bluuebunny/crossref_metadata_2025 huggingface dataset:
Sucrose Concentration and Fermentation Temperature Impact the Sensory Characteristics and Liking of Kombucha
Authors: Cohen Gil , Sela David A. , Nolden Alissa A.
Published: August 2023

Kombucha is a fermented tea beverage consumed for its probiotics and functional properties. It has a unique sensory profile driven by the properties of tea polyphenols and fermentation products, including organic acids. Fermentation temperature and sucrose content affect the fermentation process and the production of organic acids; yet less is known about their impacts on the sensory profile and consumer acceptance. Thus, we aimed to examine the impact of sucrose concentration and fermentation temperature on sensory attributes and liking. For this study, kombucha tea was fermented at three different concentrations of sucrose and fermented at two temperatures for 11 days. Fermentation was monitored by pH, brix, and titratable acidity, and consumers (n = 111) evaluated the kombucha for sensory attributes and overall liking. The fermentation temperature resulted in significant differences in titratable acidity, with higher temperatures producing more organic acids, resulting in higher astringency, and suppressed sweetness. The lower fermentation was reported as significantly more liked, with no difference in liking between the 7.5% and 10% sucrose kombucha samples. Fermentation temperature had the greatest impact on the sensory profile rather than sucrose concentration, which had a greater effect on the fermentation rate and production organic acids.
Advances in Genetic Tools and Their Application in Streptococcus thermophilus
Authors: Zhao Ruiting , Chen Zouquan , Liang Jie , Dou Jiaxin , Guo Fangyu , Xu Zhenshang , Wang Ting
Published: August 2023

Streptococcus thermophilus is a traditional starter. Nowadays, key aspects of S. thermophilus physiology have been revealed concerning the phenotypic traits relevant for industrial applications, including sugar metabolism, protein hydrolysis, and the production of important metabolites that affect the sensory properties of fermented foods as well as the original cooperation with Lactobacillus delbrueckii subsp. bulgaricus. Moreover, significant advances have been made in the synthetic biology toolbox of S. thermophilus based on technological advances in the genome and its sequencing and synthesis. In this review, we discuss the recently developed toolbox for S. thermophilus, including gene expression toolsets (promoters, terminators, plasmids, etc.) and genome editing tools. It can be used for both functionalized foods and therapeutic molecules for consumers. The availability of new molecular tools, including the genome editing toolbox, has facilitated the engineering of physiological studies of S. thermophilus and the generation of strains with improved technical and functional characteristics.
Effects of Low-Molecular-Weight Glutenin Subunit Encoded by Glu-A3 on Gluten and Chinese Fresh Noodle Quality
Authors: Zhou Hongwei , Zhang Yingquan , Yang Yanning , Zhang Yuyan , Ban Jinfu , Zhao Bo , Zhang Lei , Zhang Xiaoke , Guo Boli
Published: August 2023

Low-molecular-weight glutenin subunits (LMW-GS) account for 40% of the total wheat grain gluten protein fraction, which plays a significant role in the formation of noodle processing quality. The goal of this study was to clarify the effects of the major LMW-GS encoded by Glu-A3 on gluten and Chinese fresh noodle (CFN) quality. Four near-isogenic lines (NILs) were used as materials in this study, respectively carrying alleles Glu-A3a, Glu-A3b, Glu-A3c, and Glu-A3e, against the background of wheat variety Xiaoyan 22. The grain protein and its component contents and the gluten content, gluten index, farinograph properties, cooking quality, and textural quality of CFN were investigated. The results show that the ratios of glutenin to gliadin (Glu/Gli) in the NILs ranked them as Glu-A3b > Glu-A3c/Glu-A3a > Glu-A3e, and the unextractable polymeric protein content (UPP%), gluten index (GI), and farinograph quality in the NILs ranked them as Glu-A3b > Glu-A3c > Glu-A3a/Glu-A3e. Compared to Glu-A3b and Glu-A3a, the NILs carrying alleles Glu-A3c and Glu-A3e had better cooking and texture properties in CFN. All these findings suggest that the introduction of alleles Glu-A3c or Glu-A3e is an efficient method for quality improvement in CFN, which provides an excellent subunit selection for improving CFN quality.
Special Issue: Bioactive Compounds, Nutritional Quality, and Oxidative Stability of Edible Oils and By-Products of Their Extraction
Authors: Ratusz Katarzyna , Wroniak Małgorzata , Ścibisz Iwona
Published: August 2023

Edible oils (refined, virgin, and cold-pressed oils) are one of the most important components of the daily human diet and have a considerable influence on the proper functioning of our body [...]
VDR Immunohistochemistry Expression Is Down-Regulated in Colorectal Cells of Patients with IBD and Could Rank the Patients According to Their Complications Risk
Authors: Juniku-Shkololli Argjira , Manxhuka-Kërliu Suzana , Hamza Valon , Basholli Mimoza
Published: August 2023

