bluuebunny/crossref_metadata_2025 huggingface dataset:
Membrane Cholesterol Inhibits Progesterone-Mediated Sperm Function through the Possible Involvement of ABHD2
Authors: De Toni Luca , Cosci Ilaria , Sabovic Iva , Di Nisio Andrea , Guidolin Diego , Pedrucci Federica , Finocchi Federica , Dall’Acqua Stefano , Foresta Carlo , Ferlin Alberto , Garolla Andrea
Published: May 2023

Abhydrolase domain containing 2-acylglycerol lipase (ABHD2) was recently claimed as the membrane receptor of progesterone (P4) in sperm cells, mediating cell processes such as sperm chemotaxis and acrosome reaction. Here, we investigated the role of membrane cholesterol (Chol) on ABHD2-mediated human sperm chemotaxis. Human sperm cells were obtained from twelve normozoospemic healthy donors. ABHD2–Chol interaction was modelled by computational molecular-modelling (MM). Sperm membrane Chol content was depleted by incubating cells with cyclodextrin (CD) or augmented by the incubation with the complex between CD and Chol (CD:Chol). Cell Chol levels were quantified by liquid chromatography-mass spectrometry. Sperm migration upon P4 gradient was evaluated through the accumulation assay in a specific migration device. Motility parameters were evaluated by sperm class analyzer, whilst intracellular calcium concentration, acrosome reaction and mitochondrial membrane potential were evaluated with calcium orange, FITC-conjugated anti-CD46 antibody and JC-1 fluorescent probes, respectively. MM analysis showed the possible stable binding Chol to ABHD2, resulting in to major impact on the protein backbone flexibility. The treatment with CD was associated with a dose-dependent increase in sperm migration in a 160 nM P4 gradient, together with increase in sperm motility parameters and levels of acrosome reaction. The treatment with CD:Chol was associated with essentially opposite effects. Chol was, thus, suggested to inhibit P4-mediated sperm function through the possible inhibition of ABHD2.
Human Monocytes Are Suitable Carriers for the Delivery of Oncolytic Herpes Simplex Virus Type 1 In Vitro and in a Chicken Embryo Chorioallantoic Membrane Model of Cancer
Authors: Reale Alberto , Krutzke Lea , Cadamuro Massimiliano , Vitiello Adriana , von Einem Jens , Kochanek Stefan , Palù Giorgio , Parolin Cristina , Calistri Arianna
Published: May 2023

Oncolytic viruses (OVs) are promising therapeutics for tumors with a poor prognosis. An OV based on herpes simplex virus type 1 (oHSV-1), talimogene laherparepvec (T-VEC), has been recently approved by the Food and Drug Administration (FDA) and by the European Medicines Agency (EMA) for the treatment of unresectable melanoma. T-VEC, like most OVs, is administered via intratumoral injection, underlining the unresolved problem of the systemic delivery of the oncolytic agent for the treatment of metastases and deep-seated tumors. To address this drawback, cells with a tropism for tumors can be loaded ex vivo with OVs and used as carriers for systemic oncolytic virotherapy. Here, we evaluated human monocytes as carrier cells for a prototype oHSV-1 with a similar genetic backbone as T-VEC. Many tumors specifically recruit monocytes from the bloodstream, and autologous monocytes can be obtained from peripheral blood. We demonstrate here that oHSV-1-loaded primary human monocytes migrated in vitro towards epithelial cancer cells of different origin. Moreover, human monocytic leukemia cells selectively delivered oHSV-1 to human head-and-neck xenograft tumors grown on the chorioallantoic membrane (CAM) of fertilized chicken eggs after intravascular injection. Thus, our work shows that monocytes are promising carriers for the delivery of oHSV-1s in vivo, deserving further investigation in animal models.
625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP Production
Authors: Fu Wenqi , Im Yeong-Gwan , Kim Byunggook , Kim Ok-Su , Yang Ying , Song Jianan , Liu Danyang , Zhu Siyu , Kang Jae-Seok , Kim Okjoon
Published: May 2023

Osteoblasts must acquire a considerable capacity for folding unfolded and misfolded proteins (MPs) to produce large amounts of extracellular matrix proteins and maintain bone homeostasis. MP accumulation contributes to cellular apoptosis and bone disorders. Photobiomodulation therapy has been used to treat bone diseases, but the effects of decreasing MPs with photobiomodulation remain unclear. In this study, we explored the efficacy of 625 nm light-emitting diode irradiation (LEDI) to reduce MPs in tunicamycin (TM) induced-MC3T3-E1 cells. Binding immunoglobulin protein (BiP), an adenosine triphosphate (ATP)-dependent chaperone, is used to evaluate the capacity of folding MPs. The results revealed that pretreatment with 625 nm LEDI (Pre-IR) induced reactive oxygen species (ROS) production, leading to the increased chaperone BiP through the inositol-requiring enzyme 1 (IRE1)/X-box binding protein 1s (XBP-1s) pathway, and then restoration of collagen type I (COL-I) and osteopontin (OPN) expression relieving cell apoptosis. Furthermore, the translocation of BiP into the endoplasmic reticulum (ER) lumen might be followed by a high level of ATP production. Taken together, these results suggest that Pre-IR could be beneficial to prevent MP accumulation through ROS and ATP in TM-induced MC3T3-E1cells.
Deregulated Expression of IL-37 in Patients with Bladder Urothelial Cancer: The Diagnostic Potential of the IL-37e Isoform
Authors: Papasavva Maria , Amvrosiou Styliana , Pilala Katerina-Marina , Soureas Konstantinos , Christodoulou Panayiota , Ji Yuan , Stravodimos Konstantinos , Xu Damo , Scorilas Andreas , Avgeris Margaritis , Christodoulou Maria-Ioanna
Published: May 2023

