bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISIS HUBUNGAN TINGKAT KEBAHAGIAAN DENGAN KUALITAS LINGKUNGAN DAN PEMBANGUNAN TEKNOLOGI INFORMASI
Authors: Rusyda Muhammad , Siagian Tiodora Hadumaon
Published: June 2023

Pencemaran lingkungan merupakan persoalan yang menjadi prioritas untuk dicegah dan diatasi. Pengelolaan lingkungan harus mampu menjaga keseimbangan lingkungan agar kondisi lingkungan dapat terpelihara dengan baik. Lingkungan yang sehat akan memberikan kenyamanan dalam kehidupan masyarakat. Di sisi lain, pembangunan teknologi informasi dan komunikasi (TIK) yang pesat memberikan berbagai kemudahan dalam berkomunikasi, memperoleh informasi dan akses terhadap hiburan. Akan tetapi, kemudahan tersebut bisa memberikan pengaruh negatif seperti berkurangnya interaksi sosial, meningkatnya kejahatan berbasis teknologi informasi, dan sebagainya. Pertanyaannya adalah apakah benar lingkungan hidup yang lebih sehat dan pesatnya pembangunan TIK menjadikan masyarakat lebih bahagia? Penelitian ini mencoba menganalisis hal tersebut menggunakan data panel 34 provinsi di Indonesia dengan metode regresi data panel. Hasil penelitian menunjukkan bahwa kualitas lingkungan hidup dan pembangunan TIK berpengaruh positif terhadap kebahagiaan. Hasil ini memberikan kesimpulan bahwa untuk meningkatkan kebahagiaan, dapat dilakukan dengan terus menjaga kualitas lingkungan hidup, seperti mencegah dan mengatasi pencemaran udara, air dan tanah. Kemudian sisi positif dari teknologi informasi dan komunikasi perlu terus ditingkatkan sehingga dapat memberikan manfaat yang lebih luas bagi masyarakat.
Інформаційне суспільство: проблеми та перспективи : матеріали VІІІ Всеукр. наук.-практич. конф. (м. Одеса, 2 черв. 2023 р.)
Authors:   Національний університет «Одеська юридична академія»
Published: June 2023

Всеукраїнська науково-практична конференція «Інформаційне суспільство: проблеми та перспективи» присвячена розгляду актуальних питань розвитку інформаційного суспільства в Україні. До участі у конференції запрошені студенти, аспіранти, молоді вчені, викладачі та науковці. Конференція проводиться на базі Національного університету «Одеська юридична академія».
GÖNÜLLÜ GERİ DÖNÜŞ
Authors: TARMAN Zeynep Derya
Published: June 2023

Göç hareketlerinin sebep olduğu siyasi, ekonomik ve sosyal sorunlar ev sahibi devletlerin göç yönetiminde kalıcı ve uzun vadeli çözüm arayışlarını beraberinde getirmiştir. Bu kapsamda bir yandan kişilerin menşe ülkelerine geri dönme hakkına diğer yandan devletlerin kendi yükümlülüklerini sınırlama yolundaki eğilimlerine hizmet eden gönüllü geri dönüş devletler tarafından en çok desteklenen çözüm yolu olarak karşımıza çıkmaktadır. Bu çalışmada gönüllü geri dönüş kavramı öncelikle uluslararası hukuk kaynakları çerçevesinde incelenmiş, ardından Avrupa Birliği politikaları ile Belçika, İsviçre, Birleşik Krallık ve Norveç olmak üzere başlıca Avrupa ülkelerindeki gönüllü geri dönüşe ilişkin örnek uygulamalar açıklanmıştır. Bunu takiben Türk mevzuatı ve uygulamasında gönüllü geri dönüş mekanizması ele alınmıştır. Türk hukukunda gönüllü geri dönüşe ilişkin temelde uluslararası hukukta öngörülen esasları içeren hükümlere yer verilmiştir. Ancak Türkiye’nin ev sahipliği yaptığı göçmen sayısı ve etkili bir göç yönetiminde gönüllü geri dönüşün önemi göz önüne alındığında gönüllü geri dönüşe özgülenmiş bir mevzuata ihtiyaç olduğu anlaşılmaktadır. Bu kapsamda ideal gönüllü geri dönüş mekanizmasında bulunması gereken unsurlar değerlendirilmiştir. Gönüllü geri dönüşü temin etmek için geri döneceklere finansal destek sağlandığı ve sürdürülebilir yardımlı gönüllü geri dönüş programları faaliyete geçirildiği gözlemlenmektedir. Sonuç olarak, Türkiye’ye yönelik özellikle son yıllarda artan düzenli ve düzensiz göç hareketi gözetilerek gönüllü geri dönüş kavramının ikincil bir mevzuatla daha detaylı biçimde yasal temeline kavuşturulması planlı, ekonomik ve etkin bir göç yönetimi için önem arz etmektedir.
Dampak Pandemi Covid-19 terhadap Perekonomian Indonesia
Authors: Ramadhani Yesa Cahayaning
Published: June 2023

The purpose of this research is to analyze the impact of the Indonesian economy during the Covid-19 pandemic. The population in this study is the economy in Indonesia caused by the Covid-19 pandemic. The determination of the sample was carried out using a purposive sampling technique, based on certain criteria in 2019–2020. The data source used is secondary data obtained in the form of observations of the economy in Indonesia which has been affected by the Covid-19 pandemic (online). Testing the research hypothesis using the SEM analysis method (structural equation model). The first results obtained show that inflation and the unemployment rate have proven to have a significant positive effect on the Indonesian economy; while the Covid-19 pandemic has had a positive but unsignificant effect on the Indonesian economy. The second result found that inflation proved to have a positive but unsignificant effect on national investment; on the other hand, the unemployment rate and the Covid-19 pandemic proved to have a positive and significant effect on national investment.
Sistem Pendukung Keputusan Pemilihan Penayangan Iklan Radio Menggunakan Metode Weighted Product (WP)
Authors: Yunitarini Rika
Published: June 2023

