bluuebunny/crossref_metadata_2025 huggingface dataset:
Testing of symmetric biphasic stimulation in Vim-DBS ET patients: a randomized-controlled pilot study
Authors: Boogers Alexandra , Peeters Jana , Van Bogaert Tine , De Vloo Philippe , Vandenberghe Wim P. , Nuttin Bart , Mc Laughlin Myles
Published: April 2024

Introduction

Symmetric biphasic pulses have been shown to increase the therapeutic window compared to standard cathodic pulses in ET Vim-DBS patients. Furthermore, three hours of stimulation with biphasic pulses caused less stimulation-induced ataxia compared to cathodic pulses. Therefore, an investigation of the longer-term safety of biphasic pulses is warranted.

Methods

Seven ET patients were included in a randomized double-blind, cross-over design of one week home-use of symmetric biphasic stimulation (anodic phase first) versus cathodic stimulation. Amplitude was set in a double-blinded way, at the tremor arrest threshold. The primary outcome was safety assessed by documenting the adverse events. Secondary outcome parameters were stimulation amplitude, tremor (Fahn-Tolosa-Marin Tremor Rating Scale) and ataxia (International Cooperative Ataxia Rating Scale) severity, quality of life (Quality of Life in Essential Tremor Questionnaire) and cognition (Montreal Cognitive Assessment). Three patients continued in the open-label extension phase for 3 months, during which biphasic stimulation-only was further assessed by the same outcome parameters.

Results

During the 1 week testing, no adverse effects were reported. To obtain equivalent tremor control, the amplitude of the biphasic pulse was significantly higher compared to that of the cathodic pulse (p = 0.003). The other outcome parameters were not significantly different. During the open-label study, one patient used the remote control to increase the amplitude, leading to two falls caused by stimulation-induced ataxia. No other adverse effects occurred.

Discussion and conclusion

In a small cohort, when tested for one week, symmetric biphasic pulses suggest to be safe, but require higher stimulation amplitudes. Further follow-up studies are needed to investigate long-term effects and safety.
Brain functional connectivity in hyperthyroid patients: systematic review
Authors: Tesfaye Ephrem , Getnet Mihret , Anmut Bitew Desalegn , Adugna Dagnew Getnet , Maru Lemlemu
Published: April 2024

Introduction

Functional connectivity (FC) is the correlation between brain regions’ activities, studied through neuroimaging techniques like fMRI. It helps researchers understand brain function, organization, and dysfunction. Hyperthyroidism, characterized by high serum levels of free thyroxin and suppressed thyroid stimulating hormone, can lead to mood disturbance, cognitive impairment, and psychiatric symptoms. Excessive thyroid hormone exposure can enhance neuronal death and decrease brain volume, affecting memory, attention, emotion, vision, and motor planning.

Methods

We conducted thorough searches across Google Scholar, PubMed, Hinari, and Science Direct to locate pertinent articles containing original data investigating FC measures in individuals diagnosed with hyperthyroidism.

Results

The systematic review identified 762 articles, excluding duplicates and non-matching titles and abstracts. Four full-text articles were included in this review. In conclusion, a strong bilateral hippocampal connection in hyperthyroid individuals suggests a possible neurobiological influence on brain networks that may affect cognitive and emotional processing.

Systematic Review Registration

PROSPERO, CRD42024516216.
Brain-age estimation with a low-cost EEG-headset: effectiveness and implications for large-scale screening and brain optimization
Authors: Kounios John , Fleck Jessica I. , Zhang Fengqing , Oh Yongtaek
Published: April 2024

Over time, pathological, genetic, environmental, and lifestyle factors can age the brain and diminish its functional capabilities. While these factors can lead to disorders that can be diagnosed and treated once they become symptomatic, often treatment is difficult or ineffective by the time significant overt symptoms appear. One approach to this problem is to develop a method for assessing general age-related brain health and function that can be implemented widely and inexpensively. To this end, we trained a machine-learning algorithm on resting-state EEG (RS-EEG) recordings obtained from healthy individuals as the core of a brain-age estimation technique that takes an individual's RS-EEG recorded with the low-cost, user-friendly EMOTIV EPOC X headset and returns that person's estimated brain age. We tested the current version of our machine-learning model against an independent test-set of healthy participants and obtained a correlation coefficient of 0.582 between the chronological and estimated brain ages (r = 0.963 after statistical bias-correction). The test-retest correlation was 0.750 (0.939 after bias-correction) over a period of 1 week. Given these strong results and the ease and low cost of implementation, this technique has the potential for widespread adoption in the clinic, workplace, and home as a method for assessing general brain health and function and for testing the impact of interventions over time.
Investigating the role of Epstein-Barr virus and human papillomavirus types 16 and 18 co-infections in cervical disease of Iranian women
Authors: Sadeghi Farzane , Mostaghimi Talieh , Taheri Mahdie , Yazdani Shahla , Javadian Maryam , Ranaee Mohammad , Ghorbani Hossein , Bouzari Zinatossadat , Sadeghi Farzin
Published: April 2024

Introduction

High-risk human papillomaviruses (HR-HPVs) are known to contribute to cervical cancer (CC), but the role of Epstein-Barr virus (EBV) in this process remains unclear, despite EBV’s widespread detection in premalignant and malignant cervical tissues.

