bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Below-Ground Microbial Biostimulant Trichoderma harzianum on Diseases, Insect Community, and Plant Performance in Cucurbita pepo L. under Open Field Conditions
Authors: Forlano Pierluigi , Mang Stefania Mirela , Caccavo Vittoria , Fanti Paolo , Camele Ippolito , Battaglia Donatella , Trotta Vincenzo
Published: November 2022

Agrochemicals are generally used in agriculture to maximize yields and product quality, but their overuse can cause environmental pollution and human health problems. To reduce the off-farm input of chemicals, numerous biostimulant products based on beneficial symbiont plant fungi are receiving a great deal of attention. The evolution of plant diseases and the performance of insects are influenced by plant chemical defences, both of which are, in turn, influenced by below-ground symbionts. Direct and indirect plant defences mediated by belowground symbionts against plant diseases and insect herbivores were demonstrated in greenhouses experiments. However, little attention has been paid to the use of Trichoderma under open field conditions, and no data are available for zucchini (Cucurbita pepo L.) plants in the field. To determine the effects of a commercial Trichoderma harzianum strain T22 on plant viruses, powdery mildew, the arthropod community, and on the agronomic performance associated with zucchini plants, an experiment was conducted in 2022 under open field conditions in South Italy. Our results indicate that T. harzianum T22 makes zucchini plants more attractive to aphids and to Hymenoptera parasitoid but failed to control zucchini pathogens. The complex plant–disease–arthropod–microorganism interactions that occurred in the field during the entire plant cycle are discussed to enrich our current information on the possibilities of using these microorganisms as a green alternative in agriculture.
Immunostimulatory Activity of Lactic Acid Bacteria Cell-Free Supernatants through the Activation of NF-κB and MAPK Signaling Pathways in RAW 264.7 Cells
Authors: Lee Jaekoo , Kim Seonyoung , Kang Chang-Ho
Published: November 2022

Lactic acid bacteria (LAB) can improve host health and has strong potential for use as a health functional food. Specific strains of LAB have been reported to exert immunostimulatory effects. The primary goal of this study was to evaluate the immunostimulatory activities of novel LAB strains isolated from humans and foods and to investigate the probiotic properties of these strains. Cell-free supernatants (CFS) obtained from selected LAB strains significantly increased phagocytosis and level of nitric oxide (NO) and pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 in RAW264.7 macrophage cells. The protein expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2, which are immunomodulators, was also upregulated by CFS treatment. CFS markedly induced the phosphorylation of nuclear factor-κB (NF-κB) and MAPKs (ERK, JNK, and p38). In addition, the safety of the LAB strains used in this study was demonstrated by hemolysis and antibiotic resistance tests. Their stability was confirmed under simulated gastrointestinal conditions. Taken together, these results indicate that the LAB strains selected in this study could be useful as probiotic candidates with immune-stimulating activity.
Screening for Hyperthermophilic Electrotrophs for the Microbial Electrosynthesis of Organic Compounds
Authors: Popall Rabja Maria , Heussner Alenica , Kerzenmacher Sven , Liebgott Pierre-Pol , Pillot Guillaume
Published: November 2022

Microbial electrosynthesis has recently emerged as a promising technology for the sustainable production of organic acids, bioplastics, or biofuels from electricity and CO2. However, the diversity of catalysts and metabolic pathways is limited to mainly mesophilic acetogens or methanogens. Here, eleven hyperthermophilic strains related to Archaeoglobales, Thermococcales, Aquificales, and methanogens were screened for microbial electrosynthesis. The strains were previously isolated from deep-sea hydrothermal vents, where a naturally occurring, spontaneous electrical current can serve as a source of energy for microbial metabolism. After 6 days of incubation in an electrochemical system, all strains showed current consumption, biofilm formation, and small organic molecule production relative to the control. Six selected strains were then incubated over a longer period of time. In the course of one month, a variety of metabolic intermediates of biotechnological relevance such as succinic acid and glycerol accumulated. The production rates and the promotion of specific metabolic pathways seemed to be influenced by the experimental conditions, such as the concentration of CO2 in the gas phase and electron acceptor limitation. Further work is necessary to clearly identify these effects to potentially be able to tune the microbial electrosynthesis of compounds of interest.
Paleowind Directions over the Tarim Block during the Mesoproterozoic, Northwestern China
Authors: Hu Chenlin , Han Changcheng , Ma Jinghui , Deng Li , Zhao Lingfeng
Published: November 2022

The Tarim Block is an ancient plate with a basement of ancient continental crust, which has been separated from the Rodinia supercontinent since the Neoproterozoic. During the Neoproterozoic, which lasted nearly 500 Myr, this block experienced significant evolutionary processes, such as proliferation, radioactive decay of elements, and gradual cooling and solidification. The investigation of Neoproterozoic paleogeography may shed light on the evolution of these geological events. In order to realize this potential, this study aimed to infer paleowind directions over the Tarim Block during each epoch of the Cryogenian–Ediacaran and to constrain the paleogeographic location of the Tarim Block. To this end, outcrop magnetic fabric data were employed to analyze the anisotropy of magnetic susceptibility within the Tarim Block. The anisotropy of magnetic susceptibility measurements yielded mean paleowind directions of 308° ± 69°, 277° ± 78°, and 256° ± 76° from the present north for the Early, Middle, and Late Cryogenian, respectively; the corresponding values for the Early and Late Ediacaran were 237° ± 77° and 254° ± 73° from the present north, respectively. Considering the rotation relationship of the Tarim Block from the Neoproterozoic to the present, the paleowind directions during the Early, Middle, and Late Cryogenian were ~55°, ~35°, and ~35° from the paleo-north, respectively. The paleowind directions during the Early and Late Ediacaran were ~35° and ~60° from paleo-north, respectively. By referring to the correspondence between the paleowind directions over the Tarim Block and trade winds in the Northern Hemisphere, this study provides evidence for the location of the Tarim Block during the Cryogenian–Ediacaran. The main contributions of this study can be summarized as follows: (1) paleowind patterns are established through the analysis of the anisotropy of magnetic susceptibility; (2) the paleogeographic location of the Tarim Block during the Cryogenian–Ediacaran is constrained; and (3) a reference for further study of the paleogeography of the Tarim Block during the Cryogenian–Ediacaran is provided.
Identification and Economic Potentiality of Mineral Sands Resources of Hatiya Island, Bangladesh
Authors: Uddin Md. Ripaj , Khandaker Mayeen Uddin , Akter Nahida , Ahmed Md. Farid , Hossain Syed Md. Minhaz , Gafur Abdul , Abedin Md. Jainal , Rahman Md. Aminur , Idris Abubakr M.
Published: November 2022

