bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Auxin Transport Inhibition on the Formation of Various Leaf and Vein Patterns
Authors: Wenzel Carol , Holloway David , Mattsson Jim
Published: August 2024

Polar auxin transport (PAT) and auxin efflux carriers are known components controlling leaf complexity and venation patterns in some model plant species. Evidence indicates that PAT generates auxin converge points (CPs) that in turn lead to local leaf formation and internally into major vein formation. However, the role of PAT in more diverse leaf arrangements and vein patterns is largely unknown. We used pharmacological inhibition of PAT in developing pinnate tomato, trifoliate clover, palmate lupin, and bipinnate carrot leaves and observed dosage-dependent reduction to simple leaves in these eudicots. Leaf venation patterns changed from craspedodromous (clover, carrot), semi-craspedodromous (tomato) and brochidodromous (lupin) to more parallel patterning with PAT inhibition. The visualization of auxin responses in transgenic tomato plants showed that discrete and separate CPs in control plants were replaced by diffuse convergence areas near the margin. These effects indicate that PAT plays a universal role in the formation of different leaf and vein patterns in eudicot species via a mechanism that depends on the generation as well as separation of auxin CPs. Computer simulations indicate that variations in PAT can alter the number of CPs, corresponding leaf lobe formation and position of major leaf veins along the leaf margin in support of experimental results.
Prediction of Environmental Parameters for Predatory Mite Cultivation Based on Temporal Feature Clustering
Authors: Ma Ying , Lin Hongjie , Chen Wei , Chen Weijie , Wang Qianyan
Published: August 2024

With the significant annual increase in the market demand for biopesticides, the industrial production demand for predatory mites, which hold the largest market share among biopesticides, has also been rising.To achieve efficient and low energy consumption control of predatory mite breeding environmental parameters, accurate estimation of breeding environmental parameters is necessary. This paper collects and pre-processing hourly time-series data of temperature and humidity from industrial breeding environments. Time series prediction models such as SVR, LSTM, GRU, and LSTNet are applied to model and predict historical data of the breeding environment. Experiments validate that the LSTNet model is more suitable for such environmental modeling. To further improve prediction accuracy, the training data for the LSTNet model is enhanced using hierarchical clustering of time-series features. After augmentation, the root mean square error (RMSE) of temperature prediction decreased by 27.3%, and the RMSE of humidity prediction decreased by 32.8%, significantly improving the accuracy of multi-step predictions and providing substantial industrial application value.
Pengaruh Moral, UU Harmonisasi Peraturan Perpajakan, dan Kondisi Keuangan Terhadap Kepatuhan Wajib Pajak UMKM
Authors: Nur Alvi Syahrina  , Hasanah Nuramalia , Yusuf Muhammad
Published: August 2024

The purpose of this study is to analyze the effect of morals, tax harmonization laws, and financial status on the tax compliance of MSE taxpayers. This research is quantitative research and data analysis in the form of descriptive statistical analysis using IBM SPSS Statistics 25 software and multiple regression analysis on Microsoft Excel. In this study, distributed questionnaires containing questions are used as main data. to MSE owners in South Jakarta. To select a population sample, researchers used the 100 persons make up the sample size for this convenience sampling technique. The outcomes revealed that morale and harmonization of tax laws have a positive effect on MSE tax compliance, while financial status has a negative effect on MSE tax compliance. Future researchers are advised to conduct research with additional variables that might increase the impact on taxpayer tax compliance and with more supporting data in the form of both primary and secondary data.
Science Program Enhancement for Teacher at Indonesian School Overseas
Authors: Delina Mutia , Madani Faisal , Sri Rahayu  , Yuyus Kardiman
Published: August 2024

Indonesian School Overseas applies Indonesia’s curricula. This community service was conducted onsite in three Indonesian schools overseas:  Bangkok (Thailand), Johor Bahru (Malaysia), and Singapura (Singapore). The community services program aims to enhance teachers’ knowledge and skills in science activities with simple tools in daily life. Besides onsite, the program was also conducted online with three keynote speakers from Murdoch University, Australia, Curtin University, Australia, and Universiti Teknologi Malaysia, Malaysia. The Program was attended by 46 onsite participants, and 146 online participants.
The depiction of sexism experienced by female characters Elizabeth Zott in Lessons in Chemistry (2023) Series
Authors: Mirani Indar , Elve Oktaviani
Published: August 2024

This study employs the sexism theory developed by Peter Glick and Susan Fiske to examine the portrayal of sexism in the 1950s through the character of Elizabeth Zott in the series Lessons in Chemistry (2023). The research method is qualitative with a descriptive approach. This research shows that Elizabeth Zott encounters two forms of sexism in a male-dominated workplace: hostile sexism, which includes direct hostility and discrimination, and benevolent sexism, which is more subtle and paternalistic but still excludes women from equal opportunities. Elizabeth is a woman who boldly rejects the traditional roles expected by a patriarchal society and confidently asserts her independence as an individual, despite social pressures. In the face of hostile sexism, Elizabeth is determined and courageous, continuing her career in science despite facing direct discrimination and challenges. She also challenges social expectations that limit women's roles and demands change for gender equality in the face of benevolent sexism. This study highlights Elizabeth's resistance strategies against both forms of sexism and the importance of understanding and addressing various forms of sexism in a male-dominated workplace.
 
