bluuebunny/crossref_metadata_2025 huggingface dataset:
Association between Abnormal Antenatal Doppler Characteristics and Gastrointestinal Outcomes in Preterm Infants
Authors: Martini Silvia , Annunziata Mariarosaria , Della Gatta Anna Nunzia , Aceti Arianna , Brunetti Marica , Pilu Gianluigi , Simonazzi Giuliana , Corvaglia Luigi
Published: December 2022

Antenatal Doppler disturbances are associated with fetal hypoxia and may induce a brain-sparing vascular redistribution at the expense of splanchnic circulation, possibly predisposing to gut complications. We aimed to compare several gastrointestinal outcomes among very-low-birthweight (VLBW) preterm infants with different antenatal Doppler features. VLBW infants born between 2010–2022 were retrospectively included and stratified into the following clusters based on antenatal Doppler characteristics: normal Doppler (controls); absent or reversed end-diastolic flow in the umbilical artery (UA-AREDF) alone or also in the ductus venosus (UA+DV-AREDF); and abnormal Doppler with or without brain-sparing redistribution. The following outcomes were evaluated: time to reach full enteral feeds (FEF), feeding intolerance (FI), necrotizing enterocolitis (NEC), and spontaneous intestinal perforation (SIP). Overall, 570 infants were included. Infants born following UA+DV-AREDF had significantly higher FI, NEC, and SIP rates and achieved FEF later compared to controls. Increased FI prevalence and a longer time to FEF compared to controls were also observed among UA-AREDF infants and in the presence of brain-sparing redistribution, which also increased NEC rates. Antenatal Doppler abnormalities exacerbate the gastrointestinal risks of preterm infants. Detailed knowledge of Doppler features can aid in identifying those at highest risk of intestinal complications who may benefit from tailored enteral feeding management.
Liver-Derived Exosomes Induce Inflammation and Lipogenesis in Mice Fed High-Energy Diets
Authors: Lee Jihee , Jeong Inae , Kim Ok-Kyung
Published: December 2022

The liver is an endocrine organ and is the first organ exposed to nutrients when they are absorbed into the body before being metabolized by the distal organs. Although the liver plays an essential role in the interactions between the metabolic organs, their regulatory mechanisms have not been elucidated. Exosomes mediate communication between cells and primarily enable the transport of lipids, mRNAs, miRNAs, and proteins between cells. In this study, we investigated the effects of lipid metabolism on the liver and adipose tissue between mice fed high-fat (HF) and high-fat/sucrose (HFS) diets and determined the effects of liver tissue-derived exosomes on adipocytes to understand the underlying mechanisms associated with obesity-related metabolic diseases. Normal, HF, and HFS diets were fed to the mice for 12 weeks to compare differences based on dietary patterns. We showed different lipid metabolism effects on the liver and adipose tissue between HF- and HFS-fed mice. In the liver, fibrosis, inflammation, and lipogenesis were activated at higher levels in the HFS than in the HF group, and lipolysis was activated at higher levels in the HF than in the HFS group. In adipose tissue, adipogenesis, fatty acid transport, and lipolysis were activated at higher levels in the HF than in the HFS group, and inflammation and lipogenesis were activated at higher levels in the HFS than in the HF group. This result followed a similar trend reported in 3T3-L1 cells treated with liver-derived exosomes. In addition, the TG content of the liver-derived exosomes was significantly higher, and lipid accumulation was accelerated in the HFS than in the HF group. Based on these results, continuous exposure to HF and HFS diets induces lipid accumulation mediated by liver-derived exosomes; however, there is a difference in lipid metabolism. These results contribute to the elucidation of the mechanisms of exosome function in relation to obesity-related metabolic diseases and the metabolic relationship between tissues.
A Higher Healthy Eating Index Is Associated with Decreased Markers of Inflammation and Lower Odds for Being Overweight/Obese Based on a Case-Control Study
Authors: Vahid Farhad , Jalili Mahsa , Rahmani Wena , Nasiri Zahra , Bohn Torsten
Published: December 2022

Obesity is a health risk characterized by chronic inflammation, and food choices are strongly associated with its etiology. Our objective was to investigate the association between dietary patterns and the healthy eating index (HEI) with the odds of overweight/obesity and related inflammatory markers. Within a population-based case-control study, we collected data and samples from 793 normal-weight and 812 overweight/obese Iranian people (based on either body mass index (BMI) or body surface area (BSA)). Dietary intake and HEI scores were obtained via a validated 124-item food frequency questionnaire. Anthropometric and socioeconomic parameters, as well as blood inflammatory markers, were measured. Participants with higher HEI scores had higher serum high-density lipoprotein-cholesterol (HDL-C) and significantly lower energy intake. Water consumption in the overweight/obese group was significantly lower than in the control group. In the final models using partial correlation and controlling for multiple confounders, there was a significant inverse correlation between HEI and interleukin-4 (IL-4, R = −0.063), IL-1β (R = −0.054), and high-sensitivity C-reactive protein (hs-CRP, R = −0.069). Based on multivariable logistic regression models adjusted for multiple confounders, there was a significant association between HEI as a continuous variable (OR = 0.993, 95% CI: 0.988–0.999) and categorical variable (OR = 0.801, 95% CI: 0.658–0.977) and odds of overweight/obesity across BMI groups. The dietary patterns in the case and control groups however were similar, and we failed to find a significant association between HEI and odds of overweight/obesity based on BSA. Adherence to healthy eating recommendations may be a prudent recommendation to prevent overweight/obesity and keeping inflammatory indicators low.
Association of Dietary Protein Intake with Muscle Mass in Elderly Chinese: A Cross-Sectional Study
Authors: Ouyang Yifei , Huang Feifei , Zhang Xiaofan , Li Li , Zhang Bing , Wang Zhihong , Wang Huijun
Published: December 2022

