bluuebunny/crossref_metadata_2025 huggingface dataset:
Retinol deficiency in animals: Etiopathogenesis and consequences
Authors: Skliarov P. M. , Fedorenko S. Y. , Naumenko S. V. , Onischenko O. V. , Holda K. О.
Published: August 2020

Infertility is widespread for all species of animals and causes significant economic losses to livestock due to the loss and shortage of offspring, their reduced viability and, consequently, increased morbidity and mortality. Alimentary-deficiency factors are among the commonest causes of infertility, from which A-vitamin deficiency should be singled out. The precursor of vitamin A in the body is carotene, which is an unstable compound which is easily destroyed even under the influence of moderate factors of influence, in connection with which its deficiency is global, especially at the end of the winter – stall period of keeping animals. Accordingly it is the leading etiological factor of retinol deficiency infertility. As a result, the body has two negatives that act in parallel: carotene / vitamin A deficiency adversely affects the organs, the constituent and major functional unit of which is the secretory epithelial cell, and the free radical oxides formed in high concentration are extremely effective in destroying the cells, weakening antioxidant protection. Vitamin A has a significant effect on the reproductive function of animals both directly and indirectly. It is necessary to ensure the structure and functioning of the epithelial tissues of the organs of regulation and performance of sexual function, and therefore the physiological development of the fetus and the course of pregnancy, parturition and postpartum period, ovo- and spermiogenesis, the manifestation of sexual reflexes. Instead, its deficiency underlies the etiology and pathogenesis of retinol deficiency infertility of animals, causing changes in individual indices of homeostasis and prooxidate-antioxidant system, morphostructure of the reproductive and endocrine organs, hormonal status, sperm quality and reproductive function. The consequence is the emergence and development of gynecological, andrological, mammological and perinatal (ante-, intra-, post- and neo-) pathologies. At the same time, the addition of carotene or retinol to the diets of animals or their oral administration in cases of deficiency of vitamin A prevents impaired reproductive function. The study of the features of the etiopathogenesis of retinol deficiency infertility of animals allows programs of complex diagnostics, therapy and prevention to be developed which provide determination of carotene and vitamin A content and replenishment of the organism in cases of their deficiency.
FN1 mRNA expression of fibronectin 1 and distribution of fibronectin-associated leukocytes in humans with chronic diffuse liver diseases
Authors: Dolhikh H. V. , Maslak H. S. , Chernenko G. P. , Minchenko О. H. , Dolhikh А. О.
Published: August 2020

Chronic diffuse liver diseases are characterized by continuous progression of fibrosis, ultimately leading to cirrhosis with the following loss of the normal functioning of this organ due to excessive accumulation of the components of extracellular matrix. To find new, more available diagnostic markers of detecting disorders in the liver, we used methods of antifungal cytofluorometry and quantitative real-time polymerase chain reaction. Intensity of exposure of fibronectin and plasmatic membrane of lymphocytes in the group of patients with chronic diffuse diseases compared with the control group of practically healthy donors decreased both inside and on the surface of the cells respectively by 45.3% and 16.2%. Similar tendency towards decrease was observed during the assays of the level of the exposure of fibronectin on the surface and inside the blood granulocytes: by 25.0% and 36.5%, respectively. In the blood of the patients suffering from chronic diffuse diseases, compared with the control group, there was determined reliable increase in percentage of lymphocytes and granulocytes which contain topical fibronectin, by 32.3% and 2.78 times, correspondingly. The level of monocytes (as a percentage) with cell-associated fibronectin and fibronectin localized inside, by contrast, reliably decreased in 2.07 and 4.50 times, respectively. Analysis of the expression of FN1 in lymphocytes of blood of the studied groups using quantitative real-time polymerase chain reaction revealed decrease in the level of FN1 mRNA expression by 34.0% in the group of ill patients compared with the control group. We determined excellent diagnostic informativeness of the parameters of the level of exposure of fibronectin inside and on the surface of granulocytes and prognostic accuracy of the classifier from these parameters at the level of 100% using the method of support vector machine, SVM. High levels of diagnostic informativeness were recorded for the tests of all types of analyzed leukocytes with cell-associated fibronectin, and the classifiers based on the pair combinations of the tests with cell-associated fibronectin and fibronectin localized within the cells provide high diagnostic accuracy of the prognosis. Because the mentioned indicators are highly-sensitive tests, they can be proposed for early diagnostics and evaluation of the effectiveness of the conducted therapy of chronic diffuse liver diseases, which would allow reducing the use of paracentetic trepanobiopsy, a painful and risky procedure, which still remains the main type of diagnostic.
The lipid metabolism in carp during invasion by the tapeworn Bothriocephalus acheilognathi
Authors: Yuskiv L. L. , Yuskiv I. D.
Published: August 2020

