bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of age, body height, body weight, body mass index and handgrip strength on the trajectory of the plantar pressure stance-phase curve of the gait cycle
Authors: Wolff Christian , Steinheimer Patrick , Warmerdam Elke , Dahmen Tim , Slusallek Philipp , Schlinkmann Christian , Chen Fei , Orth Marcel , Pohlemann Tim , Ganse Bergita
Published: February 2023

The analysis of gait patterns and plantar pressure distributions via insoles is increasingly used to monitor patients and treatment progress, such as recovery after surgeries. Despite the popularity of pedography, also known as baropodography, characteristic effects of anthropometric and other individual parameters on the trajectory of the stance phase curve of the gait cycle have not been previously reported. We hypothesized characteristic changes of age, body height, body weight, body mass index and handgrip strength on the plantar pressure curve trajectory during gait in healthy participants. Thirty-seven healthy women and men with an average age of 43.65 ± 17.59 years were fitted with Moticon OpenGO insoles equipped with 16 pressure sensors each. Data were recorded at a frequency of 100 Hz during walking at 4 km/h on a level treadmill for 1 minute. Data were processed via a custom-made step detection algorithm. The loading and unloading slopes as well as force extrema-based parameters were computed and characteristic correlations with the targeted parameters were identified via multiple linear regression analysis. Age showed a negative correlation with the mean loading slope. Body height correlated with Fmeanload and the loading slope. Body weight and the body mass index correlated with all analyzed parameters, except the loading slope. In addition, handgrip strength correlated with changes in the second half of the stance phase and did not affect the first half, which is likely due to stronger kick-off. However, only up to 46% of the variability can be explained by age, body weight, height, body mass index and hand grip strength. Thus, further factors must affect the trajectory of the gait cycle curve that were not considered in the present analysis. In conclusion, all analyzed measures affect the trajectory of the stance phase curve. When analyzing insole data, it might be useful to correct for the factors that were identified by using the regression coefficients presented in this paper.
Functional chitosan gel coating enhances antimicrobial properties and osteogenesis of titanium alloy under persistent chronic inflammation
Authors: Zhang Ti , Qin Xiaoyan , Gao Yuan , Kong Dan , Jiang Yuheng , Cui Xiang , Guo Miantong , Chen Junyu , Chang Feifan , Zhang Ming , Li Jia , Yin Pengbin
Published: February 2023

Titanium is widely used as surgical bone implants due to its excellent mechanical properties, corrosion resistance, and good biocompatibility. However, due to chronic inflammation and bacterial infections caused by titanium implants, they are still at risk of failure in interfacial integration of bone implants, severely limiting their broad clinical application. In this work, chitosan gels crosslinked with glutaraldehyde were prepared and successfully loaded with silver nanoparticles (nAg) and catalase nanocapsules (n (CAT)) to achieve functionalized coating on the surface of titanium alloy steel plates. Under chronic inflammatory conditions, n (CAT) significantly reduced the expression of macrophage tumor necrosis factor (TNF-α), increased the expression of osteoblast alkaline phosphatase (ALP) and osteopontin (OPN), and enhanced osteogenesis. At the same time, nAg inhibited the growth of S. aureus and E. coli. This work provides a general approach to functional coating of titanium alloy implants and other scaffolding materials.
Stem cell-derived extracellular vesicles: A novel and potential remedy for primary ovarian insufficiency
Authors: Geng Zixiang , Guo Hailing , Li Yifei , Liu Ying , Zhao Yongfang
Published: February 2023

Primary ovarian insufficiency (POI) is an essential cause of young female fertility loss. At present, there are many treatments for primary ovarian insufficiency, but due to the complexity of the pathogenesis of primary ovarian insufficiency, the efficacy still could not be satisfactory. Stem cell transplantation is a feasible intervention protocol for primary ovarian insufficiency. However, its wide application in the clinic is limited by some defects such as tumorigenic and controversial ethical issues. Stem cell-derived extracellular vesicles (EVs) represent an important mode of intercellular communication attracting increasing interest. It is well documented that stem cell-derived extracellular vesicles for primary ovarian insufficiency with exciting therapeutic effects. Studies have found that stem cell-derived extracellular vesicles could improve ovarian reserve, increase the growth of follicles, reduce follicle atresia, and restore hormone levels of FSH and E2. Its mechanisms include inhibiting ovarian granulosa cells (GCs) apoptosis, reactive oxygen species, and inflammatory response and promoting granulosa cells proliferation and angiogenesis. Thus, stem cell-derived extracellular vesicles are a promising and potential method for primary ovarian insufficiency patients. However, stem cell-derived extracellular vesicles are still a long way from clinical translation. This review will provide an overview of the role and the mechanisms of stem cell-derived extracellular vesicles in primary ovarian insufficiency, and further elaborate on the current challenges. It may suggest new directions for future research.
Phosphorylation of the actin-binding protein profilin2a at S137 modulates bidirectional structural plasticity at dendritic spines
Authors: Cornelius Jonas , Haak Stefan , Rothkegel Martin , Korte Martin , Michaelsen-Preusse Kristin
Published: February 2023

