bluuebunny/crossref_metadata_2025 huggingface dataset:
Percontohan taman toga serta produksi jamu berbasis tanaman berkhasiat untuk peningkatan kesehatan dan ekonomi masyarakat
Authors: Rahardjo Djoko , Mularum Seta Nurhayati , Madyaningrana Kukuh , Ariestanti Catarina Aprilia , Prasetyaningsih Aniek , Widayanti Nurlia
Published: July 2022

Tanaman berkhasiat obat banyak dimanfaatkan oleh masyarakat di Indonesia secara turun-temurun untuk mendukung kesehatan. Inventarisasi dan pemanfaatan beragam tanaman obat mutlak dilakukan untuk pengembangan potensinya dalam menunjang kesehatan. Pemanfaatan jamu sebagai produk olahan tanaman obat mulai mendapatkan perhatian lebih dari pemerintah melalui institusi kesehatan. Pengabdian ini bertujuan untuk membuat percontohan taman tanaman obat keluarga (Toga) di Dusun Ngelosari, Kecamatan Piyungan, Kabupaten Bantul yang memadukan rintisan ekowisata lereng bukit dan pengolahan tanaman obat keluarga. Kegiatan pengabdian ini didasarkan pada analisis situasi dan permasalahan kesehatan di lingkup Puskesmas Piyungan. Berdasarkan identifikasi masalah dan analisis kebutuhan yang dilakukan bersama puskesmas dan kader kesehatan maka pendekatan pengembangan percontohan taman toga, pelatihan dan pendampingan pada kelompok wanita tani (KWT) dipilih sebagai pendekatan yang diharapkan mampu menyelesaikan permasalahan kesehatan dan ekonomi masyarakat. Program terealisir dalam bentuk persiapan dan observasi lokasi taman Toga, pengembangan taman Toga percontohan, pelatihan budidaya tanaman obat, pasca panen dan pengolahan, serta pengemasan dan pemasaran produk herbal. Kegiatan ini mempunyai hasil berupa Taman Toga Ngupoyo Sehat yang menjadi sumber bahan baku produk jamu yang dihasilkan oleh KWT setempat. Selain meningkatkan pendapatan KWT setempat, pelaksanaan program dapat dipergunakan sebagai model sinergisme pengembangan konservasi dan peningkatan kesehatan masyarakat.
Penggunaan Drill Method dalam Perilaku Hidup Bersih dan Sehat Sebagai Pencegahan COVID-19
Authors: Merdekawati Diah , Astuti Ani , Puspita Mefrie
Published: July 2022

Peningkatan kualitas kesehatan masyarakat dapat diaplikasikan melalui dengan penerapan perilaku hidup bersih dan sehat (PHBS). Salah satu masalah yang ditemukan yaitu kondisi dan lingkungan mitra (Rumah Yatim YMAJU) kurang memadai serta belum ada penerapan PHBS. Untuk itu, Program Kemitraan Masyarakat (PKM) ini dilakukan dengan tujuan untuk meningkatkan kemampuan mitra dalam menerapkan PHBS. Solusi yang diberikan berupa edukasi tentang PHBS. Tujuan PHBS menjadikan peningkatan kualitas perilaku hidup bersih dan sehat pada sebagian besar masyarakat. Melalui PHBS tiap individu menjadi sadar dan ikut serta dalam menerapkan perilaku di kehidupan sehari-hari. Sasaran penyuluhan PHBS ini adalah anak yatim dibawah binaan Yayasan Mutiara Abadi Jariah Umat. Peningkatan pengetahuan dan keterampilan peserta dilakukan dengan drill method berupa penyuluhan PHBS, demonstrasi dan simulasi mencuci tangan. Tahap evaluasi diperoleh hasil peningkatan pengetahuan dan keterampilan peserta tentang PHBS dan mencuci tangan. Pengetahuan dan keterampilan PHBS yang baik akan menjadi dasar bagi peserta terhindar dari penularan COVID-19.
Politik Hukum Dalam Penanganan Covid-19: Pendekatan Yuridis Dalam Sistem Hukum Indonesia
Authors: Mahardika Ahmad Gelora
Published: July 2022

The condition of the economic and health dilemma in handling covid-19 is due to regulations in the Indonesian legal system forcing the Government to choose to sacrifice one of them. However, the compromising legal politics carried out by the Government has a tendency to deviate from the applicable law. Therefore, a new legal policy is needed in handling the covid-19 pandemic outbreak, including the issuance of a PERPU as legitimacy to provide legal certainty for a number of policies carried out by the Government that deviate from the applicable laws and regulations. The research method in this article is normative juridical. The issuance of PERPU is the most appropriate legal policy to overcome problems related to the covid-19 pandemic which is trapped between economic and health dilemmas.
Perbandingan Hukum Internasional Dan Nasional Tentang Hak Asasi Manusia
Authors: Efendi Muhammad Fadli
Published: July 2022

Human rights are rights that must be upheld, guaranteed and protected by law, the state, the government and other human beings. It cannot be reduced, revoked, or even eliminated, because these rights are inherent in humans from the moment they were created (baby in the womb). Cases of human rights violations in the world are so rife in various countries, even rarely that countries do not violate human rights. So it is necessary to make an improvement effort in protecting and guaranteeing human rights. This study aims to provide a complete understanding of human rights through normative juridical research methods with a statutory, comparative, and historical approach. From this research, it is found that there are visible differences ranging from the definition of human rights, mechanisms for resolving human rights violations, to institutions for resolving human rights violations both in international of law and national of law in Indonesia.
Analisis Kerusakan Pada Mesin Batching Plant di PT. Duta Borneo Abadi Menggunakan Metode Hazard and Operability Study (HAZOP)
Authors: Sulistianingtyas Dita Arum , Putrianto Novenda Kartika
Published: July 2022

