bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Immunofluorescence Technique to Perform a Quantitative Analysis of Masseter Muscle Fibers in Unilateral Posterior Crossbite: A Pilot Study
Authors: Vermiglio Giovanna , Piancino Mariagrazia , Runci Anastasi Michele , Picciolo Giacomo , Centofanti Antonio , Santoro Giuseppe , Malandrino Mariachiara , Cutroneo Giuseppina , Anastasi Giuseppe
Published: June 2021

Unilateral posterior crossbite is a type of malocclusion that involves morpho-functional characteristics of masticatory muscle, such as the masseter: electrophysiological data have shown that the affected side works less than the contralateral muscle, which shows a normal or increased activity, probably in order to compensate for the affected side. The aim of present work was to measure the diameter and the cross-sectional area of ipsilateral and contralateral muscle fibers to verify if hypertrophy and/or hypotrophy take place in this malocclusion. We used immunofluorescence pictures to measure, using ImageJ software, the diameter and the cross-sectional area of fibers from control and crossbite groups; after that, the data were processed to perform statistical analyses. Results show that the fiber diameters of contralateral muscle are larger than the diameters of ipsilateral and control fibers, and that this difference is statistically significant. No statistically significant difference was found between the fiber diameters of the ipsilateral and control muscles. All these data suggest that, during unilateral posterior crossbite, morphological changes take place in the contralateral masseter muscle, which undergoes hypertrophy, probably to compensate for the low activity of the affected muscle.
Climate Change Impacts on Temperature and Chill Unit Trends for Apple (Malus domestica) Production in Ceres, South Africa
Authors: Tharaga Phumudzo Charles , Steyn Abraham Stephanus , Coetzer Gesine Maria
Published: June 2021

Climate is an essential part of crop production, determining the suitability of a given region for deciduous fruit products such as apples (Malus domestica). It influences the yield and quality of fruits. There is strong evidence of global and regional-scale climate change since the advent of the industrial era. In South Africa, mean surface temperatures have revealed a warming trend over the last century. This study aimed to assess the impact of climate change on temperature and chill unit trends for apple production in Ceres, South Africa. The daily positive Utah chill units (DPCU) model was used as frequent high temperatures can lead to a high negation volume. Historically observed (1981–2010) and future projected (2011–2100) temperatures were obtained from the South African Weather Service (SAWS) and three ensemble members of the Cubic-Conformal Atmospheric Model (CCAM), respectively. The latter employed the RCP8.5 pathway. Linear trends were calculated for temperature and accumulated PCUs for the historical base period. The probability of accumulating specific threshold PCU values for both historical and future periods was assessed from cumulative distribution functions (CDFs). The historical change in minimum temperatures showed no significant trend. Ceres revealed a warming trend in maximum temperatures over the historical period. By the 2080s, the probability of not exceeding a threshold of 1600 PCUs was exceptionally high for all ensemble members. Future projections showed a decline in the accumulated PCUs of 2–5% by the 2020s, 7–17% by the 2050s, and 20–34% towards the end of the 20th century. Based on these results, it is clear that winter chill units are negatively influenced by climate change. The loss in yield and fruit quality of apples due to climate change can negatively impact the export market, leading to significant economic losses for apple production in the Ceres area.
Multi-Decadal Variability and Future Changes in Precipitation over Southern Africa
Authors: Lim Kam Sian Kenny Thiam Choy , Wang Jianhong , Ayugi Brian Odhiambo , Nooni Isaac Kwesi , Ongoma Victor
Published: June 2021

The future planning and management of water resources ought to be based on climate change projections at relevant temporal and spatial scales. This work uses the new regional demarcation for Southern Africa (SA) to investigate the spatio-temporal precipitation variability and trends of centennial-scale observation and modeled data, based on datasets from the sixth phase of the Coupled Model Intercomparison Project (CMIP6). The study employs several statistical methods to rank the models according to their precipitation simulation ability. The Theil–Sen slope estimator is used to assess precipitation trends, with a Student’s t-test for the significance test. The comparison of observation and model historical data enables identification of the best-performing global climate models (GCMs), which are then employed in the projection analysis under two Shared Socioeconomic Pathways (SSPs): SSP2-4.5 and SSP5-8.5. The GCMs adequately capture the annual precipitation variation but with a general overestimation, especially over high-elevation areas. Most of the models fail to capture precipitation over the Lesotho-Eswatini area. The three best-performing GCMs over SA are FGOALS-g3, MPI-ESM1-2-HR and NorESM2-LM. The sub-regions demonstrate that precipitation trends cannot be generalized and that localized studies can provide more accurate findings. Overall, precipitation in the wet and dry seasons shows an initial increase during the near future over western and eastern SA, followed by a reduction in precipitation during the mid- and far future under both projection scenarios. Madagascar is expected to experience a decrease in precipitation amount throughout the twenty-first century.
Biomarkers for Diagnosis, Prognosis and Response to Immunotherapy in Melanoma
Authors: Puglisi Rossella , Bellenghi Maria , Pontecorvi Giada , Pallante Giulia , Carè Alessandra , Mattia Gianfranco
Published: June 2021

