bluuebunny/crossref_metadata_2025 huggingface dataset:
Interfacial Microstructure and Cladding Corrosion Resistance of Stainless Steel/Carbon Steel Clad Plates at Different Rolling Reduction Ratios
Authors: Chen Jie , Zhu Yixin , Chen Xia , Ma Xiaoli , Chen Bin
Published: December 2024

Optical microscope (OM), energy dispersive spectrometer (EDS), electron backscatter diffractometer (EBSD), electrochemical test, and transmission electron microscope (TEM) were employed to conduct interface microstructure observation and cladding corrosion resistance analysis on 304 SS/CS clad plates that have four different reduction ratios. The increase in rolling reduction ratio leads to larger grain size, gradually refined microstructure, and a decreased thickness of the interfacial martensite area. As the concentration disparity of the C element between carbon steel (CS) and 304 stainless steel (SS) is small, no evident carburization layer or decarburization layer can be detected. The ferrite microstructure on the CS side has greater stress distribution and greater local orientation deviation, and deformed grains are dominant. Austenite undergoes strain-induced martensitic transformation with the transformation mechanism of γ→twinning→a’-martensite. The martensite microstructure within the interface region grows in the direction of the interior of austenite grains. The reduction ratio increases sharply, leading to an increase in dislocation density, which promotes the nucleation, growth, and precipitation of carbides and seriously reduces the corrosion resistance of the cladding. Subsequently, the reduction ratio keeps on increasing. However, the degree of change in the reduction ratio diminishes. High temperature promotes the dissolution of carbides and improves the corrosion resistance. From this, it can be understood that by applying the process conditions of raising the reduction ratio and keeping a high temperature at the carbide dissolution temperature, a clad plate that has excellent interface bonding and remarkable corrosion resistance can be acquired.
Efficient Modeling Framework for FO-WLP Solder Interconnect Behavior During Thermal Cycling
Authors: Vargas Cruz Ramiro Sebastian , Gonda Viktor
Published: December 2024

In advanced microelectronic packaging, high thermo-mechanical loads arise on the solder interconnects. Accurate and efficient modeling of the mechanical behavior is crucial in the design of the package, and the simulation results can provide a basis for estimations of the reliability of the assembly. However, the accuracy of the simulation results depends on the accuracy of the modeled geometry and the modeling simplifications and assumptions employed to achieve computational cost-efficient calculations. In this work, finite element analysis (FEA) of a Fan Out—Wafer Level Packaging (FO-WLP) layout was carried out considering the following variations: modeling domain (2-D and pseudo-3-D) was defined for creating the efficient calculation framework, where soldering material (SAC 305 and SACQ), incorporation of intermetallic compound (IMC), bond pad edge geometry (sharp and blunt) were modeled for cycles of thermal load. Stress and strain analysis was carried out to evaluate the solder behavior for the parameter variations. Furthermore, fatigue indicators were evaluated. An efficient planar simulation framework with 2-D and pseudo-3-D meshed geometries provides a quick estimate for the lower and upper bound for the strain, stress and strain energy-related parameters, respectively. This calculation framework can be employed for extensive parameter studies solved rapidly at low computational costs.
Effects of Surface Modifications on Rotating Bending Fatigue of Ni-Al Bronze Alloy
Authors: Lu Guan-Xun , Chen Tai-Cheng , Shiue Ren-Kae , Tsay Leu-Wen
Published: December 2024

Surface modifications, including laser surface melting (LSM) and micro-shot peening (MSP), were applied to improve the fatigue performance of the Ni-Al bronze (NAB) alloy. LSM could homogenize the NAB into a uniform microstructure with refined columnar grains in the laser-melted zone but introduced residual tensile stress (RTS). The bombardment and generated heat induced by MSP could not entirely remove the granular κII precipitates and lamellar κIII phase in the peened zone of the shot-peened sample (NSP sample) but introduced residual compressive stress (RCS) into the NSP sample under the peening intensity of this work. The results of fatigue tests revealed that the LSM sample had the poorest fatigue performance, but the NSP sample showed the best fatigue performance among the tested samples. The fatigue limit of the NAB alloy was about 325 MPa; meanwhile, the fatigue performance of the LSP (LSM + MSP) sample was equivalent to or a little better than that of the NAB alloy (NBM sample). The RTS and aligned columnar grains accounted for the degraded fatigue resistance of the LSM sample. By contrast, the high RCS and the refined structure were responsible for the improved fatigue strength/life of the NSP sample relative to that of the other samples. The fatigue limit of the NSP sample was as high as 450 MPa. However, the increase in fatigue strength of the NSP sample occurred in service life regions above 3 × 105 cycles.
Wettability of Polar and Apolar Liquids on Metal Surfaces
Authors: Khairi Mohanad , Erdélyi Zoltán , Baumli Peter
Published: December 2024

