bluuebunny/crossref_metadata_2025 huggingface dataset:
Pediatricians’ Attitudes and Practices on ADHD Before and After the Development of ADHD Pediatric Practice Guidelines
Authors: Wolraich Mark L. , Bard David E. , Stein Martin T. , Rushton Jerry L. , O'Connor Karen G.
Published: August 2009

Purpose: The study aims to assess the changes in attitudes and practices about ADHD reported by AAP fellows between 1999 and 2005 during which AAP ADHD guidelines, training, and quality improvement initiatives occurred. Method: The study assesses AAP-initiated surveys that were conducted between 1999 and 2005 and involving a random sample of 1,000 and 1,603 pediatricians, respectively. Results: The findings reveal that significant, although modest, increases occurred in pediatric practitioners’ self-reported adherence to the guidelines. About 81% of respondents reported routine use of formal diagnostic criteria (up from 67%), and 67% of the respondents routinely use ADHD teacher rating scales (compared to 49% in the 1999 survey). Findings further reveal that treatment with stimulant medications was used extensively by pediatricians from both surveys; more pediatricians in the 2005 survey reported use of a second stimulant if the first did not work, and still more reported almost always providing parent training, although the estimated number remained only about a quarter of the total; and greater familiarity with the initiatives predicted better reported adherence to the guidelines. Conclusion: The reported behaviors of practitioners have moved in the direction of greater adherence with the recommended AAP ADHD guidelines, and there was a positive response to, and a greater use of, the materials developed to enhance practice. The authors infer that practice changes may be due to many factors, including AAP guidelines and associated implementation efforts. Changing physician practices needs to be sustained through a continuing process that requires multiple, varying, sustained efforts directed at physicians, other providers, and families.
‘‘Japanese Eyes, American Heart’’
Authors: Koikari Mire
Published: August 2009

Japanese American veterans’ narratives of World War II (WWII) constitute a dynamic discursive site where heterogeneous and contradictory understandings of masculinity, military service, and national allegiance are articulated. While loyalty and patriotism often stand out as dominant themes in their narratives, their retelling of wartime military service by no means provides a uniform picture of heroic masculinity and patriotic nationalism. Having suffered from race- and gender-based dislocation as colored immigrant men in US history, Japanese American veterans’ narratives necessarily include moments of ambiguity, contradiction, and disruption and reveal the complex nature of minority men’s assertion to national belonging. By examining diverse and often contestatory voices of Japanese American veterans, this essay illuminates how minority men’s retelling of military service contradicts and even subverts the dominant understandings of war, masculinity, and nation.
Instrumental or Expressive?
Authors: Smiler Andrew P. , Kubotera Natsuki
Published: August 2009

Contextualized studies of gender have been published, but these studies typically compare men and women in a single context. In this study, we examined men’s expectations for women across two interpersonal contexts, dating and the workplace, with particular attention to the gender-related traits of expressiveness and instrumentality. Data were provided by eighty-seven unmarried, heterosexual young adult males. Significant differences were found for trait preference as a function of context, with young men reporting stronger preference for expressive traits in the romantic context and stronger preference for instrumental traits in the work context. Contextual differences became nonsignificant when egalitarian beliefs were added to the model. Discussion focuses on the importance of context for understanding gender.
A Few Good Boys
Authors: Johnson Brooke
Published: August 2009

Through four years of ethnographic participant observation, and in-depth interviews, this article examines how militarism and masculinity are bound together in the social space of a military-style charter school in Southern California. Drawing on the concept of hegemonic masculinity by Connell, and the discussion by Higate and Hopton on the reciprocal relationship between militarism and masculinity, this article examines the construction of a military hegemonic masculinity at the school. It also examines the nuances and effects of this particular form of hegemonic masculinity for both boys and girls and argues it is exemplified at the school through the acceptance and condonement of violence and the warrior hero archetype. While not all cadets at the school have access to, or can capitalize upon the advantages of this particular hegemonic masculinity, specifically black boys and girls, it is a powerful force that shapes social interactions, social patterns, and social identities for boys and girls.
Campaigning and the Catch-All Party
Authors: Smith Jennifer K.
Published: August 2009

Otto Kirchheimer’s well-known diagnosis of ‘catch-all’ partism in Western Europe rests on an implicit causal argument about the consequences of social change for political parties. This article takes up the causal story underlying Kirchheimer’s account and traces its implications for a specific, though central, party activity: campaigning. As Kirchheimer discerned, the transformation of advanced industrial societies radically altered the context of parties’ strategic decision-making. In the area of election campaigning, parties confronted heightened incentives to approach the electorate with ‘persuasive’ rather than more traditional ‘mobilizing’ appeals and developed a greatly increased demand for reliable information about voter preferences. These shifts, in turn, had consequences for party organization, promoting the centralization and marginalization of individual members Kirchheimer associates with the catch-all party type. Through the lens of campaign change, then, we can observe the causal processes that unite the diverse features Kirchheimer links to his catch-all party — although our attention is also drawn to ways in which party development has not conformed to Kirchheimer’s expectations. This article illustrates these intersecting processes with a discussion of Labour and Conservative campaigns in Britain.
Providing Sexually Transmitted Disease Education and Risk Assessment to Disengaged Young Men Through Community Outreach
Authors: Johnson David , Harrison Patricia , Sidebottom Abbey
Published: August 2009

