bluuebunny/crossref_metadata_2025 huggingface dataset:
Supraharmonics Reconstruction Method Based on Blackman Window and Compressed Sensing
Authors: Zhong Fei , Zhang Xiao , Zhu Yangyang , Guan Lining , Jiang Zhihong , Chen Zhe
Published: July 2024

This paper proposes a method that combines window functions with compressed sensing for the detection of ultra-high harmonics in the frequency range of 2–150 kHz. By analyzing the sparsity of the signal, the Bruckmann window function, which is most suitable for the compressed sensing reconstruction process and the characteristics of ultra-high harmonics, is selected. Simulations indicate that, compared to existing methods, the proposed algorithm based on the fusion of the Bruckmann window and compressed sensing achieves a sparser post-observation signal with reduced fluctuations. The robustness and anti-interference capabilities are enhanced, while the harmonic detection accuracy and signal reconstruction performance are significantly improved. The reconstruction error reaches 4.15 × 10−6, the mean squared error percentage (MSE) reaches 4.13 × 10−6, and the signal-to-noise ratio (SNR) is as high as 97.69 dB, marking an increase of 54.11%. This study provides a new theoretical and methodological approach for the analysis and processing of ultra-high harmonics caused by a high proportion of power electronic devices.
Research on Gas Injection Limits and Development Methods of CH4/CO2 Synergistic Displacement in Offshore Fractured Condensate Gas Reservoirs
Authors: Yang Chenxu , Wu Jintao , Wu Haojun , Jiang Yong , Song Xinfei , Guo Ping , Zhang Qixuan , Tian Hao
Published: July 2024

Gas injection for enhanced oil and gas reservoir recovery is a crucial method in offshore Carbon Capture, Utilization, and Storage (CCUS). The B6 buried hill condensate gas reservoir, characterized by high CO2 content, a deficit in natural energy, developed fractures and low-pressure differentials between formation and saturation pressures, requires supplementary formation energy to mitigate retrograde condensation near the wellbore area through gas injection. However, due to the connected fractures, the B6 gas reservoir exhibits strong horizontal and vertical heterogeneity, resulting in severe gas channeling and a futile cycle, which affects the gas injection efficiency at various levels of fracture development. Based on these findings, we conducted gas injection experiments and numerical simulations on fractured cores. A characterization method for oil and gas relative permeability considering dissolution was established. Additionally, the gas injection development boundary for this type of condensate gas reservoir was quantified according to the degree of fracture development, and the gas injection mode of the B6 reservoir was optimized. Research indicates that the presence of fractures leads to the formation of a dominant gas channel; the greater the permeability difference, the poorer the gas injection effect. The permeability gradation (fracture permeability divided by matrix permeability) in the gas injection area should be no higher than 15; gas injection in wells A1 and A2 is likely to achieve a better development effect under the existing well pattern. Moreover, early gas injection timing and pulse gas injection prove beneficial in enhancing the recovery rate of condensate oil. The study offers significant guidance for the development of similar gas reservoirs and for reservoirs with weakly connected fractures; advancing the timing of gas injection can mitigate the retrograde condensation phenomenon, whereas initiating gas injection after depletion may reduce the impact of gas channeling for reservoirs with strongly connected fractures.
Effects of Castor and Corn Biodiesel on Engine Performance and Emissions under Low-Load Conditions
Authors: Lee Keunsang , Cho Haeng Muk
Published: July 2024

Growing concerns over resource depletion and air pollution driven by the rising dependence on fossil fuels necessitate the exploration of alternative energy sources. This study investigates the performance and emission characteristics of a diesel engine fueled by biodiesel blends (B10 and B20) derived from castor and corn feedstocks under low-load conditions (idle and minimal accessory loads). We compare the impact of these biofuels on engine power, fuel consumption, and exhaust emissions relative to conventional diesel, particularly in scenarios mimicking real-world traffic congestion and vehicle stops. The findings suggest that biodiesel offers environmental benefits by reducing harmful pollutants like carbon monoxide (CO) and particulate matter (PM) during engine idling and low-load operation. However, replacing diesel with biodiesel requires further research to address potential drawbacks like increased NOx emissions and lower thermal efficiency. While a higher fuel consumption with biodiesel may occur due to its lower calorific value, the overall benefit of reduced contaminant emissions makes it a promising alternative fuel.
Economic Feasibility of LNG Business: An Integrated Model and Case Study Analysis
Authors: Zhang Jin , Yin Xiuling , Lei Zhanxiang , Wang Jianjun , Fan Zifei , Liu Shenaoyi
Published: July 2024