The wettability of metal surfaces by different oils and water is a multifaceted phenomenon with significant implications for industrial processes, including lubrication, corrosion protection, and fluid transport; an understanding of the process is essential for optimizing the performance and durability of metallic components. The intermolecular interactions between oil molecules and the metal surface primarily influence the wetting of a metal surface by different types of oil. This paper introduces the concept of oil wetting on metal surfaces, exploring the factors influencing wetting behavior, the characterization techniques employed to assess wetting properties, and the implications for different industrial processes. This work aims to ascertain the contact angle of oil on various metal surfaces and subsequently establish a relationship between this contact angle and the attributes of the substrate. This is achieved through using the sessile drop technique. The results indicate that the wettability of petroleum was better than the hydraulic oil we used on all types of substrates (for example, on Ag surface, Θ-petroleum = 11°, but Θ-hydraulic oil = 20°). Also, we observed that the cosine of the oil/metal contact angle increases with the increase in the atomic radius of the pure metal substrate, and Becker’s broken bond model proved this linear relation. We then contrast this behavior with the wetting characteristics of water and glycerin on the same metals using the same conditions.
Additive Manufacturing of Smart Footwear Components for Healthcare Applications
Authors: Kundumani Janarthanan Aravind Kanna , Vaidhyanathan Bala
Published: December 2024

Diabetic foot complications pose significant health risks, necessitating innovative approaches in orthotic design. This study explores the potential of additive manufacturing in producing functional footwear components with lattice-based structures for diabetic foot orthoses. Five distinct lattice structures (gyroid, diamond, Schwarz P, Split P, and honeycomb) were designed and fabricated using stereolithography (SLA) with varying strand thicknesses and resin types. Mechanical testing revealed that the Schwarz P lattice exhibited superior compressive strength, particularly when fabricated with flexible resin. Porosity analysis demonstrated significant variations across structures, with the gyroid showing the most pronounced changes with increasing mesh thickness. Real-time pressure distribution mapping, achieved through integrated force-sensitive resistors and Arduino-based data acquisition, enabled the visualization of pressure hotspots across the insole. The correlation between lattice properties and pressure distribution was established, allowing for tailored designs that effectively alleviated high-pressure areas. This study demonstrates the feasibility of creating highly personalized orthotic solutions for diabetic patients using additive manufacturing, offering a promising approach to reducing the plantar pressure in foot and may contribute to improved outcomes in diabetic foot care.
Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane
Authors: Jiang Nianyu , Shrotriya Pranav
Published: December 2024

We report a low-cost, portable biosensor composed of an aptamer-functionalized nanoporous anodic aluminum oxide (NAAO) membrane and a commercial microcontroller chip-based impedance reader suitable for electrochemical impedance spectroscopy (EIS)-based sensing. The biosensor consists of two chambers separated by an aptamer-functionalized NAAO membrane, and the impedance reader is utilized to monitor transmembrane impedance changes. The biosensor is utilized to detect amodiaquine molecules using an amodiaquine-binding aptamer (OR7)-functionalized membrane. The aptamer-functionalized membrane is exposed to different concentrations of amodiaquine molecules to characterize the sensitivity of the sensor response. The specificity of the sensor response is characterized by exposure to varying concentrations of chloroquine, which is similar in structure to amodiaquine but does not bind to the OR7 aptamer. A commercial potentiostat is also used to measure the sensor response for amodiaquine and chloroquine. The sensing response measured using both the portable impedance reader and the commercial potentiostat showed a similar dynamic response and detection threshold. The specific and sensitive sensing results for amodiaquine demonstrate the efficacy of the low-cost and portable biosensor.
MEMS Acoustic Sensors: Charting the Path from Research to Real-World Applications
Authors: Wang Qingyi , Zhang Yang , Cheng Sizhe , Wang Xianyang , Wu Shengjun , Liu Xufeng
Published: December 2024

MEMS acoustic sensors are a type of physical quantity sensor based on MEMS manufacturing technology for detecting sound waves. They utilize various sensitive structures such as thin films, cantilever beams, or cilia to collect acoustic energy, and use certain transduction principles to read out the generated strain, thereby obtaining the targeted acoustic signal’s information, such as its intensity, direction, and distribution. Due to their advantages in miniaturization, low power consumption, high precision, high consistency, high repeatability, high reliability, and ease of integration, MEMS acoustic sensors are widely applied in many areas, such as consumer electronics, industrial perception, military equipment, and health monitoring. Through different sensing mechanisms, they can be used to detect sound energy density, acoustic pressure distribution, and sound wave direction. This article focuses on piezoelectric, piezoresistive, capacitive, and optical MEMS acoustic sensors, showcasing their development in recent years, as well as innovations in their structure, process, and design methods. Then, this review compares the performance of devices with similar working principles. MEMS acoustic sensors have been increasingly widely applied in various fields, including traditional advantage areas such as microphones, stethoscopes, hydrophones, and ultrasound imaging, and cutting-edge fields such as biomedical wearable and implantable devices.
A Compact Broadband Common-Aperture Dual-Polarized Antenna for Drone Applications
Authors: Li Xue-Ping , He Chao-Liang , Ji Jun-Fei , Yang Meng-Bing , Zhang Yan , Zhang An-Xue , Li Wei
Published: December 2024

