bluuebunny/crossref_metadata_2025 huggingface dataset:
From Lab to Market: Learning Entrepreneurial Marketing Through Multi-Semester, Stage-Gate, Capstone Project in STEM MBA
Authors: Marin Alejandra , Parvatiyar Atul , Mitchell Ronald K. , Villegas Dino
Published: July 2023

Entrepreneurs build successful businesses by taking innovative ideas from research labs to market. This article describes a pedagogical approach and its outcomes in utilizing a multi-stage, multi-course, and multi-semester capstone integrative project to teach entrepreneurial marketing (EM) of early-stage technologies. Herein we explain concepts and practices that enable the learning needed within STEM (Science, Technology, Engineering, Mathematics) MBA programs to commercialize and market products and services arising from new technologies. The pedagogy follows a four-course sequence that applies strategic frameworks and tools to develop opportunity space for a patented idea, develop the idea into product options, undertake customer discovery, build marketing and sales strategies, and translate opportunities into venture planning. These lab-to-market outcomes are accomplished by applying deliberate practice-based action learning methods to build students’ knowledge bases and problem-solving process skills that increase their entrepreneurial expertise. Through learner engagement in a sequence of innovative, data-driven, analytical processes that focus on identifying, validating, and strategizing around scalable new ideas, this pedagogy enables students to learn EM skills that can be applied to different industries and companies at various stages of development but with an emphasis on early-stage technologies.
Dynamic Effect of Fluid–Structure Interaction and Modal Analysis of Crude–Pipeline Interaction System Considering the Fluctuating Pressure of Medium
Authors: Weng Guangyuan , Xu Chenxi , Xie Qixuan , Wu Xiaomeng , Lan Guanqi , Zhang Yumin , Zhu Xiyu
Published: July 2023

This work explored the fluid–structure interaction (FSI) effect of crude oil and pipeline and the vibration characteristics of the pipe–crude oil structural system. A finite element computational analysis theory and corresponding simulation method were proposed for crude oil pipelines considering the FSI effect. Meanwhile, the spring model of the FSI interface between crude oil and pipeline was proposed. The experimental simulation and neural network were adopted to obtain the flow-excited pulsation pressure (FEPP), and the kinetic equations of the pipe–crude oil FSI were analyzed. The finite element computation (FEC) model for pipe–crude oil bi-directional FSI dynamics was established, which was composed of five support span lengths and four pipe liquid levels and can provide five different crude oil fluid velocities. One hundred orthogonal simulation test conditions were designed. In addition, the displacement response and acceleration response under FSI of the pipe wall were calculated, and the first six orders of inherent frequencies and main vibration patterns of the FEC model were analyzed. The results revealed that the dynamic effect of FSI between the crude oil transport medium and pipeline was complex and was influenced by the length of the pipeline crossing section, medium flow velocity, difference of liquid surface height, and displacement response. The acceleration response possessed prominent vibration decay characteristics, and the response peak in the same condition was about 8.2 times larger than that of the steady-flow state response. Therefore, the effect changed the inherent frequency and vibration pattern of the pipeline, to which the engineering design should pay sufficient attention.
An alginate-Based tube gel delivering 2-deoxy-D-ribose for stimulation of wound healing
Authors: Abid Safina , Zulfiqar Saima , Anjum Muhammad A , Bullock Anthony J , MacNeil Sheila , Yar Muhammad
Published: July 2023

Developing multifunctional wound dressings capable of inducing rapid angiogenesis and with antibacterial activity would be attractive for diabetic and superficial wound healing. Hydrogels delivered from tubes have several desirable features -they are easy to apply, keep the wound moist, reduce the entry of microorganisms and avoid the need for painful dressing removal. Previously we reported that 2 deoxy-D-ribose (2dDR) delivered from a variety of dressings is capable of promoting wound healing by stimulating angiogenesis. Alginate hydrogels are an ideal vehicle to deliver a bioactive agent capable of promoting wound healing. In this study we developed and evaluated a tube hydrogel capable of delivering 2dDR with the aim of achieving a stable, convenient to administer and biologically effective wound treatment. Further, we included the stabilizer 2-phenoxy ethanol which provided antimicrobial activity. We synthesized hydrogels by the Green method, using simple mixing of sodium alginate, propylene glycol, 2-phenoxy ethanol and 2dDR in water. FTIR (Fourier transformation infrared spectroscopy) analysis confirmed an absence of undesirable chemical changes in the gel components, and SEM images of the freeze-dried gels showed porous structures. When 2dDR alginate gel (2dDR-SA hydrogel) was placed in PBS at 37°C, almost 92% of 2dDR was released within 7 days. When tested on cultured cells, 2dDR-SA hydrogels did not inhibit metabolic activity or proliferation, achieving up to 90 and 98% of control respectively over 7 days. 2dDR-SA hydrogel also showed anti-bacterial activity against E. coli, Pseudomonas aeruginosa, Staphylococcus aureus, and MRSA which was attributable to the stabilizer 2-phenoxy ethanol in the hydrogel. Stimulation of angiogenesis in the chorioallantoic membrane assay by 2dDR-SA hydrogel was found to be significant compared to the blank-SA. Wound healing potential was studied in full-thickness wounds in rats where acceleration of wound healing was seen. H&E staining of the wound tissue showed an enhanced number of blood vessels and re-epithelization, and a reduced number of inflammatory cells in 2dDR-SA treated animals compared to blank-hydrogels while Masson’s trichrome staining showed increased collagen deposition. In summary we describe a convenient to apply hydrogel which has promise for use in a range of superficial skin wounds including applications in chronic wound care.
RCM adjustment for a double-parallelogram based RCM mechanism used for MIS robots
Authors: Singh Amanpreet , Khatait Jitendra P
Published: July 2023

