bluuebunny/crossref_metadata_2025 huggingface dataset:
Technical Case Report of a Cranioplasty With ex vivo Frozen Ostoblastic Bone Graft From Large Skull Metastasis
Authors: Perng Pang-Shuo , Lee Po-Hsuan , Hsu Hao-Hsiang , Huang Chi-Chen , Huang Chih-Yuan , Lee Jung-Shun
Published: September 2021

Objective: Liquid nitrogen cryotherapy has shown efficacy in the treatment of bone tumors of the extremities with good oncologic and functional outcomes. However, its application in metastatic skull tumors has been rarely reported and whether the adjuvant radiotherapy affects the future bone healing is not yet explored. We report an immediate cranioplasty with the resected osteoblastic bone, which underwent ex vivo cryotherapy, and discuss the surgical techniques and postoperative images.

Methods: A 58-year-old man with esophageal adenocarcinoma, undergoing chemoradiotherapy, presented with a rapidly enlarging scalp mass for 5 months. Imaging revealed an enhancing mass, centered in the frontal skull bone with extracranial and intracranial invasion, suggestive of osteoblastic metastasis. After preoperative transarterial embolization, the tumor was excised en bloc. Immediate cranioplasty was performed with the osteoblastic bone graft after ex vivo cryotherapy. It was soaked in liquid nitrogen for 20 min, thawed at room temperature for 15 min, and soaked in povidone-iodine solution for 10 min. Then, the bone graft was fixed to its original place. Pathologic examination revealed metastasis originating from the esophagus. He underwent adjuvant radiotherapy for local tumor control.

Results: He had an uneventful clinical course without any neurologic deficit. Brain imaging during the six-month follow-up showed no tumor recurrence and partial bony union.

Conclusions: Cranioplasty using an autologous bone graft with ex vivo cryotherapy was helpful in the reconstruction of osteoblastic metastatic skull tumor treatment. It was a simple and cost-effective procedure that achieved satisfactory cosmetic results without negatively impacting bone healing, even after adjuvant radiotherapy.
Pathology of Bovine Tuberculosis in Three Breeds of Dairy Cattle and Spoligotyping of the Causative Mycobacteria in Ethiopia
Authors: Ambaw Mulualem , Gelalcha Benti Deresa , Bayissa Berecha , Worku Adane , Yohannis Aster , Zewude Aboma , Ameni Gobena
Published: September 2021

Different breeds of cattle were observed to have a variable degree of susceptibility to bovine tuberculosis (bTB). The screening of bTB was conducted on 720 dairy cattle consisting of three breeds using the single intradermal cervical comparative tuberculin (SICCT) test. Besides this, 43 SICCT test-positive cattle were used to compare the severity of the pathology of bTB among the three breeds and to identify the causative mycobacteria using spoligotyping. The overall SICCT test positivity was 17.92% (129/720) by pooling all animals in the three farms. There was a significant difference in SICCT test positivity among the three breeds (χ2 = 71.06; p < 0.001); the highest (25.34%) was recorded in the crossbreed followed by the Boran breed (10.08%), while the least (3.14%) was recorded in the Jersey breed. On other hand, the highest median pathology score (10.0, interquartile range, IQR = 6.0–17.0) was recorded in Boran followed by cross (5.0, IQR = 3.5–7.5), while the least (3.0, IQR = 2.25–3.0) was recorded in Jersey. Thus, the difference in the median pathology scores was significant [Kruskal Wallis χ(2)2 = 18.78, p < 0.001] among the three breeds. Furthermore, multivariate analysis using ordinal logistic regression by considering age, sex, breed, reproductive status, and location of the farms also showed a significant [χ(2)2 = 11.97, p < 0.01] difference in pathology scores among the three breeds of cattle. Even at a single-herd level at Holeta, the difference in severity of pathology between the Boran and crossbreeds was significant (U = 33.5; p < 0.01). Culture positivity was 39% in 108 suspicious tissues. Fourteen Mycobacterium bovis (M. bovis) and two Mycobacterium tuberculosis (M. tuberculosis) were isolated from the lesions. All the 14 M. bovis isolates belonged to SB0912, while the two M. tuberculosis belonged to SIT54. In conclusion, although the frequency of the SICCT test positivity was high in the crossbreed, a more severe pathology was observed on the Boran (zebu) breed. In addition M. tuberculosis was isolated from TB lesions of dairy cattle, demonstrating the role of M. tuberculosis in causing TB in cattle.
Nidoviruses in Reptiles: A Review
Authors: Parrish Kate , Kirkland Peter D. , Skerratt Lee F. , Ariel Ellen
Published: September 2021

