bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of ZnO-Nd Nano-Photocatalyst for the Reactive Red 198 Dye Decolorization in the Falling-Film Photocatalytic Reactor
Authors: Biglar Fatemeh , Talaiekhozani Amirreza , Aminsharei Farham , Park Junboum , Barghi Anahita , Rezania Shahabaldin
Published: October 2021

A large amount of Reactive red 198 (RR198) is released yearly into the environment. RR198 is toxic for human and aquatic creatures; therefore, it should be removed from wastewater before releasing into the environment. In this study, the nano ZnO-Nd -photo-catalyst for the first time was synthesized by the combustion method. First, the physical characteristics of the generated nano photocatalyst were evaluated using FESEM, XRD, Bandgap calculation, and FTIR analysis. Then, the ZnO-Nd nano-photocatalyst was suspended into the contaminated water with RR198 dye in a falling-film photocatalytic reactor. The effects of parameters such as the amount of H2O2, catalyst dose, pH, and initial concentration of dye were investigated during the experiments. Finally, the decolorization process with the falling-film photocatalytic reactor was optimized using response surface methodology (RSM). The physical characteristics showed that the average particle size of the synthesized ZnO-Nd was 40 nm. Doping ZnO with Nd reduced the photocatalyst energy bandgap by 14%. The results indicated that the optimum amount of catalyst dose and pH level was 0.1 g/L and 5, respectively. The simultaneous usage of H2O2 and ZnO-Nd with an H2O2/dye ratio of two increased dye removal performance by 90%. The results demonstrated that the developed equations can be applied to predict the performance of the falling-film photoreactor. This study showed that using the nano ZnO-Nd photocatalyst in a falling-film photocatalytic reactor under optimum operating conditions is an appropriate way to remove RR198 from water.
Lethal and Sublethal Responses of Hydropsyche pellucidula (Insecta, Trichoptera) to Commercial Polypropylene Microplastics after Different Preconditioning Treatments
Authors: Piccardo Manuela , Bertoli Marco , Pastorino Paolo , Barceló Damià , Provenza Francesca , Lesa Davide , Anselmi Serena , Elia Antonia , Prearo Marino , Pizzul Elisabetta , Renzi Monia
Published: October 2021

Microplastics (MPs) pose biological and chemical hazards in aquatic and terrestrial food webs across the globe. Research on microplastic contamination has long focused on marine ecosystems, whereas the toxicological impact on freshwater organisms is still little explored. In this study, the lethal and sublethal response of the freshwater macroinvertebrate Hydropsyche pellucidula exposed to polypropylene MPs after different pre-conditioning treatments was assessed. Field samples were collected in a riverine system (Vipacco river; northeast Italy) to assess the characteristics of the MPs in the aquatic environment Both water and sediment were contaminated by MPs (3.73 ± 2.11 items m−3 per min and 3.33 ± 4.16 items dm−3, respectively). The chemical MPs composition included polystyrene, polyethylene terephthalate, polyurethane, polyamide, polypropylene, and polyethylene. Polypropylene (PP), although not the most abundant polymer recorded in the study area, was preferred over the other types according to its abundance in freshwater and H. pellucidula feeding behavior. A housing test was performed to recreate the natural conditions of larvae sampled for a reliable response to the ecotoxicological tests. The microplastics underwent either preconditioning with Vipacco River water (PP-river) and surfactant Triton X-100 (PP-sf) or no pre-treatment (PP). Submersion of microplastics in 10 µg L−1 of surfactant solution for 24 h was sufficient to induce consistent spectral changes and modify the chemical profile of the plastic surface. Mortality rate differed according to treatment: PP and PP-river > positive control > PP-sf > negative control. Integrated biomarker response (IBRv2) and analysis of oxidative stress biomarker levels showed a greater response of superoxide dismutase and lipid peroxidation (malondialdehyde) in larvae treated with PP conditioned in surfactant. Our findings enhance knowledge on the toxicity of PP and conditioning phases on H. pellucidula larvae.
Stem-Cell Transplantation in Adult Patients with Relapsed/Refractory Hodgkin Lymphoma
Authors: Stavrik Sonja Genadieva , Sureda Anna
Published: October 2021

Although the majority of patients with Hodgkin lymphoma (HL) are cured with initial therapy, in 85–90% of early stage and 70–80% of advanced-stage disease cases, relapse remains a major problem. Autologous stem-cell transplantation (auto-HCT) after salvage chemotherapy is currently considered to be the standard of care for patients who relapse after first-line chemotherapy or for whom first-line treatment fails. The curative capacity of auto-HCT has been improving with the introduction of new drug-based salvage strategies and consolidation strategies after auto-HCT. Allogeneic stem-cell transplantation (allo-HCT) represents a reasonable treatment option for young patients who relapse or progress after auto-HCT and have chemosensitive disease at the time of transplantation. Allo-HCT is a valid treatment strategy for patients with relapse/refractory HL (r/r HL) because the results have improved over time, mainly with the safe combination of allo-HCT and new drugs. Bearing in mind that outcomes after haploidentical stem-cell transplantation (haplo-HCT) are comparable with those for matched sibling donors and matched unrelated donors, haplo-HCT is now the preferred alternative donor source for patients with r/r HL without a donor or when there is urgency to find a donor if a matched related donor is not present. The development of new drugs such as anti-CD 30 monoclonal antibodies and checkpoint inhibitors (CPI) for relapsed or refractory HL has demonstrated high response rates and durable remissions, and challenged the role and timing of HCT. The treatment of patients with HL who develop disease recurrence or progression after allo-HCT remains a real challenge and an unmet need.
Antimicrobial Resistance Situational Analysis 2019–2020: Design and Performance for Human Health Surveillance in Uganda
Authors: Mugerwa Ibrahimm , Nabadda Susan N. , Midega Janet , Guma Consolata , Kalyesubula Simeon , Muwonge Adrian
Published: October 2021

