bluuebunny/crossref_metadata_2025 huggingface dataset:
The Construction of Ideological and Political Education in Higher Vocational Schools Based on Smartphone Carriers
Authors: Chen Wei
Published: April 2022

In the age of technology, artificial intelligence has reached new heights. The growth of the educational system has also evolved with technological growth. In this research, people are going to study the construction of ideological and political education in higher vocational schools based on smartphone carriers. The use of smartphones is becoming more common and unavoidable due to the growth of Internet technology, cloud technology, and wireless technology. The availability of 4G and 5G technologies supports all the relevant and nonrelevant industries to utilize this growth. The field of education has also adapted to this new age of technology by implementing online classes that replace classes that were traditionally taken in school classrooms. Mobile phones serve the purpose of helping students learn via various means, such as educational apps, educational websites, and video conferencing classes, also called e-classes. Wireless networks are available almost everywhere. With the help of this wireless technology and technology like 5G, smartphone usage has increased. Using deep learning models, wireless networks, Internet technology, and smartphones, a system has been proposed that could be used to build ideological and political education in high-tech schools.
On One Generalization of the Multipoint Nonlocal Contact Problem for Elliptic Equation in Rectangular Area
Authors: Davitashvili Tinatin , Meladze Hamlet , Criado-Aldeanueva Francisco , Sanchez Jose Maria
Published: April 2022

Nonlocal contact problem for two‐dimensional linear elliptic equations is stated and investigated. The method of separation of variables is used to find the solution of a stated problem in the case of Poisson’s equation. Then, the more general problem with nonlocal multipoint contact conditions for elliptic equation with variable coefficients is considered, and the iterative method to solve the problem numerically is constructed and investigated. The uniqueness and existence of the regular solution are proved. The iterative method allows reducing the solution of a nonlocal contact problem to the solution of a sequence of classical boundary value problems.
Modeling and Analysis of Automotive Engine Crankshaft Made of Composite and Functionally Graded Materials
Authors: Asiri Saeed
Published: April 2022

With the consistent advancement and technical improvisation in the automobile industry, the researchers are working on the material optimization techniques to select and utilize the materials effective in power transmission. It is well know that power transmission systems in the mechanical industries and, namely, in automotive machinaries, require a durable material with proper properties to ensure the optimum operational conditions during power transmission. The material utilized in the driving shaft must have a proper endurance and considerable yielding limit that means they have that ability to sustain the fatigue in the power transmission elements. The method of study is that engine shafts are a major part of the automobiles and the production of these shafts is an important requirement for the resistance against bending and torsion. The torsion and bending resisting moments are important before selecting the material. As a result, selecting the optimal material and surface treatment procedure to offer the highest performance for the shaft is crucial. Wear resistance and corrosion resistance are the two most important factors to consider when selecting a material for a surface. This paper is able to show the effect of materials of the crankshaft on those two elements. The research discusses the shafts of three different types of materials: homogeneous, composite, and functionally graded materials (FGM). For the optimum performance of the vehicle engine shaft, FGM is the ideal material to consider. It has been shown that the performance of crankshaft was improved in case of FGM. Based on the results of the modal and harmonic analysis, it is concluded that FGM crankshaft would offer the best durability and show optimum performance when compared with the other two material crankshafts investigated in this study.
Hydroxyacid Oxidase 2 (HAO2) Inhibits the Tumorigenicity of Hepatocellular Carcinoma and Is Negatively Regulated by miR-615-5p
Authors: Li Yuxuan , Zhang Mingchao , Li Xiuling , Wang Yanyan , Wang Yu , Li Yuan , Zhu Huihui , Ding Hui , Li Xiaofang , Sun Suofeng
Published: April 2022

Background. Hepatocellular carcinoma (HCC) is the sixth most common kind of cancer worldwide and the third leading cause of cancer mortality. Although a few studies have shown that hydroxyacid oxidase 2 (HAO2) may prevent HCC development, the molecular mechanism is unclear. Methods. We examined the levels of HAO2 expression in 23 pairs of HCC/paracancerous tissues by quantitative real-time polymerase chain reaction (qRT-PCR) and evaluated HAO2’s expression in The Cancer Genome Atlas (TCGA) database. Furthermore, we examined the biological activity of HAO2 utilizing cell-based functional assays. Additionally, we evaluated the relationship between miR-615-5p and HAO2 in Hep3B cells using a dual-luciferase reporter system and assessed the downstream regulatory mechanisms of miR-615-5p on HAO2. Finally, the nude mice tumor formation experiment was used to determine the impact of HAO2 on the tumorigenicity of HCC cells. Results. HAO2 expression was considerably underexpression in HCC tissues and cells, and patients with low HAO2 expression had poorer disease-free survival. Inhibition of cell proliferation, migration, and invasion was observed when HAO2 was overexpressed. miR-615-5p had a negative relation with HAO2, and miR-615-5p restored HAO2’s biological activity in HCC cells. Additionally, the tumor volume and weight were considerably reduced in the OV-HAO2 group compared to the OV-NC group. Conclusion. HAO2 was found to be underexpressed in HCC tissues and cells, and HAO2 overexpression inhibited HCC cell motility, which was negatively regulated by miR-615-5p. Exogenous expression of HAO2 reduced the tumorigenicity of HCC cells in vivo in nude mice.
Intelligent Tourism Information Service Model considering Tourist Experience in the Environment of Internet of Things
Authors: Liu Han
Published: April 2022

