bluuebunny/crossref_metadata_2025 huggingface dataset:
“It must start with me, so it started with me”: A qualitative study of Project YES! youth peer mentor implementing experiences supporting adolescents and young adults living with HIV in Ndola, Zambia
Authors: Burke Virginia M. , Frimpong Christiana , Miti Sam , Mwansa Jonathan K. , Abrams Elizabeth A. , Merrill Katherine G. , Denison Julie A.
Published: February 2022

Background

Little is known about youth-led approaches to addressing HIV-related outcomes among adolescents and young adults (AYA) living with HIV. In response, Project YES! hired and trained youth living with HIV as peer mentors (YPMs) in four HIV clinics in Ndola, Zambia to hold meetings with 276 15-24-year-olds living with HIV. Within this randomized controlled trial, a qualitative sub-study was conducted to explore YPMs’ implementing experiences.

Methods

In-depth interviews were conducted with the eight YPMs (50% female) ages 21–26 years. YPMs were asked about their experiences working with clients, their feedback on program components, and what the experience meant to them personally and professionally. Interviews were audio-recorded, transcribed verbatim, and thematic analysis was performed.

Results

YPMs connected with AYA clients by discussing shared struggles, modeling positive health behaviors, and establishing judgement-free environments. YPMs experienced powerful personal transformations in HIV-related health behaviors, conceptions of self, and plans for the future. Many expressed now seeing themselves as community leaders–“ambassadors”, “game changers”–and “not just alone in this world.” They described newfound commitments to reaching personal and professional goals. YPMs were adamant that Project YES! should expand so other HIV-positive AYA might benefit.

Conclusion

Well-trained and compensated YPMs who are integrated into HIV clinics can support AYA in unique and important ways due to their shared experiences. The transformational experience of becoming YPMs empowers youth to see themselves as role models and leaders. Future programs should engage youth living with HIV as partners in efforts to end the HIV epidemic.
Burkholderia pseudomallei pathogenesis in human skin fibroblasts: A Bsa type III secretion system is involved in the invasion, multinucleated giant cell formation, and cellular damage
Authors: Kaewpan Anek , Duangurai Taksaon , Rungruengkitkun Amporn , Muangkaew Watcharamat , Kanjanapruthipong Tapanee , Jitprasutwit Niramol , Ampawong Sumate , Sukphopetch Passanesh , Chantratita Narisara , Pumirat Pornpan
Published: February 2022

Burkholderia pseudomallei—a causative agent of melioidosis that is endemic in Southeast Asia and Northern Australia—is a Gram-negative bacterium transmitted to humans via inhalation, inoculation through skin abrasions, and ingestion. Melioidosis causes a range of clinical presentations including skin infection, pneumonia, and septicemia. Despite skin infection being one of the clinical symptoms of melioidosis, the pathogenesis of B. pseudomallei in skin fibroblasts has not yet been elucidated. In this study, we investigated B. pseudomallei pathogenesis in the HFF-1 human skin fibroblasts. On the basis of co-culture assays between different B. pseudomallei clinical strains and the HFF-1 human skin fibroblasts, we found that all B. pseudomallei strains have the ability to mediate invasion, intracellular replication, and multinucleated giant cell (MNGC) formation. Furthermore, all strains showed a significant increase in cytotoxicity in human fibroblasts, which coincides with the augmented expression of matrix metalloproteinase-2. Using B. pseudomallei mutants, we showed that the B. pseudomallei Bsa type III secretion system (T3SS) contributes to skin fibroblast pathogenesis, but O-polysaccharide, capsular polysaccharide, and short-chain dehydrogenase metabolism do not play a role in this process. Taken together, our findings reveal a probable connection for the Bsa T3SS in B. pseudomallei infection of skin fibroblasts, and this may be linked to the pathogenesis of cutaneous melioidosis.
Dairy farmer practices and attitudes relating to pasture-based and indoor production systems in Scotland
Authors: Shortall Orla K. , Lorenzo-Arribas Altea
Published: February 2022

This study assesses the practices and views of Scottish dairy farmers relating to pasture-based and indoor systems. There are the debates about the environmental, economic and animal welfare implications of these systems. Indoor dairy farming is a contentious practice among the public. While this controversy is sometimes represented as a lack of public understanding, there is a need for more research on farmers’ views to facilitate discussion in the industry. A survey was posted to 909 dairy farmers in Scotland with questions about their grazing practices and attitudes to grazing and indoor systems. 254 surveys were completed, online and in paper form. There was a 26% response rate to the paper version of the survey. The results showed that 19% of respondents housed some or all the cows all year-round. 68% agreed or strongly agreed that cows should graze for part of the year and 51% agreed or strongly agreed that welfare was better if cows grazed for part of the year. These views coexisted with the view that management was more important than the type of system for determining profitability or welfare outcomes (83% and 82% strongly agree or agree respectively). Respondents whose system involved grazing and respondents who had spent longer in farming were moderately more likely to agree that cows should have access to pasture, and slightly less likely to agree that management was more important than system for determining welfare outcomes. The results indicate that the picture is more complicated than the public rejecting indoor dairy farming and those in the industry accepting it. The results showed that a majority preference for cows to graze co-existed with the view that management was more important than system. In terms of industry and policy recommendations, the research suggests that measures should be taken to safeguard farmers’ ability to graze through for instance research and advisory support on grazing; ensuring different systems are not penalised in the development of dairy sector environmental measures and recommendations; and potentially supply chains that financially rewards farmers for grazing.
Help seeking behavior by women experiencing intimate partner violence in india: A machine learning approach to identifying risk factors
Authors: Dehingia Nabamallika , Dey Arnab K. , McDougal Lotus , McAuley Julian , Singh Abhishek , Raj Anita
Published: February 2022

Background

Despite the low prevalence of help-seeking behavior among victims of intimate partner violence (IPV) in India, quantitative evidence on risk factors, is limited. We use a previously validated exploratory approach, to examine correlates of help-seeking from anyone (e.g. family, friends, police, doctor etc.), as well as help-seeking from any formal sources.

