bluuebunny/crossref_metadata_2025 huggingface dataset:
Editorial for the Special Issue on “Advances in Middle Infrared Laser Crystals and Its Applications”
Authors: Duan Xiaoming , Dou Renqin , Li Linjun , Yang Xiaotao
Published: April 2022

In the past two decades, there has been a growing interest in middle infrared (mid-IR) laser crystals and its application to achieve mid-IR laser radiations, which has been benefited by the development of novel mid-infrared crystals and the improving quality of traditional mid-IR crystals [...]
Analytical Validation of GFRNMR: A Blood-Based Multiple Biomarker Assay for Accurate Estimation of Glomerular Filtration Rate
Authors: Fuhrmann Markus , Schwaeble Santamaria Amauri , Scott Renee , Meeusen Jeffrey W. , Fernandes Marianna , Venz John , Rothe Victoria , Stämmler Frank , Ehrich Jochen , Schiffer Eric
Published: April 2022

Accurate and precise monitoring of kidney function is critical for a timely and reliable diagnosis of chronic kidney disease (CKD). The determination of kidney function usually involves the estimation of the glomerular filtration rate (eGFR). We recently reported the clinical performance of a new eGFR equation (GFRNMR) based on the nuclear magnetic resonance (NMR) measurement of serum myo-inositol, valine, and creatinine, in addition to the immunoturbidometric quantification of serum cystatin C, age and sex. We now describe the analytical performance evaluation of GFRNMR according to the Clinical and Laboratory Standards Institute guidelines. Within-laboratory coefficients of variation (CV%) of the GFRNMR equation did not exceed 4.3%, with a maximum CV% for repeatability of 3.7%. Between-site reproducibility (three sites) demonstrated a maximum CV% of 5.9%. GFRNMR stability was demonstrated for sera stored for up to 8 days at 2–10°C and for NMR samples stored for up to 10 days in the NMR device at 6 ± 2°C. Substance interference was limited to 4/40 (10.0%) of the investigated substances, resulting in an underestimated GFRNMR (for glucose and metformin) or a loss of results (for naproxen and ribavirin) for concentrations twice as high as usual clinical doses. The analytical performances of GFRNMR, combined with its previously reported clinical performance, support the potential integration of this NMR method into clinical practice.
A Feasibility Study of Developing eLCV Shared Architecture in Taiwan
Authors: Wei I-Hua , Wang Fu-Ming , Chang Chung-Hao
Published: April 2022

Vehicle electrification has become an important strategy adopted worldwide, including in Taiwan, as a means to achieving net zero emissions. Taiwan is capable of building a whole light commercial vehicle and has technological strength in producing critical EV parts. This study applies the Bass diffusion model to assess the feasibility of developing eLCV shared architecture in Taiwan and estimates that the annual replacement demand for eLCVs could reach 20,221 units. This exceeds the threshold number of 5000 units, which could motivate the automakers to develop eLCV shared architecture. The simulation result shows that achieving full market penetration would take at least 13 years and would be highly correlated with policy support, the vehicle selling price and the battery pack price. The B2B model is a suitable way of introducing eLCVs into the logistics fleets. In the initial promotion phase, policy support and complementary measures would be needed, e.g., public sectors’ purchases, financial incentives and constructing a user-friendly environment. The simulation results further indicate that Taiwan’s eLCV market has a high price elasticity, and in the future, a tendency for the battery pack price to decline may speed up the replacement process.
Adaptive Neural Network Global Nonsingular Fast Terminal Sliding Mode Control for a Real Time Ground Simulation of Aerodynamic Heating Produced by Hypersonic Vehicles
Authors: Lv Xiaodong , Zhang Guangming , Zhu Mingxiang , Ouyang Huimin , Shi Zhihan , Bai Zhiqing , Alexandrov Igor V.
Published: April 2022

This paper presents a strategy for a thermal-structural test with quartz lamp heaters (TSTQLH), combined with an ultra-local model, a closed-loop controller, a linear extended state observer (LESO), and an auxiliary controller. The TSTQLH is a real time ground simulation of aerodynamic heating for hypersonic vehicles to optimize their thermal protection systems (TPS). However, lack of a system dynamic model for the TSTQLH results in inaccurate tracking of aerodynamic heating. In addition, during the control process, the TSTQLH has internal uncertainties of resistance and external disturbances. Therefore, it is necessary to establish a mathematical model between controllable α(t) and measurable T1(t). An ultra-local model of model-free control plays a crucial role in simplifying system complexity and reducing high-order terms due to high nonlinearities and strong couplings in the system dynamic model, and a global nonsingular fast terminal sliding mode control (GNFTSMC) is added to an ultra-local model, which is used to guarantee great tracking performance in the sliding phase and fast convergence to the equilibrium state in finite time. Moreover, the LESO is used mainly to estimate all disturbances in real time, and an adaptive neural network (ANN) shows a good approximation property in compensation for estimation errors by using a cubic B-spline function. The fitted curve of the wall temperature in the time sequence represents a reference temperature trajectory from the surface contour of an X-43A’s wing. The comparative results validate that the proposed control strategy possesses strong robustness to track the reference temperature trajectory.
Experimental Study of the Effect of Air Filter Pressure Drop on Internal Combustion Engine Performance
Authors: Dziubak Tadeusz , Karczewski Mirosław
Published: April 2022

