bluuebunny/crossref_metadata_2025 huggingface dataset:
Bone Marrow Mesenchymal Stromal Cells (BMMSCs) Augment Osteointegration of Dental Implants in Type 1 Diabetic Rabbits: An X-Ray Micro-Computed Tomographic Evaluation
Authors: Abdullah Alqahtani Nabeeh , C. Chandramoorthy Harish , Shaik Sharaz , Syed Jamaluddin , Chowdhary Ramesh , Antony Leoney
Published: March 2020

Background and objectives: The study aimed to investigate the effect of bone marrow mesenchymal stromal cells (BMMSCs) on implant-bone osseointegration in type I diabetic New Zealand rabbits. Materials and methods: BMMSCs harvested from healthy rabbits were processed and validated for purity and osteocyte differentiability. Mandibular incisors of diabetic and control rabbits were carefully extracted, and the sockets were plugged with collagen sponges. Platelet-rich plasma (PRP) containing osteoinductive BMMSCs, and plain PRP were injected into the collagen sponge of the right and left sockets respectively. Dental implants of 2.6 mm diameter and 10 mm length were inserted into the collagen sponge of both sockets. All the animals were sacrificed six weeks post surgery to evaluate an early stage of osseointegration; the mandibles scanned by X-ray microcomputed tomography (μCT) and subjected to 3D analysis. The μCT parameters of the right implant were paired against that of the left side of each animal and analyzed by paired T-test. Results: The preclinical evaluation of the viability and osteocyte differentiation of the BMMSCs were consistent between both the donor samples. The osseointegration of dental implants with stem cell therapy (BMMSCs + PRP + collagen) in normal and diabetic rabbits was significantly higher than that of implants with adjunctive PRP + collagen only (p < 0.05). Conclusion: Stem Cell therapy with osteoinductive BMMSCs and PRP can offer a novel approach to enhance the osseointegration of dental implants in uncontrolled diabetic patients.
The Effects of Homogenizing and Quenching and Tempering Treatments on Crack Healing
Authors: Qiu Yao , Xin Ruishan , Luo Jianbin , Ma Qingxian
Published: March 2020

After thermal deformation and heat treatment, it can be observed that there are small, newly formed grains in the crack healing zone, which indicates that the internal crack is fully healed. However, in our previous study, the impact properties of the internal healing zone could only be partially healed. In this study, the process of homogenizing treatment was adopted to achieve grain size homogeneity. The effects of homogenizing treatment and quenching and tempering treatment on crack healing were also systematically analyzed. With the same heat treatment method, SA508-3 samples, which were subjected to multi-pass deformation, had a higher percentage recovery than those that underwent uniaxial compression. The percentage recovery of the crack healing zone was significantly improved after the process of homogenizing treatment. The impact property of the crack healing zone could be fully restored after homogenizing treatment followed by quenching and tempering treatment. However, after several episodes of heating, the grain-boundary strength decreased, and the impact value was relatively low.
Measurement of Surface Velocity in a 150 mm × 1270 mm Slab Continuous-Casting Mold
Authors: Wang Yang , Feng Jie , Yang Shufeng , Li Jingshe
Published: March 2020

Surface velocity in the continuous-casting mold needs to be studied to better control the quality of steel products. In this paper, the measurement of surface velocity in a 150 mm × 1270 mm slab continuous-casting mold was investigated. Taking the slag layer into consideration, a numerical simulation was performed which was validated by a particle image velocimetry test. A nail-board experiment was also conducted to measure surface velocity in the continuous-casting mold. The effect of nail diameter used in nail-board experiment on the measurement of the surface velocity was also discussed to improve the precision of nail-board experiment result. The results showed that the maximum surface velocity was 0.739 m/s around the mid-section of the free surface, and the results of nail-board experiments were more accurate when the steel nail diameter was 10 mm.
Are Large Particles of Iron Detrimental to Properties of Powder Metallurgy Steels?
Authors: Abdallah Ahmed , Habibnejad-Korayem Mahdi , Malakhov Dmitri V.
Published: March 2020

It is experimentally shown that a removal of particles exceeding 100 microns in size from iron powders typically used in the fabrication of medium density powder metallurgy steels has a weak effect on apparent density, flowability and compressibility of blends as well as on density and strength of green bodies. An elimination of such particles, i.e., cutting off a heavy tail of a size distribution histogram at the 100 μm threshold, improves a compositional uniformity of sintered materials, but has no noticeable beneficial effect upon the strength of a final product, which is likely be determined by a fraction of pores and their shapes. A presence of soft pearlitic inclusions hardly matters unless their number density becomes so large that a 3D continuity (integrity) of a hard martensitic matrix is lost. This finding suggests that such an expensive preparatory step as sieving away large particles from as-received mixtures would bear no technological advantages. It was experimentally found that an attempt to lower the threshold below 100 μm noticeably worsened apparent density, flowability and compressibility.
Development of Pressure-Responsive PolyPropylene and Biochar-Based Materials
Authors: Noori Amir , Bartoli Mattia , Frache Alberto , Piatti Erik , Giorcelli Mauro , Tagliaferro Alberto
Published: March 2020

