bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementing Optimization Techniques in PSS Design for Multi-Machine Smart Power Systems: A Comparative Study
Authors: Sabo Aliyu , Odoh Theophilus , Shahinzadeh Hossien , Azimi Zahra , Moazzami Majid
Published: March 2023

This study performed a comparative analysis of five new meta-heuristic algorithms specifically adopted based on two general classifications; namely, nature-inspired, which includes artificial eco-system optimization (AEO), African vulture optimization algorithm (AVOA), gorilla troop optimization (GTO), and non-nature-inspired or based on mathematical and physics concepts, which includes gradient-based optimization (GBO) and Runge Kutta optimization (RUN) for optimal tuning of multi-machine power system stabilizers (PSSs). To achieve this aim, the algorithms were applied in the PSS design for a multi-machine smart power system. The PSS design was formulated as an optimization problem, and the eigenvalue-based objective function was adopted to improve the damping of electromechanical modes. The expressed objective function helped to determine the stabilizer parameters and enhanced the dynamic performance of the multi-machine power system. The performance of the algorithms in the PSS’s design was evaluated using the Western System Coordinating Council (WSCC) multi-machine power test system. The results obtained were compared with each other. When compared to nature-inspired algorithms (AEO, AVOA, and GTO), non-nature-inspired algorithms (GBO and RUN) reduced low-frequency oscillations faster by improving the damping of electromechanical modes and providing a better convergence ratio and statistical performance.
An Improved Sliding Mode Controller for MPP Tracking of Photovoltaics
Authors: Jamshidi Fatemeh , Salehizadeh Mohammad Reza , Yazdani Reza , Azzopardi Brian , Jately Vibhu
Published: March 2023

Maximum power point tracking (MPPT) through an effective control strategy increases the efficiency of solar panels under rapidly changing atmospheric conditions. Due to the nonlinearity of the I–V characteristics of the PV module, the Sliding Mode Controller (SMC) is considered one of the commonly used control approaches for MPPT in the literature. This paper proposed a Backstepping SMC (BSMC) method that ensures system stability using Lyapunov criteria. A fuzzy inference system replaces the saturation function, and a modified SMC is used for MPPT to ensure smooth behavior. The proposed Fuzzy BSMC (FBSMC) parameters are optimized using a Particle Swarm Optimization (PSO) approach. The proposed controller is tested through various case studies on account of MPP’s dependence on temperature and solar radiation. The controller performance is assessed in partial shading conditions as well. The simulation results show that less settling time, a small error, and enhanced power extraction capability are achieved by applying the PSO-based FBSMC approach compared to the conventional BSMC- and ABC-based PI control presented in previous research in different scenarios. Moreover, the proposed approach provides faster adaptation to temperature and solar radiation variation, ensuring faster convergence to the MPP. Finally, the robustness of the proposed controller is validated by providing variation within the system components. The result of the proposed controller clearly indicates the lowest value of RMSE measured between PV voltage and the reference voltage, as well as the RMSE between PV power and maximum power. The results also show that the proposed MPPT controller exhibits the highest dynamic efficiency and mean power.
Pool Boiling of Novec-649 on Inclined Microchannel
Authors: Kaniowski Robert
Published: March 2023

Significant amounts of heat flow can be removed with small temperature differences by boiling. This process is used in a variety of industries, e.g., cooling electronics, digital circuits, power sources, etc. Heat dissipation from equipment that generates significant heat flows involves the movement of thermal energy through a wall into a cooling fluid. In the present study, this mechanism was analysed during the boiling of Novec-649 fluid at atmospheric pressure. The heat transfer surfaces were samples made of copper with milled, parallel grooves with a depth of 0.3 mm and a width ranging from 0.2 to 0.5 mm in 0.1 mm increments for straight channels and channels inclined with respect to the vertical by 30° and 60°, respectively. The study was carried out from the onset of nucleate boiling, approximately q = 7 kWm−2 with a heat flux increase until the critical heat flux was reached. The maximum heat flux was 262 kWm−2 and the heat transfer coefficient was 19.4 kWm−2K−1, achieved for surfaces with straight microchannels. A maximum heat flux increased by 80% and a heat transfer coefficient twice as high compared to a smooth surface was obtained. The performance of the experiment can be deemed adequate, considering that it compares well with the correlation results of different authors.
Application of Variable-Order Fractional Calculus to the Modeling of Calendar Aging in Lithium-Ion Batteries
Authors: López-Villanueva Juan Antonio , Rodríguez-Iturriaga Pablo , Parrilla Luis , Rodríguez-Bolívar Salvador
Published: March 2023

Battery aging is one of the key challenges that electrochemical energy storage faces. Models for both cycling and calendar aging are valuable for quantitatively assessing their contribution to overall capacity loss. Since batteries are stored and employed under varying conditions of temperature and state of charge in their real-life operation, the availability of a suitable model to anticipate the outcome of calendar aging in lithium-ion batteries under dynamic conditions is of great interest. In this article, we extend a novel model to predict the capacity loss due to calendar aging by using variable-order fractional calculus. For this purpose, some theoretical difficulties posed by variable-order definitions are discussed and compared by applying them to fit experimental results with a multi-parameter optimization procedure. We show that employing a variable-order model allows for a significant improvement in accuracy and predictive ability with respect to its constant-order counterpart. We conclude that variable-order models constitute an interesting alternative for reproducing complex behavior in dynamical systems, such as aging in lithium-ion batteries.
An Energy-Efficient Data Aggregation Clustering Algorithm for Wireless Sensor Networks Using Hybrid PSO
Authors: Sharmin Sharmin , Ahmedy Ismail , Md Noor Rafidah
Published: March 2023

