bluuebunny/crossref_metadata_2025 huggingface dataset:
A High-Sugar Diet Consumption, Metabolism and Health Impacts with a Focus on the Development of Substance Use Disorder: A Narrative Review
Authors: Witek Kacper , Wydra Karolina , Filip Małgorzata
Published: July 2022

Carbohydrates are important macronutrients in human and rodent diet patterns that play a key role in crucial metabolic pathways and provide the necessary energy for proper body functioning. Sugar homeostasis and intake require complex hormonal and nervous control to proper body energy balance. Added sugar in processed food results in metabolic, cardiovascular, and nervous disorders. Epidemiological reports have shown enhanced consumption of sweet products in children and adults, especially in reproductive age and in pregnant women, which can lead to the susceptibility of offspring’s health to diseases in early life or in adulthood and proneness to mental disorders. In this review, we discuss the impacts of high-sugar diet (HSD) or sugar intake during the perinatal and/or postnatal periods on neural and behavioural disturbances as well as on the development of substance use disorder (SUD). Since several emotional behavioural disturbances are recognized as predictors of SUD, we also present how HSD enhances impulsive behaviour, stress, anxiety and depression. Apart from the influence of HSD on these mood disturbances, added sugar can render food addiction. Both food and addictive substances change the sensitivity of the brain rewarding neurotransmission signalling. The results of the collected studies could be important in assessing sugar intake, especially via maternal dietary patterns, from the clinical perspective of SUD prevention or pre-existing emotional disorders. Methodology: This narrative review focuses on the roles of a high-sugar diet (HSD) and added sugar in foods and on the impacts of glucose and fructose on the development of substance use disorder (SUD) and on the behavioural predictors of drugs abuse. The literature was reviewed by two authors independently according to the topic of the review. We searched the PubMed and Scopus databases and Multidisciplinary Digital Publishing Institute open access scientific journals using the following keyword search strategy depending on the theme of the chapter: “high-sugar diet” OR “high-carbohydrate diet” OR “sugar” OR “glucose” OR “fructose” OR “added sugar” AND keywords. We excluded inaccessible or pay-walled articles, abstracts, conference papers, editorials, letters, commentary, and short notes. Reviews, experimental studies, and epidemiological data, published since 1990s, were searched and collected depending on the chapter structure. After the search, all duplicates are thrown out and full texts were read, and findings were rescreened. After the selection process, appropriate papers were included to present in this review.
Hepatic Steatosis in Patients with Celiac Disease: The Role of Packaged Gluten-Free Foods
Authors: Raiteri Alberto , Granito Alessandro , Faggiano Chiara , Giamperoli Alice , Catenaro Teresa , Negrini Giulia , Tovoli Francesco
Published: July 2022

Background: An increased risk of nonalcoholic fatty liver disease (NAFLD) in patients with celiac disease (CD) adhering to a gluten-free diet (GFD) was recently reported. The nutritional composition of packaged gluten-free foods (PGFF) has been proposed as a possible cause. This hypothesis has not been investigated further, since a systematic structural nutritional interview for all patients would be problematic in clinical practice. Methods: We administered a simple questionnaire based on a Recency, Frequency, and Monetary value (RFM) analysis (a cornerstone of direct marketing segmentation) to consecutive CD patients on a GFD for >6 months and verified its association with NAFLD. Subgroup analyses were performed to understand whether specific patterns of PGFF consumption were significantly associated with NAFLD. Results: Amongst 147 patients (female 82%, median age 42 years), 45 (30.6%) had NAFLD. Total RFM score (adjusted odds ratio = 1.223, 95% CI: 1.059–1.413, p = 0.006), body mass index, and total cholesterol and triglycerides were independently related to NAFLD, and “Bread and bakery” (p = 0.002), “salty convenience” (p = 0.005), and “sweet convenience” (p = 0.049) products were significantly related with NAFLD. Also, questions about the number of purchased PGFF in the last month (monetary value) and different categories of PGFF consumed in the last week (recency) were particularly able to identify NAFLD patients. Conclusions: The specific GFD dietary habits of CD patients were correlated with the degree of risk of NAFLD. Information was obtained through a questionnaire which could be used in clinical practice to favor a patient-tailored approach and in future studies to verify the reproducibility of our results in different geographical areas.
Perception of Portuguese Consumers Regarding Food Labeling
Authors: Silva Bruna , Lima João P. M. , Baltazar Ana Lúcia , Pinto Ezequiel , Fialho Sónia
Published: July 2022

Food labels are the first information tool used by consumers in the purchase and consumption of food products. Food labeling is a tool that can influence the consumers’ perception of quality and, in turn, their food choice. This study characterizes and demonstrates the importance of labeling and the degree of consumers’ perception and literacy about food labels through the application of an online questionnaire. The results obtained, in a sample of n = 467, showed that Portuguese consumers have the habit of reading the label and recognizing its importance but do not understand all the information contained in the label. They have an easier time understanding the front-of-pack labeling systems, especially those presented through symbols/colors. Thus, it demonstrates the need for greater education and literacy in the areas of food and nutrition so that through the reading and interpretation of labels, consumers can make informed food choices.
Improvement of Depressed Mood with Green Tea Intake
Authors: Unno Keiko , Furushima Daisuke , Tanaka Yuya , Tominaga Takeichiro , Nakamura Hirotomo , Yamada Hiroshi , Taguchi Kyoko , Goda Toshinao , Nakamura Yoriyuki
Published: July 2022

