bluuebunny/crossref_metadata_2025 huggingface dataset:
Adventitious root formation confers waterlogging tolerance in cowpea (Vigna unguiculata (L.) Walp.)
Authors: Basavaraj P. S. , Jangid Krishna Kumar , Babar Rohit , Gangana Gowdra Vinay M. , Gangurde Anuja , Shinde Shweta , Tripathi Kuldeep , Patil Deepak , Boraiah K. M. , Rane Jagadish , Harisha C. B. , Halli Hanamant , Sammi Reddy K. , Prabhakar M.
Published: April 2024

Crop adaptation to waterlogging stress necessitates alterations in their morpho-physiological and biochemical characteristics. Cowpeas, which serve as a dual-purpose legume crop (food and fodder), are sensitive to waterlogging stress, especially when exposed to extended periods of water stagnation during the early growth stage. In this study, we subjected five distinct and superior cowpea varieties to 10 days of waterlogging stress at the early seedling stage (V2, 15 days post emergence for 10 days) under controlled conditions. The aim was to comprehend the response of these varieties and identify the ideal trait for screening a large collection of cowpea genetic resources for waterlogging tolerance. We measured and analyzed changes in morpho-physiological and root parameters to gain a deeper understanding of the mechanism underlying waterlogging tolerance. The treatment (waterlogging and control), genotype, and their interactions had a significant impact on the most studied traits (p < 0.05). The results indicated a significant reduction in morpho-physiological parameters such as plant height, leaf area, leaf number, Normalized Difference Vegetation Index (NDVI), chlorophyll content, and chlorophyll fluorescence parameters under stress treatment than control conditions. However, root parameters like the number of adventitious roots (AR) and their length (ARL) significantly increased under waterlogging stress in tolerant cowpea varieties like DC15 and PL4. Correlation and PCA analyses further revealed a positive and significant association between cowpeas’ waterlogging tolerance and AR formation and its AR length. Therefore, the current study reveals that swift development of AR and ARL may serve as potential traits conferring waterlogging tolerance in cowpeas. Using suitable mapping populations, these traits could reveal genomic regions associated with waterlogging tolerance in cowpeas. The tolerant varieties and key traits identified in this study could be beneficial in breeding programs aimed at enhancing waterlogging tolerance in cowpeas.
Emerging Health Care Leaders: Lessons From a Novel Leadership and Community-Building Program
Authors: Martani Andrea , Ulyte Agne , Menges Dominik , Reeves Emily , Puhan Milo A. , Heusser Rolf
Published: April 2024

Background:

Although there are guidelines and ideas on how to improve public health education, translating innovative approaches into actual training programs remains challenging. In this article, we provide an overview of some initiatives that tried to put this into action in different parts of the world, and present the Emerging Health Care Leader (EHCL), a novel training program developed in Switzerland.

Policy Options and Recommendations:

Looking at the experience of the EHCL, we propose policymakers and other interested stakeholders who wish to help reform public health education to support these initiatives not only through funding, but by valuing them through the integration of early career healthcare leaders in projects where their developing expertise can be practically applied.

Conclusion:

By openly sharing the experiences, strengths, weaknesses, and lessons learned with the EHCL program, we aim to foster a transparent debate on how novel training programs in public health can be organised.
Strategic Networks’ Dynamics: Evidence of Member Firms’ Retention and Departures in Brazil
Authors: Braga Thais Guerra , Filho Nelson Casarotto , Wegner Douglas , Brambilla Flávio Régio , Ferreira Bruno Morgado
Published: April 2024

Strategic Networks (SNs) have emerged as a prevalent organizational form that offers synergistic benefits to participating firms. Despite their potential, SNs often experience member firms’ departure, raising concerns about their long-term viability. This study explores the factors that influence firms’ decisions to exit SNs by examining the interplay between network maturity, member firms’ satisfaction, and their individual characteristics. Using data from 338 Brazilian firms involved in SNs, a cluster analysis was employed to identify distinct groups of firms based on shared attributes. The findings revealed a significant association between higher perceived maturity, greater satisfaction with SNs, and lower likelihood of departure. These results suggest that network leaders should prioritize cultivating a mature and satisfying SN environment to mitigate member exits. By identifying the critical distinctions between the remaining and departing firms, this study provides valuable insights for network managers seeking to strengthen the longevity and stability of their strategic partnerships.
Comparison of Twenty Selected Fenugreek Genotypes Grown under Irrigated and Dryland Conditions: Morphology, Yield, Quality Properties and Antioxidant Activities
Authors: Camlica Mahmut , Yaldiz Gulsum
Published: April 2024

In this study, 18 fenugreek genotypes and two cultivars were cultivated under irrigated and dryland conditions to determine the morphological, yield, quality, and bioactive properties. The morphological and seed yield values showed differences among the fenugreek genotypes and cultivars. The PI 286532 and PI 639185 genotypes for total alkaloid content and the PI 426973 and PI 572538 genotypes for trigonelline content were prominent under both irrigated and dryland conditions. The PI 215615 and PI 286532 genotypes were found to be rich in fixed oil contents under two growing conditions, while all fenugreek genotypes had high linoleic contents. The PI 568215 and PI 251640 genotypes and the gürarslan cultivar had the highest antioxidant activity compared with the other genotypes. The PI 215615 and PI 302448 genotypes and the gürarslan cultivar were found to be superior with respect to phenolic and flavonoid contents. Generally, the cluster analysis clustered the genotypes into two main groups and two sub-groups. Group B included more than 66% of the genotypes and cultivars. The biplot analysis accounted for over 53% of total variations. As a result of this study, most of the genotypes were found to be high in the examined properties, and these genotypes were good sources of natural components with potential applications in the food and pharmaceutical industries.
Changes in Microbial Community and Activity of Chernozem Soil under Different Management Systems in a Long-Term Field Experiment in Hungary
Authors: Balla Kovács Andrea , Juhász Evelin Kármen , Béni Áron , Kincses Ida , Tállai Magdolna , Sándor Zsolt , Kátai János , Rátonyi Tamás , Kremper Rita
Published: April 2024

