bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Accelerated High Temperature on Oxidation and Polymerization of Biodiesel from Vegetable Oils
Authors: Kim Jae-Kon , Jeon Cheol-Hwan , Lee Hyung Won , Park Young-Kwon , Min Kyong-il , Hwang In-ha , Kim Young-Min
Published: December 2018

Oxidation of biodiesel (BD) obtained from the decomposition of biomass can damage the fuel injection and engine parts during its use as a fuel. The excess heating of vegetable oils can also cause polymerization of the biodiesel. The extent of BD oxidation depends on its fatty acid composition. In this study, an accelerated oxidation test of BDs at 95 °C was investigated according to ASTM D 2274 by applying a long-term storage test for 16 weeks. The density, viscosity, and total acid number (TAN) of BDs increased because of the accelerated oxidation. Furthermore, the contents of unsaturated fatty acid methyl esters (FAMEs), C18:2 ME, and C18:3 ME in BDs decreased due to the accelerated oxidation. The 1H-nuclear magnetic resonance spectrum of BDs that were obtained from the accelerated high temperature oxidation at 180 °C for 72 h differed from that of fresh BDs. The mass spectrum obtained from the analysis of the model FAME, linoleic acid (C18:2) methyl ester, which was oxidized at high temperature, indicated the formation of dimers and epoxy dimers of linoleic acid (C18:2) methyl ester by a Diels-Alder reaction.
Hydrodynamic Conjunction of Textured Journal Surface—Bearing for Improved Frictional Response during Warm-Up of an Internal Combustion Engine
Authors: Usman Ali , Riaz Sadia , Park Cheol Woo
Published: December 2018

Considerable prime global energy is used in the transport sector. Significant energy is lost to overcome the internal friction of engines in transport vehicles. Journal bearings are crucial tribo-pairs and passive components that cause energy loss. Frictional losses increase extensively during the warm-up period of an engine due to high lubricant viscosity. Recent tribological developments have shown that surface textures can be a potential solution to reduce friction. A numerical investigation is performed to evaluate the effect of surface texture on the frictional and lubrication performance of a journal bearing at varying thermal operating conditions in an internal combustion engine. Temperature variations during engine warm-up are considered with oil rheology to observe texture-based improvements. Surface texture substantially reduces frictional energy loss during engine warm-up. Eight different monograde and multigrade engine oils are considered, and consistency is observed in texture-based improved outcomes.
Optimization of Wave Energy Converter Arrays by an Improved Differential Evolution Algorithm
Authors: Fang Hong-Wei , Feng Yu-Zhu , Li Guo-Ping
Published: December 2018

Since different incident waves will cause the same array to perform differently with respect to the wave energy converter (WEC), the parameters of the incident wave, including the incident angle and the incident wave number, are taken into account for optimizing the wave energy converter array. Then, the differential evolution (DE) algorithm, which has the advantages of simple operation procedures and a strong global search ability, is used to optimize the wave energy converter array. However, the traditional differential evolution algorithm cannot satisfy the convergence precision and speed simultaneously. In order to make the optimization results more accurate, the concept of an adaptive mutation operator is presented to improve the performance of differential evolution algorithm. It gives the improved algorithm a faster convergence and a higher precision ability. The three-float, five-float, and eight-float arrays were optimized, respectively. It can be concluded that the larger the size of the array is, the greater the interaction between the floats is. Hence, a higher efficiency of wave energy extraction for wave energy converter arrays is achieved by the layout optimization of the array of wave energy converters. The results also show that the optimal layout of the array system is inhomogeneously distributed and that the improved DE algorithm used on array optimization is superior to the traditional DE algorithm.
Spatiotemporal Variations of Aboveground Biomass under Different Terrain Conditions
Authors: Shen Aihua , Wu Chaofan , Jiang Bo , Deng Jinsong , Yuan Weigao , Wang Ke , He Shan , Zhu Enyan , Lin Yue , Wu Chuping
Published: December 2018

Biomass is a key biophysical parameter used to estimate carbon storage and forest productivity. Spatially-explicit estimation of biomass provides invaluable information for carbon stock calculation and scientific forest management. Nevertheless, there still exists large uncertainty concerning the relationship between biomass and influential factors. In this study, aboveground biomass (AGB) was estimated using the random forest algorithm based on remote sensing imagery (Landsat) and field data for three regions with different topographic conditions in Zhejiang Province, China. AGB distribution and change combined with stratified terrain classifications were analyzed to investigate the relations between AGB and topography conditions. The results indicated that AGB in three regions increased from 2010 to 2015 and the magnitude of growth varied with elevation, slope, and aspect. In the basin region, slope had a greater influence on AGB, and we attributed this negative AGB-elevation relationship to ecological forest construction. In the mountain area, terrain features, especially elevation, showed significant relations with AGB. Moreover, AGB and its growth showed positive relations with elevation and slope. In the island region, slope also played a relatively more important role in explaining the relationship. These results demonstrate that AGB varies with terrain conditions and its change is a consequence of interactions between the natural environment and anthropogenic behavior, implying that biomass retrieval based on Landsat imagery could provide considerable important information related to regional heterogeneity investigations.
Spoil Type Influences Soil Genesis and Forest Development on an Appalachian Surface Coal Mine Ten Years after Placement
Authors: Sena Kenton , Agouridis Carmen , Miller Jarrod , Barton Chris
Published: December 2018

