bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Liver Biomarkers in 288 COVID-19 Patients: A Mono-Centric Study in the Early Phase of Pandemic
Authors: Fan Haozhi , Cai Jinyuan , Tian Anran , Li Yuwen , Yuan Hui , Jiang Zhengyi , Yu Yunxi , Ruan Lili , Hu Pingping , Yue Ming , Chen Nian , Li Jun , Zhu Chuanlong
Published: January 2021

Background and Aims: Recent reports have indicated that hepatic dysfunction occurred in a proportion of patients with coronavirus disease 2019 (COVID-19). We aimed to compare and describe the liver biomarkers in different subtypes of COVID-19 patients.

Methods: This study enrolled 288 COVID-19 patients in Huangshi Hospital of Traditional Chinese Medicine. All patients were divided into ordinary, severe, and critical groups according to the Diagnosis and Treatment Protocol for Novel Coronavirus Pneumonia (Trial Version 7). Demographic, clinical characteristics and liver biomarkers were compared among the three groups.

Results: During hospitalization, AST, TBiL, and ALP levels in ordinary and severe patients fluctuated within the normal range with a rising trend in critical patients except AST. ALT and GGT levels fluctuated within the normal range showing an upward trend, while LDH levels in the critical group exceeded the normal range. Prealbumin showed an upward trend, especially in the severe group. At discharge, AST and LDH levels in ordinary and severe groups were lower than their baselines but increased in the critical group. In contrast to albumin, TBiL levels were increased in ordinary and critical groups while decreased in the severe group. The stratified analysis revealed factors affecting liver function in critical cases included highest temperature ≥38.0°C, age ≥60 and symptom of hypoxemia.

Conclusions: COVID-19 can cause severe hepatic dysfunction in critical patients, requiring early monitoring and intervention. LDH, ALP, GGT, TBiL, prealbumin, and albumin may be helpful for evaluating and predicting disease prognosis due to their correlation with disease severity in COVID-19.
Beyond Bone Mineral Density: A New Dual X-Ray Absorptiometry Index of Bone Strength to Predict Fragility Fractures, the Bone Strain Index
Authors: Ulivieri Fabio Massimo , Rinaudo Luca
Published: January 2021

For a proper assessment of osteoporotic fragility fracture prediction, all aspects regarding bone mineral density, bone texture, geometry and information about strength are necessary, particularly in endocrinological and rheumatological diseases, where bone quality impairment is relevant. Data regarding bone quantity (density) and, partially, bone quality (structure and geometry) are obtained by the gold standard method of dual X-ray absorptiometry (DXA). Data about bone strength are not yet readily available. To evaluate bone resistance to strain, a new DXA-derived index based on the Finite Element Analysis (FEA) of a greyscale of density distribution measured on spine and femoral scan, namely Bone Strain Index (BSI), has recently been developed. Bone Strain Index includes local information on density distribution, bone geometry and loadings and it differs from bone mineral density (BMD) and other variables of bone quality like trabecular bone score (TBS), which are all based on the quantification of bone mass and distribution averaged over the scanned region. This state of the art review illustrates the methodology of BSI calculation, the findings of its in reproducibility and the preliminary data about its capability to predict fragility fracture and to monitor the follow up of the pharmacological treatment for osteoporosis.
Effects of Apparent Temperature on the Incidence of Ventricular Tachyarrhythmias in Patients With an Implantable Cardioverter–Defibrillator: Differential Association Between Patients With and Without Electrical Storm
Authors: Huang Hui-Chun , Suen Pei-Chin , Liu Jih-Shin , Chen Cheryl Chia-Hui , Liu Yen-Bin , Chen Chu-Chih
Published: January 2021

Background: Electrical storm (ES) has profound psychological effects and is associated with a higher mortality in patients with implantable cardioverter–defibrillator (ICD). Assessing the incidence and features of ES, is vital. Previous studies have shown winter peaks for ventricular tachyarrhythmia (VTA) in ICD patients. However, the effects of heat with a high relative humidity remain unclear. Thus, this study aimed to assess the nonlinear and lagged effects of apparent temperature [or heat index (HI)] on VTA among patients with and without ES after ICD implantation.

Methods: Of 626 consecutive patients who had ICDs implanted from January 2004 to June 2017 at our hospital, 172 who experienced sustained VTAs in ICD recording were analyzed, and their clinical records were abstracted to assess the association between VTA incidence and HI by time-stratified case-crossover analysis. Cubic splines were used for the nonlinear effect of HI, with adjustment for air pollutant concentrations.

Results: A significant seasonal effect for ES patients was noted. Apparent temperature, but not ambient temperature, was associated with VTA occurrences. The low and high HI thresholds for VTA incidence were <15° and >30°C, respectively, with a percentage change in odds ratios of 1.06 and 0.37, respectively, per 1°C. Lagged effects could only be demonstrated in ES patients, which lasted longer for low HI (in the next 4 days) than high HI (in the next 1 day).