Cellular and molecular immune components play a crucial role in the development and perpetuation of human malignancies, shaping anti-tumor responses. A novel immune regulator is interleukin-37 (IL-37), already shown to be involved in the inflammation associated with the pathophysiology of many human disorders, including cancer. The interplay between tumor and immune cells is of great importance, especially for highly immunogenic tumors such as bladder urothelial carcinoma (BLCA). This study aimed to investigate the potential of IL-37 and its receptor SIGIRR (single immunoglobulin IL-1-related receptor) to serve as prognostic and/or diagnostic markers in patients with BLCA. To this end, a series of bioinformatics tools processing -omics datasets and specifically designed qPCR assays on human BLCA tumors and cancer cell lines were utilized. Bioinformatics analysis revealed that IL-37 levels correlate with BLCA tumor development and are higher in patients with longer overall survival. Furthermore, mutations on SIGIRR are associated with enhanced infiltration of the tumor by regulatory T cells and dendritic cells. Based on the qPCR validation experiments, BLCA epithelial cells express the IL-37c and IL-37e isoforms, while the latter is the predominant variant detected in tumor biopsies, also associated with higher grade and the non-muscle-invasive type. This is the first time, to the best of our knowledge, that IL-37 and SIGIRR levels have been assessed in BLCA tumor lesions, and associations with pathological and survival parameters are described, while a transcript variant-specific signature is indicated to have a diagnostic potential. These data strongly indicate the need for further investigation of the involvement of this cytokine and interconnected molecules in the pathophysiology of the disease and its prospective as a therapeutic target and biomarker for BLCA.
Effects of Cobra Cardiotoxins on Intracellular Calcium and the Contracture of Rat Cardiomyocytes Depend on Their Structural Types
Authors: Averin Alexey S. , Berezhnov Alexey V. , Pimenov Oleg Y. , Galimova Miliausha H. , Starkov Vladislav G. , Tsetlin Victor I. , Utkin Yuri N.
Published: May 2023

Cardiotoxins (CaTx) of the three-finger toxin family are one of the main components of cobra venoms. Depending on the structure of the N-terminal or the central polypeptide loop, they are classified into either group I and II or P- and S-types, respectively, and toxins of different groups or types interact with lipid membranes variably. While their main target in the organism is the cardiovascular system, there is no data on the effects of CaTxs from different groups or types on cardiomyocytes. To evaluate these effects, a fluorescence measurement of intracellular Ca2+ concentration and an assessment of the rat cardiomyocytes’ shape were used. The obtained results showed that CaTxs of group I containing two adjacent proline residues in the N-terminal loop were less toxic to cardiomyocytes than group II toxins and that CaTxs of S-type were less active than P-type ones. The highest activity was observed for Naja oxiana cobra cardiotoxin 2, which is of P-type and belongs to group II. For the first time, the effects of CaTxs of different groups and types on the cardiomyocytes were studied, and the data obtained showed that the CaTx toxicity to cardiomyocytes depends on the structures both of the N-terminal and central polypeptide loops.
QTL Mapping and Transcriptome Analysis Reveal Candidate Genes Regulating Seed Color in Brassica napus
Authors: Liu Fangying , Chen Hao , Yang Liu , You Liang , Ju Jianye , Yang Shujie , Wang Xiaolin , Liu Zhongsong
Published: May 2023

Yellow seeds are desirable in rapeseed breeding because of their higher oil content and better nutritional quality than black seeds. However, the underlying genes and formation mechanism of yellow seeds remain unclear. Here, a novel yellow-seeded rapeseed line (Huangaizao, HAZ) was crossed with a black-seeded rapeseed line (Zhongshuang11, ZS11) to construct a mapping population of 196 F2 individuals, based on which, a high-density genetic linkage map was constructed. This map, comprising 4174 bin markers, was 1618.33 cM in length and had an average distance of 0.39 cM between its adjacent markers. To assess the seed color of the F2 population, three methods (imaging, spectrophotometry, and visual scoring) were used and a common major quantitative trait locus (QTL) on chromosome A09, explaining 10.91–21.83% of the phenotypic variance, was detected. Another minor QTL, accounting for 6.19–6.69% of the phenotypic variance, was detected on chromosome C03, only by means of imaging and spectrophotometry. Furthermore, a dynamic analysis of the differential expressions between the parental lines showed that flavonoid biosynthesis-related genes were down-regulated in the yellow seed coats at 25 and 35 days after flowering. A coexpression network between the differentially expressed genes identified 17 candidate genes for the QTL intervals, including a flavonoid structure gene, novel4557 (BnaC03.TT4), and two transcription factor genes, namely, BnaA09G0616800ZS (BnaA09.NFYA8) and BnaC03G0060200ZS (BnaC03.NAC083), that may regulate flavonoid biosynthesis. Our study lays a foundation for further identifying the genes responsible for and understanding the regulatory mechanism of yellow seed formation in Brassica napus.
Exercise Training Attenuates Acute β-Adrenergic Receptor Activation-Induced Cardiac Inflammation via the Activation of AMP-Activated Protein Kinase
Authors: Zhang Mi , Alemasi Akehu , Zhao Mingming , Xu Wenli , Zhang Youyi , Gao Wei , Yu Haiyi , Xiao Han
Published: May 2023