Background: Rising incidence of inflammatory bowel disease (IBD) is an increasing concern among patients of young age worldwide and its most important complication is colitis-associated cancer (CAC). Vitamin D (VD) deficiency is common in IBD and inversely associated with disease activity; meanwhile, vitamin D receptor (VDR) signaling in the gut protects the mucosal epithelial barrier and inhibits inflammation in the colon. This study aims to investigate the connection between VDR expression and IBD in human colorectal tissues. Research design and methods: Using a cross-sectional analysis, this study investigated VDR nuclear immunohistochemistry expression in 35 subjects. The expression level was measured in patients with IBD, and compared with healthy controls (cut off 36.29%). Results: VDR nuclear expression was significantly down-regulated in colorectal tissues of patients with IBD, compared with controls (p = 0.025). Under-expression of VDR was more remarkable in colon cells of patients with UC (p = 0.023). These results confirm the protective role of VD for colonic mucosa in human colon as well, and suggest a benefit from VD supplementation in IBD patients. Conclusions: Our findings add to the body of evidence regarding the positive effect of VD in colorectal mucosal integrity. This study contributes in establishing one of the proposed markers related to disease activity, which can also predict the risk for complications.
The Potential Usefulness of the Expanded Carrier Screening to Identify Hereditary Genetic Diseases: A Case Report from Real-World Data
Authors: Veneruso Iolanda , Ranieri Annaluisa , Falcone Noemi , Tripodi Lorella , Scarano Carmela , La Monica Ilaria , Pastore Lucio , Lombardo Barbara , D’Argenio Valeria
Published: August 2023

Expanded carrier screening (ECS) means a comprehensive genetic analysis to evaluate an individual’s carrier status. ECS is becoming more frequently used, thanks to the availability of techniques such as next generation sequencing (NGS) and array comparative genomic hybridization (aCGH), allowing for extensive genome-scale analyses. Here, we report the case of a couple who underwent ECS for a case of autism spectrum disorder in the male partner family. aCGH and whole-exome sequencing (WES) were performed in the couple. aCGH analysis identified in the female partner two deletions involving genes associated to behavioral and neurodevelopment disorders. No clinically relevant alterations were identified in the husband. Interestingly, WES analysis identified in the male partner a pathogenic variant in the LPL gene that is emerging as a novel candidate gene for autism. This case shows that ECS may be useful in clinical contexts, especially when both the partners are analyzed before conception, thus allowing the estimation of their risk to transmit an inherited condition. On the other side, there are several concerns related to possible incidental findings and difficult-to-interpret results. Once these limits are defined by the establishment of specific guidelines, ECS may have a greater diffusion.
PSTPIP1-Associated Myeloid-Related Proteinemia Inflammatory (PAMI) Syndrome: A Systematic Review
Authors: Mejbri Manel , Renella Raffaele , Candotti Fabio , Jaques Cecile , Holzinger Dirk , Hofer Michael , Theodoropoulou Katerina
Published: August 2023

PSTPIP1 (proline-serine-threonine phosphatase-interactive protein 1)-associated myeloid-related proteinemia inflammatory (PAMI) syndrome, previously known as Hyperzincemia/Hypercalprotectinemia (Hz/Hc) syndrome, is a recently described, rare auto-inflammatory disorder caused by specific deleterious variants in the PSTPIP1 gene (p.E250K and p.E257K). The disease is characterized by chronic systemic inflammation, cutaneous and osteoarticular manifestations, hepatosplenomegaly, anemia, and neutropenia. Increased blood levels of MRP 8/14 and zinc distinguish this condition from other PSTPIP1-associated inflammatory diseases (PAID). The aim of this systematic review is to provide a comprehensive overview of the disease phenotype, course, treatment, and outcome based on reported cases. This systematic review adheres to the PRISMA guidelines (2020) for reporting. A literature search was performed in Embase, Medline, and Web of Science on 13 October 2022. The quality of the case reports and case series was assessed using the JBI checklists. Out of the 43 included patients with PAMI syndrome, there were 24 females and 19 males. The median age at onset was 3.9 years. The main clinical manifestations included anemia (100%), neutropenia (98%), cutaneous manifestations (74%), osteoarticular manifestations (72%), splenomegaly (70%), growth failure (57%), fever (51%), hepatomegaly (56%), and lymphadenopathy (39%). Systemic inflammation was described in all patients. Marked elevation of zinc and MRP 8/14 blood levels were observed in all tested patients. Response to treatment varied and no consistently effective therapy was identified. The most common therapeutic options were corticosteroids (N = 30), anakinra (N = 13), cyclosporine A (N = 11), canakinumab (N = 6), and anti-TNF (N = 14). Hematopoietic stem cell transplantation has been recently reported to be successful in five patients. Our review highlights the key characteristics of PAMI syndrome and the importance of considering this disease in the differential diagnosis of patients presenting with early-onset systemic inflammation and cytopenia.
Influence of Groundwater on the Very Shallow Geothermal Potential (vSGP) in the Area of a Large-Scale Geothermal Collector System (LSC)
Authors: Rammler Mario , Zeh Robin , Bertermann David
Published: August 2023

The water balance in the very shallow subsurface can be influenced by capillary rise due to a high groundwater table. Since moisture content is an important factor for the thermal conductivity of soils, this can also have an influence on the very shallow geothermal potential (vSGP). For this reason, the effect of spatial and seasonal variations in groundwater tables on moisture content in essential depth layers was investigated at a large-scale geothermal collector system (LSC) in Bad Nauheim, Germany. Quasi-one-dimensional simulations using the FEFLOW® finite-element simulation system were employed to determine site-dependent and seasonally varying moisture contents, from which thermal conductivities were derived. The model setup was previously validated based on recorded moisture contents. The simulations resulted in groundwater-related maximum seasonal and spatial differences in thermal conductivity of 0.14 W/(m∙K) in the LSC area. Larger differences of up to 0.21 W/(m∙K) resulted for different soil textures at the same depth due to different thermal properties. The results indicate that an efficient design of LSCs requires a sufficiently detailed subsurface exploration to account for small-scale variations in grain size distribution and groundwater level.
Predisposing Factors for Shallow Landslides in Alpine and Hilly/Apennines Environments: A Case Study from Piemonte, Italy
Authors: Fedato Eva , Fubelli Giandomenico , Kurilla Laurie , Tiranti Davide
Published: August 2023

