bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen
Authors: Sotnikov Dmitriy V. , Byzova Nadezhda A. , Zherdev Anatoly V. , Xu Youchun , Dzantiev Boris B.
Published: July 2023

Reliable detection of specific antibodies against pathogens by lateral flow immunoassay (LFIA) greatly depends on the composition of the detectable complex and the order of its assembly. We compared three LFIA formats for revealing anti-SARS-CoV-2 antibodies in sera with the following detected complexes in the analytical zone of the strip: antigen–antibodies–labeled immunoglobulin-binding protein (Scheme A); antigen–antibodies–labeled antigen (Scheme B); and immunoglobulin-binding protein–antibodies–labeled antigen (Scheme C). The lowest detection limit was observed for Scheme C, and was equal to 10 ng/mL of specific humanized monoclonal antibodies. When working with pooled positive sera, Scheme C had a detection limit 15 times lower than Scheme B and 255 times lower than Scheme A. Due to the high sensitivity of Scheme C, its application for the panel of human sera (n = 22) demonstrated 100% diagnostic specificity and sensitivity. These consistent results be useful for designing the format of LFIA serodiagnosis for other diseases.
Effectiveness of an Intervention to Enhance First Aid Knowledge among Early Childhood Education Students: A Pilot Study
Authors: León-Guereño Patxi , Cid-Aldama Laura , Galindo-Domínguez Héctor , Amezua-Urrutia Alaitz
Published: July 2023

Empowering early childhood education students from the beginning with the necessary knowledge and skills to act swiftly in emergency situations could be crucial in saving lives in certain cases. In order to improve the first aid knowledge and skills of early childhood education students, a pre/post study was conducted with a two-week intermediate intervention involving 13 early childhood education students. Their knowledge and skills in first aid were assessed using an ad-hoc instrument before and after the intervention. The results demonstrate a statistically significant improvement in all items related to first aid general knowledge, first aid kits, and CPR maneuvers, as well as in the overall scale. These findings provide evidence that early childhood education students can be equipped through low-cost interventions to acquire and apply certain essential first aid skills, such as dialing emergency services, understanding the purpose of first aid kit items, and recognizing vital signs in individuals, that may be crucial in saving lives in the future.
Evaluation of Palatal Bone Thickness at the Implantation Areas of Two Popular Bone-Anchored Distalizers—A Cone Beam Computed Tomography Retrospective Study
Authors: Gibas-Stanek Marta , Żabicki Szczepan , Urzędowski Michał , Pihut Małgorzata
Published: July 2023

Since class II malocclusion and lack of space within the dental arch due to early loss of deciduous molars is a common orthodontic problem in the Polish population, bone-anchored distalizers are becoming more and more popular. The aim of the present study was to evaluate palatal soft and hard tissue thickness using cone beam computed tomography (CBCT) at the area of micro-implant placement of two appliances for maxillary first molar distalization: Beneslider and TopJet distalizer. The study data were 100 consecutively selected CBCT images (53 of men and 47 of women). Measurements of bone and mucosa thickness were performed at six locations in the palate and tested according to their correlation with sex and age. The biggest bone and mucosa thickness were recorded in the insertion site of the TopJet miniscrew. Bone thickness in all points of paramedian insertion was significantly greater in males and the mean difference was approximately 1–1.8 mm. Age correlates significantly (p < 0.05) and positively (r > 0) with the thickness of the mucosa at all points: the older the patient, the thicker the mucosa at each measurement point. Anatomical diversity of the hard palate in the population involves the need to perform bone and mucosa thickness measurements before palatal micro-implant placement.
Three-Dimensional Spinal Evaluation Using Rasterstereography in Patients with Adolescent Idiopathic Scoliosis: Is It Closer to Three-Dimensional or Two-Dimensional Radiography?
Authors: Tabard-Fougère Anne , de Bodman Charlotte , Dhouib Amira , Bonnefoy-Mazure Alice , Armand Stéphane , Dayer Romain
Published: July 2023

(1) Background: Adolescent Idiopathic Scoliosis (AIS) is a three-dimensional (3D) spine deformity. The Cobb angle, evaluated with 2D radiography, is the gold standard to determine curve severity. The primary aim of this study was to evaluate the 3D spinal evaluation with rasterstereography in patients with AIS. The hypothesis was that rasterstereography reached higher accuracy than the gold standard 2D radiography. The second aim was to compare rasterstereography with 3D radiography. The hypothesis was that the rasterstereographic evaluation of patients with severe major scoliosis curves is closer to 3D radiography compared to the gold standard (2D radiography). (2) Methods: This is a prospective comparative study of a consecutive series of 53 patients, with the scoliosis curve evaluated with two 3D methods and the gold standard (2D radiography). (3) Results: The hypothesis that rasterstereography reached higher accuracy than the gold standard 2D radiography was validated for all curves. Even if all curves were highly correlated, both rasterstereography and 2D radiography scoliosis evaluation were underestimated for moderate/severe curves compared to 3D radiography. (4) Conclusions: The rasterstereographic evaluation of major curve scoliosis is not accurate enough to replace 2D radiography for moderate/severe curves. A longitudinal follow-up should be assessed in future studies to define the sensitivity of the detection of a significant change in the scoliotic mild and moderate curve (<40°).
The TEK Design Principles: Integrating Neuroscience and Learning Environment Research
Authors: Tan Aik Lim , Gillies Robyn , Jamaludin Azilawati
Published: July 2023

