bluuebunny/crossref_metadata_2025 huggingface dataset:
Semantic Segmentation of 3D Point Cloud Based on Spatial Eight-Quadrant Kernel Convolution
Authors: Liu Liman , Yu Jinjin , Tan Longyu , Su Wanjuan , Zhao Lin , Tao Wenbing
Published: August 2021

In order to deal with the problem that some existing semantic segmentation networks for 3D point clouds generally have poor performance on small objects, a Spatial Eight-Quadrant Kernel Convolution (SEQKC) algorithm is proposed to enhance the ability of the network for extracting fine-grained features from 3D point clouds. As a result, the semantic segmentation accuracy of small objects in indoor scenes can be improved. To be specific, in the spherical space of the point cloud neighborhoods, a kernel point with attached weights is constructed in each octant, the distances between the kernel point and the points in its neighborhood are calculated, and the distance and the kernel points’ weights are used together to weight the point cloud features in the neighborhood space. In this case, the relationship between points are modeled, so that the local fine-grained features of the point clouds can be extracted by the SEQKC. Based on the SEQKC, we design a downsampling module for point clouds, and embed it into classical semantic segmentation networks (PointNet++, PointSIFT and PointConv) for semantic segmentation. Experimental results on benchmark dataset ScanNet V2 show that SEQKC-based PointNet++, PointSIFT and PointConv outperform the original networks about 1.35–2.12% in terms of MIoU, and they effectively improve the semantic segmentation performance of the networks for small objects of indoor scenes, e.g., the segmentation accuracy of small object “picture” is improved from 0.70% of PointNet++ to 10.37% of SEQKC-PointNet++.
Privacy-Preserving and Lightweight Selective Aggregation with Fault-Tolerance for Edge Computing-Enhanced IoT
Authors: Wang Qiannan , Mu Haibing
Published: August 2021

Edge computing has been introduced to the Internet of Things (IoT) to meet the requirements of IoT applications. At the same time, data aggregation is widely used in data processing to reduce the communication overhead and energy consumption in IoT. Most existing schemes aggregate the overall data without filtering. In addition, aggregation schemes also face huge challenges, such as the privacy of the individual IoT device’s data or the fault-tolerant and lightweight requirements of the schemes. In this paper, we present a privacy-preserving and lightweight selective aggregation scheme with fault tolerance (PLSA-FT) for edge computing-enhanced IoT. In PLSA-FT, selective aggregation can be achieved by constructing Boolean responses and numerical responses according to specific query conditions of the cloud center. Furthermore, we modified the basic Paillier homomorphic encryption to guarantee data privacy and support fault tolerance of IoT devices’ malfunctions. An online/offline signature mechanism is utilized to reduce computation costs. The system characteristic analyses prove that the PLSA-FT scheme achieves confidentiality, privacy preservation, source authentication, integrity verification, fault tolerance, and dynamic membership management. Moreover, performance evaluation results show that PLSA-FT is lightweight with low computation costs and communication overheads.
Opportunities for and Limits to Cooperation between School and Families in Sustainable Public Food Procurement
Authors: Pagliarino Elena , Santanera Elena , Falavigna Greta
Published: August 2021

This paper describes a research project, carried out in an Italian public school, to assess whether parents were willing to take part in food procurement decisions, as well as their ability to accurately predict what foods children would pick at school lunch and their propensity to support sustainable food choices made by the school. The methodology included a questionnaire, issued to 500 parents, and an in-depth study of 138 child/parent pairs. The study comprised: (i) presentation of an innovative recipe in the weekly menu of the school canteen; (ii) meal observations of children’s intake at school lunch during the week of the menu modification; (iii) collection of both parents’ and children’s reports on their choices of recipes from the modified weekly menu. The results are commented in light of two important changes that have recently affected Italian public school food procurement: the opening of school canteens to lunches brought from home and the measures adopted since 2020 to contain the COVID-19 pandemic. Both events go in the direction of delegating to parents the multifaceted role of the school in the food arena. The article concludes that the results of the study should discourage this approach.
Circular Construction Process: Method for Developing a Selective, Low CO2eq Disassembly and Demolition Plan
Authors: Melella Roberta , Di Ruocco Giacomo , Sorvillo Alfonso
Published: August 2021

With the increasing focus on the construction sector (e.g., following the European Green Deal initiative) with the aim to reduce emissions by 55% by 2030 (compared to 1990 levels), as well as achieve full decarbonisation by 2050, the built environment remains a strategic domain for the R&I (Research and Innovation) agenda. Indeed, the building and construction sector is the main contributor to greenhouse gas emissions (39% of global emissions as of 2018), highlighting the need to start a process of decarbonisation of this sector. The overall reduction in the environmental impact of building materials is achieved by establishing sustainable continuity between the end-of-life phase of the building and the production phase of individual building components. In particular, with reference to the end-of-life phase of the building (BS EN 15978: 2011), the Minimum Environmental Criteria foresee the preparation of a plan for the disassembly and selective demolition of the building, which allows the reuse or recycling of materials, building components and prefabricated elements used. According to the guidelines of a low-carbon construction design, which takes into account a circular economy, the following thesis deals with a methodological proposal to study “dry” construction systems (wood and steel). In particular, the study intends to reach the development of such an elaboration by carrying out an assessment of the environmental impact of a process of selective disassembly and demolition of steel building systems. The model is developed on the basis of a reading of the level of sustainability of emblematic case studies, appropriately identified, i.e., ‘quality’ architectures, built with ‘dry’ (steel) building systems.
Development and Verification of the Effectiveness of a Fine Dust Reduction Planting Model for Socially Vulnerable Area
Authors: Choi YunEui , Ji Eunhye , Chon Jinhyung
Published: August 2021

