bluuebunny/crossref_metadata_2025 huggingface dataset:
A Retrospective Clinical Analysis of the Serum Bile Acid Alteration Caused by Traumatic Brain Injury
Authors: Zhu Yuanrun , Chen Zijian , You Wendong , Wang Yadong , Tu Mengdi , Zheng Peidong , Wen Liang , Yang Xiaofeng
Published: August 2021

Traumatic brain injury (TBI) can cause damage to peripheral organ systems, such as digestive organ system, and alterations of gut microbiota in addition to brain injury. Our previous study found that TBI induced gastrointestinal dysfunction accompanied by alterations of bile acid metabolism. Bile acid and its receptors have been reported to play important roles in various neurological diseases. To further examine the changes of bile acid metabolism in TBI patients, we performed a retrospective clinical analysis. In this study, 177 patients were included, and the results showed that TBI patients had more frequent antibiotic use compared with a control group. Regression analysis identified TBI as an independent factor for reduction of serum bile acid level (B = −1.762, p = 0.006), even with antibiotic use taken into a regression model. Sub-group regression analysis of TBI patients showed that antibiotic use was negatively associated with bile acid level, while creatinine and triglyceride were positively associated with bile acid level. In conclusion, these data indicated that TBI could greatly reduce serum bile acid. This study provided preliminary but novel clinical evidence of TBI interfering with bile acid metabolism, and further studies with large sample sizes are needed to validate these findings in the future.
Altered Cerebellar Resting-State Functional Connectivity in Early-Stage Parkinson's Disease Patients With Cognitive Impairment
Authors: Dan Xiaojuan , Hu Yang , Sun Junyan , Gao Linlin , Zhou Yongtao , Ma Jinghong , Doyon Julien , Wu Tao , Chan Piu
Published: August 2021

Background: Cognitive impairment is one of the most prominent non-motor symptoms in Parkinson's disease (PD), due in part to known cerebellar dysfunctions. Furthermore, previous studies have reported altered cerebellar functional connectivity (FC) in PD patients. Yet whether these changes are also due to the cognitive deficits in PD remain unclear.

Methods: A total of 122 non-dementia participants, including 64 patients with early PD and 58 age- and gender-matched elderly controls were stratified into four groups based on their cognitive status (normal cognition vs. cognitive impairment). Cerebellar volumetry and FC were investigated by analyzing, respectively, structural and resting-state functional MRI data among groups using quality control and quantitative measures. Correlation analysis between MRI metrics and clinical features (motor and cognitive scores) were performed.

Results: Compared to healthy control subjects with no cognitive deficits, altered cerebellar FC were observed in early PD participants with both motor and cognitive deficits, but not in PD patients with normal cognition, nor elderly subjects showing signs of a cognitive impairment. Moreover, connectivity between the “motor” cerebellum and SMA was positively correlated with motor scores, while intracerebellar connectivity was positively correlated with cognitive scores in PD patients with cognitive impairment. No cerebellar volumetric difference was observed between groups.

Conclusions: These findings show that altered cerebellar FC during resting state in early PD patients may be driven not solely by the motor deficits, but by cognitive deficits as well, hence highlighting the interplay between motor and cognitive functioning, and possibly reflecting compensatory mechanisms, in the early PD.
Mediators of Neuropathic Pain; Focus on Spinal Microglia, CSF-1, BDNF, CCL21, TNF-α, Wnt Ligands, and Interleukin 1β
Authors: Boakye Paul A. , Tang Shao-Jun , Smith Peter A.
Published: August 2021

Intractable neuropathic pain is a frequent consequence of nerve injury or disease. When peripheral nerves are injured, damaged axons undergo Wallerian degeneration. Schwann cells, mast cells, fibroblasts, keratinocytes and epithelial cells are activated leading to the generation of an “inflammatory soup” containing cytokines, chemokines and growth factors. These primary mediators sensitize sensory nerve endings, attract macrophages, neutrophils and lymphocytes, alter gene expression, promote post-translational modification of proteins, and alter ion channel function in primary afferent neurons. This leads to increased excitability and spontaneous activity and the generation of secondary mediators including colony stimulating factor 1 (CSF-1), chemokine C-C motif ligand 21 (CCL-21), Wnt3a, and Wnt5a. Release of these mediators from primary afferent neurons alters the properties of spinal microglial cells causing them to release tertiary mediators, in many situations via ATP-dependent mechanisms. Tertiary mediators such as BDNF, tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and other Wnt ligands facilitate the generation and transmission of nociceptive information by increasing excitatory glutamatergic transmission and attenuating inhibitory GABA and glycinergic transmission in the spinal dorsal horn. This review focusses on activation of microglia by secondary mediators, release of tertiary mediators from microglia and a description of their actions in the spinal dorsal horn. Attention is drawn to the substantial differences in the precise roles of various mediators in males compared to females. At least 25 different mediators have been identified but the similarity of their actions at sensory nerve endings, in the dorsal root ganglia and in the spinal cord means there is considerable redundancy in the available mechanisms. Despite this, behavioral studies show that interruption of the actions of any single mediator can relieve signs of pain in experimental animals. We draw attention this paradox. It is difficult to explain how inactivation of one mediator can relieve pain when so many parallel pathways are available.
Childhood Intra-Thoracic Tuberculosis Clinical Presentation Determines Yield of Laboratory Diagnostic Assays
Authors: Singh Urvashi B. , Verma Yogita , Jain Rakhi , Mukherjee Aparna , Gautam Hitender , Lodha Rakesh , Kabra Sushil K.
Published: August 2021

Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of the disease, the challenge in collecting appropriate specimens, and the low sensitivity of smear microscopy and culture. Culture and Xpert MTB/RIF provide higher diagnostic yield in presumptive TB in adults than in children. Current study was designed to understand poor yield of diagnostic assays in children. Children with presumptive ITTB were subjected to gastric aspirates and induced sputum twice. Samples were tested by Ziehl-Neelsen stain, Xpert MTB/RIF-assay, and MGIT-960 culture. Subjects were grouped as Confirmed, Unconfirmed, and Unlikely TB, and classified as progressive primary disease (PPD, lung parenchymal lesion), and primary pulmonary complex (PPC, hilar lymphadenopathy) on chest X-ray. Of children with culture-positive TB 51/394 (12.9%), culture-negative TB 305 (77.4%), and unlikely TB 38 (9.6%), 9 (2.3%) were smear positive, while 95 (24.1%) were Xpert-MTB/RIF positive. Xpert-MTB/RIF detected 40/51 culture confirmed cases (sensitivity 78.4% and NPV 96.3%). Culture was positive in more children presenting as PPD (p < 0.04). In culture-negative TB group, Xpert positivity was seen in 31% of those with PPD and 11.9% in those with PPC (p < 0.001).

Conclusion: Xpert-MTB/RIF improved diagnosis by 2-fold and increased detection of MDR-TB. Both liquid culture and Xpert-MTB/RIF gave higher yield in children with lung parenchymal lesions. Children with hilar lymphadenopathy without active lung parenchymal lesions had poor diagnostic yield even with sensitive nucleic acid amplification tests, due to paucibacillary/localized disease, suggesting possible utility of invasively collected samples in early diagnosis and treatment.
Burned-Out Testicular Tumors in Adolescents: Clinical Aspects and Outcome
Authors: Persano Giorgio , Crocoli Alessandro , De Pasquale Maria Debora , Cozza Raffaele , Alaggio Rita , Diomedi Camassei Francesca , Beati Federico , Di Paolo Pierluigi , Martucci Cristina , Inserra Alessandro
Published: August 2021

Purpose: Testicular germ cell tumors are the fourth most common neoplasm in adolescents, accounting for 8% of all tumors in the age group 15–19 years. On rare instances, the primary testicular lesion is not clinically or radiologically evident while nodal or visceral metastases represent the clinical manifestations of the disease. This phenomenon is described as “burned-out testicular tumor.” In this paper, the authors report a single-institution experience with burned-out testicular tumors in adolescents and discuss their clinical implications.

Patients and Methods: All the patients diagnosed with metastatic testicular germ cell tumors at Bambino Gesù Children Hospital between January 1, 2010, and June 30, 2020, were included in the study. Patients were categorized into two groups: “primary testicular” and “burned out.” All the patients were staged and treated according to the AIEOP–TCGM 2004 protocol.

Results: Eleven patients were classified as “primary testicular,” and five patients were classified as “burned out.” “Burned-out” tumors were associated with the presence of systemic symptoms compared to “primary testicular” tumors (80 vs. 0%; p = 0.0027) and higher aFP, hCG, and LDH levels (p < 0.00001). The “burned-out” population had a statistically significant higher incidence of relevant toxicity than the “primary testicular” population (80 vs. 18%; p = 0.0357) and a worse outcome in terms of both mean overall survival (15 vs. 43 months; p = 0.0299) and mean event-free survival (12 vs. 38 months; p = 0.0164).

Conclusion: “Burned-out” testicular tumors seem to be a well-distinct clinical entity with a high treatment-related toxicity and poor prognosis. Further studies are needed to clarify the “burned-out phenomenon” and to identify more effective therapeutic strategies for these patients.
EUS in Pediatrics: A Multicenter Experience and Review
Authors: Piester Travis L. , Liu Quin Y.
Published: August 2021

Background/Aim: Endoscopic ultrasound (EUS) is a well-established tool used in the evaluation and treatment of a wide range of pathologies in adult medicine. EUS in pediatrics has been shown to be safe and technically effective, and its use continues to evolve. This article aims to describe the EUS experience at our tertiary-care centers with regard to safety, technical success, and its impact in clinical management. We also discuss the current and developing diagnostic and therapeutic uses for EUS in pediatrics such as in pancreaticobiliary disease, congenital anomalies, eosinophilic esophagitis, inflammatory bowel disease, and liver disease.

Methods: This is a retrospective review of EUS performed by two pediatric gastroenterologists trained as endosonographers between April 2017 and November 2020. Patient demographics, procedure indication, procedure characteristics, technical success, and complications were collected. Literature review was performed to describe current and future uses of EUS in pediatrics.

Results: Ninety-eight EUS were performed with 15 (15.3%) including fine needle aspiration/biopsy and 9 (9.2%) cases being therapeutic. Most common indications include choledocholithiasis (n = 31, 31.6%), pancreatic fluid collections (n = 18, 18.4%), chronic and acute recurrent pancreatitis (n = 14, 14.3%), and acute pancreatitis characterization (n = 13, 13.3%). Notable indications of pancreatic mass (n = 6, 6.1%) and luminal lesions/strictures (n = 6, 6.1%) were less common. Complications were limited with one instance of questionable GI bleeding after cystgastrostomy creation. Ninety-eight of 98 (100%) cases were technically successful.