The paper presents the problem of the effect of air filter pressure drop on the operating parameters of a modern internal combustion engine with compression ignition. A literature analysis of the results of investigations of the effect of air filter pressure drop on the filling, power and fuel consumption of carburetor and diesel engines with classical injection system was carried out. It was shown that each increase in the air filter pressure drop Δpf by 1 kPa results in an average decrease in engine power by SI 1–1.5% and an increase in specific fuel consumption by about 0.7. For compression ignition engines, the values are 0.4–0.6% decrease in power and 0.3–0.5% increase in specific fuel consumption. The values of the permissible resistance of the air filter flow Δpfdop determined from the condition of 3% decrease in engine power are given, which are at the level of 2.5–4.0 kPa—passenger car engines, 4–7 kPa—truck engines and 9–12 kPa—special purpose vehicles. Possibilities of decreasing the pressure drop of the inlet system, which result in the increase of the engine filling and power, were analyzed. The program and conditions of dynamometer engine tests were worked out in respect to the influence of the air filter pressure drop on the operation parameters of the six-cylinder engine of the swept volume Vss = 15.8 dm3 and power rating of 226 kW. Three technical states of the air filter were modeled by increasing the pressure drop of the filter element. For each technical state of the air filter, measurements and calculations of engine operating parameters, including power, hourly and specific fuel consumption, boost pressure and temperature, were carried out in the speed range n = 1000–2100 rpm. It was shown that the increase in air filter pressure drop causes a decrease in power (9.31%), hourly fuel consumption (7.87%), exhaust temperature (5.1%) and boost pressure (3.11%). At the same time, there is an increase in specific fuel consumption (2.52%) and the smoke of exhaust gases, which does not exceed the permissible values resulting from the technical conditions for admission of vehicles to traffic.
One Earth Climate Model—Integrated Energy Assessment Model to Develop Industry-Specific 1.5 °C Pathways with High Technical Resolution for the Finance Sector
Authors: Teske Sven , Guerrero Jaysson
Published: April 2022

According to the IPCC, a global carbon budget of 400 GtCO2 is required to limit the temperature rise to 1.5 °C with a 67% likelihood by 2050. The finance industry is increasingly committed to ambitious climate targets. In this article, we describe the detailed methodology and energy model architecture of a MATLAB-based integrated energy assessment model for industry-specific 1.5 °C pathways, with a high technical resolution of target parameters as key performance indicators (KPIs). The additionality of OECM 2.0 is the high technical resolution in terms of the level of detail of industry-specific energy demand and supply parameters that can be modeled—a prerequisite to define industry-specific KPIs. We found that a database of industry-sector-specific energy demands and energy intensities, with a consistent methodology, is required to improve the accuracy of calculations in future research. We supplement the technical documentation with the results for a transport scenario.
Cdh1 Deficiency Sensitizes TNBC Cells to PARP Inhibitors
Authors: Li Junjun , Lan Mengjiao , Peng Jin , Xiong Qunli , Xu Yongfeng , Yang Yang , Zhou Ying , Liu Jinlu , Zeng Zhu , Yang Xiaojuan , Zhang Zhiwei , Zhang Pumin , Zhu Qing , Wu Wei
Published: April 2022

Triple-negative breast cancer (TNBC) is a type of breast tumor that currently lacks options for targeted therapy. Tremendous effort has been made to identify treatment targets for TNBC. Here, we report that the expression level of anaphase promoting complex (APC) coactivator Cdh1 in TNBC is elevated compared to that in the adjacent healthy tissues, and high levels of Cdh1 expression are correlated with poor prognoses, suggesting that Cdh1 contributes to the progression of TNBC. Interfering with the function of Cdh1 can potentiate the cytotoxic effects of PARP inhibitors against BRCA-deficient and BRCA-proficient TNBC cells through inducing DNA damage, checkpoint activation, cell cycle arrest, and apoptosis. Further investigation reveals that Cdh1 promotes BRCA1 foci formation and prevents untangled DNA entering mitosis in response to PARP inhibition (PARPi) in TNBC cells. Collectively, these results suggest that APC/Cdh1 is a potential molecular target for PARPi-based therapies against TNBCs.
New Sex Chromosomes in Lake Victoria Cichlid Fishes (Cichlidae: Haplochromini)
Authors: Kocher Thomas D. , Behrens Kristen A. , Conte Matthew A. , Aibara Mitsuto , Mrosso Hillary D. J. , Green Elizabeth C. J. , Kidd Michael R. , Nikaido Masato , Koblmüller Stephan
Published: April 2022

African cichlid fishes harbor an extraordinary diversity of sex-chromosome systems. Within just one lineage, the tribe Haplochromini, at least 6 unique sex-chromosome systems have been identified. Here we focus on characterizing sex chromosomes in cichlids from the Lake Victoria basin. In Haplochromis chilotes, we identified a new ZW system associated with the white blotch color pattern, which shows substantial sequence differentiation over most of LG16, and is likely to be present in related species. In Haplochromis sauvagei, we found a coding polymorphism in amh that may be responsible for an XY system on LG23. In Pundamilia nyererei, we identified a feminizing effect of B chromosomes together with XY- and ZW-patterned differentiation on LG23. In Haplochromis latifasciatus, we identified a duplication of amh that may be present in other species of the Lake Victoria superflock. We further characterized the LG5-14 XY system in Astatotilapia burtoni and identified the oldest stratum on LG14. This species also showed ZW differentiation on LG2. Finally, we characterized an XY system on LG7 in Astatoreochromis alluaudi. This report brings the number of distinct sex-chromosome systems in haplochromine cichlids to at least 13, and highlights the dynamic evolution of sex determination and sex chromosomes in this young lineage.
Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts
Authors: Yang Sha , Yang Yuze , Hao Haisheng , Du Weihua , Pang Yunwei , Zhao Shanjiang , Zou Huiying , Zhu Huabin , Zhang Peipei , Zhao Xueming
Published: April 2022

