bluuebunny/crossref_metadata_2025 huggingface dataset:
Contribuição dos profissionais de saúde no preenchimento da notificação para estudo da violência contra crianças e adolescentes no ambiente hospitalar: um protocolo de revisão de escopo
Authors: Gadelha Joanna Paula Moraes , Garces Thiago Santos , Gondim Raianny de Sousa , Pinto Francisco José Maia
Published: September 2022

O objetivo dessa pesquisa é realizar um protocolo de revisão de escopo para mapear na literatura evidencias disponíveis sobre a contribuição dos profissionais de saúde no preenchimento da ficha de notificação de violência contra criança e adolescente no ambiente hospitalar. Trata-se de um protocolo de pesquisa baseado no manual Joanna Briggs Institute (JBI), seguindo o checklist do Prefered Reporing Items for Systematic Review and Meta-Analyses - Extension for Scoping Reviews (PRISMA-ScR). Será realizada a busca e coleta de dados no mês de outubro de 2022 com os descritores da Ciência da Saúde (DeCS) e Medical Subject Headings (MESH). Os termos localizados serão combinados utilizando-se os operadores booleanos "AND", "OR" ou "NOT", para construção da estratégia de busca.  Seguindo os critérios de elegibilidade, a seleção dos estudos será realizada por dois pesquisadores de forma independente. Posteriormente para a interpretação e analise dos resultados, utilizar-se-á o software SPSS.
Texto lúdico como recurso didático no ensino de ecologia, comportamento e domesticação animal
Authors: Silva Wuglenya Daislla Martins da , Gonsales Beatriz Aparecida Blanco , Torres Pedro Felipe Fróio
Published: September 2022

O ensino de ecologia, comportamento e domesticação animal ainda é um desafio para os profissionais da educação, principalmente em relação às interações interespecíficas entre o ser humano e os animais. Por se tratar de um conteúdo muito teórico, os alunos acabam por visualizá-lo como chato e sem graça. Uma forma de solucionar esse problema é através da utilização de materiais didáticos lúdicos que facilitem a compreensão e a interação dos alunos com o conteúdo. Entretanto, os recursos didáticos sobre o assunto ainda são escassos ou de alto custo. Com isso, esse trabalho tem como objetivo desenvolver um texto discursivo lúdico multidisciplinar como recurso didático de baixo custo no ensino de ecologia e comportamento animal. Para isso foi elaborado um texto lúdico em forma de crônica que possa ser utilizado no ensino fundamental, médio e superior, com base em livros de comportamento animal, biologia geral, ecologia e antropologia, que divirta o leitor com uma narrativa cômica e interativa. O texto conta a história de um rato e as tentativas frustradas de um dos personagens em eliminá-lo de sua casa, descrevendo de forma oculta uma teia trófica antropizada e as relações interespecíficas harmônicas e desarmônicas de comensalismo, predação, cooperação, mutualismo e competição dos personagens, além de suas variações de acordo como comportamento dos mesmos. O recurso didático produzido demonstra ser uma boa ferramenta de ensino, pois proporciona uma forma interativa e divertida de aprender o conteúdo, além de agir como facilitador do diálogo entre o professor e o aluno.
O papel do farmacêutico em uma equipe multidisciplinar oncológica: revisão integrativa
Authors: Nunes Luana Batista , Ferreira Sara Carolline Alves , Santana Wanessa Rodrigues , Sugita Denis Masashi
Published: September 2022

A atuação farmacêutica na oncologia tem sido debatida ao longo dos anos buscando potencializar as contribuições deste profissional junto à equipe multidisciplinar e junto ao paciente. Este trabalho teve como objetivo estudar as percepções profissionais de um profissional farmacêutico atuante em uma área oncológica hospitalar. A metodologia utilizada trata-se de uma entrevista estruturada realizadas por meio remoto, contendo perguntar abertas que deixam o entrevistado livre para responder abertamente sobre sua atuação. Em relação aos resultados obtidos, destaca-se que todas as perguntas foram respondidas e indicam, de forma geral, que a atuação da profissional segue em um contexto que demanda conhecimento, preparo técnico, emocional, além de uma atuação humanizada. Conclui-se que o farmacêutico na área oncológica contribui significativamente para a obtenção de um cenário mais favorável à melhora do paciente. Sua atuação vais desde a preparação dos medicamentos até o contato direto com os pacientes para esclarecer dúvidas e tranquilizar sobre os fármacos que estão sendo utilizados.
Fatores fundamentais que motivam os bacharéis em engenharia a se tornarem docentes no Brasil
Authors: Neves Thuanny Reis , Albrecht Evonir
Published: September 2022

No cenário atual, é notório que uma parcela considerável dos Bacharéis em Engenharia opta por seguir o caminho da docência. Neste contexto, o presente trabalho tem por objetivo discorrer sobre os principais fatores que motivam os Bacharéis em Engenharia a se interessarem por seguir o caminho da docência no Brasil. Até mesmo alguns profissionais que já possuem uma carreira consolidada no ramo da Engenharia, em algum momento passam a exercer a atividade docente em paralelo com sua primeira área de atuação. Alguns recém-formados, também buscam trilhar os caminhos da docência, mesmo sem terem ingressado no mercado de trabalho como engenheiros, tanto pela dificuldade de conseguirem uma oportunidade de se estabelecer no ramo, quanto por se identificarem mais com o segmento educacional. Outro fator fundamental, nesse contexto, é a crescente implementação de cursos técnicos, tecnólogos e de graduação no Brasil, os quais demandam cada vez mais profissionais formados em Engenharia para ministrarem grande parte das disciplinas técnicas que compõem a grade curricular desses cursos.
O uso de ingredientes alternativos na ração para tambaqui (Colossoma macropomum): uma revisão
Authors: Cornélio Joana Paula de Souza
Published: September 2022

