bluuebunny/crossref_metadata_2025 huggingface dataset:
Do Fire Regime Attributes Affect Soil Biochemical Properties in the Same Way under Different Environmental Conditions?
Authors: Fernández-García Víctor , Marcos Elena , Reyes Otilia , Calvo Leonor
Published: February 2020

Global change is altering fire frequency and severity in many regions across the world. In this work, we studied the impact of different frequency and severity regimes on the soil biochemical properties in burned areas with different environmental conditions. We selected three sites dominated by pine ecosystems along a Mediterranean-Transition-Oceanic climatic gradient, where we determined the fire frequency, and severity of the last wildfire. Four years after the last wildfire, we established 184 4 m2 plots. In each plot, we collected a composed soil sample from a 3 cm depth, and measured several ecological variables potentially affected by the fire frequency and severity (cover of bare soil, cover of fine and coarse plant debris, cover of vegetation, and vegetation height). From each soil sample, we analyzed the enzymatic activities corresponding to the biogeochemical cycles of carbon, nitrogen, and phosphorus (β-glucosidase, urease, and acid-phosphatase, respectively), and the microbial biomass carbon. The results indicated that fire frequency only played a significant role in soil biochemical properties at the Mediterranean and Transition sites. Specifically, we found that increases in frequency contributed to increased urease and phosphatase activities (at the Transition site), as well as microbial biomass carbon (at the Mediterranean and Transition sites). In relation to burn severity, we found opposite patterns when comparing the Mediterranean and Oceanic sites. Specifically, increased severity significantly decreased β-glucosidase, urease, and microbial biomass carbon at the Mediterranean site, whereas at the Oceanic one, severity significantly increased them. Burn severity also decreased microbial biomass carbon at the Transition site. Our results also indicated that, overall, fire frequency determined the studied ecological variables at the Mediterranean and Transition sites, but clear indirect effects on biochemical properties due to changes in ecological variables were not found. This study adds to the knowledge on the impact of shifts in fire regimes on soils in the current context of change.
Analysis and Laboratory Testing of Technical Injury Prevention Measures for Portable Combustion Chainsaws
Authors: Dąbrowski Andrzej
Published: February 2020

Portable chainsaws for cutting wood, like other handheld machines, are commonly used for mechanical work. Portable combustion chainsaws are still used in many countries, including Poland, as basic wood harvesting machines and in agriculture. The use of portable chainsaws, however, poses a high risk of injury, especially with the phenomenon of kickback. Important European documents (e.g., 2006/42/EC) qualify these machines as a specific group, and assessing them for compliance with the essential applicable requirements (before placing them on the market) can be done with the participation of a third party (notified body). In Poland, only employees with appropriate training are allowed to work with chainsaws for professional wood harvesting. This paper presents the results of an analysis of the hazards posed by portable combustion chainsaws. A practical assessment was conducted of the effectiveness of the most relevant technical solutions used to protect operators from injury. Reference is made to tests on chainsaws that were carried out as part of scientific research (described in previous publications) and as part of research commissioned by market surveillance authorities operating in Poland. The presented research results show that the risk of injury caused by the use of chainsaws is influenced not only by the design features of the machines but also by their proper use, including the selection of equipment appropriate to the working conditions (e.g., wood type and properties, use of chainsaws for professional or non-professional work).
Three Invasive Tree Species Change Soil Chemistry in Guam Forests
Authors: Marler Thomas E.
Published: February 2020

Research Highlights: Established stands of Leucaena leucocephala (Lam.) de Wit, Spathodea campanulata P. Beauv., and Vitex parviflora Juss. modified soils in Guam’s limestone forests, reducing storage pools of carbon, nitrogen, and phosphorus. Background and Objectives: Invasive plants may engineer negative changes in ecosystem properties. This study was conducted to determine changes in soil chemistry following infestations of three problematic tree species on Guam. Materials and Methods: Minerals, metals, and mineralization dynamics were measured in invaded sites and paired sites with biodiverse native tree cover. Results: Most soil properties were significantly changed by long-term infestations of the invasive tree species. The soils within invaded sites exhibited total carbon, total nitrogen, and available phosphorus that were less than native sites. In contrast, the carbon/nitrogen ratio increased for every species-site combination. The other chemical properties were idiosyncratic among the sites and species. Conclusions: Mitigation and restoration activities that include the removal of these trees from project sites may require many years for the below-ground ecosystems to return to their native state. These three invasive trees decrease the ability of Guam soils to sequester recalcitrant forms of carbon, nitrogen, and phosphorus.
Modelling the Crown Profile of Western Hemlock (Tsuga heterophylla) with a Combination of Component and Aggregate Measures of Crown Size
Authors: Cameron Ian R. , Parish Roberta , Goudie James W. , Bealle Statland Catherine A.
Published: February 2020

