bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors
Authors: Kurihara Taisei , Suehara Yoshiyuki , Akaike Keisuke , Hayashi Takuo , Kohsaka Shinji , Ueno Toshihide , Hasegawa Nobuhiko , Takagi Tatsuya , Sasa Keita , Okubo Taketo , Kim Youngji , Mano Hiroyuki , Yao Takashi , Kaneko Kazuo , Saito Tsuyoshi
Published: October 2020

Gene expression imbalances were measured for tyrosine kinase (TK) genes using Nanostring in 19 samples of inflammatory myofibroblastic tumor (IMT). All cases were immunohistochemically stained with anaplastic lymphoma kinase (ALK) and pan-tropomyosin-related-kinase (pan-Trk) antibodies. Five cases with imbalanced ALK expression, reported with Nanostring, were tested using fluorescence in situ hybridization (FISH); two cases with imbalanced neurotrophic tyrosine receptor kinase 3 (NTRK3) expression were tested using reverse transcription-polymerase chain reaction (RT-PCR). One case with imbalanced expression for ROS proto-oncogene 1 (ROS1) was tested using RNA sequencing and RT-PCR. TK fusions were detected in all cases with imbalanced TK expression. RNA sequencing detected a FN1–ROS1 fusion gene in an adult IMT case. IMT with ALK rearrangement showed myofibroblast-dominant features. IMT with ETV6–NTRK3 fusion showed prominent lymphoplasmacytic infiltration with scattered myofibroblasts. Pan-Trk IHC revealed only scattered positively stained cells in IMT with ETV6–NTRK3 fusion gene. ROS1-positive IMT showed myofibroblast-dominant features.
Whole transcriptome analysis of adrenal glands from prenatal glucocorticoid programmed hypertensive rodents
Authors: Tharmalingam Sujeenthar , Khurana Sandhya , Murray Alyssa , Lamothe Jeremy , Tai T. C.
Published: October 2020

Prenatal glucocorticoid exposure is associated with the development of hypertension in adults. We have previously demonstrated that antenatal dexamethosone (DEX) administration in Wistar-Kyoto dams results in offspring with increased blood pressure coupled with elevated plasma epinephrine levels. In order to elucidate the molecular mechanisms responsible for prenatal DEX-mediated programming of hypertension, a whole-transcriptome analysis was performed on DEX programmed WKY male adrenal glands using the Rat Gene 2.0 microarray. Differential gene expression (DEG) analysis of DEX-exposed offspring compared with saline-treated controls revealed 142 significant DEGs (109 upregulated and 33 downregulated genes). DEG pathway enrichment analysis demonstrated that genes involved in circadian rhythm signaling were most robustly dysregulated. RT-qPCR analysis confirmed the increased expression of circadian genes Bmal1 and Npas2, while Per2, Per3, Cry2 and Bhlhe41 were significantly downregulated. In contrast, gene expression profiling of Spontaneously Hypertensive (SHR) rats, a genetic model of hypertension, demonstrated decreased expression of Bmal1 and Npas2, while Per1, Per2, Per3, Cry1, Cry2, Bhlhe41 and Csnk1D were all upregulated compared to naïve WKY controls. Taken together, this study establishes that glucocorticoid programmed adrenals have impaired circadian signaling and that changes in adrenal circadian rhythm may be an underlying molecular mechanism responsible for the development of hypertension.
Transcapillary transport of water, small solutes and proteins during hemodialysis
Authors: Pstras Leszek , Waniewski Jacek , Lindholm Bengt
Published: October 2020

The semipermeable capillary walls not only enable the removal of excess body water and solutes during hemodialysis (HD) but also provide an essential mechanism for maintaining cardiovascular homeostasis. Here, we investigated transcapillary transport processes on the whole-body level using the three-pore model of the capillary endothelium with large, small and ultrasmall pores. The transcapillary transport and cardiovascular response to a 4-h hemodialysis (HD) with 2 L ultrafiltration were analyzed by simulations in a virtual patient using the three-pore model of the capillary wall integrated in the whole-body compartmental model of the cardiovascular system with baroreflex mechanisms. The three-pore model revealed substantial changes during HD in the magnitude and direction of transcapillary water flows through small and ultrasmall pores and associated changes in the transcapillary convective transport of proteins and small solutes. The fraction of total capillary hydraulic conductivity attributed to ultrasmall pores was found to play an important role in the transcapillary water transport during HD thus influencing the cardiovascular response to HD. The presented model provides a novel computational framework for a detailed analysis of microvascular exchange during HD and as such may contribute to a better understanding of dialysis-induced changes in blood volume and blood pressure.
Publisher Correction: Groundwater Throughflow and Seawater Intrusion in High Quality Coastal Aquifers
Authors: Costall A. R. , Harris B. D. , Teo B. , Schaa R. , Wagner F. M. , Pigois J. P.
Published: October 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Mild electrical stimulation with heat shock attenuates renal pathology in adriamycin-induced nephrotic syndrome mouse model
Authors: Teramoto Keisuke , Tsurekawa Yu , Suico Mary Ann , Kaseda Shota , Omachi Kohei , Yokota Tsubasa , Kamura Misato , Piruzyan Mariam , Kondo Tatsuya , Shuto Tsuyoshi , Araki Eiichi , Kai Hirofumi
Published: October 2020