Being in a prolonged depressed state increases the risk of developing depression. To investigate whether green tea intake is effective in improving depression-like moods, we used an experimental animal model of depression with lipopolysaccharide (LPS) and clarified the effects of green tea on the biological stress response and inflammation in the brain. Regarding the stress reduction effect of green tea, we found that the sum of caffeine (C) and epigallocatechin gallate (E) relative to the sum of theanine (T) and arginine (A), the major components of green tea, or the CE/TA ratio, is important. The results showed that depression-like behavior, adrenal hypertrophy as a typical stress response, and brain inflammation were suppressed in mice fed green tea components with CE/TA ratios of 2 to 8. In addition, the expression of Npas4, which is reduced in anxiety and depression, was maintained at the same level as controls in mice that consumed green tea with a CE/TA ratio of 4. In clinical human trials, the consumption of green tea with CE/TA ratios of 3.9 and 4.7 reduced susceptibility to subjective depression. These results suggest that the daily consumption of green tea with a CE/TA ratio of 4–5 is beneficial to improving depressed mood.
Assessing the Immunomodulatory Effect of Size on the Uptake and Immunogenicity of Influenza- and Hepatitis B Subunit Vaccines In Vitro
Authors: Heida Rick , Born Philip A. , Tapia-Calle Gabriela , Frijlink Henderik W. , Salvati Anna , Huckriede Anke L. W. , Hinrichs Wouter L. J.
Published: July 2022

Viral subunit vaccines are a safer and more tolerable alternative to whole inactivated virus vaccines. However, they often come with limited efficacy, necessitating the use of adjuvants. Using free and particle-bound viral antigens, we assessed whether size affects the uptake of those antigens by human monocyte-derived dendritic cells (Mo-DCs) and whether differences in uptake affect their capacity to stimulate cytokine production by T cells. To this end, influenza antigens and hepatitis B surface antigen (HBsAg) were covalently conjugated to polystyrene particles of 500 nm and 3 μm. Cellular uptake of the antigens, either unconjugated or conjugated, and their capacity to stimulate T cells within a population of human peripheral blood mononuclear cells (PBMCs) were measured by flow cytometry. Conjugation of both antigens to particles significantly increased their uptake by Mo-DCs. Moreover, both the 500 nm and 3 μm influenza conjugates induced significantly higher numbers of cytokine-producing CD4+ T cells and induced increased production of the pro-inflammatory cytokines IFNγ and TNFα. In contrast, conjugation of HBsAg to particles did not notably affect the T cell response. In conclusion, conjugation of antigen to 500 nm and 3 μm particles leads to increased antigen uptake by human Mo-DCs, although the capacity of such conjugates to induce T cell stimulation likely depends on the immunological status of the PBMC donor.
Application of Plackett–Burman Design for Spectrochemical Determination of the Last-Resort Antibiotic, Tigecycline, in Pure Form and in Pharmaceuticals: Investigation of Thermodynamics and Kinetics
Authors: El-Shafie Ahmed S. , Yousef Aseel , El-Azazy Marwa
Published: July 2022

Tigecycline (TIGC) reacts with 7,7,8,8-tetracyanoquinodimethane (TCNQ) to form a bright green charge transfer complex (CTC). The spectrum of the CTC showed multiple charge transfer bands with a major peak at 843 nm. The Plackett–Burman design (PBD) was used to investigate the process variables with the objective being set to obtaining the maximum absorbance and thus sensitivity. Four variables, three of which were numerical (temperature—Temp; reagent volume—RV; reaction time—RT) and one non-numerical (diluting solvent—DS), were studied. The maximum absorbance was achieved using a factorial blend of Temp: 25 °C, RV: 0.50 mL, RT: 60 min, and acetonitrile (ACN) as a DS. The molecular composition that was investigated using Job’s method showed a 1:1 CTC. The method’s validation was performed following the International Conference of Harmonization (ICH) guidelines. The linearity was achieved over a range of 0.5–10 µg mL−1 with the limits of detection (LOD) and quantification (LOQ) of 166 and 504 ng mL−1, respectively. The method was applicable to TIGC per se and in formulations without interferences from common additives. The application of the Benesi–Hildebrand equation revealed the formation of a stable complex with a standard Gibbs free energy change (∆G°) value of −26.42 to −27.95 kJ/mol. A study of the reaction kinetics revealed that the CTC formation could be best described using a pseudo-first-order reaction.
Synthesis and Evaluation of Some New 4H-Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations
Authors: El-Sayed Nahed N. E. , Zaki Magdi E. A. , Al-Hussain Sami A. , Ben Bacha Abir , Berredjem Malika , Masand Vijay H. , Almarhoon Zainab M. , Omar Hanaa S.
Published: July 2022