PT. Duta Borneo Abadi adalah sebuah perusahaan yang menghasilkan semen cor dalam skala besar sebagai produk utama mereka. Dalam proses produksinya, mesin utama yang digunakan adalah mesi n batching plant. Hal tersebut menyebabkan mesin batching plan memiliki potensi kerusakan yang dapat menyebabkan breakdown, sehingga diperlukan adanya identifikasi serta analisis menggunakan metode Hazard and Operability Study (HAZOP). HAZOP merupakan teknik untuk mengidentifikasi dan menganalisis penyimpangan pada sebuah proses operasi mesin. Data yang digunakan pada penelitian ini adalah data kerusakan mesin, data maintenance mesin, data pipe and instrument diagram, dan data process flow diagram. Berdasarkan observasi yang telah dilakukan diperoleh 6 titik studi (node) pada mesin batching plant guna mempermudah mengidentifikasi hazard yang timbul, yaitu zona penyimpanan agregat, zona penimbangan, zona penyaluran, zona penyimpanan semen, zona penyimpanan dan penyaluran air, dan zona pencampuran. Hasil analisis hazard dengan menggunakan worksheet HAZOP menunjukan terdapat 17 potensi bahaya, terdiri dari 15 bahaya beresiko rendah dan 2 bahaya beresiko tinggi. Berdasarkan potensi resiko yang terjadi, diperlukan rekomendasi safeguard pada setiap bahaya yang ditimbulkan dan usulan pembuatan daily checksheet untuk mengecek komponen mesin setiap bulan sehingga dapat menghindari timbulnya hazard.
ANALISIS PERANCANGAN USAHA PEMANFAATAN LIMBAH PRODUKSI TEMPE DI UMKM AMANAH SANAN MALANG
Authors: Mahardika Bagus Kurniawan , Hadi Yuswono
Published: July 2022

Tempe merupakan salah satu industri olahan kedelai yang banyak dijumpai diberbagai wilayah di indonesia. Melihat banyaknya permintaan akan olahan tersebut tentu potensi limbah yang dihasilkan juga semakin banyak. Limbah yang dimaksud dalam olahan ini yaitu limbah kedelai, limbah kedelai ini terbagi menjadi dua jenis yaitu limbah kulit ari kedelai dan limbah cair air rebusan kedelai. Dalam penelitian yang dilakukan di Umkm Amanah dimana setiap harinya terdapat sekitar 50-60 kg limbah padat dan > 45 liter limbah cair yang dihasilkan Dalam upaya meminimalisir limbah serta meningkatkan pendapatan usaha umkm maka dilakukan pengolahan terhadap limbah menjadi sebuah usaha sampingan yaitu usaha produk Tepung Kulit Kedelai dan Nata de soya dengan metode analisis perancangan usaha. Pengumpulan data seputar limbah dilakukan dengan cara wawancara dan observasi dengan pemilik Umkm Amanah. Setelah data seputar limbah didapatkan dilakukan observasi dan penelitian terkait upaya pengolahan limbah menja di sebuah produk jadi. Untuk aspek finasisial rancangan bisnis usaha ini memiliki nilai payback period (PP) selama 2.58 tahun. Untuk keputusan layak tidaknya maka dapat diambil kesimpulan untuk perancangan usaha dianggap layak dikembangkan oleh Umkm Amanah hal itu dikarenakan dengan melihat perhitungan nilai NPV (Net Present Value) yaitu $42.321.728,56 > 0 dan IRR (Internal Rate of Return) yang lebih besar dari Marr yaitu 56 % > 17.1% maka sangat berpotensi untuk dijalankan. Sehingga penelitian ini diharapk an menjadi pondasi rancangan dasar yang dimiliki Umkm Amanah untuk mengembakan proyek ini secara rill di kedepannya
PERANCANGAN SISTEM PENGENDALIAN KUALITAS DENGAN MENGGUNAKAN PENDEKATAN SPC DI PT. X
Authors: Marcelieno Matthew , Ekawati Yurida
Published: July 2022

Abstrak. PT. X merupakan perusahaan yang bergerak dalam bidang industri minuman berperasa dengan merek dagang Y. Permasalahan yang dihadapi perusahaan adalah belum adanya sistem pengendalian untuk mengontrol rata-rata dan variasi dari suatu produk, dan besarnya angka produk cacat yang dialami selama beberapa bulan yang lalu. Berdasarkan persentase cacat yang dimiliki perusahaan, pada bulan September 2019 – Desember 2020 terdapat persentase cacat sebesar 0.419%. Perancangan sistem pengendalian kualitas dilakukan untuk membuat suatu dokumen sistem pengendalian kualiatas yang dapat dimanfaatkan oleh perusahaan dalam mengendalikan kualitas produknya. Business process chart digunakan untuk memetakan sistem pengendalian kualitas. Sistem pengendalian kualitas usulan digambarkan dengan business process chart dengan pendekatan statistical process control (SPC). Checksheet digunakan untuk membantu mengambil data. Peta kontrol digunakan untuk mengkontrol rata-rata dan variasi dari volume, pH, brix, dan produk cacat. Pareto digunakan untuk menganalisis data 8 potensi penyebab produk cacat. Selain itu, dilakukan juga upaya penyelesaian terhadap masalah yang saat ini muncul yaitu permasalahan produk cacat yang disebabkan cup penyok dan lid bocor. Perbaikan yang dilakukan untuk mengurangi produk cacat ternyata memiliki hasil positif dan berhasil menurunkan produk cacat. Sebuah perba ikan dan sistem usulan telah dimplementasikan dan diketahui proses rata-rata dan variasi dari volume, pH, dan brix terkendali/incontrol. Sistem pengendalian kualitas yang diusulkan disajikan dalam bentuk Standard Operating Procedures (SOP)
Temperature mediates chorusing behaviour associated with spawning in the sciaenid Argyrosomus regius
Authors: Vieira M , Amorim MCP , Marques TA , Fonseca PJ
Published: July 2022

