bluuebunny/crossref_metadata_2025 huggingface dataset:
HLA-G 14 bp Ins/Del (rs66554220) Variant Is Not Associated with Breast Cancer in Women from Western Mexico
Authors: Becerra-Loaiza Denisse Stephania , Roldan Flores Luisa Fernanda , Ochoa-Ramírez Luis Antonio , Gutiérrez-Zepeda Bricia M. , Del Toro-Arreola Alicia , Franco-Topete Ramón Antonio , Morán-Mendoza Andrés , Oceguera-Villanueva Antonio , Topete Antonio , Javalera David , Quintero-Ramos Antonio , Daneri-Navarro Adrián
Published: August 2023

HLA-G is a physiology and pathologic immunomodulator detrimentally related to cancer. Its gene is heavily transcriptionally and post-transcriptionally regulated by variants located in regulator regions like 3′UTR, being the most studied Ins/Del of 14-bp (rs66554220), which is known to influence the effects of endogen cell factors; nevertheless, the reports are discrepant and controversial. Herein, the relationship of the 14-bp Ins/Del variant (rs66554220) with breast cancer (BC) and its clinical characteristics were analyzed in 182 women with non-familial BC and 221 disease-free women as a reference group. Both groups from western Mexico and sex–age-matched (sm-RG). The rs66554220 variant was amplified by SSP-PCR and the fragments were visualized in polyacrylamide gel electrophoresis. The variant rs66554220 was not associated with BC in our population. However, we suggest the Ins allele as a possible risk factor for developing BC at clinical stage IV (OR = 3.05, 95% CI = 1.16–7.96, p = 0.01); nevertheless, given the small stratified sample size (n = 11, statistical power = 41%), this is inconclusive. In conclusion, the 14-bp Ins/Del (rs66554220) variant of HLA-G is not associated with BC in the Mexican population, but might be related to advanced breast tumors. Further studies are required.
Green Synthesis and Antibacterial Activity of Silver Nanoparticles Obtained from Moringa oleifera Seed Cake
Authors: Coelho Nuno , Jacinto João P. , Silva Rodrigo , Soares Jéssica C. , Pereira Alice S. , Tavares Pedro
Published: August 2023

In the present work, we report a simple, cost-efficient, and eco-friendly green method to synthesize silver nanoparticles with antimicrobial activity. An ethanolic extract from Moringa oleifera seed residue was used as a reducing and stabilizing agent in an aqueous solution of silver nitrate. The synthesized silver nanoparticles’ hydrodynamic radius, polydispersity index, and zeta-potential were evaluated by Dynamic Light Scattering. Scanning Electron Microscopy was employed to confirm the size and morphology of the nanoparticles. Synthesis of spherical particles with 127 ± 24 nm was confirmed. After sintering, the product of the synthesis was analyzed by X-ray diffraction. The X-ray diffraction pattern attributed to reflections of the (111), (200), (220), and (311) planes, which are characteristic of silver nanoparticles, confirms the successful synthesis of crystalline face-centered cubic nanoparticles. The antimicrobial activity of the bionanoparticles was tested against Escherichia coli BL21(DE3) cells and compared with the effect of a Moringa oleifera seed cake extract. Herein, we show that the growth of Escherichia coli is significantly affected by the addition of the synthesized bionanoparticles. Addition of the bionanoparticles inhibited the growth and lengthened the lag phase of the bacterial culture.
Corrosion Hazards in Urban Infrastructure Structures Using the Example of the Al Bayt Stadium in Katar
Authors: Krolikowska Agnieszka , Bonora Pier Luigi
Published: August 2023

A significant aspect of corrosion failures and catastrophes originate from trivial mistakes in either the choice or connection of different materials, as well as from inaccurate evaluation of the compatibility between materials and the environment. The example shown in the present paper summarizes several wrong solutions due to a lack of knowledge of the basic rules of corrosion control. By chance, the consequences of these errors already appeared during construction; therefore, they were not able to cause damage during operation. This paper is the third in a series devoted to enhancing the need for professional corrosion control design for infrastructures.
Pm2.5 Time Series Imputation with Deep Learning and Interpolation
Authors: Flores Anibal , Tito-Chura Hugo , Centty-Villafuerte Deymor , Ecos-Espino Alejandro
Published: August 2023

Commonly, regression for time series imputation has been implemented directly through regression models, statistical, machine learning, and deep learning techniques. In this work, a novel approach is proposed based on a classification model that determines the NA value class, and from this, two types of interpolations are implemented: polynomial or flipped polynomial. An hourly pm2.5 time series from Ilo City in southern Peru was chosen as a study case. The results obtained show that for gaps of one NA value, the proposal in most cases presents superior results to techniques such as ARIMA, LSTM, BiLSTM, GRU, and BiGRU; thus, on average, in terms of R2, the proposal exceeds implemented benchmark models by between 2.4341% and 19.96%. Finally, supported by the results, it can be stated that the proposal constitutes a good alternative for short-gaps imputation in pm2.5 time series.
Preparation and Properties of MgO-Al2O3-SiO2 Glass–Ceramics with Controllable Crystalline Phases
Authors: Pei Zhen , Huang Haixiao , Guo Xingzhong , Zhang Jinming , Chen Mingxing
Published: August 2023