Creating a green infrastructure that is effective for reducing fine dust is a significant challenge for urban landscape planners. In this study, a fine dust reduction planting model that can be applied to socially vulnerable area was developed, and its effects were verified. Using PM10, PM2.5, temperature, relative humidity, wind direction, and wind speed measured for approximately one year, the changes in the concentration of fine dust according to the weather conditions were investigated. As a result of the analysis, there was a significant difference in the concentration of fine dust inside and outside the planting zone (p < 0.05). In addition, there is a significant difference between the fine dust reduction effect of the multilayered planting model and the single planting model (p < 0.05). The paper’s main findings are as follows: (1) When the green cover rate is over 50%, the concentration of fine dust is lower than that outside the planting zones. (2) Multilayered planting zones are more effective in reducing the concentration of fine dust than single-structured planting zones. (3) Multilayered planting zones reduce the concentration of fine dust by changing the microclimate. The results of this study can be used as basic data for small urban planting design to reduce fine dust for children’s health in socially vulnerable areas.
Structure and Properties of New Antifriction Composites Based on Tool Steel Grinding Waste
Authors: Roik Tetiana , Rashedi Ahmad , Khanam Taslima , Chaubey Abhay , Balaganesan Gurusami , Ali Sadaqat
Published: August 2021

This article investigates the impact of manufacturing technology on the structure, mechanical, and tribological properties of new antifriction composite materials based on R6M5 high-speed tool steel grinding waste. The characteristics of the new composite’s structure formation and its impact on properties after use of the established technological modes, including grinding waste regeneration, were illustrated. It was demonstrated that such technology is capable of ensuring microheterogeneous structure. The material’s structure consists of the metal matrix based on R6M5 high-speed tool steel waste and uniformly distributed CaF2 solid lubricant in the steel matrix. As compared to known iron-based composites, this structure promotes a high degree of mechanical and tribological properties. During tribological tests, anti-seize thin films of 15–20 μm are formed on the contacting surfaces. These constantly renewable films contribute to the high antifriction properties of the composite under the studied friction conditions and provide a self-lubricating effect. Such films fully cover both the material’s surface and the counterface. The formation of antifriction films results in the self-lubrication mode. The findings of the study open up the possibility of predicting the friction behavior of a composite at high temperatures by selecting the initial metal grinding waste to ensure the appropriate level of properties. The extensive use of various alloy steel-based industrial grinding waste in the re-production cycle would significantly contribute to resolving the global environmental problem of protecting the environment from pollution.
Utilization of Municipal Solid Waste Incineration Bottom Ash as Fine Aggregate of Cement Mortars
Authors: Woo Byeong-Hun , Jeon In-Kyu , Yoo Dong-Ho , Kim Seong-Soo , Lee Jeong-Bae , Kim Hong-Gi
Published: August 2021

Incineration bottom ash is generated by the incineration of solid waste. Household solid waste is increasing every year and so is incineration bottom ash. This is a problem to treat the incineration bottom ash because the ash has many toxic components. Cement composites can solve this problem and there are many studies for using the bottom ash as fine aggregate. To evaluate the usage of incineration bottom ash, compressive strength, mercury intrusion porosimetry, scanning electron microscopy-backscatter electron, X-ray diffraction, and toxicity characteristic leaching processes were performed. When using incineration bottom ash up to 20% of substitution, the compressive strength in all cases was increased. This study showed how the filler effect appeared well in the cement composites through the scanning electron microscopy-backscatter electron, and mercury intrusion porosimetry. X-ray diffraction indicated the possibility of an alkali-silica reaction of the aggregate with the components of incineration bottom ash. This problem is an obstacle to applying the incineration bottom ash as a fine aggregate. In addition, the toxicity characteristic leaching process was shown to be under the threshold of the Korean standard, however, this should nuanced by the consideration of amorphity. Comprehensively, incineration bottom ash could be used as a fine aggregate of up to 20% of substitution. However, the pre-treatment would need to eliminate or reduce alkali reactive components and heavy metals.
Coastal Land-Use and Land-Cover Change Trajectories: Are They Sustainable?
Authors: Faria de Deus Raquel , Tenedório José António
Published: August 2021

