bluuebunny/crossref_metadata_2025 huggingface dataset:
Mannose-binding lectin gene polymorphism in psoriasis and vitiligo: an observational study and computational analysis
Authors: Behairy Mohammed Y. , Tawfik Noha Z. , Eid Refaat A. , Nasser Binjawhar Dalal , Alshaya Dalal Sulaiman , Fayad Eman , Elkhatib Walid F. , Abdallah Hoda Y.
Published: February 2024

Introduction

Psoriasis and vitiligo are inflammatory autoimmune skin disorders with remarkable genetic involvement. Mannose-binding lectin (MBL) represents a significant immune molecule with one of its gene variants strongly linked to autoimmune diseases. Therefore, in this study, we investigated the role of the MBL variant, rs1800450, in psoriasis and vitiligo disease susceptibility.

Methods

The study comprised performing in silico analysis, performing an observational study regarding psoriasis patients, and performing an observational study regarding vitiligo patients. Various in silico tools were used to investigate the impact of the selected mutation on the function, stability, post-translational modifications (PTMs), and secondary structures of the protein. In addition, a total of 489 subjects were enrolled in this study, including their demographic and clinicopathological data. Genotyping analysis was performed using real-time PCR for the single nucleotide polymorphism (SNP) rs1800450 on codon 54 of the MBL gene, utilizing TaqMan genotyping technology. In addition, implications of the studied variant on disease susceptibility and various clinicopathological data were analyzed.

Results

Computational analysis demonstrated the anticipated effects of the mutation on MBL protein. Furthermore, regarding the observational studies, rs1800450 SNP on codon 54 displayed comparable results in our population relative to global frequencies reported via the 1,000 Genomes Project. This SNP showed no significant association with either psoriasis or vitiligo disease risk in all genetic association models. Furthermore, rs1800450 SNP did not significantly correlate with any of the demographic or clinicopathological features of both psoriasis and vitiligo.

Discussion

Our findings highlighted that the rs1800450 SNP on the MBL2 gene has no role in the disease susceptibility to autoimmune skin diseases, such as psoriasis and vitiligo, among Egyptian patients. In addition, our analysis advocated the notion of the redundancy of MBL and revealed the lack of significant impact on both psoriasis and vitiligo disorders.
Fluid mechanics of aortic valve incompetence in the dilated left ventricle
Authors: Vu Vi , May-Newman Karen
Published: February 2024

Introduction: Aortic insufficiency (AI) occurs when the aortic valve fails to close completely, allowing backward blood flow into the left ventricle (LV). The progression of AI can lead to ventricular dysfunction and congestive heart failure, setting off a self-perpetuating cycle that worsens these conditions. This study employed models of repeatable and reversible AI within a simulated circulatory loop to analyze vortex dynamics, AI parameters, and gain insights into the efficiency of ventricular washout.

Method: A transparent silicone model of an LV with an ejection fraction of 17% served as the baseline, simulating a condition without AI. Mild, moderate, and severe AI were induced using 3D-printed stents, obstructing the complete closure of the aortic valve while allowing unimpeded forward blood flow. Midplane velocity fields were analyzed to compute AI and vortex properties, energy dissipation rate, blood residence time, and shear activation potential.

Results and discussion: With increasing AI severity, the regurgitant jet expanded, impeding the development and trajectory of mitral inflow. The inefficiency in fluid transport became apparent through a declining ratio of total kinetic energy rate to energy dissipation rate and an increasing residence time. Impaired ventricular washout resulted in the accumulation of fluid with elevated shear activation potential in the LV. These findings suggested that AI progressively induces abnormal intraventricular flow, heightening the thromboembolic risk in heart failure patients. The study also advocates for the potential application of mock circulatory system to explore the effects of various AI configurations, especially when combined with other cardiac implants like artificial heart valve or left ventricular assist device.
Analytical validation and clinical utilization of K-4CARE™: a comprehensive genomic profiling assay with personalized MRD detection
Authors: Nguyen Hoang Thien-Phuc , Nguyen Tien Anh , Tran Nam H. B. , Nguyen Hoang Van-Anh , Thi Dao Hong Thuy , Tran Vu-Uyen , Nguyen Yen Nhi , Nguyen Anh Tuan , Nguyen Thi Cam Tu , Do Thi Thanh Thuy , Nguyen Duy Sinh , Nguyen Hoai-Nghia , Giang Hoa , Tu Lan N.
Published: February 2024

Background: Biomarker testing has gradually become standard of care in precision oncology to help physicians select optimal treatment for patients. Compared to single-gene or small gene panel testing, comprehensive genomic profiling (CGP) has emerged as a more time- and tissue-efficient method. This study demonstrated in-depth analytical validation of K-4CARE, a CGP assay that integrates circulating tumor DNA (ctDNA) tracking for residual cancer surveillance.

Methods: The assay utilized a panel of 473 cancer-relevant genes with a total length of 1.7 Mb. Reference standards were used to evaluate limit of detection (LOD), concordance, sensitivity, specificity and precision of the assay to detect single nucleotide variants (SNVs), small insertion/deletions (Indels), gene amplification and fusion, microsatellite instability (MSI) and tumor mutational burden (TMB). The assay was then benchmarked against orthogonal methods using 155 clinical samples from 10 cancer types. In selected cancers, top tumor-derived somatic mutations, as ranked by our proprietary algorithm, were used to detect ctDNA in the plasma.

