bluuebunny/crossref_metadata_2025 huggingface dataset:
Attitudes Toward and Use of Prostate-Specific Antigen Testing Among Urologists and General Practitioners in Germany: A Survey
Authors: Kappen Sanny , Jürgens Verena , Freitag Michael H. , Winter Alexander
Published: June 2021

Background

In 2020, around 1.4 million new prostate cancer (PCa) cases were recorded worldwide. Early detection of PCa by prostate-specific antigen (PSA) screening remains debated, leading to different specialist-specific recommendations in PCa guidelines. This study aimed to assess attitudes toward and use of PSA testing among urologists in Germany and general practitioners (GPs) in Lower Saxony (Germany).

Methods

A nationwide questionnaire was sent to urologists via the mailing lists of the Professional Association of German Urologists and the German Urological Society. A version of the questionnaire for GPs was sent to email addresses via the Association of Statutory Health Insurance Physicians Lower Saxony. The online questionnaires covered use of PSA testing, information communication, handling of test results, and handling of/knowledge about national and international guidelines and recommendations on early detection of PCa. Statistical analysis was performed at a descriptive level.

Results

In total, 432 of 6,568 urologists (6.6%) and 96 of 1,579 GPs (6.1%) participated in this survey. Urologists and GPs differed in their attitudes and approaches toward PSA testing. Most urologists (86.8%, n=375) judged the test as “very meaningful” or “meaningful”, compared with 52.1% (n=50) of GPs. Almost two-thirds of the urologists (64.4%, n=278) viewed the PCa mortality reduction by PSA testing as proven, compared with one-fifth of GPs (20.8%, n=20). Almost 80% of male urologists (79.9%, n=291) indicated that they would undergo a PSA test in the future (again), compared with 55.1% of male GPs (n=38). In addition, 56.3% (n=243) of urologists stated that “considerably more than half” or “almost all” men aged 45 years or older received a PSA test, compared with 19.8% (n=19) of GPs.

Conclusions

Urologists are more convinced about the PSA test than GPs. PSA testing is therefore used more often in urological settings, although the preselected patient population must be considered. In accordance with specialist-specific recommendations, GPs show a more reserved approach toward PSA testing. Instead of focusing on different attitudes and recommendations on PSA testing, the exchange between specialist groups should be improved to achieve a consistent approach to PSA testing.
The Nonsteroidal Anti-Inflammatory Drug Ketorolac Alters the Small Intestinal Microbiota and Bile Acids Without Inducing Intestinal Damage or Delaying Peristalsis in the Rat
Authors: Hutka Barbara , Lázár Bernadette , Tóth András S. , Ágg Bence , László Szilvia B. , Makra Nóra , Ligeti Balázs , Scheich Bálint , Király Kornél , Al-Khrasani Mahmoud , Szabó Dóra , Ferdinandy Péter , Gyires Klára , Zádori Zoltán S.
Published: June 2021

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) induce significant damage to the small intestine, which is accompanied by changes in intestinal bacteria (dysbiosis) and bile acids. However, it is still a question of debate whether besides mucosal inflammation also other factors, such as direct antibacterial effects or delayed peristalsis, contribute to NSAID-induced dysbiosis. Here we aimed to assess whether ketorolac, an NSAID lacking direct effects on gut bacteria, has any significant impact on intestinal microbiota and bile acids in the absence of mucosal inflammation. We also addressed the possibility that ketorolac-induced bacterial and bile acid alterations are due to a delay in gastrointestinal (GI) transit.

Methods: Vehicle or ketorolac (1, 3 and 10 mg/kg) were given to rats by oral gavage once daily for four weeks, and the severity of mucosal inflammation was evaluated macroscopically, histologically, and by measuring the levels of inflammatory proteins and claudin-1 in the distal jejunal tissue. The luminal amount of bile acids was measured by liquid chromatography-tandem mass spectrometry, whereas the composition of microbiota by sequencing of bacterial 16S rRNA. GI transit was assessed by the charcoal meal method.

Results: Ketorolac up to 3 mg/kg did not cause any signs of mucosal damage to the small intestine. However, 3 mg/kg of ketorolac induced dysbiosis, which was characterized by a loss of families belonging to Firmicutes (Paenibacillaceae, Clostridiales Family XIII, Christensenellaceae) and bloom of Enterobacteriaceae. Ketorolac also changed the composition of small intestinal bile by decreasing the concentration of conjugated bile acids and by increasing the amount of hyodeoxycholic acid (HDCA). The level of conjugated bile acids correlated negatively with the abundance of Erysipelotrichaceae, Ruminococcaceae, Clostridiaceae 1, Muribaculaceae, Bacteroidaceae, Burkholderiaceae and Bifidobacteriaceae. Ketorolac, under the present experimental conditions, did not change the GI transit.

Conclusion: This is the first demonstration that low-dose ketorolac disturbed the delicate balance between small intestinal bacteria and bile acids, despite having no significant effect on intestinal mucosal integrity and peristalsis. Other, yet unidentified, factors may contribute to ketorolac-induced dysbiosis and bile dysmetabolism.
PK11195 Protects From Cell Death Only When Applied During Reperfusion: Succinate-Mediated Mechanism of Action
Authors: Seidlmayer Lea K. , Hanson Benjamin J. , Thai Phung N. , Schaefer Saul , Bers Donald M. , Dedkova Elena N.
Published: June 2021

Aim: Reperfusion after myocardial ischemia causes cellular injury, in part due to changes in mitochondrial Ca2+ handling, oxidative stress, and myocyte energetics. We have previously shown that the 18-kDa translocator protein of the outer mitochondrial membrane (TSPO) can modulate Ca2+ handling. Here, we aim to evaluate the role of the TSPO in ischemia/reperfusion (I/R) injury.

