bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Effect of Different Concentrations of Spirotetramat on the Diaspine Scale Parlatoria ziziphi in Citrus Orchards
Authors: Assouguem Amine , Kara Mohammed , Mechchate Hamza , AlZain Mashail N. , Noman Omar Mohammed , Imtara Hamada , Hano Christophe , Ibrahim Mansour N. , Benmessaoud Safaâ , Farah Abdellah , Lazraq Abderahim
Published: September 2021

The control of Parlatoria ziziphi (Lucas, 1853) was studied in citrus orchards at Mechraa Belksiri in the Gharb area of Morocco. Three concentrations of spirotetramat T0 = 0 L/Ha as a control experiment, T1 = 0.625 L/Ha, T2 = 0.755 L/Ha, and T3 = 1 L/Ha, were applied on 4 ha of Valencia late orchard (each dose for 1 ha of Valencia late). The effect of spirotetramat was evaluated on the mortalities and survival rates of P. ziziphin during the stages of larvae (first instar and second instar) and females (F1, F2, and F3). Results showed that the spirotetramat was effective on larvae and females of P. ziziphi. Among the 11,229 females recorded, 93% were inhibited, while only 7% were intact after the treatment period. Finally, our study highlights that all concentrations tested were effective on the P. ziziphi population; besides, all three concentrations of this product tested were equally effective on larvae and females of P. ziziphi. Thus, during the spread period, spraying a low concentration of this product (0.625 L/Ha) will better control this pest and reduce the environmental impact.
The Impact of Global Warming on the Winter Wheat Production of China
Authors: Zhang Yu , Qiu Xiaolei , Yin Tingwei , Liao Zhiyi , Liu Bing , Liu Leilei
Published: September 2021

The impact of global warming on crop growth periods and yields has been evaluated by using crop models, which need to provide various kinds of input datasets and estimate numerous parameters before simulation. Direct studies on the changes of climatic factors on the observed crop growth and yield could provide a more simple and intuitive way for assessing the impact of climate change on crop production. In this study, four cultivars which were planted over more than 15 years in eight test stations in the Northern Winter Wheat Region of China were selected to investigate the relationships between growth periods, grain yields, yield components and temperatures. It was found that average temperatures and heat degree-days (HDD) during the winter wheat growing seasons tended to increase over time series at most study sites. The length of growth period and growing degree days (GDD) were not fixed for a given cultivar among different years and locations, and the variation on the periods from sowing to jointing was relatively greater than in the other periods. The increasing temperature mainly shortened the periods from sowing to jointing and jointing to anthesis, which led to the decrease in entire growth periods. Positive relationships between spike number, grain number per spike, grain yields and average temperatures were identified in the Northern Winter Wheat Region of China. The grain yield in the study area increased by 406.3 kg ha−1 for each 1 °C increase in average temperature. Further, although the positive relationship between grain yield and HDD was found in our study, the heat stress did not lead to the wheat yield decline in the study region. Temperature is a major determinant of wheat growth and development, the average temperature and the frequency of heat stresses are projected to increase in the future, so understanding the effect of temperature on wheat production and adopting appropriate adaptation are required for the implementation of food security policies.
Applications of CRISPR/Cas9 System in Vegetatively Propagated Fruit and Berry Crops
Authors: Fizikova Anastasia , Tikhonova Nadezhda , Ukhatova Yulia , Ivanov Roman , Khlestkina Elena
Published: September 2021

Fruit and berry crops, as well as grapes, are important parts of the human diet and, at the same time, significant objects of genetic, breeding, biochemical and nutritional research. Traditional approaches of crop research and improvement are now complemented by effective modern genetic technologies. In this review, we analyze and summarize the achievements in genome editing of fruit, berry crops and grapes. New approaches accelerate the improvement of genotypes for many groups of traits: plant resistance to unfavorable environmental factors, flowering and ripening time, plant architectonics, fruit shelf time and biochemical composition. Genome editing using the CRISPR/Cas9 system has been successfully tested on the most important vegetatively propagated fruit and berry crops (apple, pear, orange, kumquat, grapefruit, banana, strawberry and kiwi) and grapes. About 30 genes of these crops have been used as targets for the introduction of desired mutations using the CRISPR/Cas9 system. The most valuable results are the improvement of important agronomic traits. For 24 genes it has been shown that their knockout can result in the improvement of varieties. In addition, the review pays attention to the comparative analysis of the explant types of vegetatively propagated crops used for the delivery of editing genetic constructs, as well as the comparison of the editing efficiency depending on the variation of the objects used, delivery methods, etc. The article discusses the existing limitations that need to be overcome for a wider application of genomic editing in order to improve varieties of fruit and berry crops, as well as grapes.
Rapid Detection of Multiple Classes of β-Lactam Antibiotics in Blood Using an NDM-1 Biosensing Assay
Authors: Meng Qinglai , Wang Yao , Long Yali , Yue Aiping , Mecklenburg Michael , Tian Shuaiyan , Fu Yujia , Yao Xiangyu , Liu Jianyi , Song Dewei , Wu Changxin , Xie Bin
Published: September 2021

