bluuebunny/crossref_metadata_2025 huggingface dataset:
The effect of fibroblast growth factor 21 on a mouse model of bovine viral diarrhea
Authors: Zhao Dan , Song Yu-Hao , Song Jin-Ming , Shi Kun , Li Jian-Ming , Diao Nai-Chao , Zong Ying , Zeng Fan-Li , Du Rui
Published: March 2023

Previously, we researched that bovine viral diarrhea virus (BVDV) induced a very significant increase in fibroblast growth factor 21 (FGF21) expression in mouse liver and that FGF21 was increased in the peripheral blood of BVD cattle and BVD mice. To determine the role of FGF21 in relieving clinical symptoms and inhibiting the intestinal damage caused by BVDV in BVD development in mice, BALB/c mice were intraperitoneally injected with cytopathic biotype (cp) BVDV-LS01 (isolated and identified by our group) to establish a BVD mouse model. The role of FGF21 in the BVD mouse model was investigated by injecting the mice with FGF21. The animals were divided into control, BVDV challenge, BVDV + FGF21, BVDV + FGF21Ab (anti-FGF21 antibody), and BVDV + IgG (immunoglobulin G) groups. The stool consistency, the degree of bloody diarrhea, histopathological changes, inflammatory cell infiltration, weight loss percentage, and detection of BVDV in the feces of the mice were examined, and the pathological changes and inflammatory cytokine expression were analyzed. The results showed that after BVDV challenge, the average BVD mouse model score of the BVDV mice was 11.6 points. In addition to mild diarrhea and tissue damage, BVDV was detected in the stools of 13 BVDV mice. Only two mice in the control group had scores (both, 1 point each). The comprehensive scoring results demonstrated the successful establishment of the BVD mouse model. FGF21 alleviated the clinical symptoms in the BVD mice and significantly improved weight loss. Furthermore, FGF21 inhibited the BVDV-induced leukocyte, platelet, and lymphocyte reduction while inhibiting the expression of BVDV-induced inflammatory factors. In the BVD mice, FGF21 promoted duodenal epithelial cell proliferation, thereby significantly improving the damage to the cells. In conclusion, FGF21 exerted a good therapeutic effect on the BVD mouse model.
Infectious abortions in small domestic ruminants in the Iberian Peninsula: Optimization of sampling procedures for molecular diagnostics
Authors: Alzuguren Oihane , Domínguez Lara , Chacón Gema , Benito Alfredo A. , Mencía-Ares Oscar
Published: March 2023

Introduction

Infectious abortions have a major impact on small domestic ruminant farms, i.e., sheep and goats, both in terms of profitability and health status. Therefore, rapid and sensitive diagnosis is essential to minimize losses. Currently, molecular techniques, such as qPCR, are routinely used for their diagnosis, which imply the need to manipulate all abortive material, with consequent biosafety risks. Here, we evaluate the frequency of the main abortifacient pathogens in small domestic ruminants in the Iberian Peninsula and also assess an alternative approach for the optimization of sampling for molecular diagnosis.

Results

A total of 392 clinical cases were analyzed from April 2020 to May 2021, evidencing that the main causative agents of abortion detected were Coxiella burnetii (49.0%), Chlamydia abortus (38.3%) and, to a lesser extent, Toxoplasma gondii (10.2%), Salmonella enterica (7.1%) and Campylobacter spp. (6.1%). An uneven distribution of these pathogens was observed between ruminant species, with a higher frequency (p < 0.05) of T. gondii, S. enterica and Campylobacter spp. in sheep than goat abortions, and among geographic areas, highlighting the higher frequency (p < 0.05) of T. gondii and Campylobacter spp. in the north compared to southeastern Spain. The alternative sampling method, consisting on the use of fetal tongues and placental swabs in replacement of the whole fetus and placental tissue, offered a very good agreement with the classical method for all pathogens, except for low concentrations of C. burnetii, which seems to have a doubtful role in abortion when its concentration in the abortifacient material is low.

Conclusions

This study reveals a high frequency of infectious etiology in abortions of small domestic ruminants in the Iberian Peninsula and validates for the first time an alternative sampling method for molecular diagnosis that will help to provide rapid and accurate results while minimizing biosafety risks.
Abdominal Closure With Reinforcing Suture Decreases Incisional Hernia Incidence After CRS/HIPEC
Authors: Wenzelberg Charlotta , Petersson Ulf , Syk Ingvar , Ekberg Olle , Rogmark Peder
Published: March 2023

Background: Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) entails several risk factors for incisional hernia (IH). A few reports available showing incidences between 7% and 17%. At our institution fascia closure has been performed in a 4:1 suture to wound length manner, with a continuous 2-0 polydiaxanone suture (PDS-group) or with a 2-0 polypropylene suture preceded by a reinforced tension line (RTL) suture (RTL-group). Our hypothesis was that these patients might benefit from reinforcing the suture line with a lower IH incidence in this group. The aim was to evaluate the 1-year IH-incidence of the two different closures.

Methods: Patients eligible for inclusion were treated with CRS/HIPEC between 2004 and 2019. IH was diagnosed by scrutinizing CT-scans 1 year ±3 months after surgery. Additional data was retrieved from clinical records and a prospective CRS/HIPEC-database.

Results: Of 193 patients, 129 were included, 82 in the PDS- and 47 in the RTL-group. RTL-patients were 5 years younger, had less blood loss and more frequent postoperative neutropenia. No difference regarding sex, BMI, recent midline incisions, excision of midline scars, peritoneal cancer index score, complications (≥Clavien-Dindo 3b), or chemotherapy. Ten IH (7.8%) were found, 9 (11%) in the PDS- and 1 (2.1%) in the RTL-group (p = 0.071).