The effects of intensive and reduced tillage, fertilization, and irrigation on soil chemical and microbiological parameters were studied in a long-term field experiment in Hungary. The treatments were plowing tillage, ripper tillage, strip tillage; control (without fertilization), NPK fertilization (N: 160 kg/ha; P: 26 kg/ha; K: 74 kg/ha); and non-irrigation and irrigation. Soil samples were collected through maize monoculture in the fall of 2021 in the 30th year of the experiment. The soil organic carbon, total nitrogen, soil microbial biomass (based on PLFA analysis), and soil enzyme activity were observed to be significantly high in the strip tillage plots, but were lower in the ripper tillage plots, and even lower in the plowing tillage plots. The fungal, arbuscular mycorrhiza fungal, and bacterial biomasses were significantly higher in the strip tillage and ripper tillage plots compared to the plowing tillage plots. The strip tillage treatment was found to be the most favorable cultivation method for improving the microbial biomass and activity of Chernozem soil, followed by the ripper tillage and plowing tillage treatments. The long-term use of chemical fertilizers greatly reduced the soil microbial biomass and negatively impacted the soil microbial community, leading to a decrease in fungi and Gram-negative bacteria. The ratio of cyclopropyl PLFA precursors to cyclopropyl PLFAs, as a “stress factor”, indicated the most stressful bacterial environment was that found in the fertilized, non-irrigated plowed soil.
Unveiling the Role of IGF-I in Fertility: Effect of Long-Acting Bovine Somatotropin (bST) on Terminal Follicular Development and Fertility during an Annual Reproductive Cycle in Sheep
Authors: Cox José Francisco , Carrasco Albert , Navarrete Felipe , Allende Rodrigo , Saravia Fernando , Dorado Jesús
Published: April 2024

The study aimed to assess the effect of long-acting bST treatment, in a dose that only increases IGF-I plasma concentrations, on ovarian and fertility markers of estrous synchronized ewes that were fed to keep their bodyweight. Three experiments were designed to evaluate this effect: in Experiment 1, 18 ewes were distributed in groups (bST 0, 30, 50 mg) to measure plasma IGF-I and insulin for 15 days; in Experiment 2, 92 ewes (5 replicates) in two groups (0 and 30 mg bST) were synchronized using a 6-day progesterone protocol during the breeding season to assess the effect of bST on follicular and luteal performances, estrous and ovulation, and fertility after mating. In Experiment 3, 50 ewes (3 replicates) were used to repeat the study before but during anestrus. Results indicate that 50 mg bST increased IGF-I and insulin plasma concentrations, but 30 mg bST only increased IGF-I concentrations; and that only during the breeding season did 30 mg bST increase the number of lambs born and the reproductive success of ovulatory-sized follicles compared to controls. This occurred without it affecting any other reproductive marker. In conclusion, 30 mg bST treatment may improve oocyte competence for fertility during the breeding season.
Comparison of Fatty Acid Profile in Egg Yolk from Late-Age Hens Housed in Enriched Cages and in a Free Range System
Authors: Peng Meng , Tavaniello Siria , Banaszak Mirosław , Wlaźlak Sebastian , Palazzo Marisa , Grassi Giulia , Maiorano Giuseppe
Published: April 2024

In recent years, the free-range system for laying hens has increased, driven by societal sensitivity to animal welfare. This study aimed to comparatively analyze the total lipid, cholesterol, and fatty acid composition of egg yolks of late-age laying hens reared in enriched cages (C) and the free-range system (FR). Eggs were collected from Lohmann Brown Classic hens at the 68th, 70th, 72nd, and 74th week of age. The concentrations of total lipids and cholesterol were not affected (p > 0.05) by either factor. Egg yolk from the FR group showed lower (p < 0.01) monounsaturated fatty acids and higher (p < 0.01) polyunsaturated fatty acid (PUFA) compared with that of the C group. From a nutritional point of view, the PUFA n-6/n-3 and the PUFA/SFA ratios of egg yolk from the FR group were favorably lower and higher (p < 0.01) compared with the C one. Conversely, hen age did not affect (p > 0.05) the fatty acid composition of yolks. Interactions between factors were found for total n-3 and n-6 PUFA and the n-6/n-3 ratio (p < 0.01), as well as the thrombogenic index (p < 0.05). In conclusion, the results confirmed that the free-range system may improve the nutritional yolk fatty acid profile and its positive impact on human health.
Interleukin-6 as a Milk Marker of Clinical and Subclinical Intramammary Infections (IMI) in Cows Caused by Streptococcus spp.
Authors: Bochniarz Mariola , Ziomek Monika , Szczubiał Marek , Dąbrowski Roman , Wochnik Marco , Kurek Łukasz , Kosior-Korzecka Urszula , Nowakiewicz Aneta
Published: April 2024

