bluuebunny/crossref_metadata_2025 huggingface dataset:
Morpho-Molecular Characterization and Screening of Rice (Oryza Sativa L.) Genotypes for Salinity Tolerance at Seedling Stage
Authors: Muti SAA , Hoque MI , Islam MM , Siddique MA , Islam MS
Published: March 2021

Salinity is one of the major abiotic stresses which severely affect the production of crops across the world. Twenty rice genotypes of diverse origins were assessed to examine salt tolerance potentiality at seedling stage as well as to elucidate the genetic variation using SSR markers. Consequently, IRRI standard protocol was applied to screen out those varieties for tolerance at the glasshouse laboratory of Bangladesh Institute of Nuclear Agriculture, maintaining salt stress level at 6dS m-1, 8dS m-1, 10dS m-1 and 12 dS m-1. Data on morphological parameter were recorded as shoot length (cm), root length (cm) and total dry matter (TDM). For molecular characterization thirteen SSR markers were exploited to determine the genetic diversity and in this context, 53alleles were revealed while the mean number of alleles per locus was 4.The Polymorphism Information Content (PIC) value ranged from 0.37- 0.78 having an average of 0.58. The Un-weighted Pair Group Method with Arithmetic Mean (UPGMA) displayed three major clusters including some sub-clusters, where in Cluster 1, Binadhan- 8, Binadhan-10, Pokkali and FL478 were very close in their vicinity. Information obtained from this experiment, thus can be taken into account for stress breeding program and prospective genetic materials could be utilized for exploring the genetic architecture in favor of salt tolerance as well as for developing better salt tolerant rice varieties in near future.

SAARC J. Agri., 18(2): 1-15 (2020)
Germination and Seed Vigour of Indigenous Bean (Phaseolus Vulgaris) Genotype in Nepal
Authors: Uprety Anupam , Dahal Bhishma Raj , Shrestha Bishal
Published: March 2021

Bean is an important vegetable crop of Nepal but farmers of Jumla district of Nepal are experiencing low productivity, probably due to poor germination. Keeping these points in view, a study was conducted to determine the germination and seed vigour of various indigenous beans genotype of Jumla district during February to March, 2018. The promising lines of Jumli beans namely KBL-3, KBL-2, PB0002, PB0001, KBL-1 and farm saved KBL-3 were used under study. The experiment was laid in completely randomized design (CRD) with four replications and six treatments namely T1 = KBL-3, T2 = KBL-2, T3 = PB0002, T4 = PB0001, T5 = KBL-1 and T6 = Farm-saved KBL-3. Significant differences were observed in germination percentage, speed of germination, seed vigourindex and true leaf emergence among different lines of Jumli beans .The highest germination percentage (96%), seed vigour index (1367) and speed of germination (1.763) was found in the variety of KBL-3 whereas the lowest germination percentage (69%), seed vigour index (947) and speed of germination (0.968) were found in the variety of KBL-1. Days to true leaf emergence was found shortest in the KBL-3 (23.50) and longest in the farm saved KBL-3 (30.25). KBL-3 pureline of Jumli bean was found to be highly responsive to seed germination and vigour characteristics. Thus, it would be better to suggest the indigenous bean growing farmers of Jumla district to cultivate KBL-3 for better germination and seed vigour.

SAARC J. Agri., 18(2): 67-75 (2020)
Polymorphism of Sakini Chicken Population From Different Locations/Altitudes of Nepal Using Randomly Amplified Polymorphic Dna Markers
Authors: Gorkhali NA , Dhakal R , Sapkota S , Koirala P , Pokhrel BR , Kolachhapati MR , Bhattarai N
Published: March 2021

A study was conducted to evaluate genetic polymorphism in three Sakini chicken populations using random amplification of polymorphic DNA (RAPD) with seven highly polymorphic primers. All populations showed polymorphism with these primers that generates 59 different bands with an average of 8.4 bands per primer with 78.6% polymorphism nature. Primer OPA-16 produced the highest number of polymorphism bands 47 % and the lowest number of bands was produced by the OPA-05 primer 24 %. Differences for genetic distance (D) among populations were significant (P<0.05). A consensus dendogram was therefore developed to show the phylogenetic relationship among the populations. The cluster pattern is well supported by the principle component analysis that also separates all three populations of Sakini chicken into six major groups. The results provide evidence of the applicability of RAPD to determining genetic relatedness within and among different poultry populations and in developing reproducible markers useful in evaluating individual variation in poultry.

SAARC J. Agri., 18(2): 115-124 (2020)
Impact of Hydrometeorological Hazards on Agricultural Production at Chalan Beel, Bangladesh
Authors: Ahmed A , Hossain MS , Majumder AK
Published: March 2021

Agriculture of Bangladesh is closely dependent on weather, rainfall pattern and land formation. However, frequent hydro-meteorological hazards cause to adversely affect the normal agricultural process and production. This study aimed to assess the impact of Hydro meteorological hazards on the crop cultivation practices, possible mitigation and adaptation measures for the farmers at Chalan Beel area under Pabna district, Bangladesh. There are 90 people having land and engaged with crop cultivation directly at Chalan Beel were been selected by applying purposive sampling method and interviewed by a pre-designed semi-structured questionnaire through face to face interview. The results revealed that Rice (93.75 %) and Garlic (47.5 %) are the major cultivating crops at Chalan Beel and 96 % of the farmers were been experienced with hydrometeorological devastation. The farmers perceived Nor’wester (77.9 %) as the most frequent hazard causing crop damage following by Heavy Rainfall (71.4 %), Hailstorm (68.8 %), Heavy Wind (45.5 %), Flashflood (42.9 %), Heavy Fog and Floods (19.5 % and 11.7 %) respectively. It is also identified that Nor’wester (58.4 %) and Heavy Rainfall (58.4 %) had maximum damage whereas Hailstorm (55.8 %), Heavy Wind and Flashflood also considerable adverse effects on overall crop production at this area. Only 24 % of farmers were found to change their cultivation pattern as the adaptation measure. This study portrayed that hydro-meteorological hazards are adversely affecting the crop production at Chalan Beel area and resulting economic loss. Therefore, government should ensure the participation of farmers along with adopting their deliberative in developing a strategic plan for providing technical support for diversification and training to adapt in this hazard prone area.

