bluuebunny/crossref_metadata_2025 huggingface dataset:
In vivo loss of tumorigenicity in a patient-derived orthotopic xenograft mouse model of ependymoma
Authors: Whitehouse Jacqueline P. , Hii Hilary , Mayoh Chelsea , Wong Marie , Ajuyah Pamela , Barahona Paulette , Cui Louise , Dholaria Hetal , White Christine L. , Buntine Molly K. , Byrne Jacob , Rodrigues da Silva Keteryne , Howlett Meegan , Girard Emily J. , Tsoli Maria , Ziegler David S. , Dyke Jason M. , Lee Sharon , Ekert Paul G. , Cowley Mark J. , Gottardo Nicholas G. , Endersby Raelene
Published: March 2023

Introduction

Ependymomas (EPN) are the third most common malignant brain cancer in children. Treatment strategies for pediatric EPN have remained unchanged over recent decades, with 10-year survival rates stagnating at just 67% for children aged 0-14 years. Moreover, a proportion of patients who survive treatment often suffer long-term neurological side effects as a result of therapy. It is evident that there is a need for safer, more effective treatments for pediatric EPN patients. There are ten distinct subgroups of EPN, each with their own molecular and prognostic features. To identify and facilitate the testing of new treatments for EPN, in vivo laboratory models representative of the diverse molecular subtypes are required. Here, we describe the establishment of a patient-derived orthotopic xenograft (PDOX) model of posterior fossa A (PFA) EPN, derived from a metastatic cranial lesion.

Methods

Patient and PDOX tumors were analyzed using immunohistochemistry, DNA methylation profiling, whole genome sequencing (WGS) and RNA sequencing.

Results

Both patient and PDOX tumors classified as PFA EPN by methylation profiling, and shared similar histological features consistent with this molecular subgroup. RNA sequencing revealed that gene expression patterns were maintained across the primary and metastatic tumors, as well as the PDOX. Copy number profiling revealed gains of chromosomes 7, 8 and 19, and loss of chromosomes 2q and 6q in the PDOX and matched patient tumor. No clinically significant single nucleotide variants were identified, consistent with the low mutation rates observed in PFA EPN. Overexpression of EZHIP RNA and protein, a common feature of PFA EPN, was also observed. Despite the aggressive nature of the tumor in the patient, this PDOX was unable to be maintained past two passages in vivo.

Discussion

Others who have successfully developed PDOX models report some of the lowest success rates for EPN compared to other pediatric brain cancer types attempted, with loss of tumorigenicity not uncommon, highlighting the challenges of propagating these tumors in the laboratory. Here, we discuss our collective experiences with PFA EPN PDOX model generation and propose potential approaches to improve future success in establishing preclinical EPN models.
Congenital second branchial cleft anomalies in children: A report of 52 surgical cases, with emphasis on characteristic CT findings
Authors: Chen Wei , Zhou Yilong , Xu Mengrou , Xu Rong , Wang Qingyu , Xu Hongming , Chen Jiarui , Li Xiaoyan
Published: March 2023

Objective

The objectives of this study was to review the clinical features and surgical treatment outcomes of congenital second branchial cleft anomalies (CSBCAs) and to investigate the characteristic computed tomography (CT) findings of CSBCAs.

Methods

We conducted a retrospective study of 52 children who were referred to Shanghai Children's Hospital from October 2014 to December 2021 diagnosed as CSBCAs.

Results

There were 36 males and 16 females. Of them, 35 patients were presented as having a skin pit at birth or discharge from the skin opening on the lateral neck, and 17 patients presented with an asymptomatic or painful mass. The typical CT features of CSBCAs included isolated and homogeneously hypodense cystic lesions surrounded by a uniformly thin, smooth wall. CSBCAs were generally located at the anteromedial border of the sternocleidomastoid muscle, posterior to the submandibular gland, and lateral to the carotid sheath. All patients were treated surgically and only one case underwent ipsilateral tonsillectomy. After a median follow-up of 30 (range 4–90) months, no recurrence or complications were observed.

Conclusions

The CSBCAs show some characteristic CT findings, which can help clinicians diagnose and plan surgical strategies. High ligation of the lesions is sufficient for complete excision of CSBCAs.
Eldecalcitol prevented OVX-induced osteoporosis through inhibiting BMSCs senescence by regulating the SIRT1-Nrf2 signal
Authors: Kou Yuying , Rong Xing , Tang Rong , Zhang Yuan , Yang Panpan , Liu Hongrui , Ma Wanli , Li Minqi
Published: March 2023

Background: Aging and oxidative stress are considered to be the proximal culprits of postmenopausal osteoporosis. Eldecalcitol (ED-71), a new active vitamin D derivative, has shown a good therapeutic effect on different types of osteoporosis, but the mechanism is unclear. This study focused on exploring whether ED-71 could prevent bone loss in postmenopausal osteoporosis by regulating the cell senescence of bone mesenchymal stem cells (BMSCs), and explaining its specific mechanism of action.

