bluuebunny/crossref_metadata_2025 huggingface dataset:
Environmental Influences Measured by Epigenetic Clock and Vulnerability Components at Birth Impact Clinical ASD Heterogeneity
Authors: Neri de Souza Reis Viviane , Tahira Ana Carolina , Daguano Gastaldi Vinícius , Mari Paula , Portolese Joana , Feio dos Santos Ana Cecilia , Lisboa Bianca , Mari Jair , Caetano Sheila C. , Brunoni Décio , Bordini Daniela , Silvestre de Paula Cristiane , Vêncio Ricardo Z. N. , Quackenbush John , Brentani Helena
Published: September 2021

Although Autism Spectrum Disorders (ASD) is recognized as being heavily influenced by genetic factors, the role of epigenetic and environmental factors is still being established. This study aimed to identify ASD vulnerability components based on familial history and intrauterine environmental stress exposure, explore possible vulnerability subgroups, access DNA methylation age acceleration (AA) as a proxy of stress exposure during life, and evaluate the association of ASD vulnerability components and AA to phenotypic severity measures. Principal Component Analysis (PCA) was used to search the vulnerability components from 67 mothers of autistic children. We found that PC1 had a higher correlation with psychosocial stress (maternal stress, maternal education, and social class), and PC2 had a higher correlation with biological factors (psychiatric family history and gestational complications). Comparing the methylome between above and below PC1 average subgroups we found 11,879 statistically significant differentially methylated probes (DMPs, p < 0.05). DMPs CpG sites were enriched in variably methylated regions (VMRs), most showing environmental and genetic influences. Hypermethylated probes presented higher rates in different regulatory regions associated with functional SNPs, indicating that the subgroups may have different affected regulatory regions and their liability to disease explained by common variations. Vulnerability components score moderated by epigenetic clock AA was associated with Vineland Total score (p = 0.0036, adjR2 = 0.31), suggesting risk factors with stress burden can influence ASD phenotype.
Meiotic Behavior of Achiasmate Sex Chromosomes in the African Pygmy Mouse Mus mattheyi Offers New Insights into the Evolution of Sex Chromosome Pairing and Segregation in Mammals
Authors: Gil-Fernández Ana , Ribagorda Marta , Martín-Ruiz Marta , López-Jiménez Pablo , Laguna Tamara , Gómez Rocío , Parra María Teresa , Viera Alberto , Veyrunes Frederic , Page Jesús
Published: September 2021

X and Y chromosomes in mammals are different in size and gene content due to an evolutionary process of differentiation and degeneration of the Y chromosome. Nevertheless, these chromosomes usually share a small region of homology, the pseudoautosomal region (PAR), which allows them to perform a partial synapsis and undergo reciprocal recombination during meiosis, which ensures their segregation. However, in some mammalian species the PAR has been lost, which challenges the pairing and segregation of sex chromosomes in meiosis. The African pygmy mouse Mus mattheyi shows completely differentiated sex chromosomes, representing an uncommon evolutionary situation among mouse species. We have performed a detailed analysis of the location of proteins involved in synaptonemal complex assembly (SYCP3), recombination (RPA, RAD51 and MLH1) and sex chromosome inactivation (γH2AX) in this species. We found that neither synapsis nor chiasmata are found between sex chromosomes and their pairing is notably delayed compared to autosomes. Interestingly, the Y chromosome only incorporates RPA and RAD51 in a reduced fraction of spermatocytes, indicating a particular DNA repair dynamic on this chromosome. The analysis of segregation revealed that sex chromosomes are associated until metaphase-I just by a chromatin contact. Unexpectedly, both sex chromosomes remain labelled with γH2AX during first meiotic division. This chromatin contact is probably enough to maintain sex chromosome association up to anaphase-I and, therefore, could be relevant to ensure their reductional segregation. The results presented suggest that the regulation of both DNA repair and epigenetic modifications in the sex chromosomes can have a great impact on the divergence of sex chromosomes and their proper transmission, widening our understanding on the relationship between meiosis and the evolution of sex chromosomes in mammals.
Descending Dysploidy and Bidirectional Changes in Genome Size Accompanied Crepis (Asteraceae) Evolution
Authors: Senderowicz Magdalena , Nowak Teresa , Rojek-Jelonek Magdalena , Bisaga Maciej , Papp Laszlo , Weiss-Schneeweiss Hanna , Kolano Bozena
Published: September 2021

The evolution of the karyotype and genome size was examined in species of Crepis sensu lato. The phylogenetic relationships, inferred from the plastid and nrITS DNA sequences, were used as a framework to infer the patterns of karyotype evolution. Five different base chromosome numbers (x = 3, 4, 5, 6, and 11) were observed. A phylogenetic analysis of the evolution of the chromosome numbers allowed the inference of x = 6 as the ancestral state and the descending dysploidy as the major direction of the chromosome base number evolution. The derived base chromosome numbers (x = 5, 4, and 3) were found to have originated independently and recurrently in the different lineages of the genus. A few independent events of increases in karyotype asymmetry were inferred to have accompanied the karyotype evolution in Crepis. The genome sizes of 33 Crepis species differed seven-fold and the ancestral genome size was reconstructed to be 1C = 3.44 pg. Both decreases and increases in the genome size were inferred to have occurred within and between the lineages. The data suggest that, in addition to dysploidy, the amplification/elimination of various repetitive DNAs was likely involved in the genome and taxa differentiation in the genus.
Formation of Gas-Emission Craters in Northern West Siberia: Shallow Controls
Authors: Chuvilin Evgeny Mikhailovich , Sokolova Natalia Sergeevna , Bukhanov Boris Aleksandrovich , Davletshina Dinara Anvarovna , Spasennykh Mikhail Yurievich
Published: September 2021

