bluuebunny/crossref_metadata_2025 huggingface dataset:
Sedation Rate Reduction in Paediatric Renal Nuclear Medicine Examinations: Consequences of a Targeted Audit
Authors: Gernhold Christa , Kundtner Nina , Steinmair Martin , Henkel Martin , Oswald Josef , Haid Bernhard
Published: May 2021

Background: Nuclear medicine investigations are essential diagnostic tools in paediatric urology. Child-orientated examination techniques and the avoidance of sedation or anaesthesia vary in different institutions. We aimed at evaluating child friendly measures in our department to identify the potential for improvement. Based on these data, we changed the standards regarding the sedation policy and consequently re-evaluated sedation rates. Methods: Four-hundred thirty-five consecutive investigations were evaluated regarding the need for sedation, outcome and patient satisfaction at our department. After the revision of our department standards, we re-evaluated 159 examinations. Statistical analysis was performed with JUMBO (Java-supported Münsterian biometrical platform). Results: Eighty-six percent (60/70) would agree to perform an investigation under identical conditions again. Seventy-seven percent (17/22) of eligible patients >5 years of age felt good during the investigation. By changing our sedation policy, we could reduce the sedation rate from 27.1% to 7.5% (p < 0.0001; OR 0.219 95% CI 0.111–0.423). Conclusion: The evaluation of child friendly examination protocols demonstrated high reliability and patient satisfaction using situational sedation with a relatively high proportion of patients being sedated. Through protocol adaption with clear age limits, individual indication and education of staff, as well as the use of optimized sedatives, the need for sedation could be further reduced whilst maintaining a high patient satisfaction.
Maternal Blood Group and Routine Direct Antiglobulin Testing in Neonates: Is There a Role for Selective Neonatal Testing?
Authors: Shash Hwazen A. , Alkhater Suzan A.
Published: May 2021

Recommendations for the screening of hemolytic disease of the newborn (HDN) advise taking a selective approach in using the direct antiglobulin test (DAT) for mothers with blood group O or RhD-negative. This study assessed the relation of DAT results to maternal and neonatal blood groups and evaluated the risk of HDN. A retrospective analysis of all healthy newborns admitted during 2018 was performed. Of 1463 newborns, 4.4% had a positive DAT. There were 541 (37%) maternal–neonatal pairs with ABO incompatibility, most commonly born to mothers with blood group O. The cohort of neonates born to mothers with blood group O was divided into three groups: the O-A and O-B groups and the O-O group as a control. The DAT was positive in 59 (8.3%) neonates; most were in the O-B group (49.2%), whereas 13.6% were in the control group (p < 0.01). While the neonates in the O-B group were more likely to require phototherapy (p = 0.03), this finding was not related to DAT results. We found that selective testing of mothers with blood group O, mothers with blood group O or RhD-negative, neonates with blood group B, and neonates with blood group B born to mothers with blood group O or RhD-negative was ineffective in detecting phototherapy requirements. Our results indicate no difference regarding the need for phototherapy in neonates born to mothers with different blood types regardless of the DAT results.
Fabrication of Thermal Plasma Sprayed NiTi Coatings Possessing Functional Properties
Authors: Samal Sneha , Tyc Ondřej , Cizek Jan , Klecka Jakub , Lukáč František , Molnárová Orsolya , de Prado Esther , Weiss Zdeněk , Kopeček Jaromír , Heller Luděk , Šittner Petr , Chráska Tomáš
Published: May 2021

Thick NiTi shape memory alloy coatings (300–500 µm) were produced on graphite and AISI 304 substrates by radio frequency inductively-coupled plasma spray technology (RF-ICP) from feedstock NiTi powders. Their microstructure as well as chemical and phase composition were characterized and a methodology for the characterization of functional shape memory properties of the thick coatings was developed. The coatings exhibited cubic to monoclinic martensitic transformation and shape memory effect. The presented results prove that NiTi coatings with functional thermomechanical properties can be easily produced on structural materials by RF-ICP. Further optimization will be needed to prepare NiTi coatings with better microstructural and chemical homogeneity.
Editorial of Special Issue “Differential Diagnosis for Dry Eye”
Authors: Georgiev Georgi As. , Yokoi Norihiko
Published: May 2021

