bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibition of Serine Proteases as a Novel Therapeutic Strategy for Abdominal Pain in IBS
Authors: Decraecker Lisse , Boeckxstaens Guy , Denadai-Souza Alexandre
Published: May 2022

Serine proteases are heavily present in the gastrointestinal tract where they are essential in numerous physiological processes. An imbalance in the proteolytic activity is a central mechanism underlying abdominal pain in irritable bowel syndrome (IBS). Therefore, protease inhibitors are emerging as a promising therapeutic tool to manage abdominal pain in this functional gastrointestinal disorder. With this review, we provide an up-to-date overview of the implications of serine proteases in the development of abdominal pain in IBS, along with a critical assessment of the current developments and prospects of protease inhibitors as a therapeutic tool. In particular, we highlight the current knowledge gap concerning the identity of dysregulated serine proteases that are released by the rectal mucosa of IBS patients. Finally, we suggest a workflow with state-of-the-art techniques that will help address the knowledge gap, guiding future research towards the development of more effective and selective protease inhibitors to manage abdominal pain in IBS.
Variations in Plant Water Use Efficiency Response to Manipulated Precipitation in a Temperate Grassland
Authors: Hai Xuying , Li Jianping , Li Jiwei , Liu Yulin , Dong Lingbo , Wang Xiaozhen , Lv Wenwen , Hu Zhenhong , Shangguan Zhouping , Deng Lei
Published: May 2022

Water use efficiency (WUE) plays important role in understanding the interaction between carbon and water cycles in the plant-soil-atmosphere system. However, little is known regarding the impact of altered precipitation on plant WUE in arid and semi-arid regions. The study examined the effects of altered precipitation [i.e., ambient precipitation (100% of natural precipitation), decreased precipitation (DP, −50%) and increased precipitation (IP, +50%)] on the WUE of grass species (Stipa grandis and Stipa bungeana) and forb species (Artemisia gmelinii) in a temperate grassland. The results found that WUE was significantly affected by growth stages, precipitation and plant species. DP increased the WUE of S. grandis and S. bungeana generally, but IP decreased WUE especially in A. gmelinii. And the grasses had the higher WUE than forbs. For different growth stages, the WUE in the initial growth stage was lower than that in the middle and late growth stages. Soil temperature, available nutrients (i.e., NO3–, NH4+, and AP) and microorganisms under the altered precipitations were the main factors affecting plant WUE. These findings highlighted that the grasses have higher WUE than forbs, which can be given priority to vegetation restoration in arid and semi-arid areas.
Parental Genome Imbalance Causes Hybrid Seed Lethality as Well as Ovary Abscission in Interspecific and Interploidy Crosses in Nicotiana
Authors: He Hai , Sadahisa Kumi , Yokoi Shuji , Tezuka Takahiro
Published: May 2022

Enhanced ovary abscission after pollination and hybrid seed lethality result in post-zygotic reproductive isolation in plant interspecific crosses. However, the connection between these barriers remains unclear. Here, we report that an imbalance in parental genomes or endosperm balance number (EBN) causes hybrid seed lethality and ovary abscission in both interspecific and intraspecific-interploidy crosses in the genus Nicotiana. Auxin treatment suppressed ovary abscission, but not hybrid seed lethality, in an interspecific cross between Nicotiana suaveolens and N. tabacum, suggesting that ovary abscission-related genes are located downstream of those involved in hybrid seed lethality. We performed interploidy crosses among N. suaveolens tetraploids, octoploids, and neopolyploids and revealed hybrid seed lethality and ovary abscission in interploid crosses. Furthermore, a higher maternal EBN than paternal EBN caused these barriers, as previously observed in N. suaveolens × N. tabacum crosses. Altogether, these results suggest that maternal excess of EBN causes hybrid seed lethality, which in turn leads to ovary abscission through the same mechanism in both interspecific and interploidy crosses.
Exploring the Effects of Argument Map-Supported Online Group Debate Activities on College Students’ Critical Thinking
Authors: Chen Xinya , Wang Li , Zhai Xuesong , Li Yan
Published: May 2022

Debate has been warranted as a meaningful activity to promote students’ higher-level thinking, such as critical thinking. However, traditional debate activities which are typically carried out in the physical classroom may meet some obstructions of limited time and space, which would result in the phenomenon that many participants act as silent watchers rather than mind exchangers. Moreover, it is hard to make a visualized record about the whole process and contents of the traditional debate activity. The current study aimed to explore the effects of argument map (AM)-supported online group debate activities on college students’ critical thinking, including their depth and phases of critical thinking, and the relationship between students’ depth of critical thinking and their number of speeches. In the study, an innovative argumentation construction way was designed. All students enrolled in a course could have a chance to attend the AM-supported online group debate activities and the whole process and outcomes of online group debate could be visualized by AM. At the same time, the effectiveness of the innovative activities was evaluated by content analysis of AM. A total of 42 sophomores in the undergraduate course named “Learning Sciences and Technology” were recruited to attend online group debate activities assisted by a web-based visualization tool named “ZJU Yuque” in 5 weeks. Newman’s framework about the depth of critical thinking and Murthy’s instrument of critical thinking phases were employed as guidelines. We found that 42 students’ overall depth of critical thinking was gradually improved in an obvious way during the five online debate activities. The most frequent phases for students in the first and second team in online group debate activities include Understand→Understand (U→U), Recognize→Understand (R→U), and Understand→Evaluate (U→E). However, students’ creating behaviors were not significantly generated. Teachers’ real-time feedback was helpful for students’ improvements of high-level thinking skills and their preparation for the next debate activity. Students’ interviews found that students highly valued such online group debate activities because every student could have a chance to express their thoughts and they had enough time to prepare debate contents. Based on the findings, some implications were proposed for the better design and implementation of online group debate activities.
Developing and Validating the Teacher Rating Scale of Learning Interest for Kindergarteners
Authors: Wu Chung Chin
Published: May 2022

