bluuebunny/crossref_metadata_2025 huggingface dataset:
Why female birds of prey are larger than males
Authors: Schoenjahn Jonny , Pavey Chris R , Walter Gimme H
Published: December 2019

The causes of the reversed sexual size dimorphism (RSD; females larger than males) in birds of prey are subject to a centuries-old, passionate debate. A crucial difficulty is to distinguish whether the postulated benefits derive from the proposed causal process(es) or are incidental. After reviewing the existing literature, we present a methodology that overcomes this difficulty and renders unnecessary any speculative a priori distinctions between evolved function and incidental effects. We can thus justify the following novel version of the well-known nest defence hypothesis as the most likely to explain the phenomenon in all birds of prey that show RSD: if the female predominates in actively defending the eggs and young against predators, then she is the heavier sex, and her relatively greater body mass is adaptive. That is, heavier females are favoured (independently of males) by natural selection. The attractiveness of this hypothesis is that it has the potential to explain the phenomenon in all raptors exhibiting RSD, can deal with the exceptional cases in this group, explains the direction of the dimorphism, focuses on a key factor in the reproductive success of most raptors, is parsimonious, i.e. does not require supporting hypotheses, and is supported by a substantial body of evidence.
Long-Term Ecological Research and Evolving Frameworks of Disturbance Ecology
Authors: Gaiser Evelyn E , Bell David M , Castorani Max C N , Childers Daniel L , Groffman Peter M , Jackson C Rhett , Kominoski John S , Peters Debra P C , Pickett Steward T A , Ripplinger Julie , Zinnert Julie C
Published: December 2019

Detecting and understanding disturbance is a challenge in ecology that has grown more critical with global environmental change and the emergence of research on social–ecological systems. We identify three areas of research need: developing a flexible framework that incorporates feedback loops between social and ecological systems, anticipating whether a disturbance will change vulnerability to other environmental drivers, and incorporating changes in system sensitivity to disturbance in the face of global changes in environmental drivers. In the present article, we review how discoveries from the US Long Term Ecological Research (LTER) Network have influenced theoretical paradigms in disturbance ecology, and we refine a framework for describing social–ecological disturbance that addresses these three challenges. By operationalizing this framework for seven LTER sites spanning distinct biomes, we show how disturbance can maintain or alter ecosystem state, drive spatial patterns at landscape scales, influence social–ecological interactions, and cause divergent outcomes depending on other environmental changes.
Lack of Increased Risk of Lymphoma by Thiopurines or Biologics in Japanese Patients with Inflammatory Bowel Disease: A Large-Scale Administrative Database Analysis
Authors: Kobayashi Taku , Uda Akihito , Udagawa Eri , Hibi Toshifumi
Published: December 2019

Background and Aims

Patients with inflammatory bowel diseases may have higher incidences of non-melanoma skin cancers and non-Hodgkin lymphoma, potentially linked to underlying disease and treatments. This analysis assessed incidence rates of these malignancies in Japanese patients with ulcerative colitis or Crohn’s disease, and their association with thiopurine and/or anti-tumor necrosis factor-α treatment, using data from a nationwide administrative database in Japan.

Methods

Patients diagnosed with inflammatory bowel disease without malignancy were identified from the Medical Data Vision database. Incident cases of non-melanoma skin cancers and non-Hodgkin lymphoma diagnosed after prescription of thiopurine and/or anti-tumor necrosis factor-α were identified between April 2008 and January 2018. Age- and sex-adjusted incidence rate ratios were calculated relative to the total treated patient population.

Results

A total of 75 673 eligible patients were identified at the index date. Thiopurine prescription with or without anti-tumor necrosis factor-α agents increased incidence rate ratios for non-melanoma skin cancers relative to the overall population (3.39 and 4.03, respectively). There were no notable differences in non-Hodgkin lymphoma incidence relative to the total population in any treatment subgroup, regardless of prescription of thiopurine and/or anti-tumor necrosis factor-α (all incidence rate ratios, ~1).

Conclusions

There is no evidence for an increased incidence of non-Hodgkin lymphoma attributable to thiopurine or anti-tumor necrosis factor-α treatment in Japanese patients with inflammatory bowel disease. The impact of racial differences on non-Hodgkin lymphoma incidences should be considered. Thiopurine therapy may be a risk factor for non-melanoma skin cancers in Japanese patients.
Midstory Removal Facilitates Growth but Reduces Competitiveness of Oak Reproduction Prior to and after Shelterwood Establishment Cutting
Authors: Hackworth Zachary J , Lhotka John M , Stringer Jeffrey W
Published: December 2019

This study examined the influence of midstory removal applied 9 years prior to a shelterwood establishment cut on survival, growth, and competitive status of black oak (Quercus velutina Lam.), white oak (Quercus alba L.), and an oak competitor, red maple (Acer rubrum L.), immediately before and at 6 years following shelterwood cutting on intermediate productivity sites in the Central Hardwood Forest Region. We also examined height and density distributions of the regenerating cohort to quantify seedling establishment and to elucidate relative oak competitiveness at each time period. Although oak seedlings were taller 9 years after midstory removal than in undisturbed controls, the midstory removal treatment reduced the frequency of free-to-grow oaks (relative to understory competitors) and increased the size of red maple and relative density of large competitors. Height growth of all three species following establishment cutting was increased by prior midstory removal and dependent on seedling competitive position immediately before shelterwood establishment. Additionally, we observed increased seedling densities 6 years following shelterwood establishment cutting and greater white oak seedling establishment when midstory removal preceded the shelterwood treatment.
Comparative in vitro Study of the biological activity and chemical composition extracts of Helicteres isora L. obtained by water and subcritical water extraction
Authors: Didar Zohreh
Published: December 2019

Objectives

Subcritical water extraction technique is considered as an environmentally extraction technique. The aim of this study was to compare the different characteristics of water extract and subcritical water extract of Helicteres isora L.