Methods: Rabbit ventricular myocytes underwent simulated acute ischemia (20 min) and reperfusion (at 15 min, 1 h, and 3 h) in the absence and presence of 50 μM PK11195, a TSPO inhibitor. Cell death was measured by lactate dehydrogenase (LDH) assay, while changes in mitochondrial Ca2+, membrane potential (ΔΨm), and reactive oxygen species (ROS) generation were monitored using confocal microscopy in combination with fluorescent indicators. Substrate utilization was measured with Biolog mitochondrial plates.

Results: Cell death was increased by ~200% following I/R compared to control untreated ventricular myocytes. Incubation with 50 μM PK11195 during both ischemia and reperfusion did not reduce cell death but increased mitochondrial Ca2+ uptake and ROS generation. However, application of 50 μM PK11195 only at the onset and during reperfusion effectively protected against cell death. The large-scale oscillations in ΔΨm observed after ~1 h of reperfusion were significantly delayed by 1 μM cyclosporin A and almost completely prevented by 50 μM PK11195 applied during 3 h of reperfusion. After an initial increase, mitochondrial Ca2+, measured with Myticam, rapidly declined during 3 h of reperfusion after the initial transient increase. This decline was prevented by application of PK11195 at the onset and during reperfusion. PK11195 prevented a significant increase in succinate utilization following I/R and succinate-induced forward-mode ROS generation. Treatment with PK11195 was also associated with a significant increase in glutamate and a decrease in leucine utilization.

Conclusion: PK11195 administered specifically at the moment of reperfusion limited ROS-induced ROS release and cell death, likely in part, by a shift from succinate to glutamate utilization. These data demonstrate a unique mechanism to limit cardiac injury after I/R.
Assessment of the Effect of Recruitment Maneuver on Lung Aeration Through Imaging Analysis in Invasively Ventilated Patients: A Systematic Review
Authors: Pierrakos Charalampos , Smit Marry R. , Hagens Laura A. , Heijnen Nanon F. L. , Hollmann Markus W. , Schultz Marcus J. , Paulus Frederique , Bos Lieuwe D. J.
Published: June 2021

Background: Recruitment maneuvers (RMs) have heterogeneous effects on lung aeration and have adverse side effects. We aimed to identify morphological, anatomical, and functional imaging characteristics that might be used to predict the RMs on lung aeration in invasively ventilated patients.

Methods: We performed a systemic review. Studies included invasively ventilated patients who received an RM and in whom re-aeration was examined with chest computed tomography (CT), electrical impedance tomography (EIT), and lung ultrasound (LUS) were included.

Results: Twenty studies were identified. Different types of RMs were applied. The amount of re-aerated lung tissue after an RM was highly variable between patients in all studies, irrespective of the used imaging technique and the type of patients (ARDS or non-ARDS). Imaging findings suggesting a non-focal morphology (i.e., radiologic findings consistent with attenuations with diffuse or patchy loss of aeration) were associated with higher likelihood of recruitment and lower chance of overdistention than a focal morphology (i.e., radiological findings suggestive of lobar or segmental loss of aeration). This was independent of the used imaging technique but only observed in patients with ARDS. In patients without ARDS, the results were inconclusive.

Conclusions: ARDS patients with imaging findings suggestive of non-focal morphology show most re-aeration of previously consolidated lung tissue after RMs. The role of imaging techniques in predicting the effect of RMs on re-aeration in patients without ARDS remains uncertain.
Identification of Transient Noise to Reduce False Detections in Screening for Atrial Fibrillation
Authors: Halvaei Hesam , Svennberg Emma , Sörnmo Leif , Stridh Martin
Published: June 2021

Screening for atrial fibrillation (AF) with a handheld device for recording the ECG is becoming increasingly popular. The poorer signal quality of such ECGs may lead to false detection of AF, often caused by transient noise. Consequently, the need for expert review in AF screening can become extensive. A convolutional neural network (CNN) is proposed for transient noise identification in AF detection. The network is trained using the events produced by a QRS detector, classified into either true beat detections or false detections. The CNN and a low-complexity AF detector are trained and tested using the StrokeStop I database, containing 30-s ECGs from mass screening for AF in the elderly population. Performance evaluation of the CNN-based quality control using a subset of the database resulted in sensitivity, specificity, and accuracy of 96.4, 96.9, and 96.9%, respectively. By inserting the CNN before the AF detector, the false AF detections were reduced by 22.5% without any loss in sensitivity. The results show that the number of recordings calling for expert review can be significantly reduced thanks to the identification of transient noise. The reduction of false AF detections is directly linked to the time and cost spent on expert review.
Soybean Nodulation Response to Cropping Interval and Inoculation in European Cropping Systems
Authors: Halwani Mosab , Reckling Moritz , Egamberdieva Dilfuza , Omari Richard Ansong , Bellingrath-Kimura Sonoko D. , Bachinger Johann , Bloch Ralf
Published: June 2021