Methods

We used data from a nationally-representative health survey conducted in 2015–16 in India, and included all variables in the dataset (~6000 variables) as independent variables. Two machine learning (ML) models were used- L-1, and L-2 regularized logistic regression models. The results from these models were qualitatively coded by researchers to identify broad themes associated with help-seeking behavior. This process of implementing ML models followed by qualitative coding was repeated until pre-specified criteria were met.

Results

Identified themes associated with help-seeking behavior included experience of injury from violence, husband’s controlling behavior, husband’s consumption of alcohol, and being currently separated from husband. Themes related to women’s access to social and economic resources, such as women’s employment, and receipt of maternal and reproductive health services were also noted to be related factors. We observed similarity in correlates for seeking help from anyone, vs from formal sources, with a greater focus on women being separated for help-seeking from formal sources.

Conclusion

Findings highlight the need for community programs to reach out to women trapped in abusive relationships, as well as the importance of women’s social and economic connectedness; future work should consider holistic interventions that integrate IPV screening and support services with women’s health related services.
High level SARS-CoV-2 nucleocapsid refolding using mild condition for inclusion bodies solubilization: Application of high pressure at pH 9.0
Authors: Chura-Chambi Rosa Maria , Prieto-da-Silva Alvaro Rossan de Brandão , Di Lela Matheus Martins , Oliveira João Ezequiel , Abreu Patricia Estima Antonia , Meireles Luciana Regina , de Andrade Junior Heitor Franco , Morganti Ligia
Published: February 2022

SARS-CoV-2 Nucleocapsid (N) is the most abundant viral protein expressed in host samples and is an important antigen for diagnosis. N is a 45 kDa protein that does not present disulfide bonds. Intending to avoid non-specific binding of SARS-CoV-2 N to antibodies from patients who previously had different coronaviruses, a 35 kDa fragment of N was expressed without a conserved motif in E. coli as inclusion bodies (N122-419-IB). Culture media and IB washing conditions were chosen to obtain N122-419-IB with high yield (370 mg/L bacterial culture) and protein purity (90%). High pressure solubilizes protein aggregates by weakening hydrophobic and ionic interactions and alkaline pH promotes solubilization by electrostatic repulsion. The association of pH 9.0 and 2.4 kbar promoted efficient solubilization of N122-419-IB without loss of native-like tertiary structure that N presents in IB. N122-419 was refolded with a yield of 85% (326 mg/L culture) and 95% purity. The refolding process takes only 2 hours and the protein is ready for use after pH adjustment, avoiding the necessity of dialysis or purification. Antibody binding of COVID-19-positive patients sera to N122-419 was confirmed by Western blotting. ELISA using N122-419 is effective in distinguishing between sera presenting antibodies against SARS-CoV-2 from those who do not. To the best of our knowledge, the proposed condition for IB solubilization is one of the mildest described. It is possible that the refolding process can be extended to a wide range of proteins with high yields and purity, even those that are sensible to very alkaline pH.
Heterogeneity estimates in a biased world
Authors: Hönekopp Johannes , Linden Audrey Helen
Published: February 2022

Meta-analyses typically quantify heterogeneity of results, thus providing information about the consistency of the investigated effect across studies. Numerous heterogeneity estimators have been devised. Past evaluations of their performance typically presumed lack of bias in the set of studies being meta-analysed, which is often unrealistic. The present study used computer simulations to evaluate five heterogeneity estimators under a range of research conditions broadly representative of meta-analyses in psychology, with the aim to assess the impact of biases in sets of primary studies on estimates of both mean effect size and heterogeneity in meta-analyses of continuous outcome measures. To this end, six orthogonal design factors were manipulated: Strength of publication bias; 1-tailed vs. 2-tailed publication bias; prevalence ofp-hacking; true heterogeneity of the effect studied; true average size of the studied effect; and number of studies per meta-analysis. Our results showed that biases in sets of primary studies caused much greater problems for the estimation of effect size than for the estimation of heterogeneity. For the latter, estimation bias remained small or moderate under most circumstances. Effect size estimations remained virtually unaffected by the choice of heterogeneity estimator. For heterogeneity estimates, however, relevant differences emerged. For unbiased primary studies, the REML estimator and (to a lesser extent) the Paule-Mandel performed well in terms of bias and variance. In biased sets of primary studies however, the Paule-Mandel estimator performed poorly, whereas the DerSimonian-Laird estimator and (to a slightly lesser extent) the REML estimator performed well. The complexity of results notwithstanding, we suggest that the REML estimator remains a good choice for meta-analyses of continuous outcome measures across varied circumstances.
Mineral biofortification of vegetables through soil-applied poultry mortality compost
Authors: Mubarak Muhammad Umair , Kiran Aysha , Shahzad Ahmad Naeem , Qayyum Muhammad Farooq , Ishfaq Muhammad , Mahmood Khalid , Wakeel Abdul
Published: February 2022

