bluuebunny/crossref_metadata_2025 huggingface dataset:
SRPK1/2 and PP1α exert opposite functions by modulating SRSF1-guided MKNK2 alternative splicing in colon adenocarcinoma
Authors: Liu Hongda , Gong Zheng , Li Kangshuai , Zhang Qun , Xu Zekuan , Xu Yunfei
Published: February 2021

Background

The Mnk2 kinase, encoded by MKNK2 gene, plays critical roles in MAPK signaling and was involved in oncogenesis. Human MKNK2 pre-mRNA can be alternatively spliced into two splicing isoforms, the MKNK2a and MKNK2b, thus yielding Mnk2a and Mnk2b proteins with different domains. The involvement of Mnk2 alternative splicing in colon cancer has been implicated based on RNA-sequencing data from TCGA database. This study aimed at investigating the upstream modulators and clinical relevance of Mnk2 alternative splicing in colon adenocarcinoma (CAC).

Methods

PCR, western blotting and immunohistochemistry (IHC) were performed to assess the expression of Mnk2 and upstream proteins in CAC. The function of Mnk2 and its regulators were demonstrated in different CAC cell lines as well as in xenograft models. Two independent cohorts of CAC patients were used to reveal the clinical significance of MKNK2 alternative splicing.

Results

Comparing with adjacent nontumorous tissue, CAC specimen showed a decreased MKNK2a level and an increased MKNK2b level, which were correlated with KRAS mutation and tumor size. The SRSF1 (serine/arginine-rich splicing factor 1) was further confirmed to be the major splicing factor targeting MKNK2 in CAC cells. Higher expression of SRPK1/2 or decreased activity of PP1α were responsible for enhancing SRSF1 phosphorylation and nucleus translocation, subsequently resulted in a switch of MKNK2 alternative splicing.

Conclusions

Our data showed that phosphorylation and subcellular localization of SRSF1 were balanced by SRPK1/2 and PP1α in CAC cells. High nucleus SRSF1 promoted MKNK2 splicing into MKNK2b instead of MNK2a, consequently enhanced tumor proliferation.
Assessing cross-resistance within the pyrethroids in terms of their interactions with key cytochrome P450 enzymes and resistance in vector populations
Authors: Moyes C. L. , Lees R. S. , Yunta C. , Walker K. J. , Hemmings K. , Oladepo F. , Hancock P. A. , Weetman D. , Paine M. J. I. , Ismail H. M.
Published: February 2021

Background

It is important to understand whether the potential impact of pyrethroid resistance on malaria control can be mitigated by switching between different pyrethroids or whether cross-resistance within this insecticide class precludes this approach.

Methods

Here we assess the relationships among pyrethroids in terms of their binding affinity to, and depletion by, key cytochrome P450 enzymes (hereafter P450s) that are known to confer metabolic pyrethroid resistance in Anopheles gambiae (s.l.) and An. funestus, in order to identify which pyrethroids may diverge from the others in their vulnerability to resistance. We then investigate whether these same pyrethroids also diverge from the others in terms of resistance in vector populations.

Results

We found that the type I and II pyrethroids permethrin and deltamethrin, respectively, are closely related in terms of binding affinity to key P450s, depletion by P450s and resistance within vector populations. Bifenthrin, which lacks the common structural moiety of most pyrethroids, diverged from the other pyrethroids tested in terms of both binding affinity to key P450s and depletion by P450s, but resistance to bifenthrin has rarely been tested in vector populations and was not analysed here. Etofenprox, which also lacks the common structural moiety of most pyrethroids, diverged from the more commonly deployed pyrethroids in terms of binding affinity to key P450s and resistance in vector populations, but did not diverge from these pyrethroids in terms of depletion by the P450s. The analysis of depletion by the P450s indicated that etofenprox may be more vulnerable to metabolic resistance mechanisms in vector populations. In addition, greater resistance to etofenprox was found across Aedes aegypti populations, but greater resistance to this compound was not found in any of the malaria vector species analysed. The results for pyrethroid depletion by anopheline P450s in the laboratory were largely not repeated in the findings for resistance in malaria vector populations.

Conclusion

Importantly, the prevalence of resistance to the pyrethroids α-cypermethrin, cyfluthrin, deltamethrin, λ-cyhalothrin and permethrin was correlated across malaria vector populations, and switching between these compounds as a tool to mitigate against pyrethroid resistance is not advised without strong evidence supporting a true difference in resistance.