Cutaneous Melanoma classification is constantly looking for specific and sensitive biomarkers capable of having a positive effect on diagnosis, prognosis and risk assessment, eventually affecting clinical outcome. Classical morphological, immunohistochemical and the well-known BRAF and NRAS genetic biomarkers do not allow the correct categorization of patients, being melanoma conditioned by high genetic heterogeneity. At the same time, classic prognostic methods are unsatisfactory. Therefore, new advances in omics and high-throughput analytical techniques have enabled the identification of numerous possible biomarkers, but their potentiality needs to be validated and standardized in prospective studies. Melanoma is considered an immunogenic tumor, being the first form of cancer to take advantage of the clinical use of the immune-checkpoint blockers. However, as immunotherapy is effective only in a limited number of patients, biomarkers associated with different responses are essential to select the more promising therapeutic approach and maximize clinical benefits. In this review, we summarize the most utilized biomarkers for Cutaneous Melanoma diagnosis, focusing on new prognostic and predictive biomarkers mainly associated with immunotherapy.
Targeting HIF-1α Regulatory Pathways as a Strategy to Hamper Tumor-Microenvironment Interactions in CLL
Authors: Vitale Candida , Griggio Valentina , Riganti Chiara , Todaro Maria , Kopecka Joanna , Jones Rebecca , Salvetti Chiara , Boccellato Elia , Perutelli Francesca , Voena Claudia , Godio Laura , Boccadoro Mario , Coscia Marta
Published: June 2021

The hypoxia-inducible factor 1 (HIF-1) and the CXCL12/CXCR4 axis regulate the interaction of chronic lymphocytic leukemia cells and the tumor microenvironment. However, the interconnections occurring between HIF-1 and the CXCL12/CXCR4 axis are not fully elucidated. Here, we demonstrate that the CXCL12/CXCR4 axis plays a pivotal role in the positive regulation of the α subunit of HIF-1 (HIF-1α) that occurs in CLL cells co-cultured with stromal cells (SC). Inhibitors acting at different levels on CXCR4 downstream signalling counteract the SC-induced HIF-1α upregulation in CLL cells, also hindering the SC-mediated pro-survival effect. HIF-1α inhibition also exerts off-tumor effects on the SC component, inducing the downregulation of target genes, including CXCL12. Consistently, our data show that pretreatment of leukemic cells and/or SC with idelalisib effectively abrogates the SC-mediated survival support. A combined on-tumor and off-tumor inhibition of HIF-1α was also observed in idelalisib-treated patients, who showed, along with a downregulation of HIF-1α target genes in leukemic cells, a significant decrease in CXCL12 serum concentration and changes in the bone marrow microenvironment. Our data demonstrate that the targeting of HIF-1α or its regulatory pathways acts at the tumor- and SC-level, and may be an appealing strategy to overcome the microenvironment-mediated protection of CLL cells.
A Comparison of Hypofractionated and Twice-Daily Thoracic Irradiation in Limited-Stage Small-Cell Lung Cancer: An Overlap-Weighted Analysis
Authors: Yan Michael , Sigurdson Samantha , Greifer Noah , Kennedy Thomas A. C. , Toh Tzen S. , Lindsay Patricia E. , Weiss Jessica , Hueniken Katrina , Yeung Christy , Sugumar Vijithan , Sun Alexander , Bezjak Andrea , Cho B. C. John , Raman Srinivas , Hope Andrew J. , Giuliani Meredith E. , Stuart Elizabeth A. , Owen Timothy , Ashworth Allison , Robinson Andrew , de Moraes Fabio Ynoe , Liu Geoffrey , Lok Benjamin H.
Published: June 2021

Despite evidence for the superiority of twice-daily (BID) radiotherapy schedules, their utilization in practice remains logistically challenging. Hypofractionation (HFRT) is a commonly implemented alternative. We aim to compare the outcomes and toxicities in limited-stage small-cell lung cancer (LS-SCLC) patients treated with hypofractionated versus BID schedules. A bi-institutional retrospective cohort review was conducted of LS-SCLC patients treated with BID (45 Gy/30 fractions) or HFRT (40 Gy/15 fractions) schedules from 2007 to 2019. Overlap weighting using propensity scores was performed to balance observed covariates between the two radiotherapy schedule groups. Effect estimates of radiotherapy schedule on overall survival (OS), locoregional recurrence (LRR) risk, thoracic response, any ≥grade 3 (including lung, and esophageal) toxicity were determined using multivariable regression modelling. A total of 173 patients were included in the overlap-weighted analysis, with 110 patients having received BID treatment, and 63 treated by HFRT. The median follow-up was 20.4 months. Multivariable regression modelling did not reveal any significant differences in OS (hazard ratio [HR] 1.67, p = 0.38), LRR risk (HR 1.48, p = 0.38), thoracic response (odds ratio [OR] 0.23, p = 0.21), any ≥grade 3+ toxicity (OR 1.67, p = 0.33), ≥grade 3 pneumonitis (OR 1.14, p = 0.84), or ≥grade 3 esophagitis (OR 1.41, p = 0.62). HFRT, in comparison to BID radiotherapy schedules, does not appear to result in significantly different survival, locoregional control, or toxicity outcomes.
Rintatolimod Induces Antiviral Activities in Human Pancreatic Cancer Cells: Opening for an Anti-COVID-19 Opportunity in Cancer Patients?
Authors: Mustafa Dana A. M. , Saida Lawlaw , Latifi Diba , Wismans Leonoor V. , de Koning Willem , Zeneyedpour Lona , Luider Theo M. , van den Hoogen Bernadette , van Eijck Casper H. J.
Published: June 2021

