bluuebunny/crossref_metadata_2025 huggingface dataset:
The Health Costs of Revised Coal Mining Limits in Northern Bohemia
Authors: Máca Vojtěch , Melichar Jan
Published: January 2016

Brown coal has been the major source of energy for more than 150 years for the Czech economy, but its role in the coming decades is nowadays the source of heated debate. Many of the recurring discussions address the coal reserves that were set aside in 1991 in order to halt the massive destruction of the landscape and the unprecedented deterioration of the human environment in Northern Bohemia. We study the proposed variants of the revised mining limits using the impact-pathway approach in order to quantify and monetize the health effects of airborne emissions from coal mining and the use of extracted coal for the production of electricity and heat. We find that the dominant health impacts associated with the revision of the limits, estimated to be up to 7 billion euros over the 2015–2050 period, will stem from the use of coal for the production of electricity and heat and that a large part of the impact will be borne by populations outside the Czech Republic.
Implementing a Novel Hybrid Maximum Power Point Tracking Technique in DSP via Simulink/MATLAB under Partially Shaded Conditions
Authors: Hajighorbani Shahrooz , Mohd Radzi Mohd , Ab Kadir Mohd , Shafie Suhaidi , Mohd Zainuri Muhammad
Published: January 2016

This paper presents a hybrid maximum power point tracking (MPPT) method to detect the global maximum power point (GMPP) under partially shaded conditions (PSCs), which have more complex characteristics with multiple peak power points. The hybrid method can track the GMPP when a partial shadow occurs either before or after acquiring the MPP under uniform conditions. When PS occurs after obtaining the MPP during uniform conditions, the new operating point should be specified by the modified linear function, which reduces the searching zone of the GMPP and has a significant effect on reducing the reaching time of the GMPP. Simultaneously, the possible MPPs are scanned and stored when shifting the operating point to a new reference voltage. Finally, after determining the possible location of the GMPP, the GMPP is obtained using the modified P&O. Conversely, when PS occurs before obtaining the MPP, the referenced MPP should be specified. Thus, after recognizing the possible location of the GMPP, the modified P&O can be used to obtain the GMPP. The simulation and experimental implementations for the proposed algorithm are performed with different scenarios of shadowing under different irradiations, which clearly indicate that the proposed method is robust and has a fast tracking speed. Moreover, this work presents the load sizing method for PSCs to avoid controller failure when detecting the GMPP. Additionally, in this paper, the user-friendly method for programming the digital signal processing (DSP) via Simulink/MATLAB is presented in detail.
Electric Vehicles in Logistics and Transportation: A Survey on Emerging Environmental, Strategic, and Operational Challenges
Authors: Juan Angel , Mendez Carlos , Faulin Javier , De Armas Jesica , Grasman Scott
Published: January 2016

Current logistics and transportation (L&T) systems include heterogeneous fleets consisting of common internal combustion engine vehicles as well as other types of vehicles using “green” technologies, e.g., plug-in hybrid electric vehicles and electric vehicles (EVs). However, the incorporation of EVs in L&T activities also raise some additional challenges from the strategic, planning, and operational perspectives. For instance, smart cities are required to provide recharge stations for electric-based vehicles, meaning that investment decisions need to be made about the number, location, and capacity of these stations. Similarly, the limited driving-range capabilities of EVs, which are restricted by the amount of electricity stored in their batteries, impose non-trivial additional constraints when designing efficient distribution routes. Accordingly, this paper identifies and reviews several open research challenges related to the introduction of EVs in L&T activities, including: (a) environmental-related issues; and (b) strategic, planning and operational issues associated with “standard” EVs and with hydrogen-based EVs. The paper also analyzes how the introduction of EVs in L&T systems generates new variants of the well-known Vehicle Routing Problem, one of the most studied optimization problems in the L&T field, and proposes the use of metaheuristics and simheuristics as the most efficient way to deal with these complex optimization problems.
Impacts of Forest to Urban Land Conversion and ENSO Phase on Water Quality of a Public Water Supply Reservoir
Authors: Elias Emile , Rodriguez Hugo , Srivastava Puneet , Dougherty Mark , James Darren , Smith Ryann
Published: January 2016

We used coupled watershed and reservoir models to evaluate the impacts of deforestation and l Niño Southern Oscillation (ENSO) phase on drinking water quality. Source water total organic carbon (TOC) is especially important due to the potential for production of carcinogenic disinfection byproducts (DBPs). The Environmental Fluid Dynamics Code (EFDC) reservoir model is used to evaluate the difference between daily pre- and post- urbanization nutrients and TOC concentration. Post-disturbance (future) reservoir total nitrogen (TN), total phosphorus (TP), TOC and chlorophyll-a concentrations were found to be higher than pre-urbanization (base) concentrations (p < 0.05). Predicted future median TOC concentration was 1.1 mg·L−1 (41% higher than base TOC concentration) at the source water intake. Simulations show that prior to urbanization, additional water treatment was necessary on 47% of the days between May and October. However, following simulated urbanization, additional drinking water treatment might be continuously necessary between May and October. One of six ENSO indices is weakly negatively correlated with the measured reservoir TOC indicating there may be higher TOC concentrations in times of lower streamflow (La Niña). There is a positive significant correlation between simulated TN and TP concentrations with ENSO suggesting higher concentrations during El Niño.
Experiment-Based Sensitivity Analysis of Scaled Carbon-Fiber-Reinforced Elastomeric Isolators in Bonded Applications
Authors: Hedayati Dezfuli Farshad , Alam M.
Published: January 2016

