bluuebunny/crossref_metadata_2025 huggingface dataset:
Greenhouse Gas Emissions from Beef Cattle Breeding Based on the Ecological Cycle Model
Authors: Guo Hongpeng , Su Zixu , Yang Xiao , Xu Shuang , Pan Hong
Published: August 2022

Over the past few decades, the supply of beef has increasingly become available with the great improvement of the quality of life, especially in developing countries. However, along with the demand for meat products of high quality and the transformation of dietary structure, the impact of massive agricultural greenhouse gas emissions on the environmental load cannot be ignored. Therefore, the objective of this study is to predict the annual greenhouse gas emissions of 10 million heads of beef cattle under both the ecological cycle model (EC model) and the non-ecological cycle model (non-EC model), respectively, in order to compare the differences between these two production models in each process, and thus explore which one is more sustainable and environmentally friendly. To this end, through the life cycle assessment (LCA), this paper performs relevant calculations according to the methodology of 2019 Refinement to the 2006 IPCC Guidelines for National Greenhouse Gas Inventories (2019 IPCC Inventories). The results have shown that the total GHG emissions of the non-EC model were almost 4 times higher than those of the EC model, and feed-grain cultivation and manure management were main emission sources in both models. The non-EC model produced significantly more emissions than the EC model in each kind of GHG, especially the largest gap between these two was in CO2 emissions that accounted for 68.01% and 56.17% of the respective planting and breeding systems. This study demonstrates that the transformation of a beef cattle breeding model has a significant direct impact on cutting agricultural GHG emissions, and persuades other countries in the similar situation to vigorously advocate ecological cycling breeding model instead of the traditional ones so that promotes coordinated development between planting industry and beef cattle breeding industry.
Exploration of Spatio-Temporal Characteristics of Carbon Emissions from Energy Consumption and Their Driving Factors: A Case Analysis of the Yangtze River Delta, China
Authors: Wang Weiwu , Chen Huan , Wang Lizhong , Li Xinyu , Mao Danyi , Wang Shan
Published: August 2022

For the Yangtze River Delta (YRD) region of China, exploring the spatio-temporal characteristics of carbon emissions from energy consumption (CEECs) and their influencing factors is crucial to achieving carbon peaking and carbon neutrality as soon as possible. In this study, an improved LMDI decomposition model based on the Tapio model and Kaya’s equation was proposed. Combined with the improved LMDI and k-means cluster analysis methods, the energy structure, energy intensity, unit industrial output value and population size were selected as the driving factors, and the contribution of each driving factor to the CEECs of prefecture-level cities was quantitatively analyzed. Our study found that: (1) By 2020, the total amount of CEECs in the 26 prefecture-level cities in the YRD will stabilize, while their intensity has shown a downward trend in recent years. (2) The decoupling relationship between CEECs and economic development generally showed a trend from negative decoupling to decoupling. The dominant factor in decoupling was generally the shift of DEL values towards urbanization rate and energy intensity and the open utilization of energy technologies. (3) From 2000 to 2010, the dominant factors affecting CEECs in 26 cities were energy intensity and energy structure, followed by industrial output value and urbanization rate. In general, the promotion effect of economic development on carbon emissions in the YRD region was greater than the inhibitory effect. After 2010, the restrictive effect of various factors on CEECs increased significantly, among which the role of gross industrial output was crucial. The research results can provide a scientific policy basis for the subsequent spatial management and control of carbon emission reduction and carbon neutrality in the YRD region at a finer scale.
The Influence of a Cold Front and Topography on the Initiation and Maintenance of a Precipitation Convective System in North China: A Case Study
Authors: Li Yan , Wang Yu , Chen Xianyan
Published: August 2022

By using the convection-resolving weather research and forecasting simulation, a convective rainfall event over the middle portions of the eastern foothills of the Taihang Mountains in North China is investigated in this study. The influences of the cold front and complex topography on the initiation and maintenance of the convective system are analyzed. Results show two reasons why the convective clusters are initiated near noon on the hillsides at an elevation of 800 m. First, a local topographic convergence zone usually appears on the eastern slope of the Taihang Mountains near noon in May. Second, such a topographic convergence zone is enhanced by a cold front system and then triggers the convective clusters. Subsequently, the convective cells strengthen when moving downslope and weaken when moving eastward on the plain. When moving downslope, the atmospheric stratification is convectively unstable, and the mountain–plains solenoid (MPS) is strong near the foot of the mountain. The large amount of water vapor carried by the MPS-induced easterly wind is forced to ascend by topographic obstructions, and therefore the convective cells develop. As a result, heavy rainfall occurs on the hillsides with an elevation of 200–600 m. When the convective cells move eastward on the plain, the atmospheric stratification is stable, and the MPS is weak. Thus, convective activities weaken. Moreover, the results reveal that the mesoscale convergence line, slope gradient and slope aspect of the local terrain, local atmospheric instability, and the MPS play different roles in maintaining the convective system at elevations of 200–600 m along the eastern foothills of the Taihang Mountains.
Relationship between Psychological Stress Determined by Voice Analysis and Periodontal Status: A Cohort Study
Authors: Maruyama Takayuki , Ekuni Daisuke , Higuchi Masakazu , Takayama Eiji , Tokuno Shinichi , Morita Manabu
Published: August 2022

