bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlates of Objectively Measured Sitting Time in South Korean Adults: 2014–2015 Korea National Health and Nutrition Examination Survey
Authors: Lee Hyo , Lee Miyoung
Published: May 2022

The purpose of this study was to investigate daily amounts of time spent sitting and frequency of breaks from sitting and to identify their sociodemographic, environmental, and health behavioral correlates for Korean adults (age = 19–65). This study analyzed accelerometer subdata from the 2014–2015 Korea National Health and Nutrition Examination Survey (n = 1,768). Ordinary least squares regression models stratified by weekday and weekend were tested to identify correlates of time spent sitting and number of sitting breaks. The average daily amounts of sitting time during weekdays and weekends were 500.63 min (95% confidence interval [CI] = 495.20–506.06) and 488.10 min (95% CI = 481.72–494.49), respectively. On weekdays and weekends, the average numbers of breaks from sitting per hour were 6.62 (95% CI = 6.57–6.68) and 6.60 (95% CI = 6.54–6.66), respectively. The participants with the greatest daily sitting time tended to be male, middle-aged, never married, office workers, and residents of a metropolis; tended to have a high school educational level or higher; and had never smoked, were underweight, were physically inactive, and slept <6 h a day. Fewer breaks from sitting was associated with being male, never married, middle-aged, an office worker, an apartment resident, never having smoked, and underweight. Higher education level and physical inactivity were associated with more frequent breaks from sitting. To reduce sedentary behavior, this study helps identify at-risk populations and their characteristics. Future studies should incorporate longitudinal data and measure domain-specific sedentary behavior.
Effects of Short Video Addiction on the Motivation and Well-Being of Chinese Vocational College Students
Authors: Ye Jian-Hong , Wu Yu-Tai , Wu Yu-Feng , Chen Mei-Yen , Ye Jhen-Ni
Published: May 2022

While media use can be beneficial in some ways, excessive use of media has led to growing concerns about its potential negative consequences. With the popularity of Chinese video applications (apps) such as DouYin, TikTok, Kwai, and other short video apps sweeping through schools around the world. Due to the diversity and immersion principle of short videos, their popularity continues to grow, and the phenomenon of students being addicted to short videos also brings many hidden dangers to the learning effect. Among other things, the problem of excessive use of the Internet among Chinese youth has led the government to propose a series of control policies to strengthen the monitoring of harmful habits of youth in the use of online applications. In addition, the problem of youth addiction to short videos has become a major concern for education experts and the general public, thus demonstrating that short video addiction is indeed an ongoing research issue. Therefore, this study aimed to understand the causes of short-form video addiction and its impact on the psychology of learning, and to investigate the relationship between short-form video flow experience, short-form video addiction, intrinsic and extrinsic learning motivation, and learning well-being from the perspectives of flow experience theory and micro ecological systems. The questionnaire was sentviainstant messaging software such as QQ and WeChat, and university students from vocational colleges in China were invited to complete the questionnaire. A total of 517 valid data were collected, including 222 (42.9%) were male students and 295 (57.1%) were female students. The collected questionnaires were analyzed for reliability and validity after removing incomplete data, followed by structural equation modeling for model verification. The findings showed that: (1) short video flow experience had a positive effect on short video addiction; (2) short video addiction had a negative effect on intrinsic and extrinsic learning motivation; (3) intrinsic and extrinsic learning motivation had a positive effect on learning well-being; (4) short video flow experience had an indirect negative effect on intrinsic and extrinsic learning motivation; (5) short video flow and short video addiction had indirect negative effects on learning well-being. According to the results, it is clear that addiction to short videos has a negative impact on learners' learning motivation and positive psychology of learning, so parents and teachers should effectively guide students to use short video apps in a self-controlled way.
Burnout and Its Antecedents: Considering Both Work and Household Time Claims, and Flexibility in Relation to Burnout
Authors: Li Shuanglong , ten Berge Jannes , Kristiansen Marcus H.
Published: May 2022

This study contributes to the previous literature by examining how flexible work arrangements interact with work and family time claims to affect burnout. It does so by providing a theoretical framework and empirical test of the interaction of flexibility with the effect of work and family time claims on burnout. Hypotheses and predictions based on previous literature are tested by Ordinary Least Squared regression models using data from the Time Competition Survey, constituting a sample of 1,058 employees of 89 function groups within 30 organizations. We found no main effects of work and family time claims or flexible work arrangements on burnout. However, the results do show an interaction of flexible working hours with the effect of work and family time claims on burnout. Specifically, the higher an individual's work and family time claims, the more this person benefits from having flexible working hours. In general, the results support the proposition that the relationship between work and family time claims and burnout differs for individuals with different levels of flexible work arrangements.
Case Report: Disseminated Nocardiosis Caused by Nocardia vulneris in a Patient With Macroglobulinemia
Authors: Qiu Fulan , Ma Zhiyi , Zhong Rongrong , Huang Haonan , Wang Yuehua , Liu Hui
Published: May 2022