A novel common-aperture miniaturized antenna with wideband and dual-polarized characteristics is proposed, which consists of a circularly polarized (CP) and a linearly polarized (LP) antenna. The circularly polarized antenna stacked on the upper layer adopts asymmetrical ground and introduces the patch and T-type feed network. On this basis, the meshed reflector structure, which also works as a ground plane for the LP antenna, is added to reduce the influence on circular polarization and achieve directional radiation. The LP antenna stacked in the lower layer uses a monopole structure, and the coaxial feed line perforates the reflector, and thereby the common-aperture antennas are tightly stacked together from top to bottom. Simulation and test are in good accordance, and the results show that the two ports of the antenna are well matched in the range of 5.5 GHz to 7.8 GHz, where peak gains of 8.5 dB and 6 dB are realized for circular polarization and linear polarization, respectively. Moreover, the 3 dB axial ratio (AR) bandwidth of the CP antenna is 34.3% and the isolation between the two ports is better than 15 dB, suggesting potential applications in the relay platform or drone detection for signal transmission and reception.
Optimization of Flexible Rotor for Ultrasonic Motor Based on Response Surface and Genetic Algorithm
Authors: Chen Bo , Yang Jiyue , Tang Haoyu , Wu Yahang , Zhang Haoran
Published: December 2024

The flexible rotor, as a crucial component of the traveling wave rotary ultrasonic motor, effectively reduces radial friction. However, issues such as uneven contact between the stator and rotor, as well as rotor-deformation-induced stress, still persist. This paper presents an optimization method that combines the Kriging response surface model with a multi-objective genetic algorithm (MOGA). Drawing on the existing rotor structure, a novel rotor design is proposed to match the improved TRUM60 stator. During the optimization process, the contact surface between the stator and rotor is taken as the optimization target, and an objective function is established. The Kriging response surface model is constructed using Latin hypercube sampling, and an MOGA is employed to optimize this model, allowing the selection of the optimal balanced solution from multiple candidate designs. Following stator optimization, the objective function value decreased from 0.631 to 0.036, and the maximum contact stress on the rotor inner ring was reduced from 32.77 MPa to 9.96 MPa. Experimental validation confirmed the reliability of this design, significantly improving the overall performance and durability of the motor.
SiC MOSFET with Integrated SBD Device Performance Prediction Method Based on Neural Network
Authors: Niu Xiping , Sang Ling , Duan Xiaoling , Gu Shijie , Zhao Peng , Zhu Tao , Xu Kaixuan , He Yawei , Li Zheyang , Zhang Jincheng , Jin Rui
Published: December 2024

The SiC MOSFET with an integrated SBD (SBD-MOSFET) exhibits excellent performance in power electronics. However, the static and dynamic characteristics of this device are influenced by a multitude of parameters, and traditional TCAD simulation methods are often characterized by their complexity. Due to the increasing research on neural networks in recent years, such as the application of neural networks to the prediction of GaN JBS and Finfet devices, this paper considers the application of neural networks to the performance prediction of SiC MOSFET devices with an integrated SBD. This study introduces a novel approach utilizing neural network machine learning to predict the static and dynamic characteristics of the SBD-MOSFET. In this research, SBD-MOSFET devices are modeled and simulated using Sentaurus TCAD(2017) software, resulting in the generation of 625 sets of device structure and sample data, which serve as the sample set for the neural network. These input variables are then fed into the neural network for prediction. The findings indicate that the mean square error (MSE) values for the threshold voltage (Vth), breakdown voltage (BV), specific on-resistance (Ron), and total switching power dissipation (E) are 0.0051, 0.0031, 0.0065, and 0.0220, respectively, demonstrating a high degree of accuracy in the predicted values. Meanwhile, in the comparison of convolutional neural networks and machine learning, the CNN accuracy is much higher than the machine learning methods. This method of predicting device performance via neural networks offers a rapid means of designing SBD-MOSFETs with specified performance targets, thereby presenting significant advantages in accelerating research on SBD-MOSFET performance prediction.
Fabrication and Electrical Characterization of Low-Temperature Polysilicon Films for Sensor Applications
Authors: Mota Filipa C. , Garcia Inês S. , Retolaza Aritz , Santos Dimitri E. , Sousa Patrícia C. , Aguiam Diogo E. , Dias Rosana A. , Calaza Carlos , Silva Alexandre F. , Alves Filipe S.
Published: December 2024

The development of low-temperature piezoresistive materials provides compatibility with standard silicon-based MEMS fabrication processes. Additionally, it enables the use of such material in flexible substrates, thereby expanding the potential for various device applications. This work demonstrates, for the first time, the fabrication of a 200 nm polycrystalline silicon thin film through a metal-induced crystallization process mediated by an AlSiCu alloy at temperatures as low as 450 °C on top of silicon and polyimide (PI) substrates. The resulting polycrystalline film structure exhibits crystallites with a size of approximately 58 nm, forming polysilicon (poly-Si) grains with diameters between 1–3 µm for Si substrates and 3–7 µm for flexible PI substrates. The mechanical and electrical properties of the poly-Si were experimentally conducted using microfabricated test structures containing piezoresistors formed by poly-Si with different dimensions. The poly-Si material reveals a longitudinal gauge factor (GF) of 12.31 and a transversal GF of −4.90, evaluated using a four-point bending setup. Additionally, the material has a linear temperature coefficient of resistance (TCR) of −2471 ppm/°C. These results illustrate the potential of using this low-temperature film for pressure, force, or temperature sensors. The developed film also demonstrated sensitivity to light, indicating that the developed material can also be explored in photo-sensitive applications.
Tunable Filters Using Defected Ground Structures at Millimeter-Wave Frequencies
Authors: Annam Kaushik , Alemayehu Birhanu , Shin Eunsung , Subramanyam Guru
Published: December 2024

