bluuebunny/crossref_metadata_2025 huggingface dataset:
Co-application of biochars and Piriformospora indica improved the quality of coastal saline soil and promoted the growth of forage
Authors: Wu Qicong , Ning Ke , Liu Bingqian , Zheng Xuejia , Li Chen , Li Xin , Zhou Xiaohu , Li Jiawang , Li Jiajing , Zhang Congzhi , Dong Zhi
Published: August 2024

Soil quality is defined as the ability of soil to maintain the soil environment and the biosphere. Due to the limitation of salt and alkali stress, soil quality can be reduced, which in turn affects agricultural production. Biochar is widely used in saline–alkali land improvement because of its special pore structure and strong ion exchange ability, while Piriformospora indica is widely used in saline–alkali land improvement because it can symbiose with plants and improve plant stress resistance. However, the synergistic effect of combined biochar application and inoculation of P. indica on the quality of saline–alkali soil and plant development is uncertain. Hence, we investigated the combined influences of biochar and P. indica on the soil physicochemical characteristics, as well as the growth and chlorophyll florescence of sorghum–sudangrass hybrids (Sorghum bicolor × Sorghum sudane) in our study. The results indicated that after applying biochar and P. indica together, there was a considerable drop in soil pH, conductivity, Na+, and Cl− concentrations. Meanwhile, the soil organic matter (SOM), available phosphorus (AP), and alkaline hydrolyzable nitrogen (AN) increased by 151.81%, 50.84%, and 103.50%, respectively, when the Bamboo biochar was combined with 120 ml/pot of P. indica. Eventually, sorghum–sudangrass hybrid biomass, transpiration rate, and chlorophyll content increased by 111.69%, 204.98%, and 118.54%, respectively. According to our findings, using P. indica and biochar together can enhance soil quality and plant growth. The results also provide insights to enhance the quality of saline–alkali soils and the role of microorganisms in nutrient cycling.
Patients’ perspective on the therapeutic relationship and session quality: the central role of alliance
Authors: Stefana Alberto , Fusar-Poli Paolo , Vieta Eduard , Youngstrom Eric A.
Published: August 2024

In this study, we examined how four components of the therapeutic relationship—working alliance, real relationship, and positive and negative affective reactions of the patient toward their therapist—relate to each other and to the psychotherapy session outcome, from the patient’s point of view. Our simple comprised 700 adult patients in individual psychotherapy who were recruited and participated online. They underwent a baseline evaluation of their most recent therapy session, which encompassed a series of validated self-report measures focused on specific elements of the therapeutic relationship. The results revealed that, from the patient’s perspective, working alliance, real relationship, and positive affective reactions toward the therapist were positively correlated with session outcome, while negative affective reactions were negatively correlated. All components predicted session outcome when simultaneously included in a regression model. Collectively, these four components accounted for 30% of the variance in session outcome. Factor analysis revealed four distinct factors, underlying perceptions of the therapeutic relationship. Notably, the bond dimension of the alliance was sufficiently different from the task and goal dimensions, warranting consideration as a distinct construct. These findings, although cross-sectional, lay the groundwork for a more nuanced investigation of multiple dimensions of the therapeutic relationship.
Is it possible for people to develop a sense of empathy toward humanoid robots and establish meaningful relationships with them?
Authors: Morgante Elena , Susinna Carla , Culicetto Laura , Quartarone Angelo , Lo Buono Viviana
Published: August 2024

Introduction

Empathy can be described as the ability to adopt another person’s perspective and comprehend, feel, share, and respond to their emotional experiences. Empathy plays an important role in these relationships and is constructed in human–robot interaction (HRI). This systematic review focuses on studies investigating human empathy toward robots. We intend to define empathy as the cognitive capacity of humans to perceive robots as equipped with emotional and psychological states.

Methods

We conducted a systematic search of peer-reviewed articles using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. We searched Scopus, PubMed, Web of Science, and Embase databases. All articles were reviewed based on the titles, abstracts, and full texts by two investigators (EM and CS) who independently performed data collection. The researchers read the full-text articles deemed suitable for the study, and in cases of disagreement regarding the inclusion and exclusion criteria, the final decision was made by a third researcher (VLB).

Results

The electronic search identified 484 articles. After reading the full texts of the selected publications and applying the predefined inclusion criteria, we selected 11 articles that met our inclusion criteria. Robots that could identify and respond appropriately to the emotional states of humans seemed to evoke empathy. In addition, empathy tended to grow more when the robots exhibited anthropomorphic traits.

Discussion

Humanoid robots can be programmed to understand and react to human emotions and simulate empathetic responses; however, they are not endowed with the same innate capacity for empathy as humans.
Serious leisure and successful aging among elderly air volleyball players: examining the mediating role of social support and flow experience
Authors: Wang Jingzhong , Tian Haibo
Published: August 2024

Introduction

Previous literature has demonstrated that engagement in serious leisure (SL) is associated with subjective well-being among older adults, while the relationship between successful aging (SA) and SL remains unexplored. This study aims to investigated the association between SL, social support (SS), flow experience (FE), and SA.

