bluuebunny/crossref_metadata_2025 huggingface dataset:
Broad-Spectrum HDAC Inhibitors Promote Autophagy through FOXO Transcription Factors in Neuroblastoma
Authors: Körholz Katharina , Ridinger Johannes , Krunic Damir , Najafi Sara , Gerloff Xenia F. , Frese Karen , Meder Benjamin , Peterziel Heike , Vega-Rubin-de-Celis Silvia , Witt Olaf , Oehme Ina
Published: April 2021

Depending on context and tumor stage, deregulation of autophagy can either suppress tumorigenesis or promote chemoresistance and tumor survival. Histone deacetylases (HDACs) can modulate autophagy; however, the exact mechanisms are not fully understood. Here, we analyze the effects of the broad-spectrum HDAC inhibitors (HDACi) panobinostat and vorinostat on the transcriptional regulation of autophagy with respect to autophagy transcription factor activity (Transcription factor EB—TFEB, forkhead boxO—FOXO) and autophagic flux in neuroblastoma cells. In combination with the late-stage autophagic flux inhibitor bafilomycin A1, HDACis increase the number of autophagic vesicles, indicating an increase in autophagic flux. Both HDACi induce nuclear translocation of the transcription factors FOXO1 and FOXO3a, but not TFEB and promote the expression of pro-autophagic FOXO1/3a target genes. Moreover, FOXO1/3a knockdown experiments impaired HDACi treatment mediated expression of autophagy related genes. Combination of panobinostat with the lysosomal inhibitor chloroquine, which blocks autophagic flux, enhances neuroblastoma cell death in culture and hampers tumor growth in vivo in a neuroblastoma zebrafish xenograft model. In conclusion, our results indicate that pan-HDACi treatment induces autophagy in neuroblastoma at a transcriptional level. Combining HDACis with autophagy modulating drugs suppresses tumor growth of high-risk neuroblastoma cells. These experimental data provide novel insights for optimization of treatment strategies in neuroblastoma.
Proteomic Investigation of Glyceraldehyde-Derived Intracellular AGEs and Their Potential Influence on Pancreatic Ductal Cells
Authors: Senavirathna Lakmini , Ma Cheng , Chen Ru , Pan Sheng
Published: April 2021

Glyceraldehyde-derived advanced glycation end products (AGEs) play an important role in the pathogenesis of many diseases including cancer. Accumulation of intracellular AGEs could stimulate cancer induction and facilitate cancer progression. We evaluated the toxic effect of glyceraldehyde-derived intracellular AGEs on normal and malignant pancreatic ductal cells by assessing the cell viability, toxicity, and oxidative stress, followed by proteomic analysis. Our functional studies showed that pancreatic cancer cells (PANC-1 and MIA PaCa-2) were more resistant to glyceraldehyde treatment compared to normal pancreatic ductal epithelial cells (HPDE), while cytotoxicity effects were observed in all cell types. Furthermore, using 13C isotopic labeled glyceraldehyde, the proteomic data revealed a dose-dependent increment of the number of glycation adducts in both these cell types. HPDE cells showed a higher number of intracellular AGEs compared to cancer cells. At a molecular level, the glycations in the lysine residues of proteins showed a concurrent increase with the concentration of the glyceraldehyde treatment, while the arginine glycations appeared to be less affected by the glyceraldehyde doses. Further pathway analysis of these glycated proteins suggested that the glycated proteins participate in important biological processes that are major hallmarks of cancer initiation and progression, including metabolic processes, immune response, oxidative stress, apoptosis, and S100 protein binding.
Tumour Hypoxia-Mediated Immunosuppression: Mechanisms and Therapeutic Approaches to Improve Cancer Immunotherapy
Authors: Fu Zhe , Mowday Alexandra M. , Smaill Jeff B. , Hermans Ian F. , Patterson Adam V.
Published: April 2021

The magnitude of the host immune response can be regulated by either stimulatory or inhibitory immune checkpoint molecules. Receptor-ligand binding between inhibitory molecules is often exploited by tumours to suppress anti-tumour immune responses. Immune checkpoint inhibitors that block these inhibitory interactions can relieve T-cells from negative regulation, and have yielded remarkable activity in the clinic. Despite this success, clinical data reveal that durable responses are limited to a minority of patients and malignancies, indicating the presence of underlying resistance mechanisms. Accumulating evidence suggests that tumour hypoxia, a pervasive feature of many solid cancers, is a critical phenomenon involved in suppressing the anti-tumour immune response generated by checkpoint inhibitors. In this review, we discuss the mechanisms associated with hypoxia-mediate immunosuppression and focus on modulating tumour hypoxia as an approach to improve immunotherapy responsiveness.
Transcriptome Analysis of Hypoxic Lymphatic Endothelial Cells Indicates Their Potential to Contribute to Extracellular Matrix Rearrangement
Authors: Becker Jürgen , Schwoch Sonja , Zelent Christina , Sitte Maren , Salinas Gabriela , Wilting Jörg
Published: April 2021

