bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of polysaccharide from Lonicera japonica Thunb leaves and its application in nano-emulsion
Authors: Li Yongchao , Liu Benguo , Yang Jing , Sun Junliang , Ran Junjian , Liang Xinhong , Li Yinglin
Published: August 2023

The polysaccharides in honeysuckle leaves (PHL) were separated and characterized for the first time. The nano-emulsion stabilized by PHL and whey protein isolate (WPI) were also fabricated based on the ultrasonic method. The results indicated that PHL was mainly composed of glucose (47.40 mol%), galactose (19.21 mol%) and arabinose (20.21 mol%) with the weight-average molecular weight of 137.97 ± 4.31 kDa. The emulsifier concentration, WPI-to-PHL ratio, ultrasound power and ultrasound time had significant influence on the droplet size of PHL-WPI nano-emulsion. The optimal preparation conditions were determined as following: emulsifier concentration, 1.7%; WPI/PHL ratio, 3:1; ultrasonic power, 700 W; ultrasonic time, 7 min. Under the above conditions, the median diameter of the obtained nano-emulsion was 317.70 ± 5.26 nm, close to the predicted value of 320.20 nm. The protective effect of PHL-WPI emulsion on β-carotene against UV irradiation was superior to that of WPI emulsion. Our results can provide reference for the development of honeysuckle leaves.
Redlining−associated methylation in breast tumors: the impact of contemporary structural racism on the tumor epigenome
Authors: Miller-Kleinhenz Jasmine M. , Moubadder Leah , Beyer Kirsten M. , Zhou Yuhong , Gaglioti Anne H. , Collin Lindsay J. , Gohar Jazib , Do Whitney , Conneely Karen , Krishnamurti Uma , Gogineni Keerthi , Gabram-Mendola Sheryl , D’Angelo Olivia , Henry Kashari , Torres Mylin , McCullough Lauren E.
Published: August 2023

Purpose

Place-based measures of structural racism have been associated with breast cancer mortality, which may be driven, in part, by epigenetic perturbations. We examined the association between contemporary redlining, a measure of structural racism at the neighborhood level, and DNA methylation in breast tumor tissue.

Methods

We identified 80 Black and White women diagnosed and treated for a first-primary breast cancer at Emory University Hospitals (2008–2017). Contemporary redlining was derived for census tracts using the Home Mortgage Disclosure Act database. Linear regression models were used to calculate the association between contemporary redlining and methylation in breast tumor tissue. We also examined epigenetic age acceleration for two different metrics, regressing β values for each cytosine-phosphate-guanine dinucleotide (CpG) site on redlining while adjusting for covariates. We employed multivariable Cox-proportional hazards models and 95% confidence intervals (CI) to estimate the association between aberrant methylation and mortality.

Results

Contemporary redlining was associated with 5 CpG sites after adjustment for multiple comparisons (FDR<0.10). All genes were implicated in breast carcinogenesis, including genes related to inflammation, immune function and stress response (ANGPT1, PRG4 and PRG4). Further exploration of the top 25 CpG sites, identified interaction of 2 sites (MRPS28 and cg11092048) by ER status and 1 site (GDP1) was associated with all-cause mortality. Contemporary redlining was associated with epigenetic age acceleration by the Hannum metric (β=5.35; CI 95%=0.30,10.4) and showed positive but non-significant correlation with the other clock.

Conclusion

We identified novel associations between neighborhood contemporary redlining and the breast tumor DNA methylome, suggesting that racist policies leading to inequitable social and environmental exposures, may impact the breast tumor epigenome. Additional research on the potential implications for prognosis is needed.
Risk factors for extraurothelial recurrence in upper tract urothelial carcinoma after radical nephroureterectomy: a retrospective study based on a Chinese population
Authors: Luo Zhenkai , Jiao Binbin , Yan Yangxuanyu , Su Caixia , Pan Yijin , Zhao Hang , Bo Yuxuan , Zhang Guan , Ding Zhenshan
Published: August 2023

Objectives

The risk factors for extraurothelial recurrence (EUR) after radical nephroureterectomy (RNU) in patients with upper urinary tract urothelial carcinoma (UTUC) are currently inconsistent and unclear. In this study, we aimed to identify these risk factors and develop a grading system for EUR.

Methods

We retrospectively analyzed 220 patients who underwent RNU for UTUC in our center from January 2009 to December 2020. Overall survival (OS) and extraurothelial recurrence-free survival (EURFS) were compared using the Kaplan–Meier curve with a log-rank test. Univariate and multivariate Cox regression models were applied to identify the independent risk factors related to EUR.

Results

The median follow-up period was 42 (range: 2–143) months. Of the 220 patients, 61 patients developed EUR in our cohort, which had worse survival outcome. Multivariate Cox regression analysis showed pathologic stage, lymph node (LN) status, lymphovascular invasion (LVI), Ki-67, neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) were independent risk factors for EUR. The Kaplan–Meier curves revealed a significant difference in EUR among the three risk groups.