A utilização de ingredientes não convencionais é uma opção para reduzir custos na produção de rações para tambaqui (Colossoma macropomum) que atualmente é a espécie nativa mais produzida no Brasil. Assim, o objetivo deste estudo foi avaliar o estado da arte das pesquisas que tratam sobre o uso de ingredientes alternativos em substituição aos convencionais na alimentação do tambaqui, identificando os principais produtos da biodiversidade brasileira utilizados, bem como as vantagens e desvantagens da inclusão desses ingredientes na ração para essa espécie. Foi realizada uma revisão narrativa da literatura sobre o tema abordado, buscando-se artigos científicos nas principais bases de dados (Scielo e Scopus), além da ferramenta do Google Acadêmico, disponíveis de forma digital, no período de 2010 a 2022. Os resultados demonstraram que o uso de ingredientes alternativos na dieta do tambaqui em substituição aos ingredientes convencionais foi promissor na maioria dos trabalhos revisados, excetuando-se a torta de cupuaçu; polpas de abacaxi, manga e maracujá; farinha de banana e óleo essencial de gengibre cujas inclusões nas rações interferiram de forma negativa nos índices avaliados. Dos ingredientes alternativos utilizados nas pesquisas 93,75% foram de origem vegetal, principalmente frutos amazônicos. Foram utilizados somente juvenis nas pesquisas e apenas 31,25% dos trabalhos determinaram os coeficientes de digestibilidade. Assim, novas pesquisas devem ser direcionadas visando encontrar uma melhor combinação dos ingredientes alternativos e o melhor equilíbrio dos nutrientes, em especial para peixes acima de 125 g.
Computer simulation of mechanical strength compared between straight and wave plates for femoral application through finite elements
Authors: Vargas Rogério Santos , Araldi Jonatas Comparin , Bringmann Deise Renata , Falavigna Asdrubal , Corso Leandro Luis
Published: September 2022

The objective of this study was to evaluate, by means of computer simulation, the difference in mechanical strength between two plate models  straight and wave  used in femur fracture fixation, submitting both to a progressive static axial load. There are criteria to evaluate the yield strength of a material: Tresca, Von Mises and Mohr-Coulomb. In this study, the Von-Mises strain criterion theory was used because it is used in fatigue strength tests of ductile materials, in this case, stainless steel. This criterion, indicates that the yielding of a solid material begins when it reaches a critical stress value. The models were built computationally using 3D modeling software. The finite element mathematical method was used to evaluate the stress and strain curve, two elements considered fundamental to verify the behavior of the metal during the application of stress and the displacement of the plates, to thus evaluate the strength of each. The results obtained after the finite element analysis show that the plates do not reach the critical limit for yielding, but the straight plate absorbs 10 times more stress compared to the wave plate. The wave plate allows the conclusion that there is decomposition of the applied force. Both plates remain in the elastic regime with load up to 1000 N. The load is equivalent to the weight of the body and gravity. It was concluded that the axial force applied in the caudal direction decomposes into resulting ones compared to the straight plate, which absorbs all the load and may reach the critical limit for yielding before the wave plate.
The Power Wars of Cleopatras of Ptolemaic Origin, Who Attended the Seleucid Kingdom Through Marriage
Authors: TEMİZKAN Meltem
Published: September 2022

Marriages arranged to support political alliances between Hellenistic kingdoms were a symbol of peace between dynasties. These marriages were especially applied between the two important kingdoms of the Hellenistic world, the Seleucid and Ptolemaic dynasties. With diplomatic marriages, the Seleucid and Ptolemaic kingdoms aimed to make the peace process permanent. In the peace text that will come into effect after the Second Syrian War (260-253 BC) between the Seleucid and Ptolemaic kingdoms, the marriage to be realized between these two dynasties was reflected in the text of the treaty and the Seleucid King II. Antiochos Theos and Ptolemaic Princess Berenike Phernophoros formalized their marriage in 252 BC. With the political marriages between the Seleucid and Ptolemaic kingdoms, the peace establishment was tried to be preserved and consolidated. Cleopatras of Ptolemaic origin took place in the Seleucid Dynasty and assumed an active role in the kingdom, with Cleopatra Thea as of 150 BC. The increasing dominance of Queen Cleopatra Thea with her attempts to gain political power in the Seleucid Kingdom showed that Cleopatras of Ptolemaic origin tried to be permanent and active. Cleopatra Trypheana, Cleopatra IV and Cleopatra V Selene, as Seleucid queens of Ptolemaic origin, entered into a struggle for existence with a series of political and social activities in the Seleucid Dynasty. In this study, the political existence struggles of the Ptolemaic origin Cleopatras in the Seleucid Kingdom were examined, and it was aimed to analyze the effects of these activities on the Seleucid Kingdom in line with ancient and modern sources.
Assessments About the Scythian Origin of Arsaces I, Founder of the Parthian (Arsacid) State
Authors: DEMİR Muzaffer
Published: September 2022