Materials and methods: An ovariectomized (OVX) rat model was established and 30 ng/kg ED-71 was administered orally once a day. The weight of rats was recorded regularly. Micro-computed tomography (CT) and histochemical staining were used to evaluate bone mass, histological parameters, and aging-related factors. Rat bone mesenchymal stem cells were extracted and cultivated in vitro. Aging cells were marked with senescence-associated β-gal (SA-β-gal) dyeing. The mRNA and protein levels of aging-related factors and SIRT1-Nrf2 signal were detected by RT-PCR, Western blot, and immunofluorescence staining. The reactive oxygen species (ROS) levels were detected by DCFH-DA staining.

Results: Compared with the Sham group, the bone volume of the ovariectomized group rats decreased while their weight increased significantly. ED-71 prevented bone loss and inhibited weight gain in ovariectomized rats. More importantly, although the expression of aging-related factors in the bone tissue increased in the ovariectomized group, the addition of ED-71 reversed changes in these factors. After extracting and in vitro culturing bone mesenchymal stem cells, the proportion of aging bone mesenchymal stem cells was higher in the ovariectomized group than in the Sham group, accompanied by a significant decrease in the osteogenic capacity. ED-71 significantly improved the bone mesenchymal stem cells senescence caused by ovariectomized. In addition, ED-71 increased the expression of SIRT1 and Nrf2 in ovariectomized rat bone mesenchymal stem cells. Inhibition of SIRT1 or Nrf2 decreased the inhibitory effect of ED-71 on bone mesenchymal stem cells senescence. ED-71 also showed a suppression effect on the reactive oxygen species level in bone mesenchymal stem cells.

Conclusion: Our results demonstrated that ED-71 could inhibit the cell senescence of bone mesenchymal stem cells in ovariectomized rats by regulating the SIRT1-Nrf2 signal, thereby preventing bone loss caused by osteoporosis.
Effect of Chinese herbal medicine therapy on risks of all-cause mortality, infections, parasites, and circulatory-related mortality in HIV/AIDS patients with neurological diseases
Authors: Chiou Jian-Shiun , Chou Chen-Hsing , Ho Mao-Wang , Tien Ni , Liang Wen-Miin , Chiu Mu-Lin , Tsai Fuu-Jen , Wu Yang-Chang , Chou I-Ching , Lu Hsing-Fang , Lin Ting-Hsu , Liao Chiu-Chu , Huang Shao-Mei , Li Te-Mao , Lin Ying-Ju
Published: March 2023

Introduction: Long-term living with human immunodeficiency virus (HIV) and/or antiretroviral therapy (ART) is associated with various adverse effects, including neurocognitive impairment. Heterogeneous neurocognitive impairment remains an important issue, affecting between 15–65% of human immunodeficiency virus infection and acquired immunodeficiency syndrome (HIV/AIDS) patients and resulting in work performance, safety, and health-related outcomes that have a heavy economic burden.

Methods: We identified 1,209 HIV/AIDS patients with neurological diseases during 2010–2017. The Kaplan–Meier method, log-rank test, and Cox proportional hazards model were used to analyze 308 CHM users and 901 non-CHM users within this population. Major CHM clusters were determined using association rule mining and network analysis.

Results and Discussion: Results showed that CHM users had a 70% lower risk of all-cause mortality (adjusted hazard ratio (aHR) = 0.30, 95% confidence interval (CI):0.16–0.58, p < 0.001) (p = 0.0007, log-rank test). Furthermore, CHM users had an 86% lower risk of infections, parasites, and circulatory-related mortality (aHR = 0.14, 95% confidence interval (CI):0.04–0.46, p = 0.001) (p = 0.0010, log-rank test). Association rule mining and network analysis showed that two CHM clusters were important for patients with neurological diseases. In the first CHM cluster, Huang Qin (HQ; root of Scutellaria baicalensis Georgi), Gan Cao (GC; root of Glycyrrhiza uralensis Fisch.), Huang Lian (HL; root of Coptis chinensis Franch.), Jie Geng (JG; root of Platycodon grandiflorus (Jacq.) A.DC.), and Huang Bai (HB; bark of Phellodendron amurense Rupr.) were identified as important CHMs. Among them, the strongest connection strength was identified between the HL and HQ. In the second CHM cluster, Suan-Zao-Ren-Tang (SZRT) and Ye Jiao Teng (YJT; stem of Polygonum multiflorum Thunb.) were identified as important CHMs with the strongest connection strength. CHMs may thus be effective in treating HIV/AIDS patients with neurological diseases, and future clinical trials are essential for the prevention of neurological dysfunction in the population.
Exposure to anticholinergic and sedative medication is associated with impaired functioning in older people with vertigo, dizziness and balance disorders—Results from the longitudinal multicenter study MobilE-TRA
Authors: Katzenberger Benedict , Koller Daniela , Strobl Ralf , Kisch Rebecca , Sanftenberg Linda , Voigt Karen , Grill Eva
Published: March 2023

Introduction: Anticholinergic and sedative medication is prescribed for various conditions in older patients. While the general association between anticholinergic and sedative medication and impaired functioning is well established, its specific role in older individuals with vertigo, dizziness, and balance disorders (VDB) is still incompletely understood. The objective of this study was to investigate, whether an exposure to anticholinergic and sedative medication is associated with lower generic and lower vertigo-specific functioning in older patients with VDB.

