bluuebunny/crossref_metadata_2025 huggingface dataset:
Executive functions are modulated by the context of dual language use: diglossic, bilingual and monolingual older adults
Authors: Alrwaita Najla , Houston-Price Carmel , Meteyard Lotte , Voits Toms , Pliatsikas Christos
Published: February 2023

Studies investigating the role of dual language use in modulating executive functions have reported mixed results, with some studies reporting benefits in older adults. These studies typically focus on bilingual settings, while the role of dual language use in diglossic settings is rarely investigated. In diglossia, the two language varieties are separated by context, making it an ideal test case for the effects on cognition of Single Language Contexts, as defined by the Adaptive Control Hypothesis (Green & Abutalebi, 2013). We compare the performances of three groups of older adults, Arab diglossics (n = 28), bilinguals (n = 29), and monolinguals (n = 41), on the Flanker and Stroop tasks, measuring inhibition abilities, and the Color-shape task, measuring switching abilities. We report a diglossic benefit in inhibition as measured by the Flanker task only, and no benefits for the bilingual group. These findings are discussed with reference to conversational contexts in dual language use.
Prospective memory in bilinguals: Recalling future intentions in first and second language contexts
Authors: López-Rojas Cristina , Marful Alejandra , Pérez Ana I. , Bajo M. Teresa
Published: February 2023

Recalling future intentions (i.e., prospective memory, PM) plays an essential role in everyday life, but sometimes, if the person is involved in a demanding ongoing task, PM is unsuccessful. This is especially relevant for bilinguals who, in many situations, have to recall intentions while performing a task in their second language (L2). Our aim was to explore whether PM is modulated by the linguistic context in which PM takes place. In this study, bilinguals performed a PM task in their first (L1) or second language (L2). We also manipulated the demands of the ongoing task (early/late updating) and the PM cue (focal/non-focal). In general, results showed an overall impairment in the recall of future intentions when the task was performed in L2. This impairment was especially evident in the more demanding conditions, suggesting that increments in attentional demands due to L2 processing hinder the processes required for prospective remembering.
Lexical production and innovation in child and adult Russian Heritage speakers dominant in English and Hebrew
Authors: Fridman Clara , Meir Natalia
Published: February 2023

The present study investigated lexical production and innovation of 202 participants across six groups: child and adult heritage speakers of Russian, dominant in Hebrew or American English, and monolingual Russian-speaking children and adults. Understanding quantitative performance across these six groups was intended to provide a comprehensive perspective on heritage language (HL) development, while comparing the participants’ qualitative non-target response patterns would elucidate the organization of the HL lexicon. We assessed the production of Russian nouns and verbs using a naming task. We then considered the effects of input at the societal and lexical levels (focusing on word frequency and age of acquisition). Our findings are discussed in terms of accounts of HL developmental trajectories: monolingual-like trajectory, frozen lexical development, attrition, and new language variety in a contact situation. The results presented no evidence for attrition, while elements of the other three trajectories were found in our quantitative and qualitative analyses.
Mixed language processing increases cross-language phonetic transfer in Bengali–English bilinguals
Authors: Mitra Auromita , Dutta Indranil
Published: February 2023

This study investigates the phonetic outcome of mixed-language speech in Bengali and Indian English, towards understanding cross-language interaction in highly proficient bilinguals. We compare spectral properties of L2 vowels [æ] (common to L1, L2) and [ʌ] (absent in L1) in code-switched (mixed) vs. nonswitched productions. Results reveal asymmetrical shifts in both vowels during mixed productions, towards related L1 categories. We interpret this as a temporary increase in cross-language phonetic interaction during mixed-language use, leading to a shift towards L1 norms, evidencing transfer effects on L2 vowels. We elicit mixed productions through two tasks: cued picture-naming and code-switching, to assess if experimental paradigm independently influences the behavior under study. Results reveal parallel patterns, but small magnitude differences, across paradigms. We discuss these findings in light of recent proposals about asymmetries in short-term phonetic interaction, postulated discursive factors in code-switching, and the issue of comparability between paradigms in transfer studies.
Family caregivers’ experience of communication with nursing home staff from admission to end of life during the COVID-19 pandemic: A qualitative study employing a transitional perspective
Authors: Gonella Silvia , Cornally Nicola , Antal Alexandra , Tambone Sara , Martin Peter , Dimonte Valerio , Di Giulio Paola
Published: February 2023

Objectives

Family caregivers’ (FCs) caregiving in nursing home (NH) moves across 3 main phases: transitioning relatives to long-term care, worsening of a relative’s conditions, and end of life; each phase brings specific challenges that FCs must confront. Moreover, during the COVID-19 pandemic, strict mandatory visitor restrictions affected communication modalities. This study explored FCs’ experience of communication with NH staff during the COVID-19 pandemic from admission to end of life.

Methods

A descriptive qualitative study with inductive content analysis was performed in 7 Italian NHs from May to June 2021. NH managers purposively identified 25 FCs at different phases of their caregiving trajectory: transitional (i.e., admission in the previous 8 weeks, n = 8), deterioration-in-condition (i.e., acknowledged changes in care needs of their relative after trigger events, n = 10), and end-of-life phase (i.e., death expected in the next weeks or a few months, n = 7), who were interviewed.

