bluuebunny/crossref_metadata_2025 huggingface dataset:
CONCEPÇÕES DE SAÚDE E DE ATUAÇÃO PROFISSIONAL DOS RESIDENTES DE EDUCAÇÃO FÍSICA DE UMA RESIDÊNCIA MULTIPROFISSIONAL EM SAÚDE
Authors: MANSKE GEORGE SALIBA , Linhares Ana Carolina Cruz , Silva Vitória Leal , Máximo Carlos Eduardo
Published: February 2022

O propósito deste trabalho foi compreender a influência da Residência Multiprofissional em Saúde (RMS) de uma Instituição de Ensino Superior (IES) do sul do Brasil na formação da concepção de saúde e de atuação dos profissionais de Educação Física na saúde pública, pelos residentes em formação. Foi realizada uma pesquisa de abordagem qualitativa, tendo como técnica de coleta de dados entrevistas semiestruturadas, seguida de análise temática. Percebeu-se uma diferença nas concepções de saúde em período anterior ao da RMS e posterior ao ingresso a ela, assim como, nas interpretações do fazer profissional em saúde pública pelos residentes em formação.
REFLEXIONES EN TORNO A LA EDUCACIÓN DE LOS CUERPOS EN LA CIUDAD EN EL MARCO DE LA EXTENSIÓN UNIVERSITARIA: EL CASO DEL CENTRO CULTURAL EN EL BARRIO FLOR DE MAROÑAS DE MONTEVIDEO
Authors: Pérez Monkas Gonzalo , Ríos Camilo , Rivas Camilo
Published: February 2022

El presente artículo recoge las reflexiones de un equipo universitario denominado Espacio de Formación Integral: Interdisciplina, Territorio y Acción Colectiva, de la Universidad de la República. En este sentido a raíz de la experiencia de extensión en un barrio popular de Montevideo se abordan algunas líneas en torno a ciertos discursos que circulan a propósito de la creación de un Centro Cultural en un espacio público. El proceso de discusión y diálogo entre actores gubernamentales, organizaciones barriales abre algunas discusiones que dejan entrever la configuración de una educación de los cuerpos y las distintas tensiones que se suscitan en la experiencia urbana.
A Mesoarchean Large Igneous Province on the Eastern Kaapvaal Craton (Southern Africa) Confirmed by Metavolcanic Rocks from Kubuta, Eswatini
Authors: Schneider K. P. , Hoffmann J. E. , Kröner A. , Balduin A. , Sprung P. , Schleicher A. M. , Münker C.
Published: February 2022

Mesoarchean magmatism is widespread on the eastern margin of the Kaapvaal Craton, but its origin is still poorly understood, mainly because geochemical data is rare. To shed some light on the source of this Mesoarchean magmatism and to relate different Mesoarchean volcanic sequences to each other, we provide major and trace element data as well as Hf-Nd isotope compositions of amphibolites sampled close to the Kubuta Ranch in south-central Eswatini. These amphibolites, so far, were of unknown correlation to any volcanic sequence in Eswatini or South Africa. Hence, the aim of our study is to characterize the mantle source composition of these volcanic rocks and, furthermore, to constrain their genetic relation to other volcanic sequences in Eswatini and South Africa. Our findings reveal that, based on coherent trace element patterns and similar Nd isotope characteristics, the Kubuta volcanic rocks can be genetically linked to the ca. 3.0 Ga Usushwana Igneous Complex in West-Central Eswatini and the ca. 2.9 Ga Hlagothi Complex located in the KwaZulu-Natal province. In contrast, the coeval ca. 3.0 Ga Nsuze and ca. 2.9 Ga Mozaan Groups (Pongola Supergroup) of south-central Eswatini and northern KwaZulu-Natal province have slightly enriched compositions compared to the newly sampled Kubuta volcanic rocks. Our results suggest that the Nsuze and Mozaan Groups were sourced from a primitive mantle reservoir, whereas the Usushwana, Hlagothi, and Kubuta mafic rocks were derived by melting of a more depleted mantle source comparable to that of modern depleted MORB. Furthermore, our assimilation-fractional crystallization (AFC) calculations and Nd isotope constraints reveal that some samples were contaminated by the crust and that the crustal contaminants possibly represent felsic rocks related to the ca. 3.5 Ga crust-forming event in the Ancient Gneiss Complex. Alternatively, melting of a metasomatized mantle or plume-lithospheric mantle interaction may also produce the trace element and isotopic compositions observed in the samples. From a synthesis of our geochemical observations and age data from the literature, we propose a refined petrogenetic model, for a continental flood basalt setting in a Mesoarchean large igneous province on the eastern Kaapvaal Craton. Our petrogenetic model envisages two magma pulses sourced from a primitive mantle reservoir that led to the formation of the Nsuze (first) and Mozaan (second) lavas. Conductive heating of ambient depleted mantle by the mantle plumes caused partial melting that led to the formation of the Usushwana Igneous Complex associated with the first magmatic event (Nsuze) and the Hlagothi Igneous Complex associated with the second magmatic event (Mozaan). However, due to lacking age data of sufficient resolution, it is not possible to affiliate the Kubuta lavas to either the first or the second magmatic event.
CD40 Monoclonal Antibody and OK432 Synergistically Promote the Activation of Dendritic Cells in Immunotherapy
Authors: Zhang Juan , Wang Lei , Li Shuyi , Gao Xuefeng , Liu Zhong
Published: February 2022

