bluuebunny/crossref_metadata_2025 huggingface dataset:
Extracellular Release of Mitochondrial DNA: Triggered by Cigarette Smoke and Detected in COPD
Authors: Giordano Luca , Gregory Alyssa D. , Pérez Verdaguer Mireia , Ware Sarah A. , Harvey Hayley , DeVallance Evan , Brzoska Tomasz , Sundd Prithu , Zhang Yingze , Sciurba Frank C. , Shapiro Steven D. , Kaufman Brett A.
Published: January 2022

Cigarette smoke (CS) is the most common risk factor for chronic obstructive pulmonary disease (COPD). The present study aimed to elucidate whether mtDNA is released upon CS exposure and is detected in the plasma of former smokers affected by COPD as a possible consequence of airway damage. We measured cell-free mtDNA (cf-mtDNA) and nuclear DNA (cf-nDNA) in COPD patient plasma and mouse serum with CS-induced emphysema. The plasma of patients with COPD and serum of mice with CS-induced emphysema showed increased cf-mtDNA levels. In cell culture, exposure to a sublethal dose of CSE decreased mitochondrial membrane potential, increased oxidative stress, dysregulated mitochondrial dynamics, and triggered mtDNA release in extracellular vesicles (EVs). Mitochondrial DNA release into EVs occurred concomitantly with increased expression of markers that associate with DNA damage responses, including DNase III, DNA-sensing receptors (cGAS and NLRP3), proinflammatory cytokines (IL-1β, IL-6, IL-8, IL-18, and CXCL2), and markers of senescence (p16 and p21); the majority of the responses are also triggered by cytosolic DNA delivery in vitro. Exposure to a lethal CSE dose preferentially induced mtDNA and nDNA release in the cell debris. Collectively, the results of this study associate markers of mitochondrial stress, inflammation, and senescence with mtDNA release induced by CSE exposure. Because high cf-mtDNA is detected in the plasma of COPD patients and serum of mice with emphysema, our findings support the future study of cf-mtDNA as a marker of mitochondrial stress in response to CS exposure and COPD pathology.
From Drosophila to Human: Biological Function of E3 Ligase Godzilla and Its Role in Disease
Authors: Cabana Valérie C. , Lussier Marc P.
Published: January 2022

The ubiquitin–proteasome system is of fundamental importance in all fields of biology due to its impact on proteostasis and in regulating cellular processes. Ubiquitination, a type of protein post-translational modification, involves complex enzymatic machinery, such as E3 ubiquitin ligases. The E3 ligases regulate the covalent attachment of ubiquitin to a target protein and are involved in various cellular mechanisms, including the cell cycle, cell division, endoplasmic reticulum stress, and neurotransmission. Because the E3 ligases regulate so many physiological events, they are also associated with pathologic conditions, such as cancer, neurological disorders, and immune-related diseases. This review focuses specifically on the protease-associated transmembrane-containing the Really Interesting New Gene (RING) subset of E3 ligases. We describe the structure, partners, and physiological functions of the Drosophila Godzilla E3 ligase and its human homologues, RNF13, RNF167, and ZNRF4. Also, we summarize the information that has emerged during the last decade regarding the association of these E3 ligases with pathophysiological conditions, such as cancer, asthma, and rare genetic disorders. We conclude by highlighting the limitations of the current knowledge and pinpointing the unresolved questions relevant to RNF13, RNF167, and ZNRF4 ubiquitin ligases.
Characterization of Quasi-Static/Dynamic Contact Mechanical Properties of Mo Surface-Modified TC4
Authors: Gao Jie , Zheng Ke , Yu Shengwang , Hei Hongjun , Wu Yanxia , Gong Huarong , Ma Yong
Published: January 2022

By using the double glow plasma surface alloying technique, a Mo surface-modified layer is prepared on Ti6Al4V(TC4). The element concentration and microstructure are characterized with a glow-discharge optical emission spectroscope and a scanning electron microscope. The results indicate that the Mo modified layer is compact in structure and gradation in composition, which consists of a pure Mo deposition layer and a thick Mo diffusion layer. Nanoindentation test results indicate that the surface hardness of TC4 is significantly improved after surface modification. Therefore, the initial part of the Mo diffusion layer has higher hardness than the Mo deposition layer. The impact tests for 10,000 cycles at different loads demonstrate that impact load 100 N only causes small plastic deformation, while impact loads 300 N and 500 N could result in cracks. Combining nanoindentation test with finite element reverse analysis, plastic parameters of the Mo modified layer are quantitatively determined. By using the impact test and finite element forward analysis, the dynamic contact mechanical properties of Mo surface-modified TC4 are characterized. When the impact cycles are fixed, ring cracks firstly occur and then radial cracks occur with the impact load increase on the graded layer. The ring cracks are mainly caused by impact stretch fatigue and corresponding cyclic stress is between 3.53 and 2.62 GPa. The radial cracks are mainly related to the tension-compression fatigue and corresponding cyclic stress is between 3.92 and −0.97 GPa.
A New Design for Magnetic Poly(vinyl pivalate) for Biomedical Applications: Synthesis, Characterization, and Evaluation of Cytotoxicity in Fibroblasts, Keratinocytes, and Human Melanoma Cells
Authors: Araujo Robson T. , Neta Maria S. B. , Coaquira José A. H. , Chaves Sacha B. , Machado Fabricio
Published: January 2022