Climate change is leading to an increase in the frequency and intensity of daily temperature extremes in aquatic environments, posing a threat to ectothermic animals such as fishes. Temperature shifts can impact their reproductive cycle and behaviour, including acoustic signalling associated with courtship and mating in vocal fishes. Here, we examined the effect of temperature and other environmental factors on spawning-related chorusing behaviour in the meagreArgyrosomus regius. Using an automatic recognition system based on hidden Markov models coupled with one-third octave band sound level analysis, we extracted meagre daily vocal activity over 4 yr of passive acoustic monitoring (PAM) in the Tagus estuary (Portugal). Meagre vocal activity showed a distinct diel pattern, with choruses being most prevalent at dusk. Chorus activity started earlier in years with warmer springs, while the end of the season was concurrent with the rise in water temperature. Circa 70% of daily variations were explained by changes in water temperature. Tidal cycle, lunar phase and changes in daylength did not appear to affect vocal behaviour. Choruses were detected on days with mean water temperature ranging from 15-25°C, with stronger chorusing observed around 18°C. Abrupt temperature changes caused disruption in choruses and likely in associated spawning. This study highlights the importance of temperature to fish reproduction and demonstrates that PAM can be a useful tool for long-term conservation management plans. PAM may also allow us to predict how temperature shifts due to climate change may affect fish reproduction, using vocal fishes as model species.
TELESCOPIC NUMERICAL SEMIGROUPS WITH MULTIPLICITY TEN AND EMBEDDING DIMENSION THREE
Authors: SÜER Meral , İLHAN Sedat
Published: July 2022

In this work, we give parametrizations of telescopic numerical semigroups with multiplicity ten and embedding dimension three.

We also express some of its invariants in terms of generators of these semigroups such as the Frobenius number, genus and Sylvester number.
A Systematic Review of Program Outcomes for the National Family Caregiver Support Program Using a Theoretical Approach
Authors: Ivey Dorothea L.
Published: July 2022

The National Family Caregiver Support Program provides grants to states with large populations of citizens ages 70 and older that are formal and informal caregivers to fund support for various services. The Child Welfare Information Gateway (2016) defined kinship caregiving as the full-time care and protection of children by relatives. The purpose of this study is to identify outcomes of state and community level programs initiated by the National Family Caregiver Support Program (NFCSP) that serves informal grandparent caregivers. Micro and macro frameworks of rational decision making, symbolic interaction, and agency theories were used in this study when determining program outcomes. Outcomes reported in this study were: (1) reduced isolation, (2) increased connection to resources, (3) increased independence/empowerment, (4) reduced stress, (5) lower levels of burden, (6) increased well-being, and (7) intergenerational reciprocity. The NFCSP is a significant first step in assisting this population and is an important federal program that provides much needed funding to Area Agencies on Aging (AAA) in support of grandparent raising grandchildren.
Comportamento ortoréxico em estudantes de Nutrição
Authors: Rodrigues Alexandra Magna , Dargham Munira Mohamad , Oliveira Adriana Mônica Pinto de
Published: July 2022

Problema: Estudantes de nutrição apresentam comportamento de risco para o desenvolvimento de Ortorexia Nervosa.  Objetivo: Identificar comportamento de risco para o desenvolvimento de Ortorexia Nervosa em estudantes de nutrição. Métodos: Estudo transversal realizado com estudantes do sexo feminino do curso de Nutrição com idade igual ou maior que 18 anos do interior paulista, Brasil. Aplicou-se um questionário com informações sobre idade, estatura, peso autorreferidos e ano do curso; a Escala de Silhuetas para avaliar a percepção da imagem corporal e o ORTO 15 para identificar comportamento ortoréxico. Resultados: Participaram da pesquisa 182 alunas. Verificou-se que 88,5% apresentaram comportamento ortoréxico, independentemente da idade e ano cursado (p>0,05). Observou-se que 71,4% estavam eutróficas e que 34% apresentaram insatisfação com imagem corporal. Entretanto, não houve associação do comportamento ortoréxico com o estado nutricional e com a percepção da imagem corporal (p>0,05). Conclusão: A maioria das estudantes de nutrição apresentaram comportamento ortoréxico, independente do ano cursado, estado nutricional, percepção corporal ou idade. Tais achados apontam a necessidade de outros estudos sobre a complexa e ainda pouco elucidada temática da Ortorexia Nervosa, especialmente entre os profissionais de Nutrição que deveriam ter uma relação adequada, e não obsessiva com o alimento, considerando os aspectos biológicos, sociais e culturais da alimentação saudável.
TRANSGRESSÃO, CONHECIMENTO E FÉ: SOM E LUZ IMORTALIZANDO AS CONFISSÕES DE SANTO AGOSTINHO SOB A PERSPECTIVA DE ROBERTO ROSSELLINI
Authors: Conconi Thais , Morales Martinez David
Published: July 2022

