bluuebunny/crossref_metadata_2025 huggingface dataset:
Research progress and latest application of ultrasonic treatment on protein extraction and modification
Authors: Zhang Jianyou , Ji Shengqiang , Liang Jianqin , Chen Yutong , Tang Wei , Lyu Fei
Published: June 2024

The advancement of the modern food industry has prompted a shift away from traditional protein extraction methods due to their inherent limitations. As a result, there is a growing interest in exploring alternative methods for protein extraction and modification such as ultrasonic (US) treatment. This shift towards more sustainable and efficient protein extraction methods is a key research focus in obtaining safe proteins in an environmentally friendly manner. Furthermore, enhancing the functional properties of proteins through modification can expand their application. This paper reviews recent research progress and applications of US treatment on protein extraction and modification as well as the impact on protein structure and functional properties. In addition, the latest applications of ultrasound‐modified‐proteins, including nano‐proteins, 3D printing and interactions between proteins and other food components, are reviewed. This review may provide some inspiration for the potential application of US technologies in food fields.
The role of pan‐roasting on changes in bioactive compounds, phenolic and fatty acid composition of chia (Salvia hispanica L.) seeds
Authors: Mohamed Ahmed Isam A. , AlJuhaimi Fahad , Özcan Mehmet Musa , Uslu Nurhan , Karrar Emad
Published: June 2024

In this study, the effect of roasting on the oil quantities, bioactive properties, colour, fatty acids and polyphenols of seeds roasted in pan at roasting times was investigated. Total phenolic and flavonoid quantities of roasted seeds are assayed among 186.59 (control) and 429.29 mgGAE/100 g (20 min) to 810.00 (10 min) and 903.33 mg/100 g (5 min), respectively. The heat treatment increased the oil and total phenol quantities of the chia seeds compared to the control, depending on the increase in roasting time. In addition, the antioxidant effect of the seeds in the 5‐min roasting process was the same as in the control, but it showed differences with other roasting times. As the roasting time increased, a significant increase was observed in the L* values of the seeds and this reduced the brightness of the seeds. The 3,4‐dihydroxybenzoic acid and catechin quantities of the chia seeds roasted for 15 and 20 min were higher than the 3,4‐dihydroxybenzoic acid and catechin amounts of control and chia seeds roasted for 5 and 10 min. Linolenic acid was found to be the highest in chia oils, and its amount changed between 63.66 (20 min) and 66.88% (control).
Insights into the roles of heat‐stable protein derived from egg white in vitamin A stabilisation
Authors: Gao Yang , Liu Hanhan , Wang Ruoyong , Zang Jiachen , Lv Chenyan
Published: June 2024

Vitamin A (VA) is a fat‐soluble compound that is essential for physiological functions including vision, fetal growth, immune response, cell differentiation and proliferation. However, its susceptibility to environmental conditions leading to poor stability limits its application in food. This study investigated the interaction between egg white protein (EWP) and VA by spectroscopy and molecular docking. The results indicated one EWP could bind 16.00 ± 0.89 VA molecules and the binding constant is (2.59 ± 0.71) × 105 m−1. What's more, there was a fluorescence resonance energy transfer between EWP and VA, and the Förster radius is 5.28 nm. In addition, VA enhanced the hydrophobic interactions with the EWP, resulting in protein aggregation, larger average particle size and more uniform particle size distribution. Finally, the combination of VA with EWP could significantly improve the photothermal stability and storage stability of VA. These studies indicate that EWP nanocomplexes can be used as ideal delivery carriers for bioactive substances.
The effects of white noise and swaddling methods on orogastric tube insertion‐related pain in preterm infants: A randomized controlled trial
Authors: Kirli Ceyda , Kisacik Öznur Gürlek , Gürel Selçuk
Published: June 2024

Aim

This study aims to investigate the effects of the white noise, swaddling and white noise + swaddling methods on pain and physiological parameters associated with orogastric tube insertion procedure.

Methods

This was a randomized controlled trial. A total of 132 preterm infants were randomly assigned to four groups as white noise group (n = 33), swaddling group (n = 33), white noise + swaddling group (n = 33) and control group (n = 33). Interventions were initiated 5 min before the orogastric tube insertion procedure and continued during and up to 5 min after the procedure.

Results

White noise intervention alone did not have a significant effect on reducing pain associated with orogastric tube insertion (p > 0.05). Compared with the control group, the preterm infants in the swaddling group experienced 0.587 times less pain, and those in the white noise + swaddling group experienced 0.473 times less pain.

Conclusions

Findings indicate the swaddling and the combination of white noise + swaddling may be a useful intervention in reducing the invasive pain experienced by preterm infants during and after orogastric tube insertion and in improving the physiological parameters associated with pain.
Taxonomic and phylogenetic β‐diversity of regional assemblages of angiosperm species in relation to geographic and climatic determinants in Africa
Authors: Qian Hong , Jin Yi , Deng Tao
Published: June 2024

Aim

Beta‐diversity quantifies the change in taxonomic and phylogenetic composition between areas, and is a scalar between local (α) and regional (γ) diversity. Geographic distance, which reflects dispersal limitation, and climatic distance, which reflects environmental filtering, are major drivers of β‐diversity. Here, we analyse a comprehensive data set of angiosperms in regional floras across Africa to assess the relationships of β‐diversity, and its components, to three major types of environmental variables (current climate, Quaternary climate change and topographic heterogeneity) thought to drive β‐diversity.