Orta Asya konargöçerleri -Daai, Massagetai ve Sakai- MÖ 331-330 yılları civarında III. Dareios’un ve hatta sonrasında Büyük İskender’in ordusunda özel atlı okçu birlikler olarak hizmet etmişlerdir. Büyük İskender’in MÖ 323 yılında ani ölümü İran’ın kuzey doğu coğrafyasını da ciddi bir şekilde istikrarsızlığa sürüklemiştir. Daai Konfederasyonu’na bağlı üç kabileden biri olan ve İskit kökenli A/parnoi, B. İskender’in bölge üzerinde hâkimiyet kurmuş olan varislerinden I. Seleukos (MÖ 305-281)’un iktidarı döneminde kuzeydeki Khorasmia (Harezm) bölgesinden güneye doğru özellikle Margiana (genel hatlarıyla Türkmenistan) üzerine saldırılarını yoğunlaştırmış ve tam anlamıyla geri püskürtülememiştir. II. Seleukos (MÖ 246-226/5)’un tahta çıkar çıkmaz batıda yüzleşmek zorunda kaldığı iç ayaklanma ve komşularının saldırılarına karşı başarısız olması doğuda Baktria ve Parthia valilerinin de ayaklanmasına yol açmıştır. Bunu fırsat bilen A/parnoi’nin başındaki karizmatik lideri I. Arsakes, önce Baktria valisinin kontrolündeki Margiana’ya saldırmış ancak geri püskürtüldüğünden Parthia (Horasan) bölgesine yönelmiş ve bağımsızlığını yeni ilan etmiş olan bölge valisi Andragoras’ı öldürerek İran’da Parth devletinin temellerini atmıştır. Parth devletinin kuruluş süreci hakkında çok sayıda modern literatür bulunurken, I. Arsakes’in kökeni hakkında şimdiye kadar detaylı bir çalışmanın yapılmadığı görülmektedir. I. Arsakes’in kökeniyle ilgili verdikleri bilgiler günümüze kadar ulaşan dört antik yazar bulunmaktadır: Strabon, Iustinus (Trogus), Marcellinus ve Arrianus (Photios ve Synkellos). Biz bu yazarların ilgili metinlerini karşılaştırmalı olarak analiz ederek I. Arsakes’in İskit kabilelerinden A/parnoi’nin soyundan geldiği tezini destekleyeceğiz. Bu kapsamda A/parnoi kabilesinin İran’a gelmeden önce çıktığı Khorasmia (Harezm), Hyrkania (Dahistan) ve Margiana coğrafyaları, daha sonraki Parth krallarının özellikle Margiana coğrafyasına bağlılıklarını devam ettirmeleri ve Parthların yaşam tarzları, savaş taktikleri ve geleneklerinin kuzeydeki İskit halklarınınkine benzemesi temaları üzerine odaklanacağız.
GALT Enzyme Deficiency Identification in Newborns in Azerbaijan
Authors: Hajiyeva N.
Published: September 2022

For the first time in Baku, Azerbaijan Republic in maternity houses by means of immunoenzyme analysis the genetic screening for galactosemia inherited metabolism disorder was carried out, and 3 newborns with GALT gene deficiency for were identified.
Dynamics of Hormones of the Pituitary-Thireoid System and EEG of Brain Activity in High-Mountain Residents
Authors: Sadykova G. , Dzhunusova G.
Published: September 2022

The article presents an analysis of the results of studies to determine the functional state of the pituitary-thyroid system and to identify their relationship with the neurodynamic EEG parameters of the brain in mountainous conditions (2800m). The results of enzyme-linked immunosorbent assays of blood in mountain dwellers indicate functional activation (according to average values of T4 and T3 levels) of the peripheral link and compensatory calmness of the central link (relatively low levels of TSH) of the pituitary-thyroid system. A decrease in TSH secretion at a normal level of thyroxine indicates a trend towards a decrease in the functional state of the thyroid gland, which confirms the presence of adaptive changes in the body of mountaineers. At the same time, the detected normal (or with a slight deviation in one direction or another) thyroxine leveldoes not at all contradict the general pattern, because reflects the variability inherent in biological phenomena, due to the individual characteristics of the organism, and the dependence of the studied indicator on factors other than hypoxic factors. Due to the high tension of the functioning of the central nervous system in high altitude conditions, there is a peculiar shift in the EEG parameters towards a decrease in the alpha rhythm and an increase in the theta rhythm of tension, which causes a noticeable redistribution of typological groups. The type of CMR of the brain on the EEG has close functional relationships with the level of TSH, and also has a weak functional relationship with the levels of thyroid hormones.
Focus on Cardiotoxicity and Its Prevention in Patients With Cancer Diseases
Authors: Akbalaeva B. , Batyraliev T. , Tursunbaev M. , Kalmatov R. , Mamatova S.
Published: September 2022

Research relevance: currently, in many less developed countries, including Kyrgyzstan, cardioprotection has not previously been performed in cancer patients receiving chemotherapy, which should be given special attention. If patient has a pre-existing cardiovascular disease, then the heart disease should first be corrected before chemotherapy or radiotherapy is started. Research objectives: determination of cardio and antitumor drugs effect on cardiotoxicity and cardioprotective efficacy of some beta-blockers when used in patients undergoing chemotherapy. Research materials and methods: risk assessment of cardiotoxicity drugs used in chemotherapy, classification of cardiotoxicity and cardiovascular toxicity according to signs. Research results: considering the growth of malignant diseases, this industry needs to be developed in order to improve patients’ quality of life, as well as to reduce the mortality of the latter from cardiovascular complications associated with toxic myocardial damage. Conclusion: cardio-oncology is a really new and promising specialty that requires large, randomized trials in our country.
Changes in the Functional State of the Cardiovascular System in the Middle, Elderly and Senile Age Depending on the Season
Authors: Zhalalova G. , Zholdoshev S. , Mamatkulova N.
Published: September 2022

The article reflects the study of the influence of climatic and seasonal factors on the body of people of middle, elderly and senile age. These factors significantly aggravate the course of many diseases, worsen health and reduce the body's performance. The incidence of cardiovascular disease is often associated with risk factors and seasons. Most people aged 65 years and older often suffer from hypertension. With age, the number of such patients increases significantly. Severe hypertension is rare in humans because patients with hypertension do not live to an advanced age or often develop complications. Changing seasonal factors requires great attention to the functional state of the cardiovascular system. Patients with hypertension are very difficult to adapt to changing weather conditions, which worsens the quality of life of the elderly and leads to various complications. Changes in blood pressure in people of middle, elderly and senile age depending on the season of the year have been established.
Features of Allergic Diseases in the Osh Region
Authors: Boronchieva Z. , Aldashukurov Y.
Published: September 2022

