bluuebunny/crossref_metadata_2025 huggingface dataset:
Monitoring Groundwater Health Using Citizen Scientists in Semi‐Arid Regional Australia
Authors: Korbel Kathryn L. , Hose Grant C.
Published: April 2024

Citizen science (CS) around the world is undergoing a resurgence, potentially due to the utilization of new technologies and methods to capture information, such as data and photo entry via mobile phone apps. CS has been used in aquatic ecology for several decades, however the use of volunteers to collect data in groundwaters has rarely occurred. Groundwater research, particularly groundwater ecosystems, is unevenly distributed across the world, limiting our knowledge of these ecosystems and their functions. Here, we engaged six volunteer farmers in semi‐arid region of north‐western New South Wales, Australia to participate in an assessment of groundwater health using privately owned wells. Volunteers were supplied with sampling kits and instructions on sampling methods. Data retrieved indicated the health of the groundwater ecosystems, simultaneously providing information on water quality and groundwater biota present within the farm aquifers. Diverse stygofauna were collected from the trial, which reflected historical records of stygofauna within the same catchment indicating the viability of using citizen scientist for data collection. The citizen science project not only aided the collection of data and assessment of groundwater health, but also provided a tool for education, attracting media attention which furthered the education to a national audience. The amount of data still required to understand groundwater ecosystems, combined with the urgency to manage these environments, suggests that citizen scientists may complement the efforts of scientists around the globe to establish the impacts and consequences of human activities on this resource.
The rise of the remote metropolitan branch campus – Definitions, motivations and models
Authors: Hickey Rob , Healey Nigel
Published: April 2024

This paper explores the concept of the university remote metropolitan branch campus (RMBC). Drawing on approaches used to frame international branch campuses, it proposes a first definition for an RMBC, distinguishing it from a wider group of domestic ‘Satellite Campuses’ that includes multi‐campus universities within regions. Using interviews with 10 RMBC directors in London, it explores motivations for their creation and approaches used to organise and resource these ventures, as well as to attract and teach students. It concludes that the dominant reasons for creating RMBCs relate to financial diversification, reputation, reach and business engagement, but that in some cases the repositioning of an RMBC has been strategically reactive in response to external changes in its operating environment. Whilst there is evidence for a range of organisational models amongst London‐based RMBCs, there are key commonalities around the students attracted, the programmes offered and approaches to teaching and learning. RMBCs in London are growing and thriving, but face challenges from market entrants and uncertain future UK Government policy.
Presence of focal usual interstitial pneumonia is a key prognostic factor in progressive pulmonary fibrosis
Authors: Tsushima Yukio , Okoshi Ethan N , Ishijima Sousuke , Bychkov Andrey , Lami Kris , Morimoto Shimpei , Yamano Yasuhiko , Kataoka Kensuke , Johkoh Takeshi , Kondoh Yasuhiro , Fukuoka Junya
Published: April 2024

Aims

Progressive pulmonary fibrosis (PPF) is a newly recognised clinical phenotype of interstitial lung diseases in the 2022 interstitial pulmonary fibrosis (IPF) guidelines. This category is based entirely on clinical and radiological factors, and the background histopathology is unknown. Our objective was to investigate the histopathological characteristics of PPF and to examine the correlation between usual interstitial pneumonia (UIP) and prognosis in this new disease type. We hypothesised that the presence of UIP‐like fibrosis predicts patients’ survival in PPF cases.

Methods and results

We selected 201 cases fulfilling the clinical criteria of PPF from case archives. Cases diagnosed as IPF by a multidisciplinary team were excluded. Whole slide images were evaluated by three pathologists who were blinded to clinical and radiological data. We measured areas of UIP‐like fibrosis and calculated what percentage of the total lesion area they occupied. The presence of focal UIP‐like fibrosis amounting to 10% or more of the lesion area was seen in 148 (73.6%), 168 (83.6%) and 165 (82.1%) cases for each pathologist, respectively. Agreement of the recognition of UIP‐like fibrosis in PPF cases was above κ = 0.6 between all pairs. Survival analysis showed that the presence of focal UIP‐like fibrosis correlated with worsened survival under all parameters tested (P < 0.001).

Conclusions

The presence of UIP‐like fibrosis is a core pathological feature of clinical PPF, and its presence within diseased areas is associated with poorer prognosis. This study highlights the importance of considering the presence of focal UIP‐like fibrosis in the evaluation and management of PPF.
Exploring p53 protein expression and its link to TP53 mutation in myelodysplasia‐related malignancies—Interpretive challenges and potential field of applications
Authors: Bedekovics Judit , Madarász Kristóf , Mokánszki Attila , Molnár Sarolta , Mester Ágnes , Miltényi Zsófia , Méhes Gábor
Published: April 2024

Aims

TP53 alterations have a significant prognostic effect in myeloid neoplasms. Our objective was to investigate the TP53 gene mutation status, p53 protein expression and their relationship in dysplasia‐related myeloid neoplasms with varying levels of myeloblast counts.

Methods and results

A total of 76 bone marrow biopsy samples with different blast counts were analysed. Total and strong (3+) p53 expression was determined. Dual immunohistochemical staining was performed to determine the cell population associated with p53 expression. NGS analysis was performed using the Accel‐Amplicon Comprehensive TP53 panel. Both p53 expression and TP53 VAF showed a significant correlation with the myeloblast ratio (P < 0.0001); however, p53 expression was also present in other cell lineages. The VAF value exhibited a significant correlation with p53 expression. A high specificity (0.9800) was observed for TP53 mutation using the ≥ 10% strong (3+) p53 cut‐off value, although the sensitivity (0.4231) was low.

