bluuebunny/crossref_metadata_2025 huggingface dataset:
Moroccan and Spanish agriculture comparative analysis 2008-2021
Authors: Asllam Lahoucine , Bari Ahmed Ait , Amzil Mustapha
Published: December 2023

In the general context of Morocco's New Development Model, where public policies need all their credentials, and more specifically in the context of Morocco's new agricultural strategy (Green Plan), the challenge is to develop modern agriculture with high added value and high productivity, capable of competing with agricultural production in other countries. This article compares the overall characteristics of agricultural production in Spain and Morocco. To make this comparative analysis, we have focused on a set of agricultural statistical aggregates, such as cultivated area, level of production and yield in relation to each family of agricultural products. These are calculated, using the Laspeyres index, over the period 2008 to 2021. In addition, we have chosen 2008 as the base year for plotting the evolution of Moroccan agricultural performance against that of Spain. The results of our comparative analysis showed that agriculture in Spain is more stable than in Morocco, particularly in cereals and fresh vegetables. Thus, they showed that the majority of Spanish agricultural products outperform those of Morocco. On the other hand, this comparative analysis showed that Moroccan agriculture outperforms Spanish agriculture in the production of sheep, apples, fresh peas, carrots, turnips, broad beans, green beans, goat meat, dried lentils and chickpeas, and that Moroccan agriculture outperforms Spanish agriculture in the production of dates and unshelled peanuts.
The role of information communication technologies on African indigenous knowledge systems: Folktales
Authors: Roya Witness , Ngcobo Sandiso
Published: December 2023

African Indigenous Knowledge Systems (AIKS) are values that were passed among African generations by word of mouth. Despite the Fourth Industrial Revolution's (4IR) facilitation of rapid communication for every aspect of society, many Africans have not embraced it to store AIKS, mainly due to concerns over biases and prejudices embedded in aspects like information and communication technologies (ICTs). Using decoloniality theory, this paper aims to show the importance of AIKS, which faces extinction due to the combined effects of the deaths of members of the old generation and acculturation. In the methodology section, Afrocentricity, which celebrates rootedness in African culture and history, was used together with storytelling as a means to examine the perceptions of Afrocentric scholars as revealed in their folktale works that were analyzed qualitatively. Results revealed that lack of exposure to ICTs is one of the main reasons for the scant AIKS virtual literature. Many African people do not have access to other needs like food, education, clothing, electricity, and shelter, which effectively renders ICTs ‘secondary, yet access to information and participation are fundamental rights. We urge stakeholders to implement policies that promote the preservation of AIKS in electronic repositories. Memory devices and other ICT accessories should be responsive to African cultures. Investment in ICT infrastructure and training, reliable electricity, and addressing all the basics are critical.
PENGEMBANGAN PERANGKAT PEMBELAJARAN MODEL, CREATIVE PROBLEM SOLVING (CPS) UNTUK MENINGKATKAN LITERASI SAINS PADA MATERI SUHU DAN PERUBAHANNYA
Authors: Paramitha Devi Dini , Yunus Rahmat , Abdullah Abdullah
Published: December 2023

Penelitian ini bertujuan untuk mengembangkan bahan pembelajaran dan media yang mendukung proses pembelajaran yang aktif, inovatif, kreatif, efektif, dan menyenangkan serta dapat mengembangkan metode pemecahan masalah secara kreatif (creative problem solving). Perangkat pembelajaran meliputi silabus, RPP, materi ajar, LKPD, dan LP. Penelitian ini merupakan penelitian pengembangan dengan menggunakan model pengembangan Tessmer yang meliputi evaluasi diri, uji ahli, uji perorangan, uji kelompok kecil, dan uji lapangan. Subjek uji coba penelitian adalah 27 peserta didik kelas VIIA dan 25 peserta didik kelas VIIB SMP Negeri 2 Sampanahan. Data hasil penelitian menunjukkan bahwa perangkat pembelajaran ini telah memenuhi nilai validitas, kepraktisan dan keefektifan perangkat pembelajaran. Perangkat Pembelajaran CPS layak untuk meningkatkan literasi sains pada peserta didik karena perangkat pembelajaran yang di kembangkan sudah valid, praktif, serta efektif.
PENGGUNAAN CULTURE LITERACY DIGITAL WETLAND (CLDW) LKPD KONSEP PERUBAHAN LINGKUNGAN TERHADAP PENGUASAAN KONSEP DAN KETERAMPILAN GENERIK SAINS PESERTA DIDIK KELAS X MIPA SMA NEGERI 8 BANJARMASIN
Authors: Wulandari Evi , Putra Aminuddin Prahatama , Rezeki Amalia
Published: December 2023