Neste artigo buscamos contextualizar o cinema como fonte de conhecimento, pois, uma vez que esta linguagem pode condensar diversas visões acerca de um fenômeno, ela se apresentará como meio para o desenvolvimento de uma postura crítica sobre a relação cinema e história, bem como os objetos que permeiam este discurso. Analisamos o filme Agostino d’Ippona (1972) da coleção "Os Filósofos", dirigida por Roberto Rossellini, buscando, através do olhar cinematográfico do diretor, reconstruir a história do bispo e a evolução de seus pensamentos, numa perspectiva filosófica. Após a análise fílmica feita sobre com base nas transcrições de diálogos, imagens, luz e banda sonora, foi feita uma reflexão sobre os conceitos de ciências, filosofia e religião presentes na obra, dentre eles o tempo, a criação, a liberdade e a dualidade existente entre o bem e o mal e, desta forma, mostramos que o uso do cinema explorado criticamente de forma ampliada, ou seja, através dos símbolos e signos que estruturam uma base de valores estéticos e éticos em algum contexto temático e cultural, pode potencializar a construção de novos saberes, além de imortalizar obras que se constituem como representações de um tempo passado.
A SINGULARIDADE DA FUGA DO OLHAR DE DANIELA: A REPRESENTAÇÃO DO REAL NA LENTE DO DOCUMENTARISTA COUTINHO?
Authors: Uhry Ricardo , Torres Andacht Fernando
Published: July 2022

O objetivo do artigo é verificar, por meio da análise de um episódio, se um documentário pode transmitir uma ideia da representação do real, para o que se utiliza de estudo de caso. Como resultado, sugere-se que o encontro documental provoca efeitos no espectador a partir da interação entre o diretor (registrada pela câmara) e uma pessoa qualquer. Por meio da leitura e análise semiótica do episódio de Daniela frente ao diretor Eduardo Coutinho (e a câmara) no filme Edifício Master, evidenciam-se singularidades que podem ser relacionadas ao real. O encontro documental nos mostra algo da imensa singularidade que é o Outro, portanto pode ser considerado uma fonte para esse conhecimento parcial e falível produzido pela representação documentária, que é muito precioso ao nos desvendar algo sobre o mistério da alteridade e indicar algo que, através do signo interpretante, pode ter efeito “balsâmico” e ser considerado analiticamente como um exemplo de “index appeal” e, em termos peirceanos, de “haecceidade”.
“ARCHÉ” LUDENS: O JOGO COMO IMAGEM “DESNORMATIVA” DE/PARA UMA TRANSFIGURAÇÃO ESTÉTICA
Authors: Postiga Ivan , Loureiro Domingos
Published: July 2022

O presente ensaio propõe, num quadro atual, uma conceptualização exploratória das relações entre – “Arte” e “Jogo”. Em diálogo com diversas perspetivas teóricas, ensaia o modo como o “jogo”, a partir do início do séc. XX, mais do que um elemento "interno" do conceito de “arte”, seguindo a tradição filosófica, se tornou num modelo "externo", isto é, num espaço estratégico de “experi-mentação radical”, capaz de funcionar como - seu catalisador criativo, - seu dispositivo ou contra-dispositivo representacional, - seu modelo operativo de projeção autoral, reinvenção metodológica e transformação estética.
TROCAS ENTRE ARTE CONTEMPORÂNEA E “CULTURA POPULAR”: CARLOS VERGARA E O CACIQUE DE RAMOS
Authors: Freitas Lima Pedro Ernesto
Published: July 2022

A constatação da operacionalidade da arte, especialmente a arte contemporânea, no “campo ampliado da cultura”, dado a impossibilidade de tratá-la em termos de autonomia e muito menos de esteticismo, como propôs Hal Foster (2014, p. 174), demandam trabalhos e pesquisas que busquem investigar como produções artísticas se relacionam com outros campos, como aquele denominado de “cultura popular”. Em Carnaval-ritual: Carlos Vergara e Cacique de Ramos (2021), o pesquisador e professor Maurício Barros de Castro parte da relação do artista visual Carlos Vergara (1941) com o carnaval de rua do Rio de Janeiro, particularmente o bloco carnavalesco Cacique de Ramos (1961), para propor uma abordagem possível que considere, para além de diálogos, trânsitos e imbricamentos entre arte contemporânea e “cultura popular”. Trata-se aqui de abordar a “cultura popular” não como tema para a arte, como de certo modo caracterizou parte de nossa produção modernista. Diferentemente, Castro propõe pensarmos metodologicamente a partir de outros modos, nos quais se compreende essas manifestações integradas aos processos de criação e ao desenvolvimento poético do artista.
A AÇÃO DE ATITUDE TRANSDISCIPLINAR E DE CRIATIVIDADE COLETIVA NO ESPETÁCULO PERFORMÁTICO CHAOS DAS 5
Authors: de Andrade Morandi Thiago , Luiz Schiavoni Flávio
Published: July 2022

A discussão das artes pode atualmente permear outras áreas para além da discussão estética e ser incorporada por exemplo, na sustentabilidade. Esta abertura do campo parte de um conceito revisitado do que significa sustentabilidade e nos leva para uma discussão de transdisciplinaridade como forma de ampliar o espaço das artes e suas relações interartes. É diante deste cenário que apresentamos a obra performática Chaos da 5 que conta com a participação de estudantes de graduação e pós-graduação de várias áreas do conhecimento, que se uniram para realizar uma construção conjunta que aplica conceitos teóricos e metodológicos de suas áreas no processo criativo da performance.
Allelopathy: an eco-friendly approach to control palmer amaranth using allelopathic sweetpotato
Authors: Singh Varsha , Segbefia Worlanyo , Fuller Mary Gracen , Shankle Mark W. , Morris Callie J. , Meyers Stephen L. , Tseng Te-Ming
Published: July 2022

