bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential Candidate Genes Associated with Litter Size in Goats: A Review
Authors: Chen Wenting , Han Ying , Chen Yinghui , Liu Xiaotong , Liang Huili , Wang Changfa , Khan Muhammad Zahoor
Published: January 2025

This review examines genetic markers associated with litter size in goats, a key reproductive trait impacting productivity in small ruminant farming. Goats play a vital socioeconomic role in both low- and high-income regions; however, their productivity remains limited due to low reproductive efficiency. Litter size, influenced by multiple genes and environmental factors, directly affects farm profitability and sustainability by increasing the output per breeding cycle. Recent advancements in genetic research have identified key genes and pathways associated with reproductive traits, including gonadotropin-releasing hormone (GnRH), inhibin (INHAA), Kit ligand (KITLG), protein phosphatase 3 catalytic subunit alpha (PPP3CA), prolactin receptor (PRLR), POU domain class 1 transcription factor 1 (POU1F1), anti-Müllerian hormone (AMH), bone morphogenetic proteins (BMP), growth differentiation factor 9 (GDF9), and KISS1 and suppressor of mothers against decapentaplegic (SMAD) family genes, among others. These genes regulate crucial physiological processes such as folliculogenesis, hormone synthesis, and ovulation. Genome-wide association studies (GWASs) and transcriptomic analyses have pinpointed specific genes linked to increased litter size, highlighting their potential in selective breeding programs. By incorporating genomic data, breeding strategies can achieve higher selection accuracy, accelerate genetic gains, and improve reproductive efficiency. This review emphasizes the importance of genetic markers in optimizing litter size and promoting sustainable productivity in goat farming.
Melatonin in Male Dromedary Camel (Camelus dromedarius) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes
Authors: Doghbri Lamia , Carvajal-Serna Melissa , Atigui Moufida , Casao Adriana , Peña-Delgado Victoria , Seddik Mabrouk-Mouldi , Dbara Mohamed , Pérez-Pé Rosaura , Hammadi Mohamed
Published: January 2025

Camels (Camelus dromedarius) are seasonal short-day breeders, regulated by photoperiod and melatonin secretion. However, no studies have explored melatonin levels in camel seminal plasma or their relationship with testosterone, age, or climatic factors, nor is it known whether melatonin receptors exist in camel spermatozoa to respond to seminal melatonin. This study aimed to analyze melatonin levels in camel seminal plasma and its specific receptors in spermatozoa. Semen samples were obtained from November to March (breeding season). Testosterone and melatonin levels were measured in seminal plasma by ELISA. Melatonin receptors were localized in spermatozoa using immunofluorescence, and their presence was confirmed by Western Blot. Melatonin levels were higher from November to January and decreased in February and March. No correlation between testosterone and melatonin levels was found, but both hormones were negatively correlated with daylength (p = 0.0089 and p = 0.0688, respectively). Testosterone, but not melatonin, levels were affected by age. Two melatonin receptors (MT1, MT2) were detected on camel spermatozoa, with several immunotypes labeled mainly in the tail and post-acrosome region, but also in the acrosome and neck. Western Blot analysis confirmed the presence of these receptors, showing a 39 kDa band for MT1 and a 36 kDa band for MT2. Understanding melatonin’s effects on sperm could help ejaculates’ processing procedures, semen handling, and infertility issues in camels.
Effectiveness of Juvenile Eriocheir sinensis in Controlling Pomacea canaliculata and Their Growth and Nutritional Response to Feeding on the Snail
Authors: Wang Jie , Zhang Yixiang , Lv He , Shen Weiqi , Fang Weiping , Zhang Rongfei , Zhao Hanqu , Sheng Qiang
Published: January 2025

In recent years, Pomacea canaliculata has aggressively invaded rice fields in Asia, resulting in significant agricultural losses. Biological control can effectively reduce the damage caused by P. canaliculata. This research evaluates E. sinensis as a biocontrol for P. canaliculata, focusing on its feeding preferences and optimal control density on snails of three sizes, as well as the effects on the nutritional quality of juvenile crabs post consumption. Our findings reveal that juvenile E. sinensis exhibit a strong preference for feeding on small snails, effectively managing populations at densities of 600 snails per tank. Crab feeding significantly reduces the survival and activity of snails. Furthermore, consumption of P. canaliculata meat alters the crabs’ physiology. Female crabs show elevated levels of molting hormones, total energy yield (TEY), and condition factor (CF), while males demonstrate increased lipid, moisture, and TEY levels. The amino acid profiles shift, with higher isoleucine and leucine levels in female hepatopancreas and reduced histidine in the muscles. Notably, the total Σn-3 PUFA content in female muscles fed on snail meat exceeds that of those given commodity grain. This study underscores the dual benefits of employing juvenile E. sinensis for managing P. canaliculata while enhancing crab farming practices.
Comparison of Performance, Egg Quality, and Egg Cost of Different Laying Genotypes in Free-Range System from 21 to 44 Weeks of Age
Authors: Aygun Ali , Narinç Doğan , Arısoy Hasan
Published: January 2025