Conclusion: An IH incidence of 7.8% in patients undergoing CRS/HIPEC is not higher than after laparotomies in general. The IH incidence in the PDS-group was 11% compared to 2% in the RTL-group. Even though significance was not reached, the difference is clinically relevant, suggesting an advantage with RTL suture.
Concurrent Sub-Region Collaborative Method for Static and Dynamic Coupled Layout Optimization of Stiffened Plates
Authors: Xie Chuan , Yao Weixing , Zhou Danfa , Xue Caijun
Published: March 2023

The main concern of the paper is the concurrent treatment of size and layout variables in the static–dynamic coupled layout optimization of stiffened plates. As compared to size optimization alone, layout optimization is a more challenging task, and the problem will become more difficult and complex if the coupling of statics and dynamics is then considered simultaneously. A concurrent sub-region collaborative (CSRC) optimization strategy is proposed in this paper based on the idea of concurrency in multidisciplinary design optimization. The core idea is to decompose the original complex problem into multiple sub-problems by decoupling twice and maintaining the connection with the original problem by sub-region coordination and consistency constraints. A dynamic driving function is added to the CSRC method to solve the problem of iteration difficulty in the feasible region of the collaborative optimization (CO) method, and the performance of the driving function is illustrated by a numerical test. Furthermore, an adaptive surrogate model (ASM) based on the benchmark sub-region is modeled, which improves the prediction accuracy and optimization speed. Finally, a static–dynamic coupled layout optimization of a metal stiffened plate is performed, and the result indicates that the CSRC method performs well on the given case, and its concurrency property makes it possible to not only enlarge the search space but also reduce a significant “wall-clock time” compared to the sequential strategy.
Parametric Investigation of Canards on a Flying Wing UAV Using the Taguchi Method
Authors: Kapsalis Stavros , Bliamis Chris , Kaparos Pavlos , Panagiotou Pericles , Yakinthos Kyros
Published: March 2023

The current work investigates the effect of canard geometric characteristics on the performance of a lightweight flying wing Unmanned Aerial Vehicle (UAV), capable of both conventional and Vertical Take-Off and Landing (VTOL) flight. The canards are sized as horizontal stabilizers to enhance the UAV’s longitudinal stability and minimize trimming requirements during cruise. Using a Design of Experiments (DOE) approach and, specifically, the Taguchi method, six canards’ design parameters are investigated on three different levels. These parameters are the sweep angle (Λ), aspect ratio (AR), taper ratio (λ), vertical position in relation to the main wing (vpos), incidence angle (ic), and dihedral angle (Γ). An L27 orthogonal array (OA) is used to investigate the influence of these key design parameters using two performance criteria, namely the Lift-to-Drag ratio (L/D) and the pitching moment coefficient (Cm), at cruise conditions (designated as L/Dcruise and |Cm|cruise). The investigation is conducted by using high-fidelity Computational Fluid Dynamics (CFD) methods for each of the 27 configurations defined by the L27 OA, over a range of angles of attack. Based on the CFD results, two distinct combinations are derived for maximum L/Dcruise and minimum |Cm|cruise using the Signal-to-Noise ratio (SNR) analysis. The optimal design parameter combinations for the two performance criteria are A2B1C1D1E1F1 and A2B2C1D2E2F3, respectively. Finally, the Pareto Analysis of Variance (ANOVA) technique is conducted to define the contribution of each of the six design parameters on the L/Dcruise and the |Cm|cruise. More specifically, ic seems to have the most significant effect on L/Dcruise, whereas Λ is the most important parameter for |Cm|cruise.
A Bibliometric Analysis and a Citation Mapping Process for the Role of Soil Recycled Organic Matter and Microbe Interaction due to Climate Change Using Scopus Database
Authors: Vagelas Ioannis , Leontopoulos Stefanos
Published: March 2023

Climate change has drawn the attention not only of scientists but of politicians and societies worldwide. The aim of this paper is to present a method for selecting research studies on climate change, waste management and the role of microbes in the recycling of organic matter in soil that analyze the role of organic agriculture as the main connection between agricultural losses and climate change. VOSviewer version 1.6.18 free software tool was used in this study in order to achieve the bibliometric and mapping approach for studies on the effects of climate change in terms of soil recycled organic matter and microbe interaction. Scopus database (accessed 29 September 2022) indexed a total of 1,245,809 bibliographic items classified into paradigms. The presented documents were downloaded from Scopus as graph-based maps and as distance-based maps in order to reflect the strength of the relation between the items. Climate change includes changes in soil and soil microorganisms as affected by natural climate variations and local weather, which have beneficial or negative effects on soil organic matter. From the examination of the selected papers, it was concluded that climate change and changing precipitation patterns are having an impact on microorganisms, particularly bacterial groups, and thus ecosystem function.
Effects of Different Tillage Practices and Nitrogen Fertiliser Application Rates on Soil-Available Nitrogen
Authors: Zulu Samukelisiwe G. , Motsa Nozipho M. , Magwaza Lembe Samukelo , Ncama Khayelihle , Sithole Nkanyiso J.
Published: March 2023