Due to the narrow range of route search and the single choice of the optimal route during the peak period of tourists' tourism, resulting in a long walking time and relatively high route cost, an intelligent tourism information service model considering tourists' experience is proposed in the environment of Internet of things. Firstly, the overall architecture of the Internet of things environment is designed to obtain the characteristic data of the carrying capacity of scenic spots, including tourism resources, psychology, ecology, and economic carrying capacity, based on which the tourist perception experience model is constructed; different service functions such as in-depth mining of Web content, personalized information push, and data information conversion under the environment of Internet of things and the construction of intelligent tourism information service model are realized. The experimental results show that the passenger flow prediction error of the designed model is small and has a good prediction effect of tourist attraction selection demand.
Pathogenesis of Liver Fibrosis and Its TCM Therapeutic Perspectives
Authors: Nan Yang , Su HongChan , Lian XiaoMei , Wu Juan , Liu SuJie , Chen PingPing , Liu ShuMin
Published: April 2022

Liver fibrosis is a pathological process of abnormal tissue proliferation in the liver caused by various pathogenic factors, which will further develop into cirrhosis or even hepatocellular carcinoma if liver injury is not intervened in time. As a diffuse progressive liver disease, its clinical manifestations are mostly excessive deposition of collagen-rich extracellular matrix resulting in scar formation due to liver injury. Hepatic fibrosis can be caused by hepatitis B and C, fatty liver, alcohol, and rare diseases such as hemochromatosis. As the metabolic center of the body, the liver regulates various vital activities. During the development of fibrosis, it is influenced by many other factors in addition to the central event of hepatic stellate cell activation. Currently, with the increasing understanding of TCM, the advantages of TCM with multiple components, pathways, and targets have been demonstrated. In this review, we will describe the factors influencing liver fibrosis, focusing on the effects of cells, intestinal flora, iron death, signaling pathways, autophagy and angiogenesis on liver fibrosis, and the therapeutic effects of herbal medicine on liver fibrosis.
DeepFire: A Novel Dataset and Deep Transfer Learning Benchmark for Forest Fire Detection
Authors: Khan Ali , Hassan Bilal , Khan Somaiya , Ahmed Ramsha , Abuassba Adnan
Published: April 2022

Forest fires pose a potential threat to the ecological and environmental systems and natural resources, impacting human lives. However, automated surveillance system for early forest fire detection can mitigate such calamities and protect the environment. Therefore, we propose a UAV-based forest fire fighting system with integrated artificial intelligence (AI) capabilities for continuous forest surveillance and fire detection. The major contributions of the proposed research are fourfold. Firstly, we explain the detailed working mechanism along with the key steps involved in executing the UAV-based forest fire fighting system. Besides, a robust forest fire detection system requires precise and efficient classification of forest fire imagery against no-fire. Moreover, we have curated a novel dataset (DeepFire) containing diversified real-world forest imagery with and without fire to assist future research in this domain. The DeepFire dataset consists of 1900 colored images with 950 each for fire and no-fire classes. Next, we investigate the performance of various supervised machine learning classifiers for the binary classification problem of detecting forest fire. Furthermore, we propose a VGG19-based transfer learning solution to achieve improved prediction accuracy. We assess and compare the performance of several machine learning approaches such as

k

-nearest neighbors, random forest, naive Bayes, support vector machine, logistic regression, and the proposed approach for accurately identifying fire and no-fire images in the DeepFire dataset. The simulation results demonstrate the efficacy of the proposed approach in terms of accurate classification, where it achieves the mean accuracy of 95% with 95.7% precision and 94.2% recall.
Shared Etiology in Autism Spectrum Disorder and Epilepsy with Functional Disability
Authors: Zahra Aqeela , Wang YunFu , Wang Qun , Wu Jianping
Published: April 2022

Autism spectrum disorders and epilepsies are heterogeneous human disorders that have miscellaneous etiologies and pathophysiology. There is considerable risk of frequent epilepsy in autism that facilitates amplified morbidity and mortality. Several biological pathways appear to be involved in disease progression, including gene transcription regulation, cellular growth, synaptic channel function, and maintenance of synaptic structure. Here, abnormalities in excitatory/inhibitory (E/I) balance ratio are reviewed along with part of an epileptiform activity that may drive both overconnectivity and genetic disorders where autism spectrum disorders and epilepsy frequently co-occur. The most current ideas concerning common etiological and molecular mechanisms for co-occurrence of both autism spectrum disorders and epilepsy are discussed along with the powerful pharmacological therapies that protect the cognition and behavior of patients. Better understanding is necessary to identify a biological mechanism that might lead to possible treatments for these neurological disorders.
A Metadata-Based Multimodal Model for Resource Sharing of British and American Female Literary Works
Authors: Cai Lanlan
Published: April 2022