Materials and Methods

Water extraction was performed under the following conditions: 25°C, 24 h, and solid-to-water ratio 1:30. Subcritical water extract was carried out under specific conditions (pressure = 10 bar, temperature = 160°C, solid-to-water ratio = 1: 30, time = 30 min). Chemical composition analysis was performed using GC–Mass chromatography. Anti-biofilm activity in the terms of anti-attach and removal of biofilm were assessed using the ELISA reader method and reading absorbance at 570 nm. Anti-microbial activity against Bacillus cereus, Staphylococcus aureus, Staphylococcus saprophyticus, and Bacillus subtilis was investigated by measurement of inhibitory zone diameter. Anti-enzymatic and antioxidant properties were also assessed.

Results

The results of GC–Mass analysis showed some components extracted in subcritical method which were absent in water extract such as octadecanoic acid, hexadecanoic acid, and berberin. Antioxidant activity of the two tested extracts revealed that subcritical water extract had more antioxidant capacity than water extract (P ≤ 0.05). The two tested extracts exhibited anti-enzymatic activity against polyphenol oxidase enzyme with better performance of subcritical water extract. Anti-biofilm activity of the two extracts implies that, in the case of preventing biofilm formation, both extracts had similar efficiency but in the removal of biofilm, subcritical water extract showed better performance. Both extracts had anti-microbial activity against B. cereus, S. aureus, S. saprophyticus, and B. subtilis with better performance of subcritical water extract. Anti-enzymatic assay also showed similar results.

Conclusions

Subcritical water extract of H. isora showed more antioxidant activity as well as anti-biofilm, anti-bacterial, and anti-enzymatic activity rather than ordinary water extract.
A Closer Look at Loneliness: Why Do First-Generation Migrants Feel More Lonely Than Their Native Dutch Counterparts?
Authors: ten Kate Rowan L F , Bilecen Başak , Steverink Nardi
Published: December 2019

Background and Objectives

Along with the current aging demographics in the Netherlands, the number of older first-generation migrants is also increasing. Despite studies suggesting a higher quantity of social contacts of migrants, loneliness is more common among migrants as compared to native Dutch. We theorize that migrants experience more emotional and social loneliness due to a lower satisfaction with social relationships and lower participation in social activities, respectively, compared to their native counterparts.

Research Design and Methods

We use data from Statistics Netherlands (N = 7,920) with first-generation migrants aged 40 years and older and their Dutch counterparts. Contact frequency, household composition, satisfaction with social relationships, relationship quality with the partner, and social activities, are used as main predictors and separate regression models for social and emotional loneliness are analyzed.

Results

Compared to the native Dutch, first-generation migrants are both socially and emotionally more lonely. Migrants have a similar contact frequency as the native Dutch, but are less satisfied with their social relationships, which contributes to their higher emotional, social, and overall loneliness. Migrants engage less in social activities but this does not put them at additional risk of loneliness.

Discussion and Implications

Migrants experience more social and emotional loneliness and are less satisfied with their social relationships compared to their native counterparts. Interventions should focus on reducing both social and emotional loneliness among older migrants. Specific attention should be paid to fostering satisfying social interactions. Additionally, encouraging migrants to broaden their social network may reduce social loneliness.
Influence of ecosystem changes on harvestable resources at high latitudes
Authors: Haug Tore , Dolgov Andrey , Filin Anatoly , Fossheim Maria , Huse Geir , Shamray Evgeny , Stiansen Jan Erik , Trofimov Alexander
Published: December 2019

Results from investigations of the Barents Sea environment and biological resources were presented at a Russian–Norwegian Symposium in Murmansk, Russia on 5–7 June 2018. The thematic focus of the symposium was “Influence of ecosystem changes on harvestable resources at high latitudes”. Contributions to the symposium were organized under five theme sessions: oceanography, plankton, benthos, fishes, and top predators. From the rich spectrum of symposium contributions, five articles appear in this supplemental issue.
Neurofunctional Differences Related to Methamphetamine and Sexual Cues in Men With Shorter and Longer Term Abstinence Methamphetamine Dependence
Authors: Chen Shubao , Huang Shucai , Yang Cheng , Cai Weifu , Chen Hongxian , Hao Wei , Liu Tieqiao , Wang Xuyi , Worhunsky Patrick D , Potenza Marc N
Published: December 2019

Background

Stimulant use and sexual behaviors have been linked in behavioral and epidemiological studies. Although methamphetamine-related neurofunctional differences have been investigated, few studies have examined neural responses to drug and sexual cues with respect to shorter or longer term methamphetamine abstinence in individuals with methamphetamine dependence.