The dynamics of nitrogen availability in soil result from the net amount of organic and inorganic inputs undergoing decomposition, mineralization, and immobilization. This varies with soil type, climate, and cropping system management practices. Hence, the interaction of these factors in a specific environment is important for nitrogen management and availability in any cropping system. This study evaluated the concentration of nitrate (NO3−) and ammonium (NH4+) in a maize monocropping system in a long-term trial under different tillage practices, i.e., no-till (NT), conventional tillage (CT), and rotational tillage (RT), and with different nitrogen (Urea) fertiliser application rates (0, 100, and 200 kg/ha N). The experimental area was a split plot with randomized tillage strips as the main plots, with the nitrogen fertiliser application rates forming the subplots. The results found the highest concentration of NO3− (7.728 mg/kg) and NH4+ (0.762 mg/kg) under the NT (p < 0.05) compared to the other treatments. CT recorded the lowest amount of NH4+ (0.648 mg/kg) (p < 0.05) compared to RT (0.758 mg/kg), while the concentration of NO3− (6.755 mg/kg) under the CT was relatively higher than under the RT (4.720 mg/kg). The results also showed that the 0–10 cm depth had a significantly (p < 0.05) higher concentration of NO3− (6.787 mg/kg) and NH4+ (0.851 mg/kg), followed at the 20–30 cm depth with 6.229 mg/kg NO3− and 0.832 mg/kg NH4+. Soil samples taken at 10–20 cm depth recorded the lowest NO3− (6.188 mg/kg) and NH4+ (0.485 mg/kg). The study also found the highest NO3− concentration (0.452 mg/kg) with 200 kg/ha N (p < 0.05), while the highest concentration of NH4+ (0.954 mg/kg) was found with 0 kg/ha N. The lowest concentrations of both NO3− (6.333 mg/kg) and NH4+ (0.561 mg/kg) were found with 100 kg/ha N. the no-till also had a significantly (p < 0.05) higher NO3− (8.518 mg/kg) concentration with 200 kg/ha N compared to the CT (6.005 mg/kg) and RT (4.833 mg/kg). On the other hand, more NH4+ (1.478 mg/kg) was found with 0 kg/ha N under the NT compared to the CT (0.717 mg/kg) and the RT (0.669 mg/kg). The concentration of NH4+ was relatively low with 200 kg/ha N in the NT (0.545 mg/kg) and CT (0.598 mg/kg) but higher in the RT (0.813 mg/kg). In conclusion, the NT favoured the availability of NO3− and NH4+ in the studied cropping system; however, the concentration of these was affected by fertiliser application rate and depth. This suggested that each tillage system should be compatible with the type and the rate of inorganic nitrogen fertiliser for better crop production.
The Weather as an Indicator for Decision-Making Support Systems Regarding the Control of Cutworms in Beets and Cereal Leaf Beetles in Cereals and Their Adoption in Farming Practice
Authors: Jakubowska Magdalena , Tratwal Anna , Kachel Magdalena
Published: March 2023

The requirements of integrated control clearly indicate the need to use all available non-chemical methods to protect crop plantations, before deciding on a chemical method. In addition, there is also a provision in the integrated protection guidelines about the need for monitoring and signalling of pests and the recommendation to use available decision support systems (DSS) in chemical protection. The decision-support system facilitates and assists the agricultural producer in fulfilling the above principles and guidelines. The Institute of Plant Protection-National Research Institute (IPP–NRI) has conducted a six-year research program to develop a scientific basis for supporting decisions to control cutworms (Agrotis segetum Schiff et. Den., and Agrotis exclamationis L.) in sugar beets and cereal leaf beetles (Oulema spp.) in cereals. Validation tests in field experiments have demonstrated their effectiveness and their suitability for agricultural practice. Thanks to the obtained results, farmers have access to knowledge supported by solid research and field tests. Based on the obtained results, applications have been developed that will be usable by producers. The applications will assist in deciding the need for chemical protection of sugar beet against cutworms and cereals against cereal leaf beetles, thus recommending treatment only when necessary.
Untargeted Metabolite Profiling of Camellia tetracocca’s Response to an Empoasca onukii Attack Using GC-MS and LC-MS
Authors: Zhang Ni , Tan Weiwen , Luo Guimei , Pu Tianyi , Wang Jinqiu , Zhang Xianhu , Song Yuehua
Published: March 2023

Camellia tetracocca, a kind of tea with local popularity, is unique to southwest China, where it has an important natural heritage and cultural heritage. However, the tea plant and its sprout are frequently attacked on a large-scale by Empoasca onukii. The metabolic mechanisms of the unique plant for defending against these pest insects are unclear. Therefore, we used untargeted gas chromatography–mass spectrometry (GC-MS) and high performance liquid chromatography–mass spectrometry (LC-MS) to compare the metabolite profiles between E. onukii-attacked leaves and healthy leaves. Using GC-MS, 56 metabolites (24 up-regulated, 32 down-regulated) were preliminarily identified. Additionally, 576 metabolites (280 up-regulated, 287 down-regulated) were rudimentarily identified with LC-MS. Differentially abundant metabolites were mainly enriched in the biosynthesis of specialized metabolites. Fourteen accumulated specialized metabolites are related to insect resistance. Mainly, momordicin I and arabidopside B are reportedly involved in the resistance to the insect. Therefore, we conjectured that the accumulation of momordicin I and arabidopside B is involved in the C. tetracocca’s resistance to E. onukii. Our results indicate that these specialized metabolites may be served as candidate biocontrol agents against the pest of E. onukii of C. tetracocca located in the State-owned Pubai Forest Farm.
Air—A New Open Access Journal
Authors: Wong Ling Tim
Published: March 2023

Air (ISSN 2813-4168) is a new peer-reviewed, international, open access online academic journal for scientists in different disciplines related to air’s composition and impacts [...]
Analyzing Health Data Breaches: A Visual Analytics Approach
Authors: Raghupathi Wullianallur , Raghupathi Viju , Saharia Aditya
Published: March 2023

