bluuebunny/crossref_metadata_2025 huggingface dataset:
New Herbal Biomedicines for the Topical Treatment of Dermatological Disorders
Authors: Hoffmann Julia , Gendrisch Fabian , Schempp Christoph Mathis , Wölfle Ute
Published: February 2020

Herbal extracts and isolated plant compounds play an increasing role in the treatment of skin disorders and wounds. Several new herbal drugs, medicinal products and cosmetic products for the treatment of various skin conditions have been developed in recent years. In this nonsystematic review, we focus on herbal drugs that were tested in controlled clinical studies or in scientifically sound preclinical studies. The herbal biomedicines are intended to treat atopic dermatitis (St. John’s wort, licorice, tormentil, bitter substances, evening primrose), psoriasis (araroba tree, lace flower, barberry bark, indigo, turmeric, olibanum, St. John’s wort), actinic keratosis (birch bark, petty spurge), herpes simplex (lemon balm, sage and rhubarb), rosacea (green tea, licorice, tormentil) and acne vulgaris (tea tree oil, green tea, hop), or to improve photo protection (green tea, Dyer’s weed, cocoa tree, carotinoids, licorice), aesthetic dermatology (licorice, pine bark, gotu kola) and wound healing (birch bark, onion).
Effects of Anchomanes difformis on Inflammation, Apoptosis, and Organ Toxicity in STZ-Induced Diabetic Cardiomyopathy
Authors: Alabi Toyin D. , Chegou Novel N. , Brooks Nicole L. , Oguntibeju Oluwafemi O.
Published: February 2020

Persistent hyperglycemia is known to cause enhanced generation of reactive oxygen species in diabetes. Several inflammatory cytokines are induced by oxidative stress, and their release also leads to increased oxidative stress; this makes oxidative stress one of the important factors in the development of chronic inflammation and other immune responses. These have been implicated in the development of diabetic complications such as nephropathy and cardiomyopathy. Anchomanes difformis has been shown to possess antioxidant and anti-inflammatory potentials. The present study investigated the immunomodulatory potential and the antiapoptotic ability of Anchomanes difformis to ameliorate heart toxicity and injury in type II diabetes. Two weeks of fructose (10%) administration followed by single intraperitoneal injection of streptozotocin (40 mg/kg) were used to induce type II diabetes in male Wistar rats. Leaf extract (aqueous) of Anchomanes difformis (200 and 400 mg/kg) was administered orally for six weeks. Blood glucose concentrations and body weights before and after interventions were determined. Interleukin (IL)-1β, IL-6, IL-10, IL-18, monocyte chemoattractant protein 1 (MCP-1), and tumor necrosis factor alpha (TNFα) were measured in the heart homogenates. Catalase (CAT), superoxide dismutase (SOD), total protein, oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive substances (TBARS), and heart-type fatty acid-binding protein (H-FABP) levels were determined. Expressions of transcription factors (Nrf 2 and NFkB/p65) and apoptotic markers were also investigated in the heart. Anchomanes difformis administration reduced pro-inflammatory cytokines, increased anti-inflammatory markers, and enhanced antioxidant defense in the heart of diabetic treated animals. Anchomanes difformis is a new, promising therapeutic agent that can be explored for the treatment of pathological conditions associated with immune responses and will be a useful tool in the management of associated diabetic complications.
Immunomodulatory Roles of PARP-1 and PARP-2: Impact on PARP-Centered Cancer Therapies
Authors: Yélamos José , Moreno-Lama Lucia , Jimeno Jaime , Ali Syed O.
Published: February 2020

Poly(ADP-ribose) polymerase-1 (PARP-1) and PARP-2 are enzymes which post-translationally modify proteins through poly(ADP-ribosyl)ation (PARylation)—the transfer of ADP-ribose chains onto amino acid residues—with a resultant modulation of protein function. Many targets of PARP-1/2-dependent PARylation are involved in the DNA damage response and hence, the loss of these proteins disrupts a wide range of biological processes, from DNA repair and epigenetics to telomere and centromere regulation. The central role of these PARPs in DNA metabolism in cancer cells has led to the development of PARP inhibitors as new cancer therapeutics, both as adjuvant treatment potentiating chemo-, radio-, and immuno-therapies and as monotherapy exploiting cancer-specific defects in DNA repair. However, a cancer is not just made up of cancer cells and the tumor microenvironment also includes multiple other cell types, particularly stromal and immune cells. Interactions between these cells—cancerous and non-cancerous—are known to either favor or limit tumorigenesis. In recent years, an important role of PARP-1 and PARP-2 has been demonstrated in different aspects of the immune response, modulating both the innate and adaptive immune system. It is now emerging that PARP-1 and PARP-2 may not only impact cancer cell biology, but also modulate the anti-tumor immune response. Understanding the immunomodulatory roles of PARP-1 and PARP-2 may provide invaluable clues to the rational development of more selective PARP-centered therapies which target both the cancer and its microenvironment.
PARP Inhibitors as Therapeutics: Beyond Modulation of PARylation
Authors: Min Ahrum , Im Seock-Ah
Published: February 2020

