bluuebunny/crossref_metadata_2025 huggingface dataset:
Whose responsibility? Part 1 of 2: A scale to assess how stakeholders apportion responsibilities for addressing the needs of persons with mental health problems
Authors: Iyer Srividya N. , Pope Megan , Taksal Aarati , Mohan Greeshma , Rangaswamy Thara , Loohuis Heleen , Shah Jai , Joober Ridha , Schmitz Norbert , Margolese Howard C. , Padmavati Ramachandran , Malla Ashok
Published: January 2022

Background

Individuals with mental health problems have multiple, often inadequately met needs. Responsibility for meeting these needs frequently falls to patients, their families/caregivers, and governments. Little is known about stakeholders' views of who should be responsible for these needs and there are no measures to assess this construct. This study’s objectives were to present the newly designed Whose Responsibility Scale (WRS), which assesses how stakeholders apportion responsibility to persons with mental health problems, their families, and the government for addressing various needs of persons with mental health problems, and to report its psychometric properties.

Methods

The 22-item WRS asks respondents to assign relative responsibility to the government versus persons with mental health problems, government versus families, and families versus persons with mental health problems for seven support needs. The items were modelled on a World Values Survey item comparing the government’s and people’s responsibility for ensuring that everyone is provided for. We administered English, Tamil, and French versions to 57 patients, 60 family members, and 27 clinicians at two early psychosis programs in Chennai, India, and Montreal, Canada, evaluating test–retest reliability, internal consistency, and ease of use. Internal consistency estimates were also calculated for confirmatory purposes with the larger samples from the main comparative study.

Results

Test–retest reliability (intra-class correlation coefficients) generally ranged from excellent to fair across stakeholders (patients, families, and clinicians), settings (Montreal and Chennai), and languages (English, French, and Tamil). In the standardization and larger confirmatory samples, internal consistency estimates (Cronbach’s alphas) ranged from acceptable to excellent. The WRS scored average on ease of comprehension and completion. Scores were spread across the 1–10 range, suggesting that the scale captured variations in views on how responsibility for meeting needs should be distributed. On select items, scores at one end of the scale were never endorsed, but these reflected expected views about specific needs (e.g., Chennai patients never endorsed patients as being substantially more responsible for housing needs than families).

Conclusions

The WRS is a promising measure for use across geo-cultural contexts to inform mental health policies, and to foster dialogue and accountability among stakeholders about roles and responsibilities. It can help researchers study stakeholders’ views about responsibilities, and how these shape and are shaped by sociocultural contexts and mental healthcare systems.
Knockout of circRNAs by base editing back-splice sites of circularized exons
Authors: Gao Xiang , Ma Xu-Kai , Li Xiang , Li Guo-Wei , Liu Chu-Xiao , Zhang Jun , Wang Ying , Wei Jia , Chen Jia , Chen Ling-Ling , Yang Li
Published: January 2022

Many circular RNAs (circRNAs) are produced from back-splicing of exons of precursor mRNAs and are generally co-expressed with cognate linear RNAs. Methods for circRNA-specific knockout are lacking, largely due to sequence overlaps between forms. Here, we use base editors (BEs) for circRNA depletion. By targeting splice sites involved in both back-splicing and canonical splicing, BEs can repress circular and linear RNAs. Targeting sites predominantly for circRNA biogenesis, BEs could efficiently repress the production of circular but not linear RNAs. As hundreds of exons are predominantly back-spliced to produce circRNAs, this provides an efficient method to deplete circRNAs for functional study.
Evolution of the repression mechanisms in circadian clocks
Authors: Tyler Jonathan , Lu Yining , Dunlap Jay , Forger Daniel B.
Published: January 2022

Background

Circadian (daily) timekeeping is essential to the survival of many organisms. An integral part of all circadian timekeeping systems is negative feedback between an activator and repressor. However, the role of this feedback varies widely between lower and higher organisms.

Results

Here, we study repression mechanisms in the cyanobacterial and eukaryotic clocks through mathematical modeling and systems analysis. We find a common mathematical model that describes the mechanism by which organisms generate rhythms; however, transcription’s role in this has diverged. In cyanobacteria, protein sequestration and phosphorylation generate and regulate rhythms while transcription regulation keeps proteins in proper stoichiometric balance. Based on recent experimental work, we propose a repressor phospholock mechanism that models the negative feedback through transcription in clocks of higher organisms. Interestingly, this model, when coupled with activator phosphorylation, allows for oscillations over a wide range of protein stoichiometries, thereby reconciling the negative feedback mechanism in Neurospora with that in mammals and cyanobacteria.

