bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel 13 bp Deletion within the NR6A1 Gene Is Significantly Associated with Growth Traits in Donkeys
Authors: Fang Xiya , Lai Zhenyu , Liu Jie , Zhang Chunlan , Li Shipeng , Wu Fei , Zhou Zihui , Lei Chuzhao , Dang Ruihua
Published: September 2019

Nuclear receptor subfamily 6, group A, member 1 (NR6A1), as an important member of the nuclear receptor family, plays an important role in regulating growth, metabolism, and differentiation of embryonic stem cells. For this reason, the NR6A1 gene is considered to be a promising candidate for economic traits and was found to be associated with body size traits in many livestock. However, no studies have been conducted on NR6A1 in donkeys so far. Thus, in this research, we focused on donkeys and identified a 13 bp deletion in intron-1 of the NR6A1 gene among 408 individuals from Guanzhong and Dezhou donkeys using polyacrylamide gel electrophoresis. Three genotypes were identified, namely II, ID, and DD. The association analysis indicated that the body lengths and body heights5f genotype II individuals were significantly different to those of genotype ID in Dezhou donkeys. Conclusively, the 13 bp deletion was associated with growth traits in both Guanzhong donkeys and Dezhou donkeys, indicating that the NR6A1 gene could be a possible candidate gene in marker-assisted selection for donkey breeding programs.
Effects of Partial Replacement of Conventional with Alternative Feeds on Nutrient Intake, Digestibility, Milk Yield and Composition of Awassi Ewes and Lambs
Authors: Aloueedat Mohammad K. , Obeidat Belal S. , Awawdeh Mofleh S.
Published: September 2019

Two experiments were done to assess the effects of alternative feeds (AF; dried distillers grains with solubles, carob pods, olive cake, and bread by-product) on lactating performance of ewes and digestibility and nitrogen (N) retention of lambs. Diets were: no AF (CON); 200 g/kg AF (AF200); and 400 g/kg AF (AF400). In Experiment 1, 27 Awassi ewes were randomly distributed into three groups, and each was fed one of the diets described before (9 ewes/diet). Evaluation of milk composition and yield was performed at the beginning of the experiment and on days 18, 36, and 54. In Experiment 2, 18 Awassi lambs were allocated to the same diets (6 ewe lambs/diet) during a 21-day trial (14 days housed individually in shaded pens and 7 days in metabolic cages). In Experiment 1, no differences in body weight (BW) of the ewes and their lambs were detected. With the exception of neutral detergent fiber intake, which was lower in the AF-containing diets compared with CON, dry matter, crude protein, and acid detergent fiber intake were not affected by dietary treatment. Milk yield and composition was comparable among diets, and the cost of milk production was lower in AF compared to CON diet. In Experiment 2, nutrient digestibility and N retention were not affected by the diet. Results showed the possibility of including different AF in ewe and lamb diets to mitigate production cost without negatively affecting intake, milk yield, and composition, digestibility, and animal welfare and health.
Lentivirus Susceptibility in Iranian and German Sheep Assessed by Determination of TMEM154 E35K
Authors: Molaee Vahid , Otarod Vahid , Abdollahi Darab , Lühken Gesine
Published: September 2019

Small ruminant lentiviruses (SRLVs) cause maedi-visna disease in sheep and are prevalent in Iran and Germany. The association of the transmembrane protein 154 (TMEM154) variants with SRLV infection has been previously identified by a genome-wide association (GWAS) approach and subsequent analyses, and validated in some US, German, and Turkish sheep flocks. We aimed at evaluating these findings for the first time in Iranian, and in some more German sheep flocks/breeds. Also, we aimed at comparing the SRLV susceptibility in Iranian and German sheep based on the frequency of the TMEM154 E35 allele. About 800 blood samples were collected from 21 Iranian and German sheep flocks/breeds for different purposes: (1) The association of TMEM154 E35K with SRLV infection status was tested in four sheep breeds and found to be significant in Kermani, Merinoland, and Brown Hair. (2) The usefulness of the TMEM154 E35 frequency for predicting SRLV susceptibility was evaluated by regression analysis, combining data from this study and some already published data. Results showed a significant association between E35 frequency and SRLV prevalence. (3) SRLV susceptibility was compared based on E35 frequency in Iranian and German sheep. Altogether, findings of this study provide valuable information on SRLV susceptibility, using TMEM154 E35, in Iranian and German sheep.
2-Methoxy-7-Acetonyljuglone Isolated from Reynoutria japonica Increases the Activity of Nuclear Factor Erythroid 2-Related Factor-2 through Inhibition of Ubiquitin Degradation in HeLa Cells
Authors: Kim Jung-Hwan , Khalil Atif Ali Khan , Kim Hye-Jin , Kim Sung-Eun , Ahn Mi-Jeong
Published: September 2019