This editorial aims to summarize the scientific papers that contributed to the Special Issue “Differential Diagnosis for Dry Eye” [...]
On the Political Decision of Audit Market Regulation: Empirical Evidence of Audit Firm Tenure and Maximum Durations within the European Union
Authors: Widmann Markus , Follert Florian , Wolz Matthias
Published: May 2021

After almost every economic crisis and corporate scandal, political actors announce the need for stricter regulatory measures for financial markets and companies, in an attempt to appease their voters and defend their political agenda. Regarding the latest international financial crisis, the EU responded, among other things, with comprehensive regulation of the European audit market. In the context of auditor rotation, after 17 June 2016, the duration of audit engagements should not exceed a maximum of 10 years. In this paper, we therefore investigate whether there is empirical evidence behind the 10-year threshold—or is it simply arbitrary? Our aim is to evaluate the audit market reform by the European Union (EU) (Regulation (EU) No 537/2014 and Directive, 2014/56/EU) related to the objective of improving the quality of audits. Therefore, our article addresses the most crucial element of this reform, the implementation of a mandatory audit firm rotation for public interest entities (Regulation (EU) No 537/2014, Article 17). Based on a unique dataset of 11,834 firm observations from all listed companies within the EU between 2008 and 2017, we provide for the first time a discussion basis for the assessment of audit market interventions by the EU. Hence, we compare the new maximum durations with average audit tenure in the particular member states. Even where we present only descriptive results, our results at least indicate that the “magic number” 10 (years) could be more the result of a political process—i.e., a consent between the European institutions—rather than evidence based. Therefore, our findings shall serve as a first starting point in the discussion of a vast and interdisciplinary research field.
Classification of Tree Functional Types in a Megadiverse Tropical Mountain Forest from Leaf Optical Metrics and Functional Traits for Two Related Ecosystem Functions
Authors: Limberger Oliver , Homeier Jürgen , Farwig Nina , Pucha-Cofrep Franz , Fries Andreas , Leuschner Christoph , Trachte Katja , Bendix Jörg
Published: May 2021

Few plant functional types (PFTs) with fixed average traits are used in land surface models (LSMs) to consider feedback between vegetation and the changing atmosphere. It is uncertain if highly diverse vegetation requires more local PFTs. Here, we analyzed how 52 tree species of a megadiverse mountain rain forest separate into local tree functional types (TFTs) for two functions: biomass production and solar radiation partitioning. We derived optical trait indicators (OTIs) by relating leaf optical metrics and functional traits through factor analysis. We distinguished four OTIs explaining 38%, 21%, 15%, and 12% of the variance, of which two were considered important for biomass production and four for solar radiation partitioning. The clustering of species-specific OTI values resulted in seven and eight TFTs for the two functions, respectively. The first TFT ensemble (P-TFTs) represented a transition from low to high productive types. The P-TFT were separated with a fair average silhouette width of 0.41 and differed markedly in their main trait related to productivity, Specific Leaf Area (SLA), in a range between 43.6 to 128.2 (cm2/g). The second delineates low and high reflective types (E-TFTs), were subdivided by different levels of visible (VIS) and near-infrared (NIR) albedo. The E-TFTs were separated with an average silhouette width of 0.28 and primarily defined by their VIS/NIR albedo. The eight TFT revealed an especially pronounced range in NIR reflectance of 5.9% (VIS 2.8%), which is important for ecosystem radiation partitioning. Both TFT sets were grouped along elevation, modified by local edaphic gradients and species-specific traits. The VIS and NIR albedo were related to altitude and structural leaf traits (SLA), with NIR albedo showing more complex associations with biochemical traits and leaf water. The TFTs will support LSM simulations used to analyze the functioning of mountain rainforests under climate change.
Genetics and Epigenetics of One-Carbon Metabolism Pathway in Autism Spectrum Disorder: A Sex-Specific Brain Epigenome?
Authors: Tisato Veronica , Silva Juliana A. , Longo Giovanna , Gallo Ines , Singh Ajay V. , Milani Daniela , Gemmati Donato
Published: May 2021

