bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Allergenic Proteins in Foodstuffs: Advantages of the Innovative Multiplex Allergen Microarray-Based Immunoassay Compared to Conventional Methods
Authors: Tuppo Lisa , Giangrieco Ivana , Tamburrini Maurizio , Alessandri Claudia , Mari Adriano , Ciardiello Maria Antonietta
Published: March 2022

Several factors can affect the allergen content and profile of a specific food, including processing procedures often leading to a decrease in allergenicity, although no change, or even an increase, have also been reported. Evaluation of the effectiveness of a processing procedure requires the availability of reliable methodologies to assess the variation in molecules able to induce allergic reactions in the analyzed food. Conventional and innovative strategies and methodologies can be exploited to identify allergenic proteins in foodstuffs. However, depending on the specific purposes, different methods can be used. In this review, we have critically reviewed the advantages of an innovative method, the multiplex allergen microarray-based immunoassay, in the detection of allergens in foodstuffs. In particular, we have analyzed some studies reporting the exploitation of an IgE-binding inhibition assay on multiplex allergen biochips, which has not yet been reviewed in the available literature. Unlike the others, this methodology enables the identification of many allergenic proteins, some of which are still unknown, which are recognized by IgE from allergic patients, with a single test. The examined literature suggests that the inhibition test associated with the multiplex allergen immunoassay is a promising methodology exploitable for the detection of IgE-binding proteins in food samples.
Salmonella spp. Response to Lytic Bacteriophage and Lactic Acid on Marinated and Tenderized Raw Pork Loins
Authors: Li Sherita , Konoval Haley M. , Marecek Samantha , Lathrop Amanda A. , Pokharel Siroj
Published: March 2022

Bacterial food poisoning cases due to Salmonella have been linked with a variety of pork products. This study evaluated the effects of a Salmonella-specific lytic bacteriophage and lactic acid (LA) on Salmonella Enteritidis, Salmonella Montevideo, and Salmonella Heidelberg growth on raw pork loins. Pork loins were cut into approximately 4 cm thick slices. Pork slices were randomly assigned to five treatment groups (control, DI water, LA 2.5%, phage 5%, and LA 2.5% + phage 5%) with six slices per group per replication. Pork loins were inoculated with 106 CFU/mL of Salmonella spp. and stored at 4 °C for 30 min. After 1 h of treatment application and marination, phage 5% significantly (p < 0.05) reduced the surface bacterial population by 2.30 logs when compared with the control group. Moreover, the combined treatment of LA 2.5% + phage 5% significantly (p < 0.05) reduced the surface bacterial population by more than 2.36 logs after 1 h of marination. In the post-tenderization surface samples, the combination of both phage and LA showed a significant reduction (p < 0.05) when compared with the control group. However, the treatments had no effect (p > 0.05) when analyzing the translocation of pathogens on pork loins.
Effects of Ultrasonic Treatment on the Structure, Functional Properties of Chickpea Protein Isolate and Its Digestibility In Vitro
Authors: Kang Shihua , Zhang Jian , Guo Xiaobing , Lei Yongdong , Yang Mei
Published: March 2022

This study evaluated the effects of different levels of ultrasonic power (200, 400, 600 W) and treatment time (0, 10, 15 and 30 min) on the structure, emulsification characteristics, and in vitro digestibility of chickpea protein isolate (CPI). The changes in surface hydrophobicity of CPI indicated that ultrasound treatment exposed more hydrophobic amino acid residues. The analysis of sulfhydryl content and zeta potential showed that ultrasound caused the disulfide bond of CPI to be opened, releasing more negatively charged groups, and the solution was more stable. In addition, Fourier Transform Infrared Spectroscopy (FT-IR) and intrinsic fluorescence spectroscopy showed that ultrasound changes the secondary and tertiary structure of CPI, which is due to molecular expansion and stretching, exposing internal hydrophobic groups. The emulsification and foaming stability of CPI were significantly improved after ultrasonic treatment. Ultrasonic treatment had a minor effect on the solubility, foaming capacity and in vitro digestibility of CPI. All the results revealed that the ultrasound was a promising way to improve the functional properties of CPI.
A Hybrid RSM-ANN-GA Approach on Optimization of Ultrasound-Assisted Extraction Conditions for Bioactive Component-Rich Stevia rebaudiana (Bertoni) Leaves Extract
Authors: Ameer Kashif , Ameer Saqib , Kim Young-Min , Nadeem Muhammad , Park Mi-Kyung , Murtaza Mian Anjum , Khan Muhammad Asif , Nasir Muhammad Adnan , Mueen-ud-Din Ghulam , Mahmood Shahid , Kausar Tusneem , Abubakar Muhammad
Published: March 2022