This study describes a community outreach project designed to increase access to reproductive health services to young African American men living in low-income urban neighborhoods. The project examined the effectiveness of providing community-based outreach and health education on increasing sexually transmitted disease (STD) screening. Outreach workers provided STD education and risk assessment in community settings, including street corners, parks, schools, and community centers. Data were recorded on outreach contacts, including client demographics, health education topics covered, and risk assessment results. Outreach workers conducted 9,701 contacts in a 176-week period. Most contacts (89%) were with African Americans, and most (84%) were with young men between 15 and 20 years old. Outreach workers discussed each health education item in their protocol at least 85% of the time and each risk assessment item at least 90% of the time. The majority of contacts (94%) reported being sexually active. Compared with the year prior to the project, actual STD testing of the target population doubled at the project clinics. This study suggests that going beyond traditional clinic-based testing to reach young, disenfranchised males constitutes a promising approach to reducing racial disparities in STD infections.
Marijuana: Use Among Young Males and Health Outcomes
Authors: Pujazon-Zazik Melissa , Park M. Jane
Published: August 2009

Marijuana is the most commonly used illicit drug by adolescents and young adults, with more males than females reporting marijuana use. The adolescent and young adult years represent a critical period for interventions to prevent marijuana use and abuse. This article reviews relevant literature, including trends in young males’ marijuana use and health effects of marijuana use. By most measures, there has been little net change in marijuana use among 12th graders and young adults since the 1990s. Despite males’ greater use, little research has examined gender differences in areas such as metabolism of marijuana and long-term impact of marijuana use. In many areas, including dental health, fertility, and respiratory function, research is either sparse or has yielded conflicting results. Similarly, research on marijuana’s carcinogenic effects has yielded conflicting results; however, a small but consistent literature indicates that marijuana use is linked to cancers unique to males. A stronger literature has identified an association between marijuana use and psychiatric problems. Clinical and program interventions for adolescents have potential to prevent marijuana use, as well as screen for and treat marijuana abuse. Improved research is needed, such as research with greater consistency in defining levels of use and greater emphasis on gender differences. Such research would help clinical and program interventions focus on those most at risk for adverse outcomes.
Intent to Remarry Among Chinese Elderly Widowers: An Oasis or an Abyss?
Authors: Chiu Marcus Y. L. , Ho Winnie W. N.
Published: August 2009

To investigate the intent to remarry and the predictors for such intent with psychosocial variables, this study used male interviewers to interview 180 Chinese elderly widowers. A structured questionnaire on singlehood and intent to remarry, which developed out of a pilot in-depth focus-group study, was used. Data were analyzed with nonparametric tests and logistic regression. The intent to remarry was predicted by younger age, inability to meet sexual needs, and dissatisfaction with widowhood. A total of 40% of these widowers who wanted a partner considered finding one from Mainland China. The sex-related findings were very different from previous studies on Chinese men. They showed that there should be professional support to help them to better adjust to late-life marriage. In addition, the study uncovered the need to expand the scope and enhance the sensitivity of current general practitioners and geriatric services to address issues beyond medical concerns.
Profiles of Urban, Low SES, African American Girls’ Attitudes Toward Science
Authors: Buck Gayle , Cook Kristin , Quigley Cassie , Eastwood Jennifer , Lucas Yvonne
Published: August 2009

The purpose of this study was to increase the science education community’s understanding of the experiences and needs of girls who cross the traditional categorical boundaries of gender, race and socioeconomic status in a manner that has left their needs and experience largely invisible. A first of several in a series, this study sought to explore how African American girls from low SES communities position themselves in science learning. We followed a mixed-methods sequential explanatory strategy, in which two data collection phases, qualitative following the quantitative, were employed to investigate 89 African-American girls’ personal orientations towards science learning. By using quantitative data from the Modified Attitudes toward Science Inventory to organize students into attitude profiles and then sequentially integrating the profile scores with year-long interview data, we found that the girls’ orientations towards science were best described in terms of definitions of science, importance of science, experiences with science, and success in science. Therefore, our mixed method analysis provided four personality orientations which linked success in school and experiences with science to confidence and importance of science and definitions of science to value/desire. In our efforts to decrease the achievement gap, we concluded there should be more emphasis on conceptual understanding and problem-solving skills, while still being cognizant of the danger of losing the connection between science and society which so often plagues achievement-focused efforts. Our continued efforts with this group of girls will center on these instructional techniques with the goal of addressing the needs of all science learners.
Are nicotine replacement therapy, varenicline or bupropion options for pregnant mothers to quit smoking? Effects on the respiratory system of the offspring
Authors: Maritz Gert S.
Published: August 2009