Conclusion: VTA occurrence in ICD patients was strongly associated with low HI and moderately associated with high HI. Lagged effects of HI on VTA were noted in patients with ES. Furthermore, patients with ES were more vulnerable to heat stress than those without ES. Patients with ICD implantation, particularly in those with ES, should avoid exposure to low and high HI to reduce the risk of VTAs, improve quality of life and possibly reduce mortality.
Absence of Uptake and Prion-Like Spreading of Alpha-Synuclein and Tau After Intravitreal Injection of Preformed Fibrils
Authors: Veys Lien , Van houcke Jessie , Aerts Jeroen , Van Pottelberge Sophie , Mahieu Michel , Coens Audrey , Melki Ronald , Moechars Dieder , De Muynck Louis , De Groef Lies
Published: January 2021

Although very different in etiology and symptoms, numerous neurodegenerative diseases can be classified as proteinopathies. More so, evidence indicates that the key misfolded proteins at the basis of different neuropathies might share common mechanisms of propagation. As such, the prion-like spreading of protein aggregates through the neural network is subject of intensive research focus and requires adequate models. Here, we made use of the well-defined architecture and large accessibility of the visual system, of which the retinotopic connections represent a simple route of anterograde signaling and an elegant model to investigate transsynaptic, prion-like spreading. In two independent studies, uptake and seeding of alpha-synuclein and tau were examined after intravitreal injection of preformed fibrils. However, extracellular matrix components in the vitreous space and at the vitreoretinal surface appeared to act as a barrier for the entry of both fibrils into the retina. These results show that further experimental refinement is needed to fully realize the potential of the visual system as a model for studying the molecular and cellular mechanisms of anterograde, transsynaptic spreading of prion-like proteins.
Optimal UAV's Deployment and Transmit Power Design for Two Users Uplink NOMA Systems
Authors: Zhao Fayong
Published: January 2021

In order to fully utilize the spectrum resources, this work considers a unmanned aerial vehicle (UAV) uplink communication system based on non-orthogonal multiple access technology (NOMA), in which the UAV receives information from the ground users with a certain flying altitude. As an initial study, we consider a simplified setup with two ground users to draw some insightful results. Explicitly, we first formulate an optimization problem that maximizes the sum throughput subject to each user's transmit power constraint and their corresponding minimum transmission rate requirement. Then, both the optimal transmit power and UAV's deployment location are derived with the aid of employing the Karush-Kuhn-Tucher (KKT) conditions. Simulation results show that the proposed UAV's deployment scheme with the users' power allocation can achieve a higher sum throughput compared with two existing benchmark schemes.
Nomogram for Stability Stratification of Small Intracranial Aneurysm Based on Clinical and Morphological Risk Factors
Authors: Zhu Wei , Li Wenqiang , Tian Zhongbin , Zhang Mingqi , Zhang Yisen , Wang Kun , Zhang Ying , Yang Xinjian , Liu Jian
Published: January 2021

Background and Purpose: Stability stratification of intracranial aneurysms (IAs) is crucial for individualized clinical management, especially for small IAs. We aim to develop and validate a nomogram based on clinical and morphological risk factors for individualized instability stratification of small IAs.

Methods: Six hundred fifty-eight patients with unstable (n = 293) and stable (n = 416) IAs <7 mm were randomly divided into derivation and validation cohorts. Twelve clinical risk factors and 18 aneurysm morphological risk factors were extracted. Combined with important risk factors, a clinical-morphological predictive nomogram was developed. The nomogram performance was evaluated in the derivation and the validation cohorts in terms of discrimination, calibration, and clinical usefulness.

Results: Five independent instability-related risk factors were included in the nomogram: location, irregularity, side/bifurcation type, flow angle, and height-to-width ratio. In the derivation cohort, the area under the curve (95% CI) of the nomogram was 0.803 (95% CI, 0.764–0.842), and good agreement between predicted instability risk and actual instability status could be detected in the calibration plot. The nomogram also exhibited good discriminations and calibration in the validation cohort: the area under the curve (95% CI) was 0.744 (95% CI, 0.677–0.812). Small IAs with scores <90 were considered to have low risk of instability, and those with scores of 90 or greater were considered to have high risk of instability.

Conclusions: The nomogram based on clinical and morphological risk factors can be used as a convenient tool to facilitate individualized decision-making in the management of small IAs.
Responsive Vagus Nerve Stimulation for Drug Resistant Epilepsy: A Review of New Features and Practical Guidance for Advanced Practice Providers
Authors: Fisher Breanne , DesMarteau Julie A. , Koontz Elizabeth H. , Wilks Seth J. , Melamed Susan E.
Published: January 2021

Vagus nerve stimulation (VNS) is a safe and effective therapy that has been available for over 20 years for adults and children with drug resistant epilepsy (DRE). Since U.S. Food and Drug Administration approval in 1997, VNS has been implanted in over 100,000 patients including over 30,000 children as an adjunctive therapy in reducing the frequency of seizures in patients 4 years of age and older with focal seizures that are refractory to antiseizure medications. VNS Therapy® has evolved over time and currently offers closed-loop, responsive stimulation as well as advanced features that streamline dosing and patient management. Advanced Practice Providers (APPs) such as nurse practitioners, physician assistants and clinical nurse specialists are integral in a comprehensive healthcare team, and dedicated VNS clinics have formed at comprehensive epilepsy centers across the world that are often managed by APPs. This approach improves access, education, and continuity of care for those with VNS or those considering VNS. Here we provide a review for APPs on the VNS Therapy® system focused on new features, dosing, and troubleshooting strategies with the goal to provide guidance to those managing VNS patients.
Numerical Magnitude Affects Accuracy but Not Precision of Temporal Judgments
Authors: Shukla Anuj , Bapi Raju S.
Published: January 2021