MgO–Al2O3–SiO2 (MAS) glass–ceramics with controllable crystalline phases were successfully prepared using the melting method followed by heat treatment. The effects of the main components of glass on the crystallisation kinetics, nucleation, crystallisation and properties of glass–ceramics were investigated in detail. As the Al2O3 and MgO contents increase and SiO2 content decreases, the crystallisation peak temperature and activation energy of MAS glass decrease, while the crystal growth tends to follow a homogeneous nucleation crystallisation. The MAS glass nucleation temperature and time increase with higher concentrations of Al2O3 and MgO and with a lower SiO2 concentration. Mg2(Al4Si5O18) indialite and MgAl2O4 spinel precipitate simultaneously in the MAS glass after crystallisation; the relative proportion of crystalline phases is related to the composition and crystallisation temperature. A higher SiO2 content allows the formation of a dominant indialite phase, while higher MgO and Al2O3 contents promote the formation of a dominant spinel phase. The MAS glass ceramic with a greater indialite phase has good dielectric properties with a dielectric constant of 6.499 and dielectric loss of 0.0064, while that of a higher spinel phase possesses improved mechanical properties, with a Vickers hardness of 715 Hv and a bending strength of 244.9 MPa.
Eccrine Poroma: Pathogenesis, New Diagnostic Tools and Association with Porocarcinoma—A Review
Authors: Kyrmanidou Eirini , Fotiadou Christina , Kemanetzi Christina , Trakatelli Myrto-Georgia , Trigoni Anastasia , Patsatsi Aikaterini , Apalla Zoe , Lazaridou Elizabeth
Published: August 2023

Eccrine poroma (EP) is a relatively rare benign adnexal neoplasm that usually affects elderly patients. Its pathogenesis is still under investigation, but recent gene studies have revealed gene fusions as key incidences resulting in oncogenetic pathways. It often presents as a solitary, firm papule, mostly asymptomatic, located on the soles or palms. Due to its clinical and dermoscopic variability, it is characterized as the great imitator. We performed a literature review, aiming to summarize current data on the pathogenetic mechanisms, new dermoscopic features, and novel diagnostic tools that may aid in early diagnosis and proper management of this rare adnexal tumor. Furthermore, we reviewed the possible pathogenetic associations between EP and its malignant counterpart, namely eccrine porocarcinoma. This systematic approach may aid in understanding the pathogenetic mechanisms and how to use novel histopathologic markers and imaging methods to overcome the diagnostic dilemma of this rare tumor.
A Rare Case of Post-Traumatic Cervical Ligamentous Tear Complicated by Vertebral Arteriovenous Fistula (vAVF), with Successful Endovascular Treatment
Authors: Fazdlin Abdul Rahim Nur , Rizuana Iqbal Hussain , Ch’ng Li Shyan
Published: August 2023

Post-traumatic vertebral arteriovenous fistula (vAVF) caused by motor vehicle accidents (MVA) is a rare condition in which there is abnormal communication between the vertebral artery and its adjacent veins. In a post-MVA setting, it is commonly associated with vertebral body fracture. In this paper, we report a case of a 19-year-old girl with a complete C2/C3 anterior and posterior ligament tear post MVA without any cervical bony injury. Initial plain computed tomography (CT) cervical scan showed a prevertebral hematoma. A CT angiogram (CTA) raised the suspicion of a pseudo-aneurysm at the right posterior C3 vertebral body. Further imaging with magnetic resonance imaging (MRI) demonstrated traumatic AVF at the C2/C3 level involving the V2/V3 right vertebral artery to the vertebral venous plexus. Digital Subtraction Angiography (DSA) further revealed a transected right vertebral artery at the C2/C3 level with an arteriovenous fistula and an enlarged vertebral venous plexus. The fistulous communication was successfully occluded with coils from a cranial and caudal approach to the transected segment right vertebral artery, with a total of eight coils. Post-MVA vertebral arteriovenous fistula (vAVF) is a rare sequela of vertebral bony injury at the cervical region, and is an even rarer association with an isolated ligamentous injury, whereby endovascular treatment with ipsilateral vertebral artery closure is a feasible treatment of vAVF.
Hybrid DAER Based Cross-Modal Retrieval Exploiting Deep Representation Learning
Authors: Huang Zhao , Hu Haowu , Su Miao
Published: August 2023

Information retrieval across multiple modes has attracted much attention from academics and practitioners. One key challenge of cross-modal retrieval is to eliminate the heterogeneous gap between different patterns. Most of the existing methods tend to jointly construct a common subspace. However, very little attention has been given to the study of the importance of different fine-grained regions of various modalities. This lack of consideration significantly influences the utilization of the extracted information of multiple modalities. Therefore, this study proposes a novel text-image cross-modal retrieval approach that constructs a dual attention network and an enhanced relation network (DAER). More specifically, the dual attention network tends to precisely extract fine-grained weight information from text and images, while the enhanced relation network is used to expand the differences between different categories of data in order to improve the computational accuracy of similarity. The comprehensive experimental results on three widely-used major datasets (i.e., Wikipedia, Pascal Sentence, and XMediaNet) show that our proposed approach is effective and superior to existing cross-modal retrieval methods.
The Role of Soils in Sustainability, Climate Change, and Ecosystem Services: Challenges and Opportunities
Authors: Telo da Gama José
Published: August 2023