Polymers containing magnetic properties play an important role in biomedical therapies, such as embolotherapy or hyperthermia, for their differentiated properties. In this work, magnetite (Fe3O4) nanoparticles were synthesized by the coprecipitation method and dispersed into a thermoplastic matrix of poly(vinyl pivalate) through an emulsion polymerization process. The main goal was the individual encapsulation of magnetite nanoparticles to improve the magnetic response of the magneto-polymeric materials using polymerizable carboxylic acids as coating agents, minimizing the leaching of nanoparticles throughout the nanocomposite formation. For this purpose, synthesized magnetite had its surface modified by acrylic acid or methacrylic acid to improve its individual encapsulation during the polymerization step, thus generating a series of magnetic nanocomposite materials containing different amounts of magnetite intended for biomedical applications. X-ray diffractometry and TEM measurements provided a mean size of approximately 8 nm for the pure magnetite nanoparticles and a spherical morphology. Acid-functionalized Fe3O4 had a size of approximately 6 nm, while the nanocomposites showed a size of approximately 7 nm. Magnetization measurement provided a saturation magnetization value of approximately 75 emu/g and confirmed superparamagnetic behavior at room temperature. DSC analysis showed a glass transition temperature of 65 °C for poly(vinyl pivalate)-based nanocomposites. The tests realized with homopolymer and magnetic composites against different cell lineages (i.e., fibroblasts, keratinocytes, and human melanoma) to evaluate the levels of cytotoxicity showed good results in the different exposure times and concentrations used, since the obtained results showed cell viability greater than 70% compared to the control group, suggesting that the synthesized materials are very promising for medical applications.
Parametric Optimization of Local Resonant Sonic Crystals Window on Noise Attenuation by Using Taguchi Method and ANOVA Analysis
Authors: Lee Hsiao Mun , Hua Yuting , Xie Jinlong , Lee Heow Pueh
Published: January 2022

Local resonant sonic crystals (LRSCs) window as a novel design has recently been proposed to achieve a good balance between noise mitigation, natural ventilation and natural lighting. In an effort to explore the feasibilities of such designs in civil residential buildings, an optimization methodology was proposed to develop a more compact LRSCs window with high noise attenuation performance in the present study. Specifically, the Taguchi method was adopted for the design of experiments on the parameters of interest and their corresponding levels, and SN ratio analysis was then applied for the parametric evaluations on the noise attenuation on specified frequencies in traffic noise (concentrated sound energy frequency range: 630–1000 Hz). Three optimal sets of design parameters on the interested frequencies, namely, 630 Hz, 800 Hz and 1000 Hz were obtained. ANOVA analysis was conducted to quantificationally identify the design parameters with statistical significance and remarkable contribution to the desired performance. Results indicate that the slit size has the most significant influence on the overall noise attenuation performance, followed by cavity width. An optimal set of design parameters to achieve the overall best noise reduction performance in the frequency range of 630–1000 Hz was finally determined by combining the SN ratio and ANOVA analysis. A prototype of the final optimized LRSCs window was then fabricated and tested in a semi-anechoic chamber. Good agreement was found between the experiment and numerical simulation. In comparison to the benchmark case, the final optimized design can achieve a further noise reduction by 2.84 dBA, 3.48 dBA and 5.56 dBA for the frequencies of 630 Hz, 800 Hz and 1000 Hz, respectively. The overall noise reduction for the interested frequency range can be promoted by 3.28 dBA. The results indicate that the proposed optimization methodology is practical and efficient in designing a high-performance LRSCs window or improving similar applications.
The First Data on Parasitic Arthropods of the European Bison in the Summer Season with a World Checklist
Authors: Izdebska Joanna N. , Rolbiecki Leszek , Bielecki Wojciech
Published: January 2022

The European bison (wisent) Bison bonasus is not only the largest terrestrial mammal in Europe, but also an example of the successful restitution and reintroduction of a species that became extinct in nature at the beginning of the 20th century. Even so, it remains a rare, protected species, and one susceptible to varied threats, including diseases. It has a specific parasitofauna, comprising only a few specific species retained with the last hosts in the restitution process, and some others acquired from other ungulates, or via environmental interactions. The current data on parasitic arthropods originated from the winter season, and less frequently, from autumn and early spring. The present study contains the first information on the occurrence of arthropods in the European bison (n = 6) in the summer season, which is the period of their increased activity. Data on the seasonal and populational dynamics of ectoparasites have been verified; specific parasite species (Demodex bisonianus, Bisonicola sedecimdecembrii, and recently described Demodex bialoviensis) and typical parasite species (Chorioptes bovis, Ixodes ricinus) have been recorded. The data refer to the information from other study periods, summarizing and valorizing data on parasitic arthropods in the form of a global checklist. These arthropods occur in European bison frequently (prevalence reaches up to 100%), but they are present in small numbers, and do not present a burden for the host; only sometimes, with high intensity of infection, they cause parasitoses (chorioptosis, mallohagosis). However, as an effect of regular activity, by penetrating the skin of the host, they create gateways for secondary infections, and hematophagous parasites (e.g., ticks) can be vectors of pathogens that can be hazardous, even at low infection levels. The identification of the parasitological threats faced by European bison is not only important from the standpoint of the study object, i.e., the Białowieża Forest European bison population, from which the existing bison population of Europe has been derived, but also the fact that their transfer to different parts of Europe can entail the additional transfer of their parasites.
Colloid Carcinoma of the Pancreas with a Series of Radiological and Pathological Studies for Diagnosis: A Case Report
Authors: Chen Chuan-Han , Yeh Hong-Zen , Li Hsin-Ni
Published: January 2022