To support the adaption of soybean [Glycine max(L) Merrill] cultivation across Central Europe, the availability of compatible soybean nodulating Bradyrhizobia (SNB) is essential. Little is known about the symbiotic potential of indigenous SNB in Central Europe and the interaction with an SNB inoculum from commercial products. The objective of this study was to quantify the capacity of indigenous and inoculated SNB strains on the symbiotic performance of soybean in a pot experiment, using soils with and without soybean history. Under controlled conditions in a growth chamber, the study focused on two main factors: a soybean cropping interval (time since the last soybean cultivation; SCI) and inoculation with commercial Bradyrhizobia strains. Comparing the two types of soil, without soybean history and with 1–4 years SCI, we found out that plants grown in soil with soybean history and without inoculation had significantly more root nodules and higher nitrogen content in the plant tissue. These parameters, along with the leghemoglobin content, were found to be a variable among soils with 1–4 years SCI and did not show a trend over the years. Inoculation in soil without soybean history showed a significant increase in a nodulation rate, leghemoglobin content, and soybean tissue nitrogen concentration. The study found that response to inoculation varied significantly as per locations in soil with previous soybean cultivation history. An inoculated soybean grown on loamy sandy soils from the location Müncheberg had significantly more nodules as well as higher green tissue nitrogen concentration compared with non-inoculated plants. No significant improvement in a nodulation rate and tissue nitrogen concentration was observed for an inoculated soybean grown on loamy sandy soils from the location Fehrow. These results suggest that introduced SNB strains remained viable in the soil and were still symbiotically competent for up to 4 years after soybean cultivation. However, the symbiotic performance of the SNB remaining in the soils was not sufficient in all cases and makes inoculation with commercial products necessary. The SNB strains found in the soil of Central Europe could also be promising candidates for the development of inoculants and already represent a contribution to the successful cultivation of soybeans in Central Europe.
Exploiting Epigenetic Variations for Crop Disease Resistance Improvement
Authors: Zhi Pengfei , Chang Cheng
Published: June 2021

Pathogen infections seriously threaten plant health and global crop production. Epigenetic processes such as DNA methylation, histone post-translational modifications, chromatin assembly and remodeling play important roles in transcriptional regulation of plant defense responses and could provide a new direction to drive breeding strategies for crop disease resistance improvement. Although past decades have seen unprecedented proceedings in understanding the epigenetic mechanism of plant defense response, most of these advances were derived from studies in model plants like Arabidopsis. In this review, we highlighted the recent epigenetic studies on crop-pathogen interactions and discussed the potentials, challenges, and strategies in exploiting epigenetic variations for crop disease resistance improvement.
The Stress and Anxiety to Viral Epidemics-6 Items (SAVE-6) Scale: A New Instrument for Assessing the Anxiety Response of General Population to the Viral Epidemic During the COVID-19 Pandemic
Authors: Chung Seockhoon , Ahn Myung Hee , Lee Sangha , Kang Solbi , Suh Sooyeon , Shin Yong-Wook
Published: June 2021

The general population has reported experiencing anxiety due to the COVID-19 pandemic. This study explored the validity and utility of the Stress and Anxiety to Viral Epidemics-6 items (SAVE-6) scale for measuring the anxiety response of the general population to the viral epidemic. About 1,009 respondents participated in an online survey. Of these, 501 (49.7%) participants were rated as having at least a mild degree of anxiety response to the viral epidemic (SAVE-6 score ≥ 15), while 90 (8.9%) and 69 (6.8%) participants were rated as having moderate degree of depression and anxiety, respectively. The SAVE-6 scale showed a good internal consistency (Cronbach’s alpha = 0.815). Parallel analysis suggested a one-factor structure for the measure. The SAVE-6 scale was found to be a reliable, valid, and useful brief measure that can be applied to the general population.
Neuropsychological Tests in Post-operative Cognitive Dysfunction: Methods and Applications
Authors: Liu Jun , Huang Kequn , Zhu Binbin , Zhou Bin , Ahmad Harb Ahmad Khaled , Liu Lin , Wu Xiang
Published: June 2021

Post-operative cognitive dysfunction (POCD) is a neurological complication that relatively frequently occurs in older people after anesthesia/surgery, with varying durations and significant differences in the severity of cognitive impairment. POCD is mainly characterized by memory loss mostly without consciousness disorders, accompanied by abnormal emotions, behaviors, and language, mostly without consciousness disorder. The clinical performance of POCD lacks specificity but can reflect the severity of cognitive impairment in patients. The diagnosis of POCD cannot be separated from the evaluation of perioperative cognitive function of patients, and the more popular and accepted method is neuropsychological tests (NPTs).
The Sexual Development, Sexual Health, Sexual Experiences, and Sexual Knowledge of Forensic Mental Health Patients: A Research Design and Methodology Protocol
Authors: Brand Elnike , Ratsch Angela , Heffernan Edward
Published: June 2021

Introduction: There is substantial evidence that severe mental illness (SMI) can have significant impacts on general development, knowledge acquisition, and quality of life including sexual function. However, whilst the sexual development, sexual health, sexual experiences and sexual knowledge of the broader Australian community and the Australian prisoner population have been well-described, these concepts have been less explored in people with a SMI. In particular, there is an absence of research around these topics involving people who are subject to a treatment order (aka a Forensic Order) under the relevant jurisdictional Mental Health Act.

