bluuebunny/crossref_metadata_2025 huggingface dataset:
MicroRNAs Instruct and Maintain Cell Type Diversity in the Nervous System
Authors: Zolboot Norjin , Du Jessica X. , Zampa Federico , Lippi Giordano
Published: April 2021

Characterizing the diverse cell types that make up the nervous system is essential for understanding how the nervous system is structured and ultimately how it functions. The astonishing range of cellular diversity found in the nervous system emerges from a small pool of neural progenitor cells. These progenitors and their neuronal progeny proceed through sequential gene expression programs to produce different cell lineages and acquire distinct cell fates. These gene expression programs must be tightly regulated in order for the cells to achieve and maintain the proper differentiated state, remain functional throughout life, and avoid cell death. Disruption of developmental programs is associated with a wide range of abnormalities in brain structure and function, further indicating that elucidating their contribution to cellular diversity will be key to understanding brain health. A growing body of evidence suggests that tight regulation of developmental genes requires post-transcriptional regulation of the transcriptome by microRNAs (miRNAs). miRNAs are small non-coding RNAs that function by binding to mRNA targets containing complementary sequences and repressing their translation into protein, thereby providing a layer of precise spatial and temporal control over gene expression. Moreover, the expression profiles and targets of miRNAs show great specificity for distinct cell types, brain regions and developmental stages, suggesting that they are an important parameter of cell type identity. Here, we provide an overview of miRNAs that are critically involved in establishing neural cell identities, focusing on how miRNA-mediated regulation of gene expression modulates neural progenitor expansion, cell fate determination, cell migration, neuronal and glial subtype specification, and finally cell maintenance and survival.
Eukaryotic Translation Initiation Factor 3 Subunit B Is a Promoter in the Development and Progression of Pancreatic Cancer
Authors: Ren Haoyuan , Mai Gang , Liu Yong , Xiang Rongchao , Yang Chong , Su Wenjie
Published: April 2021

Background: Pancreatic cancer (PC) is a malignant tumor with hidden incidence, high degree of malignancy, rapid disease progression, and poor prognosis. Eukaryotic translation initiation factor 3 subunit B (EIF3B) is necessary for tumor growth, which is an alternative therapeutic target for many cancers. However, little is known about the relationship between EIF3B and PC.

Methods: The expression of EIF3B in PC was detected by immunohistochemistry. EIF3B knockdown cell models were constructed by lentivirus infection. The MTT assay, the wound-healing assay, the transwell assay, the flow cytometry, and the Human Apoptosis Antibody Array was used to detect the effects of EIF3B knockdown on cell proliferation, cell migration, cell apoptosis, and cell cycle in vitro. Also, the effects of EIF3B knockdown on the tumor growth of PC were determined in vivo.

Results: This study showed that the expression level of EIF3B was significantly up-regulated in PC tumor tissues and associated with pathological grade. In vitro, EIF3B knockdown inhibited the PC cell proliferation and migration, and the apoptosis levels were obviously promoted by regulating apoptosis-related proteins including Bcl-2, HSP27, HSP60, Survivin, sTNF-R2, TNF-α, TNF-β, TRAILR-3, TRAILR-4, and XIAP. Furthermore, the tumor growth of PC was inhibited after the knockdown of EIF3B in vivo.

Conclusion: EIF3B was up-regulated in PC and was a promoter in the development and progression of PC, which could be considered as a therapeutic target for the treatment of PC.
Zanubrutinib Treatment of Central Nervous System Posttransplant Lymphoproliferative Disorder After Allogeneic Hematopoietic Stem Cell Transplantation: A Case Report
Authors: Yang Ting-Ting , Chen Wei-Hao , Zhao Yan-Min , Fu Hua-Rui , Huang He , Shi Ji-Min
Published: April 2021

Posttransplant lymphoproliferative disorder (PTLD) is a rare complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT) with poor prognosis. We report a patient with PTLD involved central nervous system (CNS) who treated with zanubrutinib, a second-generation Bruton tyrosine kinase (BTK) inhibitor. Our report supports the efficacy of bruton tyrosine kinase inhibitor zanubrutinib in the treatment of CNS-PTLD, which provides a new therapeutic option.
Inverse Mechanistic Modeling of Transdermal Drug Delivery for Fast Identification of Optimal Model Parameters
Authors: Defraeye Thijs , Bahrami Flora , Rossi René M.
Published: April 2021

