bluuebunny/crossref_metadata_2025 huggingface dataset:
Environmental Analysis of Sustainable Production Practices Applied to Cyclamen and Zonal Geranium
Authors: Bonaguro Jaco , Coletto Lucia , Sambo Paolo , Nicoletto Carlo , Zanin Giampaolo
Published: January 2021

Italian floriculture is facing structural changes. Possible options to maintain competitiveness of the involved companies include promotion of added values, from local production to environmental sustainability. To quantify value and benefits of cleaner production processes and choices, a holistic view is necessary and could be provided by life cycle assessment (LCA) methodology. Previous studies on ornamental products generally focused on data from one company or a small sample. The aim of this study was a gate-to-gate life cycle assessment of two ornamental species, cyclamen (Cyclamen persicum Mill.) and zonal geranium (Pelargonium × hortorum Bailey), using data from a sample of 20 companies belonging to a floriculture district in the Treviso, Veneto region. We also assessed the potential benefits of the environmental impact of alternative management choices regarding plant protection and reuse of composted waste biomass. Life cycle impact assessment showed higher impact scores for the zonal geranium, mainly as a consequence of greenhouse heating with fossil fuels. This factor, along with higher uniformity of production practices and technological levels of equipment, translated to a lower variability in comparison with cyclamen production, which showed a wider results range, in particular for eutrophication, acidification and human toxicity potential. The application of integrated pest management with cyclamen had significant benefits by reducing acidification and human toxicity, while reducing use of mineral nutrients through amending growing media with compost resulted in a reduction in eutrophication potential. Similar achievable benefits for zonal geranium were not observed because of the dominant contribution of energy inputs.
A Spatially Distributed, Physically-Based Modeling Approach for Estimating Agricultural Nitrate Leaching to Groundwater
Authors: De Filippis Giovanna , Ercoli Laura , Rossetto Rudy
Published: January 2021

Nitrogen-nitrate, while being fundamental for crop production, is of particular concern in the agricultural sector, as it can easily leach to the water table, worsening groundwater quality. Numerical models and Geographic Information System may support the estimation of nitrate leaching rates in space and time, to support sustainable agricultural management practices. In this paper, we present a module for the simulation of the processes involved in the nitrogen cycle in the unsaturated zone, including nitrate leaching. This module was developed taking steps from the ANIMO and EPIC model frameworks and coupled to the hydrological models integrated within the FREEWAT platform. As such, the nitrogen cycle module was then included in the FREEWAT platform. The developed module and the coupling approach were tested using a simple synthetic application, where we simulated nitrate leaching through the unsaturated zone for a sunflower crop irrigated district during a dry year. The results of the simulation allow the estimation of daily nitrate concentration values at the water table. These spatially distributed values may then be further used as input concentration in models for simulating solute transport in aquifers.
Reducing Spread of Infections with a Photocatalytic Reactor—Potential Applications in Control of Hospital Staphylococcus aureus and Clostridioides difficile Infections and Inactivation of RNA Viruses
Authors: Gharaibeh Abeer , Smith Richard H. , Conway Michael J.
Published: January 2021

Contaminated surfaces and indoor environments are important sources of infectious spread within hospital and non-hospital facilities. Bacterial infections such as infections with Clostridioides (formerly Clostridium) difficile (C. difficile) and Staphylococcus aureus (S. aureus) and its antibiotic resistant strains continue to pose a significant risk to healthcare workers and patients. Additionally, the recent emergence of the coronavirus disease 2019 (COVID-19) pandemic, which is caused by the novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlights the need for safe and effective methods to decontaminate surfaces to control infection spread in hospitals and the community. To address these critical needs, we tested a photocatalytic reactor decontamination method to disinfect contaminated surfaces in a hospital and a laboratory setting. By placing the reactor in a test hospital room, growth of S. aureus and C. difficile were significantly reduced compared with a control room. Additionally, using a model enveloped positive-sense single-stranded RNA virus, dengue virus type 2 (DENV2), we showed that the use of the photocatalytic reactor reduces viral infectivity. Collectively, the results demonstrate the potential utility of photocatalytic reactors in reducing the spread of highly contagious bacterial and viral infections through contaminated surfaces and environments.
Attitudes towards Violence in Adolescents and Youth Intimate Partner Relationships: Validation of the Spanish Version of the EAV
Authors: Ortuño-Sierra Javier , García Andrea Gutiérrez , Chocarro de Luis Edurne , Pérez-Sáenz Julia , Aritio-Solana Rebeca
Published: January 2021