Liquefied natural gas (LNG), recognized as the fossil fuel with the lowest carbon emission intensity, is a crucial transitional energy source in the global shift towards low-carbon energy. As the natural gas industry undergoes rapid expansion, the complexity of investment business models has increased significantly. Optimizing the combination of various segments within the value chain has become standard practice, making it essential to control risks and enhance economic benefits in these multifaceted scenarios. This paper introduces an integrated economic model encompassing upstream, liquefaction, shipping, regasification, and consumption, suitable for both upstream and downstream integration. The model offers a comprehensive analysis of the primary business models and key factors across each segment of the value chain. By constructing a robust economic evaluation framework, the study aims to provide a holistic approach to understanding the economic feasibility of LNG projects. Two detailed case studies are conducted to demonstrate the application and effectiveness of the proposed model, highlighting its capability to guide investment decisions, support risk management, and optimize asset portfolios. The integrated economic model developed in this study serves as a valuable tool for stakeholders in the LNG industry. It not only facilitates informed investment decision-making but also enhances the strategic management of risks and resources. By leveraging this model, investors and managers can better navigate the complexities of the LNG business, ensuring sustainable and economically viable operations.
Land Cover Dynamics and Environmental Implications in Türkiye’s Riparian Zones: A Comprehensive Seven-Year Study
Authors: Akturk Emre , Guney Kerim , Aydin Mirac , Yildiz Faruk , Kaya Ozkan
Published: July 2024

This study examines the Riparian Buffer Zones (RBZs) in Türkiye, analyzing land cover changes from 2017 to 2023 to assess their effectiveness in ecological conservation and water resource management. The research employs Esri-Sentinel land cover data, Shuttle Radar Topography Mission digital elevation models, and RiverATLAS data to identify and delineate RBZs, classify land cover types, and evaluate trends and changes over seven years. The findings indicate that 44.8% of RBZs are covered by ‘Rangelands’, 32.2% by ‘Crops’, and 16.1% by ‘Trees’ classes. The study notes a considerable increase in built areas and agricultural fields, which may indicate ongoing urban expansion and its potential impact on the effectiveness of RBZs. Despite regulatory efforts, the substantial presence of agriculture within RBZs indicates a need for more robust environmental protection policies. The results indicate a pressing need for a policy reassessment to enhance forest cover within RBZs and reduce agricultural encroachment. This would improve water quality and facilitate the achievement of sustainability goals numbered 2, 3, 6, 11, 14 and 15. The findings thus call for a strategic enhancement of RBZ management to better protect water resources against the adverse effects of agricultural and urban activities.
Dynamic SNR, Spectral Efficiency, and Rate Characterization in 5G/6G mmWave/sub-THz Systems with Macro- and Micro-Mobilities
Authors: Ostrikova Darya , Golos Elizaveta , Beschastnyi Vitalii , Machnev Egor , Gaidamaka Yuliya , Samouylov Konstantin
Published: July 2024

The performance of 5G/6G cellular systems operating in millimeter wave (mmWave, 30–100 GHz) and sub-terahertz (sub-THz, 100–300 GHz) bands is conventionally assessed by utilizing the static distributions of user locations. The rationale is that the use of the beam tracking procedure allows for keeping the beams of a base station (BS) and user equipment (UE) aligned at all times. However, by introducing 3GPP Reduced Capability (RedCap) UEs utilizing the Radio Resource Management (RRM) Relaxation procedure, this may no longer be the case, as UEs are allowed to skip synchronization signal blocks (SSB) to improve energy efficiency. Thus, to characterize the performance of such UEs, methods explicitly accounting for UE mobility are needed. In this paper, we will utilize the tools of the stochastic geometry and random walk theory to derive signal-to-noise ratio (SNR), spectral efficiency, and rate as an explicit function of time by accounting for mmWave/sub-THZ specifics, including realistic directional antenna radiation patterns and micro- and macro-mobilities causing dynamic antenna misalignment. Different from other studies in the field that consider time-averaged performance measures, these metrics are obtained as an explicit function of time. Our numerical results illustrate that the macro-mobility specifies the overall trend of the time-dependent spectral efficiency, while local dynamics at 1–3 s scales are mainly governed by micro-mobility. The difference between spectral efficiency corresponding to perfectly synchronized UE and BS antennas and time-dependent spectral efficiency in a completely desynchronized system is rather negligible for realistic cell coverages and stays within approximately 5–10% for a wide range of system parameters. These conclusions are not affected by the utilized antenna array at the BS side. However, accounting for realistic radiation patterns is critical for a time-dependent performance analysis of 5G/6G mmWave/sub-THz systems.
Chemical Characterization, Stability and Sensory Evaluation of Sicilian Extra Virgin Olive Oils: Healthiness Evidence at Nose Reach
Authors: Lino Claudia , Bongiorno David , Pitonzo Rosa , Indelicato Serena , Barbera Manfredi , Di Gregorio Gabriella , Pane Domenico , Avellone Giuseppe
Published: July 2024

The aim of this study was to assess the nutraceutical qualities of extra virgin olive oil (EVOO) samples obtained from three Sicilian olive cultivars: Nocellara, Biancolilla, and Cerasuola. We also evidenced the relationship among biophenols, base parameters and panel test scores, and evaluated the stability of the biophenols in EVOO. The assessment also took into consideration variations in olive harvesting periods and the influence of four different milling methods. A statistical analysis of the collected data revealed that the cultivar and harvesting period were the primary factors influencing the bio-phenol content, while the milling methods employed did not significantly affect the levels of biophenols in the oils. The panel test results were also illuminating as they were strongly related to the cultivar and polyphenol content. Following the criteria outlined in EC Regulation 432/2012, we selected three samples, each representing one of the cultivars, which exhibited the highest bio-phenol content to evaluate the biophenol stability during a time span of 16 months.
Impact of Germination on the Edible Quality and Nutritional Properties of Brown Rice Noodles
Authors: Chen Ruiyun , Yan Xudong , Cai Mingxi , Cai Jiamei , Dai Taotao , Liu Yunfei , Wu Jianyong
Published: July 2024