Pancreatic colloid carcinoma is an uncommon and unique malignancy possessing a significantly more favorable prognosis than that of ordinary pancreatic ductal adenocarcinoma. Accurate diagnosis of this rare entity is thus important for leading the ensuing optimal treatment. Herein we report a case of colloid carcinoma of the pancreas with a series of imaging findings and pathologic assessments. Being familiar with these radio-pathological features makes early diagnosis possible prior to operation.
A 13-Gene DNA Methylation Analysis Using Oral Brushing Specimens as an Indicator of Oral Cancer Risk: A Descriptive Case Report
Authors: Rossi Roberto , Gissi Davide B. , Gabusi Andrea , Fabbri Viscardo Paolo , Balbi Tiziana , Tarsitano Achille , Morandi Luca
Published: January 2022

Analysis of genetic or epigenetic markers from saliva or brushing specimens has been proposed as a diagnostic aid to identify patients at risk of developing oral cancer. However, no reliable non-invasive molecular method for this purpose is commercially available. In the present report, we describe the potential application of a procedure based on a 13-gene DNA methylation analysis using oral brushing samples from a patient affected by oral leukoplakia who developed two metachronous oral carcinomas during the follow-up period. A positive or a negative score was calculated for each brushing sample based on a predefined cut-off value. In this patient, a positive score was detected in the oral leukoplakia diagnosed more than 2 years before the development of oral squamous cell carcinoma and subsequently in clinically healthy mucosa 8 months before the appearance of a secondary tumor. This suggests a potential role of our procedure as an indicator of oral cancer risk.
Potential Role of Exosomes in the Chemoresistance to Gemcitabine and Nab-Paclitaxel in Pancreatic Cancer
Authors: Comandatore Annalisa , Immordino Benoit , Balsano Rita , Capula Mjriam , Garajovà Ingrid , Ciccolini Joseph , Giovannetti Elisa , Morelli Luca
Published: January 2022

In recent years, a growing number of studies have evaluated the role of exosomes in pancreatic ductal adenocarcinoma cancer (PDAC) demonstrating their involvement in a multitude of pathways, including the induction of chemoresistance. The aim of this review is to present an overview of the current knowledge on the role of exosomes in the resistance to gemcitabine and nab-paclitaxel, which are two of the most commonly used drugs for the treatment of PDAC patients. Exosomes are vesicular cargos that transport multiple miRNAs, mRNAs and proteins from one cell to another cell and some of these factors can influence specific determinants of gemcitabine activity, such as the nucleoside transporter hENT1, or multidrug resistance proteins involved in the resistance to paclitaxel. Additional mechanisms underlying exosome-mediated resistance include the modulation of apoptotic pathways, cellular metabolism, or the modulation of oncogenic miRNA, such as miR-21 and miR-155. The current status of studies on circulating exosomal miRNA and their possible role as biomarkers are also discussed. Finally, we integrated the preclinical data with emerging clinical evidence, showing how the study of exosomes could help to predict the resistance of individual tumors, and guide the clinicians in the selection of innovative therapeutic strategies to overcome drug resistance.
Photovoltaic Thermal Collectors Integrated with Phase Change Materials: A Comprehensive Analysis
Authors: Awad Muthanna Mohammed , Ahmed Omer Khalil , Ali Obed Majeed , Alwan Naseer T. , Yaqoob Salam J. , Nayyar Anand , Abouhawwash Mohamed , Alrasheedi Adel Fahad
Published: January 2022

The target of the current study was to review and analyze the research activities of previous studies on cooling techniques for thermal photovoltaic (PV) systems using phase-change materials. These materials have the ability to absorb and release certain amounts of potential heat energy by changing their state from phase to phase (solid–liquid) within a small temperature range. These materials have been used to regulate and lower the temperature, increase the efficiency, and extend the life of solar cells. A host of improvements have been made to phase-changing materials through the combined utilization of phase-change materials and fins in addition to nanoscale fluids to enhance electrical efficiency. When using PCMs, the thermal, electrical, and overall efficiency improved by 26.87%, 17.33%, and 40.59%, respectively. The addition of nanomaterials increased phase-change materials’ specific heat capacity and thermal conductivity, thus reducing the plate temperature and increasing the electrical efficiency. It was found that using of nanoparticles together with a microcapsule had better performance in terms of energy efficiency. Studies indicated that variable phase materials were not used because of their high cost and lack of stable operational design. Therefore, the effect of phase-change materials on PV/thermal (PVT) system performance needs further investigation and study.
Polarization-Insensitive Unit Cells for a Cost-Effective Design of a 3-D-Printed Fresnel-Lens Antenna
Authors: Moreno-Rodríguez Salvador , Balmaseda-Márquez Miguel A. , Carmona-Murillo Javier , Palomares-Caballero Ángel
Published: January 2022