In this paper, the subject of research is allergic diseases. The aim of the study was to study the frequency of visits to the Osh Interregional Joint Clinical Hospital for allergies. The study of this problem is of considerable interest. Currently, it is difficult to find a person without symptoms of allergy, because every fourth person is a carrier of it. The presence of a huge number of people suffering from this disease is due to the action of many factors, in particular: poor ecology, heredity, the impact of constantly irritating factors. According to the results of epidemiological studies, on average about 10% of the world’s population suffer from various forms of allergic pathology and only an increase in the number of these diseases is expected in the future. According to WHO, currently more than 5% of the world's adult population and 15% of children suffer from allergic diseases. In Kyrgyzstan, every fourth citizen suffers from some form of allergy. And in ecologically unfavorable regions, the level of allergic diseases among the population reaches 30-60%. The results of the conducted studies have shown that the frequency of referrals with allergic diseases increases in all age categories. The structure of allergic diseases is dominated by allergic rhinitis — 48.1%, urticarial — 20.5% and atopic dermatitis allergies — 18.3%.
Ambulance in Kyrgyzstan and Abroad (literature review)
Authors: Ismailova F. , Karataev M.
Published: September 2022

This article presents the literature review on medical emergency ambulance service. The problems related to the organizing and improving the medical emergency ambulance service continue to be significant and relevant in modern world that requires further studying. The author has conducted the historical analysis of the literature sources of the medical emergency ambulance service in different countries of the world. The effectiveness of various forms of the emergency medical care at pre-hospitalization and hospitalization stages are studied. One of the areas in healthcare that needs improvement today is the advancement of the emergency medical care systems. The problems related to the ambulance service of Osh city are highlighted. Examining the emergency medical care (organizational forms) outside the Kyrgyz Republic makes it possible to compare the advantages and disadvantages of the service and can contribute to its improvement in Osh, considering the experience of foreign countries. To reduce the workload of the medical emergency ambulance service, the need for optimizing the emergency medical care at the centers of family medicine is determined. The literature review methods that was used in this article are from the databases of the elibrary, cyberleninka, Medline on the organization of the medical emergency ambulance service in different countries of the world.
Underwater Robot Technology and Future Trend Analysis
Authors: Liang Chuanming
Published: September 2022

Underwater positioning and navigation is a technology that provides the attitude, speed, position and other information of underwater robots. It is the premise for underwater robots to successfully complete various underwater tasks. The underwater environment is complex and changeable, and a single positioning and navigation technology will inevitably not meet the needs of long-range, multi-target and high-precision. The navigation system of the future underwater intelligent robot integrates the integrated navigation mode of various technologies. This paper discusses the basic underwater positioning and navigation mode and integrated navigation mode, describes the development process of underwater positioning and navigation technology, compares the research methods and progress of Chinese and foreign scholars, analyzes the current research bottlenecks and technical difficulties, and points out the direction for future research, which can be used as a reference for the follow-up research of underwater robot positioning and navigation system.
Botulinum Toxin Type A Application Experiences in Bolu Abant İzzet Baysal University Neurology Clinic
Authors: AKÜNAL TÜREL Canan
Published: September 2022

Aim: The aim of this study was to evaluate the effects of different clinical applications of BoNT-A as hemifacial spasm, blepharospasm, cervical dystonia, bruxism, head tremor and spasticity in stroke, Multiple Sclerosis (MS), spinal trauma, hereditary spastic paraplegia, cerebral palsy, and chronic migraine.

Material and Methods: In this retrospective study, which was conducted between January 2019 and December 2021, 79 patients with movement disorders, spasticity, and chronic migraine monitored in our clinic and whose BoNT-A injections were applied were included. The diagnosis and treatment, side effects encountered in therapy, follow-up during the treatment, and the response to the treatment evaluated subjectively by patients were discussed.

Results: The improvement in patients' response to treatment was subjectively reported by themselves. The improvement in the response of the patients to the treatment subjectively stated that improvement of 60% and above was taken as good, improvement between 59-40% moderate, and improvement of 39% and below bad. Accordingly, 78.94% of the patients subjectively stated improvement as good, 13.15% as moderate, and 7.89% as bad. Although the incidence of complications was 9.21%, these side effects were mild and transient in all of the patients and did not cause any of the patients to discontinue the treatment.

Conclusion: The results were statistically significant and consistent with the literature. This study supports the fact that botulinum toxin therapy is effective and safe for HS, BS, cervical dystonia, spasticity, bruxism, head tremor, and chronic migraine.
Çocuklarda Bitki ve Bitkisel Ürün Kullanımlarının Araştırılması
Authors: AKKUŞ Ayşe , MANCAK KARAKUŞ Methiye , KOCA ÇALIŞKAN Ufuk
Published: September 2022

Amaç: Bu çalışmada, son yıllarda tüm yaş gruplarında bitkisel ürünlere yönelimin artmasından yola çıkarak, çocuk polikliniğine başvuran çocukların ve ebeveynlerinin bitkisel ürün kullanımının araştırılması ve değerlendirilmesi amaçlanmıştır.