Conclusions

Strong (3+) p53 expression using a ≥ 10% cut‐off value accurately predicts TP53 mutation but does not reveal the allelic state. The p53 expression is significantly influenced by myeloblast count, and histological interpretation should consider the presence of intermixed non‐neoplastic marrow cells with varying physiological p53 expression.
Identification of tumour regression in neoadjuvantly treated pancreatic cancer is based on divergent and nonspecific criteria
Authors: Holm Maia Blomhoff , Lenggenhager Daniela , Detlefsen Sönke , Sántha Petra , Verbeke Caroline Sophie
Published: April 2024

Aims

Following the increased use of neoadjuvant therapy for pancreatic cancer, grading of tumour regression (TR) has become part of routine diagnostics. However, it suffers from marked interobserver variation, which is mainly ascribed to the subjectivity of the defining criteria of the categories in TR grading systems. We hypothesized that a further cause for the interobserver variation is the use of divergent and nonspecific morphological criteria to identify tumour regression.

Methods and Results

Twenty treatment‐naïve pancreatic cancers and 20 pancreatic cancers treated with neoadjuvant chemotherapy were reviewed by three experienced pancreatic pathologists who, blinded for treatment status, categorized each tumour as treatment‐naïve or neoadjuvantly treated, and annotated all tissue areas they considered showing tumour regression. Only 50%–65% of the cases were categorized correctly, and the annotated tissue areas were highly discrepant (only 3%–41% overlap). When the prevalence of various morphological features deemed to indicate TR was compared between treatment‐naïve and neoadjuvantly treated tumours, only one pattern, characterized by reduced cancer cell density and prominent stroma affecting a large area of the tumour bed, occurred significantly more frequently, but not exclusively, in the neoadjuvantly treated group. Finally, stromal features, both morphological and biological, were investigated as possible markers for tumour regression, but failed to distinguish TR from native tumour stroma.

Conclusion

There is considerable divergence in opinion between pathologists when it comes to the identification of tumour regression. Reliable identification of TR is only possible if it is extensive, while lesser degrees of treatment effect cannot be recognized with certainty.
TORT LIABILITY AND UNAWARENESS
Authors: Chakravarty Surajeet , Kelsey David , Teitelbaum Joshua C.
Published: April 2024

We explore the implications of unawareness for tort law. We study cases where injurers and victims initially are unaware that some acts can yield harmful consequences, or that some acts or harmful consequences are even possible, but later become aware. We model unawareness by Reverse Bayesianism. We compare the two basic liability rules of Anglo‐American tort law, negligence and strict liability, and argue that negligence has an important advantage over strict liability in a world with unawareness—negligence, through the stipulation of due care standards, spreads awareness about the updated probability of harm.
Intention to use telemedicine services during a health crisis: A motivation‐opportunity‐ability theory approach
Authors: Atmaja Ferry Tema , Wu Cedric Hsi‐Jui , Guttena Revanth Kumar , Honora Andreawan
Published: April 2024

Telemedicine, despite its longstanding presence, has emerged as a valuable resource amidst health crises. Yet, research on individuals' willingness to embrace telemedicine in such circumstances remains scarce. This article employs the Motivation–Opportunity–Ability theory to construct a novel framework for investigating how motivational, opportunity‐related, and ability‐related factors influence individuals' intention to use telemedicine during a health crisis. A sample of 468 respondents from Indonesia participated in an online survey. The data were analyzed using covariance‐based structural equation modeling and the Hayes PROCESS macro. The findings revealed that contamination avoidance, telemedicine usability, and privacy concerns significantly influence the intention to use telemedicine services. Moreover, the findings also confirmed the moderated moderating effect of e‐health literacy and technology optimism on the relationship between contamination avoidance and intention to use telemedicine services. By deepening our understanding of the factors shaping individuals' intention to use telemedicine services, this study makes valuable academic contributions to the field of telemedicine adoption literature. Additionally, it provides practical insights for telemedicine providers aiming to enhance individuals' intention to use telemedicine services during critical health situations.
Feasibility of using a combination of staphylococcal superantigen‐like proteins 3, 7 and 11 in a fusion vaccine for Staphylococcus aureus
Authors: Chan Janlin Ying Hui , Clow Fiona , Pearson Victoria , Langley Ries J , Fraser John D , Radcliff Fiona J
Published: April 2024

Staphylococcus aureus is a significant bacterial pathogen in both community and hospital settings, and the escalation of antimicrobial‐resistant strains is of immense global concern. Vaccination is an inviting long‐term strategy to curb staphylococcal disease, but identification of an effective vaccine has proved to be challenging. Three well‐characterized, ubiquitous, secreted immune evasion factors from the staphylococcal superantigen‐like (SSL) protein family were selected for the development of a vaccine. Wild‐type SSL3, 7 and 11, which inhibit signaling through Toll‐like receptor 2, cleavage of complement component 5 and neutrophil function, respectively, were successfully combined into a stable, active fusion protein (PolySSL7311). Vaccination of mice with an attenuated form of the PolySSL7311 protein stimulated significantly elevated specific immunoglobulin G and splenocyte proliferation responses to each component relative to adjuvant‐only controls. Vaccination with PolySSL7311, but not a mixture of the individual proteins, led to a > 102 reduction in S. aureus tissue burden compared with controls after peritoneal challenge. Comparable antibody responses were elicited after coadministration of the vaccine in either AddaVax (an analog of MF59) or an Alum‐based adjuvant; but only AddaVax conferred a significant reduction in bacterial load, aligning with other studies that suggest both cellular and humoral immune responses are necessary for protective immunity to S. aureus. Anti‐sera from mice immunized with PolySSL7311, but not individual proteins, partially neutralized the functional activities of SSL7. This study confirms the importance of these SSLs for the survival of S. aureus in vivo and suggests that PolySSL7311 is a promising vaccine candidate.
The increasing costs of medicines and their implications for patients, physicians and the health system
Authors: Ghinea Narcyz
Published: April 2024