The aim of the study was to evaluate the concentrations of Interleukin-6 (IL-6) in milk and serum of healthy cows (HE) and cows with mastitis caused by Streptococcus spp. The blood and milk samples were obtained from Holstein-Friesian cows (Lublin region, Poland). A total of 43 milk and serum samples from 28 cows with mastitis and 15 healthy cows were selected for study. IL-6 levels in milk from HE cows ranged from 6.09–80.24 pg/mL (median 26.6 pg/mL) and were significantly lower than in milk from both cows with clinical and subclinical mastitis (487.09 pg/mL vs. 26.6 pg/mL in CM, p < 0.001; and 165.31 pg/mL vs. 26.6 pg/mL in SCM, p < 0.001). The IL-6 concentration in the serum of HE was not significantly different from the serum IL-6 of the entire group of mastitis cows, regardless of whether the inflammation proceeded in a clinical or subclinical form (44.37 pg/mL vs. 78.09 pg/mL; 128.29 pg/mL vs. 78.09 pg/mL, respectively). The present study indicates that cows with mastitis caused by Streptococcus spp. develop a local immune response in the mammary gland in response to the pathogen. Monitoring of IL-6 levels in milk can allow early detection of mastitis, which is especially important in cases of subclinical inflammation.
Dosing Regimen for Cefotaxime Should Be Adapted to the Stage of Renal Dysfunction in Critically Ill Adult Patients—A Retrospective Study
Authors: Dillies Théo , Perinel-Ragey Sophie , Correia Patricia , Morel Jérôme , Thiery Guillaume , Launay Manon
Published: April 2024

Cefotaxime administration is recommended in doses of 3–12 g/day in adults with a Glomerular Filtration Rate (GFR) > 5 mL/min. This study aimed to assess the impact of renal function and obesity on cefotaxime concentrations in intensive care unit (ICU) patients. A retrospective cohort study was conducted on consecutive ICU patients receiving continuous cefotaxime infusion between 2020 and 2022 [IRBN992021/CHUSTE]. Doses were not constant; consequently, a concentration-to-dose ratio (C/D) was considered. Statistical analysis was performed to assess the relationship between cefotaxime concentrations, renal function, and obesity. A total of 70 patients, median age 61 years, were included, with no significant difference in cefotaxime concentrations between obese and non-obese patients. However, concentrations varied significantly by GFR, with underdosing prevalent in patients with normal to increased renal function and overdosing in those with severely impaired renal function. Adjustment of cefotaxime dosing according to GFR was associated with improved target attainment. Cefotaxime dosing in critically ill patients should consider renal function, with higher initial doses required in patients with normal to increased GFR and lower doses in those with severely impaired renal function. Therapeutic drug monitoring may aid in optimising dosing regimens. Prospective studies are warranted to validate these findings and inform clinical practice.
Dalbavancin for Acute Bacterial Skin and Skin Structure Infections in Pediatrics: Insights from Continuation Therapy Experience
Authors: Scarano Sara Maria , Bruzzese Eugenia , Poeta Marco , Del Bene Margherita , Guarino Alfredo , Lo Vecchio Andrea
Published: April 2024

Acute Bacterial Skin and Skin Structure Infections (ABSSSI) are marked by substantial morbidity, frequent need for hospitalization, and long courses of intravenous antibiotic therapy. Herein, we report four cases of pediatric patients admitted for ABSSSI and managed with a combination antibiotic regimen incorporating dalbavancin: a second-generation lipoglycopeptide active against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus. In our experience, particularly in a setting with a high methicillin-resistance rate, dalbavancin demonstrated safety and efficacy, simplifying ABSSSI management in childhood. Its prolonged half-life enables a single-dose administration regimen, offering potential solutions to numerous challenges encountered in pediatric care, such as extended hospital stays, difficulties in securing and maintaining vascular access, lack of pediatric-specific drug indications, and limited availability of suitable oral formulations.
Protective Effect of Curcumin on D-Galactose-Induced Senescence and Oxidative Stress in LLC-PK1 and HK-2 Cells
Authors: García-Trejo Semiramis Stephania , Gómez-Sierra Tania , Eugenio-Pérez Dianelena , Medina-Campos Omar Noel , Pedraza-Chaverri José
Published: April 2024

D-galactose has been widely used as an inducer of cellular senescence and pathophysiological processes related to aging because it induces oxidative stress. On the other hand, the consumption of antioxidants such as curcumin can be an effective strategy to prevent phenotypes related to the enhanced production of reactive oxygen species (ROS), such as aging and senescence. This study aimed to evaluate the potential protective effect of curcumin on senescence and oxidative stress and endoplasmic reticulum stress induced by D-galactose treatment in Lilly Laboratories Culture-Porcine Kidney 1 (LLC-PK1) and human kidney 2 (HK-2) proximal tubule cell lines from pig and human, respectively. For senescence induction, cells were treated with 300 mM D-galactose for 120 h and, to evaluate the protective effect of the antioxidant, cells were treated with 5 µM curcumin for 24 h and subsequently treated with curcumin + D-galactose for 120 h. In LLC-PK1 cells, curcumin treatment decreased by 20% the number of cells positive for senescence-associated (SA)-β-D-galactosidase staining and by 25% the expression of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and increased by 40% lamin B1 expression. In HK-2 cells, curcumin treatment increased by 60% the expression of proliferating cell nuclear antigen (PCNA, 50% Klotho levels, and 175% catalase activity. In both cell lines, this antioxidant decreased the production of ROS (20% decrease for LLC-PK1 and 10 to 20% for HK-2). These data suggest that curcumin treatment has a moderate protective effect on D-galactose-induced senescence in LLC-PK1 and HK-2 cells.
Contributions of Alveolar Bone Density and Habitual Chewing Side to the Unilateral Failure of Orthodontic Mini-Screws: A Cross-Sectional Study
Authors: Okuzawa-Iwasaki Makiko , Ishida Yuji , Ishizaki-Terauchi Aiko , Shimizu-Tomoda Chiyo , Aida Jun , Ono Takashi
Published: April 2024