Location

Africa.

Taxon

Angiosperms.

Methods

Africa was divided into 27 regions. Species lists of angiosperms for each region were collated. The relationships of both taxonomic and phylogenetic β‐diversity, and their respective turnover and nestedness components, with geographic and environmental distances were assessed.

Results

This study showed that (1) regions of the lowest β‐diversity are located in moist tropical climates, (2) the turnover and nestedness components of β‐diversity are negatively correlated with each other, (3) taxonomic β‐diversity is higher than phylogenetic β‐diversity across Africa, (4) variation in β‐diversity of angiosperms is more strongly associated with current climate than with Quaternary climate change and topographic heterogeneity and (5) the variation in taxonomic β‐diversity and its turnover component that is independently explained by geographic distance is much larger than that is independently explained by climatic distance for angiosperms in Africa.

Main Conclusions

The finding that geographic distance explained more variation than climatic distance suggests that dispersal limitation plays a greater role than environmental filtering in shaping angiosperm β‐diversity in Africa. Of climatic factors, current climate plays a more important role than Quaternary climate change in shaping angiosperm β‐diversity in Africa.
The use of phages for the biosynthesis of metal nanoparticles and their biological applications: A review
Authors: Hosseininasab Seyed Soheil , Naderifar Mahin , Akbarizadeh Majid Reza , Rahimzadeh Mohammadjavad , Soltaninejad Simin , Makarem Zohre , Satarzadeh Naghmeh , Sadeghi Dousari Amin
Published: June 2024

Nowadays, the use of biological methods of synthesis of nanoparticles as substitutes for methods that use high energy and consumption of expensive and dangerous materials is of interest to researchers all over the world. Biological methods of synthesising metal nanoparticles are very important because they are easy, affordable, safe, environmentally friendly and able to control the size and shape of nanoparticles. One of the methods that is of interest today is the use of bacteriophages as the most abundant organisms in nature in the synthesis of metal nanoparticles. Nanomaterials biosynthesized from phages have shown various clinical applications, including antimicrobial activities, biomedical sensors, drug and gene delivery systems, cancer treatment and tissue regeneration. Therefore, the purpose of this review is to investigate the biosynthesis of metal nanoparticles with phages and their biomedical applications.
The expansion of invasive species to the East: new sites of the bullheads (genus Ameiurus Rafinesque 1820) in Ukraine with morphological and genetic identification
Authors: Kutsokon Yuliia , Bielikova Olena , Pekárik Ladislav , Roman Anatolii , Shcherbatiuk Mykola , Čiamporová‐Zaťovičová Zuzana , Čiampor Fedor
Published: June 2024

This study confirms the extended distribution of two invasive species of the genus Ameiurus in Ukraine. Specifically, A. melas is recorded for the first time in the Southern Buh basin and A. nebulosus has expanded further eastward within the Dnipro basin. Material collected in 2019 and 2022 was identified by morphological features and confirmed by molecular genetic analysis. The most reliable morphological characters for distinguishing these two species include anal‐fin membrane pigmentation (light or black), gill raker count (fewer or more than 16), and serrations on the pectoral‐fin spine (well‐developed along the full length or small, absent near the tip). The analysis of the cytochrome oxidase subunit I barcoding marker identified all samples from the Dnipro Basin (Tnia and Velykyi Luh localities) as A. nebulosus, while all specimens from the Vinnytsia region within the Southern Buh basin (Sotskoho and Vyshenske lakes) were attributed to A. melas. The maximum‐likelihood analysis revealed clearly separated clades with high bootstrap support (>75%), strongly supporting the presence of the two separate species. This study suggests the potential for further eastward expansion of both species within Ukraine: A. nebulosus in the northern direction and A. melas in the southern direction.
Cypermethrin exposure: Implications for growth, phenotypic attributes, and biochemical responses in monosex tilapia (Oreochromis niloticus)
Authors: Hazra Prema , Ghosh Alokesh Kumar , Sarower Md. Golam
Published: June 2024

The present study was conducted to evaluate the effects of exposure to sublethal concentrations of cypermethrin on growth, phenotypic traits, and metabolic and antioxidant enzymes activities of monosex tilapia (Oreochromis niloticus). The median lethal concentration (LC50) of cypermethrin was initially ascertained to be 0.04 ppm after 12 h of exposure to three concentrations (0.04, 0.20, and 1.0 ppm) via the Probit analysis method. The sublethal exposures were obtained for 3 months at 0.0016, 0.008, and 0.04 ppm of cypermethrin. The growth performance, phenotypic traits, and enzymatic activity were analyzed. The research findings revealed a notable impact of cypermethrin on the growth performance of monosex tilapia, with a significant (p < 0.05) decrease in weight observed as the concentration increased. The experiment documented a significant change (p < 0.05) in the phenotypic traits of standard length, total length, tail length, trunk length, eye diameter, body area, and color patterns, but not dressing percentage. Furthermore, it was observed that the activities of amylase and protease in both the intestine and muscle decreased significantly (p < 0.05) with increasing concentrations of cypermethrin, except for amylase in the muscle, where no significant change was noted (p > 0.05). The results also demonstrated significant reductions (p < 0.05) in the activity of metabolic enzymes, specifically superoxide dismutase and catalase, following exposure to sublethal levels of cypermethrin. The study suggested that sublethal exposure of pyrethroid alters the growth, phenotypic traits, metabolic enzymes activities, and immunity of monosex tilapia.
Updates in Medical and Surgical Weight Loss
Authors: Davis Melissa G. , Sanders Bethany D.
Published: June 2024