In this study, past and current land-use and land-cover (LULC) change trajectories between 1947 and 2018 were analysed in terms of sustainability using a unique set of nine detailed, high-precision LULC thematic maps for the municipality of Portimão (Algarve region), Portugal. Several Geographic Information System (GIS)-based spatial analysis techniques were used to process LULC data and assess the spatiotemporal dynamics of LULC change processes. The dynamics of LULC change were explored by analysing LULC change trajectories. In addition, spatial pattern metrics were introduced to further investigate and quantify the spatial patterns of such LULC change trajectories. The findings show that Portimão has been experiencing complex LULC changes. Nearly 52% of the study area has undergone an LULC change at least once during the 71-year period. The analysis of spatial pattern metrics on LULC change trajectories confirmed the emergence of more complex, dispersed, and fragmented shapes when patches of land were converted from non-built categories into artificial surface categories from 1947 to 2018. The combined analysis of long-term LULC sequences by means of LULC change trajectories and spatial pattern metrics provided useful, actionable, and robust empirical information that can support sustainable spatial planning and smart growth, which is much needed since the results of this study have shown that the pattern of LULC change trajectories in Portimão municipality has been heading towards unsustainability.
Place Attachment in Land Use Changes: A Phenomenological Investigation in Residents’ Lived Experiences with a Renewable Energy Project Deployment
Authors: Mjahed Hammami Samiha , Abdulrahman Al Moosa Heyam
Published: August 2021

Despite growing interest in issues of place attachment and land use changes, scholars of renewable energy have tended to overlook the ways that people–place relations affect local acceptance/opposition of renewable energy projects. We address this gap drawing on the concept of customer experience to capture the meaning of place attachment in a specific context of climate change adaptation (e.g., proposals to site large-scale low-carbon energy technologies such as wind farms) and deepening understanding of the role of place attachment in shaping community responses to the local siting of renewable energy technologies. This research adopts a phenomenological approach that focuses on exploring the residents’ experiences with the local place where they live (a village in Northeast Tunisia) as well as the meanings they attribute to the project. Results show that according to the evaluation of change, whether the renewable energy project enhances or disrupts the different aspects of place experience, residents will exhibit respectively either positive or negative emotions and attitudes and will take action accordingly either by supporting or protesting the project.
Stop Piling on: Assessing Efforts to Reduce Single-Use Water Bottles at Allegheny College
Authors: Bethurem Matthew , Choate Beth , Bramwell Stephanie
Published: August 2021

Plastic waste represents one of the largest environmental problems of the modern era and disposable water bottles comprise a substantial portion of that waste in the U.S. Colleges are in a unique position to create systems that foster sustainable behaviors among their constituents. Since 2015, Allegheny College has installed water refill stations, and initiated a free water bottle program and an awareness campaign to reduce single-use bottles on the college’s campus. Two surveys were distributed to the student body between 2018 and 2019 to assess the efficacy of those initiatives and learn about any barriers. Bottled water use has reduced significantly since 2014, with the majority of students stating that they most commonly drink water from refill stations while on campus. Demographic factors like income, sex, or place of origin were not related to the amount of bottled water consumption or type of water preference among students. The primary barrier to students eliminating disposable water bottle use was found to be a lack of water refill stations in dormitories. Research aimed at changing behaviors to benefit the environment should consider the barrier of convenience. This research provides a valuable lesson that can extend beyond college campuses and into public settings.
Evaluation of Linear Deformation and Unloading Stiffness Characteristics of Asphalt Mixtures Incorporating Various Aggregate Gradations
Authors: Rizvi Mujasim Ali , Khan Ammad Hassan , Rehman Zia ur , Inam Aasim , Masoud Zubair
Published: August 2021

Optimum stiffness and linear deformation in the unloading phase are fundamental properties of asphalt mixtures required for the durability of flexible pavements. In this research, blends of six different aggregate gradations were used for two base course (BC) and four wearing course (WC) asphalt mixtures. Stability and indirect tensile strength of resulting asphalt mixtures were evaluated to relate to viscoelastic unloading deformation and resilient moduli (instantaneous (MRI) and total (MRT)) at 25 °C using a 40/50 binder for 0.1 and 0.3 s load durations. Results indicated that an increase in coarse aggregate proportion from 48 to 70% for BC has shown a 12% and 14% increase in MRT for 0.1 and 0.3 s load durations, respectively, and an increase in coarse aggregate proportion from 41 to 57.5% for WC has caused a 26% and 20% increase in MRI for 0.1 and 0.3 s load durations, respectively. The same coarse aggregate proportions showed an increase in linear viscoelastic deformation at 0.1 s load duration from 54.6 to 68.2% for WC and from 53.0 to 62.7% for BC, whereas for 0.3 s load duration linear viscoelastic deformation increased from 58.1 to 69.1% for WC and 64.3 to 69.2% for BC. The findings of this study will assist in the selection of aggregate gradations to be used in wearing and base course asphalt mixtures for pavement design, construction and maintenance.
Mandatory ESG Reporting and XBRL Taxonomies Combination: ESG Ratings and Income Statement, a Sustainable Value-Added Disclosure
Authors: Faccia Alessio , Manni Francesco , Capitanio Fabian
Published: August 2021