Based on the perspective of multimodal teaching theory, this study analyzes the establishment of English and American literature appreciation standard course in universities and discusses the necessity of course offering, multimodal teaching implementation, and multi-evaluation of the course, to provide ideas for English literary teaching reform and talent training in universities. In terms of the training of professional talents, to improve their critical thinking and humanistic quality, a British and American literature course in English major study holds a significant place as a mandatory course for English majors. Basic abilities such as an awareness of Western literature and culture are developed into the ability to appreciate and evaluate literary works. This study, based on the premise of standardizing spatial data and sharing data, argues that the traditional data sharing model has flaws and that a sharing mechanism based on metadata management is required. It also proposes a metadata management strategy that can be used as a model for addressing the problem of heterogeneous storage in data sharing.
Damage Mechanism and Wave Attenuation Induced by Blasting in Jointed Rock
Authors: Song Jie-Fang , Lu Cai-Ping , Zhang Xiu-Feng , Guo Ying , Yang Hong-Wei
Published: April 2022

This work uses a combination of simulations performed via numerical models and field observations studied the attenuation of deep-hole blasting stress waves and the evolution mechanism of cracks in a jointed rock mass. First, we conclude that the larger the joint angle is, the larger is the transmission coefficient and smaller is the fractal dimension. Second, the time difference between the peak stress difference and the maximum principal stress on both sides of the blasting hole in the horizontal direction of the rock mass with joints is relatively large, but there is no significant difference in the vertical direction. Finally, an unjointed-rock-mass model and multiple parallel joint model are established to explore the attenuation of stress waves and damage effect of multiple joint rock mass, it is concluded that the larger the angle, the smaller is the particle peak velocity and amplitude attenuation, and as the number of stress waves passing through the joints increases, the amplitude gradually decreases and the high-frequency amplitude decreases more significantly than the low-frequency amplitude. The research conclusions of this paper further reveal the damage mechanism induced by a blasting stress wave on jointed rock masses and the law of stress wave propagation and attenuation.
Construction of Surface Water Pollution Prediction Model Based on Machine Learning
Authors: Che Yuanhong , Wei Zhangdong , Wen Jing
Published: April 2022

Existing prediction models have low prediction accuracy for surface water pollution with many influencing factors. Taking algal bloom prediction as the entry point of surface water pollution research, LASSO-LARS algorithm is adopted to select the main factors affecting algal bloom variables. At the same time, combined with BP neural network in machine learning, a surface water pollution prediction model based on machine learning is proposed. The results show that the proposed method can accurately predict the algal blooms by using the BP neural network algal bloom prediction model, where the LASSO-LARS algorithm is used to select the variables, such as water temperature, pH, transparency, conductivity, dissolved oxygen, ammonia nitrogen, and chlorophyll a, as model inputs, and the relative error of prediction is less than 5.2%. Thus, the proposed method has certain validity and practical application value.
Study of the Characteristics Mechanical Damage and Constitutive Model of Crushed-Rocks from High-Grade Highway in Permafrost Region
Authors: Zhang Qinshuai , Wang Qingzhi , Fang Jianhong , Zhao Xiangqing , Li Jizhen , Li Shuan
Published: April 2022

Three types of rocks are selected to conduct uniaxial compression and microscope experimental to study the influences of freeze-thaw cycles on crushed-rocks from Gonghe-Yushu high-graded highway, which is the first high-grade highway built in permafrost regions in China. Using the hypothesis of Lemaitre’s strain equivalence principle and a theoretical model of crushed-rock deterioration after freeze-thaw cycles was established and verified by the experimental data. The experimental results showed that with an increase of the number of freeze-thaw cycles, the microcracks of saturated red sandstone increased, and this phenomenon is not obvious on other rocks. Moreover, different degrees of spalling and cracks appeared, with common physical parameters gradually decreasing. The uniaxial compressive strength of rock decreases exponentially with the increase of the number of freeze-thaw cycles. The mechanism of the influence of freeze-thaw cycles on the fine structure of the three different crushed-rocks were then analyzed using SEM scanning. Freeze-thaw cycles weaken the connection between rock mineral particles and increase the surface micropores. The damage model of freeze-thaw and load can well describe the damage law of crushed-rocks.
Classification of EEG Signal-Based Encephalon Magnetic Signs for Identification of Epilepsy-Based Neurological Disorder
Authors: Kaur Arshpreet , Gupta Suneet , Kathiravan M. , Nasrullah Syed , Paul Chayan , Rahin Saima Ahmed
Published: April 2022

Magnetoencephalography (MEG) is now widely used in clinical examinations and medical research in many fields. Resting-state magnetoencephalography-based brain network analysis can be used to study the physiological or pathological mechanisms of the brain. Furthermore, magnetoencephalography analysis has a significant reference value for the diagnosis of epilepsy. The scope of the proposed research is that this research demonstrates how to locate spikes in the phase locking functional brain connectivity network of the Desikan-Killiany brain region division using a neural network approach. It also improves detection accuracy and reduces missed and false detection rates. The automatic classification of epilepsy encephalomagnetic signals can make timely judgments on the patient’s condition, which is of tremendous clinical significance. The existing literature’s research on the automatic type of epilepsy EEG signals is relatively sufficient, but the research on epilepsy EEG signals is relatively weak. A full-band machine learning automatic discrimination method of epilepsy brain magnetic spikes based on the brain functional connection network is proposed. The four classifiers are comprehensively compared. The classifier with the best effect is selected, and the discrimination accuracy can reach 93.8%. Therefore, this method has a good application prospect in automatically identifying and labeling epileptic spikes in magnetoencephalography.
Analysis of Public Management Performance Evaluation Model Based on Fuzzy DEA Model
Authors: Sun Chenguang , Zhang Le , Mao Zhihui
Published: April 2022