Severe acute respiratory virus-2 (SARS-CoV-2) has spread globally leading to a devastating loss of life. Large registry studies have begun to shed light on the epidemiological and clinical vulnerabilities of cancer patients who succumb to or endure poor outcomes of SARS-CoV-2. Specific treatment for COVID-19 infections in cancer patients is lacking while the demand for treatment is increasing. Therefore, we explored the effect of Rintatolimod (Ampligen®) (AIM ImmunoTech, Ocala, FL, USA), a Toll-like receptor 3 (TLR3) agonist, to treat uninfected human pancreatic cancer cells (HPACs). The direct effect of Rintatolimod was measured by targeted gene expression profiling and by proteomics measurements. Our results show that Rintatolimod induces an antiviral effect in HPACs by inducing RNase-L-dependent and independent pathways of the innate immune system. Treatment with Rintatolimod activated the interferon signaling pathway, leading to the overexpression of several cytokines and chemokines in epithelial cells. Furthermore, Rintatolimod treatment increased the expression of angiogenesis-related genes without promoting fibrosis, which is the main cause of death in patients with COVID-19. We conclude that Rintatolimod could be considered an early additional treatment option for cancer patients who are infected with SARS-CoV-2 to prevent the complicated severity of the disease.
Antibody–Drug Conjugates for the Treatment of Breast Cancer
Authors: Corti Chiara , Giugliano Federica , Nicolò Eleonora , Ascione Liliana , Curigliano Giuseppe
Published: June 2021

Metastatic breast cancer (BC) is currently an incurable disease. Besides endocrine therapy and targeted agents, chemotherapy is often used in the treatment of this disease. However, lack of tumor specificity and toxicity associated with dose exposure limit the manageability of cytotoxic agents. Antibody–drug conjugates (ADCs) are a relatively new class of anticancer drugs. By merging the selectivity of monoclonal antibodies with the cytotoxic properties of chemotherapy, they improve the therapeutic index of antineoplastic agents. Three core components characterize ADCs: the antibody, directed to a target antigen; the payload, typically a cytotoxic agent; a linker, connecting the antibody to the payload. The most studied target antigen is HER2 with some agents, such as trastuzumab deruxtecan, showing activity not only in HER2-positive, but also in HER2-low BC patients, possibly due to a bystander effect. This property to provide a cytotoxic impact also against off-target cancer cells may overcome the intratumoral heterogeneity of some target antigens. Other cancer-associated antigens represent a strategy for the development of ADCs against triple-negative BC, as shown by the recent approval of sacituzumab govitecan. In this review, we discuss the current landscape of ADC development for the treatment of BC, as well as the possible limitations of this treatment.
Endocrine Disrupting Chemicals, Hormone Receptors, and Acne Vulgaris: A Connecting Hypothesis
Authors: Rao Akshatha , Douglas Sotonye C. , Hall Julianne M.
Published: June 2021

The relationship between endocrine disrupting chemicals (EDCs) and the pathogenesis of acne vulgaris has yet to be explored in the literature. Acne vulgaris is a chronic inflammatory skin disease of the pilosebaceous unit. The pathogenesis of acne involves several hormonal pathways, including androgens, insulin-like growth factor 1(IGF-1), estrogens, and corticosteroids. EDCs influence these pathways primarily through two mechanisms: altering endogenous hormone levels and interfering with hormone receptor function. This review article describes the mechanistic links between EDCs and the development of acne lesions. Highlighted is the contributory role of androgen receptor ligands, such as bisphenol A (BPA) and mono-2-ethylhexyl Phthalate (MEHP), via upregulation of lipogenic genes and resultant exacerbation of cholesterol synthesis. Additionally discussed is the protective role of phytoestrogen EDCs in counteracting androgen-induced sebocyte maturation through attenuation of PPARy transcriptional activity (i.e., resveratrol) and restoration of estrogen-regulated TGF-B expression in skin cells (i.e., genistein). Examination of the relationship between EDCs and acne vulgaris may inform adjunctive avenues of treatment such as limiting environmental exposures, and increasing low-glycemic, plant-rich foods in the diet. With a better understanding of the cumulative role that EDCs play in acne, clinicians can be better equipped to treat and ultimately improve the lives of their patients.
Effect of Thermal Barrier Coating on the Performance and Emissions of Diesel Engine Operated with Conventional Diesel and Palm Oil Biodiesel
Authors: Ramasamy Navin , Kalam Mohammad Abul , Varman Mahendra , Teoh Yew Heng
Published: June 2021

In this study, the performance and emission of a thermal barrier coating (TBC) engine which applied palm oil biodiesel and diesel as a fuel were evaluated. TBC was prepared by using a series of mixture consisting different blend ratio of yttria stabilized zirconia (Y2O3·ZrO2) and aluminum oxide-silicon oxide (Al2O3·SiO2) via plasma spray coating technique. The experimental results showed that mixture of TBC with 60% Y2O3·ZrO2 + 40% Al2O3·SiO2 had an excellent nitrogen oxide (NO), carbon monoxide (CO), carbon dioxide (CO2), and unburned hydrocarbon (HC) reductions compared to other blend-coated pistons. The finding also indicated that coating mixture 50% Y2O3·ZrO2 + 50% Al2O3·SiO2 had the highest brake thermal efficiency (BTE) and lowest of brake specific fuel consumption (BSFC) compared to all mixture coating. Reductions of HC and CO emissions were also recorded for 60% Y2O3·ZrO2 + 40% Al2O3·SiO2 and 50% Y2O3·ZrO2 + 50% Al2O3·SiO2 coatings. These encouraging findings had further proven the significance of TBC in enhancing the engine performance and emission reductions operated with different types of fuel.
A High-Energy, Narrow-Pulse-Width, Long-Wave Infrared Laser Based on ZGP Crystal
Authors: Qian Chuanpeng , Yu Ting , Liu Jing , Jiang Yuyao , Wang Sijie , Shi Xiangchun , Ye Xisheng , Chen Weibiao
Published: June 2021