Poly (ADP-ribose) polymerase (PARP) 1 is an essential molecule in DNA damage response by sensing DNA damage and docking DNA repair proteins on the damaged DNA site through a type of posttranslational modification, poly (ADP-Ribosyl)ation (PARylation). PARP inhibitors, which inhibit PARylation through competitively binding to NAD+ binding site of PARP1 and PARP2, have improved clinical benefits for BRCA mutated tumors, leading to their accelerated clinical application. However, the antitumor activities of PARP inhibitors in clinical development are different, due to PARP trapping activity beyond blocking PARylation reactions. In this review, we comprehensively address the current state of knowledge regarding the mechanisms of action of PARP inhibitors. We will also discuss the different effects of PARP inhibitors in combination with cytotoxic chemotherapeutic agents regarding the mechanism of regulating PARylation.
Water Balance and Soil Moisture Deficit of Different Vegetation Units under Semiarid Conditions in the Andes of Southern Ecuador
Authors: Fries Andreas , Silva Karen , Pucha-Cofrep Franz , Oñate-Valdivieso Fernando , Ochoa-Cueva Pablo
Published: February 2020

Water availability in semiarid regions is endangered, which is not only due to changing climate conditions, but also to anthropogenic land use changes. The present study analyzed the annual and monthly water balance (WBc) and the soil moisture deficit (Ds) for different vegetation units under semiarid conditions in the Andes of southern Ecuador, based on limited meteorological station data and field measurements (soil samples). To calculate crop evapotranspiration (ETc) the Blaney–Criddle method was applied, and the specific crop factor (Kc) included, because only temperature (T) and precipitation (P) data were available. By means of the soil samples the water retention capacity (RC) of the different soil types present in the study area were estimated, which, in combination with WBc, provided reliable results respective to water surpluses or deficits for the different vegetation units. The results indicated highest Ds for cultivated areas, particularly for corn and sugarcane plantations, where annual deficits up to −1377.5 mm ha−1 and monthly deficits up to −181.1 mm ha−1 were calculated. Natural vegetation cover (scrubland, forest and paramo), especially at higher elevations, did not show any deficit throughout the year (annual surpluses up to 1279.6 mm ha−1; monthly surpluses up to 280.1 mm ha−1). Hence, it could be concluded that the prevailing climate conditions in semiarid regions cannot provide the necessary water for agricultural practices, for which reason irrigation is required. The necessary water can be supplied by areas coved by natural vegetation, but these areas are endangered due to population growth and the associated land use changes.
Efficiency of Novel Antimicrobial Coating Based on Iron Nanoparticles for Dairy Products’ Packaging
Authors: Ligaj Marta , Tichoniuk Mariusz , Cierpiszewski Ryszard , Foltynowicz Zenon
Published: February 2020

The main function of food packaging is to maintain food’s quality and safety. The use of active packaging, including antimicrobial materials, can significantly extend the shelf life of food. Many of these packaging solutions are based on the application of polymer films containing metal nanoparticles (e.g., Ag, Au, Cu) or metal oxides (e.g., TiO2, ZnO, MgO). However, the use of iron nanoparticles is rarely mentioned. In the study, polylactide (PLA) films containing zero-valent iron (ZVI) were made by casting method. Pure PLA films and PLA films with the addition of Fe2O3 were used as comparative materials. The composition and structure of ZVI/PLA films were evaluated with scanning electron microscopy. The XRD spectra performed on ZVI/PLA films confirmed the presence of iron in the packaging material and revealed their oxide form (Fe2O3). The addition of zero-valent iron in the concentration 1%, 3%, or 5% resulted in the formation of crystallographic planes measuring 40.8, 33.6, and 28.6 nm, respectively. The color and gloss of the films, and their antimicrobial activity against bacteria (Bacillus subtilis, Escherichia coli, Staphylococcus epidermidis) and fungi (Geotrichum candidum, Rhodotorula rubra) were also examined. The PLA films with addition of 3% of ZVI (w/w) inhibited the growth of all tested organisms in contrast to PLA and PLA/Fe2O3 films. The addition of ZVI to polymer matrix caused changes in its appearance and optical properties. The ZVI/PLA coating used on polyolefin film allowed to extend the shelf life of goat cheese packed in examined material to 6 weeks. Considering the antimicrobial properties of the ZVI/PLA films and PLA biodegradability the obtained material can be successfully applied in the food industry.
Co-Evolution of Complex Network Public Goods Game under the Edges Rules
Authors: Sun Xingping , Li Yibing , Kang Hongwei , Shen Yong , Peng Jian , Wang Haoyu , Chen Qingyi
Published: February 2020

The reconnection of broken edges is an effective way to avoid drawback for the commons in past studies. Inspired by this, we proposed a public goods game model under the edges rules, where we evaluate the weight of edges by their nodes’ payoff. The results proved that the game obtains a larger range of cooperation with a small gain factor by this proposed model by consulting Monte Carlo simulations (MCS) and real experiments. Furthermore, as the following the course of game and discussing the reason of cooperation, in the research, we found that the distribution entropy of the excess average degree is able to embody and predict the presence of cooperation.
Material Identification Using a Microwave Sensor Array and Machine Learning
Authors: Harrsion Luke , Ravan Maryam , Tandel Dhara , Zhang Kunyi , Patel Tanvi , K. Amineh Reza
Published: February 2020