The evaluation of public management performance is the key to identify the effectiveness of the work of public administration, and public policy is an important guarantee for the orderly promotion of the work of public administration; therefore, it is important to analyze the effectiveness of public management policies to evaluate public management performance. Taking environmental protection policies as an example, this paper constructs an environmental‐economic DEA efficiency analysis model using the DEA method to conduct a comprehensive evaluation of EEE in different provinces of China. It is found that EEE is highly correlated with the regional economic development level and is highly influenced by policy fluctuations, but the overall EEE of different provinces from 2016 to 2020 tends to be consistent and has a certain degree of stability. The conclusions of this paper have important implications for policy guidance, emphasizing the need to focus on the coordination of economic development and environmental protection, and the need for the improvement of the environmental policy system to be continuously optimized in an incremental manner, taking into account the actual situation of local development.
Association between Immunohistochemistry Markers and Tumor Features and Their Diagnostic and Prognostic Values in Intrahepatic Cholangiocarcinoma
Authors: He Jiannan , Zhang Cao , Shi Qinye , Bao Fangping , Pan Xiang , Kuai Yue , Wu Jingjin , Li Li , Chen Ping , Huang Yian , Xu Jianhong
Published: April 2022

This study investigated the expression of some frequently used immunohistochemistry (IHC) markers. Besides, we evaluated their correlations with the clinical features and outcomes of intrahepatic cholangiocarcinoma (ICC). Patients who underwent surgical removal of the ICC tumors were followed up for 4 years. The paraffin-embedded sections were used to obtain different markers, including CK7, CK19, CK20, CDX2, Glypican3, Hepa1, Ki-67, Villin, and SATB1. Overall survival in relation to IHC marker expression patterns and other clinical characteristics was evaluated by Kaplan-Meier survival curve and log-rank test, followed by the Cox proportional hazard model (to evaluate the relationship between multiple factors and the overall postoperative survival). A total of 122 ICC patients (67 males and 55 females, averagely aged 57.75) were included in this study. There were 44 cases with vascular invasion, 46 cases with lymphatic metastasis, and 13 cases with distant metastasis. CK7 was negatively correlated with lymphatic metastasis; and in distant-metastasis cases, the positive ratio of SATB1 was lower. Interestingly, SATB1 expression indicated a poorer survival, while Villin expression was associated with a better survival. The COX regression analysis showed that female was a protective factor versus male, Villin expression was a strong protective factor, and Ki-67 expression was correlated with a poor survival. Together, IHC markers are associated with tumor features and postoperative survival, especially for SATB1 as a risk factor and Villin as a protective marker, and female ICC patients may have better survival than males.
Image Retrieval Technology of Smart Archives from the Perspective of National Reading
Authors: Danqiu Qu , Hao Ren
Published: April 2022

In order to improve the image retrieval effect of smart archives, reduce retrieval time, and enhance retrieval performance, this paper proposes an image retrieval technology for smart archives from the perspective of national reading. According to the concept of nationwide reading and considering the internal and external influencing factors, mobile visual retrieval service for users of smart archives is provided, and the mobile visual retrieval model for users of smart archives is constructed by using a standardized structure and mixed structure. During the operation of the model, the image feature similarity of the wisdom archive is calculated, and the image is processed by the corresponding module, so as to realize the image retrieval technology research of the wisdom archive from the national reading perspective. The experimental results show that the designed technology has better retrieval effect, shorter time, and better performance.
HNRNPA2B1 Demonstrates Diagnostic and Prognostic Values Based on Pan-Cancer Analyses
Authors: Chen Chaoyang , Huang Lipeng , Sun Qingyu , Yu Zhichen , Wang Xiaoyan , Bu Liang
Published: April 2022

Some studies have suggested heterogeneous nuclear ribonucleoprotein A2/B1 (HNRNPA2B1) to be a promoter in cancer development. Nonetheless, no detailed pan-cancer investigation has been reported. Thus, this study explored the possible oncogenic role of HNRNPA2B1, such as its expression levels, gene alteration, protein–protein interaction network, immune infiltration, and prognostic value in different cancer types using The Cancer Genome Atlas web platform. Many types of cancer exhibit HNRNPA2B1 overexpression, which is notably associated with poor prognosis. We also found that HNRNPA2B1 with different methylation levels causes a varied prognosis in lung adenocarcinoma (LUAD). It is noteworthy that HNRNPA2B1 levels are connected with cancer-associated fibroblasts in cancers, such as adrenocortical carcinoma, LUAD, and stomach adenocarcinoma. In addition, HNRNPA2B1 participates in the spliceosome- and cell cycle-associated pathways. Finally, HNRNPA2B1 is highly valued in the diagnosis of LUAD, lung squamous cell carcinoma, breast invasive carcinoma, esophageal carcinoma, and liver hepatocellular carcinoma. This systematic study highlighted the role of HNRNPA2B1 in pan-cancer progression.
Heavy Metals Contamination and Water Quality Parameter Conditions in Jatiluhur Reservoir, West Java, Indonesia
Authors: Prayoga Gatot , Utomo Bagus Amalrullah , Effendi Hefni
Published: April 2022