The main purpose of the present study was to analyze the psychometric properties of the Attitudes Scale Towards Violence (Escala de Actitudes hacia la Violencia, EAV) in adolescents. The EAV is a questionnaire devoted to assess attitudes towards violence. Additionally, the relationship between EAV and violence manifestations and depressive symptoms was analyzed. The final sample comprised a total of 1248 students in a cross-sectional survey. The EAV, the Modified Conflict Tactics Scale (M-CTS), and the Reynolds Adolescent Depression Scale (RADS) were used. The analysis of the internal structure of the EAV yielded a two-factor structure as the most adequate. The EAV scores showed measurement invariance across gender and age. The McDonald’s Omega was 0.862 and 0.872 for the two hypothesized factors. Furthermore, self-reported attitudes towards violence were associated with violence manifestations both as a victim and as a perpetrator and depressive symptoms. These results support that the EAV is a brief and easy tool to assess self-reported violence attitudes in intimate partner relationships in adolescents from the general population. The assessment of these attitudes, and its associations with violence and depressive manifestations, may help us to enhance the possibility of an early identification of adolescents potentially at risk for suffering violence as a victim or as a perpetrator.
Association of P10L Polymorphism in Melanopsin Gene with Chronic Insomnia in Mexicans
Authors: Gutiérrez-Amavizca Bianca Ethel , Prado Montes de Oca Ernesto , Gutiérrez-Amavizca Jaime Paul , Castro Oscar David , Ruíz-Marquez Cesar Heriberto , Perez Conde-Andreu Kricel , Pérez Calderón Ricardo , Aguirre Ramírez Marisela , Pérez-León Jorge Alberto
Published: January 2021

The aim of this pilot study was to determine the association of the P10L (rs2675703) polymorphism of the OPN4 gene with chronic insomnia in uncertain etiology in a Mexican population. A case control study was performed including 98 healthy subjects and 29 individuals with chronic insomnia not related to mental disorders, medical condition, medication or substance abuse. Samples were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genetic analyses showed that the T allele of P10L increased risk to chronic insomnia in a dominant model (p = 1 ×10−4; odds ratio (OR) = 9.37, CI = 8.18–335.66, Kelsey statistical power (KSP) = 99.9%), and in a recessive model (p = 7.5 × 10−5, OR = 9.37, KSP = 99.3%, CI = 2.7–34.29). In the insomnia group, we did not find a correlation between genotypes and chronotype (p = 0.219 Fisher’s exact test), severity of chronic insomnia using ISI score (p = 0.082 Fisher’s exact test) and ESS score (p ˃ 0.999 Fisher’s exact test). However, evening chronotype was correlated to daytime sleepiness severity, individuals with an eveningness chronotype had more severe drowsiness according to their insomnia severity index (ISI) score (p = 0.021 Fisher’s exact test) and Epworth sleepiness scale (ESS) score (p = 0.015 Fisher’s exact test) than the morningness and intermediate chronotype. We demonstrated that the T allele of the P10L polymorphism in the OPN4 gene is associated with chronic insomnia in Mexicans. We suggest the need to conduct larger studies in different ethnic populations to test the probable association and function of P10L and other SNPs in the OPN4 gene and in the onset of chronic insomnia.
Sex-Specific Alterations in Cardiac DNA Methylation in Adult Mice by Perinatal Lead Exposure
Authors: Svoboda Laurie K. , Wang Kai , Jones Tamara R. , Colacino Justin A. , Sartor Maureen A. , Dolinoy Dana C.
Published: January 2021

Environmental factors play an important role in the etiology of cardiovascular diseases. Cardiovascular diseases exhibit marked sexual dimorphism; however, the sex-specific effects of environmental exposures on cardiac health are incompletely understood. Perinatal and adult exposures to the metal lead (Pb) are linked to several adverse cardiovascular outcomes, but the sex-specific effects of this toxicant on the heart have received little attention. Perinatal environmental exposures can lead to disease through disruption of the normal epigenetic programming that occurs during early development. Using a mouse model of human-relevant perinatal environmental exposure, we investigated the effects of exposure to Pb during gestation and lactation on DNA methylation in the hearts of adult offspring mice (n = 6 per sex). Two weeks prior to mating, dams were assigned to control or Pb acetate (32 ppm) water, and exposure continued until offspring were weaned at three weeks of age. Enhanced reduced-representation bisulfite sequencing was used to measure DNA methylation in the hearts of offspring at five months of age. Although Pb exposure stopped at three weeks of age, we discovered hundreds of differentially methylated cytosines (DMCs) and regions (DMRs) in males and females at five months of age. DMCs/DMRs and their associated genes were sex-specific, with a small, but statistically significant subset overlapping between sexes. Pathway analysis revealed altered methylation of genes important for cardiac and other tissue development in males, and histone demethylation in females. Together, these data demonstrate that perinatal exposure to Pb induces sex-specific changes in cardiac DNA methylation that are present long after cessation of exposure, and highlight the importance of considering sex in environmental epigenetics and mechanistic toxicology studies.
Psychological Impact on the Nursing Professionals of the Rioja Health Service (Spain) Due to the SARS-CoV-2 Virus
Authors: Del Pozo-Herce Pablo , Garrido-García Rebeca , Santolalla-Arnedo Iván , Gea-Caballero Vicente , García-Molina Pablo , Ruiz de Viñaspre-Hernández Regina , Rodríguez-Velasco Francisco José , Juárez-Vela Raúl
Published: January 2021

