bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel insights into the anatomy and histopathology of the sacroiliac joint and correlations with imaging signs of sacroiliitis in case of axial spondyloarthritis
Authors: Prati Clément , Lequerre Thierry , Le Goff Benoît , Cortet Bernard , Toumi Hechmi , Tournadre Anne , Marotte Hubert , Lespessailles Eric
Published: May 2023

For a better understanding of the pathophysiology of spondyloarthropathy (SpA), a detailed anatomical description of the sacroiliac joint is required because sacroiliitis is the earliest and most common sign of SpA and an essential feature for the diagnosis of ankylosing spondylitis. Beyond the anatomy, the histopathology of sacroiliac entheses and immunological mechanisms involved in sacroiliitis are crucial for a better understanding of disease causation. In this narrative review, we discuss the core anatomical, histological, and immunohistological observations involved in the development of sacroiliitis, focusing particularly on imaging-based information associated with sacroiliitis. Finally, we try to answer the question of whether at the sacroiliac joint, enthesitis precedes synovitis and subchondral bone changes in SpA.
A 6-year study on the mortality dynamics of sprouts germinated on Schima superba after a severe ice storm in southern China
Authors: Cao Yonghui , Zhou Benzhi , Wang Xiaoming
Published: May 2023

Introduction

Natural disturbances modify forest structure by affecting regeneration dynamics and can change main ecosystem functions. An ice storm unusually took place in southern China in early 2008, which caused huge damage to forests. Resprouting of woody plants in a subtropical forest has received little attention. The role of survival time and mortality has been assessed for newsprouts after an ice storm.

Methods

In this study, damage types, in addition to the annual number and mortality rates of sprouts for all tagged and sampled resprouted Chinese gugertree (Schima superba Gardner & Champ.) individuals more than or equal to 4 cm in basal diameter (BD), were monitored. A total of six plots (20 m × 20 m) wererecorded in a subtropical secondary forest dominated by S. superba in Jianglang Mountain, China. This investigation had been conducted for six consecutive years.

Results

The results showed that the survival rates of the sprouts were dependent on the year they sprouted. The earlier the year they boomed, the lower the mortality. The sprouts produced in 2008 were of the highest vitality and survival rates. Sprouts of the decapitated trees exhibited a better survival rate than those of uprooted or leaning trees. Sprouting position also plays a role in regeneration. Sprouts at the basal trunks of uprooted trees and the sprouts at the upper trunksof the decapitated trees exhibited the lowest mortality. The relationship between the accumulative mortality rate and the average diameter of new sprouts isaffected by damage types.

Discussion

We reported the mortality dynamics of sproutsin a subtropical forest after a rare natural disaster. This information could serve asa reference for the construction of a branch sprout dynamic model ormanagement of forest restoration after ice storms.
The effect of simulated natural environments in virtual reality and 2D video to reduce stress
Authors: Suseno Bayu , Hastjarjo Thomas Dicky
Published: May 2023

Stress is a common problem associated with poor physical and psychological health. Exposure to the natural environment is one method for reducing stress. The real and simulated natural environments have a restorative effect on stress reduction. In contrast to the real environment, simulated natural environments, such as virtual reality and 2D video, provide safer and more controllable exposure. Several studies on the restorative effects of the natural environment in virtual reality and 2D video have been conducted. However, the difference between the two in reducing stress must be clarified. This study was conducted to determine the effect of the simulated natural environments in virtual reality and 2D video and their differences in reducing stress. This study hypothesizes that both simulated natural environments in virtual reality and 2D video can reduce stress, but there is a difference between them in reducing stress. Fifty-three subjects were divided into two experimental groups: 2D video (n = 28) and virtual reality (n = 25). The results indicated that simulated natural environments in virtual reality and 2D video reduced stress. However, there was no difference between the two groups regarding stress reduction.
Sexuality, fertility, family planning, family life, and partnership in young breast cancer patients: a longitudinal study
Authors: Jäkel Karoline , Richter Diana , Leuteritz Katja , Sender Annekathrin , Hinz Andreas
Published: May 2023

Objectives

Adolescent and young adult (AYA) breast cancer patients are often faced with sexuality-related problems. Since healthcare providers are often unfamiliar with problems specific to AYA cancer this topic is too little integrated into routine oncological care. The objective of this study was to analyze sexuality, fertility, family planning, family life, and partnership regarding satisfaction and supportive care needs in AYA breast cancer patients.

Methods

A total of 139 AYA breast cancer patients were examined twice, 1 year apart. The patients were asked to complete several questionnaires and to answer multiple questions about satisfaction with sexuality, fertility, family planning, family life, and corresponding supportive care needs in these domains.

Results

While the patients were largely satisfied with their family life and partnerships, they were less satisfied with their sexuality and family planning. Only small mean score changes were observed in these variables over the course of a year. Being a parent already and having the possibility of further completing family planning were strongly associated with higher satisfaction and lower supportive care needs in these domains. Satisfaction was generally negatively associated with supportive care needs. Older age was predictive of lower satisfaction with sexuality at follow-up.