In this research paper, we reported the synthesis of biochar-based composites using biochar derived from exhausted tea leaves and polypropylene. The resulting materials were deeply characterized investigating mechanical (dynamic mechanical thermal analysis), thermal (thermogravimetrical analysis and differential scanning calorimetry), morphological (field emission scanning microscopy) and electrical properties vs. temperature. Furthermore, electrical conductivity was studied for a wide range of pressures showing an irreversible plastic deformation. An increment of one order of magnitude in the conductivity was observed in the case of 40 wt% biochar loading, reaching a value of 0.2 S/m. The material produced exhibited the properties of an irreversible pressure sensor.
Dielectric Characterization and Separation Optimization of Infiltrating Ductal Adenocarcinoma via Insulator-Dielectrophoresis
Authors: Adekanmbi Ezekiel O. , Giduthuri Anthony T. , Srivastava Soumya K.
Published: March 2020

The dielectrophoretic separation of infiltrating ductal adenocarcinoma cells (ADCs) from isolated peripheral blood mononuclear cells (PBMCs) in a ~1.4 mm long Y-shaped microfluidic channel with semi-circular insulating constrictions is numerically investigated. In this work, ADCs (breast cancer cells) and PBMCs’ electrophysiological properties were iteratively extracted through the fitting of a single-shell model with the frequency-conductivity data obtained from AC microwell experiments. In the numerical computation, the gradient of the electric field required to generate the necessary dielectrophoretic force within the constriction zone was provided through the application of electric potential across the whole fluidic channel. By adjusting the difference in potentials between the global inlet and outlet of the fluidic device, the minimum (effective) potential difference with the optimum particle transmission probability for ADCs was found. The radius of the semi-circular constrictions at which the effective potential difference was swept to obtain the optimum constriction size was also obtained. Independent particle discretization analysis was also conducted to underscore the accuracy of the numerical solution. The numerical results, which were obtained by the integration of fluid flow, electric current, and particle tracing module in COMSOL v5.3, reveal that PBMCs can be maximally separated from ADCs using a DC power source of 50 V. The article also discusses recirculation or wake formation behavior at high DC voltages (>100 V) even when sorting of cells are achieved. This result is the first step towards the production of a supplementary or confirmatory test device to detect early breast cancer non-invasively.
Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory
Authors: Morgunov Igor G. , Kamzolova Svetlana V. , Karpukhina Olga V. , Bokieva Svetlana B. , Lunina Julia N. , Inozemtsev Anatoly N.
Published: March 2020

Within this work, the microbial synthesis of (2R,3S)-isocitric acid (ICA), a metabolite of the nonconventional yeast Yarrowia lipolytica, from biodiesel waste, has been studied. The selected strain Y. lipolytica VKM Y-2373 synthesized ICA with citric acid (CA) as a byproduct. This process can be regulated by changing cultivation conditions. The maximal production of ICA with the minimal formation of the byproduct was provided by the use of a concentration of (NH4)2SO4 (6 g/L); the addition of biodiesel waste to cultivation medium in 20–60 g/L portions; maintaining the pH of the cultivation medium at 6, and degree of aeration between 25% and 60% of saturation. Itaconic acid at a concentration of 15 mM favorably influenced the production of ICA by the selected strain. The optimization of cultivation conditions allowed us to increase the concentration of ICA in the culture liquid from 58.32 to 90.2 g/L, the product yield (Y) by 40%, and the ICA/CA ratio from 1.1:1 to 3:1. Research on laboratory animals indicated that ICA counteracted the negative effect of ammonium molybdate (10−5 M) and lead diacetate (10−7 M) on the learning and spatial memory of rats, including those exposed to emotional stress.
Cysteine Residues in Helicobacter pylori Adhesin HopQ Are Required for CEACAM–HopQ Interaction and Subsequent CagA Translocation
Authors: Hamway Youssef , Taxauer Karin , Moonens Kristof , Neumeyer Victoria , Fischer Wolfgang , Schmitt Verena , Singer Bernhard B. , Remaut Han , Gerhard Markus , Mejías-Luque Raquel
Published: March 2020

Attachment to the host gastric mucosa is a key step in Helicobacter pylori infection. Recently, a novel adhesin, HopQ, was shown to bind distinct host CEACAM proteins—an interaction that was found to be essential for the translocation of CagA, a key virulence factor of H. pylori. The HopQ–CEACAM1 co-crystal structure revealed a binding mode dependent on loops in HopQ that are clasped by disulfide bonds. In this study, we investigated the importance of these cysteine residues for CEACAM1 engagement by H. pylori. We observed a loss of CEACAM1 binding and CagA translocation upon disruption of the disulfide bond in loop CL1 (connecting C103 to C132 in HopQ). Deletion of the Dsb-like oxidoreductase HP0231 did not affect cell surface expression of HopQ or alter the interaction of H. pylori with target cells. Although HP0231 deletion was previously described to impede CagA translocation, our results indicate that this occurs through a HopQ-independent mechanism. Together, our results open up new avenues to therapeutically target the HopQ–CEACAM1 interaction and reduce the burden of pathogenic H. pylori.
Tunable Dewatering Behavior of Montmorillonite Suspension by Adjusting Solution pH and Electrolyte Concentration
Authors: Dong Yingdi , Li Hongliang , Fan Yuping , Ma Xiaomin , Sun Dong , Wang Yanhong , Gao Zhiyong , Dong Xianshu
Published: March 2020