Results

Regardless the phase of caregiving trajectory, what mattered most to FCs was the opportunity to have regular and sensitive discussions with health-care professionals. The need of in-person communication increased nearing death. The COVID-19 pandemic enhanced FCs’ need to interact with health-care professionals they trusted. Knowledge of residents’ preferences mitigated FCs’ turbulent emotions throughout the overall caregiving trajectory.

Significance of results

Findings suggest that in-person contacts should be prioritized and facilitated when possible, particularly at the end of life; nonetheless, meaningful communication can occur also through remote modalities. Investments in training health-care professionals about effective long-distance communication and supportive skills can help trusting relationships to be established. Open discussions about residents’ care preferences should be encouraged.
Magnetic field dissipation in nucleonic neutron star cores
Authors: Ofengeim Dmitry D. , Gusakov Mikhail E. , Potekhin Alexander Y.
Published: February 2023

We study the long-term heating due to magnetic field decay in the core of neutron star. Two cases for the nucleonic core are considered: normal and strongly superconducting. We give simple scaling relations (depending on the internal stellar temperature and the averaged magnetic field in the core) to estimate the magnetic field decay rate for the most important dissipation processes. Comparison to properties of observed neutron stars suggests that heating due to the magnetic field decay is (at least partially) responsible for the thermal states of middle-aged magnetars and highly-magnetized isolated neutron stars with ages of 1 — 10 Myr.
Novel Clues to the Physics of Magnetars as Probed with Detailed Pulse-Timing Studies
Authors: Makishima Kazuo
Published: February 2023

Broadband X-ray data of five magnetars show that their hard X-ray pulses suffer periodic phase modulations, at a period ∼ 104 times their pulse period. The phenomenon is interpreted as a result of free precession of neutron stars that are prolately deformed to an asphericity of ∼ 10−4, by the magnetic stress of toroidal fields reaching ∼ 1016 G. The behavior is absent in their soft X-ray pulses, probably due to a higher emission symmetry. The ultra-high toroidal fields, considered common to magnetars, may persist longer than their dipole fields.
Magnetic Field Evolution in the Crust of Neutron Stars: Crust Failure and Plastic Flow
Authors: Gourgouliatos Konstantinos N.
Published: February 2023

The evolution of the magnetic field in neutron star crusts because of the Hall effect has received significant attention over the last two decades, which is strongly justified because of the dominance of this effect in highly magnetised neutron stars. However, the applicability of the Hall effect is based on the assumption that the crust does not fail and sustains its rigidity. This assumption can be violated for substantially strong magnetic fields. If this is the case, the evolution of the magnetic field is described by a different set of equations, which include the effects of a non-rigid crust. In this talk, after a brief review of the main characteristic of the Hall evolution, I will discuss the impact a plastic flow of the crust has on the magnetic field, studying axisymmetric models. Moreover, the way the crust fails impacts the overall evolution, with major differences appearing if the failure is local, intermediate or global. Quite remarkably, crustal failure and plasticity do not annul the Hall effect, and under certain circumstances they may even lead to a more dramatic evolution. I will discuss the impact of these effects in the context of neutron star timing behaviour, with special focus on timing noise, outbursts and glitches.
Compact Remnant Constraints on the Core-Collapse Engine
Authors: Fryer Chris L.
Published: February 2023

The convection-enhanced neutrino-driven supernova engine’s success in explaining a myriad of supernova properties has set it as the standard engine behind supernova. However, due to the success of rotationally-powered engines in explaining astrophysical transients like gamma-ray bursts, these engines have been revived as possible drivers of normal supernovae, competing with this standard engine. In this paper, these competing engines, and the constraints placed by compact remnant observations on these engines, are reviewed. We find that, with these constraints, such rotationally-powered engines can explain less than 1% of the current supernova remnants. In addition, we find that the remnant mass distribution can be used to constrain properties of the convection-enhanced neutrino-driven engine, helping astronomers understand the nature of convection in this engine.
INTEGRAL contributions to magnetars and multimessenger astrophysics
Authors: Mereghetti Sandro
Published: February 2023

The INTEGRAL satellite, in orbit since October 2002, has significantly contributed to the study of magnetars and, thanks to its unique capabilities for the study of transient gamma-ray phenomena, it is now playing an important role in multimessenger astrophysics. The most recent results include the discovery of a peculiar burst from SGR J1935+2154, which gave the first observational evidence for the connection between magnetars and fast radio bursts, and extensive searches for bursting activity in peculiar sources, such as the repeating FRB 20200120E in M81 and the ultra-long period magnetar candidate GLEAMX J162759.5–523504.3.
The Off-Axis Afterglow of GW170817: Flux Prediction at Very High Energies
Authors: Pellouin Clément , Daigne Frédéric
Published: February 2023

The binary neutron star merger gravitational-wave event GW170817 and observations of the subsequent electromagnetic signals at different wavelengths have helped better understand the outflows that follow these mergers. In particular, the off-axis afterglow of the jetted ejecta has allowed to probe the lateral structure of such jets, especially thanks to VLBI imagery of the source. In this work, we model this afterglow including a decelerating jet with lateral structure, while synchrotron emission and synchrotron self-Compton scatterings power the jet radiation. In particular, we extend our analysis to very high energies and predict the light curve in the energy range of H.E.S.S. and the CTA. We finally discuss how future detections of afterglows by these observatories can help break the degeneracies in some key physical parameter measurements, and allow to probe efficiently a sub-population of fast-merging binaries.
Probing Magnetars Using Spectral Lines with Future Telescopes
Authors: Kırmızıbayrak Demet , Heyl Jeremy
Published: February 2023