A 3-D printed Fresnel-lens antenna formed by dielectric unit cells insensitive to polarization is presented in this article. The proposed unit cell can be implemented in any azimuth orientation, simplifying the design and the implementation of the Fresnel subzones, which is an advantage over the previous 3-D-printed Fresnel-lens designs. The unit cell exhibits a T-shaped geometry capable of providing no change in relative permittivity under TE polarizations orthogonal to each other. The novel design of the unit cell also provides robustness under oblique incidence and frequency. These features allow the radial arrangement of the unit cells to configure the subzones of the Fresnel lens, ensuring the desired relative permittivity. Additionally, the geometry of the printed unit cells enables self-supported subzones with the minimum number of unit cells per subzone. A 3-D-printed prototype of the proposed Fresnel lens was manufactured by stereolithography (SLA). The measurement results showed a good agreement with the simulated ones. The measured gain was 26.5 ± 0.5 dBi from 55 GHz to 65 GHz with a mean antenna efficiency of 79%.
Machine Learning Approach for Maximizing Thermoelectric Properties of BiCuSeO and Discovering New Doping Element
Authors: Parse Nuttawat , Pongkitivanichkul Chakrit , Pinitsoontorn Supree
Published: January 2022

Machine learning (ML) has increasingly received interest as a new approach to accelerating development in materials science. It has been applied to thermoelectric materials research for discovering new materials and designing experiments. Generally, the amount of data in thermoelectric materials research, especially experimental data, is very small leading to an undesirable ML model. In this work, the ML model for predicting ZT of the doped BiCuSeO was implemented. The method to improve the model was presented step-by-step. This included normalizing the experimental ZT of the doped BiCuSeO with the pristine BiCuSeO, selecting data for the BiCuSeO doped at Bi-site only, and limiting important features for the model construction. The modified model showed significant improvement, with the R2 of 0.93, compared to the original model (R2 of 0.57). The model was validated and used to predict the ZT of the unknown doped BiCuSeO compounds. The predicted result was logically justified based on the thermoelectric principle. It means that the ML model can guide the experiments to improve the thermoelectric properties of BiCuSeO and can be extended to other materials.
Heat Loss Reduction Approach in Cavity Receiver Design Based on Performance Investigation of a Novel Positive Conical Scheme
Authors: Na Xinchen , Yao Yingxue , Zhao Chenyang , Du Jianjun
Published: January 2022

The cavity receiver’s thermal conversion performance is critical for parabolic dish and tower Concentrated Solar Power (CSP) systems. Distinct from precedent research aiming to increase the receiver’s absorption through cavity geometry optimization, the objective of this work was to investigate the thermal conversion performance of a novel, positive conical cavity receiver design, following the heat loss reduction approach with simplified pipe forming, to stress the effectiveness of this approach in cavity receiver design, and to provide data for future optimization of the proposed design. To accomplish these goals, the novel receiver and existing designs’ heat flux absorption and heat loss are compared numerically. The resulting conversion power is also experimentally validated. The concept is inspired by analysis of formulas, suggesting the novel design may realize a thermal conversion improvement of 8.6%, at 650 K, and increases with the rise in temperature. The comprehensive numerical investigation combines ray tracing of identical incoming radiation to investigate the receiver absorption and CFD methods to investigate the cavities’ heat loss at identical temperatures. The absorption acquired is unoptimized. The novel design can reduce the heat loss by as much as 91.8% when compared with a negative conical design at 650 K, resulting in a 12.3% improvement in conversion power. The experimental investigation measures the energy conversion to the working fluid in different cavities under identical incoming radiation. The novel receiver outperforms by over 5.6% in the setup. After correcting boundary conditions using experiment measurements, the experimental and numerical results are comparable. This research proves that the novel positive conical receiver has a better thermal conversion performance over 650 K; thus, the heat loss reduction approach is effective and feasible in receiver designs within this temperature range.
Experiments with Pyrotechnic Compositions Based on a Mathematical Model: Part II Pyrotechnic Compositions Producing an Acoustic Effect with Optimum Properties
Authors: Biegańska Jolanta , Barański Krzysztof
Published: January 2022

The article presents a method of evaluating pyrotechnic compositions producing an acoustic effect. The aforementioned pyrotechnic compositions are used in firecrackers to simulate a cannon shot. Optimum compositions that were selected (Part I Evaluation of the applicability of mathematical models in developing pyrotechnic compositions producing an acoustic effect) for testing were evaluated for their explosive and physicochemical properties. The following methods were used to evaluate suitability: measurement of the sound intensity level, the burning rate, and sensitivity to mechanical stimuli—friction and flash fire temperature. In addition, a test concerning the change in the properties of pyrotechnic compositions during storage was also performed.
The Impact of Green Finance on Urban Haze Pollution in China: A Technological Innovation Perspective
Authors: Zeng Yiting , Wang Feng , Wu Jun
Published: January 2022