Conclusions

Taken together, these results paint a picture of how circadian timekeeping may have evolved.
Phiclust: a clusterability measure for single-cell transcriptomics reveals phenotypic subpopulations
Authors: Mircea Maria , Hochane Mazène , Fan Xueying , Chuva de Sousa Lopes Susana M. , Garlaschelli Diego , Semrau Stefan
Published: January 2022

The ability to discover new cell phenotypes by unsupervised clustering of single-cell transcriptomes has revolutionized biology. Currently, there is no principled way to decide whether a cluster of cells contains meaningful subpopulations that should be further resolved. Here, we present phiclust (ϕclust), a clusterability measure derived from random matrix theory that can be used to identify cell clusters with non-random substructure, testably leading to the discovery of previously overlooked phenotypes.
Ivermectin as an endectocide may boost control of malaria vectors in India and contribute to elimination
Authors: Ahmad Sundus Shafat , Rahi Manju , Saroha Poonam , Sharma Amit
Published: January 2022

Malaria constitutes one of the largest public health burdens faced by humanity. Malaria control has to be an efficient balance between diagnosis, treatment and vector control strategies. The World Health Organization currently recommends indoor residual spraying and impregnated bed nets as two malaria vector control methods that have shown robust and persistent results against endophilic and anthropophilic mosquito species. The Indian government launched the National Framework for Malaria Elimination in 2016 with the aim to achieve the elimination of malaria in a phased and strategic manner and to sustain a nation-wide malaria-free status by 2030. India is currently in a crucial phase of malaria elimination and novel vector control strategies maybe helpful in dealing with various challenges, such as vector behavioural adaptations and increasing insecticide resistance among the Anopheles populations of India. Ivermectin can be one such new tool as it is the first endectocide to be approved in both animals and humans. Trials of ivermectin have been conducted in endemic areas of Africa with promising results. In this review, we assess available data on ivermectin as an endectocide and propose that this endectocide should be explored as a vector control tool for malaria in India.

Graphical Abstract
A dataset for dental anxiety and psychological distress in 1550 patients visiting dental clinics
Authors: Zinke Alexander , Bohl Christin , Berth Hendrik
Published: January 2022

Objectives

Information was collected to identify anxiety in dental patients visiting a dental clinic using the Dental Anxiety Scale, their level of psychological distress using the Brief Symptom Inventory-18 and identifying a correlation between these groups as well as the gender and age.

Data description

This data contains a set of 1550 patients’ answers to questionnaires taken before dental treatment in a dental clinic. It is divided into male and female patients as well as according to their age. The level of Dental Anxiety can be interpreted by answers chosen in the Dental Anxiety Scale (DAS) and the level of psychological distress by answers chosen in the Brief Symptom Inventory-18 (BSI-18). This dataset should help to encourage more research in the field of dental anxiety and we hope to see more comparisons with our data in the future or in different regions of the world.
CRISPR/Cas9 genome editing system confirms centriolin’s role in cytokinesis
Authors: Seronick Eric , Son Jae , Michael Cameron , Fogg Hannah , Gromley Zeynep , Gromley Adam
Published: January 2022

Objective

In addition to its function as the microtubule organizing center of the cell, the centrosome has functions in many other cellular processes including primary cilia formation, DNA damage checkpoints, and cell cycle progression. But the role of individual components of the centrosome in these processes remains unclear. Previous studies used siRNA (small interfering RNA) to “knock down” protein levels of the centrosome component centriolin, resulting in failed cytokinesis. Since this approach was transient, only targeting centriolin at the mRNA level, we sought to confirm these findings by permanently disrupting the gene encoding centriolin using the CRISPR/Cas9 system of genome editing.

Results

This study provides evidence that the CRISPR/Cas9 system is capable of effectively reducing centriolin protein levels in the cell. Furthermore, this disruption leads to a failure of cytokinesis that is reminiscent of the phenotype previously reported for the siRNA-mediated disruption of centriolin. Furthermore, no additional defects in cell division were observed, consistent with results seen with previous siRNA studies. We conclude that the CRISPR/Cas9 system is an effective means of permanently removing the cellular pools of centriolin and that the disruption of centriolin at both the mRNA level and genomic level lead to similar cell division defects.
Using parallel pre-trained types of DCNN model to predict breast cancer with color normalization
Authors: Al Noumah William , Jafar Assef , Al Joumaa Kadan
Published: January 2022

Objective

Breast cancer is the most common among women, and it causes many deaths every year. Early diagnosis increases the chance of cure through treatment. The traditional manual diagnosis requires effort and time from pathological experts, as it needs a joint experience of a number of pathologists. Diagnostic mistakes can lead to catastrophic results and endanger the lives of patients. The presence of an expert system that is able to specify whether the examined tissue is healthy or not, thus improves the quality of diagnosis and saves the time of experts. In this paper, a model capable of classifying breast cancer anatomy by making use of a pre-trained DCNN has been proposed. To build this model, first of all the image should be color stained by using Vahadane algorithm, then the model which combines three pre-trained DCNN (Xception, NASNet and Inceptoin_Resnet_V2) should be built in parallel, then the three branches should be aggregated to take advantage of each other. The suggested model was tested under different values of threshold ratios and also compared with other models.