The quality and developmental capacity of oocytes derived from in vitro maturation (IVM) remain unsatisfactory, which greatly impairs the efficiency and application of embryo technologies. The present experiment was designed to investigate the effect of the supplementation of EGF, IGF-1, and Cx37 in an IVM medium on the maturation quality and development ability of bovine oocytes. The cytoplasmic maturation events of oocytes and the quality of in vitro fertilization (IVF) blastocysts were examined to investigate the relative mechanisms. Our results showed that the nuclear maturation and blastocyst development after the IVF of oocytes treated with 25 μg/mL Cx37 or the combination of 50 ng/mL EGF and 100 ng/mL IGF-1 were significantly increased compared to those of the control group (p < 0.05). Furthermore, the blastocyst rate, and blastocyst total cell number and survival rate after vitrification of the EGF+IGF-1+Cx37 group, were significantly higher than those of the control group (p < 0.05), but lower than those of the FSH+LH+EGF+IGF-1+Cx37 group (p < 0.05). The transzonal projection (TZP) intensity, glutathione (GSH) level, and mitochondrial function of the EGF+IGF-1+Cx37 group were significantly higher than that of the control group, and lower than those of the FSH+LH+EGF+IGF-1+Cx37 group, in contrast to the results of the reactive oxygen species (ROS) levels. In conclusion, our results showed that the supplementation of 50 ng/mL EGF, 100 ng/mL IGF-1, and 25 μg/mL Cx37 in the IVM of bovine oocytes significantly improved their quality and developmental ability by increasing the TZP, mitochondrial function, and GSH level.
Remote-Controlled Drone System through Eye Movements of Patients Who Need Long-Term Care: An Intermediary’s Role
Authors: Betriana Feni , Tanioka Ryuichi , Kogawa Atsunori , Suzuki Riku , Seki Yuki , Osaka Kyoko , Zhao Yueren , Kai Yoshihiro , Tanioka Tetsuya , Locsin Rozzano
Published: April 2022

The use of a remote-controlled drone system (RDS) by eye movements was studied to assist patients in psychiatric long-term care (PLTC) to allow them to view the environment outside the hospital, hoping that this will bring them some enjoyment. However, successfully applying this system requires human intermediaries in facilitating the interactions between patients and RDS operators. The aim of the study was to describe the role of nurses as intermediaries in the application of an RDS through eye movements of patients PLTC. This study employed the Intentional Observational Clinical Research Design. Data collection was performed in November 2021 at a psychiatric hospital with selected patients in PLTC. Seventeen patients took part in the indoor experiment, whereas 23 patients took part in the outdoor experiment. Fifteen of the 23 patients in the outdoor experiment were the same patients who took part in the indoor experiment. Most of the patients in the indoor and outdoor test arenas could successfully, delightfully, and safely fly the drone. This study demonstrated that RDS using just eye movements could increase the quality of life in older patients with psychiatric problems in PLTC. For the successful use of this drone system, nurse intermediaries assumed critically significant roles.
Stabilization of the Distal Radioulnar Joint with or without Triangular Fibrocartilage Complex Tear by an External Wrist Band Brace: A Cadaveric Study
Authors: Shin Seung-Han , Park Taeyong , Hong Eunah , Kwak Dai-Soon , Chung Yang-Guk
Published: April 2022

The purpose of this study was to investigate whether a watch-shaped external wrist band brace improves distal radioulnar joint (DRUJ) stability. Seven fresh cadaveric arms were used. Using a customized testing system, volar and dorsal translation forces were applied to the radius externally while the ulna was fixed. The test was performed with the forearm in neutral, 60° pronated, and 60° supinated positions, once without the brace and once with the brace applied. In each condition, the amount of translation was measured. Then, the triangular fibrocartilage complex (TFCC) was detached from the ulnar styloid process and the fovea ulnaris, and the same tests were performed again. Detachment of the TFCC significantly increased volar and dorsal translations in all forearm rotations compared to the intact condition (p < 0.05), except for the pronated dorsal translation of the radius (p = 0.091). Brace application significantly reduced volar and dorsal translations in all forearm rotations both in intact specimens and in TFCC-detached specimens (p < 0.05), except for pronated volar and dorsal translations in TFCC-detached specimens (p = 0.101 and p = 0.131, respectively). With the brace applied, the TFCC-detached specimens showed no significant difference in volar or dorsal translation in all forearm rotations compared to the intact specimens (p > 0.05). The external wrist band brace improved DRUJ stability in both normal and TFCC-torn wrists and reduced the DRUJ instability caused by TFCC tear to a near-normal level.
Provoked Vestibulodynia and Topical Treatment: A New Option
Authors: De Seta Francesco , Stabile Guglielmo , Antoci Graziana , Zito Gabriella , Nappi Rossella E.
Published: April 2022