We present our findings on magnetar spectral line analysis in the context of upcoming high resolution, high effective area, high throughput X-ray telescopes for two cases: persistent magnetar emission and magnetar bursts. For magnetars in quiescence, we present our preliminary work on modelling for phase-resolved emission. Our results reveal the necessity of constraining line depth and width concurrently with line energy to conclusively determine hotspot emission and corresponding magnetic field geometry. We then present the results of our simulations using effective area and response of various current and upcoming X-ray telescopes for magnetar spectral line detection and expand on the exciting opportunities upcoming telescopes provide to probe quiescent and burst emission region geometry and propagation in the extreme magnetic field of a magnetar.
The simulations of early kilonova emission from neutron star mergers
Authors: Banerjee Smaranika , Tanaka Masaomi , Kawaguchi Kyohei , Kato Daiji , Gaigalas Gediminas
Published: February 2023

In the neutron-star mergers, the radioactive decay of freshly synthesized heavy elements produces emissions in the ultraviolet-optical-infrared range, producing a transient called kilonova. The observational properties of the kilonova depend on the bound-bound opacity of the heavy elements, which was largely unavailable for the conditionssuitable at an early time (t < day). In this article, I share some of our recent progress on modeling the early kilonova light curve, focusing on the atomic opacity calculation.
X-ray Polarization at the Crossroads
Authors: Heyl Jeremy , Caiazzo Ilaria , González-Caniulef Denis
Published: February 2023

We argue that measurements of X-ray polarization using the recently launched Imaging X-ray Polarimetry Explorer will answer many open questions about magnetars in particular the physical state of their surfaces, whether vacuum birefringence exists, and the nature of the hard X-ray emission from these objects. We outline the capabilities of the instrument, specific models and the results of simulations for the magnetar 4U 0142+61.
Identification of a Local Sample of Gamma-Ray Bursts Consistent with a Magnetar Giant Flare Origin
Authors: Negro Michela , Burns Eric
Published: February 2023

Triggered by the MGF detected from the Sculptor galaxy on April 2020, the study described in this proceeding reports the unambiguous identification of a distinct population of 4 local (< 5 Mpc) short GRBs, whose rise time and isotropic energy release are independently inconsistent with the larger short GRB population at >99.9% confidence. These properties, the host galaxies, and non-detection in gravitational waves all point to an extragalactic MGF origin. The inferred volumetric rates for events above 4 × 1044 erg of
$$R{\rm{ = }}3.8_{ - 3.1}^{ + 4.0} \times {10^5}Gp{c^{ - 3}}y{r^{ - 1}}$$
. These rates imply that some magnetars produce multiple MGFs, providing a source of repeating GRBs. The rates and host galaxies favor common core-collapse supernova as key progenitors of magnetars.
Rates and environments of neutron star binaries
Authors: Dvorkin Irina
Published: February 2023

Recent observations of the various phases in the lives of neutron star binaries across different messengers have led to major discoveries and raised new exciting questions about the environments of these systems. We will overview the current observational constraints on the formation rates and environments of neutron star binaries, as well as theoretical predictions that will be confronted with future observations.
Search for gravitational-wave transients associated with magnetar bursts during the third Advanced LIGO and Advanced Virgo observing run
Authors: Merfeld Kara
Published: February 2023

Magnetars are neutron stars with exceptionally strong dipole magnetic fields which are observed to display a range of x-ray flaring behavior, but the flaring mechanism is not well understood. The third observing run of Advanced LIGO and Virgo extended from April 1, 2019 to March 27, 2020, and contained x-ray flares from known magnetar SGR 1935+2154, as well as the newly-discovered magnetar, Swift J1818-1607. We search for gravitational waves coincident with these magnetar flares with minimally modeled, coherent searches which specifically target both short-duration gravitational waves produced by excited f-modes in the magnetar’s core, as well as long-duration gravitational waves motivated by the Quasi-Periodic Oscillations observed in the tails of giant flares. In this talk, we report on the methods and sensitivity estimates of there searches, and the astrophysical implications.
Fast Radio Bursts: neutron stars, magnetars or something else?
Authors: Kramer Michael
Published: February 2023

Fast Radio Bursts (FRBs) are millisecond-long bursts of radio emission of extragalactic origin. The nature or FRBs is still unknown. Whether all FRBs are representatives of the same source population, or whether multiple underlying populations exist, is also unknown. One class that stands out is that of the “repeaters”, i.e. FRBs from which multiple bursts have been detected. In these cases, appropriate models should be non-cataclysmic but yet being able to create powerful coherent radio emission. Magnetars are among those source types that are considered as possible explanation for (repeating) FRBs. This review will summarise the basic properties of FRBs and those of magnetars to provide a critical assessment of the possible physical connection between these classes of sources. We conclude that while magnetars may indeed be related to the FRB phenomenon, it is unlikely that they explain all FRBs, i.e. at least two classes of FRBs exist.
Alcohol use as a predictor of the course of major depressive disorder: a prospective population-based study
Authors: Schouten Maria J. E. , ten Have Margreet , Tuithof Marlous , de Graaf Ron , Dekker Jack J. M. , Goudriaan Anna E. , Blankers Matthijs
Published: February 2023

Aims

There are indications that problematic alcohol use may negatively impact the course of major depressive disorder (MDD). However, most studies on alcohol use and adverse MDD outcomes are conducted amongst MDD populations with (severe) alcohol use disorder in psychiatric treatment settings. Therefore, it remains unclear whether these results can be generalised to the general population. In light of this, we examined the longitudinal relationship between alcohol use and MDD persistence after a 3-year follow-up amongst people with MDD from the general population.