Results

The proposed model on the BreaKHis dataset achieved 98% accuracy, which is better than the accuracy of other models used in this field.
Artificial intelligence framework identifies candidate targets for drug repurposing in Alzheimer’s disease
Authors: Fang Jiansong , Zhang Pengyue , Wang Quan , Chiang Chien-Wei , Zhou Yadi , Hou Yuan , Xu Jielin , Chen Rui , Zhang Bin , Lewis Stephen J. , Leverenz James B. , Pieper Andrew A. , Li Bingshan , Li Lang , Cummings Jeffrey , Cheng Feixiong
Published: January 2022

Background

Genome-wide association studies (GWAS) have identified numerous susceptibility loci for Alzheimer’s disease (AD). However, utilizing GWAS and multi-omics data to identify high-confidence AD risk genes (ARGs) and druggable targets that can guide development of new therapeutics for patients suffering from AD has heretofore not been successful.

Methods

To address this critical problem in the field, we have developed a network-based artificial intelligence framework that is capable of integrating multi-omics data along with human protein–protein interactome networks to accurately infer accurate drug targets impacted by GWAS-identified variants to identify new therapeutics. When applied to AD, this approach integrates GWAS findings, multi-omics data from brain samples of AD patients and AD transgenic animal models, drug-target networks, and the human protein–protein interactome, along with large-scale patient database validation and in vitro mechanistic observations in human microglia cells.

Results

Through this approach, we identified 103 ARGs validated by various levels of pathobiological evidence in AD. Via network-based prediction and population-based validation, we then showed that three drugs (pioglitazone, febuxostat, and atenolol) are significantly associated with decreased risk of AD compared with matched control populations. Pioglitazone usage is significantly associated with decreased risk of AD (hazard ratio (HR) = 0.916, 95% confidence interval [CI] 0.861–0.974, P = 0.005) in a retrospective case-control validation. Pioglitazone is a peroxisome proliferator-activated receptor (PPAR) agonist used to treat type 2 diabetes, and propensity score matching cohort studies confirmed its association with reduced risk of AD in comparison to glipizide (HR = 0.921, 95% CI 0.862–0.984, P = 0.0159), an insulin secretagogue that is also used to treat type 2 diabetes. In vitro experiments showed that pioglitazone downregulated glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase (CDK5) in human microglia cells, supporting a possible mechanism-of-action for its beneficial effect in AD.

Conclusions

In summary, we present an integrated, network-based artificial intelligence methodology to rapidly translate GWAS findings and multi-omics data to genotype-informed therapeutic discovery in AD.
Puromycin-based purification of cells with high expression of the cytochrome P450 CYP3A4 gene from a patient with drug-induced liver injury (DILI)
Authors: Miyata Shoko , Saku Noriaki , Akiyama Saeko , Javaregowda Palaksha Kanive , Ite Kenta , Takashima Nagisa , Toyoda Masashi , Yura Kei , Kimura Tohru , Nishina Hiroshi , Nakazawa Atsuko , Kasahara Mureo , Nonaka Hidenori , Kiyono Tohru , Umezawa Akihiro
Published: January 2022

Background

Many drugs have the potential to induce the expression of drug-metabolizing enzymes, particularly cytochrome P450 3A4 (CYP3A4), in hepatocytes. Hepatocytes can be accurately evaluated for drug-mediated CYP3A4 induction; this is the gold standard for in vitro hepatic toxicology testing. However, the variation from lot to lot is an issue that needs to be addressed. Only a limited number of immortalized hepatocyte cell lines have been reported. In this study, immortalized cells expressing CYP3A4 were generated from a patient with drug-induced liver injury (DILI).

Methods

To generate DILI-derived cells with high expression of CYP3A4, a three-step approach was employed: (1) Differentiation of DILI-induced pluripotent stem cells (DILI-iPSCs); (2) Immortalization of the differentiated cells; (3) Selection of the cells by puromycin. It was hypothesized that cells with high cytochrome P450 gene expression would be able to survive exposure to cytotoxic antibiotics because of their increased drug-metabolizing activity. Puromycin, a cytotoxic antibiotic, was used in this study because of its rapid cytocidal effect at low concentrations.

Results

The hepatocyte-like cells differentiated from DILI-iPSCs were purified by exposure to puromycin. The puromycin-selected cells (HepaSM or SI cells) constitutively expressed the CYP3A4 gene at extremely high levels and exhibited hepatocytic features over time. However, unlike primary hepatocytes, the established cells did not produce bile or accumulate glycogen.

Conclusions

iPSC-derived hepatocyte-like cells with intrinsic drug-metabolizing enzymes can be purified from non-hepatocytes and undifferentiated iPSCs using the cytocidal antibiotic puromycin. The puromycin-selected hepatocyte-like cells exhibited characteristics of hepatocytes after immortalization and may serve as another useful source for in vitro hepatotoxicity testing of low molecular weight drugs.
Update of application of olfactory ensheathing cells and stem cells/exosomes in the treatment of retinal disorders
Authors: Yu Yang , Li Licheng , Lin Shu , Hu Jianmin
Published: January 2022