Penayangan program radio yang diselingi iklan membutuhkan pengelolaan yang baik sehingga dapat tepat sasaran. Seperti jam atau waktu kemunculan iklan tersebut, lama penayangan atau durasi, dan seberapa sering iklan itu muncul di sela program-program radio yang disiarkan. Masalah yang sering terjadi adalah kesulitan dalam memilih mana dari sekian banyak iklan yang akan ditayangan oleh stasiun radio tersebut. Pemilihan ini didasarkan pada beberapa kriteria seperti kesesuaian dengan visi misi radio, jangka kontrak, harga, durasi tayang, serta jam tayang. Selain itu permasalahan lainnya adalah bagaimana membagi penayangan jenis iklan yang beragam, menentukan waktu tayang, pembagian jam tayang prime time dan reguler time. Rumitnya cara pemilihan penayangan iklan sebagai alasan diusulkanya suatu sistem pendukung keputusan yang dapat menentukan iklan yang akan ditayangkan, dengan kriteria yang memenuhi pihak stasiun radio. Dalam sistem ini menggunakan metode Weighted Product. Metode Weighted Product merupakan metode yang mendukung pengambilan keputusan terhadap multi kriteria. Dengan metode ini diharapkan dapat membantu proses pengambilan keputusan pemilihan penayangan iklan di radio.
Índices de conforto térmico em aves de produção – Revisão sistemática
Authors: Calderaro Cristhiano Ferreira , Brennecke Kathery , Pereira Luiz Arthur Malta , Adorno Leticia Salomão Barbosa , Martins Otto Oliveira , Lima Tamires de Oliveira , Sgavioli Sarah
Published: June 2023

Com base na importância do tema, este trabalho propôs realizar uma revisão sistemática dos trabalhos publicados envolvendo variáveis biometereológicas e índices de conforto térmico para as aves de produção (frangos de corte, poedeiras comerciais e matrizes). Esta revisão sistemática foi realizada por meio de pesquisa e seleção de artigos completos, publicados em periódicos científicos, em três bancos de dados digitais (Scopus, Scielo e PubMed). Os critérios utilizados para a seleção dos artigos foram publicações em periódicos nacionais e internacionais e artigos com dados sobre desempenho e variáveis biometereológicas ou índices de conforto térmico. Na determinação do banco de dados criados desta revisão, a categoria frango de corte contribuiu com 64,29% dos trabalhos, poedeiras comerciais com 33,93% dos artigos e matrizes com 1,78%. O parâmetro temperatura dentre as variáveis biometereológicas foi avaliado em 100% das publicações de frangos de corte e poedeiras comerciais, no entanto, do total de 56 artigos que entraram para a revisão sistemática, apenas 25,00% calcularam índices de conforto térmico. Conclui-se com base na revisão sistemática, que apesar da crescente evolução da publicação de trabalhos envolvendo os índices de conforto térmico em aves, este tema ainda requer atenção dos pesquisadores, com o objetivo de desenvolver técnicas que favoreçam o conforto térmico de aves de produção.
A contribuição da análise do comportamento aplicada – ABA na inclusão de crianças com transtorno do espectro autista no âmbito escolar
Authors: Felinto Jislayne Fidelis , Barbosa Tatiane Vanessa dos Santos , Ferreira Marta Betânia Lopes Soares , Dutra Adriana Bastos de Oliveira , Gomes Maria Saturnino de Figueiredo , Fidelis Jéssica da Silva , Silva Maria Juscilene Nestor da , Drieskens Davi Carvalho
Published: June 2023

Este artigo teve como objetivo - compreender a contribuição da ABA na inclusão de crianças com TEA no ambiente escolar. O estudo em questão é de natureza qualitativa, do tipo, bibliográfica – narrativa, onde buscou-se realizar um levantamento bibliográfico na ferramenta digital - Google, tendo como descritores de busca – a importância da ABA na escola ou a contribuição da ABA na inclusão dos alunos na escola. Posteriormente, foi considerado apenas as pesquisas que tinham nos títulos as informações compatíveis com objetivo desta pesquisa. Com isso, foi possível reunir oito pesquisas, sendo seis artigos e dois Trabalhos de Conclusão de Curso. Então, chegou-se à conclusão, por meio desse levantamento que, as pesquisas encontradas reforçam sobre a contribuição da ABA no ambiente educacional, ao qual vem gerando inclusão. Essa contribuição está relacionada com o desenvolvimento da autonomia e o desenvolvimento cognitivo da criança autista, como também, da sua participação social. Para tais benefícios, são necessárias mudanças nas estratégias pedagógicas, como a formação continuada dos professores em ABA, currículo adaptado e um trabalho em conjunto e bem articulado com todos os profissionais envolvidos no processo de aprendizagem da criança.
O SOTER-PA como ferramenta de apoio a gestão sustentável da vegetação remanescente em unidades de manejo florestal
Authors: Rocha Bianca Diniz da , Roque Mariane Paulina Batalha , Moreira Mayron César de Oliveira , Ferreira Neto José Ambrósio
Published: June 2023