Nephrotic syndrome (NS) is a renal disorder that is characterized by massive proteinuria, hypoalbuminemia and edema. One of the main causes of NS is focal segmental glomerulosclerosis (FSGS), which has extremely poor prognosis. Although steroids and immunosuppressants are the first line of treatment, some FSGS cases are refractory, prompting the need to find new therapeutic strategies. We have previously demonstrated that an optimized combination treatment of mild electrical stimulation (MES) and heat shock (HS) has several biological benefits including the amelioration of the pathologies of the genetic renal disorder Alport syndrome. Here, we investigated the effect of MES + HS on adriamycin (ADR)-induced NS mouse model. MES + HS suppressed proteinuria and glomerulosclerosis induced by ADR. The expressions of pro-inflammatory cytokines and pro-fibrotic genes were also significantly downregulated by MES + HS. MES + HS decreased the expression level of cleaved caspase-3 and the number of TUNEL-positive cells, indicating that MES + HS exerted anti-apoptotic effect. Moreover, MES + HS activated the Akt signaling and induced the phosphorylation and inhibition of the apoptotic molecule BAD. In in vitro experiment, the Akt inhibitor abolished the MES + HS-induced Akt-BAD signaling and anti-apoptotic effect in ADR-treated cells. Collectively, our study suggested that MES + HS modulates ADR-induced pathologies and has renoprotective effect against ADR-induced NS via regulation of Akt-BAD axis.
Machine learning prediction for mortality of patients diagnosed with COVID-19: a nationwide Korean cohort study
Authors: An Chansik , Lim Hyunsun , Kim Dong-Wook , Chang Jung Hyun , Choi Yoon Jung , Kim Seong Woo
Published: October 2020

The rapid spread of COVID-19 has resulted in the shortage of medical resources, which necessitates accurate prognosis prediction to triage patients effectively. This study used the nationwide cohort of South Korea to develop a machine learning model to predict prognosis based on sociodemographic and medical information. Of 10,237 COVID-19 patients, 228 (2.2%) died, 7772 (75.9%) recovered, and 2237 (21.9%) were still in isolation or being treated at the last follow-up (April 16, 2020). The Cox proportional hazards regression analysis revealed that age > 70, male sex, moderate or severe disability, the presence of symptoms, nursing home residence, and comorbidities of diabetes mellitus (DM), chronic lung disease, or asthma were significantly associated with increased risk of mortality (p ≤ 0.047). For machine learning, the least absolute shrinkage and selection operator (LASSO), linear support vector machine (SVM), SVM with radial basis function kernel, random forest (RF), and k-nearest neighbors were tested. In prediction of mortality, LASSO and linear SVM demonstrated high sensitivities (90.7% [95% confidence interval: 83.3, 97.3] and 92.0% [85.9, 98.1], respectively) and specificities (91.4% [90.3, 92.5] and 91.8%, [90.7, 92.9], respectively) while maintaining high specificities > 90%, as well as high area under the receiver operating characteristics curves (0.963 [0.946, 0.979] and 0.962 [0.945, 0.979], respectively). The most significant predictors for LASSO included old age and preexisting DM or cancer; for RF they were old age, infection route (cluster infection or infection from personal contact), and underlying hypertension. The proposed prediction model may be helpful for the quick triage of patients without having to wait for the results of additional tests such as laboratory or radiologic studies, during a pandemic when limited medical resources must be wisely allocated without hesitation.
Nearshore neonate dispersal of Atlantic leatherback turtles (Dermochelys coriacea) from a non-recovering subpopulation
Authors: Hoover Aimee L. , Shillinger George L. , Williamson Sean A. , Reina Richard D. , Bailey Helen
Published: October 2020

The cryptic ‘lost years’ of sea turtles challenge conservation efforts due to unknown movements and habitat utilisation of young life stages. Behavioural information strengthens dispersal and habitat utilisation models estimating unidentified movements. In this study, leatherback hatchlings were actively tracked with miniature acoustic tags off the east coast of Costa Rica for 83.15 min (± 9.12 SD) to determine their movements and swimming behaviour. Drifters were deployed throughout the tracking process to obtain surface current data. Hatchling (n = 42) over-ground and in-water swimming speed and bearing were calculated. Mean over-ground distance travelled was 2.03 km (± 0.71 km SD) with an over-ground average swim speed of 0.41 m/s (± 0.15 m/s SD). Mean bearing was 108.08° (± 20.19° SD) compared to the 137.56° (± 44.00° SD) bearing of nearshore ocean currents during tracking. Hatchling mean in-water swimming speed was 0.25 m/s (± 0.09 m/s SD). The lower in-water speed suggests hatchlings were advected by the currents, with overall movement strongly influenced by the current direction. This information can be assimilated into broader spatiotemporal distribution models to interpret the influence of directional swimming on ecosystem utilisation and help to achieve informed management decisions across all life stages of the population.
Molecular specializations of deep cortical layer analogs in songbirds
Authors: Nevue Alexander A. , Lovell Peter V. , Wirthlin Morgan , Mello Claudio V.
Published: October 2020