Research Highlights: We present statistical methods for using crown measurement data from multiple destructive sampling studies to model crown profiles in the Tree and Stand Simulator (TASS) and evaluate it using component (branch-level) and aggregate (tree-level) predictions. Combining data collected under different sampling protocols offered unique challenges. Background and Objectives: The approach to modelling crown profiles was based on Mitchell’s monograph on Douglas-fir growth and simulated dynamics. The functional form defines the potential crown size and shape and governs the rate of crown expansion. With the availability of additional data, we are able to update these functions as part of ongoing TASS development and demonstrate the formulation and fitting of new crown profile equations for stand-grown western hemlock (Tsuga heterophylla (Raf.) Sarg. Materials and Methods: Detailed measurements on 1616 branches from 153 trees were collected for TASS development over a 40-year period. Data were collected under two different sampling protocols and the methods were designed to allow the use of data from both protocols. Data collected on all branches were then introduced through the application of the ratio of length of each of the selected branches to the largest branch in the internode (RL). Results: A mixed-effects model with two random effects, which accounted for tree-level variation, provided the best fit. From that, a model that expressed one parameter as a function of another with one random effect was developed to complement the structure of the Tree and Stand Simulator (TASS). The models generally over-estimated crown size when compared to the projected crown area recorded from field measurements, and a scalar adjustment factor of 0.89 was applied that minimised mean-squared error of the differences. The new model is fit from direct measures of crown radius and predicts narrower crown shapes than previous functions used in TASS.
The Geriatric Surgery: The Importance of Frailty Identification Beyond Chronological Age
Authors: Boccardi Virginia , Marano Luigi
Published: February 2020

In the last fifty years, there has been a great improvement in social and health conditions [...]
Predictive Factors of Deep Vein Thrombosis in Gynecologic Cancer Survivors with Lower Extremity Edema: A Single-Center and Retrospective Study
Authors: Kim Jungin , Kim Hyun-Jun , Park Seunghun , Kim Dong Kyu , Kim Tae Hee
Published: February 2020

This study was conducted to examine predictive factors of deep vein thrombosis (DVT) in gynecologic cancer survivors with lower extremity edema (LEE). In the current single-center, retrospective study, there was a total of 315 eligible patients, including 80 patients with DVT and 235 without DVT. They were therefore divided into two groups: the DVT group (n = 80) and the non-DVT group (n = 235). Then, baseline and clinical characteristics of the patients were compared between the two groups. In our study, distant organ metastasis, advanced stage, lymphadectomy, and amount of intraoperative blood loss had a positive predictive value for the occurrence of DVT in gynecologic cancer survivors presenting LEE. In conclusion, our results indicate that it is necessary to consider the possibility of LEE arising from DVT in gynecologic cancer survivors with advanced-stage cancer, distant organ metastasis, lymphadectomy, and intraoperative blood loss over 1500 mL.
Volatile Fragility: New Employment Forms and Disrupted Employment Protection in the New Economy
Authors: Chen Bin , Liu Tao , Wang Yingqi
Published: February 2020

This research is based on empirical surveys conducted in two Chinese cities, Beijing and Chengdu, which examine employment relationships, labor protection and social protection in the new digital economy. Through these theoretically informed surveys on various forms of employment via online platforms, we have found that the organizational principles and functional patterns of employment have profoundly transformed in the epoch of digitalization. The traditional employment relationship characterized by written contracts with clearly defined entitlements and obligations for employers and employees have been increasingly substituted by new volatile, fluid and fragile employment forms, softening the labor rights and social rights of “digital employees” and strengthening social control over them through online evaluation systems supported by smart phones and apps. The employees engaged in the online sharing economy have become more individualized and atomized than ever before, resulting in the emergence of an unorganized and disenfranchised “digital working class”.
Altered Cerebral Blood Flow and Potential Neuroprotective Effect of Human Relaxin-2 (Serelaxin) During Hypoxia or Severe Hypovolemia in a Sheep Model
Authors: Schiffner René , Bischoff Sabine J. , Lehmann Thomas , Irintchev Andrey , Nistor Marius , Lemke Cornelius , Schmidt Martin
Published: February 2020

Specific neuroprotective strategies to minimize cerebral damage caused by severe hypoxia or hypovolemia are lacking. Based on previous studies showing that relaxin-2/serelaxin increases cortical cerebral blood flow, we postulated that serelaxin might provide a neuroprotective effect. Therefore, we tested serelaxin in two emergency models: hypoxia was induced via inhalation of 5% oxygen and 95% nitrogen for 12 min; thereafter, the animals were reoxygenated. Hypovolemia was induced and maintained for 20 min by removal of 50% of the total blood volume; thereafter, the animals were retransfused. In each damage model, the serelaxin group received an intravenous injection of 30 µg/kg of serelaxin in saline, while control animals received saline only. Blood gases, shock index values, heart frequency, blood pressure, and renal blood flow showed almost no significant differences between control and treatment groups in both settings. However, serelaxin significantly blunted the increase of lactate during hypovolemia. Serelaxin treatment resulted in significantly elevated cortical cerebral blood flow (CBF) in both damage models, compared with the respective control groups. Measurements of the neuroproteins S100B and neuron-specific enolase in cerebrospinal fluid revealed a neuroprotective effect of serelaxin treatment in both hypoxic and hypovolemic animals, whereas in control animals, neuroproteins increased during the experiment. Western blotting showed the expression of relaxin receptors and indicated region-specific differences in relaxin receptor-mediated signaling in cortical and subcortical brain arterioles, respectively. Our findings support the hypothesis that serelaxin is a potential neuroprotectant during hypoxia and hypovolemia. Due to its preferential improvement of cortical CBF, serelaxin might reduce cognitive impairments associated with these emergencies.
Smali+: An Operational Semantics for Low-Level Code Generated from Reverse Engineering Android Applications
Authors: Ziadia Marwa , Fattahi Jaouhar , Mejri Mohamed , Pricop Emil
Published: February 2020