Methods and Analysis: People currently under the treatment requirements of a Queensland Forensic Order will be invited to participate in this descriptive, mixed-method study. The study will be conducted in three phases. The first two phases will involve 50 participants (100 face-to-face quantitative interviews) with the aim of mapping sexual development, sexual health, sexual experiences, and sexual knowledge. The third phase will involve qualitative semi-structured interviews with a purposely enrolled, informant-rich cohort identified through the quantitative surveys until saturation is reached. Quantitative data will be analyzed using descriptive and comparative statistics. Qualitative data will be analyzed by content analysis of the major themes.

Ethics and dissemination: The project has ethics approval from a Queensland Health Human Research Ethics Committee. Results will be reported to participants and other stakeholders at seminars and conferences and through peer-reviewed publications.

Strengths and Limitations of this Study: This is a mixed-method study which engages with participants by using face-to-face interviews. The study is conducted in three phases which sub-divide the research into the following areas: (1) demographics, general health, and sexual health, (2) sexual knowledge and experiences, and (3) sexual knowledge gaps. This study uses electronic data capture to efficiently record and analyse participant responses. This study captures self-reported data and uses non-probability sampling from a population who have been pre-selected through an arms-length approach—both these processes elevate the risk of bias.
The Greater Houston Area Bipolar Registry—Clinical and Neurobiological Trajectories of Children and Adolescents With Bipolar Disorders and High-Risk Unaffected Offspring
Authors: Diaz Alexandre Paim , Cuellar Valeria A. , Vinson Elizabeth L. , Suchting Robert , Durkin Kathryn , Fernandes Brisa S. , Scaini Giselli , Kazimi Iram , Zunta-Soares Giovana B. , Quevedo João , Sanches Marsal , Soares Jair C.
Published: June 2021

The aims of this article are to discuss the rationale, design, and procedures of the Greater Houston Area Bipolar Registry (HBR), which aims at contributing to the effort involved in the investigation of neurobiological mechanisms underlying bipolar disorder (BD) as well as to identify clinical and neurobiological markers able to predict BD clinical course. The article will also briefly discuss examples of other initiatives that have made fundamental contributions to the field. This will be a longitudinal study with participants aged 6–17 at the time of enrollment. Participants will be required to meet diagnostic criteria for BD, or to be offspring of a parent with BD. We will also enroll healthy controls. Besides clinical information, which includes neurocognitive performance, participants will be asked to provide blood and saliva samples as well as to perform neuroimaging exams at baseline and follow-ups. Several studies point to the existence of genetic, inflammatory, and brain imaging alterations between individuals at higher genetic risk for BD compared with healthy controls. Longitudinal designs have shown high conversion rates to BD among high-risk offspring, with attempts to identify clinical predictors of disease onset, as well as clarifying the burden associated with environmental stressors. The HBR will help in the worldwide effort investigating the clinical course and neurobiological mechanisms of affected and high-risk children and adolescents with BD.
Sustainable Laboratory Capacity Building After the 2014 Ebola Outbreak in the Republic of Guinea
Authors: Ndjomou Jean , Shearrer Scott , Karlstrand Brendan , Asbun Carmen , Coble Jesse , Alam Jane S. , Mar Mar P. , Presser Lance , Poynter Scott , Michelotti Julia M. , Wauquier Nadia , Ross Casey , Altmann Sharon
Published: June 2021

Background: The 2014–2016 West Africa Ebola virus disease outbreak heavily impacted the Republics of Guinea, Sierra Leone, and Liberia. The outbreak uncovered the weaknesses of the public health systems, including inadequately trained and insufficient health personnel as well as limited and poorly equipped health infrastructures. These weaknesses represent significant threats to global health security. In the wake of the outbreak, affected countries made urgent requests for international engagement to help strengthening the public health systems.

Methods: This work describes the successful multi-year implementation of a laboratory capacity building program in the Republic of Guinea. The program integrated biorisk and quality management systems training, infectious diseases diagnostic training, facility engineering and maintenance training, and mentorship to strengthen Guinea's bio-surveillance capacity.

Results: The major outcome of these efforts was an established and local staff-operated public health laboratory that performs disease surveillance and reporting and diagnostic of priority diseases and pathogens of security concerns.

Conclusions: This work has improved the Guinea country's capabilities to address country public health issues and preparedness to respond to future infectious disease threats.
Emulation of Cosmological Mass Maps with Conditional Generative Adversarial Networks
Authors: Perraudin Nathanaël , Marcon Sandro , Lucchi Aurelien , Kacprzak Tomasz
Published: June 2021

