bluuebunny/crossref_metadata_2025 huggingface dataset:
Map of Enteropathogenic Escherichia coli Targets Mitochondria and Triggers DRP-1-Mediated Mitochondrial Fission and Cell Apoptosis in Bovine Mastitis
Authors: Li Yanan , Zhu Yaohong , Chu Bingxin , Liu Ning , Chen Shiyan , Wang Jiufeng , Zou Yunjing
Published: April 2022

Bovine mastitis seriously affects bovine health and dairy product quality. Escherichia coli is the most important pathogen in the environment and dairy products. Enteropathogenic Escherichia coli (EPEC) is a zoonotic pathogen, which seriously threatens the health of people and dairy cows. We recently reported that E. coli can induce endogenous apoptosis in bovine mammary epithelial cells. However, the mechanism of EPEC-damaged mitochondria and -induced bovine mastitis is unclear. In this study, we found that EPEC can induce DRP-1-dependent mitochondrial fission and apoptosis. This was verified by the application of Mdivi, a DRP-1 inhibitor. Meanwhile, in order to verify the role of the Map virulence factor in EPEC-induced bovine mastitis, we constructed a map mutant, complementary strain, and recombinant plasmid MapHis. In the present study, we find that Map induced DRP-1-mediated mitochondrial fission, resulting in mitochondrial dysfunction and apoptosis. These inferences were further verified in vivo by establishing a mouse mastitis model. After the map gene was knocked out, breast inflammation and apoptosis in mice were significantly alleviated. All results show that EPEC targets mitochondria by secreting the Map virulence factor to induce DRP-1-mediated mitochondrial fission, mitochondrial dysfunction, and endogenous apoptosis in bovine mastitis.
Mechanisms and Clinical Implications of Endothelial Dysfunction in Arterial Hypertension
Authors: Ambrosino Pasquale , Bachetti Tiziana , D’Anna Silvestro Ennio , Galloway Brurya , Bianco Andrea , D’Agnano Vito , Papa Antimo , Motta Andrea , Perrotta Fabio , Maniscalco Mauro
Published: April 2022

The endothelium is composed of a monolayer of endothelial cells, lining the interior surface of blood and lymphatic vessels. Endothelial cells display important homeostatic functions, since they are able to respond to humoral and hemodynamic stimuli. Thus, endothelial dysfunction has been proposed as a key and early pathogenic mechanism in many clinical conditions. Given the relevant repercussions on cardiovascular risk, the complex interplay between endothelial dysfunction and systemic arterial hypertension has been a matter of study in recent years. Numerous articles have been published on this issue, all of which contribute to providing an interesting insight into the molecular mechanisms of endothelial dysfunction in arterial hypertension and its role as a biomarker of inflammation, oxidative stress, and vascular disease. The prognostic and therapeutic implications of endothelial dysfunction have also been analyzed in this clinical setting, with interesting new findings and potential applications in clinical practice and future research. The aim of this review is to summarize the pathophysiology of the relationship between endothelial dysfunction and systemic arterial hypertension, with a focus on the personalized pharmacological and rehabilitation strategies targeting endothelial dysfunction while treating hypertension and cardiovascular comorbidities.
Prolonged Mechanical Ventilation: Outcomes and Management
Authors: Huang Hung-Yu , Huang Chih-Yu , Li Li-Fu
Published: April 2022

The number of patients requiring prolonged mechanical ventilation (PMV) is increasing worldwide, placing a burden on healthcare systems. Therefore, investigating the pathophysiology, risk factors, and treatment for PMV is crucial. Various underlying comorbidities have been associated with PMV. The pathophysiology of PMV includes the presence of an abnormal respiratory drive or ventilator-induced diaphragm dysfunction. Numerous studies have demonstrated that ventilator-induced diaphragm dysfunction is related to increases in in-hospital deaths, nosocomial pneumonia, oxidative stress, lung tissue hypoxia, ventilator dependence, and costs. Thus far, the pathophysiologic evidence for PMV has been derived from clinical human studies and experimental studies in animals. Moreover, recent studies have demonstrated the outcome benefits of pharmacological agents and rehabilitative programs for patients requiring PMV. However, methodological limitations affected these studies. Controlled prospective studies with an adequate number of participants are necessary to provide evidence of the mechanism, prognosis, and treatment of PMV. The great epidemiologic impact of PMV and the potential development of treatment make this a key research field.
Platelet-to-White Blood Cell Ratio Is Associated with Adverse Outcomes in Cirrhotic Patients with Acute Deterioration
Authors: Kim Jung Hee , Kim Sung-Eun , Song Do-Seon , Kim Hee-Yeon , Yoon Eileen , Kim Tae-Hyung , Jung Young-Kul , Suk Ki Tae , Jun Baek-Gyu , Yim Hyung-Joon , Kwon Jung-Hyun , Lee Sung-Won , Kang Seong-Hee , Kim Moon-Young , Jeong Soung-Won , Jang Jae-Young , Yoo Jeong-Ju , Kim Sang-Gyune , Jin Young-Joo , Cheon Gab-Jin , Kim Byung-Seok , Seo Yeon Seok , Kim Hyung-Su , Sinn Dong-Hyun , Chung Woo-Jin , Kim Hwi Young , Lee Han Ah , Nam Seung-Woo , Kim In-Hee , Suh Jung-Il , Kim Ji-Hoon , Chae Hee-Bok , Sohn Joo-Hyun , Cho Ju-Yeon , Kim Yoon-Jun , Yang Jin-Mo , Park Jung-Gil , Kim Won , Cho Hyun-Chin , Kim Dong Joon
Published: April 2022