Graphical Abstract
Expression of Concern to: Prevalence of needle stick injury and its associated factors among nurses working in public hospitals of Dessie town, Northeast Ethiopia, 2016
Authors: Kebede Awoke , Gerensea Hadgu
Published: February 2021

An amendment to this paper has been published and can be accessed via the original article.
Modulation of atypical brain activation during executive functioning in autism: a pharmacological MRI study of tianeptine
Authors: Wichers Robert H. , Findon James L. , Jelsma Auke , Giampietro Vincent , Stoencheva Vladimira , Robertson Dene M. , Murphy Clodagh M. , Blainey Sarah , McAlonan Grainne , Ecker Christine , Rubia Katya , Murphy Declan G. M. , Daly Eileen M.
Published: February 2021

Background

Autism spectrum disorder (ASD) is associated with deficits in executive functioning (EF), and these have been suggested to contribute to core as well as co-occurring psychiatric symptoms. The biological basis of these deficits is unknown but may include the serotonergic system, which is involved both in regulating EF in neurotypical populations and in the pathophysiology of ASD. We previously demonstrated that reducing serotonin by acute tryptophan depletion (ATD) shifts differences in brain function during performance of EF tasks towards control levels. However, ATD cannot be easily used in the clinic, and we therefore need to adopt alternative approaches to challenge the serotonin system. Hence, we investigated the role of the serotonergic modulator tianeptine on EF networks in ASD.

Method

We conducted a pharmacological magnetic resonance imaging study, using a randomized double-blind crossover design, to compare the effect of an acute dosage of 12.5 mg tianeptine and placebo on brain activation during two EF tasks (of response inhibition and sustained attention) in 38 adult males: 19 with ASD and 19 matched controls.

Results

Under placebo, compared to controls, individuals with ASD had atypical brain activation in response inhibition regions including the inferior frontal cortex, premotor regions and cerebellum. During sustained attention, individuals with ASD had decreased brain activation in the right middle temporal cortex, right cuneus and left precuneus. Most of the case–control differences in brain function observed under placebo conditions were abolished by tianeptine administration. Also, within ASD individuals, brain functional differences were shifted significantly towards control levels during response inhibition in the inferior frontal and premotor cortices.

Limitations

We conducted a pilot study using a single dose of tianeptine, and therefore, we cannot comment on long-term outcome.

Conclusions

Our findings provide the first evidence that tianeptine can shift atypical brain activation during EF in adults with ASD towards control levels. Future studies should investigate whether this shift in the biology of ASD is maintained after prolonged treatment with tianeptine and whether it improves clinical symptoms.
Summary of the proceedings of the International Forum 2020: “Radiologists fighting COVID-19: a united response to a global crisis”
Authors:   European Society of Radiology (ESR)
Published: February 2021

The ESR International Forum at the ECR 2020 discussed the current situation, activities and measures undertaken by radiologists around the world in their fight against the global COVID-19 pandemic. The participating societies were invited to submit written reports detailing the current situation in their countries or regions. The European Society of Radiology (ESR) established the ESR International Forum in order to discuss hot topics in the profession of radiology with non-European radiological partner societies. At the ESR International Forum 2020, different strategies, initiatives, and ideas were presented with regard to facing this unprecedented challenge together.
Hemochromatosis, alcoholism and unhealthy dietary fat: a case report
Authors: Shobi Venkatachalam , Adeseye Awe , Billy Ballard , Medhat Kalliny
Published: February 2021

Background

Hereditary hemochromatosis is an autosomal recessive disorder where the clinical phenotype of skin pigmentation and organ damage occurs only in homozygotes. Simple heterozygotes, that is, just C282Y, typically do not develop iron overload. Here we present a case where a simple heterozygote in combination with alcoholism developed high ferritin and high transferrin saturation levels indicative of iron overload. Though alcoholism alone could explain her presentation, we hypothesize that an inflammatory cocktail of iron and alcohol probably caused our patient to succumb to acute liver failure at a very young age.

Case presentation

A 29-year-old Caucasian woman presented to the hospital with progressively worsening yellowish discoloration of her eyes and skin associated with anorexia, nausea, vomiting, diffuse abdominal discomfort, increasing abdominal girth, dark urine and pale stools for about 2 weeks. Family history was significant for hereditary hemochromatosis. Her father was a simple heterozygote and her grandmother was homozygous for C282Y. Physical examination showed scleral icterus, distended abdomen with hepatomegaly and mild generalized tenderness. Lab test results showed an elevated white blood cell count, ferritin 539 ng/dL, transferrin saturation 58.23%, elevated liver enzymes, elevated international normalized ratio (INR), low albumin, Alcoholic Liver Disease/Nonalcoholic Fatty Liver Disease (ALD/NAFLD) Index (ANI) of 2.6, suggesting a 93.2% probability of alcoholic liver disease, and phosphatidyl ethanol level of 537ng/ml. Genetic testing showed that the patient was heterozygous for human homeostatic iron regulator protein (HFE) C282Y mutation and the normal allele. Computed tomography (CT) of the abdomen revealed hepatomegaly, portal hypertension and generalized anasarca. Magnetic resonance cholangiopancreatography (MRCP) showed negative results for bile duct pathology. Workup for other causes of liver disease was negative. A diagnosis of acute alcoholic hepatitis was made, with Maddrey’s discriminant function of > 32, so prednisolone was started. Her bilirubin and INR continued to increase despite steroids, and the patient unfortunately died.

