bluuebunny/crossref_metadata_2025 huggingface dataset:
Microbial Transformation of Curcumol by Aspergillus niger
Authors: Li-Xia Chen , Hui Zhang , Qian Zhao , Shi-Yu Yin , Zhong Zhang , Tian-Xian Li , Feng Qiu
Published: March 2019

Curcumol is a representative index component for the quality control of the essential oil of Curcuma wenyujin Y.H. Chen et C. Ling, an antivirus and anticancer drug in China. Microbial transformation of curcumol (1) by Aspergillus niger AS 3.739 yielded two products. Their structures were elucidated as 3α-hydroxycurcumol (2) and 3α-(4′-methoxy-succinyloxy)-curcumol (3) by extensive spectroscopic methods including 2D-NMR and HRESI-MS. Among them, 3 is a new compound. Esterification of the substrate with succinic acid is a novel reaction in the field of microbial transformation of natural products. Compound 2, the major transformation product of 1, was a high regio- and stereo-specific hydroxylation product and showed significant antiviral effects.
Antioxidant and Anti-inflammatory Compounds in the Popular Landscape Plant Berberis thunbergii var. atropurpurea
Authors: Zhang Chuan-Rui , Schutzki Robert E. , Nair Muraleedharan G.
Published: March 2019

Berberis thunbergii var. atropurpurea. DC is one of the popular landscape plants in the USA, but until now lacked report on its chemical composition and biological properties. In this study, the antioxidant and anti-inflammatory activities of the methanolic extract and pure isolates of B. thunbergii var. atropurpurea, Crimson Pygmy, roots were evaluated using established bioassay procedures. The methanolic extract gave an absorbance value of 0.44 at 250 μg/mL concentration in the MTT assay. In addition, the extract inhibited lipid peroxidation (LPO) by 93% and the cyclooxygenase enzymes, COX-1 by 54 and COX-2 by 34%, at 100 μg/mL concentration. Therefore, a bioactivity-guided purification was carried out yielding pure isolates, out of which compounds 3-6 inhibited LPO by 34-66% at 100 μg/mL concentration. Similarly, compounds 1-6 inhibited COX-1 and −2 by 24-65 and 23-43% at 25 μg/mL concentration, respectively. This is the first report of the chemical constituents and biological activities of this plant.
Antiplasmodial Activity of Compounds from the Surface Exudates of Senecio roseiflorus
Authors: Kerubo Leonidah Omosa , Midiwo Jacob Ogweno , Derese Solomon , Langat Moses K. , Akala Hosea M. , Waters Norman C. , Peter Martin , Heydenreich Matthias
Published: March 2019

From the surface exudates of Senecio roseiflorus fourteen known methylated flavonoids and one phenol were isolated and characterized. The structures of these compounds were determined on the basis of their spectroscopic analysis. The surface exudate and the flavonoids isolated showed moderate to good antiplasmodial activity with 5,4′-dihydroxy-7-dimethoxyflavanone having the highest activity against chloroquine-sensitive (D6) and resistant (W2) strains of Plasmodium falciparum, with IC50 values of 3.2 ± 0.8 and 4.4 ± 0.01 μg/mL respectively.
Antiproliferative Activity of epi-Cercosporin in Human Solid Tumor Cell Lines
Authors: Trigos Ángel , Espinoza César , Martínez Maricela , Márquez Olivia , León Leticia G. , Padrón José M. , Norte Manuel , Fernández José J.
Published: March 2019

From cultures of Cercospora piaropi, a phytopathogenic fungus isolated from symptomatic leaves of water hyacinth was obtained a red compound, which, according to the spectroscopic data, was epi-cercosporin. It showed in vitro antiproliferative activity against the panel of human solid tumor cells HBL-100, HeLa, SW1573 and WiDr. Cell cycle studies revealed that epi-cercosporin induces accumulation of cells in G2/M phase.
New Anthraquinone Derivatives from Geosmithia lavendula
Authors: Malak Lourin G. , Bishay Daoud W. , Abdel-Baky Afaf M. , Moharram Ahmed M. , Cutler Stephen J. , Ross Samir A.
Published: March 2019

A phytochemical study of Geosmithia lavendula Pitt led to the isolation of three new anthraquinones: 1-acetyl-2,4,6,8-tetrahydroxy-9,10-anthraquinone (1), 2-acetyl-1,4,5,7-tetrahydroxy-9,10-anthraquinone (2), and 1-acetyl-2,4,5,6,7-pentahydroxy-9,10-anthraquinone (3), as well as another new compound named didodecyl thiodipropionate (propionic acid, 3,3-sulfinyl di-1,1′-didodecyl ester) (4), along with ten known compounds: 1-acetyl-2,4,5,7-tetrahydroxy-9,10- anthraquinone (rhodolamprometrin) (5), 1-acetyl-2,4,5,7,8-pentahydroxy-9,10-anthraquinone (6), (22E)-ergosta-6,22-diene-3β,5α,8α-triol, p-hydroxybenzyl alcohol, oleic acid, D-mannitol, palmitic acid, stearic acid, cis-vaccenic acid and 2-decenal. The structures of the isolated metabolites were elucidated based on NMR spectroscopic and mass spectrometric data. Compound 1 exhibited moderate activity against methicillin resistant Staphylococcus aureus with an IC50 value of 16.1 μg/mL.
Pancreatic Lipase Inhibitory Activity of Cassiamin A, a Bianthraquinone from Cassia siamea
Authors: Kumar Dilip , Karmase Aniket , Jagtap Sneha , Shekhar Ruchi , Bhutani Kamlesh K
Published: March 2019

In continuation towards the discovery of potential antiobesity lead(s) from natural products, we have screened n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc) and methanol (MeOH) extracts of 33 Indian medicinal plants (200 extracts) for in vitro pancreatic lipase inhibitory activity. Of the screened extracts, the EtOAc extract of Cassia siamea roots showed 74.3±1.4% enzyme inhibition at 250 μg/mL concentration. Bioassay guided fractionation of the active extract afforded 6 known compounds viz. chrysophanol (1), physcion (2), emodin (3), cassiamin A (4), friedelin (5) and cycloart-25-en-3β,24-diol (6). These compounds were further evaluated for pancreatic lipase inhibitory activity. Cassiamin A (4), a bianthraquinone, was found to be most active with an IC50 value of 41.8±1.2 μM and compounds 2 and 5 were found to be moderate enzyme inhibitors. Results indicate the antiobesity potential of C. siamea through pancreatic lipase inhibition.
Determination of Organic Acids in Salicornia herbacea by Solid-phase Extraction Combined with Liquid Chromatography
Authors: Han Damian , Tian Minglei , Park Dong Wha , Row Kyung Ho
Published: March 2019