In this paper, we present a high-energy, narrow pulse-width, long-wave infrared laser based on a ZnGeP2 (ZGP) optical parametric oscillator (OPO). The pump source is a 2.1 μm three -stage Ho:YAG master oscillator power-amplifier (MOPA). At a repetition frequency of 1 kHz, the Ho:YAG MOPA system outputs the maximal average power of 52.1 W, which corresponds to the shortest pulse width of 14.40 ns. By using the Ho:YAG MOPA system as the pump source, the maximal average output powers of 3.15 W at 8.2 μm and 11.4 W at 2.8 μm were achieved in a ZGP OPO. The peak wavelength and linewidth (FWHM) of the long-wave infrared laser were 8156 nm and 270 nm, respectively. At the maximal output level, the pulse width and beam quality factor M2 were measured to be 8.10 ns and 6.2, respectively.
Effect of Full Temperature Field Environment on Bonding Strength of Aluminum Alloy
Authors: Liu Haichao , Fan Yisa , Peng Han
Published: June 2021

In this paper, the influence of temperature on the bonding strength of aluminum alloy joints under the full temperature field is studied. Based on the service temperature range of vehicle bonding structures, the failure strength of aluminum alloy joints at different temperature points, namely −40 °C, −20 °C, 0 °C, 25 °C (RT), 40 °C, 60 °C and 80 °C, is tested. The results showed that compared with the failure strength of the adhesive at −40 °C, it decreased by 47.69% and 68.15% at RT and 80°C, respectively; the Young’s modulus of the adhesive decreased by 57.63% and 75.42% at RT and 80°C, respectively; with the increase of temperature, the young’s modulus, tensile strength and failure strain of the adhesive decreased. In addition, the failure strength of aluminum alloy joints varied with temperature. To be specific, the stiffness of joints decreased gradually from 25 °C to 80 °C and increased gradually from −40 °C. Based on the failure strength data of bonded joints at different temperature points, the secondary stress failure criteria of bonded joints at different temperatures were obtained. Then, the surface function of failure criteria under the full temperature field was established to provide reference for failure prediction of bonded structures under different temperatures and stresses.
Canadian Resources, Programs, and Models of Care to Support Cancer Survivors’ Transition beyond Treatment: A Scoping Review
Authors: Romkey-Sinasac Claudia , Saunders Stephanie , Galica Jacqueline
Published: June 2021

(1) Background: One in two Canadians will be diagnosed with cancer in their lifetime, but as a result of the progress in diagnosis and treatment, more individuals are surviving cancer than ever before. However, the impact of cancer does not end with treatment. The objectives of this review are to (1) provide a broad overview of the supportive care interventions and models of care that have been researched to support Canadian post-treatment cancer survivors; and (2) analyze how these supportive care interventions and/or care models align with the practice recommendations put forth by Cancer Care Ontario (CCO) and the Canadian Association of Psychosocial Oncology/Canadian Partnership Against Cancer (CAPO/CPAC). (2) Methods: An electronic search was completed in MEDLINE, Embase, PsycINFO, and CINAHL in January 2021. Included studies described supportive care interventions or models of care utilized by adult Canadian cancer survivors. (3) Results: Forty-two articles were included. Survivors utilized a multitude of supportive care interventions, with peer support and physical activity programs being most frequently cited. Four models of follow-up care were identified: primary care, oncology care, shared-care, and transition clinics. The supportive care interventions and models of care variably aligned with the recommendations set by CCO and CAPO/CPAC. The most commonly followed recommendation was the promotion of self-management and quality resources for patients. (4) Conclusions: Results indicate an inconsistency in access to supportive care interventions and the delivery of survivorship care for cancer survivors across Canada. Current efforts are being made to implement the recommendations by CCO and CAPO/CPAC; however, provision of these guidelines remains varied.
Is It Possible to Make Money on Investing in Companies Manufacturing Solar Components? A Panel Data Approach
Authors: Tomczak Sebastian Klaudiusz , Skowrońska-Szmer Anna , Szczygielski Jan Jakub
Published: June 2021

In the interests of the environment, many countries set limits on the use of non-renewable energy sources and promote renewable energy sources through policy and legislation. Consequently, the demand for components for renewable energy systems exhibits an upward trend. For this reason, managers, investors, and banks are interested in knowing whether investing in a business associated with the semiconductor and related device manufacturing sector, especially the photovoltaic (PV) systems manufacturers, is worthy of a penny. Using a sample for the period of 2015–2018, we apply a new approach to panel data, extending existing research using Classification Trees with the k-Nearest Neighbor and Altman model. Our aim is to analyze the financial conditions of enterprises to identify key indicators that distinguish companies producing PV system components (labeled “green, G”) from companies that do not manufacture PV components (“red, R”). Our results show that green companies can be distinguished from red companies at classification accuracies of 86% and 90% for CRT and CHAID algorithms in Classification Trees method and 93% for k-Nearest Neighbor method, respectively. Based on the Altman model and the analysis of crucial ratios, we also find that green businesses are characterized by lower financial performance although future ratio values may equal or exceed the values for the red companies if current upward trends are sustained. Therefore, investing in green companies presents a viable alternative.
On the Socio-Cultural Determinants of Polish Entrepreneurs’ Attitudes towards the Development of Renewable Energy: Business, Climate Skepticism Ideology and Climate Change
Authors: Żuk Piotr , Żuk Paweł
Published: June 2021

