bluuebunny/crossref_metadata_2025 huggingface dataset:
Aortic Stenosis Phenotypes and Precision Transcatheter Aortic Valve Implantation
Authors: Khawaja Muzamil , Virk Hafeez Ul Hassan , Bandyopadhyay Dhrubajyoti , Rodriguez Mario , Escobar Johao , Alam Mahboob , Jneid Hani , Krittanawong Chayakrit
Published: June 2023

Patients with a clinical indication for aortic valve replacement can either undergo surgical aortic valve replacement (SAVR) or Transcatheter Aortic Valve Implantation (TAVI). There are many different factors that go into determining which type of replacement to undergo, including age, life expectancy, comorbidities, frailty, and patient preference. While both options offer significant benefits to patients in terms of clinical outcomes and quality of life, there is growing interest in expanding the indications for TAVI due to its minimally invasive approach. However, it is worth noting that there are several discrepancies in TAVI outcomes in regards to various endpoints, including death, stroke, and major cardiovascular events. It is unclear why these discrepancies exist, but potential explanations include the diversity of etiologies for aortic stenosis, complex patient comorbidities, and ongoing advancements in both medical therapies and devices. Of these possibilities, we propose that phenotypic variation of aortic stenosis has the most significant impact on post-TAVI clinical outcomes. Such variability in phenotypes is often due to a complex interplay between underlying comorbidities and environmental and inherent patient risk factors. However, there is growing evidence to suggest that patient genetics may also play a role in aortic stenosis pathology. As such, we propose that the selection and management of TAVI patients should emphasize a precision medicine approach.
Nutritional State, Immunological and Biochemical Parameters, and Mortality in the ICU: An Analytical Study
Authors: Díaz Chavarro Blanca Cecilia , Romero-Saldaña Manuel , Assis Reveiz Jorge Karim , Molina-Recio Guillermo
Published: June 2023

Intensive care unit (ICU) hospitalization involves critically ill patients with multiple diseases and possible complications, including malnutrition, which increases hospital stay and mortality. Therefore, identifying the patient’s prior nutritional state, following up during hospitalization, and implementing early intervention positively affect patient’s vital situations at discharge. The objective of this study is to determine the nutritional state of patients admitted to an ICU in Cali (Colombia) in 2019 and its association with immunological and biochemical parameters and mortality observed during hospitalization. This was an observational, analytical, and retrospective study of patients admitted to an ICU in a clinic in Cali (Colombia) from 1 January to 31 March 2019. The association between their nutritional state and outcome variables such as hospital stay, immunological and biochemical function, and mortality was analyzed. Logistic regression was used to predict patients’ vital status at hospital discharge. In terms of the nutritional level, low weight was observed in 17.5% patients, and overweight/obesity was observed in 53.5% of the population. Nutritional state was associated with leukocytosis. The patients with lymphocytosis had longer hospital stays than those with normal lymphocyte ranges. Age, blood leukocytes, and creatinine and potassium levels increased the risk of mortality. Lymphocyte values have been used as predictors of severity and hospitalization time. The scientific literature has also evidenced a higher leukocyte count in people with obesity, and such leukocytosis is associated with the risk of mortality. The results of blood and laboratory tests determining kidney function and blood electrolytes allow for the prediction of mortality risk in critically ill patients.
Prevalence and Characteristics of Itch and Pain in Patients Suffering from Chronic Hand Eczema
Authors: Zalewski Adam , Krajewski Piotr K. , Szepietowski Jacek C.
Published: June 2023

Background: Hand eczema (HE) is a frequent chronic inflammatory dermatosis. Itch and pain are considered two of the most common and burdensome symptoms of the disease. Yet, the data related to these symptoms are still limited. The aim of this study was to evaluate characteristics of itch and pain in adults suffering from HE. Methods: The study group comprised 100 adult HE patients. An original questionnaire designed by the authors was used to survey the patients. It included questions regarding demographic characteristics such as the duration of the disease, exacerbation count, past diagnostics and treatment, as well as atopic predispositions. Additionally, the itch and pain intensity (numerical rating scale—NRS) during ‘3 days prior to the study’ and the ‘entire disease’ period was implemented. The clinical assessment of the disease severity was performed according to two specific measurement instruments: Investigator Global Assessment for Chronic Hand Eczema (IGA-CHE) scale and Hand Eczema Severity Index (HECSI). To assess patient quality of life (QoL), the DLQI tool was used and to determine the level of stigmatization and for its impact on patients’ life the 6-Item Stigmatization Scale (6-ISS) was employed. Results: Within the period of 3 days prior to the examination, itch was reported by 81.0% of patients (n = 81), whereas 53.0% (n = 53) of them experienced pain. Both symptoms were reported more frequently in females (itch: p = 0.022; pain: p = 0.033). When sexes were compared, females reached higher scores in both IGA-CHE and HECSI. Itch and pain intensity correlated positively with disease severity. The intensity of itch and pain significantly influences HE patients’ QoL. A positive correlation between the 6-ISS score and the intensity of itch in the ‘last 3 days’ period was revealed (r = 0.221; p = 0.027). Conclusions: Itch and pain are common symptoms in HE patients, significantly contributing to the feeling of stigmatization. Providing characteristics of itch and pain may improve HE management. Symptom-decreasing treatment would definitely have a positive influence on patients’ well-being.
On the Stability of Rubble Mound Structures under Oblique Wave Attack
Authors: Bali Meysam , Etemad-Shahidi Amir , van Gent Marcel R. A.
Published: June 2023