Este trabalho avalia a eficiência do sistema informatizado SOTER-PA no processo de organização territorial e a priorização de áreas para conservação em Unidades de Manejo Florestal, com base nas espécies, distribuição e importância. O SOTER-PA é um aplicativo construído em linguagem de programação Java baseado no algoritmo busca tabu minimalista, com o qual o usuário pode obter uma estrutura de planejamento territorial mais rentável economicamente, com melhor uso dos recursos naturais e garantia dos futuros ciclos de cortes. Como resultado, constatou-se que o uso do SOTER-PA apresenta limitações em processar áreas complexas e com muitas variáveis, exigindo maior tempo de processamento, além de equipamentos com capacidade de processamento superior ao que geralmente se encontra em condições reais de uso. Todavia, quando aplicado a áreas menores, mostrou-se uma alternativa eficiente para o planejamento florestal com garantia de manutenção da diversidade e a conservação da floresta, demonstrando ser uma boa ferramenta para o manejo florestal sustentável.
Análise Situacional da Gestão Estratégica das Incubadoras Tecnológicas de Cooperativas Populares na Paraíba
Authors: Moraes Elenice da Silva , Borba Marcelo da Costa , Ramos Josefa Edileide Santos , Costa José Marcione da , Lima Telma Lúcia Andrade de
Published: June 2023

As Incubadoras Tecnológicas de Cooperativas Populares (ITCP) são grandes promotoras do desenvolvimento de inovações tecnológicas nos setores carentes da economia. Este trabalho tem como objetivo realizar um diagnóstico situacional das ITCPs na Paraíba. Trata-se de uma pesquisa qualitativa, classificando-se, quanto aos objetivos, como descritiva; quanto aos meios, como pesquisa de campo. O tratamento dos dados coletados nas entrevistas semiestruturadas foi feito pelo método de análise de conteúdo. As entrevistas diagnosticaram que as ITCPs entrevistadas são grandes apoiadoras do desenvolvimento de empreendimentos econômicos solidários, pois desenvolvem vários empreendimentos em diferentes ramos de atividade, mesmo com dificuldades de apoio e financiamento. Diante disso, os resultados obtidos no estudo mostraram que a gestão estratégica das ITCPs é importante e permite que os empreendimentos incubados sejam eficientes e sustentáveis em seus negócios.
Obesidade, diabetes e COVID-19: a morbimortalidade em um hospital particular do oeste do Paraná
Authors: Fritzen Camila Hoffmann , Pescador Marise Vilas Boas , Ferreira Dhanylo Dener , Brandão Bruno Meloti , Hamdan Mariam Walid , Santos Leonardo Rafael Prado dos , Nascimento Rodrigo Araújo , Freitas Pedro Nascimento Miranda , Coelho Isabela Raupp , Lima Nathana Massucatto
Published: June 2023

Objetivo: analisar quantitativa e qualitativamente os pacientes internados por COVID-19 no Hospital São Lucas, da cidade Cascavel – PR, correlacionando o prognóstico desses pacientes com morbidades preexistes como obesidade e diabetes mellitus. Métodos: tratou-se de uma pesquisa exploratória transversal, seccional, retrospectiva, quantitativa e qualitativa de coleta de dados de janeiro a junho de 2021. Foram utilizados dados de prontuários de 385 pacientes, internados por COVID-19 e atendidos no Hospital São Lucas. Resultados: nos 385 pacientes analisados, 66% eram do sexo masculino e 87,4% tinham idade superior a 41 anos. Os diabéticos foram 16,9% da amostra. O IMC indicou sobrepeso ou obesidade em 74,9%. A maioria permaneceu hospitalizada de 4 a 14 dias. Sobre a análise radiológica, 91,3% apresentou comprometimento pulmonar, o qual foi de 26% a 75% do parênquima em 80,61%. Assistência respiratória foi demandada por 94% - na forma de ventilação mecânica não invasiva para 56,9%, e invasiva para 37,1. A taxa de mortalidade foi de 27,5% - sendo idade, sexo masculino e demanda por ventilação mecânica invasiva seus fatores de risco mais evidentes. Conclusão: Idade, sexo masculino e necessidade de ventilação mecânica invasiva foram os fatores de pior prognóstico frente à COVID-19. O diabetes mellitus foi associado a maior comprometimento pulmonar. O IMC se mostrou não relevante na análise dos desfechos, possivelmente pela grande quantidade de pacientes na amostra com sobrepeso ou obesidade.
The action of the pharmacist in front of bacterial resistance and rational use of antimicrobials in the hospital environment
Authors: Lima Brenda Caroline de Araújo , Silva Emily dos Santos , Jesus Robson de , Abreu Emilly Anny Benevides de , França Fabiana Chagas Oliveira de , Santos Aranin Queiroz de Sousa , Alkmin José Alfredo de Oliveira , Barbosa Anderson dos Santos
Published: June 2023

Antimicrobials are one of the best discoveries of the 20th century, which concomitant with their use came one of the biggest current problems in the world, bacterial resistance. Within this context, the present study aims to highlight the role of the pharmacist in the face of bacterial resistance and rational use of antimicrobials in the hospital environment. For the development of the study, a literature review was carried out for a reflective analysis based on studies published from 2010 to 2022 in the main databases found in Portuguese, English and Spanish. We selected 20 articles out of 6,650 found before applying the inclusion and exclusion criteria and performing the analysis. The clinical pharmacist plays a key role in combating bacterial resistance. In addition, the hospital environment is a place where there are more people in the same environment administering various antibiotics and different spectrums. The pharmaceutical professional has a direct and indirect impact on patients and medicines, being responsible for promoting the rational use of antibiotics, with standardization of medicines and possible interventions in the treatment, providing care, insurance, and health to the patient. Therefore, it is an important key in combating bacterial resistance, which is a public health issue, in addition to the possibility of promoting research and innovation in the field of microbiology and antimicrobial therapy.
Acolhimento de adolescentes com ideações e tentativas suicidas
Authors: Saraiva Lucélia Sousa Martins , Silva Rivania Vieira da , Alves Larissa Luz
Published: June 2023

