bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of social health insurance for hospital care by internal migrants in China—Evidence from the 2018 China migrants dynamic survey
Authors: Yao Qiang , Li Hanxuan , Liu Chaojie
Published: November 2022

Background

China's welfare system including social health insurance has been closely linked to its unique household registration system, despite high population mobility over the past few decades. This study aimed to determine the pattern of health insurance usage from internal migrants in mainland China for hospital care.

Methods

Data were extracted from the 2018 China Migrants Dynamic Survey. The respondents who enrolled in a social health insurance program and reported illness or injury over the past year were eligible for this study (n = 15,302). Two groups of outcome indicators were calculated assessing the use (incidence and settlement location) of insurance funds for hospital care and the burden of hospital expenditure (total hospital expenditure, out-of-pocket payments, and share of insurance reimbursement), respectively. Logit regression and Heckman's sample selection models were established to determine the predictors of insurance fund usage and the burden of hospital expenditure, respectively.

Results

Most respondents enrolled in a social health insurance program outside of their residential location (70.72%). About 28.90% were admitted to a hospital over the past year. Of those hospitalized, 72.98% were admitted to a hospital at their migration destination, and 69.96% obtained reimbursement from health insurance, covering on average 47% of total hospital expenditure. Those who had a local insurance fund aligned with residency (AOR = 2.642, 95% CI = 2.108–3.310, p < 0.001) and enrolled in employment-based insurance (AOR = 1.761, 95% CI = 1.348–2.301, p < 0.001) were more likely to use insurance funds for hospital care, and paid less out-of-pocket (β = −0.183 for local funds, p = 0.017; β = −0.171 for employment-based insurance, p = 0.005) than others. A higher share of insurance reimbursement as a proportion of hospital expenditure was found in the employment-based insurance enrollees (β = 0.147, p < 0.001). Insurance claim settlement at the residential location was associated with lower total hospital expenditure (β = −0.126, p = 0.012) and out-of-pocket payments (β = −0.262, p < 0.001), and higher share of insurance reimbursement (β = 0.066, p < 0.001) for hospital expenditure.

Conclusion

Low levels of health insurance benefits for hospital care are evident for internal migrants in mainland China, which are associated with the funding arrangements linked to household registration and inequality across different funds.
Street centrality and vitality of a healthy catering industry: A case study of Jinan, China
Authors: Chen Yanbin , Yin Guanwen , Hou Yiming
Published: November 2022

In the context of an overall improvement in the national economy, residents' demand for nutrition and health has been increasing. An industry that provides healthy eating plays an increasingly important role in urban development. Few studies, however, have focused on the relationship between the urban road network structure and the vitality of the healthy catering industry (VHCI). Based on social media data and urban traffic network data, in this study, we explored the impact mechanism of street centrality on the VHCI through a case study of Jinan, China, using geographic detectors and multiscale geographically weighted regression (MGWR) methods. The results show the following: First, the vitality of the catering industry in the main urban area of Jinan has multicore spatial distribution characteristics, and the high-value areas of the vitality of the catering industry are highly matched with the main business districts in the city in space. Second, we found clear spatial differences in street centrality between the walking and driving modes. With an increase in the search radius, the trend of high-value areas closely gathering in the urban center became clearer. The distribution of betweenness was observed from sheet to grid, and the multicenter structure of straightness was more prominent. Third, differences in the residents' perception of the road network structure caused by different travel modes affected their choice of dining places. In the driving mode, betweenness and straightness had a greater impact on the vitality of the catering industry, and the effect of closeness in the walking mode was more obvious. Fourth, the influence of street centrality on the vitality of the healthy catering industry had obvious spatial heterogeneity. In the walking mode, the spatial heterogeneity of straightness was the strongest, followed by betweenness, and closeness was the weakest; in the driving mode, the spatial heterogeneity of closeness was the strongest, followed by straightness, and betweenness was the weakest. From the perspective of residents' travel, the results of this study revealed the influence mechanism of urban road network characteristics on the VHCI. This information can aid planning for urban space optimization and improve residential living quality.
As the SARS-CoV-2 virus evolves, should Omicron subvariant BA.2 be subjected to quarantine, or should we learn to live with it?
Authors: Xu Ren , Wang Wanning , Zhang Wenlong
Published: November 2022

It has been nearly 35 months since the COVID-19 outbreak. The pathogen SARS-CoV-2 has evolved into several variants. Among them, Omicron is the fifth variant of concern which have rapidly spread globally during the past 8 months. Omicron variant shows different characteristics from previous variants, which is highly infectious, highly transmissible, minimally pathogenic, vaccine and antibody tolerant; however, it is less likely to cause severe illness, resulting in fewer deaths. Omicron has evolved into five main lineages, including BA.1, BA.2, BA.3, BA.4, and BA.5. Before BA.5, Omicron BA.2 sublineage was the dominant strain all over the world for several months. The experience of prevention and treatment against BA.2 is worth studying and learning for overcoming other Omicron subvariants. Although the Omicron subvariant BA.2 is significantly less severe than that caused by ancestral strains, it is still far more dangerous than influenza, and its long-term sequelae are unknown. Effective treatments are currently limited; therefore, effective defense may be the key to controlling the epidemic today, rather than just “living with” the virus.
Using the ISM-ANP-SD combination model to explore the mechanism and intervention strategies of influencing factors of coal mine safety system
Authors: Yang Xue , Xing Qiyu , Tian Kang , Liu Chen , Yang Juan
Published: November 2022

Background

With the intelligent construction of coal mines, the number of coal mine accidents is gradually decreasing, but the complexity of accidents is increasing. Understanding the interaction mechanism among the influencing factors of the coal mine safety system is an essential part of improving and enhancing the safety of the coal mine system.