This paper explores the potential of phase change materials (PCM) for dynamically tuning the frequency response of a dumbbell u-slot defected ground structure (DGS)-based band stop filter. The DGSs are designed using co-planar waveguide (CPW) line structure on top of a barium strontium titanate (Ba0.6Sr0.4TiO3) (BST) thin film. BST film is used as the high-dielectric material for the planar DGS. Lower insertion loss of less than −2 dB below the lower cutoff frequency, and enhanced band-rejection with notch depth of −39.64 dB at 27.75 GHz is achieved by cascading two-unit cells, compared to −12.26 dB rejection with a single-unit cell using BST thin film only. Further tunability is achieved by using a germanium telluride (GeTe) PCM layer. The electrical properties of PCM can be reversibly altered by transitioning between amorphous and crystalline phases. We demonstrate that incorporating a PCM layer into a DGS device allows for significant tuning of the resonance frequency: a shift in resonance frequency from 30.75 GHz to 33 GHz with a frequency shift of 2.25 GHz is achieved, i.e., 7.32% tuning is shown with a single DGS cell. Furthermore, by cascading two DGS cells with PCM, an even wider tuning range is achievable: a shift in resonance frequency from 27 GHz to 30.25 GHz with a frequency shift of 3.25 GHz is achieved, i.e., 12.04% tuning is shown by cascading two DGS cells. The results are validated through simulations and measurements, showcasing excellent agreement.
Molecular Characterization and Pathogenicity of Colletotrichum on Banana Fruits: Wound Effects on Virulence and Cross-Infection
Authors: Santos Maysa C. , Viteri Luis O. , Araujo Sabrina H. , Mourão Dalmarcia C. , Câmara Marcos P. , Amaral Ana G. , Oliveira Eugênio E. , Santos Gil Rodrigues dos
Published: December 2024

For this article, we evaluated whether wounds would affect the pathogenicity and virulence of Colletotrichum sp. isolates on Musa spp. banana cultivars. We further assessed the potential of cross-colonization with other fruit species and investigated the molecular and phylogenetic characterization of the most virulent isolates. Firstly, we collected dwarf bananas showing anthracnose symptoms from commercial markets in the city of Gurupi, Tocantins State, Brazil, and isolated Colletotrichum sp. under controlled conditions prior to identification. The virulence was assessed on wounded and unwounded banana fruits, identifying the most virulent isolate by exposure tests on fruits of the “prata”, “maçã”, “marmelo”, and “terra” banana cultivars. We also subjected specimens of mango (Mangifera indica), papaya (Carica papaya), and apple (Malus domestica) fruits to the exposure tests. Our results indicated that pathogenicity varies with the isolate (with C2, C8, and C10 as the most virulent), fruit condition (wounded fruits are the most susceptible), and cultivars (terra, marmela, and maça are the most susceptible). All isolates were more virulent on wounded bananas, while those on unwounded ones showed lower virulence. Among the banana cultivars, “prata” fruits were the most susceptible, regardless of wounding. Additionally, Colletotrichum isolates from dwarf bananas were pathogenic to mango, papaya, and apple fruits. Furthermore, our results demonstrated that the most virulent isolates belong to the species C. musae. Collectively, our findings reinforce the relevance of minimizing post-harvest wounds on banana fruits and highlight the risks of cross-infection when storing bananas alongside other fruit species.
Cooperation Between Rhodococcus qinshengii and Rhodococcus erythropolis for Carbendazim Degradation
Authors: Solano-Rodríguez Roosivelt , Santoyo-Tepole Fortunata , Figueroa Mario , Larios-Serrato Voleta , Ruiz-Ordaz Nora , Pérez-Valdespino Abigail , Curiel-Quesada Everardo
Published: December 2024

Carbendazim (CBZ) is a fungicide widely used on different crops, including soybeans, cereals, cotton, tobacco, peanuts, and sugar beet. Excessive use of this xenobiotic causes environmental deterioration and affects human health. Microbial metabolism is one of the most efficient ways of carbendazim elimination. In this work, Rhodococcus qingshengii RC1 and Rhodococcus erythropolis RC9 were isolated from a bacterial community growing in a biofilm reactor acclimated with microbiota from carbendazim-contaminated soil. Sequencing analysis of genomes of both strains revealed the presence of cbmA, the gene coding for the enzyme that hydrolyses carbendazim to produce 2-aminobenzimidazole (2-AB). The alternative gene for the first catabolic step (mheI) was detected by PCR in strain RC9 but not in RC1. Metabolomic analysis by HPLC and LC-MS showed that both strains have the ability to metabolize carbendazim. R. qingshengii RC1 converts carbendazim to 2-AB, the first degradation intermediary, while R. erythropolis RC9 metabolizes the fungicide to its mineralization, probably because R. qingshengii RC1 lacks the hdx gene coding for 2-AB hydroxylase. HRESIMS-MS/MS results indicate that R. erythropolis RC9 metabolizes carbendazim by cleavage of the benzene ring and subsequent formation of 5-formyl-2-hydroxy-4,5-dihydro-1H-imidazole-4-carboxylic acid (X2 C5H6N2O4). The presence of carbendazim metabolites in culture supernatants of strains RC9 and RC1 suggests that both strains contribute to the efficient degradation of carbendazim in nature.
Exploring the Potential of Candida sp. SW4-6 as a Probiotic for Enhancing Water Quality in Aquaculture
Authors: Li Jie-Ying , Liu Chun-Hung
Published: December 2024