Methods: Data originates from the longitudinal multicenter study MobilE-TRA with two follow-ups, conducted from 2017 to 2019 in two German federal states. Exposure to anticholinergic and sedative medication was quantified using the drug burden index (DBI). Generic functioning was assessed by the Health Assessment Questionnaire Disability Index, appraising the amount of difficulties in performing activities of daily living (ADL). Vertigo-specific functioning was measured using the Vestibular Activities and Participation (VAP) questionnaire, assessing patient-reported functioning regarding activities of daily living that are difficult to perform because of their propensity to provoke VDB (Scale 1) as well as immediate consequences of VDB on activities and participation related to mobility (Scale 2). Longitudinal linear mixed models were applied to assess the association of exposure to anticholinergic and sedative medication at baseline and the level of generic and vertigo-specific functioning status over time.

Results: An overall of 19 (7 from Bavaria) primary care physicians (mean age = 54 years, 29% female) recruited 158 (59% from Bavaria) patients with VDB (median age = 78 years, 70% female). Anticholinergic and sedative medication at baseline was present in 56 (35%) patients. An exposure to anticholinergic and sedative medication at baseline was significantly associated with lower generic functioning [Beta = 0.40, 95%-CI (0.18; 0.61)] and lower vertigo-specific functioning [VAP Scale 1: Beta = 2.47, 95%-CI (0.92; 4.02)], and VAP Scale 2: Beta = 3.74, 95%-CI [2.23; 5.24]).

Conclusion: Our results highlight the importance of a close monitoring of anticholinergic and sedative medication use in older patients with VDB. When feasible, anticholinergic and sedative medication should be replaced by equivalent alternative therapies in order to potentially reduce the burden of VDB.
Relationship between body composition and upper limb physical fitness among Chinese students: 4-Year longitudinal follow-up and experimental study
Authors: Wang Qingmei , Qian Junwei , Pan Haoran , Ju Qianqian
Published: March 2023

Background: Recently, students’ fitness has been declining, and high physical fitness level is crucial in establishing optimal physical/mental health and academic performance. The purpose of this study was to explore the relationship between body composition and upper limb physical fitness and the specific aspects of low physical fitness level in Chinese students. Exploring the development and impact factors for upper limb physical fitness can provide a theoretical basis for the health management strategy of students.

Methods: Study 1 collected data from 183 male students over 4 years and used Hierarchical Linear Model (HLM) to explore the quadratic predictive role of body composition on upper limb physical fitness. To further explored which aspects of upper limb physical fitness were affected by body composition, study 2 conducted an experimental investigation among 42 male students, comparing different kinds of upper limb physical fitness within two different body composition groups.

Results: Studies found (1) from 2015 to 2018, students’ Body-Mass-Index (BMI) showed an upward trend, and BMI differences were significant from year to year. While the upper limb physical fitness showed a downward trend. There were significant differences in the number of pull-outs between 2015 and 2016, 2015 and 2017, and 2015 and 2018. (2) The quadratic term of BMI could predict the upper body physical fitness in the same year and the following year. That is, when BMI was medium, the upper body fitness of the same year and the following year was the best. (3) Chinese students with excellent body composition had greater grip strength, drape height and anaerobic power than those with average body composition.

Conclusion: In recent years, male students’ BMI has been increasing, and the upper body physical fitness has been decreasing. Furthermore, body composition can predict the upper body mass in the same year and the second year, and male students with better body composition also had greater grip strength, drape height and anaerobic power in their upper limbs.
Portable rapid detection of maize chlorotic mottle virus using RT-RAA/CRISPR-Cas12a based lateral flow assay
Authors: Lei Rong , Kuang Ruirui , Peng Xuanzi , Jiao Zhiyuan , Zhao Zhenxing , Cong Haolong , Fan Zaifeng , Zhang Yongjiang
Published: March 2023

Introduction

Maize lethal necrosis seriously threatens maize production worldwide, which was caused by coinfection by maize chlorotic mottle virus (MCMV) and a potyvirid. To effectively control maize lethal necrosis, it is vital to develop a rapid, sensitive, and specific detection method for the early diagnosis of MCMV in host plant tissues.

Methods

We established a rapid detection procedure by combining the one-step reverse-transcription recombinase-aided amplification (one-step RT-RAA) and CRISPR/Cas12a-based lateral flow assay in one tube (one-tube one-step RT-RAA/CRISPR-Cas12a), which can be implemented on a portable metal incubator at 37~42°C. Furthermore, the crude extract of total RNA from plant materials using alkaline-PEG buffer can be directly used as the template for one-step RT-RAA.

Results

The developed one-tube one-step RT-RAA/CRISPR-Cas12a lateral flow assay can detect as low as 2.5 copies of the coat protein (CP) gene of MCMV and 0.96 pg of the total RNA extracted from MCMV infected maize leaves. Furthermore, the MCMV infected maize leaves at 5 dpi having no obvious symptoms was detected as weak positive.

