bluuebunny/crossref_metadata_2025 huggingface dataset:
Quality of Life and Needs of Deaf Informal Caregivers of Loved Ones with Alzheimer’s and Related Dementia
Authors: Ammons Donalda , Engelman Alina , Kushalnagar Poorna
Published: October 2020

No studies have included the experiences and needs of informal caregivers who are deaf, use American Sign Language (ASL), and care for a loved one with Alzheimer’s disease or related dementias (ADRD). The CDC’s BRFSS Caregiver Module and PROMIS-Deaf Profile measures were administered via an online bilingual English/ASL platform between October 2019 and March 2020. Out of 194 deaf adult signers who completed an online survey, 42 respondents (mean age = 66; SD = 12; 74% White) endorsed informally caring for someone with a medical condition. In this survey subsample of informal caregivers, more years of education was significantly associated with higher generic quality of life and higher deaf-specific quality of life. A smaller subset of informal deaf informal caregivers who were currently taking care of loved ones with ADRD were then invited to participate in a semi-structured interview. Among the 22 informal caregivers who were interviewed, there was a strong agreement among the participants who felt that their quality of life as informal caregivers was worse than hearing informal caregivers who took care of loved ones with ADRD. Findings highlight the importance of a call to action to address the needs of deaf informal ADRD caregivers.
Envisioning Shared Decision Making: A Reflection for the Next Decade
Authors: Barton Jennifer L. , Kunneman Marleen , Hargraves Ian , LeBlanc Annie , Brito Juan P. , Scholl Isabelle , Montori Victor M.
Published: October 2020

Despite the evolving evidence in favor of shared decision making (SDM) and of decades-long calls for its adoption, SDM remains uncommon in routine care. Reflecting on this lack of progress, we sought to reimagine the future of SDM and the path to take us there. In late 2017, a multidisciplinary and international group of six researchers were challenged by a senior SDM scholar to envision the future and, based on a provocatively critical view of the present, to write letters to themselves from the year 2028. Letters were exchanged and discussed electronically. The group then met in person to discuss the letters. Since the letters painted a dystopian picture, they triggered questions about the nature of SDM, who should benefit from SDM, how to measure its contribution to care, and what new ways can be invented to design and test interventions to implement SDM in routine care. Through contrasting the purposefully generated dystopias with an ideal future for SDM, we generated reflections on a research agenda for SDM. These reflections hinged on recognizing SDM’s contributing to care, that is, as a way to advance the problematic human situation of patients. These focused on three distinct yet complimentary contributors to SDM: 1) the process of making decisions, 2) humanistic communication, and 3) fit-to-care of the resulting decision. The group then concluded that to move SDM from envisioned to routine practice, and to ensure it reaches all, particularly persons rendered vulnerable by current forms of health care, a substantial investment in implementation research is necessary. Perhaps the discussion of these reflections can contribute to a path forward that will improve the likelihood of the future we dream for SDM.
Decomposing the Gaps in Access to LPG across Socio-Religious Groups in Rural India
Authors: Singh Sudershan , Ranjan Rahul , Gonsalves Oliver Nelson
Published: October 2020

This article investigates the patterns of household cooking fuel choice and its determining factors for various socio-religious groups in rural India using NSS 68th Consumption Expenditure round. The article also studies how the inter-household gaps result in many levels of Liquefied Petroleum Gas (LPG) access for households belonging to various socio-religious groups. In this regard, the application of a logistic model for the considered socio-religious groups highlights the importance of income and the education level of different members of the households. Further, the differences in the probability of access to LPG among various socio-religious groups, with respect to upper caste Hindus, are decomposed using Fairlie decomposition method. The gap in income determines a major proportion of the gap, followed by the education level of the members. We also find that the Scheduled Tribes (STs) face the problem of availability of LPG, while other socio-religious groups, when compared to upper caste Hindus, either face affordability issues or possess a taste for traditional fuel.
Impacts of child welfare worker and clientele characteristics on attitudes toward trauma informed-care
Authors: Collin-Vézina Delphine , Brend Denise , Black Karen , Beeman Irene , Brown Steve
Published: October 2020

Background:

There is increasing recognition of the need to integrate trauma-informed care (TIC) into child welfare practices, given the high rates of trauma experiences among children and youth across these settings. The implementation of TIC is facilitated by various elements, including worker attitudes, yet further research is needed to illuminate the factors that influence child welfare workers’ positive regard for TIC.

Objectives:

This study aims to explore the relationship between child welfare worker attitudes regarding TIC with workers’ and clients’ individual characteristics.

Methods:

N = 418 child welfare workers from 11 agencies completed two measures: a demographic questionnaire as well as the French translated version of the ARTIC-35 questionnaire comprised of five subscales. Linear mixed effects models were run for each ARTIC subscale, examining how child and worker factors affect attitudes toward TIC.

Results:

Participants indicated relatively positive attitudes toward TIC. Managerial staff in offender units scored higher on the subscale regarding their beliefs about the causes underlying child behaviors and on the subscale regarding beliefs about the secondary effects of trauma, than their counterparts in protection units serving boys. Managers scored higher than frontline staff on worker self-efficacy, response to problem behavior, and on-the-job behavior subscales. Workers with a community college degree—and not a university degree–indicated greater sense of self-efficacy.