Exercise has proven cardiac benefits, but the underlying mechanisms of exercise that protect the heart from acute sympathetic stress injuries remain unknown. In this study, adult C57BL/6J mice and their AMP-activated protein kinase α2 knockout (AMPKα2−/−) littermates were either subjected to 6 weeks of exercise training or housed under sedentary conditions and then treated with or without a single subcutaneous injection of the β-adrenergic receptor (β-AR) agonist isoprenaline (ISO). We investigated the differences in the protective effects of exercise training on ISO-induced cardiac inflammation in wild-type (WT) and AMPKα2−/− mice using histology, enzyme-linked immunosorbent assay (ELISA) and Western blotting analyses. The results indicated that exercise training alleviated ISO-induced cardiac macrophage infiltration, chemokines and the expression of proinflammatory cytokines in wild-type mice. A mechanism study showed that exercise training attenuated the ISO-induced production of reactive oxygen species (ROS) and the activation of NLR Family, pyrin domain-containing 3 (NLRP3) inflammasomes. In cardiomyocytes, the ISO-induced effects on these processes were inhibited by AMP-activated protein kinase (AMPK) activator (metformin) pretreatment and reversed by the AMPK inhibitor (compound C). AMPKα2−/− mice showed more extensive cardiac inflammation following ISO exposure than their wild-type littermates. These results indicated that exercise training could attenuate ISO-induced cardiac inflammation by inhibiting the ROS-NLRP3 inflammasome pathway in an AMPK-dependent manner. Our findings suggested the identification of a novel mechanism for the cardioprotective effects of exercise.
Comparative Characterization of Iron and Silver Nanoparticles: Extract-Stabilized and Classical Synthesis Methods
Authors: Akhatova Farida , Konnova Svetlana , Kryuchkova Marina , Batasheva Svetlana , Mazurova Kristina , Vikulina Anna , Volodkin Dmitry , Rozhina Elvira
Published: May 2023

Synthesis of silver nanoparticles using extracts from plants is an advantageous technological alternative to the traditional colloidal synthesis due to its simplicity, low cost, and the inclusion of environmentally friendly processes to obtain a new generation of antimicrobial compounds. The work describes the production of silver and iron nanoparticles using sphagnum extract as well as traditional synthesis. Dynamic light scattering (DLS) and laser doppler velocimetry methods, UV-visible spectroscopy, transmission electron microscopy (TEM) combined with energy dispersive X-ray spectroscopy (EDS), atomic force microscopy (AFM), dark-field hyperspectral microscopy, and Fourier-transform infrared spectroscopy (FT-IR) were used to study the structure and properties of synthesized nanoparticles. Our studies demonstrated a high antibacterial activity of the obtained nanoparticles, including the formation of biofilms. Nanoparticles synthesized using sphagnum moss extracts likely have high potential for further research.
Influence of Polyphenols on Adipose Tissue: Sirtuins as Pivotal Players in the Browning Process
Authors: Flori Lorenzo , Piragine Eugenia , Spezzini Jacopo , Citi Valentina , Calderone Vincenzo , Martelli Alma
Published: May 2023

Adipose tissue (AT) can be classified into two different types: (i) white adipose tissue (WAT), which represents the largest amount of total AT, and has the main function of storing fatty acids for energy needs and (ii) brown adipose tissue (BAT), rich in mitochondria and specialized in thermogenesis. Many exogenous stimuli, e.g., cold, exercise or pharmacological/nutraceutical tools, promote the phenotypic change of WAT to a beige phenotype (BeAT), with intermediate characteristics between BAT and WAT; this process is called “browning”. The modulation of AT differentiation towards WAT or BAT, and the phenotypic switch to BeAT, seem to be crucial steps to limit weight gain. Polyphenols are emerging as compounds able to induce browning and thermogenesis processes, potentially via activation of sirtuins. SIRT1 (the most investigated sirtuin) activates a factor involved in mitochondrial biogenesis, peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), which, through peroxisome proliferator-activated receptor γ (PPAR-γ) modulation, induces typical genes of BAT and inhibits genes of WAT during the transdifferentiation process in white adipocytes. This review article aims to summarize the current evidence, from pre-clinical studies to clinical trials, on the ability of polyphenols to promote the browning process, with a specific focus on the potential role of sirtuins in the pharmacological/nutraceutical effects of natural compounds.
Conformation-Specific Association of Prion Protein Amyloid Aggregates with Tau Protein Monomers
Authors: Ziaunys Mantas , Mikalauskaite Kamile , Krasauskas Lukas , Smirnovas Vytautas
Published: May 2023

Protein aggregation into amyloid fibrils is associated with several amyloidoses, including neurodegenerative Alzheimer’s and Parkinson’s diseases. Despite years of research and numerous studies, the process is still not fully understood, which significantly impedes the search for cures of amyloid-related disorders. Recently, there has been an increase in reports of amyloidogenic protein cross-interactions during the fibril formation process, which further complicates the already intricate process of amyloid aggregation. One of these reports displayed an interaction involving Tau and prion proteins, which prompted a need for further investigation into the matter. In this work, we generated five populations of conformationally distinct prion protein amyloid fibrils and examined their interaction with Tau proteins. We observed that there was a conformation-specific association between Tau monomers and prion protein fibrils, which increased the aggregate self-association and amyloidophilic dye binding capacity. We also determined that the interaction did not induce the formation of Tau protein amyloid aggregates, but rather caused their electrostatic adsorption to the prion protein fibril surface.
Crosstalk of Immune Cells and Platelets in an Ovarian Cancer Microenvironment and Their Prognostic Significance
Authors: Pankowska Katarzyna Aneta , Będkowska Grażyna Ewa , Chociej-Stypułkowska Joanna , Rusak Małgorzata , Dąbrowska Milena , Osada Joanna
Published: May 2023