Hatiya is the second largest island of Bangladesh and is situated near the Meghna River estuary in the central coastal zone of Bangladesh. This island hosts a few scenic beaches with a huge deposit of mineral sands. Representative mineral sand samples from various beaches of this island were collected during the year 2019, and analyzed for different mineralogical contents using state-of-the-art techniques, such as WD-XRF, XRD, SEM and EDX. This study determined various mineralogical contents, such as SiO2 (73.58%), micas (40.30%), Al2O3 (12.13%), TiO2 (0.56%), MgO (1.31%), Fe2O3 (4.71%), K2O (3.1%), Na2O (1.92%), CaO (3.16%), some earth metals, and heavy minerals, such as ilmenite (14.77%), garnet (11.02%), rutile (14.94%), magnetite (15.26%), and zircon (13.63%), were identified in the analyzed samples. It is suggested that the studied sand can be utilized as a raw material in the glass industry, due to its high SiO2 content. The approximate prices of heavy and light minerals, such as garnet (USD 75–USD 210/mt), ilmenite (USD 110/mt), magnetite (USD 84/mt), rutile (USD 840/mt), zircon (USD 1050/mt) and micas (USD 109/mt), some oxides such as K2O (USD 350–400/mt), CaO (USD 350–450/mt), Al2O3 (USD 1000-USD 1300/mt), TiO2 (USD 4000–4500/mt), and Fe2O3 (USD 650–1500/mt), and some other heavy metals (Rb, Th, Ba, V, Cr, Cs, Ni and Co), indicates a great economic value of the sand of the Hatiya Island beaches. This study recommends that Hatiya Island’s minerals should be mined responsibly and used effectively, to enhance the nation’s economy.
Effects of Pre-Harvest Supplemental UV-A Light on Growth and Quality of Chinese Kale
Authors: Hu Youzhi , Li Xia , He Xinyang , He Rui , Li Yamin , Liu Xiaojuan , Liu Houcheng
Published: November 2022

The effects of supplemental UV-A (385 nm) period and UV-A intensity for 5 days before harvest (DBH) on growth, antioxidants, antioxidant capacity, and glucosinolates contents in Chinese kale (Brassica oleracea var. alboglabra Bailey) were studied in plant factory. In the experiment of the UV-A period, three treatments were designed with 10 W·m−2 UV-A supplement, T1(5 DBH), T2 (10 DBH), and no supplemental UV-A as control. In the experiment of UV-A intensity, four treatments were designed with 5 DBH, control (0 W·m−2), 5 w (5 W·m−2), 10 w (10 W·m−2), and 15 w (15 W·m−2). The growth light is as follows: 250 μmol·m−2·s−1; red light: white light = 2:3; photoperiod: 12/12. The growth and quality of Chinese kale were improved by supplemental UV-A LED. The plant height, stem diameter, and biomass of Chinese kale were the highest in the 5 W·m−2 treatment for 5 DBH. The contents of chlorophyll a, chlorophyll b, and total chlorophyll were only highly increased by 5 W·m−2 UV-A for 5 DBH, while there was no significant difference in the content of carotenoid among all treatments. The contents of soluble sugar and free amino acid were higher only under 10 DBH treatments than in control. The contents of total phenolic and total antioxidant capacity were the highest in 5 W·m−2 treatment for 5 DBH. There was a significant positive correlation between total phenolic content and DPPH and FRAP value. After 5 DBH treatments, the percentages and contents of total aliphatic glucosinolates, sinigrin (SIN), gluconapin (GNA), and glucobrassicanapin (GBN) were highly increased, while the percentages and contents of glucobrassicin (GBS), 4-methoxyglucobrassicin (4-MGBS), and Progoitrin (PRO) were significantly decreased, especially under 10 W·m−2 treatment. Our results show that UV-A LED supplements could improve the growth and quality of Chinese kale, and 5 W·m−2 UV-A LED with 5 DBH might be feasible for Chinese kale growth, and 10 W·m−2 UV-A LED with 5 DBH was better for aliphatic glucosinolates accumulation in Chinese kale.
Capsaicin Decreases Kidney Iron Deposits and Increases Hepcidin Levels in Diabetic Rats with Iron Overload: A Preliminary Study
Authors: López Marisa , Quintero-Macías Laura , Huerta Miguel , Rodríguez-Hernández Alejandrina , Melnikov Valery , Cárdenas Yolitzy , Bricio-Barrios Jaime Alberto , Sánchez-Pastor Enrique , Gamboa-Domínguez Armando , Leal Caridad , Trujillo Xóchitl , Ríos-Silva Mónica
Published: November 2022