Surface mining for coal (or other mineral resources) is a major driver of land-use change around the world and especially in the Appalachian region of the United States. Intentional and well-informed reclamation of surface-mined land is critical for the restoration of healthy ecosystems on these disturbed sites. In Appalachia, the pre-mining land cover is predominately mixed hardwood forest, with rich species diversity. In recent years, Appalachian mine reforestation has become an issue of concern, prompting the development of the Forestry Reclamation Approach, a series of mine reforestation recommendations. One of these recommendations is to use the best available soil substitute; however, the characteristics of the “best” soil substitute have been an issue. This study was initiated to compare the suitability of several types of mine spoil common in the Appalachian region: brown sandstone (Brown), gray sandstone (Gray), mixed spoils (Mixed), and shale (Shale). Experimental plots were established in 2007 with each spoil type replicated three times. These plots were planted with a mix of native hardwood species. Ten years after plot construction and planting, tree growth and canopy cover were highest in Brown, followed by Shale, Mixed, and Gray. Soil conditions (particularly pH) in Brown and Shale were more favorable for native tree growth than Mixed or Gray, largely explaining these differences in tree growth and canopy cover. However, soil chemistry did not clearly explain differences in tree growth between Brown and Shale. These differences were more likely related to differences in near-surface soil temperature, which is related to soil color and available shade.
Calcium and Potassium Imbalance Favours Leaf Blight and Defoliation Caused by Calonectria pteridis in Eucalyptus Plants
Authors: Soares Thaissa P. F. , Pozza Edson A. , Pozza Adélia A. A. , Mafia Reginaldo Gonçalves , Ferreira Maria A.
Published: December 2018

The supply of nutrients in balanced proportions leads to greater crop yields and represents an alternative practice for the management of plant diseases. Accordingly, we investigated the effect of the doses of and the nutritional balance between calcium (Ca) and potassium (K) on the severity of leaf spot and defoliation caused by the fungus Calonectria pteridis. Moreover, the effect of the treatments on the growth of interspecific hybrid eucalyptus clone seedlings (Eucalyptus grandis Hill ex Maiden × E. urophylla S.T. Blake), which are highly susceptible to the disease, was evaluated. The 25 treatments comprised combinations of one of five doses of Ca (1.2, 3.0, 6.0, 9.0 and 12.0 mmol L−1) with one of five doses of K (0.8, 2.0, 4.0, 8.0 and 12.0 mmol L−1) and five replicates of each treatment were included in the study. The supply of high concentrations of K favoured C. pteridis infection and resulted in high disease severity, although defoliation was not observed. However, the supply of both nutrients in excess (12.0 mmol L−1 Ca × 9.0 mmol L−1 K) resulted in a higher disease severity and an increased defoliation percentage (82 and 64%, respectively). Defoliation not associated with Calonectria leaf blight disease was observed with the imbalanced treatments, that is, the treatments combining a low concentration of one nutrient and an excess concentration of the other nutrient. The supply of K at a level near the standard dose (6 mmol L−1) and of Ca at a dose above 4 mmol L−1 (standard dose) ensured high mean values for the morphological variables root and shoot biomass, plant height and chlorophyll a and b contents. These treatments also resulted in low disease severity and defoliation percentages, indicating that a balanced supply of Ca and K ensures reductions in disease severity and defoliation and contributes to higher growth.
Acoustic Streaming and Its Applications
Authors: Wu Junru
Published: December 2018

Broadly speaking, acoustic streaming is generated by a nonlinear acoustic wave with a finite amplitude propagating in a viscid fluid. The fluid volume elements of molecules, d V , are forced to oscillate at the same frequency as the incident acoustic wave. Due to the nature of the nonlinearity of the acoustic wave, the second-order effect of the wave propagation produces a time-independent flow velocity (DC flow) in addition to a regular oscillatory motion (AC motion). Consequently, the fluid moves in a certain direction, which depends on the geometry of the system and its boundary conditions, as well as the parameters of the incident acoustic wave. The small scale acoustic streaming in a fluid is called “microstreaming”. When it is associated with acoustic cavitation, which refers to activities of microbubbles in a general sense, it is often called “cavitation microstreaming”. For biomedical applications, microstreaming usually takes place in a boundary layer at proximity of a solid boundary, which could be the membrane of a cell or walls of a container. To satisfy the non-slip boundary condition, the flow motion at a solid boundary should be zero. The magnitude of the DC acoustic streaming velocity, as well as the oscillatory flow velocity near the boundary, drop drastically; consequently, the acoustic streaming velocity generates a DC velocity gradient and the oscillatory flow velocity gradient produces an AC velocity gradient; they both will produce shear stress. The former is a DC shear stress and the latter is AC shear stress. It was observed the DC shear stress plays the dominant role, which may enhance the permeability of molecules passing through the cell membrane. This phenomenon is called “sonoporation”. Sonoporation has shown a great potential for the targeted delivery of DNA, drugs, and macromolecules into a cell. Acoustic streaming has also been used in fluid mixing, boundary cooling, and many other applications. The goal of this work is to give a brief review of the basic mathematical theory for acoustic microstreaming related to the aforementioned applications. The emphasis will be on its applications in biotechnology.
MafA Expression Preserves Immune Homeostasis in Human and Mouse Islets
Authors: Singh Tania , Sarmiento Luis , Luan Cheng , Prasad Rashmi B. , Johansson Jenny , Cataldo Luis R. , Renström Erik , Soneji Shamit , Cilio Corrado , Artner Isabella
Published: December 2018