Today, Android accounts for more than 80% of the global market share. Such a high rate makes Android applications an important topic that raises serious questions about its security, privacy, misbehavior and correctness. Application code analysis is obviously the most appropriate and natural means to address these issues. However, no analysis could be led with confidence in the absence of a solid formal foundation. In this paper, we propose a full-fledged formal approach to build the operational semantics of a given Android application by reverse-engineering its assembler-type code, called Smali. We call the new formal language Smali + . Its semantics consist of two parts. The first one models a single-threaded program, in which a set of main instructions is presented. The second one presents the semantics of a multi-threaded program which is an important feature in Android that has been glossed over in the-state-of-the-art works. All multi-threading essentials such as scheduling, threads communication and synchronization are considered in these semantics. The resulting semantics, forming Smali + , are intended to provide a formal basis for developing security enforcement, analysis and misbehaving detection techniques for Android applications.
Development of Control Experiments for an Online Laboratory System
Authors: Rábek Matej , Žáková Katarína
Published: February 2020

Remote experiments have been gaining a lot of popularity over the last years. They are available for many areas including control education. The majority of laboratories available via the Internet were developed from scratch and lack modularity, which enables their easier adaptation. Crucially, the diversity of experiments that can be performed on a single device is quite limited. Along with the prospect of simple integration of new devices and simulation environments, this approach presents a way to more effectively utilize the available resources. The presented online laboratory system offers a possibility of an easy integration of new control experiments to the online environment. It allows users to define selected variables inside their block diagrams, upload them to the system and later initialize them within the system’s graphical user interface. The system was tested on a new developed air levitation plant that can be controlled via Matlab simulation environment.
Relish as a Candidate Marker for Transgenerational Immune Priming in a Dampwood Termite (Blattodae: Archeotermopsidae)
Authors: Cole Erin L. , Empringham Jessica S. , Biro Colette , Thompson Graham J. , Rosengaus Rebeca B.
Published: February 2020

Natural selection should favor the transfer of immune competence from one generation to the next in a context-dependent manner. Transgenerational immune priming (TGIP) is expected to evolve when species exploit pathogen-rich environments and exhibit extended overlap of parent–offspring generations. Dampwood termites are hemimetabolous, eusocial insects (Blattodea: Archeotermopsidae) that possess both of these traits. We predict that offspring of pathogen-exposed queens of Zootermopsis angusticollis will show evidence of a primed immune system relative to the offspring of unexposed controls. We found that Relish transcripts, one of two immune marker loci tested, were enhanced in two-day-old embryos when laid by Serratia-injected queens. These data implicate the immune deficiency (IMD) signaling pathway in TGIP. Although an independent antibacterial assay revealed that embryos do express antibacterial properties, these do not vary as a function of parental treatment. Taken together, Z. angusticollis shows transcriptional but not translational evidence for TGIP. This apparent incongruence between the transcriptional and antimicrobial response from termites suggests that effectors are either absent in two-day-old embryos or their activity is too subtle to detect with our antibacterial assay. In total, we provide the first suggestive evidence of transgenerational immune priming in a termite.
Liver Bioreactor Design Issues of Fluid Flow and Zonation, Fibrosis, and Mechanics: A Computational Perspective
Authors: Rezania Vahid , Coombe Dennis , Tuszynski Jack
Published: February 2020

Tissue engineering, with the goal of repairing or replacing damaged tissue and organs, has continued to make dramatic science-based advances since its origins in the late 1980’s and early 1990’s. Such advances are always multi-disciplinary in nature, from basic biology and chemistry through physics and mathematics to various engineering and computer fields. This review will focus its attention on two topics critical for tissue engineering liver development: (a) fluid flow, zonation, and drug screening, and (b) biomechanics, tissue stiffness, and fibrosis, all within the context of 3D structures. First, a general overview of various bioreactor designs developed to investigate fluid transport and tissue biomechanics is given. This includes a mention of computational fluid dynamic methods used to optimize and validate these designs. Thereafter, the perspective provided by computer simulations of flow, reactive transport, and biomechanics responses at the scale of the liver lobule and liver tissue is outlined, in addition to how bioreactor-measured properties can be utilized in these models. Here, the fundamental issues of tortuosity and upscaling are highlighted, as well as the role of disease and fibrosis in these issues. Some idealized simulations of the effects of fibrosis on lobule drug transport and mechanics responses are provided to further illustrate these concepts. This review concludes with an outline of some practical applications of tissue engineering advances and how efficient computational upscaling techniques, such as dual continuum modeling, might be used to quantify the transition of bioreactor results to the full liver scale.
A Research on the Classification and Applicability of the Mobile Health Applications
Authors: Pires Ivan Miguel , Marques Gonçalo , Garcia Nuno M. , Flórez-Revuelta Francisco , Ponciano Vasco , Oniani Salome
Published: February 2020