Methods

A total of 435 older adults participating in air volleyball events were included in this study.

Results

The findings revealed that: (i) SL directly and positively influences on SS, FE, and SA; (ii) SS is positively related to FE, and FE is positively associated with older adults’SA; (iii) Both SS and FE fully mediate the relationship between SL and SA, with SS partially explaining this mediation through FE.

Discussion

This study builds upon prior research in this field and highlights the significance of SL for the SA among older adults. Future studies should further explore the underlying mechanisms linking serious sport experiences to successful elderly life.
Electroconvulsive therapy modulates loudness dependence of auditory evoked potentials: a pilot MEG study
Authors: Dib Michael , Lewine Jeffrey David , Abbott Christopher C. , Deng Zhi-De
Published: August 2024

Introduction

Electroconvulsive therapy (ECT) remains a critical intervention for treatment-resistant depression (MDD), yet its neurobiological underpinnings are not fully understood. This pilot study aims to investigate changes in loudness dependence of auditory evoked potentials (LDAEP), a proposed biomarker of serotonergic activity, in patients undergoing ECT.

Methods

High-resolution magnetoencephalography (MEG) was utilized to measure LDAEP in nine depressed patients receiving right unilateral ECT. We hypothesized that ECT would reduce the LDAEP slope, reflecting enhanced serotonergic neurotransmission. Depression severity and cognitive performance were assessed using the 24-item Hamilton Depression Rating Scale (HDRS24) and the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS), respectively.

Results

Contrary to our hypothesis, findings indicated a significant increase in LDAEP post-ECT (t8 = 3.17, p = .013). The increase in LDAEP was not associated with changes in depression severity or cognitive performance.

Discussion

The observed increase in LDAEP suggests a more complex interaction between ECT and neurobiological systems, rather than a direct reflection of serotonergic neurotransmission. Potential mechanisms for this increase include ECT’s impact on serotonergic, dopaminergic, glutamatergic, and GABAergic receptor activity, neuroplasticity involving brain-derived neurotrophic factor (BDNF), and inflammatory modulators such as TNF-α. Our results highlight the multifaceted effects of ECT on brain function, necessitating further research to elucidate these interactions.
A mobile app-based intervention improves anthropometry, body composition and fitness, regardless of previous active-inactive status: a randomized controlled trial
Authors: Gómez-Cuesta Nerea , Mateo-Orcajada Adrián , Meroño Lourdes , Abenza-Cano Lucía , Vaquero-Cristóbal Raquel
Published: August 2024

Introduction

The use of mobile apps to promote physical activity in adolescents can improve health-related parameters. However, previous studies have not evaluated whether the benefits depend on the users’ prior active or inactive status. Therefore, the main objective was to analyze differences in physical activity levels, adherence to the Mediterranean diet (AMD), anthropometry, body composition, and physical fitness between active and inactive adolescents.

Methods

The study was conducted through a randomized controlled trial (RCT) with 462 adolescents, divided into experimental (EG) and control groups (CG), further categorized as active and inactive. Variables of physical activity, kinanthropometry, body composition, and physical fitness were measured before (pre-test) and after (post-test) a 10-week intervention using step-tracking apps (Strava, Pacer, MapMyWalk, and PokémonGo) at least three times per week.

Results

The results showed that inactive EG adolescents significantly increased their physical activity levels, body mass, and muscle mass, and improved in all fitness variables except the countermovement jump (CMJ). The sum of three skinfolds also significantly decreased. Active EG adolescents increased body and muscle mass and improved in all fitness variables. Additionally, they significantly reduced fat mass and the sum of three skinfolds. All covariates, mainly gender and maturity, had significant effects on the study variables. Comparing changes between the active EG and CG groups, significant differences were found in body mass index (BMI) and CMJ in favor of the EG. However, while significant differences were observed in the study variables when analyzing each app individually, there were no differences between the changes produced by each app in these variables.

Conclusion

After a 10-week program of physical activity promoted through step-tracking apps, improvements were observed in fat variables, cardiorespiratory fitness, and curl-up performance. Furthermore, only inactive adolescents perceived an increase in their level of physical activity. The measurement protocol was registered prior to the start of the intervention at ClinicalTrials.gov (code: NCT04860128).
Impact of recruitment strategies on individual participation practices in the Canadian National Vaccine Safety Network: prospective cohort study
Authors: Soe Phyumar , Sadarangani Manish , Naus Monika , Muller Matthew P. , Vanderkooi Otto G. , Kellner James D. , Top Karina A. , Wong Hubert , Isenor Jennifer E. , Marty Kimberly , De Serres Gaston , Valiquette Louis , McGeer Allison , Bettinger Julie A. ,   for the Canadian Immunization Research Network
Published: August 2024

Background

The Canadian National Vaccine Safety (CANVAS) network conducted a multi-center, prospective vaccine safety study to collect safety data after dose 1 and 2 of COVID-19 vaccines and follow up safety information 7 months after dose 1.

Objective

This study aimed to describe and evaluate the recruitment methods used by CANVAS and the retention of participants by each modality.