Nicotine occurs in tobacco smoke. It is a habit-forming substance and is prescribed by health professionals to assist smokers to quit smoking. It is rapidly absorbed from the lungs of smokers. It crosses the placenta and accumulates in the developing fetus. Nicotine induces formation of oxygen radicals and at the same time also reduces the antioxidant capacity of the lungs. Nicotine and the oxidants cause point mutations in the DNA molecule thereby changing the program that controls lung growth and maintenance of lung structure. The data available indicate that maternal nicotine exposure induces a persistent inhibition of glycolysis and a drastically increased AMP level. These metabolic changes are thought to contribute to the faster aging of the lungs of the offspring of mothers that are exposed to nicotine via the placenta and mother’s milk. The lungs of these animals are more susceptible to damage as shown by the gradual deterioration of the lung parenchyma. The rapid metabolic and structural aging of the lungs of the animals exposed to nicotine via the placenta and mother’s milk, and thus during phases of lung development characterized by rapid cell division, is likely due to ‘programming’ induced by nicotine. Since varenicline, a partial nicotine agonist, has basically the same structure as nicotine, and also binds to acetylcholine receptors in competition with nicotine (but with largely the same effect), it is not advisable to use nicotine or varenicline during gestation and lactation. Furthermore, the use of individual vitamin supplements is also not advisable because of the negative impact on the program that controls maintenance of lung structural and functional integrity and aging. A more appropriate smoking cessation program will also include a mixture of antioxidant nutrients such as in tomato juice.
Pelvic Congestion Syndrome and Its Relationship to Varices of the Lower Extremities
Authors: Wheelock Kathleen
Published: August 2009

Pelvic congestion syndrome occurs when the ovarian veins are incompetent; it is a common cause of chronic pelvic pain, which often goes undiagnosed and is also a cause of lower extremity varicose veins. Through the review of pelvic venous anatomy, the complexity of pelvic congestion syndrome and the relationships that create this chain reaction of symptoms can be diagnosed, and a holistic course of treatment can be applied.
Acute dasatinib exposure commits Bcr-Abl–dependent cells to apoptosis
Authors: Snead Jennifer L. , O'Hare Thomas , Adrian Lauren T. , Eide Christopher A. , Lange Thoralf , Druker Brian J. , Deininger Michael W.
Published: August 2009

Pioneering work with the Bcr-Abl inhibitor, imatinib, demonstrated a requirement for constant Bcr-Abl inhibition to achieve maximal therapeutic benefit in treating chronic myeloid leukemia (CML), establishing a paradigm that has guided further drug development for this disease. Surprisingly, the second-generation Bcr-Abl inhibitor, dasatinib, was reported to be clinically effective with once-daily dosing, despite a short (3- to 5-hour) plasma half-life. Consistent with this observation, dasatinib treatment of progenitor cells from chronic-phase CML patients for 4 hours, followed by washout, or continuously for 72 hours both resulted in an induction of apoptosis and a reduction in the number of clonogenic cells. Such acute treatments with clinically achievable dasatinib concentrations also irreversibly committed Bcr-Abl+ CML cell lines to apoptotic cell death. Potent transient Bcr-Abl inhibition using the alternative inhibitor, nilotinib, also resulted in cell death. These findings demonstrate that in vitro assays designed to model in vivo pharmacokinetics can predict clinical efficacy. Furthermore, they challenge the widely held notion that continuous target inhibition is required for optimal efficacy of kinase inhibitors.
Association between beta2-glycoprotein I plasma levels and the risk of myocardial infarction in older men
Authors: de Laat Bas , de Groot Philip G. , Derksen Ronald H. W. M. , Urbanus Rolf T. , Mertens Koen , Rosendaal Frits R. , Doggen Carine J. M.
Published: August 2009

von Willebrand factor (VWF) serves as adhesive surface for platelets to adhere to the vessel wall. We have recently found that beta2-glycoprotein I is able to inhibit platelet binding to VWF, indicating a role in the pathophysiology of arterial thrombosis. In the present study, we investigated whether differences in beta2-glycoprotein I plasma levels influence the risk of myocardial infarction. We have measured beta2-glycoprotein I and VWF antigen levels in 539 men with a first myocardial infarction and in 611 control subjects. Although we did not find a profound effect of beta2-glycoprotein I plasma levels on myocardial infarction in the overall population, we found a dose-dependent protective effect of increasing beta2-glycoprotein I plasma levels on myocardial infarction in men 60 years and older. In this age group, we found an odds ratio of 0.41 (95% confidence interval, 0.22-0.74) for high beta2-glycoprotein I levels compared with low levels. High plasma levels of beta2-glycoprotein I remained protective for myocardial infarction despite high levels of VWF. To conclude, high circulating levels of beta2-glycoprotein I appeared to be associated with a reduced risk of myocardial infarction in elderly men. In vivo experiments are needed to investigate the exact contribution of beta2-glycoprotein I on the pathophysiology of myocardial infarction.
Macrophages are an abundant component of myeloma microenvironment and protect myeloma cells from chemotherapy drug–induced apoptosis
Authors: Zheng Yuhuan , Cai Zhen , Wang Siqing , Zhang Xiang , Qian Jianfei , Hong Sungyoul , Li Haiyan , Wang Michael , Yang Jing , Yi Qing
Published: August 2009