Montmorillonite is always a troublemaker for the dewatering in coal processing since its existence can decrease the rates of sedimentation and filtration of coal slurry. To eliminate the adverse effect of montmorillonite, adjusting the slurry pH and adding electrolytes are always the key methods. However, the underlying mechanism still needs to be further studied. The dewatering of Na-montmorillonite (Na-Mt) suspensions has been studied as a function of NaCl concentration (10−3, 10−2, and 10−1 M) at different pH values (6.0, 7.7, 8.1, 9.2). The point of zero charge of edge surface of Na-Mt (pHPZC,edge) appeared at the pH value of 6.8. The sedimentation and rheology experiments described the coagulation and flow behaviors of Na-Mt suspensions, respectively. The Na-Mt suspension coagulated spontaneously at low salt concentration with the pH ~ 6.0. For the pH > pHPZC,edge, the height of the sediment bed reduced and apparent viscosity increased with the increase of the electrolyte concentration. The filtration properties were evaluated on the basis of Darcy’s law. The obtained result clearly demonstrated that the filtration rate was accelerated with the increase of pH and electrolyte concentration. The modes of particle association and its effect on filtration performance were discussed. Moreover, a comparison with related results from the literature was performed. At pH ~ 6 and low electrolyte concentration, the positively charged Edge surfaces and negatively charged Face surfaces coagulate rapidly to form a sealed structure by electrostatic attraction. Furthermore, inside this sealed structure, the water molecules cannot be removed in the filtration process easily. However, by increasing the electrolyte concentration at pH > pHPZC,edge, the gradually formed Face/Face structure increases the filtration rate sharply because of the inhibiting effect of the electric double layer (EDL) and the osmotic expansion. Therefore, adjusting solution conditions of the aqueous suspension to tune the particle coagulation behavior is one of the effective methods to solve the problem of montmorillonite dewatering.
Heterogeneous Nucleation and Growth of CaCO3 on Calcite (104) and Aragonite (110) Surfaces: Implications for the Formation of Abiogenic Carbonate Cements in the Ocean
Authors: Tang Hongmei , Wu Xiao , Xian Haiyang , Zhu Jianxi , Wei Jingming , Liu Hongmei , He Hongping
Published: March 2020

Although near-surface seawater is supersaturated with CaCO3, only a minor part of it is abiogenic (e.g., carbonate cements). The possible reason for such a phenomenon has attracted much attention in the past decades. Substrate effects on the heterogeneous nucleation and growth of CaCO3 at various Mg2+/Ca2+ ratios may contribute to the understanding of the origin of abiogenic CaCO3 cements. Here, we used in situ atomic force microscopy (AFM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy to study the heterogeneous nucleation and growth of CaCO3 on both calcite (104) and aragonite (110) surfaces. The results show that (1) calcite spiral growth occurs on calcite (104) surfaces by monomer-by-monomer addition; (2) the aggregative growth of aragonite appears on aragonite (110) surfaces through a substrate-controlled oriented attachment (OA) along the [001] direction, followed by the formation of elongated columnar aragonite; and (3) Mg2+ inhibits the crystallization of both calcite and aragonite without impacting on crystallization pathways. These findings disclose that calcite and aragonite substrates determine the crystallization pathways, while the Mg2+/Ca2+ ratios control the growth rate of CaCO3, indicating that both types of CaCO3 substrate in shallow sediments and aqueous Mg2+/Ca2+ ratios constrain the deposition of abiogenic CaCO3 cements in the ocean.
U-Pb Zircon Dating of Migmatitic Paragneisses and Garnet Amphibolite from the High Pressure Seve Nappe Complex in Kittelfjäll, Swedish Caledonides
Authors: Bukała Michał , Majka Jarosław , Walczak Katarzyna , Włodek Adam , Schmitt Melanie , Zagórska Anna
Published: March 2020

The Seve Nappe Complex exposed in the Kittelfjäll area of the northern Scandinavian Caledonides comprises a volcano-sedimentary succession representing the Baltica passive margin, which was metamorphosed during the Iapetus Ocean closure. Garnet amphibolites, together with their host migmatitic paragneisses, record a potential (U)HP event followed by decompression-driven migmatization. The garnet amphibolites were originally thought to represent retrogressively altered granulites. The petrological and geochemical features of a studied garnet amphibolite allow for speculation about a peridotitic origin. Zirconium (Zr) content in rutile inclusions hosted in garnet in paragneisses points to near-peak temperatures between 738 °C and 780 °C, which is in agreement with the c. 774 °C obtained from the matrix rutile in the garnet amphibolite. The matrix rutile in multiple paragneiss samples records temperatures below 655 °C and 726 °C. Whereas the LA-ICP-MS U-Pb dating of zircon cores revealed the age spectrum from Paleoproterozoic to early Paleozoic, suggesting a detrital origin of zircon cores in paragneisses, the metamorphic zircon rims show an Early Ordovician cluster c. 475–469 Ma. Additionally, zircon cores and rims from the garnet amphibolite yielded an age of c. 473 Ma. The REE patterns of the Caledonian zircon rims from the paragneisses show overall low LREE concentrations, different from declining to rising trends in HREE (LuN/GdN = 0.49–38.76). Despite the textural differences, the cores and rims in zircon from the garnet amphibolite show similar REE patterns of low LREE and flat to rising HREE (LuN/GdN = 3.96–65.13). All zircon rims in both lithologies display a negative Eu anomaly. Hence, we interpret the reported ages as the growth of metamorphic zircon during migmatization, under granulite facies conditions related to exhumation from (U)HP conditions.
Ultrafine FeNi3 Nanocrystals Embedded in 3D Honeycomb-Like Carbon Matrix for High-Performance Microwave Absorption
Authors: Han Congai , Zhang Haiyan , Zhang Danfeng , Deng Yunfei , Shen Junyao , Zeng Guoxun
Published: March 2020