Lymphedema (LE) affects millions of people worldwide. It is a chronic progressive disease with massive development of fibrosclerosis when untreated. There is no pharmacological treatment of lymphedema. The disease is associated with swelling of the interstitium of the affected organ, mostly arm or leg, impressive development of adipose tissue, fibrosis and sclerosis with accumulation of huge amounts of collagen, and Papillomatosis cutis. Malnutrition and reduced oxygenation of the affected tissues is a hallmark of lymphedema. Here, we investigated if the hypoxia of lymphatic endothelial cells (LECs) might contribute to fibrosis. We applied RNASeq and qPCR to study the concordant changes of the exome of three human foreskin-derived LEC isolates after 4 days of hypoxia (1% O2) vs. normoxia (21% O2). Of the approximately 16,000 genes expressed in LECs, 162 (1%) were up- or down-regulated by hypoxia. Of these, 21 genes have important functions in the production or modification of the extracellular matrix (ECM). In addition to the down-regulation of elastin, we found up-regulation of druggable enzymes and regulators such as the long non-coding RNA H19, inter-alpha-trypsin inhibitor heavy chain family member 5 (ITIH5), lysyl-oxidase (LOX), prolyl 4-hydroxylase subunit alpha 1 (P4HA1), procollagen-lysine 2-oxoglutarate 5-dioxygenase 2 (PLOD2), and others that are discussed in the paper. Initial lymphatics do not produce a continuous basement membrane; however, our study shows that hypoxic LECs have an unexpectedly high ability to alter the ECM.
Glassy Carbon Electrode Modified with C/Au Nanostructured Materials for Simultaneous Determination of Hydroquinone and Catechol in Water Matrices
Authors: Piña Samuel , Candia-Onfray Christian , Hassan Natalia , Jara-Ulloa Paola , Contreras David , Salazar Ricardo
Published: April 2021

The simultaneous determination of hydroquinone and catechol was conducted in aqueous and real samples by means of differential pulse voltammetry (DPV) using a glassy carbon electrode modified with Gold Nanoparticles (AuNP) and functionalized multiwalled carbon nanotubes by drop coating. A good response was obtained in the simultaneous determination of both isomers through standard addition to samples prepared with analytical grade water and multivariate calibration by partial least squares (PLS) in winery wastewater fortified with HQ and CT from 4.0 to 150.00 µM. A sensitivity of 0.154 µA µM−1 and 0.107 µA µM−1, and detection limits of 4.3 and 3.9 µM were found for hydroquinone and catechol, respectively. We verified the reliability of the developed method by simultaneously screening analytes in spiked tap water and industrial wastewater, achieving recoveries over 80%. In addition, this paper demonstrates the applicability of chemometric tools for the simultaneous quantification of both isomers in real matrices, obtaining prediction errors of lower than 10% in fortified wastewater.
Climate Change in the 2019 Canadian Federal Election
Authors: Boulianne Shelley , Belland Stephanie , Sleptcov Nikita , Larsson Anders Olof
Published: April 2021

In the weeks before the 2019 federal election, climate change strikes occurred in Canada and across the globe, which may have increased the salience of this policy issue. We use two data sources to examine the role of climate change in the 2019 federal election: a representative survey of 1500 Canadians and 2109 Facebook posts from the five major party leaders. After accounting for political ideology and region, we find that concern about climate change was a strong positive predictor of liberal support. We triangulate these findings by analyzing Facebook posts. We find that left-wing politicians were more likely to post about climate change and that posts about climate change received more likes, comments, and shares than other posts. This higher level of user engagement did not differ depending on which political party posted the climate change message. The combination of sources offers news insights into citizen-elite interactions and electoral outcomes. Climate change was important in the election, whether this importance was measured through survey data or user engagement with leaders’ climate change posts.
Experimental Comparison of Innovative Composite Sorbents for Space Heating and Domestic Hot Water Storage
Authors: Brancato Vincenza , Gordeeva Larisa G. , Caprì Angela , Grekova Alexandra D. , Frazzica Andrea
Published: April 2021

In this study, the development and comparative characterization of different composite sorbents for thermal energy storage applications is reported. Two different applications were targeted, namely, low-temperature space heating (SH) and domestic hot water (DHW) provision. From a literature analysis, the most promising hygroscopic salts were selected for these conditions, being LiCl for SH and LiBr for DHW. Furthermore, two mesoporous silica gel matrixes and a macroporous vermiculite were acquired to prepare the composites. A complete characterization was performed by investigating the porous structure of the composites before and after impregnation, through N2 physisorption, as well as checking the phase composition of the composites at different temperatures through X-ray powder diffraction (XRD) analysis. Furthermore, sorption equilibrium curves were measured in water vapor atmosphere to evaluate the adsorption capacity of the samples and a detailed calorimetric analysis was carried out to evaluate the reaction evolution under real operating conditions as well as the sorption heat of each sample. The results demonstrated a slower reaction kinetic in the vermiculite-based composites, due to the larger size of salt grains embedded in the pores, while promising volumetric storage densities of 0.7 GJ/m3 and 0.4 GJ/m3 in silica gel-based composites were achieved for SH and DHW applications, respectively.
Effect of High Pressure on the Solidification of Al–Ni Alloy
Authors: Wang Xiao-Hong , Dong Duo , Yang Xiao-Hong
Published: April 2021

