bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Antioxidant Methyl Derivatives of Ortho-Carbonyl Hydroquinones That Reduce Caco-2 Cell Energetic Metabolism and Alpha-Glucosidase Activity
Authors: Monroy-Cárdenas Matías , Almarza Cristopher , Valenzuela-Hormazábal Paulina , Ramírez David , Urra Félix A. , Martínez-Cifuentes Maximiliano , Araya-Maturana Ramiro
Published: July 2024

α-glucosidase, a pharmacological target for type 2 diabetes mellitus (T2DM), is present in the intestinal brush border membrane and catalyzes the hydrolysis of sugar linkages during carbohydrate digestion. Since α-glucosidase inhibitors (AGIs) modulate intestinal metabolism, they may influence oxidative stress and glycolysis inhibition, potentially addressing intestinal dysfunction associated with T2DM. Herein, we report on a study of an ortho-carbonyl substituted hydroquinone series, whose members differ only in the number and position of methyl groups on a common scaffold, on radical-scavenging activities (ORAC assay) and correlate them with some parameters obtained by density functional theory (DFT) analysis. These compounds’ effect on enzymatic activity, their molecular modeling on α-glucosidase, and their impact on the mitochondrial respiration and glycolysis of the intestinal Caco-2 cell line were evaluated. Three groups of compounds, according their effects on the Caco-2 cells metabolism, were characterized: group A (compounds 2, 3, 5, 8, 9, and 10) reduces the glycolysis, group B (compounds 1 and 6) reduces the basal mitochondrial oxygen consumption rate (OCR) and increases the extracellular acidification rate (ECAR), suggesting that it induces a metabolic remodeling toward glycolysis, and group C (compounds 4 and 7) increases the glycolysis lacking effect on OCR. Compounds 5 and 10 were more potent as α-glucosidase inhibitors (AGIs) than acarbose, a well-known AGI with clinical use. Moreover, compound 5 was an OCR/ECAR inhibitor, and compound 10 was a dual agent, increasing the proton leak-driven OCR and inhibiting the maximal electron transport flux. Additionally, menadione-induced ROS production was prevented by compound 5 in Caco-2 cells. These results reveal that slight structural variations in a hydroquinone scaffold led to diverse antioxidant capability, α-glucosidase inhibition, and the regulation of mitochondrial bioenergetics in Caco-2 cells, which may be useful in the design of new drugs for T2DM and metabolic syndrome.
Proteomic Analysis Reveals Physiological Activities of Aβ Peptide for Alzheimer’s Disease
Authors: Ai Xiaorui , Cao Zeyu , Ma Zhaoru , Liu Qinghuan , Huang Wei , Sun Taolei , Li Jing , Yang Chenxi
Published: July 2024

With the rapid progress in deciphering the pathogenesis of Alzheimer’s disease (AD), it has been widely accepted that the accumulation of misfolded amyloid β (Aβ) in the brain could cause the neurodegeneration in AD. Although much evidence demonstrates the neurotoxicity of Aβ, the role of Aβ in the nervous system are complex. However, more comprehensive studies are needed to understand the physiological effect of Aβ40 monomers in depth. To explore the physiological mechanism of Aβ, we employed mass spectrometry to investigate the altered proteomic events induced by a lower submicromolar concentration of Aβ. Human neuroblastoma SH-SY5Y cells were exposed to five different concentrations of Aβ1-40 monomers and collected at four time points. The proteomic analysis revealed the time–course behavior of proteins involved in biological processes, such as RNA splicing, nuclear transport and protein localization. Further biological studies indicated that Aβ40 monomers may activate PI3K/AKT signaling to regulate p-Tau, Ezrin and MAP2. These three proteins are associated with dendritic morphogenesis, neuronal polarity, synaptogenesis, axon establishment and axon elongation. Moreover, Aβ40 monomers may regulate their physiological forms by inhibiting the expression of BACE1 and APP via activation of the ERK1/2 pathway. A comprehensive exploration of pathological and physiological mechanisms of Aβ is beneficial for exploring novel treatment.
Establishment and Characterization of a Chicken Myoblast Cell Line
Authors: Guo Dongxue , Lin Shudai , Wang Xiaotong , Jiao Zhenhai , Li Guo , An Lilong , Zhang Zihao , Zhang Li
Published: July 2024

Skeletal muscle, which is predominantly constituted by multinucleated muscle fibers, plays a pivotal role in sustaining bodily movements and energy metabolism. Myoblasts, which serve as precursor cells for differentiation and fusion into muscle fibers, are of critical importance in the exploration of the functional genes associated with embryonic muscle development. However, the in vitro proliferation of primary myoblasts is inherently constrained. In this study, we achieved a significant breakthrough by successfully establishing a chicken myoblast cell line through the introduction of the exogenous chicken telomerase reverse transcriptase (chTERT) gene, followed by rigorous G418-mediated pressure screening. This newly developed cell line, which was designated as chTERT-myoblasts, closely resembled primary myoblasts in terms of morphology and exhibited remarkable stability in culture for at least 20 generations of population doublings without undergoing malignant transformation. In addition, we conducted an exhaustive analysis that encompassed cellular proliferation, differentiation, and transfection characteristics. Our findings revealed that the chTERT-myoblasts had the ability to proliferate, differentiate, and transfect after multiple rounds of population doublings. This achievement not only furnished a valuable source of homogeneous avian cell material for investigating embryonic muscle development, but also provided valuable insights and methodologies for establishing primary cell lines.
Characterization of GPX Gene Family in Pepper (Capsicum annuum L.) under Abiotic Stress and ABA Treatment
Authors: Zhang Zeyu , Zhang Jing , Wang Cheng , Chang Youlin , Han Kangning , Gao Yanqiang , Xie Jianming
Published: July 2024