Conclusion

AYA cancer patients deserve special consultations concerning the impact of cancer and treatment on their sexuality and fertility, and it is especially important that women who have yet to complete their family planning be actively offered information and support concerning sexuality and fertility protection before beginning treatment.
Does believing something to be fiction allow a form of moral licencing or a ‘fictive pass’ in understanding others’ actions?
Authors: Thompson Jacqueline , Teasdale Ben , van Emde Boas Evert , Budelmann Felix , Duncan Sophie , Maguire Laurie , Dunbar Robin
Published: May 2023

Introduction

The human capacity to engage with fictional worlds raises important psychological questions about the mechanisms that make this possible. Of particular interest is whether people respond differently to fictional stories compared to factual ones in terms of how immersed they become and how they view the characters involved and their actions. It has been suggested that fiction provides us with a ‘fictive pass’ that allows us to evaluate in a more balanced, detached way the morality of a character’s behaviour.

Methods

We use a randomised controlled experimental design to test this.

Results and discussion

We show that, although knowing whether a substantial film clip is fact or fiction does not affect how engaged with (‘transported’ by) a troubling story an observer becomes, it does grant them a ‘fictive pass’ to empathise with a moral transgressor. However, a fictive pass does not override the capacity to judge the causes of a character’s moral transgression (at least as indexed by a causal attribution task).
Neuropsychological assessment of Romanian burning mouth syndrome patients: stress, depression, sleep disturbance, and verbal fluency impairments
Authors: Dugan Cosmin , Popescu Bogdan Ovidiu , Țovaru Serban , Părlătescu Ioanina , Musat Ioana Andreea , Dobre Maria , Ribigan Athena Cristina , Milanesi Elena
Published: May 2023

Burning Mouth Syndrome (BMS) is a chronic condition characterized by a burning sensation in the oral mucosa, lasting more than 2 hours daily for more than 3 months, without clinical and/or laboratory evidence. BMS is often comorbid with mood, and psychiatric disorders, and a complex pathophysiology and interaction between impairments in nociceptive processing and psychologic function is occurring. In this work, we aimed to define the neuropsychological profile specific for BMS patients for a better management of this complex disease. We conducted a case–control study comparing 120 BMS patients and 110 non-BMS individuals (CTRL). Sociodemographic data and lifestyle habits, were collected, along with data regarding quality of life (SF-36 scale), stress (PSS), depression and anxiety (MADRS and HADS scales), sleep quality (PSQI scale), and cognitive functions (MoCA, SVF and PVF tests). The statistical analysis revealed a lower general quality of life (p < 0.001), worse sleep quality (p < 0.001) in BMS patients than CTRL. The BMS patients also displayed a higher prevalence of mild depressive symptoms than CTRL applying the MADRS (p < 0.001) and HADS-Depression scales (p = 0.001), whereas no differences in anxiety symptoms were found between the two groups (p = 0.174). Moreover, reduced scores semantic and phonemic verbal fluency tests (p < 0.05) were found, but no change in cognition was observed through MoCA (p = 0.551). Our results highlight that synergy between dentistry and neuropsychiatric assessment is essential for a successful management of BMS.
Investigating the impact of online language exchanges on second language speaking and willingness to communicate of Chinese EFL learners: a mixed methods study
Authors: Zhou Anju
Published: May 2023

Introduction

The current study aims to investigate the impact of online language exchanges on the speaking skills and willingness to communicate (WTC) of Chinese postgraduate students in an advanced English program. The study compares two intact classes: e-tandem, who communicate with foreign English speakers through the Tandem language exchange application, and conventional, who participate in collaborative speaking tasks inside the class. The study also examines the attitudes and perceptions of the EFL learners toward the online language exchanges.

Methods

58 Chinese postgraduate students were recruited from a second-year advanced English program and divided into two intact classes: e-tandem and conventional. The e-tandem group used the Tandem language exchange application to communicate with foreign English speakers online, while the conventional group participated in collaborative speaking tasks inside the class. The data were collected through the IELTS speaking module, WTC scale, and semi-structured interviews. The data were analyzed using descriptive and inferential statistics.

Results

Both groups showed improvement in their speaking skills and WTC. However, the e-tandem group outperformed the conventional group. The findings indicate that online language exchanges have a positive impact on the speaking skills and WTC of EFL learners. The EFL learners also had positive attitudes and perceptions toward the online language exchanges, although some expressed reservations.

Discussion

The study concludes that online language exchanges can be an effective tool for improving the speaking skills and WTC of EFL learners. The study also suggests that collaborative speaking courses in EFL settings should consider incorporating online language exchanges. However, the study also highlights the need to address the concerns and reservations expressed by some EFL learners regarding online language exchanges. Overall, the study has important pedagogical implications for EFL settings, suggesting that online language exchanges can enhance speaking skills and WTC.
Effectiveness of Illness Management and Recovery program on people with severe mental illnesses: a systematic review and meta-analysis
Authors: Goh Yong Shian Shawn , Ow Yong Jenna Qing Yun , Li Amy Ziqiang
Published: May 2023

Background

The Illness Management and Recovery (IMR) program has been established in response to the challenges faced by people with severe mental illnesses (SMIs). The program emphasizes the self-management of mental health conditions and the achievement of personally meaningful goals. However, reviews on its efficacy remain scarce, especially in recent years.