This study aimed to analyze the relationship between bone density, habitual chewing side (HCS), and mini-screw stability to investigate the intra-individual factors contributing to mini-screw failure. This retrospective study included 86 sides in 43 adults, who underwent bilateral maxillary mini-screw placement with subsequent unilateral failure of the mini-screw. Pre-treatment cone-beam computed tomography was used to measure the buccal cortical bone thickness and bone density on the failed and successful sides. Pre-treatment mandibular kinesiographic records were used to determine the HCS. Paired t-tests, one-proportion z-tests, and multivariable multilevel Poisson regression were used to examine the statistical significance. The buccal cortical bone thicknesses were 0.93 ± 0.27 mm (unsuccessful side) and 1.01 ± 0.27 mm (successful side), with no significant difference. The bone density on the unsuccessful side (1059.64 ± 202.64 mg/cm3) was significantly lower than the success side (1317.89 ± 332.23 mg/cm3). Regarding HCS, 27.9% of failures occurred on the preferred side, and 62.8% occurred on the non-preferred side. After adjusting for all factors, the non-preferred side showed a 2.22 times higher prevalence ratio for mini-screw failure than the preferred side. HCS is significantly related to mini-screw stability, while the cortical bone thickness, bone density, and site of mini-screw implantation were not correlated.
Acoustic Emission Monitoring in Prestressed Concrete: A Comparative Study of Signal Attenuation from Wire Breaks and Rebound Hammer Impulses
Authors: Käding Max , Marx Steffen
Published: April 2024

Acoustic emission monitoring (AEM) has emerged as an effective technique for detecting wire breaks resulting from, e.g., stress corrosion cracking, and its application on prestressed concrete bridges is increasing. The success of this monitoring measure depends crucially on a carefully designed sensor layout. For this, the attenuation of elastic waves within the structure’s material is ideally determined in situ through object-related measurements (ORMs) with a reproducible signal source, typically a rebound hammer. This assumes that the attenuation coefficients derived from rebound hammer tests are comparable to those from wire breaks, thus allowing their results to be directly applied to wire break detection without further adjustments. This study challenges this assumption by analysing attenuation behaviour through an extensive dataset. Employing time-domain and frequency analysis, the research generates attenuation profiles from laboratory experiments and in situ measurements across various girders and bridge structures, extracting the slope and residual standard deviation (RSD). While generally validating this approach, the findings highlight differences in attenuation behaviour from among wire break signals and rebound hammer impulses, whereby the latter potentially underestimates the relevant attenuation of wire breaks by approximately 20%. Consequently, a transfer factor is proposed to adjust ORM results obtained with the rebound hammer for wire break scenarios. It consists of a scaling factor of 1.2 to modify the average attenuation coefficient and a constant term of ±1.0 dB/m to cover a 95% confidence interval, and thus, account for sample scattering. Moreover, the anisotropic attenuation behaviour across different structures was studied, showing that transverse attenuation consistently exceeds the longitudinal, significantly influenced by structural features such as voids. In prefabricated concrete bridges with in situ-cast concrete slabs, transverse signal transmission remains unhindered across multiple elements. Finally, the results provide a valuable reference for the design of sensor layouts in bridge monitoring, particularly benefiting scenarios where direct in situ experiences are lacking.
Effects of Bone Marrow Sparing and TGF-β3 Treatment in Total Body Irradiation of C57BL/6J Mice
Authors: Hanson Ingunn , Vatne Jenny T. , Edin Nina F. J.
Published: April 2024

Introduction: Mortality from acute radiation syndrome is frequently caused by hematopoietic or gastrointestinal radiotoxicity, the latter of which currently has no effective treatment. Transforming growth factor-beta 3 (TGF-β3) may decrease the severity of radiation-induced gastrointestinal damage in mice. In addition, treatment with TGF-β3 may alleviate radiation-induced fibrosis. Objectives: The current study aimed to investigate the effect of TGF-β3 treatment on acute and late radiotoxicity in whole body irradiated mice. Methods: C57BL/6J mice were total body irradiated with 8.5 Gy X-rays with or without shielding of one hind leg to alleviate hematopoietic radiotoxicity. The effects of intravenous TGF-β3 treatment were investigated. Body weight and pain expression were monitored. Intestine, lung, and liver tissues were preserved and analyzed. Alpha smooth muscle actin (α-SMA) expression in MRC-5 cells after 3.5 Gy X-irradiation combined with TGF-β3 treatment was analyzed using flow cytometry. Results: All total body irradiated animals died within ten days after irradiation. Ninety-three percent of femur-shielded mice survived until sampling or termination. No effect of TGF-β3 treatment was observed in either group. No increase in collagen content was detected in the lungs or liver from irradiated mice regardless of TGF-β3 treatment. In vitro, α-SMA expression increased synergistically after irradiation and TGF-β3 treatment. Conclusions: Shielding of the femur during total body irradiation decreased acute gastrointestinal radiation toxicity and increased survival. TGF-β3 treatment did not impact symptoms or survival. TGF-β3 treatment and irradiation increased α-SMA expression in MRC-5 cells synergistically.
Comparing Hierarchical Approaches to Enhance Supervised Emotive Text Classification
Authors: Williams Lowri , Anthi Eirini , Burnap Pete
Published: April 2024