Conclusion

Our case highlights the importance of considering hemochromatosis in the differential diagnosis of young patients presenting with liver failure, including cases suggestive of alcoholism as the likely etiology. Larger studies are needed to investigate the role of non-iron factors like alcohol and viral hepatitis in the progression of liver disease in simple heterozygotes with hereditary hemochromatosis, given the high prevalence of this mutation in persons of Northern European descent.
Use of platelet concentrate gel in second-intention wound healing: a case report
Authors: Palumbo Vincenzo Davide , Rizzuto Stefano , Damiano Giuseppe , Fazzotta Salvatore , Gottardo Andrea , Mazzola Giuseppina , Lo Monte Attilio Ignazio
Published: February 2021

Background

Wound healing is a complex and dynamic process. Healing of acute and chronic wounds can be impaired by patient factors (that is, comorbidities) and/or wound factors (that is, infection). Regenerative medicine products, such as autologous/homologous platelet-rich plasma gel, may speed up the healing process. Autologous/homologous platelet-rich plasma is an advanced wound therapy used for hard-to-heal acute and chronic wounds. The cytokines and growth factors contained in platelet-rich plasma play a crucial role in the healing process.

Case presentation

A 61-year-old Caucasian male patient, suffering from mental retardation following meningitis, with a transplanted kidney due to prior renal impairment, and under immunosuppressant therapy, was submitted to aneurysmectomy of his proximal left forearm arteriovenous fistula. A few days later, the patient came to our attention with substantial blood loss from the surgical site. The wound presented no signs of healing, and after fistula reparation and considering persistent infection of the surgical site (by methicillin-resistantStaphylococcus aureus), surgeons decided for second-intention healing. To favor healing, 10 mL homologous platelet concentrate gel was sequentially applied. After each application, wound was covered with nonadherent antiseptic dressing. After only seven applications of homologous platelet concentrate gel, wound completely recovered and no amputation was necessary.

Conclusions

Topical application of homologous platelet-rich plasma gel in healing wound shows beneficial results in wound size reduction and induces granulation tissue formation. Platelet-rich plasma could be a safe and cost-effective treatment for managing the cutaneous wound healing process to shorten the recovery period and thereby improve patient quality of life.
Who should be vaccinated first? Comparing vaccine prioritization strategies in Israel and European countries using the Covid-19 Health System Response Monitor
Authors: Cylus Jonathan , Panteli Dimitra , van Ginneken Ewout
Published: February 2021

The rapid rollout of Israel’s vaccination program has led to considerable international interest. In this brief commentary we consider how the criteria for vaccination priority groups differ between Israel and selected European countries. We argue that following the Israeli approach of using broad criteria for prioritization— i.e. having fewer groups and a lower age threshold— could have several beneficial effects, including more manageable logistics and fewer roll out delays, as well as potentially reducing pressure on hospitals. With an increasing supply of vaccines becoming available rapidly in much of Europe, countries could consider following the approach of Israel and adopting broader priority criteria going forward.
Criteria for existence of solutions for a Liouville–Caputo boundary value problem via generalized Gronwall’s inequality
Authors: Mohammadi Hakimeh , Baleanu Dumitru , Etemad Sina , Rezapour Shahram
Published: February 2021

In this research, we first investigate the existence of solutions for a new fractional boundary value problem in the Liouville–Caputo setting with mixed integro-derivative boundary conditions. To do this, Kuratowski’s measure of noncompactness and Sadovskii’s fixed point theorem are our tools to reach this aim. In the sequel, we discuss the continuous dependence of solutions on parameters by means of the generalized Gronwall inequality. Moreover, we consider an inclusion version of the given boundary problem in which we study its existence results by means of the endpoint theory. Finally, we prepare two simulative numerical examples to confirm the validity of the analytical findings.
Multiple solutions for a coercive quasilinear elliptic equation via Morse theory
Authors: Fu Lifang , Sun Mingzheng
Published: February 2021

We study the quasilinear elliptic problem which is resonant at zero. By using Morse theory, we obtain five nontrivial solutions for the equation with coercive nonlinearities.
First order differential systems with a nonlinear boundary condition via the method of solution-regions
Authors: Frigon Marlène , Tella Marcos , F. Tojo F. Adrián
Published: February 2021

In this article we extend the known theory of solution regions to encompass nonlinear boundary conditions. We both provide results for new boundary conditions and recover some known results for the linear case.
Etanercept as a new therapeutic option for cytokine release syndrome following chimeric antigen receptor T cell therapy
Authors: Zhang Lina , Wang Shuai , Xu Ji , Zhang Run , Zhu Han , Wu Yujie , Zhu Liying , Li Jianyong , Chen Lijuan
Published: February 2021