Age-related macular degeneration, diabetic retinopathy, retinitis pigmentosa and other retinal disorders are the main causes of visual impairment worldwide. In the past, these retinal diseases, especially dry age-related macular degeneration, proliferative diabetic retinopathy and retinitis pigmentosa, were treated with traditional surgery and drugs. However, the effect was moderate. In recent years, researchers have used embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells, olfactory ensheathing cells and other stem cells to conduct experiments and found that stem cells can inhibit inflammation, regulate immune response, secrete neurotrophic factors, and differentiate into retinal cells to replace and promote restoration of the damaged parts. These stem cells have the potential to treat retinal diseases. Whether it is in animal experiments or clinical trials, the increase in the number of retinal cells, maintenance of function and improvement of visual function all reflect the advanced of stem cells to treat retinal diseases, but its risk preserves the donor’s hidden pathogenic genes, immune rejection and tumorigenicity. With the development of exosomes study, researchers have discovered that exosomes come from a wide range of sources and can be secreted by almost all types of cells. Using exosomes with stem cell to treat retinal diseases is more effective than using stem cells alone. This review article summarizes the recent advances in the application of olfactory ensheathing cells and stem cells/exosomes in the treatment of retinal disorders.
Mesenchymal stromal cells attenuate alveolar type 2 cells senescence through regulating NAMPT-mediated NAD metabolism
Authors: Lai Xiaofan , Huang Shaojie , Lin Sijia , Pu Lvya , Wang Yaqing , Lin Yingying , Huang Wenqi , Wang Zhongxing
Published: January 2022

Background

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive deadly fibrotic lung disease with high prevalence and mortality worldwide. The therapeutic potential of mesenchymal stem cells (MSCs) in pulmonary fibrosis may be attributed to the strong paracrine, anti-inflammatory, anti-apoptosis and immunoregulatory effects. However, the mechanisms underlying the therapeutic effects of MSCs in IPF, especially in terms of alveolar type 2 (AT2) cells senescence, are not well understood. The purpose of this study was to evaluate the role of MSCs in NAD metabolism and senescence of AT2 cells in vitro and in vivo.

Methods

MSCs were isolated from human bone marrow. The protective effects of MSCs injection in pulmonary fibrosis were assessed via bleomycin mouse models. The senescence of AT2 cells co-cultured with MSCs was evaluated by SA-β-galactosidase assay, immunofluorescence staining and Western blotting. NAD+ level and NAMPT expression in AT2 cells affected by MSCs were determined in vitro and in vivo. FK866 and NAMPT shRNA vectors were used to determine the role of NAMPT in MSCs inhibiting AT2 cells senescence.

Results

We proved that MSCs attenuate bleomycin-induced pulmonary fibrosis in mice. Senescence of AT2 cells was alleviated in MSCs-treated pulmonary fibrosis mice and when co-cultured with MSCs in vitro. Mechanistic studies showed that NAD+ and NAMPT levels were rescued in AT2 cells co-cultured with MSCs and MSCs could suppress AT2 cells senescence mainly via suppressing lysosome-mediated NAMPT degradation.

Conclusions

MSCs attenuate AT2 cells senescence by upregulating NAMPT expression and NAD+ levels, thus exerting protective effects in pulmonary fibrosis.
Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.
Authors: Ahmadpoor Fatemeh , Zare Nasser , Asghari Rasool , Sheikhzadeh Parisa
Published: January 2022

Melia azedarach L. is a valuable source of antioxidants and secondary metabolites. This study is a first extensive report about the effect of different serialization protocols and plant growth regulators (PGRs) on explant disinfection efficiency, callus induction and secondary metabolites production and accumulation in callus cultures of M. azedarach L. In this regard, the effect of plant growth regulators on callus induction and secondary metabolites production were examined. In addition, different sterilization agents were evaluated for disinfection of chinaberry leaf explants. The results showed that the lowest percentage of explant contamination and browning with the highest percentage of callus induction and callus growth obtained with explants pretreated with benomyl (2 g/L) for 2 h and sterilized with 7% H2O2 for 10 min and NaOCl 2% (without pH adjustment) for 12 min. Although adjusting the pH of NaOCl to pH  = 7 and 10 significantly reduced the microbial contamination and increased the percentage of contamination-free cultures of M. azedarach L., adversely influenced the explant viability and callus induction and growth. The highest percentage of callus induction obtained on the MS medium containing 3 mg/L NAA/2,4-D and 1 or 3 mg/L Kin/BAP, and the highest callus yield (1804.833 mg/explant) belonged to the MS medium supplemented with 5 mg/L 2,4-D and 5 mg/L Kin. The callus cultures grown on the MS medium supplemented with 3 mg/L NAA and 1 mg/L Kin produced the highest amount of Quercetin (2.06 mg/g fresh weight), Rutin (5.56 mg/g fresh weight) and Kaempferol (1.84 mg/g fresh weight).
Open-access database of literature derived drug-related Torsade de Pointes cases
Authors: Krumpholz Laura , Wiśniowska Barbara , Polak Sebastian
Published: January 2022