Plant glutathione peroxidases (GPXs) are important enzymes for removing reactive oxygen species in plant cells and are closely related to the stress resistance of plants. This study identified the GPX gene family members of pepper (Capsicum annuum L.), “CM333”, at the whole-genome level to clarify their expression patterns and enzyme activity changes under abiotic stress and ABA treatment. The results showed that eight CaGPX genes were unevenly distributed across four chromosomes and one scaffold of the pepper genome, and their protein sequences had Cys residues typical of the plant GPX domains. The analysis of collinearity, phylogenetic tree, gene structure, and conserved motifs indicated that the CaGPX gene sequence is conserved, structurally similar, and more closely related to the sequence structure of Arabidopsis. Meanwhile, many cis elements involved in stress, hormones, development, and light response were found in the promoter region of the CaGPX gene. In addition, CaGPX1/4 and CaGPX6 were basically expressed in all tissues, and their expression levels were significantly upregulated under abiotic stress and ABA treatment. Subcellular localization showed that CaGPX1 and CaGPX4 are localized in chloroplasts. Additionally, the variations in glutathione peroxidase activity (GSH-Px) mostly agreed with the variations in gene expression. In summary, the CaGPXs gene may play an important role in the development of peppers and their response to abiotic stress and hormones.
Alcohol- and Low-Iron Induced Changes in Antioxidant and Energy Metabolism Associated with Protein Lys Acetylation
Authors: Thornton Jesse A. , Koc Zeynep C. , Sollars Vincent E. , Valentovic Monica A. , Denvir James , Wilkinson John , Koc Emine C.
Published: July 2024

Understanding the role of iron in ethanol-derived hepatic stress could help elucidate the efficacy of dietary or clinical interventions designed to minimize liver damage from chronic alcohol consumption. We hypothesized that normal levels of iron are involved in ethanol-derived liver damage and reduced dietary iron intake would lower the damage caused by ethanol. We used a pair-fed mouse model utilizing basal Lieber-DeCarli liquid diets for 22 weeks to test this hypothesis. In our mouse model, chronic ethanol exposure led to mild hepatic stress possibly characteristic of early-stage alcoholic liver disease, seen as increases in liver-to-body weight ratios. Dietary iron restriction caused a slight decrease in non-heme iron and ferritin (FeRL) expression while it increased transferrin receptor 1 (TfR1) expression without changing ferroportin 1 (FPN1) expression. It also elevated protein lysine acetylation to a more significant level than in ethanol-fed mice under normal dietary iron conditions. Interestingly, iron restriction led to an additional reduction in nicotinamide adenine dinucleotide (NAD+) and NADH levels. Consistent with this observation, the major mitochondrial NAD+-dependent deacetylase, NAD-dependent deacetylase sirtuin-3 (SIRT3), expression was significantly reduced causing increased protein lysine acetylation in ethanol-fed mice at normal and low-iron conditions. In addition, the detection of superoxide dismutase 1 and 2 levels (SOD1 and SOD2) and oxidative phosphorylation (OXPHOS) complex activities allowed us to evaluate the changes in antioxidant and energy metabolism regulated by ethanol consumption at normal and low-iron conditions. We observed that the ethanol-fed mice had mild liver damage associated with reduced energy and antioxidant metabolism. On the other hand, iron restriction may exacerbate certain activities of ethanol further, such as increased protein lysine acetylation and reduced antioxidant metabolism. This metabolic change may prove a barrier to the effectiveness of dietary reduction of iron intake as a preventative measure in chronic alcohol consumption.
Identification of Oxidative Stress-Related Biomarkers for Pain–Depression Comorbidity Based on Bioinformatics
Authors: Zhang Tianyun , Geng Menglu , Li Xiaoke , Gu Yulin , Zhao Wenjing , Ning Qi , Zhao Zijie , Wang Lei , Zhang Huaxing , Zhang Fan
Published: July 2024

Oxidative stress has been identified as a major factor in the development and progression of pain and psychiatric disorders, but the underlying biomarkers and molecular signaling pathways remain unclear. This study aims to identify oxidative stress-related biomarkers and signaling pathways in pain–depression comorbidity. Integrated bioinformatics analyses were applied to identify key genes by comparing pain–depression comorbidity-related genes and oxidative stress-related genes. A total of 580 differentially expressed genes and 35 differentially expressed oxidative stress-related genes (DEOSGs) were identified. By using a weighted gene co-expression network analysis and a protein–protein interaction network, 43 key genes and 5 hub genes were screened out, respectively. DEOSGs were enriched in biological processes and signaling pathways related to oxidative stress and inflammation. The five hub genes, RNF24, MGAM, FOS, and TKT, were deemed potential diagnostic and prognostic markers for patients with pain–depression comorbidity. These genes may serve as valuable targets for further research and may aid in the development of early diagnosis, prevention strategies, and pharmacotherapy tools for this particular patient population.
Paeoniflorin Promotes Ovarian Development in Mice by Activating Mitophagy and Preventing Oxidative Stress
Authors: Xi Huaming , Wang Ziqian , Li Minghui , Duan Xing , Li Yuan
Published: July 2024