The changes in total lipids, their fatty acid composition and the ratio of individual classes were established in tissues of the intestine, hepatopancreas and skeletal muscles of carp (Cyprinus carpio Linnaeus, 1758), with body weight 14.5–20.5 g, at different rates of invasion by Bothriocephalus acheilognathi (Yamaguti, 1934) helminth, which belongs to the family Bothriocephalidae, of the Pseudophyllidae order, of the Cestoda class. The examined carp was divided into three groups: first group of fish was free from intestinal helminths of B. acheilognathi (control); second group of fish was weakly infected with helminths (intensity of invasion is 1–3 helminths per fish); the third group of fish was highly infected (the invasion intensity is 4 worms and more per fish). Our results showed that fish infected with helminth B. acheilognathi compared to uninfected fish had reduced total lipid level in the gut due to phospholipids, triacylglycerols, and also lipids were characterized by lower content of linoleic, linolenic, arachidonic, penta- and hexanoic fatty acids; decrease in the level of unsaturated and increase in the content of saturated fatty acids, which leads to an increase of the saturation factor. During the infection of carp with B. acheilognathi in the hepatopancreas, the content of total lipids, structural lipids – phospholipids and reserve energy sources – triacylglycerols is probably reduced, and lipids are characterized by a high content of saturated fatty acids (С14:0, С16:0, С18:0) and lower content of unsaturated: arachidonic (С20:4), linolenic (С18:3) and linoleic acid (С18:2), which is associated with a decrease in the source for the synthesis of a number of polyunsaturated fatty acids, especially docosahexaenoic (С22:6). The total content of lipids, triacylglycerols, free fatty acids and phospholipids in skeletal muscle of carp during the Bothriocephalus invasion decreased and the content of free cholesterol, mono- and triacylglycerols increased. Helminth B. acheilognathi has the effect of reducing the total lipids of the skeletal muscle content of С18-, С20-, С22-polyunsaturated fatty acids and increasing the content of saturated (С14:0, С16:0, С18:0) and monounsaturated (С16:1, С18:1) fatty acids. The obtained results prove that the parasite B. acheilognathi in the intestine of the carp significantly affects the nutrition processes of the host depending on the intensity of the damage by helminths, which is accompanied by impaired lipid metabolism.
Macronutrient status and indicators of acid-alkaline blood balance in cats with chronic renal failure
Authors: Holovakha V. I. , Mostovyi E. V. , Sliusarenko A. O. , Piddubnyak O. V. , Suslova N. I. , Matsinovich M. S.
Published: August 2020

