bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Settlement Caused by Tunnel Underneath Existing Railway after Construction
Authors: Xu Ze Pei , Li Xi Bing
Published: September 2013

For the existing railway, the settlement after construction caused by the tunnel underneath it is completed after the tunnel has been constructed. It is the result that the stress adjustment and consolidation of soil enhanced itself under the live load of train. Through the settlement can be calculated by peck formula in the stage of and after construction, for the settlement after construction, the peck formula must be transformed with the fitting monitoring curve. With the engineering example, the fitting formula of settlement after construction can be used for analysis and prediction of similar engineering.
Research on the Effect of Grassland Highway Curves on Driver’s HRV
Authors: Zhu Shou Lin , Qi Chun Hua , Gao Ming Xing , Yang Feng
Published: September 2013

Improper design of grasslands highway curve is an important cause of accident, which research on is not mature. Drivers are the main body of grasslands highway traffic man-machine environment system. The paper aims at four sections of highway curve, using frequency domain analysis and grey correlation analysis method to study the relationship between driver’s HFnorm, LH/HF and the highway curves. Results indicate that it can not distinguish the effects of driver's sympathetic nerve, vagus nerve and the balance between them caused by different curves in statistical terms. In the monotonous landscape grasslands, increasing some curves of different radii appropriately in highway alignment design can relieve driver’s fatigue to a certain extent. Diversification of grasslands highway curve has a less influence on parasympathetic nerve of young drivers with long driving experience than that of old drivers with long driving experience. To ensure the driver’s autonomic nerve activity being at a reasonable state, it is more perfect to design grasslands highway horizontal and vertical curves combined with driver’s LF/HF indexes, and profile grade with driver’s HFnorm and LH/HF indexes. The results can offer part of theoretical support for thorough study on humanity of grasslands highway alignment.
Prediction for Sound Radiated Power from Submerged Double Cylindrical Shells Based on Measuring Vibration of Inner Shell
Authors: Zhang Chao , Shang De Jiang , Li Qi
Published: September 2013

A prediction method for the sound radiated power from submerged double cylindrical shells based on measuring vibration of inner shell is presented. The prediction model of submerged double cylindrical shells is established by using modal superposition method. Applied the ratio of the measuring value and theoretical value of the acceleration in one point or mean square velocity of inner shell, and combined with the theoretical value of the sound radiated power, the predicted value of the sound radiated power is derived. The corresponding experiment is carried out in lake. And then the measuring power curve is compared with the predicted power curve based on this method. The result shows that they have good agreement and the average prediction error is less than 2dB.
Simulation Analysis on Wheel and Groove Rail Contact Position
Authors: Chen Rui Ying , Wei Kai , Xu Yu De
Published: September 2013

A vehicle-rail dynamic model is established and the calculated results are verified with trace method. And under the conditions of a same curve section, simulations are made on the contact position of groove rail and standard rail. Variation of contact points and contact area are analyzed. Furthermore, comparisons are made on the two rails with different lateral displacements.
A Professional Safety Consultant Mode for Engineering Construction and its Application in Taizhou Bridge
Authors: Liu Xiao Yong , Zhang Er Qiang , Hu Yi Ming , Han Hui
Published: September 2013

In development countries, safety management of engineering construction is usually undertaken by professional agencies with corresponding safety qualification. But In Chinese Mainland, it is basically implemented by the development organizations own staffs that are lack of enough professional safety management knowledge. A kind of engineering construction safety Consultant mode, with the connation of systematical Management, professional Consultant, on-site Guidance, standardized Operation (thus named "MCGO" safety consultant mode), is put forward in this paper. It means a professional agency is entrusted by the project development organization to carry out the on-site safety management. The implement and operation ways of "MCGO" Safety Consultant Mode are elaborated. This mode has been successfully applied in the safety management of Taizhou Bridge, which was praised by the Ministry of Transport of China as safety management model of extra-large bridge construction.
Special Wetting Behaviors on Canna Leaf and its Dependence with Microstructures
Authors: Yang Xiao Hua , Xiao Jian Hua , Ou Jun Fei
Published: September 2013

Like lotus leaf and rose petal, the canna leaf also has excellent super hydrophobicity.The purpose of this paper is to systematically study the super hydrophilicity of canna leaf. Using SEM to observe the morphology of the canna leaf, and analytical balance to measure the adhensive force between water droplet and the leaf . This paper shows that the first type of the canna leaf which has co-exsitence of the nanocrumb and micro-scale convex cells has the high contact angle and low contact angle hysteresis similar to lotus leaf. The another type on the leaf has high contact angle but high adhesion in a certain extent like the rose petal effect, whose microstructure unitarily simple has the micro convex cells, do not distributed anything of nanoscale.
The Processing Technology and Dynamic Balancing of Turbocharger Rotor Parts
Authors: Gan Hui
Published: September 2013

The passage analyzed the influence factors of dynamic balance of the turbocharger rotor. According to the structure of turbo rotor components and compressor impeller, the article proposes the matching process of impeller shaft and the selection of equipment model and expounds the importance of rotor dynamic balance. The working principles of two models of whole dynamic balancing machine are comparative analyzed, so as to provide a selection for processing choice.
Rail Profile Wear on Curve and its Effect on Wheel-Rail Contact Geometry
Authors: Wei Kai , Chen Rui Ying , Xu Yu De
Published: September 2013