How the evolution of complex behavioral traits is associated with the emergence of novel brain pathways is largely unknown. Songbirds, like humans, learn vocalizations via tutor imitation and possess a specialized brain circuitry to support this behavior. In a comprehensive in situ hybridization effort, we show that the zebra finch vocal robust nucleus of the arcopallium (RA) shares numerous markers (e.g. SNCA, PVALB) with the adjacent dorsal intermediate arcopallium (AId), an avian analog of mammalian deep cortical layers with involvement in motor function. We also identify markers truly unique to RA and thus likely linked to modulation of vocal motor function (e.g. KCNC1, GABRE), including a subset of the known shared markers between RA and human laryngeal motor cortex (e.g. SLIT1, RTN4R, LINGO1, PLXNC1). The data provide novel insights into molecular features unique to vocal learning circuits, and lend support for the motor theory for vocal learning origin.
Author Correction: Non-Invasive Photoacoustic Imaging of In Vivo Mice with Erythrocyte Derived Optical Nanoparticles to Detect CAD/MI
Authors: Liu Yonggang , Hanley Taylor , Chen Hao , Long Steven R. , Gambhir Sanjiv S. , Cheng Zhen , Wu Joseph C. , Fakhri Georges El , Anvari Bahman , Zaman Raiyan T.
Published: October 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Plasmopara viticola infection affects mineral elements allocation and distribution in Vitis vinifera leaves
Authors: Cesco Stefano , Tolotti Anna , Nadalini Stefano , Rizzi Stefano , Valentinuzzi Fabio , Mimmo Tanja , Porfido Carlo , Allegretta Ignazio , Giovannini Oscar , Perazzolli Michele , Cipriani Guido , Terzano Roberto , Pertot Ilaria , Pii Youry
Published: October 2020

Plasmopara viticolais one of the most important pathogens infectingVitis viniferaplants. The interactions amongP. viticolaand both susceptible and resistant grapevine plants have been extensively characterised, at transcriptomic, proteomic and metabolomic levels. However, the involvement of plants ionome in the response against the pathogen has been completely neglected so far. Therefore, this study was aimed at investigating the possible role of leaf ionomic modulation during compatible and incompatible interactions betweenP. viticolaand grapevine plants. In susceptible cultivars, a dramatic redistribution of mineral elements has been observed, thus uncovering a possible role for mineral nutrients in the response against pathogens. On the contrary, the resistant cultivars did not present substantial rearrangement of mineral elements at leaf level, except for manganese (Mn) and iron (Fe). This might demonstrate that, resistant cultivars, albeit expressing the resistance gene, still exploit a pathogen response mechanism based on the local increase in the concentration of microelements, which are involved in the synthesis of secondary metabolites and reactive oxygen species. Moreover, these data also highlight the link between the mineral nutrition and plants’ response to pathogens, further stressing that appropriate fertilization strategies can be fundamental for the expression of response mechanisms against pathogens.
Retrograde signaling by a mtDNA-encoded non-coding RNA preserves mitochondrial bioenergetics
Authors: Blumental-Perry A. , Jobava R. , Bederman I. , Degar A. J. , Kenche H. , Guan B. J. , Pandit K. , Perry N. A. , Molyneaux N. D. , Wu J. , Prendergas E. , Ye Z.-W. , Zhang J. , Nelson C. E. , Ahangari F. , Krokowski D. , Guttentag S. H. , Linden P. A. , Townsend D. M. , Miron A. , Kang M.-J. , Kaminski N. , Perry Y. , Hatzoglou M.
Published: October 2020

Alveolar epithelial type II (AETII) cells are important for lung epithelium maintenance and function. We demonstrate that AETII cells from mouse lungs exposed to cigarette smoke (CS) increase the levels of the mitochondria-encoded non-coding RNA, mito-RNA-805, generated by the control region of the mitochondrial genome. The protective effects of mito-ncR-805 are associated with positive regulation of mitochondrial energy metabolism, and respiration. Levels of mito-ncR-805 do not relate to steady-state transcription or replication of the mitochondrial genome. Instead, CS-exposure causes the redistribution of mito-ncR-805 from mitochondria to the nucleus, which correlated with the increased expression of nuclear-encoded genes involved in mitochondrial function. These studies reveal an unrecognized mitochondria stress associated retrograde signaling, and put forward the idea that mito-ncRNA-805 represents a subtype of small non coding RNAs that are regulated in a tissue- or cell-type specific manner to protect cells under physiological stress.
Cellular-resolution mapping uncovers spatial adaptive filtering at the rat cerebellum input stage
Authors: Casali Stefano , Tognolina Marialuisa , Gandolfi Daniela , Mapelli Jonathan , D’Angelo Egidio
Published: October 2020

