bluuebunny/crossref_metadata_2025 huggingface dataset:
Three-Phase State Estimation of a Low-Voltage Distribution Network with Kalman Filter
Authors: Napolitano Fabio , Rios Penaloza Juan Diego , Tossani Fabio , Borghetti Alberto , Nucci Carlo Alberto
Published: November 2021

The state estimation of distribution networks has long been considered a challenging task for the reduced availability of real-time measures with respect to the transmission network case. This issue is expected to be improved by the deployment of modern smart meters that can be polled at relatively short time intervals. On the other hand, the management of the information coming from many heterogeneous meters still poses major issues. If low-voltage distribution systems are of interest, a three-phase formulation should be employed for the state estimation due to the typical load imbalance. Moreover, smart meter data may not be perfectly synchronized. This paper presents the implementation of a three-phase state estimation algorithm of a real portion of a low-voltage distribution network with distributed generation equipped with smart meters. The paper compares the typical state estimation algorithm that implements the weighted least squares method with an algorithm based on an iterated Kalman filter. The influence of nonsynchronicity of measurements and of delays in communication and processing is analyzed for both approaches.
Improvement of BIPV Efficiency by Application of Highly Reflective Surfaces at the Building Envelope
Authors: Knera Dominika , Dellicompagni Pablo Roberto , Heim Dariusz
Published: November 2021

The use of concentrated solar irradiation for the improvement of electric generation improvement has been implemented on different scales, mainly in photovoltaic systems. High-concentration Fresnel lenses are widely chosen for this approach in large installations, while low-concentration systems are rather applied in medium-low scales. For the latter, the improvement on electric performance was revealed, even when no solar tracking was implemented. The presented work aims to analyse a low-concentration photovoltaic installation by a numerical approach. First, the reflective surfaces were designed geometrically considering the optimal slope determined for each month. Subsequently, different simulation techniques were used separately for prediction of solar irradiation and energy production. Three criteria were selected to analyze power generation: the highest increase in total annual solar irradiance on panels with reflective surfaces, the highest total annual solar irradiance collected, and the optimal slope of panels for the entire year. The increase in energy was found to not exceed 10% in the winter months. Whereas in the spring and summer months the energy improvement is about 15–20%. Moreover, it was observed that the temperature of the proposed concentration photovoltaic system increased significantly, reaching more than 90 °C, while for traditional PV panels it did not exceed 75 °C.
Technical and Economic Analysis of Low-Emissions Modernization of Existing Heating Plants in Poland
Authors: Hnydiuk-Stefan Anna , Otawa Aleksandra , Stefan Krzysztof , Zmarzły Dariusz
Published: November 2021

An analysis is performed with regards to technologically outdated heating plants operating in many areas where fossil fuels such as coal and gas are utilized, in order to consider the alternatives of their modernization. By application of a chart using a variety of alternatives, the economic feasibility of executing two types of modernization of heating plants are explored: a single-fuel gas–steam CHP plant and a coal-fired heating plant to a coal-fired CHP plant with a condensing turbine. This study demonstrates how the selection of modernization technology is affected, in terms of profitability, by the value and variability in time of the price relationships between energy carriers, rapidly growing charges related to CO2 emission allowances, and costs depending on other pollutant emissions that originate from the operation of electricity and heat sources powered by fossil fuels. In both technical cases of modernization, lower prices of energy carriers coupled with CO2 emissions allowances lead to higher prices of electricity that can be sold as additional products following this modernization, and consequently, the specific cost of heat production in the repowered heat sources is lowered. The calculations were performed by the application of models of heating plant modernization applying continuous time notations, which offer the determination of the most suitable time of initiation of this modernization. Such relationships would be difficult to describe in the case of the use of traditional discrete models. In the case of a simultaneous increase in the prices of all main factors affecting the cost of heat generation, such as the price of gas, electricity and CO2 emissions, the fastest modernization of the heating plant to single-fuel gas–steam CHP provides the possibility of the best economic performance.
Modifying Puree Meals in Residential Aged Care Facilities: A Multi-Centre Feasibility and Acceptability Study
Authors: Pu Dai , Choi Yuen-Yu , Chan Karen Man-Kei , Poon May Man-Wai
Published: November 2021

Purees are often recommended for older adults in residential aged care facilities (RACFs) to target swallowing difficulties and nutrition. However, they lack appeal and may have negative impacts on nutritional intake. This study investigated the subjective experiences and objective swallowing function and safety of older adults in response to a modified puree. Twenty-eight residents from three RACFs whose regular diets consisted of purees were recruited. Purees were modified to improve visual appeal by adding a commercial enzyme gellant. Each participant was observed during three puree and three modified puree meals, and completed a brief questionnaire after each meal. A videofluoroscopic swallowing study (VFSS) was performed with 16 of the participants. Compared to purees, modified purees were observed to be easier for oral processing and intake amount was higher, but participants required assistance more often and mealtimes were longer. Participants did not show preference for either type of puree. VFSS showed similar swallowing responses between the two puree types; however, a distinction was observed for modified pureed meat compared to other ingredients. Modifying puree meals in RACFs is a feasible approach to improve nutritional intake while maintaining swallowing safety, but their appeal to consumers is not definitive.
Development and Initial Validation of the PILCAST Questionnaire: Understanding Parents’ Intentions to Let Their Child Cycle or Walk to School
Authors: Forsberg Hanna , Lindqvist Anna-Karin , Forward Sonja , Nyberg Lars , Rutberg Stina
Published: November 2021