Slope stability formulae for rubble mound structures are usually developed for head-on conditions. Often, the effects of oblique waves are neglected, mainly because it is assumed that for oblique wave attack, the reduction in damage compared to perpendicular wave attack is insignificant. When the incident waves are oblique, the required armour size can be reduced compared to the perpendicular wave attack case. Therefore, it is important to consider the wave obliquity influence on slope stability formulae as a reduction factor. One of the most recent formulae for estimating the stability of rock-armoured slopes, referred to as Etemad-Shahidi et al. (2020), was proposed for perpendicular wave attack. The aim of this study is to develop a suitable wave obliquity reduction factor for the above-mentioned stability formula. To achieve this, first, laboratory experiment datasets from existing reliable studies were selected and analysed. Then, previously suggested reduction factors were evaluated and a suitable reduction factor for the mentioned stability formula were suggested. The suggested reduction factor includes the effect of wave obliquity and directional spreading explicitly. It is shown that the stability prediction is improved by using the wave obliquity reduction factor.
Experimental Research on the Stiffness Step between the Main Hull and Superstructure of Cruise Ships
Authors: Pei Zhiyong , Yang Bin , Liu Guangwu , Wu Weiguo
Published: June 2023

The demand for larger passenger capacity and more entertainment facilities has led to the rapid growth of the cruise tourism market. The superstructure of cruise ships is designed to be plumper, with numerous decks and complex structural forms. To control the weight and the center of gravity, the bending stiffness of the superstructure is always designed to be weaker than that of the main hull, resulting in a stiffness step. Currently, there is no satisfactory method to accurately estimate the influence of the stiffness step between the main hull and superstructure on the structural response of cruise ships. In the present research, an experimental analysis is conducted to investigate the stiffness step between the main hull and superstructure of a typical cruise ship. By comparing the longitudinal stress distribution characteristics with and without the stiffness step with the theoretical results, the influence of the stiffness step on the longitudinal strength is investigated. Furthermore, the maximum stress and the bending efficiency of the superstructure are also discussed. The present research is of reference significance for the structural safety and reliability design of cruise ships.
Research on Chinese Semantic Named Entity Recognition in Marine Engine Room Systems Based on BERT
Authors: Shen Henglong , Cao Hui , Sun Guangxi , Chen Dong
Published: June 2023

With the development of intelligentization in maritime vessels, the pursuit of an organized and scalable knowledge storage approach for marine engine room systems has become one of the current research hotspots. This study addressed the foundational named entity recognition (NER) task in constructing a knowledge graph for marine engine rooms. It proposed an entity recognition algorithm for Chinese semantics in marine engine rooms that integrates language models. Firstly, the bidirectional encoder representation from transformers (BERT) language model is used to extract text features and obtain word-level granularity vector matrices. Secondly, the trained word embeddings are fed into a bidirectional long short-term memory network (BiLSTM) to extract contextual information. It considers the surrounding words and their sequential relationships, enabling a better understanding of the context. Additionally, the conditional random field (CRF) model was used to extract the globally optimal sequence of named entities in the marine engine room semantic. The CRF model considered the dependencies between adjacent entities that ensured a coherent and consistent final result for entity recognition in marine engine room semantics. The experiment results demonstrate that the proposed algorithm achieves superior F1 scores for all three entity types. Compared with BERT, the overall precision, recall, and F1 score of the entity recognition are improved by 1.36%, 1.41%, and 1.38%, respectively. Future research will be carried out on named entity recognition of a small sample set to provide basic support for more efficient entity relationship extraction and construction of a marine engine room knowledge graph.
HL7-FHIR-Based ContSys Formal Ontology for Enabling Continuity of Care Data Interoperability
Authors: Das Subhashis , Hussey Pamela
Published: June 2023

The rapid advancement of digital technologies and recent global pandemic-like scenarios have pressed our society to reform and adapt health and social care toward personalizing the home care setting. This transformation assists in avoiding treatment in crowded secondary health care facilities and improves the experience and impact on both healthcare professionals and service users alike. The interoperability challenge through standards-based roadmaps is the lynchpin toward enabling the efficient interconnection between health and social care services. Hence, facilitating safe and trustworthy data workflow from one healthcare system to another is a crucial aspect of the communication process. In this paper, we showcase a methodology as to how we can extract, transform and load data in a semi-automated process using a common semantic standardized data model (CSSDM) to generate a personalized healthcare knowledge graph (KG). CSSDM is based on a formal ontology of ISO 13940:2015 ContSys for conceptual grounding and FHIR-based specification to accommodate structural attributes to generate KG. The goal of CSSDM is to offer an alternative pathway to discuss interoperability by supporting a unique collaboration between a company creating a health information system and a cloud-enabled health service. The resulting pathway of communication provides access to multiple stakeholders for sharing high-quality data and information.
Smile Design and Treatment Planning—Conventional versus Digital—A Pilot Study
Authors: Chisnoiu Andrea Maria , Staicu Andreea Cristina , Kui Andreea , Chisnoiu Radu Marcel , Iacob Simona , Fluerașu Mirela , Buduru Smaranda
Published: June 2023