The paper has carried out continued tests on a curve of a heavy haul railway in China for its rail profiles. Based on the data, the paper has counted the development of the rail profile wear, and then analyses the influence of wheel-rail contact geometry on the rail profile wear. The results show that the wear of high rails develops around the rail corners, while the one of low rails around the rail top. The development of the rail wear speeds up after the transport mass passes 210MGT. The wheel-rail contact geometry deteriorates when the transport mass grows up to 60MGT and lower than 210MGT.
Bidirectional Dynamic Programming Approach for Highway Alignment Optimization
Authors: Li Wei , Pu Hao , Zhao Hai Feng , Liu Wei
Published: September 2013

In order to automatically generate the optimal highway alignment and avoid omitting valuable scenarios, a bidirectional dynamic programming approach for alignment optimization was put forward. Firstly we established a three-dimensional search grid in the study area. Then dynamic programming was used to find the best alignments within the vast study area. A double-direction searching strategy was proposed to obtain a group of scenarios ranking by their corresponding comprehensive cost. The alignments were then refined with Powell method so that the resulting alignments could deviate from the grid points to reach better solutions. Examples demonstrated that this approach can obtain global optimal alignment with good diversity.
Study on the Ignition Factor of Piston Kerosene Engine
Authors: Li Shi Hong , Jin Yan , Ni Kong Shi
Published: September 2013

After the fuel changed fromgasoline to kerosene of internal combustion engine, the engine cold startperformance is poor due to the poor spray evaporation of kerosene fuel. In thepaper, the ignition factors are to be analyzed based on the gas dischargetheory. Improving the spark plug discharge and increasing the ignition energyare used to improve the cold start performance of engine. The experimentalresults show that the method can improve the cold start performance of engine,which provides the basis for the engine burning kerosene fuel
Simulation and Optimization of Turbine Blade Shape Modification
Authors: He Fang
Published: September 2013

Thickness thinning repair analog simulated using fluid mechanics research tool ANSYS-CFX of mixed-flow turbine runner blade,Through the three-dimensional flow and numerical calculation and analysis of energy distribution on the blade after featheredging. Leaf blade repair thickness thinning after 0.5CM in the cavitation performance, vibration performance and operation performance of the performance indicators to achieve the ideal state, The new method for reducing wheel vibration, vortex row, There is a certain positive significance in improving the efficiency of turbine, make full use of new energy.
Application of Grey-Markov Model in Predicting Container Throughput of Fujian Province
Authors: Chen Cai Ping , Liu Qiao Jing , Zheng Pan
Published: September 2013

Port container throughput forecast is an important work for wharf project which has further influence on the development of ports. In this paper, we simply introduce the grey system theory and the grey markov model. Further more, we make a forecast for the container throughput by using grey markov model and the result can offer the decision-making bases for relevant department.
The Long Highway Tunnel Disaster Prevention Research
Authors: Li Zhi Wei , Xi Bao Ping , Guo Qing Hua , Tian Jun Bin
Published: September 2013

This study takes the long highway as the object, discuss generally fire, ventilation, lighting, tunnel geological and other key technical issues, and analyze the importance of these disasters' prevention and control. Via the study of the tunnel fires' characteristics and damages, this thesis puts forward some views on the construction of fire access doors and maintenance, and works out the corresponding fire prevention measures.
Tunnel Waterproofing Board Construction Process Analysis
Authors: Ke Dong Qing , Zhong Xiang Xun , Guo Dong Hua
Published: September 2013

This article from preparation befor the job, blind pipe with fixed and waterproof plate laying geotextile, analysis of waterproof board laid before the preparatory work, waterproof board laid process related indicators of control, and offer reference for the construction of the tunnel.
Research a New Automatic Detective Method of Highway Traffic Statement Based on the Information From the License Plate Data
Authors: Zhang Huan Jiong , Jin Xiao Ping , Zhang Ping
Published: September 2013

in this paper, the key technology of the traffic statement automatic detection is researched, based on analyzing the properties of the information from the collected license plates, a new automatic traffic statement detective method is pointed out, this new method can express the essential characters of the traffic statement, and valuably gain the precise degree. This automatic detective method expresses the fine applicative prospect in the field of the real time traffic statement detection and management.
Effects of Casting Temperature and RE Refiner on Microstructure of the Squeeze-Cast Al-Zn-Mg-Cu Alloy Drive Hollow Shaft
Authors: Fan Cai He , Zhu Yue Bing , Yang Na
Published: September 2013

Effects of casting temperature and RE refiner on microstructure of the hollow Al-Zn-Mg-Cu alloy drive shaft prepared by squeeze casting ( in express locomotive) were investigated in this paper. As the casting temperature increase from 720°C up to 780°C, the average grain size of the α-A1 primary grains increased from 32μm up to 51μm, and the average equivalent roundness changed irregularly. Meanwhile the variation range was not big and the roundness value ranged from 0.63 to 0.72. With the casting temperature up to 780°C, the average equivalent roundness was 0.71. When the refiner was adopted, the average grain size of the alloy decreased obviously. However, no obvious change was detected in the average equivalent roundness, which ranged from 0.73 to 0.78. With the refiner of 3wt% Al-10RE, the average grain size of the alloy and the average equivalent roundness were 35μm and 0.77, respectively.
MOTION - A Developing Urban Adaptive Traffic Signal Control System
Authors: Jiang Peng Peng , Poschinger Andreas , Qi Tong Yan
Published: September 2013