Since their discovery in 2014, reptile nidoviruses (also known as serpentoviruses) have emerged as significant pathogens worldwide. They are known for causing severe and often fatal respiratory disease in various captive snake species, especially pythons. Related viruses have been detected in other reptiles with and without respiratory disease, including captive and wild populations of lizards, and wild populations of freshwater turtles. There are many opportunities to better understand the viral diversity, species susceptibility, and clinical presentation in different species in this relatively new field of research. In captive snake collections, reptile nidoviruses can spread quickly and be associated with high morbidity and mortality, yet the potential disease risk to wild reptile populations remains largely unknown, despite reptile species declining on a global scale. Experimental studies or investigations of disease outbreaks in wild reptile populations are scarce, leaving the available literature limited mostly to exploring findings of naturally infected animals in captivity. Further studies into the pathogenesis of different reptile nidoviruses in a variety of reptile species is required to explore the complexity of disease and routes of transmission. This review focuses on the biology of these viruses, hosts and geographic distribution, clinical signs and pathology, laboratory diagnosis and management of reptile nidovirus infections to better understand nidovirus infections in reptiles.
Path Tracking Control of Autonomous Vehicle Based on Nonlinear Tire Model
Authors: Lin Fen , Sun Minghong , Wu Jian , Qian Chengliang
Published: September 2021

The tire forces of vehicles will fall into the non-linear region under extreme handling conditions, which cause poor path tracking performance. In this paper, a model predictive controller based on a nonlinear tire model is designed. The tire forces are characterized with nonlinear composite functions of the magic formula instead of a simple linear relation model. Taylor expansion is used to linearize the controller, the first-order difference quotient method is used for discretization, and the partial derivative of the composite function is used for matrix transformation. Constant velocity and variable velocity conditions are selected to compare the designed controller with the conventional controller in Carsim/Simulink. The results show that when the tire forces fall in the nonlinear region, two controllers have good stability, and the tracking accuracy of the controller designed in this paper is slightly better. However, after the tire forces become nonlinear, the controller with linear tire force becomes worse, the tracking accuracy is far worse than the controller with the nonlinear tire model, and the vehicle stability is also degraded. In addition, an active steering test platform based on LabVIEW-RT is established, and hardware-in-the-loop tests are carried out. The effectiveness of the designed controller is verified.
Reversible Torsional Actuation of Hydrogel Filled Multifilament Fibre Actuator
Authors: Zhang Xi , Zhang Jinxuan , Salahuddin Bidita , Gao Shuai , Aziz Shazed , Zhu Zhonghua
Published: September 2021

Twisted polymer fibre actuators provide high torsional rotation from stimulated volume expansion, induced either by chemical fuelling, thermal stimulation, or electrochemical charging. One key limitation of these actuators is the irreversibility of torsional stroke that limits their feasibility when considering real-life smart applications. Moreover, scaling the torsional stroke of these actuators becomes difficult when these are integrated into practically usable systems such as smart textiles, due to the external and variable opposing torque that is applied by the adjacent non-actuating fibres. Herein, a simple composite type torsional actuator made of hydrogel coated commercial textile cotton multifilament fibre is demonstrated. This novel actuator is of high moisture responsiveness, given that hydrogels are capable of providing huge volume expansion and twisting the overall system can transform the volumetric expansion to fibre untwisting based torsional actuation. Theoretical treatment of torsional actuation is also demonstrated based on the change in torsional stiffness of dry and wet fibres as well as a few externally applied torques. The agreement between experimental measurements and theoretical estimation is found reasonable, and the investigation allows the near-appropriate estimation of torsional stroke before integrating an actuator into a smart system.
Micro-Vortex Generators on Transonic Convex-Corner Flow
Authors: Chung Kung-Ming , Su Kao-Chun , Chang Keh-Chin
Published: September 2021

A convex corner models the upper surface of a deflected flap and shock-induced boundary layer separation occurs at transonic speeds. This study uses micro-vortex generators (MVGs) for flow control. An array of MVGs (counter-rotating vane type, ramp type and co-rotating vane type) with a height of 20% of the thickness of the incoming boundary layer is installed upstream of a convex corner. The surface pressure distributions are similar regardless of the presence of MVGs. They show mild upstream expansion, a strong favorable pressure gradient near the corner’s apex and downstream compression. A corrugated surface oil flow pattern is observed in the presence of MVGs and there is an onset of compression moving downstream. The counter-rotating vane type MVGs produce a greater reduction in peak pressure fluctuations and the ramp type decreases the separation length. The presence of MVGs stabilizes the shock and shock oscillation is damped.
Effect of Banana Bunchy Top Virus on the Heat Shock Protein Genes of Pentalonia nigronervosa during Temperature Susceptibility and Its Effect on Virus Transmission
Authors: Chakraborty Swati , Barman Mritunjoy , Samanta Snigdha , Roy Moupiya , Tarafdar Jayanta
Published: September 2021

