bluuebunny/crossref_metadata_2025 huggingface dataset:
Easy Handling Preparation of Cubic Sulfur in Aqueous Extract of Sapindus rarak rinds
Authors: Banon Charles , Nesbah Nesbah , Trihadi Bambang , Falahudin Aswin , Yudha S Salprima
Published: December 2022

The aqueous extract of Sapindus rarak (S. rarak) was produced by heating its rinds in demineralized water at 80 °C. The main experiment was conducted at room temperature by mixing a solution of sodium thiosulfate with the extract obtained previously. After adding dilute hydrochloric acid (10%), fine granules gradually formed in the solution and precipitated when the reaction was stopped and allowed to stand for 24 h. The analysis results showed the consistency of the X-ray diffraction (XRD) peak of the obtained material with sulfur standards. When looked at the surface using scanning electron microscopy (SEM), sulfur was found to be cube-shaped. The formation of cuboidal elemental sulfur possibly occurs due to the covering of thiosulfate ions and elemental sulfur during and after the reaction. Organic compounds were found covering sulfur through elemental and functional group analyses using energy-dispersive X-ray (EDX) and Fourier-transform infrared (FTIR) spectroscopy, respectively
Potential Indonesian Natural Compound as antiviral for COVID-19 targeting the RdRp: In silico Study
Authors: Muscifa Zahra Silmi , Sumaryada Tony , Ambarsari Laksmi , Wahyudi Setyanto Tri
Published: December 2022

Research related to SARS-CoV-2 drugs is still ongoing. In this initial research, we perform a computational approach on SARS-CoV-2 inhibitors. RNA-dependent RNA polymerase (RdRp) is one of the functional proteins in SARS-CoV-2 that can be a target for drug development, which has an essential function in the viral replication process synthesizing the RNA genome of the virus. This study used the RdRp-Remdesivir complex structure from RCSB with ID PDB 7BV2, with a resolution of 2.5 Å. Currently, Remdesivir is under the clinical trial phase as a Covid-19 drug. In this study, we tested a thousand natural Indonesian compounds used as SARS-CoV-2 RdRp inhibitors obtained from the Indonesian natural compounds database (HerbalDB). The first stage of this computational analysis was pharmacophore modeling structure-based drug design. The natural compounds were analyzed based on their steric and electronic similarities to Remdesivir. A molecular docking simulation was then performed to obtain binding energy and bond stability to produce natural compounds that can inhibit RdRp SARS-CoV-2. The final stage was the molecular dynamics simulation that explored the conformational space of natural compounds and proteins. The ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) test was carried out on the five best compounds to obtain these natural compounds' computational pharmacology and pharmacokinetics. The simulation identified Sotetsuflavone (CID: 5494868) from Cycas revoluta, Grossamide (CID: 5322012) from Cannabis sativa, and 6-Hydroxyluteolin-6,7-disulfate (CID: 13845917) from Lippia nodiflora are the best compounds that can inhibit RdRp SARS-CoV-2. These potential compounds can then be tested in-vitro and in-vivo in the future.
Concurrently wireless sensor network using microcontroller for home monitoring against fire
Authors: Prasetyananda Putu Wiweka , Sudiro Sunny Arief , Wardijono Bheta Agus
Published: December 2022

This paper proposes a prototype fire monitoring system with Wireless Sensor Network (WSN) in order to detect fires in several places at once and facilitate the placement of the detection.  WSN is a technology where several sensors work together to establish communication over a wireless network. This prototype fire monitoring system can be monitored through the website in real time and the detection data is stored on the cloud server. This prototype fire monitoring system uses 4 sensor nodes, each of which is placed in several places to detect fires and sends the detection results to the master node. The master node receives and processes the data sent by the sensor node to generate 16 different conditions concurrently, in the event of a fire it will send a telegram message and the condition data to the cloud server. Several attempts to send data from the sensor node to the master node were completely successful and sending data from the master node to the cloud server as well as sending notification messages have been sent properly.
A New Set-up of Vanishing Antibodies: A Biennial Follow-up of Five Different Clients’ Humoral Responses Against Sars-Cov-2 After Systemic Vaccination in an Oncology Hospital in Poland
Authors: Kosiorek Piotr , Stróż Samuel , Hryniewicz Anna , Milewski Robert , Bartoszewicz Klaudia , Stasiak-Barmuta Anna
Published: December 2022

The humoral response of the COVID-19 vaccine varies from person to person. It largely depends on prior SARS-CoV-2 infection, obtaining an adequate immune response, and leaving a trace of changing antibody concentration over time. We retrospectively analyzed five clinical cases from selected patients and employees of the oncology hospital. All mild COVID-19 convalescents received the BNT162b2-Comirnaty mRNA vaccine three or four times. The levels of SARS-CoV-2 IgM- and IgG-specific antibodies, as well as S-RBD antibodies, were analyzed for two years. The concentration of antibodies was assessed in the laboratory using the chemiluminescent immunoassay CLIA, MAGLUMI. Results: (1) Active autoimmune disease stabilized the level of IgG-specific antibodies after systemic mRNA vaccination for at least six months. (2) Post-vaccination IgG and S-RBD levels decreased when vaccination was performed within three months of onset. (3) The booster dose administered only increased the S-RBD antibody levels. Declining IgG-specific antibodies were observed. (4) The S-RBD IgG levels were not correlated with the SARS-CoV-2 IgG levels in the vaccinated convalescents. (5) Subsequent reinfection with SARS-CoV-2 after vaccination three times released a more significant specific antibody response. Based on the collected data, we suggest that monitoring S-RBD antibodies is sensitive but not equivalent to a specific humoral response for SARS-CoV-2 IgG. We suggested that administering at least three doses of the mRNA vaccine should serve as the basis for immunization. The three-month interval may be the best alternative to an immunization schedule for non-immunocompromised people.
Indexing Inefficacy of Efforts to Stop Escalation of COVID Mortality
Authors: Shanmugam Ram , Fulton Lawrence , Betancourt Jose , Pacheco Gerardo J
Published: December 2022