Iron overload (IOL) increases the risk of diabetes mellitus (DM). Capsaicin (CAP), an agonist of transient receptor potential vanilloid-1 (TRPV1), reduces the effects of IOL. We evaluated the effects of chronic CAP administration on hepcidin expression, kidney iron deposits, and urinary biomarkers in a male Wistar rat model with IOL and DM (DM-IOL). IOL was induced with oral administration of iron for 12 weeks and DM was induced with streptozotocin. Four groups were studied: Healthy, DM, DM-IOL, and DM-IOL + CAP (1 mg·kg−1·day−1 for 12 weeks). Iron deposits were visualized with Perls tissue staining and a colorimetric assay. Serum hepcidin levels were measured with an enzyme-linked immunosorbent assay. Kidney biomarkers were assayed in 24 h urine samples. In the DM-IOL + CAP group, the total area of iron deposits and the total iron content in kidneys were smaller than those observed in both untreated DM groups. CAP administration significantly increased hepcidin levels in the DM-IOL group. Urinary levels of albumin, cystatin C, and beta-2-microglobulin were similar in all three experimental groups. In conclusion, we showed that in a DM-IOL animal model, CAP reduced renal iron deposits and increased the level of circulating hepcidin.
Visual and Physical Degradation of the Black and White Mosaic of a Roman Domus under Palazzo Valentini in Rome: A Preliminary Study
Authors: Colantonio Claudia , Baldassarri Paola , Avino Pasquale , Astolfi Maria Luisa , Visco Giovanni
Published: November 2022

Palazzo Valentini, the institutional head office of Città Metropolitana di Roma Capitale, stands in in a crucial position in the Roman archaeological and urban contexts, exactly between the Fora valley, Quirinal Hill slopes, and Campus Martius. It stands on a second-century A.D. complex to which belong, between other archeological remains, two richly decorated aristocratic domus. One of these buildings, the domus A, presents an outward porticoed room with a fourth-century AD central impluvium (open air part of the atrium designed to carry away rainwater) with a black/white tiled mosaic pavement, the preservation status of which is compromised by an incoherent degradation product that has caused gradual detachment of the mosaic tiles. To identify the product and determine the causes of degradation, samples of the product were taken and subjected to SEM-EDS, XRF, NMR, FT-IR and GC-MS analyses. The findings reported in this study can help restorers, archaeologists and conservation scientists in order to improve knowledge about the Roman mosaic, its construction phases, conservation problems and proper solutions.
Targeting DNA Topoisomerase II in Antifungal Chemotherapy
Authors: Kondaka Kavya , Gabriel Iwona
Published: November 2022

Topoisomerase inhibitors have been in use clinically for the treatment of several diseases for decades. Although those enzymes are significant molecular targets in antibacterial and anticancer chemotherapy very little is known about the possibilities to target fungal topoisomerase II (topo II). Raising concern for the fungal infections, lack of effective drugs and a phenomenon of multidrug resistance underlie a strong need to expand the range of therapeutic options. In this review paper, we discussed the usefulness of fungal topo II as a molecular target for new drug discovery. On the basis of previously published data, we described structural and biochemical differences between fungal and human enzymes as well as a molecular basis of differential sensitivity to known anticancer drugs targeting the latter. This review focuses especially on highlighting the differences that may underlie the selectivity of action of new inhibitors. Distinct sites within fungal topo II in comparison with human counterparts are observed and should be further studied to understand the significance of those sites and their possible usage in design of new drugs.
Analysis of Phenolic Compounds in Buckwheat (Fagopyrum esculentum Moench) Sprouts Modified with Probiotic Yeast
Authors: Molska Marta , Reguła Julita , Kapusta Ireneusz , Świeca Michał
Published: November 2022

Buckwheat sprouts are a source of various nutrients, e.g., antioxidant flavonoids, which have a positive effect on human health. This study analyzed the content of phenolic compounds and assessed their impact on the antioxidant and anti-inflammatory properties and dietary fiber in modified buckwheat sprouts. For this purpose, the buckwheat seeds were modified by adding Saccharomyces cerevisiae var. boulardii. The modified buckwheat sprouts showed a higher content of total phenol compounds (1526 µg/g d.w.) than the control sprouts (951 µg/g d.w.) and seeds (672 µg/g d.w.). As a consequence, a higher antioxidant activity and anti-inflammatory effect were noted. Probiotic-rich sprouts also had the highest content of total dietary fiber and its soluble fraction. A correlation between phenolic compounds and the antioxidant and anti-inflammatory effects, as well as dietary fiber, was shown. The interaction between dietary fiber and phenolic compounds affects the bioaccessibility, bioavailability, and bioactivity of phenolic compounds in food. The introduction of probiotic yeast into the sprouts had a positive effect on increasing their nutritional value, as well as their antioxidant and anti-inflammatory activity. As a consequence, the nutraceutical potential of the raw material changed, opening a new direction for the use of buckwheat sprouts, e.g., in industry.
Thermal and Rheological Performances Evaluation of a Modified Biopolymer for Fracturing Fluid System
Authors: Ma Guoyan , Wang Le , Hao Chao , Du Chunbao , Ma Hongfei
Published: November 2022