Conclusion/Discussion: EUS has been shown to be performed safely and successfully in the pediatric population by pediatric endosonographers. This study and review support its use in pediatric practice and demonstrate the wide variety of indications for EUS such as pancreatic cystgastrostomy, celiac plexus neurolysis, and evaluation of chronic pancreatitis. This literature review also demonstrates areas of potential development for EUS within the practice of pediatric gastroenterology.
Applicability of Quality Indicators for Appropriate Antibiotic use in Outpatient Parenteral Antimicrobial Therapy (OPAT): A Point Prevalence Survey
Authors: March-López Pablo , Freixa Inés Arancibia , Gil Mireia Martinez , Espinoza Gastón Araujo , Polonio Lidia Ortega , Paredes Elisabeth Cecilia , Sanchez Montserrat Carrasco , Sangrador Cristina , Pardo Júlia , Nicolás Jordi , Calbo Esther
Published: August 2021

The ability to measure the quality of antibiotic prescription is a critical element in any antimicrobial stewardship programme. The aim of this study was to evaluate the clinimetric properties of 33 quality indicators (QIs) developed to assess Outpatient Parenteral Antimicrobial Therapy (OPAT) and to identify potential room for improvement in a hospital-at-home (HaH) unit. Study performed in a healthcare district in Barcelona, Spain with 260,657 inhabitants, nine primary healthcare centres, a 400-bed acute care teaching hospital, and an HaH unit. We studied 33 QIs on appropriate antibiotic use and classified them as qualitative or quantitative. Quantitative QIs were further categorized as measurable or non-measurable depending on the availability of data in the patients’ medical records. Data from 202 OPAT episodes in 192 patients were assessed. Adherence was found for 22 of the 24 qualitative QIs analyzed; the other two showed room for improvement. Four of the nine quantitative indicators were non-measurable. High adherence rates were achieved for QI-17 “The OPAT plan should be documented” (84.65%), QI-26 “The OPAT treatment plan should include choice, dose, frequency, duration and follow-up plan” (79.70%), and QI-33 “The team should document clinical response” (94.55%). Adverse events were documented in just 1.98% of cases (QI-32) and 92.57% patients were classified as alive on discharge (QI-24). The QIs evaluated were applicable to clinical practice and proved useful for identifying areas with room for improvement in our setting and for guiding the design of future interventions with specific objectives.
Olive Oil: Nutritional Applications, Beneficial Health Aspects and its Prospective Application in Poultry Production
Authors: Bilal Rana M. , Liu Chunjie , Zhao Haohan , Wang Yanzhou , Farag Mayada R. , Alagawany Mahmoud , Hassan Faiz-ul , Elnesr Shaaban S. , Elwan Hamada A. M. , Qiu Huajiao , Lin Qian
Published: August 2021

Plant polyphenols have promoting health features, including anti-mutagenic, anti-inflammatory, anti-thrombotic, anti-atherogenic, and anti-allergic effects. These polyphenols improve the immune system by affecting the white blood cell proliferation, as well as by the synthesis of cytokines and other factors, which contribute to immunological resistance. Olive trees are one of the most famous trees in the world. Whereas, olive olive oil and derivatives represent a large group of feeding resource for farm animals. In recent years, remarkable studies have been carried out to show the possible use of olive oil and derivatives for improvement of both animal performance and product quality. In vivo application of olive oil and its derived products has shown to maintain oxidative balance owing to its polyphenolic content. Consumption of extra virgin olive oil reduces the inflammation, limits the risk of liver damage, and prevents the progression of steatohepatitis through its potent antioxidant activities. Also, the monounsaturated fatty acids content of olive oil (particularly oleic acid), might have positive impacts on lipid peroxidation and hepatic protection. Therefore, this review article aims to highlight the nutritional applications and beneficial health aspects of olive oil and its effect on poultry production.
The Peregrine Breather on the Zero-Background Limit as the Two-Soliton Degenerate Solution: An Experimental Study
Authors: Chabchoub Amin , Slunyaev Alexey , Hoffmann Norbert , Dias Frederic , Kibler Bertrand , Genty Goëry , Dudley John M. , Akhmediev Nail
Published: August 2021

Solitons are coherent structures that describe the nonlinear evolution of wave localizations in hydrodynamics, optics, plasma and Bose-Einstein condensates. While the Peregrine breather is known to amplify a single localized perturbation of a carrier wave of finite amplitude by a factor of three, there is a counterpart solution on zero background known as the degenerate two-soliton which also leads to high amplitude maxima. In this study, we report several observations of such multi-soliton with doubly-localized peaks in a water wave flume. The data collected in this experiment confirm the distinctive attainment of wave amplification by a factor of two in good agreement with the dynamics of the nonlinear Schrödinger equation solution. Advanced numerical simulations solving the problem of nonlinear free water surface boundary conditions of an ideal fluid quantify the physical limitations of the degenerate two-soliton in hydrodynamics.
Head-Down-Tilt Bed Rest With Elevated CO2: Effects of a Pilot Spaceflight Analog on Neural Function and Performance During a Cognitive-Motor Dual Task
Authors: Mahadevan Aditya D. , Hupfeld Kathleen E. , Lee Jessica K. , De Dios Yiri E. , Kofman Igor S. , Beltran Nichole E. , Mulder Edwin , Bloomberg Jacob J. , Mulavara Ajitkumar P. , Seidler Rachael D.
Published: August 2021