Methods

CANVAS deployed a multi-pronged recruitment approach to reach a larger sample, without in-person recruitment. Three primary recruitment strategies were used: passive recruitment, technology-assisted electronic invitation through the vaccine booking system (auto-invitation), or auto-registration through the vaccine registries (auto-enrollment).

Results

Between December 2020 and April 2022, approximately 1.3 million vaccinated adults either self-enrolled or were auto-enrolled in CANVAS, representing about 5% of the vaccinated adult Canadian population. Approximately 1 million participants were auto-enrolled, 300,000 were recruited by auto-invitation, and 5,000 via passive recruitment. Overall survey completion rates for dose 1, dose 2 and the 7-month follow-up surveys were 51.7% (681,198 of 1,318,838), 54.3% (369,552 of 681,198), and 66.4% (452,076 of 681,198), respectively. Completion rates were lower among auto-enrolled participants compared to passively recruited or auto-invited participants who self-enrolled. However, auto-enrolled samples were much larger, which offset the lower completion rates.

Conclusion

Our data suggest that auto-enrollment provided an opportunity to reach and retain a larger number of individuals in the study compared to other recruitment modalities.
Promising behavior change techniques for climate-friendly behavior change – a systematic review
Authors: Masciangelo Lisa , Lopez Lumbi Susanne , Rinderhagen Michel , Hornberg Claudia , Liebig-Gonglach Michaela , Mc Call Timothy
Published: August 2024

Introduction

Besides societal and governmental actions to mitigate greenhouse gases, individual behavioral changes are also urgently needed to limit global temperature rise. However, these individual changes have proven to be difficult to achieve in the general population.

Methods

We conducted a systematic review in five electronic databases with the aim of systematically depicting the content of interventions that promote climate-friendly behavior in individuals and households in high- and upper-middle-income countries.

Results

We included 25 studies. The analyses included identification of the used Behavior Change Techniques (BCTs) and comparison of their promise ratio. Across our three outcome categories energy consumption, water consumption, and mobility the most frequently used BCT categories are not the ones that are most promising in terms of behavior change.

Discussion

Based on these results, our recommendation for climate change mitigation interventions is to include components that provide concrete instructions on how to perform the desired behavior (shaping knowledge), setting goals and commitments (goals and planning), substituting undesired behavior, and practicing desired behavior (repetition and substitution). Other reviews with similar aims use different wordings, definitions, or degrees of detail in their intervention component labelling which makes it difficult to compare the results. We recommend to use a standardized classification system, like the BCT taxonomy in combination with the promise ratio, which this study has shown to be a suitable tool to classify applied intervention techniques and give an indication of successful techniques.
Association of blood cadmium, lead, and mercury with anxiety: a cross-sectional study from NHANES 2007–2012
Authors: Bai Long , Wen Zongliang , Zhu Yan , Jama Hamdi Abdirizak , Sawmadal Joyce D. , Chen Jialin
Published: August 2024

Objectives

The purpose of this paper is to explore the relationship between blood levels of cadmium, lead, and mercury and anxiety in American adults.

Methods

Blood metals and self-reported anxiety days were extracted from laboratory data and questionnaire data, respectively, using NHANES data from 2007–2012. Weighted logistic regression was used to assess the relationship between cadmium, lead and mercury with anxiety. Restricted cubic spline was used to visualize the non-linear relationship between metal concentrations and anxiety. Weighted quantile sum (WQS) regression was used to investigate the effect of combined exposure to the three metals on anxiety.

Results

The prevalence of anxiety in adults was 26.0%. After adjusting for potential confounding variables, cadmium levels in the highest quartile (Q4) were associated with a higher risk of anxiety compared to the lowest quartile (Q1) (OR = 1.279, 95% CI: 1.113–1.471, p < 0.01). Restricted cubic spline analysis indicated a positive association between blood cadmium levels and anxiety. Furthermore, co-exposure to multiple heavy metals was positively associated with anxiety risk (WQS positive: OR = 1.068, 95% CI: 1.016–1.160, p < 0.05), with cadmium contributing the most to the overall mixture effect. Compared to the Light RPA, the Vigorous/Moderate RPA group had a relatively low risk of anxiety after cadmium exposure.

Conclusion

High levels of blood cadmium are positively associated with the development of anxiety disorders, which needs to be further verified in future studies.
The mediator role of the feeling of personal unaccomplishment in the effect of unemployment anxiety on depression: a research on business faculty students in Türkiye
Authors: Yumusak Ibrahim Güran
Published: August 2024

Background

This study aims to determine how the unemployment anxiety of university students affects their depression level. It also examines the mediating role of the feeling of personal unaccomplishment between these effects.

Methods

The data was analyzed using the PROCESS method. The research was conducted on 843 students attending different public or foundation (private) universities in Türkiye. Random sampling was used to select the participants.

Results

As a result of the analysis, it was determined that there is a positive, significant, moderate (R = 0.509, p < 0.01) relationship between unemployment anxiety and depression and a positive, significant and weak relationship between unemployment anxiety and feeling of personal unaccomplishment (R = 0.102, p < 0.01), there is a positive, significant, and weak (R = 0.184, p < 0.01) relationship between feeling of personal unaccomplishment and depression. Unemployment anxiety of university students had significant and positive effects on their depression level and significant and positive effects on feelings of personal unaccomplishment of students.