Corporate financial statements address multiple stakeholders’ needs. International Financial Reporting Standards (IFRSs), among others, allow two different classifications, “by function of expense” and “by nature of expense”, for the statement of profit and loss and other comprehensive income for the period (from now on, also identified in short as “Income Statement”, or “IS”). XBRL standards ensure compliance and consistency in financial statements’ drafting and filing. XBRL taxonomies reflect the Income Statement IFRS disclosure requirement in the {310000} and {320000} codifications, respectively. Given the recent EU enhanced regulations that proposed extend mandatory ESG reporting to SMEs, this study aims to design and recommend an additional Income Statement to embed structured Environmental, Social, and Governance (ESG) disclosure. A restatement of the IS is organised following an adjusted Value-Added perspective to fit the purpose of sustainability disclosure. The above-mentioned Income Statement should be suitable and adaptable for entities of any size and operating in any industry. This goal can be achieved through customised input weighting. Therefore, this applied research can fill a current financial ESG disclosure gap, ensuring financial statements’ comparability and encouraging additional mandatory disclosures through standardisation. Two more items in the XBRL (IFRS-based) structure are suggested, leading to the introduction of one fully structured statement “{330000}—Statement of comprehensive income, profit or loss, by Added Value, ESG based” and a semi-structured “{814000}—Notes—ESG Ratings and Reporting” to better discuss and disclose the assumptions and results of the ESG Statement.
Learning from Agricultural Heritage? Lessons of Sustainability from Italian “Coltura Promiscua”
Authors: Ferrario Viviana
Published: August 2021

Agricultural heritage is gaining increasing importance as a repository of lessons to be learned for more sustainable agriculture in the future. Among the forgotten European agricultural heritage, the Italian grapevine “coltura promiscua,” which integrates agroforestry and intercropping, survives only in a few regions in the form of relics. Based on geographic, historic, agricultural literature published on the subject between 16th and 20th century with a focus on North eastern Italy, on previous fieldwork research, and on the analysis of recent candidacies to the Italian National register, this contribution identifies five principles that can be considered today as lessons of sustainability in agriculture: vertical intensification, spatial multifunctionality, resilience through crop diversity, labour-intensive production, personal/familiar/community attachment. Taken together, these principles describe a new rationality that seems to adapt to changed global and local conditions and can suggest new strategies to design new sustainable agricultural systems. The research suggests that sustainability principles can be found both by studying relics of agriculture heritage, and by carefully reading the literature that described them in the past, well before the concept of sustainability itself appeared in the scientific debate. Finally, this paper highlights some difficulties in practicing these lessons in modern agroforestry systems and suggests directions for future research.
Impact of Safety Culture Implementation on Driving Performance among Oil and Gas Tanker Drivers: A Partial Least Squares Structural Equation Modelling (PLS-SEM) Approach
Authors: Al-Mekhlafi Al-Baraa Abdulrahman , Isha Ahmad Shahrul Nizam , Chileshe Nicholas , Abdulrab Mohammed , Kineber Ahmed Farouk , Ajmal Muhammad
Published: August 2021

This research aims to develop a safety culture model by investigating the relationship between safety culture and driving performance. In previous studies, safety culture has been one of the factors that determine safety issues. These issues were then contextually transformed via a pilot study and organized in the form of a theoretical model. The data were collected from 307 oil and gas tanker drivers in Malaysia through questionnaire surveys. Consequently, structural equation models of partial least squares (PLS-SEM) were applied to statistically assess the final model of this study. The results showed that the implementation of safety culture contributes to driving performance at a substantial level; there is a strong association with an effect of 67.3%. The findings of this research would serve as a benchmark for decision-makers in the oil and gas transportation sector, as promoting an awareness of safety culture should boost the efficiency of drivers. This research fills a gap in knowledge by identifying that positive safety culture practices and mindset are direct antecedents for the improvement of driver performance and, thus, the avoidance of road accidents.
Modelling the Impact of Building Information Modelling (BIM) Implementation Drivers and Awareness on Project Lifecycle
Authors: Olanrewaju Oludolapo Ibrahim , Kineber Ahmed Farouk , Chileshe Nicholas , Edwards David John
Published: August 2021

The Architecture, Engineering, Construction and Operations (AECO) industry is generally slow in adopting emerging technologies, and such hesitance invariably restricts performance improvements. A plethora of studies have focused on the barriers, Critical Success Factors (CSFs), lifecycle and drivers independently, but none have explored the impact of BIM drivers and awareness on the project lifecycle. This study empirically explored the impact of BIM drivers and awareness on the project lifecycle using Structural Equation Modelling (SEM). Initially, a conceptual model was developed from an extensive literature review. Thereafter, the model was tested using primary questionnaire data obtained from 90 construction professionals in Lagos, Nigeria. Emergent findings indicate that Building Information Modelling (BIM) drivers have a high impact on BIM awareness at the operation stage of the project lifecycle. The SEM model has an average R2 value of 23% which is moderate. Consequently, this research contributes to the existing body of knowledge by providing invaluable insight into the impact of BIM drivers on BIM awareness in the project lifecycle. Knowledge acquired will help industry stakeholders and government to develop appropriate policies to increase BIM uptake within contemporary practice.
The Integration of Lean and Resilience Paradigms: A Systematic Review Identifying Current and Future Research Directions
Authors: Habibi Rad Mahyar , Mojtahedi Mohammad , Ostwald Michael J.
Published: August 2021