During the development of animal organs, various adverse stimuli or toxic environments can induce oxidative stress and delay ovarian development. Paeoniflorin (PF), the main active ingredient of the traditional Chinese herb Paeonia lactiflora Pall., has protective effects on various diseases by preventing oxidative stress. However, the mechanism by which PF attenuates oxidative damage in mouse ovaries remains unclear. We evaluated the protective effects of PF on ovaries in an H2O2-induced mouse oxidative stress model. The H2O2-induced mouse ovarian oxidative stress model was used to explore the protective effect of PF on ovarian development. Histology and follicular development were observed. We then detected related indicators of cell apoptosis, oxidative stress, and autophagy in mouse ovaries. We found that PF inhibited H2O2-induced ovarian cell apoptosis and ferroptosis and promoted granulosa cell proliferation. PF prevented oxidative stress by increasing nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression levels. In addition, the autophagic flux of ovarian cells was activated and was accompanied by increased lysosomal biogenesis. Moreover, PF-mediated autophagy was involved in clearing mitochondria damaged by H2O2. Importantly, PF administration significantly increased the number of primordial follicles, primary follicles, secondary follicles, and antral follicles. PF administration improved ovarian sizes compared with the H2O2 group. The present study suggested that PF administration reversed H2O2-induced ovarian developmental delay and promoted follicle development. PF-activated mitophagy is crucial for preventing oxidative stress and improving mitochondrial quality.
Activity of N-Chlorotaurine against Periodontal Pathogens
Authors: Kowalczyk Kacper , Coraça-Huber Débora C. , Wille-Kollmar Walter , Berktold Michael , Nagl Markus
Published: July 2024

Dental plaque bacteria play an important role in the pathogenicity of periodontitis and peri-implantitis. Therefore, antimicrobial agents are one means of treatment. N-chlorotaurine (NCT) as an endogenous well-tolerated topical antiseptic could be of advantage for this purpose. Accordingly, its microbicidal activity against some dental plaque bacteria was investigated at therapeutic concentrations in vitro. In quantitative killing assays, the activity of NCT against planktonic bacteria and against biofilms grown for 48 h on implantation screws was tested. Electron microscopy was used to demonstrate the formation of biofilm and its morphological changes. The killing of planktonic bacteria of all tested species, namely Streptococcus sanguinis, Streptococcus salivarius, Streptococcus oralis, Streptococcus cristatus, Rothia aeria, and Capnocytophaga ochracea, was shown within 10–20 min by 1% NCT in 0.01 M phosphate-buffered saline at 37 °C. Bacteria grown on screws for 24 h were inactivated by 1% NCT after 15–20 min as well, but the formation of biofilm on the screws was visible in electron microscopy not before 48 h. The killing of biofilms by 1% NCT was demonstrated after 30 min (streptococci) and 40 min (R. aeria). As expected, NCT has broad activity against dental plaque bacteria as well and should be further investigated on its clinical efficacy in periodontitis and peri-implantitis.
MALDI Imaging Mass Spectrometry Reveals Lipid Alterations in Physiological and Sertoli Cell-Only Syndrome Human Testicular Tissue Sections
Authors: Sulc Alexandra , Czétány Péter , Máté Gábor , Balló András , Semjén Dávid , Szántó Árpád , Márk László
Published: July 2024

Azoospermia, the absence of sperm cells in semen, affects around 15% of infertile males. Sertoli cell-only syndrome (SCOS) is the most common pathological lesion in the background of non-obstructive azoospermia and is characterised by the complete absence of germinal epithelium, with Sertoli cells exclusively present in the seminiferous tubules. Studies have shown a correlation between successful spermatogenesis and male fertility with lipid composition of spermatozoa, semen, seminal plasma or testis. The aim of this research was to discover the correlation between the Johnsen scoring system and phospholipid expressions in testicular cryosections of SCOS patients. MALDI imaging mass spectrometry is used to determine spatial distributions of molecular species, such as phospholipids. Phosphatidylcholines (PCs), phosphatidylethanolamines (PEs) and sphingomyelins (SMs) are the most abundant phospholipids in mammalian cells and testis. SMs, the structural components of plasma membranes, are crucial for spermatogenesis and sperm function. Plasmalogens, are unique PCs in testis with strong antioxidative properties. This study, using imaging mass spectrometry, demonstrates the local distribution of phospholipids, particularly SMs, PCs, plasmalogens and PEs in human testicular samples with SCOS for the first time. This study found a strong relationship between the Johnsen scoring system and phospholipid expression levels in human testicular tissues. Future findings could enable routine diagnostic techniques during microTESE procedures for successful sperm extraction.
Physiological Features of the Neural Stem Cells Obtained from an Animal Model of Spinal Muscular Atrophy and Their Response to Antioxidant Curcumin
Authors: Adami Raffaella , Pezzotta Matteo , Cadile Francesca , Cuniolo Beatrice , Rovati Gianenrico , Canepari Monica , Bottai Daniele
Published: July 2024