Penelitian ini bertujuan untuk (1) mendeskripsikan penggunaan Culture Literacy Digital Wetland (CLDW) LKPD berpengaruh secara signifikan terhadap penguasaan konsep peserta didik kelas X MIPA 1 SMAN 8 Banjarmasin pada meteri perubahan lingkungan (2) Mendeskripsikan Penggunaan Culture Literacy Digital Wetland (CLDW) LKPD berpengaruh secara signifikan terhadap keterampilan generik sains konsep peserta didik kelas X MIPA 1 SMAN 8 Banjarmasin pada meteri perubahan lingkungan. Penelitian ini menggunakan pendekatan quasi eksperimen dengan design The Non-equivalent Control Group Design untuk penguasaan konsep dan keterampilan generik sains. Sampel penelitian ini adalah peserta didik  X MIPA 1 diberi perlakuan sebagai kelas eksperimen dan X MIPA 3 sebagai kelas kontrol. Teknik analisis data untuk hasil penguasaan konsep menggunakan uji T non-paramterik yaitu Uji Mann-Whitney  dan untuk hasil keterampilan generik sains menggunakan uji Independent Sampel T-Test .Hasil penelitian ini menunjukan (1) tidak terdapat pengaruh Penggunaan Culture Literacy Digital Wetland (CLDW)  LKPD Konsep Perubahan Lingkungan Terhadap Penguasaan Konsep dan Kereampilan Generik Sains Peserta Didik dengan Asymp-Sig(2-Tailed) 0,0790,05. (2) terdapat pengaruh penggunaan Culture Literacy Digital Wetland (CLDW)  LKPD Konsep Perubahan Lingkungan Terhadap Penguasaan Konsep dan Keterampilan Generik Sains Peserta didik dengan t-hitung 1,99 t-tabel-5,81.Dari hasil penelitian penulis merekomendasikan diperlukannya perbaikan dan pendalaman pemahaman terhadap istilah-istilah asing yang digunakan dalam penelitian dan diperlukannya perbaikan dan diperlukan perbaikan dan penyempurnaan alat evaluasi agar terlihat lebih efektif pengaruhnya terhadap hasil penelitian.
Bile acids, gut microbiota, and therapeutic insights in hepatocellular carcinoma
Authors: Song Yang , Lau Harry CH , Zhang Xiang , Yu Jun
Published: December 2023

Hepatocellular carcinoma (HCC) is a prevalent and aggressive liver malignancy. The interplay between bile acids (BAs) and the gut microbiota has emerged as a critical factor in HCC development and progression. Under normal conditions, BA metabolism is tightly regulated through a bidirectional interplay between gut microorganisms and BAs. The gut microbiota plays a critical role in BA metabolism, and BAs are endogenous signaling molecules that help maintain liver and intestinal homeostasis. Of note, dysbiotic changes in the gut microbiota during pathogenesis and cancer development can disrupt BA homeostasis, thereby leading to liver inflammation and fibrosis, and ultimately contributing to HCC development. Therefore, understanding the intricate interplay between BAs and the gut microbiota is crucial for elucidating the mechanisms underlying hepatocarcinogenesis. In this review, we comprehensively explore the roles and functions of BA metabolism, with a focus on the interactions between BAs and gut microorganisms in HCC. Additionally, therapeutic strategies targeting BA metabolism and the gut microbiota are discussed, including the use of BA agonists/antagonists, probiotic/prebiotic and dietary interventions, fecal microbiota transplantation, and engineered bacteria. In summary, understanding the complex BA-microbiota crosstalk can provide valuable insights into HCC development and facilitate the development of innovative therapeutic approaches for liver malignancy.
A Study of College Students’ Experience in Online Learning with Gender Differences
Authors: Mutawa A.M , Sruthi Sai
Published: December 2023

Educational institutions worldwide have adopted e-learning due to the COVID-19 pandemic. While this shift to online learning (OL) has presented challenges for students and teachers, it has also sparked innovative educational approaches. This study investigates college students' perceptions regarding OL, specifically focusing on gender differences in the experience with OL A quantitative survey gathered information from distinct college students in Gulf Cooperation Council (GCC) nations who experienced OL during the pandemic. The survey included questions related to satisfaction with OL and specific aspects such as course content delivery, interaction with instructors, behavioral changes, and challenges with OL. Male and female student OL experience was compared statistically. The results conclude that male students are more satisfied than female students. Students struggled with internet connection, OL adaptation, focus, and workload during OL. Understanding these gender differences in students' satisfaction with OL is crucial for educators and institutions as they strive to optimize the effectiveness of e-learning strategies. By recognizing and addressing male and female students' unique needs and challenges, educational institutions can enhance the overall educational experience during times of crisis and beyond.
Cx1Mab-1: A Novel Anti-mouse CXCR1 Monoclonal Antibody for Flow Cytometry
Authors: Li Guanjie , Tanaka Tomohiro , Suzuki Hiroyuki , Kaneko Mika K , Kato Yukinari
Published: December 2023

The C-X-C motif chemokine receptor-1 (CXCR1) is a rhodopsin-like G-protein-coupled receptor, expressed on the cell surface of immune cells and tumors. CXCR1 interacts with some C-X-C chemokines, such as CXCL6, CXCL7, and CXCL8/interleukin-8, which are produced by various cells. Since CXCR1 is involved in several diseases including tumors and diabetes mellitus, drugs targeting CXCR1 has been developed. Therefore, the development of sensitive monoclonal antibodies (mAbs) for CXCR1 has been desired for the diagnosis and treatment. This study established a novel anti-mouse CXCR1 (mCXCR1) mAb; Cx1Mab-1 (rat IgG1, kappa), using the Cell-Based Immunization and Screening (CBIS) method. Cx1Mab-1 reacted with mCXCR1-overexpressed Chinese hamster ovary-K1 (CHO/mCXCR1) and mCXCR1-overexpressed LN229 glioblastoma (LN229/mCXCR1) in flow cytometry. Cx1Mab-1 demonstrated a high binding affinity for CHO/mCXCR1 and LN229/mCXCR1 with a dissociation constant of 2.6×10-9 M and 2.1×10-8 M, respectively. Furthermore, Cx1Mab-1 could detect mCXCR1 by western blot analysis. These results indicated that Cx1Mab-1 is useful for detecting mCXCR1, and provides a possibility for targeting mCXCR1-expressing cells in vivo experiments.
Knowledge on Stroke Recognition and Management Among Emergency Department Healthcare Professionals in the Republic of Cyprus
Authors: Rossis Christos , Michail Koralia A. , Middleton Nicos , Karanikola Maria , Papathanassoglou Elizabeth , Mpouzika Meropi
Published: December 2023