Integration of the lean and resilience paradigms has attracted increasing attention among scientists and practitioners. In an interconnected world, the need to be resilient involves increased readiness to deal with risks from both outside and inside an enterprise, and to be lean involves maximizing value while minimizing waste. The combination of these requirements has been the catalyst for a move towards lean–resilience operations. To better understand this trend, which seeks to help firms retain a competitive position and survive disruptions, this paper provides a systematic literature review of 53 articles identified through the C-I-M-O (context-intervention-mechanism-outcome) framework and examines them using descriptive and content analysis. The results trace the growth of lean–resilience research from its infancy to its current advanced state. This paper also identifies for the first time the lack of structured research on the number and categories of implemented practices and their associated benefits. To address this deficiency, a concept map is developed to provide guidance on the topic, identify gaps and inconsistencies in the literature, understand the state of development and suggest future research directions. The results are used to identify four dominant streams: application, compatibility, integration, and impact assessment in the context of the supply chain, conceptual development and operational research of various organizational and industry sectors. Further topics for investigation are recommended in the form of research questions. The proposed concept map is intended to assist researchers and practitioners to develop knowledge about the integration of lean and resilience paradigms in new contexts and formulate more effective deployment strategies.
The Informal Seed Business: Focus on Yellow Bean in Tanzania
Authors: Sperling Louise , Birachi Eliud , Kalemera Sylvia , Mutua Mercy , Templer Noel , Mukankusi Clare , Radegunda Kessy , William Magdalena , Gallagher Patrick , Kadege Edith , Rubyogo Jean Claude
Published: August 2021

This article explores the informal seed business, focusing on the yellow bean in Tanzania. The yellow bean is a major bean type traded, yet little is known about the seed supply that fuels it. The survey research in 2019 encompassed larger grain traders, informal seed traders, and retailers, covered major production, distribution and sale hubs, and was complemented by GIS mapping of seed and grain flows and DNA fingerprinting of yellow bean samples. Results showed that traders buy and sell grain and informal seed: it is not one business or the other, but both. Informal seed is an important moneymaker, representing between 15 and 40% of trader business in non-sowing and sowing periods, respectively. In the year monitored, 100% of the yellow bean seed was drawn from the informal sector, amounting to $US 4.35 million just among those sampled. Nevertheless, the informal and formal sectors are clearly linked, as over 60% of the beans sampled derived from modern varieties. Informal traders prove key for: sustaining the grain business, serving the core of the seed business, and moving varieties at scale. More explicit efforts are needed to link the informal sector to formal research and development partners in order to achieve even broader impacts.
Sustainable Business Model Innovation: Review, Analysis and Impact on Society
Authors: Molina-Castillo Francisco-Jose , Sinkovics Noemi , Sinkovics Rudolf R.
Published: August 2021

Sustainability issues are on the rise and companies are pressured to respond [...]
COVID-19 Impacts and Sustainability Strategies for Regional Recovery in Southeast Asia: Challenges and Opportunities
Authors: Suriyankietkaew Suparak , Nimsai Suthep
Published: August 2021

The COVID-19 pandemic crisis is threatening our progressive social, ecological and economic development toward achieving the United Nations Sustainable Development Goals (UN SDGs). Studies of its impacts on sustainable development in emerging economies and on fast-growing regional development, such as Southeast Asia or the Association of Southeast Asia Nations (ASEAN), are scarce to date. This paper aims to investigate the COVID-19 impacts and identify challenges and opportunities for possible sustainable recovery solutions with respect to the UN SDGs. We employed a qualitative research method through analytical literature reviews and in-depth interviews with 33 organizations. Our results reveal various pandemic effects, challenges and opportunities for cooperative regional sustainability development and recovery strategies, such as intra-trade strategy, green economy and public–private–people partnerships. The findings provide practical guidance on policy implications for transformative regional sustainability and innovative recovery strategies to achieve the sustainable development agendas (i.e., ASEAN Community Vision 2025 and UN 2030 Agenda). Overall, the paper contributes to advance our limited understanding in this realm and benefits diverse stakeholders toward our sustainable futures.
Enhancement of Cone-Beam Computed Tomography Dental-Maxillofacial Images by Sampling Kantorovich Algorithm
Authors: Costarelli Danilo , Pozzilli Pietro , Seracini Marco , Vinti Gianluca
Published: August 2021

In this paper, we establish a procedure for the enhancement of cone-beam computed tomography (CBCT) dental-maxillofacial images; this can be useful in order to face the problem of rapid prototyping, i.e., to generate a 3D printable file of a dental prosthesis. In the proposed procedure, a crucial role is played by the so-called sampling Kantorovich (SK) algorithm for the reconstruction and image noise reduction. For the latter algorithm, it has already been shown to be effective in the reconstruction and enhancement of real-world images affected by noise in connection to engineering and biomedical problems. The SK algorithm is given by an optimized implementation of the well-known sampling Kantorovich operators and their approximation properties. A comparison between CBTC images processed by the SK algorithm and other well-known methods of digital image processing known in the literature is also given. We finally remark that the above-treated topic has a strong multidisciplinary nature and involves concrete biomedical applications of mathematics. In this type of research, theoretical and experimental disciplines merge in order to find solutions to real-world problems.
Effects of Anodic Aluminum Oxide Substrate Pore Geometry on the Gas-Phase Photocatalytic Activity of ZnO/Al2O3 Composites Prepared by Atomic Layer Deposition
Authors: Stefanov Bozhidar I. , Blagoev Blagoy S. , Österlund Lars , Tzaneva Boriana R. , Angelov George V.
Published: August 2021