Background: The COVID-19 pandemic is a public health emergency that has affected health professionals around the world, causing physical and mental exhaustion with a greater probability of developing mental disorders in professionals who provide healthcare. Objective: The objective of this study was to know the psychological impact of the SARS-CoV-2 virus on the nursing professionals working for the Rioja Health Service. Methods: We conducted an observational and descriptive cross-sectional study. The nursing staff at the Rioja Health Service were invited to respond to a self-administered questionnaire between June and August 2020. Results: A total of 605 health professionals participated in the questionnaire; 91.9% were women, 63.14% were registered nurses, and 36.28% were auxiliary nurses. Risk factors for mental health professionals were identified in more than 90% of nurses (p = 0.009), affecting their psychological state with feelings of exhaustion, emotional overload (p = 0.002), and less use of coping strategies among women. Younger professionals with less experience had higher levels of stress compared to those with more than five years of experience, who showed a progressive reduction in the impact of stressors (p < 0.001). Professionals with dependent family members presented higher levels of emotional overload and coping problems (p = 0.009). Conclusion: The COVID-19 pandemic has had a significant psychological impact on health professionals in terms of stress, emotional well-being, and the use of coping strategies. Female health professionals with dependents, a temporary contract, and less work experience have been more psychologically affected.
Gambling Self-Control Strategies: A Qualitative Analysis
Authors: Flores-Pajot Marie-Claire , Atif Sara , Dufour Magali , Brunelle Natacha , Currie Shawn R. , Hodgins David C. , Nadeau Louise , Young Matthew M.
Published: January 2021

There is limited research exploring the perceptions of people who gamble on the self-control strategies used to limit their gambling. This qualitative study examines self-control strategies used to limit money spent gambling, frequency of gambling, and time spent gambling. A total of 56 people who gamble (27 males and 29 females) participated in nine focus groups and five individual interviews in Montreal, Calgary, and Toronto (Canada). Self-control strategies used to limit their gambling expenditure were more common than frequency or time limiting strategies. Strategies to limit expenditure included: restricting access to money; keeping track of money allocated to gambling activities; and avoiding certain types of gambling activities. Various contextual factors were identified to influence those strategies, including social influences; winning or losing; using substances. Findings from this study emphasize the importance of communicating clear gambling limits to people who gamble, as well as the value of developing individual self-control strategies to limit frequency, time and money spent gambling.
Early Life Exposure to Aflatoxin B1 in Rats: Alterations in Lipids, Hormones, and DNA Methylation among the Offspring
Authors: Rotimi Oluwakemi A. , Onuzulu Chinonye D. , Dewald Alisa L. , Ehlinger Jessa , Adelani Isaacson B. , Olasehinde Olutola E. , Rotimi Solomon O. , Goodrich Jaclyn M.
Published: January 2021

Aflatoxins are toxic compounds produced by molds of the Aspergillus species that contaminate food primarily in tropical countries. The most toxic aflatoxin, aflatoxin B1 (AFB1), is a major cause of hepatocellular carcinoma (HCC) in these countries. In sub-Saharan Africa, aflatoxin contamination is common, and perinatal AFB1 exposure has been linked to the early onset of HCC. Epigenetic programming, including changes to DNA methylation, is one mechanism by which early life exposures can lead to adult disease. This study aims to elucidate whether perinatal AFB1 exposure alters markers of offspring health including weight, lipid, and hormone profiles as well as epigenetic regulation that may later influence cancer risk. Pregnant rats were exposed to two doses of AFB1 (low 0.5 and high 5 mg/kg) before conception, throughout pregnancy, and while weaning and compared to an unexposed group. Offspring from each group were followed to 3 weeks or 3 months of age, and their blood and liver samples were collected. Body weights and lipids were assessed at 3 weeks and 3 months while reproductive, gonadotropic, and thyroid hormones were assessed at 3 months. Prenatal AFB1 (high dose) exposure resulted in significant 16.3%, 31.6%, and 7.5% decreases in weight of the offspring at birth, 3 weeks, and 3 months, respectively. Both doses of exposure altered lipid and hormone profiles. Pyrosequencing was used to quantify percent DNA methylation at tumor suppressor gene Tp53 and growth-regulator H19 in DNA from liver and blood. Results were compared between the control and AFB1 exposure groups in 3-week liver samples and 3-week and 3-month blood samples. Relative to controls, Tp53 DNA methylation in both low- and high-dose exposed rats was significantly decreased in liver samples and increased in the blood (p < 0.05 in linear mixed models). H19 methylation was higher in the liver from low- and high-exposed rats and decreased in 3-month blood samples from the high exposure group (p < 0.05). Further research is warranted to determine whether such hormone, lipid, and epigenetic alterations from AFB1 exposure early in life play a role in the development of early-onset HCC.
The Role of Perfectionistic Self-Presentation in Pediatric Pain
Authors: Sánchez-Rodríguez Elisabet , Ferreira-Valente Alexandra , Pathak Anupa , Solé Ester , Sharma Saurab , Jensen Mark P. , Miró Jordi
Published: January 2021

This study sought to better understand the associations between perfectionistic self-presentation and measures of pain intensity, pain catastrophizing, pain interference, and fatigue in children and adolescents with pain. In the study, 218 adolescents responded to measures of perfectionistic self-presentation (i.e., perfectionistic self-promotion, nondisplay of imperfection and nondisclosure of imperfection), pain intensity, pain catastrophizing, pain interference, and fatigue. Four hierarchical regression analyses and three mediation analyses were conducted. Our results showed that perfectionistic self-promotion was significantly and independently associated with pain intensity and that nondisplay of imperfection was significantly and independently associated with pain catastrophizing, pain interference, and fatigue. Nondisclosure of imperfection was not significantly associated with any criterion variable. Pain catastrophizing mediated the association between both perfectionistic self-presentation and nondisplay imperfection and pain interference but not between nondisclosure of imperfection and pain interference. The findings provide new information about the role of perfectionistic self-presentation in children and adolescents’ experience of pain. These findings, if replicated, support perfectionism as a potential target of pain treatment in young people.
Selective Sulfur Dioxide Absorption from Simulated Flue Gas Using Various Aqueous Alkali Solutions in a Polypropylene Hollow Fiber Membrane Contactor: Removal Efficiency and Use of Sulfur Dioxide
Authors: Park Hyun Sic , Kang Dongwoan , Kang Jo Hong , Kim Kwanghwi , Kim Jaehyuk , Song Hojun
Published: January 2021