Conclusion

Our study suggests that pathologic stage, LN status, LVI, Ki-67, NLR, and PLR are independent risk factors for EUR in UTUC patients after RNU. The development of a grading system for EUR risk stratification may assist urologists in making clinical decisions regarding the management of UTUC.
Molecular mechanism of palmitic acid and its derivatives in tumor progression
Authors: Wang Xitan , Zhang Chaonan , Bao Na
Published: August 2023

Palmitic acid (PA) is a saturated fatty acid commonly found in coconut oil and palm oil. It serves as an energy source for the body and plays a role in the structure and function of cell membranes. Beyond its industrial applications, PA has gained attention for its potential therapeutic properties. Modern pharmacological studies have demonstrated that PA exhibits anti-inflammatory, antioxidant, and immune-enhancing effects. In recent years, PA has emerged as a promising anti-tumor agent with demonstrated efficacy against various malignancies including gastric cancer, liver cancer, cervical cancer, breast cancer, and colorectal cancer. Its anti-tumor effects encompass inducing apoptosis in tumor cells, inhibiting tumor cell proliferation, suppressing metastasis and invasion, enhancing sensitivity to chemotherapy, and improving immune function. The main anticancer mechanism of palmitic acid (PA) involves the induction of cell apoptosis through the mitochondrial pathway, facilitated by the promotion of intracellular reactive oxygen species (ROS) generation. PA also exhibits interference with the cancer cell cycle, leading to cell cycle arrest predominantly in the G1 phase. Moreover, PA induces programmed cell autophagy death, inhibits cell migration, invasion, and angiogenesis, and synergistically enhances the efficacy of chemotherapy drugs while reducing adverse reactions. PA acts on various intracellular and extracellular targets, modulating tumor cell signaling pathways, including the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), endoplasmic reticulum (ER), B Cell Lymphoma-2 (Bcl-2), P53, and other signaling pathways. Furthermore, derivatives of PA play a significant regulatory role in tumor resistance processes. This paper provides a comprehensive review of recent studies investigating the anti-tumor effects of PA. It summarizes the underlying mechanisms through which PA exerts its anti-tumor effects, aiming to inspire new perspectives for the treatment of malignant tumors in clinical settings and the development of novel anti-cancer drugs.
Interferonopathies masquerading as non-Mendelian autoimmune diseases: pattern recognition for early diagnosis
Authors: Gagne Samuel , Sivaraman Vidya , Akoghlanian Shoghik
Published: August 2023

Type I interferonopathies are a broad category of conditions associated with increased type I interferon gene expression and include monogenic autoinflammatory diseases and non-Mendelian autoimmune diseases such as dermatomyositis and systemic lupus erythematosus. While a wide range of clinical presentations among type I interferonopathies exists, these conditions often share several clinical manifestations and implications for treatment. Presenting symptoms may mimic non-Mendelian autoimmune diseases, including vasculitis and systemic lupus erythematosus, leading to delayed or missed diagnosis. This review aims to raise awareness about the varied presentations of monogenic interferonopathies to provide early recognition and appropriate treatment to prevent irreversible damage and improve quality of life and outcomes in this unique patient population.
Effectiveness and safety of Lacosamide therapy for children with focal epilepsy: a real world study
Authors: Yang Chunsong , Liu Zheng , Peng Yuxuan , Zhang Lingli , Yu Dan
Published: August 2023

Objectives: To compare the effectiveness and safety of the new antiepileptic drug, lacosamide (LCM) with Levetiracetam, for the treatment of focal epilepsy in children.

Methods: This study was a cohort study. Children with focal epilepsy who received LCM or Levetiracetam treatment in West China Second Hospital of Sichuan University were recruited and followed up for 12 months. Changes in the frequency of epilepsy, 50% and 75% responder rates, and seizure freedom rates from baseline to the maintenance period and adherence score were assessed. In addition, adverse events (AEs) were recorded.

Results: 92 patients completed the study, and were divided into two groups: LCM (n = 46) and Levetiracetam (n = 46). Participants were aged from 2 to 16.3 years, with a mean epilepsy duration of 2.57 years. The average maintenance dose of LCM was 5.03 ± 1.91 mg/kg/d after the titration period. There was no significant difference between the two groups in terms of the mean seizure frequency during subsequent visits at 1, 3,6, 9, 12 months. There was significant difference between the two groups in terms of the 50% responder rate at 6 months. No serious AEs were reported in both groups. The vast majority of patients had good adherence (adherence score = 4) in the LCM group.

Conclusion: LCM is effective as adjunctive therapy in children with epilepsy and has good safety, tolerability and adherence. Large sample size studies with long-term follow-up are needed in the future to comprehensively evaluate the use of LCM in children.

Clinical Trial Registration: [https://www.chictr.org.cn/showproj.html?proj=41041], identifier [ChiCTR1900024507].
Protective effects and possible mechanisms of catalpol against diabetic nephropathy in animal models: a systematic review and meta-analysis
Authors: Fu Zhongmei , Su Xiaojuan , Zhou Qi , Feng Haoyue , Ding Rui , Ye Hejiang
Published: August 2023

Aim of the Study:Rehmannia glutinosa is a core Chinese herbal medicine for the treatment of diabetes and diabetic nephropathy (DN). It has been used for the treatment of diabetes for over 1,000 years. Catalpol is the main active compound in Rehmannia roots. Current evidence suggests that catalpol exhibits significant anti-diabetic bioactivity, and thus it has attracted increasing research attention for its potential use in treating DN. However, no studies have systematically evaluated these effects, and its mechanism of action remains unclear. This study aimed to evaluate the effects of catalpol on DN, as well as to summarize its possible mechanisms of action, in DN animal models.