Multiple myeloma remains an incurable disease. One of the major problems is that myeloma cells develop drug resistance on interaction with bone marrow stromal cells. In this study, we examined the effects of macrophages (Mφs), a type of stromal cells, on myeloma cell survival and response to chemotherapy. We showed that Mφ, in particular tumor-associated Mφ, is a protector of myeloma cells. The protective effect was dependent on direct contact between Mφs and myeloma cells. Mφs protected both myeloma cell lines and primary myeloma cells from spontaneous and chemotherapy drug-induced apoptosis by attenuating the activation and cleavage of caspase-dependent apoptotic signaling. These findings are clinically relevant because we found that CD68+ Mφs heavily infiltrate the bone marrow of patients with myeloma but not the bone marrow of control patients. Thus, our results indicate that Mφs may contribute to myeloma cell survival and resistance to chemotherapeutic treatment in vivo.
Phase 3 randomized, placebo-controlled, double-blind study of high-dose continuous infusion cytarabine alone or with laromustine (VNP40101M) in patients with acute myeloid leukemia in first relapse
Authors: Giles Francis , Vey Norbert , DeAngelo Daniel , Seiter Karen , Stock Wendy , Stuart Robert , Boskovic Darinka , Pigneux Arnaud , Tallman Martin , Brandwein Joseph , Kell Jonathan , Robak Tadeusz , Staib Peter , Thomas Xavier , Cahill Ann , Albitar Maher , O'Brien Susan
Published: August 2009

Laromustine is a sulfonylhdrazine alkylator with significant antileukemia activity. An international, randomized (2:1), double-blind, placebo-controlled study was conducted to compare complete remission (CR) rates and overall survival (OS) in patients with first relapse acute myeloid leukemia (AML) treated with laromustine and high-dose cytarabine (HDAC) versus HDAC/placebo. Patients received 1.5 g/m2 per day cytarabine continuous infusion for 3 days and laromustine 600 mg/m2 (n = 177) or placebo (n = 86) on day 2. Patients in CR received consolidation with laromustine/HDAC or HDAC/placebo as per initial randomization. After interim analysis at 50% enrollment, the Data Safety Monitoring Board (DSMB) expressed concern that any advantage in CR would be compromised by the observed on-study mortality, and enrollment was held. The CR rate was significantly higher for the laromustine/HDAC group (35% vs 19%, P = .005). However, the 30-day mortality rate and median progression-free survival were significantly worse in this group compared with HDAC/placebo (11% vs 2%; P = .016; 54 days vs 34; P = .002). OS and median response durations were similar in both groups. Laromustine/HDAC induced significantly more CR than HDAC/placebo, but OS was not improved due to mortality associated with myelosuppression and its sequelae. The DSMB subsequently approved a revised protocol with laromustine dose reduction and recombinant growth factor support. The study was registered as NCT00112554 at http://www.clinicaltrials.gov.
dictyExpress: a Dictyostelium discoideum gene expression database with an explorative data analysis web-based interface
Authors: Rot Gregor , Parikh Anup , Curk Tomaz , Kuspa Adam , Shaulsky Gad , Zupan Blaz
Published: August 2009

Background

Bioinformatics often leverages on recent advancements in computer science to support biologists in their scientific discovery process. Such efforts include the development of easy-to-use web interfaces to biomedical databases. Recent advancements in interactive web technologies require us to rethink the standard submit-and-wait paradigm, and craft bioinformatics web applications that share analytical and interactive power with their desktop relatives, while retaining simplicity and availability.

Results

We have developed dictyExpress, a web application that features a graphical, highly interactive explorative interface to our database that consists of more than 1000 Dictyostelium discoideum gene expression experiments. In dictyExpress, the user can select experiments and genes, perform gene clustering, view gene expression profiles across time, view gene co-expression networks, perform analyses of Gene Ontology term enrichment, and simultaneously display expression profiles for a selected gene in various experiments. Most importantly, these tasks are achieved through web applications whose components are seamlessly interlinked and immediately respond to events triggered by the user, thus providing a powerful explorative data analysis environment.

Conclusion

dictyExpress is a precursor for a new generation of web-based bioinformatics applications with simple but powerful interactive interfaces that resemble that of the modern desktop. While dictyExpress serves mainly the Dictyostelium research community, it is relatively easy to adapt it to other datasets. We propose that the design ideas behind dictyExpress will influence the development of similar applications for other model organisms.
Adherent-invasive Escherichia coli, strain LF82 disrupts apical junctional complexes in polarized epithelia
Authors: Wine Eytan , Ossa Juan C , Gray-Owen Scott D , Sherman Philip M
Published: August 2009

Background

Although bacteria are implicated in the pathogenesis of chronic inflammatory bowel diseases (IBD), mechanisms of intestinal injury and immune activation remain unclear. Identification of adherent-invasive Escherichia coli (AIEC) strains in IBD patients offers an opportunity to characterize the pathogenesis of microbial-induced intestinal inflammation in IBD. Previous studies have focused on the invasive phenotype of AIEC and the ability to replicate and survive in phagocytes. However, the precise mechanisms by which these newly identified microbes penetrate the epithelial lining remain to be clarified. Therefore, the aim of this study was to delineate the effects of AIEC, strain LF82 (serotype O83:H1) on model polarized epithelial monolayers as a contributor to intestinal injury in IBD.