Waste pollution into the Citarum River (main water source of Jatiluhur Reservoir) was dominated by the manufacturing industry sector (textile, chemical, metal, pharmaceutical). Industrial was the most common contributor to heavy metal waste. Heavy metal contamination into waters will cause any problems, one of which was the emergence of various diseases both short and long term. Based on the issues, the study of heavy metal contamination and also water quality parameter conditions in the Jatiluhur Reservoir was necessary. The heavy metals (Cu, Zn, Hg, Pb, Cd) contents were determined using the X-Ray Fluorescence (XRF) Spectrometry method (for sediment) and Atomic Absorption Spectrometry (AAS) method (for water). The other water quality parameters were analyzed using the methods from the Indonesian National Standard (SNI). Furthermore, the data were compared to the Canadian Sediment Quality Guidelines (for heavy metal in sediment) and water quality standards from Government Regulation of the Republic of Indonesia Number 82 of 2001 (Class 3) (for water quality parameters). Concerning the discussion, Jatiluhur Reservoir was divided into three zones i.e. the inlet area, main inundation area, and outlet area. Conditions in the sedimentary layer, mercury (Hg) have accumulated throughout the Jatiluhur Reservoir area with conditions exceeding the maximum limit, while Cu metal tends to accumulate in the inlet area with conditions exceeding the minimum limit. For other heavy metals, exceed the minimum limit at some locations, but more results were below that. Although all heavy metals have not been detected in water, this was a warning that the presence of heavy metals in sediments can potentially dissolve into the water, the most extreme thing that can happen was upwelling. If this happens, the heavy metals can be contained excessively in water, harmful to and possibly consumed by aquatic biota and human. Considering these conditions, the biota that was most likely to be exposed was benthic organisms. In general, the water quality parameters in Jatiluhur Reservoir meet the quality standards. Only ammonia that does not meet the quality standards for sensitive fish life, regarding the massively of aquaculture activity in this reservoir.
NEUSTON DIVERSITY AND DENSITY AS BIOINDICATOR FOR WATER QUALITY
Authors: Nurza Imam Safir Alwan , Vania Jesslyn Gitta , Reynaldi Muhammad Khatami , Rasyid Zaki Gunawan , Komala Ratna
Published: April 2022

Lakes and waterfalls are freshwater ecosystems having important roles in ecology, tourism and economic aspects.  Among living organisms existing in lakes and waterfalls is neuston.  Neuston lives on the surface and below the surface of the waters.  Neuston can be used as a bioindicator for water quality due to the neuston’s high level of sensitivity toward pollutants. The purpose of this study was to determine the density and diversity of neuston as a bioindicator for water quality in the lake and waterfall. The methods used were survey and observation. The study was carried out by using purposive sampling at two locations, namely the lake and waterfall of Situ Gunung, Sukabumi, with a sampling area of    1 x 1 m2. The samples obtained were put into bottles containing 70% alcohol to be identified in the laboratory. Environmental parameters measured were air and water temperature, water pH, water depth, turbidity, water flow velocity, dissolved oxygen, substrate and weather conditions. The results obtained indicated that the lake and waterfall of Situ Gunung, Sukabumi had highest neuston densities were shown by Gerris lacustris and Dineutus assimilis. The lowest neuston densities were shown by Metrobates hesperius, Gerris comatus, Aquarius remiges and Trepobates pictus. This study showed that the environmental parameters of the lake and waterfall of Situ Gunung, Sukabumi can still support the survival of the existing neustons.
A Reflexão no último livro de Giannotti
Authors: Figueiredo Vinicius Berlendis de
Published: April 2022

Em seu último livro, Heidegger/Wittgenstein — Confrontos (2020), José Arthur Giannotti conclui sua apropriação filosófica da obra de L. Wittgenstein, iniciada com A apresentação do mundo (1995). Examino neste artigo de que modo o corpo a corpo com o filósofo austríaco fez Giannotti rever questões com que se ocupara ao longo de seus trabalhos sobre Marx, em especial no que diz respeito à questão pelo estatuto do lógos prático e da Reflexão.
O lugar da intersubjetividade no Sistema do idealismo transcendental de Schelling
Authors: Cunha João Geraldo Martins da
Published: April 2022

Neste trabalho, pretendo oferecer uma interpretação da “Segunda proposição” da filosofia prática — Capítulo IV do Sistema do idealismo transcendental (1800). A partir dessa delimitação textual, minha hipótese não vai além de uma sugestão segundo a qual a relação de interação recíproca e indireta entre as autoconsciências ocupa um “lugar” estratégico no argumento de Schelling, uma vez que, através dela, pelo menos em princípio, dois problemas seriam resolvidos: (1) aquele acerca da contradição enraizada no limite originário dos atos transcendentais de autoconstituição da inteligência como consciência individual e livre; (2) e aquele acerca da articulação entre filosofia teórica e filosofia prática.
DOCK4 Regulation of Rho GTPases Mediates Pulmonary Vascular Barrier Function
Authors: Yazbeck Pascal , Cullere Xavier , Bennett Paul , Yajnik Vijay , Wang Huan , Kawada Kenji , Davis Vanessa M. , Parikh Asit , Kuo Andrew , Mysore Vijayashree , Hla Timothy , Milstone David S. , Mayadas Tanya N.
Published: April 2022

Background:

The vascular endothelium maintains tissue-fluid homeostasis by controlling the passage of large molecules and fluid between the blood and interstitial space. The interaction of catenins and the actin cytoskeleton with VE-cadherin (vascular endothelial cadherin) is the primary mechanism for stabilizing AJs (adherens junctions), thereby preventing lung vascular barrier disruption. Members of the Rho (Ras homology) family of GTPases and conventional GEFs (guanine exchange factors) of these GTPases have been demonstrated to play important roles in regulating endothelial permeability. Here, we evaluated the role of DOCK4 (dedicator of cytokinesis 4)—an unconventional Rho family GTPase GEF in vascular function.