The number of individuals with obesity is at an all‐time high, and the rate of obesity continues to climb each year. Obesity is a chronic disease with widespread effects throughout the body. Midwives and perinatal care providers need an understanding of the etiology, pathophysiology, and interventions for obesity. A review of evidence‐based diet and lifestyle modifications, medications, and surgical procedures is presented.
Towards autistic flow theory: A non‐pathologising conceptual approach
Authors: Heasman Brett , Williams Gemma , Charura Divine , Hamilton Lorna G. , Milton Damian , Murray Fergus
Published: June 2024

Flow states are heightened moments of concentration, motivation and enjoyment, leading to total absorption in the present moment. A striking parallel exists between flow states and phenomenological accounts of autistic daily life. We analyse the components of flow theory alongside autistic autobiographical accounts to explore similarities and differences, in doing so moving toward an understanding of autistic flow theory. We highlight the considerations and opportunities this may hold for future autism research, in particular the advantage that this offers a non‐pathologising approach to researching autism, one which helps to explain contextualised behaviour (i.e., alignment between the situation and what is happening in one's mind). Drawing on autistic autobiographical accounts, we outline four principles: (1) autistic people are uniquely placed to discover and manage flow; (2) autistic flow may qualitatively diverge from traditional models of flow; (3) difficulties maintaining and exiting flow for autistic people highlight a need to examine transitions into and out of flow; and, (4) internal and external constraints to flow highlight there is unrealised autistic potential yet to be discovered. The implications of an autistic flow theory are discussed in terms of how it can impact (a) our conceptual understanding of autism providing alternative explanations to previously researched phenomena, and (b) how we build enabling environments for autistic people that allow flow to flourish across educational practice, wellbeing and research contexts.
Lizards and the enzootic cycle of Borrelia burgdorferi sensu lato
Authors: Nowak Tristan A. , Burke Russell L. , Diuk‐Wasser Maria A. , Lin Yi‐Pin
Published: June 2024

Emerging and re‐emerging pathogens often stem from zoonotic origins, cycling between humans and animals, and are frequently vectored and maintained by hematophagous arthropod vectors. The efficiency by which these disease agents are successfully transmitted between vertebrate hosts is influenced by many factors, including the host on which a vector feeds. The Lyme disease bacterium Borrelia burgdorferi sensu lato has adapted to survive in complex host environments, vectored by Ixodes ticks, and maintained in multiple vertebrate hosts. The versatility of Lyme borreliae in disparate host milieus is a compelling platform to investigate mechanisms dictating pathogen transmission through complex networks of vertebrates and ticks. Squamata, one of the most diverse clade of extant reptiles, is comprised primarily of lizards, many of which are readily fed upon by Ixodes ticks. Yet, lizards are one of the least studied taxa at risk of contributing to the transmission and life cycle maintenance of Lyme borreliae. In this review, we summarize the current evidence, spanning from field surveillance to laboratory infection studies, supporting their contributions to Lyme borreliae circulation. We also summarize the current understanding of divergent lizard immune responses that may explain the underlying molecular mechanisms to confer Lyme spirochete survival in vertebrate hosts. This review offers a critical perspective on potential enzootic cycles existing between lizard‐tick‐Borrelia interactions and highlights the importance of an eco‐immunology lens for zoonotic pathogen transmission studies.
Host lifestyle and parasite interspecific facilitation mediate co‐infection in a species‐poor host–parasite system
Authors: Kmentová Nikol , Cruz‐Laufer Armando J. , Milec Leona J. M. , Moons Tanisha , Heeren Senne , van den Hoorn Elze , Thys Kelly J. M. , Makasa Lawrence , Chocha Manda Auguste , Masilya Mulungula Pascal , Van Steenberge Maarten , Jorissen Michiel W. P. , Vanhove Maarten P. M.
Published: June 2024

Despite their important ecological role, questions remain on mechanisms structuring parasite assemblages. We present an endemic and species‐poor parasite–host system of two species of clupeid fishes Limnothrissa miodon, Stolothrissa tanganicae) and two species of monogenean parasites (Kapentagyrus, Dactylogyridae) from Lake Tanganyika as a model to study parasite distribution patterns and co‐infection dynamics in nature. We modelled spatiotemporal dynamics of parasite–host interaction using infection data along the north–south axis of Lake Tanganyika (660 km) over the course of two seasons and four years (1730 fish, 3710 parasites). We found temporal stability of parasite infection, which contrasts with previously reported seasonally driven fluctuations of fish host abundances. We found a difference in spatial structure between the parasite species which follows the contrasting dispersal pattern of their respective host species. On L. miodon, the host species that is infected by the two parasite species, we discovered a positive correlation with host body size for one parasite species, and a negative correlation for the other species. The apparent host‐size related replacement in the two parasite species infecting L. miodon contrasts with the beneficial influence that each of the parasite species has on the other. Parasites have been reported in previous studies to change habitat depending on host size/age. Differences in abundance and prevalence here reported between parasite species infecting the same host species related to the host body length further illustrate the parasites' dependence on changes in lifestyle of the host during its ontogenetic development. Also, possible mechanisms underlying the reported facilitation of infection are discussed. In conclusion, we show that in this closed, species‐poor system parasite infection dynamics are dependent on a combination of host mobility, host lifestyle changes over ontogenetic development, and interspecific interactions between parasites.
Thermal habitat fragmentation in stratified lakes induces resource waves that brook charr track across seasons
Authors: Pépino Marc , Magnan Pierre , Leroux Riwan , Bertolo Andrea
Published: June 2024