Background: Provoked vestibulodynia is commonly associated with dyspareunia and affects 7% to 15% of women. This pathology has major implications on sexual function and quality of life, and several types of treatments are available for its management. However, a consensus has not been reached concerning the best treatment of vulvar pain. The aim of this study was to assess the efficacy and safety of a brand-new product, the vulvar emulgel Meclon® Lenex, for the management of provoked vestibulodynia and non-infective vulvitis. Methods: This was a monocentric, prospective, randomized, double-blind and placebo-controlled study. We enrolled 40 women with provoked vestibulodynia; 20 patients received Meclon® Lenex, whereas the remaining received a placebo. Each woman was assessed subjectively (through questionnaires) and objectively by evaluating vaginal and vulvar symptoms (Friedrichs criteria and Marinoff dyspareunia grade). We evaluated efficacy, safety, compliance and tolerability of the brand-new product vulvar gel Meclon® Lenex in provoked vestibulodynia. Results: After administration of Meclon® Lenex, we evaluated all parameters of the Friedrichs criteria (burning, dyspareunia, erythema, vulvar pain at the 5 o’clock position and 7 o’clock position), as well as the levels of Marinoff dyspareunia. The active treatment showed to be statistically significantly effective (p value ≤ 0.05) in reducing all symptoms of Friedrichs criteria, vulvar pain and Marinoff dyspareunia. Conclusion: This prospective study showed that Meclon® Lenex vulvar emulgel revealed an excellent tolerability and compliance, demonstrating to be a safe and effective option in the treatment of provoked vestibulodynia and non-infective vulvitis.
Shared Care Practices in Community Addiction and Mental Health Services: A Qualitative Study on the Experiences and Perspectives of Stakeholders
Authors: Foster Michele , Weaver Julia , Shalaby Reham , Eboreime Ejemai , Poong Kimberly , Gusnowski April , Snaterse Mark , Surood Shireen , Urichuk Liana , Agyapong Vincent I. O.
Published: April 2022

Shared care involves collaboration between primary care, secondary and tertiary care that enables the allocation of responsibilities of care according to the treatment needs of patients over the course of a mental illness. This study aims to determine stakeholders’ perspectives on the features of an ideal shared care model and barriers to practicing shared care within addiction and mental health programs in Edmonton, Canada. This is a qualitative cross-sectional study with data collected through focus group discussions. Participants included patients, general practitioners, psychiatrists, management, and therapists working in primary and secondary addiction and mental health. Responses were audio-recorded, transcribed, and analyzed thematically. Perceived barriers to the implementation of an ideal shared care model identified by participants include fragmented communication between primary and secondary healthcare providers, patient and family physician discomfort with discussing addiction and mental health, a lack of staff capacity, confidentiality issues, and practitioner buy-in. Participants also identified enablers to include implementing shared electronic medical record systems, improving communication and collaboration, physical co-location, and increasing practitioner awareness of appropriate referrals and services. This original research provides stakeholders’ perspectives on the features of an ideal shared care model and barriers to practicing shared care within addiction and mental health programs.
Hop: An Emerging Crop in Subtropical Areas in Brazil
Authors: Jastrombek Jessiane Mary , Faguerazzi Mariana Mendes , de Cássio Pierezan Hyan , Rufato Leo , Sato Alessandro Jefferson , da Silva Ricce Wilian , Marques Viviani Vieira , Leles Nathalia Rodrigues , Roberto Sergio Ruffo
Published: April 2022

Brazil is one of the three largest beer producers in the world. Four basic ingredients are needed as raw material for the production of beer: water, malt, yeast, and hops (Humulus lupulus L.). Until recently, almost all of the hops in Brazil were imported from other countries. However, in the last decade, hop cultivation in Brazil has emerged due to the increase in the number of new craft breweries, which have demanded diversified raw material for the production of various types of beer. Hops is considered a short-day, temperate species, so the major challenge for the development of hop cultivation in Brazil, with high-yield capacity and with local typicity of bitterness and aroma, is the adaptation of cultivars to the photoperiod conditions in subtropical regions. This review addresses the history of hop cultivation in Brazil and characterizes the main climatic elements of three emerging subtropical growing regions located at different latitudes, such as air temperature, photoperiod, solar radiation, and water availability, to provide support for the development of new technologies for hop cultivation, including supplemental lighting, irrigation, and mulching.
The Association between Multiple Per- and Polyfluoroalkyl Substances’ Serum Levels and Allostatic Load
Authors: Bashir Tahir , Obeng-Gyasi Emmanuel
Published: April 2022