A Theory of Magnitude (ATOM) suggests that space, time, and quantities are processed through a generalized magnitude system. ATOM posits that task-irrelevant magnitudes interfere with the processing of task-relevant magnitudes as all the magnitudes are processed by a common system. Many behavioral and neuroimaging studies have found support in favor of a common magnitude processing system. However, it is largely unknown whether such cross-domain monotonic mapping arises from a change in the accuracy of the magnitude judgments or results from changes in precision of the processing of magnitude. Therefore, in the present study, we examined whether large numerical magnitude affects temporal accuracy or temporal precision, or both. In other words, whether numerical magnitudes change our temporal experience or simply bias duration judgments. The temporal discrimination (between comparison and standard duration) paradigm was used to present numerical magnitudes (“1,” “5,” and “9”) across varied durations. We estimated temporal accuracy (PSE) and precision (Weber ratio) for each numerical magnitude. The results revealed that temporal accuracy (PSE) for large (9) numerical magnitude was significantly lower than that of small (1) and identical (5) magnitudes. This implies that the temporal duration was overestimated for large (9) numerical magnitude compared to small (1) and identical (5) numerical magnitude, in line with ATOM’s prediction. However, no influence of numerical magnitude was observed on temporal precision (Weber ratio). The findings of the present study suggest that task-irrelevant numerical magnitude selectively affects the accuracy of processing of duration but not duration discrimination itself. Further, we argue that numerical magnitude may not directly affect temporal processing but could influence via attentional mechanisms.
Effectiveness of Postoperative Adjuvant Radiotherapy in Atypical Meningioma Patients After Gross Total Resection: A Meta-Analysis Study
Authors: He Lingzhe , Zhang Buyi , Zhang Jianmin , Guo Zhige , Shi Feina , Zeng Qiang
Published: January 2021

Background

It still remains unclear whether patients with atypical meningioma (AM) could benefit from postoperative adjuvant radiotherapy (PORT) after gross-total resection (GTR).

Objective

Exploring the effectiveness of PORT on AM patients after GTR.

Methods

Literatures on PubMed, Embase, Web of science, and Scopus databases published between January 2000 and January 2019 were searched. After the selection based on the certain exclusion criteria, the Newcastle-Ottawa evaluation scale was used to evaluate the quality of the included literatures. Finally, a meta-analysis was conducted to analyze the effectiveness of PORT on local control (LC), progression-free survival (PFS) and overall survival (OS) in atypical meningioma patients after GTR.

Results

A total of 17 articles with 2,008 AM patients were included in the meta-analysis. The 5-year LC, 5-year PFS, and 5-year OS rates were 82.2, 84.1, and 79.0%, respectively, for AM patients receiving PORT after GTR, and they were 71.0, 71.9, and 81.5%, respectively, for those not receiving PORT after GTR. PORT could significantly improve 5-year LC rate (OR [95% Cl] = 2.59 [1.40–4.81], P = 0.002) and 5-year PFS rate (OR [95% Cl] = 1.99 [1.35–2.95], P = 0.001), but did not significantly improve 5-year OS rate (OR [95% Cl] = 1.07 [0.60–1.91], P = 0.828).

Conclusion

PORT could improve the 5-year LC rate and 5-year PFS rate in AM patients after GTR. AM patients might benefit from PORT after GTR.
Lobular Breast Cancer: A Review
Authors: Wilson Natalie , Ironside Alastair , Diana Anna , Oikonomidou Olga
Published: January 2021

Invasive lobular carcinoma accounts for 5%–15% of all invasive breast cancers, with a marked increase in incidence rates over the past two decades. Distinctive biological hallmarks of invasive lobular carcinoma include the loss of cell adhesion molecule E-cadherin leading to cells with a discohesive morphology, proliferating into single-file strands and estrogen receptor positivity. These key molecular features can make diagnosis difficult, as invasive lobular carcinoma is challenging to detect both physically and with current standard imaging. Treatment of invasive lobular carcinoma strongly favors endocrine therapy due to low chemosensitivity and lower rates of pathological response as a result. This review will summarize the distinct biological and molecular features of invasive lobular carcinoma, focusing on the diagnostic challenges faced and the subsequent surgical and medical management strategies. Prospective therapeutic options will also be explored, highlighting how furthering our understanding of the unique biology of lobular breast carcinoma is essential in guiding and informing the treatment of patients in the future.
Detection of Low-Frequency KRAS Mutations in cfDNA From EGFR-Mutated NSCLC Patients After First-Line EGFR Tyrosine Kinase Inhibitors
Authors: Nardo Giorgia , Carlet Jessica , Marra Ludovica , Bonanno Laura , Boscolo Alice , Dal Maso Alessandro , Boscolo Bragadin Andrea , Indraccolo Stefano , Zulato Elisabetta
Published: January 2021

Background

Molecular profiling of advanced EGFR mutated NSCLC has recently demonstrated the co-existence of multiple genetic alterations. Specifically, co-existing KRAS-mutations in EGFR NSCLCs have been described, despite their prevalence at progression and their role in the response to EGFR tyrosine kinase inhibitors (TKIs) remain marginally explored. Aim of our study was to investigate the prevalence of co-existing KRAS mutations at the time of progressive disease and explore their impact on clinical outcome.