Studies have investigated learning interest based on either 2- or 4-factor theoretical framework. Empirical studies showed supportive evidence only toward the 2-factor learning interest model, but it was primarily demonstrated above the secondary level. It is unclear whether the dimensionality of the learning interest of kindergarteners is consistent with those studies conducted above the secondary level due to the absence of an instrument for measuring kindergarteners' learning interests. An effective and efficient learning interest scale was developed and validated for teachers' use to rate kindergarteners to provide useful information for improving teaching and learning in practice. A total of 132 5-year-old kindergarteners were rated by 5 teachers in the formal study. The results clearly showed: (1) the developed teacher rating scale of learning interest was valid for understanding kindergarteners' learning interests and was equally suitable for boys and girls. (2) The 2-factor learning interest model was the best theoretical viewpoint for understanding kindergarteners' learning interests across gender. The implications for learning interest research and practice are also discussed.
Development of a Novel Retina−Based Diagnostic Score for Early Detection of Major Depressive Disorder: An Interdisciplinary View
Authors: Liu Xiao , Lai Shunkai , Ma Shisi , Yang Hong , Liu Lian , Yu Guocheng , Zhong Shuming , Jia Yanbin , Zhong Jingxiang
Published: May 2022

Background

Clinically effective markers for the diagnosis of major depressive disorder (MDD) are lacking. Alterations in retinal features are closely related to the pathophysiological progression of MDD. However, the reliable retina-related diagnostic model for MDD remains to be developed. Thus, our study aimed to quantitatively evaluate retinal vascular and structural changes in MDD patients and to develop a reliable diagnostic model of MDD based on retinal parameters.

Methods

Seventy-eight patients with MDD and 47 healthy controls (HCs) underwent retinal vessel density and structure examination using optical coherence tomography angiography and visual field examination using perimetry. Independent-sample t test was used to assess the differences in retinal parameters between the groups. Meanwhile, we constructed the corresponding retina-based diagnostic model by LASSO logistic regression. Finally, the diagnostic ability of the model was evaluated by area under the curve (AUC) of receiver operating characteristic curves and calibration plot of nomogram.

Results

MDD patients showed lower retinal vessel density (including radial peripapillary capillary vessel density, superficial and deep capillary plexus vessel density), thinner subfoveal choroidal thickness, and poorer visual fields compared to HCs (all p < 0.05). Furthermore, a retina-based diagnostic model was constructed and shows a strong diagnostic capability for MDD (AUC = 0.9015, p < 0.001).

Conclusion

Patients with MDD showed distinct retinal features compared to HCs. The retina-based diagnostic model is expected to be a necessary complement to the diagnosis of MDD.
Economic Burden and Quality of Life of Hepatocellular Carcinoma in Greater China: A Systematic Review
Authors: Zou Huimin , Li Meng , Lei Qing , Luo Zejun , Xue Yan , Yao Dongning , Lai Yunfeng , Ung Carolina Oi Lam , Hu Hao
Published: May 2022

Background

Hepatocellular carcinoma (HCC) accounts for more than 85%-90% of primary liver cancer globally, and approximately 45% of deaths from HCC occur in greater China. This disease poses a significant economic burden for patients, payers and society and significantly affects patients' quality of life (QoL). However, such impact of HCC in greater China has not been well characterized. This review was conducted to analyze the current evidence about the economic and humanistic impact of HCC in greater China for informing national disease management and identifying clinical gaps yet to be resolved.

Methods

A systematic search literature using seven databases (Web of Science, PubMed, Medline, Cochrane Central, China National Knowledge Infrastructure, Wanfang, and Weipu) was performed to identify interventional and observational studies that reported the impact of HCC on cost or QoL and published before April 6, 2021. The focus population included adult patients with HCC in greater China. This review excluded any studies that focused on any specific treatment. Study quality was assessed using the Effective Public Health Practice Project tool.

Results

Of 39,930 studies retrieved, 27 were deemed eligible for inclusion. The methodologies, perspectives and data sources used in studies were heterogeneous. In greater China, while few studies reported the health expenditures of HCC patients and investigations about economic burden at national level was lacking, the significant economic impact of HCC on patients and their families had been reported. Health-related costs increased as the disease deteriorated. Additionally, HCC also has a negative impact on the QoL of patients, mostly in terms of physical, cognitive, social functioning and severe symptoms.