The most prevalent rare genetic disease affecting young individuals is spinal muscular atrophy (SMA), which is caused by a loss-of-function mutation in the telomeric gene survival motor neuron (SMN) 1. The high heterogeneity of the SMA pathophysiology is determined by the number of copies of SMN2, a separate centromeric gene that can transcribe for the same protein, although it is expressed at a slower rate. SMA affects motor neurons. However, a variety of different tissues and organs may also be affected depending on the severity of the condition. Novel pharmacological treatments, such as Spinraza, Onasemnogene abeparvovec-xioi, and Evrysdi, are considered to be disease modifiers because their use can change the phenotypes of the patients. Since oxidative stress has been reported in SMA-affected cells, we studied the impact of antioxidant therapy on neural stem cells (NSCs) that have the potential to differentiate into motor neurons. Antioxidants can act through various pathways; for example, some of them exert their function through nuclear factor (erythroid-derived 2)-like 2 (NRF2). We found that curcumin is able to induce positive effects in healthy and SMA-affected NSCs by activating the nuclear translocation of NRF2, which may use a different mechanism than canonical redox regulation through the antioxidant-response elements and the production of antioxidant molecules.
Research Progress on the Role of M6A in Regulating Economic Traits in Livestock
Authors: Ren Tuanhui , Xu Meng , Du Xinyu , Wang Yanxi , Loor Juan J. , Lei Lin , Gao Wenwen , Du Xiliang , Song Yuxiang , Liu Guowen , Li Xinwei
Published: July 2024

Reversible regulation of N6-methyladenosine (m6A) methylation of eukaryotic RNA via methyltransferases is an important epigenetic event affecting RNA metabolism. As such, m6A methylation plays crucial roles in regulating animal growth, development, reproduction, and disease progression. Herein, we review the latest research advancements in m6A methylation modifications and discuss regulatory aspects in the context of growth, development, and reproductive traits of livestock. New insights are highlighted and perspectives for the study of m6A methylation modifications in shaping economically important traits are discussed.
Influence of Post-Weld Heat Treatment on Mechanical Properties and Microstructure of Plasma Arc-Welded 316 Stainless Steel
Authors: Baisukhan Adirek , Naksuk Nirut , Insua Pinmanee , Nakkiew Wasawat , Wisittipanit Nuttachat
Published: July 2024

This study investigates the effects of post-weld heat treatment (PWHT) on the microstructures and mechanical properties of plasma arc-welded 316 stainless steel. The experimental parameters included the solid solution temperatures of 650 °C and 1050 °C, solid solution durations of 1 h and 4 h, and quenching media of water and air. The mechanical properties were evaluated using Vickers hardness testing, tensile testing, scanning electron microscopy (SEM), and optical microscopy (OM). The highest ultimate tensile strength (UTS) of 693.93 MPa and Vickers hardness of 196.4 in the welded zone were achieved by heat-treating at 650 °C for one hour, quenching in water, and aging at 500 °C for 24 h. Heat-treating at 650 °C for one hour, followed by quenching in water and aging at 500 °C for 24 h results in larger dendritic δ grains and contains more σ phase compared to the other conditions, resulting in increased strength and hardness. Additionally, it shows wider and shallower dimple structures, which account for its reduced impact toughness.
Nebulized Glutathione as a Key Antioxidant for the Treatment of Oxidative Stress in Neurodegenerative Conditions
Authors: Lana João Vitor , Rios Alexandre , Takeyama Renata , Santos Napoliane , Pires Luyddy , Santos Gabriel Silva , Rodrigues Izair Jefthé , Jeyaraman Madhan , Purita Joseph , Lana Jose Fábio
Published: July 2024

Glutathione (GSH), a tripeptide synthesized intracellularly, serves as a pivotal antioxidant, neutralizing reactive oxygen species (ROS) and reactive nitrogen species (RNS) while maintaining redox homeostasis and detoxifying xenobiotics. Its potent antioxidant properties, particularly attributed to the sulfhydryl group (-SH) in cysteine, are crucial for cellular health across various organelles. The glutathione-glutathione disulfide (GSH-GSSG) cycle is facilitated by enzymes like glutathione peroxidase (GPx) and glutathione reductase (GR), thus aiding in detoxification processes and mitigating oxidative damage and inflammation. Mitochondria, being primary sources of reactive oxygen species, benefit significantly from GSH, which regulates metal homeostasis and supports autophagy, apoptosis, and ferroptosis, playing a fundamental role in neuroprotection. The vulnerability of the brain to oxidative stress underscores the importance of GSH in neurological disorders and regenerative medicine. Nebulization of glutathione presents a novel and promising approach to delivering this antioxidant directly to the central nervous system (CNS), potentially enhancing its bioavailability and therapeutic efficacy. This method may offer significant advantages in mitigating neurodegeneration by enhancing nuclear factor erythroid 2-related factor 2 (NRF2) pathway signaling and mitochondrial function, thereby providing direct neuroprotection. By addressing oxidative stress and its detrimental effects on neuronal health, nebulized GSH could play a crucial role in managing and potentially ameliorating conditions such as Parkinson’s Disease (PD) and Alzheimer’s Disease (AD). Further clinical research is warranted to elucidate the therapeutic potential of nebulized GSH in preserving mitochondrial health, enhancing CNS function, and combating neurodegenerative conditions, aiming to improve outcomes for individuals affected by brain diseases characterized by oxidative stress and neuroinflammation.
Ashwagandha’s Multifaceted Effects on Human Health: Impact on Vascular Endothelium, Inflammation, Lipid Metabolism, and Cardiovascular Outcomes—A Review
Authors: Wiciński Michał , Fajkiel-Madajczyk Anna , Kurant Zuzanna , Liss Sara , Szyperski Paweł , Szambelan Monika , Gromadzki Bartłomiej , Rupniak Iga , Słupski Maciej , Sadowska-Krawczenko Iwona
Published: July 2024