Background: Colorectal cancer (CRC) with pulmonary-metastasis usually indicates a poor prognosis, whereas patients may benefit from adoptive cell therapy. Tumor specific cytotoxic T lymphocytes (CTLs) have been reported to be a promising treatment for CRC. However, the antitumor effect of CTLs remains limited, partially due to insufficient production of effector cells via the activation with antigen-presenting dendritic cells (DCs). Method: Here we presented that a combination of CD40 mAb and Picibanil (OK-432) could significantly enhance the activation of CTLs by DCs, both in vitro and in vivo. Flow cytometric, colon cancer mouse model, and pathological staining were employed to demonstrate the specific functions. Results: This approach promoted the maturation of DCs, augmented the production of stimulatory cytokines, and suppressed the secretion of inhibitory cytokines. Additionally, it facilitated the killing efficiency of CTLs via stimulating their proliferation while restrained the number of Tregs, concomitantly with the positive regulation of corresponding cytokines. Furthermore, the combined unit could hurdle the expansion of tumor cells on metastatic lung in colon cancer mouse model. Conclusion: Collectively, the combination of CD40-mAb and OK-432 can facilitate the maturation of DCs and enhance the cytotoxicity of T cells, which may be a promising approach for clinical treatment against CRC.
Evaluation of the anticonvulsant activities of Gastrodia elata Bl.-Acorus tatarinowii decoction on experimentally induced seizures in mice
Authors: He Xirui , Yang Yan , Yuan Xufang , Sun Yin , LI Yongsheng
Published: February 2022

Epilepsy is a serious public health problem in the world. At present, over 30% of affected patients remain refractory to current available treatment. Medicinal plants as pharmaceuticals and healthcare treatments have been frequently used in the management of epilepsy in China for many centuries. Gastrodia elata Bl.-Acorus tatarinowii (GEAT), as a classic and most commonly used herb pair in in traditional Chinese medicine (TCM), has been employed to control seizures for thousands of years. However, the animal experiment data of its anticonvulsant effect is limited in the literature. The objective of this study was to mainly analyze the anticonvulsant activity of GEAT decoction in maximal electroshock (MES), pentylenetetrazole (PTZ) and trimercaptopropionic acid (3-MP) induced acute seizures in mice, providing a scientific basis for the treatment of convulsive disorders in traditional medicine. In addition, PTZ-induced kindling models were conducted to investigate the seizure severity, anxiety and cognitive profile, inflammation, and oxidative stress parameters in mice. The results showed that GEAT decoction dose-dependently protected mice against MES, 3-MP and PTZ induced acute seizures. Meanwhile, GEAT decoction ameliorated seizure severity, decreased the accumulation of inflammatory mediators TNF-α, IL-1β, and IL-6, mitigated oxidative stress, as well as alleviated anxious-like behavior and cognitive deficits in PTZ-kindled mice. Our data evidenced that GEAT decoction possesses certain anticonvulsant properties, which might be clinically useful as a phytotherapy alone or as an adjunct therapy for the prevention and treatment of seizures and epilepsy.
Prevalence and Antimicrobial Susceptibility Pattern of Neisseria Gonorrhoeae from Selected Public Health Centers, Addis Ababa, Ethiopia.
Authors: Ayalew Enaniye , Wolde Mistire , Ebrahim Semira , Seyoum Elias , Woldesenbet Zerihun , Dinku Surafel Fentaw
Published: February 2022

Background: Neisseria gonorrhea (NG) is gram negative diplococcic bacteria that cause most important sexually transmitted infections (STIs) worldwide. In most developing countries including Ethiopia, NG infections are diagnosed syndromically, and its antibiotic susceptibility is rarely tested. Thus, the objective of the present study was to determine the prevalence and antimicrobial susceptibility patterns of N. gonorrhoeae among syndrome diagnosed and treated cases attending selected public health centers, in Addis Ababa, Ethiopia.Methods: a cross-sectional study was conducted from April 2019 to March 2020, at selected health centers STIs clinics in Addis Ababa, Ethiopia. Urethral discharge or vaginal discharge specimens were cultured on Modified Thayer Martín media and suspected gonococcal colonies were confirmed using Oxidase, Superoxol tests. Antimicrobial susceptibility testing was performed by Minimum Inhibitory Concentration (MIC) using concentration gradient strips (E-test) of the same antimicrobial agents of ciprofloxacin, penicillin, Ceftriaxone, Azytromicin, Cefoxitin, and SpectinomicinResults: Among the total 325 NG suspects 163 (50 %) were culture positive. Among the culture positive cases 126 were male and 37 females. Around 4% of isolates are non-susceptible to Ceftriaxone which is the current antimicrobial agent used for urethral discharge/ vaginal discharge syndrome treatment.Conclusion: In the current study, the proportion of N. gonorrhoeae culture positivity rate among male was found to be high. The antimicrobial agent ceftriaxone used for the treatment of gonococccal isolates found to become non susceptible. Antimicrobial agents outside of ciprofloxacin and natural penicillin antimicrobial agents are eligible for the national STI syndromic management.
Source tracking microplastics pollution from Qarasu basin rivers to Gorgan Bay, Caspian Sea
Authors: masoudi ebrahim , Hedayati Aliakbar , bagheri tahere , salati AmirParviz , safari Roghayeh , Gholizadeh mohammad , zakeri mohammad
Published: February 2022