The aim of this study was to compare the performance, egg quality and economic aspects of laying hybrids of different genotypes in free-range system. In the study, three different laying genotypes (Lohmann Brown, Lohmann Sandy and ATAK-S genotype) were used. Each group consisted of four replicates and each replicate contained 20 hens. Egg production, broken–cracked egg ratio, egg weight, feed consumption, feed efficiency, and viability were determined as performance traits. Eggshell strength, Haugh unit, yolk color, and shell color were determined as egg quality traits. The costs of eggs were assessed for economic analysis. The Lohmann Sandy genotype produced more eggs than the Lohmann Brown genotype (p < 0.05). The ATAK-S genotype reached 50% egg production age sooner than other genotypes (p < 0.05). The Lohmann Sandy and Lohmann Brown genotypes produced fewer broken eggs and heavier eggs compared to the ATAK-S genotype (p < 0.05). The ATAK-S genotype consumed more feed than the Lohmann Sandy and Lohmann Brown genotypes (p < 0.05). The Lohmann Sandy and Lohmann Brown genotypes have better feed efficiency compared to the ATAK-S genotype (p < 0.05). Eggs from the Lohmann Sandy genotype exhibit greater resistance to breakage compared to those from other genotypes (p < 0.05). The highest quality of albumen was identified in the eggs of the Lohmann Brown genotype (p < 0.05). The yolk color of Lohmann Brown genotype eggs is darker than those of other genotypes (p < 0.05). The shell color of Lohmann genotype eggs is a darker brown compared to eggs of other genotypes (p < 0.05). The cheapest egg cost was determined from eggs produced from the Lohmann Sandy genotype (p < 0.05). According to the results obtained from our study, it would be more appropriate to prefer the Lohmann Sandy genotype in the free-range system.
Comparative Analysis of miRNA Expression Profiles of Yak Milk-Derived Exosomes at Different Altitudes
Authors: Ren Wenwen , La Yongfu , Ma Xiaoming , Wu Xiaoyun , Guo Xian , Chu Min , Yan Ping , Lan Xianyong , Liang Chunnian
Published: January 2025

Yaks are a rare and unique animal species inhabiting the Qinghai–Tibet Plateau; they are renowned for their remarkable ability to thrive in harsh environments. Milk-derived exosomes, tiny vesicles containing various biological molecules, play crucial roles in numerous pathological and physiological processes, including cell growth, development, and immune regulation. This study delved into the microRNA expression profiles of yak milk-derived exosomes collected from both high- and low-altitude populations using small RNA sequencing. These miRNAs were found to be implicated in pathways associated with mammary gland inflammation, virus infection regulation, and heat stress response. Functional enrichment analyses, utilizing GO and KEGG databases, revealed that the target genes of these differentially expressed miRNAs are enriched in signaling pathways crucial for Th17 cell differentiation and the Ras-MAPK signaling pathway. In conclusion, this research illuminates the adaptive mechanisms of yaks through the differential expression of miRNAs in their milk-derived exosomes across varying environmental conditions. These findings provide a valuable foundation for future investigations into yak resilience and the potential of milk-derived exosomes as tools for disease management and immune modulation.
Predictive Modeling and Experimental Analysis of Cyclic Shear Behavior in Sand–Fly Ash Mixtures
Authors: Yıldız Özgür , Fırat Çabalar Ali
Published: January 2025

This study presents a comprehensive investigation into the cyclic shear behavior of sand–fly ash mixtures through experimental and data-driven modeling approaches. Cyclic direct shear tests were conducted on mixtures containing fly ash at 0%, 2.5%, 5%, 10%, 15%, and 20% by weight to examine the influence of fly ash content on the shear behavior under cyclic loading conditions. The tests were carried out under a constant stress of 100 kPa to simulate field-relevant stress conditions. Results revealed that the fly ash content initially reduces shear strength at lower additive contents, but shear strength increases and reaches a maximum at 20% fly ash content. The findings highlight the trade-offs in mechanical behavior associated with varying fly ash proportions. To enhance the understanding of cyclic shear behavior, a Nonlinear Autoregressive Model with External Input (NARX) model was employed. Using data from the loading cycles as input, the NARX model was trained to predict the final shear response under cyclic conditions. The model demonstrated exceptional predictive performance, achieving a coefficient of determination (R2) of 0.99, showcasing its robustness in forecasting the cyclic shear performance based on the composition of the mixtures. The insights derived from this research underscore the potential of incorporating fly ash in sand mixtures for soil stabilization in geotechnical engineering. Furthermore, the integration of advanced machine learning techniques such as NARX models offers a powerful tool for predicting the behavior of soil mixtures, facilitating more effective and data-driven decision-making in geotechnical applications. Evidently, this study not only advances the understanding of cyclic shear behavior in fly ash–sand mixtures but also provides a framework for employing data-driven methodologies to address complex geotechnical challenges.
Bioimpedance Spectra Confirm Breast Cancer Cell Secretome Induces Early Changes in the Cytoskeleton and Migration of Mesenchymal Stem Cells
Authors: Sánchez-Corrales Ana Laura , González-Díaz César Antonio , Calzada-Mendoza Claudia Camelia , Arrieta-Valencia Jesús , Sánchez-Mendoza María Elena , Amaya-Espinoza Juan Luis , Gutiérrez-Iglesias Gisela
Published: January 2025