Keywords: sexism, workplace, hostile sexism, benevolent sexism, traditional roles
The value of women's body signs in Maman Suherman's novel Re dan Perempuan
Authors: Suarni Syam Saguni
Published: August 2024

This study aims to analyze and discover the value of body signs in Maman Suherman's novel titled “Re dan Perempuan (2021)” through a postmodernism lens. This qualitative research aims to describe the meaning behind the meaning, as a phenomenon regarding the value of women's body signs in Maman Suherman's novels based on Jean Baudrillard's postmodernism theory. The results show the depiction of sexual behavior as a phenomenon in urban society. This behavior is part of the libido system where individuals engage in passive, weak, and powerless activities, even towards their own bodies. Essentially, in the postmodernism era, the value of these body signs is the forms of bodies driven by subjects, whether capitalism or patriarchy, that have rooted in certain societies.

Keywords: body signs, gender consumerism, Indonesian literature, postmodernism
Student Teachers Readiness and Challenges for Internship Program: A Survey on English Language Education Study Program, Universitas Negeri Jakarta
Authors: Fadhilah Farah Shiba , Mayuni Ilza , Lasito Lasito
Published: August 2024

For students majoring in education, the teaching internshipprogram is mandatory. Along with employing the KurikulumMerdeka in schools, student teachers must also prepare to useit through the teaching internship program. This study aims toinvestigate student teachers’ readiness and challenges for theteaching internship program. This study employs bothquantitative and qualitative methodologies to achieve itsobjectives. In all, 40 English Language Education StudyProgram student teachers batch 2020 at Universitas NegeriJakarta completed questionnaires and participated in FocusedGroup Discussions focusing on their readiness and challengesbased on key teaching competences, enabling competences,professional conduct, and personality. The research uncoveredstudent teachers level of readiness and challenges in'administration', 'methodology: knowledge and skills','assessment', and 'lesson and course preparation' to KurikulumMerdeka as ‘developing readiness’ and ‘mostly challenging’.However, they perceived ‘approaching readiness’ and viewsthem as ‘mostly not challenging’ aspects as in 'digital mediaimplementation,' 'professional behavior', 'personality', and'intercultural skills'. Because prior studies did not cover theseareas all at once, the knowledge obtained through this researchstudy applies locally.
A Systematic Review of Research on Blended Learning in Teaching EFL: Student and Teacher’s Perception, Implementation, Interaction, Benefits, and Challenges
Authors: Koniah Musfi Kotul , Putri Rizkia Aura , Anindita Shafinaz Aulia , Masayu Zakia Reeva
Published: August 2024

This study examines the existing research on blended learning inEnglish as a Foreign Language (EFL) classrooms, focusing on theviews and experiences of both students and teachers. An analysisof 30 published articles reveals that researchers have employed arange of methods, including quantitative, qualitative, and mixedapproaches, to collect data through questionnaires, interviews,and literature reviews. The findings suggest that both students andteachers have a positive outlook on blended learning, which is usedto teach various English skills and involves the use of onlineplatforms to assign different types of tasks. Effective interactionbetween students, teachers, and peers is crucial to the success ofblended learning. The benefits of blended learning includeimproved English proficiency, enhanced learning behaviors, anddeveloped digital skills. However, challenges such as technicaldifficulties, inadequate training, time management issues, andlearner preparedness can hinder its implementation.
Alain Thomas, le linguiste face au déclin des identités périphériques
Authors: Paré François
Published: August 2024

Alain Thomas appartient à une importante génération de chercheurs qui, dès le milieu des années 1980, tente de témoigner, par la conduite d’enquêtes sur le terrain, de l’évolution des langues minoritaires et des parlers régionaux tant au Canada qu’ailleurs dans le monde. Ce chapitre s’intéresse aux travaux de Thomas dans ces années 1990-2010 où le linguiste semble de plus en plus préoccupé par le déclin du français en Ontario. Alors qu’il s’était largement rallié aux conclusions de Raymond Mougeon sur la stabilité relative des langues en contact, celui-ci s’inquiète de plus en plus de l’avenir des différences linguistiques face à la domination de l’anglais. Ses écrits, durant ces années où les contextes sociolinguistiques évoluent, révèlent, par-delà l’analyse méticuleuse des données recueillies, le souci croissant du chercheur, plus que jamais attentif aux communautés qu’il étudie, de rendre compte des mécanismes déstructurants qui contribuent au dépérissement éventuel de la francophonie canadienne.
Workplace Conflict Effect on Innovative Behavior: The Roles of Engagement and Proactive Personality
Authors: Yelgin Çetin , Geylan Aslı
Published: August 2024

Conflict is as natural a phenomenon as harmony in social relations. In all industries, especially those prioritizing collaborative teamwork, it is clear that effective conflict management is critical to sustaining positive employee relationships. With this study, we set out to investigate the impact of workplace conflicts on employee innovation. For this purpose, data were collected from aviation industry employees through a survey, and 393 responses were received. Structural equation modeling was used to test hypotheses regarding the direct and indirect effects of conflict with colleagues on innovative behaviors. The study revealed that conflict with colleagues has a detrimental impact on both innovative behaviors and work engagement. Work engagement was identified as a completely mediator in the relationship between conflict and innovative behaviors. Furthermore, proactive personality was found to moderate the influence of conflict on innovative behaviors, also playing a significant role in the indirect relationship among these variables. The research concludes with practical recommendations and suggestions for future studies.
Computational and Experimental Study of the Patterns of Formation of a Fluidized Bed of Inert Haydite Particles Material
Authors: Mitrofanov A. V. , Vasilevich S. V. , Stojko S. V.
Published: August 2024