Mobile health applications are applied for different purposes. Healthcare professionals and other users can use this type of mobile applications for specific tasks, such as diagnosis, information, prevention, treatment, and communication. This paper presents an analysis of mobile health applications used by healthcare professionals and their patients. A secondary objective of this article is to evaluate the scientific validation of these mobile health applications and to verify if the results provided by these applications have an underlying sound scientific foundation. This study also analyzed literature references and the use of mobile health applications available in online application stores. In general, a large part of these mobile health applications provides information about scientific validation. However, some mobile health applications are not validated. Therefore, the main contribution of this paper is to provide a comprehensive analysis of the usability and user-perceived quality of mobile health applications and the challenges related to scientific validation of these mobile applications.
Study on Lubrication Characteristics of Metal Liquid Film Based on Electromagnetic-Elastic Mechanics-Hydrodynamics Multiphysics Coupling Model
Authors: Li Chengxian , Xia Shengguo
Published: February 2020

In an electromagnetic rail launcher, a metal liquid film is created at the armature/rail (A/R) contact interface. It has a significant impact on electromagnetic launch performance. In this paper, an electromagnetic-elastic mechanics-hydrodynamics multi physics coupling model is established in consideration of the metal liquid film’s own acceleration, magnetic pressure and dynamic changes in film thickness. Based on this model, the lubricating characteristics of magnetic pressure and fluid pressure distribution, film thickness distribution and velocity distribution of the metal liquid film were studied. When the velocity of the metal liquid film is very fast, and the magnetic pressure is reduced, it may fail to maintain stability and rupture, which may be an important reason for the transition. Finally, this paper analyzes the lubrication effect of the metal liquid film, and points out that when we want strictly to control the muzzle velocity, the lubrication effect of the metal liquid film must be considered.
Inhibition of Virus-Induced Cytokine Production from Airway Epithelial Cells by the Late Addition of Budesonide
Authors: Homma Tetsuya , Fukuda Yosuke , Uchida Yoshitaka , Uno Tomoki , Jinno Megumi , Kishino Yasunari , Yamamoto Mayumi , Sato Hiroki , Akimoto Kaho , Kaneko Keisuke , Fujiwara Akiko , Sato Haruna , Hirai Kuniaki , Miyata Yoshito , Inoue Hideki , Ohta Shin , Watanabe Yoshio , Kusumoto Sojiro , Ando Koichi , Suzuki Shintaro , Yamaoka Toshimitsu , Tanaka Akihiko , Ohmori Tohru , Sagara Hironori
Published: February 2020

Background and objectives: Viral infection is the main cause of asthma and COPD (chronic obstructive pulmonary disease) exacerbation and accumulate inflammatory cells to airway tissue. We have reported poly I:C, a mimic product of the virus and ligand of toll-like receptor 3 (TLR3), induced inflammatory chemokines from airway epithelial cells and found prior incubation with corticosteroids diminishes the effect of TLR3 activation. In clinical practice, mild asthma is recommended as-needed budesonide (BUD) when symptoms occur following a viral infection, etc. However, many questions still surround BUD’s usefulness if taken after a virus has already infected airway tissue. The aim of this study was to investigate the inhibitory effects of BUD on inflammatory cytokines induced by viral infection. Materials and Methods: Normal human bronchial epithelial (NHBE) cells were stimulated with poly I:C or infected with human rhinovirus-16 (HRV16) and BUD was added after the initial stimulation. Expression of both thymic stromal lymphopoietin (TSLP) and CCL26/eotaxin-3 was quantified by real-time RT-PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Knockdown study was performed. Results: Pre-or post-incubation with BUD inhibited both poly I:C- and HRV16-induced mRNAs and proteins of both thymic stromal lymphopoietin (TSLP) and CCL26 with significance. Knockdown of the glucocorticoid receptor diminished these effects of BUD. Under the same conditions of BUD’s experiment, post-incubation with neither fluticasone propionate nor dexamethasone suppressed expression of both TSLP and CCL26, which induced by poly I:C. Conclusion: Post-addition of BUD inhibited the virus-induced TSLP and CCL26 from the airway epithelial cells. These results suggest that inhalation of BUD after viral infection has beneficial effects on asthma. Conclusion: Late addition of BUD may benefit among patient with viral infection and type 2 allergic airway disease such as asthma.
The Relationship Between Contrast Associated Nephropathy and Coronary Collateral Circulation in very Old Patients
Authors: Yildirim Tarik , Ozkan Birol , Alici Gokhan , Yildirim Seda Elcim , Bugra Onursal , Kadi Hasan
Published: February 2020

Background: The aim of this study was to investigate whether there is a relationship between coronary collateral circulation (CCC) and contrast associated nephropathy (CAN) in very elderly patients. Methods: Patients aged 90 years or older with at least one major occlusion of the coronary artery proximal or mid-section were included in the study. CCC was graded according to the Rentrop classification. CAN was defined as an increase in blood creatinine value of 25% or more on the second day after coronary angiography. Results: Thirty-six patients who met the study criteria were included in the study. In the study group, CAN developed in 12 patients (CAN (+) group), 24 patients did not develop CAN (CAN (−) group). The creatinine levels before coronary angiography were 1.05 ± 0.12 in the CAN (−) group and 1.22 ± 0.14 in the CAN (+) group. Baseline creatinine values were significantly higher in the CAN (+) group (p = 0.001). The contrast agent used in the CAN (+) group was significantly higher (p = 0.001). In the CAN (+) group, nine patients (43%) had poor collateral circulation, whereas only three patients (20%) had well-developed collateral circulation. In a logistic regression analysis, the collateral class was not a risk factor for CAN, whereas contrast agent volume and basal creatinine were independent predictors of CAN. Conclusion: We found that CCC grade was not associated with the development of CAN in very old patients, but the amount of contrast agent and pre-procedure creatinine values were independent variables in the development of CAN.
Revealing Sources of Variation for Reproducible Imaging of Protein Assemblies by Electron Microscopy
Authors: Kepiro Ibolya E. , Nardone Brunello , Page Anton , Ryadnov Maxim G
Published: February 2020