Methods

The descriptive, structural model-network hierarchical analysis (ISM-ANP) was used to explore the interaction between the factors influencing the coal mine safety system and determine each factor's importance. A system dynamics simulation model was constructed to clarify the mechanism of each factor's effect on the safety system.

Results

The results show that Individual miners' factors directly influence coal mine system safety, organizational management factors, and group factors indirectly influence system safety and play the role of macro regulation. The intelligent system is the most profound factor influencing system safety. There are apparent differences in the influence of different subsystems on system safety, with organizational management having the most significant influence on system safety, followed by individual miners and group factors, and intelligent system factors and external environmental factors having a more negligible influence on system safety.

Conclusion

There is a complex interaction between the factors affecting the safety of the coal mine system, and there are apparent differences in the influence of different subsystems on the safety level of the coal mine system. This study puts forward the intervention strategy to improve the safety of the coal mine system, which provides theoretical support and method guidance for preventing coal mine accidents and improving the safety level of the coal mine system.
Utilization and out-of-pocket expenses of primary care among the multimorbid elderly in China: A two-part model with nationally representative data
Authors: Chen Yuehua , Liu Wenbin
Published: November 2022

Background

Multimorbidity has become an essential public health issue that threatens human health and leads to an increased disease burden. Primary care is the prevention and management of multimorbidity by providing continuous, comprehensive patient-centered services. Therefore, the study aimed to investigate the determinants of primary care utilization and out-of-pocket expenses (OOPE) among multimorbid elderly to promote rational utilization of primary care and reduce avoidable economic burdens.

Methods

The study used data from CHARLS 2015 and 2018, which included a total of 4,384 multimorbid elderly aged 60 and above. Guided by Grossman theory, determinants such as education, gender, marriage, household economy, and so on were included in this study. A two-part model was applied to evaluate primary care utilization and OOPE intensity in multimorbid populations. And the robustness testing was performed to verify research results.

Results

Primary care visits rate and OOPE indicated a decline from 2015 to 2018. Concerning primary outpatient care, the elderly who were female (OR = 1.51, P < 0.001), married (OR = 1.24, P < 0.05), living in rural areas (OR = 1.77, P < 0.001) and with poor self-rated health (OR = 2.23, P < 0.001) had a significantly higher probability of outpatient utilization, whereas those with middle school education (OR = 0.61, P < 0.001) and better household economy (OR = 0.96, P < 0.001) had a significantly less likelihood of using outpatient care. Rural patients (β = −0.72, P < 0.05) may have lower OOPE, while those with better household economy (β = 0.29, P < 0.05; β = 0.58, P < 0.05) and poor self-rated health (β = 0.62, P < 0.001) occurred higher OOPE. Regarding primary inpatient care, adults who were living in rural areas (OR = 1.48, P < 0.001), covered by Urban Employee Basic Medical Insurance (UEBMI) or Urban Rural Basic Medical Insurance (URBMI) (OR = 2.46, P < 0.001; OR = 1.81, P < 0.001) and with poor self-rated health (OR = 2.30, P < 0.001) had a significantly higher probability of using inpatient care, whereas individuals who were female (OR = 0.74, P < 0.001), with middle school education (OR = 0.40, P < 0.001) and better household economy (OR = 0.04, P < 0.001) had a significantly lower tendency to use inpatient care. Significantly, more OOPE occurred by individuals who were women (β = 0.18, P < 0.05) and with better household economy (β = 0.40, P < 0.001; β = 0.62, P < 0.001), whereas those who were covered by URBMI (β = −0.25, P < 0.05) and satisfied with their health (β = −0.21, P < 0.05) had less OOPE.

Conclusion

To prompt primary care visits and reduce economic burden among subgroups, more policy support is in need, such as tilting professional medical staff and funding to rural areas, enhancing awareness of disease prevention among vulnerable groups and so on.
Non-linear association of birth weight with lung function and risk of asthma: A population-based study
Authors: Yang Meng , Mei Hong , Du Juan , Yu Linling , Hu Liqin , Xiao Han
Published: November 2022

Background

The impact of birth weight on lung function and risk of asthma remains contentious. Our aim was to investigate the specific association of birth weight with lung function and the risk of asthma in children.

Methods

We performed cross-sectional analyses of 3,295 children aged 6–15 years who participated in the 2007–2012 National Health and Nutrition Examination Survey (NHANES). After controlling for potential covariates other than gestational diabetes, maternal asthma and obesity, the linear and non-linear associations of birth weight with lung function metrics and the risk of asthma were evaluated by a generalized linear model and generalized additive model, respectively.