Soils are crucial life supporters and nutrient providers, their functionality impacted by their nutritional balance, pH, and organic matter content. These substrates help regulate water, support diverse organisms, and play a critical role in carbon sequestration, especially in the context of climate change. However, soils are under threat from anthropogenic and climatic pressures, warranting active resource management strategies. The European Union (EU) has acknowledged soil sustainability’s importance, encouraging eco-friendly agricultural practices and enhanced soil carbon storage. However, the criticality of soils is often overlooked when establishing global sustainable development goals. Counteracting soil degradation is key in battling desertification and influenced by factors like unsustainable agriculture, deforestation, and poor irrigation. Innovative solutions like circular economy approaches and sustainable biomass utilization are necessary to reduce greenhouse gas emissions. Also, improving dietary habits and reducing food waste can help mitigate the environmental impact of food consumption, with a shift towards plant-based proteins being more sustainable. Addressing these challenges will contribute to a more sustainable and resilient future.
A Small Country with Big Ambitions: Does This Include the Gifted?
Authors: Sutherland Margaret , Reid Catherine
Published: August 2023

Scotland is a small country with an education system whose roots lie within an inclusive and egalitarian approach to the education of young people. Subsequent legislation, policies, and curriculum frameworks have been influenced by this, and also by the international move toward equitable, inclusive, and quality lifelong learning for all. Supporting those who are highly able/gifted and talented against such a backdrop offers both opportunities and challenges. In this qualitative study, the Global Principles for Professional Learning in Gifted Education are used to interrogate recent key legislation; the current curriculum framework, Curriculum for Excellence, and the National Framework for Inclusion; to ascertain the extent to which this inclusive approach, on paper, affords in-class and school-based support for gifted and talented/highly able learners. The results indicate that the legislative and policy frameworks coalesce with the Global Principles. While legislation does not change practice, it does influence and shape practice, and so can be used as a springboard for developing dynamic, culturally appropriate opportunities for Scotland’s gifted young people.
HEBCS: A High-Efficiency Binary Code Search Method
Authors: Sun Xiangjie , Wei Qiang , Du Jiang , Wang Yisen
Published: August 2023

Binary code search is a technique that involves finding code with similarity to a given code within a code database. It finds extensive application in scenarios such as vulnerability queries and code defect analysis. While many existing methods employ advanced machine learning models for similarity analysis, their lack of interpretability and low efficiency in dealing with large-scale functions still remain challenges. To address these issues, we propose a high-efficiency binary code search method called HEBCS. It employs an interpretable approach to extract function-level features and transforms each feature into a locality-sensitive hash representation. Then, the hashes of these features are combined to form the hash of the function. By leveraging the pigeonhole principle, HEBCS enables efficient storage and retrieval of functions, ensuring high execution efficiency even in the presence of large-scale data. Furthermore, we compare HEBCS with a classic method and a state-of-the-art method, demonstrating that HEBCS achieves significantly higher search efficiency while maintaining a comparable accuracy, recall and F1-score. In real-world vulnerability query applications, HEBCS demonstrated promising results. Its effectiveness in large-scale binary function searches suggests significant potential for practical applications.
A Critical Review of Techniques for the Experimental Extraction of the Thermal Resistance of Bipolar Transistors from DC Measurements—Part I: Thermometer-Based Approaches
Authors: d’Alessandro Vincenzo , Catalano Antonio Pio , Scognamillo Ciro , Müller Markus , Schröter Michael , Zampardi Peter J. , Codecasa Lorenzo
Published: August 2023

This paper presents a critical and detailed overview of experimental techniques for the extraction of the thermal resistance of bipolar transistors from simple DC current/voltage measurements. More specifically, this study focuses on techniques based on a thermometer, i.e., the relation between the base-emitter voltage and the junction temperature. The theory behind the techniques is described with a unified and comprehensible nomenclature. Advantages, underlying approximations, and limitations of the methods are illustrated. The accuracy is assessed by emulating the DC measurements with PSPICE electrothermal simulations of a transistor model, applying the techniques to the simulated currents/voltages, and comparing the extracted thermal resistance data with the values obtained from the target formulation embedded in the transistor model. An InGaP/GaAs HBT and an Si/SiGe HBT for high-frequency applications are considered as case-studies.
Structural Performance-Based Design Optimisation of a Secondary Mirror for a Concentrated Solar Power (CSP) Plant
Authors: Pinello Lucio , Fossati Massimo , Giglio Marco , Cadini Francesco , Bevilacqua Carla , Cilento Mario , Bassetti Fulvio , Magaldi Raffaello
Published: August 2023

