bluuebunny/crossref_metadata_2025 huggingface dataset:
The Measurement of Goal Orientation in Sport: Psychometric Properties of the Polish Version of the Perception of Success Questionnaire (POSQ)
Authors: Tomczak Maciej , Walczak Małgorzata , Kleka Paweł , Walczak Aleksandra , Bojkowski Łukasz
Published: September 2020

The main aim of the study is a comprehensive assessment of psychometric properties of the Polish version of Perception of Success Questionnaire (POSQ) in sport. Apart from standard psychometric evaluation, the paper presents an analysis of item reliability through the use of Item Response Theory, as well as the analysis of relationships between sport type, level of participation, gender and goal orientation level. The study covered 412 people aged M = 23.46 (SD = 5.40). The Perception of Success Questionnaire (POSQ), the Task and Ego Orientation in Sport Questionnaire (TEOSQ) and the Sport Motivation Scale (SMS-28) were used. High reliability of POSQ ego subscale (α = 0.89, ω = 0.89) and POSQ task subscale (α = 0.90, ω = 0.91) were noted. The test-retest correlations at the two-week interval were ICC = 0.91 for ego subscale, and ICC = 0.71 for task subscale, respectively. Confirmatory factor analysis showed a relatively good fit of the two-factor model to the data (CFI = 0.94). Relationships between the goal orientation measured by the POSQ questionnaire and motivational traits measured by TEOSQ and SMS-28 were obtained. It was also shown that high-performance athletes had higher scores on the ego factor than recreational athletes. Moreover, men had higher scores on the ego factor than women. The Perception of Success Questionnaire (Polish version) is characterized by satisfactory psychometric properties and can be used for scientific research and diagnosis.
Uncertainties, Fear and Stigma: Perceptions of Zika Virus among Pregnant Women in Spain
Authors: Marbán-Castro Elena , Villén-Gonzalvo Ana , Enguita-Fernàndez Cristina , Marín-Cos Anna , Menéndez Clara , Maixenchs Maria , Bardají Azucena
Published: September 2020

Similar to other epidemics, knowledge about Zika virus (ZIKV) relies upon information often coming from outside the health system. This study aimed to explore views, perceptions and attitudes towards ZIKV among migrant women from Central and South America, diagnosed with ZIKV infection during pregnancy, and to comprehend healthcare professionals’ perceptions of ZIKV. An exploratory qualitative study, based on phenomenology and grounded theory, was conducted in Barcelona, Spain. Data were collected through in-depth and paired interviews with women diagnosed with ZIKV infection during pregnancy, and semi-structured interviews with healthcare professionals. Women showed good level of awareness of ZIKV, despite some knowledge gaps. The most consulted source of information about ZIKV was the Internet. Women expressed they suffered from anxiety and depression due to potential effects of ZIKV on their babies. They conveyed their sources of support came primarily from their partners and relatives, as well as healthcare professionals. This study stresses the dramatic health, social and emotional burden that the epidemic imposed on migrant women infected with ZIKV during pregnancy. These results may help guide psychosocial support and health measures for pregnant women and their children as part of the public health emergency response in emergent epidemics.
Thermogenic Activation Downregulates High Mitophagy Rate in Human Masked and Mature Beige Adipocytes
Authors: Szatmári-Tóth Mária , Shaw Abhirup , Csomós István , Mocsár Gábor , Fischer-Posovszky Pamela , Wabitsch Martin , Balajthy Zoltán , Lányi Cecília , Győry Ferenc , Kristóf Endre , Fésüs László
Published: September 2020

Thermogenic brown and beige adipocytes oxidize metabolic substrates producing heat, mainly by the mitochondrial uncoupling protein UCP1, and can thus counteract obesity. Masked beige adipocytes possess white adipocyte-like morphology, but can be made thermogenic by adrenergic stimuli. We investigated the regulation of mitophagy upon thermogenic activation of human masked and mature beige adipocytes. Human primary abdominal subcutaneous adipose-derived stromal cells (hASCs) and Simpson–Golabi–Behmel syndrome (SGBS) preadipocytes were differentiated to white and beige adipocytes, then their cAMP-induced thermogenic potential was assessed by detecting increased expressions of UCP1, mitochondrial DNA content and respiratory chain complex subunits. cAMP increased the thermogenic potential of white adipocytes similarly to beige ones, indicating the presence of a masked beige population. In unstimulated conditions, a high autophagic flux and mitophagy rates (demonstrated by LC3 punctae and TOM20 co-immunostaining) were observed in white adipocytes, while these were lower in beige adipocytes. Silencing and gene expression experiments showed that the ongoing mitophagy was Parkin-independent. cAMP treatment led to the downregulation of mitophagy through PKA in both types of adipocytes, resulting in more fragmented mitochondria and increased UCP1 levels. Our data indicates that mitophagy is repressed upon encountering a short-term adrenergic stimulus, as a fast regulatory mechanism to provide high mitochondrial content for thermogenesis.
Effect of Urban Particulate Matter on Vocal Fold Fibrosis through the MAPK/NF-κB Signaling Pathway
Authors: Won Ho-Ryun , Jung Seung-Nam , Yeo Min-Kyung , Yi Shinae , Liu Lihua , Lim Mi Ae , Oh Chan , Kang Yea Eun , Chang Jae Won , Rha Ki Sang , Koo Bon Seok
Published: September 2020