A solid-phase extraction (SPE) method for the determination of procatechuic acid, ferulic acid and caffeic acid in Salicornia herbacea L. (Hamcho) has been developed. The optimal conditions were obtained by using a C18 SPE cartridge. By using ethanol and acetonitrile /water/ trifluoracetic acid as washing and eluting solvents, most interfering compounds originating from the hamcho matrix were eliminated. The extracts were sufficiently clean to be directly injected into the HPLC for further chromatographic analysis. Good linearity was obtained from 0.1 to 200 μg/mL (r > 0.999) for procatechuic acid, 0.2 to 400 μg/mL (r > 0.999) for caffeic acid and 0.3 to 600 μg/mL (r > 0.999) for ferulic acid, with the relative standard deviations being less than 3.6%. The mean recoveries of procatechuic acid, ferulic acid and caffeic acid from hamcho were more than 79.2% and the detection limit (S/N = 3:1) was 0.02 μg/mL for procatechuic acid, 0.01 μg/mL for caffeic acid and 0.04 μg/mL for ferulic acid. This method is a viable alternative to the existing HPLC methods for analyzing the content of procatechuic acid, ferulic acid and caffeic acid in hamcho.
Glucosinolate Biosynthesis in Hairy Root Cultures of Broccoli (Brassica oleracea var. italica)
Authors: Kim Sun-Ju , Park Woo Tae , Uddin Md. Romij , Kim Yeon Bok , Nam Sang-Yong , Jho Kwang Hyun , Park Sang Un
Published: March 2019

Here we present previously unreported glucosinolate production by hairy root cultures of broccoli (B. oleracea var. italica). Growth media greatly influenced the growth and glucosinolate content of hairy root cultures of broccoli. Seven glucosinolates, glucoraphanin, gluconapin, glucoerucin, glucobrassicin, 4- methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin, were identified by analysis of the broccoli hairy root cultures. Both half and full strength B5 and SH media enabled the highest accumulation of glucosinolates. In most cases, the levels of glucosinolates were higher in SH and BS media. Among the 7 glucosinolates, the accumulation of neoglucobrassicin was very high, irrespective of growth medium. The neoglucobrassicin content was 7.4-fold higher in SH medium than 1/2 MS, in which its level was the lowest. The 1/2 B5 medium supported the production of the highest amounts of glucobrassicin and 4- methoxyglucobrassicin, the levels for which were 36.2- and 7.9- fold higher, respectively, than their lowest content in 1/2 MS medium. The 1/2 SH medium enabled the highest accumulation of glucoraphanin and gluconapin in the broccoli hairy root cultures, whose levels were 1.8- and 4.6-fold higher, respectively, than their lowest content in 1/2 MS medium. Our results suggest that hairy root cultures of broccoli could be a valuable alternative approach for the production of glucosinolate compounds.
Characterization of Volatile Components of Zingiber roseum Essential Oil Using Capillary GC on Modified Cyclodextrins
Authors: Pragadheesh VPPalayam S , Yadav Anju , Singh Manju , Chanotiya Chandan S
Published: March 2019

The essential oil from different parts of Zingiber roseum plants was extracted by hydrodistillation, and analyzed using enantio-GC, capillary-GC and GC-MS. Two chiral selectors, 6- tert-butyldimethylsilyl-2,3-diethyl-β-cyclodextrin (TBDE-β-CD), and 2,3,6-methyl-β-cyclodextrin (PM-β-CD) doped into 14% cyanopropylphenyl/ 86% dimethylpolysiloxane, and 35% diphenyl/ 65% dimethylpolysiloxane, respectively were compared in order to clarify the stereochemistry and enantioselectivity of terpenoids using chiral gas chromatography. The enantiomeric excess for (1R)-(+)-α-pinene, (1R)-(+)-β-pinene, and (R)-(+)-limonene were characteristic for the rhizome. In TBDE-β-cyclodextrin coated chiral columns, a significant increase in separation factor (a) for β-pinene, limonene, linalool and α-terpineol enantiomers was observed when compared with methyl substituted β-cyclodextrin. The increase in chain length of the alkyl substituents may be the possible cause for enantiomer separation in β-cyclodextrin cavity. In addition, enantioreversal of α-pinene enantiomers in 6-tert-butyldimethylsilyl-2,3-diethyl-β-cyclodextrin was noticed as a unique feature. The enantiomeric compositions of Z. roseum fruit and flower essential oils were similar, but, in contrast, the rhizome oil contained an entirely different composition. Therefore, these results aid in the authentication of the natural origin of Z. roseum essential oils.
Essential Oil of Flowers of Anaphalis contorta, an Aromatic and Medicinal Plant from India
Authors: Joshi Rajesh K.
Published: March 2019

The hydro-distilled essential oil of dried flowers of Anaphalis contorta Hook f. has been examined by means of gas chromatography-mass spectrometry (GC- MS). The oil constituents were identified according to their mass spectra and their relative retention indices determined on a non-polar stationary phase capillary column. Fifty-one constituents have been identified representing 97.0% of the total oil. The major constituents were β-caryophyllene (19.2%), γ- curcumene (17.5%), δ-cadinene (10.2%), labda-7,14-dien-13-ol (4.8%), epi-α-cadinol (4.3%), bulnesol (4.3%), α-cadinol (3.8%), β-bisabolol (3.7%) and labda-8,14-dien-13-ol (3.3%). The oil was rich in sesquiterpene hydrocarbons (60.5%), followed by oxygenated sesquiterpenes (26.6%), oxygenated diterpenes (8.1%) and diterpene hydrocarbons (1.8%).
Chemical Composition of Hypericum richeri subsp. grisebachii Essential Oil from Croatia
Authors: Jerković Igor , Marasović Maja , Marijanović Zvonimir , Pilepić Kroata Hazler , Maleš Željan , Miloš Mladen
Published: March 2019

