bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimation of Energy Profile and Possible Energy Savings of Unclassified Buildings
Authors: Geikins Aleksandrs , Borodinecs Anatolijs , Jacnevs Vladislavs
Published: July 2022

In the European Union, only 1% of the building stock is renovated every year. According to the EU strategy, around 75% of the existing building stock needs to be renovated by 2050. Energy efficiency programs mainly support residential and public building stocks; this article considers military dormitories as a type of unclassified building. It is very important to improve energy efficiency to reduce energy consumption and improve the microclimate in these buildings, since the staff is there 24/7. This paper analyzes the energy consumption and measures the indoor air quality in 13 nonrenovated military dormitories. The personnel in unclassified buildings have limited options for remote work in the case of COVID-19 outbreak. Thus, the retrofitting and maintenance of such buildings must be planned carefully. There is a significant lack of IAQ measurements in unclassified buildings. This study presents a wide analysis of energy consumption, indoor air parameters, and occupant satisfaction. On the basis of real data, four retrofitting scenarios were evaluated in IDA ICE dynamic simulation software. The simulation results showed that, in the case of a deep renovation scenario, the theoretical energy savings could be 77.6–79.3% of the used energy. This paper discusses the solar energy potential of onsite energy production for increasing the efficiency and energy supply resilience of unclassified buildings. The results of this study can be applied to other countries with climate conditions similar to Latvia.
Good and Bad Stroma in Pancreatic Cancer: Relevance of Functional States of Cancer-Associated Fibroblasts
Authors: Ando Ryota , Sakai Akihiro , Iida Tadashi , Kataoka Kunio , Mizutani Yasuyuki , Enomoto Atsushi
Published: July 2022

A well-known feature of human pancreatic ductal adenocarcinoma (PDAC) is the extensive proliferation of cancer-associated fibroblasts (CAFs) and highly fibrotic stroma. Recent evidence, based mainly on single-cell analyses, has identified various subsets of CAFs in PDAC mouse models. However, we do not know how these CAF subsets are involved in the progression and drug resistance of human PDAC. Additionally, it remains unclear whether these diverse CAFs have distinct origins and are indicators of genuinely distinct CAF lineages or reflect different states of the same CAFs depending on the tumor microenvironment. Interestingly, recent preclinical studies have started to characterize the nature of cancer-restraining CAFs and have identified their markers Meflin and collagen type I alpha 1. These studies have led to the development of strategies to induce changes in CAF phenotypes using chemical reagents or recombinant viruses, and some of them have been tested in clinical studies. These strategies have the unique potential to convert the so-called bad stroma to good stroma and may also have therapeutic implications for non-cancer diseases such as fibrotic diseases. Together with recently developed sophisticated strategies that specifically target distinct CAF subsets via adoptive cell transfer therapy, vaccination, and antibody–drug conjugates, any future findings arising from these clinical efforts may expand our understanding of the significance of CAF diversity in human PDAC.
Temporal Changes in Sparing and Enhancing Dose Protraction Effects of Ionizing Irradiation for Aortic Damage in Wild-Type Mice
Authors: Hamada Nobuyuki , Kawano Ki-ichiro , Nomura Takaharu , Furukawa Kyoji , Yusoff Farina Mohamad , Maruhashi Tatsuya , Maeda Makoto , Nakashima Ayumu , Higashi Yukihito
Published: July 2022

In medical and occupational settings, ionizing irradiation of the circulatory system occurs at various dose rates. We previously found sparing and enhancing dose protraction effects for aortic changes in wild-type mice at 6 months after starting irradiation with 5 Gy of photons. Here, we further analyzed changes at 12 months after stating irradiation. Irrespective of irradiation regimens, irradiation little affected left ventricular function, heart weight, and kidney weight. Irradiation caused structural disorganizations and intima-media thickening in the aorta, along with concurrent elevations of markers for proinflammation, macrophage, profibrosis, and fibrosis, and reductions in markers for vascular functionality and cell adhesion in the aortic endothelium. These changes were qualitatively similar but quantitatively less at 12 months than at 6 months. The magnitude of such changes at 12 months was not smaller in 25 fractions (Frs) but was smaller in 100 Frs and chronic exposure than acute exposure. The magnitude at 6 and 12 months was greater in 25 Frs, smaller in 100 Frs, and much smaller in chronic exposure than acute exposure. These findings suggest that dose protraction changes aortic damage, in a fashion that depends on post-irradiation time and is not a simple function of dose rate.
Molecular Targets in Lung Cancer: Study of the Evolution of Biomarkers Associated with Treatment with Tyrosine Kinase Inhibitors—Has NF1 Tumor Suppressor a Key Role in Acquired Resistance?
Authors: Alen Begoña O. , Estévez-Pérez Lara S. , Hermida-Romero María Teresa , Reguera-Arias Ana , García-Campelo Rosario , de la Torre-Bravos Mercedes , Concha Ángel
Published: July 2022