Particulate matter (PM) is an environmental exposure factor that adversely affects human health. PM is a risk factor for various diseases. However, the mechanism by which PM affects the vocal folds (VF) has not yet been evaluated. Thus, we investigated the cytotoxic effects of PM on human vocal fold fibroblasts (hVFF) and the underlying signaling pathways. hVFF were isolated from human VF. The effect of PM on hVFF, and the underlying mechanism, were analyzed using Western blot, quantitative real-time polymerase chain reaction, and flow cytometry. In addition, a histological evaluation was performed in animal experiments. Cell proliferation decreased after the PM treatment. PM increased the expression of interleukin (IL)-6 and IL-1β. The generation of reactive oxygen species (ROS) in PM-treated hVFF and subsequent activation of the mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways were confirmed. Furthermore, PM increased the expression of fibrosis-related markers and induced the accumulation of collagen in the extracellular matrix. As a result, PM exposure significantly enhances the inflammatory response on VF through the ROS-mediated activation of the MAPK and NF-κB signaling pathways. In addition, PM promotes differentiation into myofibroblasts and induces fibrosis. These results suggest that PM triggers an inflammatory reaction through ROS production and causes VF fibrosis.
AAA+ Molecular Chaperone ClpB in Leptospira interrogans: Its Role and Significance in Leptospiral Virulence and Pathogenesis of Leptospirosis
Authors: Kędzierska-Mieszkowska Sabina , Arent Zbigniew
Published: September 2020

Bacterial ClpB is an ATP-dependent disaggregase that belongs to the Hsp100/Clp subfamily of the AAA+ ATPases and cooperates with the DnaK chaperone system in the reactivation of aggregated proteins, as well as promotes bacterial survival under adverse environmental conditions, including thermal and oxidative stresses. In addition, extensive evidence indicates that ClpB supports the virulence of numerous bacteria, including pathogenic spirochaete Leptospira interrogans responsible for leptospirosis in animals and humans. However, the specific function of ClpB in leptospiral virulence still remains to be fully elucidated. Interestingly, ClpB was predicted as one of the L. interrogans hub proteins interacting with human proteins, and pathogen–host protein interactions are fundamental for successful invasion of the host immune system by bacteria. The aim of this review is to discuss the most important aspects of ClpB’s function in L. interrogans, including contribution of ClpB to leptospiral virulence and pathogenesis of leptospirosis, a zoonotic disease with a significant impact on public health worldwide.
Milk Exosomes: Perspective Agents for Anticancer Drug Delivery
Authors: Sedykh Sergey , Kuleshova Anna , Nevinsky Georgy
Published: September 2020

Exosomes are biological nanovesicles that participate in intercellular communication by transferring biologically active chemical compounds (proteins, microRNA, mRNA, DNA, and others). Due to their small size (diameter 40–100 nm) and high biological compatibility, exosomes are promising delivery tools in personalized therapy. Because artificial exosome synthesis methods are not developed yet, the urgent task is to develop an effective and safe way to obtain exosomes from natural sources. Milk is the only exosome-containing biological fluid that is commercially available. In this regard, milk exosomes are unique and promising candidates for new therapeutic approaches to treating various diseases, including cancer. The appearance of side effects during the use of cytotoxic and cytostatic agents is among the main problems in cancer chemotherapy. According to this, the targeted delivery of chemotherapeutic agents can be a potential solution to the toxic effect of chemotherapy. The ability of milk exosomes to carry out biologically active substances to the cell makes them promising tools for oral delivery of chemotherapeutic agents. This review is devoted to the methods of milk exosome isolation, their biological components, and prospects for their use in cancer treatment.
Ionizing Radiation and Translation Control: A Link to Radiation Hormesis?
Authors: Kabilan Usha , Graber Tyson E. , Alain Tommy , Klokov Dmitry
Published: September 2020

Protein synthesis, or mRNA translation, is one of the most energy-consuming functions in cells. Translation of mRNA into proteins is thus highly regulated by and integrated with upstream and downstream signaling pathways, dependent on various transacting proteins and cis-acting elements within the substrate mRNAs. Under conditions of stress, such as exposure to ionizing radiation, regulatory mechanisms reprogram protein synthesis to translate mRNAs encoding proteins that ensure proper cellular responses. Interestingly, beneficial responses to low-dose radiation exposure, known as radiation hormesis, have been described in several models, but the molecular mechanisms behind this phenomenon are largely unknown. In this review, we explore how differences in cellular responses to high- vs. low-dose ionizing radiation are realized through the modulation of molecular pathways with a particular emphasis on the regulation of mRNA translation control.
Editorial: Special Issue on “Therapeutic Approaches for Cystic Fibrosis”
Authors: Pedemonte Nicoletta
Published: September 2020

Cystic fibrosis (CF) is the most common lethal genetic disease in Caucasian populations, occurring in approximately 1 in 3000 newborns worldwide [...]
A Novel Role of Interleukin-6 as a Regulatory Factor of Inflammation-Associated Deterioration in Osteoblast Arrangement
Authors: Matsugaki Aira , Matsumoto Shun , Nakano Takayoshi
Published: September 2020

