributed averaging problems of agriculture picking multi-robot systems under directed communication topologies by taking advantage of the sampled data. With the algebraic graph theory concepts and the matrix theory, a distributed protocol is proposed based on the nearest sampled neighbor information. It is shown that under the proposed protocol, the states of all agents can be guaranteed to reach average consensus whose value is the averaging of the initial states of all agents. Besides, when considering time-delay, the other distributed protocol is constructed, in which a time margin of the time-delay can be determined simultaneously. The necessary and sufficient consensus results can be developed even though the time delay exists. Simulation results are given to demonstrate the effectiveness of our developed consensus results.
Research Progress on Genetic Basis of Fruit Quality Traits in Apple (Malus × domestica)
Authors: Liu Wenjun , Chen Zijing , Jiang Shenghui , Wang Yicheng , Fang Hongcheng , Zhang Zongying , Chen Xuesen , Wang Nan
Published: July 2022

Identifying the genetic variation characteristics of phenotypic traits is important for fruit tree breeding. During the long-term evolution of fruit trees, gene recombination and natural mutation have resulted in a high degree of heterozygosity. Apple (Malus × domestica Borkh.) shows strong ecological adaptability and is widely cultivated, and is among the most economically important fruit crops worldwide. However, the high level of heterozygosity and large genome of apple, in combination with its perennial life history and long juvenile phase, complicate investigation of the genetic basis of fruit quality traits. With continuing augmentation in the apple genomic resources available, in recent years important progress has been achieved in research on the genetic variation of fruit quality traits. This review focuses on summarizing recent genetic studies on apple fruit quality traits, including appearance, flavor, nutritional, ripening, and storage qualities. In addition, we discuss the mapping of quantitative trait loci, screening of molecular markers, and mining of major genes associated with fruit quality traits. The overall aim of this review is to provide valuable insights into the mechanisms of genetic variation and molecular breeding of important fruit quality traits in apple.
Integrated Physiochemical, Hormonal, and Transcriptomic Analysis Revealed the Underlying Mechanisms for Granulation in Huyou (Citrus changshanensis) Fruit
Authors: Kang Chen , Jiang Anze , Yang Han , Zheng Guixia , Wang Yue , Cao Jinping , Sun Chongde
Published: July 2022

Juice sac granulation is a common internal physiological disorder of citrus fruit. In the present study, we compared the physiochemical characteristics and transcriptome profiles of juice sacs in different granulation levels from Huyou fruit (Citrus changshanensis). The accumulation of cell wall components, including the water-soluble pectin, protopectin, cellulose, and lignin, were significantly correlated with the granulation process, resulting in the firmness increase of the juice sac. The in situ labeling of the cell wall components indicated the early accumulation of cellulose and high-methylesterified pectin in the outer layer cells, as well as the late accumulation of lignin in the inner layer cells of the juice sac. Several phytohormones, including auxins, abscisic acids, cytokinins, jasmonic acid, salicylic acid, and/or their metabolites, were positively correlated to the granulation level, indicating an active and complex phytohormones metabolism in the granulation process. Combining the trend analysis by the Mfuzz method and the module-trait correlation analysis by the Weighted Gene Co-expression Network Analysis method, a total of 2940 differentially expressed genes (DEGs) were found to be positively correlated with the granulation level. Gene Ontology (GO) enrichment indicated that the selected DEGs were mainly involved in the cell wall organization and biogenesis, cell wall macromolecule metabolic process, carbohydrate metabolic process, and polysaccharide metabolic process. Among these selected genes, those encoding β-1,4-xylosyltransferase IRX9, cellulose synthase, xyloglucan: xyloglucosyl transferase, xyloglucan galactosyltransferase MUR3, α-1,4-galacturonosyltransferase, expansin, polygalacturonase, pectinesterase, β-glucosidase, β-galactosidase, endo-1,3(4)-β-glucanase, endoglucanase and pectate lyase that required for the biosynthesis or structural modification of cell wall were identified. In addition, NAC, MYB, bHLH, and MADS were the top abundant transcription factors (TFs) families positively correlated with the granulation level, while the LOB was the top abundant TFs family negatively correlated with the granulation level.
Panax notoginseng WRKY Transcription Factor 9 Is a Positive Regulator in Responding to Root Rot Pathogen Fusarium solani
Authors: Zheng Lilei , Qiu Bingling , Su Linlin , Wang Hanlin , Cui Xiuming , Ge Feng , Liu Diqiu
Published: July 2022