Since an introduction of an ICH guidance in 2005, no new drugs were withdrawn from the market because of the causation of Torsade de Pointes (TdP). However, the risk of TdP is still a concern for marketed drugs. TdP is a type of polymorphic ventricular tachycardia which may lead to sudden cardiac death. QT/QTc interval prolongation is considered a sensitive, but not specific biomarker. To improve the effectiveness of studies’ workflow related to TdP risk prediction we created an extensive, structured, open-access database of drug-related TdP cases. PubMed, Google Scholar bibliographic databases, and the Internet, via the Google search engine, were searched to identify eligible reports. A total of 424 papers with a description of 634 case reports and observational studies were included. Each paper was manually examined and listed with up to 53 variables related to patient/population characteristics, general health parameters, used drugs, laboratory measurements, ECG results, clinical management, and its outcomes, as well as suspected drug’s properties and its FDA adverse reaction reports. The presented database may be considered as an extension of the recently developed and published database of drug cardiac safety-related information, part of the tox-portal project providing resources for cardiac toxicity assessment.
Predictability of IELTS in a high-stakes context: a mixed methods study of Chinese students’ perspectives on test preparation
Authors: Ma Hui , Chong Sin Wang
Published: January 2022

High-stakes language tests are used around the world as a gatekeeping tool under the internationalization of higher education. However, the predictable aspect of the high-stakes language tests is seldom discussed, especially from students’ perspectives. This study aims to address this gap by aiming to better understand how certain factors and conditions contribute to the predictability issue of IELTS from students’ perspectives within a high-stakes context. This study used a mixed method approach to investigate the views and experiences of students within a Sino-UK joint college. The data collection was in two concurrent strands: online survey and group interviews. Findings suggested that IELTS can impact students negatively by narrowing their English learning scope, driving them into self-isolated way of study, doing repeated test-taking and buying predicted answers. Implications related to language test preparation are discussed in light of the findings.
Quantitative analysis of vitamin D using m/MALDI-TOF mass spectrometry based on a parylene matrix chip
Authors: Noh Joo-Yoon , Kim Moon-Ju , Park Jong-Min , Yun Tae Gyeong , Kang Min-Jung , Pyun Jae-Chul
Published: January 2022

Vitamin D deficiency is associated with various disorders and is diagnosed based on the concentration of 25-hydroxy vitamin D3 (25(OH)D3) in serum. The parylene matrix chip was fabricated to reduce the matrix background noise, and the homogenous distribution of the matrix was retained for the quantitative analysis of 25(OH)D3. The Amplex Red assay was performed to confirm that the sample-matrix mixing zone of the parylene matrix chip was formed below the surface of the parylene-N film. The homogeneous distribution of the matrix was verified from the fluorescence image. For effective analysis using a parylene matrix chip, 25(OH)D3 was modified through the nucleophilic addition of betaine aldehyde (BA) to form a hemiacetal salt. Such modified 25(OH)D3 with a positive charge from BA could be effectively analyzed using MALDI-TOF mass spectrometry. Serum 25(OH)D3 was extracted by liquid–liquid extraction (LLE) and quantified using MALDI-TOF mass spectrometry based on the parylene matrix chip. The intensity of the mass peak of 25(OH)D3 was linearly correlated (r2 = 0.992) with the concentration of 25(OH)D3 spiked in serum, and the LOD was 0.0056 pmol/μL. Energy drinks and vitamin D3 tablets were also employed for the real sample analysis. Finally, the results of the chemiluminescence binding assay and MALDI-TOF mass spectrometry were statistically analyzed to determine the applicability of the method using the Bland–Altman test and Passing–Bablok regression.
Upper crustal shear-wave velocity structure Beneath Western Java, Indonesia from seismic ambient noise tomography
Authors: Rosalia Shindy , Widiyantoro Sri , Cummins Phil R. , Yudistira Tedi , Nugraha Andri Dian , Zulfakriza Zulfakriza , Setiawan Ahmad
Published: January 2022

This paper presents the depth inversion of Rayleigh wave group velocity to obtain an S-wave velocity model from seismic ambient noise cross-correlation in western Java, Indonesia. This study utilizes the vertical component data of a temporary seismograph network deployed in 2016, which was used in a previous study to estimate fundamental mode Rayleigh wave group velocity maps. In this study, the Neighborhood Algorithm was applied to invert the Rayleigh wave group velocities into 1D shear-wave velocity (Vs) profiles, which were then interpolated to produce a high-resolution, pseudo-3D Vs model. These tomographic images of Vs extend to ~ 20 km depth and show a pronounced NE-SW contrast of low and high Vs in the depth range 1–5 km that correlates well with the Bouguer anomaly map. We interpret the low Vs in the northeastern part of the study area as associated with alluvial and volcanic products from the Sunda Shelf and modern volcanic arc, whereas the high Vs in the southwestern part is associated with volcanic arc products from earlier episodes of subduction. We also obtained the depth of the northern Java Basin, which is in the range of 5–6 km, and the Garut Basin, which extends to 5 km depth. For greater depths, Vs gradually increases throughout western Java, which reflects the crystalline basement. This study provides estimates of the shallow crustal Vs structure underneath West Java with higher resolution than previous tomographic studies, which could be useful for supporting future earthquake studies in the region.
Understanding the secondary system of therapeutic alliance in autism interventions from the perspectives of parents and caregivers
Authors: Rilveria John Robert C.
Published: January 2022

