bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Graphene Nanoplatelets as Lubricant Additive on Fuel Consumption During Vehicle Emission Tests
Authors: Tomanik Eduardo , Christinelli Wania , Garcia Pamela Sierra , Rajala Scott , Crepaldi Jesuel , Franzosi Davi , Souza Roberto Martins , Rovai Fernando Fusco
Published: January 2025

Lubricant friction modifier additives are used on lower viscosity engine oils to mitigate boundary friction. This work presents the development of a graphene-based material as an oil friction modifier additive, from formulation to actual vehicle tests. The graphene material was initially characterized using scanning electron microscopy (SEM) and Raman spectroscopy, which revealed the predominance of graphene nanoplatelets (GNPs) with an average of nine layers. After functionalization, two GNP additive variants were initially mixed with a fully formulated SAE 0W-20 engine oil and tribologically evaluated using reciprocating sliding tests at 40 and 120 °C and Hertzian pressure up to 1.2 GPa when both variants presented friction reduction. Then, the GNP additive variant with better performance was evaluated in a vehicle emission test using a fully formulated 5W-20 SAE oil as a reference. The addition of 0.1% of GNPs reduced fuel consumption by 2.6% in urban conditions and 0.8% in highway ones. The urban test cycle was FTP75 and higher benefits of the GNP additive occurred especially on the test start, when the engine and oil were still cold and on test portions where the vehicle speed was lower.
Biobased Strategies for E-Waste Metal Recovery: A Critical Overview of Recent Advances
Authors: Ferreira-Filipe Diogo A. , Duarte Armando C. , Hursthouse Andrew S. , Rocha-Santos Teresa , Silva Ana L. Patrício
Published: January 2025

The increasing e-waste volumes represent a great challenge in the current waste management landscape, primarily due to the massive production and turnover of electronic devices and the complexity of their components and constituents. Traditional strategies for e-waste treatment focus on metal recovery through costly, energetically intensive, and environmentally hazardous processes, such as pyrometallurgical and hydrometallurgical approaches, often neglecting other e-waste constituents. As efforts are directed towards creating a more sustainable and circular economic model, biobased alternative approaches to these traditional techniques have been increasingly investigated. This critical review focuses on recent advances towards sustainable e-waste treatment, exclusively considering studies using e-waste sources. It addresses, from a critical perspective, approaches using inactive biomass, live biomass, and biogenic compounds, showcasing the diversity of strategies and discussing reaction parameters, advantages and disadvantages, challenges, and potential for valorization of generated by-products. While ongoing research focuses on optimizing operational times and metal recovery efficiencies, bioprocessing approaches still offer significant potential for metal recovery from e-waste. These approaches include lower environmental impact by reducing energy consumption and effluent treatments and the ability to recover metals from complex e-waste streams, paving the way for a more circular economy in the electronics industry.
Influence of Thermal Treatment on Properties of Ash Wood
Authors: Lunguleasa Aurel , Spirchez Cosmin
Published: January 2025

The objective of this study was to investigate some of the properties of ash (Fraxinus excelsior L.) wood treated at a temperature of 185 °C for 3 h. We tested the samples’ physical properties, including their dimensional stability in the form of water absorption and radial/tangential swelling and their surface roughness, as well as their mechanical characteristics such as Brinel hardness and bending strength. Our results show that the water absorption and swelling values of the samples improved, ranging from 27% to 32% and from 21% to 46%, respectively. Also, the Brinell hardness of the thermally treated wood registered a slight decrease from 19.5 N/mm2 to 15.2 N/mm2 in the radial direction and from 15.7 N/mm2 to 12.2 N/mm2 in the tangential direction. It appears that the bending resistance of the samples decreased, which is generally thought to be an adverse effect of heat treatment on any wood species.
Altitude and Stem Height Position as Determinants of the Hydrological Properties of Norway Spruce Bark
Authors: Ilek Anna , Tonello Kelly Cristina
Published: January 2025

Tree bark plays a crucial role in the distribution of rainfall within forest ecosystems, particularly through its impact on stemflow. To gain a comprehensive understanding of how bark controls stemflow, it is essential to identify all factors affecting bark water storage capacity, as this determines the onset of stemflow during rainfall events. Our study analyzed how the position of bark on the stem and the altitude above sea level impact bulk density, water storage capacity, and the time required for bark saturation. We conducted research on Norway spruce bark collected at four altitudes: 400, 550, 700, and 1150 m asl. Our findings revealed that bark from the 400 m altitude had a bulk density that was approximately 24.5% greater than that from higher altitudes. Additionally, the water absorption time for bark from 1150 m was over 68% longer than that for bark from other altitudes. The longest absorption time (about 6.4 days) was observed in the bottom part of the trees, while the shortest (about 4.4 days) was in the top part of the trees. We also observed that the bark water storage capacity increased from the base to the top of the trees and with increasing altitudes. Specifically, the water storage capacity of bark taken from 400 m was approximately 33% lower than that from 1150 m. These findings highlight the significance of stem height position and altitude as key determinants of bark water storage capacity.
Alleviating Microbial Carbon Limitation in Pinus armandii Forests Through Panax notoginseng Cultivation
Authors: Rui Rui , Hei Jingying , Li Yue , Wan Xiaoli , Wang Shu , He Xiahong
Published: January 2025