Objective

This review aimed to examine the efficacy of IMR in improving personal-recovery outcomes among people with SMIs.

Methods

A search was conducted on seven databases (CINAHL, Embase, ProQuest, PsycINFO, PubMed, Scopus, and Web of Science) from inception to February 2022, without limits on the dates and types of publications. Studies were included if they had examined the efficacy of IMR in one or more outcomes, investigated at least one group of participants, and been published in English. The participants were adults (at least 16 years of age) with a formal diagnosis of at least one SMI.

Results

Fourteen studies were included in this review, and eight outcomes were examined: personal recovery, global functioning, social functioning, hope, perceived social support, quality of life, substance abuse, and knowledge of mental illness. There is limited evidence on the superiority of IMR to existing treatment plans or other interventions in improving the outcomes of interest among people with SMIs. However, the low attendance rates in many included studies suggest the presence of a threshold of exposure to IMR beyond which its treatment effects could be observed. Suggestions for future IMR implementation are discussed.

Conclusions

The IMR program may serve as an alternative or complementary intervention for people with SMIs, especially with enhanced program exposure and access to resource materials.

Systematic review registration

https://inplasy.com/inplasy-2022-10-0005/.
Sexual stigmas among lesbian, gay, and bisexual individuals with problematic internet use and depression
Authors: Wang Peng-Wei , Chang Yu-Ping , Tsai Ching-Shu , Yen Cheng-Fang
Published: May 2023

Introduction

Sexual stigma causes psychological distress among lesbian, gay, and bisexual (LGB) individuals. LGB individuals are more likely to exhibit both problematic Internet use (PIU) and significant depression than are heterosexual individuals. Whether the severities of sexual stigma varied among LGB individuals with various statuses of comorbid PIU and depression warrants study. The present study aimed to investigate the differences in the experiences of familial sexual stigma (FSS), internalized sexual stigma (ISS), and sexual orientation microaggressions (SOMs) among LGB individuals with various statuses of comorbid PIU and depression.

Methods

In total, 1,000 self-identified LGB young adult individuals participated in the study. The level of PIU was assessed using the Chen Internet Addiction Scale, depression was assessed using the Center for Epidemiological Studies-Depression Scale, the experience of FSS was assessed using the Homosexuality-Related Stigma Scale, the experience of ISS was assessed using the Measure of Internalized Sexual Stigma for Lesbians and Gay Men, and the experience of sexual orientation microaggression was assessed using the Sexual Orientation Microaggression Inventory. The differences in the levels of FSS, ISS, and SOMs among the four groups [i.e., the groups with both PIU and depression (comorbid group), only depression, only PIU, and neither PIU nor depression (neither group)] were investigated using multivariate analysis of covariance.

Results

The results indicated that LGB individuals with comorbid PIU and depression reported higher levels of ISS and SOMs than did those with depression only and PIU only, respectively. Moreover, LGB individuals with PIU or significant depression had higher levels of FSS and SOMs than did those with neither PIU nor depression.

Discussion

The results of this study supported that the experiences of FSS, ISS, and SOMs were significantly associated with various levels of PIU and depression in LGB individuals.
Chinese families' knowledge, attitudes, and practices regarding seizure management for children with epilepsy: a mixed-methods study
Authors: Cui Cui , Li Shuangzi , Chen Wenjin , Zhou Hengyu , Zheng Xianlan
Published: May 2023

Objective

This study aimed to examine Chinese families' knowledge, attitudes, and practices regarding the management of acute seizures (AS) that occur outside the hospital in children with epilepsy (CWE) and factors that influence AS.

Design

A mixed-methods sequential explanatory study was conducted, which was integrated at the design and methods levels. In phase 1, a questionnaire was developed for this study, and a family functioning assessment was administered from Nov 2021 to Apr 2022. Multivariate logistic regression was used to analyze the knowledge, attitudes, and practices (KAP) and factors that influence AS. In phase 2, family caregivers (FCGs) were recruited from Jul to Aug 2022 to participate in a qualitative exploration, using semi-structured interviews and a combination of inductive and deductive methods.

Setting

The setting was five children's specialty hospitals in different regions of China.

Participants

The participants were FCGs of CWE. A total of 645 participants were included in the quantitative phase, and 15 FCGs (eight parents, five grandparents, and two others) were recruited for the qualitative phase.

Results

The FCGs' average total KAP score for AS management was 66.23 ± 15.12, with 45.42% of FCGs having a low level. Univariate and multivariate regression analyses showed that demographic factors, disease characteristics, and family function significantly predicted family management of AS. The three most salient themes and eight sub-themes from phase 2 were explored. The quantitative and qualitative databases were analyzed separately and combined through integration, and a conceptual model was constructed based on the individual and family self-management theory (IFSMT); the model consisted of context, knowledge, self-regulation, and promotion factors.