Long-term synaptic plasticity is thought to provide the substrate for adaptive computation in brain circuits but very little is known about its spatiotemporal organization. Here, we combined multi-spot two-photon laser microscopy in rat cerebellar slices with realistic modeling to map the distribution of plasticity in multi-neuronal units of the cerebellar granular layer. The units, composed by ~300 neurons activated by ~50 mossy fiber glomeruli, showed long-term potentiation concentrated in the core and long-term depression in the periphery. This plasticity was effectively accounted for by an NMDA receptor and calcium-dependent induction rule and was regulated by the inhibitory Golgi cell loops. Long-term synaptic plasticity created effective spatial filters tuning the time-delay and gain of spike retransmission at the cerebellum input stage and provided a plausible basis for the spatiotemporal recoding of input spike patterns anticipated by the motor learning theory.
Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT)
Authors: Lee Nan-Ee , Kim Sun Hee , Yu Dae-Yeul , Woo Eui-Jeon , Kim Myung-Il , Seong Gi-Sang , Lee Sun Min , Ko Jeong-Heon , Kim Yong-Sam
Published: October 2020

Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use. Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements. Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods. Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice. Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability. Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics. Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
Authors: Shi Xiangyan , Prasanna Chinmayi , Soman Aghil , Pervushin Konstantin , Nordenskiöld Lars
Published: October 2020

The dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are formed by the residues exhibiting larger-scale μs-ms motions that extend from the NCP core to the histone tails and DNA. Furthermore, despite possessing highly conserved structural features, histones in the telomeric NCP exhibit enhanced μs-ms dynamics in the globular sites residing at the identified dynamic networks and in a neighboring region. In addition, higher mobility was observed for the N-terminal tails of hH3 and hH4 in the telomeric NCP. The results demonstrate the existence of dynamic networks in nucleosomes, through which the center of the core regions could interactively communicate with histone tails and DNA to potentially propagate epigenetic changes.
Evaluating inositol phospholipid interactions with inward rectifier potassium channels and characterising their role in disease
Authors: Pipatpolkai Tanadet , Corey Robin A. , Proks Peter , Ashcroft Frances M. , Stansfeld Phillip J.
Published: October 2020

Membrane proteins are frequently modulated by specific protein-lipid interactions. The activation of human inward rectifying potassium (hKir) channels by phosphoinositides (PI) has been well characterised. Here, we apply a coarse-grained molecular dynamics free-energy perturbation (CG-FEP) protocol to capture the energetics of binding of PI lipids to hKir channels. By using either a single- or multi-step approach, we establish a consistent value for the binding of PIP2 to hKir channels, relative to the binding of the bulk phosphatidylcholine phospholipid. Furthermore, by perturbing amino acid side chains on hKir6.2, we show that the neonatal diabetes mutation E179K increases PIP2 affinity, while the congenital hyperinsulinism mutation K67N results in a reduced affinity. We show good agreement with electrophysiological data where E179K exhibits a reduction in neomycin sensitivity, implying that PIP2 binds more tightly E179K channels. This illustrates the application of CG-FEP to compare affinities between lipid species, and for annotating amino acid residues.
Hydrogen release from a single water molecule on Vn+ (3 ≤ n ≤ 30)
Authors: Zhang Hanyu , Wu Haiming , Jia Yuhan , Yin Baoqi , Geng Lijun , Luo Zhixun , Hansen Klavs
Published: October 2020

Water and its interactions with metals are closely bound up with human life, and the reactivity of metal clusters with water is of fundamental importance for the understanding of hydrogen generation. Here a prominent hydrogen evolution reaction (HER) of single water molecule on vanadium clusters Vn+ (3 ≤ n ≤ 30) is observed in the reaction of cationic vanadium clusters with water at room temperature. The combined experimental and theoretical studies reveal that the wagging vibrations of a V-OH group give rise to readily formed V-O-V intermediate states on Vn+ (n ≥ 3) clusters and allow the terminal hydrogen to interact with an adsorbed hydrogen atom, enabling hydrogen release. The presence of three metal atoms reduces the energy barrier of the rate-determining step, giving rise to an effective production of hydrogen from single water molecules. This mechanism differs from dissociative chemisorption of multiple water molecules on aluminium cluster anions, which usually proceeds by dissociative chemisorption of at least two water molecules at multiple surface sites followed by a recombination of the adsorbed hydrogen atoms.
Long-distance coupling and energy transfer between exciton states in magnetically controlled microcavities
Authors: Ściesiek Maciej , Sawicki Krzysztof , Pacuski Wojciech , Sobczak Kamil , Kazimierczuk Tomasz , Golnik Andrzej , Suffczyński Jan
Published: October 2020

Coupling of quantum emitters in a semiconductor relies, generally, on short-range dipole-dipole or electronic exchange type interactions. Consistently, energy transfer between exciton states, that is, electron-hole pairs bound by Coulomb interaction, is limited to distances of the order of 10 nm. Here, we demonstrate polariton-mediated coupling and energy transfer between excitonic states over a distance exceeding 2 μm. We accomplish this by coupling quantum well-confined excitons through the delocalized mode of two coupled optical microcavities. Use of magnetically doped quantum wells enables us to tune the confined exciton energy by the magnetic field and in this way to control the spatial direction of the transfer. Such controlled, long-distance interaction between coherently coupled quantum emitters opens possibilities of a scalable implementation of quantum networks and quantum simulators based on solid-state, multi-cavity systems.
European Union Controls on Food Additives – A Historical Overview
Authors: Jukes David
Published: October 2020