Currently, assays for rapid therapeutic drug monitoring (TDM) of β-lactam antibiotics in blood, which might be of benefit in optimizing doses for treatment of critically ill patients, remain challenging. Previously, we developed an assay for determining the penicillin-class antibiotics in blood using a thermometric penicillinase biosensor. The assay eliminates sample pretreatment, which makes it possible to perform semicontinuous penicillin determinations in blood. However, penicillinase has a narrow substrate specificity, which makes it unsuitable for detecting other classes of β-lactam antibiotics, such as cephalosporins and carbapenems. In order to assay these classes of clinically useful antibiotics, a novel biosensor was developed using New Delhi metallo-β-lactamase-1 (NDM-1) as the biological recognition layer. NDM-1 has a broad specificity range and is capable of hydrolyzing all classes of β-lactam antibiotics in high efficacy with the exception of monobactams. In this study, we demonstrated that the NDM-1 biosensor was able to quantify multiple classes of β-lactam antibiotics in blood plasma at concentrations ranging from 6.25 mg/L or 12.5 mg/L to 200 mg/L, which covered the therapeutic concentration windows of the tested antibiotics used to treat critically ill patients. The detection of ceftazidime and meropenem was not affected by the presence of the β-lactamase inhibitors avibactam and vaborbactam, respectively. Furthermore, both free and protein-bound β-lactams present in the antibiotic-spiked plasma samples were detected by the NDM-1 biosensor. These results indicated that the NDM-1 biosensor is a promising technique for rapid TDM of total β-lactam antibiotics present in the blood of critically ill patients.
Supplementation of Live Yeast, Mannan Oligosaccharide, and Organic Selenium during the Adaptation Phase of Newly Arrived Beef Cattle: Effects on Health Status, Immune Functionality, and Growth Performance
Authors: Grossi Silvia , Dell’Anno Matteo , Rossi Luciana , Compiani Riccardo , Sgoifo Rossi Carlo Angelo
Published: September 2021

The effect of a nutraceutical mixture, based on live yeast (LY), mannan oligosaccharides (MOS) and organic selenium (Se) on health status, as well as immune functionality and growth performance in the fattening of newly received beef cattle, was evaluated. A total of 1036 Charolaise heifers were allocated into two experimental groups: (i) control group, without any nutraceutical support (n = 487; initial weight = 325 ± 21 kg); and (ii) treatment group, supplementation during the first 30 days, with LY (5 g/head/day), organic Se (3 mg/head/day), and MOS (10 g/head/day) (n = 549; initial weight = 323 ± 23 kg). The incidence of bovine respiratory disease (BRD) and other health issues was monitored, as well as the mortality rate. Blood samples were taken at d0 and d30 to evaluate the immune functionality and the inflammatory status. Growth performances, feces chemical composition, and carcass characteristics were recorded. The BRD occurrence tended to be reduced (p = 0.06) in the Treatment group. The BHV-1 antibody production after vaccination was significantly improved (p = 0.031), as well as the bactericidal activity (p = 0.0012) in the Treatment group. No differences were found in the inflammatory status parameters. The final weight (p = 0.006) and the average daily gain at d30 (p < 0.0001) were significantly improved by the treatment. No differences were found in terms of carcass characteristics, while the fecal content of NDF (p < 0.0001), ADF (p = 0.0003), and starch (p < 0.0001) were significantly reduced by the treatment. The result of the present study suggests that the nutraceutical mixture used can support the animal’s immune systems, improving its ability to react against pathogens, as well as feed efficiency and growth performances during the whole fattening period.
Positive Interaction between H2O2 and Ca2+ Mediates Melatonin-Induced CBF Pathway and Cold Tolerance in Watermelon (Citrullus lanatus L.)
Authors: Chang Jingjing , Guo Yanliang , Li Jiayue , Su Zhuangzhuang , Wang Chunxia , Zhang Ruimin , Wei Chunhua , Ma Jianxiang , Zhang Xian , Li Hao
Published: September 2021

Cold stress is a major environmental factor that detrimentally affects plant growth and development. Melatonin has been shown to confer plant tolerance to cold stress through activating the C-REPEAT BINDING FACTOR (CBF) pathway; however, the underlying modes that enable this function remain obscure. In this study, we investigated the role of H2O2 and Ca2+ signaling in the melatonin-induced CBF pathway and cold tolerance in watermelon (Citrullus lanatus L.) through pharmacological, physiological, and genetic approaches. According to the results, melatonin induced H2O2 accumulation, which was associated with the upregulation of respiratory burst oxidase homolog D (ClRBOHD) during the early response to cold stress in watermelon. Besides, melatonin and H2O2 induced the accumulation of cytoplasmic free Ca2+ ([Ca2+]cyt) in response to cold. This was associated with the upregulation of cyclic nucleotide-gated ion channel 2 (ClCNGC2) in watermelon. However, blocking of Ca2+ influx channels abolished melatonin- or H2O2-induced CBF pathway and cold tolerance. Ca2+ also induced ClRBOHD expression and H2O2 accumulation in early response to cold stress in watermelon. Inhibition of H2O2 production in watermelon by RBOH inhibitor or in Arabidopsis by AtRBOHD knockout compromised melatonin-induced [Ca2+]cyt accumulation and melatonin- or Ca2+-induced CBF pathway and cold tolerance. Overall, these findings indicate that melatonin induces RBOHD-dependent H2O2 generation in early response to cold stress. Increased H2O2 promotes [Ca2+]cyt accumulation, which in turn induces H2O2 accumulation via RBOHD, forming a reciprocal positive-regulatory loop that mediates melatonin-induced CBF pathway and subsequent cold tolerance.
Differential Redox State and Iron Regulation in Chronic Obstructive Pulmonary Disease, Acute Respiratory Distress Syndrome and Coronavirus Disease 2019
Authors: Duca Lorena , Ottolenghi Sara , Coppola Silvia , Rinaldo Rocco , Dei Cas Michele , Rubino Federico Maria , Paroni Rita , Samaja Michele , Chiumello Davide Alberto , Motta Irene
Published: September 2021