Children generally do not meet the recommendation of 60 min of daily physical activity (PA); therefore, active school transportation (AST) is an opportunity to increase PA. To promote AST, the involvement of parents seems essential. Using the theory of planned behavior (TPB), the aim was to develop and validate the PILCAST questionnaire to understand parents’ intentions to let their child cycle or walk to school. Cross-sectional sampling was performed, where 1024 responses were collected from parents. Confirmatory factor analysis indicated acceptable fit indices for the factorial structure according to the TPB, comprising 32 items grouped in 11 latent constructs. All constructs showed satisfying reliability. The regression analysis showed that the TPB explained 55.3% of parents’ intentions to let the child cycle to school and 20.6% regarding walking, increasing by a further 18.3% and 16.6%, respectively, when past behavior was added. The most influential factors regarding cycling were facilitating perceived behavioral control, positive attitudes, subjective and descriptive norms, and for walking, subjective and descriptive norms. The PILCAST questionnaire contributes to a better understanding of the psychological antecedents involving parents’ decisions to let their child cycle or walk to school, and may therefore provide guidance when designing, implementing and evaluating interventions aiming to promote AST.
Multi-Objective Optimal Allocation of River Basin Water Resources under Full Probability Scenarios Considering Wet–Dry Encounters: A Case Study of Yellow River Basin
Authors: Guan Xike , Dong Zengchuan , Luo Yun , Zhong Dunyu
Published: November 2021

Wet–dry encounters between basins and regions have an important impact on the allocation of water resources. This study proposes a multi-objective allocation model for basin water resources under full probability scenarios considering wet–dry encounters (FPS-MOWAM) to solve the problem of basin water resource allocation. In the FPS-MOWAM model, the sub-regions were merged by precipitation correlation analysis. Next, the joint probability distribution of basin runoff and region precipitation was constructed using copula functions. The possible wet–dry encounter scenarios and their probabilities were then acquired. Finally, the multi-objective allocation model of water resources was constructed using the full probability scenario for wet–dry encounters in each region. The FPS-MOWAM is calculated by the NSGA-II algorithm and the optimal water resource allocation scheme was selected using the fuzzy comprehensive evaluation method. Using the Yellow River Basin as an example, the following conclusions were obtained: (1) the Yellow River Basin can be divided into four sub-regions based on precipitation correlations: Qh-Sc (Qinghai, Sichuan), Sg-Nx-Nmg (Gansu, Ningxia, Inner Mongolia), Sxq-Sxj (Shaanxi, Shanxi), and Hn-Sd (Henan, Shandong), (2) the inconsistencies in synchronous–asynchronous encounter probabilities in the Yellow River Basin were significant (the asynchronous probabilities were 0.763), whereas the asynchronous probabilities among the four regions were 0.632, 0.932, and 0.763 under the high, medium, and low flow conditions in the Yellow River Basin respectively, and (3) the allocation of water resources tends to increase with time, allocating the most during dry years. In 2035, the expected economic benefits are between 11,982.7 billion CNY and 12,499.6 billion CNY, while the expected water shortage rate is between 2.02% and 3.43%. In 2050, the expected economic benefits are between 21,291.4 billion CNY and 21,781.3 billion CNY, while the expected water shortage rate is between 1.28% and 6.05%.
Increased Prevalence of Atopic Dermatitis in Children Aged 0–3 Years Highly Exposed to Parabens
Authors: Arafune Johichi , Tsujiguchi Hiromasa , Hara Akinori , Shimizu Yukari , Hori Daisuke , Nguyen Thao Thi Thu , Suzuki Fumihiko , Hamagishi Toshio , Yamada Yohei , Nakamura Haruki , Yoshikawa Takahiro , Hayashi Koichiro , Shibata Aki , Fukutomi Yuma , Ohya Yukihiro , Yamamoto-Hanada Kiwako , Muto Go , Hirota Ryoji , Konoshita Tadashi , Kambayashi Yasuhiro , Nakamura Hiroyuki
Published: November 2021

The prevalence of allergic diseases, such as bronchial asthma, atopic dermatitis, nasal allergies (pollinosis), and food allergies, has been increasing in many countries. The hygiene hypothesis was recently considered from the perspective of exposure to antimicrobial agents and preservatives, such as parabens (CAS number, 94-13-3). It currently remains unclear whether parabens, which are included in many daily consumer products such as cosmetics, shampoos, and personal care products as preservative antimicrobial agents, induce or aggravate allergies. Therefore, the aim of the present study was to examine the relationship between exposure to parabens and the prevalence of allergic diseases in Japanese children. The cross-sectional epidemiology of 236 children aged 0–3 years who underwent health examinations in Shika town in Japan assessed individual exposure to parabens using urinary concentrations of parabens. The results obtained showed that the prevalence of atopic dermatitis was significantly higher in children with high urinary concentrations of parabens than in those with low concentrations (p < 0.001). This relationship remained significant after adjustments for confounding factors, such as age, sex, Kaup’s index, and passive smoking (p < 0.001). In conclusion, the present results from a population study suggested a relationship between atopic dermatitis and exposure to parabens. A longitudinal study using a larger sample number and a detailed examination of atopic dermatitis, including EASI scores and exposure to parabens, will be necessary.
A Structural Equation Modelling Approach to Determine Factors of Bone Mineral Density in Korean Women
Authors: Je Minkyung , Kim Hyerim , Kim Yoona
Published: November 2021