Fiber-reinforced elastomeric isolators (FREIs) are a new type of elastomeric base isolation systems. Producing FREIs in the form of long laminated pads and cutting them to the required size significantly reduces the time and cost of the manufacturing process. Due to the lack of adequate information on the performance of FREIs in bonded applications, the goal of this study is to assess the performance sensitivity of 1/4-scale carbon-FREIs based on the experimental tests. The scaled carbon-FREIs are manufactured using a fast cold-vulcanization process. The effect of several factors including the vertical pressure, the lateral cyclic rate, the number of rubber layers, and the thickness of carbon fiber-reinforced layers are explored on the cyclic behavior of rubber bearings. Results show that the effect of vertical pressure on the lateral response of base isolators is negligible. However, decreasing the cyclic loading rate increases the lateral flexibility and the damping capacity. Additionally, carbon fiber-reinforced layers can be considered as a minor source of energy dissipation.
Environmental Change in the Agro-Pastoral Transitional Zone, Northern China: Patterns, Drivers, and Implications
Authors: Jiang Chong , Wang Fei
Published: January 2016

Chengde city is located in the agro–pastoral transitional zone in northern China near the capital city of Beijing, which has experienced large-scale ecological construction in the past three decades. This study quantitatively assessed the environmental changes in Chengde through observation records of water resources, water environment, atmospheric environment, and vegetation activity and investigated the possible causes. From the late 1950s to 2002, the streamflow presented a downward trend induced by climate variability and human activities, with contribution ratios of 33.2% and 66.8%, respectively. During 2001–2012, the days of levels I and II air quality presented clear upward trends. Moreover, the air pollutant concentration was relatively low compared with that in the adjacent areas, which means the air quality has improved more than that in the neighboring areas. The water quality, which deteriorated during 1993–2000, began to improve in 2002. The air and water quality changes were closely related to pollutant emissions induced by anthropogenic activities. During 1982–2012, the vegetation in the southeastern and central regions presented restoration trends, whereas that in the northwestern area showed degradation trends. The pixels with obvious degradation trends correlated significantly with annual mean temperature and annual precipitation. Ecological engineering also played a positive role in vegetation restoration. This analysis can be beneficial to environment managers in the active response and adaptation to the possible effects of future climate change, population growth, and industrial development and can be used to ensure sustainable development and environmental safety.
Seasonal Shifts in Primary Water Source Type: A Comparison of Largely Pastoral Communities in Uganda and Tanzania
Authors: Pearson Amber , Zwickle Adam , Namanya Judith , Rzotkiewicz Amanda , Mwita Emiliana
Published: January 2016

Many water-related illnesses show an increase during the wet season. This is often due to fecal contamination from runoff, yet, it is unknown whether seasonal changes in water availability may also play a role in increased illness via changes in the type of primary water source used by households. Very little is known about the dynamic aspects of access to water and changes in source type across seasons, particularly in semi-arid regions with annual water scarcity. The research questions in this study were: (1) To what degree do households in Uganda (UG) and Tanzania (TZ) change primary water source type between wet and dry seasons?; and (2) How might seasonal changes relate to water quality and health? Using spatial survey data from 92 households each in UG and TZ this study found that, from wet to dry season, 26% (UG) and 9% (TZ) of households switched from a source with higher risk of contamination to a source with lower risk. By comparison, only 20% (UG) and 0% (TZ) of households switched from a source with lower risk of contamination to a source with higher risk of contamination. This research suggests that one pathway through which water-related disease prevalence may differ across seasons is the use of water sources with higher risk contamination, and that households with access to sources with lower risks of contamination sometimes choose to use more contaminated sources.
Factors Associated with Relapse among Heroin Addicts: Evidence from a Two-Year Community-Based Follow-Up Study in China
Authors: Rong Chao , Jiang Hai-Feng , Zhang Rui-Wen , Zhang Li-Juan , Zhang Jian-Chen , Zhang Jing , Feng Xue-Shan
Published: January 2016