Transdermal drug delivery systems are a key technology to administer drugs with a high first-pass effect in a non-invasive and controlled way. Physics-based modeling and simulation are on their way to become a cornerstone in the engineering of these healthcare devices since it provides a unique complementarity to experimental data and additional insights. Simulations enable to virtually probe the drug transport inside the skin at each point in time and space. However, the tedious experimental or numerical determination of material properties currently forms a bottleneck in the modeling workflow. We show that multiparameter inverse modeling to determine the drug diffusion and partition coefficients is a fast and reliable alternative. We demonstrate this strategy for transdermal delivery of fentanyl. We found that inverse modeling reduced the normalized root mean square deviation of the measured drug uptake flux from 26 to 9%, when compared to the experimental measurement of all skin properties. We found that this improved agreement with experiments was only possible if the diffusion in the reservoir holding the drug was smaller than the experimentally measured diffusion coefficients suggested. For indirect inverse modeling, which systematically explores the entire parametric space, 30,000 simulations were required. By relying on direct inverse modeling, we reduced the number of simulations to be performed to only 300, so a factor 100 difference. The modeling approach’s added value is that it can be calibrated once in-silico for all model parameters simultaneously by solely relying on a single measurement of the drug uptake flux evolution over time. We showed that this calibrated model could accurately be used to simulate transdermal patches with other drug doses. We showed that inverse modeling is a fast way to build up an accurate mechanistic model for drug delivery. This strategy opens the door to clinically ready therapy that is tailored to patients.
Cichorium pumilum Jacq Extract Inhibits LPS-Induced Inflammation via MAPK Signaling Pathway and Protects Rats From Hepatic Fibrosis Caused by Abnormalities in the Gut-Liver Axis
Authors: Han Chang , Wu Xi , Zou Nan , Zhang Yunsheng , Yuan Jinqi , Gao Yuefeng , Chen Wen , Yao Jia , Li Cong , Hou Jinqiu , Qin Dongmei
Published: April 2021

The development of liver fibrosis is closely related to the gut microbiota, and the “gut-liver axis” is the most important connection between the two. ethyl acetate extract of Cichorium pumilum Jacq (CGEA) is an herbal extract consisting mainly of sesquiterpenoids. The anti-inflammatory and hepatoprotective effects of CGEA have been reported, but the anti-fibrotic effects of CGEA via intestinal microbes and the “gut-liver axis” cycle have rarely been reported. In this study, we observed that CGEA not only directly attenuated inflammatory factor levels in inflamed mice, but also attenuated liver inflammation as well as liver fibrosis degeneration in rats with liver fibrosis caused by colitis. We observed in vitro that CGEA significantly promoted the growth of Bifidobacterium adolescentis. Similarly, fecal 16S rDNA sequencing of liver fibrosis rats showed that CGEA intervention significantly altered the composition of the intestinal microbiota of liver fibrosis rats. CGEA increased the abundance of intestinal microbiota, specifically, CGEA increased the ratio of Firmicutes to Bacteroidetes, CGEA could significantly increase the levels of Ruminococcus. In addition, CGEA intervention significantly protected intestinal mucosal tissues and improved intestinal barrier function in rats. Lactucin is the main sesquiterpenoid in CGEA, and HPLC results showed its content in CGEA was up to 6%. Lactucin has been reported to have significant anti-inflammatory activity, and in this study, we found that Lactucin decreased p38 kinases (p38), phosphorylation of the extracellular signal-regulated kinase (ERK) and protein kinase B (AKT) protein phosphorylation in lipopolysaccharide (LPS)-activated RAW264.7 cells, thereby reducing mRNA expression and protein expression of pro-inflammatory factors inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and inhibiting the release of inflammatory factors interleukin (IL)-6 and nitric oxide (NO), exerting anti-inflammatory effects. In summary, the prevention of liver fibrosis caused by intestinal inflammation by CGEA may be achieved by regulating the intestinal microbiota and restoring the intestinal barrier thereby improving the “gut-liver axis” circulation, reducing liver inflammation, and ultimately alleviating liver fibrosis. Notably, the direct anti-inflammatory effect of CGEA may be due to its content of Lactucin, which can exert anti-inflammatory effects by inhibiting the phosphorylation of Mitogen-activated protein kinase (MAPK) and Akt signaling pathways.
Psychological Impact of COVID-19 on College Students After School Reopening: A Cross-Sectional Study Based on Machine Learning
Authors: Ren Ziyuan , Xin Yaodong , Ge Junpeng , Zhao Zheng , Liu Dexiang , Ho Roger C. M. , Ho Cyrus S. H.
Published: April 2021

COVID-19, the most severe public health problem to occur in the past 10 years, has greatly impacted people's mental health. Colleges in China have reopened, and how to prevent college students from suffering secondary damage due to school reopening remains elusive. This cross-sectional study was aimed to evaluate the psychological impact of COVID-19 after school reopening and explore via machine learning the factors that influence anxiety and depression among students. Among the 478 valid online questionnaires collected between September 14th and September 20th, 74 (15.5%) showed symptoms of anxiety (by the Self-Rating Anxiety Scale), and 155 (32.4%) showed symptoms of depression (by Patient Health Questionnaire-9). Descriptive analysis of basic personal characteristics indicated that students at a higher grade, having relatives or friends who have been infected, fearing being infected, and having a pessimistic attitude to COVID-19 easily experience anxiety or depression. The Synthetic Minority Oversampling Technique (SMOTE) was utilized to counteract the imbalance of retrieved data. The Akaike Information Criterion (AIC) and multivariate logistic regression were performed to explore significant influence factors. The results indicate that exercise frequency, alcohol use, school reopening, having relatives or friends who have been infected, self-quarantine, quarantine of classmates, taking temperature routinely, wearing masks routinely, sleep quality, retaining holiday, availability of package delivery, take-out availability, lockdown restriction, several areas in school closed due to COVID-19, living conditions in the school, taking the final examinations after school reopening, and the degree to which family economic status is influenced by COVID-19 are the primary influence factors for anxiety or depression. To evaluate the effect of our model, we used 5-fold cross-validation, and the average area under the curve (AUC) values of the receiver operating characteristic (ROC) curves of anxiety and depression on the test set reached 0.885 and 0.806, respectively. To conclude, we examined the presence of anxiety and depression symptoms among Chinese college students after school reopening and explored many factors influencing students' mental health, providing reasonable school management suggestions.
Cardiovascular Risk of Concomitant Use of Atypical Antipsychotics and Stimulants Among Commercially Insured Youth in the United States
Authors: Zhang Chengchen , Spence O'Mareen , Reeves Gloria , DosReis Susan
Published: April 2021