Discussion

The crude extraction method of total RNA from plant materials required no complicated device, and all the procedures could be implemented at room temperature and on a portable metal incubator, costing a total time of about 1h. The one-step RT-RAA reagents and CRISPR/Cas12a reagents can be lyophilized for easy storage and transportation of reagents, which makes this method more feasible for the filed detection. This method presents rapidness, robustness and on-site features in detecting viral RNA, and is a promising tool for the field application in minimally equipped laboratories.
Variation in nitrogen partitioning and reproductive stage nitrogen remobilization determines nitrogen grain production efficiency (NUEg) in diverse rice genotypes under varying nitrogen supply
Authors: Padhan Birendra K. , Sathee Lekshmy , Kumar Santosh , Chinnusamy Viswanathan , Kumar Arvind
Published: March 2023

Nitrogen (N) is an important macronutrient needed for grain yield, grain N and grain protein content in rice. Grain yield and quality are significantly determined by N availability. In this study, to understand the mechanisms associated with reproductive stage N remobilization and N partitioning to grain 2 years of field experiments were conducted with 30 diverse rice genotypes during 2019-Kharif and 2020-Kharif seasons. The experiments were conducted with two different N treatments; N deficient (N0-no external N application, available soil N; 2019-234.15 kgha-1, 2020-225.79 kgha-1) and N sufficient (N120-120 kgha-1 external N application, available soil N; 2019-363.77 kgha-1, 2020-367.95 kgha-1). N application increased the NDVI value, biomass accumulation, grain yield, harvest index and grain N accumulation. Post-anthesis N uptake and N remobilization from vegetative tissues to grain are critical for grain yield and N harvest index. Rice genotypes, Kalinga-1, BAM-4234, IR-8384-B-B102-3, Sahbhagi Dhan, BVD-109 and Nerica-L-42 showed a higher rate of N remobilization under N sufficient conditions. But, under N deficiency, rice genotypes-83929-B-B-291-3-1-1, BVD-109, IR-8384-B-B102-3 and BAM-4234 performed well showing higher N remobilization efficiency. The total amount of N remobilization was recorded to be high in the N120 treatment. The harvest index was higher in N120 during both the cropping seasons. RANBIR BASMATI, BAM-832, APO, BAM-247, IR-64, Vandana, and Nerica-L-44 were more efficient in N grain production efficiency under N deficient conditions. From this study, it is evident that higher grain N accumulation is not always associated with higher yield. IR-83929-B-B-291-3-1-1, Kalinga-1, APO, Pusa Basmati-1, and Nerica-L-44 performed well for different N use efficiency component traits under both N deficient (N0) and N sufficient (N120) conditions. Identifying genotypes/donors for N use efficiency-component traits is crucial in improving the fertilizer N recovery rate and site specific N management.
Remote sensing for field pea yield estimation: A study of multi-scale data fusion approaches in phenomics
Authors: Marzougui Afef , McGee Rebecca J. , Van Vleet Stephen , Sankaran Sindhuja
Published: March 2023

Introduction

Remote sensing using unmanned aerial systems (UAS) are prevalent for phenomics and precision agricultural applications. The high-resolution data for these applications can provide useful spectral characteristics of crops associated with performance traits such as seed yield. With the recent availability of high-resolution satellite imagery, there has been growing interest in using this technology for plot-scale remote sensing applications, particularly those related to breeding programs. This study compared the features extracted from high-resolution satellite and UAS multispectral imagery (visible and near-infrared) to predict the seed yield from two diverse plot-scale field pea yield trials (advanced breeding and variety testing) using the random forest model.

Methods

The multi-modal (spectral and textural features) and multi-scale (satellite and UAS) data fusion approaches were evaluated to improve seed yield prediction accuracy across trials and time points. These approaches included both image fusion, such as pan-sharpening of satellite imagery with UAS imagery using intensity-hue-saturation transformation and additive wavelet luminance proportional approaches, and feature fusion, which involved integrating extracted spectral features. In addition, we also compared the image fusion approach to high-definition satellite data with a resolution of 0.15 m/pixel. The effectiveness of each approach was evaluated with data at both individual and combined time points.

Results and discussion

The major findings can be summarized as follows: (1) the inclusion of the texture features did not improve the model performance, (2) the performance of the model using spectral features from satellite imagery at its original resolution can provide similar results as UAS imagery, with variation depending on the field pea yield trial under study and the growth stage, (3) the model performance improved after applying multi-scale, multiple time point feature fusion, (4) the features extracted from the pan-sharpened satellite imagery using intensity-hue-saturation transformation (image fusion) showed better model performance than those with original satellite imagery or high definition imagery, and (5) the green normalized difference vegetation index and transformed triangular vegetation index were identified as key features contributing to high model performance across trials and time points. These findings demonstrate the potential of high-resolution satellite imagery and data fusion approaches for plot-scale phenomics applications.
Mass screening of rice mutant populations at low CO2 for identification of lowered photorespiration and respiration rates
Authors: Mubarak A.N.M. , Burgess A.J. , Pyke K. , Quick W.P. , Murchie E.H.
Published: March 2023

Introduction

Identifying rice (Oryza sativa) germplasm with improved efficiency of primary metabolism is of utmost importance in order to increase yields. One such approach can be attained through screening genetically diverse populations under altered environmental conditions. Growth or treatment under low carbon dioxide (CO2) concentrations can be used as a means of revealing altered leaf photorespiration, respiration and other metabolic variants.