This report describes a case of disseminated nocardiosis, caused by Nocardia vulneris, in a 61-year-old man with macroglobulinemia and presenting with repeated fever, cough, shortness of breath, and muscle pain. The isolated Nocardia strain was resistant to ciprofloxacin, but susceptible to amikacin, gentamicin, tobramycin, linezolid, trimethoprim-sulfamethoxazole, amoxicillin/clavulanic, moxifloxacin, ceftriaxone, cefotaxim, and imipenem. The patient was started on combined meropenem and doxycycline treatment, followed by trimethoprim-sulfamethoxazole, which was subsequently switched to a combination treatment of linezolid, amikacin, and trimethoprim-sulfamethoxazole. The patient recovered, and his condition remained stable. Although infection by Nocardia vulneris is rare, and it is easy to miss detection in clinical practice, clinicians should be aware of the possibility of this infection. In addition, the MIC value of the drug sensitivity test should be ascertained when there is a wide choice of medicines. The current case was treated successfully with linezolid, amikacin, and trimethoprim-sulfamethoxazole. In cases of disseminated nocardiosis, the patient should be treated with antimicrobial therapy for at least 12 months. Furthermore, bacteriological examination and antimicrobial susceptibility testing should be performed regularly.
Lenvatinib Plus Pembrolizumab vs. Chemotherapy in Pretreated Patients With Advanced Endometrial Cancer: A Cost-Effectiveness Analysis
Authors: Feng Mingyang , Chen Yue , Yang Yang , Li Qiu
Published: May 2022

Background

In the international, randomized, open-label, phase 3 study 309-KEYNOTE-775 trial, lenvatinib plus pembrolizumab (LP) showed improved progression-free survival (PFS) and overall survival (OS) compared with chemotherapy in pretreated patients with advanced endometrial cancer. This study aimed to investigate whether LP is cost-effective compared with chemotherapy.

Materials and Methods

The clinical data for this model was derived from the 309-KEYNOTE-775 trial. Costs and utilities were either derived from the standard fee database or extracted from previously published literature. A three-state Markov model was developed to simulate the disease process of patients with advanced endometrial cancer. One-way sensitivity analyses were conducted to investigate the impact of variables in the analysis model. Probabilistic sensitivity analysis was performed based on 10,000 Monte-Carlo simulations. A subgroup analysis was performed to test whether LP is cost-effective in patients with mismatch repair–proficient (pMMR) disease.

Results

Lenvatinib plus pembrolizumab provided an incremental 0.64 quality-adjusted life years (QALYs) with an incremental cost of $241,278.18, compared with chemotherapy, resulting in the incremental cost-effectiveness ratio (ICER) of $378,251.44/QALY, which exceeded the willingness to pay (WTP) threshold. While in the pMMR subgroup, the ICER increased to $413,256.68/QALY. The variance of the utility of PFS state, the cost of LP, and the utility of the progressive disease state were the most influential factors in the sensitivity analysis.

Conclusion

Under the current WTP threshold, LP is not cost-effective compared with chemotherapy in pretreated patients with advanced endometrial cancer.
Artificial Intelligence and Employment: New Cross-Country Evidence
Authors: Georgieff Alexandre , Hyee Raphaela
Published: May 2022

Recent years have seen impressive advances in artificial intelligence (AI) and this has stoked renewed concern about the impact of technological progress on the labor market, including on worker displacement. This paper looks at the possible links between AI and employment in a cross-country context. It adapts the AI occupational impact measure developed by Felten, Raj and Seamans—an indicator measuring the degree to which occupations rely on abilities in which AI has made the most progress—and extends it to 23 OECD countries. Overall, there appears to be no clear relationship between AI exposure and employment growth. However, in occupations where computer use is high, greater exposure to AI is linked to higher employment growth. The paper also finds suggestive evidence of a negative relationship between AI exposure and growth in average hours worked among occupations where computer use is low. One possible explanation is that partial automation by AI increases productivity directly as well as by shifting the task composition of occupations toward higher value-added tasks. This increase in labor productivity and output counteracts the direct displacement effect of automation through AI for workers with good digital skills, who may find it easier to use AI effectively and shift to non-automatable, higher-value added tasks within their occupations. The opposite could be true for workers with poor digital skills, who may not be able to interact efficiently with AI and thus reap all potential benefits of the technology1.
Quintuple Helix Lens for Transformation: An Okayama Model of Education for Sustainable Development
Authors: Zen Irina Safitri , Shibakawa Hiroko
Published: May 2022

What transforms society? Using the quintuple helix model (QHM) of social innovation, this study examines how the Okayama Education for Sustainable Development (ESD) project has transformed the local community and its people, and how this has led to global recognition. Okayama is known as a world leader in ESD and their unique approach is called the Okayama Model of ESD. This study further looks at the institutional configuration on the elements contributed to knowledge co-creation and how the key actors interacted to contribute to societal transformation through knowledge, social innovation, and institutional setting. The goal of this study is to outline the Okayama Model of ESD using the QHM lens constituted of five helices; education, politics, society, economy, and the natural environment. This study applies a qualitative research method, in which key actors who contribute most to the development of the Okayama Model of ESD are identified by content analysis and semi-structured interviews that are conducted using the life history method. The result shows that the firm ground of the political subsystem facilitates the interaction among the stakeholders in the three subsystems–education, social, and natural environment, which ultimately contributes to the joining of the economic subsystem and the initiation of the knowledge circulation process. Transformation necessitates a city-wide approach involving a network of multiple actors to collaborate for knowledge co-creation and circulation, and the establishment of a new social values system. The study revealed several key points of local action that accelerated the transformation process by helping in value creation, knowledge convergence, and system interaction, which was instilled early through all forms of education—multiple actors' interaction that shapes through the ESD project that stimulates the triangulation of mind, hearts, and hands. This way, the city of Okayama functions as a living laboratory for the Okayama Model of ESD. This situation naturally promotes Mode 3 of the knowledge co-creation system, and the principles of civic collaboration and citizen engagement developed through the Okayama Model of ESD have been elaborated in the prefecture-wide vision statement.
Where Do Ocean Microbes With Nitrogen-Breathing Superpowers Live?
Authors: Zakem Emily J. , Lauderdale Jonathan M. , Follows Michael J.
Published: May 2022