Cytokine release syndrome (CRS) is the most common toxicity induced by chimeric antigen receptor (CAR) T cell therapy. At present, anti-IL-6 agents including tocilizumab and siltuximab have been applied in the treatment of CRS. However, tocilizumab and siltuximab are expensive and some patients fail to respond to anti-IL-6 therapy, which urges the need for new drugs. In clinical practice, we found some patients with multiple myeloma developed markedly increased levels of tumor necrosis factor (TNF)- α during the CRS period after anti-BCMA CAR T cell infusion. Here we present the successful use of TNF-α inhibitor (etanercept) to cure CRS in three patients. The introduction of etanercept did not alter patients' response to CAR T cell therapy and no adverse event was observed directly related to the administration of etanercept. Furthermore, in vitro experiments confirmed that etanercept did not affect the proliferation and effector function of CAR T cells. Our results indicate that etanercept could be considered as a treatment option for CRS in patients with significantly elevated TNF-α levels.
Taxonomic and functional analyses of intact microbial communities thriving in extreme, astrobiology-relevant, anoxic sites
Authors: Bashir Alexandra Kristin , Wink Lisa , Duller Stefanie , Schwendner Petra , Cockell Charles , Rettberg Petra , Mahnert Alexander , Beblo-Vranesevic Kristina , Bohmeier Maria , Rabbow Elke , Gaboyer Frederic , Westall Frances , Walter Nicolas , Cabezas Patricia , Garcia-Descalzo Laura , Gomez Felipe , Malki Mustapha , Amils Ricardo , Ehrenfreund Pascale , Monaghan Euan , Vannier Pauline , Marteinsson Viggo , Erlacher Armin , Tanski George , Strauss Jens , Bashir Mina , Riedo Andreas , Moissl-Eichinger Christine
Published: February 2021

Background

Extreme terrestrial, analogue environments are widely used models to study the limits of life and to infer habitability of extraterrestrial settings. In contrast to Earth’s ecosystems, potential extraterrestrial biotopes are usually characterized by a lack of oxygen.

Methods

In the MASE project (Mars Analogues for Space Exploration), we selected representative anoxic analogue environments (permafrost, salt-mine, acidic lake and river, sulfur springs) for the comprehensive analysis of their microbial communities. We assessed the microbiome profile of intact cells by propidium monoazide-based amplicon and shotgun metagenome sequencing, supplemented with an extensive cultivation effort.

Results

The information retrieved from microbiome analyses on the intact microbial community thriving in the MASE sites, together with the isolation of 31 model microorganisms and successful binning of 15 high-quality genomes allowed us to observe principle pathways, which pinpoint specific microbial functions in the MASE sites compared to moderate environments. The microorganisms were characterized by an impressive machinery to withstand physical and chemical pressures. All levels of our analyses revealed the strong and omnipresent dependency of the microbial communities on complex organic matter. Moreover, we identified an extremotolerant cosmopolitan group of 34 poly-extremophiles thriving in all sites.

Conclusions

Our results reveal the presence of a core microbiome and microbial taxonomic similarities between saline and acidic anoxic environments. Our work further emphasizes the importance of the environmental, terrestrial parameters for the functionality of a microbial community, but also reveals a high proportion of living microorganisms in extreme environments with a high adaptation potential within habitability borders.
Interventional sialendoscopy in parotidomegaly related to eating disorders
Authors: Colella Giuseppe , Lo Giudice Giorgio , De Luca Roberto , Troiano Antonio , Lo Faro Carmelo , Santillo Vincenzo , Tartaro Gianpaolo
Published: February 2021

Background

To evaluate the viability and efficacy of sialendoscopy for the management of parotidomegaly related to eating disorders, 6 patients suffering from eating disorders and recurring symptoms of glandular swelling were followed up at the Multidisciplinary Department of Medical-Surgical and Dental Specialties, Oral and Maxillofacial Surgery Unit, AOU University of Campania “Luigi Vanvitelli”. After the detection of the impaired gland through clinical and radiographical analysis, the diagnostic unit was introduced into the duct and was advanced in, reaching the ductal system. Plaques were washed out, any strictures were dilated both by hydrostatic pressure application and steroid solution injection directly in the fibrotic area.

Results

Both glands resulted affected in 83% of patients. 11 parotid glands were explored and treated. Strictures were found in 2 glands (33%), sialectasis in 3 glands (50%), strictures and sialectasis together in 1 glands (17%). In 3 parotid glands (50%) Stenon’s duct was affected, in two (33%) only secondary ducts, in 1 (17%) both. We reached symptomatic improvement in 5 patients (83%), reporting the spherical volume of the parotid region and pain reduction.

Conclusions

Our results demonstrate that sialendoscopy is a safe and effective therapeutic method to treat EDs salivary symptoms. Treating the underlining psychiatric pathology should be the primary goal in patient care to lower the possible recurrence rate and increase the successful outcome of this technique.
Using multiple case studies of health and justice services to inform the development of a new complex intervention for prison-leavers with common mental health problems (Engager)
Authors: Lennox Charlotte , Stevenson Rachel , Owens Christabel , Byng Richard , Brand Sarah L. , Maguire Mike , Durcan Graham , Stevenson Caroline , Shaw Jenny , Quinn Cath
Published: February 2021

Background

People in the criminal justice system have complex needs but often do not make use of services outside of prison, in many cases due to poorly joined up working between health and criminal justice services. The ‘Engager’ programme aimed to develop a complex collaborative care intervention for people leaving prison with common mental health problems that could support their transition into the community and facilitate joined up working between health, justice and social services. To augment our core intervention theory, we wanted to learn from innovative and forward-thinking services providing interagency support and/or treatment for people experiencing common mental health problems within the criminal justice system. We wanted to identify key elements of interagency practice to understand what was and was not effective in engaging people, maintaining their contact and improving mental health and other aspects of their lives.