Results: For detection of somatic SNVs and Indels, gene fusion and amplification, the assay had sensitivity of >99%, 94% and >99% respectively, and specificity of >99%. Detection of germline variants also achieved sensitivity and specificity of >99%. For TMB measurement, the correlation coefficient between whole-exome sequencing and our targeted panel was 97%. MSI analysis when benchmarked against polymerase chain reaction method showed sensitivity of 94% and specificity of >99%. The concordance between our assay and the TruSight Oncology 500 assay for detection of somatic variants, TMB and MSI measurement was 100%, 89%, and 98% respectively. When CGP-informed mutations were used to personalize ctDNA tracking, the detection rate of ctDNA in liquid biopsy was 79%, and clinical utility in cancer surveillance was demonstrated in 2 case studies.

Conclusion: K-4CARE™ assay provides comprehensive and reliable genomic information that fulfills all guideline-based biomarker testing for both targeted therapy and immunotherapy. Integration of ctDNA tracking helps clinicians to further monitor treatment response and ultimately provide well-rounded care to cancer patients.
Evaluating speech-in-speech perception via a humanoid robot
Authors: Meyer Luke , Araiza-Illan Gloria , Rachman Laura , Gaudrain Etienne , Başkent Deniz
Published: February 2024

Introduction

Underlying mechanisms of speech perception masked by background speakers, a common daily listening condition, are often investigated using various and lengthy psychophysical tests. The presence of a social agent, such as an interactive humanoid NAO robot, may help maintain engagement and attention. However, such robots potentially have limited sound quality or processing speed.

Methods

As a first step toward the use of NAO in psychophysical testing of speech- in-speech perception, we compared normal-hearing young adults’ performance when using the standard computer interface to that when using a NAO robot to introduce the test and present all corresponding stimuli. Target sentences were presented with colour and number keywords in the presence of competing masker speech at varying target-to-masker ratios. Sentences were produced by the same speaker, but voice differences between the target and masker were introduced using speech synthesis methods. To assess test performance, speech intelligibility and data collection duration were compared between the computer and NAO setups. Human-robot interaction was assessed using the Negative Attitude Toward Robot Scale (NARS) and quantification of behavioural cues (backchannels).

Results

Speech intelligibility results showed functional similarity between the computer and NAO setups. Data collection durations were longer when using NAO. NARS results showed participants had a relatively positive attitude toward “situations of interactions” with robots prior to the experiment, but otherwise showed neutral attitudes toward the “social influence” of and “emotions in interaction” with robots. The presence of more positive backchannels when using NAO suggest higher engagement with the robot in comparison to the computer.

Discussion

Overall, the study presents the potential of the NAO for presenting speech materials and collecting psychophysical measurements for speech-in-speech perception.
Gut microbiota, nutrition, and mental health
Authors: Merlo Gia , Bachtel Gabrielle , Sugden Steven G.
Published: February 2024

The human brain remains one of the greatest challenges for modern medicine, yet it is one of the most integral and sometimes overlooked aspects of medicine. The human brain consists of roughly 100 billion neurons, 100 trillion neuronal connections and consumes about 20–25% of the body’s energy. Emerging evidence highlights that insufficient or inadequate nutrition is linked to an increased risk of brain health, mental health, and psychological functioning compromise. A core component of this relationship includes the intricate dynamics of the brain-gut-microbiota (BGM) system, which is a progressively recognized factor in the sphere of mental/brain health. The bidirectional relationship between the brain, gut, and gut microbiota along the BGM system not only affects nutrient absorption and utilization, but also it exerts substantial influence on cognitive processes, mood regulation, neuroplasticity, and other indices of mental/brain health. Neuroplasticity is the brain’s capacity for adaptation and neural regeneration in response to stimuli. Understanding neuroplasticity and considering interventions that enhance the remarkable ability of the brain to change through experience constitutes a burgeoning area of research that has substantial potential for improving well-being, resilience, and overall brain health through optimal nutrition and lifestyle interventions. The nexus of lifestyle interventions and both academic and clinical perspectives of nutritional neuroscience emerges as a potent tool to enhance patient outcomes, proactively mitigate mental/brain health challenges, and improve the management and treatment of existing mental/brain health conditions by championing health-promoting dietary patterns, rectifying nutritional deficiencies, and seamlessly integrating nutrition-centered strategies into clinical care.
A study on out-of-field leakage of an accelerator-based neutron beam for boron neutron capture therapy
Authors: You Zhen-Fan , Huang Chun-Kai , Liu Yen-Wan Hsueh
Published: February 2024