Extending the lifetime of wireless sensor networks (WSNs) and minimizing energy costs are the two most significant concerns for data transmission. Sensor nodes are powered by their own battery capacity, allowing them to perform critical tasks and interact with other nodes. The quantity of electricity saved from each sensor together in a WSN has been strongly linked to the network’s longevity. Clustering conserves the most power in wireless transmission, but the absence of a mechanism for selecting the most suitable cluster head (CH) node increases the complexity of data collection and the power usage of the sensor nodes. Additionally, the disparity in energy consumption can lead to the premature demise of nodes, reducing the network’s lifetime. Metaheuristics are used to solve non-deterministic polynomial (NP) lossy clustering problems. The primary purpose of this research is to enhance the energy efficiency and network endurance of WSNs. To address this issue, this work proposes a solution where hybrid particle swarm optimization (HPSO) is paired with improved low-energy adaptive clustering hierarchy (HPSO-ILEACH) for CH selection in cases of data aggregation in order to increase energy efficiency and maximize the network stability of the WSN. In this approach, HPSO determines the CH, the distance between the cluster’s member nodes, and the residual energy of the nodes. Then, ILEACH is used to minimize energy expenditure during the clustering process by adjusting the CH. Finally, the HPSO-ILEACH algorithm was successfully implemented for aggregating data and saving energy, and its performance was compared with three other algorithms: low energy-adaptive clustering hierarchy (LEACH), improved low energy adaptive clustering hierarchy (ILEACH), and enhanced PSO-LEACH (ESO-LEACH). The results of the simulation studies show that HPSO-ILEACH increased the network lifetime, with an average of 55% of nodes staying alive, while reducing energy consumption average by 28% compared to the other mentioned techniques.
SGAM-Based Analysis for the Capacity Optimization of Smart Grids Utilizing e-Mobility: The Use Case of Booking a Charge Session
Authors: García Moisés Antón , García Ana Isabel Martínez , Karatzas Stylianos , Chassiakos Athanasios , Ageli Olympia
Published: March 2023

The description of the functionality of a smart grid’s architectural concept, analyzing different Smart Grid (SG) scenarios without disrupting the smooth operation of the individual processes, is a major challenge. The field of smart energy grids has been increasing in complexity since there are many stakeholder entities with diverse roles. Electric Vehicles (EVs) can transform the stress on the energy grid into an opportunity to act as a flexible asset. Smart charging through an external control system can have benefits for the energy sector, both in grid management and environmental terms. A suitable model for analyzing and visualizing smart grid use cases in a technology-neutral manner is required. This paper presents a flexible architecture for the potential implementation of electromobility as a distributed storage asset for the grid’s capacity optimization by applying the Use Case and Smart Grid Architecture Model (SGAM) methodologies. The use case scenario of booking a charge session through a mobile application, as part of the TwinERGY Horizon 2020 project, is deployed to structure the SGAM framework layers and investigate the applicability of the SGAM framework in the integration of electromobility as a distributed storage asset into electricity grids with the objective of enhanced flexibility and decarbonization.
A Self-Powered Dual-Stage Boost Converter Circuit for Piezoelectric Energy Harvesting Systems
Authors: Haseeb Abdul , Edla Mahesh , Thabet Ahmed Mostafa , Deguchi Mikio , Kamran Muhammad
Published: March 2023

Miniaturised piezoelectric devices are emerging energy harvesting sources that are appropriate for various implantable and wearable applications. However, these piezoelectric devices exhibit considerable internal resistance due to their internal impedance, which leads to self-start and low-energy conversion failures. This paper describes a dual-stage boost converter circuit by facilitating self-powering features and boosting the low voltage harvested by the piezoelectric devices into dc. The proposed circuit comprises conversion stages of ac-dc and dc-dc in Stages I and II, respectively. In addition, the proposed circuit does not require employing the auxiliary circuits to generate the train pulses by triggering the bidirectional switches to envelop the current being stored in Stage II and kick-start the self-powered circuit for piezoelectric energy harvesting systems. Theoretical assumptions and control strategies were tested and verified with ideal and impedance input sources. The proposed circuit could convert a low voltage of 3 Vac into 19 Vdc. The maximum attained output power by the proposed circuit was 3.61 mW. The outcome depicted that the proposed circuit boosted the low voltage and outperformed the existing literature circuits in terms of output voltage and power.
Pyrolysis Oil Derived from Plastic Bottle Caps: Characterization of Combustion and Emissions in a Diesel Engine
Authors: Maithomklang Somkiat , Sukjit Ekarong , Srisertpol Jiraphon , Klinkaew Niti , Wathakit Khatha
Published: March 2023