The inevitable errors in the manufacturing, assembly, and testing of a remote center-of-motion (RCM) mechanism lead to the erroneous link parameters which deviate its geometry. Consequently, the motion, as well as the force, become non-zero at the entry port to the patient’s body which is undesirable. To address this issue, the current work first presents the accurate kinematic model of the RCM of an exemplary double-parallelogram-based RCM mechanism. Thereafter, it introduces the RCM adjustment concept and a flexure-based compliant mechanism to nullify/minimize the said deviation. For the analytical design of the flexure element, this paper makes the required design calculations and performs the finite element analysis. It is followed by the disclosure of the RCM Adjustment System and a systematized method of RCM adjustment for a mechanically constrained RCM device. The devised system is based on the prescribed compliant mechanism design and is advantageous, over the traditional RCM adjustments. The potential of the proposed concept lies in the fact that it is not limited to the adopted mechanism only rather, it is extendable to other mechanisms as well which was missing in the prior art. In addition, it offers a more simplified and economical design. Further, an initial prototype of the exemplary mechanism is manufactured using laser micromachining and rapid prototyping techniques. Both analytical as well as physical validations have been performed which proved the efficacy of the proposed kinematic modeling concept. Furthermore, the prototype of the introduced RCM Adjustment System is manufactured as a single-piece design which demonstrated its effectiveness. The preferred embodiment of the same is the said double-parallelogram-based RCM mechanism which is most widely used for robot-assisted minimally invasive surgery (RAMIS). It is designed in a SolidWorks environment and manufactured using Al6101-T6 and SS304 as link material, and a variety of standard elements.
Export Participation and Employment of Contract Workers in Organized Manufacturing Plants in India
Authors: Goldar Bishwanath
Published: July 2023

The share of contract workers, i.e., the workers employed through contractors, as against the workers directly employed by factories, out of all workers employed in India’s organized manufacturing has increased significantly over the last three decades, reaching 38.6% in 2019–2020. Using plant-level panel data for the years 2008–2009 to 2017–2018, the paper examines econometrically whether export participation by the organized sector manufacturing plants in India induced them to hire contract workers. The empirical results indicate that the export market participation by manufacturing plants tends to raise their probability of employing contract workers. This effect of export participation on the probability of employing contract workers is found for plants in the medium-low and low-technology industries, and the impact is bigger in the medium-high and high-technology industries. The effect is found to be relatively stronger for bigger plants than small-sized plants. In high-technology industries, in-house firm-specific skill formation is important for firms for their being able to export their products, and, hence, in many technology-oriented plants of these industries export participation should not induce them to employ contract workers. Some support for this hypothesis is found in the empirical results obtained in the study.
Evaluation of the Response Rates of Hospitals in the Prevention and Control of COVID-19 in Hamadan Province of Iran
Authors: Seif Rabiei Mohammad Ali , Keramat Fariba , Sedighi Iraj , Jalili Ebrahim , Nikooseresht Mahshid , Talebi Seyed Saman , Kharghani Moghadam Seyedeh Melika
Published: July 2023

The COVID-19 pandemic has challenged the capacity of health systems in various countries. This study was designed to evaluate the response rates of hospitals in the prevention and control of COVID-19 in Hamadan province, Iran. In this cross-sectional study, all 20 hospitals in Hamadan province were monitored in February 2020. The instrument used in this study was the WHO checklist, entitled “Infection prevention and control health-care facility response for COVID-19.” In each hospital, the hospital infection control officials completed the checklists under the supervision of the hospital managers and were then provided to the project manager. Data analyzed by SPSS 22 software. There were 3482 hospital beds (the average beds of each hospital was 174) in the studied hospitals. Of 15 055 patients admitted to all hospitals, 2196 (14.6%) individuals were COVID-19 patients. The total average score obtained from the checklist was 62.25. Among 7 domains studied, the lowest scores belonged to 2 domains of “infrastructure and equipment” and “patient screening and triage.” The scores of domains “IPC programs” and “visitors” were significantly higher in hospitals with ICU beds than other hospitals ( P-value = .03 in both domains). A comparison between university-teaching hospitals with other hospitals and those in Hamadan city with other cities revealed no significant differences in any of the domains. The mean response rate (62.25) of hospitals in terms of COVID-19 in Hamadan province indicates their relative readiness to prevent and control the COVID19 pandemic. The shortage of infrastructure and equipment and screening and triage problems of patients were the main challenges of hospitals in managing the COVID-19 pandemic in Hamadan province, Iran.
Australia: No party convergence where we would most expect it
Authors: Mussel Johanan D. , Schlechta Henry
Published: July 2023