People engage in learning in various settings and environments. This involves learning in a formal learning environment like a classroom and less formal environments such as museums and online. Interest is one of the key motivational theories and plays an important role in learning, as it can not only initiate and propel individuals to pursue an area of knowledge but also motivate them to maintain this pursuit in the long term. There has been a lack of research in the field of the design of learning spaces to support student-initiated knowledge generation. Coupled with the emerging area of neuroscience and education, this paper aims to synthesise neuroscience research with aspects of learning design to facilitate learning and interest development within a high school learning context. The Task, Environment and Knowledge Creation (TEK) design principles are proposed. Implications of the design principles are discussed.
Explainable Ensemble Learning Based Detection of Evasive Malicious PDF Documents
Authors: Yerima Suleiman Y. , Bashar Abul
Published: July 2023

PDF has become a major attack vector for delivering malware and compromising systems and networks, due to its popularity and widespread usage across platforms. PDF provides a flexible file structure that facilitates the embedding of different types of content such as JavaScript, encoded streams, images, executable files, etc. This enables attackers to embed malicious code as well as to hide their functionalities within seemingly benign non-executable documents. As a result, a large proportion of current automated detection systems are unable to effectively detect PDF files with concealed malicious content. To mitigate this problem, a novel approach is proposed in this paper based on ensemble learning with enhanced static features, which is used to build an explainable and robust malicious PDF document detection system. The proposed system is resilient against reverse mimicry injection attacks compared to the existing state-of-the-art learning-based malicious PDF detection systems. The recently released EvasivePDFMal2022 dataset was used to investigate the efficacy of the proposed system. Based on this dataset, an overall classification accuracy greater than 98% was observed with five ensemble learning classifiers. Furthermore, the proposed system, which employs new anomaly-based features, was evaluated on a reverse mimicry attack dataset containing three different types of content injection attacks, i.e., embedded JavaScript, embedded malicious PDF, and embedded malicious EXE. The experiments conducted on the reverse mimicry dataset showed that the Random Committee ensemble learning model achieved 100% detection rates for embedded EXE and embedded JavaScript, and 98% detection rate for embedded PDF, based on our enhanced feature set.
A Fixed-Point Position Observation Algorithm and System-on-Chip Design Suitable for Sensorless Control of High-Speed Permanent Magnet Synchronous Motor
Authors: He Changjun , Xu Shiyun , Yan Bosong , Wang Zhenhua , Wang Mingjiang
Published: July 2023

In the sensorless control algorithm of permanent magnet synchronous motors (PMSMs), there is a significant research focus on developing a high-performance position observer and a cost-effective, high-performance processor specifically designed for PMSMs. In this paper, we first reviewed the current mainstream position observation algorithms. Subsequently, we proposed a position observation algorithm based on a fixed-point nonlinear flux linkage model that introduces a variable gain model and an observer compensation model. Furthermore, we implemented the improved position observation algorithm in the circuit and designed a System-on-Chip (SOC) suitable for the sensorless control of high-speed permanent magnet synchronous motors using the ARM Cortex-M0 core. The functionality of the proposed SOC was verified on the Field Programmable Gate Array (FPGA) platform. The results demonstrate that the improved algorithm offers an excellent angle observation accuracy and fast convergence speed. The error between the observed angle and the actual angle is less than 0.2 rad. Additionally, the designed SOC balances the high efficiency of hardware implementation with the flexibility of software implementation. When running a sensorless control algorithm for PMSM, it achieves a 30.3% increase in execution efficiency, enabling support for higher frequency output PWM switching and making it suitable for the high-precision control of high-speed PMSM.
Wind Farms and Flexible Loads Contribution in Automatic Generation Control: An Extensive Review and Simulation
Authors: Ullah Kaleem , Ullah Zahid , Aslam Sheraz , Salam Muhammad Salik , Salahuddin Muhammad Asjad , Umer Muhammad Farooq , Humayon Mujtaba , Shaheer Haris
Published: July 2023

With the increasing integration of wind energy sources into conventional power systems, the demand for reserve power has risen due to associated forecasting errors. Consequently, developing innovative operating strategies for automatic generation control (AGC) has become crucial. These strategies ensure a real-time balance between load and generation while minimizing the reliance on operating reserves from conventional power plant units. Wind farms exhibit a strong interest in participating in AGC operations, especially when AGC is organized into different regulation areas encompassing various generation units. Further, the integration of flexible loads, such as electric vehicles and thermostatically controlled loads, is considered indispensable in modern power systems, which can have the capability to offer ancillary services to the grid through the AGC systems. This study initially presents the fundamental concepts of wind power plants and flexible load units, highlighting their significant contribution to load frequency control (LFC) as an important aspect of AGC. Subsequently, a real-time dynamic dispatch strategy for the AGC model is proposed, integrating reserve power from wind farms and flexible load units. For simulations, a future Pakistan power system model is developed using Dig SILENT Power Factory software (2020 SP3), and the obtained results are presented. The results demonstrate that wind farms and flexible loads can effectively contribute to power-balancing operations. However, given its cost-effectiveness, wind power should be operated at maximum capacity and only be utilized when there is a need to reduce power generation. Additionally, integrating reserves from these sources ensures power system security, reduces dependence on conventional sources, and enhances economic efficiency.
Controlling the Reactive Power Demand of a Distribution Grid by Coordinated Action of Electric Vehicle Chargers
Authors: Schilcher Matthias , Neff Sebastian , Muenderlein Jeanette
Published: July 2023