The creation of the European Economic Community in 1959 led the initial six member states to tackle barriers to trade. The different national controls on food additives was one such barrier and the adoption of a Directive on colouring matters in 1962 was the first step in harmonisation. It was, however, to take until 2008 for a fully comprehensive system to be established. This chapter starts with a summary of the components of the European Union legislative framework. It then explains how the legislative controls were developed from the initial harmonisation programmes, through the development of the internal market and, finally, with the shift in emphasis from trade to public health, to the adoption of the current regulations.
Food Additive Regulations in Europe
Authors: Saltmarsh Mike
Published: October 2020

The use of all food additives in Europe is controlled by three Regulations: Regulation 178/2002, Regulation 1331/2008 and Regulation 1333/2008. Regulation 178/2002 outlines the general principles of food law in the European Union and establishes the European Food Safety Authority (EFSA). Regulation 1331/2008 establishes a common authorisation procedure for food additives, food enzymes and food flavourings and Regulation 1333/2008 deals with the conditions of use of food additives, the labelling of additives sold as such and provides the Community list of additives. This chapter provides the requirements of these Regulations and includes the implications of changes in consumer perception of additives in the use of colours and colouring foodstuffs.
Ag2S quantum dot theragnostics
Authors: Purushothaman Baskaran , Song Joon Myong
Published: October 2020

Silver sulfide quantum dots (Ag2S QDs) as a theragnostic agent have received much attention because they provide excellent optical and chemical properties to facilitate diagnosis and therapy simultaneously.
Engineering multicomponent metal-oxide units for efficient methane combustion over palladium-based catalysts
Authors: Chen Yelin , Lin Jia , Chen Xiaohua , Fan Siqin , Zheng Ying
Published: October 2020

A composition modulation strategy was exploited to rationally design high-performance Mg-promoted Pd/CexZr1−xO2–Al2O3 catalysts for methane combustion.
A thermoregulated phase transfer chiral Pt nanocatalyst for enantioselective hydrogenation of α-ketoesters
Authors: Chen Pu , Wang Yanhua
Published: October 2020

An efficient and recyclable thermoregulated phase transfer chiral Pt nanocatalyst was developed and applied to the enantioselective hydrogenation of α-ketoesters.
An overview of the uses of per- and polyfluoroalkyl substances (PFAS)
Authors: Glüge Juliane , Scheringer Martin , Cousins Ian T. , DeWitt Jamie C. , Goldenman Gretta , Herzke Dorte , Lohmann Rainer , Ng Carla A. , Trier Xenia , Wang Zhanyun
Published: October 2020

Systematic description of more than 200 uses of PFAS and the individual substances associated with each of them (over 1400 PFAS in total).
Isoorientin exerts a protective effect against 6-OHDA-induced neurotoxicity by activating the AMPK/AKT/Nrf2 signalling pathway
Authors: Ma Li , Zhang Boxi , Liu Jiahe , Qiao Chunyu , Liu Yuanyuan , Li Shize , Lv Hongming
Published: October 2020

Parkinson's disease (PD) is a progressive neurodegenerative disorder that is closely associated with oxidative stress.
Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model
Authors: Xu Yetong , Curtasu Mihai Victor , Bendiks Zachary , Marco Maria L. , P. Nørskov Natalja , Knudsen Knud Erik Bach , Hedemann Mette Skou , Lærke Helle Nygaard
Published: October 2020

An AX-enriched high DF diet improved the intestinal environment and attenuated protein fermentation, while protein did not show prebiotic effects.
Nanonewton forces between Staphylococcus aureus surface protein IsdB and vitronectin
Authors: Mathelié-Guinlet Marion , Viela Felipe , Pietrocola Giampiero , Speziale Pietro , Dufrêne Yves F.
Published: October 2020

The stress-dependent binding of IsdB to vitronectin (Vn) might promote S. aureus adhesion to human cells under shear stress.
Stimuli-responsive microgels for controlled deposition of gold nanoparticles on surfaces
Authors: Wei Menglian , Xu Wenwen , Gao Feng , Li Xue , Carvalho Wildemar S. P. , Zhang Xueji , Serpe Michael J.
Published: October 2020

A variety of gold nanoparticle (AuNP) core/poly(N-isopropylacrylamide) (pNIPAm) shell microgels (Au@pNIPAm) were generated and used to control AuNP deposition on surfaces.
Protein corona meets freeze-drying: overcoming the challenges of colloidal stability, toxicity, and opsonin adsorption
Authors: Picco Agustin S. , Mondo Gabriela Borba , Ferreira Larissa Fernanda , de Souza Edmarcia Elisa , Peroni Luís Antonio , Cardoso Mateus Borba
Published: October 2020

Freeze-drying of nanoparticle suspensions in presence of pre-formed protein corona is capable of generating stable nanoformulations with improved biocompatibility, longer storage times and easier transportation.
Nanoparticles as suitable messengers for molecular communication
Authors: Fichera Luca , Li-Destri Giovanni , Tuccitto Nunzio
Published: October 2020

Here we propose the use of nanoparticles for a new paradigm of communication occurring through interchange of information particles.
Increased spatial randomness and disorder of nucleates in dark-phase electrodeposition lead to increased spatial order and pattern fidelity in phototropically grown Se–Te electrodeposits
Authors: Simonoff Ethan , Van Muñoz Lorenzo X. , Lewis Nathan S.
Published: October 2020