The aerial parts of Hypericum richeri Vill. subsp. grisebachii (Boiss.) Nyman were collected from two different locations in Croatia and subjected to hydrodistillation. GC/FID and GC/MS analysis of the isolated essential oils revealed 64 compounds representing 94.7% and 98.2% of the total oils. Predominant constituents in both samples were: germacrene D (10.9%; 6.0%), bicyclogermacrene (4.7%; 3.5%), α-pinene (6.8%; 6.9%), β-pinene (8.1%; 5.1%), decanoic acid (4.5%; 6.8%), β-caryophyllene (3.3%; 7.5%), δ-cadinene (7.0%; 4.4%), spathulenol (6.0%; 9.5%) and tetracosane (3.1%; 5.8%). Comparison of both samples revealed similarity in the chemical composition with minor fluctuations of constituent percentages. The chemical profile of Croatian oils was in general similar to those reported for other geographic areas regarding major mono- and sesquiterpene constituents. However, spathulenol, 5-cadinene and bicyclogermacrene were more abundant in Croatian oils. The presence of decanoic acid (4.5%; 6.8%) in Croatian oils was the major difference between acids and fatty acids derivatives. Higher abundance of alkanes (particularly tetracosane and docosane) was also noticed.
Chemical Composition of the Essential Oils from Two Vietnamese Asarum Species: A. glabrum and A. cordifolium
Authors: Thai Tran Huy , Bazzali Ophélie , Hoi Tran Minh , Tuan Nguyen Anh , Tomi Félix , Casanova Joseph , Bighelli Ange
Published: March 2019

The essential oil isolated from Asarum cordifolium C. E. C. Fischer recently discovered in Vietnam, and A. glabrum Merr., an endangered species listed as vulnerable in the Red Data Book of Vietnam, have been analyzed by a combination of chromatographic and spectroscopic techniques including 13C NMR spectroscopy. The composition of A. cordifolium essential oil, investigated for the first time, was dominated by elemicin (82.5%). The essential oil isolated from A. glabrum contained safrole (41.9%) as its major component and was characterized by the diversity of phenylpropanoids contained in this oil (10 compounds).
Essential Oils from the Leaves of Six Medicinal Plants of Nigeria
Authors: Ogunwande Isiaka A. , Avoseh Nudewhenu O. , Flamini Guido , Hassan Alimot-Sadiat O. , Ogunmoye AbdulRazaq O. , Ogunsanwo Akindele O. , Yusuf Kamorudeen O. , Kelechi Atuonwu O. , Tiamiyu Zainab A. , Tabowei Godgift O.
Published: March 2019

The chemistry of Cassia siamea L., C. occidentalis L. (Fabaceae), Cnestis ferruginea Vahl ex DC (Connaraceae), Anthocleista djalonensis A. Chev (Loganiaceae), Solanum torvum Swartz and S. erianthum G. Don (Solanaceae) volatiles grown in Nigeria have been studied. The essential oils were obtained by hydrodistillation and analyzed by GC and GC-MS. The main compounds of C. siamea were (E)-geranyl acetone (5.8%), 1-octen-3-ol (5.8%), linalool (7.8%), iso-italicene (15.4%) and (E)-β-damascenone (11.0%). On the other hand, C. occidentalis consisted mainly of (E)-geranyl acetone (8.0%), hexahydrofarnesylacetone (24.0%) and (E)-phytol acetate (40.7%). The oil of C. ferruginea was comprised mainly of (E)-geranyl acetone (13.7%), (E)-α-ionone (9.5%), phytol (5.8%), pentadecanal (6.1%) and 1-octen-3-ol (5.5%). The main compounds of A. djalonensis were α-humulene (31.9%), β-caryophyllene (17.8%), humulene epoxide II (12.7%) and caryophyllene oxide (5.9%). The main volatiles of S. torvum were (E)-phytol acetate (38.7%), pentadecanal (25.3%) and (E)-geranyl acetone (5.0%). Apart from methyl salicylate (4.5%), tetradecanal (2.2%), 2-pentyl furan (1.8%), hexahydrofarnesylacetone (1.6%) and hexadecanal (1.1%), all other compounds were either present in trace quantity or in amounts less than 1%. On the other hand, α-humulene (46.6%) and β-caryophyllene (20.6%) were the compounds occurring in higher quantities in S. erianthum. The volatile oil contents of Cassia siamea, Cnestis ferruginea, Anthocleista djalonensis and Solanum torvum are being reported for the first time.
Comparative Study on In Vitro Activities of Citral, Limonene and Essential Oils from Lippia citriodora and L. alba on Yellow Fever Virus
Authors: Gómez Luz Angela , Stashenko Elena , Ocazionez Raquel Elvira
Published: March 2019

The aim of this study was to compare the antiviral activities in vitro of citral, limonene and essential oils (EOs) from Lippia citriodora and L. alba on the replication of yellow fever virus (YFV). Citral and EOs were active before and after virus adsorption on cells; IC50 values were between 4.3 and 25 μg/mL and SI ranged from 1.1 to 10.8. Results indicate that citral could contribute to the antiviral activity of the L. citriodora EO. Limonene was not active and seemed to play an insignificant role in the antiviral activity of the examined EOs.
Composition, Mosquito Larvicidal, Biting Deterrent and Antifungal Activity of Essential Oils of Different Plant Parts of Cupressus arizonica var. glabra (‘Carolina Sapphire’)
Authors: Ali Abbas , Tabanca Nurhayat , Demirci Betul , Baser K. Husnu Can , Ellis Jane , Gray Sandra , Lackey Brett R. , Murphy Christine , Khan Ikhlas A. , Wedge David E.
Published: March 2019