Gas-emission craters discovered in northern West Siberia may arise under a specific combination of shallow and deep-seated permafrost conditions. A formation model for such craters is suggested based on cryological and geological data from the Yamal Peninsula, where shallow permafrost encloses thick ground ice and lenses of intra- and subpermafrost saline cold water (cryopegs). Additionally, the permafrost in the area is highly saturated with gas and stores large accumulations of hydrocarbons that release gas-water fluids rising to the surface through faulted and fractured crusts. Gas emission craters in the Arctic can form in the presence of gas-filled cavities in ground ice caused by climate warming, rich sources of gas that can migrate and accumulate under pressure in the cavities, intrapermafrost gas-water fluids that circulate more rapidly in degrading permafrost, or weak permafrost caps over gas pools.
Securing the Limits of Large Ocean States in the Pacific: Defining Baselines Limits and Boundaries amidst Changing Coastlines and Sea Level Rise
Authors: Bernard Leonardo , Petterson Michael , Schofield Clive , Kaye Stuart
Published: September 2021

The Pacific Island States and Territories are traditionally described as ‘small island’ nations. However, they are also ‘large ocean’ nations with jurisdiction over substantial maritime spaces stretching to at least 200 nautical miles. The article addresses issues related to baselines along the coast on which these broad maritime claims depend. The article then examines geodynamic considerations coupled with sea and land level projections in the Pacific leading to an assessment of the vulnerability or resilience of atolls in particular. The article then discusses potential implications for the coastlines and baselines of the Pacific Island States and Territories, the limits of their maritime entitlements and both delimited and undelimited maritime boundaries. Potential response options, prospects, and concluding thoughts are then offered.
Surface and Subsurface Damage Caused by Bullet Impacts into Sandstone
Authors: Campbell Oliver , Blenkinsop Tom , Gilbert Oscar , Mol Lisa
Published: September 2021

The shift of armed conflicts to more urbanised environments has increased the risk to cultural heritage sites. Small arms impacts are ubiquitous in these circumstances, yet the effects and mechanisms of damage caused are not well known. A sandstone target was shot under controlled conditions to investigate surface and subsurface damage. A 3D model of the damaged block, created by structure from motion photogrammetry, shows that internal fracturing was at least as extensive as the visible surface fractures. Backscatter electron imaging of the damaged surface shows a shift from intragranular fracturing and grain size reduction at <5 mm from the impact point to primarily circumgranular fracturing and grain ‘plucking’ at 20 mm from the impact point. Internal fracture intensity decreased with distance from the centre of the crater. Volumes around the impact point are therefore at greater risk of subsequent weathering deterioration, but significant damage extends to the periphery of the target, rendering whole blocks vulnerable. The surface crater, despite being one of the most conspicuous aspects of conflict damage, has many times less area than internal and surface fractures.
An Ecological Study Assessing the Relationship between Public Health Policies and Severity of the COVID-19 Pandemic
Authors: Pasdar Zahra , Pana Tiberiu A. , Ewers Kai D. , Szlachetka Weronika A. , Perdomo-Lampignano Jesus A. , Gamble David T. , Bhattacharya Sohinee , Carter Ben , Myint Phyo K.
Published: September 2021

Reliance on government-led policies have heightened during the COVID-19 pandemic. Further research on the policies associated with outcomes other than mortality rates remains warranted. We aimed to determine associations between government public health policies on the severity of the COVID-19 pandemic. This ecological study including countries reporting ≥25 daily COVID-related deaths until end May 2020, utilised public data on policy indicators described by the Blavatnik school of Government. Associations between policy indicators and severity of the pandemic (mean mortality rate, time to peak, peak deaths per 100,000, cumulative deaths after peak per 100,000 and ratio of mean slope of the descending curve to mean slope of the ascending curve) were measured using Spearman rank-order tests. Analyses were stratified for age, income and region. Among 22 countries, containment policies such as school closures appeared effective in younger populations (rs = −0.620, p = 0.042) and debt/contract relief in older populations (rs = −0.743, p = 0.009) when assessing peak deaths per 100,000. In European countries, containment policies were generally associated with good outcomes. In non-European countries, school closures were associated with mostly good outcomes (rs = −0.757, p = 0.049 for mean mortality rate). In high-income countries, health system policies were generally effective, contrasting to low-income countries. Containment policies may be effective in younger populations or in high-income or European countries. Health system policies have been most effective in high-income countries.
Content and Face Validation of a Novel, Interactive Nutrition Specific Physical Exam Competency Tool (INSPECT) to Evaluate Registered Dietitians’ Competence: A Delphi Consensus from the United States
Authors: Zechariah Sunitha , Waller Jennifer L. , De Leo Gianluca , Stallings Judith , Gess Ashley J. , Lehman Leigh
Published: September 2021