This study utilized a sequential explanatory mixed-methods design in understanding the secondary system of therapeutic alliance from the perspectives of parents and caregivers of children with autism. In the quantitative phase, data from the accomplished questionnaires of 124 parent–caregiver dyads were collected and analyzed. Four factors extracted from the literature: treatment attitude, treatment compliance, level of stress, and perceived autism severity were examined as possible predictors of parent–therapist alliance, caregiver–therapist alliance, and parent–caregiver alliance. In the qualitative phase, eight parent–caregiver dyads (who both scored high in their alliance with therapist, alliance with each other, treatment attitude, treatment compliance, and level of stress) were interviewed to probe on their experiences of alliance-building, parenting, and caregiving. The integration of both quantitative and qualitative data led to the formulation of a conceptual framework that explains how the factors influence the secondary system of therapeutic alliance. These findings emphasize that tapping into the personal experiences of parents and caregivers can help in identifying what they value in a working, therapeutic relationship. Moreover, communication, skills training, and feedback among therapists, parents, and caregivers are essential to enhance positive attitude toward treatment, promote compliance to treatment recommendations, and address sources of personal stress. Nevertheless, this study calls for future studies to build into the factors associated with the systemic therapeutic alliance and to implement intervention programs that may target issues relating to attitude toward treatment, compliance to treatment, and experience of parental and caregiver stress.
Multidisciplinary treatment of advanced or recurrent solid pseudopapillary neoplasm of the pancreas: three case reports
Authors: Tanoue Kiyonori , Mataki Yuko , Kurahara Hiroshi , Idichi Tetsuya , Kawasaki Yota , Yamasaki Yoichi , Kita Yoshiaki , Hozaka Yuto , Oi Hideyuki , Nakajo Akihiro , Arigami Takaaki , Maemura Kosei , Ohtsuka Takao
Published: January 2022

Background

Solid pseudopapillary neoplasm (SPN) is a rare pancreatic tumor that predominantly affects young females. Prognosis is excellent; however, 10–15% of patients show metastasis at the time of surgery or develop tumor recurrence after pancreatectomy.

Case presentation

We reviewed the clinical course of three patients with advanced or recurrent SPN and subsequently underwent multidisciplinary treatment at our institution between 2002 and 2019. The primary tumor was resected in all three patients, and metastases were also resected if indicated. Intensive combined therapy, including re-resection, chemotherapy, ablation, arterial chemoembolization, and radiation therapy, allowed all patients to survive for a long time. The literature review showed that resection seems to be more effective than other treatments for metastatic SPN.

Conclusions

Multidisciplinary treatment, including resection, may improve the prognosis of patients with SPN with recurrence or metastasis.
Tumor-to-tumor metastasis: an extremely rare combination with renal cell carcinoma as the donor and a pancreatic neuroendocrine tumor as the recipient
Authors: Minezaki Shunryo , Misawa Takeyuki , Tsukayama Hiroyuki , Shibuya Makoto , Wada Keita , Sano Keiji , Mochizuki Makoto , Sasajima Yuko , Kondo Hiroshi
Published: January 2022

Background

Tumor-to-tumor metastasis is a rare phenomenon in which primary tumor cells metastasize hematogenously into another tumor. Herein, we report an extremely rare case of a renal cell carcinoma metastasis into a pancreatic neuroendocrine tumor exhibiting a tumor-to-tumor metastasis. Ours is the third reported case worldwide.

Case presentation

The patient, a 72-year-old male, was referred to our hospital for further examination and treatment due to high levels of prostate-specific antigen. A left renal tumor and pancreatic head tumor were revealed incidentally on screening computed tomography. There were suspected to be a renal cell carcinoma and primary pancreatic neuroendocrine tumor or pancreatic metastasis from the renal cell carcinoma according to preoperative examination. The left nephrectomy and subtotal stomach-preserving pancreaticoduodenectomy were performed because of the pancreatic tumor indicated for operation in either case of diagnosis. Postoperative pathological examination showed a diagnosis of clear cell renal cell carcinoma for the left renal tumor. The pancreatic tumor was diagnosed with clear cell renal cell carcinoma metastasis into the pancreatic neuroendocrine tumor, that is to say tumor-to-tumor metastasis.

Conclusion

In some cases, conservative approach is selected for pancreatic neuroendocrine tumor patients who meet some requirements. However, if such patients exhibit tumor-to-tumor metastasis which combines with renal cell carcinoma and pancreatic neuroendocrine tumor as this case, conservative approach leads to progression of renal cell carcinoma. Therefore, conceiving the possibility of tumor-to-tumor metastasis, it is necessary to carefully choose a treatment plan for pancreatic neuroendocrine tumor patients associated with renal cell carcinoma, not easily choosing conservative approach.
Fingerprint sign in Vogt-Koyanagi-Harada disease: a case series
Authors: Missaka Ruy Felippe Brito Gonçalves , Goldbaum Mauro , Machado Cleide Guimarães , Cunningham Emmett T. , Souto Fernanda Maria Silveira , Lavezzo Marcelo Mendes , da Nóbrega Priscilla Figueiredo Campos , Sakata Viviane Mayumi , Oyamada Maria Kiyoko , Hirata Carlos Eduardo , Yamamoto Joyce Hisae
Published: January 2022

Background

The tomographic finding, which has been called the "fingerprint sign" in en face reconstructions, seems to be the result of a variety of processes that cause distension of the outer plexiform layer (OPL) and the Henle fiber layer (HFL). The aim of this paper is to describe the appearance of concentric rings at the OPL/HFL interface visualized using en face reconstructions of cross-sectional optical coherence tomography images of patients with Vogt-Koyanagi-Harada disease.