Methods:

We generated mice deficient in DOCK4‚ used DOCK4 silencing and reconstitution approaches in human pulmonary artery endothelial cells‚ used assays to evaluate protein localization, endothelial cell permeability, and small GTPase activation.

Results:

Our data show that DOCK4-deficient mice are viable. However, these mice have hemorrhage selectively in the lung, incomplete smooth muscle cell coverage in pulmonary vessels, increased basal microvascular permeability, and impaired response to S1P (sphingosine-1-phosphate)–induced reversal of thrombin-induced permeability. Consistent with this, DOCK4 rapidly translocates to the cell periphery and associates with the detergent-insoluble fraction following S1P treatment, and its absence prevents S1P-induced Rac-1 activation and enhancement of barrier function. Moreover, DOCK4-silenced pulmonary artery endothelial cells exhibit enhanced basal permeability in vitro that is associated with enhanced Rho GTPase activation.

Conclusions:

Our findings indicate that DOCK4 maintains AJs necessary for lung vascular barrier function by establishing the normal balance between RhoA (Ras homolog family member A) and Rac-1–mediated actin cytoskeleton remodeling, a previously unappreciated function for the atypical GEF family of molecules. Our studies also identify S1P as a potential upstream regulator of DOCK4 activity.
Abstract 12125: Baseline Analysis of National Malaysian Heart Failure Registry Indicates Hospitalization as a Key Opportunity to Initiate GDMT in HF Patients
Authors: Wan Ahmad Wan Azman , SK Abdul Kader Muhamad Ali S , Ross Noel Thomas , Ramli Ahmad Wazi , Ghazi Azmee Mohd , Che Hassan Hamat Hamdi , Lee Chuey Yan , Fegade Mayuresh , Zainal Abidin Hafisyatul Aiza , Karthikesan Dharmaraj , Mohd Amin Nor Hanim , Ong Tiong Kiam , Mahendran Kauthaman A , Muthusamy Tamil Selvan , ghapar drkahar ,   MYHF Investigators
Published: April 2022

Introduction:
There is scarcity of big data on Heart Failure (HF) in the Asean region in the current era of guideline directed medical therapy (GDMT). Malaysia Heart Failure (MYHF) Registry was set up in 2019 to advance fundamental understanding of the burden, morbidity and mortality among Malaysian patients with HF

Objective:
This analysis is to determine baseline characteristics, diagnostic work up and treatment pattern of patients admitted with the diagnosis of HF to address the unmet and growing clinical needs of HF patients in Malaysia.

Methods:
The first ever National HF Registry is planned as a prospective, observational study of HF patients (NYHA II-IV) across 18 tertiary care centers in Malaysia over a period of 3 years starting in 2019. This Registry recruited all patients admitted with a clinical diagnosis of HF by the attending physician with a minimum hospital stay of 24 hours for stabilisation prior to discharge. Primary endpoint is all cause mortality and hospital readmission for acute decompensated heart failure (ADHF) at one year. This paper summarizes baseline and clinical characteristics of patients along with treatment pattern and outcomes of index hospitalization.

Results:
We evaluated 2673 patients with mean age of 60 years (SD 13.8). De novo HF constituted one third of hospitalizations. Mean left ventricular ejection fraction (LVEF) was 36.4% (SD 15.3) stratified into 65% of HFrEF (LVEF ≤ 40%), 21% of HFpEF (LVEF ≥ 50%); and 11% of HFmrEF (LVEF 41-49%). Prior to index HF hospitalization, proportion of patients on BB, ACEi, ARBs, ARNI, MRA and ivabradine were 57%, 32%, 8%, 6%, 24% & 6% respectively changing to 79%, 36%, 8%, 15%, 43% & 12% at discharge of the patient. Mean length of hospital stay was 7.3 days (SD 8.9). In-hospital mortality was 3% attributed largely to cardiac causes (68%).

Conclusions:
The registry identifies at-risk patient populations with HF and highlights lesser prevalence of HFpEF compared to other HF registries. The analysis shows increased usage of GDMT post HF hospitalization event remarkably for BB, MRA, ivabradine and ARNI indicating hospitalization as a key opportunity to initiate GDMT in HF patients.
Abstract 12152: The Association of Improvement in Left Ventricular Ejection Fraction With Outcomes in Patients With Heart Failure With Reduced Ejection Fraction - Data From CHAMP-HF
Authors: Devore Adam D , Hellkamp Anne , Thomas Laine E , Albert Nancy M , Butler Javed , Patterson J Herbert H , Spertus John , Williams Fredonia , Shen Xian , Hernandez Adrian F , Fonarow Gregg C
Published: April 2022

Background:
Among patients with heart failure with reduced ejection fraction (HFrEF), improvements in left ventricular EF (LVEF) are associated with better outcomes in select populations. How changes in LVEF are associated with outcomes in routine clinical practice is not clearly defined.