Hollow fiber membrane contactors (HFMCs) provide a large specific surface area. Thus, their significantly reduced volume provides an advantage compared to the conventional gas–liquid contactor. In this study, the selective removal efficiency of flue gas, in which sulfur oxide (SO2) and carbon dioxide (CO2) coexist, was measured using a polypropylene (PP) HFMC with such advantages. To increase the selective removal efficiency of SO2, experiments were conducted using various alkaline absorbents. As a result, with 0.05 M ammonia solution, the removal efficiency of 95% or more was exhibited with continuous operation for 100 h or more. We confirmed that the absorbent saturated by the once-through mode was aqueous ammonium sulfate ((NH4)2SO4) solution and could be used as a fertilizer without additional processing.
Lighting in the Home and Health: A Systematic Review
Authors: Osibona Oluwapelumi , Solomon Bethlehem D. , Fecht Daniela
Published: January 2021

Poor housing is an important determinant of poor health. One key aspect of housing quality is lighting. Light is important for visual performance and safety, and also plays a vital role in regulating human physiological functions. This review aims to synthesise existing evidence on the relationship between lighting in the home and health and recommends areas for future research. Three databases were searched for relevant literature using pre-defined inclusion criteria. Study quality was assessed using the Newcastle Ottawa Scale. Extracted data were qualitatively synthesised according to type of lighting (natural light, artificial light and light at night) and stratified by broad health domains (physical, mental and sleep health). Of the 4043 records retrieved, 28 studies met the inclusion criteria. There was considerable heterogeneity in light exposure metrics used and specific health outcome assessed by the studies. Lighting in the home can negatively affect health but the current evidence base is limited to a small number of studies in different domains of light and health. Further research surrounding specific health outcomes is required to better inform housing quality assessments and lighting practises in the home.
Validation of a Self-Perceived Adaptive Behaviors Scale in Older Chilean Women and Percentiles for Evaluation
Authors: Gomez-Campos Rossana , Vidal-Espinoza Ruben , Castelli Correia de Campos Luis Felipe , Lee-Andruske Cynthia , Sulla-Torres Jose , Cornejo-Valderrama Carolina , Lepe-Martinez Nancy , Lagos-Luciano Juan , Monne de la Peña Rodrigo , Urra-Albornoz Camilo , Pezoa-Fuentes Paz , Cossio-Bolaños Marco
Published: January 2021

Healthy ageing means optimizing opportunities that allow older individuals to participate actively in society without discrimination. Learning adaptive behaviors (AB) may be extremely important for individuals for all stages of life. The goal of this study was: (a) to create a scale for self-perceived adaptive behavior, and (b) propose percentiles for evaluating AB in older adult women. A self-perception scale was created to measure adaptive behavior. Anthropometric and physical fitness variables for 192 older Chilean women (ages 60 to 88) were collected and evaluated. Content validity reflected agreement from 0.75 to 1.0. Construct validity carried out with exploratory factor analysis (EFA) resulted in 11 dimensions with 62 items in groups. Saturation oscillated between 0.62 and 0.85 with the explanation of variation as 46.27%. Cronbach’s Alpha was r = 0.83. The results indicated that the scale developed was valid and reliable for the Chilean women studied. This scale may be used to measure self-perception of AB patterns in older women. Furthermore, the percentiles allow for classification of the AB by age and anthropometric indices.
Ecological Grief as a Response to Environmental Change: A Mental Health Risk or Functional Response?
Authors: Comtesse Hannah , Ertl Verena , Hengst Sophie M. C. , Rosner Rita , Smid Geert E.
Published: January 2021

The perception of the impact of climate change on the environment is becoming a lived experience for more and more people. Several new terms for climate change-induced distress have been introduced to describe the long-term emotional consequences of anticipated or actual environmental changes, with ecological grief as a prime example. The mourning of the loss of ecosystems, landscapes, species and ways of life is likely to become a more frequent experience around the world. However, there is a lack of conceptual clarity and systematic research efforts with regard to such ecological grief. This perspective article introduces the concept of ecological grief and contextualizes it within the field of bereavement. We provide a case description of a mountaineer in Central Europe dealing with ecological grief. We introduce ways by which ecological grief may pose a mental health risk and/or motivate environmental behavior and delineate aspects by which it can be differentiated from related concepts of solastalgia and eco-anxiety. In conclusion, we offer a systematic agenda for future research that is embedded in the context of disaster mental health and bereavement research.
The Moderating Role of Parental Sleep Knowledge on Children with Developmental Disabilities and Their Parents’ Sleep
Authors: Halstead Elizabeth J. , Jones Alexandra , Esposito Gianluca , Dimitriou Dagmara
Published: January 2021