Concentrated Solar Power (CSP) plants use mirrors to reflect and concentrate sunlight onto a receiver, to heat a fluid and store thermal energy, at high temperature and energy density, to produce dispatchable heat and/or electricity. The secondary mirror is a critical component in the optical system of certain Solar Power Tower plants (SPT), as it redirects the concentrated sunlight from the primary mirror onto the receiver, which can be arranged at ground level. In this study, we propose a design optimisation for the secondary mirror of a CSP plant. The design optimisation method consists of two steps. The first step involves the use of the finite element simulation software Abaqus 2022 to analyse the structural performance of the secondary mirror under thermal loads and wind. The second step consists of the use of simulation results to identify the combination of design parameters and best performances, with respect to both design constraints and structural safety. This is carried out by developing an algorithm that selects those configurations which satisfy the constraints by using safety coefficients. The proposed optimisation method is applied to the design of a potential configuration of a secondary mirror for the beam-down of the CSP Magaldi STEM® technology, although the methodology can be extended to other components of CSP plants, such as primary mirrors and receivers, to further enhance the structural performance of these systems.
Analysis of Discharge Failure Mechanism of IGBT Power Modules
Authors: Xu Lu , Li Kaixuan , Yang Ziyue , Jiang Xinyu
Published: August 2023

IGBT power modules are usually used as circuit-breaking components in power systems, and are widely used in solid-state DC circuit breakers, hybrid DC circuit breakers, all-electric aircraft, high-speed railways, new energy vehicles, and power transmission systems. In these systems, IGBT power modules are usually faced with extremely harsh working conditions and there is a failure risk. Insulation degradation should be a cause for concern as a potential path of power module failure. In this paper, the discharge phenomena of the IGBT power module were observed based on Intensified Charge Coupled Devices (ICCD), and the triple junctions composed of copper–ceramic–silicone gel inside IGBT were found as the discharge points. Furthermore, the directed bonded copper (DBC) ceramic filled with silicone gel was used as a test sample to study the discharge failure process, including the partial discharge (PD), surface charges, and electric trees. The mechanism of discharge failure is discussed and analyzed. The insulation degradation process is accompanied by phenomena such as severe partial discharge and rapid electric tree growth. This research provides support for the analysis idea and guidance of the research method for the cause of power module failure.
Study on the Effect of Exhaust Gas Recirculation Coupled Variable Geometry Turbocharger and Fuel Quantity Control on Transient Performance of Turbocharged Diesel Engine
Authors: Gu Wenyu , Su Wanhua
Published: August 2023

With increasingly stringent emissions regulations, there are growing demands for the transient performance of diesel engines. This study conducted a transient bench test on a two-stage turbocharged heavy-duty diesel engine to optimize its performance during a load increase (20% to 100% in 1 s) at a constant speed (1200 RPM) transient process. The results showed that the transient control scheme using the low-pressure EGR system resulted in a 42.1% reduction in the peak value of soot emission, a 24.8% decrease in the peak value of NOx emission, a 9.14% decrease in ISFC and a 30.6% increase in maximum IMEP achieved in 1 s, compared to the steady-state optimization control scheme without EGR. Transient control scheme using the high-pressure EGR system resulted in a 24.4% reduction in the peak value of soot emission, a 31.8% reduction in the peak value of NOx emission, a 9.52% reduction in ISFC, and a 31.7% increase in maximum IMEP achieved in 1 s. The comparison of high and low-pressure EGR systems revealed that the low-pressure EGR system produced lower compromising emissions, while alterations in control parameters for the diesel engine with a high-pressure EGR system had a more significant impact on the transient process performance.
Comparison of Parameter Estimation Methods Based on Two Additive Biomass Models with Small Samples
Authors: Xiong Nina , Qiao Yue , Ren Huiru , Zhang Li , Chen Rihui , Wang Jia
Published: August 2023

Accurately estimating tree biomass is crucial for monitoring and managing forest resources, and understanding regional climate change and material cycles. The additive model system has proven reliable for biomass estimation in Chinese forestry since it considers the inherent correlation among variables based on allometric equations. However, due to the increasing difficulty of obtaining a substantial amount of sample data, estimating parameters for the additive model equations becomes a formidable challenge when working with limited sample sizes. This study primarily focuses on analyzing these parameters using data extracted from a smaller sample. Here, we established two additive biomass model systems using the independent diameter and the combined variable that comprises diameter and tree height. The logarithmic Nonlinear Seemingly Uncorrelated (logarithmic NSUR) method and the Generalized Method of Moments (GMM) method were applied to estimate the parameters of these models. By comparing four distinct approaches, the following key results were obtained: (1) Both the GMM and logarithmic NSUR methods can yield satisfactory goodness of fit and estimation precision for the additive biomass equations, with the root mean square error (RMSE) were significantly low, and coefficients of determination (R2) were mostly higher than 0.9. (2) Comparatively, examining the fitted curves of predicted values, the GMM method provided better fitting than the NSUR method. The GMM method with the combined variable is the most suggested approach for the calculation and research of single-tree biomass models with a small sample size.
Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces
Authors: Minor Martha , Sabillón Luis
Published: August 2023