SAARC J. Agri., 18(2): 181-193 (2020)
Hubungan antara Pemeriksaan Antibodi Dengue IgG dengan Uji Fungsi Hati (SGOT dan SGPT) pada Pasien Demam Berdarah Dengue (DBD) di RSUD dr. M. Yunus bengkulu Bulan Desember 2015 - Januari 2016
Authors: Mulyadi Mulyadi , Novelia Marlia , Nugraheni Enny
Published: March 2021

atar Belakang: Demam Berdarah Dengue merupakan suatu infeksi yang disebabkan oleh virus dengue dengan gejala klinis terjadinya demam mendadak 2-7 hari, tanda-tanda perdarahan, hepatomegali, syok apabila tidak ditegakkan secara cepat akan menyebabkan komplikasi dan kematian. Penelitian ini bertujuan untuk mengetahui hubungan pemeriksaan antibodi dengue IgG dengan uji fungsi hati SGOT dan SGPT pada pasien DBD.Metode: Penelitian ini menggunakan studi analitik dengan pendekatan cross-sectional. Penelitian dilaksanakan pada bulan Desember 2015 sampai Januari 2016 di Laboratorium RSUD dr. M. YunuS. Pengambilan sampel dilakukan dengan menggunakan teknik consecutive sampling. Penilaian Pemeriksaan antibodi dengue IgG menggunakan dengue rapid test SD Dengue IgG/IgM®. Penilaian uji fungsi hati (SGOT dan SGPT) menggunakan uji kimia Autoanalyzer (architect plus c4000®). Variabel bebas adalah antibodi dengue IgG, sedangkan variabel terikat adalah uji fungsi hati SGOT dan SGPT. Analisis data dilakukan dengan univariat dan bivariat dengan menggunakan uji statistik Koefisien Kontingensi.Hasil Penelitian: Jumlah subjek penelitian sebanyak 32 orang, terdiri dari perempuan sebanyak 20 orang (62.5%) lebih banyak daripada laki-laki sebanyak 12 orang (37.5%) dengan usia paling muda 3 tahun dan usia paling tua 65 tahun. Hasil analisis bivariat menunjukkan bahwa nilai SGOT p=0.149 dan SGPT p=0.108 (p > 0.05) tidak terdapat hubungan yang bermakna antara dua variabel yang diuji dan nilai korelasi Koefisien Kontingensi SGOT r 0.247 dan SGPT r 0.273 menunjukkan bahwa arah korelasi positif dengan kekuatan korelasi lemah.Simpulan: Terdapat hubungan positif lemah tidak bermakna antara pemeriksaan antibodi dengue IgG dengan uji fungsi hati (SGOT dan SGPT) pada pasien demam berdarah dengue (DBD) di RSUD M. Yunus Bengkulu bulan Desember 2015 sampai Januari 2016.Kata kunci: Antibodi dengue IgG, SGOT, SGPT, DBD
PERAN TUMOR ASOCIATED MACROPHAGE (TAM) PADA KANKER PAYUDARA
Authors: Taurina Hilda
Published: March 2021

ABSTRAKKanker payudara adalah salah satu kanker yang banyak ditemukan di dunia. Makrofag yang dijumpai pada kanker dikenal dengan istilah Tumor Asociated Macrophage (TAM) yang meliputi fenotip makrofag M1 dan M2. Kedua fenotip ini mempunyai fungsi yang berlawanan.Kata Kunci : Kanker payudara, Makrofag M1, Makrofag M2
EKSPRESI p53 DAN E-CADHERIN SEBAGAI PREDIKTOR PROGNOSIS PADA KARSINOMA SEL SKUAMOSA RONGGA MULUT DI RSUP DR. KARIADI SEMARANG
Authors: Pangaribuan Clara
Published: March 2021

p53 AND E-CADHERIN EXPRESSION AS  PREDICTORS OF PROGNOSTIC IN ORAL SQUAMOUS CELL CARCINOMA AT KARIADI HOSPITAL SEMARANG
KORELASI ANTARA EKSPRESI Her-2 DAN Ki-67 DENGAN GLEASON GRADE GROUP PADA ADENOKARSINOMA ASINAR PROSTAT
Authors: Larasati Putri ajeng ayu
Published: March 2021

Latar Belakang : Menurut Database GLOBOCAN 2012, kanker prostat merupakan penyebab utama kelima kematian pada pria.(1) Pemahaman tentang peran onkogen dan tumor suppressor genes mendominasi penelitian tentang biologi kanker saat ini dan berpotensi menghasilkan target terapi kanker terbaru. Salah satu perannya pada adenokarsinoma prostat yang masih belum jelas ialah Her-2 dan Ki-67. Perbedaan ekspresi profil molekular Her-2 dan Ki-67 yang diklasifikasikan berdasarkan Gleason grading system terbaru yaitu Grade group diharapkan membantu penentuan prognosis dan manajemen terapi penyakit pada kanker prostat.Tujuan : Tujuan dari penelitian ini adalah untuk mengetahui korelasi antara ekspresi Her-2 dan Ki-67 dengan Gleason Grade group adenokarsinoma asinar prostat.Metode: Merupakan penelitian observatif analitik dengan desain cross-sectional menggunakan 31 blok parafin yang terfiksasi formalin dari laboratorium Patologi Anatomik RSUP dr. Kariadi, dengan diagnosis adenokarsinoma asinar prostat kemudian diklasifikasikan menurut kelompok prognostik WHO / ISUP Gleason (Gleason Grade group 1 - 5). Pemeriksaan imunohistokimia dilakukan dengan menggunakan antibodi Her-2 dan Ki-67. Analisa hasil menggunakan uji Spearmans dan uji Kruskal WallisHasil : 31 kasus yang termasuk dalam kriteria inklusi pada penelitian ini, hampir separuhnya (41.94%) termasuk ke dalam Gleason grade group 5. Ekspresi Her-2 positif (+1, +2, +3) hampir didapatkan pada seluruh sampel (90,22%), dengan Her-2 +3 sebagian besar didapatkan pada grade group 5 (71,43%). Ekspresi Ki-67 positif (+1, +2, +3) didapatkan pada seluruh sampel, dengan Ki-67 +3 (>5%) pada Gleason grade group 5 didapatkan sejumlah 5 sampel (38,46%). Pada analisa data dengan uji Spearmans disimpulkan bahwa terdapat korelasi positif antara ekspresi Her-2 dan Ki-67 dengan Gleason Grade group. Pada uji Kruskal Wallis didapatkan perbedaan bermakna antara ekspresi Ki-67 dengan Gleason Grade group.Kesimpulan: Analisa ekspresi Her-2 dan Ki-67 melalui pemeriksaan imunohistokimia dengan klasifikasi terbaru Gleason Grade group dapat menjadi salah satu parameter prognosis dan manajemen terapi pada adenokarsinoma asinar prostat.Kata kunci : Adenokarsinoma asinar prostat, Her-2, Ki-67, Gleason Grade group
THE EFFECTIVENESS OF CORIANDER (Coriandrum sativum L.) EXTRACTS ON REDUCING THE BLOOD SUGAR LEVELS OF ALLOXAN INDUCED WISTAR MALE RATS (Rattus norvegicus L.)
Authors: Dersing Kalvin
Published: March 2021