Method

We used a multiple case study design with a focused ethnographic approach in four study sites. Data came from three sources (documents, field notes and semi-structured interviews) underwent a framework analysis.

Results

We identified seven main themes, namely: collaboration, client engagement, client motivation, supervision, therapeutic approach, peers and preparations for ending. Engaging and motivating clients was dependent on the relationship built with the professional. This relationship was developed through building trust and rapport, which required time and respectful, open and honest communication. Professionals were often unable to build this relationship effectively if they did not work in effective interagency collaborations, particularly those which included shared practices and were supported by effective supervision.

Conclusions

The multiple case study design contributed insights as to how health and justice services work together. The main themes identified are well known factors in health and justice co-working. However, the novel insights were gleaned examining interdependence and interactions in complex, multifactorial phenomena and practice, in particular the importance of shared practice and supervision models. The approach of selecting a small number of cases representing identified knowledge gaps contributed a valuable addition to the program theory and delivery for an innovative complex intervention.
Being parent of a child with congenital heart disease, what does it mean? A qualitative research
Authors: Nayeri Nahid Dehghan , Roddehghan Zahra , Mahmoodi Farzad , Mahmoodi Parvin
Published: February 2021

Background

Childbirth is one of the invaluable human experiences and is associated with parental happiness. However, when a child is born with congenital heart disease, it creates emotional and mental distress. As a result, it changes the parents’ response to their child birth. Exploring parenthood experiences add to the body of knowledge and reveal new perspectives. In order to make healthcare professionals able to support these children and their families, they should first understand the meaning of this phenomenon. This study aimed to explore the meaning of parenting a child with Congenital Heart Disease in Iran.

Methods

A qualitative study was adopted with a conventional content analysis approach and constant comparative analysis. Participants in this study were 17 parents, including parents of children with congenital heart disease who were selected by purposeful sampling method. Semi-structured interviews were used for data collection and continued to data saturation. Data were analyzed via MAXQDA 10 software.

Results

Four categories and twenty three subcategories emerged as meaning of parenting a child with Congenital Heart Disease. Categories include “Emotional breakdown”, “The catastrophic burden of care”, “Spiritual beliefs of parents” and “The hard road”

Conclusions

Fully understanding the life experience of these families will allow the implementation of targeted health interventions. Hence, by understanding the meaning of parenting a child with Congenital Heart Disease, healthcare professionals can asses parents emotional statues, information and spiritual needs, financial condition, insurance and marital status using CHD standards so that support is individualized, sensitive and time appropriate.
Machine learning-aided risk stratification in Philadelphia chromosome-positive acute lymphoblastic leukemia
Authors: Nishiwaki Satoshi , Sugiura Isamu , Koyama Daisuke , Ozawa Yukiyasu , Osaki Masahide , Ishikawa Yuichi , Kiyoi Hitoshi
Published: February 2021

We used the eXtreme Gradient Boosting algorithm, an optimized gradient boosting machine learning library, and established a model to predict events in Philadelphia chromosome-positive acute lymphoblastic leukemia using a machine learning-aided method. A model was constructed using a training set (80%) and prediction was tested using a test set (20%). According to the feature importance score, BCR-ABL lineage, polymerase chain reaction value, age, and white blood cell count were identified as important features. These features were also confirmed by the permutation feature importance for the prediction using the test set. Both event-free survival and overall survival were clearly stratified according to risk groups categorized using these features: 80 and 100% in low risk (two or less factors), 42 and 47% in intermediate risk (three factors), and 0 and 10% in high risk (four factors) at 4 years. Machine learning-aided analysis was able to identify clinically useful prognostic factors using data from a relatively small number of patients.
A hierarchical machine learning framework for the analysis of large scale animal movement data
Authors: Torney Colin J. , Morales Juan M. , Husmeier Dirk
Published: February 2021

Background

In recent years the field of movement ecology has been revolutionized by our ability to collect high-accuracy, fine scale telemetry data from individual animals and groups. This growth in our data collection capacity has led to the development of statistical techniques that integrate telemetry data with random walk models to infer key parameters of the movement dynamics. While much progress has been made in the use of these models, several challenges remain. Notably robust and scalable methods are required for quantifying parameter uncertainty, coping with intermittent location fixes, and analysing the very large volumes of data being generated.

Methods

In this work we implement a novel approach to movement modelling through the use of multilevel Gaussian processes. The hierarchical structure of the method enables the inference of continuous latent behavioural states underlying movement processes. For efficient inference on large data sets, we approximate the full likelihood using trajectory segmentation and sample from posterior distributions using gradient-based Markov chain Monte Carlo methods.