Background: Synaptic plasticity requires constant adaptation of functional and structural features at individual synaptic connections. Rapid re-modulation of the synaptic actin cytoskeleton provides the scaffold orchestrating both morphological and functional modifications. A major regulator of actin polymerization not only in neurons but also in various other cell types is the actin-binding protein profilin. While profilin is known to mediate the ADP to ATP exchange at actin monomers through its direct interaction with G-actin, it additionally is able to influence actin dynamics by binding to membrane-bound phospholipids as phosphatidylinositol (4,5)-bisphosphate (PIP2) as well as several other proteins containing poly-L-proline motifs including actin modulators like Ena/VASP, WAVE/WASP or formins. Notably, these interactions are proposed to be mediated by a fine-tuned regulation of post-translational phosphorylation of profilin. However, while phosphorylation sites of the ubiquitously expressed isoform profilin1 have been described and analyzed previously, there is still only little known about the phosphorylation of the profilin2a isoform predominantly expressed in neurons.

Methods: Here, utilizing a knock-down/knock-in approach, we replaced endogenously expressed profilin2a by (de)phospho-mutants of S137 known to alter actin-, PIP2 and PLP-binding properties of profilin2a and analyzed their effect on general actin dynamics as well as activity-dependent structural plasticity.

Results and Discussion: Our findings suggest that a precisely timed regulation of profilin2a phosphorylation at S137 is needed to mediate actin dynamics and structural plasticity bidirectionally during long-term potentiation and long-term depression, respectively.
Predictive value of remnant cholesterol level for all-cause mortality in heart failure patients
Authors: Zhao Lang , Zhao Xuemei , Tian Pengchao , Liang Lin , Huang Boping , Huang Liyan , Feng Jiayu , Zhang Yuhui , Zhang Jian
Published: February 2023

Background

Lower cholesterol levels are associated with increased mortality in heart failure (HF) patients. Remnant cholesterol corresponds to all cholesterol not found in high-density lipoprotein (HDL) and low-density lipoprotein (LDL). The prognostic role of remnant cholesterol in HF remains unknown.

Objective

To reveal the relationship between the baseline remnant cholesterol level and all-cause mortality in HF patients.

Methods

This study enrolled 2,823 patients hospitalized for HF. Kaplan–Meier analysis, Cox regression, C-statistic, net reclassification improvement (NRI), and integrated discrimination improvement (IDI) were used to evaluate the prognostic value of remnant cholesterol for all-cause mortality in HF.

Results

The mortality rate was lowest in the fourth quartile of remnant cholesterol, which had an adjusted hazard ratio (HR) for death of 0.56 [HR: 0.39, 95% confidence interval (CI): 0.46–0.68, p < 0.001] relative to the first quartile. After adjustment, a one-unit increase in the level of remnant cholesterol was associated with a 41% decrease in the risk of all-cause mortality (HR: 0.59, 95% CI: 0.47–0.73, p < 0.001). A refinement in risk prediction was observed after adding remnant cholesterol quartile to the original model (ΔC-statistic = 0.010, 95% CI: 0.003–0.017; NRI = 0.036, 95% CI: 0.003–0.070; IDI = 0.025, 95% CI: 0.018–0.033; all p < 0.05).

Conclusion

Low remnant cholesterol levels are associated with increased all-cause mortality in HF patients. The addition of the remnant cholesterol quartile improved the predictive value over traditional risk factors.

Clinical Trial Registration

ClinicalTrials.gov, Unique Identifier: NCT02664818.
General practitioners’ perceptions on opportunistic single-time point screening for atrial fibrillation: A European quantitative survey
Authors: Vermunicht Paulien , Grecu Mihaela , Deharo Jean-Claude , Buckley Claire M. , Palà Elena , Mairesse Georges H. , Farkowski Michal M. , Bergonti Marco , Pürerfellner Helmut , Hanson Coral L. , Neubeck Lis , Freedman Ben , Witt Henning , Hills Mellanie T. , Lund Jenny , Giskes Katrina , Engler Daniel , Schnabel Renate B. , Heidbuchel Hein , Desteghe Lien ,   for the AFFECT-EU investigators
Published: February 2023

Background

There is no clear guidance on how to implement opportunistic atrial fibrillation (AF) screening in daily clinical practice.

Objectives

This study evaluated the perception of general practitioners (GPs) about value and practicalities of implementing screening for AF, focusing on opportunistic single-time point screening with a single-lead electrocardiogram (ECG) device.

Methods

A descriptive cross-sectional study was conducted with a survey developed to assess overall perception concerning AF screening, feasibility of opportunistic single-lead ECG screening and implementation requirements and barriers.

Results

A total of 659 responses were collected (36.1% Eastern, 33.4% Western, 12.1% Southern, 10.0% Northern Europe, 8.3% United Kingdom & Ireland). The perceived need for standardized AF screening was rated as 82.7 on a scale from 0 to 100. The vast majority (88.0%) indicated no AF screening program is established in their region. Three out of four GPs (72.1%, lowest in Eastern and Southern Europe) were equipped with a 12-lead ECG, while a single-lead ECG was less common (10.8%, highest in United Kingdom & Ireland). Three in five GPs (59.3%) feel confident ruling out AF on a single-lead ECG strip. Assistance through more education (28.7%) and a tele-healthcare service offering advice on ambiguous tracings (25.2%) would be helpful. Preferred strategies to overcome barriers like insufficient (qualified) staff, included integrating AF screening with other healthcare programs (24.9%) and algorithms to identify patients most suitable for AF screening (24.3%).