Various factors, particularly an increasing share in underground cable lines, can lead to excessive reactive power demand of distribution grids to their respective transport grids and, thus, to the need of costly compensation measures. At the same time, the occurrence of battery-electric cars is considerably increasing. It is, therefore, conceivable to provide reactive power as an inexpensive “waste product” by the charging converters of the cars as a solution to the aforementioned problem. In this research work, the idea is examined for its feasibility by means of extensive time series simulation using the example of a real existing distribution grid. It was found that, with a realistic number of charging points—especially public ones with integrated converter electronics—not only can the reactive power demand be reduced to an acceptable level but also additional reactive power can be provided as a service to the transport grid.
A Review on the Fault and Defect Diagnosis of Lithium-Ion Battery for Electric Vehicles
Authors: Zou Bosong , Zhang Lisheng , Xue Xiaoqing , Tan Rui , Jiang Pengchang , Ma Bin , Song Zehua , Hua Wei
Published: July 2023

The battery system, as the core energy storage device of new energy vehicles, faces increasing safety issues and threats. An accurate and robust fault diagnosis technique is crucial to guarantee the safe, reliable, and robust operation of lithium-ion batteries. However, in battery systems, various faults are difficult to diagnose and isolate due to their similar features and internal coupling relationships. In this paper, the current research of advanced battery system fault diagnosis technology is reviewed. Firstly, the existing types of battery faults are introduced in detail, where cell faults include progressive and sudden faults, and system faults include a sensor, management system, and connection component faults. Then, the fault mechanisms are described, including overcharge, overdischarge, overheat, overcool, large rate charge and discharge, and inconsistency. The existing fault diagnosis methods are divided into four main types. The current research and development of model-based, data-driven, knowledge-based, and statistical analysis-based methods for fault diagnosis are summarized. Finally, the future development trend of battery fault diagnosis technology is prospected. This paper provides a comprehensive insight into the fault and defect diagnosis of lithium-ion batteries for electric vehicles, aiming to promote the further development of new energy vehicles.
Energy Storage and Release of Class I and Class II Rocks
Authors: Xing Yan , Gao Feng , Zhang Zhizhen , Zheng Wenqi
Published: July 2023

As underground excavations become deeper, violent rock failures associated with the sudden release of elastic energy become more prevalent, threatening the safety of workers and construction equipment. It is important to figure out the energy-related failure mechanisms of rocks. However, the energy evolution across the complete deformation of different types of rocks and the effect of high confinement on energy storage and release are not well understood in the literature. In this study, a series of cyclic triaxial compression tests were conducted for Class I and Class II rocks to investigate the confinement-dependent characteristics of energy evolution. The results showed that three types of energy evolution were identified as the rock behavior changed from brittle to ductile. The energy storage limit was linearly enhanced by confinement. The nonlinear increase in dissipated energy at peak stress with increasing confinement was suggested to indicate the start of the brittle–ductile transition. The post-peak fracturing process was characterized using the ratio of the local withdrawn elastic energy and fracture energy, and a novel energy-based index was proposed to quantify the failure intensity of the rock. This paper presents a complete investigation of the energy conversion characteristics of the rock, which may shed light on the failure mechanisms of violent rock failures in underground projects.
Investigating the Anaerobic Digestion of Water Hyacinth (Eichhornia crassipes) Sourced from Hartbeespoort Dam in South Africa
Authors: Simbayi Trevor M. , Rashama Charles , Awosusi Ayo A. , Nkuna Rosina , Christian Riann , Matambo Tonderayi S.
Published: July 2023

The biodegradability of water hyacinth for biogas and biofertilizer production was studied under mesophilic conditions. The effects of water hyacinth pretreatments were also included in this investigation. It was found that water hyacinth has a low biodegradability of 27% when monodigested, while in a 3:1 ratio with cow manure, the biodegradability increases to 46%. At this elevated biodegradability, the water hyacinth biomethane potential was 185 LCH4/kgVS, while that of cow manure was 216 LCH4/kgVS. The Gompertz kinetic model had superior parameters than the logistic model for most of the water hyacinth–cow manure combined substrate digestion. Based on the Gompertz model, the lag phase and daily maximum methane production rate were 5.5 days and 22.9 mL/day, respectively, for the 3:1 codigestion (R2 of 0.99). These values were 6.7 days and 15.2 mL/day, respectively, in the case of water hyacinth monodigestion (R2 = 0.996). The dominant microbial species detected in the digestates were Bacteroidetes and Proteobacteria. A few microbial species were indigenous to water hyacinth, but more diverse consortia, which are key to efficient substrate biodegradation, came from cow manure. The digestate contained ammonium nitrogen at 68 mg/kg with phosphorous and potassium at 73 and 424 mg/kg, respectively. Nitrogen was lower but phosphorous and potassium were comparable to previously studied digestates of other substrates. Only water hyacinth pretreated by aerobic composting was proven to unlock a higher methane yield that matched the 3:1 codigestion with cow manure. Other pretreatments induced better biodegradation performance than that observed in untreated water hyacinth but these improvements were not as good as that of the 3:1 codigestion scheme. It was concluded that water hyacinth sourced from the Hartbeespoort Dam could be treated by anaerobic digestion to recover biogas and biofertilizer. However, more experiments are required to fully understand and harness the optimisation opportunities available in applying this technology to manage water hyacinths.
Combined Treatment of Acidic Electrolyzed Water and High-Voltage Electrostatic Field Improves the Storage Quality of Huping Jujube (Ziziphus jujuba Mill. cv. Huping)
Authors: Chang Xiaojie , Liang Yueguang , Guo Tianjing , Wang Yu , Yang Jiali
Published: July 2023