Conclusions:

This study points to the importance of paying attention to the characteristics of both workers and clients that may influence inclination toward TIC principles, as a means to build effective integration of this approach in child-serving settings.
Low serum free light chain is associated with risk of COPD exacerbation
Authors: Tanimura Kazuya , Sato Susumu , Sato Atsuyasu , Tanabe Naoya , Hasegawa Koichi , Uemasu Kiyoshi , Hamakawa Yoko , Hirai Toyohiro , Muro Shigeo
Published: October 2020

Background

Most exacerbations of chronic obstructive pulmonary disease (COPD) are triggered by respiratory tract infections. Adaptive immunityviaantibody production is important in preventing infections. Impaired antibody production is reported to be associated with an increased risk of exacerbations of COPD. In the present study, we elucidated whether reduced free light chains (FLCs), which are excessive amounts of light chains produced during antibody synthesis and can be used to estimate systemic antibody production, may be a promising biomarker to predict the risk of exacerbations of COPD.

Methods

We enrolled stable male patients with COPD and prospectively observed them for 2 years. At baseline, serum combined FLC (cFLC; sum of kappa and lambda values) and pulmonary function were evaluated. Exacerbation was defined as a worsening of symptoms requiring treatments with antibiotics, corticosteroids or both.

Results

63 patients with stable COPD were enrolled (72.8±8.1 years, GOLD A/B/C/D=24/28/6/5), and 51 patients completed the 2-year follow-up. Serum cFLC was 31.1 mg·L−1on average and ranged widely (1.4 to 89.9 mg·L−1). The patients with low cFLC (below the mean−sd, n=6) experienced a significantly shorter time to the first exacerbation of COPD (p<0.0001 by the log-rank test). A multivariate Cox proportional hazard model, including the COPD assessment test score, % predicted forced expiratory volume in 1 s (FEV1% pred), and number of previous exacerbations demonstrated that low cFLC and low FEV1% pred were independently and significantly correlated with the risk for exacerbations of COPD.

Conclusion

Low cFLC may be a B-cell-associated novel biomarker associated with risk of COPD exacerbation.
Influence of wood anatomy on fiber orientation measurement obtained by laser scanning on five European species
Authors: Besseau Benoît , Pot Guillaume , Collet Robert , Viguier Joffrey
Published: October 2020

In order to obtain high production rates of sawn timber, the sawmilling industry can use laser scanning, allowing knot detection and machine strength grading. In particular, laser scanners measure grain angle using the so-called tracheid effect on wood surface where an elliptic scattering of the laser light can be observed. This paper aims to describe the light scattering obtained by a laser beam projection on wood surface and to assess the accuracy of such fiber orientation measurement on five European species. Firstly, fiber orientation measurement error was assessed by rotating samples. Secondly, the description of the scattering effect was done considering ellipse axis ratios and areas. This was studied according to several parameters such as wood surface machining, moisture content, and orthotropic planes of symmetry. Fiber orientation measurement was successfully performed on all the tested species. The measurement error was below 1.6°, except for oak longitudinal–radial (LR) plane showing an error up to 3.1°. For most of the species, the error was higher in LR plane because of the influence of medullary rays. Despite the observation of major variabilities in laser light scattering, it was possible to measure the grain angles with a good accuracy for all investigated species.
A method for estimating coherence of molecular mechanisms in major human disease and traits
Authors: Dozmorov Mikhail G. , Cresswell Kellen G. , Bacanu Silviu-Alin , Craver Carl , Reimers Mark , Kendler Kenneth S.
Published: October 2020

Background

Phenotypes such as height and intelligence, are thought to be a product of the collective effects of multiple phenotype-associated genes and interactions among their protein products. High/low degree of interactions is suggestive of coherent/random molecular mechanisms, respectively. Comparing the degree of interactions may help to better understand the coherence of phenotype-specific molecular mechanisms and the potential for therapeutic intervention.
However, direct comparison of the degree of interactions is difficult due to different sizes and configurations of phenotype-associated gene networks.

Methods

We introduce a metric for measuring coherence of molecular-interaction networks as a slope of internal versus external distributions of the degree of interactions. The internal degree distribution is defined by interaction counts within a phenotype-specific gene network, while the external degree distribution counts interactions with other genes in the whole protein–protein interaction (PPI) network. We present a novel method for normalizing the coherence estimates, making them directly comparable.

Results

Using STRING and BioGrid PPI databases, we compared the coherence of 116 phenotype-associated gene sets from GWAScatalog against size-matched KEGG pathways (the reference for high coherence) and random networks (the lower limit of coherence). We observed a range of coherence estimates for each category of phenotypes. Metabolic traits and diseases were the most coherent, while psychiatric disorders and intelligence-related traits were the least coherent. We demonstrate that coherence and modularity measures capture distinct network properties.

Conclusions

We present a general-purpose method for estimating and comparing the coherence of molecular-interaction gene networks that accounts for the network size and shape differences. Our results highlight gaps in our current knowledge of genetics and molecular mechanisms of complex phenotypes and suggest priorities for future GWASs.
Interleukin 4 gene polymorphism (−589C/T) and the risk of asthma: a meta-analysis and met-regression based on 55 studies
Authors: Kousha Ahmad , Mahdavi Gorabi Armita , Forouzesh Mehdi , Hosseini Mojgan , Alexander Markov , Imani Danyal , Razi Bahman , Mousavi Mohammad Javad , Aslani Saeed , Mikaeili Haleh
Published: October 2020

Background

Numerous investigations have previously evaluated the association of interleukin (IL) 4 gene polymorphisms and the risk of asthma, conferring inconsistent results. To resolve the incongruent outcomes yielded from different single studies, we conducted the most up-to-date meta-analysis of IL4 gene −589C/T (rs2243250) polymorphism and susceptibility to asthma.