Gereç ve Yöntemler: Anket çalışması, Gülhane Eğitim ve Araştırma Hastanesi Çocuk Hastalıkları Polikliniği’ne başvuran 200 ebeveynle yüz yüze görüşülerek gerçekleştirilmiştir. Katılımcıların demografik özellikleri ve bitkisel ürün kullanımı, sağlık personeline danışma durumları, bitkisel ürünler ile ilgili düşünceleri hakkında bilgi edinilmiş ve istatistiksel analizlerle değerlendirilmiştir.

Bulgular: Anket sonuçlarına göre çocukların 36’sının (%18) kronik hastalığı olup 31’inin (%86) tedavi amaçlı bitkisel ürün kullandığı tespit edilmiştir. Ebeveynlerin 162’si (%81), çocukları için en çok soğuk algınlığı, öksürük, gaz sancısı tedavisi için bitki veya bitkisel ürün uygulamış ve bu ürünleri çoğunlukla aktarlardan temin etmiştir. Bitkisel ürün kullanımı erkek ebeveynlerde, kadınlara göre daha yüksek olup aynı rahatsızlığı çeken kişilerin fayda görmesi aileler tarafından önemli bir kriter olarak göz önünde bulundurulmuştur. Bitkisel ürün tercihlerinde arkadaş ve akraba tavsiyeleri etkili olmuştur. Ailelerin 86’sının (%53) hekim veya sağlık personeline danışmadan bitkisel ürünleri kullandığı, 134’ünün (%82,5) bitkisel ürünleri kullanmadan önce güvenli olup olmadığını araştırdığı, 129’unun (%80) bitkisel ürünlerden fayda gördüğünü ifade ettiği, 96’sının (%59) ise bitkisel yöntemlerin etkin ve güvenilir olduğunu düşündüğü anket sonucunda tespit edilmiştir.

Sonuç: Bitkisel ve doğal ürünlerle tedavi yöntemlerinin tıbbi ilaçlardan daha doğal ve yan etkisinin daha az olduğunun aileler tarafından ifade edildiği görülmüş olup, konu ile ilgili hekim veya sağlık personeline verilecek eğitimler ile ailelerin daha doğru yönlendirilebileceği düşünülmektedir.

Anahtar Kelimeler: Fitoterapi; çocuklar için fitoterapötikler; pediatri; geleneksel ve tamamlayıcı tıp; bitkisel tedavi.
B.1.1.7 SARS CoV-2 Varyantına Bağlı COVID-19 Tanısıyla Takip Edilen Hastaların Değerlendirilmesi
Authors: KAVAK Şeyhmus , KAYA Şafak , RAHMANALİ ONUR Arzu , KUBAT Gülnaz
Published: September 2022

Amaç: COVID-19’un etkeni olan SARS-CoV-2’nin replikasyonu sırasında sayısız varyantlar ortaya çıkmaktadır. İki bin yirmi yılının Eylül ayında İngiltere’de ortaya çıkan B.1.1.7 (İngiltere) varyantı da bunlardan biridir. Bu çalışmada B.1.1.7 varyantına bağlı gelişen COVID-19 nedeniyle takip edilen hastaların verilerini gözden geçirmeyi amaçladık.
Gereç ve Yöntemler: Bu çalışma B.1.1.7 varyantının neden olduğu COVID-19 tanısıyla takip edilen toplam 50 hastanın verilerini içeren retrospektif bir çalışmadır. Hastalar yaş, cinsiyet, altta yatan hastalık, semptomlar ve laboratuar bulguları açısından incelendi.
Bulgular: Çalışmaya alınan 50 hastanın 20’si (%40) kadın ve yaş ortalaması 59,4±16,3 idi. Onbir (%22) hastada lökositoz, dört (%8) hastada lökopeni, 24 (%48) hastada lenfopeni, 42 (%84) hastada C reaktif protein artışı, 28 (%56) hastada ferritin yüksekliği, 32 (%64) hastada laktat dehidrogenaz yüksekliği, 16 (%32) hastada d-dimer yüksekliği mevcuttu. Hastaların 45 (%90)’inde toraks bilgisayarlı tomografide tutulum mevcuttu. Grup 1 (n=36) ve grup 2 (n=14) karşılaştırıldığında, iki grup arasında lökosit, d-dimer ve prokalsitonin düzeyleri arasında istatistiksel olarak anlamlı fark mevcuttu (p değerleri sırasıyla p˂0,000, p=0,001, p=0,001).
Sonuç: Sonuç olarak, B.1.1.7 varyantına sahip hastaları irdelediğimiz bu çalışmada ölen hastalarda lökosit, prokalsitonin ve d-dimer değerleri sağ kalan hastalara göre istatistiksel olarak anlamlı düzeyde daha yüksekti. Bu hastalardan elde ettiğimiz veriler varyant olmayan hastaların literatür verilerine benzerdi.
Investigation of Neutrophil Volume, Conductivity, and Light-Scattering Parameters for Early Diagnosis of Bacterial Infections
Authors: DOĞAN Özlem , ÇALIŞKAN Emel , ALTINÖZ AYTAR Asiye
Published: September 2022

Aim: Early diagnosis of bacterial infections is crucial in planning treatment. Thus, it is important to determine the gram staining property of bacteria as well as the presence of bacterial infection. The acute bacterial infection leads to morphological changes in neutrophils. In this study, we investigated the use of neutrophil volume, conductivity and light-scattering (VCS) parameters as potential indicators for rapid diagnosis of bacterial infections, and to distinguish infections caused by gram-positive and gram-negative bacteria.

Material and Methods: Patients with urinary tract infections, pneumonia, wound site infections and sepsis were included. The control group comprised patients without bacterial infections. The blood samples of the patients were examined for white blood cell and neutrophil counts; neutrophil VCS parameters were determined using a Coulter Analyzer. The VITEK 2 Compact System was used to detect microbial growth.