Spaceflight has widespread effects on human performance, including on the ability to dual task. Here, we examine how a spaceflight analog comprising 30 days of head-down-tilt bed rest (HDBR) combined with 0.5% ambient CO2 (HDBR + CO2) influences performance and functional activity of the brain during single and dual tasking of a cognitive and a motor task. The addition of CO2 to HDBR is thought to better mimic the conditions aboard the International Space Station. Participants completed three tasks: (1) COUNT: counting the number of times an oddball stimulus was presented among distractors; (2) TAP: tapping one of two buttons in response to a visual cue; and (3) DUAL: performing both tasks concurrently. Eleven participants (six males) underwent functional MRI (fMRI) while performing these tasks at six time points: twice before HDBR + CO2, twice during HDBR + CO2, and twice after HDBR + CO2. Behavioral measures included reaction time, standard error of reaction time, and tapping accuracy during the TAP and DUAL tasks, and the dual task cost (DTCost) of each of these measures. We also quantified DTCost of fMRI brain activation. In our previous HDBR study of 13 participants (with atmospheric CO2), subjects experienced TAP accuracy improvements during bed rest, whereas TAP accuracy declined while in the current study of HDBR + CO2. In the HDBR + CO2 subjects, we identified a region in the superior frontal gyrus that showed decreased DTCost of brain activation while in HDBR + CO2, and recovered back to baseline levels before the completion of bed rest. Compared to HDBR alone, we found different patterns of brain activation change with HDBR + CO2. HDBR + CO2 subjects had increased DTCost in the middle temporal gyrus whereas HDBR subjects had decreased DTCost in the same area. Five of the HDBR + CO2 subjects developed signs of spaceflight-associated neuro-ocular syndrome (SANS). These subjects exhibited lower baseline dual task activation and higher slopes of change during HDBR + CO2 than subjects with no signs of SANS. Collectively, this pilot study provides insight into the additional and/or interactive effects of CO2 levels during HDBR, and information regarding the impacts of this spaceflight analog environment on the neural correlates of dual tasking.
A Novel Fragmentation Sensitivity Index Determines the Susceptibility of Red Blood Cells to Mechanical Trauma
Authors: Ugurel Elif , Goksel Evrim , Goktas Polat , Cilek Neslihan , Atar Dila , Yalcin Ozlem
Published: August 2021

Supraphysiological shear stresses (SSs) induce irreversible impairments of red blood cell (RBC) deformability, overstretching of RBC membrane, or fragmentation of RBCs that causes free hemoglobin to be released into plasma, which may lead to anemia. The magnitude and exposure tisme of the SSs are two critical parameters that determine the hemolytic threshold of a healthy RBC. However, impairments in the membrane stability of damaged cells reduce the hemolytic threshold and increase the susceptibility of the cell membrane to supraphysiological SSs, leading to cell fragmentation. The severity of the RBC fragmentation as a response to the mechanical damage and the critical SS levels causing fragmentation are not previously defined. In this study, we investigated the RBC mechanical damage in oxidative stress (OS) and metabolic depletion (MD) models by applying supraphysiological SSs up to 100 Pa by an ektacytometer (LORRCA MaxSis) and then assessed RBC deformability. Next, we examined hemolysis and measured RBC volume and count by Multisizer 3 Coulter Counter to evaluate RBC fragmentation. RBC deformability was significantly impaired in the range of 20–50 Pa in OS compared with healthy controls (p < 0.05). Hemolysis was detected at 90–100 Pa SS levels in MD and all applied SS levels in OS. Supraphysiological SSs increased RBC volume in both the damage models and the control group. The number of fragmented cells increased at 100 Pa SS in the control and MD and at all SS levels in OS, which was accompanied by hemolysis. Fragmentation sensitivity index increased at 50–100 Pa SS in the control, 100 Pa SS in MD, and at all SS levels in OS. Therefore, we propose RBC fragmentation as a novel sensitivity index for damaged RBCs experiencing a mechanical trauma before they undergo fragmentation. Our approach for the assessment of mechanical risk sensitivity by RBC fragmentation could facilitate the close monitoring of shear-mediated RBC response and provide an effective and accurate method for detecting RBC damage in mechanical circulatory assist devices used in routine clinical procedures.
Asymmetric Reproductive Barriers and Gene Flow Promote the Rise of a Stable Hybrid Zone in the Mediterranean High Mountain
Authors: Abdelaziz Mohamed , Muñoz-Pajares A. Jesús , Berbel Modesto , García-Muñoz Ana , Gómez José M. , Perfectti Francisco
Published: August 2021