The cultivation of Panax notoginseng (Sanqi) within the Pinus armandii forest understory has been widely promoted in Yunnan, China. However, relatively little is known about how Sanqi cultivation influences microbial metabolic limitations and their driving factors in P. armandii ecosystems in terms of soil extracellular enzyme activity (EEA) and stoichiometry (EES). In this study, we established monoculture P. armandii (MPA) and Sanqi–P. armandii agroforestry (SPA) systems to investigate microbial resource limitations in P. armandii soils over 12 months (semi-monthly sampling). Sanqi cultivation decreased EEAs in P. armandii soils in the SPA system. Moreover, the vector length in both the bulk and rhizosphere soils of P. armandii decreased significantly from 1.31 to 1.12 and 1.29 to 1.21, respectively, indicating a decrease in the microbial C limitation of P. armandii soils. A vector angle < 45° in both systems revealed that N, rather than P, predominantly restricted microbial metabolism. The most influential factors affecting vector length and angle were Sanqi cultivation and seasonal dynamics. Structural equation modelling (SEM) revealed that fungi-to-bacteria ratios and soil chemical properties were direct factors positively affecting vector length. Overall, our findings suggest that Sanqi cultivation benefited soil microorganisms in P. armandii soils and should be encouraged to supply N to promote the sustainable development of P. armandii.
Spatial Distribution Pattern and Factors Influencing the Endangered Plant Tetracentron sinense Oliv.
Authors: Chen Rui , Zhang Xuemei , Shu Yumin , Liu Qinsong , Zhang Jun , Han Hongyan , Gan Xiaohong
Published: January 2025

Tetracentron sinense is a tall deciduous tree and represents the only remaining species of Tetracentron. Currently, the spatial distribution pattern of T. sinense and its associated influencing factors remain unclear, thus hindering its protection and rational utilization. In this study, we employed the point pattern method to analyze the spatial distribution patterns of four representative populations of T. sinense distributed in Baima Snow Mountain, Dafengding, Leigong Mountain, and Foping in China. The results reveal that the T. sinense populations in Baima Snow Mountain, Dafengding, and Leigong Mountain exhibited an aggregated distribution on small (0–10 m) or specific scales, with their spatial distribution patterns shifting from aggregated to random as the scale increased. In contrast, the population of T. sinense in Foping showed a random distribution at all scales. In relation to the factors influencing the spatial distribution patterns of T. sinense, we found that young trees played a crucial role and had a substantial impact on their distribution. Furthermore, adult trees contributed to the aggregated distribution of T. sinense saplings on smaller scales (0–10 m). Additionally, we identified Acer erianthum, Prunus conradinae, and Rhododendron anthosphaerum as key associated species that influenced the formation of spatial distribution patterns of T. sinense. Finally, air humidity and soil moisture content were found to exert a significant effect on the spatial distribution patterns of T. sinense populations. For the protection of T. sinense in situ, it is necessary to increase the number of young trees, enhance the availability of microhabitat factors for its seedlings, and utilize key companion species to promote heterogeneity, which can optimize resource utilization and foster population rejuvenation.
Expression of Secondary Sexual Dimorphism in the Diurnal Course of Leaf Gas Exchanges Is Modified by the Rhythmic Growth of Ilex paraguariensis Under Monoculture and Agroforestry
Authors: Rakočević Miroslava , Batista Eunice Reis , de Almeida Rafael Leonardo , Wendling Ivar , Ribeiro Rafael Vasconcelos
Published: January 2025

Dioecious species show a division of labor expressed through the differentiated manifestation of resource acquisition. We hypothesized that the expression of secondary sexual dimorphism (SSD) in the leaf gas exchange of yerba mate would be more intensive in females than in males to permit females the carbon investments necessary to finish the reproductive cycle. This species can present two growth units annually (GU1-fall and GU2-spring) intercalated with two rest periods (R1-summer and R2-winter). The leaf area index (LAI) and the diurnal courses of leaf photosynthesis (Anet), stomatal conductance (gs), leaf transpiration (E), intercellular CO2 concentration (Ci), water use efficiency (WUE), and instantaneous carboxylation efficiency (Anet/Ci) were estimated in female and male plants of yerba mate during four periods of annual rhythmic growth in monoculture (MO) and agroforestry (AFS). Leaf gas exchanges varied over the annual rhythmic growth and were more intensive under MO than under AFS. Anet, Anet/Ci ratios, and WUE were higher in females than in males during the summer (R1) and spring (GU2). Also, gs and E were more intensive in females than males during the summer. Oppositely, higher WUE in males than in females was observed during the fall (GU1) and winter (R2), with males also showing a higher Anet/Ci ratio during the winter and higher E during the spring (GU2). Despite the strong effect of the cultivation system on LAI and leaf gas exchange traits over the diurnal course, SSD expression was rarely modified by the cultivation system, being expressed only in MO for E during the spring (GU2) and WUE during the winter (R2). High WUE in males during the winter would benefit plants during cold and dry periods, improving the balance between carbon acquisition and water loss through transpiration. On the other hand, high Anet during the summer and spring could be considered as a general fitness strategy of female plants to improve photoassimilate supply and support their additional reproduction costs.
MultiProduct Optimization of Cedrelinga cateniformis (Ducke) Ducke in Different Plantation Systems in the Peruvian Amazon
Authors: Baselly-Villanueva Juan Rodrigo , Fernández-Sandoval Andrés , Salazar-Hinostroza Evelin Judith , Cárdenas-Rengifo Gloria Patricia , Puerta Ronald , Trigoso Tony Steven Chuquizuta , Rufasto-Peralta Yennifer Lisbeth , Vallejos-Torres Geomar , Casas Gianmarco Goycochea , Araújo Junior Carlos Alberto , Quiñónez-Barraza Gerónimo , Álvarez-Álvarez Pedro , Leite Helio Garcia
Published: January 2025

