bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical outcome of microscopic lumbar spinous process–splitting laminectomy
Authors: Nomura Hiroshi , Yanagisawa Yoshikazu , Arima Junichi , Oga Masayoshi
Published: May 2014

Object

The authors sought to quantify the clinical outcome of microscopic lumbar spinous process–splitting laminectomy in patients with lumbar spinal stenosis (LSS). They performed a microscopic laminectomy in which the spinous process is split longitudinally into 2 halves. For single-level decompression, they inserted a tubular retractor between the split process.

Methods

Data obtained in a total of 124 patients were retrospectively analyzed. Patients were divided into 2 groups: the “slip” group, comprising patients with spondylolisthesis-type LSS with vertebral body slippage (Group 1), and the “nonslip” group, comprising patients with spondylosis-type LSS without vertebral body slippage or with LSS due to central protrusion of lumbar disc herniation. Clinical outcome in all patients was evaluated by using the Japanese Orthopaedic Association score. In the slip group, slippage and instability rates were evaluated by using pre- and postoperative dynamic radiographs in the sagittal plane. Postoperative CT images were used to evaluate bony union at 2 sites: a region between the left and right portions of the halved spinous process and a region between the base of the halved process and vertebral arch. Signal intensity of the multifidus muscle at individual decompression levels was evaluated on pre- and postoperative T1- and T2-weighted MR images.

Results

Preoperative clinical symptoms improved significantly after surgery in all patients. Slippage and instability rates in the slip group showed no significant differences when pre- and postoperative conditions were compared. Union rates at the region between the left and right portions of the halved process and the region between the base of the halved process and vertebral arch were 97.1% and 82.5%, respectively. Magnetic resonance imaging showed only a small amount of fat infiltration in the multifidus muscle after surgery in 12.2% of cases.

Conclusions

The authors recommend microscopic lumbar spinous process–splitting laminectomy as a promising minimally invasive surgery for the treatment of LSS.
Patterns of Precipitation and Convection Occurrence over the Mediterranean Basin Derived from a Decade of Microwave Satellite Observations
Authors: Alhammoud Bahjat , Claud Chantal , Funatsu Beatriz , Béranger Karine , Chaboureau Jean-Pierre
Published: May 2014

The Mediterranean region is characterized by its vulnerability to changes in the water cycle, with the impact of global warming on the water resources being one of the major concerns in social, economical and scientific ambits. Even if precipitation is the best-known term of the Mediterranean water budget, large uncertainties remain due to the lack of suitable offshore observational data. In this study, we use the data provided by the Advanced Microwave Sounding Unit-B (AMSU-B) on board NOAA satellites to detect and analyze precipitating and convective events over the last decade at spatial resolution of 0.2° latitude × 0.2° longitude. AMSU-B observation shows that rain occurrence is widespread over the Mediterranean in wintertime while reduced in the eastern part of the basin in summer. Both precipitation and convection occurrences display a weak diurnal cycle over sea. In addition, convection occurrences, which are essentially located over land during summertime, shift to mostly over the sea during autumn with maxima in the Ionian sub-basin and the Adriatic Sea. Precipitation occurrence is also inferred over the sea from two other widely used climatological datasets, HOAPS (Hamburg Ocean Atmosphere Parameters and Fluxes from Satellite Data) and the European Centre for Medium-Range Weather Forecasts (ECMWF) reanalysis interim (ERA-Interim). There is generally a rather fair agreement between these climatologies for describing the large-scale patterns such as the strong latitudinal gradient of rain and eastward rain signal propagation. Furthermore, the higher spatial resolution of AMSU-B measurements (16 km at nadir) gives access to mesoscale details in the region (e.g., coastal areas). AMSU-B measurements show less rain occurrences than HOAPS during wintertime, thereby suggesting that some of the thresholds used in our method might be too stringent during this season. We also observed that convection occurrences in ERA-Interim are systematically lower than those derived from AMSU-B. These results are potentially valuable to evaluate the rainfall parameterization in weather and climate models and to constrain ocean models.
Seasonal and Diurnal Variations of Total Gaseous Mercury in Urban Houston, TX, USA
Authors: Lan Xin , Talbot Robert , Laine Patrick , Lefer Barry , Flynn James , Torres Azucena
Published: May 2014

Total gaseous mercury (THg) observations in urban Houston, over the period from August 2011 to October 2012, were analyzed for their seasonal and diurnal characteristics. Our continuous measurements found that the median level of THg was 172 parts per quadrillion by volume (ppqv), consistent with the current global background level. The seasonal variation showed that the highest median THg mixing ratios occurred in summer and the lowest ones in winter. This seasonal pattern was closely related to the frequency of THg episodes, energy production/consumption and precipitation in the area. The diurnal variations of THg exhibited a pattern where THg accumulated overnight and reached its maximum level right before sunrise, followed by a rapid decrease after sunrise. This pattern was clearly influenced by planetary boundary layer (PBL) height and horizontal winds, including the complex sea breeze system in the Houston area. A predominant feature of THg in the Houston area was the frequent occurrence of large THg spikes. Highly concentrated pollution plumes revealed that mixing ratios of THg were related to not only the combustion tracers CO, CO2, and NO, but also CH4 which is presumably released from oil and natural gas operations, landfills and waste treatment. Many THg episodes occurred simultaneously with peaks in CO, CO2, CH4, NOx, and/or SO2, suggesting possible contributions from similar sources with multi-source types. Our measurements revealed that the mixing ratios and variability of THg were primarily controlled by nearby mercury sources.
Advanced Cell Culture Techniques for Cancer Drug Discovery
Authors: Lovitt Carrie , Shelper Todd , Avery Vicky
Published: May 2014

Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment. Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely characterizes the disease state have been undertaken by a number of laboratories around the world. These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor cells. In addition, a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two-dimensional monolayer conditions do not respond to cancer therapeutics/compounds in a similar manner. Recent studies have demonstrated the potential of utilizing 3D cell culture models in drug discovery programs; however, it is evident that further research is required for the development of more complex models that incorporate the majority of the cellular and physical properties of a tumor.
Embroidery and Related Manufacturing Techniques for Wearable Antennas: Challenges and Opportunities
Authors: Tsolis Aris , Whittow William , Alexandridis Antonis , Vardaxoglou J.
Published: May 2014

This paper will review the evolution of wearable textile antennas over the last couple of decades. Particular emphasis will be given to the process of embroidery. This technique is advantageous for the following reasons: (i) bespoke or mass produced designs can be manufactured using digitized embroidery machines; (ii) glue is not required and (iii) the designs are aesthetic and are integrated into clothing rather than being attached to it. The embroidery technique will be compared to alternative manufacturing processes. The challenges facing the industrial and public acceptance of this technology will be assessed. Hence, the key opportunities will be highlighted.
The Real-Time Optimisation of DNO Owned Storage Devices on the LV Network for Peak Reduction
Authors: Rowe Matthew , Yunusov Timur , Haben Stephen , Holderbaum William , Potter Ben
Published: May 2014

Energy storage is a potential alternative to conventional network reinforcement of the low voltage (LV) distribution network to ensure the grid’s infrastructure remains within its operating constraints. This paper presents a study on the control of such storage devices, owned by distribution network operators. A deterministic model predictive control (MPC) controller and a stochastic receding horizon controller (SRHC) are presented, where the objective is to achieve the greatest peak reduction in demand, for a given storage device specification, taking into account the high level of uncertainty in the prediction of LV demand. The algorithms presented in this paper are compared to a standard set-point controller and bench marked against a control algorithm with a perfect forecast. A specific case study, using storage on the LV network, is presented, and the results of each algorithm are compared. A comprehensive analysis is then carried out simulating a large number of LV networks of varying numbers of households. The results show that the performance of each algorithm is dependent on the number of aggregated households. However, on a typical aggregation, the novel SRHC algorithm presented in this paper is shown to outperform each of the comparable storage control techniques.
Lifestyle Practices and Obesity in Malaysian Adolescents
Authors: Teo Pey , Nurul-Fadhilah Abdullah , Aziz Mohd , Hills Andrew , Foo Leng
Published: May 2014

Aim: To determine the influence of physical activity (PA) and sedentary behavior (SB) on obesity profiles of 454 Malaysian adolescents aged 12 to 19. Methods: Validated PA and SB questionnaires were used and body composition assessed using anthropometry and dual-energy X-ray absorptiometry (DXA). Results: Gender-specific multivariate analyses showed boys with high levels of total PA and moderate-to-vigorous physical activity (MVPA) exhibited significantly lower levels of total body fat, percent body fat and android fat mass compared with low PA and MVPA groups, after adjusting for potential confounders. Girls with high SB levels showed significantly higher BMI, waist circumference and DXA-derived body fat indices than those at lower SB level. Multiple logistic analyses indicated that boys with low levels of total PA and MVPA had significantly greater obesity risk, 3.0 (OR 3.0; 95% CI, 1.1–8.1; p < 0.05) and 3.8-fold (OR 3.8; 95% CI, 1.4–10.1; p < 0.01), respectively, than more active boys. Only in girls with high SB level was there a significantly increased risk of obesity, 2.9 times higher than girls at low SP levels (OR 2.8; 95% CI, 1.0–7.5; p < 0.05). Conclusions: The present findings indicate that higher PA duration and intensity reduced body fat and obesity risk while high screen-based sedentary behaviors significantly adversely influenced body fat mass, particularly amongst girls when the PA level was low.
Walkability is Only Part of the Story: Walking for Transportation in Stuttgart, Germany
Authors: Reyer Maren , Fina Stefan , Siedentop Stefan , Schlicht Wolfgang
Published: May 2014

In modern Western societies people often lead inactive and sedentary lifestyles, even though there is no doubt that physical activity and health are related. From an urban planning point of view it would be highly desirable to develop built environments in a way that supports people in leading more active and healthy lifestyles. Within this context there are several methods, predominantly used in the US, to measure the suitability of built environments for walking and cycling. Empirical studies show that people living in highly walkable areas are more physically active (for example, walk more or cycle more). The question is, however, whether these results are also valid for European cities given their different urban planning characteristics and infrastructure standards. To answer this question we used the Walkability-Index and the Walk Score to empirically investigate the associations between walkability and active transportation in the city of Stuttgart, Germany. In a sample of household survey data (n = 1.871) we found a noticeable relationship between walkability and active transportation—the more walkable an area was, the more active residents were. Although the statistical effect is small, the health impact might be of relevance. Being physically active is multi-determined and not only affected by the walkability of an area. We highlight these points with an excursion into research that the health and exercise sciences contribute to the topic. We propose to strengthen interdisciplinary research between the disciplines and to specifically collect data that captures the influence of the environment on physical activity in the future.
Drinking Water Management: Health Risk Perceptions and Choices in First Nations and Non-First Nations Communities in Canada
Authors: Dupont Diane , Waldner Cheryl , Bharadwaj Lalita , Plummer Ryan , Carter Blair , Cave Kate , Zagozewski Rebecca
Published: May 2014