Background: Many countries including China are facing a serious opiate dependence problem. Anti-drug work effectiveness was affected by the high relapse rate all over the world. This study aims to analyze the factors influencing heroin addict relapse, and to provide evidence for generating relapse prevention strategies. Methods: A community-based follow-up study was conducted in China between October 2010 and September 2012. A total of 554 heroin addicts in accordance with the inclusion criteria from 81 streets in 12 districts of Shanghai, China were divided into 4 groups: group 1—daily dosage taken orally of 60 mL of methadone or under combined with psychological counseling and social supports (n = 130); group 2—daily dosage taken orally of over 60 mL of methadone combined with psychological counseling and social supports (n = 50); group 3—JTT (Jitai tablets) combined with psychological counseling and social supports (n = 206); group 4—JTT combined with social supports (n = 168). Results: Log-rank test results showed that the cumulative relapse rate differences among four groups during the two-year follow-up period were not statistically significant (χ2 = 5.889, p = 0.117). Multivariate Cox regression analysis results showed that only three independent variables were still statistically significant, including compliance with participation in psychological counseling (OR = 3.563, p = 0.000), the years of drug use (OR = 1.078, p = 0.001)and intervention model. Conclusions: Using the detoxification medications combined with appropriate psychological counseling and social support measures will help improve the effectiveness of relapse prevention, which is a kind of alternative community detoxification pattern. Appropriate and standard psychological counseling is very important for anti-drug treatment. The longer the drug addiction lasts, the longer the anti-drug treatment takes.
The Metabolic Fate of ortho-Quinones Derived from Catecholamine Metabolites
Authors: Ito Shosuke , Yamanaka Yuta , Ojika Makoto , Wakamatsu Kazumasa
Published: January 2016

ortho-Quinones are produced in vivo through the oxidation of catecholic substrates by enzymes such as tyrosinase or by transition metal ions. Neuromelanin, a dark pigment present in the substantia nigra and locus coeruleus of the brain, is produced from dopamine (DA) and norepinephrine (NE) via an interaction with cysteine, but it also incorporates their alcoholic and acidic metabolites. In this study we examined the metabolic fate of ortho-quinones derived from the catecholamine metabolites, 3,4-dihydroxyphenylethanol (DOPE), 3,4-dihydroxyphenylethylene glycol (DOPEG), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3,4-dihydroxyphenylmandelic acid (DOMA). The oxidation of catecholic substrates by mushroom tyrosinase was followed by UV-visible spectrophotometry. HPLC analysis after reduction with NaBH4 or ascorbic acid enabled measurement of the half-lives of ortho-quinones and the identification of their reaction products. Spectrophotometric examination showed that the ortho-quinones initially formed underwent extensive degradation at pH 6.8. HPLC analysis showed that DOPE-quinone and DOPEG-quinone degraded with half-lives of 15 and 30 min at pH 6.8, respectively, and >100 min at pH 5.3. The major product from DOPE-quinone was DOPEG which was produced through the addition of a water molecule to the quinone methide intermediate. DOPEG-quinone yielded a ketone, 2-oxo-DOPE, through the quinone methide intermediate. DOPAC-quinone and DOMA-quinone degraded immediately with decarboxylation of the ortho-quinone intermediates to form 3,4-dihydroxybenzylalcohol (DHBAlc) and 3,4-dihydroxybenzaldehyde (DHBAld), respectively. DHBAlc-quinone was converted to DHBAld with a half-life of 9 min, while DHBAld-quinone degraded rapidly with a half-life of 3 min. This study confirmed the fact that ortho-quinones from DOPE, DOPEG, DOPAC and DOMA are converted to quinone methide tautomers as common intermediates, through proton rearrangement or decarboxylation. The unstable quinone methides afford stable alcoholic or carbonyl products.
Lingual Epithelial Stem Cells and Organoid Culture of Them
Authors: Hisha Hiroko , Tanaka Toshihiro , Ueno Hiroo
Published: January 2016

As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenance of the lingual epithelium have not been identified. Moreover, a simple and convenient culture method for lingual epithelial stem cells has not yet been established. Recently, we have shown that Bmi1-positive cells, residing at the second or third layer of the epithelial cell layer at the base of the interpapillary pit (IPP), were slow-cycling and could supply keratinized epithelial cells for over one year, indicating that Bmi1-positive cells are long-term lingual epithelial stem cells. In addition, we have developed a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Here, we discuss current progress in the identification of lingual stem cells and future applications of the lingual culture system for studying the regulatory mechanisms of the lingual epithelium and for regenerative medicine.
Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis
Authors: Gening Leonid , Lakhin Andrei , Makarova Irina , Nenasheva Valentina , Andreeva Ludmila , Tarantul Vyacheslav
Published: January 2016

Using a modified radiolabeled primer extension method (we named this modification misGvA—“misincorporation of G versus A”) we have investigated the DNA synthesis and repair at early and late stages of development of loach Misgurnus fossilis. The misincorporation activity of DNA polymerase iota (Pol ι) in wild-type loach could not be detected by this method at any stage of loach development. In transgenic loach overexpressing human Pol ι we have shown that the bypassing of DNA synthesis arrest after incorporation of mismatched nucleotide by Pol ι (the T-stop) was not associated with this enzyme. Non-transgenic loach larvae are virtually lacking the capacity for error correction of DNA duplex containing a mismatched nucleotide. Such repair activity develops only in the adult fish. It appears that the initial stages of development are characterized by more intensive DNA synthesis, while in terminal stages the repair activities become more prominent. The misGvA approach clearly indicates substantial changes in the DNA synthesis intensity, although the role of particular replicative and repair DNA polymerases in this process requires further study.
The Changing Landscape of Molecular Diagnostic Testing: Implications for Academic Medical Centers
Authors: Rehm Heidi , Hynes Elizabeth , Funke Birgit
Published: January 2016