Background: no studies have assessed the associations of nutrient intake, physical activity, age, and body mass index (BMI) with bone mineral density (BMD) using structural equation modelling (SEM) in Korean women. The aim of this study was to examine the effects of nutrient intakes, physical activity, and body mass index (BMI) on BMD in Korean premenopausal and postmenopausal women, with the SEM approach, based on the fourth and fifth Korea National Health and Nutrition Examination Surveys (KNHANES) 2008–2011. Methods: SEM analysis was performed with 4160 women (2863 premenopausal women and 1297 postmenopausal women) aged 30–75 years in order to investigate total, direct, or mediating effects of nutrient intake, physical activity, age, and BMI on BMD. Model sensitivity to external misspecification and statistical significance of SEM was determined by phantom variables and bootstrapping. Reliability assessment of the SEM was done by Cronbach’s alpha. Results: a direct effect of minerals (potassium, calcium, and phosphorus) on BMD (total femur, femoral neck, lumbar spine, and whole body) was observed in premenopausal and postmenopausal women (p = 0.045 and p = 0.048, respectively). Age and BMI showed a total effect on BMD in premenopausal and postmenopausal women (p = 0.002, respectively). Conclusions: our study suggests that mineral intake (potassium, calcium, and phosphorus), age, and BMI are major contributors to BMD in Korean premenopausal and postmenopausal women aged 30–75 years.
Effect of Water-Pipe Smoking on the Normal Development of Zebrafish
Authors: Zakaria Zain Zaki , Aladwi Shaima Ahmad , Benslimane Fatiha , Al-Absi Enas S. , Al-Shafai Mashael , Yalcin Huseyin C. , Khalil Ashraf , Moustafa Ala-Eddin Al , Al-Asmakh Maha
Published: November 2021

Background: Among all types of tobacco consumption, Water-Pipe Smoking (WPS) is the most widely used in the Middle East and second-most in several other countries. The effect of WPS on normal development is not yet fully understood, thus the aim of this study is to explore the acute toxicity effects of WPS extract on zebrafish larvae. Methods: In this study, we compared the effects of WPS smoke condensates at concentrations varying from 50 to 200 µg/mL on developmental, cardiac, and behavioural (neurotoxicity) functions. Gene expression patterns of cardiac biomarkers were also evaluated by RT-qPCR. Results: Exposing zebrafish embryos to 50, 100, 150 and 200 µg/mL WPS for three days did not affect the normal morphology of Zebrafish embryos, as the tail flicking, behavioural and locomotion assays did not show any change. However, WPS deregulated cardiac markers including atrial natriuretic peptide (ANP/NPPA) and brain natriuretic peptide (BNP/NPPB). Furthermore, it induced apoptosis in a dose-dependent manner. Conclusion: Our data demonstrate that WPS can significantly affect specific cardiac parameters during the normal development of zebrafish. Further investigations are necessary to elucidate the pathogenic outcome of WPS on different aspects of human life, including pregnancy.
Effect of Vibration Massage and Passive Rest on Recovery of Muscle Strength after Short-Term Exercise
Authors: Chwała Wiesław , Pogwizd Paweł , Rydzik Łukasz , Ambroży Tadeusz
Published: November 2021

Background: The aim of the study was to compare the effect of vibration massage and passive rest on accelerating the process of muscle recovery after short-term intense exercise. Methods: Eighty-four healthy men aged 20 to 25 years participated in the study. Study participants performed isometric (ISO-M Group) and auxotonic (AUX-M group) contraction exercise in the lower limbs. Vibration massage was administered after exercise in the first recovery period. In the same period, controls rested passively, without the support of vibration massage. To assess the effectiveness of the applied vibration, a 4-fold measurement of the maximum force of the muscles involved in the exercise was performed under conditions of isometric contractions on a leg press machine set at an angle of 45° degrees upwards. Results: Differences in maximum strength during isometric contraction were found compared to baseline in favor of the groups subjected to the experimental vibration massage. Differences were demonstrated in muscle strength between the study groups (p < 0.005). The second period of passive rest in all groups did not bring significant changes in the values of maximal lower limb strength. Conclusions: Properly selected characteristics of the vibration effect can be an effective method in accelerating recovery and regaining lost motor capabilities of muscle groups fatigued by exercise. This offers the potential to shorten rest periods between sets of repetitions in training or between training units.
Anxiety, Low Self-Esteem and a Low Happiness Index Are Associated with Poor School Performance in Chilean Adolescents: A Cross-Sectional Analysis
Authors: Zapata-Lamana Rafael , Sanhueza-Campos Cristian , Stuardo-Álvarez Marcia , Ibarra-Mora Jessica , Mardones-Contreras Marcela , Reyes-Molina Daniel , Vásquez-Gómez Jaime , Lasserre-Laso Nicole , Poblete-Valderrama Felipe , Petermann-Rocha Fanny , Parra-Rizo Maria Antonia , Cigarroa Igor
Published: November 2021