The relationship between tap water and health has been a topic of public concern and calls for better management in Canada since well-publicized contamination events in two provinces (Ontario and Saskatchewan) in 2000–2001. This study reports the perspectives on health risks from tap water and corresponding use of, and spending on, bottled water in a number of different communities in Canada. In 2009–2010, four First Nations communities (three from Ontario and one from Saskatchewan) and a geographically diverse sample of non-First Nations Canadians were surveyed about their beliefs concerning health risks from tap water and their spending practices for bottled water as a substitute. Responses to five identical questions were examined, revealing that survey respondents from Ontario First Nations communities were more likely than non-First Nations Canadians to believe bottled water is safer than tap water (OR 1.6); more likely to report someone became ill from tap water (OR 3.6); more likely to express water and health concerns related to tap water consumption (OR 2.4); and more likely to spend more on bottled water (OR 4.9). On the other hand, participants from one Saskatchewan First Nations community were less likely than non-First Nations Canadians to believe that someone had become ill from drinking tap water (OR 3.8), less likely to believe bottled water is safer than tap (OR 2.0), and less likely to have health concerns with tap water (OR 1.5). These differences, however, did not translate into differences in the likelihood of high bottled water expenditures or being a 100% bottled water consumer. The paper discusses how the differences observed may be related to water supply and regulation, trust, perceived control, cultural background, location, and past experience.
A Cautionary Note on the Use of Split-YFP/BiFC in Plant Protein-Protein Interaction Studies
Authors: Horstman Anneke , Tonaco Isabella , Boutilier Kim , Immink Richard
Published: May 2014

Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions. BiFC is based on the restoration of fluorescence after the two non-fluorescent halves of a fluorescent protein are brought together by a protein-protein interaction event. The major drawback of BiFC is that the fluorescent protein halves are prone to self-assembly independent of a protein-protein interaction event. To circumvent this problem, several modifications of the technique have been suggested, but these modifications have not lead to improvements in plant BiFC protocols. Therefore, it remains crucial to include appropriate internal controls. Our literature survey of recent BiFC studies in plants shows that most studies use inappropriate controls, and a qualitative rather than quantitative read-out of fluorescence. Therefore, we provide a cautionary note and beginner’s guideline for the setup of BiFC experiments, discussing each step of the protocol, including vector choice, plant expression systems, negative controls, and signal detection. In addition, we present our experience with BiFC with respect to self-assembly, peptide linkers, and incubation temperature. With this note, we aim to provide a guideline that will improve the quality of plant BiFC experiments.
Clinical Impact of Tumor-Infiltrating Inflammatory Cells in Primary Small Cell Esophageal Carcinoma
Authors: Zhang Yuling , Ren Hongzheng , Wang Lu , Ning Zhifeng , Zhuang Yixuan , Gan Jinfeng , Chen Shaobin , Zhou David , Zhu Hua , Tan Dongfeng , Zhang Hao
Published: May 2014

Primary small cell esophageal carcinoma is a rare and aggressive type of gastrointestinal cancer with poor prognosis. In the present study, the impact of tumour infiltrating inflammatory cells on clinico-pathological characteristics and the patients’ prognosis were analysed. A total of 36 small cell esophageal carcinomas, 19 adjacent normal tissues and 16 esophageal squamous cell carcinoma samples were collected. Qualified pathologists examined eosinophils, neutrophils, lymphocytes and macrophages on histochemical slides. The infiltration of eosinophils and macrophages in small cell esophageal carcinoma was significantly increased as compared with tumor adjacent normal tissues, and was significantly less in esophageal squamous cell carcinoma. Macrophage count was significantly associated with (p = 0.015) lymph node—stage in small cell esophageal carcinoma. When we grouped patients into two groups by counts of infiltrated inflammatory cells, Kaplan-Meier analysis revealed that high macrophage infiltration group (p = 0.004) and high eosinophil infiltration group (p = 0.027) had significantly enhanced survival. In addition, multivariate analysis unveiled that eosinophil count (p = 0.002) and chemotherapy (Yes vs. No, p = 0.001) were independent prognostic indicators. Taken together, infiltration of macrophages and eosinophils into the solid tumor appear to be important in the progression of small cell esophageal carcinoma and patients’ prognosis.
Genotypic Characterization of Escherichia coli O157:H7 Isolates from Different Sources in the North-West Province, South Africa, Using Enterobacterial Repetitive Intergenic Consensus PCR Analysis
Authors: Ateba Collins , Mbewe Moses
Published: May 2014