Over the last decade, the field of molecular diagnostics has undergone tremendous transformation, catalyzed by the clinical implementation of next generation sequencing (NGS). As technical capabilities are enhanced and current limitations are addressed, NGS is increasingly capable of detecting most variant types and will therefore continue to consolidate and simplify diagnostic testing. It is likely that genome sequencing will eventually serve as a universal first line test for disorders with a suspected genetic origin. Academic Medical Centers (AMCs), which have been at the forefront of this paradigm shift are now presented with challenges to keep up with increasing technical, bioinformatic and interpretive complexity of NGS-based tests in a highly competitive market. Additional complexity may arise from altered regulatory oversight, also triggered by the unprecedented scope of NGS-based testing, which requires new approaches. However, these challenges are balanced by unique opportunities, particularly at the interface between clinical and research operations, where AMCs can capitalize on access to cutting edge research environments and establish collaborations to facilitate rapid diagnostic innovation. This article reviews present and future challenges and opportunities for AMC associated molecular diagnostic laboratories from the perspective of the Partners HealthCare Laboratory for Molecular Medicine (LMM).
Acknowledgement to Reviewers of JSAN in 2015
Authors:   JSAN Editorial Office
Published: January 2016

The editors of JSAN would like to express their sincere gratitude to the following reviewers for assessing manuscripts in 2015. [...]
Evaluation of Castor Oil Cake Starch and Recovered Glycerol and Development of “Green” Composites Based on Those with Plant Fibers
Authors: Guimarães José , Trindade Cursino Ana , Ketzer Saul Cyro , Sierrakowski Maria , Ramos Luiz , Satyanarayana Kestur
Published: January 2016

Continuous efforts are being made in some countries for the recovery of crude glycerin (RG/CG) and castor oil cake (COC), the two byproducts of biodiesel production. These are expected to help, not only in addressing environmental safety, but also in adding value to those byproducts, which otherwise may go to waste. Finding ways to utilize those byproducts underlines the main objective of this study. This paper presents the evaluation of (i) COC, glycerin and banana and sugarcane fibers for moisture content; (ii) COC for structural and thermal properties; and (iii) CG for its chemical characteristics. The possibility of using COC and CG with the selected fibers as reinforcement in the development of bio-composites is attempted through thermo-molding. Results revealed enhanced mechanical properties for these composites. The obtained results are discussed in terms of the observed morphology.
Hybrid ABC Optimized MARS-Based Modeling of the Milling Tool Wear from Milling Run Experimental Data
Authors: García Nieto Paulino , García-Gonzalo Esperanza , Ordóñez Galán Celestino , Bernardo Sánchez Antonio
Published: January 2016

Milling cutters are important cutting tools used in milling machines to perform milling operations, which are prone to wear and subsequent failure. In this paper, a practical new hybrid model to predict the milling tool wear in a regular cut, as well as entry cut and exit cut, of a milling tool is proposed. The model was based on the optimization tool termed artificial bee colony (ABC) in combination with multivariate adaptive regression splines (MARS) technique. This optimization mechanism involved the parameter setting in the MARS training procedure, which significantly influences the regression accuracy. Therefore, an ABC–MARS-based model was successfully used here to predict the milling tool flank wear (output variable) as a function of the following input variables: the time duration of experiment, depth of cut, feed, type of material, etc. Regression with optimal hyperparameters was performed and a determination coefficient of 0.94 was obtained. The ABC–MARS-based model's goodness of fit to experimental data confirmed the good performance of this model. This new model also allowed us to ascertain the most influential parameters on the milling tool flank wear with a view to proposing milling machine's improvements. Finally, conclusions of this study are exposed.
Emergent Sources of Prebiotics: Seaweeds and Microalgae
Authors: De Jesus Raposo Maria , De Morais Alcina , De Morais Rui
Published: January 2016

In recent years, scientists have become aware that human microbiota, in general, and gut microbiota, in particular, play a major role in human health and diseases, such as obesity and diabetes, among others. A large number of evidence has come to light regarding the beneficial effects, either for the host or the gut microbiota, of some foods and food ingredients or biochemical compounds. Among these, the most promising seem to be polysaccharides (PS) or their derivatives, and they include the dietary fibers. Some of these PS can be found in seaweeds and microalgae, some being soluble fibers, such as alginates, fucoidans, carrageenans and exopolysaccharides, that are not fermented, at least not completely, by colonic microbiota. This review gives an overview of the importance of the dietary fibers, as well as the benefits of prebiotics, to human health. The potential of the PS from marine macro- and microalgae to act as prebiotics is discussed, and the different techniques to obtain oligosaccharides from PS are presented. The mechanisms of the benefits of fiber, in general, and the types and benefits of algal fibers in human health are highlighted. The findings of some recent studies that present the potential effects of prebiotics on animal models of algal biomass and their extracts, as well as oligo- and polysaccharides, are presented. In the future, the possibility of using prebiotics to modulate the microbiome, and, consequently, prevent certain human diseases is foreseen.
Yessotoxin, a Promising Therapeutic Tool
Authors: Alfonso Amparo , Vieytes Mercedes , Botana Luis
Published: January 2016