The use of blue light-emitting diodes (LEDs) is emerging as a promising dry decontamination method. In the present study, LEDs emitting ultra-high irradiance (UHI) density at 405 nm (842 mW/cm2) and 460 nm (615 mW/cm2) were used to deliver high-intensity photoinactivation treatments ranging from 221 to 1107 J/cm2. The efficacy of these treatments to inactivate E. coli O157:H7 dry cells was evaluated on clean and soiled stainless steel and cast-iron surfaces. On clean metal surfaces, the 405 and 460 nm LED treatment with a 221 J/cm2 dose resulted in E. coli reductions ranging from 2.0 to 4.1 log CFU/cm2. Increasing the treatment energy dose to 665 J/cm2 caused further significant reductions (>8 log CFU/cm2) in the E. coli population. LED treatments triggered a significant production of intracellular reactive oxygen species (ROS) in E. coli cells, as well as a significant temperature increase on metal surfaces. In the presence of organic matter, intracellular ROS generation in E. coli cells dropped significantly, and treatments with higher energy doses (>700 J/cm2) were required to uphold antimicrobial effectiveness. The mechanism of the bactericidal effect of UHI blue LED treatments is likely to be a combination of photothermal and photochemical effects. This study showed that LEDs emitting monochromatic blue light at UHI levels may serve as a viable and time-effective method for surface decontamination in dry food processing environments.
Two-Dimensional Fractional Order Iterative Learning Control for Repetitive Processes
Authors: Zhang Bitao , Luo Haobo
Published: August 2023

The convergence and robustness rejecting parameters variations and external disturbance of the system are crucial for repetitive processes. In this paper, a two-dimensional robust fractional-order iterative learning control (FOILC) is proposed for the repetitive motion process to enhance the convergence and robustness. A fractional-order proportional derivative function (FOPDF) is designed as the control variable to replace the tracking error of the integer-order iterative learning control (IOILC) algorithm. The required dynamic output fractional-order iterative learning controller is constructed by solving a set of linear matrix inequalities (LMI), and the control parameters are adjusted according to the given specifications. Simulation and experimental results in robot torque control are given to prove the effectiveness and feasibility of the proposed design method.
Phytophthora Root Rot in Rangpur Lime Cultivated in Soil Managed with Ecological and Conventional Mower
Authors: Prates Caio Barbosa , Feichtenberger Eduardo , Martinelli Rodrigo , Gadanhoto Biana Pelissari , Devite Fernando Trevizan , da Conceição Patrícia Marluci , de Azevedo Fernando Alves
Published: August 2023

Root rot and gummosis caused by Phytophthora spp. are considered one of the most important citrus diseases in Brazil. Symptoms of Phytophthora spp. infection in citrus plants are associated with lesions on the bark at the base of the plant, roots, and even lower branches. Inappropriately performed cultural practices, such as excessive irrigation and practices that result in reduced aeration and soil drainage, favor the disease. The objective of this study was to evaluate the effect of Urochloa ruziziensis mulching provided by an ecological mower on the severity of Phytophthora nicotianae root rot and the development of citrus plants and their root system. The experiments were carried out under controlled conditions on Rangpur lime seedlings kept in pots containing soil from ‘Hamlin’ orange orchards with the use of an ecological mower or a conventional mower in the management of inter-row vegetation for six years. Urochloa ruziziensis mulching (9 t ha−1) was added or not to the pots. The inoculation of P. nicotianae in the roots occurred through infection of the soil with a solution containing mycelium and sporangia of the pathogen. A 2 × 2 × 2 factorial scheme was used, with the following factors A: soil type, B: inoculation of P. nicotianae and C: mulching of U. ruziziensis. The attributes of plant development, such as root growth, average number of leaves, leaves and dry mass of shoots and roots, in addition to assessments of the severity of disease in the roots, were evaluated. The mulch of U. ruziziensis did not promote an increase in root rot. The soil resulting from this management promoted the better development of Rangpur lime plants. Therefore, vegetation management of inter-row with an ecological mower is not associated with increased symptoms of Phytophthora root rot.
Determining the Relationship between Aroma and Quality of Bao-Chung Tea by Solid-Phase Microextraction (SPME) and Electronic Nose Analyses
Authors: Chen Po-An , Liu Chieh-I , Chen Kuo-Renn
Published: August 2023

Despite extensive studies, the relationship between the quality/quantity of tea odorants and oolong tea quality remains unclear. To investigate the key components affecting Bao-chung tea quality, we collected samples of different grades from a tea-tasting competition and determined the content and composition of volatile components and individual catechins using gas chromatography–mass spectrometry and high-performance liquid chromatography. We used an electronic nose (E-nose) to collect odor component signals and established a quality recognition model. The different tea grades did not significantly differ in catechin content, but their specific odor intensity and proportion of odor components varied significantly. Linear discriminant analysis showed that the intensity and proportion of volatile organic compounds could be used for distinguishing the different grades of Bao-chung tea. By combining different quantities of indole, linalool, and butanoic acid and proportions of p-cymene, cis-β-ocimene, nonanal, allo-ocimene, cis-jasmone, and α-farnesene, the ability to distinguish among Bao-chung tea grades was significantly improved. Our results revealed that the quality of Bao-chung tea should be evaluated based on the combined perception of odor component intensity and proportion rather than solely relying on the concentration or composition of specific compounds. Therefore, individuals can judge a Bao-chung tea grade based on the combined perception of odor component intensity and proportion. The E-nose can be used to identify Bao-chung tea grades based on its ability to determine the odorant composition.
Biofilm and Cancer: Interactions and Future Directions for Cancer Therapy
Authors: Choi Euna , Murray Ben , Choi Sunga
Published: August 2023