Materials and Methods: We included all DN-related animal studies with catalpol intervention. These studies were retrieved by searching eight databases from their dates of inception to July 2022. In addition, we evaluated the methodological quality of the included studies using the Systematic Review Center for Laboratory animal Experimentation (SYRCLE) risk-of-bias tool. Furthermore, we calculated the weighted standard mean difference (SMD) with 95% confidence interval (CI) using the Review Manager 5.3 software and evaluated publication bias using the Stata (12.0) software. A total of 100 studies were retrieved, of which 12 that included 231 animals were finally included in this review.

Results: As compared to the control treatment, treatment with catalpol significantly improved renal function in DN animal models by restoring serum creatinine (Scr) (p = 0.0009) and blood urea nitrogen (BUN) (p < 0.00001) levels, reducing proteinuria (p < 0.00001) and fasting blood glucose (FBG) (p < 0.0001), improving kidney indices (p < 0.0001), and alleviating renal pathological changes in the animal models. In addition, it may elicit its effects by reducing inflammation and oxidative stress, improving podocyte apoptosis, regulating lipid metabolism, delaying renal fibrosis, and enhancing autophagy.

Conclusion: The preliminary findings of this preclinical systematic review suggest that catalpol elicits significant protective effects against hyperglycemia-induced kidney injury. However, more high-quality studies need to be carried out in the future to overcome the methodological shortcomings identified in this review.
Derivatives of 9-phosphorylated acridine as butyrylcholinesterase inhibitors with antioxidant activity and the ability to inhibit β-amyloid self-aggregation: potential therapeutic agents for Alzheimer’s disease
Authors: Makhaeva Galina F. , Kovaleva Nadezhda V. , Rudakova Elena V. , Boltneva Natalia P. , Lushchekina Sofya V. , Astakhova Tatiana Yu , Timokhina Elena N. , Serebryakova Olga G. , Shchepochkin Alexander V. , Averkov Maxim A. , Utepova Irina A. , Demina Nadezhda S. , Radchenko Eugene V. , Palyulin Vladimir A. , Fisenko Vladimir P. , Bachurin Sergey O. , Chupakhin Oleg N. , Charushin Valery N. , Richardson Rudy J.
Published: August 2023

We investigated the inhibitory activities of novel 9-phosphoryl-9,10-dihydroacridines and 9-phosphorylacridines against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and carboxylesterase (CES). We also studied the abilities of the new compounds to interfere with the self-aggregation of β-amyloid (Aβ42) in the thioflavin test as well as their antioxidant activities in the ABTS and FRAP assays. We used molecular docking, molecular dynamics simulations, and quantum-chemical calculations to explain experimental results. All new compounds weakly inhibited AChE and off-target CES. Dihydroacridines with aryl substituents in the phosphoryl moiety inhibited BChE; the most active were the dibenzyloxy derivative 1d and its diphenethyl bioisostere 1e (IC50 = 2.90 ± 0.23 µM and 3.22 ± 0.25 µM, respectively). Only one acridine, 2d, an analog of dihydroacridine, 1d, was an effective BChE inhibitor (IC50 = 6.90 ± 0.55 μM), consistent with docking results. Dihydroacridines inhibited Aβ42 self-aggregation; 1d and 1e were the most active (58.9% ± 4.7% and 46.9% ± 4.2%, respectively). All dihydroacridines 1 demonstrated high ABTS•+-scavenging and iron-reducing activities comparable to Trolox, but acridines 2 were almost inactive. Observed features were well explained by quantum-chemical calculations. ADMET parameters calculated for all compounds predicted favorable intestinal absorption, good blood–brain barrier permeability, and low cardiac toxicity. Overall, the best results were obtained for two dihydroacridine derivatives 1d and 1e with dibenzyloxy and diphenethyl substituents in the phosphoryl moiety. These compounds displayed high inhibition of BChE activity and Aβ42 self-aggregation, high antioxidant activity, and favorable predicted ADMET profiles. Therefore, we consider 1d and 1e as lead compounds for further in-depth studies as potential anti-AD preparations.
Tracking performance and its underlying characteristics in talented swimmers: a longitudinal study during the junior-to-senior transition
Authors: Post Aylin K. , Koning Ruud H. , Visscher Chris , Elferink-Gemser Marije T.
Published: August 2023

The present study strived to gain a more profound understanding of the distinctions in development between swimmers who are considered to be on track to the senior elite level compared to those who are not. Longitudinal data of 29 talented sprint and middle-distance swimmers (12 males; 17 females) on season best performances (season best times) and underlying performance characteristics (anthropometrics, starts, turns, maximal swimming velocity, stroke index [SI, an indirect measure of swimming efficiency] and lower body power) were collected over four swimming seasons (median of n = 3 seasons per swimmer). Based on their season best performance at early senior age (males aged 18–19; females aged 17–18), some swimmers were considered to be on track to reach the elite level (referred to as high-performing seniors; 6 males and 10 females), whereas others were not (referred to as lower-performing seniors; 6 males and 7 females). Retrospectively studying these swimmers (males and females separately), we found that all high-performing seniors were already on track to the elite level at late junior age (males aged 17; females aged 16), evidenced with faster season best performances throughout their transition compared to their lower-performing peers (p < 0.05). Independent sample t-tests revealed that high-performing seniors significantly outscored their lower-performing peers on maximal swimming velocity (males and females), starts and turns (males), SI (females) and lower body power (females) at late junior age (p < 0.05). Additionally, multilevel models showed faster rates of development for high-performing seniors on turns and maximal swimming velocity (males), and SI (females) compared to lower-performing peers during the junior-to-senior transition (p < 0.05). Particularly, the higher initial levels of swim performance and underlying characteristics at late junior age as well as the ability to keep progressing on season best performances (males and females), turns and maximal swimming velocity (males), and SI (females) during the junior-to-senior transition, may be crucial factors in the attainment of swimming expertise.
Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus
Authors: Istiandari Pramesti , Yasumoto Shuhei , Seki Hikaru , Fukushima Ery Odette , Muranaka Toshiya
Published: August 2023

Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in the structural diversification of triterpenoids. To perform site-specific oxidations of the triterpene scaffold, CYPs require electrons transferred by NADPH-cytochrome P450 reductase (CPR), which is classified into two main classes, class I and class II, based on their structural difference. Lotus japonicus is a triterpenoids-producing model legume with one CPR class I gene (LjCPR1) and a minimum of two CPR class II genes (LjCPR2-1 and LjCPR2-2). CPR classes I and II from different plants have been reported to be involved in different metabolic pathways. By performing gene expression analyses of L. japonicus hairy root culture treated with methyl jasmonate (MeJA), this study revealed that LjCPR1, CYP716A51, and LUS were down-regulated which resulted in no change in betulinic acid and lupeol content. In contrast, LjCPR2s, bAS, CYP93E1, and CYP72A61 were significantly upregulated by MeJA treatment, followed by a significant increase of the precursors for soyasaponins, i.e. β-amyrin, 24-OH β-amyrin, and sophoradiol content. Triterpenoids profile analysis of LORE1 insertion and hairy root mutants showed that the loss of the Ljcpr2-1 gene significantly reduced soyasaponins precursors but not in Ljcpr1 mutants. However, Ljcpr1 and Ljcpr2-1 mutants showed a significant reduction in lupeol and oleanolic, ursolic, and betulinic acid contents. Furthermore, LjCPR1, but not LjCPR2, was crucial for seed development, supporting the previous notion that CPR class I might support plant basal metabolism. This study suggests that CPR classes I and II play different roles in L. japonicus triterpenoid biosynthesis.
The relationship between non-suicidal self-injury and childhood abuse in transgender people: a cross-sectional cohort study
Authors: Cao Qiang , Zhang Qi , Chen Yuquan , He Zixu , Xiang Zhibiao , Guan Haoran , Yan Na , Qiang Yi , Li Mantao
Published: August 2023

Objective

To explore the relationship between non-suicidal self-injury (NSSI) and childhood abuse in transgender people and the mediating effect of emotional dysregulation traits in the association between childhood abuse and non-suicidal self-injury.

Patients and methods

From May to October 2021, 296 female-to-male (FTM) and 675 male-to-females (MTF), with age of 24.5 ± 6.4 years, were recruited using peer-driven sampling and anonymous questionnaires in Guangdong Province. The Childhood Abuse Questionnaire (CTQ-SF), the Personality Diagnostic Questionnaire (PDQ-4+) emotion regulation ability scale and the DSM-5 Clinical Examination of Stereotypic Disorders were used to measure childhood abuse experiences, emotional dysregulation traits and self-injurious behaviour, respectively.

Results

Childhood abuse scores were positively correlated with both emotional dysregulation traits scores and non-suicidal self-injury (NSSI) behaviours (p < 0.01), and emotional dysregulation traits scores were positively correlated with NSSI behaviours (p < 0.01); emotional dysregulation traits partially mediated the association between childhood abuse and NSSI behaviours, with the mediating effect accounting for 23.23% of the total effect. In addition, among the factors of childhood abuse, emotional dysregulation traits mediated the association between emotional abuse, emotional neglect, sexual abuse, physical abuse, physical neglect and NSSI behaviour significantly, with the mediating effect accounting for 22.48%–32.58% of the total effect.

Conclusion

Transgender NSSI behaviours are associated with childhood abuse and emotional dysregulation traits, and emotional dysregulation traits partially mediates the association between childhood abuse and NSSI behaviours, and screening for emotional dysregulation traits in transgender people and timely interventions are needed to improve the current situation of discrimination against transgender people.
Responses in cognitive hierarchy games are correlated with academic performance and the cognitive reflection test
Authors: Mantilla César , Ortiz-Merchán Silvia
Published: August 2023

Economics and Finance undergraduate students from four cohorts played LUPI, a game rewarding the person submitting the lowest unique positive integer, for a small bonus in an exam. Some months later, they played this game again with financial incentives and took a cognitive reflection test (CRT). We find that submitted responses to different configurations of LUPI are correlated with short-term (i.e., exam grade) and medium-term (i.e., final grade and GPA) academic performance, as well as the score in the CRT.
The Italian language postpartum specific anxiety scale [PSAS-IT]: translation, psychometric evaluation, and validation
Authors: Ionio Chiara , Ciuffo Giulia , Landoni Marta , Smorti Martina , Della Vedova Anna Maria , Christiansen Paul , Fallon Victoria , Silverio Sergio A. , Bramante Alessandra
Published: August 2023

Introduction

While often positive, the lifecourse transition to motherhood is susceptible to the risk for developing mood disorders. Postpartum anxiety has often been overshadowed by other perinatal-specific mental health disorders, such as postpartum depression, and therefore has not been at the forefront or center of as much empirical study. This has meant there is a lack of effective and reliable tools with which to measure it, despite growing evidence suggesting its detrimental impact on mothers, their babies, wider family and social contacts, and on healthcare systems. This current study aimed to translate and validate the Postpartum Specific Anxiety Scale [PSAS] into the Italian language, and to validate the tool for its use in detecting anxiety specific to motherhood.