Recycling used plastic can help reduce the amount of plastic waste generated. Existing methods, namely the process of pyrolysis, are chemical heating processes that decompose plastics in the absence of oxygen. This decomposes the plastics in a controlled environment in order to produce fuel from waste. The present study consequently investigated the physical and chemical properties of pyrolysis oil derived from plastic bottle caps (WPBCO) and the effects on the engine performance and emission characteristics of a diesel engine operating on WPBCO. The experiments were conducted with a single-cylinder diesel engine operating at a constant 1500 rpm under various engine loading conditions. The experimental results of the chemical properties of test fuels indicated that WPBCO and diesel fuels have similar functional groups and chemical components. In comparison, WPBCO has a lower kinematic viscosity, density, specific gravity, flash point, fire point, cetane index, and distillation behavior than diesel fuel. However, WPBCO has a high gross calorific value, which makes it a suitable replacement for fossil fuel. In comparison to diesel fuel, the use of WPBCO in diesel engines results in increased brake-specific fuel consumption (BSFC) and brake thermal efficiency (BTE) under all load conditions. The combustion characteristics of the engine indicate that the use of WPBCO resulted in decreased in-cylinder pressure (ICP), rate of heat release (RoHR), and combustion stability compared to diesel fuel. In addition, the combustion of WPBCO advances the start of combustion more strongly than diesel fuel. The use of WPBCO increased emissions of NOX, CO, HC, and smoke. In addition, the particulate matter (PM) analysis showed that the combustion of WPBCO generated a higher PM concentration than diesel fuel. When WPBCO was combusted, the maximum rate of soot oxidation required a lower temperature, meaning that oxidizing the soot took less energy and that it was easier to break down the soot.
Air Conditioning Operation Strategies for Comfort and Indoor Air Quality in Taiwan’s Elementary Schools
Authors: Chang Ling-Yi , Chang Tong-Bou
Published: March 2023

The Executive Yuan in Taiwan plans to install air-conditioning (A/C) in all elementary schools within two years. However, besides the associated energy consumption and environmental issues, the use of A/C will inevitably result in the doors and windows of the classroom being closed, which will increase the accumulation of carbon dioxide (CO2) within the classroom. An excessive indoor CO2 concentration can result in reduced cognitive performance and an impaired learning efficiency. Therefore, the moderate introduction of external air into the classroom is essential to increase the air exchange rate (AER) and reduce the CO2 concentration level. Accordingly, the present study conducts a numerical investigation into the effects of various A/C operation strategies on the CO2 concentration within the classroom given different proportions of students remaining in the classroom during the recess. Overall, the results indicate that the optimal usage strategy is to operate the A/C over the full school day (08:00~15:50 p.m.) in conjunction with a mechanical ventilation system providing a fresh air exchange rate of 5 l/s for every person in the room. However, the use of a mechanical ventilation system inevitably incurs an additional hardware and energy consumption. Thus, an alternative recommendation is also proposed, in which the windows are opened and the air conditioner is turned off at every recess and during the lunchtime period. It is shown that the resulting CO2 concentration in the classroom is still consistent with the Taiwan Environmental Protection Administration (EPA) regulations and the thermal comfort of the students is achieved for more than three-quarters of the school day.
The Hydrological and Mechanical Effects of Forests on Hillslope Soil Moisture Changes and Stability Dynamics
Authors: Wang Xinhao , Wang Yunqi , Ma Chao , Wang Yujie , Li Tong , Dai Zhisheng , Wang Lijuan , Qi Zihan , Hu Yue
Published: March 2023

Vegetation can play a crucial role in stabilizing slopes through their hydrological and mechanical properties, yet few studies have systematically compared their effects on soil moisture resistance and slope stability. To investigate this, four steep slopes covered by different forests were analyzed in terms of climatic conditions, soil moisture dynamics, root strength and soil physical properties. The results revealed that the roots of Phyllostachys pubescens forests had a higher number and were deeper than the main plant species in the other three forests. Although the root tensile strength of Phyllostachys pubescens was not the strongest, its additional cohesion contributed more to hillslope stability. In the other three forests, suction stress was the main factor contributing to hillslope stability. The soil moisture change rate in Phyllostachys pubescens was found to be the smallest among the four forests studied, indicating that it had the greatest rainfall interception ability. The stability of the slope land covered by shrub forest was found to be more variable than the other three lands in high temperature conditions. Through its soil moisture reducing ability, root characteristics and magnitude of safety factor, Phyllostachys pubescens was identified as a suitable species for slope stabilization in the study area. The findings of this work may provide useful insights for local forest management in terms of selecting suitable plant species to reduce shallow landslides.
Is It Time to Revisit the Definition of Serial Homicide? New Evidence and Theory
Authors: Williams D J
Published: March 2023

Historically, serial homicide has been defined in various ways by experts. Recently, there have been renewed efforts to arrive at a consensus definition, yet these efforts have not yet been resolved. At the heart of the controversy appears to be the prioritization of either qualitative definitional features, such as offenders’ intentions and motives, or more observable quantitative features, specifically a minimum threshold of completed murders. The present technical note briefly summarizes this controversy before considering new empirical and theoretical research developments. These developments support a definition that includes a three-victim minimum threshold of forensically linked murderers by the same person(s), occurring in separate events over time, wherein a primary motive is often personal gratification (leisure experience).
“What We Know and What We Do Not Know about Evolutionary Genetic Adaptation to High Altitude Hypoxia in Andean Aymaras”
Authors: Amaru Ricardo , Song Jihyun , Reading N. Scott , Gordeuk Victor R. , Prchal Josef T.
Published: March 2023