Mesenchymal stem cell (MSC) treatments take advantage of the ability of these cells to migrate to target sites, although they have been shown to move in response to tumor influence. Currently, tools are being developed to detect these opportune changes in cellular behavior patterns. No reports of such changes in the morphological patterns or migration of MSCs in the presence of a tumor environment, which would provide information of high diagnostic value, have been made. We determined the changes in the cytoskeleton and migration of MSCs exposed to the secretome of breast tumor cells via bioimpedance records. MSCs were cultured and incubated in the presence of 24 and 48 h secretomes of the MCF-7 tumor cell line. The proliferation, migration, morphology, cytoskeleton, and electrical bioimpedance were evaluated at 48 h for cells treated with 24 and 48 h secretomes. Secretomes induced early morphological changes related to the migration of MSCs, directly confirmed via bioimpedance, but no changes in cell proliferation were found. These changes cannot be related to a transformation or malignancy phenotype. The modification of the bioimpedance patterns recorded from the first hours suggests that this method can be applied in an innovative way to detect early changes in a cellular population in the clinical diagnostic setting.
Efficiency Optimization of the Main Operating Points of an EV Traction Motor
Authors: Lee Gi-haeng , You Yong-min
Published: January 2025

Motor efficiency presents a trade-off between low-speed and high-speed regions. Additionally, the cross-sectional area of hairpin motors employing rectangular wires is larger than that of round wires, thereby amplifying AC copper losses. As the operating speed increases, the AC copper loss also becomes more pronounced; therefore, efficiently determining the optimal design point considering these characteristics is essential. This study optimizes the efficiency of an electric vehicle (EV) simulation is conducted using MATLAB 2024, and the main operating points according to the driving cycle are selected. For the EV simulation to select the main operating points, the driving cycle of the multi-cycle test method, which is used for measuring domestic driving range, is considered to enhance the validity of the operating points. The efficiency optimization of the main operating points was performed considering the AC copper loss, and essential parameters such as the torque ripple and total harmonic distortion of the back-electromotive force were incorporated as constraints. Furthermore, the predictive performances of the 11 metamodels were compared to identify the most suitable metamodel for the output and design variables. Subsequently, the selected metamodel was integrated with four optimization algorithms to optimize the design.
Exploring the Mechanism of Clostridium autoethanogenum Protein for Broiler Growth Based on Gut Microbiota and Serum Metabolomics
Authors: Shan Chunqiao , Liu Yan , Liu Sisi , Li Chuang , Ma Chaoxin , Yu Hongmin , Li Juan , Jiang Guotuo , Tian Jing
Published: January 2025

Intestinal health is vital for poultry production, and protein plays a key role in intestinal nutrition. The present study used 16S rRNA gene sequencing and serum metabolomics to investigate the effect of CAP on the cecal microflora structure and serum metabolites in 42-day-old broiler chickens. A total of 480 one-day-old Arbor Acres broiler chickens were randomly divided into four treatments with twelve replicates comprising 10 chickens each, evenly divided by sex. The four groups were basal diet group (CAP0), treatment group 1 (CAP2), treatment group 2 (CAP3), and treatment group 3 (CAP4). The broilers in the CAP0 group were fed a basal diet (without CAP), while those in the CAP2, CAP3, and CAP4 groups received diets containing 2%, 3%, and 4% CAP, respectively. Growth performance results showed that dietary CAP supplementation significantly ameliorated the feed conversion rate (FCR) of broilers at 42 days in the CAP3 and CAP4 groups (p < 0.05). Microbial results revealed that CAP did not alter the dominant microorganisms in the cecum at the phylum, family, and genus levels. LEfSe analysis showed significantly higher relative abundances of p_Desulfobacterota, f_Desulfovibrionaceae, and g_Ruminococcus in the CAP3 group compared to the CAP0 and CAP4 groups. Metabolomic analyses indicated that the effect of incorporating CAP into the diet on serum metabolites primarily focused on organic acids and their derivatives, small peptides, amino acid derivatives, and oxidized lipids. The addition of 3% or 4% CAP to the diet can enhance metabolic pathways such as the citrate cycle (TCA cycle) and arginine and proline metabolism. In summary, incorporating CAP into the diet can increase the relative abundance of beneficial bacteria in the cecum and improve the feed conversion efficiency of broilers by enhancing amino acid and energy metabolism.
Complete Mitochondrial Genome of Niphon spinosus (Perciformes: Niphonidae): Genome Characterization and Phylogenetic Analysis
Authors: Patil Maheshkumar Prakash , Kim Jong-Oh , Yoo Seung Hyun , Shin Jiyoung , Yang Ji-Young , Kim Kyunghoi , Kim Gun-Do
Published: January 2025