Concerns about the negative effects of MPs on human health have led to increasing attention to the occurrence of MPs in the aquatic environment. Recent studies have focus from the marine environment to inland waters, particularly the spatio-temporal distribution of MPs in rivers for residential and agricultural area. Qarasu river basin, watershed to Gorgan Bay, is the site of many permanent rivers and the levels of MP pollution in those rivers are unknown. This research was conducted in three different type of land use: forest, residential and agricultural along 8 different rivers. A total of 9 fish species were identified and the presence of MPs in 87% of fish was confirmed. The prevalence of MP among species was Liza sp.> C.gibelio> G.holbrooki> R.rutilus> N.melanostomus> C.carpio = V.vimba> R.frisii> Barbus sp. The highest frequency of MPs was observed in Qarasu river (1880 ± 251 n/kg) and Mohammadabad river (184 ± 1340 n/kg) in agricultural land use, the lowest frequency was related to Baghu and Ziarat rivers with 660 ± 77 and 600 ±91 n / kg was found in forest land use respectively. The highest type, color and size of MP sediments were fragment, black and 1-2 mm, respectively. The highest type, color and size of MP in fishes were fiber, black and 0.5-0.1mm respectively. Overally according to the source tracking result, agricultural land use was the main source of MP pollution of Gorgan Bay. The Bay is strongly affected by the incoming rivers, the Qarasu River Basin as the most important river supplying water.
Remote sensing of boat abandonment using Google EarthTM
Authors: Payne Millicent R. , Turner Andrew
Published: February 2022

Abandoned boats represent a pervasive and growing problem in the coastal zone that have received little regulatory or scientific attention. In this study, we show how Google EarthTM can be employed to remotely identify abandoned boats and provide information on their size, age and condition. Based on specified criteria for abandonment, a survey of southern England (1700 km in length) reveals the presence of 266 boats that are mainly located in the intertidal zones of estuaries and inlets and that are often contained within clusters. Boat length ranged from 2.6 to 60 m (median = 10.5 m) and since 2004 abandonment has been increasing at a rate of about eight per year. The majority of boats appear to be of timber construction and exhibit evidence of damage and water-sediment ingression. The environmental impacts are likely to be most significant where boats are clustered in protected mudflats.
Predictors of Long-term Mortality After Intertrochanteric Fractures Surgery: A 3-year Retrospective Study
Authors: Lu Yao , Huang Qiang , Xu Yibo , Ren Cheng , Sun Liang , Dong Wenchao , Li Ming , Xue Hanzhong , Li Zhong , Zhang Kun , Ma Teng , Wang Qian
Published: February 2022

Introduction: Intertrochanteric fractures are associated with high mortality rates; however, long-term data on survival and predictors remain scarce. Therefore, this study investigated risk factors associated with 3-year mortality in elderly patients with intertrochanteric fractures. Methods: In a retrospective study, 156 elderly patients with intertrochanteric fractures who underwent surgery between January 2017 to January 2018 at our center were included. Association-affecting variables, such as gender, age, time from injury to surgery, hemoglobin (Hb), total lymphocyte count (TLC), albumin, malnutrition, and co-morbidities, were recorded and analyzed. Afterward, logistic regression was used to analyze the significant variables and find independent predictors for 3-year mortality. Results: A total of 156 patients were followed up for 3 years. The 1-year, 2-year, and 3-year postoperative cumulative mortality rates were 9.6% (15/156), 16.7% (26/156), and 24.4% (38/156), respectively. Simple analyses found that age, Hb, albumin, and malnutrition were associated with 3-year mortality (p<0.05). Multivariable analysis confirmed that advanced age (p<0.001) and low albumin (p=0.014) were independent risk factors for 3-year mortality. Conclusion: Low serum albumin and advanced age were independent risk factors for long-term mortality in elderly patients with intertrochanteric fractures.
Economic And Social Deprivation Predicts Impulsive Choice In Young Children.
Authors: Tunney Richard
Published: February 2022

Impulsivity is an individual difference in decision-making that is a risk factor for a number of health concerns including addiction and obesity. Although impulsivity has a large heritable component it, the health concerns associated with impulsivity are not uniformly distributed across society. For example, people from poorer backgrounds are more likely to be overweight, and be dependent on tobacco or alcohol. This suggests that the environmental component of impulsivity night be related to economic circumstances and availability of resources. This paper provides evidence that children aged 4 to 12 from the most deprived areas show greater impulsivity in the form of delay discounting than do children from the least deprived areas. The data are discussed with reference to scarcity based models of decision-making and to public health inequalities.
Transmission of SARS-CoV-2 from humans to animals and potential host adaptation
Authors: Tan Cedric , Lam Su Datt , Richard Damien , Owen Chris , Berchtold Dorothea , Orengo Christine , Nair Meera , Kuchipudi Suresh , Kapur Vivek , Dorp Lucy van , Balloux Francois
Published: February 2022

SARS-CoV-2, the agent of the COVID-19 pandemic, can infect a wide range of mammals. Since its spread in humans, secondary host jumps of SARS-CoV-2 from humans to a variety of domestic and wild populations of mammals have been documented. The evolution of SARS-CoV-2 in different host species is of fundamental interest while also providing indication of how SARS-CoV-2 may have adapted to human hosts soon after the initial host jump, a time window for which there are no genome sequences available. Moreover, the study of SARS-CoV-2 circulating in animals is critical to assess the risk that the transmission of animal-adapted viral lineages back into humans (i.e., spillback) may pose. Here, we compared the genomic landscapes of SARS-CoV-2 isolated from animal species relative to that in humans, profiling the mutational biases indicative of potentially different selective pressures in animals. We focused on viral genomes collected in infected mink (Neovison vison) and white-tailed deer (Odocoileus virginianus) for which reports of multiple independent spillover events and subsequent animal-to-animal transmission are available. We identified six candidate mutations for animal-specific adaptation in mink (NSP9_G37E, Spike_F486L, Spike_N501T, Spike_Y453F, ORF3a_T229I, ORF3a_L219V), and one in deer (NSP3a_L1035F), though these mutations appear to confer minimal advantage for circulation in humans. Additionally, circulation of SARS-CoV-2 in mink and deer has not caused considerable changes to the evolutionary trajectory of SARS-CoV-2 thus far. Finally, our results suggest that minimal adaptation was required for human-to-animal spillover and subsequent onward transmission in mink and deer, highlighting the ‘generalist’ nature of SARS-CoV-2 as a pathogen of mammalian hosts.
Protocatechuic Acid, Ferulic Acid and Relevant Defense Enzymes Correlate Closely with Walnut Resistance to Xanthomonas arboricola pv. Juglandis
Authors: Zhang Qian , Li Meixuan , Yang Guiyan , Liu Xiaoqiang , Yu Zhongdong , Peng Shaobing
Published: February 2022