Autism spectrum disorder (ASD) is a complex neurodevelopmental condition affecting behavior and communication, presenting with extremely different clinical phenotypes and features. ASD etiology is composite and multifaceted with several causes and risk factors responsible for different individual disease pathophysiological processes and clinical phenotypes. From a genetic and epigenetic side, several candidate genes have been reported as potentially linked to ASD, which can be detected in about 10–25% of patients. Folate gene polymorphisms have been previously associated with other psychiatric and neurodegenerative diseases, mainly focused on gene variants in the DHFR gene (5q14.1; rs70991108, 19bp ins/del), MTHFR gene (1p36.22; rs1801133, C677T and rs1801131, A1298C), and CBS gene (21q22.3; rs876657421, 844ins68). Of note, their roles have been scarcely investigated from a sex/gender viewpoint, though ASD is characterized by a strong sex gap in onset-risk and progression. The aim of the present review is to point out the molecular mechanisms related to intracellular folate recycling affecting in turn remethylation and transsulfuration pathways having potential effects on ASD. Brain epigenome during fetal life necessarily reflects the sex-dependent different imprint of the genome-environment interactions which effects are difficult to decrypt. We here will focus on the DHFR, MTHFR and CBS gene-triad by dissecting their roles in a sex-oriented view, primarily to bring new perspectives in ASD epigenetics.
Erratum: Merisalu et al. A Framework for Risk-Based Cost–Benefit Analysis for Decision Support on Hydrogeological Risks in Underground Construction. Geosciences 2021, 11, 82
Authors:   Geosciences Editorial Office
Published: May 2021

The Geosciences Editorial Office would like to make the following change to this paper [...]
Morphometric Analysis of Coptotermes spp. Soldier Caste (Blattodea: Rhinotermitidae) in Indonesia and Evidence of Coptotermes gestroi Extreme Head-Capsule Shapes
Authors: Wikantyoso Bramantyo , Tseng Shu-Ping , Himmi Setiawan Khoirul , Yusuf Sulaeman , Yoshimura Tsuyoshi
Published: May 2021

Linear and geometric morphometrics approaches were conducted to analyze the head capsule (HC) shape of collected soldier caste specimens of Coptotermes from various locations in Indonesia. The soldiers’ morphology was observed and measured. The results of the principal component analysis of the group of all species showed two important groups of variables, i.e., the body size and setae characteristics of the pronotum and head. The multicollinearity of the morphometric variables showed the importance of body measurements as well as important alternative characteristics such as the pronotum setae (PrS) and HC setae. Four trends of HC shape were observed across the species. Interestingly, three extreme shapes were depicted by geometric morphometrics of the C. gestroi HC. The phylogenetic tree inferred from 12S and 16S mitochondrial gene fragments showed high confidence for C. gestroi populations. The lateral expansion of the posterior part of the HC across the species was in accordance with the increasing of the number of hairlike setae on the pronotum and HC. These differences among species might be associated with mandible-force-related defensive labor and sensitivity to environmental stressors.
A Portable Low-Cost Ultrasound Measurement Device for Concrete Monitoring
Authors: Fontoura Barroso Daniel , Epple Niklas , Niederleithinger Ernst
Published: May 2021

This paper describes a new ultrasonic measuring device called “W-Box”. It was developed based on the requirements of the DFG Forschergruppe (research unit) CoDA for a portable device for monitoring of concrete specimens, models and actual structures using embedded ultrasonic transducers as well as temperature and humidity sensors. The W-Box can send ultrasonic pulses with a variable frequency of 50–100 kHz to one selectable transducer and records signals from up to 75 multiplexed channels with a sample rate of 1 MHz and a resolution of 14 bits. In addition, it measures temperature and humidity with high accuracy, adjustable amplification, restarts automatically after a power failure and can be fully controlled remotely. The measured data are automatically stored locally on-site data quality checks and transferred to remote servers. The comparison of the W-Box with a laboratory setup using commercial devices proves that it is equally reliable and precise, at much lower cost. The W-Box also shows that their measurement capacities, with the used embedded ultrasonic transducers, can reach above 6 m in concrete.
Reliability Modelling and Analysis of the Power Take-Off System of an Oscillating Wave Surge Converter
Authors: Heikkilä Eetu , Välisalo Tero , Tiusanen Risto , Sarsama Janne , Räikkönen Minna
Published: May 2021