Conclusions

HCC has brought significant economic and QoL burden to patients in greater China. Both physical and psychological factors predicted QoL in patients with HCC in greater China. Future studies should explore the disease-related economic effects on Chinese patients and their families, the effects of physical and psychological factors on QoL and the relationships of physical and psychological factors in the region.

Systematic Review Registration:www.crd.york.ac.uk/prospero/display_record.php?RecordID=278421, PROSPERO: CRD42021278421
Towards Integrated Air Pollution Monitoring and Health Impact Assessment Using Federated Learning: A Systematic Review
Authors: Neo En Xin , Hasikin Khairunnisa , Mokhtar Mohd Istajib , Lai Khin Wee , Azizan Muhammad Mokhzaini , Razak Sarah Abdul , Hizaddin Hanee Farzana
Published: May 2022

Environmental issues such as environmental pollutions and climate change are the impacts of globalization and become debatable issues among academics and industry key players. One of the environmental issues which is air pollution has been catching attention among industrialists, researchers, and communities around the world. However, it has always neglected until the impacts on human health become worse, and at times, irreversible. Human exposure to air pollutant such as particulate matters, sulfur dioxide, ozone and carbon monoxide contributed to adverse health hazards which result in respiratory diseases, cardiorespiratory diseases, cancers, and worst, can lead to death. This has led to a spike increase of hospitalization and emergency department visits especially at areas with worse pollution cases that seriously impacting human life and health. To address this alarming issue, a predictive model of air pollution is crucial in assessing the impacts of health due to air pollution. It is also critical in predicting the air quality index when assessing the risk contributed by air pollutant exposure. Hence, this systemic review explores the existing studies on anticipating air quality impact to human health using the advancement of Artificial Intelligence (AI). From the extensive review, we highlighted research gaps in this field that are worth to inquire. Our study proposes to develop an AI-based integrated environmental and health impact assessment system using federated learning. This is specifically aims to identify the association of health impact and pollution based on socio-economic activities and predict the Air Quality Index (AQI) for impact assessment. The output of the system will be utilized for hospitals and healthcare services management and planning. The proposed solution is expected to accommodate the needs of the critical and prioritization of sensitive group of publics during pollution seasons. Our finding will bring positive impacts to the society in terms of improved healthcare services quality, environmental and health sustainability. The findings are beneficial to local authorities either in healthcare or environmental monitoring institutions especially in the developing countries.
Association of Householder Smoking With Poverty and the Mediating Effect of NCDs in Relatively Underdeveloped Regions in China
Authors: Yang Huimin , Chen Bowen , Guo Aili , Song Jiarui , Cheng Xi , Jin Chenggang
Published: May 2022

Background:

Studies have not provided clear enough evidence on the direct association between cigarette smoking and poverty. This study aims to assess the association of householder smoking with near-poverty households, and the potential mediating effect of NCDs.

Methods

A cross-sectional survey was conducted from November 2019 to October 2020 in relatively underdeveloped regions in China. In total, 2,409 households were investigated in areas under the jurisdiction of 24 primary health care (PHC) institutions of eight provinces. Pearson's χ2-test was performed, and multivariable logistic regression and extended probit regression models were fitted to examine the association between householder smoking and near-poverty households. Moreover, generalized structural equation modeling was used to explore the mediating effect of NCDs.

Results

After adjusting for all other potential confounding factors, compared with households headed by never-smokers, households headed by smokers exhibited significantly elevated risks of being near poverty, with an odds ratio of 2.01 (95% CI: 0.48–0.91). We also found that living in rural areas and having a low education level both had a negative effect on being near poverty. Additionally, NCDs had a significantly positive mediating effect, with a 31.57% effect of householder smoking on near-poverty status mediated by NCDs; the indirect effect was estimated to be 0.17 (95% CI: 0.04–0.31).

Conclusions

Householder smoking significantly elevated the risk of the household being near poverty, and suffering NCDs had a positive mediating effect.
Systematic Bibliometric Analysis of Research Hotspots and Trends on the Application of Virtual Reality in Nursing
Authors: Zhao Junqiang , Lu Yi , Zhou Fujun , Mao Ruping , Fei Fangqin
Published: May 2022

Background

With the emergence of the metaverse, virtual reality, as a digital technology, must be getting hotter. High quality virtual reality related nursing knowledge scene learning is gradually replacing traditional education and intervention skills.

Objective

This systematic study aimed to gain insights into the overall application of virtual reality technology in the study of nursing.

Methods

Citations downloaded from the Web of Science Core Collection database for use in VR in nursing publications published from January 1, 2012, to December 31, 2021, were considered in the research. Information retrieval was analyzed using https://bibliometric.com/app, CiteSpace.5.8. R3, and VOS viewer.

Results

A total of 408 institutions from 95 areas contributed to relevant publications, of which the United States is the most influential country in this research field. The clustering labels of cited documents were obtained from the citing documents. Virtual simulation, virtual learning, clinical skills, and dementia are the clustering labels of co-cited documents. The burst keywords represented the research frontiers in 2020–2021, which were knowledge and simulation.