Landslides are the most common natural hazard in the Piemonte region (northwestern Italy). This study is focused on shallow landslides caused by the sliding of the surficial detrital-colluvial cover caused by rainfall and characterized by a sudden and fast evolution. This study investigates shallow landslide events compared with variables considered as main predisposing qualitative factors (lithology, pedology and land use) to obtain a zonation of shallow landslide susceptibility in a GIS environment. Additionally, wildfire occurrence is also evaluated as a further predisposing factor for shallow landslide initiation. The resulting susceptibility map shows a strong correlation between the first three variables and shallow landslide occurrence, while it shows a negligible, or very localized, relationship with wildfire occurrence. Through the intersection of the predisposing factors with the landslide data points, a map of homogeneous zones is obtained; each identified zone is characterized by uniform lithological, soil-type, and land-use characteristics. The shallow landslide density occurrence is computed for each zone, resulting in a four-range susceptibility map. The resulting susceptibility zones can be used to define and evaluate the hazard linked to shallow landslide events for civil protection and regional planning purposes.
Link between Oral Health, Periodontal Disease, Smoking, and Systemic Diseases in Romanian Patients
Authors: Schwarz Christoph , Hajdu Adrian Ioan , Dumitrescu Ramona , Sava-Rosianu Ruxandra , Bolchis Vanessa , Anusca Diana , Hanghicel Andreea , Fratila Aurora Doris , Oancea Roxana , Jumanca Daniela , Galuscan Atena , Leretter Marius
Published: August 2023

(1) Background: The link between oral and systemic health is becoming increasingly obvious. Oral diseases, particularly periodontitis, have been linked to various diseases including diabetes and cardiovascular disease, among others. This survey aimed to assess the oral health condition of individuals, considering both their overall health and periodontal status, by performing oral examinations and collecting data using questionnaires. (2) Methods: After obtaining approval from the University’s Ethics Committee, the study was carried out from 2021 to 2022 at the Department of Oral Health, located in the Emergency Municipal Hospital in Timisoara, Timis County, Romania. Bivariate correlations were performed using nonparametric Spearman’s Rho using SPPS software version 23. To assess the importance of smoking frequency related to the severity of periodontitis diagnosis, the ANOVA Simple test (one-way) and Hochberg GT2 post hoc analysis were utilized. The chi-squared test was employed for nominal variables. A significance level of 0.05 (alpha = 0.05) was adopted for all statistical tests. (3) Results: There is a significant positive association between the frequency of systemic disease and the severity of the periodontitis diagnosis taken as a total, Rho (242) = 0.151, p < 0.05, and taken as a stage, Rho (242) = 0.199, p < 0.01, thus as the severity of the diagnosis increases, the patient presents comorbidities. Hochberg GT2 post hoc analysis indicates that the non-smoking group has statistically significantly lower diagnostic severity (Mdif = −0.81, p = 0.01), with a strong effect size (Cohen’s d = 0.73). (4) Conclusions: The findings are increasingly indicating a potential association between oral diseases and a range of systemic diseases. The impact of periodontal disease on the quality of life is significant, especially in individuals with associated systemic conditions and present risk factors.
Chemoprevention and Lifestyle Modifications for Risk Reduction in Sporadic and Hereditary Breast Cancer
Authors: Manna Eliza Del Fiol , Serrano Davide , Aurilio Gaetano , Bonanni Bernardo , Lazzeroni Matteo
Published: August 2023

Female breast cancer is the most commonly diagnosed malignancy worldwide. Risk assessment helps to identify women at increased risk of breast cancer and allows the adoption of a comprehensive approach to reducing breast cancer incidence through personalized interventions, including lifestyle modification, chemoprevention, intensified surveillance with breast imaging, genetic counseling, and testing. Primary prevention means acting on modifiable risk factors to reduce breast cancer occurrence. Chemoprevention with tamoxifen, raloxifene, anastrozole, and exemestane has already shown benefits in decreasing breast cancer incidence in women at an increased risk for breast cancer. For healthy women carrying BRCA 1 or BRCA 2 pathogenic/likely pathogenic (P/LP) germline variants, the efficacy of chemoprevention is still controversial. Adopting chemoprevention strategies and the choice among agents should depend on the safety profile and risk–benefit ratio. Unfortunately, the uptake of these agents has been low. Lifestyle modifications can reduce breast cancer incidence, and the recommendations for BRCA 1 or BRCA 2 P/LP germline variant carriers are comparable to the general population. This review summarizes the most recent evidence regarding the efficacy of chemoprevention and lifestyle interventions in women with sporadic and hereditary breast cancer.
Detection of Internal Browning Disorder in ‘Greensis’ Pears Using a Portable Non-Destructive Instrument
Authors: Seo Ho-Jin , Song Janghoon
Published: August 2023