Stroke is a global leading cause of death and disability. Knowledge of related guidelines is crucial for Emergency Department (ED) staff, influencing early diagnosis and timely treatment. We investigated Greek-Cypriot ED healthcare professionals' (nurses and physicians) knowledge in recognizing and managing stroke. A descriptive cross-sectional study spanned November 2019 to April 2020, encompassing four private and seven public EDs in the Republic of Cyprus. The data were collected through a self-reported questionnaire developed by the research team, consisting of 37 questions. Eight questions focused on sociodemographic and employment characteristics, 28 assessed knowledge in stroke recognition and management (each item was equally weighted without deliberate prioritization), and one question addressed self-assessment of knowledge in stroke care. 255 nurses [Response Rate (RR): 74.1%] and 26 physicians (RR: 47.3%) completed the questionnaire. The average correct response rate was 12.9 out of 28 statements (SD: 4.2), with nurses and physicians scoring 12.6 (SD: 4.1) and 15.7 (SD: 4), respectively. Work experience significantly influenced stroke knowledge, with all groups demonstrating superiority over those with less than one year of experience. Participants with previous training scored an average of 1.45 additional correct answers while educational attainment did not significantly influence stroke knowledge. Investigating stroke knowledge among Emergency Department nurses and physicians in the Republic of Cyprus revealed significant deficits. The study stresses targeted interventions, including, education, yearly examinations, workshops with hands-on training, and repeated training, to address these gaps and enhance the overall stroke care capabilities of the healthcare professionals.
Stat of the Art in Wearable Wrist Exoskeletons Part II: A Review of Commercial and Research Devices
Authors: Pitzalis Roberto Francesco , Park Daegeun , Caldwell Darwin G. , Berselli Giovanni , Ortiz Jesús
Published: December 2023

Manual handling tasks, both in daily activities and at work, require high dexterity and the ability to move objects of different shapes and sizes. However, musculoskeletal disorders that can arise due to aging, disabilities, overloading, or strenuous work can impact the natural capabilities of the hand with serious repercussions both in working and daily activities. To address this, researchers have been developing and proving the benefits of wrist exoskeleton., This paper, which is the part II of a study on wrist exoskeletons, presents and summarizes wearable wrist exoskeleton devices conceived for use in rehabilitation, assistance, and occupational fields. Exoskeletons considered within the study are those available either in a prototyping phase or on the market. They can support the human wrist by relieving pain or mitigating fatigue while allowing at least one movement. According to the requirements to be met, the majority have been designed active (80%) for higher force/torque transmission, and soft for better kinematic compliance, ergonomics, and safety (13 devices out of 24, more than 50%). Electric motors (11 devices out of 24, almost 50%) and cable transmission (9 devices out of 24, almost 40%) are the most common due to their simplicity, controllability, safety, power-to-weight ratio, and the possibility of remote actuation. As sensing technologies, position and force sensors are widely used in all devices (almost 90%). The control strategy depends mainly on the application domain: for rehabilitation, CPM (Control Passive Motion) is preferred (35% of the devices), while for assistance and occupational purposes AAN (Assistance-As-Needed) is more suitable (38% of the devices). What emerges from this analysis is that while rehabilitation and training are fields in which exoskeletons have been grown more easily and gained some user acceptance (almost 18 devices of which 4 are available on the market), relatively few devices have been designed for occupational aims (6 of which only 2 are available on the market) due to difficulties in meeting the acceptance and needs of users. In this perspective, as a result of the state-of-the-art analysis, the authors propose a conceptual idea of a portable soft wrist exoskeleton for occupational assistance.
Elliptical Space as the Possible Origin of Matter
Authors: Yershov Vladimir N.
Published: December 2023

I argue that the main feature of elliptical space – the topological identification of its antipodal points – might be behind some observational facts on both cosmological and microscopic scales. I use calculated distance moduli of type-Ia supernovae, which are commonly regarded as “standard candles” for distance measure in cosmology. These are compared with the observational data collected in a catalogue called “Pantheon+”. On the microscopic scale I show that a physical elliptical space, in which the mathematical connectedness between antipodal points is replaced with physical connections via the Einstein-Rosen bridges (also called “wormholes”), gives rise to sub-particle structures similar to J.A. Wheeler’s geons. The interactions between these structures leading to their equilibrium configurations can explain the experimentally observed pattern of properties of elementary particles forming the standard model of particle physics. The answer to the question why we have this particular set of fundamental particles might lie in the fundamental properties of elliptical space.
A new Proposal for the Interpretation of the Diagonal Compression Test on Masonry Wallettes: the Identification of Young’s Modulus, Poisson’s Ratio, and Modulus of Rigidity
Authors: Ferretti Elena
Published: December 2023

This paper is the continuation of a previous study, which highlighted some inconsistencies in the RILEM guidelines for the interpretation of the diagonal compression test. Although improved compared to the ASTM guidelines, in fact, the RILEM guidelines underestimate the state of hydrostatic stress induced by the diagonal compression test at the center of the wallette. The new interpretation of the diagonal compression test proposed in this article shows that the RILEM guidelines actually underestimate both the hydrostatic and the deviatoric stress states at the center of the wallette. The new formulation complies with the linear elastic theory and allows us to use the the diagonal compression test to identify the three elastic coefficients of masonry. In particular, it allows the identification of the Poisson ratio, which instead takes on a conventional value in the RILEM and ASTM guidelines. The difference of one order of magnitude between the conventional and proposed Poisson’s ratio is in agreement with the experimental results on another brittle material, namely concrete. Finally, the new proposal fills the gap between the results provided by the two tests usually performed to identify the shear behavior of masonry: the diagonal compression test and the shear-compression test.
A New Dynamic Game-Based Pricing Model for Cloud Environment
Authors: Saadatfar Hamid , Gholampour Ahangar Hamid , Hassannataj Joloudari Javad
Published: December 2023

