bluuebunny/crossref_metadata_2025 huggingface dataset:
Virulence and pathogenicity determinants in whole genome sequence of Fusarium udum causing wilt of pigeon pea
Authors: Srivastava Alok K. , Srivastava Ruchi , Yadav Jagriti , Singh Alok K. , Tiwari Praveen K. , Srivastava Anchal K. , Sahu Pramod K. , Singh Shiv M. , Kashyap Prem Lal
Published: February 2023

The present study deals with whole genome analysis of Fusarium udum, a wilt causing pathogen of pigeon pea. The de novo assembly identified a total of 16,179 protein-coding genes, of which 11,892 genes (73.50%) were annotated using BlastP and 8,928 genes (55.18%) from KOG annotation. In addition, 5,134 unique InterPro domains were detected in the annotated genes. Apart from this, we also analyzed genome sequence for key pathogenic genes involved in virulence, and identified 1,060 genes (6.55%) as virulence genes as per the PHI-BASE database. The secretome profiling of these virulence genes indicated the presence of 1,439 secretory proteins. Of those, an annotation of 506 predicted secretory proteins through CAZyme database indicated maximum abundance of Glycosyl hydrolase (GH, 45%) family proteins followed by auxiliary activity (AA) family proteins. Interestingly, the presence of effectors for cell wall degradation, pectin degradation, and host cell death was found. The genome comprised approximately 895,132 bp of repetitive elements, which includes 128 long terminal repeats (LTRs), and 4,921 simple sequence repeats (SSRs) of 80,875 bp length. The comparative mining of effector genes among different Fusarium species revealed five common and two specific effectors in F. udum that are related to host cell death. Furthermore, wet lab experiment validated the presence of effector genes like SIX (for Secreted in Xylem). We conclude that deciphering the whole genome of F. udum would be instrumental in understanding evolution, virulence determinants, host-pathogen interaction, possible control strategies, ecological behavior, and many other complexities of the pathogen.
Increasing power generation to a single-chamber compost soil urea fuel cell for carbon-neutral bioelectricity generation: A novel approach
Authors: Magotra Verjesh Kumar , Lee Dong-Jin , Kim D. Y. , Lee S. J. , Kang T. W. , Magotra Arjun , Inamdar Akbar I. , Shrestha Nabeen K. , Patil Supriya A. , Thammanu Siriluck , Jeon Hee Chang
Published: February 2023

Microbial fuel cells (CS-UFC) utilize waste resources containing biodegradable materials that play an essential role in green energy. MFC technology generates “carbon-neutral” bioelectricity and involves a multidisciplinary approach to microbiology. MFCs will play an important role in the harvesting of “green electricity.” In this study, a single-chamber urea fuel cell is fabricated that uses these different wastewaters as fuel to generate power. Soil has been used to generate electrical power in microbial fuel cells and exhibited several potential applications to optimize the device; the urea fuel concentration is varied from 0.1 to 0.5 g/mL in a single-chamber compost soil urea fuel cell (CS-UFC). The proposed CS-UFC has a high power density and is suitable for cleaning chemical waste, such as urea, as it generates power by consuming urea-rich waste as fuel. The CS-UFC generates 12 times higher power than conventional fuel cells and exhibits size-dependent behavior. The power generation increases with a shift from the coin cell toward the bulk size. The power density of the CS-UFC is 55.26 mW/m2. This result confirmed that urea fuel significantly affects the power generation of single-chamber CS-UFC. This study aimed to reveal the effect of soil properties on the generated electric power from soil processes using waste, such as urea, urine, and industrial-rich wastewater as fuel. The proposed system is suitable for cleaning chemical waste; moreover, the proposed CS-UFC is a novel, sustainable, cheap, and eco-friendly design system for soil-based bulk-type design for large-scale urea fuel cell applications.
Endogenous relapse and exogenous reinfection in recurrent pulmonary tuberculosis: A retrospective study revealed by whole genome sequencing
Authors: He Wencong , Tan Yunhong , Song Zexuan , Liu Binbin , Wang Yiting , He Ping , Xia Hui , Huang Fei , Liu Chunfa , Zheng Huiwen , Pei Shaojun , Liu Dongxin , Ma Aijing , Cao Xiaolong , Zhao Bing , Ou Xichao , Wang Shengfen , Zhao Yanlin
Published: February 2023

Background

Tuberculosis may reoccur due to reinfection or relapse after initially successful treatment. Distinguishing the cause of TB recurrence is crucial to guide TB control and treatment. This study aimed to investigate the source of TB recurrence and risk factors related to relapse in Hunan province, a high TB burden region in southern China.

Methods

A population-based retrospective study was conducted on all culture-positive TB cases in Hunan province, China from 2013 to 2020. Phenotypic drug susceptibility testing and whole-genome sequencing were used to detect drug resistance and distinguish between relapse and reinfection. Pearson chi-square test and Fisher exact test were applied to compare differences in categorical variables between relapse and reinfection. The Kaplan–Meier curve was generated in R studio (4.0.4) to describe and compare the time to recurrence between different groups. p < 0.05 was considered statistically significant.