Classic spatial theory expects parties to converge on the same ideological position given certain assumptions. Many of these assumptions fail to hold in most countries, which may account for why this prediction frequently fails to materialise. Due to a unique combination of institutions, Australia presents the best chance for the theory to work: all votes must flow to either of the two major parties, parties approximate unitary actors, and elections see turnout as high as any democracy. If convergence should happen anywhere, it should be in Australia, and many argue that Australian parties indeed fulfil this prediction. However, in contrast to much of the literature, we do not find Australian politics to be unusually centrist. Based on five measures of ideology, we do not find convincing evidence that Australia’s party system is any more convergent than any other.
Cocaine and the port: Utopias of security, urban relations, and displacement of policing efforts in the port of Piraeus
Authors: Sergi Anna
Published: July 2023

In large commercial seaports policing and security efforts to counter the drug trade, especially cocaine, do not appear to be effective beyond a mere displacement effect. In the port of Piraeus, Greece, (perceived) rising quantities of cocaine have led to calls for further securitisation of the port to curb illicit trafficking. This article will present the current trends of countering and disrupting cocaine at the port of Piraeus and question how these efforts, together with the growth of the port, are affecting the overall territory of and around the port. This article will first argue that the (perceived) increase in cocaine trade towards/in the port of Piraeus has activated a ‘utopia of security’ in the policing and security responses at the port. This utopia of security leads to paradoxes when it comes to being effective against organised crime in the port. The article will conclude by discussing the possibility of a different approach, one of displacement of countering efforts rather than of cocaine flows. This different approach can also rebalance the focus of policing and security authorities on the relationship between the port and its territory.
Incidence and Prognostic Associations of Early Postoperative Stroke and Death Among Patients Undergoing Inner Branched Thoracic Endovascular Repair of Aortic Arch Pathologies: A Systematic Review and Meta-Analysis
Authors: Gao Jiang-Ping , Guo Wei , Zhang Hong-Peng
Published: July 2023

Objective:

The objective of this systematic review was to assess the incidences and associations of early postoperative stroke and death among patients undergoing inner branched thoracic endovascular aortic repair (TEVAR) of arch pathologies.

Methods:

Electronic bibliographic sources (PUBMED, EMBASE, and CENTRAL) were searched up to February 2022 using a combination of thesaurus and free-text terms to identify the studies using branched TEVAR to treat aortic arch disease. The systematic review was conducted according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines. All observational studies investigating the prognosis of inner branched TEVAR in the treatment of aortic arch pathologies were included. Independent extraction of articles was performed by two authors using predefined data fields, including study quality indications. All pooled analyses were based on a random-effects or fixed model according to the heterogeneity.

Results:

A total of 23 case series totaling 532 participants were included after screening. After optimized selection (largest sample size, most detailed data, lowest risk of bias) from the overlapping data, 12 studies with 289 participants were included in data synthesis. The pooled incidence of 30-day postoperative stroke was 10.6% (95% CI 7.0%–14.2%; p=.41, I2=3%). Pooled 30-day mortality was 4.9% (95% CI 2.0%–7.8%; p=.38, I2=7%). Combined early stroke/death was 15.7% (95% CI 11.2%–20.3%; p=.30, I2=15%). Subgroup analyses of 11 studies (without data missing) showed that a higher incidence of 30-day postoperative stroke was found in studies with aged participants (age≥71.3 years, p=.010), the higher percentage of COPD (≥30%, p= .011) and non–dissection-related pathologies (≥60.8%, p=.011). The higher 30-day postoperative mortality was found in studies with a high percentage of previous coronary artery disease (≥34.5%, p=.023).

Conclusions:

This review demonstrated that there were acceptable rates of 30-day postoperative stroke and death among patients undergoing inner branched TEVAR. It is strongly necessary to perform a rigorous risk assessment of aortic plaque embolism and coronary artery disease when the surgical plan of the inner branched TEVAR is determined.

Clinical Impact

Treatment arch pathologies with inner branched TEVAR provides acceptable early stroke rate and mortality. Aortic pathology mainly influenced the early stroke rate, and early recognizing high-risk patients for aortic plaque embolism is of supreme importance for reducing the early stroke rate. In addition, the history of coronary artery disease was strongly associated with early mortality, and attention should be paid to the coronary artery assessment and perioperative management of these patients.
Long-term Care of Living Kidney Donors Needs a Better Model of Healthcare Delivery
Authors: Loban Katya , Robert Jorane-Tiana , Alam Ahsan , Sandal Shaifali
Published: July 2023

Every year, over 30,000 healthy individuals globally donate a kidney to a patient with kidney failure. These living kidney donors are at higher risk of some medical complications post-donation when compared with matched controls. Although the absolute risk of these complications is low, appropriate long-term care is essential to allow early detection and timely interventions. Some transplant centers follow living donors long-term, but many recommend that donors regularly see a primary care practitioner post-donation. However, primary care is currently not integrated with transplant centers, and the two often work in silos with little to no channels of communication with each other. As this model of care is suboptimal, existing evidence suggests that post-donation care and follow-up are inadequate. We argue for an integrated model of living donor care with stronger continuity and coordination between primary care and transplant centers that are developed with the input of all relevant stakeholders.
Public communication of technoscience in the news: A cross-linguistic Multidimensional analysis of English and Italian newspapers
Authors: Zorzi Virginia , Neresini Federico , Cammozzo Alberto
Published: July 2023