Fresh jujube is prone to rapid deterioration after harvest due to its active metabolism and rich nutrients. This study aimed to investigate the effects of acidic electrolyzed water (AEW), a high-voltage electrostatic field (HVEF) and a combination of AEW and HVEF (AEW + HVEF) treatments on the storage quality of Huping jujube (Ziziphus jujuba Mill. cv. Huping) stored at 0 ± 1 °C for 90 days. The results showed that the fruits treated with AEW + HVEF exhibited better storage quality than those treated with either AEW or HVEF alone. Specifically, the fruits treated with AEW + HVEF maintained higher levels of nutrients and taste compounds, including total soluble solid (TSS), total soluble sugar, reducing sugar and titratable acidity (TA), as well as lower respiration rate, weight loss, decay index and TSS/TA ratio. Additionally, the AEW + HVEF treatment could delay the increase in reddening index, a* and color change (ΔE) values, and the decrease in L* and b* values, by retarding the degradation of chlorophyll and accumulation of carotenoids and flavonoids, thereby preserving the more desirable appearance color. Furthermore, the combined treatment could enhance the glutathione reductase (GR) activity and 2,2′-azino-bis-(3-ethylbenzothizoline)−6-sulfonic acid (ABTS) +-scavenging ability. Thus, the AEW + HVEF treatment is a potential method for Huping jujube preservation.
Development of Magnetic Lateral Flow and Direct Competitive Immunoassays for Sensitive and Specific Detection of Halosulfuron-Methyl Using a Novel Hapten and Monoclonal Antibody
Authors: Ying Ying , Cui Xueyan , Li Hui , Pan Lingyi , Luo Ting , Cao Zhen , Wang Jing
Published: July 2023

Halosulfuron-methyl (HM) is widely used for the removal of noxious weeds in corn, sugarcane, wheat, rice, and tomato fields. Despite its high efficiency and low toxicity, drift to nontarget crops and leaching of its metabolites to groundwater pose potential risks. Considering the instability of HM, the pyrazole sulfonamide of HM was used to generate a hapten and antigen to raise a high-quality monoclonal antibody (Mab, designated 1A91H11) against HM. A direct competitive immunoassay (dcELISA) using Mab 1A91H11 achieved a half-maximal inhibitory concentration (IC50) of 1.5 × 10−3 mg/kg and a linear range of 0.7 × 10−3 mg/kg–10.7 × 10−3 mg/kg, which was 10 times more sensitive than a comparable indirect competitive ELISA (icELISA) and more simple to operate. A spiking recovery experiment performed in tomato and maize matrices with 0.01, 0.05, and 0.1 mg/kg HM had average recoveries within 78.9–87.9% and 103.0–107.4% and coefficients of variation from 1.1–6.8% and 2.7–6.4% in tomato and maize, respectively. In addition, a magnetic lateral flow immunoassay (MLFIA) was developed for quantitative detection of low concentrations of HM in paddy water. Compared with dcELISA, the MLFIA exhibited 3.3- to 50-fold higher sensitivity (IC50 0.21 × 10−3 mg/kg). The average recovery and RSD of the developed MLFIA ranged from 81.5 to 92.5% and 5.4 to 9.7%. The results of this study demonstrated that the developed dcELISA and MLFIA are suitable for rapid detection of HM residues in tomato and maize matrices and paddy water, respectively, with acceptable accuracy and precision.
Whey Protein–Tannic Acid Conjugate Stabilized Emulsion-Type Pork Sausages: A Focus on Lipid Oxidation and Physicochemical Features
Authors: Aewsiri Tanong , Ganesan Palanivel , Thongzai Hataikan
Published: July 2023

The purpose of this study was to investigate the oxidative stability and physicochemical properties of pork emulsion sausages with whey protein–tannic acid conjugate and native whey protein. Over the course of 21 days, the thiobarbituric acid reactive substances (TBARS) of sausages containing a whey protein–tannic acid conjugate were lower than those of sausages with regular whey protein (p < 0.05). Kinetically, sausage containing the whey protein–tannic acid conjugate (k = 0.0242 day−1) appeared to last longer than sausage containing regular whey protein (k = 0.0667 day−1). The addition of the whey protein–tannic acid conjugate had no effect on product texture because there was no difference in hardness, springiness, cohesiveness, or water-holding capacity between the control and treated samples at Day 0 (p > 0.05). Scanning electron microscopy revealed that, at Day 21, the control sausage exhibited emulsion coalescence, as evidenced by an increase in the number of oil droplets and large voids, but not the whey protein–tannic acid conjugate-added sausage. There was no variation in the L*, a*, and b* values of the sausages when the whey protein–tannic acid conjugate was added (p > 0.05). However, there was a little increase in ΔE value in the treated sample. Thus, the whey-protein–tannic acid conjugate appeared to stabilize the lipid and physicochemical properties of the sausages by lowering the rate of TBARS production, retaining texture, water-holding capacity, and color, as well as by minimizing lipid coalescence during refrigerated storage.
A Novel Foodborne Illness Detection and Web Application Tool Based on Social Media
Authors: Tao Dandan , Hu Ruofan , Zhang Dongyu , Laber Jasmine , Lapsley Anne , Kwan Timothy , Rathke Liam , Rundensteiner Elke , Feng Hao
Published: July 2023