Results: The blood sample data of 472 patients sent to our laboratory for Complete Blood Count analyses from various clinics were analyzed in this study. A total of 370 samples showed significant growth in their bacteriological culturing, whereas the remaining 102 samples showed no growth. For the detection of bacterial growth, the specificities of median neutrophil volume (MNV) and median neutrophil conductivity (MNC) were found to be 96% and 99%, respectively. In addition, median neutrophil light scattering (MNS) was higher in the gram-negative bacterial group than in the gram-positive bacterial group.

Conclusion: The use of neutrophil VCS parameters is an effective and time-saving method to identify bacterial infections and distinguish between gram-positive and gram-negative bacterial infections.
Diyabetik Nöropatinin Yönetiminde Güncel Tedavi Yaklaşımları ve Hemşirelik Bakımı
Authors: GÖRGÜLÜ Ümit , ÇİFTÇİ Selin , POLAT Ülkü
Published: September 2022

Diyabetik nöropati bireylerin yaşam kalitesini etkileyen ve morbidite riski yüksek olan diyabetin en sık görülen nörolojik komplikasyonlarından biridir. Diyabetli birçok kişi, diyabetik nöropati ile ilişkili semptomları veya sorunları bildirmez. Diyabeti olan bireyler, nöropatiye özgü öykü, tarama testi ve fizik muayene yapılarak yıllık olarak taranmalıdır. Bu bireyler kötü yaşam kalitesi, nöropatik ağrı, düşmeler, ayak ülseri gelişimi ve enfeksiyonlar, ampütasyon açısından yüksek risk altındadır. Komplikasyonları en aza indirmek ve yaşam kalitesini iyileştirmek için diyabetik nöropatili bireylerin erken aşamada tanılanması, geçerli ve güvenilir tarama araçları ile değerlendirilmesi ve diyabetik nöropatinin yönetimi önemlidir. Diyabetik nöropatinin yönetimi multidisipliner ekip yaklaşımını gerektirir. Bu ekibin önemli bir üyesi olan hemşirelerin sorumluluklarından bazıları diyabetik nöropatili ve nöropati gelişme riski olan bireylerde farkındalığı artırmak, yüksek riskli gruplar için müdahaleler planlamak, doğru bilgi ve davranış kazanmalarını sağlamaktır. Hemşirenin diyabetik nöropatili bireylerin taranması, teşhisi, tedavisi ve bakım uygulamaları konusunda farkında ve bilgili olması olumsuz sonuçların gelişmesini önlemede veya azaltmada yarar sağlar.
Analysis of Traditional Menu Promotion Case Study: The City of Pagar Alam
Authors: Samuel Hamonangan  , Pelliyezer Karo Karo  , Gus Setiawan
Published: September 2022

This study compares traditional menu promotion efforts' expectations and reality in Pagar Alam, South Sumatra. The focus of attention of food and beverage industry players is customers and competitors. By generating and offering value to their customers, food and beverage industry players can bind a customer, a food and beverage player who has succeeded in presenting superior value. In order to achieve this goal, every industry player must be able to produce and deliver the goods and services desired by consumers, in this case, tourists, according to the needs and desires of these tourists. The data analysis method uses Important Performance Analysis (IPA) on primary data obtained from 83 consumer respondents. The results showed strategies to improve product branding to introduce and improve the specific menu products of natural fences so that tourists more widely knew them. The results showed that labeling was in quadrant D (low expectation and perception values) at an IPA value of 5.40.
Implementation of the concept of tax expenditures in Ukraine: problems and ways to solve them
Authors:   SESE “The Academy of Financial Management”, SOKOLOVSKA Alla
Published: September 2022

Introduction. The preparation of reports on tax expenditures and their use in the process of budget management has become widespread in many countries of the world. Since 2004, the Global Tax Expenditure Database has included information on tax expenditures in Ukraine. However, the degree of implementation of the concept of tax expenditures in the budget process varies greatly in different countries. Problem Statement. Imperfect procedures for reporting on tax expenditures and their formal connection with the budget process, or even the absence of such a connection at all are significant reasons for insufficient transparency of tax expenditures budgets and the effectiveness of their use as public policy tools. Purpose. The goal is to assess the state, determine the problems of integrating the report on budget losses from the use of tax reliefs (tax expenditures) into the budget process in Ukraine and ways to solve them. Methods. General scientific methods of cognition were used: critical analysis of the controversial provisions of the concept of tax expenditures; a combination of abstract-logical and concrete, comparative analysis. Results. The steps that should be taken to ensure the effective implementation of the tax expenditure budget, as well as the state of their implementation in several countries and in Ukraine, are analyzed. The problems on the way to the implementation of the concept of tax expenditures in Ukraine are revealed: uncertainty at the legislative level of the concept of “tax expenditures” and the body responsible for the formation of a consolidated report on tax expenditures; non-disclosure of their assessment methodology; improper coordination of the work of the State Tax Service of Ukraine and the State Customs Service of Ukraine on collecting and transmitting information on tax expenditures; formal inclusion of information on tax expenditures in the budget process; absence of systematic evaluation of the effectiveness and efficiency of tax expenditures. Conclusions. It has been established that only the first steps have been taken in Ukraine towards the development and implementation of the tax expenditure budget and its integration into the country's budget process, and proposals have been developed for measures that must be implemented for further progress on this path, in particular changes to tax and budget legislation, as well as the administrative documents of executive authorities involved in the preparation of reports on tax expenditures.
“Motile-targeting” drug delivery platforms based on micro/nanorobots for tumor therapy
Authors: Zhang Di , Liu Shuyi , Guan Jianguo , Mou Fangzhi
Published: September 2022