Using Se–Te as a model system, we demonstrate a fundamental light–matter interaction and show how increased spatial disorder of dark-phase nucleates intrinsically produces increased long-range order of photoelectrochemically deposited nanostructures.
Beyond osmium: progress in 1,2-amino oxygenation of alkenes, 1,3-dienes, alkynes, and allenes
Authors: Hemric Brett N.
Published: October 2020

Olefin 1,2-difunctionalization is a popular synthetic strategy for synthesis of vicinal amino alcohols and their derivatives. This comprehensive review covers methods developed beyond the pioneering osmium-catalyzed Sharpless amino hydroxylation.
Free-standing and flexible 0D CeO2 nanodot/1D La(OH)3 nanofiber heterojunction net as a novel efficient and easily recyclable photocatalyst
Authors: Wang Kun , Liang Yujun , Yang Jian , Yang Gui , Zeng Zikang , Xu Rui , Xie Xianjun
Published: October 2020

Novel 0D CeO2 nanodot/1D La(OH)3 nanofiber heterojunction net with in-built Ce4+/Ce3+ redox centers was fabricated, which exhibits excellent photocatalytic performance and remarkable recoverability.
Recent progress of aggregation-induced emission luminogens (AIEgens) for bacterial detection and theranostics
Authors: Feng Xuping , Tong Bin , Shi Jianbing , Zhao Changsheng , Cai Zhengxu , Dong Yuping
Published: October 2020

The emergence and global spread of bacterial infections in clinical practice has become a public health problem. We provide a detailed and comprehensive summary of the application of AIEgens for bacterial detection and treatments.
Highly fluorescent aryl-cyclopentadienyl ligands and their tetra-nuclear mixed metallic potassium–dysprosium clusters
Authors: Arumugam Selvakumar , Reddy Pulikanti Guruprasad , Francis Maria , Kulkarni Aditya , Roy Sudipta , Mondal Kartik Chandra
Published: October 2020

Alkyl substituted triaryl-cyclopentadienyl ligands with aggregation-induced emission enhancement (AIEE) properties and their applications in the syntheses of novel chloride bridged tetra-nuclear mixed potassium–dysprosium metallocenes.
Two Ln-based metal–organic frameworks based on the 5-(1H-1,2,4-triazol-1-yl)-1,3-benzenedicarboxylic acid ligand: syntheses, structures, and photocatalytic properties
Authors: Yuan Fei , Yuan Chunmei , Cao Baoyue , Di Youying , Wang Shumin , Liu Mingbao , Kumar Abhinav , Shi Chuncheng , Muddassir Mohd.
Published: October 2020

Two new 3D Ln-based complexes showing (6,8)-connected topology were synthesized and the photocatalytic activity of the Sm(iii)-based MOF towards the degradation of methyl violet (MV) in water explored.
Low-temperature operating ZnO-based NO2 sensors: a review
Authors: Xuan Jingyue , Zhao Guodong , Sun Meiling , Jia Fuchao , Wang Xiaomei , Zhou Tong , Yin Guangchao , Liu Bo
Published: October 2020

A comprehensive review on designs and mechanisms of ZnO-based NO2 gas sensors operated at low temperature.
Aggregation-induced phosphorescence sensitization in two heptanuclear and decanuclear gold–silver sandwich clusters
Authors: Lu Zhou , Yang Yu-Jie , Ni Wen-Xiu , Li Mian , Zhao Yifang , Huang Yong-Liang , Luo Dong , Wang Xiaoping , Omary Mohammad A. , Li Dan
Published: October 2020

The strategy of aggregation-induced emission enhancement (AIEE) has been proven to be efficient in wide areas and has recently been adopted in the field of metal nanoclusters.
Direct-current energy generators from polypyrrole-coated fabric/metal Schottky diodes with considerably improved output
Authors: Wang Wenyu , Han Xing , Niu Jiarong , Jin Xin , Wang Hongxia , Shao Hao , Lin Tong
Published: October 2020

A Schottky diode made of polypyrrole-coated fabric and aluminum metal under compressive impacts can generate 4.6 times higher voltage output and 3.4 times higher power than those made of dense polypyrrole plates and aluminum metal.
Comment on “Physicochemical stimuli as tuning parameters to modulate the structure and stability of nanostructured lipid carriers and release kinetics of encapsulated antileprosy drugs” by R. Kanwar, M. Gradzielski, S. Prevost, G. Kaur, M. S. Appavou and S. K. Mehta, J. Mater. Chem. B, 2019, 7, 6539
Authors: Kang Jonghoon , Kang Albert M.
Published: October 2020

Entropy–enthalpy compensation describes a push–pull phenomenon in the binding of drug loaded nanostructured lipid carriers to bovine serum albumin.
The current state of the art of plasmonic nanofibrous mats as SERS substrates: design, fabrication and sensor applications
Authors: Yadav Sangeeta , Satija Jitendra
Published: October 2020

Strategies for the fabrication of plasmonic nanofibrous mats and their applications as SERS-active substrates that allow molecular fingerprint-based ultrasensitive detection through an enhanced electromagnetic field generated by metal nanoparticles.
Human hair derived uPA loaded capsules with dual near-infrared (I and II biowindows) laser responsive capabilities for multi-effective thrombolysis therapy
Authors: Fang Cuifu , Zhong Zhiwei , Zhang Teng , Jia Shuang , Ding Xingwei , Zhou Weimin , Wang Xiaolei
Published: October 2020