We report on the photocatalytic activity of ZnO layers deposited by atomic layer deposition on a porous anodic aluminum oxide substrate with hexagonal pore symmetry and varied pore dimensions. ZnO/Al2O3 composites were prepared with pore diameters in the range 93–134 nm and interpore distance in the range 185–286 nm, and their photocatalytic activity was measured for gas-phase photocatalytic oxidation of acetaldehyde at varying UV illumination intensities (0.08–3.94 mW cm−2). The results show that substrates with narrower pore diameters (<115 nm, in the case of this study) have a detrimental effect on the photocatalyst performance, despite their higher effective surface. The results are explained on the basis of limited mass transfer inside the porous structure and can be used as a guideline in the purposeful design of photocatalysts with a nanoporous or nanotubular structure.
Isolation and Characterisation of the Bundooravirus Genus and Phylogenetic Investigation of the Salasmaviridae Bacteriophages
Authors: Stanton Cassandra R. , Rice Daniel T. F. , Beer Michael , Batinovic Steven , Petrovski Steve
Published: August 2021

Bacillus is a highly diverse genus containing over 200 species that can be problematic in both industrial and medical settings. This is mainly attributed to Bacillus sp. being intrinsically resistant to an array of antimicrobial compounds, hence alternative treatment options are needed. In this study, two bacteriophages, PumA1 and PumA2 were isolated and characterized. Genome nucleotide analysis identified the two phages as novel at the DNA sequence level but contained proteins similar to phi29 and other related phages. Whole genome phylogenetic investigation of 34 phi29-like phages resulted in the formation of seven clusters that aligned with recent ICTV classifications. PumA1 and PumA2 share high genetic mosaicism and form a genus with another phage named WhyPhy, more recently isolated from the United States of America. The three phages within this cluster are the only candidates to infect B. pumilus. Sequence analysis of B. pumilus phage resistant mutants revealed that PumA1 and PumA2 require polymerized and peptidoglycan bound wall teichoic acid (WTA) for their infection. Bacteriophage classification is continuously evolving with the increasing phages’ sequences in public databases. Understanding phage evolution by utilizing a combination of phylogenetic approaches provides invaluable information as phages become legitimate alternatives in both human health and industrial processes.
CARs—A New Perspective to HCMV Treatment
Authors: Bednar Christopher , Ensser Armin
Published: August 2021

Human cytomegalovirus (HCMV), by primary infection or reactivation, represents a great risk for immune-suppressed or compromised patients. In immunocompetent humans, the immune system suppresses the spread of HCMV during an infection, resulting in a mostly asymptomatic or mild course of the disease, whereas in immune suppressed patients, the compromised host immune response cannot control the viral infection. Multiple viral immunomodulatory mechanisms additionally contribute to immune evasion. Use of chimeric antigen receptors (CARs), a treatment strategy adapted from cancer immunotherapy, is investigated for possible application to combat HCMV and other infections in immunocompromised patients. The administration of CAR+ T-cells directed against HCMV antigens can bypass viral immune evasion and may complement existing treatment methods. This review gives a short overview of HCMV, the obstacles of current treatment options as well as a brief introduction to CARs and the current research situation on CAR+ T-cells against HCMV.
Gut Microbiome Changes Associated with Epithelial Barrier Damage and Systemic Inflammation during Antiretroviral Therapy of Chronic SIV Infection
Authors: Tanes Ceylan , Walker Edith M. , Slisarenko Nadia , Gerrets Giovanni L. , Grasperge Brooke F. , Qin Xuebin , Jazwinski S. Michal , Bushman Frederic D. , Bittinger Kyle , Rout Namita
Published: August 2021

Gut dysbiosis is a common feature associated with the chronic inflammation of HIV infection. Toward understanding the interplay of chronic treated HIV infection, dysbiosis, and systemic inflammation, we investigated longitudinal fecal microbiome changes and plasma inflammatory markers in the nonhuman primate model. Following simian immunodeficiency virus (SIV) infection in rhesus macaques, significant changes were observed in several members of the phylum Firmicutes along with an increase in Bacteroidetes. Viral suppression with antiretroviral therapy (ART) resulted in an early but partial recovery of compositional changes and butyrate producing genes in the gut microbiome. Over the course of chronic SIV infection and long-term ART, however, the specific loss of Faecalibacterium prausnitzii and Treponema succinifaciens significantly correlated with an increase in plasma inflammatory cytokines including IL-6, G-CSF, I-TAC, and MIG. Further, the loss of T. succinifaciens correlated with an increase in circulating biomarkers of gut epithelial barrier damage (IFABP) and microbial translocation (LBP and sCD14). As F. prausnitzii and T. succinifaciens are major short-chain fatty acid producing bacteria, their sustained loss during chronic SV-ART may contribute to gut inflammation and metabolic alterations despite effective long-term control of viremia. A better understanding of the correlations between the anti-inflammatory bacterial community and healthy gut barrier functions in the setting of long-term ART may have a major impact on the clinical management of inflammatory comorbidities in HIV-infected individuals.
A Memory of Professor Robert B. Sim, D. Phil
Authors: Yu Chack-Yung
Published: August 2021