Methods

Forty-nine men with shorter term methamphetamine abstinence, 50 men with longer term methamphetamine abstinence, and 47 non–drug-using healthy comparison men completed a functional magnetic resonance imaging cue-reactivity task consisting of methamphetamine, sexual, and neutral visual cues.

Results

Region-of-interest analyses revealed greater methamphetamine cue–related activation in shorter term methamphetamine abstinence and longer term methamphetamine abstinence individuals relative to healthy comparison men in the ventromedial prefrontal cortex. A significant interaction of group and condition in the anterior insula was found. Relative to healthy comparison participants, both shorter term methamphetamine abstinence and longer term methamphetamine abstinence groups displayed greater sexual cue–related anterior insula activation relative to methamphetamine cues and neutral cues, but there were no differences between shorter term methamphetamine abstinence and longer term methamphetamine abstinence groups in anterior insula responses. Subsequent whole-brain analyses indicated a group-by-condition interaction with longer term methamphetamine abstinence participants showing greater sexual-related activation in the left superior frontal cortex relative to healthy comparison men. Shorter term methamphetamine abstinence participants showed greater superior frontal cortex activation to sexual relative to neutral cues, and longer term methamphetamine abstinence participants showed greater superior frontal cortex activation to sexual relative to neutral and methamphetamine cues.

Conclusions

The findings suggest that abstinence from methamphetamine may alter how individuals respond to drug and sexual cues and thus may influence drug use and sexual behaviors. Given the use of methamphetamine for sexual purposes and responses to natural vs drug rewards for addiction recovery, the findings may have particular clinical relevance.
Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation
Authors: Espinosa Juan Carlos , Marín-Moreno Alba , Aguilar-Calvo Patricia , Benestad Sylvie L , Andreoletti Olivier , Torres Juan María
Published: December 2019

Although experimental transmission of bovine spongiform encephalopathy (BSE) to pigs and transgenic mice expressing pig cellular prion protein (PrPC) (porcine PrP [PoPrP]–Tg001) has been described, no natural cases of prion diseases in pig were reported. This study analyzed pig-PrPC susceptibility to different prion strains using PoPrP-Tg001 mice either as animal bioassay or as substrate for protein misfolding cyclic amplification (PMCA). A panel of isolates representatives of different prion strains was selected, including classic and atypical/Nor98 scrapie, atypical-BSE, rodent scrapie, human Creutzfeldt-Jakob-disease and classic BSE from different species. Bioassay proved that PoPrP-Tg001-mice were susceptible only to the classic BSE agent, and PMCA results indicate that only classic BSE can convert pig-PrPC into scrapie-type PrP (PrPSc), independently of the species origin. Therefore, conformational flexibility constraints associated with pig-PrP would limit the number of permissible PrPSc conformations compatible with pig-PrPC, thus suggesting that pig-PrPC may constitute a paradigm of low conformational flexibility that could confer high resistance to the diversity of prion strains.
Structural Uncertainty in Onchocerciasis Transmission Models Influences the Estimation of Elimination Thresholds and Selection of Age Groups for Seromonitoring
Authors: Hamley Jonathan I D , Walker Martin , Coffeng Luc E , Milton Philip , de Vlas Sake J , Stolk Wilma A , Basáñez Maria-Gloria
Published: December 2019

Background

The World Health Organization recommends monitoring Onchocerca volvulus Ov16 serology in children aged <10 years for stopping mass ivermectin administration. Transmission models can help to identify the most informative age groups for serological monitoring and investigate the discriminatory power of serology-based elimination thresholds. Model predictions depend on assumed age-exposure patterns and transmission efficiency at low infection levels.

Methods

The individual-based transmission model, EPIONCHO-IBM, was used to assess (1) the most informative age groups for serological monitoring using receiver operating characteristic curves for different elimination thresholds under various age-dependent exposure assumptions, including those of ONCHOSIM (another widely used model), and (2) the influence of within-human density-dependent parasite establishment (included in EPIONCHO-IBM but not ONCHOSIM) on positive predictive values for different serological thresholds.

Results

When assuming EPIONCHO-IBM exposure patterns, children aged <10 years are the most informative for seromonitoring; when assuming ONCHOSIM exposure patterns, 5–14 year olds are the most informative (as published elsewhere). Omitting density-dependent parasite establishment results in more lenient seroprevalence thresholds, even for higher baseline infection prevalence and shorter treatment durations.