Background: The platelet-to-white blood cell ratio (PWR) is a hematologic marker of the systemic inflammatory response. Recently, the PWR was revealed to have a role as an independent prognostic factor for mortality in patients with hepatitis B virus (HBV)-related acute-on-chronic failure (ACLF) and HBV-related liver cirrhosis (LC) with acute decompensation (AD). However, the prognostic role of the PWR still needs to be investigated in LC patients with AD. In this study, we analyzed whether the PWR could stratify the risk of adverse outcomes (death or liver transplantation (LT)) in these patients. Methods: A prospective cohort of 1670 patients with AD of liver cirrhosis ((age: 55.2 ± 7.8, male = 1226 (73.4%)) was enrolled and evaluated for 28-day and overall adverse outcomes. Results: During a median follow-up of 8.0 months (range, 1.9–15.5 months), 424 (25.4%) patients had adverse outcomes (death = 377, LT = 47). The most common etiology of LC was alcohol use (69.7%). The adverse outcome rate was higher for patients with a PWR ≤ 12.1 than for those with a PWR > 12.1. A lower PWR level was a prognostic factor for 28-day adverse outcomes (PWR: hazard ratio 1.707, p = 0.034) when adjusted for the etiology of cirrhosis, infection, ACLF, and the MELD score. In the subgroup analysis, the PWR level stratified the risk of 28-day adverse outcomes regardless of the presence of ACLF or the main form of AD but not for those with bacterial infection. Conclusions: A lower PWR level was associated with 28-day adverse outcomes, indicating that the PWR level can be a useful and simple tool for stratifying the risk of 28-day adverse outcomes in LC patients with AD.
Classifying Pituitary Adenoma Invasiveness Based on Radiological, Surgical and Histological Features: A Retrospective Assessment of 903 Cases
Authors: Lu Liang , Wan Xueyan , Xu Yu , Chen Juan , Shu Kai , Lei Ting
Published: April 2022

Invasiveness is a major predictor of surgical outcome and long-term prognosis in patients with pituitary adenomas (PAs). We assessed PA invasiveness via radiological, surgical and histological perspectives to establish a classification scheme for predicting invasive behavior and poor prognosis. We retrospectively analyzed 903 patients who underwent transnasal-transsphenoidal surgery between January 2013 and December 2019. Radiological (hazard ratio (HR) 5.11, 95% confidence interval (CI): 3.98–6.57, p < 0.001) and surgical (HR 6.40, 95% CI: 5.09–8.06, p < 0.001) invasiveness better predicted gross-total resection (GTR) and recurrence/progression-free survival (RPFS) rates than did histological invasiveness (HR 1.44, 95% CI: 1.14–1.81, p = 0.003). Knosp grades 2 (HR 4.63, 95% CI: 2.13–10.06, p < 0.001) and 3 (HR 2.23, 95% CI: 1.39–3.59, p = 0.011) with surgical invasiveness were better predictors of prognosis than corresponding Knosp grades without surgical invasiveness. Classifications 1 and 2 were established based on radiological, surgical and histological invasiveness, and Knosp classification and surgical invasiveness, respectively. Classification 2 predicted RPFS better than Knosp classification and Classification 1. Overall, radiological and surgical invasiveness were clinically valuable as prognostic predictors. The convenience and good accuracy of Invasiveness in Classification 2 is useful for identifying invasive PAs and facilitating the development of treatment plans.
Comparative Diagnostic Accuracy of the STANDARD M10 Assay for the Molecular Diagnosis of SARS-CoV-2 in the Point-of-Care and Critical Care Settings
Authors: Domnich Alexander , Orsi Andrea , Trombetta Carlo-Simone , Costa Elisabetta , Guarona Giulia , Lucente Miriana , Ricucci Valentina , Bruzzone Bianca , Icardi Giancarlo
Published: April 2022

Accurate and rapid molecular diagnosis of COVID-19 is a crucial step to tackle the ongoing pandemic. The primary objective of this study was to estimate the real-world performance of the novel RT-PCR STANDARD M10 SARS-CoV-2 assay in a large number of nasopharyngeal (NP) specimens eluted in universal transport medium. The secondary objective was to evaluate the compatibility of this kit in testing NP samples eluted in an inactivated transport medium (essential for point-of-care testing) and lower respiratory tract (LRT) specimens, which are commonly collected in critical care. A total of 591 samples were analyzed. Compared with the standard extraction-based RT-PCR Allplex 2019-nCoV (time-to-result of 270 min), the sensitivities of the STANDARD M10 were 100% (95% CI: 98.1–100%), 95.5% (95% CI: 91.7–97.6%), and 99.5% (95% CI: 97.2–99.9%) for ≥1 gene, the ORF1ab gene, and the E gene, respectively, while the specificity was 100% (95% CI: 98.7–100%). The diagnostic accuracy was 100% in testing both NP samples eluted in an inactivated transport medium and LRT specimens. STANDARD M10 reliably detects SARS-CoV-2 in 60 min, may be used as a POC tool, and is suitable for testing LRT specimens in the critical care setting.
Forecasting Shanghai Container Freight Index: A Deep-Learning-Based Model Experiment
Authors: Hirata Enna , Matsuda Takuma
Published: April 2022