Objective: To analyze the relationship between anxiety, self-esteem, happiness index and primary school students’ academic performance in Chilean adolescents from the Biobío province. Methodology: 733 (46.1% girls; 12 (1.3 years)) public primary school students that completed the 2018 Health and School Performance Survey carried out in the Biobío province were included in this cross-sectional analysis. The BECK Anxiety Inventory (BAI) was used to measure anxiety while happiness index and self-esteem were measured using the subjective happiness scale and the Rosenberg self-esteem scale, respectively. School performance was measured by grade point average (GPA) of language, math, physical education and cumulative GPA, and behavior associated with cognition in the school context was also considered. The relationship between mental health indicators and school performance was investigated using a one-way ANOVA and Pearson correlation. Results: In comparison to students with low anxiety levels and high self-esteem and happiness levels, students with higher anxiety levels, lower self-esteem and happiness levels perceived themselves as having memory problems. They were also slower to solve math problems, had a shorter attention span in class and presented more difficulties in solving complex tasks, as well as being more nervous during testing. These students also got the lowest grade point average in math, language and physical education. Conclusions: High anxiety levels, low self-esteem and low happiness levels were associated with lower school performance and weaker behavior associated with cognition in Chilean adolescents. Implementing plans of emotional education and mental health could improve academic achievement.
The Impact of Gastrointestinal Symptoms on Patients’ Well-Being: Best–Worst Scaling (BWS) to Prioritize Symptoms of the Gastrointestinal Symptom Score (GIS)
Authors: Mühlbacher Axel Christian , Kaczynski Anika
Published: November 2021

Background: The gastrointestinal symptom score (GIS) is used in a standardized form to ascertain dyspeptic symptoms in patients with functional dyspepsia in clinical practice. As a criterion for evaluating the effectiveness of a treatment, the change in the summed total point value is used. The total score ranges from 0 to 40 points, in which a higher score represents a more serious manifestation of the disease. Each symptom is included with equal importance in the overall evaluation. The objective of this study was to test this assumption from a patients’ perspective. Our aim was to measure the priorities of patients for the ten gastrointestinal symptoms by using best–worst scaling. Method: A best–worst scaling (BWS) object scaling (Case 1) was applied. Therefore, the symptoms of the GIS were included in a questionnaire using a fractional factorial design (BIBD—balanced incomplete block design). In each choice set, the patients selected the component that had the most and the least impact on their well-being. The BIB design generated a total of 15 choice sets, which each included four attributes. Results: In this study, 1096 affected patients were asked for their priorities regarding a treatment of functional dyspepsia and motility disorder. Based on the data analysis, the symptoms abdominal cramps (SQRT (B/W): −1.27), vomiting (SQRT (B/W): −1.07) and epigastric pain (SQRT (B/W): −0.76) were most important and thus have the greatest influence on the well-being of patients with functional dyspepsia and motility disorders. In the middle range are the symptoms nausea (SQRT (B/W): −0.69), acid reflux/indigestion (SQRT (B/W): −0.29), sickness (SQRT (B/W): −0.26) and retrosternal discomfort (SQRT (B/W): 0.26), whereas the symptoms causing the least impact are the feeling of fullness (SQRT (B/W): 0.80), early satiety (SQRT (B/W): 1.54) and loss of appetite (SQRT(B/W): 1.95). Discussion: Unlike the underlying assumption of the GIS, the BWS indicated that patients did not weight the 10 symptoms equally. The results of the survey show that the three symptoms of vomiting, abdominal cramps and epigastric pain are weighted considerably higher than symptoms such as early satiety, loss of appetite and the feeling of fullness. The evaluation of the BWS data has illustrated, however, that the restrictive assumption of GIS does not reflect the reality of dyspeptic patients. Conclusions: In conclusion, a preference-based GIS is necessary to make valid information about the real burden of illness and to improve the burden of symptoms in the indication of gastrointestinal conditions. The findings of the BWS demonstrate that the common GIS is not applicable to represent the real burden of disease. The results suggest the potential modification of the established GIS by future research using a stated preference study.
Public Stigma of COVID-19 and Its Correlates in the General Population of China
Authors: Zhang Tian-Ming , Fang Qi , Yao Hao , Ran Mao-Sheng
Published: November 2021

This study aimed to examine the profile of COVID-19-related public stigma and its correlates in the general population of China. A cross-sectional online survey was conducted in China from 7 May to 25 May in 2020. A total of 1212 participants from the general population completed the survey measuring their stigmatizing attitudes towards COVID-19, as well as knowledge and causal attributions of COVID-19. Univariate and multivariate analyses were performed to examine the correlates of COVID-19-related public stigma. A total of 31.8% of participants endorsed stigmatization towards people with COVID-19. Those who were of older age (t = −3.97, p < 0.001), married (F = 3.04, p < 0.05), had a lower level of education (F = 8.11, p < 0.001), and a serious psychological response (F = 3.76, p < 0.05) reported significantly higher scores of public stigma. Dangerousness (B = 0.047, p < 0.001), fear (B = 0.059, p < 0.001), anger (B = 0.038, p < 0.01), and responsibility (B = 0.041, p < 0.001) were positively associated with public stigma. This study shows that public stigma related to COVID-19 is prevalent in the general population of China. Actions against public stigma need to contain the spread of misinformation about COVID-19, alter inappropriate attributions, alleviate unfavorable reactions, and provide psychosocial support for the public.
Hemodynamic Effects of Permanent His Bundle Pacing Compared to Right Ventricular Pacing Assessed by Two-Dimensional Speckle-Tracking Echocardiography
Authors: Michalik Jedrzej , Dabrowska-Kugacka Alicja , Kosmalska Katarzyna , Moroz Roman , Kot Adrian , Lewicka Ewa , Szolkiewicz Marek
Published: November 2021