Background: Juglans regia L. is an important nut tree that has a wide range of distribution in temperate regions of the world. In some walnut orchards, walnut blight can become a problematic disease that affects the growth of walnut trees. To explore the correlation between biochemical response and walnuts resistance, we inoculated four walnut cultivars with Xanthomonas arboricola pv. Juglandis (abv. Xaj). The walnut cultivars were, namely, ‘Xiangling’, ‘Xiluo 2’, ‘Yuanfeng’ and ‘Xifu 2’. Total phenol content (TPC) and total flavonoid content (TFC) were measured, whereby nine major phenolic compounds and several relevant enzymes were identified. Results: The results showed that the most resistant and susceptible walnut varieties were ‘Xiluo 2’ and ‘Xifu 2’ respectively. The reaction of walnut to Xanthomonas arboricola pv. Juglandis was characterized by the early accumulation of phenolic compounds in the infected site. The accumulation of phenolic compounds after infection was accompanied by an increase in reactive oxygen species (ROS). This was reflected in the catalytic effect of peroxidase (POD) on ferulic acid dimerization which contributed to walnut resistance against disease. This also meant a series of defense-related functions with a key role of polyphenol oxidase (PPO). The results revealed that the competitiveness of flavonoids were weakened in biosynthesis, thereby strengthening the synthesis of chemicals catalyzed by phenylalanine ammonia lyase (PAL) against walnut blight. The resistant variety ‘Xiluo 2’ had a significantly higher protocatechuic acid content, compared to other varieties, when measured on the 4th day after inoculation (dpi). Conclusion: Our findings confirmed that walnut blight resistance is a collective function of interactions between the accumulation of phenolic compounds in the phenylpropane metabolic pathway and the action of relevant defense enzymes.
Dynamic NLR and PLR in predicting Covid-19 severity: a retrospective cohort study
Authors: Asperges Erika , Albi Giuseppe , Zuccaro Valentina , Sambo Margherita , Pieri Teresa Chiara , Calia Matteo , Colaneri Marta , Maiocchi Laura , Melazzini Federica , Lasagna Angioletta , Peri Andrea , Mojoli Francesco , Sacchi Paolo , Bruno Raffaele
Published: February 2022

Background: the hyperinflammation phase of severe SARS-CoV-2 is characterized by complete blood count alterations. In this context, the neutrophil to lymphocyte ratio (NLR) and the platelet to lymphocyte ratio (PLR) can be used as prognostic factors. We study NLR and PLR trends at different timepoints and compute optimal cutoffs to predict four outcomes: use of Continuous Positive Airways Pressure (CPAP), ICU admission, invasive ventilation and death. Methods: we retrospectively included all adult patients with SARS-CoV-2 pneumonia admitted from 23rd January 2020 to 18th May 2021. Data were extracted using ICD9 codes and our Covid-19 registry. Analyses included descriptive statistics and non parametric tests to study the ability of NLR and PLR to distinguish the patients’ outcomes at each timepoint. ROC curves were built for NLR and PLR at each timepoint (minus discharge) in order to identify cutoffs to distinguish severe and non severe disease and their statistical significance was assessed with the Chi-square test. NLR and PLR were compared with DeLong’s test. Results: we included 2169 patients. NLR and PLR were higher in severe Covid-19 at all the timepoints, with a difference that gets bigger and a trend that is steeper in more severe disease. Both ratios were able to distinguish the outcomes at each timepoint. For NLR, the areas under the curve (AUROC) ranged between 0.59 and 0.81, for PLR, between 0.53 and 0.67. From each ROC curve we computed an optimal cutoff value (e.g. NLR 7 for CPAP for males at admission). NLR performed better than PLR. Conclusion: our results are in line with other studies that computed NLR and PLR trends and values in disease, especially with those that distinguished between different grades of severity. Our study is retrospective and single centre, and is limited by selection bias, but includes more than 2000 patients, thus limiting the confounding factors and outliers. Our cutoffs do not only deal with severity and mortality but allow for a more tailored approach. Future prospect include validating our cutoffs in a prospective cohort and comparing their performance against other Covid19 scores.
Lycorine Inhibits Angiogenesis by Docking to PDGFRα
Authors: Lv Fei , Li XiaoQi , Hao LiYing , Wang Ying
Published: February 2022

Lyc (Lycorine) is a natural alkaloid derived from medicinal plants of the Amaryllidaceae family. Lyc has been reported to inhibit recurrence and metastasis of different kinds of tumors. However, the Lyc’s effect of angiogenesis and its specific mechanism is still not clear. This study was designed to test the anti-angiogenesis effect of Lyc and explore possible mechanisms. We performed cell experiments to confirm Lyc's inhibitory effect on angiogenesis and employed sunitinib as a positive control. Meanwhile, the synergistic effect of Lyc and sunitinib was also explored. Next, we conducted bioinformatics to predict the potential targets of Lyc and verified them by western blott and immunofluorescence. Molecular docking, kinase activity assays, Biacore assays and cellular thermal shift assays (CETSAs) were applied to elucidate the mechanism by which Lyc inhibited target activity. Lyc inhibited angiogenesis in human umbilical vein endothelial cells (HUVECs). Employing bioinformatics, we found that Lyc's target was PDGFRα and that Lyc attenuated PDGFRα phosphorylation. We also found that Lyc inhibited PDGFRα activation by docking to it to restrain its activity. In addition, Lyc significantly inhibited PDGF-AA-induced angiogenesis. This study provides new insights into the molecular functions of Lyc and indicates its potential as a therapeutic agent for tumor angiogenesis.
Modern contraception use and associated factors in Ethiopia: Evidence from the 2019 Ethiopian Mini Demographic and Health Survey
Authors: Zeleke Girum Taye , Zemedu Theodros Getachew
Published: February 2022