Panax notoginseng (Burk) F.H. Chen is a rare and valuable Chinese herb, but root rot mainly caused by Fusarium solani severely affects the yield and quality of P. notoginseng herbal materials. In this study, we isolated 30 P. notoginseng WRKY transcription factors (TFs), which were divided into three groups (I, II, and III) on the basis of a phylogenetic analysis. The expression levels of 10 WRKY genes, including PnWRKY9, in P. notoginseng roots increased in response to a methyl jasmonate (MeJA) treatment and the following F. solani infection. Additionally, PnWRKY9 was functionally characterized. The PnWRKY9 protein was localized to the nucleus. The overexpression of PnWRKY9 in tobacco (Nicotiana tabacum) considerably increased the resistance to F. solani, whereas an RNAi-mediated decrease in the PnWRKY9 expression level in P. notoginseng leaves increased the susceptibility to F. solani. The RNA sequencing and hormone content analyses of PnWRKY9-overexpression tobacco revealed that PnWRKY9 and the jasmonic acid (JA) signaling pathway synergistically enhance disease resistance. The PnWRKY9 recombinant protein was observed to bind specifically to the W-box sequence in the promoter of a JA-responsive and F. solani resistance-related defensin gene (PnDEFL1). A yeast one-hybrid assay indicated that PnWRKY9 can activate the transcription of PnDEFL1. Furthermore, a co-expression assay in tobacco using β-glucuronidase (GUS) as a reporter further verified that PnWRKY9 positively regulates PnDEFL1 expression. Overall, in this study, we identified P. notoginseng WRKY TFs and demonstrated that PnWRKY9 positively affects plant defenses against the root rot pathogen. The data presented herein provide researchers with fundamental information regarding the regulatory mechanism mediating the coordinated activities of WRKY TFs and the JA signaling pathway in P. notoginseng responses to the root rot pathogen.
BnaA03.ANS Identified by Metabolomics and RNA-seq Partly Played Irreplaceable Role in Pigmentation of Red Rapeseed (Brassica napus) Petal
Authors: Hao Pengfei , Liu Han , Lin Baogang , Ren Yun , Huang Lan , Jiang Lixi , Hua Shuijin
Published: July 2022

Colorful flowers of rapeseed (Brassica napus L.) have been a hotspot for researchers, but the underlying mechanisms of pigment formation still need to be clarified. In this study, two stages of unopened rapeseed petals with red, white, and yellow colors were selected to identify the metabolites and genes involved in red pigment formation. Metabolomic analysis showed that flavonoids enriched the most co-differentially accumulated metabolites among all categories, and showed higher accumulation in red petal rapeseed than in white and yellow petal ones. RNA-seq analysis showed that among co-differentially expressed genes involved in red pigment formation, genes involved in anthocyanin (belonging to flavonoids) biosynthesis pathway were largely regulated by ANS, DFR, and UF3GT. The expression of those genes was higher in red petals of rapeseed than in white and yellow petals ones as well. Results of RNA interference of BnaA03.ANS in red rapeseed altered petal colors from raspberry red to beige red and zinc yellow under different interference levels, with the contents of pelargonidin, cyanidin, lutein, neoxanthin, β-carotene, and lycopene significantly decreased. However, overexpression of BnaA03.ANS in yellow rapeseed petals did not change the color of yellow petals. This study confirmed the important function of flavonoids, especially anthocyanins on red pigment formation, and for the first time, identified the irreplaceable role of BnaA03.ANS on red-flowered rapeseed.
Identification of Fusarium verticillioides Resistance Alleles in Three Maize Populations With Teosinte Gene Introgression
Authors: Feng Xuanjun , Xiong Hao , Zheng Dan , Xin Xiaobing , Zhang Xuemei , Wang Qingjun , Wu Fengkai , Xu Jie , Lu Yanli
Published: July 2022