We compared the effects of right ventricular (RVP; n = 26) and His bundle (HBP; n = 24) pacing in patients with atrioventricular conduction disorders and preserved LVEF. Postoperatively (1D), and after six months (6M), the patients underwent global longitudinal strain (GLS) and peak systolic dispersion (PSD) evaluation with 2D speckle-tracking echocardiography, assessment of left atrial volume index (LAVI) and QRS duration (QRSd), and sensing/pacing parameter testing. The RVP threshold was lower than the HBP threshold at 1D (0.65 ± 0.13 vs. 1.05 ± 0.20 V, p < 0.001), and then it remained stable, while the HBP threshold increased at 6M (1.05 ± 0.20 vs. 1.31 ± 0.30 V, p < 0.001). The RVP R-wave was higher than the HBP R-wave at 1D (11.52 ± 2.99 vs. 4.82 ± 1.41 mV, p < 0.001). The RVP R-wave also remained stable, while the HBP R-wave decreased at 6M (4.82 ± 1.41 vs. 4.50 ± 1.09 mV, p < 0.02). RVP QRSd was longer than HBP QRSd at 6M (145.0 ± 11.1 vs. 112.3 ± 9.3 ms, p < 0.001). The absolute value of RVP GLS decreased at 6M (16.32 ± 2.57 vs. 14.03 ± 3.78%, p < 0.001), and HBP GLS remained stable. Simultaneously, RVP PSD increased (72.53 ± 24.15 vs. 88.33 ± 30.51 ms, p < 0.001) and HBP PSD decreased (96.28 ± 33.99 vs. 84.95 ± 28.98 ms, p < 0.001) after 6 months. RVP LAVI increased (26.73 ± 5.7 vs. 28.40 ± 6.4 mL/m2, p < 0.05), while HBP LAVI decreased at 6M (30.03 ± 7.8 vs. 28.73 ± 8.7 mL/m2, p < 0.01). These results confirm that HBP does not disrupt ventricular synchrony and provides advantages over RVP.
GPI-80 Augments NF-κB Activation in Tumor Cells
Authors: Takeda Yuji , Kurota Yuta , Kato Tomoyuki , Ito Hiromi , Araki Akemi , Nara Hidetoshi , Saitoh Shinichi , Tanaka Nobuyuki , Tsuchiya Norihiko , Asao Hironobu
Published: November 2021

Recent studies have discovered a relationship between glycosylphosphatidylinositol (GPI)-anchored protein 80 (GPI-80)/VNN2 (80 kDa GPI-anchored protein) and malignant tumors. GPI-80 is known to regulate neutrophil adhesion; however, the action of GPI-80 on tumors is still obscure. In this study, although the expression of GPI-80 mRNA was detectable in several tumor cell lines, the levels of GPI-80 protein were significantly lower than that in neutrophils. To clarify the function of GPI-80 in tumor cells, GPI-80-expressing cells and GPI-80/VNN2 gene-deleted cells were established using PC3 prostate cancer cells. In GPI-80-expressing cells, GPI-80 was mainly detected in vesicles. Furthermore, soluble GPI-80 in the conditioned medium was associated with the exosome marker CD63 and was also detected in the plasma obtained from prostate cancer patients. Unexpectedly, cell adhesion and migration of GPI-80-expressing PC3 cells were not modulated by anti-GPI-80 antibody treatment. However, similar to the GPI-80 family molecule, VNN1, the pantetheinase activity and oxidative state were augmented in GPI-80-expressing cells. GPI-80-expressing cells facilitated non-adhesive proliferation, slow cell proliferation, NF-κB activation and IL-1β production. These phenomena are known to be induced by physiological elevation of the oxidative state. Thus, these observations indicated that GPI-80 affects various tumor responses related to oxidation.
Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish
Authors: Chen Shu-Wei , Lv Wu-Hong , Wu Kun , Chen Guang-Hui , Chen Fang , Song Chang-Chun , Luo Zhi
Published: November 2021

Nano-sized zinc oxide (nano-ZnO) affects lipid deposition, but its absorption patterns and mechanisms affecting lipid metabolism are still unclear. This study was undertaken to investigate the molecular mechanism of nano-ZnO absorption and its effects on lipid metabolism in the intestinal tissues of a widely distributed freshwater teleost yellow catfish Pelteobagrus fulvidraco. We found that 100 mg/kg dietary nano-ZnO (H-Zn group) significantly increased intestinal Zn contents. The zip6 and zip10 mRNA expression levels were higher in the H-Zn group than those in the control (0 mg/kg nano-ZnO), and zip4 mRNA abundances were higher in the control than those in the L-Zn (50 mg/kg nano-ZnO) and H-Zn groups. Eps15, dynamin1, dynamin2, caveolin1, and caveolin2 mRNA expression levels tended to reduce with dietary nano-ZnO addition. Dietary nano-ZnO increased triglyceride (TG) content and the activities of the lipogenic enzymes glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and isocitrate dehydrogenase (ICDH), upregulated the mRNA abundances of lipogenic genes 6pgd, fatty acid synthase (fas), and sterol regulatory element binding protein 1 (srebp1), and reduced the mRNA expression of farnesoid X receptor (fxr) and small heterodimer partner (shp). The SHP protein level in the H-Zn group was lower than that in the control and the L-Zn group markedly. Our in vitro study indicated that the intestinal epithelial cells (IECs) absorbed nano-ZnO via endocytosis, and nano-Zn-induced TG deposition and lipogenesis were partially attributable to the endocytosis of nano-ZnO in IECs. Mechanistically, nano-ZnO-induced TG deposition was closely related to the metal responsive transcription factor 1 (MTF-1)-SHP pathway. Thus, for the first time, we found that the lipogenesis effects of nano-ZnO probably depended on the key gene shp, which is potentially regulated by MTF1 and/or FXR. This novel signaling pathway of MTF-1 through SHP may be relevant to explain the toxic effects and lipotoxicity ascribed to dietary nano-ZnO addition.
A Quadruplex qPCR for Detection and Differentiation of Classic and Natural Recombinant Myxoma Virus Strains of Leporids
Authors: Abade dos Santos Fábio A. , Carvalho Carina L. , Parra Francisco , Dalton Kevin P. , Peleteiro Maria C. , Duarte Margarida D.
Published: November 2021