The application to clinical practice of liquid biopsy in patients with lung cancer has led to an advance in the diagnosis and monitoring of the disease. Detection of alterations in EGFR genes related to TKI treatment in EGFR-mutated non-small cell lung cancer patients is a routine method in pathology laboratories. The primary objective of this work was to analyze the presence of EGFR mutations in cfDNA of 86 patients with lung cancer undergoing oncological treatment related to response to treatment with TKIs. Secondarily, we evaluated the dynamics of EGFR mutations, the presence of the T790M alteration and its relationship with drug resistance and analyzed by NGS molecular alterations in cfDNA of patients with discordant progression. Our results demonstrate that understanding the mutational status of patients treated with TKIs over time is essential to monitor disease progression. In this context, liquid biopsy is a fundamental key. In addition, it is not only necessary to detect EGFR mutations, but also other concomitant mutations that would be influencing the development of the disease. In this sense, we have discovered that mutations in the NF1 tumor suppressor gene could be exerting an as yet unknown function in lung cancer.
Advances in the In Vivo Quantitative and Qualitative Imaging Characterization of Gliomas
Authors: Alongi Pierpaolo , Vetrano Ignazio Gaspare
Published: July 2022

Gliomas are the most common and aggressive intra-axial primary tumours of the central nervous system (CNS), arising from glial cells [...]
The Urogenital System’s Role in Diseases: A Synopsis
Authors: Zerdan Maroun Bou , Moukarzel Rita , Naji Nour Sabiha , Bilen Yara , Nagarajan Arun
Published: July 2022

The human microbiota contains ten times more microbial cells than human cells contained by the human body, constituting a larger genetic material than the human genome itself. Emerging studies have shown that these microorganisms represent a critical determinant in human health and disease, and the use of probiotic products as potential therapeutic interventions to modulate homeostasis and treat disease is being explored. The gut is a niche for the largest proportion of the human microbiota with myriad studies suggesting a strong link between the gut microbiota composition and disease development throughout the body. More specifically, there is mounting evidence on the relevance of gut microbiota dysbiosis in the development of urinary tract disease including urinary tract infections (UTIs), chronic kidney disease, and kidney stones. Fewer emerging reports, however, are suggesting that the urinary tract, which has long been considered ‘sterile’, also houses its unique microbiota that might have an important role in urologic health and disease. The implications of this new paradigm could potentially change the therapeutic perspective in urological disease.
Studying Epigenetics of Cardiovascular Diseases on Chip Guide
Authors: Alghamdi Bandar , Aljohani Intisar , Alotaibi Bandar , Ahmed Muhammad , Almazmomi Kholod , Aloufi Salman , Alshamrani Jowhra
Published: July 2022

Epigenetics is defined as the study of inheritable changes in the gene expressions and phenotypes that occurs without altering the normal DNA sequence. These changes are mainly due to an alteration in chromatin or its packaging, which changes the DNA accessibility. DNA methylation, histone modification, and noncoding or microRNAs can best explain the mechanism of epigenetics. There are various DNA methylated enzymes, histone-modifying enzymes, and microRNAs involved in the cause of various CVDs (cardiovascular diseases) such as cardiac hypertrophy, heart failure, and hypertension. Moreover, various CVD risk factors such as diabetes mellitus, hypoxia, aging, dyslipidemia, and their epigenetics are also discussed together with CVDs such as CHD (coronary heart disease) and PAH (pulmonary arterial hypertension). Furthermore, different techniques involved in epigenetic chromatin mapping are explained. Among these techniques, the ChIP-on-chip guide is explained with regard to its role in cardiac hypertrophy, a final form of heart failure. This review focuses on different epigenetic factors that are involved in causing cardiovascular diseases.
A Novel Model Parameter for the Assessment of the Dimerization of n-Butenes over Ni-Containing Aluminosilicate Catalysts
Authors: Alscher Felix , Borovinskaya Ekaterina , Peitz Stephan , Reschetilowski Wladimir
Published: July 2022

Previous investigations on the dimerization of n-butenes over Ni-containing Al-MCM-41/ZSM-5 mixed-phase catalysts have shown a correlation between the conversion and the Ni/(Al + Ni) ratio as well as between the conversion and the metal–support interaction, which is represented by the reduction degree. In the present work, both approaches are combined to a novel model parameter, which allows to determine the proportion of active Ni species on the Al-Ni site pairs and thus provides crucial insights into formation of catalytically active nickel sites as well as their effects on the selective formation of low-branched dimers.
Three-Step Purification and Characterization of Organic Solvent-Tolerant and Alkali-Thermo-Tolerant Xylanase from Bacillus paramycoides T4 [MN370035]
Authors: Tiwari Soni , Singh Ranjan , Yadav Janardan , Gaur Rajeeva , Singh Anurag , Yadav Jay Shankar , Pandey Prabhash Kumar , Yadav Santosh Kumar , Prajapati Jaya , Helena Pukhrambam , Dewangan Jayant , Jamal Farrukh
Published: July 2022

In the present study, an extracellular alkali-thermo-tolerant xylanase from Bacillus paramycoides was produced in the presence of an organic solvent. The enzyme was purified by ammonium sulphate precipitation, gel filtration, and ion exchange chromatography, with an overall recovery of 25.9%. The purified enzyme hada 70 kDa molecular weight (MW) confirmed by SDS-PAGE gel analysis. The maximum enzyme activity was reported at 55 °C and pH 7.0. Xylanase activity and stability were improved in the presence of 30% (v/v) n-dodecane, iso-octane, n-decane, and cyclohexane (7 days). The enzyme activity was improved by Co2+, EDTA, and Triton-X-100 while vigorously repressed by Hg2+ and Cu2+. The purified enzyme showed 1.473 mg/mL Km and 654.017 µg/mL/min Vmax values. The distinctive assets of the isolate verified the potential application in the field of biomass conversion into fuel and other industrial processes. Organic solvent-tolerant xylanases can be used for concurrent saccharification and bioethanol production, the amplification of intoxicating beverages, and the fermenting industry.
Covalent Modification of Iron Phthalocyanine into Skeleton of Graphitic Carbon Nitride and Its Visible-Light-Driven Photocatalytic Reduction of Nitroaromatic Compounds
Authors: Qian Jiajia , Liu Ying , Zheng Weiran , Zhou Baocheng , Dong Xiaoping
Published: July 2022