This study addressed multi-product optimization in Cedrelinga cateniformis plantations in the Peruvian Amazon, aiming to maximize volumetric yields of logs and sawn lumber. Data from seven plantations of different ages and types, established on degraded land, were analyzed by using ten stem profile models to predict taper and optimize wood use. In addition, the structure of each plantation was evaluated using diameter distributions and height–diameter ratios; log and sawn timber production was optimized using SigmaE 2.0 software. The Garay model proved most effective, providing high predictive accuracy (adjusted R2 values up to 0.963) and biological realism. Marked differences in volumetric yield were observed between plantations: older and more widely spaced plantations produced higher timber volumes. Logs of optimal length (1.83–3.05 m) and larger dimension wood (e.g., 25.40 × 5.08 cm) were identified as key contributors to maximizing volumetric yields. The highest yields were observed in mature plantations, in which the total log volume reached 508.1 m3ha−1 and the sawn lumber volume 333.6 m3ha−1. The findings demonstrate the power of data-driven decision-making in the timber industry. By combining precise modeling and optimization techniques, we developed a framework that enables sawmill operators to maximize log and lumber yields. The insights gained from this research can be used to improve operational efficiency and reduce waste, ultimately leading to increased profitability. These practices promote support for smallholders and the forestry industry while contributing to the long-term development of the Peruvian Amazon.
Heterologous Expression and Functional Analysis of Exiguobacterium Algin Lyase Gene by Pichia pastoris
Authors: Wu Hanwen , Hou Kai , Jiang Yutong , Luan Mingjian , Sun Yuxia , He Xi , Zhao Xiangzhong
Published: January 2025

Algin is the most abundant substance in alga. Alginate lyase degrades algin and produces algin monosaccharides, disaccharides, and oligosaccharides, which are widely used in bioenergy, food, medicine, and other fields. In this study, one Exiguobacterium strain isolated from rotten kelp exhibited a robust ability to degrade the alga. The sequencing of this strain revealed the presence of three different types of algin alginate lyase. Nevertheless, the expression of three genes in Escherichia coli revealed a lower alginate lyase activity compared to that of the original strain. After codon optimization, the gene with the highest activity of the three was successfully expressed in Pichia pastoris to produce recombinant EbAlg664. The activity of the recombinant enzyme in 5 L high-density fermentation reached 1306 U/mg protein, 3.9 times that of the original Exiguobacterium strain. The results of the enzymatic analysis revealed that the optimal temperature and the pH range of recombinant EbAlg664 were narrower compared to the original strain. Additionally, the presence of Cu2+ and Co2+ enhanced the enzymatic activity, whereas Mg2+ and Fe3+ exhibited inhibitory effects on the recombinant alginate lyase. The study offers a theoretical and practical foundation for the industrial-scale production of engineered Pichia pastoris with high alginate lyase activity.
Impact of Commercial Protective Culture on Manouri PDO Cheese
Authors: Bintsis Thomas , Kyritsi Maria A.
Published: January 2025

Manouri is a Greek whey cheese, with a Protected Denomination of Origin recognition, produced by heating the cheese whey and added milk and/or cream at high temperatures (88–90 °C) to form a coagulum. High-heat treatment results in the inactivation of any indigenous microorganisms from the raw materials. However, the high moisture, fat and pH of the cheese make it a favorable medium for the growth of any microbial contamination. The objective of this study was to investigate the application of a commercial protective culture (CPC) on the microbial profile of Manouri cheese during storage. Three treatments were prepared: S1 was the control cheese with sterile water sprayed on the surface, S2 was sprayed with a dense CPC and S3 with a diluted CPC. The experimental cheeses were covered with greaseproof paper and stored at 5 °C for 21 days. For all three treatments, the fat content and total solids showed a significant increase during storage, while protein and carbohydrates showed a significant decrease at 14 days. The application of the CPC resulted in cheeses with higher pH than the control cheese, probably due to the growth of acidifying lactic acid bacteria in the microbiota of the S2 and S3 cheeses. Manouri cheese sprayed with the CPC showed a reduction of 1.60–1.69 log CFU/g in the population of yeasts; no effect was observed on Enterobacteriaceae and Staphylococcus spp. The dominant yeast microbiota was identified as Candida zeylanoides (63.5%), Candida parapsilosis (21.1%) and Candida famata (15.4%). Although the application of the CPC was not able to control the spoilage bacteria, it showed an effective way to control the growth of yeasts in Manouri cheese. However, the presence of certain Candida spp. reveals the significance of applying good hygiene practices throughout the cheesemaking process.
Resource Assignment Algorithms for Autonomous Mobile Robots with Task Offloading
Authors: Baruffa Giuseppe , Rugini Luca
Published: January 2025

