bluuebunny/crossref_metadata_2025 huggingface dataset:
Annual Hazard Rate of Recurrence in Middle Eastern Papillary Thyroid Cancer over a Long-Term Follow-Up
Authors: Siraj Abdul K. , Parvathareddy Sandeep Kumar , Qadri Zeeshan , Siddiqui Khawar , Al-Sobhi Saif S. , Al-Dayel Fouad , Al-Kuraya Khawla S.
Published: December 2020

Predicting the pattern of recurrence in papillary thyroid cancer (PTC) is necessary to establish optimal surveillance and treatment strategies. We analyzed changes in hazard rate (HR) for tumor recurrence over time in 1201 unselected Middle Eastern PTC patients. The changes in risk were further analyzed according to clinical variables predictive of early (≤5 years) and late (>5 years) recurrence using Cox regression analysis to identify patient populations that remain at risk. Tumor recurrence was noted in 18.4% (221/1201) patients. The annualized hazard of PTC recurrence was highest during the first 5 years (2.8%), peaking between 1 and 2 years (3.7%), with a second smaller peak between 13 and 14 years (3.2%). Patients receiving radioactive iodine (RAI) therapy had lower recurrence hazard compared to those who did not (1.5% vs. 2.7%, p = 0.0001). Importantly, this difference was significant even in intermediate-risk PTC patients (0.7% vs. 2.3%; p = 0.0001). Interestingly, patients aged ≥55 years and having lymph node metastasis were at persistent risk for late recurrence. In conclusion, we confirmed the validity of the double-peaked time-varying pattern for recurrence risk in Middle Eastern PTC patients and our findings could help in formulating individualized treatment and surveillance plans.
Integrating Circulating Biomarkers in the Immune Checkpoint Inhibitor Treatment in Lung Cancer
Authors: Duchemann Boris , Remon Jordi , Naigeon Marie , Mezquita Laura , Ferrara Roberto , Cassard Lydie , Jouniaux Jean Mehdi , Boselli Lisa , Grivel Jonathan , Auclin Edouard , Desnoyer Aude , Besse Benjamin , Chaput Nathalie
Published: December 2020

Immune checkpoint inhibitors are now a cornerstone of treatment for non-small cell lung cancer (NSCLC). Tissue-based assays, such as Programmed cell death protein 1 (PD-L1) expression or mismatch repair deficiency/microsatellite instability (MMRD/MSI) status, are approved as treatment drivers in various settings, and represent the main field of research in biomarkers for immunotherapy. Nonetheless, responses have been observed in patients with negative PD-L1 or low tumor mutational burden. Some aspects of biomarker use remain poorly understood and sub-optimal, in particular tumoral heterogeneity, time-evolving sampling, and the ability to detect patients who are unlikely to respond. Moreover, tumor biopsies offer little insight into the host’s immune status. Circulating biomarkers offer an alternative non-invasive solution to address these pitfalls. Here, we summarize current knowledge on circulating biomarkers while using liquid biopsies in patients with lung cancer who receive treatment with immune checkpoint inhibitors, in terms of their potential as being predictive of outcome as well as their role in monitoring ongoing treatment. We address host biomarkers, notably circulating immune cells and soluble systemic immune and inflammatory markers, and also review tumor markers, including blood-based tumor mutational burden, circulating tumor cells, and circulating tumor DNA. Technical requirements are discussed along with the current limitations that are associated with these promising biomarkers.
Differential Gemcitabine Sensitivity in Primary Human Pancreatic Cancer Cells and Paired Stellate Cells Is Driven by Heterogenous Drug Uptake and Processing
Authors: Amrutkar Manoj , Vethe Nils Tore , Verbeke Caroline S. , Aasrum Monica , Finstadsveen Anette Vefferstad , Sántha Petra , Gladhaug Ivar P.
Published: December 2020

Gemcitabine resistance in pancreatic ductal adenocarcinoma (PDAC) is attributed to cancer cell-intrinsic drug processing and the impact of the tumor microenvironment, especially pancreatic stellate cells (PSCs). This study uses human PDAC-derived paired primary cancer cells (PCCs) and PSCs from four different tumors, and the PDAC cell lines BxPC-3, Mia PaCa-2, and Panc-1, to assess the fate of gemcitabine by measuring its cellular uptake, cytotoxicity, and LC-MS/MS-based metabolite analysis. Expression analysis and siRNA-mediated knockdown of key regulators of gemcitabine (hENT1, CDA, DCK, NT5C1A) was performed. Compared to PSCs, both the paired primary PCCs and cancer cell lines showed gemcitabine-induced dose-dependent cytotoxicity, high uptake, as well as high and variable intracellular levels of gemcitabine metabolites. PSCs were gemcitabine-resistant and demonstrated significantly lower drug uptake, which was not influenced by co-culturing with their paired PCCs. Expression of key gemcitabine regulators was variable, but overall strong in the cancer cells and significantly lower or undetectable in PSCs. In cancer cells, hENT1 inhibition significantly downregulated gemcitabine uptake and cytotoxicity, whereas DCK knockdown reduced cytotoxicity. In conclusion, heterogeneity in gemcitabine processing among different pancreatic cancer cells and stellate cells results from the differential expression of molecular regulators which determines the effect of gemcitabine.
Silencing of VEGFR2 by RGD-Modified Lipid Nanoparticles Enhanced the Efficacy of Anti-PD-1 Antibody by Accelerating Vascular Normalization and Infiltration of T Cells in Tumors
Authors: Cho Riki , Sakurai Yu , Jones Haleigh Sakura , Akita Hidetaka , Hisaka Akihiro , Hatakeyama Hiroto
Published: December 2020