Results

Of 36 recurrent events, 27 (75.0%, 27/36) paired isolates were caused by relapse, and reinfection accounted for 25.0% (9/36) of recurrent cases. No significant difference in characteristics was observed between relapse and reinfection (all p > 0.05). In addition, TB relapse occurs earlier in patients of Tu ethnicity compared to patients of Han ethnicity (p < 0.0001), whereas no significant differences in the time interval to relapse were noted in other groups. Moreover, 83.3% (30/36) of TB recurrence occurred within 3 years. Overall, these recurrent TB isolates were predominantly pan-susceptible strains (71.0%, 49/69), followed by DR-TB (17.4%, 12/69) and MDR-TB (11.6%, 8/69), with mutations mainly in codon 450 of the rpoB gene and codon 315 of the katG gene. 11.1% (3/27) of relapse cases had acquired new resistance during treatment, with fluoroquinolone resistance occurring most frequently (7.4%, 2/27), both with mutations in codon 94 of gyrA.

Conclusion

Endogenous relapse is the main mechanism leading to TB recurrences in Hunan province. Given that TB recurrences can occur more than 4 years after treatment completion, it is necessary to extend the post-treatment follow-up period to achieve better management of TB patients. Moreover, the relatively high frequency of fluoroquinolone resistance in the second episode of relapse suggests that fluoroquinolones should be used with caution when treating TB cases with relapse, preferably guided by DST results.
Microbial colonization and degradation of marine microplastics in the plastisphere: A review
Authors: Zhai Xinyi , Zhang Xiao-Hua , Yu Min
Published: February 2023

Marine microplastic pollution is a growing problem for ecotoxicology that needs to be resolved. In particular, microplastics may be carriers of “dangerous hitchhikers,” pathogenic microorganisms, i.e., Vibrio. Microplastics are colonized by bacteria, fungi, viruses, archaea, algae and protozoans, resulting in the biofilm referred to as the “plastisphere.” The microbial community composition of the plastisphere differs significantly from those of surrounding environments. Early dominant pioneer communities of the plastisphere belong to primary producers, including diatoms, cyanobacteria, green algae and bacterial members of the Gammaproteobacteria and Alphaproteobacteria. With time, the plastisphere mature, and the diversity of microbial communities increases quickly to include more abundant Bacteroidetes and Alphaproteobacteria than natural biofilms. Factors driving the plastisphere composition include environmental conditions and polymers, with the former having a much larger influence on the microbial community composition than polymers. Microorganisms of the plastisphere may play key roles in degradation of plastic in the oceans. Up to now, many bacterial species, especially Bacillus and Pseudomonas as well as some polyethylene degrading biocatalysts, have been shown to be capable of degrading microplastics. However, more relevant enzymes and metabolisms need to be identified. Here, we elucidate the potential roles of quorum sensing on the plastic research for the first time. Quorum sensing may well become a new research area to understand the plastisphere and promote microplastics degradation in the ocean.
Colletotrichum gloeosporioides Cg2LysM contributed to virulence toward rubber tree through affecting invasive structure and inhibiting chitin-triggered plant immunity
Authors: Zhao Li , Liao Zhiwen , Feng Liping , An Bang , He Chaozu , Wang Qiannan , Luo Hongli
Published: February 2023

Fungal chitin, as a typical microorganism-associated molecular pattern (PAMP), was recognized by plant LysM-containing protein to induce immunity called pattern-triggered immunity (PTI). To successfully infect host plant, fungal pathogens secreted LysM-containing effectors to inhibit chitin-induced plant immunity. Filamentous fungus Colletotrichum gloeosporioides caused rubber tree anthracnose which resulted in serious loss of natural rubber production worldwide. However, little is known about the pathogenesis mediated by LysM effector of C. gloeosporioide. In this study, we identified a two LysM-containing effector in C. gloeosporioide and named as Cg2LysM. Cg2LysM was involved not only in conidiation, appressorium formation, invasion growth and the virulence to rubber tree, but also in melanin synthesis of C. gloeosporioides. Moreover, Cg2LysM showed chitin-binding activity and suppression of chitin-triggered immunity of rubber tree such as ROS production and the expression of defense relative genes HbPR1, HbPR5, HbNPR1 and HbPAD4. This work suggested that Cg2LysM effector facilitate infection of C. gloeosporioides to rubber tree through affecting invasive structure and inhibiting chitin-triggered plant immunity.
The most common European HINT1 neuropathy variant phenotype and its case studies
Authors: Rozevska Marija , Rots Dmitrijs , Gailite Linda , Linde Ronalds , Mironovs Stanislavs , Timcenko Maksims , Linovs Viktors , Locmele Dzintra , Micule Ieva , Lace Baiba , Kenina Viktorija
Published: February 2023