Materials and Methods

We retrospectively analyzed by digital droplet PCR prevalence of KRAS co-mutations in 106 plasma samples of EGFR mutated NSCLC patients, in progressive disease after EGFR TKI treatment as first-line therapy.

Results

KRAS co-mutations (codon 12 and 13) were identified in 3 patients (2.8% of analyzed samples), with low allelic frequency (<0.2%), and had a negative impact on clinical outcome to first-line EGFR TKI.

Conclusion

Detection of KRAS mutations in cell-free DNA of EGFR mutant NSCLC patients at progression after first or second generation EGFR TKI is a rare event. Due to their low abundance, the negative impact of KRAS mutations on the response to EGFR TKI remains to be confirmed in larger studies.
Development of a Perinatal Palliative Care Model at a Level II Perinatal Center Supported by a Pediatric Palliative Care Network
Authors: Bolognani Marco , Morelli Paola Daniela , Scolari Isabella , Dolci Cristina , Fiorito Valentina , Uez Francesca , Graziani Silvia , Stefani Barbara , Zeni Francesca , Gobber Gino , Bravi Elena , Tateo Saverio , Soffiati Massimo
Published: January 2021

Objective: To describe the model build up to take care of fetuses and newborns eligible to perinatal palliative care (PnPC) followed in an Italian II level perinatal center.

Methods: Retrospective chart review of all fetuses and newborn infants eligible to PnPC admitted to level II perinatal center within a 4 years period.

Results: Forty-five of 848 infants (0.5%) referred to II level NICU were eligible to PnPC. Twenty-seven percentage had fetal diagnosis. Twenty percentage were preterm infants at the limit of viability, 35% were newborns with life limiting or life threatening disease diagnosed in utero or at the postnatal ward, 45% were newborns not responding to intensive care intervention with high health care needs or medical complexity. Fifty-seven percentage of neonates admitted to NICU died before discharge, while 16 (35% of population considered) were discharged home. Median age at death was 4 days after birth, and delivery room death immediately after birth occurred in six patients (13%).

Conclusions: Despite the paucity of our population and the high variability in disease trajectories the perinatal palliative care program build up in our region provides a reproducible method for a structured taking in charge of fetuses and neonates eligible to PnPC and their families, from the time of diagnosis to bereavement, in both outpatient and inpatient settings.
Not All Fibers Are Born Equal; Variable Response to Dietary Fiber Subtypes in IBD
Authors: Armstrong Heather , Mander Inderdeep , Zhang Zhengxiao , Armstrong David , Wine Eytan
Published: January 2021

Diet provides a safe and attractive alternative to available treatment options in a variety of diseases; however, research has only just begun to elucidate the role of diet in chronic diseases, such as the inflammatory bowel diseases (IBD). The chronic and highly debilitating IBDs, Crohn disease and ulcerative colitis, are hallmarked by intestinal inflammation, immune dysregulation, and dysbiosis; and evidence supports a role for genetics, microbiota, and the environment, including diet, in disease pathogenesis. This is true especially in children with IBD, where diet-based treatments have shown excellent results. One interesting group of dietary factors that readily links microbiota to gut health is dietary fibers. Fibers are not digested by human cells, but rather fermented by the gut microbes within the bowel. Evidence has been mounting over the last decade in support of the importance of dietary fibers in the maintenance of gut health and in IBD; however, more recent studies highlight the complexity of this interaction and importance of understanding the role of each individual dietary fiber subtype, especially during disease. There are roughly ten subtypes of dietary fibers described to date, categorized as soluble or insoluble, with varying chemical structures, and large differences in their fermentation profiles. Many studies to date have described the benefits of the byproducts of fermentation in healthy individuals and the potential health benefits in select disease models. However, there remains a void in our understanding of how each of these individual fibers affect human health in dysbiotic settings where appropriate fermentation may not be achieved. This review highlights the possibilities for better defining the role of individual dietary fibers for use in regulating inflammation in IBD.
Timosaponin AIII Induces G2/M Arrest and Apoptosis in Breast Cancer by Activating the ATM/Chk2 and p38 MAPK Signaling Pathways
Authors: Zhang Minjie , Qu Jiaxi , Gao Zhiwei , Qi Qi , Yin Hong , Zhu Ling , Wu Yichen , Liu Wei , Yang Jian , Huang Xuefeng
Published: January 2021