Background/Objective: This study aimed to explore the association between allostatic load (AL), an index of chronic stress, with nine per- and polyfluoroalkyl substances (PFASs), a group of organic compounds used in commercial and industrial applications. The PFASs explored were perfluorohexane sulfonic acid (PFHS), perfluorodecanoic acid (PFDE), perfluorobutane sulfonic acid (PFBS), perfluoroheptanoic acid (PFHP), perflurododecanoic acid (PFDO), perfluorononanoic acid (PFNA), perfluoroundecanoic acid (PFUA), perfluorooctanoic acid (PFOA), and perfluorooctane sulfonic acid (PFOS). This study was performed to better understand the association between PFASs and AL, which may be a mediator of several diseases. Methods: This study was performed on adults aged 20 and older, using the National Health and Nutrition Examination Survey (NHANES) 2007–2014 data. AL was calculated as a cumulative index of ten biomarkers from the cardiovascular, inflammatory, and metabolic system, which was dichotomized into high risk (assigned a value of 1) or low risk (assigned a value 0) depending on if the index value was ≥3 (chronic physiological stress) or <3 (less stressed). In this study, PFASs and covariates such as age, gender, ethnicity, alcohol consumption, smoking, and physical activity were explored using descriptive statistics and logistic regression modeling. Results: The results indicated that in adults, AL was more elevated in men as compared to women, in those aged ≥60 years, and varied by ethnicity. For instance, non-Hispanic Blacks had higher AL levels (mean of 3.92) compared to other ethnicities. A significant number of the participants tested for PFBS, PFHP, PFDO were below the LOD and thus these PFASs were excluded from the analysis. Our analysis demonstrated multicollinearities between variables such as PFNA, PFOS, and PFOA with variance inflation factor (VIF) values of 6.197, 6.212, and 5.139, respectively. Thus, PFASs were analyzed individually and adjusted for age, gender, ethnicity, physical activity, smoking, and alcohol consumption. The results indicated a statistically significant positive association between AL and most of the PFASs, except PFUA which was not statistically significant with a p value of 0.531. Conclusions: The findings of this study suggest that exposure to PFDE, PFNA, PFOS, PFOA, and PFHS are associated with AL when adjusted for age, gender, ethnicity, alcohol consumption, smoking, and physical activity. Future studies looking to model the effects of these factors together must consider their relationship with each other and choose different analytical approaches.
Assessing Opioid Use Disorder Treatments in Trials Subject to Non-Adherence via a Functional Generalized Linear Mixed-Effects Model
Authors: St. Ville Madeleine , Bergen Andrew W. , Baurley James W. , Bible Joe D. , McMahan Christopher S.
Published: April 2022

The opioid crisis in the United States poses a major threat to public health due to psychiatric and infectious disease comorbidities and death due to opioid use disorder (OUD). OUD is characterized by patterns of opioid misuse leading to persistent heavy use and overdose. The standard of care for treatment of OUD is medication-assisted treatment, in combination with behavioral therapy. Medications for opioid use disorder have been shown to improve OUD outcomes, including reduction and prevention of overdose. However, understanding the effectiveness of such medications has been limited due to non-adherence to assigned dose levels by study patients. To overcome this challenge, herein we develop a model that views dose history as a time-varying covariate. Proceeding in this fashion allows the model to estimate dose effect while accounting for lapses in adherence. The proposed model is used to conduct a secondary analysis of data collected from six efficacy and safety trials of buprenorphine maintenance treatment. This analysis provides further insight into the time-dependent treatment effects of buprenorphine and how different dose adherence patterns relate to risk of opioid use.
The Moderating Effect of Compassionate Mindfulness on the Psychological Needs and Emotions of Generation Y in the 21st Century in Taiwan
Authors: Lin Hui-Li , Lin Fang-Suey , Liu Ling-Chen , Liu Wen-Hsin
Published: April 2022

During the 2020 COVID-19 pandemic in Taiwan, 6.5% of Generation Y required medical treatment for emotional and stress-related mental disorders. This study explores the moderating effect of mindfulness training on psychological needs and emotions to propose effective measures to promote the mental health of Generation Y. This study was carried out by questionnaire, using the data of respondents born in 1980–1999, collected in three different periods for quantitative analysis with compassionate mindfulness as the main variable. The results show that the compassionate mindfulness effect on emotion regulation varies greatly among different educational levels. However, it still plays a positive role in the psychological needs of Generation Y. Most members of Generation Y who receive compassionate mindfulness training have fewer basic needs and more interpersonal trust. They pay more attention to individual-oriented self-realization. Compassionate mindfulness has a greater positive moderating effect on the mental health of women aged 30–39 and those who are highly educated. Compassionate mindfulness has a more positive moderating effect on the psychological needs of members of Generation Y who were born more recently. During the COVID-19 pandemic, providing compassionate mindfulness has a significant positive effect on the prevention of mental disorders of Generation Y in Taiwan.
The Psychological Experience of COVID-19 Vaccination and Its Impact on the Willingness to Receive Booster Vaccines among the Chinese Population: Evidence from a National Cross-Sectional Study
Authors: Miao Yudong , Li Yi , Zhang Wanliang , Wu Jian , Gu Jianqin , Wang Meiyun , Wei Wei , Ye Beizhu , Miao Chengyuan , Tarimo Clifford Silver , Dong Wenyong
Published: April 2022