Foodborne diseases and outbreaks are significant threats to public health, resulting in millions of illnesses and deaths worldwide each year. Traditional foodborne disease surveillance systems rely on data from healthcare facilities, laboratories, and government agencies to monitor and control outbreaks. Recently, there is a growing recognition of the potential value of incorporating social media data into surveillance systems. This paper explores the use of social media data as an alternative surveillance tool for foodborne diseases by collecting large-scale Twitter data, building food safety data storage models, and developing a novel frontend foodborne illness surveillance system. Descriptive and predictive analyses of the collected data were conducted in comparison with ground truth data reported by the U.S. Centers for Disease Control and Prevention (CDC). The results indicate that the most implicated food categories and the distributions from both Twitter and the CDC were similar. The system developed with Twitter data could complement traditional foodborne disease surveillance systems by providing near-real-time information on foodborne illnesses, implicated foods, symptoms, locations, and other information critical for detecting a potential foodborne outbreak.
Laccase-Induced Gelation of Sugar Beet Pectin–Curcumin Nanocomplexes Enhanced by Genipin Crosslinking
Authors: Lin Jia-Wei , Jiang Gui-Li , Liang Cui-Xin , Li Ye-Meng , Chen Xing-Yi , Zhang Xiao-Tong , Tang Zhong-Sheng
Published: July 2023

Research on the use of polysaccharides as hydrophobic bioactive carriers instead of proteins is still scarce. Sugar beet pectin (SBP) contains a small amount of protein and is a potential carrier for loading curcumin. In this work, SBP encapsulation, genipin crosslinking, and laccase-induced gelation were used to develop novel jelly food and improve the stability of curcumin without the incorporation of oil. By mixing the SBP solution (40 mg/mL) with curcumin powder (25 mg/mL SBP solution), an SBP–curcumin complex (SBP–Cur) was fabricated with a loading amount of 32 mg/g SBP, and the solubility of curcumin improved 116,000-fold. Fluorescence spectroscopy revealed that hydrophobic interactions drove the complexation of curcumin and SBP. Crosslinked by genipin (10 mM), SBP–Cur showed a dark blue color, and the gel strength of laccase-catalyzed gels was enhanced. Heating and UV radiation tests suggested that the genipin crosslinking and gelation strategies substantially improved the stability of curcumin. Because of the unique UV-blocking capacity of blue pigment, crosslinked samples retained 20% more curcumin than control samples. With the enhanced stability of curcumin, the crosslinked SBP–curcumin complexes could be a functional food ingredient used in functional drinks, baked food, and jelly food.
Evaluating Classification Systems of Diabetic Foot Ulcer Severity: A 12-Year Retrospective Study on Factors Impacting Survival
Authors: Niță Otilia , Arhire Lidia Iuliana , Mihalache Laura , Popa Alina Delia , Niță George , Gherasim Andreea , Graur Mariana
Published: July 2023

(1) Background: This study examines the survival of patients after their first presentation with diabetic foot ulcers (DFUs) to the regional Diabetes, Nutrition, and Metabolic Diseases Clinic within the Emergency Clinical Hospital “Sf. Spiridon”, Iaşi, and analyzes the factors associated with this outcome. (2) Methods: In this retrospective study, patients with DFUs consecutively referred between 1 January 2007 and 31 December 2017 were followed up until 31 December 2020 (for 13 years). The study group included 659 subjects. (3) Results: During the study period, there were 278 deaths (42.2%) and the average survival time was 9 years. The length of hospitalization, diabetic nephropathy, chronic kidney disease, glomerular filtration rate, cardiovascular disease, hypertension, anemia, and DFU severity were the most significant contributors to the increase in mortality. Patients with severe ulcers, meaning DFUs involving the tendon, joint, or bone, had a higher mortality risk than those with superficial or pre-ulcerative lesions on initial presentation (Texas classification HR = 1.963, 95% CI: 1.063–3.617; Wagner–Meggitt classification HR = 1.889, 95% CI: 1.024–3.417, SINBAD Classification System and Score HR = 2.333, 95% CI: 1.258–4.326) after adjusting for confounding factors. (4) Conclusions: The findings of this study suggested that patients presenting with severe ulcers involving the tendon, joint, or bone exhibited a significantly higher risk of mortality, even when potential confounders were taken into consideration.
Estimation of a Structural Equation Modeling of Quality of Life Mediated by Difficulty in Daily Life in Survivors of Breast Cancer
Authors: Watanabe Aki , Kawaguchi Takayuki , Nobematsu Ayumi , Sasada Satoshi , Kanari Nozomi , Maru Tatsuya , Kobayashi Takeshi
Published: July 2023