The effect of high pressure on the microstructure of hypo-peritectic Al–38wt.%Ni alloy was studied. The results show that Al3Ni and Al3Ni2 phases coexist at ambient pressure. However, it becomes a typical hyper-eutectic microstructure when synthesized at 2 GPa and 4 GPa. Meanwhile, the interface temperature of Al3Ni and Al3Ni2 phases was calculated with the combination of the BCT dendrite growth model, which is suitable for the Al3Ni2 phase. According to the highest interface temperature principle, the result shows that the Al3Ni phase dominates over 1–5 GPa. Finally, the Debye temperature and potential energy of the hypo-peritectic Al–38wt.%Ni alloy under different pressures were researched. Based on the low temperature specific heat-capacity curve. The Debye temperatures at ambient pressure, 2 GPa, and 4 GPa are 504.4 K, 508.71 K and 515.36 K, respectively, and the potential energy in the lowest point decreases with the increase of pressure.
HER2 Positive and HER2 Negative Classical Type Invasive Lobular Carcinomas: Comparison of Clinicopathologic Features
Authors: He Lin , Araj Ellen , Peng Yan
Published: April 2021

Human epidermal growth factor receptor 2 (HER2) positive (+) classical type invasive lobular carcinoma (cILC) of the breast is extremely rare and its clinicopathologic features have not been well characterized. We compared features of HER2(+) and HER2 negative (−) cILCs. A total of 29 cases were identified from the clinical database at our institution from 2011-2019; 9 were HER2(+) cILC tumors and 20 were HER2(−) cILC tumors. The results reveal that HER2(+) cILC group had significantly increased Ki-67 expression and reduced estrogen receptor (ER) expression compared to HER2(−) cILC group (both p < 0.05). In addition, HER2(+) cILCs tended to be diagnosed at a younger age and more common in the left breast, and appeared to have a higher frequency of nodal or distant metastases. These clinicopathologic features suggest HER2(+) cILC tumors may have more aggressive behavior than their HER2(−) counterpart although both groups of tumors showed similar morphologic features. Future directions of the study: (1) To conduct a multi-institutional study with a larger case series of HER2(+) cILC to further characterize its clinicopathologic features; (2) to compare molecular profiles by next generation sequencing (NGS) assay between HER2(+) cILC and HER2(−) cILC cases to better understand tumor biology of this rare subset of HER2(+) breast cancer; and (3) to compare molecular characteristics of HER2(+) cILC and HER2(+) high grade breast cancer in conjunction with status of tumor response to anti-HER2 therapy to provide insight to management of this special type of low grade breast cancer to avoid unnecessary treatment and related toxicity
Effect of Potato By-Product on Production Responses of Dairy Cows and Total Mixed Ration Stability
Authors: Franco Marcia , Stefański Tomasz , Jalava Taina , Lehto Marja , Kahala Minna , Järvenpää Eila , Mäntysaari Päivi , Rinne Marketta
Published: April 2021

Vegetable by-products have great potential for use as animal feeds and thus could improve the sustainability of the food system. The objective was to evaluate the milk production potential of potato by-product (PBP) replacing cereal grains in grass silage-based total mixed ration (TMR). Additionally, a laboratory scale experiment was conducted to assess the effect of PBP and chemical preservatives on the aerobic stability of TMRs. A change-over dairy cow feeding experiment was conducted to evaluate the inclusion of 135 g/kg (on a dry matter (DM) basis) of PBP in TMR. Additionally, the aerobic stability of TMR was evaluated by preparing TMRs with increasing levels of PBP and treated with different doses of formic and propionic acid or salt-based preservatives. The inclusion of PBP in dairy cow diets decreased feed intake, but organic matter digestibility was greater in the PBP diet, resulting in only slightly decreased production (milk protein 1435 vs. 1363 g/day, p < 0.05; milk lactose 1716 vs. 1606 g/day, p < 0.05). The aerobic stability of TMR was negatively affected by the inclusion of PBP, but it could to a limited extent be prolonged using chemical preservatives. The inclusion of PBP decreased feed intake and milk production to some extent when included in the diet of high-producing dairy cows. The utilization of PBP may, however, be justified from a sustainability point of view, as it provides a way to circulate the nutrients of a non-human edible feed material back into the food chain.
Estimated Effects of Web-Based Course from Adult Vocational Students’ Perspective—A PFA Course in Scandinavian Intellectual Disability Practice
Authors: Olsson Sylvia , Gustafsson Christine
Published: April 2021

In Scandinavia, the field of intellectual disability (ID) practice lacks specific course, professional training, theoretical foundation, guidelines, and practice consensus. However, Pedagogiskt Förhållnings-och Arbetsätt (PFA model) has the potential to offer a pedagogical attitude and a way of working within ID practice. PFA was developed in the context of Swedish ID practice and has attracted national and European interest. Most staff working in ID practice in Scandinavia are educated to upper secondary school (adolescent students) level or vocational course (adult students) level. In the present pilot study, we examined the effects of providing a web-based PFA course as a complement to the Swedish national curriculum. The aim of this pilot study was to assess the effects of self-assessed knowledge and learning using a web-based PFA course intervention on an adult vocational course. The study involved a quantitative intervention approach involving two equivalent groups in an experimental design: an experimental group (n = 12) and a comparison group (n = 46). These groups were administered a pre-test and a post-test questionnaire that estimated their knowledge of the course through self-reporting tests. The findings provide empirical evidence of the informed and sustained use of knowledge building through complementary web-based PFA courses.
Arbitrary-Order Finite-Time Corrections for the Kramers–Moyal Operator
Authors: Rydin Gorjão Leonardo , Witthaut Dirk , Lehnertz Klaus , Lind Pedro G.
Published: April 2021