Results

While formally equivalent to many continuous-time movement models, our Gaussian process approach provides flexible, powerful models that can detect multiscale patterns and trends in movement trajectory data. We illustrate a further advantage to our approach in that inference can be performed using highly efficient, GPU-accelerated machine learning libraries.

Conclusions

Multilevel Gaussian process models offer efficient inference for large-volume movement data sets, along with the fitting of complex flexible models. Applications of this approach include inferring the mean location of a migration route and quantifying significant changes, detecting diurnal activity patterns, or identifying the onset of directed persistent movements.
Rasch testlet model and bifactor analysis: how do they assess the dimensionality of large-scale Iranian EFL reading comprehension tests?
Authors: Geramipour Masoud
Published: February 2021

Rasch testlet and bifactor models are two measurement models that could deal with local item dependency (LID) in assessing the dimensionality of reading comprehension testlets. This study aimed to apply the measurement models to real item response data of the Iranian EFL reading comprehension tests and compare the validity of the bifactor models and corresponding item parameters with unidimensional and multidimensional Rasch models. The data collected from the EFL reading comprehension section of the Iranian national university entrance examinations from 2016 to 2018. Various advanced packages of the R system were employed to fit the Rasch unidimensional, multidimensional, and testlet models and the exploratory and confirmatory bifactor models. Then, item parameters estimated and testlet effects identified; moreover, goodness of fit indices and the item parameter correlations for the different models were calculated. Results showed that the testlet effects were all small but non-negligible for all of the EFL reading testlets. Moreover, bifactor models were superior in terms of goodness of fit, whereas exploratory bifactor model better explained the factor structure of the EFL reading comprehension tests. However, item difficulty parameters in the Rasch models were more consistent than the bifactor models. This study had substantial implications for methods of dealing with LID and dimensionality in assessing reading comprehension with reference to the EFL testing.
Iron loading is a prominent feature of activated microglia in Alzheimer’s disease patients
Authors: Kenkhuis Boyd , Somarakis Antonios , de Haan Lorraine , Dzyubachyk Oleh , IJsselsteijn Marieke E. , de Miranda Noel F. C. C. , Lelieveldt Boudewijn P. F. , Dijkstra Jouke , van Roon-Mom Willeke M. C. , Höllt Thomas , van der Weerd Louise
Published: February 2021

Brain iron accumulation has been found to accelerate disease progression in amyloid-β(Aβ) positive Alzheimer patients, though the mechanism is still unknown. Microglia have been identified as key players in the disease pathogenesis, and are highly reactive cells responding to aberrations such as increased iron levels. Therefore, using histological methods, multispectral immunofluorescence and an automated in-house developed microglia segmentation and analysis pipeline, we studied the occurrence of iron-accumulating microglia and the effect on its activation state in human Alzheimer brains. We identified a subset of microglia with increased expression of the iron storage protein ferritin light chain (FTL), together with increased Iba1 expression, decreased TMEM119 and P2RY12 expression. This activated microglia subset represented iron-accumulating microglia and appeared morphologically dystrophic. Multispectral immunofluorescence allowed for spatial analysis of FTL+Iba1+-microglia, which were found to be the predominant Aβ-plaque infiltrating microglia. Finally, an increase of FTL+Iba1+-microglia was seen in patients with high Aβ load and Tau load. These findings suggest iron to be taken up by microglia and to influence the functional phenotype of these cells, especially in conjunction with Aβ.
Monitoring of a reverse cement job in a high-temperature geothermal environment
Authors: Lipus Martin P. , Reinsch Thomas , Weisenberger Tobias B. , Kragset Steinar , Stefánsson Ari , Bogason Sigurdur G.
Published: February 2021

Cementing operations in wellbores, especially for long casings, are often challenging and prone to deficiencies when not properly planned and executed. While exploring for and exploiting of geothermal resources at temperatures above the critical point of water was attempted in different drilling projects in recent years, the well design, and especially the procedure to run and cement long production casings became a key challenge for drilling engineers. For the first time, a reverse cementing job for a 2.97 km long production casing in a high-temperature geothermal well could be monitored and analyzed using a combination of permanently installed distributed fiber optic and electronic sensors as well as conventional well logging equipment. Data from the permanently installed sensors were used to monitor and evaluate the cementation process as well as the onset of the cement hydration. Based on the data, the understanding of downhole fluid dynamics during cementation could be improved. Our analysis suggests that the cement was diluted during cement placement and partly lost into the formation. These findings can help to better prepare for future drilling ventures under similar downhole conditions.
S-wave experiments for the exploration of a deep geothermal carbonate reservoir in the German Molasse Basin
Authors: Wawerzinek Britta , Buness Hermann , von Hartmann Hartwig , Tanner David C.
Published: February 2021