In this paper, a novel methodology is proposed for material identification. It is based on the use of a microwave sensor array with the elements of the array resonating at various frequencies within a wide range and applying machine learning algorithms on the collected data. Unlike the previous microwave sensing systems which are mainly based on a single resonating sensor, the proposed methodology allows for material characterization over a wide frequency range which, in turn, improves the accuracy of the material identification procedure. The performance of the proposed methodology is tested via the use of easily available materials such as woods, cardboards, and plastics. However, the proposed methodology can be extended to other applications such as industrial liquid identification and composite material identification, among others.
Driver’s Social Relationship Based Clustering and Transmission in Vehicle Ad Hoc Networks (VANETs)
Authors: Li Lin , Wang Wenjian , Gao Zhenhai
Published: February 2020

Clustering is a technique for dividing a network into different group of nodes and managing the transmission of data among the interacting nodes, to improve the effectiveness and safety of information transfer. Clustering have been well studied and applied in traditional mobile networks. However, vehicle networks have short connection time, frequently changing topology, and other unique properties that conventional clustering cannot transfer well. The vehicle nodes in Vehicle Ad-hoc Networks (VANETs) are most directly affected by the surrounding vehicle nodes and exchanged information with them. However, this will cause network congestion or even the spread of malicious messages. The inclusion of vehicle’s (driver’s) social relationships in vehicle communication clustering will increase the degree of trust between vehicle nodes, making communication more purposeful and accurate. This study proposed a new clustering for vehicle networks that is based on drivers’ social relationship combined with the instantaneous position and speed of the vehicle node. Simulation results showed that this clustering method can improve the effectiveness of information transmission and increase the utilization of the application layer.
A Novel Control Scheme for Multi-Terminal Low-Frequency AC Electrical Energy Transmission Systems Using Modular Multilevel Matrix Converters and Virtual Synchronous Generator Concept
Authors: Al-Tameemi Mustafa , Miura Yushi , Liu Jia , Bevrani Hassan , Ise Toshifumi
Published: February 2020

This paper proposes a new control scheme for the low frequency AC transmission (LFAC) system aiming at extending the point-to-point configuration to form a multi-terminal electrical energy network. The multi-terminal low frequency ac (MT-LFAC) system configuration is based on the use of modular multilevel matrix converters (M3Cs) and virtual synchronous generator (VSG) control. The M3C is the next ac/ac converter generation, which is used as an interface with the conventional AC network and the LFAC electrical energy system. Application of VSG control is proposed to enable proper power sharing, to provide synchronization of each terminal, and frequency stabilization, thus, to offer multiterminal forming capability. Two different operation modes are applied in the system to damp the frequency deviation after a dynamic perturbation, which provides additional stabilization feature to the VSG. Frequency restoration mode and commanded mode of power sharing are applied as dynamic states to validate the robustness of the VSG control system. Besides, to solve the negative impact of low X/R ratio in the LFAC electrical energy system, we enhance the VSG control by proposing a virtual-impedance-based solution, which increases the output total impedance on the low frequency side and prevents the coupling between P and Q. The operation of the proposed system is examined by simulation results with a precise model of M3Cs in the PSCAD/ EMTDC software environment (version 4.2.1, Winnipeg, MB, Canada).
Investigation of Start-Up Characteristics of Thermosyphons Modified with Different Hydrophilic and Hydrophobic Inner Surfaces
Authors: Ma Xiaolong , Zhao Zhongchao , Jiang Pengpeng , Yang Shan , Li Shilin , Chen Xudong
Published: February 2020

In this paper, the influence of wettability properties on the start-up characteristics of two-phase closed thermosyphons (TPCTs) is investigated. Chemical coating and etching techniques are performed to prepare the surfaces with different wettabilities that is quantified in the form of the contact angle (CA). The 12 TPCTs are processed including the same CA and a different CA combination on the inner surfaces inside both the evaporator and the condenser sections. For TPCTs with the same wettability properties, the introduction of hydrophilic properties inside the evaporator section not only significantly reduces the start-up time but also decreases the start-up temperature. For example, the start-up time of a TPCT with CA = 28° at 40 W, 60 W and 80 W is 46%, 50% and 55% shorter than that of a TPCT with a smooth surface and the wall superheat degrees is 55%, 39% and 28% lower, respectively. For TPCTs with combined hydrophilic and hydrophobic properties, the start-up time spent on the evaporator section with hydrophilic properties is shorter than that of the hydrophobic evaporator section and the smaller CA on the condenser section shows better results. The start-up time of a TPCT with CA = 28° on the evaporator section and CA = 105° on the condenser section has the best start-up process at 40 W, 60 W and 80 W which is 14%, 22% and 26% shorter than that of a TPCT with smooth surface. Thus, the hydrophilic and hydrophobic modifications play a significant role in promoting the start-up process of a TPCT.
In Situ Acoustic Treatment of Anaerobic Digesters to Improve Biogas Yields
Authors: Loughrin John , Antle Stacy , Sistani Karamat , Lovanh Nanh
Published: February 2020