Este artigo é uma revisão integrativa da literatura que tem como objetivo investigar as principais estratégias de prevenção do suicídio entre jovens. A busca foi realizada em bases de dados eletrônicas e foram incluídos estudos que abordavam estratégias de prevenção do suicídio em diferentes populações jovens, incluindo LGBTQ+, indígenas e universitários. A análise dos resultados mostrou que as principais estratégias incluem a promoção da saúde mental, o acesso a serviços de saúde mental sensíveis à diversidade, a criação de ambientes seguros e inclusivos, a colaboração entre os sistemas de saúde e educação, o envolvimento das famílias e comunidades, a abordagem multidisciplinar e a promoção da cultura e tradições indígenas. As considerações finais destacam a importância de abordagens personalizadas, adaptadas às necessidades específicas de cada grupo, e da realização de mais estudos para aprimorar as estratégias de prevenção do suicídio entre jovens. As três palavras-chave são: suicídio, prevenção, jovens.
Detecção de câncer de mama: avanços e desafios
Authors: Almeida Hernesto Vaz , Correia Americo Costa , Pacheco Ismar de Jesus , Ferreira Marllon Frank Teixeira , Silva Jamylly Dias , Gomes Lusenilde Pereira , Souza Maria Tereza Pereira de
Published: June 2023

Objetivo: Fornecer uma visão geral dos métodos de detecção de câncer de mama, os desafios enfrentados e as perspectivas futuras na detecção precoce dessa patologia. Metodologia: Este estudo enquadra-se como uma revisão narrativa da literatura, de caráter qualitativo exploratório. Resultados e Discussão: A mamografia é o método mais utilizado e comprovadamente eficaz na detecção precoce do câncer de mama, especialmente em mulheres de faixa etária mais avançada. Além desse método, avanços recentes na tecnologia médica têm levado ao desenvolvimento de outras técnicas de detecção de câncer de mama, como a ressonância magnética da mama, a ultrassonografia e a tomossíntese mamária. Conclusão: A detecção precoce do câncer de mama é de extrema importância para aumentar as chances de cura e melhorar os resultados do tratamento. A combinação de diferentes métodos de detecção e o acompanhamento regular com profissionais de saúde são fundamentais para garantir uma detecção precoce e um tratamento adequado do câncer de mama.
O uso de preenchedores e técnicas de escultura labial em fissuras labiais: uma revisão narrativa da literatura
Authors: Nardi Bianca Maria Fabrízio , Suguihara Roberto Teruo , Muknicka Daniella Pilon
Published: June 2023

As fissuras labiais e palatinas são anomalias congênitas resultantes de uma falha na fusão dos processos labiais e palatinos durante o desenvolvimento embrionário. Os lábios desempenham um papel crucial na estética facial, expressando sentimentos, beleza e atratividade. Para pessoas que passaram por cirurgia reparadora de lábio leporino, ter lábios bem desenhados e proporcionais à face é essencial para transmitir não apenas beleza, mas também saúde emocional. Embora o reparo cirúrgico das fissuras labiais seja fundamental para corrigir a deformidade e a disfunção, podem ocorrer imperfeições cosméticas residuais, como cicatrizes e queloides, que são comuns nesses pacientes. A assimetria resultante e o baixo volume do lábio superior podem ser tratados cirurgicamente ou por meio de abordagens menos invasivas. O uso de preenchimento temporário de tecido mole, como o ácido hialurônico, tem se mostrado uma alternativa para corrigir a assimetria labial característica dessas malformações e melhorar o volume após a reparação cirúrgica. Além disso, no tratamento para reduzir a aparência das cicatrizes, a subincisão e o microagulhamento são técnicas propostas, sendo que a combinação das duas tem demonstrado melhores resultados. Dessa forma, o objetivo desse trabalho é realizar uma revisão de literatura sobre a abordagem terapêutica das fissuras labiais e palatinas, com foco nas técnicas de reparo cirúrgico e no uso de preenchimento temporário de tecido mole para corrigir imperfeições cosméticas residuais, além de avaliar os tratamentos propostos para reduzir a aparência de cicatrizes, como a subincisão e o microagulhamento.
Loss of TDP-43 causes ectopic endothelial sprouting and migration defects through increased fibronectin, vcam 1 and integrin α4/β1
Authors: Hipke Katrin , Pitter Bettina , Hruscha Alexander , van Bebber Frauke , Modic Miha , Bansal Vikas , Lewandowski Sebastian A. , Orozco Denise , Edbauer Dieter , Bonn Stefan , Haass Christian , Pohl Ulrich , Montanez Eloi , Schmid Bettina
Published: June 2023

Aggregation of the Tar DNA-binding protein of 43 kDa (TDP-43) is a pathological hallmark of amyotrophic lateral sclerosis and frontotemporal dementia and likely contributes to disease by loss of nuclear function. Analysis of TDP-43 function in knockout zebrafish identified an endothelial directional migration and hypersprouting phenotype during development prior lethality. In human umbilical vein cells (HUVEC) the loss of TDP-43 leads to hyperbranching. We identified elevated expression of FIBRONECTIN 1 (FN1), the VASCULAR CELL ADHESION MOLECULE 1 (VCAM1), as well as their receptor INTEGRIN α4β1 (ITGA4B1) in HUVEC cells. Importantly, reducing the levels of ITGA4, FN1, and VCAM1 homologues in the TDP-43 loss-of-function zebrafish rescues the angiogenic defects indicating the conservation of human and zebrafish TDP-43 function during angiogenesis. Our study identifies a novel pathway regulated by TDP-43 important for angiogenesis during development.
Long-term CMV monitoring and chronic rejection in renal transplant recipients
Authors: Ishikawa Shoko , Tasaki Masayuki , Saito Kazuhide , Nakagawa Yuki , Ikeda Masahiro , Takahashi Kota , Tomita Yoshihiko
Published: June 2023

Introduction

Cytomegalovirus (CMV) is well established to be an independent risk factor for graft loss after kidney transplantation (KTx). Monitoring for CMV in the chronic phase is not defined in the current guideline. The effects of CMV infection, including asymptomatic CMV viremia, in the chronic phase are unclear.