Results

We observed a non-linear association of birth weight with FEV1 %predicted, FEV1/FVC %predicted and FEF25 − 75 %predicted (P for non-linearity was 0.0069, 0.0057, and 0.0027, respectively). Further threshold effect analysis of birth weight on lung function detected the turning point for birth weight was 3.6 kg. When the birth weight was < 3.6 kg, birth weight was significantly positively associated with all pulmonary function metrics. However, negative associations were found in FEV1 %predicted, FEV1/FVC %predicted and FEF25 − 75 %predicted when the birth weight was ≥3.6 kg. These results were consistent in the stratified and sensitivity analyses. Additionally, a possible non-linear relationship was also detected between birth weight and the risk of asthma.

Conclusion

Although not all maternal factors were accounted for, our findings provided new insight into the association of birth weight with lung function. Future studies are warranted to confirm the present findings and understand the clinical significance.
Water and climate: Global environmental sustainability and the current state in a developing country, Nicaragua
Authors: Vammen Katherine , Peña Elizabet
Published: November 2022

Environmental sustainability means taking steps to secure adequate management of natural resources in all human productive and livelihood activities. These steps involve a strategy of rational sustainable exploitation and the introduction of effective conservation measures which means integral management on all levels, national, regional and global. The specific situation of Nicaragua, as a prime example of a country in development with a vast richness in natural resources is analyzed. To name a few, in total renewable water resources the country has the 14th highest total yearly internal renewable water resources per capita (24,161/capita/year) in the Americas and as a tropical country has a large variety of biodiversity and ecosystems such as tropical dry and humid forests and 910 km of coastal area in the Caribbean and Pacific coast. These natural resources and more could bring potential to promote its own development. In spite of this, the country is on an unsustainable path as many parts of the world but with even greater challenges due to limited economic sustainability, high vulnerability to climate change and the lack of institutional capacity to establish better management. Unsustainable practices ranging from over-exploitation of resources to drastic land use changes have created environmental problems which consequently affect human wellbeing and health. Additionally, ecosystems and the quality of resources are under pressure which lead to a reduction in quality of water, forests and biodiversity. For example, the dry tropical forests have been reduced in 90% from 2000 to 2011 in the Pacific and Central areas and humid tropical forests are constantly being invaded and converted to agricultural and pasture lands. Water quality has been affected from contamination from agriculture, industries and the natural volcanic geology (arsenic) in groundwater as well as eutrophication and increased sedimentation from the watersheds of both lakes and rivers. Climate change is exacerbating these problems and causing new ones. After illustrating the present state of resources in Nicaragua, strategic suggestions of needed components to introduce an integral and improved management of the water resources have been laid out as a proposal for the future that include improvement in institutional capacity and governance to promote better management.
Additive-induced pH determines bacterial community composition and metabolome in traditional mustard seed fermented products
Authors: Bora Sudipta Sankar , Dullah Samim , Dey Kuntal Kumar , Hazarika Dibya Jyoti , Sarmah Unmona , Sharma Darshana , Goswami Gunajit , Singh Naorem Romen , Barooah Madhumita
Published: November 2022

Introduction

Kahudi and Kharoli are unique naturally fermented mustard seed products prepared and consumed in the northeastern region of India. The pre-fermentation processing of mustard seeds (soaking, pan-frying, mixing with alkaline or acidic additives, airtight packaging) renders a stringent fermentation environment. The metabolic activities of fermenting bacterial populations yield a myriad of glucosinolate-derived bioactive components which have not been described earlier.

Methods

This present study employed integrated 16S rRNA amplicon sequencing and LC-MS-based metabolomics to elucidate the bacterial diversity and metabolome of the two fermented mustard seed food products.

Results and Discussion

Univariate and multivariate analyses of metabolomics data revealed differential abundances of a few therapeutically-important metabolites viz., sinapine, indole-3-carbinol, γ-linolenic acid in Kahudi, and metabolites viz., β-sitosterol acetate, 3-butylene glucosinolate, erucic acid in Kharoli. A metagenomic investigation involving the 16S rRNA (V3–V4) amplicon sequencing showed the dominance of Firmicutes (99.1 ± 0.18%) in Kahudi, and Firmicutes (79.6 ± 1.92%) and Proteobacteria (20.37 ± 1.94%) in Kharoli. The most abundant genera were Bacillus (88.7 ± 1.67% in Kahudi; 12.5 ± 1.75% in Kharoli) followed by Lysinibacillus (67.1 ± 2.37% in Kharoli; 10.4 ± 1.74% in Kahudi). Members of both these genera are well known for proteolytic and endospore-forming abilities which could have helped in colonizing and thriving in the stringent fermentation environments.
Fitness of calves born from in vitro-produced fresh and cryopreserved embryos
Authors: Gómez Enrique , Murillo Antonio , Carrocera Susana , Pérez-Jánez Juan José , Benedito Jose Luis , Martín-González David , Gimeno Isabel
Published: November 2022