Sound has the potential to increase biogas yields and enhance wastewater degradation in anaerobic digesters. To assess this potential, two pilot-scale digestion systems were operated, with one exposed to sound at less than 10 kHz and with one acting as a control. Sounds used were sine waves, broadband noise, and orchestral compositions. Weekly biogas production from sound-treated digesters was 18,900 L, more than twice that of the control digester. The sound-treated digesters were primarily exposed to orchestral compositions, because this made cavitational events easier to identify and because harmonic and amplitude shifts in music seem to induce more cavitation. Background recordings from the sound-treated digester were louder and had more cavitational events than those of the control digester, which we ascribe to enhanced microbial growth and the resulting accelerated sludge breakdown. Acoustic cavitation, vibrational energy imparted to wastewater and sludge, and mixing due to a release of bubbles from the sludge may all act in concert to accelerate wastewater degradation and boost biogas production.
Bio-Functional and Structural Properties of Pasta Enriched with a Debranning Fraction from Purple Wheat
Authors: Abbasi Parizad Parisa , Marengo Mauro , Bonomi Francesco , Scarafoni Alessio , Cecchini Cristina , Pagani Maria Ambrogina , Marti Alessandra , Iametti Stefania
Published: February 2020

A colored and fiber-rich fraction from the debranning of purple wheat was incorporated at 25% into semolina- and flour-based pasta produced on a pilot-plant scale, with the aim of increasing anthocyanin and total phenolic content with respect to pasta obtained from whole pigmented grains. The debranning fraction impaired the formation of disulfide-stabilized protein networks in semolina-based systems. Recovery of phenolics was impaired by the pasta making process, and cooking decreased the phenolic content in both enriched samples. Cooking-related losses in anthocyanins and total phenolics were similar, but anthocyanins in the cooked semolina-based pasta were around 20% of what was expected from the formulation. HPLC (High Performance Liquid Chromatography) profiling of phenolics was carried out on extracts from either type of enriched pasta both before and after cooking and indicate possible preferential retention of specific compounds in each type of enriched pasta. Extracts from cooked samples of either enriched pasta were tested as inhibitors of enzymes involved in glucose metabolism and uptake, as well as for their capacity of suppressing the response to inflammatory stimuli. Results of both biological tests indicate that the phenolics in extracts from both cooked pasta samples had inhibitory capacities higher than extracts of the original debranning fraction at identical concentrations of total bioactives.
Massive Star Formation in the Ultraviolet Observed with the Hubble Space Telescope
Authors: Leitherer Claus
Published: February 2020

Spectroscopic observations of a massive star formation in the ultraviolet and their interpretation are reviewed. After a brief historical retrospective, two well-studied resolved star clusters and the surrounding H II regions are introduced: NGC 2070 in the Large Magellanic Cloud and NGC 604 in M33. These regions serve as a training set for studies of more distant clusters, which can no longer be resolved into individual stars. Observations of recently formed star clusters and extended regions in star-forming galaxies in the nearby universe beyond the Local Group are presented. Their interpretation relies on spectral synthesis models. The successes and failures of such models are discussed, and future directions are highlighted. I present a case study of the extraordinary star cluster and giant H II region in the blue compact galaxy II Zw 40. The review concludes with a preview of two upcoming Hubble Space Telescope programs: ULLYSES, a survey of massive stars in nearby galaxies, and CLASSY, a study of massive star clusters in star-forming galaxies.
Adolescent Profiles According to Their Beliefs and Affinity to Sexting. A Cluster Study
Authors: Soriano-Ayala Encarnación , C. Cala Verónica , Dalouh Rachida
Published: February 2020

Sexting consists of sending, receiving, and distributing images of sexually suggestive content through electronic devices. This practice is one of the new ways of linking sex affectively through virtual environments, especially in adolescence. However, not all young people have the same relationship with the practice of sexting. This study of a sample of 603 Spanish and Moroccan adolescents residing in Andalusia analyzes beliefs towards sexting as part of a virtual sexuality and the perception of those who carry it out, defining profiles of affinity to sexting. The cluster analysis reveals the existence of three predominant profiles: adolescents who show a sexting-philia, perceiving it as a fun, flirty, and daring practice; sexting-phobes, who consider sexting to be characteristic of people, or attitudes, who are desperate, impolite, and conflicting; and a third ambivalent profile of people who appreciate the practice as something fun but conflicting. The majority discourse is one that presents a positive view of this phenomenon. Young people also recognize that sexting has some characteristics of virtual sexuality, such as a loss of privacy and a distance between virtual and real behavior. These findings allow us to deepen our understanding of the new practices of relationships and offer measures for the prevention of the associated risks.
Application of the Anammox in China—A Review
Authors: Wen Ruolan , Jin Yue , Zhang Wenjie
Published: February 2020

Anaerobic ammonia oxidation (anammox) has been one of the most innovative discoveries for the treatment of wastewater with high ammonia nitrogen concentrations. The process has significant advantages for energy saving and sludge reduction, also capital costs and greenhouse gases emissions are reduced. Recently, the use of anammox has rapidly become mainstream in China. This study reviews the engineering applications of the anammox process in China, including various anammox-based technologies, selection of anammox reactors and attempts to apply them to different wastewater treatment plants. This review discusses the control and implementation of stable reactor operation and analyzes challenges facing mainstream anammox applications. Finally, a unique and novel perspective on the development and application of anammox in China is presented.
Meta-Analysis on the Effects of Transcranial Direct Current Stimulation on Naming of Elderly with Primary Progressive Aphasia
Authors: Byeon Haewon
Published: February 2020