With the aim of improving the reconstruction of stochastic evolution equations from empirical time-series data, we derive a full representation of the generator of the Kramers–Moyal operator via a power-series expansion of the exponential operator. This expansion is necessary for deriving the different terms in a stochastic differential equation. With the full representation of this operator, we are able to separate finite-time corrections of the power-series expansion of arbitrary order into terms with and without derivatives of the Kramers–Moyal coefficients. We arrive at a closed-form solution expressed through conditional moments, which can be extracted directly from time-series data with a finite sampling intervals. We provide all finite-time correction terms for parametric and non-parametric estimation of the Kramers–Moyal coefficients for discontinuous processes which can be easily implemented—employing Bell polynomials—in time-series analyses of stochastic processes. With exemplary cases of insufficiently sampled diffusion and jump-diffusion processes, we demonstrate the advantages of our arbitrary-order finite-time corrections and their impact in distinguishing diffusion and jump-diffusion processes strictly from time-series data.
A Unified Formulation of k-Means, Fuzzy c-Means and Gaussian Mixture Model by the Kolmogorov–Nagumo Average
Authors: Komori Osamu , Eguchi Shinto
Published: April 2021

Clustering is a major unsupervised learning algorithm and is widely applied in data mining and statistical data analyses. Typical examples include k-means, fuzzy c-means, and Gaussian mixture models, which are categorized into hard, soft, and model-based clusterings, respectively. We propose a new clustering, called Pareto clustering, based on the Kolmogorov–Nagumo average, which is defined by a survival function of the Pareto distribution. The proposed algorithm incorporates all the aforementioned clusterings plus maximum-entropy clustering. We introduce a probabilistic framework for the proposed method, in which the underlying distribution to give consistency is discussed. We build the minorize-maximization algorithm to estimate the parameters in Pareto clustering. We compare the performance with existing methods in simulation studies and in benchmark dataset analyses to demonstrate its highly practical utilities.
Quantum Randomness is Chimeric
Authors: Svozil Karl
Published: April 2021

If quantum mechanics is taken for granted, the randomness derived from it may be vacuous or even delusional, yet sufficient for many practical purposes. “Random” quantum events are intimately related to the emergence of both space-time as well as the identification of physical properties through which so-called objects are aggregated. We also present a brief review of the metaphysics of indeterminism.
Analyzing the Coevolution of Mobile Application Diffusion and Social Network: A Multi-Agent Model
Authors: Zhang Zhenyu , Zhang Huirong , Zhou Lixin , Li Yanfeng
Published: April 2021

The successful diffusion of mobile applications in user groups can establish a good image for enterprises, gain a good reputation, fight for market share, and create commercial profits. Thus, it is of great significance for the successful diffusion of mobile applications to study mobile application diffusion and social network coevolution. Firstly, combined with a social network’s dynamic change characteristics in real life, a mobile application users’ social network evolution mechanism was designed. Then, a multi-agent model of the coevolution of a social network and mobile application innovation diffusion was constructed. Finally, the impact of mobile applications’ value perception revenue, use cost, marketing promotion investment, and the number of seed users on the coevolution of social network and mobile application diffusion were analyzed. The results show that factors such as the network structure, the perceived value income, the cost of use, the marketing promotion investment, and the number of seed users have an important impact on mobile application diffusion.
Threshold-Based Hierarchical Clustering for Person Re-Identification
Authors: Hu Minhui , Zeng Kaiwei , Wang Yaohua , Guo Yang
Published: April 2021

Unsupervised domain adaptation is a challenging task in person re-identification (re-ID). Recently, cluster-based methods achieve good performance; clustering and training are two important phases in these methods. For clustering, one major issue of existing methods is that they do not fully exploit the information in outliers by either discarding outliers in clusters or simply merging outliers. For training, existing methods only use source features for pretraining and target features for fine-tuning and do not make full use of all valuable information in source datasets and target datasets. To solve these problems, we propose a Threshold-based Hierarchical clustering method with Contrastive loss (THC). There are two features of THC: (1) it regards outliers as single-sample clusters to participate in training. It well preserves the information in outliers without setting cluster number and combines advantages of existing clustering methods; (2) it uses contrastive loss to make full use of all valuable information, including source-class centroids, target-cluster centroids and single-sample clusters, thus achieving better performance. We conduct extensive experiments on Market-1501, DukeMTMC-reID and MSMT17. Results show our method achieves state of the art.
Two Excited State Collaboration of Heteroleptic Ir(III)-Coumarin Complexes for H2 Evolution Dye-Sensitized Photocatalysts
Authors: Kobayashi Atsushi , Muramatsu Eiichirou , Yoshida Masaki , Kato Masako
Published: April 2021