The nuclear factor erythroid-derived 2-related factor 2 (NRF2) is a key transcription factor for the activation of genes responsible for oxidative stress and drug detoxification. Thus, it is important to identify NRF2 activators, which can be used to protect the cells from oxidative damage. Here, we investigated the effect of juglone derivatives isolated from Reynoutria japonica on the activity of NRF2 in HeLa cells. We demonstrated that among the juglone derivatives, 2-methoxy-7-acetonyljuglone (MA) strongly stimulated the antioxidant response element (ARE)-luciferase activity in a dose-dependent manner. In addition, MA significantly increased the nuclear localization of NRF2 and, consequently, increased the expression of NRF2 target genes, including heme oxygenase-1(HO-1), NAD(P)H: quinine oxidoreductase-1 (NQO-1), and glutamate-cysteine ligase catalytic (GCLC). To gain insights into the NRF2 signaling mechanism by MA, we measured the activities of RAC-alpha serine/threonine-protein kinase (AKT) and mitogen-activated protein (MAP) kinase family proteins, including extracellular signal-regulated kinase (ERK) and p38. Our results showed that MA induced NRF2 activity through p38 and AKT signaling. Subsequently, we found that MA significantly enhanced NRF2 stability by inhibiting ubiquitin-dependent proteasomal degradation. Thus, MA might protect cells by enhancing the activity and stability of NRF2 through inhibition of the proteasomal degradation pathway.
Plasma and Aerosols: Challenges, Opportunities and Perspectives
Authors: Stancampiano Augusto , Gallingani Tommaso , Gherardi Matteo , Machala Zdenko , Maguire Paul , Colombo Vittorio , Pouvesle Jean-Michel , Robert Eric
Published: September 2019

The interaction of plasmas and liquid aerosols offers special advantages and opens new perspectives for plasma–liquid applications. The paper focuses on the key research challenges and potential of plasma-aerosol interaction at atmospheric pressure in several fields, outlining opportunities and benefits in terms of process tuning and throughputs. After a short overview of the recent achievements in plasma–liquid field, the possible application benefits from aerosol injection in combination with plasma discharge are listed and discussed. Since the nature of the chemicophysical plasma-droplet interactions is still unclear, a multidisciplinary approach is recommended to overcome the current lack of knowledge and to open the plasma communities to scientists from other fields, already active in biphasic systems diagnostic. In this perspective, a better understanding of the high chemical reactivity of gas–liquid reactions will bring new opportunities for plasma assisted in-situ and on-demand reactive species production and material processing.
Experimental Measurements of the Natural Frequencies and Mode Shapes of Rotating Disk-Blades-Disk Assemblies from the Stationary Frame
Authors: Presas Alexandre , Valentin David , Valero Carme , Egusquiza Monica , Egusquiza Eduard
Published: September 2019

Determining the natural frequencies and mode shapes of rotating turbomachinery components from both rotating and stationary reference frames is of paramount importance to avoid resonance problems that could affect the normal operation of the machine, or even cause critical damages in these components. Due to their similarity to real engineering cases, this topic has been experimentally analyzed in the past for disk-shaft assemblies and rotor disk-blades assemblies (bladed-disk or blisk). The same topic is less analyzed for disk-blades-disk assemblies, although such configurations are widely used in centrifugal closed impellers of compressors, hydraulic pumps, pump-turbines, and runners of high head Francis turbines. In this paper, experimental measurements, varying the rotating speed of a disk-blade-disk assembly and exciting the first natural frequencies of the rotating frame, have been performed. The rotating structure is excited and measured by means of PZT patches from the rotating frame and with a Laser Doppler Vibrometer (LDV). In order to interpret the experimental results obtained from the stationary frame, a method to decompose the diametrical mode shapes of the structure in simple diametrical components (which define the diametrical mode shapes of a simple disk) has been proposed. It is concluded that the resonant frequencies detected with a stationary sensor correspond to the ones predicted with the decomposition method. Finally, a means to obtain equivalent results with numerical simulation methods is shown.
The Potential of Additive Manufacturing in the Smart Factory Industrial 4.0: A Review
Authors: Mehrpouya Mehrshad , Dehghanghadikolaei Amir , Fotovvati Behzad , Vosooghnia Alireza , Emamian Sattar S. , Gisario Annamaria
Published: September 2019

Additive manufacturing (AM) or three-dimensional (3D) printing has introduced a novel production method in design, manufacturing, and distribution to end-users. This technology has provided great freedom in design for creating complex components, highly customizable products, and efficient waste minimization. The last industrial revolution, namely industry 4.0, employs the integration of smart manufacturing systems and developed information technologies. Accordingly, AM plays a principal role in industry 4.0 thanks to numerous benefits, such as time and material saving, rapid prototyping, high efficiency, and decentralized production methods. This review paper is to organize a comprehensive study on AM technology and present the latest achievements and industrial applications. Besides that, this paper investigates the sustainability dimensions of the AM process and the added values in economic, social, and environment sections. Finally, the paper concludes by pointing out the future trend of AM in technology, applications, and materials aspects that have the potential to come up with new ideas for the future of AM explorations.
Experimental Investigation on Semi-Active Control of Base Isolation System Using Magnetorheological Dampers for Concrete Frame Structure
Authors: Fu Weiqing , Zhang Chunwei , Li Mao , Duan Cunkun
Published: September 2019