Results

Infection of T84 and Madin-Darby Canine Kidney-I polarized epithelial cell monolayers with AIEC, strain LF82 led to a reduction in transepithelial electrical resistance and increased macromolecular (10 kilodalton dextran) flux. Basolateral AIEC infection resulted in more severe disruption of the epithelial barrier. Increased permeability was accompanied by a redistribution of the tight junction adaptor protein, zonula occludens-1, demonstrated by confocal microscopy and formation of gaps between cells, as shown by transmission electron microscopy. After 4 h of infection of intestine 407 cells, bacteria replicated in the cell cytoplasm and were enclosed in membrane-bound vesicles positive for the late endosomal marker, LAMP1.

Conclusion

These findings indicate that AIEC, strain LF82 disrupts the integrity of the polarized epithelial cell barrier. This disruption enables bacteria to penetrate into the epithelium and replicate in the host cell cytoplasm. These findings provide important links between microbes related to IBD, the intestinal epithelial cell barrier and disease pathogenesis.
Expression of the glioma-associated oncogene homolog (GLI) 1in human breast cancer is associated with unfavourable overall survival
Authors: ten Haaf Anette , Bektas Nuran , von Serenyi Sonja , Losen Inge , Arweiler Elfriede Christel , Hartmann Arndt , Knüchel Ruth , Dahl Edgar
Published: August 2009

Background

The transcription factor GLI1, a member of the GLI subfamily of Krüppel-like zinc finger proteins is involved in signal transduction within the hedgehog pathway. Aberrant hedgehog signalling has been implicated in the development of different human tumour entities such as colon and lung cancer and increased GLI1 expression has been found in these tumour entities as well. In this study we questioned whether GLI1 expression might also be important in human breast cancer development. Furthermore we correlated GLI1 expression with histopathological and clinical data to evaluate whether GLI1 could represent a new prognostic marker in breast cancer treatment.

Methods

Applying semiquantitative realtime PCR analysis and immunohistochemistry (IHC) GLI1 expression was analysed in human invasive breast carcinomas (n = 229) in comparison to normal human breast tissues (n = 58). GLI1 mRNA expression was furthermore analysed in a set of normal (n = 3) and tumourous breast cell lines (n = 8). IHC data were statistically interpreted using SPSS version 14.0.

Results

Initial analysis of GLI1 mRNA expression in a small cohort of (n = 5) human matched normal and tumourous breast tissues showed first tendency towards GLI1 overexpression in human breast cancers. However only a small sample number was included into these analyses and values for GLI1 overexpression were statistically not significant (P = 0.251, two-tailed Mann-Whitney U-test). On protein level, nuclear GLI1 expression in breast cancer cells was clearly more abundant than in normal breast epithelial cells (P = 0.008, two-tailed Mann-Whitney U-test) and increased expression of GLI1 protein in breast tumours significantly correlated with unfavourable overall survival (P = 0.019), but also with higher tumour stage (P < 0.001) and an increased number of tumour-positive axillar lymph nodes (P = 0.027). Interestingly, a highly significant correlation was found between GLI1 expression and the expression of SHH, a central upstream molecule of the hedgehog pathway that was previously analysed on the same tissue microarray.

Conclusion

Our study presents a systematic expression analysis of GLI1 in human breast cancer. Elevated levels of GLI1 protein in human breast cancer are associated with unfavourable prognosis and progressive stages of disease. Thus GLI1 protein expression measured e.g. by an IHC based scoring system might have an implication in future multi-marker panels for human breast cancer prognosis or molecular sub typing. The highly significant correlation between SHH and GLI1 expression characterises GLI1 as a potential functional downstream target of the hedgehog signalling pathway in human breast cancer as well. Furthermore, our study indicates that altered hedgehog signalling may represent a key disease pathway in the progression of human breast cancer.
Directed evolution of a filamentous fungus for thermotolerance
Authors: de Crecy Eudes , Jaronski Stefan , Lyons Benjamin , Lyons Thomas J , Keyhani Nemat O
Published: August 2009

Background

Filamentous fungi are the most widely used eukaryotic biocatalysts in industrial and chemical applications. Consequently, there is tremendous interest in methodology that can use the power of genetics to develop strains with improved performance. For example, Metarhizium anisopliae is a broad host range entomopathogenic fungus currently under intensive investigation as a biologically based alternative to chemical pesticides. However, it use is limited by the relatively low tolerance of this species to abiotic stresses such as heat, with most strains displaying little to no growth between 35–37°C. In this study, we used a newly developed automated continuous culture method called the Evolugator™, which takes advantage of a natural selection-adaptation strategy, to select for thermotolerant variants of M. anisopliae strain 2575 displaying robust growth at 37°C.

Results

Over a 4 month time course, 22 cycles of growth and dilution were used to select 2 thermotolerant variants of M. anisopliae. Both variants displayed robust growth at 36.5°C, whereas only one was able to grow at 37°C. Insect bioassays using Melanoplus sanguinipes (grasshoppers) were also performed to determine if thermotolerant variants of M. anisopliae retained entomopathogenicity. Assays confirmed that thermotolerant variants were, indeed, entomopathogenic, albeit with complex alterations in virulence parameters such as lethal dose responses (LD50) and median survival times (ST50).