Green finance integrates the concept of environmental governance into the financial industry, which is conducive to sustainable development. Applying the mediating effect model, this paper investigates the effect of green finance on urban haze pollution and explores the mediating role of technological innovation of enterprises between them. Based on a sample of 639 enterprises in China over 2016–2019, a significantly negative effect of green finance on urban haze pollution is found. An increase of one standard deviation in green finance decreases PM2.5 concentration by 8.8 μg/m3, ceteris paribus. Further, green finance may improve environmental quality by promoting technological innovation. Considering the heterogeneity of enterprise ownership, this mediating effect exists in non-state-owned enterprises, while it cannot be observed in state-owned enterprises. This study proposes a new solution for pollution: using green financial tools to promote environmentally friendly technological progress.
Providing Convenient Indoor Thermal Comfort in Real-Time Based on Energy-Efficiency IoT Network
Authors: Brik Bouziane , Esseghir Moez , Merghem-Boulahia Leila , Hentati Ahmed
Published: January 2022

Monitoring the thermal comfort of building occupants is crucial for ensuring sustainable and efficient energy consumption in residential buildings. It enables not only remote real-time detection of situations, but also a timely reaction to reduce the damage made by harmful situations in targeted buildings. In this paper, we first design a new Internet of Things (IoT) architecture in order to provide remote availability of both indoor and outdoor conditions, with respect to the limited energy of IoT devices. We then build a multi-output prediction model of indoor parameters using a random forest learning algorithm, and based on a longitudinal real dataset of one year. Our prediction model considers outdoor conditions to predict the indoor ones. Hence, it helps to detect discomfort situations in real-time when comparing predicted variables to real ones. Furthermore, when detecting an indoor thermal discomfort, we provide a new genetic-based algorithm to find the most suitable values of indoor parameters, enabling the improvement of the indoor occupants’ thermal comfort. Numerical results show the efficiency of our prediction scheme, reaching an accuracy of 96%, as well as our genetic-based scheme in optimizing the indoor thermal parameters by 85%.
Delay Causes and Types in Nigerian Power Construction Projects
Authors: Ismaila Usman , Jung Wooyong , Park Chan Young
Published: January 2022

Numerous events cause delays to entire power projects’ completion timelines, leading to loss. This paper focuses on revealing the critical delay causes in Nigerian power projects and identifying the delay types such as excusable delay, compensable delay, critical delay, and concurrent delay from the different perspectives of owner and contractor. Based on the survey of the 84 industrial experts in power distribution and transmission projects in Nigeria, this study provides the mean score and rank of 39 delay causes. Among the causes, 14 are identified as critical in the Nigerian power project. The top three critical delay causes are corruption and bureaucracy in government, inadequate fund/budget allocation, and price fluctuation/inflation. Owner and contractor do not show the difference in classifying the 14 delay causes into excusable and compensable delays. However, there was a difference between owner and contractor in categorizing whether a cause is critical or not and concurrent or not. A different viewpoint of types of delay cause can lead to a dispute which may lead to other delays and losses, and thus it is meaningful to understand the types of delay perceived from owner and contractor. Even though the delay cause and types are different depending on an individual project, the results of this study serve as a reference and would be beneficial for the practitioners in the construction industry to help identify the delay causes to manage them effectively.
The Effect of Stand Density, Biodiversity, and Spatial Structure on Stand Basal Area Increment in Natural Spruce-Fir-Broadleaf Mixed Forests
Authors: Liu Di , Zhou Chaofan , He Xiao , Zhang Xiaohong , Feng Linyan , Zhang Huiru
Published: January 2022

Forest trees exhibit a large variation in the basal area increment (BAI), and the variation is attributed to the stand density, biodiversity, and stand spatial structure. Studying and quantifying the effect of these above variables on tree growth is vital for future forest management. However, the stand spatial structure based on neighboring trees has rarely been considered, especially in the mixed forests. This study adopted the random-forest (RF) algorithm to model and interpret BAI based on stand density, biodiversity, and spatial structure. Fourteen independent variables, including two stand density predictors, four biodiversity predictors, and eight spatial structure predictors, were evaluated. The RF model was trained for the whole stand, three tree species groups (gap, neutral, and shade_tolerant), and two tree species (spruce and fir). A 10-fold blocked cross-validation was then used to optimize the hyper-parameters and evaluate the models. The squared correlation coefficients (R2) for the six groups were 0.233 for the whole stand, 0.575 for fir, 0.609 for shade_tolerant, 0.622 for neutral, 0.722 for gap, and 0.730 for spruce. The Stand Density Index (SDI) was the most-important predictor, suggesting that BAI is primarily restricted by competition. BAI and species biodiversity were positively correlated for the whole stand. The stands were expected to be randomly distributed based on the relationship between the uniform angle index (W) and growth. The relationship between dominance (U) and BAI indicated that small trees should be planted around the light-demanding tree species and vice versa. Of note, these findings emphasize the need to consider the three types of variables in mixed forests, especially the spatial structure factors. This study may help make significant advances in species composition, spatial arrangement, and the sustainable development of mixed forests.
The Renaissance of Wild Food Plants: Insights from Tuscany (Italy)
Authors: Baldi Ada , Bruschi Piero , Campeggi Stephanie , Egea Teresa , Rivera Diego , Obón Concepción , Lenzi Anna
Published: January 2022