Conclusion

GPs perceive a strong need for a standardized AF screening approach. Additional resources may be required to have it widely adopted into clinical practice.
The pre-ejection period is a highly stress dependent parameter of paramount importance for pulse-wave-velocity based applications
Authors: Pilz Niklas , Patzak Andreas , Bothe Tomas L.
Published: February 2023

Purpose

The pulse-wave-velocity, is used for indirect, cuff-less, continuous blood pressure estimation. It is commonly detected by measuring the time delay between a defined point in an ECG and the arrival of the peripheral pulse wave (e.g., oxygen saturation sensor). The period between electrical stimulation of the heart (ECG) and actual blood ejection from the heart is called the pre-ejection period (PEP). This study aims at characterizing the PEP under mental and physical stress with focus on its relations to other cardiovascular parameters such as heart rate and importance for blood pressure (BP) estimation.

Methods

We measured the PEP in 71 young adults at rest, under mental (TSST) and physical stress (ergometer) via impedance-cardiography.

Results

The PEP is highly dependent on mental and physical load. It is strongly correlated with indicators of sympathetic strain (p < 0.001). At rest (mean 104.5 ms), the PEP shows a high interindividual variability but small intraindividual variability. Mental stress decreases the PEP by 16% (mean 90.0 ms) while physical stress halves PEP (mean 53.9 ms). The PEP does correlate differently with heart rate under differing circumstances (rest: R2 0.06, mental stress: R2 0.29, physical stress: R2 0.65). Subsequently, using PEP and heart rate enables the discrimination of rest, mental and physical strain with a positive predictive value of 93%.

Conclusion

The PEP is a cardiovascular parameter with large interindividual variability at rest and subject-depended dynamic under load which is of great importance for ECG-based pulse-wave-velocity (PWV) determination. Considering its variability and large impact on the pulse arrival time, PEP is a crucial factor in PWV based BP estimation.
Statistical evaluation of earthquake forecast efficiency using earthquake-catalog and fault slip rate in the Sichuan-Yunnan region, China
Authors: Zhang Bo , Chen Shi , Zhuang Jiancang , Zhang Bei , Wu Xu , Liang Baojuan
Published: February 2023

Epicenter locations are generally adjacent to active faults and past seismicity regions. Past earthquake catalogs and the geometry of the active faults can provide key prior knowledge concerning earthquake forecasts. In this study, we first introduce two straightforward dedicated models, the proximity-to-past-earthquakes (PPE) and proximity-to-mapped-faults (PMF) models, to fit the seismicity in the Sichuan-Yunnan region, China. The hybrid proximity-to-known-sources (PKS) model with the optimized model parameters is then used to estimate the probability of earthquake occurrence. Second, to compare the PKS forecast efficiency to those of different models, retrospective tests are applied to a dataset located in the Sichuan-Yunnan region. The results show that the probability maps derived from PPE, PMF, and PKS have non-uniform Poisson distribution features and that there is forecasting significance for past cases of moderate earthquakes in the test region. Finally, using Molchan error diagram tests, we find that the hybrid PKS model performs better than the other models in the testing region. The unsatisfactory performance of the PMF model for earthquake forecasting may lie both in the incompleteness of the fault database and the lack of consideration of co-seismic ruptures. Therefore, one of the three models can be used as a base model for comparing and evaluating earthquake forecast strategies.
Diagnostic efficiency of existing guidelines and the AI-SONIC™ artificial intelligence for ultrasound-based risk assessment of thyroid nodules
Authors: Yang Linxin , Lin Ning , Wang Mingyan , Chen Gaofang
Published: February 2023

Introduction

The thyroid ultrasound guidelines include the American College of Radiology Thyroid Imaging Reporting and Data System, Chinese-Thyroid Imaging Reporting and Data System, Korean Society of Thyroid Radiology, European-Thyroid Imaging Reporting and Data System, American Thyroid Association, and American Association of Clinical Endocrinologists/American College of Endocrinology/Associazione Medici Endocrinologi guidelines. This study aimed to compare the efficiency of the six ultrasound guidelines vs. an artificial intelligence system (AI-SONICTM) in differentiating thyroid nodules, especially medullary thyroid carcinoma.

Methods

This retrospective study included patients with medullary thyroid carcinoma, papillary thyroid carcinoma, or benign nodules who underwent nodule resection between May 2010 and April 2020 at one hospital. The diagnostic efficacy of the seven diagnostic tools was evaluated using the receiver operator characteristic curves.