Methods

We developed a pipeline for very high throughput treatment of gamma- and ethyl methanesulfonate- (EMS) induced mutant populations of IR64 rice seedlings at very low CO2 for 7 days. 1050 seedlings per batch at 5th leaf stage were exposed to 60 ppm CO2 for the first day and 30 ppm for the remaining three days. Following this, putative candidates were identified by measuring chlorophyll depletion using SPAD. Screening results showed a distinct difference between the mutants and the WTs.

Results and discussion

The mean chlorophyll loss in WTs ranged from 65% to 11% respectively, whereas in the mutant lines chlorophyll loss ranged from 0 to 100%, suggesting considerable phenotypic variation. Rice mutants with a reduced chlorophyll reduction (<10%) were identified as ‘Chlorophyll retention mutants’ (CRMs) under low CO2 stress. In total, 1909 mutant lines (14,000 seedlings) were screened for chlorophyll content under 30 ppm CO2, with 26 lines selected for detailed screening. These 26 putative candidates were self-seeded to produce an M5 generation, used to determine the genetic control of the altered response to low CO2. Gas exchange of light and CO2 response revealed that there were significant variations among photosynthetic properties in two selected rice mutants. The CO2 compensation points in the absence of photorespiration and leaf respiration rates were lower than the WTs and anatomical analyses showed that CRM 29 had improved mesophyll cell area. We propose that this approach is useful for generating new material for breeding rice with improved primary metabolism.
A structural equation mediation model captures the predictions amongst the parameters of the ease of language understanding model
Authors: Homman Lina , Danielsson Henrik , Rönnberg Jerker
Published: March 2023

Objective

The aim of the present study was to assess the validity of the Ease of Language Understanding (ELU) model through a statistical assessment of the relationships among its main parameters: processing speed, phonology, working memory (WM), and dB Speech Noise Ratio (SNR) for a given Speech Recognition Threshold (SRT) in a sample of hearing aid users from the n200 database.

Methods

Hearing aid users were assessed on several hearing and cognitive tests. Latent Structural Equation Models (SEMs) were applied to investigate the relationship between the main parameters of the ELU model while controlling for age and PTA. Several competing models were assessed.

Results

Analyses indicated that a mediating SEM was the best fit for the data. The results showed that (i) phonology independently predicted speech recognition threshold in both easy and adverse listening conditions and (ii) WM was not predictive of dB SNR for a given SRT in the easier listening conditions (iii) processing speed was predictive of dB SNR for a given SRT mediated via WM in the more adverse conditions.

Conclusion

The results were in line with the predictions of the ELU model: (i) phonology contributed to dB SNR for a given SRT in all listening conditions, (ii) WM is only invoked when listening conditions are adverse, (iii) better WM capacity aids the understanding of what has been said in adverse listening conditions, and finally (iv) the results highlight the importance and optimization of processing speed in conditions when listening conditions are adverse and WM is activated.
How to promote the social–emotional competence of rural left-behind children? An empirical study based on propensity score matching
Authors: Mo Xiaolan , Shi Gaojun , Zhang Yanan , Xu Xiao , Ji Chengjun
Published: March 2023

Social–emotional competence (SEC) played an important role in promoting the physical and mental development of children, but there exist huge gaps in SEC development between rural left-behind children. This study used propensity score matching (PSM) to investigate 578 rural children about the effects of being left behind as well as individual characteristics and teacher support on their development of SEC. The results showed that being left behind had significant negative effects on the SEC of rural children. The development of SEC varies among left-behind children of different genders and length of left-behind duration. Teacher support had a significant moderating effect on the influence path of SEC, which could effectively mitigate the negative effects of left-behind children. Therefore, this study played an implicative role in studying the development of left-behind children’s SEC. The government and society should provide adequate cultural capital by completing the support system for compensating the lack of cultural capital. Schools and teachers should pay more attention to the development of left-behind children’s SEC through curriculum development and performance evaluation to create a positive atmosphere. Parents should promote SEC development for left-behind children by improving their communication and family parenting styles.
Kicking in or kicking out? The role of the individual motor expertise in predicting the outcome of rugby actions
Authors: Paolini Simone , Bazzini Maria Chiara , Rossini Marco , De Marco Doriana , Nuara Arturo , Presti Paolo , Scalona Emilia , Avanzini Pietro , Fabbri-Destro Maddalena
Published: March 2023