Wave power is a potential technology for generating sustainable renewable energy. Several types of wave energy converters (WECs) have been proposed for this purpose. WECs operate in a harsh maritime environment that sets strict limitations on how and when the device can be economically and safely reached for maintenance. Thus, to ensure profitable energy generation over the system life cycle, system reliability is a key aspect to be considered in WEC development. In this article, we describe a reliability analysis approach for WEC development, based on the use of reliability block diagram (RBD) modelling. We apply the approach in a case study involving a submerged oscillating wave surge converter device concept that utilizes hydraulics in its power take-off system. In addition to describing the modelling approach, we discuss the data sources that were used for gathering reliability data for the components used in a novel system concept with very limited historical or experimental data available. This includes considerations of the data quality from various sources. As a result, we present examples of applying the RBD modelling approach in the context of WEC development and discuss the applicability of the approach in supporting the development of new technologies.
Upper Pleistocene and Holocene Storm Deposits Eroded from the Granodiorite Coast on Isla San Diego (Baja California Sur, Mexico)
Authors: Callahan Ginni , Johnson Markes E. , Guardado-France Rigoberto , Ledesma-Vázquez Jorge
Published: May 2021

This project examines the role of hurricane-strength events likely to have exceeded 119 km/h in wind speed that entered the Gulf of California from the open Pacific Ocean during Late Pleistocene and Holocene times to impact the granodiorite shoreline on Isla San Diego. Conglomerate dominated by large, ellipsoidal to subspherical boulders at the islands south end were canvassed at six stations. A total of 200 individual cobbles and boulders were systematically measured in three dimensions, providing the database for analyses of variations in clast shape and size. The project’s goal was to apply mathematical equations elaborated after Nott (2003) with subsequent refinements to estimate individual wave heights necessary to lift igneous blocks from the joint-bound and exfoliated coast on Isla San Diego. On average, wave heights on the order of 3 m are calculated as having impacted the Late Pleistocene rocky coastline on Isla San Diego during storms, although the largest boulders more than a meter in diameter are estimated to weigh two metric tons and would have required waves in excess of 10 m for extraction. Described for the first time, a fossil marine biota associated with the boulder beds confirms a littoral-to-very-shallow water setting correlated with Marine Isotope Substage 5e approximately 125,000 years ago. A narrow submarine ridge consisting, in part, of loose cobbles and boulders extends for 1.4 km to the southwest from the island’s tip, suggesting that Holocene storms continued to transport rock debris removed from the shore. The historical record of events registered on the Saffir–Simpson Hurricane Wind Scale in the Gulf of California suggests that major storms with the same intensity struck the island in earlier times.
Two-Dimensional Nitronyl Nitroxide–Cu Networks Based on Multi-Dentate Nitronyl Nitroxides: Structures and Magnetic Properties
Authors: Li Hongdao , Lu Jiao , Xie Jing , Jing Pei , Li Licun
Published: May 2021

Two multi-dentate nitronyl nitroxide radicals, namely, bisNITPhPy ([5-(4-pyridyl)-1,3-bis(1′-oxyl-3′-oxido-4′,4′,5′,5′-tetramethyl-4,5-hydro-1H-imidazol-2-yl)]benzene) and NIT-3Py-5-4Py (2-{3-[5-(4-pyridyl)]pyridyl}-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide), were assembled with CuII ions to obtain two-dimensional heterospin 2p–3d coordination polymers [Cu7(hfac)14(bisNITPhPy)2]n (1) and [Cu2(hfac)4(NIT-3Py-5-4Py)]n (2) (hfac: hexafluoroacetylacetonate). In both compounds, the bisNITPhPy and NIT-3Py-5-3Py radicals acted as pentadentate and tetradentate ligands, respectively, to connect with CuII ions, generating a 2D layer structure. The analysis of the magnetic behavior indicated that strong antiferromagnetic coupling and ferromagnetic interaction (J = 17.1 cm−1) coexisted in 1. For 2, there were ferromagnetic couplings between the CuII ion and NO group, as well as the CuII ion and radical via the pyridine ring with J1 = 32.8 and J2 = 2.2 cm−1, respectively.
Analytical Solutions of the Fractional Mathematical Model for the Concentration of Tumor Cells for Constant Killing Rate
Authors: Ahmed Najma , Shah Nehad Ali , Ali Farman , Vieru Dumitru , Zaman F.D.
Published: May 2021