Weak gravitational lensing mass maps play a crucial role in understanding the evolution of structures in the Universe and our ability to constrain cosmological models. The prediction of these mass maps is based on expensive N-body simulations, which can create a computational bottleneck for cosmological analyses. Simulation-based emulators of map summary statistics, such as the matter power spectrum and its covariance, are starting to play increasingly important role, as the analytical predictions are expected to reach their precision limits for upcoming experiments. Creating an emulator of the cosmological mass maps themselves, rather than their summary statistics, is a more challenging task. Modern deep generative models, such as Generative Adversarial Networks (GAN), have demonstrated their potential to achieve this goal. Most existing GAN approaches produce simulations for a fixed value of the cosmological parameters, which limits their practical applicability. We propose a novel conditional GAN model that is able to generate mass maps for any pair of matter density Ωm and matter clustering strength σ8, parameters which have the largest impact on the evolution of structures in the Universe, for a given source galaxy redshift distribution n(z). Our results show that our conditional GAN can interpolate efficiently within the space of simulated cosmologies, and generate maps anywhere inside this space with good visual quality high statistical accuracy. We perform an extensive quantitative comparison of the N-body and GAN -generated maps using a range of metrics: the pixel histograms, peak counts, power spectra, bispectra, Minkowski functionals, correlation matrices of the power spectra, the Multi-Scale Structural Similarity Index (MS-SSIM) and our equivalent of the Fréchet Inception Distance. We find a very good agreement on these metrics, with typical differences are <5% at the center of the simulation grid, and slightly worse for cosmologies at the grid edges. The agreement for the bispectrum is slightly worse, on the <20% level. This contribution is a step toward building emulators of mass maps directly, capturing both the cosmological signal and its variability. We make the code1 and the data2 publicly available.
In-Law Relationships in Evolutionary Perspective: The Good, the Bad, and the Ugly
Authors: Daly Martin , Perry Gretchen
Published: June 2021

In-laws (relatives by marriage) are true kin because the descendants that they have in common make them “vehicles” of one another’s inclusive fitness. From this shared interest flows cooperation and mutual valuation: the good side of in-law relationships. But there is also a bad side. Recent theoretical models err when they equate the inclusive fitness value of corresponding pairs of genetic and affinal (marital) relatives-brother and brother-in-law, daughter and daughter-in-law-partly because a genetic relative’s reproduction always replicates ego’s genes whereas reproduction by an affine may not, and partly because of distinct avenues for nepotism. Close genetic relatives compete, often fiercely, over familial property, but the main issues in conflict among marital relatives are different and diverse: fidelity and paternity, divorce and autonomy, and inclinations to invest in distinct natal kindreds. These conflicts can get ugly, even lethal. We present the results of a pilot study conducted in Bangladesh which suggests that heightened mortality arising from mother-in-law/daughter-in-law conflict may be a two-way street, and we urge others to replicate and extend these analyses.
Lameness in Beef Cattle: A Cross-Sectional Descriptive Survey of On-Farm Practices and Approaches
Authors: Tunstall Jay , Mueller Karin , Grove-White Dai , Oultram Joanne W. H. , Higgins Helen Mary
Published: June 2021

Cattle lameness is a concern to the United Kingdom (UK) cattle industry, negatively impacting upon welfare and production. Previous work involving one small study (n = 21) has identified that some UK beef farmers underestimate lameness prevalence, but also that farmers vary in their perception of the impact of lameness. Knowledge and skills of farmers were identified as a potential concern, and farmer-reported barriers were identified. However, the extent to which these views can be extrapolated is unknown. Therefore, the aim of this study was to produce descriptive results of UK beef farmer lameness-related activities concerning lameness identification, examination, treatment, and prevention. Questionnaires were circulated online and via post. Postal questionnaires were sent to registered Approved Finishing Units (a specific cohort of beef fattening units subject to strict biosecurity measures as part of UK bovine tuberculosis control) and a stratified sample of all registered beef enterprises in England and Wales. Online questionnaires were circulated on social media and via targeted emails asking selected industry bodies and veterinary practices to distribute to farmers. Descriptive results were produced, and thematic analysis was performed on free text responses. There were 532 usable responses, with most farmers self-reporting their current lameness prevalence as zero (mean 1.2%, range 0–20%). Most respondents did not locomotion score cattle, and most reported that it was not safe to examine feet. Most farmers did not use a foot bath, but of those who did, formaldehyde was the most commonly used product. Some farmers reported use of antibiotic foot baths. Most farmers reported dealing with lame animals within 48 h, but some only dealt with severe cases, and some felt that lame animals would get better by themselves. To deal with animals that have an ongoing lameness problem, transportation to slaughter was considered an option by 35% of farmers. It is worth noting, however, that the majority of lame animals would be precluded from transport under UK legislation. Farmers reported staff shortages, as well as a lack of time, training, and knowledge as barriers to lameness prevention and control. Overall, these results suggest that farmers may be underestimating lameness. Diagnosis is likely to be challenging, with unsafe facilities for lifting feet. The reported high threshold by some farmers for attending to a lame animal is a cause for concern, negatively impacting upon animal welfare, but this is also likely to have negative consequences for animal performance and farm profitability. Many participants in this study expressed a desire for farmer training in several aspects relating to lameness prevention and control, and this represents an opportunity for further knowledge exchange regarding lameness in beef cattle.
Risk Assessment of Human Consumption of Meat From Fenbendazole-Treated Pheasants
Authors: Carreño Gútiez Marta , Tell Lisa A. , Martínez-López Beatriz
Published: June 2021