There are many successful geothermal projects that exploit the Upper Jurassic aquifer at 2–3 km depth in the German Molasse Basin. However, up to now, only P-wave seismic exploration has been carried out. In an experiment in the Greater Munich area, we recorded S-waves that were generated by the conventional P-wave seismic survey, using 3C receivers. From this, we built a 3D volume of P- to S-converted (PS) waves using the asymptotic conversion point approach. By combining the P-volume and the resulting PS-seismic volume, we were able to derive the spatial distribution of the vp/vsratio of both the Molasse overburden and the Upper Jurassic reservoir. We found that the vp/vsratios for the Molasse units range from 2.0 to 2.3 with a median of 2.15, which is much higher than previously assumed. This raises the depth of hypocenters of induced earthquakes in surrounding geothermal wells. The vp/vsratios found in the Upper Jurassic vary laterally between 1.5 and 2.2. Since no boreholes are available for verification, we test our results against an independently derived facies classification of the conventional 3D seismic volume and found it correlates well. Furthermore, we see that low vp/vsratios correlate with high vpand vsvelocities. We interpret the latter as dolomitized rocks, which are connected with enhanced permeability in the reservoir. We conclude that 3C registration of conventional P-wave surveys is worthwhile.
National Health Insurance Fund’s relationship to retail drug outlets: a Tanzania case study
Authors: Embrey Martha , Mbwasi Romuald , Shekalaghe Elizabeth , Liana Jafary , Kimatta Suleiman , Ignace Gasto , Dillip Angel , Hafner Tamara
Published: February 2021

Background

Achieving universal health coverage will require robust private sector engagement; however, as many low- and middle-income countries launch prepayment schemes to achieve universal health coverage, few are covering products from retail drug outlets (pharmacies and drug shops). This case study aims to characterize barriers and facilitators related to incorporating retail drug outlets into national prepayment schemes based on the experience of the Tanzanian National Health Insurance Fund’s (NHIF) certification of pharmacies and accredited drug dispensing outlets.

Methods

We reviewed government documents and interviewed 26 key informants including retail outlet owners and dispensers and central and district government authorities representing eight districts overall. Topics included awareness of NHIF in the community, access to medicines, claims processing, reimbursement prices, and how the NHIF/retail outlet linkage could be improved.

Results

Important enablers for NHIF/retail outlet engagement include widespread awareness of NHIF in the community, NHIF’s straightforward certification process, and their reimbursement speed. All of the retail respondents felt that NHIF helps their business and their clients to some degree. As for barriers, retailers thought that NHIF needed to provide more information to them and to its members, particularly regarding coverage changes. Some retailers and government officials thought that the product reimbursement prices were below market and not adjusted often enough, and pharmacy respondents were unhappy about claim rejections for what they felt were insignificant issues. All interviewees agreed that one of the biggest problems is poor prescribing practices in public health facilities. They reiterated that prescribers need more supervision to improve their practices, particularly to ensure adherence to standard treatment guidelines, which NHIF requires for approving a claim. In addition, if a prescription has any problem, including a wrong date or no signature, the client must return to the health facility to get it corrected or pay out-of-pocket, which is burdensome.

Conclusions

Little published information is available on the relationship between health insurance plans and retail providers in low- and middle-income countries. This case study provides insights that countries can use when designing ways to include retail outlets in their health insurance schemes.
Application of MXenes in environmental remediation technologies
Authors: Tunesi Mawada Mohammed , Soomro Razium Ali , Han Xi , Zhu Qizhen , Wei Yi , Xu Bin
Published: February 2021

MXenes have recently been recognized as potential materials based on their unique physical and chemical characteristics. The widely growing family of MXenes is rapidly expanding their application domains since their first usage as energy materials was reported in 2011. The inherent chemical nature, high hydrophilicity, and robust electrochemistry regard MXenes as a promising avenue for environment-remediation technologies such as adsorption, membrane separation, photocatalysis and the electrocatalytic sensor designed for pollutant detection. As the performance of MXenes in these technologies is on a continuous path to improvement, this review intends to cumulatively discuss the diversity and chemical abilities of MXenes and their hybrid composites in the fields mentioned above with a focus on MXenes improving surface-characteristics. The review is expected to promote the diversity of MXenes and their hybrid configuration for advanced technologies widely applied for environmental remediation.
Xylanolytic Bacillus species for xylooligosaccharides production: a critical review
Authors: Rashid Rozina , Sohail Muhammad
Published: February 2021

The capacity of different Bacillus species to produce large amounts of extracellular enzymes and ability to ferment various substrates at a wide range of pH and temperature has placed them among the most promising hosts for the industrial production of many improved and novel products. The global interest in prebiotics, for example, xylooligosaccharides (XOs) is ever increasing, rousing the quest for various forms with expanded productivity. This article provides an overview of xylanase producing bacilli, with more emphasis on their capacity to be used in the production of the XOs, followed by the purification strategies, characteristics and application of XOs from bacilli. The large-scale production of XOs is carried out from a number of xylan-rich lignocellulosic materials by chemical or enzymatic hydrolysis followed by purification through chromatography, vacuum evaporation, solvent extraction or membrane separation methods. Utilization of XOs in the production of functional products as food ingredients brings well-being to individuals by improving defense system and eliminating pathogens. In addition to the effects related to health, a variety of other biological impacts have also been discussed.
Prospective Observational Study of Weight-based Assessment of Sodium Supplements on Ultramarathon Performance (WASSUP)
Authors: Lipman Grant S. , Hew-Butler Tamara , Phillips Caleb , Krabak Brian , Burns Patrick
Published: February 2021

Background

Sodium supplements are ubiquitous in endurance running, but their impact on performance has been subjected to much debate. The objective of the study was to assess the effect of sodium supplementation as a weight-based predictor of race performance in ultramarathon runners.