It is of great urgency to eliminate nitroaromatic compounds (NACs) in wastewater due to their high residue and toxicity. Photocatalysis reduction is considered to be an efficient technology for converting NACs to their corresponding aromatic amines. In this work, a visible-light-driven hybrid photocatalyst was synthesized by covalently doping Fe phthalocyanine (FePc) into graphitic carbon nitride skeleton. Compared to the pristine gCN, the optimized gCN-FePc-1 photocatalyst showed enhanced absorption in visible light region, which promoted photogenerated charge transfer and separation. Using p-nitrophenol (p-NP) as the model pollutant, the CN-FePc-1 effectively reduced it to p-aminophenol (p-AP), with the photocatalytic reaction rate being 18 and 3 times higher, respectively, than those of the pristine gCN and the mechanically mixed photocatalyst of gCN/FePc. Moreover, excellent photocatalytic universality for other NACs, high stability, and good reusability also were confirmed. Based on the band structure of the gCN-FePc-1 photocatalyst, a plausible mechanism was proposed to illustrate the photocatalytic reduction process of p-NP to p-AP. This study demonstrates that the covalent modification of FePc into gCN skeleton is an effective strategy to modulate the electronic structure, and the hybrid gCN-FePc is a potential visible-light-driven photocatalyst that potentially can be used for eliminating NAC contamination in wastewater.
Phyco-Synthesized Zinc Oxide Nanoparticles Using Marine Macroalgae, Ulva fasciata Delile, Characterization, Antibacterial Activity, Photocatalysis, and Tanning Wastewater Treatment
Authors: Fouda Amr , Eid Ahmed M. , Abdelkareem Ayman , Said Hanan A. , El-Belely Ehab F. , Alkhalifah Dalal Hussien M. , Alshallash Khalid S. , Hassan Saad El-Din
Published: July 2022

The aqueous extract of marine green macroalgae, Ulva fasciata Delile, was harnessed for the synthesis of zinc oxide nanoparticles (ZnO-NPs). The conversion to ZnO-NPs was characterized by color change, UV–vis spectroscopy, FT-IR, TEM, SEM-EDX, and XRD. Data showed the formation of spherical and crystalline ZnO-NPs with a size range of 3–33 nm. SEM-EDX revealed the presence of Zn and O in weight percentages of 45.3 and 31.62%, respectively. The phyco-synthesized ZnO-NPs exhibited an effective antibacterial activity against the pathogenic Gram-positive and Gram-negative bacteria. The bacterial clear zones ranged from 21.7 ± 0.6 to 14.7 ± 0.6 mm with MIC values of 50–6.25 µg mL−1. The catalytic activity of our product was investigated in dark and visible light conditions, using the methylene blue (MB) dye. The maximum dye removal (84.9 ± 1.2%) was achieved after 140 min in the presence of 1.0 mg mL−1 of our nanocatalyst under the visible light at a pH of 7 and a temperature of 35 °C. This percentage was decreased to 53.4 ± 0.7% under the dark conditions. This nanocatalyst showed a high reusability with a decreasing percentage of ~5.2% after six successive cycles. Under the optimum conditions, ZnO-NPs showed a high efficacy in decolorizing the tanning wastewater with a percentage of 96.1 ± 1.7%. Moreover, the parameters of the COD, BOD, TSS, and conductivity were decreased with percentages of 88.8, 88.5, 96.9, and 91.5%, respectively. Moreover, nano-ZnO had a high efficacy in decreasing the content of the tanning wastewater Cr (VI) from 864.3 ± 5.8 to 57.3 ± 4.1 mg L−1 with a removal percentage of 93.4%.
Yeast Kinesin-5 Motor Protein CIN8 Promotes Accurate Chromosome Segregation
Authors: Sherwin Delaney , Huetteman Abigail , Wang Yanchang
Published: July 2022

Accurate chromosome segregation depends on bipolar chromosome–microtubule attachment and tension generation on chromosomes. Incorrect chromosome attachment results in chromosome missegregation, which contributes to genome instability. The kinetochore is a protein complex that localizes at the centromere region of a chromosome and mediates chromosome–microtubule interaction. Incorrect chromosome attachment leads to checkpoint activation to prevent anaphase onset. Kinetochore detachment activates the spindle assembly checkpoint (SAC), while tensionless kinetochore attachment relies on both the SAC and tension checkpoint. In budding yeast Saccharomyces cerevisiae, kinesin-5 motor proteins Cin8 and Kip1 are needed to separate spindle pole bodies for spindle assembly, and deletion of CIN8 causes lethality in the absence of SAC. To study the function of Cin8 and Kip1 in chromosome segregation, we constructed an auxin-inducible degron (AID) mutant, cin8-AID. With this conditional mutant, we first confirmed that cin8-AID kip1∆ double mutants were lethal when Cin8 is depleted in the presence of auxin. These cells arrested in metaphase with unseparated spindle pole bodies and kinetochores. We further showed that the absence of either the SAC or tension checkpoint was sufficient to abolish the cell-cycle delay in cin8-AID mutants, causing chromosome missegregation and viability loss. The tension checkpoint-dependent phenotype in cells with depleted Cin8 suggests the presence of tensionless chromosome attachment. We speculate that the failed spindle pole body separation in cin8 mutants could increase the chance of tensionless syntelic chromosome attachments, which depends on functional tension checkpoint for survival.
Pancreatic Transdifferentiation Using β-Cell Transcription Factors for Type 1 Diabetes Treatment
Authors: Mahoney Alexandra L. G. , Nassif Najah T. , O’Brien Bronwyn A. , Simpson Ann M.
Published: July 2022