Conclusion

Chinese families have a positive attitude toward the management of out-of-hospital AS in CWE, but lack practice and related knowledge. AS management for CWE families was associated with the demographic characteristics of FCGs, epilepsy, and family characteristics. The research findings expand the existing application requirements of an Acute Seizure Action Plan and patient safety. Our results also indicate a pressing need for localized development of AS emergency medicine in family medicine, the establishment of auxiliary information systems, the utilization of caregivers' positive psychological resources, and improvements in family function for intergenerational care.
Health outcomes and budget impact projection of anti-PD-(L)1s in cancer care in Portugal
Authors: Costa Luís , Alexandre Teresa , Mansinho André , Sousa Rita , Vieira Cláudia , Hughes Robert , Roediger Alexander , Pereira Sónia Matos , Araújo António
Published: May 2023

Introduction

PD-[L]1 inhibitors revolutionized cancer treatment but challenge the affordability of health systems. This policy-focused model aimed to estimate the health and budget impact of anti-PD-(L)1s in Portugal and inform current discussions.

Materials and methods

The Health Impact Projection (HIP) model estimates clinical (life years, progression-free survival [PFS] years, and quality-adjusted life years [QALY] gained and adverse events [AEs] incurred) and economic (direct and indirect costs) outcomes in a world where cancer patients are initiating treatment with standard-of-care (SOC) versus SOC plus anti-PD-(L)1s over a 3-year time horizon. Indications included adjuvant and metastatic melanoma, non-small cell lung cancer (first and second line), metastatic triple-negative breast cancer, head and neck cancer, urothelial carcinoma, and renal cell carcinoma. Model inputs were based on publicly available literature data and expert opinion.

Results

The model estimated that, over 3 years, 7,773 patients would be treated with anti-PD-(L)1s, realizing a gain of 4,787 life years, 6,901 PFS years, and 4,214 QALYs and avoiding 399 AEs. The introduction of anti-PD-(L)1s had a projected average annual impact of ≈ €108 million and a share of 20% of total cancer medicines expenditure and 0.6% of total healthcare expenditure in 2021. Although higher disease management costs are expected for patients living longer with anti-PD-(L)1s and drug acquisition costs are considerable, that is partially offset by a reduction in end-of-life costs (€611,092/year) and costs associated with patient productivity lost to cancer (€9,128,142/year).

Discussion

This model highlights the significant survival and QoL benefit of anti-PD-(L)1s for cancer patients in Portugal, with a relatively low increased cost in total healthcare expenditure.
Public health and medical preparedness for mass casualties from the deliberate release of synthetic opioids
Authors: Cibulsky Susan M. , Wille Timo , Funk Renée , Sokolowski Danny , Gagnon Christine , Lafontaine Marc , Brevett Carol , Jabbour Rabih , Cox Jessica , Russell David R. , Jett David A. , Thomas Jerry D. , Nelson Lewis S.
Published: May 2023

The large amounts of opioids and the emergence of increasingly potent illicitly manufactured synthetic opioids circulating in the unregulated drug supply in North America and Europe are fueling not only the ongoing public health crisis of overdose deaths but also raise the risk of another type of disaster: deliberate opioid release with the intention to cause mass harm. Synthetic opioids are highly potent, rapidly acting, can cause fatal ventilatory depression, are widely available, and have the potential to be disseminated for mass exposure, for example, if effectively formulated, via inhalation or ingestion. As in many other chemical incidents, the health consequences of a deliberate release of synthetic opioid would manifest quickly, within minutes. Such an incident is unlikely, but the consequences could be grave. Awareness of the risk of this type of incident and preparedness to respond are required to save lives and reduce illness. Coordinated planning across the entire local community emergency response system is also critical. The ability to rapidly recognize the opioid toxidrome, education on personal protective actions, and training in medical management of individuals experiencing an opioid overdose are key components of preparedness for an opioid mass casualty incident.
Surveillance of carbapenem-resistant organisms using next-generation sequencing
Authors: Gali Katelin V. , St. Jacques Rachael M. , Daniels Cheyanne I. D. , O'Rourke Allison , Turner Lauren
Published: May 2023

The genomic data generated from next-generation sequencing (NGS) provides nucleotide-level resolution of bacterial genomes which is critical for disease surveillance and the implementation of prevention strategies to interrupt the spread of antimicrobial resistance (AMR) bacteria. Infection with AMR bacteria, including Gram-negative Carbapenem-Resistant Organisms (CRO), may be acute and recurrent—once they have colonized a patient, they are notoriously difficult to eradicate. Through phylogenetic tools that assess the single nucleotide polymorphisms (SNPs) within a pathogen genome dataset, public health scientists can estimate the genetic identity between isolates. This information is used as an epidemiologic proxy of a putative outbreak. Pathogens with minimal to no differences in SNPs are likely to be the same strain attributable to a common source or transmission between cases. These genomic comparisons enhance public health response by prompting targeted intervention and infection control measures. This methodology overview demonstrates the utility of phenotypic and molecular assays, antimicrobial susceptibility testing (AST), NGS, publicly available genomics databases, and open-source bioinformatics pipelines for a tiered workflow to detect resistance genes and potential clusters of illness. These methods, when used in combination, facilitate a genomic surveillance workflow for detecting potential AMR bacterial outbreaks to inform epidemiologic investigations. Use of this workflow helps to target and focus epidemiologic resources to the cases with the highest likelihood of being related.
Building resilient partnerships: How businesses and nonprofits create the capacity for responsiveness
Authors: Taylor Lauren A. , Aveling Emma-Louise , Roberts Jane , Bhuiya Nazmim , Edmondson Amy , Singer Sara
Published: May 2023