In modern society, evaluation and management of psychological stress may be important for the prevention of periodontal disease. The purpose of this study was to examine the relationship between psychological stress (vitality and mental activity) evaluated by Mind Monitoring System (MIMOSYS) and periodontal status. Forty students of Okayama University underwent the oral examination and self-reported questionnaire on the first day (baseline) and the 14th day (follow-up). Voice recording was performed every day with the MIMOSYS app during the whole study period. The participants completed the Patient Health Questionnaire (PHQ)-9 and Beck Depression Inventory (BDI) at baseline and at follow-up. Spearman’s rank correlation coefficient was used to determine the significance of correlations among variables. The PHQ-9 and BDI scores were negatively correlated with vitality in the morning. Change in vitality in the morning was significantly correlated with changes in periodontal inflammation. Mental activity was significantly correlated with change in mean probing pocket depth. This result shows that measurement of psychological stress using a voice-based tool to assess mental health may contribute to the early detection of periodontal disease.
The Long Goodbye: Finally Moving on from the Relative Potency Approach to a Mixtures Approach for Polycyclic Aromatic Hydrocarbons (PAHs)
Authors: Haber Lynne T. , Pecquet Alison M. , Vincent Melissa J. , White Louise M.
Published: August 2022

For the past several decades, a relative potency approach has been used to estimate the human health risks from exposure to polycyclic aromatic hydrocarbon (PAH) mixtures. Risk estimates are derived using potency equivalence factors (PEFs; also called relative potency factors [RPFs]), based on the ratio of selected PAHs to benzo[a]pyrene (BaP), expressed qualitatively by orders of magnitude. To quantify PEFs for 18 selected carcinogenic PAHs, a systematic approach with a priori and dose response criteria was developed, building on draft work by the US EPA in 2010 and its review by US EPA Science Advisory Board (SAB) in 2011. An exhaustive search for carcinogenicity studies that included both target PAHs and BaP with environmentally relevant exposure routes found only 48 animal bioassay datasets (mostly pre-1992 based on skin painting). Only eight datasets provided adequate low-response data, and of these only four datasets were appropriate for modeling to estimate PEFs; only benzo[b]fluoranthene and cyclopenta[c,d]pyrene had a PEF that could be quantified. Thus, current knowledge of PAH carcinogenicity is insufficient to support quantitative PEFs for PAH mixtures. This highlights the long-acknowledged need for an interdisciplinary approach to estimate risks from PAH mixtures. Use of alternative and short-term toxicity testing methods, improved mixture characterization, understanding the fate and bioavailability of PAH mixtures, and understanding exposure route-related differences in carcinogenicity are discussed as ways to improve the understanding of the risks of PAHs.
A Free-Market Environmentalist Enquiry on Spain’s Energy Transition along with Its Recent Increasing Electricity Prices
Authors: Wang William Hongsong , Espinosa Victor I. , Huerta de Soto Jesús
Published: August 2022

This paper analyzes the Spanish energy transition’s general situation and its increasing electricity prices in recent years from a free-market environmentalist (FME) approach. We hypothesize and argue that high taxes, high government subsidies, and government industrial access restrictions breach private property rights, hindering Spain’s renewable energy (RE) development. Our paper discovers that Spain’s state-interventionist policies have increased the cost of the energy and power industries, leading to electricity prices remaining relatively high before and after the outbreak of the COVID-19 pandemic. After reviewing the literature on the FME approach and Spain’s case, a Box–Jenkins (ARIMA) model is used to clarify the economic performance of the Spanish electricity industry with a proposal for forecasting electricity prices. It is observed that Spain fails the EU and its national goal of providing an affordable energy price as a part of the green energy transition. Finally, free-market environmental solutions and policy reforms are proposed to facilitate Spain’s energy transition.
Therapists’ Emotional Responses in Individual Therapy with Depressed Adolescents: An Evaluation of the Data Structure of the Feeling-Word Checklist—28
Authors: Brøsholen Pernille , Ulberg Randi , Dahl Hanne-Sofie Johnsen , Thorén Agneta
Published: August 2022

Countertransference (CT) responses during therapy sessions can be understood as the therapist’s emotional reactions towards the patient. Within adolescents’ psychotherapy, little is known about the effects of the therapists’ feelings on treatment outcome. The Feeling-Word Checklist—28 (FWC-28) is a self-report questionnaire designed to evaluate the therapist’s in-session feelings during therapy with younger patients. The aim of the study was to evaluate the psychometric properties of the clinician-rated FWC-28 and explore the associations between the CT-subscales and therapeutic alliance. Data were collected from a randomized controlled trial in which 11 therapists specialized in child and adolescent psychotherapy treated 16- to 18-year-old patients (n = 62) with major depressive disorder in outpatient clinics. The patients received psychodynamic psychotherapy treatment over 28 sessions. Therapists rated their emotional responses towards their patients on FWC-28 after sessions 3, 12, 20, and 28. Principal component analysis (PCA) with oblique rotation was performed to find clinically meaningful subscales of the FWC-28. PCA revealed four clinically meaningful components termed as follows: inadequate, confident, motherly, and disengaged. The psychometric properties of the FWC and the reliability of the CT subscales measured with Cronbach’s alpha were acceptable. The therapist-reported alliance showed significant and clinically meaningful correlations with all CT-subscales. Our findings indicate that the checklist is adequate for clinical practice and countertransference research in adolescents’ psychotherapy.
The Aggregation and Dissolution of Citrate−Coated AgNPs in High Ammonia Nitrogen Wastewater and Sludge from UASB−Anammox Reactor
Authors: Jiang Jiachao , Wang Xin , Zhang Yuanyuan , Zhang Jiageng , Gu Xiujun , He Shilong , Duan Shuo , Ma Jianli , Wang Lizhang , Luo Ping
Published: August 2022