Resource pricing in cloud computing has become one of the main challenges for cloud providers. The challenge is determining a fair and appropriate price to satisfy users and resource providers. To determine a fair price, it is necessary to consider the conditions and needs of both parties: provider and user. This research tries to provide a pricing mechanism for cloud computing based on game theory. The proposed mechanism takes into account three factors including fault possibility, interaction between virtual machines, and energy consumption to reach a fair price. In the proposed game, the provider decides on the price of the virtual machine which can be offered to the user on each physical machine and the user decides between the virtual machines offered to run its application. The whole game is implemented as a function of the resource broker component. The proposed mechanism is simulated and evaluated using the CloudSim simulator. Its performance is compared with several previous recent mechanisms. According to the results, the proposed mechanism has been able to determine a more reasonable price for the user and the provider which increases the overall profitability of the cloud system.
A 48-Channel High-Resolution Ultrasound Beamforming System for Ultrasound Endoscopy Applications
Authors: Yun Soohyun , Lee Seungah , Bae Joonsung
Published: December 2023

We are introducing a highly efficient 48-channel ultrasound beamforming system ideal for ultrasound endoscopy applications. The system includes a transmitter and a receiver, which allow for low-area, high-resolution imaging acquisition. The transmitter uses a charge redistribution HV (high voltage) scheme to generate three-level pulses that actuate the transducer, implemented with the standard CMOS process for optimal cost and power savings. Meanwhile, the receiver features a sub-array structure and a delay generator that reduces area usage. To achieve high-resolution ultrasound imaging acquisition with low computational power, we developed the Shift Coherence Factor (SCF) algorithm, which is hardware-friendly. This approach delivers a lateral resolution over 20% better than the conventional delay and sum (DAS) algorithm, with a contrast ratio of over 30 dB. The system was implemented in a 180 nm standard CMOS process with an area of 24.98 mm2, power consumption of 8.23 mW per channel, achieving a delay resolution of 8.33 ns, and a low-area implementation of 0.52 mm2 per channel. The system offers high-quality imaging acquisition with minimal additional area and power consumption, which has great potential for 3D imaging or catheterized ultrasound systems.
Exposure and Risk Assessment in Environmental and Occupational Health
Authors: Rashid Md Redowan
Published: December 2023

This analysis examines the wide range of occupational and environmental health risks that affect people in diverse settings. This article emphasizes the importance of conducting exposure and risk assessments to safeguard public health by analyzing various hazards, including arsenic, oil accidents, lead, welding fumes, and mycotoxins. Various methodologies are utilized in these research articles to conduct the study, emphasizing hazard identification, exposure assessment, and risk characterization. The results uncover harmful substances, vulnerable demographics, and occupational duties linked to increased hazards. The report suggests various mitigation strategies, such as targeted interventions, enhanced community efforts, and improved exposure estimates. Notwithstanding the constraints identified in certain studies, the review emphasizes the necessity for continuous investigation to improve assessments of exposure, comprehend the cumulative effects, and examine the wider societal consequences. The significance of sector-specific risk assessments, protective measures, and ongoing monitoring is underscored to ensure mental and physical health protection.
Pharmacophore-Based Approach for the Identification of Potent Inhibitors Against LpxC Enzyme From Salmonella typhi
Authors: Ahmed Mohammad Z. , Alqahtani Ali S. , Shukla Prakash , Pal Sudhir Kumar
Published: December 2023

Salmonella causes various type of disease worldwide with a remarkable pace. Salmonella enteric serotype typhi (S. typhi), a gram-negative bacterium (only cause disease in man) is the mainly causative agent of Typhoid fever. Typhoid fever is most common in poor and unprivileged developing countries of Asia and Africa. One of the major components of virulence factors produced during salmonella infection is Lipid A, which acts as a potent human immuno-modulator bacterial endotoxin. Regulation of Lipid A biosynthetic pathway occurs at second step, catalyzed by LpxC, a Zn2+ dependent metalloamidase. Systematic Screening of a pool of drug datasets like natural products library from Zinc database, Asinex database, Thiophene analogues fruitfully provided us 3 potent lead molecules s1_dl_mseq2, s1_ll_mseq2, and s2_ll_mseq8 which actively binds with LpxC enzyme and could be developed into sound inhibitors of LpxC enzyme after the application of drug development and processing strategies. Wet lab experimentation is required to validate these results for further use.
The Role Of Chest Radiology in Traumatic Injuries: A Comprehensive Narrative Review
Authors: Desai Dev , Malafi Maria Eleni
Published: December 2023