Most data regarding the association between dietary protein intake and muscle mass come from developed Western countries. This cross-sectional study investigates the association between the amount and distribution of dietary protein intake and muscle mass in elderly Chinese adults. This analysis includes 4826 participants aged 60 years and above from the dataset of the China Health and Nutrition Survey (CHNS) 2018. Protein intake data were assessed using 3-day, 24 h dietary recalls. Appendicular skeletal muscle mass (ASM) was obtained using the bioelectrical impedance analysis (BIA). Two-thirds of dietary protein intake comes from plant sources in the elderly Chinese population. The median total dietary protein intake was 60.5 g/d in low muscle mass participants for males and 52.5 g/d for females, which was lower than for their respective counterparts. Compared to the lowest quartile of protein intake, the highest total protein intake group had increased muscle mass by 0.96 kg among men and by 0.48 kg among women (p < 0.0001), and the highest vegetable protein intake group had increased muscle mass by 0.76 kg among men and by 0.35 kg among women (p < 0.0001). The amount of dietary protein intake with each meal was less than 20 g. High total protein intake and high plant-based protein intake were positively associated with higher muscle mass. A U-shape was observed between total dietary protein intake and low muscle mass risk in elderly Chinese adults. It should be encouraged to increase total daily protein intake to maintain muscle health.
Higher Resistance of Yersinia enterocolitica in Comparison to Yersinia pseudotuberculosis to Antibiotics and Cinnamon, Oregano and Thyme Essential Oils
Authors: Hulankova Radka
Published: December 2022

Yersiniosis is an important zoonotic disease; however, data are scarce on the resistance of enteropathogenic yersiniae, especially that of Y. pseudotuberculosis. Minimum inhibitory concentrations (MIC) of 21 antibiotics and 3 essential oils (EOs) were determined by broth microdilution for Y. enterocolitica bioserotype 4/O:3 strains isolated from domestic swine (n = 132) and Y. pseudotuberculosis strains isolated from wild boars (n = 46). For 15 of 21 antibiotics, statistically significant differences were found between MIC values of Y. enterocolitica and Y. pseudotuberculosis. While Y. enterocolitica was more resistant to amoxiclav, ampicillin, cefotaxime, cefuroxime, gentamicin, imipenem, meropenem, tetracycline, tobramycin, and trimethoprim, Y. pseudotuberculosis was more resistant to cefepime, ceftazidime, colistin, erythromycin, and nitrofurantoin. Statistically significant differences were found between various essential oils (p < 0.001) and species (p < 0.001). The lowest MICs for multiresistant Y. enterocolitica (n = 12) and Y. pseudotuberculosis (n = 12) were obtained for cinnamon (median 414 and 207 μg/mL, respectively) and oregano EOs (median 379 and 284 μg/mL), whereas thyme EO showed significantly higher MIC values (median 738 and 553 μg/mL; p < 0.001). There was no difference between Y. enterocolitica strains of plant (1A) and animal (4/O:3) origin (p = 0.855). The results show that Y. enterocolitica is generally more resistant to antimicrobials than Y. pseudotuberculosis.
Anthelmintic Efficacy in Sheep and Goats under Different Management and Deworming Systems in the Region of Lisbon and Tagus Valley, Portugal
Authors: Antunes Maria Inês , Lima Miguel Saraiva , Stilwell George , Romeiras Maria Inês , Fragoso Luís , Madeira de Carvalho Luís Manuel
Published: December 2022

Infections caused by gastrointestinal parasites have been described worldwide as one of the most important issues impacting small ruminant production. The systematic administration of anthelmintic (AH) drugs without following good practice principles has led to an increase in anthelmintic resistance (AR). There is scarce information regarding AH efficacy in small ruminants in Portugal. This study aimed to characterize by in vivo methods the presence and level of AR in four farms in the region of Lisbon and Tagus Valley. All four farms kept small herds in extensive management systems and used different deworming protocols. The active substances used were fenbendazole and a combination of mebendazole plus closantel in a dosage according to the manufacturers’ instructions. On day 0 (T0), fecal samples were collected from all study animals, and animals in the treatment group (n = 40) were dewormed with the AH previously chosen by the assistant veterinarian. Animals in the control group (n = 30) did not receive any AH drug. The fecal sample collection was repeated on day 15 (T15), and the control group was treated. Egg counts were performed using the McMaster method for the eggs per gram (EPG), and AR was evaluated by the fecal egg count reduction test (FECRT) with a 95% confidence level (CL). The results from this experiment indicated that the four farms presented AR with two farms to fenbendazole (FECRT of 48% and 85%) and two farms to mebendazole plus closantel (FECRT of 66% and 79%). These results indicate that the gastrointestinal parasites of the four studied Portuguese farms are resistant to benzimidazoles, which suggests an increase in AR regarding nematodes in small ruminant production systems in Portugal.
Ten Years of African Swine Fever in Ukraine: An Endemic Form of the Disease in the Wild Boar Population as a Threat to Domestic Pig Production
Authors: Omelchenko Hanna , Avramenko Natalia O. , Petrenko Maksym O. , Wojciechowski Jarosław , Pejsak Zygmunt , Woźniakowski Grzegorz
Published: December 2022