The spatial configuration of thermal habitats constrains the thermoregulatory performance of ectotherms. Thermal landscapes also vary through time, which is particularly relevant in seasonal environments such as temperate lakes. Indeed, elevated temperatures in the epilimnion of dimictic lakes during summer could substantially reduce the use of this habitat by cold‐stenothermic fish during the stratified period. The main objective of this study was to evaluate whether thermal habitat fragmentation in stratified lakes modulates accessibility to resources that brook charr, Salvelinus fontinalis, which is a mobile consumer, can track across seasons. More specifically, we hypothesize that reduced access to the littoral habitat during summer enhances foraging opportunities in this habitat during winter. We used an automatic acoustic telemetry system offering full coverage of the lake to continuously record brook charr locations across seasons, and we estimated zoobenthos abundances in the littoral habitat using image processing and semi‐automatic classification. While brook charr concentrate in the metalimnion of the pelagic habitat in summer, most individuals in winter shift to a shallow bay that is unexploited in summer due to thermal constraints. In this habitat, zoobenthos abundance is more than twice as high at the end of the summer compared to littoral habitats close to the thermal refuge in the pelagic habitat. Surprisingly, brook charr showed strong within‐lake site fidelity between two consecutive summers, which suggests that spatial memory could be a key driver of seasonal habitat use in this lacustrine population. Overall, our results suggest that thermal barriers create fragmentation between littoral and pelagic habitats that in turn produces resource opportunities that brook charr can track across seasons.
Detailed observations of the life history and phylogenetic placements of the freshwater green alga Oocystaenium elegans (Oocystaceae, Trebouxiophyceae), with an emended description
Authors: Mizobuchi Aya , Handa Shinji , Tsubota Hiromi
Published: June 2024

Oocystaenium elegans Gonzalves & Mehra (Oocystaceae, Trebouxiophyceae) was collected from a paddy field in Japan. Its life history was reexamined based on detailed observations of cultured material, and an emended description is given. This species is a large spindle‐shaped green alga that grows in puddles and paddy fields and has been reported in India, Nepal and Cuba. Reproduction occurs asexually via autospore formation and sexually via oogamy. Although this oogamy is considered to involve fertilization by globular egg cells and antherozoids, the results of the present study revealed that the egg cells (female cells) remained spindle‐shaped and became globular zygotes after fertilization. The place on the egg cells where the antherozoid attached swelled to become a receptive papilla, after which the antherozoid invaded. Phylogenetic analysis based on nuclear 18S small subunit ribosomal RNA gene sequences showed that O. elegans is included in the family Oocystaceae.
Lack of causative mutation in the AMH and AMHR2 genes in a cat (38,XY) with persistent Mullerian duct syndrome (PMDS)
Authors: Rozynek Jedrzej , Nowacka‐Woszuk Joanna , Stachowiak Monika , Sowinska Natalia , Lukomska Anna , Gruss Michal , Switonski Marek , Szczerbal Izabela
Published: June 2024

A 1‐year‐old European shorthair male cat with a normally developed penis was subjected to genetic, endocrinological and histological studies due to unilateral cryptorchidism. The blood testosterone level was typical for males, while the level of anti‐Mullerian hormone (AMH) was very low. Surgical removal of internal reproductive organs was followed by a histological study, which revealed inactive testicles with neoplastic changes and derivatives of Mullerian ducts. Cytogenetic analysis showed a normal XY sex chromosome complement and molecular analysis confirmed the presence of Y‐linked genes (SRY and ZFY). Although the level of AMH was low, two normal copies of the AMH gene were found using droplet digital PCR (ddPCR). Analysis of the coding sequences of two candidate genes (AMH and AMHR2) for persistent Mullerian duct syndrome (PMDS) in the affected cat and in control male cats (n = 24) was performed using the Sanger sequencing method. In the affected cat, homozygosity was found for three novel missense variants in Exon 1 (one SNP) and Exon 5 (two SNPs) of AMH, but the same homozygous genotypes were also observed in one and two control cats, respectively, whose sex development was not examined. Three known synonymous variants with homozygous status were found in AMHR2. We conclude that the DNA variants identified in AMH and AMHR2 are not responsible for PMDS in the affected cat.
Interconnected and resilient: A CGE analysis of AI‐driven cyberattacks in global trade
Authors: Osman Rehab , El‐Gendy Sherif
Published: June 2024

The burgeoning interconnectedness of global trade in the digital age not only presents enticing opportunities but also harbors potent vulnerabilities of artificial intelligence (AI)‐driven cyberattacks. This study explores the cascading impacts of these disruptive threats on economies, supply chains, and trade, utilizing the intricate lens of Computable General Equilibrium modeling.