Background: Children with intellectual and developmental difficulties often experience sleep problems, which in turn may impact parental sleep patterns. This study explored the role of parental sleep knowledge as a moderator on the relationship between child sleep and parental sleep impairment. Methods: 582 parents or caregivers (92.6% mothers) of children with different developmental disabilities (Age M = 9.34, 29.5% females) such as Down’s syndrome, participated in an online survey. Multiple regression analysis was conducted. Results: Parental sleep knowledge of child sleep was a moderating variable in the relationship between child sleep nocturnal duration and parental sleep impairment. Although overall, sleep knowledge was high in this sample, two specific knowledge gaps were identified namely child sleep duration requirements, and the recognition of signs of a well-rested child. Conclusion: This study has provided evidence that increased parental sleep knowledge can positively impact both child and parental sleep outcomes.
Role for Physical Fitness in the Association between Age and Cognitive Function in Older Adults: A Mediation Analysis of the SABE Colombia Study
Authors: Pérez-Sousa Miguel Ángel , del Pozo-Cruz Jesús , Olivares Pedro R. , Cano-Gutiérrez Carlos A. , Izquierdo Mikel , Ramírez-Vélez Robinson
Published: January 2021

Objectives. We investigated the association between physical fitness and cognitive status. Further, we examined whether physical fitness mediates the association between cognitive functioning and aging. Design. Cross-sectional study. Setting. Urban and rural Colombian older adults. Methods. 4416 participants from the SABE study were included in the current analysis. Physical fitness was assessed with the handgrip test and the usual gait speed test. Cognitive status was evaluated through the Folstein Mini-Mental State Examination. A parallel mediation path was used to test the possible mediator role of physical fitness between aging and cognitive functioning. Results. Older adults with lower handgrip strength (HGS) were more likely to have mild-cognitive status than older adults with healthy HGS (OR = 1.53, 95% CI = 1.15; 2.02). In addition, older adults with a slower gait speed were more likely to have mild cognitive impairment (OR = 2.05, 95% CI = 1.54; 2.78). Age had an inverse relationship with cognitive function (β = −0.110, 95% CI = −0.130; −0.100) and it was also inversely associated with HGS (β = −0.003, 95% CI = −0.005; −0.002) and gait speed (β = −0.010, 95% CI = −0.011; −0.009). The indirect effects, which indicate that the effect of age on cognitive function is transmitted through mediators, showed that both gait speed (β = −0.028, 95% CI = −0.036; −0.020) and HGS (β = −0.014, 95% CI = −0.024; −0.005) were independent mediators of the detrimental effect of aging on cognitive function. Conclusions. Physical fitness mediates the effects of aging on cognitive functioning. Our findings suggest that physical activity can be a key factor to prevent cognitive deterioration during aging process.
Understanding the Gap between Environmental Intention and Pro-Environmental Behavior towards the Waste Sorting and Management Policy of China
Authors: Wang Huilin , Mangmeechai Aweewan
Published: January 2021

Environmental pollution and ecological damage caused by human activities have attracted widespread attention in recent years, and while citizens’ environmental awareness and intentions have increased, their actions may not necessarily change accordingly. This study aims to understand the intention–behavior gap, based on the theory of planned behavior (TPB), by exploring the relationship between intention and pro-environmental behavior on the new waste sorting policy in China. The structural model of extended TPB was tested using sample data from 3113 residents of Changsha, each of whom was asked to complete a two-stage survey. Results demonstrated that perceived policy effectiveness and actual behavioral control positively affect behavioral intention, implementation intention, and pro-environmental behavior. Among them, the actual behavioral control of residents was found to be the most influential factor on behavioral intention and implementation intention, followed by residents’ perceived policy effectiveness. Moreover, behavioral intention and implementation intention mediate the relationship between antecedents and pro-environmental behavior. These findings imply that people with high-level perceptions of policy effectiveness, strong control over actual behavior, strong behavioral intentions, and strong implementation intentions are more likely to engage in pro-environmental behavior. The findings suggest that factors such as perceived policy effectiveness and actual behavioral control should be considered when implementing new policies and campaigns for waste sorting and management.
JAZF1, A Novel p400/TIP60/NuA4 Complex Member, Regulates H2A.Z Acetylation at Regulatory Regions
Authors: Procida Tara , Friedrich Tobias , Jack Antonia P. M. , Peritore Martina , Bönisch Clemens , Eberl H. Christian , Daus Nadine , Kletenkov Konstantin , Nist Andrea , Stiewe Thorsten , Borggrefe Tilman , Mann Matthias , Bartkuhn Marek , Hake Sandra B.
Published: January 2021

