bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrophysiological Remodeling: Cardiac T-Tubules and ß-Adrenoceptors
Authors: Wright Peter T. , Gorelik Julia , Harding Sian E.
Published: September 2021

Beta-adrenoceptors (βAR) are often viewed as archetypal G-protein coupled receptors. Over the past fifteen years, investigations in cardiovascular biology have provided remarkable insights into this receptor family. These studies have shifted pharmacological dogma, from one which centralized the receptor to a new focus on structural micro-domains such as caveolae and t-tubules. Important studies have examined, separately, the structural compartmentation of ion channels and βAR. Despite links being assumed, relatively few studies have specifically examined the direct link between structural remodeling and electrical remodeling with a focus on βAR. In this review, we will examine the nature of receptor and ion channel dysfunction on a substrate of cardiomyocyte microdomain remodeling, as well as the likely ramifications for cardiac electrophysiology. We will then discuss the advances in methodologies in this area with a specific focus on super-resolution microscopy, fluorescent imaging, and new approaches involving microdomain specific, polymer-based agonists. The advent of powerful computational modelling approaches has allowed the science to shift from purely empirical work, and may allow future investigations based on prediction. Issues such as the cross-reactivity of receptors and cellular heterogeneity will also be discussed. Finally, we will speculate as to the potential developments within this field over the next ten years.
Radiotherapy Combined with PD-1 Inhibition Increases NK Cell Cytotoxicity towards Nasopharyngeal Carcinoma Cells
Authors: Makowska Anna , Lelabi Nora , Nothbaum Christina , Shen Lian , Busson Pierre , Tran Tram Thi Bao , Eble Michael , Kontny Udo
Published: September 2021

Background: Nasopharyngeal carcinoma (NPC) in endemic regions and younger patients is characterized by a prominent lymphomononuclear infiltration. Radiation is the principal therapeutic modality for patients with NPC. Recent data suggest that the efficacy of radiotherapy in various cancers can be augmented when combined with immune checkpoint blockade. Here, we investigate the effect of radiotherapy on the killing of NPC cells by Natural Killer (NK) cells. Methods: NPC cell lines and a patient-derived xenograft were exposed to NK cells in the context of radiotherapy. Cytotoxicity was measured using the calcein-release assay. The contribution of the PD-L1/PD-1 checkpoint and signaling pathways to killing were analyzed using specific inhibitors. Results: Radiotherapy sensitized NPC cells to NK cell killing and upregulated expression of PD-1 ligand (PD-L1) in NPC cells and PD-1 receptor (PD-1) in NK cells. Blocking of the PD-L1/PD-1 checkpoint further increased the killing of NPC cells by NK cells in the context of radiotherapy. Conclusion: Radiation boosts the killing of NPC cells by NK cells. Killing can be further augmented by blockade of the PD-L1/PD-1 checkpoint. The combination of radiotherapy with PD-L1/PD-1 checkpoint blockade could therefore increase the efficacy of radiotherapy in NPC tumors.
Impact of ABC Transporters in Osteosarcoma and Ewing’s Sarcoma: Which Are Involved in Chemoresistance and Which Are Not?
Authors: Serra Massimo , Hattinger Claudia Maria , Pasello Michela , Casotti Chiara , Fantoni Leonardo , Riganti Chiara , Manara Maria Cristina
Published: September 2021

The ATP-binding cassette (ABC) transporter superfamily consists of several proteins with a wide repertoire of functions. Under physiological conditions, ABC transporters are involved in cellular trafficking of hormones, lipids, ions, xenobiotics, and several other molecules, including a broad spectrum of chemical substrates and chemotherapeutic drugs. In cancers, ABC transporters have been intensely studied over the past decades, mostly for their involvement in the multidrug resistance (MDR) phenotype. This review provides an overview of ABC transporters, both related and unrelated to MDR, which have been studied in osteosarcoma and Ewing’s sarcoma. Since different backbone drugs used in first-line or rescue chemotherapy for these two rare bone sarcomas are substrates of ABC transporters, this review particularly focused on studies that have provided findings that have been either translated to clinical practice or have indicated new candidate therapeutic targets; however, findings obtained from ABC transporters that were not directly involved in drug resistance were also discussed, in order to provide a more complete overview of the biological impacts of these molecules in osteosarcoma and Ewing’s sarcoma. Finally, therapeutic strategies and agents aimed to circumvent ABC-mediated chemoresistance were discussed to provide future perspectives about possible treatment improvements of these neoplasms.
Ferroptosis Meets Cell–Cell Contacts
Authors: Dietrich Cornelia , Hofmann Thomas G.
Published: September 2021

Ferroptosis is a regulated form of cell death characterized by iron dependency and increased lipid peroxidation. Initially assumed to be selectively induced in tumour cells, there is increasing evidence that ferroptosis plays an important role in pathophysiology and numerous cell types and tissues. Deregulated ferroptosis has been linked to human diseases, such as neurodegenerative diseases, cardiovascular disorders, and cancer. Along these lines, ferroptosis is a promising pathway to overcoming therapy resistance of cancer cells. It is therefore of utmost importance to understand the cellular signalling pathways and the molecular mechanisms underlying ferroptosis regulation, including context-specific effects mediated by the neighbouring cells through cell–cell contacts. Here, we give an overview on the molecular events and machinery linked to ferroptosis induction and commitment. We further summarize and discuss current knowledge about the role of cell–cell contacts, which differ in ferroptosis regulation between normal somatic cells and cancer cells. We present emerging concepts on the underlying mechanisms, address open questions, and discuss the possible impact of cell–cell contacts on exploiting ferroptosis in cancer therapy.
Optimization of Electronic Nose Sensor Array for Tea Aroma Detecting Based on Correlation Coefficient and Cluster Analysis
Authors: Wang Jin , Zhang Cheng , Chang Meizhuo , He Wei , Lu Xiaohui , Fei Shaomei , Lu Guodong
Published: September 2021