Acquisition of plant viruses is known to exert various effects on vectors’ developmental biology. Pentalonia nigronervosa is the only known vector of banana bunchy top virus (BBTV), which is an economically detrimental virus infecting banana cultivars all over the world. In the present study, the developmental biology of viruliferous (Vr) and non-viruliferous (NVr) aphids was compared, with a marked reduction noted in the lifespan of aphids upon acquisition of BBTV. Among all the environmental parameters temperature is an important determinant of an insect’s abundance and geographical distribution. Temperature susceptibility of P. nigronervosa was scrutinized by comparing the mortality percentage and differential expression pattern of three heat shock proteins (Hsps; Hsp40, Hsp70, and Hsp90) at the mRNA level between NVr and Vr aphids. After exposure to different temperature stress (5 °C, 15 °C, 38 °C and 25 °C as control) highest mortality of Vr aphids were recorded at 5 °C. Analysis of expression levels of Hsp genes using qPCR showed that both cold and heat shock treatment stimulated higher expression of the three Hsps at various rates in Vr than NVr aphids.. Finally, the effect of temperature stress on the BBTV titer level and their transmission by P.nigronervosa was determined by absolute quantification. The transmission efficiency along with the virus titer was found to be the lowest at 15 °C compared to 38 °C. Overall, our results provide a novel insight into the intricate interaction between aphid fitness and thermal stress concerning the acquisition and transmission of BBTV, which could be a roadmap for the future epidemiological control system.
Sound Splits as Influenced by Seed Size for Runner and Virginia Market Type Peanut Shelled on a Reciprocating Sheller
Authors: Anco Daniel J. , Balota Maria , Dunne Jeffrey C. , Brown Nino
Published: September 2021

The objective of this study was to examine peanut (Arachis hypogaea L.) kernel percent sound splits as a function of sound mature kernel seed size when shelled on a reciprocating sheller. Data were compiled from a total of 139 field experiments conducted in the Virginia-Carolina region and Georgia from 2005 to 2020. Runner and Virginia peanut market types were graded according to United States Department of Agriculture (USDA) standards using standard sheller screens with upper grid sizes corresponding to the red pan from the pre-sizer of 10.3 × 19.1 mm (26/64 × 3/4 ″) and 13.5 × 25.4 mm (34/64 × 1 ″) with minimum bar grid clearances of 8.7 (11/32 ″) and 12.7 mm (1/2 ″), respectively. A subset of runner market type samples was graded using the Virginia sheller screen. Grade data per market type and sheller screen was analyzed separately. Among runner market types shelled with the standard runner-type screen, percent sound splits increased linearly with increasing seed size at the logit rate of 1.16 per sound mature kernel g (p < 0.001). Sound splits for Virginia and runner market types shelled on the standard Virginia-type screen did not significantly vary by kernel size (p = 0.939 and 0.687, respectively). Extra-large kernels (proportion) for Virginia types linearly increased with seed size at 1.91 per sound mature kernel g (logit scale) (p < 0.001). Runner market types sized 75 to 91 g/100 sound mature kernels (605 to 500 seed/lb) were estimated to have a 50% probability of a 2.3 to 4.5% or greater increase in sound splits when shelled with the standard runner-type screen compared to runner-type seed sized 55 g/100 sound mature kernels (820 seed/lb), respectively, equivalent to a potential deduction increase of 1.8 to 4.4 USD /1000 kg. For both Virginia and runner market types, seed weight linearly increased with pod weight at 0.169 and 0.195 g/g (p < 0.001), respectively. Results from this study may be used as a reference to suggest runner-type seed sizes above which larger reciprocating sheller screen utilization in line with USDA grading practices is warranted to reduce mechanically induced sound splits during grading and subsequent economic deduction penalties for corresponding farmer stock peanut.
The Performance of Representative Asian Vegetables in Different Production Systems in Texas
Authors: Niu Genhua , Masabni Joseph , Hooks Triston , Leskovar Daniel , Jifon John
Published: September 2021

Demand for Asian vegetables is rising rapidly due to changing demographics and increasing consumer awareness of their health benefits. However, growers are not familiar with growing these “foreign” crops due to insufficient technical information regarding suitable cultivars for different regions, production schedules, disease and pest susceptibility, and postharvest management. The objective of this study was to conduct trials in different production systems and climate regions to demonstrate the potential of growing Asian vegetables in Texas. We conducted preliminary trials of nine leafy greens in the open field, high tunnel, and greenhouse (container and hydroponic production) to explore the suitability and potential for year-round production. We also conducted field trials for warm season crops in the open field in different climate zones. Results indicated that for cool season leafy greens, open field production has a limited growing season, high tunnel has the potential to extend the growing season, while greenhouse may provide year-round production using soilless substrate container culture or hydroponic system. For warm season crops, early planting is recommended for high yield. Additional research is warranted in different regions to test more species and cultivars and optimize the production system of high-performing cultivars to maximize production and profitability.
Jasmonic Acid in Plant Abiotic Stress Tolerance and Interaction with Abscisic Acid
Authors: Kim Huijin , Seomun Subhin , Yoon Youngdae , Jang Geupil
Published: September 2021

The phytohormone jasmonic acid (JA), a cyclopentane fatty acid, mediates plant responses to abiotic stresses. Abiotic stresses rapidly and dynamically affect JA metabolism and JA responses by upregulating the expression of genes involved in JA biosynthesis and signaling, indicating that JA has a crucial role in plant abiotic stress responses. The crucial role of JA has been demonstrated in many previous studies showing that JA response regulates various plant defense systems, such as removal of reactive oxygen species and accumulation of osmoprotectants. Furthermore, increasing evidence shows that plant tolerance to abiotic stresses is linked to the JA response, suggesting that abiotic stress tolerance can be improved by modulating JA responses. In this review, we briefly describe the JA biosynthetic and signaling pathways and summarize recent studies showing an essential role of JA in plant responses and tolerance to a variety of abiotic stresses, such as drought, cold, salt, and heavy metal stress. Additionally, we discuss JA crosstalk with another key stress hormone, abscisic acid, in plant abiotic stress responses.
Potential Application of Rhizobacteria Isolated from the Central Highland of Vietnam as an Effective Biocontrol Agent of Robusta Coffee Nematodes and as a Bio-Fertilizer
Authors: Nguyen Dai Nam , Wang San-Lang , Nguyen Anh Dzung , Doan Manh Dung , Tran Dinh Minh , Nguyen Thi Huyen , Ngo Van Anh , Doan Chien Thang , Tran Thi Ngoc , Do Van Chung , Nguyen Van Bon
Published: September 2021