Technoscience has historically been endowed by modern societies with cultural authority, which has undergone significant questioning since the end of WWII. In this study, we consider technoscience communication in online newspapers to test whether its linguistic and communicative features differentiate it from other types of news, possibly reflecting greater cultural authority. Focusing on news in English versus Italian, we apply Multidimensional analysis to a comparable English-Italian online news corpus comparing technoscience-related articles with all remaining articles. Results indicate overall linguistic and communicative homogeneity between science and other domains. Nevertheless, small differences seem to suggest an effort to make technoscience accessible and engaging in English, whereas Italian technoscience-related news tends to be more formal and richer in specialised information.
Abdominal Calcifications in a Patient From the Congo Basin with History of Snake Meat Consumption: Radiographic Evidence of Prior Armillifer Infection
Authors: Ramachandran Rajarajeshwari , Kumar Vikash , Isaac-Coss Giovannie , Huang Tiangui , Venugopal Sushma
Published: July 2023

We report a case of a 60-year-old asymptomatic male with history of consumption of uncooked snake meat while living in the Congo basin and prior imaging showing multiple abdominal calcifications. Patient had multiple subepithelial colonic lesions identified during screening colonoscopy and microscopic examination of the lesions demonstrated a calcified nodule in the submucosa with overlying normal mucosa. However, no parasite was identified within the calcified nodule. Given the history of consumption of uncooked snake meat and the typical radiographic feature of multiple abdominal calcifications, it is very likely that the patient’s radiographic abnormalities are due to prior Armillifer armillatus infection, a parasitic infection acquired from consumption of uncooked snake meat. Patient was asymptomatic at the time of evaluation and was not given anti-parasitic treatment.
Case Presentation: Re-revision Quad Tendon Repair and Vastus Advancement
Authors: Vadhera Amar S. , Gursoy Safa , Sivasundaram Lakshman , Lee Jonathan S. , Singh Harsh , Bunachita Sean , Chahla Jorge
Published: July 2023

Background:

The quadriceps tendon is crucial in allowing knee extension, attaching distally to the patella. While ruptures of the tendon are rare, retear can lead to muscle atrophy and complex tendon deficits with retraction which may make revision repairs difficult to perform. To avoid re-rupture, the repair is commonly augmented with an allograft or autograft, theoretically strengthening the repair.

Indications:

Patients are indicated for surgery when presenting with a chronic, symptomatic quadriceps rupture verified on provocative testing and advanced imaging.

Technique Description:

Adhesions and fibrotic tissues around the vastus muscles and tendon are released. Sutures and anchor materials from the previous reconstruction are removed. Fibrotic tissues at the distal end of the retracted quad tendon are removed. The tendon is then loosened from the proximal aspect with the applied traction through the suspension suture placed distal to the tendon. The gap is measured and a V-Y quadricepsplasty was performed at twice the length of the measured gap. In the proximal aspect of the tendon, the limbs of the V-plasty are left incomplete. Next, traction is applied to allow for a tightening exertion to the patella. The proximal Y limb is sutured together in a side-to-side fashion to allow for tendon distalization while retaining the distal traction of the patellar tendon. The medial and lateral limbs were then closed with a suture, completing the tendon advancement. Fibrotic tissues around the superior pole of the patella are removed to prepare for tendon reattachment. Two anchors on the medial and lateral sides are placed, and the suture threads from both anchors are tied in a Krackow configuration. The medial and lateral-sided suture knots are then tied together over the tendon and covered again with the Achilles allograft, completing the repair.

Results:

Outcomes of revision quadriceps repair have been promising, with good to excellent functional outcomes, successful return to activities, and objective outcomes.

Discussion/Conclusion:

Although initial studies report favorable outcomes following current repair techniques, there is a lack of quality literature on outcomes following primary or revision quadriceps repair. Future studies are necessary to determine the reliability, efficacy, and clinical outcomes following this procedure.

Patient Consent Disclosure Statement:

The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.
Challenges and opportunity in mobility among older adults – key determinant identification
Authors: Maresova Petra , Krejcar Ondrej , Maskuriy Raihan , Bakar Nor Azlina Abu , Selamat Ali , Truhlarova Zuzana , Horak Jiri , Joukl Miroslav , Vítkova Lucie
Published: July 2023

Background

Attention is focused on the health and physical fitness of older adults due to their increasing age. Maintaining physical abilities, including safe walking and movement, significantly contributes to the perception of health in old age. One of the early signs of declining fitness in older adults is limited mobility. Approximately one third of 70-year-olds and most 80-year-olds report restrictions on mobility in their apartments and immediate surroundings. Restriction or loss of mobility is a complex multifactorial process, which makes older adults prone to falls, injuries, and hospitalizations and worsens their quality of life while increasing overall mortality.

Objective

The objective of the study is to identify the factors that have had a significant impact on mobility in recent years and currently, and to identify gaps in our understanding of these factors. The study aims to highlight areas where further research is needed and where new and effective solutions are required.

Methods

The PRISMA methodology was used to conduct a scoping review in the Scopus and Web of Science databases. Papers published from 2007 to 2021 were searched in November 2021. Of these, 52 papers were selected from the initial 788 outputs for the final analysis.

Results

The final selected papers were analyzed, and the key determinants were found to be environmental, physical, cognitive, and psychosocial, which confirms the findings of previous studies. One new determinant is technological. New and effective solutions lie in understanding the interactions between different determinants of mobility, addressing environmental factors, and exploring opportunities in the context of emerging technologies, such as the integration of smart home technologies, design of accessible and age-friendly public spaces, development of policies and regulations, and exploration of innovative financing models to support the integration of assistive technologies into the lives of seniors.