Palmer amaranth (PA) is one of the major weeds in sweetpotato reducing its quantity and quality. The widespread and repeated use of chemical herbicides has led PA to develop resistance for such chemicals. In addition, chemical herbicides are incompatible with the organic production system. It is imperative to find sustainable weed management strategies to provide weed control suitable for organic cultivation and detain the development of herbicide-resistant weeds under conventional crop production. In the present study, seventeen sweetpotato varieties were screened for their allelopathic (weed-suppressing) effect on the growth of PA. The experiment was conducted in a greenhouse using a stair-step system. Each plant in the stair-step system had its height (cm), chlorophyll concentration (cci) and shoot biomass (g) measured. The variation in the height, chlorophyll and shoot biomass reduction of PA was significant after the third week of transplanting. Three weeks after transplanting (WAT), only three varieties, i.e., Morado (75%), Bayou belle (62%), and Vardaman (61%), reduced PA’s height by >60% compared to the control. While 5 WAT, four varieties, i.e., 529 (93%), Morado (93%), Heartogold (85%), and Centennial (81%), reduced PA height by >80%. Hatteras, Centennial, and 529 reduced the chlorophyll content of PA by >50%. In the presence of Beauregard, the commercial cultivar, there was no reduction in shoot biomass of PA. Cluster analysis also demonstrated that the four allelopathic sweetpotato varieties, i.e., Heart-O-Gold, Centennial, 529, and Morado, were clustered together, indicating that these varieties have similar potential to suppress the growth and development of PA. Combining allelopathic sweetpotato cultivars (Heart-O-Gold, Centennial, 529, and Morado) with other sustainable weed control measures, such as cover crops and hand-weeding, can improve the weed management, espicially in organic farming. However, field experiments should be conducted to confirm the allelopathic as well as yield potential of these varieties in an agronomic setting. The availability of the allelopathic sweetpotato cultivars will benefit organic producers by enhancing crop productivity and decrease reliance on chemical herbicides in conventional farming systems.
In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes
Authors: Hassane Abdallah M. A. , Hussien Saleh M. , Abouelela Mohamed E. , Taha Taher M. , Awad Mohamed F. , Mohamed Hassan , Hassan Mohammad M. , Hassan Mohammad H. A. , Abo-Dahab Nageh F. , El-Shanawany Abdel-Rehim A.
Published: July 2022

Oxidative stress is involved in the pathophysiology of multiple health complications, and it has become a major focus in targeted research fields. As known, black seeds are rich sources of bio-active compounds and widely used to promote human health due to their excellent medicinal and pharmaceutical properties. The present study investigated the antioxidant potency of various black seeds from plants and their derived mycoendophytes, and determined the total phenolic and flavonoid contents in different extracts, followed by characterization of major constituents by HPLC analysis. Finally, in silico docking determined their binding affinities to target myeloperoxidase enzymes. Ten dominant mycoendophytes were isolated from different black seed plants. Three isolates were then selected based on high antiradical potency and further identified by ITS ribosomal gene sequencing. Those isolated were Aspergillus niger TU 62, Chaetomium madrasense AUMC14830, and Rhizopus oryzae AUMC14823. Nigella sativa seeds and their corresponding endophyte A. niger had the highest content of phenolics in their n-butanol extracts (28.50 and 24.43 mg/g), flavonoids (15.02 and 11.45 mg/g), and antioxidant activities (90.48 and 81.48%), respectively, followed by Dodonaea viscosa and Portulaca oleracea along with their mycoendophytic R. oryzae and C. madrasense. Significant positive correlations were found between total phenolics, flavonoids, and the antioxidant activities of different tested extracts. The n-butanol extracts of both black seeds and their derived mycoendophytes showed reasonable IC50 values (0.81–1.44 mg/ml) compared to the control with significant correlations among their phytochemical contents. Overall, seventeen standard phenolics and flavonoids were used, and the compounds were detected in different degrees of existence and concentration in the examined extracts through HPLC analysis. Moreover, the investigation of the molecular simulation results of detected compounds against the myeloperoxidase enzyme revealed that, as a targeted antioxidant, rutin possessed a high affinity (−15.3184 kcal/mol) as an inhibitor. Taken together, the black seeds and their derived mycoendophytes are promising bio-prospects for the broad industrial sector of antioxidants with several valuable potential pharmaceutical and nutritional applications.
Nuclear Localization Signals for Optimization of Genetically Encoded Tools in Neurons
Authors: Karasev Maksim M. , Baloban Mikhail , Verkhusha Vladislav V. , Shcherbakova Daria M.
Published: July 2022

Nuclear transport in neurons differs from that in non-neuronal cells. Here we developed a non-opsin optogenetic tool (OT) for the nuclear export of a protein of interest induced by near-infrared (NIR) light. In darkness, nuclear import reverses the OT action. We used this tool for comparative analysis of nuclear transport dynamics mediated by nuclear localization signals (NLSs) with different importin specificities. We found that widely used KPNA2-binding NLSs, such as Myc and SV40, are suboptimal in neurons. We identified uncommon NLSs mediating fast nuclear import and demonstrated that the performance of the OT for nuclear export can be adjusted by varying NLSs. Using these NLSs, we optimized the NIR OT for light-controlled gene expression for lower background and higher contrast in neurons. The selected NLSs binding importins abundant in neurons could improve performance of genetically encoded tools in these cells, including OTs and gene-editing tools.
Link Between Antibiotic Persistence and Antibiotic Resistance in Bacterial Pathogens
Authors: Eisenreich Wolfgang , Rudel Thomas , Heesemann Jürgen , Goebel Werner
Published: July 2022