HINT1 is an ubiquitous homodimeric purine phosphoramidase belonging to the histidine-triad superfamily. In neurons, HINT1 stabilizes the interaction of different receptors and regulates the effects of their signaling disturbances. Changes in HINT1 gene are associated with autosomal recessive axonal neuropathy with neuromyotonia. Aim of the study was detailed description of patients' phenotype with HINT1 homozygous NM_005340.7: c.110G>C (p.Arg37Pro) variant. Seven homozygous and three compound heterozygous patients were recruited and evaluated using standardized tests for CMT patients, in four patients' nerve ultrasonography was performed. The median age of symptom onset was 10 years (range 1–20), with initial complaints being distal lower limb weakness with gait impairment, combined with muscle stiffness, more pronounced in the hands than in the legs and worsened by cold. Arm muscles became involved later, presenting with distal weakness and hypotrophy. Neuromyotonia was present in all reported patients and is thus a diagnostic hallmark. Electrophysiological studies demonstrated axonal polyneuropathy. Impaired mental performance was observed in six out of ten cases. In all patients with HINT1 neuropathy, ultrasound examination showed significantly reduced muscle volume as well as spontaneous fasciculations and fibrillations. The nerve cross-sectional areas of the median and ulnar nerves were closer to the lower limits of the normal values. None of the investigated nerves had structural changes. Our findings broaden the phenotype of HINT1-neuropathy and have implications for diagnostics and ultrasonographic evaluation of HINT1-neuropathy patients.
Application of EEG in migraine
Authors: Zhang Ning , Pan Yonghui , Chen Qihui , Zhai Qingling , Liu Ni , Huang Yanan , Sun Tingting , Lin Yake , He Linyuan , Hou Yue , Yu Qijun , Li Hongyan , Chen Shijiao
Published: February 2023

Migraine is a common disease of the nervous system that seriously affects the quality of life of patients and constitutes a growing global health crisis. However, many limitations and challenges exist in migraine research, including the unclear etiology and the lack of specific biomarkers for diagnosis and treatment. Electroencephalography (EEG) is a neurophysiological technique for measuring brain activity. With the updating of data processing and analysis methods in recent years, EEG offers the possibility to explore altered brain functional patterns and brain network characteristics of migraines in depth. In this paper, we provide an overview of the methodology that can be applied to EEG data processing and analysis and a narrative review of EEG-based migraine-related research. To better understand the neural changes of migraine or to provide a new idea for the clinical diagnosis and treatment of migraine in the future, we discussed the study of EEG and evoked potential in migraine, compared the relevant research methods, and put forwards suggestions for future migraine EEG studies.
A novel method for repeated cerebrospinal fluid sampling and long-term monitoring of intracranial pressure in rats
Authors: Hao Guangshan , Zhang Qia , Chen Weiyu , Mo Jun
Published: February 2023

Cannulation implantation into the cisterna magna is an important procedure in cerebrospinal fluid (CSF) sampling and intracranial pressure (ICP) monitoring. The disadvantages of existing techniques include the risk of brain damage, compromised muscle mobility, and the complexity of the procedures. In the present study, the authors describe a modified, simple, and reliable procedure for long-term cannulation implantation into the cisterna magna in rats. The device consists of four parts: the puncture segment, the connection segment, the fixing segment, and the external segment. Intraoperative ICP monitoring and post-operative computed tomography (CT) scans were performed, which confirmed the accuracy and safety of this method. There were no limitations on the daily activities of the rats when long-term drainage was carried out for 1 week. This new technique offers an improved method of cannulation and will be a potentially useful method for CSF sampling and ICP monitoring in neuroscience research.
Nutrition in the prevention and treatment of endometriosis: A review
Authors: Barnard Neal D. , Holtz Danielle N. , Schmidt Natalie , Kolipaka Sinjana , Hata Ellen , Sutton Macy , Znayenko-Miller Tatiana , Hazen Nicholas D. , Cobb Christie , Kahleova Hana
Published: February 2023

Endometriosis is characterized by the presence of endometrial tissues outside the uterine lining, typically on the external surface of the uterus, the ovaries, fallopian tubes, abdominal wall, or intestines. The prevalence of endometriosis in North America, Australia, and Europe is ~1–5% in women of reproductive age. Treatment options for endometriosis are limited. While over-the-counter medications may be used to reduce acute pain, hormonal treatments are common and may interfere with fertility. In more severe cases, laparoscopic excision procedures and even hysterectomies are used to treat the pain associated with endometriosis. Nutritional interventions may be helpful in the prevention and treatment of endometriosis and associated pain. Reducing dietary fat and increasing dietary fiber have been shown to reduce circulating estrogen concentrations, suggesting a potential benefit for individuals with endometriosis, as it is an estrogen-dependent disease. Meat consumption is associated with greater risk of developing endometriosis. Anti-inflammatory properties of plant-based diets may benefit women with endometriosis. Additionally, seaweed holds estrogen-modulating properties that have benefitted postmenopausal women and offers potential to reduce estradiol concentrations in pre-menopausal women. Furthermore, consumption of vitamin D has been shown to reduce endometrial pain via increased antioxidant capacity and supplementation with vitamins C and E significantly reduced endometriosis symptoms, compared with placebo. More randomized clinical trials are needed to elucidate the role of diet in endometriosis.
Extraction, physicochemical properties, and antioxidant activity of natural melanin from Auricularia heimuer fermentation
Authors: Ma Yinpeng , Zhang Piqi , Dai Xiaodong , Yao Xiuge , Zhou Shuyang , Ma Qingfang , Liu Jianing , Tian Shuang , Zhu Jianan , Zhang Jiechi , Kong Xianghui , Bao Yihong
Published: February 2023

Introduction

Natural melanin from Auricularia heimuer have numerous beneficial biological properties, which were used as a safe and healthy colorant in several industries.