Type 1 (T1D) and type 2 (T2D) diabetes are triggered by a combination of environmental and/or genetic factors. Maf transcription factors regulate pancreatic beta (β)-cell function, and have also been implicated in the regulation of immunomodulatory cytokines like interferon-β (IFNβ1). In this study, we assessed MAFA and MAFB co-expression with pro-inflammatory cytokine signaling genes in RNA-seq data from human pancreatic islets. Interestingly, MAFA expression was strongly negatively correlated with cytokine-induced signaling (such as IFNAR1, DDX58) and T1D susceptibility genes (IFIH1), whereas correlation of these genes with MAFB was weaker. In order to evaluate if the loss of MafA altered the immune status of islets, MafA deficient mouse islets (MafA−/−) were assessed for inherent anti-viral response and susceptibility to enterovirus infection. MafA deficient mouse islets had elevated basal levels of Ifnβ1, Rig1 (DDX58 in humans), and Mda5 (IFIH1) which resulted in reduced virus propagation in response to coxsackievirus B3 (CVB3) infection. Moreover, an acute knockdown of MafA in β-cell lines also enhanced Rig1 and Mda5 protein levels. Our results suggest that precise regulation of MAFA levels is critical for islet cell-specific cytokine production, which is a critical parameter for the inflammatory status of pancreatic islets.
The New Moon: Major Advances in Lunar Science Enabled by Compositional Remote Sensing from Recent Missions
Authors: Dhingra Deepak
Published: December 2018

Volatile-bearing lunar surface and interior, giant magmatic-intrusion-laden near and far side, globally distributed layer of purest anorthosite (PAN) and discovery of Mg-Spinel anorthosite, a new rock type, represent just a sample of the brand new perspectives gained in lunar science in the last decade. An armada of missions sent by multiple nations and sophisticated analyses of the precious lunar samples have led to rapid evolution in the understanding of the Moon, leading to major new findings, including evidence for water in the lunar interior. Fundamental insights have been obtained about impact cratering, the crystallization of the lunar magma ocean and conditions during the origin of the Moon. The implications of this understanding go beyond the Moon and are therefore of key importance in solar system science. These new views of the Moon have challenged the previous understanding in multiple ways and are setting a new paradigm for lunar exploration in the coming decade both for science and resource exploration. Missions from India, China, Japan, South Korea, Russia and several private ventures promise continued exploration of the Moon in the coming years, which will further enrich the understanding of our closest neighbor. The Moon remains a key scientific destination, an active testbed for in-situ resource utilization (ISRU) activities, an outpost to study the universe and a future spaceport for supporting planetary missions.
Point of Care Quantitative Assessment of Muscle Health in Older Individuals: An Investigation of Quantitative Muscle Ultrasound and Electrical Impedance Myography Techniques
Authors: Hobson-Webb Lisa D , Zwelling Paul J , Pifer Ashley N , Killelea Carrie M , Faherty Mallory S , Sell Timothy C , Pastva Amy M
Published: December 2018

Background: Muscle health is recognized for its critical role in the functionality and well-being of older adults. Readily accessible, reliable, and inexpensive methods of measuring muscle health are needed to advance research and clinical care. Methods: In this prospective, blinded study, 27 patients underwent quantitative muscle ultrasound (QMUS), standard electrical impedance myography (sEIM), and handheld electrical impedance myography (hEIM) of the anterior thigh musculature by two independent examiners. Subjects also had dual-energy X-ray absorptiometry (DEXA) scans and standardized tests of physical function and strength. Data were analyzed for intra- and inter-rater reliability, along with correlations with DEXA and physical measures. Results: Measures of intra- and inter-rater reliability were excellent (>0.90) for all QMUS, sEIM, and hEIM parameters except intra-rater reliability of rectus femoris echointensity (0.87–0.89). There were moderate, inverse correlations between QMUS, sEIM, and hEIM parameters and measures of knee extensor strength. Moderate to strong correlations (0.57–0.81) were noted between investigational measures and DEXA-measured fat mass. Conclusions: QMUS, sEIM and hEIM were highly reliable in a controlled, same-day testing protocol. Multiple correlations with measures of strength and body composition were noted for each method. Point-of-care technologies may provide an alternative means of measuring health.
The Behaviour of the Equity Yield and Its Relation with the Bond Yield: The Role of Inflation
Authors: McMillan David G.
Published: December 2018