Through meticulously designed simulation scenarios, we illuminate the potential economic ramifications of cyberattacks, with a focus on regions heavily reliant on digital technologies and interwoven supply chains. The analysis reveals significant declines in real GDP, trade prices and volumes, and trade route disruptions across regions. Notably, economies like China, the United States, the United Kingdom, and the EU, due to their deep integration in global networks, face pronounced vulnerabilities.

However, amidst this bleak landscape, hope emerges in the form of cyber resilience. The study showcases the effectiveness of proactive measures like adaptable production systems, diversified trade partners, and robust cybersecurity infrastructure in mitigating the adverse impacts of cyberattacks. Incorporating cyber resilience significantly dampens the reported negative consequences, highlighting the critical role of preparedness in combating digital warfare.

This study underscores the urgent need for a global paradigm shift toward cyber resilience. Collective efforts to bolster cybersecurity infrastructures, foster international cooperation in threat intelligence, and establish open and resilient trade frameworks are crucial in navigating the treacherous labyrinth of AI‐driven cyberattacks. By embracing resilience strategies and fostering global collaboration, we can pave the way for a more secure and prosperous digital future, where interconnectedness becomes a tool for progress, not a vulnerability to be exploited.
Effect of selective trimming of nasoavleolar molding on maxillary arch growth in patients with unilateral cleft lip and palate
Authors: Murthy Prashanth Sadashiva , Venkatapathy Aarthi Latha , Deshmukh Seema
Published: June 2024

Aim

Presurgical Nasoalveolar molding (PNAM) is a technique used for cleft lip and palate patients prior to cheiloplasty. However, concerns exist regarding its negative impact on maxillary arch growth.This study aimed to assess the effect of selective trimming in NAM on maxillary arch growth in patients with unilateral cleft lip palate.

Methodology

The retrospective observational study analyzed the study casts of 30 patients before and after undergoing nasoalveolar molding treatment. Study casts which were repositories of the institute were analyzed and segregated as Group A: NAM given with selective trimming of the appliance, and Group B: NAM without selective trimming of the appliance. Pre and post‐treatment casts were assessed digitally at L‐L’ (Intercleft segment width), C‐C’ (Intercanine width), T‐T’ (Alveolar arch width), L‐TT’ (Alveolar arch length from major cleft segment), and L’‐TT’ (Alveolar arch length from minor cleft segment)

Results

A notable significant difference between Group A's and B's mean Alveolar arch width and Intercleft segment width was seen. Whereas parameters such as intercanine width, and alveolar arch length as functions from major and minor segments showed no significant variance.

Conclusion

Although NAM has been known to affect the growth of the maxillary arch, this study proposes that techniques such as selective trimming can help counteract this drawback.
A data‐driven genome annotation approach for cassava
Authors: Chenna Swetha , Ivanov Maxim , Nielsen Tue Kjærgaard , Chalenko Karina , Olsen Evy , Jørgensen Kirsten , Sandelin Albin , Marquardt Sebastian
Published: June 2024

Genome annotation files play a critical role in dictating the quality of downstream analyses by providing essential predictions for gene positions and structures. These files are pivotal in decoding the complex information encoded within DNA sequences. Here, we generated experimental data resolving RNA 5′‐ and 3′‐ends as well as full‐length RNAs for cassava TME12 sticklings in ambient temperature and cold. We used these data to generate genome annotation files using the TranscriptomeReconstructoR (TR) tool. A careful comparison to high‐quality genome annotations suggests that our new TR genome annotations identified additional genes, resolved the transcript boundaries more accurately and identified additional RNA isoforms. We enhanced existing cassava genome annotation files with the information from TR that maintained the different transcript models as RNA isoforms. The resultant merged annotation was subsequently utilized for comprehensive analysis. To examine the effects of genome annotation files on gene expression studies, we compared the detection of differentially expressed genes during cold using the same RNA‐seq data but alternative genome annotation files. We found that our merged genome annotation that included cold‐specific TR gene models identified about twice as many cold‐induced genes. These data indicate that environmentally induced genes may be missing in off‐the‐shelf genome annotation files. In conclusion, TR offers the opportunity to enhance crop genome annotations with implications for the discovery of differentially expressed candidate genes during plant–environment interactions.
A plasma membrane‐localized transporter remobilizes aleurone layer magnesium for seed germination in rice
Authors: Guan Liurong , Yin Li , Liu Yingna , Yan Jun , Wang Bin , Luan Mingda , Lan Wenzhi
Published: June 2024

The aleurone layer in cereal grains acts as a major reservoir of essential mineral nutrients, significantly influencing seed germination. However, the molecular mechanism underlying the redistribution of nutrients from the aleurone layer in the germinating seed is still not well understood. Here, in rice, we identified a plasma membrane (PM) localized magnesium transporter, MAGNESIUM RELEASE TRANSPORTER 3 (MGR3), is critical for seed germination. OsMGR3 is predominantly expressed in the aleurone layer cells of endosperm, facilitating magnesium remobilization during germination. Non‐invasive Micro‐test Technology assay data demonstrated that the loss‐of‐function of OsMGR3 restrained magnesium efflux from the aleurone layer. In the embryo/endosperm grafting experiment, we observed that the mutation of OsMGR3 in the aleurone layer suppressed the growth and differentiation of the embryo during germination. Furthermore, magnesium fluorescence imaging revealed the osmgr3 mutant seeds showed impaired exportation of aleurone layer‐stored magnesium to the embryo, consequently delaying germination. Importantly, we discovered that disrupting OsMGR3 could inhibit pre‐harvest sprouting without affecting rice yield and quality. Therefore, the magnesium efflux transporter OsMGR3 in the aleurone layer represents a promising genetic target for future agronomic trait improvement.
BREATHE DMD: boosting respiratory efficacy after therapeutic hypoxic episodes in Duchenne muscular dystrophy
Authors: Ó Murchú Seán C. , O'Halloran Ken D.
Published: June 2024