All animals, including you, need oxygen to breathe. Many kinds of tiny microorganisms (microbes) also need oxygen. But some microbes have a superpower: they can breathe a different element called nitrogen! This means they can live in areas where there is no oxygen. But where are these areas? We wanted to figure out where these superpowered nitrogen-breathing microbes live in the ocean. In this article, we describe how we found a new way to estimate these areas. We made a map of the world that points out the areas in the deep ocean where nitrogen-breathing microbes are likely to live. Our work will help us to understand the role that nitrogen-breathing microbes play in the regulation of Earth’s climate.
Planetary Nebulae as Sources of Chemical Enrichment of the Galaxy
Authors: Kwok Sun
Published: May 2022

During the late asymptotic giant branch (AGB) phase of stellar evolution, the element carbon is created through the triple-α nuclear reaction. This is followed by the synthesis of other heavy elements via neutron capture in the core and the dredge-up of these elements to the surface by convection. Simple molecules and solid-state minerals begin to form in the upper photosphere. These molecules and solids are ejected into the interstellar medium by a strong stellar wind. During the subsequent post-AGB evolution, complex organics with aromatic and aliphatic structures are synthesized in the circumstellar envelope. Planetary nebulae, formed by swept-up circumstellar material, are found to show strong spectral signatures of ions, atoms, molecules, and inorganic and organic solids. These ejected materials of planetary nebulae serve as primordial ingredients of formation of new stars and planetary systems. Stellar synthesized organic solids may survive their journeys through the interstellar medium and evidence for remnants of such organics can be found in our own Solar System. In this paper, we summarize the recent observations of circumstellar synthesis of molecules and solids and discuss the implications of these ejected circumstellar materials on the chemical enrichment of the Galaxy and planetary systems.
Case Report: The Different Fates of Three Aneurysms: Diagnosis and Treatment Strategies for Unruptured Intracranial Aneurysms With Other Intracranial Diseases
Authors: Guo Gaochao , Zhao Liming , Wu Ruiyu , Xue Bingqian , Zhang Shao , Liang Hao , Gao Tao , Sun Yuxue , Liu Yang , Li Chaoyue
Published: May 2022

Intracranial aneurysms are vascular diseases characterized by local aneurysms of intracranial arteries. Their etiology involves a variety of environmental and genetic factors. Unruptured intracranial aneurysms (UIAs) are more common in intracranial aneurysms, but once an aneurysm is ruptured, the fatality rate and disability rate are extremely high. Therefore, accurate assessment of each step in the detection of intracranial aneurysms, assessment of the risk of rupture, formulation of treatment strategies, and treatment benefits will help to better treat the disease. At present, the treatment of intracranial aneurysms is limited. Except for surgical intervention, there are no other effective methods. Therefore, when a patient has a UIA, the formulation of treatment and management strategies is a difficult issue facing neurosurgery. This article introduces the choice of different treatment strategies for 3 patients with intracranial aneurysms complicated with other diseases and reviews the literature.
Application of Intraoperative Ultrasound Navigation in Neurosurgery
Authors: Simfukwe Keith , Iakimov Iurii , Sufianov Rinat , Borba Luís , Mastronardi Luciano , Shumadalova Alina
Published: May 2022

Effective intraoperative image navigation techniques are necessary in modern neurosurgery. In the last decade, intraoperative ultrasonography (iUS), a relatively inexpensive procedure, has gained widespread acceptance.

Aim

To document and describe the neurosurgery cases, in which iUS has been employed as the primary navigational tool. This includes a discussion of the advantages that iUS may possess relative to other forms of neuronavigation.

Conclusion

The application of iUS as an intraoperative navigation tool during neurosurgery holds great potential as it has been shown, relative to other neuronavigation techniques, to be quick, repeatable, and able to provide real-time results.
Identification of Key Genes Associated With Early Calf-Hood Nutrition in Subcutaneous and Visceral Adipose Tissues by Co-Expression Analysis
Authors: Pan Cuili , Yang Chaoyun , Ma Yanfen , Sheng Hui , Lei Zhaoxiong , Wang Shuzhe , Hu Honghong , Feng Xue , Zhang Junxing , Ma Yun
Published: May 2022

Background

Substantive evidence has confirmed that nutrition state is associated with health risk and the onset of pubertal and metabolic profile. Due to heterogeneity, adipose tissues in different anatomical positions tend to show various metabolic mechanisms for nutrition. To date, the complicated molecular mechanisms of early calf-hood nutrition on bovine adipose tissue are still largely unknown. This study aimed to identify key genes and functionally enriched pathways associated with early calf-hood nutrition in visceral and subcutaneous adipose tissue.