Intensive agricultural practices lower soil fertility, particularly micronutrients which are rarely applied to soils as chemical fertilizers. Micronutrient deficiency in soils results in inferior product quality and micronutrient malnutrition in humans. Application of compost to soil may improve crop yields and quality by enhancing macro- and micronutrients availability, enhancing soil microbial population, and improving soil physicochemical properties. Poultry mortality compost (PMC) was prepared by decomposing dead poultry birds with poultry litter in an aerated bin through indigenous microbial populations. The prepared PMC was used as an amendment in three field experiments during 2017–18 and 2018–19 to investigate the effect on yield and nutritional quality of potato, carrot, and radish. In these field trials, two compost levels, i.e., 1250 kg ha-1(PMC1) and 1850 kg ha-1(PMC2) were compared with the control (no compost application). The results revealed a 10–25% increase in root or tuber yield at PMC2 compared to that in the control. A substantial increase in Zn, Fe, and Mn concentrations in vegetable root/tubers was also observed. Organic matter content and microbial biomass were improved in the soil with PMC application leading to better soil health and better nutrient availability. These studies led us to conclude that the application of PMC not only enhances the vegetable yield but also biofortifies vegetables with micronutrients such as Zn, Fe, and Mn extending agricultural sustainability and eliminating micronutrient malnutrition in humans.
Perspectives on preparing for long-acting injectable treatment for HIV among consumer, clinical and nonclinical stakeholders: A qualitative study exploring the anticipated challenges and opportunities for implementation in Los Angeles County
Authors: Jolayemi Oluwadamilola , Bogart Laura M. , Storholm Erik D. , Goodman-Meza David , Rosenberg-Carlson Elena , Cohen Rebecca , Kao Uyen , Shoptaw Steve , Landovitz Raphael J.
Published: February 2022

Long-acting injectable (LAI) antiretroviral therapy (ART) is a novel HIV treatment option for people with HIV. The first LAI ART regimen for HIV treatment received regulatory approval in the United States in January 2021. In February 2020, we collected qualitative data from 18 consumers and 23 clinical and non-clinical stakeholders to catalog anticipated individual-consumer, healthcare system, and structural levels barriers and facilitators to LAI ART implementation in Los Angeles County, California. Thematic analysis was guided by the CFIR implementation science model. CFIR constructs of intervention characteristics, individual characteristics, outer and inner setting, intervention characteristics, and implementation process emerged in analysis. Under intervention characteristics, anticipated facilitators included the relative advantage of LAI ART over pills for adherence and reduced treatment management burden and related anxiety; anticipated barriers included non-adherence to injection appointments, concerns of developing HIV resistance, discomfort with injection and cost. Anticipated facilitators based on individual characteristics included overall acceptability based on knowledge and positive beliefs about LAI ART. Participant noted several characteristics of the outer setting that could negatively impact implementation, such as medical mistrust, external policies, and LAI ART eligibility (i.e., to be virally suppressed prior to initiation). Participants were optimistic about the potential to decrease stigma but expressed that provider willingness for adoption could be hindered by challenges in organizational inner setting related to payment authorizations, increased staffing needs, medication procurement and storage, and provider and healthcare system readiness. Results from this pre-implementation study may inform rollout and scale-up of LAI ART in Los Angeles County.
The Gyaros island marine reserve: A biodiversity hotspot in the eastern Mediterranean Sea
Authors: Damalas Dimitrios , Stamouli Caterina , Fotiadis Nikolaos , Kikeri Maria , Kousteni Vasiliki , Mantopoulou-Palouka Danai
Published: February 2022

Since July 2019, Gyaros island in the central Aegean Sea, enjoys the status of a partial Marine Protected Area (MPA), allowing for exploitation by small-scale fishers following specific spatio-temporal restrictions. The need for assessing the effectiveness of the MPA in the future, led MAVA Foundation to fund a knowledge survey project aiming to serve as a baseline for future reference. A series of experimental fishing surveys took place with static nets, the outcomes of which are presented herein. From June 2018 to September 2020, a series of 8 fishing excursions with a total of 40 experimental fishing sets with bottom static nets were realized in 5 set locations around Gyaros island, inside the MPA protection zone. A total of 75 species were identified; the most abundant species, in terms of biomass, being: parrotfish-Sparisoma cretense, red scorpionfish-Scorpaena scrofa, common spiny lobster-Palinurus elephas, red porgy-Pagrus pagrus, little tunny–-Euthynnus alletteratus, Mediterranean moray-Muraena helena, lesser spotted dogfish -Scyliorhinus canicula, forkbeard-Phycis phycis, surmullet-Mullus surmuletus, common cuttlefish-Sepia officinalis and common Pandora-Pagellus erythrinus. A comparison with similar data in adjacent areas outside the MPA allowed for assessing the effectiveness of the MPA based on four indicators: species diversity index, species relative biomass index, key predator species abundance, and alien fish abundance. Based solely on the experimental fishing trials, the MPA seems to be functioning, since both species diversity and abundance were higher within the protected area. However, its performance may still not be considered as optimal, as this is indicated by the large proportion of undersized key predators (e.g. groupers), although more abundant and larger than the ones residing outside the MPA.
Cost-effectiveness of the TherMax blood warmer during continuous renal replacement therapy
Authors: Blackowicz Michael J. , Bell Max , Echeverri Jorge , Harenski Kai , Broman Marcus E.
Published: February 2022