Objectives: To investigate the risk of cardiovascular events associated with concomitant use of stimulants and atypical antipsychotics (AAPs) among youth and evaluate whether AAP dose and duration of concomitant use modifies the risk.

Methods: We used IQVIA PharMetrics® Plus data from 2006 to 2015 to construct a retrospective cohort of commercially-insured youth aged 5–17 years old who initiated a stimulant medication. Time-varying concomitant stimulant/AAP use was defined as current, past and no concomitant use based on person months. The primary time-varying Cox proportional hazard regression analysis evaluated the risk of cardiovascular events comparing current concomitant use with past and no concomitant use, adjusted for baseline cardiovascular risk. A secondary analysis assessed the risk of cardiovascular events comparing AAP daily doses (<1, 1–2, >2 mg) and duration (<3, 3–6, >6 months) of current concomitant use to no concomitant use. Cardiovascular outcomes included severe (i.e., stroke, acute myocardial infarction, ischemic heart disease) and less severe (i.e., angina pectoris, cardiac dysrhythmias, transient cerebral ischemia, hypertensive disease, tachycardia, palpitations, syncope).

Results: For this cohort of 61,438 youths, the incidence rate of severe cardiovascular events was 0.18 per 10,000 person-months, and all events occurred in no concomitant use months. The risk of less severe cardiovascular events was significantly higher in current concomitant users compared with no [HR: 2.59 (95%CI: 1.72, 3.90)] and past [HR: 1.89 (95%CI: 1.10, 3.24)] concomitant users. Compared to no concomitant use, the risk of less severe cardiovascular events was significantly higher at all AAP daily doses [HR: <1 mg: 2.82 (95%CI: 1.72, 4.61); 1–2 mg: 2.22 (95%CI: 1.16, 4.25); >2 mg: 2.65 (95%CI: 1.50, 4.71)]. The risk of less severe cardiovascular events significantly elevated for all duration of use and was higher for <3 months of concomitant use [HR: <3 months: 3.45 (95%CI: 2.17, 5.47) relative to 3–6 months: 2.60 (95%CI: 1.29, 5.25) or >6 months: 2.61 (95%CI: 1.59, 4.30)].

Conclusions: Severe cardiovascular events are rare. Concomitant stimulant/AAP use elevates the risk of less severe cardiovascular events. Periodic heart rate or blood pressure monitoring for youth on stimulant/AAP treatment may be warranted.
Seroprevalence of SARS-CoV-2 Assessed by Four Chemiluminescence Immunoassays and One Immunocromatography Test for SARS-Cov-2
Authors: Cerino Pellegrino , Gallo Alfonso , Pierri Biancamaria , Buonerba Carlo , Di Concilio Denise , Cuomo Maria Concetta , Vassallo Lucia , Lo Conte Gabriella , Coppola Annachiara , Pizzolante Antonio , Boccia Giovanni , Ferrucci Veronica , Atripaldi Luigi , Triassi Maria , Pacella Daniela , Cennamo Michele , Romano Paolo , Sorbo Teresa Maria , Furno Alessandro , Catapano Oriana , Contina Aldo , Perruolo Giuseppe , D'Amora Maurizio , Terracciano Daniela , Portella Giuseppe
Published: April 2021

The onset of the new SARS-CoV-2 coronavirus encouraged the development of new serologic tests that could be additional and complementary to real-time RT-PCR-based assays. In such a context, the study of performances of available tests is urgently needed, as their use has just been initiated for seroprevalence assessment. The aim of this study was to compare four chemiluminescence immunoassays and one immunochromatography test for SARS-Cov-2 antibodies for the evaluation of the degree of diffusion of SARS-CoV-2 infection in Salerno Province (Campania Region, Italy). A total of 3,185 specimens from citizens were tested for anti-SARS-CoV-2 antibodies as part of a screening program. Four automated immunoassays (Abbott and Liaison SARS-CoV-2 CLIA IgG and Roche and Siemens SARS-CoV-2 CLIA IgM/IgG/IgA assays) and one lateral flow immunoassay (LFIA Technogenetics IgG–IgM COVID-19) were used. Seroprevalence in the entire cohort was 2.41, 2.10, 1.82, and 1.85% according to the Liaison IgG, Abbott IgG, Siemens, and Roche total Ig tests, respectively. When we explored the agreement among the rapid tests and the serologic assays, we reported good agreement for Abbott, Siemens, and Roche (Cohen's Kappa coefficient 0.69, 0.67, and 0.67, respectively), whereas we found moderate agreement for Liaison (Cohen's kappa coefficient 0.58). Our study showed that Abbott and Liaison SARS-CoV-2 CLIA IgG, Roche and Siemens SARS-CoV-2 CLIA IgM/IgG/IgA assays, and LFIA Technogenetics IgG-IgM COVID-19 have good agreement in seroprevalence assessment. In addition, our findings indicate that the prevalence of IgG and total Ig antibodies against SARS-CoV-2 at the time of the study was as low as around 3%, likely explaining the amplitude of the current second wave.
3D Facial Pain Expression for a Care Training Assistant Robot in an Elderly Care Education Environment
Authors: Lee Miran , Tran Dinh Tuan , Lee Joo-Ho
Published: April 2021