Three well-studied populations living at high altitudes are Tibetans, Andeans (Aymaras and Quechuas), and Ethiopians. Unlike Tibetans and Ethiopians who have similar hemoglobin (Hb) levels as individuals living at sea level, Aymara Hb levels increase when living at higher altitudes. Our previous whole genome study of Aymara people revealed several selected genes that are involved in cardiovascular functions, but their relationship with Hb levels was not elucidated. Here, we studied the frequencies of known evolutionary-selected variants in Tibetan and Aymara populations and their correlation with high Hb levels in Aymara. We genotyped 177 Aymaras at three different altitudes: 400 m (Santa Cruz), 4000 m (La Paz), and 5000 m (Chorolque), and correlated the results with the elevation of residence. Some of the Tibetan-selected variants also exist in Aymaras, but at a lower prevalence. Two of 10 Tibetan selected variants of EPAS1 were found (rs13005507 and rs142764723) and these variants did not correlate with Hb levels. Allele frequencies of 5 Aymara selected SNPs (heterozygous and homozygous) at 4000 m (rs11578671_BRINP3, rs34913965_NOS2, rs12448902_SH2B1, rs10744822_TBX5, and rs487105_PYGM) were higher compared to Europeans. The allelic frequencies of rs11578671_BRINP3, rs34913965_NOS2, and rs10744822_SH2B1 were significantly higher for Aymaras living at 5000 m than those at 400 m elevation. Variant rs11578671, close to the BRINP3 coding region, correlated with Hb levels in females. Variant rs34913965 (NOS2) correlated with leukocyte counts. Variants rs12448902 (SH2B1) and rs34913965 (NOS2) associated with higher platelet levels. The correlation of these SNPs with blood cell counts demonstrates that the selected genetic variants in Aymara influence hematopoiesis and cardiovascular effects.
Role of Genetic Thrombophilia Markers in Thrombosis Events in Elderly Patients with COVID-19
Authors: Fevraleva Irina , Mamchich Daria , Vinogradov Dmitriy , Chabaeva Yulia , Kulikov Sergey , Makarik Tatiana , Margaryan Vahe , Manasyan Georgiy , Novikova Veronika , Rachina Svetlana , Melkonyan Georgiy , Lytkina Karine
Published: March 2023

Thrombosis is an extremely dangerous complication in elderly patients with COVID-19. Since the first months of the pandemic, anticoagulants have been mandatory in treatment protocols for patients with COVID-19, unless there are serious contraindications. We set out to discover if genetic thrombophilia factors continue to play a triggering role in the occurrence of thrombosis in patients with COVID-19 with prophylactic or therapeutic anticoagulants. We considered the following genetic markers as risk factors for thrombophilia: G1691A in the FV gene, C677T and A1298C in the MTHFR gene, G20210A and C494T in the FII gene, and (−675) 4G/5G in the PAI-I gene. In a cohort of 176 patients, we did not obtain a reliable result indicating a higher risk of thrombotic complications when taking therapeutic doses of anticoagulants in carriers of genetic markers for thrombophilia except the C494T mutation in the FII gene. However, there was still a pronounced tendency to a higher incidence of thrombosis in patients with markers of hereditary thrombophilia, such as FV G1691A and FII G20210A mutations. The presence of the C494T (Thr165Met) allele in the FII gene in this group of patients showed a statistically significant effect of the mutation on the risk of thrombotic complications despite anticoagulant therapy.
SDHA Germline Mutations in SDH-Deficient GISTs: A Current Update
Authors: Schipani Angela , Nannini Margherita , Astolfi Annalisa , Pantaleo Maria A.
Published: March 2023

Loss of function of the succinate dehydrogenase complex characterizes 20–40% of all KIT/PDGFRA-negative GIST. Approximately half of SDH-deficient GIST patients lack SDHx mutations and are caused by a hypermethylation of the SDHC promoter, which causes the repression of SDHC transcription and depletion of SDHC protein levels through a mechanism described as epimutation. The remaining 50% of SDH-deficient GISTs have mutations in one of the SDH subunits and SDHA mutations are the most common (30%), with consequent loss of SDHA and SDHB protein expression immunohistochemically. SDHB, SDHC, and SDHD mutations in GIST occur in only 20–30% of cases and most of these SDH mutations are germline. More recently, germline mutations in SDHA have also been described in several patients with loss of function of the SDH complex. SDHA-mutant patients usually carry two mutational events at the SDHA locus, either the loss of the wild type allele or a second somatic event in compound heterozygosis. This review provides an overview of all data in the literature regarding SDHA-mutated GIST, especially focusing on the prevalence of germline mutations in SDH-deficient GIST populations who harbor SDHA somatic mutations, and offers a view towards understanding the importance of genetic counselling for SDHA-variant carriers and relatives.
Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Rapeseed (Brassica napus L.)
Authors: Dai Jing , Han Peipei , Walk Thomas C. , Yang Ling , Chen Liyu , Li Yinshui , Gu Chiming , Liao Xing , Qin Lu
Published: March 2023