There is a growing body of evidence supporting the significant role of bacterial biofilms in the pathogenesis of various human diseases, including cancer. Biofilms are polymicrobial communities enclosed within an extracellular matrix composed of polysaccharides, proteins, extracellular DNA, and lipids. This complex matrix provides protection against antibiotics and host immune responses, enabling the microorganisms to establish persistent infections. Moreover, biofilms induce anti-inflammatory responses and metabolic changes in the host, further facilitating their survival. Many of these changes are comparable to those observed in cancer cells. This review will cover recent research on the role of bacterial biofilms in carcinogenesis, especially in colorectal (CRC) and gastric cancers, emphasizing the shared physical and chemical characteristics of biofilms and cancer. This review will also discuss the interactions between bacteria and the tumor microenvironment, which can facilitate oncogene expression and cancer progression. This information will provide insight into developing new therapies to identify and treat biofilm-associated cancers, such as utilizing bacteria as delivery vectors, using bacteria to upregulate immune function, or more selectively targeting biofilms and cancer for their shared traits.
Elexacaftor Mediates the Rescue of F508del CFTR Functional Expression Interacting with MSD2
Authors: Bongiorno Roberta , Ludovico Alessandra , Moran Oscar , Baroni Debora
Published: August 2023

Cystic fibrosis (CF) is one of the most frequent lethal autosomal recessive diseases affecting the Caucasian population. It is caused by loss of function variants of the cystic fibrosis transmembrane conductance regulator (CFTR), a membrane protein located on the apical side of epithelial cells. The most prevalent CF-causing mutation, the deletion of phenylalanine at position 508 (F508del), is characterized by folding and trafficking defects, resulting in the decreased functional expression of the protein on the plasma membrane. Two classes of small-molecule modulators, termed potentiators and correctors, respectively, have been developed to rescue either the gating or the cellular processing of defective F508del CFTR. Kaftrio, a next-generation triple-combination drug, consisting of the potentiator ivacaftor (VX770) and the two correctors tezacaftor (VX661) and elexacaftor (VX445), has been demonstrated to be a life-changing therapeutic modality for the majority of people with CF worldwide. While the mechanism of action of VX770 and VX661 is almost known, the precise mechanism of action and binding site of VX445 have not been conclusively determined. We investigated the activity of VX445 on mutant F508del to identify the protein domains whose expression is mostly affected by this corrector and to disclose its mechanisms of action. Our biochemical analyses revealed that VX445 specifically improves the expression and the maturation of MSD2, heterologously expressed in HEK 293 cells, and confirmed that its effect on the functional expression of defective F508del CFTR is additive either with type I or type II CFTR correctors. We are confident that our study will help to make a step forward in the comprehension of the etiopathology of the CF disease, as well as to give new information for the development and testing of combinations of even more effective correctors able to target mutation-specific defects of the CFTR protein.
Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites
Authors: Horvath Istvan , Mohamed Khadra A. , Kumar Ranjeet , Wittung-Stafshede Pernilla
Published: August 2023

The assembly of α-synuclein into cross-β structured amyloid fibers results in Lewy body deposits and neuronal degeneration in Parkinson’s disease patients. As the cell environment is highly crowded, interactions between the formed amyloid fibers and a range of biomolecules can occur in cells. Although amyloid fibers are considered chemically inert species, recent in vitro work using model substrates has shown α-synuclein amyloids, but not monomers, to catalyze the hydrolysis of ester and phosphoester bonds. To search for putative catalytic activity of α-synuclein amyloids on biologically relevant metabolites, we here incubated α-synuclein amyloids with neuronal SH-SY5Y cell lysates devoid of proteins. LC-MS-based metabolomic (principal component and univariate) analysis unraveled distinct changes in several metabolite levels upon amyloid (but not monomer) incubation. Of 63 metabolites identified, the amounts of four increased (3-hydroxycapric acid, 2-pyrocatechuic acid, adenosine, and NAD), and the amounts of seventeen decreased (including aromatic and apolar amino acids, metabolites in the TCA cycle, keto acids) in the presence of α-synuclein amyloids. Many of these metabolite changes match what has been reported previously in Parkinson’s disease patients and animal–model metabolomics studies. Chemical reactivity of α-synuclein amyloids may be a new gain-of-function that alters the metabolite composition in cells and, thereby, modulates disease progression.
Special Issue “Stem Cell Biology & Regenerative Medicine”
Authors: Ofir Rivka
Published: August 2023

More than 50% of pre-clinical studies fail despite a long and expensive journey of drug discovery using animal models [...]
Tic-Tac: A Translational Approach in Mechanisms Associated with Irregular Heartbeat and Sinus Rhythm Restoration in Atrial Fibrillation Patients
Authors: Parra-Lucares Alfredo , Villa Eduardo , Romero-Hernández Esteban , Méndez-Valdés Gabriel , Retamal Catalina , Vizcarra Geovana , Henríquez Ignacio , Maldonado-Morales Esteban A. J. , Grant-Palza Juan H. , Ruíz-Tagle Sofía , Estrada-Bobadilla Victoria , Toro Luis
Published: August 2023