Fusarium ear rot (FER) is a common fungal disease in maize (Zea mays L.) caused by Fusarium verticillioides. Resistant germplasm resources for FER are rare in cultivated maize; however, teosintes (Z. mays ssp. parviglumis and Z. mays ssp. diploperennis), which are wild-type species of maize, have the potential to offer a novel source of resistance alleles to enhance pathogen resistance in modern maize. Therefore, the aim of this study was to identify favorable alleles that confer significant levels of resistance toward FER. Three populations of BC2F8 recombinant inbred lines (RILs) were developed by crossing two different teosintes, Z. diploperennis and Z. parviglumis, with maize inbred lines B73 and Zheng58, and were screened for FER resistance. We found that Z. diploperennis and Z. parviglumis had higher resistance toward F. verticillioides in the leaves than B73 and Zheng58. However, the resistance toward F. verticillioides in the leaf and ear was unrelated among RILs. FER resistance was positively correlated with grain yield in the B73 × diploperennis (BD) and Zheng58 × parviglumis (ZP) populations, partly because the quantitative trait loci (QTLs) of FER resistance and yield traits were located close together. Four coincident QTLs (qFERbd5.177, qFERbd10.140, qFERzp4.066, and qFERzp5.116) and two highly reliable resistance-yield synergistic QTLs (qFERbd10.140 and qFERzp4.066) were identified in the BD and ZP populations, opening up the possibility of breeding for FER resistance without reducing yield.
Protecting the Ingroup? Authoritarianism, Immigration Attitudes, and Affective Polarization
Authors: Renström Emma A. , Bäck Hanna , Carroll Royce
Published: July 2022

What makes people affectively polarized? Affective polarization is based on the idea that partisanship can be a social identity leading to polarization in the form of intergroup distancing between the own party and the other parties. In this study, we argue that perceived threats from an outgroup can spur affective polarization. To investigate this, we use the issue of immigration, often framed as a threat by right-wing groups, to examine whether individual-level differences influence how sensititivity to the perception of immigration as a threat. One such factor is the trait right-wing authoritarianism (RWA), which is characterized by emphasis on submission to authority and upholding norms of social order. The emphasis among individuals with this trait on protecting the ingroup from threats means that negativity toward immigration is likely to extend toward political opponents, resulting in an increase in affective polarization. Thus, we hypothesize that the affective polarization is likely to increase when individuals interpret immigration as threatening, particularly for the individuals who are high in RWA aggression. We evaluate and find support for this claim using a large-scale survey performed in Sweden (N = 898). The results, showing a conditional effect of immigration attitudes on affective polarization, are consistent across three commonly used measures of affective polarization as follows: trait ratings, a social distance measure, and feeling thermometers. Overall, our results show that it is important to consider intergroup threats and intergroup differences in the context of sensitivity to such threats when explaining affective polarization.
Assessing Emotions of Teaching Assistants in Inclusive Education
Authors: Yang Lan , Hsu Chia-Ling , Ye Tianfang , Sin Kuen Fung
Published: July 2022

Although there are an increasing number of studies on assessing teacher emotions in mainstream education, there is a lack of appropriate measurement tools to evaluate the emotions of teaching assistants (TAs) who need to take care of students with a range of special educational needs (SEN). This study tested the generalizability of the 24-item teacher emotion inventory (TEI), among 204 TAs from 122 secondary schools with inclusive education in Hong Kong. We conducted both confirmatory factor analysis (CFA) and Rasch analysis to test the within-network validity of the TEI. For the between-network validity, we examined the relationships between TAs’ emotions and their attitude toward inclusive education. The Rasch analysis supported the scale’s dimensionality and item fit statistics. The CFA supported the five-factor solution of the TEI. The results also showed statistically significant correlations between positive emotions (joy and love) and TAs’ attitudes toward inclusive education. TAs’ negative emotions (anxiety, anger, and stress) appeared to be negatively correlated with their attitude toward inclusive education. The results supported that TEI is a useful tool to assess the emotions of TAs that play a pivotal role in assisting both school teachers and SEN students, who are more likely to face increased emotional challenges than those not needing to educate SEN students. Implications of this study to enrich the current scope of research on understanding teacher emotions across educational levels and settings are discussed.
Child Homicide Amounting to Murder in Malaysia: Descriptive Analysis of the Statistics and Causes
Authors: Razali Salmi , Jaris Nor Hidayah , Muuti Muhamad Zaid , Abdullah Nuraisyah Chua
Published: July 2022