Methods

In this cross-sectional study of 258 cervical samples, including both formalin-fixed paraffin-embedded (FFPE) and fresh cervical tissues, the presence and viral load of HR-HPVs (HPV-16 and HPV-18) and EBV were evaluated in Iranian women with cervical intraepithelial neoplasia (CIN), squamous cell carcinoma (SCC), and a cervicitis control group using real-time PCR.

Results

The study revealed a significant correlation between disease severity and both increased HPV-16 positivity and HPV-16 and HPV-18 co-infection (p<0.001). Interestingly, the control group had a higher frequency of EBV-positive cases than SCC/CIN groups (p<0.001). HPV-16 DNA load increased with disease severity (P<0.001), while HPV-18 showed no significant difference (P=0.058). The control group had a higher EBV DNA load compared to SCC/CIN groups (P=0.033). HPV-16 increased the risk of CIN II, CIN III, and SCC, while HPV-18 increased the risk of CIN II and CIN III. Notably, EBV was associated with a lower risk of CIN groups and SCC.

Conclusions

No significant difference in EBV co-infection with HPV-16/18 was found, failing to support the hypothesis that EBV is a cofactor in CC. However, high EBV viral load in the control group suggests a potential “hit and run hypothesis” role in CC progression. This hypothesis suggests that EBV may contribute briefly to the initiation of CC with an initial impact but then becomes less actively involved in its ongoing progression.
Breast cancer insights from Northern Israel: a comprehensive analysis of survival rates among Jewish and Arab women
Authors: Badran Omar , Campisi-Pinto Salvatore , Amna Mahmoud Abu , Turgeman Ilit , Yosef Samih , Bar-Sela Gil
Published: April 2024

This study investigates breast cancer survival rates between 2000 and 2022 in northern Israel, focusing on ethnicity, socioeconomic status, age at diagnosis, and the Charlson Comorbidity Index. Analyzing data from Clalit Health Services, we studied 8,431 breast cancer patients (6,395 Jewish, 2,036 Arab). We compared five- and ten-year survival rates across different demographics. Ethnicity showed a minor impact on survival (OR 1.12, 95% CI: 0.93 - 1.35). Socioeconomic status had a significant effect, with a higher level of improving survival (OR 2.50, 95% CI: 2.04 – 3.08). Age was crucial; women 18-39 had better survival than 60-100, but no significant difference was found between the 18-39 and 40-59 age groups [OR (CI 0.90 – 1.53, p = 0.231)]. For the Charlson Comorbidity Index, women with scores of 3-10 showed lower survival compared to scores of 0 and 1-2. There was a notable improvement in five-year survival rates among patients aged 18-59 diagnosed from 2009-2018 (90.7%) compared to 2000-2008 (86.9%) (p = 0.0046), but not in patients aged 60-100. The study highlights that socioeconomic status, age, and comorbidity scores are significant in determining survival rates for breast cancer. The improvement in survival rates for younger patients diagnosed more recently reflects advancements in treatment and care. This research provides valuable insights into the factors affecting breast cancer survival rates, underscoring the role of socioeconomic status, age, and comorbidities while also highlighting the progress in breast cancer treatment over recent years.
Knowledge mapping and research trends of exosomes in pancreatic cancer: a bibliometric analysis and review (2013-2023)
Authors: Zhou Yongjiang , Feng Jiajie , Wang Qingqing , Zhao Yiwen , Ding Hanyu , Jiang Kexin , Ji Hua , Tang Zheng , Dai Ruiwu
Published: April 2024

Objective

This review aims to provide a quantitative and qualitative bibliometric analysis of literature from 2013 to 2023 on the role of exosomes in PC, with the goal of identifying current trends and predicting future hotspots.

Methods

We retrieved relevant publications concerning exosomes in PC, published between 2013 and 2023, from the Web of Science Core Collection. Bibliometric analyses were conducted using VOSviewer(1.6.19), CiteSpace(6.2.R4), and Microsoft Excel (2019).

Results

A total of 624 papers were analyzed, authored by 4017 researchers from 55 countries/regions and 855 institutions, published in 258 academic journals. China (n=285, 34.42%) and the United States (n=183, 24.87%) were the most frequent contributors and collaborated closely. However, publications from China had a relatively low average number of citations (41.45 times per paper). The output of Shanghai Jiao Tong University ranked first, with 28 papers (accounting for 4.5% of the total publications). Cancers (n=31, 4.9%); published the most papers in this field. Researcher Margot Zoeller published the most papers (n=12) on this topic. Research hotspots mainly focused on the mechanisms of exosomes in PC onset and progression, the role of exosomes in PC early diagnosis and prognosis, exosomes promote the development of PC chemoresistance, and potential applications of exosomes as drug carriers for PC therapies. We observed a shift in research trends, from mechanistic studies toward clinical trials, suggesting that clinical applications will be the focus of future attention. Emerging topics were pancreatic stellate cells, diagnostic biomarkers, mesenchymal stem cells, extracellular vesicles.