In many developing countries, proper hygiene is not strictly implemented when animals are slaughtered and meat products become contaminated. Contaminated meat may contain Escherichia coli (E. coli) O157:H7 that could cause diseases in humans if these food products are consumed undercooked. In the present study, a total of 94 confirmed E. coli O157:H7 isolates were subjected to the enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR) typing to generate genetic fingerprints. The ERIC fragments were resolved by electrophoresis on 2% (w/v) agarose gels. The presence, absence and intensity of band data were obtained, exported to Microsoft Excel (Microsoft Office 2003) and used to generate a data matrix. The unweighted pair group method with arithmetic mean (UPGMA) and complete linkage algorithms were used to analyze the percentage of similarity and matrix data. Relationships between the various profiles and/or lanes were expressed as dendrograms. Data from groups of related lanes were compiled and reported on cluster tables. ERIC fragments ranged from one to 15 per isolate, and their sizes varied from 0.25 to 0.771 kb. A large proportion of the isolates produced an ERIC banding pattern with three duplets ranging in sizes from 0.408 to 0.628 kb. Eight major clusters (I–VIII) were identified. Overall, the remarkable similarities (72% to 91%) between the ERIC profiles for the isolate from animal species and their corresponding food products indicated some form of contamination, which may not exclude those at the level of the abattoirs. These results reveal that ERIC PCR analysis can be reliable in comparing the genetic profiles of E. coli O157:H7 from different sources in the North-West Province of South Africa.
Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury
Authors: Wang Zheng , Guo Qiong-Ya , Zhang Xiao-Ju , Li Xiao , Li Wen-Ting , Ma Xi-Tao , Ma Li-Jun
Published: May 2014

Idiopathic pulmonary fibrosis (IPF) is a progressing lethal disease with few clinically effective therapies. Corilagin is a tannin derivative which shows anti-inflammatory and antifibrotics properties and is potentiated in treating IPF. Here, we investigated the effect of corilagin on lung injury following bleomycin exposure in an animal model of pulmonary fibrosis. Corilagin abrogated bleomycin-induced lung fibrosis as assessed by H&E; Masson’s trichrome staining and lung hydroxyproline content in lung tissue. Corilagin reduced the number of apoptotic lung cells and prevented lung epithelial cells from membrane breakdown, effluence of lamellar bodies and thickening of the respiratory membrane. Bleomycin exposure induced expression of MDA, IKKα, phosphorylated IKKα (p-IKKα), NF-κB P65, TNF-α and IL-1β, and reduced I-κB expression in mice lung tissue or in BALF. These changes were reversed by high-dose corilagin (100 mg/kg i.p) more dramatically than by low dose (10 mg/kg i.p). Last, corilagin inhibits TGF-β1 production and α-SMA expression in lung tissue samples. Taken together, these findings confirmed that corilagin attenuates bleomycin-induced epithelial injury and fibrosis via inactivation of oxidative stress, proinflammatory cytokine release and NF-κB and TGF-β1 signaling. Corilagin may serve as a promising therapeutic agent for pulmonary fibrosis.
Stem Cells toward the Future: The Space Challenge
Authors: Bradamante Silvia , Barenghi Livia , Maier Jeanette
Published: May 2014

Astronauts experience weightlessness-induced bone loss due to an unbalanced process of bone remodeling that involves bone mesenchymal stem cells (bMSCs), as well as osteoblasts, osteocytes, and osteoclasts. The effects of microgravity on osteo-cells have been extensively studied, but it is only recently that consideration has been given to the role of bone MSCs. These live in adult bone marrow niches, are characterized by their self-renewal and multipotent differentiation capacities, and the published data indicate that they may lead to interesting returns in the biomedical/bioengineering fields. This review describes the published findings concerning bMSCs exposed to simulated/real microgravity, mainly concentrating on how mechanosignaling, mechanotransduction and oxygen influence their proliferation, senescence and differentiation. A comprehensive understanding of bMSC behavior in microgravity and their role in preventing bone loss will be essential for entering the future age of long-lasting, manned space exploration.
4-[5-(2-Methoxyphenyl)-1,3,4-oxadiazol-2-yl]benzohydrazide
Authors: Taha Muhammad , Ismail Nor , Imran Syahrul , Khan Khalid
Published: May 2014

4-(5-(2-Methoxyphenyl)-1,3,4-oxadiazol-2-yl)benzohydrazide (5) was synthesized by three steps. The synthesis started with 2-methoxybenzohydrazide to form hydrazone (3) which was then cyclized to oxadiazole (4) and finally, treatment of oxadiazole (4) with hydrazine hydrate afforded the final product (5).
Potassium {4-[(3S,6S,9S)-3,6-dibenzyl-9-isopropyl-4,7,10-trioxo-11–oxa-2,5,8-triazadodecyl]phenyl}trifluoroborate
Authors: Tsai Chia-Hua , Lin Chia-Hung , Hsieh Ching-Tien , Cai Chih-Cheng , Lin Ting-Ju , Liu Pin-Yi , Lin Meng-Hsuan , Wu Meng-Ju , Fu Chia-Chieh , Wu Yang-Chang , Chang Fang-Rong , Pan Po-Shen
Published: May 2014

The reported compound 4 was synthesized and fully characterized by 1H NMR, 13C NMR, 11B NMR, 19F NMR, and high resolution mass spectrometry.
Comparison of the Nanostructure and Mechanical Performance of Highly Exfoliated Epoxy-Clay Nanocomposites Prepared by Three Different Protocols
Authors: Shiravand Fatemeh , Hutchinson John , Calventus Yolanda , Ferrando Francesc
Published: May 2014