Inflammatory disorders are associated with bone destruction; that is, deterioration in bone cell activities are under the control of the innate immune system. Macrophages play a central role in innate immunity by switching their polarized phenotype. A disturbed immune system causes aberrance in the ordered bone matrix microarrangement, which is a dominant determinant of bone tissue functionalization. However, the precise relationship between the immune system and bone tissue organization is unknown. In this study, the controlled in vitro co-culture assay results showed that M1-polarized macrophages disrupted the osteoblast alignment, which directly modulate the oriented bone matrix organization, by secreting pro-inflammatory cytokines. Notably, interleukin-6 was found to be a key regulator of unidirectional osteoblast alignment. Our results demonstrated that inflammatory diseases triggered bone dysfunction by regulating the molecular interaction between the immune system and bone tissue organization. These findings may contribute to the development of therapeutic targets for inflammatory disorders, including rheumatoid arthritis.
Ameliorative Properties of Boronic Compounds in In Vitro and In Vivo Models of Alzheimer’s Disease
Authors: Maiti Panchanan , Manna Jayeeta , Burch Zoe N. , Flaherty Denise B. , Larkin Joseph D. , Dunbar Gary L.
Published: September 2020

Alzheimer’s disease (AD) is characterized by amyloid (Aβ) aggregation, hyperphosphorylated tau, neuroinflammation, and severe memory deficits. Reports that certain boronic compounds can reduce amyloid accumulation and neuroinflammation prompted us to compare trans-2-phenyl-vinyl-boronic-acid-MIDA-ester (TPVA) and trans-beta-styryl-boronic-acid (TBSA) as treatments of deficits in in vitro and in vivo models of AD. We hypothesized that these compounds would reduce neuropathological deficits in cell-culture and animal models of AD. Using a dot-blot assay and cultured N2a cells, we observed that TBSA inhibited Aβ42 aggregation and increased cell survival more effectively than did TPVA. These TBSA-induced benefits were extended to C. elegans expressing Aβ42 and to the 5xFAD mouse model of AD. Oral administration of 0.5 mg/kg dose of TBSA or an equivalent amount of methylcellulose vehicle to groups of six- and 12-month-old 5xFAD or wild-type mice over a two-month period prevented recognition- and spatial-memory deficits in the novel-object recognition and Morris-water-maze memory tasks, respectively, and reduced the number of pyknotic and degenerated cells, Aβ plaques, and GFAP and Iba-1 immunoreactivity in the hippocampus and cortex of these mice. These findings indicate that TBSA exerts neuroprotective properties by decreasing amyloid plaque burden and neuroinflammation, thereby preventing neuronal death and preserving memory function in the 5xFAD mice.
Evaluating Richer Features and Varied Machine Learning Models for Subjectivity Classification of Book Review Sentences in Portuguese
Authors: Belisário Luana Balador , Ferreira Luiz Gabriel , Pardo Thiago Alexandre Salgueiro
Published: September 2020

Texts published on social media have been a valuable source of information for companies and users, as the analysis of this data helps improving/selecting products and services of interest. Due to the huge amount of data, techniques for automatically analyzing user opinions are necessary. The research field that investigates these techniques is called sentiment analysis. This paper focuses specifically on the task of subjectivity classification, which aims to predict whether a text passage conveys an opinion. We report the study and comparison of machine learning methods of different paradigms to perform subjectivity classification of book review sentences in Portuguese, which have shown to be a challenging domain in the area. Specifically, we explore richer features for the task, using several lexical, centrality-based and discourse features. We show the contributions of the different feature sets and evidence that the combination of lexical, centrality-based and discourse features produce better results than any of the feature sets individually. Additionally, by analyzing the achieved results and the acquired knowledge by some symbolic machine learning methods, we show that some discourse relations may clearly signal subjectivity. Our corpus annotation also reveals some distinctive discourse structuring patterns for sentence subjectivity.
Tracking Red Palm Mite Damage in the Western Hemisphere Invasion with Landsat Remote Sensing Data
Authors: Rodrigues Jose Carlos Verle , Cosh Michael H. , Hunt E. Raymond , de Moraes Gilberto J. , Barroso Geovanny , White William A. , Ochoa Ronald
Published: September 2020

Red palm mites (Raoiella indica Hirst, Acari: Tenuipalpidae) were first observed in the western hemisphere on the islands and countries surrounding the Caribbean Sea, infesting the coconut palm (Cocos nucifera L.). Detection of invasive pests usually relies upon changes in vegetation properties as result of the pest activity. These changes may be visible in time series of satellite data records, such as Landsat satellites, which have been available with a 16-day repeat cycle at a spatial resolution of 30 m since 1982. Typical red palm mite infestations result in the yellowing of the lower leaves of the palm crown; remote sensing model simulations have indicated that this feature may be better detected using the green normalized difference vegetation index (GNDVI). Using the Google Earth Engine programming environment, a time series of Landsat 5 Thematic Mapper, Landsat 7 Enhanced Thematic Mapper Plus and Landsat 8 Operational Land Imager data was generated for plantations in northern and northeast Brazil, El Salvador, and Trinidad-Tobago. Considering the available studied plantations, there were little or no differences of GNDVI before and after the dates when red palm mites were first revealed at each location. A discussion of possible alternative approaches are discussed related to the limitations of the current satellite platforms.
Is There a Role for Spacer Exchange in Two-Stage Exchange Arthroplasty for Periprosthetic Joint Infection?
Authors: Kozaily Elie , Chisari Emanuele , Parvizi Javad
Published: September 2020