The nutrition-focused physical examination (NFPE) is an integral component of nutrition assessment performed by registered dietitian nutritionists (RDNs) to determine signs of malnutrition and other nutrition-related complications. Increased use of this essential skill among RDNs and the transformation of dietetics education to a competency-based model in the near future calls for appropriately validated tools to measure RDNs’ NFPE competence. To fill the need for a validated competency tool, this study developed an Interactive Nutrition-Specific Physical Exam Competency Tool (INSPECT) utilizing the initial 70 items identified in the first phase of the study. The second phase of this study aimed to test the preliminary version of the INSPECT for content and face validity. An expert panel of 17 members provided consensus recommendations through the Delphi process. Internal consistency of the consensus was measured with Cronbach’s alpha (α) and α of ≥0.70 was defined as acceptable a priori. Inter-rater agreement among the expert panel was determined using the intraclass correlation coefficient (ICC) and an a priori ICC of 0.75 to 0.9 was established as good and >0.9 as excellent agreement. The results showed acceptable face validity (α = 0.71) and excellent content validity for the INSPECT, with an internal consistency of α = 0.97 in the first round and α = 0.96 in the second round. The inter-rater agreement was also excellent with ICC = 0.95 for each of the Delphi rounds. A total of 52 items were retained from the preliminary version of the INSPECT. Open feedback from the experts allowed for the consolidation of 11 similar items for better scoring and evaluation and thus, a total of 41 items were included in the final version of the INSPECT. The final version of the INSPECT is currently being studied in real-life, multi-site clinical settings among practicing RDNs to examine construct validity, reliability, and item-level psychometric properties. Ultimately, the validated INSPECT will be available for the competency evaluation of RDNs practicing in clinical settings.
Analysis of Factors Relevant to Revenue Improvement in Ventral Hernia Repair, Their Influence on Surgical Training, and Development of Predictive Models: An Economic Evaluation
Authors: Taha Anas , Enodien Bassey , Ochs Vincent , Bachmann Marta , Gripp Maike , Adamina Michel , Taha-Mehlitz Stephanie , Frey Daniel
Published: September 2021

Background: Ventral hernia repairs (VHR) are frequent but loss- making. This study aims to identify epidemiological and procedure related factors in VHR and their influence on surgical training. Methods: Data from 86 consecutive patients who underwent VHR in 2019 was collected. Moreover, 66 primary ventral hernias and 20 incisional hernias were repaired in open procedures. Linear regression models were made. Results: Primary VHR procedures showed a mean deficit of −378.17 CHF per case. Incisional hernia repair procedures resulted in a deficit of −1442.50 CHF per case. The two hernia groups were heterogeneous. For the primary VHR procedures, the surgery time (β = 0.564, p < 0.001) had the greatest influence, followed by the costs of the mesh (β = −0.215, p < 0.001). The epidemiological factors gender (β = 0.143, p < 0.01) and body mass index (BMI) (β = −0.087, p = 0.074) were also influential. For incisional hernia procedures a surgeon’s experience had the most significant influence (β = 0.942, p < 0.001), and the second largest influence was the price of the mesh (β = −0.500, p < 0.001). The epidemiological factor BMI (β = −0.590, p < 0.001), gender (β = −0.113, p = 0.055) and age (β = −0.026, p < 0.050) also had a significant influence. Conclusion: Our analysis shows a way of improving financial results in the field of ventral hernia repair. Costs can be visualized and reduced to optimize revenue enhancement in surgical departments. In our analysis primary ventral hernias are an appropriate training operation, in which the experience of the surgeon has no significant impact on costs. In primary VHR procedures, revenue enhancement is limited when using an expensive mesh. However, the treatment of incisional hernias is recommended by specialists. The financial burden is significantly higher with less experience. Therefore, these operations are not suitable for surgical training. The re-operation rate decreases with increasing experience of the surgeon. This directly affects the Patient Related Outcome (PROM) and quality of treatment. Therefore, high-quality training must be enforced. Since financial pressure on hospitals is increasing further, it is crucial to investigate cost influencing factors. The majority of Swiss public hospitals will no longer be able to operate ventral hernias profitably without new concepts. In addition to purchasing management, new construction projects, and mergers, improving the results of individual departments is a key factor in maintaining the profitability of hospitals in the future regarding hernia repair without losing the scope of teaching procedures.
Prevalence and Clustering of Cardiovascular Risk Factors among Medical Staff in Northeast China
Authors: Yu Jianxing , Jia Huanhuan , Zheng Zhou , Cao Peng , Yu Xihe
Published: September 2021