Electron microscopy plays an important role in the analysis of functional nano-to-microstructures. Substrates and staining procedures present common sources of variation for the analysis. However, systematic investigations on the impact of these sources on data interpretation are lacking. Here we pinpoint key determinants associated with reproducibility issues in the imaging of archetypal protein assemblies, protein shells, and filaments. The effect of staining on the morphological characteristics of the assemblies was assessed to reveal differential features for anisotropic (filaments) and isotropic (shells) forms. Commercial substrates and coatings under the same staining conditions gave comparable results for the same model assembly, while highlighting intrinsic sample variations including the density and heterogenous distribution of assemblies on the substrate surface. With no aberrant or disrupted structures observed, and putative artefacts limited to substrate-associated markings, the study emphasizes that reproducible imaging must correlate with an optimal combination of substrate stability, stain homogeneity, accelerating voltage, and magnification.
Enhanced Anaerobic Digestion of Long Chain Fatty Acid by Adding Magnetite and Carbon Nanotubes
Authors: Mostafa Alsayed , Im Seongwon , Song Young-Chae , Kang Seoktae , Kim Dong-Hoon
Published: February 2020

This study investigated the impact of stimulating direct interspecies electron transfer (DIET), by supplementing nano-sized magnetite (nFe3O4, 0.5 g Fe/g VSS) and carbon nanotubes (CNT, 1 g/L), in anaerobic digestion of oleic acid (OA) at various concentrations (0.10–4.00 g chemical oxygen demand(COD)/L). Both supplementations could enhance CH4 production, and its beneficial impact increased with increased OA concentration. The biggest improvements of 114% and 165% compared to the control were achieved by nFe3O4 and CNT, respectively, at OA of 4 g COD/L. The enhancement can be attributed to the increased sludge conductivity: 7.1 ± 0.5 (control), 12.5 ± 0.8 (nFe3O4-added), and 15.7 ± 1.1 µS/cm (CNT-supplemented). Dissolved iron concentration, released from nFe3O4, seemed to have a negligible role in improving CH4 production. The excretion of electron shuttles, i.e., humic-like substances and protein-like substances, were found to be stimulated by supplementing nFe3O4 and CNT. Microbial diversity was found to be simplified under DIET-stimulating conditions, whereby five genera accounted for 88% of the total sequences in the control, while more than 82% were represented by only two genera (Methanotrix concilli and Methanosarcina flavescens) by supplementing nFe3O4 and CNT. In addition, the abudance of electro-active bacteria such as Syntrophomonas zehnderi was significantly increased from 17% to around 45%.
Digestive Ability, Physiological Characteristics, and Rumen Bacterial Community of Holstein Finishing Steers in Response to Three Nutrient Density Diets as Fattening Phases Advanced
Authors: Qiu Qinghua , Gao Chaoyu , Aziz ur Rahman Muhammad , Cao Binghai , Su Huawei
Published: February 2020

The aim of this study is to track the dynamic alterations in nutrient intake and digestion, rumen fermentation and plasma metabolic characteristics, and rumen bacterial community of Holstein finishing steers in response to three nutrient density diets as fattening phases advanced. A total of eighteen Holstein steers were randomly allocated into three nutrient density groups and steers in each group were fed under a three-phase fattening strategy, with nutrient density increased in each group when fattening phase advanced. Results showed that both fattening phase and dietary nutrient density significantly influenced the nutrient digestion, most of the rumen fermentation parameters, and part of bacteria at phylum and genus levels. Individually, dietary nutrient density affected the concentrations of plasma alanine aminotransferase and urea N, bacterial richness and evenness. All determined nutrient intake and plasma biochemical parameters, except for alanine aminotransferase and triglyceride, differed among fattening phases. Spearman correlation analysis revealed strong correlations between fiber intake and bacterial richness and evenness, rumen fermentation characteristics and certain bacteria. Moreover, Patescibacteria abundance was positively correlated with ambient temperature and plasma total protein. These results indicate that rumen fermentation and nutrient digestion were influenced by both dietary nutrient density and fattening phase, and these influences were regulated by certain rumen bacterial community and ruminal bacteria may be affected simultaneously by ambient temperature. This study may provide insights into diet optimization and potentially adaptive mechanism of rumen bacterial community in response to fattening phases and gradually climatic change.
Thermoelectric Properties of NiCl3 Monolayer: A First-Principles-Based Transport Study
Authors: Liu Jing , Chen Xiaorui , Huang Yuhong , Yuan Hongkuan , Chen Hong
Published: February 2020