Methods

The study (N = 457) comprised 4 stages: English-Italian translation and back-translation to obtain the Italian version [PSAS-IT]; a preliminary pilot study to adapt the PSAS to the characteristics of the Italian population; measurement invariance; and internal reliability of subscales.

Results

The PSAS-IT demonstrates similar psychometric properties as the original English-language PSAS, with acceptable acceptability, construct and convergent validity, and internal consistency. Confirmatory factor analysis for multiple groups (Italy and United Kingdom) showed that the factor structure of the PSAS was valid for both groups [χ2 (2436) = 4679.481, p < 0.001, TLI = 0.969, CFI =0.972, RMSEA = 0.045, SRMR =0.064].

Discussion

The resulting findings offer a reliable measure of postpartum anxiety in Italian language up to six months after birth.
Physical and sexual victimization of persons with severe mental illness seeking care in central and southwestern Uganda
Authors: Mpango Richard Stephen , Ssembajjwe Wilber , Rukundo Godfrey Zari , Amanyire Philip , Birungi Carol , Kalungi Allan , Rutakumwa Rwamahe , Tusiime Christine , Gadow Kenneth D. , Patel Vikram , Nyirenda Moffat , Kinyanda Eugene
Published: August 2023

Purpose

This study established the prevalence of physical and sexual victimization, associated factors and psychosocial consequences of victimization among 1,201 out-patients with severe mental illness at Butabika and Masaka hospitals in Uganda.

Methods

Participants completed structured, standardized and locally translated instruments. Physical and sexual victimization was assessed using the modified adverse life events module of the European Para-suicide Interview Schedule. We used logistic regression to determine the association between victimization, the associated factors and psychosocial consequences.

Results

The prevalence of physical abuse was 34.1% and that of sexual victimization was 21.9%. The age group of > = 50 years (aOR 1.02;95% CI 0.62–1.66; p = 0.048) was more likely to have suffered physical victimization, while living in a rural area was protective against physical (aOR 0.59; 95% CI 0.46–0.76; p = <0.001) and sexual (aOR 0.48, 95% CI 0.35–0.65; p < 0.001) victimization. High socioeconomic status (SES) (aOR 0.56; 95% CI 0.34–0.92; p = <0.001) was protective against physical victimization. Females were more likely to have been sexually victimized (aOR 3.38; 95% CI 2.47–4.64; p = <0.001), while being a Muslim (aOR 0.60; 95% CI 0.39–0.90; p = 0.045) was protective against sexual victimization. Risky sexual behavior was a negative outcome associated with physical (aOR 2.19; 95% CI 1.66–2.90; p = <0.001) and sexual (aOR 3.09; 95% CI 2.25–4.23; p < 0.001) victimization. Mental health stigma was a negative outcome associated with physical (aOR 1.03; 95% CI 1.01–1.05; p < 0.001) and sexual (aOR 1.03; 95% CI 1.01–1.05; p = 0.002) victimization. Poor adherence to oral anti-psychotic medications was a negative outcome associated with physical (aOR 1.51; 95% CI 1.13–2.00; p = 0.006) and sexual (aOR 1.39; 95% CI 0.99–1.94; p = 0.044) victimization.

Conclusion

There is a high burden of physical and sexual victimization among people with SMI in central Uganda. There is need to put in place and evaluate complex interventions for improving detection and response to abusive experiences within mental health services. Public health practitioners, policymakers, and legislators should act to protect the health and rights of people with SMI in resource poor settings.
Environmental factors differentially affect epistaxis among preschool and school-aged children
Authors: Ahn Eun-Jin , Min Hyun Jin
Published: August 2023

Introduction

Environmental factors are closely associated with pediatric epistaxis. Whether this association differs according to age has not been previously reported. Therefore, we tried to evaluate the differences in associations between environmental factors and epistaxis in children of different ages.

Methods

A total of 20,234 patients with epistaxis who visited the hospital between January 1, 2002, and December 31, 2015, were enrolled in this study. The patients were divided into two groups according to their ages: preschool-aged (<6 years) and school-aged children (6–18 years). Daily, monthly, and yearly data on environmental factors were collected. We performed a stepwise logistic regression to identify the potential environmental risk factors for epistaxis in each age group.

Results

The mean number of epistaxis cases per month in both groups was highest in September. The cases were lowest in February in preschool-aged children and in November in school-aged children. Temperature, humidity, maximum wind speed, and sunshine duration were associated with epistaxis in preschool-aged children. Average wind speed, particulate matter (>10 μm diameter), temperature, humidity, sunshine duration, and sulfur dioxide concentration were associated with epistaxis in school-aged children.

Conclusion

This study indicates that the differences in environmental risk factors for epistaxis are associated with the patient’s age.
Optimal predictive neuro-navigator design for mobile robot navigation with moving obstacles
Authors: Mohaghegh Mahsa , Saeedinia Samaneh-Alsadat , Roozbehi Zahra
Published: August 2023

Introduction: The challenge of navigating a Mobile robot in dynamic environments has grasped significant attention in recent years. Despite the available techniques, there is still a need for efficient and reliable approaches that can address the challenges of real-time near optimal navigation and collision avoidance.