The purpose of this study was to assess the psychological experience of COVID-19 basic vaccination, the willingness to receive booster vaccines, and to determine their relationships among Chinese people. Between 6 August 2021 and 9 August 2021, a research firm performed a national cross-sectional online survey among Chinese individuals (aged over 18), using the snowball sampling approach, with 26,755 participants. Factor analysis and binary logistic regression were used to evaluate the existing associations. The overall COVID-19 vaccination psychological experience score of the participants was 25.83 (25.78~25.89; scores ranged from 7–35). A total of 93.83% (95%CI = 93.54~94.12) of respondents indicated a willingness to receive booster vaccines. After classifying psychological experiences associated with COVID-19 vaccination into positive and negative experiences and adjusting for confounding factors, for the former, the willingness to receive booster vaccines for participants with the highest scores of 13–15 was 3.933 times higher (OR = 3.933, 95%CI = 3.176~4.871) than participants who obtained scores of 3–9, and for the latter, the willingness to receive booster vaccines for participants with the highest scores of 19–20 was 8.871 times higher (OR = 8.871, 95%CI = 6.240~12.612) than participants who obtained scores of 4–13. Our study suggests that a good psychological experience with vaccination is positively associated with an increased willingness to receive booster vaccines.
Prevalence and Determinants of Hoarseness in School-Aged Children
Authors: Alrahim Ahmed , Alshaibani Askar K. , Algarni Saad , Alsaied Abdulmalik , Alghamdi Amal A. , Alsharhan Salma , Al-Bar Mohammad
Published: April 2022

Hoarseness in school-aged children may affect their educational achievement and interfere with their communication and social skills development. The global prevalence of hoarseness in school-aged children ranges between 6% and 23%. To the best of our knowledge, there is a scarcity of studies describing the prevalence or determinates of hoarseness in Saudi school-aged children. Our aim was to measure the prevalence of hoarseness among school-aged children and to identify its determinants. A cross-sectional questionnaire-based survey was used that included randomly selected primary and early childhood schools from private and governmental sectors in Saudi Arabia. The data were collected using a questionnaire which was self-completed by the children’s parents and covered the following aspects: sociodemographic features, health and its related comorbidities about children and their families, attendance and performance in school, child’s voice tone, past history of frequent crying during infancy, history of letter pronunciation problems and stuttering, the Reflux Symptom Index (RSI) and the Children’s Voice Handicap Index-10 for parents (CVHI-10-P). Determinants of hoarseness were investigated using the SPSS software (version 20). The mean age of the study children (n = 428) was 9.05 years (SD = 2.15), and 69.40% of them were male. The rate of hoarseness in the participants was 7.5%. Hoarseness was significantly common in children with a history of excessive infancy crying (p = 0.006), letter pronunciation issues (especially ‘R’ and ‘S’; p = 0.003), and stuttering (p = 0.004) and in those with a previous history of hoarseness (p = 0.023). In addition, having the symptoms of gastrointestinal reflux increased the risk of hoarseness by four times (OR = 4.77, 95% CI = 2.171, 10.51). In summary, hoarseness in children may be dangerously underestimated, as it may reflect the presence of speech problems, in addition to the presence of laryngopharyngeal reflux (LPR). Hoarseness was assumed on the basis of parental complaints. Therefore, further research with diagnoses based on a clinical assessment is needed to understand the magnitude of the hoarseness problem and its consequences in children.
The Dynamic Typology in the Development Process of Credit Union Movements
Authors: Kang Chien-Min , Wang Ming-Chieh , Lin Lin
Published: April 2022

The aim of this paper is to find a dynamic framework of analysis of credit union movements by grouping credit unions into different category types. Within the heterogeneous reality of the worldwide credit union movement, the typology provides a clearer understanding of the dynamics of change and development. We use panel-ordered logistic regression to find the key covariates of influence when analyzing the original typology to add further explanation of the development of credit union movements. By using transnational research, we revisit each of the three categories of the original typology to re-evaluate and expand upon the relevance of this particular model. We also include the elements of economy, society, education, and culture in other countries in this research. Our findings suggest that the stage of development of the credit union movement depends on the variables of asset scale, financial crisis, legislative framework, economy, society, and culture of the country. In addition, they indicate that the penetration rate of the credit union movement depends on the asset scale, loan ratio, credit union growth, financial crisis, economy, society, education, and culture of the country. This lends support to the recognition of the diversity of the credit unions’ development.
GeoSDVA: A Semi-Supervised Dirichlet Variational Autoencoder Model for Transportation Mode Identification
Authors: Zhang Xiaoxi , Gao Yuan , Wang Xin , Feng Jun , Shi Yan
Published: April 2022

Inferring the transportation modes of travelers is an essential part of intelligent transportation systems. With the development of mobile services, it is easy to effectively obtain massive location readings of travelers with GPS-enabled smart devices, such as smartphones. These readings make understanding human activities very convenient. Therefore, how to automatically infer transportation modes from these massive readings has come into the spotlight. The existing methods for transportation mode identification are usually based on supervised learning. However, the raw GPS readings do not contain any labels, and it is expensive and time-consuming to annotate sufficient samples for training supervised learning-based models. In addition, not enough attention is paid to the problem that GPS readings collected in urban areas are affected by surrounding geographic information (e.g., the level of road transportation or the distribution of stations). To solve this problem, a geographic information-fused semi-supervised method based on a Dirichlet variational autoencoder, named GeoSDVA, is proposed in this paper for transportation mode identification. GeoSDVA first fuses the motion features of the GPS trajectories with the nearby geographic information. Then, both labeled and unlabeled trajectories are used to train the semi-supervised model based on the Dirichlet variational autoencoder architecture for transportation mode identification. Experiments on three real GPS trajectory datasets demonstrate that GeoSDVA can train an excellent transportation mode identification model with only a few labeled trajectories.
Direct T-2 Toxicity on Human Skin—Fibroblast Hs68 Cell Line—In Vitro Study
Authors: Janik-Karpinska Edyta , Ceremuga Michal , Wieckowska Magdalena , Szyposzynska Monika , Niemcewicz Marcin , Synowiec Ewelina , Sliwinski Tomasz , Bijak Michal
Published: April 2022