AbstractBackground : Diabetes mellitus is the most common metabolic syndrome in the whole world with an incidence of 1-8%. This disease arises when insufficient insulin is produced or insulin cannot function properly.The ability of coriander juice water extract is expected to reduce blood glucose levels because the action is similar to insulin and can stimulate insulin release. Purpose : Knowing effectiveness coriander extract (Coriandrum sativum L.)to decline blood sugar levels in male white rats (Rattus norvegicus L.) wistar strain induced by alloxan. Method : This is a pure experimental study (true-experiment) using a pre and post test design with control group design using subjects namely white rats (Rattus norvegicus L.) male Wistar strain aged 10-12 weeks with a weight of 150-200 grams. By looking at changes in blood sugar levels during administration of alloxan and administration of coriander extract. Results : There were significant changes in some groups induced alloxan (p <0.05), but the effect of coriander extract was not very significant (p> 0.05). Conclusion : Coriander extract can control blood sugar levels to be stable or normal, but it takes longer and more research.  Keywords : coriander extract, blood sugar levels, alloxan
KETOKONAZOL 2% LEBIH EFEKTIF DIBANDING EKSTRAK BUNGA SEPATU (Hibiscus rosa sinensis L.) SEBAGAI HERBAL POTENSIAL ANTI MIKOSIS
Authors: Alya Qonitah Anggara
Published: March 2021

Latar Belakang Malassezia furfur merupakan flora normal kulit kepala yang dapat menjadi patogen penyebab ketombe dan dermatitis seboroik. Penggunaan shampo antiketombe yang mengandung berbagai senyawa kimia dalam jangka panjang dapat menimbulkan efek samping yang membahayakan. Beberapa isolat Malassezia furfur bersifat resisten terhadap golongan azol. Bunga sepatu (Hibiscus rosa sinensis L.) mengandung senyawa metabolit antijamur. Tujuan Membandingkan efektivitas ketokonazol 2% dan ekstrak etanol bunga sepatu sebagai antijamur terhadap pertumbuhan Malassezia furfur. Metode Penelitian eksperimental laboratorium dengan post test only control group design dilakukan secara triplo menggunakan 6 kelompok perlakuan dengan konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, dan 3,125%. Kontrol positif (+) berisi media Sabouraud Dextrose Broth (SDB) olive oil & suspensi jamur sedangkan kontrol negatif (-) berisi media SDB olive oil, suspensi jamur, & ketokonazol 2%. Penentuan Kadar Hambat Minimal (KHM) memperhatikan kekeruhan media SDB olive oil, dilanjutkan penggoresan pada Sabouraud Dextrose Agar (SDA) olive oil dengan mengamati pertumbuhan koloni untuk menentukan Kadar Bunuh Minimal (KBM). Sampel inkubasi 2-5 hari dengan suhu 37°C. Hasil Pengamatan semua konsentrasi ekstrak etanol bunga sepatu pada hari 1-5 ditemukan pertumbuhan jamur sedangkan pengamatan ketokonazol 2% pada hari 1-2 tidak ditemukan pertumbuhan jamur. Kesimpulan Ketokonazol 2% lebih efektif dibandingkan dengan ekstrak etanol bunga sepatu dalam menghambat pertumbuhan Malassezia furfur.Kata kunci : Efektivitas antijamur, Ketokonazol 2%, Ekstrak bunga sepatu, Malassezia furfur.
The future of Funds for Innovative Medicines: results from a Delphi Study
Authors: Jommi Claudio , Armeni Patrizio , Bertolani Arianna , Costa Francesco , Otto Monica
Published: March 2021

Introduction: Dedicated Funds for Innovative Medicines were introduced in 2017 for cancer and non-cancer drugs in Italy. After three years, their impact on patient access to the relevant treatments and critical issues about their management has been poorly investigated.

Aims and scope. This paper aims at bridging the literature gap and providing possible reforms scenarios.

Methods: Our analysis relied on a qualitative approach. The personal opinions of twelve Italian experts coming from the Ministry of Economy and Finance, the Scientific Committee of the Italian Medicines Agency, the Regions and Patient Associations, the Oncologists’ Scientific Societies and Hospital Pharmacists were elicited through a Delphi approach. A consensus on final recommendations was reached in two rounds.

Results: Experts were in favour of maintaining dedicated Funds for Innovative Medicines and had a distinct preference for a single Fund. Most of them suggested to extend access to Funds to more than three years, if, for the relevant indication, there are no alternatives to the innovative drug and provided that this does not represent a barrier to new entries. Responders advocated for Funds being covered by on top resources and the production of more evidence on their impact. They finally claimed a speeder flow of information to the regions on expenditure for innovative treatments and an enhancement of controls on prescribing behaviour, to avoid prescriptions be dependent on Funds capacity.