At present, an actual trend in the development of fuel and energy complexes in a number of countries is the diversification of generation via the involvement of local types of solid fuel. In this case, thermochemical processing of fuel is often carried out in a fluidized state. A significant proportion of dispersed fuels cannot be transferred to a state of stable fluidization. The solution in such cases is to create a fluidized bed of inert carrier, into which particles of the target fuel component are then introduced. In this work, a computational and experimental study of the fluidization of inert bulk material (haydite granules) was carried out. The key purpose of the work was to develop a mathematical model for the formation of a fluidized bed, which makes it possible to calculate the process based on its local characteristics, as well as to identify the model parameters and test it using experiment data. During the study the problems of developing a numerical method for calculating the distribution of velocities and concentrations along the height of the apparatus were solved, parametric identification of the proposed mathematical model was carried out, and empirical verification of the modeling results was carried out. The mathematical apparatus of the theory of Markov chains was used as the mathematical basis for constructing the model. The data from our own laboratory full-scale experiment were used to identify the parameters of the model and verify it. A comparison of calculated and experimental data showed the high predictive efficiency of the model for the given granulometric composition of the fluidizing product. The results of the laboratory full-scale experiments also showed a significant evolution of the granulometric composition of haydite granules during their long-term stay in a fluidized bed, which requires a separate study, as well as the introduction of appropriate amendments to the mathematical model for its further improvement.
Spontaneous Overgrowth of Jointing in the Working Chambers of Power Gas-Liquid Thermal Installations – Diffusion Dynamic Processes
Authors: Ryzhova T. V. , Bukharov D. N. , Arakelian M. M. , Arakelian S. M.
Published: August 2024

The issues of the formation of inhomogeneities and cracks as well as the effects of their spontaneous healing are considered on the well-known principles and concepts of nonlinear dynamics and quantum technologies in order to fully describe the picture of possible processes of the genesis of heterogeneities and their self-organization. At the same time, the process of spontaneous overgrowth of micro-inhomogeneities in solid-state/metal products, such defects in which occur under different operating conditions, is discussed on the example of thermal power plants of various types with cyclic processes and vibrations. The mechanism of such healing is determined by dynamic processes of unsteady diffusion with certain temporal characteristics evaluated within the framework of the corresponding model concepts. A model of diffusion-limited aggregation of defects/particles implemented by the cellular automaton method from the Neumann neighborhood, a model of random and ballistic deposition, as well as a percolation approach are considered. The process of modification and development of a 3D fractured structure based on the Griffiths load theory for dislocations is described, taking into account the gradual sequential transition from a stable state to a growth stage. As a result of the action of such dynamic loads in a solidstate object, a rapid change in the parameters of microcracking is observed, which can be represented as local micro-explosions, viz. the growth and fusion of natural microcracks into larger ones; the emergence of new microcracks; the disclosure of large microcracks with the formation of defects of the next hierarchical level. In the overview aspect, the universal modes of operation of a nonlinear dynamic system, well-known in mathematics and physics and suitable for analyzing the stability and sustainability of thermal power plants, are considered. The ongoing processes are associated with different types and strategies for the development of heterogeneities, such as: collapse and stagnation; stable periodicity; parameters “at odds”; chaotic development within certain limits; disruption/sudden crisis; a sharp leap and a breakthrough in development. The proposed approaches can be useful in improving the real operational condition of chambers with working matter in power plants when they operate in various operating modes.
Generalized Complex Variables of Drying Kinetics in Calculations of Drying Duration of Flat Thin Wet Materials
Authors: Ol’shanskii A. I. , Golubev A. N.
Published: August 2024

The article is devoted to the processing of experimental data regarding the drying of thin wet materials. The calculated kinetic dependencies necessary to determine the drying duration can be obtained from solving differential mass transfer equations, but this task is analytically complex, since drying is a non-stationary process, and the transfer coefficients depend on the moisture content and temperature of the material in a very complex form. This necessitates experimental studies with the development of approximate simple empirical equations for determining the drying duration with a minimum number of constants determined from the experiment. When processing the experiment, the relative drying rate, generalized drying time, the ratio of drying time by periods and the ratio of the current moisture content to the critical one were accepted as stable complex generalized variables when processing the experiment. All variables are interrelated by the drying rate in the first period (the period of constant rate). As a result of processing experimental data on convective drying of ceramics, asbestos, felt, formulas were obtained that make it possible to determine its duration in the period of falling rate. The invariance caused by such experimental processing allows one to move from one variable to another, from one coordinate system to another, which significantly reduces the number of necessary experiments. Data processing and analysis of the resulting formulas shows that the drying curves are complex exponentials, and the main mediating variables are the relative drying rate and relative moisture content. Verification of the accuracy of experimental data processing and the reliability of the obtained empirical equations, which make it possible to calculate the drying duration for the specified materials, is presented. The spread of the calculated values compared to the experiment is about 10 %, which is within the accuracy of experimental data processing.
A Novel Approach to Extract Cross-Organization Collaborative Process Models from Emergency Plans of Natural Disaster
Authors: Guo Wenyan , Yang Qingtao , Zeng Qingtian , Duan Hua , Ni Weijian
Published: August 2024