Conclusion

Virtual nursing has had an impact on both nurses and clients. With the emergence of the concept of the metaverse, the research and application of virtual reality technology in nursing will gradually increase.
The Role of Microbiota in Drosophila melanogaster Aging
Authors: Arias-Rojas Aranzazu , Iatsenko Igor
Published: May 2022

Intestinal microbial communities participate in essential aspects of host biology, including nutrient acquisition, development, immunity, and metabolism. During host aging, dramatic shifts occur in the composition, abundance, and function of the gut microbiota. Although such changes in the microbiota are conserved across species, most studies remain descriptive and at most suggest a correlation between age-related pathology and particular microbes. Therefore, the causal role of the microbiota in host aging has remained a challenging question, in part due to the complexity of the mammalian intestinal microbiota, most of which is not cultivable or genetically amenable. Here, we summarize recent studies in the fruit fly Drosophila melanogaster that have substantially progressed our understanding at the mechanistic level of how gut microbes can modulate host aging.
Organized Scientific Diaspora and Its Contributions to Science Diplomacy in Emerging Economies: The Case of Latin America and the Caribbean
Authors: Echeverría-King Luisa F. , Camacho Toro Reina , Figueroa Pedro , Galvis Laura A. , González Alejandra , Suárez Verónica Rossana , Torres Atencio Ivonne , Widmaier Müller Claudia Natalie
Published: May 2022

The current knowledge society has driven an unprecedented mobility of people, especially scientists, from emerging economies to developed countries. This mobility can allow the development of human talent and the access to first class infrastructure and resources, but it can also mean a loss for emerging economies due to the phenomenon of brain drain. To counteract this situation, some countries in Latin America and the Caribbean have developed models for the articulation of their scientific diaspora in projects and programs, with the aim of exchanging knowledge and capitalizing on human and technical resources to advance science, technology and innovation systems. Likewise, science diplomacy has become a tool for interlinking the work of various actors in order to advance the solution of national, transnational or global problems through scientific advice. Scientific diasporas are vital in new structures of cooperation, enabling them to innovate and solve problems jointly, advising their countries of origin and articulating policies and programs. This research seeks to analyze the interactions and initiatives identified between the organized scientific diaspora from Latin America and the Caribbean and their countries of origin in relation to science diplomacy processes, providing recommendations and proposals for public policy to improve the interaction between the diaspora and the governments of their countries of origin. Results show that diaspora organizations from Latin America and the Caribbean engage with governmental and non-state actors and are active science diplomacy stakeholders promoting the scientific developments of their country or their researchers, as well as enabling access to research resources creating alliances for scientific, institutional and academic collaborations. In the cases studied, these efforts are planned and executed by the diaspora without responding to any science diplomacy strategy of the country. Policies and programs are needed to effectively link the scientific diaspora organizations to the interests of the countries.
Reversing the Mismatch With Forefoot Striking to Reduce Running Injuries
Authors: Davis Irene S. , Chen Tony Lin-Wei , Wearing Scott C.
Published: May 2022

Recent studies have suggested that 95% of modern runners land with a rearfoot strike (RFS) pattern. However, we hypothesize that running with an RFS pattern is indicative of an evolutionary mismatch that can lead to musculoskeletal injury. This perspective is predicated on the notion that our ancestors evolved to run barefoot and primarily with a forefoot strike (FFS) pattern. We contend that structures of the foot and ankle are optimized for forefoot striking which likely led to this pattern in our barefoot state. We propose that the evolutionary mismatch today has been driven by modern footwear that has altered our footstrike pattern. In this paper, we review the differences in foot and ankle function during both a RFS and FFS running pattern. This is followed by a discussion of the interaction of footstrike and footwear on running mechanics. We present evidence supporting the benefits of forefoot striking with respect to common running injuries such as anterior compartment syndrome and patellofemoral pain syndrome. We review the importance of a gradual shift to FFS running to reduce transition-related injuries. In sum, we will make an evidence-based argument for the use of minimal footwear with a FFS pattern to optimize foot strength and function, minimize ground reaction force impacts and reduce injury risk.
Non-thermal Processing Technologies for Dairy Products: Their Effect on Safety and Quality Characteristics
Authors: Neoκleous Ioanna , Tarapata Justyna , Papademas Photis
Published: May 2022

Thermal treatment has always been the processing method of choice for food treatment in order to make it safe for consumption and to extend its shelf life. Over the past years non-thermal processing technologies are gaining momentum and they have been utilized especially as technological advancements have made upscaling and continuous treatment possible. Additionally, non-thermal treatments are usually environmentally friendly and energy-efficient, hence sustainable. On the other hand, challenges exist; initial cost of some non-thermal processes is high, the microbial inactivation needs to be continuously assessed and verified, application to both to solid and liquid foods is not always available, some organoleptic characteristics might be affected. The combination of thermal and non-thermal processing methods that will produce safe foods with minimal effect on nutrients and quality characteristics, while improving the environmental/energy fingerprint might be more plausible.
Effect of High-Quality Whole-Course Care on Psychological Status and Postoperative Pharyngeal Complications in Patients Undergoing Surgery for Hyperparathyroidism Secondary to Chronic Rrenal Failure
Authors: Luo Qinghong , Zheng Shuquan
Published: May 2022

Objective

To observe the effects of high-quality whole-course care on the psychological status and postoperative pharyngeal complications in patients undergoing surgery for secondary hyperparathyroidism (SHPT) to chronic rrenal failure (CRF).