Results

Finally, 432 patients with 450 nodules were included for analysis. The American Association of Clinical Endocrinologists/American College of Endocrinology/Associazione Medici Endocrinologi guidelines had the best sensitivity (88.1%) and negative predictive value (78.6%) for differentiating papillary thyroid carcinoma or medullary thyroid carcinoma vs. benign nodules, while the Korean Society of Thyroid Radiology guidelines had the best specificity (85.6%) and positive predictive value (89.6%), and the American Thyroid Association guidelines had the best accuracy (83.7%). When assessing medullary thyroid carcinoma, the American Thyroid Association guidelines had the highest area under the curve (0.78), the American College of Radiology Thyroid Imaging Reporting and Data System guidelines had the best sensitivity (90.2%), and negative predictive value (91.8%), and AI-SONICTM had the best specificity (85.6%) and positive predictive value (67.5%). The Chinese-Thyroid Imaging Reporting and Data System guidelines had the best under the curve (0.86) in diagnosing malignant tumors vs. benign tumors, followed by the American Thyroid Association and Korean Society of Thyroid Radiology guidelines. The best positive likelihood ratios were achieved by the Korean Society of Thyroid Radiology guidelines and AI-SONICTM (both 5.37). The best negative likelihood ratio was achieved by the American Association of Clinical Endocrinologists/American College of Endocrinology/Associazione Medici Endocrinologi guidelines (0.17). The highest diagnostic odds ratio was achieved by the American Thyroid Association guidelines (24.78).

Discussion

All six guidelines and the AI-SONICTM system had satisfactory value in differentiating benign vs. malignant thyroid nodules.
Multi-resolution based PID controller for frequency regulation of a hybrid power system with multiple interconnected systems
Authors: Zhang Peng , Daraz Amil , Malik Suheel Abdullah , Sun Chao , Basit Abdul , Zhang Guoqiang
Published: February 2023

Automatic generation control (AGC) in modern power systems (PS) is difficult because the output power of many power resources is intermittent, and the load and system parameters vary widely. In this paper, a novel control scheme known as the wavelet based multiresolution proportional integral derivative (MRPID) controller for multiple interconnected hybrid power sources is presented. The discrete wavelet transform (DWT) is used in the proposed wavelet based MRPID controller to split the error between the actual and target responses into different frequency components at several stages. To ensure optimum system performance, the gains of the MRPID controller are fine-tuned using the Fox Optimizer Algorithm (FOA), a new powerful metaheuristic technique. The proposed MRPID controller is evaluated in a three-area hybrid system where each area contains a combination of conventional generation (gas, thermal reheat and hydro) and renewable generation sources (solar, and wind). The proposed controller also accounts for system non-linearities, including boiler dynamics, time delay, dead band, generation rate limitation, system uncertainties, and load changes. In the hybrid system studied, the proposed MRPID is compared with FOA-tuned PID and PI controllers. The proposed MRPID controller tuned with FOA algorithm effectively reducing the peak overshoot of 89.03%, 76.89 and 56.96% and undershoot of 69.52%,66.90 and 94.29% for ∆Ptie12, ∆Ptie23 and ∆Ptie13 respectively as compared to FOA based PI controller.
Heat transfer of supercritical pressure water in helical tubes
Authors: Liu Mengmeng , Zhang Zhen , Yang Xingtuan , Jiang Shengyao
Published: February 2023

Subcritical steam generators in the current high-temperature gas-cooled reactor pebble-bed module in China can be replaced by supercritical steam generators to better coordinate the reactors and supercritical steam turbine unit to improve the thermal efficiency with no fluid phase change at supercritical pressures. The heat transfer of supercritical-pressure water flow in helical tubes with various helix diameters was simulated using the re-normalization group k–ε model to investigate the effects of finite-thickness walls, inlet mass flux, and helix diameter. The finite-thickness wall barely affected local heat transfer in the straight tube with inlet mass flux of 1,260 kg/(m2 s) whereas the local heat transfer in the helical tube was very different with relative difference in q/qav between the inside and bottom, caused by the centrifugal force, is 36%. In helical tubes, the heat transfer coefficients in the inner bar were the lowest, with a low fluid velocity under the effect of the centrifugal force. The buoyancy and centrifugal force in the helical tubes caused non-uniformity in the wall temperature; however, when the fluid temperature increased below the pseudo-critical temperature, the temperature distributions showed an increasing trend. The non-uniformities in the wall temperature distributions decreased as the centrifugal force increased, and they were suppressed by the finite-thickness wall.
Citizen science interactions with official geospatial information; Case studies from Mexico
Authors: Jacquin Céline , Merodio Gómez Paloma , Arriaga Vivian , Santiago Andrea
Published: February 2023

This paper presents an overview of the integration of participatory processes in the production of official data. Through a series of interviews with strategic stakeholders we identified the key elements to institutionalize citizen science in the production of geospatial information. This article discusses practical contexts of uses of data produced or complemented by citizens in Mexico. We analyze institutional processes that facilitates or make difficult the integration into official mechanisms for generating more accurate cartographic information in various institutions, focusing on its possible adoption, in particular by the National Institute of Statistics and Geography (INEGI) of Mexico. Resources, data integration models, workflows, and an organizational structure are needed to benefit from citizen science. We find that the adoption of citizen science within an organization is subject to a well-defined and structured interest driven by leadership and implemented collectively. This presents a paradigm shift in obtaining information, citizen science as official data through concrete and functional information products will allow end users to benefit from timely and accurate data. The purpose of this article is then to generate organizational knowledge on how to use citizen science in public institutions, with long-term perspective, to mediate the lack of current and accurate spatial data and participate in social innovation.
Development of a biotic integrity index based on long-term fish assemblage changes after dam construction in China
Authors: Zhu Di , Yang Zhi , Chen Xiaojuan , Jin Yao , Li Dewang
Published: February 2023