Emergency response of natural disaster is a complex process involving multiple response levels and cross-organization collaboration. Traditionally, cross-organization collaborative process models of emergency response are constructed based on the common experiences of domain experts and business process modelers. Textual emergency plans are not conducive to emergency performers quickly understanding response tasks and managers analyzing and evaluating the quality of emergency plans of natural disaster. In this paper, a novel approach is proposed to automatically extract cross-organization collaborative process models from emergency plans for supporting emergency response process modeling and the automation analysis of emergency plans. First, an attention mechanism is introduced into the Bi-directional Long Short-Term Memory network combined with a Conditional Random Field (BiLSTM-Attention-CRF), which is trained to identify process elements from sentences. Second, inter-organization relationships are extracted to generate a cross-organization collaborative network of emergency response. Then, response tasks and task relationships are extracted to generate emergency response sub-process models for all organizations. Finally, inter-organization relationships integrate all sub-process models into a global cross-organization collaborative process model of emergency response. A real-world dataset is collected for experimental evaluation and the results illustrate that the proposed approach is capable of extracting automatically cross-organization collaborative process models from emergency plans of natural disaster, and the automatically extracted models are highly consistent with the manually extracted models.
Genetically Engineered Mouse Models Unveil Mechanisms and Therapeutic Strategies for GMPPB-Associated Dystroglycanopathy
Authors: Wang Yuxiang , fu Ziwei , Wang Wangtong , Chen Yanyan , Zhang Chenyang , Yang Ju , Yang Hua , Yan Bing , Gong Baoming , Lu Weiqiao , Liu Ying , Sun Lei , Jiang Hao , Zhang Zhao , Chen Bo , Liu Xiuping
Published: August 2024

The pathogenesis and therapeutic avenues for GDP-mannose pyrophosphorylase
B (GMPPB)-associated dystroglycanopathy (DPG) have not been comprehensively
characterized. Recent studies have highlighted a significant association between
mutations in GMPPB with DGP, a rare disease characterized by neuromuscular
phenotypes. To interrogate the molecular mechanisms driving the onset and progression
and to identify potential therapeutic approaches for GMPPB-associated DGP, we here
constructed genetically engineered mice models of Gmppb. We show that Gmppb
knockout and P32L mutant mice are lethal in the homozygous form, whereas
homologous R287Q mutants are viable. Heterozygous Gmppb-P32L mutant mice
exhibit reduced muscle strength, decreased locomotor ability, elevated creatine kinase
levels and increased centrally nucleated myofibers. Furthermore, loss of GMPPB
results in defective differentiation of muscle stem cells, leading to failure to develop
into mature myotubes and diminished muscle regeneration capability. Biochemical and
transcriptomic analyses indicate that loss of GMPPB is associated with significant
alterations in protein glycosylation, intracellular Ca2+ storage and release, and the
Wnt/β-catenin signaling pathway. Pharmacological activation of the Wnt pathway
alleviated disruption of muscle differentiation and regeneration post muscle injury in
Gmppb-deficient models. Additionally, adeno-associated virus (AAV)-mediated gene
replacement therapy successfully ameliorated the muscular phenotypes. Collectively,
our findings provide direct evidence that impaired muscle stem cell differentiation
contributes to GMPPB-associated dystroglycanopathy. Wnt pathway agonists and AAV
gene therapy represent potential effective intervention strategies for treating DGP
disease.
Von Hippel-Lindau Disease Presenting with Renal Cell Carcinoma and Multiple Organ Involvement: A Case Report
Authors: Hashemi Peyman , Aalinezhad Marzieh , Shahsavan Mahdi , Naderi Yosra
Published: August 2024

Introduction:
Von Hippel-Lindau (VHL) disease is a rare autosomal dominant disorder characterized by the development of both neoplastic and cystic lesions in multiple organs, including the central nervous system (CNS), retina, kidneys, pancreas, and adrenal glands. Early detection and careful management are critical due to the potential for significant morbidity and mortality associated with this condition.
Case Presentation:
A 37-year-old man with a history of VHL presented with abdominal discomfort and hematuria. His medical history included surgical resection of a cerebellar hemangioblastoma 20 years ago and a retinal capillary hemangioma diagnosed 5 years ago. Genetic testing confirmed the presence of a pathogenic VHL variant. Abdominopelvic multidetector computed tomography revealed multiple pancreatic cysts, renal cysts, two enhancing renal lesions suggestive of renal cell carcinoma (RCC), and a retroperitoneal mass indicative of extra-adrenal paraganglioma. Biopsy confirmed the presence of clear cell RCC. The patient exhibited classic manifestations of VHL, including central nervous system hemangioblastomas, retinal hemangiomas, pancreatic cysts, RCC, and extra-adrenal paragangliomas.
Conclusions
This case highlights the diverse manifestations of VHL disease, including CNS hemangioblastomas, retinal hemangiomas, pancreatic cysts, RCC, and paragangliomas. The patient's comprehensive clinical, imaging, and histopathological evaluations met the diagnostic criteria for VHL. Regular screening and close follow-up are crucial to detect complications early, allowing for timely intervention and improved outcomes.
Ongoing intensification of El Niño impacts on East Asia's summer climate with achieved carbon neutrality
Authors: Tao Weichen , Zhang Wen , Huang Gang , Hu Kaiming , Qu Xia , Wang Ya , Tang Haosu , Zhang Suqin
Published: August 2024