Stevia rebaudiana (Bertoni) leaves consist of dietetically important diterpene steviol glycosides (SGs): stevioside (ST) and rebaudioside-A (Reb-A). ST and Reb-A are key sweetening compounds exhibiting a sweetening potential of 100 to 300 times more intense than that of table sucrose. Ultrasound-assisted extraction (UAE) of SGs was optimized by effective process optimization techniques, such as response surface methodology (RSM) and artificial neural network (ANN) modeling coupled with genetic algorithm (GA) as a function of ethanol concentration (X1: 0–100%), sonication time (X2: 10–54 min), and leaf–solvent ratio (X3: 0.148–0.313 g·mL−1). The maximum target responses were obtained at optimum UAE conditions of 75% (X1), 43 min (X2), and 0.28 g·mL−1 (X3). ANN-GA as a potential alternative indicated superiority to RSM. UAE as a green technology proved superior to conventional maceration extraction (CME) with reduced resource consumption. Moreover, UAE resulted in a higher total extract yield (TEY) and SGs including Reb-A and ST yields as compared to those that were obtained by CME with a marked reduction in resource consumption and CO2 emission. The findings of the present study evidenced the significance of UAE as an ecofriendly extraction method for extracting SGs, and UAE scale-up could be employed for effectiveness on an industrial scale. These findings evidenced that the UAE is a high-efficiency extraction method with an improved statistical approach.
Effects of Radio Frequency Tempering on the Temperature Distribution and Physiochemical Properties of Salmon (Salmo salar)
Authors: Han Rong , He Jialing , Chen Yixuan , Li Feng , Shi Hu , Jiao Yang
Published: March 2022

Salmon (Salmo salar) is a precious fish with high nutritional value, which is perishable when subjected to improper tempering processes before consumption. In traditional air and water tempering, the medium temperature of 10 °C is commonly used to guarantee a reasonable tempering time and product quality. Radio frequency tempering (RT) is a dielectric heating method, which has the advantage of uniform heating to ensure meat quality. The effects of radio frequency tempering (RT, 40.68 MHz, 400 W), water tempering (WT + 10 °C, 10 ± 0.5 °C), and air tempering (AT + 10 °C, 10 ± 1 °C) on the physiochemical properties of salmon fillets were investigated in this study. The quality of salmon fillets was evaluated in terms of drip loss, cooking loss, color, water migration and texture properties. Results showed that all tempering methods affected salmon fillet quality. The tempering times of WT + 10 °C and AT + 10 °C were 3.0 and 12.8 times longer than that of RT, respectively. AT + 10 °C produced the most uniform temperature distribution, followed by WT + 10 °C and RT. The amount of immobile water shifting to free water after WT + 10 °C was higher than that of RT and AT + 10 °C, which was in consistent with the drip and cooking loss. The spaces between the intercellular fibers increased significantly after WT + 10 °C compared to those of RT and AT + 10 °C. The results demonstrated that RT was an alternative novel salmon tempering method, which was fast and relatively uniform with a high quality retention rate. It could be applied to frozen salmon fillets after receiving from overseas catches, which need temperature elevation for further cutting or consumption.
Determination of Mycotoxins in Dried Fruits Using LC-MS/MS—A Sample Homogeneity, Troubleshooting and Confirmation of Identity Study
Authors: Zhang Kai , Tan Steven , Xu David
Published: March 2022

To monitor co-exposure to toxic mycotoxins in dried fruits, it is advantageous to simultaneously determine multiple mycotoxins using a single extraction and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. In this study, we applied a stable isotope dilution and LC-MS/MS method to multi-mycotoxin analysis in dried fruits, selecting raisins, plums, figs, and cranberries for matrix extension. Samples were prepared using cryogenic grinding, followed by the fortification of carbon-13 (13C) uniformly labeled internal standards for twelve mycotoxins, and extraction using 50% acetonitrile. Homogeneity of prepared samples, defined as particle size Dv90 < 850 µm for the tested matrices, was characterized using a laser diffraction particle size analyzer, and reached using cryogenic grinding procedures. The majority of recoveries in the four matrices for aflatoxins and ochratoxin A spiked at 1–100 ng/g; fumonisins, T-2 toxin, HT-2 toxin, and zearalenone spiked at 10–1000 ng/g, ranged from 80 to 120% with relative standard deviations (RSDs) of <20%. Deoxynivalenol was not detected at 10 and 100 ng/g in plums, and additional troubleshooting procedures using liquid-liquid extraction (LLE), solid phase extraction (SPE), and elution gradient were evaluated to improve the detectability of the mycotoxin. Furthermore, we confirmed the identity of detected mycotoxins, ochratoxin A and deoxynivalenol, in incurred samples using enhanced product ion scans and spectral library matching.
New Exact Solutions of Some Important Nonlinear Fractional Partial Differential Equations with Beta Derivative
Authors: Ozkan Erdogan Mehmet
Published: March 2022

In this work, the F-expansion method is used to find exact solutions of the space-time fractional modified Benjamin Bona Mahony equation and the nonlinear time fractional Schrödinger equation with beta derivative. One of the most efficient and significant methods for obtaining new exact solutions to nonlinear equations is this method. With the aid of Maple, more exact solutions defined by the Jacobi elliptic function are obtained. Hyperbolic function solutions and some exact solutions expressed by trigonometric functions are gained in the case of m modulus 1 and 0 limits of the Jacobi elliptic function.
Riemann–Liouville Fractional Newton’s Type Inequalities for Differentiable Convex Functions
Authors: Sitthiwirattham Thanin , Nonlaopon Kamsing , Ali Muhammad Aamir , Budak Hüseyin
Published: March 2022