Methods

Data were derived from the Netherlands Mental Health Survey and Incidence Study-2 (NEMESIS-2), a psychiatric epidemiological prospective study comprising four waves amongst the adult Dutch general population (n = 6.646). The study sample (n = 642) consisted of those with 12-month MDD who participated at the follow-up wave. The outcome was 12-month MDD persistence after the 3-year follow-up, which was assessed via the Composite International Diagnostic Interview version 3.0. Weekly alcohol consumption was operationalised as non-drinking (0 drinks), low-risk drinking (⩽7 drinks; reference), at-risk drinking (women 8–13 drinks, men 8–20 drinks) and high-risk drinking (women ⩾14, men ⩾21 drinks). We performed univariate and multiple logistic regression analyses, which were adjusted for various socio-demographic and health-related factors.

Results

The majority (67.4%) of the MDD sample were female, while the mean age was 47.1 years. Amongst these, 23.8% were non-drinkers, 52.0% were low-risk drinkers and 14.3% and 9.4% were at-risk and high-risk drinkers, respectively. Around one-quarter of the sample (23.6%) met the criteria for a persistent MDD after 3-year follow-up. No statistically significant association was found between alcohol use and MDD persistence, either for the crude model or the adjusted models. In comparison to low-risk drinking, the full adjusted model showed no statistically significant associations between MDD persistence and non-drinking (odds ratio (OR) = 1.15, p = 0.620), at-risk drinking (OR = 1.25, p = 0.423), or high-risk drinking (OR = 0.74, p = 0.501).

Conclusions

Contrary to our expectations, our findings showed that alcohol use was not a predictor of MDD persistence after 3-year follow-up amongst people with MDD from the general population.
Metabolic cross-resistance to florpyrauxifen-benzyl in barnyardgrass (Echinochloa crus-galli) evolved before the commercialization of Rinskor™
Authors: Takano Hudson K. , Greenwalt Scott , Ouse Dave , Zielinski Moriah , Schmitzer Paul
Published: February 2023

Herbicide options for selective control of monocot weeds in rice (Oryza sativa L.) have historically been limited to a few modes of action such as inhibitors of acetolactate synthase (e.g., penoxsulam, imazamox), photosystem II (e.g., propanil), and acetyl-CoA carboxylase (e.g., cyhalofop). Florpyrauxifen-benzyl (Rinskor™) is a synthetic auxin molecule introduced to the U.S. rice herbicide market in 2018, providing broad-spectrum weed control (monocots and dicots), including hard-to-control species such as barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.], along with postemergence rice selectivity at very low use rates. Within the year of commercialization, field agronomists and academics identified E. crus-galli escapes in some areas where florpyrauxifen-benzyl had been sprayed. Further evaluation under controlled environments confirmed that those plants were able to survive florpyrauxifen-benzyl application at the label rate. Here, we identify the mechanism of resistance to florpyrauxifen-benzyl and penoxsulam in two E. crus-galli populations from Arkansas (AR-27) and Missouri (MO-18). Using high-resolution mass spectrometry, we compared the two resistant biotypes with known susceptible plants regarding their ability to metabolize florpyrauxifen-benzyl, florpyrauxifen-acid, and penoxsulam in planta. We discovered that the resistant plants share a common resistance mechanism to florpyrauxifen-benzyl and penoxsulam, involving hydrolysis of a methoxy group (likely mediated by a cytochrome P450 monooxygenase) followed by glucose conjugation. Given that penoxsulam has been widely used in rice fields for the past decade, these data suggest that some populations of E. crus-galli may have evolved resistance before the commercialization of florpyrauxifen-benzyl.
A Balanced Atmospheric Ensemble Forcing for Sea Ice Modeling in Southern Ocean
Authors: Luo Hao , Yang Qinghua , Mazloff Matthew , Chen Dake
Published: February 2023

To deal with the great challenges in simulating the highly non‐linear physics of Antarctic sea ice, a multivariate balanced atmospheric ensemble forcing is developed based on the high‐resolution component of ERA5, which considers the relationship between different variables and adjacent times. To validate the performance of this new forcing, experiments were conducted from 1 January 2016 to 28 February 2017. Compared to simulations forced with the ensemble component of ERA5, a more reasonable ensemble of simulations is produced by the atmospheric ensemble forcing developed in this study, which suppresses the sea‐ice concentration (SIC) simulation errors and produces a better estimation of SIC simulation uncertainties. Further sea‐ice thickness budget analysis reveals that this impact of atmospheric ensemble forcing on sea ice simulation is due to a modulation of atmosphere‐ocean and sea ice‐ocean thermodynamic processes. These results lay the foundation for further improvements in Antarctic sea ice data assimilation and probabilistic prediction.
Effects of Heavy Ions on the Second‐Harmonic Generation of EMIC Waves in the Inner Magnetosphere
Authors: Xue Zuxiang , Yuan Zhigang , Yu Xiongdong , Deng Dan , Huang Zheng
Published: February 2023