Colorectal cancer oncogenesis is linked to dysbiosis, oxidative stress and overexpression of CDK2. The 4H-pyran scaffold is considered an antitumoral, antibacterial and antioxidant lead as well as a CDK2 inhibitor. Herein, certain 4H-pyran derivatives were evaluated as antibacterial, antioxidant and cytotoxic agents against HCT-116 cells. Derivatives 4g and 4j inhibited all the tested Gram-positive isolates, except for B. cereus (ATCC 14579), with lower IC50 values (µM) than ampicillin. In addition, 4g and 4j demonstrated the strongest DPPH scavenging and reducing potencies, with 4j being more efficient than BHT. In cell viability assays, 4d and 4k suppressed the proliferation of HCT-116 cells, with the lowest IC50 values being 75.1 and 85.88 µM, respectively. The results of molecular docking simulations of 4d and 4k, inhibitory kinase assays against CDK2, along with determination of CDK2 protein concentration and the expression level of CDK2 gene in the lysates of HCT-116 treated cells, suggested that these analogues blocked the proliferation of HCT-116 cells by inhibiting kinase activity and downregulating expression levels of CDK2 protein and gene. Moreover, 4d and 4k were found to induce apoptosis in HCT-116 cells via activation of the caspase-3 gene. Lastly, compounds 4g, 4j, 4d and 4k were predicted to comply with Lipinski’s rule of five, and they are expected to possess excellent physiochemical and pharmacokinetic properties suitable for in vivo bioavailability, as predicted by the SwissADME web tool.
PD-L1-Targeted Co-Delivery of Two Chemotherapeutics for Efficient Suppression of Skin Cancer Growth
Authors: Movahedi Fatemeh , Liu Jie , Sun Bing , Cao Pei , Sun Luyao , Howard Christopher , Gu Wenyi , Xu Zhi Ping
Published: July 2022

To overcome the severe side effects of cancer chemotherapy, it is vital to develop targeting chemotherapeutic delivery systems with the potent inhibition of tumour growth, angiogenesis, invasion and migration at low drug dosages. For this purpose, we co-loaded a conventional antiworm drug, albendazole (ABZ), and a TOPK inhibitor, OTS964, into lipid-coated calcium phosphate (LCP) nanoparticles for skin cancer treatment. OTS- and ABZ-loaded LCP (OTS-ABZ-LCP) showed a synergistic cytotoxicity against skin cancer cells through their specific cancerous pathways, without obvious toxicity to healthy cell lines. Moreover, dual-targeting the programmed death ligand-1 (PD-L1) and folate receptor overexpressed on the surface of skin cancer cells completely suppressed the skin tumour growth at low doses of ABZ and OTS. In summary, ABZ and OTS co-loaded dual-targeting LCP NPs represent a promising platform with high potentials against complicated cancers where PD-L1/FA dual targeting appears as an effective approach for efficient and selective cancer therapy.
Urea-Based Patches with Controlled Release for Potential Atopic Dermatitis Treatment
Authors: Krysiak Zuzanna J. , Stachewicz Urszula
Published: July 2022

Skin diseases such as atopic dermatitis (AD) are widespread and affect people all over the world. Current treatments for dry and itchy skin are mostly focused on pharmaceutical solutions, while supportive therapies such as ointments bring immediate relief. Electrospun membranes are commonly used as a drug delivery system, as they have a high surface to volume area, resulting in high loading capacity. Within this study we present the manufacturing strategies of skin patches using polymer membranes with active substances for treating various skin problems. Here, we manufactured the skin patches using electrospun poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB) fibers blended and electrosprayed with urea. The highest cumulative release of urea was obtained from the PVB patches manufactured via blend electrospinning with 5% of the urea incorporated in the fiber. The maximum concentration of released urea was acquired after 30 min, which was followed up by 6 h of constant release level. The simultaneous electrospinning and electrospraying limited the urea deposition and resulted in the lowest urea incorporation followed by the low release level. The urea-based patches, manufactured via blend electrospinning, exhibited a great potential as overnight treatment for various skin problems and their development can bring new trends to the textile-based therapies for AD.
The Role of the Excluded
Authors: Minati Gianfranco
Published: July 2022