In this paper, we prove some new Newton’s type inequalities for differentiable convex functions through the well-known Riemann–Liouville fractional integrals. Moreover, we prove some inequalities of Riemann–Liouville fractional Newton’s type for functions of bounded variation. It is also shown that the newly established inequalities are the extension of comparable inequalities inside the literature. Finally, we give some examples with graphs and show the validity of newly established inequalities.
Arsenate Removal from Aqueous Media Using Chitosan-Magnetite Hydrogel by Batch and Fixed-Bed Columns
Authors: Verduzco-Navarro Ilse Paulina , Mendizábal Eduardo , Rivera Mayorga José Antonio , Rentería-Urquiza Maite , Gonzalez-Alvarez Alejandro , Rios-Donato Nely
Published: March 2022

The removal of arsenate ions from aqueous solutions at near-neutral pH was carried out using chitosan-magnetite (ChM) hydrogel beads in batch systems. Equilibrium isotherms and kinetic studies are reported. Obtained equilibrium and kinetic data were fitted to mathematical models, estimating model parameters by non-linear regression analysis. Langmuir model was found to best fit equilibrium data; a maximum adsorption capacity of 66.9 mg As/g was estimated at pH 7.0. Pseudo-first order kinetic model was observed to best fit kinetic data. The pH of the solution was observed to increase with increasing contact time, which is attributed to protonation of amine groups present in the hydrogel. Protonation of functional groups in the ChM sorbent yields a higher number of active sites for arsenate removal, being as this a process that can’t be overlooked in future applications of ChM hydrogel for the removal or arsenate ions. Chitosan-magnetite and ChM-arsenate interactions were determined by XPS. Arsenate removal using fixed-bed column packed with ChM was carried out, reporting a non-ideal behavior attributed to pH increase of the effluent caused by proton transfer to ChM hydrogels.
Novel Natural Glycyrrhetinic Acid-Derived Super Metal Gel and Its Highly Selective Dyes Removal
Authors: Guo Shengzhu , Su Kaize , Yang Huiji , Zheng Wende , Zhang Zhen , Ang Song , Zhang Kun , Wu Panpan
Published: March 2022

Hydrogels play important roles in function materials, especially in wastewater treatment, that could solve the problems of microbial infections and dye pollutions. Herein, a natural glycyrrhetinic acid-derived gel was successfully constructed by forming hierarchical assemblies of the glycyrrhetinic acid derivatives (GA-O-09) with Cu2+. Interestingly, the GA-O-09/Cu2+ gel exhibited Cu2+-triggered shrinkage, which was helpful for spontaneous self-demolding through the shrinkage process with a precise amount of Cu2+. Moreover, the gel showed excellent antimicrobial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) with minimum inhibitory concentrations (MICs) at 2.5 μg/mL and 5.0 μg/mL, respectively. Furthermore, the resultant GA-O-09/Cu2+ gel showed an excellent performance in dyes removal; the adsorption capacity at equilibrium (qe) could reach 82.91 mg/g according to a pseudo-second-order model, and it was better than most reported dye adsorbent materials. The experimental result suggested that the electrostatic interactions of the hydrogel with the cationic dyes and the hydrogel swelling were responsible for the possible dye removal mechanism of GA-O-09/Cu2+ gel. Therefore, our study holds the promise of a better future, for such a hydrogel could be used as an antibacterial and dye removal material.
Development and Evaluation of Ginkgo biloba/Sodium Alginate Nanocomplex Gel as a Long-Acting Formulation for Wound Healing
Authors: Md Shadab , Abdullah Samaa , Alhakamy Nabil A. , Shaik Rasheed A. , Eldakhakhny Basmah Medhat , Omar Ulfat Mohammad , Eid Basma G. , Ansari Akhalakur Rahman , Alamoudi Abdulmohsin J. , Rizg Waleed Y. , Riadi Yassine , Venkateswaran Sunil Pazhayanur , Rashid Md Abdur
Published: March 2022

The aim of the study was to develop and evaluate the Ginkgo biloba nanocomplex gel (GKNG) as a long-acting formulation for the wound healing potential. Pharmaceutical analysis showed an average particle size of 450.14 ± 36.06 nm for GKNG, zeta potential +0.012 ± 0.003 mV, and encapsulation efficiency 91 ± 1.8%. The rheological analysis also showed the optimum diffusion rate and viscosity needed for topical drug delivery. Fourier transform infrared spectroscopy (FTIR), powder X-ray diffractometry (PXRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analysis further confirmed the success of GKNG. The in vivo study showed increments in the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) and a lower level of lipid peroxidation (MDA) after GKNG treatment. The GKNG group showed upregulations in collagen type I, as alpha 1 collagen (COL1A1), and collagen type IV, as alpha 1 collagen (COL4A1). Furthermore, the in vivo study showed increments in hydroxyproline, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and transforming growth factor-beta 1 (TGF-β1) after the GKNG. Additionally, GKNG effectively increased the wound contraction compared to GK gel and sodium alginate (SA) gel. Based on the in vitro and in vivo evaluation, GKNG effectively accelerated wound healing by modulation of antioxidant enzymes, collagens, angiogenic factors, and TGF-β1.
Supramolecular Thixotropic Ionogel Electrolyte for Sodium Batteries
Authors: Chen Shipeng , Feng Li , Wang Xiaoji , Fan Yange , Ke Yubin , Hua Lin , Li Zheng , Hou Yimin , Xue Baoyu
Published: March 2022