As the elderly population increases, the importance of the caregiver’s role in the quality of life of the elderly has increased. To achieve effective feedback in terms of care and nursing education, it is important to design a robot that can express emotions or feel pain like an actual human through visual-based feedback. This study proposes a care training assistant robot (CaTARo) system with 3D facial pain expression that simulates an elderly person for improving the skills of workers in elderly care. First, in order to develop an accurate and efficient system for elderly care training, this study introduces a fuzzy logic–based care training evaluation method that can calculate the pain level of a robot for giving the feedback. Elderly caregivers and trainees performed the range of motion exercise using the proposed CaTARo. We obtained quantitative data from CaTARo, and the pain level was calculated by combining four key parameters using the fuzzy logic method. Second, we developed a 3D facial avatar for use in CaTARo that is capable of expressing pain based on the UNBC-McMaster Pain Shoulder Archive, and we then generated four pain groups with respect to the pain level. To mimic the conditions for care training with actual humans, we designed the system to provide pain feedback based on the opinions of experts. The pain feedback was expressed in real time by using a projector and a 3D facial mask during care training. The results of the study confirmed the feasibility of utilizing a care training robot with pain expression for elderly care training, and it is concluded that the proposed approach may be used to improve caregiving and nursing skills upon further research.
A Postphenomenological Framework for Studying User Experience of Immersive Virtual Reality
Authors: Vindenes Joakim , Wasson Barbara
Published: April 2021

Virtual Reality (VR) is a remarkably flexible technology for interventions as it allows the construction of virtual worlds with ontologies radically different from the real world. By embodying users in avatars situated in these virtual environments, researchers can effectively intervene and instill positive change in the form of therapy or education, as well as affect a variety of cognitive changes. Due to the capabilities of VR to mediate both the environments in which we are immersed, as well as our embodied, situated relation toward those environments, VR has become a powerful technology for “changing the self.” As the virtually mediated experience is what renders these interventions effective, frameworks are needed for describing and analyzing the mediations brought by various virtual world designs. As a step toward a broader understanding of how VR mediates experience, we propose a post-phenomenological framework for describing VR mediation. Postphenomenology is a philosophy of technology concerned with empirical data that understand technologies as mediators of human-world relationships. By addressing how mediations occurwithinVR as a user-environment relation andoutsideVR as a human-world relation, the framework addresses the various constituents of the virtually mediated experience. We demonstrate the framework's capability for describing VR mediations by presenting the results of an analysis of a selected variety of studies that use various user-environment relations to mediate various human-world relations.
Indian Diaspora Gastronomy: On the Changing Use of Herbs and Spices Among Southern California's Indian Immigrant Women
Authors: Joseph Elois , Voeks Robert
Published: April 2021

Southern California has witnessed a burgeoning Indian immigrant population in recent decades. And among the cultural features that most distinguishes Indians is their cuisine. Their use of herbs and spices in food and medicine, in particular, is tightly bound to language, religion, gender, and overall cultural identity. Identifying how Indian immigrants' culinary choices adapt to southern California's varied and often fast-food based gastronomy, particularly impacts on the inter-generational transmission of traditional culinary knowledge, is important in terms of understanding the role of cultural retention and assimilation, as well as culturally-defined notions of food in physical and psychological well-being. We explored these questions by means of interviews with 31 Indian immigrant women in southern California. Participants were selected by means of snowball sampling. Our working hypothesis was that problems with sourcing and cultural assimilation pressures would have eroded the use of traditional herbs and spices. A total of 66 herbs and spices (and associated seasonings) were reported. Of these, the highest frequency of use was recorded for turmeric (100% of respondents) followed by cilantro, cinnamon, clove, cumin, curry leaves, and ginger (all 97%). The highest Species Medicinal Use Values were recorded, in descending order, for turmeric, ginger, fenugreek seeds, clove, cinnamon, curry leaves, and Tulsi. Contrary to expectations, there was no significant association between years resident in the United States and decreasing use of herbs and spices. Indeed, in some cases the confluence of northern and southern Indian immigrant women with a new identity simply as “Indian” resulted in an increase in the knowledge and use of herbs and spices. Spices are nearly all locally sourced, and where specific herbs are not readily accessible, they are cultivated in homegardens or brought directly from India. Many Indian immigrants are relatively prosperous and able to travel frequently to and from India, thus maintaining close cultural ties with their homeland. Indian immigrant women are fully aware of the health benefits associated with the use of traditional herbs and spices, and all participants reported that Indian food is a healthier choice than American cuisine. Knowledge is passedviavertical transmission, primarily through mothers and grandmothers to daughters. Overall, there is little concern among female Indian immigrants to southern California that knowledge and use of their traditional herbs and spices are in a state of decline.
Recent Developments in Smart Food Packaging Focused on Biobased and Biodegradable Polymers
Authors: Salgado Pablo R. , Di Giorgio Luciana , Musso Yanina S. , Mauri Adriana N.
Published: April 2021