Conclusions: The consensus document provides for eight recommendations that could be taken into account for possible reforms and future research on this topic.
Gestante con COVID - 19 y Diabetes Mellitus con evolución desfavorable
Authors: Gamarra-Manrique Renzo R. , Riveros-Alvaro Jael D.
Published: March 2021

Se presenta un caso de multigesta con edad gestacional avanzada, de 35 años, quien cursó con gestación de 25 semanas e infección por SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2). Tenía antecedente de diabetes mellitus II con tratamiento y controles irregulares. La paciente estuvo asintomática por 5 días tras el diagnóstico, presentando distress respiratorio y compromiso de sensorio en el lapso de 24 horas. Ingresa por emergencia, presentando falla multiorgánica y falta de latidos fetales, por lo que es transferida a UCI (unidad de Cuidados Intensivos). Se le realiza cesárea tras compensación hemodinámica. El producto es un óbito fetal de 24 semanas. Continúa con tratamiento en UCI, donde fallece 19 días después. Se recomienda considerar el seguimiento más estrecho en gestantes con enfermedades concomitantes, como diabetes.
Conocimientos sobre lactancia materna en madres primerizas. Instituto Nacional Materno Perinatal, Lima 2019
Authors: Alvarez Lopez María del Mar , Angeles Salcedo Alessandra Patricia , Pantoja Sanchez Lilian R.
Published: March 2021

Objetivo: Determinar el nivel de conocimiento sobre lactancia materna en madres primerizas del Instituto Nacional Materno Perinatal en Julio 2019. Materiales y Métodos: Estudio cuantitativo, descriptivo, transversal. Se encuesto previo consentimiento informado a 276 madres primerizas puérperas del INMP usando un cuestionario validado. Resultados: El 80,4 % de las madres primerizas obtuvo un nivel de conocimiento regular mientras que el 8,3% obtuvo un nivel de conocimiento malo. El mayor porcentaje de madres con nivel de conocimientos buenos sobre lactancia materna estaba entre los 24 y 29 años (38,71%), en cambio, las madres menores o igual a 23 años presentan el mayor porcentaje de nivel de conocimiento malo (47,82%). Asimismo, las madres provenientes de provincia representaron el mayor porcentaje de nivel de conocimientos malos (73,91%) siendo más del doble de las que procedían de Lima (26,1%). Se comprobó que existe una asociación entre la procedencia y la ocupación de la madre con el nivel de conocimiento. Conclusiones: predominó un nivel de conocimiento regular sobre el conocimiento de lactancia materna. En cuanto al perfil de la madre con mal conocimiento fue aquella menor de 23 años, conviviente, procedente de provincia, que trabaja y con estudios secundarios completa; mientras que el perfil de la madre con buen conocimiento fue aquella entre 24 y 29 años, conviviente, procedente de Lima, ama de casa como ocupación y con estudio superior incompleto.
Rol del Instituto Nacional Materno Perinatal en la reducción de la mortalidad materna y neonatal
Authors: Guevara-Ríos Enrique
Published: March 2021

El 10 de octubre de 1826 el Mariscal don Andrés de Santa Cruz, quien, por decreto supremo emitido por Hipólito Unanue, creó la Casa de Maternidad, centro hospitalario que tendría como finalidad la atención de las parturientas, donde además se impartiría preparación en la práctica obstétrica para la formación de comadronas instruidas y hábiles1.

En 1943, durante el primer gobierno de don Manuel Prado, por decreto supremo, se le da la denominación oficial de Hospital de Maternidad de Lima. En 2002 logra la denominación de Instituto Especializado Materno Perinatal y desde el 2006 es categorizado como Instituto Nacional Materno Perinatal 2.

 La misión del INMP es brindar atención especializada a las mujeres, gestantes y recién nacidos de alto riesgo, desarrollando investigación, tecnologías y docencia a  nivel nacional 3. En su rol de establecimiento de tercer nivel tiene como políticas 4: el acceso oportuno a los servicios altamente especializados en las mujeres en la atención de la salud sexual y reproductiva y acceso a las gestantes y recién nacidos en el área materno perinatal, contribuyendo a la formación de redes de servicios efectivas para la atención, con énfasis en la atención de alta complejidad; el fortalecimiento de la capacidad resolutiva para la alta complejidad de los principales hospitales del país con atención materno perinatal, mejorando las competencias del talento humano; y promover la docencia e investigación materna perinatal, el desarrollo de nuevas tecnologías, innovación y modelos de atención.

 El INMP tiene como objetivo general Brindar atención altamente especializada y de calidad en salud sexual y reproductiva con énfasis en las gestantes y neonatos de alto riesgo que acudan al INMP y que sean referidas de otros establecimientos de salud de Lima y de todas las regiones del país. Sus objetivos específicos son:

Atención altamente especializada en obstetricia crítica, medicina materno-fetal, medicina reproductiva y en neonatología crítica.

Desarrollo del potencial humano de la institución, a nivel nacional e internacional.

Promoción de investigaciones que puedan contribuir a mejorar la salud de las mujeres, gestantes y neonatos en el país.
Identificação dos diagnósticos de enfermagem em recuperação pós-anestésica segundo a Teoria das necessidades humanas básicas
Authors: Borges Karen Rocha , Bernardo Thaís Honório Lins , Soares Imaculada Pereira , Sarmento Patrícia de Albuquerque , Bastos Maria Lysete de Assis , Santos Amuzza Aylla Pereira dos , Souza Evandro Everson Silva
Published: March 2021