Two generalized mathematical models with memory for the concentration of tumor cells have been analytically studied using the cylindrical coordinate and the integral transform methods. The generalization consists of the formulating of two mathematical models with Caputo-time fractional derivative, models that are suitable to highlight the influence of the history of tumor evolution on the present behavior of the concentration of cancer cells. The time-oscillating concentration of cancer cells has been considered on the boundary of the domain. Analytical solutions of the fractional differential equations of the mathematical models have been determined using the Laplace transform with respect to the time variable and the finite Hankel transform with respect to the radial coordinate. The positive roots of the transcendental equation with Bessel function J0(r)=0, which are needed in our study, have been determined with the subroutine rn=root(J0(r),r,(2n−1)π/4,(2n+3)π/4),n=1,2,… of the Mathcad 15 software. It is found that the memory effects are stronger at small values of the time, t. This aspect is highlighted in the graphical illustrations that analyze the behavior of the concentration of tumor cells. Additionally, the concentration of cancer cells is symmetric with respect to radial angle, and its values tend to be zero for large values of the time, t.
Profitability Index Maximization in an Inventory Model with a Price- and Stock-Dependent Demand Rate in a Power-Form
Authors: Pando Valentín , San-José Luis A. , Sicilia Joaquín , Alcaide-López-de-Pablo David
Published: May 2021

This paper presents the optimal policy for an inventory model where the demand rate potentially depends on both selling price and stock level. The goal is the maximization of the profitability index, defined as the ratio income/expense. A numerical algorithm is proposed to calculate the optimal selling price. The optimal values for the depletion time, the cycle time, the maximum profitability index, and the lot size are evaluated from the selling price. The solution shows that the inventory must be replenished when the stock is depleted, i.e., the depletion time is always equal to the cycle time. The optimal policy is obtained with a suitable balance between ordering cost and holding cost. A condition that ensures the profitability of the financial investment in the inventory is established from the initial parameters. Profitability thresholds for several parameters, including the scale and the non-centrality parameters, keeping all the others fixed, are evaluated. The model with an isoelastic price-dependent demand is solved as a particular case. In this last model, all the optimal values are given in a closed form, and a sensitivity analysis is performed for several parameters, including the scale parameter. The results are illustrated with numerical examples.
Microstructured Hollow Fiber Membranes: Potential Fiber Shapes for Extracorporeal Membrane Oxygenators
Authors: Ecker Paul , Pekovits Markus , Yorov Tsvetan , Haddadi Bahram , Lukitsch Benjamin , Elenkov Martin , Janeczek Christoph , Jordan Christian , Gfoehler Margit , Harasek Michael
Published: May 2021

Extracorporeal membrane oxygenators are essential medical devices for the treatment of patients with respiratory failure. A promising approach to improve oxygenator performance is the use of microstructured hollow fiber membranes that increase the available gas exchange surface area. However, by altering the traditional circular fiber shape, the risk of low flow, stagnating zones that obstruct mass transfer and encourage thrombus formation, may increase. Finding an optimal fiber shape is therefore a significant task. In this study, experimentally validated computational fluid dynamics simulations were used to investigate transverse flow within fiber packings of circular and microstructured fiber geometries. A numerical model was applied to calculate the local Sherwood number on the membrane surface, allowing for qualitative comparison of gas exchange capacities in low-velocity areas caused by the microstructured geometries. These adverse flow structures lead to a tradeoff between increased surface area and mass transfer. Based on our simulations, we suggest an optimal fiber shape for further investigations that increases potential mass transfer by up to 48% in comparison to the traditional, circular hollow fiber shape.
Novel Program Scheme of Vertical NAND Flash Memory for Reduction of Z-Interference
Authors: Yi Su-in , Kim Jungsik
Published: May 2021

Minimizing the variation in threshold voltage (Vt) of programmed cells is required to the extreme level for realizing multi-level-cells; as many as even 5 bits per cell recently. In this work, a recent program scheme to write the cells from the top, for instance the 170th layer, to the bottom, the 1st layer, (T-B scheme) in vertical NAND (VNAND) Flash Memory, is investigated to minimize Vt variation by reducing Z-interference. With the aid of Technology Computer Aided Design (TCAD) the Z-Interference for T-B (84 mV) is found to be better than B-T (105 mV). Moreover, under scaled cell dimensions (e.g., Lg: 31→24 nm), the improvement becomes protruding (T-B: 126 mV and B-T: 162 mV), emphasizing the significance of the T-B program scheme for the next generation VNAND products with the higher bit density.
A Deep Look into the Microbiology and Chemistry of Froth Treatment Tailings: A Review
Authors: Van Dongen Angeline , Samad Abdul , Heshka Nicole E. , Rathie Kara , Martineau Christine , Bruant Guillaume , Degenhardt Dani
Published: May 2021