Objective

To describe the child homicide rates and examine the circumstances of homicides of children in Malaysia from January 2010 to June 2021.

Methods

This is a retrospective secondary analysis of police records on child homicides in Malaysia. Background demographic characteristics of victims, suspected offenders, motives of homicide, and rates of child homicide in Malaysia were analyzed descriptively.

Results

Three hundred thirty-two child homicide cases involving 349 children aged 18 years old and below by 458 suspected offenders were described. The Malaysian rates of child homicide from 2010 to 2018 fluctuated between 0.28 and 0.37 per 100,000 population of children aged 18 and below. The rates show decreasing trend to 0.19 and 0.17 per 100,000 population of children aged 18 in 2019 and 2020, respectively. Most of the victims were Malay and Indians, boys, aged 0–5 years old and 16–18 years old. Offenders were likely to be someone known to the victims, including parents, stepparents, and friends, and have unstable incomes. The main possible motives of homicide are jealousy and revenge, child abuse, and feeling distress.

Conclusion

The rates of child homicide in Malaysia are lower compared to other countries and show decreasing trend during this COVID-19 pandemic. These findings perhaps are underestimated and should be cautiously interpreted. Nevertheless, the results should inform immediate intervention to target the at-risk groups.
Pathways to Positive Youth Development in Malaysian Undergraduate Co-curricular Programs: A Moderated Mediation Model of Youth Voice and Psychological Hardiness
Authors: Nouri Katayoun Mehdinezhad , Krauss Steven E. , Ahrari Seyedali , Ismail Ismi Arif , Arshad Mohd Mursyid
Published: July 2022

Youth voice is gaining more attention globally as a core feature of program quality within positive youth development programs. Few studies have examined the relationship between youth voice and positive youth development in high power-distance cultures, however, where young people often face psychological barriers to exercising decision-making in their work with program adults. Research is needed on the psychological mechanisms that might help youth thrive within settings that are less structurally and psychologically supportive of youth voice. Drawing on bioecological systems and hardiness theories, this quantitative correlational study evaluates the moderating effect of psychological hardiness on the relationship between youth voice, the mediators of program safety and engagement, and the 6 C’s of positive youth development. A moderated mediation model was tested among 436 first-year undergraduate co-curricular program participants from public universities in Malaysia (M = 21.192 years, SD = 1.191 years; 65.6% female). Youth voice positively predicted positive youth development; the relationship was partially mediated by program engagement, but not safety. The mediated pathway through program engagement was more predictive for hardier youth. By combining programmatic and individual psychological factors into the hypothesized model, this research identifies the potential importance of hardiness on the practice of youth voice for young adults in high power distance cultures. The findings highlight the need to identify other individual and programmatic factors that may contribute to the development of positive youth development in diverse cultural settings.
Uyghur–Chinese Adult Bilinguals’ Construal of Voluntary Motion Events
Authors: Tusun Alimujiang
Published: July 2022