Both, antibiotic persistence and antibiotic resistance characterize phenotypes of survival in which a bacterial cell becomes insensitive to one (or even) more antibiotic(s). However, the molecular basis for these two antibiotic-tolerant phenotypes is fundamentally different. Whereas antibiotic resistance is genetically determined and hence represents a rather stable phenotype, antibiotic persistence marks a transient physiological state triggered by various stress-inducing conditions that switches back to the original antibiotic sensitive state once the environmental situation improves. The molecular basics of antibiotic resistance are in principle well understood. This is not the case for antibiotic persistence. Under all culture conditions, there is a stochastically formed, subpopulation of persister cells in bacterial populations, the size of which depends on the culture conditions. The proportion of persisters in a bacterial population increases under different stress conditions, including treatment with bactericidal antibiotics (BCAs). Various models have been proposed to explain the formation of persistence in bacteria. We recently hypothesized that all physiological culture conditions leading to persistence converge in the inability of the bacteria to re-initiate a new round of DNA replication caused by an insufficient level of the initiator complex ATP-DnaA and hence by the lack of formation of a functional orisome. Here, we extend this hypothesis by proposing that in this persistence state the bacteria become more susceptible to mutation-based antibiotic resistance provided they are equipped with error-prone DNA repair functions. This is - in our opinion - in particular the case when such bacterial populations are exposed to BCAs.
Modelling complex molecular interactions in catalytic materials for energy storage and conversion in nuclear magnetic resonance
Authors: Hu Wenda , Jaegers Nicholas R. , Winkelman Austin D. , Murali Shiva , Mueller Karl T. , Wang Yong , Hu Jian Zhi
Published: July 2022

Nuclear magnetic resonance (NMR) is a non-destructive and atom-specific specific tool that has become a burgeoning analytic method for understanding the detailed molecular interactions in catalysis and energy storage materials. However, the observation of diverse chemical shifts arising from complex molecular interactions makes the interpretation of NMR spectroscopy increasingly challenging, in particular for a novel system without standards for comparison. Density functional theory-NMR (DFT-NMR) is an indispensable tool to mitigate these challenges and provide detailed 3D molecular structures that relate materials and reaction intermediate structures, and information about chemical interactions, dynamics, and reaction mechanisms. This review provides a fundamental background in DFT-NMR relating to theory development, critical parameters for calculating NMR properties, computational accuracy, and the current capabilities. A variety of practical examples from the fields of catalysis and energy storage, including CO2 capture, are summarized to illustrate the capabilities of DFT-NMR application to date. Last but not least, cautionary notes on the application of these strategies are presented for researchers modeling their own systems.
Surgical treatment of giant right ventricular fibroma for a newborn: A case report
Authors: Liu Yuhang , Wang Ning , Wen Ping
Published: July 2022

This report describes the surgical treatment of giant right ventricular fibroma in a newborn. Cardiac uhrasonography and CT showed a large mass in the right ventricle wall, which narrowed the right ventricular inflow tract. The newborn patient gradually developed symptoms such as shortness of breath, oliguria, and pericardial effusion. We performed tumor excision, but due to severe damage to the right ventricular wall and right heart failure, the patient relied on cardiopulmonary bypass. Then, we immediately restored the opening of the ductus arteriosus, enlarged the foramen ovale, and used various vasoactive drugs to ensure the smooth resuscitation of the patient. This is a kind of operation for the youngest patients. The perioperative treatment experience indicated the feasibility of excision of giant right ventricular fibroma for newborn patients.
Physical Model Test on the Deformation Mechanism of Reservoir Bank Slopes With Sand Layers Under Coupled Hydro-Mechanical Conditions
Authors: Zhu Junhua , Lv Qing , Xie Dian , Zeng Runzhong , Wang Ning , Qiao Jianwei , Cai Weibin
Published: July 2022

A reservoir area is mostly located in the canyon area, and the geological structure is complex. There are a large number of unstable slopes on the bank of the reservoir. The stability of bank slope is greatly affected by water storage and reservoir water regulation. In addition, sudden rainstorm and other external factors can reduce slope stability. In this article, the physical model test is used to study the seepage field and deformation characteristics of typical reservoir bank slopes with sand layers under different rainfall intensities, different water level fluctuation rates, and their coupling effects. The model has a length of 4.0 m, a width of 1.0 m, and a height of 0.9 m, and the piezometers and white balls are used to monitor the pore water pressures and displacements inside the slope model individually. The results show that the responsiveness of pore water pressure inside the slope lags behind both water level fluctuation and rainfall. The lag time is inversely proportional to the water level fluctuation rates under the single water level fluctuation condition, while it is proportional to water level fluctuation rates in the water level decline stage under the coupling effect condition. The rapid impoundment of the reservoir area has a strengthening effect on the stability of the reservoir bank slope. However, accelerated deformation of the slope occurs in the stage of water level decline, and the deformation rate is proportional to the water level fluctuation rates.
Fracture Imaging Using DAS-Recorded Microseismic Events
Authors: Staněk František , Jin Ge , Simmons James
Published: July 2022

Hydraulic fracturing enables hydrocarbon production from unconventional reservoirs. Mapping induced seismicity around newly created fractures is crucial for understanding the reservoir response and increasing the efficiency of operations. Distributed acoustic sensing (DAS) provides a large amount of high spatial resolution microseismic data acquired along the entire length of horizontal wells. We focus on the observed reflected S-waves and develop a new methodology using microseismic events as sources of energy to image induced fractures acting as reflectors in the media surrounding the events and monitoring fiber. The workflow consists of DAS data preprocessing, event location, wavefield separation, raytracing-based imaging, and image post-processing. The comparison of the resulting images with low-frequency DAS signals of fracture hits corroborates that the reflections are from fractures created by stimulation. The proposed algorithm can be used for real-time mapping of fractures and tracking fracture changes in space and time. Fracture imaging leads to a better understanding of the reservoir response to hydraulic fracturing stimulation.
Case Report: Progression of a Silent Corticotroph Tumor to an Aggressive Secreting Corticotroph Tumor, Treated by Temozolomide. Changes in the Clinic, the Pathology, and the β-Catenin and α-SMA Expression
Authors: Demarchi Gianina , Perrone Sofía , Esper Romero Gaela , De Bonis Cristian , Casasco Juan Pablo , Sevlever Gustavo , Berner Silvia Ines , Cristina Carolina
Published: July 2022