Ovarian cancer (OC) is one of the deadliest gynecological cancers, largely due to the fast development of metastasis and drug resistance. The immune system is a critical component of the OC tumor microenvironment (TME) and immune cells such as T cells, NK cells, and dendritic cells (DC) play a key role in anti-tumor immunity. However, OC tumor cells are well known for evading immune surveillance by modulating the immune response through various mechanisms. Recruiting immune-suppressive cells such as regulatory T cells (Treg cells), macrophages, or myeloid-derived suppressor cells (MDSC) inhibit the anti-tumor immune response and promote the development and progression of OC. Platelets are also involved in immune evasion by interaction with tumor cells or through the secretion of a variety of growth factors and cytokines to promote tumor growth and angiogenesis. In this review, we discuss the role and contribution of immune cells and platelets in TME. Furthermore, we discuss their potential prognostic significance to help in the early detection of OC and to predict disease outcome.
Faujasite-Type Zeolite Obtained from Ecuadorian Clay as a Support of ZnTiO3/TiO2 NPs for Cyanide Removal in Aqueous Solutions
Authors: Jaramillo-Fierro Ximena , Alvarado Hipatia , Montesdeoca Fernando , Valarezo Eduardo
Published: May 2023

In this study, zeolites prepared by the hydrothermal method from Ecuadorian clay were combined with the precursor clay and with the semiconductor ZnTiO3/TiO2 prepared by the sol-gel method to adsorb and photodegrade cyanide species from aqueous solutions. These compounds were characterized by X-ray powder diffraction, X-ray fluorescence, scanning electron microscopy, energy-dispersive X-rays, point of zero charge, and specific surface area. The adsorption characteristics of the compounds were measured using batch adsorption experiments as a function of pH, initial concentration, temperature, and contact time. The Langmuir isotherm model and the pseudo-second-order model fit the adsorption process better. The equilibrium state in the reaction systems at pH = 7 was reached around 130 and 60 min in the adsorption and photodegradation experiments, respectively. The maximum cyanide adsorption value (73.37 mg g−1) was obtained with the ZC compound (zeolite + clay), and the maximum cyanide photodegradation capacity (90.7%) under UV light was obtained with the TC compound (ZnTiO3/TiO2 + clay). Finally, the reuse of the compounds in five consecutive treatment cycles was determined. The results reflect that the compounds synthesized and adapted to the extruded form could potentially be used for the removal of cyanide from wastewater.
Cinnamaldehyde Restores Ceftriaxone Susceptibility against Multidrug-Resistant Salmonella
Authors: Yin Lizi , Gou Yuhong , Dai Yuyun , Wang Tao , Gu Kexin , Tang Ting , Hussain Sajjad , Huang Xiaoli , He Changliang , Liang Xiaoxia , Shu Gang , Xu Funeng , Ouyang Ping
Published: May 2023

In recent years, infections caused by multidrug-resistant (MDR) bacteria have greatly threatened human health and imposed a burden on global public health. To overcome this crisis, there is an urgent need to seek effective alternatives to single antibiotic therapy to circumvent drug resistance and prevent MDR bacteria. According to previous reports, cinnamaldehyde exerts antibacterial activity against drug-resistant Salmonella spp. This study was conducted to investigate whether cinnamaldehyde has a synergistic effect on antibiotics when used in combination, we found that cinnamaldehyde enhanced the antibacterial activity of ceftriaxone sodium against MDR Salmonella in vitro by significantly reduced the expression of extended-spectrum beta-lactamase, inhibiting the development of drug resistance under ceftriaxone selective pressure in vitro, damaging the cell membrane, and affecting its basic metabolism. In addition, it restored the activity of ceftriaxone sodium against MDR Salmonella in vivo and inhibited peritonitis caused by ceftriaxone resistant strain of Salmonella in mice. Collectively, these results revealed that cinnamaldehyde can be used as a novel ceftriaxone adjuvant to prevent and treat infections caused by MDR Salmonella, mitigating the possibility of producing further mutant strains.
Investigations on Primary Cilia of Nthy-ori 3-1 Cells upon Cysteine Cathepsin Inhibition or Thyrotropin Stimulation
Authors: Doğru Alara Gaye , Rehders Maren , Brix Klaudia
Published: May 2023