Most new medicines entering the market are high‐cost speciality drugs. These drugs can cost tens to hundreds of thousands of dollars per course of treatment and in some cases millions of dollars per dose. Approximately half of all spending on medicines is projected to target only 2–3% of patients, raising important questions about resource allocation. While there is no doubt that breakthrough innovations have transformed clinical care in some disciplines, it is also true that cost is becoming one of the primary barriers to treatment access and that many new medicines do not provide value commensurate with their prices. This article examines pricing trends, the reasons for high prices and their implications for access and clinical practice.
Risk factors associated with the occurrence of avulsion in the primary incisors: A case–control study
Authors: Negro Bianca Del , Hermann Nuno Vibe , Lauridsen Eva , Mendes Fausto Medeiros , Wanderley Marcia Turolla
Published: April 2024

Background

Identifying factors associated with the occurrence of primary tooth avulsion is essential to promote prevention strategies.

Aim

To investigate the risk factors associated with primary incisor avulsion and variables associated with post‐avulsion sequelae in the permanent successor.

Design

This case–control study comprised 407 children (cases) with primary incisor avulsion and 407 children (controls) with other traumatic dental injuries (TDI). The association between explanatory variables and avulsion was evaluated through logistic regression. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated. Poisson regression analyses between potential explanatory variables and sequelae were run, from which relative risks (RR) and corresponding 95% CI were estimated.

Results

TDI caused by moderate falls (OR = 2.20; 95% CI = 1.47 to 3.27), affecting lateral incisors (OR = 10.10; 95% CI = 3.89 to 26.54) and the lower arch (OR = 9.54; 95% CI = 3.15 to 28.85), were associated with primary incisor avulsion. Moreover, children with previous severe TDI, anterior open bite, and anterior crossbite had higher odds of primary incisor avulsion. Children who suffered from any primary tooth avulsion (RR = 2.68; 95% CI = 1.82 to 3.95) had a higher risk of sequelae in the permanent successors. The risk for sequelae in the permanent teeth was significantly greater for younger children under age 2 years than for children ages 3 to 5 years.

Conclusion

Risk factors for primary incisor avulsion are related to the severity of the fall, tooth position, history of previous TDI, and malocclusion. Furthermore, avulsion increases the risk of sequelae in the permanent successors.
RETRACTED: Comparing surgical site wound infection after laparoscopic and open radical cystectomies in patients with bladder cancer
Authors: Luo Hua‐Ming , Xiong Yong‐Jiang
Published: April 2024

This study comprehensively compared the effects of laparoscopic and open radical cystectomies on postoperative wound infections and complications in patients with bladder cancer. We conducted a systematic search for relevant studies in PubMed, Embase, Google Scholar, Cochrane Library, China National Knowledge Infrastructure, and Wanfang databases, from database inception to October 2023. Two researchers independently screened the literature, extracted data, and assessed the quality based on the inclusion and exclusion criteria. Data analysis was performed using Stata 17.0 software. Overall, 16 studies involving 1427 patients with bladder cancer were included. The analysis revealed that, compared with open radical cystectomy, laparoscopic radical cystectomy significantly reduced the incidence of wound infections (odds ratio [OR] = 0.38, 95% confidence interval [CI]: 0.23–0.64, p < 0.001) and complications (OR = 0.35, 95%CI: 0.26–0.47, p < 0.001) and significantly shortened the hospital stay duration (standardised mean difference [SMD] = −1.85, 95%CI: −2.34 to −1.36, p < 0.001). Thus, this study determined that laparoscopic radical cystectomy for the treatment of bladder cancer effectively reduced the occurrence of wound infections and complications, and significantly shortened the patient's hospital stay, demonstrating notable therapeutic effectiveness worthy of clinical application.
Characteristics and outcomes of diabetic foot ulcers treated with surgical debridement and standardized wound care
Authors: Moghaddam Ahmadi Moein , Ashoobi Mohammad Taghi , Darabi Zohreh , Ramezannezhad Hossein , Moghaddam Ahmadi Mahta
Published: April 2024