Increasingly, businesses are eager to partner with nonprofit organizations to benefit their communities. In spite of good intentions, differences between nonprofit and business organizations can limit the ability of potential partnerships to respond to a changing economic and public health landscape. Using a retrospective, multiple-case study, we sought to investigate the managerial behaviors that enabled businesses and nonprofits to be themselves together in sustainable partnerships. We recruited four nonprofit-business partnerships in the Boston area to serve as cases for our study. Each was designed to address social determinants of health. We thematically analyzed qualitative data from 113 semi-structured interviews, 9 focus groups and 29.5 h of direct observations to identify organizational capacities that build resilient partnerships. Although it is common to emphasize the similarities between partners, we found that it was the acknowledgement of difference that set partnerships up for success. This acknowledgement introduced substantial uncertainty that made managers uncomfortable. Organizations that built the internal capacity to be responsive to, but not control, one another were able to derive value from their unique assets.
What Can Tapir Poop Teach Us About Healthy Forests?
Authors: Yanez-Montalvo Alfredo , Gaona Osiris , de León-Lorenzana Arit , Águila Bernardo , Falcón Luisa I. , Neri-Barrios Carla Ximena , Pérez-Flores Jonathan
Published: May 2023

The Baird’s tapir is the largest land mammal living in the Mexican Selva Maya, which is the largest tropical forest in America after the Amazon. Tapirs are herbivores that play a key role in maintaining the health of tropical forests by dispersing seeds in their feces (poop). Tapirs are mysterious, silent, and nocturnal characteristics that make them difficult to study. In our research, we analyzed the microbes in tapirs’ feces to understand the health of tapir populations in the Mexican Selva Maya. We found that a large amount of variety in tapirs’ fecal microbes is associated with a healthy tropical forest ecosystem. Maybe the analysis of the fecal microbes of wildlife can be used as a gentle technique to help us understand the health status of animals and the environments in which they live.
Acute effects of mental recovery strategies in simulated air rifle competitions
Authors: Loch Fabian , Ferrauti Alexander , Meyer Tim , Pfeiffer Mark , Kellmann Michael
Published: May 2023

Objectives

The present study aimed to assess the perception and change of mental and physical fatigue and to examine acute effects of mental recovery strategies in air rifle athletes across simulated competition days with two consecutive competition bouts.

Design

We conducted a randomized counterbalanced crossover study.

Method

22 development air rifle athletes (Mage = 17.77 ± 4.0) of a regional squad participate in the study. The Short Recovery and Stress Scale (SRSS), perception of mental fatigue, physical fatigue, concentration and motivation as well as differential Ratings of Perceived Exertion (RPE) were used to assess recovery-stress states and fatigue states. During a recovery break, participants underwent two mental recovery strategies (powernap, systematic breathing) or a control condition. Total shooting scores were recorded for both competition bouts.

Results

Study results revealed a significant increase of post ratings for mental (p < .001) and physical fatigue (p < .001) for both competition bouts. The correlation coefficient between change in mental and physical fatigue for both competitions revealed a shared variance of 7.9% and 18.6%, respectively. No significant group-based acute effects of the use of mental recovery strategies on shooting performance, and psychological and perceptual measures were found. On an individual level, results illustrated statistical relevant improvements of shooting performance after powernapping or systematic breathing.

Conclusion

Mental and physical fatigue increased and accumulated across a simulated air rifle competition and mental fatigue emerged as a separate construct from physical fatigue. The use of strategies to accelerate mental recovery on an individual level (e.g., powernap, systematic breathing) may be a first step to manage a state of mental fatigue, but further studies on mental recovery strategies in an applied setting are needed.
Estimating 3D ground reaction forces in running using three inertial measurement units
Authors: Scheltinga Bouke L. , Kok Joost N. , Buurke Jaap H. , Reenalda Jasper
Published: May 2023

To understand the mechanisms causing running injuries, it is crucial to get insights into biomechanical loading in the runners' environment. Ground reaction forces (GRFs) describe the external forces on the body during running, however, measuring these forces is usually only possible in a gait laboratory. Previous studies show that it is possible to use inertial measurement units (IMUs) to estimate vertical forces, however, forces in anterior-posterior direction play an important role in the push-off. Furthermore, to perform an inverse dynamics approach, for modelling tissue specific loads, 3D GRFs are needed as input. Therefore, the goal of this work was to estimate 3D GRFs using three inertial measurement units. Twelve rear foot strike runners did nine trials at three different velocities (10, 12 and 14 km/h) and three stride frequencies (preferred and preferred ± 10%) on an instrumented treadmill. Then, data from IMUs placed on the pelvis and lower legs were used as input for artificial neural networks (ANNs) to estimate 3D GRFs. Additionally, estimated vertical GRF from a physical model was used as input to create a hybrid machine learning model. Using different splits in validation and training data, different ANNs were fitted and assembled into an ensemble model. Leave-one-subject-out cross-validation was used to validate the models. Performance of the machine learning, hybrid machine learning and a physical model were compared. The estimated vs. measured GRF for the hybrid model had a RMSE normalized over the full range of values of 10.8, 7.8 and 6.8% and a Pearson correlation coefficient of 0.58, 0.91, 0.97 for the mediolateral direction, posterior-anterior and vertical direction respectively. Performance for the three compared models was similar. The ensemble models showed higher model accuracy compared to the ensemble-members. This study is the first to estimate 3D GRF during continuous running from IMUs and shows that it is possible to estimate GRF in posterior-anterior and vertical direction, making it possible to estimate these forces in the outdoor setting. This step towards quantification of biomechanical load in the runners' environment is helpful to gain a better understanding of the development of running injuries.
Liquid fermented cereals with added Pediococcus acidilactici did not reduce post-weaning diarrhea in pigs – an Escherichia coli challenge study
Authors: Xu Jiajia , Noel Samantha Joan , Lauridsen Charlotte , Lærke Helle Nygaard , Canibe Nuria
Published: May 2023