Atrial fibrillation (AF) is a prevalent cardiac condition predominantly affecting older adults, characterized by irregular heartbeat rhythm. The condition often leads to significant disability and increased mortality rates. Traditionally, two therapeutic strategies have been employed for its treatment: heart rate control and rhythm control. Recent clinical studies have emphasized the critical role of early restoration of sinus rhythm in improving patient outcomes. The persistence of the irregular rhythm allows for the progression and structural remodeling of the atria, eventually leading to irreversible stages, as observed clinically when AF becomes permanent. Cardioversion to sinus rhythm alters this progression pattern through mechanisms that are still being studied. In this review, we provide an in-depth analysis of the pathophysiological mechanisms responsible for maintaining AF and how they are modified during sinus rhythm restoration using existing therapeutic strategies at different stages of clinical investigation. Moreover, we explore potential future therapeutic approaches, including the promising prospect of gene therapy.
The Intricate Role of Ecdysis Triggering Hormone Signaling in Insect Development and Reproductive Regulation
Authors: Malhotra Pooja , Basu Saumik
Published: August 2023

Insect growth is interrupted by molts, during which the insect develops a new exoskeleton. The exoskeleton confers protection and undergoes shedding between each developmental stage through an evolutionarily conserved and ordered sequence of behaviors, collectively referred to as ecdysis. Ecdysis is triggered by Ecdysis triggering hormone (ETH) synthesized and secreted from peripheral Inka cells on the tracheal surface and plays a vital role in the orchestration of ecdysis in insects and possibly in other arthropod species. ETH synthesized by Inka cells then binds to ETH receptor (ETHR) present on the peptidergic neurons in the central nervous system (CNS) to facilitate synthesis of various other neuropeptides involved in ecdysis. The mechanism of ETH function on ecdysis has been well investigated in holometabolous insects such as moths Manduca sexta and Bombyx mori, fruit fly Drosophila melanogaster, the yellow fever mosquito Aedes aegypti and beetle Tribolium castaneum etc. In contrast, very little information is available about the role of ETH in sequential and gradual growth and developmental changes associated with ecdysis in hemimetabolous insects. Recent studies have identified ETH precursors and characterized functional and biochemical features of ETH and ETHR in a hemimetabolous insect, desert locust, Schistocerca gregaria. Recently, the role of ETH in Juvenile hormone (JH) mediated courtship short-term memory (STM) retention and long-term courtship memory regulation and retention have also been investigated in adult male Drosophila. Our review provides a novel synthesis of ETH signaling cascades and responses in various insects triggering diverse functions in adults and juvenile insects including their development and reproductive regulation and might allow researchers to develop sustainable pest management strategies by identifying novel compounds and targets.
Influence of Antiphospholipid Antibody-Associated Thrombophilia on the Risk of Preterm Birth: A Systematic Review
Authors: Iordache Olivera , Anastasiu Doru Mihai , Kakarla Manaswini , Ali Ayesha , Bratosin Felix , Neamtu Radu , Dumitru Catalin , Olaru Flavius , Erdelean Izabella , Gherman Angelica , Avram Cecilia Roberta , Stelea Lavinia
Published: August 2023

Antiphospholipid antibody (aPL)-associated thrombophilia has been implicated in various adverse pregnancy outcomes, including preterm birth and impaired fetal development. This systematic review aimed to elucidate the relationship between aPL-associated thrombophilia and these outcomes, as well as to identify potential modifiers of this relationship such as maternal age, coexisting maternal medical conditions, type of aPL antibodies involved, and the timing of thrombophilia diagnosis during gestation. We conducted a comprehensive literature search in PubMed, Web of Science, Cochrane, and Scopus in May 2023, covering literature published within the last 10 years. Eight articles, involving 2935 patients, were eligible for inclusion in the review. Single aCL was the most common type of aPL found in patients, with rates up to 61.0% in some studies, followed by single LA and single ab2GPI. Multiple aPL antibody positivity was found to be associated with a higher risk of preterm birth, with odds ratios ranging from 1.29 to 9.61. Patient characteristics and previous pregnancy history varied significantly across the studies. Risk factors such as diabetes mellitus, thrombosis, and systemic lupus erythematosus were also variable across the studies, but presence of these risk factors did not consistently affect the risk of preterm birth. Furthermore, although a triple positive aPL test was the most important risk factor for preterm birth, it was observed that thrombophilia treatment during pregnancy significantly reduced the risk by 2.44 times (95% CI = 1.18–6.20). This review supports the evidence for aPL-associated thrombophilia being a significant contributor to preterm birth and fetal developmental abnormalities. Further research is required to investigate the exact mechanisms and to determine the best clinical management for patients with aPL-associated thrombophilia during pregnancy.
Predictors of Extraprostatic Extension in Patients with Prostate Cancer
Authors: Kim See Hyung , Cho Seung Hyun , Kim Won Hwa , Kim Hye Jung , Park Jong Min , Kim Gab Chul , Ryeom Hun Kyu , Yoon Yu Sung , Cha Jung Guen
Published: August 2023