Hypothermia is a common adverse event during continuous renal replacement therapy (CRRT), affecting multiple organ systems and increasing risk of poor health outcomes among patients with acute kidney injury (AKI) undergoing CRRT. TheraMax blood warmers are the next generation of extracorporeal blood warmers which reduce risk of hypothermia during CRRT. The purpose of this study is to elucidate the potential health economic impacts of avoiding CRRT-induced hypothermia by using the novel TherMax blood warming device. This study compares health care costs associated with use of the new TherMax blood warmer unit integrated with the PrisMax system compared to CRRT with a standalone blood warming device to avoid hypothermia in continuous renal replacement therapy (CRRT). An economic model was developed in which relevant health states for each intervention were normothermia, hypothermia, discharge, and death. Clinical inputs and costs were obtained from a combination of retrospective chart review and publicly available summary estimates. The proportion of AKI patients treated with CRRT who became hypothermic (<36°C) during CRRT treatment was 34.5% in the TherMax group compared to 71.9% in the ‘standalone warmer’ group. Given the 78.7-year average life expectancy in the US and the assumed average patient age at discharge/death of 65.4 years, the total life-years gained by avoiding mortality related to hypothermia was 9.0 in the TherMax group compared to 8.0 in the ‘standalone warmer’ group. Cost per life-year gained was $8,615 in the TherMax group versus $10,115 in the ‘standalone warmer’ group for a difference of -$1,501 favoring TherMax. The incremental cost-effectiveness ratio was negative, indicating superior cost-effectiveness for TherMax versus ‘standalone warmer’. The TherMax blood warming device used with the PrisMax system is associated with lower risk of hypothermia, which our model indicates leads to lower costs, lower risk of mortality due to hypothermia, and superior cost-effectiveness.
Projections of the economic burden of care for individuals with dementia in mainland China from 2010 to 2050
Authors: Huang Yixiang , Li Xiande , Liu Zifeng , Huo Jinhai , Guo Jianwei , Chen Yingying , Chen Yanmei , Chen Ruoling
Published: February 2022

Background

China has stepped into an era of aging society, where the impending considerable economic burden attributed to high prevalence of dementia in the elderly appears to be one of the most important health and social issues to deal with for the country. However, population-based quantification and projections for the economic burden of dementia in China are lacking for further health action and policy making.

Objective

To estimate and predict the costs of managing dementia in the elderly population aged 60 and above from 2010 to 2050 in China.

Methods

Data were collected from a six-province study (n = 7072) and other multiple sources for calculation of the economic burden of dementia. With the convincing data from published studies, we quantified and projected the costs attributed to dementia in China from 2010 to 2050.

Results

The national cost of dementia in 2010 was estimated to be US$22.8 billion by the opportunity cost method and US$26.4 billion by the proxy method. In 2050, the costs would increase to US$372.3 billion by the opportunity cost method and US$430.6 billion by the proxy method, consuming 0.53% and 0.61% of China’s total GDP, respectively. A series of sensitivity analyses showed that the changes in the proportions of informal caregiving led to the most robust changes in the total burden of care for dementia in China.

Conclusion

Dementia represents an enormous burden on China’s population health and economy. Due to the changes in policies and population structure, policymakers should give priority to dementia care.
Combination of Xpert® MTB/RIF and DetermineTM TB-LAM Ag improves the diagnosis of extrapulmonary tuberculosis at Jimma University Medical Center, Oromia, Ethiopia
Authors: Simieneh Asnake , Tadesse Mulualem , Kebede Wakjira , Gashaw Mulatu , Abebe Gemeda
Published: February 2022

Background

Ethiopia is one of the high burden countries for extrapulmonary tuberculosis (EPTB); however, the prompt diagnosis of EPTB remains challenging. This study is aimed to evaluate the diagnostic performance of Xpert MTB/RIF and DetermineTM TB-LAM Ag (TB-LAM) for the prompt diagnosis of EPTB in Ethiopia.

Methods

A total of 147 presumptive EPTB patients, including 23 HIV- positive participants were enrolled. Extra-pulmonary samples were collected from all presumptive EPTB cases and tested for Mycobacterium tuberculosis complex (MTBC) using fluorescent microscopy, Xpert MTB/RIF, and culture. Additionally, urine samples were also collected from 126 participants and were tested by DetermineTM TB-LAM Ag (Alere Inc, Waltham, USA). The Sensitivity and specificity of Xpert and TB- LAM tests were calculated by comparing with a composite reference standard (CRS), which comprises smear microscopy, culture and response to empirical anti-TB treatment.