Na sala de recuperação pós-anestésica são realizados cuidados intensivos em pacientes no pós-operatório imediato de procedimento anestésico-cirúrgico, permanecendo sob observação até o momento em que estejam conscientes, com reflexos protetores existentes e com os sinais vitais estáveis. De tal modo, o presente estudo visa identificar os diagnósticos de enfermagem mais frequentes em sala de recuperação pós-anestésica segundo a Teoria das necessidades humanas básicas. Trata-se de uma pesquisa de natureza quantitativa, do tipo descritivo com delineamento transversal, feita com um instrumento validado para a coleta dos dados, aplicado em pacientes maiores de 18 anos que passaram por atendimento cirúrgico, sendo admitidos na sala de recuperação pós-anestésica de uma unidade hospitalar de grande porte, referência em assistência de alta complexidade no Sistema Único de Saúde da região nordeste do Brasil. A amostra do estudo compôs-se de 48 pacientes, com trinta e duas mulheres (66,7%) e dezesseis homens (33,3%). Foram identificados 22 diagnósticos de Enfermagem, destes 17 corresponderam às necessidades humanas básicas psicobiológicas, 4 às necessidades humanas básicas psicossociais e 1 psicoespiritual. Diagnósticos encontrados em 100% da amostra: Risco de Infecção, Risco de Queda, Risco de Desequilíbrio da Temperatura Corporal, Risco de Aspiração, Integridade da Pele Prejudicada e Mobilidade no Leito Prejudicada. Em suma, os diagnósticos identificados são achados comuns em pacientes de pós-operatório imediato, sendo possível antecipar as ações e evitar que ocorram essas complicações e o agravamento da condição de saúde do paciente.
Composição química, atividade antimicrobiana e antioxidante do óleo essencial de folhas Myrcia palustris DC. (MYRTACEAE)
Authors: Santos Camila Vogt dos , Mallmann Ana Paula , Toledo Adrieli Gorlin , Bandeira Débora Marina , Costa Willian Ferreira da , Marins Daíse Miranda Ávila , Corrêa Juliana Moço , Pinto Fabiana Gisele da Silva
Published: March 2021

O Brasil apresenta a maior biodiversidade no mundo e neste contexto, suas espécies nativas tornam-se uma alternativa na busca por moléculas bioativas para bioprospecção de antimicrobianos e antioxidantes naturais. Pertencente à família Myrtaceae, a espécie Myrcia palustris DC, conhecida popularmente como pintagueira-do-mato, não apresenta estudos referente às suas atividades biológicas e composição química. Diante disso, o objetivo deste trabalho foi determinar a composição química do óleo essencial de M. palustris por cromatografia gasosa acoplada à espectrometria de massas (CG-EM), avaliar sua atividade antimicrobiana pela técnica de microdiluição em caldo e a atividade antioxidante pela técnica de 2,2-difenil-1-picril-hidrazila (DPPH). O rendimento total a partir da extração pela técnica de hidrodestilação do óleo essencial foi de 0,35%. A CG-EM revelou a presença de 28 compostos, sendo a maioria da classe dos sesquiterpenos. A atividade antimicrobiana foi observada para todas as bactérias Gram-positivas (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus) com exceção de Staphyloccocus epidermidis. Em relação às Gram-negativas, verificou-se atividade inibitória apenas para Shigella flexneri. Para levedura Candida albicans não foi observada atividade inibitória e nem fungicida. O óleo essencial apresentou capacidade de redução de radicais de DPPH até 82,81%, confirmando seu potencial antioxidante. Sugere-se que a ação antimicrobiana e antioxidante presentes no óleo essencial de M. palustris esteja relacionada à presença dos compostos majoritários α-Guaieno (25,89%), α-Bulneseno (13,39%) e β-Selineno (4,76%).
Keanekaragaman Koloni Mikroorganisme Rizosfer Lahan Tebu (Saccharum officinarum) Pada Penggunaan Pupuk Bio-Slurry dan Pupuk Kimia
Authors: Ismy Adifatul , Syauqi Ahmad , Zayadi Hasan
Published: March 2021

The diversity of microorganisms is very important in the balance of soil ecosystems is also an indicator of soil health and can affect the condition of plants that grow on it. Rizosphere is a part of the soil around the root of a plant. The activity of some rhizosphere microorganisms plays a role in the nutrient cycle and the process of soil formation, plant growth, affecting microorganism activity, and as biological control of root pathogens. The research objective was to determine the value of the sugarcane (Saccharum officinarum) rhizosphere microorganism index using Bio-slurry and chemical fertilizers. This study uses descriptive explorative methods. The data analyzed in the form of measurement of pH, number of microorganism colonies and diversity index. Diversity of Colonies Rhizosphere microorganisms Sugarcane land that uses Bio-slurry and those using Chemical Fertilizer are classified as low respectively 0.47 and 0.69. Colony groups found in each land are bacteria and fungi. 
Keywords: Diversity, Rizosphere, Saccharum officinarum, Bio-slurry, Chemical Fertilizers
ABSTRAK
Keanekaragaman mikroorganisme sangat penting dalam keseimbangan ekosistem tanah juga merupakan indikator kesehatan tanah dan dapat mempengaruhi kondisi tanaman yang tumbuh di atasnya. Rizosfer merupakan bagian tanah yang berada di sekitar perakaran tanaman. Aktivitas beberapa mikroorganisme rizosfer berperan dalam siklus hara dan proses pembentukan tanah, pertumbuhan tanaman, memengaruhi aktivitas mikroorganisme, serta sebagai pengendali hayati terhadap patogen akar. Penelitian ini bertujuan untuk mengetahui nilai indeks keanekaragaman mikroorganisme rizosfer tebu (Saccharum officinarum) yang menggunakan Bio-slurry dan pupuk kimia. Penelitian ini menggunakan metode diskriptif eksploratif. Data yang dianalisis berupa hasil pengukuran pH, jumlah koloni mikroorganisme dan indeks keragaman. Keanekaragaman koloni mikroorganisme rizosfer lahan tebu yang menggunakan Bio-slurry dan yang menggunakan Pupuk kimia tergolong rendah masing-masing bernilai 0,47 dan 0,69. Kelompok koloni mikroorganisme yang ditemukan pada masing-masing lahan yaitu bakteri dan jamur. 
Kata kunci: Keanekaragaman, Rizosfer, Saccharum officinarum, Bio-slurry, Pupuk Kimia
Kepadatan Populasi Ikan Gelodok Di Hutan Mangrove Desa Penunggul Kecamatan Nguling Kabupaten Pasuruan
Authors: Basri Moch. Chasan , Santoso Hari , Laili Saimul
Published: March 2021

