bluuebunny/crossref_metadata_2025 huggingface dataset:
A importância do planejamento de hortas em escolas de educação infantil
Authors: Baisch Jéssica Streck , Anschau Jaqueline Rambo , Zappe Janessa Aline , Wollmann Ana Rita Pereira , Machado Bruno Luan Rosa , Frescura Kelen Dal-Souto , Frescura Viviane Dal-Souto
Published: March 2020

Creating spaces to work on themes such as environment, sustainability and healthy eating is a practice adopted in early kindergarten schools. In this sense, the garden is a tool for working on various themes and developing students' commitment to the environment and to food production using sustainable techniques. Thus, this work aimed to plan and implement a vegetable garden at Escola Municipal de Educação Infantil Sol Criança (Santiago, Rio Grande do Sul) e no Colégio Totem (Cachoeira do Sul, Rio Grande do Sul). The planning of the garden was carried out with the help of teachers and pedagogical coordination of the schools involved, in order to provide greater interaction of students with the garden. For this, the beds were prepared with used tires, so that all the space was accessible to students. Members of the Horta Viva at School Extension Project prepare the flowerbeds and on the day of the vegetable planting the students and teachers of the schools participated in the activity. Activities were also planned to be developed with students during the school year. The garden has allowed for greater integration between students, and the space will serve for various teaching-learning activities in schools.
Incontinência urinária e sua relação com variáveis obstétricas
Authors: Silva Jaíza Marques Medeiros e , Silva Maria do Socorro Barbosa e
Published: March 2020

O estudo objetivou determinar a prevalência da incontinência urinária (IU) na gestação e identificar sua associação com variáveis obstétricas. Estudo transversal em que foram incluídas gestantes com rotina de pré-natal, na faixa etária de 18 e 35 anos, com gestação de baixo risco e no segundo ou terceiro trimestre. Foram utilizados o questionário sociodemográfico e o International Consultation on Incontinence Questionnaire - Short Form. As gestantes foram agrupadas de acordo com a presença ou não de sintomas de perda urinária. Os testes estatísticos utilizados foram o Teste de Fisher e Mann-Whitney. Prevalência de IU foi 36,7%. Na análise bivariada, não foi encontrada nenhuma associação significativa entre as variáveis obstétricas e a IU, porém na análise das variáveis contínuas, houve associação significativa entre paridade e IU. É necessária atuação dos profissionais da saúde no combate a IU no período gestacional, identificando os possíveis fatores de risco para o aparecimento dessa patologia.
Excesso de peso, obesidade e educação no Brasil
Authors: Dias Júnior Claudio Santiago , Verona Ana Paula
Published: March 2020

Este estudo tem como objetivo descrever a prevalência do excesso de peso e da obesidade no Brasil em 2006 e 2016, segundo o sexo e escolaridade e verificar se a escolaridade ainda é um fator de proteção para as mulheres, e se entre os homens, o paradoxo escolaridade/obesidade ainda permanece. Para isso, este trabalho utilizou as informações da pesquisa “Vigilância de Fatores de Risco e Proteção para Doenças Crônicas por Inquérito Telefônico” (VIGITEL) dos anos de 2006 e 2016. Ao contrário das evidências recentes encontradas nos EUA e Europa, a educação parece não ter um papel protetor para os homens brasileiros no que se refere ao excesso de peso e obesidade. Em relação às mulheres, essa variável ainda é importante, pois parece ter um papel protetor para o ganho de peso, embora já seja possível identificar que mesmo entre as mais escolarizadas, o aumento da prevalência do excesso de peso e da obesidade no período ocorreu.
Perfil do uso de medicamentos por idosos da Estratégia Saúde da Família de Porto Alegre
Authors: Andrade Camila Pereira de , Engroff Paula , Sgnaolin Vanessa , Gomes Irenio , Terra Newton Luiz
Published: March 2020

Objetivo: Avaliar o perfil farmacológico dos idosos atendidos pela Estratégia Saúde da Família (ESF). Métodos: Estudo transversal, coletado de forma prospectiva em uma amostra aleatória da população idosa cadastrada na ESF de Porto Alegre/RS. Os idosos foram entrevistados nas residências pelos agentes comunitários de saúde que aplicaram um questionário geral, contendo informações sobre medicamentos. Os dados farmacoterapêuticos foram utilizados para identificar os princípios ativos, classificados pela Anatomical Therapeutical Chemical Classification System. Resultados: Foram incluídos 761 participantes com idade média de 77,1±10,3 anos, representados na sua maioria por mulheres (63,9%), cor branca (64,7%) e primeiro grau incompleto (66,8%). A média de medicamentos utilizados foi de 4,1±3,1 e a prevalência 85,0%. Os grupos anatômicos mais utilizados foram: sistema cardiovascular (80,1%), sistema digestivo e metabolismo (56,9%) e sistema nervoso (46,8%). Em relação ao entendimento, 75,4% dos idosos reconheciam o medicamento pelas características da embalagem e 53,4% adquiriam na rede pública. Não aderiam ao tratamento farmacológico 66,8%. Conclusão: Como os idosos são os que mais utilizam medicamentos, estudos com essa abordagem podem contribuir para formulação de estratégias de atenção a essa população.
Sugestões de melhorias na prestação da atenção fisioterapêutica ofertada no contexto público municipal: revisão integrativa da literatura
Authors: Petermann Xavéle Braatz , Miranda Fernanda Alves Carvalho de , Lehnhart Eliete Dos Reis
Published: March 2020