The reasonable design of magnetic carbon-based composites is of great significance to improving the microwave absorption (MA) performance of the absorber. In this work, ultrafine FeNi3 nanocrystals (5–7 nm) embedded in a 3D honeycomb-like carbon matrix (FeNi3@C) were synthesized via a facile strategy that included a drying and carbonization process. Because of the soft magnetic property of the FeNi3 nanocrystals and their unique 3D honeycomb-like structure, the FeNi3@C composites exhibit excellent MA abilities. When the filler loading ratio of FeNi3@C/paraffin composites is only 30 wt%, the maximum reflection loss (RL) value is −40.6 dB at 10.04 GHz. Meanwhile, an ultra-wide absorption frequency bandwidth of 13.0 GHz (5.0–18.0 GHz over −10 dB) can be obtained in the thickness range of 2.0–4.5 mm, and this means that the absorber can consume 90% of the incident waves. It benefits from the dual loss components, multiple polarizations, and multiple reflections for improving MA performances of FeNi3@C composites. These observations suggest that the 3D honeycomb-like FeNi3@C composites have broad application prospects in exploring new MA materials that have a wide frequency bandwidth and strong absorption.
In Situ Formation of Nanoporous Silicon on a Silicon Wafer via the Magnesiothermic Reduction Reaction (MRR) of Diatomaceous Earth
Authors: Aggrey Patrick , Abdusatorov Bakhodur , Kan Yuliya , Salimon Igor A. , Lipovskikh Svetlana A. , Luchkin Sergey , Zhigunov Denis M. , Salimon Alexey I. , Korsunsky Alexander M.
Published: March 2020

Successful direct route production of silicon nanostructures from diatomaceous earth (DE) on a single crystalline silicon wafer via the magnesiothermic reduction reaction is reported. The formed porous coating of 6 µm overall thickness contains silicon as the majority phase along with minor traces of Mg, as evident from SEM-EDS and the Focused Ion Beam (FIB) analysis. Raman peaks of silicon at 519 cm−1 and 925 cm−1 were found in both the film and wafer substrate, and significant intensity variation was observed, consistent with the SEM observation of the directly formed silicon nanoflake layer. Microstructural analysis of the flakes reveals the presence of pores and cavities partially retained from the precursor diatomite powder. A considerable reduction in surface reflectivity was observed for the silicon nanoflakes, from 45% for silicon wafer to below 15%. The results open possibilities for producing nanostructured silicon with a vast range of functionalities.
Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy
Authors: Huang Maojia , Liang Xinyue , Zhang Zixiao , Wang Jing , Fei Yiyan , Ma Jiong , Qu Songnan , Mi Lan
Published: March 2020

The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study. In this research, pH-sensitive carbon dots (CDs) were employed for intracellular pH sensing with fluorescence lifetime imaging microscopy (FLIM) for the first time. FLIM is a highly sensitive method that is used to detect a microenvironment and it can overcome the limitations of biosensing methods based on fluorescence intensity. The different groups on the CDs surfaces changing with pH environments led to different fluorescence lifetime values. The CDs aqueous solution had a gradual change from 1.6 ns to 3.7 ns in the fluorescence lifetime with a pH range of 2.6–8.6. Similar fluorescence lifetime changes were found in pH buffer-treated living cells. The detection of lysosomes, cytoplasm, and nuclei in living cells was achieved by measuring the fluorescence lifetime of CDs. In particular, a phasor FLIM analysis was used to improve the pH imaging. Moreover, the effects of the coenzymes, amino acids, and proteins on the fluorescence lifetime of CDs were examined in order to mimic the complex microenvironment inside the cells.
Nanomaterials for Periodontal Tissue Engineering: Chitosan-Based Scaffolds. A Systematic Review
Authors: Lauritano Dorina , Limongelli Luisa , Moreo Giulia , Favia Gianfranco , Carinci Francesco
Published: March 2020