Silver nanoparticles (AgNPs) are released into the sewage pipes and ultimately wastewater treatment plants during manufacturing, use, and end–life disposal. AgNPs in wastewater treatment plants aggregate or dissolve, and may affect the microbial community and subsequent pollutant removal efficiency. This study aims to quantitatively investigate the fate of AgNPs in synthetic high ammonia nitrogen wastewater (SW) and sludge from an up–flow anaerobic sludge blanket (UASB) anammox reactor using a nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), transmission electron microscope (TEM), and atomic absorption spectroscopy (AAS). Results showed that 18.1 mM NH4+, 2.11 mM Mg2+ in SW caused less negative zeta potential (ζ−potential, −18.4 vs. −37.4 mV), aggregation (388.8 vs. 21.5 nm), and settlement (80%) of citrate−coated AgNPs (cit−AgNPs) in 220 min. The presence of 18.5 mM Cl− in SW formed AgCl2−, AgCl(aq) and eventually promoted the dissolution (9.3%) of cit−AgNPs. Further exposure of SW−diluted AgNPs to sludge (42 mg L−1 humic acid) and induced a more negative ζ−potential (−22.2 vs. −18.4 mV) and smaller aggregates (313.4 vs. 388.8 nm) due to the steric and hindrance effect. The promoted Ag dissolution (34.4% vs. 9.3%) was also observed after the addition of sludge and the possible reason may be the production of Ag(NH3)2+ by the coexistence of HA from sludge and NH4+ from SW. These findings on the fate of AgNPs can be used to explain why AgNPs had limited effects on the sludge−retained bacteria which are responsible for the anammox process.
Measure of Utilizing Space Database Information for Improvement of Efficient Disaster Management (Focusing on Nuclear Power Plant Accidents)
Authors: Lee Bomi , Kang Aetti , Ham Sungil
Published: August 2022

The damage caused by disasters is increasing worldwide, with hundreds of thousands of deaths due to the occurrence of complex large-scale disasters such as the 2010 Haiti earthquake and the 2004 Indian tsunami. South Korea has also experienced human casualties and damage to property caused by large-scale disasters in the past 10 years. Accordingly, a disaster-appropriate response measure is needed. Thus, we conducted this study to present a measure of utilizing spatial database and image information to improve the efficiency of disaster management that is operated based on the country’s existing national disaster management system. We present an efficient disaster response measure that differs from the existing collection-, reporting-, and propagation-oriented operating methods of disaster information through the use of spatial database and image-based information that can be combined with mandatory information with regard to nuclear power plant accidents. Thus, this study contributes to deriving a system that could collect and provide information rapidly at the time of disaster by defining the attribute and spatial information required at the time of disaster during nuclear power plant accidents and by deriving available systems and providing institutions.
Phospholipase A1 Member A Deficiency Alleviates Mannan-Induced Psoriatic Arthritis in Mice Model
Authors: Zhao Yang , Aoudjit Fawzi , Bourgoin Sylvain G.
Published: August 2022

Synovial fluids from rheumatoid and psoriatic arthritis patients have high levels of PLA1A. The current study was to understand PLA1A functions in the pathophysiology of rheumatic diseases. We generated Pla1a−/− mice to assess their phenotype and the impact of PLA1A deficiency on the development of mannan-induced psoriatic arthritis (MIP). Mice were evaluated routinely for the induced symptoms. On the day of sacrifice, blood samples were collected for hematology analysis and prepared for plasma. Livers were collected. Lymph node immune cells were analyzed using flow cytometry. We performed μCT scans of hind paws from naïve and mannan-induced female mice. Cytokines/chemokines were quantified using Luminex in hind paw tissues and plasma of female mice. Pla1a−/− mice showed a slight increase in circulating and lymph node lymphocytes. CD4+ T cells contributed most to this increase in lymph nodes (p = 0.023). In the MIP model, the lymph node ratios of CD3+ to CD19+ and CD4+ to CD8+ were higher in Pla1a−/− mice. Pla1a−/− mice were less susceptible to MIP (p < 0.001) and showed reduced bone erosions. Pla1a−/− mice also showed reduced IL-17, KC, IP-10, MIP-1β, LIF, and VEGF in hind paw tissues as compared to WT mice (p < 0.05). These findings indicated that PLA1A deficiency protected from the development of the MIP disease. The data suggested that PLA1A could contribute to MIP through increased activation of lymphocytes, possibly those producing IL-17.
Utilization of Aminoguanidine Prevents Cytotoxic Effects of Semen
Authors: Harms Mirja , von Maltitz Pascal , Groß Rüdiger , Mayer Benjamin , Deniz Miriam , Müller Janis , Münch Jan
Published: August 2022