This paper deals with the optimization of the operational efficiency of a fleet of mobile robots, assigned with delivery-like missions in complex outdoor scenarios. The robots, due to limited onboard computation resources, need to offload some complex computing tasks to an edge/cloud server, requiring artificial intelligence and high computation loads. The mobile robots also need reliable and efficient radio communication with the network hosting edge/cloud servers. The resource assignment aims at minimizing the total latency and delay caused by the use of radio links and computation nodes. This minimization is a nonlinear integer programming problem, with high complexity. In this paper, we present reduced-complexity algorithms that allow to jointly optimize the available radio and computation resources. The original problem is reformulated and simplified, so that it can be solved by also selfish and greedy algorithms. For comparison purposes, a genetic algorithm (GA) is used as the baseline for the proposed optimization techniques. Simulation results in several scenarios show that the proposed sequential minimization (SM) algorithm achieves an almost optimal solution with significantly reduced complexity with respect to GA.
Fire Hazards Caused by Equipment Used in Offshore Oil and Gas Operations: Prescriptive vs. Goal-Oriented Legislation
Authors: Brkić Dejan
Published: January 2025

This article offers a concise overview of the best practices for safety in offshore oil and gas operations, focusing on the risks associated with various types of equipment, particularly on the risk of fire. It identifies specific machinery and systems that could pose hazards, assesses their potential impact on safety, and explores conditions that may lead to accidents. Some of the largest accidents were analyzed for their associations with fire hazards and specific equipment. Two primary regulatory approaches to offshore safety are examined: the prescriptive approach in the United States (US) and the goal-oriented approach in Europe. The prescriptive approach mandates strict compliance with specific regulations, while in the goal-oriented approach a failure to adhere to recognized best practices can result in legal accountability for negligence, especially concerning human life and environmental protection. This article also reviews achievements in safety through the efforts of regulatory authorities, industry collaborations, technical standards, and risk assessments, with particular attention given to the status of Mobile Offshore Drilling Units (MODUs). Contrary to common belief, the most frequent types of accidents are not those involving a fire/explosion caused by the failure of the Blowout Preventer (BOP) after a well problem has already started. Following analysis, it can be concluded that the most frequent type of accident typically occurs without fire and is due to material fatigue. This can result in the collapse of the facility, capsizing of the platform, and loss of buoyancy of mobile units, particularly in bad weather or during towing operations. It cannot be concluded that accidents can be more efficiently prevented under a specific type of safety regime, whether prescriptive or goal-oriented.
Unravelling the Digestibility and Structure–Function Relationship of Lentil Protein Through Germination and Molecular Weight Fractionation
Authors: Mirzapour-Kouhdasht Armin , Shaghaghian Samaneh , Majdinasab Marjan , Huang Jen-Yi , Garcia-Vaquero Marco
Published: January 2025

This study explores for the first time the impact of a 6-day germination process on the structure (FTIR), antioxidant activity, nutritional/safety attributes (ACE-I inhibitory activity, digestibility, and cytotoxicity), and functional properties of fractions of variable molecular weight (W > 5 kDa; 3 kDa < MW < 5 kDa; and MW < 3 kDa) isolated from proteins extracted from lentils. FTIR results indicated a substantial increase in β-sheet contents during germination. The digestibility of proteins increased from day 0 (16.32–17.04%) to day 6 of germination (24.92–26.05%) with variable levels of digestibility depending on their MW. ACE-I inhibitory activity improved during germination in all fractions, reaching IC50 values of 0.95, 0.83, and 0.69 mg/mL after 6 days of germination. All antioxidant activities analyzed notably increased, particularly in low-MW fractions (MW < 3 kDa). The functional properties of low-MW fractions were also the most promising, displaying the highest water and fat binding capacities and emulsifying and foaming capacities but lower foaming and emulsifying stability compared to high-MW fractions. Cytotoxicity tests on L929 cells revealed the slight adverse effects of low-MW fractions during germination. This study provides insights into the enhanced nutritional and functional attributes of lentil proteins following germination, emphasizing their potential application in functional foods.
Effects of Diets Containing Extruded Linseed and Padina pavonica Algae on Meat Rabbit: Carcass Performance and Meat Quality
Authors: Fehri Nour Elhouda , Contò Michela , Castrica Marta , Quattrone Alda , Renzi Gianluca , Di Giovanni Sabrina , Agradi Stella , Vigo Daniele , Brecchia Gabriele , Menchetti Laura , Balzaretti Claudia Maria , Beqiraj Doriana , Andoni Egon , Curone Giulio , Failla Sebastiana
Published: January 2025