Conclusions

Selecting appropriate seromonitoring age groups depends critically on age-dependent exposure patterns. The role of density dependence on elimination thresholds largely explains differing EPIONCHO-IBM and ONCHOSIM elimination predictions.
Ruminal motility, reticuloruminal fill, and eating patterns in steers exposed to ergovaline
Authors: Ahn Gyuchul , Ricconi Kara , Avila Suelen , Klotz James L , Harmon David L
Published: December 2019

Fescue toxicosis is problematic for growing steers, causing lower DMI and productivity when fed endophyte-infected (E+) tall fescue. A complete understanding of underlying mechanisms of how fescue toxicosis affects growing steers is lacking. Therefore, the overall objective of this multiexperiment study was to determine whether ruminally dosed ergovaline (ERV) affects rumen motility, rumen contents, and eating patterns. In Exp. 1, an 8-h period to assess ruminal motility began 4 h after feeding by monitoring pressure changes using a wireless system for 21 d. Eight ruminally cannulated steers (283 kg BW) were pair fed with alfalfa cubes (1.5 × NEm) and assigned to endophyte free (E−; 0 μg ERV/kg BW/d) or E+ treatment (20 μg ERV/kg BW/d). Overall, E+ steers had more frequent rumen contractions (Seed P = 0.05 and day of feeding P = 0.02). On days 7 to 9, both treatments showed lower frequencies and E− steers had greater amplitude of contractions (P < 0.001) that corresponded with decreased DMI. In Exp. 2, steers remained in pairs assigned in Exp. 1 (322 kg BW), but reversed seed treatments while increasing ERV levels (titrated 0, 5, 10, 15, and 20 μg ERV/kg BW/d over 57 d). There were no differences between E− and E+ for frequency (P = 0.137) or amplitude of contractions (P = 0.951), but increasing ERV dosage, decreased frequency (P = 0.018) and amplitude (P = 0.005), coinciding with lower DMI. In Exp. 3, 8 steers (589 kg) were pair fed and ruminally dosed 15 μg ERV/kg BW/d, and rumen motility data were collected for 21 d. E− steers showed higher amplitude and lower frequency of contractions than E+ steers with seed (P < 0.001), day (P < 0.001), and seed × day (P < 0.04) effects, but rumen fill was not different between E− and E+ (P > 0.29). Serum prolactin concentrations were lower in E+ steers in Exp. 1 to 3. Eating patterns of pair-fed E− and E+ steers were relatively slower in E+ than E− (Exp. 4) by measuring every 2 h across 24 h. Number of meals were higher in E+ than E− steers, but meal duration and meal size were not different between treatments. Rumen content (DM%) tended to be higher in E+ than in E− when steers were fed once a day (P = 0.07), but there was no difference for rumen content (DM%) when E− and E+ steers were fed 12 times a day (P = 0.13). These results suggest the changes in rumen fill associated with fescue toxicosis may be driven more by changes in feeding behavior and eating pattern rather than by changes in motility.
Changes in Morphometric Traits of Ground Beetles Along Urbanization Gradients
Authors: Papp Dalma , Mizser Szabolcs , Nagy Leila , Vidic Andreas , Simon Edina , Tóthmérész Béla
Published: December 2019

Urbanization has a significant impact on abiotic and biotic factors in nature. We examined the morphometric characters of four carabid species (Abax parallelepipedus, Carabus scheidleri, Carabus violaceus, and Pterostichus oblongopunctatus) along urbanization gradients in and around the cities of Vienna (Austria) and Debrecen (Hungary). We found significant differences among urban, suburban, and rural areas in the parameters of antennomers, the maxillary palpus, the labial palpus, and the length of the tibia and the elytra of the carabids studied. We also found significant differences between males and females based on the parameters of antennomers, the maxillary palpus, the labial palpus, the femur, and the elytra. An interaction between urbanization and sex was found in the case of antennomers, the maxillary palpus, the labial palpus, the femur, and the elytra. Our findings suggested that in the cases of species from Carabini tribus the parameters of antennomers, the maxillary palpus, and the elytra could be useful for assessing the effects of urbanization because these morphometric characters responded sensitively to the environmental stress, whereas the most useful parameters are those of antennomers and the tibia for the species of Pterostichini tribus. Our findings also revealed that females are more sensitive to environmental stress than males.
A rare case of neglected clasped thumb of 5-year-old girl treated with simple z-plasty, two tendons transplantation, and followed up for 17 years
Authors: Smail Mohamed Hadi Al , Aloulou Mohammad , Zrek Abdulmouain
Published: December 2019

Congenital malformations of the thumb greatly affect the performance of the hand and the upper limb. The conditions associated with atrophic thumb represent a wide spectrum of abnormalities, ranging from a complete absence to a mere small size of the thumb. A 5-year-old Caucasian female presented with congenital clasped thumb deformity with hypoplastic thumb and with an absence of extrinsic extensors extensor pollicis longus and brevis and with stable carpometacarpal joint. We managed this case by transferring the extensor indicis proprius tendon and flexor digitorum superficialis of digitus medicinalis (the ring finger)—which is not common—to compensate for the absence of extension and abduction tendons of the thumb. After 17 years, the result was satisfactory for the patient and her family. She was able to use her hand for writing and doing normal daily work without feeling any disability.
Four bottlenecks restrict colonization and invasion by the pathogen Ralstonia solanacearum in resistant tomato
Authors: Planas-Marquès Marc , Kressin Jonathan P , Kashyap Anurag , Panthee Dilip R , Louws Frank J , Coll Nuria S , Valls Marc
Published: December 2019