Developing an efficient fracturing fluid system is an enduring hot topic in the petrochemical industries, especially regarding the exploitation of limited oil. Biopolymers, especially polysaccharides (e.g., konjac gum, guar gum), are widely applied as fracturing fluids in fracturing as a result of their advantages. Herein, we propose an easy method of modifying konjac gum (KGM) using isopropanol, sodium hydroxide, and chloroacetic acid to obtain modified konjac glum (MKGM). The MKGM and KGM gels were also obtained by using the self-prepared organic titanium high-temperature stabilizer and organic borate cross-linker. The prepared MKGM was characterized by multiscale techniques, including attenuated total reflection Fourier transform infrared (ATR-FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), and rheology properties. The ATR-FTIR results showed that the etherification modification reaction occurred as designed. The XRD results showed that the regularity of KGM was destroyed after modification. The TGA and DSC results showed that the thermal stability improved. Rheology measurements illustrated that the temperature and shear resistance of MKGM were better than those of KGM. The MKGM gel could be applied in fracturing fluid systems at a lower frequency through viscoelastic measurements.
Platelet Desialylation Is a Novel Mechanism and Therapeutic Target in Daboia siamensis and Agkistrodon halys Envenomation-Induced Thrombocytopenia
Authors: Zhang Cheng , Zhang Zhanfeng , Liang Enyu , Gao Yunlong , Li Hui , Xu Fangfang , Chen Weiye , Liu Ming , Huang Xianzhang
Published: November 2022

Venom-induced thrombocytopenia (VIT) is one of the most important hemotoxic effects of a snakebite, which is often associated with venom-induced consumptive coagulopathy (VICC). Refractory thrombocytopenia without significant coagulation abnormalities has also been reported after envenomation by some viperid snakes; however, the mechanisms are not well understood and therapeutic strategies are lacking. Here, we found that patients injured by Daboia siamensis or Agkistrodon halys snakes, who were resistant to standard antivenom treatment, had developed coagulopathy-independent thrombocytopenia. Venoms from these viperid snakes, rather than from the elapid snake (Bungarus multicinctus), induced platelet surface expression of neuraminidase-1 (NEU-1), and significantly increased the desialylation of the glycoproteins on human platelets. The desialylated platelets caused by viperid snake venoms were further internalized by macrophages, which resulted in reduced platelet numbers in peripheral blood. Importantly, neuraminidase inhibitor significantly decreased viper venom-induced platelet desialylation, therefore inhibiting platelet phagocytosis by macrophages, and alleviating venom-induced thrombocytopenia. Collectively, these findings support an important role for desialylated platelet clearance in the progression of viper envenomation-induced, coagulopathy-independent thrombocytopenia. Our study demonstrates that the neuraminidase inhibitor may be a potential therapy or adjuvant therapy to treat snakebite-induced thrombocytopenia.
Insights into Antimalarial Activity of N-Phenyl-Substituted Cinnamanilides
Authors: Kos Jiri , Degotte Gilles , Pindjakova Dominika , Strharsky Tomas , Jankech Timotej , Gonec Tomas , Francotte Pierre , Frederich Michel , Jampilek Josef
Published: November 2022

Due to the urgent need of innovation in the antimalarial therapeutic arsenal, a series of thirty-seven ring-substituted N-arylcinnamanilides prepared by microwave-assisted synthesis were subjected to primary screening against the chloroquine-sensitive strain of P. falciparum 3D7/MRA-102. The lipophilicity of all compounds was experimentally determined as the logarithm of the capacity factor k, and these data were subsequently used in the discussion of structure-activity relationships. Among the screened compounds, fourteen derivatives exhibited IC50 from 0.58 to 31 µM, whereas (2E)-N-(4-bromo-2-chlorophenyl)-3-phenylprop-2-enamide (24) was the most effective agent (IC50 = 0.58 µM). In addition, (2E)-N-[2,6-dibromo-4-(trifluoromethyl)- phenyl]-3-phenylprop-2-enamide (36), (2E)-N-[4-nitro-3-(trifluoromethyl)phenyl]-3-phenylprop- 2-enamide (18), (2E)-N-(2-bromo-5-fluorophenyl)-3-phenylprop-2-enamide (23), and (2E)-3-phenyl-N-(3,4,5-trichlorophenyl)prop-2-enamide (33) demonstrated efficacy in the IC50 range from 2.0 to 4.3 µM, comparable to the clinically used standard chloroquine. The results of a cell viability screening performed using THP1-Blue™ NF-κB cells showed that none of these highly active compounds displayed any significant cytotoxic effect up to 20 μM, which makes them promising Plasmodium selective substances for further investigations.
First Synthesis of 3-Glycopyranosyl-1,2,4-Triazines and Some Cycloadditions Thereof
Authors: Bokor Éva , Ferenczi Attila , Hashimov Mahir , Juhász-Tóth Éva , Götz Zsófia , Zaki Alshimaa Ibrahim , Somsák László
Published: November 2022

C-glycopyranosyl derivatives of six-membered heterocycles are scarcely represented in the chemical literature and the title 3-glycopyranosyl-1,2,4-triazines are completely unknown. In this paper, the first synthesis of this compound class is accomplished by the cyclocondensation of C-glycosyl formamidrazones and 1,2-dicarbonyl derivatives. In addition, the synthesis of C-glycopyranosyl 1,2,4-triazin-5(4H)-ones was also carried out by the transformation of the above formamidrazones with α-keto-carboxylic esters. Inverse electron demand Diels–Alder reactions of 3-glycopyranosyl-1,2,4-triazines with a bicyclononyne derivative yielded the corresponding annulated 2-glycopyranosyl pyridines.
Toughening Polyamidoamine Hydrogels through Covalent Grafting of Short Silk Fibers
Authors: Maggi Filippo , Manfredi Amedea , Carosio Federico , Maddalena Lorenza , Alongi Jenny , Ferruti Paolo , Ranucci Elisabetta
Published: November 2022