Conclusion

Our scientometric and visual analysis provides a comprehensive overview of the literature on the role of exosomes in PC published during 2013–2023. This review identifies the frontiers and future directions in this area over the past decade, and is expected to provide a useful reference for researchers in this field.
First-line immunotherapy efficacy in advanced squamous non-small cell lung cancer with PD-L1 expression ≥50%: a network meta-analysis of randomized controlled trials
Authors: Chen Wei , Liu Hangmei , Li Yiwen , Xue Wenxin , Fan Shuo , Sun Jingbo , Liu Shui , Liu Yang , Zhang Lili
Published: April 2024

Objective

The optimal first-line immunotherapy regimen for patients with PD-L1 expression ≥50% in squamous non-small cell lung cancer (Sq-NSCLC) remains uncertain. This study utilized net-work meta-analysis (NMA) to indirectly compare the efficacy of various first-line immuno-therapy regimens in this patient subset.

Methods

Systematic searches were conducted across PubMed, the Cochrane Library, Web of Science, and Embase databases for randomized controlled trials reporting overall survival (OS) and progression-free survival (PFS) outcomes. The search spanned from database inception to November 3, 2023. Bayesian network meta-analysis was employed for a comprehen-sive analysis. To ensure scientific rigor and transparency, this study is registered in the Interna-tional Prospective Register of Systematic Reviews (PROSPERO) under the registration number CRD42022349712.

Results

The NMA encompassed 9 randomized controlled trials (RCTs), involving 2170 patients and investigating 9 distinct immunotherapy regimens. For OS, the combination of camrelizumab and chemotherapy demonstrated the highest probability (36.68%) of efficacy, fol-lowed by cemiplimab (33.86%) and atezolizumab plus chemotherapy (23.87%). Regarding PFS, the camrelizumab and chemotherapy combination had the highest probability (39.70%) of efficacy, followed by pembrolizumab (22.88%) and pembrolizumab plus chemotherapy (17.69%). Compared to chemotherapy, first-line treatment with immune checkpoint inhibitors (ICIs) in Sq-NSCLC pa-tients exhibited significant improvements in OS (HR 0.59, 95% CI 0.47-0.75) and PFS (HR 0.44, 95% CI 0.37-0.52).

Conclusion

This study suggests that, for Sq-NSCLC patients with PD-L1 expression ≥50%, the first-line immunotherapy regimen of camrelizumab plus chemotherapy provides superior OS and PFS outcomes. Furthermore, ICIs demonstrate enhanced efficacy compared to chemotherapy in this patient population.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier: CRD 42022349712.
Cost-effectiveness of single-visit cervical cancer screening in KwaZulu-Natal, South Africa: a model-based analysis accounting for the HIV epidemic
Authors: Tran Jacinda , Hathaway Christine Lee , Broshkevitch Cara Jill , Palanee-Phillips Thesla , Barnabas Ruanne Vanessa , Rao Darcy White , Sharma Monisha
Published: April 2024

Introduction

Women living with human immunodeficiency virus (WLHIV) face elevated risks of human papillomavirus (HPV) acquisition and cervical cancer (CC). Coverage of CC screening and treatment remains low in low-and-middle-income settings, reflecting resource challenges and loss to follow-up with current strategies. We estimated the health and economic impact of alternative scalable CC screening strategies in KwaZulu-Natal, South Africa, a region with high burden of CC and HIV.

Methods

We parameterized a dynamic compartmental model of HPV and HIV transmission and CC natural history to KwaZulu-Natal. Over 100 years, we simulated the status quo of a multi-visit screening and treatment strategy with cytology and colposcopy triage (South African standard of care) and six single-visit comparator scenarios with varying: 1) screening strategy (HPV DNA testing alone, with genotyping, or with automated visual evaluation triage, a new high-performance technology), 2) screening frequency (once-per-lifetime for all women, or repeated every 5 years for WLHIV and twice for women without HIV), and 3) loss to follow-up for treatment. Using the Ministry of Health perspective, we estimated costs associated with HPV vaccination, screening, and pre-cancer, CC, and HIV treatment. We quantified CC cases, deaths, and disability-adjusted life-years (DALYs) averted for each scenario. We discounted costs (2022 US dollars) and outcomes at 3% annually and calculated incremental cost-effectiveness ratios (ICERs).

Results

We projected 69,294 new CC cases and 43,950 CC-related deaths in the status quo scenario. HPV DNA testing achieved the greatest improvement in health outcomes, averting 9.4% of cases and 9.0% of deaths with one-time screening and 37.1% and 35.1%, respectively, with repeat screening. Compared to the cost of the status quo ($12.79 billion), repeat screening using HPV DNA genotyping had the greatest increase in costs. Repeat screening with HPV DNA testing was the most effective strategy below the willingness to pay threshold (ICER: $3,194/DALY averted). One-time screening with HPV DNA testing was also an efficient strategy (ICER: $1,398/DALY averted).

Conclusions

Repeat single-visit screening with HPV DNA testing was the optimal strategy simulated. Single-visit strategies with increased frequency for WLHIV may be cost-effective in KwaZulu-Natal and similar settings with high HIV and HPV prevalence.
Unraveling chronic kidney disease in children: a surprising manifestation of celiac disease
Authors: Starcea Iuliana Magdalena , Miron Ingrith , Lupu Ancuta , Ioniuc Ileana , Alecsa Mirabela , Azoicai Alice , Morariu Ionela Daniela , Munteanu Valentin , Lupu Vasile Valeriu , Mocanu Adriana
Published: April 2024