Type 1 diabetes is a chronic illness in which the native beta (β)-cell population responsible for insulin release has been the subject of autoimmune destruction. This condition requires patients to frequently measure their blood glucose concentration and administer multiple daily exogenous insulin injections accordingly. Current treatments fail to effectively treat the disease without significant side effects, and this has led to the exploration of different approaches for its treatment. Gene therapy and the use of viral vectors has been explored extensively and has been successful in treating a range of diseases. The use of viral vectors to deliver β-cell transcription factors has been researched in the context of type 1 diabetes to induce the pancreatic transdifferentiation of cells to replace the β-cell population destroyed in patients. Studies have used various combinations of pancreatic and β-cell transcription factors in order to induce pancreatic transdifferentiation and have achieved varying levels of success. This review will outline why pancreatic transcription factors have been utilised and how their application can allow the development of insulin-producing cells from non β-cells and potentially act as a cure for type 1 diabetes.
Effect of Upregulation of Transcription Factor TFDP1 Binding Promoter Activity Due to RBP4 g.36491960G>C Mutation on the Proliferation of Goat Granulosa Cells
Authors: Liu Yufang , Guo Siwu , He Xiaoyun , Jiang Yanting , Hong Qionghua , Lan Rong , Chu Mingxing
Published: July 2022

Retinol-binding protein 4 (RBP4), a member of the lipocalin family, is a specific carrier of retinol (vitamin A) in the blood. Numerous studies have shown that RBP4 plays an important role in mammalian embryonic development and that mutations in RBP4 can be used for the marker-assisted selection of animal reproductive traits. However, there are few studies on the regulation of reproduction and high-prolificacy traits by RBP4 in goats. In this study, the 5′ flanking sequence of RBP4 was amplified, and a G>C polymorphism in the promoter region -211 bp (g.36491960) was detected. An association analysis revealed that the respective first, second and third kidding number and mean kidding number of nanny goats with CC and GC genotypes (2.167 ± 0.085, 2.341 ± 0.104, 2.529 ± 0.107 and 2.189 ± 0.070 for CC and 2.052 ± 0.047, 2.206 ± 0.057, 2.341 ± 0.056 and 2.160 ± 0.039 for GC) were significantly higher (p < 0.05) than those with the GG genotype (1.893 ± 0.051, 2.027 ± 0.064, 2.107 ± 0.061 and 1.74 ± 0.05). The luciferase assay showed that luciferase activity was increased in C allele individuals compared with that in G allele individuals. A competitive electrophoretic mobility shift assay (EMSA) showed that individuals with the CC genotype had a stronger promoter region binding capacity than those with the GG genotype. In addition, transcription factor prediction software showed that the RBP4 g.36491960G>C mutation added a novel binding site for transcription factor DP-1 (TFDP1). RT–qPCR results showed that the expression of TFDP1 was significantly higher in the high-prolificacy group than in the low-prolificacy group, and the expression of RBP4 was higher in both the CC and GC genotypes than that in the GG genotype. TFDP1 overexpression significantly increased the expression of RBP4 mRNA (p < 0.05) and the expression of the cell proliferation factors cyclin-D1, cyclin-D2 and CDK4 (p < 0.05). The opposite trend was observed after interference with TFDP1. Both the EdU and CCK-8 results showed that TFDP1 expression could regulate the proliferation of goat ovarian granulosa cells. In summary, our results showed that RBP4 g.36491960G>C was significantly associated with fecundity traits in goats. The g.36491960G>C mutation enhanced the transcriptional activity of RBP4 and increased the expression of RBP4, thus improving the fertility of Yunshang black goats.
The Involvement of microRNAs in Plant Lignan Biosynthesis—Current View
Authors: Ražná Katarína , Harenčár Ľubomír , Kučka Matúš
Published: July 2022

Lignans, as secondary metabolites synthesized within a phenylpropanoid pathway, play various roles in plants, including their involvement in growth and plant defense processes. The health and nutritional benefits of lignans are unquestionable, and many studies have been devoted to these attributes. Although the regulatory role of miRNAs in the biosynthesis of secondary metabolites has been widely reported, there is no systematic review available on the miRNA-based regulatory mechanism of lignans biosynthesis. However, the genetic background of lignan biosynthesis in plants is well characterized. We attempted to put together a regulatory mosaic based on current knowledge describing miRNA-mediated regulation of genes, enzymes, or transcription factors involved in this biosynthesis process. At the same time, we would like to underline the fact that further research is necessary to improve our understanding of the miRNAs regulating plant lignan biosynthesis by exploitation of current approaches for functional identification of miRNAs.
Knockdown of Lamin B1 and the Corresponding Lamin B Receptor Leads to Changes in Heterochromatin State and Senescence Induction in Malignant Melanoma
Authors: Lämmerhirt Lisa , Kappelmann-Fenzl Melanie , Fischer Stefan , Pommer Michaela , Zimmermann Tom , Kluge Viola , Matthies Alexander , Kuphal Silke , Bosserhoff Anja Katrin
Published: July 2022