In sports, understanding others’ actions represents a fundamental skill that allows players to predict the outcome of teammates’ and opponents’ actions and counteract them properly. While it is well known that motor expertise sets better premises for predicting the result of an observed sports action, it remains untested whether this principle applies to a team where players cover different positions that imply different motor repertoires. To test this hypothesis, we selected rugby as a paradigmatic example in which only one or two players out of 22 train and perform placed kicks. We administered a placed kick outcome prediction task to three groups of participants, namely, rugby kickers, rugby non-kickers, and controls, thus spanning over different combinations of motor expertise and visual experience. Kickers outperformed both their non-kicking teammates and controls in overall prediction accuracy. We documented how the viewpoint of observation, the expertise of the observed kicker, and the position of the kick on the court influenced the prediction performance across the three groups. Finally, we revealed that within rugby players, the degree of motor expertise (but not the visual experience) causally affects accuracy, and such a result stands even after accounting for the level of visual experience. These findings extend the role of motor expertise in decoding and predicting others’ behaviors to sports teammates, among which every member is equipped with a position-specific motor repertoire, advocating for new motor training procedures combining the gestures to-be-performed with those to-be-faced.
Linear growth in young people with restrictive eating disorders: “Inching” toward consensus
Authors: Downey Amanda E. , Richards Alexis , Tanner Anna B.
Published: March 2023

Background

While the assessment of acute medical stability in patients with eating disorders should never be minimized, careful attention toward other specific age-related consequences of malnutrition can improve psychological outcomes and reduce long-term, potentially irreversible medical complications, like linear growth impairment.

Review

While the impact of malnutrition on linear growth is widely recognized, emerging data highlight consensus in several key areas: the time from onset to time of diagnosis, age at illness onset, pubertal stage at illness onset, and adequacy of weight restoration to achieve catch-up growth. This review provides concrete and actionable steps to help providers identify and explore deviations in expected growth and development while prioritizing early and aggressive weight restoration to provide the best opportunity for catch-up linear growth in patients with eating disorders.

Conclusion

The impact of restrictive eating disorders on growth and development cannot be overstated, particularly in pre- and peripubertal patients. While many consequences of malnutrition are reversible, the loss of genetic height potential may prove irreversible without early and aggressive weight restoration.
Out-of-pocket healthcare expenditures of Turkish households living with rare diseases
Authors: Koçkaya Güvenç , Oguzhan Gülpembe , Ökçün Selin , Kurnaz Mustafa
Published: March 2023

Introduction

This study aims to determine the out-of-pocket health expenditures of households in Turkey where individuals with rare diseases are residing.

Methods

The research population consisted registered members of associations who are members of the Rare Diseases Network. In addition to the general analysis including all participants, expenditures based on characteristics of disease holders were also calculated.

Results

A total of 439 participants were included in the analysis. We determined that special nutrition was the highest expenditure group and emergency departments were the lowest expenditure group. When all the participants were evaluated, the average cost of rare diseases was found to be Ł22,743 (€2,877). A significant relationship was found between income status and out-of-pocket health expenditures (p = 0.012).

Discussion

Policy makers should consider inclusion of special nutritional products and medical/non-medical devices used in treatment of rare diseases within the scope of reimbursement and the development of orphan drug legislation as the first actions to be taken.
A novel approach to utilizing the essential public health functions in Ireland's health system recovery and reform
Authors: McNicholas Triona , Hendrick Louise , McDarby Geraldine , Mustafa Saqif , Zhang Yu , Saikat Sohel , Jakab Zsuzsanna , Holohan Tony
Published: March 2023

This article is part of the Research Topic ‘Health Systems Recovery in the Context of COVID-19 and Protracted Conflict.’

The COVID-19 pandemic presented a challenge to health systems and exposed weaknesses in public health capacities globally. As Ireland looks to recovery, strengthening public health capacities to support health systems resilience has been identified as a priority. The Essential Public Health Functions (EPHFs) provide an integrated approach to health systems strengthening with allied sectors and their operationalization supports health systems and multi-sectoral engagement to meet population needs and anticipate evolving demands. The Health Systems Resilience team (World Health Organization, HQ) in collaboration with the Department of Health (Ireland) developed a novel approach to the assessment of the EPHFs in Ireland. The approach involved a strategic and focused review of the delivery and consideration of EPHFs in relation to policy and planning, infrastructure, service delivery, coordination and integration, monitoring and evaluation and learning. Informed by a literature review and key document search, key stakeholder mapping and key informant interviews, lessons learned from experience with COVID-19 nationally and internationally, strengths as well as potential areas of improvement to optimize delivery of EPHFs were identified. Mapping of the EPHFs in Ireland revealed that there is evidence of delivery of all 12 EPHFs to varying degrees; however a number of challenges were identified, as well as numerous strengths and opportunities. Recommendations to optimize the delivery of EPHFs in Ireland include to integrate and coordinate EPHFs, increase the visibility of the public health agenda, leverage existing mechanisms, recognize and develop the workforce, and address issues with the Health Information System. There is a public health reform process currently underway in Ireland, with some of these recommendations already being addressed. The findings of this process can help further inform and support the reform process. Given the current focus on strengthening public health capacities globally, the findings in Ireland have applicability and relevance in other WHO regions and member states for health systems recovery and building back better, fairer and more resilient health systems.
Internet use and depression among Chinese older adults: The mediating effect of interpersonal relationship
Authors: Nan Yan , Xie Yating , Hu Yuqun
Published: March 2023