Withania somnifera, commonly known as Ashwagandha, has been popular for many years. Numerous studies have shown that the extract of this plant, due to its wealth of active substances, can induce anti-inflammatory, neuroprotective, immunomodulatory, hepatoprotective, cardioprotective, anti-diabetic, adaptogenic, anti-arthritic, anti-stress, and antimicrobial effects. This review examines the impact of Ashwagandha extract on the vascular endothelium, inflammation, lipid metabolism, and cardiovascular outcomes. Studies have shown that Ashwagandha extracts exhibit an anti-angiogenic effect by inhibiting vascular endothelial growth factor (VEGF)-induced capillary sprouting and formation by lowering the mean density of microvessels. Furthermore, the results of numerous studies highlight the anti-inflammatory role of Ashwagandha extract, as the action of this plant causes a decrease in the expression of pro-inflammatory cytokines. Interestingly, withanolides, present in Ashwagandha root, have shown the ability to inhibit the differentiation of preadipocytes into adipocytes. Research results have also proved that W. somnifera demonstrates cardioprotective effects due to its antioxidant properties and reduces ischemia/reperfusion-induced apoptosis. It seems that this plant can be successfully used as a potential treatment for several conditions, mainly those with increased inflammation. More research is needed to elucidate the exact mechanisms by which the substances contained in W. somnifera extracts can act in the human body.
Adherence to the Mediterranean Diet Mitigates Inflammation and Hospital Stay in Frail Elderly Patients: A Moderation Analysis
Authors: Lo Buglio Aurelio , Bellanti Francesco , Carapellese Rosanna Maria , Capurso Cristiano , Serviddio Gaetano , Vendemiale Gianluigi
Published: July 2024

Understanding the interaction between dietary patterns and nutritional status in influencing health outcomes is crucial, especially in vulnerable populations. Our study investigates the impact of adherence to the Mediterranean diet (MD) and nutritional status on inflammatory markers (CRP) and the length of stay (LOS) in hospitalized frail elderly patients. Methods: We conducted two-way ANOVA and multiple regression analysis to evaluate the effects of nutritional status and MD adherence on the CRP levels and LOS in a cohort of 117 frail elderly patients aged 65 years or older. Patients with cancer or acute infection were excluded. Adherence to the MD was assessed using the 14-item PREDIMED questionnaire. Results: Significant interactions were found between nutritional status and MD adherence for both the CRP and LOS. The patients with low-level MD adherence and a poor nutritional status exhibited higher CRP levels and longer hospital stays compared to those with high MD adherence. Specifically, a statistically significant interaction was observed for the CRP (F (1, 113) = 7.36, p = 0.008) and LOS (F (1, 113) = 15.4, p < 0.001), indicating the protective effect of high-level MD adherence. Moderation analysis confirmed that high-level MD adherence mitigates the adverse effects of malnutrition on both the inflammatory response and LOS. Conclusions: These findings highlight the importance of promoting the MD, particularly in malnourished elderly patients, to improve health outcomes and reduce hospitalization duration. Further longitudinal studies are warranted to establish causality and explore the underlying mechanisms.
Analysis of Sports Supplement Consumption of Elite Referees of the Spanish Professional Fotball League
Authors: Sánchez-Oliver Antonio Jesús , Moreno-Pérez Víctor , Terrón-Manrique Pablo , Fernández-Ruiz Vicente , Quintana-Milla Iñaki , Sánchez-Sánchez Javier , Rodríguez Guillermo , Ramos-Álvarez Juan José , Domínguez Raúl , López-Samanes Álvaro
Published: July 2024

Background: Sports supplements (SSs) are widely used among team sport athletes; however, evidence supporting the use of SSs among football referees at the elite level is scarce. The aim of the present study was to analyze the consumption of SSs among referees with respect to their level of competition and referee type (main referees (MRs) vs. assistant referees (ARs)). Methods: A total of 106 football referees participated in this study, with 46.2% from the First Spanish Division and 53.8% from the Second Spanish Division, representing 84.13% of the total number of referees. Each participant completed a validated questionnaire about SS consumption, with the SSs classified according to the Australian Institute of Sport (AIS) ABCD system: Group A has strong evidence for enhancing athlete health and performance, Group B shows potential benefits but needs more evidence, Group C has inconclusive evidence against use, and Group D includes prohibited substances. Results: A total of 84.0% of the MRs and ARs reported the consumption of at least one SS. Differences were found only in the consumption of medical supplements between division (p = 0.016) and type of referee (p = 0.041), though no significant differences were found among the remaining AIS SS categories (p > 0.05). Sport performance (49.6%), Internet (41.0%), and dietitian–nutritionists (31.7%) were the primary reason for SS consumption, purchase location, and source of information, respectively. The most frequently consumed SS were whey protein (45.3%), followed by creatine (33.0%), sport bars and sports drinks (28.3%), and caffeine (19.8%). Conclusions: MRs and ARs reported a high prevalence of dietary supplement (SS) consumption, with significant differences between division and referee type observed only in medical supplement consumption.
Body Mass Index, Adherence to a Healthy Lifestyle, and Breakfast Consumption Associated with Religious Affiliation in Peruvian University Students: A Cross-Sectional Study
Authors: Lévano-Matos Luis , Saintila Jacksaint , Gálvez-Díaz Norma Del Carmen , Calizaya-Milla Yaquelin E.
Published: July 2024