Many of us were saddened to learn about the passing of Dr [...]
Acute Late-Stage Myocarditis in the Crab-Eating Macaque Model of Hemorrhagic Smallpox
Authors: Johnson Reed F. , Keith Lauren A. , Cooper Timothy K. , Yellayi Srikanth , Josleyn Nicole M. , Janosko Krisztina B. , Pettitt James D. , Thomasson David , Hagen Katie R. , Gross Robin , Bernbaum John G. , Douglas Debbie , Solomon Jeffrey , Martinez Mark , Cooper Kurt , St. Claire Marisa , Ragland Danny R. , Jahrling Peter B. , Kuhn Jens H. , Arai Andrew E.
Published: August 2021

Hemorrhagic smallpox, caused by variola virus (VARV), was a rare but nearly 100% lethal human disease manifestation. Hemorrhagic smallpox is frequently characterized by secondary bacterial infection, coagulopathy, and myocardial and subendocardial hemorrhages. Previous experiments have demonstrated that intravenous (IV) cowpox virus (CPXV) exposure of macaques mimics human hemorrhagic smallpox. The goal of this experiment was to further understand the onset, nature, and severity of cardiac pathology and how it may contribute to disease. The findings support an acute late-stage myocarditis with lymphohistiocytic infiltrates in the CPXV model of hemorrhagic smallpox.
Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
Authors: Cassaniti Irene , Ferrari Alessandro , Comolli Giuditta , Sarasini Antonella , Gregorini Marilena , Rampino Teresa , Lilleri Daniele , Baldanti Fausto
Published: August 2021

Solid organ transplant recipients, due to the administration of post-transplant immunosuppressive therapies, are at greater risk of viral reactivation episodes, mainly from herpes viruses, including varicella-zoster virus (VZV). The aim of this pilot study was to develop functional immunological assays (VZV-ELISpot) for the quantification and characterization of the VZV-specific effector-memory and central-memory responses in healthy subjects and transplanted patients. Glycoprotein gE and immediate-early 63 (IE-63) were used as antigens for in vitro stimulation. VZV-seropositive healthy subjects showed higher responses in respect to seronegative subjects. Even if differences were observed between VZV-seropositive healthy subjects and transplanted subjects at pre-transplant, the VZV-specific T-cell response was reduced at 60 days after transplant, mainly for the high level of immunosuppression. Phenotypical characterization revealed that response against VZV was mainly mediated by CD4 T cells. The results obtained in this study might be useful for the definition of personalized follow-up of the transplanted patients, providing useful information on the status of the patient potentially at risk of viral reactivation or other opportunistic infections.
Comparison of Detecting and Quantitating SARS-CoV-2 in Wastewater Using Moderate-Speed Centrifuged Solids versus an Ultrafiltration Method
Authors: Hasing Maria , Yu Jiaao , Qiu Yuanyuan , Maal-Bared Rasha , Bhavanam Sudha , Lee Bonita , Hrudey Steve , Pang Xiaoli
Published: August 2021

Mounting evidence suggests that solids are a reliable matrix for SARS-CoV-2 detection in wastewater, yet studies comparing solids-based methods and common concentration methods using the liquid fraction remain limited. In this study, we developed and optimized a method for SARS-CoV-2 detection in wastewater using moderate-speed centrifuged solids and evaluated it against an ultrafiltration reference method. SARS-CoV-2 was quantified in samples from 12 wastewater treatment plants from Alberta, Canada, using RT-qPCR targeting the N2 and E genes. PCR inhibition was examined by spiking salmon DNA. The effects of using different amounts of solids, adjusting the sample pH to 9.6–10, and modifying the elution volume at the final step of RNA extraction were evaluated. SARS-CoV-2 detection rate in solids from 20 mL of wastewater showed no statistically significant difference compared to the ultrafiltration method (97/139 versus 90/139, p = 0.26, McNemar’s mid-p test). The optimized wastewater solids-based method had a significantly lower rate of samples with PCR inhibition versus ultrafiltration (3% versus 9.5%, p = 0.014, Chi-square test). Our optimized moderate-speed centrifuged solids-based method had similar sensitivity when compared to the ultrafiltration reference method but had the added advantages of lower costs, fewer processing steps, and a shorter turnaround time.
Numerical Modeling of Saltwater Intrusion in the Rmel-Oulad Ogbane Coastal Aquifer (Larache, Morocco) in the Climate Change and Sea-Level Rise Context (2040)
Authors: EL Hamidi Mohamed Jalal , Larabi Abdelkader , Faouzi Mohamed
Published: August 2021

