bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal calorie restriction threshold: effect of FATmax exercise combined with different proportions of calorie restriction on hypercholesterolemia
Authors: Wu Yuetong , Zhang Li , Li Jing , Xue Boyang , Shang Wenyuan , Lu Yingli
Published: January 2025

Background/Objectives

To evaluate the impact of maximal fat oxidation intensity exercise combined with calorie restriction intervention on lipid-related parameters in a hypercholesterolemic population, and to determine if an optimal range of calorie restriction exists for effectively enhancing blood lipid profiles.

Methods

A 4-week intervention study combined exercise and calorie restriction for 64 patients aged 18–60 with secondary hypercholesterolemia. Ultimately, 43 participants completed the study. The dietary intervention adhered to the principles of a balanced diet, with meal plans designed to provide three meals per day for the duration of the study. Each subject’s daily calorie intake was set to match their individual resting energy expenditure (REE) plus varying proportions of physical activity (PA) calories. Participants were divided into four groups based on these proportions: REE only, REE + PA33%, REE + PA67%, and REE + PA100%. FATmax exercises were conducted 5 times per week, lasting 1 h each.

Results

1) Compared with baseline, subjects’ body weight, fat mass and body fat rate decreased significantly; fat-free mass also decreased significantly in the REE, REE + PA33%, and REE + PA67% groups. 2) Subjects’ serum TC decreased significantly; serum LDL-C and ApoB decreased significantly in the REE, REE + PA33%, and REE + PA67% groups; there were no significant changes in serum HDL-C and ApoA1. 3) Serum PCSK9 was significantly decreased in the REE and the REE + PA 67% groups; serum LDLR was significantly decreased in all groups of subjects. 4) Between the groups, the rate of change in serum LDL-C was significantly different.

Conclusion

FATmax exercise combined with proper proportions of calorie restriction can significantly decrease serum cholesterol levels and fat mass in hypercholesterolemic patients. Nevertheless, it is misleading to assume that a drastic reduction in calorie intake invariably results in superior outcomes. Optimal cost-effectiveness may be achieved within a calorie restriction range of REE + PA33-67%.
Integrative analyses of morpho-physiological, biochemical, and transcriptomic reveal the seedling growth response of Pinus yunnanensis to nitrogen and phosphorus fertilization
Authors: Xu Junfei , Li Xiyan , Chen Shi , Chen Lin , Tang Junrong , Chen Peizhen , Cai Nianhui , Xu Yulan
Published: January 2025

Appropriate nitrogen (N) and phosphorus (P) fertilization is critical for plant growth and production. Pinus yunnanensis, a silvicultural tree in southwestern China, faces economic and ecological limitations due to nutrient deficiency in the soils in its distribution areas. The slow growth of this species during the seedling stage exacerbates these problems. Therefore, it is important to realize the regulating effects of N and P proportioning fertilization on seedling growth to enhance nutrient-use efficiency. In this study, variations in morphological, physiological, and biochemical characteristics of seedlings were analyzed under nine treatments of NP proportioning in an open nursery using a regression design. Growth in height and basal diameter increased and showed an approximate tendency in all treatments. The maximum biomass accumulation was observed at 480 d after treatment in roots of T5 (14.714 g) (application N 0.4 g·per−1 and P 3 g·per−1), stems of T5 (12.654 g), leaves of T9 (24.261 g) (application N 0.8 g·per−1 and P 6 g·per−1), aboveground parts of T9 (35.402 g) and individuals of T5 (49 g). The total chlorophyll content peaked in the leaves at 120 d and was correlated with the peak levels of N, P, and K in leaves. The content and reserves of nutrient elements in the organs of seedlings subjected to NP proportioning were significantly higher than those in unfertilized seedlings. Analysis of nutrient utilization efficiency revealed that T5 demonstrated superior seedling growth performance. Appropriate fertilization dosage of N and P for P. yunnanensis seedlings in this study was 0.32 g·per−1–0.58 g·per−1 and 3.02 g·per−1–4.95 g·per−1 respectively, using path analysis and regression equation. Transcriptomic sequencing revealed that there were 2,301 DEGs between T5 and T1 (control), which were involved in the uptake and assimilation of nutrients, biosynthesis of phytohormones and secondary metabolites, and photosynthesis. Additionally, the abundance of genes involved in cell division and proliferation, cellulose biosynthesis, and cell wall extension were dramatically upregulated, which potentially correlated with enhanced seedling growth. In conclusion, this study provides comprehensive information on the response of seedlings to varying proportions of N and P and may promote the growth of P. yunnanensis seedlings by optimizing the proportion of N and P in fertilizers.
Dual stress, equivalent harm? hypothesizing on the type of interactions between waterlogging and high temperature
Authors: Ploschuk Rocío A. , Savin Roxana , Slafer Gustavo A.
Published: January 2025