The number of elderly Internet users has increased significantly in the past few years, and the Internet has greatly changed the way that older adults access information and communicate. Generally, those who regularly use the Internet may expand their range of interpersonal interactions, which has positive effects on their health. Depression is the leading cause of ill health, and is closely related to people's mental health. We sought to investigate whether internet use help reduce depression level among older adults. Using data from the 2020 China Family Panel Studies (CFPS), a total of 4,714 respondents were included to explore the effects of Internet use on the elderly's depression levels in China, along with the mediating role of interpersonal relationship in the above relationship. Regression results indicated that Internet use significantly reduced depression levels among the elderly. Further analysis showed that different Internet usage had different effects on depression among the elderly. Wechat chatting, video browsing, and online shopping were positively correlated with lower levels of depression. However, playing online games and online learning did not predict reduced levels of depression. Moreover, interpersonal relationship mediated the relationship between Internet use and depression levels. Internet use was associated with a higher level of interpersonal relationship, which in turn reduced depression levels in older adults. Regarding gender and regional differences, the coefficient of Internet use for urban older adults was significantly negative at 0.001 level, while it was not significant for rural older adults. A mediating effect of interpersonal relationship between Internet use and depression levels was only found for male elderly. To reduce the level of depression and promote mental health in the course of aging, Internet use and the improvement of interpersonal relationships merit special attention.
Game-based learning in medical education
Authors: Xu Maosen , Luo Yong , Zhang Yu , Xia Ruolan , Qian Hong , Zou Xiuhe
Published: March 2023

At present, medical education is rapidly evolving. Game-based learning (GBL) has been gradually used for education, and several innovations have emerged. The emergence of serious games and gamification provides alternative approaches for educators to improve the medical teaching process. Both serious games and gamification exert their education-promoting function by providing the possibility of combining learning activities such as feedback, testing, and spaced repetition with active participation and autonomy as well as positive experiences for students. Developing effective GBL modalities has the potential to bring immersive experiences for medical students and improve their study outcomes. Herein, we reviewed recent studies employing GBL in medical education, including serious games and gamification teaching. Furthermore, we also discussed the effectiveness and limitations of GBL to suggest future directions for the development and application of GBL in medical education.
Reflections on medical student evaluations of a public health clerkship
Authors: Rahma Azhar T. , Ádám Balázs , Abdullahi Aminu S. , Sheek-Hussein Mohamud , Shaban Sami , AlShamsi Mouza , AlKhori Salama , Nauman Javaid , Grivna Michal
Published: March 2023

Introduction

The COVID-19 pandemic demonstrated the need for skilled medical practitioners in public health, and outbreak investigations. The College of Medicine and Health Sciences at the United Arab Emirates University (UAEU) introduced a clerkship in public health constituting theoretical and practical sessions to 5th year medical students in 2015. The aim of this study is to explore the satisfaction of the students with the public health clerkship which is crucial for the assessment and reformation of the taught curriculum.

Methods

A cross-sectional, post-evaluation analysis was conducted from the period 2015–2022. The evaluation questionnaire was conducted via an online university system. The survey contained 5 themes: pre-course instructions, structure of the clerkship, academic staff, activities, and learning outcomes. Ethics approval was secured from the Social-IRB of the UAEU. We used SPSS version 26 to analyze the data using independent t-test and ANOVA.

Results

One hundred and seventy four students (27.4% response rate) participated in the study. Overall, the students had an average satisfaction score of 2.86 out of 4. The majority of the students reported having a good understanding of public health (93.7%), improving their oral presentation skills (91.2%), and developing new skills (87.2%). Furthermore, more than 9 in 10 students (96.1%) reported that the program expanded their knowledge, skills, and confidence. The mass (90.2%) of students agreed that the clerkship content was covered in sufficient depth, majority of the students agreed that they had received enough information about the clerkship before it started (74.6%), majority of the students agreed that the faculty were interested in their personal development (86.1%) The students who completed the clerkship prior to the COVID-19 pandemic had a statistically significant (P = 0.02) higher average rating (72.8%) than students who completed the clerkship during the pandemic (71.1%).

Discussion

Medical students at the UAEU were satisfied with the activities and delivery of the public health clerkship and found it rewarding. Conducting needs assessment and proposal writing provided them with the knowledge, skills, and confidence to conduct research in their career. These findings may be useful in helping and support other institutes to plan and develop a clerkship in the public health.
Important role of the government in reducing pesticide use and risk sustainably in Thailand: Current situation and recommendations
Authors: Sapbamrer Ratana , Kitro Amornphat , Panumasvivat Jinjuta , Assavanopakun Pheerasak
Published: March 2023