Conclusion

As a result of the mediating variable analysis, it was determined that the feeling of personal unaccomplishment has a partial mediator role in the relationship between unemployment anxiety and depression.
Double-duty actions addressing the double burden of malnutrition among adolescents aged 10–19: protocol for a scoping review
Authors: Zhao Shuangyu , Shinde Sachin , Mastan Melinda , Fawzi Wafaie , Tang Kun
Published: August 2024

Background

The global adolescent population faces the challenge of the double burden of malnutrition (DBM), characterized by the coexistence of undernutrition and overweight/obesity, including diet-related non-communicable diseases (NCDs). This dual challenge, prevalent across various socio-economic backgrounds, necessitates double-duty actions, i.e., integrated interventions designed to concurrently address both sets of conditions. These actions are critical for fostering the overall health and well-being of adolescents. The objective of this review is to describe the content, setting, and delivery mechanisms of double-duty actions, synthesize their impacts on adolescents’ nutritional status, and offer policy and program implications for future interventions.

Methods

As part of this scoping review, we will conduct a comprehensive search across multiple databases, including MEDLINE, Embase, CENTRAL, CINAHL, and Google Scholar, to identify relevant interventions, programs, policies, guidelines, evaluation studies, and strategies targeting DBM among adolescents aged 10–19 years. Inclusion criteria encompass a range of evidence sources with methodologically sound and well-described study designs. All full-text articles and abstracts will be independently screened by two reviewers to ensure a comprehensive inclusion of papers that align with the established criteria. The final protocol is available on Open Science Forum (https://osf.io/kxapb).

Discussion

Addressing DBM through integrated double-duty actions is crucial for achieving global nutrition and public health goals. However, challenges persist in the form of uncoordinated efforts, lack of comprehensive evidence for what interventions work among adolescents, and the need for context-specific strategies to effectively address the heterogeneity of DBM. The results of this scoping review may provide evidence for future policies and interventions, emphasizing integrated, multi-sectoral strategies tailored to the unique needs of the adolescent population.
Comprehensive analysis of classroom microclimate in context to health-related national and international indoor air quality standards
Authors: Caciora Tudor , Ilieş Alexandru , Berdenov Zharas , Al-Hyari Hadeel Sa'ad , Ilieş Dorina Camelia , Safarov Bahodirhon , Hassan Thowayeb H. , Herman Grigore Vasile , Hodor Nicolaie , Bilalov Bahadur , Peres Ana Cornelia
Published: August 2024

Indoor air quality (IAQ) and indoor air pollution are critical issues impacting urban environments, significantly affecting the quality of life. Nowadays, poor IAQ is linked to respiratory and cardiovascular diseases, allergic reactions, and cognitive impairments, particularly in settings like classrooms. Thus, this study investigates the impact of indoor environmental quality on student health in a university classroom over a year, using various sensors to measure 19 environmental parameters, including temperature, relative humidity, CO2, CO, volatile organic compounds (VOCs), particulate matter (PM), and other pollutants. Thus, the aim of the study is to analyze the implications of the indoor microclimate for the health of individuals working in the classroom, as well as its implications for educational outcomes. The data revealed frequent exceedances of international standards for formaldehyde (HCHO), VOC, PM2.5, NO, and NO2. HCHO and VOCs levels, often originating from building materials and classroom activities, were notably high. PM2.5 levels exceeded both annual and daily standards, while NO and NO2 levels, possibly influenced by inadequate ventilation, also surpassed recommended limits. Even though there were numerous exceedances of current international standards, the indoor microclimate quality index (IMQI) score indicated a generally good indoor environment, remaining mostly between 0 and 50 for this indicator. Additionally, analyses indicate a high probability that some indicators will exceed the current standards, and their values are expected to trend upwards in the future. The study highlighted the need for better ventilation and pollutant control in classrooms to ensure a healthy learning environment. Frequent exceedances of pollutant standards can suggest a significant impact on student health and academic performance. Thus, the present study underscored the importance of continuous monitoring and proactive measures to maintain optimal indoor air quality.
Lifestyles and academic stress among health sciences students at the National University of Chimborazo, Ecuador: a longitudinal study
Authors: Salazar-Granizo Yolanda E. , Hueso-Montoro César , Caparros-Gonzalez Rafael A.
Published: August 2024

Background

The significant changes experienced by university students in their training are inherent to educational processes. Social isolation caused by the COVID-19 pandemic, online education and the reopening of higher-education institutions produced substantial variations in the lifestyle of university students in health sciences and generated academic stress and perceived stress. This study was conducted at the National University of Chimborazo (UNACH), a public institution located in Riobamba, central Ecuador, the diverse student's population provided an ideal setting for examining the interplay between academic and perceived stress, lifestyle factors, and learning modalities. The research focused on health sciences students across six academic programs. The university's geographical position and demographic characteristics offered a representative sample for investigating these factors within the context changing.