We consider the peculiarity of unique events, such as those of a natural, evolutionary, and social nature. In particular, we consider unique social events that have had either the claim or the vocation of being salvific for humanity, such as the introduction over time of the Torah, Hinduism, Buddhism, Christianity, and Islam. We question how the claimed, general salvific vocation contrasts, or is inconsistent with, the non-retroactive temporality and locality of such events, which could not have happened otherwise. This undeclared and philosophically unsolved inconsistency then reappears in subsequent cultural contradictions and inadequacies, political and social allowances such as, for instance, homo-centrism and a pathological relation with Nature. In the case of Christianity, this inconsistency is represented by the painting reproduced in the article, a work in which the excluded humans and other living beings are represented as astonished by the occurrence in this moment, and in such an unnatural context. Furthermore, we consider the original understanding as related to concepts of classical physics, or of such concepts naively adopted within the texts considered sacred. However, in some religions, such as Christianity, the inconsistency is theologically solved. We stress the need to update the ancient original elementary, naïve, pre-classic philosophical and conceptual frameworks used so that these alleged inconsistencies and contradictions may be not only theologically solved, but also conceptually solved in more complex understandings of the world, for example, considering relativistic time, long-range interdependence, quantum entanglement, and theories of the universe. Without this update, the unique saving events can affect only religiously, that is, optionally, on the scientific and philosophical conceptions used. Without this adjustment, homo-centrist illusion and egoism prevail as the natural, linear consequential attitude without raising these questions. It rather assumes that the intervention is for involved human beings, and moreover for those who have had and are lucky enough to receive and practice it, ignoring the enormous inconsistency within the message itself, and its presumed general and available salvific nature. This requires theological, philosophical, and scientific interdisciplinarity. The theme concerns inconsistencies within and superficiality of the narratives and their treatment of the unique, salvific events, without any reference to possible general and retroactive effects of how these events are represented in the painting. We conclude that the subject should be debated by taking into account contemporary understandings, such as relativistic space and time, quantum physics, and of the universe, with new philosophical and anthropological approaches. This should be a matter of responsible philosophical and theological interdisciplinary debate involving science, suitable to establish new understandings.
Exogenously Applied GA3 Enhances Morphological Parameters of Tolerant and Sensitive Cyclamen persicum Genotypes under Ambient Temperature and Heat Stress Conditions
Authors: Cornea-Cipcigan Mihaiela , Cordea Mirela Irina , Mărgăoan Rodica , Pamfil Doru
Published: July 2022

Cyclamen genus is part of the Primulaceae family consisting of 24 species widely cultivated as ornamental and medicinal plants. They also possess high plasticity in terms of adaptability to alternating environmental conditions. In this regard, the present study investigates the germination and morphological parameters of heat-tolerant and heat-sensitive Cyclamen persicum accessions in the presence of different GA3 solutions (0, 30, 70 and 90 mg/L) under ambient temperature and heat stress conditions. Heat-tolerant genotypes, mainly C3-Smartiz Victoria (6.42%), C15-Merengue magenta (6.47%) and C16-Metis silverleaf (5.12%) had the highest germination rate with 90 mg/L GA3 treatment compared with control. Regarding heat-sensitive genotypes, C11-Verano (5.11%) and C13-Metis Origami (4.28%) had the lowest values in mean germination time, along with the Petticoat genotypes C1 (73.3%) and C2 (80.0%) with a high germination percentage. Heat-tolerant genotypes positively responded to GA3 (70 and 90 mg/L) even under heat stress conditions, by their higher values in plant height, an ascending trend also seen in heat-sensitive genotypes under GA3 treatment (70 and 90 mg/L). According to the hierarchical clustering, several heat-tolerant genotypes showed peculiar behavior under heat stress conditions, namely C3 (Smartiz Victoria), C7 (Halios falbala) and C8 (Latinia pipoca) which proved to be susceptible to heat stress even under GA3 application, compared with the other genotypes which showed tolerance to higher temperatures. In the case of heat-sensitive genotypes, C4 (Smartiz violet fonce), C6 (Metis blank pur), C11 (Verano) and C13 (Metis origami) possessed higher positive or negative values compared with the other heat-sensitive genotypes with increased doses of GA3. These genotypes were shown to be less affected by heat stress, suggesting their positive response to hormone treatment. In conclusion, the above-mentioned genotypes, particularly heat-tolerant C15 and heat-sensitive C2 with the highest germination capacity and development can be selected as heat-resistant genotypes to be deposited in gene banks and used in further amelioration programs under biotic and/or abiotic stresses to develop resistant genotypes.
Gene Losses and Plastome Degradation in the Hemiparasitic Species Plicosepalus acaciae and Plicosepalus curviflorus: Comparative Analyses and Phylogenetic Relationships among Santalales Members
Authors: AL-Juhani Widad , Al Thagafi Noha T. , Al-Qthanin Rahmah N.
Published: July 2022

The Plicosepalus genus includes hemiparasitic mistletoe and belongs to the Loranthaceae family, and it has several medicinal uses. In the present study, we sequenced the complete plastomes of two species, Plicosepalus acaciae and Plicosepalus curviflorus, and compared them with the plastomes of photosynthetic species (hemiparasites) and nonphotosynthetic species (holoparasites) in the order Santalales. The complete chloroplast genomes of P. acaciae and P. curviflorus are circular molecules with lengths of 120,181 bp and 121,086 bp, respectively, containing 106 and 108 genes and 63 protein-coding genes, including 25 tRNA and 4 rRNA genes for each species. We observed a reduction in the genome size of P. acaciae and P. curviflorus and the loss of certain genes, although this reduction was less than that in the hemiparasite and holoparasitic cp genomes of the Santalales order. Phylogenetic analysis supported the taxonomic state of P. acaciae and P. curviflorus as members of the family Loranthaceae and tribe Lorantheae; however, the taxonomic status of certain tribes of Loranthaceae must be reconsidered and the species that belong to it must be verified. Furthermore, available chloroplast genome data of parasitic plants could help to strengthen efforts in weed management and encourage biotechnology research to improve host resistance.
Role of Poly(ADP-Ribose) Polymerase (PARP) Enzyme in the Systemic Acquired Acclimation Induced by Light Stress in Phaseolus vulgaris L. Plants
Authors: Vitale Luca , Vitale Ermenegilda , Bianchi Anna Rita , De Maio Anna , Arena Carmen
Published: July 2022