Three different protocols for the preparation of polymer layered silicate nanocomposites based upon a tri-functional epoxy resin, triglycidyl para-amino phenol (TGAP), have been compared in respect of the cure kinetics, the nanostructure and their mechanical properties. The three preparation procedures involve 2 wt% and 5 wt% of organically modified montmorillonite (MMT), and are: isothermal cure at selected temperatures; pre-conditioning of the resin-clay mixture before isothermal cure; incorporation of an initiator of cationic homopolymerisation, a boron tri-fluoride methyl amine complex, BF3·MEA, within the clay galleries. It was found that features of the cure kinetics and of the nanostructure correlate with the measured impact strength of the cured nanocomposites, which increases as the degree of exfoliation of the MMT is improved. The best protocol for toughening the TGAP/MMT nanocomposites is by the incorporation of 1 wt% BF3·MEA into the clay galleries of nanocomposites containing 2 wt% MMT.
Janus Nematic Colloids with Designable Valence
Authors: Čopar Simon , Ravnik Miha , Žumer Slobodan
Published: May 2014

Generalized Janus nematic colloids based on various morphologies of particle surface patches imposing homeotropic and planar surface anchoring are demonstrated. By using mesoscopic numerical modeling, multiple types of Janus particles are explored, demonstrating a variety of novel complex colloidal structures. We also show binding of Janus particles to a fixed Janus post in the nematic cell, which acts as a seed and a micro-anchor for the colloidal structure. Janus colloidal structures reveal diverse topological defect configurations, which are effectively combinations of surface boojum and bulk defects. Topological analysis is applied to defects, importantly showing that topological charge is not a well determined topological invariant in such patchy nematic Janus colloids. Finally, this work demonstrates colloidal structures with designable valence, which could allow for targeted and valence-conditioned self-assembly at micro- and nano-scale.
Reliable Metabolic Flux Estimation in Escherichia coli Central Carbon Metabolism Using Intracellular Free Amino Acids
Authors: Okahashi Nobuyuki , Kajihata Shuichi , Furusawa Chikara , Shimizu Hiroshi
Published: May 2014

13C metabolic flux analysis (MFA) is a tool of metabolic engineering for investigation of in vivo flux distribution. A direct 13C enrichment analysis of intracellular free amino acids (FAAs) is expected to reduce time for labeling experiments of the MFA. Measurable FAAs should, however, vary among the MFA experiments since the pool sizes of intracellular free metabolites depend on cellular metabolic conditions. In this study, minimal 13C enrichment data of FAAs was investigated to perform the FAAs-based MFA. An examination of a continuous culture of Escherichia coli using 13C-labeled glucose showed that the time required to reach an isotopically steady state for FAAs is rather faster than that for conventional method using proteinogenic amino acids (PAAs). Considering 95% confidence intervals, it was found that the metabolic flux distribution estimated using FAAs has a similar reliability to that of the PAAs-based method. The comparative analysis identified glutamate, aspartate, alanine and phenylalanine as the common amino acids observed in E. coli under different culture conditions. The results of MFA also demonstrated that the 13C enrichment data of the four amino acids is required for a reliable analysis of the flux distribution.
Metabolome Consistency: Additional Parazoanthines from the Mediterranean Zoanthid Parazoanthus Axinellae
Authors: Audoin Coralie , Cocandeau Vincent , Thomas Olivier , Bruschini Adrien , Holderith Serge , Genta-Jouve Grégory
Published: May 2014

Ultra-high pressure liquid chromatography coupled to high resolution mass spectrometry (UHPLC-MS/MS) analysis of the organic extract obtained from the Mediterranean zoanthid Parazoanthus axinellae yielded to the identification of five new parazoanthines F-J. The structures were fully determined by comparison of fragmentation patterns with those of previously isolated parazoathines and MS/MS spectra simulation of in silico predicted compounds according to the metabolome consistency. The absolute configuration of the new compounds has been assigned using on-line electronic circular dichroism (UHPLC-ECD). We thus demonstrated the potential of highly sensitive hyphenated techniques to characterize the structures of a whole family of natural products within the metabolome of a marine species. Minor compounds can be characterized using these techniques thus avoiding long isolation processes that may alter the structure of the natural products. These results are also of interest to identify putative bioactive compounds present at low concentration in a complex mixture.
Jarosite versus Soluble Iron-Sulfate Formation and Their Role in Acid Mine Drainage Formation at the Pan de Azúcar Mine Tailings (Zn-Pb-Ag), NW Argentina
Authors: Murray Jesica , Kirschbaum Alicia , Dold Bernhard , Guimaraes Edi , Miner Elisa
Published: May 2014

Secondary jarosite and water-soluble iron-sulfate minerals control the composition of acid mine waters formed by the oxidation of sulfide in tailings impoundments at the (Zn-Pb-Ag) Pan de Azúcar mine located in the Pozuelos Lagoon Basin (semi-arid climate) in Northwest (NW) Argentina. In the primary zone of the tailings (9.5 wt % pyrite-marcasite) precipitation of anglesite (PbSO4), wupatkite ((Co,Mg,Ni)Al2(SO4)4) and gypsum retain Pb, Co and Ca, while mainly Fe2+, Zn2+, Al3+, Mg2+, As3+/5+ and Cd2+ migrate downwards, forming a sulfate and metal-rich plume. In the oxidation zone, jarosite (MFe3(TO4)2(OH)6) is the main secondary Fe3+ phase; its most suitable composition is M = K+, Na+, and Pb2+and TO4 = SO42−; AsO42−. During the dry season, iron-sulfate salts precipitate by capillary transport on the tailings and at the foot of DC2 (tailings impoundment DC2) tailings dam where an acid, Fe2+ rich plume outcrops. The most abundant compounds in the acid mine drainage (AMD) are SO42−, Fe2+, Fe3+, Zn2+, Al3+, Mg2+, Cu2+, As3+/5+, Cd2+. These show peak concentrations at the beginning of the wet season, when the soluble salts and jarosite dissolve. The formation of soluble sulfate salts during the dry season and dilution during the wet season conform an annual cycle of rapid metals and acidity transference from the tailings to the downstream environment.
Synthesis and Cytotoxicity of 2,3-Enopyranosyl C-Linked Conjugates of Genistein
Authors: Szeja Wieslaw , Grynkiewicz Grzegorz , Bieg Tadeusz , Swierk Piotr , Byczek Anna , Papaj Katarzyna , Kitel Radosław , Rusin Aleksandra
Published: May 2014