Brown rice noodles are increasingly favored by consumers for their health benefits; however, their development is hindered by their poor edible qualities. The effect of germination on the cooking, textural, organoleptic and nutritional qualities of brown rice pasta was investigated. In comparison to ungerminated brown rice noodles, germination resulted in a shorter cooking time, reduced cooking losses, and decreased hardness and adhesion of noodles as well as reduced bitter taste. These changes can be attributed to germination altering the basic composition of brown rice. Meanwhile, the contents of γ-aminobutyric acid, free phenolic acid, and bound phenolic acid increased by 53.43%, 21.71%, and 7.14%, respectively, while the content of resistant starch de-creased by 21.55%. Sprouting is a promising strategy for improving the edible quality and nutritional properties of brown rice noodles.
Endogenous Proteases in Sea Cucumber (Apostichopus japonicas): Deterioration and Prevention during Handling, Processing, and Preservation
Authors: Fan Xinru , Wu Ke , Tian Xiuhui , Benjakul Soottawat , Li Ying , Sang Xue , Zhao Qiancheng , Zhang Jian
Published: July 2024

The sea cucumber is an essential nutrient source and a significant economic marine resource associated with successful aquaculture. However, sea cucumbers are highly susceptible to autolysis induced by endogenous protease after postmortem, and the phenomenon of body wall “melting” occurs, which seriously affects the food quality of products and the degree of acceptance by consumers. To satisfy the growing demand for fresh or processed sea cucumbers, we must clarify the autolysis mechanism of sea cucumbers and the methods to achieve autolysis regulation. In this paper, the factors leading to the quality deterioration and texture softening of sea cucumbers are reviewed, with emphasis on enzymatic characteristics, the autolysis mechanism, the effects of autolysis on the physicochemical properties of the body wall of the sea cucumber, and the development of potential natural protease inhibitors. We aim to provide some reference in future preservation and processing processes for sea cucumbers, promote new processing and preservation technologies, and advance the sea cucumber industry’s development.
The Role of Nondigestible Oligosaccharides in Alleviating Human Chronic Diseases by Regulating the Gut Microbiota: A Review
Authors: Yuan Meiyu , Zhang Zhongwei , Liu Tongying , Feng Hua , Liu Yuhuan , Chen Kai
Published: July 2024

The gut has been a focus of chronic disease research. The gut microbiota produces metabolites that act as signaling molecules and substrates, closely influencing host health. Nondigestible oligosaccharides (NDOs), as a common dietary fiber, play an important role in regulating the structure and function of the gut microbiota. Their mechanism of action is mainly attributed to providing a carbon source as specific probiotics, producing related metabolites, and regulating the gut microbial community. However, due to the selective utilization of oligosaccharides, some factors, such as the type and structure of oligosaccharides, have different impacts on the composition of microbial populations and the production of metabolites in the colon ecosystem. This review systematically describes the key factors influencing the selective utilization of oligosaccharides by microorganisms and elaborates how oligosaccharides affect the host’s immune system, inflammation levels, and energy metabolism by regulating microbial diversity and metabolic function, which in turn affects the onset and progress of chronic diseases, especially diabetes, obesity, depression, intestinal inflammatory diseases, and constipation. In this review, we re-examine the interaction mechanisms between the gut microbiota and its associated metabolites and diseases, and we explore new strategies for promoting human health and combating chronic diseases through dietary interventions.
Elaboration and Characterization of Novel Kombucha Drinks Based on Truffles (Tuber melanosporum and Tuber aestivum) with Interesting Aromatic and Compositional Profiles
Authors: Morales Diego , de la Fuente-Nieto Laura , Marco Pedro , Tejedor-Calvo Eva
Published: July 2024

The organoleptic and bioactive properties of truffles place these fungi as interesting materials for use in the of design functional foods based on fruiting bodies outside commercial standards. Moreover, kombucha beverages have become more popular in the Western world, leading to novel drinks using alternative substrates instead of tea leaves. In this work, two truffle species (Tuber melanosporum, TMEL; Tuber aestivum, TAES) and three different symbiotic consortia of bacteria and yeasts (SCOBYs: SC1, SC2, and SC3) were tested. Fermentation (21 days) was monitored in terms of physicochemical (pH, viscosity), biochemical (total carbohydrates, alcohol, soluble proteins, phenolic compounds), and sensory attributes (volatile organic compounds, VOCs). The obtained pH ranges were adequate, alcohol levels were undetectable or very low, and sugar content was lower than in traditional kombuchas or other beverages. In most cases, the usual bottling time could be applied (7–10 days), although longer fermentations are recommended (14 days) to reach higher protein and phenolic compounds contents. Truffle kombuchas produced up to 51 volatile organic compounds (alcohols, acids, esters, ketones, and aldehydes, among others), with TMEL showing a more complex profile than TAES. During the first week, acidic compound production was observed, especially acetic acid. Similar behavior in the VOC profile was reported with different SCOBYs.
CrossMP: Enabling Cross-Modality Translation between Single-Cell RNA-Seq and Single-Cell ATAC-Seq through Web-Based Portal
Authors: Lyu Zhen , Dahal Sabin , Zeng Shuai , Wang Juexin , Xu Dong , Joshi Trupti
Published: July 2024