This paper provides an overview of wild food plants traditionally used in the gastronomy of Tuscany, an Italian region with high biological diversity and whose cultural heritage is well known. Forty-nine bibliographic sources, including five unpublished studies, were reviewed. A list of species with ecological characteristics, plant parts used, use category (food, liquor, or seasoning), methods of preparation (raw or cooked), and recipes is presented. The use of 357 taxa (3711 use reports, URs), was recorded, belonging to 215 genera and 72 botanical families. Over the total taxa, 12 are new for Tuscany, 52 seem not to be present in other Italian regions, and 54 were not detected in the consulted European ethnobotanical literature. Of these taxa, 324 (3117 URs) were used as food, while 49 (178 URs) and 81 (416 URs) were used for liquor and seasoning, respectively. Of the 17 different food recipes, cooked vegetables constituted the largest group, followed by salads, omelets, snacks, and fillings. The chemical composition of the recorded food plants and the possible safety risks associated to their consumption, as well as their traditional medicinal use, are also shown. This review highlights the richness of ethnobotanical knowledge in Tuscany. Such biocultural heritage can be a “source of inspiration” for agriculture. As a reservoir of potential new crops, wild edible flora may contribute to the development of emerging horticultural sectors such as vertical farming and microgreens production. Moreover, the nutrient content and healthy properties of many wild food plants reported in this study has the ability to meet consumer demand for functional foods.
Seismic and Newtonian Noise in the GW Detectors
Authors: Trozzo Lucia , Badaracco Francesca
Published: January 2022

Gravitational wave detectors aim to measure relative length variations of the order of ΔL/L≃10−21, or less. Thus, any mechanism that is able to reproduce such a tiny variation can, in principle, threaten the sensitivity of these instruments, representing a source of noise. There are many examples of such noise, and seismic and Newtonian noise are among these and will be the subject of this review. Seismic noise is generated by the incessant ground vibration that characterizes Earth. Newtonian noise is instead produced by the tiny fluctuations of the Earth’s gravitational field. These fluctuations are generated by variations of air and soil density near the detector test masses. Soil density variations are produced by the same seismic waves comprising seismic noise. Thus, it makes sense to address these two sources of noise in the same review. An overview of seismic and Newtonian noise is presented, together with a review of the strategies adopted to mitigate them.
Quantitative Real-Time Analysis of Differentially Expressed Genes in Peripheral Blood Samples of Hypertension Patients
Authors: Ali Fawad , Khan Arifullah , Muhammad Syed Aun , Hassan Syed Shams ul
Published: January 2022

Hypertension (HTN) is considered one of the most important and well-established reasons for cardiovascular abnormalities, strokes, and premature mortality globally. This study was designed to explore possible differentially expressed genes (DEGs) that contribute to the pathophysiology of hypertension. To identify the DEGs of HTN, we investigated 22 publicly available cDNA Affymetrix datasets using an integrated system-level framework. Gene Ontology (GO), pathway enrichment, and transcriptional factors were analyzed to reveal biological information. From 50 DEGs, we ranked 7 hypertension-related genes (p-value < 0.05): ADM, ANGPTL4, USP8, EDN, NFIL3, MSR1, and CEBPD. The enriched terms revealed significant functional roles of HIF-1-α transcription; endothelin; GPCR-binding ligand; and signaling pathways of EGF, PIk3, and ARF6. SP1 (66.7%), KLF7 (33.3%), and STAT1 (16.7%) are transcriptional factors associated with the regulatory mechanism. The expression profiles of these DEGs as verified by qPCR showed 3-times higher fold changes (2−ΔΔCt) in ADM, ANGPTL4, USP8, and EDN1 genes compared to control, while CEBPD, MSR1 and NFIL3 were downregulated. The aberrant expression of these genes is associated with the pathophysiological development and cardiovascular abnormalities. This study will help to modulate the therapeutic strategies of hypertension.
An Assessment of GPX1 (rs1050450), DIO2 (rs225014) and SEPP1 (rs7579) Gene Polymorphisms in Women with Endometrial Cancer
Authors: Janowska Magdalena , Potocka Natalia , Paszek Sylwia , Skrzypa Marzena , Żulewicz Kamila , Kluz Marta , Januszek Sławomir , Baszuk Piotr , Gronwald Jacek , Lubiński Jan , Zawlik Izabela , Kluz Tomasz
Published: January 2022

Background: Numerous studies indicate a relationship between the presence of GPX1 (rs1050450), DIO2 (rs225014) and SEPP1 (rs7579) gene polymorphisms and the development of chronic or neoplastic diseases. However, there are no reports on the influence of these polymorphisms on the development of endometrial cancer. Methods: 543 women participated in the study. The study group consisted of 269 patients with diagnosed endometrial cancer. The control group consisted of 274 healthy women. Blood samples were drawn from all the participants. The PCR-RFLP method was used to determine polymorphisms in the DIO2 (rs225014) and GPX1 (rs1050450) genes. The analysis of polymorphisms in the SEPP1 (rs7579) gene was performed by means of TaqMan probes. Results: There was a 1.99-fold higher risk of developing endometrial cancer in CC homozygotes, DIO2 (rs225014) polymorphism (95% Cl 1.14–3.53, p = 0.017), compared to TT homozygotes. There was no correlation between the occurrence of GPX1 (rs1050450) and SEPP1 (rs7579) polymorphisms and endometrial cancer. Conclusion: Carriers of the DIO2 (rs225014) polymorphism may be predisposed to the development of endometrial cancer. Further research confirming this relationship is recommended.
Functional Innovation through Gene Duplication Followed by Frameshift Mutation
Authors: Guo Baocheng , Zou Ming , Sakamoto Takahiro , Innan Hideki
Published: January 2022