Background: The clustering of cardiovascular disease (CVD) risk factors has become a major public health challenge worldwide. Although many studies have investigated CVD risk factor clusters, little is known about their prevalence and clustering among medical staff in Northeast China. This study aimed to estimate the prevalence and clustering of CVD risk factors and to investigate the association between relevant characteristics and the clustering of CVD risk factors among medical staff in Northeast China. Methods: A cross-sectional survey of 3720 medical staff from 93 public hospitals in Jilin Province was used in this study. Categorical variables were presented as percentages and were compared using the χ2 test. Multiple logistic regression analysis was used to evaluate the association between relevant characteristics and the clustering of CVD risk factors. Results: The prevalence of hypertension, diabetes, dyslipidemia, being overweight, smoking, and drinking were 10.54%, 3.79%, 17.15%, 39.84%, 9.87%, and 21.75%, respectively. Working in a general hospital, male, and age group 18–44 years were more likely to have 1, 2, and ≥3 CVD risk factors, compared with their counterparts. In particular, compared with being a doctor, being a nurse or medical technician was less likely to have 1, 2, and ≥3 CVD risk factors only in general hospitals. Conclusions: The findings suggest that medical staff of general hospitals, males, and older individuals have a high chance associated with CVD risk factor clustering and that more effective interventions should be undertaken to reduce the prevalence and clustering of CVD risk factors, especially among older male doctors who work in general hospitals.
Use of Computer-Based Scenarios for Clinical Teaching: Impact on Nursing Students’ Decision-Making Skills
Authors: Elcokany Nermine M. , Abdelhafez Amal Ismael , Samuel Sharaby Vivian Magdi , Belal Safia
Published: September 2021

Computer-based learning has numerous advantages. It gives students the chance to accommodate and solve problems independently, it can increase motivation during the learning process, and it offers students direct feedback. Students will also receive an authentic learning experience, increasing their level of knowledge retention. It can assist nursing educators in improving learning outcomes. Aim: This study aimed to investigate and evaluate the impact of computer-based scenarios on undergraduate nursing students’ decision-making skills. Sample: There was a total sample of 112 nursing students who were enrolled in a critical care nursing course at the College of Applied Medical Sciences in Saudi Arabia. These students were divided into two groups. Methods: The two groups were taught the same topic for one week. Two case scenarios were given to each group during the clinical rotation. The study group used the computer-based case scenario, and the control group used the paper-based case scenario. The two groups were compared regarding their decision-making skills. The student’s feedback about the computer-based case scenarios was also investigated. Results: The study group scored significantly higher in their decision-making skills when compared to the control group. In addition, the study group reported that they highly agreed that their general learning and specific nursing abilities improved after using computer-based case scenarios.
Dietary Diversity as a Risk Factor for Obesity in Algerian Patients with Type 2 Diabetes Mellitus
Authors: Bounihi Abdenour , Saidi Hamza , Bouazza Asma , Benbaibeche Hassiba , Azzouz Malha , Koceir Elhadj Ahmed
Published: September 2021

Although the incidence of “diabesity” (coexistence of type 2 diabetes and obesity) is alarmingly increasing in Algeria, the diet–diabesity link has not been well defined. This study aimed to explore the association between dietary diversity score (DDS) and obesity among Algerian type 2 diabetic patients. It was a cross-sectional observational study involving 390 type 2 diabetic patients. Anthropometric data were gathered, and dietary intake information was obtained through a 24-h dietary recall method, which was used to calculate DDS. Potential confounders such as age, sex, smoking, physical activity and energy intake were controlled for using multivariate logistic regression. A total of 160 patients (41.3%) were classified as obese. As expected, obese patients had a higher body mass index, waist circumference, hip circumference, body fat and fat mass index. Furthermore, obese patients more frequently met carbohydrate recommendations and had a higher intake of meat and protein. Female sex, hypertension, low physical activity and high meat and protein intake were positively associated with diabesity. Additionally, higher DDS was positively associated with diabesity after adjusting for confounders. Thus, a more diversified diet may be a risk factor for obesity among Algerian type 2 diabetic patients.
Nutrient Solution Temperature Affects Growth and °Brix Parameters of Seventeen Lettuce Cultivars Grown in an NFT Hydroponic System
Authors: Thakulla Dharti , Dunn Bruce , Hu Bizhen , Goad Carla , Maness Niels
Published: September 2021

Nutrient solution temperature in a hydroponic system affects °Brix and yield of lettuce; thus, it is important to maintain the water temperature within an appropriate range. Nutrient-film technique (NFT) trials were conducted to investigate the effects of different water temperatures (18.3 °C, 21.1 °C, and ambient) on growth and °Brix of 17 cultivars from five different types (Loose leaf, Romaine, Butterhead, Salanova, and Batavian) of lettuce. The average daily water temperature for ambient treatment was recorded to be 20 to 26.5 °C. The study was conducted in a split-plot design with three replications over time. Results indicated that water temperature affected root and shoot fresh and dry weight, plant width, and °Brix for lettuce. Lettuce grown at 21.1 °C were 15% greater for shoot fresh weight than plants grown at ambient conditions. All the growth and quality parameters of lettuce were found to be affected by cultivars, with “Coastal Star” showing the best results in both growth and °Brix parameters. All the cultivars of the Romaine type showed greater growth and °Brix, while Salanova lettuce did not perform well in all treatments compared to other lettuce types. For CO2 assimilation, the interaction between water temperature and cultivars was significant, with “Parris Island” having the greatest rate at ambient water temperature. These results suggested that maintaining water temperature at 21.1 °C produced lettuce with greater growth and biomass but had 26% lower °Brix than lettuce grown at 18.3 °C.
Unraveling the Interaction between Arbuscular Mycorrhizal Fungi and Camellia Plants
Authors: Liu Rui-Cheng , Xiao Zhi-Yan , Hashem Abeer , Abd_Allah Elsayed Fathi , Xu Yong-Jie , Wu Qiang-Sheng
Published: September 2021