Yessotoxin (YTX) is a polyether compound produced by dinoflagellates and accumulated in filter feeding shellfish. No records about human intoxications induced by this compound have been published, however it is considered a toxin. Modifications in second messenger levels, protein levels, immune cells, cytoskeleton or activation of different cellular death types have been published as consequence of YTX exposure. This review summarizes the main intracellular pathways modulated by YTX and their pharmacological and therapeutic implications.
Acknowledgement to Reviewers of Medicines in 2015
Authors:   Medicines Editorial Office
Published: January 2016

The editors of Medicines would like to express their sincere gratitude to the following reviewers for assessing manuscripts in 2015. [...]
Antibacterial Activity and Cytotoxicity of Silver(I) Complexes of Pyridine and (Benz)Imidazole Derivatives. X-ray Crystal Structure of [Ag(2,6-di(CH2OH)py)2]NO3
Authors: Kalinowska-Lis Urszula , Felczak Aleksandra , Chęcińska Lilianna , Szabłowska-Gadomska Ilona , Patyna Emila , Małecki Maciej , Lisowska Katarzyna , Ochocki Justyn
Published: January 2016

Selected aspects of the biological activity of a series of six nitrate silver(I) complexes with pyridine and (benz)imidazole derivatives were investigated. The present study evaluated the antibacterial activities of the complexes against three Gram-negative strains: Pseudomonas aeruginosa ATCC 15442, Escherichia coli ATCC 25922 and Proteus hauseri ATCC 13315. The results were compared with those of silver nitrate, a silver sulfadiazine drug and appropriate ligands. The most significant antibacterial properties were exerted by silver(I) complexes containing benzimidazole derivatives. The cytotoxic activity of the complexes was examined against B16 (murine melanoma) and 10T1/2 (murine fibroblasts) cells. All of the tested silver(I) compounds were not toxic to fibroblast cells in concentration inhibited cancer cell (B16) viability by 50%, which ranged between 2.44–28.65 µM. The molecular and crystal structure of silver(I) complex of 2,6-di(hydroxymethyl)pyridine was determined by single-crystal X-ray diffraction analysis. The most important features of the crystal packing and intermolecular non-covalent interactions in the Ag(I) complex were quantified via Hirshfeld surface analysis.
Chemoinformatics: Achievements and Challenges, a Personal View
Authors: Gasteiger Johann
Published: January 2016

Chemoinformatics provides computer methods for learning from chemical data and for modeling tasks a chemist is facing. The field has evolved in the past 50 years and has substantially shaped how chemical research is performed by providing access to chemical information on a scale unattainable by traditional methods. Many physical, chemical and biological data have been predicted from structural data. For the early phases of drug design, methods have been developed that are used in all major pharmaceutical companies. However, all domains of chemistry can benefit from chemoinformatics methods; many areas that are not yet well developed, but could substantially gain from the use of chemoinformatics methods. The quality of data is of crucial importance for successful results. Computer-assisted structure elucidation and computer-assisted synthesis design have been attempted in the early years of chemoinformatics. Because of the importance of these fields to the chemist, new approaches should be made with better hardware and software techniques. Society’s concern about the impact of chemicals on human health and the environment could be met by the development of methods for toxicity prediction and risk assessment. In conjunction with bioinformatics, our understanding of the events in living organisms could be deepened and, thus, novel strategies for curing diseases developed. With so many challenging tasks awaiting solutions, the future is bright for chemoinformatics.
Binding between Saikosaponin C and Human Serum Albumin by Fluorescence Spectroscopy and Molecular Docking
Authors: Chen Yi-Cun , Wang Hong-Mei , Niu Qing-Xia , Ye Dan-Yan , Liang Guo-Wu
Published: January 2016

Saikosaponin C (SSC) is one of the major active constituents of dried Radix bupleuri root (Chaihu in Chinese) that has been widely used in China to treat a variety of conditions, such as liver disease, for many centuries. The binding of SSC to human serum albumin (HSA) was explored by fluorescence, circular dichroism (CD), UV-vis spectrophotometry, and molecular docking to understand both the pharmacology and the basis of the clinical use of SSC/Chaihu. SSC produced a concentration-dependent quenching effect on the intrinsic fluorescence of HSA, accompanied by a blue shift in the fluorescence spectra. The Stern-Volmer equation showed that this quenching was dominated by static quenching. The binding constant of SSC with HSA was 3.72 × 103 and 2.99 × 103 L·mol−1 at 26 °C and 36 °C, respectively, with a single binding site on each SSC and HSA molecule. Site competitive experiments demonstrated that SSC bound to site I (subdomain IIA) and site II (subdomain IIIA) in HSA. Analysis of thermodynamic parameters indicated that hydrogen bonding and van der Waals forces were mostly responsible for SSC-HSA association. The energy transfer efficiency and binding distance between SSC and HSA was calculated to be 0.23 J and 2.61 nm at 26 °C, respectively. Synchronous fluorescence and CD measurements indicated that SSC affected HSA conformation in the SSC-HSA complex. Molecular docking supported the experimental findings in conformational changes, binding sites and binding forces, and revealed binding of SSC at the interface between subdomains IIA-IIB.
Synthesis, Structure and Cytotoxic Activity of Mono- and Dialkoxy Derivatives of 5,8-Quinolinedione
Authors: Kadela Monika , Jastrzębska Maria , Bębenek Ewa , Chrobak Elwira , Latocha Małgorzata , Kusz Joachim , Książek Maria , Boryczka Stanisław
Published: January 2016