Este estudo tem como objetivo propor sugestões de melhorias na prestação dos serviços da atenção fisioterapêutica ofertada no contexto público municipal. Caracteriza-se como uma revisão integrativa da literatura. A busca bibliográfica foi realizada em fevereiro de 2019 na base de dados LILACS, Scielo, MEDLINE e Google Scholar nos idiomas inglês, português e espanhol nos últimos dez anos (2008-2018). Os termos utilizados para a busca dos estudos foram: “satisfação dos usuários” e “fisioterapia”. Foram encontrados 502 estudos nas bases de dados, ao se aplicarem os critérios de elegibilidade, sete artigos foram selecionados para análise. Os dados obtidos foram explorados por meio da análise descritiva. A partir das barreiras identificadas nos estudos - distância geográfica do serviço de fisioterapia, a burocracia para agendamento, a disponibilidade de vagas, o número insuficiente de profissionais, o usuário desconhecer o nome do terapeuta e não receber informações sobre sua saúde - propõe-se como sugestões de melhorais na prestação dos serviços de atenção fisioterapêutica ofertada no contexto público municipal: fortalecer a atuação da fisioterapia na Atenção Primária à Saúde, promover a humanização do cuidado em saúde e instituir um protocolo de avaliação fisioterapêutica. Os achados revelam que a avaliação da satisfação dos usuários pode ser considerada como um dispositivo potencial de gestão e planejamento para a qualificação do atendimento de fisioterapia no contexto público municipal de acordo com as necessidades de saúde da população.
Efeito da fisioterapia aquática na qualidade de vida e na funcionalidade do membro superior de mulheres mastectomizadas
Authors: Schlemmer Gessica Bordin Viera , Vendrusculo Alecsandra Pinheiro , Ferreira Amanda Dias de Macedo
Published: March 2020

Objetivo: Avaliar o efeito da fisioterapia aquática na qualidade de vida e na funcionalidade do membro superior de mulheres mastectomizadas. Método: A amostra foi composta por cinco mulheres mastectomizadas, todas com mastectomia radical e uma paciente com mastectomia bilateral (radical e radical modificada). Foi avaliado a qualidade de vida e a funcionalidade através de questionários, a amplitude de movimento através da goniometria e o edema através do deslocador de água. Os dados foram analisados de forma descritiva, através de média e desvio padrão e foram elaborados gráficos para melhor visualização dos resultados. Resultados: Verificou-se melhora na amplitude de movimento, diminuindo relativamente o edema, melhorando assim a funcionalidade e a qualidade de vida. Conclusão: A fisioterapia aquática é um bom recurso para tratar estas pacientes, principalmente sobre a amplitude de movimento, a qualidade de vida e a funcionalidade.
Efeitos da fotobioestimulação e estimulação elétrica na cicatrização e qualidade de vida de indivíduos com úlceras cutâneas: terapias isoladas e associadas
Authors: Polachini Carla Roberta Nunes , Berni Viviane Bohrer , Bellé Jéssica Michelon , Oliveira Daiane Flores de , Saccol Michele Forgiarini
Published: March 2020

Objetivo: investigar os efeitos de intervenções fisioterapêuticas isoladas e associadas com laser de baixa potência e microcorrente no reparo cicatricial e qualidade de vida em indivíduos com úlceras cutâneas de diferentes etiologias. Métodos: Estudo quase-experimental realizado no Hospital Universitário de Santa Maria. A amostra constituiu-se de nove pacientes com úlceras cutâneas. Esses foram divididos em três grupos: laser (n=3), microcorrente (n=3) e laser associado a microcorrente (n=3), com aplicação de laser (660nm; modo contínuo; P= 30mW; densidade de energia= 4J/cm2) e microcorrente (f= 130Hz; i= 300μA; t= 30 minutos), totalizando 15 sessões de fisioterapia em torno de 45 dias (maio a julho). Avaliou-se a área da úlcera cutânea e processo de cicatrização (uso de régua associada a fotogrametria), dor (Escala Visual analógica), além de sensibilidade (por meio de estesiômetro). Também, foi aplicado o questionário de Qualidade de Vida WHOQOL-bref. Todas essas variáveis e analises ocorreram em três momentos (Pré, 10ª e 15ª intervenção). Resultados: Houve uma redução significativa no comprimento da úlcera cutânea, verificou-se no pré 2,80 (1,45-4,72) e na 15ª aplicações 2,10 (0-4,27) (p=0,0010), bem como a largura também reduziu na 15ª intervenção (p=0,0011). Assim, a área da ferida apresentou uma significante redução na 15ª intervenção, de 17,8 (5,70-30,76) para 7,17 (0-21,30) (p = 0,0023). Além disso, constatou-se uma diferença significativa na qualidade de vida dos pacientes já a partir da 10ª sessão (p<0,05). Conclusões: O uso da fotobioestimulação e da microcorrente aplicados de forma isolada e associada otimizaram o reparo tecidual das úlceras cutâneas de diversas etiologias promovendo uma redução na área da ferida com consequente melhora na cicatrização e na qualidade de vida dos pacientes. Nesse contexto, esta pesquisa agrega valor à pratica clínica fisioterapêutica no tratamento para feridas.
Benefits of High-Volume Medical Oncology Care for Noncurable Pancreatic Adenocarcinoma: A Population-Based Analysis
Authors: Hallet Julie , Davis Laura , Mahar Alyson , Mavros Michail , Beyfuss Kaitlyn , Liu Ying , Law Calvin H.L. , Earle Craig , Coburn Natalie
Published: March 2020