More and more accelerator-based boron neutron capture therapy (AB-BNCT) facilities are under the construction or commissioning stage, and the neutron beam characteristic measurements at each facility will start soon. In addition to the in-field neutron beam properties, the leakage of neutron beam is also important, which is related to the side effects of the patient. In the Virtual Technical Meeting on Advances in Boron Neutron Capture Therapy held by International Atomic Energy Agency (IAEA) in July 2020, the issue of the out-of-field leakage in BNCT was addressed. Heron Neutron Medical Corporation has been working on the beam design for China Medical University Hsinchu Hospital AB-BNCT research center. To evaluate the out-of-field leakage, both beam profile analysis and whole-body dose calculation are performed. An Oak Ridge National Laboratory (ORNL) Medical Internal Radiation Dose (MIRD) mathematical phantom is used to calculate the whole-body dose. For the estimated irradiation time which is set to be the time required for 80% of tumor dose to reach 20 Gy-w, the relative biological effectiveness weighted dose of abdomen region is less than 40 mGy-w and the whole-body dose is 104 mSv. The beam profile calculational result shows that the neutron ambient dose equivalent at 15 cm from the field edge is 11 mSv/Gy-w and drops to 5 mSv/Gy-w at 26 cm from the field edge. The gamma ray ambient dose equivalent is less than 1 mSv/Gy-w starting from 10 cm from the field edge. Although the neutron out-of-field leakage of the beam design is higher than that of the initially proposed guideline by IAEA in 2020, the whole-body dose, however, is reasonably low. Both the whole-body dose evaluation and the beam profile analysis are useful in the beam design consideration. The whole-body dose calculation together with the beam profile analysis can also be helpful in reaching an acceptable recommendation for the out-of-field leakage for BNCT neutron beam, a job wished to be accomplished in the near future as proposed in the 2023 IAEA’s report on Advances in Boron Neutron Capture Therapy.
Analysis of screening outcomes and factors influencing compliance among community-based lung cancer high-risk population in Nanchang, China, 2018-2020
Authors: Zeng Fanfan , Wang Xiaobo , Wang Chengman , Zhang Yu , Fu Denggang , Wang Xin
Published: February 2024

Objective

To investigate the screening results and compliance of low-dose computed tomography (LDCT) screening among the high-risk lung cancer populations in Jiangxi Province from 2018 to 2020, and to explore the related influencing factors of compliance.

Methods

From November 2018 to October 2020, permanent residents in Nanchang City were selected and their demographic data and lung cancer risk factor data were collected to screen high-risk groups, and LDCT screening was performed on high-risk groups with diagnostic reports by 2 chief physicians. Descriptive analysis method was used to analyze the basic information of screening, screening results and screening compliance. χ2 and logistic regression test were used to conduct single and multi-factor analysis of screening compliance.

Results

A total of 26,588 people participated in this screening, of which 34.4% (n=9,139) were at high risk of lung cancer, 3,773 participants were completed LDCT screening, and the screening compliance rate was 41.3%. Screening results showed that 389 participants were positive for suspected pulmonary tumor or lung nodules, the screening positive rate of 10.3%. The logistic multivariable results of screening compliance showed that the compliance was better in males, those who quit smoking, those with chronic respiratory diseases and family history of cancer, and those who have primary education, those with a history of occupational harmful exposure had a poor compliance.

Conclusion

Compliance with lung cancer screening in Jiangxi Province, China still needs to be improved, and gender, education level, harmful occupational exposure, smoking, chronic respiratory diseases, and family history of tumors cancer play an important role on screening compliance.
Adeno-associated virus-mediated gene transfer of arginine decarboxylase to the central nervous system prevents opioid analgesic tolerance
Authors: Churchill Caroline C. , Peterson Cristina D. , Kitto Kelley F. , Pflepsen Kelsey R. , Belur Lalitha R. , McIvor R. Scott , Vulchanova Lucy , Wilcox George L. , Fairbanks Carolyn A.
Published: February 2024

Agmatine, a decarboxylated form of L-arginine, prevents opioid analgesic tolerance, dependence, and self-administration when given by both central and systemic routes of administration. Endogenous agmatine has been previously detected in the central nervous system. The presence of a biochemical pathway for agmatine synthesis offers the opportunity for site-specific overexpression of the presumptive synthetic enzyme for local therapeutic effects. In the present study, we evaluated the development of opioid analgesic tolerance in ICR-CD1 mice pre-treated with either vehicle control or intrathecally delivered adeno-associated viral vectors (AAV) carrying the gene for human arginine decarboxylase (hADC). Vehicle-treated or AAV-hADC-treated mice were each further divided into two groups which received repeated delivery over three days of either saline or systemically-delivered morphine intended to induce opioid analgesic tolerance. Morphine analgesic dose-response curves were constructed in all subjects on day four using the warm water tail flick assay as the dependent measure. We observed that pre-treatment with AAV-hADC prevented the development of analgesic tolerance to morphine. Peripheral and central nervous system tissues were collected and analyzed for presence of hADC mRNA. In a similar experiment, AAV-hADC pre-treatment prevented the development of analgesic tolerance to a high dose of the opioid neuropeptide endomorphin-2. Intrathecal delivery of anti-agmatine IgG (but not normal IgG) reversed the inhibition of endomorphin-2 analgesic tolerance in AAV-hADC-treated mice. To summarize, we report here the effects of AAV-mediated gene transfer of human ADC (hADC) in models of opioid-induced analgesic tolerance. This study suggests that gene therapy may contribute to reducing opioid analgesic tolerance.
Acellular dermal matrix in urethral reconstruction
Authors: Aodi Jiang , Ying Lian , Chengyang Sun , Hongfeng Zhai
Published: February 2024