Linear amphoteric polyamidoamines (PAAs) are usually water-soluble, biodegradable and biocompatible. Crosslinked PAAs form in water hydrogels, retaining most of the favorable properties of their linear counterparts. The hydrogels prepared by the radical post-polymerization of the oligo-α,ω-bisacrylamido-terminated PAA called AGMA1, obtained by the polyaddition of 4-aminobutylguanidine (agmatine) with 2,2-bis(acrylamido)acetic acid, exhibit excellent cell-adhesion properties both in vitro and in vivo. However, due to their low mechanical strength, AGMA1 hydrogels cannot be sewn to biological tissues and need to be reinforced with fibrous materials. In this work, short silk fibers gave excellent results in this sense, proving capable of establishing covalent bonds with the PAA matrix, thanks to their lysine content, which provided amino groups capable of reacting with the terminal acrylamide groups of the AGMA1 precursor in the final crosslinking phase. Morphological analyses demonstrated that the AGMA1 matrix was intimately interconnected and adherent to the silk fibers, with neither visible holes nor empty volumes. The silk/H-AGMA1 composites were still reversibly swellable in water. In the swollen state, they could be sewn and showed no detachment between fibers and matrix and exhibited significantly improved mechanical properties compared with the plain hydrogels, particularly as regards their Young’s modulus and elongation at break.
Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding
Authors: Lu Chang , Lopez Anand , Zheng Jinkai , Liu Juewen
Published: November 2022

The reliable, readily accessible and label-free measurement of aptamer binding remains a challenge in the field. Recent reports have shown large changes in the intrinsic fluorescence of DNA upon the formation of G-quadruplex and i-motif structures. In this work, we examined whether DNA intrinsic fluorescence can be used for studying aptamer binding. First, DNA hybridization resulted in a drop in the fluorescence, which was observed for A30/T30 and a 24-mer random DNA sequence. Next, a series of DNA aptamers were studied. Cortisol and Hg2+ induced fluorescence increases for their respective aptamers. For the cortisol aptamer, the length of the terminal stem needs to be short to produce a fluorescence change. However, caffeine and adenosine failed to produce a fluorescence change, regardless of the stem length. Overall, using the intrinsic fluorescence of DNA may be a reliable and accessible method to study a limited number of aptamers that can produce fluorescence changes.
Off-Line SPE LC-LRMS Polyphenolic Fingerprinting and Chemometrics to Classify and Authenticate Spanish Honey
Authors: García-Seval Víctor , Saurina Javier , Sentellas Sònia , Núñez Oscar
Published: November 2022

The feasibility of non-targeted off-line SPE LC-LRMS polyphenolic fingerprints to address the classification and authentication of Spanish honey samples based on both botanical origin (blossom and honeydew honeys) and geographical production region was evaluated. With this aim, 136 honey samples belonging to different botanical varieties (multifloral and monofloral) obtained from different Spanish geographical regions with specific climatic conditions were analyzed. Polyphenolic compounds were extracted by off-line solid-phase extraction (SPE) using HLB (3 mL, 60 mg) cartridges. The obtained extracts were then analyzed by C18 reversed-phase LC coupled to low-resolution mass spectrometry in a hybrid quadrupole-linear ion trap mass analyzer and using electrospray in negative ionization mode. Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were employed to assess the pattern recognition capabilities of the obtained fingerprints to address honey classification and authentication. In general, a good sample discrimination was accomplished by PLS-DA, being able to differentiate both blossom-honey and honeydew-honey samples according to botanical varieties. Multiclass predictions by cross-validation for the set of blossom-honey samples showed sensitivity, specificity, and classification ratios higher than 60%, 85%, and 87%, respectively. Better results were obtained for the set of honeydew-honey samples, exhibiting 100% sensitivity, specificity, and classification ratio values. The proposed fingerprints also demonstrated that they were good honey chemical descriptors to deal with climatic and geographical issues. Characteristic polyphenols of each botanical variety were tentatively identified by LC-MS/MS in multiple-reaction monitoring mode to propose possible honey markers for future experiments (i.e., naringin for orange/lemon blossom honeys, syringic acid in thyme honeys, or galangin in rosemary honeys).
The Effects of the Addition of Polyurethane–MgO Nanohybrids on the Mechanical Properties of Ordinary Portland Cement Paste
Authors: Fang Yu , Ning Weiqing , Li Yuan , Li Fang , Pournajaf Reza , Hamawandi Bejan
Published: November 2022

One of the most important methods of controlling the properties of concrete and cement-based materials is to control the rate and kinetics of cement hydration. In the present study, novel flexible polyurethane-decorated MgO nanohybrids were synthesized using a simple chemical method, added to cement paste in different amounts, and utilized as an effective mechanical performance-enhancing factor for cement paste. It was observed that by adding 3 wt% synthesized PU-MgO nanohybrids to cement paste, its mechanical properties were improved and its compressive strength and flexural strength were increased by up to 13% and 15%, respectively, compared to the plain cement, after 45 days. The effect mechanism of adding PU–MgO nanoparticles on the properties of the cement paste was investigated. The addition of PU–MgO nanohybrids increased the pozzolanic reactions and formed more C-S-H phases.
Polypyrrole-Assisted Ag Doping Strategy to Boost Co(OH)2 Nanosheets on Ni Foam as a Novel Electrode for High-Performance Hybrid Supercapacitors
Authors: Arbi Hammad , Yadav Anuja , Anil Kumar Yedluri , Moniruzzaman Md , Alzahmi Salem , Obaidat Ihab
Published: November 2022