Ralstonia solanacearum is a bacterial vascular pathogen causing devastating bacterial wilt. In the field, resistance against this pathogen is quantitative and is available for breeders only in tomato and eggplant. To understand the basis of resistance to R. solanacearum in tomato, we investigated the spatio-temporal dynamics of bacterial colonization using non-invasive live monitoring techniques coupled to grafting of susceptible and resistant varieties. We found four ‘bottlenecks’ that limit the bacterium in resistant tomato: root colonization, vertical movement from roots to shoots, circular vascular bundle invasion, and radial apoplastic spread in the cortex. Radial invasion of cortical extracellular spaces occurred mostly at late disease stages but was observed throughout plant infection. This study shows that resistance is expressed in both root and shoot tissues, and highlights the importance of structural constraints to bacterial spread as a resistance mechanism. It also shows that R. solanacearum is not only a vascular pathogen but spreads out of the xylem, occupying the plant apoplast niche. Our work will help elucidate the complex genetic determinants of resistance, setting the foundations to decipher the molecular mechanisms that limit pathogen colonization, which may provide new precision tools to fight bacterial wilt in the field.
Luminous Red Novae: population models and future prospects
Authors: Howitt George , Stevenson Simon , Vigna-Gómez Alejandro , Justham Stephen , Ivanova Natasha , Woods Tyrone E , Neijssel Coenraad J , Mandel Ilya
Published: December 2019

A class of optical transients known as Luminous Red Novae (LRNe) have recently been associated with mass ejections from binary stars undergoing common-envelope evolution. We use the population synthesis code COMPAS to explore the impact of a range of assumptions about the physics of common-envelope evolution on the properties of LRNe. In particular, we investigate the influence of various models for the energetics of LRNe on the expected event rate and light curve characteristics, and compare with the existing sample. We find that the Galactic rate of LRNe is ∼0.2 yr−1, in agreement with the observed rate. In our models, the luminosity function of Galactic LRNe covers multiple decades in luminosity and is dominated by signals from stellar mergers, consistent with observational constraints from iPTF and the Galactic sample of LRNe. We discuss how observations of the brightest LRNe may provide indirect evidence for the existence of massive (>40 M⊙) red supergiants. Such LRNe could be markers along the evolutionary pathway leading to the formation of double compact objects. We make predictions for the population of LRNe observable in future transient surveys with the Large Synoptic Survey Telescope and the Zwicky Transient Facility. In all plausible circumstances, we predict a selection-limited observable population dominated by bright, long-duration events caused by common envelope ejections. We show that the Large Synoptic Survey Telescope will observe 20–750 LRNe per year, quickly constraining the luminosity function of LRNe and probing the physics of common-envelope events.
Early fate of exogenous promoters in E. coli
Authors: Yousuf Malikmohamed , Iuliani Ilaria , Veetil Reshma T , Seshasayee Aswin Sai Narain , Sclavi Bianca , Cosentino Lagomarsino Marco
Published: December 2019

Gene gain by horizontal gene transfer is a major pathway of genome innovation in bacteria. The current view posits that acquired genes initially need to be silenced and that a bacterial chromatin protein, H-NS, plays a role in this silencing. However, we lack direct observation of the early fate of a horizontally transferred gene to prove this theory. We combine sequencing, flow cytometry and sorting, followed by microscopy to monitor gene expression and its variability after large-scale random insertions of a reporter gene in a population of Escherichia coli bacteria. We find that inserted promoters have a wide range of gene-expression variability related to their location. We find that high-expression clones carry insertions that are not correlated with H-NS binding. Conversely, binding of H-NS correlates with silencing. Finally, while most promoters show a common level of extrinsic noise, some insertions show higher noise levels. Analysis of these high-noise clones supports a scenario of switching due to transcriptional interference from divergent ribosomal promoters. Altogether, our findings point to evolutionary pathways where newly-acquired genes are not necessarily silenced, but may immediately explore a wide range of expression levels to probe the optimal ones.
AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development
Authors: Motomura Kazuki , Arae Toshihiro , Araki-Uramoto Haruka , Suzuki Yuya , Takeuchi Hidenori , Suzuki Takamasa , Ichihashi Yasunori , Shibata Arisa , Shirasu Ken , Takeda Atsushi , Higashiyama Tetsuya , Chiba Yukako
Published: December 2019

Development of pollen, the male gametophyte of flowering plants, is tightly controlled by dynamic changes in gene expression. Recent research to clarify the molecular aspects of pollen development has revealed the involvement of several transcription factors in the induction of gene expression. However, limited information is available about the factors involved in the negative regulation of gene expression to eliminate unnecessary transcripts during pollen development. In this study, we revealed that AtNOT1 is an essential protein for proper pollen development and germination capacity. AtNOT1 is a scaffold protein of the AtCCR4–NOT complex, which includes multiple components related to mRNA turnover control in Arabidopsis. Phenotypic analysis using atnot1 heterozygote mutant pollen showed that the mature mutant pollen failed to germinate and also revealed abnormal localization of nuclei and a specific protein at the tricellular pollen stage. Furthermore, transcriptome analysis of atnot1 heterozygote mutant pollen showed that the downregulation of a large number of transcripts, along with the upregulation of specific transcripts required for pollen tube germination by AtNOT1 during late microgametogenesis, is important for proper pollen development and germination. Overall, our findings provide new insights into the negative regulation of gene expression during pollen development, by showing the severely defective phonotype of atnot1 heterozygote mutant pollen.
VOLUME CT DOSE INDEX AND DOSE-LENGTH PRODUCT VALUES ACCORDING TO FACILITY SIZE IN JAPAN
Authors: Matsunaga Yuta , Kondo Yuya , Kobayashi Kenichi , Kobayashi Masanao , Minami Kazuyuki , Suzuki Shoichi , Chida Koichi , Asada Yasuki
Published: December 2019