Methods

A systematic literature search was performed in ISI web of science, Scopus, Medline/PubMed databases prior to September 2020, and the pooled odds ratio (OR) and their corresponding 95% CI were calculated to determine the association strength.

Results

Literature search led to retrieving of 49 publications (55 case-control studies) containing 9572 cases and 9881 controls. It was revealed that IL4 gene −589C/T polymorphism increased the risk of asthma across all genetic models, including dominant model (OR = 1.22), recessive model (OR = 1.17), allelic model (OR = 1.21), and TT vs. CC model (OR = 1.34), but not the CT vs. TT model. The subgroup analysis by age indicated that IL4 gene -589C/T polymorphism was significantly associated with asthma risk in both pediatrics and adults. Additionally, the subgroup analysis by ethnicity revealed significant association in Asian, American, and Europeans. Finally, subgroup analysis by East Asian and non-East Asian populations indicated significant associations.

Conclusions

The current meta-analysis revealed that IL4 gene -589C/T polymorphism was a susceptibility risk in both pediatrics and adults in the whole and different ethnic groups.
Evaluating the efficacy of an Advanced Care Planning Program for Health Decisions in patients with advanced heart failure: protocol for a Randomized Clinical Trial
Authors: Sánchez Beatriz , Guijarro Carlos , Velasco María , Vicente María Jesús , Galán Miguel , Herreros Benjamín
Published: October 2020

Background

An Advanced Care Planning (ACP) program of health decisions is the result of a process of reflection and relationship-building between the patient, their relatives and health professionals. It is based on respect for patients’ autonomy, involving them in making decisions about their disease in a way that is shared between the medical team, the patient and their relatives. Up until now, the efficacy of an ACP has not been measured in the existing literature, and therefore it is unknown if these programs reach their goal. The main objective of our study is to evaluate the efficacy of an ACP program for decision-making in patients with advanced heart failure (HF) in comparison to usual follow up and care. This objective will be evaluated by the Patient Activation Measure test, which measures the participation and self-management of the patient in decision-making. Secondary objectives: to evaluate the effect of the program on quality of life, to know if the patients wishes expressed through the ACP program are fulfilled, to measure the impact of the program on patients’ caregivers, to determine the satisfaction of patients included in the program and to evaluate the effect on quality of death.

Methods

Randomized multicentre clinical trial at four hospitals in Madrid. Once they are included in the study, patients’ allocation to groups (control vs intervention) will be made by alternative sampling. ACP will be applied to the intervention group, whereas in the Control Group usual follow-up will be carried out in HF units. All patients will fulfil questionnaires and tests related to the objectives of the study again after a 12-month follow-up period in order to gauge the effect of ACP in patients with advanced HF.

Discussion

The characteristics of patients with advanced HF make them a model for designing ACP programs, given the high prevalence of this disease, the progressive increase in its incidence and it’s clinical characteristics. Until now, the efficacy of this type of program has not been measured, so this Clinical Trial can provide relevant data for future ACP projects.

Trial registration ClinicalTrials.gov Identifier: NCT04424680. Registered 9 June 2020. Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT04424680?term=NCT04424680&draw=2&rank=1.
Association between attendance at outpatient follow-up appointments and blood pressure control among patients with hypertension
Authors: Mahmood Sajid , Jalal Zahraa , Hadi Muhammad Abdul , Shah Kifayat Ullah
Published: October 2020

Objective

The aim of this study was to assess the impact of regularity in treatment follow-up appointments on treatment outcomes among hypertensive patients attending different healthcare settings in Islamabad, Pakistan. Additionally, factors associated with regularity in treatment follow-up were also identified.

Methods

A cross-sectional study was undertaken in selected primary, secondary and tertiary healthcare settings between September, 2017 and December, 2018 in Islamabad, Pakistan. A structured data collection form was used to gather sociodemographic and clinical data of recruited patients. Binary logistic regression analyses were undertaken to determine association between regularity in treatment follow-up appointments and blood pressure control and to determine covariates significantly associated with regularity in treatment follow-up appointments.

Results

A total of 662 patients with hypertension participated in the study. More than half 346 (52%) of the patients were females. The mean age of participants was 54 ± 12 years. Only 274 (41%) patients regularly attended treatment follow-up appointments. Regression analysis found that regular treatment follow-up was an independent predictor of controlled blood pressure (OR 1.561 [95% CI 1.102–2.211;P = 0.024]). Gender (OR 1.720 [95% CI 1.259–2.350;P = 0.001]), age (OR 1.462 [CI 95%:1.059–2.020;P = 0.021]), higher education (OR 1.7 [95% CI 1.041–2.778;P = 0.034]), entitlement to free medical care (OR 3.166 [95% CI 2.284–4.388;P = 0.0001]), treatment duration (OR 1.788 [95% CI 1.288–2.483;P = 0.001]), number of medications (OR 1.585 [95% CI 1.259–1.996;P = 0.0001]), presence of co-morbidity (OR 3.214 [95% CI 2.248–4.593;P = 0.0001]) and medication adherence (OR 6.231 [95% CI 4.264–9.106;P = 0.0001]) were significantly associated with regularity in treatment follow-up appointments.