With the increasing availability of large datasets and improvements in prediction algorithms, machine-learning-based techniques, particularly deep learning algorithms, are becoming increasingly popular. However, deep-learning algorithms have not been widely applied to predict container freight rates. In this paper, we compare a long short-term memory (LSTM) method and a seasonal autoregressive integrated moving average (SARIMA) method for forecasting the comprehensive and route-based Shanghai Containerized Freight Index (SCFI). The research findings indicate that the LSTM deep learning models outperformed SARIMA models in most of the datasets. For South America and the east coast of the U.S. routes, LSTM could reduce forecasting errors by as much as 85% compared to SARIMA. The SARIMA models performed better than LSTM in predicting freight movements on the west and east Japan routes. The study contributes to the literature in four ways. First, it presents insights for improving forecasting accuracy. Second, it helps relevant parties understand the trends of container freight markets for wiser decision-making. Third, it helps relevant stakeholders understand overall container shipping market trends. Lastly, it can help hedge against the volatility of freight rates.
New Antibacterial Chloro-Containing Polyketides from the Alga-Derived Fungus Asteromyces cruciatus KMM 4696
Authors: Zhuravleva Olesya I. , Oleinikova Galina K. , Antonov Alexandr S. , Kirichuk Natalia N. , Pelageev Dmitry N. , Rasin Anton B. , Menshov Alexander S. , Popov Roman S. , Kim Natalya Yu. , Chingizova Ekaterina A. , Chingizov Artur R. , Volchkova Olga O. , von Amsberg Gunhild , Dyshlovoy Sergey A. , Yurchenko Ekaterina A. , Guzhova Irina V. , Yurchenko Anton N.
Published: April 2022

Six new polyketides acrucipentyns A–F (1–6) were isolated from the alga-derived fungus Asteromyces cruciatus KMM 4696. Their structures were established based on spectroscopic methods. The absolute configurations of acrucipentyn A was assigned by the modified Mosher’s method and ROESY data analysis. Acrucipentyns A–E were identified to be the very first examples of chlorine-containing asperpentyn-like compounds. The cytotoxic and antimicrobial activities of the isolated compounds were examined. Acrucipentyns A–F were found as antimicrobial agents, which inhibited sortase A enzyme activity, bacterial growth and biofilm formation of Staphylococcus aureus and decreased LDH release from human keratinocytes HaCaT in S. aureus skin infection in an in vitro model.
Soil Compaction Prevention, Amelioration and Alleviation Measures Are Effective in Mechanized and Smallholder Agriculture: A Meta-Analysis
Authors: Yang Peipei , Dong Wenxu , Heinen Marius , Qin Wei , Oenema Oene
Published: April 2022

Background: The compaction of subsoils in agriculture is a threat to soil functioning. Measures aimed at the prevention, amelioration, and/or impact alleviation of compacted subsoils have been studied for more than a century, but less in smallholder agriculture. Methods: A meta-analysis was conducted to quantitatively examine the effects of the prevention, amelioration, and impact alleviation measures in mechanized and small-holder agriculture countries, using studies published during 2000~2019/2020. Results: Mean effect sizes of crop yields were large for controlled traffic (+34%) and irrigation (+51%), modest for subsoiling, deep ploughing, and residue return (+10%), and negative for no-tillage (−6%). Mean effect sizes of soil bulk density were small (<10%), suggesting bulk density is not a sensitive ‘state’ indicator. Mean effect sizes of penetration resistance were relatively large, with large variations. Controlled traffic had a larger effect in small-holder farming than mechanized agriculture. Conclusion: We found no fundamental differences between mechanized and smallholder agriculture in the mean effect sizes of the prevention, amelioration, and impact alleviation measures. Measures that prevent soil compaction are commonly preferred, but amelioration and alleviation are often equally needed and effective, depending on site-specific conditions. A toolbox of soil compaction prevention, amelioration, and alleviation measures is needed, for both mechanized and smallholder agriculture.
Protein–Protein Interaction (PPI) Network of Zebrafish Oestrogen Receptors: A Bioinformatics Workflow
Authors: Zainal-Abidin Rabiatul-Adawiah , Afiqah-Aleng Nor , Abdullah-Zawawi Muhammad-Redha , Harun Sarahani , Mohamed-Hussein Zeti-Azura
Published: April 2022

Protein–protein interaction (PPI) is involved in every biological process that occurs within an organism. The understanding of PPI is essential for deciphering the cellular behaviours in a particular organism. The experimental data from PPI methods have been used in constructing the PPI network. PPI network has been widely applied in biomedical research to understand the pathobiology of human diseases. It has also been used to understand the plant physiology that relates to crop improvement. However, the application of the PPI network in aquaculture is limited as compared to humans and plants. This review aims to demonstrate the workflow and step-by-step instructions for constructing a PPI network using bioinformatics tools and PPI databases that can help to predict potential interaction between proteins. We used zebrafish proteins, the oestrogen receptors (ERs) to build and analyse the PPI network. Thus, serving as a guide for future steps in exploring potential mechanisms on the organismal physiology of interest that ultimately benefit aquaculture research.
Research and Optimization of Process Parameters for Internal Thread Forming Based on Numerical Simulation and Experimental Analysis
Authors: He Qiang , Jiang Yuxiang , Jing Xuwen , Jiang Yonggang , Zhou Honggen , Fu Bofeng
Published: April 2022

In order to improve the forming quality of extruded thread, finite element analysis and experimental research are combined to reduce the two keys that affect thread quality in the machining process—extrusion torque and extrusion temperature. The effects of different processing parameters on the extrusion torque and temperature are obtained by numerical simulation, including the bottom hole diameter of the workpiece, the machine tool speed, and the lubrication medium. For the purpose of reducing extrusion torque and temperature, the process parameters for internal thread forming are further optimized by orthogonal design. It is determined that when machining the M22 × 2 internal thread on the connecting rod of the marine diesel engine made of 42CrMo4 steel, the bottom hole diameter of the workpiece should be 21.20 mm, the speed of the machine tool should be 40 RPM, and the lubricating medium should be PDMS polydimethylsiloxane coolant. Compared to before optimization, the maximum extrusion torque and the maximum extrusion temperature are reduced by 19.27% and 15.07%, respectively. On the premise of ensuring the thread connection strength, the height of the thread tooth is reduced by 0.052 mm, and the surface condition of the thread is improved. The surface microhardness at the root, top, and side of the thread increases by about 5 HV0.2, and the depth of the hardened layer increases by 0.05 mm. The results show that the quality of the optimized thread is higher.
A Fault Diagnosis Model for Coaxial-Rotor Unit Using Bidirectional Gate Recurrent Unit and Highway Network
Authors: Peng Zhaoqin , Dong Kunyu , Wang Yan , Huang Xucong
Published: April 2022