Robusta coffee is a major commercial crop in the Central Highland of Vietnam with high economic and export value. However, this crop is adversely affected by various pathogens, particularly nematodes. This study aimed to screen active anti-nematode rhizobacterial strains for sustainable coffee production. Among more than 200 isolates, the isolate TUN03 demonstrated efficient biocontrol with nearly 100% mortality of J2 coffee nematodes Meloidogyne spp. and 84% inhibition of nematode egg hatching. This active strain was identified as Pseudomonas aeruginosa TUN03 based on its 16S rRNA gene sequence and phylogenetic analysis. In greenhouse tests, the strain TUN03 significantly reduced the coffee nematode population in the rhizome-soil with an 83.23% inhibition rate and showed plant growth-promoting effects. Notably, this is the first report of the nematicidal effect of P. aeruginosa against coffee nematodes. This potent strain further showed an antifungal effect against various crop-pathogenic fungi and was found to be the most effective against Fusarium solani F04 (isolated from coffee roots) with a 70.51% inhibition rate. In addition, high-performance liquid chromatography analysis revealed that this bacterial strain also secretes plant growth regulators including indole acetic acid (IAA), gibberellic acid (GA3), kinetin, and zeatin in significant amounts of 100, 2700, 37, and 9.5 µg/mL, respectively. The data from this study suggest that P. aeruginosa TUN03 may be a potential biocontrol agent and biofertilizer for the sustainable production of Robusta coffee and other crops.
RIANN—A Robust Neural Network Outperforms Attitude Estimation Filters
Authors: Weber Daniel , Gühmann Clemens , Seel Thomas
Published: September 2021

Inertial-sensor-based attitude estimation is a crucial technology in various applications, from human motion tracking to autonomous aerial and ground vehicles. Application scenarios differ in characteristics of the performed motion, presence of disturbances, and environmental conditions. Since state-of-the-art attitude estimators do not generalize well over these characteristics, their parameters must be tuned for the individual motion characteristics and circumstances. We propose RIANN, a ready-to-use, neural network-based, parameter-free, real-time-capable inertial attitude estimator, which generalizes well across different motion dynamics, environments, and sampling rates, without the need for application-specific adaptations. We gather six publicly available datasets of which we exploit two datasets for the method development and the training, and we use four datasets for evaluation of the trained estimator in three different test scenarios with varying practical relevance. Results show that RIANN outperforms state-of-the-art attitude estimation filters in the sense that it generalizes much better across a variety of motions and conditions in different applications, with different sensor hardware and different sampling frequencies. This is true even if the filters are tuned on each individual test dataset, whereas RIANN was trained on completely separate data and has never seen any of these test datasets. RIANN can be applied directly without adaptations or training and is therefore expected to enable plug-and-play solutions in numerous applications, especially when accuracy is crucial but no ground-truth data is available for tuning or when motion and disturbance characteristics are uncertain. We made RIANN publicly available.
Dietary Phospholipids Enhance Growth Performance and Modulate Cold Tolerance in Meagre (Argyrosomus regius) Juveniles
Authors: Hachero-Cruzado Ismael , Manchado Manuel
Published: September 2021

Meagre (Argyrosomus regius) is a fast-growing species currently produced in aquaculture. This species is highly sensitive to low environmental temperatures which results in high mortality events during production cycles. In this study, the effects of dietary phospholipids (PLs) on growth and cold tolerance were evaluated. For this purpose, control (CTRL) and PL-enriched diets (three-fold higher levels than CTRL) were supplied to meagre juveniles (12.9 ± 2.5 g) for 60 days, and growth was determined using a longitudinal approach. Weight gaining and SGR reduction were significantly different between dietary treatments. Animals fed with the PL-enriched diet were 4.1% heavier and grew 3.2% faster than those fed with the CTRL diet. Survival was higher than 98% in both groups. After finishing the growth trial, animals were submitted to two cold challenges and cold tolerance was evaluated as temperature at death (Tdeath), risk to death and lethal doses (LD) 50 and 90 using the cumulative degree cooling hours 6 h (CD6H). Tdeath ranged between 7.54 and 7.91 °C without statistical differences between dietary treatments. However, risk to death was significantly smaller (0.91-fold lower) and LD50 and LD90 were higher in animals fed with the PL-enriched than those supplied the CTRL diet. To assess the fatty acid (FA) composition of liver and brain in animals fed both diets after a cold challenge, FA profiles were determined in juveniles maintained at 14 °C and challenged at 7 °C. FA amounts increased in the liver of animals challenged at 7 °C. In contrast, several FAs reduced their levels in the PL-enriched diet with respect to CTRL indicating that these animals were able to mobilize efficiently lipids from this organ mitigating the negative effects of lipid accumulation during the cold challenge. In brain, the PL-enriched diet increased DHA level during the cold shock indicating a role in maintaining of brain functions. These results open a new research line that could improve the cold tolerance of meagre through dietary supplementation before winter.
LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model
Authors: Abdel-Salam Mostafa A. L. , Pinto Bárbara , Cassali Geovanni , Bueno Lilian , Pêgas Gabriela , Oliveira Fabrício , Silva Irismara , Klein André , Souza-Fagundes Elaine Maria de , de Lima Maria Elena , Carvalho-Tavares Juliana
Published: September 2021