Methods

Retrospective analysis of images of six eyes of three patients obtained by cross-sectional OCT imaging and en face reconstruction at the level of the OPL/HFL interface.

Results

All eyes presented with a dentate or saw-tooth pattern of the OPL/HFL interface on cross-sectional OCT with corresponding concentric rings on en face OCT reconstruction, consistent with the recently published “fingerprint sign”. Initial OPL/HFL interface changes were observed between the first and fourth months after treatment and resolution of VKHD associated serous retinal detachments. These OPL/HFL interface changes have persisted for many years following the resolution of the active inflammation.

Conclusions

Changes in the OPL/HFL interface can be identified following successful treatment of VKHD. These included both a dentate or saw-tooth pattern on cross-sectional imaging and concentric rings or the “fingerprint sign” on en face reconstructions. These changes persisted for many years despite disease quiescence.
Sidelined during COVID-19: a narrative inquiry into how simulationists experienced the pandemic
Authors: Leighton Kim , Kardong-Edgren Suzan , Jones Anna , Reedy Gabriel
Published: January 2022

Background

In the simulation community, colleagues who are no longer clinically practicing were often proximal to the COVID-19 response, not working in the frontlines of patient care. At the same time, their work as simulationists changed dramatically or was halted. This research explored the experiences of those simulationists who have clinical backgrounds but did not provide direct patient care during the initial pandemic response. The aim of this study was to allow those simulationists to share and have their stories heard.

Methods

This qualitative research used a narrative approach to answer the research question: What were the experiences of those in the simulation community who did not contribute to the frontline patient care response during the early stages of the pandemic? A semi-structured questionnaire aimed at eliciting a story was disseminated through online simulation discussion boards. Data was collected through PHONIC with options to type or speak responses. Responses were analyzed using an inductive analytical process to identify themes or patterns in the narratives.

Results

Thirty-six respondents completed the survey between August 1, 2020 and November 30, 2020. Narrative arcs were identified that illustrated the events, actions, thoughts and feelings representative of experiences shared by many simulationists. Two major themes emerged: Challenges and Opportunities. Challenges included feelings of guilt; frustration; overwhelmed, stressed and exhausted; being away from the action, being unused and underappreciated. Opportunities included leadership (evolution and innovation), personal development, and being a part of something.

Conclusions

The findings reflect a snapshot in time of how simulation was viewed and used in the world during a pandemic through the personal stories of simulationists with clinical backgrounds who did not provide direct patient care. Sharing these narratives may inform future simulation development; however, it is vitally important that the emotions are recognized and acknowledged. Managers should ensure mental health resources and support are available to all staff, including those not deployed to the frontline.
Assisted peritoneal dialysis: strategies and outcomes
Authors: Giuliani Anna , Sgarabotto Luca , Manani Sabrina Milan , Tantillo Ilaria , Ronco Claudio , Zanella Monica
Published: January 2022

Assisted peritoneal dialysis (asPD) is a modality intended for not self-sufficient patients, mainly elderly, who are not able to perform peritoneal dialysis (PD) alone and require some help to manage the treatment. In the last decades, many countries developed strategies of asPD to face with aging of dialysis population and give an answer to the increasing demand of health service for elderly. Model of asPD varies according to the type of assistants employed and intensity of assistance provided. Both health care and non-health care assistants have been used with good clinical results. A mixed model of help, using different professional figures for short time or for longer according to patients’ need, has been proved successful and cost-effective. Outcomes of asPD are reported in different ways, and the comparative effect of asPD is unclear. Quality of life has rarely been evaluated; however, patients seem to be satisfied with the assistance provided, since it allows them to both retain independence and to be relieved from the burden of self-care. Assisted PD should not be intended as a PD-favoring strategy, but as a model that allows home dialysis also in patients who would not be eligible for PD because of social, cognitive or physical barriers.
Normalized lifespan inequality: disentangling the longevity–lifespan variability nexus
Authors: Permanyer Iñaki , Shi Jiaxin
Published: January 2022

Previous studies have documented a historically strong and negative association between countries’ life expectancy (i.e., average longevity) and length-of-life inequality (i.e., variability in ages at death). The relationship between both variables might be partially explained by life expectancy increasing at a faster pace than maximal length of life, a phenomenon that mechanically compresses the age-at-death distribution and has not been taken into consideration in previous studies. In this paper, we propose a new approach to lifespan inequality measurement that accounts for the (uncertainly) bounded nature of length-of-life. Applying the new approach to the countries of the Human Mortality Database, we observe that the decline in overall lifespan variability typically associated with increases in longevity seems to stop and even reverse at higher levels of life expectancy. This suggests the emergence of worrying ethical dilemmas, whereby higher achievements in longevity would only be possible at the expense of higher lifespan variability.
Speed versus accuracy instructions in the response time concealed information test
Authors: Lubczyk Till , Lukács Gáspár , Ansorge Ulrich
Published: January 2022