The traditional passive base isolation is the most widely used method in the engineering practice for structural control, however, it has the shortcoming that the optimal control frequency band is significantly limited and narrow. For the seismic isolation system designed specifically for large earthquakes, the structural acceleration response may be enlarged under small earthquakes. If the design requirements under small earthquakes are satisfied, the deformation in the isolation layer may become too large to be accepted. Occasionally, it may be destroyed under large earthquakes. In the isolation control system combined with rubber bearing and magnetorheological (MR) damper, the MR damper can provide instantaneous variable damping force to effectively control the structural response at different input magnitudes. In this paper, the control effect of semi-active control and quasi-passive control for the isolation control system is verified by the shaking table test. In regard to semi-active control, the linear quadratic regulator (LQR) classical linear optimal control algorithm by continuous control and switch control strategies are used to control the structural vibration response. Numerical simulation analysis and shaking table test results indicate that isolation control system can effectively overcome the shortcoming due to narrow optimum control band of the passive isolation system, and thus to provide optimal control for different seismic excitations in a wider frequency range. It shows that, even under super large earthquakes, the structure still exhibits the ability to maintain overall stability performance.
Determining the Variability of the Territorial Sea Baseline on the Example of Waterbody Adjacent to the Municipal Beach in Gdynia
Authors: Specht Mariusz , Specht Cezary , Wąż Mariusz , Dąbrowski Paweł , Skóra Marcin , Marchel Łukasz
Published: September 2019

The purpose of this publication is to analyze the spatial and temporal variability of the territorial sea baseline in sand bottom waterbodies, which were determined twice, in 2016 and 2018, by the Real Time Kinematic (RTK) method. This involves direct measurement of sea bottom coordinates on planned hydrographic sounding profiles using a Global Navigation Satellite System (GNSS) receiver mounted on a pole. The data were the basis for creating Digital Terrain Models (DTM), which were then used to determine the baseline for both measurement campaigns. Subsequently, terrain surface models were compared to determine bathymetry changes in the area under analysis, and an assessment was made of the baseline spatial position change over the previous two years. The measurements have shown considerable spatial and temporal variability of the baseline course along a short section of sandy beach. The territorial sea baseline was very unstable; in some places, it moved by even 20–25 m, landwards and seawards. Therefore, one can suppose that these changes are periodic, and one can conclude that the reliability of the baseline measurements can decrease quite quickly.
A G-Code Generator for Volumetric Models
Authors: Ueng Shyh-Kuang , Huang Hsuan-Kai , Huang Hsin-Cheng
Published: September 2019

In layered manufacturing (LM), slicers are employed to convert input geometric models into G-codes. Conventional slicers accept only surface models as input data. Thus, volumetric models have to be converted into polygonal representations to fit the data format of the slicers. This results in extra computational costs and geometric errors. In this article, we present an efficient slicer aiming to generate G-codes for volumetric models. At first, our slicer computes the printing direction by exploring the inertia tensor of the input model to enhance the stability of the printed part and to decrease the build time. Then, it detects and classifies overhangs in the input model and generates necessary support structures by using a pattern-based method. Thirdly, the proposed slicer divides the input model into the skin and internal regions and cuts the model into 2D images. Subsequently, these images are transformed into toolpaths by utilizing texture mapping and graph traversal methods. Finally, the resultant toolpaths are smoothed to reduce staircases and encoded into G-codes. Test results verify that the proposed slicer produces decent G-codes for volumetric models. Scanned objects hidden in volume data can be directly manufactured without generating intermediate polygonal representations. LM processes become more efficient.
Applied Sciences Special Issue: Ultrasonic Guided Waves
Authors: Lissenden Clifford J.
Published: September 2019

The propagation of ultrasonic guided waves in solids is an important area of scientific inquiry due primarily to their practical applications for the nondestructive characterization of materials, such as nondestructive inspection, quality assurance testing, structural health monitoring, and for achieving material state awareness [...]
Synthetic Aperture Radar Interferometry (InSAR) Ionospheric Correction Based on Faraday Rotation: Two Case Studies
Authors: Zhu Wu , Jung Hyung-Sup , Chen Jing-Yuan
Published: September 2019

Spaceborne synthetic aperture radar (SAR) imagery is affected by the ionosphere, resulting in distortions of the SAR intensity, phase, and polarization. Although several methods have been proposed to mitigate the ionospheric phase delay of SAR interferometry, the application of them with full-polarimetric SAR interferometry is limited. Based on this background, Faraday rotation (FR)-based methods are used in this study to mitigate the ionospheric phase errors on full-polarimetric SAR interferometry. For a performance test of the selected method, L-band Advanced Land Observation Satellite (ALOS) Phase Array L-band SAR (PALSAR) full-polarimetric SAR images over high-latitude and low-latitude regions are processed. The result shows that most long-wavelength ionospheric phase errors are removed from the original phase after using the FR-based method, where standard deviations of the corrected result have decreased by almost a factor of eight times for the high-latitude region and 28 times for low-latitude region, compared to those of the original phase, demonstrating the efficiency of the method. This result proves that the FR-based method not only can mitigate the ionospheric effect on SAR interferometry, but also can map the high-spatial-resolution vertical total electronic content (VTEC) distribution.
Nonlinear Vibration Analysis of a Beam with a Breathing Crack
Authors: Long Hui , Liu Yilun , Liu Kefu
Published: September 2019

The phenomena of sub- and super-harmonic responses make up one of the prominent nonlinear characteristics of a beam with a breathing crack. In order to fully understand the behaviors of sub- and super-harmonic resonances, it is necessary to analyze the nonlinear vibration of a beam-like structure with a breathing crack. In this study, a new stiffness model that considers the influence of the partial crack closure is proposed to model the stiffness variation of the cracked beam. Based on the finite element model of a beam with a breathing crack, the multiple-scale method is proposed to analyze the nonlinear vibration of a cracked beam subjected to harmonic excitation, and the relation between the nonlinear vibration of the cracked beam and the system parameters is obtained. An experiment is conducted to validate the analytical results. The study shows that the nonlinear responses of a beam with a breathing crack are affected by both the structural parameters and the crack parameters.
A Neuronal Morphology Classification Approach Based on Locally Cumulative Connected Deep Neural Networks
Authors: Lin Xianghong , Zheng Jianyang
Published: September 2019