Cationic anticancer peptides have exhibited potent anti-proliferative and anti-inflammatory effects in neoplastic illness conditions. LyeTx I-b is a synthetic peptide derived from Lycosa erythrognatha spider venom that previously showed antibiotic activity in vitro and in vivo. This study focused on the effects of LyeTxI-b on a 4T1 mouse mammary carcinoma model. Mice with a palpable tumor in the left flank were subcutaneously or intratumorally injected with LyeTx I-b (5 mg/kg), which significantly decreased the tumor volume and metastatic nodules. Histological analyses showed a large necrotic area in treated primary tumors compared to the control. LyeTxI-b reduced tumor growth and lung metastasis in the 4T1 mouse mammary carcinoma model with no signs of toxicity in healthy or cancerous mice. The mechanism of action of LyeTx I-b on the 4T1 mouse mammary carcinoma model was evaluated in vitro and is associated with induction of apoptosis and cell proliferation inhibition. Furthermore, LyeTx I-b seems to be an efficient regulator of the 4T1 tumor microenvironment by modulating several cytokines, such as TGF-β, TNF-α, IL-1β, IL-6, and IL-10, in primary tumor and lung, spleen, and brain. LyeTx I-b also plays a role in leukocytes rolling and adhesion into spinal cord microcirculation and in the number of circulating leukocytes. These data suggest a potent antineoplastic efficacy ofLyeTx I-b.
Iron Oxide–Silica Core–Shell Nanoparticles Functionalized with Essential Oils for Antimicrobial Therapies
Authors: Chircov Cristina , Matei Maria-Florentina , Neacșu Ionela Andreea , Vasile Bogdan Stefan , Oprea Ovidiu-Cristian , Croitoru Alexa-Maria , Trușcă Roxana-Doina , Andronescu Ecaterina , Sorescu Ionuț , Bărbuceanu Florica
Published: September 2021

Recent years have witnessed a tremendous interest in the use of essential oils in biomedical applications due to their intrinsic antimicrobial, antioxidant, and anticancer properties. However, their low aqueous solubility and high volatility compromise their maximum potential, thus requiring the development of efficient supports for their delivery. Hence, this manuscript focuses on developing nanostructured systems based on Fe3O4@SiO2 core–shell nanoparticles and three different types of essential oils, i.e., thyme, rosemary, and basil, to overcome these limitations. Specifically, this work represents a comparative study between co-precipitation and microwave-assisted hydrothermal methods for the synthesis of Fe3O4@SiO2 core–shell nanoparticles. All magnetic samples were characterized by X-ray diffraction (XRD), gas chromatography-mass spectrometry (GC-MS), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), zeta potential, scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetry and differential scanning calorimetry (TG-DSC), and vibrating sample magnetometry (VSM) to study the impact of the synthesis method on the nanoparticle formation and properties, in terms of crystallinity, purity, size, morphology, stability, and magnetization. Moreover, the antimicrobial properties of the synthesized nanocomposites were assessed through in vitro tests on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. In this manner, this study demonstrated the efficiency of the core–shell nanostructured systems as potential applications in antimicrobial therapies.
Delayed Rifampin Administration in the Antibiotic Treatment of Periprosthetic Joint Infections Significantly Reduces the Emergence of Rifampin Resistance
Authors: Darwich Ali , Dally Franz-Joseph , Bdeir Mohamad , Kehr Katharina , Miethke Thomas , Hetjens Svetlana , Gravius Sascha , Assaf Elio , Mohs Elisabeth
Published: September 2021

Rifampin is one of the most important biofilm-active antibiotics in the treatment of periprosthetic joint infection (PJI), and antibiotic regimens not involving rifampin were shown to have higher failure rates. Therefore, an emerging rifampin resistance can have a devastating effect on the outcome of PJI. The aim of this study was to compare the incidence of rifampin resistance between two groups of patients with a PJI treated with antibiotic regimens involving either immediate or delayed additional rifampin administration and to evaluate the effect of this resistance on the outcome. In this retrospective analysis of routinely collected data, all patients who presented with an acute/chronic PJI between 2018 and 2020 were recorded in the context of a single-center comparative cohort study. Two groups were formed: Group 1 included 25 patients with a PJI presenting in 2018–2019. These patients received additional rifampin only after pathogen detection in the intraoperative specimens. Group 2 included 37 patients presenting in 2019–2020. These patients were treated directly postoperatively with an empiric antibiotic therapy including rifampin. In all, 62 patients (32 females) with a mean age of 68 years and 322 operations were included. We found a rifampin-resistant organism in 16% of cases. Rifampin resistance increased significantly from 12% in Group 1 to 19% in Group 2 (p < 0.05). The treatment failure rate was 16% in Group 1 and 16.2% in Group 2 (p = 0.83). The most commonly isolated rifampin-resistant pathogen was Staphylococcus epidermidis (86%) (p < 0.05). The present study shows a significant association between the immediate start of rifampin after surgical revision in the treatment of PJI and the emergence of rifampin resistance, however with no significant effect on outcome.
Detection of Multidrug-Resistant Enterobacterales—From ESBLs to Carbapenemases
Authors: Noster Janina , Thelen Philipp , Hamprecht Axel
Published: September 2021