Periprosthetic joint infection (PJI) continues to be one of the most serious complications after hip and knee arthroplasty. The choice of surgical treatment depends on a multitude of factors like chronicity of infection, host factors, and institutional or surgeon experience. Two-stage exchange remains one of the most commonly used technique for chronic PJI in the United States of America. The intended two-stage revision may involve an additional interim procedure where the initial antibiotic cement spacer is removed and a new spacer is inserted. Mostly, the rationale behind spacer exchange is an additional load of local antibiotics before proceeding to reimplantation. There is no conclusive evidence whether a spacer exchange confers additional benefits, yet it delays reimplantation and exposes already fragile patients to the risks and morbidity of an additional surgery.
Xeno-Free Condition Enhances Therapeutic Functions of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells against Experimental Colitis by Upregulated Indoleamine 2,3-Dioxygenase Activity
Authors: Kang Ji Yeon , Oh Mi-Kyung , Joo Hansol , Park Hyun Sung , Chae Dong-Hoon , Kim Jieun , Lee Hae-Ri , Oh Il-Hoan , Yu Kyung-Rok
Published: September 2020

The therapeutic applications of mesenchymal stem cells (MSCs) have been actively explored due to their broad anti-inflammatory and immunomodulatory properties. However, the use of xenogeneic components, including fetal bovine serum (FBS), in the expansion media might pose a risk of xenoimmunization and zoonotic transmission to post-transplanted patients. Here, we extensively compared the physiological functions of human Wharton’s jelly-derived MSCs (WJ-MSCs) in a xeno-free medium (XF-MSCs) and a medium containing 10% FBS (10%-MSCs). Both groups showed similar proliferation potential; however, the 10%-MSCs showed prolonged expression of CD146, with higher colony-forming unit-fibroblast (CFU-F) ability than the XF-MSCs. The XF-MSCs showed enhanced adipogenic differentiation potential and sufficient hematopoietic stem cell (HSC) niche activity, with elevated niche-related markers including CXCL12. Furthermore, we demonstrated that the XF-MSCs had a significantly higher suppressive effect on human peripheral blood-derived T cell proliferation, Th1 and Th17 differentiation, as well as naïve macrophage polarization toward an M1 phenotype. Among the anti-inflammatory molecules, the production of indoleamine 2,3-dioxygenase (IDO) and nitric oxide synthase 2 (NOS2) was profoundly increased, whereas cyclooxygenase-2 (COX-2) was decreased in the XF-MSCs. Finally, the XF-MSCs had an enhanced therapeutic effect against mouse experimental colitis. These findings indicate that xeno-free culture conditions improved the immunomodulatory properties of WJ-MSCs and ex vivo-expanded XF-MSCs might be an effective strategy for preventing the progression of colitis.
Comparing the Diagnostic Value of Serum D-Dimer to CRP and IL-6 in the Diagnosis of Chronic Prosthetic Joint Infection
Authors: Ackmann Thomas , Möllenbeck Burkhard , Gosheger Georg , Schwarze Jan , Schmidt-Braekling Tom , Schneider Kristian Nikolaus , Frommer Adrien , Dieckmann Ralf , Theil Christoph
Published: September 2020

Introduction: D-dimer is a diagnostic criterion for periprosthetic joint infection (PJI) of the Musculoskeletal Infection Society (MSIS) in 2018. The aim of this study was to evaluate the serum D-dimer values in comparison to C-reactive protein (CRP) and interleukin-6 (IL-6) for the diagnosis of PJI. Materials and Methods: We included 119 patients (50 women, 69 men; 71 knees, 48 hips) undergoing revision arthroplasty with preoperative assessment of CRP, IL-6, and serum D-dimer. Cases were classified as infected or aseptic based on the MSIS criteria of 2018. Receiver operating curves and Youden’s index were used to define an ideal cut-off value and sensitivity and specificity for the individual parameters, and respective combinations were calculated using cross-tables. Results: The median D-dimer level (2320 vs. 1105 ng/mL; p < 0.001), the median CRP level (4.0 vs. 0.5 mg/dL; p < 0.001), and the median IL-6 level (21.0 vs. 5.0 pg/mL; p < 0.001) were significantly higher in the group of PJI compared to the group with aseptic failure. The calculated optimal cut-off values were 2750 ng/mL (AUC 0.767) for D-dimer, 1.2 mg/dL (AUC 0.914) for CRP, and 10.0 pg/mL (AUC 0.849) for IL-6. D-dimer showed a sensitivity of 38% and specificity of 94%, whereas the CRP and IL-6 had sensitivities of 88% and 76%, and specificities of 87% and 92%, respectively. Conclusion: In comparison with CRP and IL-6, serum D-dimer showed low sensitivity and specificity in our cohort. While CRP and IL-6 combination had the highest sensitivity, a combination of Il-6 and D-dimer or CRP and IL-6 had the highest specificity.
Early Post-Rewarming Fever Is Associated with Favorable 6-Month Neurologic Outcomes in Patients with Out-Of-Hospital Cardiac Arrest: A Multicenter Registry Study
Authors: Lee Hyoung Youn , Lee Dong Hun , Lee Byung Kook , Jeung Kyung Woon , Jung Yong Hun , Choi Seung Phil , Park Jung Soo , Lee Jae Hoon , Han Kap Su , Min Yong Il
Published: September 2020