Antibiotic resistance and its mechanisms have been known for over six decades, but global efforts to characterize its routine drivers have only gained momentum in the recent past. Drivers of clinical and community resistance go beyond just clinical practice, which is why one-health approaches offer the most realistic option for controlling antibiotic resistance. It is noteworthy that the emergence of resistance occurs naturally in the environment, but akin to climate change, the current accelerated emergence and spread bears hallmarks of anthropomorphic influence. If left unchecked, this can undo the medical and agricultural advancements of the last century. The WHO recommends that nations develop, adopt, and implement strategies that track the changing trends in antibiotic resistance levels to tackle this problem. This article examines efforts and progress in developing and implementing a human health antimicrobial resistance surveillance strategy in Uganda. We do so within the context of the National Action Plan for tackling antimicrobial resistance (AMR-NAP) launched in 2018. We discuss the technical milestones and progress in implementing surveillance of GLASS priority pathogens under this framework. The preliminary output of the framework examines the performance and compares AMR and AMU surveillance data to explain observed trends. We conclude that Uganda is making progress in developing and implementing a functional AMR surveillance strategy for human health.
Current Trends and Limitations in Dengue Antiviral Research
Authors: Obi Juliet , Gutiérrez-Barbosa Hernando , Chua Joel , Deredge Daniel
Published: October 2021

Dengue is the most prevalent arthropod-borne viral disease worldwide and affects approximately 2.5 billion people living in over 100 countries. Increasing geographic expansion of Aedes aegypti mosquitoes (which transmit the virus) has made dengue a global health concern. There are currently no approved antivirals available to treat dengue, and the only approved vaccine used in some countries is limited to seropositive patients. Treatment of dengue, therefore, remains largely supportive to date; hence, research efforts are being intensified for the development of antivirals. The nonstructural proteins, 3 and 5 (NS3 and NS5), have been the major targets for dengue antiviral development due to their indispensable enzymatic and biological functions in the viral replication process. NS5 is the largest and most conserved nonstructural protein encoded by flaviviruses. Its multifunctionality makes it an attractive target for antiviral development, but research efforts have, this far, not resulted in the successful development of an antiviral targeting NS5. Increase in structural insights into the dengue NS5 protein will accelerate drug discovery efforts focused on NS5 as an antiviral target. In this review, we will give an overview of the current state of therapeutic development, with a focus on NS5 as a therapeutic target against dengue.
Black Hole Spin and Stellar Flyby Periastron Shift
Authors: Tito Elizabeth P. , Pavlov Vadim I.
Published: October 2021

For a scenario of a close flyby of a compact star near a spinning black hole, we provide analytical and numerical estimates for the shift of trajectory periastron due to relativistic (beyond post-Newtonian) effects. More specifically, we derived a generalized expression (not limited to quasi-circular or elliptical orbits) directly linking the periastron shift and the spin of the black hole. The expression permits the estimation of black hole spin based on astronomical tracking of locations of stars traveling along highly eccentric (parabolic and hyperbolic) trajectories in close vicinity of a black hole. We also demonstrate how stars traveling on hyperbolic or parabolic trajectories may be (temporarily) captured onto quasi-circular orbits around black holes, and we quantitatively examine conditions for such scenarios.
Fuzzy Instantons in Landscape and Swampland: Review of the Hartle–Hawking Wave Function and Several Applications
Authors: Yeom Dong-han
Published: October 2021

The Euclidean path integral is well approximated by instantons. If instantons are dynamical, they will necessarily be complexified. Fuzzy instantons can have multiple physical applications. In slow-roll inflation models, fuzzy instantons can explain the probability distribution of the initial conditions of the universe. Although the potential shape does not satisfy the slow-roll conditions due to the swampland criteria, the fuzzy instantons can still explain the origin of the universe. If we extend the Euclidean path integral beyond the Hartle–Hawking no-boundary proposal, it becomes possible to examine fuzzy Euclidean wormholes that have multiple physical applications in cosmology and black hole physics.
High-Order Multipole and Binary Love Number Universal Relations
Authors: Godzieba Daniel A. , Radice David
Published: October 2021

Using a data set of approximately 2 million phenomenological equations of state consistent with observational constraints, we construct new equation-of-state-insensitive universal relations that exist between the multipolar tidal deformability parameters of neutron stars, Λl, for several high-order multipoles (l=5,6,7,8), and we consider finite-size effects of these high-order multipoles in waveform modeling. We also confirm the existence of a universal relation between the radius of the 1.4M⊙ NS, R1.4 and the reduced tidal parameter of the binary, Λ˜, and the chirp mass. We extend this relation to a large number of chirp masses and to the radii of isolated NSs of different mass M, RM. We find that there is an optimal value of M for every M such that the uncertainty in the estimate of RM is minimized when using the relation. We discuss the utility and implications of these relations for the upcoming LIGO O4 run and third-generation detectors.
Hybrid Stars with Color Superconducting Cores in an Extended FCM Model
Authors: Curin Daniela , Ranea-Sandoval Ignacio Francisco , Mariani Mauro , Orsaria Milva Gabriela , Weber Fridolin
Published: October 2021