Studies of human semen in cell or tissue culture are hampered by the high cytotoxic activity of this body fluid. The components responsible for the cell damaging activity of semen are amine oxidases, which convert abundant polyamines, such as spermine or spermidine in seminal plasma into toxic intermediates. Amine oxidases are naturally present at low concentrations in seminal plasma and at high concentrations in fetal calf serum, a commonly used cell culture supplement. Here, we show that, in the presence of fetal calf serum, seminal plasma, as well as the polyamines spermine and spermidine, are highly cytotoxic to immortalized cells, primary blood mononuclear cells, and vaginal tissue. Thus, experiments investigating the effect of polyamines and seminal plasma on cellular functions should be performed with great caution, considering the confounding cytotoxic effects. The addition of the amine oxidase inhibitor aminoguanidine to fetal calf serum and/or the utilization of serum-free medium greatly reduced this serum-induced cytotoxicity of polyamines and seminal plasma in cell lines, primary cells, and tissues and, thus, should be implemented in all future studies analyzing the role of polyamines and semen on cellular functions.
Helicobacter pylori, Protected from Antibiotics and Stresses Inside Candida albicans Vacuoles, Cause Gastritis in Mice
Authors: Hiengrach Pratsanee , Panpetch Wimonrat , Chindamporn Ariya , Leelahavanichkul Asada
Published: August 2022

Due to (i) the simultaneous presence of Helicobacter pylori (ulcer-induced bacteria) and Candida albicans in the stomach and (ii) the possibility of prokaryotic–eukaryotic endosymbiosis (intravacuolar H. pylori in the yeast cells) under stresses, we tested this symbiosis in vitro and in vivo. To that end, intravacuolar H. pylori were induced by the co-incubation of C. albicans with H. pylori under several stresses (acidic pH, non-H. pylori-enrichment media, and aerobic environments); the results were detectable by direct microscopy (wet mount) and real-time polymerase chain reaction (PCR). Indeed, intravacuolar H. pylori were predominant under all stresses, especially the lower pH level (pH 2–3). Interestingly, the H. pylori (an amoxicillin-sensitive strain) inside C. albicans were protected from the antibiotic (amoxicillin), while extracellular H. pylori were neutralizable, as indicated by the culture. In parallel, the oral administration of intravacuolar H. pylori in mice caused H. pylori colonization in the stomach resulting in gastritis, as indicated by gastric histopathology and tissue cytokines, similar to the administration of free H. pylori (extra-Candida bacteria). In conclusion, Candida protected H. pylori from stresses and antibiotics, and the intravacuolar H. pylori were able to be released from the yeast cells, causing gastric inflammation with neutrophil accumulations.
The Role of Neuro-Immune Interaction in Chronic Pain Conditions; Functional Somatic Syndrome, Neurogenic Inflammation, and Peripheral Neuropathy
Authors: Meade Elaine , Garvey Mary
Published: August 2022

Functional somatic syndromes are increasingly diagnosed in chronically ill patients presenting with an array of symptoms not attributed to physical ailments. Conditions such as chronic fatigue syndrome, fibromyalgia syndrome, or irritable bowel syndrome are common disorders that belong in this broad category. Such syndromes are characterised by the presence of one or multiple chronic symptoms including widespread musculoskeletal pain, fatigue, sleep disorders, and abdominal pain, amongst other issues. Symptoms are believed to relate to a complex interaction of biological and psychosocial factors, where a definite aetiology has not been established. Theories suggest causative pathways between the immune and nervous systems of affected individuals with several risk factors identified in patients presenting with one or more functional syndromes. Risk factors including stress and childhood trauma are now recognised as important contributors to chronic pain conditions. Emotional, physical, and sexual abuse during childhood is considered a severe stressor having a high prevalence in functional somatic syndrome suffers. Such trauma permanently alters the biological stress response of the suffers leading to neuroexcitatory and other nerve issues associated with chronic pain in adults. Traumatic and chronic stress results in epigenetic changes in stress response genes, which ultimately leads to dysregulation of the hypothalamic-pituitary axis, the autonomic nervous system, and the immune system manifesting in a broad array of symptoms. Importantly, these systems are known to be dysregulated in patients suffering from functional somatic syndrome. Functional somatic syndromes are also highly prevalent co-morbidities of psychiatric conditions, mood disorders, and anxiety. Consequently, this review aims to provide insight into the role of the nervous system and immune system in chronic pain disorders associated with the musculoskeletal system, and central and peripheral nervous systems.
Virus-like Particles: Fundamentals and Biomedical Applications
Authors: Mejía-Méndez Jorge L. , Vazquez-Duhalt Rafael , Hernández Luis R. , Sánchez-Arreola Eugenio , Bach Horacio
Published: August 2022

Nanotechnology is a fast-evolving field focused on fabricating nanoscale objects for industrial, cosmetic, and therapeutic applications. Virus-like particles (VLPs) are self-assembled nanoparticles whose intrinsic properties, such as heterogeneity, and highly ordered structural organization are exploited to prepare vaccines; imaging agents; construct nanobioreactors; cancer treatment approaches; or deliver drugs, genes, and enzymes. However, depending upon the intrinsic features of the native virus from which they are produced, the therapeutic performance of VLPs can vary. This review compiles the recent scientific literature about the fundamentals of VLPs with biomedical applications. We consulted different databases to present a general scenario about viruses and how VLPs are produced in eukaryotic and prokaryotic cell lines to entrap therapeutic cargo. Moreover, the structural classification, morphology, and methods to functionalize the surface of VLPs are discussed. Finally, different characterization techniques required to examine the size, charge, aggregation, and composition of VLPs are described.
Transgenic Mice for the Translational Study of Neuropathic Pain and Dystonia
Authors: Scuteri Damiana , Hamamura Kengo , Watanabe Chizuko , Tonin Paolo , Bagetta Giacinto , Corasaniti Maria Tiziana
Published: August 2022