Despite the promising anticancer effects of immune checkpoint inhibitors, their low objective response rate remains to be resolved; thus, combination therapies have been investigated. We investigated the combination of an anti-programmed cell death 1 (aPD-1) monoclonal antibody with the knockdown of vascular endothelial factor receptor 2 (VEGFR2) on tumor endothelial cells to overcome resistance to immune checkpoint inhibitors and improve the objective response rate. The successful delivery of small interfering RNA to tumor endothelial cells was achieved by RGD peptide-modified lipid nanoparticles composed of a novel, pH-sensitive, and biodegradable ssPalmO-Phe. RGD-modified lipid nanoparticles efficiently induced the knockdown of VEGFR2 in tumor endothelial cells (TECs), which induced vascular normalization. The combination of a PD-1 monoclonal antibody with Vegfr2 knockdown enhanced CD8+ T cell infiltration into tumors and successfully suppressed tumor growth and improved response rate compared with monotherapy. Our combination approach provides a promising strategy to improve therapeutic outcomes in immune checkpoint inhibitor-resistant cancers.
Patient-Derived Tumor Organoids for Drug Repositioning in Cancer Care: A Promising Approach in the Era of Tailored Treatment
Authors: Vivarelli Silvia , Candido Saverio , Caruso Giuseppe , Falzone Luca , Libra Massimo
Published: December 2020

Malignancies heterogeneity represents a critical issue in cancer care, as it often causes therapy resistance and tumor relapse. Organoids are three-dimensional (3D) miniaturized representations of selected tissues within a dish. Lately, organoid technology has been applied to oncology with growing success and Patients Derived Tumor Organoids (PDTOs) constitute a novel available tool which fastens cancer research. PDTOs are in vitro models of cancer, and importantly, they can be used as a platform to validate the efficacy of anti-cancer drugs. For that reason, they are currently utilized in clinics as emerging in vitro screening technology to tailor the therapy around the patient, with the final goal of beating cancer resistance and recurrence. In this sense, PDTOs biobanking is widely used and PDTO-libraries are helping the discovery of novel anticancer molecules. Moreover, they represent a good model to screen and validate compounds employed for other pathologies as off-label drugs potentially repurposed for the treatment of tumors. This will open up novel avenues of care thus ameliorating the life expectancy of cancer patients. This review discusses the present advancements in organoids research applied to oncology, with special attention to PDTOs and their translational potential, especially for anti-cancer drug testing, including off-label molecules.
Adjuvant Radiation Therapy for Male Breast Cancer—A Rare Indication?
Authors: Forster Tobias , Köhler Clara , El Shafie Rami , Weykamp Fabian , König Laila , Arians Nathalie , Adeberg Sebastian , Michel Laura , Smetanay Katharina , Golatta Michael , Sohn Christof , Heil Jörg , Schneeweiss Andreas , Debus Jürgen , Hörner-Rieber Juliane
Published: December 2020

Due to its rarity, there are no randomized trials investigating the outcome of adjuvant radiotherapy in MBC. This study reports on patient and tumor characteristics of 41 consecutive MBC patients treated between 1990 and 2018 and on clinical outcomes after surgical resection of tumors and adjuvant radiotherapy of the chest wall or breast. Local control (LC), locoregional control (LRC), overall survival (OS), disease-free survival (DFS), and toxicity were evaluated. After a median follow-up of 80 months (95% CI: 14.6–213.8 months) there was only one recurrence, in a patient’s locoregional lymph nodes 17 months after start of radiotherapy, resulting in an LC rate of 100% at 5 years and a 5-year LRC rate of 97.4% (standard deviation (SD): 0.025). Five-year DFS and OS rates were 64.6% (SD: 0.085) and 57.2% (SD: 0.082), respectively. Adjuvant radiotherapy was tolerated well without high-grade (CTCAE grade > II) adverse events. After tumor resection and adjuvant radiotherapy, LC and LRC rates in MBC patients are excellent and comparable to results found for female breast cancer (FBC) patients. However, as patients are often diagnosed with locally advanced, higher-risk tumors, distant recurrences remain the major failure pattern.
Global Autozygosity Is Associated with Cancer Risk, Mutational Signature and Prognosis
Authors: Jiang Limin , Guo Fei , Tang Jijun , Leng Shuguan , Ness Scott , Ye Fei , Kang Huining , Samuels David C. , Guo Yan
Published: December 2020

Global autozygosity quantifies the genome-wide levels of homozygous and heterozygous variants. It is the signature of non-random reproduction, though it can also be driven by other factors, and has been used to assess risk in various diseases. However, the association between global autozygosity and cancer risk has not been studied. From 4057 cancer subjects and 1668 healthy controls, we found strong associations between global autozygosity and risk in ten different cancer types. For example, the heterozygosity ratio was found to be significantly associated with breast invasive carcinoma in Blacks and with male skin cutaneous melanoma in Caucasians. We also discovered eleven associations between global autozygosity and mutational signatures which can explain a portion of the etiology. Furthermore, four significant associations for heterozygosity ratio were revealed in disease-specific survival analyses. This study demonstrates that global autozygosity is effective for cancer risk assessment.
Container-Sized CO2 to Methane: Design, Construction and Catalytic Tests Using Raw Biogas to Biomethane
Authors: Gaikwad Rohit , Villadsen Sebastian N. B. , Rasmussen Jan Pihl , Grumsen Flemming Bjerg , Nielsen Lars Pleth , Gildert Gary , Møller Per , Fosbøl Philip Loldrup
Published: December 2020