Multi-metric indicators based on fish assemblages have been widely applied to assess aquatic ecosystem biotic integrity. However, many of these were not detailed or standardized in metrics selection and reference status for a reservoir ecosystem under the long-term effects of hydro-electrical dam development. This makes it very difficult to determine the reference status and metrics because fish assemblages have been affected by human activities such as large reservoir operations. Based on long-term fish assemblage data, there were significant differences in fish composition between backwater and reservoir areas 10 years after the Three Gorges Reservoir (TGR) formation in China. We conducted this study to develop a new fish index of biotic integrity (IBI) to evaluate a large river reservoir ecosystem based on long-term monitoring data by establishing subregional habitat reference and selecting sensitive and concise metrics. In the Three Gorges Reservoir, we found that: 1) dynamic change is an important feature of fish assemblages in a relatively dynamic state after a decade of intensive disturbance caused by reservoir operations; 2) reference conditions for the reservoir evaluation should be selected in different stages for two different ecological habitats (lotic backwater and lentic reservoir reaches); 3) two core metrics and five sensitive metrics were selected to reflect fish assemblage degradation based on long term observations; and 4) overall fish biological integrity is generally “fair-poor,” in the reservoir area it is “fair” and “poor,” and in the reservoir lotic backwater it is “fair.” The long-term effects of large canyon reservoirs on biological integrity require long-term observation, and reasonable recovery targets should be set at different stages in reservoir management. In this study, a new biological integrity index was developed to screen and evaluate the ecological status of a semi-artificial reservoir ecosystem. Our results support reservoir biologically integrated management and green sustainable development goals.
Subtyping based on immune cell fractions reveal heterogeneity of cardiac fibrosis in end-stage heart failure
Authors: Zou Shangjie , Khoo Bee Luan
Published: February 2023

Background

A central issue hindering the development of effective anti-fibrosis drugs for heart failure is the unclear interrelationship between fibrosis and the immune cells. This study aims at providing precise subtyping of heart failure based on immune cell fractions, elaborating their differences in fibrotic mechanisms, and proposing a biomarker panel for evaluating intrinsic features of patients’ physiological statuses through subtype classification, thereby promoting the precision medicine for cardiac fibrosis.

Methods

We inferred immune cell type abundance of the ventricular samples by a computational method (CIBERSORTx) based on ventricular tissue samples from 103 patients with heart failure, and applied K-means clustering to divide patients into two subtypes based on their immune cell type abundance. We also designed a novel analytic strategy: Large-Scale Functional Score and Association Analysis (LAFSAA), to study fibrotic mechanisms in the two subtypes.

Results

Two subtypes of immune cell fractions: pro-inflammatory and pro-remodeling subtypes, were identified. LAFSAA identified 11 subtype-specific pro-fibrotic functional gene sets as the basis for personalised targeted treatments. Based on feature selection, a 30-gene biomarker panel (ImmunCard30) established for diagnosing patient subtypes achieved high classification performance, with the area under the receiver operator characteristic curve corresponding to 0.954 and 0.803 for the discovery and validation sets, respectively.

Conclusion

Patients with the two subtypes of cardiac immune cell fractions were likely having different fibrotic mechanisms. Patients’ subtypes can be predicted based on the ImmunCard30 biomarker panel. We envision that our unique stratification strategy revealed in this study will unravel advance diagnostic techniques for personalised anti-fibrotic therapy.
CD83 expressed by macrophages is an important immune checkpoint molecule for the resolution of inflammation
Authors: Peckert-Maier Katrin , Langguth Pia , Strack Astrid , Stich Lena , Mühl-Zürbes Petra , Kuhnt Christine , Drassner Christina , Zinser Elisabeth , Wrage Marius , Mattner Jochen , Steinkasserer Alexander , Royzman Dmytro , Wild Andreas B.
Published: February 2023

Excessive macrophage (Mφ) activation results in chronic inflammatory responses or autoimmune diseases. Therefore, identification of novel immune checkpoints on Mφ, which contribute to resolution of inflammation, is crucial for the development of new therapeutic agents. Herein, we identify CD83 as a marker for IL-4 stimulated pro-resolving alternatively activated Mφ (AAM). Using a conditional KO mouse (cKO), we show that CD83 is important for the phenotype and function of pro-resolving Mφ. CD83-deletion in IL-4 stimulated Mφ results in decreased levels of inhibitory receptors, such as CD200R and MSR-1, which correlates with a reduced phagocytic capacity. In addition, CD83-deficient Mφ upon IL-4 stimulation, show an altered STAT-6 phosphorylation pattern, which is characterized by reduced pSTAT-6 levels and expression of the target gene Gata3. Concomitantly, functional studies in IL-4 stimulated CD83 KO Mφ reveal an increased production of pro-inflammatory mediators, such as TNF-α, IL-6, CXCL1 and G-CSF. Furthermore, we show that CD83-deficient Mφ have enhanced capacities to stimulate the proliferation of allo-reactive T cells, which was accompanied by reduced frequencies of Tregs. In addition, we show that CD83 expressed by Mφ is important to limit the inflammatory phase using a full-thickness excision wound healing model, since inflammatory transcripts (e.g. Cxcl1, Il6) were increased, whilst resolving transcripts (e.g. Ym1, Cd200r, Msr-1) were decreased in wounds at day 3 after wound infliction, which reflects the CD83 resolving function on Mφ also in vivo. Consequently, this enhanced inflammatory milieu led to an altered tissue reconstitution after wound infliction. Thus, our data provide evidence that CD83 acts as a gatekeeper for the phenotype and function of pro-resolving Mφ.
Differences of gut microbiota and behavioral symptoms between two subgroups of autistic children based on γδT cells-derived IFN-γ Levels: A preliminary study
Authors: Xu Xin-Jie , Lang Ji-Dong , Yang Jun , Long Bo , Liu Xu-Dong , Zeng Xiao-Feng , Tian Geng , You Xin
Published: February 2023