This paper mainly focuses on the recent development of MOTION system. MOTION system, as a developing urban adaptive traffic signal control system, is now more and more worldwide recognized. In this paper, first the original idea of MOTION is descript. Calculation procedures of MOTION algorithm are followed. Then methods of optimization, including optimization of green split, optimization of cycle time, optimization of offset times are introduced. Platoon Model, as a vital concept in optimization of offset times, are explained in details. Multi functional levels in MOTION system including the tactical level and the operational level are introduced in figures. At last, implementation results of MOTION system in Germany are shown to prove MOTION as a very efficient method in improving urban traffic quality.
Real-Time Monitoring School Buses Safety and Information Query System Design
Authors: Jin Miao Miao , Liu Chang Chun , Hai Chuang
Published: September 2013

The management of school buss safety and information system: achieving real-time monitoring of the school bus by making use of the IC card swiping and counting, GPS positioning, speed limiter, infrared sensors and other technologies. Information related to school bus driving collected from the vehicle terminal is sent to the network platform where parents can inquire the real-time information. Consequently, it keeps close contact between parents, schools, and students as well as fully protects the safety of school buses.
Valve Cam Profile Design of High-Speed Diesel Engine Based on MATLAB
Authors: Li Yan , Ren Ping Li , Liu Shao Hua
Published: September 2013

Through MATLAB programming, the parameters of valve cam profile will be selected and calculated. The general design principles and methods for higher-degree polynomial asymmetrical cam profile will be introduced, improved design and experimental verification have been conducted to the cam profile of 4C18 diesel engine, and significant result has been obtained. The result shows that the higher-degree polynomial asymmetrical cam profile can effectively improve the dynamic property of valve cam and the economy, emission and dynamic property of the high-speed working section of the engine.
Research on the Modeling Methods of the Service Network Meeting the Requirements of Passenger Flow Assignment in Urban Rail Transit
Authors: Yu Jian , Zhang Xing Chen , Xu Bin
Published: September 2013

The conceptual introduction of the service network and its function in passenger flow assignment are given. Based on the summary of the related research results, the service networks are divided into two classes as the matrix type and the network type according to the different construction methods. The modeling principles and methods of the network type service network are then proposed. The methods are explained in three aspects including node modeling, segment (or arc) modeling, and partial network modeling, which can, for a certain physical network and a certain line plan, construct the virtual service network that is required by the passenger flow assignment process. Corresponding modifications are required when using these methods in specific researches.
A Study on Mathematical Model of Servo Valve Torque Motor
Authors: Liu Chang Hai , Jiang Hong Zhou
Published: September 2013

In this paper, a comprehensive theoretical analysis of servo valve torque motor that includes the ignored magnetic reluctance of magnetic elements is carried out. These analyses are based on fundamental laws of electromagnetism and general mechanics, thus a new mathematical model of servo valve torque motor is derived to improve the accuracy of the model. When using this model structure to fit the simulation data of electromagnetic torque constant and magnetic spring stiffness, the result render closer agreement with than when using existing models.
The Research and Development of Filtration Media Materials for Application in Water Filtration Treatment Systems
Authors: Zhang Bao Li , Lu Jing , Wu Wen Liang , Xie Ying Si , Sheng Yi Zhi
Published: September 2013

This paper introduced the vary application filtration treatment systems, different grades of water and several media materials that could be applied in infiltration treatment systems, along with characteristic of these media materials. The characterization and analyses of the media and their effects on the removal of specific contaminants, and factors affecting the fate and behavior of various contaminants in filtration systems were discussed. Case studies of the appropriate application of infiltration treatment systems were also included.
Analysis on Traffic Characteristics of Passenger Cars with more than 7 Seats on Megacity's Freeway Network Using ETC Data
Authors: Zhao Na Le , Jiang Peng Peng , Qi Tong Yan
Published: September 2013

The traffic characteristics of passenger cars with more than 7 seats are directly related to the operation status of megacitys freeway network. With applying 3-months ETC data, this paper aims to propose methods for traffic characteristics analysis of passenger cars with more than 7 seats. Moreover, based on the macroscopic indexes calculation such as travels per day, penetration of local vehicles, average speed and average driving distance, the traffic characteristics of these vehicles are analyzed respectively in holidays, weekends and working days.
Study on Tension Model of Running Deviation for Strip Rolling
Authors: Zheng Shen Bai , Huo Xin Zhou , Yin Jin Zhi , Yang Gai Yan , Zhang Wei
Published: September 2013

For running deviation of strip and camber deviation, a tension model was established on the discussion of the dynamic equilibrium state under tension. The friction difference of strips sides or workpiece wedge causes plain rolling camber, meanwhile housings elasticity inequality or roll tilt leads to axial shifting and camber deviation. In the digital simulation, the tension-drawing model is necessary to realize tension self-adjusting, and in the production, tension increments can be calculated for adjusting the two sides gaps.
Study on the Berthing Capability of Pontoon Bases
Authors: Xie Yao Feng , Liu Xiao Xi , Tang Jie Ping
Published: September 2013

The Xinshengwei base of Nanjing port is located in the water area of the Yangtse River. To meet the development of the port, the owners decide to rebuild and level up the Xinshengwei base to increase the transit quantity of integrated barges. A research on the working conditions after the base is rebuilt and promoted is of great necessity. By analyzing the working conditions the base required, the main effect factors, the working properties of the berthing capability and the anchor-chain system in detail systematically, an integrated numerical method for berthing capability is proposed. Computing impact force when berthing in work-energy theory overcomes the defect of the method based on experience. The upper limit controlled condition is raised through the research. The research results fit the in-situ mooring test well.
The Impact of Plateau Airport's Environment on Air Traffic Controller's Situation Awareness
Authors: Pan Wei Jun , Wu Dan , Lu Na
Published: September 2013