Neurons are the basic building and computational units of the nervous system, and have complex and diverse spatial geometric structures. By solving the neuronal classification problem, we can further understand the characteristics of neurons and the process of information transmission. This paper presents a neuronal morphology classification approach based on locally cumulative connected deep neural networks, where 43 geometric features were extracted from two different neuron datasets and applied to classify types of neurons. Then, the effects of different parameters of deep learning networks on the performance of neuron classification were analyzed including mini-batch size, number of intermediate layers, and number of building blocks. The accuracy of the approach was also compared with that of the other mainstream machine learning approaches. The experimental results showed that the proposed approach is effective for solving complex neuronal morphology classification problems.
Frontal Wind Field Retrieval Based on UHF Wind Profiler Radars and S-Band Radars Network
Authors: Kim Min-Seong , Campistron Bernard , Kwon Byung Hyuk
Published: September 2019

The three-dimensional wind field (WPR3D) and the multiple WPR3D (M-WPR3D) associated with the passage of a stationary front was derived from observations made by a network of eight wind profiler radars (WPR) being operated by the Korea Meteorological Administration during the summer “Jangma” season. The effectiveness of the WPR3D was determined through numerical model analysis and wind profilers at three sites, and the accuracy of the M-WPR3D was validated by comparing the trajectory of the radiosonde. The discontinuity of the wind field near the frontal interface was clearly retrieved and the penetration of the air mass in the southern front was detected. Compared with either the wind vector of three single wind profiler or a local data assimilation and predication system, the WPR3D wind field showed a wind speed accuracy of approximately 70% at an altitude of 1.5 km and underestimated the wind speed by 0.5–1.5 m s−1. The M-WPR3D with three S-band Doppler radars successfully retrieved the backing wind field as well as the pre-Jangma-frontal jet. The results of this study showed that severe weather can be effectively analyzed using a three-dimensional wind field generated on the basis of a remote sensing network.
Validation and Accuracy Assessment of MODIS C6.1 Aerosol Products over the Heavy Aerosol Loading Area
Authors: Tian Xinpeng , Gao Zhiqiang
Published: September 2019

The aim of this study is to evaluate the accuracy of MODerate resolution Imaging Spectroradiometer (MODIS) aerosol optical depth (AOD) products over heavy aerosol loading areas. For this analysis, the Terra-MODIS Collection 6.1 (C6.1) Dark Target (DT), Deep Blue (DB) and the combined DT/DB AOD products for the years 2000–2016 are used. These products are validated using AErosol RObotic NETwork (AERONET) data from twenty-three ground sites situated in high aerosol loading areas and with available measurements at least 500 days. The results show that the numbers of collections (N) of DB and DT/DB retrievals were much higher than that of DT, which was mainly caused by unavailable retrieval of DT in bright reflecting surface and heavy pollution conditions. The percentage falling within the expected error (PWE) of the DT retrievals (45.6%) is lower than that for the DB (53.4%) and DT/DB (53.1%) retrievals. The DB retrievals have 5.3% less average overestimation, and 25.7% higher match ratio than DT/DB retrievals. It is found that the current merged aerosol algorithm will miss some cases if it is determined only on the basis of normalized difference vegetation index. As the AOD increases, the value of PWE of the three products decreases significantly; the undervaluation is suppressed, and the overestimation is aggravated. The retrieval accuracy shows distinct seasonality: the PWE is largest in autumn or winter, and smallest in summer. The most severe overestimation and underestimation occurred in the summer. Moreover, the DT, DB and DT/DB products over different land cover types still exhibit obvious deviations. In urban areas, the PWE of DB product (52.6%) is higher than for the DT/DB (46.3%) and DT (25.2%) products. The DT retrievals perform poorly over the barren or sparsely vegetated area (N = 52). However, the performance of three products is similar over vegetated area. On the whole, the DB product performs better than the DT product over the heavy aerosol loading area.
Parasitism of the Invasive Brown Marmorated Stink Bug, Halyomorpha halys (Hemiptera: Pentatomidae), by the Native Parasitoid, Trichopoda pennipes (Diptera: Tachinidae)
Authors: Joshi Neelendra K. , Leslie Timothy W. , Biddinger David J.
Published: September 2019

The invasive brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), has been an important agricultural pest in the Mid-Atlantic United States since its introduction in 1996. Biological control by native species may play an important role in suppressing H. halys populations and reduce reliance on chemical control. We collected H. halys adults in agricultural areas of five Pennsylvania counties over two years to examine the extent and characteristics of adult stink bug parasitism by Trichopoda pennipes (Diptera: Tachinidae), a native parasitoid of hemipterans. The overall parasitism rate (in terms of T. pennipes egg deposition) was 2.38 percent. Rates differed among counties and seasons, but not between years. Instances of supernumerary oviposition were evident, and eggs were more commonly found on the ventral side of the thorax, although no differences in egg deposition were found between males and female hosts. T. pennipes has begun to target H. halys adults in Pennsylvania and has the potential to play a role in regulating this pest. Adult parasitism of H. halys by T. pennipes should continue to be monitored, and landscape management and ecological pest management practices that conserve T. pennipes populations should be supported in agricultural areas where H. halys is found.
Origin of Carbon and Essential Fatty Acids in Higher Trophic Level Fish in Headwater Stream Food Webs
Authors: Fujibayashi Megumu , Miura Yoshie , Suganuma Reina , Takahashi Shinji , Sakamaki Takashi , Miyata Naoyuki , Kazama So
Published: September 2019