Interfacial electron injection from a photoexcited surface-immobilized dye to a semiconductor substrate is a key reaction for dye-sensitized photocatalysts. We previously reported that the molecular orientation of heteroleptic Ir(III) photosensitizer on the TiO2 nanoparticle surface was important for efficient interfacial electron injection. In this work, to overcome the weak light absorption ability of heteroleptic Ir(III) photosensitizer and to improve the photoinduced charge-separation efficiency at the dye–semiconductor interface, we synthesized two heteroleptic Ir(III) complexes with different coumarin dyes, [Ir(C6)2(H4CPbpy)]Cl and [Ir(C30)2(H4CPbpy)]Cl [Ir-CX; X = 6 or 30; HC6 = 3-(2-enzothiazolyl)-7-(diethylamino)coumarin, HC30 = 3-(2-N-methylbenzimidazolyl)-7-N,N-diethylaminocoumarin, H4CPbpy = 4,4′-bis(methylphosphonic acid)-2,2′-bipyridine], as the cyclometalated ligands and immobilized them on the surface of Pt-cocatalyst-loaded TiO2 nanoparticles. Ultraviolet-visible absorption and emission spectroscopy revealed that the singlet ligand-centered (1LC) absorption and triplet 3LC emission bands of Ir-C30 occurred at shorter wavelengths than those of Ir-C6, while time-dependent density-functional-theory data suggested that the ligand-to-ligand charge transfer (LLCT) excited states of the two complexes were comparable. The photocatalytic H2 evolution activity of the Ir-C6-sensitized Pt-TiO2 nanoparticles (Ir-C6@Pt-TiO2) under visible light irradiation (λ > 420 nm) was higher than that of Ir-C30@Pt-TiO2. In contrast, their activities were comparable under irradiation with monochromatic light (λ = 450 ± 10 nm), which is absorbed comparably by both Ir-CX complexes. These results suggest that the internal conversion from the higher-lying LC state to the LLCT state effectively occurs in both Ir-CX complexes to trigger electron injection to TiO2.
Thermal Analysis of Heat Distribution in Busbars during Rated Current Flow in Low-Voltage Industrial Switchgear
Authors: Szulborski Michał , Łapczyński Sebastian , Kolimas Łukasz
Published: April 2021

The manuscript presents advanced coupled analysis: Maxwell 3D, Transient Thermal and Fluent CFD, at the time of a rated current occurring on the main busbars in the low-voltage switchgear. The simulations were procured in order to aid the design process of such enclosures. The analysis presented the rated current flow in the switchgear busbars, which allowed determining their temperature values. The main assumption of the simulation was measurements of temperature rise during rated current conditions. Simulating such conditions is a valuable asset in order to design better solutions for energy distribution gear. The simulation model was a precise representation of the actual prototype of the switchgear. Simulations results were validated by experimental research. The heat dissipation in busbars and switchgear housing through air convection was presented. The temperature distribution for the insulators in the rail bridge made of fireproof material was considered: halogen-free polyester. The results obtained during the simulation allowed for a detailed analysis of switchgear design and proper conclusions in practical and theoretical aspects. That helped in introducing structural changes in the prepared prototype of the switchgear at the design and construction stages. Deep analysis of the simulation results allowed for the development concerning the final prototype of the switchgear, which could be subjected to the full type tests. Additionally, short-circuit current simulations were procured and presented.
Techno-Economic Analysis of Waste Heat Utilization in Data Centers: Application of Absorption Chiller Systems
Authors: Amiri Leyla , Madadian Edris , Bahrani Navid , Ghoreishi-Madiseh Seyed Ali
Published: April 2021

Modern data centers are playing a pivotal role in the global economic situation. Unlike high-quality source of waste heat, it is challenging to recover the decentralized and low-quality waste heat sourced from data centers due to numerous technological and economic hurdles. As such, it is of the utmost importance to explore possible pathways to maximize the energy efficiency of the data centers and to utilize their heat recovery. Absorption chiller systems are a promising technology for the recovery of waste heat at ultra-low temperatures. In fact, the low temperature heat discharged from data centers cannot be retrieved with conventional heat recovery systems. Therefore, the present study investigated feasibility of waste heat recovery from data centers using an absorption chiller system, with the ultimate goal of electrical energy production. To fulfill this objective, a techno-economic assessment of heat recovery using absorption chiller (AC) technique for the data centers with power consumption range of 4.5 to 13.5 MW is performed. The proposed AC system enables saving electricity for the value of 4,340,000 kWh/year and 13,025,000 kWh/year leading to an annual reduction of 3068 and 9208 tons CO2 equivalent of greenhouse gas (GHG) emissions, respectively. The results of this study suggest an optimum change in the design of the data center while reducing the payback period for the investors.
Use of 3D-FEM Tools to Improve Loss Allocation in Three-Core Armored Cables
Authors: del-Pino-López Juan Carlos , Cruz-Romero Pedro
Published: April 2021

Loss allocation through analytical expressions in three-core lead-sheathed armored cables is challenging due to the complex geometry of this type of cable, commonly employed in submarine energy transmission systems (involving twisted conductors, sheaths and armor). Most of the expressions of the IEC standard 60287 have not been properly adapted for three-core armored cables, leading to inaccurate values for the different losses, so important efforts are currently devoted to improving them. In this work, an improved ultra-shortened 3D finite element model (FEM) is employed for developing an in-depth analysis of the electromagnetic interactions that take place in 6 real cables, being especially focused on those aspects that are not considered in the IEC standard. As a result, important conclusions are derived regarding the losses in conductors and sheaths, which introduce different corrections for improving the accuracy of the IEC expressions. The new formulation is then employed to propose a simplified experimental armor loss allocation procedure. This is virtually applied through the FEM tool to more than 700 cable configurations, showing a remarkable improvement in the loss allocation over the IEC standard and previous experimental procedures.
Low Frequency Damping Control for Power Electronics-Based AC Grid Using Inverters with Built-In PSS
Authors: Yang Ming , Cao Wu , Lin Tingjun , Zhao Jianfeng , Li Wei
Published: April 2021