Camellia is a genus of evergreen shrubs or trees, such as C. japonica, C. sinensis, C. oleifera, etc. A group of beneficial soil microorganisms, arbuscular mycorrhizal fungi (AMF), inhabit the rhizosphere of these Camellia spp. A total of eight genera of Acaulospora, Entrophospora, Funneliformis, Gigaspora, Glomus, Pacispora, Scutellospora, and Sclerocystis were found to be associated with Camellia plants with Glomus and/or Acaulospora being most abundant. These mycorrhizal fungi can colonize the roots of Camellia spp. and thus form arbuscular mycorrhizal symbionts. AMF is an important partner of Camellia spp. in the field of physiological activities. Studies indicated that AMF inoculation has been shown to promote plant growth, improve nutrient acquisition and nutritional quality, and increase resistance to drought, salinity and heavy metal contamination in potted Camellia. This review thus provides a comprehensive overview of AMF species occurring in the rhizosphere of Camellia spp. and summarizes the variation in root AMF colonization rate as well as the environmental factors and soil nutrients affecting root colonization. The paper also reviews the effects of AMF on plant growth response, nutrient acquisition, food quality, and stress tolerance of Camellia spp.
The Putative Role of the NAC Transcription Factor EjNACL47 in Cell Enlargement of Loquat (Eriobotrya japonica Lindl.)
Authors: Chen Qian , Jing Danlong , Wang Shuming , Xu Fan , Bao Chaoya , Luo Ming , Guo Qigao
Published: September 2021

NAC transcription factors (TFs) are plant-specific TFs that play essential roles in plant development; however, the function of NAC TFs in loquat development remains unknown. The natural triploid loquat (Eriobotrya japonica Lindl.), Longquan No.1. B355, has larger organs than its corresponding diploid loquat (B2). Here, we cloned an NAC-like TF (EjNACL47 (NAC-like 47)) from the cDNA of triploid loquat B355 flowers. EjNACL47 has a conserved domain of NAC TFs and is homologous to AtNAC47. Transient expression in tobacco leaves revealed that EjNACL47 localized to the nucleus, and yeast-two-hybrid screening confirmed that the C-terminus displayed transcriptional activity. Interestingly, real-time qRT-PCR indicated that the expression levels of EjNACL47 in leaves and flower organs in triploid loquat (B355) were higher than those in diploid loquat (B2), implying that EjNACL47 might be associated with the larger organ size in B355. Moreover, Arabidopsis lines ectopically expressing EjNACL47 presented obviously larger leaves, flowers, and siliques than the wild-type variant, suggesting that EjNACL47 plays a positive role in Arabidopsis organ enlargement. These results offer insight into the molecular mechanism of NAC TFs involved in regulating organ size in loquat.
Evaluation of Bio-Pesticides against the South American Tomato Leaf Miner, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) in India
Authors: Buragohain Priyakshi , Saikia Dilip Kumar , Sotelo-Cardona Paola , Srinivasan Ramasamy
Published: September 2021

Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most economically important pests of tomato worldwide. Despite its global importance, the management of this oligophagous pest has still been a challenging task, due to its high capability to develop resistance against synthetic insecticides. Given the limited studies on the effectiveness of different bio-pesticides in India, the objective of this research was to determine the pathogenicity of different commercial formulations of Beauveria bassiana, Bacillus thuringiensis, and neem (Azadirachtin), against T. absoluta, under laboratory and field conditions. For the Bacillus thuringiensis formulations, Green Larvicide® and Delfin® recorded an LC50 of 4.10 × 10⁹ CFU/mL and 8.06 × 106 spores/mg, respectively, while for the B. bassiana formulations, Green Beauveria® and BB Power® were 4.473 × 107 spores/mL and 1.367 × 107 CFU/g, respectively. Furthermore, the results showed high susceptibility to both the commercial neem formulations with Ecotin®, recording an LC50 of 91.866 ppm, and Econeem Plus® recording 212.676 ppm. The results from the field conditions at different locations of Andhra Pradesh, India, showed significant differences (p < 0.001) for leaf and fruit infestation among the interaction effect of treatments and locations. Bio-pesticides, especially neem and B. thuringiensis formulations, reduced T. absoluta infestation similarly to the chemical treatment, without affecting the yield. Therefore, bio-pesticides can be considered as safe alternatives to synthetic pesticides, for the management of T. absoluta.
Interstitial Ectopic Pregnancy: The Role of Mifepristone in the Medical Treatment
Authors: Stabile Guglielmo , Romano Federico , Zinicola Giulia , Topouzova Ghergana Alexandrova , Di Lorenzo Giovanni , Mangino Francesco Paolo , Ricci Giuseppe
Published: September 2021