Dietary carbon sources in headwater stream food webs are divided into allochthonous and autochthonous organic matters. We hypothesized that: 1) the dietary allochthonous contribution for fish in headwater stream food webs positively relate with canopy cover; and 2) essential fatty acids originate from autochthonous organic matter regardless of canopy covers, because essential fatty acids, such as 20:5ω3 and 22:6ω3, are normally absent in allochthonous organic matters. We investigated predatory fish Salvelinus leucomaenis stomach contents in four headwater stream systems, which are located in subarctic region in northern Japan. In addition, stable carbon and nitrogen isotope ratios, fatty acid profile, and stable carbon isotope ratios of essential fatty acids were analyzed. Bulk stable carbon analysis showed the major contribution of autochthonous sources to assimilated carbon in S. leucomaenis. Surface baits in the stomach had intermediate stable carbon isotope ratios between autochthonous and allochthonous organic matter, indicating aquatic carbon was partly assimilated by surface baits. Stable carbon isotope ratios of essential fatty acids showed a positive relationship between autochthonous sources and S. leucomaenis across four study sites. This study demonstrated that the main supplier of dietary carbon and essential fatty acids was autochthonous organic matter even in headwater stream ecosystems under high canopy cover.
HDX and Native Mass Spectrometry Reveals the Different Structural Basis for Interaction of the Staphylococcal Pathogenicity Island Repressor Stl with Dimeric and Trimeric Phage dUTPases
Authors: Nyíri Kinga , Harris Matthew J. , Matejka Judit , Ozohanics Olivér , Vékey Károly , Borysik Antoni J. , Vértessy Beáta G.
Published: September 2019

The dUTPase enzyme family plays an essential role in maintaining the genome integrity and are represented by two distinct classes of proteins; the β-pleated homotrimeric and the all-α homodimeric dUTPases. Representatives of both trimeric and dimeric dUTPases are encoded by Staphylococcus aureus phage genomes and have been shown to interact with the Stl repressor protein of S. aureus pathogenicity island SaPIbov1. In the present work we set out to characterize the interactions between these proteins based on a range of biochemical and biophysical methods and shed light on the binding mechanism of the dimeric φNM1 phage dUTPase and Stl. Using hydrogen deuterium exchange mass spectrometry, we also characterize the protein regions involved in the dUTPase:Stl interactions. Based on these results we provide reasonable explanation for the enzyme inhibitory effect of Stl observed in both types of complexes. Our experiments reveal that Stl employs different peptide segments and stoichiometry for the two different phage dUTPases which allows us to propose a functional plasticity of Stl. The malleable character of Stl serves as a basis for the inhibition of both dimeric and trimeric dUTPases.
Timing-Dependent Protection of Swimming Exercise against d-Galactose-Induced Aging-Like Impairments in Spatial Learning/Memory in Rats
Authors: Li Xue , Wang Lu , Zhang Shuling , Hu Xiang , Yang Huijun , Xi Lei
Published: September 2019

This study was designed to investigate beneficial effects of swimming exercise training on learning/memory, synaptic plasticity and CREB (cAMP response element binding protein) expression in hippocampus in a rat model of d-galactose-induced aging (DGA). Eighty adult male rats were randomly divided into four groups: Saline Control (group C), DGA (group A), Swimming exercise before DGA (group S1), and Swimming during DGA (group S2). These four groups of animals were further divided into Morris water maze training group (M subgroup) and sedentary control group (N subgroup). Spatial learning/memory was tested using Morris water maze training. The number and density of synaptophysin (Syp) and metabotropic glutamate receptor 1 (mGluR1) in hippocampal dentate gyrus area, CREB mRNA and protein expression and DNA methylation levels were determined respectively with immunohistochemistry, western blot, real-time PCR, and MassArray methylation detection platform. We found that compared with group C, DGA rats showed aging-like poor health and weight loss as well as hippocampal neurodegenerative characteristics. Exercise training led to a time-dependent decrease in average escape latency and improved spatial memory. Exercise training group (S2M) had significantly increased swim distance as compared with controls. These functional improvements in S2M group were associated with higher Syp and mGluR1 values in hippocampus (p < 0.01) as well as higher levels of hippocampal CREB protein/mRNA expression and gene methylation. In conclusion, swimming exercise training selectively during drug-induced aging process protected hippocampal neurons against DGA-elicited degenerative changes and in turn maintained neuronal synaptic plasticity and learning/memory function, possibly through upregulation of hippocampal CREB protein/mRNA and reduction of DGA-induced methylation of CREB.
Green Roofs and Greenpass
Authors: Scharf Bernhard , Kraus Florian
Published: September 2019