Essential oils obtained by hydrodistillation of female cones (FC), male cones (MC), needle-twigs (NT) and wood-bark (WB) of ‘Carolina Sapphire’ [Cupressus arizonica var glabra (Sudw.) Little] were analyzed simultaneously by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The main constituents of the investigated essential oils were as follows: FC: α-pinene 68.5% and myrcene 11.9%; MC: α-pinene 22.5%, epi- zonarene 9.9%, zonarene 6.9%, limonene 6.8% and cadina-3,5-diene 6.1%; NT: α-pinene 20.7%, epi-zonarene 10.4%, zonarene 7.6%, limonene 5.9% umbellulone 5.8% and cadina-3,5-diene 5.8%; WB: α-pinene 40.7%, limonene 3.2% and umbellulone 2.9%. Essential oils were evaluated for antifungal activity against the strawberry anthracnose causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. Only WB essential oil showed moderate activity when compared with the commercial antifungal standards. Carolina Sapphire essential oils exhibited biting deterrent activity at 10 pg/cm2 with Biting Deterrence Index (BDI) values of 0.64, 0.59, 0.65 and 0.72, for FC, MC, NT and WB, respectively, and were significantly lower (P≤0.05) than the synthetic insect repellent, DEET (N,N-diethyl-meta-toluamide), at 25 nmol/cm2 against Aedes aegypti. The biting deterrence of NT and WB oils at 100 μg/cm2 with BDI values of 1.04 and 1.01, respectively, were similar, whereas the activity of MC (BDI=0.88) and FC (BDI=0.62) essential oils was lower than DEET at 25 nmol/cm2. Essential oil from FC with LD50 of 33.7 ppm was most toxic against 1-day-old Ae. aegypti larvae at 24-h post treatment. Female cone essential oil was significantly more toxic than MC (LD50=53.6 ppm), NT (LD50=55.5 ppm) and WB (LD50=44.6 ppm) essential oils.
Composition of Cassia fistula Oil and its Antifungal Activity by Disrupting Ergosterol Biosynthesis
Authors: Irshad Md. , Ahmad Aijaz , Zafaryab Md. , Ahmad Farah , Manzoor Nikhat , Singh Man , Rizvi M. Moshahid A.
Published: March 2019

Cassia fistula oil was investigated for antifungal activities against standard and clinical isolates of Candida species. Gas chromatography coupled with mass spectrometric (GC-MS) analysis of C. fistula oil revealed the presence of antimicrobial compounds like β-sitosterol, stigmasterol, ergosterol, betulinic acid, lupeol, fucosterol, α-amyrin and friedelin. The minimum inhibitory concentration (MIC) of the pulp and seed oils ranged between 250-300 and 350-500 μg/mL respectively. Both oils also inhibited by ≥63.8% ergosterol bio-synthesis in Candida cell wall {fluconazole (standard) ≥89.1%)}. The MICs were significantly correlated with the ergosterol content decrease in the cell wall (Student's t test p ≤ 0.005). We can, therefore, conclude that active compounds are present in Cassia fistula oil that primarily target ergosterol biosynthesis in Candida cell wall.
Chemical Composition and Antibacterial Activity of Essential Oils from Myrcia alagoensis (Myrtaceae)
Authors: Silva Aline do N. , Uetanabaro Ana Paula T. , Lucchese Angélica M.
Published: March 2019

The chemical composition and antibacterial activity of essential oils obtained from fresh and dried leaves of Myrcia alagoensis O. Berg, collected in a secondary forest remnant in north-eastern Brazil, was compared. The essential oils were obtained by hydrodistillation from fresh and dried leaves, and analysed by GC/FID and GC/MS. The antimicrobial properties of the oils were investigated against five bacteria by determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The essential oils were rich in cyclic sesquiterpenes, such as germacrene B, with antibiotic action against Gram-positive and Gram-negative bacteria. The drying process after collection interfered with the chemical composition and antibacterial activity of the assessed samples.
New Bioactive Secondary Metabolites from Bornean Red Alga, Laurencia similis (Ceramiales)
Authors: Kamada Takashi , Vairappan Charles Santhanaraju
Published: March 2019

A Bornean red algal population of Laurencia simlis Nam et Saito was analyzed for its secondary metabolite composition. Seven compounds were identified: ent -1(10)-aristolen-9β-ol (1), (+)-aristolone (2), axinysone B (3), 9-aristolen-1α-ol (4), 2,3,5,6-tetrabromoindole (5), 1-methyl-2,3,5,6-tetrabromoindole (6), and 1-methyl-2,3,5-tribromoindole (7). Compound 1 was identified as a new optical isomer of 1(10)-aristolen-9β-ol. Compounds 1, 4 and 5 exhibited good antibacterial activity against antibiotic resistant clinical bacteria and cytotoxic effects against selected cancer cell lines.
Structures and Biological Activities of Typicosides A1, A2, B1, C1 and C2, Triterpene Glycosides from the Sea Cucumber Actinocucumis typica
Authors: Silchenko Alexandra S. , Kalinovsky Anatoly I. , Avilov Sergey A. , Andryjaschenko Pelageya V. , Dmitrenok Pavel S. , Martyyas Ekaterina A. , Kalinin Vladimir I. , Jayasandhya P. , Rajan Gigi C. , Padmakumar Krishna P.
Published: March 2019

Five new minor triterpene glycosides, typicosides A1 (1), A2 (2), B1 (3), C1 (4) and C2 (5), along with two known glycosides, intercedenside A and holothurin B3, have been isolated from the sea cucumber Actinocucumis typica. Structures of the glycosides were elucidated by 2D NMR spectroscopy and MS. Glycosides 1–5 are linear mono- and disulfated tetraosides differing from each other in both aglycone structures and monosaccharide composition of the carbohydrate chains. Typicosides A1 (1) and A2 (2) have identical monosulfated carbohydrate moieties with a xylose residue as the third monosaccharide unit and differ from each other in aglycon structures. Typicoside B1 (3) has glucose as the third monosaccharide residue. Typicosides C1 (4) and C2 (5) contain the same disulfated carbohydrate chains and differ from each other in structures of aglycone side chains. Antifungal activity of glycosides 1–5 against three species of fungi along with cytotoxic activity against mouse spleen lymphocytes and mouse Ehrlich carcinoma cells (ascite form), as well as hemolytic activities against mouse erythrocytes have been studied. All new glycosides, except for typicoside C1 (4), containing a hydroxy-group in the aglycone side chain, demonstrate rather strong hemolytic and cytotoxic activities.
7-Oxodioscin, a New Spirostan Steroid Glycoside from the Rhizomes of Dioscorea nipponica
Authors: Ali Zulfiqar , Smillie Troy J. , Khan Ikhlas A.
Published: March 2019