Aquaculture, a vital industry supplying a significant portion of the world’s seafood, faces challenges such as the deterioration of the aquaculture environment. The objective of this study was to isolate and identify microorganisms with the capacity to eliminate nitrite in water from shrimp ponds and evaluate their potential as probiotics to improve water quality. Additionally, the study also determines the ideal conditions for the probiotic to effectively reduce nitrite-N and ammonia-N. Water samples were collected from four shrimp ponds (SW1, SW2, SW3, SW4) and isolates were obtained. Among all the samples, SW4 was the most effective in reducing the concentration of nitrite-N. Upon further isolation of SW4, the strain SW4-W6 showed significant nitrite-N reduction capabilities compared to the 19 other isolates tested. Through morphological, genetic (ITS sequence), and phylogenetic analyses, strain SW4-6 was identified as Candida sp. The isolation of Candida sp. SW4-6 showed superior nitrite-N and ammonia-N reduction capabilities, with sucrose as the carbon source and complete reduction observed at a C/N ratio of 15–20. Gene expression analysis revealed the up-regulation of nitrite reductase in SW4-6 after inoculation, with significantly higher expression observed with sucrose as the carbon source. Salinity and temperature significantly influenced nitrite-N and ammonia-N reduction by SW4-6, with higher temperatures (30 °C) and 0% NaCl favoring faster reduction rates. Candida sp. SW4-6 emerges as a promising probiotic candidate for aquaculture water quality management due to its efficient nitrite-N and ammonia-N reduction capabilities under optimal conditions. Its virulence profile and ability to thrive across various salinity and temperature conditions further support its potential applicability in aquaculture.
A Rewired NADPH-Dependent Redox Shuttle for Testing Peroxisomal Compartmentalization of Synthetic Metabolic Pathways in Komagataella phaffii
Authors: Fina Albert , Àvila-Cabré Sílvia , Vázquez-Pereira Enrique , Albiol Joan , Ferrer Pau
Published: December 2024

The introduction of heterologous pathways into microbial cell compartments offers several potential advantages, including increasing enzyme concentrations and reducing competition with native pathways, making this approach attractive for producing complex metabolites like fatty acids and fatty alcohols. However, measuring subcellular concentrations of these metabolites remains technically challenging. Here, we explored 3-hydroxypropionic acid (3-HP), readily quantifiable and sharing the same precursors—acetyl-CoA, NADPH, and ATP—with the above-mentioned products, as a reporter metabolite for peroxisomal engineering in the yeast Komagataella phaffii. To this end, the malonyl-CoA reductase pathway for 3-HP production was targeted into the peroxisome of K. phaffii using the PTS1-tagging system, and further tested with different carbon sources. Thereafter, we used compartmentalized 3-HP production as a reporter system to showcase the impact of different strategies aimed at enhancing the peroxisomal NADPH pool. Co-overexpression of genes encoding a NADPH-dependent redox shuttle from Saccharomyces cerevisiae (IDP2/IDP3) significantly increased 3-HP yields across all substrates, whereas peroxisomal targeting of the S. cerevisiae NADH kinase Pos5 failed to improve 3-HP production. This study highlights the potential of using peroxisomal 3-HP production as a biosensor for evaluating peroxisomal acetyl-CoA and NAPDH availability by simply quantifying 3-HP, demonstrating its potential for peroxisome-based metabolic engineering in yeast.
Invasion of Chicken Intestinal Cells Is Higher for Enterococcus cecorum Lesion Strains Compared to Cloacal Strains in an Organoid Model
Authors: Vervelde Lonneke , Manders Thijs T. M. , Kammourieh Samira , Wiegel Jeanine
Published: December 2024

Some strains of Enterococcus cecorum can cause spondylitis and bacterial osteomyelitis. Translocation and bacteremia are pivotal to the pathogenesis and clinical disease. Virulence typing to distinguish extra-intestinal disease of lesion from cloacal strains remains difficult. We investigated if organoids can be applied to differentiate between E. cecorum strains that are more or less virulent. Floating chicken intestinal organoids combine the complex cell system of the gut with an easily accessible apical-out orientation. The organoids were treated with four E. cecorum strains that differ in original isolation, lesion, or cloacal, and bacterial load was determined after 3 and 6 h by quantitative PCR and bacterial plating. Independent of the inoculum dose or time post inoculation, DNA levels of E. cecorum marginally differed between the strains. To determine if this was caused by adherence of bacteria to the epithelial cells, an invasion assay was developed. The organoids were inoculated with the different E. cecorum strains and after 3 or 6 h treated with an antimicrobial mixture, lysed, and quantified by bacterial plate counting. Significantly higher (p < 0.0001) numbers of bacteria isolated from lesions invaded the organoids compared to cloacal strains in a dose-dependent manner. Higher numbers of bacteria isolated from lesions invaded the organoids compared to cloacal strains in a dose-dependent manner. This study is a major step in the development of a model to study the interaction between E. cecorum and the chicken host and a model to test novel intervention strategies to prevent translocation of bacteria.
Recent Advances in Antimicrobial Resistance: Insights from Escherichia coli as a Model Organism
Authors: Zhang Zhaoyang , Wei Minliang , Jia Bin , Yuan Yingjin
Published: December 2024