The United Nations have identified climate change as the greatest threat to human life. As current research shows, urban areas are more vulnerable to climate change than rural areas. Numerous people are affected by climate change in their daily life, health and well-being. The need to react is undisputed and has led to numerous guidelines and directives for urban climate adaptation. Plants are commonly mentioned and recommended as one key to urban climate adaptation. Due to shading of open space and building surfaces, as well as evapotranspiration, plants reduce the energy load on the urban fabric and increase thermal comfort and climate resilience amongst many other ecosystem services. Plants, therefore, are described as green infrastructure (GI), because of the beneficial effects they provide. Extensive green roofs are often discussed regarding their impact on thermal comfort for pedestrians and physical properties of buildings. By means of Stadslab2050 project Elief Playhouse in Antwerp, Belgium, a single-story building in the courtyard of a perimeter block, the effects of different extensive green roof designs (A and B) on the microclimate, human comfort at ground and roof level, as well as building physics are analyzed and compared to the actual roofing (bitumen membrane) as the Status Quo variant. For the analyses and evaluation of the different designs the innovative Green Performance Assessment System (GREENPASS®) method has been chosen. The planning tool combines spatial and volumetric analyses with complex 3D microclimate simulations to calculate key performance indicators such as thermal comfort score, thermal storage score, thermal load score, run-off and carbon sequestration. Complementary maps and graphs are compiled. Overall, the chosen method allows to understand, compare and optimize project designs and performance. The results for the Elief Playhouse show that the implementation of green roofs serves a slight contribution to the urban energy balance but a huge impact on the building and humans. Variant B with entire greening performs better in all considered indicators, than the less greened design Variant A and the actual Status Quo. Variant B will probably bring a greater cost/benefit than Variant A and is thus recommended.
Hyperspectral Imaging of Head and Neck Squamous Cell Carcinoma for Cancer Margin Detection in Surgical Specimens from 102 Patients Using Deep Learning
Authors: Halicek Martin , Dormer James D. , Little James V. , Chen Amy Y. , Myers Larry , Sumer Baran D. , Fei Baowei
Published: September 2019

Surgical resection of head and neck (H and N) squamous cell carcinoma (SCC) may yield inadequate surgical cancer margins in 10 to 20% of cases. This study investigates the performance of label-free, reflectance-based hyperspectral imaging (HSI) and autofluorescence imaging for SCC detection at the cancer margin in excised tissue specimens from 102 patients and uses fluorescent dyes for comparison. Fresh surgical specimens (n = 293) were collected during H and N SCC resections (n = 102). The tissue specimens were imaged with reflectance-based HSI and autofluorescence imaging and afterwards with two fluorescent dyes for comparison. A histopathological ground truth was made. Deep learning tools were developed to detect SCC with new patient samples (inter-patient) and machine learning for intra-patient tissue samples. Area under the curve (AUC) of the receiver-operator characteristic was used as the main evaluation metric. Additionally, the performance was estimated in mm increments circumferentially from the tumor-normal margin. In intra-patient experiments, HSI classified conventional SCC with an AUC of 0.82 up to 3 mm from the cancer margin, which was more accurate than proflavin dye and autofluorescence (both p < 0.05). Intra-patient autofluorescence imaging detected human papilloma virus positive (HPV+) SCC with an AUC of 0.99 at 3 mm and greater accuracy than proflavin dye (p < 0.05). The inter-patient results showed that reflectance-based HSI and autofluorescence imaging outperformed proflavin dye and standard red, green, and blue (RGB) images (p < 0.05). In new patients, HSI detected conventional SCC in the larynx, oropharynx, and nasal cavity with 0.85–0.95 AUC score, and autofluorescence imaging detected HPV+ SCC in tonsillar tissue with 0.91 AUC score. This study demonstrates that label-free, reflectance-based HSI and autofluorescence imaging methods can accurately detect the cancer margin in ex-vivo specimens within minutes. This non-ionizing optical imaging modality could aid surgeons and reduce inadequate surgical margins during SCC resections.
Cell Heterogeneity and Phenotypic Plasticity in Metastasis Formation: The Case of Colon Cancer
Authors: Teeuwssen Miriam , Fodde Riccardo
Published: September 2019

The adenoma-to-carcinoma progression in colon cancer is driven by a sequential accumulation of genetic alterations at specific tumor suppressors and oncogenes. In contrast, the multistage route from the primary site to metastasis formation is underlined by phenotypic plasticity, i.e., the capacity of disseminated tumor cells to undergo transiently and reversible transformations in order to adapt to the ever-changing environmental contexts. Notwithstanding the considerable body of evidence in support of the role played by epithelial-to-mesenchymal transition (EMT)/mesenchymal-to-epithelial transition (MET) in metastasis, its rate-limiting function, the detailed underlying cellular and molecular mechanisms, and the extension of the necessary morphologic and epigenetic changes are still a matter of debate. Rather than leading to a complete epithelial or mesenchymal state, the EMT/MET-program generates migrating cancer cells displaying intermediate phenotypes featuring both epithelial and mesenchymal characteristics. In this review, we will address the role of colon cancer heterogeneity and phenotypic plasticity in metastasis formation and the contribution of EMT to these processes. The alleged role of hybrid epithelial/mesenchymal (E/M) in collective and/or single-cell migration during local dissemination at the primary site and more systemic spreading will also be highlighted.
MicroRNA Regulation of the Autotaxin-Lysophosphatidic Acid Signaling Axis
Authors: Murph Mandi M.
Published: September 2019