Plants are able to acclimate to environmental constraints through functional modifications that may also occur in tissues that are not directly exposed to stress. This process is termed “systemic acquired acclimation.” The present study aims to evaluate the involvement of PolyADP-ribose) polymerase (PARP) protein in the acclimation process to high light (HL) stress in Phaseolus vulgaris plants. For this purpose, some leaves located at the top of the plant, in the apical position, were directly exposed to HL (“inducing” leaves), while others on the same plant, distal from the top, continued to be exposed to growth light (“receiving” leaves) to verify the hypothesis that an “alert” message may be transferred from injured tissues to distal ones. Biochemical and eco-physiological analyses, namely PARP activity, H2O2 and water- and fat-soluble antioxidants (i.e., ascorbic acid, tocopherol, glutathione (GSH), phenols, carotenoids, etc.) content, and chlorophyll fluorescence measurements were performed on both “inducing” and “receiving” leaves. Even if no change in PARP expression was found, its activity increased in “receiving” unstressed leaves in response to the light stress duration experimented by “inducing” leaves, while antioxidant capacity declined. When the “receiving” leaves were exposed to HL, the PARP activity returned to the control value, while antioxidant capacity photosynthetic electron transport rate (Jf) decreased and increased, respectively, compared to Control. Our results seem to show an acclimation pathway triggered in remote tissues not yet subjected to stress, likely involving a reactive oxygen species wave activating the PARP enzyme in a mechanism still to be clarified. In addition, the increased tolerance of plants directly exposed to HL could implicate a boosted synthesis of soluble antioxidants accompanied by a reduction of PARP activity to reduce excessive consumption of NAD(P).
Rhizospheric Actinomycetes Revealed Antifungal and Plant-Growth-Promoting Activities under Controlled Environment
Authors: Elshafie Hazem S. , Camele Ippolito
Published: July 2022

Actinomycetes has large habitats and can be isolated from terrestrial soil, rhizospheres of plant roots, and marine sediments. Actinomycetes produce several bioactive secondary metabolites with antibacterial, antifungal, and antiviral properties. In this study, some Actinomycetes strains were isolated from the rhizosphere zone of four different plant species: rosemary, acacia, strawberry, and olive. The antagonistic activity of all isolates was screened in vitro against Escherichia coli and Bacillus megaterium. Isolates with the strongest bioactivity potential were selected and molecularly identified as Streptomyces sp., Streptomyces atratus, and Arthrobacter humicola. The growth-promoting activity of the selected Actinomycetes isolates was in vivo evaluated on tomato plants and for disease control against Sclerotinia sclerotiorum. The results demonstrated that all bacterized plants with the studied Actinomycetes isolates were able to promote the tomato seedlings’ growth, showing high values of ecophysiological parameters. In particular, the bacterized seedlings with Streptomyces sp. and A. humicola showed low disease incidence of S. sclerotiorum infection (0.3% and 0.2%, respectively), whereas those bacterized with S. atratus showed a moderate disease incidence (7.6%) compared with the positive control (36.8%). In addition, the ability of the studied Actinomycetes to produce extracellular hydrolytic enzymes was verified. The results showed that A. humicola was able to produce chitinase, glucanase, and protease, whereas Streptomyces sp. and S. atratus produced amylase and pectinase at high and moderate levels, respectively. This study highlights the value of the studied isolates in providing bioactive metabolites and extracellular hydrolytic enzymes, indicating their potential application as fungal-biocontrol agents.
Ecological Niche Modeling of Water Lily (Nymphaea L.) Species in Australia under Climate Change to Ascertain Habitat Suitability for Conservation Measures
Authors: Nzei John M. , Mwanzia Virginia M. , Ngarega Boniface K. , Musili Paul M. , Wang Qing-Feng , Chen Jin-Ming , Li Zhi-Zhong
Published: July 2022

The International Panel on Climate Change (IPCC) projects a global temperature rise of 4.3 ± 0.7 °C by 2100 and an extinction of 8.5% in one out of every six species. Australia’s aquatic ecosystem is no exception; habitat loss, fragmentation, and loss of biodiversity are being experienced. As the center for Nymphaea species distribution, it presents the culturally, ecologically, and scientifically important genus as the best candidate for habitat suitability assessment in climate change, whose habitat suitability is presumed to decline. The models were run according to the maximum entropy (MaxEnt) method, using one general circulation model (GCM). Projections were made for the current, past, and future in medium (4.5) and high (8.5) representative concentration pathways. Significantly, bio2 and bio15 were highly preferred among the species. Less distribution was noted in West Australia compared to the north, east, and south of the continent, while north of the continent in Western Australia, Northern Territory, and Queensland indicate more habitat contractions compared to the east and southeast of Queensland and New South Wales, although it receives high precipitation. Generally, the species respond variably to both temperature and precipitation variables which is a key species response factor for planners and decision makers in species habitat and biodiversity conservation.
Performance for Fly Ash Reinforced HDPE Composites over the Ageing of Material Components
Authors: Alghamdi Mohammed N.
Published: July 2022