Background

Autism Spectrum Disorders (ASD) are defined as a group of pervasive neurodevelopmental disorders, and the heterogeneity in the symptomology and etiology of ASD has long been recognized. Altered immune function and gut microbiota have been found in ASD populations. Immune dysfunction has been hypothesized to involve in the pathophysiology of a subtype of ASD.

Methods

A cohort of 105 ASD children were recruited and grouped based on IFN-γ levels derived from ex vivo stimulated γδT cells. Fecal samples were collected and analyzed with a metagenomic approach. Comparison of autistic symptoms and gut microbiota composition was made between subgroups. Enriched KEGG orthologues markers and pathogen-host interactions based on metagenome were also analyzed to reveal the differences in functional features.

Results

The autistic behavioral symptoms were more severe for children in the IFN-γ-high group, especially in the body and object use, social and self-help, and expressive language performance domains. LEfSe analysis of gut microbiota revealed an overrepresentation of Selenomonadales, Negatiyicutes, Veillonellaceae and Verrucomicrobiaceae and underrepresentation of Bacteroides xylanisolvens and Bifidobacterium longum in children with higher IFN-γ level. Decreased metabolism function of carbohydrate, amino acid and lipid in gut microbiota were found in the IFN-γ-high group. Additional functional profiles analyses revealed significant differences in the abundances of genes encoding carbohydrate-active enzymes between the two groups. And enriched phenotypes related to infection and gastroenteritis and underrepresentation of one gut–brain module associated with histamine degradation were also found in the IFN-γ-High group. Results of multivariate analyses revealed relatively good separation between the two groups.

Conclusions

Levels of IFN-γ derived from γδT cell could serve as one of the potential candidate biomarkers to subtype ASD individuals to reduce the heterogeneity associated with ASD and produce subgroups which are more likely to share a more similar phenotype and etiology. A better understanding of the associations among immune function, gut microbiota composition and metabolism abnormalities in ASD would facilitate the development of individualized biomedical treatment for this complex neurodevelopmental disorder.
Mobilizing the fishing industry to address data gaps created by shifting species distribution
Authors: Heimann Thomas , Verkamp Hannah , McNamee Jason , Bethoney N. David
Published: February 2023

Globally, climate change is inducing range shifts and expansions for numerous species. For commercially exploited species, such as those in directed fisheries, this can cause numerous issues with management and jurisdictions as the species shift and expand into areas at levels previously unseen. The black sea bass (Centropristis striata) fishery has rapidly expanded in the northern Atlantic. Over the past decade commercial landings have more than doubled in the New England and Mid-Atlantic regions. This increase is related to a northward shift in the species’ center of biomass and range expansion. There is a crucial need for increased data in this species’ northern range. Oftentimes, large-scale fisheries data collection is limited by available resources and the difficulty of collecting data at-sea. Citizen science, such as fishing industry-based Research Fleets, represent a cost-effective option to help overcome these limitations and allow for the rapid collection of large amounts of data. The Commercial Fisheries Research Foundation and Rhode Island Department of Environmental Management established the Black Sea Bass Research Fleet in 2016. The Research Fleet is composed of fishers representing a variety of gear types who collect fishery-dependent data on black sea bass at-sea on a custom tablet application. In five years, 20 captains participated in the Research Fleet and collected length, visually-identified sex, and disposition data on 40,939 individual black sea bass throughout southern New England and into the Mid-Atlantic Bight. Catch, effort, and basic environmental data from 2,288 sampling sessions have been collected alongside this biological data. We apply the collaborative Research Fleet approach to a finfish for the first time and evaluate its performance over the first five years of sampling through participant engagement, magnitude of data collection, and interest in collected data. Further, we introduce the next steps being undertaken to incorporate the collected data into the management framework. This project illustrates that a science-industry research collaboration such as the Black Sea Bass Research Fleet can consistently collect large amounts of fishery-dependent data on black sea bass, and highlights a mutual interest among fishers, scientists, and managers to expand the collection of reliable data on this important species.
Review on the physical chemistry of iodine transformations in the oceans
Authors: Luther George W.
Published: February 2023

