bluuebunny/crossref_metadata_2025 huggingface dataset:
A New and Direct R-Value Measurement Method of Sheet Metal Based on Multi-Camera DIC System
Authors: Fang Siyuan , Zheng Xiaowan , Zheng Gang , Zhang Boyang , Guo Bicheng , Yang Lianxiang
Published: September 2021

More and more attention has been given in the field of mechanical engineering to a material’s R-value, a parameter that characterizes the ability of sheet metal to resist thickness strain. Conventional methods used to determine R-value are based on experiments and an assumption of constant volume. Because the thickness strain cannot be directly measured, the R-value is currently determined using experimentally measured strains in the width, and loading directions in combination with the constant volume assumption, to determine the thickness strain indirectly. This paper provides an alternative method for determining the R-value without any assumptions. This method is based on the use of a multi-camera DIC system to measure strains in three directions simultaneously. Two sets of stereo-vision DIC measurement systems, each comprised of two GigE cameras, are placed on the front and back sides of the sample. Use of the double-sided calibration strategy unifies the world coordinate system of the front and back DIC measurement systems to one coordinate system, allowing for the measurement of thickness strain and direct calculation of R-value. The Random Sample Consensus (RANSAC) algorithm is used to eliminate noise in the thickness strain data, resulting in a more accurate R-value measurement.
Fracture Estimation in Ship Collision Analysis—Strain Rate and Thermal Softening Effects
Authors: Cerik Burak Can , Choung Joonmo
Published: September 2021

This study examined the effects of the strain rate and thermal softening on large-scale ductile fracture in ship collisions using a rate-dependent combined localized necking and fracture model. A Johnson–Cook type-hardening model, consisting of strain hardening, rate-sensitivity, and thermal softening terms, was adopted together with an associated flow rule. The temperature was treated as an internal state variable and was calculated from the plastic strain energy using a strain-rate-dependent weighting function under fully isothermal and adiabatic conditions. At every time increment, the fracture locus was updated based on the temporal strain rate, whereas the necking locus was coupled with the hardening law, which was dependent on both the strain rate and temperature. The damage indicator framework was used to consider the non-proportional loading paths. The dynamic shell-element failure model was verified through plate-panel penetration tests and applied to a large-scale ship collision analysis involving a struck ship/ship-shaped offshore installation and a supply vessel. The effects of the loading rate and impact energy were assessed in terms of the global behavior of the structure and observed failure modes.
A Role for Inositol Pyrophosphates in the Metabolic Adaptations to Low Phosphate in Arabidopsis
Authors: Land Eric S. , Cridland Caitlin A. , Craige Branch , Dye Anna , Hildreth Sherry B. , Helm Rich F. , Gillaspy Glenda E. , Perera Imara Y.
Published: September 2021

Phosphate is a major plant macronutrient and low phosphate availability severely limits global crop productivity. In Arabidopsis, a key regulator of the transcriptional response to low phosphate, phosphate starvation response 1 (PHR1), is modulated by a class of signaling molecules called inositol pyrophosphates (PP-InsPs). Two closely related diphosphoinositol pentakisphosphate enzymes (AtVIP1 and AtVIP2) are responsible for the synthesis and turnover of InsP8, the most implicated molecule. This study is focused on characterizing Arabidopsis vip1/vip2 double mutants and their response to low phosphate. We present evidence that both local and systemic responses to phosphate limitation are dampened in the vip1/vip2 mutants as compared to wild-type plants. Specifically, we demonstrate that under Pi-limiting conditions, the vip1/vip2 mutants have shorter root hairs and lateral roots, less accumulation of anthocyanin and less accumulation of sulfolipids and galactolipids. However, phosphate starvation response (PSR) gene expression is unaffected. Interestingly, many of these phenotypes are opposite to those exhibited by other mutants with defects in the PP-InsP synthesis pathway. Our results provide insight on the nexus between inositol phosphates and pyrophosphates involved in complex regulatory mechanisms underpinning phosphate homeostasis in plants.
Stimulation of Toll-Like Receptor 3 Diminishes Intracellular Growth of Salmonella Typhimurium by Enhancing Autophagy in Murine Macrophages
Authors: Lee Hyo-Ji , Lee Sun-Hye , Jeon Ji-Hui , Kim Hyo-Jung , Jeong Eui-Kwon , Kim Min-Jeong , Jung Young Mee , Jung Yu-Jin
Published: September 2021