We investigate the influence of repulsive vector interactions and color superconductivity on the structure of neutron stars using an extended version of the field correlator method (FCM) for the description of quark matter. The hybrid equation of state is constructed using the Maxwell description, which assumes a sharp hadron-quark phase transition. The equation of state of hadronic matter is computed for a density-dependent relativistic lagrangian treated in the mean-field approximation, with parameters given by the SW4L nuclear model. This model described the interactions among baryons in terms of σ, ω, ρ, σ*, and ϕ mesons. Quark matter is assumed to be in either the CFL or the 2SC+s color superconducting phase. The possibility of sequential (hadron-quark, quark-quark) transitions in ultra-dense matter is investigated. Observed data related to massive pulsars, gravitational-wave events, and NICER are used to constrain the parameters of the extended FCM model. The successful equations of state are used to explore the mass-radius relationship, radii, and tidal deformabilities of hybrid stars. A special focus lies on investigating consequences that slow or fast conversions of quark-hadron matter have on the stability and the mass-radius relationship of hybrid stars. We find that if slow conversion should occur, a new branch of stable massive stars would exist whose members have radii that are up to 1.5 km smaller than those of conventional neutron stars of the same mass. Such objects could be possible candidates for the stellar high-mass object of the GW190425 binary system.
A New Sample of Gamma-Ray Emitting Jetted Active Galactic Nuclei—Preliminary Results
Authors: Foschini Luigi , Lister Matthew L. , Antón Sonia , Berton Marco , Ciroi Stefano , Marchã Maria J. M. , Tornikoski Merja , Järvelä Emilia , Romano Patrizia , Vercellone Stefano , Dalla Bontà Elena
Published: October 2021

We are compiling a new list of gamma-ray jetted active galactic nuclei (AGN), starting from the fourth catalog of point sources of the Fermi Large Area Telescope (LAT). Our aim is to prepare a list of jetted AGN with known redshifts and classifications to be used to calibrate jet power. We searched in the available literature for all the published optical spectra and multiwavelength studies useful to characterize the sources. We found new, missed, or even forgotten information leading to a substantial change in the redshift values and classification of many sources. We present here the preliminary results of this analysis and some statistics based on the gamma-ray sources with right ascension within the interval 0h--12h (J2000). Although flat-spectrum radio quasars and BL Lac objects are still the dominant populations, there is a significant increase in the number of other objects, such as misaligned AGN, narrow-line Seyfert 1 galaxies, and Seyfert galaxies. We also introduced two new classes of objects: changing-look AGN and ambiguous sources. About one third of the sources remain unclassified.
Correlations between Spatial Attributes and Visitor Stay in Chinese Gardens: A Case Study of the Ningbo Tianyige Museum Gardens
Authors: Yu Jia , Yokota Takashi , Itami Emiko , Yang Jing
Published: October 2021

Despite the growing popularity of Chinese gardens, few studies have explored Chinese garden tourism and the relationship between garden space and visitor behavior. Addressing this gap, this study examines the correlations between spatial attributes and visitor stay distribution in the Ningbo Tianyige Museum gardens. This study divided the garden space into twenty units across four types—water, architecture, veranda, and rockery—and identified spatial attributes using measurements and configurational calculations. Visitor stay data was comprised of 1061 cases with a stay interval of more than 30 s in three investigation periods. Results produced three primary findings. First, architecture and water spaces had the highest visitor stay density, followed by veranda space and then rockery space. Second, there is a correlation between visitor stay density and six spatial attributes: integration, choice, width, length, enclosure ratio, and seating. Third, although each type has distinctive attributes, they can be divided into two groups: (1) spacious and highly accessible open spaces (water and architecture types); (2) long narrow spaces with low accessibility and abundant seating facilities (veranda and rockery types). By exploring the relationship between Chinese gardens and modern tourism, this study provides valuable insights and suggestions for the planning and management of Chinese garden tourism.
Preparing for COVID-2x: Urban Planning Needs to Regard Urological Wastewater as an Invaluable Communal Public Health Asset and Not as a Burden
Authors: Spennemann Dirk H. R.
Published: October 2021

Prior to the COVID-19 pandemic, the analysis of urological wastewater had been a matter of academic curiosity and community-wide big-picture studies looking at drug use or the presence of select viruses such as Hepatitis. The COVID-19 pandemic saw systematic testing of urological wastewater emerge as a significant early detection tool for the presence of SARS-CoV-2 in a community. Even though the pandemic still rages in all continents, it is time to consider the post-pandemic world. This paper posits that urban planners should treat urological wastewater as a communal public health asset and that future sewer design should allow for stratified multi-order sampling.
Influenza Vaccination Coverage among Older Adults with Hypertension in Shenzhen, China: A Cross-Sectional Survey during the COVID-19 Pandemic
Authors: Li Qiushuang , Zhang Minyi , Chen Hongbiao , Wu Fei , Xian Juxian , Zheng Liting , Liang Minyi , Cao He , Zhou Xiaofeng , Gu Zihao , Lin Qihui , Chen Qing
Published: October 2021