Conclusion

We report the experimental evolution of a filamentous fungus via the novel application of a powerful new continuous culture device. This is the first example of using continuous culture to select for complex phenotypes such as thermotolerance. Temperature adapted variants of the insect-pathogenic, filamentous fungus M. anisopliae were isolated and demonstrated to show vigorous growth at a temperature that is inhibitory for the parent strain. Insect virulence assays confirmed that pathogenicity can be retained during the selection process. In principle, this technology can be used to adapt filamentous fungi to virtually any environmental condition including abiotic stress and growth substrate utilization.
Respiratory maneuvers in echocardiography: a review of clinical applications
Authors: Ginghina Carmen , Beladan Carmen C , Iancu Madalina , Calin Andreea , Popescu Bogdan A
Published: August 2009

During echocardiographic examination, respiration induces cyclic physiological changes of intracardiac haemodynamics, causing normal variations of the right and left ventricle Doppler inflows and outflows and physiological variation of extracardiac flows. The respiration related hemodynamic variation in intra and extracardiac flows may be utilized in the echocardiography laboratory to aid diagnosis in different pathological states. Nevertheless, physiologic respiratory phases can cause excessive translational motion of cardiac structures, lowering 2D image quality and interfering with optimal Doppler interrogation of flows or tissue motion.

This review focuses on the impact of normal respiratory cycle and provocative respiratory maneuvers in echocardiographic examination, both in physiological and pathological states, emphasizing their applications in specific clinical situations.
Comparison of follicular fluid and serum levels of Inhibin A and Inhibin B with calculated indices used as predictive markers of ovarian hyperstimulation syndrome in IVF patients
Authors: Moos Jiri , Rezabek Karel , Filova Vanda , Moosova Martina , Pavelkova Jana , Peknicova Jana
Published: August 2009

Background

Ovarian Hyperstimulation Syndrome (OHSS) is a severe health complication observed in some patients undergoing hormonal stimulation during IVF. Presence of OHSS is often associated with a high count of growing follicles responding to FSH hyperstimulation. However, the number of responding follicles may not be sufficient enough to predict the onset and severity of OHSS. The aim of this study was to find whether follicular fluid (FF) and serum concentrations of Inhibin A and Inhibin B in patients undergoing IVF treatment may serve as a predictor of OHSS status independent of the growing follicles count.

Methods

Serum and follicular fluid of fifty-three women undertaking the IVF program were separated into four groups according to their OHSS status and growing follicles count and analyzed for serum and FF concentrations of Inhibin A and Inhibin B. The resulting data were combined with clinical and demographic data to calculate indices independent of the growing follicles count.

Results

Serum Inhibin A and Inhibin B concentrations showed no significant difference between the severe OHSS group and the control group without OHSS. Moreover, the serum concentrations of Inhibin A and Inhibin B were strongly correlated with the growing follicles count. Their concentrations in the high responders group (>18 follicles) were significantly higher (p < 0.00001, p < 0.0001) when compared with normal and low responders (<18 follicles). To suppress the dependence on the growing follicle count, three indices were constructed and calculated. The best association with OHSS status and independence of the growing follicle count was achieved by using the Inhibin B TFF/SBM index calculated as follows: [concentration in FF] × [growing follicle count]/[concentration in serum] × [body mass]. The Inhibin B TFF/SBM index showed a clear difference (p = 0,00433) between the group with severe OHSS and the control group, while showing no apparent correlation with the growing follicle count.

Conclusion

These observations demonstrated that while neither serum nor FF concentrations of Inhibin A nor Inhibin B can be used as an OHSS predictor independent of the growing follicle count, calculated indices may meet the criteria.
Prolactin signaling through the short isoform of the mouse prolactin receptor regulates DNA binding of specific transcription factors, often with opposite effects in different reproductive issues
Authors: Devi Y Sangeeta , Shehu Aurora , Halperin Julia , Stocco Carlos , Le Jamie , Seibold Anita M , Gibori Geula
Published: August 2009

Background

It has been well established that prolactin (PRL) signals through the long form of its receptor (PRL-RL) and activates the Jak/Stat pathway for transcription of PRL target genes. However, signaling pathways mediated through the short PRL-R isoform (PRL-RS) remains controversial. Our recent finding that PRL signaling through PRL-RS represses two transcription factors critical for follicular development lead us to examine other putative PRL/PRL-RS target transcription factors in the decidua and ovary, two well-known target tissues of PRL action in reproduction.

Methods

In this investigation we used mice expressing PRL-RS on a PRL-R knockout background and a combo protein/DNA array to study the transcription factors regulated by PRL through PRL-RS only.

Results

We show that PRL activation of the PRL-RS receptor either stimulates or inhibits the DNA binding activity of a substantial number of transcription factors in the decidua as well as ovary. We found few transcription factors to be similarly regulated in both tissues, while most transcription factors are oppositely regulated by PRL in the decidua and ovary. In addition, some transcription factors are regulated by PRL only in the ovary or only in the decidua. Several of these transcription factors are involved in physiological pathways known to be regulated by PRL while others are novel.