Histone variants differ in amino acid sequence, expression timing and genomic localization sites from canonical histones and convey unique functions to eukaryotic cells. Their tightly controlled spatial and temporal deposition into specific chromatin regions is accomplished by dedicated chaperone and/or remodeling complexes. While quantitatively identifying the chaperone complexes of many human H2A variants by using mass spectrometry, we also found additional members of the known H2A.Z chaperone complexes p400/TIP60/NuA4 and SRCAP. We discovered JAZF1, a nuclear/nucleolar protein, as a member of a p400 sub-complex containing MBTD1 but excluding ANP32E. Depletion of JAZF1 results in transcriptome changes that affect, among other pathways, ribosome biogenesis. To identify the underlying molecular mechanism contributing to JAZF1’s function in gene regulation, we performed genome-wide ChIP-seq analyses. Interestingly, depletion of JAZF1 leads to reduced H2A.Z acetylation levels at > 1000 regulatory sites without affecting H2A.Z nucleosome positioning. Since JAZF1 associates with the histone acetyltransferase TIP60, whose depletion causes a correlated H2A.Z deacetylation of several JAZF1-targeted enhancer regions, we speculate that JAZF1 acts as chromatin modulator by recruiting TIP60’s enzymatic activity. Altogether, this study uncovers JAZF1 as a member of a TIP60-containing p400 chaperone complex orchestrating H2A.Z acetylation at regulatory regions controlling the expression of genes, many of which are involved in ribosome biogenesis.
Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects
Authors: Salava Hymavathi , Thula Sravankumar , Mohan Vijee , Kumar Rahul , Maghuly Fatemeh
Published: January 2021

Plants regularly face the changing climatic conditions that cause biotic and abiotic stress responses. The abiotic stresses are the primary constraints affecting crop yield and nutritional quality in many crop plants. The advances in genome sequencing and high-throughput approaches have enabled the researchers to use genome editing tools for the functional characterization of many genes useful for crop improvement. The present review focuses on the genome editing tools for improving many traits such as disease resistance, abiotic stress tolerance, yield, quality, and nutritional aspects of tomato. Many candidate genes conferring tolerance to abiotic stresses such as heat, cold, drought, and salinity stress have been successfully manipulated by gene modification and editing techniques such as RNA interference, insertional mutagenesis, and clustered regularly interspaced short palindromic repeat (CRISPR/Cas9). In this regard, the genome editing tools such as CRISPR/Cas9, which is a fast and efficient technology that can be exploited to explore the genetic resources for the improvement of tomato and other crop plants in terms of stress tolerance and nutritional quality. The review presents examples of gene editing responsible for conferring both biotic and abiotic stresses in tomato simultaneously. The literature on using this powerful technology to improve fruit quality, yield, and nutritional aspects in tomato is highlighted. Finally, the prospects and challenges of genome editing, public and political acceptance in tomato are discussed.
Neurofibromatosis Type 2 (NF2) and the Implications for Vestibular Schwannoma and Meningioma Pathogenesis
Authors: Bachir Suha , Shah Sanjit , Shapiro Scott , Koehler Abigail , Mahammedi Abdelkader , Samy Ravi N. , Zuccarello Mario , Schorry Elizabeth , Sengupta Soma
Published: January 2021

Patients diagnosed with neurofibromatosis type 2 (NF2) are extremely likely to develop meningiomas, in addition to vestibular schwannomas. Meningiomas are a common primary brain tumor; many NF2 patients suffer from multiple meningiomas. In NF2, patients have mutations in the NF2 gene, specifically with loss of function in a tumor-suppressor protein that has a number of synonymous names, including: Merlin, Neurofibromin 2, and schwannomin. Merlin is a 70 kDa protein that has 10 different isoforms. The Hippo Tumor Suppressor pathway is regulated upstream by Merlin. This pathway is critical in regulating cell proliferation and apoptosis, characteristics that are important for tumor progression. Mutations of the NF2 gene are strongly associated with NF2 diagnosis, leading to benign proliferative conditions such as vestibular schwannomas and meningiomas. Unfortunately, even though these tumors are benign, they are associated with significant morbidity and the potential for early mortality. In this review, we aim to encompass meningiomas and vestibular schwannomas as they pertain to NF2 by assessing molecular genetics, common tumor types, and tumor pathogenesis.
Plant Growth-Defense Trade-Offs: Molecular Processes Leading to Physiological Changes
Authors: Figueroa-Macías Juan Pablo , García Yamilet Coll , Núñez María , Díaz Katy , Olea Andres F. , Espinoza Luis
Published: January 2021

In order to survive in a hostile habitat, plants have to manage the available resources to reach a delicate balance between development and defense processes, setting up what plant scientists call a trade-off. Most of these processes are basically responses to stimuli sensed by plant cell receptors and are influenced by the environmental features, which can incredibly modify such responses and even cause changes upon both molecular and phenotypic level. Therefore, significant differences can be detected between plants of the same species living in different environments. The comprehension of plant growth-defense trade-offs from the molecular basis to the phenotypic expression is one of the fundamentals for developing sustainable agriculture, so with this review we intend to contribute to the increasing of knowledge on this topic, which have a great importance for future development of agricultural crop production.
Some Molecular and Cellular Stress Mechanisms Associated with Neurodegenerative Diseases and Atherosclerosis
Authors: Sazonova Margarita A. , Sinyov Vasily V. , Ryzhkova Anastasia I. , Sazonova Marina D. , Kirichenko Tatiana V. , Khotina Victoria A. , Khasanova Zukhra B. , Doroschuk Natalya A. , Karagodin Vasily P. , Orekhov Alexander N. , Sobenin Igor A.
Published: January 2021