A series of glycoconjugates, derivatives of genistein containing a C-glycosylated carbohydrate moiety, were synthesized and their anticancer activity was tested in vitro in the human cell lines HCT 116 and DU 145. The target compounds 15–17 were synthesized by treating ω-bromoalkyl C-glycosides derived from L-rhamnal (1) with a tetrabutylammonium salt of genistein. The new, metabolically stable analogs of previously studied O-glycosidic genistein derivatives inhibited proliferation of cancer cell lines through inhibition of the cell cycle.
Diethyl 2-(Phenylcarbamoyl)phenyl Phosphorothioates: Synthesis, Antimycobacterial Activity and Cholinesterase Inhibition
Authors: Vinšová Jarmila , Krátký Martin , Komlóová Markéta , Dadapeer Echchukattula , Štěpánková Šárka , Vorčáková Katarína , Stolaříková Jiřina
Published: May 2014

A new series of 27 diethyl 2-(phenylcarbamoyl)phenyl phosphorothioates (thiophosphates) was synthesized, characterized by NMR, IR and CHN analyses and evaluated against Mycobacterium tuberculosis H37Rv, Mycobacterium avium and two strains of Mycobacterium kansasii. The best activity against M. tuberculosis was found for O-{4-bromo-2-[(3,4-dichlorophenyl)carbamoyl]phenyl} O,O-diethyl phosphorothioate (minimum inhibitory concentration of 4 µM). The highest activity against nontuberculous mycobacteria was exhibited by O-(5-chloro-2-{[4-(trifluoromethyl)phenyl]carbamoyl}-phenyl) O,O-diethyl phosphorothioate with MIC values from 16 µM. Prepared thiophosphates were also evaluated against acetylcholinesterase from electric eel and butyrylcholinesterase from equine serum. Their inhibitory activity was compared to that of the known cholinesterases inhibitors galanthamine and rivastigmine. All tested compounds showed a higher (for AChE inhibition) and comparable (for BChE inhibition) activity to that of rivastigmine, with IC50s within the 8.04 to 20.2 µM range.
Bis-naphtho-γ-pyrones from Fungi and Their Bioactivities
Authors: Lu Shiqiong , Tian Jin , Sun Weibo , Meng Jiajia , Wang Xiaohan , Fu Xiaoxiang , Wang Ali , Lai Daowan , Liu Yang , Zhou Ligang
Published: May 2014

Bis-naphtho-γ-pyrones are an important group of aromatic polyketides derived from fungi. They have a variety of biological activities including cytotoxic, antitumor, antimicrobial, tyrosine kinase and HIV-1 integrase inhibition properties, demonstrating their potential applications in medicine and agriculture. At least 59 bis-naphtho-γ-pyrones from fungi have been reported in the past few decades. This mini-review aims to briefly summarize their occurrence, biosynthesis, and structure, as well as their biological activities. Some considerations regarding to synthesis, production, and medicinal and agricultural applications of bis-naphtho-γ-pyrones are also discussed.
Characterization of Drought Development through Remote Sensing: A Case Study in Central Yunnan, China
Authors: Abbas Sawaid , Nichol Janet , Qamer Faisal , Xu Jianchu
Published: May 2014

This study assesses the applicability of remote sensing data for retrieval of key drought indicators including the degree of moisture deficiency, drought duration and areal extent of drought within different land cover types across the landscape. A Normalized Vegetation Supply Water Index (NVSWI) is devised, combining remotely sensed climate data to retrieve key drought indicators over different vegetation cover types and a lag-time relationship is established based on preceding rainfall. The results indicate that during the major drought event of spring 2010, Evergreen Forest (EF) experienced severe dry conditions for 48 days fewer than Cropland (CL) and Shrubland (SL). Testing of vegetation response to drought conditions with different lag-time periods since the last rainfall indicated a highest correlation for CL and SL with the 4th lag period (i.e., 64 days) whereas EF exhibited maximum correlation with the 5th lag period (i.e., 80 days). Evergreen Forest, which includes tree crops, appears to act as a green reservoir of water, and is more resistant than CL and SL to drought due to its water retention capacity with deeper roots to tap sub-surface water. Identifying differences in rainfall lag-time relationships among land cover types using a remote sensing-based integrated drought index enables more accurate drought prediction, and can thus assist in the development of more specific drought adaptation strategies.
Physical, Bio-Optical State and Correlations in North–Western European Shelf Seas
Authors: Garaba Shungudzemwoyo , Voß Daniela , Zielinski Oliver
Published: May 2014