Results

Of 147 patients, 23 (15.6%) were confirmed EPTB cases (culture-positive), 14 (9.5%) were probable EPTB (clinically, radiologically or cytologically positive and received anti-TB treatment with good response), and 110 (74.8%) were classified as “non- TB” cases. Compared to the composite reference standard (CRS), the overall sensitivity and specificity of Xpert MTB/RIF were 43.2% and 100%, respectively with the highest sensitivity for Lymph node aspirate (85.7%) and lower sensitivity for pleural fluid (14.3%) and 100% specificity for all specimen types. The sensitivity and specificity of TB-LAM were 33.3% and 94.4% respectively with the highest sensitivity for HIV co-infected participants (83.3%). The sensitivity of the combination of Xpert MTB/RIF and TB-LAM tests regardless of HIV status was 61.1% whereas the sensitivity was improved to 83.3% for HIV-positive cases.

Conclusion

TB-LAM alone has low sensitivity for EPTB diagnosis; however, the combination of TB-LAM and Xpert MTB/RIF improves the diagnosis of EPTB particularly for countries with high EPTB and HIV cases.
Physical exercise is associated with a reduction in plasma levels of fractalkine, TGF-β1, eotaxin-1 and IL-6 in younger adults with mobility disability
Authors: Kumar Parvin , Stiernborg Miranda , Fogdell-Hahn Anna , Månsson Kristoffer , Furmark Tomas , Berglind Daniel , Melas Philippe A. , Forsell Yvonne , Lavebratt Catharina
Published: February 2022

Mobility disability (MD) refers to substantial limitations in life activities that arise because of movement impairments. Although MD is most prevalent in older individuals, it can also affect younger adults. Increasing evidence suggests that inflammation can drive the development of MD and may need to be targeted for MD prevention. Physical exercise has anti-inflammatory properties and has been associated with MD prevention. However, no studies to date have examined whether exercise interventions affect the peripheral inflammatory status in younger adults with MD. To this end, we used blood samples from young and middle-aged adults with MD (N = 38; median age = 34 years) who participated in a 12-week intervention that included aerobic and resistance exercise training. A pre-post assessment of inflammatory biomarkers was conducted in plasma from two timepoints, i.e., before the exercise trial and at follow-up (3–7 days after the last exercise session). We successfully measured 15 inflammatory biomarkers and found that exercise was associated with a significant reduction in levels of soluble fractalkine, transforming growth factor beta 1 (TGF-β1), eotaxin-1 and interleukin (IL) 6 (corrected α = 0.004). We also found significant male-specific effects of exercise on (i) increasing IL-16 and (ii) decreasing vascular endothelial growth factor-A (VEGF-A). In line with our results, previous studies have also found that exercise can reduce levels of TGF-β1, eotaxin-1 and IL-6. However, our finding that exercise reduces plasma levels of fractalkine in younger adults with MD, as well as the sex-dependent findings, have not been previously reported and warrant replication in larger cohorts. Given the suggested role of inflammation in promoting MD development, our study provides additional support for the use of physical exercise as a treatment modality for MD.
Microplastics prevalence in water, sediment and two economically important species of fish in an urban riverine system in Ghana
Authors: Blankson Emmanuel R. , Tetteh Patricia Nakie , Oppong Prince , Gbogbo Francis
Published: February 2022

Urban riverine systems serve as conduits for the transport of plastic waste from the terrestrial dumpsites to marine repositories. This study presented data on the occurrence of microplastics in water, sediment, Bagrid Catfish (Chrysichthys nigrodigitatus) and Black-chinned Tilapia (Sarotherodon melanotheron) from the Densu River, an urban riverine system in Ghana. Microplastics were extracted from the samples collected from both the lentic and lotic sections of the river. The results indicated widespread pollution of the Densu River with microplastics in all the compartments studied. The average numbers of microplastic particles deposited in the Dam (2.0 ± 0.58) and Delta (2.50 ± 0.48) sections of the river were not affected by the differences in their hydrology. However, the stagnant water system of the Dam promoted the floating of larger-sized microplastics while the flowing waters of the Delta did not show any selectivity in the deposition of microplastics between sediment and the water column. The number of microplastics ingestions by the Bagrid Catfish (2.88 ± 2.11) was similar to the Black-chinned Tilapia (2.38 ± 1.66) but both species ingested lower numbers of microplastics than reported for marine fish species in coastal Ghana.
Polypyrimidine tract binding proteins PTBP1 and PTBP2 interact with distinct proteins under splicing conditions
Authors: Pina Jeffrey M. , Hernandez Luis A. , Keppetipola Niroshika M.
Published: February 2022

RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Polypyrimidine tract binding proteins, PTBP1 and PTBP2, are paralogous RNA binding proteins that play a critical role in the process of neuronal differentiation and maturation; changes in the concentration of PTBP proteins during neuronal development direct splicing changes in many transcripts that code for proteins critical for neuronal differentiation. How the two related proteins regulate different sets of neuronal exons is unclear. The distinct splicing activities of PTBP1 and PTBP2 can be recapitulated in an in vitro splicing system with the differentially regulated N1 exon of the c-src pre-mRNA. Here, we conducted experiments under these in vitro splicing conditions to identify PTBP1 and PTBP2 interacting partner proteins. Our results highlight that both PTBPs interact with proteins that participate in chromatin remodeling and transcription regulation. Our data reveal that PTBP1 interacts with many proteins involved in mRNA processing including splicing regulation while PTBP2 does not. Our results also highlight enzymes that can serve as potential “writers” and “erasers” in adding chemical modifications to the PTB proteins. Overall, our study highlights important differences in protein-protein interactions between the PTBP proteins under splicing conditions and supports a role for post-translational modifications in dictating their distinct splicing activities.
The rates and measurement of adherence to acamprosate in randomised controlled clinical trials: A systematic review
Authors: Donoghue Kim , Hermann Laura , Brobbin Eileen , Drummond Colin
Published: February 2022

Aim

The current research aims to systematically review the rates of adherence reported in randomised controlled clinical trials of acamprosate. It also sought to determine the reliability of the adherence monitoring and measurement methods used in these trials.