(1) Background: African swine fever (ASF) has been present in Ukraine for more than ten years (2012–2022). The purpose of our study was to perform a retrospective analysis of the spread of ASF to assess the role of wild boar in the epizootic expansion in Ukraine. (2) Methods: Statistical materials were collected and the epizootic situation of ASF from 2012 to 2022 was examined. The potential sources of the African swine fever virus (ASFV) and transmission factors were analysed. The main factors exerting negative impacts on domestic pig production were also analysed. (3) Results: Consequently, from the results of the retrospective analysis of ASF outbreaks in Ukraine, the probability ratio of ASF outbreaks in the wild boar and domestic pig populations was determined. The data show a direct relationship between ASF outbreaks among wild boar and domestic pigs with the observed decay of wild boar outbreaks across the entire territory of Ukraine. At the same time, an increase in the number of wild boars has been observed in the Mykolaiv region, with a parallel spillover of outbreaks in domestic pigs. (4) Conclusions: The epidemiological situation observed for ASF in the wild boar population may suggest an endemic form of the disease. This may further complicate eradication programs and the protection of domestic pig farms from ASF outbreaks. An additional and major reason to control the ASF epizootic is the continuing military Russian offensive in Ukraine.
Small Animal In Situ Drug Delivery Effects via Transdermal Microneedles Array versus Intravenous Injection: A Pilot Observation Based on Photoacoustic Tomography
Authors: Zhou Yingying , Huang Xiazi , Li Jiyu , Zhu Ting , Pang Weiran , Chow Larry , Nie Liming , Sun Lei , Lai Puxiang
Published: December 2022

Intravenous injection is a rapid, low-cost, and direct method that is commonly used to deliver multifarious biotherapeutics and vaccines. However, intravenous injection often causes trauma or tissue injury that requires professional operation. Transdermal drug delivery overcomes the aforementioned defects, and the microneedles (MNs) array is one of the most promising transdermal drug delivery platforms. Timely, precise, and non-invasive monitoring and evaluation of the effects of MNs in transdermal administration is significant to the research of drug efficiency response to specific diseases. In this sense, photoacoustic computed tomography (PACT), which provides wavelength-selective and deep-penetrating optical contrast, could be a promising imaging tool for in situ evaluation of the treatment effects. In this work, we propose the use of PACT to non-invasively assess the effects of real-time drug delivery in glioma tumors through transdermal administration with degradable indocyanine green-loaded hyaluronic acid MNs (ICG-HA-MNs). The outcome is systematically and quantitatively compared with that via intravenous injection. It is found that the photoacoustic signals of ICG in the tumor site express a faster elevation and shorter duration time in the intravenous injection group; by contrast, the photoacoustic signals demonstrate a lower intensity but prolonged duration time in the MNs group. The observed phenomenon indicates faster response but shorter drug duration for intravenous injection, which is in contrast with the lower loading but prolonged performance for transdermal drug delivery with MNs. These results exhibit good consistency with the earlier, common-sense findings reported from other aspects, confirming that PACT can serve as a potential imaging tool to precisely, non-invasively, and quickly evaluate in situ drug delivery effects and provide constructive guidance for the design and fabrication of microneedles.
Superparamagnetic Iron Oxide Nanoparticles and Curcumin Equally Promote Neuronal Branching Morphogenesis in the Absence of Nerve Growth Factor in PC12 Cells
Authors: Zarei Mahshid , Esmaeili Abolghasem , Zarrabi Ali , Zarepour Atefeh
Published: December 2022

Regeneration of the damaged neurons in neurological disorders and returning their activities are two of the main purposes of neuromedicine. Combination use of specific nanoformulations with a therapeutic compound could be a good candidate for neuroregeneration applications. Accordingly, this research aims to utilize the combination of curcumin, as a neurogenesis agent, with dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) to evaluate their effects on PC12 cellsʹ neuronal branching morphogenesis in the absence of nerve growth factor. Therefore, the effects of each component alone and in combination form on the cytotoxicity, neurogenesis, and neural branching morphogenesis were evaluated using MTT assay, immunofluorescence staining, and inverted microscopy, respectively. Results confirmed the effectiveness of the biocompatible iron oxide nanoparticles (with a size of about 100 nm) in improving the percentage of neural branching (p < 0.01) in PC12 cells. In addition, the combination use of these nanoparticles with curcumin could enhance the effect of curcumin on neurogenesis (p < 0.01). These results suggest that SPIONs in combination with curcumin could act as an inducing factor on PC12 neurogenesis in the absence of nerve growth factor and could offer a novel therapeutic approach to the treatment of neurodegenerative diseases.
A Review of 3D-Printing of Microneedles
Authors: Olowe Michael , Parupelli Santosh Kumar , Desai Salil
Published: December 2022