Introduction: Several methods are currently available for providing a preview of the prosthodontic treatment, including computer simulations, 3D models, wax-ups, and mock-ups. The aim of this study is to compare the aesthetic aspects and assessment of conventional versus digital prefigurative methods. Methods: The study included 5 patients and 3 observers, for each of whom a wax-up was made in both the conventional and digital techniques. The analog method, which implied a mock-up molding with a silicone matrix of the wax-up, was compared to a digital workflow, which consisted of a mock-up milling from a digital design. The patient’s clinical mock-ups were recorded with digital photographs and assessed for nine different criteria by three observers. Results: The analysis has shown a balanced assessment of the aesthetic criteria without any significant difference between the analog and digital prefigurative methods. Conclusions: Between the two wax-ups (conventional and digital), there were some variations in smile and dental criteria; however, the obtained data were very similar. When it comes to the smile criteria, the general average grades of the mock-ups conducted using the conventional method are slightly higher than the ones using the digital technique.
Differential Impact of Fintech and GDP on Bank Performance: Global Evidence
Authors: Yoon Soon , Lee Hongbok , Oh Ingyu
Published: June 2023

Using the World Bank Global Findex Database for 91 countries in 2014, 2017, and 2021, we examine whether fintech levels influence bank performance and whether fintech’s interaction with GDP per capita causes differential effects on bank performance globally. Since fintech levels were already very high for rich countries when the World Bank started providing fintech development statistics in 2014, we estimate AbFintech by regressing fintech levels on GDP per capita by year. AbFintech is the difference between the fintech level and its fitted values. Then, using multiple regression analyses, we investigate the impact of AbFintech on bank performance worldwide, focusing on the differential effects of AbFintech and GDP levels on bank performance. We find AbFintech significantly increases bank performance, primarily in less developed countries. Specifically, AbFintech increases banks’ ROA in the least developed countries and net interest margin in 75th percentile countries. Also, AbFintech decreases the cost-to-income ratio in 75th percentile countries, while it increases the ratio in the most developed countries. The resulting policy implication is that banks in less developed countries benefit most from investing in fintech innovation since they can provide a broader customer base, including formerly unbanked or underbanked customers, with more convenient services at lower costs.
Quantifying Dieback in a Vulnerable Population of Eucalyptus macrorhyncha Using Remote Sensing
Authors: Fitzgerald Donna L. , Peters Stefan , Guerin Gregory R. , McGrath Andrew , Keppel Gunnar
Published: June 2023

A disjunct population of red stringybark (Eucalyptus macrorhyncha) trees in South Australia is experiencing increasing amounts of dieback. Because the population is considered vulnerable to extinction, we investigated spatiotemporal vegetation changes, quantified the extent of dieback, and determined how topography influences dieback using aerial and satellite imagery. Classification of vegetation health status using hyperspectral aerial imagery indicated that 37% (accuracy = 0.87 Kappa) of the population was unhealthy and potentially experiencing dieback. When correlating this classification with a digital terrain model (DTM), the aspect and amount of solar radiation had the strongest relationship with the presence of unhealthy vegetation. PlanetScope satellite-derived, and spectral index-based analysis indicated that 7% of the red stringybark population experienced negative vegetation health changes during a five-year period (2017–2022), with positive vegetation health changes (9.5%) noted on pole-facing slopes. Therefore, our integrated remote sensing approach documented the extent and spatiotemporal dynamics of dieback, suggesting it could be applied in other studies. Topographical aspects exposed to high-solar radiation were particularly vulnerable to dieback, and pole-facing aspects demonstrated some recovery between droughts. The influence of topography and maps of vegetation health can be used to guide future management and restoration of the population.
An Overview of Cefiderocol’s Therapeutic Potential and Underlying Resistance Mechanisms
Authors: Domingues Sara , Lima Tiago , Saavedra Maria José , Da Silva Gabriela Jorge
Published: June 2023

Antimicrobial resistance continues to increase globally and treatment of difficult-to-treat (DTT) infections, mostly associated with carbapenem-resistant (CR) Pseudomonas aeruginosa, CR Acinetobacter baumannii, and CR- and third-generation-cephalosporins-resistant Enterobacterales remains a challenge for the clinician. The recent approval of cefiderocol has broaden the armamentarium for the treatment of patients with DTT infections. Cefiderocol is a siderophore cephalosporin that has shown excellent antibacterial activity, in part due to its innovative way of cell permeation. It is relatively stable compared to most commonly found carbapenamases. However, some resistant mechanisms to cefiderocol have already been identified and reduced susceptibility has developed during patient treatment, highlighting that the clinical use of cefiderocol must be rational. In this review, we summarize the current available treatments against the former resistant bacteria, and we revise and discuss the mechanism of action of cefiderocol, underlying the biological function of siderophores, the therapeutic potential of cefiderocol, and the mechanisms of resistance reported so far.
Combined Effect of Fluid Cavitation and Inertia on the Pressure Buildup of Parallel Textured Surfaces
Authors: Ma Xuezhong
Published: June 2023