Results

The RNA-seq data of visceral and subcutaneous adipose tissues of calves feeding on low and high dietary nutrition for more than 100 days were downloaded and analyzed by weighted gene co-expression network analysis (WGCNA). Two modules that positively associated with a low plane of nutrition diet and two modules with a high plane of nutrition diet were identified in the subcutaneous adipose tissue. The blue and yellow modules, most closely associated with low and high nutrition, were selected for the functional enrichment analysis and exploration of hub genes. The results showed that genes in the blue module were significantly enriched in pathways that related to fat metabolism, reproduction, and cell communication. Genes in the yellow module were enriched in pathways related to fat metabolism, reproduction, cell proliferation, and senescence. Meanwhile, the blue and brown modules in visceral adipose tissue were most closely associated with low and high nutrition, respectively. Notably, genes of the blue module were significantly enriched in pathways related to substance metabolism, and genes in the brown module were significantly enriched in energy metabolism and disease pathways. Finally, key genes in subcutaneous adipose tissue for low nutrition (PLCG1, GNA11, and ANXA5) and high nutrition (BUB1B, ASPM, RRM2, PBK, NCAPG, and MKI67), and visceral adipose tissue for low nutrition (RPS5, RPL4, RPL14, and RPLP0) and high nutrition (SDHA and AKT1) were obtained and verified.

Conclusion

The study applied WGCNA to identify hub genes and functionally enriched pathways in subcutaneous and visceral adipose tissue and provided a basis for studying the effect of early calf-hood nutrition on the two adipose tissue types.
Why Do People Want Dogs? A Mixed-Methods Study of Motivations for Dog Acquisition in the United Kingdom
Authors: Holland Katrina E. , Mead Rebecca , Casey Rachel A. , Upjohn Melissa M. , Christley Robert M.
Published: May 2022

With an estimated 12. 5 million dogs in the UK alone, many people acquire a dog at some point during their lives. However, there are gaps in understanding about why UK owners decide to get dogs. Using a mixed-methods convergent design, this study identified the reasoning behind dog acquisition in a sample of UK current and prospective owners. An online survey of current (n = 8,050) and potential (n = 2,884) dog owners collected quantitative and qualitative data. Current owners were asked about the acquisition of their most recently acquired dog, whilst potential owners were asked about their dog ownership aspirations. Additional qualitative data were collected through semi-structured interviews with current (n = 166) and potential (n = 10) dog owners. Interviews focused on the factors that affected why and how people acquire dogs. Of survey responses, companionship for the respondent was the most common reason for wanting to get a dog, reported by 79.4 and 87.8% of current and potential owners, respectively. Facilitating exercise was reported as a reason for wanting to get a dog by 48.2 and 69.7% of current and potential owners, respectively. There were significant differences between current and potential owners in their likelihood of reporting pre-defined reasons, factors and influences involved in their decision to get a dog. Compared to current owners, potential owners were significantly more likely to report being motivated by most of the survey response options offered (including companionship for themselves or other adults in the household, helping a dog in need, lifestyle changes and previous experiences of meeting dogs), suggesting that current ownership status may affect experience and/or reporting expectations around dog ownership. Reflexive thematic analysis of qualitative data confirmed the importance of these motivations and identified additional reasons and factors that drive dog acquisition. These were organized into three overarching themes: Self-Related Motivation, Social-Based Motivation, and Dog-Related Positive Affect-Based Motivation. These findings provide insights into owners' expectations of ownership which may inform the development of interventions to support potential owners' decision-making around acquisition to maximize both dog and human welfare.
Panax Notoginseng Saponins Suppress Type 2 Porcine Reproductive and Respiratory Syndrome Virus Replication in vitro and Enhance the Immune Effect of the Live Vaccine JXA1-R in Piglets
Authors: Yi Heyou , Yu Zhiqing , Wang Qiumei , Sun Yankuo , Peng Jie , Cai Yu , Ma Jun , Chen Yongjie , Qin Chenxiao , Cai Mengkai , Ji Chihai , Zhang Guihong , Wang Heng
Published: May 2022

Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses the innate immune response in the host, reducing and delaying neutralizing antibody production against PRRSV infection and promoting viral infection. Here, we aimed to assess the potential of Panax notoginseng saponins (PNS) for improving the immune response exerted upon PRRSV-2-modified live virus (MLV) vaccine administration. Thirty piglets were randomly divided into six groups. Group 1 piglets were injected with medium 0 days post vaccination (dpv). Group 2 piglets were fed PNS 0–28 dpv. Group 3 and group 4 piglets were administered the JXA1-R vaccine 0 dpv. Group 4 piglets were also fed PNS 0–28 dpv. Group 1–4 piglets were challenged intranasally with the PRRSV JXA1 strain 28 dpv. Group 5 piglets were fed with PNS without challenge. Group 6 piglets served as controls. During the experiment, the samples were collected regularly for 49 days. Compared with group 1 piglets, group 3 piglets showed significantly reduced viremia and clinical scores, and significantly increased average daily gain (ADWG). Compared with group 3 piglets, group 4 piglets showed significantly improved neutralizing antibody titers, IFN-α and IFN-β mRNA expression, and significantly decreased viremia and viral load in the lungs and lymph nodes, but did not demonstrate any further improvement in PRRSV-specific antibody titer, rectal temperature, ADWG, or clinical scores. PNS upregulates neutralizing antibodies against PRRSV-2 and enhances the expression of IFN-α and IFN-β, which may reduce PRRSV viremia upon PRRSV-2 MLV vaccine administration. PNS may serve as an effective immunomodulator for boosting the immune defense against PRRSV.
SARS-CoV-2 Infection Can Lead to an Increase in Tacrolimus Levels in Renal Transplant Patients: A Cohort Study
Authors: Chalklin Christopher G. , Koimtzis Georgios , Khalid Usman , Carrington-Windo Eliot , Elker Doruk , Asderakis Argiris
Published: May 2022