The electronic nose system is widely used in tea aroma detecting, and the sensor array plays a fundamental role for obtaining good results. Here, a sensor array optimization (SAO) method based on correlation coefficient and cluster analysis (CA) is proposed. First, correlation coefficient and distinguishing performance value (DPV) are calculated to eliminate redundant sensors. Then, the sensor independence is obtained through cluster analysis and the number of sensors is confirmed. Finally, the optimized sensor array is constructed. According to the results of the proposed method, sensor array for green tea (LG), fried green tea (LF) and baked green tea (LB) are constructed, and validation experiments are carried out. The classification accuracy using methods of linear discriminant analysis (LDA) based on the average value (LDA-ave) combined with nearest-neighbor classifier (NNC) can almost reach 94.44~100%. When the proposed method is used to discriminate between various grades of West Lake Longjing tea, LF can show comparable performance to that of the German PEN2 electronic nose. The electronic nose SAO method proposed in this paper can effectively eliminate redundant sensors and improve the quality of original tea aroma data. With fewer sensors, the optimized sensor array contributes to the miniaturization and cost reduction of the electronic nose system.
New Organic Materials Based on Multitask 2H-benzo[d]1,2,3-triazole Moiety
Authors: Torres-Moya Iván , Carrillo José Ramón , Díaz-Ortiz Ángel , Prieto Pilar
Published: September 2021

Multifunctionality is a desirable aspect in materials science. Indeed, the development of multifunctional compounds is crucial for sustainable chemistry by saving resources and time. In this sense, 2H-benzo[d]1,2,3-triazole (BTz) is an excellent candidate with promising characteristics, including its ability to self-assemble; its acceptor character, which enables the synthesis of donor-acceptor structures; and its facile modulation using standard chemical methods. Thus, due to its interesting properties, it is possible to produce different derivatives with applications in different fields, as summarized in this article, with the correct substitution at the BTz cores. Optoelectronic or biomedical applications, amongst others, are highlighted.
Young Children Feeding Practices: An Update from the Sultanate of Oman
Authors: Al Maamari Salima , Al Shammakhi Saleh , Alghamari Ibtisam , Jabbour Jana , Al-Jawaldeh Ayoub
Published: September 2021

Despite proven benefits, most countries fail to meet international targets for appropriate complementary and Breast Feeding (BF) practices. This study assessed feeding practices of children under two years of age and correlated them with family parameters in Oman, a high income country in the Eastern Mediterranean Region. Methods: Data from this study originated from the latest Oman National Nutrition Survey (ONNS). Assessment of children and their mothers’ socioeconomic, anthropometric, and nutritional variables was conducted at the household level. Evaluated feeding practices included age appropriate BF, diet diversity, and minimum acceptable diet (MAD). Results: Pairs of mothers and infants (n = 1344) were assessed. Early BF, exclusive BF at 6 months, infant formula, and iron rich meals were provided to 81, 29, 44, and 84% of children, respectively. Age appropriate BF and MAD were found in 58% and 35% of children, respectively. Low maternal education, younger age, low household income, and governorate negatively affected diet acceptability. Conclusion: Omani children successfully received early BF postpartum and consumed iron rich meals. Yet, rates of exclusive BF rates at six months and MAD for children under two were low. Comprehensive strategies should be placed to assess and influence children feeding practices in the Sultanate.
A Case Series on Genotype and Outcome of Liver Transplantation in Children with Niemann-Pick Disease Type C
Authors: Modin Line , Ng Vicky , Gissen Paul , Raiman Julian , Pfister Eva Doreen , Das Anibh , Santer René , Faghfoury Hanna , Santra Saikat , Baumann Ulrich
Published: September 2021

Background: To report on clinical presentation and outcomes of children who underwent liver transplantation (LTx) and were subsequently diagnosed to have Niemann-Pick type C (NPC). Methods: Retrospective, descriptive, multi-centre review of children diagnosed with NPC who underwent LTx (2003–2018). Diagnosis was made by filipin skin test or genetic testing. Results: Nine children were identified (six centres). Neonatal acute liver failure was the most common indication for LTx (seven children). Median age at first presentation: 7 days (range: 0–37). The most prevalent presenting symptoms: jaundice (8/9), hepatosplenomegaly (8/9) and ascites (6/9). 8/9 children had a LTx before the diagnosis of NPC. Genetic testing revealed mutations in NPC1 correlating with a severe biochemical phenotype in 5 patients. All 9 children survived beyond early infancy. Seven children are still alive (median follow-up time of 9 (range: 6–13) years). Neurological symptoms developed in 4/7 (57%) patients at median 9 (range: 5–13) years following LTx. Conclusion: Early diagnosis of NPC continues to be a challenge and a definitive diagnosis is often made only after LTx. Neurological disease is not prevented in the majority of patients. Genotype does not appear to predict neurological outcome after LTx. LTx still remains controversial in NPC.
Distributed Energy Balance Flux Modelling of Mass Balances in the Artesonraju Glacier and Discharge in the Basin of Artesoncocha, Cordillera Blanca, Peru
Authors: Lozano Gacha María Fernanda , Koch Manfred
Published: September 2021