Timosaponin AIII (TAIII), a steroidal saponin, exerts potent anti-tumor activity in various cancers, especially breast cancer. However, the concrete molecular mechanisms of TAIII against breast cancer are still unclear. Here, we find that TAIII triggers DNA damage, leads to G2/M arrest, and ultimately induces apoptosis in breast cancer both in vitro and in vivo. TAIII induced G2/M phase arrest and apoptosis in MDA-MB-231 and MCF7 cells accompanied with down-regulation of CyclinB1, Cdc2 and Cdc25C. Further data showed that the ATM/Chk2 and p38 pathways were activated representing by up-regulated levels of p-H2A.X and p-p38, which indicated an induction of DNA damage by TAIII, leading to cell cycle arrest and apoptosis. The effects of TAIII were further confirmed by employing inhibitors of ATM and p38 pathways. In vivo, TAIII suppressed the growth of subcutaneous xenograft tumor without obvious toxicity, which indicated by Ki67 and TUNEL analysis. Data also showed that TAIII stimulated the ATM/Chk2 and p38 MAPK pathways in vivo, which in consistent with the effects in vitro. Hence, our data demonstrate that TAIII triggers DNA damage and activates ATM/Chk2 and p38 MAPK pathways, and then induces G2/M phase arrest and apoptosis in breast cancer, which provide theoretical evidence for TAIII utilized as drug against breast cancer.
A New Detection Method Defining the Aerobic Threshold for Endurance Exercise and Training Prescription Based on Fractal Correlation Properties of Heart Rate Variability
Authors: Rogers Bruce , Giles David , Draper Nick , Hoos Olaf , Gronwald Thomas
Published: January 2021

The short-term scaling exponent alpha1 of detrended fluctuation analysis (DFA a1), a nonlinear index of heart rate variability (HRV) based on fractal correlation properties, has been shown to steadily change with increasing exercise intensity. To date, no study has specifically examined using the behavior of this index as a method for defining a low intensity exercise zone. The aim of this report is to compare both oxygen intake (VO2) and heart rate (HR) reached at the first ventilatory threshold (VT1), a well-established delimiter of low intensity exercise, to those derived from a predefined DFA a1 transitional value. Gas exchange and HRV data were obtained from 15 participants during an incremental treadmill run. Comparison of both VO2 and HR reached at VT1 defined by gas exchange (VT1 GAS) was made to those parameters derived from analysis of DFA a1 reaching a value of 0.75 (HRVT). Based on Bland Altman analysis, linear regression, intraclass correlation (ICC) and t testing, there was strong agreement between VT1 GAS and HRVT as measured by both HR and VO2. Mean VT1 GAS was reached at 39.8 ml/kg/min with a HR of 152 bpm compared to mean HRVT which was reached at 40.1 ml/kg/min with a HR of 154 bpm. Strong linear relationships were seen between test modalities, with Pearson’s r values of 0.99 (p < 0.001) and.97 (p < 0.001) for VO2 and HR comparisons, respectively. Intraclass correlation between VT1 GAS and HRVT was 0.99 for VO2 and 0.96 for HR. In addition, comparison of VT1 GAS and HRVT showed no differences by t testing, also supporting the method validity. In conclusion, it appears that reaching a DFA a1 value of 0.75 on an incremental treadmill test is closely associated with crossing the first ventilatory threshold. As training intensity below the first ventilatory threshold is felt to have great importance for endurance sport, utilization of DFA a1 activity may provide guidance for a valid low training zone.
Calcium-Sensing Receptor Participates in High Glucose-Induced EndMT in Primary Human Aortic Endothelial Cells
Authors: Yuan Cheng , Ni Lihua , Yang Xianqin , Zhang Changjiang , Wu Xiaoyan
Published: January 2021

Objective

Previous studies have shown that high glucose (HG) induces endothelial cell (EC) damage via endothelial-to-mesenchymal transition (EndMT). Although the underlying mechanisms are still unclear, recent studies have demonstrated the role of calcium-sensing receptor (CaSR) in mediating EC damage. Therefore, the aim of our study was to investigate whether CaSR mediates HG-induced EndMT and to determine the underlying mechanism.

Methods

Bioinformatics analysis of microarray profiles (GSE30780) and protein-protein interaction (PPI) analyses were performed to select the hub genes. As for in vitro research, the human aortic ECs (HAECs) were exposed to HG to induce EndMT. The expression of CaSR and β-catenin was determined, as well as their effects on EndMT (endothelial marker CD31, mesenchymal marker FSP1, and α-SMA).

Results

The bioinformatics analysis indicated CaSR was significantly increased in HG-treated HAECs and was one of the hub genes. The in vitro results showed that HG significantly inhibited the expression of CD31 and increased FSP1 and α-SMA in a concentration- and time-dependent manner. Moreover, CaSR was increased in HAECs after HG treatment. The CaSR antagonist attenuated HG-induced expression of EndMT-related markers. Furthermore, HG treatment increased the nuclear translocation of β-catenin in HAECs. In contrast, blocking the nuclear translocation of β-catenin by DKK1 could attenuate HG-induced EndMT (increased the protein expression of CD31 by 30% and decreased the protein expression of FSP1 by 15% and α-SMA by 25%). CaSR siRNA further inhibited the HG-induced nuclear translocation of β-catenin in HAECs.

Conclusion

Our research demonstrated that HG-induced EndMT in HAECs might be mediated by CaSR and the downstream nuclear translocation of β-catenin.
Depression, Anxiety and Post-traumatic Growth Among Bereaved Adults: A Latent Class Analysis
Authors: Li Jie , Sun Yihua , Maccallum Fiona , Chow Amy Y. M.
Published: January 2021

Background

The death of a loved one can trigger a range of responses, including painful thoughts and emotions, as well as positive changes, such as post-traumatic growth (PTG). To understand more about the relationship between these outcomes this study explored the co-occurrence of depression, anxiety and PTG among a group of bereaved Chinese adults.