Methods

In this study, single-factor experiments, Box-Behnken design (BBD), and response surface methodology (RSM) were employed to investigate the effects of alkali-soluble pH, acid precipitation pH, and microwave time on the extraction yield of Auricularia heimuer melanin (AHM) from fermentation. Ultraviolet-visible spectrum (UV-Vis), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), and high-performance liquid chromatography (HPLC) were used to analyze the extracted AHM. The solubility, stability, and antioxidant activities of AHM were also measured.

Results

The results showed that alkali-soluble pH, acid precipitation pH, and microwave time significantly affected the AHM yield, with the following optimized microwave-assisted extraction conditions: alkali-soluble pH of 12.3, acid precipitation pH of 3.1, and microwave time of 53 min, resulting in an AHM extraction yield of 0.4042%. AHM exhibited a strong absorption at 210 nm, similar to melanin from other sources. FT-IR spectroscopy also revealed that AHM exhibited the three characteristic absorption peaks of natural melanin. The HPLC chromatogram profile of AHM showed a single symmetrical elution peak with a 2.435 min retention time. AHM was highly soluble in alkali solution, insoluble in distilled water and organic solvents, and demonstrated strong DPPH, OH, and ABTS free radical scavenging activities.

Discussion

This study provides technical support to optimize AHM extraction for use in the medical and food industries.
TIGIT signaling and its influence on T cell metabolism and immune cell function in the tumor microenvironment
Authors: Jantz-Naeem Nouria , Böttcher-Loschinski Romy , Borucki Katrin , Mitchell-Flack Marisa , Böttcher Martin , Schraven Burkhart , Mougiakakos Dimitrios , Kahlfuss Sascha
Published: February 2023

One of the key challenges for successful cancer therapy is the capacity of tumors to evade immune surveillance. Tumor immune evasion can be accomplished through the induction of T cell exhaustion via the activation of various immune checkpoint molecules. The most prominent examples of immune checkpoints are PD-1 and CTLA-4. Meanwhile, several other immune checkpoint molecules have since been identified. One of these is the T cell immunoglobulin and ITIM domain (TIGIT), which was first described in 2009. Interestingly, many studies have established a synergistic reciprocity between TIGIT and PD-1. TIGIT has also been described to interfere with the energy metabolism of T cells and thereby affect adaptive anti-tumor immunity. In this context, recent studies have reported a link between TIGIT and the hypoxia-inducible factor 1-α (HIF1-α), a master transcription factor sensing hypoxia in several tissues including tumors that among others regulates the expression of metabolically relevant genes. Furthermore, distinct cancer types were shown to inhibit glucose uptake and effector function by inducing TIGIT expression in CD8+ T cells, resulting in an impaired anti-tumor immunity. In addition, TIGIT was associated with adenosine receptor signaling in T cells and the kynurenine pathway in tumor cells, both altering the tumor microenvironment and T cell-mediated immunity against tumors. Here, we review the most recent literature on the reciprocal interaction of TIGIT and T cell metabolism and specifically how TIGIT affects anti-tumor immunity. We believe understanding this interaction may pave the way for improved immunotherapy to treat cancer.
Targeting fat mass and obesity-associated protein mitigates human colorectal cancer growth in vitro and in a murine model
Authors: Phan Thuy , Nguyen Vu H. , Su Rui , Li Yangchan , Qing Ying , Qin Hanjun , Cho Hyejin , Jiang Lei , Wu Xiwei , Chen Jianjun , Fakih Marwan , Diamond Don J. , Goel Ajay , Melstrom Laleh G.
Published: February 2023

Introduction

Colorectal cancer (CRC) remains a significant cause of cancer related mortality. Fat mass and obesity-associated protein (FTO) is a m6A mRNA demethylase that plays an oncogenic role in various malignancies. In this study we evaluated the role of FTO in CRC tumorigenesis.

Methods

Cell proliferation assays were conducted in 6 CRC cell lines with the FTO inhibitor CS1 (50-3200 nM) (± 5-FU 5-80 mM) and after lentivirus mediated FTO knockdown. Cell cycle and apoptosis assays were conducted in HCT116 cells (24 h and 48 h, 290 nM CS1). Western blot and m6A dot plot assays were performed to assess CS1 inhibition of cell cycle proteins and FTO demethylase activity. Migration and invasion assays of shFTO cells and CS1 treated cells were performed. An in vivo heterotopic model of HCT116 cells treated with CS1 or with FTO knockdown cells was performed. RNA-seq was performed on shFTO cells to assess which molecular and metabolic pathways were impacted. RT-PCR was conducted on select genes down-regulated by FTO knockdown.