The transformation between iodate (IO3−), the thermodynamically stable form of iodine, and iodide (I-), the kinetically stable form of iodine, has received much attention because these species are often dependent on the oxygen concentration, which ranges from saturation to non-detectable in the ocean. As suboxic conditions in the ocean’s major oxygen minimum zones indicate that IO3− is minimal or non-detectable, the incorporation of IO3− into carbonate minerals has been used as a redox proxy to determine the O2 state of the ocean. Here, I look at the one and two electron transfers between iodine species with a variety of oxidants and reductants to show thermodynamics of these transformations. The IO3− to IO2− conversion is shown to be the controlling step in the reduction reaction sequence due to thermodynamic considerations. As IO3− reduction to IO2− is more favorable than NO3− reduction to NO2− at oceanic pH values, there is no need for nitrate reductase for IO3− reduction as other reductants (e.g. Fe2+, Mn2+) and dissimilatory IO3− reduction by microbes during organic matter decomposition can affect the transformation. Unfortunately, there is a dearth of information on the kinetics of reductants with IO3−; thus, the thermodynamic calculations suggest avenues for research. Conversely, there is significant information on the kinetics of I- oxidation with various oxygen species. In the environment, I- oxidation is the controlling step for oxidation. The oxidants that can lead to IO3− are reactive oxygen species with O3 and •OH being the most potent as well as sedimentary oxidized Mn, which occurs at lower pH than ocean waters. Recent work has shown that iodide oxidizing bacteria can also form IO3−. I- oxidation is more facile at the sea surface microlayer and in the atmosphere due to O3.
Anemia is independently associated with mortality in people living with human immunodeficiency virus/acquired immune deficiency syndrome: A propensity score matching-based retrospective cohort study in China
Authors: Jin Meihua , Wang Yanan , Li Jing , Wu Zhenqian , Liu Xiaoqi , Wang Hui , Chen Yuxin , Wang Ziyi , Tong Zhaowei , Li Xiaofeng , Ren Feilin , Zhu Xiaojuan , Yang Zhongrong , Mao Guangyun
Published: February 2023

Although previous studies have suggested that hemoglobin is related to the health status of people living with human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) (PLWHA), the role of anemia in mortality remains unclear. This study aimed to comprehensively quantify the effect of anemia on the mortality risk of PLWHA. In this retrospective cohort study, we thoroughly estimated the effect of anemia on PLWHA mortality, using data collected from January 2005 to June 2022 in the Huzhou area, in 450 subjects extracted from the database of the China Disease Prevention and Control Information System and matched them using a propensity score matching approach to balance potential confounding bias. The potential exposure–response relationship between anemia, hemoglobin concentration, and the mortality of PLWHA was also carefully estimated. A series of subgroup analyses, including interaction analysis, was further conducted to validate the robustness of the effect of anemia on PLWHA death risk. Anemia was significantly associated with an elevated death risk in PLWHA, with an increase of 74% (adjusted hazard ratio [AHR]: 1.74; 95% confidence interval [CI]: 1.03–2.93; p = 0.038) in those with anemia after adjusting for potential confounders. PLWHA with moderate or severe anemia had a higher risk of death, with an 86% increase (AHR = 1.86; 95% CI: 1.01–3.42; p = 0.045). Meanwhile, the AHR tended to increase by 85% on average (AHR = 1.85, 95% CI: 1.37–2.50; p < 0.001) with a per standard deviation (SD) decrease in plasma hemoglobin. Consistent relationships between plasma hemoglobin and the risk of death were further observed in the results from multiple quantile regression models, restricted cubic spline regression models, and a series of subgroup analyses. Anemia is an independent risk factor for HIV/AIDS-related mortality. Our findings may provide new insights into the relevance of PLWHA administration to public health policy, which demonstrate that this low-cost and routinely measured marker (hemoglobin) can be a marker of poor prognosis even before the start of HAART.
Phosphorylation status of a conserved residue in the adenylate cyclase of Botrytis cinerea is involved in regulating photomorphogenesis, circadian rhythm, and pathogenicity
Authors: Cai Yunfei , Chen Xue , Li Peixuan , Ren Weiheng , Zhang Qiang , Wang Yiwen , Jiang Yina , Zhu Pinkuan , Toyoda Hideyoshi , Xu Ling
Published: February 2023

Adenylate cyclase (AC) regulates growth, reproduction, and pathogenicity in many fungi by synthesizing cyclic adenosine monophosphate (cAMP) and activating downstream protein kinase A (PKA). Botrytis cinerea is a typical necrotrophic plant-pathogenic fungus. It shows a typical photomorphogenic phenotype of conidiation under light and sclerotia formation under dark; both are important reproduction structures for the dispersal and stress resistance of the fungus. The report of B. cinerea adenylate cyclase (BAC) mutation showed it affects the production of conidia and sclerotia. However, the regulatory mechanisms of the cAMP signaling pathways in photomorphogenesis have not been clarified. In this study, the S1407 site was proven to be an important conserved residue in the PP2C domain which poses a remarkable impact on the phosphorylation levels and enzyme activity of the BAC and the overall phosphorylation status of total proteins. The point mutation bacS1407P, complementation bacP1407S, phosphomimetic mutation bacS1407D, and phosphodeficient mutation bacS1407A strains were used for comparison with the light receptor white-collar mutant Δbcwcl1 to elucidate the relationship between the cAMP signaling pathway and the light response. The comparison of photomorphogenesis and pathogenicity phenotype, evaluation of circadian clock components, and expression analysis of light response transcription factor genes Bcltf1, Bcltf2, and Bcltf3 showed that the cAMP signaling pathway could stabilize the circadian rhythm that is associated with pathogenicity, conidiation, and sclerotium production. Collectively, this reveals that the conserved S1407 residue of BAC is a vital phosphorylation site to regulate the cAMP signaling pathway and affects the photomorphogenesis, circadian rhythm, and pathogenicity of B. cinerea.
Soluble peptidoglycan fragments produced by Limosilactobacillus fermentum with antiproliferative activity are suitable for potential therapeutic development: A preliminary report
Authors: Fuochi Virginia , Spampinato Mariarita , Distefano Alfio , Palmigiano Angelo , Garozzo Domenico , Zagni Chiara , Rescifina Antonio , Li Volti Giovanni , Furneri Pio Maria
Published: February 2023