Fenbendazole is a benzimidazole-class anthelmintic that is used for the control of immature and adult stages of internal parasites, such as nematodes and trematodes, in domestic food-animal species. It is not approved by the United States Food and Drug Administration for treating pheasants despite Syngamus trachea being one of the most prevalent nematodes that parasitize pheasants. Because it is a highly effective treatment, e.g., 90% effectiveness against S. trachea, and there are very few alternative therapeutic options, this anthelminthic is used in an extra-label manner in the pheasant industry, but few studies have been conducted assessing risks to humans. Therefore, we conducted a risk assessment to evaluate the potential repeat-dose and reproductive, teratogenic, and carcinogenic human risks that may be associated with the consumption of tissues from pheasants that were previously treated with fenbendazole. We conducted a quantitative risk assessment applying both deterministic and stochastic approaches using different fenbendazole sulfone residue limits (tolerance, maximum residue limits, and analytical limit of detection) established in different poultry species by the Food and Drug Administration, the European Medicines Agency, and other regulatory agencies in Japan, Turkey, and New Zealand. Our results show that fenbendazole poses minimal risk to humans when administered to pheasants in an extra-label manner, and a comparison of different fenbendazole sulfone residue limits can help assess how conservative the withdrawal interval should be after extra-label drug use.
Effects of Bacillus amyloliquefaciens Instead of Antibiotics on Growth Performance, Intestinal Health, and Intestinal Microbiota of Broilers
Authors: Wang Baikui , Zhou Yuanhao , Tang Li , Zeng Zihan , Gong Li , Wu Yanping , Li Wei-Fen
Published: June 2021

The aim of this study was to evaluate the dietary effects of Bacillus amyloliquefaciens SC06 (SC06) instead of antibiotics on the growth performance, intestinal health, and intestinal microbiota of broilers. A total of 360 30-day-old Lingnan yellow broilers were randomly allocated into two groups with six replicates per group (30 birds per replicate). The broilers were fed either a non-supplemented diet or a diet supplemented with 108 colony-forming units lyophilized SC06 per kilogram feed for 30 days. Results showed that SC06 supplementation had no effect on the growth performance compared with that of the control group. SC06 treatment significantly (P <0.05) increased the total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD) activity in the liver, and the activities of trypsin, α-amylase (AMS), and Na+K+-ATPase in the ileum, whereas it decreased (P < 0.05) lipase, gamma glutamyl transpeptidase (γ-GT), and maltase activities in the ileum. Meanwhile, SC06 treatment also improved the immune function indicated by the significantly (P < 0.05) increased anti-inflammatory cytokine [interleukin (IL)-10] level and the decreased (P < 0.05) pro-inflammatory cytokine [IL-6 and tumor necrosis factor (TNF)-α] levels in the ileum. Furthermore, we also found that SC06 enhanced the intestinal epithelial intercellular integrity (tight junction and adhesion belt) in the ileum. Microbial analysis showed that SC06 mainly increased the alpha diversity indices in the jejunum, ileum, and cecum. SC06 treatment also significantly (P < 0.05) increased the abundances of Bacteroidetes, Bacteroidales, Bacteroides, Fusobacteria, Clostridiaceae, and Veillonellaceae in the cecum and simultaneously decreased the abundances of Planococcaceae in the duodenum, Microbacteriaceae in the jejunum, and Lachnospiraceae, [Ruminococcus] and Ruminococcus in cecum. In conclusion, these results suggested that B. amyloliquefaciens instead of antibiotics showed a potential beneficial effect on the intestinal health of broilers.
The Effect of Manufacturing Quality on Rocket Precision
Authors: Trzun Zvonko , Vrdoljak Milan , Cajner Hrvoje
Published: June 2021

The effect of manufacturing quality on rocket impact point dispersion is analyzed. The approach presented here applies to any type of rocket. Here, manufacturing quality is demonstrated for the unguided rocket, and by simulating four typical manufacturing errors: erroneously manufactured warhead, misalignment between the warhead and engine chamber, asymmetrically installed propellant, and error in nozzle manufacturing. A new methodology is proposed, which combines a 3D CAD model of the asymmetrical projectile (due to manufacturing errors) and the improved Six-degrees-of-freedom (6DOF) model of its flight into a comprehensive Monte-Carlo simulation. In that way, the rocket trajectory dispersion is correlated directly to the imperfection of the manufacturing process. Three quality levels are simulated (low, standard, and high quality), and each of the analyzed manufacturing errors depends on the chosen quality. The results show how important it is to impose the highest quality on nozzle manufacturing, and if this condition is not met, reveal if strict tolerances applied to other steps of the manufacturing process can compensate for the consequential drop of precision.
Impact of Different Water Management Regimes on the Growth, Productivity, and Resource Use Efficiency of Dry Direct Seeded Rice in Central Punjab-Pakistan
Authors: Hussain Sadam , Hussain Saddam , Aslam Zubair , Rafiq Muhammad , Abbas Adeel , Saqib Muhammad , Rauf Abdur , Hano Christophe , El-Esawi Mohamed A.
Published: June 2021