In cattle, vitrified/warmed (V/W) and frozen/thawed (F/T),in vitro-produced (IVP) embryos, differ in their physiology and survival from fresh embryos. In this study, we analyzed the effects of embryo cryopreservation techniques on the offspring. IVP embryos cultured with albumin and with or without 0.1% serum until Day 6, and thereafter in single culture without protein, were transferred to recipients on Day 7 as F/T, V/W, or fresh, resulting inN= 24, 14, and 13 calves, respectively. Calves were clinically examined at birth, and blood was analyzed before and after colostrum intake (Day 0), and subsequently on Day 15 and Day 30. On Day 0, calves from V/W and F/T embryos showed increased creatinine and capillary refill time (CRT) and reduced heartbeats. Calves from F/T embryos showed lower PCO2, hemoglobin, and packed cell volume than calves from V/W embryos while V/W embryos led to calves with increased Na+levels. Colostrum effects did not differ between calves from fresh and cryopreserved embryos, indicating similar adaptive ability among calves. However, PCO2did not decrease in calves from V/W embryos after colostrum intake. Serum in culture led to calves with affected (P< 0.05) temperature, CRT, HCO3-, base excess (BE), TCO2, creatinine, urea, and anion gap. On Day 15, the effects of embryo cryopreservation disappeared among calves. In contrast, Day 30 values were influenced by diarrhea appearance, mainly in calves from V/W embryos (i.e., lower values of TCO2, HCO3-, and BE; and increased glucose, anion gap, and lactate), although with no more clinical compromise than calves from fresh and F/T embryos. Diarrhea affected PCO2and Na+in all groups. Embryo cryopreservation, and/or culture, yield metabolically different calves, including effects on protein and acid–base metabolism.
How does imported pork regulate the supply and demand of China's pig market during the epidemic?—based on the analysis of African swine fever and COVID-19
Authors: Wang Jingjing , Wang Gangyi , Cui Yiniu , Zhang Jie
Published: November 2022

The pig industry is primarily a domestic industry in China is focused on ensuring the domestic pork supply. This paper analyzed changes in Chinese pork imports following the outbreaks of African Swine Fever (ASF) and COVID-19 between January 2017 to November 2020 and evaluated the impact of imported pork on the development of the swine industry in China. The results demonstrated that the shortage of domestic pork supply changed the import volume. ASF transformed imported pork from a complementary product to meet the diversified needs of domestic consumers into a critical substitute required to fill the supply gap. Following the COVID-19 outbreak, the substitution effect of imported pork decreased. ASF, has caused the supply capacity of pork in China to decrease, the price of pork to increase, leading to increased pork import in January 2019. At the end of 2019, pig slaughter decreased, while China cut tariffs on imported pork. The COVID-19 outbreak did not reduce China's pork imports in China, which declined after the global COVID-19 outbreak. Imported pork has made up for the supply gap during COVID-19, not impacting the level of production of the swine industry in China.
Advance in strategies to build efficient vaccines against tuberculosis
Authors: Qu Wei , Guo Yinhui , Xu Yan , Zhang Jie , Wang Zongchao , Ding Chaoyue , Pan Yuanhu
Published: November 2022

Tuberculosis is a chronic consumptive infectious disease, which can cause great damage to human and animal health all over the world. The emergence of multi-drug resistant strains, the unstable protective effect of Bacillus Calmette-Guérin (BCG) vaccine on adults, and the mixed infection with HIV all warn people to exploit new approaches for conquering tuberculosis. At present, there has been significant progress in developing tuberculosis vaccines, such as improved BCG vaccine, subunit vaccine, DNA vaccine, live attenuated vaccine and inactivated vaccine. Among these candidate vaccines, there are some promising vaccines to improve or replace BCG vaccine effect. Meanwhile, the application of adjuvants, prime-boost strategy, immunoinformatic tools and targeting components have been studied concentratedly, and verified as valid means of raising the efficiency of tuberculosis vaccines as well. In this paper, the latest advance in tuberculosis vaccines in recent years is reviewed to provide reliable information for future tuberculosis prevention and treatment.
Association of Household Utility of Cleaner Fuel With Lower Hypertension Prevalence and Blood Pressure in Chinese Adults
Authors: Liu Zhiguang , Li Mengya , Zhu Yibing , Hystad Perry , Ma Yuanting , Rangarajan Sumathy , Zhao Qian , Hu Lihua , Yusuf Salim , Li Yang , Tse Lap Ah
Published: November 2022

Objectives: To investigate whether lower hypertension prevalence or blood pressure was associated with cleaner household fuel usage for cooking and heating among Chinese adults.

Methods: We enrolled 44,862 Chinese adults at the baseline of the prospective urban and rural epidemiology (PURE) study in China during 2005–2009, as a subset of the PURE-global China site. Multilevel logistic regression and generalized linear mixed models were conducted to estimate the adjusted odds ratio (AOR) and regression coefficient for hypertension and blood pressure respectively, while subgroup analysis by ambient PM2.5 concentration and location was also examined.

Results: Compared with the least clean household solid fuel group, gas (AOR = 0.91, 95% CI: 0.83, 0.99) or electricity (AOR = 0.72, 95% CI: 0.60, 0.87) was associated with significantly lower levels of hypertension prevalence and blood pressure, and a similar pattern of the association was consistently observed among participants with high ambient PM2.5 exposure and those living in urban areas.