Methods: This paper proposes a novel Log-concave Model Predictive Controller (MPC) algorithm that addresses these challenges by utilizing a unique formulation of cost functions and dynamic constraints, as well as a convergence criterion based on Lyapunov stability theory. The proposed approach is mapped onto a novel recurrent neural network (RNN) structure and compared with the CVXOPT optimization tool. The key contribution of this study is the combination of neural networks with model predictive controller to solve optimal control problems locally near the robot, which offers several advantages, including computational efficiency and the ability to handle nonlinear and complex systems.

Results: The major findings of this study include the successful implementation and evaluation of the proposed algorithm, which outperforms other methods such as RRT, A-Star, and LQ-MPC in terms of reliability and speed. This approach has the potential to facilitate real-time navigation of mobile robots in dynamic environments and ensure a feasible solution for the proposed constrained-optimization problem.
Bovine sperm HSP-70 molecules: a potential cryo-tolerance marker associated with semen quality and fertility rate
Authors: Pardede Berlin Pandapotan , Kusumawati Asmarani , Pangestu Mulyoto , Purwantara Bambang
Published: August 2023

Introduction

Freezability is the ability of sperm to maintain its vitality and quality from various stress during the cryopreservation process, which is very important for the success of fertilization in AI programs. Heat shock proteins (HSPs) are unique proteins induced in response to various stress, including excess reactive oxygen species (ROS) and oxidative damage to intracellular enzymes that can harm cells. This study aimed to analyze the potential of HSP-70 molecules in bovine sperm as a marker of freezability or cryo-tolerance, as well as its association with semen quality and fertility rate.

Methods

The classification of bulls is based on freezability (good freezability/GF and poor freezability/PF), which is obtained from the value of post-thaw viability using the SYBR-14/PI-flow cytometry. Semen quality assessed included sperm motility and kinetics (computer-assisted sperm analyses), plasma membrane integrity (HOS test), acrosome integrity (FITC-PNA), mitochondrial membrane (JC-1), and DNA damage (Halomax kit). The bull fertility rate assessment was analyzed based on the first service conception rate of each bull derived from data on the success of artificial insemination contained in the Indonesian-integrated National Animal Health Information System (iSIKHNAS). Gene expression levels of HSP-70 bovine sperm were performed using the RT-qPCR method. The protein abundance of HSP-70 bovine sperm was determined using the enzyme immunoassay (EIA) method.

Results

Bovine sperm HSP-70 molecules, at the gene and protein level, showed a higher abundance in GF (p < 0.05) than in PF bulls. The percentage of each parameter of frozen–thawed sperm quality was significantly higher in GF (p < 0.05) than in PF bulls. The HSP-70 molecules at the gene and protein levels were significantly positively correlated (p < 0.01) with the fertility rate. Furthermore, HSP-70 molecules were negatively associated (p < 0.01) with low mitochondrial membrane potential and sperm DNA damage and positively correlated (p < 0.01) with other frozen–thawed sperm quality parameters. The overall quality of frozen–thawed sperm was closely related (p < 0.01) to the fertility rate.

Conclusion

We may conclude that HSP-70 molecules in bovine sperm at the gene and protein level have the potential to be developed as a marker for cryo-tolerance or freezability, which may be utilized as a predictor of fertility and frozen–thawed sperm quality in bulls.
Modulation of digestibility of canine food using enzyme supplement: an in vitro simulated semi-dynamic digestion study
Authors: Jadhav Swati , Gaonkar Tejal , Joshi Mithila , Rathi Abhijit
Published: August 2023

Digestibility and nutrient availability are important parameters when estimating the nutritional quality of pet food. We have developed a simulated semi-dynamic in vitro canine digestion model to evaluate the digestibility of dry extruded canine food. Canine food was assessed for digestible energy, dry matter digestibility, protein digestibility, non-fibrous carbohydrate (NFC) digestibility, and total antioxidant capacity (TAC) in the absence and presence of an enzyme blend (DigeSEB Super Pet). Enzyme blend supplementation in canine food was found to increase the dry matter digestibility (18.7%, p < 0.05), digestible energy (18.1%, p < 0.05), and protein digestibility (11%, p < 0.1) and reducing sugar release (106.3%, p < 0.005). The release of low molecular weight peptides (48.7%) and essential amino acids (15.6%) increased within 0.5 h of gastrointestinal digestion due to enzyme blend supplementation. Furthermore, the TAC of the digesta was also increased (8.1%, p < 0.005) in the canine food supplemented with enzyme blend. Overall, supplementation of enzyme blend in canine food is an effective strategy to enhance the food digestibility and nutrient availability for absorption.
Recent Developments in the Aerodynamic Heat Transfer and Cooling Technology of Gas Turbines Endwalls
Authors: Zhang Rongdi , Liu Pengchao , Zhang Xirui , Xi Wenxiong , Liu Jian
Published: August 2023