The management of severe urethral stricture has always posed a formidable challenge. Traditional approaches such as skin flaps, mucosal grafts, and urethroplasty may not be suitable for lengthy and intricate strictures. In the past two decades, tissue engineering solutions utilizing acellular dermal matrix have emerged as potential alternatives. Acellular dermal matrix (ADM) is a non-immunogenic biological collagen scaffold that has demonstrated its ability to induce layer-by-layer tissue regeneration. The application of ADM in urethral reconstruction through tissue engineering has become a practical endeavor. This article provides an overview of the preparation, characteristics, advantages, and disadvantages of ADM along with its utilization in urethral reconstruction via tissue engineering.
Wavelength dependent transmission in multimode graded-index microstructured polymer optical fibers
Authors: Simović Ana , Savović Svetislav , Wang Zhuo , Drljača Branko , Kovačević Milan S. , Kuzmanović Ljubica , Djordjevich Alexandar , Aidinis Konstantinos , Chen Chen
Published: February 2024

Up to now, there have been no commercial simulation tools accessible for researching the transmission properties of multimode microstructured optical fibers (MOFs). In order to avoid this problem, this study uses the time-independent power flow equation (TI PFE) numerical solution to examine the wavelength dependency of the equilibrium mode distribution (EMD) and steady state distribution (SSD) in multimode graded-index microstructured polymer optical fibers (GI mPOF) with a solid core. We showed that the lengths zs at which an SSD is obtained in GI mPOF and the coupling length Lc necessary to create an EMD are shorter at λ = 568 nm than they are found to be at λ = 633 nm. The lengths Lc and zs stay constant when the wavelength decreases further from λ = 568 to 522 and then to 476 nm. As a result, it is anticipated that a faster bandwidth enhancement in the tested GI mPOF will take place at wavelengths around λ = 568 nm as opposed to λ = 633 nm. Such a bandwidth improvement is not brought about by additional wavelength reduction. The study’s findings can be used in communication and sensory systems that use multimode GI mPOFs at different wavelengths.
Walking around the preferred speed: examination of metabolic, perceptual, spatiotemporal and stability parameters
Authors: Majed Lina , Ibrahim Rony , Lock Merilyn Jean , Jabbour Georges
Published: February 2024

Walking is the most accessible and common type of physical activity. Exercising at one’s self-selected intensity could provide long-term benefits as compared to following prescribed intensities. The aim of this study was to simultaneously examine metabolic, perceptual, spatiotemporal and stability parameters at an absolute 3 km·h−1 speed range around the individual preferred walking speed (PWS). Thirty-four young sedentary adults (18 women) volunteered to walk at seven speeds relative to their PWS in 3-min trials interspaced with 3-min rest intervals. Results indicated a significant main effect of speed on all studied variables. While metabolic, perceptual and spatiotemporal values were sensitive to the smallest change in speed (i.e., 0.5 km·h−1), a significant increase in the rate of carbohydrate oxidation and decrease in %fat oxidation were only observed at speeds above PWS. Results also revealed significantly higher coefficients of variation for stride characteristics at speeds below PWS only. Moreover, analyses of best fit models showed a quadratic relationship between most variables and speed, with the exceptions of metabolic cost of transport, rating of perceived exertion and stride duration that changed exponentially with speed. PWS coincided with optimized mechanical efficiency, fuel oxidation and gait stability. This indicated that walking below PWS decreased both mechanical efficiency and stability of gait, while walking above PWS increased carbohydrate oxidation. Those factors seem to play an important role as determinants of PWS. We suggest that walking at PWS may provide benefits in terms of fat oxidation while optimizing gait stability.
Improving U-net network for semantic segmentation of corns and weeds during corn seedling stage in field
Authors: Cui Jiapeng , Tan Feng , Bai Nan , Fu Yaping
Published: February 2024

Introduction

Weeds are one of the main factors affecting crop growth, making weed control a pressing global problem. In recent years, interest in intelligent mechanical weed-control equipment has been growing.

Methods

We propose a semantic segmentation network, RDS_Unet, based on corn seedling fields built upon an improved U-net network. This network accurately recognizes weeds even under complex environmental conditions, facilitating the use of mechanical weeding equipment for reducing weed density. Our research utilized field-grown maize seedlings and accompanying weeds in expansive fields. We integrated the U-net semantic segmentation network, employing ResNeXt-50 for feature extraction in the encoder stage. In the decoder phase, Layer 1 uses deformable convolution with adaptive offsets, replacing traditional convolution. Furthermore, concurrent spatial and channel squeeze and excitation is incorporated after ordinary convolutional layers in Layers 2, 3, and 4.

Results

Compared with existing classical semantic segmentation models such as U-net, Pspnet, and DeeplabV3, our model demonstrated superior performance on our specially constructed seedling grass semantic segmentation dataset, CGSSD, during the maize seedling stage. The Q6mean intersection over union (MIoU), precision, and recall of this network are 82.36%, 91.36%, and 89.45%, respectively. Compared to those of the original network, the proposed network achieves improvements of 5.91, 3.50, and 5.49 percentage points in the MIoU, precision, and recall, respectively. The detection speed is 12.6 frames per second. In addition, ablation experiments further confirmed the impactful contribution of each improvement component on the overall semantic segmentation performance.