Conclusion

For an effective and comprehensive solution to support senior mobility, the determinants cannot be solved separately. Physical, cognitive, psychosocial, and technological determinants can often be perceived as the cause/motivation for mobility. Further research on these determinants can help to arrive at solutions for environmental determinants, which, in turn, will help improve mobility. Future studies should investigate financial aspects, especially since many technological solutions are expensive and not commonly available, which limits their use.
Enhancement of PD-L1-attenuated CAR-T cell function through breast cancer-associated fibroblasts-derived IL-6 signaling via STAT3/AKT pathways
Authors: Chuangchot Nisa , Jamjuntra Pranisa , Yangngam Supaporn , Luangwattananun Piriya , Thongchot Suyanee , Junking Mutita , Thuwajit Peti , Yenchitsomanus Pa-Thai , Thuwajit Chanitra
Published: July 2023

Background

Carcinoma-associated fibroblasts (CAFs) play a critical role in cancer progression and immune cell modulation. In this study, it was aimed to evaluate the roles of CAFs-derived IL-6 in doxorubicin (Dox) resistance and PD-L1-mediated chimeric antigenic receptor (CAR)-T cell resistance in breast cancer (BCA).

Methods

CAF conditioned-media (CM) were collected, and the IL-6 level was measured by ELISA. CAF-CM were treated in MDA-MB-231 and HCC70 TNBC cell lines and siIL-6 receptor (IL-6R) knocked down (KD) cells to determine the effect of CAF-derived IL-6 on Dox resistance by flow cytometry and on increased PD-L1 through STAT3, AKT and ERK1/2 pathways by Western blot analysis. After pre-treating with CM, the folate receptor alpha (FRα)-CAR T cell cytotoxicity was evaluated in 2D and 3D spheroid culture assays.

Results

The results showed a significant level of IL-6 in CAF-CM compared to that of normal fibroblasts (NFs). The CM with high IL-6 level significantly induced Dox resistance; and PD-L1 expression through STAT3 and AKT pathways in MDA-MB-231 and HCC70 cells. These induction effects were attenuated in siIL-6R KD cells. Moreover, the TNBC cell lines that were CM-treated with STAT3 and an AKT inhibitor had a reduced effect of IL-6 on PD-L1 expression. BCA cells with high IL-6 containing-CM treatment had resistance to cancer cell killing by FRα CAR-T cells compared to untreated cells.

Conclusion

These results highlight CAF-derived IL-6 in the resistance of chemotherapy and T cell therapy. Using inhibitors of IL6-STAT3/AKT-PD-L1 axis may provide a potential benefit of Dox and CAR-T cell therapies in BCA patients.
Pancreatic CT perfusion: quantitative meta-analysis of disease discrimination, protocol development, and effect of CT parameters
Authors: Skornitzke Stephan , Vats Neha , Mayer Philipp , Kauczor Hans-Ulrich , Stiller Wolfram
Published: July 2023

Background

This study provides a quantitative meta-analysis of pancreatic CT perfusion studies, investigating choice of study parameters, ability for quantitative discrimination of pancreatic diseases, and influence of acquisition and reconstruction parameters on reported results.

Methods

Based on a PubMed search with key terms ‘pancreas’ or ‘pancreatic,’ ‘dynamic’ or ‘perfusion,’ and ‘computed tomography’ or ‘CT,’ 491 articles published between 1982 and 2020 were screened for inclusion in the study. Inclusion criteria were: reported original data, human subjects, five or more datasets, measurements of pancreas or pancreatic pathologies, and reported quantitative perfusion parameters. Study parameters and reported quantitative measurements were extracted, and heterogeneity of study parameters and trends over time are analyzed. Pooled data were tested with weighted ANOVA and ANCOVA models for differences in perfusion results between normal pancreas, pancreatitis, PDAC (pancreatic ductal adenocarcinoma), and non-PDAC (e.g., neuroendocrine tumors, insulinomas) and based on study parameters.

Results

Reported acquisition parameters were heterogeneous, except for contrast agent amount and injection rate. Tube potential and slice thickness decreased, whereas tube current time product and scan coverage increased over time. Blood flow and blood volume showed significant differences between pathologies (both p < 0.001), unlike permeability (p = 0.11). Study parameters showed a significant effect on reported quantitative measurements (p < 0.05).

Conclusions

Significant differences in perfusion measurements between pathologies could be shown for pooled data despite observed heterogeneity in study parameters. Statistical analysis indicates most influential parameters for future optimization and standardization of acquisition protocols.

Critical relevance statement

Quantitative CT perfusion enables differentiation of pancreatic pathologies despite the heterogeneity of study parameters in current clinical practice.

Graphical abstract
Radiomic signatures from T2W and DWI MRI are predictive of tumour hypoxia in colorectal liver metastases
Authors: Bodalal Zuhir , Bogveradze Nino , ter Beek Leon C. , van den Berg Jose G. , Sanders Joyce , Hofland Ingrid , Trebeschi Stefano , Groot Lipman Kevin B. W. , Storck Koen , Hong Eun Kyoung , Lebedyeva Natalya , Maas Monique , Beets-Tan Regina G. H. , Gomez Fernando M. , Kurilova Ieva
Published: July 2023

Background

Tumour hypoxia is a negative predictive and prognostic biomarker in colorectal cancer typically assessed by invasive sampling methods, which suffer from many shortcomings. This retrospective proof-of-principle study explores the potential of MRI-derived imaging markers in predicting tumour hypoxia non-invasively in patients with colorectal liver metastases (CLM).