Understanding the behaviour of the equity yield and its relation to the bond yield is important for portfolio managers and those engaged in modelling the interaction between asset classes. During the mid-1900s, the equity yield—which was previously greater than the bond yield—declined, while the bond yield rose and became higher. Research, seeking to understand this, put forward the view that stock and bond return volatility is key. Evidence from the 2000s suggest that the relative values of the equity and bond yield have flipped again, with the former now greater. Empirical evidence presented here shows that during such periods, the hypothesised relation between the equity yield and the bond yield and the two volatilities also changes, with the signs reversed. Moreover, there is noticeable variation in the coefficient values. This paper argues that the relative equity and bond yield values are, to a large extent, driven by inflation volatility. High inflation volatility persisted during the first half of the twentieth century when the equity yield was higher. This was followed by more benign inflation volatility when the bond yield became higher. Evidence for a long span of US data, and shorter German, Japanese, and UK data, suggests the recent rise in the equity yield is accompanied by an uptick in inflation volatility relative to its recent tranquillity.
Proteome Map of Pea (Pisum sativum L.) Embryos Containing Different Amounts of Residual Chlorophylls
Authors: Mamontova Tatiana , Lukasheva Elena , Mavropolo-Stolyarenko Gregory , Proksch Carsten , Bilova Tatiana , Kim Ahyoung , Babakov Vladimir , Grishina Tatiana , Hoehenwarter Wolfgang , Medvedev Sergei , Smolikova Galina , Frolov Andrej
Published: December 2018

Due to low culturing costs and high seed protein contents, legumes represent the main global source of food protein. Pea (Pisum sativum L.) is one of the major legume crops, impacting both animal feed and human nutrition. Therefore, the quality of pea seeds needs to be ensured in the context of sustainable crop production and nutritional efficiency. Apparently, changes in seed protein patterns might directly affect both of these aspects. Thus, here, we address the pea seed proteome in detail and provide, to the best of our knowledge, the most comprehensive annotation of the functions and intracellular localization of pea seed proteins. To address possible intercultivar differences, we compared seed proteomes of yellow- and green-seeded pea cultivars in a comprehensive case study. The analysis revealed totally 1938 and 1989 nonredundant proteins, respectively. Only 35 and 44 proteins, respectively, could be additionally identified after protamine sulfate precipitation (PSP), potentially indicating the high efficiency of our experimental workflow. Totally 981 protein groups were assigned to 34 functional classes, which were to a large extent differentially represented in yellow and green seeds. Closer analysis of these differences by processing of the data in KEGG and String databases revealed their possible relation to a higher metabolic status and reduced longevity of green seeds.
Neuromuscular Junction Changes in a Mouse Model of Charcot-Marie-Tooth Disease Type 4C
Authors: Cipriani Silvia , Phan Vietxuan , Médard Jean-Jacques , Horvath Rita , Lochmüller Hanns , Chrast Roman , Roos Andreas , Spendiff Sally
Published: December 2018

The neuromuscular junction (NMJ) appears to be a site of pathology in a number of peripheral nerve diseases. Charcot-Marie-Tooth (CMT) 4C is an autosomal recessive, early onset, demyelinating neuropathy. Numerous mutations in the SH3TC2 gene have been shown to underlie the condition often associated with scoliosis, foot deformities, and reduced nerve conduction velocities. Mice with exon 1 of the Sh3tc2 gene knocked out demonstrate many of the features seen in patients. To determine if NMJ pathology is contributory to the pathomechanisms of CMT4C we examined NMJs in the gastrocnemius muscle of SH3TC2-deficient mice. In addition, we performed proteomic assessment of the sciatic nerve to identify protein factors contributing to the NMJ alterations and the survival of demyelinated axons. Morphological and gene expression analysis of NMJs revealed a lack of continuity between the pre- and post-synaptic apparatus, increases in post-synaptic fragmentation and dispersal, and an increase in expression of the gamma subunit of the acetylcholine receptor. There were no changes in axonal width or the number of axonal inputs to the NMJ. Proteome investigations of the sciatic nerve revealed altered expression of extracellular matrix proteins important for NMJ integrity. Together these observations suggest that CMT4C pathology includes a compromised NMJ even in the absence of changes to the innervating axon.
Genome-Wide Identification of microRNAs in Response to Salt/Alkali Stress in Medicago truncatula through High-Throughput Sequencing
Authors: Cao Chunyu , Long Ruicai , Zhang Tiejun , Kang Junmei , Wang Zhen , Wang Pingqing , Sun Hao , Yu Jie , Yang Qingchuan
Published: December 2018

Saline-alkaline stress is a universal abiotic stress that adversely affects plant growth and productivity. Saline-alkaline conditions results in plant abnormal transcriptome expression finally manifesting as defective phenotypes. Considerable research has revealed the active role of microRNA in various stress conditions. This study was aimed to identify novel miRNAs and the miRNA expression patterns in the leguminous model plant R108 (Medicago truncatula). The miRNA contained in the total RNA extracted from Medicago truncatula seedlings (72 h) that had been treated with solutions mimicking saline and alkaline soils was subjected to miRNA deep sequencing. The Illumina HiSeq sequencing platform was used to analyze nine small RNA libraries of three treatment groups: distilled water, 20 mM NaCl + Na2SO4 and 5 mM Na2CO3 + NaHCO3. Sequencing revealed that 876 miRNAs including 664 known miRNAs and 212 potential novel miRNAs were present in all the libraries. The miR159 family, miR156 family, miR2086-3p, miR396, miR166, miR319, miR167, miR5213-5p, miR1510 and miR2643 were among the most expressed miRNAs in all libraries. The results of miRNAs expression under treatments were validated by reverse-transcription quantitative PCR (RT-qPCR). Target gene prediction through computational analysis and pathway annotation analysis revealed that the primary pathways affected by stress were related to plant development, including metabolic processes, single-organism processes and response to the stimulus. Our results provide valuable information towards elucidating the molecular mechanisms of salt/alkali tolerance in Medicago truncatula and provide insight into the putative role of miRNAs in plant stress resistance.
Identification of Novel Therapeutic Targets for Pulmonary Arterial Hypertension
Authors: Satoh Kimio , Kikuchi Nobuhiro , Satoh Taijyu , Kurosawa Ryo , Sunamura Shinichiro , Siddique Mohammad Abdul Hai , Omura Junichi , Yaoita Nobuhiro , Shimokawa Hiroaki
Published: December 2018