Episodes of extreme weather, such as high temperatures and heavy rains causing waterlogging, have been becoming more frequent due to climate change, posing risks to crops and reducing growth and yield. While the impact of these stresses has been individually studied, there is a significant gap in understanding their combined effects within the same growing season. There were only 15 studies in the rigorous literature addressing the combined impact of high temperatures and waterlogging. None explicitly examined whether these combined effects were additive (penalties close to the sum of the individual penalties), synergistic (more severe penalties), or antagonistic (less severe penalties). We aimed to propose a sound hypothesis on the most likely type of interaction between these two stressors. Reviewing the scarce literature we found, against expectations, that antagonistic interactions were most common, followed by cases of additive effects, with synergistic interactions being rare. Notably, while the primary concern of virtually all studies was the impact on crop yield, most of them focused exclusively on leaf-level traits, whose responses did not correlate well with yield responses. This preliminary analysis provides solid roots for hypothesizing that waterlogging and high temperatures interact antagonistically; i.e., that plants might develop some resilience when exposed to one stress, potentially reducing the impact of the other. Should this hypothesis be accepted, considering not only physiological traits but also, and mainly, yield in major crops, there would be a less pessimistic view on the expected outcome of the increased frequency of crops being exposed to combined high temperature and waterlogging.
Inhibition of histone deacetylase in Arabidopsis root calli promotes de novo shoot organogenesis
Authors: Pan Qinwei , Huang Ruirui , Xiao Qiong , Wu Xuting , Jian Baoxia , Xiang Yanan , Gan Lijun , Liu Zongrang , Li Yi , Gu Tingting , Liu Huawei
Published: January 2025

De novo organogenesis from somatic cells to the entire plant represents a remarkable biological phenomenon, but the underlying regulatory mechanism, particularly at the epigenetic level, remains obscure. In this work, we demonstrate the important role of histone deacetylases (HDACs) in shoot organogenesis. HDAC inhibition by trichostatin A (an HDAC inhibitor) at the callus induction stage promotes shoot formation in wounded roots and circumvents tissue wounding to initiate shoot regeneration in unwounded roots. This HDAC inhibition-mediated promotion of shoot organogenesis in wounded roots is associated with the concomitant upregulation of the wound signaling pathway (WOUND INDUCED DEDIFFERENTIATION 4, ENHANCER OF SHOOT REGENERATION1, ISOPENTENYLTRANSFERASE 5, CUP-SHAPED COTYLEDON 2 etc.) and the ARF-LBD pathway (AUXIN RESPONSE FACTOR 19, LATERAL ORGAN BOUNDARIES-DOMAIN 29, etc.) and the downregulation of auxin biosynthesis and reduced auxin content. Furthermore, inhibiting HDACs enhances the local enrichment of histone 3 lysine 9/lysine 14 acetylation at ISOPENTENYLTRANSFERASE 5, supporting the role of histone acetylation in its transcriptional regulation. On the other hand, the HDAC inhibition-associated activation of shoot organogenesis from unwounded roots is coupled with increased expression of the ARF-LBD pathway gene LATERAL ORGAN BOUNDARIES-DOMAIN 29 while bypassing the wound signaling or auxin biosynthetic genes. These findings provide novel insights into the regulatory mechanisms underlying de novo shoot organogenesis and lay a foundation for the improvement of plant transformation technologies.
Design and testing of a posture-adjusting precision metering device for high-speed maize planting
Authors: Dong Jianxin , Gao Xiaojun , Zheng Zhouzhou , Zhao Pengfei , Bi Yubin , Huang Yuxiang
Published: January 2025

Introduction

This study introduces a new technical method that involves adjusting the seed-filling posture and fluidizing the seed group to improve the capacity of mechanical seed-metering devices for high-speed maize planting. The design of a novel posture-adjusting seed-metering device is presented and its operating principle is described. In the seed-filling area, the seeds maintain the same postural characteristics and are detached from the seed group. This method creates favorable conditions for mechanical seed-metering devices to achieve effective seed-filling under high-speed planting conditions.

Methods

Firstly, the key parameters of the core component were analyzed. Secondly, the influences of the seed posture adjusting efficiency and seed flow distribution characteristics on the seed-filling effect were clarified by the EDEM simulation. Finally, the high-speed metering performance was optimized and validated using bench testing.

Results

The experiment demonstrated that the type A groove had the highest seed posture adjusting efficiency, a more uniform seed flow distribution, and a superior seed-filling effect as compared to the other groove types. The best metering performance was achieved with an opening angle of 21.6° and an opening width of 8.4 mm; this resulted in pass, repeat, and leak rates of 94.8%, 1.3%, and 3.9%, respectively. Within the speed range of 8–14 km/h, the posture-adjusting seed-metering device demonstrated a pass rate of over 94%, a repeat rate below 2.1%, and a leak rate below 3.9%.

Discussion

The designed device had a better high-speed planting capacity than a seed-metering device without a posture-adjusting mechanism, thus proving the effectiveness of the novel seed-filling method. These findings provide a reference for improving the high-speed planting capacity of mechanical seed-metering devices.
Plastid genome comparison and phylogenetic analyses of the Chinese group of medicinal species and related taxa within Asparagus genus
Authors: Xie Pingxuan , Wang Tinglu , Tan Jiawei , Fan Linyuan , Liu Changkun , Yan Hanjing
Published: January 2025

Background

Asparagus L. is a large genus widely distributed across the continents of the Old World. Among its members, approximately 14 species found in China are recognized as popular herbal medicines. However, accurate authentication of these medicinal species and their phylogenetic relationships with related taxa remains unresolved.