Owing to the potential of sodium as an alternative to lithium as charge carrier, increasing attention has been focused on the development of high-performance electrolytes for Na batteries in recent years. In this regard, gel-type electrolytes, which combine the outstanding ionic conductivity of liquid electrolytes and the safety of solid electrolytes, demonstrate immense application prospects. However, most gel electrolytes not only need a number of specific techniques for molding, but also typically suffer from breakage, leading to a short service life and severe safety issues. In this study, a supramolecular thixotropic ionogel electrolyte is proposed to address these problems. This thixotropic electrolyte is formed by the supramolecular self-assembly of D-gluconic acetal-based gelator (B8) in an ionic liquid solution of a Na salt, which exhibits moldability, a high ionic conductivity, and a rapid self-healing property. The ionogel electrolyte is chemically stable to Na and exhibits a good Na+ transference number. In addition, the self-assembly mechanism of B8 and thixotropic mechanism of ionogel are investigated. The safe, low-cost and multifunctional ionogel electrolyte developed herein supports the development of future high-performance Na batteries.
Microstructure Analysis and Effects of Single and Mixed Activators on Setting Time and Strength of Coal Gangue-Based Geopolymers
Authors: Yang Xiaoyun , Zhang Yan , Lin Cheng
Published: March 2022

Geopolymer is a green non-metallic material with high strength and favorable properties in resistance to corrosion, fire, and high temperature, which makes it a potential substitute for Portland cement. The existing studies have primarily focused on the preparation of geopolymers using silico-alumina materials such as fly ash, red mud, metakaolin, volcanic ash, and blast furnace slag to develop geopolymers. This study explores the potential of using ultrafine calcined coal gangue and ground granulated blast furnace slag to develop a new geopolymer with the activation of a single activator (sodium hydroxide) or mixed activator (sodium hydroxide, liquid sodium silicate, and desulfurization gypsum). The setting time and strength of the geopolymers were investigated, followed by the mineral, functional groups, microstructure, and elements analyses using X-ray diffraction, Fourier transform infrared diffraction, scanning electron microscope, and energy dispersive spectrometer to elucidate the effect of different activators on geopolymers. The results showed that the optimum molarity of NaOH single activator was 2 mol/L, the initial setting time and final setting time were 37 min and 47 min, respectively, and the compressive and flexural strengths at 28 days were 23.2 MPa and 7.5 MPa. The optimal mixing ratio of the mixed activator was 6% desulfurization gypsum, 0.6 Na2SiO3 modulus, and 16% SS activator; the initial setting time and final setting time were 100 min and 325 min, respectively, and the compressive and flexural strengths at 28 days were 40.1 MPa and 7.8 MPa. The coal gangue geopolymers were mainly C–A–S–H, N–A-S-H, and C–N–A–S–H gels. The mixed activator tended to yield higher strengths than the single activator, the reason is that the hydration reaction was violent and produced more gels. Meanwhile, the relation between setting time and activator and the relation between strength and activator were also obtained, which provide theoretical support for predicting the setting time of coal gangue base polymer and the ratio of alkali activator for geopolymers with a certain strength.
An Expanding Toolkit for Heterochromatin Repair Studies
Authors: Rawal Chetan C. , Butova Nadejda L. , Mitra Anik , Chiolo Irene
Published: March 2022

Pericentromeric heterochromatin is mostly composed of repetitive DNA sequences prone to aberrant recombination. Cells have developed highly specialized mechanisms to enable ‘safe’ homologous recombination (HR) repair while preventing aberrant recombination in this domain. Understanding heterochromatin repair responses is essential to understanding the critical mechanisms responsible for genome integrity and tumor suppression. Here, we review the tools, approaches, and methods currently available to investigate double-strand break (DSB) repair in pericentromeric regions, and also suggest how technologies recently developed for euchromatin repair studies can be adapted to characterize responses in heterochromatin. With this ever-growing toolkit, we are witnessing exciting progress in our understanding of how the ‘dark matter’ of the genome is repaired, greatly improving our understanding of genome stability mechanisms.
SOD-1 Variants in Amyotrophic Lateral Sclerosis: Systematic Re-Evaluation According to ACMG-AMP Guidelines
Authors: Ruffo Paola , Perrone Benedetta , Conforti Francesca Luisa
Published: March 2022

Amyotrophic lateral sclerosis (ALS) is the most common type of motor neuron disease whose causes are unclear. The first ALS gene associated with the autosomal dominant form of the disease was SOD1. This gene has a high rate of rare variants, and an appropriate classification is essential for a correct ALS diagnosis. In this study, we re-evaluated the classification of all previously reported SOD1 variants (n = 202) from ALSoD, project MinE, and in-house databases by applying the ACMG-AMP criteria to ALS. New bioinformatics analysis, frequency rating, and a thorough search for functional studies were performed. We also proposed adjusting criteria strength describing how to apply them to SOD1 variants. Most of the previously reported variants have been reclassified as likely pathogenic and pathogenic based on the modified weight of the PS3 criterion, highlighting how in vivo or in vitro functional studies are determining their interpretation and classification. Furthermore, this study reveals the concordance and discordance of annotations between open databases, indicating the need for expert review to adapt the study of variants to a specific disease. Indeed, in complex diseases, such as ALS, the oligogenic inheritance, the presence of genes that act as risk factors and the reduced penetration must be considered. Overall, the diagnosis of ALS remains clinical, and improving variant classification could support genetic data as diagnostic criteria.
Telomeric Repeat-Containing RNA (TERRA): A Review of the Literature and First Assessment in Cutaneous T-Cell Lymphomas
Authors: Chebly Alain , Ropio Joana , Baldasseroni Lyla , Prochazkova-Carlotti Martina , Idrissi Yamina , Ferrer Jacky , Farra Chantal , Beylot-Barry Marie , Merlio Jean-Philippe , Chevret Edith
Published: March 2022