Methods:
CHAMP-HF was a prospective registry of US outpatients with HF and an LVEF
<
40%. Enrolled patients completed the Kansas City Cardiomyopathy Questionnaire 12-item questionnaire (KCCQ-12) at regular intervals and were followed as part of routine care for assessments of LVEF and clinical outcomes. We analyzed all patients with baseline and
>
1 LVEF assessment during follow-up, and we assessed for associations between improvements in LVEF (
>
5%) over time and concurrent changes in KCCQ-12 and subsequent risk of HF hospitalization or all-cause death.

Results:
Among patients enrolled in CHAMP-HF, 2092 had a baseline and follow-up LVEF assessment. The median age was 67 years (25
th
, 75
th
percentile 58, 75), 29% were female, 64% had concomitant coronary disease, and the median duration of HF was 2.7 years (0.6, 6.8). The median baseline LVEF was 30% (23, 35) and 1032 (49%) patients had a
>
5% improvement in LVEF over time. Patients that had an improvement in LVEF over time had a significant simultaneous improvement in KCCQ-12 overall summary score compared with patients with no improvement (change from baseline: +6.8 vs +3.0, adjusted effect estimate 3.8 [95% CI 1.8 to 5.7]) (
Table
). Similarly, subsequent HF hospitalization or all-cause death occurred in 14% in the LVEF improvement group vs 27% in the no LVEF improvement group (adjusted hazard ratio 0.52, 95% CI 0.43 to 0.63).

Conclusions:
In a large cohort of outpatients with chronic HFrEF, improvements in LVEF were common and associated with improvements in health status and clinical outcomes. These data underscore the importance of changes in LVEF as a treatment target for medical interventions for patients with chronic HFrEF.
Abstract 12156: Loss of Thiol on C674 SERCA2 Leads to Ca2
+
Mishandling With Hypertrophy in Right Ventricular Exposed to Hypoxia
Authors: Kagami Kazuki , Ito Kei , Yada Hirotaka , Yumita Yusuke , Iwashita Midori , Yuki Ishinoda , Toya Takumi , Namba Takayuki , Ikegami Yukinori , Yasuda Risako , Masaki Nobuyuki , Ido Yasuo , Nagatomo Yuji , Adachi Takeshi
Published: April 2022

Introduction:
Right ventricular (RV) dysfunction is the strongest predictor of mortality in pulmonary artery hypertension (PAH). Reactive oxygen species in heart failure cause irreversible oxidation of sarcoplasmic reticulum (SR) Ca
2+
-ATPase2 (SERCA2) C674, which results in SERCA2 dysfunction and intracellular Ca
2+
overload. However, the contribution of the loss of the thiol on C674 SERCA2 and RV failure in PAH remains unclear.

Hypothesis:
The loss of the thiol on C674 SERCA2 contributes to the RV dysfunction in PAH.

Methods & Results:
We employed the SERCA2 C674S heterozygote knock-in (SKI) mice, in which C674 is replaced by serine, mimics oxidative modification of SERCA2 by loss of the specific thiol. Wild-type mice (WT) and SKI were exposed to either normoxia (Nx) or chronic hypoxia (Hx) for four weeks. Hypoxia elevated RV systolic pressure (WT-Nx: 21.4mmHg vs. WT-Hx: 37.0mmHg, P<0.0001) and represented pulmonary vascular remodeling similarly both in WT and SKI. In histology, SKI-Hx showed the markedly increased Fulton's Index with higher levels of the mRNA expression of atrial natriuretic peptide and B-type natriuretic peptide than WT-Hx. In the exercise test, exercise duration was shorter and peak oxygen consumption was decreased in SKI-Hx than WT Hx. The mRNA levels of SERCA2 were not changed among the four groups. In Ca
2+
transient measurement of isolated myocytes with Fluo-4, SKI prolonged the time to 50% of cytosolic Ca
2+
extrusion (T50) and lower the peak Ca
2+
transient amplitude (F/F0) than WT under Nx. Prolonged T50 and lowered peak F/F0 were observed in WT-Hx, both of which additionally progressed in SKI-Hx.

Conclusions:
Hypoxia impaired SR Ca
2+
homeostasis in RV. SKI additionally impaired SR Ca
2+
homeostasis with the progression of RV hypertrophy. The loss of the thiol on C674 SERCA2 contributed to the pathogenesis of RV failure in PAH.
Abstract 12195: Racial and Ethnic Disparities in Reperfusion Interventions and Stroke Center Care in California
Authors: Zachrison Kori S , Samuels-Kalow Margaret , Li Sijia , Reeves Mathew J , Hsia Renee Y , Schwamm Lee H , Camargo Carlos A
Published: April 2022

Background:
There is significant hospital-level variation in quality of acute stroke care delivery. As a result, patients’ access to disability-reducing reperfusion interventions and stroke center care depends on the initial hospital of presentation and interhospital transfer. Our objective was to examine racial and ethnic disparities in access to reperfusion interventions, and whether interhospital patient transfer mitigates disparities in access to stroke center care.

Methods:
Using statewide administrating data including all emergency department and hospital discharges in California from 2010-2017, we identified acute ischemic stroke patients and outcomes of interest: thrombolytic therapy, endovascular thrombectomy (EVT) receipt, interhospital transfer, and discharge from primary or comprehensive stroke center hospital. We used hierarchical logistic regression modeling to identify the relationship between race/ethnicity and outcomes after accounting for important patient- and hospital-level factors.