Purpose: This study aimed to conduct a qualitative evaluation by synthesizing previous studies on the effect of transcranial direct current stimulation (tDCS) on primary progressive aphasia (PPA)’s naming ability and prove the effects of tDCS mediation on PPA naming using meta-analysis. Methods: This study searched literature published from January 2000 to July 2019 using four academic databases (i.e., PubMed, Web of Science, MEDLINE, and Cochrane Library). The final seven publications were systematically evaluated and meta-analysis was conducted for two papers. The effect size was estimated by a standard mean difference (SMD) using Hedge’s g, and the significance of effect size was confirmed using the 95% confidence interval. Results: The results of seven previous studies’ quality assessments ranged from 15 to 26, which were rated above adequate. The results of the meta-analysis showed that the effect size was 0.82 (95% CI: 0.16–1.47), which was a significant ‘large effect’. Conclusions: This meta-analysis proved that tDCS intervention significantly improved the naming performance of PPA. Future studies must confirm the effects of tDCS on naming intervention by using meta-analysis including many RCT studies.
Parent and Clinician Perspectives on the Participation of Children with Cerebral Palsy in Community-Based Football: A Qualitative Exploration in a Regional Setting
Authors: Sivaratnam Carmel , Howells Katherine , Stefanac Nicole , Reynolds Kelly , Rinehart Nicole
Published: February 2020

The current study aimed to qualitatively explore parent and clinician perspectives on the factors influencing participation in a community-based Australian-Rules Football program for five to 12-year-old children with cerebral palsy (CP) in a regional setting. Six allied-health clinicians and two parents of children with CP participated in focus groups exploring factors influencing participation. Thematic analysis indicated seven key factors influencing participation—of which, six were environmental factors and one was related to child characteristics. Environmental factors included resources, communication, knowledge and previous experience, attitudes and expectations, game factors and community relevance. Child characteristics included age, preferences, confidence, as well and cognitive and physical functioning. Notwithstanding limitations, the current study highlights the central role of ‘people factors’ in the child’s environment, in facilitating participation in community-based physical activity.
Design of a Network Optimization Platform for the Multivehicle Transportation of Hazardous Materials
Authors: Dong Sheng , Zhou Jibiao , Ma Changxi
Published: February 2020

With economic development, the volume of hazardous materials is increasing, and the potential risks to human beings and the natural environment are expanding. Road transportation has become the main mode of transportation for hazardous materials. Because of the specific characteristics of hazardous materials, if an accident occurs in the transportation process, it often causes mass casualties, serious property and socioeconomic damage, and damage to the ecological environment. Hence, transportation is an important part of the life cycle of hazardous materials. This paper designs an optimization platform for multidestination, multiterminal, and multivehicle networks that transport hazardous materials. The logistics module in TransCAD software is used to construct this platform. By identifying the effective transportation routes considering the transportation risk, sensitive target population, and transportation time of each road section, the entropy method can be used to fuse and obtain the comprehensive impedance value of each road section. Finally, the optimal transportation network of hazardous materials was obtained by the transportation network optimization algorithm in TransCAD. The platform can display the optimal transport program with data windows, text, and maps. The research results provide a reference for relevant departments to scientifically manage the transport of hazardous materials.
Interaction of Aβ42 with Membranes Triggers the Self-Assembly into Oligomers
Authors: Banerjee Siddhartha , Hashemi Mohtadin , Zagorski Karen , Lyubchenko Yuri L.
Published: February 2020

The self-assembly of amyloid β (Aβ) proteins into oligomers is the major pathogenic event leading to Alzheimer’s disease (AD). Typical in vitro experiments require high protein concentrations, whereas the physiological concentration of Aβ is in the picomolar to low nanomolar range. This complicates the translation of results obtained in vitro to understanding the aggregation process in vivo. Here, we demonstrate that Aβ42 self-assembles into aggregates on membrane bilayers at low nanomolar concentrations - a pathway in which the membrane plays the role of a catalyst. Additionally, physiological ionic conditions (150 mM NaCl) significantly enhance on-membrane aggregation, leading to the rapid formation of oligomers. The self-assembly process is reversible, so assembled aggregates can dissociate from the membrane surface into the bulk solution to further participate in the aggregation process. Molecular dynamics simulations demonstrate that the transient membrane-Aβ interaction dramatically changes the protein conformation, facilitating the assembly of dimers. The results indicate peptide–membrane interaction is the critical step towards oligomer formation at physiologically low protein concentrations.
ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways
Authors: Ilyasov Igor R. , Beloborodov Vladimir L. , Selivanova Irina A. , Terekhov Roman P.
Published: February 2020