Murine models are fundamental in the study of clinical conditions and the development of new drugs and treatments. Transgenic technology has started to offer advantages in oncology, encompassing all research fields related to the study of painful syndromes. Knockout mice or mice overexpressing genes encoding for proteins linked to pain development and maintenance can be produced and pain models can be applied to transgenic mice to model the most disabling neurological conditions. Due to the association of movement disorders with sensitivity and pain processing, our group focused for the first time on the role of the torsinA gene GAG deletion—responsible for DYT1 dystonia—in baseline sensitivity and neuropathic responses. The aim of the present report are to review the complex network that exists between the chaperonine-like protein torsinA and the baseline sensitivity pattern—which are fundamental in neuropathic pain—and to point at its possible role in neurodegenerative diseases.
Consequences of Acute or Chronic Methylphenidate Exposure Using Ex Vivo Neurochemistry and In Vivo Electrophysiology in the Prefrontal Cortex and Striatum of Rats
Authors: Di Miceli Mathieu , Derf Asma , Gronier Benjamin
Published: August 2022

Methylphenidate (MPH) is among the main drugs prescribed to treat patients with attention-deficit and hyperactivity disease (ADHD). MPH blocks both the norepinephrine and dopamine reuptake transporters (NET and DAT, respectively). Our study was aimed at further understanding the mechanisms by which MPH could modulate neurotransmitter efflux, using ex vivo radiolabelled neurotransmitter assays isolated from rats. Here, we observed significant dopamine and norepinephrine efflux from the prefrontal cortex (PFC) after MPH (100 µM) exposure. Efflux was mediated by both dopamine and norepinephrine terminals. In the striatum, MPH (100 µM) triggered dopamine efflux through both sodium- and vesicular-dependent mechanisms. Chronic MPH exposure (4 mg/kg/day/animal, voluntary oral intake) for 15 days, followed by a 28-day washout period, increased the firing rate of PFC pyramidal neurons, assessed by in vivo extracellular single-cell electrophysiological recordings, without altering the responses to locally applied NMDA, via micro-iontophoresis. Furthermore, chronic MPH treatment resulted in decreased efficiency of extracellular dopamine to modulate NMDA-induced firing activities of medium spiny neurons in the striatum, together with lower MPH-induced (100 µM) dopamine outflow, suggesting desensitization to both dopamine and MPH in striatal regions. These results indicate that MPH can modulate neurotransmitter efflux in brain regions enriched with dopamine and/or norepinephrine terminals. Further, long-lasting alterations of striatal and prefrontal neurotransmission were observed, even after extensive washout periods. Further studies will be needed to understand the clinical implications of these findings.
Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies
Authors: Salg Gabriel Alexander , Blaeser Andreas , Gerhardus Jamina Sofie , Hackert Thilo , Kenngott Hannes Goetz
Published: August 2022

Among advanced therapy medicinal products, tissue-engineered products have the potential to address the current critical shortage of donor organs and provide future alternative options in organ replacement therapy. The clinically available tissue-engineered products comprise bradytrophic tissue such as skin, cornea, and cartilage. A sufficient macro- and microvascular network to support the viability and function of effector cells has been identified as one of the main challenges in developing bioartificial parenchymal tissue. Three-dimensional bioprinting is an emerging technology that might overcome this challenge by precise spatial bioink deposition for the generation of a predefined architecture. Bioinks are printing substrates that may contain cells, matrix compounds, and signaling molecules within support materials such as hydrogels. Bioinks can provide cues to promote vascularization, including proangiogenic signaling molecules and cocultured cells. Both of these strategies are reported to enhance vascularization. We review pre-, intra-, and postprinting strategies such as bioink composition, bioprinting platforms, and material deposition strategies for building vascularized tissue. In addition, bioconvergence approaches such as computer simulation and artificial intelligence can support current experimental designs. Imaging-derived vascular trees can serve as blueprints. While acknowledging that a lack of structured evidence inhibits further meta-analysis, this review discusses an end-to-end process for the fabrication of vascularized, parenchymal tissue.
T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer
Authors: Mazzotti Lucia , Gaimari Anna , Bravaccini Sara , Maltoni Roberta , Cerchione Claudio , Juan Manel , Navarro Europa Azucena-Gonzalez , Pasetto Anna , Nascimento Silva Daniela , Ancarani Valentina , Sambri Vittorio , Calabrò Luana , Martinelli Giovanni , Mazza Massimiliano
Published: August 2022