The aim of this study was to investigate differences in volume computed tomography dose index (CTDIvol) and dose-length product (DLP) values according to facility size in Japan. A questionnaire survey was sent to 3000 facilities throughout Japan. Data from each facility were collected including bed number, computed tomography (CT) scan parameters employed and the CTDIvol and/or DLP values displayed on the CT scanner during each examination. The CTDIvol and DLP for 11 adult and 6 paediatric CT examinations were surveyed. Comparison of CTDIvol and DLP values of each examination according to facility size revealed key differences in CT dose between small and large facilities. This study highlights the importance of lowering the dose of coronary artery examination with contrast agent in smaller facilities and of lowering the dose of adult and paediatric head CT without contrast agent in larger facilities. The results of this study are valid in Japan.
Improving Substituted Performance in India: Attending to the Loopholes
Authors: Thakur Kanishk , Thakur Dushyant
Published: December 2019

With the passing of the Specific Relief (Amendment) Act, 2018, the primary remedy for breach of contract was changed to specific performance from that of damages. The amendment brought in many other path-breaking changes, along with the introduction of a provision on substituted performance under Indian contract law. Now, a non-defaulting party will have a statutory remedy to either perform the contract himself or get it performed by a third party, the cost of which must be borne by the defaulting party. Despite being a welcomed provision, the faulty legislative drafting of the provision has left many issues unresolved. The authors, in this article, have tried to point out those issues. Thereafter, using the cross-jurisdictional analysis and taking into consideration the jurisprudence developed on past contractual practices, the authors have come up with suggestions to improve the provision.
Response: Recent Development regarding Judicial Review of Legislative Process in Israel
Authors: Ilan Gonen
Published: December 2019

In an article published recently in the Statue Law Review,1 Prof. Shucheng Wang has examined the deferential approach to judicial review of the legislative process as adopted by the CFA,2 due to the Leung3 case. In this short response, I focus on two issues that are not mentioned in Prof. Wang’s article but that are crucial for understanding recent developments concerning judicial review of the legislative process in Israel by Israel’s High Court of Justice (HCJ). First, this response will present the most recent, and more crucial, ruling of the HCJ from late 2017 in the Quantinsky v. The Israeli Knesset (2017)4 which established a new precedent and in which, for the first time in Israel’s history, a new law was invalidated due to flaws in the legislative process. Second, I wish to emphasize the specific type of law that was invalidated and that is more prone to flaws in the legislative process: The Omnibus Law of Arrangement in the State Economy, which includes hundreds of budget statutes, and is characterized by a very unique and hasty legislative process. Therefore, this response wishes to complement Prof. Wang’s thesis and provide an update regarding the Israeli HCJ judicial approach of due process of legislation.
Conifer and broadleaved trees differ in branch allometry but maintain similar functional balances
Authors: Zhang Lan , Chen Yajun , Hao Guangyou , Ma Keping , Bongers Frans , Sterck Frank J
Published: December 2019

Conifers and broadleaved trees coexist in temperate forests and are expected to differ in partitioning strategies between leaf and stem. We compare functional balances between water loss and water supply, and between sugar production and sugar transport/storage, and associate these with xylem growth to better understand how they contribute to these life form strategies. We sampled canopy branches from 14 common species in a temperate forest in northeast China and measured xylem area, phloem area, ray area, ray percentage, dry wood density, xylem conductivity and mean xylem growth rate for branch stems, and the leaf area and specific leaf area for leaves, and calculated the leaf-specific conductivity. Conifers and broadleaved trees did not differ significantly in tissue areas, xylem growth rate and the relation between phloem area and leaf area. Conifers had higher xylem area but lower ray area relative to leaf area. For the same xylem conductivity, phloem area and ray parenchyma area did not differ between conifers and broadleaved trees. Xylem growth rate was similar relative to leaf area and phloem area. Our results indicate that conifers tend to develop more xylem area per leaf area and more tracheid area at the cost of ray parenchyma area, probably to compensate for the low water transport ability of tracheid-based xylem. The divergent strategies between conifers and broadleaved tree species in leaf area and xylem area partitioning probably lead to the convergence of partitioning between leaf area and phloem area. Consequently, conifers tend to consume rather than store carbon to achieve a similar xylem expansion per year as coexisting broadleaved trees.
Quantitative Ordinal Scale Estimates of Plant Disease Severity: Comparing Treatments Using a Proportional Odds Model
Authors: Chiang K. S. , Liu H. I. , Chen Y. L. , El Jarroudi M. , Bock C. H.
Published: December 2019