A notable anomalous lower-tropospheric anticyclone appears over the western North Pacific (WNP) during post-El Niño summer, exerting a profound influence on the East Asia summertime climate. Here, we employ a suite of global climate model projections under symmetric CO2 ramp-up (RU) and ramp-down (RD) scenarios to reveal the asymmetric response of the WNP anticyclone (WNPAC). Our results demonstrate a progressive strengthening of the WNPAC with rising CO2 concentrations, a trend that persists as CO2 declines, followed by gradual recovery, and the anomalous anticyclone fails to return to its initial state when CO2 concentrations return to pre-industrial levels, attributed to Indo-Pacific zonal SST gradient variations. In contrast to the CO2 RU phase, the increased zonal SST gradient is witnessed in the CO2 RD phase, favoring anomalous moisture convergence over the Maritime Continent (MC). This strengthens the WNPAC through Kelvin wave-induced Ekman divergence or local Hadley circulation adjustment. The increased zonal SST gradient is associated with strengthened SST warming in the MC and accelerated decay of El Niño-related positive SST anomalies in the equatorial Central Pacific, ultimately attributed to a climatological equatorial Pacific El Niño-like warming pattern driving intricate air-sea interactions and processes. Our findings indicate that the overshoot of the WNPAC during the CO2 RD phase may exacerbate the flood and high temperature risks in densely populated East Asia.
Design and Analysis of Electrical characteristic of Non-contact Capacitive Electrode for ECG Application
Authors: S Ramya Lakshmi V , Raajan N R
Published: August 2024

Purpose: Capacitive-based sensors have become prominent in physiological sig- nal measurement over the past decade. The electrical characteristics of the capacitive electrode depend on various parameters.
Methods: In this work, we proposed a Capacitive Electrode (CE) with optimal Shielding and Guarding design (SGD). The gold was coated over the copper on the base material FR4 is used for electrode material. Three different designs were proposed. The performance of each design was evaluated using the Finite Element Method (FEM).
Results: On comparing all the electrophysical parameters of the three designs, the design: ”The shielding was designed in such a way that it conversed with the electrode, and the guarding was formed as a concentric around the sensing plate” shows optimal SGD, which enables higher performance. The proposed electrode was applied for the ECG application. The optimal electrodes (OE) were placed in the lead I and V1 V2 lead positions. The experimental results conducted by Finite Element Method (FEM) show that the lead I position is the optimal lead position for ECG measurement. The developed Capacitive electrodes were tested to fetch the ECG signal. When comparing the proposed electrode with the traditional 12-lead ECG system and the AD8232 sensor, the SNR value shows better signal quality for the proposed electrode.
Conclusion: This research mainly concentrates on the electrical characteristics of the capacitive electrodes with optimal shielding and guarding design. The proposed capacitive electrode can also used to fetch other bio-signals.
Identifying factors that determine functional status of stroke patients using partial proportional odds model at Felege Hiwot Referral Hospital, Ethiopia
Authors: Berehan Haymanot B. , Berehan Yebelay B. , Kassie Maru Z. , Motbayhon Habitamu W. , Arega Gezachew G. , Awoke Shegaw M.
Published: August 2024

In many clinical trials, patients who are followed up over time may typically measure repeatedly multiple outcomes. Ignoring such repeatedly measured outcome or biomarker and considering cross-sectional analyses can leads to biased estimates. In this context, we presented partial proportional odds model for longitudinal functional status measures among stroke patients at FHRH Bahir Dar, Ethiopia. We considered 400 patients who had stroke under the medical ward outpatient stroke who started treatment from September 2018 to August 2021 by a retrospective cohort study design. Among 400 stroke patients taken from the repository of FHRH, 188(47%) were females. The patients mean age and corresponding 95%CI was (58; 95%CI=55-58) at baseline. Among different candidates of demographic and clinical variables, age, diabetes, blood urea nitrogen, Glasgow coma scale, white blood cell count, and stroke complication were statistically significant predictors. the ordinal longitudinal modeling approach, provides wide-ranging information about the longitudinal pattern of the functional status. Furthermore, the results of the study can be used as a motivation for future scholars. since stroke is one of the most frequent diseases in Ethiopia as well as in the world, it needs more attention and study in a wider study area.
Bioinformatics analysis and population sample validation of ferroptosis related genes in coronary heart disease
Authors: Li LiHeng , Chen CuiHua , Dong QingHua , Xie YuBo
Published: August 2024