The immune system is a dynamic feature of each individual and a footprint of our unique internal and external exposures. Indeed, the type and level of exposure to physical and biological agents shape the development and behavior of this complex and diffuse system. Many pathological conditions depend on how our immune system responds or does not respond to a pathogen or a disease or on how the regulation of immunity is altered by the disease itself. T-cells are important players in adaptive immunity and, together with B-cells, define specificity and monitor the internal and external signals that our organism perceives through its specific receptors, TCRs and BCRs, respectively. Today, high-throughput sequencing (HTS) applied to the TCR repertoire has opened a window of opportunity to disclose T-cell repertoire development and behavior down to the clonal level. Although TCR repertoire sequencing is easily accessible today, it is important to deeply understand the available technologies for choosing the best fit for the specific experimental needs and questions. Here, we provide an updated overview of TCR repertoire sequencing strategies, providers and applications to infectious diseases and cancer to guide researchers’ choice through the multitude of available options. The possibility of extending the TCR repertoire to HLA characterization will be of pivotal importance in the near future to understand how specific HLA genes shape T-cell responses in different pathological contexts and will add a level of comprehension that was unthinkable just a few years ago.
The Conservation of Long Intergenic Non-Coding RNAs and Their Response to Verticillium dahliae Infection in Cotton
Authors: Chen Li , Shen Enhui , Zhao Yunlei , Wang Hongmei , Wilson Iain , Zhu Qian-Hao
Published: August 2022

Long intergenic non-coding RNAs (lincRNAs) have been demonstrated to be vital regulators of diverse biological processes in both animals and plants. While many lincRNAs have been identified in cotton, we still know little about the repositories and conservativeness of lincRNAs in different cotton species or about their role in responding to biotic stresses. Here, by using publicly available RNA-seq datasets from diverse sources, including experiments of Verticillium dahliae (Vd) infection, we identified 24,425 and 17,713 lincRNAs, respectively, in Gossypium hirsutum (Ghr) and G. barbadense (Gba), the two cultivated allotetraploid cotton species, and 6933 and 5911 lincRNAs, respectively, in G. arboreum (Gar) and G. raimondii (Gra), the two extant diploid progenitors of the allotetraploid cotton. While closely related subgenomes, such as Ghr_At and Gba_At, tend to have more conserved lincRNAs, most lincRNAs are species-specific. The majority of the synthetic and transcribed lincRNAs (78.2%) have a one-to-one orthologous relationship between different (sub)genomes, although a few of them (0.7%) are retained in all (sub)genomes of the four species. The Vd responsiveness of lincRNAs seems to be positively associated with their conservation level. The major functionalities of the Vd-responsive lincRNAs seem to be largely conserved amongst Gra, Ghr, and Gba. Many Vd-responsive Ghr-lincRNAs overlap with Vd-responsive QTL, and several lincRNAs were predicted to be endogenous target mimicries of miR482/2118, with a pair being highly conserved between Ghr and Gba. On top of the confirmation of the feature characteristics of the lincRNAs previously reported in cotton and other species, our study provided new insights into the conservativeness and divergence of lincRNAs during cotton evolution and into the relationship between the conservativeness and Vd responsiveness of lincRNAs. The study also identified candidate lincRNAs with a potential role in disease response for functional characterization.
Unique Features of River Lamprey (Lampetra fluviatilis) Myogenesis
Authors: Migocka-Patrzałek Marta , Kujawa Roman , Podlasz Piotr , Juchno Dorota , Haczkiewicz-Leśniak Katarzyna , Daczewska Małgorzata
Published: August 2022

The river lamprey (L. fluviatilis) is a representative of the ancestral jawless vertebrate group. We performed a histological analysis of trunk muscle fiber differentiation during embryonal, larval, and adult musculature development in this previously unstudied species. Investigation using light, transmission electron (TEM), and confocal microscopy revealed that embryonal and larval musculature differs from adult muscle mass. Here, we present the morphological analysis of L. fluviatilis myogenesis, from unsegmented mesoderm through somite formation, and their differentiation into multinucleated muscle lamellae. Our analysis also revealed the presence of myogenic factors LfPax3/7 and Myf5 in the dermomyotome. In the next stages of development, two types of muscle lamellae can be distinguished: central surrounded by parietal. This pattern is maintained until adulthood, when parietal muscle fibers surround the central muscles on both sides. The two types show different morphological characteristics. Although lampreys are phylogenetically distant from jawed vertebrates, somite morphology, especially dermomyotome function, shows similarity. Here we demonstrate that somitogenesis is a conservative process among all vertebrates. We conclude that river lamprey myogenesis shares features with both ancestral and higher vertebrates.
Hypercholesterolemia-Induced HDL Dysfunction Can Be Reversed: The Impact of Diet and Statin Treatment in a Preclinical Animal Model
Authors: Schoch Leonie , Sutelman Pablo , Suades Rosa , Casani Laura , Padro Teresa , Badimon Lina , Vilahur Gemma
Published: August 2022