In the thyroid gland, cysteine cathepsins are secreted upon thyrotropin stimulation for thyroglobulin processing, and they are present at the primary cilia of thyroid epithelial cells. Treatment with protease inhibitors resulted in the loss of cilia from rodent thyrocytes and caused redistribution of the thyroid co-regulating G protein-coupled receptor Taar1 to the endoplasmic reticulum. These findings suggest that ciliary cysteine cathepsins are important to maintain sensory and signaling properties for the proper regulation and homeostasis of thyroid follicles. Therefore, it is important to better understand how cilia structure and frequencies are maintained in human thyroid epithelial cells. Hence, we aimed to investigate the potential role of cysteine cathepsins for the maintenance of primary cilia in the normal human Nthy-ori 3-1 thyroid cell line. This was approached by determining cilia lengths and frequencies in cysteine peptidase inhibition conditions in Nthy-ori 3-1 cell cultures. Cilia lengths were shortened upon 5 h of cysteine peptidase inhibition with cell-impermeable E64. Likewise, cilia lengths and frequencies were decreased upon additional overnight treatment with the cysteine peptidase-targeting, activity-based probe DCG-04. The results suggest that cysteine cathepsin activity is required for the maintenance of the cellular protrusions not only in rodents, but also in human thyrocytes. Hence, thyrotropin stimulation was used to simulate physiological conditions that eventually lead to cathepsin-mediated thyroglobulin proteolysis, which is initiated in the thyroid follicle lumen. Immunoblotting revealed that thyrotropin stimulation conditions result in the secretion of little procathepsin L and some pro- and mature cathepsin S but no cathepsin B from the human Nthy-ori 3-1 cells. Unexpectedly, however, 24 h incubation periods with thyrotropin shortened the cilia although higher amounts of cysteine cathepsins were present in the conditioned media. These data point to the necessity of further studies to delineate which of the cysteine cathepsins plays the most prominent role in cilia shortening and/or elongation. Collectively, the results of our study provide corroboration for the hypothesis of thyroid autoregulation by local mechanisms that our group previously proposed.
Repeated Rounds of Gonadotropin Stimulation Induce Imbalance in the Antioxidant Machinery and Activation of Pro-Survival Proteins in Mouse Oviducts
Authors: Di Nisio Valentina , Antonouli Sevastiani , Colafarina Sabrina , Zarivi Osvaldo , Rossi Gianna , Cecconi Sandra , Poma Anna Maria Giuseppina
Published: May 2023

Controlled ovarian stimulation (COS) through gonadotropin administration has become a common procedure in assisted reproductive technologies. COS’s drawback is the formation of an unbalanced hormonal and molecular environment that could alter several cellular mechanisms. On this basis, we detected the presence of mitochondrial DNA (mtDNA) fragmentation, antioxidant enzymes (catalase; superoxide dismutases 1 and 2, SOD-1 and -2; glutathione peroxidase 1, GPx1) and apoptotic (Bcl-2-associated X protein, Bax; cleaved caspases 3 and 7; phosphorylated (p)-heat shock protein 27, p-HSP27) and cell-cycle-related proteins (p-p38 mitogen-activated protein kinase, p-p38 MAPK; p-MAPK activated protein kinase 2, p-MAPKAPK2; p-stress-activated protein kinase/Jun amino-terminal kinase, p-SAPK/JNK; p-c-Jun) in the oviducts of unstimulated (Ctr) and repeatedly hyperstimulated (eight rounds, 8R) mice. While all the antioxidant enzymes were overexpressed after 8R of stimulation, mtDNA fragmentation decreased in the 8R group, denoting a present yet controlled imbalance in the antioxidant machinery. Apoptotic proteins were not overexpressed, except for a sharp increase in the inflammatory-related cleaved caspase 7, accompanied by a significant decrease in p-HSP27 content. On the other hand, the number of proteins involved in pro-survival mechanisms, such as p-p38 MAPK, p-SAPK/JNK and p-c-Jun, increased almost 50% in the 8R group. Altogether, the present results demonstrate that repeated stimulations cause the activation of the antioxidant machinery in mouse oviducts; however, this is not sufficient to induce apoptosis, and is efficiently counterbalanced by activation of pro-survival proteins.
The Psychosocial Impact of Congenital Cytomegalovirus on Caregivers and Families: Lived Experiences and Review of the Literature
Authors: Zappas Michelle P. , Devereaux Amanda , Pesch Megan H.
Published: May 2023

Caring for a child with congenital cytomegalovirus (cCMV) can be costly for families, not only in terms of out-of-pocket expenses, but also in terms of caregiver time, relationships, career trajectories, and mental health. These additional burdens are sometimes referred to as “spillover effects”. As parents of children with cCMV, we, the authors of this article, discuss the impact that cCMV has had on our families. While multiple studies have reported on the epidemiology, prevention, screening, diagnosis, and management of cCMV, there has been minimal research regarding the possible impact on the family unit. In this narrative review, we discuss the various areas of the lives of families and caregivers that may be impacted by raising a child with cCMV. Whether children are minimally or severely affected by the sequelae of cCMV, they and their families merit the progression of awareness of the virus and governmental policies to help end cCMV. As the existing cCMV-specific literature is limited, we correlate studies of other childhood disabilities and find the mutuality experienced by families affected by cCMV.
The Landscape of the DNA Transposons in the Genome of the Horezu_LaPeri Strain of Drosophila melanogaster
Authors: Bologa Alexandru Marian , Stoica Ileana , Constantin Nicoleta Denisa , Ecovoiu Alexandru Al.
Published: May 2023

Natural transposons (NTs) represent mobile DNA sequences found in both prokaryotic and eukaryotic genomes. Drosophila melanogaster (the fruit fly) is a eukaryotic model organism with NTs standing for about 20% of its genome and has contributed significantly to the understanding of various aspects of transposon biology. Our study describes an accurate approach designed to map class II transposons (DNA transposons) in the genome of the Horezu_LaPeri fruit fly strain, consecutive to Oxford Nanopore Technology sequencing. A whole genome bioinformatics analysis was conducted using Genome ARTIST_v2, LoRTE and RepeatMasker tools to identify DNA transposons insertions. Then, a gene ontology enrichment analysis was performed in order to evaluate the potential adaptive role of some DNA transposons insertions. Herein, we describe DNA transposon insertions specific for the Horezu_LaPeri genome and a predictive functional analysis of some insertional alleles. The PCR validation of P-element insertions specific for this fruit fly strain, along with a putative consensus sequence for the KP element, is also reported. Overall, the genome of the Horezu_LaPeri strain contains several insertions of DNA transposons associated with genes known to be involved in adaptive processes. For some of these genes, insertional alleles obtained via mobilization of the artificial transposons were previously reported. This is a very alluring aspect, as it suggests that insertional mutagenesis experiments conducting adaptive predictions for laboratory strains may be confirmed by mirroring insertions which are expected to be found at least in some natural fruit fly strains.
Feeding and Amines Stimulate the Growth of the Salivary Gland following Short-Term Starvation in the Black Field Cricket, Teleogryllus commodus
Authors: Othman Nurul Wahida , Barron Andrew B. , Cooper Paul D.
Published: May 2023