Chest trauma is a critical medical condition that encompasses a range of injuries and can result from various causes. Prompt and accurate diagnosis is crucial in managing chest trauma patients and radiology plays a pivotal role in this aspect. Imaging modalities in chest trauma aid in precise diagnosis and treatment planning. Especially, X-rays remain the initial imaging modality of choice due to their accessibility, speed, and ability to detect acute life-threatening conditions. Computed Tomography (CT) Scan offers detailed cross-sectional images allowing for comprehensive evaluation of chest trauma and surgical planning when necessary. Ultrasound (US) is increasingly valuable tool due to its portability and real-time imaging capabilities, aiding in rapid assessment at the bedside. Magnetic Resonance Imaging (MRI) is less commonly used due to time constraints and limited availability. Radiological imaging serves various purposes in the diagnosis and management of chest trauma such as early detection and triage, characterization and severity assessment, surgical planning, follow-up and monitoring of chest trauma. Reduction of radiation exposure, advancements in imaging technology (dual-energy CT, contrast-enhanced imaging) and Artificial Intelligence (AI) algorithms are crucial challenges and deserve attention. A multidisciplinary approach integrating radiological expertise remains fundamental in ensuring optimal outcomes for patients with chest trauma.
Peri-Carotid Adipose Tissue and Atherosclerosis at Carotid Bifurcation
Authors: Ferreira Joana , Longatto-Filho Adhemar , Dionísio Ana , Correia-Neves Margarida , Cunha Pedro , Mansilha Armando
Published: December 2023

Vulnerable carotid plaques are responsible for 20% of the ischemic strokes. The identification of those asymptomatic carotid plaques that will become symptomatic is essential but remains unclear. Our main goal was to investigate whether the amount of the peri-carotid adipose tissue, estimated by the extra-media thickness (EMT) is associated with the atherosclerotic characteristics at the carotid bifurcation in patients with PAD. An observational, prospective, single-center, longitudinal study was conducted. 177 patients performed carotid Doppler-ultrasound. The following data was collected: EMT, intima-media thickness (IMT), presence of carotid plaques, area of the highest plaque, and grade of internal carotid stenosis. From each carotid bifurcation a right and left EMT was determined. We analyzed both the mean EMT (calculated as the mean between the right and the left EMT) and the EMT ipsilateral to the carotid bifurcation. The presence of carotid plaques was associated with a higher mean EMT, [Median=1.14; IQR=0.66 versus Median=0.97; IQR=0.40; p=.001]. A positive correlation was found between mean EMT and IMT (right: ρ=.20; p=.010; left: ρ=.21; p=.007) and between mean EMT and area of the largest carotid plaque (right: ρ=.17; p=.036; left: ρ=.22; p=.004). Left carotid stenosis ≥ 70% was associated with higher mean EMT. [Median=1.55; IQR=0.68 versus Median=1.00; IQR=0.46; p=.019]. Patients with “acute culprit” carotid stenosis had a higher ipsilateral EMT [Left ipsilateral EMT: Median=1.46; IQR=0.63; “non-acute”: Median=0.94; IQR=0.43; p=.009; Right ipsilateral EMT: Median=2.25; IQR=0.62; “non-acute”: Median=1.00; IQR=0.51; p=.015]. This difference was not found in the contra-lateral EMT. Six months after the neurologic event, EMT ipsilateral to an “acute culprit” carotid stenosis decreased (p=.036). The amount of peri-carotid adipose tissue, estimated with EMT was associated with atherosclerosis at the carotid arteries. In particularly EMT could be a non-invasive marker of “acute culprit” carotid stenosis, easily determined in clinical practice. These findings may have implications in personalized medicine, for the determination of the best treatment approach.
Topic: Land Use and Land Cover in Kotri Jamshoro City, Pakistan, Using Gis and Remote Sensing
Authors: Chandio Mr. Kamran Ali , Saeed Dr. Nagham
Published: December 2023

Due to urban sprawls, the arable land is shifting in the settlement areas and towns, while industrial development is making it more vulnerable. The current study was conducted to see the changes in land use and land cover in Kotri area, Pakistan from 1998 to 2020 to establish trends in recent land use development based on available land use data. In this regard, satellite images in the study area were acquired from USGS and processed using ArcMap 10.5 and Environmental for Visualizing Images (ENVI). Results show that; there was a change in the settlement pattern of Kotri city with 40% between 1998 and 2020, 77% decrease in Bare-land was observed in 2020. Natural vegetation in the study area has decreased significantly with 4.4 Sq/ km in 2020, surface water-bodies in 1998 were 1.3 sq/km and in 2020 there is 1.8 Sq/km indicating 32% increase in surface water bodies. It was further revealed that settlement in Kotri has increased due to its geographical location, nearness of the major city of Hyderabad, and the establishment of the industrial zone and educational city near Kotri. From the foregoing, the study concluded that Kotri City will have more changes in its land use and land cover as the expansion in the city is going on and on. It is therefore recommended that local government and provincial government should pay attention towards this development and put up an action plan towards proper provision for such expansion.
Harmaline and Human ClpP Activation for Pediatric Diffuse Intrinsic Pontine Glioma (DIPG) Treatment
Authors: Miciaccia Morena , Rizzo Francesca , Centonze Antonella , Cavallaro Gianfranco , Contino Marialessandra , Armenise Domenico , Baldelli Olga Maria , Solidoro Roberta , Ferorelli Savina , Scarcia Pasquale , Agrimi Gennaro , Zingales Veronica , Cimetta Elisa , Ronsisvalle Simone , Sipala Federica Maria , Polosa Paola Loguercio , Fortuna Cosimo Gianluca , Perrone Maria Grazia , Scilimati Antonio
Published: December 2023