In patients affected by Acute Respiratory Distress Syndrome (ARDS), Chronic Obstructive Pulmonary Disease (COPD) and Coronavirus Disease 2019 (COVID-19), unclear mechanisms negatively interfere with the hematopoietic response to hypoxia. Although stimulated by physiological hypoxia, pulmonary hypoxic patients usually develop anemia, which may ultimately complicate the outcome. To characterize this non-adaptive response, we dissected the interplay among the redox state, iron regulation, and inflammation in patients challenged by either acute (ARDS and COVID-19) or chronic (COPD) hypoxia. To this purpose, we evaluated a panel of redox state biomarkers that may integrate the routine iron metabolism assays to monitor the patients’ inflammatory and oxidative state. We measured redox and hematopoietic regulators in 20 ARDS patients, 20 ambulatory COPD patients, 9 COVID-19 ARDS-like patients, and 10 age-matched non-hypoxic healthy volunteers (controls). All the examined pathological conditions induced hypoxia, with ARDS and COVID-19 depressing the hematopoietic response without remarkable effects on erythropoietin. Free iron was higher than the controls in all patients, with higher levels of hepcidin and soluble transferrin receptor in ARDS and COVID-19. All markers of the redox state and antioxidant barrier were overexpressed in ARDS and COVID-19. However, glutathionyl hemoglobin, a candidate marker for the redox imbalance, was especially low in ARDS, despite depressed levels of glutathione being present in all patients. Although iron regulation was dysfunctional in all groups, the depressed antioxidant barrier in ARDS, and to a lesser extent in COVID-19, might induce greater inflammatory responses with consequent anemia.
Using High-Pressure Technology to Develop Antioxidant-Rich Extracts from Bravo de Esmolfe Apple Residues
Authors: Bordalo Mário , Seabra Inês J. , Silva Andreia Bento , Terrasso Ana Paula , Brito Catarina , Serra Margarida , Bronze Maria R. , Duarte Catarina M. M. , Braga Mara E. M. , de Sousa Hermínio C. , Serra Ana Teresa
Published: September 2021

Bravo de Esmolfe (BE) is a traditional Portuguese apple highly appreciated by consumers due to its peculiar flavor and aroma. This apple contains higher concentration of phenolic compounds than other cultivars and is thus considered a rich source of antioxidants. Its sensorial and functional properties have attracted farmers’ associations to increase BE production. However, a large quantity of apples is wasted due to storage/transportation procedures that impact BE’s quality attributes. In this work, we applied high-pressure extraction methodologies to generate antioxidant-rich fractions from BE residues aiming at adding high value to these agro-food by-products. We performed a first extraction step using supercritical CO2, followed by a second extraction step where different CO2 + ethanol mixtures (10–100% v/v) were tested. All experiments were carried out at 25 MPa and 50 °C. Extracts were characterized in terms of global yield, phenolic content and antioxidant activity using chemical (ORAC, HOSC, HORAC) and cell-based assays (CAA). We demonstrated that, although the pressurized 100% ethanol condition promoted the highest recovery of phenolic compounds (509 ± 8 mg GAE/100 g BE residues), the extract obtained with 40% ethanol presented the highest CAA (1.50 ± 0.24 µmol QE/g dw) and ORAC (285 ± 16 µmol TEAC/g dw), as well as HOSC and HORAC values, which correlated with its content of epicatechin and procyanidin B2. Noteworthy, this fraction inhibited free radical production in human neurospheroids derived from NT2 cells, a robust 3D cell model for neuroprotective testing.
Oxidative Stress and Related Biomarkers in Gilbert’s Syndrome: A Secondary Analysis of Two Case-Control Studies
Authors: Wagner Karl-Heinz , Seyed Khoei Nazlisadat , Hana Claudia , Doberer Daniel , Marculescu Rodrig , Bulmer Andrew , Hörmann-Wallner Marlies , Mölzer Christine
Published: September 2021

Bilirubin is an important antioxidant and a modulator of biological functions. However, most of the protection against oxidative stress was shown in vitro or ex vivo. The aim of this case-control study was to investigate whether subjects with Gilbert’s syndrome (GS) experience different levels of lipid and protein oxidation (as well as differences in oxidative stress related markers) compared to healthy controls. GS subjects (n = 119) demonstrated higher serum levels of unconjugated bilirubin (p < 0.001), a lower BMI (p < 0.001), 37% higher antioxidant potential assessed as ferric reducing ability potential (p < 0.001), higher advanced oxidation protein products (p < 0.01) andlower apolipoprotein B (p < 0.05), hs-C-reactive protein (p < 0.05), interleukin 6 (p < 0.001) and interleukin 1 beta (p < 0.05) values compared to healthy controls (n = 119). Furthermore, the resting heart rate was significantly lower in the GS group (p < 0.05). Stronger protective effects for GS subjects were demonstrated in the older subgroup (n = 104, average age 50 years) compared to those of the younger group (n = 134, average age 27 years). Although not all markers related to oxidative stress were different between the groups (e.g., malondialdehyde, homocysteine, oxLDL, and myeloperoxidase; p > 0.05), the observed differences contribute to the explanation of why GS serves as an important protector in the pathogenesis of metabolic, oxidative stress related diseases.
Optical Turbulence Profile Forecasting and Verification in the Offshore Atmospheric Boundary Layer
Authors: Xu Manman , Shao Shiyong , Liu Qing , Sun Gang , Han Yong , Weng Ningquan
Published: September 2021