Methods

We performed a single-center retrospective study to investigate incidence of CMV infection in the chronic phase, defined as more than 1 year after KTx. We included 205 patients who received KTx between April 2004 and December 2017. The CMV pp65 antigenemia assays to detect CMV viremia were continuously performed every 1–3 months.

Results

The median duration of the follow-up was 80.6 (13.1–172.1) months. Asymptomatic CMV infection and CMV disease were observed in 30.7% and 2.9% in the chronic phase, respectively. We found that 10–20% of patients had CMV infections in each year after KTx which did not change over 10 years. The history of CMV infection in the early phase (within 1 year after KTx) and chronic rejection were significantly associated with CMV viremia in the chronic phase. CMV viremia in the chronic phase was significantly associated with graft loss.

Discussion

This is the first study to examine the incidence of CMV viremia for 10 years post KTx. Preventing latent CMV infection may decrease chronic rejection and graft loss after KTx.
Effects of multi-organ crosstalk on the physiology and pathology of adipose tissue
Authors: Wang Sufen , Liu Yifan , Chen Jiaqi , He Yuejing , Ma Wanrui , Liu Xinguang , Sun Xuerong
Published: June 2023

In previous studies, adipocytes were found to play an important role in regulating whole-body nutrition and energy balance, and are also important in energy metabolism, hormone secretion, and immune regulation. Different adipocytes have different contributions to the body, with white adipocytes primarily storing energy and brown adipocytes producing heat. Recently discovered beige adipocytes, which have characteristics in between white and brown adipocytes, also have the potential to produce heat. Adipocytes interact with other cells in the microenvironment to promote blood vessel growth and immune and neural network interactions. Adipose tissue plays an important role in obesity, metabolic syndrome, and type 2 diabetes. Dysfunction in adipose tissue endocrine and immune regulation can cause and promote the occurrence and development of related diseases. Adipose tissue can also secrete multiple cytokines, which can interact with organs; however, previous studies have not comprehensively summarized the interaction between adipose tissue and other organs. This article reviews the effect of multi-organ crosstalk on the physiology and pathology of adipose tissue, including interactions between the central nervous system, heart, liver, skeletal muscle, and intestines, as well as the mechanisms of adipose tissue in the development of various diseases and its role in disease treatment. It emphasizes the importance of a deeper understanding of these mechanisms for the prevention and treatment of related diseases. Determining these mechanisms has enormous potential for identifying new targets for treating diabetes, metabolic disorders, and cardiovascular diseases.
Algal proliferation risk assessment using Vine Copula-based coupling methods in the South-to-North Water Diversion Project of China
Authors: Nong Xizhi , Zeng Jun , Ma Yonghang , Chen Lihua , Zhang Chi , Behzadian Kourosh , Campos Luiza C.
Published: June 2023

The Middle Route of the South-to-North Water Diversion Project of China (MRSNWDPC), i.e., the longest inter-basin water diversion project (1,432 km) in the world, has delivered more than 60 billion m3 of water resources to North China and benefiting more than 100 million people since December 2014. However, the abnormal algal proliferation in the main canal under low nutrient background has seriously threatened the water quality safety of this mega project. In this research, 3 years of monitoring data matrix, including water temperature (WT), flow discharge (Q), flow velocity (V), dissolved oxygen (DO), and the algal cell density (ACD), from the main canal of the MRSNWDPC were analyzed. The nonlinear relationships were determined based on multiple regression models, and a composite risk analysis model was constructed by Latin hypercube sampling (LHS) method coupled with Vine Copula function. The impacts of different hydrological and environmental factors on algal proliferation were comprehensively analyzed by Bayesian theory. The results showed that the WT gradually decreased from upstream to downstream, with a narrow range of 16.6–17.4°C, and the annual average concentrations of DO showed a gradual increase from upstream to downstream. The flow velocity of MRSNWDPC had a tendency to increase year by year, and the maximum flow velocity exceeds 0.8 m/s upstream, midstream and downstream by 2018. The ACD accumulated along the main canal, and the annual average ACDs of downstream were the highest, ranging from 366.17 to 462.95 × 104 cells/L. The joint early-warning method considering both water temperature and flow velocity conditions is an effective way for algal proliferation risk warning management. When water temperatures of the upstream, midstream, and downstream were below 26, 26, and 23°C, respectively, the algal proliferation risk can be controlled under 50% by the flow velocity at 0.3 m/s; otherwise, the flow velocity needs to be regulated higher than 0.8 m/s. In order to keep the midstream and downstream avoid abnormal algal proliferation events (ACD ≥ 500 × 104 cells/L), the corresponding ACDs of the upstream and midstream need to be controlled lower than 319 × 104 cells/L and 470 × 104 cells/L, respectively. This study provides a scientific reference for the long-distance water diversion project’s algal control and environmental protection. The proposed coupling Vine Copula models can also be widely applied to multivariate risk analysis fields.
A case report of complete remission of acute myeloid leukemia combined with DNMT3A, FLT3-TKD, and IDH2 gene mutations and active pulmonary tuberculosis treated with homeharringtonine + venetoclax + azacytidine
Authors: Ji Lin , Yang Wei , Xu Xiao-feng , Xu Ya-qing
Published: June 2023