A mathematical model is developed to investigate the combined effect of fluid cavitation and inertia on the fluid pressure buildup of parallel textured surfaces. The fluid cavitation is analyzed using the Rayleigh–Plesset model, and the fluid inertia is analyzed with an averaged method. The finite element method and Newton-downhill method are employed to solve the governing equations. The numerical model is validated by comparing the experimental and numerical results, and the combined effect of fluid cavitation and inertia on the fluid pressure buildup is analyzed and discussed. The research indicates that the cavitation weakens the fluid inertia effect on the pressure distribution at the inlet area of textures. The fluid inertia greatly enhances the hydrodynamic effect and effectively limits the excessive extension of the low-pressure zone caused by cavitation. The fluid cavitation and inertia, especially their interaction, significantly affect the fluid pressure buildup and generate a net load-carrying capacity (LCC). The numerical model with the fluid inertia and cavitation is more time saving than the commercial CFD tools in solutions, which gives a novel and optional HD foundation for developing a more efficient and accurate THD or TEHD model by numerical programming.
Modeling Asymmetric Volatility: A News Impact Curve Approach
Authors: Rakshit Debopam , Paul Ranjit , Yeasin Md , Emam Walid , Tashkandy Yusra , Chesneau Christophe
Published: June 2023

Seasonal production, weather abnormalities, and high perishability introduce a high degree of volatility to potato prices. Price volatility is said to be asymmetric when positive and negative shocks of the same magnitude affect it in a dissimilar way. GARCH is a symmetric model, and it cannot capture asymmetric price volatility. EGARCH, APARCH, and GJR-GARCH models are popularly used to capture asymmetric price volatility. In this paper, an attempt is made to model the price volatility of the weekly wholesale modal price of potatoes for the Agra, Ahmedabad, Bengaluru, Delhi, Kolkata, and Mumbai markets using the above-mentioned models. The News Impact Curves (NICs) are derived from the fitted models, which confirmed the presence of asymmetry in the price volatility. To this end, NICs are used to describe the degree of asymmetry in volatility present in different markets.
A Protocol for Solutions to DP-Complete Problems through Tissue Membrane Systems
Authors: Orellana-Martín David , Ramírez-de-Arellano Antonio , Andreu-Guzmán José Antonio , Romero-Jiménez Álvaro , Pérez-Jiménez Mario J.
Published: June 2023

Considering a class R comprising recognizer membrane systems with the capability of providing polynomial-time and uniform solutions for NP-complete problems (referred to as a “presumably efficient” class), the corresponding polynomial-time complexity class PMCR encompasses both the NP and co-NP classes. Specifically, when R represents the class of recognizer presumably efficient cell-like P systems that incorporate object evolution rules, communication rules, and dissolution rules, PMCR includes both the DP and co-DP classes. Here, DP signifies the class of languages that can be expressed as the difference between any two languages in NP (it is worth noting that NP ⊆ DP and co-NP⊆co-DP). As DP-complete problems are believed to be more complex than NP-complete problems, they serve as promising candidates for studying the P vs. NP problem. This outcome has previously been established within the realm of recognizer P systems with active membranes. In this paper, we extend this result to encompass any class R of presumably efficient recognizer tissue-like membrane systems by presenting a detailed protocol for transforming solutions of NP-complete problems into solutions of DP-complete problems.
A Combinatorial Optimization Approach for Air Cargo Palletization and Aircraft Loading
Authors: Zhao Xiangling , Dong Yun , Zuo Lei
Published: June 2023

The current air cargo loading plan handles the Air Cargo Palletization Problem (ACPP) and the Aircraft Weight and Balance Problem (WBP) separately, which has an impact on the optimization of the payload and the aircraft’s center of gravity (CG). Thanks to improvements in computer processing power, the joint combinatorial optimization of ACPP and WBP is now feasible. Three integer linear programming models are proposed: a Bi-objective Optimization Model (BOM), a Combinatorial Optimization Model (COM), and an Improved Combinatorial Optimization Model (IOM). The objectives of the models are the maximum loading capacity and the lowest CG deviation from a specified target CG. The models also consider a wide range of restrictions in the actual packing and stowage procedures, such as volume, weight, loading position, aircraft balance, and other aspects of aircraft and unit load devices. Four scenarios with various conditional metrics for three models are solved for the B777F aircraft using Gurobi. The results of the computations demonstrate that the BOM has the fastest solution speed, but the CG deviation is the largest, and in several cases the CG deviation results are unacceptable. The COM has the longest solution time, which is difficult to tolerate in practice. Despite taking a little longer to solve computationally than the BOM, the IOM offers the best optimization solution.
Medial Meniscus Posterior Root Tear: How Far Have We Come and What Remains?
Authors: Moon Hyun-Soo , Choi Chong-Hyuk , Jung Min , Chung Kwangho , Jung Se-Han , Kim Yun-Hyeok , Kim Sung-Hwan
Published: June 2023