Modifications in nuclear structures of cells are implicated in several diseases including cancer. They result in changes in nuclear activity, structural dynamics and cell signalling. However, the role of the nuclear lamina and related proteins in malignant melanoma is still unknown. Its molecular characterisation might lead to a deeper understanding and the development of new therapy approaches. In this study, we analysed the functional effects of dysregulated nuclear lamin B1 (LMNB1) and its nuclear receptor (LBR). According to their cellular localisation and function, we revealed that these genes are crucially involved in nuclear processes like chromatin organisation. RNA sequencing and differential gene expression analysis after knockdown of LMNB1 and LBR revealed their implication in important cellular processes driving ER stress leading to senescence and changes in chromatin state, which were also experimentally validated. We determined that melanoma cells need both molecules independently to prevent senescence. Hence, downregulation of both molecules in a BRAFV600E melanocytic senescence model as well as in etoposide-treated melanoma cells indicates both as potential senescence markers in melanoma. Our findings suggest that LMNB1 and LBR influence senescence and affect nuclear processes like chromatin condensation and thus are functionally relevant for melanoma progression.
Adsorption of Lead from Aqueous Solution by Biochar: A Review
Authors: Wang Chuanbin , Wang Xutong , Li Ning , Tao Junyu , Yan Beibei , Cui Xiaoqiang , Chen Guanyi
Published: July 2022

Industrialization has resulted in the discharge of a certain amount of lead (Pb) from industrial sources causing damage risk to water quality and human health. Adsorption is an effective technique to remove Pb, and biochar has been widely studied owing to its advantages of low cost and high adsorption capacity. This review summarizes the influence of raw materials and modification methods on the adsorption capability of biochar. The adsorption isotherms and kinetics of biochar were summarized, and the main Pb removal mechanisms were studied systematically. In addition, the challenges and future perspectives were discussed comprehensively. It is expected that the review could provide insightful fundamentals for the experimental research and practical applications of biochar.
Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis
Authors: Ingiosi Ashley M. , Frank Marcos G.
Published: July 2022

Astrocytes influence sleep expression and regulation, but the cellular signaling pathways involved in these processes are poorly defined. We proposed that astrocytes detect and integrate a neuronal signal that accumulates during wakefulness, thereby leading to increased sleep drive. Noradrenaline (NA) satisfies several criteria for a waking signal integrated by astrocytes. We therefore investigated the role of NA signaling in astrocytes in mammalian sleep. We conditionally knocked out (cKO) β2-adrenergic receptors (β2-AR) selectively in astrocytes in mice and recorded electroencephalographic and electromyographic activity under baseline conditions and in response to sleep deprivation (SDep). cKO of astroglial β2-ARs increased active phase siesta duration under baseline conditions and reduced homeostatic compensatory changes in sleep consolidation and non-rapid eye movement slow-wave activity (SWA) after SDep. Overall, astroglial NA β2-ARs influence mammalian sleep homeostasis in a manner consistent with our proposed model of neuronal–astroglial interactions.
Advanced Biomaterials and Coatings
Authors: Drevet Richard , Benhayoune Hicham
Published: July 2022

Everywhere on Earth, people are living longer and longer [...]
Advanced Research on Energy Storage Materials and Devices
Authors: Zheng Xinyu , Luo Jing , Liu Zheyuan , Wang Qian , Zhang Weidong , Yang Chengkai , Yu Yan
Published: July 2022

With the continuous consumption of global fossil energy and the prevalence of serious environmental problems, renewable and clean energy has attracted increasingly more attention [...]
Structural, Morphological and Optical Properties of Nanostructured ZrO2 Films Obtained by an Electrochemical Process at Different Deposition Temperatures
Authors: Lovchinov Konstantin , Gergova Rositsa , Alexieva Gergana
Published: July 2022

This article focuses on the impact of the deposition temperature (in the range from 60 to 80 °C) in ZrO2 films obtained by the electrochemical deposition process on SnO2-covered glass substrates. The solution in which the deposition takes place is aqueous, containing ZrOCl2 with a concentration of 3 × 10−5 M and KCl with a concentration of 0.1 M. By implementing X-ray diffraction (XRD), optical profilometry, scanning electron microscopy (SEM), and UV-VIS-NIR spectroscopy, the temperature dependence of ZrO2 films properties was revealed. The X-ray Diffraction XRD spectra showed six different diffraction maxima ((−111)M, (101)T, (111)M, (112)M, (202)M, and (103)M) associated with the electrochemical ZrO2 layers, and the polycrystalline structure of the films was confirmed at all deposition temperatures. The determination of the average roughness did not indicate significant temperature dependence in the deposited layers. SEM micrographs showed that the layers were composed of grains, most of them of a regular shape, although their size increased slightly with an increased deposition temperature. The coarsest-grained structure was observed for the layers deposited at 80 °C. It was demonstrated that the deposition temperature weakly impacts the reflectance and transmittance spectra of the ZrO2 layers. Such layers with low values of specular and high values of diffuse transition, and reflection in the spectral range from 380 to 800 nm, can be applied to various optoelectronic devices such as thin-film solar cells.
Preparation and Performance Evaluation of X-ray-Shielding Barium Sulfate Film for Medical Diagnosis Using PET Recycling and Multi-Carrier Principles
Authors: Kim Seon-Chil
Published: July 2022