The performance of emotive text classification using affective hierarchical schemes (e.g., WordNet-Affect) is often evaluated using the same traditional measures used to evaluate the performance of when a finite set of isolated classes are used. However, applying such measures means the full characteristics and structure of the emotive hierarchical scheme are not considered. Thus, the overall performance of emotive text classification using emotion hierarchical schemes is often inaccurately reported and may lead to ineffective information retrieval and decision making. This paper provides a comparative investigation into how methods used in hierarchical classification problems in other domains, which extend traditional evaluation metrics to consider the characteristics of the hierarchical classification scheme, can be applied and subsequently improve the classification of emotive texts. This study investigates the classification performance of three widely used classifiers, Naive Bayes, J48 Decision Tree, and SVM, following the application of the aforementioned methods. The results demonstrated that all the methods improved the emotion classification. However, the most notable improvement was recorded when a depth-based method was applied to both the testing and validation data, where the precision, recall, and F1-score were significantly improved by around 70 percentage points for each classifier.
Brewing Mainly from Stale Bread: A Pale Ale Case Study
Authors: Coelho Pedro , Prista Catarina , Sousa Isabel
Published: April 2024

Contemporary environmental concerns have led to the prioritization of sustainable production and material circularity, no matter what the industrial field of activity. Bread waste is a major element of overall food waste since, worldwide, bread remains a widespread staple food. A considerable proportion of bread consumption is of fresh, baked bread, consumed daily, generating substantial amounts of stale bread. Therefore, efforts to reintroduce this waste into the food value chain can make a significant contribution to reaching zero food waste, which is a major target in European countries. Possible ways to produce new raw materials through starch enzymatic hydrolysis include brewing, which is an activity in which incorporating stale bread is of great interest. Mashing parameters in brewing processing are the main focus of this study, primarily the time and temperature required to acquire optimal enzymatic activity for starch-efficient hydrolysis. Extending the mashing time to 290 min, within a temperature range of 45–75 °C, allowed us to replace 50% of the required malt with stale bread, thus obtaining a successful pale ale beer. The incorporation of stale bread in a 50:50 ratio did not affect the overall character of the beer, although the alcohol levels stood around 2% below a standard beer’s average level. Depending on the brewer’s final goal, this lighter kind of beer may be well-aligned with new consumer trends supporting more sustainable and lower-alcohol beverages.
New Insights into Hsp90 Structural Plasticity Revealed by cryoEM
Authors: Minari Karine , Balasco Serrão Vitor Hugo , Borges Júlio César
Published: April 2024

Heat Shock Protein 90 (Hsp90) acts as a crucial molecular chaperone, playing an essential role in activating numerous signaling proteins. The intricate mechanism of Hsp90 involving ATPase-coupled conformational changes and interactions with cochaperone proteins has been elucidated through biochemical and structural analyses, revealing its activation mechanism and its diverse set of “client” proteins. Despite recent advancements, certain aspects of Hsp90’s ATPase-coupled mechanism remain contentious, and the specific nature of the alterations induced by Hsp90 in client proteins remains largely undiscovered. In this review, we explore the current understanding of Hsp90’s structure and function, drawing insights from single-particle cryoEM studies. Structural studies on Hsp90 using cryoEM have provided valuable insights into the structural dynamics and interactions of this molecular chaperone. CryoEM structures have been instrumental in understanding the ATPase-coupled conformational changes that Hsp90 undergoes during its chaperone cycle. We also highlight recent progress in elucidating the structure of the ATP-bound state of the complete dimeric chaperone. Furthermore, we delve into the roles played by the multitude of cochaperones that collaborate with Hsp90, providing a glimpse into their biochemical mechanisms through the newly obtained cryoEM structures of Hsp90 cochaperone complexes.
Role of TFEB in Huntington’s Disease
Authors: Ojalvo-Pacheco Javier , Yakhine-Diop Sokhna M. S. , Fuentes José M. , Paredes-Barquero Marta , Niso-Santano Mireia
Published: April 2024

Huntington’s disease (HD) is an autosomal dominant neurodegenerative disease caused by an expansion of the CAG trinucleotide repeat in exon 1 of the huntingtin (HTT) gene. This expansion leads to a polyglutamine (polyQ) tract at the N-terminal end of HTT, which reduces the solubility of the protein and promotes its accumulation. Inefficient clearance of mutant HTT (mHTT) by the proteasome or autophagy–lysosomal system leads to accumulation of oligomers and toxic protein aggregates in neurons, resulting in impaired proteolytic systems, transcriptional dysregulation, impaired axonal transport, mitochondrial dysfunction and cellular energy imbalance. Growing evidence suggests that the accumulation of mHTT aggregates and autophagic and/or lysosomal dysfunction are the major pathogenic mechanisms underlying HD. In this context, enhancing autophagy may be an effective therapeutic strategy to remove protein aggregates and improve cell function. Transcription factor EB (TFEB), a master transcriptional regulator of autophagy, controls the expression of genes critical for autophagosome formation, lysosomal biogenesis, lysosomal function and autophagic flux. Consequently, the induction of TFEB activity to promote intracellular clearance may be a therapeutic strategy for HD. However, while some studies have shown that overexpression of TFEB facilitates the clearance of mHTT aggregates and ameliorates the disease phenotype, others indicate such overexpression may lead to mHTT co-aggregation and worsen disease progression. Further studies are necessary to confirm whether TFEB modulation could be an effective therapeutic strategy against mHTT-mediated toxicity in different disease models.
Composition and Function of Neutrophil Extracellular Traps
Authors: Wang Yijie , Du Chunjing , Zhang Yue , Zhu Liuluan
Published: April 2024