Ammonium transporters (AMTs) are plasma membrane proteins mediating ammonium uptake and transport. As such, AMTs play vital roles in ammonium acquisition and mobilization, plant growth and development, and stress and pathogen defense responses. Identification of favorable AMT genotypes is a prime target for crop improvement. However, to date, systematic identification and expression analysis of AMT gene family members has not yet been reported for rapeseed (Brassica napus L.). In this study, 20 AMT genes were identified in a comprehensive search of the B. napus genome, 14 members of AMT1 and 6 members of AMT2. Tissue expression analyses revealed that the 14 AMT genes were primarily expressed in vegetative organs, suggesting that different BnaAMT genes might function in specific tissues at the different development stages. Meanwhile, qRT-PCR analysis found that several BnaAMTs strongly respond to the exogenous N conditions, implying the functional roles of AMT genes in ammonium absorption in rapeseed. Moreover, the rapeseed AMT genes were found to be differentially regulated by N, P, and K deficiency, indicating that crosstalk might exist in response to different stresses. Additionally, the subcellular localization of several BnaAMT proteins was confirmed in Arabidopsis protoplasts, and their functions were studied in detail by heterologous expression in yeast. In summary, our studies revealed the potential roles of BnaAMT genes in N acquisition or transportation and abiotic stress response and could provide valuable resources for revealing the functionality of AMTs in rapeseed.
Quantitative Assessment of Horizontal Ecological Compensation for Cultivated Land Based on an Improved Ecological Footprint Model: A Case Study of Jiangxi Province, China
Authors: Zhong Xiaoyong , Guo Dongyan , Li Hongyi
Published: March 2023

Cultivated land horizontal ecological compensation is an essential means of reconciling agricultural ecosystem protection and regional economic development. It is important to design a horizontal ecological compensation standard for cultivated land. Unfortunately, there are some defects in the existing quantitative assessments of horizontal cultivated land ecological compensation. In order to raise the accuracy of ecological compensation amounts, this study established an improved ecological footprint model based on the ecosystem service function, focused on estimating the value of ecosystem service function, ecological footprint, ecological carrying capacity, ecological balance index and ecological compensation values of cultivated land in all cities of Jiangxi province. It then analyzed the rationality of ecological compensation amounts in Jiangxi province, which is one of the 13 provinces of major grain-producing areas in China. The results show the following: (1) The total value of soil conservation service function, carbon sequestration and oxygen release service function and ecosystem service function in Jiangxi province showed a spatial distribution trend of “gradually increasing around Poyang Lake Basin”. (2) The cultivated land ecological deficit areas in Jiangxi province are Nanchang City, Jiujiang City and Pingxiang City; ecological surplus areas are Yichun City, Ji’an City and eight other cities; and there is an obvious “Spatial Agglomeration” phenomenon in ecological deficit and ecological surplus areas where ecological deficit areas are mainly concentrated in the northwest region of Jiangxi. (3) The amount needed to attain fair ecological compensation for cultivated land is 5.2 times the payment amount for cultivated land; this indicated there is larger arable land, a favorable condition for agricultural cultivation, and better supply capacity of ecosystem services in most of the cities of Jiangxi. (4) The compensation amount for cultivated land ecological surplus areas in Jiangxi province is generally higher than the cost of ecological protection, and its proportion in GDP, fiscal revenue and agriculture-related expenditure is significantly higher than that in ecological deficit areas; this indicated that the compensation value of cultivated land could play the driving role in the protective behavior for cultivated land. The results provide a theoretical and methodological reference for the construction of horizontal ecological compensation standards for cultivated land.
Which Factors Influence Running Gait in Children and Adolescents? A Narrative Review
Authors: Sudlow Anthony , Galantine Paul , Vercruyssen Fabrice , Peyrot Nicolas , Raymond Jean-Jacques , Duché Pascale
Published: March 2023

In recent years, running has dramatically increased in children and adolescents, creating a need for a better understanding of running gait in this population; however, research on this topic is still limited. During childhood and adolescence multiple factors exist that likely influence and shape a child’s running mechanics and contribute to the high variability in running patterns. The aim of this narrative review was to gather together and assess the current evidence on the different factors that influence running gait throughout youth development. Factors were classified as organismic, environmental, or task-related. Age, body mass and composition, and leg length were the most researched factors, and all evidence was in favour of an impact on running gait. Sex, training, and footwear were also extensively researched; however, whereas the findings concerning footwear were all in support of an impact on running gait, those concerning sex and training were inconsistent. The remaining factors were moderately researched with the exception of strength, perceived exertion, and running history for which evidence was particularly limited. Nevertheless, all were in support of an impact on running gait. Running gait is multifactorial and many of the factors discussed are likely interdependent. Caution should therefore be taken when interpreting the effects of different factors in isolation.
Prevalence of Diabetic Retinopathy and Use of Common Oral Hypoglycemic Agents Increase the Risk of Diabetic Nephropathy—A Cross-Sectional Study in Patients with Type 2 Diabetes
Authors: Luo Wei-Ming , Su Jing-Yang , Xu Tong , Fang Zhong-Ze
Published: March 2023

Objective: This study investigated the effect of amino acid metabolism on the risk of diabetic nephropathy under different conditions of the diabetic retinopathy, and the use of different oral hypoglycemic agents. Methods: This study retrieved 1031 patients with type 2 diabetes from the First Affiliated Hospital of Liaoning Medical University in Jinzhou, which is located in Liaoning Province, China. We conducted a spearman correlation study between diabetic retinopathy and amino acids that have an impact on the prevalence of diabetic nephropathy. Logistic regression was used to analyze the changes of amino acid metabolism in different diabetic retinopathy conditions. Finally, the additive interaction between different drugs and diabetic retinopathy was explored. Results: It is showed that the protective effect of some amino acids on the risk of developing diabetic nephropathy is masked in diabetic retinopathy. Additionally, the additive effect of the combination of different drugs on the risk of diabetic nephropathy was greater than that of any one drug alone. Conclusions: We found that diabetic retinopathy patients have a higher risk of developing diabetic nephropathy than the general type 2 diabetes population. Additionally, the use of oral hypoglycemic agents can also increase the risk of diabetic nephropathy.
TLIF Online Videos for Patient Education—Evaluation of Comprehensiveness, Quality, and Reliability
Authors: Heisinger Stephan , Huber Dominikus , Matzner Michael P. , Hiertz Helmut , Lampe Lukas Peter , Zagata Jakob , Aspalter Stefan , Radl Christian , Senker Wolfgang , Mair Georg , Grohs Josef G.
Published: March 2023