The by-product abundances of fly ash allow them to be used as the reinforcing filler for high-volume and high-performance thermoplastic composites. However, the durability of the composites remains questioned as polymer degradation during environmental weathering creates brittle materials, leading to surface cracks, which potentially release hazardous fly ash particles into the environment. This paper reports the effect of environmental ageing (UV and moisture exposure) on the morphological and mechanical properties of fly ash mixed high-density polyethylene (FA/HDPE) composites with three dissimilar weight fractions (5, 10 and 15 wt%) of filler and compared the results with similarly aged neat HDPE samples. The consequence of environmental ageing on the elevated mechanical properties of composites is investigated. Fifteen wt% fly ash reinforced composite appears to have better morphological and mechanical properties after 20 weeks of ageing, with only ~5 and ~9% reduction in Young’s modulus and tensile strength, respectively. The driving factors controlling the ageing effects are broadly discussed and recommendations are made for research advancements.
Novel Magnetic Elastic Phase-Change Nanodroplets as Dual Mode Contrast Agent for Ultrasound and Magnetic Resonance Imaging
Authors: Riaz Ramish , Waqar Hira , Ahmad Nasir M. , Abbas Shah Rukh
Published: July 2022

Recently, dual-mode imaging systems merging magnetic resonance imaging (MRI) and ultrasound (US) have been developed. Designing a dual-mode contrast agent is complex due to different mechanisms of enhancement. Herein, we describe novel phase change nanodroplets (PCNDs) with perfluoropentane encapsulated in a pre-polyglycerol sebacate (pre-PGS) shell loaded with polyethylene glycol (PEG)-coated iron oxide nanoparticles as having a dual-mode contrast agent effect. Iron oxide nanoparticles were prepared via the chemical co-precipitation method and PCNDs were prepared via the solvent displacement technique. PCNDs showed excellent enhancement in the in vitro US much more than Sonovue® microbubbles. Furthermore, they caused a susceptibility effect resulting in a reduction of signal intensity on MRI. An increase in the concentration of nanoparticles caused an increase in the MR contrast effect but a reduction in US intensity. The concentration of nanoparticles in a shell of PCNDs was optimized to obtain a dual-mode contrast effect. Biocompatibility, hemocompatibility, and immunogenicity assays showed that PCNDs were safe and non-immunogenic. Another finding was the dual-mode potential of unloaded PCNDs as T1 MR and US contrast agents. Results suggest the excellent potential of these PCNDs for use as dual-mode contrast agents for both MRI and US.
Polyimide Nanodielectrics Doped with Ultralow Content of MgO Nanoparticles for High-Temperature Energy Storage
Authors: Li Ziwei , Qin Hongmei , Song Jinhui , Liu Man , Zhang Xiaolin , Wang Shan , Xiong Chuanxi
Published: July 2022

Advanced polymer dielectrics with high energy density at elevated temperatures are highly desired to meet the requirements of modern electronic and electrical systems under harsh conditions. Herein, we report a novel polyimide/magnesium oxide (PI/MgO) nanodielectric that exhibits high energy storage density (Ue) and charge–discharge efficiency (η) along with excellent cycling stability at elevated temperatures. Benefiting from the large bandgap of MgO and the extended interchain spacing of PI, the composite films can simultaneously achieve high dielectric constant and high breakdown strength, leading to enhanced energy storage density. The nanocomposite film doped with 0.1 vol% MgO can achieve a maximum Ue of 2.6 J cm−3 and a η of 89% at 450 MV m−1 and 150 °C, which is three times that of the PI film under the same conditions. In addition, embedding ultralow content of inorganic fillers can avoid aggregation and facilitate its large-scale production. This work may provide a new paradigm for exploring polymer nanocomposites with excellent energy storage performance at high temperatures and under a high electric field.
Thermally Activable Bistetrazoles for Elastomers Crosslinking
Authors: Monti Mauro , Giannini Luca , Tadiello Luciano , Guerra Silvia , Papagni Antonio , Vaghi Luca
Published: July 2022

Sulfur vulcanization is the most used method for curing of natural and synthetic rubbers. The crosslinking degree achieved is usually controlled by adding proper quantities of accelerants, activators, co-activators, retardants, and inhibitors, and influences the hardness, elasticity, hysteresis of elastomers and, consequently, the properties and behavior of the materials that incorporate them. Despite the fine tuning pursued over the years, sulfur crosslinking is still difficult to control both in terms of degree and homogeneity of cross-link. Addition of thermally activable bifunctional reagents able to crosslink the polymer matrix through covalent bonds could be a strategy to modulate and control finely the reticulation grade of elastomers. Tetrazoles can form highly reactive nitrilimines by thermal treatment at appropriate temperature, which can react with the vinyl double bonds present in the rubber. In this work a set of bis-tetrazoles were synthesized and those with the right activation temperatures were used for the curing of styrene-butadiene rubber, acting both as single crosslinkers and together with classic sulfur-based ones. The addition of bistetrazoles simplified and made more efficient the compounding process, allowing to prolong the mixing until optimum dispersion and homogeneity were obtained. Moreover, they led to an improvement in the hysteretic properties of the compound and to the reduction of the non-linearity of the dynamic behavior (Payne effect).
In Silico Analysis Using SARS-CoV-2 Main Protease and a Set of Phytocompounds to Accelerate the Development of Therapeutic Components against COVID-19
Authors: Mustafa Sabeena , Alomair Lamya A. , Hussein Mohamed
Published: July 2022