Diffuse intrinsic pontine glioma (DIPG), affecting children aged 4-7 years, is a rare, aggressive tumor that originates in the pons and then spreads to nearby tissue. DIPG is the leading cause of death for pediatric brain tumors due to its infiltrative nature and inoperability. Radiotherapy has only a palliative effect in stabilizing symptoms. In silico and preclinical studies identified ONC201, as a cytotoxic agent against some human cancer cell lines, including DIPG ones. Single crystal X-ray analysis of the complex of the human mitochondrial caseinolytic serine protease type C (hClpP) and ONC201 (PDB ID: 6DL7) allowed to identify hClpP as its main target. Hyperactivation of hClpP causes damage to mitochondrial oxidative phosphorylation and cell death. In some DIPG patients, receiving ONC201, an acquired resistance was observed. In this context, a wide program was initiated to discover original scaffolds for new hClpP activators to treat ONC201-non-responding patients. Harmaline, a small molecule belonging to the chemical class of β -carboline, was identified through Fingerprints for Ligands and Proteins (FLAP), a structure-based Virtual Screening approach. Molecular dynamics simulations and a deep in vitro investigation showed interesting information on the interaction and activation of hClpP by harmaline.
Fabrication and Optimization of Electrospun Shellac Fibers Loaded with Senna alata Leaf Extract
Authors: Aung Wah Wah , Krongrawa Wantanwa , Limmatvapirat Sontaya , Kulpicheswanich Pattranit , Okonogi Siriporn , Limmatvapirat Chutima
Published: December 2023

Single-fluid electrospinning creates nanofibers from molten polymer solutions with active ingredients. The study utilized a combination of a fractional factorial design and a Box-Behnken design to examine crucial factors among a multitude of parameters and to optimize the electrospinning conditions that impact the fiber mats' morphology and entrapment efficiency of the Senna alata leaf extract. The findings indicated that the shellac content had the greatest impact on both fiber diameter and bead formation. The optimum electrospinning conditions were identified as a voltage of 24 kV, a solution feed rate of 0.8 mL/h, and a shellac-extract ratio of 38.5:3.8. These conditions produced nanosized fibers with a diameter of 306 nm, a low bead-to-fiber ratio of 0.29, and an extract entrapment efficiency of 96% within the fibers. The biphasic profile of optimized nanofibers was confirmed through an in vitro release study. This profile consisted of an initial burst release of 88% within the first hour, which was succeeded by a sustained release pattern surpassing 90% for the next 12 h, as predicted by zero-order release kinetics. The optimized nanofibers demonstrated antimicrobial efficacy against diverse pathogens, suggesting promising applications in wound dressings and protective textiles.
Spectral Analysis of the Metamaterial Formed by Optimally Shaped Omega Elements on a Silicon Substrate at Oblique Incidence of S- and P-polarized Terahertz Radiation
Authors: Lyakhnovich Andrew V , Semchenko Igor V , Samofalov Andrey L , Podalov Maksim A , Sinitsyn George V , Kravchenko Alexandr Y , Khakhomov Sergei
Published: December 2023

The reflection and transmission spectra of a metamaterial formed by omega-shaped elements with pre-calculated optimal parameters on a silicon substrate have been recorded in the terahertz range at oblique incidence for the s- and p- polarizations of the incident wave. The spectra were interpreted within the dipole radiation theory of electromagnetic waves. Both measurement results and analysis provide evidence sup-porting the presence of a pronounced polarization anisotropy impact in the reflection and transmission of the metamaterial. The potential of these materials to be utilized in the development of devices that control the polarization properties of THz radiation across a wide spectral range is examined.
Goiter Prevalence among Druze population in Northern Israel
Authors: Sharabi-Nov Adi , Pundyk Babitzki Ekaterina , Farhat Raed , Edelstein Michael , Avraham Yaniv , Merchavy Shlomo
Published: December 2023

Introduction: Goiter is one of the most common thyroid disorders, yet its etiology is still not completely understood. Previous studies suggested a possible genetic predisposition in combination of environmental factors. The importance of an ethnic hereditary factor in goiter prevalence has rarely been studied. Israel has a diverse ethnic composition: Jews, Muslim and Druze. A previous study of the Israeli Druze has demonstrated that they are genetically a population isolate. The prevalence of goiter in the Israeli Druze has yet to be studied. Objectives: The purpose of this study is to examine goiter prevalence in the Druze population of northern Israel compared with Jewish and Muslim populations in the same region. Methods: A retrospective study of adult patients presented with goiter to the head and neck clinic in Ziv medical center between 2019-2021. Only patients from the same geographical area were included in the analysis. Patient files were reviewed for their demographic data and other goiter related clinical characteristics. Results: A total of 392 patients were included in the study. Of the reviewed files 235 were Jewish, 123 Druze and 34 Muslims. The overall incidence of goiter in the Druze group was 6.92% (CI 5.75-8.25) compared to 4.11% in the Jewish (CI 3.60-4.67) group and 7.21% (CI 4.99-10.08) in the Muslim group. Conclusions: Goiter prevalence in the Druze population is higher compared to the Jewish population in northern Israel. Further studies of possible genetic factors are warranted. A larger study of goiter in the Israeli Muslims is also warranted.
Cultural Itineraries Generated by Smart Data on the Web
Authors: Mazzei Mauro , Capodiferro Cosmo , De Maria Massimo , Spreafico Matteo , V. Bik Oleg , Palma Armando , V. Solovyeva Anna
Published: December 2023