Multidrug-resistant Enterobacterales (MDRE) are an emerging threat to global health, leading to rising health care costs, morbidity and mortality. Multidrug-resistance is commonly caused by different β-lactamases (e.g., ESBLs and carbapenemases), sometimes in combination with other resistance mechanisms (e.g., porin loss, efflux). The continuous spread of MDRE among patients in hospital settings and the healthy population require adjustments in healthcare management and routine diagnostics. Rapid and reliable detection of MDRE infections as well as gastrointestinal colonization is key to guide therapy and infection control measures. However, proper implementation of these strategies requires diagnostic methods with short time-to-result, high sensitivity and specificity. Therefore, research on new techniques and improvement of already established protocols is inevitable. In this review, current methods for detection of MDRE are summarized with focus on culture based and molecular techniques, which are useful for the clinical microbiology laboratory.
Peroxisomal PEX7 Receptor Affects Cadmium-Induced ROS and Auxin Homeostasis in Arabidopsis Root System
Authors: Piacentini Diego , Della Rovere Federica , Bertoldi Ilaria , Massimi Lorenzo , Sofo Adriano , Altamura Maria Maddalena , Falasca Giuseppina
Published: September 2021

Peroxisomes are important in plant physiological functions and stress responses. Through the production of reactive oxygen and nitrogen species (ROS and RNS), and antioxidant defense enzymes, peroxisomes control cellular redox homeostasis. Peroxin (PEX) proteins, such as PEX7 and PEX5, recognize peroxisome targeting signals (PTS1/PTS2) important for transporting proteins from cytosol to peroxisomal matrix. pex7-1 mutant displays reduced PTS2 protein import and altered peroxisomal metabolism. In this research we analyzed the role of PEX7 in the Arabidopsis thaliana root system exposed to 30 or 60 μM CdSO4. Cd uptake and translocation, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) levels, and reactive oxygen species (ROS) and reactive nitrogen species (RNS) levels and catalase activity were analyzed in pex7-1 mutant primary and lateral roots in comparison with the wild type (wt). The peroxisomal defect due to PEX7 mutation did not reduce Cd-uptake but reduced its translocation to the shoot and the root cell peroxisomal signal detected by 8-(4-Nitrophenyl) Bodipy (N-BODIPY) probe. The trend of nitric oxide (NO) and peroxynitrite in pex7-1 roots, exposed/not exposed to Cd, was as in wt, with the higher Cd-concentration inducing higher levels of these RNS. By contrast, PEX7 mutation caused changes in Cd-induced hydrogen peroxide (H2O2) and superoxide anion (O2●−) levels in the roots, delaying ROS-scavenging. Results show that PEX7 is involved in counteracting Cd toxicity in Arabidopsis root system by controlling ROS metabolism and affecting auxin levels. These results add further information to the important role of peroxisomes in plant responses to Cd.
Employment of Flake Ice Systems Including Natural Preservative Compounds for the Quality Enhancement of Chilled Seafood—A Review
Authors: Aubourg Santiago P.
Published: September 2021

Marine species deteriorate rapidly post-mortem as a consequence of a variety of biochemical and microbial breakdown mechanisms. Due to the increasing demand for high-quality fresh seafood, different strategies are now available to retard spoilage for as long as possible. The present study provides an overview of a recently proposed strategy based on the addition of natural compounds to marine species. In this strategy, different kinds of natural preservative compounds are included in the flake-ice medium that is commonly used for chilled storage. Natural sources tested for this purpose include low-molecular-weight organic acids and different kinds of extracts of plants, macroalgae, and by-products resulting from marine species commercialization. The preservative action of such treatments is analyzed according to the effect on different deteriorative mechanisms (i.e., lipid hydrolysis, oxidation, and microbial activity development), as well as on the resulting sensory acceptability and shelf-life time. The basic objective of this review is to provide an overview concerning the positive effect that the presence in an icing system of natural preserving compounds may have on the quality of chilled marine species. Furthermore, various potential avenues are proposed to develop the practical and commercial employment of this technological strategy.
Scheduling Period Selection Based on Stability Analysis for Engagement Control Task of Automatic Clutches
Authors: Ding Zhao , Chen Li , Chen Jun , Cheng Xiaoxuan , Yin Chengliang
Published: September 2021