Battery-type electrode materials have attracted much attention as efficient and unique types of materials for hybrid battery supercapacitors due to their multiple redox states and excellent electrical conductivity. Designing composites with high chemical and electrochemical stabilities is beneficial for improving the energy storage capability of battery-type electrode materials. We report on an interfacial engineering strategy to improve the energy storage performance of a Co(OH)2-based battery-type material by constructing polypyrrole-assisted and Ag-doped (Ag-doped@Co(OH)2@polypyrrole) nanosheets (NSs) on a Ni foam using a hydrothermal process that provides richer electroactive sites, efficient charge transportation, and an excellent mechanical stability. Physical characterization results revealed that the subsequent decoration of Ag nanoparticles on Co(OH)2 nanoparticles offered an efficient electrical conductivity as well as a reduced interface adsorption energy of OH- in Co(OH)2 nanoparticles as compared to Co(OH)2@polypyrrole-assisted nanoparticles without Ag particles. The heterogeneous interface of the Ag-doped@Co(OH)2@polypyrrole composite exhibited a high specific capacity of 291.2 mAh g−1 at a current density of 2 A g−1, and showed a good cycling stability after 5000 cycles at 5 A g−1. The specific capacity of the doped electrode was enhanced approximately two-fold compared to that of the pure electrode. Thus, the fabricated Ag-doped@Co(OH)2@polypyrrole nanostructured electrodes can be a potential candidate for fabricating low-cost and high-performance energy storage supercapacitor devices.
Synergistic Effects of Graphene Oxide and Pesticides on Fall Armyworm, Spodoptera frugiperda
Authors: Li Xue , Wang Qinying , Wang Xiuping , Wang Zhenying
Published: November 2022

Fall armyworm Spodoptera frugiperda, a native insect pest in tropical and subtropical America, has rapidly spread to most parts of China and become a major pest of corn and other crops since invading in early January 2019. As an emergency and important control measure, chemical control of S. frugiperda has the advantages of quick effect and low cost. However, long-term and large-scale use of pesticides might pollute the environment and increase pest resistance. By improving the control effect and reducing the dosage of chemical pesticides, graphene oxide (GO) is used synergistically with insecticides to increase control efficacy to achieve low-cost and sustainable management of insect pests as a new type of synergist. In this study, graphene oxide was compounded with insecticides to form nanocomposites. To clarify pest physiological responses, the laboratory toxicity of graphene oxide-insecticide nanocomposites was measured on the larvae of S. frugiperda. The results demonstrated that GO could enhance the activity of four selected pesticides: chlorantraniliprole (Chl), beta cypermethrin (Bet), methoxyhydrazide (Met) and spinetoram (Spi). Compared with pesticides alone, the toxicity of Chl-GO, Bet-GO, Met-GO and Spi-GO mixtures to the third instar larvae of S. frugiperda increased by 1.56, 1.54, 2.53 and 1.74 times, respectively. The easy preparation and higher bioactivity of GO-pesticide nanocomposites indicated their promising application potential in pest control.
Theoretical Optical Output Power Improvement of InGaN-Based Violet Laser Diode Using AlGaN/GaN Composite Last Quantum Barrier
Authors: Zhang Zhenzhuo , Yang Jing , Zhao Degang , Liang Feng , Chen Ping , Liu Zongshun
Published: November 2022

Electron leakage has an adverse influence on the optical output power for laser diodes (LDs), especially where the conventional last quantum barrier (LQB) in the multiple quantum well (MQW) active region may cause severe leakage problems. In this article, a composite last quantum barrier (CLQB) composed of p-type doped AlGaN (p-AlGaN) and unintentionally doped GaN (u-GaN) layers is designed to replace the conventional one, for overcoming the problem of electron overflow. Theoretical calculations with LASTIP software demonstrate that CLQB with optimized parameters of Al composition, thickness and p-type doping concentration of the p-AlGaN layer in the CLQB can have a 50% improvement in slope efficiency (SE) compared with the conventional structure LD. This will help to realize a higher optical output power in InGaN-based violet LDs.
Investigations on Grating-Enhanced Waveguides for Wide-Angle Light Couplings
Authors: Gu Yitong , Wang Ning , Shang Haorui , Yu Fei , Hu Lili
Published: November 2022

As a universal physical scheme, effective light couplings to waveguides favor numerous applications. However, the low coupling efficiency at wide angles prohibits this fundamental functionality and thus lowers the performance levels of photonic systems. As previously found, the transmission gratings patterned on waveguide facets could significantly improve the large-angle-inputted efficiency to the order of 10−1. Here, we continue this study with a focus on a common scenario, i.e., a grating-modified waveguide excited by the Gaussian beam. A simplified 2D theoretical model is firstly introduced, proving that the efficiency lineshape could be well flattened by elaborately arranged diffractive gratings. For demonstration, subsequent explorations for proper grating geometries were conducted, and four structural configurations were selected for later full-wave numerical simulations. The last comparison studies showcase that the analytical method approximates the finite element method-based modelings. Both methods highlight grating-empowered coupling efficiencies, being 2.5 bigger than the counterparts of the previously reported seven-ring structure. All in all, our research provides instructions to simulate grating effects on the waveguide’s light-gathering abilities. Together with algorithm-designed coupling structures, it would be of great interest to further benefit real applications, such as bioanalytical instrumentation and quantum photon probes.
Remineralizing Potential of Natural Nano-Hydroxyapatite Obtained from Epinephelus chlorostigma in Artificially Induced Early Enamel Lesion: An In Vitro Study
Authors: Mathirat Ashwathi , Dalavi Pandurang Appana , Prabhu Ashwini , G.V. Yashaswini Devi , Anil Sukumaran , Senthilkumar Kalimuthu , Seong Gi Hun , Sargod Sharan S. , Bhat Sham S. , Venkatesan Jayachandran
Published: November 2022