Conclusion: Household utility of cleaner fuel type was associated with lower hypertension prevalence and blood pressure in Chinese adults. Our study urges the utilization of cleaner household energy to mitigate the burden of hypertension.
EU Dairy after the Quota Abolition: Inelastic Asymmetric Price Responsiveness and Adverse Milk Supply during Crisis Time
Authors: Jongeneel Roel , Gonzalez-Martinez Ana
Published: November 2022

The abolition of the milk quota system in April 2015 has implied a transition towards a new “system” in which non-policy and non-EU elements have become important drivers of the EU dairy market. In terms of its contribution, after reviewing some of the existing literature, this article presents a theoretical framework to understand “irregular” supply behaviour, while it empirically identifies an “inverse” supply curve for the EU dairy sector. In doing so, milk prices have been decomposed by following the procedure proposed by James P. Houck. This exercise confirms the non-reversibility, and therefore an asymmetric response, in the behaviour of milk supply for most EU Member States. Moreover, this article also presents the results of a “stress” test of the dairy sector in order to analyse the responses of dairy production in a context of the asymmetrical behaviour of dairy farmers and assess the competitiveness of the dairy sector at Member State level. The outcomes emphasise the high competitiveness of the Irish dairy sector and suggest a deterioration of the competitiveness in the case of the United Kingdom and France. From the policy-making perspective, the identification of this type of farmer’s reactions is key for an appropriate design of policy interventions and crisis management strategies.
Tanzanian Farmers’ Intention to Adopt Improved Maize Technology: Analyzing Influencing Factors Using SEM and fsQCA Methods
Authors: Jin Ye , Lin Qingning , Mao Shiping
Published: November 2022

The China–Tanzania Village-based Learning Center for Poverty Reduction project aims to demonstrate China’s experience in poverty reduction through developing smallholder agriculture at the village level, and through the promotion of improved technologies with the purpose of increasing agricultural productivity and improving village-level agricultural production. However, to promote technology application effectively, a better understanding of farmers’ behavioral intention toward improved maize technology is needed. This study uses microdata from 282 Tanzanian maize farmers. Compared with previous research, the innovation of our study is that the methods of structural equation model (SEM) and fuzzy-set qualitative comparative analysis (fsQCA) are applied to study the influencing factors of farmers’ intention to adopt improved maize technology and the combination paths that affect farmers’ intention. The analysis results show that farmers’ behavior perceptions and compatibility perceptions positively impact their intention to choose agricultural technology. Three modes can trigger farmers’ intention to adopt technology: “behavior perception, compatibility perception, non-self-efficacy, facilitation conditions,” “behavior perception, social impact, non-self-efficacy, facilitation conditions,” and “compatibility perception, social impact, self-efficacy, facilitation conditions.” To increase maize yield and promote the extension of improved agricultural technology through the China–Tanzania Village-based Learning Center for Poverty Reduction project, it is necessary to choose an effective combination path to influence farmers’ intention to adopt the proposed changes. If farmers’ intention to adopt improved maize technology can be increased to a greater extent, it can accelerate the improvement of agricultural technology in Tanzania, thereby increasing agricultural productivity, improving agricultural production at the village level, and reducing poverty.
Soil Carbon and Nitrogen Stocks under Agrosilvopastoral Systems with Different Arrangements in a Transition Area between Cerrado and Caatinga Biomes in Brazil
Authors: Freitas Igor Costa de , Alves Matheus Almeida , Magalhães Jaílson Ramos , Dias Rafael Ferreira , Albuquerque Carlos Juliano Brant , Ferreira Evander Alves , Fernandes Luiz Arnaldo , Pegoraro Rodinei Facco , Frazão Leidivan Almeida
Published: November 2022

Production systems that promote the accumulation of soil organic matter (SOM) must be implemented to maintain the sustainability of agriculture, livestock, and forestry. Since increases in MOS content contribute to improving the chemical, physical, and biological quality of the soil, as well as helping to reduce carbon emissions to mitigate climate change. Therefore, the objective of this study was to evaluate soil organic carbon (SOC) and nitrogen (N) stocks after the implementation of agrosilvopastoral (ASP) systems in a Cerrado-Caatinga transition zone in Brazil. Native vegetation of Cerrado-Caatinga (NV), regenerating stratum of Cerrado-Caatinga (RS), two arrangements of ASP systems cultivating Cenchrus ciliaris L. intercropped with Eucalyptus camaldulensis Dehnh. × Eucalyptus tereticornis Sm. hybrid (ASP1 and ASP2), and intercropped with Eucalyptus urophylla S.T. Blake × Eucalyptus grandis W. Mill ex Maiden hybrid (ASP3 and ASP4) were evaluated. Soil C and N stocks and the C content in the humic fractions of SOM were evaluated at 0–10, 10–20, and 20–30 cm soil depths. The introduction of ASP2, ASP3, and ASP4 systems in an area previously occupied by low productivity pasture increased and restored SOC stocks to levels found in NV, at a depth of 0–30 cm. N stocks were higher in ASP systems, regardless of the arrangement studied. As a result, the ASP systems provided accumulations that ranged from 1.0 to 4.31 Mg SOC ha−1 yr−1 and from 0.33 to 0.36 Mg N ha−1 yr−1. The carbon contents in humic fractions remained higher in NV. The hierarchical grouping and principal component analysis showed that the implementation of the ASP systems was efficient in increasing soil C and N stocks over time. In conclusion, the present study identified that integrated production systems can support land use intensification strategies based on sustainable and low-carbon agriculture in a transition area between the Cerrado and Caatinga biomes in Brazil.
The Effects of Nitrogen Fertilisation on Plant Species Richness in European Permanent Grasslands: A Systematic Review and Meta-Analysis
Authors: Francksen Richard M. , Turnbull Samantha , Rhymer Caroline M. , Hiron Matthew , Bufe Conny , Klaus Valentin H. , Newell-Price Paul , Stewart Gavin , Whittingham Mark J.
Published: November 2022