Introduction. Several biomaterials are used in periodontal tissue engineering in order to obtain a three-dimensional scaffold, which could enhance the oral bone regeneration. These novel biomaterials, when placed in the affected area, activate a cascade of events, inducing regenerative cellular responses, and replacing the missing tissue. Natural and synthetic polymers can be used alone or in combination with other biomaterials, growth factors, and stem cells. Natural-based polymer chitosan is widely used in periodontal tissue engineering. It presents biodegradability, biocompatibility, and biological renewability properties. It is bacteriostatic and nontoxic and has hemostatic and mucoadhesive capacity. The aim of this systematic review is to obtain an updated overview of the utilization and effectiveness of chitosan-based scaffold (CS-bs) in the alveolar bone regeneration process. Materials and Methods. During database searching (using PubMed, Cochrane Library, and CINAHL), 72 items were found. The title, abstract, and full text of each study were carefully analyzed and only 22 articles were selected. Thirteen articles were excluded based on their title, five after reading the abstract, twenty-six after reading the full text, and six were not considered because of their publication date (prior to 2010). Quality assessment and data extraction were performed in the twelve included randomized controlled trials. Data concerning cell proliferation and viability (CPV), mineralization level (M), and alkaline phosphatase activity (ALPA) were recorded from each article Results. All the included trials tested CS-bs that were combined with other biomaterials (such as hydroxyapatite, alginate, polylactic-co-glycolic acid, polycaprolactone), growth factors (basic fibroblast growth factor, bone morphogenetic protein) and/or stem cells (periodontal ligament stem cells, human jaw bone marrow-derived mesenchymal stem cells). Values about the proliferation of cementoblasts (CB) and periodontal ligament cells (PDLCs), the activity of alkaline phosphatase, and the mineralization level determined by pure chitosan scaffolds resulted in lower than those caused by chitosan-based scaffolds combined with other molecules and biomaterials. Conclusions. A higher periodontal regenerative potential was recorded in the case of CS-based scaffolds combined with other polymeric biomaterials and bioceramics (bio compared to those provided by CS alone. Furthermore, literature demonstrated that the addition of growth factors and stem cells to CS-based scaffolds might improve the biological properties of chitosan.
Language of Health of Young Australian Adults: A Qualitative Exploration of Perceptions of Health, Wellbeing and Health Promotion via Online Conversations
Authors: Molenaar Annika , Choi Tammie S. T. , Brennan Linda , Reid Mike , Lim Megan S. C. , Truby Helen , McCaffrey Tracy A.
Published: March 2020

Young adults (YA) are in a critical stage of life for the encouragement of healthy behaviours such as healthy eating and exercising. This research explored Australian YA values and perceptions related to health, healthy behaviours and health promotion efforts. This qualitative analysis involved n = 166, Australian 18–24 year-olds recruited through a market research field house. Participants (63% currently studying at tertiary level) engaged in a facilitated in-depth online conversation around health and healthy eating over four weeks. LeximancerTM and manual inductive thematic coding were utilised for analysis via the lens of emerging adulthood and health communication theories. Health was seen as multi-faceted, with particular importance placed on mental health and exercise. Some participants focussed on physical appearance, often fuelled by comparison to others. Many believed that at their age and health status, adopting health-enhancing behaviours without short-term tangible benefits was not a priority. Participants did not prioritise health-enhancing behaviours due to barriers such as a perceived lack of money, knowledge and time often due to studying or working and perceived effort. Strategies they proposed to encourage healthy eating included: incentivising healthy food; quick and affordable healthy recipes; and communicating the short-term tangible benefits of healthy behaviours. There is a need for focussed health messaging that address the needs and desires of YA and directly address the barriers they face.
Bifidobacterium animalis subsp. lactis 420 for Metabolic Health: Review of the Research
Authors: Uusitupa Henna-Maria , Rasinkangas Pia , Lehtinen Markus J. , Mäkelä Sanna M. , Airaksinen Kaisa , Anglenius Heli , Ouwehand Arthur C. , Maukonen Johanna
Published: March 2020

The growing worldwide epidemic of obesity and associated metabolic health comorbidities has resulted in an urgent need for safe and efficient nutritional solutions. The research linking obesity with gut microbiota dysbiosis has led to a hypothesis that certain bacterial strains could serve as probiotics helping in weight management and metabolic health. In the search for such strains, the effect of Bifidobacterium animalis subsp. lactis 420 (B420) on gut microbiota and metabolic health, and the mechanisms of actions, has been investigated in a variety of in vitro, pre-clinical, and clinical studies. In this review, we aim to highlight the research on B420 related to obesity, metabolic health, and the microbiota. Current research supports the hypothesis that gut dysbiosis leads to an imbalance in the inflammatory processes and loss of epithelial integrity. Bacterial components, like endotoxins, that leak out of the gut can invoke low-grade, chronic, and systemic inflammation. This imbalanced state is often referred to as metabolic endotoxemia. Scientific evidence indicates that B420 can slow down many of these detrimental processes via multiple signaling pathways, as supported by mechanistic in vitro and in vivo studies. We discuss the connection of these mechanisms to clinical evidence on the effect of B420 in controlling weight gain in overweight and obese subjects. The research further indicates that B420 may improve the epithelial integrity by rebalancing a dysbiotic state induced by an obesogenic diet, for example by increasing the prevalence of lean phenotype microbes such as Akkermansia muciniphila. We further discuss, in the context of delivering the health benefits of B420: the safety and technological aspects of the strain including genomic characterization, antibiotic resistance profiling, stability in the product, and survival of the live probiotic in the intestine. In summary, we conclude that the clinical and preclinical studies on metabolic health suggest that B420 may be a potential candidate in combating obesity; however, further clinical studies are needed.
Consumer Acceptance of Biscuits Supplemented with a Sorghum–Insect Meal
Authors: Awobusuyi Temitope D. , Pillay Kirthee , Siwela Muthulisi
Published: March 2020