Chronic stress is a combination of nonspecific adaptive reactions of the body to the influence of various adverse stress factors which disrupt its homeostasis, and it is also a corresponding state of the organism’s nervous system (or the body in general). We hypothesized that chronic stress may be one of the causes occurence of several molecular and cellular types of stress. We analyzed literary sources and considered most of these types of stress in our review article. We examined genes and mutations of nuclear and mitochondrial genomes and also molecular variants which lead to various types of stress. The end result of chronic stress can be metabolic disturbance in humans and animals, leading to accumulation of reactive oxygen species (ROS), oxidative stress, energy deficiency in cells (due to a decrease in ATP synthesis) and mitochondrial dysfunction. These changes can last for the lifetime and lead to severe pathologies, including neurodegenerative diseases and atherosclerosis. The analysis of literature allowed us to conclude that under the influence of chronic stress, metabolism in the human body can be disrupted, mutations of the mitochondrial and nuclear genome and dysfunction of cells and their compartments can occur. As a result of these processes, oxidative, genotoxic, and cellular stress can occur. Therefore, chronic stress can be one of the causes forthe occurrence and development of neurodegenerative diseases and atherosclerosis. In particular, chronic stress can play a large role in the occurrence and development of oxidative, genotoxic, and cellular types of stress.
Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.)
Authors: Mareri Lavinia , Guerriero Gea , Hausman Jean-Francois , Cai Giampiero
Published: January 2021

Sucrose synthase is a key enzyme in sucrose metabolism as it saves an important part of sucrose energy in the uridine-5′-diphosphate glucose (UDP-glucose) molecule. As such it is also involved in the synthesis of fundamental molecules such as callose and cellulose, the latter being present in all cell walls of plant cells and therefore also in the gelatinous cell walls of sclerenchyma cells such as bast fibers. Given the importance of these cells in plants of economic interest such as hemp, flax and nettle, in this work we have studied the occurrence of Sucrose synthase in nettle stems by analyzing its distribution between the cytosol, membranes and cell wall. We have therefore developed a purification protocol that can allow the analysis of various characteristics of the enzyme. In nettle, Sucrose synthase is encoded by different genes and each form of the enzyme could be subjected to different post-translational modifications. Therefore, by two-dimensional electrophoresis analysis, we have also traced the phosphorylation profile of Sucrose synthase isoforms in the various cell compartments. This information paves the way for further investigation of Sucrose synthase in plants such as nettle, which is both economically important, but also difficult to study.
Chronic Lorcaserin Treatment Reverses the Nicotine Withdrawal-Induced Disruptions to Behavior and Maturation in Developing Neurons in the Hippocampus of Rats
Authors: Zaniewska Magdalena , Nikiforuk Agnieszka , Głowacka Urszula , Brygider Sabina , Wesołowska Julita , Litwa Ewa , Maćkowiak Marzena
Published: January 2021

Preclinical data have shown that treatment with serotonin (5-HT)2C receptor agonists inhibits the behavioral effects of nicotine, including self-administration, reinstatement, and locomotor responses to nicotine. Since the data on the effects of 5-HT2C receptor agonism on nicotine withdrawal signs are limited, we aimed to investigate whether 5-HT2C receptor agonism alleviated the behavioral and neurobiochemical (hippocampal neurogenesis) consequences of nicotine withdrawal in Sprague-Dawley rats. Our data indicate that withdrawal from nicotine self-administration induced locomotor hyperactivity, lengthened immobility time (the forced swim test), induced ‘drug-seeking’ behavior and deficits in cognition-like behavior (the novel object recognition task). A two-week exposure to the 5-HT2C receptor agonist lorcaserin attenuated locomotor hyperactivity and induced recovery from depression-like behavior. Analyses of brain slices from nicotine-withdrawn animals revealed that lorcaserin treatment recovered the reduced number of doublecortin (DCX)-positive cells, but it did not affect the number of Ki-67- or 5-bromo-2’-deoxyuridine (BrdU)-positive cells or the maturation of proliferating neurons in drug-weaned rats. To summarize, we show that lorcaserin alleviated locomotor responses and depression-like state during nicotine withdrawal. We propose that the modulatory effect of lorcaserin on the ‘affective’ aspects of nicotine cessation may be linked to the positive changes caused by the compound in hippocampal neurogenesis during nicotine withdrawal.
Carboxypeptidase U (CPU, TAFIa, CPB2) in Thromboembolic Disease: What Do We Know Three Decades after Its Discovery?
Authors: Claesen Karen , Mertens Joachim C. , Leenaerts Dorien , Hendriks Dirk
Published: January 2021

Procarboxypeptidase U (proCPU, TAFI, proCPB2) is a basic carboxypeptidase zymogen that is converted by thrombin(-thrombomodulin) or plasmin into the active carboxypeptidase U (CPU, TAFIa, CPB2), a potent attenuator of fibrinolysis. As CPU forms a molecular link between coagulation and fibrinolysis, the development of CPU inhibitors as profibrinolytic agents constitutes an attractive new concept to improve endogenous fibrinolysis or to increase the efficacy of thrombolytic therapy in thromboembolic diseases. Furthermore, extensive research has been conducted on the in vivo role of CPU in (the acute phase of) thromboembolic disease, as well as on the hypothesis that high proCPU levels and the Thr/Ile325 polymorphism may cause a thrombotic predisposition. In this paper, an overview is given of the methods available for measuring proCPU, CPU, and inactivated CPU (CPUi), together with a summary of the clinical data generated so far, ranging from the current knowledge on proCPU concentrations and polymorphisms as potential thromboembolic risk factors to the positioning of different CPU forms (proCPU, CPU, and CPUi) as diagnostic markers for thromboembolic disease, and the potential benefit of pharmacological inhibition of the CPU pathway.
Aesculus hippocastanum L. as a Stabilizer in Hemp Seed Oil Nanoemulsions for Potential Biomedical and Food Applications
Authors: Jarzębski Maciej , Smułek Wojciech , Siejak Przemysław , Rezler Ryszard , Pawlicz Jarosław , Trzeciak Tomasz , Jarzębska Małgorzata , Majchrzak Oliwia , Kaczorek Ewa , Kazemian Pardis , Ponieważ-Pawlicz Marta , Fathordoobady Farahnaz
Published: January 2021