Discussion

This study provides theoretical and technical support for the automated operation of intelligent mechanical weeding devices.
Nonverbal cues to deception: insights from a mock crime scenario in a Chinese sample
Authors: Li He , Song Hu , Li Menghan , Li Hanxue
Published: February 2024

Nonverbal behaviors could play a crucial role in detecting deception, yet existing studies on deception cues have largely centered on Western populations, predominantly university students, thus neglecting the influence of cultural and sample diversity. To address this gap, our study explored deception cues within an Asian cultural setting, utilizing a mock crime paradigm. Our sample comprised Chinese participants, including both men and women with various socioeconomic status (SES) backgrounds. Our findings revealed that compared to truth tellers, liars exhibited heightened emotions and an increased cognitive load. Furthermore, liars showed a higher frequency of self-adaptors and a longer duration of gaze aversion. Our findings contribute to a more profound understanding of deception cues within Asian culture and have implications for practical fields such as criminal interrogation.
The interdependence of substance use, satisfaction with life, and psychological distress: a dynamic structural equation model analysis
Authors: Moe Fredrik D. , Erga Aleksander , Bjornestad Jone , Dettweiler Ulrich
Published: February 2024

Introduction

Longitudinal studies with annual follow-up including psychological and social variables in substance use disorder recovery are scarce. We investigated whether levels of substance use, satisfaction with life, and psychological distress fluctuate across five years in relation to having drug-free friends.

Methods

A prospective naturalistic cohort study of change trajectories in a cohort of people diagnosed with substance use disorder and using multiple substances with quarterly and annual follow-up over five years. Two-hundred-and-eight patients were recruited from substance use disorder treatment in Rogaland, Norway. Out of these, 164 participants fulfilled the inclusion criteria. We used Bayesian two-level dynamic structural equation modelling. The variable ‘drug-free friends’ was assessed by a self-reporting questionnaire, while psychological distress was assessed using the Symptoms Checklist 90 Revised. Satisfaction with life was assessed using the Satisfaction With Life Scale while drug use was assessed using the Drug Use Disorders Identification Test.

Results

The main findings are that higher-than-average psychological distress at a three-month lag credibly predicts higher-than-normal substance use at the concurrent time point t. Substance use and satisfaction with life seem to have synchronous trajectories over time, i.e. as the first decreases the latter increases and vice versa. During the five years after treatment, the participants mainly experienced a decrease in substance use and increase in satisfaction with life.

Conclusion

Since the participants experienced positive and negative fluctuations for several years after treatment, it seems crucial to establish a dialogue with treatment professionals in order to create functional solutions for maintaining motivation and aiding recovery.
Curricula and resources related to social entrepreneurship and public health innovation within schools of public health in the United States
Authors: Hyde Ingeborg , Khoshnood Kaveh , Chahine Teresa , Basrai Fatema
Published: February 2024

This paper examines the current state of social innovation and entrepreneurship programming, courses, and centers within schools of public health through a survey data analysis. This report presents a cross-sectional survey conducted among faculty members of public health schools in the United States. The survey aims to determine the availability and current state of student-centered programs and courses centered around social innovation and entrepreneurship within schools of public health. Insights were drawn from 19 professionals across 15 schools of public health. Uncertainties surround the sustainability of current programs, with insufficient funding, human resources, and the need to teach more pressing topics identified as the most significant obstacles. Key areas identified as opportunities for growth were faculty engagement, expertise, and funding to expand more structured programming.
Foreign language learning using augmented reality environments: a systematic review
Authors: Schorr Isabel , Plecher David A. , Eichhorn Christian , Klinker Gudrun
Published: February 2024

Augmented Reality (AR) is an advancing technology that has drawn the attention of educational material designers across various academic fields. However, few studies document the successes and setbacks of AR research in the language education sector. This review delves into educational research that employs AR for language training, examining the existing literature on this topic for development trends, benefits, challenges, and success patterns to derive design principles from them. In doing so, the paper covers 40 studies published between 2016 and 2023. The findings suggest that AR is mainly used for vocabulary acquisition with a clear trend toward applying marker-based technology and mobile devices. The design principles derived indicate that the potential of AR lies primarily in contextual learning, and that the technology alone may not satisfy students’ needs in all aspects of language learning but should be used in combination with traditional teaching methods.
Recovery markers in elite climbers after the national boulder climbing championship
Authors: Gáspari Arthur Fernandes , Zaminiani Mayni Gabriele , Vilarinho Manoela de Carvalho , Caruso Danilo , Guimarães Patricia dos Santos , Piunti Rafael Perlotti , Itaborahy Alex , de Moraes Antonio Carlos
Published: February 2024