This article analyses the business community in Poland and their views on climate change and the objectives of the European Green Deal (EGD). The authors focus on the analysis of the attitude of entrepreneurs towards the cultural and ideological stereotypes promoted by climate denialists. This article shows that the ideological factor may play a certain role in shaping the attitudes of entrepreneurs towards energy transition. The research was carried out on a sample of 400 entrepreneurs. The research results indicate that the ideological factor is of particular importance among entrepreneurs who are concerned about the costs of energy transition and the expenditure related to the implementation of the EGD for economic reasons. According to the authors, the stereotypes promoted by climate sceptics may find fertile ground, particularly when accompanied by fears of additional financial costs. Attitudes towards the goals of energy transition among entrepreneurs usually differ depending on the size of the company: representatives of smaller companies, who have greater concerns about their economic position, are more likely to present the views of climate sceptics. Acceptance for the theory of climate sceptics may also be influenced by the industry sector: those who may lose out on energy transition, such as representatives of the mining industry, may share the myths of climate denialists.
Clinical Work and Life of Mid-Career Male Nurses: A Qualitative Study
Authors: Shin Soo-Yong , Lim Eun-Ju
Published: June 2021

In Korea, about 3000 qualified male nurses enter the clinical nursing field annually; however, they face challenges in long-term job retention in general hospitals. Therefore, this study characterized the work and life experiences of mid-career male nurses engaged in clinical nursing care. Participants were nine registered nurses with a minimum of five years’ work experience in a general hospital in Korea. Data were collected through face-to-face, in-depth, semi-structured interviews. A phenomenological qualitative design was used with Colaizzi’s data analysis method. Three categories, eight theme clusters, and seventeen themes were revealed. The three categories were “limitations and adaptation to work performance”, “interpersonal difficulties and coping”, and “facing reality and preparing for the future”. Findings suggested that mid-career male nurses were considering transitioning to other job roles. These findings help clarify the clinical work experience of mid-career male nurses and their difficulties with job retention. The results provide basic data that may inform the design of policies to practically support male nurses in preserving their careers.
Pharmacy Technicians, Stigma, and Compassion Fatigue: Front-Line Perspectives of Pharmacy and the US Opioid Epidemic
Authors: Cernasev Alina , Desselle Shane , Hohmeier Kenneth C. , Canedo Joanne , Tran Britney , Wheeler James
Published: June 2021

The opioid epidemic in the United States has led to a quadrupling of opioid overdoses since the 1990s. Stigmas exist among healthcare professionals, and it is essential to educate the next generation of pharmacy technicians regarding opioid use disorder. The main objective of this study was to characterize the phenomenon of stigma through the pharmacy technician lens when taking care of patients who are using opioid medications. Grounded in Van Manen’s phenomenological approach and the Link and Phelan stigmatization model, a qualitative study was conducted from February to June 2020 to understand pharmacy technicians’ perceptions and attitudes towards patients using opioid medications. Focus groups (n = 46) with pharmacy technicians were conducted in-person and online over five months in 2020. Thematic analysis identified three themes that characterize the stigma and the relationship between pharmacy technicians and patients taking opioid medications: (1) pharmacy technician perspectives on stigma and patients with addictive opioid-use behavior; (2) current approaches of pharmacy technicians towards patients with addictive opioid-use behavior; (3) future approaches of pharmacy technicians towards patients with addictive opioid-use behavior. The findings highlight an “ever-present” negative connotation associated with the stigma that is formed from patient interaction. It is necessary to develop proper resources and educational materials to manage the stigma that exists in pharmacies throughout the nation. These resources will facilitate how to address and prevent the stigma among pharmacy technicians in the U.S.
Polydisperse Aerosol Transport and Deposition in Upper Airways of Age-Specific Lung
Authors: Islam Mohammad S. , Larpruenrudee Puchanee , Hossain Sheikh I. , Rahimi-Gorji Mohammad , Gu Yuantong , Saha Suvash C. , Paul Gunther
Published: June 2021

A comprehensive understanding of airflow characteristics and particle transport in the human lung can be useful in modelling to inform clinical diagnosis, treatment, and management, including prescription medication and risk assessment for rehabilitation. One of the difficulties in clinical treatment of lung disorders lies in the patients’ variable physical lung characteristics caused by age, amongst other factors, such as different lung sizes. A precise understanding of the comparison between different age groups with various flow rates is missing in the literature, and this study aims to analyse the airflow and aerosol transport within the age-specific lung. ANSYS Fluent solver and the large-eddy simulation (LES) model were employed for the numerical simulation. The numerical model was validated with the available literature and the computational results showed airway size-reduction significantly affected airflow and particle transport in the upper airways. This study reports higher deposition at the mouth-throat region for larger diameter particles. The overall deposition efficiency (DE) increased with airway size reduction and flow rate. Lung aging effected the pressure distribution and a higher pressure drop was reported for the aged lung as compared to the younger lung. These findings could inform medical management through individualised simulation of drug-aerosol delivery processes for the patient-specific lung.
Multi-Component Intervention Program on Habitual Physical Activity Parameters and Cognitive Function in Patients with Mild Cognitive Impairment: A Randomized Controlled Trial
Authors: Jeong Min-Ki , Park Kyung-Won , Ryu Je-Kwang , Kim Gwon-Min , Jung Hyun-Hun , Park Hyuntae
Published: June 2021