Background. COVID-19 efforts were often ineffective in controlling the spread of the pandemic. Identifying ineffective controls during a pandemic is thus vital. Method. Utilizing publicly available data on COVID deaths in the counties of US states, we create an index to capture and interpret ineffectiveness in the efforts to reduce the spread of the pandemic in US counties. This index is based on the Intervened Poisson Distribution (IPD) introduced originally by Shanmugam. Motivation for the research idea occurred while we noticed the data dispersion of the COVID deaths is smaller than the average only in some counties. Under-dispersed data is common in statistical modeling. A novel approach we adapted in this article includes the estimation of an intervention parameter estimated through iterative non-linear optimization. Results. Twenty-five counties in California, Idaho, Minnesota, Mississippi, Montana, Nebraska, North Carolina, North Dakota, Texas, and Utah were found to be ineffective in controlling for fatalities based on the expected probability distribution. A review of the policies enacted in these areas would provide insight into ineffective prevention efforts, and some of these issues are documented in current literature. Conclusion. The IPD index an innovate way to document efficacy of interventions during pandemics. The IPD may identify ineffective efforts prior to statistical models intended to evaluate efficacy of efforts.
A Novel Data-Driven Fuzzy for Accurate Coagulant Dosage in Drinking Water Treatment
Authors: Bressane Adriano , Goulart Ana Paula Garcia , Gomes Isadora Gurjon , Loureiro Anna Isabel Silva , Negri Rogério Galante , Braga Moruzzi Rodrigo , Reis Adriano Gonçalves dos , Formiga Jorge K. S. , Melo Carrie Peres , Silva Gustavo H. R.
Published: December 2022

Coagulation is the most sensitive step in drinking water treatment. Underdosing may not yield the required water quality, whereas overdosing may result in higher costs and excess sludge. Traditionally, the coagulant dosage is set based on bath experiments performed manually. Therefore, this test does not allow real-time dosing control, and its accuracy is subject to operator experience. Alternatively, solutions based on machine-learning (ML) have been evaluated as a computer-aided alternative. Despite these advances, there is open debate on the most suitable ML method applied to the coagulation process, capable of the most highly accurate prediction. This study addresses this gap, where a comparative analysis between ML methods was performed. As a research hypothesis, a novel data-driven fuzzy inference system (FIS) should provide the best performance due to its ability to deal with uncertainties inherent to complex processes. Although ML methods have been widely investigated, only a few studies report hybrid neuro-fuzzy systems applied to coagulation. Thus, to the best of our knowledge, this is the first study thus far to address the accuracy of this novel data-driven FIS for such application. The novel FIS provided the smallest error (0.86), indicating a promising alternative tool for real-time and highly accurate coagulant dosing in drinking water treatment.
Soil Organic Matter and Nutrient Levels in Outdoor Runs in Organic Laying Farms
Authors: Zoli Michele , Mantovi Paolo , Ferrari Paolo , Ferrari Lorenzo , Ferrante Valentina
Published: December 2022

To evaluate the nutrient load due to the grazing of laying hens in outdoor runs, soil characteristics were monitored in three Italian organic farms. For each farm, soil samples were taken from three increasing distances from the hen house and two depths and different chemical parameters were evaluated. The comparison among the results from the different distances shows that N-NO3 and Olsen P are the most affected parameters by hen faeces: both present high values with a statistically significant difference in the area close to the poultry house and for the most superficial layer. Even TKN and TOC show significant differences between the concentrations of the first layer (more concentrated) and those of the second layer (less concentrated). In general, the surface soil layer closest to the chicken house is the portion of the outdoor run most affected by chicken droppings and represents the most critical point in terms of potential environmental impact. Therefore, it is necessary to intensify the management of the outdoor run with tools that can facilitate the grazing of animals and with vegetation that can absorb nutrients by limiting leaching and run-off.
Residential Building Damages Related to the Geological Bedrock Variations During Mww 5.6 Earthquake in Cianjur, West Java, on November 21, 2022
Authors: Wibowo Andri
Published: December 2022

Recently on the November 4, 2022 at 03:00 PM, a Mww 5.6 earthquake struck Cianjur district of West Java province, Indonesia. To our best knowledge, an earthquake will cause a massive damage to the manmade infrastructure and this responsible for a massive casualty. A building damage is affected by various determinant factors. One of important factors is the geological bedrock variations. Here this research aiming to elaborate the associations of geological bedrocks in Cianjur district with residential building damages caused by recent earthquakes. The results confirm that majority of building damages account for 54.67% of the total building was associated with quaternary volcanic bedrocks. The second massive building damages were observed for Andesitic Dacitic volcanic rocks accounted for 32.07%. To conclude, elaborations of geological bedrock-building damage will contribute to the better planning in the future that can reduce the damages in the urban settings.
Hubungan Perceived Stigma dengan Tingkat Depresi pada Orang dengan HIV/AIDS di Kelompok Dukungan Sebaya Solo Plus Kota Surakarta
Authors: Johanna Elisha  , Istar Yuliadi  , Ipop Sjarifah
Published: December 2022