Dental caries is a common problem in adolescents, leading to permanent loss of teeth or cavitation. Caries is a continuous process wherein demineralization and remineralization occur regularly. Hydroxyapatite (HA) is one of the most biocompatible and bioactive materials, as it closely resembles the mineral composition of teeth. The present study deals with isolating hydroxyapatite from fish bone (Epinephelus chlorostigma) by alkaline hydrolysis and thermal calcination. The isolated nano HA was characterized using FT-IR, XRD, TGA, FE-SEM-EDX, and HR-TEM analysis. The nano HA isolated by alkaline hydrolysis is nontoxic, and the cells are viable. The isolated HA enhances the proliferation of L929 cells. The remineralization potential of the extracted nano HA was evaluated in healthy premolars by DIAGNOdent/laser fluorescence quantification, surface microhardness test, and SEM-EDX analysis. Surface morphological observations in SEM and EDX analyses show that thermally calcined HA and alkali-treated HA can induce mineralization and deposit minerals. Therefore, HA obtained from Epinephelus chlorostigma could be a potential biomaterial for treating early caries.
Comparative Review on the Aqueous Zinc-Ion Batteries (AZIBs) and Flexible Zinc-Ion Batteries (FZIBs)
Authors: Al-Amin Md. , Islam Saiful , Shibly Sayed Ul Alam , Iffat Samia
Published: November 2022

Lithium-ion batteries (LIBs) have been considered an easily accessible battery technology because of their low weight, cheapness, etc. Unfortunately, they have significant drawbacks, such as flammability and scarcity of lithium. Since the components of zinc-ion batteries are nonflammable, nontoxic, and cheap, AZIBs could be a suitable replacement for LIBs. In this article, the advantages and drawbacks of AZIBs over other energy storage devices are briefly discussed. This review focused on the cathode materials and electrolytes for AZIBs. In addition, we discussed the approaches to improve the electrochemical performance of zinc batteries. Here, we also discussed the polymer gel electrolytes and the electrodes for flexible zinc-ion batteries (FZIBs). Moreover, we have outlined the importance of temperature and additives in a flexible zinc-ion battery. Finally, we have discussed anode materials for both AZIBs and FZIBs. This review has summarized the advantages and disadvantages of AZIBs and FZIBs for future applications in commercial battery technology.
Tailoring Nonlinear Metamaterials for the Controlling of Spatial Quantum Entanglement
Authors: Ming Yang , Liu Yuan , Chen Wei , Yan Yusen , Zhang Huiguo
Published: November 2022

The high designability of metamaterials has made them an attractive platform for devising novel optoelectronic devices. The demonstration of nonlinear metamaterials further indicates their potential in developing quantum applications. Here, we investigate designing nonlinear metamaterials consisting of the 3-fold (C3) rotationally symmetrical nanoantennas for generating and modulating entangled photons in the spatial degrees of freedom. Through tailoring the geometry and orientation of the nanoantennas, the parametric down conversion process inside the metamaterials can be locally engineered to generate entangled states with desired spatial properties. As the orbital angular momentum (OAM) states are valuable for enhancing the data capacity of quantum information systems, the photonic OAM entanglement is practically considered. With suitable nanostructure design, the generation of OAM entangled states is shown to be effectively realized in the discussed nonlinear metamaterial system. The nonlinear metamaterials present a perspective to provide a flexible platform for quantum photonic applications.
COVID-19 in Low and Middle Income Countries
Authors: Colebunders Robert , Siewe Fodjo Joseph Nelson
Published: November 2022

The COVID-19 pandemic is by far the worst epidemic in the last century, causing more than 6 [...]
Glycyrrhizin Interacts with TLR4 and TLR9 to Resolve P. aeruginosa Keratitis
Authors: Somayajulu Mallika , McClellan Sharon A. , Farooq Shukkur Muhammed , Pitchaikannu Ahalya , Xu Shunbin , Hazlett Linda
Published: November 2022

This study tests the mechanism(s) of glycyrrhizin (GLY) protection against P. aeruginosa keratitis. Female C57BL/6 (B6), TLR4 knockout (TLR4KO), myeloid specific TLR4KO (mTLR4KO), their wildtype (WT) littermates, and TLR9 knockout (TLR9KO) mice were infected with P. aeruginosa KEI 1025 and treated with GLY or PBS onto the cornea after infection. Clinical scores, photography with a slit lamp, RT-PCR and ELISA were used. GLY effects on macrophages (Mϕ) and polymorphonuclear neutrophils (PMN) isolated from WT and mTLR4KO and challenged with KEI 1025 were also tested. Comparing B6 and TLR4KO, GLY treatment reduced clinical scores and improved disease outcome after infection and decreased mRNA expression levels in cornea for TLR4, HMGB1, and RAGE in B6 mice. TLR9 mRNA expression was significantly reduced by GLY in both mouse strains after infection. GLY also significantly reduced HMGB1 (B6 only) and TLR9 protein (both B6 and TLR4KO). In TLR9KO mice, GLY did not significantly reduce clinical scores and only slightly improved disease outcome after infection. In these mice, GLY significantly reduced TLR4, but not HMGB1 or RAGE mRNA expression levels after infection. In contrast, in the mTLR4KO and their WT littermates, GLY significantly reduced corneal disease, TLR4, TLR9, HMGB1, and RAGE corneal mRNA expression after infection. GLY also significantly reduced TLR9 and HMGB1 corneal protein levels in both WT and mTLR4KO mice. In vitro, GLY significantly lowered mRNA expression levels for TLR9 in both Mϕ and PMN isolated from mTLR4KO or WT mice after incubation with KEI 1025. In conclusion, we provide evidence to show that GLY mediates its effects by blocking TLR4 and TLR9 signaling pathways and both are required to protect against disease.
Quantitative G6PD Deficiency Screening in Routine Malaria Diagnostic Units in the Brazilian Amazon (SAFEPRIM): An Operational Mixed-Methods Study
Authors: Brito-Sousa Jose Diego , Murta Felipe , Vitor-Silva Sheila , Sampaio Vanderson , Mendes Maxwell , Souza Brenda , Batista Talita , Santos Alicia , Marques Leonardo , Barbosa Laila , Balieiro Patricia , Silva-Neto Alexandre , Rabello Renata , Brito Marcelo , Silva Emanuelle , Rodovalho Sheila , Arcanjo Ana Ruth , Melo Gisely , Recht Judith , Domingo Gonzalo J. , Valle Suiane , Souza Rodrigo , Nakagawa Theresa , Monteiro Wuelton , Lacerda Marcus
Published: November 2022