Purpose: To identify effective factors predicting extraprostatic extension (EPE) in patients with prostate cancer (PCa). Methods: This retrospective cohort study recruited 898 consecutive patients with PCa treated with robot-assisted laparoscopic radical prostatectomy. The patients were divided into EPE and non-EPE groups based on the analysis of whole-mount histopathologic sections. Histopathological analysis (ISUP biopsy grade group) and magnetic resonance imaging (MRI) (PI-RADS v2.1 scores [1–5] and the Mehralivand EPE grade [0–3]) were used to assess the prediction of EPE. We also assessed the clinical usefulness of the prediction model based on decision-curve analysis. Results: Of 800 included patients, 235 (29.3%) had EPE, and 565 patients (70.7%) did not (non-EPE). Multivariable logistic regression analysis showed that the biopsy ISUP grade, PI-RADS v2.1 score, and Mehralivand EPE grade were independent risk factors for EPE. In the regression assessment of the models, the best discrimination (area under the curve of 0.879) was obtained using the basic model (age, serum PSA, prostate volume at MRI, positive biopsy core, clinical T stage, and D’Amico risk group) and Mehralivand EPE grade 3. Decision-curve analysis showed that combining Mehralivand EPE grade 3 with the basic model resulted in superior net benefits for predicting EPE. Conclusion: Mehralivand EPE grades and PI-RADS v2.1 scores, in addition to basic clinical and demographic information, are potentially useful for predicting EPE in patients with PCa.
Sex Differences in Prognosis of Heart Failure Due to Ischemic and Nonischemic Cardiomyopathy
Authors: Mansur Antonio de Padua , Pereira-Barretto Antonio Carlos , del Carlo Carlos Henrique , Avakian Solange Desirée , Nakagawa Naomi Kondo , Cesar Luiz Antonio Machado , Bocchi Edimar Alcides
Published: August 2023

Background: Limited research has explored sex-specific differences in death predictors of HF patients with ischemic (iCMP) and nonischemic (niCMP) cardiomyopathy. This study assessed sex differences in niCMP and iCMP prognosis. Methods: We studied 7487 patients with HF between February 2017 and September 2020. Clinical features and echocardiographic findings were collected. We used Kaplan–Meier, Cox proportional hazard models, and chi-square scores of Cox regression to determine death predictors in women and men. Results: The mean age was 64.3 ± 14.2 years, with 4417 (59%) males. Women with iCMP and niCMP exhibited a significantly higher mean age, higher mean left ventricular ejection fraction, and smaller left ventricular diastolic diameter than men. Over 2.26 years of follow-up, 325 (14.7%) women and 420 (15.7%) men, and 211 women (24.5%) and 519 men (29.8%) with niCMP (p = NS) and iCMP (p = 0.004), respectively, died. The cumulative incidence of death was higher in men with iCMP (log-rank p < 0.0001) but similar with niCMP. Cox regression showed chronic kidney disease, diabetes, stroke, atrial fibrillation, age, and myocardial infarction as the main predictors of death for iCMP in women and men. Conclusions: Women exhibited a better prognosis than men with iCMP, but similar for niCMP. Nevertheless, sex was not an independent predictor of death for both CMP.
Association of TNF-α (-308G/A) Gene Polymorphism with Changes in Circulating TNF-α Levels in Response to CPAP Treatment in Adults with Coronary Artery Disease and Obstructive Sleep Apnea
Authors: Celik Yeliz , Peker Yüksel , Yucel-Lindberg Tülay , Thelander Tilia , Behboudi Afrouz
Published: August 2023

Rationale: We recently demonstrated that patients with coronary artery disease (CAD) and obstructive sleep apnea (OSA) carrying the tumor necrosis factor-alpha (TNF-α) A allele had increased circulating TNF-α levels compared with the ones carrying the TNF-α G allele. In the current study, we addressed the effect of TNF-α (-308G/A) gene polymorphism on circulating TNF-α levels following continuous positive airway pressure (CPAP) therapy. Methods: This study was a secondary analysis of the RICCADSA trial (NCT00519597) conducted in Sweden. CAD patients with OSA (apnea–hypopnea index) of ≥15 events/h and an Epworth Sleepiness Scale (ESS) score of <10 were randomized to CPAP or no-CPAP groups, and OSA patients with an ESS score of ≥10 were offered CPAP treatment. Blood samples were obtained at baseline and 12-month follow-up visits. TNF-α was measured by immunoassay (Luminex, R&D Systems). Genotyping of TNF-α-308G/A (single nucleotide polymorphism Rs1800629) was performed by polymerase chain reaction–restriction fragment length polymorphism. Results: In all, 239 participants (206 men and 33 women; mean age 64.9 (SD 7.7) years) with polymorphism data and circulating levels of TNF-α at baseline and 1-year follow-up visits were included. The median circulating TNF-α values fell in both groups between baseline and 12 months with no significant within- or between-group differences. In a multivariate linear regression model, a significant change in circulating TNF-α levels from baseline across the genotypes from GA to GA and GA to AA (standardized β-coefficient −0.129, 95% confidence interval (CI) −1.82; −0.12; p = 0.025) was observed in the entire cohort. The association was more pronounced among the individuals who were using the device for at least 4 h/night (n = 86; standardized β-coefficient −2.979 (95% CI −6.11; −1.21); p = 0.004)), whereas no significant association was found among the patients who were non-adherent or randomized to no-CPAP. The participants carrying the TNF-α A allele were less responsive to CPAP treatment regarding the decline in circulating TNF-α despite CPAP adherence (standardized β-coefficient −0.212, (95% CI −5.66; −1.01); p = 0.005). Conclusions: Our results suggest that TNF-α (-308G/A) gene polymorphism is associated with changes in circulating TNF-α levels in response to CPAP treatment in adults with CAD and OSA.