Pendahuluan: Orang dengan HIV/AIDS (ODHA) sering merasakan perceived stigma yang memiliki dampak terhadap kesehatan mental mereka. Penelitian ini bertujuan untuk mengetahui dan menganalisis apakah terdapat hubungan perceived stigma dengan tingkat depresi pada ODHA di Kelompok Dukungan Sebaya (KDS) Solo Plus Kota Surakarta.
Metode: Penelitian ini merupakan penelitian observasional analitik dengan desain penelitian cross-sectional yang dilakukan pada 61 orang dengan HIV/AIDS di KDS Solo Plus Kota Surakarta yang dipilih secara simple random sampling. Variabel bebas dalam penelitian ini adalah perceived stigma dengan variabel terikat tingkat  depresi. Teknik analisis yang digunakan adalah uji korelasi Pearson.
Hasil: Analisis bivariat hubungan perceived stigma dengan tingkat depresi memiliki nilai p=0,000 dan nilai r=0,715.
Kesimpulan: Terdapat hubungan yang positif antara perceived stigma dengan tingkat depresi pada orang dengan HIV/AIDS di KDS Solo Plus Kota Surakarta.
Configuración de un territorio urbano moderno en la frontera norte. Ciudad Juárez 1880-1920
Authors: Montiel Mendez Yolanda Elena
Published: December 2022

Ciudad Juárez es hoy en día considerada una metrópoli, pero a finales del siglo XIX era apenas una villa y a principios del XX fue que comenzó su crecimiento y “modernización”. En esta investigación se pretende explicar la evolución de la configuración física de este territorio urbano fronterizo durante el periodo 1880-1920, por medio de la investigación documental e historiográfica, además de analizar mapas de le .poca, a trav.s de diversas variables que ayudan a poder distinguir las áreas de crecimiento. Los resultados observados explican cómo estas influyeron en su peculiar configuración.
Sex, Pain & Endometriosis: The development of a patient-centred e-health resource for those affected by endometriosis-associated dyspareunia
Authors: Parmar Gurkiran , Howard A. Fuchsia , Noga Heather , Tannock Leah , Abdulai Abdul-Fatawu , Allaire Catherine , Lett Sarah , Sutherland Jessica , de Arbina Edurne Lopez , Hummelshoj Lone , Bridge-Cook Philippa , Yong Paul J.
Published: December 2022

Background
We recognized a paucity of accessible, evidence-based, empowering patient-centred resources for those with endometriosis-associated dyspareunia. We aimed to develop a patient-centred educational website for those affected by endometriosis-associated dyspareunia.
Methods
To develop a functional and meaningful website for endometriosis-associated dyspareunia, we utilized a Knowledge to Action framework, supplemented with a patient-centred research design and technology-enabled knowledge translation. Our patient partners influenced the direction and scope of the project, provided critical feedback throughout the development process, and approved website revisions prior to launch. The website was developed in five phases; 1) needs assessment interviews and focus groups with key stakeholders, 2) landscape analysis of pre-existing websites, 3) development, 4) usability testing, and qualitative interviews, and 5) revisions and launch.
Results
Phase 1 and 2 emphasized a need for comprehensive yet plain language explanations of pain mechanisms and strategies for pain management. Rigorous consultation with key stakeholders informed the creation of the preliminary website, which was tested in phase 4. User testing identified five main categories of usability problems, of which the majority were considered minor. Qualitative interviews identified users’ overall impressions of the preliminary website, including that the website could help people understand their pain and describe their pain to partners and healthcare providers, as well as feel empowered to seek healthcare and validated in their experiences. User suggestions, combined with usability testing informed revisions.
Conclusion
We developed an educational website for endometriosis-associated painful sex where people can find evidence-based etiologies for pain, pain management options, and actionable resources. Based on the data collected through qualitative interviews with patients, this website has the potential to empower people to seek health care. The strength of the website development approach used was the inclusion of qualitative user insights in addition to the commonly completed user tests. The patient interviews provided insights into the potential impact of the website and thus, ensured that we not only created a functional website that meets end users’ needs, but a website that is also meaningful to those affected by this condition.
A Case Study on Impact of Electronic Health Records System (EHRS) on Healthcare Quality at Asamankese Government Hospital
Authors: Antwi Frimpong Matthew
Published: December 2022

The main objective of this study was to explore the impact of EHRs on healthcare quality at the Asamankese Government Hospital. The research used three objectives thus, explore the use, behavioral intent and the impact of EHRs on healthcare quality among clients and staffs of the facility. This is qualitative research with a case study design with data gathered through interviews from five clients and eight staffs. The study proved all departments of the Hospital were fully using EHRs with all respondents willing to continue its use. The key findings of the study were, firstly, improved storage and quality of medical records and easy retrieval of records by healthcare practitioners. EHRs reduces administrative and operational cost. Thirdly, EHRs improves client safety, staff productivity and efficiency healthcare delivery. Furthermore, it reduces clients’ waiting time, waste of resources and helping track expiry dates of medications and improves health outcomes. Finally, EHRs improves triaging and responsive care and the personal healthcare of clients thereby improving clients’ satisfaction on services rendered at the facility. The EHRs however faced challenges such us unstable internet network, electricity instability, inadequate training of staffs, etc. The study concludes EHRs has improved healthcare quality at the Asamankese Government Hospital.
13C-isotope-editing of nanoscale infrared images reveals the action of an inhibi­tory peptide against amyloid-β aggregation
Authors: Paul Suman , Berntsson Elina , Mörman Cecilia , Jeništová Adéla , Jarvet Jüri , Gräslund Astrid , Wärmländer Sebastian , Barth Andreas
Published: December 2022