Background: Older individuals with hypertension are at a high risk of being infected with influenza. However, there have been few studies investigating the influenza vaccination status among older people with hypertension. The present work aimed to estimate the vaccination coverage and determine the predictors of seasonal influenza vaccinations among hypertensive patients aged over 60 years in Shenzhen, China. Method: The study used data from an online cross-sectional survey that was conducted in Shenzhen City, China, in October 2020. Frequencies and proportions of all the variables including sociodemographic characteristics and health-related information were described and tabulated based on the influenza vaccination status. Bivariate and multivariable logistic regression analyses were used to identify independent predictors associated with the influenza vaccination. Results: A total of 5216 older people with hypertension aged above 60 years were recruited. Overall, only 4.7% had received an influenza vaccine in the latest influenza season. Using the action toward being vaccinated as the primary outcome, the multivariable regression analysis showed that participants aged over 80 years (aOR 2.957, 95% CI: 1.784–4.900), obtaining higher education levels (aOR 1.424, 95% CI: 1.060–1.914 for high school, aOR 1.681, 95% CI: 1.066–2.650 for college or above), living with a partner (aOR 1.432, 95% CI: 1.068–1.920), using a family doctor (aOR 2.275, 95% CI: 1.744–2.968), and taking a physical examination 1–2 and ≥3 times each year (aOR 2.107, 95% CI: 1.601–2.772 and aOR 2.118, 95% CI: 1.083–4.143, respectively) were more likely to be vaccinated. In contrast, smokers had less likelihood of having the influenza vaccination than non-smokers (aOR 1.829, 95% CI: 1.208–2.767). Conclusions: The coverage rate of influenza vaccinations is far away from optimistic among older adults with hypertension. Additional works should be undertaken immediately to improve the influenza vaccination status.
COVID-19 Vaccine Hesitancy among Parents of Children and Adolescents Living in Brazil
Authors: Bagateli Leonardo Evangelista , Saeki Edna Yayoi , Fadda Marta , Agostoni Carlo , Marchisio Paola , Milani Gregorio Paolo
Published: October 2021

Background: The immunization of large portions of populations in low/middle-income countries is considered one of the key measures to limit the development of new SARS-CoV-2 variants. However, parental vaccine hesitancy might be an important obstacle to pediatric vaccination. The aim of this survey was to study the prevalence and extent of COVID-19 vaccine hesitancy among parents of children and adolescents living in Brazil. Methods: Caregivers of children and adolescents referred to the emergency department of Hospital Estadual de Bauru, São Paulo (Brazil) were invited to fill in a validated questionnaire on vaccine hesitancy and to report their willingness for themselves and their offspring to receive a COVID-19 vaccine. Results: A total of 501 consecutive caregivers filled in the survey. Response rate was 100%. A minority (N = 14, 2.8%) of caregivers were hesitant about vaccines. Despite this, half of them declared they were willing to vaccinate their offspring against COVID-19. Conclusions: This survey identifies that vaccine hesitancy is very low among caregivers living in Brazil and that even many of the hesitant caregivers are willing to vaccinate their offspring against COVID-19. This study highlights the importance of offering the COVID-19 vaccination to the whole population, including subjects that present uncertainty about other vaccines.
Identification of HLA-A2-Restricted Mutant Epitopes from Neoantigens of Esophageal Squamous Cell Carcinoma
Authors: Wang Zhiwei , Ran Ling , Chen Chunxia , Shi Ranran , Dong Yu , Li Yubing , Zhou Xiuman , Qi Yuanming , Zhu Pingping , Gao Yanfeng , Wu Yahong
Published: October 2021

Esophageal squamous cell carcinoma (ESCC), one of the deadliest gastrointestinal cancers, has had limited effective therapeutic strategies up to now. Accumulating evidence suggests that effective immunotherapy in cancer patients has been associated with T cells responsive to mutant peptides derived from neoantigens. Here, we selected 35 human leukocyte antigen-A2 (HLA-A2)-restricted mutant (MUT) peptides stemmed from neoantigens of ESCC. Among them, seven mutant peptides had potent binding affinity to HLA-A*0201 molecules and could form a stable peptide/HLA-A*0201 complex. Three mutant peptides (TP53-R267P, NFE2L2-D13N, and PCLO-E4090Q) of those were immunogenic and could induce the cytotoxic T lymphocytes (CTLs) recognizing mutant peptides presented on transfected cells in an HLA-A2-restricted and MUT peptide-specific manner. In addition, the CTL response in immunized HLA-A2.1/Kb transgenic (Tg) mice was enhanced by coupling MUT peptides to peptide WH, a peptide delivery carrier targeting Clec9a+ DCs. Then, the possible binding model conversions between the WT and MUT candidate peptides were analyzed by docking with the pockets of HLA-A*0201 molecule. We therefore propose a novel strategy and epitopes for immunotherapy of ESCC based on neoantigens.
Thymus Gland: A Double Edge Sword for Coronaviruses
Authors: Al-Suhaimi Ebtesam A. , Aljafary Meneerah A. , Alkhulaifi Fadwa M. , Aldossary Hanan A. , Alshammari Thamer , AL-Qaaneh Ayman , Aldahhan Razan , Alkhalifah Zahra , Gaymalov Zagit Z. , Shehzad Adeeb , Homeida Abdelgadir M.
Published: October 2021