Celiac disease, firstly described in children, is a type of T-cell enteropathy that occurs in individuals genetically predisposed to gluten exposure. The estimated global prevalence of celiac disease is continuously increasing. Although, traditionally, celiac disease was diagnosed in children with failure to thrive and digestive issues, it is now recognized that may present with a wide range of symptoms beyond gastrointestinal ones. Celiac disease continues to pose significant challenges due to the continuous advancement of knowledge in understanding its pathophysiology, diagnosing the condition, managing its effects, and exploring potential therapeutic approaches. The prevalence of celiac disease is increased among individuals with chronic kidney disease, also. The most frequent associations are with diabetic nephropathy, IgA nephropathy and urolithiasis. A gut-kidney axis has been recognized to play a significant role in chronic kidney diseases. This literature review aims to review the chronic renal pathology associated with celiac disease, with emphasis on childhood.
Assessment of antimicrobial prescribing patterns, guidelines compliance, and appropriateness of antimicrobial prescribing in surgical-practice units: point prevalence survey in Malaysian teaching hospitals
Authors: Jamaluddin Nurul Adilla Hayat , Periyasamy Petrick , Lau Chee Lan , Ponnampalavanar Sasheela , Lai Pauline Siew Mei , Loong Ly Sia , Tg Abu Bakar Sidik Tg Mohd Ikhwan , Ramli Ramliza , Tan Toh Leong , Kori Najma , Yin Mei Kuen , Azman Nur Jannah , James Rodney , Thursky Karin , Naina Mohamed Isa
Published: April 2024

Objectives: This study sought to investigate the quality of antimicrobial prescribing among adult surgical inpatients besides exploring the determinants of non-compliance and inappropriate prescribing to inform stewardship activities.

Methods: A cross-sectional point prevalence study employing Hospital National Antimicrobial Prescribing Survey (Hospital NAPS) was conducted in April 2019 at two teaching hospitals in Malaysia.

Results: Among 566 surgical inpatients, 44.2% were receiving at least one antimicrobial, for a total of 339 prescriptions. Antimicrobials belonging to the World Health Organization’s Watch group were observed in 57.8% of cases. Both hospitals exhibited similar types of antimicrobial treatments prescribed and administration routes. A significant difference in antimicrobial choice was observed between hospitals (p < 0.001). Hospital with electronic prescribing demonstrated better documentation practice (p < 0.001). Guidelines compliance, 32.8% (p = 0.952) and appropriateness, 55.2% (p = 0.561) did not significantly differ. The major contributors of inappropriateness were incorrect duration, (15%) and unnecessary broad-spectrum coverage, (15.6%). Non-compliance and inappropriate prescribing were found to be 2 to 4 times significantly higher with antimicrobial prophylaxis prescription compared to empirical therapy.

Conclusion: Antimicrobial stewardship efforts to improve appropriate surgical prescribing are essential. These initiatives should prioritize surgical prophylaxis prescribing, focusing on reducing unnecessarily prolonged use and broad-spectrum antimicrobials, raising awareness among prescribers and promoting proper documentation.
Ganoderma lucidum spore extract improves sleep disturbances in a rat model of sporadic Alzheimer’s disease
Authors: Qin Yu , Zhao Yan , Hu Xiao , Chen Xi , Jiang Yan-Ping , Jin Xue-Jun , Li Gao , Li Zhen-Hao , Yang Ji-Hong , Zhang Guo-Liang , Cui Su-Ying , Zhang Yong-He
Published: April 2024

Introduction:Ganoderma lucidum (G. lucidum, Lingzhi) has long been listed as a premium tonic that can be used to improve restlessness, insomnia, and forgetfulness. We previously reported that a rat model of sporadic Alzheimer’s disease (sAD) that was induced by an intracerebroventricular injection of streptozotocin (ICV-STZ) showed significant learning and cognitive deficits and sleep disturbances. Treatment with a G. lucidum spore extract with the sporoderm removed (RGLS) prevented learning and memory impairments in sAD model rats.

Method: The present study was conducted to further elucidate the preventive action of RGLS on sleep disturbances in sAD rats by EEG analysis, immunofluorescence staining, HPLC-MS/MS and Western blot.

Results: Treatment with 720 mg/kg RGLS for 14 days significantly improved the reduction of total sleep time, rapid eye movement (REM) sleep time, and non-REM sleep time in sAD rats. The novelty recognition experiment further confirmed that RGLS prevented cognitive impairments in sAD rats. We also found that RGLS inhibited the nuclear factor-κB (NF-κB)/Nod-like receptor family pyrin domain-containing 3 (NLRP3) inflammatory pathway in the medial prefrontal cortex (mPFC) in sAD rats and ameliorated the lower activity of γ-aminobutyric acid (GABA)-ergic neurons in the parabrachial nucleus (PBN).