Internal browning caused by prolonged cold storage poses a significant challenge to the visual appearance and flavor of Asian pears, which are economically valuable and a primary fruit exported from Korea. To address this issue, we established a cost-effective portable non-destructive piece of testing instrument using visible and near-infrared spectroscopy, focusing on the detection and discrimination of internal browning in ‘Greensis’ pears. Our investigation underscores the challenge of visually confirming browning, necessitating alternative methods for accurate assessment. Through comprehensive analysis involving three to four segments of 32 ‘Greensis’ pears, a robust calibration equation was derived. By employing partial least square regression on the absorption spectra within a 650–950 nm range, we developed a predictive model for detecting and quantifying browning. Through principal component analysis, normal pears were distinctly segregated from those exhibiting browning symptoms (discrimination accuracy of 95%). Furthermore, we established that pears with a browning index of 25 ± 2.0 are highly susceptible to browning following extended cold storage. Consequently, our proposed portable non-destructive instrument serves as a pivotal tool for farmers and fruit distributors, enabling efficient and precise selection of high-quality pears in an instance. Overall, our study introduces a practical solution to a pressing issue in the Asian pear industry.
In Silico Analysis, Anticonvulsant Activity, and Toxicity Evaluation of Schisandrin B in Zebrafish Larvae and Mice
Authors: Nieoczym Dorota , Banono Nancy Saana , Stępnik Katarzyna , Kaczor Agnieszka A. , Szybkowski Przemysław , Esguerra Camila Vicencio , Kukula-Koch Wirginia , Gawel Kinga
Published: August 2023

The aim of this study is to evaluate the anticonvulsant potential of schisandrin B, a main ingredient of Schisandra chinensis extracts. Schisandrin B showed anticonvulsant activity in the zebrafish larva pentylenetetrazole acute seizure assay but did not alter seizure thresholds in the intravenous pentylenetetrazole test in mice. Schisandrin B crosses the blood–brain barrier, which we confirmed in our in silico and in vivo analyses; however, the low level of its unbound fraction in the mouse brain tissue may explain the observed lack of anticonvulsant activity. Molecular docking revealed that the anticonvulsant activity of the compound in larval zebrafish might have been due to its binding to a benzodiazepine site within the GABAA receptor and/or the inhibition of the glutamate NMDA receptor. Although schisandrin B showed a beneficial anticonvulsant effect, toxicological studies revealed that it caused serious developmental impairment in zebrafish larvae, underscoring its teratogenic properties. Further detailed studies are needed to precisely identify the properties, pharmacological effects, and safety of schisandrin B.
Simultaneous Irradiation with UV-A, -B, and -C Lights Promotes Effective Decontamination of Planktonic and Sessile Bacteria: A Pilot Study
Authors: Bosso Andrea , Tortora Francesca , Culurciello Rosanna , Di Nardo Ilaria , Pistorio Valeria , Carraturo Federica , Colecchia Andrea , Di Girolamo Rocco , Cafaro Valeria , Notomista Eugenio , Ingenito Raffaele , Pizzo Elio
Published: August 2023

Surfaces in highly anthropized environments are frequently contaminated by both harmless and pathogenic bacteria. Accidental contact between these contaminated surfaces and people could contribute to uncontrolled or even dangerous microbial diffusion. Among all possible solutions useful to achieve effective disinfection, ultraviolet irradiations (UV) emerge as one of the most “Green” technologies since they can inactivate microorganisms via the formation of DNA/RNA dimers, avoiding the environmental pollution associated with the use of chemical sanitizers. To date, mainly UV-C irradiation has been used for decontamination purposes, but in this study, we investigated the cytotoxic potential on contaminated surfaces of combined UV radiations spanning the UV-A, UV-B, and UV-C spectrums, obtained with an innovative UV lamp never conceived so far by analyzing its effect on a large panel of collection and environmental strains, further examining any possible adverse effects on eukaryotic cells. We found that this novel device shows a significant efficacy on different planktonic and sessile bacteria, and, in addition, it is compatible with eukaryotic skin cells for short exposure times. The collected data strongly suggest this new lamp as a useful device for fast and routine decontamination of different environments to ensure appropriate sterilization procedures.
Quality Control Optimization for Minimizing Security Risks Associated with Mesenchymal Stromal Cell-Based Product Development
Authors: Rebelatto Carmen Lúcia Kuniyoshi , Boldrini-Leite Lidiane Maria , Daga Debora Regina , Marsaro Daniela Boscaro , Vaz Isadora May , Jamur Valderez Ravaglio , de Aguiar Alessandra Melo , Vieira Thalita Bastida , Furman Bianca Polak , Aguiar Cecília Oliveira , Brofman Paulo Roberto Slud
Published: August 2023

Mesenchymal stromal cells (MSCs) have been considered a therapeutic strategy in regenerative medicine because of their regenerative and immunomodulatory properties. The translation of MSC-based products has some challenges, such as regulatory and scientific issues. Quality control should be standardized and optimized to guarantee the reproducibility, safety, and efficacy of MSC-based products to be administered to patients. The aim of this study was to develop MSC-based products for use in clinical practice. Quality control assays include cell characterization, cell viability, immunogenicity, and cell differentiation; safety tests such as procoagulant tissue factor (TF), microbiological, mycoplasma, endotoxin, genomic stability, and tumorigenicity tests; and potency tests. The results confirm that the cells express MSC markers; an average cell viability of 96.9%; a low expression of HLA-DR and costimulatory molecules; differentiation potential; a high expression of TF/CD142; an absence of pathogenic microorganisms; negative endotoxins; an absence of chromosomal abnormalities; an absence of genotoxicity and tumorigenicity; and T-lymphocyte proliferation inhibition potential. This study shows the relevance of standardizing the manufacturing process and quality controls to reduce variability due to the heterogeneity between donors. The results might also be useful for the implementation and optimization of new analytical techniques and automated methods to improve safety, which are the major concerns related to MSC-based therapy.
Prospects of Using Chitosan-Based Biopolymers in the Treatment of Peripheral Nerve Injuries
Authors: Zhang Meng , An Heng , Zhang Fengshi , Jiang Haoran , Wan Teng , Wen Yongqiang , Han Na , Zhang Peixun
Published: August 2023