Methods

A single-centre cohort of 146 CLMs from 112 patients were segmented on preoperative T2-weighted (T2W) images and diffusion-weighted imaging (DWI). HIF-1 alpha immunohistochemical staining index (high/low) was used as a reference standard. Radiomic features were extracted, and machine learning approaches were implemented to predict the degree of histopathological tumour hypoxia.

Results

Radiomic signatures from DWI b200 (AUC = 0.79, 95% CI 0.61–0.93, p = 0.002) and ADC (AUC = 0.72, 95% CI 0.50–0.90, p = 0.019) were significantly predictive of tumour hypoxia. Morphological T2W TE75 (AUC = 0.64, 95% CI 0.42–0.82, p = 0.092) and functional DWI b0 (AUC = 0.66, 95% CI 0.46–0.84, p = 0.069) and b800 (AUC = 0.64, 95% CI 0.44–0.82, p = 0.071) images also provided predictive information. T2W TE300 (AUC = 0.57, 95% CI 0.33–0.78, p = 0.312) and b = 10 (AUC = 0.53, 95% CI 0.33–0.74, p = 0.415) images were not predictive of tumour hypoxia.

Conclusions

T2W and DWI sequences encode information predictive of tumour hypoxia. Prospective multicentre studies could help develop and validate robust non-invasive hypoxia-detection algorithms.

Critical relevance statement

Hypoxia is a negative prognostic biomarker in colorectal cancer. Hypoxia is usually assessed by invasive sampling methods. This proof-of-principle retrospective study explores the role of AI-based MRI-derived imaging biomarkers in non-invasively predicting tumour hypoxia in patients with colorectal liver metastases (CLM).

Graphical Abstract
Copper chelation suppresses epithelial-mesenchymal transition by inhibition of canonical and non-canonical TGF-β signaling pathways in cancer
Authors: Poursani Ensieh M. , Mercatelli Daniele , Raninga Prahlad , Bell Jessica L. , Saletta Federica , Kohane Felix V. , Neumann Daniel P. , Zheng Ye , Rouaen Jourdin R. C. , Jue Toni Rose , Michniewicz Filip T. , Schadel Piper , Kasiou Erin , Tsoli Maria , Cirillo Giuseppe , Waters Shafagh , Shai-Hee Tyler , Cazzoli Riccardo , Brettle Merryn , Slapetova Iveta , Kasherman Maria , Whan Renee , Souza-Fonseca-Guimaraes Fernando , Vahdat Linda , Ziegler David , Lock John G. , Giorgi Federico M. , Khanna KumKum , Vittorio Orazio
Published: July 2023

Background

Metastatic cancer cells exploit Epithelial-mesenchymal-transition (EMT) to enhance their migration, invasion, and resistance to treatments. Recent studies highlight that elevated levels of copper are implicated in cancer progression and metastasis. Clinical trials using copper chelators are associated with improved patient survival; however, the molecular mechanisms by which copper depletion inhibits tumor progression and metastasis are poorly understood. This remains a major hurdle to the clinical translation of copper chelators. Here, we propose that copper chelation inhibits metastasis by reducing TGF-β levels and EMT signaling. Given that many drugs targeting TGF-β have failed in clinical trials, partly because of severe side effects arising in patients, we hypothesized that copper chelation therapy might be a less toxic alternative to target the TGF-β/EMT axis.

Results

Our cytokine array and RNA-seq data suggested a link between copper homeostasis, TGF-β and EMT process. To validate this hypothesis, we performed single-cell imaging, protein assays, and in vivo studies. Here, we used the copper chelating agent TEPA to block copper trafficking. Our in vivo study showed a reduction of TGF-β levels and metastasis to the lung in the TNBC mouse model. Mechanistically, TEPA significantly downregulated canonical (TGF-β/SMAD2&3) and non-canonical (TGF-β/PI3K/AKT, TGF-β/RAS/RAF/MEK/ERK, and TGF-β/WNT/β-catenin) TGF-β signaling pathways. Additionally, EMT markers of MMP-9, MMP-14, Vimentin, β-catenin, ZEB1, and p-SMAD2 were downregulated, and EMT transcription factors of SNAI1, ZEB1, and p-SMAD2 accumulated in the cytoplasm after treatment.

Conclusions

Our study suggests that copper chelation therapy represents a potentially effective therapeutic approach for targeting TGF-β and inhibiting EMT in a diverse range of cancers.
Tobacco smoke incursion into private residences in Israel: a cross-sectional study examining public perceptions of private rights and support for governmental policies
Authors: Theitler Noa , Rees Vaughan W. , Peled-Raz Maya , Bitan Michal , Rosen Laura J.
Published: July 2023

Background

Tobacco smoke incursion (TSI) into private residences is a widespread problem in many countries. We sought to assess the prevalence of self-reported TSI and public attitudes about TSI in Israel, a country with a relatively high smoking prevalence and high population density.

Methods

We conducted a random digit dial survey among residents in Israel (N = 285) in 2017, which examined the frequency, source, correlates of, and attitudes towards TSI and potential regulatory options. The cooperation rate was 63.9%.