Neutrophil extracellular traps (NETs) are intricate fibrous structures released by neutrophils in response to specific stimuli. These structures are composed of depolymerized chromatin adorned with histones, granule proteins, and cytosolic proteins. NETs are formed via two distinct pathways known as suicidal NETosis, which involves NADPH oxidase (NOX), and vital NETosis, which is independent of NOX. Certain proteins found within NETs exhibit strong cytotoxic effects against both pathogens and nearby host cells. While NETs play a defensive role against pathogens, they can also contribute to tissue damage and worsen inflammation. Despite extensive research on the pathophysiological role of NETs, less attention has been paid to their components, which form a unique structure containing various proteins that have significant implications in a wide range of diseases. This review aims to elucidate the components of NETs and provide an overview of their impact on host defense against invasive pathogens, autoimmune diseases, and cancer.
Myocardial Ischemia–Reperfusion Injury: Unraveling Pathophysiology, Clinical Manifestations, and Emerging Prevention Strategies
Authors: Sagris Marios , Apostolos Anastasios , Theofilis Panagiotis , Ktenopoulos Nikolaos , Katsaros Odysseas , Tsalamandris Sotirios , Tsioufis Konstantinos , Toutouzas Konstantinos , Tousoulis Dimitris
Published: April 2024

Myocardial ischemia–reperfusion injury (MIRI) remains a challenge in the context of reperfusion procedures for myocardial infarction (MI). While early revascularization stands as the gold standard for mitigating myocardial injury, recent insights have illuminated the paradoxical role of reperfusion, giving rise to the phenomenon known as ischemia–reperfusion injury. This comprehensive review delves into the intricate pathophysiological pathways involved in MIRI, placing a particular focus on the pivotal role of endothelium. Beyond elucidating the molecular intricacies, we explore the diverse clinical manifestations associated with MIRI, underscoring its potential to contribute substantially to the final infarct size, up to 50%. We further navigate through current preventive approaches and highlight promising emerging strategies designed to counteract the devastating effects of the phenomenon. By synthesizing current knowledge and offering a perspective on evolving preventive interventions, this review serves as a valuable resource for clinicians and researchers engaged in the dynamic field of MIRI.
Experimental Study on the Adhesion of Abalone to Surfaces with Different Morphologies
Authors: Xi Peng , Qiao Yanqi , Cong Qian , Cui Qingliang
Published: April 2024

To date, research on abalone adhesion has primarily analyzed the organism’s adhesion to smooth surfaces, with few studies on adhesion to non-smooth surfaces. The present study examined the surface morphology of the abalone’s abdominal foot, followed by measuring the adhesive force of the abalone on a smooth force measuring plate and five force measuring plates with different surface morphologies. Next, the adhesion mechanism of the abdominal foot was analyzed. The findings indicated that the abdominal foot of the abalone features numerous stripe-shaped folds on its surface. The adhesion of the abalone to a fine frosted glass plate, a coarse frosted glass plate, and a quadrangular conical glass plate was not significantly different from that on a smooth glass plate. However, the organism’s adhesion to a small lattice pit glass plate and block pattern glass plate was significantly different. The abalone could effectively adhere to the surface of the block pattern glass plate using the elasticity of its abdominal foot during adhesion but experienced difficulty in completely adhering to the surface of the quadrangular conical glass plate. The abdominal foot used its elasticity to form an independent sucker system with each small lattice pit, significantly improving adhesion to the small lattice pit glass plate. The elasticity of the abalone’s abdominal foot created difficulty in handling slight morphological size changes in roughness, resulting in no significant differences in its adhesion to the smooth glass plate.
Changes in Cortical Activation by Transcranial Magnetic Stimulation Due to Coil Rotation Are Not Attributable to Cranial Muscle Activation
Authors: Mancuso Marco , Cruciani Alessandro , Sveva Valerio , Casula Elias , Brown Katlyn E. , Di Lazzaro Vincenzo , Rothwell John C. , Rocchi Lorenzo
Published: April 2024

Transcranial magnetic stimulation coupled with electroencephalography (TMS-EEG) allows for the study of brain dynamics in health and disease. Cranial muscle activation can decrease the interpretability of TMS-EEG signals by masking genuine EEG responses and increasing the reliance on preprocessing methods but can be at least partly prevented by coil rotation coupled with the online monitoring of signals; however, the extent to which changing coil rotation may affect TMS-EEG signals is not fully understood. Our objective was to compare TMS-EEG data obtained with an optimal coil rotation to induce motor evoked potentials (M1standard) while rotating the coil to minimize cranial muscle activation (M1emg). TMS-evoked potentials (TEPs), TMS-related spectral perturbation (TRSP), and intertrial phase clustering (ITPC) were calculated in both conditions using two different preprocessing pipelines based on independent component analysis (ICA) or signal-space projection with source-informed reconstruction (SSP-SIR). Comparisons were performed with cluster-based correction. The concordance correlation coefficient was computed to measure the similarity between M1standard and M1emg TMS-EEG signals. TEPs, TRSP, and ITPC were significantly larger in M1standard than in M1emg conditions; a lower CCC than expected was also found. These results were similar across the preprocessing pipelines. While rotating the coil may be advantageous to reduce cranial muscle activation, it may result in changes in TMS-EEG signals; therefore, this solution should be tailored to the specific experimental context.
Parafoveal Word Frequency Does Not Modulate the Effect of Foveal Load on Preview in Chinese Reading: Evidence from Eye Movements
Authors: Sun Yue , Li Sainan , Zhang Yancui , Wang Jingxin
Published: April 2024