Mangrove forests are the one of the coastal ecosystems; especially the northern coast and many are founded in the surrounding fish ecosystems. That organism is the one of the fauna which mangrove ecosystem constituent. The aim of this study was to determine the density and abundance of the population of Gelodok fish at mangrove areas in Penunggul Village, Nguling District. This study used a purposive sampling method from three stations and each station has three plots and three replications in each plot. The results showed that the density of frog fish in the mangrove forest area of ​​Nguling village at station 1 was 3 as well as at station 2 and 3 respectively 2 and 1. At station 1 there were three species, namely Baleopthalmus boddarti, Periopthalmus dipus, Periophthalmus Gracilis, station 2, there were two species; Baleopthalmus boddarti, Periophthalmus Gracilis, and station 3 there were three species namely Baleopthalmus boddarti, Periopthalmodon schlosseri, Periopthalmus dipus. The differences in the results of the study, environmental conditions and abiotic factors influence the density of fish in the mangrove area and at the station 1 the density is highe.
Keywords: Gelodok fish, mangrove forest, population
ABSTRAK
Hutan mangrove merupakan salah satu ekosistem daerah pantai terutama pantai utara dan banyak di temui ikan Gelodok di sekitar ekositem ini. Organisme tersebut merupakan salah satu fauna penyusun ekositem mangrove. Tujuan penelitian ini untuk mengetahui kepadatan dan kemelimpahan populasi ikan Gelodok kawasan mangrove desa Penunggul Kecamatan Nguling. Penelitian ini menggunakan metode purposive sampling dari tiga stasiun dan setiap stasiun terdapat tiga plot dengan ulangan tiga kali dalam setiap plot. Hasil penelitian menunjukkan bahwa kepadatan ikan gelodok di kawasan hutan mangrove desa Nguling rata-rata pada stasiun 1 adalah 3, di satsiun 2; 2 dan 3; 1. Pada stasiun 1 terdapat tiga spesies yaitu Baleopthalmus boddarti, Periopthalmus dipus, Periophthalmus Gracilis , stasiun 2 terdapat dua spesies yaitu Baleopthalmus boddarti, Periophthalmus Gracilis dan stasiun 3 terdapat tiga spesies yaitu Baleopthalmus boddarti, Periopthalmodon schlosseri, Periopthalmus dipus. Perbedaan hasil penelitian, kondisi lingkungan dan faktor abiotik mempengaruhi kepadatan ikan Gelodok di kawasan mangrove, sehingga pada stasiun 1 diperoleh kepadatan  lebih tinggi.
Kata kunci: ikan Gelodok, hutan mangrove, kepadatan populasi
Pengaruh Fraksi Ejakulasi terhadap Motilitas Spermatozoa Kambing Peranakan Etawa (Capra aegagrus)
Authors: Nahdiyah Ayu Naila , Santoso Hari , Zayadi Hasan
Published: March 2021

The ejaculation fraction on Etawa crossbred goat (PE goat) has various standard spermatozoa qualitues, one of which ismotility of spermatozoa. The motility of spermatozoa is very important to note in the development of artificial insemination techniques, because the success in artificial insemination techniques is strongly influenced by the motility of spermatozoa. The motility of semen spermatozoa is known through the process of examining the movement of spermatozoa in microscopic testing. The aim of this research was find out the effect of ejaculation fraction on motility of PE goat spermatozoa. Using an experimental method with a group's random consisting of two treatment groups; K1 is fraction 1and K2 is a fraction 2. The Replication of each treatment was eight replications and a total of sample research was 16 unit. The semen was collected once a week using artificial vagina (VA) technique. The semen collecting until the second ejaculation and  motility test of spermatozoa were immediately measured use sperm vision analyzers IVOS II. The test results of the ejaculation fraction observation using an independent sample t-test showed the value of t hit = 0.700 and t (0.025) than 2.14 t hit <t (0.025) meaning that H0 was received, there was no significant ejaculation fraction 1 with ejaculatory fraction 2; motility meaning of the spermatozoa from the PE goat of a fraction 1 and faction 2 not different significantly.
Keywords: ejaculation fraction, motility of Spermatozoa, PE goat.
ABSTRAK
Fraksi Ejakulasi pada Kambing Peranakan Etawa (PE) mempunyai kualitas spermatozoa dengan berbagai standart, salah satunya motilitas spermatozoa. Motilitas Spermatozoa menjadi hal yang sangat penting untuk diperhatikan dalam pengembangan teknik Inseminasi Buatan, karena keberhasilan dalam teknik Inseminasi Buatan sangat dipengaruhi oleh motilitas yang dimiliki spermatozoa. Motilitas spermatozoa semen diketahui melalui proses pemeriksaan daya gerak spermatozoa dalam uji mikroskopis. Penelitian ini bertujuan untuk mengetahui pengaruh fraksi ejakulasi terhadap motilitas spermatozoa kambing PE. Menggunakan metode percobaan dengan rancangan acak kelompok (RAK)  terdiri atas 2 kelompok perlakuan yakni Kelompok 1 (K1) adalah fraksi 1dan kelompok 2 (K2) adalah fraksi 2, ulangan masing-masing perlakuan 8 ulangan dan unit penelitian berjumlah 16. Penampungan semen dilakukan satu kali seminggu dengan teknik AV (Artificial Vagina). Penampungan dilakukan sampai ejakulat kedua. Segera setelah penampungan dilakukan pengukuran uji motilitas spermatozoa menggunakan sperm vision analyser IVOS II. Hasil uji pengamatan fraksi ejakulasi menggunakan t-test independent sample  menunjukkan nilai t hit= 0,700 dan t (0,025)= 2,14 maka t hit < t (0,025) artinya H0 di terima, yang mempunyai arti motilitas spermatozoa kambing PE dari fraksi 1 dan fraksi 2 tidak berbeda nyata.
Kata kunci: fraksi ejakulasi, motilitas spermatozoa, kambing PE
Differential responses in anti-predation traits of the native oyster Ostrea edulis and invasive Magallana gigas to ocean acidification and warming
Authors: Lemasson AJ , Knights AM
Published: March 2021