With an increased inlet gas temperature and the homogenization of the combustion chamber outlet temperature, the endwalls of gas turbines are exposed to extremely high heat loads. The complex flow structure of turbine endwalls makes it difficult to cool some regions of the endwalls, which can easily cause endwall ablation, reducing turbine aerodynamic performance and threatening the turbine’s safe operation. In order to improve the cooling and aerodynamic performance of gas turbines, the flow structure, heat transfer and film cooling characteristics of endwalls are analyzed in depth in this paper. This paper summarizes and analyzes the development of the aerodynamic heat transfer and film cooling of gas turbine endwalls in terms of geometric structures and flow boundary conditions and also presents new research directions. Based on the literature, the development and challenge of turbine endwall film cooling are also discussed.
CLT-YOLOX: Improved YOLOX Based on Cross-Layer Transformer for Object Detection Method Regarding Insect Pest
Authors: Zhang Lijuan , Cui Haibin , Sun Jiadong , Li Zhiyi , Wang Hao , Li Dongming
Published: August 2023

This paper presents an enhanced YOLOX-based algorithm for pest detection, adopting a nature-inspired approach for refining its methodology. To tackle the limited availability of image data pertaining to pests and diseases, the paper incorporates Mosaic and Mixup technologies for effective image preprocessing. Furthermore, a novel training strategy is proposed to enhance the overall quality of the results. The existing architecture is enriched by integrating shallow information, while the CLT module is devised to facilitate cross-layer fusion and extract essential feature information. This advancement enables improved object detection across various scales. Additionally, the paper optimizes the original PFPN structure by eliminating the convolutional layer preceding upsampling, enhancing the C3 module, and integrating the convolutional attention model (CBAM) to identify salient regions within complex scenes. The performance of the proposed CLT-YOLOX model is extensively evaluated using the IP102 dataset, demonstrating its effectiveness. Notably, the model exhibits significant improvements compared to the original AP evaluation index, with an increase of 2.2% in average precision (mAP) and 1.8% in AP75. Furthermore, favorable results are achieved in the COCOmAP index, particularly in the APsmall category where there is a 2.2% improvement in performance.
It Is Not Only Data—Freshwater Invertebrates Misused in Biological Monitoring
Authors: Koperski Paweł
Published: August 2023

The article presents and discusses the issues of the use of free-living invertebrates to assess the ecological status of freshwater environments with different methods of biological monitoring. Invertebrates are excluded from ethical consideration in the procedures of environmental protection, which results in the killing of many more individuals during sampling than necessary. Biomonitoring is used as a routine method for environmental protection that results in the cruel death of even millions of aquatic animals annually. In many cases, the mortality of animals used in such types of activities has been shown as excessive, e.g., because the vast majority die due to unnecessary subsampling procedures. Improperly planned and conducted procedures which result in excessive mortality have or may have a negative impact on the environment and biodiversity. Their existence as sensitive beings is reduced to an information function; they become only data useful for biomonitoring purposes. The main problem when trying to determine the mortality of invertebrates due to biomonitoring activities and its impact on natural populations seems to be the lack of access to raw data presenting how many animals were killed during sampling.
A Pilot Study on the Feasibility of an Extended Suckling System for Pasture-Based Dairies
Authors: Ospina Rios Sandra Liliana , Lee Caroline , Andrewartha Sarah J. , Verdon Megan
Published: August 2023

This study investigated cow-calf productivity in a 10-week, pasture-based, extended suckling system featuring part-time cow-calf contact and once-a-day milking. A total of 30 dairy cows and their calves were assigned to two treatments: (1) cow and calf managed in an extended suckling system; or (2) cow and calf separated at birth and managed as usual. Cow-calf pairs grazed together during the day and spent the night separated by fence-line contact. The dams were reunited with the calves after once-a-day milking every morning. The commercial treatment pairs were separated after birth, and cows were milked twice a day and managed within the farm herd. Commercial calves were reared and managed as per commercial Australian practices. Cow-calf dams yielded 9 L/cow/day less saleable milk (p < 0.001), and their milk had lower fat (p = 0.04) but a higher protein percentage (p < 0.001) than commercial cows during pre-weaning. However, milk yield and composition were comparable post-weaning. Dam-suckled calves gained weight faster and were therefore weaned 2 weeks earlier than commercial calves, which were offered 8 L/day milk. This study has demonstrated a novel system of extended cow-calf suckling that could be practical to implement in pasture-based dairies. The long-term effects and scalability of the extended suckling system described here require further validation.
Using Reinforcement Learning to Solve a Dynamic Orienteering Problem with Random Rewards Affected by the Battery Status
Authors: Juan Angel A. , Marugan Carolina A. , Ahsini Yusef , Fornes Rafael , Panadero Javier , Martin Xabier A.
Published: August 2023

This paper discusses an orienteering optimization problem where a vehicle using electric batteries must travel from an origin depot to a destination depot while maximizing the total reward collected along its route. The vehicle must cross several consecutive regions, with each region containing different types of charging nodes. A charging node has to be selected in each region, and the reward for visiting each node—in terms of a ‘satisfactory’ charging process—is a binary random variable that depends upon dynamic factors such as the type of charging node, weather conditions, congestion, battery status, etc. To learn how to efficiently operate in this dynamic environment, a hybrid methodology combining simulation with reinforcement learning is proposed. The reinforcement learning component is able to make informed decisions at each stage, while the simulation component is employed to validate the learning process. The computational experiments show how the proposed methodology is capable of design routing plans that are significantly better than non-informed decisions, thus allowing for an efficient management of the vehicle’s battery under such dynamic conditions.
Application of an Enhanced Whale Optimization Algorithm on Coverage Optimization of Sensor
Authors: Xu Yong , Zhang Baicheng , Zhang Yi
Published: August 2023