Nine glycosides of spirostan (1–7) and furostan (8 and 9) type steroids including a new compound, 7-oxodioscin (1), were isolated from the rhizomes of Dioscorea nipponica. The structure of 1 was established as (25 R)-3β -O-[(α-L-rhamnopyranosyl-(1→4)- O-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranosyl)oxy]spirost-5-en-7-one by extensive spectroscopic techniques including HRESI-TOFMS, 1D and 2D NMR, and chemical methods. Known compounds were elucidated as dioseptemloside G (2), (25 R)-dracaenoside G (3), orbiculatoside B (4), dioscin (5), progenin III (6), gracillin (7), (3β,22α,25 R)–26-(β-D-glucopyranosyloxy)-22-methoxyfurost-5-en-3-yl O-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside (8), and methylprotodioscin (9).
Antifouling Indole Alkaloids from Two Marine Derived Fungi
Authors: He Fei , Han Zhuang , Peng Jiang , Qian Pei-Yuan , Qi Shu-Hua
Published: March 2019

In order to find non-toxic antifouling natural products from marine microorganisms, the chemical constituents of two marine derived fungi Penicillium sp. and Aspergillus sydowii have been investigated under bio-guided fractionation. A new indolyl diketopiperazine compound, penilloid A (1), together with 15 known ones were isolated from these two strains. The structure of 1 was elucidated on the basis of NMR and mass spectra. Some alkaloids showed significant antifouling and antibacterial activities. The results indicate that indole alkaloids could be a potential antifouling agent resource.
Antibacterial Activity against Ralstonia solanacearum of Phenolic Constituents Isolated from Dragon's Blood
Authors: Wang Hui , Luo Ying , Dai Haofu , Mei Wenli
Published: March 2019

Ralstonia solanacearum is a worldwide and devastating plant pathogen infesting over 200 host species. Synthetic bactericides against the pathogen have only achieved limited success and always cause both crop contamination and environmental pollution. However, natural bactericides are effective for protecting cultivated crops from destruction by disease, without the adverse effects of chemical bactericides. In this paper, fifteen phenolic constituents from dragon's blood were screened for their antimicrobial activity against Ralstonia solanacearum, and all exhibited inhibitory activity. These compounds are potential leading compounds for the development of bactericides against wilt diseases caused by Ralstonia solanacearum.
Interaction of Dihydromyricetin and α-Amylase
Authors: Chen Lei , Wang Chao , Wei Qingyi , Ning Zhengxiang , Yuan Erdong
Published: March 2019

The interaction of dihydromyricetin (DMY) and α-amylase was investigated. The complex formed between DMY and α-amylase resulted in decreased antioxidant activity of DMY and the catalytic activity of the enzyme, as well as efficient quenching of the intrinsic fluorescence of α-amylase. An α-amylase molecule provides one binding site for a DMY molecule. These results will be useful for exploiting this compound to combat diseases efficiently.
Quantitative Analysis of Total Flavonoids and Total Phenolic Acids in Thirty Hypericum Taxa
Authors: Pilepić Kroata Hazler , Maleš Željan , Crkvenčić Maja
Published: March 2019

The content of total flavonoids and total phenolic acids in the aerial parts of thirty Hypericum taxa collected over two consecutive seasons was investigated by spectrophotometric methods. All taxa examined contained flavonoids and phenolic acids in different quantities, although the differences between species and year's harvest were not found to be significant. The quantity of total flavonoids ranged from 0.1 to 1.6%. The highest content of flavonoids was found in the samples of H. japonicum (1.6%) and H. perforatum (1.5%), while the samples of H. androsaemum and H. balearicum comprised the lowest flavonoid amount (0.13%). The content of total phenolic acids in the investigated samples was found to be between 1.1–10.4%. The samples of H. perforatum were found to contain the highest quantity of phenolic acids (10.4% and 10.2%), whereas the sample of H. linarifolium showed the lowest amount of phenolic acids (1.1%).
New Diarylheptanoids and a Hydroxylated Ottelione from Ottelia alismoides
Authors: Hoye Thomas R. , Ayyad Seif-Eldin N. , Beckord Hollie J. , Brown Susan G.
Published: March 2019

Ten new diarylheptanoids (2, 3, 4, 5a-d, 6, 7, and 8) have been isolated from an extract of Ottelia alismoides. The structures of these previously unknown metabolites were determined by NMR spectroscopic analysis. A previously unknown, hydroxylated analog of the known otteliones A and B (1a and 1b)–namely, 3a-hydroxyottelione (13)–was also isolated. The 1H NMR analysis of the Mosher esters of alcohols derived from otteliones A and B (S-17/R-17 and S-20/R-20) are also reported.
Evaluation of the in vivo Anti-inflammatory and Analgesic and in vitro Anti-cancer Activities of Curcumin and its Derivatives
Authors: Jacob James N. , Badyal Dinesh K. , Bala Suman , Toloue Masoud
Published: March 2019

Curcumin, obtained from turmeric, has several biological properties to make it a desirable template for drug development. A lipophilic derivative of curcumin, diacetyl curcumin (DAC) and a hydrophilic derivative, diglutaryl curcumin (DGC) were synthesized and their in vivo analgesic and anti-inflammatory activities were compared with those of curcumin and aspirin. The in vitro anti-cancer activities of curcumin and the two derivatives against three cell cancer lines were compared with those against a non-cancerous cell line. The inhibitory effects were comparable to each other and nearing that of curcumin while they showed low inhibitory effect towards the non-cancerous cell line. The mouse tail flick assay showed that curcumin, DAC and DGC increased latency time. DGC was most effective as an analgesic, even more so than aspirin. The maximum percentage effect (MPE) was highest with DGC at 3 hours. The carrageenan induced paw edema model indicated anti-inflammatory activity of all three curcumin formulations. The percentage inhibition of paw edema was maximum for DAC, followed by aspirin and curcumin.
Antioxidant and Cytoprotective Effects of an Ethanol Extract of Acalypha wilkesiana var. macafeana from Malaysia
Authors: Din Wardah M. , Chu Jessica , Clarke Garry , Jin Khoo T. , Bradshaw Tracey D. , Fry Jeff R. , Wiart Christophe
Published: March 2019