Telomeric Repeat-containing RNA (TERRA) are long non-coding RNAs transcribed from telomeric DNA sequences from multiple chromosome ends. Major research efforts have been made to understand TERRA roles and functions in several physiological and pathological processes. We summarize herein available data regarding TERRA’s roles in human cells and we report the first investigation in cutaneous T-cells lymphomas (CTCL) using real-time PCR. Among the TERRA analysed, our data suggest a particular role for TERRA 16p downregulation and TERRA 11q upregulation in CTCL lymphomagenesis.
Expression Analysis of the TdDRF1 Gene in Field-Grown Durum Wheat under Full and Reduced Irrigation
Authors: Latini Arianna , Cantale Cristina , Thiyagarajan Karthikeyan , Ammar Karim , Galeffi Patrizia
Published: March 2022

Some of the key genes and regulatory mechanisms controlling drought response in durum wheat have been identified. One of the major challenges for breeders is how to use this knowledge for the achievement of drought stress tolerance. In the present study, we report the expression profiles of the TdDRF1 gene, at consecutive plant growth stages, from different durum wheat genotypes evaluated in two different field environments. The expression of a possible target gene (Wdnh13) of the TdDRF1 gene was also investigated and analogies with the transcript profiles were found. The results of the qRT-PCR highlighted differences in molecular patterns, thus suggesting a genotype dependency of the TdDRF1 gene expression in response to the stress induced. Furthermore, a statistical association between the expression of TdDRF1 transcripts and agronomic traits was also performed and significant differences were found among genotypes, suggesting a relationship. One of the genotypes was found to combine molecular and agronomic characteristics.
Console-Based Mapping of Mongolia Using GMT Cartographic Scripting Toolset for Processing TerraClimate Data
Authors: Lemenkova Polina
Published: March 2022

This paper explores spatial variability of the ten climatic variables of Mongolia in 2019: average minimal and maximal temperatures, wind speed, soil moisture, downward surface shortwave radiation (DSRAD), snow water equivalent (SWE), vapor pressure deficit (VPD), vapor pressure anomaly (VAP), monthly precipitation and Palmer Drought Severity Index (PDSI). The PDSI demonstrates the simplified soil water balance estimating relative soil moisture conditions in Mongolia. The research presents mapping of the climate datasets derived from TerraClimate open source repository of the meteorological and climate measurements in NetCDF format. The methodology presented the compiled observations of Mongolia visualised by GMT coding approach using Generic Mapping Tools (GMT) cartographic scripting toolset. The results present 10 new maps of climate data over Mongolia made using automated cartographic techniques of GMT. Spatial environmental and climate analysis were conducted which determine relative distribution of PDSI and temperature extremes, precipitation and soil moisture, wind speed and DSRAD. The DSRAD showed minimum at 40 Wm−2, maximum at 113 Wm−2 in the Gobi Desert region, SWE (up to 491 mm), VAP and VPD compared with landmass parameters represent powerful cartographic tools to address complex regional climate and environmental issues in Mongolia, a country with contrasting topography, extreme climate conditions and unique environmental setting.
Salinas and “Saltscape” as a Geological Heritage with a Strong Potential for Tourism and Geoeducation
Authors: Hueso-Kortekaas Katia , Iranzo-García Emilio
Published: March 2022

Salinas and saltscapes are relevant geoheritage sites with important implications on socioeconomic activities beyond the production of salt, particularly tourism and education. As cultural landscapes, they also have implications related to the identity of their communities. This work presents the study of the patrimonialization processes of four sites in Europe (Añana in Spain, Guérande in France, Læsø in Denmark, and Sečovlje in Slovenia). Lessons obtained from these processes may contribute to the recovery and valuation of similar saltscapes and other forms of geoheritage. The study is based on interviews with relevant stakeholders, a survey of the related grey and scientific literature, and a simplified SWOT analysis. Despite their differences in historical background and current management, all four sites share features that have contributed to the success of their patrimonialization processes, such as having a dedicated entity for this purpose or being protected in some way. They also share common threats that need to be addressed, such as the banalization of the heritage discourse. Other saltscapes and geoheritage sites in general may benefit from these common features, which should serve as an inspiration and not as a template. In the end, shifting from a little-known productive, (proto-)industrial activity toward a sustainable, multifunctional landscape in which geoeducation and tourism are paramount contributes to a more resilient and educated society.
Validation of the Portuguese Version of the Fertility Adjustment Scale
Authors: Romeiro Joana , Nogueira Paulo , Caldeira Sílvia
Published: March 2022