Methods

The clinical data of 62 patients who underwent surgical treatment for CRF-SHPT from April 2018 to October 2021 in our department were retrospectively analyzed. According to the different nursing methods after admission, they were divided into two groups, of which 33 patients who received high-quality whole-course care were the high-quality group, and 29 patients who received routine nursing were the regular group. Compliance, occurrence of pharyngeal complications, improvement of preoperative and postoperative psychological status [Assessed by self-rating anxiety scale (SAS) and self-rating depression scale (SDS)], nursing satisfaction scores, and serum hormone levels [intact parathyroid hormone (iPTH), calcium (Ca), Phosphorus (P)] were compared between the two groups.

Results

The differences between the general conditions and clinical characteristics of the two groups were not significant (p > 0.05). After care, the number of cases with good compliance in the high-quality group was higher than that in the regular group, and the number of cases with non-compliance was lower than that in the regular group (p < 0.05); the difference in the number of cases with partial compliance after care between the two groups was not significant (p > 0.05). There was no significant difference in the incidence of pharyngeal complications such as sore throat, nausea and vomiting, dry throat and hoarseness between the two groups (p > 0.05); however, the 24-h postoperative sore throat and dry throat scores in the high-quality group were significantly lower than those in the regular group (p < 0.05). Patients in the high-quality group had higher nursing attitude, nursing skills, nursing safety, nursing quality, and overall nursing satisfaction scores than the regular group (p < 0.05). Compared with the pre-care period, SAS and SDS scores decreased in both groups after care, and SAS and SDS scores decreased more in the high-quality group than in the regular group (p < 0.05). Serum iPTH, Ca, and P levels decreased in both groups at 1 week after surgery, and iPTH, Ca, and P levels decreased more in the high-quality group than in the regular group (p < 0.05).

Conclusion

Through the high-quality whole-course care, full informed participation and active cooperation of CRF-SHPT patients, close medical and nursing collaboration, attention to detail and overall level of treatment can effectively improve patient compliance, psychological status and postoperative serum indicators, promote patient recovery and improve nursing satisfaction.
High Expression of TIMELESS Predicts Poor Prognosis: A Potential Therapeutic Target for Skin Cutaneous Melanoma
Authors: Zhao Shixin , Wen Shifeng , Liu Hengdeng , Zhou Ziheng , Liu Yiling , Zhong Jinbao , Xie Julin
Published: May 2022

Background

Skin cutaneous melanoma (SKCM) is the most lethal skin cancer with an increasing incidence worldwide. The poor prognosis of SKCM urgently requires us to discover prognostic biomarkers for accurate therapy. As a regulator of DNA replication, TIMELESS (TIM) has been found to be highly expressed in various malignancies but rarely reported in SKCM. The objective of this study was to evaluate the relationship between TIM and SKCM tumorigenesis and prognosis.

Methods

We obtained RNA sequencing data from TCGA and GTEx to analyze TIM expression and differentially expressed genes (DEGs). Subsequently, GO/KEGG, GSEA, immune cell infiltration analysis, and protein-protein interaction (PPI) network were used to perform the functional enrichment analysis of TIM-related DEGs. Moreover, the receiver operating characteristic (ROC) curves, Cox regression analysis, Kaplan–Meier (K-M) analysis, and nomograms were applied to figure out the clinical significance of TIM in SKCM. In addition, we investigated the relationship between TIM promoter methylation and SKCM prognosis through the UALCAN database. Finally, the immunohistochemical (IHC) results of normal skin and SKCM were analyzed to determine expression differences.

Results

TIM was significantly elevated in various malignancies, including SKCM, and high expression of TIM was associated with poor prognosis. Moreover, a total of 402 DEGs were identified between the two distinct TIM expression groups, and functional annotation showed enrichment with positive regulation of cell cycle and classic oncogenic pathways in the high TIM expression phenotype, while keratinization pathways were negatively regulated and enriched. Further analysis showed that TIM was correlated with infiltration of multiple immune cells. Finally, IHC validated the differential expression of TIM in SKCM.

Conclusion

TIM might play a pivotal role in tumorigenesis of SKCM and is closely related to its prognosis.
Bacteroidetes and Firmicutes Drive Differing Microbial Diversity and Community Composition Among Micro-Environments in the Bovine Rumen
Authors: Pinnell Lee J. , Reyes Arquimides A. , Wolfe Cory A. , Weinroth Maggie D. , Metcalf Jessica L. , Delmore Robert J. , Belk Keith E. , Morley Paul S. , Engle Terry E.
Published: May 2022