Background:Although pancreatic adenocarcinoma (PA) surgery performed by high-volume (HV) providers yields better outcomes, volume–outcome relationships are unknown for medical oncologists. This study examined variation in practice and outcomes in noncurative management of PA based on medical oncology provider volume.Methods:This population-based cohort study linked administrative healthcare datasets and included nonresected PA from 2005 through 2016. The volume of PA consultations per medical oncology provider per year was divided into quintiles, with HV providers (≥16 patients/year) constituting the fifth quintile and low-volume (LV) providers the first to fourth quintiles. Outcomes were receipt of chemotherapy and overall survival (OS). The Brown-Forsythe-Levene (BFL) test for equality of variances was performed to assess outcome variability between provider-volume quintiles. Multivariate regression models were used to examine the association between management by HV provider and outcomes.Results:A total of 7,062 patients with noncurable PA consulted with medical oncology providers. Variability was seen in receipt of chemotherapy and median survival based on provider volume (BFL,P<.001 for both), with superior 1-year OS for HV providers (30.1%; 95% CI, 27.7%–32.4%) compared with LV providers (19.7%; 95% CI, 18.5%–20.6%) (P<.001). After adjustment for age at diagnosis, sex, comorbidity burden, rural residence, income, and diagnosis period, HV provider care was independently associated with higher odds of receiving chemotherapy (odds ratio, 1.19; 95% CI, 1.05–1.34) and with superior OS (hazard ratio, 0.79; 95% CI, 0.74–0.84).Conclusions:Significant variation was seen in noncurative management and outcomes of PA based on provider volume, with management by an HV provider being independently associated with superior OS and higher odds of receiving chemotherapy. This information is important to inform disease care pathways and care organization. Cancer care systems could consider increasing the number of HV providers to reduce variation and improve outcomes.
Quantifying the Survival Benefits of Oncology Drugs With a Focus on Immunotherapy Using Restricted Mean Survival Time
Authors: Rahmadian Amanda Putri , Delos Santos Seanthel , Parshad Shruti , Everest Louis , Cheung Matthew C. , Chan Kelvin K.
Published: March 2020

Background: Restricted mean survival time (RMST) overcomes limitations of current measures of survival benefits because it directly captures information of the entire area under Kaplan-Meier survival curves. Using RMST difference (absolute survival benefit) and RMST ratio (relative survival benefit), we quantified the magnitude of survival benefits of recent oncology drugs and compared immunotherapies with nonimmunotherapies. Methods: Kaplan-Meier curves were extracted from phase II/III randomized controlled trials used by the FDA for oncology drug approvals from January 2011 through November 2017 with overall survival (OS) or progression-free survival (PFS) as primary endpoints. RMST differences, ratios, and their 95% confidence intervals were meta-analyzed to estimate absolute and relative survival benefits of contemporary oncology drugs and to compare immunotherapies with nonimmunotherapies. Meta-regression was conducted to adjust for potential confounders. Results: Ninety-four trials with a total of 51,639 patients were included. Overall absolute survival benefits (RMST differences) were 1.55 months for OS (95% CI, 1.32–1.77) and 2.99 months for PFS (95% CI, 2.65–3.33). Overall relative survival benefits (RMST ratios) were 1.11 for OS (95% CI, 1.09–1.13) and 1.42 for PFS (95% CI, 1.36–1.48). Immunotherapy absolute PFS benefit was less than that of nonimmunotherapy (1.56 vs 3.23 months), whereas immunotherapy absolute OS benefit was larger than that of nonimmunotherapy by 0.59 months (2.02 vs 1.43 months). Adjusted OS RMST difference was 0.91 months greater for immunotherapy than for nonimmunotherapy after adjusting for confounders. Conclusions: Absolute survival benefits of recent oncology drugs are modest. Survival benefits of immunotherapies are not dramatically superior to those of nonimmunotherapies. Routine reporting and use of RMST may help patients, physicians, and payers make more informed and responsible decisions regarding the care of patients with cancer.
Metastatic Versus Osteoporotic Vertebral Fractures on MRI: A Blinded, Multicenter, and Multispecialty Observer Agreement Evaluation
Authors: Arana Estanislao , Kovacs Francisco M. , Royuela Ana , Asenjo Beatriz , Nagib Fatima , Pérez-Aguilera Sandra , Dejoz María , Cabrera-Zubizarreta Alberto , García-Hidalgo Yolanda , Estremera Ana , _ _
Published: March 2020

Background: MRI is assumed to be valid for distinguishing metastatic vertebral fractures (MVFs) from osteoporotic vertebral fractures (OVFs). This study assessed (1) concordance between the image-based diagnosis of MVF versus OVF and the reference (biopsy or follow-up of >6 months), (2) interobserver and intraobserver agreement on key imaging findings and the diagnosis of MVF versus OVF, and (3) whether disclosing a patient’s history of cancer leads to variations in diagnosis, concordance, or agreement. Patients and Methods: This retrospective cohort study included clinical data and imaging from 203 patients with confirmed MVF or OVF provided to 25 clinicians (neurosurgeons, radiologists, orthopedic surgeons, and radiation oncologists). From January 2018 through October 2018, the clinicians interpreted images in conditions as close as possible to routine practice. Each specialist assessed data twice, with a minimum 6-week interval, blinded to assessments made by other clinicians and to their own previous assessments. The kappa statistic was used to assess interobserver and intraobserver agreement on key imaging findings, diagnosis (MVF vs OVF), and concordance with the reference. Subgroup analyses were based on clinicians’ specialty, years of experience, and complexity of the hospital where they worked. Results: For diagnosis of MVF versus OVF, interobserver agreement was fair, whereas intraobserver agreement was substantial. Only the latter improved to almost perfect when a patient’s history of cancer was disclosed. Interobserver agreement for key imaging findings was fair or moderate, whereas intraobserver agreement on key imaging findings was moderate or substantial. Concordance between the diagnosis of MVF versus OVF and the reference was moderate. Results were similar regardless of clinicians’ specialty, experience, and hospital category. Conclusions: When MRI is used to distinguish MVF versus OVF, interobserver agreement and concordance with the reference were moderate. These results cast doubt on the reliability of basing such a diagnosis on MRI in routine practice.
Bladder Cancer, Version 3.2020, NCCN Clinical Practice Guidelines in Oncology
Authors: Flaig Thomas W. , Spiess Philippe E. , Agarwal Neeraj , Bangs Rick , Boorjian Stephen A. , Buyyounouski Mark K. , Chang Sam , Downs Tracy M. , Efstathiou Jason A. , Friedlander Terence , Greenberg Richard E. , Guru Khurshid A. , Guzzo Thomas , Herr Harry W. , Hoffman-Censits Jean , Hoimes Christopher , Inman Brant A. , Jimbo Masahito , Kader A. Karim , Lele Subodh M. , Michalski Jeff , Montgomery Jeffrey S. , Nandagopal Lakshminarayanan , Pagliaro Lance C. , Pal Sumanta K. , Patterson Anthony , Plimack Elizabeth R. , Pohar Kamal S. , Preston Mark A. , Sexton Wade J. , Siefker-Radtke Arlene O. , Tward Jonathan , Wright Jonathan L. , Gurski Lisa A. , Johnson-Chilla Alyse
Published: March 2020