Background: The use of contraceptive is key in reducing unsafe abortion from unintended pregnancies, infant mortality, adolescent pregnancies, slowing population growth and helps to prevent HIV/AIDS. However, less than one-third of women within reproductive age in Ethiopia uses modern contraceptive methods. Hence, this study aims to identify potential factors on use of modern contraceptive method.Methods: Data from 2019 Ethiopian min Demographic and Health Survey were used in this analysis. A total of 8,885 women within the ages of 15–49 years across 305 enumeration areas in nine regions and two city administrations were included in the analysis. Logistic regression model used to examine the association between women’s background characteristics and modern contraceptives use. Results: Only 28.1% of women used modern contraceptives. About 40% of the modern contraceptive users were between age of 25-29 and 30–34 years. There was significant association between women’s age, level of education, region, religion, parity, wealth quintile and marital status on use of modern contraceptives. Women who were married and living with partners were about 20 and 24 times more likely to use modern contraceptives compared to sexually active unmarried women. Conclusion: The study showed that the use of modern contraceptive method is low and influenced by socio-demographic characteristics of women in Ethiopia. Therefore, increasing the awareness of women to use modern contraceptive methods is vital.
The diagnostic value and therapeutic predictive value of immune genes in psoriasis
Authors: Pan Jiax , Li Yuzhen
Published: February 2022

Background: We already know that abnormal immune cell infiltration is related to the pathogenesis of psoriasis (PSO), but the role of immune cell infiltration in the pathogenesis and treatment of psoriasis is unknown.The purpose of this study was to determine the diagnostic and predictive value of immune-related genes in psoriasis.Methods: Six gene expression datasets were downloaded from GEO database to analyze gene expression in psoriasis tissues. Single-sample gene set enrichment analysis (ssGSEA) was used to assess immune cell infiltration in psoriatic tissue. Immune-related genes were screened by overlapping immune-related genes with differentially expressed genes (DEGs). Protein interaction (PPI) networks are used to identify key DEGs. We analyzed the diagnostic value of key genes in psoriasis and the predictive value of key genes in three types of biological therapy. Immunohistochemistry (IHC) was used to detect gene expression in psoriasis tissues.Results: There were significant differences in immune cell infiltration between psoriatic tissue and normal skin tissue. PRC1, GATA3, IL1RN and CCL20 are the key genes of four subclusters in PPI network. PRC1, GATA3, IL1RN and CCL20 have diagnostic value in psoriasis, among which PRC1 and CCL20 have better diagnostic value than GATA3 and IL1RN. After treatment with effective biologic agents, the expression of PRC1, GATA3, IL1RN and CCL20 decreased in skin tissues of patients. GATA3 and IL1RN did not change significantly in patients with non-response to Ustekinumab, and the expression of GATA3 did not change significantly in patients with non-response to Etanercept, that showed good predictive value. Immunohistochemical analysis confirmed that the expression of PRC1, GATA3, IL1RN and CCL20 in PSO tissue was significantly different from that in normal skin tissue, and immune-related genes PRC1, GATA3, IL1RN and CCL20 could be used as auxiliary diagnostic indicators of psoriasis. GATA3 and IL1RN may serve as promising predictors of tumor necrosis factor inhibitors and IL-12/23 inhibitors therapy in patients.Conclusion: This study reveals the significance of immune genes in the pathogenesis and treatment of psoriasis. These key genes may provide new ideas for future treatments.
Comparing the Effects of Facility-Specific Guideline and Nudge-Based Antimicrobial Stewardship at Pediatric Primary Emergency Medical Centers
Authors: Otake Shogo , Kusama Yoshiki , Tsuzuki Shinya , Myojin Shota , Kimura Makoto , Kamiyoshi Naohiro , Takumi Toru , Ishida Akihito , Kasai Masashi
Published: February 2022

Purpose: Antimicrobial prescription rates tend to be high in outpatient settings. Primary emergency medical centers (PECs) in Japan encounter difficulties in implementing antimicrobial stewardship programs (ASPs). While a nudge-based ASP significantly reduces inappropriate prescription of oral third-generation cephalosporins (3GCs), publishing monthly newsletters requires considerable effort. Therefore, developing more preferable ASP models in PECs is essential. Methods: We conducted a multi-center, quasi-experimental study. The Himeji Primary Emergency Medical Center for Nights and Holidays (Site A) introduced the facility-specific guideline referring to national guidelines. The Kobe Children's Primary Emergency Medical Center (Site B) provided the results of the monitoring for antibiotics prescription as a monthly newsletter. The Hanshin-Kita Children’s First-Aid Center (Site C) did not perform the specific ASP. Prescription rates for 3GCs were categorized into pre- and post-intervention and compared using Poisson regression analysis. The difference-in-difference method was used to assess the effect of these interventions. Results: The numbers of patients pre- and post- intervention were 177,126 and 91,251, respectively. The 3GCs prescription rate at Site A, Site B, and Site C decreased from 6.7%, 4.2%, and 6.1% in 2016 to 2.3%, 1.0%, and 2.0% in 2019, respectively. Site B had a greater reduction than Site A and Site C (odds ratio [OR] 0.81 [95% confidence interval (CI) 0.70–0.93]; P<0.001, OR 0.76, [95%CI 0.66–0.87]; P=0.004). There was no significant difference between Site A and Site C (OR 0.94 [95%CI 0.83–1.06]; P=0.33). Conclusions: Facility-specific guideline was less effective than nudge-based ASP for decreasing oral third-generation cephalosporins prescription in PECs.
Expression of Annexin A5 in maternal blood and placenta in pregnancies with preeclampsia
Authors: Teng Meng , Yu Jing , Pan Yidan , Yang Qiyuan , Zhang Binbin , Wu Suihan , Weng Zhanping
Published: February 2022

Background

The placental anticoagulant protein Annexin A5 is highly expressed on the apical surfaces of syncytiotrophoblasts and plays an important role in maintaining blood fluidity in the placental circulation. We investigated the expression of Annexin A5 in maternal blood and placentas from pregnancies complicated by preeclampsia compared with uncomplicated pregnancies.