Conclusion

Attendance at follow-up visits was alarmingly low among patients with hypertension in Pakistan which may explain poor treatment outcomes in patients. Evidence-based targeted interventions should be developed and implemented, considering local needs, to improve attendance at treatment follow-up appointments.
Train the brain with music (TBM): brain plasticity and cognitive benefits induced by musical training in elderly people in Germany and Switzerland, a study protocol for an RCT comparing musical instrumental practice to sensitization to music
Authors: James Clara E. , Altenmüller Eckart , Kliegel Matthias , Krüger Tillmann H.C. , Van De Ville Dimitri , Worschech Florian , Abdili Laura , Scholz Daniel S. , Jünemann Kristin , Hering Alexandra , Grouiller Frédéric , Sinke Christopher , Marie Damien
Published: October 2020

Background

Recent data suggest that musical practice prevents age-related cognitive decline. But experimental evidence remains sparse and no concise information on the neurophysiological bases exists, although cognitive decline represents a major impediment to healthy aging. A challenge in the field of aging is developing training regimens that stimulate neuroplasticity and delay or reverse symptoms of cognitive and cerebral decline. To be successful, these regimens should be easily integrated in daily life and intrinsically motivating. This study combines for the first-time protocolled music practice in elderly with cutting-edge neuroimaging and behavioral approaches, comparing two types of musical education.

Methods

We conduct a two-site Hannover-Geneva randomized intervention study in altogether 155 retired healthy elderly (64–78) years, (63 in Geneva, 92 in Hannover), offering either piano instruction (experimental group) or musical listening awareness (control group). Over 12 months all participants receive weekly training for 1 hour, and exercise at home for ~ 30 min daily. Both groups study different music styles. Participants are tested at 4 time points (0, 6, and 12 months & post-training (18 months)) on cognitive and perceptual-motor aptitudes as well as via wide-ranging functional and structural neuroimaging and blood sampling.

Discussion

We aim to demonstrate positive transfer effects for faculties traditionally described to decline with age, particularly in the piano group: executive functions, working memory, processing speed, abstract thinking and fine motor skills. Benefits in both groups may show for verbal memory, hearing in noise and subjective well-being. In association with these behavioral benefits we anticipate functional and structural brain plasticity in temporal (medial and lateral), prefrontal and parietal areas and the basal ganglia.

We intend exhibiting for the first time that musical activities can provoke important societal impacts by diminishing cognitive and perceptual-motor decline supported by functional and structural brain plasticity.

Trial registration

The Ethikkomission of the Leibniz Universität Hannover approved the protocol on 14.08.17 (no. 3604–2017), the neuroimaging part and blood sampling was approved by the Hannover Medical School on 07.03.18. The full protocol was approved by the Commission cantonale d’éthique de la recherche de Genève (no. 2016–02224) on 27.02.18 and registered atclinicaltrials.govon 17.09.18 (NCT03674931, no. 81185).
Two novel PCDH19 mutations in Russian patients with epilepsy with intellectual disability limited to females: a case report
Authors: Kozina Anastasiya Aleksandrovna , Okuneva Elena Grigorievna , Baryshnikova Natalia Vladimirovna , Fedonyuk Inessa Dmitrievna , Kholin Alexey Aleksandrovich , Il’ina Elena Stepanovna , Krasnenko Anna Yurievna , Stetsenko Ivan Fedorovich , Plotnikov Nikolay Alekseevich , Klimchuk Olesia Igorevna , Surkova Ekaterina Ivanovna , Ilinsky Valery Vladimirovich
Published: October 2020

Background

Epilepsy with intellectual disability limited to females (Epileptic encephalopathy, early infantile, 9; EIEE9) is a rare early infantile epileptic encephalopathy characterized by an unusual X-linked inheritance: females with heterozygous mutations are affected, while hemizygous males are not.

Case presentation

We describe the clinical and molecular characteristics of 2 Russian patients with EIEE9 (females, ages 3 years and 7 years). In these patients seizures developed at the age of 3 years. Additionally, for our patients and for cases described in the literature we searched for a possible relationship between the type and localization of the mutation and the EIEE9 clinical phenotype.

Conclusions

We identified two novel PCDH19 mutations in EIEE9 patients: a missense mutation in exon 1 (c.1236C > A, p.Asp412Glu) and a frameshift in exon 3 (c.2386_2387insGTCT, p.Thr796fs). We conclude that the age of seizure onset and the presence of intellectual disability may depend not on the type and localization of PCDH19 mutations, but on the X-inactivation status. The study also highlights the need to screen for EIEE9 among young female epilepsy patients.
Extracranial metastases in secondary glioblastoma multiforme: a case report
Authors: Rossi Jessica , Giaccherini Lucia , Cavallieri Francesco , Napoli Manuela , Moratti Claudio , Froio Elisabetta , Serra Silvia , Fraternali Alessandro , Ghadirpour Reza , Cozzi Salvatore , Ciammella Patrizia , Iaccarino Corrado , Pascarella Rosario , Valzania Franco , Pisanello Anna
Published: October 2020

Background

Glioblastoma (GBM) is known for its devastating intracranial infiltration and its unfavorable prognosis, while extracranial involvement is a very rare event, more commonly attributed to IDH wild-type (primary) GBM evolution.