Direct catalytic methanation of CO2 (from CO2/CH4 biogas mixture) to produce biomethane was conducted in a pilot demonstration plant. In the demonstration project (MeGa-StoRE), a biogas desulfurization process and thermochemical methanation of biogas using hydrogen produced by water electrolysis were carried out at a fully operational biogas plant in Denmark. The main objective of this part of the project was to design and develop a reactor system for catalytic conversion of CO2 in biogas to methane and feed biomethane directly to the existing natural gas grid. A process was developed in a portable container with a 10 Nm3/h of biogas conversion capacity. A test campaign was run at a biogas plant for more than 6 months, and long-time operation revealed a stable steady-state conversion of more than 90% CO2 conversion to methane. A detailed catalytic study was performed to investigate the high activity and stability of the applied catalyst.
Iron: An Essential Element of Cancer Metabolism
Authors: Hsu Myriam Y. , Mina Erica , Roetto Antonella , Porporato Paolo E.
Published: December 2020

Cancer cells undergo considerable metabolic changes to foster uncontrolled proliferation in a hostile environment characterized by nutrient deprivation, poor vascularization and immune infiltration. While metabolic reprogramming has been recognized as a hallmark of cancer, the role of micronutrients in shaping these adaptations remains scarcely investigated. In particular, the broad electron-transferring abilities of iron make it a versatile cofactor that is involved in a myriad of biochemical reactions vital to cellular homeostasis, including cell respiration and DNA replication. In cancer patients, systemic iron metabolism is commonly altered. Moreover, cancer cells deploy diverse mechanisms to increase iron bioavailability to fuel tumor growth. Although iron itself can readily participate in redox reactions enabling vital processes, its reactivity also gives rise to reactive oxygen species (ROS). Hence, cancer cells further rely on antioxidant mechanisms to withstand such stress. The present review provides an overview of the common alterations of iron metabolism occurring in cancer and the mechanisms through which iron promotes tumor growth.
The Role of Adrenoceptors in the Retina
Authors: Ruan Yue , Böhmer Tobias , Jiang Subao , Gericke Adrian
Published: December 2020

The retina is a part of the central nervous system, a thin multilayer with neuronal lamination, responsible for detecting, preprocessing, and sending visual information to the brain. Many retinal diseases are characterized by hemodynamic perturbations and neurodegeneration leading to vision loss and reduced quality of life. Since catecholamines and respective bindings sites have been characterized in the retina, we systematically reviewed the literature with regard to retinal expression, distribution and function of alpha1 (α1)-, alpha2 (α2)-, and beta (β)-adrenoceptors (ARs). Moreover, we discuss the role of the individual adrenoceptors as targets for the treatment of retinal diseases.
Focused Ultrasound-Induced Cavitation Sensitizes Cancer Cells to Radiation Therapy and Hyperthermia
Authors: Hu Shaonan , Zhang Xinrui , Unger Michael , Patties Ina , Melzer Andreas , Landgraf Lisa
Published: December 2020

Focused ultrasound (FUS) has become an important non-invasive therapy for solid tumor ablation via thermal effects. The cavitation effect induced by FUS is thereby avoided but applied for lithotripsy, support drug delivery and the induction of blood vessel destruction for cancer therapy. In this study, head and neck cancer (FaDu), glioblastoma (T98G), and prostate cancer (PC-3) cells were exposed to FUS by using an in vitro FUS system followed by single-dose X-ray radiation therapy (RT) or water bath hyperthermia (HT). Sensitization effects of short FUS shots with cavitation (FUS-Cav) or without cavitation (FUS) to RT or HT (45 °C, 30 min) were evaluated. FUS-Cav significantly increases the sensitivity of cancer cells to RT and HT by reducing long-term clonogenic survival, short-term cell metabolic activity, cell invasion, and induction of sonoporation. Our results demonstrated that short FUS treatment with cavitation has good potential to sensitize cancer cells to RT and HT non-invasively.
LKB1 Differently Regulates Adipogenesis in Intramuscular and Subcutaneous Adipocytes through Metabolic and Cytokine-Related Signaling Pathways
Authors: Xu Ziye , Zhou Yanbing , Nong Qiuyun , You Wenjing , Wang Liyi , Wang Yizhen , Shan Tizhong
Published: December 2020

Liver kinase B1 (LKB1) plays important and various roles in the differentiation and lipid metabolism of adipocytes. However, the current knowledge of the respective roles of LKB1 in subcutaneous fat (SCF) and intramuscular fat (IMF) adipocytes remains unclear. This study aimed to discover the different regulatory mechanisms of LKB1 in SCF and IMF adipocytes. We found that LKB1 overexpression inhibited adipogenesis in both SCF and IMF adipocytes, and SCF adipocytes were more sensitive to regulation by LKB1. Transcriptomics results showed that IMF adipocytes had many more differentially expressed genes (DEGs) than SCF adipocytes. Pathway analysis of the shared and distinct DEGs revealed that the main adipogenesis mechanism was similar between SCF and IMF adipocytes upon LKB1 overexpression, while regulatory and metabolic signaling pathways, such as MAPK, PPAR signaling pathways, were differently regulated by LKB1. Several cytokine-related pathways were only enriched in LKB1-overexpressing IMF adipocytes. Our study reveals different regulators and signaling pathways between SCF and IMF adipocytes under LKB1 overexpression, which may be potential targets to differentially control SCF and IMF deposition and improve our understanding of the regulatory mechanisms of IMF deposition.
Emerging Roles of PRDM Factors in Stem Cells and Neuronal System: Cofactor Dependent Regulation of PRDM3/16 and FOG1/2 (Novel PRDM Factors)
Authors: Leszczyński Paweł , Śmiech Magdalena , Parvanov Emil , Watanabe Chisato , Mizutani Ken-ichi , Taniguchi Hiroaki
Published: December 2020