Interactions between molecules are fundamental in biology. They occur also between amyloidogenic polypeptides that are associated with different amyloid diseases, which makes it important to study their interactions. However, addressing such research questions with imaging techniques is hindered by the problem to distinguish different polypeptides without adding artificial probes for detection. Here we present a new method for this purpose. We show that 13C-labeling can be used to distinguish peptides in nanoscale images of their infrared absorption, even when they have similar secondary structure. We studied different aggregation states of the amyloid-β peptide (Aβ) and its interaction with an inhibitory cell-penetrating peptide (NCAM1-PrP) using scattering-type scanning near field microscopy. Labeled and unlabeled peptides could be discriminated by comparing images of the optical phase taken at wavenumbers characteristic for either the labeled or the unlabeled peptide. NCAM1-PrP seems to be able to dissolve or coat existing Aβ fibrils because "naked" Aβ fibrils are not detected after mixing.
Clustering of lifestyle behaviors and analysis of their associations with MAFLD: a cross- sectional study of 196515 in China
Authors: Zhou Bingqian , Gong Ni , He Qingnan , Huang Xinjuan , Zhu Jingchi , Zhang Lijun , Huang Yanyan , Tan Xinyun , Xia Yuanqin , Zheng Yu , Shi Qiuling , Qin Chunxiang
Published: December 2022

Introduction

The aggregation of lifestyle behaviors and their association with metabolic associated fatty liver disease (MAFLD) remains unclear. We identified lifestyle patterns and investigated their association with MAFLD in a sample of Chinese adults who underwent annual physical examinations.

Methods

Annual physical examination data of Chinese adults from January 2016 to December 2020 was used in this study. We created a scoring system for lifestyle items combining statistical method (Multivariate analysis of variance) and clinical expertise’s opinion (Delphi method). Subsequently, principal components analysis and two-step cluster analysis were implemented to derive lifestyle patterns of men and women. Binary logistic regression analysis was used to explore the prevalence risk of MAFLD among lifestyle patterns stratified by gender.

Results

A total of 196,515 subjects were included in the analysis. Based on the defined lifestyle scoring system, nine and four lifestyle patterns were identified for men and women, respectively, which included “healthy or unhealthy” patterns and mixed patterns containing a combination of healthy and risky lifestyle behaviors. This study showed that subjects with an unhealthy or mixed pattern had a differentially higher risk of developing MAFLD than subjects with a relatively healthy pattern, especially among men.

Conclusions

Clusters of unfavorable behaviors are more prominent in men when compared to women. Lifestyle patterns, as the important factors influencing the development of MAFLD, show significant gender differences in the risk of MAFLD. There is a strong need for future research to develop targeted MAFLD interventions based on the identified behavioral clusters by gender stratification.
Antenatal N-acetylcysteine antioxidant therapy relieves lung oxidative stress and related perinatal lethality in HRas/NRas double-null mutant mice
Authors: Fuentes-Mateos Rocío , García-Navas Rósula , Calzada Nuria , Santos Eugenio , Fernández-Medarde Alberto
Published: December 2022

We reported previously that concomitant HRas/NRas ablation causes very high rates of mortality in mice at birth (P0) due to severe respiratory distress and defective lung maturation which was linked to significant ceramide accumulation and delayed alveolar differentiation in this respiratory organ. Antenatal treatment with dexamethasone (DEX), a glucocorticoid known to boost lung maturation, reverted the lung immaturity of our HRas/NRas double-null mutant (DKO) animals but failed to rescue their elevated perinatal lethality. Our search for mechanistic clues to these deleterious phenotypes uncovered significantly increased levels of intracellular oxidative stress, altered mitochondrial electron transport and respiratory function (Oxygen Consumption Rate, OCR), and elevated neutrophil infiltration in the lungs of our HRas/NRas-DKO mice. We also evaluated the functional effects of antenatal antioxidant therapy using N-acetylcysteine (NAC) on postnatal survival and the defective lung phenotypes of our double-KO mice. In contrast to DEX treatment, we observed that antenatal NAC treatment resulted in normal lung development and the survival to adulthood of more than 50% of the newborn HRas/NRas-DKO pups. We also observed that the redox imbalance of our double-KO newborn pups was fully corrected upon NAC antioxidant treatment, but only partially by dexamethasone, indicating that the lung oxidative stress played a major role in the lethality of the DKO mice. Our observations provide a direct mechanistic link between the absence of HRas and NRas and the control of lung intracellular oxidative stress, the perinatal maturation of specific pulmonary cellular lineages, and the subsequent survival of the newborn pups.
A SafeML Extension for a Unified Risk Assessment to Diverse Service Robots
Authors: Miyoshi Takao , Nakabo Yoshihiro , Fukui Hidetoshi , Yashiro Makoto , Miyazawa Iko , Sakamoto Takeshi , Ando Noriaki , Kuga Toru , Kitamura Atsushi , Ohara Kenichi , Kimura Tetsuya
Published: December 2022