Nitrogen fertilisation is a common form of agricultural intensification, aimed at increasing biomass, which can affect plant species diversity and ecosystem functioning. Using a systematic review and meta-analysis of nitrogen fertilisation studies in European permanent grasslands, we asked: (i) what relationship form exists between nitrogen application rate and change in plant diversity, compared to zero fertilisation controls; and (ii) how grassland, management and study characteristics affect this relationship. Meta-analysis of 34 control-treatment effects from 14 studies conducted across nine European countries revealed a negative linear relationship between nitrogen fertilisation rate and change in plant species richness, equivalent to approximately 1.5 species/m2 lost for every 100 Kg ha−1 yr−1 of nitrogen added. Fertilisation induced reductions in plant species richness were greater when defoliation rates were lower. We found some evidence that grasslands with a higher baseline plant diversity lost more species when fertilised compared to more species poor grasslands, although uncertainty was high. Due to the diverse grassland types included in the analysis, the variability in fertilisation-driven changes in plant diversity was high. We identified several remaining limitations to our understanding, including uncertainty about non-linear effects, which could aid efforts to optimise the trade-off of plant diversity and increasing grassland yields.
Highly Dispersed Blast-Furnace Sludge as a New Micronutrient Fertilizer: Promising Results on Rapeseed
Authors: Zakharova Olga V. , Baranchikov Peter A. , Grodetskaya Tatiana A. , Kuznetsov Denis V. , Gusev Alexander A.
Published: November 2022

Due to the growing population of Earth, the problem of providing food comes to the fore. Therefore, the search for new, economically available sources of trace elements for crop production is relevant. One of these potential sources is blast-furnace sludge: highly dispersed metallurgical waste, the industrial processing of which is difficult due to its high zinc content. We studied the effect of blast-furnace sludge on rapeseed plants in laboratory, greenhouse, and field experiments and also assessed the accumulation of sludge components in plant organs. The studied sludge sample consisted of micron and submicron particles containing compounds of iron, silicon, aluminum, zinc, calcium, and sulfur. Used concentrations: laboratory—0.01, 0.1, 1%, 10, and 100 g L−1; greenhouse—0.01, 0.1, 1, 10, and 100 g kg−1; field—0.5, 2, and 4 t ha−1. During a laboratory experiment, a decrease in the germination of rapeseed seeds exposed to 0.01, 0.1, 10, and 100 g L−1 waste was observed, but 1 g L−1 promoted the increase of this indicator by 7% regarding control (0 g L−1). While inhibiting seed germination, the sludge had a beneficial effect on the vegetative performance of plants. Reverse effects were noted in the greenhouse experiment as an increase in seed germination (introduction of 1 g kg−1 of sludge to the substrate caused maximum stimulation) and a decrease in rapeseed morphometric parameters were observed. However, at a concentration of 10 g kg−1, the root mass increased by 43% and the stem mass by 63%. In the same group, the highest content of chlorophylls was noted. The number of pods in all experimental groups of plants was less than in control (0 g kg−1) plants, but at the same time, in the variants of 0.01 and 1 g kg−1, the weight of seeds was noticeably increased, by 15.6 and 50%, respectively. Under the conditions of the field experiment, the sludge had a positive effect on the indicators of biological and economic productivity. Thus, exposure to 0.5 and 2 t ha−1 of sludge significantly increased the dry matter and leaf area. The highest values of photosynthetic capacity were recorded at a dose of 2 t ha−1. The maximum increase in yield was ensured by the introduction of sludge at a concentration of 0.5 and 2 t ha−1. The sludge dose of 4 t ha−1, which was also used, either had no effect or suppressed the development of the analyzed traits. The study of the accumulation of zinc and iron in the organs of plants showed the absence of a pronounced dose-dependent accumulation of zinc in the organs of rapeseed, while for iron, an increase in the content of the element in the organs of plants associated with an increase in the concentration of sludge in the soil was recorded. Our results demonstrate the promise of further research and development of methods for the agricultural use of highly dispersed sludge from wet gas cleaning of blast furnace production.
Recycling Phosphorus from Agricultural Streams: Grey and Green Solutions
Authors: Auteri Nicolò , Saiano Filippo , Scalenghe Riccardo
Published: November 2022

Many intensively farmed soils show high phosphorus (P) contents compared to the thresholds required for agricultural production; 0.084 Mt of P year−1 is leaving the European terrestrial system. This paper focuses mainly on non-point flows of P and provides an overview of the most promising and sustainable solutions for P recycling, centred on waste materials from agriculture. Given the global shortage of the primary resource of P, its management is critical for its efficient use. Nowadays, wastage and loss at every stage of the P cycle raise concerns about future supplies and especially about the resulting environmental problems, such as the eutrophication of surface water bodies and the reduction of biodiversity. Recovering P costs more than EUR 640 per tonne depending on the type of technique used. The opportunity for P recovery with green and sustainable technology is, therefore, a great challenge for the next years. Waste materials or by-products of agricultural processing have been considered ecologically safe, low-cost, and highly selective with high pollutant adsorption capacities, which would enable sustainable P recovery, both environmentally and economically. A realistic threshold for considering the reuse of P sustainably at the farm level is EUR 320 per tonne.
Effects of Antioxidant Gene Overexpression on Stress Resistance and Malignization In Vitro and In Vivo: A Review
Authors: Tavleeva Marina M. , Belykh Elena S. , Rybak Anna V. , Rasova Elena E. , Chernykh Aleksey A. , Ismailov Zaur B. , Velegzhaninov Ilya O.
Published: November 2022