Over the last few decades, the number of lumbar interbody fusion surgeries performed has been constantly increasing, with transforaminal lumbar interbody fusion (TLIF) being one of the most common surgical techniques. Due to easy accessibility, patients frequently use YouTube to obtain information on health-related issues. Consequently, online video platforms may be a valuable tool for patient education. The aim of this study was to assess the quality, reliability, and comprehensiveness of online videos on TLIF. We screened 180 videos on YouTube, yielding a total of 30 videos that met the inclusion criteria. These videos were evaluated using Global Quality Scale, DISCERN reliability tool, and JAMA Benchmark Score, and assessed in regard to their comprehensiveness and coverage of relevant aspects. At the time of rating, the videos had between 9188 and 1,530,408 views and between 0 and 3344 likes. The median rater assessment for all videos was “moderate quality”. GQS and subjective grades showed a moderate to strong statistically significant association with views and likes. Considering this association of GQS and subjective grade with views and likes, these criteria could be used by laypersons to identify good-quality content. Nevertheless, there is an urgent need for peer-reviewed content that covers all of the relevant aspects.
Knowledge, Attitudes, and Practices of Hygiene and the Prevention of Trachoma in the Indigenous Population of the Colombian Amazon Vaupés Department
Authors: Trujillo-Trujillo Julián , Meza-Cárdenas Mónica , Sánchez Sol , Zamora Sara , Porras Alexandra , López de Mesa Clara , Bernal Parra Luz , Bernal Lizarazú María , Miller Hollman , Silva Juan
Published: March 2023

The Colombian program to end trachoma implements the component F of the SAFE strategy in the Vaupés department of the Amazon rainforest. Cultural, linguistic, and geographical barriers and the coexistence of an ancestral medical system demand the technical and sociocultural adaptation of this component. A cross-sectional survey combined with focus-group discussions to understand the knowledge, attitudes, and practices of the indigenous population related to trachoma was conducted in 2015. Of the 357 heads of households that participated, 45.1% associated trachoma with a lack of hygiene, and 94.7% associated the concept of hygiene with taking one or more body baths per day, using commercial or handcrafted soap. In total, 93% reported cleaning their children’s faces and eyes more often when they have conjunctivitis, but 66.1% also did this with clothes or towels in use, and 52.7% of people shared towels; in total, 32.8% indicated that they would use ancestral medicine to prevent and treat trachoma. The SAFE strategy in Vaupés requires an intercultural approach to facilitate stakeholder support and participation to promote general and facial hygiene, washing clothes with soap, and not sharing towels and clothes to dry and clean children’s faces for effective and sustainable elimination of trachoma as a public health problem. This qualitative assessment facilitated an intercultural approach locally and in other Amazonian locations.
Combining Deep Learning and Hydrological Analysis for Identifying Check Dam Systems from Remote Sensing Images and DEMs in the Yellow River Basin
Authors: Li Mengqi , Dai Wen , Fan Mengtian , Qian Wei , Yang Xin , Tao Yu , Zhao Chengyi
Published: March 2023

Identifying and extracting check dams is of great significance for soil and water conservation, agricultural management, and ecological assessment. In the Yellow River Basin, the check dam, as a system, generally comprises dam locations and dam-controlled areas. Previous research, however, has focused on dam-controlled areas and has not yet identified all elements of check dam systems. This paper presents a method for automatically identifying check dam systems from digital elevation model (DEM) and remote sensing images. We integrated deep learning and object-based image analysis (OBIA) methods to extract the dam-controlled area’s boundaries, and then extracted the location of the check dam using the hydrological analysis method. A case study in the Jiuyuangou watershed shows that the precision and recall of the proposed dam-controlled area extraction approach are 98.56% and 82.40%, respectively, and the F1 score value is 89.76%. The completeness of the extracted dam locations is 94.51%, and the correctness is 80.77%. The results show that the proposed method performs well in identifying check dam systems and can provide important basic data for the analysis of spatial layout optimization and soil and water loss assessment.
Profile of Maternal Mortality of COVID-19 in Ceará, in the Period of 2020
Authors: Francelino Sheyla Martins Alves , Bezerra Italla Maria Pinheiro , Alves Sabrina Alaide Amorim , Leitão Francisco Naildo Cardoso , de Deus Morais Mauro José , Ramos José Lucas Souza , da Rocha João Batista Francalino , Antunes Thaiany Pedrozo Campos , Smiderle Fabiana Rosa Neves , de Abreu Luíz Carlos
Published: March 2023