This study investigated the effects of dietary supplementation with extruded linseed (ELS) and Padina pavonica algae extract (PP) on rabbit carcass and meat quality. Ninety-six rabbit carcasses from two production cycles were analyzed. In the first cycle (C1), rabbits were fed a control diet (1CNT), the same diet supplemented with 5% ELS (1ELS5%), and supplemented with 3.5% ELS and 0.2% PP (1LPP3.5%). In the second cycle (C2), the diets varied in composition and supplementation levels: a different control diet (2CNT), the same diet with 5% ELS (2ELS5%), and with 5% ELS and 0.2% PP (2LPP5%). Meat analyses were performed on Longissimus thoracis et lumborum (LTL) muscle for physical properties and on thigh meat (THM) for proximate composition, vitamin E, coenzyme-Q10, cholesterol, fatty acid profile, and mineral content. No significant differences in LTL physical quality were observed in C1, although LTL was brighter in C2 (p < 0.001). THM in C2 had higher fat content (p < 0.001). Dietary supplementation with ELS and PP extract significantly increased polyunsaturated fatty acids (n-3 PUFAs) and improved the n-6/n-3 ratio (p < 0.001) in rabbit meat, demonstrating their positive impact on meat quality.
Mare’s and Cow’s Milk Fortified with Flaxseed Oil Through Freeze-Drying Microencapsulation: Physicochemical and Nutritional Properties
Authors: Gawałek Jolanta , Cais-Sokolińska Dorota , Teichert Joanna
Published: January 2025

The microencapsulation via freeze drying of flaxseed oil in cow and mare milk was analyzed. The physicochemical and nutritional properties of the four obtained freeze-dried powder products were comparatively analyzed: microencapsulated and fortified with flaxseed oil cow milk (CMFO) and mare milk (MMFO), as well as pure cow milk (CM) and pure mare milk (MM). The moisture content, water activity, particle size distribution (PSD), loose and tapped bulk densities, flowability, color, and fatty acid profiles of the freeze-dried powders as well as the PSD of reconstituted emulsion droplets were investigated. For both types of milk, the fortified products achieved lower moisture content and water activity, higher loose and tapped bulk densities, better flowability, and lower particle sizes for the reconstituted emulsion droplets. The PSDs of the powders and reconstituted emulsion droplets showed significantly lower levels for the mare milk products than the cow milk products. The atherogenic index (AI) and thrombogenic index (TI) of cow milk products were three and six times higher than those for mare milk products, respectively. In both types of milk, fortified products did not show differences in their AI and TI values, except for the TI for cow milk (where CMFO < CM). The hypercholesterolemia fatty acid index (HcFA), hypocholesterolemic acids (DFAs), hypercholesterolemic acids content (OFA), and n-6/n-3 fatty acid ratio showed greater health benefits from mare milk products. Fortification with flaxseed oil showed increased health-promoting properties in the case of the DFA and OFA parameters (for mare milk) and n-6/n-3 ratio (for cow milk).
Assessment of Protein Quality and Nutritional Characteristics of Commonly Consumed Pulses in the Caribbean Diet by Different In Vitro Assays
Authors: Thomas Daniel J. , Lu Zhanhui , Brummer Yolanda , Zhu Yan , Liu Ronghua , Mats Lili , Tsao Rong , Ramdath D. Dan , Nosworthy Matthew G.
Published: January 2025

Pulses, the dried seeds of leguminous plants, form an important part of the diets of many cultures, including Caribbean cuisine, and are a rich source of protein, carbohydrates, and antioxidants while being low in fats. This study examined the effect of a traditional home-cooking method on the nutritional characteristics of pulses commonly consumed in the Caribbean: red kidney beans and cranberry beans (Phaseolus vulgaris L.), cowpeas (Vigna unguiculata L.), and pigeon peas (Cajanus cajan L.). Protein quality, determined via three in vitro protein digestibility methods, starch, and phenolic content were determined in pre- and post-cooked samples using established methods. Pulses contained 20–26% protein, and cooking improved protein digestibility on average by 14.0 ± 2.5% (p < 0.05). However, notable differences in digestibility were observed: it was higher in static assays (pH-Drop and pH-Stat) than in the two-step digestibility assay. Average protein digestibility-corrected amino acid score (IVPDCAAS) among cooked pulses was 0.81 ± 0.14, with the highest in cranberry bean (0.82) and cowpea (0.88). Cooking modified pulse starch profiles by increasing total digestible starch. However, resistant starch and slowly digestible starch fractions accounted for approximately 20–25% of total cooked starch content. While total phenolic content (TPC) and antioxidant activity were reduced with cooking, they were within expected ranges for cooked pulse flours; however, they were higher in bean (P. vulgaris) varieties than cowpea and pigeon pea. These findings support the promotion of increased pulse consumption in Caribbean diets. Home cooking is a simple method to enhance pulse protein quality through enhancing digestibility; however, in vitro protein digestibility assays may require further standardization.
Recent Advances of Processing and Detection Techniques on Crustacean Allergens: A Review
Authors: Qu Xin , Ma Zekun , Wu Xuli , Lv Liangtao
Published: January 2025

Crustaceans are delicious and highly nutritional food. However, crustaceans are one of the main food allergens, causing severe public health issues. Thus, it is important to increase the knowledge on crustacean allergens and protect the health of sensitized individuals. This review systematically summarizes the basic information on major crustacean allergens’ characteristics, structures, and function. It also summarizes the latest evaluation and detection methods of crustacean allergens. In addition, various processing techniques to alleviate crustacean’s allergenicity are discussed and compared. A host of multiplex approaches as innovative research is attractive to decrease crustacean allergenicity. In addition, the strategies to address the risk of crustacean allergens are also reviewed and discussed in detail. This review provides updates and new findings on crustacean allergens, which helps better understand crustacean allergy and provide novel strategies for its prevention and management.
Identification of Oligopeptides in the Distillates from Various Rounds of Soy Sauce-Flavored Baijiu and Their Effect on the Ester–Acid–Alcohol Profile in Baijiu
Authors: Wu Qiang , Tian Shanlin , Zhang Xu , Zhao Yunhao , Yu Yougui
Published: January 2025