We investigated the association between post-rewarming fever (PRF) and 6-month neurologic outcomes in cardiac arrest survivors. This was a multicenter study based on a registry of comatose adult (≥18 years) out-of-hospital cardiac arrest (OHCA) survivors who underwent targeted temperature management between October 2015 to December 2018. PRF was defined as peak temperature ≥ 38.0 °C within 72 h after completion of rewarming, and PRF timing was categorized as within 24, 24–48, and 48–72 h epochs. The primary outcome was neurologic outcomes at six months after cardiac arrest. Unfavorable neurologic outcome was defined as cerebral performance categories three to five. A total of 1031 patients were included, and 642 (62.3%) had unfavorable neurologic outcomes. PRF developed in 389 (37.7%) patients in 72 h after rewarming: within 24 h in 150 (38.6%), in 24–48 h in 155 (39.8%), and in 48–72 h in 84 (21.6%). PRF was associated with improved neurologic outcomes (odds ratio (OR), 0.633; 95% confidence interval (CI), 0.416–0.963). PRF within 24 h (OR, 0.355; 95% CI, 0.191–0.659), but not in 24–48 h or 48–72 h, was associated with unfavorable neurologic outcomes. Early PRF within 24 h after rewarming was associated with favorable neurologic outcomes.
Accuracy of Physical Examination to Detect Synovial and Extra-Synovial Pathologies in Psoriatic Arthritis in Comparison to Ultrasonography
Authors: Gazel Ummugulsum , Solmaz Dilek , Ayan Gizem , Ivory Catherine , Karsh Jacob , Aydin Sibel Zehra
Published: September 2020

We aimed to explore the accuracy of physical examination (PE) to detect the synovial and extra-synovial pathologies in psoriatic arthritis (PsA) in comparison to ultrasonography (US). Twenty-nine PsA patients with hand pain were included in the study. A detailed PE of the hands was performed and US scans were performed for the joints, extensor and flexor tendons, and entheses of the second to fifth fingers of both hands. The agreement between PE and US findings was calculated. The strongest agreement for the joints was between “swollen joints” and power Doppler (PD) signals in the metacarpophalangeal (MCP) joints and grey scale synovitis in the proximal interphalangeal (PIP) joints. The agreement of tender entheses on PE and inflammation on US (hypoechogenicity, thickening, and/or PD signals) was poor for both extensor (Kappa = −0.027, Prevalence Adjusted and Bias Adjusted Kappa (PABAK) = 0.344) and flexor compartments (Kappa = 0.039, PABAK = 0.569). Similar to enthesitis, comparison of any PE and US findings showed a poor agreement at the extensor and flexor tendon regions (extensor: Kappa = 0.123, PABAK = 0.448, and flexor: Kappa = 0.171, PABAK = 0.431). Our study showed that there was a poor to fair agreement of PE and US findings of hands. US can add value when determining the source of pain in PsA in the small joints.
Neutrophil Extracellular Traps and By-Products Play a Key Role in COVID-19: Pathogenesis, Risk Factors, and Therapy
Authors: Thierry Alain R. , Roch Benoit
Published: September 2020

Understanding of the pathogenesis of the coronavirus disease-2019 (COVID-19) remains incomplete, particularly in respect to the multi-organ dysfunction it may cause. We were the first to report the analogous biological and physiological features of COVID-19 pathogenesis and the harmful amplification loop between inflammation and tissue damage induced by the dysregulation of neutrophil extracellular traps (NETs) formation. Given the rapid evolution of this disease, the nature of its symptoms, and its potential lethality, we hypothesize that COVID-19 progresses under just such an amplifier loop, leading to a massive, uncontrolled inflammation process. Here, we describe in-depth the correlations of COVID-19 symptoms and biological features with those where uncontrolled NET formation is implicated in various sterile or infectious diseases. General clinical conditions, as well as numerous pathological and biological features, are analogous with NETs deleterious effects. Among NETs by-products implicated in COVID-19 pathogenesis, one of the most significant appears to be elastase, in accelerating virus entry and inducing hypertension, thrombosis and vasculitis. We postulate that severe acute respiratory syndrome-coronavirus 2 (SARS-CoV2) may evade innate immune response, causing uncontrolled NETs formation and multi-organ failure. In addition, we point to indicators that NETS-associated diseases are COVID-19 risk factors. Acknowledging that neutrophils are the principal origin of extracellular and circulating DNA release, we nonetheless, explain why targeting NETs rather than neutrophils themselves may in practice be a better strategy. This paper also offers an in-depth review of NET formation, function and pathogenic dysregulation, as well as of current and prospective future therapies to control NETopathies. As such, it enables us also to suggest new therapeutic strategies to fight COVID-19. In combination with or independent of the latest tested approaches, we propose the evaluation, in the short term, of treatments with DNase-1, with the anti-diabetic Metformin, or with drugs targeting elastase (i.e., Silvelestat). With a longer perspective, we also advocate a significant increase in research on the development of toll-like receptors (TLR) and C-type lectin-like receptors (CLEC) inhibitors, NET-inhibitory peptides, and on anti-IL-26 therapies.
body2vec: 3D Point Cloud Reconstruction for Precise Anthropometry with Handheld Devices
Authors: Trujillo-Jiménez Magda Alexandra , Navarro Pablo , Pazos Bruno , Morales Leonardo , Ramallo Virginia , Paschetta Carolina , De Azevedo Soledad , Ruderman Anahí , Pérez Orlando , Delrieux Claudio , Gonzalez-José Rolando
Published: September 2020