Low frequency oscillations are the most easily occurring dynamic stability problem in the power system. With the increasing capacity of power electronic equipment, the coupling coordination of a synchronous generator and inverter in a low frequency range is worth to be studied further. This paper analyzes the mechanism of the interaction between a normal active/reactive power control grid-connected inverters and power regulation of a synchronous generator. Based on the mechanism, the power system stabilizer built in the inverter is used to increase damping in low frequency range. The small-signal model for electromagnetic torque interaction between the grid-connected inverters and the generator is analyzed first. The small-signal model is the basis for the inverters to provide damping with specific amplitude and phase. The additional damping torque control of the inverters is realized through a built-in power system stabilizer. The fundamentals and the structure of a built-in power system stabilizer are illustrated. The built-in power system stabilizer can be realized through the active or reactive power control loop. The parameter design method is also proposed. With the proposed model and suppression method, the inverters can provide a certain damping torque to improve system stability. Finally, detailed system damping simulation results of the universal step test verify that the analysis is valid and effective.
Multi-Objective Energy Management Strategy Based on PSO Optimization for Power-Split Hybrid Electric Vehicles
Authors: Du Aimin , Chen Yaoyi , Zhang Dongxu , Han Yeyang
Published: April 2021

The hybrid electric vehicle is equipped with an internal combustion engine and motor as the driving source, which can solve the problems of short driving range and slow charging of the electric vehicle. Making an effective energy management control strategy can reasonably distribute the output power of the engine and motor, improve engine efficiency, and reduce battery damage. To reduce vehicle energy consumption and excessive battery discharge at the same time, a multi-objective energy management strategy based on a particle swarm optimization algorithm is proposed. First, a simulation platform was built based on a compound power-split vehicle model. Then, the ECMS (Equivalent Consumption Minimization Strategy) was used to realize the real-time control of the model, and the penalty function was added to modify the objective function based on the current SOC (State of Charge) to maintain the SOC balance. Finally, the key parameters of ECMS were optimized by using a particle swarm optimization algorithm, and the effectiveness of the control strategy was verified under the WLTC (Worldwide Light-Duty Test Cycle) and the NEDC (New European Driving Cycle). The results show that under the WLTC test cycle, the overall fuel consumption of the whole vehicle was 6.88 L/100 km, which was 7.7% lower than that before optimization; under the NEDC test cycle, the fuel consumption of the whole vehicle was 5.88 L/100 km, which was 9.8% lower than that before optimization.
Morphological and Physiological Responses of Pinus massoniana Seedlings to Different Light Gradients
Authors: Wang Haoyun , Wu Feng , Li Min , Zhu Xiaokun , Shi Changshuang , Ding Guijie
Published: April 2021

Light intensity is a critical factor regulating photosynthetic capacity in plants. However, the effects of varying light intensity on morphological and photoprotective mechanisms in Pinus massoniana seedlings have not been explored in depth, especially those in the first seedling growing season. We measured the growth, photosynthetic physiology, biochemistry, and chlorophyll fluorescence of P. massoniana seedlings at four light gradients: 100% relative irradiance (RI, full sunlight), 70% RI, 50% RI, and 20% RI. The seedling height at 70% RI was 9.27% higher than that at 100% RI. However, seedling height was inhibited under low light intensity; at 20% RI, all seedlings died. The decreasing light intensity inhibited ground diameter growth but increased the height-diameter ratio. The secondary needle emergence rate was 53.4% higher at 70% RI than at 100% RI but was only 2% at 50% RI. The chlorophyll and carotenoid contents increased significantly with decreasing light intensity. The increased Chl b and Car contents promoted the photoreceptor potential of the violet (400~420 nm), blue (440~480 nm), and yellow-orange (597~655 nm) regions in leaves. Among the chlorophyll fluorescence parameters, Fv/Fm, Fv′/Fm′, Y(II), qp, and ETR all reached maximum values at 70% RI but were significantly lower at 50% RI than at 100% RI. However, decreasing the light intensity caused a reduction in NPQ. The 70% RI level increased POD and SOD activity and the contents of osmotic regulation substances and slowed MDA accumulation. Seedlings at 70% RI had a higher growth rate, higher photosynthetic activity and potential, and significantly greater stress resistance than the other seedlings. Therefore, appropriate shading measures were beneficial to the cultivation of vigorous seedlings. Furthermore, spectral reflectance indexes were found to be a suitable tool for monitoring the photosynthetic physiological characteristics, stress resistance characteristics, and growth status of P. massoniana seedlings in real time.
Spatiotemporal Prescribed Fire Patterns in Washington State, USA
Authors: Podschwit Harry , Miller Colton , Alvarado Ernesto
Published: April 2021