Endogenous peptides in Baijiu have primarily focused on finished liquor research, with limited attention given to the peptides in base liquor prior to blending. Liquid chromatography–tandem mass spectrometry (LC-MS) was employed to identify endogenous peptides in the distillates from the first to seventh rounds of soy sauce-flavored Baijiu. Two hundred and five oligopeptides were identified from these distillates, all of which had molecular weights below 1000 Da and were composed of amino acid residues associated with flavor (sweet, sour, and bitter) and biological activity. Furthermore, full-wavelength scanning, content determination of the main compounds, and molecular docking were performed to analyze these oligopeptides’ effect on the ester–acid–alcohol profile in Baijiu. This determination revealed a negative correlation between the peptide content and total ester content (r = −0.691), as well as the total acid content (r = −0.323), and a highly significant negative correlation with ethanol content (r = −0.916). Notably, the screened peptides (TRH, YHY, RQTQ, PLDLTSFVLHEAI, KHVS, LPQRHRMVYSLL, and NEWH) had specific interactions with the major flavor substances via hydrogen bonds, including esters (ethyl acetate, ethyl butanoate, ethyl hexanoate, and ethyl lactate), acids (acetate acid, butanoate acid, hexanoate acid, lactate acid), and alcohols (ethanol, 1-propanol, 1-butanol, and 1-hexanol). These findings elucidate the distribution and dynamic changes of endogenous peptides in the distillates from various rounds of soy sauce-flavored Baijiu, providing a theoretical foundation for further investigation into their interaction mechanisms associated with flavor compounds.
Short- and Long-Term Effects on Physical Fitness in Older Adults: Results from an 8-Week Exercise Program Repeated in Two Consecutive Years
Authors: Godhe Manne , Nilsson Johnny , Andersson Eva A.
Published: January 2025

Introduction: Information on the long-term maintenance of short-term exercise fitness gains measured by field-based tests is scarce in older adults. This study aimed to investigate short- and long-term changes in various physical fitness parameters after an 8-week exercise program. Methods: In this longitudinal study, a total of 265 participants (62% women; mean age 71.4 ± 4.7 years) completed a field-based test battery of 12 fitness tests (22 parameters) at 2 pre-tests and 1 post-test following an 8-week exercise program (2 sessions/week, combining aerobic and strength activities) in 2 consecutive years. The tests assessed muscle endurance, muscle strength, cardiorespiratory fitness, and motor fitness. Results: Significant short-term improvements were observed, e.g., in isometric trunk flexion and extension endurance (21–37%) for both sexes in both years. Lower-body muscular endurance improved in the first year (9–12%) for both sexes, while cardiorespiratory fitness (6-min walk test) improved only for men in both years (3%). No changes were seen in submaximal cycle test heart rates or any balance tests in any year. Most fitness parameters did not significantly decrease during the 9-month inter-intervention period, with a few exceptions in trunk strength and walking distance. Conclusions: This study demonstrates physical fitness improvements in older adults following short-term exercise interventions and that some of these improvements were maintained long term, whereas a few of these physical fitness test improvements decreased significantly over 9 months in older adults.
The Influence of Prefabricated Foot Orthosis Use on the Modification of Foot Posture in Adults with Pronated Feet: A Randomised Controlled Trial
Authors: Cáceres-Madrid María Victoria , Calderón-García Julián Fernando , Rodríguez-Velasco Francisco José , Basilio-Fernández Belinda , López-Espuela Fidel , Santano-Mogena Esperanza , Fontán-Jiménez Marina , Rico-Martín Sergio
Published: January 2025

Background: The use of foot orthoses to treat different pathologies in pronated feet in adults is widespread among podiatric professionals, although it has not been conclusively demonstrated to modify foot posture in the short or medium term. Objective: The aim of this study was to evaluate whether prefabricated foot supports reduce pronated foot posture in adults, as measured by the foot posture index (FPI). Methods: A randomised controlled clinical trial was conducted in 109 subjects with pronated feet. The participants were randomly placed into a control group that did not receive any intervention and an experimental group that used prefabricated orthoses for 6 months. The changes in the FPI were evaluated in both groups at 6 months. Results: Over the six-month follow-up period, the delta FPI variable was changed by −1.1 ± 2.2 points in the experimental group, whereas the same variable was reduced by 1.2 ± 2.1 points in the control group (p = 0.001). The participants in the experimental group neutralised their FPIs significantly more than those in the control group did (39.3% vs. 8.5%; p = 0.041). Moreover, individuals in the experimental group were more likely to migrate from highly pronated feet to pronated feet than those in the control group were (45.8% vs. 20%; p < 0.001). Finally, multivariate analysis indicated that prefabricated foot orthoses were associated with an improved FPI (OR: 6.23, CI%95: 2.72–17.09; p < 0.001). However, the corrective effect provided by the prefabricated foot orthoses, which neutralised the pronated posture, was nullified by the presence of index minus metatarsal formula. Conclusions: The use of prefabricated orthoses resulted in a decreased FPI in adults, especially in those with highly pronated feet. However, the index minus presence nullified the effect of prefabricated orthoses on foot posture neutralisation.
MicroRNA-200c in Cancer Generation, Invasion, and Metastasis
Authors: Guo Honghao , Zhang Ning , Huang Tao , Shen Na
Published: January 2025