Hybrid zones have the potential to shed light on evolutionary processes driving adaptation and speciation. Secondary contact hybrid zones are particularly powerful natural systems for studying the interaction between divergent genomes to understand the mode and rate at which reproductive isolation accumulates during speciation. We have studied a total of 720 plants belonging to five populations from twoErysimum(Brassicaceae) species presenting a contact zone in the Sierra Nevada mountains (SE Spain). The plants were phenotyped in 2007 and 2017, and most of them were genotyped the first year using 10 microsatellite markers. Plants coming from natural populations were grown in a common garden to evaluate the reproductive barriers between both species by means of controlled crosses. All the plants used for the field and greenhouse study were characterized by measuring traits related to plant size and flower size. We estimated the genetic molecular variances, the genetic differentiation, and the genetic structure by means of the F-statistic and Bayesian inference. We also estimated the amount of recent gene flow between populations. We found a narrow unimodal hybrid zone where the hybrid genotypes appear to have been maintained by significant levels of a unidirectional gene flow coming from parental populations and from weak reproductive isolation between them. Hybrid plants exhibited intermediate or vigorous phenotypes depending on the analyzed trait. The phenotypic differences between the hybrid and the parental plants were highly coherent between the field and controlled cross experiments and through time. The highly coherent results obtained by combining field, experimental, and genetic data demonstrate the existence of a stable and narrow unimodal hybrid zone betweenErysimum mediohispanicumandErysimum nevadenseat the high elevation of the Sierra Nevada mountains.
Characterizing Winter Wheat Germplasm for Fusarium Head Blight Resistance Under Accelerated Growth Conditions
Authors: Zakieh Mustafa , Gaikpa David S. , Leiva Sandoval Fernanda , Alamrani Marwan , Henriksson Tina , Odilbekov Firuz , Chawade Aakash
Published: August 2021

Fusarium head blight (FHB) is one of the economically important diseases of wheat as it causes severe yield loss and reduces grain quality. In winter wheat, due to its vernalization requirement, it takes an exceptionally long time for plants to reach the heading stage, thereby prolonging the time it takes for characterizing germplasm for FHB resistance. Therefore, in this work, we developed a protocol to evaluate winter wheat germplasm for FHB resistance under accelerated growth conditions. The protocol reduces the time required for plants to begin heading while avoiding any visible symptoms of stress on plants. The protocol was tested on 432 genotypes obtained from a breeding program and a genebank. The mean area under disease progress curve for FHB was 225.13 in the breeding set and 195.53 in the genebank set, indicating that the germplasm from the genebank set had higher resistance to FHB. In total, 10 quantitative trait loci (QTL) for FHB severity were identified by association mapping. Of these, nine QTL were identified in the combined set comprising both genebank and breeding sets, while two QTL each were identified in the breeding set and genebank set, respectively, when analyzed separately. Some QTLs overlapped between the three datasets. The results reveal that the protocol for FHB evaluation integrating accelerated growth conditions is an efficient approach for FHB resistance breeding in winter wheat and can be even applied to spring wheat after minor modifications.
Construction of Consensus Genetic Map With Applications in Gene Mapping of Wheat (Triticum aestivum L.) Using 90K SNP Array
Authors: Qu Pingping , Wang Jiankang , Wen Weie , Gao Fengmei , Liu Jindong , Xia Xianchun , Peng Huiru , Zhang Luyan
Published: August 2021

Wheat is one of the most important cereal crops worldwide. A consensus map combines genetic information from multiple populations, providing an effective alternative to improve the genome coverage and marker density. In this study, we constructed a consensus map from three populations of recombinant inbred lines (RILs) of wheat using a 90K single nucleotide polymorphism (SNP) array. Phenotypic data on plant height (PH), spike length (SL), and thousand-kernel weight (TKW) was collected in six, four, and four environments in the three populations, and then used for quantitative trait locus (QTL) mapping. The mapping results obtained using the constructed consensus map were compared with previous results obtained using individual maps and previous studies on other populations. A simulation experiment was also conducted to assess the performance of QTL mapping with the consensus map. The constructed consensus map from the three populations spanned 4558.55 cM in length, with 25,667 SNPs, having high collinearity with physical map and individual maps. Based on the consensus map, 21, 27, and 19 stable QTLs were identified for PH, SL, and TKW, much more than those detected with individual maps. Four PH QTLs and six SL QTLs were likely to be novel. A putative gene called TraesCS4D02G076400 encoding gibberellin-regulated protein was identified to be the candidate gene for one major PH QTL located on 4DS, which may enrich genetic resources in wheat semi-dwarfing breeding. The simulation results indicated that the length of the confidence interval and standard errors of the QTLs detected using the consensus map were much smaller than those detected using individual maps. The consensus map constructed in this study provides the underlying genetic information for systematic mapping, comparison, and clustering of QTL, and gene discovery in wheat genetic study. The QTLs detected in this study had stable effects across environments and can be used to improve the wide adaptation of wheat cultivars through marker-assisted breeding.
Psychological Construction and Cultivation of New Entrepreneurs Using Industrial Cluster Theory and Multidimensional Structure Model
Authors: Fan Huiling
Published: August 2021