In recent years, there has been a growing interest in profiling multiomic modalities within individual cells simultaneously. One such example is integrating combined single-cell RNA sequencing (scRNA-seq) data and single-cell transposase-accessible chromatin sequencing (scATAC-seq) data. Integrated analysis of diverse modalities has helped researchers make more accurate predictions and gain a more comprehensive understanding than with single-modality analysis. However, generating such multimodal data is technically challenging and expensive, leading to limited availability of single-cell co-assay data. Here, we propose a model for cross-modal prediction between the transcriptome and chromatin profiles in single cells. Our model is based on a deep neural network architecture that learns the latent representations from the source modality and then predicts the target modality. It demonstrates reliable performance in accurately translating between these modalities across multiple paired human scATAC-seq and scRNA-seq datasets. Additionally, we developed CrossMP, a web-based portal allowing researchers to upload their single-cell modality data through an interactive web interface and predict the other type of modality data, using high-performance computing resources plugged at the backend.
The Development of a Fluorescent Microsatellite Marker Assay for the Pitaya Canker Pathogen (Neoscytalidium dimidiatum)
Authors: Li Rui , Li Xi , Tang Jingcheng , Xie Changping , Wang Jianan
Published: July 2024

Pitaya canker, caused by Neoscytalidium dimidiatum, is a destructive disease that significantly threatens the safety of the pitaya industry. The authors of previous studies have mainly focused on its biological characteristics and chemical control. However, there are no molecular markers available thus far that can be used for the population genetics study of this pathogen. In the present study, a draft genome of N. dimidiatum with a total length of 41.46 MB was assembled in which 9863 coding genes were predicted and annotated. In particular, the microsatellite sequences in the draft genome were investigated. To improve the successful screening rate of potentially polymorphic microsatellite makers, another five N. dimidiatum isolates were resequenced and assembled. A total of eight pairs of polymorphic microsatellite primers were screened out based on the polymorphic microsatellite loci after investigating the sequencing and resequencing assemblies of the six isolates. A total of thirteen representative isolates sampled from different pitaya plantations were genotyped in order to validate the polymorphism of the resulting eight markers. The results indicated that these markers were able to distinguish the isolates well. Lastly, a neighbor-joining tree of 35 isolates, sampled from different pitaya plantations located in different regions, was constructed according to the genotypes of the eight molecular markers. The developed tree indicated that these molecular markers had sufficient genotyping capabilities for our test panel of isolates. In summary, we developed a set of polymorphic microsatellite markers in the following study that can effectively genotype and distinguish N. dimidiatum isolates and be utilized in the population genetics study of N. dimidiatum.
Does Sunlight Affect the Quality for Purposes of DNA Analysis of Blood Stain Evidence Collected from Different Surfaces?
Authors: Sliskovic Livia , Milos Ivana , Zecic Antonia , Kuret Sendi , Sutlovic Davorka
Published: July 2024

The aim of this study was to investigate the effect of sunlight on the degradation of DNA samples taken from blood stains from different types of surfaces. A blood sample obtained from a single male donor was placed on seven different surfaces (galvanized sheet, iron rod, newspaper, white printer paper, glass, soil, and ceramic panel). Samples were kept, during a 4-week summer period, in a room, but next to an open window. Every 7 days, 1 mm2 of blood sample was collected from each substrate and stored in labeled tube for later analysis. DNA was extracted with the Chelex method, amplified using AmpFISTRTM MinifilerTM Plus Amplification Kit, and quantified using a QuantifilerTM Human DNA Quantification kit. After 7 days of sun exposure, the highest DNA concentration was determined to be from the sample from a galvanized sheet stain, followed by, in order of decreasing concentration, the ceramic panel, glass, newspaper, iron rod, and white printer paper surface. As expected, the DNA concentration from all samples decreased as the sunlight exposure time progressed. The results obtained after the amplification in the MiniFilerTM system were in correlation with the DNA concentrations measured by the qPCR method for all samples, except for the glass, soil, and white printer paper samples. The obtained data show that DNA degradation is correlated to the length of sunlight exposure and to the type of surface the samples are collected from. A negative qPCR result does not mean negative PCR amplification in the STR system; therefore, both methods should be applied when analyzing forensic samples collected from trace evidence.
Pathway-Based Mendelian Randomization for Pre-Infection IL-6 Levels Highlights Its Role in Coronavirus Disease
Authors: Kamali Zoha , Esmaeil Nafiseh , Thio Chris H. L. , Vaez Ahmad , Snieder Harold
Published: July 2024