Food packaging has a crucial function in the modern food industry. New food packaging technologies seek to meet consumers and industrial's demands. Changes related to food production, sale practices and consumers' lifestyles, along with environmental awareness and the advance in new areas of knowledge (such as nanotechnology or biotechnology), act as driving forces to develop smart packages that can extend food shelf-life, keeping and supervising their innocuousness and quality and also taking care of the environment. This review describes the main concepts and types of active and intelligent food packaging, focusing on recent progress and new trends using biodegradable and biobased polymers. Numerous studies show the great possibilities of these materials. Future research needs to focus on some important aspects such as possibilities to scale-up, costs, regulatory aspects, and consumers' acceptance, to make these systems commercially viable.
Community Seed Banks in China: Achievements, Challenges and Prospects
Authors: Song Xin , Li Guanqi , Vernooy Ronnie , Song Yiching
Published: April 2021

The rich agrobiodiversity of China is under unprecedented threat, experiencing a dramatic loss of many valuable local varieties and wild relatives of main crops. The country's formal conservation system of ex situ genebanks faces serious challenges to address this loss. Community seed banks can play a key role to complement the conservation activities of these genebanks and provide other important collective goods, such as evolutionary services, but although they have been around for some 35 years in various parts of the world, in China they have a much shorter history. In recent years though the number has increased to almost 30 in 2020, in particular due to the efforts of the China Farmers' Seed Network. The community seed banks in the country are very diverse in terms of functions and services, forms of management and institutional linkages. Compared to the most common functions of community seed banks in other countries, China is bringing an important design innovation through two new functions: adding value to seed and produce through innovative marketing strategies, and building regional and national seed system linkages and fostering collaboration. The review of community seed banking not only provides rich empirical evidence, but also makes an important contribution to theory. Building on the achievements of community seed banking in the last decade, there is scope to scale this kind of very valuable agrobiodiversity conservation approach through more effective uptake and support by relevant national policies.
Research and Development of Microphysiological Systems in Japan Supported by the AMED-MPS Project
Authors: Ishida Seiichi
Published: April 2021

Microphysiological systems (MPS) have been actively developed as a new technology for in vitro toxicity testing platforms in recent years. MPS are culture techniques for the reconstruction of the specific functions of human organs or tissues in a limited space to create miniaturized human test systems. MPS have great promise as next-generation in vitro toxicity assessment systems. Here, I will review the current status of MPS and discuss the requirements that must be met in order for MPS to be implemented in the field of drug discovery, presenting the example of an in vitro cell assay system for drug-induced liver injury, which is the research subject in our laboratory. Projects aimed at the development of MPS were implemented early in Europe and the United States, and the AMED-MPS project was launched in Japan in 2017. The AMED-MPS project involves industry, government, and academia. Researchers in the field of drug discovery in the pharmaceutical industry also participate in the project. Based on the discussions made in the project, I will introduce the requirements that need to be met by liver-MPS as in vitro toxicity test platforms.
Comparative Genome-Wide Alternative Splicing Analysis of Longissimus Dorsi Muscles Between Japanese Black (Wagyu) and Chinese Red Steppes Cattle
Authors: Fang Xibi , Xia Lixin , Yu Haibin , He Wei , Bai Zitong , Qin Lihong , Jiang Ping , Zhao Yumin , Zhao Zhihui , Yang Runjun
Published: April 2021

Alternative splicing is a ubiquitous regulatory mechanism in gene expression that allows a single gene generating multiple messenger RNAs (mRNAs). Significant differences in fat deposition ability and meat quality traits have been reported between Japanese black cattle (Wagyu) and Chinese Red Steppes, which presented a unique model for analyzing the effects of transcriptional level on marbling fat in livestock. In previous studies, the differentially expressed genes (DGEs) in longissimus dorsi muscle (LDM) samples between Wagyu and other breeds of beef cattle have been reported. In this study, we further investigated the differences in alternative splicing in LDM between Wagyu and Chinese Red Steppes cattle. We identified several alternative splicing types including cassette exon, mutually exclusive exons, alternative 5′ splice site, alternative 3′ splice site, alternative start exon, and intron retention. In total, 115 differentially expressed alternatively spliced genes were obtained, of which 17 genes were enriched in the metabolic pathway. Among the 17 genes, 5 genes, including MCAT, CPT1B, HADHB, SIRT2, and DGAT1, appeared to be the novel spliced candidates that affect the lipid metabolism in cattle. Additionally, another 17 genes were enriched in the Gene Ontology (GO) terms related to muscle development, such as NR4A1, UQCC2, YBX3/CSDA, ITGA7, etc. Overall, altered splicing and expression levels of these novel candidates between Japanese black cattle and Chinese Red Steppes revealed by RNA-seq suggest their potential involvement in the muscle development and fat deposition of beef cattle.
20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases
Authors: Dinan Laurence , Dioh Waly , Veillet Stanislas , Lafont Rene
Published: April 2021