This study aims to analyze the influencing factors and training plans of cultivating new entrepreneurs in the new era based on industrial cluster theory. First, the status of the research on the correlation between psychological characteristics and entrepreneurial performance of new entrepreneurs is reviewed. Second, 200 new entrepreneurs in the Xi’an City of Shaanxi Province are randomly selected as the research objects. An empirical model of the correlation between entrepreneurial psychological characteristics and entrepreneurial performance is innovatively proposed. Finally, the questionnaire data are analyzed by correlation analysis and SPSS 26.0 (Chicago) statistical analysis. The results show that most of the entrepreneurs are between 30 and 50 years old, with a larger number of mens, and most graduate from junior colleges. The gender and education level of the new entrepreneurs have a little impact on their entrepreneurial performance (P > 0.05), whereas their age has a significant impact on their entrepreneurial performance (P < 0.05). Moreover, there is a significant correlation between the psychological characteristics of the entrepreneur and the two dimensions of entrepreneurial performance (development and profitability) (P < 0.05). This shows that the key to cultivating new entrepreneurs is to develop their age and psychological characteristics, which can enhance the entrepreneurial ability of new entrepreneurs and provide a basis for cultivating multidimensional entrepreneurial talents in new enterprises.
Seroprevalence of Cytomegalovirus and Associated Factors Among Preconception Women: A Cross-Sectional Nationwide Study in China
Authors: Zhou Qiongjie , Wang Qiaomei , Shen Haiping , Zhang Yiping , Zhang Shikun , Li Xiaotian , Acharya Ganesh
Published: August 2021

Background: Cytomegalovirus seroconversion during pregnancy is common and has a substantial risk of congenital infection with longterm sequale. Screening during pregnancy or vaccination have not been shown to be effective for eliminating congenital infections. Preconception screening policy has not been evaluated adequately in a large scale. This nationwide study aimed to investigate epidemiological features of cytomegalovirus seropositivity and its geographic variation among Chinese women planning a pregnancy to gather epidemiological evidence as an essential for developing novel prevention strategies.

Method: This cross-sectional sero-epidemiological survey enrolled women intending to become pregnant within 6 months in mainland China during 2010–2012. The primary outcomes in this study were cytomegalovirus Immunoglobulin G and M seropositivity. Secondary outcomes were the associations between Immunoglobulin G and Immunoglobulin M, with socio-demographic characteristics, including age, occupation, education level, place of residence, and ethnicity. The overall seropositivity and regional disparity was analyzed on the individual and regional level, respectively.

Results: This study included data from 1,564,649 women from 31 provinces in mainland China. Among participants, 38.6% (n = 603,511) were cytomegalovirus immunoglobulin G+, 0.4% (n = 6,747) were immunoglobulin M+, and 0.2% (n = 2,879) were immunoglobulin M+ and immunoglobulin G+. On individual level, participant's age, ethnicity, and residing region were significantly associated with IgG+, IgM+, and IgM+IgG+ (P < 0.001), while occupation, education level, and place of residence were not statistically significant (P > 0.05). On regional level, cytomegalovirus immunoglobulin G and immunoglobulin M seropositivity was highest in the eastern region (49.5 and 0.5%, respectively), and lowest in the western region (26.9 and 0.4%, respectively). This geographic variation was also noted at the provincial level, characterized by higher provincial immunoglobulin M+ and immunoglobulin G+ rates associated with higher immunoglobulin G seropositivity. In the subgroup analysis of immunoglobulin G seropositivity, areas of higher immunoglobulin G positivity had a higher rate of immunoglobulin M+, indicating an expected increased risk of reinfection and primary infection.

Conclusions: A substantial proportion of women (>60%) were susceptible to cytomegalovirus in preconception period in China, and immunoglobulin G seropositivity was seen at a low-medium level with substantial geographic variation. Integration of cytomegalovirus antibody testing in preconception screening program based on regional immunoglobulin G seropositivity, should be considered to promote strategies directed toward preventing sero-conversion during pregnancy to reduce the risk of this congenital infection.
Cost-Effectiveness of Aprepitant in Preventing Chemotherapy-Induced Nausea and Vomiting: A Systematic Review of Published Articles
Authors: Qiu Tingting , Men Peng , Sun Tong , Zhai Suodi
Published: August 2021

Objectives: The aim of this systematic review is to assess the published cost-effectiveness analyses of aprepitant for patients with chemotherapy-induced nausea and vomiting (CINV).

Methods: A systematic literature search was performed on PubMed, EMbase, the Cochrane Library, CNKI, WANFANG DATA, and CBM database. The date of publication is up to January 2019. Two reviewers independently reviewed titles, abstracts, and articles sequentially to select studies for data abstraction based on the inclusion and exclusion criteria. Disagreements were resolved and reviewers reached a consensus. The quality of the included studies was assessed according to the 24-item checklist of the consolidated health economic evaluation reporting standards (CHEERS). The costs reported by the included studies were converted to US dollars via purchasing power parities (PPP) in the year 2019 using the CCEMG–EPPI–Certer Cost Converter.

Results: Thirteen articles were included based on the inclusion criteria for cost-effectiveness analysis and cost-utility analysis. Twelve studies were rated as good quality and one as a moderate quality based on the CHEERS checklist. Eight studies compared aprepitant plus 5-hydroxytryptamine-3 receptor antagonist (5-HT3RA) and dexamethasone with the standard regimen (5-HT3RA and dexamethasone). It was concluded that aprepitant plus standard regimen was a cost-effective strategy for preventing CINV. Only one study that compared aprepitant plus 5-HT3RA with 5-HT3RA, concluded that the addition of aprepitant reduced the incidence of severe nausea, and it might also provide an economic benefit in the overall management. Four studies that compared aprepitant with other antiemetic drugs concluded that aprepitant is a cost-effective strategy for preventing CINV compared with metoclopramide. However, netupitan + palonosetron and olanzapine are cost-effective compared with aprepitant.