Background: The purpose of this study was to clarify the structural relationship of quality of life (QOL) in survivors of breast cancer, including difficulty in daily life and negative experiences in daily activities, as health-related indicators. Methods: Participants were survivors of breast cancer for more than 2 years after primary breast cancer surgery and belonged to self-help groups. The assessment used FACT-B (QOL), HADS (anxiety and depression), SOC (sense of coherence), WHODAS 2.0 (difficulties in daily life), and CAOD (negative experiences in daily activities). Bayesian structural equation modeling (BSEM) was performed to analyze the hypothesized model. If the causal model was significant, multiplication of the path coefficient from emotional distress (anxiety and depression) to QOL, and from SOC to emotional distress, was considered a direct effect on QOL, and from SOC to difficulty in daily life, from difficulty in daily life to negative experiences in daily activities, and from negative experiences in daily activities to anxiety and depression were considered indirect effects on QOL. Results: The participants comprised 73 survivors of breast cancer. The goodness of fit of the model in the BSEM was satisfactory. The direct effect was 0.274, and the indirect effect was 0.164. Conclusions: An additional finding of this study is that coping with difficulty in daily life and negative experiences in daily activities related to QOL may improve QOL.
Metabolic, Apoptotic and Fibro-Inflammatory Profiles of the Heart Exposed to Environmental Electromagnetic Fields
Authors: Savchenko Lesia , Martinelli Ilenia , Marsal Dimitri , Batkivska Oksana , Zhdan Vyacheslav , Kaidashev Igor , Pizzinat Nathalie , Boal Frederic , Tronchere Helene , Tao Junwu , Kunduzova Oksana
Published: July 2023

Environmental stress can disturb the integrative functioning of the cardiovascular system and trigger a number of adaptive and/or maladaptive cell responses. Concomitant with the expanding use of mobile communication systems, public exposure to electromagnetic fields (EMFs) raises the question of the impact of 900 MHz EMFs on cardiovascular health. Therefore, in this study, we experimentally investigated whether 915 MHz EMF exposure influenced cardiac metabolic, antioxidant, apoptotic, and fibro-inflammatory profiles in a mouse model. Healthy mice were sham-exposed or exposed to EMF for 14 days. Western blot analysis using whole cardiac tissue lysates demonstrated that there was no significant change in the expression of oxidative phosphorylation (OXPHOS) complexes between the control and EMF-exposed mice. In addition, the myocardial expression of fibro-inflammatory cytokines, antioxidant enzymes, and apoptosis-related markers remained unchanged in the EMF-challenged hearts. Finally, the structural integrity of the cardiac tissues was preserved among the groups. These findings suggest that the apoptotic, antioxidant, metabolic, and fibro-inflammatory profiles of the heart remained stable under conditions of EMF exposure in the analyzed mice.
Mesenchymal Stem Cell Therapy in Traumatic Spinal Cord Injury: A Systematic Review
Authors: Montoto-Meijide Rodrigo , Meijide-Faílde Rosa , Díaz-Prado Silvia María , Montoto-Marqués Antonio
Published: July 2023

Recovery from a traumatic spinal cord injury (TSCI) is challenging due to the limited regenerative capacity of the central nervous system to restore cells, myelin, and neural connections. Cell therapy, particularly with mesenchymal stem cells (MSCs), holds significant promise for TSCI treatment. This systematic review aims to analyze the efficacy, safety, and therapeutic potential of MSC-based cell therapies in TSCI. A comprehensive search of PUBMED and COCHRANE databases until February 2023 was conducted, combining terms such as “spinal cord injury,” “stem cells,” “stem cell therapy,” “mesenchymal stem cells,” and “traumatic spinal cord injury”. Among the 53 studies initially identified, 22 (21 clinical trials and 1 case series) were included. Findings from these studies consistently demonstrate improvements in AIS (ASIA Impairment Scale) grades, sensory scores, and, to a lesser extent, motor scores. Meta-analyses further support these positive outcomes. MSC-based therapies have shown short- and medium-term safety, as indicated by the absence of significant adverse events within the studied timeframe. However, caution is required when drawing generalized recommendations due to the limited scientific evidence available. Further research is needed to elucidate the long-term safety and clinical implications of these advancements. Although significant progress has been made, particularly with MSC-based therapies, additional studies exploring other potential future therapies such as gene therapies, neurostimulation techniques, and tissue engineering approaches are essential for a comprehensive understanding of the evolving TSCI treatment landscape.
Natural Syntax, Artificial Intelligence and Language Acquisition
Authors: O’Grady William , Lee Miseon
Published: July 2023

In recent work, various scholars have suggested that large language models can be construed as input-driven theories of language acquisition. In this paper, we propose a way to test this idea. As we will document, there is good reason to think that processing pressures override input at an early point in linguistic development, creating a temporary but sophisticated system of negation with no counterpart in caregiver speech. We go on to outline a (for now) thought experiment involving this phenomenon that could contribute to a deeper understanding both of human language and of the language models that seek to simulate it.
Conventional vs. Sutureless Aortic Valve Bioprosthesis: Is Faster Better?
Authors: Aljalloud Ali , Moza Ajay , Arias Jessica Paola , Menne Matthias , Becker Michael , Spetsotaki Konstantina
Published: July 2023