Diabetic foot ulcers (DFUs) pose a significant clinical challenge, often leading to amputations and hospitalisation. This study aimed to investigate the characteristics and outcomes of DFUs treated with surgical debridement and standardised wound care. This descriptive cross‐sectional study focused on diabetic patients with appropriate vascular conditions, as determined by an Ankle Brachial Index >0.9. Based on their infection status, participants were admitted to Poursina Hospital in Rasht, Iran, and subjected to initial supportive measures, antibiotic therapy and surgical debridement. The study incorporated primary treatment with wet bandages, silver spray and fibrinolysin ointment. Statistical analysis employed SPSS 22 software. Most patients were male (54.7%) and under 60 years old (50.7%). Overweight status was prevalent in 69.3% of diabetic ulcer patients, amongst whom 48% underwent wrist debridement. The 64% and 36% of the cases had grade III and grade II Texas index. Moreover, 96% of patients exhibited signs of infection and were classified as Stage Texas B. Reoperation was necessary for 34.7% of patients. The mean hospital stay was 8.5 ± 7.55 days, and the average recovery time was 15.2 ± 15.19 days. Out of 75 patients, 10 were unable to return to limb function due to disability. In this study, around one‐third of patients required secondary repair with grafts and flaps. A small number of them were unable to recover because of underlying disability, and the mean recovery time in other cases was 24 days. Future studies should follow up with patients for longer periods to assess long‐term therapeutic outcomes and quality of life.
Analysing predictors of surgical site infections in patients undergoing emergency surgery for traumatic pulmonary haemorrhage
Authors: Zhang Jie , Pan Chengwen
Published: April 2024

Identifying predictors for surgical site infections (SSIs) after emergency surgical treatment for traumatic pulmonary haemorrhage (TPH) is crucial for improving patient outcomes. This study aims to ascertain these predictors. In this comprehensive retrospective study, conducted from January 2020 to December 2023 at our institution, 75 patients were analysed, including a case group of 25 patients with SSIs and a control group of 50 without SSIs post‐TPH surgery. Inclusion criteria focused on patients aged 18 and above undergoing thoracotomy or minimally invasive thoracic surgery for TPH. Exclusion criteria included compromised immune systems, chronic pulmonary diseases, prior thoracic surgery or active infections at admission. We assessed several predictors: anaemia; operation time over 2 h; hospital stay over 5 days; intraoperative blood loss exceeding 500 mL; body mass index (BMI) ≥25 kg/m2; age ≥ 50 years; use of surgical drains; the presence of open wounds; diabetes mellitus and non‐prophylactic antibiotic use. Statistical analysis involved univariate and multivariate logistic regression, using SPSS Version 27.0. Univariate analysis revealed significant associations between SSIs and surgical drain placement, diabetes mellitus, open wounds and non‐prophylactic antibiotic use (p < 0.01). Multivariate analysis confirmed these factors as significant predictors of SSIs, with notable odds ratios. Other variables like anaemia, extended hospital stay, excessive intraoperative blood loss, older age and higher BMI did not significantly predict SSIs. Significant predictors for SSIs following TPH surgery include surgical drain placement, diabetes mellitus, open wounds and non‐prophylactic antibiotic use. Identifying and managing these risks is crucial in clinical practice to reduce SSIs incidence and improve patient outcomes.
Enhancing the microwave absorption performance of graphite/Al2O3 composites through manipulating sheet resistance
Authors: Yang Zhaoning , Wang Jing , Yang Zihao , Shu Xiaoxin , Ren Wei , Wang Jie
Published: April 2024

The graphite/Al2O3 composite was prepared using plasma spray technology, the material design, experimental methods, and process flow are introduced. The microstructure, chemical composition, and complex permittivity of the composite are investigated, and its performance to absorb electromagnetic waves is studied. The mechanism of interaction between chemical composition, microstructure, complex permittivity, and absorption performance is analyzed. Furthermore, a microwave absorber composed of graphite/Al2O3 and sheet resistance, and performance is discussed. The reflectivity of the composite coating with different periods and sizes and resistances is analyzed. The impedance and reflectivity of the designed absorber that contains sheet resistance are discussed, and its absorption mechanism is explained in detail. The results demonstrate that the microwave absorption performance of graphite/Al2O3 coating is enhanced by electromagnetic resonance of the sheet resistance. The designed absorber exhibits good wave‐absorbing performance and stability at the frequency of the 8.2–18 GHz range. When the sample thickness is 2.3 mm, the absorber achieves a reflection loss (RL) value of −16.8 dB at 9.8 GHz and a wide effective absorption bandwidth (EAB) of 9.8 GHz. It means that the RL below −10 dB can be obtained in the whole X band and Ku bands. These results proved that the EAB can be tuned and enhanced by manipulating sheet resistance.
A Horizon‐Based Decomposition of Mutual Fund Value Added Using Transactions
Authors: BINSBERGEN JULES VAN , HAN JUNGSUK , RUAN HONGXUN , XING RAN
Published: April 2024

We decompose mutual fund value added by the length of funds' holdings using transaction‐level data. We motivate our decomposition with a model featuring horizon‐specific investment ideas, where short‐term ideas are less scalable because the associated trades cannot be spread over time. Fund turnover correlates negatively with the horizon over which value is added and positively with price impact costs. As predicted, holdings of high‐turnover funds add a substantial amount of value in the first two weeks, of which more than 80% is earned on Federal Open Market Committee (FOMC) and earnings announcement days. Holdings of low‐turnover funds add value only over longer horizons.
The effect of sleep position on sleep bruxism in adults with obstructive sleep apnea
Authors: Kuang Boyuan , Lobbezoo Frank , Li Deshui , Hilgevoord Antonius A. J. , de Vries Nico , Aarab Ghizlane
Published: April 2024

Background

Most of the respiratory events in adults with obstructive sleep apnea (OSA) occurs in supine position. It has been reported that the contraction of masseter muscles is dependent on the occurrence of arousals rather than on the occurrence of respiratory events.