Current point cloud extraction methods based on photogrammetry generate large amounts of spurious detections that hamper useful 3D mesh reconstructions or, even worse, the possibility of adequate measurements. Moreover, noise removal methods for point clouds are complex, slow and incapable to cope with semantic noise. In this work, we present body2vec, a model-based body segmentation tool that uses a specifically trained Neural Network architecture. Body2vec is capable to perform human body point cloud reconstruction from videos taken on hand-held devices (smartphones or tablets), achieving high quality anthropometric measurements. The main contribution of the proposed workflow is to perform a background removal step, thus avoiding the spurious points generation that is usual in photogrammetric reconstruction. A group of 60 persons were taped with a smartphone, and the corresponding point clouds were obtained automatically with standard photogrammetric methods. We used as a 3D silver standard the clean meshes obtained at the same time with LiDAR sensors post-processed and noise-filtered by expert anthropological biologists. Finally, we used as gold standard anthropometric measurements of the waist and hip of the same people, taken by expert anthropometrists. Applying our method to the raw videos significantly enhanced the quality of the results of the point cloud as compared with the LiDAR-based mesh, and of the anthropometric measurements as compared with the actual hip and waist perimeter measured by the anthropometrists. In both contexts, the resulting quality of body2vec is equivalent to the LiDAR reconstruction.
Combining Simulation and Machine Learning as Digital Twin for the Manufacturing of Overmolded Thermoplastic Composites
Authors: Hürkamp André , Gellrich Sebastian , Ossowski Tim , Beuscher Jan , Thiede Sebastian , Herrmann Christoph , Dröder Klaus
Published: September 2020

The design and development of composite structures requires precise and robust manufacturing processes. Composite materials such as fiber reinforced thermoplastics (FRTP) provide a good balance between manufacturing time, mechanical performance and weight. In this contribution, we investigate the process combination of thermoforming FRTP sheets (organo sheets) and injection overmolding of short FRTP for automotive structures. The limiting factor in those structures is the bond strength between the organo sheet and the overmolded thermoplastic. Within this process chain, even small deviations of the process settings (e.g., temperature) can lead to significant defects in the structure. A cyber physical production system based framework for a digital twin combining simulation and machine learning is presented. Based on parametric Finite-Element-Method (FEM) studies, training data for machine learning methods are generated and a FEM surrogate is developed. A comparison of different data-driven methods yields information on the estimation accuracy of task-specific data-driven methods. Finally, in accordance with experimental cross tension tests, the investigated FEM surrogate model is able to predict the interface bond strength quality in dependence of the process settings. The visualization into different quality domains qualifies the presented approach as decision support.
Applying Two Active Acoustic Technologies to Document Presence of Large Marine Animal Targets at a Marine Renewable Energy Site
Authors: Staines Garrett , Zydlewski Gayle Barbin , Viehman Haley A. , Kocik Rachel
Published: September 2020

Coastal regions are highly used by humans. The growing marine renewable energy (MRE) industry will add to existing anthropogenic pressures in these regions. Regulatory bodies require animal risk assessment before new industrial activities can progress, and MRE is no exception. Preliminary data of marine mammal use of an MRE device deployment location could be informative to permitting. A combination of downlooking hydroacoustics using an echosounder and acoustic camera (imaging sonar) was used to provide a number of large targets (proxy for large fish and marine mammals) in an area of interest for MRE tidal turbine deployment in Western Passage, Maine, USA. Data were collected in May, June, August, and September of 2010 and 2011. Of the nine large targets confirmed to be animals, eight were porpoises and one was a shark. Few large targets were observed in May and June, with the majority (90%) being present in August and September of both years. The most large targets were observed when tidal current speed was less than 1 m·s−1. These data provide a preliminary assessment of large targets in a single location over sixteen 24-h surveys. The aforementioned methodology could be used for future pre- and post-installation assessments at MRE device deployment locations. Their use in concert with visual and passive acoustic monitoring can provide water depth usage by marine mammals, which is a metric that is difficult to assess with passive acoustic and visual techniques.
Interplay between Fungal Infection and Bacterial Associates in the Wax Moth Galleria mellonella under Different Temperature Conditions
Authors: Kryukov Vadim Yu , Kosman Elena , Tomilova Oksana , Polenogova Olga , Rotskaya Ulyana , Tyurin Maksim , Alikina Tatyana , Yaroslavtseva Olga , Kabilov Marsel , Glupov Viktor
Published: September 2020

Various insect bacterial associates are involved in pathogeneses caused by entomopathogenic fungi. The outcome of infection (fungal growth or decomposition) may depend on environmental factors such as temperature. The aim of this study was to analyze the bacterial communities and immune response of Galleria mellonella larvae injected with Cordyceps militaris and incubated at 15 °C and 25 °C. We examined changes in the bacterial CFUs, bacterial communities (Illumina MiSeq 16S rRNA gene sequencing) and expression of immune, apoptosis, ROS and stress-related genes (qPCR) in larval tissues in response to fungal infection at the mentioned temperatures. Increased survival of larvae after C. militaris injection was observed at 25 °C, although more frequent episodes of spontaneous bacteriosis were observed at this temperature compared to 15 °C. We revealed an increase in the abundance of enterococci and enterobacteria in the midgut and hemolymph in response to infection at 25 °C, which was not observed at 15 °C. Antifungal peptide genes showed the highest expression at 25 °C, while antibacterial peptides and inhibitor of apoptosis genes were strongly expressed at 15 °C. Cultivable bacteria significantly suppressed the growth of C. militaris. We suggest that fungi such as C. militaris may need low temperatures to avoid competition with host bacterial associates.
The Effect of Nickel on the Microstructure, Mechanical Properties and Corrosion Properties of Niobium–Vanadium Microalloyed Powder Metallurgy Steels
Authors: Ahssi Mohamed Ahmed Mohamed , Erden Mehmet Akif , Acarer Mustafa , Çuğ Harun
Published: September 2020