The species Niphon spinosus (Cuvier, 1829) is the only representative of the family Niphonidae and the genus Niphon, and its taxonomic history is complicated; it is still unclear in a phylogenetic sense. In this study, we report the complete mitochondrial genome of N. spinosus (OP391482), which was determined to be 16,503 bp long with biased A + T contents (53.8%) using next-generation technology. The typical set of 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and one control region (D-loop) are included in the mitochondrial genome. The H-strand encoded 28 genes (14 tRNA, 2 rRNA, and 12 PCGs), and D-loop, whereas the L-strand encoded the remaining 9 genes (8 tRNA and ND6). Its nucleotide composition, gene arrangement, codon usage patterns, and tRNA secondary structures are identical with other members of the Percoidei suborder. Furthermore, we reconstructed phylogenetic trees based on the 13 PCGs. The resulting phylogenetic trees showed N. spinosus placing as a separate lineage within the family Niphonidae, its close relationship to Trachinus draco (Trachinidae), and the clustering of major subfamilies like Luciopercinae and Percinae of the Percoidei suborder. These findings will contribute to future studies on the evolutionary history, population genetics, molecular taxonomy, and phylogeny of N. spinosus and related species.
Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma
Authors: Alizadeh Bahmani Amir Hossein , Abdel-Aziz Mahmoud I. , Hashimoto Simone , Bang Corinna , Brandstetter Susanne , Corcuera-Elosegui Paula , Franke Andre , Gorenjak Mario , Harner Susanne , Kheiroddin Parastoo , López-Fernández Leyre , Neerincx Anne H. , Pino-Yanes Maria , Potočnik Uroš , Sardón-Prado Olaia , Toncheva Antoaneta A. , Wolff Christine , Kabesch Michael , Kraneveld Aletta D. , Vijverberg Susanne J. H. , Maitland-van der Zee Anke H. ,   on behalf of the SysPharmPediA consortium
Published: January 2025

Background/Objectives: Metered-dose inhalers (MDIs) and dry powder inhalers (DPIs) are common inhaled corticosteroid (ICS) inhaler devices. The difference in formulation and administration technique of these devices may influence oral cavity microbiota composition. We aimed to compare the saliva microbiome in children with moderate-to-severe asthma using ICS via MDIs versus DPIs. Methods: Saliva samples collected from 143 children (6–17 yrs) with moderate-to-severe asthma across four European countries (The Netherlands, Germany, Spain, and Slovenia) as part of the SysPharmPediA cohort were subjected to 16S rRNA sequencing. The microbiome was compared using global diversity (α and β) between two groups of participants based on inhaler devices (MDI (n = 77) and DPI (n = 65)), and differential abundance was compared using the Analysis of Compositions of Microbiomes with the Bias Correction (ANCOM-BC) method. Results: No significant difference was observed in α-diversity between the two groups. However, β-diversity analysis revealed significant differences between groups using both Bray–Curtis and weighted UniFrac methods (adjusted p-value = 0.015 and 0.044, respectively). Significant differential abundance between groups, with higher relative abundance in the MDI group compared to the DPI group, was detected at the family level [Carnobacteriaceae (adjusted p = 0.033)] and at the genus level [Granulicatella (adjusted p = 0.021) and Aggregatibacter (adjusted p = 0.011)]. Conclusions: Types of ICS devices are associated with different saliva microbiome compositions in moderate-to-severe pediatric asthma. The causal relation between inhaler types and changes in saliva microbiota composition needs to be further evaluated, as well as whether this leads to different potential adverse effects in terms of occurrence and level of severity.
Unveiling the Potency of Gardenia Extract Against H. pylori: Insights from In Vitro and In Vivo Studies
Authors: Werawatganone Pornpen , Werawatganon Duangporn , Noonak Nattida , Chayanupatkul Maneerat , Chatsuwan Tanittha , Klaikeaw Naruemon , Muangsiri Walaisiri , Siriviriyakul Prasong
Published: January 2025

Background and aim: Gardenia jasminoides (G. jasminoides) could treat various inflammatory diseases. This study aimed to investigate the effects of G. jasminoides fruit extract on gastric inflammation and protective mechanisms in Helicobacter pylori (H. pylori)-induced gastritis. Experimental procedure: G. jasminoides fruit extract was prepared and analyzed for geniposide content. The inhibitory effect of the extract on H. pylori growth was investigated using the disk diffusion method. The in vitro anti-inflammatory property of the extract was evaluated using the erythrocyte membrane stabilization method. Thirty-five male Sprague–Dawley rats were inoculated with H. pylori (108–1010 colony-forming unit/mL) and divided into five groups. Each group was treated with various doses of the extract (98–395 mg/kg). The serum and stomach tissue of the rats were evaluated using enzyme-linked immunosorbent assay, histopathology, and immunohistochemistry. Results and conclusions: The geniposide content in the dried extract was 8.12% ± 0.79% by dry weight. The inhibition zone was observed at the extract ≥ 1.97 mg/disk, and the extract presented anti-inflammatory potential. The H. pylori-inoculated rats had a significant increase in serum interleukin (IL)-17, IL-33, and gastric epidermal growth factor (EGF) levels and a significant decrease in serum prostaglandin E2 level (p < 0.05) in conjunction with the development of gastric inflammation on histopathology. The treatment of the extract could significantly decrease the serum IL-17, IL-33, and gastric EGF levels, significantly increase the serum PGE2 level (p < 0.05), and improve gastric histopathology. Thus, G. jasminoides fruit extract attenuated H. pylori-induced gastritis by inhibiting bacterial growth, reducing inflammation, and enhancing protective mechanisms.
A Computational Model of Hybrid Trunk-like Robots for Synergy Formation in Anticipation of Physical Interaction
Authors: Morasso Pietro
Published: January 2025