The development of storage standards for databases of different nature and origin makes it possible to aggregate and interact with different data sources in order to obtain and show complex and thematic information to the end user. This article aims to analyze some possibilities opened up by new applications and hypothesize their possible developments. With this work, using the currently available Web technologies, we would like to verify the potential of the use of Linked Open Data in the world of WebGIS and illustrate an application that allows the user to interact with Linked Open Data through their representation on a map. Italy has a artistic and cultural heritage unique in the world and the italian Ministry of Cultural Heritage and Activities and Tourism has created and made freely available a dataset in Linked Open Data format that represents it. With the aim of enhancing and making this heritage more usable, the National Research Council (CNR) has created an application that presents this heritage via WebGIS on a map. Following criteria definable by the user, such as the duration, the subject of interest and the style of the trip, tourist itineraries are created through the places that host this heritage. New possibilities open up where the tools made available by the Web can be used together, according to pre-established sequences, to create completely new applications. This can be compared to the use of words, all known in themselves, which, according to pre-established sequences, allow us to create ever new texts.
Metabolomic Signatures of Brainstem in Mice following Acute and Chronic Hydrogen Sulfide Exposure
Authors: Kim Dong-Suk , Maldonado Cristina MS , Giulivi Cecilia , Rumbeiha Wilson K
Published: December 2023

Hydrogen sulfide (H2S) is an environmental toxicant of health concern. The brain is a major target in acute H2S poisoning. This study was to test the hypothesis that acute and subchronic ambient H2S exposures alter the brain metabolome. Male 7-8 week-old C57BL/6J mice were exposed by whole-body inhalation to 1000 ppm H2S for 45 min and euthanized at 5 min or 72 h for acute exposure. For subchronic study, mice were exposed to 5 ppm H2S 2 h/day, 5 days/week for 5 weeks. The brainstem was removed for metabolomic analysis. Enrichment analysis showed the metabolomic profiles in acute and subchronic H2S exposures matched with those of cerebral spinal fluid from patients with seizures or Alzheimer’s disease. Acute H2S exposure decreased excitatory neurotransmitters, aspartate and glutamate, while the inhibitory neurotransmitter, serotonin, was increased, which may explain H2S-induced loss of consciousness. Branched-chain amino acids and glucose were increased by acute H2S exposure. Subchronic H2S exposure within OSHA guideline surprisingly decreased serotonin altering central nervous system. In subchronic H2S exposure, glucose was decreased while polyunsaturated fatty acids, inosine, and hypoxanthine were increased. Collectively, these results provide important mechanistic clues of acute and subchronic ambient H2S poisonings and show that H2S alters brainstem metabolome.
Circuit training improves the levels of Aβ and BDNF related to cognitive impairment risk factors in obese elderly Korean women
Authors: Kang Duwang , Koh Suhan , Kim Taekyu , Bressel Eadric , Kim Do-yeon
Published: December 2023

The purpose of this study was to investigate the effect of circuit training on β-amyloid, BDNF, and cognitive function in untrained obese elderly Korean women. The subjects for the study were aged 65–70 years, composed of the circuit training group(n=12) and control group(n=11). The 60-minute combined exercise was performed 3 times per week for 16 weeks. Exercise intensity was progressively increased from 40% HRR to 70% HRR. The test data were analyzed by paired t-test, independent t-test, and two-way repeated measures ANOVA, and the alpha level of .05 was set for all tests of significance. The findings of this study were as follows: β-amyloid significantly decreased after the exercise group had compared to before the exercise and BDNF and cognitive function significantly increased after the exercise group had compared to before the exercise. However, there were no significant changes in β-amyloid, BDNF, and cognitive function in the control group Therefore, it is suggested that the circuit training used in this study could be an effective exercise method for improving the risk factors of cognitive impairment in obese elderly Korean women.
GlcNAc6ST2/Chst4 Is Essential for the Synthesis of R-10G-Reactive Keratan Sulfate/Sulfated N-acetyllactosamine Oligosaccharides in Mouse Pleural Mesothelium
Authors: Takeda-Uchimura Yoshiko , Ikezaki Midori , Akama Tomoya O. , Ihara Yoshito , Allain Fabrice , Nishitsuji Kazuchika , Uchimura Kenji
Published: December 2023

We have recently shown that 6-sulfo sialyl N-acetyllactosamine (LacNAc) in O-linked glycan recognized by the CL40 antibody is abundant in the pleural mesothelium under physiological conditions, and that these glycans are complementary synthesized by GlcNAc6ST2 (encoded by Chst4) and GlcNAc6ST3 (encoded by Chst5) in mice. GlcNAc6ST3 is essential for the synthesis of R-10G-positive keratan sulfate (KS) in the brain. The predicted minimum epitope of the R-10G antibody is a dimeric asialo 6-sulfo LacNAc. Whether the R-10G-reactive KS/sulfated LacNAc oligosaccharides are also present in the pleural mesothelium was unknown. If any, the question of which GlcNAc6ST is the responsible enzyme was another issue to be examined as well. Here, we showed that R-10G-reactive glycans are as abundant in the pulmonary pleura as are CL40-reactive glycans, and that GlcNAc6ST3 is only partially involved in the synthesis of these pleural R-10G glycans, unlike in the adult brain. Unexpectedly, GlcNAc6ST2 is essential for the synthesis of R-10G-positive KS/sulfated LacNAc oligosaccharides in the lung pleura. The type of GlcNAc6ST involved in KS glycan biosynthesis and the magnitude of its contribution to the KS synthesis were found to differ among tissues in vivo. We show that GlcNAc6ST2 is required and sufficient for R-10G-reactive KS synthesis in the lung pleura. Interestingly, R-10G immunoreactivity in KSGal6ST (encoded by Chst1) and C6st1 (encoded by Chst3) double-deficient mouse lungs was markedly increased. Muc16, a mucin molecule, was shown to be a candidate carrier protein for the pleural R-10G-reactive glycans. These results suggested that R-10G-positive KS/sulfated LacNAc oligosaccharides may play a role in mesothelial cell proliferation and differentiation. Further elucidation of the functions of sulfated glycans synthesized by GlcNAc6ST2 and GlcNAc6ST3, such as R-10G and CL40 glycans, in pathological conditions may lead to a better understanding of the underlying mechanisms of the physiopathology of lung mesothelium.
Detection of miR-155 Using PNA at Physiological-Like Conditions by Surface Plasmon Resonance and bioFET
Authors: Lavecchia di Tocco Francesco , Botti Valentina , Cannistraro Salvatore , Bizzarri Anna Rita
Published: December 2023