The operation of plateau airport is the major problem for the development of China civil aviation transportation. Situation awareness (SA) research is of great importance to the improvement of air traffic controllers training and air traffic safety of plateau airport. First of all, the concept of SA as well as SA assessment methods was introduced in this paper. According to the summation of plateau airport situation, the impact of exposure to plateau environment on controllers SA was analyzed in details. Finally, it reveals what the trend of research on air traffic controllers SA is and what the core problems to be solved are.
Study on Driver’s Reaction Time of Emergency Braking
Authors: Zhao Gui Fan , Wang Shen Si , Yang Na , Qing Ke , Liu Ming Min
Published: September 2013

From the view of driver’s driving behavior and reaction characteristics, the factors affecting driver’s reaction time are comprehensively analyzed. Then design and prepare driver’s reaction time of emergency braking detection system on both hardware and software by using MCU. And according to the factors affecting driver’s reaction time, the reaction time of emergency braking of different gender drivers in different conditions is tested by using orthogonal test method. From the experimental results, the degree of influence of different factors and different levels on driver’s reaction time is obtained and this provides a reference for safety driving.
Study on the Length of Speed-Change Lane in Freeway Trumpet Interchange
Authors: Hu Jiang Bi , Ma Wen Qian , Wang Meng
Published: September 2013

Nowadays, speed-change lane of freeway interchange is a big safety risks area. The reasonable length of speed-change lane can effectively reduce the safety risk. From the point of drivers safety and comfort, this paper studies on the length of speed-change lane by doing experiment on drivers driving on behalf of cars and trucks. Through the theoretical and experimental analysis, we study on vehicle speed and variation of driver driving workload, establishing the relationship between length and drivers safety and comfort. Under the conditions of the design speed of 120km/h, the general values and limit values of length which meet the drivers expectation are provided. They are compared with the current specification index in China. This paper puts forward a new idea and method to study on the length design index standard of speed-change lane in freeway trumpet interchange.
Study on Unsteady Temperature Field of Diesel Engine Piston
Authors: Liu You , Liu Jing Jing , Chen Xiao
Published: September 2013

The paper took the piston of 620 single-cylinder engine as an example,a finite element model of piston was built with the help of ANSYS software. Than to calculate temperature field and and quick cold start conditions respectively[1]. Through the periodic transient thermal analysis, temperature fluctuations on the surface of the piston were derived, which indicated that the surface contacted with gas was the main temperature fluctuation area. The max temperature fluctuation can be up to 20 ° C and the wild fluctuation occurred in a distance of 2 mm from the surface of the piston. Temperatures of the piston went up according to exponential rule during the course of quick cold start, Extreme temperature fluctuations will generate huge quasi-static thermal stress.
Research on Drop and Pull Transportation Cost of Entire Car
Authors: Zhang Li Wei , Zhu Min Jie , Li Gui Jun
Published: September 2013

We try to provide the quality logistics services to customers at the lowest logistics cost,by the model of drop and pull transportation of the entire car. To this end, this paper carried on the economic benefit analysis and comparison from two aspects of the fixed cost and variable cost between the drop and pull transportation and the traditional transportation model for the entire car.
Keywords: the Entire Car Transportation, the Drop and Pull Transportation, the Traditional Transportation, the Logistics Cost, the Analysis and Comparison
Synthesis and Characterization of 7 - (3 - (substituted-phenoxy) propoxy) Quinazoline Derivatives
Authors: Yan He Ping , Ouyang Gui Ping
Published: September 2013

Four compounds of 7-(3- (substituted-phenoxy) propoxy) quinazoline compounds, including 7-(3-(2,4-dichlorophenoxy) propoxy)-N-(3-chlorophenyl)-6-methoxyquinazolin-4-amine, 7-(3-(2-chlo-rophenoxy) propoxy)-N-(3-chlorophenyl)-6-methoxyquinazolin-4-amine, 7-(3-(4-chlorophenoxy) prop-oxy)-N-(3-chlorophenyl)-6-methoxyquinazolin-4-amine, 7-(3-(naphthalen-3-yloxy) propoxy)-N-(3-chl-orophenyl)-6-methoxyquinazolin-4-amine, were synthesized fromN-(5-(3-chloropropoxy)-2-cyano-4-methoxyphen-yl)-N, N-dimethylformamidine by cyclization,etheration, in the yield of 45.9%50.6%56.34% and 80.6% respectively. Their structures were characterized by IR,1H NMR,13C NMR, MS and elemental analysis.
Comparison of Growth Kinetic of Urinary Crystallites from Patients with Calcium Oxalate Stones with that from Healthy Subjects
Authors: Shang Yun Feng , Kuan Li , Xue Jun Fa , Ouyang Jian Ming
Published: September 2013