This research studies the occurrence of data breaches in healthcare provider settings regarding patient data. Using visual analytics and data visualization tools, we study the distribution of healthcare breaches by state. We review the main causes and types of breaches, as well as their impact on both providers and patients. The research shows a range of data breach victims. Network servers are the most popular location for common breaches, such as hacking and information technology (IT) incidents, unauthorized access, theft, loss, and improper disposal. We offer proactive recommendations to prepare for a breach. These include, but are not limited to, regulatory compliance, implementing policies and procedures, and monitoring network servers. Unfortunately, the results indicate that the probability of data breaches will continue to rise.
Data-Driven, Physics-Based, or Both: Fatigue Prediction of Structural Adhesive Joints by Artificial Intelligence
Authors: Fernandes Pedro Henrique Evangelista , Silva Giovanni Corsetti , Pitz Diogo Berta , Schnelle Matteo , Koschek Katharina , Nagel Christof , Beber Vinicius Carrillo
Published: March 2023

Here, a comparative investigation of data-driven, physics-based, and hybrid models for the fatigue lifetime prediction of structural adhesive joints in terms of complexity of implementation, sensitivity to data size, and prediction accuracy is presented. Four data-driven models (DDM) are constructed using extremely randomized trees (ERT), eXtreme gradient boosting (XGB), LightGBM (LGBM) and histogram-based gradient boosting (HGB). The physics-based model (PBM) relies on the Findley’s critical plane approach. Two hybrid models (HM) were developed by combining data-driven and physics-based approaches obtained from invariant stresses (HM-I) and Findley’s stress (HM-F). A fatigue dataset of 979 data points of four structural adhesives is employed. To assess the sensitivity to data size, the dataset is split into three train/test ratios, namely 70%/30%, 50%/50%, and 30%/70%. Results revealed that DDMs are more accurate, but more sensitive to dataset size compared to the PBM. Among different regressors, the LGBM presented the best performance in terms of accuracy and generalization power. HMs increased the accuracy of predictions, whilst reducing the sensitivity to data size. The HM-I demonstrated that datasets from different sources can be utilized to improve predictions (especially with small datasets). Finally, the HM-I showed the highest accuracy with an improved sensitivity to data size.
Photoionization and Electron–Ion Recombination in Astrophysical Plasmas
Authors: Hillier D. John
Published: March 2023

Photoionization and its inverse, electron–ion recombination, are key processes that influence many astrophysical plasmas (and gasses), and the diagnostics that we use to analyze the plasmas. In this review we provide a brief overview of the importance of photoionization and recombination in astrophysics. We highlight how the data needed for spectral analyses, and the required accuracy, varies considerably in different astrophysical environments. We then discuss photoionization processes, highlighting resonances in their cross-sections. Next we discuss radiative recombination, and low and high temperature dielectronic recombination. The possible suppression of low temperature dielectronic recombination (LTDR) and high temperature dielectronic recombination (HTDR) due to the radiation field and high densities is discussed. Finally we discuss a few astrophysical examples to highlight photoionization and recombination processes.
The Molecular Mechanisms of Oleanane Aldehyde-β-enone Cytotoxicity against Doxorubicin-Resistant Cancer Cells
Authors: Moiseeva Natalia , Eroshenko Daria , Laletina Lidia , Rybalkina Ekaterina , Susova Olga , Karamysheva Aida , Tolmacheva Irina , Nazarov Mikhail , Grishko Victoria
Published: March 2023

Oleanane aldehyde-β-enone (OA), being the semi-synthetic derivative of the triterpenoid betulin, effectively inhibits the proliferation of HBL-100 and K562 cancer cells (IC50 0.47–0.53 µM), as well as the proliferation of their resistant subclones with high P-gp expression HBL-100/Dox, K562/i-S9 and K562/i-S9_Dox (IC50 0.45−1.24 µM). A molecular docking study, rhodamine efflux test, synergistic test with Dox, and ABC transporter gene expression were used to investigate the ability of OA to act as a P-gp substrate or inhibitor against Dox-resistant cells. We noted a trend toward a decrease in ABCB1, ABCC1 and ABCG2 expression in HBL-100 cells treated with OA. The in silico and in vitro methods suggested that OA is neither a direct inhibitor nor a competitive substrate of P-gp in overexpressing P-gp cancer cells. Thus, OA is able to overcome cellular resistance and can accumulate in Dox-resistant cells to realize toxic effects. The set of experiments suggested that OA toxic action can be attributed to activating intrinsic/extrinsic or only intrinsic apoptosis pathways in Dox-sensitive and Dox-resistant cancer cells, respectively. The cytotoxicity of OA in resistant cells is likely mediated by a mitochondrial cell death pathway, as demonstrated by positive staining with Annexin V–FITC, an increasing number of cells in the subG0/G1 phase, reactive oxygen species generation, mitochondrial dysfunction, cytochrome c migration and caspases-9,-6 activation.
Dysregulation of Serum MicroRNA after Intracerebral Hemorrhage in Aged Mice
Authors: Robles Dominic , Guo De-Huang , Watson Noah , Asante Diana , Sukumari-Ramesh Sangeetha
Published: March 2023