The thymus is the main lymphoid organ that regulates the immune and endocrine systems by controlling thymic cell proliferation and differentiation. The gland is a primary lymphoid organ responsible for generating mature T cells into CD4+ or CD8+ single-positive (SP) T cells, contributing to cellular immunity. Regarding humoral immunity, the thymic plasma cells almost exclusively secrete IgG1 and IgG3, the two main complement-fixing effector IgG subclasses. Deformity in the thymus can lead to inflammatory diseases. Hassall’s corpuscles’ epithelial lining produces thymic stromal lymphopoietin, which induces differentiation of CDs thymocytes into regulatory T cells within the thymus medulla. Thymic B lymphocytes produce immunoglobulins and immunoregulating hormones, including thymosin. Modulation in T cell and naive T cells decrement due to thymus deformity induce alteration in the secretion of various inflammatory factors, resulting in multiple diseases. Influenza virus activates thymic CD4+ CD8+ thymocytes and a large amount of IFNγ. IFNs limit virus spread, enhance macrophages’ phagocytosis, and promote the natural killer cell restriction activity against infected cells. Th2 lymphocytes-produced cytokine IL-4 can bind to antiviral INFγ, decreasing the cell susceptibility and downregulating viral receptors. COVID-19 epitopes (S, M, and N proteins) with ≥90% identity to the SARS-CoV sequence have been predicted. These epitopes trigger immunity for antibodies production. Boosting the immune system by improving thymus function can be a therapeutic strategy for preventing virus-related diseases. This review aims to summarize the endocrine-immunoregulatory functions of the thymus and the underlying mechanisms in the prevention of COVID-19.
The Use of Digital Technologies to Support Vaccination Programmes in Europe: State of the Art and Best Practices from Experts’ Interviews
Authors: Odone Anna , Gianfredi Vincenza , Sorbello Sebastiano , Capraro Michele , Frascella Beatrice , Vigezzi Giacomo Pietro , Signorelli Carlo
Published: October 2021

Digitalisation offers great potential to improve vaccine uptake, supporting the need for effective life-course immunisation services. We conducted semi-structured in-depth interviews with public health experts from 10 Western European countries (Germany, Greece, Italy, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, and the United Kingdom) to assess the current level of digitalisation in immunisation programmes and retrieve data on interventions and best practices. Interviews were performed using an ad hoc questionnaire, piloted on a sample of national experts. We report a mixed level of digital technologies deployment within vaccination services across Europe: Some countries are currently developing eHealth strategies, while others have already put in place robust programmes. Institutional websites, educational videos, and electronic immunisation records are the most frequently adopted digital tools. Webinars and dashboards represent valuable resources to train and support healthcare professionals in immunisation services organisation. Text messages, email-based communication, and smartphone apps use is scattered across Europe. The main reported barrier to the implementation of digital-based programmes is the lack of resources and shared standards. Our study offers a comprehensive picture of the European context and shows the need for robust collaboration between states and international institutions to share best practices and inform the planning of digital intervention models with the aim of countering vaccine hesitancy and increasing vaccine uptake.
Nanotechnology Interventions in the Management of COVID-19: Prevention, Diagnosis and Virus-Like Particle Vaccines
Authors: Balkrishna Acharya , Arya Vedpriya , Rohela Akansha , Kumar Ashwani , Verma Rachna , Kumar Dinesh , Nepovimova Eugenie , Kuca Kamil , Thakur Naveen , Thakur Nikesh , Kumar Pankaj
Published: October 2021

SARS-CoV-2 claimed numerous lives and put nations on high alert. The lack of antiviral medications and the small number of approved vaccines, as well as the recurrence of adverse effects, necessitates the development of novel treatment ways to combat COVID-19. In this context, using databases such as PubMed, Google Scholar, and Science Direct, we gathered information about nanotechnology’s involvement in the prevention, diagnosis and virus-like particle vaccine development. This review revealed that various nanomaterials like gold, polymeric, graphene and poly amino ester with carboxyl group coated magnetic nanoparticles have been explored for the fast detection of SARS-CoV-2. Personal protective equipment fabricated with nanoparticles, such as gloves, masks, clothes, surfactants, and Ag, TiO2 based disinfectants played an essential role in halting COVID-19 transmission. Nanoparticles are used not only in vaccine delivery, such as lipid nanoparticles mediated transport of mRNA-based Pfizer and Moderna vaccines, but also in the development of vaccine as the virus-like particles elicit an immune response. There are now 18 virus-like particle vaccines in pre-clinical development, with one of them, developed by Novavax, reported being in phase 3 trials. Due to the probability of upcoming COVID-19 waves, and the rise of new diseases, the future relevance of virus-like particles is imperative. Furthermore, psychosocial variables linked to vaccine reluctance constitute a critical problem that must be addressed immediately to avert pandemic.
Longitudinal Humoral Responses after COVID-19 Vaccination in Peritoneal and Hemodialysis Patients over Twelve Weeks
Authors: Speer Claudius , Schaier Matthias , Nusshag Christian , Töllner Maximilian , Buylaert Mirabel , Kälble Florian , Reichel Paula , Grenz Julia , Süsal Caner , Zeier Martin , Schnitzler Paul , Morath Christian , Klein Katrin , Benning Louise
Published: October 2021