This selection from the NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines) for Bladder Cancer focuses on the clinical presentation and workup of suspected bladder cancer, treatment of non–muscle-invasive urothelial bladder cancer, and treatment of metastatic urothelial bladder cancer because important updates have recently been made to these sections. Some important updates include recommendations for optimal treatment of non–muscle-invasive bladder cancer in the event of a bacillus Calmette-Guérin (BCG) shortage and details about biomarker testing for advanced or metastatic disease. The systemic therapy recommendations for second-line or subsequent therapies have also been revised. Treatment and management of muscle-invasive, nonmetastatic disease is covered in the complete version of the NCCN Guidelines for Bladder Cancer available at NCCN.org. Additional topics covered in the complete version include treatment of nonurothelial histologies and recommendations for nonbladder urinary tract cancers such as upper tract urothelial carcinoma, urothelial carcinoma of the prostate, and primary carcinoma of the urethra.
NCCN Guidelines Insights: Management of Immunotherapy-Related Toxicities, Version 1.2020
Authors: Thompson John A. , Schneider Bryan J. , Brahmer Julie , Andrews Stephanie , Armand Philippe , Bhatia Shailender , Budde Lihua E. , Costa Luciano , Davies Marianne , Dunnington David , Ernstoff Marc S. , Frigault Matthew , Kaffenberger Benjamin H. , Lunning Matthew , McGettigan Suzanne , McPherson Jordan , Mohindra Nisha A. , Naidoo Jarushka , Olszanski Anthony J. , Oluwole Olalekan , Patel Sandip P. , Pennell Nathan , Reddy Sunil , Ryder Mabel , Santomasso Bianca , Shofer Scott , Sosman Jeffrey A. , Wang Yinghong , Weight Ryan M. , Johnson-Chilla Alyse , Zuccarino-Catania Griselda , Engh Anita
Published: March 2020

The NCCN Guidelines for Management of Immunotherapy-Related Toxicities provide interdisciplinary guidance on the management of immune-related adverse events (irAEs) resulting from cancer immunotherapy. These NCCN Guidelines Insights describe symptoms that may be caused by an irAE and should trigger further investigation, and summarize the NCCN Management of Immunotherapy-Related Toxicities Panel discussions for the 2020 update to the guidelines regarding immune checkpoint inhibitor–related diarrhea/colitis and cardiovascular irAEs.
Genotyping of Ukrainian common wheat cultivars using the marker of the Lr48 gene conferring moderate resistance to leaf rust
Authors: Karelov A. V. , Kozub N. A. , Sozinov I. A.
Published: March 2020

Aim. Common wheat (Triticum aestivum L.) is one of the most important and widely cultivated crops over the world. For a lot of wheat diseases introduction of resistance genes is considered to be the most rational way to diminish yield losses and control spread of causal agents. The aim of this research was to study a sample of Ukrainian common wheat cultivars with the use of the molecular genetic marker for the Lr48 gene. Methods. DNA samples of 46 common wheat cultivars developed in the Remeslo Myronivka Institute of Wheat (RMIW) of National Academy of Agrarian Sciences jointly with the Institute of Plant Physiology and Genetics of National Academy of Sciences of Ukraine were analyzed with the use of the marker IWB70147. Results. It was revealed that 15 out of 46 (or 32.6 %) cultivars carried resistance-associated allele of the marker. Conclusions. It was revealed that the resistance-associated allele of the marker of the Lr48 gene is present in Ukrainian common wheat cultivars developed in the Forrest Steppe zone of Ukraine. The possible source of the resistance allele is „Mironovskaya 808‟ which is in the pedigree of many Ukrainian and world wheat cultivars. The data obtained in this research can be used in breeding programs to select sources of moderate adult plant resistance. Cultivars „Yuviliar Myronivskii‟, „Volodarka‟ and „Pamyati Remesla‟ with adult leaf rust resistance conferred by the Lr48 gene also carry resistance associated allele of the Lr34/Yr18/Pm38/Sr57/Bdv1 gene.Keywords: molecular markers, wheat, resistance genes, adult plant resistance.
Analysis of ukrainian and foreign wheat samples for the presence of stem rust resistance genes using molecular markers
Authors: Ivashchuk B. V. , Pirko Ya. V. , Spivak S. I. , Yemets A. I. , Kalafat L. O. , Karelov A. V. , Kozub N. O. , Blume Ya. B.
Published: March 2020

Aim. The aim of the study was a search of Sr2 and Sr33 gene resistance alleles in foreign and domestic wheat samples using molecular genetic markers. Methods. The PCR with provided molecular genetic markers and with specific primers of own design was used; CTAB method was used for DNA extraction; electrophoresis in agarose gel was used. Results. The analysis of 16 wheat samples using molecular genetic markers for stem rust resistance genes Sr2 and Sr33 was performed. In general, among 16 analyzed samples only two have Sr2 gene resistance allele, and another two – Sr33 gene resistance allele. There were no alleles of resistance of Sr-genes among the sorts of Ukrainian selection (Kharkivska 6 and Kharkivska 12), which were used in this study. Conclusions. There were four samples among the wheat lines/sorts with valuable traits of domestic and foreign selection that have Sr2 and Sr33 gene resistance alleles.