Duchenne muscular dystrophy (DMD) is a fatal genetic neuromuscular disorder, characterised by progressive decline in skeletal muscle function due to the secondary consequences of dystrophin deficiency. Weakness extends to the respiratory musculature, and cardiorespiratory failure is the leading cause of death in men with DMD. Intermittent hypoxia has emerged as a potential therapy to counteract ventilatory insufficiency by eliciting long‐term facilitation of breathing. Mechanisms of sensory and motor facilitation of breathing have been well delineated in animal models. Various paradigms of intermittent hypoxia have been designed and implemented in human trials culminating in clinical trials in people with spinal cord injury and amyotrophic lateral sclerosis. Application of therapeutic intermittent hypoxia to DMD is considered together with discussion of the potential barriers to progression owing to the complexity of this devastating disease. Notwithstanding the considerable challenges and potential pitfalls of intermittent hypoxia‐based therapies for DMD, we suggest it is incumbent on the research community to explore the potential benefits in pre‐clinical models. Intermittent hypoxia paradigms should be implemented to explore the proclivity to express respiratory plasticity with the longer‐term aim of preserving and potentiating ventilation in pre‐clinical models and people with DMD.

image
The Quest for Better Batteries
Authors: Apte Poornima
Published: June 2024

Challenges involving safety and supply chain bottlencks have spurred private companies and university research teams to find alternatives to the lithium-ion battery that powers electronics and automobiles. New chemistries and internal structures may enable engineers to develop products to take advantage of advanced capabilities offered by solid-state and sodium batteries.
CRFP Mechanical Properties—Stated Values Versus Experimental Data
Authors: Jribi Kais , Azizi Boutros , Mello Alberto W.
Published: June 2024

This study thoroughly examines the mechanical properties of carbon fiber-reinforced polymers (CFRPs), motivated by the critical need for accurate composite property data in investigating fracture control measures for structures subjected to barely visible impact damage. We compared experimental results with manufacturer-stated values, focusing on discrepancies in fiber volume fraction and its impact on elastic modulus. Experimental findings showed an increase in elastic modulus to 190 GPa for 0 deg orientation samples, compared to the manufacturer's stated value of 159.27 GPa. The recalculated fiber volume fraction increased from the expected 57% to an actual value of 60.96%. This increase in fiber content, determined through the Voigt modulus equation and corroborated by SEM image analysis, directly contributed to the observed variations in elastic modulus. Tension tests at 0 deg and 90 deg angles exhibited average percentage errors of 14.83% and 11.57%, respectively, while compression tests at 0 deg displayed a deviation of approximately −13.92% after adjusting for values beyond 0.05% compressive strain. The study underscores the critical impact of fiber volume fraction on CFRP properties and highlights the importance of precise empirical evaluation for accurate CFRP characterization in applications such as aerospace engineering.
Microstructure, Mechanical Properties, and Wear Behavior of Dissimilar Metallic Coatings for Steel Discs of Butterfly Valves
Authors: Lähdeniemi Juuso , Chen Jiayi , Teittinen Tuomas , Yeddu Hemantha Kumar
Published: June 2024

In the present work, coating materials that can be welded to the EN 1.6220 low-alloy steel disc of a butterfly valve and are also compatible with the seal material, i.e., 17–4 PH steel, are studied. 312 duplex stainless steel, 316 austenitic stainless steel, and Stellite 6 are identified as potential coating materials for the disc based on Cobweb analysis and are welded to the disc using metal active gas (MAG) welding (312 and 316 steel coatings) and powder plasma arc welding (Stellite 6 coating). Microstructural analyses and Vickers hardness measurements of the weld joints are performed. The surface roughness and wear behavior of the coatings are also studied. Nanoscale wear phenomena and consequent phase transformations are studied using molecular dynamics simulations. The results show that 312 and 316 stainless steels are suitable coating materials for the disc.
A Review of Computational Fluid Dynamics Approaches Used to Investigate Lubrication of Rolling Element Bearings
Authors: Sadeghi Farshid , Arya Ujjawal , Aamer Saeed , Meinel Andreas
Published: June 2024

Optimizing bearing performance is based on effective lubrication, especially in high-speed machinery, where minimizing churning and drag losses is of significant importance. Over the past few decades, extensive research has been conducted into the better understanding of different aspects of bearing lubrication. These investigations have employed a combination of experimental methods and advanced computational fluid dynamics (CFD) models. This article provides a comprehensive overview of critical aspects of bearing lubrication, with a specific emphasis on recent advances in CFD models. Lubricant flow and distribution patterns are discussed while examining their impact on drag and churning losses. An extensive discussion is provided on the meshing strategies and modeling approaches used to simulate various flow phenomena within bearings. In addition, relevant trends and impacts of cage design on bearing lubrication and fluid friction have been explored, along with a discussion of prevailing limitations that can be addressed in future bearing CFD models.
Dynamic Modeling and Experimental Modal Analysis for the Central Rod-Fastened Rotor With Hirth Couplings Based on Fractal Contact Theory
Authors: Huang Gancai , Liu Chao , Jiang Dongxiang
Published: June 2024