In March 2022, a 58-year-old man was admitted to the local hospital for nausea and vomiting. His blood routine indicated that he had leukocytosis and anemia. The patient was diagnosed with acute myeloid leukemia (AML)-M5b accompanied by DNMT3A, FLT3-TKD, and IDH2 mutations, chest CT revealed pulmonary tuberculosis (TB). Acid-fast bacillus (AFB) was detected in sputum. The patient then received anti-TB treatment with isoniazid + rifampicin + pyrazinamide + ethambutol. On April 8, he was transferred to our hospital's Hematology Department after three consecutive negative sputum smears. He was administered the VA (Venetoclax + Azacytidine) regimen of anti-leukemia treatment and also received levofloxacin + isohydrazide + pyrazinamide + ethambutol anti-TB treatment. After one course of VA therapy, there was no remission in the bone marrow. Therefore, the patient received the HVA (Homeharringtonine + Venetoclax + Azacytidine) regimen of anti-leukemia treatment. On May 25, the bone marrow smear revealed that the original mononuclear cells were 1%. Moreover, bone marrow flow cytometry revealed the absence of any abnormal cells. mNGS showed DNMT3A (mutation rate 44.7%), but no mutations were detected in FLT3-TKD and IDH2. The patient then received the HVA regimen three consecutive times, resulting in complete remission. Repeated chest CT examinations revealed progressive regression of pulmonary TB foci, no AFB was detected in the sputum. This AML patient with DNMT3A, FLT3-TKD, and IDH2 mutations and active TB is difficult to treat. It is very necessary for him to administer prompt anti-leukemia treatment under the premise of active anti-TB treatment. The HVA regimen is effective for this patient.
Consequences of scarcity: the impact of perceived scarcity on executive functioning and its neural basis
Authors: Huang Long , Li Xiaojuan , Xu Fuming , Li Fuhong
Published: June 2023

Introduction

Previous studies have found a causal relationship between scarcity and the adverse impact it has on executive functioning. However, few studies have directly examined perceived scarcity, and cognitive flexibility (the third component of executive functions) has rarely been included.

Methods

Using a 2 (group: scarcity group vs. control group) × 2 (trial type: repeat trial vs. switch trial) mixed design, this study directly explored perceived scarcity’s impact on cognitive flexibility and revealed its neural basis in the switching tasks. Seventy college students participated in this study through open recruitment in China. A priming task was used to induce perceived scarcity, thus exploring the impact of perceived scarcity on participants’ performance in switching tasks and enabling the analysis of the neural activity of the brain, combined with electroencephalograph (EEG) technology.

Results

In terms of behavioral outcomes, perceived scarcity led to poorer performance and a greater switching cost of reaction time in the switching tasks. Regarding neural activity, perceived scarcity led to an increase in the amplitude of P3 differential wave (repeat trials minus switch trials) in the parietal cortex during the target-locked epochs in the switching tasks.

Discussion

Perceived scarcity can lead to changes in the neural activity of the brain regions related to executive functioning, resulting in a temporary decrease in cognitive flexibility. It may lead to individuals unable to adapt well to the changing environment, unable to quickly devote themselves to new tasks, and reduce work and learning efficiency in daily life.
Development of a synchronous recording and photo-stimulating electrode in multiple brain neurons
Authors: Zhang Qingping , Jing Wei , Wu Shiping , Zhu Mengzheng , Jiang Jingrui , Liu Xiang , Yu Dian , Cheng Long , Feng Bin , Wen Jianbin , Xiong Feng , Lu Youming , Du Huiyun
Published: June 2023

The investigation of brain networks and neural circuits involves the crucial aspects of observing and modulating neurophysiological activity. Recently, opto-electrodes have emerged as an efficient tool for electrophysiological recording and optogenetic stimulation, which has greatly facilitated the analysis of neural coding. However, implantation and electrode weight control have posed significant challenges in achieving long-term and multi-regional brain recording and stimulation. To address this issue, we have developed a mold and custom-printed circuit board-based opto-electrode. We report successful opto-electrode placement and high-quality electrophysiological recordings from the default mode network (DMN) of the mouse brain. This novel opto-electrode facilitates synchronous recording and stimulation in multiple brain regions and holds promise for advancing future research on neural circuits and networks.
A novel GRN mutation in an Italian patient with non-fluent variant of primary progressive aphasia at onset: a longitudinal case report
Authors: Castelnovo Veronica , Canu Elisa , Domi Teuta , Pozzi Laura , Vignaroli Francesca , Spinelli Edoardo Gioele , Ghirelli Alma , Tondo Giacomo , Comi Cristoforo , Riva Nilo , Quattrini Angelo , Carrera Paola , Filippi Massimo , Agosta Federica
Published: June 2023

Objectives

We report the clinical presentation and evolution of a case with a novel Progranulin gene (GRN) mutation and non-fluent language disturbances at onset.

Materials and methods

A 60 year-old, white patient was followed due to a history of language disturbances. Eighteen months after onset, the patient underwent FDG positron emission tomography (PET), and at month 24 was hospitalized to perform neuropsychological evaluation, brain 3 T MRI, lumbar puncture for cerebrospinal fluid (CSF) analysis, and genotyping. At month 31, the patient repeated the neuropsychological evaluation and brain MRI.

Results

At onset the patient complained prominent language production difficulties, such as effortful speech and anomia. At month 18, FDG-PET showed left fronto-temporal and striatal hypometabolism. At month 24, the neuropsychological evaluation reported prevalent speech and comprehension deficits. Brain MRI reported left fronto-opercular and striatal atrophy, and left frontal periventricular white matter hyperintensities (WMHs). Increased CSF total tau level was observed. Genotyping revealed a new GRN c.1018delC (p.H340TfsX21) mutation. The patient received a diagnosis of non-fluent variant of primary progressive aphasia (nfvPPA). At month 31, language deficits worsened, together with attention and executive functions. The patient presented also with behavioral disturbances, and a progressive atrophy in the left frontal-opercular and temporo-mesial region.