The study examined the implications of Talmy motion event typology and Slobin’s thinking-for-speaking hypothesis for the context of Uyghur–Chinese early successive bilingualism. Uyghur and Chinese represent genetically distant languages (Turkic vs. Sino-Tibetan) that nonetheless share important framing properties in the motion domain, i.e., verb-framing. This study thus aimed to establish how this structural overlap would inform bilingual speakers’ construal of motion events. Additionally, it sought to offer an “end state” perspective to a previous study on Uyghur–Chinese child bilinguals and to shed light on issues around the longevity of crosslinguistic influence. Thirty adult Uyghur–Chinese early successive bilinguals were invited to describe a set of voluntary motion events (e.g., “a man runs across the road”). Their verbalizations, alongside those from 24 monolingual Uyghur and 12 monolingual Chinese speakers were systematically analyzed with regard to the kind of linguistic devices used to encode key components of motion (main verb vs. other devices), the frequency with which the components are expressed together (Manner + Path) or separately (Path or Manner) and how they are syntactically packaged. The findings show that the bilinguals’ thinking-for-speaking patterns are largely language-specific, with little crosslinguistic influence. A comparison of our findings with previous studies on Uyghur-Chinese child bilinguals revealed no developmental change either in the analyzed aspects of motion descriptions or in patterns of crosslinguistic influence. As such, the findings lend support to accounts that propose crosslinguistic influence to be a developmental phenomenon.
Age Differences in the Interpretation of Facial Emojis: Classification on the Arousal-Valence Space
Authors: Kutsuzawa Gaku , Umemura Hiroyuki , Eto Koichiro , Kobayashi Yoshiyuki
Published: July 2022

Emojis are universal tools that are frequently used to express people’s emotional states throughout daily communications. They are often applied in various fields of research, such as consumer surveys, as indicators of users’ emotional states. Further analyses of emoji interpretation among people with age are required to ensure the validity of emojis as a metric in such fields of research, thereby reducing misunderstandings. However, details regarding the effect of age on both arousal and valence, as they pertain to the interpretation of emojis, remain unclear. Therefore, in this study, we investigate the effects of the interpretation of facial emojis on the arousal-valence space among people of varying age groups. We conducted an online survey involving 2,000 participants, whereby we employed a nine-point scale to evaluate the valence and arousal levels associated with 74 facial emojis. Based on the two axes of valence and arousal among the age groups involved in this study, emojis are categorized into six similar clusters. For the two negative clusters, i.e., strongly negative and moderately negative sentiments, the group involving middle-aged participants showed significantly higher levels of arousal compared to the group involving young participants. Additionally, not all emojis classified into the aforementioned negative clusters indicate age difference. Based on these results, this study recommends using emojis with no age-related effects on the negative clusters as indices for evaluating human emotions.
Healthcare Worker Mental Health and Wellbeing During COVID-19: Mid-Pandemic Survey Results
Authors: Young Kevin P. , Kolcz Diana L. , Ferrand Jennifer , O’Sullivan David M. , Robinson Kenneth
Published: July 2022

Introduction/Background

HealthCare worker (HCW) mental health and wellbeing are uniquely affected by the complexities of COVID-19 due to exposure to the virus, isolation from family and friends, risk and uncertainty. Little if any inquiry has examined the effects on an entire healthcare system, particularly immediately post-surge. We sought to examine the prevalence of psychiatric symptoms and behavioral health difficulties as a healthcare system transitioned out of the first wave. We assessed the effects of work role, setting and individual diversity factors on employee distress and coping strategies.

Materials and Methods

This was an Institutional Review Board approved, unfunded, voluntary survey sent via REDCap link, to all employees of Hartford HealthCare, a mid-sized healthcare system (N ≈ 29,900) between May 15th and June 26th, 2020. Two system-wide emails and two emails targeting managers were sent during this time frame. Eight thousand four hundred and ninety four employees (28.4% of all e-mails distributed) participated in the survey, representing clinical, support, administrative, and medical staff across hospital, outpatient, residential, and business settings. The survey contained items assessing personal background, work environment/culture, and formal measures, including: patient health questionnaire-9 (PHQ-9), general anxiety disorder-7 (GAD-7), primary care post-traumatic stress disorder screen for DSM-5 (PC-PTSD), alcohol use disorders identification test (AUDIT-C), and the insomnia severity index (ISI).