Agriculture is an important aspect of Thailand's GDP and development. It ranks as the 8th largest exporter in the world, however, pesticide use associated with the agriculture is ranked 18th in the world and 5th in Asia. Previous studies have clearly stated that pesticides are a threat to human health and the environment. The government is now making efforts to address pesticide use and the health impacts of pesticide use, however, these efforts are still in need of completion. This paper aims to summarize: (1) the current situation with regard to pesticide use in Thailand; (2) the current situation with regard to pesticide poisoning in Thailand; and (3) important role of government in reducing pesticide use and risk sustainably in Thailand: current situation and recommendations. This article suggests that government is a significant driver for reducing pesticide use and risk sustainably and the establishment of push and pull policies, legislation, and strategies. The government needs to strictly adhere to international conventions. Introducing a pesticide tax is essential to reduce redundant pesticide use. Updating of the current act, specific regulations with regard to pesticides and strict enforcement are urgently required. Licensing pesticide applicators should be implemented continuously. Promotion of alternative pest management is needed by supportive production inputs, technology, and markets as well as the development of a monitoring and certification system. Educating consumers on how to choose safe agricultural products and reduce risk from pesticide residues in the products is also necessary. All approaches should be implemented simultaneously and instantly. Importantly, the government needs to cooperate with agricultural sectors, health and environmental sectors, private sectors, as well as food industries to tackle complicate issues in a sustainable manner and lower pesticide use and risk sustainably in Thailand.
Miniaturized NIR spectroscopy and chemometrics: A smart combination to solve food authentication challenges
Authors: Amirvaresi Arian , Parastar Hadi
Published: March 2023

Over the years, technology has allowed more accurate, more effective, and prompt food integrity assessments to assure the quality and authenticity of food material. Thanks to the development of portable and hand-held near infrared (NIR) as a rapid, reliable, non-destructive, and user-friendly instrument, on-site food analysis is provided with more feasibility. However, miniaturized NIR devices have some significant challenges due to the presence of varying noise resources which can lead to misinterpretation. In this context, chemometric methods with the capability of resolution, identification, classification, and calibration play a pivotal role in achieving precise and in-depth comprehension of the data. In the present mini-review, we will discuss miniaturized NIR instrumentation, some chemometric concepts, and introduce the most popular algorithm in food authentication problem. The main feature of this review is avoiding mathematical details as much as possible to make the material accessible to a broad audience but highlighting the key features of chemometric methods with some simple illustrative examples in the scope of food authenticity.
High-altitude vertical wind profile estimation using multirotor vehicles
Authors: McConville Alexander , Richardson Thomas
Published: March 2023

Capturing vertical profiles of the atmosphere and measuring wind conditions can be of significant value for weather forecasting and pollution monitoring however, collecting such data can be limited by current approaches using balloon-based radiosondes and expensive ground-based sensors. Multirotor vehicles can be significantly affected by the local wind conditions, and due to their under-actuated nature, the response to the flow is visible in the changes in the orientation. From these changes in orientation, wind speed and direction estimates can be determined, allowing accurate estimation with no additional sensors. In this work, we expand on and improve this method of wind speed and direction estimation and incorporate corrections for climbing flight to improve estimation during vertical profiling. These corrections were validated against sonic anemometer data before being used to gather vertical profiles of the wind conditions around Volcan De Fuego in Guatemala up to altitudes of 3000 m Above Ground Level (AGL). From the results of this work, we show we can improve the accuracy of multirotor wind estimation in vertical profiling through our improved model and some of the practical limitations of radiosondes that can be overcome through the use of UAS in this application.
The key players of dysbiosis in Noma disease; A systematic review of etiological studies
Authors: Uzochukwu Ifeanyi , Moyes David , Proctor Gordon , Ide Mark
Published: March 2023

Noma is a rapidly progressing periodontal disease with up to 90% mortality in developing countries. Poor, immunocompromised and severely malnourished children (2 to 6 years old) are mostly affected by Noma. Prevention and effective management of Noma is hindered by the lack of sufficient cohesive studies on the microbial etiology of the disease. Research efforts have not provided a comprehensive unified story of the disease. Bridging the gap between existing studies gives an insight on the disease pathogenesis. This current systematic review of etiological studies focuses on the key players of dysbiosis in Noma disease. This review was performed in accordance with the Preferred Reporting Items for Systemic review and Meta-Analyses (PRISMA) statement. Web of Science, MEDLINE via PubMed, Cochrane Library, Scopus, and Science Direct were searched electronically for clinical trials which applied culture dependent or molecular techniques to identify oral microbiota from Noma patients. Trials which involved periodontal diseases except Noma were excluded. After screening 275 articles, 153 full-texts articles were assessed for eligibility of which eight full text articles were selected for data extraction and analysis. The results show that 308 samples from 169 Noma participants (6 months to 15 years old) have been used in clinical trials. There was some variance in the microbiome identified due to the use of 3 different types of samples (crevicular fluid, subgingival plaque, and swabbed pus) and the ambiguity of the stage or advancement of Noma in the studies. Other limitations of the studies included in this review were: the absence of age-matched controls in some studies; the constraints of colony morphology as a tool in distinguishing between virulent fusobacterium genus at the species level; the difficulty in culturing spirochaetes in the laboratory; the choice of primers in DNA amplification; and the selection of probe sets in gene sequencing. This systematic review highlights spirochaetes and P. intermedia as putative trigger organisms in Noma dysbiosis, shows that F. nucleatum promotes biofilms formation in late stages of the disease and suggests that future studies should be longitudinal, with high throughput genome sequencing techniques used with gingival plaque samples from early stages of Noma.