A backpropagation neural network (BPNN) approach is proposed for the forecasting and verification of optical turbulence profiles in the offshore atmospheric boundary layer. To better evaluate the performance of the BPNN approach, the Holloman Spring 1999 thermosonde campaigns (HMNSP99) model for outer scale, and the Hufnagel/Andrew/Phillips (HAP) model for a single parameter are selected here to estimate profiles. The results have shown that the agreement between the BPNN approach and the measurement is very close. Additionally, statistical operators are used to quantify the performance of the BPNN approach, and the statistical results also show that the BPNN approach and measured profiles are consistent. Furthermore, we focus our attention on the ability of the BPNN approach to rebuild integrated parameters, and calculations show that the BPNN approach is reliable. Therefore, the BPNN approach is reasonable and remarkable for reconstructing the strength of optical turbulence of the offshore atmospheric boundary layer.
An Analytical Study on the Pull-Out Strength of Anchor Bolts Embedded in Concrete Members by SPH Method
Authors: Lu Chi , Sonoda Yoshimi
Published: September 2021

As an important method for connecting structural members, anchor bolts have been installed in many situations. Therefore, accurate evaluation of the pull-out strength of anchor bolts has always been an important issue, considering the complicated actual installation conditions and the problem of aging deterioration of the structural members. In general, the patterns of pull-out failure of anchor bolts can be classified into three types: adhesion failure, cone failure, and bolt break. However, it sometimes shows a mixed fracture pattern, and it is not always easy to predict the accurate pull-out strength. In this study, we attempted to evaluate the pull-out strength of anchor bolts under various installation conditions using SPH, which can analyze the crack growth process in the concrete. In particular, the anchor bolt-concrete interface model was introduced to SPH analysis in order to consider the bond failure, and it was confirmed that various failure patterns and the load capacity could be predicted by proposed SPH method. After that, the influence of several parameters, such as bond stress limit, anchor bolt diameter, and the anchor bolt embedment depth on the failure patterns and the load capacity, were investigated by numerical calculation. Furthermore, several useful suggestions on the pull-out strength of anchor bolts under improper installation conditions, such as the ends of members for the purpose of seismic retrofitting, are presented.
A Water/Ion Separation Device: Theoretical and Numerical Investigation
Authors: Sofos Filippos
Published: September 2021

An array of ion separation cells is presented in this work, to propose a novel desalination device. Molecular Dynamics simulations have been incorporated to establish the theoretical background and calculate all parameters that could lead the manufacturing step. The main system component is an ion separation cell, in which water/NaCl solution flows due to an external pressure difference and ions are directed towards the non-permeable walls under the effect of an electric field, with direction perpendicular to the flow. Clean water is gathered from the output, while the remaining, high-concentration water/ion solution is re-cycled in the cells. The strength of the electric field, cell dimensions, and wall/fluid interactions are investigated over a wide range, and shear viscosity and the volumetric flow rate are calculated for each case.
Multi-Criteria Decision Making Model for Application Maintenance Offshoring Using Analytic Hierarchy Process
Authors: Rahman Hanif Ur , Raza Mushtaq , Afsar Palwasha , Alharbi Abdullah , Ahmad Sultan , Alyami Hashym
Published: September 2021

The phenomenon of Global Software Development (GSD) has attracted the interest of businesses all over the world. It brings together partners from various national and corporate cultures to develop applications with numerous advantages, including access to a vast labor pool, cost savings, and round the clock growth. GSD, on the other hand, is technologically and organizationally diverse and poses a number of obstacles for the development team, such as geographical distance, cultural differences, communication and language barriers. Global services are provided by selecting one of the suitable global delivery options, i.e., the onshore model, nearshore model or offshore model. Experts typically choose one of the models based on the nature of the project and the needs of the customer. However, the vendors and clients lack an adequate decision support system that can assist them in making suitable sourcing decisions. Therefore, the current study presents a Multi-Criteria Decision Making (MCDM) model for offshore outsourcing decisions of application maintenance. To achieve our target, two systematic literature reviews were conducted that explored a list of 15 influencing factors. The identified factors were further evaluated in the outsourcing industry by performing an empirical study that resulted in a list of 10 critical success factors. We propose a sourcing framework based on the critical success factors that can assist decision makers in adopting a suitable sourcing strategy for the offshore outsourcing of application maintenance. In order to further enhance the decision-making process, the MCDM model is developed based on the Analytic Hierarchy Process (AHP). The MCDM model is evaluated with three case studies in highly reputable international companies, including IBM Stockholm, Sweden, Vattenfall AB, Stockholm, Sweden and a London based company in the United Kingdom. The outcomes of these case studies are further reviewed and validated by the outsourcing specialists in other firms. The proposed model is used as a decision support system that determines the ranking of sourcing alternatives and suggests the most suitable option for application maintenance offshoring.
Revisiting Text Guide, a Truncation Method for Long Text Classification
Authors: Fiok Krzysztof , Karwowski Waldemar , Gutierrez Edgar , Davahli Mohammad Reza , Wilamowski Maciej , Ahram Tareq
Published: September 2021