Background: Despite evidence suggesting a relationship between religiosity and health habits, there is a paucity of studies specifically examining this association in the context of Peruvian university students. This study compared body mass index (BMI), adherence to a healthy lifestyle, and breakfast consumption in Peruvian university students of four religious denominations: Seventh Day Adventists (SDA), Catholics, Baptists, and Evangelicals. Methods: A cross-sectional study was conducted online among 4557 students from a Peruvian university. The BMI and the frequency of breakfast consumption were evaluated, and the Diet and Healthy Lifestyle Scale (DEVS) was applied. The variables studied were associated using simple and multiple linear regression and Poisson models with robust variance. Results: Baptist (B = 0.44, 95% CI: 0.10–0.78; p = 0.011), Catholic (B = 0.3, 95% CI: 0.12–0.47; p = 0.001), and Evangelical (B = 0.32, 95% CI: 0.09 to 0.64; p = 0.014) students had a significantly higher BMI compared to SDA. Baptist (B = −0.2, 95% CI: −0.37–−0.05; p = 0.017) and Evangelical (B = −0.13, 95% CI: −0.28–−0.03; p = 0.012) students exhibited a lower mean score on the measure of healthy lifestyles compared to SDA students. Additionally, Baptist (PR = −0.32, 95% CI: −0.92–−0.12; p = 0.035) and Catholic (PR = −0.3, 95% CI: −0.99–−0.19; p = 0.016) students exhibited a lower probability of eating breakfast regularly compared to SDA students. Conclusions: Health professionals should consider these findings when designing and implementing health promotion programs that are culturally sensitive and respectful of the beliefs and practices of all religious groups in university settings.
Food Insecurity Associated with Higher Stress, Depressive Symptoms, and Lower Diet Quality among Women Caregivers in North Carolina
Authors: Butler J. Lauren , Johnson Cassandra M. , Hardison-Moody Annie , Bowen Sarah K.
Published: July 2024

Background: Research suggests a bidirectional relationship between food insecurity and stress, but few studies have examined associations of food insecurity with stress and other indicators of cardiometabolic health, including depression, diet quality, and body weight, among lower-income women in the U.S. Methods: This cross-sectional study analyzed data from lower-income women caregivers living in North Carolina (n = 100): 42% Black/African American, 25% Hispanic/Latina, and 33% White women. Multivariable linear regression models were used to determine associations of food insecurity status with perceived stress, depressive symptoms, diet quality, and body mass index (BMI). Multivariable logistic regression models were used to determine associations of food insecurity with clinical depression and BMI ≥ 30 kg/m2. Associations were examined with and without adjustment for perceived stress. Results: Forty-two percent of the sample were experiencing food insecurity. Compared to food secure caregivers, food-insecure caregivers had significantly higher perceived stress (β: +7.51; 95%CI: 4.19, 10.84) and depressive symptoms (β: +3.55; 95%CI: 0.54, 6.56) and lower diet quality (β: −9.10; 95%CI: −15.81, −2.40). Associations with BMI outcomes were not statistically significant. Conclusion: Findings support removing stigma in nutrition assistance programs and clinical interactions, motivate future longitudinal studies, and inform the development of destigmatizing interventions for health promotion or disease prevention.
The Role of Nutrition on Thyroid Function
Authors: Shulhai Anna-Mariia , Rotondo Roberta , Petraroli Maddalena , Patianna Viviana , Predieri Barbara , Iughetti Lorenzo , Esposito Susanna , Street Maria Elisabeth
Published: July 2024

Thyroid function is closely linked to nutrition through the diet–gut–thyroid axis. This narrative review highlights the influence of nutritional components and micronutrients on thyroid development and function, as well as on the gut microbiota. Micronutrients such as iodine, selenium, iron, zinc, copper, magnesium, vitamin A, and vitamin B12 influence thyroid hormone synthesis and regulation throughout life. Dietary changes can alter the gut microbiota, leading not just to dysbiosis and micronutrient deficiency but also to changes in thyroid function through immunological regulation, nutrient absorption, and epigenetic changes. Nutritional imbalance can lead to thyroid dysfunction and/or disorders, such as hypothyroidism and hyperthyroidism, and possibly contribute to autoimmune thyroid diseases and thyroid cancer, yet controversial issues. Understanding these relationships is important to rationalize a balanced diet rich in essential micronutrients for maintaining thyroid health and preventing thyroid-related diseases. The synthetic comprehensive overview of current knowledge shows the importance of micronutrients and gut microbiota for thyroid function and uncovers potential gaps that require further investigation.
Long Non-Coding H19 in Lymphocytes: Prognostic Value in Acute Ischemic Stroke Patients
Authors: Zhong Liyuan , Xie Zixian , Han Ziping , Fan Junfen , Wang Rongliang , Tao Zhen , Ma Qingfeng , Luo Yumin
Published: July 2024

Acute ischemic stroke (AIS) is a cerebrovascular disease that seriously affects the physical and mental health and quality of life of patients. However, there is a lack of reliable prognostic prediction methods. The main objective of this study was to investigate the prognostic value of long non-coding RNA (lncRNA) H19 in lymphocytes of patients with AIS, and to construct a prognostic prediction model for AIS including lncRNA H19 in lymphocytes, which would provide new ideas for the prognostic evaluation of AIS. Poor prognosis was defined when the patient’s modified Rankin scale (mRS) score at 3 months after AIS onset was greater than 2. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the level of lncRNA H19 in lymphocytes. Spearman correlation analysis revealed a positive correlation between lncRNA H19 and mRS score at 3 months after AIS onset (r = 0.1977, p = 0.0032), while lncRNA H19 was negatively correlated with white blood cells counts, lymphocytes counts, and neutrophils counts. Logistic regression analysis identified lncRNA H19 as an independent predictor of poor prognosis (OR = 3.062 [1.69–5.548], p < 0.001). Moreover, a nomogram prediction model incorporating lncRNA H19 in lymphocytes demonstrated effective discrimination, calibration, and clinical applicability in predicting AIS outcomes. The findings suggest that lncRNA H19 in lymphocytes could be a valuable prognostic indicator and a potential pharmacological target for AIS patients, and might be a novel pathway for enhanced prognostic evaluation and targeted therapeutic strategies.
Modeling Spatio-Temporal Rainfall Distribution in Beni–Irumu, Democratic Republic of Congo: Insights from CHIRPS and CMIP6 under the SSP5-8.5 Scenario
Authors: Posite Vithundwa Richard , Saber Mohamed , Ahana Bayongwa Samuel , Abdelbaki Cherifa , Bessah Enoch , Appiagyei Bright Danso , Maouly Djessy Karl , Danquah Jones Abrefa
Published: July 2024