The salivary gland of the black field cricket, Teleogryllus commodus Walker changed size between being starved and fed. Crickets without access to food for 72 h showed a reduction in both wet and dry mass of the glands compared with the glands from continuously fed animals at 72 h. Glands returned to size following ingestion within 10 min. Salivary glands of starved crickets (72 h) were incubated in saline containing either serotonin (5-HT) or dopamine (DA). Glands increased to pre-starvation size after 1 h incubation in situ with either 10−4 moles L−1 5-HT or 10−4 moles L−1 DA, although lower concentrations (10−5 moles L−1) did not affect gland size. From immunohistochemistry, amines appeared to shift from zymogen cells during starvation to parietal cells following feeding. High-performance liquid chromatography showed that serotonin concentration is higher than dopamine in the salivary gland removed from starved and fed crickets, but the quantity of these compounds was not dependent upon feeding state; the amine quantities increased as gland size increased. Further work is necessary to determine what might be the stimulus for gland growth and if dopamine and serotonin play a role in the stimulation of salivary gland growth after a period of starvation.
A Review of Risk Scores within Upper Gastrointestinal Bleeding
Authors: Orpen-Palmer Josh , Stanley Adrian J.
Published: May 2023

Upper gastrointestinal bleeding is a common medical emergency. Thorough initial assessment and appropriate resuscitation are essential to stabilise the patient. Risk scores provide an important tool to discriminate between lower- and higher-risk patients. Very low-risk patients can be safely discharged for out-patient management, while higher-risk patients can receive appropriate in-patient care. The Glasgow Blatchford Score, with a score of 0–1, performs best in the identification of very low-risk patients who will not require hospital based intervention or die, and is recommended by most guidelines to facilitate safe out-patient management. The performance of risk scores in the identification of specific adverse events to define high-risk patients is less accurate, with no individual score performing consistently well. Ongoing developments in the use of machine learning models and artificial intelligence in predicting poor outcomes in UGIB appear promising and will likely form the basis of dynamic risk assessment in the future.
Fractional CO2 Laser Treatment Is Safe and Effective for the Management of Genitourinary Syndrome of Menopause in Korean Women
Authors: Chung Youn-Jee , Shim Suhyun , Kim Sejin , Cha Jimin , Song Jae-Yen , Kim Min Jeong , Kim Mee-Ran
Published: May 2023

This study evaluates the efficacy and safety of fractional CO2 lasers for treating genitourinary syndrome of menopause (GSM) in Korean women. The patients received three laser applications at an interval of 4 weeks each. The severity of GSM symptoms was assessed using a visual analog scale (VAS) at baseline and at every visit. The objective scale was measured using the vaginal health index score (VHIS) and Vaginal Maturation Index (VMI) after completion of the laser procedure. During each procedure, the patients’ pain in the VAS score was recorded. In the last visit, patients evaluated their satisfaction with the laser therapy using a 5-point Likert scale. Thirty women completed all the study protocols. After two sessions of laser therapy, some GSM symptoms (vaginal dryness and urgency) and VHIS improved significantly. After completion of the treatment, all GSM symptoms improved (p < 0.05), and the VHIS further increased significantly (VHIS at baseline, 8.86 ± 3.2 vs. V3, 16.83 ± 3.15, p < 0.001). The average satisfaction was 4.3. This study shows that fractional CO2 laser treatment is effective and safe for Korean women with GSM. Further studies are needed to confirm these results and assess the long-term effects of laser therapy.
Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Patients with ST-Elevation Myocardial Infarction and Its Association with Acute Kidney Injury and Mortality
Authors: Højagergaard Mathias Alexander , Beske Rasmus Paulin , Hassager Christian , Holmvang Lene , Jensen Lisette Okkels , Shacham Yacov , Meyer Martin Abild Stengaard , Moeller Jacob Eifer , Helgestad Ole Kristian Lerche , Mark Peter Dall , Møgelvang Rasmus , Frydland Martin
Published: May 2023

Neutrophil gelatinase-associated lipocalin (NGAL) is an inflammatory biomarker related to acute kidney injury (AKI). Including 1892 consecutive patients with ST-elevation myocardial infarction (STEMI), in which NGAL was measured in 1624 (86%) on admission and in a consecutive subgroup at 6–12 h (n = 163) and 12–24 h (n = 222) after admission, this study aimed to evaluate the prognostic value of NGAL in predicting AKI and mortality. Patients were stratified based on whether their admission NGAL plasma concentration was greater than or equal to/less than the median. The primary endpoint was a composite of the first occurrence of AKI or all-cause death within 30 days. AKI was classified by the maximal plasma creatinine increase from baseline during index admission as KDIGO1 (<200% increase) or KDIGO23 (≥200% increase) according to the Kidney Disease Improving Global Outcomes (KDIGO) system. Admission NGAL > the median was independently associated with a higher risk of severe AKI (KDIGO2-3) and 30-day all-cause mortality when adjusted for age, admission systolic blood pressure and high-sensitivity C-reactive protein, left-ventricular ejection fraction, known kidney dysfunction, and cardiogenic shock with an odds ratio (95% confidence interval) of 2.26 (1.18–4.51), p = 0.014. Finally, we observed increasing predictive values in a subgroup during the first day of hospitalization suggesting that assessment of NGAL should be delayed for optimal prognostic purposes.
Effects of Polishing and Artificial Aging on Mechanical Properties of Dental LT Clear® Resin
Authors: Paradowska-Stolarz Anna , Wezgowiec Joanna , Malysa Andrzej , Wieckiewicz Mieszko
Published: May 2023