Ruminants are a critical human food source and have been implicated as a potentially important source of global methane emissions. Because of their unique digestive physiology, ruminants rely upon a symbiotic relationship with the complex and rich community of microorganism in the foregut to allow digestion of complex carbohydrates. This study used 16S rRNA gene sequencing to investigate the composition of microbial communities from three rumen micro-environments of cattle fed identical diets: (1) free fluid, (2) the fibrous pack, and (3) the mucosa. Community composition analysis revealed that while a phylogenetic core including the most abundant and most common ruminal taxa (members of Bacteroidetes and Firmicutes) existed across micro-environments, the abundances of these taxa differed significantly between fluid- and mucosa-associated communities, and specific lineages were discriminant of individual micro-environments. Members of Firmicutes, specifically Clostridiales, Lachnospiraceae, Mogibacteriaceae, Christenellaceae, and Erysipelotrichaceae were significantly more abundant in fluid communities, while members of Bacteroidetes, namely Muribaculaceae and Prevotellaceae were more abundant in mucosa-associated communities. Additionally, Methanobacteriaceae, a family of methanogenic Archaea, was more abundant in fluid-associated communities. A set of four more diverse lineages were discriminant of pack-associated communities that included Succinivibrionaceae, RFP12 (Verruco-5), Fibrobacteraceae, and Spirochaetaceae. Our findings indicate that different ecological niches within each micro-environment have resulted in significant differences in the diversity and community structure of microbial communities from rumen fluid, pack, and mucosa without the influence of diet that will help contextualize the influence of other environmental factors.
Diagnostic Investigation of 100 Cases of Abortion in Sheep in Uruguay: 2015–2021
Authors: Dorsch Matías A. , Francia María E. , Tana Leandro R. , González Fabiana C. , Cabrera Andrés , Calleros Lucía , Sanguinetti Margarita , Barcellos Maila , Zarantonelli Leticia , Ciuffo Camila , Maya Leticia , Castells Matías , Mirazo Santiago , da Silva Silveira Caroline , Rabaza Ana , Caffarena Rubén D. , Doncel Díaz Benjamín , Aráoz Virginia , Matto Carolina , Armendano Joaquín I. , Salada Sofía , Fraga Martín , Fierro Sergio , Giannitti Federico
Published: May 2022

The aim of this work was to identify causes of abortion through laboratory investigations in sheep flocks in Uruguay. One hundred cases of abortion, comprising 58 fetuses, 36 fetuses with their placentas, and 6 placentas were investigated in 2015–2021. Cases were subjected to gross and microscopic pathologic examinations, and microbiological and serological testing for the identification of causes of abortion, including protozoal, bacterial, and viral pathogens. An etiologic diagnosis was determined in 46 (46%) cases, including 33 (33%) cases caused by infectious pathogens, as determined by the detection of a pathogen along with the identification of fetoplacental lesions attributable to the detected pathogen. Twenty-seven cases (27%) were caused by Toxoplasma gondii, 5 (5%) by Campylobacter fetus subspecies fetus, and 1 (1%) by an unidentified species of Campylobacter. Fourteen cases (14%) had inflammatory and/or necrotizing fetoplacental lesions compatible with an infectious etiology. Although the cause for these lesions was not clearly identified, T. gondii was detected in 4 of these cases, opportunistic bacteria (Bacillus licheniformis, Streptococcus sp.) were isolated in 2 cases, and bovine viral diarrhea virus 1 subtype i (BVDV-1i) was detected in another. Campylobacter jejuni was identified in 1 (1%) severely autolyzed, mummified fetus. BVDV-2b was identified incidentally in one fetus with an etiologic diagnosis of toxoplasmosis. Microscopic agglutination test revealed antibodies against ≥1 Leptospira serovars in 15/63 (23.8%) fetuses; however, Leptospira was not identified by a combination of qPCR, culture, fluorescent antibody testing nor immunohistochemistry. Neospora caninum, Chlamydia abortus, Chlamydia pecorum, Coxiella burnetii and border disease virus were not detected in any of the analyzed cases. Death was attributed to dystocia in 13 (13%) fetuses delivered by 8 sheep, mostly from one highly prolific flock. Congenital malformations including inferior prognathism, a focal hepatic cyst, and enterohepatic agenesis were identified in one fetus each, the latter being the only one considered incompatible with postnatal life. Toxoplasmosis, campylobacteriosis and dystocia were the main identified causes of fetal losses. Despite the relatively low overall success rate in establishing an etiologic diagnosis, a systematic laboratory workup in cases of abortion is of value to identify their causes and enables zoonotic pathogens surveillance.
Sexual and Reproductive Health Needs and Priorities of the Adolescents in Northern Pakistan: A Formative Evaluation
Authors: Meherali Salima , Abdul Rahim Komal , Lassi Zohra S.
Published: May 2022