The Salmonella enterica serovar Typhimurium (S. Typhimurium) is a facultative Gram-negative bacterium that causes acute gastroenteritis and food poisoning. S. Typhimurium can survive within macrophages that are able to initiate the innate immune response after recognizing bacteria via various pattern-recognition receptors (PRRs), such as Toll-like receptors (TLRs). In this study, we investigated the effects and molecular mechanisms by which agonists of endosomal TLRs—especially TLR3—contribute to controlling S. Typhimurium infection in murine macrophages. Treatment with polyinosinic:polycytidylic acid (poly(I:C))—an agonist of TLR3—significantly suppressed intracellular bacterial growth by promoting intracellular ROS production in S. Typhimurium-infected cells. Pretreatment with diphenyleneiodonium (DPI)—an NADPH oxidase inhibitor—reduced phosphorylated MEK1/2 levels and restored intracellular bacterial growth in poly(I:C)-treated cells during S. Typhimurium infection. Nitric oxide (NO) production increased through the NF-κB-mediated signaling pathway in poly(I:C)-treated cells during S. Typhimurium infection. Intracellular microtubule-associated protein 1A/1B-light chain 3 (LC3) levels were increased in poly(I:C)-treated cells; however, they were decreased in cells pretreated with 3-methyladenine (3-MA)—a commonly used inhibitor of autophagy. These results suggest that poly(I:C) induces autophagy and enhances ROS production via MEK1/2-mediated signaling to suppress intracellular bacterial growth in S. Typhimurium-infected murine macrophages, and that a TLR3 agonist could be developed as an immune enhancer to protect against S. Typhimurium infection.
The Role of Sch9 and the V-ATPase in the Adaptation Response to Acetic Acid and the Consequences for Growth and Chronological Lifespan
Authors: Deprez Marie-Anne , Maertens Jeroen M. , Olsson Lisbeth , Bettiga Maurizio , Winderickx Joris
Published: September 2021

Studies with Saccharomyces cerevisiae indicated that non-physiologically high levels of acetic acid promote cellular acidification, chronological aging, and programmed cell death. In the current study, we compared the cellular lipid composition, acetic acid uptake, intracellular pH, growth, and chronological lifespan of wild-type cells and mutants lacking the protein kinase Sch9 and/or a functional V-ATPase when grown in medium supplemented with different acetic acid concentrations. Our data show that strains lacking the V-ATPase are especially more susceptible to growth arrest in the presence of high acetic acid concentrations, which is due to a slower adaptation to the acid stress. These V-ATPase mutants also displayed changes in lipid homeostasis, including alterations in their membrane lipid composition that influences the acetic acid diffusion rate and changes in sphingolipid metabolism and the sphingolipid rheostat, which is known to regulate stress tolerance and longevity of yeast cells. However, we provide evidence that the supplementation of 20 mM acetic acid has a cytoprotective and presumable hormesis effect that extends the longevity of all strains tested, including the V-ATPase compromised mutants. We also demonstrate that the long-lived sch9Δ strain itself secretes significant amounts of acetic acid during stationary phase, which in addition to its enhanced accumulation of storage lipids may underlie its increased lifespan.
Epidemiological Surveillance of Lyme Borreliosis in Bavaria, Germany, 2013–2020
Authors: Böhmer Merle Margarete , Ens Katharina , Böhm Stefanie , Heinzinger Susanne , Fingerle Volker
Published: September 2021

Lyme borreliosis (LB) is the most common tick-borne disease in Germany. Mandatory notification of acute LB manifestations (erythema migrans (EM), neuroborreliosis (NB), and Lyme arthritis (LA)) was implemented in Bavaria on 1 March 2013. We aimed to describe the epidemiological situation and to identify LB risk areas and populations. Therefore, we analyzed LB cases notified from March 2013 to December 2020 and calculated incidence (cases/100,000 inhabitants) by time, place, and person. Overall, 35,458 cases were reported during the study period (EM: 96.7%; NB: 1.7%; LA: 1.8%). The average incidence was 34.3/100,000, but annual incidence varied substantially (2015: 23.2; 2020: 47.4). Marked regional differences at the district level were observed (annual average incidence range: 4–154/100,000). The Bavarian Forest and parts of Franconia were identified as high-risk regions. Additionally, high risk for LB was found in 5–9-year-old males and in 60–69-year-old females. The first group also had the highest risk of a severe disease course. We were able to identify areas and populations in Bavaria with an increased LB risk, thereby providing a basis for targeted measures to prevent LB. Since LB vaccination is currently not available, such measures should comprise (i) avoiding tick bites, (ii) removing ticks rapidly after a bite, and (iii) treating LB early/adequately.
Iron-Speciation Control of Chalcopyrite Dissolution from a Carbonatite Derived Concentrate with Acidic Ferric Sulphate Media
Authors: Nyembwe Kolela J. , Fosso-Kankeu Elvis , Waanders Frans , Mkandawire Martin
Published: September 2021

The mechanisms involved in the dissolution of chalcopyrite from a carbonatite concentrate in a ferric sulphate solution at pH 1.0, 1.5 and 1.8, and temperatures 25 °C and 50 °C were investigated. Contrary to expectations and thermodynamic predictions according to which low pH would favour high Cu dissolution, the opposite was observed. The dissolution was also highly correlated to the temperature. CuFeS2 phase dissolution produced intermediate Cu rich phases: CuS, Cu2S and Cu5FeS4, which appeared to envelop CuFeS2. Thermodynamic prediction revealed CuS to be refractory and could hinder dissolution. CuFeS2 phase solid-state dissolution process was further discussed. Free Fe3+ and its complexes (Fe(HSO4)2+, Fe(SO4)2– and FeSO4+ were responsible for Cu dissolution, which increased with increasing pH and temperature. The dissolution improved at pH 1.8 rather than 1.0 due to the increase of (Fe(HSO4)2+, Fe(SO4)2– and FeSO4+, which were also the predominating species at a higher temperature. The fast and linear first dissolution stage was attributed to the combined effect of Fe3+ and its complex (Fe(HSO4)2+, while Fe(SO4)2– was the main species for the second Cu dissolution stage characterised by a slow rate.
Photo-Switchable Aggregation-Induced Emission of Bisthienylethene-Dipyrimido[2,1-b][1,3]benzothiazole Triad
Authors: Gong Shan-Shan , Zheng Chun-Hong , Chen Zhen-Zhen , Yang Dong-Zhao , Chi Mei , Pu Shou-Zhi , Sun Qi
Published: September 2021