A natural recombinant myxoma virus (referred to as ha-MYXV or MYXV-Tol08/18) emerged in the Iberian hare (Lepus granatensis) and the European rabbit (Oryctolagus cuniculus) in late 2018 and mid-2020, respectively. This new virus is genetically distinct from classic myxoma virus (MYXV) strains that caused myxomatosis in rabbits until then, by acquiring an additional 2.8 Kbp insert within the m009L gene that disrupted it into ORFs m009L-a and m009L-b. To distinguish ha-MYXV from classic MYXV strains, we developed a robust qPCR multiplex technique that combines the amplification of the m000.5L/R duplicated gene, conserved in all myxoma virus strains including ha-MYXV, with the amplification of two other genes targeted by the real-time PCR systems designed during this study, specific either for classic MYXV or ha-MYXV strains. The first system targets the boundaries between ORFs m009L-a and m009L-b, only contiguous in classic strains, while the second amplifies a fragment within gene m060L, only present in recombinant MYXV strains. All amplification reactions were validated and normalized by a fourth PCR system directed to a housekeeping gene (18S rRNA) conserved in eukaryotic organisms, including hares and rabbits. The multiplex PCR (mPCR) technique described here was optimized for Taqman® and Evagreen® systems allowing the detection of as few as nine copies of viral DNA in the sample with an efficiency > 93%. This real-time multiplex is the first fast method available for the differential diagnosis between classic and recombinant MYXV strains, also allowing the detection of co-infections. The system proves to be an essential and effective tool for monitoring the geographical spread of ha-MYXV in the hare and wild rabbit populations, supporting the management of both species in the field.
Insights into Intra-Tumoral Heterogeneity: Transcriptional Profiling of Chemoresistant MPM Cell Subpopulations Reveals Involvement of NFkB and DNA Repair Pathways and Contributes a Prognostic Signature
Authors: Cioce Mario , Sacconi Andrea , Pass Harvey I. , Canino Claudia , Strano Sabrina , Blandino Giovanni , Fazio Vito Michele
Published: November 2021

Chemoresistance is a hallmark of malignant pleural mesothelioma (MPM) management and the expression of ALDH1A3 is responsible for the survival and activity of MPM chemoresistant cell subpopulations (ALDHbright cells). We enriched mesothelioma ALDHbright cells to near homogeneity by FACS sorting and an Aldefluor assay and performed unbiased Affymetrix gene expression profiling. Viability and ELISA assays were used to rule out significant apoptosis in the sorted cell subpopulations and to assess target engagement by butein. Statistical analysis of the results, pathway enrichment and promoter enrichment were employed for the generation of the data. Q-RTPCR was used to validate a subset of the identified, modulated mRNAs In this work, we started from the observation that the mRNA levels of the ALDH1A3 isoform could prognostically stratify MPM patients. Thus, we purified MPM ALDHbright cells from NCI-H2595 cells and interrogated their gene expression (GES) profile. We analyzed the GES of the purified cells at both a steady state and upon treatment with butein (a multifunctional tetrahydroxy-chalcone), which abates the ALDHbright cell number, thereby exerting chemo-sensitizing effects in vitro and in vivo. We identified 924 genes modulated in a statistically significant manner as a function of ALDH status and of the response to the inhibitor. Pathway and promoter enrichment identified the molecular determinant of high ALDH status and how butein treatment altered the molecular portrait of those chemoresistant cell subpopulations. Further, we unraveled an eighteen-gene signature with high prognostic significance for MPM patients, and showed that most of the identified prognostic contributors escaped the analysis of unfractionated samples. This work proves that digging into the unexplored field of intra-tumor heterogeneity (ITH) by working at the cell subpopulation level may provide findings of prognostic relevance, in addition to mechanistic insights into tumor resistance to therapy.
Controlling Equilibrium Morphologies of Bimetallic Nanostructures Using Thermal Dewetting via Phase-Field Modeling
Authors: Kwak Taejin , Kim Dongchoul
Published: November 2021

Herein, we report a computational model for the morphological evolution of bimetallic nanostructures in a thermal dewetting process, with a phase-field framework and superior optical, physical, and chemical properties compared to those of conventional nanostructures. The quantitative analysis of the simulation results revealed nano-cap, nano-ring, and nano-island equilibrium morphologies of the deposited material in thermal dewetting, and the morphologies depended on the gap between the spherical patterns on the substrate, size of the substrate, and deposition thickness. We studied the variations in the equilibrium morphologies of the nanostructures with the changes in the shape of the substrate pattern and the thickness of the deposited material. The method described herein can be used to control the properties of bimetallic nanostructures by altering their equilibrium morphologies using thermal dewetting.
Influence of Alloy Atoms on Substitution Properties of Hydrogen by Helium in ZrCoH3
Authors: Wang Panpan , Cao Qilong , You Yuwei , Kong Xiangshan , Wu Xuebang , Liu Changsong
Published: November 2021