A distributed energy balance model (DEBAM) is applied to estimate the mass balance of the Artesonraju glacier in the Cordillera Blanca (CB), Peru, and to simulate the ensuing discharge into its respective basin, Artesoncocha. The energy balance model calibrations show that, by using seasonal albedos, reasonable results for mass balances and discharge can be obtained, as witnessed by annually aggregated Nash Sutcliffe coefficients (E) of 0.60–0.87 for discharge and of 0.58–0.71 for mass measurements carried out in the period 2004–2007. Mass losses between −1.42 and −0.45 m.w.e. are calculated for that period. The elevation line altitudes (ELAs), which lie between 5009 and 5050 m.a.s.l., are also well simulated, compared to those measured by the Unidad Glaciologica de Recursos Hídricos del Perú (UGRH). It is demonstrated that the net radiation which drives the energy balance and melting processes is mainly affected by the amount of reflected shortwave radiation from the different surfaces. Moreover, the longwave radiation sinks between 63 and 73% of solar radiative energy in the dry season. Further sensitivity studies indicate that the assumed threshold temperature T0 is crucial in mass balance simulations, as it determines the extension of areas with different albedos. An optimal T0 between 2.6 and 3.8 °C is deduced from these simulations.
Dilated Cardiomyopathy Associated with Paraquat Herbicide Poisoning
Authors: Ahmad Jaffer , D’Angelo Kyla , Rivas Madalyn , Mahal Manpreet , Nookala Vikas , Kulakauskiene Dovile , Makaryus Amgad N.
Published: September 2021

Dilated cardiomyopathy is a subset of cardiomyopathies defined by reduced ejection fraction of less than 45% and a dilated left ventricle. While dilated cardiomyopathy is common, its etiology is not always readily evident. Paraquat is used as an herbicide worldwide and is one of the main causes of fatal poisoning in underdeveloped countries in Asia, Central America, and the Pacific Islands. The most commonly affected organs are the lungs and kidneys. However, experimental research has shown that Paraquat can affect the heart indirectly through increased vascular permeability. In vivo animal studies have shown that paraquat poisoning causes myocardial contractile dysfunction by decreased fractional shortening and cardiac remodeling. We report the first case in published literature of a 52-year-old Hispanic man with dilated cardiomyopathy strongly associated with Paraquat exposure. It is important to obtain detailed medical history and proper diagnostic work-up including work, social, and family history, and echocardiography, baseline EKG, lab work, and ischemia cardiac testing as it can lead to improved diagnostic evaluation of possible etiologies of the commonly seen dilated cardiomyopathies and help identify less well-known etiologies as seen in our patient.
The Influence of CBD Parameters on the Energy Gap of ZnS Narcissus-Like Nanostructured Thin Films
Authors: Khalil Mohammed Hussein , Mohammed Raghad Y. , Ibrahem Mohammed Aziz
Published: September 2021

Recently, the efficient preparation techniques of zinc sulfide (ZnS) nanostructured films have drawn great attention due to their potential applications in optoelectronics. In this study, the low-cost and high-yield chemical bath deposition (CBD) technique was used to deposit ZnS nanostructured thin films. The effect of various deposition parameters such as time, pH, precursor concentration, and temperature on the morphology and energy bandgap (Eg) of the prepared thin films were investigated. The characterization of the prepared thin films revealed the formation of polycrystalline ZnS with Narcissus-like nanostructures. Moreover, the optical characterization showed inverse proportionality between both the transmission and Eg of the nanostructured thin films and the variation of the deposition parameters. A range of different Eg values between 3.92 eV with 20% transmission and 4.06 eV with 80% transmission was obtained. Tuning the Eg values and transmission of the prepared nanostructured films by manipulating the deposition parameters of such an efficient technique could lead to applications in optoelectronics such as solar cells and detectors.
Effect of Nitrogen Flow Ratio on Degradation Behaviors and Failure of Magnetron Sputter Deposited Tantalum Nitride
Authors: Li Zhigang , Zhang Yubao , Wang Yi , Li Jinfeng , Zhao Hongtao
Published: September 2021

A series of Tantalum Nitride (TaN) films under a reactive direct current magnetron sputtering method with a controlled total gas flow rate were prepared on aluminum oxide substrates. To find the nitrogen flow rate, which produced the minimum sheet resistance, TaN films deposited under a nitrogen gas flow ratio of 2.5%, 5%, 10%, 15%, 20%, 25% were characterized in terms of their structural and electrical properties. The optimum total gas flow rate was 60 sccm, revealing the lowest deviation of sheet resistance. Next, the durability and reliability at high temperatures, after heating and cooling cycles and exposure to the induced current, were tested. When the nitrogen flow ratio reaches 2.5%, it gets the maximum for the adhesion force, roughness, and deposition rate of the TaN film, and maximum values are 75.4 N, 1.1 nm, and 3.67 nm/min, respectively, and the sheet resistance of the TaN film reaches a minimum of 20.32 Ω/sq. The degradation behaviors and failure of TaN films were investigated by measuring the sheet resistance variation. To further explain the degradation of TaN films, additional analysis of their crystallinity was conducted. The results showed that TaN-based thin film resistors have high durability and reliability, and are suitable for embedded passive resistors.
Improved Corrosion Protection of Acrylic Waterborne Coating by Doping with Microencapsulated Corrosion Inhibitors
Authors: Ress Jacob , Martin Ulises , Bastidas David M.
Published: September 2021