A turbojet engine is the most significant part of an Internal Combustion Engine (ICE) for Hybrid Electric Vehicles. Specifically, the coaxial-rotor unit is the key component, whose performance largely affects the working efficiency. Thereby, the fault diagnosis methods for coaxial-rotor units is a main focus. In line with our test results, the bearing circlip is the most vulnerable element while rotating. Moreover, the low-speed rotating fault diagnosis is even challenging for current methods. Since the fault diagnosis on the bearing circlip of coaxial-rotor units is absent, this paper establishes a test rig on a running coaxial-rotor unit under different working conditions. The three-directional vibration signals are collected and analyzed to demonstrate the working states. On the task of bearing circlip failure classification, a deep-learning-based model using the Bidirectional Gate Recurrent Unit and the Highway Network is developed, which is capable of capturing hidden features and removing unrelated information. For working performance evaluation, experiments on the data of different rotating speeds are carried out. Among all the fault diagnosis methods, our model is the best approach and achieves an average accuracy of 99.4%. The encouraging results reveal that the proposed model is effective in both the high-speed and low-speed fault diagnosis of bearing circlip malfunction.
A Novel Design of an Inner Rotor for Optimizing the Air-Gap Magnetic Field of Hollow-Cup Motors
Authors: Sun Jinji , Ren Jianyi , Sun Haoxi
Published: April 2022

In order to obtain a high power density, spacecraft usually use hollow-cup motors with trapezoidal air-gap magnetic field waveforms. However, due to structural issues, the hollow-cup motor has the problem that the waveform of the air-gap magnetic field is inconsistent with the ideal trapezoidal waveform, which causes torque ripples. In order to reduce torque ripples, the existing method only changes the structure of PMs; the changed PMs are difficult to magnetize and manufacture, which causes the air-gap magnetic field waveform to be unsuitable as the ideal waveform. This paper proposes a novel design of an inner rotor of a hollow-cup motor with an eccentric inner rotor based on the characteristics that the hollow-cup motor has inner and outer rotors and the two rotors rotate synchronously during operation. First, the influencing factors of the air-gap magnetic field are analyzed and the mathematical model of the eccentric inner rotor is established. Then, an eccentric model is established by finite element analysis, which proves that the eccentricity of the inner rotor can make the air-gap magnetic field waveform closer to the ideal trapezoid. Finally, a prototype based on the optimal eccentricity value is developed, verifying the effectiveness of the novel design of the inner rotor.
Network Structure Engineering of Organosilica Membranes for Enhanced CO2 Capture Performance
Authors: Jiang Qiwei , Guo Meng
Published: April 2022

The membrane separation process for targeted CO2 capture application has attracted much attention due to the significant advantages of saving energy and reducing consumption. High-performance separation membranes are a key factor in the membrane separation system. In the present study, we conducted a detailed examination of the effect of calcination temperatures on the network structures of organosilica membranes. Bis(triethoxysilyl)acetylene (BTESA) was selected as a precursor for membrane fabrication via the sol-gel strategy. Calcination temperatures affected the silanol density and the membrane pore size, which was evidenced by the characterization of FT-IR, TG, N2 sorption, and molecular size dependent gas permeance. BTESA membrane fabricated at 500 °C showed a loose structure attributed to the decomposed acetylene bridges and featured an ultrahigh CO2 permeance around 15,531 GPU, but low CO2/N2 selectivity of 3.8. BTESA membrane calcined at 100 °C exhibited satisfactory CO2 permeance of 3434 GPU and the CO2/N2 selectivity of 22, displaying great potential for practical CO2 capture application.
Pilot Scale Application of a Ceramic Membrane Bioreactor for Treating High-Salinity Oil Production Wastewater
Authors: Sun Ronglin , Jin Yue
Published: April 2022

The offshore oil extraction process generates copious amounts of high-salinity oil-bearing wastewater; at present, treating such wastewater in an efficient and low-consumption manner is a major challenge. In this study, a flat ceramic membrane bioreactor (C−MBR) process combining aerobic microbial treatment technology and ceramic membrane filtration technology was used to treat oil-bearing wastewater. The pilot test results demonstrated the remarkable performance of the combined sequential batch reactor (SBR) and C-MBR process, wherein the chemical oxygen demand (COD) and ammonia nitrogen (NH4+−N) removal rates reached 93% and 98.9%, respectively. Microbial analysis indicated that the symbiosis between Marinobacterium, Marinobacter, and Nitrosomonas might have contributed to simultaneously removing NH4+−N and reducing COD, and the increased enrichment of Nitrosomonas significantly improved the nitrogen removal efficiency. Cleaning ceramic membranes with NaClO solution reduces membrane contamination and membrane cleaning frequency. The combined SBR and C−MBR process is an economical and feasible solution for treating high-salinity oil-bearing wastewater. Based on the pilot application study, the capital expenditure for operating the full-scale combined SBR and C−MBR process was estimated to be 251,717 USD/year, and the unit wastewater treatment cost was 0.21 USD/m3, which saved 62.5% of the energy cost compared to the conventional MBR process.
Design and Modeling of a Novel Tripteron-Inspired Triaxial Parallel Compliant Manipulator with Compact Structure
Authors: Xie Yanlin , Li Yangmin , Cheung Chifai
Published: April 2022