Nanoemulsion systems receive a significant amount of interest nowadays due to their promising potential in biomedicine and food technology. Using a two-step process, we produced a series of nanoemulsion systems with different concentrations of hemp seed oil (HSO) stabilized with Aesculus hippocastanum L. extract (AHE). Water and commercially-available low-concentrated hyaluronic acid (HA) were used as the liquid phase. Stability tests, including an emulsifying index (EI), and droplet size distribution tests performed by dynamic light scattering (DLS) proved the beneficial impact of AHE on the emulsion’s stability. After 7 days of storage, the EI for the water-based system was found to be around 100%, unlike the HA systems. The highest stability was achieved by an emulsion containing 5% HSO and 2 g/L AHE in water, as well as the HA solution. In order to obtain the detailed characteristics of the emulsions, UV-Vis and FTIR spectra were recorded, and the viscosity of the samples was determined. Finally, a visible microscopic analysis was used for the homogeneity evaluation of the samples, and was compared with the DLS results of the water system emulsion, which showed a desirable stability. The presented results demonstrate the possible use of oil emulsions based on a plant extract rich in saponins, such as AHE. Furthermore, it was found that the anti-inflammatory properties of AHE provide opportunities for the development of new emulsion formulations with health benefits.
Microstructured Lipid Carriers (MLC) Based on N-Acetylcysteine and Chitosan Preventing Pseudomonas aeruginosa Biofilm
Authors: Guerini Marta , Grisoli Pietro , Pane Cristina , Perugini Paola
Published: January 2021

The aim of this work was the development of microstructured lipid carriers (MLC) based on chitosan (CH) and containing N-acetylcysteine (NAC), a mucolytic and antioxidant agent, to inhibit the formation of Pseudomonas aeruginosa biofilm. MLC were prepared using the high shear homogenization technique. The MLC were characterized for morphology, particle size, Z potential, encapsulation efficiency and drug release. The antioxidant properties of NAC-loaded microstructured carriers were evaluated through an in vitro spectrophotometer assay. Finally, the activity of NAC-CH-MLC on biofilm production by Pseudomonas aeruginosa was also evaluated. Results obtained from this study highlighted that the use of chitosan into the inner aqueous phase permitted to obtain microstructured particles with a narrow size range and with good encapsulation efficiency. NAC-loaded MLC showed higher antioxidant activity than the free molecule, demonstrating how encapsulation increases the antioxidant effect of the molecule. Furthermore, the reduction of biofilm growth resulted extremely high with MLC being 64.74% ± 6.2% and 83.74% ± 9.95%, respectively, at 0.5 mg/mL and 2 mg/mL. In conclusion, this work represents a favorable technological strategy against diseases in which bacterial biofilm is relevant, such as cystic fibrosis.
Insights into the Role of Tick Salivary Protease Inhibitors during Ectoparasite–Host Crosstalk
Authors: Jmel Mohamed Amine , Aounallah Hajer , Bensaoud Chaima , Mekki Imen , Chmelař Jindřich , Faria Fernanda , M’ghirbi Youmna , Kotsyfakis Michalis
Published: January 2021

Protease inhibitors (PIs) are ubiquitous regulatory proteins present in all kingdoms. They play crucial tasks in controlling biological processes directed by proteases which, if not tightly regulated, can damage the host organism. PIs can be classified according to their targeted proteases or their mechanism of action. The functions of many PIs have now been characterized and are showing clinical relevance for the treatment of human diseases such as arthritis, hepatitis, cancer, AIDS, and cardiovascular diseases, amongst others. Other PIs have potential use in agriculture as insecticides, anti-fungal, and antibacterial agents. PIs from tick salivary glands are special due to their pharmacological properties and their high specificity, selectivity, and affinity to their target proteases at the tick–host interface. In this review, we discuss the structure and function of PIs in general and those PI superfamilies abundant in tick salivary glands to illustrate their possible practical applications. In doing so, we describe tick salivary PIs that are showing promise as drug candidates, highlighting the most promising ones tested in vivo and which are now progressing to preclinical and clinical trials.
Correction: Abughanam, G., et al. Mesenchymal Stem Cells Extract (MSCsE)-Based Therapy Alleviates Xerostomia and Keratoconjunctivitis Sicca in Sjogren’s Syndrome-Like Disease. Int. J. Mol. Sci. 2019, 20, 4750
Authors: Abughanam Ghada , Elkashty Osama A. , Liu Younan , Bakkar Mohammed O. , Tran Simon D.
Published: January 2021

The authors wish to make the following corrections to this paper [...]