The aim of this study is to evaluate the effect of SARS-CoV-2 infection on serum tacrolimus levels. Tacrolimus levels of 34 transplant patients diagnosed with SARS-CoV-2 in 2020 were compared with their pre-infection values and those of a control group with alternative infections. 20 out of 34 (59%) had high levels. At diagnosis, median tacrolimus level in the SARS-CoV-2 cohort was 9.6 μg/L (2.7–23) compared to 7.9 μg/L in the control group (p = 0.07, 95% CI for difference −0.3–5.8). The ratio of post-infection to pre-infection tacrolimus values was higher in the SARS-CoV-2 group (1.7) compared to the control group (1.25, p = 0.018, 95% CI for difference 0.08–0.89). The acute kidney injury rate was 65% (13 of 20) in SARS-CoV-2 patients with a level >8 μg/dl, compared to 29% (4 of 14) in those with lower levels (p = 0.037). Median length of stay was 10 days among SARS-CoV-2 infected patients with high tacrolimus levels compared to 0 days in the rest (p = 0.04). Four patients with high levels died compared to 2 in the control group. Clinicians should be aware of this potential effect on tacrolimus levels and take appropriate measures.
Post-Transplant Extracorporeal Membrane Oxygenation for Severe Primary Graft Dysfunction to Support the Use of Marginal Donor Hearts
Authors: Shudo Yasuhiro , Alassar Aiman , Wang Hanjay , Lingala Bharathi , He Hao , Zhu Yuanjia , Hiesinger William , MacArthur John W. , Boyd Jack H. , Lee Anson M. , Currie Maria , Woo Y. Joseph
Published: May 2022

Severe primary graft dysfunction (PGD) is the leading cause of early postoperative mortality following orthotopic heart transplantation (OHT). Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) has been used as salvage therapy. This study aimed to evaluate the outcomes in adult OHT recipients who underwent VA-ECMO for severe PGD. We retrospectively reviewed 899 adult (≥18 years) patients who underwent primary OHT at our institution between 1997 and 2017. Recipients treated with VA-ECMO (19, 2.1%) exhibited a higher incidence of previous cardiac surgery (p = .0220), chronic obstructive pulmonary disease (p = .0352), and treatment with a calcium channel blocker (p = .0018) and amiodarone (p = .0148). Cardiopulmonary bypass (p = .0410) and aortic cross-clamp times (p = .0477) were longer in the VA-ECMO cohort and they were more likely to have received postoperative transfusion (p = .0013); intra-aortic balloon pump (IABP, p < .0001), and reoperation for bleeding or tamponade (p < .0001). The 30-day, 1-year, and overall survival after transplantation of non-ECMO patients were 95.9, 88.8, and 67.4%, respectively, compared to 73.7, 57.9, and 47.4%, respectively in the ECMO cohort. Fourteen (73.7%) of the ECMO patients were weaned after a median of 7 days following OHT (range: 1–12 days). Following OHT, VA-ECMO may be a useful salvage therapy for severe PGD and can potentially support the usage of marginal donor hearts.
Epidemiological Study of Tricuspid Regurgitation After Cardiac Transplantation. Does it Influence Survival?
Authors: López-Vilella Raquel , Paniagua-Martín María J. , González-Vílchez Francisco , Donoso Trenado Víctor , Barge-Caballero Eduardo , Sánchez-Lázaro Ignacio , Aller Fernández Ana V. , Martínez-Dolz Luis , Crespo-Leiro María G. , Almenar-Bonet Luis
Published: May 2022

Background: Tricuspid valve disease is the most frequent valvulopathy after heart transplantation (HTx). Evidence for the negative effect of post-transplant tricuspid regurgitation (TR) on survival is contradictory. The aim of this study was to analyze the causes of post-transplant TR and its effect on overall mortality.

Methods: This is a retrospective observational study of all transplants performed in two Spanish centers (1009 patients) between 2000 and 2019. Of the total number of patients, 809 had no TR or mild TR and 200 had moderate or severe TR. The etiology of TR was analyzed in all cases.

Results: The prevalence of moderate and severe TR was 19.8%. The risk of mortality was greater when TR was caused by early primary graft failure (PGF) or rejection (p < 0.05). TR incidence was related to etiology: incidence of PGF-induced TR was higher in the first period, while TR due to rejection and undefined causes occurred more frequently in three periods: in the first year, in the 10–14-year period following HTx, and in the long term (16–18 years). In the multivariable analysis, TR was significantly associated with mortality/retransplantation (HR:1.04, 95% CI:1.01–1.07, p:0.02).