PRDI-BF1 (positive regulatory domain I-binding factor 1) and RIZ1 (retinoblastoma protein-interacting zinc finger gene 1) (PR) homologous domain containing (PRDM) transcription factors are expressed in neuronal and stem cell systems, and they exert multiple functions in a spatiotemporal manner. Therefore, it is believed that PRDM factors cooperate with a number of protein partners to regulate a critical set of genes required for maintenance of stem cell self-renewal and differentiation through genetic and epigenetic mechanisms. In this review, we summarize recent findings about the expression of PRDM factors and function in stem cell and neuronal systems with a focus on cofactor-dependent regulation of PRDM3/16 and FOG1/2. We put special attention on summarizing the effects of the PRDM proteins interaction with chromatin modulators (NuRD complex and CtBPs) on the stem cell characteristic and neuronal differentiation. Although PRDM factors are known to possess intrinsic enzyme activity, our literature analysis suggests that cofactor-dependent regulation of PRDM3/16 and FOG1/2 is also one of the important mechanisms to orchestrate bidirectional target gene regulation. Therefore, determining stem cell and neuronal-specific cofactors will help better understanding of PRDM3/16 and FOG1/2-controlled stem cell maintenance and neuronal differentiation. Finally, we discuss the clinical aspect of these PRDM factors in different diseases including cancer. Overall, this review will help further sharpen our knowledge of the function of the PRDM3/16 and FOG1/2 with hopes to open new research fields related to these factors in stem cell biology and neuroscience.
Effect of High Pressure Spark Plasma Sintering on the Densification of a Nb-Doped TiO2 Nanopowder
Authors: Verchère Alexandre , Cottrino Sandrine , Fantozzi Gilbert , Mishra Shashank , Gaudisson Thomas , Blanchard Nicholas , Pailhès Stéphane , Daniele Stéphane , Le Floch Sylvie
Published: December 2020

Sintering under pressure by means of the spark plasma sintering (SPS) technique is a common route to reduce the sintering temperature and to achieve ceramics with a fine-grained microstructure. In this work, high-density bulk TiO2 was sintered by high pressure SPS. It is shown that by applying high pressure during the SPS process (76 to 400 MPa), densification and phase transition start at lower temperature and are accelerated. Thus, it is possible to dissociate the two densification steps (anatase then rutile) and the transition phase during the sintering cycle. Regardless of the applied pressure, grain growth occurs during the final stage of the sintering process. However, twinning of the grains induced by the phase transition is enhanced under high pressure resulting in a reduction in the crystallite size.
The Importance of Interfacial Tension in Emulsification: Connecting Scaling Relations Used in Large Scale Preparation with Microfluidic Measurement Methods
Authors: Schroën Karin , de Ruiter Jolet , Berton-Carabin Claire
Published: December 2020

This paper starts with short descriptions of emulsion preparation methods used at large and smaller scales. We give scaling relations as they are generally used, and focus on the central role that interfacial tension plays in these relations. The actual values of the interfacial tension are far from certain given the dynamic behavior of surface-active components, and the lack of measurement methods that can be applied to conditions as they occur during large-scale preparation. Microfluidic techniques are expected to be very instrumental in closing this gap. Reduction of interfacial tension resulting from emulsifier adsorption at the oil-water interface is a complex process that consists of various steps. We discuss them here, and present methods used to probe them. Specifically, methods based on microfluidic tools are of great interest to study short droplet formation times, and also coalescence behavior of droplets. We present the newest insights in this field, which are expected to bring interfacial tension observations to a level that is of direct relevance for the large-scale preparation of emulsions, and that of other multi-phase products.
Visual Perception, Fine Motor, and Visual-Motor Skills in Very Preterm and Term-Born Children before School Entry–Observational Cohort Study
Authors: Dathe Anne-Kathrin , Jaekel Julia , Franzel Julia , Hoehn Thomas , Felderhoff-Mueser Ursula , Huening Britta M.
Published: December 2020

Very preterm children (<32 weeks gestation at birth; VP) are at risk of developmental difficulties. Specific functional difficulties and delays in visual perception, fine motor, and visual-motor skills have received little research attention, although they are critical for daily life and school readiness. Our aim was to assess these skills in a contemporary cohort of 60 VP and 60 matched term-born children before school entry. We administered the Movement Assessment Battery for Children (M-ABC-2) and the Developmental Test of Visual Perception (DTVP-2). Linear and logistic regressions were run to test group differences in performance and rates of developmental delay in visual perception, fine motor, and visual-motor skills. Very preterm children had lower scores than term-born children in visual perception (β = −0.25; p = 0.006), fine motor (β = −0.44; p < 0.001), and visual-motor tasks (β = −0.46; p < 0.001). The rate of developmental delay (<−1 SD) was higher among VP in visual perception (odds ratio (OR) = 3.4; 95% confidence interval (CI 1.1–10.6)), fine motor (OR = 6.2 (2.4–16.0)), and visual-motor skills (OR = 13.4 (4.1–43.9)) than in term-born controls. VP children are at increased risk for clinically relevant developmental delays in visual perception, fine motor, and visual-motor skills. Following up VP children until preschool age may facilitate early identification and timely intervention.
Evaluation of Concrete Material Properties at Early Age
Authors: Obayes Osamah , Gad Emad , Pokharel Tilak , Lee Jessey , Abdouka Kamiran
Published: December 2020