The differences of growth kinetic of urinary crystallites from patients with CaOxa stones and healthy subjects were compared. With the increase of crystal growth time (t), the size of urinary crystallites from patients increased constantly from 10±9 μm att=1 h to 50±45 μm att=72 h, but the number of urinary crystallites decreased gradually from 1820±610 ind./mm2att=1 h to 220±98 ind./mm2att=72 h, indicating that the formation process of crystallites in lithogenic urine was ascribed to growth control. In contrast, for healthy subjects, the number of crystallites increased from 1650±850 ind./mm2att=1 h to 1800±830 ind./mm2att=72 h. However, the particle size was slowly increased from 7±5 μm att=1 h to 14±13 μm att=72 h, while the sizes of most urinary crystallites were still less than 20 μm, indicating that the growth process of crystallites in healthy urine was dominated by nucleation control. The differences mentioned above are mainly attributed to that both the concentration and the activity of the inhibitors in healthy urine were higher than those in lithogenic urine, and the inhibitors in healthy urine can inhibit the growth and aggregation of urinary crystallites more effectively. This result can help to elucidate the renal-calculi formation mechanism.
Electrospray Ionization Mass Spectral Characteristics and Fragmentation Mechanisms of Tanshinones in Radix Salvia miltiorrhiza
Authors: Liu Tao , Zhang Gui Feng , Yin Hui , Jin Xiao Bao , Zhu Jia Yong
Published: September 2013

Cryptotanshinone, tanshinone I, and tanshinone IIA were analyzed by High-performance liquid chromatography /electrospray ionization-mass spectrometry. MSn spectra were obtained and optimized by energy collision induced dissociation (CID) from [M+H]+ ions, the effect of collision energy on production of fragmental ions were studied and optimal signals were achieved. With the collision energy of 35%, tanshinones have optimal signals of fragmental ions and maximal amounts of product fragment ions respectively. The fragmentation pathways for the compounds were studied, and this information would be helpful for the quantitative and pharmacokinetic analysis of tanshinones.
Preparation, Characterization Evaluation of Tanshinone IIA Lipid Microspere and Comparative Study of Pharmacokinetics with Sodium Tanshinone IIA Sulfonate Injection
Authors: Liang Xin Li , Zhu Meng Liang , Cao Yun Chao , Zhao Guo Wei , Jiang Qie Ying , Zhao Li Jun , Liao Zheng Gen
Published: September 2013

In this study, Tanshinone IIA lipid microspere (Tan-IIA-LM) was successfully prepared by an ultrasonic and high-pressure homogenization method. The particle size, zeta potential, drug entrapment efficiency and morphological properties of Tan-IIA-LM were characterized in detail. A pharmacokinetic comparative study of Tan-IIA-LM with Sodium Tanshinone IIA Silate (STS) injection was developed. A sensitive and specific HPLC method was established to determine the mass concentration of Tan-IIA and STS in rats plasma samples which were compared between Tan-IIA-LM and STS injection after single dose intravenous administration of 18.35μmol/kg and the results were fitted and analyzed by dedicated software. After intravenous administration, the AUC0-and Cmaxvalues of Tan-IIA were 2.14, 2.22 fold higher than STS. The clearance, apparent volume of distribution and mean residence time (MRT) of Tan-IIA were lower (P<0.01) than STS; other pharmacokinetic parameters had no significant deviation (P>0.05). The pharmacokinetic of Tan-IIA, STS have significant differences at the same molar doses and the AUC and Cmaxvalues of Tan-IIA were higher compared with STS.
High Expression and Preliminary Purification of Human β-Defensin-2 Fusion Protein
Authors: Wei Hong Tao , Lv Zhong Wen , Han Xue Mei , Zhang Guo Li
Published: September 2013

This study was undertaken to achieve high expression and preliminary purification of human β-defensin-2 fusion protein to lay a solid foundation for production of human β-defensin-2 using genetic engineering. A prokaryotic expression vector for human β-defensin-2 fusion protein was generated using in vitro gene synthesis before transformation into BL21 (l DE3) plysS TrX-B host bacteria. High expression of TrX-A-HBD-2 fusion protein was induced with IPTG in the bacteria exposed to various expression conditions. The fusion protein then underwent preliminary purification. The protein of interest was released from the genetically engineered bacteria after freezing and thawing. The expression of the target protein accounted for 16.12% of the total bacterial proteins. Fractional precipitation with saturated ammonium sulfate and metal chelate affinity chromatography yielded human β-defensin-2 peptide fusion protein, with a relative purity of 80.53%.Human β-defensin-2 fusion protein could be highly expressed in a soluble form, with a relatively high purity
Anticancer Activity of Novel Platinum Complex as DNA Binders
Authors: Luo Xu Jian , Qin Qi Pin , Li Yu Lan , Liu Yan Cheng
Published: September 2013

A new phenanthroimidazole platinum (II) complex has been synthesized and characterized by IR, NMR, ESI-MS, element analysis. The affinities of the complex toward ct-DNA was determined by circular dichroism absorption (CD), UV-Vis absorption. Results indicate that the complex interact with ct-DNA by classical intercalating mode. The cytotoxicities of the complex was screened against four cancer cell lines and normal cells of HL-7702 in comparison to cisplatin and it showed a higher activity than cisplatin, with IC50 values in the range 8.7417.11 μmol/L. Furthermore, the complex displayed lower cytotoxic activities to HL-7702 (normal cell) compared with the cancer cell lines.
Anticancer Activity of Platinum (II) Complex as G-Quadruplex DNA Binders
Authors: Qin Qi Pin , Li Yu Lan , Liu Yan Cheng , Luo Xu Jian
Published: September 2013