Ocean acidification and warming (OAW) pose a threat to marine organisms, with particular negative effects on molluscs, and can jeopardize the provision of associated ecosystem services. As predation is an important factor shaping populations in the marine environment, the ability of organisms to retain traits valuable in predation resistance under OAW may be decisive for future population maintenance. We examine how exposure to seawater temperature (control: 16.8°C and warm: 20°C) and atmosphericpCO2(ambient [~400], ~750, and ~1000 ppm) conditions affects traits linked to predation resistance (adductor muscle strength and shell strength) in two ecologically and economically important species of oysters (Magallana gigasandOstrea edulis) and relate them to changes in morphometry and fitness (condition index, muscle and shell metrics). We show thatO. edulisremained unimpacted following exposure to OAW scenarios. In contrast, the adductor muscle ofM. gigaswas 52% stronger under elevated temperature and ~750 ppmpCO2, and its shell was 44% weaker under combined elevated temperature and ~1000 ppmpCO2.This suggests greater resistance to mechanical predation toward the mid-21stcentury, but greater susceptibility toward the end of the century. For both species, individuals with more somatic tissue held an ecological advantage against predators; consequently, smaller oysters may be favoured by predators under OAW. By affecting fitness and predation resistance, OAW may be expected to induce shifts in predator-prey interactions and reshape assemblage structure due to species and size selection, which may consequently modify oyster reef functioning. This could in turn have implications for the provision of associated ecosystem services.
Videojugar. Estado del arte
Authors: Orozco Yenifer Andrea , Rojas Michael Andrés , Quimbaya Jorge Enrique
Published: March 2021

Las investigaciones sobre videojuegos cada vez cobran mayor relevancia en el campo de la psicología, su influencia en la estructura cultural de los escenarios digitales es relevante. Este estado del arte tiene como objetivo analizar los cambios que han tenido los estudios realizados desde el campo de la psicología alrededor de videojugar y las apuestas a generar pesquisas desde la cognición social.
Video comunitario colombiano en YouTube. Tipos de contenido y duración
Authors: Navarro Sánchez Gloria Esperanza
Published: March 2021

El artículo publica los resultados de la investigación que permitió establecer las características de 513 videos publicados en diez canales de YouTube creados por grupos colombianos dedicados a la producción de audiovisual comunitario o participativo, y clasificarlos según su contenido audiovisual y su duración. Se utilizó la metodología mixta y la técnica de recolección de la información fue el análisis de contenido y de datos descriptivos. Los canales objeto de estudio fueron: Kucha Suto Colectivo, Fundación Cine a la Calle, Red Comunicaciones Wayuu, ciudadcomuna, indigenasantioquia, SabaloIN, Fundación Cultural Ojo de Agua, de Fundescodes, Consejo Regional Indígena del Huila-Crihu, y ADCampesino. Los hallazgos muestran que en estos canales se publican videos de una gran variedad de categorías entre las que se destacan los documentales, los publicitarios así como los minimetrajes y cortometrajes. Entre usuarios o emirecs se advierte la tendencia a publicar y ver videos breves.
Luis Miguel de Zulategui: 1920 – 1970. Un legado colombo-español evocado desde su archivo musical
Authors: Flórez Cortés Carla Cristina
Published: March 2021

Este artículo de reflexión resultado de investigación, aborda la vida y obra de Luis Miguel de Zulategui, músico vasco exiliado en Colombia. Se valoró su archivo personal (1934-1970) el cual está custodiado por la Universidad Eafit. Sus aportes musicológicos y pedagógicos han repercutido en varias generaciones de músicos colombianos. La información se recopiló mediante tipologías documentales, con lo que se demuestra que la archivística se convirtió en una ciencia intermediaria entre la historia y la musicología en la reconstrucción de una historia de vida.
Autismo caminhos para a inclusão
Authors: Gikovate Carla , Mousinho Renata , Carvalho Isamara , dos Santos Caminha Vera Lúcia Prudência , Mendes Madureira Daniele Quintella , Salvini Rogério , Airão Barboza Ana Rosa , Wolff Mota Ana Carolina , Vieira Mauro Luís , dos Santos Stolfi Sabrina Vargas , Soares Martins Camila , de Figueiredo Walter Cátia Crivelenti , da Silva Amaral Felipe Vieira , Santos de Oliveira Edinea Dianne , Coelho de Moraes Cláudia , dos Santos Claudiana Prudência , Serra Dayse , Nascimento Silva Stefhanny Paulimineytrick , d’Oliveira de Paula Nunes Leila Regina , Lopes Araújo Maria Gabriela , Bejarano Gómez Alejandro , Da Silva Machado Bruno , de Oliveira Caminha Adriano , Silva Araújo Gisele , Seabra Junior Manoel Osmar
Published: March 2021

Este livro é o resultado do IV Simpósio sobre Ambientes Digitais de Aprendizagem para Crianças Autistas (IV SAACA), evento bianual realizado pela Universidade Federal Fluminense campus Aterrado em Volta Redonda – RJ, que, em sua quarta edição, reuniu profissionais que trabalham com a temática do Autismo e possuem estudos e práticas de relevância na área. Como resultado desse trabalho, nosso interesse em partilhar suas perspectivas, estudos e saberes através deste livro, visa a alcançar um maior número de pessoas que se interessam pelo tema.
Mechanistic Target of Rapamycin Inhibitors in Renal Cell Carcinoma: Potential, Limitations, and Perspectives
Authors: Faes Seraina , Demartines Nicolas , Dormond Olivier
Published: March 2021

Several elements highlight the importance of the mechanistic target of rapamycin (mTOR) in the biology of renal cell carcinoma (RCC). mTOR signaling pathway is indeed frequently activated in RCC, inducing cancer cell proliferation and survival. In addition, mTOR promotes tumor angiogenesis and regulates the expression of hypoxia-inducible factors that play an important role in a subset of RCC. Despite mTOR protumorigenic effects, mTOR inhibitors have failed to provide long-lasting anticancer benefits in RCC patients, highlighting the need to readdress their role in the treatment of RCC. This review aims to present the rationale and limitations of targeting mTOR in RCC. Future roles of mTOR inhibitors in the treatment of RCC are also discussed, in particular in the context of immunotherapies.
LRH1 Acts as an Oncogenic Driver in Human Osteosarcoma and Pan-Cancer
Authors: Song Yang , An Weiwei , Wang Hongmei , Gao Yuanren , Han Jihua , Hao Chenguang , Chen Lin , Liu Shilong , Xing Ying
Published: March 2021