This article investigates the development of the following material properties of concrete with time: compressive strength, tensile strength, modulus of elasticity, and fracture energy. These properties were determined at seven different hydration ages (18 h, 30 h, 48 h, 72 h, 7 days, 14 days, 28 days) for four pure cement concrete mixes totaling 336 specimens tested throughout the study. Experimental data obtained were used to assess the relationship of the above properties with the concrete compressive strength and how these relationships are affected with age. Further, this study investigates prediction models available in literature and recommendations are made for models that are found suitable for application to early age concrete. Results obtained indicate that the relationship between the splitting tensile strength and concrete compressive strength can be approximated with a power function between 0.7 and 0.8, and this correlation is not affected by age. Fracture energy of the concrete and modulus of elasticity values obtained in this study correlate well with the square root of the compressive strength and it was found that this relationship holds true for all hydration ages investigated in this paper. Inverse analysis on the wedge-splitting test was conducted to determine the direct tensile strength. Values of tensile strength obtained from the inverse analysis have been validated numerically by carrying out finite element analysis on the wedge split, and anchor pull-out tests. The ratio of the tensile strength obtained from the inverse analysis to the splitting tensile strength was found to be in the range of 0.5–0.9 and 0.7 on average.
Intraseasonal Precipitation Variability over West Africa under 1.5 °C and 2.0 °C Global Warming Scenarios: Results from CORDEX RCMs
Authors: Ogega Obed M. , Gyampoh Benjamin A. , Mistry Malcolm N.
Published: December 2020

This study assessed the performance of 24 simulations, from five regional climate models (RCMs) participating in the Coordinated Regional Climate Downscaling Experiment (CORDEX), in representing spatiotemporal characteristics of precipitation over West Africa, compared to observations. The top five performing RCM simulations were used to assess future precipitation changes over West Africa, under 1.5 °C and 2.0 °C global warming levels (GWLs), following the representative concentration pathway (RCP) 8.5. The performance evaluation and future change assessment were done using a set of seven ‘descriptors’ of West African precipitation namely the simple precipitation intensity index (SDII), the consecutive wet days (CWD), the number of wet days index (R1MM), the number of wet days with moderate and heavy intensity precipitation (R10MM and R30MM, respectively), and annual and June to September daily mean precipitation (ANN and JJAS, respectively). The performance assessment and future change outlook were done for the CORDEX–Africa subdomains of north West Africa (WA-N), south West Africa (WA-S), and a combination of the two subdomains. While the performance of RCM runs was descriptor- and subregion- specific, five model runs emerged as top performers in representing precipitation characteristics over both WA-N and WA-S. The five model runs are CCLM4 forced by ICHEC-EC-EARTH (r12i1p1), RCA4 forced by CCCma-CanESM2 (r1i1p1), RACMO22T forced by MOHC-HadGEM2-ES (r1i1p1), and the ensemble means of simulations made by CCLM4 and RACMO22T. All precipitation descriptors recorded a reduction under the two warming levels, except the SDII which recorded an increase. Unlike the WA-N that showed less frequency and more intense precipitation, the WA-S showed increased frequency and intensity. Given the potential impact that these projected changes may have on West Africa’s socioeconomic activities, adjustments in investment may be required to take advantage of (and enhance system resilience against damage that may result from) the potential changes in precipitation.
Algorithm of Information Embedding into Digital Images Based on the Chinese Remainder Theorem for Data Security
Authors: Evsutin Oleg , Dzhanashia Kristina
Published: December 2020

With the huge transfers of data involved in the modern world, it is both crucial and challenging to maintain the security of data. This paper proposes a novel algorithm of information embedding into digital images that could be used to protect confidential information. The presented algorithm makes use of the Chinese remainder theorem and adaptive embedding to achieve good imperceptibility along with the possibility of hiding a decent amount of confidential information. The algorithm is evaluated via computing experiments and evaluation results, as well as comparison with similar works, demonstrate good imperceptibility qualities of the proposed scheme.
Interferometric Study of the Stability of K2CoxNi(1−x)(SO4)2·6H2O (KCNSH) Mixed Crystal Faces Depending on the Composition and Flow Velocity of the Solution
Authors: Grebenev Vadim V. , Lyasnikova Maria S. , Kovalyov Sergey I. , Vasilyeva Natalia A. , Rudneva Elena B. , Manomenova Vera L. , Voloshin Alexey E.
Published: December 2020