High-density lipoproteins (HDL) undergo adverse remodeling and loss of function in the presence of comorbidities. We assessed the potential of lipid-lowering approaches (diet and rosuvastatin) to rescue hypercholesterolemia-induced HDL dysfunction. Hypercholesterolemia was induced in 32 pigs for 10 days. Then, they randomly received one of the 30-day interventions: (I) hypercholesterolemic (HC) diet; (II) HC diet + rosuvastatin; (III) normocholesterolemic (NC) diet; (IV) NC diet + rosuvastatin. We determined cholesterol efflux capacity (CEC), antioxidant potential, HDL particle number, HDL apolipoprotein content, LDL oxidation, and lipid levels. Hypercholesterolemia time-dependently impaired HDL function (−62% CEC, −11% antioxidant index (AOI); p < 0.01), increased HDL particles numbers 2.8-fold (p < 0.0001), reduced HDL-bound APOM (−23%; p < 0.0001), and increased LDL oxidation 1.7-fold (p < 0.0001). These parameters remained unchanged in animals on HC diet alone up to day 40, while AOI deteriorated up to day 25 (−30%). The switch to NC diet reversed HDL dysfunction, restored apolipoprotein M content and particle numbers, and normalized cholesterol levels at day 40. Rosuvastatin improved HDL, AOI, and apolipoprotein M content. Apolipoprotein A-I and apolipoprotein C-III remained unchanged. Lowering LDL-C levels with a low-fat diet rescues HDL CEC and antioxidant potential, while the addition of rosuvastatin enhances HDL antioxidant capacity in a pig model of hypercholesterolemia. Both strategies restore HDL-bound apolipoprotein M content.
Biomarkers for the Diagnosis of Alzheimer’s Disease in Clinical Practice: The Role of CSF Biomarkers during the Evolution of Diagnostic Criteria
Authors: Dulewicz Maciej , Kulczyńska-Przybik Agnieszka , Mroczko Piotr , Kornhuber Johannes , Lewczuk Piotr , Mroczko Barbara
Published: August 2022

Alzheimer’s disease (AD) is a progressive condition and the most common cause of dementia worldwide. The neuropathological changes characteristic of the disorder can be successfully detected before the development of full-blown AD. Early diagnosis of the disease constitutes a formidable challenge for clinicians. CSF biomarkers are the in vivo evidence of neuropathological changes developing in the brain of dementia patients. Therefore, measurement of their concentrations allows for improved accuracy of clinical diagnosis. Moreover, AD biomarkers may provide an indication of disease stage. Importantly, the CSF biomarkers of AD play a pivotal role in the new diagnostic criteria for the disease, and in the recent biological definition of AD by the National Institute on Aging, NIH and Alzheimer’s Association. Due to the necessity of collecting CSF by lumbar puncture, the procedure seems to be an important issue not only from a medical, but also a legal, viewpoint. Furthermore, recent technological advances may contribute to the automation of AD biomarkers measurement and may result in the establishment of unified cut-off values and reference limits. Moreover, a group of international experts in the field of AD biomarkers have developed a consensus and guidelines on the interpretation of CSF biomarkers in the context of AD diagnosis. Thus, technological advancement and expert recommendations may contribute to a more widespread use of these diagnostic tests in clinical practice to support a diagnosis of mild cognitive impairment (MCI) or dementia due to AD. This review article presents up-to-date data regarding the usefulness of CSF biomarkers in routine clinical practice and in biomarkers research.
Beyond Cancer: Regulation and Function of PD-L1 in Health and Immune-Related Diseases
Authors: Beenen Amke C. , Sauerer Tatjana , Schaft Niels , Dörrie Jan
Published: August 2022

Programmed Cell Death 1 Ligand 1 (PD-L1, CD274, B7-H1) is a transmembrane protein which is strongly involved in immune modulation, serving as checkpoint regulator. Interaction with its receptor, Programmed Cell Death Protein 1 (PD-1), induces an immune-suppressive signal, which modulates the activity of T cells and other effector cells. This mediates peripheral tolerance and contributes to tumor immune escape. PD-L1 became famous due to its deployment in cancer therapy, where blockage of PD-L1 with the help of therapeutic antagonistic antibodies achieved impressive clinical responses by reactivating effector cell functions against tumor cells. Therefore, in the past, the focus has been placed on PD-L1 expression and its function in various malignant cells, whereas its role in healthy tissue and diseases apart from cancer remained largely neglected. In this review, we summarize the function of PD-L1 in non-cancerous cells, outlining its discovery and origin, as well as its involvement in different cellular and immune-related processes. We provide an overview of transcriptional and translational regulation, and expression patterns of PD-L1 in different cells and organs, and illuminate the involvement of PD-L1 in different autoimmune diseases as well as in the context of transplantation and pregnancy.
Using Drones for Thermal Imaging Photography and Building 3D Images to Analyze the Defects of Solar Modules
Authors: Liao Kuo-Chien , Wu Hom-Yu , Wen Hung-Ta
Published: August 2022

In this research, drones were used to capture thermal images and detect different types of failure of solar modules, and MATLAB® image analysis was also conducted to evaluate the health of the solar modules. The processes included image acquisition and transmission by drone, grayscale conversion, filtering, 3D image construction, and analysis. The analyzed targets were the solar modules installed on buildings. The results showed that the employment of drones to monitor solar module farms could significantly improve inspection efficiency. Moreover, by combining the mean and median filtering techniques, an innovative box filtering method was successfully created. Additionally, this study compared the differences between the mean, median, and box filtering techniques, and proved that the 3D image improved by box filtering is a more convenient and accurate way to check the health of solar modules than the mean and median filtering methods. In addition, this new method can simplify the maintenance process, as it helps maintenance personnel to determine whether to replace the solar modules on site, achieving the goal of power generation efficiency enhancement. It is worth noting that 3D image recognition technology can enhance the clarity of thermal images, thereby providing maintenance personnel with better defect diagnosis capability. It is also able to provide the temperature value of the defect zone, and to indicate the scale of defects through the cumulative temperature chart, so the 3D image is qualified as a quantitative and qualitative indicator. The analysis of the transmitted image is innovative that it not only can locate the defect area of the module, but also can display the temperature of the module, providing more information for maintenance personnel.
Prognostic Value of Catestatin in Severe COVID-19: An ICU-Based Study
Authors: Kljakovic-Gaspic Toni , Tokic Daria , Martinovic Dinko , Kumric Marko , Supe-Domic Daniela , Stojanovic Stipic Sanda , Delic Nikola , Vrdoljak Josip , Vilovic Marino , Ticinovic Kurir Tina , Bozic Josko
Published: August 2022