Insects are abundant in the predominantly sub-Saharan Africa region and are generally high in protein. Wheat grain contains gluten that is vital for the quality of baked goods but does not grow well in warm regions. Partial substitution of wheat with sorghum and insect in biscuits could contribute to food security among vulnerable populations. This study identified insect types most commonly consumed by the rural Olugboja community living in the rural part of the Ikare-Akoko local government area of Ondo State, Nigeria and consumer acceptance of biscuits supplemented with a sorghum and insect meal. Whole grain sorghum meal and insect meal were blended at a ratio of 3:1 (w/w sorghum: insect). Composite biscuits were made by partially substituting wheat flour with the sorghum–insect meal at 20%, 40%, and 60% (w/w). Wheat biscuit (100%) was used as a control. Regular consumers of biscuits (n = 84) evaluated the acceptability of the biscuit samples using a five-point facial hedonic scale, which was followed by focus group discussions (FGDs) to assess consumer perceptions of the use of insect as a food source. Biscuits containing the sorghum–insect meal (mean = 4.0 ± 0.6) were more acceptable than the control (3.58 ± 0.6). The biscuits supplemented with 20% of the sorghum–insect meal were the most acceptable (mean = 4.23 ± 0.6) compared to those with higher concentrations (40% and 60%). FGDs revealed that the taste of the biscuits was an important motivation for consumers to accept insect as a food source.
In Vitro Anti-NTHi Activity of Haemophilin-Producing Strains of Haemophilus haemolyticus
Authors: Atto Brianna , Latham Roger , Kunde Dale , Gell David A , Tristram Stephen
Published: March 2020

Nontypeable Haemophilus influenzae (NTHi) is a leading causative organism of opportunistic respiratory tract infections. However, there are currently no effective vaccination strategies, and existing treatments are compromised by antibiotic resistance. We previously characterized Haemophilus haemolyticus (Hh) strains capable of producing haemophilin (HPL), a heme-binding protein that restricts NTHi growth by limiting its access to an essential growth factor, heme. Thus, these strains may have utility as a probiotic therapy against NTHi infection by limiting colonization, migration and subsequent infection in susceptible individuals. Here, we assess the preliminary feasibility of this approach by direct in vitro competition assays between NTHi and Hh strains with varying capacity to produce HPL. Subsequent changes in NTHi growth rate and fitness, in conjunction with HPL expression analysis, were employed to assess the NTHi-inhibitory capacity of Hh strains. HPL-producing strains of Hh not only outcompeted NTHi during short-term and extended co-culture, but also demonstrated a growth advantage compared with Hh strains unable to produce the protein. Additionally, HPL expression levels during competition correlated with the NTHi-inhibitory phenotype. HPL-producing strains of Hh demonstrate significant probiotic potential against NTHi colonization in the upper respiratory tract, however, further investigations are warranted to demonstrate a range of other characteristics that would support the eventual development of a probiotic.
Hybrid Overlap Filter for LiDAR Point Clouds Using Free Software
Authors: Buján Sandra , Cordero Miguel , Miranda David
Published: March 2020

Despite the large amounts of resources destined to developing filtering algorithms of LiDAR point clouds in order to obtain a Digital Terrain Model (DTM), the task remains a challenge. As a society advancing towards the democratization of information and collaborative processes, the researchers should not only focus on improving the efficacy of filters, but should also consider the users’ needs with a view toward improving the usability and accessibility of the filters in order to develop tools that will provide solutions to the challenges facing this field of study. In this work, we describe the Hybrid Overlap Filter (HyOF), a new filtering algorithm implemented in the free R software environment. The flow diagram of HyOF differs in the following ways from that of other filters developed to date: (1) the algorithm is formed by a combination of sequentially operating functions (i.e., the output of the first function provides the input of the second), which are capable of functioning independently and thus enabling integration of these functions with other filtering algorithms; (2) the variable penetrability is defined and used, along with slope and elevation, to identify ground points; (3) prior to selection of the seed points, the original point cloud is processed with the aim of removing points corresponding to buildings; and (4) a new method based on a moving window, with longitudinal overlap between windows and transverse overlap between passes, is used to select the seed points. Our hybrid filtering method is tested using 15 reference samples acquired by the International Society of Photogrammetry and Remote Sensing (ISPRS) and is evaluated in comparison with 33 existing filtering algorithms. The results show that our hybrid filtering method produces an average total error of 3.34% and an average Kappa coefficient of 92.62%. The proposed algorithm is one of the most accurate filters that has been tested with the ISPRS reference samples.
On-Orbit Calibration of Installation Parameter of Multiple Star Sensors System for Optical Remote Sensing Satellite with Ground Control Points
Authors: Wang Yanli , Wang Mi , Zhu Ying
Published: March 2020