Traditional drug delivery systems opened the gate for tumor-targeted therapy, but they generally took advantage of enhanced permeability and retention or ligand-receptor mediated interaction, and thus suffered from limited recognition range (<0.5 nm) and low targeting efficiency (0.7%, median). Alternatively, micro/nanorobots (MNRs) may act as emerging “motile-targeting” drug delivery platforms to deliver therapeutic payloads, thereby making a giant step toward effective and safe cancer treatment due to their autonomous movement and navigation in biological media. This review focuses on the most recent developments of MNRs in “motile-targeting” drug delivery. After a brief introduction to traditional tumor-targeted drug delivery strategies and various MNRs, the representative applications of MNRs in “motile-targeting” drug delivery are systematically streamlined in terms of the propelling mechanisms. Following a discussion of the current challenges of each type of MNR in biomedical applications, as well as future prospects, several promising designs for MNRs that could benefit in “motile-targeting” drug delivery are proposed. This work is expected to attract and motivate researchers from different communities to advance the creation and practical application of the “motile-targeting” drug delivery platforms.
3D-printed Sr2ZnSi2O7 scaffold facilitates vascularized bone regeneration through macrophage immunomodulation
Authors: Pan Hao , Deng Li , Huang Lingwei , Zhang Qi , Yu Jing , Huang Yueyue , Chen Lei , Chang Jiang
Published: September 2022

Biomaterial-based bone grafts are emerged as an effective strategy for the treatment of large bone defects, especially for the scaffolds with enhanced osteogenic and angiogenic bioactivities. However, most studies focused on the direct interactions between scaffolds and bone-related cells such as osteoblasts and endothelial cells, and ignored the effects of material-triggered immunomodulation and the subsequent immune-regulated bone regeneration process. In this study, we developed a silicate bioceramic (Sr2ZnSi2O7, SZS) scaffold with well-defined pore structures using a three-dimensional (3D) printing technique. The prepared scaffolds were biodegradable, and the released bioactive ions were beneficial for immunomodulation, which stimulated macrophages to release more pro-healing cytokines and less pro-inflammatory cytokines. The obtained scaffold/macrophage conditioned medium further promoted the proliferation and osteogenic differentiation of a murine preosteoblast cell line (MC3T3-E1), as well as the angiogenic activity of human umbilical vein endothelial cells (HUVECs). Moreover, the in vivo experiments of critical-sized calvarial defects in rats revealed that the 3D printed SZS scaffolds could facilitate more vascularized bone regeneration than the 3D printed β-tricalcium phosphate (β-TCP, a typical clinically used bioceramic) scaffolds, suggesting that the 3D-printed SZS scaffolds hold the potential as implantable biomaterials with favorable osteoimmunomodulation for bone repair.
Discovery of novel secretome CAZymes from Penicillium sclerotigenum by bioinformatics and explorative proteomics analyses during sweet potato pectin digestion
Authors: Barrett Kristian , Zhao Hai , Hao Pengfei , Bacic Antony , Lange Lene , Holck Jesper , Meyer Anne S.
Published: September 2022

Novel selective enzymatic refining of sweet potato processing residues requires judicious enzyme selection and enzyme discovery. We prepared a pectinaceous cell wall polysaccharide fraction from sweet potato using an enzymatic a treatment to preserve the natural linkages and substitutions. Polysaccharide composition and linkage analysis data confirmed the pectinaceous polysaccharide fraction to be a rhamnogalacturonan I-rich fraction with a high content of arabinogalactan Type I. We hypothesized that the post-harvest tuber pathogenic fungus Penicillium sclerotigenum would harbor novel enzymes targeting selective sweet potato pectin modification. As part of the study, we also report the first genome sequence of P. sclerotigenum. We incubated the sweet potato pectinaceous fraction with P. sclerotigenum. Using proteomics accompanied by CUPP-bioinformatics analysis, we observed induced expression of 23 pectin-associated degradative enzymes. We also identified six abundantly secreted, induced proteins that do not correspond to known CAZymes, but which we suggest as novel enzymes involved in pectin degradation. For validation, the predicted CUPP grouping of putative CAZymes and the exo-proteome data obtained for P. sclerotigenum during growth on sweet potato pectin were compared with proteomics and transcriptomics data reported previously for pectin-associated CAZymes from Aspergillus niger strain NRRL3. The data infer that P. sclerotigenum has the capacity to express several novel enzymes that may provide novel opportunities for sweet potato pectin modification and valorization of sweet potato starch processing residues. In addition, the methodological approach employed represents an integrative systematic strategy for enzyme discovery.
Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance
Authors: Huang Ying , Ma Huijun , Wang Xiaodong , Cui Tianxiang , Han Gang , Zhang Yu , Wang Chao
Published: September 2022

Plant nuclear factor-Y (NF-Y) transcription factors (TFs) are key regulators of growth and stress resistance. However, the role of NF-Y TFs in poplar in response to biotic stress is still unclear. In this study, we cloned 26 PdbNF-Y encoding genes in the hybrid poplar P. davidiana × P. bollena, including 12 PdbNF-YAs, six PdbNF-YBs, and eight PdbNF-YCs. Their physical and chemical parameters, conserved domains, and phylogeny were subsequently analyzed. The protein–protein interaction (PPI) network showed that the three PdbNF-Y subunits may interact with NF-Y proteins belonging to two other subfamilies and other TFs. Tissue expression analysis revealed that PdbNF-Ys exhibited three distinct expression patterns in three tissues. Cis-elements related to stress-responsiveness were found in the promoters of PdbNF-Ys, and most PdbNF-Ys were shown to be differentially expressed under Alternaria alternata and hormone treatments. Compared with the PdbNF-YB and PdbNF-YC subfamilies, more PdbNF-YAs were significantly induced under the two treatments. Moreover, loss- and gain-of-function analyses showed that PdbNF-YA11 plays a positive role in poplar resistance to A. alternata. Additionally, RT‒qPCR analyses showed that overexpression and silencing PdbNF-YA11 altered the transcript levels of JA-related genes, including LOX, AOS, AOC, COI, JAZ, ORCA, and MYC, suggesting that PdbNF-YA11-mediated disease resistance is related to activation of the JA pathway. Our findings will contribute to functional analysis of NF-Y genes in woody plants, especially their roles in response to biotic stress.
Microbes of traditional fermentation processes as synthetic biology chassis to tackle future food challenges
Authors: Ramírez Rojas Adán Andrés , Swidah Razan , Schindler Daniel
Published: September 2022