Risk assessment is one of the important processes in the social implementation of robots. In risk assessment and safety design of systems with various stakeholders, modeling and visualization of related elements are important not only for designers but also for users. We examined the use of SafeML in the process and proposed its extension for the purpose for missing elements. Additionally, we submitted this paper after we received recommendations on content presented at “JSME Conference on Robotics and Mechatronics 2022”and added considerations.
Ultra-Thin Zr-MOF/PVA/Melamine Composites with Remarkable Sound Attenuation Effects
Authors: Lan Yi-Chen , Kamal Surabhi , Lin Chih-Chien , Liu Yen-Hsiang , Lu Kuang-Lieh
Published: December 2022

The rising awareness of the critical nature of noise pollution is driving more efforts toward the development of new sound-absorbing materials for use in a variety of engineering applications. Nevertheless, the efficiency of noise control and the thickness of these materials continue to be a formidable barrier to implementation. To address this issue, we report herein on the successfully development of an innovative ultrathin Zr-MOF/PVA/M composite with exceptional sound attenuation characteristics. This Zr-MOF composite sound absorber was fabricated by integrating a UiO-66/PVA solution into a melamine foam. The as-prepared porous structure, with a thickness of 5 mm, exhibited a 2.4 time attenuation of sound in the high frequency range compared to that for the pristine melamine foam, which is crucial for its applications. The inclusion of a PVA solution aided in the development of a uniform distribution, while the embedded Zr-MOFs acted as microscale Helmholtz resonators, significantly improving sound attenuation. In addition, the formation of Zr-MOF/PVA films enclosed in the open cells of melamine, thus creating a semi-open porous structure that reflects the sound within the framework, effectively dampening the sound energy. These favorable properties result in hybrid sound-absorbing MOFs that have potential for use in noise reduction in the fields of construction, electrical and mechanical devices.
An integrative bioinformatics investigation and experimental validation of Chromobox family in Diffuse large B-cell lymphoma
Authors: Zhou Fenling , Chen Lu , Lu Peng , Cao Yuli , Deng Cuilan , Liu Gexiu
Published: December 2022

Background
Diffuse large B-cell lymphoma (DLBCL) is one of the most aggressive malignant tumors. Chromobox (CBX) family plays the role of oncogenes in various malignancies.
Methods
The transcriptional and protein levels of CBX family were confirmed by GEPIA, Oncomine, CCLE, and HPA database. Screening of co-expressed genes and gene function enrichment analysis were performed by GeneMANIA and DAVID 6.8. The prognostic value, immune cell infiltration and drug sensitivity analysis of CBX family in DLBCL were performed by Genomicscape, TIMER2.0 and GSCALite database. Confirmatory Tests of CBX family protein expression in DLBCL were performed by immunohistochemistry.
Results
The mRNA and protein expression of CBX1/2/3/5/6 were higher in DLBCL tissues than control groups. Enrichment analysis showed that the functions of CBX family were mainly related to chromatin remodeling, methylation-dependent protein binding, and VEGF signaling pathway. The high mRNA expression of CBX2/3/5/6 was identified to be associated with short overall survival (OS) in DLBCL patients. Multivariate COX regression indicated that CBX3 was independent prognostic marker. Immune infiltration analysis revealed that the mRNA expression of CBX family (especially CBX1, CBX5, and CBX6) in DLBCL was significantly correlated with the infiltration of most immune cells (including B cells, CD8 + T cells, CD4 + T cells, neutrophils, monocytes, macrophages, and Treg cells). Meanwhile, there was a strong correlation between the expression levels of CBX1/5/6 and surface markers of immune cells, such as the widely studied PVR-like protein receptor/ligand and PDL-1 immune checkpoint. Notably, our study found that DLBCL cells with CBX1 overexpression was resistant to the common anti-tumor drugs, but CBX2/5 had two polarities. Finally, we confirmed the higher expression of CBX1/2/3/5/6 in DLBCL tissues compared with control groups by immunohistochemistry.
Conclusion
We provided a detailed analysis of the relationship between the CBX family and the prognosis of DLBCL. Distinguished from other studies, We found that high mRNA expression of CBX2/3/5/6 was associated with poor prognosis in DLBCL patients, and Multivariate COX regression indicated that CBX3 was independent prognostic marker. Besides, our study also found an association between the CBX family and antitumour drug resistance, and provided a relationship between CBX family expression and immune cell infiltration.
Ten years of gross alpha/beta measurement interlaboratory comparisons in drinking water
Authors: Yin Liangliang , Xie Yuhan , Qian Yuxin , Ji Yanqin
Published: December 2022

Interlaboratory comparison exercises for determination of activity concentration of gross alpha and gross beta in drinking water organized by the National Institute for Radiological Protection (NIRP), China CDC, have been carried out since 2011 to 2021. The purpose of this work was to assess the accuracy and precision of low level radioactivity concentration for gross alpha and gross beta analysis. The comparisons used the natural water samples and the performance of participating laboratories was evaluated with respect to the reference values using Z-score performance indicator. Over the years, the percentage of laboratories with acceptable results was 80%-92%, the comparison of the acceptable results have increased. The non-acceptance results are mainly due to the uncertainty caused by low detection efficiency and short measuring time.
Ambient pressure storage of high-density methane in carbon nanopores having thermally switchable graphene-locks
Authors: Wang Shuwen , Vallejos-Burgos Fernando , Furuse Ayumi , Marco-Lozar Juan P. , Nagae Miu , Tanaka Hideki , Kanoh Hirofumi , Albero Joaquin Silvestre , Hayashi Takuya , Kaneko Katsumi
Published: December 2022