Clinically silent corticotroph tumors are usually macroadenomas that comprise 20% of ACTH tumors. They frequently progress to aggressive tumors with high recurrence, invasiveness, and on rare occasions, they may become hormonally active causing Cushing’s disease. Trustable biomarkers that can predict their aggressive course, as well as their response to traditional or new therapies, are paramount. Aberrant β-Catenin expression and localization have been proposed as responsible for several malignancies including pituitary tumors. Nevertheless, the role of β-Catenin in the aggressive transformation of silent corticotropinomas and their response to Temozolomide salvage treatment have not been explored yet. In this work, we present a case of a silent corticotroph tumor that invaded cavernous sinus and compressed optic chiasm and, after a first total resection and tumor remission it recurred six years later as an aggressive ACTH-secreting tumor. This lesion grew with carotid compromise and caused Cushing’s signs. It required multiple medical treatments including Cabergoline, Ketoconazole, TMZ, and radiotherapy. Besides, other two surgeries were needed until it could be controlled. Interestingly, we found α-SMA vascular area reduction and differential β-Catenin cell localization in the more aggressive tumor stages characterized by high Ki-67 indexes and p53 expression. Our results may indicate a role of angiogenesis and β-Catenin trigged events in the pituitary tumor progression, which could in turn affect the response to TMZ and/or conventional treatments. These molecular findings in this unusual case could be useful for future management of aggressive pituitary tumors.
Present and Future: Crosstalks Between Polycystic Ovary Syndrome and Gut Metabolites Relating to Gut Microbiota
Authors: Zhang Mingmin , Hu Runan , Huang Yanjing , Zhou Fanru , Li Fan , Liu Zhuo , Geng Yuli , Dong Haoxu , Ma Wenwen , Song Kunkun , Song Yufan
Published: July 2022

Polycystic ovary syndrome (PCOS) is a common disease, affecting 8%–13% of the females of reproductive age, thereby compromising their fertility and long-term health. However, the pathogenesis of PCOS is still unclear. It is not only a reproductive endocrine disease, dominated by hyperandrogenemia, but also is accompanied by different degrees of metabolic abnormalities and insulin resistance. With a deeper understanding of its pathogenesis, more small metabolic molecules, such as bile acids, amino acids, and short-chain fatty acids, have been reported to be involved in the pathological process of PCOS. Recently, the critical role of gut microbiota in metabolism has been focused on. The gut microbiota-related metabolic pathways can significantly affect inflammation levels, insulin signaling, glucose metabolism, lipid metabolism, and hormonal secretions. Although the abnormalities in gut microbiota and metabolites might not be the initial factors of PCOS, they may have a significant role in the pathological process of PCOS. The dysbiosis of gut microbiota and disturbance of gut metabolites can affect the progression of PCOS. Meanwhile, PCOS itself can adversely affect the function of gut, thereby contributing to the aggravation of the disease. Inhibiting this vicious cycle might alleviate the symptoms of PCOS. However, the role of gut microbiota in PCOS has not been fully explored yet. This review aims to summarize the potential effects and modulative mechanisms of the gut metabolites on PCOS and suggests its potential intervention targets, thus providing more possible treatment options for PCOS in the future.
Surrogate Model of Predicting Eigenvalue and Power Distribution by Convolutional Neural Network
Authors: Zhang Jinchao , Zhou Yufeng , Zhang Qian , Wang Xiang , Zhao Qiang
Published: July 2022

During loading pattern (LP) optimization and reactor design, a lot of time consumption spent on evaluation is one of the key issues. In order to solve this issue, the surrogate models are investigated in this paper. The convolutional neural network (CNN) and fully convolutional network (FCN) are adopted to predict the eigenvalue and the assembly-wise power distribution (PD) for a simplified pressurized water reactor (PWR) during depletion, respectively. For the eigenvalue prediction during depletion, the error in the begin of cycle (BOC) and middle of cycle (MOC) is higher than that in the end of cycle (EOC). For the BOC and MOC, the samples with discrepancy over 500 pcm are less than 1%, except four burnup points. For the EOC, the fraction of samples with error over 500 pcm is less than 1%. As for the error of assembly power, the average absolute error is on the same level for all test cases. The average absolute relative error in the center region and the peripheral region is higher than that in the inter-ring region. The prediction results indicate the capability of neural network to predict core parameters.
Hierarchical Optimization and Grid Scheduling Model for Energy Internet: A Genetic Algorithm-Based Layered Approach
Authors: Lin Lihua , Abdallah Abdallah , Ishak Mohamad Khairi , Ali Ziad M. , Khan Imran , Rabie Khaled , Safak Bayram Islam , Li Xingwang , Madsen Dag Øivind , Kim Ki-Il
Published: July 2022

The old economic and social growth model, characterized by centralized fossil energy consumption, is progressively shifting, and the third industrial revolution, represented by new energy and Internet technology, is gaining traction. Energy Internet, as a core technology of the third industrial revolution, aims to combine renewable energy and Internet technology to promote the large-scale use and sharing of distributed renewable energy as well as the integration of multiple complex network systems, such as electricity, transportation, and natural gas. This novel technology enables power networks to save energy. However, multienergy synchronization optimization poses a significant problem. As a solution, this study proposed an optimized approach based on the concept of layered control–collaborate optimization. The proposed method allows the distributed device to plan the heat, cold, gas, and electricity in the regional system in the most efficient way possible. Moreover, the proposed optimization model is simulated using a real-number genetic algorithm. It improved the optimal scheduling between different regions and the independence of distributed equipment with minimal cost. Furthermore, the inverse system and energy and cost saving rate of the proposed method are better than those of existing methods, which prove its effectiveness.
The prospected air quality measurements for further unconventional natural gas developments in China based on the United States experience
Authors: Fang Tao , Zhang Xiaomeng , Zhang Jie , Wang Junfeng , Sun Qianqian
Published: July 2022