Pulmonary arterial hypertension (PAH) and chronic thromboembolic pulmonary hypertension (CTEPH) are fatal diseases; however, their pathogenesis still remains to be elucidated. We have recently screened novel pathogenic molecules and have performed drug discovery targeting those molecules. Pulmonary artery smooth muscle cells (PASMCs) in patients with PAH (PAH-PASMCs) have high proliferative properties like cancer cells, which leads to thickening and narrowing of distal pulmonary arteries. Thus, we conducted a comprehensive analysis of PAH-PASMCs and lung tissues to search for novel pathogenic proteins. We validated the pathogenic role of the selected proteins by using tissue-specific knockout mice. To confirm its clinical significance, we used patient-derived blood samples to evaluate the potential as a biomarker for diagnosis and prognosis. Finally, we conducted a high throughput screening and found inhibitors for the pathogenic proteins.
Suppression of IFN-γ Production in Murine Splenocytes by Histamine Receptor Antagonists
Authors: Kamei Miho , Otani Yukie , Hayashi Hidenori , Nakamura Tadaho , Yanai Kazuhiko , Furuta Kazuyuki , Tanaka Satoshi
Published: December 2018

Accumulating evidence suggests that histamine synthesis induced in several types of tumor tissues modulates tumor immunity. We found that a transient histamine synthesis was induced in CD11b+Gr-1+ splenocytes derived from BALB/c mice transplanted with a syngeneic colon carcinoma, CT-26, when they were co-cultured with CT-26 cells. Significant levels of IFN-γ were produced under this co-culture condition. We explored the modulatory roles of histamine on IFN-γ production and found that several histamine receptor antagonists, such as pyrilamine, diphenhydramine, JNJ7777120, and thioperamide, could significantly suppress IFN-γ production. However, suppression of IFN-γ production by these antagonists was also found when splenocytes were derived from the Hdc−/− BALB/c mice. Suppressive effects of these antagonists were found on IFN-γ production induced by concanavalin A or the combination of an anti-CD3 antibody and an anti-CD28 antibody in a histamine-independent manner. Murine splenocytes were found to express H1 and H2 receptors, but not H3 and H4 receptors. IFN-γ production in the Hh1r−/− splenocytes induced by the combination of an anti-CD3 antibody and an anti-CD28 antibody was significantly suppressed by these antagonists. These findings suggest that pyrilamine, diphenhydramine, JNJ7777120, and thioperamide can suppress IFN-γ production in activated splenocytes in a histamine-independent manner.
Initial Relative Position Influencing Self-Assembly of a Black Phosphorus Ribbon on a CNT
Authors: Cao Jing , Wang Yixuan , Shi Jiao , Chai Junrui , Cai Kun
Published: December 2018

It is difficult to obtain a nanotube from phosphorus with a 3sp2 electron configuration by chemical synthesis. However, a physical fabrication approach, such as self-assembly, is worth trying. In an experiment, when using a carbon nanotube (CNT) to trigger self-assembly of a black phosphorus (BP) ribbon, the final configuration of the BP component may be sensitive to the initial relative position of the CNT to the BP ribbon. For instance, using the same CNT with different initial relative positions to the BP ribbon, the BP ribbon may finally become a nanotube, or a scroll, or just wind upon the CNT, or escape from the CNT, etc. In this study, the sensitivity is investigated using molecular dynamics simulations. Numerical results illustrate some essentials for potential fabrication of a BP nanotube from ribbon.
The WRKY Transcription Factor GmWRKY12 Confers Drought and Salt Tolerance in Soybean
Authors: Shi Wen-Yan , Du Yong-Tao , Ma Jian , Min Dong-Hong , Jin Long-Guo , Chen Jun , Chen Ming , Zhou Yong-Bin , Ma You-Zhi , Xu Zhao-Shi , Zhang Xiao-Hong
Published: December 2018

WRKYs are important regulators in plant development and stress responses. However, knowledge of this superfamily in soybean is limited. In this study, we characterized the drought- and salt-induced gene GmWRKY12 based on RNA-Seq and qRT-PCR. GmWRKY12, which is 714 bp in length, encoded 237 amino acids and grouped into WRKY II. The promoter region of GmWRKY12 included ABER4, MYB, MYC, GT-1, W-box and DPBF cis-elements, which possibly participate in abscisic acid (ABA), drought and salt stress responses. GmWRKY12 was minimally expressed in different tissues under normal conditions but highly expressed under drought and salt treatments. As a nucleus protein, GmWRKY12 was responsive to drought, salt, ABA and salicylic acid (SA) stresses. Using a transgenic hairy root assay, we further characterized the roles of GmWRKY12 in abiotic stress tolerance. Compared with control (Williams 82), overexpression of GmWRKY12 enhanced drought and salt tolerance, increased proline (Pro) content and decreased malondialdehyde (MDA) content under drought and salt treatment in transgenic soybean seedlings. These results may provide a basis to understand the functions of GmWRKY12 in abiotic stress responses in soybean.
Molecular Mechanisms and Pathophysiology of Ischemia-Reperfusion Injury
Authors: Panisello-Roselló Arnau , Roselló-Catafau Joan
Published: December 2018