Objectives

This study had two aims: (1) to compare the rhythmic masticatory muscle activity (RMMA) index in supine position (RMMA_sup) and in non‐supine positions (RMMA_nsup) in adults with OSA; and (2) to determine the associations between RMMA index in both supine position and non‐supine positions on the one hand, and several demographic and polysomnographic variables on the other hand.

Methods

One hundred OSA participants (36 females and 64 males; mean age = 50.3 years (SD = 10.5)) were selected randomly from among patients with a full‐night polysomnographic recording. RMMA_sup index and RMMA_nsup index were compared using Mann–Whitney U‐test. Multivariate linear regression analyses were used to predict RMMA index both in supine and non‐supine positions based on several demographic and polysomnographic variables.

Results

In patients with OSA, the RMMA_sup index was significantly higher than the RMMA_nsup index (p < .001). RMMA_sup index was significantly associated with the arousal index (p = .002) and arousal index in supine position (p < .001). RMMA_nsup index was only significantly associated with the arousal index in non‐supine positions (p = .004).

Conclusion

Within the limitations of this study, RMMAs occur more frequently in supine position than in non‐supine positions in patients with OSA. In both sleep positions, RMMAs are associated with arousals.
Added sugar in coffee beverages: A study with a sample of Brazilian consumers of sweetened and unsweetened coffee
Authors: da Portela Claudimara da Silva , Viencz Thayna , dos Santos Karen Laíssa Balbino , Filho Tarcísio Lima , de Benassi Marta de Toledo
Published: April 2024

This study aimed to determine the compromised acceptance threshold (CAT) and the hedonic rejection threshold (HRT) for consumers of sweetened coffee to propose a strategy to reduce the sucrose addition by studying the behavior of consumers of sweetened and unsweetened coffee. From the sample that showed the highest acceptance (control sample, with 12% added sucrose), it was possible to reduce the sucrose addition by up to 18% without compromising acceptance (CAT = 9.76%) and by up to 52% without promoting the rejection of the beverage (HRT = 5.61%). Consumers who were reducing the sucrose addition exhibited a reduction in CAT (6.54%). Consumers of sweetened and unsweetened coffee had different perceptions regarding the sweetened beverage, thus, research involving coffee consumption should be segmented between these two groups. As a strategy, we suggest that the reduction of sucrose should be gradual and associated with information about its health benefits.

Practical Applications

The results are relevant for those interested in understanding the habits of consumers of sweetened and unsweetened coffee and providing an estimate of the tolerance of consumers of sweetened beverages when reducing the sucrose content in coffee beverages. Considering the volume and regularity of coffee consumption by Brazilian consumers, these findings can contribute to the development of a strategy to reduce sugar consumption. This information also supports the production sector in developing ready‐to‐drink beverages, prepared by industries and coffee shops or available in vending machines, indicating that the concentration of added sugar provided can be reduced, as long as a sufficient amount is maintained not to impair the acceptance of the beverage. Additionally, the data are important for sensory analysis researchers, enabling them to better adapt coffee beverage tasting conditions to consumer expectations and, thus, obtain more reliable results.
Pharmacokinetics of long‐acting cephapirin and cloxacillin after intramammary administration in dairy goats
Authors: Buckley Michelle P. , Hayman Kristen P. , Burns Laura , Schrunk Dwayne , Gorden Patrick J.
Published: April 2024

Determining the pharmacokinetics of intramammary antimicrobials in goats can assist in predicting appropriate meat and milk withdrawal intervals for drugs that are effective at treating subclinical mastitis due to non‐aureus Staphylococci during the dry period. Twenty‐four healthy, lactating does were enrolled in this study. Half were administered 300 mg of cephapirin benzathine (ToMORROW, Boehringer Ingelheim Vetmedica, Duluth, GA) via intramammary infusion into each half of the udder. The remaining does had 500 mg cloxacillin benzathine (Orbenin DC, Merck & Co., Rahway, NJ) administered per half. Plasma was collected before treatment and for 7 days post‐treatment followed by analysis via liquid chromatography with tandem mass spectroscopy. Pharmacokinetic parameters were determined using noncompartmental methods via commercial software (MonolixSuite). The mean maximum concentration (C
max) of cephapirin of 0.073 μg/mL was noted at 7.06 h post‐administration (T
max). The area under the plasma concentration curve based on the final sampling point (AUClast) was 1.06 h × μg/mL. The mean residence time until the final sampling point (MRTlast) was 13.55 h. Mean terminal half‐life (T
½) of cephapirin was 6.98 h. In CLOX does, C
max was 0.074 μg/mL with a T
max of 18 h, AUClast was 5.71 h × μg/mL, T
½ was 77.45 h, and MRTlast was 65.36 h. Despite both products being formulated with benzathine salts, marked differences were noted in pharmacokinetic parameters including AUC, T
1/2, and MRTlast. This data will be used to plan sampling schedules for milk and tissue residue depletion studies for both products.
The acceptability of a donor human milk bank and donated human milk among mothers in Limpopo Province, South Africa
Authors: Mampane Tebogo , Wolvaardt Jacqueline E.
Published: April 2024