Materials and Methods

Placental tissue and maternal blood were collected from pregnancies complicated by preeclampsia. They were classified into two groups: early-onset group (n = 10), late-onset group (n = 10) .10 women without perinatal complications who accepted elective term cesarean section were chosen as the control group. Western blot and immunohistochemistry were used to detect the expression and localization of Annexin A5 in the placenta. Annexin A5 mRNA expression was quantified by Real-Time PCR. Annexin A5 and coagulation factor Xa in maternal blood was measured by an enzyme-linked immunosorbent assay. Prothrombin time, activated partial thromboplastin time, Thrombin time, fibrinogen were detected in each groups.

Results

(1) The expression of Annexin A5 in maternal blood were: 5.54±0.29 in early-onset group; 5.10±0.28 in late-onset group; 4.5±0.19 in control group. They were significantly higher in preeclampsia groups than in control group (P = 0.013). However, there was no significant difference among preeclampsia groups (P > 0.05). The mean ±SD coagulation factor Xa expression in the early-onset and late-onset group were 1229.23±45.33 and 1366.96±56.30 versus 1372.95±45.44 in the control group, which has not statistically significant(P=0.072). (2) The protein expression of Annexin A5 in placenta was not statistically significant between the early-onset, late-onset, and control groups (mean ± SD): 0.01 ± 0.02, 0.95±0.03vs.0.99 ± 0.02, p = 0.071.(3) The Annexin A5 mRNA levels in placenta were: 0.79±0.23 in early-onset group; 1.18±0.53 in late-onset group; respectively. All were significantly lower than that in control group (6.19±1.68, P =0.024), and no significant difference was found among preeclampsia groups (P > 0.05). (4) Prothrombin time, activated partial thromboplastin time, Thrombin time, fibrinogen levels were: (11.9 ± 0.23), (33.38± 0.85) s, (16.88 ± 0.45) g/L and 3.85 ± 0.23 in early-onset group; (11.65 ± 0.12), (33.15 ± 0.36) s, (16.13 ± 0.19) g/L and 4.91 ± 0.14 in late-onset group; (12.01 ± 0.09), (32.08 ± 0.41) s, (16.34 ± 0.24) g/L and 4.32 ± 0.20 in control group. The first three were no significant difference among preeclampsia groups and control group (P > 0.05), fibrinogen was statistically significant among all groups (P < 0.05).

Conclusion

These results suggest that Annexin A5 plays an important role in the pathogenesis and progression of preeclampsia by affecting coagulation.
Prenatal and postpartum maternal mental health and neonatal motor outcomes during the COVID-19 pandemic
Authors: Papadopoulos Alissa , Nichols Emily S. , Mohsenzadeh Yalda , Giroux Isabelle , Mottola Michelle F. , Lieshout Ryan J. , Duerden Emma G.
Published: February 2022

Background
Rates of prenatal and postpartum stress and depression in pregnant individuals have increased during the COVID-19 pandemic. Perinatal maternal mental health has been linked to worse motor development in offspring, with motor deficits appearing in infancy and early childhood. We aimed to evaluate the relationship between prenatal and postpartum stress and depression and motor outcome in infants born during the COVID-19 pandemic.
Methods
One hundred and seventeen participants completed an online prospective survey study at two timepoints: during pregnancy and within 2 months postpartum. Depression was self-reported using the Edinburgh Perinatal/Postpartum Depression Scale (EPDS), and stress via the Perceived Stress Scale (PSS). Mothers reported total infant motor ability (fine and gross) using the interRAI 0–3 Developmental Domains questionnaire.
Results
Prenatal (B=-0.035, 95%CI=-0.062 to -0.007, p = .014) and postpartum maternal depression outcome (B=-0.037, 95%CI= -0.066 to -0.008, p = 0.012) were significant negative predictors of total infant motor ability (fine and gross). Neither pregnancy nor postpartum perceived stress predicted infant motor function. A cluster analysis revealed that preterm and low birth weight infants belonging to mothers who reported elevated depressive symptoms during pregnancy and in the postpartum period had the poorest motor outcome.
Conclusions
Prenatal and postpartum depression, but not stress, predicted infant motor ability during the first 2 months of life. Preterm and low-birth weight infants of mothers who report elevated depressive symptoms are the most at-risk of experiencing poor motor outcome.
HIF-1α upregulation exerts the antagonistic effect against angiogenesis inhibition in manganese deficiency-induced tibial dyschondroplasia of broiler chicks
Authors: lu lu , Jin Cong , Dong Peng-Fei , Wang Lin , Wang Zhen-Yong
Published: February 2022