Age-related dementia refers to a state in which someone experiences multiple cognitive function impairment due to degenerative brain disease, and which causes difficulties in their daily life or social life. Dementia is the most common and serious obstacle in later life. Early intervention in the case of patients who are in the mild cognitive impairment (MCI) stage among the high-risk group can maintain and improve their cognitive function. The purpose of the current trial is aimed at investigating the association between a multi-component (exercise with cognitive) intervention program and habitual physical activity parameters on cognitive functions in MCI patients. Neuropsychological cognitive and depression assessments were performed by neuropsychologists according to normalized methods, including the Korean mini-mental State examination (K-MMSE) and modified Alzheimer’s disease assessment scale-cognitive subscale (ADAS-Cog) and cognitive assessment tool (attention, processing speed), and the Korean version of the geriatric depression scale (SGDS-K), both at baseline and at a 12 weeks follow-up. The 12-week multi-component intervention improved cognitive function and habitual physical activity parameters in patients with MCI relative to controls. A multi-component intervention program for patients with MCI is considered to be an effective method of dementia prevention by improving global (ADAS-Cog) and frontal (trail-making test, digit symbol substitution test) cognition and habitual physical activity parameters such as moderate to vigorous physical activity and step count. In addition, it is important to encourage habitual physical activities to ensure that exercise intervention strategies are carried out at the duration and intensity required for improving physical and cognitive wellbeing and obtaining health benefits.
The Childhood Cancer Centre Is Coming Home: Experiences of Hospital-Based Home Care
Authors: de Flon Malin , Glaffey Gisela , Jarl Linda , Sellbrant Kristin , Nilsson Stefan
Published: June 2021

Today, there is a shift towards care being given closer to the patient, with more children receiving care in their homes. Care at home has proven to be a viable alternative to hospital care, as shown by a project for hospital-based home care conducted in West Sweden. The aim of this study was to describe how children with cancer and parents experienced receiving care at home. After purposive sampling, six children with cancer aged 6–16 and eight parents participated. Semistructured interviews were performed, and the data were analysed using qualitative content analysis. Four main categories emerged: save time and energy in the family; maintain everyday life; feel trust in the healthcare professionals; mixed feelings about getting treatment at home. This hospital-based home care project created good conditions for both children with cancer and their parents to feel secure. In addition, home care can be very child-centric, whereby the caregivers involve the children by taking their thoughts and utterances into account.
Decreased Methane Emissions Associated with Methanogenic and Methanotrophic Communities in a Pig Manure Windrow Composting System under Calcium Superphosphate Amendment
Authors: Zhang Yihe , Huang Mengyuan , Zheng Fengwei , Guo Shumin , Song Xiuchao , Liu Shuwei , Li Shuqing , Zou Jianwen
Published: June 2021

With the rapid growth of livestock breeding, manure composting has evolved to be an important source of atmospheric methane (CH4) which accelerates global warming. Calcium superphosphate (CaSSP), as a commonly used fertilizer, was proposed to be effective in reducing CH4 emissions from manure composting, but the intrinsic biological mechanism remains unknown. Methanogens and methanotrophs both play a key role in mediating CH4 fluxes, therefore we hypothesized that the CaSSP-mediated reduction in CH4 emissions was attributed to the shift of methanogens and methanotrophs, which was regulated by physicochemical parameter changes. To test this hypothesis, a 60-day pig manure windrow composting experiment was conducted to investigate the response of CH4 emissions to CaSSP amendment, with a close linkage to methanogenic and methanotrophic communities. Results showed that CaSSP amendment significantly reduced CH4 emissions by 49.5% compared with the control over the whole composting period. The decreased mcrA gene (encodes the α-subunit of methyl-coenzyme M reductase) abundance in response to CaSSP amendment suggested that the CH4 emissions were reduced primarily due to the suppressed microbial CH4 production. Illumina MiSeq sequencing analysis showed that the overall distribution pattern of methanogenic and methanotrophic communities were significantly affected by CaSSP amendment. Particularly, the relative abundance of Methanosarcina that is known to be a dominant group for CH4 production, significantly decreased by up to 25.3% accompanied with CaSSP addition. Only Type I methanotrophs was detected in our study and Methylocaldum was the dominant methanotrophs in this composting system; in detail, CaSSP amendment increased the relative abundance of OTUs belong to Methylocaldum and Methylobacter. Moreover, the increased SO42− concentration and decreased pH acted as two key factors influencing the methanogenic and methanotrophic composition, with the former has a negative effect on methanogenesis growth and can later promote CH4 oxidation at a low level. This study deepens our understanding of the interaction between abiotic factors, function microbiota and greenhouse gas (GHG) emissions, as well as provides implication for practically reducing composting GHG emissions.
The Effect of an Alternative Definition of “Percent Highly Annoyed” on the Exposure–Response Relationship: Comparison of Noise Annoyance Responses Measured by ICBEN 5-Point Verbal and 11-Point Numerical Scales
Authors: Morinaga Makoto , Nguyen Thu Lan , Yokoshima Shigenori , Shimoyama Koji , Morihara Takashi , Yano Takashi
Published: June 2021