Breastfeeding is a crucial public health approach that reduces infant morbidity and mortality by providing essential nutrients and antibodies, and breast milk is easily digested. Breastfeeding and donated milk serve as a preventative measure against necrotising enterocolitis. Additionally, they protect against viruses and nosocomial sepsis. When a birthing parent's own milk is unavailable, alternative enteral nutrition for preterm or low‐birth‐weight infants is either donor human milk (DHM) or artificial formula. This study aimed to understand mothers' acceptance of the donor human milk bank (DHMB) and DHM. A qualitative phenomenological study was conducted in Limpopo Province, South Africa. The study used purposive sampling to select 23 mothers in postnatal and neonatal wards. Data collection was via in‐depth interviews using a semistructured interview guide. Manual data analysis using an interpretative phenomenological analysis (IPA) framework was used to coding. Concepts were grouped to generate themes. Three themes and nine subthemes were generated: (1) DHMBs (2) cultural perspective of DHMB, and (3) health considerations of DHM. Participants were unaware of the DHMB. Hesitancy in accepting DHM due to fear of contracting HIV was observed. Cultural beliefs are an influencing factor for use, while donation was driven by altruistic reasons, preventing waste, helping others and having previously benefited from DHM. The study found that mothers are willing to donate human milk. Willingness to donate can be increased by raising awareness about DHMB and addressing culture and safety concerns at antenatal clinics.
Pedagogies of discomfort in the world language classroom: Ethical tensions and considerations for educators
Authors: Porto Melina , Zembylas Michalinos
Published: April 2024

The purpose of this article is to examine the ethical tensions and considerations that arise in the world language classroom from using pedagogies of discomfort. Although pedagogies of discomfort have mostly been seen through a positive lens in the literature for engaging students with difficult issues in the classroom, there are ethical concerns, particularly in relation to the harm that students might experience. To illustrate these ethical concerns and their implications in the world language classroom, we draw on data from a number of projects in which pedagogies of discomfort have been used in university classrooms. The analysis of examples shows that while some sort of ethical violence is inevitable, there are pedagogical ways to minimize the harm on students. The article concludes by raising further ethical and pedagogical questions for exploration in the context of using pedagogies of discomfort in the world language classroom.
Comparison of dermoscopic characteristics on toenail onychomycosis in psoriatic and non‐psoriatic patients: A prospective study
Authors: Wang Shiqi , Chen Wei , Liu Fang , Li Ruoyu
Published: April 2024

Background

Psoriatic patients may experience the coexistence of onychomycosis (OM). However, the evaluation of OM in psoriatics has been hindered by potential clinical differences from OM in non‐psoriatics.

Objective

To assess and compare dermoscopic features between toenail OM in psoriatic and in non‐psoriatic patients.

Patients and Methods

Between September 2020 and September 2023, dermoscopy was conducted on 183 affected toenails by OM in psoriatics and 232 affected toenails by OM in non‐psoriatics in two centres. The dermoscopic characteristics were compared using the Chi‐squared test.

Results

Among toenail OM cases in psoriatic subjects, the most prevalent dermoscopic features included pitting (147/183, 80.33%) and subungual hyperkeratosis (118/183, 64.48%). Conversely, toenail OM in non‐psoriatics was characterized by subungual hyperkeratosis (175/232, 75.43%) and nail spikes (139/232, 59.91%). Comparative analysis revealed a significantly higher occurrence of pitting (80.33% vs. 15.96%, p < .001), periungual telangiectasis (22.40% vs. 4.74%, p < .001), oil patches (12.57% vs. 0.43%,p < .001) and transverse grooves (43.72% vs. 28.45%,p < .01) in toenail OM in psoriatics. Furthermore, toenail OM in psoriatics exhibited a significantly lower frequency of yellow structureless area (13.11% vs. 42.67%, p < .001), nail spikes (43.17% vs. 59.91%, p < .01), ruin appearance of sulphur nugget (8.20% vs. 31.03%, p < .001), dotted/blocky haemorrhage (6.01% vs. 20.69%,p < .001) and partial onycholysis (32.79% vs. 46.98%, p < .01).

Conclusions

Dermoscopic features of toenail OM in psoriatic and non‐psoriatic patients exhibit notable differences. OM in psoriatics shows a higher frequency of pitting and periungual telangiectasis, while a lower frequency of yellow structureless areas and nail spikes under dermoscopy.
Ethylene controls three‐dimensional growth involving reduced auxin levels in the moss Physcomitrium patens
Authors: Wang Yidong , Jiang Lanlan , Kong Dongdong , Meng Jie , Song Meifang , Cui Wenxiu , Song Yaqi , Wang Xiaofan , Liu Jiao , Wang Rui , He Yikun , Chang Caren , Ju Chuanli
Published: April 2024

The conquest of land by plants was concomitant with, and possibly enabled by, the evolution of three‐dimensional (3D) growth. The moss Physcomitrium patens provides a model system for elucidating molecular mechanisms in the initiation of 3D growth. Here, we investigate whether the phytohormone ethylene, which is believed to have been a signal before land plant emergence, plays a role in 3D growth regulation in P. patens.

We report ethylene controls 3D gametophore formation, based on results from exogenously applied ethylene and genetic manipulation of PpEIN2, which is a central component in the ethylene signaling pathway.

Overexpression (OE) of PpEIN2 activates ethylene responses and leads to earlier formation of gametophores with fewer gametophores produced thereafter, phenocopying ethylene‐treated wild‐type. Conversely, Ppein2 knockout mutants, which are ethylene insensitive, show initially delayed gametophore formation with more gametophores produced later. Furthermore, pharmacological and biochemical analyses reveal auxin levels are decreased in the OE lines but increased in the knockout mutants.