The aim of this paper is to describe the profile of maternal mortality of COVID-19 in the state of Ceará, Brazil, in the period 2020. Ecological, exploratory, cross-sectional study, with secondary data from the Influenza Epidemiological Surveillance Information System, were made available by the Obstetric Observatory Brazilian COVID-19. A total of 485 pregnant and postpartum women were included, and the analysis considered the notifications from the year 2020. The variables of interest and the outcome (death/cure by COVID-19) were analyzed in a descriptive way. Most pregnant and postpartum women were between 20 and 35 years old, brown/white skin color and residing in an urban area. The proportion of deaths was 5.8% in the year 2020. In that period, the rates of hospitalization in the ward increased by 95.5%, 12.6% of hospitalization in the Unit of Intensive Care (ICU), and 7.2% needed invasive ventilatory support. Maternal mortality from COVID-19 suggests an emergency in terms of the development of health actions and policies due to the aggravation and risks due to this disease.
Spatial Heterogeneity and Influencing Factors of High-Grade Tourist Attractions in the Tibetan Plateau
Authors: Shi Shanshan , Li Mi , Li Ziqiang , Xi Jianchao
Published: March 2023

The construction of the world tourism destination on the Tibetan Plateau is inseparable from the traditional tourist attractions, which are significant landscape ecological units. Based on the data of high-grade tourist attractions on the Tibetan Plateau, the spatial heterogeneity and influence factors are studied employing the Standard Deviation Ellipse (SDE), Kernel Density Estimation (KDE), spatial autocorrelation (SA), and modified tourism gravity model methods. The results show that: (1) the overall spatial distribution characteristic of high-grade tourist attractions is in the direction of northeast-southwest, with solid centripetal force, and the center of gravity of the ellipse is in Yushu City. (2) The spatial heterogeneity of the kernel density distribution is remarkable, clustered in the southeastern half of the plateau, showing a double nucleus-driven and strip-connected pattern. The distribution among cities has a hierarchical heterogeneity, and the two capital cities of Xining and Lhasa play a crucial role. (3) The high-grade tourist attractions are spatially dependent, with evident characteristics of large dispersion and small clustering, and the spatial association type is mainly negative. (4) This paper verifies the significant single-factor mechanism affecting the spatial distribution from supportive and intrinsic dimensions with natural environmental base, tourism resource endowment, socio-economic development, transportation location constraints, and spatial tourism linkages. Finally, the article provides suggestions for the high-quality development of high-grade tourist attractions on the Tibetan Plateau.
Twelve Tips for Inclusive Practice in Healthcare Settings
Authors: Marjadi Brahmaputra , Flavel Joanne , Baker Kirsten , Glenister Kristen , Morns Melissa , Triantafyllou Mel , Strauss Penelope , Wolff Brittany , Procter Alexandra Marie , Mengesha Zelalem , Walsberger Scott , Qiao Xiaoxi , Gardiner Paul A.
Published: March 2023

This paper outlines practical tips for inclusive healthcare practice and service delivery, covering diversity aspects and intersectionality. A team with wide-ranging lived experiences from a national public health association’s diversity, equity, and inclusion group compiled the tips, which were reiteratively discussed and refined. The final twelve tips were selected for practical and broad applicability. The twelve chosen tips are: (a) beware of assumptions and stereotypes, (b) replace labels with appropriate terminology, (c) use inclusive language, (d) ensure inclusivity in physical space, (e) use inclusive signage, (f) ensure appropriate communication methods, (g) adopt a strength-based approach, (h) ensure inclusivity in research, (i) expand the scope of inclusive healthcare delivery, (j) advocate for inclusivity, (k) self-educate on diversity in all its forms, and (l) build individual and institutional commitments. The twelve tips are applicable across many aspects of diversity, providing a practical guide for all healthcare workers (HCWs) and students to improve practices. These tips guide healthcare facilities and HCWs in improving patient-centered care, especially for those who are often overlooked in mainstream service provision.
Simvastatin Improves Benign Prostatic Hyperplasia: Role of Peroxisome-Proliferator-Activated Receptor-γ and Classic WNT/β-Catenin Pathway
Authors: Wang Zhen , Yang Shu , Li Yan , Zhou Yongying , Liu Daoquan , Liu Jianmin , DiSanto Michael E. , Zhang Xinhua
Published: March 2023

Benign prostatic hyperplasia (BPH) is a common disease in elderly men with an uncertain etiology and mechanistic basis. Metabolic syndrome (MetS) is also a very common illness and is closely related to BPH. Simvastatin (SV) is one of the widely used statins for MetS. Peroxisome-proliferator-activated receptor gamma (PPARγ), crosstalking with the WNT/β-catenin pathway, plays important roles in MetS. Our current study aimed to examine SV-PPARγ-WNT/β-catenin signaling in the development of BPH. Human prostate tissues and cell lines plus a BPH rat model were utilized. Immunohistochemical, immunofluorescence, hematoxylin and eosin (H&E) and Masson’s trichrome staining, construction of a tissue microarray (TMA), ELISA, CCK-8 assay, qRT-PCR, flow cytometry, and Western blotting were also performed. PPARγ was expressed in both prostate stroma and epithelial compartments and downregulated in BPH tissues. Furthermore, SV dose-dependently triggered cell apoptosis and cell cycle arrest at the G0/G1 phase and attenuated tissue fibrosis and the epithelial–mesenchymal transition (EMT) process both in vitro and in vivo. SV also upregulated the PPARγ pathway, whose antagonist could reverse SV produced in the aforementioned biological process. Additionally, crosstalk between PPARγ and WNT/β-catenin signaling was demonstrated. Finally, correlation analysis with our TMA containing 104 BPH specimens showed that PPARγ was negatively related with prostate volume (PV) and free prostate-specific antigen (fPSA) and positively correlated with maximum urinary flow rate (Qmax). WNT-1 and β-catenin were positively related with International Prostate Symptom Score (IPSS) and nocturia, respectively. Our novel data demonstrate that SV could modulate cell proliferation, apoptosis, tissue fibrosis, and the EMT process in the prostate through crosstalk between PPARγ and WNT/β-catenin pathways.
VRK1 Kinase Activity Modulating Histone H4K16 Acetylation Inhibited by SIRT2 and VRK-IN-1
Authors: Monte-Serrano Eva , Lazo Pedro A.
Published: March 2023