The revelation that microRNAs (miRNAs) exist within the human genome uncovered an underappreciated mechanism of gene expression. For cells to regulate expression of their genes, miRNA molecules and argonaute proteins bind to mRNAs and interfere with efficient translation of the RNA transcript. Although miRNAs have important roles in normal tissues, miRNAs may adopt aberrant functions in malignant cells depending on their classification as either a tumor suppressor or oncogenic miRNA. Within this review, the current status of miRNA regulation is described in the context of signaling through the lysophosphatidic acid receptors, including the lysophosphatidic acid-producing enzyme, autotaxin. Thus far, research has revealed miRNAs that increase in response to lysophosphatidic acid stimulation, such as miR-21, miR-30c-2-3p, and miR-122. Other miRNAs inhibit the translation of lysophosphatidic acid receptors, such as miR-15b, miR-23a, and miR200c, or proteins that are downstream of lysophosphatidic acid signaling, such as miR-146 and miR-21. With thousands of miRNAs still uncharacterized, it is anticipated that the complex regulation of lysophosphatidic acid signaling by miRNAs will continue to be elucidated. RNA-based therapeutics have entered the clinic with enormous potential in precision medicine. This exciting field is rapidly emerging and it will be fascinating to witness its expansion in scope.
Direct Intercellular Communications and Cancer: A Snapshot of the Biological Roles of Connexins in Prostate Cancer
Authors: Asencio-Barría Catalina , Defamie Norah , Sáez Juan C. , Mesnil Marc , Godoy Alejandro S.
Published: September 2019

Tissue homeostasis is the result of a complex intercellular network controlling the behavior of every cell for the survival of the whole organism. In mammalian tissues, cells do communicate via diverse long- and short-range communication mechanisms. While long-range communication involves hormones through blood circulation and neural transmission, short-range communication mechanisms include either paracrine diffusible factors or direct interactions (e.g., gap junctions, intercellular bridges and tunneling nanotubes) or a mixture of both (e.g., exosomes). Tumor growth represents an alteration of tissue homeostasis and could be the consequence of intercellular network disruption. In this network, direct short-range intercellular communication seems to be particularly involved. The first type of these intercellular communications thought to be involved in cancer progression were gap junctions and their protein subunits, the connexins. From these studies came the general assumption that global decreased connexin expression is correlated to tumor progression and increased cell proliferation. However, this assumption appeared more complicated by the fact that connexins may act also as pro-tumorigenic. Then, the concept that direct intercellular communication could be involved in cancer has been expanded to include new forms of intercellular communication such as tunneling nanotubes (TNTs) and exosomes. TNTs are intercellular bridges that allow free exchange of small molecules or even mitochondria depending on the presence of gap junctions. The majority of current research shows that such exchanges promote cancer progression by increasing resistance to hypoxia and chemotherapy. If exosomes are also involved in these mechanisms, more studies are needed to understand their precise role. Prostate cancer (PCa) represents a type of malignancy with one of the highest incidence rates worldwide. The precise role of these types of direct short-range intercellular communication has been considered in the progression of PCa. However, even though data are in favor of connexins playing a key role in PCa progression, a clear understanding of the role of TNTs and exosomes is needed to define their precise role in this malignancy. This review article summarizes the current view of the main mechanisms involved in short-range intercellular communication and their implications in cancer and delves into the biological, predictive and therapeutic role of connexins in PCa.
Reverse Water-Gas Shift Iron Catalyst Derived from Magnetite
Authors: Chou Chen-Yu , Loiland Jason A. , Lobo Raul F.
Published: September 2019

The catalytic properties of unsupported iron oxides, specifically magnetite (Fe3O4), were investigated for the reverse water-gas shift (RWGS) reaction at temperatures between 723 K and 773 K and atmospheric pressure. This catalyst exhibited a fast catalytic CO formation rate (35.1 mmol h−1 gcat.−1), high turnover frequency (0.180 s−1), high CO selectivity (>99%), and high stability (753 K, 45000 cm3h−1gcat.−1) under a 1:1 H2 to CO2 ratio. Reaction rates over the Fe3O4 catalyst displayed a strong dependence on H2 partial pressure (reaction order of ~0.8) and a weaker dependence on CO2 partial pressure (reaction order of 0.33) under an equimolar flow of both reactants. X-ray powder diffraction patterns and XPS spectra reveal that the bulk composition and structure of the post-reaction catalyst was formed mostly of metallic Fe and Fe3C, while the surface contained Fe2+, Fe3+, metallic Fe and Fe3C. Catalyst tests on pure Fe3C (iron carbide) suggest that Fe3C is not an effective catalyst for this reaction at the conditions investigated. Gas-switching experiments (CO2 or H2) indicated that a redox mechanism is the predominant reaction pathway.
One-Pot Solvent-Free Synthesis of N,N-Bis(2-Hydroxyethyl) Alkylamide from Triglycerides Using Zinc-Doped Calcium Oxide Nanospheroids as a Heterogeneous Catalyst
Authors: Kumar Dinesh , Park Chan Hee , Kim Cheol Sang
Published: September 2019

N,N-Bis(2-hydroxyethyl) alkylamide or fatty acid diethanolamides (FADs) were prepared from a variety of triglycerides using diethanolamine in the presence of different transition metal-doped CaO nanocrystalline heterogeneous catalysts. The Zn-doped Cao nanospheroids were found to be the most efficient heterogeneous catalyst, with complete conversion of natural triglycerides to fatty acid diethanolamide in 30 min at 90 °C. The Zn/CaO nanoparticles were recyclable for up to six reaction cycles and showed complete conversion even at room temperature. The amidation reaction of natural triglycerides was found to follow the pseudo-first-order kinetic model, and the first-order rate constant was calculated as 0.171 min−1 for jatropha oil aminolysis. The activation energy (Ea) and pre-exponential factor (A) for the same reaction were found to be 47.8 kJ mol−1 and 4.75 × 108 min−1, respectively.
Asymmetric Catalysis in Organic Synthesis
Authors: Adly Frady G. , Ghanem Ashraf
Published: September 2019