Microneedles are micron-sized devices that are used for the transdermal administration of a wide range of active pharmaceutics substances with minimally invasive pain. In the past decade, various additive manufacturing technologies have been used for the fabrication of microneedles; however, they have limitations due to material compatibility and bioavailability and are time-consuming and expensive processes. Additive manufacturing (AM), which is popularly known as 3D-printing, is an innovative technology that builds three-dimensional solid objects (3D). This article provides a comprehensive review of the different 3D-printing technologies that have the potential to revolutionize the manufacturing of microneedles. The application of 3D-printed microneedles in various fields, such as drug delivery, vaccine delivery, cosmetics, therapy, tissue engineering, and diagnostics, are presented. This review also enumerates the challenges that are posed by the 3D-printing technologies, including the manufacturing cost, which limits its viability for large-scale production, the compatibility of the microneedle-based materials with human cells, and concerns around the efficient administration of large dosages of loaded microneedles. Furthermore, the optimization of microneedle design parameters and features for the best printing outcomes is of paramount interest. The Food and Drug Administration (FDA) regulatory guidelines relating to the safe use of microneedle devices are outlined. Finally, this review delineates the implementation of futuristic technologies, such as artificial intelligence algorithms, for 3D-printed microneedles and 4D-printing capabilities.
Synthesis and Cytotoxicity Assessment of Citrate-Coated Calcium and Manganese Ferrite Nanoparticles for Magnetic Hyperthermia
Authors: Andrade Raquel G. D. , Ferreira Débora , Veloso Sérgio R. S. , Santos-Pereira Cátia , Castanheira Elisabete M. S. , Côrte-Real Manuela , Rodrigues Ligia R.
Published: December 2022

Calcium-doped manganese ferrite nanoparticles (NPs) are gaining special interest in the biomedical field due to their lower cytotoxicity compared with other ferrites, and the fact that they have improved magnetic properties. Magnetic hyperthermia (MH) is an alternative cancer treatment, in which magnetic nanoparticles promote local heating that can lead to the apoptosis of cancer cells. In this work, manganese/calcium ferrite NPs coated with citrate (CaxMn1−xFe2O4 (x = 0, 0.2, 1), were synthesized by the sol-gel method, followed by calcination, and then characterized regarding their crystalline structure (by X-ray diffraction, XRD), size and shape (by Transmission Electron Microscopy, TEM), hydrodynamic size and zeta potential (by Dynamic Light Scattering, DLS), and heating efficiency (measuring the Specific Absorption Rate, SAR, and Intrinsic Loss Power, ILP) under an alternating magnetic field. The obtained NPs showed a particle size within the range of 10 nm to 20 nm (by TEM) with a spherical or cubic shape. Ca0.2Mn0.8Fe2O4 NPs exhibited the highest SAR value of 36.3 W/g at the lowest field frequency tested, and achieved a temperature variation of ~7 °C in 120 s, meaning that these NPs are suitable magnetic hyperthermia agents. In vitro cellular internalization and cytotoxicity experiments, performed using the human cell line HEK 293T, confirmed cytocompatibility over 0–250 µg/mL range and successful internalization after 24 h. Based on these studies, our data suggest that these manganese-calcium ferrite NPs have potential for MH application and further use in in vivo systems.
How Carbon Nanoparticles, Arbuscular Mycorrhiza, and Compost Mitigate Drought Stress in Maize Plant: A Growth and Biochemical Study
Authors: Alsherif Emad A. , Almaghrabi Omar , Elazzazy Ahmed M. , Abdel-Mawgoud Mohamed , Beemster Gerrit T. S. , Sobrinho Renato Lustosa , AbdElgawad Hamada
Published: December 2022

Drought negatively affects crop growth and development, so it is crucial to develop practical ways to reduce these consequences of water scarcity. The effect of the interactive potential of compost (Comp), mycorrhizal fungi (AMF), and carbon nanoparticles (CNPS) on plant growth, photosynthesis rate, primary metabolism, and secondary metabolism was studied as a novel approach to mitigating drought stress in maize plants. Drought stress significantly reduced maize growth and photosynthesis and altered metabolism. Here, the combined treatments Com-AMF or Com-AMF-CNPs mitigated drought-induced reductions in fresh and dry weights. The treatments with AMF or CNPS significantly increased photosynthesis (by 10%) in comparison to the control plants. Results show that soluble sugars were accumulated to maintain the osmotic status of the maize plant under drought stress. The level and metabolism of sucrose, an osmo-protectant, were increased in plants treated with Com (by 30%), which was further increased under the triple effect of Com-AMF-CNPs (40%), compared to untreated plants. This was inconsistent with increased sucrose-phosphate synthase and sucrose-P-synthase activity. The combined treatment Com-AMF-CNPs increased the levels of oxalic and succinic acids (by 100%) and has been reflected in the enhanced levels of amino acids such as the antioxidant and omso-protectant proline. Higher increases in fatty acids by treatment with CNPS were also recorded. Com-AMF-CNPs enhanced many of the detected fatty acids such as myristic, palmitic, arachidic, docosanoic, and pentacosanoic (110%, 30%, 100%, and 130%, respectively), compared to untreated plants. At the secondary metabolism level, sugar and amino acids provide a route for polyamine biosynthesis, where Com-AMF-CNPs increased spermine and spermidine synthases, ornithine decarboxylase, and adenosyl methionine decarboxylase in treated maize. Overall, our research revealed for the first time how Cmo, AMF, and/or CNPS alleviated drought stress in maize plants.
Diversity and Differentiation of Duckweed Species from Israel
Authors: Friedjung Yosef Avital , Ghazaryan Lusine , Klamann Linda , Kaufman Katherine Sarah , Baubin Capucine , Poodiack Ben , Ran Noya , Gabay Talia , Didi-Cohen Shoshana , Bog Manuela , Khozin-Goldberg Inna , Gillor Osnat
Published: December 2022