Methods

To identify simple sequence repeats (SSRs) and divergence hotspot regions appropriate for future authentication studies, as well as to infer the phylogenetic relationships among Asparagus species, we employed a plastid genome (plastome) dataset consisting of 25 Asparagus species (21 newly sequenced and four retrieved from GenBank), encompassing 12 Chinese medicinal species, for comparative and phylogenetic analyses.

Results

All Asparagus plastomes displayed a typical quadripartite structure with sizes ranging from 155,948 bp to 157,128 bp and harbored 114 unique genes (80 protein-coding genes, 30 tRNA genes, and four rRNA genes). IRscope and Mauve analyses indicated minimal structural variation among Asparagus plastomes. We detected between 79 to 95 SSRs across the plastomes; most were located in the large single-copy (LSC) region and primarily consisted of mono-nucleotide repeat sequences (especially A and T repeats). The genus displayed mono-, di-, tri-, tetra-, penta-, and hexa-nucleotide repeats, but with variations in types and numbers among different species. Additionally, we identified 12 special SSR motifs and seven divergent hotspot regions that may serve as potential molecular markers for future identification efforts. Phylogenetic analyses yielded a robust phylogeny for Asparagus taxa, which were split into Clades I, II, and III. Notably, medicinal Asparagus species were mainly found in Clade III. Although the phylogenetic relationships of most Asparagus species aligned with previous study findings, the phylogenetic positions of A. munitus, A. subscandens, A. gobicus, and A. dauricus were newly determined.

Conclusions

The plastomes of Asparagus are largely conserved in terms of genome structure, size, gene content, and arrangement. Nevertheless, SSRs analyses revealed significant interspecific polymorphism within Asparagus. In addition, special SSR motifs and divergent hotspot regions identified from Asparagus plastomes provided reference for subsequent identification investigations. The plastome-based phylogeny provided preliminary insights into the relationships among the Chinese group of medicinal species and related taxa within Asparagus. Overall, this study offers a wealth of informative genetic resources pertinent to Asparagus, thereby enhancing our understanding of its evolution and laying a foundation for species identification, assessment of genetic population diversity, as well as the exploration and conservation of germplasm resources.
The transcriptional analysis of pepper shed light on a proviral role of light-harvesting chlorophyll a/b binding protein 13 during infection of pepper mild mottle virus
Authors: Lin Weihong , Zhang Shugen , Zhang Hao , Deng Xiaomei , Jiang Tong , Chen Xifeng , Dong Laihua , Yan Qin , Zang Lianyi , Xing Yongping , Wang Zhenquan , Zhang Qin , Du Kaitong , Shen Huolin , Zhang Junmin , Zhou Tao
Published: January 2025

Pepper mild mottle virus (PMMoV), a member of the genus Tobamovirus, causes severe damage on pepper worldwide. Despite its impact, the pathogenicity mechanisms of PMMoV and the pepper plant’s response to infection remain poorly understood. Here, we compared the transcriptomic changes in a susceptible pepper inbred line 21C241 with a resistant inbred line 21C385 seedlings, following systemic PMMoV infection using RNA sequencing. Our results revealed that PMMoV induced more pronounced mosaic symptoms and higher viral accumulation levels in the susceptible line 21C241 compared to the resistant line 21C385. We identified 462 and 401 differentially expressed genes (DEGs) in the systemically-infected leaves of the susceptible and resistant lines, respectively, when compared to their healthy counterparts. The majority of these DEGs were involved in photosynthesis and the biosynthesis of secondary metabolites, with 28 DEGs exhibiting distinct expression patterns between the two lines. Notably, the expression level of the chlorophyll a-b binding protein 13 (CAB13) was significantly up-regulated in resistant line 21C385 following PMMoV infection. Functional analysis through silencing of CAB13 in pepper and Nicotiana benthamiana demonstrated a reduction in PMMoV accumulation, suggesting that CAB13 plays a positive role in facilitating PMMoV infection in pepper plants. Taken together, our findings highlight the distinct gene expression profiles between susceptible and resistant pepper lines in response to PMMoV infection and confirm the proviral role of CAB13. This study provides valuable insights into the molecular mechanisms underlying resistance and susceptibility in pepper plants and may inform future strategies for disease management.
Kindergarten teachers’ emotional intelligence and surface acting: the chain mediating effects of self-efficacy and work engagement
Authors: Sun Shucun , Yan Zhonglian , Sun Caixia
Published: January 2025

Background

Emotional labor, distinct from physical and mental labor, has gained significant attention in contemporary organizational psychology research. As a strategy for performing emotional labor, surface acting refers to the process of faking emotions to meet the demands of organizations’ norms. This study aimed to investigate the relationship between emotional intelligence and surface acting among Chinese kindergarten teachers, focusing on the mediating role of self-efficacy and work engagement.