Objectives: Interleukin 6 (IL-6) levels at hospital admission have been suggested for disease prognosis, and IL-6 antagonists have been suggested for the treatment of patients with severe COVID-19. However, less is known about the relationship between pre-COVID-19 IL-6 levels and the risk of severe COVID-19. To fill in this gap, here we extensively investigated the association of genetically instrumented IL-6 pathway components with the risk of severe COVID-19. Methods: We used a two-sample Mendelian randomization study design and retrieved genetic instruments for blood biomarkers of IL-6 activation, including IL-6, soluble IL-6 receptor, IL-6 signal transducer, and CRP, from respective large available GWASs. To establish associations of these instruments with COVID-19 outcomes, we used data from the Host Genetics Initiative and GenOMICC studies. Results: Our analyses revealed inverse associations of genetically instrumented levels of IL-6 and its soluble receptor with the risk of developing severe disease (OR = 0.60 and 0.94, respectively). They also demonstrated a positive association of severe disease with the soluble signal transducer level (OR = 1.13). Only IL-6 associations with severe COVID-19 outcomes reached the significance threshold corrected for multiple testing (p < 0.003; with COVID-19 hospitalization and critical illness). Conclusions: These potential causal relationships for pre-COVID-19 IL-6 levels with the risk of developing severe symptoms provide opportunities for further evaluation of these factors as prognostic/preventive markers of severe COVID-19. Further studies will need to clarify whether the higher risk for a severe disease course with lower baseline IL-6 levels may also extend to other infectious diseases.
Mechanisms and Future Research Perspectives on Mitochondrial Diseases Associated with Isoleucyl-tRNA Synthetase Gene Mutations
Authors: Watanabe Masaki , Sasaki Nobuya
Published: July 2024

Aminoacyl-tRNA synthetases are essential enzymes for the accurate translation of genetic information. IARS1 and IARS2 are isoleucyl-tRNA synthetases functioning in the cytoplasm and mitochondria, respectively, with genetic mutations in these enzymes causing diverse clinical phenotypes in specific organs and tissues. Mutations in IARS1 and IARS2 have recently been linked to mitochondrial diseases. This review aims to explore the relationship between IARS1 and IARS2 and these diseases, providing a comprehensive overview of their association with mitochondrial diseases. Mutations in IARS1 cause weak calf syndrome in cattle and mitochondrial diseases in humans, leading to growth retardation and liver dysfunction. Mutations in IARS2 are associated with Leigh syndrome, craniosynostosis and abnormal genitalia syndrome. Future research is expected to involve genetic analysis of a larger number of patients, identifying new mutations in IARS1 and IARS2, and elucidating their impact on mitochondrial function. Additionally, genetically modified mice and the corresponding phenotypic analysis will serve as powerful tools for understanding the functions of these gene products and unraveling disease mechanisms. This will likely promote the development of new therapies and preventive measures.
Mealworm Larvae Frass Exhibits a Plant Biostimulant Effect on Lettuce, Boosting Productivity beyond Just Nutrient Release or Improved Soil Properties
Authors: Foughar Meroua , Arrobas Margarida , Rodrigues Manuel Ângelo
Published: July 2024

There is a need for alternatives or complements to synthetic fertilizers to enhance agricultural sustainability. Applying organic amendments can play a significant role in this. Insect droppings show high potential, though studies evaluating their agronomic value have only recently begun to emerge. This study compared black soldier fly (Hermetia illucens L.) and mealworm (Tenebrio molitor L.) larvae frass with another organic amendment (Nutrimais) derived from composting forestry, agro-industrial, and domestic waste. The experiment also included ammonium nitrate at two rates [the same as the organic amendments, 50 kg ha−1 nitrogen (N) (FullR), and half that rate (HalfR)] and an unfertilized control. The study spanned two growth cycles of lettuce (Lactuca sativa L.) grown in pots, followed by unfertilized oats (Avena sativa L.) to assess the residual effects of the fertilizing treatments. Mealworm larvae frass mineralized rapidly, with an apparent N recovery of 37.4% over the two lettuce growth cycles, indicating its high availability to soil heterotrophic microorganisms. The average dry matter yield (DMY) of lettuce was the highest among all treatments (12.8 and 9.8 g plant−1 in the first and second lettuce cycles), even compared to the FullR treatment (12.2 and 7.8 g plant−1), though without significant differences. Although mealworm larvae frass exhibited a high mineralization rate, the DMY cannot be attributed solely to N supply, as plants in the FullR treatment showed better N nutritional status. Mealworm larvae frass provided strong evidence of a plant biostimulant effect, not explained by the variables measured in this study. Black soldier fly larvae frass exhibited typical behavior of a moderately reactive organic amendment, while Nutrimais showed low reactivity, with a near-neutral mineralization/immobilization balance. The results suggest mealworm larvae frass is recommended for early maturing vegetable crops, whereas Nutrimais appears more suitable for perennial crops with low short-term nutrient requirements.
Yield and Heat Unit Requirements for Several Citrus Cultivars over Several Seasons in Egypt
Authors: Abdel-Sattar Mahmoud , Al-Obeed Rashid S. , Aboukarima Abdulwahed M.
Published: July 2024