Physisorption of methane in porous solids has been actively studied to achieve high methane storage capacity, but it requires heavy vessels due to the high pressures involved. In addition, physisorption of methane is quite sensitive to temperature elevation, even near ambient conditions. We developed a method for high-density methane storage at ambient pressure by installing pore-entrance locks controlled by waste heat at 473 K. Under ambient conditions, the entrance locks are stable enough to store a large amount of highly pressurized methane in the carbon nanopores at 19.9 MPa and can be unlocked at 473 K for encapsulation and release of methane. This novel ambient pressure storage method for pressurized methane enables storage of more methane than any other adsorbent at 3.5 MPa and 298 K. The waste-heat open/close pore-entrance locking mechanism supports long-term storage of a renewable energy source and provides a stationary energy supply.
GIS-Based Analytical Hierarchy Process (AHP) for Flood Hazards and Risks Assessment: A Case Study of the Port City of Montego Bay and the surrounding St. James Parish, Jamaica
Authors: McKenzie Ricordo , Gala Tekleab
Published: December 2022

A flood is a hydrological phenomenon of a significant surge in the runoff water that overwhelms and overflows a drain, river, or stream, usually after heavy rainfall, tropical storms, and cyclones. When it occurs, it causes adverse impacts on human life and public health, domestic animals, properties, and croplands. The Caribbean island of Jamaica is located in the geographical area where the current and forecasted hydrological, physical, climatic, and human conditions are set for probable flood hazard occurrences. Therefore, this study aimed at modeling the likelihood of flood hazard occurrences, and risk assessment for Saint James Parish, Jamaica, and its port city of Montego Bay using the Geographic Information Systems (GIS)-based analytical hierarchy process of multicriteria analysis. Accordingly, 36% of the study area is assessed to experience a very high and high probability of flood hazard occurrences. On the other hand, 25% of the study area is subjected to risks of high and very high exposure and vulnerability to the hazard. Wetlands are the most adversely impacted land-use and landcover types (i.e., 100%) followed by Built-up areas (i.e., 74%) and agricultural croplands (24%), indicating the unique vulnerability of the area to the hazards, and damages thereof.
Titania Nanotube Arrays Surface Anodization and Various Human Cell Line Models Adherence Profiles
Authors: Mydin Rabiatul Basria S. M. N. , Effendy Wan Nuramiera Faznie Wan Eddis , Hazan Roshasnorlyza , Romli Nur Afiqah Amalina , Shaharuddin Sadiqah , Khan Ibrahim Hussin , Sreekan Srimala
Published: December 2022

Titania nanotube arrays (TNA) have high biocompatibility, less toxicity, and a large surface area per volume; thus, TNA offer great potential in biomedical applications. Interactions between micro environment and cell on the TNA surface are intensively investigated regarding cell attachment and interaction. Anodization was used to create a highly ordered nano-porous oxide layer with nano-sized pores on the surface of the titanium. This process was carried out at 30 V with a sweep rate of 1 V/sec for a different duration (10 s, 1 min, 2 min, 5 min, 10 min, 20 min, 30 min, 1 h, 2 h, 3 h and 6 h). On an anodized titanium surface, the cell adhesion of several adherence cells was observed using a field emission scanning electron microscope (FESEM). Due to its important role in controlling the morphology of the nanotube structures, extending anodization time causes the length of the nanotubes increases. Hence, the optimised anodization time on the TNA surface at 30 V with a sweep rate of 1 V/s directly impacts cell adhesion after incubating for 48 h. The anodic potential of TNA was successfully obtained at 30 V with a sweep rate of 1 V/sec for 30 min, which could modulate diverse cellular responses of cell adhesion observed by FESEM.
swnK plays an important role in the biosynthesis of swainsonine in Metarhizium anisopliae
Authors: Huang Enxia , Zhang Yu , Sun Lu , Zhu Yiru , Tang Shiyu , Mo Chonghui , Zhao Baoyu , lu hao
Published: December 2022

Swainsonine (SW) is the principal toxic ingredient of locoweeds, and is produced by fungi including Metarhizium anisopliae, Slafractonia leguminicola, and Alternaria oxytropis. A key enzyme in the SW synthesis pathway is a hybrid SWNK/NRPS. The downstream biosynthetic pathways of SW have been clearly analyzed by a knockout and complement of swnK in Metarhizium robertsii, but the upstream steps and the associated regulatory proteins have not been thoroughly investigated. To verify the role of swnK in the SW biosynthesis pathway of M. anisopliae, the concentration of SW and the swnK expression in M. anisopliae fermentation from 1st to 7th d were determined using LC-MS and RT-qPCR, respectively. M. anisopliae had the highest SW content and swnK expression on the 5th day of fermentation; We obtained a mutant strain (MT) by PEG-mediated homologous recombination (HR) to knock out swnK in the wild-type (WT) strain. And we obtained a complemented-type (CT) strain by transforming a modified PUC19 complementation vector containing the geneticin (G418) resistance gene and swnK. SW was not detected in the MT strain and reverted to its original level in the CT strain; We also used a Psilent-1 plasmid with Benomyl (ben)-resistant that interfered with swnK of wild-type strain (WT). The level of SW in the RNAi strain was markedly diminished. RNAi of swnK affected the formation of the cell wall in M. anisopliae. These results indicate that swnK plays a crucial role in the SW biosynthesis of M. anisopliae.
Knowledge, Attitude and Practice Regarding the Factors and Management of Maternal Mortality Amongst the 2nd Year Degree and Diploma Nursing Students at a University Windhoek, Namibia
Authors: Kadhila Joseph Galukeni , Tjomombura Eveline
Published: December 2022