Aim

To compare academic and perceived stress and university students' lifestyles at two different periods: (1) during the mandatory social confinement caused by the COVID-19 pandemic with an online learning modality (T1); (2) in the post-pandemic period with a return to face-to-face activities (T2).

Design

An observational, analytical, quantitative, and longitudinal study.

Participants

Students from six programs (Nursing, Physiotherapy, Clinical Laboratory, Medicine, Dentistry, and Clinical Psychology) from the Faculty of Health of the National University of Chimborazo-Ecuador (n = 2,237) participated voluntarily, the students had one mean age of M = 21.31 (SD = 2.36) at T1 and M = 22.94 (SD = 2.40) at T2. Non-probability convenience sampling was employed due to the accessibility of the student population and the importance of including the maximum number of relevant individuals within the study population.

Methods

The following instruments were used: Nola Pender's Lifestyle Profile Questionnaire, Cognitive Systemic Inventory for the study of academic stress, and Cohen's Perceived Stress Scale.

Results

In T1 and T2, students reported high levels of stress, and increased unhealthy lifestyle increased with the return to classrooms. Additionally, upon returning to face-to-face activities (T2), the mean score applied to the responses of Nola Pender's Lifestyle Profile Questionnaire decreased from M = 113.34 (SD = 23.02) to M = 107.2 (SD = 29.70; p < 0.001). There was significant difference (p < 0.001) in academic stress in T1 [M = 66.25 (SD = 15.66)] and T2 [M = 64.00 (SD = 17.91)].

Conclusions

Upon returning to university classrooms (T2), the number of students who reported an “unhealthy” lifestyle increased. Academic stress was high in T1 and T2 and was reported higher in online activities during social isolation.
Implementing social and affective touch to enhance user experience in human-robot interaction
Authors: Cansev M. Ege , Miller Alexandra J. , Brown Jeremy D. , Beckerle Philipp
Published: August 2024

In this paper, we discuss the potential contribution of affective touch to the user experience and robot performance in human-robot interaction, with an in-depth look into upper-limb prosthesis use as a well-suited example. Research on providing haptic feedback in human-robot interaction has worked to relay discriminative information during functional activities of daily living, like grasping a cup of tea. However, this approach neglects to recognize the affective information our bodies give and receive during social activities of daily living, like shaking hands. The discussion covers the emotional dimensions of affective touch and its role in conveying distinct emotions. In this work, we provide a human needs-centered approach to human-robot interaction design and argue for an equal emphasis to be placed on providing affective haptic feedback channels to meet the social tactile needs and interactions of human agents. We suggest incorporating affective touch to enhance user experience when interacting with and through semi-autonomous systems such as prosthetic limbs, particularly in fostering trust. Real-time analysis of trust as a dynamic phenomenon can pave the way towards adaptive shared autonomy strategies and consequently enhance the acceptance of prosthetic limbs. Here we highlight certain feasibility considerations, emphasizing practical designs and multi-sensory approaches for the effective implementation of affective touch interfaces.
Reducing vulnerability to climate change among millet and sorghum farmers in Ghana: interrogating the contribution of climate-smart agriculture in northwestern Ghana
Authors: Yiridomoh Gordon Yenglier , Bonye Samuel Ziem , Derbile Emmanuel K.
Published: August 2024

Reducing vulnerability of smallholder farmers to climate change is a global issue. One approach viewed as important in reducing farmers’ vulnerability to climate change is Climate-Smart Agriculture (CSA). CSA is often seen as an approach to redefine, reposition and sustainably manage agriculture. Given the importance of CSA practices in sustaining the food needs of many farm households in sub-Saharan Africa and Ghana, this study investigates CSA practices that were introduced to farmers by Center for Indigenous Knowledge and Development (CIKOD), interrogates the contributions of CSA to reducing farmers vulnerability to climate change and established the relationship between CSA and climate change adaptation. The study employed a mixed method approach, using 146 smallholder millet and sorghum farmers. Questionnaire and interviews were used to generate primary data for analysis. Descriptive statistics, involving Chi-square test and relative importance index were used to analyze the questionnaire while thematic analytical approach was used to analyze the interviews. The results of the study revealed that CSA practices such as crop rotation, weed control, contour farming, and land rotation are deployed by smallholder farmers to respond to drought, dry spell and flood in the Municipality. Asset holding capacity, credit, access to climate information, and extension services were found to be key determinants of farmers’ adoption of CSA practices. The study recommends the need for the Ministry of Food and Agriculture to provide some technical support to smallholder farmers to successfully adopt these practices for sustainable farming. Again, the study recommends the need for non-governmental organizations and development partners, which over the years have shown interest in promoting CSA practices among farmers, to continuous to support and promote the adoption of CSA by farmers.
Pretreatment of the urethral mucosa at the tip of the prostate: a retrospective review in preventing stress urinary incontinence after thulium laser enucleation of the prostate
Authors: Liao Yunfeng , Wu Yuting , Zou Junrong , Huang Ruohui , Xia Wei , Yuan Yuanhu , Xiao Rihai , Wang Xiaoning , Wu Gengqing , Zou Xiaofeng , Zhang Guoxi
Published: August 2024

Objective

Explore the clinical application value of urethral mucosal pretreatment at the tip of the prostate in preventing stress urinary incontinence (SUI) after thulium laser enucleation of the prostate (ThuLEP).