The central rod-fastened rotor of gas turbine exhibits pronounced noncontinuous characteristics due to the large number of contact interfaces between the compressor and turbine disks. It is necessary to establish an accurate dynamic modeling method for the central rod-fastened rotor that fully considers the contact surface effect. In this work, the contact behavior of the rough surface is characterized by the fractal theory. The normal and tangential contact stiffness models are developed, and the influence of fractal parameters is discussed. Besides, the finite element model for the central rod-fastened rotor is established by developing an improved contact element considering the equivalent stiffness segment of Hirth couplings. Finally, the proposed model is verified by conducting the modal testing and measuring the first four modes of natural frequencies and modal shapes of the central rod-fastened rotor. The results show that the numerical results are in good agreement with the experimental ones, and the fractal contact model can effectively predict the connection stiffness of Hirth couplings, which in turn improves the simulation accuracy for the modal characteristics of the central rod-fastened rotor and provides a dynamic modeling approach with high efficiency and less computational complexity.
Reliability of Three Landmine-Punch-Throw Variations and Their Load–Velocity Relationships Performed With the Dominant and Nondominant Hands
Authors: Omcirk Dan , Vetrovsky Tomas , O’Dea Cian , Ruddock Alan , Wilson Daniel , Maleček Jan , Padecky Jan , Janikov Martin Tino , Tufano James J.
Published: June 2024

Purpose: This study assessed the reliability and load–velocity profiles of 3 different landmine-punch-throw variations (seated without trunk rotation, seated with trunk rotation, and standing whole body) with different loads (20, 22.5, and 25.0 kg), all with the dominant hand and nondominant hand. Methods: In a quasi-randomized order, 14 boxers (24.1 [4.3] y, 72.6 [10.1] kg) performed 3 repetitions of each variation with their dominant hand and their nondominant hand, with maximal effort and 3 minutes of interset rest. Peak velocity was measured via the GymAware Power Tool (Kinetic Performance Technologies). The interclass correlation coefficients and their 95% CIs were used to determine the intrasession reliability of each variation × load × hand combination. Additionally, a 2 (hand) × 3 (variation) repeated-measures analysis of variance assessed the load–velocity profile slope, and a 3 (variation) × 2 (hand) × 3 (load) repeated-measures analysis of variance assessed the peak velocity of each variation. Results: Most variations were highly reliable (intraclass correlation coefficient > .91), with the nondominant hand being as reliable or more reliable than the dominant hand. Very strong linear relationships were observed for the group average for each variation (R2 ≥ .96). However, there was no variation × hand interaction for the slope, and there was no main effect for variation or hand. Additionally, there was no interaction for the peak velocity, but there were main effects for variation, hand, and load (P < .01). Conclusion: Each variation was reliable and can be used to create upper-body ballistic unilateral load–velocity profiles. However, as with other research on load–velocity profile, individual data allowed for more accurate profiling than group average data.
Evaluation of commercial, customized microdilution plates for
Ureaplasma parvum
,
Ureaplasma urealyticum
, and
Mycoplasma hominis
antimicrobial susceptibility testing and determination of antimicrobial resistance prevalence in France
Authors: Pereyre Sabine , Hénin Nadège , Dolzy Amandine , Guiraud Jennifer , Laurier-Nadalié Cécile , Gardette Marie , Bébéar Cécile ,   for the investigator group
Published: June 2024

Antimicrobial susceptibility testing (AST) of human mycoplasmas using microdilution is time-consuming. In this study, we compared the performance of MICRONAUT-S plates (Biocentric-Bruker) designed for AST of
Ureaplasma parvum
,
Ureaplasma urealyticum
, and
Mycoplasma hominis
with the results using the Clinical & Laboratory Standards Institute (CLSI) reference method. Then, we investigated the prevalence and mechanisms of resistance to tetracyclines, fluoroquinolones, and macrolides in France in 2020 and 2021. The two methods were compared using 60 strains. For the resistance prevalence study,
U. parvum
-,
U. urealyticum
-, and
M. hominis
-positive clinical specimens were collected for 1 month each year in 22 French diagnostic laboratories. MICs were determined using the MICRONAUT-S plates. The
tet
(M) gene was screened using PCR, and fluoroquinolone resistance-associated mutations were screened using PCR and Sanger sequencing. Comparing the methods, 99.5% (679/680) MICs obtained using the MICRONAUT-S plates concurred with those obtained using the CLSI reference method. For 90
M
.
hominis
isolates, the tetracycline, levofloxacin, and moxifloxacin resistance rates were 11.1%, 2.2%, and 2.2%, respectively, with no clindamycin resistance. For 248
U
.
parvum
isolates, the levofloxacin and moxifloxacin resistance rates were 5.2% and 0.8%, respectively; they were 2.9% and 1.5% in 68
U
.
urealyticum
isolates. Tetracycline resistance in
U. urealyticum
(11.8%) was significantly (
P
< 0.001) higher than in
U. parvum
(1.2%). No macrolide resistance was observed. Overall, the customized MICRONAUT-S plates are a reliable, convenient tool for AST of human mycoplasmas. Tetracycline and fluoroquinolone resistance remain limited in France. However, the prevalence of levofloxacin and moxifloxacin resistance has increased significantly in
Ureaplasma
spp. from 2010 to 2015 and requires monitoring.