Duckweeds (Lemnaceae) are tiny plants that float on aquatic surfaces and are typically isolated from temperate and equatorial regions. Yet, duckweed diversity in Mediterranean and arid regions has been seldom explored. To address this gap in knowledge, we surveyed duckweed diversity in Israel, an ecological junction between Mediterranean and arid climates. We searched for duckweeds in the north and center of Israel on the surface of streams, ponds and waterholes. We collected and isolated 27 duckweeds and characterized their morphology, molecular barcodes (atpF-atpH and psbK-psbI) and biochemical features (protein content and fatty acids composition). Six species were identified—Lemna minor, L. gibba and Wolffia arrhiza dominated the duckweed populations, and together with past sightings, are suggested to be native to Israel. The fatty acid profiles and protein content further suggest that diverged functions have attributed to different haplotypes among the identified species. Spirodela polyrhiza, W. globosa and L. minuta were also identified but were rarer. S. polyrhiza was previously reported in our region, thus, its current low abundance should be revisited. However, L. minuta and W. globosa are native to America and Far East Asia, respectively, and are invasive in Europe. We hypothesize that they may be invasive species to our region as well, carried by migratory birds that disperse them through their migration routes. This study indicates that the duckweed population in Israel’s aquatic environments consists of both native and transient species.
Aqueous Extract of Artemisia annua Shows In Vitro Antimicrobial Activity and an In Vivo Chemopreventive Effect in a Small-Cell Lung Cancer Model
Authors: Allemailem Khaled S.
Published: December 2022

Artemisia annua (A. annua) has been used as a medicinal plant in the treatment of several infectious and non-infectious diseases in the forms of tea and press juice since ancient times. The aim of this study was to evaluate the aqueous extract of A. annua (AAE) as an antimicrobial agent in vitro and to evaluate its chemopreventive efficacy in vivo in a small-cell lung cancer (SCLC) animal model. The dried powder of AAE was prepared using the Soxhlet extraction system from the leaves of Artemisia annua. The in vitro activity of AAE was determined against Candida albicans (C. albicans), Enterococcus faecalis (E. faecalis), Klebsiella pneumoniae (K. pneumoniae), and methicillin-resistant Staphylococcus aureus (MRSA) using the agar well diffusion method and propidium iodide (PI)-stained microbial death under a confocal microscope. The pretreatment of mice with AAE was initiated two weeks before the first dose of benzo[a]pyrene and continued for 21 weeks. The chemopreventive potential of the extract was evaluated by flow cytometry and biochemical and histopathological analyses of the tissues and serum accordingly, after sacrificing the mice. The data revealed the antimicrobial potential of AAE against all the species investigated, as it showed growth-inhibitory activity by MIC, as well as confocal microscopy. The pretreatment of AAE exhibited significant protection in carcinogen-modulated, average body weight (ABW), and relative organ weight (ROW) cancer biomarkers in the serum and antioxidants in the lungs. The hematoxylin and eosin (H&E) staining of the tissues revealed that AAE prevented malignancy in the lungs. AAE also induced apoptosis and decreased intracellular reactive oxygen species (ROS) in the lung cells analyzed by flow cytometry. The current findings demonstrated the use of AAE as an alternative medicine in the treatment of infectious disease and the chemoprevention of lung cancer. To our knowledge, this is the first study that summarizes the chemopreventive potential of AAE in a lung cancer model in vivo. However, further investigations are suggested to understand the role of AAE to potentiate the therapeutic index of the commercially available drugs that show multiple drug resistance against microbial growth and high toxicity during cancer chemotherapy.
Maltodextrin-Coated Peppermint and Caraway Essential Oils Effects on Soil Microbiota
Authors: Chmiel Maria , Drzymała Gabriela , Bocianowski Jan , Komnenić Andreja , Baran Agnieszka , Synowiec Agnieszka
Published: December 2022

Essential oils exhibit strong antimicrobial effects that can serve as a substitute for synthetic pesticides. However, many reports mention the use of essential oils in protecting above-ground plant organs and storing raw materials and seeds, but only a few address the effects of treatments on soil microbiota. Regarding this, it is necessary to find a solution that will prevent the rapid degradation of oils in soil and extend the period of their action on the soil microbiota. The solution to this problem can be microencapsulation, where the choice of carrier plays a key role. In our experiment, maltodextrin was studied, often used in the microencapsulation of essential oils. It was examined independently in two doses (M1 and M2, with 50 and 200 g kg−1, respectively) and a combination with two essential oils known for their antimicrobial activity. We hypothesized that the selected microbial communities would react differently to the stress caused by maltodextrin-encapsulated essential oils. The serial dilution method assessed the number of colony-forming units (CFU) of bacteria, fungi, and actinomycetes. As the goal of microencapsulation was to prolong the effect of essential oils, their reaction was observed over a longer period. The soil microbial populations were examined in sandy and loamy soil at 1, 7, 14, and 78 days after encapsulated essential oils were mixed with the soil samples. In both types of soil, a significant increase in bacteria and actinomycetes was observed with maltodextrin in both doses. Encapsulated peppermint and caraway oils had different effects on microbes, both inhibitory and stimulatory. It is also important to note that peppermint with a smaller dose of maltodextrin significantly inhibited the growth of fungi in sandy soil in all measurements, as well as that caraway oil with a higher dose of maltodextrin significantly stimulated the growth of bacteria and actinomycetes in sandy soil. The higher dose of maltodextrin could explain this stimulation. Further research is recommended to test different doses of essential oils and maltodextrin, which would lead to the optimal dose of both wall and core materials.
Recent Progress on Tailoring the Biomass-Derived Cellulose Hybrid Composite Photocatalysts
Authors: Chai Yi Ding , Pang Yean Ling , Lim Steven , Chong Woon Chan , Lai Chin Wei , Abdullah Ahmad Zuhairi
Published: December 2022