Dry direct-seeded rice has been shown to save irrigation water and labor. Nonetheless, irrigation management in dry direct-seeded rice has received very little attention. Here, we examined the potential of different irrigation regimes: aerobic rice (AR), alternate wetting and drying (AWD) and continuous flooding (CF) in dry direct-seeded rice cultivation on two rice cultivars (Pride-1 (hybrid indica) and NB-1 (inbred indica)). Growth, yield attributes, grain yield, total water input, water productivity and benefit cost ratio were measured. Our results showed that AR saved 11.22 and 28.40%, and 5.72 and 32.98% water compared with AWD and CF during 2018 and 2020, respectively. There was a significant difference in grain yield among treatments and cultivars. AWD and CF produced statistically same total dry weight and grain yield, while AR reduced the total dry weight by 31.34% and 38.04% and grain yield by 34.82% and 38.16% in comparison to AWD and CF, respectively, across the years. Except for 1000-grain weight and harvest index in AWD and CF, further differences in total dry weight and grain yield among irrigation treatments were primarily correlated with variations in yield attributes. Among the cultivars, hybrid rice performed better than inbred rice. Over the two-year period, hybrid rice increased total dry weight, grain yield, and water productivity by 9.28%, 13.05%, and 14.28%, respectively, as compared to inbred rice. Regarding water productivity (WP), the maximum percentage (40.90 and 26.53%) was recorded for AWD compared to AR and CF. Among cultivars, more water productivity (14.28%) was calculated for hybrid rice than inbred one. Chlorophyll and carotenoid contents, leaf area index and crop growth rate contributed to higher grain yield of hybrid rice under AWD and CF. In contrast to WP, the maximum benefit cost ratio was estimated to be higher for CF than that of AR and AWD. For the cultivars, the maximum value (2.26 in 2018 and 2.32 in 2020) was calculated for hybrid rice compared with the inbred one. In conclusion, these results suggests that AWD with maximum WP and CF with maximum BCR could be more efficient approaches than AR. Under CF, hybrid rice cultivars with higher yield and yield-related attributes, WP and BCR performed better.
Genotypic and Environmental Effect on the Concentration of Phytochemical Contents of Lentil (Lens culinaris L.)
Authors: Irakli Maria , Kargiotidou Anastasia , Tigka Evangelia , Beslemes Dimitrios , Fournomiti Maria , Pankou Chrysanthi , Stavroula Kostoula , Tsivelika Nektaria , Vlachostergios Dimitrios N.
Published: June 2021

The health-promoting effects of lentil seeds due to phenolic compounds and other antioxidants make lentils a potential source of functional food or feed ingredients. The objective of this study was to evaluate the effects of genotype and growing environment on the phytochemical contents and antioxidant activities such as ABTS (2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) assays of soluble extracts from five lentil cultivars grown in ten diverse locations over a 2-year experimental period. Total phenolic content (TPC), total flavonoid content (TFC), total proanthocyanidin content (TPAC), total hydrolyzed tannin content (TNC), tocopherols and carotenoids were investigated. The major proanthocyanidins and individual polyphenols were quantified by high-performance liquid chromatography. Our results indicated that flavanols were the main phenolic compounds in hydrophilic extracts, followed by phenolic acids. Concerning lipophilic extracts, tocopherols and carotenoids were the main components, with γ-tocopherol and lutein being the predominant isomers, respectively. In general, both genetic and environmental effects had a strong impact on all bioactive components tested. Greater variation due to environmental effects was found for phenolic compounds (TPC, TFC and TPAC) and antioxidant activities; however, tocopherols and carotenoids revealed a high genotypic dependence. The principal component analysis highlighted the genotypes with higher content of antioxidants and stability across environments. The red lentil population “03-24L” was characterized as a promising genetic material due to its high phenolic contents and antioxidant capacity values across environments and is suggested for further investigation. In conclusion, multi-environmental trials are essential for a better understanding of the genotypic and environmental effect on phytochemical profiles of lentils and provide important information for breeding or cultivating lentil varieties of high-bioactive value.
Energy Communities Design Optimization in the Italian Framework
Authors: Zatti Matteo , Moncecchi Matteo , Gabba Marco , Chiesa Alberto , Bovera Filippo , Merlo Marco
Published: June 2021

Energy communities (EC) are expected to have a pivotal role to reach European decarbonization targets. One of the key aspects is the regulatory framework adopted by each Member State to properly manage such new customers’ aggregation. The paper firstly provides an updated overview of the EC regulation, focusing on the current Italian legislation. Next, a novel methodology for the design and management of energy community initiatives is proposed. The procedure firstly solves a design and operation optimization problem to calculate the best size of energy assets (boiler, heat pump, photovoltaic, thermal storage) to be installed. Second, a Shapley value-based approach is exploited to distribute a part of the community’s incomes to members, based on their contribution to the overall welfare. Results demonstrate that the adopted methodology is effective in ensuring a proper cash flow for the community, while pushing its members towards energy efficient behaviors.
Mixed Mode I/II Fracture Analysis of Bi-Material Adhesive Bonded Joints Using a Novel Short Beam Specimen
Authors: Aliha Mohammad Reza Mohammad , Kucheki Hadi Ghesmati , Mirsayar Mirmilad
Published: June 2021