Manganese (Mn) is an essential microelement for broiler breeding and its deficiency causes tibial dyschondroplasia (TD). Tibial growth plate (TGP) development and metaphyseal vascularization are crucial for tibia growth in fast-growing broiler chickens, but their roles in Mn deficiency-induced TD in chicks remains unclear. This study was designed to clarify this issue. A total of 36 one-day-old broilers were divided into the control group and Mn-deficiency (Mn-D) group, which were fed with a standard diet (60 mg Mn/kg) and Mn deficiency diet (22 mg Mn/kg) for 42 days, respectively. TGP and proximal tibial metaphysis were collected to perform the related assays. First, Mn deficiency decreased the tibia length and TGP thickness in TD model. Mn deficiency reduced the number of blood vessels in proximal tibial metaphysis by histological analysis. Also, Mn deficiency decreased the mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and macrophage migration inhibitory factor (MIF) in TGP. Moreover, up-regulation of hypoxia-inducible factor-1 alpha (HIF-1α) at both mRNA and protein levels was found in TGP of Mn-D group, suggesting the antagonistic role of HIF-1α against angiogenesis inhibition. Additionally, Mn deficiency enhanced the autophagy related 5 (ATG5) and microtubule associated protein 1 light chain 3 beta (LC3-II) expression levels, but decreased the SQSTM1 (p62) expression levels in TGP, suggesting autophagy activation in this process. Collectively, these data indicate that HIF-1α up-regulation and concurrent autophagy activation exert the protective effect against Mn deficiency-induced angiogenesis inhibition, which may provide a useful guidance to prevent TD in broilers.
Sexually Transmitted Infections (STIs) increased the risk of precancerous cervical lesions among women in Ethiopia: unmatched case-control study
Authors: Teklehaimanot Dereje Abebe , Derseh Behailu Tariku , Mekuria Abinet Dagnew , Dadi Abel Fekadu
Published: February 2022

Objective: This study aimed to identify the determinants of cervical pre-cancerous lesions among women who attend cervical cancer screening in public health facilities.Design: An institution-based, unmatched case-control study was conducted among 216 women (54 cases and 162 controls) from January 2020 to April 2020. Settings: Selected public health facilities in North Shoa Zone, Amhara, Ethiopia.Participants: Women aged 30 - 49 who were attending cervical cancer screening at health institutions were included in this study. Outcome measures: Cases were women who attended cervical cancer screening and tested positive for Visual Inspection with Acetic Acid (VIA), whereas controls were women who tested negative for VIA. Results: Women who used oral contraceptives for more than 5 years were approximately 5 times more likely to develop pre-cancerous lesions (Adjusted Odds Ratio (AOR) = 4.75; 95% CI: 1.48, 15.30), and early age at first sexual intercourse (<15 years) increased the odds of developing pre-cancerous lesion by 4 fold (AOR = 3.77; 95% CI: 1.46, 9.69). Women having HIV seropositive results and had a previous history of sexually transmitted infections (STIs) had a 3.4 (AOR = 3.45; 95% CI: 1.29, 9.25) and 2.5(AOR = 2.58; 95% CI: 1.10, 6.09) times more odds of developing cervical pre-cancerous lesions than their counterparts. Conclusions: the use of oral contraceptives for more than five years, early initiation of sex, past and current STI increased the odds of women’s developing pre-cancerous cervical lesions. Awareness creation and the National cervical cancer screening program should prioritize women with identified exposures.
Implementation of Back At work After Surgery (BAAS): a feasibility study of an intergrated pathway for improved return to work after knee arthroplasty
Authors: Strijbos Daniel Otto , Sluis Geert van der , Boymans Tim A.E.J. , de Groot Stephan , Klomp Simon , Kooijman Carolien M. , Reneman Michiel F. , Kuijer Paul P.F.M.
Published: February 2022

Purpose
Optimizing return to work after knee arthroplasty becomes more important because of the growing incidence of KA among workers and poor return to work outcomes. The purpose of this study is to investigate the feasibility of Back At work After Surgery (BAAS): an integrated clinical pathway for return to work after knee arthroplasty.
Method
Working patients who received unicompartmental knee arthroplasty (UKA) or total knee arthroplasty (TKA) between January 2021 and November 2021, younger than 65 years and motivated to return to work were eligible to participate. Feasibility was investigated on five domains: reach, dose delivered, dose received, fidelity and patients’ attitudes. These outcomes were obtained by a patient-reported questionnaire and an interview with the case manager occupation and case manager medical.
Results
Of the eligible 29 patients, eleven were willing to participate (response rate 29%; due to travel distance to and from the hospital). The reach from professionals was 100%. The dose delivered between 91–100%, except information given about return to work from the orthopedic surgeon which was 18%. The dose received was 100%. For fidelity, case managersreported nine shortcomings for which five solutions were mentioned. In terms of patients’ attitude, all patients were satisfied and one patient mentioned an improvement.
Conclusions
In terms of reach, participation was low: only 29%. In terms of dose delivered, dose received, fidelity and patient attitudes of the BAAS clinical pathway seems feasible. The next step will be to assess the effectiveness of the BAAS clinical pathway for return to work.
Spatial targeting and prediction of factors responsible for optimum Covid-19 based social distancing – the case of Upper west region, Ghana
Authors: Asori Moses , Musah Ali , Peprah Prince
Published: February 2022

Covid-19 has become a significant global public health concern due to its faster rate of mortality and morbidities. Various precautionary measures such as social distancing have been outlined across the globe to reduce its transmission. However, the practicality of implementing the social distancing is a function of multiple factors including; urban morphology, population density and availability of essential socioeconomic services. Using Getis-Ord G* and Spatial-Cluster outlier analysis, we targeted the hotspot centres where effective social distancing will be difficult to achieve within Upper West Region in order to optimize intervention and resource allocation. We also examined factors determining effective Social Distancing using Geographically Weighted Regression (GWR). Ease of achieving social distancing index (ESDI) was acquired from WorldPop Group Research centre at Southampton University, Freshwater availability from Socioeconomic Data and Applications Center (SEDAC) and Average household size from DHIMS-2. Our spatial statistical analysis indicates that ESDI is significantly spatially auto-correlated; with hotspots occurring generally at the urban core. Using both the global (OLS) and local (GWR) regression models, we found that, relationships between ESDI and built-up area, built-up proportion, population density and freshwater availability were statistically significant. We suggest that areas where it is difficult to achieve social distancing due to built-up design or population density, constant surveillance should be adopted for timely detection.
REQUISITOS E INDICADORES DE AVALIAÇÃO PARA CENTRAIS DE ABASTECIMENTO FARMACÊUTICO
Authors: Batista Padilha Santos Mayara , Rodrigues de Castilho Selma , Teixeira Pontes André
Published: February 2022