Discussion: These results suggest that inhibiting the neuroinflammatory response in the mPFC may be a mechanism by which RGLS improves cognitive impairment. Additionally, improvements in PBN-GABAergic activity and the suppression of neuroinflammation in the mPFC in sAD rats might be a critical pathway to explain the preventive effects of RGLS on sleep disturbances in sAD.
Glucagon-like peptide-1 receptor agonists: new strategies and therapeutic targets to treat atherosclerotic cardiovascular disease
Authors: Wang Tianyu , Ding Juncan , Cheng Xinyi , Yang Qiang , Hu Pengfei
Published: April 2024

Atherosclerotic cardiovascular disease (ASCVD) is a leading cause of cardiovascular mortality and is increasingly prevalent in our population. Glucagon-like peptide-1 receptor agonists (GLP-1RAs) can safely and effectively lower glucose levels while concurrently managing the full spectrum of ASCVD risk factors and improving patients’ long-term prognosis. Several cardiovascular outcome trials (CVOTs) have been carried out to further investigate the cardiovascular benefits of GLP-1RAs. Analyzing data from CVOTs can provide insights into the pathophysiologic mechanisms by which GLP-1RAs are linked to ASCVD and define the use of GLP-1RAs in clinical practice. Here, we discussed various mechanisms hypothesized in previous animal and preclinical human studies, including blockade of the production of adhesion molecules and inflammatory factors, induction of endothelial cells’ synthesis of nitric oxide, protection of mitochondrial function and restriction of oxidative stress, suppression of NOD-like receptor thermal protein domain associated protein three inflammasome, reduction of foam cell formation and macrophage inflammation, and amelioration of vascular smooth muscle cell dysfunction, to help explain the cardiovascular benefits of GLP-1RAs in CVOTs. This paper provides an overview of the clinical research, molecular processes, and possible therapeutic applications of GLP-1RAs in ASCVD, while also addressing current limitations in the literature and suggesting future research directions.
Sporoderm-removed Ganoderma lucidum spores ameliorated early depression-like behavior in a rat model of sporadic Alzheimer’s disease
Authors: Zhao Yan , Qin Yu , Hu Xiao , Chen Xi , Jiang Yan-Ping , Jin Xue-Jun , Li Gao , Li Zhen-Hao , Yang Ji-Hong , Cui Su-Ying , Zhang Yong-He
Published: April 2024

Introduction:Ganoderma lucidum: (G. lucidum, Lingzhi) is a medicinal and edible homologous traditional Chinese medicine that is used to treat various diseases, including Alzheimer’s disease and mood disorders. We previously reported that the sporoderm-removed G. lucidum spore extract (RGLS) prevented learning and memory impairments in a rat model of sporadic Alzheimer’s disease (sAD), but the effect of RGLS on depression-like behaviors in this model and its underlying molecular mechanisms of action remain unclear.

Method: The present study investigated protective effects of RGLS against intracerebroventricular streptozotocin (ICV-STZ)-induced depression in a rat model of sAD and its underlying mechanism. Effects of RGLS on depression- and anxiety-like behaviors in ICV-STZ rats were assessed in the forced swim test, sucrose preference test, novelty-suppressed feeding test, and open field test.

Results: Behavioral tests demonstrated that RGLS (360 and 720 mg/kg) significantly ameliorated ICV-STZ-induced depression- and anxiety-like behaviors. Immunofluorescence, Western blot and enzyme-linked immunosorbent assay results further demonstrated that ICV-STZ rats exhibited microglia activation and neuroinflammatory response in the medial prefrontal cortex (mPFC), and RGLS treatment reversed these changes, reflected by the normalization of morphological changes in microglia and the expression of NF-κB, NLRP3, ASC, caspase-1 and proinflammatory cytokines. Golgi staining revealed that treatment with RGLS increased the density of mushroom spines in neurons. This increase was associated with elevated expression of brain-derived neurotrophic protein in the mPFC.

Discussion: In a rat model of ICV-STZ-induced sAD, RGLS exhibits antidepressant-like effects, the mechanism of which may be related to suppression of the inflammatory response modulated by the NF-κB/NLRP3 pathway and enhancement of synaptic plasticity in the mPFC.
Chickpea (Cicer arietinum L.) growth, nodulation, and yield as affected by varieties, Mesorhizobium strains, and NPSB fertilizer in Southern Ethiopia
Authors: Nahusenay Gashaw , Wolde Girma , Tena Wondwosen , Tamiru Tatek
Published: April 2024

A significant legume crop in Ethiopia, chickpeas (Cicer arietinum L.) have several advantages, including high nutritional value and the capacity to improve soils deficient in nitrogen through biological nitrogen fixation using several endosymbiotic Mesorhizobium spp. strains. However, the host variety, the soil’s capacity to hold nutrients, and the endosymbiont’s innate physiological traits all affect how efficient the strains are. The primary obstacles to its cultivation in the research area are inadequate agronomic methods and low soil fertility [low nitrogen (N), low soil organic matter (OM), low accessible phosphorous (P), sulfur (S), and boron (B)], which results in ineffective nodulation. To evaluate the effects of NPSB fertilization and inoculation, a field experiment was carried out in Buchach Kebele’s Cheha area during the primary cropping season of 2021/22. The trial included two chickpea kinds (Local and Arerti), two NPSB levels (zero and 121 kg NPSB ha-1), and four levels of Mesorhizobium strains (CP-M41, CP-EAL 029, CP-M20b, and un-inoculated control). A randomized complete block design (RCBD) was used to organize the treatments in a factorial form with three replications. In comparison to the single application and the control, the interaction impact of strains, NPSB fertilizer, and variety greatly increased nodulation parameters, growth parameters, yield, and yield components. The Arerti variety combined with the CP-M41 Mesorhizobium strain and NPSB fertilizer had the maximum grain production (3177.16 kg ha-1). It yielded 15.96%, 24.06%, and 37.93% more than the Arerti with CP-M41 strain, Arerti with NPSB, and the control treatments, respectively. The partial budget analysis of the study treatments showed that the Arerti variety with the combined application of 121 kg NPSB ha-1 and Mesorhizobium strain CP-M41 inoculation produced the highest net return (102,092.6 ETB ha-1) with an acceptable marginal rate of return (618%). It has been found that the CP-M41 strain and the Arerti variety, when combined with 121 kg NPSB ha-1 application, is a suitable treatment combination to achieve increased chickpea crop yield and profit in the studied area. However, the results need further validation in the farmer’s field before recommending to farmers.
Genetic basis of local adaptation in the cold-tolerant mangrove Kandelia obovata
Authors: Zou Chuangchao , Wang Yushuai , Zhou Renchao , Tang Tian
Published: April 2024