Medial meniscus posterior root tears (MMRTs), defined as tears or avulsions that occur within 1 cm of the tibial attachment of the medial meniscus posterior root, lead to biomechanically detrimental knee conditions by creating a functionally meniscal-deficient status. Given their biomechanical significance, MMRTs have recently been gaining increasing interest. Accordingly, numerous studies have been conducted on the anatomy, biomechanics, clinical features, diagnosis, and treatment of MMRTs, and extensive knowledge has been accumulated. Although a consensus has not yet been reached on several issues, such as surgical indications, surgical techniques, and rehabilitation protocols, this article aimed to comprehensively review the current knowledge on MMRTs and to introduce the author’s treatment strategies.
Unveiling the Enigmatic Adenoids and Tonsils: Exploring Immunology, Physiology, Microbiome Dynamics, and the Transformative Power of Surgery
Authors: Samara Pinelopi , Athanasopoulos Michael , Athanasopoulos Ioannis
Published: June 2023

Within the intricate realm of the mucosal immune system resides a captivating duo: the adenoids (or pharyngeal tonsils) and the tonsils (including palatine, tubal, and lingual variations), which harmoniously form the Waldeyer’s ring. As they are strategically positioned at the crossroads of the respiratory and gastrointestinal systems, these exceptional structures fulfill a vital purpose. They function as formidable “gatekeepers” by screening microorganisms—both bacteria and viruses—with the mission to vanquish local pathogens via antibody production. However, under specific circumstances, their function can take an unsettling turn, inadvertently transforming them into reservoirs for pathogen incubation. In this review, we embark on a fascinating journey to illuminate the distinctive role of these entities, focusing on the local immune system inside their tissues. We delve into their behavior during inflammation processes, meticulously scrutinize the indications for surgical intervention, and investigate the metamorphosis of their microbiota in healthy and diseased states. We explore the alterations that occur prior to and following procedures like adenoidectomy, tonsillectomy, or their combined counterparts, particularly in pediatric patients. By comprehending a wealth of data, we may unlock the key to the enhanced management of patients with otorhinolaryngological disorders. Empowered with this knowledge, we can embrace improved therapeutic approaches and targeted interventions/surgeries guided by evidence-based guidelines and indications.
Synthesis of TiO2-Cu2+/CuI Nanocomposites and Evaluation of Antifungal and Cytotoxic Activity
Authors: Hernandez Rafael , Jimenez-Chávez Arturo , De Vizcaya Andrea , Lozano-Alvarez Juan Antonio , Esquivel Karen , Medina-Ramírez Iliana E.
Published: June 2023

Fungal infections have become a significant public health concern due to their increasing recurrence and harmful effects on plants, animals, and humans. Opportunistic pathogens (among others from the genera Candida and Aspergillus) can be present in indoor air, becoming a risk for people with suppressed immune systems. Engineered nanomaterials are novel alternatives to traditional antifungal therapy. In this work, copper(I) iodide (CuI) and a copper-doped titanium dioxide—copper(I) iodide (TiO2-Cu2+/CuI) composite nanomaterials (NMs)—were synthesized and tested as antifungal agents. The materials were synthesized using sol-gel (TiO2-Cu2+) and co-precipitation (CuI) techniques. The resulting colloids were evaluated as antifungal agents against Candida parapsilosis and Aspergillus niger strains. The NMs were characterized by XRD, HRTEM, AFM, and DLS to evaluate their physicochemical properties. The NMs present a high size dispersion and different geometrical shapes of agglomerates. The antifungal capacity of the NMs by the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) was below 15 µg/mL against Candida parapsilosis and below 600 µg/mL against Aspergillus niger for both NMs. Holotomography microscopy showed that the NMs could penetrate cell membranes causing cell death through its rupture and reactive oxygen species (ROS) production. Cytotoxicity tests showed that NMs could be safe to use at low concentrations. The synthesized nanomaterials could be potential antifungal agents for biomedical or environmental applications.
Glucose Transporter-2 Regulation of Male versus Female Hypothalamic Astrocyte MAPK Expression and Activation: Impact of Glucose
Authors: Pasula Madhu Babu , Roy Sagor C. , Bheemanapally Khaggeswar , Sylvester Paul W. , Briski Karen P.
Published: June 2023

The plasma membrane glucose transporter (GLUT)-2 is unique among GLUT family proteins in that it also functions as a glucose sensor. GLUT2 imposes sex-dimorphic control of hypothalamic astrocyte glucose storage and catabolism by unknown mechanisms. Mitogen-activated protein kinase (MAPK) signaling cascades operate within stress-sensitive signal transduction pathways. The present study employed an established primary astrocyte culture model and gene knockdown tools to investigate whether one or more of the three primary MAP kinase families are regulated by GLUT2. GLUT2 gene knockdown caused opposing adjustments in total ERK1/2 proteins in glucose-supplied male versus female astrocytes, augmenting or reducing the mean phosphorylated/total protein ratio for 44 and 42 kDa variants in these sexes. Glucose deprivation amplified this ratio for both ERK1/2 variants, albeit by a larger magnitude in males; GLUT2 siRNA exacerbated this stimulatory response in males only. Phosphorylated/total p38 MAPK protein ratios were up-regulated by GLUT2 knockdown in male, but not female astrocytes. Glucose-deprived astrocytes exhibited no change (male) or reduction (female) in this ratio after GLUT2 gene silencing. GLUT2 siRNA increased the phosphorylated/total protein ratio for 54 and 46 kDa SAPK/JNK proteins in each sex when glucose was present. However, glucose withdrawal suppressed (male) or amplified (female) these ratios, while GLUT2 knockdown attenuated these inverse responses. The results show that GLUT2 inhibits ERK1/2, p38, and SAPK/JNK MAPK activity in male astrocytes, but differentially stimulates and inhibits activity of these signaling pathways in female hypothalamic astrocytes. Glucoprivation induces divergent adjustments in astrocyte p38 MAPK and SAPK/JNK activities. The findings demonstrate a stimulatory role for GLUT2 in p38 MAPK activation in glucose-starved female astrocytes, but it can act as either an inhibitor or inducer of SAPK/JNK activation in glucose-deprived male versus female glial cells, respectively.
Pre-Season Nutritional Intake and Prevalence of Low Energy Availability in NCAA Division III Collegiate Swimmers
Authors: Klein Dylan J. , McClain Patrick , Montemorano Victoria , Santacroce Alaina
Published: June 2023