Our results suggest that evolutionarily, ethylene and auxin molecular networks were recruited to build the plant body plan in ancestral land plants. This might have played a role in enabling ancient plants to acclimate to the continental surfaces of the planet.
Impact of insulin sensitization on metabolic and fertility outcomes in women with polycystic ovary syndrome and overweight or obesity—A systematic review, meta‐analysis, and meta‐regression
Authors: Samarasinghe Suhaniya N. S. , Ostarijas Eduard , Long Matthew J. , Erridge Simon , Purkayastha Sanjay , Dimitriadis Georgios K. , Miras Alexander D.
Published: April 2024

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in reproductive‐age women. This systematic review, meta‐analysis, and meta‐regression aims to compare the effect of insulin sensitizer pharmacotherapy on metabolic and reproductive outcomes in women with PCOS and overweight or obesity. We searched online databases MEDLINE via OVID, EMBASE, Clinicaltrials.gov, and EudraCT for trials published from inception to November 13, 2023. Inclusion criteria were double‐blind, randomized controlled trials in women diagnosed with PCOS, body mass index (BMI) ≥ 25 kg/m2, which reported metabolic or reproductive outcomes. The intervention was insulin sensitization pharmacotherapy versus placebo or other agents. The primary outcomes were changes from baseline BMI, fasting blood glucose, and menstrual frequency. Nineteen studies were included in this review. Metformin had the most significant effect on the fasting plasma glucose and body mass index. Insulin sensitizer pharmacotherapy significantly reduced fasting plasma glucose, body mass index, fasting serum insulin, HOMA‐IR, sex hormone binding globulin, and total testosterone, but the effect size was small. There was a lack of menstrual frequency and live birth data. The results indicate a role for insulin sensitizers in improving the metabolic and, to a lesser degree, reproductive profile in these women. Further research should examine insulin sensitizers' effects on objective measures of fecundity.
Site‐directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds
Authors: Trinh Mai Duy Luu , Visintainer Davide , Günther Jan , Østerberg Jeppe Thulin , da Fonseca Rute R. , Fondevilla Sara , Moog Max William , Luo Guangbin , Nørrevang Anton F. , Crocoll Christoph , Nielsen Philip V. , Jacobsen Sven‐Erik , Wendt Toni , Bak Søren , López‐Marqués Rosa Laura , Palmgren Michael
Published: April 2024

Climate change may result in a drier climate and increased salinization, threatening agricultural productivity worldwide. Quinoa (Chenopodium quinoa) produces highly nutritious seeds and tolerates abiotic stresses such as drought and high salinity, making it a promising future food source. However, the presence of antinutritional saponins in their seeds is an undesirable trait. We mapped genes controlling seed saponin content to a genomic region that includes TSARL1. We isolated desired genetic variation in this gene by producing a large mutant library of a commercial quinoa cultivar and screening the library for specific nucleotide substitutions using droplet digital PCR. We were able to rapidly isolate two independent tsarl1 mutants, which retained saponins in the leaves and roots for defence, but saponins were undetectable in the seed coat. We further could show that TSARL1 specifically controls seed saponin biosynthesis in the committed step after 2,3‐oxidosqualene. Our work provides new important knowledge on the function of TSARL1 and represents a breakthrough for quinoa breeding.
Compression Therapy after Thermal Ablation of Varicose Veins: A Meta‐Analysis
Authors: Su Li , Zhang Ling , Yuan Tao , Ji Li‐Ping , Liu Meng , Li Rong‐Zhen , Lv Hai‐Ling , Guo Shu‐Yun
Published: April 2024

Objective

To investigate whether compression therapy after thermal ablation of varicose veins can improve the prognosis of patients.

Methods

Systematic research were applied for Chinese and English electronic databases(PubMed, Web of Science, Cochrane Library, CNKI, Wanfang, VIP Databases). Eligible prospective studies that comparing the efficacy of compression therapy and non‐compression therapy on patients after thermal ablation of varicose veins were included. The interest outcome such as pain, quality of life (QOL), venous clinical severity score (VCSS), time to return to work and complications were analyzed.

Results

10 studies were of high quality, and randomized controlled trials involving 1,545 patients met the inclusion criteria for this study. At the same time, the meta‐analysis showed that the application of compression therapy improved pain (SMD: ‐0.51, 95% CI: ‐0.95, ‐0.07) but exhibited no statistically significant effect on QOL (SMD: 0.04, 95% CI: ‐0.08, 0.16), VCSS (MD: ‐0.05, 95% CI: ‐1.19, 1.09), time to return to work (MD: ‐0.43, 95% CI: ‐0.90, 0.03), total complications (RR: 0.54, 95% CI: 0.27, 1.09), and thrombosis (RR: 0.71, 95% CI: 0.31, 1.62).

Conclusion

Compression therapy after thermal ablation of varicose veins can slightly relieve pain, but it has not been found to be associated with improvement in other outcomes.
Characterization of blue‐green blood leukocyte inclusions and accompanying clinical, hematologic, and serum biochemical changes in dogs
Authors: Sebastian Kimberley N. , Lucidi Cynthia A. , Scott Michael A.
Published: April 2024

Background

Lipofuscin‐like cytoplasmic inclusions have been reported in human blood neutrophils and monocytes but have not been described in dogs. In people, these “green granules of death” have been associated with moderate to severe hepatocellular injury and high mortality.

Objectives

To describe clinicopathologic abnormalities, diagnoses, and outcomes of dogs with greenish inclusions in blood neutrophils or monocytes, and to determine if the inclusions have features of lipofuscin.