This study aimed to investigate recovery markers among elite climbers following the National Boulder Championship. We assessed maximum isometric hand grip strength (HS), forearm swelling (circumference), delayed soreness in forearm muscles, tiredness, and exercise readiness at several time points: pre-competition, immediately post-competition (within 4 min after their last effort), and 12, 24, 48, and 60 h post-competition. Maximum isometric hand grip strength decreased by 6.38 ± 1.32% (p = 0.006) post-12 h, returning to pre-competition values post-24 h (allp > 0.05). Forearm circumference (FC) increased 1.78 ± 1.77% (p < 0.001) post-competition, returning to pre-competition values post-12 h (allp > 0.05). Forearm pain (FP) increased post-competition (p = 0.002) and post-12 h (p < 0.001), returning to pre-competition values post-24 h (allp > 0.05). Tiredness increased post-competition (p < 0.001), post-12 h (p < 0.001), and post-24 h (p < 0.001), returning to pre-competition values post-48 h (allp > 0.05). Climbing readiness was reduced post-competition (p < 0.001), post-12 h (p < 0.001), post-24 h (p < 0.001), and post-48 h (p = 0.005), only returning to pre-competition values post-60 h (p = 0.189). Visual analysis of individual data pointed out a relatively small variability in the HS and FC markers, while FP, tiredness, and readiness exhibited larger individual variations. These findings indicate that different recovery patterns exist for the analyzed markers, suggesting that athletes may require up to 60 h after a competition to fully recover and regain their ability to face new competitive challenges.
Identifying the abilities of biostimulants to improve vegetable production compared with conventional fertilizer
Authors: Raza Qurat-Ul-Ain , Rehim Abdur , Bashir Muhammad Amjad , Raza Hafiz Muhammad Ali , Aon Muhammad , Geng Yucong , Moustafa Mahmoud , Alshaharni Mohammed O. , Ali Haider , Lucas Rosa Sanchez
Published: February 2024

Vegetables are important agricultural products with numerous health benefits. Excessive chemical fertilization to meet the food challenge has resulted in environmental and soil health hazards. Considering this aspect, the current study was conducted with the aim to introduce biostimulants as an alternative to chemical fertilizers to improve soil quality, crop quality, and yield. In the first experiment, the response of radish was noted against the application of glycine (GLY), aspartic acid (ASP), lysine (LYS), vitamin B complex (VBC), and chemical fertilizers (CF). The biostimulants were sourced from Sigma Aldrich and Martin Dow Market Ltd. The results indicated that ASP has significantly improved the phenolic contents in shoot (1.01%) and root (12.23%) compared with CF. Additionally, total protein was significantly increased in shoot with GLY (251.81%) and in root with ASP (57.06%). Shoot ascorbic acid contents were significantly improved with ASP (179.90%), VBC (159.91%), and LYS (139.92%). Plant fresh and dry weight was improved with VBC (478.31%) and ASP (364.73%). The N and P concentrations in radish root were higher in VBC (25.93%) and LYS (100%). Soil organic matter was improved ASP (61.51%), followed by VBC (60.13%). Soil available P concentration was also enhanced with LYS (40.43%), ASP (31.20%), and VBC (23.19%). The second experiment was focused on identifying the response of turnip crop against the following treatments: chemical fertilizers (CF), Isabion®(ISA), 25% CF + LYS + GLY (CLG), 25% CF + ASP + GLY (CAG), and 25% CF + ASP + LYS (CAL). The biostimulants were sourced from Sigma Aldrich Syngenta, Pakistan. The results denoted that CAL and ISA significantly improved the phenolic contents in turnip shoot and root. The ascorbic acid in turnip shoot was improved with CAL (19.27%), CAG (18.13%), ISA (17.68%), and in root with CLG (26.96%). The P concentration in turnip shoot was significantly higher in ISA (19.25%), CLG (16.85%), and CAG (12.26%). Soil total N was improved in all treatments. ISA improved the available P concentration, whereas CF (67.87 mg kg−1) followed by ISA (65.93 mg kg−1) improved the soil available K. Both studies conclude that biostimulants capable of improving vegetable quality.
Safety and efficacy revisited: a systematic review and meta-analysis of glue versus tack mesh fixation in laparoscopic inguinal herniorrhaphy
Authors: Raja Sandesh , Raja Adarsh , Ansari Ziyan , Eman Sara , Bajaj Simran , Ahmed Muhammad , Kumar Uday , Shah Yawar Hussain , Jawahar Sachin , Aftab Faisal , Rajani Deepak , Kumar Satesh , Khatri Mahima
Published: February 2024

Background

This analysis addresses the uncertainty surrounding the efficacy of glue mesh fixation (GMF) compared with tack mesh fixation (TMF) in laparoscopic herniorrhaphy. Our meta-analysis incorporates recently conducted randomized controlled trials (RCTs) to enhance the reference for assessing the efficacy and safety of GMF.

Methods

PubMed Central, Google Scholar, Science Direct, and Cochrane Library were extensively reviewed for articles in the English language performed from inception to May 2023 using the keywords “Glue mesh repair,” “Tack mesh repair,” “Inguinal Hernia,” “Herniorrhaphy,” “Laparoscopic,” “Mesh Fixation,” and “Randomized controlled trials.”

Results

In this meta-analysis, we incorporated a total of 20 randomized controlled trials, evaluating each article individually using quality ratings. Compared with TMF, GMF demonstrated a significant reduction in the incidence of chronic pain [RR: 0.40, (0.23, 0.68)] and pain scores on postoperative day 1 [MD: −1.07, (−1.90, −0.25)]. We also used funnel plots and Egger's regression to test for publication bias.