Biological systems, in most cases, recognize a pair of enantiomers as different substances eliciting different responses [...]
Improving Cancer Immunotherapy by Targeting the Hypoxic Tumor Microenvironment: New Opportunities and Challenges
Authors: Noman Muhammad Zaeem , Hasmim Meriem , Lequeux Audrey , Xiao Malina , Duhem Caroline , Chouaib Salem , Berchem Guy , Janji Bassam
Published: September 2019

Initially believed to be a disease of deregulated cellular and genetic expression, cancer is now also considered a disease of the tumor microenvironment. Over the past two decades, significant and rapid progress has been made to understand the complexity of the tumor microenvironment and its contribution to shaping the response to various anti-cancer therapies, including immunotherapy. Nevertheless, it has become clear that the tumor microenvironment is one of the main hallmarks of cancer. Therefore, a major challenge is to identify key druggable factors and pathways in the tumor microenvironment that can be manipulated to improve the efficacy of current cancer therapies. Among the different tumor microenvironmental factors, this review will focus on hypoxia as a key process that evolved in the tumor microenvironment. We will briefly describe our current understanding of the molecular mechanisms by which hypoxia negatively affects tumor immunity and shapes the anti-tumor immune response. We believe that such understanding will provide insight into the therapeutic value of targeting hypoxia and assist in the design of innovative combination approaches to improve the efficacy of current cancer therapies, including immunotherapy.
Effect of Adenomatous Polyposis Coli Loss on Tumorigenic Potential in Pancreatic Ductal Adenocarcinoma
Authors: Cole Jennifer M. , Simmons Kaitlyn , Prosperi Jenifer R.
Published: September 2019

Loss of the Adenomatous Polyposis Coli (APC) tumor suppressor in colorectal cancer elicits rapid signaling through the Wnt/β-catenin signaling pathway. In contrast to this well-established role of APC, recent studies from our laboratory demonstrated that APC functions through Wnt-independent pathways to mediate in vitro and in vivo models of breast tumorigenesis. Pancreatic ductal adenocarcinoma (PDAC) has an overall median survival of less than one year with a 5-year survival rate of 7.2%. APC is lost in a subset of pancreatic cancers, but the impact on Wnt signaling or tumor development is unclear. Given the lack of effective treatment strategies for pancreatic cancer, it is important to understand the functional implications of APC loss in pancreatic cancer cell lines. Therefore, the goal of this project is to study how APC loss affects Wnt pathway activation and in vitro tumor phenotypes. Using lentiviral shRNA, we successfully knocked down APC expression in six pancreatic cancer cell lines (AsPC-1, BxPC3, L3.6pl, HPAF-II, Hs 766T, MIA PaCa-2). No changes were observed in localization of β-catenin or reporter assays to assess β-catenin/TCF interaction. Despite this lack of Wnt/β-catenin pathway activation, the majority of APC knockdown cell lines exhibit an increase in cell proliferation. Cell migration assays showed that the BxPC-3 and L3.6pl cells were impacted by APC knockdown, showing faster wound healing in scratch wound assays. Interestingly, APC knockdown had no effect on gemcitabine treatment, which is the standard care for pancreatic cancer. It is important to understand the functional implications of APC loss in pancreatic cancer cells lines, which could be used as a target for therapeutics.
Empagliflozin Protects HK-2 Cells from High Glucose-Mediated Injuries via a Mitochondrial Mechanism
Authors: Lee Wen-Chin , Chau You-Ying , Ng Hwee-Yeong , Chen Chiu-Hua , Wang Pei-Wen , Liou Chia-Wei , Lin Tsu-Kung , Chen Jin-Bor
Published: September 2019

Empagliflozin is known to retard the progression of kidney disease in diabetic patients. However, the underlying mechanism is incompletely understood. High glucose induces oxidative stress in renal tubules, eventually leading to mitochondrial damage. Here, we investigated whether empagliflozin exhibits protective functions in renal tubules via a mitochondrial mechanism. We used human proximal tubular cell (PTC) line HK-2 and employed western blotting, terminal deoxynucleotidyl transferase dUTP nick end labelling assay, fluorescence staining, flow cytometry, and enzyme-linked immunosorbent assay to investigate the impact of high glucose and empagliflozin on cellular apoptosis, mitochondrial morphology, and functions including mitochondrial membrane potential (MMP), reactive oxygen species (ROS) production, and adenosine triphosphate (ATP) generation. We found that PTCs were susceptible to high glucose-induced mitochondrial fragmentation, and empagliflozin ameliorated this effect via the regulation of mitochondrial fission (FIS1 and DRP1) and fusion (MFN1 and MFN2) proteins. Empagliflozin reduced the high glucose-induced cellular apoptosis and improved mitochondrial functions by restoring mitochondrial ROS production, MMP, and ATP generation. Our results suggest that empagliflozin may protect renal PTCs from high glucose-mediated injuries through a mitochondrial mechanism. This could be one of the novel mechanisms explaining the benefits demonstrated in EMPA-REG OUTCOME trial.