A bisthienylethene-dipyrimido[2,1-b][1,3]benzothiazole (BTE-2PBT) triad has been designed and synthesized based on our recent discovery of PBTs as atypical propeller-shaped novel AIEgens. The triad not only maintains the photochromic properties of BTE moiety in solution, film, and solid state but also exhibits remarkable AIE properties. Moreover, the fluorescence of BTE-2PBT PMMA film could be modulated with high contrast by alternate UV and visible light irradiation. Photoerasing, rewriting, and non-destructive readout of fluorescent images on BTE-2PBT PMMA film well demonstrate its potential application as optical memory media.
In Vitro Evaluation of Antibacterial and Antifungal Activity of Biogenic Silver and Copper Nanoparticles: The First Report of Applying Biogenic Nanoparticles against Pilidium concavum and Pestalotia sp. Fungi
Authors: Bayat Maryam , Zargar Meisam , Chudinova Elena , Astarkhanova Tamara , Pakina Elena
Published: September 2021

There is increased attention paid to metallic nanoparticles due to their intensive use in various branches of agriculture and biotechnology, such as pest management, nanosensors, gene delivery, seed treatment, etc. There has been growing interest in applying environmentally friendly strategies for synthesizing nanoparticles without using substances which are hazardous to the environment. Biological practices for the synthesis of nanoparticles have been considered as possible ecofriendly alternatives to chemical synthesis. In the present study, we used biogenic silver and copper nanoparticles which were prepared by a previously reported green method. Moreover, the problem of chemical residues, which usually remain along with chemically synthesized nanoparticles and limit their application, was solved by developing such a green synthesis approach. To study the antibacterial activity of silver and copper nanoparticles, Pseudomonas aeruginosa was used; for the evaluation of antifungal activity, the pathogenic fungi Botrytis cinerea, Pilidium concavum and Pestalotia sp. were applied. To the best of our knowledge, this study represents the first time that the antifungal impact of a nanoparticle has been tested on Pilidium concavum and Pestalotia sp. Silver nanoparticles were found to be the more effective antimicrobial agent against all examined pathogens in comparison to copper nanoparticles. Data from such investigations provide valuable preliminary data on silver nanoparticle-based compounds or composites for use in the management of different pathogens.
The Structural and Dynamical Properties of the Hydration of SNase Based on a Molecular Dynamics Simulation
Authors: Liu Hangxin , Xiang Shuqing , Zhu Haomiao , Li Li
Published: September 2021

The dynamics of protein–water fluctuations are of biological significance. Molecular dynamics simulations were performed in order to explore the hydration dynamics of staphylococcal nuclease (SNase) at different temperatures and mutation levels. A dynamical transition in hydration water (at ~210 K) can trigger larger-amplitude fluctuations of protein. The protein–water hydrogen bonds lost about 40% in the total change from 150 K to 210 K, while the Mean Square Displacement increased by little. The protein was activated when the hydration water in local had a comparable trend in making hydrogen bonds with protein– and other waters. The mutations changed the local chemical properties and the hydration exhibited a biphasic distribution, with two time scales. Hydrogen bonding relaxation governed the local protein fluctuations on the picosecond time scale, with the fastest time (24.9 ps) at the hydrophobic site and slowest time (40.4 ps) in the charged environment. The protein dynamic was related to the water’s translational diffusion via the relaxation of the protein–water’s H-bonding. The structural and dynamical properties of protein–water at the molecular level are fundamental to the physiological and functional mechanisms of SNase.
Active Plasmonics with Responsive, Binary Assemblies of Gold Nanorods and Nanospheres
Authors: Szustakiewicz Piotr , Kowalska Natalia , Bagiński Maciej , Lewandowski Wiktor
Published: September 2021

Self-assembly of metal nanoparticles has applications in the fabrication of optically active materials. Here, we introduce a facile strategy for the fabrication of films of binary nanoparticle assemblies. Dynamic control over the configuration of gold nanorods and nanospheres is achieved via the melting of bound and unbound fractions of liquid-crystal-like nanoparticle ligands. This approach provides a route for the preparation of hierarchical nanoparticle superstructures with applications in reversibly switchable, visible-range plasmonic technologies.
Bioinspired Microstructured Polymer Surfaces with Antireflective Properties
Authors: Wetzel Alexandre Emmanuel , del Castillo Iniesta Nuria , Engay Einstom , Mandsberg Nikolaj Kofoed , Schou Dinesen Celine , Hanif Bilal Rashid , Berg-Sørensen Kirstine , Bunea Ada-Ioana , Taboryski Rafael
Published: September 2021