Conclusion

Our results clearly indicate that PRL does signal through PRL-RS in the decidua as well as the ovary, independently of PRL-RL, and activates/represses transcription factors in a tissue specific manner. This is the first report showing PRL/PRL-RS regulation of specific transcription factors. Many of these transcription factors were not previously known to be PRL targets, suggesting novel physiological roles for this hormone.
Identification of HLA-A*2402-restricted HCMV immediate early-1 (IE-1) epitopes as targets for CD8+ HCMV-specific cytotoxic T lymphocytes
Authors: Lim Jong-Baeck , Kim Hyun Ok , Jeong Seok Hoon , Ha Joo Eun , Jang Sunphil , Lee Sang-Guk , Lee Kyungwon , Stroncek David
Published: August 2009

Background

To identify novel HLA-A*2402-restricted human cytomegalovirus (HCMV) immediate early-1 (IE-1) epitopes for adoptive immunotherapy, we explored 120 overlapping 15-amino acid spanning IE-1.

Methods

These peptides were screened by measuring the frequency of polyclonal CD8+ T cells producing intracellular interferon-γ (IFN-γ) using flow cytometry and the epitopes were validated with a HCMV-infected target Cr release cytotoxicity assay.

Results

Initial screening was performed with 12 mini-pools of 10 consecutive peptides made from 120 overlapping peptides15-amino acids in length that spanned IE-1. When peripheral blood mononuclear cells (PBMCs) from HLA-A*2402 HCMV-seropositive donors were sensitized with each of the 12 mini-pools, mini-pools 1 and 2 induced the highest frequency of CD8+ cytotoxic T lymphocytes (CTLs) producing IFN-γ. When PBMCs were stimulated with each of the twenty peptides belonging to mini-pools 1 and 2, peptides IE-11–15MESSAKRKMDPDNPD and IE-15–19AKRKMDPDNPDEGPS induced the greatest quantities of IFN-γ production and cytotoxicity of HLA-matched HCMV-infected fibroblasts. To determine the exact HLA-A*2402-restricted epitopes within the two IE-1 proteins, we synthesized a total of twenty-one overlapping 9- or 10 amino acid peptides spanning IE-11–15and IE-15–19. Peptide IE-13–12SSAKRKMDPD induced the greatest quantities of IFN-γ production and target cell killing by CD8+ CTLs.

Conclusion

HCMV IE-13–12SSAKRKMDPD is a HLA-A*2402-restricted HCMV IE-1 epitope that can serve as a common target for CD8+ HCMV-specific CTLs.
A cross sectional study of prevalence, risk factors, population attributable fractions and pathology for foot and limb lesions in preweaning piglets on commercial farms in England
Authors: KilBride Amy L , Gillman Claire E , Ossent Pete , Green Laura E
Published: August 2009

Background

In a cross sectional study of 88 indoor and outdoor English pig farms, the prevalence of foot and limb lesions in 2843 preweaning piglets aged 1–4 weeks from 304 litters was recorded. The environmental risks for the prevalence of lesions and population attributable fractions were calculated. The risks for lesions in piglets were compared with those for limb and body lesions in their mothers. A small number of piglets with each type of lesion were examinedpost mortemto elucidate the pathology of the clinical lesions observed.

Results

The prevalence of sole bruising, sole erosion, skin abrasion and swollen joints or claws in 2843 piglets was 49.4% (1404), 15.5% (441), 43.6% (1240) and 4.7% (143) respectively. The prevalence of all foot and limb lesions was higher in indoor housed piglets than in outdoor housed piglets. The prevalence of sole bruising (OR 0.3) and skin abrasion (OR 0.6) decreased with each week of age from 1–4 weeks, but there was no significant association between piglet age and the prevalence of sole erosion or swollen joints and claws. There was an increased prevalence of sole bruising (OR 3.0) and swollen joints or claws (OR 3.0) and a decreased prevalence of skin abrasion (OR 0.3, piglets ≤ 1-week old), in piglets housed on slatted floors, compared with those on solid concrete floors with bedding. There was an increased risk of sole erosion associated with piglets housed on partly slatted floors with no bedding (OR 2.4) and partly slatted floors with small amounts of bedding (OR 2.9) compared with piglets housed on solid concrete floors with bedding in all areas of the pen.Post mortemexamination of feet with lesions indicated that internal pathological changes were frequently more severe than the degree of external damage suggested.

Conclusion

Piglets housed outdoors had a very low prevalence of foot and limb injuries. Indoors, no one floor type was ideal to minimise all piglet foot and limb injuries and the flooring requirements of sows differed from those of piglets.
Accurate poststack acoustic-impedance inversion by well-log calibration
Authors: Morozov Igor B. , Ma Jinfeng
Published: August 2009

The seismic-impedance inversion problem is underconstrained inherently and does not allow the use of rigorous joint inversion. In the absence of a true inverse, a reliable solution free from subjective parameters can be obtained by defining a set of physical constraints that should be satisfied by the resulting images. A method for constructing synthetic logs is proposed that explicitly and accurately satisfies (1) the convolutional equation, (2) time-depth constraints of the seismic data, (3) a background low-frequency model from logs or seismic/geologic interpretation, and (4) spectral amplitudes and geostatistical information from spatially interpolated well logs. The resulting synthetic log sections or volumes are interpretable in standard ways. Unlike broadly used joint-inversion algorithms, the method contains no subjectively selected user parameters, utilizes the log data more completely, and assesses intermediate results. The procedure is simple and tolerant to noise, and it leads to higher-resolution images. Separating the seismic and subseismic frequency bands also simplifies data processing for acoustic-impedance (AI) inversion. For example, zero-phase deconvolution and true-amplitude processing of seismic data are not required and are included automatically in this method. The approach is applicable to 2D and 3D data sets and to multiple pre- and poststack seismic attributes. It has been tested on inversions for AI and true-amplitude reflectivity using 2D synthetic and real-data examples.
Airborne gravity gradiometry: Terrain corrections and elevation error
Authors: Dransfield Mark , Zeng Yi
Published: August 2009