Interstitial pregnancy is defined as the presence of a gestational sac in the most proximal section of the fallopian tube. Management of interstitial pregnancy remains a debated topic. Depending on hemodynamic stability, size of pregnancy, depth of surrounding myometrium, and desires for future fertility, interstitial pregnancy can be managed medically or surgically. We reviewed the literature in December 2020 using keywords “interstitial pregnancy”, “medical treatment”, “methotrexate”, and “mifepristone”. Articles published from January 1991 until 2020 were obtained from databases EMBASE, SCOPUS, and PUBMED. We describe the case of a patient with an interstitial pregnancy that was managed with a total medical approach in August 2020 at Burlo Garofolo Hospital. The patient was asymptomatic and hemodynamically stable, with a high level of serum β-hCG (22,272 mUi/mL). We used the combination of methotrexate (MTX) and mifepristone. Medical therapy was effective leading to interstitial pregnancy resolution in 51 days without collateral effects for the patient. We found seven previous cases reported in the literature. Our purpose is to underline the efficacy of medical therapy with systemic multidose MTX associated with a single oral dose of mifepristone and also folinic acid when is present a viable fetus and a high serum β-hCG level.
Diverse Manifestations of COVID-19: Some Suggested Mechanisms
Authors: Zaman Md S. , Sizemore Robert C.
Published: September 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the novel respiratory disease COVID-19, has reached pandemic status and presents a wide range of manifestations of diverse magnitude, including fever, cough, shortness of breath, and damage to vital organs, such as the heart, lung, kidney, and brain. Normally, older individuals and those with underlying health issues are more at risk. However, about 40% of COVID-19 positive individuals are asymptomatic. This review aims to identify suggested mechanisms of diverse manifestations of COVID-19. Studies suggest that T cell-mediated immunity and specific and/or nonspecific immunity from other vaccines could protect against SARS-CoV-2. The potential role of cross-reacting antibodies to coronaviruses that cause the common cold, mumps virus, polio virus, and pneumococcal bacteria are also suggested to help protect against COVID-19. Decreased production of Type I interferons (IFN-α and IFN-β) could also be linked to COVID-19 manifestations. Several studies suggest that ACE2 cell membrane receptors are involved in SARS-CoV-2 infection. However, the relationship between an abundance of ACE2 receptors and the infectivity of the virus is unknown. Unlocking these manifestation mysteries could be crucial as this could help researchers better understand the virulence, pathology, and immune responses associated with SARS-CoV-2, leading to the development of effective therapies and treatment plans.
Use and Significance of Nursing Diagnosis in Hospital Emergencies: A Phenomenological Approach
Authors: Cachón-Pérez Jose Miguel , Gonzalez-Villanueva Purificación , Rodriguez-Garcia Marta , Oliva-Fernandez Oscar , Garcia-Garcia Esther , Fernandez-Gonzalo Juan Carlos
Published: September 2021

Background: Professional nursing organizations recommend the use of nursing diagnosis to enhance and facilitate the standardization of care and the development of a common language used by nursing practitioners. In the clinical reality of hospital emergency departments, however, its use is controversial. The objectives of the research are (a) to explore the use of nursing diagnosis in hospital emergency departments, and (b) to describe the meaning of nursing diagnosis for hospital emergency nurses. Methods: A qualitative phenomenological study was conducted. A purposeful sampling and snowball technique were used. Data were collected using in-depth interviews, researchers’ field notes, and documental analysis. An inductive analysis based on Giorgi´s proposal was used to identify significant emerging themes from interviews and field notes. Seventeen participants with a mean age of 40 were recruited. Results: Three themes were identified. The results showed how the use of nursing diagnosis in hospital emergency departments depends on nurses to apply a working methodology in their practice, along with other dimensions such as the characteristics of emergency care, the type of health problems, and the complexity of care. Conclusions: The use of standardized language in emergency departments is complex due to the overcrowded nature of care in these settings.
Ground Reaction Force Differences between Bionic Shoes and Neutral Running Shoes in Recreational Male Runners before and after a 5 km Run
Authors: Jiang Xinyan , Zhou Huiyu , Quan Wenjing , Hu Qiuli , Baker Julien S. , Gu Yaodong
Published: September 2021

Running-related injuries are common among runners. Recent studies in footwear have shown that designs of shoes can potentially affect sports performance and risk of injury. Bionic shoes combine the functions of barefoot running and foot protection and incorporate traditional unstable structures based on bionic science. The purpose of this study was to investigate ground reaction force (GRF) differences for a 5 km run and how bionic shoes affect GRFs. Sixteen male recreational runners volunteered to participate in this study and finished two 5 km running sessions (a neutral shoe session and a bionic shoe session). Two-way repeated-measures ANOVAs were performed to determine the differences in GRFs. In the analysis of the footwear conditions of runners, bionic shoes showed significant decreases in vertical impulse, peak propulsive force, propulsive impulse, and contact time, while the braking impulse and vertical instantaneous loading rate (VILR) increased significantly compared to the neutral shoes. Main effects for a 5 km run were also observed at vertical GRFs and anterior–posterior GRFs. The increases of peak vertical impact force, vertical average loading rate (VALR), VILR, peak braking force and braking impulse were observed in post-5 km running trials and a reduction in peak propulsive force and propulsive impulse. The interaction effects existed in VILR and contact time. The results suggest that bionic shoes may benefit runners with decreasing injury risk during running. The findings of the present study may help to understand the effects of footwear design during prolonged running, thereby providing valuable information for reducing the risk of running injuries.
A Review of the Preservation of Hard and Semi-Hard Cheeses: Quality and Safety
Authors: Nájera Ana Isabel , Nieto Sonia , Barron Luis Javier R. , Albisu Marta
Published: September 2021