Trunk-like robots have attracted a lot of attention in the community of researchers interested in the general field of bio-inspired soft robotics, because trunk-like soft arms may offer high dexterity and adaptability very similar to elephants and potentially quite superior to traditional articulated manipulators. In view of the practical applications, the integration of a soft hydrostatic segment with a hard-articulated segment, i.e., a hybrid kinematic structure similar to the elephant’s body, is probably the best design framework. It is proposed that this integration should occur at the conceptual/cognitive level before being implemented in specific soft technologies, including the related control paradigms. The proposed modeling approach is based on the passive motion paradigm (PMP), originally conceived for addressing the degrees of freedom problem of highly redundant, articulated structures. It is shown that this approach can be naturally extended from highly redundant to hyper-redundant structures, including hybrid structures that include a hard and a soft component. The PMP model is force-based, not motion-based, and it is characterized by two main computational modules: the Jacobian matrix of the hybrid kinematic chain and a compliance matrix that maps generalized force fields into coordinated gestures of the whole-body model. It is shown how the modulation of the compliance matrix can be used for the synergy formation process, which coordinates the hyper-redundant nature of the hybrid body model and, at the same time, for the preparation of the trunk tip in view of a stable physical interaction of the body with the environment, in agreement with the general impedance–control concept.
A Helicopter Survey for Cliff-Nesting Raptors Along the Dalton Highway in Northern Alaska, 2010
Authors: Craig Erica H. , Herriges Jim D. , Craig Tim H.
Published: January 2025

We conducted a helicopter survey in 2010 for cliff-nesting raptors along the Dalton Highway in northern Alaska. The study area extended from the Yukon River northward ~395 km through the Brooks Range to the Arctic Plain. We documented 55 occupied raptor nesting territories, including 25 Golden Eagle (Aquila chrysaetos), 11 Gyrfalcon (Falco rusticolus), and 10 Peregrine Falcon (Falco peregrinus) territories. We also recorded vacant stick nests and raptor perch sites on cliffs. We identified more occupied eagle territories and hundreds more vacant eagle stick nests than were previously known in the study area. The average number of Golden Eagle nestlings/nesting pairs at the time of the survey was 1.5 ± 0.6. The most productive Golden Eagle pairs were located in the northern part of the study area. The number of occupied eagle territories in 2010 is far fewer than the number of eagle stick nests we recorded. Our data indicate that the distribution of nesting eagles in the 2010 study area is different than in the past. Whether this reflects changes in eagle density; a response to availability of prey, climate change, human activities, or a combination of these; or some other factor warrants further investigation. Intensive surveys along the Dalton Highway in the 1970s found no occupied nests for Peregrine Falcons or Gyrfalcons but we found 10 and 11, respectively. Therefore, the Peregrine Falcon and Gyrfalcon nests we recorded in 2010 reflect an increase in the number of known nesting pairs there.
Examining the Effects of Different Types of Achievement Goal Orientation on Undergraduate Students’ Engagement in Distance Learning: The Mediating Effect of Self-Efficacy
Authors: Han Tingzhi , Xu Guoxing , Lu Wenli
Published: January 2025

This study investigates the impact and mechanisms of achievement goal orientation on online learning engagement among undergraduates, using a sample of 461 students enrolled in online courses from four universities of varying levels in Shanghai, China. Self-efficacy is introduced as a mediating variable, and structural equation modeling is employed to assess the effects. The findings reveal that different types of achievement goals have varying natures and intensities in their associations with undergraduate online learning engagement. Self-efficacy partially mediates the effect of mastery-approach goals on online learning engagement, but does not mediate the effects of mastery-avoidance, performance-approach, or performance-avoidance goals. The study recommends leveraging the guiding role of mastery-approach achievement goals to help students set scientific and reasonable goals, and stimulating students’ self-efficacy through the dual influences of external support and internal motivation. With the support of these strategies, significant improvements in undergraduate online learning engagement can be achieved.
Simulation of Fire Emergency Evacuation in a Primary School Based on Pathfinder Software
Authors: Safari Mahdi , Afkhami Reza , Amerzadeh Mohammad , Zaroushani Vida
Published: January 2025

(1) Background: In Iran, burn injuries are the second leading cause of death among children, surpassed only by traffic accidents. This study aims to simulate fire emergency evacuations in an elementary school using Pathfinder software, focusing on identifying architectural factors that influence evacuation efficiency. Children are particularly vulnerable in emergencies due to their limited understanding of danger and tendency to panic, making the development of effective evacuation strategies essential for their safety. (2) Methods: We analyzed the emergency evacuation of 522 occupants at a selected elementary school in Qazvin City, Iran. Using Pathfinder 2021 software, we examined various evacuation scenarios, including evacuation density, traffic patterns on different routes, and flow at exits. We calculated the Required Safe Egress Time (RSET) and Available Safe Egress Time (ASET) from the simulation data. Data collection involved a comprehensive assessment of the school’s geometric characteristics, as well as the demographic and anthropometric profiles of the occupants. (3) Results: The simulations revealed a total evacuation time of 386 s, with an ASET of 180 s. The average passenger flow rate was only 1.35 persons per second, indicating a slow evacuation process. Our findings highlighted that specific architectural features, such as classroom size and door dimensions, significantly affect evacuation times. Additionally, when simulating an evacuation with 170 fewer students, the total evacuation time decreased by 128 s, suggesting that adhering to recommended class size standards can enhance evacuation efficiency. Notably, the RSET consistently exceeded the ASET (180 s) across all simulations. (4) Conclusions: This research deepens our understanding of school evacuation dynamics and underscores the need for improved architectural designs and safety protocols to protect vulnerable populations, especially children. Future studies should focus on implementing targeted interventions based on these findings to mitigate risks associated with school fires. Additionally, the results indicate that installing automatic fire alarms and extinguishing systems, along with conducting regular emergency evacuation drills for students, could significantly reduce RSET.
A Review of the Application of Hemispherical Photography in Urban Outdoor Thermal Comfort Studies
Authors: Sima Lei , Liu Yisha , Shang Xiaowei , Yuan Qi , Zhang Yunming
Published: January 2025