There is limited information regarding the dietary habits and energy availability (EA) of collegiate athletes. Therefore, the purpose of the present study is to assess the nutrient intakes, dietary habits, and prevalence of low EA (<30 kcals/kg FFM) in a group of National Collegiate Athletic Association (NCAA) Division III male and female swimmers. Energy and nutrient intake, body composition, and exercise energy expenditure was assessed in 30 (n = 15 males, n = 15 females) NCAA Division III swimmers during pre-season using three-day diet and seven-day activity records alongside multi-frequency, bioelectric impedance analysis. A validated screening tool was used to assess for low EA in the female swimmers. Mean EA in male and female athletes was 32.7 ± 12 and 34.9 ± 13.7 kcals/kg FFM, respectively, and was not significantly different between the sexes (p = 0.65). Twenty percent of swimmers (n = 3 males, n = 3 females) presented with optimal EA, 37% (n = 5 males, n = 6 females) presented with sub-optimal EA, and 43% (n = 7 males, n = 6 females) presented with low EA. Swimmers who presented with a low EA consumed significantly less calories, carbohydrates, and proteins than non-low EA swimmers (p < 0.02). The validated screening tool failed to classify 50% of female swimmers who presented with low EA. Only eight athletes achieved the USDA MyPlate recommendation for fruits, whereas three athletes achieved the recommendation for vegetables, with no differences between the sexes (p > 0.05). The present findings show that there was a high prevalence of low EA during the pre-season among male and female collegiate swimmers that was not fully captured using a validated screening tool for females. Low EA occurred alongside lower intakes of calories, carbohydrates, and proteins, and the majority of swimmers did not meet the United States Department of Agriculture recommendations for fruit and vegetable intake. These data stress the need for improved dietary intakes in NCAA Division III collegiate swimmers.
Association between the Preoperative Dietary Antioxidant Index and Postoperative Quality of Life in Patients with Esophageal Squamous Cell Carcinoma: A Prospective Study Based on the TTD Model
Authors: Zhang Juwei , Zhou Jinsong , Huang Yue , Lin Zheng , Zhang Suhong , Qiu Minglian , Xiang Zhisheng , Hu Zhijian
Published: June 2023

Objective: Dietary antioxidants are associated with risk of death in cancer patients, and they were used to evaluate the prognosis of cancer patients. Dietary antioxidant index (DAI) can be used to evaluate dietary antioxidant content comprehensively; this study aimed to investigate the effect of preoperative DAI on health-related quality of life in patients with esophageal cell squamous carcinoma (ESCC). Methods: Data on dietary intakes were collected using a validated food-frequency questionnaire (FFQ). DAI was calculated for all study participants based on FFQ data of each participant. The study involved conducting several follow-up activities with patients diagnosed with ESCC to evaluate their quality of life. The approach employed in the study was to conduct a telephone interview. The EORTC Quality of Life Questionnaire-Core Questionnaire (EORTC QLQ-C30, version 3.0) and the Esophageal Cancer Module (EORTC QLQ-OES18) were used to collect data on the quality of life of the patients; all patients completed the full follow-up. Results: This prospective study was performed on 376 participants who were recruited from Fujian Cancer Hospital and First Hospital of Fujian Medical University. They all were diagnosed with ESCC. The results indicated that the time to deterioration of global health status (p = 0.043), cognitive functioning (p = 0.031), dry mouth (p = 0.019), and speech problems (p = 0.031) significantly delay in the high DAI group. Univariate and multivariate Cox regression analysis showed that global health status (HR = 0.718, 95% CI: 0.532–0.969), cognitive functioning (HR = 0.641, 95% CI: 0.450–0.913), dry mouth (HR = 0.637, 95% CI: 0.445–0.911), and speech problems (HR = 0.651, 95% CI: 0.449–0.945) were improved in the high DAI group. Conclusions: Prognostic value of preoperative DAI was significant for patients with ESCC who undergo surgical intervention. Its level was positively correlated with the postoperative quality of life of patients, which can delay and improve the occurrence of postoperative physical function and symptom deterioration.
Dietary Calcium Intake and Osteoporosis Risk in Arab Adults
Authors: Al-Daghri Nasser M. , Hussain Syed Danish , Alnaami Abdullah M. , Aljohani Naji , Sabico Shaun
Published: June 2023