The change in the surface morphology of the (001) and (110) faces of K2CoxNi(1−x)(SO4)2·6H2O (KCNSH) mixed crystal, depending on the supercooling solution, has been studied under various conditions by laser interferometry. The experiments were carried out in both static mode, by changing the solution composition, and in dynamic mode, by changing the flow velocity. The conditions for the onset and suppression of the reaction of the isomorphic replacement leading to the formation of mosaic micro-inhomogeneity in mixed crystals have been studied. The mechanism of the reaction of isomorphic replacement has been analyzed depending on the shift of the solution composition from the initial one. For the first time, it has been observed that the reaction of isomorphic replacement may occur through a layer-by-layer mechanism.
Seawater Acidification Affects Beta-Diversity of Benthic Communities at a Shallow Hydrothermal Vent in a Mediterranean Marine Protected Area (Underwater Archaeological Park of Baia, Naples, Italy)
Authors: Appolloni Luca , Zeppilli Daniela , Donnarumma Luigia , Baldrighi Elisa , Chianese Elena , Russo Giovanni , Sandulli Roberto
Published: December 2020

One of the most important pieces of climate change evidence is ocean acidification. Acidification effects on marine organisms are widely studied, while very little is known regarding its effects on assemblages’ β-diversity. In this framework, shallow hydrothermal vents within a Marine Protected Area (MPA) represent natural ecosystems acting as laboratory set-ups where the continuous carbon dioxide emissions affect assemblages with consequences that can be reasonably comparable to the effects of global water acidification. The aim of the present study is to test the impact of seawater acidification on the β-diversity of soft-bottom assemblages in a shallow vent field located in the Underwater Archeological Park of Baia MPA (Gulf of Naples, Mediterranean Sea). We investigated macro- and meiofauna communities of the ‘Secca delle fumose’ vent system in sites characterized by sulfurous (G) and carbon dioxide emissions (H) that are compared with control/inactive sites (CN and CS). Statistical analyses were performed on the most represented macrobenthic (Mollusca, Polychaeta, and Crustacea), and meiobenthic (Nematoda) taxa. Results show that the lowest synecological values are detected at H and, to a lesser extent, at G. Multivariate analyses show significant differences between hydrothermal vents (G, H) and control/inactive sites; the highest small-scale heterogeneities (measure of β-diversity) are detected at sites H and G and are mainly affected by pH, TOC (Total Organic Carbon), and cations concentrations. Such findings are probably related to acidification effects, since MPA excludes anthropic impacts. In particular, acidification markedly affects β-diversity and an increase in heterogeneity among sample replicates coupled to a decrease in number of taxa is an indicator of redundancy loss and, thus, of resilience capacity. The survival is assured to either tolerant species or those opportunistic taxa that can find good environmental conditions among gravels of sand.
Histological Changes Related to Symptomatic Improvement of Spontaneous Keloids Treated with a Low-Dosage Regimen of UVA-1 Phototherapy
Authors: Cuenca-Barrales Carlos , Aneiros-Fernández José , Pérez-López Israel , Rodríguez-Pérez Julia , Ruiz-Villaverde Ricardo , Espelt-Otero Jorge Luis
Published: December 2020

Keloids are a difficult-to-treat disease characterized by an imbalance in mechanisms of tissue reparation. We present the case of a middle-aged woman with spontaneous keloids which histologically and clinically improved after UVA-1 phototherapy treatment. There are few reported cases of keloids treated with high doses of UVA-1 phototherapy. We used a low-dosage regimen with a good response in only one cycle, which could diminish the risk of skin cancer development.
An Unexplored Pharmacologic/Diagnostic Strategy for Peri-Implantitis: A Protocol Proposal
Authors: Golub Lorne M. , Räisänen Ismo T. , Sorsa Timo , Preshaw Philip M.
Published: December 2020

Dental implants are widely utilized for the replacement of missing teeth and are increasingly being placed in patients with systemic diseases, as well as in those who are medically healthy. Furthermore, it is recognized that peri-implant mucositis and peri-implantitis are highly prevalent, affecting large numbers of patients with implants, and it is pertinent to consider whether there may be any systemic impact of these conditions, given that there are known links between periodontitis and a number of chronic inflammatory diseases. In this article, we propose that the potential systemic complications of peri-implant diseases should be investigated in future clinical research, together with studies to identify whether systemically-administered host modulation therapies (HMTs) may be of benefit in the treatment of peri-implant diseases. These “HMTs” may prove a useful adjunct to routinely employed debridement and disinfection protocols, as well as potentially being of benefit in reducing risks of systemic complications. We also consider the use of chair-side diagnostic tests for active matrix metalloproteinase-8 (aMMP-8) in the detection of peri-implant disease given the ability of such tests to detect active tissue breakdown associated with peri-implantitis and periodontitis before conventional clinical and radiographic measurements indicate pathologic changes. These novel diagnostic and therapeutic strategies are relevant to consider as they may improve the management of peri-implant disease (beyond local debridement procedures), especially in those patients in whom systemic inflammation might be of concern.
Semi-Automatic Tracking of Laser Speckle Contrast Images of Microcirculation in Diabetic Foot Ulcers
Authors: Mennes Onno A. , Selles Mark , van Netten Jaap J. , van Baal Jeff G. , Steenbergen Wiendelt , Slart Riemer H. J. A.
Published: December 2020