Antimicrobial resistance (AMR) represents a critical global health threat, and a thorough understanding of resistance mechanisms in Escherichia coli is needed to guide effective treatment interventions. This review explores recent advances for investigating AMR in E. coli, including machine learning for resistance pattern analysis, laboratory evolution to generate resistant mutants, mutant library construction, and genome sequencing for in-depth characterization. Key resistance mechanisms are discussed, including drug inactivation, target modification, altered transport, and metabolic adaptation. Additionally, we highlight strategies to mitigate the spread of AMR, such as dynamic resistance monitoring, innovative therapies like phage therapy and CRISPR-Cas technology, and tighter regulation of antibiotic use in animal production systems. This review provides actionable insights into E. coli resistance mechanisms and identifies promising directions for future antibiotic development and AMR management.
Prospective Investigation of Nanoplastic Accumulation in Healthy Subjects, Autoimmune Diseases, Hematological Malignancies, Lung Cancer, and Murine Models
Authors: Salvia Roser , Cañaveras Manel , Rico Laura G. , Drozdowskyj Ana , Ward Michael D. , Jurado Rebeca , Gómez-Muñoz Laia , Vives-Pi Marta , Martínez-Cáceres Eva , Petriz Jordi
Published: December 2024

Nanoplastics (NPs) and microplastics (MPs) are an emerging threat to global health. They negatively impact ecosystems and many physiological processes, causing alterations in xenobiotic metabolism, nutrient uptake, energy metabolism, or cytotoxicity. In humans, we are beginning to analyze these plastics for the mechanisms by which they enter the organism, accumulate, and diffuse as well as for their pathogenic potential. NP accumulation has been demonstrated in human tissues, such as blood or placenta, while in others it remains largely unstudied. In this work, we detected NP accumulation in bronchoalveolar lavage fluids (BALFs), cerebrospinal fluids (CSFs), lymph nodes (LNs), urine, pleural fluids (PFs), ascitic fluids (AFs), and peripheral blood (PB) by combining fluorescence and nanocytometry techniques. NP analysis was compared with two strains of mice, and the results support that inhalation is the main route of NP accumulation.
Integrating Magnetic and Self-Potential Methods for Efficient Graphite Exploration: Insights from Ji’an, Northeast China
Authors: Liu Junchen , Yi Jiwei , Li Chuncheng , Gao Ci
Published: December 2024

Graphite, known for its exceptional electrical and thermal conductivity as well as its lubricating properties, is a critical mineral resource for various industrial applications. Due to these unique properties, graphite has gained increasing importance across various technological and industrial fields. Northeast China, rich in graphite deposits, faces significant exploration challenges owing to dense vegetation and limited bedrock exposure. This study provides a comprehensive analysis of the geological strata in the region, utilizing magnetic exploration to identify ore-forming strata beneath the Quaternary sediment. Additionally, self-potential methods were used to delineate areas of potential graphite mineralization. The effectiveness of these methods was evaluated across two well-tested and thoroughly studied geological profiles. The magnetic anomaly revealed three magnetic anomaly areas, with the low magnetic anomaly zone associated the Huangchagou Formation, indicating strong potential for graphite ore. Then, a self-potential survey was conducted across this anomalous zone, revealing a nearly east–west trending banded anomaly, suggesting the presence of graphite deposits. Trenching and drilling operations were subsequently carried out, confirming the existence of graphite ore, with total reserves exceeding 50,000 kt. This research demonstrates that the combination of magnetic surveys and self-potential methods offers a cost-effective geophysical approach for graphite exploration. These methods provide a promising strategy for discovering graphite deposits, particularly in the challenging terrains of Northeast China.
On the Question of Finding Relationship Between Structural Features of Smectites and Adsorption and Surface Properties of Bentonites
Authors: Krupskaya Victoria , Zakusin Sergey , Zakusina Olga , Belousov Petr , Pokidko Boris , Morozov Ivan , Zaitseva Tatiana , Tyupina Ekaterina , Koroleva Tatiana
Published: December 2024

During the development of the disposal concept in a crystalline massif of the Russian Federation, a question arose regarding the selection of the most suitable types of bentonite for a buffer layer. Data on the composition, structure, surface, and adsorption properties of four promising deposits have been obtained. The correlations between genesis and specific surface area (SSA) revealed in this study indicate that bentonites of volcanogenic-sedimentary origin have lower SSA in comparison with those of sedimentary and hydrothermal origin. The main contribution to the charge of the 2:1 layer is made by isomorphic substitutions in the tetrahedral sheet, which directly affects the sorption properties of bentonites. The influence of smectite structure on adsorption properties have been described. In particular, the dependencies between the layer charge and cation exchange capacity (CEC) have been analyzed. At the same time, the research indicates that, unlike the CEC, the specific surface area of a deposit is determined by its geological history. Special attention is being paid to refining methodological approaches for deriving crystal chemical formulae of smectites, which will be needed in the future for modelling changes in bentonite over a long-term perspective.
Gravity Survey for Mineral Exploration in Gerolekas Bauxite Mining Site in Greece
Authors: Karaiskos Dimitrios , Apostolopoulos Georgios , Orfanos Christos
Published: December 2024