We investigate the spatiotemporal patterns of prescribed fire and wildfire within Washington State, USA using records from the state’s Department of Natural Resources (DNR). Spatiotemporal comparisons of prescribed fire and wildfire area burned revealed that (1) fire activity broadly differed between the eastern and western portion of the state in terms of total area and distribution of burn sources, (2) over the 2004–2019 period, wildfire largely replaced prescribed fire as the predominant source of burning, and (3) wildfire and prescribed fire occur during distinct months of the year. Spatiotemporal variation in prescribed fire activity at regional levels were measured using five parameters: total area burned, total biomass burned, burn days, burn approval rates, and pile burn frequency. Within-region spatial variability in prescribed fire parameters across land ownership categories and bioclimatic categories were often detectable. Regression models of the annualized prescribed fire parameters suggested that prescribed fire activities have been declining in multiple administrative regions over the 2004–2019 period. A descriptive analysis of seasonal trends found that prescribed fire use largely peaked in the fall months, with minor peaks usually occurring in the spring. Lastly, we described how area burned, biomass burned, and pile burn frequency differed between prescribed fires approved and denied by the DNR, and found that approved prescribed fires were typically smaller and burned less biomass than denied fires.
Customising Evacuation Instructions for High-Rise Residential Occupants to Expedite Fire Egress: Results from Agent-Based Simulation
Authors: Gerges Michael , Demian Peter , Adamu Zulfikar
Published: April 2021

As the possibility of safe escape is one of the most crucial aspects of a building’s fire safety features, understanding of human behaviour under fire conditions is important for a successful evacuation. Although most of today’s buildings are equipped with fire safety systems, a fire can still occur at anytime and anywhere in a building and have devastating consequences. In the last decade, researchers and practitioners have used information technology to assist with fire safety design and emergency management. Building Information Modelling (BIM) is an exemplar process whose underpinning digital technology has been helpful for fire safety design, simulation, and analysis, but there is a lack of research on how BIM-based models combined with agent-based simulations can help improve evacuation via effective navigation and wayfinding in high-rise residential buildings. Customising evacuation instructions based on BIM, simulation results and occupant location, and delivery of these bespoke instructions to occupants’ smartphones during a fire emergency is relatively novel and research is needed to realise the potential of this approach. Therefore, this study investigates how customised evacuation instructions delivered to each occupant in a high-rise residential building could result in a faster evacuation during a fire incident. The research adopted a case study building and used Pathfinder (agent-based evacuation simulation software) to simulate evacuation from this eleven-floor high-rise residential building in Cairo, Egypt. Constraining evacuees (simulated agents in Pathfinder) to take particular exit routes was used as a proxy for delivering customised evacuation instructions to actual evacuees. Simulation results show that, in general, allowing the use of lifts for the benefit of disabled occupants could lead to their misuse by able-bodied occupants; evacuees would attempt to use the first visible point of exit regardless of how crowded it is. With optimally customised instructions, the evacuation time was, on average, 17.6 min (almost 50%) shorter than when the occupant’s choice of egress route was simulated based on standard path planning factors such as route length, nearby crowds and visible hazards. With evacuation instructions sent via smartphones, occupants could exit more rapidly via alternative routes. Such bespoke instructions were shown to reduce the adverse effects of crowdedness and uneven distribution of occupants along vertical and horizontal evacuation routes on evacuation time.
Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise
Authors: Liang Xiao , Wan Ying , Shen Zhiyuan , Liu Yanmei , Li Dapeng , Li Li , Tang Rong , Zhang Xi
Published: April 2021

Heat shock protein 47 (Hsp47) is a collagen-specific molecular chaperone that is indispensable for molecular maturation of collagen. In this study, hsp47 and hsp47-like cDNAs were cloned and characterized in grass carp (Ctenopharyngodon idella). The cDNAs were 1212 and 1218 base pairs long, respectively, and included an open reading frame encoding 403 and 405 amino acids. The molecular phylogeny based on the deduced amino acid sequences indicated that the correct sequences of the hsp47 and hsp47-like cDNA were obtained and the deduced proteins clustered distinctly into teleost clades. Primary structure analysis and characterization of Hsp47 and Hsp47-like shared the basic structure and biofunctions of Hsp47 in vertebrates. The spatial pattern of gene expression revealed that hsp47 and hsp47-like were relatively ubiquitous in different tissues and highly expressed in heart and skin. The expression levels of hsp47 and hsp47-like and type I collagen mRNAs varied similarly in different tissues. Type I collagen content increased significantly with the increase of water velocity in the muscle of grass carp in response to aerobic exercise. Among the gene expression changes of hsp47, hsp47-like, col1a1 and col1a2 in muscle that occurred in response to aerobic exercise, the change of type I collagen was most strongly correlated with hsp47 expression. Additionally, col1a1 showed the highest correlation with hsp47-like and col1a2 showed the highest correlation with hsp47. These findings suggest that grass carp Hsp47 and Hsp47-like are closely related to type I collagen synthesis. This study firstly suggests fish aerobic exercise can improve type I collagen content and Hsp47 gene expression in muscle of grass carp.
The Occurrence of Glycosylated Aroma Precursors in Vitis vinifera Fruit and Humulus lupulus Hop Cones and Their Roles in Wine and Beer Volatile Aroma Production
Authors: Caffrey Andrew , Ebeler Susan E.
Published: April 2021