Studies in plant pathology, agronomy, and plant breeding requiring disease severity assessment often use quantitative ordinal scales (i.e., a special type of ordinal scale that uses defined numeric ranges); a frequently used example of such a scale is the Horsfall-Barratt scale. Parametric proportional odds models (POMs) may be used to analyze the ratings obtained from quantitative ordinal scales directly, without converting ratings to percent area affected using range midpoints of such scales (currently a standard procedure). Our aim was to evaluate the performance of the POM for comparing treatments using ordinal estimates of disease severity relative to two alternatives, the midpoint conversions (MCs) and nearest percent estimates (NPEs). A simulation method was implemented and the parameters of the simulation estimated using actual disease severity data from the field. The criterion for comparison of the three approaches was the power of the hypothesis test (the probability to reject the null hypothesis when it is false). Most often, NPEs had superior performance. The performance of the POM was never inferior to using the MC at severity <40%. Especially at low disease severity (≤10%), the POM was superior to using the MC method. Thus, for early onset of disease or for comparing treatments with severities <40%, the POM is preferable for analyzing disease severity data based on quantitative ordinal scales when comparing treatments and at severities >40% is equivalent to other methods.
Access to Contraception in Local Health Departments, Four Midwest States, 2017–2018
Authors: Satterwhite Catherine Lindsey , French Valerie , Allison Molly , Honderick Tanya , Ramaswamy Megha
Published: December 2019

(Abstracted from Contraception 2019;99:363–367)

Barriers to contraception use are well known and include education, cost, and access. Successful efforts to expand access for women of reproductive age to a broad range of contraceptive methods focus on intrauterine devices (IUDs) and implants, which are long-acting reversible methods.
Sexual Satisfaction Changes Reported by Men After Their Partners' Robotic-assisted Laparoscopic Sacrocolpopexies
Authors: Culligan Patrick J. , Haughey Sean , Lewis Christa , Priestley Jennifer , Salamon Charbel
Published: December 2019

(Abstracted from Female Pelvic Med Reconstr Surg 2019;25:365–368)

Most women with symptomatic pelvic organ prolapse (POP) experience improved sexual function after successful POP repair. Little is known about the impact of their reconstructive surgery on their male partners' sexual function and satisfaction.
Neurologic Outcome After Prematurity: Perspectives of Parents and Clinicians
Authors: Lemmon Monica E. , Huffstetler Hanna , Barks Mary Carol , Kirby Christine , Katz Madelaine , Ubel Peter A. , Docherty Sharron L. , Brandon Debra
Published: December 2019

(Abstracted from Pediatrics 2019;144(1):e20183819)

Clinicians and parents often need to make time-sensitive and high-stakes decisions when caring for premature infants. Yet, little is known about how each group discusses, understands, and makes these decisions.
On Step 1 Mania, USMLE Score Reporting, and Financial Conflict of Interest at the National Board of Medical Examiners
Authors: Carmody J. Bryan , Rajasekaran Senthil K.
Published: December 2019

Though intended to inform a binary decision on initial medical licensure, the United States Medical Licensing Examination (USMLE) is frequently used for screening candidates for residency positions. Some have argued that reporting results as pass/fail would honor the test’s purpose while preventing inappropriate use. To date, the USMLE’s sponsor organizations have declined to make such a change. In this Perspective, the authors examine the history and mission of the National Board of Medical Examiners (NBME), trace the rise of “Step 1 mania,” and consider the current financial incentives for the NBME in implementing a pass/fail score-reporting policy.

The NBME was founded in 1915 to address the lack of interstate reciprocity in medical licensure examination. With the creation of the USMLE in 1992, a single pathway for licensure was established, and the organization’s original mission was achieved. Yet even after fulfilling its primary purpose, the NBME—classified as a nonprofit organization—has seen its revenues rise dramatically over the past 2 decades. Much of the increased revenue is derived from test products and services not required for medical licensure, with sales driven by the increasing importance of Step 1 scores in residency selection. Revenue from these products and services would likely decline if the NBME reported Step 1 results as pass/fail.

A financial conflict of interest occurs when a judgment concerning a primary interest may be influenced by a secondary interest, such as financial gain. The data presented here demonstrate that the NBME has a conflict of interest in its current score-reporting policy. Possible remedies, such as disclosure, recusal, divestiture, and restructuring, are considered.
The Civil Rights Doctor, Revisited
Authors: Mullan Fitzhugh
Published: December 2019

The author recalls the summer of 1965, which he spent in Holmes County, Mississippi, as a medical civil rights worker. The poverty, bravery, ignorance, brotherhood, racism, hate, and love he experienced that summer led him to conclude he would become a civil rights doctor. When he returned to medical school in Chicago, the author and his classmates began organizing students around the idea of social justice. They intended to take on society’s big problems even as their medical education ignored them.

More than 50 years later, the author reflects on the sense of mission that attracts many people to medicine. A mission more than the desire to heal. A mission to recognize and address the inequities in the world and, more to the point, in access to health and health care. Medical schools have a unique role or “social mission” in that they are the only institutions that can build doctors for the future. The culture of the medical school is a powerful influence on the values of its graduates and, ultimately, the physicians of the country. The articulated, cerebrated, strategized mission that a medical school selects for itself has an enormous influence on who gets to be a doctor and what the values of that doctor are in the future, and that is why, the author argues, medical schools must incorporate social mission.