The aim of this study was to investigate the association between coronary heart disease (CHD) and ferroptosis, furthermore, to identify the relevant genes and potential diagnostic biomarkers by bioinformatics analysis. CHD was the main cause of cardiovascular diseases, its pathogenesis was complex. As an iron-dependent form of cell death, ferroptosis was believed to play a significant role in various diseases. Studies have shown that genes associated with ferroptosis include PRKAA2, NOX4, GLS2, and G6PD. Further data analysis and meta-analysis revealed that NOX4 gene expression was significantly upregulated in patients with CHD (P<0.05), which may be related to coronary atherosclerosis and myocardial injury. Additionally, the upregulation of NOX4 expression was validated through RT-PCR. In summary, this study revealed the association between CHD and ferroptosis, providing insights into the pathogenesis of CHD. Genes such as NOX4 may serve as diagnostic biomarkers for CHD, offering new perspectives and approaches for its early diagnosis and treatment.
Influence of MMP12 Single Nucleotide Polymorphism rs586701 on the Prognosis of Primary Lung Cancer in Chinese Han Chinese
Authors: Xu Chang , Du Wei , Sun Zhenyu , Gao Zhunyi , Xiao Man , Guo Shicheng , Wang Yi , Wang Haijian , Li Qiang , Wu Junjie , Shen Bo , Shang Yan , Jiang Gengxi , Bao Yang
Published: August 2024

Purpose
Lung cancer is now one of the most common cancers in the world, with a high mortality rate and poor prognosis. Predicting the prognosis of lung cancer patients and using this information to develop treatment strategies and interventions is important for prolonging patient survival. A stratified analysis was conducted on Han Chinese primary lung cancer patients in the Chinese population, with the objective of investigating the relationship between matrix metalloproteinase 12(MMP12) gene polymorphism rs586701 and the prognosis of lung cancer patients.
Methods
A total of 888 Han Chinese primary lung cancer patients (exclusive of minors) were recruited between January and November 2009 (10 months) and provided written informed consent. The study included subjects from Changhai Hospital of the Naval Medical University (Second Military Medical University) and Taizhou Institute of Health Sciences of Fudan University. A total of 49 subjects were excluded due to incomplete data collection for various reasons. Blood samples were collected from 839 patients diagnosed with lung cancer, and genomic DNA was extracted for genotyping using SNPscan technology. In order to adjust for multiple factors, the data was stratified by age, sex, smoking status, family history of malignancy, TNM stage, and lung cancer tissue type. The association between lung cancer prognosis and genotype was then analyzed using a multivariate Cox proportional risk model.
Results
A polymorphism in the MMP12 gene, specifically the T > G variant at position 586701, has been associated with a reduction in survival time in lung cancer patients. In the overall sample, patients with the TG genotype (TG vs TT, adjusted HR = 1.21, 95% CI: 1.01–1.44, P = 0.035) exhibited a shorter survival time and a worse prognosis. Stratified analyses showed that among male lung cancer patients, lung cancer patients younger than 60 years old, and smoking lung cancer patients, patients with the TG genotype had a lower survival time than patients with the TT genotype (adjusted risk ratio HR = 1.24, 95% CI: 1.01–1.52, P = 0.04; HR = 1.58, 95% CI: 1.18–2.12, P = 0.002; HR= (1.30, 95% CI: 1.06–1.61, P = 0.013). In patients with SCC and NSCLC, survival time was shorter in patients with the TG genotype (TG vs TT, adjusted risk ratio HR = 1.48, 95% CI: 1.10–2.01, P = 0.010; HR = 1.21, 95% CI: 1.01–1.46, P = 0.038). In the designed genetic model, the dominant genotype TG + GG was associated with a shorter survival time and worse prognosis among lung cancer patients aged less than 60 years (TG + GG vs TT, adjusted risk ratio HR = 1.43, 95% CI: 1.07–1.90, P = 0.014).
Conclusion
The MMP12 polymorphism rs586701 may be associated with the prognosis of lung cancer. Specifically, the MMP12 polymorphism rs586701 T > G has been found to lead to a worse prognosis.
Florida man exonerated: an amphipod case study on the implications of cryptic species complexes and global invasions.
Authors: Sawicki Thomas R , Cannizzaro A. G.
Published: August 2024

The crangonyctid amphipod Crangonyx floridanus is considered to have a natural distribution across much of the eastern United States and is thought to be invasive in parts of the western United States, Japan, and the British Isles. Recent investigations have revealed highly endemic cryptic species among populations of Crangonyx floridanus sensu lato occurring at approximately 30 oN latitude in the Panhandle of Florida, USA. To date, none of the published cryptic lineages of C. floridanus sensu lato phylogenetically align with the invasive populations. Despite years of collecting efforts, C. floridanus sensu stricto has not been identified from its type locality in Highlands Hammock State Park, in Highlands County, Florida, since its initial collection in 1962. To assess the status of C. floridanus sensu stricto, environmental DNA was used to analyze the V4/V5 hypervariable regions of the nuclear 18S rRNA gene from the type locality. These data revealed that the species has not been extirpated from the type locality, and that it is not the source of the invasive populations. In addition, populations of C. floridanus sensu lato collected for this study from Alabama, Ohio, and Pennsylvania, USA, displayed 100% identity to invasive populations in the western United States, Japan, and the British Isles, at both the 18S rRNA and mitochondrial cytochrome c oxidase subunit I genes. This study demonstrates that traditional diagnostic characteristics may lead to incorrect assumptions about the taxonomic status of invasive species.
Implementation of a Novel TRIZ-Based Model to Increase the Reporting of Adverse Events in the Healthcare Center
Authors: Lee Jiun-Yih , Lee Pei-Shan , Chiang Cheng-Hsien , Chen Yi-Ping , Chen Chiung-Ju , Huang Yuan-Ming , Chiu Jlan-Ren , Yang Pei-Ching , Chang Jui-Ting , Yeh Chen-An
Published: August 2024