Results

Among respondents, 44.7% reported ever experiencing home TSI, with higher exposure among residents of multi-unit housing (MUH) (MUH versus private homes: aOR (Adjusted Odds Ratio): 3.60, CI (Confidence Interval): [1.96, 6.58], p < .001). Most respondents (69.8%), including nearly half of smokers, prioritized the right of individuals to breath smoke-free air in their apartments over the right of smokers to smoke in their apartments. Women and non-smokers were more likely to support the right to breathe smoke-free air (Women versus men: aOR: 2.77 CI: [1.48, 5.16], p = .001; Nonsmokers versus smokers: aOR: 3.21 CI [1.59, 6.48], p = .001). However, only about a quarter (24.8%) of respondents who ever experienced TSI raised the issue with the neighbor who smoked, the neighbor's landlord, or the building committee. The vast majority (85.2%) of all respondents, including three-quarters of smokers, supported smoke-free legislation for multi-unit housing (MUH), with those ever-exposed to TSI and non-smokers more likely to support legislation (ever-exposed versus never-exposed aOR = 2.99, CI [1.28, 6.97], p = 0.011; nonsmokers versus smokers aOR = 3.00, CI [1.28, 7.01], p = 0.011).

Conclusions

Among study participants, tobacco smoke incursion was a common, yet unwelcome experience. Most respondents believed that the right to breathe smoke-free air in one's apartment superseded that of neighbors to smoke anywhere in their home, and most supported legislation to prevent TSI. Though further study is needed to understand better TSI and effective methods for its prevention, our findings suggest that policy interventions, including legal action at the level of the Supreme Court and/or the Knesset, are needed. Regulation, policy initiatives and campaigns to denormalize smoking in proximity to other people and private residences globally could reduce the scope of this widespread problem, protect individuals from home TSI, and improve population health.
Long non-coding RNA CCHE1 modulates LDHA-mediated glycolysis and confers chemoresistance to melanoma cells
Authors: Ding Zhi , Yang Junyi , Wu Baojin , Wu Yingzhi , Guo Fanli
Published: July 2023

Melanoma is considered as the most common metastatic skin cancer with increasing incidence and high mortality globally. The vital roles of long non-coding RNAs (lncRNAs) in the tumorigenesis of melanoma are elucidated by emerging evidence. The lncRNA cervical carcinoma high-expressed 1 (CCHE1) was overexpressed and acted as an oncogene in a variety of cancers, while the function of CCHE1 in melanoma remains unclear. Here, we found that CCHE1 was highly expressed in melanoma and correlated with the poorer survival of melanoma patients. Depletion of CCHE1 inhibited the proliferation, induced cell apoptosis and suppressed in vivo tumor growth. To further understand the functional mechanism of CCHE1, the interacting partners of CCHE1 were identified via RNA pull-down assay followed by mass spectrometry. CCHE1 was found to bind lactate dehydrogenase A (LDHA) and acted as a scaffold to enhance the interaction of LDHA with the fibroblast growth factor receptor type 1 (FGFR1), which consequently enhanced LDHA phosphorylation and activity of LDHA. Inhibiting CCHE1 strikingly suppressed the glycolytic flux of melanoma cells and lactate generation in vivo. Further study demonstrated that CCHE1 desensitized melanoma cells to dacarbazine and inhibition of glycolysis reversed CCHE1-induced chemoresistance. These results uncovered the novel function of CCHE1 in melanoma by reprogramming the glucose metabolism via orchestrating the activity of LDHA.
Laparoscopic resection of a metastatic myxoid liposarcoma in the mesentery of the small intestine: a case report
Authors: Kamehama Fumika , Kinjo Tatsuya , Miyagi Yoshihiro , Furugen Tomonori , Teruya Takao , Tamaki Tomoko , Wada Naoki , Takatsuki Mitsuhisa
Published: July 2023

Background

Myxoid liposarcoma (MLS), with its risk factors, tends to spread to the lungs and extraperitoneally, with intraperitoneal metastases occurring rarely. We present an unusual case of a myxoid liposarcoma that metastasized to the abdominal organs.

Case presentation

A 60-year-old female patient was referred to our hospital for the evaluation of a right upper limb tumor that had been growing for 7 years. The patient refused surgery, and during follow-up, tumor hemorrhage resulted in hemorrhagic shock. The patient’s right upper limb was immediately amputated. MLS was diagnosed histopathologically. Subsequently, the patient underwent adjuvant chemotherapy. Computed tomography (CT) revealed a right buttock mass, a pelvic mass, and left cardiophrenic angle lymph nodes 3 years after the initial surgery. Contrast-enhanced abdominal CT revealed a relatively low-density, lobulated pelvic tumor. Contrast-enhanced pelvic magnetic resonance imaging (MRI) revealed a low-intensity, lobulated mass on T1-weighted images and a high-intensity mass on T2-weighted images. The pelvic mass showed no significant fluorodeoxyglucose (FDG) uptake on positron emission tomography (PET)-CT. On clinical examination, gynecological malignancies were ruled out as the origin of the pelvic lesions. After resection of the right buttock mass, pelvic mass, and left cardiophrenic angle lymph nodes, the patient underwent laparoscopic surgery for a preoperative diagnosis of small intestinal mesenteric metastasis of MLS. A tumor was found in the mesentery of the small intestine and removed with a margin of 5 cm on both the proximal and distal sides. The specimen measured 10 × 8 × 5 cm and contained a multifocal mass. The tumor was found in the mesentery of the small intestine, with no mucosal or submucosal invasion. The patient was diagnosed with MLS with small mesenteric intestinal metastases. On postoperative day 8, the patient was discharged after an uneventful postoperative course. Twelve months after the surgery, there was no evidence of local or distant recurrence.