Stroke is one of the most common diseases that leads to brain injury and mortality in patients, and intracerebral hemorrhage (ICH) is the most devastating subtype of stroke. Though the prevalence of ICH increases with aging, the effect of aging on the pathophysiology of ICH remains largely understudied. Moreover, there is no effective treatment for ICH. Recent studies have demonstrated the potential of circulating microRNAs as non-invasive diagnostic and prognostic biomarkers in various pathological conditions. While many studies have identified microRNAs that play roles in the pathophysiology of brain injury, few demonstrated their functions and roles after ICH. Given this significant knowledge gap, the present study aims to identify microRNAs that could serve as potential biomarkers of ICH in the elderly. To this end, sham or ICH was induced in aged C57BL/6 mice (18–24 months), and 24 h post-ICH, serum microRNAs were isolated, and expressions were analyzed. We identified 28 significantly dysregulated microRNAs between ICH and sham groups, suggesting their potential to serve as blood biomarkers of acute ICH. Among those microRNAs, based on the current literature, miR-124-3p, miR-137-5p, miR-138-5p, miR-219a-2-3p, miR-135a-5p, miR-541-5p, and miR-770-3p may serve as the most promising blood biomarker candidates of ICH, warranting further investigation.
HIF-1α-Overexpressing Mesenchymal Stem Cells Attenuate Colitis by Regulating M1-like Macrophages Polarization toward M2-like Macrophages
Authors: Zhu Wenya , Chen Qianqian , Li Yi , Wan Jun , Li Jia , Tang Shuai
Published: March 2023

A modified mesenchymal stem cell (MSC) transplantation is a highly effective and precise treatment for inflammatory bowel disease (IBD), with a significant curative effect. Thus, we aim to examine the efficacy of hypoxia-inducible factor (HIF)–1α-overexpressing MSC (HIF-MSC) transplantation in experimental colitis and investigate the immunity regulation mechanisms of HIF-MSC through macrophages. A chronic experimental colitis mouse model was established using 2,4,6-trinitrobenzene sulfonic acid. HIF-MSC transplantation significantly attenuated colitis in weight loss rate, disease activity index (DAI), colon length, and pathology score and effectively rebuilt the local and systemic immune balance. Macrophage depletion significantly impaired the benefits of HIF-MSCs on mice with colitis. Immunofluorescence analysis revealed that HIF-MSCs significantly decreased the number of M1-like macrophages and increased the number of M2-like macrophages in colon tissues. In vitro, co-culturing with HIF-MSCs significantly decreased the expression of pro-inflammatory factors, C-C chemokine receptor 7 (CCR-7), and inducible nitric oxide synthase (INOS) and increased the expression of anti-inflammatory factors and arginase I (Arg-1) in induced M1-like macrophages. Flow cytometry revealed that co-culturing with HIF-MSCs led to a decrease in the proportions of M1-like macrophages and an increase in that of M2-like macrophages. HIF-MSCs treatment notably upregulated the expression of downstream molecular targets of phosphatidylinositol 3-kinase-γ (PI3K-γ), including HIF-1α and p-AKT/AKT in the colon tissue. A selected PI3K-γ inhibitor, IPI549, attenuated these effects, as well as the effect on M2-like macrophage polarization and inflammatory cytokines in colitis mice. In vitro, HIF-MSCs notably upregulated the expression of C/EBPβ and AKT1/AKT2, and PI3K-γ inhibition blocked this effect. Modified MSCs stably overexpressed HIF-1α, which effectively regulated macrophage polarization through PI3K-γ. HIF-MSC transplantation may be a potentially effective precision therapy for IBD.
Photocatalytic and Glucose Sensing Properties of ZnO-Based Nanocoating
Authors: Bakranova Dina , Seitov Bekbolat , Bakranov Nurlan
Published: March 2023

Here, we report a simple and versatile synthesis of low-dimensional ZnO nanosheet (NS) arrays modified with Fe2O3 (hematite) to assemble photocatalytic coatings and non-enzymatic glucose sensors. Photocatalytic coatings made of widespread elements (zinc and iron) were tested for methylene blue (MB) dye decolorization under ultraviolet and visible (UV-vis) irradiation. A comparative study of unmodified and modified ZnO NS photocatalysts revealed a significant decrease in the dye concentration in 180 min when ZnO/Fe2O3 arrays were used. Size dependence efficiency of the hematite layer deposited onto ZnO is presented. A study of the sensitivity of biosensors made of ZnO nanostructures and ZnO/Fe2O3 nanocomposites for glucose detection showed an improvement in sensitivity with increased Fe2O3 thickness. The structure and morphology of low-dimensional coatings were studied by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray analysis (EDX). The optical properties of nanoarrays showed a red shift of absorption after modifying ZnO with hematite layers, which holds good promise for expanding photocatalytic activity in the visible region.
Assessing the Utility of Hemoglobin, HALP Score, FAR Ratio, and Coagulation Parameters as Predictors for Preterm Birth
Authors: Hrubaru Ingrid , Motoc Andrei , Dumitru Catalin , Bratosin Felix , Fericean Roxana Manuela , Alambaram Satish , Citu Ioana Mihaela , Chicin Gratiana Nicoleta , Erdelean Izabella , Gorun Florin , Citu Cosmin , Popa Zoran Laurentiu
Published: March 2023