Compliant mechanisms are popular to the applications of micro/nanoscale manipulations. This paper proposes a novel triaxial parallel-kinematic compliant manipulator inspired by the Tripteron mechanism. Compared to most conventional triaxial compliant mechanisms, the proposed manipulator has the merits of structure compactness and being free of assembly error due to its unique configuration and the utilize of 3D printing technology. The compliance matrix modeling method is employed to determine the input stiffness of the compliant manipulator, and it is verified by finite-element analysis (FEA). Results show that the deviations between simulation works and the derived analytical models are in an acceptable range. The simulation results also reveal that the compliant manipulator can achieve a 16 μm × 16 μm × 16 μm cubic workspace. In this motion range, the observed maximum stress is much lower than the yield strength of the material. Moreover, the dynamic characteristics of the manipulator are investigated via the simulations as well.
Efficient Fmoc-Protected Amino Ester Hydrolysis Using Green Calcium(II) Iodide as a Protective Agent
Authors: Binette Renaud , Desgagné Michael , Theaud Camille , Boudreault Pierre-Luc
Published: April 2022

In order to modify amino acids, the C-terminus carboxylic acid usually needs to be protected, typically as a methyl ester. However, standard cleavage of methyl esters requires either highly basic or acidic conditions, which are not compatible with Fmoc or acid-labile protecting groups. This highlights the need for orthogonal conditions that permit selective deprotection of esters to create SPPS-ready amino acids. Herein, mild orthogonal ester hydrolysis conditions are systematically explored using calcium(II) iodide as a protective agent for the Fmoc protecting group and optimized for a broad scope of amino esters. Our optimized reaction improved on the already known trimethyltin hydroxide, as it produced better yields with greener, inexpensive chemicals and a less extensive energy expenditure.
Synthesis of (Hyper)Branched Monohydroxyl Alkoxysilane Oligomers toward Silanized Urethane Prepolymers
Authors: Kowalczyk Sebastian , Dębowski Maciej , Iuliano Anna , Brzeski Sebastian , Plichta Andrzej
Published: April 2022

The aim of this work was the synthesis of (hyper)branched oligomers based on trialkoxysilane in various conditions and further application of them in order to modify the urethane prepolymers. Hydroxyl-terminated trialkoxysilane was used as a monomer for homo-condensation. It was obtained by reaction of 3-aminopropyl trialkoxysilane (APTES) with ethylene carbonate (EC). The reaction was based on the attack of amine at the carbonyl carbon atom followed by ring opening of the carbonate to give a urethane (carbamate) product. The next step was the condensation via substitution of ethoxy groups on silicon atom with the terminal hydroxyalkyl groups present in the primary product with the evolution of ethanol. Accordingly, the impact of temperature and type of catalyst on process efficiency was investigated. A quantitative analysis of reaction progress and products of the conversion of EC together with ethanol evolution was conducted by means of gas chromatography, which allowed us to determine the formation of monomeric product and, indirectly, of oligomeric products. It was found that at room temperature after 24 h, the majority of the monomeric product was isolated, whereas at elevated temperature in the presence of Ti-based catalyst, further condensation of the monomer into branched oligomers was preferred, and, moreover, the application of vacuum intensified that process. The obtained products were structurally characterized by 1H and 29Si NMR, MALDI-ToF and Gel Permeation Chromatography. Finally, two different alkoxysilane products, monomeric and oligomeric, were applied for modification of urethane prepolymer, forming silanized one (SPUR). The influence of the silanizing agent on the mechanical and thermal properties of the moisture-cured products was shown before and after additional conditioning in water.
Binary Effects of Gynostemma Gold Nanoparticles on Obesity and Inflammation via Downregulation of PPARγ/CEPBα and TNF-α Gene Expression
Authors: Akter Reshmi , Ling Li , Rupa Esrat Jahan , KyuPark Jin , Mathiyalagan Ramya , Nahar Jinnatun , Won Lee Jong , Hyun Kim Do , Murugesan Mohanapriya , Yang Deok Chun , Kang Se Chan , Kwak Gi-Young
Published: April 2022

Nanoscience is a multidisciplinary skill with elucidated nanoscale particles and their advantages in applications to various fields. Owing to their economical synthesis, biocompatible nature, and widespread biomedical and environmental applications, the green synthesis of metal nanoparticles using medicinal plants has become a potential research area in biomedical research and functional food formulations. Gynostemma pentaphyllum (GP) has been extensively used in traditional Chinese medicine to cure several diseases, including diabetes mellitus (DM). This is the first study in which we examined the efficacy of G. pentaphyllum gold nanoparticles (GP-AuNPs) against obesity and related inflammation. GP extract was used as a capping agent to reduce Au2+ to Au0 to form stable gold nanoparticles. The nanoparticles were characterized by using UV–VIS spectroscopy, and TEM images were used to analyze morphology. In contrast, the existence of the functional group was measured using FTIR, and size and shape were examined using XRD analysis. In vitro analysis on GP-AuNPs was nontoxic to RAW 264.7 cells and 3T3-L1 cells up to a specific concentration. It significantly decreased lipid accumulation in 3T3-L1 obese and reduced NO production in Raw 264.7 macrophage cells. The significant adipogenic genes PPARγ and CEPBα and a major pro-inflammatory cytokine TNF-α expression were quantified using RT-PCR. The GP-AuNPs decreased the face of these genes remarkably, revealing the antiadipogenic and anti-inflammatory activity of our synthesized GP-AuNPs. This study represents thorough research on the antiobesity effect of Gynostemma pentaphyllum gold nanoparticles synthesized using a green approach and the efficacy instead of related inflammatory responses.
Single and Mixture Toxicity of Boron and Vanadium Nanoparticles in the Soil Annelid Enchytraeus crypticus: A Multi-Biomarker Approach
Authors: Capitão Ana , Santos Joana , Barreto Angela , Amorim Mónica J. B. , Maria Vera L.
Published: April 2022