Peripheral nerve injuries are common neurological disorders, and the available treatment options, such as conservative management and surgical repair, often yield limited results. However, there is growing interest in the potential of using chitosan-based biopolymers as a novel therapeutic approach to treating these injuries. Chitosan-based biopolymers possess unique characteristics, including biocompatibility, biodegradability, and the ability to stimulate cell proliferation, making them highly suitable for repairing nerve defects and promoting nerve regeneration and functional recovery. Furthermore, these biopolymers can be utilized in drug delivery systems to control the release of therapeutic agents and facilitate the growth of nerve cells. This comprehensive review focuses on the latest advancements in utilizing chitosan-based biopolymers for peripheral nerve regeneration. By harnessing the potential of chitosan-based biopolymers, we can pave the way for innovative treatment strategies that significantly improve the outcomes of peripheral nerve injury repair, offering renewed hope and better prospects for patients in need.
Calcium Signaling during Cortical Apical Dendrite Initiation: A Role for Cajal-Retzius Neurons
Authors: Enck Joshua R. , Olson Eric C.
Published: August 2023

The apical dendrite of a cortical projection neuron (CPN) is generated from the leading process of the migrating neuron as the neuron completes migration. This transformation occurs in the cortical marginal zone (MZ), a layer that contains the Cajal-Retzius neurons and their axonal projections. Cajal-Retzius neurons (CRNs) are well known for their critical role in secreting Reelin, a glycoprotein that controls dendritogenesis and cell positioning in many regions of the developing brain. In this study, we examine the possibility that CRNs in the MZ may provide additional signals to arriving CPNs, that may promote the maturation of CPNs and thus shape the development of the cortex. We use whole embryonic hemisphere explants and multiphoton microscopy to confirm that CRNs display intracellular calcium transients of <1-min duration and high amplitude during early corticogenesis. In contrast, developing CPNs do not show high-amplitude calcium transients, but instead show a steady increase in intracellular calcium that begins at the time of dendritic initiation, when the leading process of the migrating CPN is encountering the MZ. The possible existence of CRN to CPN communication was revealed by the application of veratridine, a sodium channel activator, which has been shown to preferentially stimulate more mature cells in the MZ at an early developmental time. Surprisingly, veratridine application also triggers large calcium transients in CPNs, which can be partially blocked by a cocktail of antagonists that block glutamate and glycine receptor activation. These findings outline a model in which CRN spontaneous activity triggers the release of glutamate and glycine, neurotransmitters that can trigger intracellular calcium elevations in CPNs. These elevations begin as CPNs initiate dendritogenesis and continue as waves in the post-migratory cells. Moreover, we show that the pharmacological blockade of glutamatergic signaling disrupts migration, while forced expression of a bacterial voltage-gated calcium channel (CavMr) in the migrating neurons promotes dendritic growth and migration arrest. The identification of CRN to CPN signaling during early development provides insight into the observation that many autism-linked genes encode synaptic proteins that, paradoxically, are expressed in the developing cortex well before the appearance of synapses and the establishment of functional circuits.
The Role of Gender Differences and Menopause in Obesity-Related Renal Disease, Renal Inflammation and Lipotoxicity
Authors: Afonso-Alí Aaron , Porrini Esteban , Teixido-Trujillo Silvia , Pérez-Pérez José Antonio , Luis-Lima Sergio , Acosta-González Nieves Guadalupe , Sosa-Paz Irene , Díaz-Martín Laura , Rodríguez-González Covadonga , Rodríguez-Rodríguez Ana Elena
Published: August 2023

The pathogenesis of obesity-related-renal disease is unknown. Menopause can promote renal disease in obese women, but this interaction is unclear. In a previous study, we observed that obese male and female mice developed albuminuria, hyperfiltration, and glomerulomegaly, and these changes were more severe in those obese ovariectomized females. In this study, we also evaluated renal inflammation and lipotoxicity in that animal model. For six months, 43 males and 36 females C57BL6/J mice were randomized to standard diet (SD) or high fat diet (HFD). A group of female animals on SD or HFD was ovariectomized to simulate menopause. We evaluated cytokines: NF-κβ p65, IL-1β, MCP-1, TNF-α, total lipid content, lipid classes, and fatty acid profile in total lipid and individual lipid classes in renal tissue and urine. We found that obese males and females showed higher NF-kβ p-65, TNF-α and MCP-1 in renal tissue, and obese females ovariectomized had higher IL-1β and TNF-α compared with not-ovariectomized. Also, obese animals showed lower proinflammatory and higher anti-inflammatory fatty acids in kidney total lipids, while obese females ovariectomized had a more exacerbated pattern. In brief, obesity induces inflammation and an unbalanced lipidic profile in renal tissue. This pattern seems to be enhanced in obesity after menopause.
Predictive Role of Cytokine and Adipokine Panel in Hospitalized COVID-19 Patients: Evaluation of Disease Severity, Survival and Lung Sequelae
Authors: Bergantini Laura , d’Alessandro Miriana , Gangi Sara , Bianchi Francesco , Cameli Paolo , Perea Beatrice , Meocci Martina , Fabbri Gaia , Marrucci Sofia , Ederbali Moftah , Bargagli Elena
Published: August 2023