Herein, a waterborne acrylic coating doped with pH sensitive colophony microcapsules containing corrosion inhibitors was studied on carbon steel plates. The changes in the physical properties of the coatings were studied. The microcapsule coating specimens maintained more noble Ecorr values compared to the control in deionized water and simulated concrete pore solutions with −513 and −531 mVSCE, respectively. Additionally, the microcapsule polarization results for both pH 12.6 and 6.2 electrolyte solutions showed lower icorr values of 1.20 × 10−6 and 3.24 × 10−6 A·cm−2, respectively, compared to the control sample (1.15 × 10−5 and 4.21 × 10−5 A·cm−2). Therefore, the microcapsule coating provided more protection from chloride attack on the substrate as well as the deleterious effects of low pH on carbon steel. The electrochemical impedance spectroscopy analysis corroborated the DC polarization results, showing increased corrosion resistance for the microcapsule coated specimens compared to the control. Moreover, the Rpore and Rct are much higher than the control, indicating the protection of the inhibitors. The Ceff,dl also shows lower values for the microcapsule coating than the control, showing a more protective and less doped double layer.
Near-Infrared Spectroscopy Study of Serpentine Minerals and Assignment of the OH Group
Authors: Wu Shaokun , He Mingyue , Yang Mei , Zhang Biyao , Wang Feng , Li Qianzhi
Published: September 2021

Three different kinds of serpentine mineral samples were investigated using Fourier transform near-infrared spectroscopy (FTNIR). The results show that there are obvious differences in the characteristic infrared spectra of the three serpentine group minerals (lizardite, chrysotile, and antigorite), which can easily be used to identify these serpentine minerals. The combination of weak and strong peaks in the spectrum of lizardite appears at 3650 and 3690 cm−1, while the intensities of the peaks at 4281 and 4301 cm−1 (at 7233 and 7241 cm−1, respectively) are similar. A combination of weak and strong peaks in chrysotile appears at 3648 and 3689 cm−1 and at 4279 and 4302 cm−1, and a single strong peak appears at 7233 cm−1. In antigorite, there are strong single peaks at 3674, 4301, and 7231 cm−1, and the remaining peaks are shoulder peaks or are not obvious. The structural OH mainly appears as characteristic peaks in four regions, 500–720, 3600–3750, 4000–4600, and 7000–7600 cm−1, corresponding to the OH bending vibration, the OH stretching vibration, the OH secondary combination vibration, and the OH overtone vibration, respectively. In the combined frequency vibration region, the characteristic peak near 4300 cm−1 is formed by the combination of the internal and external stretching vibrations and bending vibrations of the structural OH group. The overtone vibrations of the OH stretching vibration appear near 7200 cm−1, and the practical factor is about 1.965. The near-infrared spectra of serpentine minerals are closely related to their structural differences and isomorphous substitutions. Therefore, near-infrared spectroscopy can be used to identify serpentine species and provides a basis for studies on the genesis and metallogenic environment of these minerals.
Genetic Divergence between Two Sympatric Ecotypes of Phalaenopsis pulcherrima on Hainan Island
Authors: Hu Xiangyu , Lan Siren , Song Xiqiang , Yang Fusun , Zhang Zhe , Peng Donghui , Ren Mingxun
Published: September 2021

Ecotypes are the result of ecological differentiation at the early stages of speciation. Adaptation to soil conditions offers arguably the best examples of local adaptation in plants. Two sympatric ecotypes, with either a red or green abaxial leaf surface, were found without clear geographical isolation in Phalaenopsis pulcherrima, a Southeast Asia endemic and endangered orchid. The soil of the red leaf ecotype has a higher water content and nutrient content than the green ecotype. What is the genetic structure of the two ecotypes? Is there complete or partial reproductive isolation between the two ecotypes? In this work, leaf reflection of the two ecotypes in P. pulcherrima were compared, to illustrate their difference in leaf color. The genetic differentiation between two ecotypes was examined, using ISSR and SRAP markers to determine the genetic structure of the populations. Our results showed that the green ecotype had reflectance spectrum peaks at 530 nm and 620 nm, while in the red ecotype, the peak at 530 nm was absent. A total of 165 ISSR and SRAP loci showed a high level of genetic diversity within the green ecotype, and analyses of the population structure revealed two genetic clusters that corresponded to the red and green ecotypes. The percentage of variation between the two ecotypes (24.55%) was greater than the percentage of variation among the populations (16.54%)—indicating partial reproductive isolation, high genetic differentiation, and that ecological differentiation has been more important than geographical barriers among populations within ecotypes. Most pairwise FST values between the populations within either ecotype on Hainan Island were less than 0.15; however, the FST between both the Thai and Malaysian populations and the Hainan Island population was greater than 0.25, due to South China sea isolation. Ecotypic differentiation is an important part of speciation; therefore, we must take into account the axes along which lineages sort, when formulating protection strategies.
Dataset of Flow-Induced Vibrations on a Pipe Conveying Cold Water
Authors: Villa Francisco , Sánchez Cherlly , Vallejo Marcela , Botero-Valencia Juan S. , Delgado-Trejos Edilson
Published: September 2021