This study presents a gravity survey conducted for mineral exploration in the Gerolekas overthrust area at a bauxite mining site in Central Greece. In the summer of 2018, a gravity survey, covering 28 km2, was conducted, including confirmed and unexplored zones. By utilizing gravity data, we investigated the shallow subsurface geology and structural sequences, resulting in a high-resolution 3D density model. This model is generated through constrained gravity inversion by the exploitation of the boreholes available at mining sites, and the geological survey fills the areas with boreholes, which provides the stratigraphy to some depth. The suggested data-processing techniques provide information for the tectonism of the area, which is also important for mineral exploration, as well as mining design. The interface with density contrast between the flysch and the underlying limestone in the high-resolution 3D density model provides useful information on the geological status, but also, the slight density difference in limestone provides an interface where bauxite deposits can be. The inversion, conducted with EMIGMA software, incorporated high-resolution topography data and density constraints to produce a reliable 3D density model. The findings highlight the gravity method’s potential to enhance mineral exploration efficiency, offering a robust tool for further geological and mining considerations.
Synthetic Approaches Toward Phosphorus-Containing BODIPY and Squaraine Dyes: Enhancing Versatility of Small-Molecule Fluorophores
Authors: Favret Jeanne M. , Dzyuba Sergei V.
Published: December 2024

Phosphorus-containing fluorophores provide a versatile framework for tailoring photophysical properties, enabling the design of advanced fluorogenic materials for various applications. Boron dipyrromethene (BODIPY) and squaraine dyes are of interest due to their multifaceted modularity and synthetic accessibility. Incorporating phosphorus-based functional groups into BODIPY or squaraine scaffolds has been achieved through a plethora of synthetic methods, including post-dye assembly functionalization. These modifications often influence key spectroscopic properties and molecular functionality by expanding their utility in bioimaging, sensing, photosensitization, and theranostic applications. By leveraging the tunable nature of phosphorus-containing moieties, these dyes hold immense promise for addressing current challenges in spectroscopy, imaging, and material designs while unlocking new opportunities for advanced functional systems in chemistry, biology, and medicine.
Iron Oxide Nanoparticle-Based T1 Contrast Agents for Magnetic Resonance Imaging: A Review
Authors: Zhang Dongmei , Zhang Jing , Bian Xianglin , Zhang Pei , Wu Weihua , Zuo Xudong
Published: December 2024

This review highlights recent progress in utilizing iron oxide nanoparticles (IONPs) as a safer alternative to gadolinium-based contrast agents (GBCAs) for magnetic resonance imaging (MRI). It consolidates findings from multiple studies, discussing current T1 contrast agents (CAs), the synthesis techniques for IONPs, the theoretical principles for designing IONP-based MRI CAs, and the key factors that impact their T1 contrast efficacy, such as nanoparticle size, morphology, surface modifications, valence states, and oxygen vacancies. Furthermore, we summarize current strategies to achieve IONP-based responsive CAs, including self-assembly/disassembly and distance adjustment. This review also evaluates the biocompatibility, organ accumulation, and clearance pathways of IONPs for clinical applications. Finally, the challenges associated with the clinical translation of IONP-based T1 CAs are included.
Flat-Band AC Transport in Nanowires
Authors: Sánchez Vicenta , Wang Chumin
Published: December 2024

The electronic states in flat bands possess zero group velocity and null charge mobility. Recently, flat electronic bands with fully localized states have been predicted in nanowires, when their hopping integrals between first, second, and third neighbors satisfy determined relationships. Experimentally, these relationships can only be closely achieved under external pressures. In this article, we study the alternating current (AC) in such nanowires having nearly flat electronic bands by means of a new independent channel method developed for the Kubo–Greenwood formula including hopping integrals up to third neighbors. The results reveal a large AC conductivity sensitive to the boundary conditions of measurement, where the charge carriers resonate with the external electric field by oscillating around their localized positions.
A Hierarchical Core-Shell Structure of NiO@Cu2O-CF for Effective Non-Enzymatic Electrochemical Glucose Detection
Authors: Huang Yueyun , You Jiahua , Ding Yingru , Xie Yun , Wang Ting , Zhu Fanglong , Gong Weiping , Zhao Zhenting
Published: December 2024

Non-enzymatic glucose detection is an effective strategy to control the blood glucose level of diabetic patients. A novel hierarchical core–shell structure of nickel hydroxide shell coated copper hydroxide core based on copper foam (Ni(OH)2@Cu(OH)2-CF) was fabricated and derived from NiO@Cu2O-CF for glucose sensing. Cyclic voltammetry and amperometry experiments have demonstrated the efficient electrochemical catalysis of glucose under alkaline conditions. The measurement displays that the fabricated sensor exhibits a detection scale of 0.005–4.5 mM with a detection sensitivity of 4.67 µA/µM/cm2. It has remarkable response/recovery times in respect of 750 μM glucose (1.0 s/3.5 s). Moreover, the NiO@Cu2O-CF shows significant selectivity, reliable reproducibility and long-term stability for glucose determination, suggesting it is a suitable candidate for further applications.
Pediatric Acute Disseminated Encephalomyelitis Triggered by Concurrent Administration of Seasonal and H1N1 Influenza Vaccines: A Case Report and Review
Authors: Imataka George , Shiraishi Hideaki , Yoshihara Shigemi
Published: December 2024