A platinum (II) complex has been synthesized and characterized. The complex binding properties with G-quadruplex DNA and ds26 were examined by FID and CD spectroscopic methods. The results revealed that the platinum (II) complex can induce the antiparallel G-quadruplex structure of HTG21 conformation in the absence of added K+ with selectivity over other G-quadruplex DNA and duplex DNA. The cytotoxicity of the platinum (II) was screened against four cancer cell lines and normal cells of HL-7702 in comparison to cisplatin and it showed a higher activity than cisplatin, with inhibition rates ranging from (40.06±1.65)% to (89.47±1.14)%. Furthermore, the platinum (II) complex displayed lower cytotoxic activities to HL-7702 (normal cell) compared with the cancer cell lines.
Inhibitory Effects of Natural Food Dyes Genistein on Invasion of SKOV3 Human Ovarian Carcinoma Cells In Vivo and In Vitro
Authors: Li Tang , Yu Li
Published: September 2013

The invasion behavior of tumor cells is important in the metastasis process of ovarian cancer cells. In this study we investigated the effects of Genistein on invasion inhibition in ovarian carcinoma cell line SKOV3 in vivo and in vitro. The abilities of the Genistein-treated SKOV3 cells to invade through reconstitute matrigel in transwell chambers were investigated in vitro and the invasion effect in vivo was determined by using the xenograft models of SKOV3 in nude mice. The ability of the 20μmol/L Genistein-treated cells to invade the reconstitute basement membrane was decreased significantly at 72h. This inhibition was dose-dependent. 40μmol/L Genistein had the strongest effect. The in vivo result suggested that the grade of invasion in control SKOV3 cells was time-dependent and Genistein-treated group could apparently inhibit the progress of invasion, localizing the tumor in invasion Grade 0 or Grade I and decreasing the proportion of Grade II, III and IV. The results suggested that Genistein possessed inhibitory effect on invasion in human ovarian carcinoma cell lines SKOV3 in vivo and in vitro.
The Effect of Plant Polyphenol Genistein on Migration of Endothelial Cells Induced by Human Ovarian Carcinoma Cell Line SKOV3
Authors: Tang Li , Yu Li
Published: September 2013

Angiogenesis is crucial for tumor progression and and migration of endothelial cells into tumor is important in the formation of new blood vessels in tumors. In this study we investigated the effect of Genistein on migration of human endothelial cells ECV-304 induced by human ovarian serous cystadenocarcinoma cell line SKOV3, and explored the mechanism of anti-angiogenesis of Genistein. Millicell chamber and coculture method were used to observe the influence on migration of ECV-304 induced by SKOV3 cells or its conditioned medium. The expression of angiogenesis associated protein VEGFbFGF and TGFβ-1 were determined using immunocytochemical assay. The results showed that either SKOV3 cells or its conditioned medium could induce the committed chemotactic migration of ECV-304. The chemotactic migrations of ECV-304 induced by SKOV3 or its conditioned medium were significantly inhibited by Genistein in a dose-dependent manner. 20μmol/L Genistein could down-regulate the expression of bFGF, and up-regulate the expression of TGFβ-1. Migrations of ECV-304 induced by SKOV3 or its conditioned medium are apparently inhibited by Genistein. It suggests that this inhibitory effect of Genistein is completed by down-regulating the expression of vessel growth-promoting factor bFGF, and up-regulating the expression of negative regulator TGFβ-1. This may be one of the mechanisms of anti-angiogenesis of Genistein.
The Regulation of Natural Food Dyes Curcumin on the Amyloidogenic Pathway of APP
Authors: Zhang Xiong , Sun Jie Yun , Zhang Hong Mei , Si Lu , Li Yu
Published: September 2013

As a promising prevention and therapeutic intervention in Alzheimer’s disease, natural food dyes curcumin could obviously inhibit the generation of Aβ, but the mechanism is not fully defined. This study aims to investigate the effects of curcumin on the amyloidogenic pathway of APP in vitro. Plasmids APPswe and BACE1-mychis were transiently co-transfected into SH-SY5Y and HEK293 cells. Then, they were treated with curcumin at 0, 1.25, 5, 20 μmol/L for 24 h, or with curcumin at 5μmol/L for 0, 12, 24 and 48 h for the time course assay. Our findings showed that curcumin could inhibit the expression of the APP at mRNA level; the expression of the BACE1 and C99 at mRNA and protein levels, furthermore it could inhibit the generation of Aβ40/42, and those changes were dose-time dependent (p<0.05). Our study indicates that Aβ40/42 generation inhibition effect of curcumin might due to its influence on amyloidogenic pathway. This may provide important experimental basis for AD treatment with curcumin.
Optimization of the Water Extraction Technology of Flavonoids in Thlaspi arvense Linn
Authors: Ge Yan , Ma Rong Kai , Wang Li Sheng , Li Xuan Hai , Luo Xuan
Published: September 2013

Single factor experiments and orthogonal experiments were successively carried out to optimize the water extraction technology of flavonoids in Thlaspi arvense Linn. Results showed that the influential order of factors was extraction times >solid-liquid ratio >extraction time. The optimum conditions were adding 16 times amount of water, extracting 3 times and every time with 4 hours. Under these conditions, the yield of flavonoids from Thlaspi arvense Linn was 1.17%.
Preparation and Characterization of Levalbuterol Hydrochloride Drug-Resin
Authors: Yu Chu Qin , Zhang Kun Shui , Lin Hua Qing , Lin Su Na , Luo Xiu Zhen , Lin Di Shi
Published: September 2013