Understanding the genetic basis of local adaption is crucial in the context of global climate change. Mangroves, as salt-tolerant trees and shrubs in the intertidal zone of tropical and subtropical coastlines, are particularly vulnerable to climate change. Kandelia obovata, the most cold-tolerant mangrove species, has undergone ecological speciation from its cold-intolerant counterpart, Kandelia candel, with geographic separation by the South China Sea. In this study, we conducted whole-genome re-sequencing of K. obovata populations along China’s southeast coast, to elucidate the genetic basis responsible for mangrove local adaptation to climate. Our analysis revealed a strong population structure among the three K. obovata populations, with complex demographic histories involving population expansion, bottleneck, and gene flow. Genome-wide scans unveiled pronounced patterns of selective sweeps in highly differentiated regions among pairwise populations, with stronger signatures observed in the northern populations compared to the southern population. Additionally, significant genotype-environment associations for temperature-related variables were identified, while no associations were detected for precipitation. A set of 39 high-confidence candidate genes underlying local adaptation of K. obovata were identified, which are distinct from genes under selection detected by comparison between K. obovata and its cold-intolerant relative K. candel. These results significantly contribute to our understanding of the genetic underpinnings of local adaptation in K. obovata and provide valuable insights into the evolutionary processes shaping the genetic diversity of mangrove populations in response to climate change.
From contact to connection: a comprehensive examination of affective touch in educational settings
Authors: El Hakim Sonia , Farsani Danyal
Published: April 2024

We often talk about the way we talk, and we frequently try to see the way we see, but for some reasons we have rarely touched on the way we touch. The communication we transmit with touch is perceived to be one of the most powerful means of establishing human relationships. In particular, tactile communication with parents, caregivers and teachers is particularly important for infants and students, as it helps make stronger relationships between educators or teachers and schoolers and also between students. Research has demonstrated the numerous benefits that an affective touch has on students, physically, socially and cognitively, or as has observed, touch touches deeper that just one’s skin and it is a recipe for creating meaningful relations. However, in the educational context, touch is perceived to be a complex phenomenon full of tension and emotion. For years, a dilemma has arisen in educational institutions in some countries, whether teachers can touch students or not? Despite the benefits that affective touch brings to students, cases of sexual abuse and inappropriate behavior at school have alerted the education system, to such an extent that many teachers worldwide consider what is and is not appropriate when communicating affectively with their students through touch. In this perspective article, by drawing on previous literature reviews, we shall highlight the benefits that affective touch has on learners.
The influence of leader-subordinate emotional intelligence congruence on the flow experience: evidence from the liquor manufacturing industry
Authors: Lu Zhuoran
Published: April 2024

Previous research has provided evidence supporting a positive correlation between emotional intelligence (EI) and flow. However, intriguing findings in the liquor manufacturing industry prompt me to consider the necessity of examining the effects of leader-subordinate EI congruence on flow. Therefore, this article begins with two questions: First, what is the impact of leader-subordinate EI congruence on flow? Second, do two distinct congruent scenarios (i.e., high-high and low-low) exhibit significant nuances that differentially influence flow? To answer these questions, this study utilizes polynomial regression and three-dimensional response surface analysis methods. A multi-source and three-phase investigation method was used to collect data from 279 subordinates and 56 leaders in the liquor manufacturing industry. Based on the person-environment (PE) fit theory, this study investigates the influence of leader-subordinate EI congruence on flow while considering leader-member exchange (LMX) as a mediator in these processes. The current study not only demonstrates a positive relationship between leader-subordinate EI congruence and LMX, but also reveals that a high-high EI matching pattern can enhance the favorable dynamics of congruence and yield higher LMX compared to a low-low matching pattern. Furthermore, this study identifies LMX as a mediator in the positive relationship between leader-subordinate EI congruence and flow. Additionally, although the incongruent scenarios are beyond the scope of this study, the findings demonstrate that a high EI subordinate paired with a low EI leader has a more detrimental impact on the LMX than a low EI subordinate paired with a high EI leader. Theoretical and practical implications are discussed.
The influence of gratitude and personality traits on career resilience and career success among college students
Authors: Zhao Kai , Ji Wenna
Published: April 2024

Introduction

The study examines the interplay between gratitude and career success, with career resilience as the mediating mechanism and personality traits, i.e., conscientiousness and extraversion, as moderating factors. The overarching goal is to enhance our understanding of the complex dynamics that influence career outcomes of college students in China.

Methods

Data are gathered through a survey-based technique, capturing responses from a diverse sample of participants. The analysis employs Structural Equation Modeling (SEM) to explore the relationships among gratitude, career resilience, personality traits, and career success.