Terrain corrections for airborne gravity gradiometry data are calculated from a digital elevation model (DEM) grid. The relative proximity of the terrain to the gravity gradiometer and the relative magnitude of the density contrast often result in a terrain correction that is larger than the geologic signal of interest in resource exploration. Residual errors in the terrain correction can lead to errors in data interpretation. Such errors may emerge from a DEM that is too coarsely sampled, errors in the density assumed in the calculations, elevation errors in the DEM, or navigation errors in the aircraft position. Simple mathematical terrains lead to the heuristic proposition that terrain-correction errors from elevation errors in the DEM are linear in the elevation error but follow an inverse power law in the ground clearance of the aircraft. Simulations of the effect of elevation error on terrain-correction error over four measured DEMs support this proposition. This power-law relation may be used in selecting an optimum survey flying height over a known terrain, given a desired terrain-correction error.
Casing effects in the sea-to-borehole electromagnetic method
Authors: Kong F. N. , Roth F. , Olsen P. A. , Stalheim S. O.
Published: August 2009

The sea-to-borehole controlled source electromagnetic (CSEM) method has the potential of becoming a useful new exploration and monitoring technique. Modeling shows that the electromagnetic fields at reservoir depths can be 1-2 orders of magnitude larger than those on the seabed, thus opening up the possibility to see deeper or detect and monitor smaller targets. In the case of measurements inside metal casing, the use of reciprocity to interchange the source and receiver allows the casing conductor to be considered as an antenna. This antenna is excited by a source inside the casing, which induces a current flowing along the casing conductor. Based on the current distribution, a casing dipole moment and an equivalent length were derived for the transverse magnetic (TM) mode. In the frequency range of interest (0.1–10 Hz), the increase in field strength (resulting fromsea-to-borehole measurements) is not lost to the casing attenuation for either the TM or the transverse electric (TE) mode. The ratio of the dipole moment of the casing antenna to that of the source current can be considered as the casing attenuation effect. This ratio is not far from unity for frequencies smaller than [Formula: see text], but it decreases rapidly with frequency. The equivalent length of the casing antenna can be of the order of a few hundred meters. As a consequence, the field distribution changes significantly in the presence of casing, and the vertical resolution of TM measurements is degraded. The horizontal resolution is not degraded significantly. Measurement of the TM mode has the advantage of being sensitive to thin resistive targets and being less affected by sea-air interactions in shallow water. The TE mode measurement, having a localized casing effect, contributes valuable information about the background resistivity.
Activity of Fulvestrant 500 mg Versus Anastrozole 1 mg As First-Line Treatment for Advanced Breast Cancer: Results From the FIRST Study
Authors: Robertson John F.R. , Llombart-Cussac Antonio , Rolski Janusz , Feltl David , Dewar John , Macpherson Euan , Lindemann Justin , Ellis Matthew J.
Published: August 2009

Purpose

To compare the clinical activity of the pure antiestrogen fulvestrant at 500 mg/mo (double the approved dose) with the aromatase inhibitor anastrozole as first-line endocrine therapy for advanced hormone receptor–positive breast cancer in postmenopausal women.

Patients and Methods

FIRST (Fulvestrant First-Line Study Comparing Endocrine Treatments) is a phase II, randomized, open-label, multicenter study of a fulvestrant high-dose (HD) regimen (500 mg/mo plus 500 mg on day 14 of month 1) versus anastrozole (1 mg/d). The primary efficacy end point was clinical benefit rate (CBR), defined as the proportion of patients experiencing an objective response (OR) or stable disease for ≥ 24 weeks. The primary analysis was performed 6 months after the last patient was randomly assigned.

Results

CBR was similar for fulvestrant HD (n = 102) and anastrozole (n = 103), 72.5% v 67.0%, respectively (odds ratio, 1.30; 95% CI, 0.72 to 2.38; P = .386). Objective response rate (ORR) was also similar between treatments: fulvestrant HD, 36.0%; anastrozole, 35.5%. Time to progression (TTP) was significantly longer for fulvestrant versus anastrozole (median TTP not reached for fulvestrant HD v 12.5 months for anastrozole; hazard ratio, 0.63; 95% CI, 0.39 to 1.00; P = .0496). Duration of OR and CB also numerically favored fulvestrant HD. Both treatments were well tolerated, with no significant differences in the incidence of prespecified adverse events.

Conclusion

First-line fulvestrant HD was at least as effective as anastrozole for CBR and ORR and was associated with significantly longer TTP. Fulvestrant HD was generally well tolerated, with a safety profile similar to that of anastrozole.