Keywords: wheat, molecular and genetic markers, resistance genes, stem rust.
Elucidation of the genetic mechanisms contributing to moenomycin resistance in actinobacteria
Authors: Ostash B. O. , Yushchuk O. S. , Koshla O. T. , Rebets Y. , Ostash I. S. , Sehin Y. V. , Busche T. , Kalinowski J. , Muth G. , Fedorenko V. O.
Published: March 2020

Aim. Moenomycins are phosphoglycolipid antibiotics produced almost exclusively by representatives of genus Streptomyces. These antibiotics directly inhibit peptidoglycan glycosyltransferases and are extremely active against cocci. Here we studied how antibiotic-producing actinobacteria protect themselves from toxic action of moenomycins. Methods. Microbiological and molecular genetic approaches were combined to reveal intrinsic levels and distribution of moenomycin resistance across actinobacteria genera, and to pinpoint genes contributing to moenomycin resistance in model strain Streptomyces coelicolor M145. Results. Out of 51 actinobacterial species (90 % of which Streptomyces) being tested, only Streptomyces albus J1074 turned out to be highly susceptible to moenomycin A, although resistant variants can be facilely raised. Several classes of mutations increased level of susceptibility of S. coelicolor to moenomycin, although in no case the latter was equal to what we observed in J1074 strain. Conclusions. Moenomycin resistance is widespread across actinobacteria, and it most likely is caused by a combination factors, such as richly decorated cell wall and organization of divisome apparatus. It is possible that moenomycin resistance mechanisms operating in actinobacteria and pathogenic cocci are different.

Keywords: moenomycin, antibiotic resistance, peptidoglycan.
The ability of maize Lancaster inbreds to callusogenesis in vitro under varying environmental conditions
Authors: Derkach K. V. , Borysova V. V. , Maletskyi V. O. , Satarova T. M.
Published: March 2020

Aim. This work is focused on the estimation of the callusogenic potential of 10 maize Lancaster germplasm inbreds in comparison with well-known inbreds-standards with high callusogenic ability A188, Chi31 and PLS61, and the identification of genotypes with stable morphogenic callus formation under varying conditions of donor plant cultivation during three years of researches. Methods. Method of cell, tissue and organ culture in vitro. Field method. One-way and two-way analysis of variance. Results. For the investigated Lancaster inbreds the average multi-annual value of the total frequency of callusogenesis was 80.1 %, the frequency of morphogenic type I callus formation was 25.7 %, type II callus formation was 43.8 %, but for inbreds-standards these ones reached, respectively, 96.2 %, 12.2 % and 65.4 %. The level of callusogenesis varied depending on the year of investigations for all studied genotypes. The influence of genotype, year conditions and the combination of these factors on callus induction for most of the studied inbreds was significant. Conclusions. The impact of the interaction of a genotype and ecological factors of donor plant cultivation on morphogenic callusogenesis of type I as well as type II was the most significant forLancaster inbreds.Lancaster inbreds ДK298, ДK6080, ДK212 and ДK420-1 were the most stable under varying environmental conditions on the total frequency of callusogenesis, the frequencies of morphogenic and type II callus formation. None of the studied inbreds revealed the stability on type I callus formation frequency. Keywords: maize (Zea mays L.), Lancaster germplasm, callusogenesis, culture in vitro.
Obtaining of transgenic potato plants (Solanum tuberosum L.) that contain antisense sequence of prolindehydrogenase gene
Authors: Ovcharenko O. O. , Rudas V. A. , Shcherbak N. L. , Kuchuk M. V.
Published: March 2020

Aim. The aim of our work was to obtain transgenic potato plants of Ukrainian varieties with the expression of a double-stranded RNA suppressor of proline dehydrogenase gene. We propose the decrease of proline degradation level and increase of overall proline concentration in obtained transgenic plants. Methods. The Agrobasterium tumefaciens-mediated method of genetic transformation to obtain transgenic plants of potato was used. Internodes of aseptic potato plants were transformed with a binary vector pBi2E containing an inverted repeats of two copies of proline dehydrogenase gene’s first exon and the gene of neomycin phosphotransferase II (nptII). Results. As a result of experiments kanamycin resistant transgenic potato lines of Deseiree, Belarusian 12 and Slavianka varieties were obtained. The transgenic nature of the obtained plants was confirmed by PCR with primers specific to the first exon of proline dehydrogenase and to nptII genes. Conclusions. The optimized conditions of genetic transformation and used agrobacterial strain allow to obtain the transgenic plants of a model potato variety Désirée, as well as varieties Belorussian 12 and Slovyanka which are of practical interest for cultivation in Ukraine. Keywords: transgenic plants, potatoes (Solanum tuberosum L.), stress resistance, proline.
The ploidy level of triticale plant regenerants, obtained by in vitro selection for resistance to abiotic stresses
Authors: Pykalo S. V. , Dubrovna O. V.
Published: March 2020