Over the years, different approaches to obtaining antireflective surfaces have been explored, such as using index-matching, interference, or micro- and nanostructures. Structural super black colors are ubiquitous in nature, and biomimicry thus constitutes an interesting way to develop antireflective surfaces. Moth-eye nanostructures, for example, are well known and have been successfully replicated using micro- and nanofabrication. However, other animal species, such as birds of paradise and peacock spiders, have evolved to display larger structures with antireflective features. In peacock spiders, the antireflective properties of their super black patches arise from relatively simple microstructures with lens-like shapes organized in tightly packed hexagonal arrays, which makes them a good candidate for cheap mass replication techniques. In this paper, we present the fabrication and characterization of antireflective microarrays inspired by the peacock spider’s super black structures encountered in nature. Firstly, different microarrays 3D models are generated from a surface equation. Secondly, the arrays are fabricated in a polyacrylate resin by super-resolution 3D printing using two-photon polymerization. Thirdly, the resulting structures are inspected using a scanning electron microscope. Finally, the reflectance and transmittance of the printed structures are characterized at normal incidence with a dedicated optical setup. The bioinspired microlens arrays display excellent antireflective properties, with a measured reflectance as low as 0.042 ± 0.004% for normal incidence, a wavelength of 550 nm, and a collection angle of 14.5°. These values were obtained using a tightly-packed array of slightly pyramidal lenses with a radius of 5 µm and a height of 10 µm.
Surface Treatment of Industrial-Grade Magnetite Particles for Enhanced Thermal Stability and Mitigating Paint Contaminants
Authors: Sinhababu Mohua , Roy Anurag , Kumar Narendra , Dutta Monojit , Sundaram Senthilarasu , Karazhanov Smagul , Udayabhanu Gopalkrishnan
Published: September 2021

Pigments can retain their color for many centuries and can withstand the effects of light and weather. The paint industry suffers from issues like aggressive moisture, corrosion, and further environmental contamination of the pigment materials. Low-cost, long-lasting, and large-scale pigments are highly desirable to protect against the challenges of contamination that exist in the paint industry. This exploratory study reinforces the color and thermal stability of industrial-grade (IG) magnetite (Fe3O4). IG Fe3O4 pigments were further considered for surface treatment with sodium hexametaphosphate (SHMP). This metaphosphate hexamer sequestrant provides good dispersion ability and a high surface energy giving thermal and dust protection to the pigment. Various physicochemical characterizations were employed to understand the effectiveness of this treatment across various temperatures (180–300 °C). The X-ray diffraction, Raman, and X-ray photoelectron spectroscopy techniques signify that the SHMP-treated Fe3O4 acquired magnetite phase stability up to 300 °C. In addition, the delta-E color difference method was also adopted to measure the effective pigment properties, where the delta-E value significantly decreased from 8.77 to 0.84 once treated with SHMP at 300 °C. The distinct color retention at 300 °C and the improved dispersion properties of surface-treated Fe3O4 positions this pigment as a robust candidate for high-temperature paint and coating applications. This study further encompasses an effort to design low-cost, large-scale, and thermally stable pigments that can protect against UV-rays, dust, corrosion, and other color contaminants that are endured by building paints.
Expansion of Preventive Dental Service Coverage for Certain Medicaid Beneficiaries in Texas: A Call for Dental Policy Effectiveness Action
Authors: Patel Naiya , Patel Neel
Published: September 2021

Background: It is vital to provide evidence-based research documentation to guide policy decision-making. There is a limited number of studies that participate in dental policy evidence-based research. Case Description: Texas is one of the states with limited dental service coverage for its Medicaid Beneficiaries. The recent senate bill 87R 1152 proposes expansion of dental preventive service to disability status Medicaid Beneficiaries. It is vital to understand how effective the extensive dental service under Medicaid coverage is through evidence-based research. True causal analysis of such public policies by utilizing observational data is only feasible through limited identification strategies. The current paper identifies that. Practical Implication: Through the identified research plan and conceptual framework, it can be established if extensive Medicaid dental service coverages effectively prevent dental disease burden in Texas.
New Insights into the Methylation of Mycobacterium tuberculosis Heparin Binding Hemagglutinin Adhesin Expressed in Rhodococcus erythropolis
Authors: Parada Cristina , Neri-Badillo Isabel Cecilia , Vallecillo Antonio J. , Segura Erika , Silva-Miranda Mayra , Guzmán-Gutiérrez Silvia Laura , Ortega Paola A. , Coronado-Aceves Enrique Wenceslao , Cancino-Villeda Laura , Torres-Larios Alfredo , Aceves Sánchez Michel de Jesús , Flores Valdez Mario Alberto , Espitia Clara
Published: September 2021

In recent years, knowledge of the role that protein methylation is playing on the physiopathogenesis of bacteria has grown. In Mycobacterium tuberculosis, methylation of the heparin binding hemagglutinin adhesin modulates the immune response, making this protein a subunit vaccine candidate. Through its C-terminal lysine-rich domain, this surface antigen interacts with heparan sulfate proteoglycans present in non-phagocytic cells, leading to extrapulmonary dissemination of the pathogen. In this study, the adhesin was expressed as a recombinant methylated protein in Rhodococcus erythropolis L88 and it was found associated to lipid droplets when bacteria were grown under nitrogen limitation. In order to delve into the role methylation could have in host–pathogen interactions, a comparative analysis was carried out between methylated and unmethylated protein produced in Escherichia coli. We found that methylation had an impact on lowering protein isoelectric point, but no differences between the proteins were found in their capacity to interact with heparin and A549 epithelial cells. An important finding was that HbhA is a Fatty Acid Binding Protein and differences in the conformational stability of the protein in complex with the fatty acid were observed between methylated and unmethylated protein. Together, these results suggest that the described role for this mycobacteria protein in lipid bodies formation could be related to its capacity to transport fatty acids. Obtained results also provide new clues about the role HbhA methylation could have in tuberculosis and point out the importance of having heterologous expression systems to obtain modified proteins.
Is Chlamydia to Blame for Koala Reproductive Cysts?
Authors: Phillips Samuel , Timms Peter , Jelocnik Martina
Published: September 2021