Reactive oxygen species (ROS) are normal products of a number of biochemical reactions and are important signaling molecules. However, at the same time, they are toxic to cells and have to be strictly regulated by their antioxidant systems. The etiology and pathogenesis of many diseases are associated with increased ROS levels, and many external stress factors directly or indirectly cause oxidative stress in cells. Within this context, the overexpression of genes encoding the proteins in antioxidant systems seems to have become a viable approach to decrease the oxidative stress caused by pathological conditions and to increase cellular stress resistance. However, such manipulations unavoidably lead to side effects, the most dangerous of which is an increased probability of healthy tissue malignization or increased tumor aggression. The aims of the present review were to collect and systematize the results of studies devoted to the effects resulting from the overexpression of antioxidant system genes on stress resistance and carcinogenesis in vitro and in vivo. In most cases, the overexpression of these genes was shown to increase cell and organism resistances to factors that induce oxidative and genotoxic stress but to also have different effects on cancer initiation and promotion. The last fact greatly limits perspectives of such manipulations in practice. The overexpression of GPX3 and SOD3 encoding secreted proteins seems to be the “safest” among the genes that can increase cell resistance to oxidative stress. High efficiency and safety potential can also be found for SOD2 overexpression in combinations with GPX1 or CAT and for similar combinations that lead to no significant changes in H2O2 levels. Accumulation, systematization, and the integral analysis of data on antioxidant gene overexpression effects can help to develop approaches for practical uses in biomedical and agricultural areas. Additionally, a number of factors such as genetic and functional context, cell and tissue type, differences in the function of transcripts of one and the same gene, regulatory interactions, and additional functions should be taken into account.
Correction: Stanciauskaite et al. Balsam Poplar Buds: Extraction of Potential Phenolic Compounds with Polyethylene Glycol Aqueous Solution, Thermal Sterilization of Extracts and Challenges to Their Application in Topical Ocular Formulations. Antioxidants 2022, 11, 1771
Authors: Stanciauskaite Monika , Marksa Mindaugas , Ivanauskas Liudas , Ramanauskiene Kristina
Published: November 2022

In the original publication [...]
Astaxanthin Protects against Hyperglycemia-Induced Oxidative and Inflammatory Damage to Bone Marrow and to Bone Marrow-Retained Stem Cells and Restores Normal Hematopoiesis in Streptozotocin-Induced Diabetic Mice
Authors: Bhattarai Govinda , So Han-Sol , Kim Tae-Geum , Kieu Thi Thu Trang , Kim Yeon-Woo , Yang Ku-Ri , Lee Jeong-Chae , Kook Sung-Ho , Jeon Young-Mi
Published: November 2022

Hyperglycemia has various adverse health effects, some of which are due to chronic oxidative and inflammatory impairment of bone marrow (BM), hematopoietic stem cells (HSCs), and mesenchymal stem cells (MSCs). Astaxanthin (ASTX) has been shown to ameliorate hyperglycemia-associated systemic complications and acute mortality, and this effect is partially associated with restoration of normal hematopoiesis. Here, the effects of ASTX on diabetes-induced complications in BM and BM stem cells were investigated, and the underlying molecular mechanisms were elucidated. Ten-week-old C57BL/6 mice received a single intraperitoneal injection of streptozotocin (STZ; 150 mg/kg) in combination with oral gavage of ASTX (12.5 mg/kg) for 30 or 60 consecutive days. Supplemental ASTX ameliorated acute mortality and restored the STZ-impaired bone mass accrual and BM microenvironment in STZ-injected mice. Oral gavage of ASTX suppressed osteoclast formation in the BM of STZ-injected mice. Specifically, supplementation with ASTX inhibited oxidative stress and senescence induction of BM HSCs and MSCs and ameliorated hematopoietic disorders in STZ-injected mice. These effects of ASTX were associated with BM restoration of angiopoietin 1, stromal cell-derived factor 1, β-catenin, and Nrf2. Long-term ASTX gavage also recovered the STZ-induced dysfunction in migration, colony formation, and mineralization of BM-derived stromal cells. Further, a direct addition of ASTX exhibited direct and dose-dependent inhibition of osteoclastic activation without cytotoxic effects. Collectively, these results indicate that ASTX protects against diabetes-induced damage in the BM microenvironment in BM, HSCs, and MSCs and restores normal hematopoiesis and bone accrual in STZ-injected mice.
Mycosynthesis of Silica Nanoparticles Using Aspergillus niger: Control of Alternaria solani Causing Early Blight Disease, Induction of Innate Immunity and Reducing of Oxidative Stress in Eggplant
Authors: Albalawi Marzough A. , Abdelaziz Amer M. , Attia Mohamed S. , Saied Ebrahim , Elganzory Hussein H. , Hashem Amr H.
Published: November 2022