Analysis of flow-induced pipe vibrations has been applied in a variety of applications, such as flowrate inference and leak detection. These applications are based on a functional relationship between the vibration features estimated in the pipe walls and the dynamics related to the flow of the substance. The dataset described in this document is comprised of signals acquired using an accelerometer attached to a pipe conveying cold water at specific flowrate values. Tests were carried out under numerals of the ISO 4064-1/2: 2016 standard and were performed in two measurement benches designed for flowmeter calibration, and a total of 80 flowrate values, from 25 L/h to 20,000 L/h, were considered. For each flowrate value, 3 to 6 samples were taken, so that the resulting dataset has a total of 382 signals that contain acceleration values in three axes and a timestamp in microseconds.
Beat-to-Beat P-Wave Analysis Outperforms Conventional P-Wave Indices in Identifying Patients with a History of Paroxysmal Atrial Fibrillation during Sinus Rhythm
Authors: Tachmatzidis Dimitrios , Filos Dimitrios , Chouvarda Ioanna , Tsarouchas Anastasios , Mouselimis Dimitrios , Bakogiannis Constantinos , Lazaridis Charalampos , Triantafyllou Konstantinos , Antoniadis Antonios P. , Fragakis Nikolaos , Efthimiadis Georgios , Maglaveras Nicos , Tsalikakis Dimitrios G. , Vassilikos Vassilios P.
Published: September 2021

Early identification of patients at risk for paroxysmal atrial fibrillation (PAF) is essential to attain optimal treatment and a favorable prognosis. We compared the performance of a beat-to-beat (B2B) P-wave analysis with that of standard P-wave indices (SPWIs) in identifying patients prone to PAF. To this end, 12-lead ECG and 10 min vectorcardiogram (VCG) recordings were obtained from 33 consecutive, antiarrhythmic therapy naïve patients, with a short history of low burden PAF, and from 56 age- and sex-matched individuals with no AF history. For both groups, SPWIs were calculated, while the VCG recordings were analyzed on a B2B basis, and the P-waves were classified to a primary or secondary morphology. Wavelet transform was used to further analyze P-wave signals of main morphology. Univariate analysis revealed that none of the SPWIs performed acceptably in PAF detection, while five B2B features reached an AUC above 0.7. Moreover, multivariate logistic regression analysis was used to develop two classifiers—one based on B2B analysis derived features and one using only SPWIs. The B2B classifier was found to be superior to SPWIs classifier; B2B AUC: 0.849 (0.754–0.917) vs. SPWIs AUC: 0.721 (0.613–0.813), p value: 0.041. Therefore, in the studied population, the proposed B2B P-wave analysis outperforms SPWIs in detecting patients with PAF while in sinus rhythm. This can be used in further clinical trials regarding the prognosis of such patients.
Real-World Analysis of the EGFR Mutation Test in Tissue and Plasma Samples from Non-Small Cell Lung Cancer
Authors: Lee Hyunwoo , Han Joungho , Choi Yoon-La
Published: September 2021

Molecular evaluation of EGFR mutation is indispensable in treating non-small cell lung cancer (NSCLC). We compared the results of EGFR analysis using tissue DNA (tDNA) and circulating tumor (ctDNA) to evaluate the feasibility of plasma as an effective material for detecting EGFR mutation and the reliability of ctDNA analysis in real-world practice settings. We enrolled 554 NSCLC cases who had undergone ctDNA EGFR analysis between January 2019 and March 2020. EGFR mutations were detected in 240 (57.3%) of the 421 cases with EGFR mutations confirmed by tDNA analysis. In multivariate analysis, the size of the largest tumor deposits, disease progression, M stage, the detectable amount of tumor tissue with EGFR mutation in distant metastasis, liver metastasis, pleural seeding, and bone metastasis (p < 0.05) were identified as independent factors affecting the detection rate of EGFR mutations in ctDNA. Survival analysis revealed ctDNA status and M stage (p < 0.001) to be independent predictors of overall survival in the multivariate analysis. Our study demonstrates that EGFR analysis using ctDNA is a useful clinical tool and can aid in therapeutic decisions in real-world practical settings. However, clinicians should be aware of the possibility of false negatives and confirm EGFR analysis using tDNA in certain situations.
Investigation of Therapeutic Response Markers for Acupuncture in Parkinson’s Disease: An Exploratory Pilot Study
Authors: Park Sang-Min , Kim Aeyung , Park Gunhyuk , Kwon Ojin , Park Sangsoo , Yoo Horyong , Jang Jung-Hee
Published: September 2021

In this preliminary pilot study, we investigated the specific genes implicated in the therapeutic response to acupuncture in patients with Parkinson’s disease (PD). Transcriptome alterations following acupuncture in blood samples collected during our previous clinical trial were analyzed along with the clinical data of six patients with PD, of which a representative patient was selected for transcriptomic analysis following acupuncture. We also examined the changes in the expression of PD biomarker genes known to be dysregulated in both the brain and blood of patients with PD. We validated these gene expression changes using quantitative real-time polymerase chain reaction (qPCR) in the blood of the remaining five patients with PD who received acupuncture treatment. Following acupuncture treatment, the transcriptomic alterations in the representative patient were similar to those induced by dopaminergic therapy. Among the PD biomarkers, ankyrin repeat domain 22 (ANKRD22), upregulated following dopaminergic therapy, and synapsin 1 (SYN1), a common gene marker for synaptic dysfunction in PD, were upregulated following acupuncture. These alterations correlated with changes in gait parameters in patients with PD. Our data suggest ANKRD22 and SYN1 as potential biomarkers to predict/monitor therapeutic responses to acupuncture in patients with PD, especially in those with gait disturbance. Further research is needed to confirm these findings in a large sample of patients with PD.
The Significance of VDR Genetic Polymorphisms in the Etiology of Preeclampsia in Pregnant Polish Women
Authors: Magiełda-Stola Justyna , Kurzawińska Grażyna , Ożarowski Marcin , Karpiński Tomasz M. , Drews Krzysztof , Seremak-Mrozikiewicz Agnieszka
Published: September 2021