A significant threat to koala populations is infection from Chlamydia, which results in disease and death. Wild koalas with Chlamydia infections are admitted to wildlife hospitals and treated with antibiotics; however, up to 50% of koalas that present to wildlife hospitals do not survive. A major contributor to high mortality is the development of reproductive cysts, resulting in female infertility and euthanasia. However, the diagnosis of reproductive disease is limited to ultrasound with no further investigations. This communication highlights reports of histological and microbiological findings, the accuracy of ultrasound to necropsy reports and other possible causes for reproductive cyst development previously reported in other hosts. Our conclusions identify a significant knowledge gap in the aetiology of koala reproductive cysts and highlight the urgent need for future investigations.
Susceptibility to Clotrimazole of Candida spp. Isolated from the Genitourinary System—A Single Center Study
Authors: Frej-Mądrzak Magdalena , Golec Sabina , Włodarczyk Katarzyna , Choroszy-Król Irena , Nawrot Urszula
Published: September 2021

The aim of this study was to determine the susceptibility to clotrimazole of 125 isolates of Candida spp. originated from the genitourinary system of hospitalized patients as well as outpatients, tested in the mycological laboratory of Wroclaw Medical University in the years 1999–2018. The minimal inhibitory concentrations of clotrimazole and fluconazole were determined with the use of the microdilution method according to EUCAST, and the MFC was determined by subsequent subculture on Sabouraud agar. For the tested population of Candida yeasts, the MIC values of clotrimazole ranged from 0.008 to 8 mg/L, and MIC90 was 1 mg/L, whereas MIC50 was 0.008 mg/L. The minimal fungicidal concentration ranged between 1 and >8 mg/L. The great majority of the isolates (88%; 110/125) displayed MIC < 1 mg/L and were classified as WT (wild-type), whereas MIC ≥ 1 mg/L was determined for 2/61 (3.2%) isolates of C. albicans, 9/38 (23.6%) of C. glabrata, 1/2 of C. tropicalis, and 3/3 of C. guilliermondii. Six isolates (four of C. glabrata and two of C. albicans), defined as non-WT for clotrimazole, were classified as resistant to fluconazole, according to CBP from EUCAST. The isolates with elevated MIC to clotrimazole originated mostly from patients of the pediatric hematology unit, and their proportion in this population amounted to 17.8% (13 out of 73 isolates). In contrast, among strains from ambulatory patients, the highest observed MIC value was 1 mg/L (1 out of 37 isolates; 2.7%). The data obtained correlate well with those of most published studies on the in vitro susceptibility of Candida spp. to clotrimazole, which is usually very high. However, the existence of reports regarding the growing prevalence of resistant isolates has also to be noted. These results support the need for routinely checking the susceptibility of Candida clinical isolates to this imidazole derivative.
Applying Biotechnology in the Propagation and Further Selection of Vaccinium uliginosum × (V. corymbosum × V. angustifolium) Hybrids
Authors: Erst Anna A. , Gorbunov Aleksey B. , Asbaganov Sergey V. , Tomoshevich Maria A. , Banaev Evgeny V. , Erst Andrey S.
Published: September 2021

The most serious problem of intergeneric and interspecific hybridization is related to overcoming the reproductive isolation of different species. We assessed the efficiency of reproduction under in vitro conditions and the ex vitro growth capacity of interspecific hybrids of Vaccinium uliginosum × (V. corymbosum × V. angustifolium). The percentage of seed germination in in vitro culture was 88% for V. uliginosum, form No. 8 × (V. corymbosum × V. angustifolium), SC5-8, while it was 42% for V. uliginosum, form No. 8 × (V. corymbosum × V. angustifolium), ‘Northcountry’. The analysis of mean value showed that the multiplication rate increased and the shoot height decreased as the 2-isopentenyl adenine (2iP) concentration was increased in the nutrient medium of the studied hybrids. The maximum rate was achieved using 15 μM 2iP. A detailed analysis of the hybrids indicated that the hybrid variant reliably affected growth and development indicators. Inter simple sequence repeat analysis demonstrated that all analyzed hybrids inherited DNA fragments of the parent plants in various combinations, confirming their hybrid nature. Thus, the use of in vitro methods for the propagation and further selection of genotypes is demonstrated as being an effective approach for developing interspecific hybrids of V. uliginosum × (V. corymbosum × V. angustifolium).
Potential of the Red Alga Dixoniella grisea for the Production of Additives for Lubricants
Authors: Gavalás-Olea Antonio , Siol Antje , Sakka Yvonne , Köser Jan , Nentwig Nina , Hauser Thomas , Filser Juliane , Thöming Jorg , Lang Imke
Published: September 2021