There is an urgent need to provide healthcare professionals and midwives with validated tools as to improve fertility adjustment and promote well-being of couples with infertility. The purpose of this study was to test validity of the Fertility Adjustment Scale among people undergoing assisted reproductive techniques. A cross-sectional and methodological study was conducted, and a total of 104 Portuguese adults undergoing fertility treatment were recruited through fertility-related websites. The Fertility Adjustment Scale was administered along with the Spiritual Well-Being Questionnaire and the Resilience Scale for adults as a measure of concurrent validity. Scores revealed the sample’s lack of adjustment to fertility. A significant correlation with measures of resilience provided evidence of convergent validity. There was a significant association of fertility adjustment with time of consultation and the cause of infertility. A Fertility Adjustment Scale with six items is a reliable tool that offers early recognition of patients’ difficulties in adaptation to fertility problems during assisted reproductive techniques, which could be beneficial in not only an early recognition of healthcare intervention but of a more individualized approach to such patients.
Grounding the Body Improves Sleep Quality in Patients with Mild Alzheimer’s Disease: A Pilot Study
Authors: Lin Chien-Hung , Tseng Shih-Ting , Chuang Yao-Chung , Kuo Chun-En , Chen Nai-Ching
Published: March 2022

Background: Grounding refers to having direct contact with the Earth, such as by walking barefoot or lying on the ground. Research has found that grounding can improve inflammation, free radical damage, blood pressure, sleep quality, pain, stress, mood, and wound healing. However, there has been no research on the effect of utilizing grounding for patients with Alzheimer’s disease (AD). Thus, in this study, we investigated the effectiveness of grounding as a non-pharmacological therapy for treating sleep disturbances, anxiety, and depression in patients with mild AD. Methods: Patients with mild AD were enrolled in the study. The electrochemical analyzer CHI 1205b was employed to check the electrochemical signals at acupoints KI1 and GV16. We used the Pittsburgh Sleep Quality Index (PSQI), Beck Anxiety Inventory (BAI), and Beck Depression Inventory-II (BDI-II) to evaluate sleep quality, anxiety, and depression, respectively, at weeks 0 and 12. Results: This 12-week placebo-controlled study enrolled 22 patients, but only 15 patients completed the 12-week intervention and survey. Grounding significantly improved PSQI scores compared to the sham-grounding group (mean ± SD: 0.3 ± 0.7 vs. 3.0 ± 1.9, p = 0.006). The scores on the BAI and BDI-II did not change significantly after grounding in comparison to the sham-grounding group. Conclusions: Grounding can improve sleep quality, but it does not significantly improve anxiety and depression among patients with mild AD.
The Effect of an Optimized Diet as an Adjunct to Non-Surgical Periodontal Therapy in Subjects with Periodontitis: A Prospective Study
Authors: De Angelis Paolo , Gasparini Giulio , Manicone Paolo Francesco , Passarelli Pier Carmine , Azzolino Domenico , Rella Edoardo , De Rosa Giuseppe , Papi Piero , Pompa Giorgio , De Angelis Silvio , Grassi Roberta , D’Addona Antonio
Published: March 2022

Diet and nutrition are generally categorized as modifiable lifestyle risk factors for the development of periodontal disease because diet may influence a person’s inflammatory status. This study aimed to evaluate the efficacy of the application of a diet plan focused on reducing inflammation and oxidative stress in treating periodontitis. Subjects suffering from periodontitis were divided into two groups. Both groups underwent non-surgical periodontal therapy, and in the optimized diet (OD) group, this treatment was associated with a diet plan. The sample consisted of 60 subjects; 32 (53%) were treated in the non-optimized diet group (ND group) and 28 (47%) in the OD group. In both groups, the periodontal treatment significantly improved the recorded periodontal outcomes between T0 and T1 (FMPS, FMBS, CAL, PPD). Inter-group differences were not statistically significant (p < 0.05). The linear regression models showed that the optimized diet was associated with a higher reduction in PPD and FMBS after the treatment, while patients who had higher LDL levels (over 100 mg/mL) had a less favorable improvement of PPD. The application of an improved diet plan can increase the reduction in PPD and FMBS after non-surgical periodontal therapy when compared with periodontal treatment alone.
Characterizing the Dynamic Evolution of Interagency Collaborative Decision-Making Networks in Response to COVID-19 in China: A Policy Document Analysis
Authors: Cheng Quan , Zheng Shulin , Xiong Zheng , Lin Minwang
Published: March 2022

Collaborative decision-making across multiple government agencies is considered a critical and effective strategy to combat public health crisis; however, we know little about how the collaborative decision-making works and evolves during periods of crisis. To fill this lacuna, this study uncovers the structure and evolving dynamics of the network by employing a policy document analysis. Based on the policy documents, jointly issued by the agencies of Chinese central government in four phases regarding COVID-19 control, we first constructed a co-occurrence matrix of policy-issuing agencies to outline the network structure, then drew a breadth–depth matrix to identify the role evolution of agencies, and lastly built a two-mode network consisting of policy topics and agencies to determine the evolution mechanisms of policy attentions for each agency. It was found that the network structure of interagency collaboration involves three forms: discrete structure in the early phase, subgroup structure in the middle phase, and connected structure in the latter phase. Agencies embedded in the network can be categorized into three types: leading agencies, key agencies, and auxiliary agencies, with their constituent members changed as the pandemic risks are gradually becoming under control. Furthermore, each type has its own primary policy attentions, but shares some common foci in all four phases and shifts attention in the emergency management process. This study contributes to shedding light on the formation of and variations in collaborative networks in health emergencies and provides policy implications for other countries that have struggled against COVID-19.
Challenges in Diagnosis and Clinical Management of COVID-19 in Patient with B-Cell Chronic Lymphocytic Leukemia (CLL): Report of One Case
Authors: Visco Vincenzo , Lippi Maria Enrichetta , Salerno Gerardo , Licata Maria Angela Vittoria A. C. , de Dominicis Chiara , Antolino Giusy , La Verde Giacinto , Santino Iolanda , Simmaco Maurizio , Sciacchitano Salvatore
Published: March 2022