Citrus cultivars have various temperature needs for development and output at different times from flowering to harvesting, making climate one of the numerous factors that affect citrus’ productivity and quality. In this study, the yield and heat unit requirements for several commercial citrus species over several seasons from 2010/2011 to 2021/2022 in Egypt were investigated. For this investigation, the time of flowering and the time of fruit harvesting were recorded. However, the required heat units from flowering to fruit harvesting were calculated based on daily records of air temperature, taking into account that all results below zero (negative results) are not used and all maximum air temperatures above 35.0 °C (≥35.1) are changed to 35.0 °C. In addition, the base air temperature of 13.0 °C was utilized for determining the required heat units. The results showed that in the experimental area, the overall mean of minimum air temperature, maximum air temperature, air relative humidity, and precipitation during the experimental periods had values of 15.2 °C, 28.70 °C, 59.3%, and 0.3 mm/day, respectively. Moreover, the lowest number of days required from flowering to fruit harvesting was observed to be 290.0 days for Fremont mandarin. Furthermore, the highest number of days required from flowering to fruit harvesting was observed to be 482 and 440 days, respectively, for Bearss Seedless lime and Valencia orange. Our study has highlighted a notable diversity among the investigated citrus cultivars, particularly highlighting specific cultivars that possess high yield. The cultivar that shows the greatest yield over the investigated seasons of the study was Valencia orange with 147.6 kg/tree. Moreover, the average values of the heat unit requirements for Washington Navel orange, Valencia orange, Murcott mandarin, Fremont mandarin, and Bearss Seedless lime were 3112.7, 3628.3, 3221.9, 3027.6, and 4398.4 °C day, respectively. This is the first report regarding the heat unit’s determination for several citrus cultivars grown in Egypt, and we expect this research will provide a new awareness in accepting and discovering novel locations where citrus cultivars can be positively developed in Egypt. It will also prove to be a source of basic information for the development of the citrus crop model.
Visual Analyses of Hot Spots and Frontiers in Zanthoxylum planispinum Research Based on CiteSpace
Authors: Yang Shunsong , Guo Youyan , Yang Guangguang , Yu Yanghua
Published: July 2024

Zanthoxylum planispinum is a type of plant with homologous properties in medicine and food, making it well-loved in China. To explore the development of the Z. planispinum field over the past 20 years, its research hotspots and frontier trends were analyzed. This study conducted database-based visualization analyses and knowledge graph analyses using CiteSpace software with data concerning Z. planispinum published in the Chinese National Knowledge Infrastructure and Web of Science databases between 2003 and 2023. Over the last 20 years, the number of Chinese and English publications on Z. planispinum has shown increasing trends. The foci of this research were mainly germplasm resources, cultivation management, seed oil extraction technology, flavonoid extraction technology, and correlation analyses of antioxidant activities. The main research frontiers included the unified standard naming and adaptation mechanisms of Z. germplasm resources, orientation cultivation, functional component extraction, processing technology research and development, and industrial chain construction. The results provide a scientific reference for the high-quality development of the Z. planispinum industry.
Physiological, Cellular, and Transcriptomic Analyses Provide Insights into the Tolerance Response of Arundo donax to Waterlogging Stress
Authors: Wu Dandan , Tian Zhaoran , Guo Jialin , Xie Zhengqing , Tian Baoming , Liu Ziqi , Chen Weiwei , Cao Gangqiang , Zhang Luyue , Yang Tian , Wei Fang , Shi Gongyao
Published: July 2024

Arundo donax is widely used as an ornamental plant in landscape gardening because of its adaptability to varying degrees of waterlogged conditions. However, to date, little information is available about the adaptive mechanism of A. donax under waterlogging stress. The results showed that long-term mild waterlogging efficiently induced the formation of adventitious roots (ARs) and further promoted root aerenchyma development, and that the activity of antioxidant enzymes (SOD, POD, and CAT) in Ars also was greatly enhanced after waterlogging. At the transcriptomic level, the expression of genes related to apoptosis, the regulation of cell division, ethylene biosynthesis, alginate synthesis, auxin signaling pathways, and anaerobic respiration was mostly up-regulated after the occurrence of waterlogging stress but genes involved in the abscisic acid signaling pathways were partly down-regulated, which indicated a preferential and favorable transcriptional response in regulating adventitious root development. Taken together, this study definitely advances our knowledge of the morphological, physiological, and transcriptomic responses of A. donax under waterlogging stress and sheds new lights on its adaptive mechanisms.
Application of Ultrafine Bubble Technology for Reducing Sodium Metabisulfite Concentration in Preserving Trimmed Coconuts
Authors: Imsabai Wachiraya , Pathomaim Supat , Jarussophon Suwatchai
Published: July 2024