There is an increasing interest in algae-based raw materials for medical, cosmetic or nutraceutical applications. Additionally, the high diversity of physicochemical properties of the different algal metabolites proposes these substances from microalgae as possible additives in the chemical industry. Among the wide range of natural products from red microalgae, research has mainly focused on extracellular polymers for additive use, while this study also considers the cellular components. The aim of the present study is to analytically characterize the extra- and intracellular molecular composition from the red microalga Dixoniella grisea and to evaluate its potential for being used in the tribological industry. D. grisea samples, fractionated into extracellular polymers (EPS), cells and medium, were examined for their molecular composition. This alga produces a highly viscous polymer, mainly composed of polysaccharides and proteins, being secreted into the culture medium. The EPS and biomass significantly differed in their molecular composition, indicating that they might be used for different bio-additive products. We also show that polysaccharides and proteins were the major chemical compounds in EPS, whereas the content of lipids depended on the separation protocol and the resulting product. Still, they did not represent a major group and were thus classified as a potential valuable side-product. Lyophilized algal fractions obtained from D. grisea were found to be not toxic when EPS were not included. Upon implementation of EPS as a commercial product, further assessment on the environmental toxicity to enchytraeids and other soil organisms is required. Our results provide a possible direction for developing a process to gain an environmentally friendly bio-additive for application in the tribological industry based on a biorefinery approach.
Electrochemical Reduction of CO2 to CO on Hydrophobic Zn Foam Rod in a Microchannel Electrochemical Reactor
Authors: Zhang Chunxiao , Yan Shenglin , Lin Jing , Hu Qing , Zhong Juhua , Zhang Bo , Cheng Zhenmin
Published: September 2021

Due to CO2 mass transfer limitation as well as the competition of hydrogen evolution reaction in electroreduction of CO2 in the aqueous electrolyte, Zn-based electrodes normally exhibit unsatisfying selectivity for CO production, especially at high potentials. In this work, we introduced a zinc myristate (Zn [CH3(CH2)12COO]2) hydrophobic layer on the surface of zinc foam electrode by an electrodeposition method. The obtained hydrophobic zinc foam electrode showed a high Faradaic efficiency (FE) of 91.8% for CO at −1.9 V (vs. saturated calomel electrode, SCE), which was a remarkable improvement over zinc foam (FECO = 81.87%) at the same potentials. The high roughness of the hydrophobic layer has greatly increased the active surface area and CO2 mass transfer performance by providing abundant gas-liquid-solid contacting area. This work shows adding a hydrophobic layer on the surface of the catalyst is an effective way to improve the electrochemical CO2 reduction performance.
Low-Dose Radiation Therapy for COVID-19: A Systematic Review
Authors: Mortazavi Seyed Mohammad Javad , Shams Seyedeh Fatemeh , Mohammadi Sahar , Mortazavi Seyed ALi Reza , Sihver Lembit
Published: September 2021

The ongoing COVID-19 pandemic is of great concern for the whole world, and finding an effective treatment for the disease caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is, therefore, a global race. In particular, treatment options for elderly patients and patients with genetic risk factors with COVID-19-associated pneumonia are limited, and many patients die. Low-dose radiotherapy (LDRT) of lungs was used to treat pneumonia many decades ago. Since the first report on the potential efficacy of LDRT for COVID-19-associated pneumonia was published on 1 April, 2020, tens of papers have addressed the importance of this treatment. Moreover, the findings of less than 10 clinical trials conducted to date are now available. We performed a detailed search of PubMed/MEDLINE, Web of Science, Google Scholar, and Scopus and selected the nine most relevant articles. A review of these articles was conducted. The available data indicate that in oxygen-dependent elderly patients with COVID-19-associated pneumonia, whole-lung radiation at doses of 0.5–1.5 Gy can lead to accelerated recovery and progress in clinical status, encephalopathy, and radiographic consolidation without any detectable acute toxicity. Although data collected so far show that LDRT could be introduced as a treatment with promising efficacy, due to limitations such as lack of randomization in most studies, we need further large-scale randomized studies, especially for elderly patients who are at greater risk of mortality due to COVID-19. However, more preclinical work and clinical trials are needed before any clear conclusion can be made.
Building Emotional Resilience: Japanese Women’s Religious and Spiritual Coping Strategies in the Time of COVID-19
Authors: Cavaliere Paola
Published: September 2021

This paper explores the moderating effect of religious and spiritual coping mechanisms on the COVID-19 pandemic-induced emotional distress among a group of Japanese women practising temple meditation and yoga. A growing body of literature identifies religion and spirituality as sources of coping mechanisms for emotional distress during the pandemic, in that they enable individuals to find ways to improve subjective well-being and quality of life. The study uses a descriptive phenomenological approach, drawing upon narratives collected between September 2020 and June 2021 from thirty-two respondents composed of a mix of religious-affiliated and self-identified non-religious women practising temple meditation and yoga. Findings indicate that more women, including religious affiliates, have favoured spiritual coping mechanisms in the forms of meditation and body–mind practices to build emotional resilience. This reflects a quest for greater subjective well-being to compensate for the increased burden of emotional care during the pandemic. Overall, while organised religions have come to appropriate more holistic forms of spirituality to respond to demands of emotional care, body–mind spiritual practices have become more appealing for younger religious and non-religious Japanese women alike, in that they downplay gender-conforming ideas of the care economy with its emphasis on dedication and dependency.
Energy Management Expert Assistant, a New Concept
Authors: Linan-Reyes Matias , Garrido-Zafra Joaquin , Gil-de-Castro Aurora , Moreno-Munoz Antonio
Published: September 2021