We report here a case of a patient affected by B-cell chronic lymphocytic leukemia (CLL) that developed COVID-19 during the actual SARS-CoV-2 outbreak. The coexistence of CLL and COVID-19 raises many questions regarding the possible increased risk of developing COVID-19 among patients with CLL, the problems in managing therapies for both diseases and, above all, the difficulties in diagnosing COVID-19 in patients affected by CLL. In our patient, an 84-year-old man, the recognition of COVID-19 was delayed because of its atypical clinical presentation and technical problems related to the methods used for the diagnosis. Based on the symptoms and the radiological aspect of the lung, the occurrence of COVID-19 was suspected. Repeated tests on oro/nasopharyngeal swabs gave negative results, causing a delay in the diagnosis. Moreover, different methods used to identify the SARS-CoV-2 antibodies in serum gave conflicting results, and only two tests were able to identify SARS-CoV-2 Abs of the IgG type. During the clinical course of unrecognized COVID-19, our patient developed severe complications and did not receive any specific treatment for the two diseases. Recognition of COVID-19 in patients with CLL is a challenging task and the most accurate methods are necessary to overcome the diagnostic difficulties encountered.
Outcomes of the Pregnancies with Chronic Myeloid Leukemia in the Tyrosine Kinase Inhibitor Era and Literature Review
Authors: Castillo Dan Ran , Park Daniel , Mehta Akhil , Kaur Simmer , Nguyen Anthony , Akhtari Mojtaba
Published: March 2022

Chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasm (MPN) that accounts for 10% of pregnancy-associated leukemias. The Philadelphia chromosome balanced translocation, t (9:22) (q34; q11.2), is the classic mutation seen in CML. The BCR-ABL oncoprotein encoded by this mutation is a constitutively active tyrosine kinase. Tyrosine kinase inhibitor (TKI) therapy is considered a first-line treatment for CML. However, the literature has revealed risks of teratogenicity with TKI therapy during pregnancy. Understanding the risks and benefits of TKI therapy and alternative therapies such as interferon-alpha (IFN-α) will help clinicians and pregnant patients develop a personalized CML treatment plan. This manuscript presents a case series detailing the management of five pregnancies in two pregnant patients with CML and a literature review of CML management in pregnancy.
Agronomic Performance of Sweet Orange Genotypes under the Brazilian Humid Subtropical Climate
Authors: da Silva-de Paula Michele Corpolato Maia , de Carvalho Deived Uilian , da Cruz Maria Aparecida , Longhi Talita Vigo , Tazima Zuleide Hissano , Behlau Franklin , de Carvalho Sérgio Alves , Leite Rui Pereira
Published: March 2022

The diversification of Citrus spp. orchards, for both scion and rootstock genotypes, is essential to prevent outbreaks of insects and diseases, improve yield and fruit quality, and extend harvesting and industrial juice processing. Furthermore, this enables growers to obtain higher off-season profits. Citrus plantings were prohibited in most regions of the state of Paraná in the past due to the spread of citrus canker disease. Therefore, this study aimed to evaluate the agronomic performance of distinct early- and mid-season sweet orange cultivars (C. sinensis (L.) Osbeck) regarding vegetative growth, fruit quality, and yield under the Brazilian humid subtropical climate in order to select new alternatives of sweet orange for the industrial and fresh fruit markets. The experimental orchard was planted in 2012 with 15 sweet orange cultivars (early-maturing: Bahia Cabula, Diva, Cadenera, Marrs, Midsweet, Paulista, Rubi, and Westin; mid-season maturing: Berna Peret, Jaffa, Khalily White, Fukuhara, Seleta do Rio, Seleta Tardia, and Shamouti) grafted on Rangpur lime (C. limonia (L.) Osbeck). The experimental design was randomized blocks with three replicates and five trees per plot, analyzed between each maturation group. Data were submitted to analysis of variance followed by Tukey’s test (p ≤ 0.05). Regarding the early-season cultivars, Diva had the tallest trees with largest canopy diameter and volume, differing from Marrs, which had the smallest trees. Shamouti and Khalily White trees were greatly different from all other mid-season cultivars and produced low fruit load over the evaluated period. The early-season Midsweet scored the highest yield and technological index, similar to the mid-season Berna Peret, producing fruits of high juice quality. These genotypes are more effective under the current situation faced by the citrus industry, as the economic life of orchards has been reduced due huanglongbing (HLB). Altogether, Midsweet and Berna Peret genotypes, previously reported as being less susceptible to citrus canker under the same soil–climate condition, are precocious and exhibit higher agronomic potential to be planted in humid subtropical climates, including Brazil and other similar areas around the world.
Resistance Monitoring for Six Insecticides in Vegetable Field-Collected Populations of Spodoptera litura from China
Authors: Zhang Ziyi , Gao Bingli , Qu Cheng , Gong Jingyu , Li Wenxiang , Luo Chen , Wang Ran
Published: March 2022