Ischemia-reperfusion injury (IRI) is a major cause of graft loss and dysfunction in clinical transplantation and organ resection. [...]
Combination of a Bioceramic Scaffold and Simvastatin Nanoparticles as a Synthetic Alternative to Autologous Bone Grafting
Authors: Wang Chau-Zen , Wang Yan-Hsiung , Lin Che-Wei , Lee Tien-Ching , Fu Yin-Chih , Ho Mei-Ling , Wang Chih-Kuang
Published: December 2018

The fragile nature of porous bioceramic substitutes cannot match the toughness of bone, which limits the use of these materials in clinical load-bearing applications. Statins can enhance bone healing, but it could show rhabdomyolysis/inflammatory response after overdosing. In this study, the drug-containing bone grafts were developed from poly(lactic acid-co-glycolic acid)-polyethylene glycol (PLGA-PEG) nanoparticles encapsulating simvastatin (SIM) (SIM-PP NPs) loaded within an appropriately mechanical bioceramic scaffold (BC). The combination bone graft provides dual functions of osteoconduction and osteoinduction. The mechanical properties of the bioceramic are enhanced mainly based on the admixture of a combustible reverse-negative thermoresponsive hydrogel (poly(N-isopropylacrylamide base). We showed that SIM-PP NPs can increase the activity of alkaline phosphatase and osteogenic differentiation of bone marrow stem cells. To verify the bone-healing efficacy of this drug-containing bone grafts, a nonunion radial endochondral ossification bone defect rabbit model (N = 3/group) and a nonunion calvarial intramembranous defect Sprague Dawley (SD) rat model (N = 5/group) were used. The results indicated that SIM-PP NPs combined with BC can improve the healing of nonunion bone defects of the radial bone and calvarial bone. Therefore, the BC containing SIM-PP NPs may be appropriate for clinical use as a synthetic alternative to autologous bone grafting that can overcome the problem of determining the clinical dosage of simvastatin drugs to promote bone healing.
TCDD Toxicity Mediated by Epigenetic Mechanisms
Authors: Patrizi Barbara , Siciliani de Cumis Mario
Published: December 2018

Dioxins are highly toxic and persistent halogenated organic pollutants belonging to two families i.e., Polychlorinated Dibenzo-p-Dioxins (PCDDs) and Polychlorinated Dibenzo Furans (PCDFs). They can cause cancer, reproductive and developmental issues, damage to the immune system, and can deeply interfere with the endocrine system. Dioxins toxicity is mediated by the Aryl-hydrocarbon Receptor (AhR) which mediates the cellular metabolic adaptation to these planar aromatic xenobiotics through the classical transcriptional regulation pathway, including AhR binding of ligand in the cytosol, translocation of the receptor to the nucleus, dimerization with the AhR nuclear translocator, and the binding of this heterodimeric transcription factor to dioxin-responsive elements which regulate the expression of genes involved in xenobiotic metabolism. 2,3,7,8-TCDD is the most toxic among dioxins showing the highest affinity toward the AhR receptor. Beside this classical and well-studied pathway, a number of papers are dealing with the role of epigenetic mechanisms in the response to environmental xenobiotics. In this review, we report on the potential role of epigenetic mechanisms in dioxins-induced cellular response by inspecting recent literature and focusing our attention on epigenetic mechanisms induced by the most toxic 2,3,7,8-TCDD.
Task Staggering Peak Scheduling Policy for Cloud Mixed Workloads
Authors: Hu Zhigang , Tao Yong , Zheng Meiguang , Chang Chenglong
Published: December 2018

To address the issue of cloud mixed workloads scheduling which might lead to system load imbalance and efficiency degradation in cloud computing, a novel cloud task staggering peak scheduling policy based on the task types and the resource load status is proposed. First, based on different task characteristics, the task sequences submitted by the user are divided into queues of different types by the fuzzy clustering algorithm. Second, the Performance Counters (PMC) mechanism is introduced to dynamically monitor the load status of resource nodes and respectively sort the resources by the metrics of Central Processing Unit (CPU), memory, and input/output (I/O) load size, so as to reduce the candidate resources. Finally, the task sequences of specific type are scheduled for the corresponding light loaded resources, and the resources usage peak is staggered to achieve load balancing. The experimental results show that the proposed policy can balance loads and improve the system efficiency effectively and reduce the resource usage cost when the system is in the presence of mixed workloads.
Monitoring the Impact of Groundwater Pumping on Infrastructure Using Geographic Information System (GIS) and Persistent Scatterer Interferometry (PSI)
Authors: DePrekel Kirsten , Bouali El Hachemi , Oommen Thomas
Published: December 2018