Results

We found that the FTO inhibitor, CS1 suppressed CRC cell proliferation in 6 colorectal cancer cell lines and in the 5-Fluorouracil resistant cell line (HCT116-5FUR). CS1 induced cell cycle arrest in the G2/M phase by down regulation of CDC25C and promoted apoptosis of HCT116 cells. CS1 suppressed in vivo tumor growth in the HCT116 heterotopic model (p< 0.05). Lentivirus knockdown of FTO in HCT116 cells (shFTO) mitigated in vivo tumor proliferation and in vitro demethylase activity, cell growth, migration and invasion compared to shScr controls (p< 0.01). RNA-seq of shFTO cells compared to shScr demonstrated down-regulation of pathways related to oxidative phosphorylation, MYC and Akt/ mTOR signaling pathways.

Discussion

Further work exploring the targeted pathways will elucidate precise downstream mechanisms that can potentially translate these findings to clinical trials.
Increased nuchal translucency in children with congenital heart defects and normal karyotype—is there a correlation with mortality?
Authors: Kristensen Rasmus , Omann Camilla , Gaynor J. William , Rode Line , Ekelund Charlotte K. , Hjortdal Vibeke E.
Published: February 2023

Objectives

Our objective was to investigate if an increased nuchal translucency (NT) was associated with higher mortality in chromosomally normal children with congenital heart defects (CHD).

Methods

In a nationwide cohort using population-based registers, we identified 5,633 liveborn children in Denmark with a pre- or postnatal diagnosis of CHD from 2008 to 2018 (incidence of CHD 0.7%). Children with chromosomal abnormalities and non-singletons were excluded. The final cohort compromised 4,469 children. An increased NT was defined as NT > 95th-centile. Children with a NT > 95th-centile vs. NT < 95th-centile including subgroups of simple- and complex CHD were compared. Mortality was defined as death from natural causes, and mortalities were compared among groups. Survival analysis with Cox-regression was used to compare rates of mortality. Analyses were adjusted for mediators (possibly explanatory factors between increased NT and higher mortality): preeclampsia, preterm birth and small for gestational age. And for confounding effects of extracardiac anomalies and cardiac intervention, due to their close association to both the exposure and the outcome (i.e., confounders).

Results

Of the 4,469 children with CHD, 754 (17%) had complex CHD and 3,715 (83%) simple CHD. In the combined group of CHDs the mortality rate was not increased when comparing those with a NT > 95th-centile to those with a NT < 95th-centile [Hazard ratio (HR) 1.6, 95%CI 0.8;3.4, p = 0.2]. In simple CHD there was a significantly higher mortality rate with a HR of 3.2 (95%CI: 1.1;9.2, p = 0.03) when having a NT > 95th centile. Complex CHD had no differences in mortality rate between a NT > 95th-centile and NT < 95th-centile (HR 1.1, 95%CI: 0.4;3.2, p = 0.8). All analysis adjusted for severity of CHD, cardiac operation and extracardiac anomalies. Due to limited numbers the association to mortality for a NT > 99th centile (>3.5 mm) could not be assessed. Adjustment for mediating (preeclampsia, preterm birth, small for gestational age) and confounding variables (extracardiac anomalies, cardiac intervention) did not alter the associations significantly, except for extracardiac anomalies in simple CHD.

Conclusion

An increased NT > 95th-centile is correlated with higher mortality in children with simple CHD, but the underlying cause is unknown and undetected abnormal genetics might explain the correlation rather than the increased NT itself, hence further research is warranted.
A qualitative content analysis of the experience of hypospadias care: The importance of owning your own narrative
Authors: Phillips Lottie , Dennermalm Nicklas , Örtqvist Lisa , Engberg Hedvig , Holmdahl Gundela , Fossum Magdalena , Möller Anders , Nordenskjöld Agneta
Published: February 2023

Objectives

There is a lack of studies on men's individual experiences of living with hypospadias. We aimed to explore the personal experiences of having hypospadias in relation to healthcare and surgery.

Subjects and methods

Purposive sampling was used to include men (aged 18 and over) with hypospadias representing different phenotypes (from distal to proximal) and ages in order to maximise the variation and richness of our data. Seventeen informants, aged 20–49, were included in the study. In-depth semi-structured interviews were conducted between 2019 and 2021. Inductive qualitative content analysis was used to analyse the data.

Results

We identified three categories: (1) Having surgery, which comprised the decision to operate, the experience of having surgery, and the outcomes of surgery; (2) Going to the doctor, which focused on follow-up care, re-entering care in adolescence or adulthood, and the experience of healthcare interactions; (3) Being informed, both about hypospadias in general, as well as about your specific body and medical history. There was overall a large variation in experiences. The latent theme across the data was the importance of owning your own narrative.