The common cutworm, Spodoptera litura (Fabricius), is a notorious and damaging insect pest of horticultural crops in China, the management of which largely relies on chemical agents that are limited by the development of chemical resistance in target populations. As such, resistance monitoring of S. litura populations is a necessary part of management strategies of insecticide resistance. In the current work, we monitored resistance to six insecticides in field-collected populations of S. litura sampled from eleven provinces across China in 2021. The results show that S. litura populations developed significant resistance against chlorantraniliprole, cyantraniliprole, metaflumizone, and pyridalyl and low levels of resistance to chromafenozide. However, S. litura populations were susceptible or exhibited minimal resistance to tetraniliprole. Possible cross-resistances between chlorantraniliprole, cyantraniliprole, metaflumizone, pyridalyl, and chromafenozide were found by pairwise correlation, which also revealed that tetraniliprole lacked cross-resistance with all insecticides tested. Our results suggest suspending the use of chemical agents against which S. litura displayed significant field-evolved resistance, such as chlorantraniliprole, metaflumizone, and pyridalyl, in favor of pesticides against which S. litura was susceptible or exhibited minimal resistance, such as tetraniliprole and chromafenozide, which may help slow the development of insecticide resistance, and in which field management programs aimed at controlling S. litura in China would benefit from the integration of such survey-informed insecticide application strategies. Moreover, the baseline susceptibility confirmed for the six tested insecticides can contribute to design strategies of resistance management for S. litura.
Exogenous Melatonin Protects Lime Plants from Drought Stress-Induced Damage by Maintaining Cell Membrane Structure, Detoxifying ROS and Regulating Antioxidant Systems
Authors: Jafari Marziyeh , Shahsavar Ali Reza , Talebi Majid , Hesami Mohsen
Published: March 2022

Lime is an important commercial product in tropical and subtropical regions, where drought stress is becoming one of the most severe environmental challenges in the agricultural sector. Melatonin is an antioxidant molecule that helps plants regulate their development and respond to a variety of stresses. In this research, the effects of exogenous melatonin treatments were evaluated at different concentrations (0, 50, 100, and 150 μM) on biochemical aspects and gene expression in two species of lime plants (“Mexican lime” and “Persian lime”) under normal (100% field capacity (FC)) and drought stress conditions (75% and 40% FC). The experiments were factorial and based on a completely randomized design (CRD) with four replicates. Drought stress caused electrolyte leakage (EL) as well as accumulations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), indicating the occurrence of damage to cellular membranes. In contrast, the melatonin pretreatment at various concentrations reduced the levels of EL, H2O2 and MDA while mitigating the negative effects of drought stress on the two lime species. The application of melatonin (100-μM) significantly increased the level of proline content and activity of antioxidant enzymes in plants under drought stress compared to control plants. According to real-time PCR analysis, drought stress and melatonin treatment enhanced the expression of genes involved in ROS scavenging, proline biosynthesis, and cell redox regulation in both species, as compared to their respective controls. According to these findings, melatonin is able to detoxify ROS and regulate antioxidant systems, thereby protecting lime plants from drought stress-induced damages.
The Bioactive Compounds and Fatty Acid Profile of Bitter Apple Seed Oil Obtained in Hot, Arid Environments
Authors: Berwal Mukesh Kumar , Ram Chet , Gurjar Pawan Singh , Gora Jagan Singh , Kumar Ramesh , Verma Ajay Kumar , Singh Dhurendra , Basile Boris , Rouphael Youssef , Kumar Pradeep
Published: March 2022

Bitter apple or tumba (Citrullus colocynthis L.) is a prostrate annual herb belonging to the Cucurbitaceae family. It is highly tolerant against multiple abiotic stresses like drought, heat, and soil salinity and can easily grow on very marginal soil, even on sand dunes in hot, arid regions. Tumba fruit is a fleshy berry 5–10 cm in diameter and of a pale yellow color at ripening. The tumba fruit used in this research was harvested from the ICAR-CIAH, Bikaner research farm. The seeds were separated, and their oil was extracted to analyze its physical characteristics and composition (phytochemical compounds, fatty acid profile, etc.). The seeds of the tumba fruit contained 23–25% golden-yellow-colored oil with a specific gravity of 0.92 g/mL. The extracted oil contained appreciable amounts of phytochemical (bioactive) compounds like phenolics (5.39 mg GAE/100 g), flavonoids (938 mg catechin eq./100 g), carotenoids (79.5 mg/kg), oryzanol (0.066%), and lignans (0.012%), along with 70–122 mg AAE/100 g total antioxidant activity (depending on the determination method). The results of fatty acid profiling carried out by GC-MS/MS demonstrated that tumba seed oil contained about 70% unsaturated fatty acids with more than 51% polyunsaturated fatty acids. It mainly contained linoleic acid (C18:2n6; 50.3%), followed by oleic acid (C18:1n9; 18.0%), stearic acid (C18:0; 15.2%), and palmitic acid (C16:0; 12.4%). Therefore, this oil can be considered as a very good source of essential fatty acids like omega-6 fatty acid (linoleic acid), whereas it contains a lower concentration of omega-3 fatty acids (α-linolenic acid) and hydroxy polyunsaturated fatty acids. In addition, it also contains some odd chain fatty acids like pentadecanoic and heptadecanoic acid (C15:0 and C17:0, respectively), which have recently been demonstrated to be bioactive compounds in reducing the risk of cardiometabolic diseases. The results of this study suggest that tumba seed oil contains several health-promoting bioactive compounds with nutraceutical properties; hence, it can be an excellent dietary source.