Results

Almost 1/3 of respondents (31%) reported symptoms of clinically significant anxiety; 83% moderate to severe depression; and 51% moderate to severe insomnia. Thirteen percent screened positive for post-traumatic stress disorder. Frontline staff (p ≤ 0.001 vs. others) and females (p < 0.001 vs. males) endorsed the highest levels of distress, while race (p ≤ 0.005) and ethnicity (p < 0.03 for anxiety, PTSD and insomnia) had a complex and nuanced interaction with symptoms.

Conclusion

Pandemic stress effects all healthcare employees, though not equally. The effects of work role and environment are intuitive though critical. These data suggest individual diversity factors also play an important role in mental health and wellbeing. All must be considered to optimize employee functioning.
Temporal Relationships Between Abdominal Pain, Psychological Distress and Coping in Patients With IBS – A Time Series Approach
Authors: Engel Felicitas , Stadnitski Tatjana , Stroe-Kunold Esther , Berens Sabrina , Schäfert Rainer , Wild Beate
Published: July 2022

Objective

Irritable bowel syndrome (IBS) is a chronic disease leading to abdominal pain that is often related to psychological distress. The aim of the study was to investigate the temporal relationships between abdominal pain and psychological variables in patients with IBS.

Methods

This longitudinal diary study included eight patients from a waiting group, recruited in the frame of a pilot intervention study. During their waiting time of 3 months the patients answered questions daily regarding somatic and psychological variables using an online diary. All patients were considered and analyzed as single cases. The temporal dynamics between the time series of psycho-somatic variables were analyzed using a vector autoregressive (VAR) modeling approach.

Results

For all patients, positive same-day correlations between somatic and psychological time series were observed. The highest same-day correlations were found between somatic symptoms and pain-related discomfort (r = 0.40 to r = 0.94). Altogether, n = 26 significant lagged relationships were identified; n = 17 (65%) indicated that somatic values were predictive of psychological complaints on the following days. N = 9 (35%) lagged relationships indicated an opposite relationship in that psychological complaints were predictive of somatic symptoms. Three patients showed a significant positive same-day correlation between abdominal pain and use of a positive coping strategy. However, significant lagged relationships in two patients showed that for these patients the use of positive thinking as a coping strategy was unhelpful in reducing pain on the following days.

Conclusions

In patients with IBS abdominal symptoms appear to be closely related to psychological symptoms. For some patients, somatic complaints predict psychological complaints, in other patients the directionality is opposite. The impact of coping strategies on somatic symptoms varies among patients, therefore their role for a possible reduction of pain should be further explored. The results suggest the need of characterizing patientsindividually for effective health interventions. Individual time series analyses provide helpful tools for finding reasonable person-level moderators.
A Qualitative Study of Self and Caregiver Perspectives on How Autistic Individuals Cope With Trauma
Authors: Ng-Cordell Elise , Rai Anika , Peracha Hira , Garfield Tamara , Lankenau Stephen E. , Robins Diana L. , Berkowitz Steven J. , Newschaffer Craig , Kerns Connor M.
Published: July 2022

Background

Coping can moderate the relationship between trauma exposure and trauma symptoms. There are many conceptualisations of coping in the general population, but limited research has considered how autistic individuals cope, despite their above-average rates of traumatic exposure.

Objectives

To describe the range of coping strategies autistic individuals use following traumatic events.

Methods

Fourteen autistic adults and 15 caregivers of autistic individuals, recruited via stratified purposive sampling, completed semi-structured interviews. Participants were asked to describe how they/their child attempted to cope with events they perceived as traumatic. Using an existing theoretical framework and reflexive thematic analysis, coping strategies were identified, described, and organized into themes.

Results

Coping strategies used by autistic individuals could be organized into 3 main themes: (1) Engaging with Trauma, (2) Disengaging from Trauma, and (3) Self-Regulatory Coping. After the three main themes were developed, a fourth integrative theme, Diagnostic Overshadowing, was created to capture participants' reports of the overlap or confusion between coping and autism-related behaviors.