Until now, some test specimens with different shapes and loading mechanisms have been utilized for investigating the cracking behavior of adhesive bounded joints. In this research, using a novel test configuration called adhesive short bend beam specimen containing an inclined crack and loaded by three-point bending, mixed mode I/II fracture parameters of a crack initiated in the adhesive part is studied. Compared to other test methods, the specimen used in this research needs a lesser amount of material and the fracture test can be performed easily. A large number of finite element models of this specimen were analyzed using ABAQUS code to study the effect of adhesive and adherent type, and also the crack length and loading span distance on KI, KII, T-stress and fracture initiation direction under different mode mixities. The results showed that the fracture parameters (and in particular the shear mode component) are sensitive to the type and location of adherent in the bounded joint; however, the shape and size of fracture plastic zone is not affected noticeably by the type of adhesive-adherent materials. It was also shown that the complete mode mixities ranging from pure mode I to pure mode II can be introduced for adhesive bounded joints using the proposed test specimen and therefore the specimen is a good candidate test configuration for investigating the mixed mode I/II fracture behavior of adhesive bounded joints.
Curiosities about Thermal Indices Estimation and Application
Authors: Matzarakis Andreas
Published: June 2021

To date, it is well known that the effect of heat or cold on human beings cannot be described and quantified based only on one single meteorological or thermo-physiological parameter [...]
The Temporal and Spatial Changes of Ship-Contributed PM2.5 Due to the Inter-Annual Meteorological Variation in Yangtze River Delta, China
Authors: Chen Dongsheng , Liang Dingyue , Li Lei , Guo Xiurui , Lang Jianlei , Zhou Ying
Published: June 2021

Ship-exhausted air pollutants could cause negative impacts on air quality, climate change, and human health. Increasing attention has been paid to investigate the impact of ship emissions on air quality. However, the conclusions are often based on a specific year, the extent to which the inter-annual variation in meteorological conditions affects the contribution is not yet fully addressed. Therefore, in this study, the Weather Research and Forecast model and the Community Multiscale Air Quality model(WRF/CMAQ) were employed to investigate the inter-annual variations in ship-contributed PM2.5 from 2010 to 2019. The Yangtze River Delta (YRD) region in China was selected as the target study area. To highlight the impact of inter-annual meteorological variations, the emission inventory and model configurations were kept the same for the 10-year simulation. We found that: (1) inter-annual meteorological variation had an evident impact on the ship-contributed PM2.5 in most coastal cities around YRD. Taking Shanghai as an example, the contribution varied between 3.05 and 5.74 µg/m3, with the fluctuation rate of ~65%; (2) the inter-annual changes in ship’s contribution showed a trend of almost simultaneous increase and decrease for most cities, which indicates that the impact of inter-annual meteorological variation was more regional than local; (3) the inter-annual changes in the northern part of YRD were significantly higher than those in the south; (4) the most significant inter-annual changes were found in summer, followed by spring, fall and winter.
Effect of Aging Vessel (Clay-Tinaja versus Oak Barrel) on the Volatile Composition, Descriptive Sensory Profile, and Consumer Acceptance of Red Wine
Authors: Issa-Issa Hanán , Lipan Leontina , Cano-Lamadrid Marina , Nemś Agnieszka , Corell Mireia , Calatayud-García Pablo , Carbonell-Barrachina Ángel A. , López-Lluch David
Published: June 2021

Consumers look for unique wines, offering pleasant experiences. Wine producers need to open new markets and are targeting countries with fewer traditions in drinking red and complex wines, such as Poland, Russia and Germany. The use of less popular aging vessels (e.g., clay-tinajas) will help in creating unique wines. The aim of this study was to evaluate the effect of the aging vessel on the volatile and sensory profiles and consumer acceptance of red wine in Spain and Poland (model of potential new markets). Three wines were studied: (i) wine A, aged in a clay-tinaja with non-permeable coating); (ii) wine B, aged in clay-tinaja without coating; and (iii) wine C, aged in oak barrels (control). The key families in the volatile profiles were esters (wines B and C) and organic acids and terpenes (wine A). Wine A was described as sour and bitter, wine B had a distinctive mineral note, and wine C had a complex profile with typical wood notes. Finally, wines C and A were the preferred ones for Spanish and Polish consumers, respectively. Clay-tinaja wine A can be a good option to introduce clay-tinaja wines in Polish and similar markets because it is a unique product and fulfills the sensory demands/habits of Polish consumers.
Different Microfluidic Environments for In Vitro Testing of Lipid Nanoparticles against Osteosarcoma
Authors: Mitxelena-Iribarren Oihane , Lizarbe-Sancha Sara , Campisi Jay , Arana Sergio , Mujika Maite
Published: June 2021

The use of lipid nanoparticles as biodegradable shells for controlled drug delivery shows promise as a more effective and targeted tumor treatment than traditional treatment methods. Although the combination of target therapy with nanotechnology created new hope for cancer treatment, methodological issues during in vitro validation of nanovehicles slowed their application. In the current work, the effect of methotrexate (MTX) encapsulated in different matrices was evaluated in a dynamic microfluidic platform. Effects on the viability of osteosarcoma cells in the presence of recirculation of cell media, free MTX and two types of blank and drug-containing nanoparticles were successfully assessed in different tumor-mimicking microenvironments. Encapsulated MTX was more effective than the equal dose free drug treatment, as cell death significantly increased under the recirculation of both types of drug-loaded nanoparticles in all concentrations. In fact, MTX-nanoparticles reduced cell population 50 times more than the free drug when 150-µM drug dose was recirculated. Moreover, when compared to the equivalent free drug dose recirculation, cell number was reduced 60 and 100 points more under recirculation of each nanoparticle with a 15-µM drug concentration. Thus, the results obtained with the microfluidic model present MTX-lipid nanoparticles as a promising and more effective therapy for pediatric osteosarcoma treatment than current treatment options.