Biomass-derived cellulose hybrid composite materials are promising for application in the field of photocatalysis due to their excellent properties. The excellent properties between biomass-derived cellulose and photocatalyst materials was induced by biocompatibility and high hydrophilicity of the cellulose components. Biomass-derived cellulose exhibited huge amount of electron-rich hydroxyl group which could promote superior interaction with the photocatalyst. Hence, the original sources and types of cellulose, synthesizing methods, and fabrication cellulose composites together with applications are reviewed in this paper. Different types of biomasses such as biochar, activated carbon (AC), cellulose, chitosan, and chitin were discussed. Cellulose is categorized as plant cellulose, bacterial cellulose, algae cellulose, and tunicate cellulose. The extraction and purification steps of cellulose were explained in detail. Next, the common photocatalyst nanomaterials including titanium dioxide (TiO2), zinc oxide (ZnO), graphitic carbon nitride (g-C3N4), and graphene, were introduced based on their distinct structures, advantages, and limitations in water treatment applications. The synthesizing method of TiO2-based photocatalyst includes hydrothermal synthesis, sol-gel synthesis, and chemical vapor deposition synthesis. Different synthesizing methods contribute toward different TiO2 forms in terms of structural phases and surface morphology. The fabrication and performance of cellulose composite catalysts give readers a better understanding of the incorporation of cellulose in the development of sustainable and robust photocatalysts. The modifications including metal doping, non-metal doping, and metal–organic frameworks (MOFs) showed improvements on the degradation performance of cellulose composite catalysts. The information and evidence on the fabrication techniques of biomass-derived cellulose hybrid photocatalyst and its recent application in the field of water treatment were reviewed thoroughly in this review paper.
MXene/Gelatin/Polyacrylamide Nanocomposite Double Network Hydrogel with Improved Mechanical and Photothermal Properties
Authors: Zhang Zeyu , Hu Yang , Ma Huiling , Wang Yicheng , Zhong Shouchao , Sheng Lang , Li Xiang , Peng Jing , Li Jiuqiang , Zhai Maolin
Published: December 2022

The development of smart hydrogel with excellent mechanical properties and photothermal conversion capability is helpful in expending its application fields. Herein, a MXene/gelatin/polyacrylamide (M/G/PAM) nanocomposite double network (NDN) hydrogel was synthesized by γ-ray radiation technology for the first time. Compared with gelatin/polyacrylamide double network hydrogel, the optimized resultant M3/G/PAM NDN hydrogel shows better mechanical properties (tensile strength of 634 ± 10 kPa, compressive strength of 3.44 ± 0.12 MPa at a compression ratio of 90%). The M3/G/PAM NDN hydrogel exhibits a faster heating rate of 30 °C min−1, stable photothermal ability, and mechanical properties even after 20 cycles of on–off 808 nm near-infrared (NIR) laser irradiation (1.0 W cm−2). Furthermore, the temperature of M3/G/PAM NDN hydrogel can be increased rapidly from 25 °C to 90 °C in 10 s and could reach 145 °C in 120 s under irradiation by focused NIR laser irradiation (56.6 W cm−2). The high mechanical property and photothermal properties of M/G/PAM hydrogel are ascribed to the formation of double network and uniform hydrogen bonding between MXene and gelatin and PAM polymers. This work paves the way for construction of photothermal hydrogels with excellent mechanical properties.
Recent Developments on Bioinspired Cellulose Containing Polymer Nanocomposite Cation and Anion Exchange Membranes for Fuel Cells (PEMFC and AFC)
Authors: Thangarasu Sadhasivam , Oh Tae-Hwan
Published: December 2022