The effect of feeding fermented liquid feed (FLF) with added Pediococcus acidilactici to weaning piglets challenged with enterotoxigenic Escherichia coli (ETEC) F4 on aspects of diarrhea, performance, immune responses, and intestinal epithelial barrier function was investigated. A total of 46 weaners (weaning at 27–30 days of age) were assigned to four treatments: (1) Non-challenged and dry feed (Non-Dry); (2) Challenged and dry feed (Ch-Dry); (3) Non-challenged and FLF (Non-Ferm); (4) Challenged and FLF (Ch-Ferm). All groups received the same feed, either dry (Non-Dry and Ch-Dry), or in liquid form (Non-Ferm and Ch-Ferm) in which the cereals with added P. acidilactici (106 CFU/g cereals) had been fermented for 24 h at 30°C. On day 1 and 2 post weaning, Ch-Dry and Ch-Ferm were orally inoculated with 5 mL × 109 CFU ETEC F4/mL, whereas the Non-Dry and Non-Ferm received the same amount of saline. Fecal samples and blood samples were collected through the study period. The microbial composition, concentration of microbial metabolites and nutrient composition indicated that the quality of the FLF was high. In the first week, ADFI of both non-challenged groups was significantly higher (p < 0.05) than that of the Ch-Ferm group. The two challenged groups had higher fecal levels of FaeG gene (ETEC F4 fimbriae) from day 2 to 6 post weaning (p < 0.01), and higher risk of having ETEC F4 present in feces from day 3 to 5 post weaning (p < 0.05) compared to non-challenged groups, indicating the validity of the ETEC challenge model. Generally, ADG of the two groups fed FLF were numerically higher than those fed dry feed. Neither challenge nor FLF affected diarrhea. No significant differences were measured between Ch-Ferm and Ch-Dry regarding the level of plasma haptoglobin and C-reactive protein, hematological parameters or parameters related to epithelial barrier. The data indicated a low level of infection caused by the ETEC challenge, while recovery from weaning stress could be observed. The study showed that a strategy like this can be a way of providing a high level of probiotics to pigs by allowing their proliferation during fermentation.
Effect of Cyberlindnera jadinii supplementation on growth performance, serum immunity, antioxidant status, and intestinal health in winter fur-growing raccoon dogs (Nyctereutes procyonoides)
Authors: Zhao Dehui , Zhang Haihua , Liu Keyuan , Wu Yan , Zhang Borui , Ma Cuiliu , Liu Hanlu
Published: May 2023

Introduction

This study aimed to investigate the effects of Cyberlindnera jadinii supplementation on the growth performance, nutrient utilization, serum biochemistry, immunity, antioxidant status, and intestinal microbiota of raccoon dogs during the winter fur-growing period.

Methods

Forty-five 135 (±5) day-old male raccoon dogs were randomly assigned to three dietary groups supplemented with 0 (group N), 1 × 109 (group L) and 5 × 109 CFU/g (group H) Cyberlindnera jadinii, with 15 raccoon dogs per group.

Results

The results showed that Cyberlindnera jadinii in groups L and H improved average daily gain (ADG) and decreased feed-to-weight ratio (F/G) (P < 0.05). No significant difference was found in nutrient digestibility and nitrogen metabolism among the three groups (P > 0.05). Compared with group N, serum glucose levels were lower in groups L and H (P < 0.05). The levels of serum immunoglobulins A and G in group L were higher than those in the other two groups (P < 0.05), and the levels of serum immunoglobulins A and M in group H were higher than those in group N (P < 0.05). Supplementation with Cyberlindnera jadinii in groups L and H increased serum superoxide dismutase activity, and the total antioxidant capacity in group H increased compared with group N (P < 0.05). The phyla Bacteroidetes and Firmicutes were dominant in raccoon dogs. The results of principal coordinate analysis (PCoA) showed that the composition of microbiota in the three groups changed significantly (P < 0.05). The relative abundance of Campylobacterota was increased in the H group compared to the N and L groups (P < 0.05). The relative abundance of Sarcina was increased in group L compared with the other two groups (P < 0.05), while the relative abundance of Subdoligranulum and Blautia were decreased in group H compared with the other two groups (P < 0.05). Also, the relative abundance of Prevotella, Sutterella and Catenibacterium was higher in group L (P < 0.05) compared with group H.