There is growing interest in the pharmaceutical and medical applications of 20-hydroxyecdysone (20E), a polyhydroxylated steroid which naturally occurs in low but very significant amounts in invertebrates, where it has hormonal roles, and in certain plant species, where it is believed to contribute to the deterrence of invertebrate predators. Studies in vivo and in vitro have revealed beneficial effects in mammals: anabolic, hypolipidemic, anti-diabetic, anti-inflammatory, hepatoprotective, etc. The possible mode of action in mammals has been determined recently, with the main mechanism involving the activation of the Mas1 receptor, a key component of the renin–angiotensin system, which would explain many of the pleiotropic effects observed in the different animal models. Processes have been developed to produce large amounts of pharmaceutical grade 20E, and regulatory preclinical studies have assessed its lack of toxicity. The effects of 20E have been evaluated in early stage clinical trials in healthy volunteers and in patients for the treatment of neuromuscular, cardio-metabolic or respiratory diseases. The prospects and limitations of developing 20E as a drug are discussed, including the requirement for a better evaluation of its safety and pharmacological profile and for developing a production process compliant with pharmaceutical standards.
A Narrative Review of the Role of Diet and Lifestyle Factors in the Development and Prevention of Endometrial Cancer
Authors: Yasin Hajar Ku , Taylor Anthony H. , Ayakannu Thangesweran
Published: April 2021

Endometrial cancer is the most common cancer affecting the reproductive organs of women living in higher-income countries. Apart from hormonal influences and genetic predisposition, obesity and metabolic syndrome are increasingly recognised as major factors in endometrial cancer risk, due to changes in lifestyle and diet, whereby high glycaemic index and lipid deposition are prevalent. This is especially true in countries where micronutrients, such as vitamins and minerals are exchanged for high calorific diets and a sedentary lifestyle. In this review, we will survey the currently known lifestyle factors, dietary requirements and hormonal changes that increase an individual’s risk for endometrial cancer and discuss their relevance for clinical management. We also examine the evidence that everyday factors and clinical interventions have on reducing that risk, such that informed healthy choices can be made. In this narrative review, we thus summarise the dietary and lifestyle factors that promote and prevent the incidence of endometrial cancer.
Anti-VEGF-Resistant Retinal Diseases: A Review of the Latest Treatment Options
Authors: Wallsh Josh O. , Gallemore Ron P.
Published: April 2021

Anti-vascular endothelial growth factor (anti-VEGF) therapy currently plays a central role in the treatment of numerous retinal diseases, most notably exudative age-related macular degeneration (eAMD), diabetic retinopathy and retinal vein occlusions. While offering significant functional and anatomic benefits in most patients, there exists a subset of 15–40% of eyes that fail to respond or only partially respond. For these cases, various treatment options have been explored with a range of outcomes. These options include steroid injections, laser treatment (both thermal therapy for retinal vascular diseases and photodynamic therapy for eAMD), abbreviated anti-VEGF treatment intervals, switching anti-VEGF agents and topical medications. In this article, we review the effectiveness of these treatment options along with a discussion of the current research into future directions for anti-VEGF-resistant eyes.
Significance of Concurrent Chemoradiotherapy as Primary Treatment in Patients with Metastatic Cervical Cancer
Authors: Hattori Satomi , Yoshikawa Nobuhisa , Mogi Kazumasa , Yoshida Kosuke , Yoshihara Masato , Tamauchi Satoshi , Ikeda Yoshiki , Yokoi Akira , Nishino Kimihiro , Niimi Kaoru , Suzuki Shiro , Kajiyama Hiroaki
Published: April 2021

(1) This study investigated the prognostic impact of tumor size in patients with metastatic cervical cancer. (2) Methods: Seventy-three cervical cancer patients in our institute were stratified into two groups based on distant metastasis: para-aortic lymph node metastasis alone (IIIC2) or spread to distant visceral organs with or without para-aortic lymph node metastasis (IVB) to identify primary tumor size and concurrent chemoradiotherapy. (3) Results: The overall survival (OS) for patients with a tumor >6.9 cm in size was significantly poorer than that for patients with a tumor ≤6.9 cm in the IVB group (p = 0.0028); the corresponding five-year OS rates in patients with a tumor ≤6.9 and >6.9 cm were 53.3% and 13.4%, respectively. In the multivariate analysis, tumor size and primary treatment were significantly associated with survival in metastatic cervical cancer. (4) Conclusions: Tumor size ≤6.9 cm and concurrent chemoradiotherapy as the primary treatment were favorable prognostic factors for patients with metastatic cervical cancer.
Biophotons and Emergence of Quantum Coherence—A Diffusion Entropy Analysis
Authors: Benfatto Maurizio , Pace Elisabetta , Curceanu Catalina , Scordo Alessandro , Clozza Alberto , Davoli Ivan , Lucci Massimiliano , Francini Roberto , De Matteis Fabio , Grandi Maurizio , Tuladhar Rohisha , Grigolini Paolo
Published: April 2021