The wireless sensor network (WSN) is an essential technology of the Internet of Things (IoT) but has the problem of low coverage due to the uneven distribution of sensor nodes. This paper proposes a novel enhanced whale optimization algorithm (WOA), incorporating Lévy flight and a genetic algorithm optimization mechanism (WOA-LFGA). The Lévy flight technique bolsters the global search ability and convergence speed of the WOA, while the genetic optimization mechanism enhances its local search and random search capabilities. WOA-LFGA is tested with 29 mathematical optimization problems and a WSN coverage optimization model. Simulation results demonstrate that the improved algorithm is highly competitive compared with mainstream algorithms. Moreover, the practicality and the effectiveness of the improved algorithm in optimizing wireless sensor network coverage are confirmed.
Molybdenum Disulfide-Integrated Iron Organic Framework Hybrid Nanozyme-Based Aptasensor for Colorimetric Detection of Exosomes
Authors: Li Chao , Guo Zichao , Pu Sisi , Zhou Chaohui , Cheng Xi , Zhao Ren , Jia Nengqin
Published: August 2023

Tumor-derived exosomes are considered as a potential marker in liquid biopsy for malignant tumor screening. The development of a sensitive, specific, rapid, and cost-effective detection strategy for tumor-derived exosomes is still a challenge. Herein, a visualized and easy detection method for exosomes was established based on a molybdenum disulfide nanoflower decorated iron organic framework (MoS2-MIL-101(Fe)) hybrid nanozyme-based CD63 aptamer sensor. The CD63 aptamer, which can specifically recognize and capture tumor-derived exosomes, enhanced the peroxidase activity of the hybrid nanozyme and helped to catalyze the 3,3′,5,5′-tetramethylbenzidine (TMB)-H2O2 system to generate a stronger colorimetric signal, with its surface modification on the hybrid nanozyme. With the existence of exosomes, CD63 aptamer recognized and adsorbed them on the surface of the nanozyme, which rescued the enhanced peroxidase activity of the aptamer-modified nanozyme, resulting in a deep-to-moderate color change in the TMB-H2O2 system where the change is visible and can be monitored with ultraviolet-visible spectroscopy. In the context of optimal circumstances, the linear range of this exosome detection method is measured to be 1.6 × 104 to 1.6 × 106 particles/μL with a limit of detection as 3.37 × 103 particles/μL. Generally, a simple and accessible approach to exosome detection is constructed, and a nanozyme-based colorimetric aptamer sensor is proposed, which sheds light on novel oncological biomarker measurements in the field of biosensors.
The Effect of Fiber End on the Bonding Mechanical Properties between SMA Fibers and ECC Matrix
Authors: Yang Zhao , Deng Tingyu , Fu Qingshi
Published: August 2023

In order to investigate the effect of fiber end on the bonding mechanical properties between shape memory alloy (SMA) fibers and Engineered Cementitious Composites (ECC), this study designed and fabricated five groups of specimens with variations in SMA fiber end shape, diameter and depth-to-diameter ratio. Direct tensile tests were conducted on these specimens under displacement control. The failure modes, stress–strain curves and various performance indicators were analyzed to evaluate the bonding mechanical properties and the effects of different factors. The results revealed that for straight-end SMA fibers, increasing the diameter and depth-to-diameter ratio both led to a decrease in bonding strength. On the other hand, the N-shaped end provided sufficient anchorage force for SMA fibers, resulting in a maximum pull-out stress of 926.3 MPa and a fiber strength utilization of over 78%. Increasing the fiber diameter enhanced the maximum pull-out stress and maximum anchorage stress for N-shaped-end SMA fibers but reduced the fiber strength utilization. These research findings provide a solid theoretical basis and data support for achieving a synergistic effect between SMA fibers and the ECC matrix.
An Experimental Approach to Assess the Sensitivity of a Smart Concrete
Authors: Shahzad Shaban , Toumi Ahmed , Balayssac Jean-Paul , Turatsinze Anaclet
Published: August 2023

Structural health monitoring of concrete infrastructure is a critical concern for timely repair and maintenance. This study provides an innovative approach utilizing smart concrete integrated with multi-walled carbon nanotubes (MWCNTs) to enhance electrical conductivity. The smart concrete’s self-sensing capability is assessed through fractional change in electrical resistance (FCR) measured using a four-probe technique. Four-point bending and compressive tests explore the material’s response to cyclic and monotonic loads. Additionally, the impact of saturation levels on self-sensing sensitivity is investigated through compressive tests on varying saturation degrees. Remarkably, a substantial correlation between crack mouth opening displacement (CMOD) and FCR is observed during cyclic bending tests, where FCR increases significantly (from 0.019% to 154%) as CMOD rises from 0.004 mm to 0.55 mm. Digital image correlation (DIC) further validates CMOD measurements and their correlation with FCR. Moreover, this study reveals that amplitude of loading and degree of saturation have a significant effect on the self-sensing of the smart concrete. In saturated conditions, the self-sensing response of the material is insensitive to the mechanical strain, while with reduction in the saturation degree, a quasi-linear response is observed. To assess the sensitivity of the smart concrete, stress and strain sensitivities were evaluated, revealing a noteworthy enhancement of approximately 33% and 50% in stress and strain sensitivity, respectively, as saturation levels decreased. The self-sensing response of the material is very sensitive to the mechanical strain during monotonic loading and damage. These findings indicate the potential of smart concrete as a promising tool for comprehensive, real-time structural health monitoring for infrastructure during its entire life.