The threats to the life and production of crops are exacerbated by climate change and the misuse of chemical pesticides. This study was designed to evaluate the effectiveness of biosynthesized silica nanoparticles (SiO2-NPs) as an alternative to pesticides against early blight disease of eggplant. Antifungal activity, disease index, photosynthetic pigments, osmolytes, oxidative stress, antioxidant enzymes activities were tested for potential tolerance of eggplant infected with Alternaria solani. Silica nanoparticles were successfully biosynthesized using Aspergillus niger through green and ecofriendly method. Results revealed that SiO2-NPs exhibited promising antifungal activity against A. solani where MIC was 62.5 µg/mL, and inhibition growth at concentration 1000 µg/mL recorded 87.8%. The disease Index (DI) as a result of infection with A. solani reached 82.5%, and as a result, a severe decrease in stem and root length and number of leaves occurred, which led to a sharp decrease in the photosynthetic pigments. However, contents of free proline, total phenol and antioxidant enzymes activity were increased in infected plants. On the other hand, the treatment with SiO2-NPs 100 ppm led to a great reduction in the disease Index (DI) by 25% and a high protection rate by 69.69%. A clear improvement in growth characteristics and a high content of chlorophyll and total carotenoids was also observed in the plants as a result of treatment with silica nanoparticles in (healthy and infected) plants. Interestingly, the noticeable rise in the content of infected and healthy plants of proline and phenols and an increase in the activity of super oxide dismutase (SOD) and polyphenol oxidase (PPO). It could be suggested that foliar application of SiO2-NPs especially 100 ppm could be commercially used as antifungal and strong inducer of plant physiological immunity against early blight disease.
Relationship of Thermal Treatment and Antioxidant Capacity in Cooked Foods
Authors: Navajas-Porras Beatriz , Pérez-Burillo Sergio , Hinojosa-Nogueira Daniel , Pastoriza Silvia , Rufián-Henares José Ángel
Published: November 2022

Most of the foods we eat undergo a cooking process before they are eaten. During such a process, the non-enzymatic browning occurs, which generates compounds such as furosine, 5-hydroxymethylfurfural (HMF) and furfural. These are considered markers of cookedness and can therefore be used as quality indicators. In this work, we study the production of these compounds in different foods (both of plant and animal origin) that are cooked with different techniques. Additionally, we investigate correlations between the production of these markers of cookedness and the antioxidant capacity produced after in vitro digestion and fermentation. We observe that, in general, cereals and vegetables are more thermally damaged. Toasting and frying produce the highest concentrations of Maillard compounds whereas boiling the lowest. Furosine content shows a significant positive correlation with in vitro digestion data in fried foods, and with fermentation in roasted foods. Furfural content shows a significant positive correlation with in vitro digestion results in roasted foods, specifically in the Folin–Ciocalteu method.
A Benchmark for the Evaluation of Corner Detectors
Authors: Zhang Yang , Zhong Baojiang , Sun Xun
Published: November 2022

Corners are an important kind of image feature and play a crucial role in solving various tasks. Over the past few decades, a great number of corner detectors have been proposed. However, there is no benchmark dataset with labeled ground-truth corners and unified metrics to evaluate their corner detection performance. In this paper, we build three benchmark datasets for corner detection. The first two consist of those binary and gray-value images that have been commonly used in previous corner detection studies. The third one contains a set of urban images, called the Urban-Corner dataset. For each test image in these three datasets, the ground-truth corners are manually labeled as objectively as possible with the assistance of a line segment detector. Then, a set of benchmark evaluation metrics is suggested, including five conventional ones: the precision, the recall, the arithmetic mean of precision and recall (APR), the F score, the localization error (Le), and a new one proposed in this work called the repeatability referenced to ground truth (RGT). Finally, a comprehensive evaluation of current state-of-the-art corner detectors is conducted.
Effect of a Conical Cellulose Structure on Horseradish Peroxidase Biomacromolecules
Authors: Ivanov Yuri D. , Tatur Vadim Y. , Shumov Ivan D. , Kozlov Andrey F. , Valueva Anastasia A. , Ivanova Irina A. , Ershova Maria O. , Ivanova Nina D. , Stepanov Igor N. , Lukyanitsa Andrei A. , Ziborov Vadim S.
Published: November 2022

The effect of a dielectric conical structure on the adsorption properties of an enzyme on mica was studied by atomic force microscopy (AFM) with the example of horseradish peroxidase (HRP). The cone used was a cellulose cone with a 60° apex angle. Namely, AFM allowed us to reveal an increase in the enzyme’s aggregation during its adsorption onto mica from the solution incubated near the cone apex for 40 min—as compared with the control enzyme samples incubated far away from the cone. In contrast, no change in the HRP adsorption properties was observed after shorter (10 min) incubation of the sample near the cone. The enzymatic activity of HRP was found to be the same for all the enzyme samples studied. Our findings should be considered upon designing biosensors (in particular, those intended for highly sensitive diagnostic applications) and bioreactors containing conical structural elements. Furthermore, since HRP is widely employed as a model enzyme in studies of external impacts on enzymes determining food quality, our data can be of use in the development of food-processing methods based on the use of electromagnetic radiation (microwave treatment, radiofrequency heating, etc.).