Conclusion

The experience of men with hypospadias in healthcare is complex and varied, highlighting the difficulty of fully standardised care. Based on our results, we suggest that follow-up should be offered in adolescence, and that ways of accessing care for late onset complications be made clear. We further suggest clearer consideration for the psychological and sexual aspects of hypospadias. Consent and integrity in all aspects and all ages of hypospadias care should be adapted to the maturity of the individual. Access to trustworthy information is key, both directly from educated healthcare staff and if possible, from websites or patient-led forums. Healthcare can play a key role in providing the growing individual with tools to understand and address concerns that may develop relating to their hypospadias through life, giving them ownership over their own narrative.
Kimura disease in children: A report of 11 cases and review of the literature
Authors: Mai Yumiao , Wang Yingjie , Sun Pan , Jing Zhaohe , Dong Pengpeng , Liu Jian
Published: February 2023

Introduction

Kimura disease (KD), also known as eosinophilic hyperplastic lymphoid granuloma, is a rare benign chronic inflammatory condition, which is featured with the painless progressive mass located in the subcutaneous area of the head and neck region, elevated peripheral blood eosinophils, and raised serum immunoglobulin E (IgE) levels. KD is uncommon in clinical practice, especially with low incidence in children, so it often leads to misdiagnosis or missed diagnosis in pediatric patients.

Methods

The clinical data of 11 pediatric patients with KD in the authors' institution were retrospectively analyzed.

Results

There were 11 pediatric patients with KD enrolled in total, including 9 male patients and 2 female patients (sex ratio 4.5:1). The median age at diagnosis stood at 14 years (range 5-18 years), the initial symptoms in all patients included painless subcutaneous masses and focal swelling, the duration of symptoms ranged from 1 month to 10 years, and the average duration was 24.45 months. Six patients had single lesions, and 5 had multiple lesions. The highest proportion of lesion regions were parotid gland (n = 5, 31.3%) and retroauricular (n = 5, 31.3%), followed by cervical lymph nodes (n = 4, 25%), and others (n = 2,12.5; elbow n = 1; back n = 1). The eosinophil absolute count elevated in all patients, ranging from 0.71×109/L to 10.35 ×109/L (normal range 0.02-0.52×109/L). IgE levels were increased in all 7 patients who underwent serum immunoglobulin examination (normal range <100 IU/mL). Three patients received oral corticosteroid treatment while 2 relapsed. Three patients received surgical resection combined with oral corticosteroid treatment, and no patient relapsed. The other 3 patients received surgery and radiotherapy, surgery combined with corticosteroid and cyclosporin and corticosteroid combined with leflunomide respectively, and no patient relapsed.

Conclusion

Base on the study, it is found that Kimura disease is rare and may have the atypical symptoms in pediatric patients, combination therapy is recommended to reduce recurrence, and long-term follow-up should be performed.
Succession of a natural desert vegetation community after long-term fencing at the edge of a desert oasis in northwest China
Authors: Zhang Yan , Wang Guohua , Gou Qianqian , Zhang Yu , Liu Jing , Gao Min
Published: February 2023

Fencing is the most economical method of restoring degraded desert ecosystems, and plays an important role in promoting plant community diversity and productivity, as well as stable ecosystem structure and function. In this study, we selected a typical degraded desert plant community (Reaumuria songorica–Nitraria tangutorum) on the edge of a desert oasis in the Hexi Corridor in northwest China. We then investigated succession in this plant community and corresponding changes in soil physical and chemical characteristics over 10 years of fencing restoration to analyze the mutual feedback mechanisms. The results showed that: 1) The diversity of plant species in the community increased significantly over the study period, especially the number of herbaceous layer species, which increased from four in the early stage to seven in the late stage. The dominant species also changed, with the dominant shrub layer species shifting from N. sphaerocarpa in the early stage to R. songarica in the late stage. The dominant herbaceous layer species changed from the annual herb Suaeda glauca in the early stage to S. glauca and Artemisia scoparia in the middle stage, and ultimately to A. scoparia and Halogeton arachnoideus in the late stage. In the late stage, Zygophyllum mucronatum, H. arachnoideus, and Eragrostis minor began to invade, and the density of perennial herbs also increased significantly (from 0.01 m-2 to 0.17 m-2 for Z. kansuense in year seven). 2) As the duration of fencing increased, the soil organic matter (SOM) and total nitrogen (TN) contents first decreased then increased, whereas the available nitrogen, potassium, and phosphorus contents showed the opposite trend. 3) Changes in community diversity were mainly affected by the nursing effects of the shrub layer, as well as soil physical and chemical properties. That is, fencing significantly increased the vegetation density of the shrub layer, which promoted growth and development of the herbaceous layer. However, community species diversity was positively correlated with SOM and TN. The diversity of the shrub layer was positively correlated with the water content of deep soil, whereas that of the herbaceous layer was positively correlated with SOM, TN, and soil pH. The SOM content in the later stage of fencing was 1.1 times that in the early stage of fencing. Thus, fencing restored the density of the dominant shrub species and significantly increased species diversity, especially in the herb layer. Studying plant community succession and soil environmental factors under long-term fencing restoration is highly significant for understanding community vegetation restoration and ecological environment reconstruction at the edge of desert oases.
A method of cotton root segmentation based on edge devices
Authors: Yu Qiushi , Tang Hui , Zhu Lingxiao , Zhang Wenjie , Liu Liantao , Wang Nan
Published: February 2023