A series of 5,8-quinolinedione derivatives containing one or two alkoxy groups was synthesized and characterized by 1H- and 13C-NMR, IR and MS spectra. X-ray diffraction was used to investigate the crystal structures of 6-chloro-7-(2-cyjanoethoxy)-5,8-quinolinedione and 6,7-di(2,2,2-trifloroethoxy)-5,8-quinolinedione. All studied compounds were tested in vitro for their antiproliferative activity against three human cancer cell lines and human normal fibroblasts. Most of the compounds showed higher cytotoxicity than the starting compound, 6,7-dichloro-5,8-quinolinedione, and cisplatin, which was used as a reference agent.
Regulation of Candida albicans Interaction with Macrophages through the Activation of HOG Pathway by Genistein
Authors: Cui Shuna , Hassan Rabeay , Heintz-Buschart Anna , Bilitewski Ursula
Published: January 2016

The severity of infections caused by Candida albicans, the most common opportunistic human fungal pathogen, needs rapid and effective antifungal treatments. One of the effective ways is to control the virulence factors of the pathogen. Therefore, the current study examined the effects of genistein, a natural isoflavone present in soybeans, on C. albicans. The genistein-treated C. albicans cells were then exposed to macrophages. Although no inhibition effect on the growth rates of C. albicans was noted an enhancement of the immune response to macrophages has been observed, indicated by phagocytosis and release of cytokines TNF-α and IL-10. The effect of genistein on the enhanced phagocytosis can be mimicked by the fungicides fludioxonil or iprodione, which inhibit the histidine kinase Cos1p and lead to activation of HOG pathway. The western blot results showed a clear phosphorylation of Hog1p in the wild type strain of C. albicans after incubation with genistein. In addition, effects of genistein on the phosphorylation of Hog1p in the histidine kinase mutants Δcos1 and Δsln1 were also observed. Our results thus indicate a new bio-activity of genistein on C. albicans by activation of the HOG pathway of the human pathogen C. albicans.
Iron-Folic Acid Supplementation During Pregnancy Reduces the Risk of Stunting in Children Less Than 2 Years of Age: A Retrospective Cohort Study from Nepal
Authors: Nisar Yasir , Dibley Michael , Aguayo Victor
Published: January 2016

The aim of the study was to investigate the effect of antenatal iron-folic acid (IFA) supplementation on child stunting in Nepalese children age <2 years. A retrospective cohort study design was used, in which a pooled cohort of 5235 most recent live births 2 years prior to interview from three Nepal Demographic and Health Surveys (2001, 2006 and 2011) was analysed. The primary outcome was stunting in children age <2 years. The main exposure variable was antenatal IFA supplementation. Multivariate Poisson regression analysis was performed. In our sample, 31% and 10% of Nepalese children age <2 years were stunted and severely stunted, respectively. The adjusted relative risk of being stunted was 14% lower in children whose mothers used IFA supplements compared to those whose mothers did not use (aRR = 0.86, 95% CI = 0.77–0.97). Additionally, the adjusted relative risk of being stunted was significantly reduced by 23% when antenatal IFA supplementation was started ≤6 months with ≥90 IFA supplements used during pregnancy (aRR = 0.77, 95% CI = 0.64–0.92). Antenatal IFA supplementation significantly reduced the risk of stunting in Nepalese children age <2 years. The greatest impact on the risk reduction of child stunting was when IFA supplements were started ≤6 months with ≥90 supplements were used.
B Vitamins and the Brain: Mechanisms, Dose and Efficacy—A Review
Authors: Kennedy David
Published: January 2016

The B-vitamins comprise a group of eight water soluble vitamins that perform essential, closely inter-related roles in cellular functioning, acting as co-enzymes in a vast array of catabolic and anabolic enzymatic reactions. Their collective effects are particularly prevalent to numerous aspects of brain function, including energy production, DNA/RNA synthesis/repair, genomic and non-genomic methylation, and the synthesis of numerous neurochemicals and signaling molecules. However, human epidemiological and controlled trial investigations, and the resultant scientific commentary, have focused almost exclusively on the small sub-set of vitamins (B9/B12/B6) that are the most prominent (but not the exclusive) B-vitamins involved in homocysteine metabolism. Scant regard has been paid to the other B vitamins. This review describes the closely inter-related functions of the eight B-vitamins and marshals evidence suggesting that adequate levels of all members of this group of micronutrients are essential for optimal physiological and neurological functioning. Furthermore, evidence from human research clearly shows both that a significant proportion of the populations of developed countries suffer from deficiencies or insufficiencies in one or more of this group of vitamins, and that, in the absence of an optimal diet, administration of the entire B-vitamin group, rather than a small sub-set, at doses greatly in excess of the current governmental recommendations, would be a rational approach for preserving brain health.
Testing of Candidate Icons to Identify Acetaminophen-Containing Medicines
Authors: Shiffman Saul , Cotton Helene , Jessurun Christina , Sembower Mark , Pype Steve , Phillips Jerry
Published: January 2016