In recent years, interest in home energy management systems (HEMS) has grown significantly, as well as the development of Voice Assistants that substantially increase home comfort. This paper presents a novel merging of HEMS with the Assistant paradigm. The combination of both concepts has allowed the creation of a high-performance and easy-to-manage expert system (ES). It has been developed in a framework that includes, on the one hand, the efficient energy management functionality boosted with an Internet of Things (IoT) platform, where artificial intelligence (AI) and big data treatment are blended, and on the other hand, an assistant that interacts both with the user and with the HEMS itself. The creation of this ES has made it possible to optimize consumption levels, improve security, efficiency, comfort, and user experience, as well as home security (presence simulation or security against intruders), automate processes, optimize resources, and provide relevant information to the user facilitating decision making, all based on a multi-objective optimization (MOP) problem model. This paper presents both the scheme and the results obtained, the synergies generated, and the conclusions that can be drawn after 24 months of operation.
Schizophrenia Detection Using Machine Learning Approach from Social Media Content
Authors: Bae Yi Ji , Shim Midan , Lee Won Hee
Published: September 2021

Schizophrenia is a severe mental disorder that ranks among the leading causes of disability worldwide. However, many cases of schizophrenia remain untreated due to failure to diagnose, self-denial, and social stigma. With the advent of social media, individuals suffering from schizophrenia share their mental health problems and seek support and treatment options. Machine learning approaches are increasingly used for detecting schizophrenia from social media posts. This study aims to determine whether machine learning could be effectively used to detect signs of schizophrenia in social media users by analyzing their social media texts. To this end, we collected posts from the social media platform Reddit focusing on schizophrenia, along with non-mental health related posts (fitness, jokes, meditation, parenting, relationships, and teaching) for the control group. We extracted linguistic features and content topics from the posts. Using supervised machine learning, we classified posts belonging to schizophrenia and interpreted important features to identify linguistic markers of schizophrenia. We applied unsupervised clustering to the features to uncover a coherent semantic representation of words in schizophrenia. We identified significant differences in linguistic features and topics including increased use of third person plural pronouns and negative emotion words and symptom-related topics. We distinguished schizophrenic from control posts with an accuracy of 96%. Finally, we found that coherent semantic groups of words were the key to detecting schizophrenia. Our findings suggest that machine learning approaches could help us understand the linguistic characteristics of schizophrenia and identify schizophrenia or otherwise at-risk individuals using social media texts.
Proof-of-Concept of a Sensor-Based Evaluation Method for Better Sensitivity of Upper-Extremity Motor Function Assessment
Authors: Lee Seung-Hee , Hwang Ye-Ji , Lee Hwang-Jae , Kim Yun-Hee , Ogrinc Matjaž , Burdet Etienne , Kim Jong-Hyun
Published: September 2021

In rehabilitation, the Fugl–Meyer assessment (FMA) is a typical clinical instrument to assess upper-extremity motor function of stroke patients, but it cannot measure fine changes of motor function (both in recovery and deterioration) due to its limited sensitivity. This paper introduces a sensor-based automated FMA system that addresses this limitation with a continuous rating algorithm. The system consists of a depth sensor (Kinect V2) and an algorithm to rate the continuous FM scale based on fuzzy inference. Using a binary logic based classification method developed from a linguistic scoring guideline of FMA, we designed fuzzy input/output variables, fuzzy rules, membership functions, and a defuzzification method for several representative FMA tests. A pilot trial with nine stroke patients was performed to test the feasibility of the proposed approach. The continuous FM scale from the proposed algorithm exhibited a high correlation with the clinician rated scores and the results showed the possibility of more sensitive upper-extremity motor function assessment.
Security and Privacy for Mobile IoT Applications Using Blockchain
Authors: Carvalho Kevin , Granjal Jorge
Published: September 2021

Internet of Things (IoT) applications are becoming more integrated into our society and daily lives, although many of them can expose the user to threats against their privacy. Therefore, we find that it is crucial to address the privacy requirements of most of such applications and develop solutions that implement, as far as possible, privacy by design in order to mitigate relevant threats. While in the literature we may find innovative proposals to enhance the privacy of IoT applications, many of those only focus on the edge layer. On the other hand, privacy by design approaches are required throughout the whole system (e.g., at the cloud layer), in order to guarantee robust solutions to privacy in IoT. With this in mind, we propose an architecture that leverages the properties of blockchain, integrated with other technologies, to address security and privacy in the context of IoT applications. The main focus of our proposal is to enhance the privacy of the users and their data, using the anonymisation properties of blockchain to implement user-controlled privacy. We consider an IoT application with mobility for smart vehicles as our usage case, which allows us to implement and experimentally evaluate the proposed architecture and mechanisms as a proof of concept. In this application, data related to the user’s identity and location needs to be shared with security and privacy. Our proposal was implemented and experimentally validated in light of fundamental privacy and security requirements, as well as its performance. We found it to be a viable approach to security and privacy in IoT environments.
Combining Integrated Informative System and Historical Digital Twin for Maintenance and Preservation of Artistic Assets
Authors: Marra Adriana , Gerbino Salvatore , Greco Alessandro , Fabbrocino Giovanni
Published: September 2021