Thermal comfort studies are paramount in enhancing future urban living conditions, and hemispherical photography has emerged as a widely employed field measurement technique in outdoor thermal comfort research. This comprehensive review systematically analyzed 142 outdoor thermal comfort studies conducted over the past decade using hemispherical photography methods, revealing that its primary application lies in objectively describing environmental information and constructing associated indices. In contrast, the number of studies focusing on subjectively assessing environmental factors remains relatively low; however, it is rapidly increasing due to its demonstrated effectiveness and convenience compared to other methodologies within this domain. Overall, despite certain limitations, such as higher labor costs and limited temporal/spatial coverage when describing environmental information, hemispherical photography still retains its advantage of providing accurate data acquisition for outdoor thermal comfort research. In recent years, advancements in mobile measurement tools and techniques have enhanced the richness and versatility of acquired information while leveraging the image specificity inherent to hemispherical photography, which continues to play a pivotal role in subjective assessments related to human perception of outdoor thermal comfort.
Effect of Gut Dysbiosis on Onset of GI Cancers
Authors: Kumari Seema , Srilatha Mundla , Nagaraju Ganji Purnachandra
Published: January 2025

Dysbiosis in the gut microbiota plays a significant role in GI cancer development by influencing immune function and disrupting metabolic functions. Dysbiosis can drive carcinogenesis through pathways like immune dysregulation and the release of carcinogenic metabolites, and altered metabolism, genetic instability, and pro-inflammatory signalling, contributing to GI cancer initiation and progression. Helicobacter pylori infection and genotoxins released from dysbiosis, lifestyle and dietary habits are other factors that contribute to GI cancer development. Emerging diagnostic and therapeutic approaches show promise in colorectal cancer treatment, including the multitarget faecal immunochemical test (mtFIT), standard FIT, and faecal microbiota transplantation (FMT) combined with PD-1 inhibitors. We used search engine databases like PubMed, Scopus, and Web of Science. This review discusses the role of dysbiosis in GI cancer onset and explores strategies such as FMT, probiotics, and prebiotics to enhance the immune response and improve cancer therapy outcomes.
Single-Nucleus RNA Sequencing Reveals Cellular Transcriptome Features at Different Growth Stages in Porcine Skeletal Muscle
Authors: Chen Ziyu , Wu Xiaoqian , Zheng Dongbin , Wang Yuling , Chai Jie , Zhang Tinghuan , Wu Pingxian , Wei Minghong , Zhou Ting , Long Keren , Li Mingzhou , Jin Long , Chen Li
Published: January 2025

Porcine latissimus dorsi muscle (LDM) is a crucial source of pork products. Meat quality indicators, such as the proportion of muscle fibers and intramuscular fat (IMF) deposition, vary during the growth and development of pigs. Numerous studies have highlighted the heterogeneous nature of skeletal muscle, with phenotypic differences reflecting variations in cellular composition and transcriptional profiles. This study investigates the cellular-level transcriptional characteristics of LDM in large white pigs at two growth stages (170 days vs. 245 days) using single-nucleus RNA sequencing (snRNA-seq). We identified 56,072 cells across 12 clusters, including myofibers, fibro/adipogenic progenitor (FAP) cells, muscle satellite cells (MUSCs), and other resident cell types. The same cell types were present in the LDM at both growth stages, but their proportions and states differed. A higher proportion of FAPs was observed in the skeletal muscle of 245-day-old pigs. Additionally, these cells exhibited more active communication with other cell types compared to 170-day-old pigs. For instance, more interactions were found between FAPs and pericytes or endothelial cells in 245-day-old pigs, including collagen and integrin family signaling. Three subclasses of FAPs was identified, comprising FAPs_COL3A1+, FAPs_PDE4D+, and FAPs_EBF1+, while adipocytes were categorized into Ad_PDE4D+ and Ad_DGAT2+ subclasses. The proportions of these subclasses differed between the two age groups. We also constructed differentiation trajectories for FAPs and adipocytes, revealing that FAPs in 245-day-old pigs differentiated more toward fibrosis, a characteristic reminiscent of the high prevalence of skeletal muscle fibrosis in aging humans. Furthermore, the Ad_PDE4D+ adipocyte subclass, predominant in 245-day-old pigs, originated from FAPs_PDE4D+ expressing the same gene, while the Ad_DGAT2+ subclass stemmed from FAPs_EBF1+. In conclusion, our study elucidates transcriptional differences in skeletal muscle between two growth stages of pigs and provides insights into mechanisms relevant to pork meat quality and skeletal muscle diseases.
Glutamate- and Tartrate-Based Inhibitor Films to Prevent Chloride-Induced Corrosion in Reinforced Concrete: Efficiency of Dry or Hydrated Films via Molecular Dynamics Simulations
Authors: Raffaini Giuseppina , Catauro Michelina , Bolzoni Fabio , Ormellese Marco
Published: January 2025