Purpose: The benefits of sutureless compared to conventional aortic valve prosthesis replacement remain controversial. Supposed advantages of sutureless aortic valve replacement include shortened cross-clamp and implantation time, as well as improved overall safety and good post-operative performance. We aimed to compare the early outcomes and performance of sutureless aortic valve replacement (su-AVR) with the sutureless Perceval (Corcym, Milan, Italy) vs. the conventional AVR with a conventional counterpart, in this case, the Labcor Dokimos Plus (LDP) aortic bioprosthesis. Methods: We compared two types of aortic valve prostheses, the sutureless (Corcym, Milan, Italy) and the conventional valve Labcor Dokimos Plus (LDP), implanted between August 2014 and May 2019 in our Department of Cardiac Surgery at RWTH Aachen University Hospital. Data were collected from 141 patients who received the Perceval (Corcym, Milan, Italy) and 138 who received the Labcor Dokimos Plus (LDP) aortic bioprosthesis. After matching the two groups considering STS mortality risk and pre-operative LDH levels, 201 patients were included in our final study cohort. Seventy-one patients (17 from the Perceval group and 54 from the Dokimos group) were excluded due to the lack of complete data, particularly standardized echocardiographic data (n = 71). Primary endpoints were 30-day mortality, length of hospital stay, and pacemaker implantation. Secondary endpoints were echocardiographic parameters, major adverse cardiovascular events, and prosthesis failure (grade II aortic regurgitation, paravalvular leak with reintervention). Results: Bypass and cross-clamp time proved to be shorter in the Perceval group, while hospital stays were longer. The faster implantation had no effect on the 30-day mortality primary endpoint. Transvalvular gradients were significantly higher in the Perceval group, in addition to a smaller effective orifice area. The LDH values were remarkably higher post-operatively in the Perceval group. Conclusions: Regarding the clinical outcomes, Perceval was equivalent and not superior to the Dokimus bioprosthesis. The suitability of a Perceval prosthesis implantation must be determined on a case-by-case basis and reserved for elderly patients with increased comorbidity.
Perioperative Application of Levosimendan Optimizes Postoperative Renal Function and Organ Perfusion in Patients with Severe Heart Failure
Authors: Leivaditis Vasileios , Dahm Manfred , Papaporfyriou Anastasia , Galanis Michail , Koletsis Efstratios , Charokopos Nikolaos , Ehle Benjamin , Papatriantafyllou Athanasios , Haussmann Erich , Kaplunov Vladislav , Grapatsas Konstantinos
Published: July 2023

Background: Renal dysfunction and impaired organ perfusion are common concerns following cardiac surgery. Levosimendan, a calcium sensitizer inotropic drug, is investigated in this study for its potential to improve postoperative renal function and organ perfusion in patients with low preoperative ejection fraction and severe myocardial dysfunction after cardiac surgery. Methods: A retrospective analysis was conducted on 314 patients with preoperative heart failure who underwent cardiac surgery. Among them, 184 patients received perioperative adjunctive therapy with levosimendan, while 130 patients with similar characteristics received conventional treatment. Results: The perioperative administration of levosimendan resulted in a significantly lower need for renal replacement therapy (p < 0.001) and improvements in the serum creatinine levels, glomerular filtration rate, and creatinine clearance. Similarly, the C-reactive protein levels, blood pH, and lactic acid levels showed comparable improvements. Conclusions: The use of levosimendan was associated with a significant enhancement in postoperative renal function and a reduction in the need for renal replacement therapy. Furthermore, it resulted in a decrease in the extent of organ malperfusion. Postoperative inflammatory reactions and metabolic balance also exhibited improvements.
Risk Factors for the In-Hospital Mortality in Pyogenic Vertebral Osteomyelitis: A Cross-Sectional Study on 9753 Patients
Authors: Ziarko Tomasz Piotr , Walter Nike , Schindler Melanie , Alt Volker , Rupp Markus , Lang Siegmund
Published: July 2023

Background: Pyogenic vertebral osteomyelitis represents a clinical challenge associated with significant morbidity and mortality. The aim of this study was to analyze potential risk factors for the in-hospital mortality of vertebral osteomyelitis (VO) patients. Methods: Based on the International Classification of Diseases, 10th Revision (ICD-10) codes for VO (“M46.2-”, “M46.3-”, and “M46.4-”) data for total case numbers, secondary diagnoses, and numbers of in-hospital deaths were extracted from the Institute for the Hospital Remuneration System (InEK GmbH). Odds ratios (OR) for death were calculated for several secondary diseases and factors of interest. Results: Despite age, certain comorbidities were found to be strongly associated with increased mortality risk: Heart failure (OR = 2.80; 95% CI 2.45 to 3.20; p < 0.01), chronic kidney disease (OR = 1.83; 95% CI 1.57 to 2.13; p < 0.01), and diabetes with complications (OR = 1.86; 95% CI 1.46 to 2.38; p < 0.01). Among the complications, acute liver failure showed the highest risk for in-hospital mortality (OR = 42.41; 95% CI 23.47 to 76.62; p < 0.01). Additionally, stage III kidney failure (OR = 9.81; 95% CI 7.96 to 12.08; p < 0.01), sepsis (OR = 5.94; 95% CI 5.02 to 7.03; p < 0.01), acute respiratory failure (OR = 5.31; 95% CI 4.61 to 6.12; p < 0.01), and systemic inflammatory response syndrome (SIRS) (OR = 5.19; 95% CI 3.69 to 5.19; p < 0.01) were associated with in-hospital mortality. When analyzing the influence of pathogens, documented infection with Pseudomonas aeruginosa had the highest risk for mortality (OR = 2.74; 95% CI 2.07 to 3.63; p < 0.01), followed by Streptococci, Escherichia coli, and Staphylococcus aureus infections. Conclusions: An early assessment of individual patient risk factors may be beneficial in the care and treatment of VO to help reduce the risks of mortality. These findings emphasize the importance of closely monitoring VO patients with chronic organ diseases, early detection and treatment of sepsis, and tailored empirical antibiotic therapy. The identification of specific pathogens and antibiotic susceptibility testing should be prioritized to improve patient outcomes in this high-risk population.
Coupled Analysis of Hydrodynamic Responses of a Small Semi-Submersible Platform and a Large Floating Body
Authors: Yang Jianye , Yan Jun , Zhao Yan , Chen Jinlong , Jin Heng
Published: July 2023