The use of disposable containers and packaging materials has increased due to the recent COVID-19 pandemic. Thus, the generation of plastic waste is also increasing, and research on recycling such waste is being actively conducted. In this study, an X-ray-shielding film for medical diagnosis was manufactured by mixing a radiation-shielding material and a plastic waste-based polymer material and its effectiveness was evaluated. The film, which is intended as a fabric for a shielding garment, consists of barium sulfate (BaSO4) shielding nanoparticles embedded in a matrix of polyethylene terephthalate (PET), a commonly available waste plastic material. A particle-dispersing technology, which can improve the ratio between the shielding and matrix materials while maintaining the tensile strength of the film, was studied. Therefore, to increase the content of the barium sulfate (BaSO4) nanoparticles used as the shielding material, this multi-carrier method—under which the particles are dispersed in units of time—was developed to improve the shielding performance. Compared with the effectiveness of lead (Pb) shielding film, the 3 mm barium sulfate film developed in this study satisfies the lead equivalent of 0.150 mmPb when stacked in two layers. Therefore, a shielding film was successfully manufactured by using plastic waste as a polymer resin and barium sulfate, an eco-friendly radiation-shielding material, instead of lead.
Proposal of a Model for the Analysis of the State of the Use of ICT in Education Applied to Technological Institutes of Higher Education
Authors: Villegas-Ch. William , Jácome-Vásconez Santiago , García-Ortiz Joselin , Calvache-Sánchez Javier , Sánchez-Viteri Santiago
Published: July 2022

The inclusion of information and communication technologies in education has become a priority for all educational models, particularly for higher education institutes that have observed the need to integrate these technologies in the classroom. However, to guarantee educational quality and learning, establishing a process that allows the identification of the response of the students towards its use is necessary. For this purpose, there are several works that address the issue and have determined the functionality of these technologies, but each environment is different, and this is recognized by the higher education institutes of Ecuador that have limited economic, technological, and academic resources. This work seeks to create a method that allows the needs and doubts of students about the use of educational technologies in the classroom to be established without affecting their academic performance. To perform this, a process has been designed that identifies learning needs through the validation of data obtained from surveys and a comparison of two groups of students, in which one group makes use of technologies in the classroom and the other group uses a model of traditional education. By obtaining the results of the analysis, the method determines the impact of technology on learning.
Effects of Methane Pre-Reforming Percentage and Flow Arrangement on the Distribution of Temperature and Thermal Stress in Solid Oxide Fuel Cell
Authors: Cai Weiqiang , Zheng Qingrong , Yu Wanneng , Yin Zibin , Yuan Jinliang , Zhang Zhonggang , Pei Yuyao
Published: July 2022

To obtain detailed information on the temperature field and thermal stress field inside the solid oxide fuel cell (SOFC) fueled with partially pre-reformed methane. A three-dimensional geometric and mathematical model of the SOFC is implemented by using the finite element method in the commercial software COMSOL Multiphysics®. The coupling characteristics were analyzed for electrode chemical reaction, multi-component mass transfer, and heat transfer process under typical operating conditions, which was further applied for predicting and analyzing the thermal stress distribution. After model validation, parametric simulations are conducted to investigate how the methane pre-reforming percentage and flow arrangement affect the temperature and the thermal stress of SOFC. The simulated results show that reducing the methane pre-reforming percentages can decrease the temperature maximum and the variation range of the first principal stress, but will increase the possibility of carbon deposition. The maximum temperature of the counter-flow is about 20 K lower than that of the co-flow, and the first principal stress variation range of the counter-flow is 8.6 Mpa lower than that of the co-flow. The methane pre-reforming percentages have a significant effect on the heat transfer and the thermal stress, and the counter-flow can improve the temperature uniformity and reduce the thermal stress variation range.
Transformation of 2D RVE Local Stress and Strain Distributions to 3D Observations in Full Phase Crystal Plasticity Simulations of Dual-Phase Steels
Authors: Tseng Shaochen , Qayyum Faisal , Guk Sergey , Chao Chingkong , Prahl Ulrich
Published: July 2022

Crystal plasticity-based numerical simulations help understand the local deformation behavior of multiphase materials. It is known that in full phase simulations, the local 2-dimensional (2D) representative volume elements (RVEs) results are distinctly different from 3-dimensional (3D) RVEs. In this work, the difference in the results of 2D and 3D RVEs is investigated systematically, and the effect of magnification, total strain and composition are analyzed. The 3D RVEs of dual-phase (DP)-steel are generated using DREAM-3D. The 2D RVEs are the sliced surfaces of corresponding 3D RVEs for a direct pixel-to-pixel comparison of results. It is shown that the corresponding 3D distribution can be rapidly derived from the 2D result based on the alternative error and least square method. The interactive parameters for these processes are identified and analyzed for the ferrite phase, which provides information about the convergence. Examined by qualitative and quantitative statistical analysis, it is shown that the corresponding 2D distribution by the fourth iteration has a prominent similarity with the exact 3D distribution. The work presented here contributes toward solving the paradox of comparing local strain from 2D crystal plasticity (CP) simulations with the effective 3D specimen used for tests.
High-Temperature Oxidation Behaviour of Duplex Fe-Mn-Al-Ni-C Lightweight Steel
Authors: Balaško Tilen , Šetina Batič Barbara , Medved Jožef , Burja Jaka
Published: July 2022