Due to their barrier effect against chloride penetration, corrosion inhibitors are widely used to prevent chloride-induced corrosion in reinforced concrete structures. The mechanisms of interaction between the protective film on carbon steel and chloride ions represent a crucial aspect of the design of new inhibitors. Theoretical studies based on molecular mechanic (MM) and molecular dynamic (MD) methods have proven useful in research on the formation of passive films and their intermolecular interactions with chloride ions, both under dry and hydrated conditions, allowing for further comparison with experimental data. In this study, glutamate- and tartrate-based inhibitor films are investigated. After MM/MD simulations, chloride ions are found to be kept away from the dry protective films via electrostatic repulsion, hence remaining distant from the lepidocrocite γ-FeO(OH) surface. These coatings are able to efficiently prevent chloride adsorption and film penetration due to the presence of COO− groups, which electrostatically and dynamically repel chlorides over time. Even on hydrated coatings, when complete coverage of the solid surface occurs, these OCIs are good potential candidates for preventing chloride-induced corrosion; however, in the presence of water, the penetration of chloride ions across the protective films can occur, thus rendering them relatively less effective.
Long-Haul COVID: Investigating the Effects Within the Mauritian Context
Authors: Soreefan Aïsha B. , Putteeraj Manish , Somanah Jhoti
Published: January 2025

Background: COVID-19 infection can have a protracted course in many survivors, with varied sociodemographic and medical characteristics, exhibiting a plethora of symptoms that have consequential impacts on their quality of life. This study sought to gather pertinent data about the prevalence of Long-Haul COVID (LC), the predisposing factors to this condition and the burden on the quality of life of Mauritian survivors. Research Setting: A cross-sectional study was performed using an adapted online questionnaire, using two definitions of Long COVID, namely the WHO and NICE, SIGN and RCGP definitions. Associations between LC and categorical variables were employed to explore relationships between LC and ratio (FAS, FSS, PCS-12, MCS-12) variables. Simple and multivariable logistic regression models were used to assess the predictors and outcomes associated with LC. Findings: Of 285 Mauritians with a confirmed history of COVID-19 infection, 64.2% developed Long COVID (WHO LC-38.9%, NICE, SIGN and RCGP LC-55.8%). The most prevalent symptoms were fatigue or muscle weakness (88.0%), cough (57.4%), difficulty concentrating (55.2%), trouble remembering or memorising (49.7%), insomnia or sleep disturbance (43.7%), amongst others. Statistically significant associations were determined between LC and age, gender, vaccination status, severity of acute illness, reinfections, self-perception of disease and having more than five acute symptoms. Long COVID positively correlated with fatigue. Both Long COVID and severe fatigue (F = 73.266, p < 0.001) negatively impacted PCS-12. Fatigue had no significant impact on MCS-12. Conclusions: This study demonstrated the presence of Long COVID in the Mauritian population. Long COVID manifests as a complex and long-lasting affliction that affects even young adults with disabling outcomes, owing to multiple lingering symptoms but, most importantly, fatigue. The latter brings about distressing declines in physical and overall quality of life that thump both individual and societal health and productivity.
Study on the Influence of Different Feeding Habitats on the Behavioral Habits of Siberian Cranes in the Songnen Plain
Authors: Zhu Shiying , Deng Guangyi , Jiang Haibo , Gao Jie , He Chunguang , Zhang Yan , Cao Yingyue
Published: January 2025

As a habitat for waterbirds, wetlands are key to their survival, reproduction and development. Waterbirds usually prefer breeding, wintering and resting in fixed locations. Siberian cranes (Grus leucogeranus), which are highly dependent on wetlands, have long fed on farmland at migratory stopover sites. To explore the reason for this phenomenon, the time budgets of Siberian crane populations stopping over on farmland or in wetland habitats were studied and compared in this study. The results showed that the farmlands visited by the Siberian cranes are rich in food resources and have experienced low levels of disturbance. The temporal distribution of feeding behavior on farmland (53.50%) was greater than that in wetland habitats (31.96%). The variations in warning, flying and walking behavior on farmland were less than those in wetlands. The feeding efficiency on farmland was significantly greater than that in wetlands. Therefore, Siberian cranes transiting the Songnen Plain leave wetland habitats and stop over on farmland, representing a behavior that occurs more than just occasionally. Instead, they change their foraging habitat choices based on the optimal foraging theory. As a transit feeding area for Siberian cranes, farmland poses a significant risk, and the restoration of wetland habitats and food resources is still needed. This study can provide theoretical support for the conservation of rare and endangered species (the Siberian crane) and the management of stopover sites.
Epilepsy Diagnosis from EEG Signals Using Continuous Wavelet Transform-Based Depthwise Convolutional Neural Network Model
Authors: Dişli Fırat , Gedikpınar Mehmet , Fırat Hüseyin , Şengür Abdulkadir , Güldemir Hanifi , Koundal Deepika
Published: January 2025