This paper focuses on the hydrodynamic interaction between the large floating body and a small transfer platform in a jettyless floating transfer system. A high-order boundary-element method combined with a direct time-domain-solution method to calculate and analyze the hydrodynamic response of the small platform while berthed with the fixed large floating body and freely floating large body under wave action was applied and compared with the hydrodynamic response of a single small transfer platform. It was found that when the large floating body and the small transfer platform were both located on the leeward side, they had little mutual influence, and the hydrodynamic response agreed well with that of the single small transfer platform and the single large floating body. While the small platform was located on the leeward side of the large floating body, it significantly affected the hydrodynamic response of the platform, resulting in a significant reduction in horizontal motion and pitch motion, meaning that the shielding effect was significant. Within a certain range of wave periods, the heave-motion amplitudes can be significantly reduced, but beyond that range, they increase. Therefore, it is important to carefully consider the relative motions of LNG transport ships and small platforms when connecting them via aerial jumper pipes in jettyless floating transfer systems.
Candida palmioleophila: A New Emerging Threat in Brazil?
Authors: Costa Gisela Lara da , Negri Melyssa , Miranda Rodrigo Prado Rodrigues de , Corrêa-Moreira Danielly , Pinto Tatiana Castro Abreu , Ramos Livia de Souza , Ferreira Deisiany Gomes , Salomão Bruna , Fumian Tulio Machado , Mannarino Camille Ferreira , Prado Tatiana , Miagostovich Marise Pereira , Santos André Luis Souza dos , Oliveira Manoel Marques Evangelista
Published: July 2023

Human activity directly or indirectly causes climate change, promoting changes in the composition of the atmosphere. This change is beyond the variation of the natural climate. In this manner, climate change could create an environmental pressure which is enough to trigger new fungal diseases. In addition to climate alterations, the onset of the COVID-19 pandemic has also been associated with the emergence of fungal pathogens. Fungi showed that an inability to grow at high temperatures limits the capacity of fungi to infect mammals. However, fungi can develop thermotolerance, gradually adapting to rising temperatures due to climate change, and generating a greater number of disease-causing organisms. In the present study, we reported the detection and identification of Candida palmioleophila isolates recovered from raw sewage samples in Niteroi city, Rio de Janeiro State, Brazil, during a monitoring program for measuring SARS-CoV-2 presence and concentration. Using polyphasic taxonomy to identify the species and evaluating some virulence aspects of this species, such as biofilm formation and extracellular enzyme production, our data highlight this species as a possible emerging pathogen in Brazil, especially in the pandemic context.
The First Exploratory Personalized Medicine Approach to Improve Bariatric Surgery Outcomes Utilizing Psychosocial and Genetic Risk Assessments: Encouraging Clinical Research
Authors: Thanos Panayotis K. , Hanna Colin , Mihalkovic Abrianna , Hoffman Aaron B. , Posner Alan R. , Busch John , Smith Caroline , Badgaiyan Rajendra D. , Blum Kenneth , Baron David , Mastrandrea Lucy D. , Quattrin Teresa
Published: July 2023

It is predicted that by 2030, globally, an estimated 2.16 billion adults will be overweight, and 1.12 billion will be obese. This study examined genetic data regarding Reward Deficiency Syndrome (RDS) to evaluate their usefulness in counselling patients undergoing bariatric surgery and gathered preliminary data on the potential use in predicting short term (6-month) weight loss outcomes. Methods: Patients undergoing bariatric surgery (n = 34) were examined for Genetic Addiction Risk Severity (GARS) [measures the presence of risk alleles associated with RDS]; as well as their psychosocial traits (questionnaires). BMI changes and sociodemographic data were abstracted from Electronic Health Records. Results: Subjects showed ∆BMI (M = 10.0 ± 1.05 kg/m2) and a mean % excess weight loss (56 ± 13.8%). In addition, 76% of subjects had GARS scores above seven. The homozygote risk alleles for MAO (rs768062321) and DRD1 (rs4532) showed a 38% and 47% prevalence among the subjects. Of the 11 risk alleles identified by GARS, the DRD4 risk allele (rs1800955), was significantly correlated with change in weight and BMI six months post-surgery. We identified correlations with individual risk alleles and psychosocial trait scores. The COMT risk allele (rs4680) showed a negative correlation with EEI scores (r = −0.4983, p < 0.05) and PSQI scores (r = −0.5482, p < 0.05). The GABRB3 risk allele (rs764926719) correlated positively with EEI (r = 0.6161, p < 0.01) and FCQ scores (r = 0.6373, p < 0.01). The OPRM1 risk allele showed a positive correlation with the DERS score (r = 0.5228, p < 0.05). We also identified correlations between DERS and BMI change (r = 0.61; p < 0.01). Conclusions: These data support the potential benefit of a personalized medicinal approach inclusive of genetic testing and psychosocial trait questionnaires when counselling patients with obesity considering bariatric surgery. Future research will explore epigenetic factors that contribute to outcomes of bariatric surgery.