Results:
Of 336,247 ischemic stroke patient encounters during the study period, 186,444 (55.4%) were non-Hispanic white, 66,016 (19.6%) Hispanic, 34,596 (10.3%) non-Hispanic Black and 35,784 (10.6%) non-Hispanic Asian/Pacific Islander. Relative to non-Hispanic white patients, adjusted odds of thrombolytic receipt were lower for Hispanic, non-Hispanic Asian/Pacific Islander, and non-Hispanic Black patients (Figure). Adjusted odds of EVT were lower for Hispanic and non-Hispanic Black patients. Adjusted odds of interhospital transfer were lower among Hispanic and non-Hispanic Asian patients. Adjusted odds of discharge from a stroke center hospital was similar across racial/ethnic groups.

Conclusions:
There are racial/ethnic disparities in receipt of reperfusion interventions among stroke patients in California. Configuration of the stroke system of care, hospital resources, and transfer patterns may contribute.
Abstract 12229: Left Ventricular Non-Compaction in Childhood: Echocardiographic Follow-Up and Prevalence in First-Degree Relatives
Authors: Kock Thilde Olivia , Boerresen Marie F , Sillesen Anne-sophie , Norsk Jakob B , Paerregaard Maria Munk M , Vejlstrup Niels G , Christensen Alex , Iversen Kasper , Bundgaard Henning , Axelsson Raja Anna
Published: April 2022

Introduction:
Left ventricular non-compaction (LVNC) is characterized by excessive trabeculations of the left ventricular wall and may be associated with reduced systolic function. It is debated whether LVNC is congenital or may develop later as part of other cardiomyopathies. The clinical importance and heredity of LVNC with normal systolic function is unclear.

We aimed to describe the cardiac development in children with LVNC from birth to 2-4 years of age, compared to matched controls. Additionally, we aimed to describe the prevalence of LVNC in first-degree relatives.

Methods:
A follow-up transthoracic echocardiography was performed in children at 2-4 years of age diagnosed with LVNC at birth (<30 days) in the Copenhagen Baby Heart Study. Cases were matched to controls on mothers age at delivery, parity, and age of child at follow-up. First-degree relatives (parents, siblings and half-siblings) were also included. LVNC was defined as a non-compact to compact ratio of myocardium of ≥2 in at least one left ventricular segment, measured in 24 segments in end-diastole perpendicular to the left ventricular cavity as previously suggested.

Results:
Of the 16 children diagnosed with LVNC at birth, 10 have been reevaluated (age 3.5 (interquartile range (IQR) 3, 4) years, 80% male) together with 20 matched controls (age 4 (IQR 3, 4) years, 70% male), 29 first-degree relatives in case group (age 29 (IQR 4, 35) years, 45% male) and 55 first-degree relatives in control group (age 32 (IQR 11, 36) years, 51% male). In probands, the extent of trabeculation (13% vs. 12%, p=0.97) and fractional shortening (FS) (29% vs. 31%, p=0.24) were unchanged from birth to follow-up. At follow-up, the median left ventricular FS was significantly lower in probands compared to matched controls (31% vs. 33%, p=0.03). Ten (35%) first-degree relatives to probands fulfilled criteria for LVNC compared to 0 (0%) of first-degree relatives to controls (p<0.001).

Conclusions:
Children with LVNC diagnosed neonatally as part of a population study had no further progression of left ventricular dysfunction or extent of trabeculation at the age of 2-4 years, but systolic function was reduced compared to matched controls. One third of first-degree relatives to children with LVNC fulfilled criteria for LVNC.
Abstract 12236: Population-Level Analyses of Alcohol Consumption as a Predictor of Acute Atrial Fibrillation Episodes
Authors: Aung Sidney , nah gregory , Vittinghoff Eric , Groh Christopher , Fang Christina , Marcus Gregory M
Published: April 2022

Introduction:
We conducted an instrumental variable analysis to test whether acute alcohol consumption leads to increased emergency department (ED) visits for AF at the level of the general population.

Methods:
We analyzed blood alcohol concentration measurements obtained from individuals using commercially available Bluetooth-enabled breathalyzer devices (BACtrack, San Francisco, CA) collected between January 1, 2014 and December 31, 2016 to identify dates associated with heightened alcohol consumption that corresponded to recurrent, nationally-recognized events. Outcomes included ED visits with AF (main analysis) or supraventricular tachycardia (SVT; as a negative control) in California between January 1, 2005 to December 30, 2015 using Office of Statewide Health Planning and Development data. A secondary analysis examining rates of new-onset AF was conducted. We utilized population denominators for age, sex, and race/ ethnicity in California from the US Census. Multivariable adjusted Poisson regression models were utilized to compare rates of AF during and 6 days following instrumental events compared to all other days of the year.

Results:
Using 1,269,054 breath alcohol measurements obtained from 36,158 individuals, we identified 8 recurring events associated with heightened alcohol consumption. After multivariable adjustment, excess alcohol consumption inferred from our instrumental variables was associated with 719 additional AF visits per 100,000 person-years (95% CI 189-1249, p = 0.008;
Table
). The association between these events and new-onset AF exhibited a stronger effect (1,757 additional visits, 95% CI 945-2,569, p < 0.001). A negative control analysis failed to exhibit significantly heightened rates of SVT during those same dates.

Conclusion:
These data suggest that acute alcohol consumption is associated with a higher risk for discrete AF episodes, as well as for new-onset (incident) AF, in the general population.