The quality of text classification has greatly improved with the introduction of deep learning, and more recently, models using attention mechanism. However, to address the problem of classifying text instances that are longer than the length limit adopted by most of the best performing transformer models, the most common method is to naively truncate the text so that it meets the model limit. Researchers have proposed other approaches, but they do not appear to be popular, because of their high computational cost and implementation complexity. Recently, another method called Text Guide has been proposed, which allows for text truncation that outperforms the naive approach and simultaneously is less complex and costly than earlier proposed solutions. Our study revisits Text Guide by testing the influence of certain modifications on the method’s performance. We found that some aspects of the method can be altered to further improve performance and confirmed several assumptions regarding the dependence of the method’s quality on certain factors.
Physically Consistent Scar Tissue Dynamics from Scattered Set of Data: A Novel Computational Approach to Avoid the Onset of the Runge Phenomenon
Authors: Sergi Pier Nicola , De la Oliva Natalia , del Valle Jaume , Navarro Xavier , Micera Silvestro
Published: September 2021

The foreign body reaction is a complex biological process leading to the insulation of implanted artificial materials through a capsule of scar tissue. In particular, in chronic implantations of neural electrodes, the prediction of the scar tissue evolution is crucial to assess the implant reliability over time. Indeed, the capsule behaves like an increasing insulating barrier between electrodes and nerve fibers. However, no explicit and physically based rules are available to computationally reproduce the capsule evolution. In addition, standard approaches to this problem (i.e., Vandermonde-based and Lagrange interpolation) fail for the onset of the Runge phenomenon. More specifically, numerical oscillations arise, thus standard procedures are only able to reproduce experimental detections while they result in non physical values for inter-interval times (i.e., times before and after experimental detections). As a consequence, in this work, a novel framework is described to model the evolution of the scar tissue thickness, avoiding the onset of the Runge phenomenon. This approach is able to provide novel approximating functions correctly reproducing experimental data (R2≃0.92) and effectively predicting inter-interval detections. In this way, the overall performances of previous approaches, based on phenomenological fitting polynomials of low degree, are improved.
Low Temperature Resistance Increase for Bitumen by Compounding with Tar
Authors: Teltayev Bagdat , Seilkhanov Tulegen , Oliviero Rossi Cesare , Amirbayev Yerik , Begaliyeva Sakhypzhamal
Published: September 2021

In this paper, a conventional road bitumen with penetration grade 100–130 is compounded with tar in order to obtain bitumen with improved low temperature resistance. The low temperature (at −24 °C, −30 °C and −36 °C) resistance of the virgin bitumen and the compounded one is evaluated by testing on a bending beam rheometer. It was found that the optimum compounding (20% of tar by weight) decreases the stiffness essentially (from 18% to 34%), i.e., it increases the low temperature resistance of the bitumen. The stiffness decreases in the compounded bitumen can be explained by quantitative variations in its group chemical composition and molecular fragments. Group chemical composition has been determined by the method of absorption chromatography, and the fragments of molecules are identified by NMR-spectroscopy.
The Effect of Carbonation Level on the Acceptability and Purchase Intent of Muscadine and Fruit Wines
Authors: Wendrick Nicholas A. , Sims Charles A. , MacIntosh Andrew J.
Published: September 2021

Carbonation is a value-added process that can affect the mouthfeel, perception of volatile compounds, perception of sweetness, and ultimately if a consumer likes a wine. While much work has been completed on traditional varieties of sparkling wine, little research has been completed on niche market wines such as muscadine and fruit wines, which make up a large percentage of wines produced in the Southeastern USA. The objective of this research was to create and evaluate force-carbonated sparkling wine at five carbonation levels. Five finished wines from Florida wineries were obtained, then assessed for the sugar and alcohol content. Each wine was carbonated and then presented to consumers for sensory evaluation (n = 68–89 per evaluation). The questionnaire assessed the perceived sweetness, preference, liking, purchase intent, and comments of each sample. The data illustrated participants consistently preferred the carbonated samples over the noncarbonated sample. The data indicates a roughly even distribution of preference between the four carbonation levels. The data also showed statistically significant differences between the original wine and the carbonated varieties with respect to liking, preference, and purchase intent, which was supported by the consumer’s comments for the most preferred and least preferred samples. Overall, this research serves to impact the wine industry by identifying how carbonation levels affect the acceptability of niche wine varieties, and allows winemakers to successfully expand, diversify, and increase the product portfolio for wineries.
Enhanced Cancer Starvation Therapy Based on Glucose Oxidase/3-Methyladenine-Loaded Dendritic Mesoporous OrganoSilicon Nanoparticles
Authors: Wu Fan , Liu Yang , Cheng Hui , Meng Yun , Shi Jieyun , Chen Yang , Wu Yelin
Published: September 2021