In the annals of biomedical theory perhaps no single class of natural product has enjoyed more ingenious speculation than antioxidants formally aimed at counteracting oxidative insults which are involved in the pathophysiology of Alzheimer's and Parkinson's disease, cancer, amyotrophic lateral sclerosis, skin ageing and wound healing. In pursuing our study of Malaysian traditional medicines with antioxidant properties, we became interested in Acalypha wilkesiana var. macafeana hort., used traditionally to heal wounds. To examine whether Acalypha wilkesiana var. macafeana hort. could suppress oxidation an ethanol extract was tested by conventional chemical in vitro assays i.e., ferric reducing antioxidant potential assay (FRAP), DPPH scavenging assay and beta-carotene bleaching (BCB) assay. To explore whether Acalypha wilkesiana var. macafeana hort. protected cells against oxidative injuries, we exposed human hepatocellular liver carcinoma (HepG2) cells to tert-butylhydroperoxide ( t-BHP). In all the aforementioned experiments, the ethanol extracts elicited potent antioxidant and cytoprotective activities. To gain a better understanding of the phytochemical nature of the antioxidant principle involved, five fractions (F1-F5) obtained from the ethanol extract were tested using FRAP, DPPH and BCB assays. Our results provided evidence that F5 was the most active fraction with antioxidant potentials equal to 2.090 ± 0.307 μg/mL, 0.532 ± 0.041 μg/mL, 0.032 ± 0.025 μg/mL in FRAP, DPPH and BCB assay, respectively. Interestingly, F5 protected HepG2 against t-BHP oxidative insults. To further define the chemical identity of the antioxidant principle, we first performed a series of phytochemical tests, followed by liquid-chromatography and mass spectrometry (LC/MS) profiling which showed that the major compound contained in F5 was geraniin. To the best of our knowledge, this is the first report showing that the wound healing property of Acalypha wilkesiana var. macafeana hort. is mediated by a geraniin containing extract. Furthermore, our data leads us to conclude that geraniin could be used as a potential pharmaceutical and/or cosmetic topical agent.
Potential Applications for Annona squamosa Leaf Extract in the Treatment and Prevention of Foodborne Bacterial Disease
Authors: Dholvitayakhun Achara , Trachoo Nathanon , Sakee Uthai , Cushnie T.P. Tim
Published: March 2019

Foodborne disease is a major public health problem. The present study examined Annona squamosa leaves, which are traditionally used to treat diarrhea and other infections, for their potential to be used in modern food safety or medicine. Active constituents were partially purified by ethanol extraction and column chromatography. MICs of the extract were 62.5 to 125 μg/mL against Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus, and 250 μg/mL against Campylobacter jejuni. In time-kill assays, 500 μg/mL of the extract reduced colony forming unit numbers of C. jejuni almost 10 000-fold within 12 hours. Similar decreases were seen against B. cereus, but over a longer time-frame. LC-MS analysis indicated the presence of reticuline and oxophoebine. Assessment of stability by MIC assay showed activity was heat-labile, with loss of activity greatest following high temperature treatments. Activity was relatively stable at refrigeration temperature. These results indicate A. squamosa has broad-spectrum but heat-labile activity against foodborne bacterial pathogens, and bactericidal activity against B. cereus and C. jejuni. This bactericidal activity is not sufficiently rapid for A. squamosa to be used as a food sanitizer, but the extract could potentially be developed as an additive for refrigerated foods, or a modern treatment for foodborne illness.
Genoprotective Effect of the Chinese Herbal Decoction Xiao Jian Zhong Tang
Authors: Szeto Yim-Tong , Cheng Ngok-Fung , Pak Sok-Cheon , Kalle Wouter
Published: March 2019

The Chinese herbal decoction formula Xiao Jian Zhong Tang (XJZT) is one of the classic formulas from the classic traditional Chinese medicine (TCM). Previous studies on XJZT found that it is effective for treating peptic ulcer, irritable bowel syndrome, functional gastroenteritis and similar psychosomatic disorders of the digestive organs. It has also been shown that all the herbs used in XJZT contain antioxidants. In this study, we investigated the in vitro DNA protection effect of the individual herb extracts and the whole formula. Water extract of the herbs and XJZT were used to pre-treat human lymphocytes. The lymphocytes were then exposed to hydrogen peroxide. The in vitro DNA protection effect of the herbs was investigated by comet assay. No DNA protective effect ( P <0.05) was found for individual herb extracts, but XJZT showed protection of human lymphocytic DNA upon oxidative stress ( P<0.05). The in vitro DNA protection effect of XJZT was conferred by the synergistic effect of the herbs, while the individual herbs had no such effect.
Chemical Composition of the Essential Oil of Baccharoides lilacina from India
Authors: Joshi Rajesh K.
Published: March 2019

The essential oil composition from the aerial parts of Baccharoides lilacina (Dalzell & A. Gibson) M. R. Almeida was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 41 compounds have been identified, representing 97.4% of the total oil. The main constituents were identified as β-caryophyllene (27.7%), epi-α-cadinol (25.1%), caryophyllene oxide (9.9%), α-muurolol (7.6%), α-cadinene (6.1%) and α-cadinol 4.5%). The oil was found to be rich in oxygenated sesquiterpenes (47.1%) and sesquiterpene hydrocarbons (46.2%).
Chemical Composition and Biological Activities of the Essential Oil from Artemisia herba-alba Growing Wild in Tunisia
Authors: Amri Ismail , De Martino Laura , Marandino Aurelio , Lamia Hamrouni , Mohsen Hanana , Scandolera Elia , De Feo Vincenzo , Mancini Emilia
Published: March 2019

Aromatic plants can interfere in the Mediterranean ecosystem, mainly by the introduction in the environment of volatile compounds. For this reason, we studied the chemical composition and the possible phytotoxic and antimicrobial activities of the essential oil extracted from leaves of Tunisian Artemisia herba-alba Asso. The chemical composition of the essential oil, obtained by hydrodistillation, was analyzed by GC and GC-MS. In all, 24 compounds were identified. The main components were camphor (39.1.%), chrysanthenone (15.0%) and cis-thujone (7.8%). The essential oil was evaluated for its in vitro phytotoxic activity against germination and initial radical growth of Raphanus sativus L., Lepidium sativum L., Sinapis arvensis L., Triticum durum L. and Phalaris canariensis L. seeds. The radicle elongation of the five seeds was affected to different extents by the oil, while germination was not affected. The oil, when tested against eight selected bacterial strains, showed low antimicrobial activity. The chemical composition of the oil of A. herba-alba can help in the chemosystematics of this complex genus. However, the recorded biological activities seem to be neither ecologically nor medicinally significant.
Therapeutic Effects of Glycyrrhizic Acid
Authors: Ming Lee Jia , Yin Adeline Chia Yoke
Published: March 2019