Background/Objectives: Epilepsy is a prevalent neurological disorder characterized by seizures that significantly impact individuals and their social environments. Given the unpredictable nature of epileptic seizures, developing automated epilepsy diagnosis systems is increasingly important. Epilepsy diagnosis traditionally relies on analyzing EEG signals, with recent deep learning methods gaining prominence due to their ability to bypass manual feature extraction. Methods: This study proposes a continuous wavelet transform-based depthwise convolutional neural network (DCNN) for epilepsy diagnosis. The 35-channel EEG signals were transformed into 35-channel images using continuous wavelet transform. These images were then concatenated horizontally and vertically into a single image (seven rows by five columns) using Python’s PIL library, which served as input for training the DCNN model. Results: The proposed model achieved impressive performance metrics on unseen test data: 95.99% accuracy, 94.27% sensitivity, 97.29% specificity, and 96.34% precision. Comparative analyses with previous studies and state-of-the-art models demonstrated the superior performance of the DCNN model and image concatenation technique. Conclusions: Unlike earlier works, this approach did not employ additional classifiers or feature selection algorithms. The developed model and image concatenation method offer a novel methodology for epilepsy diagnosis that can be extended to different datasets, potentially providing a valuable tool to support neurologists globally.
Risk Factor Analysis in Vascular Access Complications for Hemodialysis Patients
Authors: Roşu Cristian Dan , Bolintineanu Sorin Lucian , Căpăstraru Bogdan Florin , Iacob Roxana , Stoicescu Emil Robert , Petrea Claudia Elena
Published: January 2025

Background: Chronic kidney disease (CKD) and renal failure remain critical global health challenges, with vascular access complications posing significant obstacles in hemodialysis management. Methods: This study investigates the early and late complications associated with vascular access procedures in a cohort of 1334 patients from Timiș County Emergency Clinical Hospital. Patients were categorized into early complications, occurring within 30 days postoperatively, and late complications, developing beyond this period. Demographic data, comorbidities, and lifestyle factors, including age, gender, body mass index (BMI), smoking status, hypertension, diabetes, and cardiovascular disease (CVD), were recorded and analyzed. Early complications included thrombosis, hemorrhage, edema, and non-maturation, while late complications involved thrombosis, aneurysmal dilation, venous hypertension, and infections. Results: Hemorrhage (32.3%) and thrombosis (30.8%) were the most prevalent early complications, influenced significantly by diabetes and hypertension. Non-maturation showed a strong association with diabetes and cardiovascular disease (odds ratio: 1.70). For late complications, thrombosis was most frequent, with hypertensive patients exhibiting increased risk (relative risk: 1.18). BMI was a significant factor in both early and late complications. Risk analysis using odds ratios and relative risks revealed distinct patterns of complication risks based on comorbidities and smoking status. Logistic regression modeling for thrombosis demonstrated moderate predictive accuracy (AUC: 0.64). Conclusions: These findings suggest that clinical interventions, such as stricter perioperative glycemic and blood pressure control, and personalized surgical strategies for patients with high BMI or comorbidities, could significantly reduce the incidence of vascular access complications and improve outcomes in this high-risk population.
Impact of Collagenated and Non-Collagenated Deproteinized Bovine Bone Mineral on Schneiderian Membrane Integrity in Rabbits
Authors: Yamada Rihito , Xavier Samuel Porfirio , Nakajima Yasushi , Silva Erick Ricardo , Botticelli Daniele , Teranishi Yuki , Baba Shunsuke
Published: January 2025

Background: This investigation focused on the influence of collagen on the integrity of the Schneiderian membrane during maxillary sinus augmentation in a rabbit model. The aim of this study was to elucidate the relationship between membrane integrity and bone regeneration in augmented maxillary sinuses using collagenated and non-collagenated grafts, through detailed histological and histomorphometric analyses. Methods: In this forward-looking, randomized, split-mouth design, bilateral maxillary sinus augmentation was conducted on 12 rabbits. One sinus was filled with deproteinized bovine bone material (DBBM) as the grafting material (non-collagenated; control), while a combination of DBBM particles integrated with 10% porcine-derived type I collagen was used in the contralateral sinus (collagenated; test). Histological analyses were carried out following healing periods of 2 and 12 weeks. Results: At the 2-week time point, six sites of thinned mucosa (<40 µm) and no perforations were observed in the collagenated group, while twenty-one thinned mucosa sites (p = 0.027 between test and control) and two perforations (p = 0.175 between test and control) were found in the non-collagenated group. After 12 weeks of healing, the number of sites with thinned mucosa was 20 in the collagenated group and 19 in the non-collagenated group, with four perforations observed in each group. These perforations were distributed across three sinuses in the collagenated group and two sinuses in the non-collagenated group. No statistically significant differences were found between the groups. Conclusions: The addition of 10% collagen to deproteinized bovine bone mineral initially provided protection against mucosal thinning and perforation after 2 weeks of healing. However, by week 12, this protective effect diminished, resulting in similar rates of mucosal thinning and perforations in both groups.