Methods

Integrating the conservation of resources theory and the job demands-resources model, a questionnaire survey was conducted among 1,017 Chinese kindergarten teachers using Wong & Law Emotional Intelligence Scale, Self-Efficacy Scale, Utrecht Work Engagement Scale, and Surface Acting Scale. SPSS 26.0 was used to conduct descriptive statistics and correlation tests. Structural equation modeling and mediation analysis were constructed using AMOS 28.0.

Results

The results showed reveals several key findings. Firstly, kindergarten teachers’ emotional intelligence significantly positively predicted surface acting rather than negatively. Secondly, self-efficacy and work engagement, respectively, mediate the relationship between emotional intelligence and surface acting in kindergarten teachers. Additionally, self-efficacy and work engagement have chain mediating effects in the relationship between kindergarten teachers’ emotional intelligence and surface acting.

Conclusion

The research findings reveal the influencing mechanism of kindergarten teachers’ emotional intelligence on surface acting, providing a theoretical basis and practical implications for understanding and promoting the reasonable use of surface acting by kindergarten teachers in the Chinese context.
Understanding perspective-taking in multiparty conversations: insights from Mandarin nouns
Authors: Zheng Xiaobei , Sun Chao
Published: January 2025

Individuals frequently adopt others’ perspectives both when interpreting language and when formulating their own responses in conversation. This experiment tested how participants used perspective information to resolve references for bare nouns in Mandarin. Specifically, it explored whether, when faced with two interlocutors, participants distinguished between each individual’s perspective or considered both as a whole. Using a classical referential game, the study manipulated the visual perspectives of two partners. In Experiment 1, both speakers had the same seating direction and visual field, and the results showed that participants equally took their perspectives into account above chance levels, providing a baseline finding for referential resolution of Mandarin bare nouns in perspective-taking studies. In Experiment 2, both speakers had the same seating direction but one of them shared the larger portion of visual field with the participants. The results showed that participants took the perspectives of the two speakers independently, while also comparing the perspectives of both interlocutors to facilitate quicker and more accurate referential resolution. These findings demonstrate that perspective-taking is a complex and dynamic process, providing evidence for the study of perspective-taking in Mandarin and contributing insights into comprehension processing in multiparty conversations.
Bridging the gap: aligning physical work capacity testing with actual endurance performance in military settings
Authors: Drozd Jan , Neubauer Jiří , Sekanina Jiří , Sedlačík Marek
Published: January 2025

Physical fitness tests are essential for evaluating the endurance capabilities of military personnel. In many armed forces, including the Czech Republic’s, the Physical Working Capacity at a heart rate of 170 beats per minute (PWC 170) test is used to predict performance on the 12-min Cooper run, a widely accepted measure of endurance. However, concerns exist regarding the accuracy of the currently used conversion between PWC 170 test results, specifically W170/kg (watts per kilogram of body weight), and actual 12-min run performance. This study directly investigates the relationship between W170/kg and 12-min run times among military recruits and students (military personnel) from a university with a military program. Utilizing regression analysis, we found a significant positive correlation between W170/kg and 12-min run performance. However, the currently used conversion significantly underestimated actual 12-min run performance across all analyzed groups. These findings highlight a critical need to revise the existing conversion standard between W170/kg and 12-min run performance to ensure a more accurate and effective assessment of endurance capabilities in military personnel.
Personalizing AI tools for second language speaking: the role of gender and autistic traits
Authors: Du Yiran , Wang Chenghao , Zou Bin , Xia Yinan
Published: January 2025

Introduction

It is important to consider individual differences in research on educational technology. This study investigates the interplay between autistic traits, gender, and the perception of artificial intelligence (AI) tools designed for second language (L2) speaking practice, contributing to a deeper understanding of inclusive educational technology.

Methods

A sample of 111 university students completed the Broad Autism Phenotype Questionnaire (BAPQ) to measure autistic traits (AU) and their sub-traits Aloof (AF), Rigid (RD), and Pragmatic Language (PL). Perceptions of AI tools were assessed across five dimensions: Perceived Usefulness (PU), Perceived Ease of Use (PEOU), Attitude (AT), Behavioral Intention (BI), and Usage Behavior (UB). The study utilized correlation and regression analyses to examine relationships between these variables, while exploring gender-specific moderating effects.

Results

Key findings revealed no significant gender differences in autistic traits or overall perceptions of AI tools. Contrary to expectations, autistic traits were negatively correlated with perceptions of AI tools, suggesting that current AI designs may not adequately support individuals with pronounced autistic traits. Additionally, gender moderated some relationships, with males displaying stronger associations between autistic traits and both PEOU and UB.

Discussion

This research bridges critical gaps by linking neurodiversity and gender to technology acceptance, advancing the field’s understanding of individual differences in AI-based language learning. It underscores the importance of designing personalized and adaptive educational tools that address diverse learner needs, promoting inclusivity and effectiveness in L2 practice.
Case report: Non-linear evolution of oxytocin informs YBOCS changes post-DBS of the bed nucleus of the stria terminalis for treatment resistant OCD
Authors: Belge Jean-Baptiste , Geenen Vincent , Salado Anne L. , Kaschten Bruno , Martin Didier , Scantamburlo Gabrielle
Published: January 2025

Introduction

Obsessive-compulsive disorder (OCD) is a challenging neuropsychiatric condition with a subset of patients remaining refractory to conventional treatments. Deep brain stimulation (DBS) of the bed nucleus of the stria terminalis (BNST) has shown promise for severe, treatment-resistant OCD. This case report examines the relationship between plasma oxytocin levels and OCD symptom severity following BNST-DBS.