The clutch engagement process involves three phases known as open, slipping, and locked and takes a few seconds. The engagement control program runs in an embedded control unit, in which discretization may induce oscillation and even instability in the powertrain due to an improper scheduling period for the engagement control task. To properly select the scheduling period, a methodology for control–scheduling co-design during clutch engagement is proposed. Considering the transition of the friction state from slipping to being locked, the co-design framework consists of two steps. In the first step, a stability analysis is conducted for the slipping phase based on a linearized system model enveloping the driving and driven part of the clutch, feed-forward and feedback control loop together with a zero-order signal hold element. The critical period is determined according to pole locations, and factors influencing the critical period are investigated. In the second step, real-time hardware-in-the-loop experiments are carried out to inspect the dynamic response concerning the friction state transition. A sub-boundary within the stable region is found to guarantee the control performance to satisfy the engineering requirements. In general, the vehicle jerk and clutch frictional loss increase with the increase in the scheduling period. When the scheduling period is shorter than the critical period, the rate of increase is mild. However, once the scheduling period exceeds the critical period, the rate of increase becomes very high.
Graffiti Characterization Prior to Intervention in the Punta Begoña Galleries (Getxo, North of Spain): Raman and XRF Spectroscopy in the Service of Restoration
Authors: Etxebarria Idoia , Prieto-Taboada Nagore , Lama Estibaliz , Arana Gorka , Rodríguez-Laso María Dolores , Madariaga Juan Manuel
Published: September 2021

The Historical and Cultural Heritage of Punta Begoña Galleries in Getxo (Bizkaia, North of Spain) are currently in restoration after being abandoned for years. For that reason, many graffiti, which directly affect the wall paintings, appear on most of their walls. Moreover, several graffiti overlap each other, which makes their removal more difficult. For all these reasons, the chemical characterization of these pigments is a priority to optimize the cleaning and consolidation treatments of wall paintings. That being the case, an analysis based on Raman spectroscopy and X-ray fluorescence was carried out to obtain information to help conservators remove the graffiti without damaging the mural paintings and the support. Nevertheless, the first step, using X-ray fluorescence and Raman spectroscopy, involved the need for a database to compare the results and identify the compounds. Thus, different commercial inks were elementally and molecularly characterized to complete the existing databases. After this, an analysis of the inks was carried out that noted the presence of several organic pigments, such as phthalocyanines. Inorganic pigments such as titanium oxide were identified as well. After the analysis, the selection of the best removal process could be carried out to provide the most effective treatment, avoiding the “trial-and-error” classical practice.
Solid-State Transformation of an Additive Manufactured Inconel 625 Alloy at 700 °C
Authors: Zhang Fan , Ilavsky Jan , Lindwall Greta , Stoudt Mark R. , Levine Lyle E. , Allen Andrew J.
Published: September 2021

Inconel 625, a nickel-based superalloy, has drawn much attention in the emerging field of additive manufacturing (AM) because of its excellent weldability and resistance to hot cracking. The extreme processing condition of AM often introduces enormous residual stress (hundreds of MPa to GPa) in the as-fabricated parts, which requires stress-relief heat treatment to remove or reduce the internal stresses. Typical residual stress heat treatment for AM Inconel 625, conducted at 800 °C or 870 °C, introduces a substantial precipitation of the δ phase, a deleterious intermetallic phase. In this work, we used synchrotron-based in situ scattering and diffraction methods and ex situ electron microscopy to investigate the solid-state transformation of an AM Inconel 625 at 700 °C. Our results show that while the δ phase still precipitates from the matrix at this temperature, its precipitation rate and size at a given time are both smaller when compared with their counterparts during typical heat treatment temperatures of 800 °C and 870 °C. A comparison with thermodynamic modeling predictions elucidates these experimental findings. Our work provides the rigorous microstructural kinetics data required to explore the feasibility of a promising lower-temperature stress-relief heat treatment for AM Inconel 625. The combined methodology is readily extendable to investigate the solid-state transformation of other AM alloys.
A Multicast Routing Scheme for the Internet: Simulation and Experimentation in Large-Scale Networks
Authors: Careglio Davide , Agraz Fernando , Papadimitriou Dimitri
Published: September 2021

With the globalisation of the multimedia entertainment industry and the popularity of streaming and content services, multicast routing is (re-)gaining interest as a bandwidth saving technique. In the 1990’s, multicast routing received a great deal of attention from the research community; nevertheless, its main problems still remain mostly unaddressed and do not reach the acceptance level required for its wide deployment. Among other reasons, the scaling limitation and the relative complexity of the standard multicast protocol architecture can be attributed to the conventional approach of overlaying the multicast routing on top of the unicast routing topology. In this paper, we present the Greedy Compact Multicast Routing (GCMR) scheme. GMCR is characterised by its scalable architecture and independence from any addressing and unicast routing schemes; more specifically, the local knowledge of the cost to direct neighbour nodes is enough for the GCMR scheme to properly operate. The branches of the multicast tree are constructed directly by the joining destination nodes which acquire the routing information needed to reach the multicast source by means of an incremental two-stage search process. In this paper we present the details of GCMR and evaluate its performance in terms of multicast tree size (i.e., the stretch), the memory space consumption, the communication cost, and the transmission cost. The comparative performance analysis is performed against one reference algorithm and two well-known protocol standards. Both simulation and emulation results show that GCMR achieves the expected performance objectives and provide the guidelines for further improvements.
Shifting from Conventional to Organic Filter Media in Wastewater Biofiltration Treatment: A Review
Authors: Loh Zhang Zhan , Zaidi Nur Syamimi , Syafiuddin Achmad , Yong Ee Ling , Boopathy Raj , Hong Kueh Ahmad Beng , Prastyo Dedy Dwi
Published: September 2021