Conclusion

In summary, this meta-analysis establishes the significance of GMF in reducing chronic pain and postoperative day 1 pain compared with TMF. However, no statistically significant difference was noted between the GMF and TMF groups concerning hematoma, seroma, operation time, recurrence rate, and total complications. Nonetheless, given the small number of cases in this study, the findings must be validated in the future by multicenter, large-sample, high-quality RCTs.
Design and Mechanical Properties of Maximum Bulk Modulus Microstructures Based on a Smooth Topology with Grid Point Density
Authors: Zhou Xin , Tao Chenglin , Liang Xi , Liu Zeliang , Li Huijian
Published: February 2024

The aim of topology optimisation is to determine the optimal distribution of material phases within the periodic cells of a microstructure. In this paper, the density of grid points under element volume fraction is constructed to replace the finite elements in the traditional SIMP framework, avoiding jagged and blurry boundaries in the computational process due to grid dependence. This is then combined with homogenisation theory, a microstructure topology optimisation algorithm with maximum bulk modulus under prescribed volume constraints is proposed, which can obtain 2D and 3D topologies with smooth boundaries. In addition, a closed form expression for the two-dimensional topological concave edge structure (taking the most typical topology as an example) was derived, and a compression experiment was conducted on the topological microstructure based on 3D metal printing technology. Scanning electron microscopy showed that the powder bonded on the surface of the printed structure was not completely melted and the step effect caused the finite element analysis results to be higher than the experimental results. Overall, the finite element simulation and experimental results of the concave surface structure have good consistency, with high strength and energy absorption effects. Topologies based on grid point density obtain microstructures with smooth boundaries, and the introduction of the Heaviside smoothing function and multiple filtering steps within this algorithm leads to more robust optimisation, facilitating 3D or 4D printing of microstructures that meet specific design requirements and confirming the feasibility of the proposed topology for lightweighting studies.
An Improved Detection Method for Crop & Fruit Leaf Disease under Real-Field Conditions
Authors: Noon Serosh Karim , Amjad Muhammad , Qureshi Muhammad Ali , Mannan Abdul , Awan Tehreem
Published: February 2024

Using deep learning-based tools in the field of agriculture for the automatic detection of plant leaf diseases has been in place for many years. However, optimizing their use in the specific background of the agriculture field, in the presence of other leaves and the soil, is still an open challenge. This work presents a deep learning model based on YOLOv6s that incorporates (1) Gaussian error linear unit in the backbone, (2) efficient channel attention in the basic RepBlock, and (3) SCYLLA-Intersection Over Union (SIOU) loss function to improve the detection accuracy of the base model in real-field background conditions. Experiments were carried out on a self-collected dataset containing 3305 real-field images of cotton, wheat, and mango (healthy and diseased) leaves. The results show that the proposed model outperformed many state-of-the-art and recent models, including the base YOLOv6s, in terms of detection accuracy. It was also found that this improvement was achieved without any significant increase in the computational cost. Hence, the proposed model stood out as an effective technique to detect plant leaf diseases in real-field conditions without any increased computational burden.
Great Plasticity in a Great Pathogen: Capsular Types, Virulence Factors and Biofilm Formation in ESBL-Producing Klebsiella pneumoniae from Pediatric Infections in Uruguay
Authors: Araújo Lucía , Papa-Ezdra Romina , Ávila Pablo , Iribarnegaray Victoria , Bado Inés , Telechea Hector , Garcia-Fulgueiras Virginia , Vignoli Rafael
Published: February 2024

Klebsiella pneumoniae is widely recognized as an opportunistic hospital and community pathogen. It is one of the priority microorganisms included in the ESKAPE group, and its antibiotic resistance related to extended-spectrum β-lactamases (ESBL) is a global public health concern. The multi-drug resistance (MDR) phenotype, in combination with pathogenicity factors, could enhance the ability of this pathogen to cause clinical infections. The aim of this study was to characterize pathogenicity factors and biofilm formation in ESBL-producing K. pneumoniae from pediatric clinical infections. Capsular types, virulence factors, and sequence types were characterized by PCR. Biofilm formation was determined by a semiquantitative microtiter technique. MDR phenotype and statistical analysis were performed. The K24 capsular type (27%), virulence factors related to iron uptake fyuA (35%) and kfuBC (27%), and sequence types ST14 (18%) and ST45 (18%) were the most frequently detected. Most of the strains were biofilm producers: weak (22%), moderate (22%), or strong (12%). In 62% of the strains, an MDR phenotype was detected. Strains with K24 capsular type showed an association with ST45 and the presence of fyuA; strains with kfuBC showed an association with moderate or strong biofilm production and belonging to ST14. Weak or no biofilm producers were associated with the absence of kfuBC. The MDR phenotype was associated with the main ESBL gene, blaCTX-M-15. The high plasticity of K. pneumoniae to acquire an MDR phenotype, in combination with the factors exposed in this report, could make it even more difficult to achieve a good clinical outcome with the available therapeutics.
A Kinetic Approach to Oxygen Radical Absorbance Capacity (ORAC): Restoring Order to the Antioxidant Activity of Hydroxycinnamic Acids and Fruit Juices
Authors: Asma Umme , Bertotti Maria Letizia , Zamai Simone , Arnold Marcellus , Amorati Riccardo , Scampicchio Matteo
Published: February 2024