Methods

Eighty-seven patients with benign prostatic hyperplasia (BPH) treated with ThuLEP from June 2021 to December 2022 were divided into two groups. Of these, 42 patients (group A) underwent conventional ThuLEP and 45 patients (group B) were enucleated after pretreatment of the urethral mucosa. At the tip of the prostate, pretreatment of the urethral mucosa consisted of pushing the gland separately on both sides at the level of the verumontanum and cutting off the mucosa near the external urethral sphincter clockwise and counterclockwise. The perioperative and postoperative follow-up indicators [operation time, hemoglobin reduction, complications, Qmax, International Prostate Symptom Score (IPSS), quality of life (QoL), and post-void residual (PVR) volume] of the two groups of patients were collected and compared. All patients were followed up 1 month after surgery.

Results

All 87 procedures were successfully completed. There was no significant difference in age and gland size between the two groups (P > 0.05). There was no significant difference between operating time and hemoglobin reduction in the two groups (P > 0.05). The Qmax, IPSS, QOL, and PVR volume were significantly improved postoperatively in both groups (P < 0.05). Temporary SUI occurred in both groups [12 cases (28.5%) in group A and 3 cases (6.7%) in group B (P < 0.05)]. There was no significant difference in the incidence of infection and urethral stricture between the two groups (P > 0.05).

Conclusion

Pretreatment of the urethral mucosa before ThuLEP for BPH significantly reduces the incidence of SUI after surgery. This technique, which preconditions the apical urethral mucosa of the prostate, is safe and effective, has few complications, and is worthy of clinical application.
Host–microbe interactions in the nasal cavity of dogs with chronic idiopathic rhinitis
Authors: Wang Zhe , Chow Lyndah , Das Sunetra , Impastato Renata , Manchester Alison C. , Dow Steven
Published: August 2024

Chronic rhinitis (CR) is a frustrating clinical syndrome in dogs and our understanding of the disease pathogenesis in is limited. Increasingly, host–microbe interactions are considered key drives of clinical disease in sites of persistent mucosal inflammation such as the nasal and oral cavities. Therefore, we applied next generation sequencing tools to interrogate abnormalities present in the nose of dogs with CR and compared immune and microbiome profiles to those of healthy dogs. Host nasal cell transcriptomes were evaluated by RNA sequencing, while microbial communities were assessed by 16S rRNA sequencing. Correlation analysis was then used to identify significant interactions between nasal cell transcriptomes and the nasal microbiome and how these interactions were altered in animals with CR. Notably, we observed significant downregulation of multiple genes associated with ciliary function in dogs with CR, suggesting a previously undetected role for ciliary dysfunction in this syndrome. We also found significant upregulation of immune genes related to the TNF-α and interferon pathways. The nasal microbiome was also significantly altered in CR dogs, with overrepresentation of several potential pathobionts. Interactome analysis revealed significant correlations between bacteria in the genus Porphyromonas and the upregulated host inflammatory responses in dogs with CR, as well as defective ciliary function which was correlated with Streptococcus abundance. These findings provide new insights into host–microbe interactions in a canine model of CR and indicate the presence of potentially causal relationships between nasal pathobionts and the development of nasal inflammation and ciliary dysfunction.
Synthetic antimicrobial peptides Bac-5, BMAP-28, and Syn-1 can inhibit bovine respiratory disease pathogens in vitro
Authors: Cornejo Santiago , Barber Cassandra , Thoresen Merrilee , Lawrence Mark , Seo Keun Seok , Woolums Amelia
Published: August 2024

Mass treatment with antibiotics at arrival has been the mainstay for bovine respiratory disease (BRD) control but there is an increase in antimicrobial-resistant bacteria being shed from treated cattle. BRD is a disease complex that results from the interaction of viruses or bacteria and susceptible animals with inappropriate immunity. With bacteria being the only feasibly treatable agent and the emergence of antimicrobial resistance, decreased efficacy of commonly used antibiotics could threaten livestock health. There is a need for new antimicrobial alternatives that could be used to control disease. Naturally occurring antimicrobial peptides (AMP) have been proposed to address this need. Here we tested the effect of bovine myeloid antimicrobial peptide-28 (BMAP-28), a synthetic BMAP-28 analog Syn-1, and bactenecin 5 (Bac-5) on Mannheimia haemolytica (Mh) using a quantitative culture method and the broth microdilution method to determine minimum inhibitory and bactericidal concentrations (MIC and MBC). We also tested the antiviral effect of these AMP against bovine herpes-1 (BHV-1) and bovine respiratory syncytial virus (BRSV) using the Reed and Muench method to calculate the viral titers after treatment. We demonstrated that BMAP-28 and Syn-1 can inhibit Mh growth and BMAP-28 can inhibit replication of BHV-1 and BRSV. Moreover, we showed that BMAP-28 and Bac-5 can be used together to inhibit Mh growth. When used alone, the MIC of BMAP-28 and Bac-5 was 64 and 128 μg/mL respectively, but when applied together, their MIC ranged from 0.25–16 for BMAP-28 and 8–64 μg/mL for Bac-5, resulting in a decrease in concentration of up to 256 and 16-fold, respectively. The synergistic interaction between those peptides resulted in concentrations that could be well tolerated by cells. Our results demonstrate that bovine cathelicidins could be used as alternatives to antimicrobials against BRD pathogens. These findings introduce a path to discovering new antimicrobials and determining how these peptides could be tailored to improve cattle health.
Research Progress on the Mechanism of Histone Deacetylases in Ferroptosis of Glioma
Authors: Ma Meng , Fei Xifeng , Jiang Dongyi , Chen Hanchun , Xie Xiangtong , Wang Zhimin , Huang Qiang
Published: August 2024