Results

The results reveal that gratitude impacts students’ career success through the mediating mechanism of career resilience. In addition, conscientiousness and extraversion are found to positively intervene the direct effect between gratitude and career resilience and the indirect effect between gratitude and career success through career resilience.

Discussion

The findings offer valuable insights for individuals, organizations, and career development practitioners, emphasizing the importance of cultivating gratitude and recognizing the differential impact of personality traits on this process. As organizations seek to foster resilient and successful career paths, acknowledging these nuanced dynamics can inform targeted interventions and strategies.
Cognitive mechanisms and neurological foundations of companion animals’ role in enhancing human psychological well-being
Authors: Liu Heng , Lin Jingyuan , Lin Wuji
Published: April 2024

The impact of companion animals on human psychological health has garnered widespread attention. Research demonstrates that companion animals contribute positively in various ways, including reducing depression, anxiety, stress, and fostering positive emotions in humans. Recent studies have revealed significant changes in the activity levels of human emotion-related cortical areas (such as the frontal cortex and amygdala) and neurotransmitter (e.g., oxytocin, cortisol) secretion due to interaction with companion animals. However, research in this domain is still in a nascent stage, with many unknowns in the cognitive neural mechanisms involved. This paper proposes that to understand the cognitive mechanisms through which companion animals affect human psychological health, we need to examine changes in emotional cognitive processing. It aims to uncover the neurological underpinnings of how companion animals enhance human psychological well-being from the perspective of brain connectivity. This approach is expected to provide theoretical support and direction for future research and practical applications in this field.
The influence of social exclusion on prosocial behavior of college students: the role of relational need threat and regulatory focus
Authors: Hou Chunna , Li Shuyu , Shi Haoling , Liu Zhijun
Published: April 2024

The present study investigated the impact of social exclusion on prosocial behavior, examining the roles of relational need threat and regulatory focus. Utilizing a questionnaire study with 483 participants (Study 1) and an experimental study with 100 participants (Study 2), we found that (1) social exclusion negatively predicted prosocial behavior; (2) relational need threat fully mediated the relationship between social exclusion and prosocial behavior; and (3) regulatory focus, categorized as either promotion or prevention, moderated this relationship in opposite directions. In conclusion, our findings reveal that social exclusion does indeed trigger prosocial behavior. Meanwhile, relational need threat and regulatory focus have a co-action impact on this process. These findings have been carefully discussed within the frameworks of the temporal need-threat model and the cognitive-affective personality system theory.
Differential effect of China’s Zero Markup Drug Policy on provider-induced demand in secondary and tertiary hospitals
Authors: Zhang Xiaoxi , Zimmerman Armand , Lai Hongyu , Zhang Yanyan , Tang Zhongyi , Tang Shenglan , Ogbuoji Osondu
Published: April 2024

Following the marketization of China’s health system in the 1980’s, the government allowed public hospitals to markup the price of certain medications by 15% to compensate for reduced revenue from government subsidies. This incentivized clinicians to induce patient demand for drugs which resulted in higher patient out-of-pocket payments, higher overall medical expenditure, and poor health outcomes. In 2009, China introduced the Zero Markup Drug Policy (ZMDP) which eliminated the 15% markup. Using Shanghai as a case study, this paper analyzes emerging and existing evidence about the impact of ZMDP on hospital expenditure and revenue across secondary and tertiary public hospitals. We use data from 150 public hospitals across Shanghai to examine changes in hospital expenditure and revenue for various health services following the implementation of ZMDP. Our analysis suggests that, across both secondary and tertiary hospitals, the implementation of ZMDP reduced expenditure on drugs but increased expenditure on medical services, exams, and tests thereby increasing hospital revenue and keeping inpatient and outpatient costs unchanged. Moreover, our analysis suggests that tertiary facilities increased their revenue at a faster rate than secondary facilities, likely due to their ability to prescribe more advanced and, therefore, more costly procedures. While rigorous experimental designs are needed to confirm these findings, it appears that ZMDP has not reduced instances of medical expenditure provoked by provider-induced demand (PID) but rather shifted the effect of PID from one revenue source to another with differential effects in secondary vs. tertiary hospitals. Supplemental policies are likely needed to address PID and reduce patient costs.
Dose–response associations between physical activity and sedentary time with functional disability in older adults with or without frailty: a prospective cohort study
Authors: Seino Satoshi , Abe Takumi , Nofuji Yu , Hata Toshiki , Shinkai Shoji , Kitamura Akihiko , Fujiwara Yoshinori
Published: April 2024

Purpose

Evidence regarding the dose–response curve shapes of physical activity (PA) and sedentary time (ST) in older adults with functional disability (FD) is extremely limited. Moreover, these associations may differ depending on with/without frailty. We examined the dose–response associations between moderate-to-vigorous PA (MVPA) and ST with FD among older adults with/without frailty.

Methods

We included 7,480 initially nondisabled adults (3,795 men and 3,685 women) aged 65–84 years in Ota City, Tokyo, Japan. MVPA and ST were evaluated using the International Physical Activity Questionnaire-Short Form. FD was prospectively identified using a nationally unified database of the long-term care insurance system. Frailty was determined using Check-List 15, validated against Fried’s frailty criteria. Multivariate-adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) of MVPA and ST for FD were calculated, and dose–response curves were examined using restricted cubic splines.