Since the development of the 5-point verbal and 11-point numerical scales for measuring noise annoyance by the ICBEN Team 6, these scales have been widely used in socio-acoustic surveys worldwide, and annoyance responses have been easily compared internationally. However, both the top two categories of the 5-point verbal scale and the top three ones of the 11-point numerical scale are correspond to high annoyance, so it is difficult to precisely compare annoyance responses. Therefore, we calculated differences in day–evening–night-weighted sound pressure levels (Lden) by comparing values corresponding to 10% highly annoyed (HA) on Lden_%HA curves obtained from measurements in 40 datasets regarding surveys conducted in Japan and Vietnam. The results showed that the Lden value corresponding to 10% HA using the 5-point verbal scale was approximately 5 dB lower than that of the 11-point numerical scale. Thus, some correction is required to compare annoyance responses measured by the 5-point verbal and the 11-point numerical scales. The results of this study were also compared with those of a survey in Switzerland.
Gender Differences in Prevalence and Risk Factors for Hypertension among Adult Populations: A Cross-Sectional Study in Indonesia
Authors: Defianna Selly Ruth , Santosa Ailiana , Probandari Ari , Dewi Fatwa Sari Tetra
Published: June 2021

Although hypertension is among the main public health concerns in Indonesia, due to the scarcity of data, few studies have investigated the factors associated with hypertension in men and women. This study aimed to examine the prevalence of and factors associated with hypertension among adult men and women in Indonesia. The 2018 Survey of the Sleman Health Demographic and Surveillance System was utilized, consisting of 4328 individuals aged 18+ years. Multivariable logistic regression analysis was performed to determine the sociodemographic and health behavior factors of hypertension. Overall, the prevalence of hypertension was 40% (42% in men and 38% in women). Age, abdominal obesity and chronic non-communicable diseases were the common predictors of hypertension in men and women (p < 0.05). The odds ratio of hypertension among men with low education was lower than among those with high education (OR = 0.52, 95% CI: 0.29–0.94). For women, being in the poorest socioeconomic condition increased the risk of hypertension by 1.67 times compared to the richest (95% CI: 1.21–2.32). Gender differences in the prevalence of and factors associated with hypertension were observed among adult populations in Sleman District, Yogyakarta, Indonesia. Therefore, a gender-based approach in the health prevention strategy to control hypertension for men and women is needed.
Offender Recovery. Forensic Patient Perspectives on Long-Term Personal Recovery Processes
Authors: Møllerhøj Jette
Published: June 2021

Knowledge on user experiences from mentally disordered offenders (MDOs) is still limited in a Danish context, especially regarding recovery from offences, severe mental illness, long-term admissions and often involuntarily contact with hospital psychiatry. The study is based on 34 semi-structured interviews with nine forensic patients exploring their experiences with personal recovery processes. The MDOs point out a significant number of elements and factors enhancing, supporting and limiting personal recovery processes. Long-term recovery processes for MDOs involve coming to terms with mental disorders as well as offences. Working with offender recovery implies addressing and understanding the index offence leading to psychiatric measurement as well as addressing risk and prevention of future crime. This coming to terms is an individual and deeply personal process and it often involves several and changing narratives. According to the informants, professionals play a crucial role in supporting recovery processes and maintaining hope and optimism over time. MDOs experience structural barriers limiting recovery potential, especially stigma or limited areas of participation. It is important not to focus solely on personal recovery as a one-dimensional individual process or responsibility, but as a process also marked by structural and organisational challenges.
Study of Sorption Kinetics and Sorption–Desorption Models to Assess the Transport Mechanisms of 2,4-Dichlorophenoxyacetic Acid on Volcanic Soils
Authors: Cáceres-Jensen Lizethly , Rodríguez-Becerra Jorge , Garrido Carlos , Escudey Mauricio , Barrientos Lorena , Parra-Rivero Jocelyn , Domínguez-Vera Valentina , Loch-Arellano Bruno
Published: June 2021

The sorption behavior of 2,4-dichlorophenoxyacetic acid (2,4-D) in the abundant agricultural volcanic ash-derived soils (VADS) is not well understood despite being widely used throughout the world, causing effects to the environment and human health. The environmental behavior and risk assessment of groundwater pollution by pesticides can be evaluated through kinetic models. This study evaluated the sorption kinetics and 2,4-D sorption–desorption in ten VADS through batch sorption experiments. Differences in the sorption extent for the fast and slow phases was observed through the IPD model where 2,4-D sorption kinetics was controlled by external mass transfer and intra organic matter diffusion in Andisols (C1 ≠ 0). We confirmed from the spectroscopic analysis that the carboxylate group directly drives the interaction of 2,4-D on Andisol soil. The MLR model showed that IEP, FeDCB, and pH×Silt are important soil descriptors in the 2,4-D sorption in VADS. The Freundlich model accurately represented sorption equilibrium data in all cases (Kf values between 1.1 and 24.1 µg1−1/n mL1/ng−1) with comparatively higher sorption capacity on Andisols, where the highest hysteresis was observed in soils that presented the highest and lowest OC content (H close to 0).
Diabetes Mellitus and Its Implications in Aortic Stenosis Patients
Authors: Mourino-Alvarez Laura , Corbacho-Alonso Nerea , Sastre-Oliva Tamara , Corros-Vicente Cecilia , Solis Jorge , Tejerina Teresa , Padial Luis R. , Barderas Maria G.
Published: June 2021