Glioma is the most prevalent primary malignant tumor of the central nervous system. While traditional treatment modalities such as surgical resection, radiotherapy, and chemotherapy have made significant advancements in glioma treatment, the prognosis for glioma patients remains often unsatisfactory. Ferroptosis, a novel form of programmed cell death, plays a crucial role in glioma and is considered to be the most functionally rich programmed cell death process. Histone deacetylases have emerged as a key focus in regulating ferroptosis in glioma. By inhibiting the activity of histone deacetylases, histone deacetylase inhibitors elevate acetylation levels of both histones and non-histone proteins, thereby influencing various cellular processes. Numerous studies have demonstrated that histone deacetylases are implicated in the development of glioma and hold promise for its treatment. This article provides an overview of research progress on the mechanism by which histone deacetylases contribute to ferroptosis in glioma.
Investigation of the Overall Damage Assessment Method Used for Unmanned Aerial Vehicles Subjected to Blast Waves
Authors: Feng Xiaowei , Yang Zezhou , Nie Yuan
Published: August 2024

With the aim of investigating the assessment methodology for the overall damage effects on an unmanned aerial vehicle (UAV) subjected to a blast wave, the failure criteria for the typical UAV was formulated through an analysis of the structural strength design standards. Specifically, the shear force associated with wing failure can serve as a critical parameter for assessing the overall damage inflicted on the UAV by blast waves. According to the design load criterion of the aircraft, the shear force value corresponding to the overall failure of the typical UAV was calculated. Numerical simulations were conducted to investigate the mechanical response of UAV structures under blast wave loading generated by a 500 g explosive. Combining the critical shear force values obtained from theoretical calculations with the numerical simulation results, two distances between the explosives and the UAV that could produce different damage effects were estimated, namely 1 m and 2.5 m. Subsequently, static explosion experiments with equivalent explosive charges were performed, revealing different damage effects on a typical UAV at two specific distances. The numerical simulation results were highly consistent with the experimental observations, further validating the scientific and rational basis for using shear force as a primary parameter in assessing overall structural damage to fixed-wing UAVs.
Time Optimal Altitude-Hold Flight Mode Transition Strategy for a Class of Ducted Fan Tail Sitter UAV
Authors: Cheng Zihuan , Pei Hailong
Published: August 2024

For special tail sitter configurations such as the ducted fan tail sitter unmanned aerial vehicle (UAV), the widely used trajectory planning methodology based on differential flatness might not be applicable due to complex aerodynamic coupling effects. As a result, the flight mode transition remains a challenging task. In this paper, we address the time optimal altitude-hold flight mode transition issue for a class of ducted fan tail sitter UAV. The foundation of the framework is the dynamic transition corridor in which the limitation of jerk is particularly considered, aiming to thoroughly reflect the dynamic feature of aggressive maneuvers. Based on this, we propose a time optimal strategy to generate feasible altitude-hold transition trajectories. Simultaneous, by fully utilizing the manifestation of time optimal altitude-hold flight behavior revealed by the transition corridor, we try to tackle the time optimal altitude-hold transition by means of a novel model-free control scheme. Comparative simulations show that both of the transition strategies achieve satisfactory performance on time optimal altitude-hold transition in the absence of disturbance, while the model-free control scheme exhibits better robustness under external disturbance.
Stability of Single-Channel Homing Rolling Aerospace Vehicle
Authors: Chelaru Teodor-Viorel , Constantinescu Cristian Emil , Pană Valentin , Ene Costin , Chelaru Adrian
Published: August 2024