Case presentation

We present a case of a young woman with a World Health Organization (WHO) grade II Astrocytoma evolved to WHO grade IV IDH mutant glioblastoma, with subsequent development of lymphatic and bone metastases, despite the favorable biomolecular pattern and the stability of the primary brain lesion.

Conclusions

Our case highlights that grade II Astrocytoma may evolve to a GBM and rarely lead to a secondary metastatic diffusion, which can progress quite rapidly; any symptoms referable to a possible systemic involvement should be carefully investigated.
A qualitative study of the work experiences of midwives performing obstetric ultrasound in Norway
Authors: Reiso Magnhild , Langli Berit , Sommerseth Eva , Johannessen Aud
Published: October 2020

Background

Performing obstetric ultrasound is part of midwifery practice in Norway. Knowledge of these midwives’ working situation can enhance understanding of what their work involves and the challenges they encounter in their practice. The aim of this study was to gain insight into how midwife sonographers perceive their work in obstetric ultrasound.

Methods

A qualitative study with individual interviews was conducted in 2018. Midwives (n = 13) with a postgraduate ultrasound qualification who performed obstetric ultrasound in private clinics and/or the public health sector were included. All four regional health authorities in Norway were represented. The data gathered were analysed using content analysis.

Results

The analysis resulted in three main themes. (1) Working as a midwife sonographer involves a holistic approach. By practising their competence, in both midwifery and sonography, they could answer questions and reassure pregnant women. The participants also had a feeling of great responsibility in their work. (2) Being part of a professional environment in obstetric ultrasound was important for professional interaction, belonging and learning. (3) Developing and maintaining competence as a midwife sonographer had a positive influence on midwives’ motivation and confidence, and allowed for more variety in their work.

Conclusions

Holistic care of the pregnant woman, her partner and the unborn baby was an important part of the participants’ work. They wanted to meet colleagues within their field, develop their expertise and have influence over their work situation. Organizational factors seemed to affect the participants’ overall ability to practise their skills and thus also their job satisfaction.
Improved personalized survival prediction of patients with diffuse large B-cell Lymphoma using gene expression profiling
Authors: Mosquera Orgueira Adrián , Díaz Arias José Ángel , Cid López Miguel , Peleteiro Raíndo Andrés , Antelo Rodríguez Beatriz , Aliste Santos Carlos , Alonso Vence Natalia , Bendaña López Ángeles , Abuín Blanco Aitor , Bao Pérez Laura , González Pérez Marta Sonia , Pérez Encinas Manuel Mateo , Fraga Rodríguez Máximo Francisco , Bello López José Luis
Published: October 2020

Background

Thirty to forty percent of patients with Diffuse Large B-cell Lymphoma (DLBCL) have an adverse clinical evolution. The increased understanding of DLBCL biology has shed light on the clinical evolution of this pathology, leading to the discovery of prognostic factors based on gene expression data, genomic rearrangements and mutational subgroups. Nevertheless, additional efforts are needed in order to enable survival predictions at the patient level. In this study we investigated new machine learning-based models of survival using transcriptomic and clinical data.

Methods

Gene expression profiling (GEP) of in 2 different publicly available retrospective DLBCL cohorts were analyzed. Cox regression and unsupervised clustering were performed in order to identify probes associated with overall survival on the largest cohort. Random forests were created to model survival using combinations of GEP data, COO classification and clinical information. Cross-validation was used to compare model results in the training set, and Harrel’s concordance index (c-index) was used to assess model’s predictability. Results were validated in an independent test set.

Results

Two hundred thirty-three and sixty-four patients were included in the training and test set, respectively. Initially we derived and validated a 4-gene expression clusterization that was independently associated with lower survival in 20% of patients. This pattern included the following genes: TNFRSF9, BIRC3, BCL2L1 and G3BP2. Thereafter, we applied machine-learning models to predict survival. A set of 102 genes was highly predictive of disease outcome, outperforming available clinical information and COO classification. The final best model integrated clinical information, COO classification, 4-gene-based clusterization and the expression levels of 50 individual genes (training set c-index, 0.8404, test set c-index, 0.7942).

Conclusion

Our results indicate that DLBCL survival models based on the application of machine learning algorithms to gene expression and clinical data can largely outperform other important prognostic variables such as disease stage and COO. Head-to-head comparisons with other risk stratification models are needed to compare its usefulness.
Cell type-specific genotoxicity in estrogen-exposed ovarian and fallopian epithelium
Authors: Song Liang , Tang Zizhi , Peng Changsheng , Yang Yueming , Guo Chang , Wang Danqing , Guo Liandi , Chen Jie , Liu Cong
Published: October 2020

Background

Loss of the genomic stability jeopardize genome stability and promote malignancies. A fraction of ovarian cancer (OvCa) arises from pathological mutations of DNA repair genes that result in highly mutagenic genomes. However, it remains elusive why the ovarian epithelial cells are particularly susceptible to the malfunction of genome surveillance system.