In light of the lack of ground-based observations, this study utilizes reanalysis data from the CHIRPS database and CMIP6 models under the SSP5-8.5 scenario to predict future rainfall in the Beni–Irumu region of eastern DR Congo. The use of reanalysis data offers a viable method for understanding historical and future climate trends in regions with limited ground data. Using a spatial resolution of 0.05°, selected general circulation models (GCMs) were downscaled to CHIRPS data. Analysis of historical rainfall data over 32 years reveals spatial disparities, with high-altitude regions like Mount Stanley experiencing higher annual mean rainfall (1767.87 ± 174.41 mm) compared to lower areas like Kasenyi (863.65 ± 81.85 mm), in line with orographic effects. Future projections under the SSP5-8.5 scenario indicate significant decreases in rainfall for areas such as Oicha (−565.55 mm) in the near term, while regions like Kasindi/Yihunga exhibit moderate decrease (−58.5 mm). In the mid-term, some areas show signs of recovery, with Bulongo experiencing a minor decrease (−21.67 mm), and Kasindi/Yihunga (+152.95 mm) and Kyavinyonge (+71.11 mm) showing increases. Long-term projections suggest overall improvements, with most areas experiencing positive rainfall differences; however, persistent challenges remain in Oicha (−313.82 mm). These findings highlight the dynamic impacts of climate change on rainfall distribution in the Beni–Irumu region, underscoring the need for targeted interventions to address the varied impacts, especially in vulnerable regions like Oicha.
Muscle Network Connectivity Study in Diabetic Peripheral Neuropathy Patients
Authors: Junquera-Godoy Isabel , Martinez-De-Juan José Luís , González-Lorente Gemma , Carot-Sierra José Miguel , Gomis-Tena Julio , Saiz Javier , García-Blasco Silvia , Pertusa-Mazón Isabel , Soler-Climent Esther , Prats-Boluda Gema
Published: July 2024

Diabetic peripheral neuropathy (DPN) is a prevalent complication of chronic diabetes mellitus and has a significant impact on quality of life. DPN typically manifests itself as a symmetrical, length-dependent sensorimotor polyneuropathy with severe effects on gait. Surface electromyography (sEMG) is a valuable low-cost tool for assessing muscle activation patterns and precise identification of abnormalities. For the present study, we used information theory methods, such as cross-correlation (CC), normalized mutual information (NMI), conditional granger causality (CG-Causality), and transfer entropy (TE), to evaluate muscle network connectivity in three population groups: 33 controls (healthy volunteers, CT), 10 diabetic patients with a low risk of DPN (LW), and 17 moderate/high risk patients (MH). The results obtained indicated significant alterations in the intermuscular coupling mechanisms due to diabetes and DPN, with the TE group showing the best performance in detecting differences. The data revealed a significant increase in information transfer and muscle connectivity in the LW group over the CT group, while the MH group obtained significantly lower values for these metrics than the other two groups. These findings highlight the sEMG coupling metrics’ potential to reveal neuromuscular mechanisms that could aid the development of targeted rehabilitation strategies and help monitor DPN patients.
Salinity Measurement with a Plasmonic Sensor Based on Doubly Deposited Tapered Optical Fibers
Authors: Navarrete María-Cruz , Díaz-Herrera Natalia , González-Cano Agustín
Published: July 2024

Salinity is a very important parameter from an environmental perspective, and therefore, efficient and accurate systems are required for marine environmental monitoring and productive industries. A plasmonic sensor based on doubly deposited tapered optical fibers (DLUWTs—double-layer uniform-waist tapers) for the measurement of salinity is presented. The physical principle of the sensor, as well as its structure, is discussed, and its performance is experimentally demonstrated, obtaining very good sensitivities. The possibility of shifting towards higher wavelength measuring ranges associated with DLUWTs is also exploited. At the same time, we have considered the necessity of an extensive characterization of the behavior of the refractive index of salty water, both with variations in temperature and the composition of the salts dissolved. This is important due to the somehow changing reality of salinity measurements and the possibility of establishing new approaches for the determination of absolute salinity as opposed to practical salinity based on electrical conductivity measurements. The results obtained, which show high sensitivity and a good performance in general without the need for the use of semi-empirical algorithms, permit, in our opinion, an advance in the tendency towards refractometric determination of salinity with optical sensors apt for in situ, real-time, accurate measurements in realistic measuring conditions.
Controlled Porosity of Selective Laser Melting-Produced Thermal Pipes: Experimental Analysis and Machine Learning Approach for Pore Recognition on Pipes Surfaces
Authors: Malashin Ivan , Martysyuk Dmitry , Tynchenko Vadim , Nelyub Vladimir , Borodulin Aleksei , Gantimurov Andrei , Nisan Anton , Novozhilov Nikolay , Zelentsov Viatcheslav , Filimonov Aleksey , Galinovsky Andrey
Published: July 2024