The 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radical cation-based assays are among the most abundant antioxidant capacity assays, together with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-based assays according to the Scopus citation rates. The main objective of this review was to elucidate the reaction pathways that underlie the ABTS/potassium persulfate decolorization assay of antioxidant capacity. Comparative analysis of the literature data showed that there are two principal reaction pathways. Some antioxidants, at least of phenolic nature, can form coupling adducts with ABTS•+, whereas others can undergo oxidation without coupling, thus the coupling is a specific reaction for certain antioxidants. These coupling adducts can undergo further oxidative degradation, leading to hydrazindyilidene-like and/or imine-like adducts with 3-ethyl-2-oxo-1,3-benzothiazoline-6-sulfonate and 3-ethyl-2-imino-1,3-benzothiazoline-6-sulfonate as marker compounds, respectively. The extent to which the coupling reaction contributes to the total antioxidant capacity, as well as the specificity and relevance of oxidation products, requires further in-depth elucidation. Undoubtedly, there are questions as to the overall application of this assay and this review adds to them, as specific reactions such as coupling might bias a comparison between antioxidants. Nevertheless, ABTS-based assays can still be recommended with certain reservations, particularly for tracking changes in the same antioxidant system during storage and processing.
Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams
Authors: Gögele Clemens , Hahn Judith , Elschner Cindy , Breier Annette , Schröpfer Michaela , Prade Ina , Meyer Michael , Schulze-Tanzil Gundula
Published: February 2020

Reconstruction of ruptured anterior cruciate ligaments (ACLs) is limited by the availability and donor site morbidity of autografts. Hence, a tissue engineered graft could present an alternative in the future. This study was undertaken to determine the performance of lapine (L) ACL-derived fibroblasts on embroidered poly(l-lactide-co-ε-caprolactone) (P(LA-CL)) and polylactic acid (PLA) scaffolds in regard to a tissue engineering approach for ACL reconstruction. Surface modifications of P(LA-CL)/PLA by gas-phase fluorination and cross-linking of a collagen foam using either ethylcarbodiimide (EDC) or hexamethylene diisocyanate (HMDI) were tested regarding their influence on cell adhesion, growth and gene expression. The experiments were performed using embroidered P(LA-CL)/PLA scaffolds that were seeded dynamically or statically with LACL-derived fibroblasts. Scaffold cytocompatibility, cell survival, numbers, metabolic activity, ultrastructure and sulfated glycosaminoglycan (sGAG) synthesis were evaluated. Quantitative real-time polymerase chain reaction (QPCR) revealed gene expression of collagen type I (COL1A1), decorin (DCN), tenascin C (TNC), Mohawk (MKX) and tenomodulin (TNMD). All tested scaffolds were highly cytocompatible. A significantly higher cellularity and larger scaffold surface areas colonized by cells were detected in HMDI cross-linked and fluorinated scaffolds compared to those cross-linked with EDC or without any functionalization. By contrast, sGAG synthesis was higher in controls. Despite the fact that the significance level was not reached, gene expressions of ligament extracellular matrix components and differentiation markers were generally higher in fluorinated scaffolds with cross-linked collagen foams. LACL-derived fibroblasts maintained their differentiated phenotype on fluorinated scaffolds supplemented with a HMDI cross-linked collagen foam, making them a promising tool for ACL tissue engineering.
Engineered Glucose Oxidase Capable of Quasi-Direct Electron Transfer after a Quick-and-Easy Modification with a Mediator
Authors: Suzuki Nanami , Lee Jinhee , Loew Noya , Takahashi-Inose Yuka , Okuda-Shimazaki Junko , Kojima Katsuhiro , Mori Kazushige , Tsugawa Wakako , Sode Koji
Published: February 2020

Glucose oxidase (GOx) has been widely utilized for monitoring glycemic levels due to its availability, high activity, and specificity toward glucose. Among the three generations of electrochemical glucose sensor principles, direct electron transfer (DET)-based third-generation sensors are considered the ideal principle since the measurements can be carried out in the absence of a free redox mediator in the solution without the impact of oxygen and at a low enough potential for amperometric measurement to avoid the effect of electrochemically active interferences. However, natural GOx is not capable of DET. Therefore, a simple and rapid strategy to create DET-capable GOx is desired. In this study, we designed engineered GOx, which was made readily available for single-step modification with a redox mediator (phenazine ethosulfate, PES) on its surface via a lysine residue rationally introduced into the enzyme. Thus, PES-modified engineered GOx showed a quasi-DET response upon the addition of glucose. This strategy and the obtained results will contribute to the further development of quasi-DET GOx-based glucose monitoring dedicated to precise and accurate glycemic control for diabetic patient care.
Guanylyl Cyclase-cGMP Signaling Pathway in Melanocytes: Differential Effects of Altered Gravity in Non-Metastatic and Metastatic Cells
Authors: Ivanova Krassimira , Hemmersbach Ruth
Published: February 2020