Cell autophagy is a well-known phenomenon in cancer, which limits the efficacy of cancer therapy, especially cancer starvation therapy. Glucose oxidase (GOx), which is considered as an attractive starvation reagent for cancer therapy, can effectively catalyze the conversion of glucose into gluconic acid and hydrogen peroxide (H2O2) in the presence of O2. However, tumor cells adapt to survive by inducing autophagy, limiting the therapy effect. Therefore, anti-cell adaptation via autophagy inhibition could be used as a troubleshooting method to enhance tumor starvation therapy. Herein, we introduce an anti-cell adaptation strategy based on dendritic mesoporous organosilica nanoparticles (DMONs) loaded with GOx and 3-methyladenine (3-MA) (an autophagy inhibition agent) to yield DMON@GOx/3-MA. This formulation can inhibit cell adaptative autophagy after starvation therapy. Our in vitro and in vivo results demonstrate that autophagy inhibition enhances the efficacy of starvation therapy, leading to tumor growth suppression. This anti-cell adaptation strategy will provide a new way to enhance the efficacy of starvation cancer therapy.
Recent Trends in Exhaled Breath Diagnosis Using an Artificial Olfactory System
Authors: Kim Chuntae , Raja Iruthayapandi Selestin , Lee Jong-Min , Lee Jong Ho , Kang Moon Sung , Lee Seok Hyun , Oh Jin-Woo , Han Dong-Wook
Published: September 2021

Artificial olfactory systems are needed in various fields that require real-time monitoring, such as healthcare. This review introduces cases of detection of specific volatile organic compounds (VOCs) in a patient’s exhaled breath and discusses trends in disease diagnosis technology development using artificial olfactory technology that analyzes exhaled human breath. We briefly introduce algorithms that classify patterns of odors (VOC profiles) and describe artificial olfactory systems based on nanosensors. On the basis of recently published research results, we describe the development trend of artificial olfactory systems based on the pattern-recognition gas sensor array technology and the prospects of application of this technology to disease diagnostic devices. Medical technologies that enable early monitoring of health conditions and early diagnosis of diseases are crucial in modern healthcare. By regularly monitoring health status, diseases can be prevented or treated at an early stage, thus increasing the human survival rate and reducing the overall treatment costs. This review introduces several promising technical fields with the aim of developing technologies that can monitor health conditions and diagnose diseases early by analyzing exhaled human breath in real time.
Direct Selective Oxidation of Hydrogen Sulfide: Laboratory, Pilot and Industrial Tests
Authors: Khairulin Sergei , Kerzhentsev Mikhail , Salnikov Anton , Ismagilov Zinfer R.
Published: September 2021

This article is devoted to scientific and technical aspects of the direct catalytic oxidation of hydrogen sulfide for the production of elemental sulfur. It includes a detailed description of the Claus process as the main reference technology for hydrogen sulfide processing methods. An overview of modern catalytic systems for direct catalytic oxidation technology and known processes is presented. Descriptions of the scientific results of the Institute of Catalysis of the SB RAS in a study of the physical and chemical foundations of the process and the creation of a catalyst for it are included. The Boreskov Institute of Catalysis SB RAS technologies based on fundamental studies and their pilot and industrial testing results are described.
Comparison of Lower and Upper Quarter Y Balance Test Performance in Adolescent Students with Borderline Intellectual Functioning Compared to Age- and Sex-Matched Controls
Authors: Bauer Julian , Kammermeier Helena , Schwiertz Gerrit , Muehlbauer Thomas
Published: September 2021

The Lower (YBT-LQ) and Upper (YBT-UQ) Quarter Y Balance Test are well established assessment tools for the examination of dynamic balance and shoulder mobility/stability, respectively. However, investigations on YBT-LQ/UQ performance in students with borderline intellectual functioning (BIF) (i.e., intelligence quotient of 70–84 etc.) are lacking. Thus, the aim of the study was to compare YBT-LQ/UQ performance in students with and without BIF. Thirty students with BIF (age: 13.7 ± 1.2 years) and 30 age-/sex-matched students without BIF (age: 13.7 ± 1.3 years) performed the YBT-LQ and/or YBT-UQ. Normalized maximal reach distances (% leg/arm length) per reach direction and the composite score were used as outcome measures. A univariate analysis of variance was conducted to test for significant group differences. Irrespective of limb and reach direction, students with BIF compared to those without BIF showed significantly worse YBT-LQ (p ≤ 0.001–0.031; Cohen’s d = 0.57–1.26) and YBT-UQ (p ≤ 0.001–0.015; Cohen’s d = 0.68–1.52) performance with moderate to large effect sizes. Due to the poorer performance levels of students with BIF, specifically tailored interventions should be developed that have the potential to improve their dynamic balance and shoulder mobility/stability.
The Effect of Ranibizumab Loading Treatment on Vision-Related Quality of Life in Diabetic Macular Edema
Authors: Daldal Hatice , Turkyilmaz Mustafa , Balikoglu Yilmaz Melike , Berberoglu Ufuk
Published: September 2021