In this study, the effects of adding Ni in different ratios to Fe-matrix material containing C-Nb-V produced by powder metallurgy on microstructure, tensile strength, hardness and corrosion behaviors were investigated. Fe-C and Fe-C-Nb-V powders containing 5%, 10%, 13%, 15%, 20%, 30% and 40% nickel were pressed at 700 MPa and then sintered in an Ar atmosphere at 1400 °C. Microstructures of the samples were characterized with optical microscope, scanning electron microscope (SEM) and XRD. Corrosion behaviors were investigated by obtaining Tafel curves in an aqueous solution containing 3.5% NaCl. Mechanical properties were determined by hardness and tensile testing. While Fe-C alloy and Fe-C-Nb-V microalloyed steel without Ni typically have a ferrite-pearlite microstructure, the austenite phase has been observed in the microstructures of the alloys with 10% nickel and further. Yield and tensile strength increased with nickel content and reached the highest strength values with 13% Ni content. The addition of more nickel led to decrease the strength. Analysis of Tafel curves showed that corrosion resistance of alloys increased with increasing nickel concentration.
Implementation of Recycling Cigarette Butts in Lightweight Bricks and a Proposal for Ending the Littering of Cigarette Butts in Our Cities
Authors: Mohajerani Abbas , Qun Hui Siu , Shen Cary , Suntovski James , Rodwell Glen , Kurmus Halenur , Hana Marven , Rahman Md Tareq
Published: September 2020

Our cities, parks, beaches, and oceans have been contaminated for many years with millions of tonnes of unsightly and toxic cigarette butts (CBs). This study presents and discusses some of the results of an ongoing study on recycling in fired-clay bricks. Energy savings: the energy value of CBs with remnant tobacco was found to be 16.5 MJ/kg. If just 2.5% of all bricks produced annually worldwide included 1% CB content, all of the CBs currently produced could be recycled in bricks, and it is estimated that global firing energy consumption could be reduced by approximately 20 billion MJ (megajoules). This approximately equates to the power used by one million homes in Victoria, Australia, every year. Bacteriological study: CBs were investigated for the presence of ten common bacteria in two pilot studies. Staphylococcus spp. and Pseudomonas aeruginosa were detected in fresh used CB samples, and Listeria spp. were detected in old used CB samples. All of the CB samples except the dried sample had significant counts of Bacillus spp. Some species of the detected bacteria in this study are pathogenic. Further confirmation and comprehensive microbiological study are needed in this area. The contact of naphthalene balls with CBs had a significant disinfecting effect on Bacillus spp. The implementation procedure for recycling CBs in bricks, odour from Volatile Organic Compound (VOC) emissions in CBs, sterilization methods, CB collection systems, and safety instructions were investigated, and they are discussed. Proposal: when considering the combined risks from many highly toxic chemicals and possible pathogens in cigarette butts, it is proposed that littering of this waste anywhere in cities and the environment be strictly prohibited and that offenders be heavily fined.
Solid-State Li-Ion Batteries Operating at Room Temperature Using New Borohydride Argyrodite Electrolytes
Authors: Dao Anh Ha , López-Aranguren Pedro , Zhang Junxian , Cuevas Fermín , Latroche Michel
Published: September 2020

Using a new class of (BH4)− substituted argyrodite Li6PS5Z0.83(BH4)0.17, (Z = Cl, I) solid electrolyte, Li-metal solid-state batteries operating at room temperature have been developed. The cells were made by combining the modified argyrodite with an In-Li anode and two types of cathode: an oxide, LixMO2 (M = ⅓ Ni, ⅓ Mn, ⅓ Co; so called NMC) and a titanium disulfide, TiS2. The performance of the cells was evaluated through galvanostatic cycling and Alternating Current AC electrochemical impedance measurements. Reversible capacities were observed for both cathodes for at least tens of cycles. However, the high-voltage oxide cathode cell shows lower reversible capacity and larger fading upon cycling than the sulfide one. The AC impedance measurements revealed an increasing interfacial resistance at the cathode side for the oxide cathode inducing the capacity fading. This resistance was attributed to the intrinsic poor conductivity of NMC and interfacial reactions between the oxide material and the argyrodite electrolyte. On the contrary, the low interfacial resistance of the TiS2 cell during cycling evidences a better chemical compatibility between this active material and substituted argyrodites, allowing full cycling of the cathode material, 240 mAhg−1, for at least 35 cycles with a coulombic efficiency above 97%.
Corrugated Sheeting as a Member of a Shear Panel Under Repeated Load—Experimental Test
Authors: Korcz-Konkol Natalia , Iwicki Piotr
Published: September 2020