Intermetallic alloy ZrCo is a good material for storing tritium (T). However, ZrCo is prone to disproportionation reactions during the process of charging and discharging T. Alloying atoms are often added in ZrCo, occupying the Zr or Co site, in order to restrain disproportionation reactions. Meanwhile, T often decays into helium (He), and the purity of T seriously decreases once He escapes from ZrCo. Therefore, it is necessary to understand the influence of alloying atoms on the basic stability property of He. In this work, we perform systematical ab initio calculations to study the stability property of He in ZrCoH3 (ZrCo adsorbs the H isotope, forming ZrCoH3). The results suggest that the He atom will undergo displacements of 0.31 and 0.12 Å when it substitutes for Co and Zr, respectively. In contrast, the displacements are very large, at 0.67–1.09 Å, for He replacing H. Then, we introduce more than 20 alloying atoms in ZrCo to replace Co and Zr in order to examine the influence of alloying atoms on the stability of He at H sites. It is found that Ti, V, Cr, Mn, Fe, Zn, Nb, Mo, Tc, Ru, Ta, W, Re, and Os replacing Co can increase the substitution energy of H by the He closest to the alloying atom, whereas only Cr, Mn, Fe, Mo, Tc, Ru, Ta, W, Re, and Os replacing Co can increase the substitution energy of H by the He next closest to the alloying atom. The influence of the alloying atom substituting Zr site on the substitution energies is inconspicuous, and only Nb, Mo, Ru, Ta, and W increase the substitution energies of H by the He closest to the alloying atom. The increase in the substitution energy may suggest that these alloy atoms are conducive to fix the He atom in ZrCo and avoid the reduction in tritium purity.
Hygric Properties of Machine-Made, Historic Clay Bricks from North-Eastern Poland (Former East Prussia): Characterization and Specification for Replacement Materials
Authors: Tunkiewicz Maria , Misiewicz Joanna , Sikora Pawel , Chung Sang-Yeop
Published: November 2021

This paper deals with the hygric characterization of early 20th century machine-made clay bricks, representative of great number of historical buildings in north-eastern Poland. Heritage buildings have a high potential for adaptive reuse, which is strictly connected with an urge for knowledge about the properties of these existing building envelopes. To better understand the hygric behavior of historic buildings, various experimental laboratory tests, including density, water absorption, compressive strength and freeze-thaw resistance, were conducted. In order to assess the microstructural characteristics of the tested bricks, mercury intrusion porosimetry (MIP) and X-ray micro-computed tomography (micro-CT) tests were performed. These tests were conducted on clay bricks from historic buildings, as well as on those that are currently being produced, in order to identify the relationship between the materials used in the past and the replacements produced presently. This paper addresses the lack of systematic application of existing standards for evaluating the state of the conservation of historic bricks and for establishing the specifications for replacement bricks. The results of conducted study and further research will be the basis for creating a historic materials database. It would be a useful tool for selecting bricks that correspond with the historically used materials and help to maintain homogenous structure of the restored buildings.
Carbon Nanotubes: Current Perspectives on Diverse Applications in Targeted Drug Delivery and Therapies
Authors: Rahamathulla Mohamed , Bhosale Rohit R. , Osmani Riyaz A. M. , Mahima Kasturi C. , Johnson Asha P. , Hani Umme , Ghazwani Mohammed , Begum Mohammed Y. , Alshehri Sultan , Ghoneim Mohammed M. , Shakeel Faiyaz , Gangadharappa Hosahalli V.
Published: November 2021

Current discoveries as well as research findings on various types of carbon nanostructures have inspired research into their utilization in a number of fields. These carbon nanostructures offer uses in pharmacy, medicine and different therapies. One such unique carbon nanostructure includes carbon nanotubes (CNTs), which are one-dimensional allotropes of carbon nanostructure that can have a length-to-diameter ratio greater than 1,000,000. After their discovery, CNTs have drawn extensive research attention due to their excellent material properties. Their physical, chemical and electronic properties are excellent and their composites provide great possibilities for enormous nanometer applications. The current study provides a systematic review based on prior literature review and data gathered from various sources. The various research studies from many research labs and organizations were systematically retrieved, collected, compiled and written. The entire collection and compilation of this review concluded the use of CNT approaches and their efficacy and safety for the treatment of various diseases such as brain tumors or cancer via nanotechnology-based drug delivery, phototherapy, gene therapy, antiviral therapy, antifungal therapy, antibacterial therapy and other biomedical applications. The current review covers diverse applications of CNTs in designing a range of targeted drug delivery systems and application for various therapies. It concludes with a discussion on how CNTs based medicines can expand in the future.
Structural Retrofitting of Corroded Reinforced Concrete Beams Using Bamboo Fiber Laminate
Authors: Awoyera Paul Oluwaseun , Nworgu Tobechukwu Austin , Shanmugam Balaji , Prakash Arunachalam Krishna , Mansouri Iman , Romero Lenin Miguel Bendezu , Hu Jong-Wan
Published: November 2021