Foot ulcers are a severe complication of diabetes mellitus. Assessment of the vascular status of diabetic foot ulcers with Laser Speckle Contrast Imaging (LSCI) is a promising approach for diagnosis and prognosis. However, manual assessment during analysis of LSCI limits clinical applicability. Our aim was to develop and validate a fast and robust tracking algorithm for semi-automatic analysis of LSCI data. The feet of 33 participants with diabetic foot ulcers were recorded with LSCI, including at baseline, during the Post-Occlusive Reactive Hyperemia (PORH) test, and during the Buerger’s test. Different regions of interest (ROIs) were used to measure microcirculation in different areas of the foot. A tracking algorithm was developed in MATLAB to reposition the ROIs in the LSCI scans. Manual- and algorithm-tracking of all recordings were compared by calculating the Intraclass Correlation Coefficient (ICC). The algorithm was faster in comparison with the manual approach (90 s vs. 15 min). Agreement between manual- and algorithm-tracking was good to excellent during baseline (ICC = 0.896–0.984; p < 0.001), the PORH test (ICC = 0.790–0.960; p < 0.001), and the Buerger’s test (ICC = 0.851–0.978; p < 0.001), resulting in a tracking algorithm that delivers assessment of LSCI in diabetic foot ulcers with results comparable to a labor-intensive manual approach, but with a 10-fold workload reduction.
The Utility of Deep Learning in Breast Ultrasonic Imaging: A Review
Authors: Fujioka Tomoyuki , Mori Mio , Kubota Kazunori , Oyama Jun , Yamaga Emi , Yashima Yuka , Katsuta Leona , Nomura Kyoko , Nara Miyako , Oda Goshi , Nakagawa Tsuyoshi , Kitazume Yoshio , Tateishi Ukihide
Published: December 2020

Breast cancer is the most frequently diagnosed cancer in women; it poses a serious threat to women’s health. Thus, early detection and proper treatment can improve patient prognosis. Breast ultrasound is one of the most commonly used modalities for diagnosing and detecting breast cancer in clinical practice. Deep learning technology has made significant progress in data extraction and analysis for medical images in recent years. Therefore, the use of deep learning for breast ultrasonic imaging in clinical practice is extremely important, as it saves time, reduces radiologist fatigue, and compensates for a lack of experience and skills in some cases. This review article discusses the basic technical knowledge and algorithms of deep learning for breast ultrasound and the application of deep learning technology in image classification, object detection, segmentation, and image synthesis. Finally, we discuss the current issues and future perspectives of deep learning technology in breast ultrasound.
Early Detection of Pancreatic Cancer: Role of Biomarkers in Pancreatic Fluid Samples
Authors: Ideno Noboru , Mori Yasuhisa , Nakamura Masafumi , Ohtsuka Takao
Published: December 2020

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer-related deaths worldwide. Most patients with PDAC present with symptomatic, surgically unresectable disease. Therefore, the establishment of strategies for the early detection is urgently needed. Molecular biomarkers might be useful in various phases of a strategy to identify high-risk individuals in the general population and to detect high-risk lesions during intense surveillance programs combined with imaging modalities. However, the low sensitivity and specificity of biomarkers currently available for PDAC, such as carbohydrate 19-9 (CA19-9), contribute to the late diagnosis of this deadly disease. Although almost all classes of biomarker assays have been studied, most of them are used in the context of symptomatic diseases. Compared to other body fluids, pancreatic juice and duodenal fluid are better sources of DNA, RNA, proteins, and exosomes derived from neoplastic cells and have the potential to increase the sensitivity/specificity of these biomarkers. The number of studies using duodenal fluid with or without secretin stimulation for DNA/protein marker tests have been increasing because of the less-invasiveness in comparison to pancreatic juice collection by endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic ultrasound-guided fine needle aspiration (EUS-FNA). Genomic analyses have been very well-studied, and based on PDAC progression model, mutations detected in pancreatic juice/duodenal fluid seem to indicate the presence of microscopic precursors and high-grade dysplasia/invasive cancer. In addition to known proteins overexpressed both in precursors and PDACs, such as CEA and S100P, comprehensive proteomic analysis of pancreatic juice from patients with PDAC identified many proteins which were not previously described. A novel technique to isolate exosomes from pancreatic juice was recently invented and identification of exosomal microRNA’s 21 and 155 could be biomarkers for diagnosis of PDAC. Since many studies have explored biomarkers in fluid samples containing pancreatic juice and reported excellent diagnostic accuracy, we need to discuss how these biomarker assays can be validated and utilized in the strategy of early detection of PDAC.
Joint Assessment of Equilibrium and Neuromotor Function: A Validation Study in Patients with Fibromyalgia
Authors: Lomas-Vega Rafael , Rodríguez-Almagro Daniel , Peinado-Rubia Ana Belén , Zagalaz-Anula Noelia , Molina Francisco , Obrero-Gaitán Esteban , Ibáñez-Vera Alfonso Javier , Osuna-Pérez María Catalina
Published: December 2020

Objective: To develop and validate a tool for evaluating balance and neuromotor function in patients with fibromyalgia (FMS). Methods: Brainstorming, the nominal group technique, and pilot-testing were used to select a battery of 20 functional balance tests that were included in a screening tool. A total of 108 subjects (62 with fibromyalgia syndrome, 22 aged over 65 years, and 24 healthy subjects) participated in this validation study. Factor validity, internal consistency, the ability to discriminate between patients and healthy subjects, and concurrent validity with the Fibromyalgia Impact Questionnaire (FIQ), the Central Sensitization Inventory (CSI), the 12-Item Short-Form Health Survey (SF-12), and other tools for measuring balance, such as the Dizziness Handicap Inventory (DHI), the Activities-Specific Balance Confidence Scale (ABC-16), the Falls Efficacy Scale-International (FES-I), and posturographic parameters, were evaluated. Results: The factorial analysis extracted four factors that explained 70% of the variance. The Alpha Cronbach value was 0.928. Concurrent validity of the screening tool with respect to other tools was high, and the receiver operating characteristic (ROC) curve analysis showed an AUC value of 0.932 for discriminating between healthy and FMS subjects. Severe balance disorder related to head movements in FMS patients was found. Conclusion: The 20-item JAEN (Joint Assessment of Equilibrium and Neuro-motor Function) screening tool is a valid and reliable tool for assessing balance in patients with FMS.
Trans-Olecranon Fracture-Dislocations of the Elbow: A Systematic Review
Authors: Cho Chul-Hyun , Kim Du-Han , Na Sang Soo , Choi Byung-Chan , Kim Beom-Soo
Published: December 2020