For the first time in the Polish population, we aimed to investigate associations between the VDR gene single-nucleotide polymorphisms (SNPs) BsmI (rs15444410), ApaI (rs7975232), FokI (rs19735810), and TaqI (rs731236) and the development of preeclampsia (PE). A case–control study surveyed 122 preeclamptic and 184 normotensive pregnant women. The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was performed to examine the maternal VDR FokI, BsmI, TaqI, and ApaI polymorphisms. The VDR BsmIAA homozygous genotype was statistically significantly more frequent in preeclamptic women compared to the control group (p = 0.0263), which was also associated with a 2-fold increased risk of PE (OR = 2.06, p = 0.012). A correlation between the VDR BsmI polymorphism with systolic and diastolic blood hypertension was noted. Furthermore, 3-marker haplotype CTA (TaqI/ApaI/BsmI) was associated with significantly higher systolic (p = 0.0075) and diastolic (p = 0.0072) blood pressure. Association and haplotype analysis indicated that the VDR BsmI A allele could play a significant role in the PE pathomechanism and hence could be a risk factor for PE development in pregnant Polish women. These results indicate the importance of the VDR BsmI polymorphism and reveal that this variant is closely associated with a higher predisposition to hypertension.
Ciliary Videomicroscopy: A Long Beat from the European Respiratory Society Guidelines to the Recognition as a Confirmatory Test for Primary Ciliary Dyskinesia
Authors: Bricmont Noemie , Alexandru Mihaela , Louis Bruno , Papon Jean-François , Kempeneers Céline
Published: September 2021

Primary ciliary dyskinesia (PCD) is a rare inherited ciliopathy in which respiratory cilia are stationary or dyskinetic. The clinical presentation of PCD is highly non-specific since it includes infections and disorders of the upper (otitis and rhinosinusitis) and lower (neonatal respiratory distress, bronchitis, pneumonia and bronchiectasis) airways, starting in early life. Clinical examination alone does not allow a PCD diagnosis, which relies on several concordant tests, since none are sensitive or specific enough alone. Despite being the most sensitive and specific test to diagnose PCD, digital high-speed videomicroscopy (DHSV) is not sufficiently standardized, preventing its use with complete confidence as a confirmatory diagnostic test for PCD, or its inclusion in a diagnostic algorithm. Since the 2017 ERS recommendations for PCD diagnosis, three main issues remain to be solved in order to optimize DHSV ciliary beating evaluation: the problem in defining an accurate sensitivity and specificity as there is no gold standard method to diagnose all PCD cases, a lack of standardization in the operating procedure for processing respiratory samples, and in the choice of measured parameters (self-operating or not). The development of new automated analysis approaches is promising and will require full clinical validation.
Comparison of Next-Generation Sequencing and Fluorescence In Situ Hybridization for Detection of Segmental Chromosomal Aberrations in Neuroblastoma
Authors: Kim Eojin , Lee Boram , Lee Ji Won , Sung Ki Woong , Kim Jung-Sun
Published: September 2021

The aim of this study was to compare next-generation sequencing (NGS) with the traditional fluorescence in situ hybridization (FISH) for detecting segmental chromosomal aberrations (SCAs) such as 1p deletion, 11q deletion and 17q gain, which are well-known predictive markers for adverse outcome in neuroblastoma. The tumor tissue obtained from 35 patients with neuroblastoma was tested by FISH and targeted NGS, which is specially designed to detect copy number alterations across the entire chromosomal region in addition to mutations in 353 cancer-related genes. All chromosomal copy number alterations were analyzed using the copy number variation plot derived from targeted NGS. FISH was performed to detect 1p deletion, 11q deletion and 17q gain. The copy numbers of 1p, 11q, and 17q obtained via NGS were correlated with those acquired via FISH. The SCAs determined by NGS were matched with those by FISH. Most 17q gain of mismatched cases detected by NGS alone showed a subsegmental gain of 17q. FISH revealed 11q deletion and 17q gain in a few tumor cells of two cases, which were not detected by NGS. NGS can be a sensitive complementary and alternative method to the conventional FISH for detecting SCAs.
High Prevalence of Pre-Existing Liver Abnormalities Identified Via Autopsies in COVID-19: Identification of a New Silent Risk Factor?
Authors: Hirayama Yuri , Daniels Natasha Faye , Evans Shelley , Clarke David , Purvis Stephenie , Oliver Charlotte , Woodmansey Stephen , Staniforth Joy , Soilleux Elizabeth J.
Published: September 2021