T-2 toxin is produced by different Fusarium species, and it can infect crops such as wheat, barley, and corn. It is known that the T-2 toxin induces various forms of toxicity such as hepatotoxicity, nephrotoxicity, immunotoxicity, and neurotoxicity. In addition, T-2 toxin possesses a strong dermal irritation effect and can be absorbed even through intact skin. As a dermal irritant agent, it is estimated to be 400 times more toxic than sulfur mustard. Toxic effects can include redness, blistering, and necrosis, but the molecular mechanism of these effects still remains unknown. This in vitro study focused on the direct toxicity of T-2 toxin on human skin—fibroblast Hs68 cell line. As a result, the level of toxicity of T-2 toxin and its cytotoxic mechanism of action was determined. In cytotoxicity assays, the dose and time-dependent cytotoxic effect of T-2 on a cell line was observed. Bioluminometry results showed that relative levels of ATP in treated cells were decreased. Further analysis of the toxin’s impact on the induction of apoptosis and necrosis processes showed the significant predominance of PI-stained cells, lack of caspase 3/7 activity, and increased concentration of released Human Cytokeratin 18 in treated cells, which indicates the necrosis process. In conclusion, the results of an in vitro human skin fibroblast model revealed for the first time that the T-2 toxin induces necrosis as a toxicity effect. These results provide new insight into the toxic T-2 mechanism on the skin.
Early Growth Response 1 Strengthens Pol-III-Directed Transcription and Transformed Cell Proliferation by Controlling PTEN/AKT Signalling Activity
Authors: Wu Zhongyu , Huang Liyun , Zhao Shasha , Wang Juan , Zhang Cheng , Song Xiaoye , Chen Qiyue , Du Jiannan , Yu Deen , Sun Xiaomeng , Zhang Yue , Deng Wensheng , Zhang Shihua , Deng Huan
Published: April 2022

RNA polymerase III (Pol III) products play essential roles in ribosome assembly, protein synthesis, and cell survival. Deregulation of Pol-III-directed transcription is closely associated with tumorigenesis. However, the regulatory pathways or factors controlling Pol-III-directed transcription remain to be investigated. In this study, we identified a novel role of EGR1 in Pol-III-directed transcription. We found that Filamin A (FLNA) silencing stimulated EGR1 expression at both RNA and protein levels. EGR1 expression positively correlated with Pol III product levels and cell proliferation activity. Mechanistically, EGR1 downregulation dampened the occupancies of Pol III transcription machinery factors at the loci of Pol III target genes. Alteration of EGR1 expression did not affect the expression of p53, c-MYC, and Pol III general transcription factors. Instead, EGR1 activated RhoA expression and inhibited PTEN expression in several transformed cell lines. We found that PTEN silencing, rather than RhoA overexpression, could reverse the inhibition of Pol-III-dependent transcription and cell proliferation caused by EGR1 downregulation. EGR1 could positively regulate AKT phosphorylation levels and is required for the inhibition of Pol-III-directed transcription mediated by FLNA. The findings from this study indicate that EGR1 can promote Pol-III-directed transcription and cell proliferation by controlling the PTEN/AKT signalling pathway.
Neurotrophic Factors in Experimental Cerebral Acanthamoebiasis
Authors: Łanocha-Arendarczyk Natalia , Kot Karolina , Baranowska-Bosiacka Irena , Kapczuk Patrycja , Łanocha Aleksandra , Kosik-Bogacka Danuta Izabela
Published: April 2022

To date, no studies have addressed the role of neurotrophins (NTs) in Acanthamoeba spp. infections in the brain. Thus, to clarify the role of NTs in the cerebral cortex and hippocampus during experimental acanthamoebiasis in relation to the host immune status, the purpose of this study was to determine whether Acanthamoeba spp. may affect the concentration of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) in brain structures. Our results suggest that at the beginning of infection in immunocompetent hosts, BDNF and NT-3 may reflect an endogenous attempt at neuroprotection against Acanthamoeba spp. infection. We also observed a pro-inflammatory effect of NGF during acanthamoebiasis in immunosuppressed hosts. This may provide important information for understanding the development of cerebral acanthamoebiasis related to the immunological status of the host. However, the pathogenesis of brain acanthamoebiasis is still poorly understood and documented and, therefore, requires further research.
Ab Initio Study of the Interaction of a Graphene Surface Decorated with a Metal-Doped C30 with Carbon Monoxide, Carbon Dioxide, Methane, and Ozone
Authors: Canales Mónica , Ramírez-de-Arellano Juan Manuel , Arellano Juan Salvador , Magaña Luis Fernando
Published: April 2022