Chronic renal failure is a common pathology among cats. According to various literary sources, this pathology is found in 0.5–14.0% of cats. Pathology in cats in our studies is a consequence of glomerulonephritis. The diagnosis of chronic renal failure established on the basis of clinical, instrumental, and laboratory methods of blood and urine testing. Studies have found that chronic renal failure in cats is clinically characterized by apathy, anorexia, dry skin, odor of urea, vomiting, thirst, occurrence in the mouth of uremic ulcers (9.5% of patients), tachycardia (up to 177 beats per minute) tachypnoea (up to 45 respiratory movements per minute), hypertension. In the urine – hypostenuria, erythrocyturia, leukocyturia, kidney epithelium, hyaline and granular cylinders. According to echosonography – increase in echogenicity of the parenchyma, brain substance echone negative, cortico-brain differentiation erased. In the blood test – normochromic macrocytic anemia, thrombocytopenia, hyperazotemia (up to 27.9 mmol/L), hypercreatininemia (324 μmol/L), hyperuricemia (615 μmol/L), 4.6-fold increase in SDMA (symmetric dimethylargin). In chronic renal failure, cats have a significant change in the elemental composition of the blood. In particular, the Na+ content was increased by 3%, Ca2+ 1.6 times, P 2.1 times. Instead, the K+ level was reduced 1.9 times. Changes in acid-base equilibrium (ABE) were also detected: the pH decreased to 7.22; HCO3– by 34%, total CO2 by 32.3%, while anionic difference (AG) and buffer bases (BE) increased by 1.8 and 3.5 times, respectively. Such changes are characteristic of the development of lactic acidosis. The obtained experimental data on changes in mineral metabolism and acid-base equilibrium in the serum of cats in chronic renal failure supplement and clarify information on the pathogenic pathology of the kidneys in cats aged 5–10 years. Blood biochemical data can be used to improve early-stage diagnostics without clinical manifestations of chronic renal failure, their prevention and treatment, and to solve applied scientific problems in the field of nephrology and urology of small animals.
Low-molecular weight components of cow colostrum regulate bone marrow functions by modelling the redox-system of the organism
Authors: Bozhkov A. I. , Ohiienko S. L. , Bondar A. Y. , Ivanov E. G. , Kurguzova N. I.
Published: August 2020

Colostrum is rich in various biologically active compounds such as immunotropic ones. Low molecular weight components were isolated from cow colostrum components (with a molecular weight of not more than 45 kDa). Their influence was investigated on intact Wistar Rattus norvegicus adult males in concentrations of 0.01, 0.1, 1.0 and 5.0 g/100 g of body weight. We determined content of lipid hydroperoxides and activity of serum glutathione peroxidase in blood serum, parameters of the bone marrow cells’ (BMCs) behaviour in the in vitro system (proliferation ability, morphologically identifiable and unidentifiable type of cells, lifespan of eosinophils). Morphological identifiable cells were stab neutrophils, segmented neutrophils, metamyelocytes, myelocytes, lymphocytes, basophils, neutrophils, eosinophils, monocytes. The low doses of colostrum components (0.01–0.10 g/100 g of body weight) did not affect the ratio of morphologically identifiable/unidentifiable cells. Administration of colostrum components at low doses (0.01 g/100 g of weight) increased the ability of BMCs to proliferate in the in vitro system. A super-large dose of colostrum components (5 g/100 g of body weight) was accompanied by a further loss of capacity for proliferation and cell death. Moreover, large doses of colostrum components resulted in change of balance to prooxidants (oxidants). The role of redox – system in BMCs functions was discussed. Large doses of colostrum components (1–5 g/100 g of body weight) were accompanied by a change of pro-antioxidant system balance. Only eosinophils were determined after administration of colostrum components in a large dose. It should be noted that the lifetime of eosinophils which developed under influence of colostrum components was greater than that of eosinophils obtained from control animals.
Antibacterial and fungicidal activities of ethanol extracts from Cotinus coggygria, Rhus typhina, R. trilobata, Toxicodendron orientale, Hedera helix, Aralia elata, Leptopus chinensis and Mahonia aquifolium
Authors: Zazharskyi V. V. , Davydenko P. О. , Kulishenko O. М. , Borovik I. V. , Brygadyrenko V. V.
Published: August 2020