Conclusion: The development of TR after HTx is relatively frequent. The annual incidence depends on TR severity and etiology. The risk of mortality is greater in severe TR due to PGF or rejection.
Immune Response to Third Dose BNT162b2 COVID-19 Vaccine Among Kidney Transplant Recipients—A Prospective Study
Authors: Yahav Dafna , Rahamimov Ruth , Mashraki Tiki , Ben-Dor Naomi , Steinmetz Tali , Agur Timna , Zingerman Boris , Herman-Edelstein Michal , Lichtenberg Shelly , Ben-Zvi Haim , Bar-Haim Erez , Cohen Hila , Rotem Shahar , Elia Uri , Margalit Ili , Zvi Benaya Rozen
Published: May 2022

Immune response to two SARS-CoV-2 mRNA vaccine doses among kidney transplant recipients (KTRs) is limited. We aimed to evaluate humoral and cellular response to a third BNT162b2 dose. In this prospective study, 190 KTRs were evaluated before and ∼3 weeks after the third vaccine dose. The primary outcomes were anti-spike antibody level >4160 AU/ml (neutralization-associated cutoff) and any seropositivity. Univariate and multivariate analyses were conducted to identify variables associated with antibody response. T-cell response was evaluated in a subset of participants. Results were compared to a control group of 56 healthcare workers. Among KTRs, we found a seropositivity rate of 70% (133/190) after the third dose (37%, 70/190, after the second vaccine dose); and 27% (52/190) achieved levels above 4160 AU/ml after the third dose, compared to 93% of controls. Variables associated with antibody response included higher antibody levels after the second dose (odds ratio [OR] 30.8 per log AU/ml, 95% confidence interval [CI]11–86.4, p < 0.001); and discontinuation of antimetabolite prior to vaccination (OR 9.1,95% CI 1.8–46.5, p = 0.008). T-cell response was demonstrated in 13% (7/53). In conclusion, third dose BNT162b2 improved immune response among KTRs, however 30% still remained seronegative. Pre-vaccination temporary immunosuppression reduction improved antibody response.
Patient Experience in Pancreas-Kidney Transplantation—A Methodological Approach Towards Innovation in an Established Program
Authors: Ventura-Aguiar Pedro , Bayés-Genís Beatriu , Amor Antonio J. , Cuatrecasas Miriam , Diekmann Fritz , Esmatjes Enric , Ferrer-Fàbrega Joana , García-Criado Ángeles , Musquera Mireia , Olivella Silvia , Palou Eva , Paredes David , Perea Sonia , Perez Anna , Poch Esteban , Romano Barbara , Escarrabill Joan
Published: May 2022

Simultaneous pancreas-kidney transplantation (SPKT) leads to increased survival and quality of life, and is an alternative treatment for insulin-dependent diabetes mellitus and end-stage kidney disease. Due to the particularities of this population (often with multiple comorbidities) and of the surgery (only performed in a few centers), a comprehensive analysis of patients’ experience along the SPKT process is crucial to improve patient care and add value to this procedure. Therefore, we applied a systematic and iterative methodology with the participation of both patients and professional teams working together to explore and identify unmet needs and value-adding steps along the transplant patient journey at an established pancreas transplant program. Four main steps (to comprehend, to explore, to experiment and to assess) led to several interventions around three major areas: Administration and logistics, information and communication, and perceived quality of assistance. As a result, both displacements to the hospital for diagnostic purposes and the time delay involved in joining the patient waiting list for transplantation were reduced in parallel to the administrative procedures. In conclusion, the methodological implementation of key organizational changes has great impact on overall patient experience. Further quantitative analysis from the patient’s perspective will consolidate our program and may add new prototype service design components.
Impact of Size Matching Based on Donor-Recipient Height on Kidney Transplant Outcomes
Authors: Tandukar Srijan , Wu Christine , Hariharan Sundaram , Puttarajappa Chethan
Published: May 2022

Transplantation of kidneys from shorter donors into taller recipients may lead to suboptimal allograft survival. The effect of discrepancy in donor and recipient heights (ΔHeight) on long term transplant outcomes is not known. Adult patients ≥18 years undergoing living or deceased donor (LD or DD) kidney transplants alone from donors ≥18 years between 2000 and 2016 in the United States were included in this observational study. The cohort was divided into three groups based on ΔHeight of 5 inches as 1) Recipient < Donor (DD: 31,688, LD: 12,384), 2) Recipient = Donor (DD: 84,711, LD: 54,709), and 3) Recipient > Donor (DD: 21,741, LD: 18,753). Univariate analysis showed a higher risk of DCGL and mortality in both DD and LD (p < 0.001 for both). The absolute difference in graft and patient survival between the two extremes of ΔHeight was 5.7% and 5.7% for DD, and 0.4% and 1.4% for LD. On multivariate analysis, the HR of DCGL for Recipient < Donor and Recipient > Donor was 0.95 (p = 0.05) and 1.07 (p = 0.01) in DD and 0.98 (p = 0.55) and 1.14 (p < 0.001) in LD. Similarly, the corresponding HR of mortality were 0.97 (p = 0.07) and 1.07 (p = 0.003) for DD and 1.01 (p < 0.001) and 1.05 (p = 0.13) for LD. For DGF, the HR were 1.04 (p = 0.1) and 1.01 (p = 0.7) for DD and 1.07 (p = 0.45) and 0.89 (p = 0.13) for LD. Height mismatch between the donor and recipient influences kidney transplant outcomes.
Delayed Graft Function Under the Microscope: Surveillance Biopsies in Kidney Transplantation
Authors: Castro Filho João Batista Saldanha De , Pompeo Jeferson De Castro , Machado Rafael Berlezi , Gonçalves Luiz Felipe Santos , Bauer Andrea Carla , Manfro Roberto Ceratti
Published: May 2022