By employing the first-principles-based transport theory, we investigate the thermoelectric performance based on the structural and electronic properties of NiCl 3 monolayer. The NiCl 3 monolayer is confirmed to be a stable Dirac spin gapless semiconductor with the linear energy dispersion having almost massless carrier, high carrier mobility and fully spin-polarization. Further, NiCl 3 monolayer processes the optimum power factor of 4.97 mWm − 1 K − 2 , the lattice thermal conductivity of 1.89 Wm − 1 K − 1 , and the dimensionless figure of merit of 0.44 at room temperature under reasonable carrier concentration, indicating that NiCl 3 monolayer may be a potential matrix for promising thermoelectrics.
Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
Authors: Brandão Fátima , Fernández-Bertólez Natalia , Rosário Fernanda , Bessa Maria João , Fraga Sónia , Pásaro Eduardo , Teixeira João Paulo , Laffon Blanca , Valdiglesias Vanessa , Costa Carla
Published: February 2020

Titanium dioxide nanoparticles (TiO2 NPs) have a wide variety of applications in many consumer products, including as food additives, increasing the concern about the possible hazards that TiO2 NPs may pose to human health. Although most previous studies have focused on the respiratory system, ingestion must also be considered as an important exposure route. Furthermore, after inhalation or ingestion, TiO2 NPs can reach several organs, such as the liver, brain or lungs. Taking this into consideration, the present study focuses on the uptake and potential genotoxicity (micronuclei induction) of TiO2 NPs on four human cell lines of diverse origin: lung cells (A549), liver cells (HepG2), glial cells (A172) and neurons (SH-SY5Y), using flow cytometry methods. Results showed a concentration-, time- and cell-type- dependent increase in TiO2 NPs uptake but no significant induction of micronuclei in any of the tested conditions. Data obtained reinforce the importance of cell model and testing protocols choice for toxicity assessment. However, some questions remain to be answered, namely on the role of cell culture media components on the agglomeration state and mitigation of TiO2 NPs toxic effects.
Rapid Room-Temperature Synthesis of Mesoporous TiO2 Sub-Microspheres and Their Enhanced Light Harvesting in Dye-Sensitized Solar Cells
Authors: Alduraibi Mohammad , Hezam Mahmoud , Al-Ruhaimi Bader , El-Toni Ahmed Mohamed , Algarni Ahmad , Abdel-Rahman M. , Qing Wang , Aldwayyan Abdullah
Published: February 2020

Submicron sized mesoporous spheres of TiO2 have been a potential alternative to overcome the light scattering limitations of TiO2 nanoparticles in dye-sensitized solar cells (DSSCs). Currently available methods for the growth of mesoporous TiO2 sub-microspheres involve long and relatively high temperature multi-stage protocols. In this work, TiO2 mesoporous sub-microspheres composed of ~5 nm anatase nanocrystallites were successfully synthesized using a rapid one-pot room-temperature CTAB-based solvothermal synthesis. X-Ray Diffraction (XRD) showed that the grown structures have pure anatase phase. Transmission electron microscopy (TEM) revealed that by reducing the surfactant/precursor concentration ratio, the morphology could be tuned from monodispersed nanoparticles into sub-micron sized mesoporous beads with controllable sizes (50–200 nm) and with good monodispersity as well. The growth mechanism is explained in terms of the competition between homogeneous nucleation/growth events versus surface energy induced agglomeration in a non-micelle CTAB-based soft templating environment. Further, dye-sensitized solar cells (DSSCs) were fabricated using the synthesized samples and characterized for their current-voltage characteristics. Interestingly, the DSSC prepared with 200 nm TiO2 sub-microspheres, with reduced surface area, has shown close efficiency (5.65%) to that of DSSC based on monodispersed 20 nm nanoparticles (5.79%). The results show that light scattering caused by the agglomerated sub-micron spheres could compensate for the larger surface areas provided by monodispersed nanoparticles.
Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes
Authors: Svadlakova Tereza , Hubatka Frantisek , Turanek Knotigova Pavlina , Kulich Pavel , Masek Josef , Kotoucek Jan , Macak Jan , Motola Martin , Kalbac Martin , Kolackova Martina , Vankova Radka , Vicherkova Petra , Malkova Andrea , Simeckova Pavlina , Volkov Yuri , Prina-Mello Adriele , Kratochvilova Irena , Fiala Zdenek , Raska Milan , Krejsek Jan , Turanek Jaroslav
Published: February 2020

Carbon-based nanomaterials (C-BNM) have recently attracted an increased attention as the materials with potential applications in industry and medicine. Bioresistance and proinflammatory potential of C-BNM is the main obstacle for their medicinal application which was documented in vivo and in vitro. However, there are still limited data especially on graphene derivatives such as graphene platelets (GP). In this work, we compared multi-walled carbon nanotubes (MWCNT) and two different types of pristine GP in their potential to activate inflammasome NLRP3 (The nod-like receptor family pyrin domain containing 3) in vitro. Our study is focused on exposure of THP-1/THP1-null cells and peripheral blood monocytes to C-BNM as representative models of canonical and alternative pathways, respectively. Although all nanomaterials were extensively accumulated in the cytoplasm, increasing doses of all C-BNM did not lead to cell death. We observed direct activation of NLRP3 via destabilization of lysosomes and release of cathepsin B into cytoplasm only in the case of MWCNTs. Direct activation of NLRP3 by both GP was statistically insignificant but could be induced by synergic action with muramyl dipeptide (MDP), as a representative molecule of the family of pathogen-associated molecular patterns (PAMPs). This study demonstrates a possible proinflammatory potential of GP and MWCNT acting through NLRP3 activation.
Effective Inhibition of Candidiasis Using an Eco-Friendly Leaf Extract of Calotropis-gigantean-Mediated Silver Nanoparticles
Authors: Ali Enas M. , Abdallah Basem M.
Published: February 2020