MicroRNA-200c (miR-200c) is increasingly recognized as a crucial small RNA molecule that plays a significant and multifaceted role in the complex processes of tumor development, invasion, and metastasis across various types of cancers. Recent studies have compellingly demonstrated that miR-200c exerts its influence on tumor biology by meticulously regulating a range of critical processes, including cell proliferation, apoptosis, epithelial–mesenchymal transition (EMT), and cell migration, all of which are essential for the progression and aggressiveness of tumors. This comprehensive review aims to summarize the expression characteristics and functional implications of miR-200c across a diverse array of tumor types, delving into its potential utility as both a biomarker for early detection and a therapeutic target in the realm of cancer treatment. By synthesizing current research findings and insights, we aspire to provide valuable information that could significantly enhance early diagnostic capabilities and inform the strategic development of targeted therapy approaches in oncology.
Epstein–Barr Virus (EBV) and Human Papilloma Virus (HPV) in Gastric Cancers, with Special Reference to Gastric Cancer at a Young Age—A Pilot Study in Poland
Authors: Mazurek Marek , Jaros Małgorzata , Gliwa Anna M. , Sitarz Monika Z. , Dudzińska Ewa , Zinkiewicz Krzysztof , Sitarz Robert
Published: January 2025

Gastric cancer (GC) is one of the most common cancers in the world. It is a multi-factorial disease influenced by both genetic and environmental factors such as diet, obesity, radiation exposure, and infectious agents. Viral infections usually lead to chronic inflammation, which can initiate the development of cancers. To date, only a few studies have been published about Epstein–Barr virus (EBV) and human papillomavirus (HPV) infections in the context of the development of GC. In particular, research on the development of cancer among people under 45 years of age, including the impacts of EBV and HPV, is rare, and clear results have not been obtained. The aim of this study was to analyze the frequency of occurrence of EBV and HPV in GC, particularly in early-onset gastric cancer (EOGC). Tissue material from 135 patients with GC, including 84 men and 51 women, was examined. RT-PCR was performed to detect EBV, and PCR was performed to detect HPV. There were no significant impacts of EBV and HPV infections on any subtype of GC. There was also no statistically significant dependence of gender and location of the tumor on any subtype of GC. Further research on the impacts of infectious agents such as EBV and HPV on GC should be conducted using larger populations.
Strategies for Survival of Staphylococcus aureus in Host Cells
Authors: Xu Huiling , Wang Shengnan , Liu Xiaoting , Li Muzi , Wang Xiaozhou , Chen Huahua , Qu Chaonan , Liu Yongxia , Liu Jianzhu
Published: January 2025

Staphylococcus aureus, a common pathogen, is capable of producing a significant array of toxins and can develop biofilms or small colony variants (SCVs) to evade detection by the immune system and resist the effects of antibiotics. Its ability to persist for extended periods within host cells has led to increased research interest. This review examines the process of internalization of S. aureus, highlighting the impact of its toxins and adhesion factors on host cells. It elucidates the intricate interactions between them and the host cellular environment, thereby offering potential strategies for the treatment and prevention of S. aureus infections.
Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function
Authors: Rogacka Dorota , Rachubik Patrycja , Typiak Marlena , Kulesza Tomasz , Audzeyenka Irena , Saleem Moin A. , Sikora Honorata , Gruba Natalia , Wysocka Magdalena , Lesner Adam , Piwkowska Agnieszka
Published: January 2025

Microalbuminuria is the earliest clinical abnormality in diabetic kidney disease. High glucose (HG) concentrations are associated with the induction of oxidative stress in podocytes, leading to disruption of the glomerular filtration barrier. Our recent study revealed a significant decrease in the membrane-bound fraction of Klotho in podocytes that were cultured under HG conditions. Given that disintegrin and metalloproteinase 17 (ADAM17) is responsible for the shedding of Klotho from the cell membrane, the present study investigated the impact of HG on the interplay between ADAM17 and Klotho in human podocytes. We demonstrated that ADAM17 protein levels significantly increased in urine, renal tissue, and glomeruli from diabetic rats, with a concomitant increase in glomerular albumin permeability. High glucose increased ADAM17 extracellular activity, NADPH oxidase activity, and albumin permeability in podocytes. These effects were reversed after treatment with ADAM17 inhibitor, in cells with downregulated ADAM17 expression, or after the addition of Klotho. Additionally, elevations of extracellular ADAM17 activity were observed in podocytes with the downregulation of Klotho expression. Our data indicate a novel mechanism whereby hyperglycemia deteriorates podocyte function via ADAM17 activation. We also demonstrated the ability of Klotho to protect podocyte function under hyperglycemic conditions in an ADAM17-dependent manner.
Nesfatin-1 Neurons in the Ventral Premammillary Nucleus Integrate Metabolic and Reproductive Signals in Male Rats
Authors: Papp Rege Sugárka , Könczöl Katalin , Sípos Klaudia , Tóth Zsuzsanna E.
Published: January 2025