Corrosion creates a significant degradation mechanism in reinforced concrete (RC) structures, which would require a high cost of maintenance and repair in affected buildings. However, as the cost of repairing corrosion-damaged structures is high, it is therefore pertinent to develop alternative eco-friendly and sustainable methods. In this study, structural retrofitting of corroded reinforced concrete beams was performed using bamboo fiber laminate. Three reinforced normal weight concrete beams were produced, two of which were exposed to laboratory simulated corrosion medium, and the remaining one sample served as control. Upon completion of the corrosion cycle, one of the two corroded beams was retrofitted externally with a prefabricated bamboo fiber laminate by bonding the laminate to the beam surface with the aid of an epoxy resin. The three beams were subjected to loading on a four-point ultimate testing machine, and the loads with corresponding deflections were recorded through the entire load cycle of the beams. Finally, the mass loss of embedded steel reinforcements was determined to measure the effect of corrosion on the beams and the steel. The result showed that corroded beams strengthened with bamboo laminates increase the bearing capacity. Using a single bamboo laminate in the tensile region of the corroded beam increased the ultimate load capacity of the beam up to 21.1% than the corroded beam without retrofit. It was demonstrated in this study that the use of bamboo fiber polymer for strengthening destressed RC beams is a more sustainable approach than the conventional synthetic fibers.
Thermochemical Laser-Induced Periodic Surface Structures Formation by Femtosecond Laser on Hf Thin Films in Air and Vacuum
Authors: Belousov Dmitrij A. , Bronnikov Kirill A. , Okotrub Konstantin A. , Mikerin Sergey L. , Korolkov Victor P. , Terentyev Vadim S. , Dostovalov Alexander V.
Published: November 2021

Thermochemical laser-induced periodic surface structures (TLIPSS) are a relatively new type of periodic structures formed in the focal area of linear polarized laser radiation by the thermally stimulated reaction of oxidation. The high regularity of the structures and the possibility of forming high-ordered structures over a large area open up possibilities for the practical application for changing the optical and physical properties of materials surface. Since the mechanism of formation of these structures is based on a chemical oxidation reaction, an intriguing question involves the influence of air pressure on the quality of structure formation. This paper presents the results on the TLIPSS formation on a thin hafnium film with fs IR laser radiation at various ambient air pressures from 4 Torr to 760 Torr. Despite the decrease in the oxygen content in the ambient environment by two orders of magnitude, the formation of high-ordered TLIPSS (dispersion in the LIPSS orientation angle δθ < 5°) with a period of ≈700 nm occurs within a wide range of parameters variation (laser power, scanning speed). This behavior of TLIPSS formation is in agreement with experimental data obtained earlier on the study of the kinetics of high-temperature oxidation of hafnium at various oxygen pressures.
Deep Learning for Object Detection, Classification and Tracking in Industry Applications
Authors: Wang Dadong , Wang Jian-Gang , Xu Ke
Published: November 2021

Object detection, classification and tracking are three important computer vision techniques [...]
Erratum: Jamšek et al. Gaussian Mixture Models for Control of Quasi-Passive Spinal Exoskeletons. Sensors 2020, 20, 2705
Authors: Jamšek Marko , Petrič Tadej , Babič Jan
Published: November 2021

There was an error in the original article [...]
In-Orbit Attitude Determination of the UVSQ-SAT CubeSat Using TRIAD and MEKF Methods
Authors: Finance Adrien , Dufour Christophe , Boutéraon Thomas , Sarkissian Alain , Mangin Antoine , Keckhut Philippe , Meftah Mustapha
Published: November 2021

Ultraviolet and infrared sensors at high quantum efficiency on-board a small satellite (UVSQ-SAT) is a CubeSat dedicated to the observation of the Earth and the Sun. This satellite has been in orbit since January 2021. It measures the Earth’s outgoing shortwave and longwave radiations. The satellite does not have an active pointing system. To improve the accuracy of the Earth’s radiative measurements and to resolve spatio-temporal fluctuations as much as possible, it is necessary to have a good knowledge of the attitude of the UVSQ-SAT CubeSat. The attitude determination of small satellites remains a challenge, and UVSQ-SAT represents a real and unique example to date for testing and validating different methods to improve the in-orbit attitude determination of a CubeSat. This paper presents the flight results of the UVSQ-SAT’s attitude determination. The Tri-Axial Attitude Determination (TRIAD) method was used, which represents one of the simplest solutions to the spacecraft attitude determination problem. Another method based on the Multiplicative Extended Kalman Filter (MEKF) was used to improve the results obtained with the TRIAD method. In sunlight, the CubeSat attitude is determined at an accuracy better than 3° (at one σ) for both methods. During eclipses, the accuracy of the TRIAD method is 14°, while it reaches 10° (at one σ) for the recursive MEKF method. Many future satellites could benefit from these studies in order to validate methods and configurations before launch.
vTrust: An IoT-Enabled Trust-Based Secure Wireless Energy Sharing Mechanism for Vehicular Ad Hoc Networks
Authors: Awan Kamran Ahmad , Din Ikram Ud , Almogren Ahmad , Kim Byung-Seo , Altameem Ayman
Published: November 2021

Vehicular Ad hoc Network (VANET) is a modern concept that enables network nodes to communicate and disseminate information. VANET is a heterogeneous network, due to which the VANET environment exposes to have various security and privacy challenges. In the future, the automobile industry will progress towards assembling electric vehicles containing energy storage batteries employing these resources to travel as an alternative to gasoline/petroleum. These vehicles may have the capability to share their energy resources upon the request of vehicles having limited energy resources. In this article, we have proposed a trust management-based secure energy sharing mechanism, named vTrust, which computes the trust degree of nodes to authenticate nodes. The proposed mechanism is a multi-leveled centralized approach utilizing both the infrastructure and vehicles to sustain a secure environment. The proposed vTrust can aggregate and propagate the degree of trust to enhance scalability. The node that requests to obtain the energy resources may have to maintain a specified level of trust threshold for earning resources. We have also evaluated the performance of the proposed mechanism against several existing approaches and determine that the proposed mechanism can efficiently manage a secure environment during resource sharing by maintaining average malicious nodes detection of 91.3% and average successful energy sharing rate of 89.5%, which is significantly higher in comparison to the existing approaches.