Objectives
Underreporting of adverse events in healthcare systems is a global concern. This study aims to address the underreporting of adverse events by implementing a TRIZ-based model to identify and overcome barriers to reporting, thus filling gaps in current reporting practices and improving incident recognition.
Methods
A TRIZ (Theory of Inventive Problem Solving) approach was adopted, integrating with SERVQUAL methodologies to design interventions. Preintervention and postintervention surveys were conducted to evaluate changes in barriers to reporting and the recognition of adverse events. Statistical analyses were performed to assess the effectiveness of the interventions.
Findings:
Barriers to reporting AEs significantly decreased, and recognition improved. Monthly reported cases rose from 33.7 to 50.3 (p = 0.000), demonstrating the effectiveness of the TRIZ-based interventions.
Conclusions
Implementing a TRIZ-based model significantly improved adverse event reporting by overcoming identified barriers and enhancing the recognition of reportable events. Future research should explore the long-term sustainability of these interventions and their broader applicability in diverse healthcare settings.
High concentrations of L-lysine cause mitochondrial damage and necrosis in isolated pancreatic acinar cells
Authors: Végh Eszter T. , Balla Zsolt , Tóth Brigitta , Elperin Jason , Speakman Steven , Pallagi Petra , Rázga Zsolt , Kiss Lóránd , Gukovskaya Anna , Rakonczay Zoltán
Published: August 2024

Intraperitoneal administration of high doses of basic amino acids, such as L-lysine (L-Lys), L-arginine (L-Arg) or L-ornithine (L-Orn) induces acute pancreatitis in rodents. Although the exact mechanism of their action is not fully understood, the role of mitochondria has been implicated. We aimed to investigate the effects of basic amino acids, particularly L-Lys, on isolated pancreatic acinar cells. Isolated mouse or rat pancreatic acinar cells were treated with high concentrations (10–60 mM) of L-Lys, L-Arg or L-Orn. The morphology of acinar mitochondria was observed by electron microscopy. The function of mitochondria was assessed by mitochondrial membrane potential (∆Ψm) and cellular ATP level measurements. Changes in intracellular Ca2+ concentration ([Ca2+]i), trypsin activity and cellular viabilities were also determined. Treatment of acinar cells with L-Lys caused mitochondrial swelling. L-Lys and L-Orn dramatically decreased ∆Ψm after 6 h of treatment, whereas L-Arg reduced ∆Ψm after 3 h of treatment. Intracellular ATP levels were also reduced by basic amino acids. L-Lys did not alter [Ca2+]i and did not induce early trypsinogen activation. Furthermore, L-Lys administration primarily caused acinar necrosis. Overall, L-Lys primarily damaged pancreatic acinar mitochondria and caused necrotic cell death without affecting [Ca2+]i.
Technology for obtaining linseed protein concentrate minimized in antinutritional factors
Authors: Cargnin Gregorio , Adorian Taida Juliana , Falcone Diuly Bortoluzzi , de Freitas Ana Maria Liberalesso , Robalo Silvino Sasso , Silva Stéfane Sauzem , Silva Leila Picolli da
Published: August 2024

The linseed processing was carried out on a laboratory scale to obtain the linseed protein concentrate (LPC), using the physical method. The analyzes were carried out on whole grains (WG), demucilated grains (GD) and LPC. In the fractions obtained, the chemical composition (dry matter, ash, lipids, crude protein, total dietary fiber, soluble fiber and insoluble fiber), total phenolic compounds and physicochemical properties were evaluated in the obtained fractions. The amino acid profile was determined for WG, GD and LPC. Protein molecular weight electrophoresis was determined for WG, GD and LPC. The results indicated that, in the physical process for protein concentration, LPC presented a protein content 96% higher than that of linseed grains and obtained less than 8% of lipids in its composition. Furthermore, LPC improved the amino acid profile and at the phenolic compounds of the different fractions of flaxseed, we see that their contents were concentrated in the LPC, which was 83.75% higher than the WG. The electrophoresis results demonstrate that physical processing caused a reduction in the molecular weight of the reducing and non-reducing flaxseed proteins. The present study allows us to conclude that it is possible to separate flaxseed into different fractions, using physical processes, obtaining a protective concentrate. The inclusion of these products in animal feed needs to be evaluated to determine levels of acceptance.
The effect of brolucizumab on diabetic macular edema and ischemia; a real world analysis
Authors: Chronopoulos Argyrios , Sas Lea Karla , Holzwarth Jakob Wilhelm , Hattenbach Lars-Olof
Published: August 2024