Recent studies have reported electromagnetic ion cyclotron (EMIC) waves with nonlinear second harmonics (SHs) whose frequency and wave number are twice those of the fundamental waves (FWs). In this paper, we provide detailed analyses of the cross‐band SH generation of EMIC waves in a multi‐ion plasma. In the presence of heavy ions, EMIC wave modes can be divided into distinct wave bands according to heavy ion gyrofrequencies, and the SH can exist in a wave band different from that of the FW. The SH can be generated around or even exactly on the normal modes of the plasma system, leading to a more efficient energy transfer than that in H+‐only plasmas. The detailed parametric analysis also manifests the vital role of heavy ions in promoting SH's amplitude. Further, multiple 1‐D hybrid simulations are performed to investigate the cross‐band SH generation and validate the theoretical results. The SHs are well reproduced in simulations and the quantitative relation of frequency and wave number between the FW and the SH is well satisfied. The variations of the amplitude ratios (the SH to the FW) with fundamental frequencies are well consistent with those obtained from theoretical calculations in magnitude. Therefore, our results demonstrate the significant roles of heavy ions in the SH generation.
Force‐Free Current Sheets in the Jovian Magnetodisk: The Key Role of Electron Field‐Aligned Anisotropy
Authors: Artemyev A. V. , Ma Q. , Ebert R. W. , Zhang X.‐J. , Allegrini F.
Published: February 2023

Current sheets are an essential element of the planetary magnetotails, where strong plasma currents self‐consistently support magnetic field gradients. The current sheet configuration is determined by plasma populations that contribute to the current density. The most commonly investigated configuration is supported by diamagnetic cross‐field currents of hot ions, typical for the magnetospheres of magnetized planets. In this study, we examine a new type of the current sheet configuration supported by field‐aligned currents from electron streams in the Jovian magnetodisk. Such bi‐directional streams increase the electron thermal anisotropy close to the fire‐hose instability threshold and lead to strong magnetic field shear. The current sheet configuration supported by electron streams is nearly force‐free, with |B| ≈ const across the sheet. Using Juno plasma and magnetic field measurements, we investigate the internal structure of such current sheets and discuss possible mechanisms for their formation.
Water Mass Variations in the Maluku Channel of the Indonesian Seas During the Winter of 2018–2019
Authors: Wang Zheng , Yin Xueli , Li Xiang , Li Yao , Li Rui , Yang Ya , Mamuaja Jane M. , Pangalila Fransisco , Kalangi Patrice , Gerung Grevo , Purwandana Adi , Wardana Adhitya Kusuma , Surinati Dewi , Ismail Mochamad Furqon Azis , Dirhamsyah Dirham , Arifin Zainal , Yuan Dongliang
Published: February 2023

An autonomous underwater glider was deployed in the Maluku Channel during 1 December 2018 through 21 February 2019 to measure temperature and salinity profiles of the upper ∼1,000 m continuously. The measurements suggest intraseasonal variations of the Pacific water mass intrusions into the Maluku Channel, which has not been reported before. The North Pacific Tropical and Intermediate Waters are found to intrude episodically into the eastern Maluku Channel at the depths of 130–180 m and 250–300 m, respectively, during January through February of 2019, each lasting for a few days to a week. The Antarctic Intermediate Water was present in the middle and western Maluku Channel between 600 and 800 m with intraseasonal variability. The South Pacific Subsurface Water was only found to intrude into the eastern Maluku Channel between 250 and 300 m during the first two transections in early December of 2018. Concurrent meter data from three moorings in the Maluku and Talaud‐Halmahera Channels are used to verify the water mass movement. The subsurface and intermediate water mass variations here are found to be closely related to the swing of the Mindanao Current near the Maluku Channel. These intraseasonal water mass intrusions are important aspects of water exchange between the western Pacific Ocean and the Indonesian seas.
Molecular and functional imaging in cancer-targeted therapy: current applications and future directions
Authors: Bai Jing-Wen , Qiu Si-Qi , Zhang Guo-Jun
Published: February 2023

Targeted anticancer drugs block cancer cell growth by interfering with specific signaling pathways vital to carcinogenesis and tumor growth rather than harming all rapidly dividing cells as in cytotoxic chemotherapy. The Response Evaluation Criteria in Solid Tumor (RECIST) system has been used to assess tumor response to therapy via changes in the size of target lesions as measured by calipers, conventional anatomically based imaging modalities such as computed tomography (CT), and magnetic resonance imaging (MRI), and other imaging methods. However, RECIST is sometimes inaccurate in assessing the efficacy of targeted therapy drugs because of the poor correlation between tumor size and treatment-induced tumor necrosis or shrinkage. This approach might also result in delayed identification of response when the therapy does confer a reduction in tumor size. Innovative molecular imaging techniques have rapidly gained importance in the dawning era of targeted therapy as they can visualize, characterize, and quantify biological processes at the cellular, subcellular, or even molecular level rather than at the anatomical level. This review summarizes different targeted cell signaling pathways, various molecular imaging techniques, and developed probes. Moreover, the application of molecular imaging for evaluating treatment response and related clinical outcome is also systematically outlined. In the future, more attention should be paid to promoting the clinical translation of molecular imaging in evaluating the sensitivity to targeted therapy with biocompatible probes. In particular, multimodal imaging technologies incorporating advanced artificial intelligence should be developed to comprehensively and accurately assess cancer-targeted therapy, in addition to RECIST-based methods.
Cross-tissue correlations of genome-wide DNA methylation in Japanese live human brain and blood, saliva, and buccal epithelial tissues
Authors: Nishitani Shota , Isozaki Makoto , Yao Akiko , Higashino Yoshifumi , Yamauchi Takahiro , Kidoguchi Masamune , Kawajiri Satoshi , Tsunetoshi Kenzo , Neish Hiroyuki , Imoto Hirochika , Arishima Hidetaka , Kodera Toshiaki , Fujisawa Takashi X. , Nomura Sadahiro , Kikuta Kenichiro , Shinozaki Gen , Tomoda Akemi
Published: February 2023