Aim. To analyze the ploidy level of plant regenerants of winter triticale, obtained by in vitro selection for resistance to osmotic and salt stresses. Methods. By cytological analysis and flow cytometry methods there was determined the ploidy level in the plant regenerants of winter triticale obtained by in vitro selection for resistance to abiotic stresses. Results. The somaclonal variability of plant regenerants of winter triticale resistant to osmotic and salt stresses by ploidy level was observed. The cytological instability of resistant’s regenerants was revealed that was due in appearance of aneuploidy plants. Plants with aneuploid chromosome set (38–41) were characterized by reduced viability and abnormal generative organs resulting they are not formed normal ears and not received seeds. Conclusions. Among the obtained regenerants euploids were in most cases indicating a selective advantage of hexaploid cells in ability to morphogenesis. Keywords: Triticale, plant regenerants, cytological analysis, aneuploids, abiotic stresses.
Association of TP53 Arg72Pro polymorphism (rs1042522) with bladder cancer risk in the Ukrainian population
Authors: Mishchuk Ya. M. , Kharkivska Ye. V. , Serga S. V. , Shklyar S. Ye. , Stakhovskyi O. E. , Stakhovskyi E. O. , Vitruk Yu. V. , Kononenko A. A. , Demydov S. V. , Ostapchenko L. I. , Kozeretska I. A.
Published: March 2020

Aim. To determine the frequency of TP53 polymorphic variants in bladder cancer patients and define possible association of this polymorphism with a bladder cancer in Ukrainians patients. Methods. The genotypes of TP53 gene at codon 72 were detected by PCR with allele specific primers. We investigated Arg72Pro polymorphism in 114 DNA samples of patients with bladder cancer. The PCR-amplified DNA products were subjected to electrophoresis in 3 % agarose. Results. The distribution of genotypes in group of patients with a bladder cancer was: Arg/Arg – 59.6 % (n=68), Arg/Pro – 40.4 % (n=46), Pro/Pro – 0 % (n=0). Genotype frequencies in patients (χ2=7.28, p=0.0007) weren’t in agreement with Hardy-Weinberg equilibrium. There were significant differences in the frequency of genotypes between the healthy individuals and the cancer group patients. Our result showed that patients with bladder cancer had a significantly higher frequency of Arg/Arg (F=42.7, p<0.05) and a lower frequency of Pro/Arg (F=40.9, p<0.05) compared to controls. Conclusions. Our study allows us to suggest that Arg/Arg polymorphic variant of TP53 is associated with the higher risk of bladder cancer development in the Ukrainian population (OR = 5,6, 95 % CI = 3.19 to 9.34, p < 0.0001). Keywords: TP53 gene, bladder cancer, polymorphism Arg72Pro, Ukrainian population.
Effect of short-term cold stress on flavonoid accumulation in Artemisia vulgaris and Artemisia annua “hairy” root cultures
Authors: Matvieieva N. A. , Havryliuk O. A. , Yastremska L. S.
Published: March 2020

Aim. The aim of the work was to investigate the effect of short-term cold stress (+10°С) on the growth, flavonoid synthesis and antioxidant activity in Artemisia vulgaris L. and A. annua L. “hairy“ root cultures. Methods. Transgenic roots were cultivated during the first 1, 2 and 5 days at +10°C on Murashige and Skoog basal medium with twice reduced macrosalt content. The total flavonoids content in Rutin equivalent was determined using alcohol extract reaction with alumunium chloride. Antioxidant activity was studied using the DPPH method. Results. Short-term cold stress resulted in a reduction of mass increment by 12–76 %. The total flavonoid content in «hairy» roots ranged from 32.0±3.13 to 187.0±21.04 mg RE/g dry weight. Decrease of temperature has led to decrease of the flavonoids content in No. 1, No. 2 A. vulgaris root lines and No. 5 A. annua line by 18–33 %. The reaction of No. 3 A. vulgaris and No. 4 A. annua root lines was expressed in stimulation flavonoid synthesis by 62 % and 56.5 %. Cultivation of «hairy» roots under short-term cold stress has led to decrease of the antioxidant activity in all roots lines by 4–40 %. Conclusions. Сold stress had negative effect the “hairy“ roots growth, stimulated flavonoids accumulation only in two “hairy” root lines and reduced the level of antioxidant activity.

Keywords: «hairy» roots culture, Artemisia spp., cold stress, flavonoids, antioxidant activity.
Biochemical characteristics of checkpea (Cicer arietinum L.) seeds for selection of genotypes of food direction
Authors: Molodchenkova O. O. , Kartuzova T. V. , Bezkrovnaya L. Ya. , Lykhota E. B. , Bushulyan O. V. , Lavrova G. D.
Published: March 2020

Aim. The goal of research was to study the features of biochemical composition  of the chickpea seed for detection the characteristics which can be used to genotype selection of food direction. Methods. Research methods are standard methodologies of plant biochemical analysis and methods developed in the laboratory. 7S and 11S globulins were separated by method, which was developed in the Laboratory of Plant Biochemistry (Patent # 42181, 107671). Results. The features of content of protein, fat, carbohydrates, isoflavones, activity of lectins, lipoxygenase, content and ratio of vicilin and legumin in the chickpea seed of genotypes of Ukrainian and foreign plant breeding were established. The genotype differences of component intensity, presence-absence in the electrophoretic spectrum of chickpea albumins, globulins, glutelins which effect on seed food value were evoluted using electrophoretic and densitometric analyses. Conclusions. The main biochemical characteristics of chickpea which connected with seed quality and food value, content and component composition of main protein fractions, presence in component composition of protein subunits that have negative influence on the man health can be used to selection of chickpea genotypes of food direction.