Owing to the vibrations and thermal shocks that arise during the launch and orbit penetration process, the on-orbit installation parameters of multiple star sensors are different from the on-ground measured parameters, causing inconsistencies in the attitude determinations from different combination modes and seriously affecting the geometric accuracy of high-resolution optical remote sensing images. This study presents an on-orbit calibration approach for the installation parameters of a multiple star sensors system using ground control points (GCPs). Based on the on-ground installation parameters of the optical axes of conventional star sensors, a fiducial coordinate system is proposed as the calibration coordinate system. The installation parameters of the conventional star sensors are calibrated using the statistical characteristics of angles between axes of the star sensor and three fiducial vectors in the J2000 celestial coordinate system. Based on the GCPs, the relative fiducial parameters are calculated, and the installation parameter of unconventional star sensor is then calibrated with the relative fiducial parameters and statistical characteristics of angles. It can be used for high-resolution optical remote sensing satellite measuring with only two star sensors to unify the fiducial coordinate system. The proposed method is tested using simulated data and on-orbit measurement data. The results demonstrate that the proposed method can calibrate the optical axis of the star sensor without the restriction of the accuracy of horizontal axis. Moreover, the star sensor with a large installation angle error can be calibrated well using the proposed approach. The results of attitude determinations from different star sensor combination modes are consistent, and the geometric accuracy of the remote sensing images is significantly improved.
Variability of Kuroshio Surface Axis Northeast of Taiwan Island Derived from Satellite Altimeter Data
Authors: Zhuang Zhanpeng , Zheng Quanan , Zhang Xi , Yang Guangbing , Zhao Xinhua , Cao Lei , Zhang Ting , Yuan Yeli
Published: March 2020

The spatial and temporal variability of the Kuroshio surface axis northeast of Taiwan Island is investigated using 24 years of surface geostrophic currents derived from satellite altimeter data from 1993 to 2016. The Kuroshio surface axis is derived by an extraction method with three selected parameters, including the length of the subsidiary line, the intervals between two adjacent points, and the distance between the two adjacent subsidiary lines. The empirical mode decomposition analysis on the 24-year Kuroshio axes reveals that the mean periods of intra-seasonal and inter-annual variability, which are the two dominant components, are about 3.2 months and 1.3 years, respectively. The self-organizing map analysis reveals that the variation of Kuroshio axis northeast of Taiwan Island has four best matching unit (BMU) patterns: straight-path (BMUS), meandering-path (BMUM) and two transition stages (BMUT1 and BMUT2). The straight-path pattern shows strong seasonality: more likely occurring in summer. The meandering-path pattern is less frequent than straight-path pattern. During a typical period from November 26, 2012 to January 27, 2013, which is chosen as an independent example, the analysis on the satellite altimeter and sea surface temperature data shows that the patterns of the Kuroshio axis change successively in order of BMUT1→BMUM→BMUT2→BMUS, i.e., the Kuroshio axis migrates from the meandering-path to the straight-path pattern. During the typical period the warm water intrusion and a mesoscale eddy occur at the second stage corresponding to BMUM and migrate northwestward gradually at the last two stages corresponding to BMUT2 and BMUS. The transient order appears only during this typical period but it is not common for the whole study period. The monthly mean relatively vorticity is calculated and analyzed to evaluate the impact of the eddies on the Kuroshio surface axis variability, the results show that the anticyclonic (cyclonic) eddies can promote the Kuroshio surface axis to present the meandering-path (straight-path) pattern because of the potential vorticity conservation. The impacts of the anticyclonic eddies and the cyclonic eddies on the variability of the Kuroshio surface axis are opposite. The long-term day-to-day detection contributes to improving understanding the variability of Kuroshio surface axis northeast of Taiwan Island.
Regional Differences in Fossil Energy-Related Carbon Emissions in China’s Eight Economic Regions: Based on the Theil Index and PLS-VIP Method
Authors: Liu Xianzhao , Yang Xu , Guo Ruoxin
Published: March 2020

Determining differences in regional carbon emissions and the factors that affect these differences is important in the realization of differentiated emissions mitigation policies. This paper adopts the Theil index and the partial least square-variable importance of projection (PLS-VIP) method to analyze the change characteristics, regional differences and causes of carbon emissions, as well as the extent to which various factors influenced carbon emissions in China’s eight economic regions in 2005–2017. The results indicate that (1) during the study period, carbon emissions in the eight economic regions displayed a rigid uptrend with a phased characteristic. The growth rates of carbon emissions were different across the studied regions. (2) The overall difference in regional carbon emissions showed an increasing trend, mainly owing to increasing interregional differences. (3) The extent of the influence and explanatory ability of each factor on regional carbon emissions and discrepancies in carbon emissions were different. Population size, economic development, and energy intensity were found to be the three main factors influencing regional carbon emission changes. Industrial structure and urbanization were also contributors to regional differences in emissions. The influence of energy structure on regional carbon emissions and its explanatory power were weak on the whole, but its elastic coefficients and VIP values changed significantly. Finally, regionally targeted proposals for emissions mitigation are offered.
Analysis of Using Biogas Resources for Electric Vehicle Charging in Bangladesh: A Techno-Economic-Environmental Perspective
Authors: Karmaker Ashish Kumar , Hossain Md. Alamgir , Manoj Kumar Nallapaneni , Jagadeesan Vishnupriyan , Jayakumar Arunkumar , Ray Biplob
Published: March 2020