This paper aims to analyze the stability of a special class of single-channel slow-rotating homing missiles using the Frank–Wall stability criterion. To achieve this, starting from the model of a slow-rolling missile with six degrees of freedom (6 DOFs) in the body frame, a 6-DOFs model in the Resal frame is obtained, which is used to linearize the coupled commanded motion. Based on the linearized model, we obtain the structural scheme of the commanded object and define the flight quality parameters. The obtained linear model has a complex representation (with real and imaginary parts) due to the coupling between longitudinal channels for the rolling missile. Then, the kinematic guidance equations, the seeker equations and the actuator relations using a switching function, specific to the slow-rolling single-channel missile, are defined. The guidance kinematic equations, the seeker equations and the actuator model are linearized in the Resal frame, and the structural diagram of the homing missile is constructed. Starting from this, the characteristic polynomial having complex coefficients is determined and then analyzed with the Frank–Wall stability criterion. Based on the analysis, a stability range is determined for the navigation constant (k), and a minimum and possibly a maximum limit for the time to hit the target tgo is obtained. The stability range defined for the navigation constant in the linear model is finally validated in the nonlinear model in the body frame.
Analysis of BnGPAT9 Gene Expression Patterns in Brassica napus and Its Impact on Seed Oil Content
Authors: Xing Man , Hong Bo , Lv Mengjie , Lan Xueyi , Zhang Danhui , Shu Chunlei , Qi Shucheng , Peng Zechuan , Guan Chunyun , Xiong Xinghua , Huang Luyao
Published: August 2024

Glycerol-3-phosphate acyltransferase (GPAT) genes encode enzymes involved in the biosynthesis of plant oils. Rapeseed has four BnGPAT9 genes, but the expression patterns and functions of these four homologous copies in rapeseed for seed oil accumulation are not well understood. In this study, we cloned the four BnGPAT9 genes and their promoters from Brassica napus and found significant differences in the expression of BnGPAT9 genes among different rapeseed varieties. We confirmed that BnGPAT9-A01/C01 are highly conserved in rapeseed, with high expression levels in various tissues, especially during the late stages of silique development and seed maturation. All four BnGPAT9 genes (BnGPAT9-A01/C01/A10/C09) can promote seed oil accumulation, but BnGPAT9-A01/C01 have a greater effect. Overexpression in Arabidopsis and rapeseed increased seed oil content and altered fatty acid composition, significantly increasing linolenic acid content. Transcriptome analysis revealed that BnGPAT9 genes promote the upregulation of genes related to oil synthesis, particularly those in the Plant–pathogen interaction, alpha-Linolenic acid metabolism, MAPK signaling pathway—plant, and Glutathione metabolism pathways. In summary, these results indicate that the four BnGPAT9 genes in rapeseed have different expression patterns and roles in regulating seed oil accumulation, with BnGPAT9-A01/C01 contributing the most to promoting oil accumulation.
Chlorophyll Fluorescence Imaging Reveals the Dynamics of Bentazon Action on Sunflower (Helianthus annuus L.) Plants
Authors: Bazhenov Mikhail , Litvinov Dmitry , Kocheshkova Alina , Karlov Gennady , Divashuk Mikhail
Published: August 2024

Fluorescence photography was used to evaluate the effects of bentazon, an herbicide that inhibits electron transport in photosystem II, on sunflower, taken as a model plant. We measured chlorophyll fluorescence to visualize the penetration, distribution, and effect of the herbicide on the plant. Our results showed that bentazon penetrates the leaf within 10–16 min, reaching full depth within 40 min. Also, we show that the herbicide is distributed along the conducting tissues of the leaf. The presence of necrotic spots, as well as the size of increased-fluorescence areas, were positively correlated to the concentration of herbicide. These findings suggest that fluorescence imaging may be a useful tool for observing the absorption and distribution of photosystem II-inhibiting herbicides in plants as an alternative to radioactive labeling in some cases.
Evaluation of Pepper (Capsicum spp.) Germplasm Collection for Bacterial Wilt (Ralstonia solanacearum) Resistance
Authors: Ro Nayoung , Haile Mesfin , Oh Hyeonseok , Ko Ho-Cheol , Yi Jungyoon , Na Young-Wang , Hur Onsook
Published: August 2024

This study was conducted to identify resistant pepper accessions against the destructive disease bacterial wilt (BW) caused by Ralstonia solanacearum. A total of 338 pepper (Capsicum species) germplasms collected from different countries and deposited in the National Agrobiodiversity Genebank, Rural Development Administration (RDA), Republic of Korea, were evaluated. The evaluated accessions comprise samples from five distinct species: Capsicum annuum (213), Capsicum baccatum (47), Capsicum chinense (45), Capsicum frutescens (31), and Capsicum chacoense (2). Disease severity scores were recorded over four consecutive weeks and showed an increase in severity from initial inoculation to the end of the evaluation period. A strong correlation was observed between week 1 and 2, as well as between week 3 and 4. Ten resistant pepper accessions were identified. All selected accessions consistently exhibited low disease scores ranging from 0 to 1 throughout the observation period. These accessions belong to C. chinense (2), C. annuum (6), C. chacoense (1), and C. frutescens (1). Accessions such as IT236738 (C. chinense) and IT283498 (C. chinense) were demonstrated to have high resistance, showing no symptoms over all four weeks. Other accessions belonging to C. annuum (IT247232 and IT236340) and C. chacoense (IT158713) maintained a disease score of 0 (no symptoms) for the first three consecutive weeks; however, they developed symptoms with a score of 1 in the fourth week. Other important characteristics of the resistant materials were evaluated, including carotenoids and fruit characteristics. These accessions have important traits beyond resistance to the destructive pepper disease. They will serve as promising resources for breeding resistant pepper varieties against BW to enhance productivity.