The search for promising plants with bactericidal and fungicidal activity is of great interest for practical and veterinary medicine, This article reveals the high antibacterial effect of the use of ethanol extracts from 8 species of plants of the families Anacardiaceae (Cotinus coggygria Scop., Rhus typhina L., Rhus trilobata Nutt. and Toxicodendron orientale Greene), Araliaceae (Hedera helix Linnaeus and Aralia elata (Miq.) Seem.), Phyllanthaceae (Leptopus chinensis (Bunge) Pojark.), Berberidaceae (Mahonia aquifolium (Pursh) Nutt.) against 23 strains of bacteria and one strain of fungi. The in vitro experiment revealed the zone of inhibition of growth of colonies exceeding 8 mm during the application of ethanol extracts of C. coggygria against twelve species of microorganisms (Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, S. epidermidis, Bacillus cereus, Listeria ivanovi, Corynebacterium xerosis, Rhodococcus equi, Proteus vulgaris, P. mirabilis, Serratia marcescens and Candida albicans), Rhus typhina – against twelve species (E. faecalis, E. coli, S. aureus, S. epidermidis, L. ivanovi, C. xerosis, Rh. equi, P. vulgaris, Salmonella typhimurium, S. adobraco, S. marcescens and C. albicans), Rhus trilobata – against fourteen (E. faecalis, E. сoli, S. аureus, S. epidermidis, B. subtilis, B. cereus, L. ivanovi, C. xerosis, Rh. equi, P. vulgaris, P. mirabilis, Рseudomonas аeruginosa, Yersinia enterocolitica and C. albicans), Toxicodendron orientale – against eleven (E. faecalis, S. аureus, L. іnnocua, C. xerosis, Campylobacter jejuni, Rh. equi, P. vulgaris, P. mirabilis, Р. аeruginosa and C. albicans), Hedera helix – against seven (S. аureus, S. epidermidis, L. monocytogenes, C. jejuni, Rh. equi, P. vulgaris and C. albicans), Aralia elata – against nine (E. coli, S. aureus, B. cereus, C. xerosis, P. vulgaris, P. mirabilis, S. typhimurium, S. marcescens and C. albicans), Leptopus chinensis – only against four (E. coli, S. epidermidis, B. cereus and P. mirabilis) and Mahonia aquifolium – against only three species (S. epidermidis, C. jejuni and P. vulgaris). As a result of the research, the most promising for studying in future regarding in vivo antibacterial activity were determined to be C. coggygria, Rhus typhina, R. trilobata, Toxicodendron orientale and Aralia elata.
Modification of isolation methods and physico-biochemical properties of preparations of fungal oxidoreductases
Authors: Fedotov O. V. , Usikova Z. L.
Published: August 2020

The results of the modification of methods for producing enzymatic preparations (EP) of peroxidases and catalases of extra- and intracellular finding from fungal cultures are presented. Strains of Flammulina velutipes F-vv, Lentinula edodes 523 and Pleurotus ostreatus P-01 were used as producers of oxidoreductases. The producers were cultured in glucose-peptone medium, modified for each strain. Protein fractionation was carried out by leaching with ammonium sulfate at a saturation of 40–70% for peroxidases and 80% for catalases. The obtained solutions of protein fractions were additionally subjected to purification by dialysis, gel filtration on Molselect G-50 and G-75 granules, and also freeze drying. The yield of enzymatic preparations per unit mass of mycelium and the volume of culture fluid were calculated. The individual characteristics of EP – enzymatic activity, the mass percentage of protein and associated amino acids, the ratio of the latter in groups depending on the nature of the radicals (amphotericity) of protein molecules are established. It was proved that the amino acid content in the proteins of fungal EP catalases and peroxidases indicates their acidic nature and this is confirmed by the pH values of aqueous solutions. Examination and toxicity testing of enzymatic preparations were carried out in certified laboratories, which confirmed their characteristics and compliance with safety requirements. The therapeutic properties of amino acids that are part of proteins or are in a free state in enzyme preparations are analyzed. In this way, the methods have been developed for producing enzymatic preparations of peroxidases and catalases of extra- and intracellular location, which allow new antioxidant enzymes with individual properties to be obtained, and, as a result, bring prospects for use in various industries and scientific research.
Morphological and radiological study of lymph nodes in dromedaries in Algeria
Authors: Rahmoun D. E. , Lieshchova M. A. , Fares M. A.
Published: August 2020

Despite significant progress in the study of the subtle mechanisms of interaction between cellular and molecular elements in immune responses, the general structure of the organs of the immune system, including the lymph node, has not been sufficiently studied, in particular in large farm animals. The lymph nodes of sexually mature camels have been studied anatomically and morphologically and advanced studies conducted using an X-ray system and a computer densitometer scanner with injection of a contrast medium. The topography and characteristics of the morphometric parameters (absolute and relative mass, linear measurements, volume) of certain somatic and visceral lymph nodes were determined. The mass of the lymph nodes studied varies according to the location and the interest of the organ in the satellite defense of the lymphoid system, For part of the x-ray examination of the lymph nodes, organs of large inguinal and axillary shape were selected after passing through a solution of tetraethyl-4,4-diamino-triphenylmethane oxalate, the lymph vessels were dilated and darkened, then iodine injections were made into the afferent lymphatic vessel of two lymph nodes; they were placed on the radiological cassette, a photograph taken on conventional radiography, for computer densitometer, the examination was made without preparation of the organs. A capsule encompasses the parenchyma of the lymph node, whose internal structure is composed of different zones, cortical, paracortical and medullary, on the one hand the lymphatic vessels were very clear especially with the conventional radiography with preparation of the organs, while the computer densitometer clearly revealed the deep texture of the parenchyma, basing it on the intensity of emission saturation from the use of computer densitometer.
Evde bakım alanlarda düşme riskinin değerlendirilmesi
Authors: Çubukçu Mahcube
Published: August 2020