Three-dimensional printing has become incorporated into various aspects of everyday life, including dentistry. Novel materials are being introduced rapidly. One such material is Dental LT Clear by Formlabs, a resin used for manufacturing occlusal splints, aligners, and orthodontic retainers. In this study, a total of 240 specimens, comprising two shapes (dumbbell and rectangular), were evaluated through compression and tensile tests. The compression tests revealed that the specimens were neither polished nor aged. However, after polishing, the compression modulus values decreased significantly. Specifically, the unpolished and nonaged specimens measured 0.87 ± 0.02, whereas the polished group measured 0.086 ± 0.03. The results were significantly affected by artificial aging. The polished group measured 0.73 ± 0.05, while the unpolished group measured 0.73 ± 0.03. In contrast, the tensile test proved that the specimens showed the highest resistance when the polishing was applied. The artificial aging influenced the tensile test and reduced the force needed to damage the specimens. The tensile modulus had the highest value when polishing was applied (3.00 ± 0.11). The conclusions drawn from these findings are as follows: 1. Polishing does not change the properties of the examined resin. 2. Artificial aging reduces resistance in both compression and tensile tests. 3. Polishing reduces the damage to the specimens in the aging process.
Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm Formation
Authors: Seo Bo Yun , Son KeunBaDa , Son Young-Tak , Dahal Ram Hari , Kim Shukho , Kim Jungmin , Hwang JunHo , Kwon Sung-Min , Lee Jae-Mok , Lee Kyu-Bok , Kim Jin-Wook
Published: May 2023

This study aimed to evaluate the impact of different surface treatments (machined; sandblasted, large grit, and acid-etched (SLA); hydrophilic; and hydrophobic) on dental titanium (Ti) implant surface morphology, roughness, and biofilm formation. Four groups of Ti disks were prepared using distinct surface treatments, including femtosecond and nanosecond lasers for hydrophilic and hydrophobic treatments. Surface morphology, wettability, and roughness were assessed. Biofilm formation was evaluated by counting the colonies of Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), and Prevotella intermedia (Pi) at 48 and 72 h. Statistical analysis was conducted to compare the groups using the Kruskal–Wallis H test and the Wilcoxon signed-rank test (α = 0.05). The analysis revealed that the hydrophobic group had the highest surface contact angle and roughness (p < 0.05), whereas the machined group had significantly higher bacterial counts across all biofilms (p < 0.05). At 48 h, the lowest bacterial counts were observed in the SLA group for Aa and the SLA and hydrophobic groups for Pg and Pi. At 72 h, low bacterial counts were observed in the SLA, hydrophilic, and hydrophobic groups. The results indicate that various surface treatments affect implant surface properties, with the hydrophobic surface using femtosecond laser treatment exerting a particularly inhibitory effect on initial biofilm growth (Pg and Pi).
A Fault Diagnosis Method for the Autonomous Underwater Vehicle via Meta-Self-Attention Multi-Scale CNN
Authors: Chen Yimin , Wang Yazhou , Yu Yang , Wang Jiarun , Gao Jian
Published: May 2023

Autonomous underwater vehicles (AUVs) are an important equipment for ocean investigation. Actuator fault diagnosis is essential to ensure the sailing safety of AUVs. However, the lack of failure data for training due to unknown ocean environments and unpredictable failure occurrences is challenging for fault diagnosis. In this paper, a meta-self-attention multi-scale convolution neural network (MSAMS–CNN) is proposed for the actuator fault diagnosis of AUVs. Specifically, a two-dimensional spectrogram of the vibration signals obtained by a vibration sensor is used as the neural network’s inputs. The diagnostic model is fitted by executing a subtask-based gradient optimization procedure to generate more general degradation knowledge. A self-attentive multi-scale feature extraction approach is used to utilize both global and local features for learning important parameters autonomously. In addition, a meta-learning method is utilized to train the diagnostic model without a large amount of labeled data, which enhances the generalization ability and allows for cross-task training. Experimental studies with real AUV data collected by vibration sensors are conducted to validate the effectiveness of the MSAMS–CNN. The results show that the proposed method can diagnose the rudder and thruster faults of AUVs in the cases of few-shot diagnosis.
Patterns, Trends, and Causes of Vegetation Change in the Three Rivers Headwaters Region
Authors: Zhang Xiongyi , Ning Jia
Published: May 2023