It has been demonstrated that patients on hemo- or peritoneal dialysis are particularly susceptible to SARS-CoV-2 infection and impaired seroconversion compared to healthy controls. Follow-up data on vaccination response in dialysis patients is limited but is greatly needed to individualize and guide (booster) vaccination strategies. In this prospective, multicenter study we measured anti-spike S1 and neutralizing antibodies in 124 hemodialysis patients, 41 peritoneal dialysis patients, and 20 age- and sex-matched healthy controls over 12 weeks after homologous BNT162b2 vaccination. Compared to healthy controls, both hemodialysis and peritoneal dialysis patients had lower anti-S1 IgG antibodies (median (IQR) 7.0 (2.8–24.3) and 21.8 (5.8–103.9) versus 134.9 (23.8–283.6), respectively; p < 0.001 and p < 0.05) and a reduced SARS-CoV-2 spike protein–ACE2 binding inhibition caused by vaccine-induced antibodies (median (IQR) 56% (40–81) and 77% (52–89) versus 96% (90–98), respectively; p < 0.001 and p < 0.01) three weeks after the second vaccination. Twelve weeks after the second vaccination, the spike protein–ACE2 binding inhibition significantly decreased to a median (IQR) of 45% (31–60) in hemodialysis patients and 55% (36–78) in peritoneal dialysis patients, respectively (p < 0.001 and p < 0.05). Peritoneal dialysis patients mounted higher antibody levels compared with hemodialysis patients at all time points during the 12-week follow-up. Individual booster vaccinations in high-risk individuals without seroconversion or rapidly waning neutralizing antibody levels are required and further data on the neutralization of emerging variants of concern in these patients are urgently needed.
Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector
Authors: van Diepen Michiel , Chapman Rosamund , Douglass Nicola , Whittle Leah , Chineka Nicole , Galant Shireen , Cotchobos Christian , Suzuki Akiko , Williamson Anna-Lise
Published: October 2021

Attenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vaccine vectors, to target both LSDV, as well as other pathogens, including human infectious agents. Historically, these vaccine strains and recombinants were generated in primary (lamb) testis (LT) cells, Madin–Darby bovine kidney (MDBK) cells or in eggs. Growth in eggs is a laborious process, the use of primary cells has the potential to introduce pathogens and MDBK cells are known to harbor bovine viral diarrhea virus (BVDV). In this study, data is presented to show the growth of an attenuated LSDV strain in baby hamster kidney (BHK-21) cells. Subsequently, a recombinant LSDV vaccine was generated in BHK-21 cells. Partial growth was also observed in rabbit kidney cells (RK13), but only when the vaccinia virus host range gene K1L was expressed. Despite the limited growth, the expression of K1L was enough to serve as a positive selection marker for the generation of recombinant LSDV vaccines in RK13 cells. The simplification of generating (recombinant) LSDV vaccines shown here should increase the interest for this platform for future livestock vaccine development and, with BHK-21 cells approved for current good manufacturing practice, this can be expanded to human vaccines as well.
Attitudes and Intentions toward COVID-19 Vaccination among Spanish Adults: A Descriptive Cross-Sectional Study
Authors: Mosteiro-Miguéns Diego Gabriel , Roca Daniel De Bernardo , Domínguez-Martís Eva María , Vieito-Pérez Natalia , Álvarez-Padín Pilar , Novío Silvia
Published: October 2021

Vaccination against SARS-CoV-2 is postulated as the most effective measure to control the COVID-19 pandemic. However, the use of other protection measures is necessary to efficiently combat the spread of the virus. The aim of the present study was to determine the attitudes and intentions toward COVID-19 vaccination among non-regular social media users in Spain and to analyze how these factors could condition the acceptance of other personal protective measures once an individual has received the COVID-19 vaccine. A cross-sectional design was used in this work. In total, 719 subjects, ≥18 years old and of both sexes, were recruited from primary public healthcare centers to self-complete a questionnaire between March and April 2021. The majority of participants had a positive attitude toward vaccination and showed high levels of intention to be vaccinated. Likewise, except those participants who considered the vaccine to be the most effective measure to fight the COVID-19 pandemic, the rest of the participants highlighted the importance of continuing to limit social interactions and/or wearing masks even after being vaccinated. Since vaccination can create a perception of total immunity against SARS-CoV-2, it is necessary that healthcare staff organize effective awareness campaigns on the importance of maintaining personal protective measures until vaccination coverage is greater.
COVID-19 Vaccine Concerns about Safety, Effectiveness, and Policies in the United States, Canada, Sweden, and Italy among Unvaccinated Individuals
Authors: Piltch-Loeb Rachael , Harriman Nigel Walsh , Healey Julia , Bonetti Marco , Toffolutti Veronica , Testa Marcia A. , Su Max , Savoia Elena
Published: October 2021

Despite the effectiveness of the COVID-19 vaccine, global vaccination distribution efforts have thus far had varying levels of success. Vaccine hesitancy remains a threat to vaccine uptake. This study has four objectives: (1) describe and compare vaccine hesitancy proportions by country; (2) categorize vaccine-related concerns; (3) rank vaccine-related concerns; and (4) compare vaccine-related concerns by country and hesitancy status in four countries—the United States, Canada, Sweden, and Italy. Using the Pollfish survey platform, we sampled 1000 respondents in Canada, Sweden, and Italy and 750 respondents in the United States between 21–28 May 2021. Results showed vaccine-related concerns varied across three topical areas—vaccine safety and government control, vaccine effectiveness and population control, and freedom. For each thematic area, the top concern was statistically significantly different in each country and among the hesitant and non-hesitant subsamples within each county. Concerns related to freedom were the most universal. Understanding the specific concerns among individuals when it comes to the COVID-19 vaccine can help to inform public communications and identify which, if any, salient narratives are global.
Immunological Response to COVID-19 Vaccination in Ovarian Cancer Patients Receiving PARP Inhibitors
Authors: Liontos Michalis , Terpos Evangelos , Markellos Christos , Zagouri Flora , Briasoulis Alexandros , Katsiana Ioanna , Skafida Efthymia , Fiste Oraianthi , Kunadis Elena , Andrikopoulou Angeliki , Kaparelou Maria , Koutsoukos Konstantinos , Gavriatopoulou Maria , Kastritis Efstathios , Trougakos Ioannis P. , Dimopoulos Meletios-Athanasios
Published: October 2021