Catestatin is a pleiotropic peptide with a wide range of immunomodulatory effects. Considering that patients with a severe COVID-19 infection have a major immunological dysregulation, the aim of this study was to evaluate catestatin levels in patients with COVID-19 treated in the intensive care unit (ICU) and to compare them between the fatal and non-fatal outcomes. The study included 152 patients with severe COVID-19, out of which 105 had a non-fatal outcome and 47 had a fatal outcome. Serum catestatin levels were estimated by an enzyme-linked immunosorbent assay in a commercially available diagnostic kit. The results show that catestatin levels were significantly lower in the fatal group compared to the non-fatal group (16.6 ± 7.8 vs. 23.2 ± 9.2 ng/mL; p < 0.001). Furthermore, there was a significant positive correlation between serum catestatin levels and vitamin D levels (r = 0.338; p < 0.001) while there was also a significant positive correlation between serum catestatin levels and growth differentiation factor-15 (GDF-15) levels (r = −0.345; p < 0.001). Furthermore, multivariate logistic regression showed that catestatin, GDF-15 and leukocyte count were significant predictors for COVID-19 survival. These findings imply that catestatin could be playing a major immunomodulatory role in the complex pathophysiology of the COVID-19 infection and that serum catestatin could also be a predictor of a poor COVID-19 outcome.
The Use of Non-Invasive Continuous Blood Pressure Measuring (ClearSight®) during Central Neuraxial Anaesthesia for Caesarean Section—A Retrospective Validation Study
Authors: Helmer Philipp , Helf Daniel , Sammeth Michael , Winkler Bernd , Hottenrott Sebastian , Meybohm Patrick , Kranke Peter
Published: August 2022

The close monitoring of blood pressure during a caesarean section performed under central neuraxial anaesthesia should be the standard of safe anaesthesia. As classical oscillometric and invasive blood pressure measuring have intrinsic disadvantages, we investigated a novel, non-invasive technique for continuous blood pressure measuring. Methods: In this monocentric, retrospective data analysis, the reliability of continuous non-invasive blood pressure measuring using ClearSight® (Edwards Lifesciences Corporation) is validated in 31 women undergoing central neuraxial anaesthesia for caesarean section. In addition, patients and professionals evaluated ClearSight® through questioning. Results: 139 measurements from 11 patients were included in the final analysis. Employing Bland–Altman analyses, we identified a bias of −10.8 mmHg for systolic, of −0.45 mmHg for diastolic and of +0.68 mmHg for mean arterial blood pressure measurements. Pooling all paired measurements resulted in a Pearson correlation coefficient of 0.7 for systolic, of 0.67 for diastolic and of 0.75 for mean arterial blood pressure. Compensating the interindividual differences in linear regressions of the paired measurements provided improved correlation coefficients of 0.73 for systolic, of 0.9 for diastolic and of 0.89 for mean arterial blood pressure measurements. Discussion: Diastolic and mean arterial blood pressure are within an acceptable range of deviation from the reference method, according to the Association for the Advancement of Medical Instrumentation (AAMI) in the patient collective under study. Both patients and professionals prefer ClearSight® to oscillometric blood pressure measurement in regard of comfort and handling.
Periacetabular Tumour Resection under Anterosuperior Iliac Spine Allows Better Alloprosthetic Reconstruction than Above: Bone Contact Matters
Authors: Bruschi Alessandro , Cevolani Luca , Spazzoli Benedetta , Focaccia Marco , Pasini Stefano , Frisoni Tommaso , Donati Davide Maria
Published: August 2022

Background: Periacetabular resections are more affected by late complications than other pelvic resections. Reconstruction using bone allograft is considered a suitable solution. However, it is still not clear how the bone-allograft contact surface impacts on mechanical and functional outcome. Materials and methods: This paper presents the results of a retrospective analysis of 33 patients with resection of the entire acetabulum and reconstruction with an allograft-prosthetic composite for the period 1999 to 2010. Patients were divided in two groups, based on type of resection. In Group 1. patients had resections under anterosuperior iliac spine allowing the highest bone-allograft surface contact in reconstruction, while in Group 2 patients had resections over it. Results: Mechanical survival of the implant and Musculoskeletal Tumor Society functional score were calculated. Impact of age and artificial ligament were investigated as well. Patients in Group 1 had 38% mechanical failure rate of the implant while patients in Group 2 had 88%. Average functional score was higher in Group 1 compared with patients in Group 2. An artificial ligament was shown to have non-significant impact on survival of the reconstruction in Group 1, while significantly improving survival of reconstruction in Group 2. Conclusion: Bone-allograft contact matters: resection under anterosuperior iliac spine allows better mechanical survival and offers better reconstruction functional scores.