The purpose of this study is to provide a systematic review of the definition, ideal surgical method, complications, and prognosis of trans-olecranon fracture dislocations. An electronic search was performed in the PubMed, EMBASE, Scopus, and MEDLINE databases. The eligibility criteria included retrospective clinical study and review article in subjects older than 18 years with trans-olecranon fracture dislocations. Trans-olecranon fracture dislocations are defined as fractures in which the stability of the ulnohumeral joint is lost due to the intra-articular fracture of the olecranon without disruption of the proximal radioulnar joint. The seven papers were included that met the eligibility criteria for the quantitative synthesis. Findings indicate that a pre-contoured plate was used in 88.3% of cases (68 of 77 reports), with no reports of complications, suggesting that the pre-contoured 3.5 mm plate is the first choice of treatment. Postoperative mean elbow range of motion for the flexion–extension arc was 121.1° and 146.5° for the pronation-supination arc. Methods for postoperative clinical scores included the Broberg/Morrey rating with a result of excellent or good in 82.9% of cases, the ASES score with a mean of 88.7, and the DASH score with a mean of 11.75. Complications included heterotopic ossification in 21.9% (23/105) of cases, arthrosis in 25.7% (27/105) of cases, nerve damage in 18.1% (19/105) of cases, and osteoarthritis in 14.3% (15/105). With better understanding of the mechanism of injury and proper diagnosis and treatment, findings of the current review suggest a positive outcome. PROSPERO registration No.: CRD42019126568.
RPAS Automatic ADS-B Based Separation Assurance and Collision Avoidance System Real-Time Simulation Results
Authors: Di Vito Vittorio , Torrano Giulia
Published: December 2020

Remotely piloted aircraft systems (RPAS) are increasingly becoming relevant actors that are flying through the airspace and will gain much more importance in the future. In order to allow for their safe integration with manned conventional traffic in non-segregated airspaces, in accordance with the overall air traffic management (ATM) paradigm, specific enabling technologies are needed. As is well known, the detect and avoid (DAA) technology is fundamental among the enabling technologies identified as crucial for RPAS integration into the overall ATM system. In the meantime, to support extended surveillance, the universal introduction of cooperative automatic dependent surveillance-broadcast (ADS-B) on-board aircraft is being increasingly implemented because it has the potential to allow for the coverage of the entire airspaces in remote areas not usually covered by conventional radar surveillance. In this paper, experimental results that were obtained through the real-time validation, with hardware and human in the loop (RTS-HIL) simulations, of an automatic ADS-B based separation assurance and collision avoidance system aimed to support RPAS automatic operations (as well as remote pilot decision making) are presented and discussed. In the paper, after an introductory outline of the concept of operations (ConOps) of the system and its architectural organization, in addition to basic information about the main system functionalities, a description of the tests that were carried out is reported, and the obtained results are described and discussed in order to emphasize the performance and limitations of the proposed system. In particular, the obtained quantitative performances are reported and commented on, and the feedback presented by pilots in order to improve the system, e.g., in terms of preferred typology of conflict resolution maneuver elaborated by the system, is described.
The Impact of International Sanctions on Russian Financial Markets
Authors: Sultonov Mirzosaid
Published: December 2020

Russia’s international comportment and geostrategic moves, particularly the invasion of Ukraine and the annexation of Crimea in 2014, caused a substantial change in its international economic and political relations. In response to Russia’s invasion, the United States of America, the European Union, and their allies imposed a series of sanctions. In this study, by applying an exponential generalized autoregressive conditional heteroscedasticity model to daily logarithmic returns of the ruble exchange rate and the closing price index of the Russian Trading System, we analyze how the returns and volatility of the exchange rate and the stock price index responded to the sanctions and oil price changes. The estimation results show that the sanctions have a significant positive short-term impact on exchange rate returns. Economic sanctions have a significant negative long-term impact on the returns and variance of the exchange rate and a significant positive long-term impact on the returns of the stock price index. Financial sanctions have a positive/negative long-term impact on the returns of the exchange rate/stock price index and a positive long-term impact on the variance of the exchange rate and the stock price index. Corporate sanctions have a positive long-term impact on exchange rate returns.
Does Level of Education Influence the Development of Adolescents’ Mindsets?
Authors: Glerum Jaap , Dimeska Angela , Loyens Sofie M. M. , Rikers Remy M. J. P.
Published: December 2020

In the present study, we compared the mindset of preuniversity students and (primary and secondary) vocational students. Participants comprised of 173 students attending preuniversity education and 101 students attending vocational education. All participants completed a mindset questionnaire. We expected, based on previous educational experiences, that preuniversity students would show on average a higher score (i.e., more of a growth mindset) than vocational students. Results indicated, however, that there was hardly any difference in mindset between vocational and preuniversity students. The mindset of adolescents is therefore not influenced by the level of education.