Keywords: сhickpea, breeding, vicilin, legumin, isoflavones.
Influence of the supplemental lightening by the solar-powered lamps on the growth, development and the grain productivity of the oat cultivar Nezlamny
Authors: Katsan V. A. , Potopalsky A. I. , Zadorozhnii B. O.
Published: March 2020

Aim. The article dedicated to the investigation of the influence of the supplemental lightening by the solar-powered lamps on the growth, development and the grain productivity of the oat cultivar Nezlamny during the cultivating in glass greenhouse under the short growing day conditions. Methods. The solar-powered lamps Expert Light ELWf-SGL-S11107 5P were used for the constant supplemental lightening. The lamps were exposed to the completion of the oat development. After the grain ripening, 10 plants in each variant were selected evenly of the plot area for analysis of the growth characteristics and the grain productivity elements. The next parameters were analyzed: the stem length (Ls), the main panicle length (L), the grains number in the panicle, (G); the grains weight from the panicle, (W). The obtained data were analyzed statistically. Results. Besides of the plants architecture alteration, the decrease of the grain productivity was shown in the prevailing majority plants affected by the constant supplemental solar-powered lamps lightening. Conclusions. The constant supplemental lightening by the solar-powered lamps may be the stressor negatively influenced the morphogenesis and the grain productivity of the oat plants. Keywords: supplemental lightening, solar-powered lamps, oat, changes of morphogenesis and productivity.
Inheritance of germination vigour in sugar beet sterility maintainers and their diallel hybrids
Authors: Kornieieva M. O. , Vakulenko P. I. , Andrieieva L. S. , Tymchyshyn S. M.
Published: March 2020

Aim. To determine the degree of dominance of the “seed germination” sign and combination ability of sterility maintainers using diallel analysis. Methods. Hybridization of sterility maintainers was arranged as diallel crosses. Genetic determination of the sign was determined in accordance with Heyman’s method. Results. Used in crossing sterility maintainer lines have been studied in terms of their productivity in previous variety tests. D. The dominant inheritance of “seed germination” sign in diallel hybrids was determined as following: in the structure of the sign variability 43 % of cross combinations inherited this sign as positive dominance. Genetic determination of the “synergy of germination” sign was determined by the effects of combination ability. The additive effect of the female parent was assessed at 18.4 %, in male parent at 18.1 %, while the nonadditive effect of interaction between the components was at 40.7 % The influence of cytoplasmic effects amounted to 22,7 %. Selected were the best lines in specific pairs; their genetic value was studied as the average value of heterosis in many crossings involving specific lines. Conclusions. When forming sources of improved signs it is advisable to consider the cytoplasmic gene effects, which share is estimated at 22.7 %. The best lines in terms of general combination ability for seed germination found to be sterility maintainers Ot 1 and Ot 2.

Keywords: sterility maintainers, combination ability, seed germination, additive, non-additive, and reciprocal effects.
Impact of ASCO/CAP 2007, 2013 and 2018 recommendations on Her-2/neu gene amplification status testing in patients with breast cancer
Authors: Klimuk B. T. , Duhan O. M. , Polinyk S. I. , Rybchenko L. A. , Klymenko S. V.
Published: March 2020

Aim. To Assess the significance of changes in the diagnostics of Her-2/neu gene amplification status using the ASCO/CAP 2007, 2013 and 2018 recommendations by retrospective evaluation of breast cancer (BC) tissue test results performed by fluorescence in situ hybridization (FISH). Methods. Her-2/neu FISH dual probe test data of 797 BC specimens were re-evaluated in accordance with the requirements of the consecutive updated ASCO/CAP recommendations. Results. The proportion of cases without Her-2/neu gene amplification by the ASCO/CAP 2013 and 2018 criteria was 11,0 % lower than by the 2007 ASCO/CAP criteria. There was no statistically significant difference between the number of Her-2/neu gene amplification positive cases according to ASCO/CAP 2007, 2013 and 2018 criteria. The proportion of cases with a doubtful outcome for the use of ASCO/CAP in 2018 was significantly higher (12,2 %, p < 0,01) compared to that by ASCO/CAP 2007 and 2013 (6,6 % and 12,2 % accordingly). Conclusions. The findings of a re-benchmarking review (in accordance with the 2018 ASCO/CAP criteria) of the FISH case study results show practical differences from the previous ASCO/CAP 2007, 2013 recommendations. The proportion of samples, which needs the additional research, increases with each revision of the recommendations against the backdrop of the expansion of the case group with questionable amplification status of the Her-2/neu gene by the FISH double probe method. Keywords: ASCO/CAP, amplification status of the gene Her-2/neu, breast cancer.
The effect of aseptic cultivation of Crambe mitridatis Juz. plants on its biochemical composition
Authors: Pushkarova N. O. , Kyrpa-Nesmiian T. M. , Kuchuk M. V.
Published: March 2020

The aim of the research was to establish efficient microclonal propagation conditions of endangered Crambe mitridatis plants in vitro and to study the possible effect of aseptic cultivation on biochemical composition (hydroxycinnamic acids, flavonoids, phenolic compounds) of plants. Methods. In vitro plant culture methods were applied. Seeds were used for aseptic culture initiation. Morphogenic potential of root, leaf and petiole explants was studied on Murashige-Skoog medium with addition of plant growth regulators. The content of biologically active compounds was measured using spectrometry in plants grown in aseptic conditions and in the greenhouse. Results. Morphogenic potential of root, leaf and petiole explants was studied and the highest regeneration frequency of plantlets was established for root explants (80 %), for petiole explants (50 %) and the lowest for leaf explants (20 %). It was found that plants cultivated in aseptic conditions have higher hydroxycinnamic acids, flavonoids and phenolic compounds compared to plants grown in vivo. Conclusions. It is advisable to multiply C. mitridatis plants in vitro via root and petiole explants. Aseptic cultivation contributes to synthesis of biologically active compounds (auxin synergists) in C. mitridatis plants.Keywords: in vitro culture, hydroxycinnamic acids, flavonoids, phenolic compounds, Crambe mіtridatis.
"Værdifortolkning" og "værdibedømmelse" hos Weber og Durkheim
Authors: Harste Gorm
Published: March 2020