Premature birth is a worldwide health issue, posing a high mortality risk for newborns, as well as causing emotional and financial difficulties, and long-term health issues for patients. Identifying effective predictors for preterm birth is essential for prolonging gestation or improving obstetric care. As invasive methods are costly, risky, and not universally available, we aim to assess the predictive capacity of various serum parameters in pregnant women during the third trimester, as a non-invasive alternative. Based on previous studies, it was hypothesized that hemoglobin, the association of hemoglobin, albumin, lymphocyte, and platelets’ (HALP) score, and coagulation parameters such as the prothrombin time (PT), activated partial thromboplastin clotting time (aPTT), D-dimers, and fibrinogen to albumin ratio (FAR) have significant prediction capabilities. With a retrospective design, a total of 161 patients with a history of preterm birth were included in the analysis, being matched 1:1 with a control group of women who gave birth at term. All laboratory samples were collected during the third trimester of pregnancy. The computed area under the curve (AUC) ranged between 0.600 and 0.700 in all six studied parameters, suggesting a fair discrimination. The highest predictive value for preterm birth was observed to be represented by the HALP score with AUC = 0.680 and the highest sensitivity (75%, p-value = 0.001). The highest specificity was achieved by the prothrombin time (69%), and the HALP score was also 69%. The FAR score had an AUC of 0.646, with a sensitivity of 68%, and specificity of 64% (p-value = 0.020). All other variables were significant estimates for the risk of preterm birth, although with lower accuracy. Pregnant women with a hemoglobin level below 12.0 g/dL had a 3.28 higher likelihood of giving birth prematurely. A prothrombin time below 12.5 s determined a 2.11 times higher risk of preterm birth. Similarly, the aPTT below 25 s was linked with 3.24 higher odds of giving birth prematurely. However, the strongest predictors were the D-dimers above 250 ng/mL (OR = 4.26), the FAR score below 0.1, with an odds ratio of 5.30, and the HALP score with a 6.09 OR for a cut-off value above 24. It is important to determine these parameters in pregnant women at risk for giving birth prematurely, but further external validation is required to confirm these findings.
Functional Benefit and Orthotic Effect of Dorsiflexion-FES in Children with Hemiplegic Cerebral Palsy
Authors: Segal Idan , Khamis Sam , Sagie Liora , Genizi Jacob , Azriel David , Katzenelenbogen Sharona , Fattal-Valevski Aviva
Published: March 2023

Functional electrical stimulation of the ankle dorsiflexor (DF-FES) may have advantages over ankle foot orthoses (AFOs) in managing pediatric cerebral palsy (CP). This study assessed the functional benefit and orthotic effect of DF-FES in children with hemiplegic CP. We conducted an open-label prospective study on children with hemiplegic CP ≥ 6 years who used DF-FES for five months. The functional benefit was assessed by repeated motor function tests and the measurement of ankle biomechanical parameters. Kinematic and spatiotemporal parameters were assessed by gait analysis after one and five months. The orthotic effect was defined by dorsiflexion ≥ 0° with DF-FES at either the mid or terminal swing. Among 26 eligible patients, 15 (median age 8.2 years, range 6–15.6) completed the study. After five months of DF-FES use, the results on the Community Balance and Mobility Scale improved, and the distance in the Six-Minute Walk Test decreased (six-point median difference, 95% CI (1.89, 8.1), –30 m, 95% CI (−83.67, −2.6), respectively, p < 0.05) compared to baseline. No significant changes were seen in biomechanical and kinematic parameters. Twelve patients (80%) who showed an orthotic effect at the final gait analysis experienced more supported walking over time, with a trend toward slower walking. We conclude that the continuous use of DF–FES increases postural control and may cause slower but more controlled gait.
The Function of Arbuscular Mycorrhizal Fungi Associated with Drought Stress Resistance in Native Plants of Arid Desert Ecosystems: A Review
Authors: Madouh Tareq A. , Quoreshi Ali M.
Published: March 2023

Drought stress profoundly affects native desert plants’ survival and performance. Among all the abiotic stresses, drought is considered a major constraint that influences the structure and functions of desert ecosystems. Arid desert ecosystems are characterized by prolonged drought, extreme temperatures, high solar radiation, water scarcity, high salinity, scarcity of soil nutrients, and poor soil structure. Such extreme desert environments are the toughest regions on earth, which present enormous challenges in conserving plant survival, growth and reproduction. Despite the predominance of these environmental conditions, native desert plant species that grow in desert environments develop complex adaptation strategies and resistance mechanisms to ameliorate the abiotic and biotic stresses in the extreme environments including changes in biochemical, physiological, and morphological levels. Arbuscular mycorrhizal fungi (AMF) form positive symbiotic associations with a considerable percentage of terrestrial plants as their host, induce distinct impacts on plant growth and protect plants from abiotic stresses. However, it is necessary to advance our understanding of the complex mechanisms associated with AMF-mediated and other dark septate endophytes (DSE)-mediated amelioration of native desert plants’ drought stress resistance and associated biological adjustments such as changes in hormone balance, water and nutrient status, stomatal conductance and osmotic adjustment, antioxidant activity, and photosynthetic activity. This review provides an overview of the relationships of mycorrhiza and fungal endophytes involved in drought stress tolerance, summarizing the current knowledge and presenting possible mechanisms mediated by AMF to stimulate drought tolerance associated with native desert plants. We discuss the research required to fill the gaps and provide suggestions for future research.
Design of Real-Time Monitoring System for Cutting Fluids
Authors: Jurina Frantisek , Peterka Jozef , Vozar Marek , Patoprsty Boris , Vopat Tomas
Published: March 2023