Discussion

In conclusion, dietary supplementation with Cyberlindnera jadinii improved growth performance, antioxidant activity, immune status, and improved intestinal microbiota in winter fur-growing raccoon dogs. Among the concentrations tested, 1 × 109 CFU/g was the most effective level of supplementation.
Autologous platelet-rich fibrin promotes wound healing in cats
Authors: Changrani-Rastogi Anamika , Swadi Krutika , Barve Mitali , Bajekal Niyati
Published: May 2023

Street cats commonly present large skin wounds that pose significant challenges in veterinary practice. Platelet-rich fibrin (PRF) is a second-generation platelet concentrate increasingly used in humans to promote wound healing. Ease of use and clinical success in humans has prompted interest in using PRF in veterinary practice. However, until now, there is no reported study on the use of autologous PRF in feline wound management. This study evaluated the effect of application of autologous PRF in cats with naturally occurring cutaneous wounds. 16 cats with full-thickness cutaneous acute/subacute wounds were randomly allocated to PRF or Control (standard care) groups. Each cat was enrolled for 2 weeks. PRF was prepared according to previously described procedures. PRF was applied on Days 1 and 4 in addition to standard wound care. Wound size was measured using tracing planimetry. Wound surface area was calculated using SketchAndCalc™ software on scanned tracing images. Average wound sizes at enrolment were 8.39 cm2 (Control) (standard deviation (SD) 5.08 cm2) and 9.18 cm2 (PRF) (SD 3.71 cm2) (range 2.42–15.97 cm2). By Day 14, the mean wound size for the Control group was 2.17 cm2 (SD 1.52 cm2) and for the PRF was 0.62 cm2 (SD 0.44 cm2) (p = 0.015). At Day 14, the PRF group showed mean 93.85% wound contraction with SD 3.66, while the control group showed mean 76.23% wound contraction with SD 5.30 (p = <0.0001). Based on the results, PRF could be further investigated to promote wound healing in cats as a low-risk and convenient adjunctive therapy.
Diflunisal Attenuates Virulence Factor Gene Regulation and Phenotypes in Staphylococcus aureus
Authors: Chan Liana , Park Mihyun , Lee Hong , Chaili Siyang , Xiong Yan , Bayer Arnold , Proctor Richard , Yeaman Michael
Published: May 2023

Virulence factor expression is integral to pathogenicity of Staphylococcus aureus. We previously demonstrated that aspirin, through its major metabolite, salicylic acid (SAL), modulates S. aureus virulence phenotypes in vitro and in vivo. We compared salicylate metabolites and a structural analogue for their ability to modulate S. aureus virulence factor expression and phenotypes: (i) acetylsalicylic acid (ASA, aspirin); (ii) ASA metabolites, salicylic acid (SAL), gentisic acid (GTA) and salicyluric acid (SUA); or (iii) diflunisal (DIF), a SAL structural analogue. None of these compounds altered the growth rate of any strain tested. ASA and its metabolites SAL, GTA and SUA moderately impaired hemolysis and proteolysis phenotypes in multiple S. aureus strain backgrounds and their respective deletion mutants. Only DIF significantly inhibited these virulence phenotypes in all strains. The kinetic profiles of ASA, SAL or DIF on expression of hla (alpha hemolysin), sspA (V8 protease) and their regulators (sigB, sarA, agr (RNAIII)) were assessed in two prototypic strain backgrounds: SH1000 (methicillin-sensitive S. aureus; MSSA) and LAC-USA300 (methicillin-resistant S. aureus; MRSA). DIF induced sigB expression which is coincident with the significant inhibition of RNAIII expression in both strains and precedes significant reductions in hla and sspA expression. The inhibited expression of these genes within 2 h resulted in the durable suppression of hemolysis and proteolysis phenotypes. These results indicate that DIF modulates the expression of key virulence factors in S. aureus via a coordinated impact on their relevant regulons and target effector genes. This strategy may hold opportunities to develop novel antivirulence strategies to address the ongoing challenge of antibiotic-resistant S. aureus.
Melatonin as a Chronobiotic/Cytoprotective Agent in REM Sleep Behavior Disorder
Authors: Cardinali Daniel P. , Garay Arturo
Published: May 2023

Dream-enactment behavior that emerges during episodes of rapid eye movement (REM) sleep without muscle atonia is a parasomnia known as REM sleep behavior disorder (RBD). RBD constitutes a prodromal marker of α-synucleinopathies and serves as one of the best biomarkers available to predict diseases such as Parkinson disease, multiple system atrophy and dementia with Lewy bodies. Most patients showing RBD will convert to an α-synucleinopathy about 10 years after diagnosis. The diagnostic advantage of RBD relies on the prolonged prodromal time, its predictive power and the absence of disease-related treatments that could act as confounders. Therefore, patients with RBD are candidates for neuroprotection trials that delay or prevent conversion to a pathology with abnormal α-synuclein metabolism. The administration of melatonin in doses exhibiting a chronobiotic/hypnotic effect (less than 10 mg daily) is commonly used as a first line treatment (together with clonazepam) of RBD. At a higher dose, melatonin may also be an effective cytoprotector to halt α-synucleinopathy progression. However, allometric conversion doses derived from animal studies (in the 100 mg/day range) are rarely employed clinically regardless of the demonstrated absence of toxicity of melatonin in phase 1 pharmacological studies with doses up to 100 mg in normal volunteers. This review discusses the application of melatonin in RBD: (a) as a symptomatic treatment in RBD; (b) as a possible disease-modifying treatment in α-synucleinopathies. To what degree melatonin has therapeutic efficacy in the prevention of α-synucleinopathies awaits further investigation, in particular multicenter double-blind trials.
Analysis and Design of Confined Masonry Structures: Review and Future Research Directions
Authors: Borah Bonisha , Kaushik Hemant B. , Singhal Vaibhav
Published: May 2023