Adding icons on labels of acetaminophen-containing medicines could help users identify the active ingredient and avoid concomitant use of multiple medicines containing acetaminophen. We evaluated five icons for communication effectiveness. Adults (n = 300) were randomized to view a prescription container label or over-the-counter labels with either one or two icons. Participants saw two icon candidates, and reported their interpretation; experts judged whether these reflected critical confusions that might cause harm. Participants rated how effectively each icon communicated key messages. Icons based on abbreviations of “acetaminophen” (“Ac”, “Ace”, “Acm”) were rated less confusing and more effective in communicating the active ingredient than icons based on “APAP” or an abstract symbol. Icons did not result in critical confusion when seen on a readable medicine label. Icon implementation on prescription labels was more effective at communicating the warning against concomitant use than implementation on over-the-counter (OTC) labels. Adding an icon to a second location on OTC labels did not consistently enhance this communication, but reduced rated effectiveness of acetaminophen ingredient communication among participants with limited health literacy. The abbreviation-based icons seem most suitable for labeling acetaminophen-containing medications to enable users to identify acetaminophen-containing products.
Hierarchical Object-Based Mapping of Riverscape Units and in-Stream Mesohabitats Using LiDAR and VHR Imagery
Authors: Demarchi Luca , Bizzi Simone , Piégay Hervé
Published: January 2016

In this paper, we present a new, semi-automated methodology for mapping hydromorphological indicators of rivers at a regional scale using multisource remote sensing (RS) data. This novel approach is based on the integration of spectral and topographic information within a multilevel, geographic, object-based image analysis (GEOBIA). Different segmentation levels were generated based on the two sources of Remote Sensing (RS) data, namely very-high spatial resolution, near-infrared imagery (VHR) and high-resolution LiDAR topography. At each level, different input object features were tested with Machine Learning classifiers for mapping riverscape units and in-stream mesohabitats. The GEOBIA approach proved to be a powerful tool for analyzing the river system at different levels of detail and for coupling spectral and topographic datasets, allowing for the delineation of the natural fluvial corridor with its primary riverscape units (e.g., water channel, unvegetated sediment bars, riparian densely-vegetated units, etc.) and in-stream mesohabitats with a high level of accuracy, respectively of K = 0.91 and K = 0.83. This method is flexible and can be adapted to different sources of data, with the potential to be implemented at regional scales in the future. The analyzed dataset, composed of VHR imagery and LiDAR data, is nowadays increasingly available at larger scales, notably through European Member States. At the same time, this methodology provides a tool for monitoring and characterizing the hydromorphological status of river systems continuously along the entire channel network and coherently through time, opening novel and significant perspectives to river science and management, notably for planning and targeting actions.
Study of the Effect of Temporal Sampling Frequency on DSCOVR Observations Using the GEOS-5 Nature Run Results (Part I): Earth’s Radiation Budget
Authors: Holdaway Daniel , Yang Yuekui
Published: January 2016

Satellites always sample the Earth-atmosphere system in a finite temporal resolution. This study investigates the effect of sampling frequency on the satellite-derived Earth radiation budget, with the Deep Space Climate Observatory (DSCOVR) as an example. The output from NASA’s Goddard Earth Observing System Version 5 (GEOS-5) Nature Run is used as the truth. The Nature Run is a high spatial and temporal resolution atmospheric simulation spanning a two-year period. The effect of temporal resolution on potential DSCOVR observations is assessed by sampling the full Nature Run data with 1-h to 24-h frequencies. The uncertainty associated with a given sampling frequency is measured by computing means over daily, monthly, seasonal and annual intervals and determining the spread across different possible starting points. The skill with which a particular sampling frequency captures the structure of the full time series is measured using correlations and normalized errors. Results show that higher sampling frequency gives more information and less uncertainty in the derived radiation budget. A sampling frequency coarser than every 4 h results in significant error. Correlations between true and sampled time series also decrease more rapidly for a sampling frequency less than 4 h.
Hyperspectral Imagery Classification Using Sparse Representations of Convolutional Neural Network Features
Authors: Liang Heming , Li Qi
Published: January 2016