Currently, the use of probiotic strains and their products represents a promising innovative approach as an antagonist treatment against many human diseases. Previous studies showed that a strain of Limosilactobacillus fermentum (LAC92), previously defined as Lactobacillus fermentum, exhibited a suitable amensalistic property. The present study aimed to purify the active components from LAC92 to evaluate the biological properties of soluble peptidoglycan fragments (SPFs). The cell-free supernatant (CFS) and bacterial cells were separated after 48 h of growth in MRS medium broth and treated for isolation of SPFs. Antimicrobial activity and proliferation analysis on the human cell line HTC116 were performed using technologies such as xCELLigence, count and viability, and clonogenic analysis. MALDI-MS investigation and docking analysis were performed to determine the molecular structure and hypothetical mode of action, respectively. Our results showed that the antimicrobial activity was mainly due to SPFs. Moreover, the results obtained when investigating the SPF effect on the cell line HCT116 showed substantial preliminary evidence, suggesting their significant cytostatic and quite antiproliferative properties. Although MALDI was unable to identify the molecular structure, it was subsequently revealed by analysis of the bacterial genome. The amino acid structure is called peptide 92. Furthermore, we confirmed by molecular docking studies the interaction of peptide 92 with MDM2 protein, the negative regulator of p53. This study showed that SPFs from the LAC92 strain exerted anticancer effects on the human colon cancer HCT116 cell line via antiproliferation and inducing apoptosis. These findings indicated that this probiotic strain might be a potential candidate for applications in functional products in the future. Further examination is needed to understand the specific advantages of this probiotic strain and improve its functional features to confirm these data. Moreover, deeper research on peptide 92 could increase our knowledge and help us understand if it will be possible to apply to specific diseases such as CRC.
Reinforcement learning based variable damping control of wearable robotic limbs for maintaining astronaut pose during extravehicular activity
Authors: Zhao Sikai , Zheng Tianjiao , Sui Dongbao , Zhao Jie , Zhu Yanhe
Published: February 2023

As astronauts perform on-orbit servicing of extravehicular activity (EVA) without the help of the space station’s robotic arms, it will be rather difficult and labor-consuming to maintain the appropriate position in case of impact. In order to solve this problem, we propose the development of a wearable robotic limb system for astronaut assistance and a variable damping control method for maintaining the astronaut’s position. The requirements of the astronaut’s impact-resisting ability during EVA were analyzed, including the capabilities of deviation resistance, fast return, oscillation resistance, and accurate return. To meet these needs, the system of the astronaut with robotic limbs was modeled and simplified. In combination with this simplified model and a reinforcement learning algorithm, a variable damping controller for the end of the robotic limb was obtained, which can regulate the dynamic performance of the robot end to resist oscillation after impact. A weightless simulation environment for the astronaut with robotic limbs was constructed. The simulation results demonstrate that the proposed method can meet the recommended requirements for maintaining an astronaut’s position during EVA. No matter how the damping coefficient was set, the fixed damping control method failed to meet all four requirements at the same time. In comparison to the fixed damping control method, the variable damping controller proposed in this paper fully satisfied all the impact-resisting requirements by itself. It could prevent excessive deviation from the original position and was able to achieve a fast return to the starting point. The maximum deviation displacement was reduced by 39.3% and the recovery time was cut by 17.7%. Besides, it also had the ability to prevent reciprocating oscillation and return to the original position accurately.
Regional variances depict a unique glial-specific inflammatory response following closed-head injury
Authors: White Michelle R. , VandeVord Pamela J.
Published: February 2023

Mild traumatic brain injuries (mTBI) constitute a significant health concern with clinical symptoms ranging from headaches to cognitive deficits. Despite the myriad of symptoms commonly reported following this injury, there is still a lack of knowledge on the various pathophysiological changes that occur. Preclinical studies are at the forefront of discovery delineating the changes that occur within this heterogeneous injury, with the emergence of translational models such as closed-head impact models allowing for further exploration of this injury mechanism. In the current study, male rats were subjected to a closed-head controlled cortical impact (cCCI), producing a concussion (mTBI). The pathological effects of this injury were then evaluated using immunoflourescence seven days following. The results exhibited a unique glial-specific inflammatory response, with both the ipsilateral and contralateral sides of the cortex and hippocampus showing pathological changes following impact. Overall these findings are consistent with glial changes reported following concussions and may contribute to subsequent symptoms.