The protection of artistic and cultural heritage is a major challenge due to its peculiarities and its exposure to significant natural hazards. Several methodologies exist to assess the condition of artistic heritage and to protect it from exceptional actions. Moreover, novel digital technologies offer many solutions able to deliver a digital replica of artifacts of interest, so that a reduction in the uncertainties in the analysis models can be achieved. A rational approach to the preservation and protection of artistic heritage is based on traditional approaches supported and integrated by novel technologies, so that qualitative and quantitative indicators of the current condition of artistic heritage can be defined and validated in an interdisciplinary framework. The present paper reports the results of an approach to the maintenance and preservation of art objects housed in a museum complex based on a comprehensive digital path towards a Historical Digital Twin (HDT). A workflow aimed at estimating the stress regime and the dynamic properties of two sculptures, based on the detailed three-dimensional model resulting from a laser scanner survey, is illustrated and discussed. The results highlight the great advantages resulting from the integration of traditional and novel procedures in the field of conservation of artistic assets.
Selective Extraction of Platinum(IV) from the Simulated Secondary Resources Using Simple Secondary Amide and Urea Extractants
Authors: Ueda Yuki , Morisada Shintaro , Kawakita Hidetaka , Ohto Keisuke
Published: September 2021

The recycling of rare metals such as platinum (Pt) from secondary resources, such as waste electronic and electrical equipment and automotive catalysts, is an urgent global issue. In this study, simple secondary amides and urea, N-(2-ethylhexyl)acetamide, N-(2-ethylhexyl)octanamide, and 1-butyl-3-(2-ethylhexyl)urea, which selectively extract Pt(IV) from a simulated effluent containing numerous metal ions, such as in an actual hydrometallurgical process, were synthesized and achieved efficient Pt(IV) stripping using only water. Comparison of Pt(IV) extraction behavior with a tertiary amide without N–H moieties suggests that the secondary amides and urea extractants effectively use hydrogen bonding to the hexachloroplatinate anion by N–H moieties. Examining the conditions for the third phase formation revealed that the secondary amide extractant with the longest alkyl chain can be used in the extraction process for a long time without forming any third phase, despite its lower Pt(IV) extraction capacity. The practical trial with simple compounds developed in this study should contribute to the development of Pt separation and purification processes.
Determination of Tedizolid in Bacterial Growth Medium Mueller-Hinton Broth by High-Performance Liquid Chromatography and Its Application to an In Vitro Study in the Hollow-Fiber Infection Model
Authors: Iqbal Khalid , Milioudi Aliki , Martínez Elena Haro , Wicha Sebastian Georg
Published: September 2021

Pharmacokinetic/pharmacodynamic (PKPD) studies of anti-infectives are frequently performed in in vitro infection models where accurate quantification of antibiotic concentrations in bacterial growth media is crucial to establish PK/PD relationships. Here, a sensitive and rapid high-performance liquid chromatography (HPLC) method was developed to quantify tedizolid (TDZ) in the bacterial growth medium Mueller-Hinton broth (MHB). Matrix components were separated by direct protein precipitation with methanol (1:1). The chromatographic separation was carried out in a Dionex Ultimate 3000 HPLC system using an Accucore® C-18 RPMS HPLC column (2.6 µm, 100 × 2.1 mm) using isocratic elution with 25% acetonitrile and 75% of 0.1% formic acid. The lower limit of quantification was 0.03 mg/L when measured at 300 nm. Following relevant European Medicine Agency guidelines, the method was successfully validated for linearity, selectivity, recovery, inter- and intra-day precision, and accuracy and stability. When applied to in vitro PKPD studies, the method successfully quantified a range of TDZ concentration (Cmin, 0.09-Cmax, 0.65 mg/L) in MHB. The analyzed concentrations were in line with the planned PK profiles. The application of the developed method to quantify TDZ in MHB in in vitro PKPD studies is warranted.
A Systematic Review of Barriers to Accessing Undergraduate Research for STEM Students: Problematizing Under-Researched Factors for Students of Color
Authors: Pierszalowski Sophie , Bouwma-Gearhart Jana , Marlow Lindsay
Published: September 2021

While the benefits of undergraduate research experiences for students from underrepresented racial/ethnic groups have been well explored, more research is needed to better understand how students of color access these experiences. We summarize a non-structured review of literature that highlights barriers to success that students of color face in relation to STEM programming at the postsecondary level. Building from this, we report on a structured review of barriers to accessing undergraduate research. We discuss implications of the relative lack of research on access to undergraduate research for students of color at postsecondary institutions. We consider how barriers for the success and persistence of students of color in postsecondary STEM, overall, may manifest as barriers to accessing the undergraduate research experiences argued to help reduce these barriers. With the hope of guiding future relevant action, we put forth recommendations for researchers and practitioners.