The paper describes the design and implementation of a cutting fluid monitoring system, as well as the design and development of an algorithm to increase the life of the cutting fluid in the machine tool reservoir. Cutting fluids are the most common type of coolant in machining. During its use, it becomes contaminated and gradually degrades until it needs to be replaced with fresh fluid. To increase its effective service life, its parameters should be monitored at regular intervals, and corrective measures such as topping up the fluid quantity and adding inhibitors and additives should be taken if necessary. For this purpose, a conceptual design of a monitoring device was developed, and a prototype device was subsequently manufactured. The device is designed as a floating probe in the storage tank. Therefore, its shape had to be designed to accommodate multiple sensors, batteries, and electronic components while remaining floating and watertight. The designed prototype was made by additive manufacturing and placed in a cutting fluid while being measured at regular intervals. In the event of non-compliant parameters, the algorithm generated corrective actions, and the machine operator could take the required steps to significantly increase the lifetime of the cutting fluid.
Bayesian Spatial Modelling of HIV Prevalence in Jimma Zone, Ethiopia
Authors: Debusho Legesse Kassa , Bedaso Nemso Geda
Published: March 2023

Background: Although the human immunodeficiency virus (HIV) is spatially heterogeneous in Ethiopia, current regional estimates of HIV prevalence hide the epidemic’s heterogeneity. A thorough examination of the prevalence of HIV infection using district-level data could assist to develop HIV prevention strategies. The aims of this study were to examine the spatial clustering of HIV prevalence in Jimma Zone at district level and assess the effects of patient characteristics on the prevalence of HIV infection. Methods: The 8440 files of patients who underwent HIV testing in the 22 Districts of Jimma Zone between September 2018 and August 2019 were the source of data for this study. The global Moran’s index, Getis–Ord Gi* local statistic, and Bayesian hierarchical spatial modelling approach were applied to address the research objectives. Results: Positive spatial autocorrelation was observed in the districts and the local indicators of spatial analysis using the Getis–Ord statistic also identified three districts, namely Agaro, Gomma and Nono Benja, as hotspots, and two districts, namely Mancho and Omo Beyam, as coldspots with 95% and 90% confidence levels, respectively, for HIV prevalence. The results also showed eight patient-related characteristics that were considered in the study were associated with HIV prevalence in the study area. Furthermore, after accounting for these characteristics in the fitted model, there was no spatial clustering of HIV prevalence suggesting the patient characteristics had explained most of the heterogeneity in HIV prevalence in Jimma Zone for the study data. Conclusions: The identification of hotspot districts and the spatial dynamic of HIV infection in Jimma Zone at district level may allow health policymakers in the zone or Oromiya region or at national level to develop geographically specific strategies to prevent HIV transmission. Because clinic register data were used in the study, it is important to use caution when interpreting the results. The results are restricted to Jimma Zone districts and may not be generalizable to Ethiopia or the Oromiya region.
Three Perspectives on the Experience of Support for Family Caregivers in First Nations Communities
Authors: Ward Amber , Buffalo Laurie , McDonald Colleen , L’Heureux Tanya , Charles Lesley , Pollard Cheryl , Tian Peter G , Anderson Sharon , Parmar Jasneet
Published: March 2023

There is a dearth of research on how family caregivers are supported in First Nations. We interviewed family caregivers, health and community providers, and leaders in two Alberta First Nations Communities about their experiences of care and support for the family caregivers in their communities. We employed a qualitative, collaborative participatory action research methodology. We drew on Etuaptmumk, the Mi’kmaw understanding of being in the world is the gift of multiple perspectives. Participants in this research included family caregivers (n = 6), health and community providers (n = 14), and healthcare and community leaders (n = 6). The overarching caregiving theme is the “Hierarchy of challenge”. Six themes capture the challenges faced by family caregivers: (one) “Caregiving is a demanding job”: yet “No one in a sense is taking care of them”; (two) difficult navigation: “I am unable to access that”; (three) delayed assessments and treatment “And I don’t know how they’re being missed”; (four) disconnected health records: “It’s kind of on you to follow up”; (five) racism, “It’s treated differently”; and, (six) social determinants of health, “A lot of these factors have been developing for the longest time”. This study provides evidence that family caregivers’ need to care for and to maintain their own wellbeing is not top of mind in policy or programs in these First Nations communities. As we advocate for support for Canadian family caregivers, we need to ensure that Indigenous family caregivers are also recognized in policy and programs.
Use of Drone Photogrammetry as An Innovative, Competency-Based Architecture Teaching Process
Authors: Rábago Jordi , Portuguez-Castro May
Published: March 2023

The use of drones is becoming increasingly popular in various fields. In the case of education, initiatives have emerged in which they are included as tools to develop student’s knowledge, and their use is becoming more frequent. This research aims to present a case study in which students used drones in an architecture course at a higher education institution in Mexico. It sought to develop transversal competencies in students, such as digital transformation and cutting-edge technologies by studying spaces using photogrammetry with drones. The results showed that students increased their motivation and were able to perform a more detailed analysis of the architectural space in which they conducted the study. Additionally, they were able to capture and analyze information from architectural study processes more quickly. Aerial photogrammetry is a geospatial data collection method that offers several advantages over other methods. These advantages include higher resolution, wide coverage, flexibility, lower costs, and increased safety. Aerial photogrammetry can capture high-resolution images of large areas of land in a single flight, making it an efficient and adaptable tool for a variety of applications and environments. Additionally, it can be more economical and safer than other methods, as it avoids ground contact and reduces risks to personnel and equipment. This study is considered attractive, as it presents an example of the implementation of emerging technologies in architectural education.
Surface Urban Heat Island and Thermal Profiles Using Digital Image Analysis of Cities in the El Bajío Industrial Corridor, Mexico, in 2020
Authors: Medina-Fernández Sandra Lizbeth , Núñez Juan Manuel , Barrera-Alarcón Itzia , Perez-DeLaMora Daniel. A.
Published: March 2023