Methods

Data were collected from 194 participants, who had lost a first-degree relative. Latent class analysis was used to analyze the data to identify subgroups of participants with shared symptom profiles.

Results

Three classes were identified: a Growth class, a Depression/Anxiety/Growth class and a Depression/Anxiety class. Marital status, social support, close relationship with the deceased and witnessing the suffering of the deceased were identified as differential predictors of class membership.

Conclusion

The findings contribute to our understanding of the potentially wide ranging impacts of bereavement and highlight the important role of stressor characteristics and support and in influencing impairment and positive outcomes.
Using Signal Detection Theory to Better Understand Cognitive Fatigue
Authors: Wylie Glenn R. , Yao Bing , Sandry Joshua , DeLuca John
Published: January 2021

When we are fatigued, we feel that our performance is worse than when we are fresh. Yet, for over 100 years, researchers have been unable to identify an objective, behavioral measure that covaries with the subjective experience of fatigue. Previous work suggests that the metrics of signal detection theory (SDT)—response bias (criterion) and perceptual certainty (d’)—may change as a function of fatigue, but no work has yet been done to examine whether these metrics covary with fatigue. Here, we investigated cognitive fatigue using SDT. We induced fatigue through repetitive performance of the n-back working memory task, while functional magnetic resonance imaging (fMRI) data was acquired. We also assessed cognitive fatigue at intervals throughout. This enabled us to assess not only whether criterion and d’ covary with cognitive fatigue but also whether similar patterns of brain activation underlie cognitive fatigue and SDT measures. Our results show that both criterion and d’ were correlated with changes in cognitive fatigue: as fatigue increased, subjects became more conservative in their response bias and their perceptual certainty declined. Furthermore, activation in the striatum of the basal ganglia was also related to cognitive fatigue, criterion, and d’. These results suggest that SDT measures represent an objective measure of cognitive fatigue. Additionally, the overlap and difference in the fMRI results between cognitive fatigue and SDT measures indicate that these measures are related while also separate. In sum, we show the relevance of SDT measures in the understanding of fatigue, thus providing researchers with a new set of tools with which to better understand the nature and consequences of cognitive fatigue.
Detection of Genuine and Posed Facial Expressions of Emotion: Databases and Methods
Authors: Jia Shan , Wang Shuo , Hu Chuanbo , Webster Paula J. , Li Xin
Published: January 2021

Facial expressions of emotion play an important role in human social interactions. However, posed expressions of emotion are not always the same as genuine feelings. Recent research has found that facial expressions are increasingly used as a tool for understanding social interactions instead of personal emotions. Therefore, the credibility assessment of facial expressions, namely, the discrimination of genuine (spontaneous) expressions from posed (deliberate/volitional/deceptive) ones, is a crucial yet challenging task in facial expression understanding. With recent advances in computer vision and machine learning techniques, rapid progress has been made in recent years for automatic detection of genuine and posed facial expressions. This paper presents a general review of the relevant research, including several spontaneous vs. posed (SVP) facial expression databases and various computer vision based detection methods. In addition, a variety of factors that will influence the performance of SVP detection methods are discussed along with open issues and technical challenges in this nascent field.
Comparing the Bayesian Unknown Change-Point Model and Simulation Modeling Analysis to Analyze Single Case Experimental Designs
Authors: Natesan Batley Prathiba , Nandakumar Ratna , Palka Jayme M. , Shrestha Pragya
Published: January 2021

Recently, there has been an increased interest in developing statistical methodologies for analyzing single case experimental design (SCED) data to supplement visual analysis. Some of these are simulation-driven such as Bayesian methods because Bayesian methods can compensate for small sample sizes, which is a main challenge of SCEDs. Two simulation-driven approaches: Bayesian unknown change-point model (BUCP) and simulation modeling analysis (SMA) were compared in the present study for three real datasets that exhibit “clear” immediacy, “unclear” immediacy, and delayed effects. Although SMA estimates can be used to answer some aspects of functional relationship between the independent and the outcome variables, they cannot address immediacy or provide an effect size estimate that considers autocorrelation as required by the What Works Clearinghouse (WWC) Standards. BUCP overcomes these drawbacks of SMA. In final analysis, it is recommended that both visual and statistical analyses be conducted for a thorough analysis of SCEDs.
Autism Spectrum Disorder Symptom Profile Across the RASopathies
Authors: Geoffray Marie-Maude , Falissard Bruno , Green Jonathan , Kerr Browyn , Evans D. Gareth , Huson Susan , Burkitt-Wright Emma , Garg Shruti
Published: January 2021