Volatile aroma compounds found in grapes and hops may be present as both free volatiles and bound glycosides. Glycosides found in the raw materials are transferred to their respective fermented beverages during production where the odorless compounds may act as a reservoir of free volatiles that may be perceived by the consumer if hydrolyzed. A review of the literature on grape and wine glycosides and the emerging literature for glycosides in hops is presented in order to demonstrate the depth of history in grape glycoside research and may help direct new research on hop glycosides. Focus is brought to the presence of glycosides in the raw materials, the effect that winemaking and brewing have on glycoside levels, and current methods for the analysis of glycosidically linked aroma compounds.
Nutrient Composition and Antioxidant Performances of Bread-Making Products Enriched with Stinging Nettle (Urtica dioica) Leaves
Authors: Maietti Annalisa , Tedeschi Paola , Catani Martina , Stevanin Claudia , Pasti Luisa , Cavazzini Alberto , Marchetti Nicola
Published: April 2021

Stinging nettle (Urtica dioica) is an edible plant, well-known for its nutritional and nutraceutical properties. Stinging nettle leaves are typically rich in fibers, minerals and vitamins, as well as antioxidant compounds, i.e., polyphenols and carotenoids. Due to these reasons, since ancient times stinging nettle has been widely used in Italy as an ingredient in foods and beverages as a therapeutic agent. This work provides an investigation focused on bread enrichment with nettle leaves and the improvement of bread proximate composition in minerals, fibers and antioxidant compounds during product preparation. The comparison between plain and nettle enriched white bread shows a significant increase in fibers and nutrients, i.e., calcium and copper levels. Nettle enrichment also provides an increase in lutein and β-carotene, as well as in total phenols and antioxidant activity. These last two nutritional elements are remarkably high in enriched bread and it has been found that phenolic concentration increases during breadmaking steps, from kneading to primary dough fermentation and from secondary fermentation of shaped loaves to baking.
Using Naturalistic Driving Data to Predict Mild Cognitive Impairment and Dementia: Preliminary Findings from the Longitudinal Research on Aging Drivers (LongROAD) Study
Authors: Di Xuan , Shi Rongye , DiGuiseppi Carolyn , Eby David W. , Hill Linda L. , Mielenz Thelma J. , Molnar Lisa J. , Strogatz David , Andrews Howard F. , Goldberg Terry E. , Lang Barbara H. , Kim Minjae , Li Guohua
Published: April 2021

Emerging evidence suggests that atypical changes in driving behaviors may be early signals of mild cognitive impairment (MCI) and dementia. This study aims to assess the utility of naturalistic driving data and machine learning techniques in predicting incident MCI and dementia in older adults. Monthly driving data captured by in-vehicle recording devices for up to 45 months from 2977 participants of the Longitudinal Research on Aging Drivers study were processed to generate 29 variables measuring driving behaviors, space and performance. Incident MCI and dementia cases (n = 64) were ascertained from medical record reviews and annual interviews. Random forests were used to classify the participant MCI/dementia status during the follow-up. The F1 score of random forests in discriminating MCI/dementia status was 29% based on demographic characteristics (age, sex, race/ethnicity and education) only, 66% based on driving variables only, and 88% based on demographic characteristics and driving variables. Feature importance analysis revealed that age was most predictive of MCI and dementia, followed by the percentage of trips traveled within 15 miles of home, race/ethnicity, minutes per trip chain (i.e., length of trips starting and ending at home), minutes per trip, and number of hard braking events with deceleration rates ≥ 0.35 g. If validated, the algorithms developed in this study could provide a novel tool for early detection and management of MCI and dementia in older drivers.
Exploring the Effects of “Smart City” in the Inner-City Fabric of the Mediterranean Metropolis: Towards a Bio-Cultural Sonic Diversity?
Authors: Kyvelou Stella Sofia , Bobolos Nicos , Tsaligopoulos Aggelos
Published: April 2021

“Smart city”, driven by digital technology is not only a technological but also a social, cultural and political project. A socially and culturally significant new urban ideal is born. This research paper is based on the narrative that the city appears as a palimpsest of interventions of all natures. History and shared memory, composition and superimposition, coherence and divergence are fundamental for its evolution. It is thus evident that ”Smart city” as a rather new urban ideal, but also as a disruptive innovation process, cannot be conceived nor implemented all at once; it must follow analogous processes of buildup and stratification. On the other hand, sounds are part of cities, of their sensory landscape, of their identity. They are one of the urban markers, along with the visual landscape. In this context, the paper focuses on the sound identity of the inner-city areas of the Mediterranean metropolis, posing the following research question: What are the transformations that “Smart city” can cause to the sound identity of a city? In dense urban fabric with high-rise buildings, high rates of exploitation, frequent transgressions of the legal construction and least free space in private plots, what can be the prospects of using “smart transport”, for enriching the city with positive soundscapes, thus improving its environmental quality? Following the metaphor of urban and acoustic palimpsest, we examine narratives of replacement of conventional cars with autonomous vehicles (AVs) and of private cars with car-pooling systems. The article concludes that spatialized intelligence can substantially and positively transform the sound identity of the Mediterranean metropolis and be the spearhead for an increase in bio-cultural sonic diversity. At least during the era when the city still appears as a palimpsest of interpositions, evoking the historic time.