The increased use and production of new materials has contributed to Anthropocene biodiversity decrease. Therefore, a careful and effective toxicity evaluation of these new materials is crucial. However, environmental risk assessment is facing new challenges due to the specific characteristics of nanomaterials (NMs). Most of the available ecotoxicity studies target the aquatic ecosystems and single exposures of NMs. The present study evaluated Enchytraeus crypticus survival and reproduction (28 days) and biochemical responses (14 days) when exposed to nanoparticles of vanadium (VNPs) and boron (BNPs) (single and mixture; tested concentrations: 10 and 50 mg/kg). Although at the organism level the combined exposures (VNPs + BNPs) did not induce a different toxicity from the single exposures, the biochemical analysis revealed a more complex picture. VNPs presented a higher toxicity than BNPs. VNPs (50 mg/kg), independently of the presence of BNPs (additive or independent effects), caused a decrease in survival and reproduction. However, acetylcholinesterase, glutathione S-transferase, catalase, glutathione reductase activities, and lipid peroxidation levels revealed alterations in neurotoxicity, detoxification and antioxidant responses, depending on the time and type of exposure (single or mixture). The results from this study highlight different responses of the organisms to contaminants in single versus mixture exposures, mainly at the biochemical level.
The Effect of Non-Pharmacological and Pharmacological Interventions on Measures Associated with Sarcopenia in End-Stage Kidney Disease: A Systematic Review and Meta-Analysis
Authors: March Daniel S. , Wilkinson Thomas J. , Burnell Thomas , Billany Roseanne E. , Jackson Katherine , Baker Luke A. , Thomas Amal , Robinson Katherine A. , Watson Emma L. , Graham-Brown Matthew P. M. , Jones Arwel W. , Burton James O.
Published: April 2022

This systematic review and meta-analysis provides a synthesis of the available evidence for the effects of interventions on outcome measures associated with sarcopenia in end-stage kidney disease (ESKD). Thirteen databases were searched, supplemented with internet and hand searching. Randomised controlled trials of non-pharmacological or pharmacological interventions in adults with ESKD were eligible. Trials were restricted to those which had reported measures of sarcopenia. Primary outcome measures were hand grip strength and sit-to-stand tests. Sixty-four trials were eligible (with nineteen being included in meta-analyses). Synthesised data indicated that intradialytic exercise increased hand grip strength (standardised mean difference, 0.58; 0.24 to 0.91; p = 0.0007; I2 = 40%), and sit-to-stand (STS) 60 score (mean difference, 3.74 repetitions; 2.35 to 5.14; p < 0.001; I2 = 0%). Intradialytic exercise alone, and protein supplementation alone, resulted in no statistically significant change in STS5 (−0.78 s; −1.86 to 0.30; p = 0.16; I2 = 0%), and STS30 (MD, 0.97 repetitions; −0.16 to 2.10; p = 0.09; I2 = 0%) performance, respectively. For secondary outcomes, L-carnitine and nandrolone-decanoate resulted in significant increases in muscle quantity in the dialysis population. Intradialytic exercise modifies measures of sarcopenia in the haemodialysis population; however, the majority of trials were low in quality. There is limited evidence for efficacious interventions in the peritoneal dialysis and transplant recipient populations.
PN Administration in Critically Ill Children in Different Phases of the Stress Response
Authors: Joosten Koen , Verbruggen Sascha
Published: April 2022

Nutritional support is an important part of the treatment of critical ill children and the phase of disease has to be taken into account. The metabolic stress response during acute critical illness is characterized by severe catabolism. So far, there is no evidence that the acute catabolic state can be prevented with nutritional support. The Pediatric ’Early versus Late Parenteral Nutrition’ (PEPaNIC) trial showed that withholding supplemental parenteral nutrition (PN) during the first week in critically ill children, when enteral nutrition was not sufficient, prevented infections and shortened the stay in the pediatric intensive care unit (PICU) and the hospital. A follow-up performed 2 and 4 years later showed that withholding parenteral nutrition (PN) also improved several domains of the neurocognitive outcome of the children. Current international guidelines recommend considering withholding parenteral macronutrients during the first week of pediatric critical illness, while providing micronutrients. These guidelines also recommend upper and lower levels of intake of macronutrients and micronutrients if PN is administered.
Calcifediol (25OHD) Deficiency and Its Treatment in Women’s Health and Fertility
Authors: Arnanz Ana , Garcia-Velasco Juan A. , Neyro José Luis
Published: April 2022