Transportation infrastructure is critical for the advancement of society. Bridges are vital for an efficient transportation network. Bridges across the world undergo variable deformation/displacement due to the Earth’s dynamic processes. This displacement is caused by ground motion, which occurs from many natural and anthropogenic events. Events causing deformation include temperature fluctuation, subsidence, landslides, earthquakes, water/sea level variation, subsurface resource extraction, etc. Continual deformation may cause bridge failure, putting civilians at risk, if not managed properly. Monitoring bridge displacement, large and small, provides evidence of the state and health of the bridge. Traditionally, bridge monitoring has been executed through on-site surveys. Although this method of bridge monitoring is systematic and successful, it is not the most efficient and cost-effective. Through technological advances, satellite-based Persistent Scatterer Interferometry (PSI) and Geographic Information Systems (GIS) have provided a system for analyzing ground deformation over time. This method is applied to distinguish bridges that are more at risk than others by generating models that display the displacement at various locations along each bridge. A bridge’s health and its potential risk can be estimated upon analysis of measured displacement rates. In return, this process of monitoring bridges can be done at much faster rates; saving time, money and resources. PSI data covering Oxnard, California, revealed both bridge displacement and regional ground displacement. Although each bridge maintained different patterns of displacement, many of the bridges within the Oxnard area displayed an overall downward movement matching regional subsidence trends observed in the area. Patterns in displacement-time series plots provide evidence for two types of deformation mechanisms. Long-term downward movements correlate with the relatively large regional subsidence observed using PSI in Oxnard. Thermal dilation from seasonal temperature changes may cause short-term variabilities unique to each bridge. Overall, it may be said that linking geologic, weather, and groundwater patterns with bridge displacement has shown promise for monitoring transportation infrastructure and more importantly differentiating between regional subsidence and site-specific displacements.
Rhyzobius lophanthae Behavior is Influenced by Cycad Plant Age, Providing Odor Samples in a Y-tube Olfactometer
Authors: Marler Thomas E. , Marler Paris N.
Published: December 2018

The scale predator Rhyzobius lophanthae Blaisdell was introduced to Guam and Rota to control invasive Aulacaspis yasumatsui Takagi armored scale infestations on the native Cycas micronesica K.D. Hill populations. The predator demonstrated a clear preference for A. yasumatsui infesting adult plants, resulting in 100% seedling mortality due to the lack of a biocontrol of the scale on seedlings. A Y-tube olfactometer was employed to determine if scale-infested seedling leaves were less attractive to R. lophanthae than scale-infested mature tree leaves. Five paired combinations of seedling versus mature tree leaves were used. The R. lophanthae adults navigated toward scale-infested and un-infested leaves of adults and seedlings when paired with an empty chamber. However, a clear preference for adult leaves occurred when paired with seedling leaves. The results were unambiguous in charcoal-filtered air, intermediate in unfiltered air from an open laboratory, and most ambiguous when conducted with unfiltered in-situ air. The number of predators that did not make a choice was greatest for in-situ air and least for charcoal-filtered air. These results indicated that the substrate used in olfactometry influenced the results, and interpretations of charcoal-filtered air assays should be made with caution. Volatile chemical cues are involved in R. lophanthae preferring A. yasumatsui located on C. micronesica adults when infested adult and seedling leaves are present.
Public Green Infrastructure Contributes to City Livability: A Systematic Quantitative Review
Authors: Parker Jackie , Simpson Greg D.
Published: December 2018

Consistent with the Land Urbanism and Green Infrastructure theme of this special issue of Land, the primary goal of this review is to provide a plain language overview of recent literature that reports on the psychological, physiological, general well-being, and wider societal benefits that humans receive as a result of experiencing public green infrastructure (PGI) and nature in urbanized landscapes. This enhanced well-being and the wider societal benefits that accrue to urban dwellers as a result of interacting with quality PGI contributes to the concept known as city or urban livability. The quantitative analysis and theoretical synthesis reported in this review can inform decision makers, stakeholders, and other PGI and urban nature (UN) researchers of the benefits that urban populations receive from experiencing quality PGI spaces and UN and the contribution those spaces make to the livability of urban areas. With diminishing opportunities for the acquisition of new public open space to increase PGI and re-establish UN near urban centers, the efficient management and continuous improvement of existing PGI and UN is essential to promote and foster opportunities for human-to-nature contact and the known benefits therein derived. In addition to identifying an increased research interest and publication of articles that report on the contribution of PGI spaces to urban livability over the past decade, the review identifies and reports on the seven focus areas of PGI-livability research and the six attributes of PGI spaces that the current literatures report as contributing to the livability of urbanized landscapes. After providing a quantitative analysis for the reporting of those research areas and PGI attributes and summarizing key findings reported in the literature regarding the contribution that PGI spaces make to urban livability, this review also identifies knowledge gaps in the published literature and puts forward recommendations for further research in this rapidly expanding multidisciplinary field of research and policy development.
Experimental Studies of Lubricant Flow and Friction in Partially Filled Gaps
Authors: Müller Michael , Stahl Lukas , Ostermeyer Georg-Peter
Published: December 2018