Methods

Prospective observational study during an 80 km (50 mi) stage of a 6-stage 250 km (155 mi) ultramarathon in Chile, Patagonia, Namibia, and Mongolia. Finish line hydration status as measured by weight change, point-of-care serum sodium, and questionnaire provided sodium ingestion categories at 33rd percentile and 66th percentile both for weight-adjusted rate and total sodium consumption, then analyzed for significant relationships to race performance, dysnatremia, and hydration.

Results

Two hundred sixty-six participants were enrolled, with 217 (82%) with complete sodium supplement rate data, 174 (80%) with finish line sodium, and 161 (74%) with both pre-race weights and total sodium ingestion allowing weight-based analysis. Sodium intake ranged from 131–533 mg/h/kg (2–7.2 gm), with no statistically significant impact on pace, race time, or quintile rank. These outcomes did not change when sodium intake was analyzed as a continuous variable or by sub-group analysis of the 109 (68%) normonatremic runners. When controlled for weight-adjusted sodium intake, performance was poorly correlated with hydration (r= − 0.152, 95% CI − 0.348–0.057). Dehydrated runners outperformed those overhydrated, with 11% of top 25th percentile finishers dehydrated (versus 2.8% overhydrated), with 3.6 min/km faster pace and time 4.6 h faster finishing time.

Conclusions

No association was found between sodium supplement intake and ultramarathon performance. Dehydrated runners were found to have the best performance. This reinforces the message to avoid overhydration.
Prophylactic perioperative cefuroxime levels in plasma and adipose tissue at the time of caesarean section (C-LACE): a protocol for a pilot experimental, prospective study with non-probability sampling to determine interpatient variability
Authors: Alrammaal Hanadi H. , Batchelor Hannah K. , Chong Hsu P. , Hodgetts Morton Victoria , Morris R. Katie
Published: February 2021

Background

The aim of the C-LACE study is to measure cefuroxime concentration in plasma and adipose tissue of non-obese and obese pregnant women undergoing caesarean section.

Methods

This study plans to compare maternal cefuroxime concentrations (plasma and adipose tissue), at the time of skin incision and time of skin closure during a caesarean section from non-obese (body mass index BMI < 30 kg/m2) and obese (BMI ≥ 30 kg/m2) pregnant women. The incidence of post-surgical site infection will also be measured. At least 15 participants are required for each arm (non-obese vs obese) with a total of 30 participants. The study participants will be followed up between 30 and 40 days post-caesarean section to record details of any post-caesarean surgical infection to explore correlations between BMI, measured cefuroxime concentrations and post-caesarean infection rates.

Discussion

This pilot study will allow the development of a model testing the inter-patient variability in plasma and adipose tissue concentrations of cefuroxime. The results will facilitate the development of a larger study to determine whether differences in cefuroxime plasma and tissue concentration in obese and non-obese women can support the development of a physiologically based pharmacokinetic model. This model can then be used to propose dosing adjustments that can be used in a further trial to optimise cefuroxime dosing for women undergoing caesarean section.

Trial registration

ISRCTN Registry, ISRCTN17527512. Registered on 26 October 2020
Proteolytic enzyme arbitrated antagonization of helminthiasis by Cinnamomum cappara leaf extract in Pheretima posthuma
Authors: Vadakkan Kayeen , Cheruvathur Meena K , Chulliparambil Anu S , Francis Famy , Abimannue Anu P
Published: February 2021

Background

There have been several studies carried out to irradiate Helminthiasis however very little research have been carried out where in the enzymatic activity of plants are exploited to antagonize infections. Here we are analyzing the anthelmintic activity of Cinnamomum cappara leaf extract against Pheretima posthuma complimented by proteolytic action.

Results

The fresh leaves of Cinnamomum cappara was collected from local areas of Thrissur during December 2019. Plants were identified and authenticated by morphological and molecular characterization. The enzymatic action was analyzed by plotting Lineweaver–Burk plot which suggested that the extract possess the Km 185.77 μM for casein as substrate and obeyed Michaelis–Menten kinetics with typical hyperbolic relation with enzyme and increasing concentration of substrate. The effect of extract upon study subject was in directly proportional with concentration of antagonist where higher activities were obtained in high concentrations. The anatomical and histological studies suggested that the activity of extract was due to the degradation of muscular bundle of subject that resulted in the leakage of ceolomic fluid.

Conclusions

Cinnamomum cappara leaf extract possessed high degree of protease intervened anthelmintic activity against Pheretima posthuma. As the study subject show immense morphological and physiological resemblance with all other helminthic parasites, this results shall be adopted to further clinical and pharmacological applications.