Coronavirus disease 2019 (COVID-19) may determine a multisystemic chronic syndrome after resolution of SARS-CoV-2 infection in a significant percentage of patients. Persistent cytokine dysregulation can contribute to long-lasting inflammation and tissue damage, resulting in the diverse, often debilitating symptoms experienced by some patients (so-called long COVID syndrome). The aim of our study was to evaluate the value of a panel of serum biomarkers of severity and prognosis in patients hospitalized for COVID-19 and also as predictive factors for the development of post-COVID lung sequelae after discharge from the hospital. All blood sampling was performed in the first 24 h after admission to the hospital. Serum analyte concentrations of IL-4, IL-2, CXCL10 (IP-10), IL-1β, TNF-α, CCL2 (MCP-1), IL-17A, IL-6, IL-10, IFN-γ, IL-12p70 and TGF-β1 were quantified by bead-based multiplex LEGENDplex™ analysis and commercially available ELISA kits. A total of 108 COVID-19 patients were enrolled in the study. Comparative analysis of these proteins showed higher levels of TGF-β and IL-6 and lower levels of RBP-4 and IL-10 in the severe group. Age, adiponectin, IL-8 and IL-32 resulted as the best predictors for survival. Moreover, IL-1β, IL17A, TNF-α, TGF-β, IL-4 and IL-6 were significantly higher in patients who showed HRCT evidence of fibrotic interstitial alterations at follow-up than patients who did not. The initial inflammatory status of patients on admission to the hospital with COVID-19, as reflected by the present panel of adipose tissue-related biomarkers and cytokines, offered insights into medium-term prognosis.
SET Domain Group 703 Regulates Planthopper Resistance by Suppressing the Expression of Defense-Related Genes
Authors: Wen Peizheng , He Jun , Zhang Qiong , Qi Hongzhi , Zhang Aoran , Liu Daoming , Sun Quanguang , Wang Yongsheng , Li Qi , Wang Wenhui , Chen Zhanghao , Wang Yunlong , Liu Yuqiang , Wan Jianmin
Published: August 2023

Plant defense responses against insect pests are intricately regulated by highly complex regulatory networks. Post-translational modifications (PTMs) of histones modulate the expression of genes involved in various biological processes. However, the role of PTMs in conferring insect resistance remains unclear. Through the screening of a T-DNA insertion activation-tagged mutant collection in rice, we identified the mutant planthopper susceptible 1 (phs1), which exhibits heightened expression of SET domain group 703 (SDG703). This overexpression is associated with increased susceptibility to the small brown planthopper (SBPH), an economically significant insect pest affecting rice crops. SDG703 is constitutively expressed in multiple tissues and shows substantial upregulation in response to SBPH feeding. SDG703 demonstrates the activity of histone H3K9 methyltransferase. Transcriptomic analysis revealed the downregulation of genes involved in effector-triggered immunity (ETI) and pattern-triggered immunity (PTI) in plants overexpressing SDG703. Among the downregulated genes, the overexpression of SDG703 in plants resulted in a higher level of histone H3K9 methylation compared to control plants. Collectively, these findings indicate that SDG703 suppresses the expression of defense-related genes through the promotion of histone methylation, consequently leading to reduced resistance against SBPH. The defense-related genes regulated by histone methylation present valuable targets for developing effective pest management strategies in future studies. Furthermore, our study provides novel insight into the epigenetic regulation involved in plant-insect resistance.
Unveiling the Link: Exploring Mitochondrial Dysfunction as a Probable Mechanism of Hepatic Damage in Post-Traumatic Stress Syndrome
Authors: Kondashevskaya Marina V. , Mikhaleva Lyudmila M. , Artem’yeva Kseniya A. , Aleksankina Valentina V. , Areshidze David A. , Kozlova Maria A. , Pashkov Anton A. , Manukhina Eugenia B. , Downey H. Fred , Tseilikman Olga B. , Yegorov Oleg N. , Zhukov Maxim S. , Fedotova Julia O. , Karpenko Marina N. , Tseilikman Vadim E.
Published: August 2023

PTSD is associated with disturbed hepatic morphology and metabolism. Neuronal mitochondrial dysfunction is considered a subcellular determinant of PTSD, but a link between hepatic mitochondrial dysfunction and hepatic damage in PTSD has not been demonstrated. Thus, the effects of experimental PTSD on the livers of high anxiety (HA) and low anxiety (LA) rats were compared, and mitochondrial determinants underlying the difference in their hepatic damage were investigated. Rats were exposed to predator stress for 10 days. Then, 14 days post-stress, the rats were evaluated with an elevated plus maze and assigned to HA and LA groups according to their anxiety index. Experimental PTSD caused dystrophic changes in hepatocytes of HA rats and hepatocellular damage evident by increased plasma ALT and AST activities. Mitochondrial dysfunction was evident as a predominance of small-size mitochondria in HA rats, which was positively correlated with anxiety index, activities of plasma transaminases, hepatic lipids, and negatively correlated with hepatic glycogen. In contrast, LA rats had a predominance of medium-sized mitochondria. Thus, we show links between mitochondrial dysfunction, hepatic damage, and heightened anxiety in PTSD rats. These results will provide a foundation for future research on the role of hepatic dysfunction in PTSD pathogenesis.
Inhibitory Effects of Simvastatin on IL-33-Induced MCP-1 via the Suppression of the JNK Pathway in Human Vascular Endothelial Cells
Authors: Umebashi Katsuyuki , Yamamoto Masayoshi , Tokito Akinori , Sudou Ku , Takenoshita Yoko , Jougasaki Michihisa
Published: August 2023