Color of seawater has become an integral tool in understanding surface marine ecosystems and processes. In this paper we seek to assess the correlations and consequently the potential of using shipborne remote sensing products to infer marine environmental parameters. Typical seawater parameters are chlorophyll–a (chl–a), colored dissolved organic material (CDOM), suspended particulate material (SPM), Secchi–disk depth (SDD), temperature, and salinity. These parameters and radiometric quantities were observed from a total of 60 stations covering German Bight, North Sea, Inner Seas, Irish Sea, and Celtic Sea. Bio-optical models developed in this study were used to predict the in situ measured parameters, with low mean unbiased percent differences and absolute percent difference less than 35%. Our investigations show that the use of ocean color products namely the Forel–Ule Index to infer seawater parameters is encouraging. The constrained spatial and temporal span of measured in situ parameters does limit the accuracy of our models. Absorption coefficients of the main color producing agents CDOM, chl–a, and inorganic fraction of SPM (iSPM) were determined to estimate absorption budgets. During the field campaign, iSPM was the primary light absorber over the spectral range (400–700 nm) although variabilities were observed in the regional seas.
Precise Calibration of a GNSS Antenna Array for Adaptive Beamforming Applications
Authors: Daneshmand Saeed , Sokhandan Negin , Zaeri-Amirani Mohammad , Lachapelle Gérard
Published: May 2014

The use of global navigation satellite system (GNSS) antenna arrays for applications such as interference counter-measure, attitude determination and signal-to-noise ratio (SNR) enhancement is attracting significant attention. However, precise antenna array calibration remains a major challenge. This paper proposes a new method for calibrating a GNSS antenna array using live signals and an inertial measurement unit (IMU). Moreover, a second method that employs the calibration results for the estimation of steering vectors is also proposed. These two methods are applied to the receiver in two modes, namely calibration and operation. In the calibration mode, a two-stage optimization for precise calibration is used; in the first stage, constant uncertainties are estimated while in the second stage, the dependency of each antenna element gain and phase patterns to the received signal direction of arrival (DOA) is considered for refined calibration. In the operation mode, a low-complexity iterative and fast-converging method is applied to estimate the satellite signal steering vectors using the calibration results. This makes the technique suitable for real-time applications employing a precisely calibrated antenna array. The proposed calibration method is applied to GPS signals to verify its applicability and assess its performance. Furthermore, the data set is used to evaluate the proposed iterative method in the receiver operation mode for two different applications, namely attitude determination and SNR enhancement.
Analysis of Benzo[a]pyrene in Vegetable Oils Using Molecularly Imprinted Solid Phase Extraction (MISPE) Coupled with Enzyme-Linked Immunosorbent Assay (ELISA)
Authors: Pschenitza Michael , Hackenberg Rudolf , Niessner Reinhard , Knopp Dietmar
Published: May 2014

This paper describes the development of a molecularly imprinted polymer-based solid phase extraction (MISPE) method coupled with enzyme-linked immunosorbent assay (ELISA) for determination of the PAH benzo[a]pyrene (B[a]P) in vegetable oils. Different molecularly imprinted polymers (MIPs) were prepared using non-covalent 4-vinylpyridine/divinylbenzene co-polymerization at different ratios and dichloromethane as porogen. Imprinting was done with a template mixture of phenanthrene and pyrene yielding a broad-specific polymer for PAHs with a maximum binding capacity (Q) of ~32 μg B[a]P per 50 mg of polymer. The vegetable oil/n-hexane mixture (1:1, (v/v)) was pre-extracted with acetonitrile, the solvent evaporated, the residue reconstituted in n-hexane and subjected to MISPE. The successive washing with n-hexane and isopropanol revealed most suitable to remove lipid matrix constituents. After elution of bound PAHs from MISPE column with dichloromethane, the solvent was evaporated, the residue reconstituted with dimethyl sulfoxide and diluted 100-fold with methanol/water (10:90, (v/v)) for analysis of B[a]P equivalents with an ELISA. The B[a]P recovery rates in spiked vegetable oil samples of different fatty acid composition were determined between 63% and 114%. The presence of multiple PAHs in the oil sample, because of MIP selectivity and cross-reactivity of the ELISA, could yield overestimated B[a]P values.
A Multi-Scalar Examination of Law for Sustainable Ecosystems
Authors: Green Olivia , Garmestani Ahjond , Hopton Matthew , Heberling Matthew
Published: May 2014

The loss of resilience in social-ecological systems has the capacity to decrease essential ecosystem services, posing threats to human survival. To achieve sustainability, we must not only understand the ecological dynamics of a system, such as coral reefs, but must also promulgate regulations that promote beneficial behavior to address ecological stressors throughout the system. Furthermore, laws should reflect that systems operate at multiple spatial and temporal scales, thus requiring management across traditional legal jurisdictions. In this paper, we conducted a multi-scalar examination of law for sustainable ecosystems and how law pertains to coral reef ecosystems in particular. Findings indicate that, in order to achieve sustainability, we must develop new or reform existing legal mechanisms to protect ecosystems.
Clustering of Giant Virus-DNA Based on Variations in Local Entropy
Authors: Bose Ranjan , Thiel Gerhard , Hamacher Kay
Published: May 2014

We present a method for clustering genomic sequences based on variations in local entropy. We have analyzed the distributions of the block entropies of viruses and plant genomes. A distinct pattern for viruses and plant genomes is observed. These distributions, which describe the local entropic variability of the genomes, are used for clustering the genomes based on the Jensen-Shannon (JS) distances. The analysis of the JS distances between all genomes that infect the chlorella algae shows the host specificity of the viruses. We illustrate the efficacy of this entropy-based clustering technique by the segregation of plant and virus genomes into separate bins.
Future Climate Forcings and Olive Yield in a Mediterranean Orchard
Authors: Viola Francesco , Caracciolo Domenico , Pumo Dario , Noto Leonardo , Loggia Goffredo
Published: May 2014