Methods

To explore the genotoxic responses in the unique context of microenvironment for ovarian epithelium that is periodically exposed to high-level steroid hormones, we examined estrogen-induced DNA damage by immunofluorescence in OvCa cell lines, animal and human samples.

Results

We found that OvCa cells are burdened with high levels of endogenous DNA damage that is not correlated with genomic replication. The elevation of damage burden is attributable to the excessive concentration of bioactive estrogen instead of its chemomimetic derivative (tamoxifen). Induction of DNA lesions by estrogen is dependent on the expression of hormone receptors, and occurs in G1 and non-G1 phases of cell cycle. Moreover, depletion of homologous recombination (HR) genes (BRCA1 and BRCA2) exacerbated the genotoxicity of estrogen, highlighting the role of HR to counteract hormone-induced genome instability. Finally, the estrogen-induced DNA damage was reproduced in the epithelial compartments of both ovarian and fallopian tubes.

Conclusions

Taken together, our study disclose that estrogen-induced genotoxicity and HR deficiency perturb the genome stability of ovarian and fallopian epithelial cells, representing microenvironmental and genetic risk factors, respectively.
Endostar continuous versus intermittent intravenous infusion combined with chemotherapy for advanced NSCLC: a systematic review and meta-analysis including non-randomized studies
Authors: Wang Bo , Xu Lu , Li Qihuan , Man Sailimai , Jin Cheng , Liu Lian , Zhan Siyan , Ning Yi
Published: October 2020

Background

Both intermittent intravenous (IIV) infusion and continuous intravenous (CIV) infusion of Endostar are widely used for NSCLC in China. We aimed to compare the efficacy and safety of CIV of Endostar versus IIV in combination with first-line chemotherapy for patients with advanced NSCLC.

Methods

RCTs, NRCTs and cohort studies which compared CIV of Endostar with IIV in advanced NSCLC patients and reported efficacy or safety outcomes were eligible. Two reviewers independently screened records, extracted data and assessed risk of bias. Pooled risk ratios (RRs) with 95% confidence intervals were calculated using random effects meta-analysis for short-term efficacy and safety outcomes, and hazard ratios (HRs) for survival outcomes.

Results

Finally nine studies involving 597 patients were included, containing two RCTs, three NRCTs and four cohort studies. For short-term efficacy, moderate quality of evidence showed that there were no significant differences between CIV of Endostar and IIV in objective response rate (ORR; RR 1.34, 95% CI 0.91–1.98, P = 0.14) and disease control rate (DCR; RR 1.11, 95% CI 0.94–1.30, P = 0.21). Very low quality of evidence indicated that CIV of Endostar significantly improved both overall survival (OS; HR 0.69, 95% CI 0.48–0.99, P = 0.046) and progression-free survival (PFS; HR 0.71, 95% CI 0.55–0.93, P = 0.01) compared with IIV. As for safety outcomes, moderate quality of evidence found that CIV of Endostar significantly reduced the risk of myelosuppression (RR 0.55, 95% CI 0.32–0.96, P = 0.03) and cardiovascular toxicity (RR 0.21, 95% CI 0.06–0.78, P = 0.02) compared with IIV.

Conclusions

In advanced NSCLC, compared with IIV, CIV of Endostar had similar short-term efficacy, and substantially lower risk of myelosuppression and cardiovascular toxicity. Although very low quality of evidence supported the survival benefit of CIV compared with IIV, large RCTs with long-term follow-up are needed to demonstrate survival benefits. Caution should be given for off-label use of CIV of Endostar.
The evaluation of juvenile ocular hypertension by optical coherence tomography angiography
Authors: Chen Xiaoxiao , Wang Xiaolei , Hu Xinxin , Sun Xinghuai
Published: October 2020

Background

Vessel density (VD) of the elderly ocular hypertension patients measured by optical coherence tomography angiography (OCTA) have been reported. However, the studies of VD in juvenile ocular hypertension (JOHT) are limited. We wished to evaluate VD changes using OCTA in JOHT. We also investigated the potential risk parameters of intraocular pressure (IOP) and vertical cup/disc ratio (CDR) with OCTA for observing the development of JOHT.

Methods

We examined 86 eyes in 45 control (Ctrl) subjects and 65 eyes in 34 patients with JOHT using OCTA at the glaucoma clinic of the Eye, Ear, Nose, and Throat Hospital of Fudan University. The VD of radial peripapillary capillaries (RPC) and the perifoveal superficial vascular plexus (SVP) was compared between the Ctrl and JOHT groups. Other basic study factors such as age, sex, blood pressure, best-corrected visual acuity, central corneal thickness, IOP, CDR, the thickness of the retinal nerve fiber layer, ganglion cell complex, visual field mean deviation, and pattern standard deviation were also recorded.

Results

Bare difference was found in the nasal-inferior and temporal RPC-VD between the Ctrl and JOHT groups (P = 0.042 and P = 0.033, respectively) while SVP-VD was not (all P > 0.05). In the mixed linear regression model analysis, temporal RPC-VD was marginally negatively associated with high IOP (r = − 1.379, P = 0.043). Five additional sections of nasal, inferior-nasal, inferior-temporal, superior-temporal, and superior-nasal RPC-VD showed positive correlation with large CDR (all P <  0.05). SVP-VD in the superior and nasal regions was marginally negatively correlated with high IOP (r = − 1.877, P = 0.023; r = − 1.693, P = 0.049). No other regions were found statistical different of relationship between IOP, CDR and VD.