Background: Glucose-6-phosphate dehydrogenase (G6PD) deficiency testing is not routinely performed before primaquine treatment in most Plasmodium vivax endemic areas, despite the risk of primaquine-associated hemolysis. This is due to the operational challenges associated with pragmatic G6PD testing and as such needs to be addressed. Methods and findings: This mixed-methods operational study was aimed at implementing the quantitative point-of-care StandardTM G6PD (SD Biosensor, Korea) screening test in malaria treatment units (MTUs) in the municipalities of Rio Preto da Eva and Mâncio Lima, in the Brazilian Amazon, between mid-January 2020 and December 2020. In total, 1286 P. vivax cases were treated based on the Standard G6PD test: 1230 had activity equal to or greater than 4.0 U/g Hb, and 56 less than 4.0 U/g Hb. No G6PD deficient (G6PDd) genotypes were found in 96 samples from the 1230, and only 21 of the 56 G6PDd cases had confirmed G6PDd genotypes. Evaluations were conducted on the proficiency of health care professionals (HCPs) training to perform the test, the reliability of testing performed in the field, and the perceptions of HCPs and patients about the implementation. Post-training proficiency was 73.4% after a 4-hour training session. This study revealed that locations with lower malaria caseloads will need regular refresher training. The test was well accepted by both HCPs and patients. Signs and symptoms of hemolysis were not always associated with malaria treatment drugs by HCPs and patients. Interpretation: Point-of-care quantitative G6PD testing can be performed at MTUs in the Brazilian Amazon to inform treatment decisions with primaquine. Limitations related to technical and cultural aspects need to be addressed further when expanding screening to larger areas.
Mycobacterium bovis Strain Ravenel Is Attenuated in Cattle
Authors: Hadi Syeda A. , Brenner Evan P. , Palmer Mitchell V. , Waters W. Ray , Thacker Tyler C. , Vilchèze Catherine , Larsen Michelle H. , Jacobs William R. , Sreevatsan Srinand
Published: November 2022

Mycobacterium tuberculosis variant bovis (MBO) has one of the widest known mammalian host ranges, including humans. Despite the characterization of this pathogen in the 1800s and whole genome sequencing of a UK strain (AF2122) nearly two decades ago, the basis of its host specificity and pathogenicity remains poorly understood. Recent experimental calf infection studies show that MBO strain Ravenel (MBO Ravenel) is attenuated in the cattle host compared to other pathogenic strains of MBO. In the present study, experimental infections were performed to define attenuation. Whole genome sequencing was completed to identify regions of differences (RD) and single nucleotide polymorphisms (SNPs) to explain the observed attenuation. Comparative genomic analysis of MBO Ravenel against three pathogenic strains of MBO (strains AF2122-97, 10-7428, and 95-1315) was performed. Experimental infection studies on five calves each, with either MBO Ravenel or 95-1315, revealed no visible lesions in all five animals in the Ravenel group despite robust IFN-γ responses. Out of 486 polymorphisms in the present analysis, 173 were unique to MBO Ravenel among the strains compared. A high-confidence subset of nine unique SNPs were missense mutations in genes with annotated functions impacting two major MBO survival and virulence pathways: (1) Cell wall synthesis & transport [espH (A103T), mmpL8 (V888I), aftB (H484Y), eccC5 (T507M), rpfB (E263G)], and (2) Lipid metabolism & respiration [mycP1(T125I), pks5 (G455S), fadD29 (N231S), fadE29 (V360G)]. These substitutions likely contribute to the observed attenuation. Results from experimental calf infections and the functional attributions of polymorphic loci on the genome of MBO Ravenel provide new insights into the strain’s genotype-disease phenotype associations.
Is Hepatitis E Virus a Neglected or Emerging Pathogen in Egypt?
Authors: Sayed Ibrahim M. , Abdelwahab Sayed F.
Published: November 2022

Though Egypt ranks among the top countries for viral hepatitis and death-related liver disease, Hepatitis E virus (HEV) is a neglected pathogen. Living in villages and rural communities with low sanitation, use of underground well water and contact with animals are the main risk factors for HEV infection. Domestic animals, especially ruminants and their edible products, are one source of infection. Contamination of water by either human or animal stools is the main route of infection. In addition, HEV either alone or in coinfection with other hepatotropic viruses has been recorded in Egyptian blood donors. HEV seropositivity among Egyptian villagers was 60–80%, especially in the first decade of life. Though HEV seropositivity is the highest among Egyptians, HEV infection is not routinely diagnosed in Egyptian hospitals. The initial manifestations of HEV among Egyptians is a subclinical infection, although progression to fulminant hepatic failure has been recorded. With the improvement in serological and molecular approaches and increasing research on HEV, it is becoming clear that HEV represents a threat for Egyptians and preventive measures should be considered to reduce the infection rate and possible complications