An alarmin, interleukin (IL)-33 is a danger signal that causes inflammation, inducing chemotactic proteins such as monocyte chemoattractant protein (MCP)-1 in various cells. As statins have pleiotropic actions including anti-inflammatory properties, we investigated the effects of simvastatin on IL-33-induced MCP-1 expression in human umbilical vein endothelial cells (HUVECs). HUVECs were stimulated with IL-33 in the presence or absence of simvastatin. Gene expression and protein secretion of MCP-1, phosphorylation of mitogen-activated protein kinase (MAPK), nuclear translocation of phosphorylated c-Jun, and human monocyte migration were investigated. Immunocytochemical staining and Western immunoblot analysis revealed that IL-33 augmented MCP-1 protein expression in HUVECs. Real-time reverse transcription–polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) showed that IL-33 significantly increased MCP-1 mRNA and protein secretion, which were suppressed by c-jun N-terminal kinase (JNK) inhibitor SP600125 and p38 MAPK inhibitor SB203580. Simvastatin inhibited IL-33-induced MCP-1 mRNA, protein secretion, phosphorylation of JNK and c-Jun. Additionally, the IL-33-induced nuclear translocation of phosphorylated c-Jun and THP-1 monocyte migration were also blocked by simvastatin. This study demonstrated that IL-33 induces MCP-1 expression via the JNK and p38 MAPK pathways in HUVECs, and that simvastatin inhibits MCP-1 production by selectively suppressing JNK. Simvastatin may inhibit the progression of IL-33-induced inflammation via suppressing JNK to prevent MCP-1 production.
Expression of EMP 1, 2, and 3 in Adrenal Cortical Neoplasm and Pheochromocytoma
Authors: Cha Yoon Jin , Koo Ja Seung
Published: August 2023

The purpose of this study is to investigate the expression of the epithelial membrane proteins (EMP) 1, 2, and 3 in adrenal gland neoplasm and to explore the broader implications of this. Tissue microarrays were constructed for 132 cases of adrenal cortical neoplasms (ACN) (adrenal cortical adenoma (115 cases), and carcinoma (17 cases)) and 189 cases of pheochromocytoma. Immunohistochemical staining was performed to identify EMP 1, 2, and 3, and was compared with clinicopathological parameters. The H-score of EMP 3 (p < 0.001) was higher in pheochromocytoma when compared to that of ACN, and the H-score of EMP 1 (p < 0.001) and EMP 3 (p < 0.001) was higher in adrenal cortical carcinomas when compared to that of adrenal cortical adenomas. A higher EMP 1 H-score was observed in pheochromocytomas with a GAPP score ≥3 (p = 0.018). In univariate analysis, high levels of EMP 1 and EMP 3 expression in ACN were associated with shorter overall survival (p = 0.001). Differences were observed in the expression of EMPs between ACN and pheochromocytoma. EMPs are associated with malignant tumor biology in adrenal cortical neoplasm and pheochromocytoma, suggesting the role of a prognostic and/or predictive factor for EMPs in adrenal tumor.
Research Progress of Macromolecules in the Prevention and Treatment of Sepsis
Authors: Su Jingqian , Wu Shun , Zhou Fen , Tong Zhiyong
Published: August 2023

Sepsis is associated with high rates of mortality in the intensive care unit and accompanied by systemic inflammatory reactions, secondary infections, and multiple organ failure. Biological macromolecules are drugs produced using modern biotechnology to prevent or treat diseases. Indeed, antithrombin, antimicrobial peptides, interleukins, antibodies, nucleic acids, and lentinan have been used to prevent and treat sepsis. In vitro, biological macromolecules can significantly ameliorate the inflammatory response, apoptosis, and multiple organ failure caused by sepsis. Several biological macromolecules have entered clinical trials. This review summarizes the sources, efficacy, mechanism of action, and research progress of macromolecular drugs used in the prevention and treatment of sepsis.
Curcumin-Loaded Nanofibrous Matrix Accelerates Fibroblast Cell Proliferation and Enhances Wound Healing via GSK3-β Inhibition
Authors: Konain Kiran , Saddique Nayyer , Samie Muhammad , Rahman Zia , Farid Sajida , Hameed Shazia , Mirza Munazza , Wu Wenhui , Woo Kyung , Arany Praveen , Rahman Saeed
Published: August 2023