Methods

The protocol for this review was pre-registered (PROSPERO: CRD42021230011). A search of the literature was conducted using OVID MEDLINE, Embase and PsycINFO from database inception to January 2021. Randomised controlled trials with a minimum sample size of 10 per treatment arm that compared the efficacy of acamprosate with placebo or other active medication in adults with a diagnosis of alcohol dependence were included. Data on rates of adherence, methods of measurement and monitoring of adherence was extracted from eligible studies independently in duplicate by two reviewers. A weighted mean adherence rate was calculated. The reliability of adherence monitoring methods was determined by calculating an adherence-assurance score based on the adherence monitoring method used. Risk of bias was assessed using the Cochrane Risk of Bias Tool.

Results

Fifteen studies met the eligibility criteria involving 4,450 participants (2,480 participants in the placebo arms). A mean adherence rate of 88% (54.2–95.0%) was reported across studies that reported the percentage of medication taken. A mean adherence rate of 84.9% (56.4–91.3%) was reported for trials that reported the percentage of participants taking more than 80% of medication prescribed. There is low confidence in the methods used to monitor adherence with all clinical trials having a low adherence-assurance rating. Risk of bias was judged to be high for all included studies.

Conclusions

Adherence to acamprosate in clinical trials can be poor with low confidence in the methods used to measure it. Adherence rates therefore might not be accurate, which has implications for determining the efficacy of acamprosate.
A brief child-friendly reward task reliably activates the ventral striatum in two samples of socioeconomically diverse youth
Authors: Peckins Melissa K. , Westerman Heidi B. , Burt S. Alexandra , Murray Laura , Alves Martha , Miller Alison L. , Gearhardt Ashley N. , Klump Kelly L. , Lumeng Julie C. , Hyde Luke W.
Published: February 2022

Adolescence is a period of increased risk-taking behavior, thought to be driven, in part, by heightened reward sensitivity. One challenge of studying reward processing in the field of developmental neuroscience is finding a task that activates reward circuitry, and is short, not too complex, and engaging for youth of a wide variety of ages and socioeconomic backgrounds. In the present study, we tested a brief child-friendly reward task for activating reward circuitry in two independent samples of youth ages 7–19 years old enriched for poverty (study 1:n= 464; study 2:n= 27). The reward task robustly activated the ventral striatum, with activation decreasing from early to mid-adolescence and increasing from mid- to late adolescence in response to reward. This response did not vary by gender, pubertal development, or income-to-needs ratio, making the task applicable for a wide variety of populations. Additionally, ventral striatum activation to the task did not differ between youth who did and did not expect to receive a prize at the end of the task, indicating that an outcome of points alone may be enough to engage reward circuitry. Thus, this reward task is effective for studying reward processing in youth from different socioeconomic backgrounds.
Reduced blood pressure in sickle cell disease is associated with decreased angiotensin converting enzyme (ACE) activity and is not modulated by ACE inhibition
Authors: Brito Pamela L. , dos Santos Alisson F. , Chweih Hanan , Favero Maria E. , Gotardo Erica M. F. , Silva Juliete A. F. , Leonardo Flavia C. , Franco-Penteado Carla F. , de Oliveira Mariana G. , Ferreira Wilson A. , Zaidan Bruna C. , Billis Athanase , Baldanzi Giorgio , Mashima Denise A. , Antunes Edson , Saad Sara T. Olalla , Costa Fernando F. , Conran Nicola
Published: February 2022

Background

Sickle cell disease (SCD) incurs vaso-occlusive episodes and organ damage, including nephropathy. Despite displaying characteristics of vascular dysfunction, SCD patients tend to present relatively lower systemic blood pressure (BP), via an unknown mechanism. We investigated associations between BP and renin-angiotensin-system (RAS) components in SCD and determined whether an inhibitor of angiotensin converting enzyme (ACE; often used to slow SCD glomerulopathy) further modulates BP and RAS components in a murine model of SCD.

Methods

BP was compared in human subjects and mice with/without SCD. Plasma angiotensin II, ACE and renin were measured by immunoassay. BP was reevaluated after treating mice with enalapril (25 mg/kg, 5x/week) for 5 weeks; plasma and organs were stored for angiotensin II and ACE activity measurement, and quantitative real-time PCR.

Results

Diastolic BP and systolic BP were significantly lower in patients and mice with SCD, respectively, compared to controls. Reduced BP was associated with increased plasma renin and markers of kidney damage (mice) in SCD, as well as significantly decreased plasma ACE concentrations and ACE enzyme activity. As expected, enalapril administration lowered BP, plasma angiotensin II and organ ACE activity in control mice. In contrast, enalapril did not further reduce BP or organ ACE activity in SCD mice; however, plasma angiotensin II and renin levels were found to be significantly higher in enalapril-treated SCD mice than those of treated control mice.