This study introduces a kinetic model that significantly improves the interpretation of the oxygen radical absorbance capacity (ORAC) assay. Our model accurately simulates and fits the bleaching kinetics of fluorescein in the presence of various antioxidants, achieving high correlation values (R2 > 0.99) with the experimental data. The fit to the experimental data is achieved by optimizing two rate constants, k5 and k6. The k5 value reflects the reactivity of antioxidants toward scavenging peroxyl radicals, whereas k6 measures the ability of antioxidants to regenerate oxidized fluorescein. These parameters (1) allow the detailed classification of cinnamic acids based on their structure–activity relationships, (2) provide insights into the interaction of alkoxyl radicals with fluorescein, and (3) account for the regeneration of fluorescein radicals by antioxidants. The application of the model to different antioxidants and fruit extracts reveals significant deviations from the results of traditional ORAC tests based on the area under the curve (AUC) approach. For example, lemon juice, rich in ‘fast’ antioxidants such as ascorbic acid, shows a high k5 value, in contrast to its low AUC values. This finding underscores the limitations of the AUC approach and highlights the advantages of our kinetic model in understanding antioxidative dynamics in food systems. This study presents a comprehensive, quantitative, mechanism-oriented approach to assessing antioxidant reactivity, demonstrating a significant improvement in ORAC assay applications.
Halofantrine Hydrochloride Acts as an Antioxidant Ability Inhibitor That Enhances Oxidative Stress Damage to Candida albicans
Authors: Xiong Juan , Wang Li , Feng Zhe , Hang Sijin , Yu Jinhua , Feng Yanru , Lu Hui , Jiang Yuanying
Published: February 2024

Candida albicans, a prominent opportunistic pathogenic fungus in the human population, possesses the capacity to induce life-threatening invasive candidiasis in individuals with compromised immune systems despite the existence of antifungal medications. When faced with macrophages or neutrophils, C. albicans demonstrates its capability to endure oxidative stress through the utilization of antioxidant enzymes. Therefore, the enhancement of oxidative stress in innate immune cells against C. albicans presents a promising therapeutic approach for the treatment of invasive candidiasis. In this study, we conducted a comprehensive analysis of a library of drugs approved by the Food and Drug Administration (FDA). We discovered that halofantrine hydrochloride (HAL) can augment the antifungal properties of oxidative damage agents (plumbagin, menadione, and H2O2) by suppressing the response of C. albicans to reactive oxygen species (ROS). Furthermore, our investigation revealed that the inhibitory mechanism of HAL on the oxidative response is dependent on Cap1. In addition, the antifungal activity of HAL has been observed in the Galleria mellonella infection model. These findings provide evidence that targeting the oxidative stress response of C. albicans and augmenting the fungicidal capacity of oxidative damage agents hold promise as effective antifungal strategies.
Comparative Analysis of the Culture of Pink Shrimp Farfantepenaeus brasiliensis and Pacific White Shrimp Litopenaeus vannamei in Biofloc System
Authors: Krummenauer Dariano , da Silva André Freitas , Xavier Missileny , Foes Geraldo Kipper , Poersch Luís H. , Cardozo Alessandro , Wasielesky Wilson
Published: February 2024

Shrimp farming in the Biofloc Technology System (BFT) is already considered an alternative to the traditional culture. The bioflocs maintain the water quality and can be used as a food supplement for shrimp. The Pacific white shrimp Litopenaeus vannamei forms the basis for most of the production in BFT. However, its culture is limited by the low temperatures. Thus, the BFT culture potential of native species, such as the pink shrimp Farfantepenaeus brasiliensis, should be considered. The present study aimed to compare the cultures of F. brasiliensis and L. vannamei in the grow-out phase in the BFT system. The experiment comprised two treatments: (FB), grown out of F. brasiliensis, and (LV), grown out of L. vannamei. The study lasted 70 days and was conducted at the Marine Station of Aquaculture at the Federal University of Rio Grande, Rio Grande do Sul State, Brazil. The stocking density was 100 shrimp/m² for both species. The shrimp were fed twice a day with commercial food. The physicochemical parameters of the water were monitored throughout the experimental period. The results showed that all physicochemical parameters of the water remained within the tolerated limits for both species. However, during the growth phase in the BFT, it was observed that the L. vannamei shrimp showed a better zootechnical performance than F. brasiliensis. The results indicate that L. vannamei has a higher capacity to catch bioflocs as supplementary food, demonstrating a better response of that species to the BFT system in the grow-out phase compared to F. brasiliensis.
UV Exposure during Cycling as a Function of Solar Elevation and Orientation
Authors: Weihs Philipp , Helletzgruber Sarah , Kranewitter Sofie , Langer Lara , Lumerding Zacharias , Luschin Viktoria , Schmidt Philipp , Heydenreich Jakob , Schmalwieser Alois W.
Published: February 2024

Although cycling is the most prevalent means of locomotion in the world, little research has been done in evaluating the ultraviolet (UV) radiation exposure of cyclists. In this study, a volunteer using a men’s bike was equipped with 10 miniature UV-meters at different body sites. Besides erythemally effective irradiance, the ratio of personal UV exposure to ambient UV radiation was determined for solar elevations up to 65°, taking into account different orientations with respect to the sun. This method provides a universal model that allows for the calculation of UV exposure whenever ambient UV radiation and solar elevation are available. Our results show that the most exposed body sites are the back, forearm, upper arm, and anterior thigh, receiving between 50% and 75% of ambient UV radiation on average. For certain orientations, this percentage can reach 105% to 110%. However, the risk of UV overexposure depends on ambient UV radiation. At lower solar elevations (<40°), the risk of UV overexposure clearly decreases.