Problems such as massive hemorrhage caused by uncontrolled drug dosage are the main significant obstacles in clinical thrombolytic therapy, which are prominently due to the lack of targeting and controlled release of efficient thrombolytic drug systems.
Stacked residual blocks based encoder–decoder framework for human motion prediction
Authors: Liu Xiaoli , Yin Jianqin
Published: October 2020

Human motion prediction is an important and challenging task in computer vision with various applications. Recurrent neural networks (RNNs) and convolutional neural networks (CNNs) have been proposed to address this challenging task. However, RNNs exhibit their limitations on long‐term temporal modelling and spatial modelling of motion signals. CNNs show their inflexible spatial and temporal modelling capability that mainly depends on a large convolutional kernel and the stride of convolutional operation. Moreover, those methods predict multiple future poses recursively, which easily suffer from noise accumulation. The authors present a new encoder–decoder framework based on the residual convolutional block with a small filter to predict future human poses, which can flexibly capture the hierarchical spatial and temporal representation of the human motion signals from the motion capture sensor. Specifically, the encoder is stacked by multiple residual convolutional blocks to hierarchically encode the spatio‐temporal features of previous poses. The decoder is built with two fully connected layers to automatically reconstruct the spatial and temporal information of future poses in a non‐recursive manner, which can avoid noise accumulation that differs from prior works. Experimental results show that the proposed method outperforms baselines on the Human3.6M dataset, which shows the effectiveness of the proposed method. The code is available at https://github.com/lily2lab/residual_prediction_network.
Analyse physico-mécanique d’un composite paille de riz/ciment
Authors: Serifou Mamery Adama , Jolissaint Obre Sery Paul , Kouassi Bleh Raoul , Edjikémé Emeruwa
Published: October 2020

En raison des nombreux avantages qu’offrent les fibres végétales, elles sont de plus en plus utilisées pour la production de matériaux composites notamment dans le domaine de la construction. Le riz est la céréale la plus consommée dans le monde pour l’alimentation humaine. Sa production engendre des quantités importantes de paille qui sont peu ou pas valorisées d’où le besoin d’en faire un renfort dans les matériaux cimentaires. À travers cette étude, des composites de pailles de riz (PDR) / ciment ont été élaborés avec différentes teneurs en paille. Des essais mécaniques et physiques ont été réalisés à différents âges pour en évaluer les performances. Il en ressort que l’incorporation de 1 à 5 % de PDR induit une réduction des propriétés physiques et mécaniques des composites et provoquent un retard de prise du ciment. Cela a pour cause la grande absorption d’eau des pailles et leur faible adhésion avec la matrice cimentaire. Par ailleurs on assiste à l’allègement des composites grâce à l’introduction d’air occlus. Cette étude constitue la première étape de toute une série. Elle a permis de comprendre le comportement des PDR vis-à-vis du ciment. Les perspectives visent l’optimisation des propriétés mécaniques et physiques du matériau à travers l’incorporation de granulats.
Human resources policy in relation to cybernetic security in Slovak medium and small companies
Authors: Beláňová Benita
Published: October 2020

Due to a mass penetration of information technologies into all business processes, researches in area of IS/IT field within medium and small companies has gained on significance. With the aim to acknowledge the status of information safety in the environment of Slovak medium and small companies a research has been carried out, which focused on identifying: threats on information systems; methods for treating information safety; and major obstacles in implementation of secure and safe policy. Within this research a special part was dedicated to companies’ human resources policy in relation to cybernetic security. The research separately focused on staff provisioning in area of IS/IT. It surveyed who and on what position is responsible for this field, what requirements should be fulfilled by such people, which knowledge is the most valued and which they miss. The second area we focused on, was the organization of the security policy in relation to all employees, but mainly to users of information systems. The research was performed in the years 2008, 2012, 2015 and 2017 and the results about human resources are presented in this article. The respondents were company owners, managers and specialists form IS/IT field.
Sustainable Development and its Impact on the Quality of Human Capital
Authors: Bukovová Sylvia , Foltínová Ľubica
Published: October 2020

The quality of human capital depends on the conditions in which people live, work and learn. Therefore, the issue of sustainable development as a prerequisite for ensuring the existence and development of human capital is at the center of attention of the governments of each country. The article describes sustainable development, presents its goals and strategies to be achieved at European and national level. The aim of the research is to assess the current state and development of selected indicators of sustainable development related to the quality of human capital in Slovakia and other V4 countries in the period from 2014 to 2018.
Cooperation of Business Intelligence and Big Data in one Ecosystem
Authors: Černý Matej
Published: October 2020

This paper is focused on the issue, how the business can analyze all data types (structured and unstructured) in one cooperative environment. With structured data handle Business Intelligence and with unstructured data on the other side Big Data. As a solution to this issue, we have suggested our Business Intelligence and Big Data ecosystem. This model - the ecosystem is based on already proven data processing processes running in Business Intelligence and in Big Data areas. Both processes are integrated into one unit. We have also described their common functioning.
Development of Tax Stimulation and Business Environment in Slovakia
Authors: Ďurinová Ivona , Tačarová Natália
Published: October 2020