Methods

A 36-year-old patient with long-standing, treatment-resistant OCD underwent stereotactic implantation of DBS electrodes at the BNST. Postoperative assessments included OCD symptom severity using the Yale-Brown Obsessive Compulsive Scale (YBOCS) and plasma oxytocin levels, measured at 12 time points over three years. Longitudinal and correlational analyses were performed using linear and polynomial regression models.

Results

Non-linear trends in oxytocin levels were identified, with polynomial regression revealing a significant quadratic term, suggesting a parabolic trend. Strong positive correlations were found between changes in oxytocin levels and YBOCS total, obsession, and compulsion scores.

Conclusion

The findings suggest a significant non-linear evolution of oxytocin levels and a positive correlation with OCD symptom changes following BNST-DBS. Oxytocin levels could serve as a biomarker for DBS efficacy if this finding is replicated in larger studies.
Spirituality and mental health – investigating the association between spiritual attitudes and psychosomatic treatment outcomes
Authors: Hinterberger Thilo , Walter Nike
Published: January 2025

Background

The relationship between spirituality and mental health has garnered attention, fostering overall well-being. Spirituality, posited as a protective factor, may enhance resilience and provide meaning, thus benefiting mental health. This study aims to identify spirituality-associated factors influencing clinical outcomes in psychosomatic inpatients and validate the Transpersonal Spirituality Inventory (TSI).

Methods

The study involved 4952 psychosomatic inpatients completing the Transpersonales Vertrauen (TPV) and 7739 patients completing the TSI, with assessments conducted at admission and discharge. Additional instruments included the ISR for symptom rating and the LK-18 for life skills. Factor analysis and Spearman’s rank correlation were used to evaluate the validity of TSI and the relationship between spirituality and clinical outcomes.

Results

Factor analysis confirmed the TSI’s two-factor structure: “centered connectedness” (F1) and “transcendent orientation” (F2), with satisfactory internal consistency (Cronbach’s α = 0.824 for F1 and 0.644 for F2). Higher spirituality levels, particularly in transpersonal trust and centered connectedness, correlated with lower depression and psychosomatic symptoms (ISR). Although these correlations were generally weak, significant associations were found between spirituality and life competences, particularly in meaning, belief, and values (r = .595 for TPV and .598 for TSI F1).

Conclusion

Despite correlations between psychosomatic health and spirituality as measured with TPV and TSI were rather small several spirituality items could be identified which seem to have a prominent connection to the diagnosis and development of psychosomatic health. Specific spiritual attitudes, such as access to inner stillness and connection to a greater whole, were linked to positive treatment responses and reduced symptom burden. These findings suggest potential benefits in incorporating spirituality into psychosomatic treatments, though its complex and multifaceted nature warrants further investigation.
Individual differences in training time in the rat gambling task are unrelated to subsequent decision-making strategies
Authors: Lindberg Frida A. , Kagios Christakis , Tjernström Nikita , Roman Erika
Published: January 2025

Introduction

Decision-making requires individuals to perceive probabilities and risks associated with different options. The Iowa gambling task (IGT) is a widely used instrument that assesses decision-making under uncertainty and risk by varying monetary reinforcer/loss contingencies. The rat gambling task (rGT), based on the IGT, is a preclinical test using varying number of palatable reinforcers as wins and different duration of timeouts as punishment, mimicking losses. The rGT requires extensive operant training prior to the free choice sessions. The aim of the present study was to investigate if task acquisition and number of training days affected subsequent individual differences in decision-making strategies in the rGT, and if behavioral profiles impacted on task learning.

Method

Training time and performance of 70 male Lister Hooded rats from previously published studies were herein used to investigate whether learning time affected later decision-making strategies in the free choice rGT. Behavioral profiles generated from a subset of animals were used to study the impact of underlying behavior on learning time.

Results

There were differences in training days between fast, intermediate and slow learners. However, time required to acquire the rGT did not affect subsequent decision-making strategies in the free choice rGT. Finally, learning time was independent of underlying behavioral profiles.

Discussion

In conclusion, neither decision-making strategies in the rGT nor behavioral profiles were correlated or differed between animals with different learning speed. This suggests that the large variation in training time between animals is unrelated to subsequent decision-making strategies during free choice rGT. Such information is valuable for researchers using the rGT.
Maximising translational value of the Iowa gambling task in preclinical studies through the use of the rodent touchscreen
Authors: Pratt Judith A. , Morris Brian J.
Published: January 2025