Currently, there is abundant scientific evidence showing that the vitamin D endocrine system (VDES) is a highly complex endocrine system with multiple actions in different regions of the body. The unequivocal presence of vitamin D receptors in different tissues related to fertility, and to specific aspects of women’s health such as pregnancy, undoubtedly implies functions of this steroid hormone in both male and female fertility and establishes relationships with different outcomes of human gestation. In order to review the role of the VDES in human fertility, we evaluated the relationships established between 25-hydroxyvitamin D (calcifediol) deficiency and in vitro fertilization, as well as aspects related to ovarian reserve and fertility, and commonly diagnosed endocrinopathies such as polycystic ovary disease. Likewise, we briefly reviewed the relationships between calcifediol deficiency and uterine fibroids, as well as the role that treatment may have in improving human fertility. Finally, the best scientific evidence available on the consequences of calcifediol deficiency during pregnancy is reviewed in relation to those aspects that have accumulated the most scientific literature to date, such as the relationship with the weight of the newborn at the time of delivery, the appearance of preeclampsia, and the risk of developing gestational diabetes and its final consequences for the pregnancy. To date, there is no definitive consensus on the necessary dose for treatment of calcifediol deficiency in the therapeutic management of infertility or during pregnancy. Large prospective clinical intervention studies are needed to clarify the benefits associated with this supplementation and the optimal dose to use in each situation. Although most intervention studies to date have been conducted with cholecalciferol, due to its much longer history of use in daily care, the use of calcifediol to alleviate 25-hydroxyvitamin D deficiency seems safe, even during pregnancy. The unequivocal presence of vitamin D receptors in very different tissues related to human fertility, both male and female, as well as in structures typical of pregnancy, allows us to investigate the crucial role that this steroid hormone has in specific aspects of women’s health, such as pregnancy and the ability to conceive. Well-designed clinical studies are needed to elucidate the necessary dose and the best form of treatment to resolve the very common calcifediol deficiency in women of reproductive age.
A Cross-Sectional Audit of Sorghum in Selected Cereal Food Products in Australian Supermarkets
Authors: Ducksbury Cecily , Stefoska-Needham Anita
Published: April 2022

Sorghum (Sorghum bicolor (L.) Moench) may play a role in mechanisms that elicit favourable health effects. In Australia, sorghum is successfully grown, but it is not widely consumed, and its presence in common food products is unknown. This study examined the utilisation of sorghum in common food products, specifically breakfast cereals and snack bars, in a cross-sectional study of five supermarkets in New South Wales, over a 7-day period in February 2020. Details relating to ingredients, food format, brand, and product name were recorded. Sorghum was present in 6.1% (23/379) of breakfast cereals in a variety of formats, such as extruded shapes, flour, and puffed grain. In 8.7% of these, sorghum was listed as the first ingredient (greatest contribution by weight). Sorghum was utilised in 2% (6/298) of snack bars mainly as puffed sorghum and was listed in the fourth or subsequent position in the ingredient lists for all. ‘Sorghum’ did not appear in the name of any products. In conclusion, this baseline study indicates that sorghum is present in a small proportion of breakfast cereals and snack bars, highlighting the opportunity for greater investment in sorghum food innovation and marketing that would encourage consumer recognition and expand the product range.
Curcumin Supplementation Ameliorates Bile Cholesterol Supersaturation in Hamsters by Modulating Gut Microbiota and Cholesterol Absorption
Authors: Hong Ting , Zou Jun , Jiang Xin , Yang Jie , Cao Zhuo , He Youming , Feng Dan
Published: April 2022

Curcumin is a polyphenol that has been shown to have prebiotic and cholesterol-lowering properties. This study aimed to investigate the impact of curcumin on bile cholesterol supersaturation and the potential mechanistic role of intestinal microbiota and cholesterol absorption. Male hamsters (n = 8) were fed a high-fat diet (HFD) supplemented with or without curcumin for 12 weeks. Results showed that curcumin significantly decreased cholesterol levels in the serum (from 5.10 to 4.10 mmol/L) and liver (from 64.60 to 47.72 nmol/mg protein) in HFD-fed hamsters and reduced the bile cholesterol saturation index (CSI) from 1.64 to 1.08 due to the beneficial modifications in the concentration of total bile acids (BAs), phospholipids and cholesterol (p < 0.05). Gut microbiota analysis via 16S rRNA sequencing revealed that curcumin modulated gut microbiota, predominantly increasing microbiota associated with BA metabolism and short-chain fatty acid production, which subsequently up-regulated the expression of hepatic cholesterol 7-alpha hydroxylase and increased the synthesis of bile acids (p < 0.05). Furthermore, curcumin significantly down-regulated the expression of intestinal Niemann–Pick C1-like protein 1(NPC1L1) in hamsters and reduced cholesterol absorption in Caco-2 cells (p < 0.05). Our results demonstrate that dietary curcumin has the potential to prevent bile cholesterol supersaturation through modulating the gut microbiota and inhibiting intestinal cholesterol absorption.
Grape Seed Proanthocyanidins Mitigate the Disturbances Caused by an Abrupt Photoperiod Change in Healthy and Obese Rats
Authors: Soliz-Rueda Jorge R. , López-Fernández-Sobrino Raúl , Bravo Francisca Isabel , Aragonès Gerard , Suarez Manuel , Muguerza Begoña
Published: April 2022

Variations in the light/dark cycle and obesogenic diets trigger physiological and behavioral disorders. Proanthocyanidins, in addition to their healthy properties, have recently demonstrated a modulating effect on biological rhythms. Therefore, the aim of this study was to evaluate the administration of a grape seed proanthocyanidin-rich extract (GSPE) to mitigate the disruption caused by a sudden photoperiod change in healthy and cafeteria (CAF)-diet obese rats. For this, 48 photoperiod-sensitive Fischer 344 rats were fed standard or CAF diets for 6 weeks under a standard (12 h light/day, L12) conditions. Then, rats were switched to a long (18 h light/day, L18) or short (6 h light/day, L6) photoperiod and administered vehicle or GSPE (25 mg/kg) for 1 week. Body weight (BW) and food intake (FI) were recorded weekly. Animal activity and serum hormone concentrations were studied before and after the photoperiod change. Hormone levels were measured both at 3 h (ZT3) and 15 h (ZT15) after the onset of light. Results showed the impact of the CAF diet and photoperiod on the BW, FI, activity, and hormonal status of the animals. GSPE administration resulted in an attenuation of the changes produced by the photoperiod disruption. Specifically, GSPE in L6 CAF-fed rats reduced serum corticosterone concentration, restoring its circadian rhythm, increased the T3-to-T4 ratio, and increased light phase activity, while under L18, it decreased BW and testosterone concentration and increased the animal activity. These results suggest that GSPE may contribute to the adaptation to the new photoperiods. However, further studies are needed to elucidate the metabolic pathways and processes involved in these events.
Synthesis, Biological Activity, and Molecular Modelling Studies of Naphthoquinone Derivatives as Promising Anticancer Candidates Targeting COX-2
Authors: Kavaliauskas Povilas , Opazo Felipe Stambuk , Acevedo Waldo , Petraitiene Ruta , Grybaitė Birutė , Anusevičius Kazimieras , Mickevičius Vytautas , Belyakov Sergey , Petraitis Vidmantas
Published: April 2022

Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-associated mortalities worldwide. Therefore, it is crucial to develop a novel therapeutic option targeting localized and metastatic NSCLC. In this paper, we describe the synthesis and biological activity characterization of naphthoquinone derivatives bearing selective anticancer activity to NSCLC via a COX-2 mediated pathway. The biological evaluation of compounds 9–16 showed promising structure-dependent anticancer activity on A549 cells in 2D and 3D models. Compounds were able to significantly (p < 0.05) reduce the A549 viability after 24 h of treatment in comparison to treated control. Compounds 9 and 16 bearing phenylamino and 4-hydroxyphenylamino substituents demonstrated the most promising anticancer activity and were able to induce mitochondrial damage and ROS formation. Furthermore, most promising compounds showed significantly lower cytotoxicity to non-cancerous Vero cells. The in silico ADMET properties revealed promising drug-like properties of compounds 9 and 16. Both compounds demonstrated favorable predicted GI absorption values, while only 16 was predicted to be permeable through the blood–brain barrier. Molecular modeling studies identified that compound 16 is able to interact with COX-2 in arachidonic acid site. Further studies are needed to better understand the safety and in vivo efficacy of compounds 9 and 16.
Genetic Variant ABCC1 rs45511401 Is Associated with Increased Response to Statins in Patients with Familial Hypercholesterolemia
Authors: Dagli-Hernandez Carolina , Borges Jéssica Bassani , Marçal Elisangela da Silva Rodrigues , de Freitas Renata Caroline Costa , Mori Augusto Akira , Gonçalves Rodrigo Marques , Faludi Andre Arpad , de Oliveira Victor Fernandes , Ferreira Glaucio Monteiro , Bastos Gisele Medeiros , Zhou Yitian , Lauschke Volker M. , Cerda Alvaro , Hirata Mario Hiroyuki , Hirata Rosario Dominguez Crespo
Published: April 2022

Statins are the first-line treatment for familial hypercholesterolemia (FH), but response is highly variable due to genetic and nongenetic factors. Here, we explored the association between response and genetic variability in 114 Brazilian adult FH patients. Specifically, a panel of 84 genes was analyzed by exon-targeted gene sequencing (ETGS), and the functional impact of variants in pharmacokinetic (PK) genes was assessed using an array of functionality prediction methods. Low-density lipoprotein cholesterol (LDL-c) response to statins (reduction ≥ 50%) and statin-related adverse event (SRAE) risk were assessed in carriers of deleterious variants in PK-related genes using multivariate linear regression analyses. Fifty-eight (50.8%) FH patients responded to statins, and 24 (21.0%) had SRAE. Results of the multivariate regression analysis revealed that ABCC1 rs45511401 significantly increased LDL-c reduction after statin treatment (p < 0.05). In silico analysis of the amino-acid change using molecular docking showed that ABCC1 rs45511401 possibly impairs statin efflux. Deleterious variants in PK genes were not associated with an increased risk of SRAE. In conclusion, the deleterious variant ABCC1 rs45511401 enhanced LDL-c response in Brazilian FH patients. As such, this variant might be a promising candidate for the individualization of statin therapy.
DNA Copy Number Aberrations and Expression of ABC Transporter Genes in Breast Tumour: Correlation with the Effect of Neoadjuvant Chemotherapy and Prognosis of the Disease
Authors: Tsyganov Matvey M. , Ibragimova Marina K. , Gaptulbarova Kseniya A. , Tsydenova Irina A. , Dolgasheva Daria S. , Garbukov Evgeniy Y. , Frolova Anastasia A. , Slonimskaya Elena M. , Litvyakov Nikolai V.
Published: April 2022

One of the important reasons for the ineffectiveness of chemotherapy in breast cancer (BC) is considered to be the formation of a multidrug resistance phenotype in tumour cells, which is caused by the expression of energy-dependent ABC transporters. The aim of this work was to assess chromosomal aberrations and the level of transcripts of all 49 known ABC transporter genes in breast tumours. Materials and Methods. The study included 129 patients with breast cancer. A microarray study of all tumour samples was carried out on microchips. Results. This study established that the presence of a deletion in genes ABCB1, ABCB4, ABCB8, ABCC7, ABCC11, ABCC12, ABCF2, and ABCG4 is associated with an objective response to treatment (p ≤ 0.05). A decrease in the expression of genes was associated with a good response to chemotherapy, whereas an increase in expression caused the progression and stabilization of the tumour. Analysis of metastatic-free survival rates showed that the presence of ABCB1/4 and ABCC1/6 deletions was associated with 100% survival (log-rank test p = 0.01 and p = 0.03). Conclusions. The study showed that the aberrant state of ABC transporter genes, as well as a decrease in the expression of these genes, is a predictor of the effectiveness of therapeutic treatment and a potential prognostic marker of metastatic survival.