Wound healing is a multifaceted biological process influenced by both intrinsic and extrinsic factors. The ability of Wnt signaling to activate cell proliferation appears to serve a central role in wound healing. Therefore, the direct activation of Wnt or inhibition of the Wnt antagonist could be an ideal approach for the stimulation of wound healing. This study aimed to investigate the underlying mechanism of small molecule-loaded nanofibrous matrix in inducing wound healing. Herein, a naturally derived small molecule, curcumin, was used to inhibit the GSK3-β, which is considered a negative regulator of the Wnt/β-catenin signaling pathway. The docking results demonstrated that curcumin makes a complex with GSK3-β at seven specific sites, thereby inhibiting its activity. Moreover, the stabilization of β-catenin appeared to be increased with the treatment of curcumin. Next, curcumin was incorporated in poly ε-caprolactone nanofibrous matrices for controlled–sustained drug release to induce cell function. Curcumin-loaded nanofibrous matrix not only enhanced fibroblast cell proliferation, but also induced the expression of the fibroblast growth factor (FGF) in vitro. Moreover, the in vivo results showed that these nanofibrous mats significantly induced wound closure in 12 mm critical-sized defect. Collectively, these results suggest that the developed nanofibrous matrix promotes impaired wound healing by modulating cell proliferation and enhancing FGF expression that promotes wound closure.
Fit-for-Purpose Information for Offshore Wind Farming Applications—Part-I: Identification of Needs and Solutions
Authors: She Jun , Blauw Anouk , Laakso Lauri , Mourre Baptiste , Schulz-Stellenfleth Johannes , Wehde Henning
Published: August 2023

The rapid expansion of offshore wind farms (OWFs) in European seas is accompanied by many challenges, including efficient and safe operation and maintenance, environmental protection, and biodiversity conservation. Effective decision-making for industry and environmental agencies relies on timely, multi-disciplinary marine data to assess the current state and predict the future state of the marine system. Due to high connectivity in space (land–estuarial–coastal sea), socioeconomic (multi-sectoral and cross-board), and environmental and ecological processes in sea areas containing OWFs, marine observations should be fit for purpose in relation to multiple OWF applications. This study represents an effort to map the major observation requirements (Part-I), identify observation gaps, and recommend solutions to fill those gaps (Part-II) in order to address multi-dimension challenges for the OWF industry. In Part-I, six targeted areas are selected, including OWF operation and maintenance, protection of submarine cables, wake and lee effects, transport and security, contamination, and ecological impact assessments. For each application area, key information products are identified, and integrated modeling–monitoring solutions for generating the information products are proposed based on current state-of-the-art methods. The observation requirements for these solutions, in terms of variables and spatial and temporal sampling needs, are therefore identified.
A Modulation Recognition System for Underwater Acoustic Communication Signals Based on Higher-Order Cumulants and Deep Learning
Authors: Zhang Run , He Chengbing , Jing Lianyou , Zhou Chaopeng , Long Chao , Li Jiachao
Published: August 2023

Underwater acoustic channels, influenced by time-varying, space-varying, frequency-varying, and multipath effects, pose significant interference challenges to underwater acoustic communication (UWAC) signals, especially in non-cooperative scenarios. The task of modulating and identifying distorted signals faces huge challenges. Although traditional modulation recognition methods can be useful in the radio field, they often prove inadequate in underwater environments. This paper introduces a modulation recognition system for recognizing UWAC signals based on higher-order cumulants and deep learning. The system achieves blind recognition of received UWAC signals even under non-cooperative conditions. Higher-order cumulants are employed due to their excellent noise resistance, enabling the differentiation of OFDM signals from PSK and FSK signals. Additionally, the high-order spectra differences among signals are utilized for the intra-class recognition of PSK and FSK signals. Both simulation and lake test results substantiate the effectiveness of the proposed method.
Exploring the Advantages of Using Social Media in the Romanian Retail Sector
Authors: Vasiliu Cristinel , Felea Mihai , Albastroiu Nastase Irina , Bucur Mihaela , Istrate-Scradeanu Adrian
Published: August 2023

The emergence of social media led to major changes in the manner in which retailers accomplish their daily profession, particularly since they provide traders with platforms for business development and brand improvement. In spite of this, little is known about their impact and influence on retail businesses. Research on retailers’ perceptions concerning social media is scarce and fragmented, which justifies the current increasing focus of scholars and practitioners on this subject. In this study, a quantitative research design was utilized, aiming to identify the advantages of social media as perceived by retailers in Romania. The findings confirm the hypotheses, acknowledging that Romanian retailers perceive social media as offering great advantages for individuals employed in the retail sector. The practical implications of our research were grouped according to the analyzed aspects, as follows: gathering information, content creation, and customer communication, approached as advantages of adopting social media in retail. This study contributes to the limited literature on social media and the perceived advantages of Romanian retailers, which has implications for further research in this field of knowledge.
Performance Analysis of High-Performance Concrete Materials in Civil Construction
Authors: Han Yongguang , Zhou Tianhua
Published: August 2023

This paper develops the mechanical and durable samples of C50 high-performance concrete, studies the mechanical properties, crack resistance, sulfate attack resistance, frost resistance, and impermeability of concrete with different mineral admixtures of mineral powder and fly ash, and obtains the best mineral admixture of mineral powder and fly ash to improve the performance of high-performance concrete. The results show that the doping effect is the best when the ratio of prepared mineral powder to fly ash is 3:2. With the increase in the mineral powder–fly ash admixture, the slump and expansion of high-performance concrete decrease rapidly at first and then slowly. In total, 60% doping is the turning point; the compressive and flexural strengths of concrete decreased slowly at first and then rapidly. Taking 30% of the admixture as the turning point, 35% of the mineral powder fly ash is generally selected. By mixing and adding a certain proportion of fly ash and mineral powder admixtures, the crack resistance of concrete is enhanced, and the shrinkage and cracking are reduced. The corrosion resistance coefficient will exceed 88%, the relative dynamic elastic modulus will exceed 95%, and the impermeability grade will reach P17. The durability of concrete can be improved by adding mineral admixtures.