Objective: Vaccination for SARS-CoV-2 provides significant protection against the infection in the general population. However, limited data exist for cancer patients under systemic therapy. Methods: In this cohort, we prospectively enrolled cancer patients treated with PARPi as well as healthy volunteers in order to study the kinetics of anti-SARS-CoV-2 antibodies (NAbs) after COVID-19 vaccination. Baseline demographics, co-morbidities, and NAb levels were compared between the two groups. Results: The results of the cohort of 36 patients receiving PARP inhibitors are presented here. Despite no new safety issues being noticed, their levels of SARS-CoV-2 neutralizing antibodies were significantly lower in comparison to matched healthy volunteers up to day 30 after the second dose. Conclusions: These results suggest that maintaining precautions against COVID-19 is essential for cancer patients and should be taken into consideration for the patients under treatment, while further exploration is needed to reduce the uncertainty of SARS-CoV-2 immunity among cancer patients under treatment.
Association between Immunoglobulin G Levels and Adverse Effects Following Vaccination with the BNT162b2 Vaccine among Japanese Healthcare Workers
Authors: Otani Jun , Ohta Ryuichi , Sano Chiaki
Published: October 2021

The purpose of the study was to assess the association between the amount of immunoglobulin G (IgG) and the duration of adverse effects of COVID-19 vaccinations in the Japanese population. This cross-sectional study was conducted from April 2020 to August 2021 among workers at a community hospital. All participants received two doses of the BNT162b2 vaccine (Pfizer-BioNTech) in March and April 2021. Vaccine side effects were measured using a self-administered questionnaire. Serum anti-SARS-CoV-2 IgG was measured 3 months after vaccination. There was a total of 338 participants (mean age: 44.7 years). The incidence of adverse reactions after vaccination was higher in women. Adverse reactions associated with higher IgG levels included: erythema at the injection site after the first dose; induration and inflammation at the injection site; and systemic symptoms, e.g., fever and headache after the second dose. IgG levels were higher in younger participants. These findings could mitigate fears regarding the mild adverse effects of the COVID-19 vaccine and encourage uptake of the BNT162b2 vaccine.
Correlates of COVID-19 Vaccine Acceptance, Hesitancy and Refusal among Employees of a Safety Net California County Health System with an Early and Aggressive Vaccination Program: Results from a Cross-Sectional Survey
Authors: Gatto Nicole M. , Lee Jerusha E. , Massai Donatella , Zamarripa Susanna , Sasaninia Bijan , Khurana Dhruv , Michaels Kelsey , Freund Deborah , Nightingale Judi , Firek Anthony
Published: October 2021

Since health professionals provide frontline care to COVID-19 patients, information on vaccine acceptance among healthcare workers is needed. We developed and implemented an anonymous internet-based cross-sectional survey with direct solicitation among employees of a safety net health system. Items queried demographic and health-related characteristics, experience with and knowledge of COVID-19, and determinants of decisions to vaccinate. COVID-19 vaccine acceptance groups (acceptors, hesitant, refusers) were defined; an adapted version of the WHO vaccine hesitancy scale was included. The survey demonstrated good reliability (Cronbach’s alpha = 0.92 for vaccine hesitancy scale; 0.93 for determinants). General linear and logistic regression methods examined factors which were univariately associated with vaccine hesitancy and vaccine acceptance, respectively. Multivariable models were constructed with stepwise model-building procedures. Race/ethnicity, marital status, job classification, immunocompromised status, flu vaccination and childhood vaccination opinions independently predicted hesitancy scale scores. Gender, education, job classification and BMI independently predicted acceptance, hesitancy, and refusal groups. Among hesitant employees, uncertainty was reflected in reports of motivating factors influencing their indecision. Despite a strong employee-support environment and job protection, respondents reported physical and mental health effects. The appreciation of varied reasons for refusing vaccination should lead to culturally sensitive interventions to increase vaccination rates amongst healthcare workers.
A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles
Authors: González-Domínguez Irene , Lorenzo Elianet , Bernier Alice , Cervera Laura , Gòdia Francesc , Kamen Amine
Published: October 2021