MicroRNAs are small ribonucleotides which act as key gene regulators. Their altered expression is often associated with onset and progression of several human diseases, including cancer. Given their potential use as biomarkers, there is a need to find detection methods of microRNAs suitable for use in clinical setting. Field Effect Transistor based biosensors (bioFETs) appear to be valid tools to detect microRNAs, since they may reliably quantitate the specific binding between the immobilized probe and free target in solution through an easily detectable electrical signal. We have investigated the detection of human microRNA 155 (miR-155) using an innovative cap-turing probe constituted by a synthetic peptide nucleic acid (PNA), which has the advantage to form a duplex even at ionic strengths approaching the physiological conditions. With the aim to develop an optimized BioFET setup, the interaction kinetics between miR-155 and the chosen PNA was preliminary investigated by Surface Plasmon Resonance (SPR). By exploiting both these results and our custom-made bioFET system, we were able to attain a low-cost, real-time, label-free, highly specific detection of miR-155 in the nano-molar range.
Multifaceted Applications of Silver Nanoparticles Synthesized from Marine Fungus Aspergillus flavus MK4: Antimicrobial, Anticancer, and Wound-Healing Properties
Authors: Al-Agamy Mohamed H , Kelany Mahmoud S , Mahdi Wael A , Alshehri Sultan , Alhuzani Mohammad R , Hamed Moaz M
Published: December 2023

Due to their wide range of uses in modern technology, nanomaterial’s of silver metallic (Ag-NPs) have engaged considerable concern, driving significant research with development efforts. This study focuses on investigating the antimicrobial properties of Ag-NPs, encompassing their production, characterization, and biological aspects. The eco-friendly extracellular biosynthetic method employed in this work utilized extracts from the marine fungus Aspergillus flavus MK4 as reducing agents for nanoparticle synthesis. Analyzing the colloidal Ag-NPs using UV-visible spectroscopy revealed a peak in absorbance at 460 nm, indicating the presence of plasmonic properties. The electrons imaging of internal structure (TEM) elucidated spherical shape of the Ag-NPs by 15 nm size. Testing against bacterial diversities (Gr-ve & Gr+ve) revealed potent antibacterial and antifungal efficacy. Cytotoxicity versus HepG-2 cell line was assessed using the MTT assay, and antioxidant properties were examined through the radical scavenging (DPPH) assay. In wise of their conceivable applications in the fields of antibacterial, anticancer, and wound-healing, the Ag-NPs that were synthesized exhibit promising features. With its powerful ability to synthesize silver nanoparticles (Ag-NP), Aspergillus flavus MK4 Aspergillus flavus MK4 can generate nanoparticles extensively characterized, demonstrating their antibacterial, antioxidant, anticancer, and wound-healing properties.
DEEP LEARNING BASED QUESTION ANSWERING SYSTEM (SURVEY)
Authors: Saeed Nayyab , ashraf humaira , Jhanjhi NZ
Published: December 2023

Deep learning-based question answering systems have transformed the discipline of natural language processing (NLP) by automating the extraction of answers from textual data. This survey paper provides a captivating overview of these systems, exploring methodologies, techniques, and architectures such as recurrent neural networks (RNNs), BERT model, and transformer models. Extractive and generative approaches are examined, alongside the challenges of handling complex questions, managing noisy input, and addressing rare or unseen words. This survey serves as a stimulating reference, offering valuable insights to researchers and practitioners, fueling innovation and advancement in question answering systems within NLP.
Mortality Time-Trends of Different Cardiovascular Diseases in a Practically Extinct Cohort of Italian Middle-Aged Men Followed-Up for 61 Years: A Possible Etiological Explanation?
Authors: Puddu Paolo Emilio , Piras Paolo , Menotti Alessandro
Published: December 2023

Aims. In a male Italian cohort (initially aged 40-59, n=1712) followed-up during 61 years and reaching practical extinction, the natural history of major CVD mortality categories including coronary heart disease (CHD), stroke and other Heart Diseases of Uncertain Etiology (HDUE, including congestive heart failure) were studied along with their risk factor relationships.
Methods and Results. Cox models were run with 12 covariates measured at entry as possible predictors. About 93% of all CVD deaths were covered by the 3 major groups selected here (N=751): 37.4% of them were diagnosed as CHD, 30.6% as stroke and 28.5 % as HDUE. CHD declined in the last 20 years of follow-up, while a sharp increase of HDUE mortality was seen. Baseline mean levels of serum cholesterol were 209.6, 204.2 and 198.0 mg/dl respectively for CHD, stroke and HDUE deaths: the multivariable coefficients of serum cholesterol were positive and significant for CHD (p<0.0001) and stroke (p=0.0203) and not significant for HDUE (p=0.3467). In Fine-Gray models, the algebraic signs of cholesterol coefficients were opposite for CHD versus the other mortality categories (t=3.13). The predictive performances of remaining risk factors were varied whereas that of Cox models was not very good, probably due to the attrition phenomenon and possible competing risks.
Conclusion. Large differences in natural history and risk factors were found comparing the 3 CVD conditions, potentially indicating different etiologies and pointing to the need of not mixing them up in a grouped CVD category.