Microbial diversity is magnificent and essential to almost all life on Earth. Microbes are an essential part of every human, allowing us to utilize otherwise inaccessible resources. It is no surprise that humans started, initially unconsciously, domesticating microbes for food production: one may call this microbial domestication 1.0. Sourdough bread is just one of the miracles performed by microbial fermentation, allowing extraction of more nutrients from flour and at the same time creating a fluffy and delicious loaf. There are a broad range of products the production of which requires fermentation such as chocolate, cheese, coffee and vinegar. Eventually, with the rise of microscopy, humans became aware of microbial life. Today our knowledge and technological advances allow us to genetically engineer microbes - one may call this microbial domestication 2.0. Synthetic biology and microbial chassis adaptation allow us to tackle current and future food challenges. One of the most apparent challenges is the limited space on Earth available for agriculture and its major tolls on the environment through use of pesticides and the replacement of ecosystems with monocultures. Further challenges include transport and packaging, exacerbated by the 24/7 on-demand mentality of many customers. Synthetic biology already tackles multiple food challenges and will be able to tackle many future food challenges. In this perspective article, we highlight recent microbial synthetic biology research to address future food challenges. We further give a perspective on how synthetic biology tools may teach old microbes new tricks, and what standardized microbial domestication could look like.
Formation of sweet potato starch nanoparticles by ultrasonic—assisted nanoprecipitation: Effect of cold plasma treatment
Authors: Wang Jian , Yu Yu-Die , Zhang Zhi-Guo , Wu Wei-Cheng , Sun Pei-Long , Cai Ming , Yang Kai
Published: September 2022

Starch nanoparticles (SNPs) were produced from sweet potato starches by ultrasonic treatment combined with rapid nanoprecipitation. The starch concentration, ultrasonic time, and the ratio of starch solution to ethanol were optimized through dynamic light scattering (DLS) technique to obtain SNPs with a Z-average size of 64.51 ± 0.15 nm, poly dispersity index (PDI) of 0.23 ± 0.01. However, after freeze drying, the SNPs showed varying degrees of aggregation depending on the particle size of SNPs before freeze-drying. The smaller the particle size, the more serious the aggregation. Therefore, we tried to treat SNPs with dielectric barrier discharge cold plasma before freeze drying. Properties including morphological features, crystalline structure and apparent viscosity of various starches were measured by field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), and rheometer, respectively. The results showed that, after cold plasma (CP) treatment, the aggregation of SNPs during freeze drying was significantly inhibited. Compared to the native sweet potato starch, SNPs showed a higher relative crystallinity and a lower apparent viscosity. After CP treatment, the relative crystallinity of CP SNPs was further higher, and the apparent viscosity was lower. This work provides new ideas for the preparation of SNPs and could promote the development of sweet potato SNPs in the field of active ingredient delivery.
A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems
Authors: Nagappa Lakshmeesha K. , Sato Wakana , Alam Farzana , Chengan Kameshwari , Smales Christopher M. , Von Der Haar Tobias , Polizzi Karen M. , Adamala Katarzyna P. , Moore Simon J.
Published: September 2022

Cell-free gene expression (CFE) systems are an attractive tool for engineering within synthetic biology and for industrial production of high-value recombinant proteins. CFE reactions require a cell extract, energy system, amino acids, and DNA, to catalyse mRNA transcription and protein synthesis. To provide an amino acid source, CFE systems typically use a commercial standard, which is often proprietary. Herein we show that a range of common microbiology rich media (i.e., tryptone, peptone, yeast extract and casamino acids) unexpectedly provide an effective and low-cost amino acid source. We show that this approach is generalisable, by comparing batch variability and protein production in the following range of CFE systems: Escherichia coli (Rosetta™ 2 (DE3), BL21(DE3)), Streptomyces venezuelae and Pichia pastoris. In all CFE systems, we show equivalent or increased protein synthesis capacity upon replacement of the commercial amino acid source. In conclusion, we suggest rich microbiology media provides a new amino acid source for CFE systems with potential broad use in synthetic biology and industrial biotechnology applications.
Pathophysiological roles of integrins in gliomas from the perspective of glioma stem cells
Authors: Wang Maoyu , Shen Sen , Hou Feng , Yan Yaohua
Published: September 2022

Glioblastoma is the most common primary intracranial tumor and is also one of the most malignant central nervous system tumors. Its characteristics, such as high malignancy, abundant tumor vasculature, drug resistance, and recurrence-prone nature, cause great suffering to glioma patients. Furthermore, glioma stem cells are the primordial cells of the glioma and play a central role in the development of glioma. Integrins—heterodimers composed of noncovalently bound a and ß subunits—are highly expressed in glioma stem cells and play an essential role in the self-renewal, differentiation, high drug resistance, and chemo-radiotherapy resistance of glioma stem cells through cell adhesion and signaling. However, there are various types of integrins, and their mechanisms of function on glioma stem cells are complex. Therefore, this article reviews the feasibility of treating gliomas by targeting integrins on glioma stem cells.