The response time concealed information test (RT-CIT) can reveal that a person recognizes a relevant item (probe) among other, irrelevant items, based on slower responding to the probe compared to the irrelevant items. Thereby, if this person is concealing knowledge about the relevance of this item (e.g., recognizing it as a murder weapon), this deception can be unveiled. In the present paper, we examined the impact of a speed versus accuracy instruction: Examinees (N = 235) were either presented with instructions emphasizing a focus on speed, with instructions emphasizing a focus on accuracy, or with no particular speed or accuracy instructions at all. We found that although participants responded to the probe and the irrelevants marginally faster when they had received instructions emphasizing speed, there was no significant difference between RTs of the different experimental groups and crucially no significant difference between the probe–irrelevant RT differences either. This means that such instructions are unlikely to benefit the RT-CIT, but it also suggests that related deliberate manipulation (focusing on speed on or accuracy) is unlikely to decrease the efficiency of the RT-CIT—contributing further evidence to the RT-CIT’s resistance to faking.
COVID-19, neurovascular thrombotic problem and short summary on blood coagulation disorder: a brief review
Authors: Mungmunpuntipantip Rujittika , Wiwanitkit Viroj
Published: January 2022

COVID-19 is the present global public health problem. This respiratory viral infection can manifest atypical presentation including neurological presentations. An important neurological problem in COVID-19 is neurovascular thrombosis. The basic pathogenesis of thrombosis in neurological system is explainable by the basic principle of thrombohemostasis. A hypercoagulability is a possible problem seen in some COVID-19 cases. In this brief review, the authors summarize venous and arterial thrombosis of neurovascular system as a complication of COVID-19. The updated pathophysiology of COVID-associated blood coagulation disorder is discussed. In addition, consideration regarding new COVID-19 vaccine related thrombotic adverse event is also raised.
The impregnation of ZnO onto ZSM-5 derived from red mud for photocatalytic degradation of methylene blue
Authors: Tehubijuluw Hellna , Subagyo Riki , Kusumawati Yuly , Prasetyoko Didik
Published: January 2022

Photocatalytic degradation of Methylene Blue (MB) by zinc oxide/zeolite socony mobile-5 (ZnO/ZSM-5) composites was investigated. The ZSM-5 material was synthesized from red mud by a two-step hydrothermal method to which ZnO loadings at different mass ratios were subsequently performed. Characterizations using X-ray diffraction (XRD), Fourier transform infrared spectroscopy, and scanning electron microscopy were carried out to identify the formation of ZSM-5 and ZnO/ZSM-5. ZSM-5 and ZnO/ZSM-5 have cubic microcrystallite morphologies. ZnO loading in the ZnO/ZSM-5 composites was successfully performed and confirmed by the appearance of wurtzite peaks in the XRD spectra that matched the Joint Committee on Powder Diffraction Standards data. The presence of ZnO in ZSM-5 leading resulted in a decrease in the surface area and pore size as confirming by nitrogen adsorption-desorption isotherm experiments. The band gap of the samples was measured using UV-Vis diffuse reflectance spectroscopy. The optimum photocatalytic degradation of MB was observed at a ZnO loading of 34% w/w dubbed 34-ZnO/ZSM-5. The influence of the initial concentration of MB was also investigated at 80, 90, and 100 mg L− 1using 34-ZnO/ZSM-5 and ZSM-5. Liquid chromatography–mass spectrometry characterization was performed to analyze the degradation products.
Lateral chest wall perforator flaps in partial breast reconstruction
Authors: Orabi Ahmed , Youssef Mina M G , Manie Tamer M. , Shaalan Mohamed , Hashem Tarek
Published: January 2022

Background

Breast conserving surgery (BCS) has been a standard procedure for the treatment of breast cancer instead of mastectomy whenever possible. Lateral chest wall perforator flaps are one of the volume replacement techniques that participate in increasing the rate of BCS especially in small- to moderate-sized breasts with good cosmetic outcome. In this study, we tried to evaluate the outcome of those flaps as an oncoplastic procedure instead of the conventional flaps.

Methods

This study included 26 patients who underwent partial mastectomy with immediate reconstruction using lateral chest wall perforator flaps in the period from October 2019 to November 2020. The operative time, techniques, and complications were recorded. The cosmetic outcome was assessed 3 months post-radiation therapy through a questionnaire and photographic assessment.

Results

Lateral intercostal artery perforator (LICAP), lateral thoracic artery perforator (LTAP) and combined flaps were performed in 24, 1, and 1 patients, respectively. The mean operative time was 129.6 ± 13.2 min. The flap length ranged from 10 to 20 cm and its width from 5 to 9 cm. Overall patients’ satisfaction was observed to be 88.5% as either excellent or good and the photographic assessment was 96.2% as either excellent or good.

Conclusions

Lateral chest wall perforator flaps are reliable and safe option for partial breast reconstruction with an acceptable aesthetic outcome. In the era of oncoplastic breast surgery, they deserve to gain attention especially with the advantages of some modifications added to the classic technique.