Cheese is a dairy product with potential health benefits. Cheese consumption has increased due to the significant diversity of varieties, versatility of product presentation, and changes in consumers’ lifestyles. Spoilage of hard and semi-hard cheeses can be promoted by their maturation period and/or by their long shelf-life. Therefore, preservation studies play a fundamental role in maintaining and/or increasing their shelf-life, and are of significant importance for the dairy sector. The aim of this review is to discuss the most effective methods to ensure the safety and sensory quality of ripened cheeses. We review traditional methods, such as freezing, and modern and innovative technologies, such as high hydrostatic pressures, chemical and natural vegetable origin preservatives, vacuum and modified atmosphere packaging, edible coatings and films, and other technologies applied at the end of storage and marketing stages, including light pulses and irradiation. For each technology, the main advantages and limitations for industrial application in the dairy sector are discussed. Each type of cheese requires a specific preservation treatment and optimal application conditions to ensure cheese quality and safety during storage. The environmental impact of the preservation technologies and their contribution to the sustainability of the food chain are discussed.
Incarceration and COVID-19: Recommendations to Curb COVID-19 Disease Transmission in Prison Facilities and Surrounding Communities
Authors: Natoli Lauren Jeanne , Vu Kathy Linh , Sukhija-Cohen Adam Carl , Engeran-Cordova Whitney , Maldonado Gabriel , Galvin Scott , Arroyo William , Davis Cynthia
Published: September 2021

Overcrowding can increase the risk of disease transmission, such as that of SARS-CoV-2 (COVID-19), within United States prisons. The number of COVID-19 cases among prisoners is higher than that among the general public, and this disparity is further increased for prisoners of color. This report uses the example case of the COVID-19 pandemic to observe prison conditions and preventive efforts, address racial disparities for people of color, and guide structural improvements for sustaining inmate health during a pandemic in four select states: California, New York, Illinois, and Florida. To curb the further spread of COVID-19 among prisoners and their communities, safe public health practices must be implemented including providing personal protective equipment (PPE) and testing of staff and inmates, disseminating culturally and language appropriate information regarding the pandemic and preventive precautions, introducing social distancing measures, and ensuring adequate resources to safely reintegrate released prisoners into their communities.
Sugars and Sweet Taste: Addictive or Rewarding?
Authors: Greenberg Danielle , St. Peter John V.
Published: September 2021

The notion of food “addiction” often focuses on the overconsumption of sweet tasting foods or so-called sugar “addiction”. In the extreme, some have suggested that sugar and sweet tastes elicit neural and behavioral responses analogous to those observed with drugs of abuse. These concepts are complicated by the decades long uncertainty surrounding the validity and reproducibility of functional magnetic resonance imaging (fMRI) methodologies used to characterize neurobiological pathways related to sugar and sweet taste stimuli. There are also questions of whether sweet taste or post-ingestion metabolic consequences of sugar intake would lead to addiction or excessive caloric intake. Here, we present a focused narrative review of literature related to the reward value of sweet taste which suggests that reward value can be confounded with the construct of “addictive potential”. Our review seeks to clarify some key distinctions between these constructs and questions the applicability of the addiction construct to human over-eating behaviors. To adequately frame this broad discussion requires the flexibility offered by the narrative review paradigm. We present selected literature on: techniques used to link sugar and sweet tastes to addiction neurobiology and behaviors; sugar and sweet taste “addiction”; the relationship of low calorie sweetener (LCS) intake to addictive behaviors and total calorie intake. Finally, we examined the reward value of sweet tastes and contrasted that with the literature describing addiction. The lack of reproducibility of fMRI data remains problematic for attributing a common neurobiological pathway activation of drugs and foods as conclusive evidence for sugar or sweet taste “addiction”. Moreover, the complicated hedonics of sweet taste and reward value are suggested by validated population-level data which demonstrate that the consumption of sweet taste in the absence of calories does not increase total caloric intake. We believe the neurobiologies of reward value and addiction to be distinct and disagree with application of the addiction model to sweet food overconsumption. Most hypotheses of sugar “addiction” attribute the hedonics of sweet foods as the equivalent of “addiction”. Further, when addictive behaviors and biology are critically examined in totality, they contrast dramatically from those associated with the desire for sweet taste. Finally, the evidence is strong that responses to the palatability of sweets rather than their metabolic consequences are the salient features for reward value. Thus, given the complexity of the controls of food intake in humans, we question the usefulness of the “addiction” model in dissecting the causes and effects of sweet food over-consumption.
Stability of Class II Malocclusion Treatment with the Austro Repositioner Followed by Fixed Appliances in Brachyfacial Patients
Authors: Austro-Martinez Maria Dolores , Nicolas-Silvente Ana I. , Velasco-Ortega Eugenio , Jimenez-Guerra Alvaro , Alarcon Jose A.
Published: September 2021