Using DFT simulations, we studied the interaction of a semifullerene C30 and a defected graphene layer. We obtained the C30 chemisorbs on the surface. We also found the adsorbed C30 chemisorbs, Li, Ti, or Pt, on its concave part. Thus, the resulting system (C30-graphene) is a graphene layer decorated with a metal-doped C30. The adsorption of the molecules depends on the shape of the base of the semifullerene and the dopant metal. The CO molecule adsorbed without dissociation in all cases. When the bottom is a pentagon, the adsorption occurs only with Ti as the dopant. It also adsorbs for a hexagon as the bottom with Pt as the dopant. The carbon dioxide molecule adsorbs in the two cases of base shape but only when lithium is the dopant. The adsorption occurs without dissociation. The ozone molecule adsorbs on both surfaces. When Ti or Pt are dopants, we found that the O3 molecule always dissociates into an oxygen molecule and an oxygen atom. When Li is the dopant, the O3 molecule adsorbs without dissociation. Methane did not adsorb in any case. Calculating the recovery time at 300 K, we found that the system may be a sensor in several instances.
Moving Average-Based Multitasking In Silico Classification Modeling: Where Do We Stand and What Is Next?
Authors: Halder Amit Kumar , Moura Ana S. , Cordeiro Maria Natália D. S.
Published: April 2022

Conventional in silico modeling is often viewed as ‘one-target’ or ‘single-task’ computer-aided modeling since it mainly relies on forecasting an endpoint of interest from similar input data. Multitasking or multitarget in silico modeling, in contrast, embraces a set of computational techniques that efficiently integrate multiple types of input data for setting up unique in silico models able to predict the outcome(s) relating to various experimental and/or theoretical conditions. The latter, specifically, based upon the Box–Jenkins moving average approach, has been applied in the last decade to several research fields including drug and materials design, environmental sciences, and nanotechnology. The present review discusses the current status of multitasking computer-aided modeling efforts, meanwhile describing both the existing challenges and future opportunities of its underlying techniques. Some important applications are also discussed to exemplify the ability of multitasking modeling in deriving holistic and reliable in silico classification-based models as well as in designing new chemical entities, either through fragment-based design or virtual screening. Focus will also be given to some software recently developed to automate and accelerate such types of modeling. Overall, this review may serve as a guideline for researchers to grasp the scope of multitasking computer-aided modeling as a promising in silico tool.
Antigens and Antibodies of the Antiphospholipid Syndrome as New Allies in the Pathogenesis of COVID-19 Coagulopathy
Authors: Serrano Manuel , Espinosa Gerard , Serrano Antonio , Cervera Ricard
Published: April 2022

High prevalence of both criteria and extra-criteria antiphospholipid antibodies (aPL) has been reported in COVID-19 patients. However, the differences in aPL prevalence decreased when an age-matched control group was included. The association of aPL with thrombotic events in COVID-19 is very heterogeneous. This could be influenced by the fact that most of the studies carried out were conducted on small populations enriched with elderly patients in which aPL was measured only at a single point and they were performed with non-standardized assays. The few studies that confirmed aPL in a second measurement showed that aPL levels hardly changed, with the exception of the lupus anticoagulant that commonly reduced. COVID-19 coagulopathy is an aPL-independent phenomenon closely associated with the onset of the disease. Thrombosis occurs later in patients with aPL presence, which is likely an additional prothrombotic factor. B2-glycoprotein deficiency (mainly aPL antigen caused both by low production and consumption) is very common during the SARS-CoV2 infection and has been associated with a greater predisposition to COVID-19 complications. This could be a new prothrombotic mechanism that may be caused by the blockage of its physiological functions, the anticoagulant state being the most important.
FTO Alleviates CdCl2-Induced Apoptosis and Oxidative Stress via the AKT/Nrf2 Pathway in Bovine Granulosa Cells
Authors: Ding He , Li Zhiqiang , Li Xin , Yang Xiaorui , Zhao Jing , Guo Jing , Lu Wenfa , Liu Hongyu , Wang Jun
Published: April 2022

Cadmium (Cd) is a common environmental heavy metal contaminant of reproduction toxicity. Cd accumulation in animals leads to the damage of granulosa cells. However, its mechanism needs to be elucidated. This research found that treating granulosa cells with Cd resulted in reduced cell viability. The flow cytometry results showed that Cd increased the degree of apoptosis and level of superoxide anion (O2−) in granulosa cells. Further analysis showed that Cd treatment resulted in reduced expression levels of nuclear factor erythroid 2-related factor-2 (Nrf2), superoxide dismutase (SOD), catalase (CAT) and NAD(P)H: quinone oxidoreductase 1 (NQO1), and an increased expression level of malondialdehyde (MDA); the expression levels of Bcl-2 associated X (Bax) and caspase-3 increased, whereas that of B-cell lymphoma 2 (Bcl-2) decreased. Changes in m6A methylation-related enzymes were noted with Cd-induced damage to granulosa cells. The results of transcriptome and MeRIP sequencing revealed that the AKT pathway participated in Cd-induced damage in granulosa cells, and the MAX network transcriptional repressor (MNT) may be a potential target gene of fat mass and obesity-associated protein (FTO). FTO and YTH domain family member 2 (YTHDF2) regulated MNT expression through m6A modification. FTO overexpression alleviated Cd-induced apoptosis and oxidative stress through the activation of the AKT/Nrf2 pathway; this process could be reversed using siMNT. Overall, these findings associated m6A with Cd-induced damage to granulosa cells and provided insights into Cd-induced granulosa cell cytotoxicity from a new perspective centered on m6A modification.