Discussion and conclusion

The new GRN p.H340TfsX21 mutation resulted in a case of nfvPPA characterized by fronto-temporal and striatal alterations, typical frontal asymmetric WMHs, and a fast progression toward a widespread cognitive and behavioral impairment, which reflects a frontotemporal lobar degeneration. Our findings extend the current knowledge of the phenotypic heterogeneity among GRN mutation carriers.
Towards unravelling biological mechanisms behind radiation-induced oral mucositis via mass spectrometry-based proteomics
Authors: Subedi Prabal , Huber Katharina , Sterr Christoph , Dietz Anne , Strasser Lukas , Kaestle Felix , Hauck Stefanie M. , Duchrow Lukas , Aldrian Christine , Monroy Ordonez Elsa Beatriz , Luka Benedikt , Thomsen Andreas R. , Henke Michael , Gomolka Maria , Rößler Ute , Azimzadeh Omid , Moertl Simone , Hornhardt Sabine
Published: June 2023

Objective

Head and neck cancer (HNC) accounts for almost 890,000 new cases per year. Radiotherapy (RT) is used to treat the majority of these patients. A common side-effect of RT is the onset of oral mucositis, which decreases the quality of life and represents the major dose-limiting factor in RT. To understand the origin of oral mucositis, the biological mechanisms post-ionizing radiation (IR) need to be clarified. Such knowledge is valuable to develop new treatment targets for oral mucositis and markers for the early identification of “at-risk” patients.

Methods

Primary keratinocytes from healthy volunteers were biopsied, irradiated in vitro (0 and 6 Gy), and subjected to mass spectrometry-based analyses 96 h after irradiation. Web-based tools were used to predict triggered biological pathways. The results were validated in the OKF6 cell culture model. Immunoblotting and mRNA validation was performed and cytokines present in cell culture media post-IR were quantified.

Results

Mass spectrometry-based proteomics identified 5879 proteins in primary keratinocytes and 4597 proteins in OKF6 cells. Amongst them, 212 proteins in primary keratinocytes and 169 proteins in OKF6 cells were differentially abundant 96 h after 6 Gy irradiation compared to sham-irradiated controls. In silico pathway enrichment analysis predicted interferon (IFN) response and DNA strand elongation pathways as mostly affected pathways in both cell systems. Immunoblot validations showed a decrease in minichromosome maintenance (MCM) complex proteins 2-7 and an increase in IFN-associated proteins STAT1 and ISG15. In line with affected IFN signalling, mRNA levels of IFNβ and interleukin 6 (IL-6) increased significantly following irradiation and also levels of secreted IL-1β, IL-6, IP-10, and ISG15 were elevated.

Conclusion

This study has investigated biological mechanisms in keratinocytes post-in vitro ionizing radiation. A common radiation signature in keratinocytes was identified. The role of IFN response in keratinocytes along with increased levels of pro-inflammatory cytokines and proteins could hint towards a possible mechanism for oral mucositis.
Beyond the known phenotype of sotos syndrome: a 31-individuals cohort study
Authors: Lourdes Vega-Hanna , Mario Sanz-Cuesta , Didac Casas-Alba , Mercè Bolasell , Loreto Martorell , Leticia Pías , Lucia Feller Ana , Martínez-Monseny Antonio Federico , Mercedes Serrano
Published: June 2023

Introduction

Sotos Syndrome (SS, OMIM#117550) is a heterogeneous genetic condition, recognized by three main clinical features present in most cases: overgrowth with macrocephaly, typical facial appearance and different degrees of intellectual disability. Three different types are described caused by variants or deletions/duplications in NSD1, NFIX and APC2 genes. We aimed to describe a cohort of pediatric patients reporting the typical and unexpected findings in order to expand the phenotype of this syndrome and trying to find genotype-phenotype correlations.

Methods

In our referral center, we collected and analyzed clinical and genetic data of 31-patients cohort diagnosed with SS.

Results

All of them presented with overgrowth, typical dysmorphic features and different degree of developmental delay. Although structural cardiac defects have been reported in SS, non-structural diseases such as pericarditis were outstanding in our cohort. Moreover, we described here novel oncological malignancies not previously linked to SS such as splenic hamartoma, retinal melanocytoma and acute lymphocytic leukemia. Finally, five patients suffered from recurrent onychocryptosis that required surgical procedures, as an unreported prevalent medical condition.

Discussion

This is the first study focusing on multiple atypical symptoms in SS at the time that revisits the spectrum of clinical and molecular basis of this heterogeneous entity trying to unravel a genotype-phenotype correlation.
Efficacy and safety of inclisiran in stroke or cerebrovascular disease prevention: a systematic review and meta-analysis of randomized controlled trials
Authors: Luo Min , Liu Yihan , Xu Xinyi , Liu Kai , Shen Chao , Hu Haoyang , He Zhiyao , Wu Fengbo
Published: June 2023

Aims: As the impact of inclisiran in stroke prevention in atherosclerotic cardiovascular disease (ASCVD) patients or those at high risk of ASCVD is still unclear, we conducted a systematic review and meta-analysis of randomized controlled trials (RCT) to quantify the effectiveness of inclisiran in stroke prevention in these patients.

Methods: Literature research was conducted in four electronic databases (PubMed, EMBASE, Web of Science, CENTRAL) and two clinical trials registers (ClinicalTrials.gov, WHO ICTRP) from the inception of the study to 17 October 2022, and was updated by the end of the study on 5 January 2023. Two authors independently screened the studies, extracted the data, and assessed the bias. The risk of bias was assessed using the Cochrane risk-of-bias tool for randomized trials (RoB 2). The intervention effect was estimated by calculating risk ratio (RR), weighted mean difference (WMD), and 95% confidence interval (CI) with R 4.0.5. Sensitivity analysis by changing meta-analysis model was also performed to test the robustness of the pooled results. If this was not possible, a descriptive analysis was conducted.

Results: Four RCTs (n = 3,713 patients) were rated as high-risk bias. Meta-analysis of three RCTs (ORION-9, ORION-10, and ORION-11) showed that inclisiran reduced myocardial infarction (MI) risk by 32% (RR = 0.68, 95%CI = 0.48–0.96) but did not reduce stroke (RR = 0.92, 95%CI = 0.54–1.58) and major cardiovascular events (MACE) (RR = 0.81, 95%CI = 0.65–1.02) risk. Sensitivity analysis results were stable. Safety was similar to the placebo group but had frequent injection-site reactions (RR = 6.56, 95%CI = 3.83–11.25), which were predominantly mild or moderate. A descriptive analysis of one RCT (ORION-5) was conducted due to different study designs, and suggested that inclisiran might be given semiannually from the beginning.