Tax incentives are specific and targeted tax instruments in the economic and social policy of the state. With appropriate tools, the state can support the creation of new jobs and also the sustainability of human resources in existing companies. The article deals with the issue of stimulation of companies in Slovakia and the suitability of the applied incentives in connection with the business environment. The aim of the paper is to approach and analyze the impact of tax incentives applied in the Slovak Republic and evaluate them based on their impact on business entities, respectively business environment. The analysis of the overall development of provided tax incentives in the Slovak Republic is focused on the period from 2002 to 2018. The paper uses the method of analysis and synthesis together with the methods of tabular and graphical representation. Mathematical and statistical methods are used in individual calculations. On the one hand, it can be stated that tax incentives are important, even necessary, for countries, given the inflow of foreign capital, but on the other hand, domestic businesses are disadvantaged by which foreign investors disrupt the domestic business environment.
Model H application to determine synergic effects on merger and acquisition
Authors: Harumová Anna
Published: October 2020

Synergistic effects arise as effects of joint action based on mergers or acquisitions. Bundling is a common part of the growth strategy. A merger may acquire forms of merger or merger. Acquisition represents the acquisition of the ownership and management value of one company over another. Theory distinguishes in this case from property acquisitions in which the acquisition of the company's assets and capital acquisitions occurs, in which the acquisition of a decisive share in the voting rights of the company is obtained. Reasons for merger and acquisition are to gain more market share, restructure entities, improve balance of payments, and so on. The success of the merger and the acquisition confirms the emergence of a synergy effect. In determining the value of synergies in this article was used newly created Model H, which is based on a valuation of the business enterprise.
On the eve of tomorrow
Authors: Korcsmáros Enikő , Csinger Bence
Published: October 2020

The main purpose of our research is to examine the role of social media in the marketing strategy of small and medium-sized enterprises. The reason for our choice of topic is that social media is extremely important nowadays, which also influences the marketing strategy of the sector under investigation. For this reason, we examined the relevance of this topic in the Central and Western Transdanubian region. In the first part of our research, we collacted information on social media concepts and their distribution internationally and domestically. As a next step, we focused on presenting the most common social media and the opportunities they offer. Then we provide an insight of the situation of the regions under study, and the author’s hypotheses are formulated, followed by an examination of their relevance.
Characteristics of expert´s report as evidence
Authors: Kubica Milan , Švejdová Nikola
Published: October 2020

In recent years, there has been an increasing need for private expert´s report for judicial evidence. In practise, it appears that a well-developed expert´s report is an important bases for the court´s decision-making. It is no secret that the quality of the expert´s reports presented varies. Therefore, when expert drawing up his expert´s report, either directly for the court or for the parties, the expert should avoid mistakes and pay attention to the quality of the expert´s report, which should have certain characteristics that we address in this paper.
Methods used in personnel audit in companies operating in the Slovak Republic in the stage of Industrial Revolution 4.0
Authors: Matkovčíková Natália
Published: October 2020

The present paper contains the results of a research carried out in the framework of the VEGA project 1/309/18. Its aim was to identify and classify methods and approaches used in personnel audit in companies operating in the Slovak Republic in the stage of Industrial Revolution 4.0. to obtain objective information on compliance with legal standards and established standards for personnel work in the company in two areas: human resources and human resources management. The paper contains an analysis and description of the changes that have occurred in the companies with the onset of the Industrial Revolution 4.0 and the progress that these changes brought about in the repertoire of methods and approaches used in personnel audit. Key words: personnel audit, stage of industrial revolution 4.0, methods of personnel audit.
Distributional innovations of copyrighted works under conditions of sustainability
Authors: Matušovič Martin
Published: October 2020

Our research in the field of intellectual property has focused on selected innovative forms of distribution and protection. In examining classical innovations, we found different perspectives on their nature. Recently, we have witnessed social challenges and phenomena, which theory, practice and respondents understand as "social" innovations. The results of research into robotics, artificial intelligence, the Internet of Things and communication technologies need to be radically improved. The current epidemiological situation also significantly affects intellectual property in relation to sustainability. In terms of intellectual property records in society, our research is based primarily on generally binding internal standards, in particular our own and, in part, external resources in the field of intellectual property and innovation. Our research on boars also focused on the distribution of intangible products in pandemic conditions. We used the methodology of quantitative survey and quantitative statistical methods. Data were collected as metadata and also by direct collection. Subsequently analysed by statistical program by correlation and regression analysis. The results show that companies are aware of the importance of all kinds of innovation, intellectual property products and a significant change in distribution channels.
How a pricing decisions are decided and executed
Authors: Remeňová Katarína , Kintler Jakub
Published: October 2020

Marketing and management studies are rich in the theoretical backround related to price strategy and pricing. However, consistent with the lack of interest in complexity of decision making process in price management. The purpose of the research study is to identify all stages in decision – making process in price management in Slovak enterprises and to measure their mutual association and their dependence on elements of organizational parameters. Based on the results of descriptive statistics on decision making process in price management, we can say that companies go through an average of four steps in the decision-making process (M = 4.72), with higher variability (SD=3.02), compared to the mean.