In stressed-skin design, the cladding stiffening effect on structures is taken into account. However, the “traditional” design is more usual, wherein this effect is neglected. Even if the diaphragm actions are not regarded, in particular cases such as big sheds (and others), the parasitic (unwanted) stressed-skin action may occur with the result of leakage or even failure. The structures of this kind have already been built. Thus, an important question arises: How can one assess them if there is a need to correct or redesign them? What kind of non-destructive approach can be used to achieve that? Experimental tests of small-scale shear panels made of trapezoidal sheeting were designed in order to observe the behaviour of the diaphragm under increasing and repeated load. The tests were oriented toward force–displacement relations and strains in selected areas of the sheeting. The results revealed nonlinear, hysteretic force–displacement behaviour of the panel and the occurrence of the persistent deflections and stresses which remain even after the unloading. The relation among the stresses, force–displacement paths and modes of failure can be potentially used in monitoring systems of existing buildings in terms of parasitic stressed-skin action.
A Modified Ridge Splitting Technique Using Autogenous Bone Blocks—A Case Series
Authors: Pénzes Dorottya , Simon Fanni , Mijiritsky Eitan , Németh Orsolya , Kivovics Márton
Published: September 2020

Background: Alveolar atrophy following tooth loss is a common limitation of rehabilitation with dental implant born prostheses. Ridge splitting is a well-documented surgical method to restore the width of the alveolar ridge prior to implant placement. The aim of this case series is to present a novel approach to ridge expansion using only autogenous bone blocks. Methods: Patients with Kennedy Class I. and II. mandibles with insufficient bone width were included in this study. Ridge splitting was carried out with the use of a piezoelectric surgery device by preparing osteotomies and after mobilization of the buccal cortical by placing an autologous bone block harvested from the retromolar region as a spacer between the buccal and lingual cortical plates. Block-grafts were stabilized by osteosynthesis screws. Implant placement was carried out after a 3-month healing period. A total of 13 implants were placed in seven augmented sites of six patients. Results: Upon re-entry, all sites healed uneventfully. Mean ridge width gain was 2.86 mm, range: 2.0–5.0 mm. Conclusions: Clinical results of our study show that the modified ridge splitting technique is a safe and predictable method to restore width of the alveolar ridge prior to implant placement.
Conditions of the Presence of Bimodal Amplitude Distribution of Two-Process Surfaces
Authors: Pawlus Pawel , Reizer Rafal , Wieczorowski Michal
Published: September 2020

Two-process surfaces are functionally important. They contain plateau and valley parts. They are created by superimpositions of two one-process textures of Gaussian probability height distributions. It is expected that the resulting two-process surface would have bimodal height probability distribution. However, typically two-process textures have unimodal ordinate distribution. The present authors developed limiting conditions of presence of bimodal ordinate distribution. These conditions depend on the material ratio at the plateau-to-valley transition (the Smq parameter), and on the ratio of heights of the plateau and valley surface parts (Spq/Svq). Generated stratified textures and measured two-process surfaces of cylinder liners were taken into consideration.
On Complete Monotonicity of Solution to the Fractional Relaxation Equation with the nth Level Fractional Derivative
Authors: Luchko Yuri
Published: September 2020

In this paper, we first deduce the explicit formulas for the projector of the nth level fractional derivative and for its Laplace transform. Afterwards, the fractional relaxation equation with the nth level fractional derivative is discussed. It turns out that, under some conditions, the solutions to the initial-value problems for this equation are completely monotone functions that can be represented in form of the linear combinations of the Mittag–Leffler functions with some power law weights. Special attention is given to the case of the relaxation equation with the second level derivative.
Group Decision-Making Based on Artificial Intelligence: A Bibliometric Analysis
Authors: Heradio Ruben , Fernandez-Amoros David , Cerrada Cristina , Cobo Manuel J.
Published: September 2020

Decisions concerning crucial and complicated problems are seldom made by a single person. Instead, they require the cooperation of a group of experts in which each participant has their own individual opinions, motivations, background, and interests regarding the existing alternatives. In the last 30 years, much research has been undertaken to provide automated assistance to reach a consensual solution supported by most of the group members. Artificial intelligence techniques are commonly applied to tackle critical group decision-making difficulties. For instance, experts’ preferences are often vague and imprecise; hence, their opinions are combined using fuzzy linguistic approaches. This paper reports a bibliometric analysis of the ample literature published in this regard. In particular, our analysis: (i) shows the impact and upswing publication trend on this topic; (ii) identifies the most productive authors, institutions, and countries; (iii) discusses authors’ and journals’ productivity patterns; and (iv) recognizes the most relevant research topics and how the interest on them has evolved over the years.
Modelling of Alumina Splat Solidification on Preheated Steel Substrate Using the Network Simulation Method
Authors: González Morales Noelia , Sánchez-Pérez Juan Francisco , Moreno Nicolás Jose Andres , Killinger Andreas
Published: September 2020

A mathematical model, consisting of a set of differential equations, for the simulation of the alumina splat solidification on steel substrate is presented. The network simulation method is used to solve the problem, which provides the temperatures and the cooling rate in the splat and substrate with a high temporal and spatial resolution for different values of the preheated substrate temperature. The results of this calculation provide important information for the design of ceramic coatings. The model design is explained in depth and simulated in open source software. As expected, the temperature evolutions in several points of the splat, an important variable to know the type of phases and the effect of the manufacturing parameters on this process, coincide with the experimental results. The model is also checked by another experimental test with tin and a bigger splat, which enables the temperature to be measured during solidification. It is worth highlighting the study of the cooling rate, a fundamental parameter to determine the phase, whether amorphous, gamma or alpha. Furthermore, a sensitive study of the mesh was included in order to optimize the computational time.