Delayed graft function (DGF) is a common complication of kidney transplantation and frequently leads to the necessity of surveillance biopsies. The purpose of this study is to describe the histological findings in surveillance biopsies of deceased donor kidney transplant recipients and evaluate the risk factors for graft outcomes. This is a monocentric, retrospective study including kidney transplant recipients that underwent a graft biopsy during the DGF period between January 2006 and July 2019. 356 biopsies were performed in 335 deceased donor transplant recipients. Biopsies were analyzed according to the Banff classification. The main histological findings were: acute tubular necrosis in 150 biopsies (42.1%), acute rejection in 96 biopsies (26.9%), and borderline findings in 91 biopsies (25.5%). In the multivariate analysis, recipient age (p = 0.028) and DGF duration (p = 0.005) were associated with rejection, antibody-induction with anti-thymocyte globulin (ATG) was protective (p = 0.001). The occurrence of rejection was associated with lower death-censored graft survival (log-rank; p = 0.009). Surveillance biopsies of kidney grafts experiencing DGF remain an essential tool for the care of kidney transplant recipients. The recipient’s age and duration of DGF are independent risk factors for acute rejection, while antibody-induction therapy with ATG is associated with protection from its occurrence.
Current Trends in Organ Preservation Solutions for Pancreas Transplantation: A Single-Center Retrospective Study
Authors: Ferrer-Fàbrega Joana , Folch-Puy Emma , Lozano Juan José , Ventura-Aguiar Pedro , Cárdenas Gabriel , Paredes David , García-Criado Ángeles , Bombí Josep Antoni , García-Pérez Rocío , López-Boado Miguel Ángel , Rull Ramón , Esmatjes Enric , Ricart Maria José , Diekmann Fritz , Fondevila Constantino , Fernández-Cruz Laureano , Fuster Josep , García-Valdecasas Juan Carlos
Published: May 2022

Due to the high vulnerability of the pancreas to ischemia-reperfusion injury, choices regarding preservation solution markedly affect pancreas transplant success. A retrospective single-center analysis of 380 pancreas transplants (2000–2019) was performed to correlate current preservation solutions with transplant outcomes. Early graft failure requiring transplantectomy within 30 days post-transplant occurred in 7.5% for University of Wisconsin (UW) group (n = 267), 10.8% of Celsior (CS) group (n = 83), 28.5% of Histidine-Tryptophan-Ketoglutarate (HTK) group (n = 7), and none for Institut Georges Lopez-1 (IGL-1) group (n = 23). The most common causes of technical failures in this cohort included abdominal hemorrhage (8.4%); graft pancreatitis (3.7%); fluid collections (2.6%); intestinal complications (6.6%); and vascular thrombosis (20.5%). Although IGL-1 solution provided lower surgical complication rates, no significant differences were found between studied groups. Nevertheless, HTK solution was associated with elevated pancreatitis rates. The best graft survival was achieved at 1 year using UW and IGL-1, and at 3 and 5 years using IGL-1 (p = 0.017). There were no significant differences in patient survival after a median follow-up of 118.4 months. In this setting therefore, IGL-1 solution appears promising for perfusion and organ preservation in clinical pancreas transplantation, compared to other commonly used solutions.
Living Donor Liver Transplantation vs. Split Liver Transplantation Using Left Lateral Segment Grafts in Pediatric Recipients: An Analysis of the UNOS Database
Authors: Dalzell Christina , Vargas Paola A. , Soltys Kyle , Dipaola Frank , Mazariegos George , Oberholzer Jose , Goldaracena Nicolas
Published: May 2022

Split and LDLT in pediatric patients have the potential to decrease wait times and waitlist mortality. Using UNOS-STAR data, we compared outcomes of pediatric patients undergoing LDLT and SLT using LLS grafts. The baseline characteristics and post-operative outcomes were compared between groups. Actuarial graft and patient survival were analyzed with Kaplan-Meier curves. Between 2010 and 2019, 911 pediatric LT were included in the analysis (LD graft group, n = 508, split graft group, n = 403). LD graft recipients spent more time on the waitlist vs. the split graft group (60 (22–138) days vs. 46 (16–108) days; p = 0.007). LD recipients had a lower rate of graft failure, found in 9.8% of patients compared with 14.6% in the split graft group (p = 0.02). HAT was the most common graft failure cause, with similar rates. Graft and patient survival at 1-, 3-, and 5-years was comparable between LDLT and SLT. In subgroup analyses, patients with biliary atresia, those ≤10 kg or ≤10 years old receiving an LD graft showed improved graft survival. In conclusion, LDLT is associated with a lower rate of graft failure in pediatric patients. The use of LLS regardless of the type of donor is a safe way to facilitate access to transplantation to pediatric patients with acceptable short and long-term outcomes.
Invigorating Care Farm Ecosystem Based on Public Service Innovation: Case of South Korea
Authors: Lee Hodong , Kim Boyoung
Published: May 2022

Recently, the importance of care farming has been emphasized worldwide for the purpose of public health and healing, and, in particular, discussions on innovative transformation and expansion of the care farm ecosystem have continued in terms of convergence of agriculture and welfare. This study aims to present influencing factors based on a hierarchical concept framework for revitalizing care farm ecosystem based on public service innovation. To this end, the AHP methodology was used. Through previous studies, 16 variables were derived within four categories: recognition, structure, leadership, process, and recognition of variables that affect the activation of the care farm ecosystem and conceptualized them through Delphi techniques. In addition, a survey was conducted on 28 stakeholders in care farming to derive the importance of each variable. As a result of the analysis, ‘reliability’ was derived as the most important factor, followed by factors such as human competence, vision, civic participation, and innovation awareness. Hence, it was confirmed that trust and communication between stakeholders are important to lead the innovative public service ecosystem of care farm, and above all, human competence is an important influencing factor.
Maneuvering Spacecraft Orbit Determination Using Polynomial Representation
Authors: Zhou Xingyu , Qin Tong , Meng Linzhi
Published: May 2022