Conclusions

Small intestinal mesenteric metastases of MLSs are rare. Moreover, there are few reports on laparoscopic resection. In this case, the laparoscopic approach was useful in detecting the tumor location and determining the range of resection.
Giant sacral schwannoma in a neurofibromatosis type 2 patient
Authors: Seth Namdev , Varshney Dushyant , Verma Saumya
Published: July 2023

Background

Neurofibromatosis type 2 is an autosomal dominant disorder, mainly characterized by multiple neurological lesions, such as schwannomas, meningiomas, neurofibromas and intramedullary ependymomas. Schwannomas are usually small circumscribed lesion. Sacral location of a schwannoma with cystic change is a very rare finding. We are presenting one such case with giant cystic schwannoma with fluid–fluid levels in sacral region.

Case presentation

We present a case of 13-year-old female patient, presenting with pelvic pain and gradually progressive bilateral lower limb weakness. On MRI, giant cystic schwannoma with internal fluid–fluid levels was noted in sacral region, extending anteriorly into the presacral region, causing mass effects on pelvic organs, which explained the cause of her symptoms. She also showed the presence of bilateral vestibular schwannoma and multiple small cerebral lesions, leading to the diagnosis of neurofibromatosis type 2.

Conclusions

Our current case of neurofibromatosis type 2, diagnosed by presence of bilateral vestibular schwannoma, shows atypically large sacral cystic schwannomas and cerebral subcortical lesions, probably representing glial microhamartomas. Sacral schwannomas can be of giant size with cystic changes and fluid–fluid levels, mimicking aneurismal bone cyst, as in current case.
Effect of alternative load path design method on preventing progressive collapse and reducing the rehabilitation cost using FEMA-P58
Authors: Ashoub Mahmoud Mohamed Nabil Maher , Attia Walid Abdel Latif
Published: July 2023

The alternative load path (ALP) method is an important modern design approach to prevent progressive collapse of buildings by redesigning adjacent structural elements to provide an alternative load path in case of partial collapse. This may lead to tangible changes in the mass and stiffness of the building, which means a change in the seismic behavior of the building. This shows the fact that choosing and designing an alternative load path is not an easy task. Studying the improvement in the seismic behavior of the building, whether direct or indirect, as a result of using the ALP, is the main objective of this paper. To achieve this goal, this study examined approximately 96 models, including the study of the possibility of progressive collapse occurring, whether there is or isn't an alternative path for the loads using non-linear static and dynamic analysis, as well as evaluating the study models using various seismic evaluation methods, where both the nonlinear static pushover analysis method and the FEMA-P58 method were used and compared. The study models are divided into two sections, with and without (ALP). The study models are also categorized by building height using heights of 6, 9, 12 and 15 floors. The models were also divided based on the locations of weak points in the original design that may be susceptible to damage and lead to progressive collapse, whether they are internal, side, or corner weak columns. The findings from the examined models indicate that the efficiency of (ALP) systems is more prominent in low and mid-height buildings compared to tall buildings. Additionally, the results demonstrate that the FEMA-P58 method yields similar predictions regarding building behavior as traditional seismic assessment methods like pushover analysis, but in a simplified manner that benefits non-engineers by providing clear insights into the financial and temporal aspects of different structural system solutions.
Assessing the potential of Other Effective area-based Conservation Measures (OECMs) for contributing to conservation targets: A global scoping review protocol
Authors: Petza Dimitra , Amorim Eva , Ben Lamine Emma , Colloca Francesco , Dominguez Crisóstomo Esther , Fabbrizzi Erika , Fraschetti Simonetta , Galparsoro Ibon , Giakoumi Sylvaine , Kruse Maren , Stelzenmüller Vanessa , Katsanevakis Stelios
Published: July 2023

This scoping review (ScR) protocol aims to establish the methodological approach for identifying and mapping the evidence regarding the actual contribution of Other Effective area-based Conservation Measures (OECMs) to spatial conservation targets. Emphasis will be placed on examining the research conducted, including the methodologies applied, and analyzing both good practices and acknowledged failures. OECMs, introduced by the Convention on Biological Diversity (CBD) in 2010, refer to areas outside of protected areas, such as fisheries restricted areas, archaeological sites, and military areas, that effectively conserve biodiversity in-situ over the long term. OECMs are recognized rather than designated. Many countries currently endeavor to identify, recognize and report OECMs to the CBD for formal acceptance to support the implementation of spatial conservation targets. Studies that assess the contribution of OECMs to spatial conservation targets will be considered. Potential OECMs with primary, secondary or ancillary conservation objectives established by all sectors in the terrestrial, freshwater and marine realm worldwide will be considered. Peer-reviewed and grey literature will be considered without imposing limitations based on publication year, stage, subject area and source type. Both experimental and observational studies in English, French, Greek, Italian, and Spanish will be reviewed. The ScR will follow the Joanna Briggs Institute (JBI) methodology. The protocol will be guided by the PRISMA-ScR (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) extension for scoping reviews. The search will encompass bibliographic databases such as Scopus, Web of Science and Google Scholar. Grey literature sources will include databases, pre-print archives and organizational websites. The Covidence platform will be utilized for data management and extraction.