The Iowa gambling task is widely employed to assess the evaluation of risk versus reward contingencies, and how the evaluations are implemented to gain advantageous returns. The cognitive processes involved can be compromised in psychiatric conditions, leading to the development of analogous tasks with translational value for use in rodents. The rodent touchscreen apparatus maximises the degree of similarity with the human task, and in this review we provide an outline of the use of rodent touchscreen gambling tasks in preclinical studies of psychiatric conditions. In particular, we describe how the basic task has been adapted to probe the relative contributions of different neurotransmitter systems, and specific aspects of cognition. We then offer a perspective on how the task might be employed most beneficially in future studies.
Mobile phone addiction and negative emotions: an empirical study among adolescents in Jiangxi Province
Authors: Luo Jin , Cai Guanqing , Zu Xiaofang , Huang Qiming , Cao Qing
Published: January 2025

Background

The relationship between mobile phone addiction and negative affect among adolescents is characterized by intricate interconnections. These factors have an impact on the emotional and psychological well-being of young people. While previous studies have provided preliminary insights into this relationship, further in-depth exploration and research is required to fully understand the complex mechanisms behind these relationships and to identify any group differences.

Methods

This study collected questionnaire data from 493 adolescents aged 17-22 years old in Jiangxi Province. The data were analyzed using Pearson correlation analysis, restricted cubic spline (RCS), and structural equation modelling (SEM) with the aim of exploring the mechanisms and differences in the effects of mobile phone addiction on negative emotions of different youth groups.

Results

The findings of the study indicated notable discrepancies in mobile phone addiction by gender and place of residence. Secondly, mobile phone addiction among adolescents positively drives negative emotions. The findings revealed that negative emotions triggered by mobile phone addiction were significantly higher among rural adolescents than urban adolescents; male adolescents exhibited higher levels than female adolescents in the same place of residence. Additionally, family factors, physical exercise and psychological resilience can directly or indirectly inhibit mobile phone addiction among adolescents. Finally, adolescents’ psychological resilience can significantly alleviate the negative emotions associated with mobile phone addiction. Physical exercise and family factors can reduce the negative emotional impact of mobile phone addiction by increasing psychological resilience.

Conclusions

This study employs the relationship between mobile phone addiction and negative emotions in adolescents, reveals the complex mechanisms and group differences behind these relationships, and provides a fresh perspective for understanding the impact of mobile phone addiction on adolescents’ mental health, as well as a scientific basis for the development of effective interventions.
Low-dose ionizing radiation and the exposure–lag response: protocol for a prospective cohort study on The Health Effects of Chongqing Occupational Radiation Workers
Authors: Qin Xiao-Ling , Huang Qiang , Zhang Han-Wen , Zeng Yi , Lin Xian-Shu , Fan Xiao-Yuan , Diao Jun , Chen Cheng-Zhi , Cheng Shu-Qun , Yuan Fang , He Jun-Lin , Li Wei , Xia Yin-Yin
Published: January 2025

Introduction

Although the effects of ionizing radiation on radiation workers have been extensively studied in China, no prospective cohort study has been conducted in Chongqing. Furthermore, previous cohorts have not provided a broad-gauge assessment of the temporal relationship between low-dose occupational radiation exposure and the risk of health outcomes.

Methods

A prospective cohort study will be carried out focusing on radiation workers in Chongqing. Health examination outcomes and radiation dose monitoring data will be collected and analyzed using the distributed lag non-linear model (DLNM) combined with generalized additive model (GAM) or generalized linear model (GLM) to evaluate the exposure-lag response relationship.

Discussion

Our study will enhance our understanding of the exposure-lag response association between occupational radiation exposure and the health of radiation workers based on DLNM.

Clinical trial registration

Chinese Clinical Trials Registry, ChiCTR2400081804.
Bridging the gap: a practical step-by-step approach to warrant safe implementation of large language models in healthcare
Authors: Workum Jessica D. , van de Sande Davy , Gommers Diederik , van Genderen Michel E.
Published: January 2025

Large Language Models (LLMs) offer considerable potential to enhance various aspects of healthcare, from aiding with administrative tasks to clinical decision support. However, despite the growing use of LLMs in healthcare, a critical gap persists in clear, actionable guidelines available to healthcare organizations and providers to ensure their responsible and safe implementation. In this paper, we propose a practical step-by-step approach to bridge this gap and support healthcare organizations and providers in warranting the responsible and safe implementation of LLMs into healthcare. The recommendations in this manuscript include protecting patient privacy, adapting models to healthcare-specific needs, adjusting hyperparameters appropriately, ensuring proper medical prompt engineering, distinguishing between clinical decision support (CDS) and non-CDS applications, systematically evaluating LLM outputs using a structured approach, and implementing a solid model governance structure. We furthermore propose the ACUTE mnemonic; a structured approach for assessing LLM responses based on Accuracy, Consistency, semantically Unaltered outputs, Traceability, and Ethical considerations. Together, these recommendations aim to provide healthcare organizations and providers with a clear pathway for the responsible and safe implementation of LLMs into clinical practice.
Enhancing access to primary care is critical to the future of an equitable health service: using process visualisation to understand the impact of national policy in the UK
Authors: Litchfield Ian , Gale Nicola Kay , Greenfield Sheila , Shukla David , Burrows Micheal
Published: January 2025