The foveal load effect is one of the most fundamental effects in reading psychology, and also one of the most controversial issues in recent years. The foveal load effect refers to the phenomenon that the difficulty of foveal processing affects parafoveal preview. In Chinese reading, whether the foveal load effect exists, as well as whether this effect is modulated by parafoveal word frequency, remains unclear. In this study, the eye-tracking technique was used to track the eye movements of 48 subjects. Utilized the boundary paradigm with single-character words as parafoveal words, the present study manipulated foveal word frequency (high and low), parafoveal word frequency (high and low), and two types of preview (identical preview and pseudocharacter preview) to investigate these questions. The results revealed that the foveal word frequency does not influence preview, suggesting the absence of the foveal load effect when using single-character words as parafoveal words. Furthermore, parafoveal word frequency does not modulate the effect of the foveal load on the preview. This empirical evidence contributes to refining the understanding of the Chinese reading model.
Childhood Emotional Abuse, Neuroticism, Perfectionism, and Workaholism in an Italian Sample of Young Workers
Authors: Verrastro Valeria , Cuzzocrea Francesca , Calaresi Danilo , Saladino Valeria
Published: April 2024

The literature has linked childhood emotional abuse (CEA) to severe negative outcomes such as the development of several maladaptive personality traits and coping mechanisms. Nonetheless, its concurrent connection with neuroticism, perfectionism, and workaholism has not been explored. For the above reasons, the present study sought to investigate whether neuroticism and perfectionism mediate the relationship between CEA and workaholism, as well as evaluate the gender invariance of the model. The sample of the present research comprised 1176 young workers (50% women), aged 18–25, who completed validated self-report questionnaires. The findings highlighted significant positive direct and indirect paths, suggesting a complex interplay between CEA, neuroticism, perfectionism, and workaholism. Furthermore, the model exhibited no significant differences between genders, suggesting that the identified relationships are consistent across both women and men. The findings highlight the importance of identifying CEA and considering the adoption of trauma-informed approaches to manage its adverse effects, thereby potentially averting the onset of workaholism. Moreover, the results underline the necessity for customized preventive measures, aiming to mitigate traits associated with neuroticism and perfectionism as potential paths for successful therapeutic interventions.
Research Contents, Methods and Prospects of Emotional Architecture Based on a Systematic Literature Review
Authors: Ren Hongguo , Shi Minqi , Zhang Jing
Published: April 2024

In recent years, research on building perception has increasingly become a hot topic. More and more scholars have explored the user perceptions of the built environment and guided the design through the perception results. The technical method of emotional engineering can quantify people’s emotions and facilitate the exploration of users’ perceptions in the built environment. However, most of the existing research is empirical, and there is no article to review the interdisciplinary direction of architecture and emotional engineering. This review uses the PRISMA method to conduct a systematic literature review of 147 studies on building environment assessment using emotional engineering methods, and discusses the relationship between the building environment and humans, especially in terms of emotions, cognition, behavior, and physiology. Through a systematic literature review, the theoretical basis of emotional architecture is put forward. It constructs the interactive mode and theoretical framework of emotional architecture and reveals that the combination of artificial intelligence, big data and machine learning may be the new direction of emotional architecture research in the future.
Sustained Retinal Defocus Increases the Effect of Induced Myopia on the Retinal Astrocyte Template
Authors: Lin Carol R. , Toychiev Abduqodir , Ablordeppey Reynolds K. , Srinivas Miduturu , Benavente-Perez Alexandra
Published: April 2024

The aim of this article is to describe sustained myopic eye growth’s effect on astrocyte cellular distribution and its association with inner retinal layer thicknesses. Astrocyte density and distribution, retinal nerve fiber layer (RNFL), ganglion cell layer, and inner plexiform layer (IPL) thicknesses were assessed using immunochemistry and spectral-domain optical coherence tomography on seventeen common marmoset retinas (Callithrix jacchus): six induced with myopia from 2 to 6 months of age (6-month-old myopes), three induced with myopia from 2 to 12 months of age (12-month-old myopes), five age-matched 6-month-old controls, and three age-matched 12-month-old controls. Untreated marmoset eyes grew normally, and both RNFL and IPL thicknesses did not change with age, with astrocyte numbers correlating to RNFL and IPL thicknesses in both control age groups. Myopic marmosets did not follow this trend and, instead, exhibited decreased astrocyte density, increased GFAP+ spatial coverage, and thinner RNFL and IPL, all of which worsened over time. Myopic changes in astrocyte density, GFAP+ spatial coverage and inner retinal layer thicknesses suggest astrocyte template reorganization during myopia development and progression which increased over time. Whether or not these changes are constructive or destructive to the retina still remains to be assessed.
A Custom Panel for Profiling Microglia Gene Expression
Authors: Potru Phani Sankar , Vidovic Natascha , Wiemann Susanne , Russ Tamara , Trautmann Marcel , Spittau Björn
Published: April 2024