The accessibility of DNA to different cellular functions requires a dynamic regulation of chromatin organization that is mediated by different epigenetic modifications, which regulate chromatin accessibility and degree of compaction. These epigenetic modifications, particularly the acetylation of histone H4 in lysine 14 (H4K16ac), determine the degree of chromatin accessibility to different nuclear functions, as well as to DNA damage drugs. H4K16ac is regulated by the balance between two alternative histone modifications, acetylation and deacetylation, which are mediated by acetylases and deacetylases. Tip60/KAT5 acetylates, and SIRT2 deacetylates histone H4K16. However, the balance between these two epigenetic enzymes is unknown. VRK1 regulates the level of H4K16 acetylation by activating Tip60. We have shown that the VRK1 and SIRT2 are able to form a stable protein complex. For this work, we used in vitro interaction, pull-down and in vitro kinase assays. In cells, their interaction and colocalization were detected by immunoprecipitation and immunofluorescence. The kinase activity of VRK1 is inhibited by a direct interaction of its N-terminal kinase domain with SIRT2 in vitro. This interaction causes a loss of H4K16ac similarly to the effect of a novel VRK1 inhibitor (VRK-IN-1) or VRK1 depletion. The use of specific SIRT2 inhibitors in lung adenocarcinoma cells induces H4K16ac, contrary to the novel VRK-IN-1 inhibitor, which prevents H4K16ac and a correct DNA damage response. Therefore, the inhibition of SIRT2 can cooperate with VRK1 in the accessibility of drugs to chromatin in response to DNA damage caused by doxorubicin.
A Mouse Systems Genetics Approach Reveals Common and Uncommon Genetic Modifiers of Hepatic Lysosomal Enzyme Activities and Glycosphingolipids
Authors: Durán Anyelo , Priestman David A. , Las Heras Macarena Las , Rebolledo-Jaramillo Boris , Olguín Valeria , Calderón Juan F. , Zanlungo Silvana , Gutiérrez Jaime , Platt Frances M. , Klein Andrés D.
Published: March 2023

Identification of genetic modulators of lysosomal enzyme activities and glycosphingolipids (GSLs) may facilitate the development of therapeutics for diseases in which they participate, including Lysosomal Storage Disorders (LSDs). To this end, we used a systems genetics approach: we measured 11 hepatic lysosomal enzymes and many of their natural substrates (GSLs), followed by modifier gene mapping by GWAS and transcriptomics associations in a panel of inbred strains. Unexpectedly, most GSLs showed no association between their levels and the enzyme activity that catabolizes them. Genomic mapping identified 30 shared predicted modifier genes between the enzymes and GSLs, which are clustered in three pathways and are associated with other diseases. Surprisingly, they are regulated by ten common transcription factors, and their majority by miRNA-340p. In conclusion, we have identified novel regulators of GSL metabolism, which may serve as therapeutic targets for LSDs and may suggest the involvement of GSL metabolism in other pathologies.
The CaSR Modulator NPS-2143 Reduced UV-Induced DNA Damage in Skh:hr1 Hairless Mice but Minimally Inhibited Skin Tumours
Authors: Yang Chen , Rybchyn Mark Stephen , De Silva Warusavithana Gunawardena Manori , Matthews Jim , Dixon Katie Marie , Holland Andrew J. A. , Conigrave Arthur David , Mason Rebecca Sara
Published: March 2023

The calcium-sensing receptor (CaSR) is an important regulator of epidermal function. We previously reported that knockdown of the CaSR or treatment with its negative allosteric modulator, NPS-2143, significantly reduced UV-induced DNA damage, a key factor in skin cancer development. We subsequently wanted to test whether topical NPS-2143 could also reduce UV-DNA damage, immune suppression, or skin tumour development in mice. In this study, topical application of NPS-2143 (228 or 2280 pmol/cm2) to Skh:hr1 female mice reduced UV-induced cyclobutane pyrimidine dimers (CPD) (p < 0.05) and oxidative DNA damage (8-OHdG) (p < 0.05) to a similar extent as the known photoprotective agent 1,25(OH)2 vitamin D3 (calcitriol, 1,25D). Topical NPS-2143 failed to rescue UV-induced immunosuppression in a contact hypersensitivity study. In a chronic UV photocarcinogenesis protocol, topical NPS-2143 reduced squamous cell carcinomas for only up to 24 weeks (p < 0.02) but had no other effect on skin tumour development. In human keratinocytes, 1,25D, which protected mice from UV-induced skin tumours, significantly reduced UV-upregulated p-CREB expression (p < 0.01), a potential early anti-tumour marker, while NPS-2143 had no effect. This result, together with the failure to reduce UV-induced immunosuppression, may explain why the reduction in UV-DNA damage in mice with NPS-2143 was not sufficient to inhibit skin tumour formation.