Access to UK general practice is complicated by the need to provide equitable and universal care within a system adapting to workforce challenges, digital innovation, and unprecedented demand. Despite the importance of accessing primary care in meeting the overall aim of delivering equitable care, this is the first time the direct and indirect influence of policies intended to facilitate access have been systematically explored. Further consideration by policymakers is needed to accommodate the difference between what patients need and what patients want when accessing primary care, and the differences in their ability to utilise digital options. The designation of care was hindered by long-standing issues of reliable data and variations in the interpretation of local and national protocols and guidelines.
Vision-based manipulation of transparent plastic bags in industrial setups
Authors: Adetunji F. , Karukayil A. , Samant P. , Shabana S. , Varghese F. , Upadhyay U. , Yadav R. A. , Partridge A. , Pendleton E. , Plant R. , Petillot Y. R. , Koskinopoulou M.
Published: January 2025

Introduction

This paper addresses the challenges of vision-based manipulation for autonomous cutting and unpacking of transparent plastic bags in industrial setups, contributing to the Industry 4.0 paradigm. Industry 4.0, emphasizing data-driven processes, connectivity, and robotics, enhances accessibility and sustainability across the value chain. Integrating autonomous systems, including collaborative robots (cobots), into industrial workflows is crucial for improving efficiency and safety.

Methods

The proposed system employs advanced Machine Learning algorithms, particularly Convolutional Neural Networks (CNNs), for identifying transparent plastic bags under diverse lighting and background conditions. Tracking algorithms and depth-sensing technologies are integrated to enable 3D spatial awareness during pick-and-place operations. The system incorporates vacuum gripping technology with compliance control for optimal grasping and manipulation points, using a Franka Emika robot arm.

Results

The system successfully demonstrates its capability to automate the unpacking and cutting of transparent plastic bags for an 8-stack bulk-loader. Rigorous lab testing showed high accuracy in bag detection and manipulation under varying environmental conditions, as well as reliable performance in handling and processing tasks. The approach effectively addressed challenges related to transparency, plastic bag manipulation and industrial automation.

Discussion

The results indicate that the proposed solution is highly effective for industrial applications requiring precision and adaptability, aligning with the principles of Industry 4.0. By combining advanced vision algorithms, depth sensing, and compliance control, the system offers a robust method for automating challenging tasks. The integration of cobots into such workflows demonstrates significant potential for enhancing efficiency, safety, and sustainability in industrial settings.
Association of over-the-counter mouthwash use with markers of nitric oxide metabolism, inflammation, and endothelial function—a cross-sectional study
Authors: Guo Kai , Joshipura Kaumudi , Ricart Karina , Patel Rakesh P. , Gower Barbara A. , Andriankaja Oelisoa Mireille , Morou-Bermudez Evangelia
Published: January 2025

Introduction

Regular use of mouthwash can disrupt nitrate reduction by oral bacteria and may affect systemic nitric oxide (NO) levels, which are important for inflammation and endothelial function. We aim to assess the association between over-the-counter (OTC) mouthwash use and nitrate/nitrite, markers of inflammation (IL-6, TNF-α, CRP) and endothelial function (sICAM-1, sVCAM-1) in serum and saliva, and to assess the relationship between nitrate/nitrite levels and these biomarkers, as well as how OTC mouthwash modulated this relationship. We hypothesize that nitrates/nitrites are associated with these biomarkers, and that their associations would vary with the frequency of mouthwash use.

Method

Our cross-sectional study used data and specimen from the baseline of the San Juan Overweight Adult Longitudinal Study (SOALS). Robust Gamma regression with log-link function, Spearman correlations and partial correlations adjusted for covariates were used for the analysis.

Results

Using OTC mouthwash twice a day or more was significantly associated with lower serum nitrite levels compared to less frequent use (β = −0.357, 95% CI: −0.650, −0.064), but not with other markers of inflammation and endothelial function. Mouthwash use differentially impacted the relationship between nitrate/nitrite and TNF-α, sICAM-1 and sVCAM-1. Specifically, in the participants who used mouthwash less than twice a day or no use, TNF-α (β = −0.35, 95% CI: −0.52, −0.18), and sICAM-1 (β = −0.21, 95% CI: −0.32, −0.09) were negatively associated with serum nitrite. In the participants who used mouthwash twice a day or more use, TNF-α was positively associated with serum nitrate (β = 3.36, 95% CI: 2.07, 4.65), salivary nitrite (β = 1.04, 95% CI: 0.39, 1.69) and salivary nitrate (β = 0.48, 95% CI: 0.25, 0.71); sICAM-1 was positively associated with serum nitrate (β = 1.58, 95% CI: 0.86, 2.29). In both subgroups of mouthwash users, sVCAM-1 was positively correlated with serum nitrate and salivary nitrate. In addition, sVCAM-1 was positively correlated with serum nitrite in participants who used mouthwash frequently (ρ_S = 0.18, p = 0.045).