Many coastal aquifers have experienced seawater intrusion (SWI) into fresh groundwater aquifers. The principal causes of SWI include over-pumping and events such as climate change (CC) and rising sea levels. In northern Morocco, the Rmel-Oulad Ogbane coastal aquifer (ROOCA) supplies high-quality groundwater for drinking water and agriculture. This favorable situation has led to increased pumping, resulting in environmental challenges such as dropping water table and SWI. Furthermore, the climate has resulted in less recharge, with an estimated annual precipitation of 602 mm and an average temperature of 18.5 °C. The goal of this study is to determine how CC, over-pumping, and sea-level rise (SLR) affect SWI. Computational groundwater and solute transport models are used to simulate the spatial and temporal evolution of hydraulic heads and groundwater solute concentrations. The calibration is based on steady and transient groundwater levels from 1962 to 2040. SWI simulations show that the NW sector of the coastal area would be polluted, with the toe reaching 5.2 km inland with a significant salinity (15–25 g/L). To protect the fresh water in the reservoir from SWI, enhanced groundwater development and management approaches for this aquifer are required, such as artificial recharge from surface water.
Variations and Drivers of Methane Fluxes from Double-Cropping Paddy Fields in Southern China at Diurnal, Seasonal and Inter-Seasonal Timescales
Authors: Ma Linhua , Liu Bo , Cui Yuanlai , Shi Yuanzhi
Published: August 2021

Methane (CH4) is the second most important anthropogenic greenhouse gas (GHG) after carbon dioxide (CO2), and paddy fields are among the largest sources of CH4 emissions. Owing to the scarcity of observational data, the characteristics and influencing factors of CH4 fluxes in paddy fields at different timescales need to be further investigated. Observations of CH4 fluxes via eddy covariance (EC) data were performed over four seasons in two paddy fields in Nanchang, Jiangxi Province, China. The pattern, magnitude and biophysical controls of CH4 emissions were explored by wavelet analysis and stepwise multiplicative modelling. The results revealed a distinct, single-peak diurnal pattern in CH4 fluxes during the vegetative stage in all four rice growing seasons and the reproductive stage of early rice. Large seasonal variations in daily CH4 emissions were observed in the two double-cropping paddy fields, and the soil temperature at a depth of 5 cm (Ts5) explained most of the seasonality of the CH4 fluxes. At the inter-seasonal scale, under local farms’ traditional field management method, reducing the amount and frequency of irrigation during the vegetative stage could decrease CH4 emissions in southern China. This study improves the understanding of CH4 emissions and helps in developing GHG management strategies for paddy fields.
Seasonal Variations in the Biochemical Compositions of Phytoplankton and Transparent Exopolymer Particles (TEPs) at Jang Bogo Station (Terra Nova Bay, Ross Sea), 2017–2018
Authors: Park Sanghoon , Park Jisoo , Yoo Kyu-Cheul , Yoo Jaeill , Kim Kwanwoo , Jo Naeun , Jang Hyo-Keun , Kim Jaehong , Kim Jaesoon , Kim Joonmin , Lee Sang-Heon
Published: August 2021

The biochemical composition of particulate organic matter (POM) mainly originates from phytoplankton. Transparent exopolymer particles (TEPs) depend on environmental conditions and play a role in the food web and biogeochemical cycle in marine ecosystems. However, little information on their characteristics in the Southern Ocean is available, particularly in winter. To investigate the seasonal characteristics of POM and TEPSs, seawater samples were collected once every two weeks from November 2017 to October 2018 at Jang Bogo Station (JBS) located on the coast of Terra Nova Bay in the Ross Sea. The total chlorophyll-a (Chl-a) concentrations increased from spring (0.08 ± 0.06 μg L−1) to summer (0.97 ± 0.95 μg L−1) with a highest Chl-a value of 2.15 μg L−1. After sea ice formation, Chl-a rapidly decreased in autumn (0.12 ± 0.10 μg L−1) and winter (0.01 ± 0.01 μg L−1). The low phytoplankton Chl-a measured in this study was related to a short ice-free period in summer. Strong seasonal variations were detected in the concentrations of proteins and lipids (one-way ANOVA test, p < 0.05), whereas no significant difference in carbohydrate concentrations was observed among different seasons (one-way ANOVA test, p > 0.05). The phytoplankton community was mostly composed of diatoms (88.8% ± 11.6%) with a large accumulation of lipids. During the summer, the POM primarily consisted of proteins. The composition being high in lipids and proteins and the high caloric content in summer indicated that the phytoplankton would make a good food source. In winter, the concentrations of proteins decreased sharply. In contrast, relatively stable concentrations of carbohydrates and lipids have been utilized for respiration and long-term energy storage in the survival of phytoplankton. The TEPS values were significantly correlated with variations in the biomass and species of the phytoplankton. Our study site was characterized by dominant diatoms and low Chl-a concentrations, which could have resulted in relatively low TEP concentrations compared to other areas. The average contributions of TEP-C to the total POC were relatively high in autumn (26.9% ± 6.1%), followed by those in summer (21.9% ± 7.1%), winter (13.0% ± 4.2%), and spring (9.8% ± 3.1%).