Biofiltration is a promising wastewater treatment green technology employed to remove various types of pollutants. The efficiency of biofiltration relies on biofilm, and its performance is significantly influenced by various factors such as dissolved oxygen concentration, organic loading rate, hydraulic retention time, temperature, and filter media selection. The existing biofilters utilize conventional media such as gravel, sand, anthracite, and many other composite materials. The material cost of these conventional filter materials is usually higher compared to using organic waste materials as the filter media. However, the utilization of organic materials as biofilter media has not been fully explored and their potential in terms of physicochemical properties to promote biofilm growth is lacking in the literature. Therefore, this review critically discusses the potential of shifting conventional filter media to that of organic in biofiltration wastewater treatment, focusing on filtration efficiency-influenced factors, their comparative filtration performance, advantages, and disadvantages, as well as challenges and prospective areas of organic biofilter development.
One- and Two-Step Kinetic Data Analysis Applied for Single and Co-Culture Growth of Staphylococcus aureus, Escherichia coli, and Lactic Acid Bacteria in Milk
Authors: Ačai Pavel , Valík Ľubomír , Medveďová Alžbeta
Published: September 2021

The objective of this study was to compare one- and two-step kinetic data analysis approaches to describe the growth of Staphylococcus aureus, Escherichia coli, and lactic acid bacteria Fresco 1010 starter culture in milk under isothermal conditions between 10 and 37 °C. The primary Huang model (HM) and secondary square root model were applied to lag times and growth rates of each of the population. The one-step approach for single cultures data enabled the direct construction of a tertiary model combining primary and secondary models to determine parameters from all growth data, thus minimizing the transfer of errors from one model to another. The statistical indices showed a significant improvement in the prediction capability provided by this approach. Then, a one-step approach combining the primary Huang, Giménez, and Dalgaard model (H-GD) with the secondary square root model was used to simultaneously model the growth of the populations mentioned above in co-culture under the same conditions. Independent isothermal data sets were chosen for validation of the growth description of single cultures (HM) and co-culture (H-GD) using validation factors, including the bias (Bf) and accuracy (Af). For example, the values of Af for the one-step approach range from 1.17 to 1.20 and 1.04 to 1.08 for single cultures and co-culture, respectively, demonstrating high accuracy. Thus, this approach may be used for co-culture growth description in general or specifically, e.g., in various types of lactic acid fermentation, including artisanal cheese-making technology.
A Review of the Methods Calculating the Horizontal Displacement for Modular Reinforced Soil Retaining Walls
Authors: Cai Xiaoguang , Zhang Shaoqiu , Li Sihan , Xu Honglu , Huang Xin , Zhu Chen
Published: September 2021

Most of the damage to reinforced retaining walls is caused by excessive deformation; however, there is no calculation method for deformation under static and dynamic loads in the design codes of reinforced soil retaining walls. In this paper, by collecting the measured displacement data from four actual projects, four indoor prototype tests and two indoor model tests under a total of 10 static load conditions, and comparing the calculation results with seven theoretical methods, the results show that the FHWA method is more applicable to the permanent displacement prediction of indoor prototype tests and that the CTI method is more applicable to the permanent displacement prediction of actual projects and indoor model tests. Two yield acceleration calculation methods and four permanent displacement calculation formulas were selected to calculate the displacement response of two reinforced soil test models under seismic loads and compared with the measured values, and the results showed that the Ausilio yield acceleration solution method was better. When the input peak acceleration ranges from 0.1 to 0.6 g, the Richards and Elms upper limit method is used, and when the input peak acceleration is 0.6–1.0 g, the Newmark upper limit method can predict the permanent displacement of the retaining wall more accurately.
Performance Evaluation of a Compound Power-Split CVT for Hybrid Powertrains
Authors: Mantriota Giacomo , Reina Giulio , Ugenti Angelo
Published: September 2021

The Power-Split Continuously Variable Transmission is one of the most promising architectures for Hybrid Electric Vehicles. These systems have been introduced to improve vehicle global efficiency since they can maximize the efficiency in varying operating conditions. During the design stage, the availability of modeling tools would play a key role in achieving optimal design and control of these architectures. In this work, a compound power split device that combines an electric Continuously Variable Transmission with two planetary gear trains is analyzed. A comprehensive model is derived that allows the different power flow configurations to be evaluated given the properties of the single subcomponents of the system. The efficiency of the powertrain can be derived as well, and a numerical example is provided. The architecture studied has an efficiency that can be higher than that obtained using one single eCVT for most of the global transmission ratio range, showing that this solution could be suitable as a part of a more complex compound transmission that engages in a specific speed range.