Neuroepigenetics considers genetic sequences and the interplay with environmental influences to elucidate vulnerability risk for various neurological and psychiatric disorders. However, evaluating DNA methylation of brain tissue is challenging owing to the issue of tissue specificity. Consequently, peripheral surrogate tissues were used, resulting in limited progress compared with other epigenetic studies, such as cancer research. Therefore, we developed databases to establish correlations between the brain and peripheral tissues in the same individuals. Four tissues, resected brain tissue, blood, saliva, and buccal mucosa (buccal), were collected from 19 patients (aged 13–73 years) who underwent neurosurgery. Moreover, their genome-wide DNA methylation was assessed using the Infinium HumanMethylationEPIC BeadChip arrays to determine the cross-tissue correlation of each combination. These correlation analyses were conducted with all methylation sites and with variable CpGs, and with when these were adjusted for cellular proportions. For the averaged data for each CpG across individuals, the saliva–brain correlation (r = 0.90) was higher than that for blood–brain (r = 0.87) and buccal–brain (r = 0.88) comparisons. Among individual CpGs, blood had the highest proportion of CpGs correlated to the brain at nominally significant levels (19.0%), followed by saliva (14.4%) and buccal (9.8%). These results were similar to the previous IMAGE-CpG results; however, cross-database correlations of the correlation coefficients revealed a relatively low (brain vs. blood: r = 0.27, saliva: r = 0.18, and buccal: r = 0.24). To the best of our knowledge, this is the fifth study in the literature initiating the development of databases for correlations between the brain and peripheral tissues in the same individuals. We present the first database developed from an Asian population, specifically Japanese samples (AMAZE-CpG), which would contribute to interpreting individual epigenetic study results from various Asian populations.
LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axis
Authors: Ye Deji , Liu Hanshao , Zhao Guojun , Chen Aijun , Jiang Yuhang , Hu Yiming , Liu Dandan , Xie Ningxia , Liang Weifei , Chen Xi , Zhang Haohao , Li Cuifeng , Wang Jingyao , Sun Donglin , Chen Weifeng , Tan Dan , Wang Qi , Wang Hongru , Yu Dianping , Wu Baojin , Wang Mingliang , Cui Shuzhong , Liu Sanhong , Zhang Xiaoren
Published: February 2023

Chronic inflammation promotes the tumorigenesis and cell stemness maintenance of colorectal cancer (CRC). However, the bridge role of long noncoding RNA (lncRNA) in linking chronic inflammation to CRC development and progression needs better understanding. Here, we elucidated a novel function of lncRNA GMDS-AS1 in persistently activated signal transducer and transcription activator 3 (STAT3) and Wnt signaling and CRC tumorigenesis. Interleukin-6 (IL-6) and Wnt3a induced lncRNA GMDS-AS1 expression, which was highly expressed in the CRC tissues and plasma of CRC patients. GMDS-AS1 knockdown impaired the survival, proliferation and stem cell-like phenotype acquisition of CRC cells in vitro and in vivo. We performed RNA sequencing (RNA-seq) and mass spectrometry (MS) to probe target proteins and identify their contributions to the downstream signaling pathways of GMDS-AS1. In CRC cells, GMDS-AS1 physically interacted with the RNA-stabilizing protein HuR, thereby protecting the HuR protein from polyubiquitination- and proteasome-dependent degradation. HuR stabilized STAT3 mRNA and upregulated the levels of basal and phosphorylated STAT3 protein, persistently activating STAT3 signaling. Our research revealed that the lncRNA GMDS-AS1 and its direct target HuR constitutively activate STAT3/Wnt signaling and promote CRC tumorigenesis, the GMDS-AS1-HuR-STAT3/Wnt axis is a therapeutic, diagnostic and prognostic target in CRC.
Scalable synthesis of Cu clusters for remarkable selectivity control of intermediates in consecutive hydrogenation
Authors: Yao Dawei , Wang Yue , Li Ying , Li Antai , Zhen Ziheng , Lv Jing , Sun Fanfei , Yang Ruoou , Luo Jun , Jiang Zheng , Wang Yong , Ma Xinbin
Published: February 2023

Subnanometric Cu clusters that contain only a small number of atoms exhibit unique and, often, unexpected catalytic behaviors compared with Cu nanoparticles and single atoms. However, due to the high mobility of Cu species, scalable synthesis of stable Cu clusters is still a major challenge. Herein, we report a facile and practical approach for scalable synthesis of stable supported Cu cluster catalysts. This method involves the atomic diffusion of Cu from the supported Cu nanoparticles to CeO2 at a low temperature of 200 °C to form stable Cu clusters with tailored sizes. Strikingly, these Cu clusters exhibit high yield of intermediate product (95%) in consecutive hydrogenation reactions due to their balanced adsorption of the intermediate product and dissociation of H2. The scalable synthesis strategy reported here makes the stable Cu cluster catalysts one step closer to practical semi-hydrogenation applications.
mcPGK1-dependent mitochondrial import of PGK1 promotes metabolic reprogramming and self-renewal of liver TICs
Authors: Chen Zhenzhen , He Qiankun , Lu Tiankun , Wu Jiayi , Shi Gaoli , He Luyun , Zong Hong , Liu Benyu , Zhu Pingping
Published: February 2023