Hydrogen fuel cell (FC) technologies are being worked on as a possible replacement for fossil fuels because they produce a lot of energy and do not pollute the air. In FC, ion-exchange membranes (IEMs) are the vital components for ion transport between two porous electrodes. However, the high production cost of commercialized membranes limits their benefits. Various research has focused on cellulose-based membranes such as IEM with high proton conductivity, and mechanical, chemical, and thermal stabilities to replace the high cost of synthetic polymer materials. In this review, we focus on and explain the recent progress (from 2018 to 2022) of cellulose-containing hybrid membranes as cation exchange membranes (CEM) and anion exchange membranes (AEM) for proton exchange membrane fuel cells (PEMFC) and alkaline fuel cells (AFC). In this account, we focused primarily on the effect of cellulose materials in various membranes on the functional properties of various polymer membranes. The development of hybrid membranes with cellulose for PEMFC and AFC has been classified based on the combination of other polymers and materials. For PEMFC, the sections are associated with cellulose with Nafion, polyaryletherketone, various polymeric materials, ionic liquid, inorganic fillers, and natural materials. Moreover, the cellulose-containing AEM for AFC has been summarized in detail. Furthermore, this review explains the significance of cellulose and cellulose derivative-modified membranes during fuel cell performance. Notably, this review shows the vital information needed to improve the ion exchange membrane in PEMFC and AFC technologies.
In-Situ Assembly of MoS2 Nanostructures on EHD-Printed Microscale PVDF Fibrous Films for Potential Energy Storage Applications
Authors: Zhang Bing , Li Shikang , Qureshi M. Shafin. H. , Mia Ukil , Ge Zhenghui , Song Aiping
Published: December 2022

Three-dimensional (3D) printing has been widely utilized to fabricate free-standing electrodes in energy-related fields. In terms of fabrication, the two most challenging limitations of 3D printed electrodes are the poor printing resolution and simple structural dimension. Here we proposed a novel process to fabricate molybdenum disulfide-polyvinylidene fluoride (MoS2-PVDF) hierarchical electrodes for energy storage applications. The 20-layer microscale PVDF films with a stable fiber width of 8.3 ± 1.2 μm were fabricated by using electrohydrodynamic (EHD) printing. MoS2 nanostructures were synthesized and assembled on the microscale PVDF fibers by using hydrothermal crystal growth. The structural and material investigations were conducted to demonstrate the geometrical morphology and materials component of the composite structure. The electrochemical measurements indicated that the MoS2-PVDF electrodes exhibited the typical charge-discharge performance with a mass specific capacitance of 60.2 ± 4.5 F/g. The proposed method offers a facile and scalable approach for the fabrication of high-resolution electrodes, which might be further developed with enhanced specific capacitance in energy storage fields.
Rock Reinforcement by Stepwise Injection of Two-Component Silicate Resin
Authors: Shilova Tatiana , Serdyukov Aleksander , Serdyukov Sergey , Ivanova Oksana
Published: December 2022

Our research aims to improve the efficiency of the reinforcement of loose rocks with two-component polymer resins. The standard approach consists of the injection of two pre-mixed components into a rock massive. We propose a stepwise injection of individual components of a resin into the rock and deep extrusion of the solutions into the rock by gas between the injection stages. The experimental results indicate that the proposed method provides a reduction of polymer consumption per unit volume of the rock, and an increase in the impregnation depth, area of the resin impact, and the reinforced rock volume in comparison with the conventional method of prepared resin solution injection. The cured resin partially fills the sand rock pore space, binds the grains, and acts as a reinforcing frame. The highest reinforcement is achieved with the sequential stepwise injection of the resin by separate small portions of each component. We have shown the uniaxial compressive strength is on average more than twice as high that obtained with the conventional injection method. This can be explained by higher fracture toughness of the reinforced rock with a flexible hardened network of the cured resin in the structure.
Effect of Tea Polyphenols on the Melt Grafting of Glycidyl Methacrylate onto Polypropylene
Authors: Zheng Xin , He Lina , Yu Guipeng , Li Yongjin
Published: December 2022

It is considered to be one of the most effective strategies to prepare functionalized polypropylene (PP) materials via the melt grafting of polar monomers onto PP chains. However, the grafting efficiency of functional monomers is generally low. To achieve a high grafting efficiency, we explored the effect of tea polyphenols (C), which are good free radical scavengers, on the melt grafting of glycidyl methacrylate (GMA) onto PP chains initiated by dicumyl peroxide (DCP). Specifically, 0.5~3 wt% of tea polyphenols (C) were introduced to the PP/DCP/GMA melt blending system. The morphology, melt flow rate (MFR), thermal and mechanical properties of tea polyphenols (C) incorporated PP/DCP/GMA blends were investigated systematically. The results showed that the proper amount of tea polyphenols (C) (0.5~2 wt%) promoted the grafting of GMA. Unexpectedly, the PP backbone suffered from more severe degradation with the addition of tea polyphenols (C). The phenomena were ascribed to the reaction between phenolic hydroxyl groups of tea polyphenols (C) and epoxy groups of grafted GMA, which was revealed by the FTIR results. In addition, according to DSC and the tensile test, the co-grafting of GMA and tea polyphenols (C) improved the crystallization ability, yield strength and Young’s modulus of the PP matrix.
Self-Assembled Serpentine Ni3Si2O5(OH)4 Hybrid Sheets with Ammonium Polyphosphate for Fire Safety Enhancement of Polylactide Composites
Authors: Yi Xiaohong , Huang Jingshu , Tong Yizhang , Zhao Hui , Cao Xianwu , Wu Wei
Published: December 2022