The export of trimmed coconuts necessitates controlling microbial growth and browning, often achieved through the use of sodium metabisulfite (SMS). However, SMS can elicit allergic reactions in operators. To address this concern, ultrafine bubble (UFBs) technology was applied to reduce the SMS concentration. Trimmed coconuts were dipped in either a 1.5% SMS solution or a combination of 1.5% SMS with UFBs (1.5% SMS-UFBs) and compared to coconuts dipped or not dipped in a 3% SMS solution. All treated coconuts were then wrapped with polyvinyl chloride (PVC) film and stored at 2–4 °C for 2 months, followed by transfer to storage at 8–10 °C for an additional 14 days. The results indicated that halving the SMS concentration, with or without UFB application, effectively controlled microbial growth and browning, comparable to using 3% SMS. No contamination of E. coli or Salmonella spp. was detected. The mesocarp whiteness, browning index, polyphenol oxidase (PPO) activity, and total phenolic content of coconuts treated with 1.5% SMS or 1.5% SMS-UFBs did not differ significantly from those dipped in 3% SMS solution (p > 0.05). Similarly, the quality of coconut water and coconut meat in SMS or SMS-UFB treatments did not show significant differences. In dry seasons, using 1.5% SMS with or without UFBs yielded comparable results to those obtained using 3% SMS. However, in wet seasons, the synergistic effects of UFBs on reducing microbial growth incidence were observed, similar to the impact achieved with 3% SMS, whereas 1.5% SMS alone did not inhibit microbial growth. Overall, UFB technology shows promise in reducing SMS concentration by 50% for trimmed young coconuts throughout the year.
Effects of Ripening Phase and Cultivar under Sustainable Management on Fruit Quality and Antioxidants of Sweet Cherry
Authors: Mineață Iulia , Murariu Otilia Cristina , Sîrbu Sorina , Tallarita Alessio Vincenzo , Caruso Gianluca , Jităreanu Carmen Doina
Published: July 2024

Sweet cherry grown under sustainable management produces highly valuable fruits, whose quality shows important biochemical and morphological changes during ripening. Research was carried out in Iasi (Romania), with the aim to assess the quality characteristics of the sweet cherry fruits of three cultivars (Van, Andreiaș, Margonia), grown in an inner or outer position inside the tree crown, at the pre-ripening or full ripeness phase. In 2022, the colour component a* showed higher values in cv. Van and Andreias red fruits and in an inner position, whereas the components L* and b* at the full ripeness phase were highest in cv. Margonia. The dry matter and total soluble solids contents increased from the pre-ripening to the full ripeness phase and were highest in cv. Van sweet cherry fruits; the DM of fruit from the outer part of crown was higher than that of fruit from the inner part at the pre-ripening phase. The content of phenolics was the highest in cv. Margonia fruits at the pre-ripening stage and in cv. Van at the full ripeness phase and higher in the inner tree crown zones. The cultivar Margonia generally showed the highest vitamin C content in both years and development phases. The yellow fruit cv. Margonia mostly showed the highest values of chlorophyll a and b. The fruit’s content of carotene, lycopene, and anthocyanins was generally the highest in the red fruits of cv. Andreias. The examined sweet cherry cultivars showed a high variability in fruit nutritional quality and proved to be a rich source of bioactive compounds with antioxidant potential.
The Participatory Approach in Healthcare Establishments as a Specific French Organizational Model at Hospital Department Level to Prevent Burnout among Caregivers: What Are the Perceptions of Its Implementation and Its Potential Contributions by These Caregivers?
Authors: Arnaudeau Sophie , Nickum Marion , Fouquereau Evelyne , Chevalier Séverine , Gillet Nicolas , Mokounkolo René , Lejeune Julien , Seizeur Romuald , Colombat Philippe , Jeoffrion Christine
Published: July 2024

(1) Background: Manifestations of burnout are regularly observed in the healthcare population. The participatory approach (PA) is a specific organization in the French health service aimed at preserving and improving the psychological health of these staff at work. The main objective of this study was to explore with healthcare professionals their perceptions of the effectiveness of the four PA components (multi-professional team meetings, in-service training, team support meetings and the project approach) implemented to date within French hospital departments, the methods of their implementation and the potential contributions of such an approach to their quality of working life and working conditions (QWLWC), and the quality of care provided. (2) Methods: Semi-structured qualitative interviews were conducted with 21 healthcare professionals in French hospital departments between March and April 2021. After they were recorded, the collected data was transcribed in full and subjected to thematic analysis. (3) Results: According to care providers, PA is only partially deployed in these departments today. Nevertheless, it is helping to develop multi-professional communication, and improves the quality of life at work as well as quality of care. (4) Conclusions: In the light of these results, the creation of a tool for the large-scale evaluation of PA implementation in hospitals emerges as essential, as its deployment in all hospital departments could help reduce the suffering of care professionals. In addition, a better articulation between the concepts of Magnet Hospitals and those of PA would prove heuristically promising.
Customer Healthcare Complaints in Brazil Are Seldom about Medical Errors
Authors: Ryngelblum Arnaldo , Šostar Marko , Andrlić Berislav
Published: July 2024