Dysregulation of the Ras MAPK signaling pathway is implicated in the pathogenesis of autism spectrum disorder (ASD). The RASopathies, a group of disorders caused by mutations of the Ras/MAPK pathway genes, share many overlapping clinical features. Studies suggest a high prevalence of ASD in the RASopathies, but detailed characterization of the ASD profile is lacking. The aim of this study was to compare the ASD symptom profile of three distinct RASopathies associated with both gain-of-function and loss-of-function mutations: neurofibromatosis type 1 (NF1), Noonan syndrome (NS), and cardiofaciocutaneous syndrome (CFC). Participants were drawn from existing databases if they had a diagnosis of a RASopathy, met the criteria for ASD, and were able to communicate verbally. We compared the phenotypic profile of NF1 + ASD (n = 48), NS + ASD (n = 11), and CFC + ASD (n = 7) on the Autism Diagnostic Inventory (ADI-R) and the Autism Diagnostic Observation Schedule (ADOS). We found subtle but non-significant group differences with higher levels of social impairments and lower restricted repetitive behaviors in the NF1 group as compared with the NS and CFC groups. We observed group differences in developmental milestones with most severe delays in CFC, followed by NS and NF1. Our results suggest that despite developmental differences, the ASD profile remains relatively consistent across the three RASopathies. Though our results need confirmation in larger samples, they suggest the possibility that treatment and mechanistic insights developed in the context of one RASopathy may be generalizable to others and possibly to non-syndromic ASD associated with dysregulation of Ras/MAPK pathway genes.
Physical Activity Intensity Cut-Points for Wrist-Worn GENEActiv in Older Adults
Authors: Fraysse François , Post Dannielle , Eston Roger , Kasai Daiki , Rowlands Alex V. , Parfitt Gaynor
Published: January 2021

Purpose: This study aims to (1) establish GENEActiv intensity cutpoints in older adults and (2) compare the classification accuracy between dominant (D) or non-dominant (ND) wrist, using both laboratory and free-living data.

Methods: Thirty-one older adults participated in the study. They wore a GENEActiv Original on each wrist and performed nine activities of daily living. A portable gas analyzer was used to measure energy expenditure for each task. Testing was performed on two occasions separated by at least 8 days. Some of the same participants (n = 13) also wore one device on each wrist during 3 days of free-living. Receiver operating characteristic analysis was performed to establish the optimal cutpoints.

Results: For sedentary time, both dominant and non-dominant wrist had excellent classification accuracy (sensitivity 0.99 and 0.97, respectively; specificity 0.91 and 0.86, respectively). For Moderate to Vigorous Physical Activity (MVPA), the non-dominant wrist device had better accuracy (ND sensitivity: 0.90, specificity 0.79; D sensitivity: 0.90, specificity 0.64). The corresponding cutpoints for sedentary-to-light were 255 and 375 g · min (epoch independent: 42.5 and 62.5 mg), and those for the light-to-moderate were 588 and 555 g · min (epoch-independent: 98.0 and 92.5 mg) for the non-dominant and dominant wrist, respectively. For free-living data, the dominant wrist device resulted in significantly more sedentary time and significantly less light and MVPA time compared to the non-dominant wrist.
Game Insight Skills as a Predictor of Talent for Youth Soccer Players
Authors: de Joode Tom , Tebbes Drewes J. J. , Savelsbergh Geert J. P.
Published: January 2021

Perceptual–cognitive skills are found to be important factors for soccer players. The aim of this study was, therefore, to find within-group differences for game insight in an elite group of youth soccer players by means of a Game Insight inDicator (GID). In addition, the prospective value of perceptual–cognitive skills was examined by following the trajectory of the participants. The GID consisted of film clips that show game situations. The task of the players was to predict the trajectory and destination of the ball and move toward the correct position to receive the pass of a teammate. The film clips stopped 80 ms before, at, and 80 ms after the football contact of a teammate. We also sought to validate the GID against game performance. Participants were talented soccer players 11–13 years old and playing at the elite level for their age. Based on eight independent elite-coach judgments, two groups were created: highly talented players (HT) and less talented players (LT). The coach ratings were supported by a significant difference between the two groups based on the objective notational analysis of their game performance in 4 vs. 4 and 11 vs. 11 matches. With respect to the GID, a significant interaction effect for the groups (HT vs. LT) by occlusion time (−80, 0, and +80 ms) was found, showing that the HT performs better than the LT in 0 and +80 ms condition. In addition, GID scores were compared with soccer levels at the mean age of 19 years. Longitudinal data did not show significant differences between elite and sub-elite. Overall, the GID was found to be a valid and useful indicator for players anticipating the ball's trajectory and destination at age 11–13 years but failed to predict the players' level at age 19 years. The latter indicates how difficult it is to predict talent development.
Proximal Trauma Increases Risk of Venous Thrombosis in Soft Tissue Reconstruction of Open Lower Limb Fractures
Authors: Yalcin Nilay G. , Bruscino-Raiola Frank , Ferris Scott
Published: January 2021