Osteosarcoma (OS) that mainly occurs during childhood and adolescence is a devastating disease with poor prognosis presented by extreme metastases. Recent studies have revealed that liver receptor homolog 1 (LRH-1) plays a vital role in the metastasis of several human cancers, but its role is unknown in the metastasis of OS. In this study, Gene Ontology (GO) enrichment analyses based on high-throughput RNA-seq data revealed that LRH-1 acted a pivotal part in the positive regulation of cell migration, motility, and angiogenesis. Consistently, LRH-1 knockdown inhibited the migration of human OS cells, which was concurrent with the downregulation of mesenchymal markers and the upregulation of epithelial markers. In addition, short hairpin RNAs (shRNAs) targeting LRH-1 inactivated transforming growth factor beta (TGF-β) signaling pathway. LRH-1 knockdown inhibited human umbilical vein endothelial cell (HUVEC) proliferation, migration, and tube formation. Vascular endothelial growth factor A (VEGFA) expression was also downregulated after LRH-1 knockdown. Immunohistochemistry (IHC) revealed that the expression of LRH-1 protein was significantly higher in tumor tissues than in normal bone tissues. We found that high LRH-1 expression was associated with poor differentiation and advanced TNM stage in OS patients using IHC. Based on The Cancer Genome Atlas (TCGA) database, high LRH-1 expression predicts poor survival in lung squamous cell carcinoma (LUSC), kidney renal papillary cell carcinoma (KIRP), and pancreatic adenocarcinoma (PAAD). The downregulation of LRH-1 significantly hindered the migration and motility of LUSC cells. Using multi-omic bioinformatics, the positive correlation between LRH-1- and EMT-related genes was found across these three cancer types. GO analysis indicated that LRH-1 played a vital role in “blood vessel morphogenesis” or “vasculogenesis” in KIRP. Our results indicated that LRH-1 plays a tumor-promoting role in human OS, could predict the early metastatic potential, and may serve as a potential target for cancer therapy.
The m6A Reader YTHDF1 Facilitates the Tumorigenesis and Metastasis of Gastric Cancer via USP14 Translation in an m6A-Dependent Manner
Authors: Chen Xiao-Yu , Liang Rui , Yi You-Cai , Fan Hui-Ning , Chen Ming , Zhang Jing , Zhu Jin-Shui
Published: March 2021

Objectives

N6-methyladenosine (m6A) RNA methylation is implicated in the progression of multiple cancers via influencing mRNA modification. YTHDF1 can act as an oncogene in gastric cancer (GC), while the biological mechanisms via which YTHDF1 regulates gastric tumorigenesis through m6A modification remain largely unknown.

Methods

GEO and TCGA cohorts were analyzed for differentially expressed m6A modification components in GC clinical specimens and their association with clinical prognosis. Transwell and flow cytometry assays as well as subcutaneous xenograft and lung metastasis models were used to evaluate the phenotype of YTHDF1 in GC. Intersection of RNA/MeRIP-seq, luciferase assay, RIP-PCR, RNA pull-down and MeRIP-PCR was used to identify YTHDF1- modified USP14 and its m6A levels in GC cells.

Results

High-expressed YTHDF1 was found in GC tissues and was related to poor prognosis, acting as an independent prognostic factor of poor survival in GC patients. YTHDF1 deficiency inhibited cell proliferation and invasion (in vitro), and gastric tumorigenesis and lung metastasis (in vivo) and also induced cell apoptosis. Intersection assays revealed that YTHDF1 promoted USP14 protein translation in an m6A-dependent manner. USP14 upregulation was positively correlated with YTHDF1 expression and indicated a poor prognosis in GC.

Conclusion

Our data suggested that m6A reader YTHDF1 facilitated tumorigenesis and metastasis of GC by promoting USP14 protein translation in an m6A-dependent manner and might provide a potential target for GC treatment.
Which Hyperglycemic Model of Zebrafish (Danio rerio) Suites My Type 2 Diabetes Mellitus Research? A Scoring System for Available Methods
Authors: Salehpour Aria , Rezaei Mohammad , Khoradmehr Arezoo , Tahamtani Yaser , Tamadon Amin
Published: March 2021

Despite extensive studies on type 2 diabetes mellitus (T2DM), there is no definitive cure, drug, or prevention. Therefore, for developing new therapeutics, proper study models of T2DM is necessary to conduct further preclinical researches. Diabetes has been induced in animals using chemical, genetic, hormonal, antibody, viral, and surgical methods or a combination of them. Beside different approaches of diabetes induction, different animal species have been suggested. Although more than 85% of articles have proposed rat (genusRattus) as the proper model for diabetes induction, zebrafish (Danio rerio) models of diabetes are being used more frequently in diabetes related studies. In this systematic review, we compare different aspects of available methods of inducing hyperglycemia referred as T2DM in zebrafish by utilizing a scoring system. Evaluating 26 approved models of T2DM in zebrafish, this scoring system may help researchers to compare different T2DM zebrafish models and select the best one regarding their own research theme. Eventually, glyoxalase1 (glo1−/−) knockout model of hyperglycemia achieved the highest score. In addition to assessment of hyperglycemic induction methods in zebrafish, eight most commonly proposed diabetic induction approval methods are suggested to help researchers confirm their subsequent proposed models.
Cardiovascular Disease and COVID-19: Insight From Cases With Heart Failure
Authors: Yi Yang , Xu Yanan , Jiang Haibing , Wang Jun
Published: March 2021

Recent evidence indicates that a large proportion of deaths from coronavirus disease 2019 (COVID-19) can be attributed to cardiovascular disease, including acute myocardial infarction, arrhythmias and heart failure. Indeed, severe infection increases the risk of heart failure among patients with COVID-19. In most patients, heart failure arises from complex interactions between pre-existing conditions, cardiac injury, renin-angiotensin system activation, and the effects of systemic inflammation on the cardiovascular system. In this review, we summarize current knowledge regarding pathogen-driven heart failure occurring during treatment for COVID-19, the potential effects of commonly used cardiovascular and anti-infective drugs in these patients, and possible directions for establishing a theoretical basis for clinical treatment.