Glycyrrhizic acid (GA), belonging to a class of triterpenes, is a conjugate of two molecules, namely glucuronic acid and glycyrrhetinic acid. It is naturally extracted from the roots of licorice plants. With its more common uses in the confectionery and cosmetics industry, GA extends its applications as a herbal medicine for a wide range of ailments. At low appropriate doses, anti-inflammatory, anti-diabetic, antioxidant, anti-tumor, antimicrobial and anti-viral properties have been reported by researchers worldwide. This review summarizes the effects of GA on metabolic syndrome, tumorigenesis, microbes and viruses, oxidative stress, and inflammation, as well as the reported side effects of the drug.
Anti-melanogenesis Constituents from the Seaweed Dictyota Coriacea
Authors: Ko Ryeo Kyeong , Kang Min-Chul , Kim Sang Suk , Oh Tae Heon , Kim Gi-Ok , Hyun Chang-Gu , Hyun Jin Won , Lee Nam Ho
Published: March 2019

This study was conducted to identify the anti-melanogenesis constituents from a seaweed Dictyota coriacea (Holmes). Three known compounds, viz. 1,9-dihydroxycrenulide (1), epiloliolide (2) and D-mannitol (3), were isolated from the ethanol extract. The melanin synthesis inhibition activities were evaluated using B16F10 melanoma cells for the isolates. Compared with the positive control, arbutin, compounds 1 and 2 exhibited more potency, showing 27.8 and 22.6 % inhibition activities at a substrate concentration of 30 μg/mL. Our studies also indicate that these compounds are not cytotoxic. Hence, they might prove to be useful therapeutic agents for treating hyperpigmentation and effective components of whitening cosmetics.
Cytotoxicity of Meroterpenoids from Sargassum Siliquastrum against Human Cancer Cells
Authors: Lee Jung Im , Kwak Myoung K. , Park Hee Y. , Seo Youngwan
Published: March 2019

The cytotoxicity of the brown alga Sargassum siliquastrum on human cancer cells (AGS, HT-29, HT-1080, and MCF-7) was investigated. Bioassay-guided fractionation of the crude extracts showed that the 85% aq. methanol (MeOH) fraction was the most toxic. Seven known meroterpenoids (1-7) were isolated from this cytotoxic fraction. Each compound was evaluated for its cytotoxic effect on human cancer cells. Compounds 1, 2, and 4 showed strong cytotoxicity against AGS, HT-29, and HT-1080 cell lines, with IC50 values ranging from 0.5 to 5.7 μg/mL.
New Flavonol Triglycosides from the Leaves of Soybean Cultivars
Authors: Murai Yoshinori , Takahashi Ryoji , Rodas Felipe Rojas , Kitajima Junichi , Iwashina Tsukasa
Published: March 2019

Soybean (Glycine max) is a major crop in the world. Three new flavonol 3- O-glycosides, kaempferol 3- O-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside] (1), kaempferol 3- O-α-L-rhamnopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→6)-β-D-galactopyranoside] (4) and quercetin 3- O-β-D-glucopyranosyl-(1β2)-[α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside] (5) were isolated from the leaves of soybean cultivars, together with three known compounds, kaempferol 3- O-β-D-glucopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside] (2), kaempferol 3- O-β-D-glucopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside] (3) and quercetin 3- O-β-D-glucopyranosyl-(1→2)-[7α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside] (6), and also common flavonoids. The isolated compounds possess similar structures and high water solubility, and so it was hard to isolate them (in particular 5 and 6) with a normal preparative HPLC system. Their final purification was achieved by a preparative HPLC system equipped with a recycle device.
Melitidin: A Flavanone Glycoside from Citrus grandis ‘Tomentosa’
Authors: Zou Wei , Wang Yonggang , Liu Haibin , Luo Yulong , Chen Si , Su Weiwei
Published: March 2019

Citrus grandis ‘Tomentosa’ is a traditional Chinese medicine, used as an antitussive. In this research, melitidin, a flavanone glycoside, was isolated from this species for the first time by using chromatographic methods. The structure was confirmed through comprehensive analyses of its ultraviolet, infrared, 1H and 13C NMR, HMBC and HMQC spectroscopic and high-resolution mass spectrometric data. Meliditin showed a good antitussive effect on cough induced by citric acid in Guinea pig, suggesting that it was a contributor to the antitussive effect of C. grandis ‘Tomentosa’.
Two New Chalcones from the Flowers of Clerodendrum inerme
Authors: Shahabuddin Shaik Khadar , Munikishore Rachakunta , Trimurtulu Golakoti , Gunasekar Duvvuru , Deville Alexandre , Bodo Bernard
Published: March 2019

Two new chalcones, 3-hydroxy-3′,4′-dimethoxychalcone (1) and 3,2′-dihydroxy-3′,4′-dimethoxychalcone (2),were isolated from the flowers of Clerodendrum inerme (L.) Gaertn together with two known flavones, 7- O-methylwogonin (3) and eucalyptin (4). The structures of the new compounds 1 and 2 have been established by extensive 2D-NMR and ESI-TOFMS studies.
A Novel Phenolic Compound from Phyllanthus emblica
Authors: She Gaimei , Cheng Ruiyang , Sha Lei , Xu Yixia , Shi Renbin , Zhang Lanzhen , Guo Yajian
Published: March 2019

A new compound, mucic acid 3- O-gallate (1), was isolated from the fruit of Phyllanthus emblica L, together with 5 known compounds (2-6). Their structures were elucidated on the basis of spectroscopic analysis, including 1D and 2D NMR techniques, and by comparison with literature data.
Anti-austeric Activity of Phenolic Constituents of Seeds of Arctium lappa
Authors: Tezuka Yasuhiro , Yamamoto Keiichi , Awale Suresh , Li Feng , Yomoda Satoshi , Kadota Shigetoshi
Published: March 2019