Conclusions

Autistic individuals use many strategies to cope with trauma, many of which are traditionally recognized as coping, but some of which may be less easily recognized given their overlap with autism-related behaviors. Findings highlight considerations for conceptualizing coping in autism, including factors influencing how individuals cope with trauma, and how aspects of autism may shape or overlap with coping behavior. Research building on these findings may inform a more nuanced understanding of how autistic people respond to adversity, and how to support coping strategies that promote recovery from trauma.
Inducible Nitric Oxide Synthase Inhibition in the Medial Prefrontal Cortex Attenuates the Anxiogenic-Like Effect of Acute Restraint Stress via CB1 Receptors
Authors: Coelho Arthur A. , Vila-Verde Carla , Sartim Ariandra G. , Uliana Daniela L. , Braga Laura A. , Guimarães Francisco S. , Lisboa Sabrina F.
Published: July 2022

Stress exposure can result in several proinflammatory alterations in the brain, including overexpression of the inducible isoform of nitric oxide synthase (iNOS) in the medial prefrontal cortex (mPFC). These changes may be involved in the development of many psychiatric conditions. However, it is unknown if iNOS in mPFC plays a significant role in stress-induced behavioral changes. The endocannabinoid (ECB) system is also influenced by stress. Its activation seems to be a counter regulatory mechanism to prevent or decrease the stress-mediated neuroinflammatory consequences. However, it is unclear if the ECB system and iNOS interact to influence stress consequences. This study aimed to test the hypothesis that the anti-stress effect of iNOS inhibition in mPFC involves the local ECB system, particularly the CB1 cannabinoid receptors. Male Wistar rats with guide cannula aimed at the mPFC were submitted to acute restraint stress (RS) for 2 h. In the following morning, rats received bilateral microinjections of vehicle, AM251 (CB1 antagonist; 100 pmol), and/or 1400W (iNOS selective inhibitor; 10−4, 10−3, or 10−2 nmol) into the prelimbic area of mPFC (PL-mPFC) before being tested in the elevated plus-maze (EPM). iNOS inhibition by 1400W prevented the anxiogenic-like effect observed in animals submitted to RS. The drug did not promote behavior changes in naive animals, demonstrating a stress-dependent effect. The 1400W-anti-stress effect was prevented by local pretreatment with AM251. Our data suggest that iNOS inhibition may facilitate the local endocannabinoid signaling, attenuating stress effects.
The Utility of Cognitive Screening in Asian Patients With Heart Failure: A Systematic Review
Authors: Niu Qi , Liu WeiHua , Wang FengLing , Tian LiYa , Dong YanHong
Published: July 2022

Background

The prevalence of undiagnosed cognitive impairment in patients with heart failure is alarmingly high in Asia. There is still no consensus on cognitive screening tools to detect cognitive impairment in the Asian heart failure population. The clinical implications based on our systematic review may help to improve cognitive screening practice for patients with heart failure in Asia.

Methods

This review is registered in the PROSPERO (CRD42021264288). Using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) approach, we searched PubMed, Embase, the Cumulative Index to Nursing and Allied Health Literature, Scopus, the Web of Science, PsycINFO, the Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, and Wanfang Data in English and Chinese literatures concerning heart failure and cognitive impairment.

Results

The search yielded 21 eligible studies. Only in five studies, cognitive brief tests, including the Montreal Cognitive Assessment (MoCA), the Mini-Mental State Examination (MMSE), and the Mini-Cog, were used as cognitive screening tools for Asian patients with heart failure. In the rest 16 studies, brief cognitive tests were used as screening tools for global cognition. Only one study validated screening tests against a gold standard formal neuropsychological assessment test battery. Among these studies, patients with heart failure tended to perform worse than patients without heart failure. The presence of cognitive impairment in patients with heart failure is associated with poorer self-care, quality of life, and hospital readmission.