Background: Acute disseminated encephalomyelitis (ADEM) is a rare, immune-mediated inflammatory disorder of the central nervous system (CNS), typically characterized by the acute onset of multifocal demyelination. The pathogenesis of ADEM remains unclear, but it is believed to be triggered by an autoimmune response, often following viral infections or vaccinations. Case report: This case report describes a 3-year-old child who developed ADEM after receiving two concurrent influenza vaccines: one for seasonal influenza and one for the 2009 H1N1 pandemic. The patient presented with motor regression, mild pleocytosis in cerebrospinal fluid (CSF), and typical MRI findings of ADEM. Steroid pulse therapy resulted in rapid improvement, and the patient recovered fully without sequelae. Results: Although the influenza vaccine has been linked to ADEM in some studies, it remains uncertain whether the simultaneous administration of both vaccines contributed to the onset of ADEM. While influenza vaccines are considered safe and effective by health organizations such as the CDC, data suggest that the incidence of ADEM and other neurological complications is significantly higher after natural influenza infections compared to vaccination. This highlights the importance of vaccination in preventing severe outcomes. Conclusions: This case underscores the importance of monitoring and reporting adverse events following vaccination to refine our understanding of rare complications like ADEM. While simultaneous vaccine administration warrants further research, the benefits of vaccination in preventing severe complications from natural infections far outweigh the risks. Continued vigilance and improved surveillance systems are essential for maintaining public confidence in vaccination programs.
The Positive Effects of Training and Time-Restricted Eating in Gut Microbiota Biodiversity in Patients with Multiple Myeloma
Authors: Czerwińska-Ledwig Olga , Nowak-Zaleska Alicja , Żychowska Małgorzata , Meyza Katarzyna , Pałka Tomasz , Dzidek Adrianna , Szlachetka Agata , Jurczyszyn Artur , Piotrowska Anna
Published: December 2024

Background: The physical activity of different groups of individuals results in the rearrangement of microbiota composition toward a symbiotic microbiota profile. This applies to both healthy and diseased individuals. Multiple myeloma (MM), one of the more common hematological malignancies, predominantly affects older adults. Identifying an appropriate form of physical activity for this patient group remains a challenge. The aim of this study was to investigate the impact of a 6-week Nordic walking (NW) training program combined with a 10/14 time-restricted eating regimen on the gut microbiota composition of multiple myeloma patients. Methods: This study included healthy individuals as the control group (n = 16; mean age: 62.19 ± 5.4) and patients with multiple myeloma in remission (MM group; n = 16; mean age: 65.00 ± 5.13; mean disease duration: 57 months). The training intervention was applied to the patient group and consisted of three moderate-intensity sessions per week, individually tailored to the estimated physical capacity of each participant. The taxonomic composition was determined via 16S rRNA sequencing (V3–V9 regions). The microbiota composition was compared between the patient group and the control group. Results: The alpha and beta diversity metrics for species and genus levels differed significantly between the control and patient groups before the implementation of the NW program. In contrast, no differences were observed between the control and patient groups after the training cycle, indicating that the patients’ microbiota changed toward the pattern of the control group. This is confirmed by the lowest values of average dissimilarity between the MMB groups and the control at all taxonomic levels, as well as the highest one between the control group and the MMA patient group. The gut microbiota of the patients was predominantly represented by the phyla Firmicutes, Actinobacteria, Verrucomicrobia, Proteobacteria, and Bacteroidetes. Conclusions: The training, combined with time-restricted eating, stimulated an increase in the biodiversity and taxonomic rearrangement of the gut microbiota species.
The Contribution of Precision Nutrition Intervention in Subfertile Couples
Authors: Monteiro Jéssica , Bicho Manuel , Valente Ana
Published: December 2024

Background/Aim: Subfertility is characterized by a decrease in reproductive efficiency, which can result in delayed pregnancy, and affects one in six individuals during their lifetime. The present narrative review aims to evaluate the contribution of precision nutrition to changes in fertility in subfertile couples. Methods: The literature review was carried out through bibliographic research in the PubMed, Scopus, SciELO and Google Scholar databases. The following search criteria were applied: (1) original articles and narrative, systematic or meta-analytic reviews, and (2) the individual or combined use of the following keywords: “genetic variation”, “nutrigenetics”, “precision nutrition”, “couple’s subfertility”, and “couple’s infertility”. A preliminary reading of all the articles was carried out, and only those that best fit the themes and subthemes of the narrative review were selected. Results: Scientific evidence suggests that adherence to a healthy diet that follows the Mediterranean pattern is associated with increased fertility in women and improved semen quality in men, better metabolic health and reduced levels of inflammation and oxidative stress, as well as maintaining a healthy body weight. The integration of different tools, such as nutrigenetics, predictive biochemical analyses, intestinal microbiota tests and clinical nutrition software, used in precision nutrition interventions can contribute to providing information on how diet and genetics interact and how they can influence fertility. Conclusions: The adoption of a multidisciplinary and precision approach allows the design of dietary and lifestyle recommendations adapted to the specific characteristics and needs of couples with subfertility, thus optimizing reproductive health outcomes and achieving successful conception.