Conclusion: Inclisiran is not beneficial for stroke or MACE prevention in ASCVD or patients at high risk of ASCVD but is associated with the reduction of MI. Given the limited number and quality of the available studies and the lack of a standardized definition for cardiovascular events, further studies are essential for confirming the results.
Genome-wide identification and functional characterization of wheat Brassinazole-resistant transcription factors in response to abiotic stresses and stripe rust infection
Authors: Zhang Peng , Yan Hanwen , Liu Yu , Chai Yi
Published: June 2023

Brassinazole-resistant (BZR) transcription factors (TFs) are key players in brassinolides (BRs) signaling pathway, which is widely involved in regulating plant growth and development, as well as in plant responding to a variety stresses. Despite their critical roles, little is known about BZR TFs in wheat. In this study, we performed genome-wide analysis of BZR gene family from wheat genome, and 20 TaBZRs were identified. Based on the phylogenetic relationships of TaBZR and BZRs from rice and Arabidopsis, all BZR genes were clustered into four groups. The intron-exon structural patterns and conserved protein motifs of TaBZRs showed high group specificity. TaBZR5, 7, and 9 were significantly induced after salt, drought treatment, and stripe rust infection. However, TaBZR16, which was significantly upregulated under NaCl application, was not expressed during wheat-stripe rust fungus interaction. These results indicated that BZR genes in wheat play different roles in response to various stresses. The results of this study will lay a foundation for further in-depth functional studies of TaBZRs and will provide information for the breeding and genetic improvement of wheat against drought and salt stresses.
Regulation of reactive oxygen species and phytohormones in osmotic stress tolerance during seed germination in indica rice
Authors: Kawaguchi Ryusuke , Suriyasak Chetphilin , Matsumoto Ryo , Sawada Yuta , Sakai Yuki , Hamaoka Norimitsu , Sasaki Kazuhiro , Yamane Koji , Kato Yoichiro , Bailly Christophe , Ishibashi Yushi
Published: June 2023

Climate change due to global warming is now affecting agricultural production worldwide. In rice, one of the most important crops, water limitation due to irregular rainfall in rainfed lowlands during crop growth limits yield. Dry direct-sowing has been proposed as a water-efficient approach to cope with water stress during rice growth, but poor seedling establishment due to drought during germination and emergence is a problem. Here, we germinated indica rice cultivars Rc348 (drought tolerant) and Rc10 (drought sensitive) under osmotic stress induced by PEG to elucidate mechanisms of germination under drought. Rc348 had higher germination rate and germination index under severe osmotic stress of −1.5 MPa, above those of Rc10. Rc348 showed up-regulated GA biosynthesis, down-regulated ABA catabolism, and up-regulated α-amylase gene expression in imbibed seeds under PEG treatment compared to that of Rc10. During germination, reactive oxygen species (ROS) play important roles in antagonism between gibberellic acid (GA) and abscisic acid (ABA). Embryo of Rc348 treated with PEG had significantly greater expression of NADPH oxidase genes and higher endogenous ROS levels, together with significantly increased endogenous GA1, GA4 and ABA contents compared to that of Rc10. In aleurone layers treated with exogenous GA, expression of α-amylase genes was higher in Rc348 than in Rc10, and expression of NADPH oxidase genes was enhanced with significantly higher ROS content in Rc348, suggesting higher sensitivity of GA to ROS production and starch degradation in aleurone cells of Rc348. These results suggest that the osmotic stress tolerance of Rc348 is due to enhancement of ROS production, GA biosynthesis, and GA sensitivity, resulting in a higher germination rate under osmotic stress.
A GBS-based genetic linkage map and quantitative trait loci (QTL) associated with resistance to Xanthomonas campestris pv. campestris race 1 identified in Brassica oleracea
Authors: Lu Lu , Choi Su Ryun , Lim Yong Pyo , Kang Si-Yong , Yi So Young
Published: June 2023

The production of Brassica oleracea, an important vegetable crop, is severely affected by black rot disease caused by the bacterial pathogen Xanthomonas campestris pv. campestris. Resistance to race 1, the most virulent and widespread race in B. oleracea, is under quantitative control; therefore, identifying the genes and genetic markers associated with resistance is crucial for developing resistant cultivars. Quantitative trait locus (QTL) analysis of resistance in the F2 population developed by crossing the resistant parent BR155 with the susceptible parent SC31 was performed. Sequence GBS approach was used to develop a genetic linkage map. The map contained 7,940 single nucleotide polymorphism markers consisting of nine linkage groups spanning 675.64 cM with an average marker distance of 0.66 cM. The F2:3 population (N = 126) was evaluated for resistance to black rot disease in summer (2020), fall (2020), and spring (2021). QTL analysis, using a genetic map and phenotyping data, identified seven QTLs with LOD values between 2.10 and 4.27. The major QTL, qCaBR1, was an area of overlap between the two QTLs identified in the 2nd and 3rd trials located at C06. Among the genes located in the major QTL interval, 96 genes had annotation results, and eight were found to respond to biotic stimuli. We compared the expression patterns of eight candidate genes in susceptible (SC31) and resistant (BR155) lines using qRT-PCR and observed their early and transient increases or suppression in response to Xanthomonas campestris pv. campestris inoculation. These results support the involvement of the eight candidate genes in black rot resistance. The findings of this study will contribute towards marker-assisted selection, additionally the functional analysis of candidate genes may elucidate the molecular mechanisms underlying black rot resistance in B. oleracea.