This article discusses the significance of confined masonry (CM) structures in terms of their remarkable seismic performance in past earthquake events. However, the variability of CM structures with differing materials, detailing, and construction practices across different regions poses challenges in developing standardized design guidelines. To address the challenges, the state-of-the-art developments in CM are comprehensively reviewed in the present article. This review encompasses experimental campaigns studying CM walls and buildings to evaluate the effect of important parameters on their performance, a discussion of various numerical and analytical models with their respective benefits and limitations, and an examination of design procedures for CM in nine country codes and their local guidelines. This review identifies gaps in the current knowledge, including the need for more studies on the performance of CM structures under earthquake loads and the use of new materials and construction techniques. The article concludes by formulating future research directions to address the identified gaps, including the need for more experimental studies and the development of sophisticated numerical models that can capture the complexities of these structures under the action of different loads. Overall, the article serves as a valuable resource for researchers and practitioners working on the analysis, design, and construction of CM.
Bimaxillary Dentoalveolar Protrusion Case Treated with Anchorage by Buccally Implemented Mini-Implants Using a 3D-Printed Surgical Guide
Authors: Vasoglou Georgios , Patatou Athanasia , Vasoglou Michail
Published: May 2023

The article presents a case of bimaxillary dentoalveolar protrusion treated by distalizing the upper and lower teeth, using anchorage from mini implants. A 16-year-old male patient presented with severe upper and lower incisor proclination with protruding lips and a convex profile, with a background of bimaxillary dentoalveolar protrusion. Instead of having four premolars extracted, retraction of the dentition was decided with absolute anchorage, provided by mini implants. In order to carry out the procedure in one stage, four mini-implants were inserted as close to the root of the 1st molars as possible. Implementation was facilitated by a surgical template which was created on a digital model and then 3D printed. Accurate placement was achieved and the case was successfully treated by significant uprighting of the incisors and retraction of the anterior dentition, closing the spaces in the upper and lower arch. Facial aesthetics were also improved. A digitally designed surgical guide was utilized in this case of bimaxillary dentoalveolar protrusion in order to facilitate the accurate placement of the mini implants which were used for a one-stage retraction of the dentition.
Indoor Fire Simulation in Low-Rise Teaching Buildings Based on BIM–FDS
Authors: Liu Mengkai , Wang Guoquan
Published: May 2023

School buildings gather a large number of underage students, and the disastrous consequences of fire in such buildings are very serious, which is one of the key concerns of society in fire prevention and control. This study takes a “[” type kindergarten teaching building as the background and constructs a BIM–FDS building fire simulation model to reveal the fire smoke dispersion law under the coupling of the typical building structure and fire protection systems. The results show that the stairwells on both sides of the “[” type building are the main channels for the diffusion of fire smoke, and the asymmetry of the stairwell structure will cause apparent differences in the diffusion of smoke. Using the natural smoke exhaust in the stairwells of low-rise buildings does not aggravate the spread of smoke in the building and is conducive to smoke emissions. The high-pressure water mist system is superior to the water spray system in fire extinguishing and controlling room temperature. While it reduces smoke exhaust performance, it does not adversely affect personnel evacuation. This study systematically reveals the law of diffusion of fire smoke from “[”-type teaching buildings, which can support the design of similar building structures, ventilation, fire protection, and the formulation of fire escape plans.
The Mouthparts of Female Blood-Feeding Frog-Biting Midges (Corethrellidae, Diptera)
Authors: Barton Stephan , Virgo Jonas , Krenn Harald W.
Published: May 2023

Females of frog-biting midges (Corethrellidae) obtain their blood meals from male calling frogs. While the morphology of the feeding apparatus is well studied in hematophagous Diptera that impact humans, frog-biting midges have received far less attention. We provide a detailed micromorphological examination of the piercing blood-sucking proboscis and maxillary palpus in three Corethrella species using scanning electron microscopy and histological semi-thin sectioning. We also compare the sensilla found on the proboscis tip and the palpus of Corethrella with other piercing blood-sucking Diptera. Corethrella spp. have a proboscis length of about 135 µm, equipped with delicate mandibular piercing structures composing the food canal together with the labrum and hypopharynx. Their proboscis composition is plesiomorphic and more similar to other short-proboscid hematophagous Culicomorpha (e.g., Simuliidae), in contrast to the phylogenetically more closely related long-proboscid Culicidae. As in other short-proboscid taxa, the salivary canal in Corethrella spp. transitions into an open salivary groove with one mandible forming a seal, whereas in Culicidae the salivary canal is closed until the tip of the proboscis. We discuss the possible functional constraints of very short, piercing blood-sucking proboscises (e.g., dimensions of host blood cells) that may limit the size of the food canal.