Methods

Clinical cases were identified prospectively through routine evaluation of CBC samples. Leukocyte inclusions were characterized with routine staining and assessed for iron and autofluorescence. Additional cases were identified by examination of archived blood smears from dogs meeting search criteria for hepatocellular injury, and clinicopathologic findings were recorded.

Results

All 7 prospectively identified dogs with inclusions had inflammation and moderate to marked increases in serum alanine aminotransferase (ALT) activity, as did the 4 dogs identified from the 97 meeting retrospective search criteria. The inclusions were Prussian blue–negative (5/5) with broad‐spectrum autofluorescence (5/5) and the appearance of lipofuscin with and without Wright staining. Most clinical diagnoses involved hepatic disorders (5/7 prospective and 3/4 retrospective cases) or pancreatitis (3/7 prospective and 2/4 retrospective cases), and some involved both; 8 of 11 dogs died within 7 days of admission.

Conclusions

Blue‐green cytoplasmic inclusions uncommonly found in blood neutrophils ± monocytes of routine canine blood smears have stained and unstained properties of lipofuscin and suggest the presence of hepatocellular injury, often severe. Reporting these inclusions is recommended to guide clinical management.
Increasing Hamstring Range of Motion via Plantar Myofascial Release: A Critically Appraised Topic
Authors: Finley Alexandra , McDevitt Jane
Published: April 2024

Context: The concept of anatomical fascial trains and myofascial release are heavily researched topics independently. However, few studies have determined if myofascial release of remote areas can cause lengthening throughout more distal aspects of these fascial trains. Clinical Question: In healthy individuals, what is the effect of myofascial release on the plantar aspect of the foot in improving flexibility and hamstring length as determined by a sit-and-reach test? Clinical Bottom Line: The usage of myofascial release on the plantar aspect of the foot via a firm object has been shown to improve sit-and-reach distance. Each article included for analysis utilized similar methods of instructing self-myofascial release with regard to time as well as type of object used for the intervention. Though this was consistent between studies, the authors should have implemented secondary outcome measures. Despite the limitations to each of the studies analyzed, there is a Strength of Recommendation Taxonomy (SORT) Level B evidence to support the implementation self-myofascial release to the plantar fascia. This intervention should be considered a concurrent option for individuals with range of motion deficits or fascial restrictions in the upper thigh.
Apoptotic cell identity induces distinct functional responses to IL-4 in efferocytic macrophages
Authors: Liebold Imke , Al Jawazneh Amirah , Casar Christian , Lanzloth Clarissa , Leyk Stephanie , Hamley Madeleine , Wong Milagros N. , Kylies Dominik , Gräfe Stefanie K. , Edenhofer Ilka , Aranda-Pardos Irene , Kriwet Marie , Haas Helmuth , Krause Jenny , Hadjilaou Alexandros , Schromm Andra B. , Richardt Ulricke , Eggert Petra , Tappe Dennis , Weidemann Sören A. , Ghosh Sourav , Krebs Christian F. , A-Gonzalez Noelia , Worthmann Anna , Lohse Ansgar W. , Huber Samuel , Rothlin Carla V. , Puelles Victor G. , Jacobs Thomas , Gagliani Nicola , Bosurgi Lidia
Published: April 2024

Macrophages are functionally heterogeneous cells essential for apoptotic cell clearance. Apoptotic cells are defined by homogeneous characteristics, ignoring their original cell lineage identity. We found that in an interleukin-4 (IL-4)–enriched environment, the sensing of apoptotic neutrophils by macrophages triggered their tissue remodeling signature. Engulfment of apoptotic hepatocytes promoted a tolerogenic phenotype, whereas phagocytosis of T cells had little effect on IL-4–induced gene expression. In a mouse model of parasite-induced pathology, the transfer of macrophages conditioned with IL-4 and apoptotic neutrophils promoted parasitic egg clearance. Knockout of phagocytic receptors required for the uptake of apoptotic neutrophils and partially T cells, but not hepatocytes, exacerbated helminth infection. These findings suggest that the identity of apoptotic cells may contribute to the development of distinct IL-4–driven immune programs in macrophages.
Single body-coupled fiber enables chipless textile electronics
Authors: Yang Weifeng , Lin Shaomei , Gong Wei , Lin Rongzhou , Jiang Chengmei , Yang Xin , Hu Yunhao , Wang Jingjie , Xiao Xiao , Li Kerui , Li Yaogang , Zhang Qinghong , Ho John S. , Liu Yuxin , Hou Chengyi , Wang Hongzhi
Published: April 2024

Intelligent textiles provide an ideal platform for merging technology into daily routines. However, current textile electronic systems often rely on rigid silicon components, which limits seamless integration, energy efficiency, and comfort. Chipless electronic systems still face digital logic challenges owing to the lack of dynamic energy-switching carriers. We propose a chipless body-coupled energy interaction mechanism for ambient electromagnetic energy harvesting and wireless signal transmission through a single fiber. The fiber itself enables wireless visual–digital interactions without the need for extra chips or batteries on textiles. Because all of the electronic assemblies are merged in a miniature fiber, this facilitates scalable fabrication and compatibility with modern weaving techniques, thereby enabling versatile and intelligent clothing. We propose a strategy that may address the problems of silicon-based textile systems.
Sex differences in tissue immunity
Authors: Puttur Franz , Lloyd Clare M.
Published: April 2024

Androgen signaling skews skin immunity toward reduced inflammation in male mice