Objective The response to intravitreal brolucizumab on diabetic macular edema and macular ischemia was studied in treatment-refractory and treatment-naïve patients.Methods Patients with either treatment resistant or treatment naive diabetic macular edema were studied retrospectively before and after intravitreal brolucizumab. All patients underwent a comprehensive ophthalmological examination including optical coherence tomography (OCT) and OCT-angiography (OCT-A) to compare their macular edema and macular ischemia before initiation of intravitreal brolucizumab treatment and at the end of the upload phase.Results A total of 31 eyes of 27 diabetic patients were examined. 19 eyes were switched to brolucizumab after long prior anti-VEGF treatment history; 12 eyes were treatment naive with respect to macular edema. All eyes responded positively to brolucizumab with complete resolution of diabetic macular edema: group 1: 369 vs 302 µm (p = 0.007), group 2: 415 vs 259 µm (p = 0.01). All eyes also manifested improvement in vision: group 1: 0.3 vs 0 .6 (0.5 ± 0.5 logMAR vs. 0.22 ± 0.5 logMAR) (p = 0.01), group 2: 0.3 vs 0.5 (0.5 ± 0.7 logMAR vs. 0.2 ± 0.7 logMAR) (p = 0.00002). Interestingly, in group 2 there was also significant improvement in vessel and perfusion density, especially in the central and inner diameter: central vessel density of 6.55 vs 9.03 mm/mm2 (p = 0.008), inner of 11.2 vs 13, 9 mm/mm2 (p = 0.02), and central perfusion density of 15.5% vs 20.7% (p = 0.02). Although not statistically significant, there was also a tendency to improved foveal circularity.Conclusion Intravitreal brolucizumab leads to significant functional and anatomical improvement in diabetic macular edema and has also a beneficial effect on macular ischemia reflected by foveal capillary anatomy and perfusion density.
Clinical characteristics of acute acquired concomitant esotropia in China: a retrospective study
Authors: Liang Shuting , Wu Xi , Wang Lejin
Published: August 2024

Purpose
To compare the clinical characteristics of acute acquired concomitant esotropia (AACE) patients in different age of onset groups before and after the coronavirus disease 2019 (COVID-19) pandemic.
Methods
A retrospective study included 126 patients who underwent AACE surgery from January 2015, and December 2023. Detailed information on demographic data and clinical characteristics was collected and compared between different age of onset groups.
Results
A total of 115 patients (91.3%) spent more than four hours a day for near work. The near and distance deviations were 20.00 (12.00, 30.00) prism diopters (PD) and 25.00 (20.00, 35.00) PD, respectively, which has significant difference (P < 0.001). The mean spherical equivalent (SE) of non-dominant and dominant eye were − 4.00 (-5.31, -2.50) diopters (D) and − 4.00 (-5.31, -2.25) D, respectively (P = 0.001). The child group included 40 patients, while the adult group included 86 patients. The near and distance deviations in the child group were 25.00 (16.50, 70.00) PD and 35.00 (25.00, 71.50) PD, respectively, and those in the adult group were 20.00 (12.00, 30.00) PD and 25.00 (18.00, 35.00) PD, respectively. The near and distance deviation were both significantly larger in the child group compared with the adult group (P = 0.02 and P = 0.004), while no statistically significant difference was observed in duration of disease (P = 0.128). The mean SE of dominant eye and non-dominant eye both had larger degrees of myopia in the adult group than the child group (P = 0.001 and P = 0.028). The duration of disease in the pre-pandemic group was significantly longer than the post-pandemic group (P < 0.001).
Conclusions
The non-dominant eye had a larger degree of myopia than the dominant eye, and the distance esodeviation was larger than the near esodeviation in AACE patients. Children presented a larger esodeviation and were less myopic than that of adults. The disease progressed more quickly to require surgery and the number of AACE surgeries increased remarkably after the COVID-19 pandemic.
Evaluation of the effect of a pneumatic dental elevator on tooth extraction
Authors: Feng Xiancheng , Tian Yiyuan , Du Qingjie , Wang Keyi , Chen Ruiqi , Du Juanjuan , Dai Pengxiu , Zhang Xinke
Published: August 2024

Pneumatic tooth elevators are newly developed surgical instruments that utilize compressed air to drive a small, oscillating tip that can gently loosen the tooth from its socket. However, the effect of pneumatic tooth elevators in veterinary clinical practice is not yet clear. In this study, 6 dogs were used as experimental animals. and maxillary first premolars and mandibular first molars were extracted using traditional (control group) and pneumatic dental elevators (experimental group). Physiological parameters, extraction time, intraoperative complications, hematological examination, and tissue healing were compared to investigate the effect of pneumatic dental elevators. The results showed that compared to the control group, the experimental group had a smaller increase in heart rate and respiratory rate, and the time required to extract the mandibular first molar was less. The incidence of complications in the control group was 25% (3/12), while there were no complications in the experimental group. Additionally, all cases in the control group had defects in the mandibular alveolar bone, while only 2/3 of the cases in the experimental group had defects, and the degree of defect was less than that of the control group. The white blood cell count and C-reactive protein (CRP) concentration in the experimental group were significantly lower than those in the control group. The early wound healing score (EHS), soft tissue healing index, and trabecular area in the experimental group were all significantly greater than those in the control group. The above results indicate that using pneumatic dental elevators reduces the degree of pain during the surgical process, takes less time, causes less damage to periodontal tissues, reduces the severity of postoperative inflammation, and promotes better tissue healing. This study provides a theoretical basis and technical reference for the clinical application of the new type of pneumatic dental elevator, promoting its wider application.