IMPORTANCE

Antimicrobial susceptibility testing of human urogenital mycoplasmas using the CLSI reference broth microdilution method is time-consuming and requires the laborious preparation of antimicrobial stock solutions. Here, we validated the use of reliable, convenient plates designed for antimicrobial susceptibility testing that allows the simultaneous determination of the MICs of eight antibiotics of interest. We then investigated the prevalence and mechanisms of resistance of each of these bacteria to tetracyclines, fluoroquinolones, and macrolides in France in 2020 and 2021. We showed that the prevalence of levofloxacin and moxifloxacin resistance has increased significantly in
Ureaplasma
spp. from 2010 to 2015 and requires ongoing monitoring.
Warming alters cascading effects of a dominant arthropod predator on fungal community composition in the Arctic
Authors: Koltz Amanda M. , Koyama Akihiro , Wallenstein Matthew
Published: June 2024

Rapid climate change in the Arctic is altering microbial structure and function, with important consequences for the global ecosystem. Emerging evidence suggests organisms in higher trophic levels may also influence microbial communities, but whether warming alters these effects is unclear. Wolf spiders are dominant Arctic predators whose densities are expected to increase with warming. These predators have temperature-dependent effects on decomposition via their consumption of fungal-feeding detritivores, suggesting they may indirectly affect the microbial structure as well. To address this, we used a fully factorial mesocosm experiment to test the effects of wolf spider density and warming on litter microbial structure in Arctic tundra. We deployed replicate litter bags at the surface and belowground in the organic soil profile and analyzed the litter for bacterial and fungal community structure, mass loss, and nutrient characteristics after 2 and 14 months. We found there were significant interactive effects of wolf spider density and warming on fungal but not bacterial communities. Specifically, higher wolf spider densities caused greater fungal diversity under ambient temperature but lower fungal diversity under warming at the soil surface. We also observed interactive treatment effects on fungal composition belowground. Wolf spider density influenced surface bacterial composition, but the effects did not change with warming. These findings suggest a widespread predator can have indirect, cascading effects on litter microbes and that effects on fungi specifically shift under future expected levels of warming. Overall, our study highlights that trophic interactions may play important, albeit overlooked, roles in driving microbial responses to warming in Arctic terrestrial ecosystems.

IMPORTANCE

The Arctic contains nearly half of the global pool of soil organic carbon and is one of the fastest warming regions on the planet. Accelerated decomposition of soil organic carbon due to warming could cause positive feedbacks to climate change through increased greenhouse gas emissions; thus, changes in ecological dynamics in this region are of global relevance. Microbial structure is an important driver of decomposition and is affected by both abiotic and biotic conditions. Yet how activities of soil-dwelling organisms in higher trophic levels influence microbial structure and function is unclear. In this study, we demonstrate that predicted changes in abundances of a dominant predator and warming interactively affect the structure of litter-dwelling fungal communities in the Arctic. These findings suggest predators may have widespread, indirect cascading effects on microbial communities, which could influence ecosystem responses to future climate change.
Complete genome of a multiresistant
Pasteurella multocida
isolated from a diseased Austrian bovine calf
Authors: Barta Leonard , Prieto Beatriz Daza , Stöger Anna , Polzer Daniel , Schmoll Friedrich , Sattler Tatjana , Ruppitsch Werner
Published: June 2024

Pasteurella multocida
(
P. multocida
) plays an important role in bovine respiratory diseases. Here we describe the complete genome of a multiresistant
P. multocida
. The strain was isolated from the lung of a diseased, 4-month old, male Fleckvieh calf with a clinical history of bronchopneumonia in Upper Austria.
Supplemental Material: Heat pulse–dominated magmatic storage: The 33 ka dacite dome eruption at Taápaca volcano (Central Andes)
Authors: Rout Smruti Sourav , al. et
Published: June 2024

 Text S1, data sets S2–S5, and materials S6 and S7.  
Study of the Method of Photon Neutralization of Powerful Beams of Negative Ions at the Budker Institute of Nuclear Physics
Authors: Popov S. S. , Atlukhanov M. G. , Burdakov A. V. , Ivanov A. A. , Kurkuchekov V. V. , Sanin A. L. , Skovorodin D. I. , Trunev Yu. A. , Shikhovtsev I. V.
Published: June 2024

A short review of the studies carried out at the Budker Institute of Nuclear Physics, Siberian Branch, Russian Academy of Sciences (BINP SB RAS) on the photon neutralization of the beams of negative ions is presented. The principal distinctive feature of the presented approach consists in the nonresonant accumulation of photons in a limited space. Their confinement is based on the adiabatic motion of photons in a system of concave mirrors, which is insensitive to the quality of the injected radiation. An analysis is carried out of the possibility of using the neutralizer based on such a nonresonant photon trap in large-scale installations such as ITER and TRT, and a future experiment is described on the photon neutralization using a beam of negative hydrogen ions with energy up to 130 keV and a current of about 10 mA.