In his influential book “Evolution by Gene Duplication”, Ohno postulated that frameshift mutation could lead to a new function after duplication, but frameshift mutation is generally thought to be deleterious, and thus drew little attention in functional innovation in duplicate evolution. To this end, we here report an exhaustive survey of the genomes of human, mouse, zebrafish, and fruit fly. We identified 80 duplicate genes that involved frameshift mutations after duplication. The frameshift mutation preferentially located close to the C-terminus in most cases (55/88), which indicated that a frameshift mutation that changed the reading frame in a small part at the end of a duplicate may likely have contributed to adaptive evolution (e.g., human genes NOTCH2NL and ARHGAP11B) otherwise too deleterious to survive. A few cases (11/80) involved multiple frameshift mutations, exhibiting various patterns of modifications of the reading frame. Functionality of duplicate genes involving frameshift mutations was confirmed by sequence characteristics and expression profile, suggesting a potential role of frameshift mutation in creating functional novelty. We thus showed that genomes have non-negligible numbers of genes that have experienced frameshift mutations following gene duplication. Our results demonstrated the potential importance of frameshift mutations in molecular evolution, as Ohno verbally argued 50 years ago.
The Difference in the Proportions of Deleterious Variations within and between Populations Influences the Estimation of FST
Authors: Subramanian Sankar
Published: January 2022

Estimating the extent of genetic differentiation between populations is an important measure in population genetics, ecology and evolutionary biology. The fixation index, or FST, is an important measure, which is routinely used to quantify this. Previous studies have shown that the FST estimated for selectively constrained regions was significantly lower than that estimated for neutral regions. By deriving the theoretical relationship between FST at neutral and constrained sites, we show that excess in the fraction of deleterious variations segregating within populations compared to those segregating between populations is the cause for the reduction in FST estimated at constrained sites. Using whole-genome data, our results revealed that the magnitude of reduction in FST estimates obtained for selectively constrained regions was much higher for distantly related populations compared to those estimated for closely related pairs. For example, the reduction was 47% for comparison between Europeans and Africans, 30% for the European and Asian comparison, 16% for the Northern and Southern European pair, and only 4% for the comparison involving two Southern European (Italian and Spanish) populations. Since deleterious variants are purged over time due to purifying selection, their contribution to the among-population diversity at constrained sites decreases with the increase in the divergence between populations. However, within-population diversities remain the same for all pairs compared; therefore, the FST estimated at constrained sites for distantly related populations are much smaller than those estimated for closely related populations. We obtained similar results when only the SNPs with similar allele frequencies at neutral and constrained sites were used. Our results suggest that the level of population divergence should be considered when comparing constrained site FST estimates from different pairs of populations.
Comparative Analysis of Mitochondrial Genomes in Two Subspecies of the Sunwatcher Toad-Headed Agama (Phrynocephalus helioscopus): Prevalent Intraspecific Gene Rearrangements in Phrynocephalus
Authors: Wu Na , Liu Jinlong , Wang Song , Guo Xianguang
Published: January 2022

Intraspecific rearrangements of mitochondrial genomes are rarely reported in reptiles, even in vertebrates. The sunwatcher toad-headed agama, Phryncoephalus helioscopus, can serve as an excellent model for investigating the dynamic mitogenome structure at intraspecific level. To date, seven subspecies of P. helioscopus are well recognized, but little is known about the mitogenomic evolution among different subspecies. In this study, complete mitogenomes of subspecies P. helioscopus varius II and P. helioscopus cameranoi were determined by next-generation sequencing, and another P. helioscopus varius I retrieved from GenBank was compiled for comparative analysis. The nucleotide composition and the codon usage are similar to those previously published from toad-headed agamas. P. helioscopus varius II and P. helioscopus cameranoi have 23 tRNA genes, including standard 22 tRNA genes and one extra tRNA-Phe (tRNA-Phe duplication). Gene order and phylogenetic analyses in the genus Phrynocephalus support prevalent intraspecific gene rearrangement in P. helioscopus and other congener species including P. erythrurus, P. vlangalii, and P. forsythii. Six different mitochondrial gene arrangements are observed in Phrynocephalus. Overall, the occurrence of rearrangements may result from multiple independent structural dynamic events. The split of the two subspecies in P. helioscopus was dated at approximately 2.34 million years ago (Ma). Two types of gene rearrangements are found in the three mitogenomes of P. helioscopus, and this intraspecific rearrangement phenomenon can be explained by the tandem duplication/random loss (TDRL) model. Post duplication, the alternative loss types can occur in 0.23–0.72 Ma, suggesting that the duplication and fixation of these rearrangements can occur quite quickly. These findings highlight the need for more mitogenomes at the population level in order to better understand the potentially rampant intraspecific mitogenomic reorganization in Phrynocephalus.
Global Precedent-Based Extrapolation Estimate of the M8+ Earthquake Hazard (According to USGS Data as of 1 June 2021)
Authors: Malyshev Aleksandr , Malysheva Lidiia
Published: January 2022