Despite being immune cells of the central nervous system (CNS), microglia contribute to CNS development, maturation, and homeostasis, and microglia dysfunction has been implicated in several neurological disorders. Recent advancements in single-cell studies have uncovered unique microglia-specific gene expression. However, there is a need for a simple yet elegant multiplexed approach to quantifying microglia gene expression. To address this, we have designed a NanoString nCounter technology-based murine microglia-specific custom codeset comprising 178 genes. We analyzed RNA extracted from ex vivo adult mouse microglia, primary mouse microglia, the BV2 microglia cell line, and mouse bone marrow monocytes using our custom panel. Our findings reveal a pattern where homeostatic genes exhibit heightened expression in adult microglia, followed by primary cells, and are absent in BV2 cells, while reactive markers are elevated in primary microglia and BV2 cells. Analysis of publicly available data sets for the genes present in the panel revealed that the panel could reliably reflect the changes in microglia gene expression in response to various factors. These findings highlight that the microglia panel used offers a swift and cost-effective means to assess microglial cells and can be used to study them in varying contexts, ranging from normal homeostasis to disease models.
Thioredoxin Domain Containing 5 (TXNDC5): Friend or Foe?
Authors: Bidooki Seyed Hesamoddin , Navarro María A. , Fernandes Susana C. M. , Osada Jesus
Published: April 2024

This review focuses on the thioredoxin domain containing 5 (TXNDC5), also known as endoplasmic reticulum protein 46 (ERp46), a member of the protein disulfide isomerase (PDI) family with a dual role in multiple diseases. TXNDC5 is highly expressed in endothelial cells, fibroblasts, pancreatic β-cells, liver cells, and hypoxic tissues, such as cancer endothelial cells and atherosclerotic plaques. TXNDC5 plays a crucial role in regulating cell proliferation, apoptosis, migration, and antioxidative stress. Its potential significance in cancer warrants further investigation, given the altered and highly adaptable metabolism of tumor cells. It has been reported that both high and low levels of TXNDC5 expression are associated with multiple diseases, such as arthritis, cancer, diabetes, brain diseases, and infections, as well as worse prognoses. TXNDC5 has been attributed to both oncogenic and tumor-suppressive features. It has been concluded that in cancer, TXNDC5 acts as a foe and responds to metabolic and cellular stress signals to promote the survival of tumor cells against apoptosis. Conversely, in normal cells, TXNDC5 acts as a friend to safeguard cells against oxidative and endoplasmic reticulum stress. Therefore, TXNDC5 could serve as a viable biomarker or even a potential pharmacological target.
Structural Evolution and Fracture Mechanism of WC-Particle-Reinforced FeCoCrNiMn High-Entropy Alloy Coatings
Authors: Wang Xinbo , Zhang Shihan , Zhao Fei , Wu Zhisheng , Xie Zhiwen
Published: April 2024

A hard-particle-reinforced high-entropy alloy (HEA) coating shows significant potential for tribological applications, but relatively little work on the fracture mechanism of the coating has been reported. In this work, the FeCoCrNiMn HEA coatings, doped with varying contents of WC, were fabricated using a plasma surfacing technique. The structure, mechanical properties, and fracture behaviors of these coatings were investigated through X-ray diffraction (XRD), scanning electron microscopy (SEM), and hardness and tensile tests. The addition of WC particles significantly altered the growth mode of the coating texture from large coarse grains to fine grains. The coating without WC doping had a hardness of 198.8 ± 15.6 HV, a yield strength of 225 MPa, a tensile strength of 478 MPa, and a strain of 53.7%. The hardness, yield strength, tensile strength, and strain were 222.3 ± 34.4 HV, 353 MPa, 704 MPa, and 42.6% for the coating with 10% WC doping and 355.6 ± 51.6 HV, 454 MPa, 627 MPa, and 9.4% for the coating with 20% WC doping. Meanwhile, the coating with 40% WC doping showed the greatest hardness of 514.9 ± 48.1 HV and had the highest yield strength of 457 MPa, but its tensile strength and strain decreased to 517 MPa and 2.7%, respectively. In this article, the detailed structural evolution, strengthening, and fracture failure mechanisms of the coatings are discussed systematically.
Machine Learning-Based Predictions of Porosity during Cold Spray Deposition of High Entropy Alloy Coatings
Authors: Sharma Deepak , Boruah Dibakor , Bakir Ali Alperen , Ameen Ahamed , Paul Shiladitya
Published: April 2024

Porosity poses a challenge to the mechanical properties of cold sprayed coatings, especially when it is open or surface-connected, limiting the coatings’ capabilities to act as a barrier. The porosity formation is dependent on the feedstock powder characteristics and the cold spray process parameters. We present a machine learning-based approach to predict porosity based on the above-mentioned factors. Nine different machine learning models based on linear regression (LR), decision trees, random forests, gradient boosting, support vector machine (SVM), and neural networks were explored. Considering the excellent properties of high entropy alloys, Cantor alloy was taken as the consumable. Our dataset, derived from the literature and experiments, identified SVM with a linear kernel and LR as the top-performing models based on the Pearson correlation coefficient (PCC) and root mean square error, where the PCC values exceeded 0.8. The SHapley Additive exPlanations method helped in identifying that the type of gas and powder are the top two factors in pore formation.