Osteoporosis is a major public health concern in Saudi Arabia’s aging population. There is particularly limited information on how diet affects bone loss in this ethnic group. The purpose of this study was to examine the association between dietary calcium (Ca) intake and osteoporosis risk in Saudi adults. A total of 1950 patients (416 males and 1534 females) with known risk factors for osteoporosis participated in this cross-sectional study. A short questionnaire (CaQ) was used to assess dietary Ca intakes in patients attending tertiary hospitals in Riyadh City. The prevalence of osteoporosis was 21.3% and was more common in females (93.5%). Patients with osteoporosis were older (p < 0.001) and had lower BMI (p < 0.001). Results showed that the overall mean Ca intake was only 445.1 mg/day (recommended dietary intake of 1300 mg/day). Tea intake (OR = 0.8 95%CI: 0.7–1.0; p = 0.02) and consumption of fish and eggs (OR = 0.9 95%CI: 0.8–1.0; p = 0.01) were significantly associated with a lower risk of osteoporosis. Furthermore, consumption of biscuits, cake and bread slices were significantly associated with higher incidence of osteoporosis (OR = 1.3 95%CI: 1.0–1.5; p = 0.02). In conclusion, extremely low dietary Ca intake was observed among Saudi adults already at risk of osteoporosis. A balanced diet including high amount of Ca, vitamin D and omega-3 fatty acids accompanied by limiting consumption of foods high in saturated fats and glycemic index may be helpful in reducing osteoporosis risk in the Saudi adult population.
Role of Gut Microbiota in Overweight Susceptibility in an Adult Population in Italy
Authors: Politi Cristina , Mobrici Marco , Parlongo Rosa Maria , Spoto Belinda , Tripepi Giovanni , Pizzini Patrizia , Cutrupi Sebastiano , Franco Daniele , Tino Renato , Farruggio Giuseppe , Failla Chiara , Marino Flavia , Pioggia Giovanni , Testa Alessandra
Published: June 2023

Although the gut microbiota is known to affect body weight, its relationship with overweight/obesity is unclear. Our aim was to characterize microbiota composition in a cohort from the southernmost area of Italy. We investigated whether an altered gut microbiota could play an etiological role in the pathogenesis of overweight/obesity. A total of 163 healthy adults were enrolled. Microbiome analysis was performed via 16S rRNA gene sequencing. We found significant phylum variations between overweight (N = 88) and normal-weight (N = 75) subjects. Bacteroidetes and Proteobacteria were higher in overweight participants (p = 0.004; p = 0.03), and Firmicutes and Verrucomicrobia were lower (p = 0.02; p = 0.008) compared to normal-weight participants. Additionally, Akkermansia and Bifidobacterium (genus level) were significantly lower in the overweight group, as well as Akkermansia muciniphila at the species level. The Firmicutes/Bacteroidetes ratio (F/B ratio), an index of dysbiosis, was found to be inversely associated with BMI in linear and logistic regression models (p = 0.001; p = 0.005). The association remained statistically significant after adjustment for potential confounders. This cross-sectional study contributes to defining the gut microbiota composition in an adult population living in southern Italy. It confirms the relationship between overweight susceptibility and the dysbiosis status, highlighting the possible etiological role of the F/B ratio in disease susceptibility.
Molecular Mimicry Mapping in Streptococcus pneumoniae: Cues for Autoimmune Disorders and Implications for Immune Defense Activation
Authors: Mashraqi Mutaib M. , Alzamami Ahmad , Alturki Norah A. , Alshamrani Saleh , Alshahrani Mousa M. , Almasoudi Hassan H. , Basharat Zarrin
Published: June 2023

Streptococcus pneumoniae contributes to a range of infections, including meningitis, pneumonia, otitis media, and sepsis. Infections by this bacterium have been associated with the phenomenon of molecular mimicry, which, in turn, may contribute to the induction of autoimmunity. In this study, we utilized a bioinformatics approach to investigate the potential for S. pneumoniae to incite autoimmunity via molecular mimicry. We identified 13 S. pneumoniae proteins that have significant sequence similarity to human proteins, with 11 of them linked to autoimmune disorders such as psoriasis, rheumatoid arthritis, and diabetes. Using in silico tools, we predicted the sequence as well as the structural homology among these proteins. Database mining was conducted to establish links between these proteins and autoimmune disorders. The antigenic, non-allergenic, and immunogenic sequence mimics were employed to design and validate an immune response via vaccine construct design. Mimic-based vaccine construct can prove effective for immunization against the S. pneumoniae infections. Immune response simulation and binding affinity was assessed through the docking of construct C8 to human leukocyte antigen (HLA) molecules and TLR4 receptor, with promising results. Additionally, these mimics were mapped as conserved regions on their respective proteins, suggesting their functional importance in S. pneumoniae pathogenesis. This study highlights the potential for S. pneumoniae to trigger autoimmunity via molecular mimicry and the possibility of vaccine design using these mimics for triggering defense response.
Numerical Simulation of High-Power Optical Amplifiers at 2.3 µm Based on a Special Multicore Fiber
Authors: Anashkina Elena A. , Andrianov Alexey V. , Litvak Alexander G.
Published: June 2023