Conclusions

Nasal-inferior and temporal peripapillary VD was marginally lower in JOHT subjects. Regarding parameters of IOP and CDR, peripapillary temporal VD had a borderline level of negative correlation with IOP more than 21 mmHg while additional five regions were strongly positively correlated with large CDR. Some macular regions only found marginal positive correlation with parameter of high IOP. We conclude that OCTA can be used as a potential technique to evaluate the VD in JOHT and peripapillary scans should be analyzed individually based on different levels of CDR.
Airborne particulate matter, population mobility and COVID-19: a multi-city study in China
Authors: Wang Bo , Liu Jiangtao , Li Yanlin , Fu Shihua , Xu Xiaocheng , Li Lanyu , Zhou Ji , Liu Xingrong , He Xiaotao , Yan Jun , Shi Yanjun , Niu Jingping , Yang Yong , Li Yiyao , Luo Bin , Zhang Kai
Published: October 2020

Background

Coronavirus disease 2019 (COVID-19) is an emerging infectious disease, which has caused numerous deaths and health problems worldwide. This study aims to examine the effects of airborne particulate matter (PM) pollution and population mobility on COVID-19 across China.

Methods

We obtained daily confirmed cases of COVID-19, air particulate matter (PM2.5, PM10), weather parameters such as ambient temperature (AT) and absolute humidity (AH), and population mobility scale index (MSI) in 63 cities of China on a daily basis (excluding Wuhan) from January 01 to March 02, 2020. Then, the Generalized additive models (GAM) with a quasi-Poisson distribution were fitted to estimate the effects of PM10, PM2.5 and MSI on daily confirmed COVID-19 cases.

Results

We found each 1 unit increase in daily MSI was significantly positively associated with daily confirmed cases of COVID-19 in all lag days and the strongest estimated RR (1.21, 95% CIs:1.14 ~ 1.28) was observed at lag 014. In PM analysis, we found each 10 μg/m3 increase in the concentration of PM10 and PM2.5 was positively associated with the confirmed cases of COVID-19, and the estimated strongest RRs (both at lag 7) were 1.05 (95% CIs: 1.04, 1.07) and 1.06 (95% CIs: 1.04, 1.07), respectively. A similar trend was also found in all cumulative lag periods (from lag 01 to lag 014). The strongest effects for both PM10 and PM2.5 were at lag 014, and the RRs of each 10 μg/m3 increase were 1.18 (95% CIs:1.14, 1.22) and 1.23 (95% CIs:1.18, 1.29), respectively.

Conclusions

Population mobility and airborne particulate matter may be associated with an increased risk of COVID-19 transmission.
Undergraduate students’ norms for the Chinese version of the symptom check-List-90-R (SCL-90-R)
Authors: Yu Yulan , Wan Chonghua , Zhao Xudong , Huebner E. Scott , Tan Jianfeng , Xu Chuanzhi , Zhang Jingjing
Published: October 2020

Background

Despite widespread application of the Symptom Check-List-90-R (SCL-90-R) for Chinese undergraduate students, there are no appropriate norms for them. The aim of this study is to provide norms for the Chinese version of the tool for undergraduate students using a large and representative sample.

Methods

Four thousand eight hundred sixty students completed the scale of SCL-90. The mean scores obtained in the present study were compared with mean scores from previous normative samples.

Results

The mean scores for nine subscales of the SCL-90-R ranged from (1.36 ± 0.46) ~ (1.77 ± 0.63) and the mean (standard deviation)Global Severity Index(GSI) was 1.50 (0.49). Relative to previous normative studies, the findings suggested that Chinese undergraduate students’ self-reported mental health symptoms decreased in interpersonal sensitivity, depression, hostility, and paranoid ideation subscales.

Conclusion

It is necessary to revise the norms of the Chinese version of the SCL-90-R for undergraduate students.
Chinese public’s knowledge, perceived severity, and perceived controllability of COVID-19 and their associations with emotional and behavioural reactions, social participation, and precautionary behaviour: a national survey
Authors: Li Jian-Bin , Yang An , Dou Kai , Wang Lin-Xin , Zhang Ming-Chen , Lin Xiao-Qi
Published: October 2020

Background

The outbreak of the coronavirus disease-19 (COVID-19) has caused enormous stress among the public in China. Intellectual input from various aspects is needed to fight against COVID-19, including understanding of the public’s emotion and behaviour and their antecedents from the psychological perspectives. Drawing upon the cognitive appraisal theory, this study examined three cognitive appraisals (i.e., perceived severity, perceived controllability, and knowledge of COVID-19) and their associations with a wide range of emotional and behavioural outcomes among the Chinese public.

Methods

Participants were 4607 citizens (age range: 17–90 years, Mage = 23.71 years) from 31 provinces in China and they took part in a cross-sectional survey online.

Results

The results showed that the public’s emotional and behavioural reactions were slightly affected by the outbreak of COVID-19. Moreover, the public had limited participation in the events regarding COVID-19 but actively engaged in precautionary behaviour. In addition, results of structural equation model with latent variables revealed that the three appraisals were differentially related to the outcome variables (i.e., negative emotion, positive emotion, sleep problems, aggression, substance use, mobile phone use, social participation, and precautionary behaviour).