Amaç: Bu çalışmada evde bakıma kayıtlı hastaların düşme ile ilişkili risk faktörlerini belirlemek, düşme sıklığını saptamak ve bu sonuçlar doğrultusunda toplum düzeyinde düşmenin önlenmesi stratejilerinin etkinliğini artırmak amaçlanmıştır.Yöntem: Bu tanımlayıcı çalışma 01.01.2015-28.02.2015 tarihleri arasında yapıldı. 184 hasta Samsun Eğitim ve Araştırma Hastanesi Evde Bakım Birimince evlerinde ziyaret edildi. Aydınlatılmış onamı alınan hasta veya hasta yakınlarına sosyodemografik bilgilerini içeren anket formu ve İtaki Düşme Riski Ölçeği yüz yüze uygulandı. Veriler SPSS 20.00 programı ile değerlendirildi. Verilerin değerlendirilmesinde Pearson ki kare ve Mann-Whitney U testi uygulandı. p
Poliklinik başvurularında obezite ve depresyon ilişkisinin incelenmesi
Authors: Çakmur Hülya , Güneş Ümit Baran
Published: August 2020

Amaç: Obezite ve depresyon, tüm dünyada ve ülkemizde, sıklığı giderek artan önemli bir sağlık sorunudur. Bu çalışmanın amacı; obez ve obez olmayan katılımcılarda obezite ve depresyon arasında ilişki olup olmadığını incelemektir.Yöntem: Bu çalışmaya 18 - 65 yaş grubunda, 92 obez ve 107 obez olmayan toplam 199 birey alınmıştır. Yaş grupları, ortalamaya göre sınıflandırılarak incelenmiştir. Beden bileşimi ölçümleri, Biyoelektriksel İmpedans analizi ile yapılmıştır. Obezite, Dünya Sağlık Örgütü tanımlanmasına göre; Beden Kütle İndeksi ≥30 olarak sınıflandırılmıştır. Hamilton Depresyon Derecelendirme Ölçeği kullanılarak her iki grupta depresyon durumu incelenmiştir. Depresyon, tüm katılımcıların ölçek puan ortalamasının yer aldığı gruba göre derecelendirilmiştir.Bulgular: Her iki grupta toplam 118 olguda “hafif depresyon” saptanmıştır (%59,3). Obez olgularda kadınlarda depresyon riski 3,1 kat (OR=3.111, GA:1.262-7.667) yüksek ve fark istatistiksel olarak anlamlı bulunmuştur. Obez olmayan olgularda depresyon riski kadınlarda 2,4 kat (OR=2.449, %95 GA=0.939-4.387) yüksek bulunmuş, ancak güven aralığının “1” değerini içerdiği ve farkın istatistiksel anlamlılık değerine yakın olduğu görülmüştür. Tüm katılımcılarda, depresyon riski kadınlarda 2,7 kat (OR=2.680, GA: 1.246-5.938) yüksek bulunmuş ve farkın istatistiksel olarak anlamlı olduğu görülmüştür. Obez olgularda depresyon riskinin obez olmayan olgulara göre 2,9 kat (OR=2.453, GA:1.363-6.713) yüksek olduğu ve farkın istatistiksel olarak anlamlı olduğu saptanmıştır.Sonuç: Bu çalışmada, obez ve kadın katılımcılarda depresyon riski, obez olmayanlara ve erkeklere göre istatistiksel olarak anlamlı ölçüde yüksek bulunmuştur.