Conclusion

Brief cognitive tests have been used in Asian patients with heart failure and these tests are frequently used as a measure of global cognitive function for cognitive screening. However, validating brief cognitive tests against a gold standard formal neuropsychological assessment in Asian patients with heart failure is lacking. Future studies need to address methodological issues to validate cognitive screening measures in a larger population of Asian patients with heart failure.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/
Role of an Online Skill-Based Mindfulness Program for Healthcare Worker's Resiliency During the COVID-19 Pandemic: A Mixed-Method Study
Authors: Kim Soyeon , Crawford Jennifer , Hunter Sarah
Published: July 2022

The COVID-19 pandemic has highlighted the mental health care needs of health care workers. The primary aim of this study was to explore the effects of an online mindfulness program on resiliency in health care workers during the pandemic using a mixed-methods approach. An online 4-week mindfulness program was delivered to healthcare workers (N = 130) in Ontario, Canada. Resiliency was assessed at 3-time points (i.e., pre, post, and 1-month follow-up), and the mindfulness program's efficacy on resiliency was analyzed using linear regression. Semi-structured interviews (N = 10) were conducted to substantiate their experiences. Healthcare workers' resiliency significantly increased after the mindfulness program compared to the baseline, maintaining the effect after 1 month in both unadjusted and adjusted regression models. These findings were further bolstered by the positive experiences shared by participants highlighting the program's efficacy on empathy and resiliency. Evidence suggests that mindfulness is associated with promoting resiliency in healthcare workers and may be an important strategy to promote resiliency in this population.
Optimization of Multi-Objective Mobile Emergency Material Allocation for Sudden Disasters
Authors: Li Jianxun , Fu Haoxin , Lai Kin Keung , Ram Bhagwat
Published: July 2022

The mobile emergency system is a new emergency mode that provides a solution to deal with increasingly frequent sudden disasters by reasonably allocating mobile emergency facilities and optimizing the allocation of mobile emergency materials. We consider mobile emergency cost and mobile emergency time as two objective functions. This paper establishes a multi-objective mobile emergency material allocation model, and transforms the multi-objective. We choose the emergency material transportation path for coding, and apply the hybrid leapfrog algorithm for material allocation to obtain the optimal solution. Finally, the feasibility of the model is verified by taking Zhengzhou urban area under the “21.7” severe rainstorm and flood disaster in Henan Province. The result analyses show that the model can correspond to each stage of mobile emergency material allocation based on the value of cost preference, and put forward suggestions on the location of mobile emergency facilities and the amount of material allocation.
Non-linear Relationship of Maternal Age With Risk of Spontaneous Abortion: A Case-Control Study in the China Birth Cohort
Authors: Zhang Man , Yang Bo-Yi , Sun Yongqing , Qian Zhengmin , Xaverius Pamela K. , Aaron Hannah E. , Zhao Xiaoting , Zhang Zheng , Liu Ruixia , Dong Guang-Hui , Yin Chenghong , Yue Wentao
Published: July 2022

Background

Spontaneous abortion is one of the prevalent adverse reproductive outcomes, which seriously threatens maternal health around the world.

Objective

The current study is aimed to evaluate the association between maternal age and risk for spontaneous abortion among pregnant women in China.

Methods

This was a case-control study based on the China Birth Cohort, we compared 338 cases ending in spontaneous abortion with 1,352 controls resulting in normal live births. The main exposure indicator and outcome indicator were maternal age and spontaneous abortion, respectively. We used both a generalized additive model and a two-piece-wise linear model to determine the association. We further performed stratified analyses to test the robustness of the association between maternal age and spontaneous abortion in different subgroups.

Results

We observed a J-shaped relationship between maternal age and spontaneous abortion risk, after adjusting for multiple covariates. Further, we found that the optimal threshold age was 29.68 years old. The adjusted odds ratio (95% confidence interval) of spontaneous abortion per 1 year increase in maternal age were 0.97 (0.90–1.06) on the left side of the turning point and 1.25 (1.28–1.31) on the right side. Additionally, none of the covariates studied modified the association between maternal age and spontaneous abortion (P > 0.05).

Conclusions

Advanced maternal age (>30 years old) was significantly associated with increased prevalence of spontaneous abortion, supporting a J-shaped association between maternal age and spontaneous abortion.