Background: Maternal death is a major health problem in Namibia as it is the case in most countries. The rate at which women are dying due to pregnancy related causes is high and the cause as well as risk factors are not well explored. Despite the introduction of several interventions by the Ministry of Health and Social Services to prevent the maternal deaths, according to the Namibian Demographic Health Survey, the maternal mortality ratio was 249 in 1992, 271 in 2000, 449 in 2006-2007 and 385 in 2013 respectively. It was against this background that the researcher conducted this to determine the knowledge, attitude and practice regarding the factors and management of maternal mortality amongst the 2nd year degree and diploma nursing students at a university, Windhoek, Namibia.
Aims: Assess the knowledge, attitude and practice of 2nd year degree nursing students and diploma nursing students at University regarding the factors and management of maternal mortality.
Objectives: This project is a report of a study to determine the knowledge and practice of 2nd year diploma and degree nursing students regarding factors and their management and to evaluate the attitude and assess the practice of the 2nd year diploma and degree nursing at a University regarding factors and management of maternal mortality
Methodology: In this study Quantitative approach was used so as to collect numerical data from the participants descriptive cross-sectional design was used to obtain data. Quantitative approach is the mathematical method of measuring and describing the observation of materials or characteristics. The target population of this study was the 2nd year diploma and degree student at the University of Namibia, main campus, Windhoek. Data was collected from the 13th of August 2022 to the 12th of October 2022 and was collected online using google forms. Researcher used probability sampling approach as a process of selecting the sample from the population. Descriptive statistics was used to determine the relationships between independent and dependent variables.
Results: A response rate of 54% was achieved. Out of 79 participants, 87.3% (n=69) were female and they were dominating while 12.7% (n=12) were male nursing students. All participants were Namibian. The majority of the participants had grade 12 certificate as the highest qualifications abstained. The study reveals that there was good display of knowledge, practice and attitude on the identification of factors related to maternal mortality.
Conclusion: lack of knowledge, practice and attitude on identification and proper management of factors associated with maternal mortality were revealed in the study. 34.6% of the participants failed to identify puerperal sepsis as an infection that can lead to maternal mortality while 65.4% were able to identify it correctly.
Improving Cotton Simulation Model, GOSSYM, for Soil, Photosynthesis, and Transpiration Processes
Authors: Beegum Sahila , Timlin Dennis , Reddy Kambham Raja , Reddy Vangimalla , Sun Wenguang , Wang Zhuangji , Fleisher David , Ray Chittaranjan
Published: December 2022

GOSSYM, a mechanistic, process-level cotton crop simulation model, has a two-dimensional (2D) gridded soil model called Rhizos that simulates the below-ground processes daily. Water movement is based on gradients of water content and not hydraulic heads. In GOSSYM, photosynthesis is calculated using a daily empirical light response function that requires calibration for response to elevated carbon dioxide (CO2). This report discusses improvements made to the GOSSYM model for soil, photosynthesis, and transpiration processes. GOSSYM's predictions of below-ground processes using Rhizos are improved by replacing it with 2DSOIL, a mechanistic 2D finite element soil process model. The photosynthesis and transpiration model in GOSSYM is replaced with a Farquhar biochemical model and Ball-Berry leaf energy balance model. The newly developed model (modified GOSSYM) is evaluated using field-scale and experimental data from SPAR (soil-plant-atmosphere-research) chambers. Modified GOSSYM better predicted net photosynthesis (root mean square error (RMSE) 25.5 versus 45.2 g CO2 m− 2 day− 1; index of agreement (IA) 0.89 versus 0.76) and transpiration (RMSE 3.3 versus 13.7 liters m− 2 day− 1; IA 0.92 versus 0.14) and improved the yield prediction by 6.0%. Modified GOSSYM improved the simulation of soil, photosynthesis, and transpiration processes, thereby improving the predictive ability of cotton crop growth and development.
Evaluation effect of recombinant lytic polysaccharide monooxygenases added to Aspergillus fumigatus var. niveus cocktail in saccharification of sugarcane bagasse
Authors: Gonçalves Aline Larissa , Cunha Paula Macedo , Lima Awana Silva , Santos Júlio César , Segato Fernando
Published: December 2022