The Surface Urban Heat Island (SUHI) effect refers to the difference in Land Surface Temperature (LST) between an urban area and its surrounding non-urban area. LST can provide detailed information on the variations in different types of land cover. This study, therefore, analyzes the behavior of LST and SUHIs in fourteen cities in the El Bajío Industrial Corridor, Mexico, using Landsat satellite images from 2020, with QGIS software. It utilizes thermal profiles to identify the land uses that intensify LST, which are essentially those that are anthropologically altered. The results show that the increases in LST and SUHI are more pronounced in cities with greater urban conglomeration, as well as those where there are few green areas and a sizeable industrial or mixed area, with few or no bodies of water. In addition, the increase in temperature in the SUHI is due to certain crops such as vegetables, red fruits, and basic grains such as corn, wheat, and sorghum that use fallow as part of agricultural practices, located around urban areas, which minimizes natural areas with arboreal vegetation.
Homologous High-Level Lipase and Single-Cell Protein Production with Engineered Yarrowia lipolytica via Scale-Up Fermentation for Industrial Applications
Authors: Pan Dujie , Dai Shuhan , Jiao Liangcheng , Zhou Qinghua , Zha Genhan , Yan Jinyong , Han Bingnan , Yan Yunjun , Xu Li
Published: March 2023

Yarrowia lipolytica is a promising feed additives. Here, we aimed to produce extracellular lipases and single-cell proteins (SCPs) at high levels simultaneously through fed-batch fermentation of engineered Y. lipolytica. The parameters for 500 mL shake flask cultures were optimized with a single factorial design. The resultant activity of lipase reached 880.6 U/mL after 84 h of fermentation, and 32.0 g/L fermentation broth of dry SCP was obtained at 120 h. To attain high SCP and lipase productivity, the high-density fed-batch fermentation of Y. lipolytica was scaled up in 10 L, 30 L, and 100 L fermentors. Using glycerol as the sole carbon source, the lipase activity peaked to 8083.3 U/mL, and the final dry SCP weight was 183.1 g/L at 94.6 h in 10 L fermentors. The extracellular lipase activity and SCP weight reached 11,100.0 U/mL and 173.3 g of dry SCP/L at 136 h in 30 L fermentors, respectively. Following 136 h of fed-batch fermentation, the extracellular lipase activity and dry SCP weight reached 8532.0 U/mL and 170.3 g/L in 100 L fermentors, respectively. A balance between the lipase secretion and growth of Y. lipolytica recombinant strain was achieved, indicating that an efficient fermentation strategy could promote further scale-up for industrial SCP production from engineered Y. lipolytica.
Optimizing Task Execution: The Impact of Dynamic Time Quantum and Priorities on Round Robin Scheduling
Authors: Iqbal Mansoor , Ullah Zahid , Khan Izaz Ahmad , Aslam Sheraz , Shaheer Haris , Humayon Mujtaba , Salahuddin Muhammad Asjad , Mehmood Adeel
Published: March 2023

Task scheduling algorithms are crucial for optimizing the utilization of computing resources. This work proposes a unique approach for improving task execution in real-time systems using an enhanced Round Robin scheduling algorithm variant incorporating dynamic time quantum and priority. The proposed algorithm adjusts the time slice allocated to each task based on execution time and priority, resulting in more efficient resource utilization. We also prioritize higher-priority tasks and execute them as soon as they arrive in the ready queue, ensuring the timely completion of critical tasks. We evaluate the performance of our algorithm using a set of real-world tasks and compare it with traditional Round Robin scheduling. The results show that our proposed approach significantly improves task execution time and resource utilization compared to conventional Round Robin scheduling. Our approach offers a promising solution for optimizing task execution in real-time systems. The combination of dynamic time quantum and priorities adds a unique element to the existing literature in this field.
Effect of a Change in the CaCl2/Pectin Mass Ratio on the Particle Size, Rheology and Physical Stability of Lemon Essential Oil/W Emulgels
Authors: Muñoz José , Prieto-Vargas Paula , García Mᵃ Carmen , Alfaro-Rodríguez María-Carmen
Published: March 2023

A three-step (rotor-stator-microfluidization-rotor stator) protocol was used to prepare 15% lemon essential oil in water emulgels using a mixture of Tween 80 and Span 20 surfactants as low molecular mass emulsifiers and 0.4% low-methoxyl citrus peel pectin as a gelling agent. Ca2+ was used as a gel-promoting agent. Different CaCl2/pectin mass ratio values from 0.3 to 0.7 were used. Emulgels showed a microstructure consisting of oil droplets embedded in a sheared gel matrix, as demonstrated by bright field optical microscopy. Laser diffraction tests showed multimodal particle size distributions due to the coexistence of oil droplets and gel-like particles. Multiple light scattering tests revealed that the physical stability of emulgels was longer as the CaCl2/pectin mass ratio decreased and that different destabilization mechanisms took place. Thus, incipient syneresis became more important with increasing CaCl2 concentration, but a parallel creaming mechanism was detected for CaCl2/pectin mass ratio values above 0.5. Dynamic viscoelastic and steady shear flow properties of the emulgels with the lowest and highest CaCl2/pectin mass ratio values were compared as a function of aging time. The lowest ratio yielded an emulgel with enhanced connectivity among fluid units as indicated by its wider linear viscoelastic region, higher storage modulus, loss modulus and viscosity values, and more shear thinning properties than those of the emulgel formulated with the highest CaCl2/pectin mass ratio. The evolution of the dynamic viscoelastic properties with aging time was consistent with the information provided by monitoring scans of backscattering as a function of sample height.