Aortic stenosis (AS) and diabetes mellitus (DM) are both progressive diseases that if left untreated, result in significant morbidity and mortality. Several studies revealed that the prevalence of DM is substantially higher in patients with AS and, thus, the progression from mild to severe AS is greater in those patients with DM. DM and common comorbidities associated with both diseases, DM and AS, increase patient management complexity and make aortic valve replacement the only effective treatment. For that reason, a better understanding of the pathogenesis underlying both these diseases and the relationships between them is necessary to design more appropriate preventive and therapeutic approaches. In this review, we provided an overview of the main aspects of the relationship between AS and DM, including common comorbidities and risk factors. We also discuss the established treatments/therapies in patients with AS and DM.
Integrative Analyses of Circulating Small RNAs and Kidney Graft Transcriptome in Transplant Glomerulopathy
Authors: Kuscu Canan , Kiran Manjari , Mohammed Akram , Kuscu Cem , Satpathy Sarthak , Wolen Aaron , Bardhi Elissa , Bajwa Amandeep , Eason James D. , Maluf Daniel , Mas Valeria , Akalin Enver
Published: June 2021

Transplant glomerulopathy develops through multiple mechanisms, including donor-specific antibodies, T cells and innate immunity. This study investigates circulating small RNA profiles in serum samples of kidney transplant recipients with biopsy-proven transplant glomerulopathy. Among total small RNA population, miRNAs were the most abundant species in the serum of kidney transplant patients. In addition, fragments arising from mature tRNA and rRNA were detected. Most of the tRNA fragments were generated from 5′ ends of mature tRNA and mainly from two parental tRNAs: tRNA-Gly and tRNA-Glu. Moreover, transplant patients with transplant glomerulopathy displayed a novel tRNA fragments signature. Gene expression analysis from allograft tissues demonstrated changes in canonical pathways related to immune activation such as iCos-iCosL signaling pathway in T helper cells, Th1 and Th2 activation pathway, and dendritic cell maturation. mRNA targets of down-regulated miRNAs such as miR-1224-5p, miR-4508, miR-320, miR-378a from serum were globally upregulated in tissue. Integration of serum miRNA profiles with tissue gene expression showed that changes in serum miRNAs support the role of T-cell mediated mechanisms in ongoing allograft injury.
Nucleotide Excision Repair: From Molecular Defects to Neurological Abnormalities
Authors: Krasikova Yuliya , Rechkunova Nadejda , Lavrik Olga
Published: June 2021

Nucleotide excision repair (NER) is the most versatile DNA repair pathway, which can remove diverse bulky DNA lesions destabilizing a DNA duplex. NER defects cause several autosomal recessive genetic disorders. Xeroderma pigmentosum (XP) is one of the NER-associated syndromes characterized by low efficiency of the removal of bulky DNA adducts generated by ultraviolet radiation. XP patients have extremely high ultraviolet-light sensitivity of sun-exposed tissues, often resulting in multiple skin and eye cancers. Some XP patients develop characteristic neurodegeneration that is believed to derive from their inability to repair neuronal DNA damaged by endogenous metabolites. A specific class of oxidatively induced DNA lesions, 8,5′-cyclopurine-2′-deoxynucleosides, is considered endogenous DNA lesions mainly responsible for neurological problems in XP. Growing evidence suggests that XP is accompanied by defective mitophagy, as in primary mitochondrial disorders. Moreover, NER pathway is absent in mitochondria, implying that the mitochondrial dysfunction is secondary to nuclear NER defects. In this review, we discuss the current understanding of the NER molecular mechanism and focuses on the NER linkage with the neurological degeneration in patients with XP. We also present recent research advances regarding NER involvement in oxidative DNA lesion repair. Finally, we highlight how mitochondrial dysfunction may be associated with XP.
Tocopherols and Tocotrienols—Bioactive Dietary Compounds; What Is Certain, What Is Doubt?
Authors: Szewczyk Kacper , Chojnacka Aleksandra , Górnicka Magdalena
Published: June 2021

Tocopherols and tocotrienols are natural compounds of plant origin, available in the nature. They are supplied in various amounts in a diet, mainly from vegetable oils, some oilseeds, and nuts. The main forms in the diet are α- and γ-tocopherol, due to the highest content in food products. Nevertheless, α-tocopherol is the main form of vitamin E with the highest tissue concentration. The α- forms of both tocopherols and tocotrienols are considered as the most metabolically active. Currently, research results indicate also a greater antioxidant potential of tocotrienols than tocopherols. Moreover, the biological role of vitamin E metabolites have received increasing interest. The aim of this review is to update the knowledge of tocopherol and tocotrienol bioactivity, with a particular focus on their bioavailability, distribution, and metabolism determinants in humans. Almost one hundred years after the start of research on α-tocopherol, its biological properties are still under investigation. For several decades, researchers’ interest in the biological importance of other forms of vitamin E has also been growing. Some of the functions, for instance the antioxidant functions of α- and γ-tocopherols, have been confirmed in humans, while others, such as the relationship with metabolic disorders, are still under investigation. Some studies, which analyzed the biological role and mechanisms of tocopherols and tocotrienols over the past few years described new and even unexpected cellular and molecular properties that will be the subject of future research.