Biodegradable polylactide (PLA) has been widely utilized in people’s daily lives. In order to improve the fire safety of PLA, ammonium polyphosphate (APP) was self-assembled onto the surface of serpentine Ni3Si2O5(OH)4 through the electrostatic method, followed by mixing with PLA by melt compounding. The APP-modified serpentine (serpentine@APP) dispersed uniformly in the PLA matrix. Compared with pure PLA, the PLA composite with 2 wt% serpentine@APP reduced the peak heat release rate (pHRR) and total heat release (THR) by 43.9% and 16.3%, respectively. The combination of APP and serpentine exhibited suitable synergistic flame-retardant effects on the fire safety enhancement of PLA. In addition, the dynamical rheological tests revealed that the presence of APP and serpentine could reduce the viscosity of PLA composites. The plasticizing effects of APP and serpentine benefited the processing of PLA. The mechanical properties of PLA/serpentine@APP maintained suitable performance as pure PLA. This study provided a feasible way to enhance the fire safety of PLA without sacrificing its mechanical properties.
Effect of Thermal and Hydrothermal Accelerated Aging on 3D Printed Polylactic Acid
Authors: Bergaliyeva Saltanat , Sales David L. , Delgado Francisco J. , Bolegenova Saltanat , Molina Sergio I.
Published: December 2022

In the new transformation of ‘Industry 4.0’, additive manufacturing technologies have become one of the fastest developed industries, with polylactic acid (PLA) playing a significant role. However, there is an increasing amount of garbage generated during the printing process and after prototypes or end-of-life parts. Re-3D printing is one way to recycle PLA waste from fused filament fabrication. To do this process successfully, the properties of the waste mixture should be known. Previous studies have found that PLA degrades hydrolytically, but the time at which this process occurs for 3D printed products is not specified. This work aims to establish the baseline of the degradation kinetics of 3D printed PLA products to predict the service time until which these properties are retained. To achieve this, 3D printed specimens were thermally and hydrothermally aged during several time intervals. Thermal and mechanical properties were also determined. This study reveals that tensile strength decreases after 1344 h of hydrothermal ageing, simulating 1.5–2.5 years of real service time. PLA therefore has the same thermo-mechanical properties before reaching 1.5-years of age, so it could be recycled.
Experimental Characterisation and Finite Element Modelling of Polyamide-12 Fabricated via Multi Jet Fusion
Authors: Lee Kok Peng Marcian , Kajtaz Mladenko
Published: December 2022

The HP Multi Jet Fusion (MJF) technology is a relatively recent addition to powder bed fusion additive manufacturing (AM) techniques. It differentiates itself from selective laser sintering (SLS) technology through the use of fusing and detailing agents to control part geometry, and the use of a planar infrared radiation (IR) source that sweeps over the powder bed to initiate the sintering process. Depending on the printing methodology, AM processes can introduce mechanical property anisotropy that is dependent on print orientation. In the case of MJF-fabricated parts, there is a general disagreement over the influence of print orientation on tensile mechanical properties in the literature. In this work, MJF-fabricated PA12 (AM PA12) is printed at various orientations and characterised in terms of tensile and compressive mechanical properties. The orientations have been selected to take into account the alignment of the IR source sweep direction to the test load. We observe that orientating parts towards the vertical direction for printing tends to favour enhanced tensile mechanical properties. The anisotropy in mechanical properties is attributed to more complete polymer powder fusion as a result of the increased number of IR source sweeps when parts are orientated towards the vertical direction. Both tensile and compressive stress–strain data were used as experimental data input for calibrating the Elastic–Plastic with combined hardening (EPC) material model in the commercial finite element analysis (FEA) package—Abaqus. We demonstrate that the EPC material is a suitable material model for the FEA of AM PA12.
Recent Developments in Inorganic Composites in Rotational Molding
Authors: Ortega Zaida , McCourt Mark , Romero Francisco , Suárez Luis , Cunningham Eoin
Published: December 2022

Rotational molding allows for obtaining hollow parts with good aesthetics and properties, having as main drawbacks the lack of pressure and the long cycle times, which limit the range of materials. Different fillers have been introduced in rotomolding to obtain composite materials assessed. This review has shown that glass fibers or particles are the most common material among them, although carbon fibers or clays have also been studied. In general terms, 10% loadings provide an increase in mechanical properties; higher loadings usually lead to a decrease in processability or final properties. When the filler consists of a micro- or nano-material, such as clay or graphene, lower loadings are proposed, generally not exceeding 3%. The use of fillers of an inorganic nature to obtain composites has not been as explored as the incorporation of lignocellulosic materials and even less if referring to waste materials or side streams from industrial processes. So, there is a broad field for assessing the processing and properties of rotomolded composites containing inorganic waste materials, including the study of the relationship between the ratio of filler/reinforcement and the final properties and also their preprocessing (dry blending vs. melting compounding).
Stability Analysis of a Chemostat Model for Phenol and Sodium Salicylate Mixture Biodegradation
Authors: Borisov Milen , Dimitrova Neli , Zlateva Plamena
Published: December 2022

In this paper we consider a mathematical continuous-time model for biodegradation of phenol in the presence of sodium salicylate in a chemostat. The model is described by a system of three nonlinear ordinary differential equations. Based on the dynamical systems theory we provide mathematical investigations of the model including local and global analysis of the solutions. The local analysis consist in computation of two equilibrium points—one interior and one boundary (washout) equilibrium—in dependance of the dilution rate as a key model parameter. The local asymptotic stability of the equilibria is also presented. The global analysis of the model solutions comprises proving existence, uniqueness and uniform boundedness of positive solutions, as well as global asymptotic stabilizability of the dynamics. The theoretical investigations are illustrated by some numerical examples. The results in this study can be used in practice as a tool to control and optimize the chemostat performance of simultaneous biodegradation of mixed substrates in wastewater.