Liver tumour-initiating cells (TICs) contribute to tumour initiation, metastasis, progression and drug resistance. Metabolic reprogramming is a cancer hallmark and plays vital roles in liver tumorigenesis. However, the role of metabolic reprogramming in TICs remains poorly explored. Here, we identify a mitochondria-encoded circular RNA, termed mcPGK1 (mitochondrial circRNA for translocating phosphoglycerate kinase 1), which is highly expressed in liver TICs. mcPGK1 knockdown impairs liver TIC self-renewal, whereas its overexpression drives liver TIC self-renewal. Mechanistically, mcPGK1 regulates metabolic reprogramming by inhibiting mitochondrial oxidative phosphorylation (OXPHOS) and promoting glycolysis. This alters the intracellular levels of α-ketoglutarate and lactate, which are modulators in Wnt/β-catenin activation and liver TIC self-renewal. In addition, mcPGK1 promotes PGK1 mitochondrial import via TOM40 interactions, reprogramming metabolism from oxidative phosphorylation to glycolysis through PGK1-PDK1-PDH axis. Our work suggests that mitochondria-encoded circRNAs represent an additional regulatory layer controlling mitochondrial function, metabolic reprogramming and liver TIC self-renewal.
Allometry reveals trade-offs between Bergmann’s and Allen’s rules, and different avian adaptive strategies for thermoregulation
Authors: Frӧhlich Arkadiusz , Kotowska Dorota , Martyka Rafał , Symonds Matthew R. E.
Published: February 2023

Animals tend to decrease in body size (Bergmann’s rule) and elongate appendages (Allen’s rule) in warm climates. However, it is unknown whether these patterns depend on each other or constitute independent responses to the thermal environment. Here, based on a global phylogenetic comparative analysis across 99.7% of the world’s bird species, we show that the way in which the relative length of unfeathered appendages co-varies with temperature depends on body size and vice versa. First, the larger the body, the greater the increase in beak length with temperature. Second, the temperature-based increase in tarsus length is apparent only in larger birds, whereas in smaller birds, tarsus length decreases with temperature. Third, body size and the length of beak and tarsus interact with each other to predict the species’ environmental temperature. These findings suggest that the animals’ body size and shape are products of an evolutionary compromise that reflects distinct alternative thermoregulatory adaptations.
Scalable and switchable CO2-responsive membranes with high wettability for separation of various oil/water systems
Authors: Wang Yangyang , Yang Shaokang , Zhang Jingwei , Chen Zhuo , Zhu Bo , Li Jian , Liang Shijing , Bai Yunxiang , Xu Jianhong , Rao Dewei , Dong Liangliang , Zhang Chunfang , Yang Xiaowei
Published: February 2023

Smart membranes with responsive wettability show promise for controllably separating oil/water mixtures, including immiscible oil-water mixtures and surfactant-stabilized oil/water emulsions. However, the membranes are challenged by unsatisfactory external stimuli, inadequate wettability responsiveness, difficulty in scalability and poor self-cleaning performance. Here, we develop a capillary force-driven confinement self-assembling strategy to construct a scalable and stable CO2-responsive membrane for the smart separation of various oil/water systems. In this process, the CO2-responsive copolymer can homogeneously adhere to the membrane surface by manipulating the capillary force, generating a membrane with a large area up to 3600 cm2 and excellent switching wettability between high hydrophobicity/underwater superoleophilicity and superhydrophilicity/underwater superoleophobicity under CO2/N2 stimulation. The membrane can be applied to various oil/water systems, including immiscible mixtures, surfactant-stabilized emulsions, multiphase emulsions and pollutant-containing emulsions, demonstrating high separation efficiency (>99.9%), recyclability, and self-cleaning performance. Due to robust separation properties coupled with the excellent scalability, the membrane shows great implications for smart liquid separation.
Kagome qubit ice
Authors: Lopez-Bezanilla Alejandro , Raymond Jack , Boothby Kelly , Carrasquilla Juan , Nisoli Cristiano , King Andrew D.
Published: February 2023

Topological phases of spin liquids with constrained disorder can host a kinetics of fractionalized excitations. However, spin-liquid phases with distinct kinetic regimes have proven difficult to observe experimentally. Here we present a realization of kagome spin ice in the superconducting qubits of a quantum annealer, and use it to demonstrate a field-induced kinetic crossover between spin-liquid phases. Employing fine control over local magnetic fields, we show evidence of both the Ice-I phase and an unconventional field-induced Ice-II phase. In the latter, a charge-ordered yet spin-disordered topological phase, the kinetics proceeds via pair creation and annihilation of strongly correlated, charge conserving, fractionalized excitations. As these kinetic regimes have resisted characterization in other artificial spin ice realizations, our results demonstrate the utility of quantum-driven kinetics in advancing the study of topological phases of spin liquids.
Broadband physical layer cognitive radio with an integrated photonic processor for blind source separation
Authors: Zhang Weipeng , Tait Alexander , Huang Chaoran , Ferreira de Lima Thomas , Bilodeau Simon , Blow Eric C. , Jha Aashu , Shastri Bhavin J. , Prucnal Paul
Published: February 2023