The Three Rivers Headwaters Region (TRHR), situated in the high-altitude region of the Tibetan Plateau, represents a critical ecological security barrier for both China and Southeast Asia. In addition to providing a vital freshwater supply for China, it is also one of the most biodiverse areas in the world. However, the region is characterized by a fragile and sensitive ecosystem, which makes it particularly vulnerable to environmental disturbances. Given the role of vegetation as a reliable indicator of ecosystem changes, investigating the patterns and drivers of vegetation change in the TRHR can provide valuable insights into the state and trajectory of its ecological system. To assess the spatiotemporal dynamics of vegetation changes in TRHR, this study constructed a comprehensive vegetation index (CVI) based on leaf area index (LAI), fractional vegetation cover (FVC), and net primary productivity (NPP). Trend analysis and significance testing were employed to detect and quantify the magnitude and direction of vegetation changes over the past two decades. Moreover, correlation analysis, spatial overlay, and statistical modeling techniques were applied to examine the relative contributions of climate, land use/cover changes, and slope altitude to CVI changes. The results showed the following: (1) the CVI in the TRHR showed an overall increasing trend from 2000 to 2019, with an average annual increase of 0.6%, indicating a positive development trend of vegetation, but with obvious spatial differences. (2) The vegetation in the TRHR has shown a marked increase over the past two decades, with significant increase accounting for 19% of the total area, mainly concentrated in the Yellow River Basin. Conversely, a significant decline in vegetation was observed in 3% of the total area, predominantly in the Yangtze River Basin. (3) Both temperature and precipitation had a positive impact on vegetation restoration in the TRHR, but the area affected by temperature was larger than that affected by precipitation. (4) The land-use regime had a discernible impact on vegetation increase, with the conversion of land use to ecological land promoting vegetation restoration. However, the efficacy of restoration efforts might have varied depending on natural resource endowments in certain regions. (5) With increasing altitude, vegetation conditions initially improved before eventually deteriorating. Conversely, as the slope increased, vegetation experienced an initial improvement before ultimately stabilizing. This study helps to understand the overall changes and driving mechanisms of vegetation in the TRHR and provides a scientific basis and decision-making reference for the dynamic adjustment of vegetation restoration and ecological engineering in the region in the future.
Spinal Cord Organoids to Study Motor Neuron Development and Disease
Authors: Buchner Felix , Dokuzluoglu Zeynep , Grass Tobias , Rodriguez-Muela Natalia
Published: May 2023

Motor neuron diseases (MNDs) are a heterogeneous group of disorders that affect the cranial and/or spinal motor neurons (spMNs), spinal sensory neurons and the muscular system. Although they have been investigated for decades, we still lack a comprehensive understanding of the underlying molecular mechanisms; and therefore, efficacious therapies are scarce. Model organisms and relatively simple two-dimensional cell culture systems have been instrumental in our current knowledge of neuromuscular disease pathology; however, in the recent years, human 3D in vitro models have transformed the disease-modeling landscape. While cerebral organoids have been pursued the most, interest in spinal cord organoids (SCOs) is now also increasing. Pluripotent stem cell (PSC)-based protocols to generate SpC-like structures, sometimes including the adjacent mesoderm and derived skeletal muscle, are constantly being refined and applied to study early human neuromuscular development and disease. In this review, we outline the evolution of human PSC-derived models for generating spMN and recapitulating SpC development. We also discuss how these models have been applied to exploring the basis of human neurodevelopmental and neurodegenerative diseases. Finally, we provide an overview of the main challenges to overcome in order to generate more physiologically relevant human SpC models and propose some exciting new perspectives.
Numerical Modeling of the Dynamic Elastic Modulus of Concrete
Authors: Gidrão Gustavo de Miranda Saleme , Carrazedo Ricardo , Bosse Rúbia Mara , Silvestro Laura , Ribeiro Rodrigo , de Souza Carlos Francisco Pecapedra
Published: May 2023

This article introduces simulations of theoretical material with controlled properties for the evaluation of the effect of key parameters, as volumetric fractions, elastic properties of each phase and transition zone on the effective dynamic elastic modulus. The accuracy level of classical homogenization models was checked regarding the prediction of dynamic elastic modulus. Numerical simulations were performed with the finite element method for evaluations of the natural frequencies and their correlation with Ed through frequency equations. An acoustic test validated the numerical results and obtained the elastic modulus of concretes and mortars at 0.3, 0.5 and 0.7 water–cement ratios. Hirsch calibrated according to the numerical simulation (x = 0.27) exhibited a realistic behavior for concretes of w/c = 0.3 and 0.5, with a 5% error. However, when the water-to-cement ratio (w/c) was set to 0.7, Young’s modulus displayed a resemblance to the Reuss model, akin to the simulated theoretical triphasic materials, considering matrix, coarse aggregate and a transition zone. Hashin-Shtrikman bounds is not perfectly applied to theoretical biphasic materials under dynamic situations.
On the Influence of Heat Input on Ni-WC GMAW Hardfaced Coating Properties
Authors: Pawlik Jan , Bembenek Michał , Góral Tomasz , Cieślik Jacek , Krawczyk Janusz , Łukaszek-Sołek Aneta , Śleboda Tomasz , Frocisz Łukasz
Published: May 2023

Hardfacing is one of the techniques used for part lifecycle elongation. Despite being used for over 100 years, there still is much to discover, as modern metallurgy provides more and more sophisticated alloys, which then have to be studied to find the best technological parameters in order to fully utilize complex material properties. One of the most efficient and versatile hardfacing approaches is Gas Metal Arc Welding technology (GMAW) and its cored-wire equivalent, known as FCAW (Flux-Cored/Cored Arc Welding). In this paper, the authors study the influence of heat input on the geometrical properties and hardness of stringer weld beads fabricated from cored wire consisting of macrocrystalline tungsten carbides in a nickel matrix. The aim is to establish a set of parameters which allow to manufacture wear-resistant overlays with high deposition rates, preserving all possible benefits of this heterogenic material. This study shows, that for a given diameter of the Ni-WC wire, there exists an upper limit of heat input beyond which the tungsten carbide crystals may exhibit undesired segregation at the root.