Aims: To investigate the changes in vision-related quality of life after a loading dose of three consecutive intravitreal ranibizumab (IVR) injections in patients with unilateral diabetic macular edema (DME). Materials and Methods: Fifty-two eyes of 52 patients who received IVR injections in only one eye with DME were included in our study. The following characteristics of the patients were recorded: gender, education status, marital status, work status, presence of chronic disease. The changes in best-corrected visual acuity (BCVA) and central macular thickness (CMT) were evaluated at baseline (before treatment) and 1 month after the third intravitreal injection (after treatment). Patients were administered the Turkish form of the National Eye Institute 25-Item Visual Functions Questionnaire (NEI VFQ-25 TR). The quality of life scores assessed by the NEI VFQ-25 TR, the BCVA, intraocular pressure (IOP), and CMT measurements were compared at baseline (before treatment) and 1 month after the third intravitreal injection (after treatment). Results: We enrolled 52 patients (25 females, 27 males) in our study; mean age was 64.35 ± 9.26 years. After treatment, BCVA improved significantly (p = 0.001), and macular thickness decreased significantly (p < 0.001). All NEI VFQ-25 TR subscale scores were significantly higher after treatment (p < 0.05). However, no significant correlation was found between the change in BCVA and CMT and the change in NEI VFQ-25 TR subscale and composite scores. The increase in near activities scores was significantly higher in males (p = 0.020) and in the retired group (p = 0.022). There were no significant differences in the changes in NEI VFQ-25 TR subscale and composite scores in relation to educational status. Discussion: Significant improvements in BCVA, macular edema, and vision-related quality of life were found in DME patients who received IVR injections with a loading dose, as shown by the NEI VFQ-25 TR. Interestingly, a significant improvement in quality of life was observed even though the patients could see well with the fellow eye. In conclusion, the NEI VFQ-25 TR is a useful scale to evaluate the changes in visual function and psychosocial characteristics of DME patients after treatment.
Early Diagnosis of Lung Cancer in the United Arab Emirates: Challenges and Strategic Recommendations
Authors: Al-Shamsi Humaid O. , Jaffar Hassan , Mahboub Bassam , Khan Faraz , Albastaki Usama , Hammad Sayed , Zaabi Ashraf Al
Published: September 2021

In the United Arab Emirates (UAE), lung cancer (LC) was the third leading cause of deaths due to cancer in 2017. Around 80% of the patients in the UAE are diagnosed at a late stage, rendering the treatment less effective in improving survival outcomes. Lack of awareness of disease symptomatology, deficient screening initiatives, misdiagnosis, and delayed referral to the specialist are contributing factors for delayed diagnosis. Effective screening at a primary care setting can be crucial for early diagnosis, referral to specialists, and enhancing patient outcomes. It is important to establish screening and referral guidelines through which each suspected case can be identified and provided timely intervention. Although the international screening and referral pathway framework are comprehensive, several regional barriers need to be addressed before they can be adapted at the national level. A group of LC experts from the UAE deliberated on issues like delayed diagnosis of LC and strategic recommendations for overcoming the challenges. The discussion was based on the review of the published evidence, international and regional guidelines for screening and early diagnosis of LC. Herein, we present a guideline, endorsed by the esteemed panel of experts, for aiding early diagnosis and optimizing the management of LC in the UAE.
Free Energy Change during the Formation of Crystalline Contact between Lysozyme Monomers under Different Physical and Chemical Conditions
Authors: Kordonskaya Yuliya , Timofeev Vladimir , Dyakova Yulia , Marchenkova Margarita , Pisarevsky Yury , Kovalchuk Mikhail
Published: September 2021

We use the MM/GBSA method to calculate the free energies of dimer formation by binding two monomers with different combinations of precipitant ions, both embedded in the structure of monomers and in the crystallization solution. It shows that the largest difference in free energy values corresponds to the most accurate dimer model, which considers all precipitant ions in their structure. In addition, it shows that in the absence of precipitant ions in the solution of lysozyme molecules, a monomer is a more energetically favorable state.
Structures of a Phosphoryl Derivative of 4-Allyl-2,4-dihydro-3H-1,2,4-triazole-3-thione: An Illustrative Example of Conformational Polymorphism
Authors: Fedyanin Ivan V. , Samigullina Aida I. , Krutov Ivan A. , Gavrilova Elena L. , Zakharychev Dmitry V.
Published: September 2021

Two polymorphic forms of a conformationally flexible molecule, 5-[(Diphenylphosphoryl)methyl]-4-(prop-2-en-1-yl)-2,4-dihydro-3H-1,2,4-triazole-3-thione, were obtained by crystallization and characterized by X-ray diffraction analysis and differential scanning calorimetry. The relative stability of polymorphic forms was estimated with DFT calculations of crystal structures and isolated molecules. It turns out, that in the first more dense polymorph with higher cohesion energy and crystal lattice energy, the molecule adopts an energetically unfavorable conformation, and forms dimers with lower H-bond strength, as compared to the second polymorph. On the other hand, in the second polymorph, the molecule adopts almost the lowest-energy conformation and forms infinite chains via strong H-bonds. The first form that seems to be more thermodynamically stable at room temperature transforms into the second form via two endothermic phase transitions; the apparent irreversibility of the transition is due to high energy difference between the molecular conformations in crystals.
Management of Acute Demyelinating Attacks in the Pediatric Population: A Swiss Consensus Statement
Authors: Hofer Seline , Bauder Florian , Capone Mori Andrea , Chan Andrew , Dill Patricia , Garcia-Tarodo Stéphanie , Goeggel Simonetti Barbara , Hackenberg Annette , Kalser Judith , Maier Oliver , Schmid Regula , Strozzi Susi , Bigi Sandra ,   on behalf of the “Medico Scientific Advisory Board” of the Swiss Multiple Sclerosis Society
Published: September 2021