Conclusion: This study is the first systematic evaluation of adding aprepitant to standard regimens for patients with CINV. Most economic evaluations of antiemetic medications are reported to be of good quality. Adding aprepitant to standard regimens is found to be a cost-effective strategy for preventing CINV.
Variations in COVID-19 Spread and Control Measures in the Palestinian Territories
Authors: Abed Yehia , Shaheen Amira , Abedrabbo Ali
Published: August 2021

Palestinians are facing the epidemic while they are the only occupied country globally, with around 2 million inhabitants under siege in the Gaza Strip (GS) for the last 14 years and have no control over the health of the Palestinians in East-Jerusalem (EJ). Such catastrophic situations created a variety in the spread of the COVID-19 pandemic in different territories. This study aimed to explore variation in COVID-19 spread, risk factors, and intervention activities in the three Palestinian territories: West Bank (WB), EJ, and GS to learn from the current gaps to overcome this pandemic and be prepared for future emergencies. Epidemiological data regarding COVID-19 were obtained from online websites, Palestinian national reports, WHO reports, and scientific publications. Morbidity and mortality indicators in Palestine are higher than the global level with rate variation in the three territories. COVID-19 incidence and mortality rates are higher in EJ and lowest in GS, while case fatalities are around 1% all over the country. Social gathering and lack of readiness of the fragmented health systems (there are two systems; Palestinian serves the WB and GS and Israeli serves the EJ) are risk factors in the three Palestinian territories. The most prominent risk in GS is overcrowding, while the movement of the workers inside Israel and travel are more prevalent in the WB and EJ. The WHO and international organizations play an active role in responding to a community spread, mainly national coordination, risk communication and community engagement, laboratory support, surveillance and procurement, and supply management. Recommendations include restructuring the national committees, reviewing and standardization of the national protocols, expanding infections prevention training, supporting and developing the capacity of laboratories, and setting the role of NGOs besides community engagement and participation.
Exploring Environmental Factors That Drive Diel Variations in Tree Water Storage Using Wavelet Analysis
Authors: Harmon Ryan E. , Barnard Holly R. , Day-Lewis Frederick D. , Mao Deqiang , Singha Kamini
Published: August 2021

Internal water storage within trees can be a critical reservoir that helps trees overcome both short- and long-duration environmental stresses. We monitored changes in internal tree water storage in a ponderosa pine on daily and seasonal scales using moisture probes, a dendrometer, and time-lapse electrical resistivity imaging (ERI). These data were used to investigate how patterns of in-tree water storage are affected by changes in sapflow rates, soil moisture, and meteorologic factors such as vapor pressure deficit. Measurements of xylem fluid electrical conductivity were constant in the early growing season while inverted sapwood electrical conductivity steadily increased, suggesting that increases in sapwood electrical conductivity did not result from an increase in xylem fluid electrical conductivity. Seasonal increases in stem electrical conductivity corresponded with seasonal increases in trunk diameter, suggesting that increased electrical conductivity may result from new growth. On the daily scale, changes in inverted sapwood electrical conductivity correspond to changes in sapwood moisture. Wavelet analyses indicated that lag times between inverted electrical conductivity and sapflow increased after storm events, suggesting that as soils wetted, reliance on internal water storage decreased, as did the time required to refill daily deficits in internal water storage. We found short time lags between sapflow and inverted electrical conductivity with dry conditions, when ponderosa pine are known to reduce stomatal conductance to avoid xylem cavitation. A decrease in diel amplitudes of inverted sapwood electrical conductivity during dry periods suggest that the ponderosa pine relied on internal water storage to supplement transpiration demands, but as drought conditions progressed, tree water storage contributions to transpiration decreased. Time-lapse ERI- and wavelet-analysis results highlight the important role internal tree water storage plays in supporting transpiration throughout a day and during periods of declining subsurface moisture.
The Impact of the COVID-19 Pandemic on Families: Young People’s Experiences in Estonia
Authors: Kutsar Dagmar , Kurvet-Käosaar Leena
Published: August 2021

This articles reflects the impacts of the COVID-19 pandemic on the everyday lives of children and their families in Estonia during lockdown in spring 2020 and 2021. The data corpus is based on diaries compiled by children during the first lockdown in 2020 for a collection at the Estonian Literary Museum, and on a series of semi-structured interviews with children documenting their experiences during lockdown in spring 2021. The study draws on literature from the “new sociology of childhood” and applies Bronfenbrenner’s social ecological model to an analysis of young people’s experiences when their mobility outside the home was restricted, and they were forced to reorganise their time use. The findings show how the pandemic extended the social contexts in which children and their families are embedded and highlighted the role played by socio-cultural factors in shaping children’s coping capacities. In combination, analysis of the two datasets demonstrated the differential effects of lockdown on young children. The accounts from the first wave of the pandemic in 2020 suggested that positive family environments could smooth the negative effects of lockdown and help them cope with unexpected changes in their everyday lives. The interviews during the second outbreak of the pandemic revealed how the emerging weariness and boredom reported by some children strained family relationships. The amount of time that children spent online both modified and expanded their experiences of technology-supported interactive spaces. Their reports showed that the interactive contexts in which they were operating through social media extended beyond national borders to an interest in transnational and global events. Online communication did not, however, compensate for the loss of real-life contacts with friends, which became a major concern for young people in Estonia. In the concluding discussion, the authors consider policy responses that address the main issues identified in the research.
Photobioreactor Limnospira indica Growth Model: Application From the MELiSSA Plant Pilot Scale to ISS Flight Experiment
Authors: Poughon Laurent , Creuly Catherine , Godia Francesc , Leys Natalie , Dussap Claude-Gilles
Published: August 2021