Starved lubrication is an important strategy for minimizing the amount of lubricant needed, and also inevitably occurs during idling and fail-safe lubrication. In this regime, however, the flow of the lubricant and the related friction coefficients are yet to be fully understood. This research aims to make fundamental contributions to the understanding of contact mechanics of partially lubricated contacts. Recent experiments with a pin-on-disk tribometer examined the microscopic behavior of partially filled gaps. Using a new experimental setup on a macroscale, new insights into partially filled gaps with rough surfaces were gained. This work presents the systematic analyses of the lubricant flow, friction coefficients, and other variables over a wide range of friction parameters. Distinct friction behaviors were observed, and similar effects occur on both the micro and macroscale. The experimental results show that a typical Stribeck characteristic is visible regarding not only the relative velocity, but also regarding the lubricant filling level in the gap. The fluid exhibits a variety of flow patterns for various velocities and viscosities. The patterns relate to different friction regimes, such as dry friction and mixed lubrication. It is concluded that the filling level is a valid parameter for regulating the transition from dry friction to hydrodynamic lubrication. These findings are quantified regarding the filling level and it is shown that for the identification of the friction regimes the filling level is an independent parameter in addition to the established parameters like speed, viscosity and pressure.
Special Issue: Novel Photoactive Materials
Authors: Diamanti Maria Vittoria
Published: December 2018

Photoactivity represents the ability of a material to activate when interacting with light. It can be declined in many ways, and several functionalities arising from this behavior of materials can be exploited, all leading to positive repercussions on our environment. There are several classes of effects of photoactivity, all of which have been deeply investigated in the last few decades, allowing researchers to develop more and more efficient materials and devices. The special issue “Novel Photoactive Materials” has been proposed as a means to present recent developments in the field; for this reason the articles included touch different aspects of photoactivity, from photocatalysis to photovoltaics to light emitting materials, as highlighted in this editorial.
Recent Developments in the Optimization of the Bulk Heterojunction Morphology of Polymer: Fullerene Solar Cells
Authors: Gaspar Hugo , Figueira Flávio , Pereira Luiz , Mendes Adélio , Viana Júlio C. , Bernardo Gabriel
Published: December 2018

Organic photovoltaic (OPV) devices, made with semiconducting polymers, have recently attained a power conversion efficiency (PCE) over 14% in single junction cells and over 17% in tandem cells. These high performances, together with the suitability of the technology to inexpensive large-scale manufacture, over lightweight and flexible plastic substrates using roll-to-roll (R2R) processing, place the technology amongst the most promising for future harvesting of solar energy. Although OPVs using non-fullerene acceptors have recently outperformed their fullerene-based counterparts, the research in the development of new fullerenes and in the improvement of the bulk-heterojunction (BHJ) morphology and device efficiency of polymer:fullerene solar cells remains very active. In this review article, the most relevant research works performed over the last 3 years, that is, since the year 2016 onwards, in the field of fullerene-based polymer solar cells based on the copolymers PTB7, PTB7-Th (also known as PBDTTT-EFT) and PffBT4T-2OD, are presented and discussed. This review is primarily focused on studies that involve the improvement of the BHJ morphology, efficiency and stability of small active area devices (typically < 15 mm2), through the use of different processing strategies such as the use of different fullerene acceptors, different processing solvents and additives and different thermal treatments.
Research on the Factors Affecting the Growth of Large-Size Monolayer MoS2 by APCVD
Authors: Han Tao , Liu Hongxia , Wang Shulong , Li Wei , Chen Shupeng , Yang Xiaoli , Cai Ming
Published: December 2018

The transition-metal chalcogenides (TMDs) are gaining increased attention from many scientists recently. Monolayer MoS2 is an emerging layered TMD material with many excellent physical and electrical properties. It can be widely used in catalysis, transistors, optoelectronics and integrated circuits. Here, the large-sized monolayer MoS2 is grown on the silicon substrate with a 285-nm-thick oxide layer by atmospheric pressure chemical vapor deposition (APCVD) of sulfurized molybdenum trioxide. This method is simple and it does not require vacuum treatment. In addition, the effects of growth conditions, such as sulfur source, molybdenum source, growth temperature, and argon flow rate on the quality and area of MoS2 are further studied systematically. These analysis results help to master the morphology and optical properties of monolayer MoS2. The high quality, excellent performance, and large-size monolayer MoS2 under the optimal growth condition is characterized by optical microscopy, AFM, XPS, photoluminescence, and Raman spectroscopy. The Raman spectrum and PL mapping show that the grown MoS2 is a uniform triangular monolayer with a side length of 100 μm, which can pave the way for the applications of photodetectors and transistors.
Bromopyrrole Alkaloids from the Sponge Agelas kosrae
Authors: Kwon Oh-Seok , Kim Donghwa , Kim Heegyu , Lee Yeon-Ju , Lee Hyi-Seung , Sim Chung , Oh Dong-Chan , Lee Sang , Oh Ki-Bong , Shin Jongheon
Published: December 2018

Two new sceptrin derivatives (1,2) and eight structurally-related known bromopyrrole-bearing alkaloids were isolated from the tropical sponge Agelas kosrae. By a combination of spectroscopic methods, the new compounds, designated dioxysceptrin (1) and ageleste C (2), were determined to be structural analogs of each other that differ at the imidazole moiety. Dioxysceptrin was also found to exist as a mixture of α-amido epimers. The sceptrin alkaloids exhibited weak cytotoxicity against cancer cells. Compounds 1 and 2 also moderately exhibited anti-angiogenic and isocitrate lyase-inhibitory activities, respectively.