Lightweight Fe-Mn-Al-Ni-C steels are an attractive material, due to the combination of low density, high elongation, and yield strength. However, the high Al content is also interesting from the point of view of high-temperature resistance. High-temperature resistance is important for high-temperature applications and oxidation during heat treatment. Oxidation tests at 700, 800 and 900 °C were carried out to investigate the oxidation rates. Oxidation at 700 °C resulted in slight decarburisation, which was reflected in a slight weight loss in the thermogravimetric analysis. In contrast, the weight in the thermogravimetric analysis increased at 800 and 900 °C and the kinetics followed a parabolic law. The higher the temperature, the more weight is gained and the thicker the oxide layer becomes. The oxidation layer at 800 and 900 °C consisted mainly of hematite and magnetite, with minor amounts of wüstite, alumina and hercynite. Preferential oxidation of austenite was also observed, as it has an increased Mn content. In addition, ferrite had an increased content of Al and Ni, which provide additional oxidation resistance.
Real-World Clinical Outcomes after Genomic Profiling of Circulating Tumor DNA in Patients with Previously Treated Advanced Non-Small Cell Lung Cancer
Authors: Olsen Steven , Liao Jiemin , Hayashi Hidetoshi
Published: July 2022

Comprehensive genomic profiling for advanced non-small cell lung cancer (NSCLC) can identify patients for molecularly targeted therapies that improve clinical outcomes. We analyzed data from 3084 patients (median age 65 years, 72.9% with adenocarcinoma) with advanced NSCLC registered in a real-world healthcare claims database (GuardantINFORMTM, Guardant Health) who underwent next-generation sequencing (NGS)-based circulating tumor DNA (ctDNA) testing (Guardant360®, Guardant Health) after first-line therapy (28.0% with agents targeted against genomic alterations). ctDNA was detected in 2771 samples (89.9%), of which 41.9% harbored actionable alterations, most commonly EGFR (epidermal growth factor receptor) mutations (29.7%). Actionable alterations were detected in 26.7% of patients (534/2001) previously treated with non-targeted agents. Emerging potentially targetable mutations were found in 40.1% (309/770) of patients previously treated with targeted therapies. Among patients with qualifying alterations detected by ctDNA testing, the time to treatment discontinuation (median 8.8 vs. 4.2 months; hazard ratio 1.97, p < 0.001) and overall survival (median 36.1 vs. 16.6 months; hazard ratio 2.08, p < 0.001) were longer for those who received matched second-line therapy versus unmatched second-line therapy. In real-world practice, results of a blood-based NGS assay prior to second-line treatment inform therapeutic decisions that can improve clinical outcomes for patients with advanced NSCLC.
Genetic Characterization of Endangered Indian Mithun (Bos frontalis), Indian Bison/Wild Gaur (Bos gaurus) and Tho-tho Cattle (Bos indicus) Populations Using SSR Markers Reveals Their Diversity and Unique Phylogenetic Status
Authors: Mukherjee Sabyasachi , Mukherjee Anupama , Kumar Sanjeev , Verma Harendra , Bhardwaj Shivam , Togla Oshin , Joardar Siddhartha Narayan , Longkumer Imsusosang , Mech Moonmoon , Khate Kobu , Vupru Kezhavituo , Khan Meraj Haider , Kumar Suresh , Rajkhowa Chandan
Published: July 2022

Mithun (Bos frontalis) or gayal and Indian Bison or wild gaur (Bos gaurus) are listed among the rare and endangered bovine species of India. The remote location of mithun in four North Eastern Hill states (Arunachal Pradesh, Nagaland, Manipur, and Mizoram), scattered population size, and non-availability of genetic diversity status are major limitations towards devising a suitable breeding and conservation policy of these species. Since several studies have demonstrated the successful applicability of microsatellite/SSR markers across related genera/families in both crop plants and animal species, 30 FAO recommended cattle microsatellites were utilized for the assessment of the genetic diversity of Indian mithun, bison, and local Tho-tho cattle. Mitochondrial transmembrane protein coding cytochrome B (CYTB) complete sequence data of 71 bovine samples from India were also used to reinforce the study. Population structuring clustered the all bovines into three subgroups as per geographical location and species. Bottleneck analysis indicated a mode shift in the allelic frequency distribution of gaur, indicating minor genetic bottleneck events in the past, while no bottleneck was found in mithun and Tho-tho cattle. To our knowledge, this study represents the first report of molecular genetic characterization showing the population structure and status of genetic diversity in rare Indian bovines, namely, Mithun, Gaur, and Tho-tho cattle.
Diagnosis of Myelodysplastic Syndromes: From Immunological Observations to Clinical Applications
Authors: Simoni Yannick , Chapuis Nicolas
Published: July 2022

Myelodysplastic syndromes (MDS) constitute a very heterogeneous group of diseases with a high prevalence in elderly patients and a propensity for progression to acute myeloid leukemia. The complexity of these hematopoietic malignancies is revealed by the multiple recurrent somatic mutations involved in MDS pathogenesis and the paradoxical common phenotype observed in these patients characterized by ineffective hematopoiesis and cytopenia. In the context of population aging, the incidence of MDS will strongly increase in the future. Thus, precise diagnosis and evaluation of the progression risk of these diseases are imperative to adapt the treatment. Dysregulations of both innate and adaptive immune systems are frequently detected in MDS patients, and their critical role in MDS pathogenesis is now commonly accepted. However, different immune dysregulations and/or dysfunctions can be dynamically observed during the course of the disease. Monitoring the immune system therefore represents a new attractive tool for a more precise characterization of MDS at diagnosis and for identifying patients who may benefit from immunotherapy. We review here the current knowledge of the critical role of immune dysfunctions in both MDS and MDS precursor conditions and discuss the opportunities offered by the detection of these dysregulations for patient stratification.
Automated Classification of Atherosclerotic Radiomics Features in Coronary Computed Tomography Angiography (CCTA)
Authors: Yunus Mardhiyati Mohd , Mohamed Yusof Ahmad Khairuddin , Ab Rahman Muhd Zaidi , Koh Xue Jing , Sabarudin Akmal , Nohuddin Puteri N. E. , Ng Kwan Hoong , Kechik Mohd Mustafa Awang , Karim Muhammad Khalis Abdul
Published: July 2022