Background and methods: Acquired demyelinating syndromes (ADS) encompass distinct entities and occur in approximately 1/100,000 children. While the use of high dose intravenous corticosteroids is well-established, agreement on steroid taper and type of second line therapy is lacking. A comprehensive, unified and standardized treatment approach is crucial in the management of patients with rare diseases. Therefore, this study performed from July 2018 to June 2020 aimed at developing a national consensus on the management of ADS in the pediatric population using the Delphi approach. Consensus was defined as agreement in >75%. Designated Neuropediatricians with an expertise in the management of pediatric neuroinflammatory diseases in all university and cantonal hospitals of Switzerland were included. The response rate was 100%. Results: High-dose i.v. methylprednisolone (20–30 mg/kg/die for 5 days) is the first line treatment irrespective of the distinct entity of the ADS. An oral steroid taper is recommended in acute demyelinating encephalomyelitis (ADEM) and in neuromyelitis optica spectrum disorder (NMO-SD). However, in the latter more in the sense of bridging. The choice of second line treatment depends on the entity of ADS: in optic neuritis (ON) and ADS due to relapsing remitting multiple sclerosis, first line treatment should be repeated, whereas plasma exchange is recommended in NMO-SD, ADEM and transverse myelitis. Conclusions: A national guideline allowing for a more unified approach in the management of pediatric ADS will enhance future research in this field, making data more comparable. The definition of inadequate treatment response to first line therapy remains a challenge and requires future research.
A Personalized Medical Decision Support System Based on Explainable Machine Learning Algorithms and ECC Features: Data from the Real World
Authors: Gu Dongxiao , Zhao Wang , Xie Yi , Wang Xiaoyu , Su Kaixiang , Zolotarev Oleg V.
Published: September 2021

Artificial intelligence can help physicians improve the accuracy of breast cancer diagnosis. However, the effectiveness of AI applications is limited by doctors’ adoption of the results recommended by the personalized medical decision support system. Our primary purpose is to study the impact of external case characteristics (ECC) on the effectiveness of the personalized medical decision support system for breast cancer assisted diagnosis (PMDSS-BCAD) in making accurate recommendations. Therefore, we designed a novel comprehensive framework for case-based reasoning (CBR) that takes the impact of external features of cases into account, made use of the naive Bayes and k-nearest neighbor (KNN) algorithms (CBR-ECC), and developed a PMDSS-BCAD system by using the CBR-ECC model and external features as system components. Under the new case-based reasoning framework, the accuracy of the combined model of naive Bayes and KNN with an optimal K value of 2 is 99.40%. Moreover, in a real hospital scenario, users rated the PMDSS-BCAD system, which takes into account the external characteristics of the case, better than the original personalized system. These results suggest that PMDSS-BCD can not only provide doctors with more personalized and accurate results for auxiliary diagnosis, but also improve doctors’ trust in the results, so as to encourage doctors to adopt the results recommended by the personalized system.
Predict Treatment Response by Magnetic Resonance Diffusion Weighted Imaging: A Preliminary Study on 46 Meningiomas Treated with Proton-Therapy
Authors: Feraco Paola , Scartoni Daniele , Porretti Giulia , Pertile Riccardo , Donner Davide , Picori Lorena , Amelio Dante
Published: September 2021

Objective: a considerable subgroup of meningiomas (MN) exhibit indolent and insidious growth. Strategies to detect earlier treatment responses based on tumour biology rather than on size can be useful. We aimed to characterize therapy-induced changes in the apparent diffusion coefficient (ADC) of MN treated with proton-therapy (PT), determining whether the pre- and early post-treatment ADC values may predict tumour response. Methods: Forty-four subjects with MN treated with PT were retrospectively enrolled. All patients underwent conventional magnetic resonance imaging (MRI) including diffusion-weighted imaging (DWI) at baseline and each 3 months for a follow-up period up to 36 months after the beginning of PT. Mean relative ADC (rADCm) values of 46 MN were measured at each exam. The volume variation percentage (VV) for each MN was calculated. The Wilcoxon test was used to assess the differences in rADCm values between pre-treatment and post-treatment exams. Patients were grouped in terms of VV (threshold −20%). A p < 0.05 was considered statistically significant for all the tests. Results: A significant progressive increase of rADCm values was detected at each time point when compared to baseline rADCm (p < 0.05). Subjects that showed higher pre-treatment rADCm values had no significant volume changes or showed volume increase, while subjects that showed a VV < −20% had significantly lower pre-treatment rADCm values. Higher and earlier rADCm increases (3 months) are related to greater volume reduction. Conclusion: In MN treated with PT, pre-treatment rADCm values and longitudinal rADCm changes may predict treatment response.
Impact of Investment in Tourism Infrastructure Development on Attracting International Visitors: A Nonlinear Panel ARDL Approach Using Vietnam’s Data
Authors: Nguyen Quang Hai
Published: September 2021

Investment in tourism infrastructure development to make destinations and services increasingly attractive is considered a key measure in developing a country’s tourist destinations. This paper investigates the impact of investment in tourism infrastructure components on international visitor attraction using data from Vietnam for the period 1995–2019. The results of analyzing panel data by the nonlinear Autoregressive Distributed Lag (ARDL) approach show that, in the long-run, investing in the three components of tourism infrastructure, namely transport and communications infrastructure, the hotel and restaurant industry, and recreation facilities, has a strong and positive impact on international visitor attraction. In addition, different short-run impacts of the three tourism infrastructure components on the whole market and each major international visitor market are also found.