The olive tree is one of the most characteristic rainfed trees in the Mediterranean region. Observed and forecasted climate modifications in this region, such as the CO2 concentration and temperature increase and the net radiation, rainfall and wind speed decrease, will likely alter vegetation water stress and modify productivity. In order to simulate how climatic change could alter soil moisture dynamic, biomass growth and fruit productivity, a water-driven crop model has been used in this study. The numerical model, previously calibrated on an olive orchard located in Sicily (Italy) with a satisfactory reproduction of historical olive yield data, has been forced with future climate scenarios generated using a stochastic weather generator and a downscaling procedure of an ensemble of climate model outputs. The stochastic downscaling is carried out using simulations of some General Circulation Models adopted in the fourth Intergovernmental Panel on Climate Change (IPCC) assessment report (4AR) for future scenarios. The outcomes state that climatic forcings driving potential evapotranspiration compensate for each other, resulting in a slight increase of this water demand flux; moreover, the increase of CO2 concentration leads to a potential assimilation increase and, consequently, to an overall productivity increase in spite of the growth of water stress due to the rainfall reduction.
Teoría sintética: crisis y revolución
Authors: Sendín Máximo
Published: May 2014

Desde su mismo nacimiento, la teoría darwinista adolecía de notorias lagunas que eran reconocidas por su autor. Tanto la observación de las especies naturales como los datos del registro fósil mostraban patentes discrepancias con dos de sus conceptos centrales: la selección natural y el cambio gradual, problemas que inquietaban profundamente a Darwin y a algunos de sus seguidores.
Pero estos problemas, claramente observables, fueron «resueltos» de una forma teórica por los modelos matemáticos de la genética de poblaciones, con lo que, a mediados de este siglo, el darwinismo se consolidó en forma de teoría sintética moderna, modelo evolutivo aceptado mayoritariamente, desde entonces, por la comunidad científica.
Mientras tanto, observaciones contemporáneas provenientes del campo de la embriología sumaban nuevas discrepancias que contribuían a una creciente divergencia entre los datos observados y el modelo teórico.
Esta discrepancia ha llegado a su punto máximo a partir de los recientes descubrimientos de la genética molecular y, especialmente, de la genética del desarrollo. La implicación de elementos móviles, virus endógenos, secuencias repetidas, genes homeóticos... en la transmisión de información genética, y la complejidad de su actuación durante el desarrollo embrionario, ha convertido dicha divergencia en una abierta contradicción.
La situación a que han conducido a la Biología estas evidencias contradictorias con su modelo teórico fundamental se corresponde con lo que Thomas Kuhn define como crisis en la ciencia.
En este contexto, los crecientes indicios de un origen viral para las secuencias antes citadas, junto con la capacidad de los virus de integrarse en los genomas animales y vegetales, aportaría un mecanismo evolutivo de carácter infectivo susceptible de dar respuesta a los problemas antes mencionados.
La confirmación de esta hipótesis constituiría lo que para Kuhn es una «revolución científica» y el subsiguiente cambio de paradigma, ya que afectaría no sólo al mecanismo evolutivo, sino también a su interpretación y significado.
Sujata Bhatt: los colores del encuentro
Authors: Janés Nadal Clara
Published: May 2014

Invitada al Festival de Poesía de Bremen, movida por la alegría de ir a Alemania cuando el último poeta amado era un alemán, Johannes Bobrowski, confesé este hecho en público. Se me acercó entonces Sujata Bahtt, poetisa india que también admira a este poeta, e iniciamos un hermoso intercambio. Al día siguiente me regaló sus libros, donde se trasluce el contraste entre las culturas oriental y occidental. En Bremen escribí yo un largo poema-crónica con trasfondos y citas múltiples. Luego traduje uno de los libros de Sujata y lo presentamos en Cartagena. Meses después nos encontramos en Delhi. Finalmente nos volvimos a reunir en Bremen, de nuevo en el Festival. una sesión para las dos solas en el Museo Paula Modersohn-Becker, donde ella presentó un nuevo libro puesto en boca de esa pintora amiga de rilke, yo el poema-crónica donde hablo de poesía, de la ciudad y cito a rilke.
Assia Djebar, la lengua del enemigo
Authors: Jiménez Morell Inmaculada
Published: May 2014

Assia Djebar, escritora argelina en lengua francesa, «la lengua del enemigo», es miembro desde 2006 de la Academia Francesa y Premio de la Paz (2000) otorgado por los libreros y editores alemanes.
Zadie Smith: un homenaje
Authors: Rivera de la Cruz Marta
Published: May 2014

Considerada por la prestigiosa revista Granta uno de los nombres propios de las letras anglosajonas, Zadie Smith irrumpió en la escena literaria con Dientes blancos cuando contaba con poco más de veinte años. Su extraordinario talento unido a su extrema juventud sorprendieron a la crítica, pero también al público y a los medios de comunicación, que no tardaron en hacer de ella un nuevo icono de la literatura moderna. Zadie Smith ha pasado los últimos años escribiendo y huyendo a la vez de la feria de vanidades de la fama, que amenazaba con oscurecer sus dotes de narradora. Es una escritora total, cuya tercera novela, Sobre la belleza, la ha consagrado de forma definitiva.