The paper describes the algorithm and the results of the seismic hazard estimate based on the data of the seismological catalog of the US Geological Survey (USGS). The prediction algorithm is based on the search for clusters of seismic activity in which current activity trends correspond to foreshock sequences recorded before strong earthquakes (precedents) that have already occurred. The time of potential hazard of a similar earthquake is calculated by extrapolating the detected trends to the level of activity that took place at the time of the precedent earthquake. It is shown that the lead time of such a forecast reaches 10–15 years, and its implementation is due to the preservation and stability of the identified trends. The adjustment of the hazard assessment algorithm was carried out in retrospect for seven earthquakes (M8+) that had predictability in foreshock preparation. The evolution of the potential seismic hazard from 1 January 2020 to 1 June 2021 has been traced. It is concluded that precedent-based extrapolation assessments have prospects as a tool designed for the early detection and monitoring of potentially hazardous seismic activity.
Watermelon Rind and Flesh Volatile Profiles and Cultivar Difference
Authors: Du Xiaofen , Ramirez Jessica
Published: January 2022

Watermelon rind is treated as agricultural waste and commonly discarded, causing environmental issues and biomass loss. This study aimed to identify volatile profiles of watermelon rind and flesh and their cultivar difference. Volatiles were analyzed using solid-phase microextraction–gas chromatography–mass spectrometry (SPME-GC-MS). A total of 132 volatiles were identified, including aldehydes, alcohols, ketones, terpenes/terpenoids, esters, lactones, acids, and sulfides. In both rind and flesh, the most dominant compounds in numbers and abundance (peak area) were aldehydes and alcohols, which accounted 94–96% of the total volatile abundance in the rind and 85–87% in the flesh. Total volatile in watermelon rind was only 28–58% of the corresponding flesh samples. Both rind and flesh shared nine-carbon aldehydes and alcohols, though the rind lacked additional diversity. Volatile difference between rind and flesh was greater than the difference among cultivars, although volatiles in the rind could be two times difference between Fascination and other three watermelons (Captivation, Exclamation, and Excursion). This study provides the first-hand knowledge regarding watermelon rind volatile profiles and cultivar difference and shows the potential use of rind in food or beverages due to its naturally contained nine-carbon compounds.
Root-Knot Nematode Species Associated with Horticultural Crops in the Island of Azores, Portugal
Authors: Rusinque Leidy , Nóbrega Filomena , Cordeiro Laura , Lima Arlindo , Andrade Samuel , Inácio Maria Lurdes
Published: January 2022

Plant-parasitic nematodes (PPN) are an economically important group of plant pests present throughout the world. In particular, root-knot nematodes (RKN), sedentary endoparasites of a wide variety of economically important hosts with the ability to survive in temperate regions. During 2020–2021 an extensive survey of Meloidogyne spp. was undertaken on the island of São Miguel Azores, Portugal. A total of 80 samples comprising 23 species of plants were collected from 13 localities in 4 districts of the island. Samples included field and greenhouse vegetable crops. Bioassays were carried out to obtain females and juveniles for morphological and biochemical characterization of the isolates. The observed morphological features showed high similarity and consistency with previous descriptions of the genus. Concerning the biochemical characterization, the esterase (EST) phenotype displayed the patterns described for M. incognita, M. arenaria, and M. javanica. Meloidogyne incognita was found to be the most prevalent species with respect to both natural host range and geographical distribution, followed by M. arenaria and M. javanica. This is the first field survey that assesses the species of root-knot nematodes associated with horticultural crops in the Azores Island, contributing additional information on the distribution of this genus.
Changes in Physical Activity and the Occurrence of Specific Symptoms of “Long-COVID Syndrome” in Men Aged 18–25
Authors: Sojka Anna , Machniak Mariusz , Andrzejewski Waldemar , Kosendiak Aureliusz , Chwałczyńska Agnieszka
Published: January 2022

The aim of this study was to assess the occurrence of non-specific symptoms of “long-COVID syndrome” depending on the physical activity undertaken resulting from the imposed forms of study (distance learning–contact learning); 136 men aged 21.5 ± 1.58 from universities educating students of medical faculties were examined. The difference between the universities was mainly due to the nature of the classes undertaken (classes remotely-hybrid form) in the period from March 2020 to February 2021. Among the respondents, 17% in Group I and 16% in Group II were infected with the SARS-CoV-2 virus, including 50% in Group I with moderate symptoms, and in Group II—most people 45% with mild symptoms. The conducted research clearly shows the impact of the COVID-19 pandemic on students. They show a number of important problems, such as reduced physical activity, as well as increased body weight and time spent in front of the monitor. They also make it clear that the health consequences of the pandemic affect both people who were infected with the SARS-CoV-2 virus and those who did not suffer from this infection.