A high prevalence of hepatic pathology (in 17 of 19 cases) was reported in post-mortem (PM) examinations of COVID-19 patients, undertaken between March 2020 and February 2021 by a single autopsy pathologist in two English Coronial jurisdictions. The patients in our cohort demonstrated high levels of recognised COVID-19 risk factors, including hypertension (8/16, 50%), type 2 diabetes mellitus (8/16, 50%) and evidence of arteriopathy 6/16 (38%). Hepatic abnormalities included steatosis (12/19; 63%), moderate to severe venous congestion (5/19; 26%) and cirrhosis (4/19; 21%). A subsequent literature review indicated a significantly increased prevalence of steatosis (49%), venous congestion (34%) and cirrhosis (9.3%) in COVID-19 PM cases, compared with a pre-pandemic PM cohort (33%, 16%, and 2.6%, respectively), likely reflecting an increased mortality risk in SARS-CoV-2 infection for patients with pre-existing liver disease. To corroborate this observation, we retrospectively analysed the admission liver function test (LFT) results of 276 consecutive, anonymised COVID-19 hospital patients in our centre, for whom outcome data were available. Of these patients, 236 (85.5%) had significantly reduced albumin levels at the time of admission to hospital, which was likely indicative of pre-existing chronic liver or renal disease. There was a strong correlation between patient outcome (length of hospital admission or death) and abnormal albumin at the time of hospital admission (p = 0.000012). We discuss potential mechanisms by which our observations of hepatic dysfunction are linked to a risk of COVID-19 mortality, speculating on the importance of recently identified anti-interferon antibodies.
Neuropathic Pain and Ultrasonography: A Multiperspective Literature Evaluation
Authors: Coraci Daniele , Capobianco Serena , Romano Marcello , Calvaruso Salvatore , Vecchio Michele , Giovannini Silvia , Loreti Claudia , Fusco Augusto , Masiero Stefano , Santilli Valter , Padua Luca
Published: September 2021

Among the tools useful for the management of neuropathic pain, ultrasound presents several advantages, shown by the literature. We assessed the scientific production about neuropathic pain and ultrasound from different points of view: general topics, journal categories, geographical origin and lexical analysis. We searched papers on PubMed using the Medical Subject Headings “neuropathic pain” AND “ultrasound”. We collected data about the journals where the papers were published, the country of the affiliation of the first author. For the lexical analysis, we evaluated the presence of selected words in the papers, and we built a graph representing the connections among words and papers. The papers were focused on the use of ultrasound as a diagnostic tool and guide for the therapy, assessing its application in different diseases such as Morton’s neuroma and piriformis syndrome. The most represented journal category was anesthesia while the most common country the United States of America. The lexical analysis confirmed the importance of ultrasound for diagnosis of specific disease and treatment of pain. The described approaches provide a multiperspective evaluation of the literature and may support the interpretation of the information contained by the papers.
Circulating Hsa-miR-431-5p as Potential Biomarker for Squamous Cell Vulvar Carcinoma and Its Premalignant Lesions
Authors: Bujko Mateusz , Zalewski Kamil , Szczyrek Martyna , Kowalik Artur , Boresowicz Joanna , Długosz Angelika , Goryca Krzysztof , Góźdź Stanisław , Kowalewska Magdalena
Published: September 2021

Vulvar squamous cell carcinoma (VSCC) develops from high-grade squamous intraepithelial lesions (HSIL) and differentiated vulvar intraepithelial neoplasia (dVIN). This study aimed to assess the diagnostic value of circulating hsa-miR-431-5p in vulvar precancers and VSCC. Expression levels of hsa-miR-431-5p were analyzed by quantitative RT-PCR in plasma samples of 29 patients with vulvar precancers (HSIL or dVIN), 107 with VSCC as well as 15 healthy blood donors. We used hsa-miR-93-5p and hsa-miR-425-5p as normalizers. The levels of miR-431-5p were increased in the blood of patients with VSCC compared to those with vulvar precancers. Statistically significant differences in the survival rates (time to progression) were revealed for VSCC patients categorized by miR-431-5p levels. Low levels of circulating miR-431-5p were found to be indicative of unfavorable survival rates. In summary, our data reveal the diagnostic potential of circulating miR-431-5p in patients with vulvar precancers and VSCC.
Stochastic Chaos and Markov Blankets
Authors: Friston Karl , Heins Conor , Ueltzhöffer Kai , Da Costa Lancelot , Parr Thomas
Published: September 2021

In this treatment of random dynamical systems, we consider the existence—and identification—of conditional independencies at nonequilibrium steady-state. These independencies underwrite a particular partition of states, in which internal states are statistically secluded from external states by blanket states. The existence of such partitions has interesting implications for the information geometry of internal states. In brief, this geometry can be read as a physics of sentience, where internal states look as if they are inferring external states. However, the existence of such partitions—and the functional form of the underlying densities—have yet to be established. Here, using the Lorenz system as the basis of stochastic chaos, we leverage the Helmholtz decomposition—and polynomial expansions—to parameterise the steady-state density in terms of surprisal or self-information. We then show how Markov blankets can be identified—using the accompanying Hessian—to characterise the coupling between internal and external states in terms of a generalised synchrony or synchronisation of chaos. We conclude by suggesting that this kind of synchronisation may provide a mathematical basis for an elemental form of (autonomous or active) sentience in biology.
A Three-Dimensional Infinite Collapse Map with Image Encryption
Authors: Yan Wenhao , Jiang Zijing , Huang Xin , Ding Qun
Published: September 2021

Chaos is considered as a natural candidate for encryption systems owing to its sensitivity to initial values and unpredictability of its orbit. However, some encryption schemes based on low-dimensional chaotic systems exhibit various security defects due to their relatively simple dynamic characteristics. In order to enhance the dynamic behaviors of chaotic maps, a novel 3D infinite collapse map (3D-ICM) is proposed, and the performance of the chaotic system is analyzed from three aspects: a phase diagram, the Lyapunov exponent, and Sample Entropy. The results show that the chaotic system has complex chaotic behavior and high complexity. Furthermore, an image encryption scheme based on 3D-ICM is presented, whose security analysis indicates that the proposed image encryption scheme can resist violent attacks, correlation analysis, and differential attacks, so it has a higher security level.
A Novel Conflict Management Method Based on Uncertainty of Evidence and Reinforcement Learning for Multi-Sensor Information Fusion
Authors: Huang Fanghui , Zhang Yu , Wang Ziqing , Deng Xinyang
Published: September 2021