This study investigates the methods for controlling porosity in thermal pipes manufactured using selective laser melting (SLM) technology. Experiments conducted include water permeability tests and surface roughness measurements, which are complemented by SEM image ML-based analysis for pore recognition. The results elucidate the impact of SLM printing parameters on water permeability. Specifically, an increase in hatch and point distances leads to a linear rise in permeability, while higher laser power diminishes permeability. Using machine learning (ML) techniques, precise pore identification on SEM images depicting surface microstructures of the samples is achieved. The average percentage of the surface area containing detected pores for microstructure samples printed with laser parameters (laser power (W) _ hatch distance (µm) _ point distance (µm)) 175_ 80_80 was found to be 5.2%, while for 225_120_120, it was 4.2%, and for 275_160_160, it was 3.8%. Pore recognition was conducted using the Haar feature-based method, and the optimal patch size was determined to be 36 pixels on monochrome images of microstructures with a magnification of 33×, which were acquired using a Leica S9 D microscope.
LiDAR-Based Urban Three-Dimensional Rail Area Extraction for Improved Train Collision Warnings
Authors: Shen Tuo , Zhou Jinhuang , Yuan Tengfei , Xie Yuanxiang , Zhang Xuanxiong
Published: July 2024

The intrusion of objects into track areas is a significant issue affecting the safety of urban rail transit systems. In recent years, obstacle detection technology based on LiDAR has been developed to identify potential issues, in which accurately extracting the track area is critical for segmentation and collision avoidance. However, because of the sparsity limitations inherent in LiDAR data, existing methods can only segment track regions over short distances, which are often insufficient given the speed and braking distance of urban rail trains. As such, a new approach is developed in this study to indirectly extract track areas by detecting references parallel to the rails (e.g., tunnel walls, protective walls, and sound barriers). Reference point selection and curve fitting are then applied to generate a reference curve on either side of the track. A centerline is then extrapolated from the two curves and expanded to produce a 2D track area with the given size specifications. Finally, the 3D track area is acquired by detecting the ground and removing points that are either too high or too low. The proposed technique was evaluated using a variety of scenes, including tunnels, elevated sections, and level urban rail transit lines. The results showed this method could successfully extract track regions from LiDAR data over significantly longer distances than conventional algorithms.
Accurate UAV Small Object Detection Based on HRFPN and EfficentVMamba
Authors: Wu Shixiao , Lu Xingyuan , Guo Chengcheng , Guo Hong
Published: July 2024

(1) Background: Small objects in Unmanned Aerial Vehicle (UAV) images are often scattered throughout various regions of the image, such as the corners, and may be blocked by larger objects, as well as susceptible to image noise. Moreover, due to their small size, these objects occupy a limited area in the image, resulting in a scarcity of effective features for detection. (2) Methods: To address the detection of small objects in UAV imagery, we introduce a novel algorithm called High-Resolution Feature Pyramid Network Mamba-Based YOLO (HRMamba-YOLO). This algorithm leverages the strengths of a High-Resolution Network (HRNet), EfficientVMamba, and YOLOv8, integrating a Double Spatial Pyramid Pooling (Double SPP) module, an Efficient Mamba Module (EMM), and a Fusion Mamba Module (FMM) to enhance feature extraction and capture contextual information. Additionally, a new Multi-Scale Feature Fusion Network, High-Resolution Feature Pyramid Network (HRFPN), and FMM improved feature interactions and enhanced the performance of small object detection. (3) Results: For the VisDroneDET dataset, the proposed algorithm achieved a 4.4% higher Mean Average Precision (mAP) compared to YOLOv8-m. The experimental results showed that HRMamba achieved a mAP of 37.1%, surpassing YOLOv8-m by 3.8% (Dota1.5 dataset). For the UCAS_AOD dataset and the DIOR dataset, our model had a mAP 1.5% and 0.3% higher than the YOLOv8-m model, respectively. To be fair, all the models were trained without a pre-trained model. (4) Conclusions: This study not only highlights the exceptional performance and efficiency of HRMamba-YOLO in small object detection tasks but also provides innovative solutions and valuable insights for future research.
A Novel Design of a Torsional Shape Memory Alloy Actuator for Active Rudder
Authors: Lima Felipe S. , Souto Cícero R. , Oliveira Andersson G. , Silvestre Alysson D. , Alves Railson M. N. , Santos Sebastião E. S. , Gomez Ricardo S. , Brito Glauco R. F. , Bezerra André L. D. , Santana Diogenes S. M. , Lima Antonio G. B.
Published: July 2024

SMA actuators are a group of lightweight actuators that offer advantages over conventional technology and allow for simple and compact solutions to the increasing demand for electrical actuation. In particular, an increasing number of SMA torsional actuator applications have been published recently due to their ability to supply rotational motion under load, resulting in advantages such as module simplification and the reduction of overall product weight. This paper presents the conceptual design, operating principle, experimental characterization and working performance of torsional actuators applicable in active rudder in aeronautics. The proposed application comprises a pair of SMA torsion springs, which bi-directionally actuate the actuator by Joule heating and natural cooling. The experimental results confirm the functionality of the torsion springs actuated device and show the rotation angle of the developed active rudder was about 30° at a heating current of 5 A. After the design and experiment, one of their chief drawbacks is their relatively slow operating speed in rudder positioning, but this can be improved by control strategy and small modifications to the actuator mechanism described in this work.