We study the emission of photons from germinating seeds using an experimental technique designed to detect light of extremely small intensity. We analyze the dark count signal without germinating seeds as well as the photon emission during the germination process. The technique of analysis adopted here, called diffusion entropy analysis (DEA) and originally designed to measure the temporal complexity of astrophysical, sociological and physiological processes, rests on Kolmogorov complexity. The updated version of DEA used in this paper is designed to determine if the signal complexity is generated either by non-ergodic crucial events with a non-stationary correlation function or by the infinite memory of a stationary but non-integrable correlation function or by a mixture of both processes. We find that dark count yields the ordinary scaling, thereby showing that no complexity of either kinds may occur without any seeds in the chamber. In the presence of seeds in the chamber anomalous scaling emerges, reminiscent of that found in neuro-physiological processes. However, this is a mixture of both processes and with the progress of germination the non-ergodic component tends to vanish and complexity becomes dominated by the stationary infinite memory. We illustrate some conjectures ranging from stress induced annihilation of crucial events to the emergence of quantum coherence.
Digitally Patterned Mesoporous Carbon Nanostructures of Colorless Polyimide for Transparent and Flexible Micro-Supercapacitor
Authors: Kim Hyeonwoo , Hwang Suwon , Hwang Taeseung , In Jung Bin , Yeo Junyeob
Published: April 2021

Here, we demonstrate the fabrication of a flexible and transparent micro-supercapacitor (MSC), using colorless polyimide (CPI) via a direct laser writing carbonization (DLWC) process. The focused laser beam directly carbonizes the CPI substrate and generates a porous carbon structure on the surface of the CPI substrate. Fluorine, which is one of the chemical compositions of CPI, can enhance the specific area and the conductivity of the carbon electrode by creating micropores in carbon structures during carbonization. Thus, the fabricated carbonized CPI-based MSC shows enhanced specific capacitance (1.20 mF at 10 mV s−1) and better transmittance (44.9%) compared to the conventional PI-based MSC. Additionally, the fabricated carbonized CPI-based MSC shows excellent cyclic performance with minimal reduction (<~10%) in 3000 cycles and high capacitance retention under mechanical bending test conditions. Due to its high flexibility, transparency, and capacitance, we expect that carbonized CPI-based MSC can be further applied to various flexible and transparent applications.
Immobilising Microalgae and Cyanobacteria as Biocomposites: New Opportunities to Intensify Algae Biotechnology and Bioprocessing
Authors: Caldwell Gary S. , In-na Pichaya , Hart Rachel , Sharp Elliot , Stefanova Assia , Pickersgill Matthew , Walker Matthew , Unthank Matthew , Perry Justin , Lee Jonathan G. M.
Published: April 2021

There is a groundswell of interest in applying phototrophic microorganisms, specifically microalgae and cyanobacteria, for biotechnology and ecosystem service applications. However, there are inherent challenges associated with conventional routes to their deployment (using ponds, raceways and photobioreactors) which are synonymous with suspension cultivation techniques. Cultivation as biofilms partly ameliorates these issues; however, based on the principles of process intensification, by taking a step beyond biofilms and exploiting nature inspired artificial cell immobilisation, new opportunities become available, particularly for applications requiring extensive deployment periods (e.g., carbon capture and wastewater bioremediation). We explore the rationale for, and approaches to immobilised cultivation, in particular the application of latex-based polymer immobilisation as living biocomposites. We discuss how biocomposites can be optimised at the design stage based on mass transfer limitations. Finally, we predict that biocomposites will have a defining role in realising the deployment of metabolically engineered organisms for real world applications that may tip the balance of risk towards their environmental deployment.
Numerical Investigation of High-Reynolds-Number Air-Ventilated Water Flow under Solid Body with Surface Geometry Variations
Authors: Matveev Konstantin I. , Collins Jeffrey M.
Published: April 2021

Air-ventilated cavities formed under or around the hulls of marine vehicles can reduce water drag. Hull configurations with partial air ventilation where air cavities reattach to body surfaces are of special practical interest, since the required air supply rates to achieve significant drag reduction can be made rather low. However, formation and stability of such air cavities are sensitive to the hull geometry and operational conditions. In this study, an attempt is made to numerically simulate one setup with a partial air cavity that was previously tested experimentally at high Reynolds numbers, above 50 million. A computational fluid dynamics software Star-CCM+ has been employed for numerical modeling. Stable and unstable states of the air-cavity setup, characterized by long and collapsing air cavities, respectively, were modeled at two air supply rates near the stability boundary. Numerical results were similar to experimental data at the optimal water speed for the tested geometry, when a long air cavity was sustained at a minimal air supply rate. For water speeds that were substantially higher or lower than the optimal case, a stable cavity could not be maintained with small air supply rates for the given hull geometry. Numerical simulations demonstrated how alterations of the body surface could help sustain long air cavities across a broader speed range using air supply rates that were similar to the optimal case. These findings suggest that morphing hull surfaces can potentially be used for control of drag-reducing air cavities and expand the viable operating range for their application to marine vehicles.
Geomechanical Behaviour of Uncemented Expanded Polystyrene (EPS) Beads–Clayey Soil Mixtures as Lightweight Fill
Authors: Abbasimaedeh Pouyan , Ghanbari Ali , O’Kelly Brendan C. , Tavanafar Mohsen , Irdmoosa Kourosh Ghaffari
Published: April 2021