Conclusion

Relative hypotension was confirmed in a murine model of SCD, in association with decreased ACE concentrations in both human and murine disease. Given that ACE inhibition has an accepted role in decreasing BP, further studies should investigate mechanisms by which ACE depletion, via both Ang II-dependent and alternative pathways, could contribute to reduce BP in SCD and understand how ACE inhibition confers Ang II-independent benefits on kidney function in SCD.
Longitudinal increase in albumin–bilirubin score is associated with non-malignancy-related mortality and quality of life in patients with liver cirrhosis
Authors: Sakamaki Akira , Takamura Masaaki , Sakai Norihiro , Watanabe Yusuke , Arao Yoshihisa , Kimura Naruhiro , Setsu Toru , Abe Hiroyuki , Yokoo Takeshi , Kamimura Hiroteru , Tsubata Shunsuke , Waguri Nobuo , Ishikawa Toru , Kawai Hirokazu , Sugitani Soichi , Sato Tomomi , Funakoshi Kazuhiro , Watanabe Masashi , Igarashi Kentarou , Kamimura Kenya , Tsuchiya Atsunori , Aoyagi Yutaka , Terai Shuji
Published: February 2022

Due to the developments in the treatment for hepatitis, it is possible to prevent the progression of liver fibrosis and improve patients’ prognosis even if it has already led to liver cirrhosis (LC). Consequently, a two-step study was conducted. To begin with, a retrospective study was conducted to identify the potential predictors of non-malignancy-related mortality from LC. Then, we prospectively analyzed the validity of these parameters as well as their association with patients’ quality of life. In the retrospective study, 89 cases were included, and the multivariate Cox regression analysis indicated that age (P = 0.012), model for end-stage liver disease (MELD) score (P = 0.012), and annual rate of change of the albumin–bilirubin (ALBI) score (P < 0.001) were significantly associated with LC prognosis. In the prospective study, 70 patients were included, and the patients were divided into cirrhosis progression and non-progression groups. The univariate logistic regression analysis indicated the serum procollagen type III N-terminal peptide level (P = 0.040) and MELD score (P = 0.010) were significantly associated with the annual rate of change of the ALBI score. Furthermore, the mean Chronic Liver Disease Questionnaire score worsened from 5.3 to 4.9 in the cirrhosis progression group (P = 0.034). In conclusion, a longitudinal increase in the ALBI score is closely associated with non-malignancy-related mortality and quality of life.
Epigenetic clock analysis and increased plasminogen activator inhibitor-1 in high-functioning autism spectrum disorder
Authors: Okazaki Satoshi , Kimura Ryo , Otsuka Ikuo , Funabiki Yasuko , Murai Toshiya , Hishimoto Akitoyo
Published: February 2022

Background

Autism spectrum disorder (ASD) is characterized by impaired social communication and behavioral problems. An increased risk of premature mortality has been observed in individuals with ASD. Therefore, we hypothesized that biological aging is accelerated in individuals with ASD. Recently, several studies have established genome-wide DNA methylation (DNAm) profiles as ‘epigenetic clocks’ that can estimate biological aging. In addition, ASD has been associated with differential DNAm patterns.

Methods

We used two independent datasets from blood samples consisting of adult patients with high-functioning ASD and controls: the 1st cohort (38 ASD cases and 31 controls) and the 2nd cohort (6 ASD cases and 10 controls). We explored well-studied epigenetic clocks such as HorvathAge, HannumAge, SkinBloodAge, PhenoAge, GrimAge, and DNAm-based telomere length (DNAmTL). In addition, we investigated seven DNAm-based age-related plasma proteins, including plasminogen activator inhibitor-1 (PAI-1), and smoking status, which are the components of GrimAge.

Results

Compared to controls, individuals with ASD in the 1st cohort, but not in the 2nd cohort, exhibited a trend for increased GrimAge acceleration and a significant increase of PAI-1 levels. A meta-analysis showed significantly increased PAI-1 levels in individuals with ASD compared to controls.

Conclusion

Our findings suggest there is no epigenetic age acceleration in the blood of individuals with ASD. However, this study provides novel evidence regarding increased plasma PAI-1 levels in individuals with high-functioning ASD. These findings suggest PAI-1 may be a biomarker for high-functioning ASD, however, larger studies based on epigenetic clocks and PAI-1 will be necessary to confirm these findings.
Food habits and associated risk factors of depressed patients with cardiovascular disease
Authors: Aljuhani Hind E. , Alshammari Ghedeir M. , AlHadi Ahmad N. , Alabdulkarem Kholoud B. , Albader Omar Sulaiman M. , Baig Mirza B. , Yahya Mohammed Abdo
Published: February 2022

The present study was carried out to investigate food habits and associated risk factors of depressed patients with cardiovascular disease in Riyadh city, Saudi Arabia. Depressed and healthy females (n = 30 each) and males (n = 30 each) aged 18–65 years were involved in this study. Sociodemographic, anthropometric proxies, and nutritional status were evaluated. Cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-c), and low-density lipoprotein cholesterol (LDL-c) levels of respondents’ blood were determined. The respondents were varied according to demographic factors and anthropometric proxies. The majority of depressed males had higher values than healthy ones. The student t-test analysis showed that the average daily intake of fat especially saturated fat, by depressed respondents was higher than that of the healthy ones as well as the dietary requirement intake (DRI). The analysis of respondents’ blood showed that the number of depressed females had higher abnormal HDL-c than males, who were observed to have an abnormal level of cholesterol and triglycerides. The correlation of daily nutrient intake and depression duration, depression severity, and age showed that the nutrients responsible for the extension and severity of depression were intake of food rich in dietary fat. Factors including demographics daily nutrient intake appeared to be associated with depression.
Prevalence of biofilms in Candida spp. bloodstream infections: A meta-analysis
Authors: Atiencia-Carrera María Belén , Cabezas-Mera Fausto Sebastián , Tejera Eduardo , Machado António
Published: February 2022

Context

Candida-related infections are nowadays a serious Public Health Problem emerging multidrug-resistant strains. Candida biofilm also leads bloodstream infections to invasive systemic infections.