The approaches used for the green biosynthesis of nanoparticles with clinical applications have been widely used in nanotechnology due to their potential to provide safe, eco-friendly, cost effective, high-stability, and high-loading-capacity nanoparticles. This study aimed to evaluate the anti-candidal activity of silver nanoparticles (AgNPs) biosynthesized using the aqueous leaf extract of Calotropis gigantea (CG) alone or in a combination with the plant extract of CG (AgNPs/CG). AgNPs were characterized using UV-Vis spectrophotometry, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The results of the standard disk diffusion method revealed that AgNPs alone displayed anti-candidal activity (11.33-mm inhibition zone), while AgNPs/CG displayed a strong synergistic anti-candidal activity (17.76-mm inhibition zone). Similarly, AgNPs/CG completely inhibited the growth of C. albicans after 4 h of incubation, as measured using the time-kill assay. In addition, AgNPs/CG inhibited the dimorphic transition of C. albicans and suppressed both the adhesion and the biofilm formation of C. albicans by 41% and 38%, respectively. The treatment of Candida. albicans with AgNPs/CG showed a significant inhibition of the production of several antioxidant enzymes. Interestingly, AgNPs/CG did not show any cytotoxicity in animal cells, including the MCF-7 cell line and primary mouse bone marrow-derived mesenchymal stem cells (mBMSCs), at the concentration used to completely inhibit the dimorphic transition of C. albicans. In conclusion, we identified AgNPs/CG as a promising natural-product-based nanoparticle that can potentially be used as an anti-candidal drug.
MIL-88A Metal-Organic Framework as a Stable Sulfur-Host Cathode for Long-Cycle Li-S Batteries
Authors: Benítez Almudena , Amaro-Gahete Juan , Esquivel Dolores , Romero-Salguero Francisco José , Morales Julián , Caballero Álvaro
Published: February 2020

Lithium-sulfur (Li-S) batteries have received enormous interest as a promising energy storage system to compete against limited, non-renewable, energy sources due to their high energy density, sustainability, and low cost. Among the main challenges of this technology, researchers are concentrating on reducing the well-known “shuttle effect” that generates the loss and corrosion of the active material during cycling. To tackle this issue, metal-organic frameworks (MOF) are considered excellent sulfur host materials to be part of the cathode in Li-S batteries, showing efficient confinement of undesirable polysulfides. In this study, MIL-88A, based on iron fumarate, was synthesised by a simple and fast ultrasonic-assisted probe method. Techniques such as X-ray diffraction (XRD), Raman spectroscopy, Thermogravimetric Analysis (TGA), Scanning Electron Microscopy (SEM), and N2 adsorption/desorption isotherms were used to characterise structural, morphological, and textural properties. The synthesis process led to MIL-88A particles with a central prismatic portion and pyramidal terminal portions, which exhibited a dual micro-mesoporous MOF system. The composite MIL-88A@S was prepared, by a typical melt-diffusion method at 155 °C, as a cathodic material for Li-S cells. MIL-88A@S electrodes were tested under several rates, exhibiting stable specific capacity values above 400 mAh g−1 at 0.1 C (1C = 1675 mA g−1). This polyhedral and porous MIL-88A was found to be an effective cathode material for long cycling in Li-S cells, retaining a reversible capacity above 300 mAh g−1 at 0.5 C for more than 1000 cycles, and exhibiting excellent coulombic efficiency.
Walnut Polyphenol Extract Protects against Malathion- and Chlorpyrifos-Induced Immunotoxicity by Modulating TLRx-NOX-ROS
Authors: Zhao Yue , Fan Chang , Zhang Ao , Zhang Yue , Wang Fengjun , Weng Qiang , Xu Meiyu
Published: February 2020

Malathion (MT) and chlorpyrifos (CPF) are immunotoxic organophosphate pesticides that are used extensively in agriculture worldwide. Dietary polyphenols protect against a variety of toxins. In this study, walnut polyphenol extract (WPE) prevents MT- or CPF-induced toxicity to splenic lymphocytes in vitro. WPE promotes the proliferation of MT-exposed splenocytes, as indicated by increases in the proportions of splenic T-lymphocyte subpopulations (CD3+, CD4+, and CD8+ T cells) and levels of T-cell-related cytokines interleukin (IL)-2, interferon-γ, IL-4, and granzyme B, and decreases the apoptosis-associated proteins Bax and p53. WPE also significantly enhances the proliferation of CPF-exposed splenic B lymphocytes (CD19+ B cells) and levels of the B-cell-related cytokine IL-6, leading to decreases of the apoptosis-associated proteins Bax and p53. These effects are related to reduced production of reactive oxygen species (ROS), as evidenced by normalized hydroxyl radical (•OH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and glutathione (GSH) levels, which are associated with decreased expression of NADPH oxidase 2 (NOX2) and dual oxidase 1 (DUOX1). WPE inhibits the production of ROS and expression of NOX by regulating toll-like receptors 4 and 7 in MT- and CPF-exposed splenic lymphocytes. In conclusion, WPE protects against MT- or CPF-mediated immunotoxicity and inhibits oxidative damage by modulating toll-like receptor (TLR)x-NOX-ROS.
Relative Validity and Reproducibility of a Short Food Frequency Questionnaire to Assess Nutrient Intakes of New Zealand Adults
Authors: Sam Cecilia Ho Yan , Skidmore Paula , Skeaff Sheila , Parackal Sherly , Wall Clare , Bradbury Kathryn E.
Published: February 2020