The vast majority (90%) of the world’s young people aged 10–24 years live in low and middle-income countries (LMICs). Pakistan has one of the world’s largest populations under 30 years. Adolescents’ access to basic sexual and reproductive health (SRH) services, such as family planning and sexuality education, remains low, especially in rural areas such as the Gilgit-Baltistan (GB) province of Northern Pakistan. This study addressed these gaps by exploring the SRH priorities and health information needs of adolescents living in GB. A cross-sectional survey was conducted with adolescents and healthcare providers. A total of 216 adolescents and 22 healthcare providers completed the survey forms. The findings pointed out that adolescents in GB have unmet SRH needs and are more interested in learning about SRH, human immunodeficiency virus (HIV) and sexually transmitted infections (STIs), and sexual abuse. The majority of the participants highlighted that their age, fear of being judged/stigmatized, and society’s attitude toward sex hinder them from opting for SRH services. The majority of healthcare providers reported that they are not well-equipped to tackle SRH-related issues and require training to better serve the adolescent SRH needs. The findings from this study have highlighted that there is a need to formulate robust interventions and strategies to raise knowledge and awareness about SRH needs among adolescents.
Exploring the Factors of Farmers’ Rural–Urban Migration Decisions in Bangladesh
Authors: Al-Maruf Abdullah , Pervez A. K. M. Kanak , Sarker Pradip Kumar , Rahman Md Saifur , Ruiz-Menjivar Jorge
Published: May 2022

In Bangladesh, rural–urban migration is widespread. Many earlier studies discussed the factors, patterns, causes, and consequences and the socio-economic and environmental impact of migration from the general perspective. However, rural–urban migration with a particular focus on particular communities or migrants’ employment profiles, for instance, farmers, is poorly described. In contrast, many farmers move from rural to urban areas every year in Bangladesh. However, the factors that affect farmers’ rural-to-urban migration are a primary concern to academia and key actors, as the country’s economy mainly depends on agriculture and farming. This paper, therefore, aimed to identify the underlying factors of the rural–urban (R–U) migration of farmers in Bangladesh. Data for this study came from phone interviews conducted with 254 migrant farmers living in city districts in Bangladesh. We adopted a three-step approach to select and identify factors that impacted farmers’ decision to move from rural to urban settings. First, we reviewed the extant literature and compiled more than 70 variables of interest relevant to farmers’ migration. Second, 30 variables were selected for data collection after consultations with key informants (KIIs) and informal discussions (IDs) with farmers and local community leaders. Besides, the Q-methodology was used to assess the level of importance of the selected variables. Lastly, principal component analysis (PCA) was performed to extract salient dimensions of farmers’ rural-to-urban migration, where 21 variables were detected that consistently exceeded a threshold value of 0.50 of communality for further analysis. Our findings show that six dimensions—i.e., individual, household, economic, attitudinal, spatial, and climate-induced extremes—significantly influence and contribute to rural urban migration decisions for farmers. Further, our results indicated that age, agricultural knowledge, household debt, seasonal famine/poverty (Monga), unemployment in rural areas, availability of anticipated job opportunities in urban areas, shortage of agricultural inputs, and river erosion significantly influenced farmers’ decision to leave their farms in Bangladesh. Findings from this study may be used as inputs in predictive models and benchmark guidelines for assessing trends and patterns of rural-to-urban migration and for the formulation of policy and programs targeting domestic migration in Bangladesh for proper urban planning and further rural development.
Trichomonosis in Austrian Songbirds—Geographic Distribution, Pathological Lesions and Genetic Characterization over Nine Years
Authors: Brunthaler René , Teufelbauer Norbert , Seaman Benjamin , Nedorost Nora , Bittermann Karin , Matt Julia , Weissenbacher-Lang Christiane , Weissenböck Herbert
Published: May 2022

In the early summer of 2012, sudden mass mortality among songbirds, particularly in greenfinches (Chloris chloris, syn: Carduelis chloris) was observed in Austria, which was caused by the protozoan parasite Trichomonas gallinae. This pathogen induced fibrinonecrotic ingluvitis and/or esophagitis, leading to impairment of food intake and ultimately death due to starvation. The pathogen was successfully detected within the lesions by polymerase chain reaction (PCR) and chromogenic in situ hybridization. The epizootic resulted in a significant decline in the Austrian greenfinch population. Continuing passive surveillance in the subsequent years (2013–2020) revealed that the condition occurred each year and was present in the entire country. Genetic characterization of the pathogen showed the presence of an identical strain irrespective of geographical location, bird species, and year.
The Emergence and Dissemination of Multidrug Resistant Pseudomonas aeruginosa in Lebanon: Current Status and Challenges during the Economic Crisis
Authors: Dabbousi Ahmad Ayman , Dabboussi Fouad , Hamze Monzer , Osman Marwan , Kassem Issmat I.
Published: May 2022

Pseudomonas aeruginosa is a common cause of healthcare-associated infections and chronic airway diseases in non-clinical settings. P. aeruginosa is intrinsically resistant to a variety of antimicrobials and has the ability to acquire resistance to others, causing increasingly recalcitrant infections and elevating public health concerns. We reviewed the literature on multidrug-resistant (MDR) P. aeruginosa isolated from humans (nosocomial and community-associated), animals, and the environment in Lebanon, a country that has been suffering from a surge in antimicrobial resistance (AMR). We identified 24 studies that described the epidemiology and antimicrobial susceptibility profiles of P. aeruginosa. Our analysis showed that the bacterium was predominant in lesions of patients on mechanical ventilation and in burn patients and those with diabetic foot infections and hematological malignancies. We also found that carbapenem resistance in P. aeruginosa isolates in Lebanon involved both enzymatic and non-enzymatic mechanisms but depended predominantly on VIM-2 production (40.7%). Additionally, MDR P. aeruginosa was detected in animals, where a recent study reported the emergence of carbapenemase-producing P. aeruginosa in livestock in Lebanon. Notably, no studies evaluated the contribution of MDR P. aeruginosa in the environment to human infections. Taken together, our findings highlight the need for AMR surveillance programs and a national action plan to combat resistance in Lebanon.
Phenotypic Adaptation to Antiseptics and Effects on Biofilm Formation Capacity and Antibiotic Resistance in Clinical Isolates of Early Colonizers in Dental Plaque
Authors: Auer David L. , Mao Xiaojun , Anderson Annette Carola , Muehler Denise , Wittmer Annette , von Ohle Christiane , Wolff Diana , Frese Cornelia , Hiller Karl-Anton , Maisch Tim , Buchalla Wolfgang , Hellwig Elmar , Al-Ahmad Ali , Cieplik Fabian
Published: May 2022