Lightweight fill can be advantageous in embankment construction for the purposes of reducing the (i) bearing pressures on the underlying soil foundation, (ii) destabilizing moments for constructed earthen slopes, and (iii) earth pressures acting behind retaining walls. This paper investigates the merits/limitations of particulate expanded polystyrene (EPS) beads mixed with clayey sand (CS) soil as lightweight fill, considering both geotechnical and environmental perspectives. The bench-scale geotechnical testing programme included standard Proctor (SP) compaction, California bearing ratio (CBR), direct shear (sheardox), oedometer and permeability testing performed on two different gradation CS soils amended with 0.5, 1.5 and 3.0 wt.% EPS, investigating two nominal bead sizes equivalent to poorly-graded medium and coarse sands. Compared to the unamended soils, the compacted dry density substantially decreased with increasing EPS beads content, from 2.09 t/m3 (0 wt.% EPS) to as low as 0.33 t/m3 for 3 wt.% (73 v.%) of larger-sized EPS beads. However, from analyses of the test results for the investigated 50 to 400 kPa applied stress range, even 0.5 wt.% (21 v.%) EPS beads caused a substantial mechanical failure, with a drastic decay of the CBR and compressibility parameters for the studied CS soils. Given the more detrimental environmental cost of leaving myriads of separate EPS beads mixed forever among the soil, it is concluded that the approach of adding particulate EPS beads to soils for producing uncemented lightened fill should not be employed in geotechnical engineering practice.
The Effects of Monomer, Crosslinking Agent, and Filler Concentrations on the Viscoelastic and Swelling Properties of Poly(methacrylic acid) Hydrogels: A Comparison
Authors: Ninciuleanu Claudia Mihaela , Ianchiş Raluca , Alexandrescu Elvira , Mihăescu Cătălin Ionuţ , Scomoroşcenco Cristina , Nistor Cristina Lavinia , Preda Silviu , Petcu Cristian , Teodorescu Mircea
Published: April 2021

The present work aims at comparatively studying the effects of the concentrations of a monomer (10–30 wt% based on the whole hydrogel composition), crosslinking agent (1–3 mol% based on the monomer), and reinforcing agent (montmorillonite-MMT, 1–3 wt.% based on the whole hydrogel composition) on the swelling and viscoelastic properties of the crosslinked hydrogels prepared from methacrylic acid (MAA) and N,N′-methylenebisacrylamide (BIS) in the presence of K2S2O8 in aqueous solution. The viscoelastic measurements, carried out on the as-prepared hydrogels, showed that the monomer concentration had the largest impact, its three-time enhancement causing a 30-fold increase in the storage modulus, as compared with only a fivefold increase in the case of the crosslinking agent and 1.5-fold increase for MMT in response to a similar threefold concentration increase. Swelling studies, performed at three pH values, revealed that the water absorption of the hydrogels decreased with increasing concentration of both the monomer and crosslinking agent, with the amplitude of the effect of concentration modification being similar at pH 5.4 and 7.4 in both cases, but very different at pH 1.2. Further, it was shown that the increased pH differently influenced the swelling degree in the case of the hydrogel series in which the concentrations of the monomer and crosslinking agent were varied. In contrast to the effect of the monomer and crosslinking agent concentrations, the increase in the MMT amount in the hydrogel resulted in an increased swelling degree at pH 5.4 and 7.4, while at pH 1.2, a slight decrease in the water absorption was noticed. The hydrogel crosslinking density determinations revealed that this parameter was most affected by the increase in the monomer concentration.
Experimental Evaluation of Shear Behavior of Stone Masonry Wall
Authors: Beconcini Maria Luisa , Croce Pietro , Formichi Paolo , Landi Filippo , Puccini Benedetta
Published: April 2021

The evaluation of the shear behavior of masonry walls is a first fundamental step for the assessment of existing masonry structures in seismic zones. However, due to the complexity of modelling experimental behavior and the wide variety of masonry types characterizing historical structures, the definition of masonry’s mechanical behavior is still a critical issue. Since the possibility to perform in situ tests is very limited and often conflicting with the needs of preservation, the characterization of shear masonry behavior is generally based on reference values of mechanical properties provided in modern structural codes for recurrent masonry categories. In the paper, a combined test procedure for the experimental characterization of masonry mechanical parameters and the assessment of the shear behavior of masonry walls is presented together with the experimental results obtained on three stone masonry walls. The procedure consists of a combination of three different in situ tests to be performed on the investigated wall. First, a single flat jack test is executed to derive the normal compressive stress acting on the wall. Then a double flat jack test is carried out to estimate the elastic modulus. Finally, the proposed shear test is performed to derive the capacity curve and to estimate the shear modulus and the shear strength. The first results obtained in the experimental campaign carried out by the authors confirm the capability of the proposed methodology to assess the masonry mechanical parameters, reducing the uncertainty affecting the definition of capacity curves of walls and consequently the evaluation of seismic vulnerability of the investigated buildings.