The technological innovation of horizontal drilling and high-volume hydraulic fracturing has promoted the development of unconventional natural gas (UNG) production worldwide, and hence has aroused public concern about the air pollution it may bring about. In this study, we have provided (1) an overview of the study on air pollutants from UNG emissions in the USA, focusing on both the air pollutant characterization and their related observation technologies/platforms; and (2) the potential air quality measurements of UNG development emerging in China. This study will provide useful information for Chinese environmental researchers and the local governments to deal with related air quality issues.
Immediate and Sustained Effects of Cobalt and Zinc-Containing Pigments on Macrophages
Authors: Devcic Julie , Dussol Manon , Collin-Faure Véronique , Pérard Julien , Fenel Daphna , Schoehn Guy , Carrière Marie , Rabilloud Thierry , Dalzon Bastien
Published: July 2022

Pigments are among the oldest nanoparticulate products known to mankind, and their use in tattoos is also very old. Nowadays, 25% of American people aged 18 to 50 are tattooed, which poses the question of the delayed effects of tattoos. In this article, we investigated three cobalt [Pigment Violet 14 (purple color)] or cobalt alloy pigments [Pigment Blue 28 (blue color), Pigment Green 14 (green color)], and one zinc pigment [Pigment White 4 (white color)] which constitute a wide range of colors found in tattoos. These pigments contain microparticles and a significant proportion of submicroparticles or nanoparticles (in either aggregate or free form). Because of the key role of macrophages in the scavenging of particulate materials, we tested the effects of cobalt- and zinc-based pigments on the J774A.1 macrophage cell line. In order to detect delayed effects, we compared two exposure schemes: acute exposure for 24 hours and an exposure for 24 hours followed by a 3-day post-exposure recovery period. The conjunction of these two schemes allowed for the investigation of the delayed or sustained effects of pigments. All pigments induced functional effects on macrophages, most of which were pigment-dependent. For example, Pigment Green 19, Pigment Blue 28, and Pigment White 4 showed a delayed alteration of the phagocytic capacity of cells. Moreover, all the pigments tested induced a slight but significant increase in tumor necrosis factor secretion. This effect, however, was transitory. Conversely, only Pigment Blue 28 induced both a short and sustained increase in interleukin 6 secretion. Results showed that in response to bacterial stimuli (LPS), the secretion of tumor necrosis factor and interleukin 6 declined after exposure to pigments followed by a recovery period. For chemoattractant cytokines (MCP-1 or MIP-1α), delayed effects were observed with a secretion decreased in presence of Pigment Blue 28 and Pigment violet 14, both with or without LPS stimuli. The pigments also induced persisting changes in some important macrophage membrane markers such as CD11b, an integrin contributing to cell adhesion and immunological tolerance. In conclusion, the pigments induced functional disorders in macrophages, which, in some cases, persist long after exposure, even at non-toxic doses.
Spatial Distribution and Encounter Rates of Delphinids and Deep Diving Cetaceans in the Eastern Mediterranean Sea of Turkey and the Extent of Overlap With Areas of Dense Marine Traffic
Authors: Awbery Tim , Akkaya Aylin , Lyne Patrick , Rudd Laura , Hoogenstrijd Gemma , Nedelcu Madeleine , Kniha David , Erdoğan Mehmet Akif , Persad Cherisse , Amaha Öztürk Ayaka , Öztürk Bayram
Published: July 2022

Marine traffic has been identified as a serious threat to Mediterranean cetaceans with few mitigation strategies in place. With only limited research effort within the Eastern Basin, neither baseline species knowledge nor the magnitude of threats have been comprehensively assessed. Delineating the extent of overlap between marine traffic and cetaceans provides decision makers with important information to facilitate management. The current study employed the first seasonal boat surveys within the Eastern Mediterranean Sea of Turkey, incorporating visual and acoustic survey techniques between 2018 and 2020 to understand the spatial distribution of cetacean species. Additionally, marine traffic density data were retrieved to assess the overlap with marine traffic. Encounter rates of cetaceans and marine traffic density were recorded for each 100 km2cell within a grid. Subsequently, encounter and marine traffic density data were used to create a potential risk index to establish where the potential for marine traffic and cetacean overlap was high. Overall, eight surveys were undertaken with a survey coverage of 21,899 km2between the Rhodes and Antalya Basins. Deep diving cetaceans (sperm and beaked whales) were detected on 28 occasions, with 166 encounters of delphinids of which bottlenose, striped and common dolphins were visually confirmed. Spatially, delphinids were distributed throughout the survey area but encounter rates for both deep diving cetaceans and delphinids were highest between the Rhodes and Finike Basins. While sperm whales were generally detected around the 1000m contour, delphinids were encountered at varying depths. Overall, two years of monthly marine traffic density were retrieved with an average density of 0.37 hours of monthly vessel activity per square kilometer during the study period. The mean density of vessels was 0.32 and 1.03 hours of monthly vessel activity per square kilometer in non-coastal and coastal waters respectively. The Eastern Mediterranean Sea has several important shipping lanes within the study area. Two priority areas for deep diving cetacean and a large priority area for cetaceans were identified in the waters between Marmaris and Finike where high cetacean encounters and dense marine traffic overlapped. The current study revealed important habitats for cetaceans within the data deficient Eastern Mediterranean Sea and delineated potential risk area where marine traffic should be limited.