The root is an important organ for plants to absorb water and nutrients. In situ root research method is an intuitive method to explore root phenotype and its change dynamics. At present, in situ root research, roots can be accurately extracted from in situ root images, but there are still problems such as low analysis efficiency, high acquisition cost, and difficult deployment of image acquisition devices outdoors. Therefore, this study designed a precise extraction method of in situ roots based on semantic segmentation model and edge device deployment. It initially proposes two data expansion methods, pixel by pixel and equal proportion, expand 100 original images to 1600 and 53193 respectively. It then presents an improved DeeplabV3+ root segmentation model based on CBAM and ASPP in series is designed, and the segmentation accuracy is 93.01%. The root phenotype parameters were verified through the Rhizo Vision Explorers platform, and the root length error was 0.669%, and the root diameter error was 1.003%. It afterwards designs a time-saving Fast prediction strategy. Compared with the Normal prediction strategy, the time consumption is reduced by 22.71% on GPU and 36.85% in raspberry pie. It ultimately deploys the model to Raspberry Pie, realizing the low-cost and portable root image acquisition and segmentation, which is conducive to outdoor deployment. In addition, the cost accounting is only $247. It takes 8 hours to perform image acquisition and segmentation tasks, and the power consumption is as low as 0.051kWh. In conclusion, the method proposed in this study has good performance in model accuracy, economic cost, energy consumption, etc. This paper realizes low-cost and high-precision segmentation of in-situ root based on edge equipment, which provides new insights for high-throughput field research and application of in-situ root.
Drought priming mechanisms in wheat elucidated by in-situ determination of dynamic stomatal behavior
Authors: Yang Mengxiang , He Jiawei , Sun Zhuangzhuang , Li Qing , Cai Jian , Zhou Qin , Wollenweber Bernd , Jiang Dong , Wang Xiao
Published: February 2023

Stomata play a critical role in balancing photosynthesis and transpiration, which are essential processes for plant growth, especially in response to abiotic stress. Drought priming has been shown to improve drought tolerance. Lots of studies have been done with the response of stomatal behavior to drought stress. However, how the stomatal dynamic movement in intact wheat plants response to drought priming process is not known. Here, a portable microscope was used to take microphotographs in order to in-stiu determination of stomatal behavior. Non-invasive micro-test technology was used for measurements of guard cell K+, H+ and Ca2+ fluxes. Surprisingly, the results found that primed plants close stomatal much faster under drought stress, and reopening the stomatal much quicker under recovery, in relation to non-primed plants. Compared with non-primed plants, primed plants showed higher accumulation of ABA and Ca2+ influx rate in guard cells under drought stress. Furthermore, genes encoding anion channels were higher expressed and K+ outward channels activated, leading to enhanced K+ efflux, resulting in faster stomatal closure in primed plants than non-primed plants. During recovery, both guard cell ABA and Ca2+ influx of primed plants were found to be significantly reducing K+ efflux and accelerating stomatal reopening. Collectively, a portable non-invasive stomatal observation of wheat found that priming promoted faster stomatal closure under drought stress and faster reopening during post-drought recovery in relation to non-primed plants, thereby enhancing overall drought tolerance.
Examining the challenge-hindrance-threat distinction of job demands alongside job resources
Authors: Fernandez de Henestrosa Martha , Sischka Philipp E. , Steffgen Georges
Published: February 2023

Purpose

Previous research conducted on the Job Demands-Resources model has mostly ignored the newly introduced Challenge-Hindrance-Threat distinction of workplace stressors. Thus, to better understand the nature of job demands, the present study aimed to explore this distinction of job demands within the framework of the Job Demands-Resources model. Moreover, it examined competing theoretical frameworks by investigating the associations between job characteristics and psychological health variables (i.e., burnout, vigor).

Design/methodology/approach

Data were collected via computer assisted telephone interview among a representative sample of employees working in Luxembourg (n = 1,506).

Findings

Structural Equation Modeling supported the distinctiveness of the proposed demand categories in terms of their effects. The health impairing nature of threats, hindrances, and challenges, as well as the motivational potential of resources was supported. Yet, scarce support was found for the moderating effects of demands and resources on employees’ well-being.

Research implications

Based on these findings, we argue for an extended framework of job characteristics, which will more accurately describe their nature and effects on employees.

Practical implications

In order to promote employee’s well-being, occupational health advisors need to be aware of the distinct demand-wellbeing relations when implementing job redesign measures.

Originality/value

Combining multiple theoretical frameworks is considered a leading principle in occupational health research. The present study implements an extended classification framework of workplace stressors into one of today’s most influential theoretical framework of job characteristics.
Socially priming dogs in an overimitation task
Authors: Mackie Louise , Huber Ludwig
Published: February 2023

Overimitation — the copying of another’s unnecessary or irrelevant actions toward a goal — is largely considered to be uniquely human. Recent studies, however, have found evidence of this behavior in dogs. Humans seem to overimitate more or less depending on social factors, such as the cultural origin of the demonstrator. Like humans, dogs may have social motivations behind their overimitation, since they have been shown to copy irrelevant actions more from their caregivers than from strangers. By using priming methodology, this study aimed to investigate whether dogs’ overimitation can be facilitated via the experimental manipulation of their attachment-based motivations. To test this, we invited caregivers to demonstrate goal-irrelevant and relevant actions to their dog, following either a dog-caregiver relationship prime, a dog-caregiver attention prime, or no prime. Our results showed no significant main effect of priming on copying behavior for either relevant or irrelevant actions, but we found a trend that unprimed dogs copied the least actions overall. Additionally, dogs copied their caregiver’s relevant actions more often and more faithfully as the number of trials increased. Our final finding was that dogs were much more likely to copy irrelevant actions after (rather than before) already achieving the goal. This study discusses the social motivations behind dog imitative behavior, and has potential methodological implications regarding the influence of priming on dog behavioral studies.
Life and mental health in limbo of the Ukraine war: How can helpers assist civilians, asylum seekers and refugees affected by the war?
Authors: Anjum Gulnaz , Aziz Mudassar , Hamid Hadar Khasrow
Published: February 2023