Radiomics is the process of extracting useful quantitative features of high-dimensional data that allows for automated disease classification, including atherosclerotic disease. Hence, this study aimed to quantify and extract the radiomic features from Coronary Computed Tomography Angiography (CCTA) images and to evaluate the performance of automated machine learning (AutoML) model in classifying the atherosclerotic plaques. In total, 202 patients who underwent CCTA examination at Institut Jantung Negara (IJN) between September 2020 and May 2021 were selected as they met the inclusion criteria. Three primary coronary arteries were segmented on axial sectional images, yielding a total of 606 volume of interest (VOI). Subsequently, the first order, second order, and shape order of radiomic characteristics were extracted for each VOI. Model 1, Model 2, Model 3, and Model 4 were constructed using AutoML-based Tree-Pipeline Optimization Tools (TPOT). The heatmap confusion matrix, recall (sensitivity), precision (PPV), F1 score, accuracy, receiver operating characteristic (ROC), and area under the curve (AUC) were analysed. Notably, Model 1 with the first-order features showed superior performance in classifying the normal coronary arteries (F1 score: 0.88; Inverse F1 score: 0.94), as well as in classifying the calcified (F1 score: 0.78; Inverse F1 score: 0.91) and mixed plaques (F1 score: 0.76; Inverse F1 score: 0.86). Moreover, Model 2 consisting of second-order features was proved useful, specifically in classifying the non-calcified plaques (F1 score: 0.63; Inverse F1 score: 0.92) which are a key point for prediction of cardiac events. Nevertheless, Model 3 comprising the shape-based features did not contribute to the classification of atherosclerotic plaques. Overall, TPOT shown promising capabilities in terms of finding the best pipeline and tailoring the model using CCTA-based radiomic datasets.
Anti-Carbamylated Protein (Anti-CarP) Antibodies in Patients Evaluated for Suspected Rheumatoid Arthritis
Authors: Ricchiuti Vincent , Chun Kelly Y. , Yang Jane M. , Aure Mary Ann , Gomez Luis , Norman Gary L. , Mahler Michael
Published: July 2022

(1) Background: Anti-carbamylated protein (CarP) antibodies have been studied as novel markers to aid in the diagnosis and prognosis of rheumatoid arthritis. (2) Methods: A total of 265 samples were included in the evaluation, for which 98 had results for anti-cyclic citrullinated peptide (CCP), 86 for rheumatoid factor (RF), and 212 for 14-3-3 eta protein. Anti-CarP antibodies were measured using a fetal calf serum-based single-step assay (research use only, Inova Diagnostics, San Diego, CA). (3) Results: Anti-CarP antibodies were significantly higher and more frequent in anti-CCP3.1+ (p = 0.0025), RF+ (p = 0.0043) and 14-3-3 eta+ (p = 0.028) samples compared to the negative counterpart group. In addition, isolated anti-CarP positivity occurred in samples negative for anti-CCP3.1, RF, or 14-3-3 eta. When anti-CarP antibodies were compared to each of the RF, anti-CCP3.1, and 14-3-3 eta by receiver operating characteristic (ROC) analyses, the area under the curve (AUC) values of 0.71 (RF), 0.68 (anti-CCP3.1), and 0.59 (14-3-3 eta), respectively, demonstrated a moderate correlation. Using an UpSet plot, we determined that 10.6% of the samples with available results for anti-CCP3.1, RF, and anti-CarP showed triple positivity. (4) Conclusions: Anti-carbamylated protein (anti-CarP) antibodies can be detected in anti-CCP, RF and 14-3-3 eta-positive and -negative patients, potentially identifying specific subsets of patients.
Myocardial Radiomics Texture Features Associated with Increased Coronary Calcium Score—First Results of a Photon-Counting CT
Authors: Ayx Isabelle , Tharmaseelan Hishan , Hertel Alexander , Nörenberg Dominik , Overhoff Daniel , Rotkopf Lukas T. , Riffel Philipp , Schoenberg Stefan O. , Froelich Matthias F.
Published: July 2022

The coronary artery calcium score is an independent risk factor of the development of adverse cardiac events. The severity of coronary artery calcification may influence the myocardial texture. Due to higher spatial resolution and signal-to-noise ratio, new CT technologies such as PCCT may improve the detection of texture alterations depending on the severity of coronary artery calcification. In this retrospective, single-center, IRB-approved study, left ventricular myocardium was segmented and radiomics features were extracted using pyradiomics. The mean and standard deviation with the Pearson correlation coefficient for correlations of features were calculated and visualized as boxplots and heatmaps. Random forest feature selection was performed. Thirty patients (26.7% women, median age 58 years) were enrolled in the study. Patients were divided into two subgroups depending on the severity of coronary artery calcification (Agatston score 0 and Agatston score ≥ 100). Through random forest feature selection, a set of four higher-order features could be defined to discriminate myocardial texture between the two groups. When including the additional Agatston 1–99 groups as a validation, a severity-associated change in feature intensity was detected. A subset of radiomics features texture alterations of the left ventricular myocardium was associated with the severity of coronary artery calcification estimated by the Agatston score.