Conclusions

The findings highlight the utility of cognitive appraisal, as a core process of coping stress, in explaining the public’s emotion and behaviour in the encounter of public health concern. Practically, the findings facilitate the government and practitioners to design and deliver targeted intervention programs to the public.
The intestinal microbiome, weight, and metabolic changes in women treated by adjuvant chemotherapy for breast and gynecological malignancies
Authors: Uzan-Yulzari Atara , Morr Maya , Tareef-Nabwani Hala , Ziv Oren , Magid-Neriya Dafna , Armoni Ran , Muller Efrat , Leibovici Anca , Borenstein Elhanan , Louzoun Yoram , Shai Ayelet , Koren Omry
Published: October 2020

Background

Adjuvant chemotherapy induces weight gain, glucose intolerance, and hypertension in about a third of women. The mechanisms underlying these events have not been defined. This study assessed the association between the microbiome and weight gain in patients treated with adjuvant chemotherapy for breast and gynecological cancers.

Methods

Patients were recruited before starting adjuvant therapy. Weight and height were measured before treatment and 4–6 weeks after treatment completion. Weight gain was defined as an increase of 3% or more in body weight. A stool sample was collected before treatment, and 16S rRNA gene sequencing was performed. Data regarding oncological therapy, menopausal status, and antibiotic use was prospectively collected. Patients were excluded if they were treated by antibiotics during the study. Fecal transplant experiments from patients were conducted using Swiss Webster germ-free mice.

Results

Thirty-three patients were recruited; of them, 9 gained 3.5–10.6% of baseline weight. The pretreatment microbiome of women who gained weight following treatment was significantly different in diversity and taxonomy from that of control women. Fecal microbiota transplantation from pretreatment samples of patients that gained weight induced metabolic changes in germ-free mice compared to mice transplanted with pretreatment fecal samples from the control women.

Conclusion

The microbiome composition is predictive of weight gain following adjuvant chemotherapy and induces adverse metabolic changes in germ-free mice, suggesting it contributes to adverse metabolic changes seen in patients. Confirmation of these results in a larger patient cohort is warranted.
Clinical, MRI, and histopathological findings of congenital focal diplomyelia at the level of L4 in a female crossbred calf
Authors: Wunderink Gerlinde J. , Bergwerff Ursula E. A. , Vos Victoria R. , Delany Mark W. , Willems Dorien S. , Hut Peter R.
Published: October 2020

Background

This case report describes the clinical signs of a calf with focal diplomyelia at the level of the fourth lumbar vertebra. Magnetic resonance imaging (MRI) images and histological findings of the affected spinal cord are included in this case report. This case differs from previously reported cases in terms of localization and minimal extent of the congenital anomaly, clinical symptoms and findings during further examinations.

Case presentation

The calf was presented to the Farm Animal Health clinic, Faculty of Veterinary Medicine, Utrecht University, with an abnormal, stiff, ‘bunny-hop’ gait of the pelvic limbs. Prominent clinical findings included general proprioceptive ataxia with paraparesis, pathological spinal reflexes of the pelvic limbs and pollakiuria. MRI revealed a focal dilated central canal, and mid-sagittal T2 hyperintense band in the dorsal part of the spinal cord at the level of the third to fourth lumbar vertebra. By means of histology, the calf was diagnosed with focal diplomyelia at the level of the fourth lumbar vertebra, a rare congenital malformation of the spinal cord. The calf tested positive for Schmallenberg virus antibodies, however this is not considered to be part of the pathogenesis of the diplomyelia.

Conclusions

This case report adds value to future clinical practice, as it provides a clear description of focal diplomyelia as a previously unreported lesion and details its diagnosis using advanced imaging and histology. This type of lesion should be included in the differential diagnoses when a calf is presented with a general proprioceptive ataxia of the hind limbs. In particular, a ‘bunny-hop’ gait of the pelvic limbs is thought to be a specific clinical symptom of diplomyelia. This case report is of clinical and scientific importance as it demonstrates the possibility of a focal microscopic diplomyelia, which would not be evident by gross examination alone, as a cause of hind-limb ataxia. The aetiology of diplomyelia in calves remains unclear.
Stage-specific protein-domain mutational profile of invasive ductal breast cancer
Authors: Yu Ting , Choi Kwok Pui , Chen Ee Sin , Zhang Louxin
Published: October 2020

Background

Understanding the mechanisms underlying the malignant progression of cancer cells is crucial for early diagnosis and therapeutic treatment for cancer. Mutational heterogeneity of breast cancer suggests that about a dozen of cancer genes consistently mutate, together with many other genes mutating occasionally, in patients.

Methods

Using the whole-exome sequences and clinical information of 468 patients in the TCGA project data portal, we analyzed mutated protein domains and signaling pathway alterations in order to understand how infrequent mutations contribute aggregately to tumor progression in different stages.

Results

Our findings suggest that while the spectrum of mutated domains was diverse, mutations were aggregated in Pkinase, Pkinase Tyr, Y-Phosphatase and Src-homology 2 domains, highlighting the genetic heterogeneity in activating the protein tyrosine kinase signaling pathways in invasive ductal breast cancer.

Conclusions

The study provides new clues to the functional role of infrequent mutations in protein domain regions in different stages for invasive ductal breast cancer, yielding biological insights into metastasis for invasive ductal breast cancer.