The terror spread by the war disrupts lives and severs families, leaving individuals and communities devastated. People are left to fend for themselves on multiple levels, especially psychologically. It is well documented that war adversely affects non-combatant civilians, both physically and psychologically. However, how the war puts civilians’ lives in a limbo is an under-researched area. This paper focuses on three aspects: (1) how the mental health and well-being of Ukrainian civilians, asylum seekers, and refugees are affected by the war caused limbo; (2) what factors affect this process of being stuck in the limbo of war; and (3) how psychologists and helpers in the war-ridden and host countries can provide meaningful support. Based on the authors’ own practical work with Ukrainian civilians, refugees, and professional helpers during the war, this paper provides an overview of multi-level factors that impact human psyches in a war, and possible ways to help those who are living in the war limbo. In this research and experiential learning-based review, we offer some helpful strategies, action plans, and resources for the helpers including psychologists, counselors, volunteers, and relief workers. We emphasize that the effects of war are neither linear nor equal for all civilians and refugees. Some will recover and return to a routine life while others will experience panic attacks, trauma, depression, and even PTSD, which can also surface much later and can prolong over the years. Hence, we provide experience-based ways of dealing with short-term and prolonged trauma of living with war and post-traumatic stress disorder (PTSD). Mental health professionals and other helpers in Ukraine and in host countries can use these helping strategies and resources to provide effective support for Ukrainians and for war refugees in general.
Job insecurity during the COVID-19 pandemic and counterproductive work behavior: The sequential mediation effects of job stress and organizational identification and the buffering role of corporate social responsibility
Authors: Kim Byung-Jik , Lee Julak , Jung Jeyong , Kim Min-Jik
Published: February 2023

Swift social and economic environmental changes such as those associated with the COVID-19 pandemic have led to decreased job security. Although numerous previous studies have examined the influence of job insecurity on employee perceptions, attitudes, and behaviors, the link between job insecurity and negative behavior and its underlying or intermediating mechanisms remain underexplored. The significance of an organization's positive behaviors, which fall under the umbrella of corporate social responsibility (CSR), also deserves more attention. To address these gaps, we examined both the mediator and the moderator in the association between job insecurity and negative employee behavior by establishing a moderated sequential mediation model. We hypothesized that the levels of employee job stress and organizational identification sequentially mediate the relationship between job insecurity and counterproductive work behavior as a representative negative behavior. We also hypothesized that CSR activities play a buffering role that moderates the influence of job insecurity on job stress. We used three-wave time-lagged data collected from 348 employees in South Korean organizations to demonstrate that job stress and organizational identification sequentially mediate the relationship between job insecurity and counterproductive work behavior, and that CSR activities function as a buffering factor that decreases the influence of job insecurity on job stress. The results of this research suggest that the levels of job stress and organizational identification (as sequential mediators) as well as CSR activities (as a moderator) are underlying mechanisms in the link between job insecurity and counterproductive work behavior.
“Parenting my parents”: Perspectives of adult children on assuming and remaining in the caregiver's role
Authors: Charenkova Jūratė
Published: February 2023

Background

Family caregivers are essential when responding to the long-term care needs of aging societies. The complex and multifaceted caregiver's role encompasses a unique set of challenges and strains, however, it can be a rewarding experience with many benefits and positive outcomes. Moreover, there is a link between the caregiver's wellbeing, quality of care, and the quality of life of the care recipient. Thus, the current study aimed to explore why adult children are assuming and remaining in the caregiver's role despite its challenges.

Methods

Research data was collected through the use of qualitative semi-structured interviews from September 2021 to July 2022. In total 16 Lithuanian and Italian caregivers were recruited through convenience/snowball sampling. The study utilized the constructivist grounded theory for data analysis and self-determination theory for data interpretation.

Results

Adult children's caregiving experiences revealed three themes related to the motivation to assume and continue with family care: (1) believing in the inherent value of family care; (2) making sense of the changing nature of caregiving; and (3) “making the best of it”. Key motivational drivers of these decisions were associated with the satisfaction of the three basic psychological needs – autonomy, competence, and relatedness. Results show that finding meaning and making sense of the caregiving role when responding to a parent's increased care needs may result in positive caregiving experiences and outcomes even at rather low levels of the care recipient's autonomy.

Conclusion

Caregivers were able to experience family care as a meaningful and rewarding experience while acknowledging its challenges and limitations. Implications for family caregiving decisions and experiences, social policy, and future research are discussed in more depth in the paper.