Results

During 3.6 years of follow-up, 1,001 (13.4%) participants had FD. Among all participants, compared with no MVPA, the HRs for FD reduced linearly up to approximately 2000 metabolic equivalents (METs)■min/week of MVPA, and the lowest HR (HR: 0.61, 95% CI: 0.51–0.74) was reached at around 3,000–4,000 METs■min/week. Although the shape of this association was consistent regardless of with/without frailty, the magnitude of the association tended to be stronger in frail older adults than in non-frail older adults. Compared with those for the median (300 min/day) of ST, the HRs for FD increased linearly as ST reached approximately 600 min/day or more, independent of MVPA, with a maximum HR of 1.31 (95% CI: 1.01–1.71) for 1,080 min/day among all participants. This association was more pronounced among non-frail older adults but not statistically significant among frail older adults.

Conclusion

Higher MVPA levels consistently reduced the incidence of FD regardless of frailty in a significant inverse nonlinear dose–response manner. A significant positive nonlinear dose–response association between ST and FD risk was identified among non-frail older adults but not among frail older adults. Increasing MVPA and reducing prolonged ST are important for preventing FD among non-frail older adults. However, reducing ST alone may be insufficient; increasing MVPA, even if by only small increments, is highly recommended for frail older adults.
“I am still human and worth a life:” a qualitative study of the impacts of a community based, peer-led, treatment support model for young adults living with HIV in Zimbabwe
Authors: Zamudio-Haas Sophia , Mahaka Imelda , Chapwanya Gwendoline , Dunbar Megan S. , Lightfoot Marguerita
Published: April 2024

Background

A persistent treatment gap remains between children and adults living with HIV. The Zvandiri program, developed by Africaid, is one of the few models of differentiated service delivery for children, adolescents, and youth that has been shown to improve outcomes along the HIV care continuum, employing Community Adolescent Treatment Supporters (CATS) to offer peer counseling and patient navigation. Our qualitative study provides an in-depth analysis of the feelings and experiences Zimbabwean youth had following an HIV diagnosis, and the ways that CATS facilitated linkage and retention in care.

Methods

We conducted in-depth interviews in Shona with adolescents and young adults who were recently diagnosed with HIV in Zimbabwe. Interviews were audio-recorded, transcribed, and then translated to English. Interviews were coded in Dedoose using a structured a priori codebook. We wrote semi-structured summary memos for each interview. We co-conducted thematic analysis, guided by interpretive phenomenology with a team of Zimbabwean and American experienced qualitative researchers and community partners. We co-developed memos to elaborate and understand key themes across interviews.

Results

Most of our interview participants recounted an immediate sense of loss upon testing HIV positive and a fear that “there was no hope for the future.” CATS played a pivotal role for youth, providing emotional, educational, and logistical support to facilitate treatment initiation, adherence, and persistence in care. The CATS program supported youth through multiple approaches: group sessions, individual meetings, and via text or phone. While CATS offered counseling and comfort to participants, they emphasized the long-term importance of identifying at least one other person in participants’ lives who could know their status and support them around HIV.

Conclusion

Our findings delineate some of the key concerns that face youth after receiving an HIV diagnosis and the ways that a community-based adherence peer navigation program supported participants to navigate both their feelings and the health care system. Results can inform practice at community-based agencies that are implementing or considering peer youth navigation programs and garner support for policy to fund interventions for youth.
Examining students’ level of understanding toward COVID-19 and its associated factors in Ethiopia: a systematic review and meta-analysis
Authors: Chereka Alex Ayenew , Demsash Addisalem Workie , Butta Fikadu Wake , Shibabaw Adamu Ambachew , Gebeyehu Chernet Desalegn , Adane Daniel , Limeneh Wubalem Dilie , Fentahun Amare Dagnew , Tadesse Mathias Nega , Kitil Gemeda Wakgari
Published: April 2024

Background

This systematic review and meta-analysis aim to investigate students’ understanding of COVID-19 in Ethiopia and identify associated factors. The primary goal is to pinpoint key contributors shaping students’ perception and comprehension of the virus. The study aims to offer valuable insights for developing targeted educational interventions, ultimately enhancing students’ overall knowledge and awareness of the pandemic in the specific context of Ethiopia.

Methods

The study adhered rigorously to PRISMA criteria, ensuring a standardized methodology. Data from reputable databases like Google Scholar and PubMed were systematically collected. Ten relevant articles were meticulously analyzed using STATA version 11, with heterogeneity assessed by the I2 test. A funnel plot and Egger’s test were used to check for publication bias. The determination of the pooled effect size utilized a random-effect model meta-analysis, offering a robust 95% confidence interval.

Results

This meta-analysis, based on 10 articles, reveals an overall prevalence of 61.58% (95% CI: 47.26–75.89). Significant contributors to students’ comprehension include Social media users (AOR) = 2.38, urban residence AOR = 3.31, news media followers AOR = 2.51, fathers’ educational status AOR = 2.35, watching television AOR = 4.71, and health science students AOR = 4.21. These findings underscore crucial elements shaping students’ understanding of COVID-19 in Ethiopia.

Conclusion

Our analysis indicates that 61.58% of Ethiopian students possess a good understanding of COVID-19. Factors such as active social media engagement, geographic location, frequency of news consumption, father’s level of education, television viewing habits, and enrollment in health science programs significantly influence their comprehension. These findings underscore the importance of implementing targeted interventions to enhance health literacy and education among students, thereby facilitating a more effective response to pandemics.