Gag-based virus-like particles (VLPs) have high potential as scaffolds for the development of chimeric vaccines and delivery strategies. The production of purified preparations that can be preserved independently from cold chains is highly desirable to facilitate distribution and access worldwide. In this work, a nimble purification has been developed, facilitating the production of Gag VLPs. Suspension-adapted HEK 293 cells cultured in chemically defined cell culture media were used to produce the VLPs. A four-step downstream process (DSP) consisting of membrane filtration, ion-exchange chromatography, polishing, and lyophilization was developed. The purification of VLPs from other contaminants such as host cell proteins (HCP), double-stranded DNA, or extracellular vesicles (EVs) was confirmed after their DSP. A concentration of 2.2 ± 0.8 × 109 VLPs/mL in the lyophilized samples was obtained after its storage at room temperature for two months. Morphology and structural integrity of purified VLPs was assessed by cryo-TEM and NTA. Likewise, the purification methodologies proposed here could be easily scaled up and applied to purify similar enveloped viruses and vesicles.
Canine-Origin Platelet-Rich Fibrin as an Effective Biomaterial for Wound Healing in Domestic Cats: A Preliminary Study
Authors: Soares Carla S. , Dias Isabel R. , Pires Maria A. , Carvalho Pedro P.
Published: October 2021

Platelet-rich fibrin (PRF) is a recent platelet-based biomaterial, poised as an innovative regenerative strategy for the treatment of wounds from different etiologies. PRF is defined as a biodegradable scaffold containing elevated amounts of platelets and leukocytes having the capability to release high concentrations of bioactive structural proteins and acting as a temporal release healing hemoderivative. This study aimed to evaluate the performance of canine-origin PRF, obtained from blood of screened donors, as a regenerative biomaterial suitable for the treatment of critical wounds in felines. Four short-hair felines with naturally occurring wounds were enrolled in this study. Three of the wounds were considered infected. Each PRF treatment was the result of the grafting of newly produced PRFs at the recipient area. The PRF treatment was initially performed two to three times per week, followed by single weekly treatments. The study was finalized when complete wound closure was achieved. No topical antimicrobial/antiseptic treatment was applied. The present research demonstrated that xenogenic PRFs significantly induced healthy vascularized granulation tissue in lesions with soft tissue deficit, also prompting the epithelization at the injured site. No rejection, necrosis, or infection signs were recorded. Additionally, PRF-therapy was revealed to be a biological cost-effective treatment, accelerating the wound healing process.
Autologous Bone Marrow Mononuclear Cells (BMMCs) for the Treatment of Uncomplicated Grade 2 Ununited Anconeal Process (UAP) in Six Dogs: Preliminary Results
Authors: Crovace Alberto Maria , Lacitignola Luca , Di Comite Mariasevera , Esposito Cosimo , Guarracino Alessandro , Francioso Edda , Staffieri Francesco , Crovace Antonio
Published: October 2021

The aim of this study was to report the results of autologous bone marrow mononuclear cell (BMMC) transplantation as a minimally invasive treatment for grade 2 UAP in dogs. This was an observational case series on six German shepherd dogs affected by grade 2 UAP as defined according to their clinical condition as well as radiographic and CT findings. Bone marrow was collected from the iliac crest and the mononuclear fraction was separated with density gradient centrifugation. Cells were suspended in fibrin glue before BMMC administration and implanted via transcutaneous injection under IB or CT guidance, using a spinal needle directly inserted into the ossification centre between the anconeal process and the olecranon. Clinical and radiographic follow-up was performed for up to 6 months. Microradiographic assessment was performed on one dog that died of other causes. A progressive reduction of pain within 3 weeks after BMMC administration was observed in all dogs, with gradually increased weight bearing on the affected limb. Radiographic and CT follow-up revealed the progressive fusion of the ossification centre at 90 days without any signs of secondary OA. The examination of microradiographs showed newly formed bone tissue in which a residue of calcified cartilage was present at the site of BMMC implantation. On the basis of these results, BMMC therapy for grade 2 UAP may be considered to be an effective and minimally invasive treatment option for dogs.
Feline Leishmaniosis in Northwestern Italy: Current Status and Zoonotic Implications
Authors: Elmahallawy Ehab Kotb , Zanet Stefania , Poggi Marco , Alsharif Khalaf F. , Agil Ahmad , Trisciuoglio Anna , Ferroglio Ezio
Published: October 2021

Leishmaniasis remains one of the major neglected tropical diseases. The epidemiological profile of the disease comprises a wide range of hosts, including dogs and cats. Despite several studies about feline Leishmaniosis, the role of cats in disease epidemiology and its clinical impact is still debated. The present study raises awareness about the impact of leishmaniasis in cats from an endemic region in of Northwestern Italy (Liguria). A total number of 250 serum and 282 blood samples were collected from cats, then assessed for Leishmania infantum (L. infantum) serologically using western blot (WB) and molecularly using polymerase chain reaction (PCR). We also tested the association of Leishmania infection with some infectious agents like haemotropic Mycoplasma, Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV) together with the hematobiochemical status of the examined animals. Interestingly, all tested animals were asymptomatic and out of 250 examined serum samples, 33 (13.20%) samples (confidence interval (CI) 95% 9.56–17.96%) were positive at WB for L. infantum, whereas of the 282 blood samples, 80 (28.36%) returned a positive PCR (CI 95% 23.43–33.89%). Furthermore, there was a statistical association between PCR positivity for L. infantum and some hematological parameters besides FIV infection as well as a direct significant correlation between Mycoplasma infection and WB positivity. Taken together, the present findings report high prevalence of L. infantum among cats, which reinforces the significance of such positive asymptomatic animals and confirms the very low humoral response in this species. In addition, the laboratory values provide evidence that infection by the parasite is linked to alteration of some hematological parameters and is correlated to some infectious agents. These data are of interest and suggest future research for accurate diagnosis of such zoonosis.