The ability to reproduce depends on metabolic status. In rodents, the ventral premammillary nucleus (PMv) integrates metabolic and reproductive signals. While leptin (adiposity-related) signaling in the PMv is critical for female fertility, male reproductive functions are strongly influenced by glucose homeostasis. The anorexigenic peptide nesfatin-1 is a leptin-independent central regulator of blood glucose. Therefore, its integrative role in male rats can be assumed. To investigate this, we mapped the distribution of nesfatin-1 mRNA- and protein-producing cells in the PMv during postnatal development via in situ hybridization and immunohistochemistry, respectively. Fos-nesfatin-1, double immunostaining was used to determine the combined effect of heterosexual pheromone challenge and insulin-induced hypoglycemia on neuronal activation in adults. We found that ~75% of the pheromone-activated neurons were nesfatin-1 cells. Hypoglycemia reduced pheromone-induced cell activation, particularly in nesfatin-1 neurons. Immuno-electron microscopy revealed innervation of PMv nesfatin-1 neurons by urocortin3-immunoreactive terminals, reportedly originating from the medial amygdala. Nesfatin-1 immunopositive neurons expressed GPR10 mRNA, a receptor associated with metabolic signaling, but did not respond with accumulation of phosphorylated STAT3 immunopositivity, a marker of leptin receptor signaling, in response to intracerebroventricular leptin treatment. Our results suggest that PMv nesfatin-1 neurons are primarily responsible for integrating reproductive and metabolic signaling in male rats.
[18F]FDG PET-Based Radiomics and Machine Learning for the Assessment of Gliomas and Glioblastomas: A Systematic Review
Authors: Dondi Francesco , Gatta Roberto , Gazzilli Maria , Bellini Pietro , Viganò Gian Luca , Ferrari Cristina , Pisani Antonio Rosario , Rubini Giuseppe , Bertagna Francesco
Published: January 2025

Background: Some evidence of the value of 18F-fluorodesoxyglucose ([18F]FDG) positron emission tomography (PET) imaging for the assessment of gliomas and glioblastomas (GBMs) is emerging. The aim of this systematic review was to assess the role of [18F]FDG PET-based radiomics and machine learning (ML) in the evaluation of these neoplasms. Methods: A wide literature search of the PubMed/MEDLINE, Scopus, and Cochrane Library databases was made to find relevant published articles on the role of [18F]FDG PET-based radiomics and ML for the assessment of gliomas and GBMs. Results: Eight studies were included in the systematic review. Signatures, including radiomics analysis and ML, generally demonstrated a possible diagnostic value to assess different characteristics of gliomas and GBMs, such as the methylation status of the O6-methylguanine-DNA methyltransferase (MGMT) promoter, the isocitrate dehydrogenase (IDH) genotype, alpha thalassemia/mental retardation X-linked (ATRX) mutation status, proliferative activity, differential diagnosis with solitary brain metastases or primary central nervous system lymphoma, and prognosis of these patients. Conclusion: Despite some intrinsic limitations of radiomics and ML affecting the studies included in the review, some initial insights on the promising role of these technologies for the assessment of gliomas and GBMs are emerging. Validation of these preliminary findings in multicentric studies is needed to translate radiomics and ML approaches in the clinical setting.
Interindividual Variability Response to Resistance and High-Intensity Interval Training on Blood Pressure Reduction in Hypertensive Older Adults
Authors: Cano-Montoya Johnattan , Hurtado Nicolas , Núñez Vergara Carolina , Báez Vargas Sebastián , Rojas-Vargas Marcela , Martínez-Huenchullán Sergio , Alvarez Cristian , Izquierdo Mikel
Published: January 2025

Background: This study evaluated the effects of resistance training (RT) and high-intensity interval training (HIIT) on systolic (SBP) and diastolic blood pressure (DBP) in hypertensive older adults undergoing pharmacological therapy over four and eight weeks. We compared the efficacy of RT and HIIT in reducing non-responders (NRs) between weeks 4 and 8 and analyzed time-course adaptations in NRs and responders (Rs). Methods: Thirty-nine participants were randomized into RT-G (n = 13), HIIT-G (n = 13), or control (CG, n = 13) groups. RT utilized elastic bands, and HIIT involved cycle ergometers, with three weekly 30 min sessions for 8 weeks. SBP and DBP were measured before intervention and at weeks 4 and 8, respectively. Individual responses were classified as NRs or Rs using the Hopkins method (SDIR = √[SDExp2–SDCon2]). Time-course adaptations were evaluated. Results: Both the RT-G and HIIT-G reduced SBP at 8 weeks (RT-G: −13 mmHg; [ES: 1.12]; HIIT-G: −12 mmHg [ES: 0.8]; both p < 0.05). The proportion of NRs for SBP decreased from 46% to 38% in RT-G and 69% to 46% in HIIT-G. Rs showed a peak SBP reduction at 4 weeks (−14.7 and −25.5 mmHg), stabilizing by week 8 (−22.8 and −19.6 mmHg) in RT-G and HIIT-G, respectively. Conclusion: Eight weeks of RT and HIIT effectively reduced SBP and NR prevalence, with time-course adaptations favoring Rs.