There is no recent validated short food frequency questionnaire (FFQ) for use in New Zealand (NZ) adults. This study aimed to evaluate the relative validity and reproducibility of a short FFQ in free-living NZ adults aged 30–59 years. A 57-item, semi-quantitative FFQ was developed and pre-tested. During a 12-month study period the FFQ was administrated twice with a 9-month interval between administrations. Four two-day diet records were collected at months 0, 3, 6, and 9 and a blood sample was taken at month 9. Spearman correlations were used to evaluate the validity of the FFQ with the eight-day diet records and selected biomarkers. Cross-classification analysis and the Bland–Altman method were used to assess the agreement between the FFQ and the diet record. Reproducibility over nine months was assessed using intra-class correlations. A total of 132 males and females completed both FFQs, the eight-day diet record, and provided a blood sample. The highest energy-adjusted correlation coefficients were observed for alcohol (0.81), cholesterol (0.61), and carbohydrate (0.61), with the lowest for sodium (0.29), thiamin (0.33), and niacin equivalents (0.34). More than three quarters of the participants were correctly classified into the same or adjacent quartile for most nutrients, with a low proportion of participants being grossly misclassified (<10%). For most nutrients, the limits of agreement from the Bland–Altman analyses were between 50% and 250%. A positive correlation was observed between dietary intakes and plasma biomarkers for all selected nutrients. The FFQ showed moderate to good reproducibility, with almost all reliability coefficients ranging from 0.60 to 0.80. This short FFQ was shown to validly and reliably rank individuals by their habitual intake of most major nutrients, indicating that the FFQ will offer a time-efficient way to assess the nutrient intake of NZ adults in future research.
Brain Alterations in High Fat Diet Induced Obesity: Effects of Tart Cherry Seeds and Juice
Authors: Micioni Di Bonaventura Maria , Martinelli Ilenia , Moruzzi Michele , Micioni Di Bonaventura Emanuela , Giusepponi Maria , Polidori Carlo , Lupidi Giulio , Tayebati Seyed , Amenta Francesco , Cifani Carlo , Tomassoni Daniele
Published: February 2020

Evidence suggests that obesity adversely affects brain function. High body mass index, hypertension, dyslipidemia, insulin resistance, and diabetes are risk factors for increasing cognitive decline. Tart cherries (Prunus Cerasus L.) are rich in anthocyanins and components that modify lipid metabolism. This study evaluated the effects of tart cherries on the brain in diet-induced obese (DIO) rats. DIO rats were fed with a high-fat diet alone or in association with a tart cherry seeds powder (DS) and juice (DJS). DIO rats were compared to rats fed with a standard diet (CHOW). Food intake, body weight, fasting glycemia, insulin, cholesterol, and triglycerides were measured. Immunochemical and immunohistochemical techniques were performed. Results showed that body weight did not differ among the groups. Blood pressure and glycemia were decreased in both DS and DJS groups when compared to DIO rats. Immunochemical and immunohistochemical techniques demonstrated that in supplemented DIO rats, the glial fibrillary acid protein expression and microglial activation were reduced in both the hippocampus and in the frontal cortex, while the neurofilament was increased. Tart cherry intake modified aquaporin 4 and endothelial inflammatory markers. These findings indicate the potential role of this nutritional supplement in preventing obesity-related risk factors, especially neuroinflammation.
Interplay between Dietary Polyphenols and Oral and Gut Microbiota in the Development of Colorectal Cancer
Authors: Cueva Carolina , Silva Mariana , Pinillos Iris , Bartolomé Begoña , Moreno-Arribas M. Victoria
Published: February 2020

Colorectal cancer (CRC) is the third most diagnosed type of cancer worldwide. Dietary features play an important role in its development, and the involvement of human microbial communities in this pathology has also recently been recognized. Individuals with CRC display alterations in gut bacterial composition and a notably higher abundance of putative oral bacteria in colonic tumors. Many experimental studies and preclinical evidence propose that dietary polyphenols have a relevant role in CRC development and progression, mainly attributed to their immunomodulatory activities. Furthermore, polyphenols can modulate oral and gut microbiota, and in turn, intestinal microbes catabolize polyphenols to release metabolites that are often more active and better absorbed than the original phenolic compounds. The current study aimed to review and summarize current knowledge on the role of microbiota and the interactions between dietary polyphenols and microbiota in relation to CRC development. We have highlighted the mechanisms by which dietary polyphenols and/or their microbial metabolites exert their action on the pathogenesis and prevention of CRC as modulators of the composition and/or activity of oral and intestinal microbiota, including novel screening biomarkers and possible nutritional therapeutic implications.