To achieve this vision, medical education must move beyond Abraham Flexner’s 20th-century legacy. This is not to disown Flexner, science, or research but to rethink medical education based on the equity challenges that confront our population now. Physicians and the institutions that train them need to see social mission as a living part of the medical skill set rather than an elective perspective exercised by some who are particularly compassionate.
HUMAN AMNIOTIC MEMBRANE TO CLOSE RECURRENT, HIGH MYOPIC MACULAR HOLES IN PATHOLOGIC MYOPIA WITH AXIAL LENGTH OF ≥30 mm
Authors: Caporossi Tomaso , Pacini Bianca , De Angelis Lorenzo , Barca Francesco , Peiretti Enrico , Rizzo Stanislao
Published: December 2019

Purpose:

To assess the effectiveness of the human amniotic membrane plug for recurrent high myopic macular hole (MH) that already underwent pars plana vitrectomy with internal limiting membrane peeling and gas endotamponade.

Methods:

Sixteen eyes of 16 patients with recurrent high myopic MH were enrolled. A 23-gauge pars plana vitrectomy was performed. Human amniotic membrane plugs were implanted under the neuroretina inside the MH. Twenty percent sulfur hexafluoride or air was used as endotamponades. The patients were instructed to maintain facedown position for 5 days after surgery.

Results:

Optical coherence tomography examinations showed that the MHs closed in 15 of the 16 patients (93.75%) 2 weeks after one surgical intervention, and in 100% of patients after second intervention. Mean best-corrected visual acuity improved from 1 logarithm of the minimum angle of resolution (20/200) to 0.67 logarithm of the minimum angle of resolution (20/100) 6 months after surgery. Best-corrected visual acuity remained stable during the 12-month follow-up. One patient had human amniotic membrane plug dislocation after gas absorption that needed a second intervention with new AM plug implantation. No adverse events were reported during the 12-month follow-up.

Conclusion:

The first case series of recurrent high myopic MH was reported, assessing the effectiveness of the human amniotic membrane plug to close recurrent MHs in pathologic myopia. All the cases were successful with encouraging best-corrected visual acuity recovery.
MACULAR HOLE FORMATION ASSOCIATED WITH INTENSE PULSED LIGHT THERAPY
Authors: Maganti Nenita , Kalbag Neil S. , Gill Manjot K.
Published: December 2019

Purpose:

To describe the first case of macular hole formation following intense pulsed light therapy.

Methods:

This is a retrospective case report. A 68-year-old woman presented with blurry vision that occurred 1 day following intense pulsed light therapy for hair removal.

Results:

A full thickness macular hole, which had not been previously documented was noted on fundus exam. Ocular coherence tomography confirmed a full-thickness macular hole.

Discussion:

Laser treatment for hair removal has been reported to cause ocular injuries including iris atrophy, pupillary distortion, cataracts, and uveitis. Intense pulsed light therapy, a specific type of laser therapy, has been reported to cause iris transillumination defects, anterior uveitis, and pupil abnormalities. Our case is the first to describe a macular hole secondary to intense pulsed light therapy. The etiology of the macular hole is postulated to be secondary to possible thermal injury with absorption of energy at the level of the retinal pigment epithelium. This emphasizes the role and importance of preventative measures, such as protective glasses in reducing the risk of ocular injuries.
Identification of Specific Tumor Markers in Vulvar Carcinoma Through Extensive Human Papillomavirus DNA Characterization Using Next Generation Sequencing Method
Authors: Thomas Jacques , Leufflen Léa , Chesnais Virginie , Diry Ségolène , Demange Jessica , Depardieu Claude , Bani Mohamed Amine , Marchal Frédéric , Charra-Brunaud Claire , Merlin Jean-Louis , Leroux Agnès , Sastre-Garau Xavier , Harlé Alexandre
Published: December 2019

Objectives

A subset of vulvar carcinomas (VC) are associated with human papillomavirus (HPV) DNA. This trait can be used to identify tumor markers for patient's follow-up. A large diversity of HPV prevalence in VC has been reported, but no data are available concerning the insertional HPV status in this tumor type. Therefore, we have used an innovative next generation sequencing (NGS)-based CaptHPV method able to provide an extensive characterization of HPV DNA in tumors.

Material and Methods

Tumor tissue specimens from 55 patients with VC were analyzed using p16 immunohistochemistry, in situ hybridization, polymerase chain reaction, and CaptHPV-NGS assays.

Results

Our analyses showed that 8 (14.5%) of 55 cases were associated with HPV 16 DNA. No other HPV genotypes were identified. The HPV genome was in a free episomal state only in one case and both episomal and integrated into the tumor cell genome in 7. There was a single insertion in 5 cases and multiple sites, scattered at different chromosomal loci in two. ISH data suggest that some of these might reflect tumor heterogeneity. Viral integration targeted cellular genes among which were TP63, CCDC148, LOC100133091, PKP1, and POLA2. Viral integration at the PKP1 locus was associated with partial gene deletion, and no PKP1 protein was detected in tumor tissue.

Conclusions

Using the NGS-based innovative capture-HPV approach, we established a cartography of HPV 16 DNA in 8 VC cases and identified novel genes targeted by integration that may be used as specific tumor markers. In addition, we established a rationale strategy for optimal characterization of HPV status in VC.