In this study, our objective was to prepare levalbuterol hydrochloride drug-resin and investigate its characteristics. This work is basis for the study of the next levalbuterol hydrochloride sustained-release suspensions. The drug-resin was prepared by bath method. The effect of factors , including resin types, drug concentration, speed, temperatures and pH on evaluation index, was evaluate by Drug utilization rate and the resin loading. Then the Box-Behnken Design-response surface method was used to optimize the preparation. The drug content determined by UV method and drug-resin were characterize by IR and DSC．The best formulation of levalbuterol hydrochloride drug-resin was used the bath method IRP69 resin at 30°C. The drug concentration was 1.8 mg•ml-1, and the rotate speed was 250 rpm, the pH was 5.8. The preparation of levalbuterol hydrochloride drug-resin method is simple and feasible, and the drug combined with the resin by the chemisorption of ionic bond.
Design, Synthesis and Biological Evaluation of Novel Anticancer Agent Sprio Indolone Compounds
Authors: Li Jing Yu , Zhang Xiao Min , Song Bin Bin , Yu Peng , Lu Kui
Published: September 2013

Indolone is a kind of natural product which was widely existed in the animal bodies and plants, which possess varieties of biological activities, such as anti-bacterial, anti-tumor, anti-aging and anti-anxiety activities. As we all known that many sprio compounds which containing hetero atom such as O, N, S have good biological activities. In this paper, a series of spiro indolone derivatives was design with the combination of indolone and sprio scaffold, 6 novel target compounds were synthesized in 3 or 4 steps in 6%-17% overall yield, which were characterized by1H NMR and their biological activities on tumor cells growth inhibition on human hepatoma cell HePG2 and human leukemia cell K562 by MTT method were evaluated.
Influence of pH on Hydroxyl Radical Scavenging Ratio by Gingerol
Authors: Li Cheng Shuai , Du Ai Ling , Du Ai Qin
Published: September 2013

In this paper, the work was mainly studying influence of pH on Fenton reaction and gingerol scavenging hydroxyl radical. The antioxidant activity of gingerol was characterized by the scavenging hydroxyl ratio and scavenging hydroxyl ratio was analyzed by the spectrophotometry. Hydroxyl radical was produced by Fenton reaction. In the experiment pH and buffer solution have influence on the scavenging hydroxyl radical and Fenton reaction. Gingerol exhibits a strong scavenging radical activity for hydroxyl radical (OH.). The scavenging hydroxyl radical of different extractions of ginger oleoresin is different.
Safety and Management of Food Additives in the United States
Authors: Xu Bo
Published: September 2013

Food safety is an important issue related to the government regulatory authorities, food industry and food consumers. And the increasing use of food additives has become a matter of public and administrative concern, so an extensive safety evaluation on food additives must be carried out and the use of the additives in food should be controlled by law. In the United States, the Congress has entrusted the FDA with the responsibility to ensure that new additives to be used in foods and the foods the consumers purchase are safe. This paper discusses the supervision and management system of food additives in the United States. The conclusion is that FDA has developed a scientifically rigorous, sound and dependable system to assure the safety of food, thus a new food additive must be approved by FDA before it can be used in food. Management of food additives in the United States is also a helpful reference for government food control agencies in other countries.
Characterization of Listeria monocytogenes on Retail Food from Jiangsu Province, China
Authors: Yu Tao
Published: September 2013

Listeria monocytogenes isolates recovered from retail foods were characterized by serotyping, genotyping using pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Six serotypes were identified among the isolates and the predominant serotype was 1/2a (42, 48.8%). PFGE differentiated the 86 isolates into 23 ApaI restriction patterns. Twenty-five (29.1%) of the L.monocytogenes strains exhibited resistance to at least one antimicrobial. Resistance to ciprofloxacin (17.4% of the isolates were resistant) and resistance to tetracycline (17.4%) were observed frequently.
Chiral Discrimination of the Diastereomeric Salts with (R)-Nipecotic Acid and Tartaric Acid Derivative
Authors: Sun Xiao Xia , Li Ying Chun , Zhuang Xiao Xiao , Hu Yu
Published: September 2013

An effective resolving agent, (D)-dibenzoyl tartaric acid (2b), was screened to yield direct resolution of (S)- ethyl nipecotate (1) with high optical purity and yield. Molecular recognition was studied by 1H NMR, IR, DSC and single crystal diffraction of the pair of diastereomeric salts. The less-soluble salt formed a supra molecular structure by enantio differentiating self-assembly.
Application and Development of Molecularly Imprinted Polymer to Solid Phase Extraction
Authors: Jin Yin Zhe , Jin Yun Zhe , Piao Ji Yu , Jin Ying Shan , Xuan Yong Hao
Published: September 2013

Recently, molecularly imprinted solid phase extraction (MISPE) has been used more frequently separate drugs and natural substances. This modern separation methodologies require reliable tools that perform on a high level in terms of efficiency and reproducibility. The molecular imprinting technique is a reliable technique for the preparation of materials of predetermined selectivity. It is highly significant in research of the structure of enzyme, mechanism of receptor-antibody and analytical chemistry. Solid phase extraction can be used to isolate and pre-concentrate the analytes in complex samples. This technique is more rapid, simple, economical and environment-friendly than the traditional liquid-liquid extraction. The materials used in SPE are usually based on the non-specific binding of the targets, which often suffers some shortcomings, such as low specificity and selectivity. In recent years, solid phase extraction involving molecular imprinted polymer have been proved to be successful applications for its features of high selectivity, ease of synthesis, low cost for preparation and workability under different conditions especially that of harsh pH and organic solvents. In this work, the principles, application and development tends of MISPE will be reviewed and the disadvantages and limitation of the MISPE and future development direction are also briefly discussed.
Determination of Imidacloprid, Carbendazim and Thiabendazole Residues in Vegetables and Fruits by HPLC
Authors: Lin Ling , Peng Zheng , Yang Chun Liang , Wang Ming Yue , Zha Yu Bing , Liu Li Li , Zeng Shao Dong
Published: September 2013