SARS-CoV-2, the virus that caused the widespread COVID-19 pandemic, is homologous to SARS-CoV. It would be ideal to develop antivirals effective against SARS-CoV-2. In this study, we chose one therapeutic target known as the main protease (Mpro) of SARS-CoV-2. A crystal structure (Id: 6LU7) from the protein data bank (PDB) was used to accomplish the screening and docking studies. A set of phytocompounds was used for the docking investigation. The nature of the interaction and the interacting residues indicated the molecular properties that are essential for significant affinity. Six compounds were selected, based on the docking as well as the MM-GBSA score. Pentagalloylglucose, Shephagenin, Isoacteoside, Isoquercitrin, Kappa-Carrageenan, and Dolabellin are the six compounds with the lowest binding energies (−12 to −8 kcal/mol) and show significant interactions with the target Mpro protein. The MMGBSA scores of these compounds are highly promising, and they should be investigated to determine their potential as Mpro inhibitors, beneficial for COVID-19 treatment. In this study, we highlight the crucial role of in silico technologies in the search for novel therapeutic components. Computational biology, combined with structural biology, makes drug discovery studies more rigorous and reliable, and it creates a scenario where researchers can use existing drug components to discover new roles as modulators or inhibitors for various therapeutic targets. This study demonstrated that computational analyses can yield promising findings in the search for potential drug components. This work demonstrated the significance of increasing in silico and wetlab research to generate improved structure-based medicines.
Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies
Authors: Iorhemen Oliver Terna , Ukaigwe Sandra , Dang Hongyu , Liu Yang
Published: July 2022

Aerobic granular sludge (AGS) is known for high phosphorus removal from wastewaters, and phosphorus can be recovered from high phosphorus-containing waste sludge granules. This study aimed at determining the feeding strategy that provides the best performance in terms of the proliferation of polyphosphate-accumulating organisms (PAOs) and phosphorus removal. Using three AGS bioreactors, this study compared phosphorus removal and the proliferation dynamics of PAOs under three different feeding strategies: anaerobic slow feeding (R1), pulse feeding + anaerobic mixing (R2), and pulse feeding (R3). Results indicate that R1 and R2 achieved significantly higher phosphorus removal (97.6 ± 3% for R1 and 98.3 ± 1% for R2) than R3 (55 ± 11%). The anaerobic slow feeding procedure (R1) achieved the highest specific phosphorus release rate (SPRR) and specific phosphorus uptake rate (SPUR) as compared to the other two feeding conditions. 16S ribosomal ribonucleic acid (rRNA) gene sequencing assay of the microbial community for the three feeding strategies indicated that although the feeding strategy impacted reactor performance, it did not significantly alter the microbial community. The bacteria community composition maintained a similar degree of diversity. Proteobacteria, Bacteroidetes, and Verrucomicrobia were the dominant bacterial phyla in the system. Dominant PAOs were from the class Betaproteobacteria and the genera Paracoccus and Thauera. Glycogen-accumulating organisms were significantly inhibited while other less-known bacteria such as Wandonia and Hyphomonas were observed in all three reactors.
Multi-Objective Optimal Scheduling for Multi-Renewable Energy Power System Considering Flexibility Constraints
Authors: Yang Lei , Huang Wei , Guo Cheng , Zhang Dan , Xiang Chuan , Yang Longjie , Wang Qianggang
Published: July 2022

As renewable energy penetration increases, the lack of flexibility in a multi-renewable power system can seriously affect its own economics and reliability. To address this issue, three objectives are considered in this study: power fluctuations on tie-line, operating cost, and curtailment rate of renewable energy. Presented also is an optimal day-ahead scheduling model based on the MREPS for distributed generations with flexibility constraints. The multi-objective particle swarm optimization (MOPSO) algorithm can be applied to obtain a set of Pareto non-dominated solutions for the day-ahead scheduling strategy with the proposed model. By using fuzzy comprehensive evaluation, the optimal compromise solution is determined in the set. The presented method sacrifices a small amount of economy and power fluctuation, but it can reduce the deviation between forecast and realized power fluctuations on the tie-line, while improving the utilization of renewable energy.
Drying Characteristics of a Combined Drying System of Low-Pressure Superheated Steam and Heat Pump
Authors: Yao Yuan , Lu Zhenneng , Gong Yulie , Guo Song , Xiao Chupeng , Hu Wenbo
Published: July 2022