The growing popularity of electric vehicles (EV) is creating an increasing burden on the power grid in Bangladesh due to massive energy consumption. Due to this uptake of variable energy consumption, environmental concerns, and scarcity of energy lead to investigate alternative energy resources that are readily available and environment friendly. Bangladesh has enormous potential in the field of renewable resources, such as biogas and biomass. Therefore, this paper proposes a design of a 20 kW electric vehicle charging station (EVCS) using biogas resources. A comprehensive viability analysis is also presented for the proposed EVCS from technological, economic, and environmental viewpoints using the HOMER (Hybrid Optimization of Multiple Energy Resources) model. The viability result shows that with the capacity of 15–20 EVs per day, the proposed EVCS will save monthly $16.31 and $29.46, respectively, for easy bike and auto-rickshaw type electric vehicles in Bangladesh compare to grid electricity charging. Furthermore, the proposed charging station can reduce 65.61% of CO2 emissions than a grid-based charging station.
Biophysical Economics for Policy and Teaching: Mexico as an Example
Authors: Peniche Camps Salvador , Hall Charles A. S. , Klitgaard Kent
Published: March 2020

Many parts of the world are currently facing unprecedented social turmoil. Few understand that most of these “exploding” situations have a biophysical basis in patterns of consumption and the ratio of number of humans to resources available. Most “solutions” proposed are political oppression or, for the lucky, economic development, usually led by conventional economists. However, we believe that, for many regions, conventional economics, certainly alone and perhaps in their entirety, are not up to the job of addressing these crises. We propose a new discipline, Biophysical Economics, that addresses these lacunae and offers a good set of procedures for bringing much more natural science to the discipline of economics. This approach provides a stronger basis for training young people in both economics and heterodox political economy. We will need economists with this new training for a future that appears very different from today. This article outlines the rationales for further developing and teaching Biophysical Economics to demonstrate its utility and applies this economic lens to the economy of Mexico. We finish by providing an example of how a Biophysical Economics curriculum appropriate to analyzing and addressing the Mexican economic context might be developed and taught at the University of Guadalajara. This curriculum could also be adapted to other national, educational and institutional settings.
Suitable Scale of an Oasis in Different Scenarios in an Arid Region of China: A Case Study of the Ejina Oasis
Authors: Ye Zhaoxia , Fu Aihong , Zhang Shuhua , Yang Yuhai
Published: March 2020

The main objective of this work is to calculate the suitable scale of the Ejina Oasis in different scenarios (high-, normal-, and low-flow years), assess the stability of the Ejina Oasis, and, finally, accurately determine if an artificial oasis area and total oasis area need to be reduced or increased. Using the water balance method, we calculated the suitable scale of the artificial Ejina Oasis as 767.80, 624.00, and 451.33 km2 in high-, normal-, and low-flow years, respectively. By utilizing the water-heat balance method combined with an assessment of the present stability of the Ejina Oasis, the results showed that in high-flow years, the suitable scale of the Ejina Oasis is 1174–1762 km2, and the stability index of the current oasis is 0.55. In normal-flow years, the suitable scale of the Ejina Oasis is found to be 915–1373 km2, and the stability index of the current oasis is 0.43. In low-flow years, the suitable scale of the Ejina Oasis is 590–885 km2, and the stability index of the current oasis decreases to 0.27. In order to further improve the stability of oases, it remains necessary to control the scale of oases to cope with water resource shortages that result from water resource instability under climate change. The present study’s findings will enable optimal water-use planning decisions that take social, economic, and ecological issues into account, and provide the foundation for optimal decision-making for regional administrative departments to effectively coordinate regional economic development, farmland protection, environmental protection, and water resource protection.
Does Sustainable Corporate Governance Enhance Accounting Practice? Evidence from the Korean Market
Authors: Choi Daeheon , Choi Paul Moon Sub , Choi Joung Hwa , Chung Chune Young
Published: March 2020

As corporate sustainability continues to improve and enhance the principles of good corporate governance, firms are exerting increasing efforts in terms of transparency and public disclosure. Transparency efforts provide information to the general public on the relationship between corporate governance and improved sustainability. The better informed shareholders are about the connection between corporate governance and sustainability, the more apparent the relationship will become over time. Prior studies assume that blockholders engage in active institutional monitoring by intervening directly in firms’ operations. In contrast, we argue that passive institutional monitoring is a more feasible governance mechanism in the Korean market owing to the market’s unique features (i.e., chaebols and pressure sensitivity). In particular, focusing on the blockholdings of the Korean National Pension Service (KNPS), we study the impact of passive monitoring on firms’ earnings quality, represented by earnings persistence, value relevance, and timeliness. The empirical evidence shows that KNPS blockholdings have a positive and significant impact on corporate earnings quality, indicating that passive blockholder monitoring is a more efficient channel for improving earnings quality in South Korea. Our results may be generalized to other emerging markets in which a few entities with concentrated economic power engender pressure-sensitive corporate landscapes for sustainability.