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L’ACCÈS À L’ASSAINISSEMENT DANS LES QUARTIERS PRÉCAIRES DE OUAGADOUGOU
Authors: Laré Amandine , Briand Anne , Kéré Eric N.
Published: March 2020

La littérature a très peu étudié la demande en assainissement amélioré des ménages et notamment en Afrique. Seules quelques rares études ont été menées mais dans le cadre d’évaluation contingente (demande ex ante). À partir de modèles de choix discrets, nous analysons les déterminants de la demande avérée (ex post) pour des services améliorés en assainissement dans six quartiers précaires de Ouagadougou. Si certains résultats corroborent ceux de la littérature, d’autres s’avérant bien plus originaux, ont été mis en évidence. La contribution la plus originale de ce travail porte sur l’établissement d’un lien négatif contre-intuitif entre l’accès amélioré à l’assainissement et la prévalence des maladies hydriques. L’usage d’une latrine améliorée ne suffirait pas à échapper aux maladies hydriques. Ces résultats suggèrent donc qu’une combinaison d’interventions publiques est nécessaire; les différentes cibles des OMD (en particulier, l’amélioration de l’accès à l’eau potable, des services en assainissement et la réduction de la prévalence des maladies hydriques) étant intimement liées.
LESSONS FROM A COSTS ANALYSIS IN POTATO PRODUCTION
Authors: Marcomini Gilson Rogério , Patino Marco Tulio Ospina , Alam Mohammad Jahangir , Amorim Fernando Rodrigues de
Published: March 2020

The potato is one of the most widely cultivated horticultural crops in the world. Brazil ranks 21st among the world's largest potato producers, and the United States (US) is the fifth largest producer in the world. This study analyzes and compares the economic, financial aspects of potato production in the Vargem Grande do Sul region, SP (Brazil), and southwestern Idaho (US), seeking to identify which aspects of American production can serve as reference for technical and financial improvement of the Brazilian production. Data from official sources were analyzed using principal components analysis (PCA). Potato producer in Vargem Grande do Sul need learning how to reduce the factors of production costs to have better financial results in your potato production.
Levels and stages of artificial defoliation in the agronomic performance of the cassava crop
Authors: Ecco Martios , Da Costa Antônio Carlos Torres , Júnior José Barbosa Duarte , Borsoi Augustinho , Arrúa Milciades Ariel Melgarejo
Published: March 2020

The cassava crop is susceptible to foliar loss due to several pests, which interfere with the photosynthetic rate, causing large losses mainly to its organ of interest, which are the roots. The objective of this study was to evaluate the agronomic performance of cassava crop following different levels of artificial defoliation at different stages of development. The experiment was conducted in Guaíra-PR, using a randomized block design in a 5 × 5 factorial scheme, with five levels of defoliation in five developmental stages. The levels consisted of 0, 25, 50, 75, and 100% defoliation, and the stages were 45, 90, 135, 180, and 225 days after planting (DAP). The responses of the variable height and plant diameter were inversely proportional according to the interaction of the factors levels and times of defoliation, especially when 100% defoliation was performed at 90 DAP. For root weight, the greatest level of defoliation at 132 DAP resulted in a low yield, whereas for starch deposition in the roots, only the levels had a significant effect for reduction, presenting a linear decreasing effect according to an increase in defoliation. In conclusion, high levels of defoliation between 122 and 132 DAP resulted in greater damage to the root mass; these high defoliation levels independent of the defoliation stage caused the largest losses in the amount of stored starch.
Chemical composition and biological properties of Pleurotus spp. cultivated on peat moss and wheat straw
Authors: Valenzuela-Cobos Juan Diego , Rodríguez-Grimón René Oscar , Zied Diego Cunha , Endara Ana Grijalva- , Garcés-Moncayo María Fernanda , Garín-Aguilar María Eugenia , Sánchez-Hernández Abraham , Valencia del Toro Gustavo
Published: March 2020

The productivity parameters of 2 Pleurotus spp. cultivated with two mixtures of wheat straw (WS) and peat moss (MG) were evaluated, the chemical composition of the mushrooms and also the antioxidant, antibacterial activities of hexanic extracts of the fruit bodies were determined. Pleurotus strains were fructified using the mixture 1 (60% of WS + 40% of MG) obtained the highest biological efficiencies, production rates and yields. The strain of Pleurotus ostreatus (XC005) cultivated using the mixture 2 (80% WS + 20% of MG) presented fruit bodies with highest protein content being of 25.28%, and also the strain of Pleurotus djamor (XC007) fructified on the mixture 2 showed mushrooms with highest protein content being of 30.98%. The highest antioxidant effect resulted in the hexanic extract of the Pleurotus ostreatus cultivated on M2 was found using the TBARS inhibition assay (EC50 = 0.12 mg mL- 1), while the highest antioxidant activity showed the hexanic extract of the Pleurotus djamor produced on M1 was found using the TBARS inhibition assay (EC50 = 0.21 mg mL- 1). The bactericidal effect of the hexanic extracts of Pleurotus ostreatus was obtained between 21.09 to 27.90 mg mL−1, whereas the bacteriostatic activity of the hexanic extracts of Pleurotus djamor was achieved since 19.82 to 30.98 mg mL−1. The results evidenced that the Pleurotus spp. cultivated on the mixture 2 presented highest protein content and better biological properties, encouraging the production of edible fungi strains and their use in the industrial field.