Human epidermal melanocytes as melanin producing skin cells represent a crucial barrier against UV-radiation and oxidative stress. It was shown that the intracellular signaling molecule cyclic guanosine-3′,5′-monophosphate (cGMP), generated by the guanylyl cyclases (GCs), e.g., the nitric oxide (NO)-sensitive soluble GC (sGC) and the natriuretic peptide-activated particulate GC (GC-A/GC-B), plays a role in the melanocyte response to environmental stress. Importantly, cGMP is involved in NO-induced perturbation of melanocyte–extracellular matrix interactions and in addition, increased NO production during inflammation may lead to loss of melanocytes and support melanoma metastasis. Further, the NO-sensitive sGC is expressed predominantly in human melanocytes and non-metastatic melanoma cells, whereas absence of functional sGC but up-regulated expression of GC-A/GC-B and inducible NO synthase (iNOS) are detected in metastatic cells. Thus, suppression of sGC expression as well as up-regulated expression of GC-A/GC-B/iNOS appears to correlate with tumor aggressiveness. As the cGMP pathway plays important roles in melanocyte (patho)physiology, we present an overview on the differential effects of altered gravity (hypergravity/simulated microgravity) on the cGMP signaling pathway in melanocytes and melanoma cells with different metastatic potential. We believe that future experiments in real microgravity may benefit from considering cGMP signaling as a possible factor for melanocyte transformation and in medication.
High Cell Selectivity and Bactericidal Mechanism of Symmetric Peptides Centered on d-Pro–Gly Pairs
Authors: Jia Boyan , Wang Yiming , Zhang Ying , Wang Zi , Wang Xue , Muhammad Inam , Kong Lingcong , Pei Zhihua , Ma Hongxia , Jiang Xiuyun
Published: February 2020

Antimicrobial peptides (AMPs) have a unique action mechanism that can help to solve global problems in antibiotic resistance. However, their low therapeutic index and poor stability seriously hamper their development as therapeutic agents. In order to overcome these problems, we designed peptides based on the sequence template XXRXXRRzzRRXXRXX-NH2, where X represents a hydrophobic amino acid like Phe (F), Ile (I), and Leu (L), while zz represents Gly–Gly (GG) or d-Pro–Gly (pG). Showing effective antimicrobial activity against Gram-negative bacteria and low toxicity, designed peptides had a tendency to form an α-helical structure in membrane-mimetic environments. Among them, peptide LRpG (X: L, zz: pG) showed the highest geometric mean average treatment index (GMTI = 73.1), better salt, temperature and pH stability, and an additive effect with conventional antibiotics. Peptide LRpG played the role of anti-Gram-negative bacteria through destroying the cell membrane. In addition, peptide LRpG also exhibited an anti-inflammatory activity by effectively neutralizing endotoxin. Briefly, peptide LRpG has the potential to serve as a therapeutic agent to reduce antibiotic resistance owing to its high therapeutic index and great stability.
Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37
Authors: Nova Zuzana , Skovierova Henrieta , Strnadel Jan , Halasova Erika , Calkovska Andrea
Published: February 2020

Alveolar epithelial type II (ATII) cells and their proper function are essential for maintaining lung integrity and homeostasis. However, they can be damaged by lipopolysaccharide (LPS) during Gram-negative bacterial infection. Thus, this study evaluated and compared the effects of LPS on short and long-term cultures of A549 cells by determining the cell viability, levels of oxidative stress and antimicrobial peptide cathelicidin LL-37 and changes in the expression of surfactant proteins (SPs). Moreover, we compared A549 cell response to LPS in the presence of different serum concentrations. Additionally, the effect of N-acetylcysteine (NAC) on LPS-induced oxidative stress as a possible treatment was determined. Our results indicate that A549 cells are relatively resistant to LPS and able to maintain integrity even at high LPS concentrations. Their response to endotoxin is partially dependent on serum concentration. NAC failed to lower LPS-induced oxidative stress in A549 cells. Finally, LPS modulates SP gene expression in A549 cells in a time dependent manner and differences between short and long-term cultures were present. Our results support the idea that long-term cultivation of A549 cells could promote a more ATII-like phenotype and thus could be a more suitable model for ATII cells, especially for in vitro studies dealing with surfactant production.
Waste Rubber Recycling: A Review on the Evolution and Properties of Thermoplastic Elastomers
Authors: Fazli Ali , Rodrigue Denis
Published: February 2020

Currently, plastics and rubbers are broadly being used to produce a wide range of products for several applications like automotive, building and construction, material handling, packaging, toys, etc. However, their waste (materials after their end of life) do not degrade and remain for a long period of time in the environment. The increase of polymeric waste materials’ generation (plastics and rubbers) in the world led to the need to develop suitable methods to reuse these waste materials and decrease their negative effects by simple disposal into the environment. Combustion and landfilling as traditional methods of polymer waste elimination have several disadvantages such as the formation of dust, fumes, and toxic gases in the air, as well as pollution of underground water resources. From the point of energy consumption and environmental issues, polymer recycling is the most efficient way to manage these waste materials. In the case of rubber recycling, the waste rubber can go through size reduction, and the resulting powders can be melt blended with thermoplastic resins to produce thermoplastic elastomer (TPE) compounds. TPE are multi-functional polymeric materials combining the processability of thermoplastics and the elasticity of rubbers. However, these materials show poor mechanical performance as a result of the incompatibility and immiscibility of most polymer blends. Therefore, the main problem associated with TPE production from recycled materials via melt blending is the low affinity and interaction between the thermoplastic matrix and the crosslinked rubber. This leads to phase separation and weak adhesion between both phases. In this review, the latest developments related to recycled rubbers in TPE are presented, as well as the different compatibilisation methods used to improve the adhesion between waste rubbers and thermoplastic resins. Finally, a conclusion on the current situation is provided with openings for future works.
Antimicrobial Nanostructured Coatings: A Gas Phase Deposition and Magnetron Sputtering Perspective
Authors: Benetti Giulio , Cavaliere Emanuele , Banfi Francesco , Gavioli Luca
Published: February 2020