Lower limb salvage after major trauma is a complex undertaking. For patients who have suffered multi-level trauma to their lower limb we postulated that pelvic injury or ipsilateral lower limb injury proximal to the site of a free flap may increase the rate of post-operative complications. All patients who underwent lower limb free flap reconstruction as a result of acute trauma between January 2010 and December 2017 were included. The patients were divided into the study group (50 patients), who sustained a lower limb or pelvic injury proximal to the free flap site, and control group (91 patients) who did not sustain proximal lower limb or pelvic trauma. Complication rates were compared between the two groups. Overall, the proximal trauma group anastomotic thrombosis rate of 18.0% was significantly higher than the control group thrombosis rate of 2.2%. There was no statically significant difference in rates of hematoma, swelling or infection. Flap loss rate in the proximal trauma group was 4.0%, compared to the control group at 2.2%. All patients with a failed flap went onto have a successful reconstruction with a subsequent flap in the acute admission and there were no amputations. In the proximal injury study group despite the significantly increased rate of microvascular thrombosis requiring revision, the ultimate primary free flap survival rate was still 96%. Overall, severe coexisting proximal trauma predicted a higher venous microvascular complication rate but was not a contraindication to limb salvage.
Variability in Human-Animal Interaction Research
Authors: Rodriguez Kerri E. , Herzog Harold , Gee Nancy R.
Published: January 2021

The field of Human-Animal Interaction (HAI) is plagued with mixed results. Some findings appear to indicate that interacting with a companion animal is beneficial for some aspect of human health and well-being, while other research outcomes are inconclusive or even indicate the opposite. The purpose of this paper is to take a closer look at this variability in research outcomes and to provide plausible explanations and potential remedies. Some of the reasons for mixed results are likely due to the wide variety of methodologies implemented, intermittent use of standardized measures and manualized protocols, variability in human and animal participants, and limited quantification of human-animal interactions or definitions of pet ownership. Variability in research outcomes is not unique to HAI and is, in fact, not uncommon in many more established fields such as psychology and medicine. However, the potential reasons for the variability may be linked to the unique nature of HAI in that, in its' simplest form, it involves two complex organisms, a human and an animal, interacting in dynamic ways. We argue that this complexity makes research in this field particularly challenging and requires a broad spectrum of theoretical and methodological considerations to improve rigor while ensuring the validity and reliability of conclusions drawn from study results.
Educational Methods of Engineering Training
Authors: Bagyinszki Gyula , Bitay Enikő
Published: January 2021

The choice and the appropriate combination of methods used in the teaching-learning (education – knowledge-acquisition) process in technical higher education depends to a large extent on the personality of the lecturer, his/her pedagogical culture, personal competences and of course, the technical possibilities at his/ her disposal. This study provides a concise overview of the methods as tried-and-tested systems of consciously implemented operations and means that are systematically used by the lecturer to achieve the objectives of training in practical cooperation with the students.
Innovation Design for Safe Products
Authors: Csongvai Roland , Torkos Zoltán
Published: January 2021

Innovation & product safety in the case of the EC & Hungarian SMEs is a top priority, where product safety is also a legal obligation. Despite this, innovation design is dealt with inadequately by the majority of companies, and this is due to lack of knowledge or competency, especially considering the processes related to the design of safe products. Improper behaviour in the design stages results in losses for the companies - losses due to the inadequacy of otherwise innovative products. Inadequate or poorly applied directives/policies, especially when coded into processes influence not only the safety of the products but can result in delays to market access, significantly increasing costs & development time, as well jeopardising the product’s acceptance on the future market.
A Cost Effective Rapid Weld Technology Configuration
Authors: Laki Márk , Kuti János
Published: January 2021

The research focuses on determining the application parameters of a new process developed by Cloos. With the use of Cloos Rapid Weld technology (metal active gas welding), the authors carried out welding experiments on S235JR steel sheets (6 mm and 8 mm thick). The results of the welding experiments were verified by hardness measurement [1–3]. The goal is to establish one side one row suitable welded joint by the used technology.
Investigations of Detergents and Metal Components for Supporting the Effective Treatment of Greywater
Authors: Lós Réka , IzbékinéSzabolcsik Andrea , Bodnár Ildikó
Published: January 2021

The aim of our research was to investigate the detergent content and several micro and macro elements in greywater samples, a necessary step in determining the water quality for the further reuse of the greywater. We determined the anionic detergent content of synthetic greywater with a two-phased titration method prepared in laboratory conditions. The current study leads to a new suggestion for a more accurate and reliable titrimetric method for determination of anionic detergent content of synthetic greywater samples. Following these investigations, we also examined the metal content of synthetic bath water. We wanted to highlight how detergents in greywater influence the micro and macro element content of the given synthetic bath water samples.
The Impact of Active Cooling on Heat Resistant PLA
Authors: Lukács Norbert László , Ficzere Péter , Szebényi Gábor
Published: January 2021

In this study the properties of HT-PLA were determined with tensile tests. The influence of cooling was determined and influence of manufacturing environment shown to be predictable. Heat resistant 3D printing materials can be widely used in the manufacturing process but these materials are relatively expensive. HTPLA is one of the cheapest materials with these parameters.
Numerical Modelling of the Three-Roll Bending Process of a Thin Plate
Authors: Máté Péter , Szekrényes András
Published: January 2021

The three-roll bending process is a simple procedure, commonly used in the industry, through which a cylindrical surface can be produced from a sheet plate. This process is mainly controlled through experience and it is described with the finite element method, except for a very few numerical and analytical investigations. The topic of this article is to present a numerical method, through which the curvature function along the rolling direction can be calculated. This article presents the proposed numerical method and its verification with the finite element method. The results of the two numerical methods are in good agreement.