Background: Lignocellulosic biomass is an advantageous alternative to produce biofuels, despite its recalcitrant characteristic. There are microorganisms in nature capable of efficiently degrade biomass, such as the filamentous fungi. Among them, Aspergillus fumigatus var. niveus (AFUMN) has a wide variety of carbohydrate-active enzymes (CAZymes), especially hydrolases, but a low number of oxidative enzymes in its genome. To confirm the enzymatic profile of this fungus, this work analyzed the secretome of AFUMN cultured in sugarcane bagasse as sole carbon source. As expected, the secretome showed a predominance of hydrolytic enzymes compared to the oxidative activity. However, it is known that hydrolytic enzymes act in synergism with oxidative proteins to efficiently degrade cellulose polymer, such as the LPMOs (Lytic Polysaccharide Monooxygenases).
Results: Thus, three LPMOs from the fungus Thermothelomyces thermophilus (TtLPMO9D, TtLPMO9H, and TtLPMO9O) were selected, heterologous expressed in Aspergillus nidulans, purified, and used to complement the AFUMN secretome aiming to evaluate the supplementation effect in the saccharification of sugarcane bagasse process. The saccharification assay was carried out using different concentrations of AFUMN secretome supplemented with recombinant T. thermophilus LPMOs, as well as ascorbic acid as reducing agent for oxidative enzymes.
Conclusions: Through a statistic design created by Design-Expert software, we were capable to analyze a possible cooperative effect between these components. The results obtained indicated that, in general, the addition of TtLPMO9D and ascorbic acid did not favor the conversion process in this study, while TtLPMO9O had a highly significant cooperative effect in bagasse saccharification compared to the control using only AFUMN secretome.
Knowledge, awareness, and worry about human monkeypox and attitude toward its vaccination in non endemic countries: a concise critical review
Authors: LOUNIS Mohamed
Published: December 2022

Background: In May 2022, the world has seen the emergence of human monkeypox, a new zoonotic viral disease in multiple non endemic countries. This health threat has been associated with high worry especially after the COVID-19 catastrophe. Also, the population is exposed to a huge amounts of information making them exposed to false information which could lead to embracing conspiracy theories. Thus evaluating the level of knowledge and the attitude toward vaccination are of great importance to raise them for the categories with low levels of knowledge and acceptance. This scoping review was conducted using PRISMA guidelines with the aim to evaluate the level of knowledge and awareness about monkeypox and the attitude toward its vaccination by selecting studies.
Results: The selection process allowed to include 16 studies in this review. These studies were conducted in 10 countries in Europe and Asia. 9 studies were exclusively related to monkeypox knowledge and awareness, 2 studies were exclusively related to monkeypox vaccines while 5 studies treated the two subjects. These studies were mainly conducted among healthcare workers or among the general population.
Results showed an unsatisfactory level of knowledge and awareness with some categories including physicians and aged individuals as the most informed. Regarding vaccination, Results showed that vaccine hesitancy is still common for both healthcare workers except among Chinese healthcare workers where the rate of vaccine acceptance was estimated at 90.1%.
Conclusions: This review could be helpful in the understanding of the knowledge and awareness and vaccines hesitancy in the first months of the emergence of the HMPX by comparing their evolution in the future studies.
Current status and factors influencing readiness to return to work among young and middle-aged stroke patients in China
Authors: Liu Ziwei , Li Jiaxin , Liu Fangli , Guan Ningxiao , Li Ye , Zhang Yu , Hou Linlin , Jiang Qiuhuan
Published: December 2022

Objectives: Stroke is affecting an increasing number of individuals of working age, and accurate assessment of the readiness to return to work can help to determine the timing of return to work and facilitate an early return to society. The objective of this study was to investigate the current state of readiness to return to work and the factors influencing it among young and middle-aged stroke patients in China.
Materials & Methods: A sample of young and middle-aged stroke patients hospitalized in a tertiary hospital in Henan Province between December 2021 and May 2022 were included in this study. A general information questionnaire and the Readiness to Return to Work Scale, Social Support Rating Scale, Stroke Rehabilitation Self-Efficacy Scale, and Fatigue Severity Scale were administered to the patients.
Results: Of the 203 patients successfully surveyed, 60 (29.6%) were in the pre-intention stage, 35 (17.2%) in the intention stage, 81 (39.9%) in the action-readiness-self-assessment stage, and 27 (13.3%) in the action-readiness-action stage. A logistic regression analysis showed that education level, monthly income, time to start rehabilitation exercise, social support, rehabilitation self-efficacy, and fatigue severity were the main factors affecting the readiness of young and middle-aged stroke patients to return to work.
Conclusion: The readiness of young and middle-aged stroke patients to return to work needs to be increased further. Healthcare professionals should consider the influencing factors of readiness to return to work and design targeted intervention programmes to facilitate successful return to work and normal life.
Study on Interaction and Binding Properties of Terpenes repellent active compounds with Odorant Binding Protein 9B in Tribolium castaneum
Authors: Sang Yu-Li , Qiang Xin-Yue , Dai Lu , Chen Li-Jiang , Liu Yu-Feng , Hao Yan-Jun , Liu Jing-Yu , Qi Jian-Zhong
Published: December 2022

To screen the odorant binding proteins (OBPs) ofTribolium castaneumrelated to repellent activity and explore the repellent mechanism of terpenoids by studying the interaction between ligands andTcOBPs. Behavior tests indicated that (1R)-(+)-α-Pinene andβ-Caryophyllene had repellent activity againstT. castaneum. Through homology modeling and molecular docking, we screenedTcOBP-9B, which is highly expressed in antennae and has strong interactions with (1R)-(+)-α-Pinene,β-Caryophylne, as target protein for recombinantly expressed. Fluorescence spectra and UV absorption spectra showed thatTcOBP-9B had strong bind to (1R)-(+)-α-Pinene andβ-Caryophyllene with concentration-dependent in static quenching. Thermodynamic date revealed that they formed stable complexes through van der Waals force, electrostatic and hydrophobic interactions. By fluorescence competitive binding assay, molecular docking and circular dichroism spectra, the binding sites ofTcOBP-9B was confirmed as theα-helix, where the ligands were competitively bound to 1-NPN. These results suggested thatTcOBP-9B was a significant target protein associated with repellent activity, providing new approach for screening repellents againstT. castaneum.