This paper proposed a polynomial representation-based method for orbit determination (OD) of spacecraft with the unknown maneuver. Different from the conventional maneuvering OD approaches that rely on specific orbit dynamic equation, the proposed method needs no priori information of the unknown maneuvering model. The polynomials are used to represent the unknown maneuver. A transformation is made for the polynomials to improve the convergence and robustness. The Extended Kalman Filter (EKF) is used to process incoming observation data by compensating the unknown maneuver using the polynomials. The proposed method is successfully applicated into the OD problem of spacecraft with trigonometric maneuver. Numerical simulations show that the eighth-order polynomials are accurate enough to represent a trigonometric maneuver. Moreover, Monte Carlo simulations show that the position errors are smaller than 1 km, and the maneuver estimated errors are no more than 0.1 mm/s2 using the eighth-order polynomials. The proposed method is accurate and efficient, and has potential applications for tracking maneuvering space target.
Effects of Granule Size Ranges on Dazomet Degradation and Its Persistence with Different Environmental Factors
Authors: Ren Lirui , Li Wenjing , Li Qingjie , Zhang Daqi , Fang Wensheng , Li Yuan , Wang Qiuxia , Jin Xi , Yan Dongdong , Cao Aocheng
Published: May 2022

Pesticides are considered the most effective way to protect crops. However, irrational use has caused resources waste and environmental pollution. Dazomet (DZ) is a soil fumigant that has been used in many countries for decades, although it has caused occasional crop damage or insufficient control efficacy in some circumstances. In this study, the effects of DZ’s granule size and exposure to various environmental conditions on DZ degradation when used as a fumigant were demonstrated. The degradation rate of DZ was closely related to granule size. The half-life of larger DZ granules was longer than smaller granules with all studied environmental factors. The degradation rate decreased as the DZ usage (90–360 mg/kg) increased and different granule sizes showed the same variation trend. The half-life in each of the five granule size ranges tested decreased significantly as the temperature increased. DZ half-life decreased by 4.67–6.59 times as the temperature increased from 4 to 35 °C. Moreover, DZ usage and temperature affected the half-life of granules >400 and 300–400 μm in diameter significantly more than <100 μm granules. The half-life of all DZ granule sizes was reduced by 13.9–47.4% in alkaline compared to acidic conditions (pH from 9 to 5). Moreover, elevated temperatures could not only promote the production of methyl isothiocyanate (MITC) but accelerate its dissipation. The interactions between DZ granule size, dosage, temperature, and pH provide practical guidance on methods to improve DZ’s efficacy against pests and reduce the risk of phytotoxicity.
Winners and Losers of the CAP’s Rural Development Policy in Poland
Authors: Kiryluk-Dryjska Ewa , Więckowska Barbara , Smolińska Kinga
Published: May 2022

The purpose of this study was to identify territorial clusters of Polish municipalities whose rural residents demonstrated higher or lower interest in the four programs of the CAP’s rural development policy compared to the country’s average interest. The clusters were identified on an aggregate basis and described with synthetic indexes of agriculture and rural development. The Moran’s global spatial autocorrelation coefficient was used to check for spatial autocorrelation. The results demonstrated that the support offered under the rural development programs covered by this analysis was higher in the regions with well-developed agricultural structures, while the areas with structural defects and the areas in need of development had lower-than-average levels of applications for all programs (regardless of their objectives). These findings call for a clear strategy to change the unfavorable structures in Polish agriculture. Otherwise, the regional development gaps in Polish agriculture will only deepen under these programs.
Overexpression of CgbHLH001, a Positive Regulator to Adversity, Enhances the Photosynthetic Capacity of Maize Seedlings under Drought Stress
Authors: Zhao Haiju , Abulaizi Ailiman , Wang Changhai , Lan Haiyan
Published: May 2022

Drought is a major environmental factor limiting crop productivity. Photosynthesis is very sensitive to drought. Basic helix-loop-helix (bHLH) transcription factors (TFs) are important in response to abiotic stress. However, their functions remain unclear. Herein, we generated CgbHLH001 (a TF gene from halophyte Chenopodium glaucum)-overexpressed (OE) and ZmbHLH-RNA interference (Ri) maize lines to investigate their photosynthesis-associated indexes under drought conditions. The photosynthetic capacity was increased in OE lines under drought stress compared with that in non-transgenic (NT) and Ri plants. A greater root biomass, higher root/shoot ratio, and a relatively lower leaf area reduction ratio was also observed in OE plants. Compared to NT and Ri plants, OE lines showed a higher chlorophyll content and net photosynthetic rate and better chlorophyll fluorescence parameters under drought conditions. Fructose and glucose contents were also significantly elevated in OE lines. Moreover, under stressful conditions, CgbHLH001 overexpression increased the expression of genes related to photosynthesis. Transcriptomic data showed that many differentially expressed genes were enriched in the photosynthetic system in OE and Ri plants under drought conditions and were prone to being upregulated under drought stress in OE plants. Therefore, our results suggest that CgbHLH001 improves photosynthetic efficiency under drought stress and confers drought tolerance in maize seedlings.