The aim of this work was to establish a method that the residues of imidacloprid,carben-dazim and thiabendazole in fruits and vegetables were determined by HPLC.Acetonitrile was edded in the sample for extraction solvent, and using sodium chloride for absorbing water of the sample,then purified by NH2 solid phase extraction cartridge, the sample was detected by HPLC.Under the selected conditions,the detection limits of imidacloprid, carbendazim, thiabendazole were 0.002 mg/kg,0.003 mg/kg and 0.003 mg/kg respectively.Tests for recovery were made by addition of three pesticidess standards at three different concentration levels (0.1, 0.5 and 1.0 mg/kg) to the blank sample.The mean recovery rates of imidacloprid, carbendazimand thiabendazole were87.0%~99.2%,82.0%~96.0% and 80.0%~95.0% respectively , and the relative standard deviations were 0.52%~6.80%.The sensitivity, accuracy and precision of this method were able to meet the requirements for pesticide residue analysis.
Comparative Analysis of Volatile Compounds between Jackfruit (Artocarpus heterophyllus L.) Peel and its Pulp
Authors: Peng Shao Dan , Lin Li Jing , Ouyang Le Jun , Zhu Bing Qing , Yuan Yuan , Jing Wei , Li Ji Hua
Published: September 2013

In the study, the solid-phase microextraction (SPME) and gas chromatography and mass spectrometry (GC-MS) were used for identifying and analysising the volatile compounds of jackfruit (Artocarpus heterophyllus L.) fresh peel (FE) and fresh pulp (FU). The result showed that it was apparent differences of the species and quantities of volatile compounds between peel and pulp. 2,3-Butanediol, Butyrolactone, 1-Methoxy-2-propyl acetate, Butanoic acid, 3-methyl-, butyl ester, n-Amyl isovalerate, n-Hexadecanoic acid were found in peel and pulp. While compounds, such as Geranylgeraniol, Lanosterol, Pentanoic acid, 3-phenylpropyl ester, Phthalic acid, butyl 3-methylbut-2-en-1-yl ester, Hexadecanoic acid, methyl ester, were only present in the peel. But in the pulp, compounds, like Dibutyl phthalate Phenol, 2,4-bis (1,1-dimethylethyl)- henols were confirmed, which did not be found in the peel. The experimental result also indicated that SPME-GCMS is a simple, rapid, reliable and effective method to analyze the volatile fraction in jackfruit. It may have as profound an impact on analysising the volatile compounds of jackfruit.
Optimization for Production Process Conditions of High Temperature Frying Potato Chips
Authors: Liu Yi , Yuan Yue Hua , Liu Yu Feng
Published: September 2013

The production conditions have great influence on nutrition, color, flavor and evaluation of food safety of high temperature (180 °C ~ 190 °C) frying potato chips. The effects of potato slice thickness, total time of drying, frying time, the mixing of antioxidant and synergistic agent and additive on potato chips quality were studied according to the determination of water content, oil content, peroxide value, carbonyl value, acrylamide content and sensory score. The optimum process conditions are show as follows: the slice thickness is 1.0~1.5mm, the drying time is 45~55min, the frying time is 2.0min, the addition mass fraction of antioxidant BHT is 0.02%, the addition mass fraction of synergist citric acid is 0.05% and 0.3% CaCl2 should be added to restrain produce of acrylamide.
The Nutrition of Buffalo Milk: A Comparison with Cow Milk
Authors: Yang Tong Xiang , Wang Fang , Li Hong , Liu Qing Su , Li Quan Yang
Published: September 2013

Milk is the essential source of nutrition for young mammals before they are able to digest other types of food. To explore the complex nutritive value of buffalo milk, the general composition, buffer capacity, the properties of buffalo yogurt were analyzed, meanwhile, cow milk as a control group was also studied. The results show that the nutritional value of buffalo milk is higher than that of cow milk, exhibiting the high protein, fat, lactose, total solids and nonfat solids contents, and the high buffer capacity. Yogurt with the probiotics characterized by acidification activity, syneresis and water-holding capacity, has the functional of lactobacillus resisting lactose intolerance and reducing constipation. Buffalo yogurt shows the higher stability and nutritional value.
Hydrothermal Synthesis Hollow BiVO4 Photocatalysts
Authors: Cao Bao Wei , Xu Yun Hua
Published: September 2013

Hollow BiVO4 microspheres composed of nanosized particles were successfully prepared via a simple hydrothermal reaction route at 170C for 12 h. The crystal structures, morphologies, and optical properties of the as-prepared samples were characterized by X-ray powder diffraction (XRD), transmission electron microscope (TEM), scanning electron microscopy (SEM) and UV-visible absorption spectra (UV-vis). Simulated Sun-light induced photocatalytic degradation of Rhodamine B by hollow BiVO4 microspheres has been investigated, and the results show the hollow structures with excellent photocatalytic activity.