The expansion of telecommunications incurs increasingly severe crosstalk and interference, and a physical layer cognitive method, called blind source separation (BSS), can effectively address these issues. BSS requires minimal prior knowledge to recover signals from their mixtures, agnostic to the carrier frequency, signal format, and channel conditions. However, previous electronic implementations did not fulfil this versatility due to the inherently narrow bandwidth of radio-frequency (RF) components, the high energy consumption of digital signal processors (DSP), and their shared weaknesses of low scalability. Here, we report a photonic BSS approach that inherits the advantages of optical devices and fully fulfils its “blindness” aspect. Using a microring weight bank integrated on a photonic chip, we demonstrate energy-efficient, wavelength-division multiplexing (WDM) scalable BSS across 19.2 GHz processing bandwidth. Our system also has a high (9-bit) resolution for signal demixing thanks to a recently developed dithering control method, resulting in higher signal-to-interference ratios (SIR) even for ill-conditioned mixtures.
Increased activity of lacrimal gland mast cells are associated with corneal epitheliopathy in aged mice
Authors: Elbasiony Elsayed , Cho WonKyung J. , Singh Aastha , Mittal Sharad K. , Zoukhri Driss , Chauhan Sunil K.
Published: February 2023

The lacrimal gland undergoes significant structural and functional deterioration with aging. Marked with increased inflammation and fibrosis, the aged lacrimal gland is unable to perform its protective function. As a result, the ocular surface becomes highly susceptible to various ocular surface pathologies, including corneal epitheliopathy. We and others have previously shown that mast cells mediate tissue inflammation by recruiting other immune cells. However, despite their well-known characteristics of secreting various inflammatory mediators, whether mast cells contribute to the immune cell aggregation and activation, and acinar dystrophy of the aged lacrimal gland has not been investigated. Here, we demonstrate the role of mast cells in age-related lacrimal gland pathophysiology using mast cell-deficient (cKitw-sh) mice. Our data demonstrated a significant increase in mast cell frequencies and immune cell infiltration in the lacrimal gland of aged mice. Interestingly, mast cell deficiency resulted in a substantial reduction in inflammation and preservation of lacrimal gland structure, suggesting that mast cells mediate the aging process of the lacrimal gland.
Ubiquitin-like conjugation by bacterial cGAS enhances anti-phage defence
Authors: Jenson Justin M. , Li Tuo , Du Fenghe , Ea Chee-Kwee , Chen Zhijian J.
Published: February 2023

cGAS is an evolutionarily conserved enzyme that has a pivotal role in immune defence against infection1–3. In vertebrate animals, cGAS is activated by DNA to produce cyclic GMP–AMP (cGAMP)4,5, which leads to the expression of antimicrobial genes6,7. In bacteria, cyclic dinucleotide (CDN)-based anti-phage signalling systems (CBASS) have been discovered8–11. These systems are composed of cGAS-like enzymes and various effector proteins that kill bacteria on phage infection, thereby stopping phage spread. Of the CBASS systems reported, approximately 39% contain Cap2 and Cap3, which encode proteins with homology to ubiquitin conjugating (E1/E2) and deconjugating enzymes, respectively8,12. Although these proteins are required to prevent infection of some bacteriophages8, the mechanism by which the enzymatic activities exert an anti-phage effect is unknown. Here we show that Cap2 forms a thioester bond with the C-terminal glycine of cGAS and promotes conjugation of cGAS to target proteins in a process that resembles ubiquitin conjugation. The covalent conjugation of cGAS increases the production of cGAMP. Using a genetic screen, we found that the phage protein Vs.4 antagonized cGAS signalling by binding tightly to cGAMP (dissociation constant of approximately 30 nM) and sequestering it. A crystal structure of Vs.4 bound to cGAMP showed that Vs.4 formed a hexamer that was bound to three molecules of cGAMP. These results reveal a ubiquitin-like conjugation mechanism that regulates cGAS activity in bacteria and illustrates an arms race between bacteria and viruses through controlling CDN levels.
Deconvolution of clinical variance in CAR-T cell pharmacology and response
Authors: Kirouac Daniel C. , Zmurchok Cole , Deyati Avisek , Sicherman Jordan , Bond Chris , Zandstra Peter W.
Published: February 2023

Chimeric antigen receptor T cell (CAR-T) expansion and persistence vary widely among patients and predict both efficacy and toxicity. However, the mechanisms underlying clinical outcomes and patient variability are poorly defined. In this study, we developed a mathematical description of T cell responses wherein transitions among memory, effector and exhausted T cell states are coordinately regulated by tumor antigen engagement. The model is trained using clinical data from CAR-T products in different hematological malignancies and identifies cell-intrinsic differences in the turnover rate of memory cells and cytotoxic potency of effectors as the primary determinants of clinical response. Using a machine learning workflow, we demonstrate that product-intrinsic differences can accurately predict patient outcomes based on pre-infusion transcriptomes, and additional pharmacological variance arises from cellular interactions with patient tumors. We found that transcriptional signatures outperform T cell immunophenotyping as predictive of clinical response for two CD19-targeted CAR-T products in three indications, enabling a new phase of predictive CAR-T product development.