In recent years, deep learning has been widely studied for remote sensing image analysis. In this paper, we propose a method for remotely-sensed image classification by using sparse representation of deep learning features. Specifically, we use convolutional neural networks (CNN) to extract deep features from high levels of the image data. Deep features provide high level spatial information created by hierarchical structures. Although the deep features may have high dimensionality, they lie in class-dependent sub-spaces or sub-manifolds. We investigate the characteristics of deep features by using a sparse representation classification framework. The experimental results reveal that the proposed method exploits the inherent low-dimensional structure of the deep features to provide better classification results as compared to the results obtained by widely-used feature exploration algorithms, such as the extended morphological attribute profiles (EMAPs) and sparse coding (SC).
Performance Evaluation of the New Compound-Carrier-Modulated Signal for Future Navigation Signals
Authors: Luo Ruidan , Xu Ying , Yuan Hong
Published: January 2016

Navigation Signal based on Compound Carrier (NSCC), is proposed as the potential future global navigation satellite system (GNSS) signal modulation scheme. NSCC, a kind of multi-carrier (MC) signal, is generated by superposition and multi-parameter adjustment of sub-carriers. Therefore, a judious choice of parameter configation is needed. The main objective of this paper is to investigate the performance of the NSCC which is influenced by these parameters and to demonstrate its structure characteristics and superiority, employing a comprehensive evaluation system. The results show that the proposed NSCC signal processes full spectral efficiency and limited out of band (OOB) emissions, satisfying the demands of crowed frequency resources. It also presents better performance in terms of spectral separation coefficients (SSCs), tracking accuracy, multipath mitigation capability and anti-jamming reduction compared with the legacy navigation signals. NSCC modulation represents a serious candidate for navigation satellite augmentation systems, especially for signals applied in challenging environments.
An Online Continuous Human Action Recognition Algorithm Based on the Kinect Sensor
Authors: Zhu Guangming , Zhang Liang , Shen Peiyi , Song Juan
Published: January 2016

Continuous human action recognition (CHAR) is more practical in human-robot interactions. In this paper, an online CHAR algorithm is proposed based on skeletal data extracted from RGB-D images captured by Kinect sensors. Each human action is modeled by a sequence of key poses and atomic motions in a particular order. In order to extract key poses and atomic motions, feature sequences are divided into pose feature segments and motion feature segments, by use of the online segmentation method based on potential differences of features. Likelihood probabilities that each feature segment can be labeled as the extracted key poses or atomic motions, are computed in the online model matching process. An online classification method with variable-length maximal entropy Markov model (MEMM) is performed based on the likelihood probabilities, for recognizing continuous human actions. The variable-length MEMM method ensures the effectiveness and efficiency of the proposed CHAR method. Compared with the published CHAR methods, the proposed algorithm does not need to detect the start and end points of each human action in advance. The experimental results on public datasets show that the proposed algorithm is effective and highly-efficient for recognizing continuous human actions.
Improving IMES Localization Accuracy by Integrating Dead Reckoning Information
Authors: Fujii Kenjiro , Arie Hiroaki , Wang Wei , Kaneko Yuto , Sakamoto Yoshihiro , Schmitz Alexander , Sugano Shigeki
Published: January 2016

Indoor positioning remains an open problem, because it is difficult to achieve satisfactory accuracy within an indoor environment using current radio-based localization technology. In this study, we investigate the use of Indoor Messaging System (IMES) radio for high-accuracy indoor positioning. A hybrid positioning method combining IMES radio strength information and pedestrian dead reckoning information is proposed in order to improve IMES localization accuracy. For understanding the carrier noise ratio versus distance relation for IMES radio, the signal propagation of IMES radio is modeled and identified. Then, trilateration and extended Kalman filtering methods using the radio propagation model are developed for position estimation. These methods are evaluated through robot localization and pedestrian localization experiments. The experimental results show that the proposed hybrid positioning method achieved average estimation errors of 217 and 1846 mm in robot localization and pedestrian localization, respectively. In addition, in order to examine the reason for the positioning accuracy of pedestrian localization being much lower than that of robot localization, the influence of the human body on the radio propagation is experimentally evaluated. The result suggests that the influence of the human body can be modeled.
DeepSurveyCam—A Deep Ocean Optical Mapping System
Authors: Kwasnitschka Tom , Köser Kevin , Sticklus Jan , Rothenbeck Marcel , Weiß Tim , Wenzlaff Emanuel , Schoening Timm , Triebe Lars , Steinführer Anja , Devey Colin , Greinert Jens
Published: January 2016

Underwater photogrammetry and in particular systematic visual surveys of the deep sea are by far less developed than similar techniques on land or in space. The main challenges are the rough conditions with extremely high pressure, the accessibility of target areas (container and ship deployment of robust sensors, then diving for hours to the ocean floor), and the limitations of localization technologies (no GPS). The absence of natural light complicates energy budget considerations for deep diving flash-equipped drones. Refraction effects influence geometric image formation considerations with respect to field of view and focus, while attenuation and scattering degrade the radiometric image quality and limit the effective visibility. As an improvement on the stated issues, we present an AUV-based optical system intended for autonomous visual mapping of large areas of the seafloor (square kilometers) in up to 6000 m water depth. We compare it to existing systems and discuss tradeoffs such as resolution vs. mapped area and show results from a recent deployment with 90,000 mapped square meters of deep ocean floor.