One of the goals of functional-appliance devices is to modify the vertical growth pattern, solving several kinds of malocclusion. This study aimed to evaluate Class II malocclusion treatment’s stability with Austro Repositioner, followed by fixed appliances, and assess its capacity to modify vertical dimensions in brachyfacial patients. A test group of 30 patients (16 boys and 14 girls, mean 11.9 years old) with Class II malocclusion due to mandibular retrognathism and brachyfacial pattern treated with Austro Repositioner and fixed appliance were compared to a matched untreated Class II control group of 30 patients (17 boys and 13 girls, mean age 11.7 years old). Lateral cephalograms were taken at T1 (initial records), T2 (end of treatment), and T3 (one year after treatment). Statistical comparisons were performed with a paired-sample t-test and two-sample t-tests. Significant improvements in the skeletal Class II relationship were observed in the treated group. The ANB angle decreased (4.75°), the SNB angle increased (3.92°), and the total mandibular length (Co-Pg) increased (8.18 mm) (p < 0.001). Vertical dimensions were also significantly modified, the FMA angle increased (3.94°), LAFH-distance increased (3.15 mm), and overbite decreased (3.35 mm). These changes remained stable one year after treatment. The Austro Repositioner was adequate for treating the skeletal Class II malocclusion resulting from the mandible retrusion in brachyfacial patients.
Does the Consumer Sociodemographic Profile Influence the Perception of Aspects Related and Not Related to Food Safety? A Study in Traditional Spanish Street Markets
Authors: Verdú Abel , Millán Rafael , Saavedra Pedro , Iruzubieta Conrado Javier Carrascosa , Sanjuán Esther
Published: September 2021

Street food markets are important for local economic development, but they must also meet visitors’ demands while operating. Since consumers’ trust is based on their perception on different aspects of these markets, the aim of this work was to study which factors most affected their purchase decision criteria. A total of 950 surveys were collected in 21 street markets (Canary Islands, Spain), recording data from the consumers’ estimation on food safety-related items (i.e., hygiene conditions of market installations, products, and food handlers) as well as other categories (i.e., prices and staff professionalism). The gathered data let us determine whether sociodemographic consumers variables like age, gender, or education level influenced their perceptions. The scores showed a strong correlation, the subsequent principal components analysis explained 81% of variability only with the first two components. The level of tolerance toward all items underlies in the first component, which was significantly higher for those aged 60 and older, but no significant correlation was found for gender or level of education. The youngest participants were more demanding about food safety-related aspects, while the middle-aged group was more critical of prices. This was especially true of females, who demanded better quality:price ratios. Knowing these preferences could facilitate the development of more effective marketing strategies, helping make street markets more competitive.
Moral Identity and Subjective Well-Being: The Mediating Role of Identity Commitment Quality
Authors: Cui Peng , Mao Yanhui , Shen Yufan , Ma Jianhong
Published: September 2021

Moral identity is associated with people’s subjective well-being; however, little is known about how an individual with moral identity relates to one’s subjective well-being. Based on the eudaimonic identity theory, the current study proposed that identity commitment quality is a critical mechanism that links moral identity (two dimensions: internalization and symbolization) and subjective well-being. We examined our hypotheses in 419 college students, who completed the Self-importance of Moral Identity Questionnaire, Satisfaction with Life Scale, Scale of Positive and Negative Experience, and Questionnaire for Eudaimonic Well-being. Results confirmed significant positive correlations among moral identity, identity commitment quality, and subjective well-being; findings also suggested that both the internalization and symbolization dimensions of moral identity predicted subjective well-being through identity commitment quality, and identity commitment quality fully mediated the pathway relationship between moral identity and subjective well-being. We discussed these findings with respect to implications and proposed research suggestions for future studies.
Correction: Shrader-Frechette, K.; Biondo, A.M. Health Misinformation about Toxic-Site Harm: The Case for Independent-Party Testing to Confirm Safety. Int. J. Environ. Res. Public Health 2021, 18, 3882
Authors: Shrader-Frechette Kristin , Biondo Andrew M.
Published: September 2021

Because part of the text was unintentionally omitted, the first paragraph under Section 2.2.4.3. on p. 13 was jumbled and incomplete when it was published [...]
Cardiorespiratory Fitness and Muscular Strength Moderates the Relationship between FNDC5 Polymorphism and Adiposity in Children and Adolescents
Authors: Todendi Pâmela Ferreira , Brand Caroline , de Castro Silveira João Francisco , Burns Ryan Donald , Martínez J. Alfredo , Fiegenbaum Marilu , Reis Gaya Anelise , Pollo Renner Jane Dagmar , Reuter Cézane Priscila , de Moura Valim Andréia Rosane
Published: September 2021

The human locus FNDC5 rs16835198 contributes positively to anthropometric phenotypes in children and adolescents. However, the role of specific components of physical fitness in this relationship is not known. The present study aimed to verify the moderator role of cardiorespiratory fitness (CRF) and muscular strength in the relationship between rs16835198 polymorphism FNDC5 and adiposity in children and adolescents. This cross-sectional study was carried out by genotyping the rs16835198 FNDC5 polymorphism in 1701 children and adolescents (mean age 11.73 ± 2.75 years). Obesity was assessed using waist circumference and body mass index (BMI) z-scores. To evaluate CRF and muscular strength, the 6 min run/walk test and lower limb strength (LLS) were used. Linear regression models were applied, and all analyses were adjusted for age, sex, skin color, living area, and school type. A significant interaction term for CRF (p = 0.038) and LLS (p = 0.040) × rs16835198 FNDC5 with WC was identified. Regarding BMI, a significant interaction term for CRF (p = 0.007) and LLS (p = 0.044) × rs16835198 FNDC5 was observed. Moreover, medium and high CRF and LLS levels protected against higher WC and BMI. In conclusion, adiposity levels of children and adolescents with a genetic predisposition to obesity might be modified by improving CRF and muscular strength.