Despite the wide-spread use of antiseptics in dental practice and oral care products, there is little public awareness of potential risks associated with antiseptic resistance and potentially concomitant cross-resistance. Therefore, the aim of this study was to investigate potential phenotypic adaptation in 177 clinical isolates of early colonizers of dental plaque (Streptococcus, Actinomyces, Rothia and Veillonella spp.) upon repeated exposure to subinhibitory concentrations of chlorhexidine digluconate (CHX) or cetylpyridinium chloride (CPC) over 10 passages using a modified microdilution method. Stability of phenotypic adaptation was re-evaluated after culture in antiseptic-free nutrient broth for 24 or 72 h. Strains showing 8-fold minimal inhibitory concentration (MIC)-increase were further examined regarding their biofilm formation capacity, phenotypic antibiotic resistance and presence of antibiotic resistance genes (ARGs). Eight-fold MIC-increases to CHX were detected in four Streptococcus isolates. These strains mostly exhibited significantly increased biofilm formation capacity compared to their respective wild-type strains. Phenotypic antibiotic resistance was detected to tetracycline and erythromycin, consistent with the detected ARGs. In conclusion, this study shows that clinical isolates of early colonizers of dental plaque can phenotypically adapt toward antiseptics such as CHX upon repeated exposure. The underlying mechanisms at genomic and transcriptomic levels need to be investigated in future studies.
The Implementation of National Action Plan (NAP) on Antimicrobial Resistance (AMR) in Bangladesh: Challenges and Lessons Learned from a Cross-Sectional Qualitative Study
Authors: Ahmed Syed Masud , Naher Nahitun , Tune Samiun Nazrin Bente Kamal , Islam Bushra Zarin
Published: May 2022

This study explored the current situation of the National Action Plan (NAP) on Antimicrobial Resistance (AMR) implementation in Bangladesh and examined how different sectors (human, animal, and environment) addressed the AMR problem in policy and practice, as well as associated challenges and barriers to identifying policy lessons and practices. Informed by a rapid review of the available literature and following the World Health Organization (WHO) AMR situation analysis framework, a guideline was developed to conduct in-depth interviews with selected stakeholders from January to December 2021. Data were analysed using an adapted version of Anderson’s governance framework. Findings reveal the absence of required inter-sectoral coordination essential to a multisectoral approach. There was substantial coordination between the human health and livestock/fisheries sectors, but the environment sector was conspicuously absent. The government initiated some hospital-based awareness programs and surveillance activities, yet no national Monitoring and Evaluation (M&E) framework was established for NAP activities. Progress of implementation was slow, constrained by the shortage of a trained health workforce and financial resources, as well as the COVID-19 pandemic. To summarise, five years into the development of the NAP in Bangladesh, its implementation is not up to the level that the urgency of the situation requires. The policy and practice need to be cognisant of this fact and do the needful things to avoid a catastrophe.
Method for Calculating Vertical Compression Bearing Capacity of the Static Drill Rooted Nodular Pile
Authors: Guo Jing , Dai Guoliang , Wang Yue
Published: May 2022

The static drill rooted nodular (SDRN) pile is a new kind of composite pile foundation made by inserting a precast nodular pile into the cemented soil. Based on the tests and analysis on the mechanism characteristics of these two kinds of interfaces, which are between the pile and cemented soil, and between the cemented soil and soil, this paper proposes a calculation method for the ultimate vertical bearing capability of the SDRN pile considering two failure modes. When the precast pile and surrounding cemented soil fails as a whole, the diameter of the cemented soil pile is taken to calculate the ultimate shaft resistance, and the shaft resistance of the SDRN pile is about 1.05∼1.10 times that of the bored pile in the same soil layer. When the core precast pile fails in penetration mode, the diameter of the core pile is taken. The pile shaft resistance takes that of displacement piles in the same soil layer. The lower nodular pile shaft resistance should consider the squeezing effect of nodular joints. Besides, the improvement of pile tip resistance due to the expanded cemented soil should also be taken into consideration. The result is the smaller value calculated according to these two failure modes. The ultimate bearing capacity of a SDRN pile calculated by the theoretical method is not only compared with the field test result, but also the simulation result of a 2D model pile built by PLAXIS finite element software. In addition, the finite element simulation is also confirmed to be an effective way to investigate the mechanism characteristics of SDRN piles more thoroughly.