Counteracting the spreading of multi-drug-resistant pathogens, taking place through surface-mediated cross-contamination, is amongst the higher priorities in public health policies. For these reason an appropriate design of antimicrobial nanostructured coatings may allow to exploit different antimicrobial mechanisms pathways, to be specifically activated by tailoring the coatings composition and morphology. Furthermore, their mechanical properties are of the utmost importance in view of the antimicrobial surface durability. Indeed, the coating properties might be tuned differently according to the specific synthesis method. The present review focuses on nanoparticle based bactericidal coatings obtained via magneton-spattering and supersonic cluster beam deposition. The bacteria–NP interaction mechanisms are first reviewed, thus making clear the requirements that a nanoparticle-based film should meet in order to serve as a bactericidal coating. Paradigmatic examples of coatings, obtained by magnetron sputtering and supersonic cluster beam deposition, are discussed. The emphasis is on widening the bactericidal spectrum so as to be effective both against gram-positive and gram-negative bacteria, while ensuring a good adhesion to a variety of substrates and mechanical durability. It is discussed how this goal may be achieved combining different elements into the coating.
Low Temperature Synthesis of Phase Pure MoAlB Powder in Molten NaCl
Authors: Liu Cheng , Hou Zhaoping , Jia Quanli , Liu Xueyin , Zhang Shaowei
Published: February 2020

MoAlB fine powders were prepared in molten NaCl from Al, B and Mo powders. The effects of key parameters affecting the synthesis process and phase morphology were examined and the underpinning mechanisms proposed. MoAlB product particles exhibited different shapes/sizes, as follows: spherical grains (1~3 μm), plate-like particles (<5 μm in diameter) and columnar crystals with lengths up to 20 μm and diameters up to 5 μm, resultant from different reaction processes. Phase pure MoAlB was synthesised under the following optimal conditions: use of 140% excess Al and 6 h of firing at 1000 °C. This temperature was at least 100 °C lower than required by other methods/techniques previously reported. At the synthesis condition, Mo first reacted with Al and B, forming Al8Mo3 and MoB, respectively, which further reacted with excess Al to form Al-rich Al–Mo phases and MoAlB. The Al-rich Al–Mo phases further reacted with the residual B, forming additional MoAlB. The molten NaCl played an important role in accelerating the overall synthesis process.
Heavy Metals Removing from Municipal Solid Waste Incineration Fly Ashes by Electric Field-Enhanced Washing
Authors: Tian Yang , Wang Rong , Luo Zhenggang , Wang Rui , Yang Feihua , Wang Zhaojia , Shu Jiancheng , Chen Mengjun
Published: February 2020

Municipal solid waste incineration (MSWI) fly ash contains chlorides, heavy metals, and organic pollutants, which requires appropriate disposal to eliminate this risk. In this study, the effects of agents on heavy metals removal from MSWI fly ash by electric field-enhanced washing were systematically studied. The results show that when these fly ashes were washed at a current density of 35 mA/cm2, polarity switching frequency of 40 Hz, Ethylenediaminetetraacetic acid (EDTA) dosage of 0.5 mol/L, and a pH of 2 for 4 h, almost all of the Cd and Ni could be were removed, with a removal efficiency of 100.00% and 99.59%, respectively. Meanwhile, it also shows a significant effect on Cu and Zn, with a removal efficiency higher than 85%. After washing, the results of the sequential extraction procedure showed that the residual forms of Pb, Cu, Zn, Cd, Ni, and As increased obviously. According to GB5085.3-2007, the toxicity of the treated MSWI fly ash were below their thresholds of 5 and 1 mg/L for Pb and Cd, respectively. Thus, a novel technology for heavy metals removal from MSWI fly ash is proposed.
Results in wt-Distance over b-Metric Spaces
Authors: Karapınar Erdal , Chifu Cristian
Published: February 2020

In this manuscript, we introduce Meir-Keeler type contractions and Geraghty type contractions in the setting of the w t -distances over b-metric spaces. We examine the existence of a fixed point for such mappings. Under some additional assumption, we proved the uniqueness of the found fixed point.
Multi-Attribute Group Decision Making Based on Multigranulation Probabilistic Models with Interval-Valued Neutrosophic Information
Authors: Zhang Chao , Li Deyu , Kang Xiangping , Liang Yudong , Broumi Said , Sangaiah Arun Kumar
Published: February 2020

In plenty of realistic situations, multi-attribute group decision-making (MAGDM) is ubiquitous and significant in daily activities of individuals and organizations. Among diverse tools for coping with MAGDM, granular computing-based approaches constitute a series of viable and efficient theories by means of multi-view problem solving strategies. In this paper, in order to handle MAGDM issues with interval-valued neutrosophic (IN) information, we adopt one of the granular computing (GrC)-based approaches, known as multigranulation probabilistic models, to address IN MAGDM problems. More specifically, after revisiting the related fundamental knowledge, three types of IN multigranulation probabilistic models are designed at first. Then, some key properties of the developed theoretical models are explored. Afterwards, a MAGDM algorithm for merger and acquisition target selections (M&A TSs) with IN information is summed up. Finally, a real-life case study together with several detailed discussions is investigated to present the validity of the developed models.