From seeds of Arctium lappa L. (Asteraceae) we obtained arctigenin (1), arctiin (2), chlorogenic acid (3), 4,5-dicaffeoylquinic acid (4), 3,5-dicaffeoylquinic acid (5), 3,4-dicaffeoylquinic acid (6), matairesinol (11), isolappaol A (12), lappaol F (14), and lappaol B (15), together with 1:1 mixtures of isolappaol C (7) and lappaol C (8), arctignan E (9) and arctignan D (10), and 12 and lappaol A (13), while 3,3′,4′-tri- O-demethylarctigenin (16), 3,3′-di- O-demethyl-4′-dehydroxyarctigenin (17), and 3- O-demethylarctigenin (18) were obtained by anaerobic microbiological metabolism of 1. Then, we evaluated the in vitro preferential cytotoxic activity of these pure compounds and 1:1 mixtures, together with enterodiol (19) and enterolactone (20), against human pancreatic cancer PANC-1 cells in nutrient-deprived medium (NDM). Among them, 1 and 18 showed potent activity, with PC50 values of 1.75 and 4.38 μ M, respectively, while 11, 15, and 17 showed mild activity with PC50 values of 31.1, 30.9, and 38.7 μ/M, respectively. By comparing their structures and PC50 values, the following structural moieties could be concluded to be important for the preferential cytotoxicity of 1: 1) the 3-hydroxy-4-methoxyphenyl group at the 2-position on the γ-butyrolactone ring, 2) the less polar substituent at the 3-position on the γ-butyrolactone ring, and 3) the γ-butyrolactone ring.
Bioactive Lignans from the Leaves and Stems of Schisandra wilsoniana
Authors: Yang Guang-Yu , Wang Rui-Rui , Gao Zhong-Hua , Li Yin-Ke , Yang Liu-Meng , Li Xiao-Nian , Shang Shan-Zhai , Zheng Yong-Tang , Xiao Wei-Lie , Sun Han-Dong
Published: March 2019

Three new dibenzocyclooctadiene lignans, marlignans M–O (1–3), together two known ones, were isolated from the leaves and stems of Schisandra wilsoniana. The structures of 1–3 were elucidated by spectroscopic methods, including extensive 1D- and 2D-NMR techniques. Compound 2 showed anti-HIV-1 activity with an EC50 value of 5.82 μg/mL and a therapeutic index (TI) of more than 12.8. Compound 3 showed obvious bioactivity in inhibiting Epstein-Barr virus early antigen (EBV-EA) activation.
Two New Dihydrostilbenoid Glycosides Isolated from the Leaves of Litsea coreana and their Anti-inflammatory Activity
Authors: Tang Wenjian , Lu Weili , Cao Xiaoqing , Zhang Yilong , Zhang Hong , Lv Xiongwen , Li Jun
Published: March 2019

Two new dihydrostilbenoid glycosides, named 5-(2-phenylethyl)-3-hydroxyphenol-1- O-β-D-glucopyranoside (1) and 6-(2-phenylethyl)-2,4-dihydroxy benzoic acid-2- O-β-D-glucopyranoside (2), together with two known compounds, were isolated from the leaves of Litsea coreana Levl.. Their structures were established on the basis of NMR spectroscopic, MS and chemical data. Biological tests revealed that 1-3 exhibited moderate anti-inflammatory activity through an inhibitory effect on TNF-α, IL-1 production from RAW264.7 cell line activated with lipopolysaccharides (LPS).
UPLC-Q-TOF/MS Coupled with Multivariate Statistical Analysis as a Powerful Technique for Rapidly Exploring Potential Chemical Markers to Differentiate between Radix Paeoniae Alba and Radix Paeoniae Rubra
Authors: Luo Nian-cui , Ding Wen , Wu Jing , Qian Da-wei , Li Zhen-hao , Qian Ye-fei , Guo Jian-ming , Duan Jin-ao
Published: March 2019

To explore rapidly the potential chemical markers for differentiating Radix Paeoniae Alba and Radix Paeoniae Rubra, a method is proposed based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) coupled with multivariate statistical analysis. Batches of commercial samples were analyzed by UPLC-Q-TOF/MS. The datasets of tR-m/z pair, ion intensities and sample codes were further processed with orthogonal partial least squared discriminant analysis (OPLS-DA) to compare holistically the difference between these two kinds of samples. Then statistics were used to generate an S-plot, in which the variables (tR-m/z pair) contributing most to the difference were clearly depicted as points at the two ends of “S”, and the components correlated to these ions should be regarded as the chemical markers. The identities of the most changed markers can be identified by comparing the mass/UV spectra and retention times with those of reference compounds and/or tentatively assigned by matching empirical molecular formulae with those of known compounds published in the literature. Using this proposed approach, albflorin, paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, galloylalbiflorin and paeoniflorigenone were found to be the differentiating components for discrimination of Radix Paeoniae Alba and Radix Paeoniae Rubra. Moreover, paeoniflorin sulfonate and its isomer, isomaltopaeoniflorin sulfonate, were found to be the characteristic markers for all Radix Paeoniae Alba samples that were processed by sulfurdioxide gas fumigation. The results suggested that this newly established approach could be used to explore rapidly the potential chemical markers for herbs with similar chemical characteristics.
Antimicrobial Activity of Crude Methanolic Extract from Phyllanthus niruri
Authors: Ibrahim Darah , Hong Lim Sheh , Kuppan Ninthianantham
Published: March 2019

The antibacterial efficiency of the methanolic extract of Phyllanthus niruri Linn. was investigated against pathogenic bacteria responsible for common infections of skin, and urinary and gastrointestinal tracts. The extract demonstrated antibacterial activities against all the Gram-positive and Gram-negative bacteria tested. The results obtained suggested that at higher concentrations the extract would eradicate the growth of bacterial cells. The bacterial cells, after exposure to the extract, showed complete alteration in their morphology, followed by collapse of the cells beyond repair. The study revealed that the methanolic extract of P. niruri may be an effective antibacterial agent to treat bacterial infections since the extract exhibited significant antimicrobial potency, comparable with that of the standard antibiotic chloramphenicol.