Objetivo: Identificar os requisitos e indicadores de avaliação das atividades desenvolvidas em Centrais de Abastecimento Farmacêutico (CAF) de gestão pública.

Método: Trata-se de uma scoping review realizada a partir de consulta aos bancos de dados Scopus, PubMed, Google Acadêmico, SCIELO, websites da ANVISA e do CFF.

Fundamentação teórica: As CAF centralizadas são responsáveis pelo armazenamento e distribuição de medicamentos diretamente às unidades de dispensação dos municípios. A avaliação das CAF constitui uma ferramenta de apoio à gestão no aprimoramento dos processos e redução de custos.

Resultados: Foram selecionados 57 documentos da literatura científica e cinzenta. A maior parte dos estudos demonstrou problemas com o armazenamento e distribuição de medicamentos, assim como gerenciamento de recursos humanos nas CAF. A legislação mais recente prevê mudanças no setor de logística farmacêutica. Foi elaborado um modelo com requisitos e indicadores para CAF, que considera legislação atualizada.

Conclusões: Os resultados apresentados fomentam a criação de um instrumento de avaliação para detectar oportunidades de melhoria nos estabelecimentos e direcionar a tomada de decisão.
INFLUÊNCIA DA GESTÃO DA CADEIA DE SUPRIMENTOS HOSPITALAR NOS CUSTOS E AGREGAÇÃO DE VALOR
Authors: Diniz Octaviano Luana , Machado de Souza Lima Fátima
Published: February 2022

Objetivo: analisar as metodologias da GCS hospitalar, a fim de descrever como elas influenciam nos custos e agregação de valor. Método: trata-se de uma revisão integrativa para analisar a literatura existente sobre o tema, a partir de estratégia de busca definida em bases de dados específicas. A amostra foi composta por 20 estudos primários de um universo de 548 artigos. Fundamentação teórica: Nos últimos anos, os custos com a atenção hospitalar no Brasil têm aumentado, e os serviços de saúde procuram cada vez mais estabelecer vantagem competitiva através de processos que reduzam custos e gerem valor ao paciente. Resultados: evidenciou-se fragilidades no gerenciamento da cadeia de suprimentos hospitalar, como a falta de integração de informações e processos e o alto custo logístico demandado. Apesar de ainda pouco explorados, há utilização crescente de métodos de gestão por parte das instituições prestadoras do cuidado em saúde, a fim de atingir a eficiência da cadeia de suprimentos. Conclusões: indica-se a necessidade de aprimorar a GCS hospitalar e desenvolver os colaboradores que participam de seus processos, para que seja alcançada a redução dos custos e a agregação de valor.
CLINICAL-ACADEMIC NURSING CAREERS AND INSTITUTIONAL THEORY
Authors: Almeida Migowski Sérgio , Migowski Eliana
Published: February 2022

This study aims to propose a theoretical framework based on Institutional Theory in order to implement clinical-academic nursing careers (CAC), since nurses who combine care work with research tend to share best clinical practices with their colleagues, nursing students and even with their teachers.Method: The literature on CAC careers, evidence-based practice (EBP), health errors, continuing education, communities of practice and Institutional Theory was examined.Theoretical foundation: As theoretical support, we adopted literature about CAC, evidence-based practice, errors in healthcare, continuing education, communities of practice and Institutional Theory. A theoretical framework was created with five steps. Results: By introducing the CAC, the use of evidence-based practice will generate cost reduction, increased quality of services provided and greater satisfaction for these professionals.Conclusions: This study has international relevance, as it can be applied in any country that wants quality gains in the assistance provided by hospitals as well cost reduction.
TELEGESTÃO: PROPOSTA PARA CAPACITAR OS GESTORES DA ATENÇÃO PRIMÁRIA À SAÚDE EM MINAS GERAIS.
Authors: Costa Luciana , Godoy Solange , De Almeida Vanessa , Campos Kátia
Published: February 2022

O presente estudo teve como objetivo apresentar a criação do Projeto Telegestão a partir das experiências exitosas do Telenfermagem, com a finalidade de capacitar as Unidades de Serviços de Saúde dos municípios de Minas Gerais cadastrados pelo Programa Nacional de Telessaúde Brasil Redes. Trata-se de um relato da criação do Projeto Telegestão para contribuir no processo de educação no trabalho, permitindo que os profissionais da saúde, realizem a sua capacitação profissional no próprio local de trabalho. Será utilizado como mecanismos de trabalho a interligação da rede informatizada do Núcleo de Telessaúde da UFMG e o apoio do Laboratório de Computação Científica. O projeto contribui para a compreensão da necessidade de capacitação dos profissionais e aprimorar o cuidar e a gestão na APS dentro da organização do trabalho. Estima-se que a participação do público em cada webconferência atinja de 20 a 50 profissionais gerentes de unidades de saúde, considerando o total de municípios de Minas Gerais cadastrados pelo Programa Nacional de Telessaúde Brasil Redes. Espera-se contribuir para aprimorar o cuidar e a gestão dentro da organização do trabalho.