The development of high-power laser sources at 2.3 µm is highly demanded for remote sensing and other applications. However, this wavelength is poorly covered by present-day lasers. To obtain 100 W class high-power radiation at 2.3 μm, we propose to use simultaneously cascade laser amplification at 2 and 2.3 µm with a commercially available diode pump at 793 nm and stimulated Raman scattering between the amplified signal waves in a special zinc–tellurite multicore fiber with ten trivalent-thulium-ion-doped cores arranged in a ring. We demonstrate numerically that the use of an out-of-phase supermode (with spatial phases differing by π in neighboring cores) can provide up to 50% efficiency conversion from the 793 nm pump to the 2.3 µm wave.
Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in Ilex paraguariensis Roots
Authors: Avico Edgardo H. , Acevedo Raúl M. , Duarte María J. , Rodrigues Salvador Acácio , Nunes-Nesi Adriano , Ruiz Oscar A. , Sansberro Pedro A.
Published: June 2023

The appearance of water stress episodes triggers leaf abscission and decreases Ilex paraguariensis yield. To explore the mechanisms that allow it to overcome dehydration, we investigated how the root gene expression varied between water-stressed and non-stressed plants and how the modulation of gene expression was linked to metabolite composition and physiological status. After water deprivation, 5160 differentially expressed transcripts were obtained through RNA-seq. The functional enrichment of induced transcripts revealed significant transcriptional remodelling of stress-related perception, signalling, transcription, and metabolism. Simultaneously, the induction of the enzyme 9-cis-expoxycarotenoid dioxygenase (NCED) transcripts reflected the central role of the hormone abscisic acid in this response. Consequently, the total content of amino acids and soluble sugars increased, and that of starch decreased. Likewise, osmotic adjustment and radical growth were significantly promoted to preserve cell membranes and water uptake. This study provides a valuable resource for future research to understand the molecular adaptation of I. paraguariensis plants under drought conditions and facilitates the exploration of drought-tolerant candidate genes.
A New Repellent for Redbay Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae), Primary Vector of the Mycopathogen That Causes Laurel Wilt
Authors: Cloonan Kevin R. , Montgomery Wayne S. , Narvaez Teresa I. , Kendra Paul E.
Published: June 2023

The redbay ambrosia beetle, Xyleborus glabratus, was detected in Georgia, USA, in 2002 and has since spread to 11 additional states. This wood-boring weevil carries a symbiotic fungus, Harringtonia lauricola, that causes laurel wilt, a lethal disease of trees in the Lauraceae family. Native ambrosia beetles that breed in infected trees can acquire H. lauricola and contribute to the spread of laurel wilt. Since 2002, laurel wilt has devastated native Persea species in coastal forests and has killed an estimated 200,000 avocado trees in Florida. Since laurel wilt is difficult to manage once it has entered a susceptible agrosystem, this study evaluated piperitone as a candidate repellent to deter attacks by X. glabratus and other ambrosia beetles. Additionally, piperitone was compared to the known repellent verbenone as a potential cost-effective alternative. The repellent efficacy was determined by comparing captures in traps baited with commercial beetle lures containing α-copaene versus captures in traps baited with lures plus a repellent. In parallel 10-week field tests, the addition of piperitone reduced the captures of X. glabratus in α-copaene-baited traps by 90%; however, there was no significant reduction in the captures of native ambrosia beetles in ethanol-baited traps. In two replicate 10-week comparative tests, piperitone and verbenone both reduced X. glabratus captures by 68–90%, with longevity over the full 10 weeks. This study identifies piperitone as a new X. glabratus repellent with potential for pest management.
Overexpression of Rice Histone H1 Gene Reduces Tolerance to Cold and Heat Stress
Authors: Wan Jiale , Zhang Jia , Zan Xiaofei , Zhu Jiali , Chen Hao , Li Xiaohong , Zhou Zhanmei , Gao Xiaoling , Chen Rongjun , Huang Zhengjian , Xu Zhengjun , Li Lihua
Published: June 2023

Temperature stresses, including low- and high-temperature stresses, are the main abiotic stresses affecting rice yield. Due to global climate change, the impact of temperature pressure on rice yield is gradually increasing, which is also a major concern for researchers. In this study, an H1 histone in Oryza sativa (OsHis1.1, LOC_Os04g18090) was cloned, and its role in rice’s response to temperature stresses was functionally characterized. The GUS staining analysis of OsHis1.1 promoter-GUS transgenic rice showed that OsHis1.1 was widely expressed in various rice tissues. Transient expression demonstrated that OsHis1.1 was localized in the nucleus. The overexpression of OsHis1.1 reduces the tolerance to temperature stress in rice by inhibiting the expression of genes that are responsive to heat and cold stress. Under stress conditions, the POD activity and chlorophyll and proline contents of OsHis1.1-overexpression rice lines were significantly lower than those of the wild type, while the malondialdehyde content was higher than that of the wild type. Compared with Nip, OsHis1.1-overexpression rice suffered more serious oxidative stress and cell damage under temperature stress. Furthermore, OsHis1.1-overexpression rice showed changes in agronomic traits.