This study reviewed different country studies and noted that complaints in Brazil are more concentrated in complaints about being attended to and receiving access to services, rather than about clinical quality and safety issues. This paper explores the possible explanations for these differences based on the institutional logics theory and which logics actors privilege, and how they may play out in the healthcare field. To accomplish this undertaking, this study makes use of the healthcare complaint categorization developed by Reader and colleagues, which has been used by various studies. Next, a set of studies about healthcare complaints in different countries was examined to analyze the issues most common in the complaints and compare this information with the Brazilian data. This study identified three explanations why complaints about medical errors seldom occur. One group of studies highlights the hardships of local health systems. Another focuses on patient behavior. Finally, the third kind focuses on the issue of power to determine health orientation. The studies about a lack of resources do not directly explain why fewer complaints about clinical quality occur, thus helping to stress the management issues. Patient behavior studies indicate that patients may be afraid to point out medical errors or may be unaware of the procedures of how to do so, suggesting that family logic is left out of the decisions in the field. The third group of work highlights the prominence of the medical professional logic, both in terms of regulation and medical exercise.
Riding the Wave of Ambivalence in Cell Biology
Authors: Emanuele Sonia , Giuliano Michela
Published: July 2024

Increasing evidence clearly shows that most functional molecules in the cell exert a dual role depending on the specific interactive context, biochemical pathway, or subcellular localization [...]
Agave Wilt Susceptibility by Reduction of Free Hexoses in Root Tissue of Agave tequilana Weber var. azul Commercial Plants in the Fructan Accumulation Process
Authors: Mantilla-Blandon Rodrigo Guillermo , Mancilla-Margalli Norma Alejandra , Molina-Montes Joaquín Adolfo , Uvalle-Bueno Jaime Xavier , Avila-Miranda Martín Eduardo
Published: July 2024

Agave tequilana stems store fructan polymers, the main carbon source for tequila production. This crop takes six or more years for industrial maturity. In conducive conditions, agave wilt disease increases the incidence of dead plants after the fourth year. Plant susceptibility induced for limited photosynthates for defense is recognized in many crops and is known as “sink-induced loss of resistance”. To establish whether A. tequilana is more prone to agave wilt as it ages, because the reduction of water-soluble carbohydrates in roots, as a consequence of greater assembly of highly polymerized fructans, were quantified roots sucrose, fructose, and glucose, as well as fructans in stems of agave plants of different ages. The damage induced by inoculation with Fusarium solani or F. oxysporum in the roots or xylem bundles, respectively, was recorded. As the agave plant accumulated fructans in the stem as the main sink, the amount of these hexoses diminished in the roots of older plants, and root rot severity increased when plants were inoculated with F. solani, as evidence of more susceptibility. This knowledge could help to structure disease management that reduces the dispersion of agave wilt, dead plants, and economic losses at the end of agave’s long crop cycle.
Cyclo(l-Pro-l-Tyr) Isolated from the Human Skin Commensal Corynebacterium tuberculostearicum Inhibits Tyrosinase
Authors: Sekino Yuika , Yamamoto Ikuya , Watanabe Masahiro , Kuramochi Kouji , Furuyama Yuuki
Published: July 2024

Melanin is produced by melanocytes to protect human skin from harmful ultraviolet radiation. During skin cell renewal, melanin and dead skin cells are disposed of. However, prolonged exposure to ultraviolet rays or aging can disturb this cycle, leading to skin hyperpigmentation due to melanin accumulation. Tyrosinase is a crucial enzyme involved in melanin biosynthesis. Although various compounds, including tyrosine inhibitors, that counteract melanin accumulation have been reported, some, such as hydroquinone, are toxic and can cause vitiligo. Meanwhile, the skin is the largest organ and the outermost layer of the immune system, containing a diverse range of bacteria that produce low-toxicity compounds. In the current study, we aim to identify metabolites produced by skin microbiota that inhibit tyrosinase. Specifically, mushroom tyrosinase served as the study model. Following commensal skin bacteria screening, Corynebacterium tuberculostearicum was found to inhibit tyrosinase activity. The active compound was cyclo(l-Pro-l-Tyr); commercially available cyclo(l-Pro-l-Tyr) also exhibited inhibitory activity. Docking simulations suggested that cyclo(l-Pro-l-Tyr) binds to the substrate-binding site of mushroom tyrosinase, obstructing the substrate pocket and preventing its activity. Hence, cyclo(l-Pro-l-Tyr) might have potential applications as a cosmetic agent and food additive.
Current Knowledge about Nonclassical Monocytes in Patients with Multiple Sclerosis, a Systematic Review
Authors: Arneth Borros
Published: July 2024

Monocytes play a critical role in the initiation and progression of multiple sclerosis (MS). Recent research indicates the importance of considering the roles of monocytes in the management of MS and the development of effective interventions. This systematic review examined published research on the roles of nonclassical monocytes in MS and how they influence disease management. Reputable databases, such as PubMed, EMBASE, Cochrane, and Google Scholar, were searched for relevant studies on the influence of monocytes on MS. The search focused on studies on humans and patients with experimental autoimmune encephalomyelitis (EAE) published between 2014 and 2024 to provide insights into the study topic. Fourteen articles that examined the role of monocytes in MS were identified; the findings reported in these articles revealed that nonclassical monocytes could act as MS biomarkers, aid in the development of therapeutic interventions, reveal disease pathology, and improve approaches for monitoring disease progression. This review provides support for the consideration of monocytes when researching effective diagnostics, therapeutic interventions, and procedures for managing MS pathophysiology. These findings may guide future research aimed at gaining further insights into the role of monocytes in MS.