The present study aimed at investigating the use of a drying system combining the concept of low-pressure superheated steam drying and heat pump drying for fish. The effects of various drying medium pressures on the temperature field, airflow field, drying time, equipment performance as well as the power consumption of the drying process were investigated and discussed. Four comparative tests with different initial pressures were carried out according to a specified drying process by the combined drying system. The results showed that when the vacuum was high, the temperature field and airflow field in the drying chamber were more uniform. Due to the poor heat transfer performance of the drying medium at high vacuum, the drying time increased with a decrease in initial pressure. It was also found that with the decrease in drying medium pressure, the power consumption of the heat pump and the axial fans was reduced, while the power consumption of the electric heater went up. Overall, the total power consumption is directly proportional to the drying medium pressure.
Toward Buddhist Womanism: Tonglen Practice in The Color Purple
Authors: Huang Zhi
Published: July 2022

Tonglen is a Tibetan Buddhist practice that aims at developing the practitioner’s bodhicitta. In this article, I argue that it not only finds expression in Alice Walker’s The Color Purple through the protagonist Celie, but adds more complexity to the womanist philosophy for which Walker has been ensconced in positions of influence. More specifically, Celie follows an implied Buddhist practice of tonglen; in the process of “taking in and sending out”, her bodhicitta has been generated and cultivated. Underlying her tonglen practice is Buddhist womanism demonstrating how African American women can survive the social oppression and injustice by way of acknowledging their own terrible afflictions, empathizing with those enduring intense suffering, male and female, extending their loving kindness, comprehending the absence of intrinsic entity and the principle of dependent origination, etc. In addition, the article suggests that the fight for the survival of the oppressed is a type of Buddhist practice in Walker’s Buddhist womanism.
Satellite-Based Monitoring on Green-Up Date for Optimizing the Rest-Grazing Period in Xilin Gol Grassland
Authors: Wang Boyu , Yan Huimin , Wen Xin , Niu Zhongen
Published: July 2022

Ecological degradation has occurred in global grasslands and has impaired their ecosystem services severely, so ecological conservation of grasslands should be focused more on the effectiveness of management measures. The green-up process decides the year-round forage yield and ecological conditions of grasslands. Adopting rest-grazing during the green-up process can guarantee a successful green-up, thus realizing more economic benefits without grassland degradation. Therefore, studies should pay more attention to whether the green-up process is really covered by the rest-grazing period or not. We analyze the spatiotemporal variations and the stability of the annual green-up date in Xilin Gol Grassland from 2000 to 2018 based on MODIS time series images and compare the green-up date with the rest-grazing period to assess the effectiveness of the rest-grazing policy. The results show that the green-up date of Xilin Gol Grassland had advanced 15 days on average because of the increasing trend of both temperature and precipitation during 2000~2018. The green-up date is mostly 120~130 d in the east, about 10 days earlier than the west (130~140 d) and 20 days earlier than in the central areas (140~150 d), also because of the spatial variations of temperature and precipitation. The coefficient of variation (CV) of the green-up date showed a significant negative correlation with precipitation, so the green-up date is more unstable in the arid areas. The rest-grazing period started more than 45 days earlier than the green-up date and failed to cover it in several years, which occurred more frequently in southern counties. The average green-up date appeared after rest-grazing started in over 98% of areas, and the time gap is 15~45 days in 88% of areas, which not only could not avoid grassland degradation effectively but also increased herdsmen’s life burden. This study aims to accurately grasp the temporal and spatial variations of the green-up date in order to provide references for adjusting a more proper rest-grazing period, thus promoting ecological conservation and sustainable development of animal husbandry.
An Improved Method for the Evaluation and Local Multi-Scale Optimization of the Automatic Extraction of Slope Units in Complex Terrains
Authors: Yang Zhongkang , Wei Jinbing , Deng Jianhui , Zhao Siyuan
Published: July 2022

Slope units (SUs) are sub-watersheds bounded by ridge and valley lines. A slope unit reflects the physical relationship between landslides and geomorphological features and is especially useful for landslide sensitivity modeling. There have been significant algorithmic advances in the automatic delineation of SUs. But the intrinsic difficulties of determining input parameters and correcting for unreasonable SUs have hindered their wide application. An improved method of the evaluation and local multi-scale optimization for the automatic extraction of SUs is proposed. The Sus’ groups more consistent with the topographic features were achieved through a stepwise approach from a global optimum to a local refining. First, the preliminary subdivisions of multiple SUs were obtained based on the r.slopeunit software. The optimal subdivision scale was obtained by a collaborative evaluation approach capable of simultaneously measuring objective minimum discrepancies and seeking a global optimum. Second, under the selected optimal scale, unreasonable SUs such as over-subdivided slope units (OSSUs) and under-subdivided slope units (USSUs) were further distinguished. The local average similarity (LS) metric for each SU was designed based on calculating the SU’s area, common boundary and neighborhood variability. The inflection points of the cumulative frequency curve of LS were calculated as the distinguishing intervals for those unrealistic SUs by maximum interclass variance threshold. Third, a new effective optimization mechanism containing the re-subdivision of USSUs and merging of OSSUs was put into effect. We thus obtained SUs composed of terrain subdivisions with multiple scales, which is currently one of the few available methods for non-single scales. The statistical distributions of density, size and shapes demonstrate the excellent performance of the refined SUs in capturing the variability of complex terrains. Benefiting from the sufficient integrating approach of diverse features for each object, it is a significant advantage that the processing object can be transferred from general entirety to each precise individual.