Objective

The present meta-analysis aimed to analyze Candida biofilm rate, type, and antifungal resistance among hospitalized patients between 1995 and 2020.

Data sources

Web of Science, Scopus, PubMed, and Google Scholar databases were searched for English papers using the following medical subject heading terms (MESH): “invasive candidiasis”; “bloodstream infections”; “biofilm formation”; “biofilm-related infections”; “mortality”; and “prevalence”.

Study selection

The major inclusion criteria included reporting the rate of biofilm formation and the prevalence of biofilm-related to Candida species, including observational studies (more exactly, cohort, retrospective, and case-control studies). Furthermore, data regarding the mortality rate, the geographical location of the study set, and the use of anti-fungal agents in clinical isolates were also extracted from the studies.

Data extraction

Independent extraction of articles by 2 authors using predefined data fields, including study quality indicators.

Data synthesis

A total of 31 studies from publicly available databases met our inclusion criteria. The biofilm formation in the data set varied greatly from 16 to 100% in blood samples. Most of the studies belonged to Europe (17/31) and Asia (9/31). Forest plot showed a pooled rate of biofilm formation of 80.0% (CI: 67–90), with high heterogeneity (Q = 2567.45, I2 = 98.83, τ2 = 0.150) in random effects model (p < 0.001). The funnel plot and Egger’s linear regression test failed to find publication bias (p = 0.896). The mortality rate in Candida-related bloodstream infections was 37.9% of which 70.0% were from biofilm-associated infections. Furthermore, Candida isolates were also characterized in low, intermediate, or high biofilm formers through their level of biofilm mass (crystal violet staining or XTT assays) after a 24h growth. When comparing between countries, statistical differences were obtained (p = 0.0074), showing the lower and higher biofilm prevalence values in Italy and Spain, respectively. The prevalence of low, intermediate, and high biofilms were 36.2, 18.9, and 35.0% (p < 0.0001), respectively. C. tropicalis was the prevalent species in high biofilm formation (67.5%) showing statistically significant differences when compared to other Candida species, except for C. krusei and C. glabrata. Finally, the rates of antifungal resistance to fluconazole, voriconazole, and caspofungin related to biofilm were 70.5, 67.9 and 72.8% (p < 0.001), respectively.

Conclusions

Early detection of biofilms and a better characterization of Candida spp. bloodstream infections should be considered, which eventually will help preserve public health resources and ultimately diminish mortality among patients.
Phantoms of silenced pasts: Lessons for today from the forgotten history of Burmese Indians’ exodus from Burma
Authors: Bahukhandi Annima
Published: February 2022

This article explores the lives of Burmese Indians – Indian minorities that had migrated to and subsequently settled in Colonial Burma but later repatriated or fled Burma between the 1930s and 1960s. The stories of Burmese Indians who once made up a noticeable minority in Burma are conspicuously under-represented from historical records of both India and Burma today. Due to this silencing, these stories have receded to the periphery of public memory and now survive as grandparents’ tales of immigration and folk memories. Furthermore, the invisibilization of these stories from the Burmese memory-scape has created a peculiar situation wherein not only is the Burmese Indians’ exodus disembedded from Burma’s wider history but the current ethnic tensions, particularly the violence against the Rohingya Muslims, is perceived as delinked from any anti-India rhetoric of the past. This article attempts to weave the Burmese Indians’ exodus into the larger historical trajectory of the state and read the Rohingya Muslims’ exodus as another illustration of Burma’s haunting legacy of dealing with the ethnically different ‘other’.
‘Being the culture’ and ‘playing the culture’: Choro and the Brazilianness performed in Brussels
Authors: Barbosa Eliana Rosa de Queiroz
Published: February 2022

Choro is an instrumental Brazilian music genre that emerged in Rio de Janeiro in the nineteenth century. It can adopt various forms with a basis of guitar, flute and cavaquinho – the latter, a small guitar of Portuguese origin. Musical arrangements are normally elaborate, although in Brazil the players gather in usually informal and open settings, in indoors or outdoors formations called rodas (‘circles’). Following the customs of many Brazilian genres, players sit in a circle facing each other, the audience stands around and, occasionally, skilled couples dance to it. A roda de choro is not a concert, yet it is not just a rehearsal, but also a musical experience based on spontaneity. This article, drawing upon an observation exercise in the Brussels (Belgium) choro scene, intends to explore the multitude of meanings of choro as a practice in which Brazilians in diaspora, other migrants and locals engage and share experiences with each other, focusing on the social geographies of choro and the social networks derived from this musical practice. The observation of this music-making process in Brussels raises additional questions of Brazilian (musical) identity in diaspora and its relation to the notions of longing (saudades), authenticity, affinity, transcendence and joy.