Dempster–Shafer theory (DST), which is widely used in information fusion, can process uncertain information without prior information; however, when the evidence to combine is highly conflicting, it may lead to counter-intuitive results. Moreover, the existing methods are not strong enough to process real-time and online conflicting evidence. In order to solve the above problems, a novel information fusion method is proposed in this paper. The proposed method combines the uncertainty of evidence and reinforcement learning (RL). Specifically, we consider two uncertainty degrees: the uncertainty of the original basic probability assignment (BPA) and the uncertainty of its negation. Then, Deng entropy is used to measure the uncertainty of BPAs. Two uncertainty degrees are considered as the condition of measuring information quality. Then, the adaptive conflict processing is performed by RL and the combination two uncertainty degrees. The next step is to compute Dempster’s combination rule (DCR) to achieve multi-sensor information fusion. Finally, a decision scheme based on correlation coefficient is used to make the decision. The proposed method not only realizes adaptive conflict evidence management, but also improves the accuracy of multi-sensor information fusion and reduces information loss. Numerical examples verify the effectiveness of the proposed method.
Continuous-Variable Quantum Secret Sharing Based on Thermal Terahertz Sources in Inter-Satellite Wireless Links
Authors: Liu Chengji , Zhu Changhua , Li Zhihui , Nie Min , Yang Hong , Pei Changxing
Published: September 2021

We propose a continuous-variable quantum secret sharing (CVQSS) scheme based on thermal terahertz (THz) sources in inter-satellite wireless links (THz-CVQSS). In this scheme, firstly, each player locally preforms Gaussian modulation to prepare a thermal THz state, and then couples it into a circulating spatiotemporal mode using a highly asymmetric beam splitter. At the end, the dealer measures the quadrature components of the received spatiotemporal mode through performing the heterodyne detection to share secure keys with all the players of a group. This design enables that the key can be recovered only by the whole group players’ knowledge in cooperation and neither a single player nor any subset of the players in the group can recover the key correctly. We analyze both the security and the performance of THz-CVQSS in inter-satellite links. Results show that a long-distance inter-satellite THz-CVQSS scheme with multiple players is feasible. This work will provide an effective way for building an inter-satellite quantum communication network.
Influence of Source Parameters on the Polarization Properties of Beams for Practical Free-Space Quantum Key Distribution
Authors: Wu Tianyi , Pan Qing , Lin Chushan , Shi Lei , Zhao Shanghong , Zhang Yijun , Wang Xingyu , Dong Chen
Published: September 2021

Polarization encoding has been extensively used in quantum key distribution (QKD) implementations along free-space links. However, the calculation model to characterize channel transmittance and quantum bit error rate (QBER) for free-space QKD has not been systematically studied. As a result, it is often assumed that misalignment error is equal to a fixed value, which is not theoretically rigorous. In this paper, we investigate the depolarization and rotation of the signal beams resulting from spatially-dependent polarization effects of the use of curved optics in an off-axis configuration, where decoherence can be characterized by the Huygens–Fresnel principle and the cross-spectral density matrix (CSDM). The transmittance and misalignment error in a practical free-space QKD can thus be estimated using the method. Furthermore, the numerical simulations clearly show that the polarization effect caused by turbulence can be effectively mitigated when maintaining good beam coherence properties.
Supporting Indigenous Students in Science and STEM Education: A Systematic Review
Authors: Jin Qingna
Published: September 2021

There are a growing number of education programs in science and STEM education with the aim of improving educational outcomes for Indigenous students who have long been underrepresented in current education systems. The aim of this study is to systematically review empirical research from 2011 to 2020 that reported programs to support Indigenous students in science and STEM education. A total of 24 studies were included in this review. These programs involved student participants from all K to 12 grade levels and occurred in both formal and informal contexts. Most of the programs employed multifaced approaches, and cultural relevance and scientific inquiry practice were the two main features of the programs. All the programs had reported positive outcomes in relation to Indigenous students’ science learning, understanding of their own cultures and traditions, and/or the complementarity of Western science and Indigenous knowledge.
A Flexible Future Education Model—Strategies Drawn from Teaching during the COVID-19 Pandemic
Authors: Srinivasan Seshasai , Ramos Juan Antonio Lopez , Muhammad Nasim
Published: September 2021

As they emerge from the pandemic, universities worldwide are evaluating the adaptations in the education sector during the pandemic and determining their course of action for the future. In this work, drawing on the lessons from four courses across two different universities, a survey of over 300 students, and the literature, we present strategies for successfully implementing a flexible blended education format. The survey revealed that the performance of the cohort taking the course during the pandemic performed nearly the same as the cohorts that took the courses before the pandemic. However, the students did not prefer an entirely virtual format, felt that their social wellbeing was impacted, and preferred a hybrid education model with a lot of supplementary learning material. As a key contribution of this work, we have identified and elaborate on four key pillars for a flexible blended education format, namely, course design, pedagogical strategies incorporating active learning and providing a sense of online community, infrastructure for delivery and training, and incorporating activities that support student wellbeing.