Bioregenerative life support systems (BLSS) are the foundation stone to self-sustainable manned space missions. The MELiSSA is a BLSS concept that has evolved through a mechanistic engineering approach designed to acquire both theoretical and technical knowledge on each subsystem independently and, therefore, produces the necessary knowledge and experience needed to co-integrate all the subsystems together with a high level of control. One of the subsystems is a photobioreactor colonized by an axenic culture of the cyanobacterium Limnospira indica PCC8005 for revitalizing the air for the crew. This subsystem was extensively studied, and a mass balanced mechanistic model was developed to describe, predict, and control the photobioreactor. The model was based on a light transfer limitation model coupled to a kinetic model for the cyanobacteria growth through a Linear Thermodynamics of Irreversible Processes (LTIP) approach, including substrate limitation. The model was integrated into several hydrodynamic models adapted to several photobioreactors design and experiments, from a 100 L airlift pilot scale ground photobioreactor to a 50 ml membrane photobioreactor for ISS flight. Through this article we detail the principles of this mechanistic model and their application to different photobioreactor scales for predictive and descriptive simulations.
Mesoscale Structure in the Solar Wind
Authors: Viall N. M. , DeForest C. E. , Kepko L.
Published: August 2021

Structures in the solar wind result from two basic mechanisms: structures injected or imposed directly by the Sun, and structures formed through processing en route as the solar wind advects outward and fills the heliosphere. On the largest scales, solar structures directly impose heliospheric structures, such as coronal holes imposing high speed streams of solar wind. Transient solar processes can inject large-scale structure directly into the heliosphere as well, such as coronal mass ejections. At the smallest, kinetic scales, the solar wind plasma continually evolves, converting energy into heat, and all structure at these scales is formed en route. “Mesoscale” structures, with scales at 1 AU in the approximate spatial range of 5–10,000 Mm and temporal range of 10 s–7 h, lie in the orders of magnitude gap between the two size-scale extremes. Structures of this size regime are created through both mechanisms. Competition between the imposed and injected structures with turbulent and other evolution leads to complex structuring and dynamics. The goal is to understand this interplay and to determine which type of mesoscale structures dominate the solar wind under which conditions. However, the mesoscale regime is also the region of observation space that is grossly under-sampled. The sparse in situ measurements that currently exist are only able to measure individual instances of discrete structures, and are not capable of following their evolution or spatial extent. Remote imaging has captured global and large scale features and their evolution, but does not yet have the sensitivity to measure most mesoscale structures and their evolution. Similarly, simulations cannot model the global system while simultaneously resolving kinetic effects. It is important to understand the source and evolution of solar wind mesoscale structures because they contain information on how the Sun forms the solar wind, and constrains the physics of turbulent processes. Mesoscale structures also comprise the ground state of space weather, continually buffeting planetary magnetospheres. In this paper we describe the current understanding of the formation and evolution mechanisms of mesoscale structures in the solar wind, their characteristics, implications, and future steps for research progress on this topic.
Difference in Personality Traits and Symptom Intensity According to the Trigger-Based Classification of Throwing Yips in Baseball Players
Authors: Aoyama Toshiyuki , Ae Kazumichi , Souma Hiroto , Miyata Kazuhiro , Kajita Kazuhiro , Kawamura Takashi , Iwai Koichi
Published: August 2021

The triggers of initial onset of yips symptoms can be broadly divided into psychological and non-psychological factors; however, a trigger-based classification of yips has not been established. This study aims to obtain insight into the prevention of yips by clarifying whether there are differences in symptoms and personality traits according to a trigger-based classification of yips in baseball players. A total of 107 college baseball players responded to a questionnaire assessing the presence or absence of yips and its symptoms. They were classified into the psychologically triggered yips group, the non-psychologically triggered yips group, and the non-yips group based on the presence or absence of yips and the triggers of its initial onset. Additionally, we compared whether personality traits examined by the NEO Five-Factor Inventory differed across these three groups. The psychologically triggered yips group had significantly higher agreeableness scores compared with the non-yips group, whereas the non-psychologically triggered yips group had significantly higher neuroticism scores compared with the psychologically triggered yips group. In the non-psychologically triggered yips group, there was a significantly higher frequency of throwing errors than in the psychologically triggered yips group, with a tendency to develop yips symptoms gradually. Since the trigger-based classification of yips is closely related to the strength of the yips symptoms and the players' personality traits, the results of this study contribute to a better understanding of the symptoms of yips and establishment of the prevention of yips. Large prospective studies are necessary to determine the causal relationship between a trigger-based classification of yips and the personality traits and symptoms of athletes with yips.