Discussion

Regular use of OTC mouthwash was associated with systemic nitric oxide. This raises concerns about its potential effects on the levels of inflammatory and endothelial biomarkers associated with cardiometabolic diseases.
Metaverse in industrial contexts - a comprehensive review
Authors: Kour Ravdeep , Karim Ramin , Venkatesh Sridharan Naveen , Kumar Uday
Published: January 2025

This paper explores the potential of Metaverse technology in industrial Asset Management (AM). By integrating AI and digital technologies, the Metaverse can enhance Human-System-Interaction (HSI) and optimise AM processes. However, implementing a Metaverse in industrial contexts faces challenges, particularly in visualising physical and virtual assets. This paper conducts a systematic review to address these challenges and identify potential solutions. The findings reveal that while the necessary technologies are available, their widespread adoption in industrial AM is limited. The paper presents a comprehensive overview of research themes related to Metaverse applications in industrial contexts, highlighting the evolving landscape and potential benefits. Ultimately, this research aims to contribute to the advancement of Metaverse technology in industrial AM by providing insights into its development, implementation, and challenges along with an Industrial Metaverse Framework. An example of applying the Metaverse concept in the railway sector has been presented and validated using railway digital assets available within the eMaintenance LAB. The practical implications of this work are expected to result in increased efficiency and effectiveness in the operation and maintenance procedures across various industrial sectors.
Enhancing augmented reality with machine learning for hands-on origami training
Authors: Łysakowski Mikołaj , Gapsa Jakub , Lyu Chenxu , Bohné Thomas , Tadeja Sławomir Konrad , Skrzypczyński Piotr
Published: January 2025

This research explores integrating augmented reality (AR) with machine learning (ML) to enhance hands-on skill acquisition through origami folding. We developed an AR system using the YOLOv8 model to provide real-time feedback and automatic validation of each folding step, offering step-by-step guidance to users. A novel approach to training dataset preparation was introduced, which improves the accuracy of detecting and assessing origami folding stages. In a formative user study involving 16 participants tasked with folding multiple origami models, the results revealed that while the ML-driven feedback increased task completion times, it also made participants feel more confident throughout the folding process. However, they also reported that the feedback system added cognitive load, slowing their progress, though it provided valuable guidance. These findings suggest that while ML-supported AR systems can enhance the user experience, further optimization is required to streamline the feedback process and improve efficiency in complex manual tasks.
Behavioral analysis of loan decision-making and influencing factors among food-producing new agricultural operating entities in China
Authors: Wei Jingzhou , Fang Taihuang
Published: January 2025

The inherent fragility of the agricultural industry significantly restricts the financing channels available to new agricultural operating entities. Access to credit loans emerges as a pivotal means to address capital shortages among farmers and enhance production inputs. Drawing on survey data from 17,745 new agricultural operating entities engaged in food production in Lu’an City, Anhui Province, and agricultural households documented in the China Household Finance Survey Database, this paper employs the Logit model and the Heckman selection model to empirically analyze the loan decision-making behavior of these entities from two perspectives: loan willingness and credit scale. The research reveals that several key variables exert a significant positive influence on the borrowing willingness of grain producers. Specifically, the planting area range, input range per hectare, the rate range of return on investment, membership in cooperatives, and operation as a family farm all notably enhance their willingness to seek loans. Conversely, the net income per hectare and the number of crop types cultivated significantly diminish their inclination to borrow. Additionally, male operators and those with higher educational backgrounds demonstrate a stronger willingness to obtain loans. Furthermore, the study indicates that the planting area and membership in cooperatives also positively correlate with the scale of loans secured by these agricultural operating entities. Therefore, from the perspective of food security, it is essential to cultivate food-producing new agricultural operating entities. This requires a focus on the counter-cyclical adjustment of financial support, increasing credit support during years of low investment returns. Additionally, it is necessary to develop multiple forms of moderate-scale operations, enhance policy support, and boost the production enthusiasm of food-producing new agricultural operating entities.
Impact of hygienic milk production training on knowledge, attitudes and practices of women farmers in the central highlands of Ethiopia
Authors: Keba Abdi , Tola Alganesh , Kaylegian Kerry E. , Kebede Muluken , Zewdu Ashagrie
Published: January 2025

Introduction

Food safety remains a challenge in developing nations, including Ethiopia, where dairy products are known to be contaminated with foodborne pathogens. Limited food safety interventions studies for improving food safety along the food supply chain have been conducted. The objective of this intervention study was to improve the knowledge, attitudes, and practices (KAPs) of women dairy farmers in the central highlands of Ethiopia by developing and delivering training customized to their education level.

Methods

A total of 120 dairy women farmers were recruited from local milk cooperatives and milk collection centers at four study sites. Training materials on clean milk production and hygienic milking practices were developed for an audience that may not be able to read or write. The 3-day curriculum was delivered using theoretical lecturing, video presentation, practical activities, and handouts, and learning was assessed with a questionnaire and checklist.

Results

The study indicated that women dairy farmers improved their knowledge, attitude, and practices relating to quality milk production. However, milk shade assessments of individual farmers identified many important risk factors for clean milk production that were due to infrastructure issues.

Discussion

The effectiveness of the training materials and approach was supported by the increase in KAPs assessment scores from pre-training to post-training for all study sites. Comparisons of the training outcomes and evaluation results of milk shades were found counterintuitive, which revealed the complexity of food safety interventions. Customized food safety training is effective but should be combined with dairy farming infrastructure improvements to achieve the goal of clean milk production at farm level.