In Alberta’s Athabasca oil sands region (AOSR), over 1.25 billion m3 of tailings waste from the bitumen extraction process are stored in tailings ponds. Fugitive emissions associated with residual hydrocarbons in tailings ponds pose an environmental concern and include greenhouse gases (GHGs), reduced sulphur compounds (RSCs), and volatile organic compounds (VOCs). Froth treatment tailings (FTT) are a specific type of tailings waste stream from the bitumen froth treatment process that contains bioavailable diluent: either naphtha or paraffins. Tailings ponds that receive FTT are associated with the highest levels of biogenic gas production, as diverse microbial communities biodegrade the residual diluent. In this review, current literature regarding the composition, chemical analysis, and microbial degradation of FTT and its constituents is presented in order to provide a more complete understanding of the complex chemistry and biological processes related to fugitive emissions from tailings ponds receiving FTT. Characterizing the composition and biodegradation of FTT is important from an environmental perspective to better predict emissions from tailings ponds and guide tailings pond management decisions.
Effect of Carbon Sources in Carotenoid Production from Haloarcula sp. M1, Halolamina sp. M3 and Halorubrum sp. M5, Halophilic Archaea Isolated from Sonora Saltern, Mexico
Authors: Vázquez-Madrigal Ana Sofía , Barbachano-Torres Alejandra , Arellano-Plaza Melchor , Kirchmayr Manuel Reinhart , Finore Ilaria , Poli Annarita , Nicolaus Barbara , De la Torre Zavala Susana , Camacho-Ruiz Rosa María
Published: May 2021

The isolation and molecular and chemo-taxonomic identification of seventeen halophilic archaea from the Santa Bárbara saltern, Sonora, México, were performed. Eight strains were selected based on pigmentation. Molecular identification revealed that the strains belonged to the Haloarcula, Halolamina and Halorubrum genera. Neutral lipids (quinones) were identified in all strains. Glycolipid S-DGD was found only in Halolamina sp. strain M3; polar phospholipids 2,3-O-phytanyl-sn-glycerol-1-phosphoryl-3-sn-glycerol (PG), 2,3-di-O-phytanyl-sn-glycero-1-phospho-3′-sn-glycerol-1′-methyl phosphate (PGP-Me) and sodium salt 1-(3-sn-phosphatidyl)-rac-glycerol were found in all the strains; and one unidentified glyco-phospholipid in strains M1, M3 and M4. Strains M1, M3 and M5 were selected for further studies based on carotenoid production. The effect of glucose and succinic and glutamic acid on carotenoid production was assessed. In particular, carotenoid production and growth significantly improved in the presence of glucose in strains Haloarcula sp. M1 and Halorubrum sp. M5 but not in Halolamina sp. M3. Glutamic and succinic acid had no effect on carotenoid production, and even was negative for Halorubrum sp. M5. Growth was increased by glutamic and succinic acid on Haloarcula sp. M1 but not in the other strains. This work describes for first time the presence of halophilic archaea in the Santa Bárbara saltern and highlights the differences in the effect of carbon sources on the growth and carotenoid production of haloarchaea.
Akkermansia muciniphila, a New Generation of Beneficial Microbiota in Modulating Obesity: A Systematic Review
Authors: Abuqwider Jumana Nabil , Mauriello Gianluigi , Altamimi Mohammad
Published: May 2021

Obesity is a complex syndrome and is recognized as the ultimate pathway of many chronic diseases. Studies using Akkermansia muciniphila supplementation strategy have proved to be effective for the prevention and treatment of obesity and other metabolic disorders. Although there are studies that support the protective effect of this strategy, the effects on the prevention of obesity on humans are not clear yet and need more investigation. The aim of this study is to investigate the effect of A. muciniphila administration on modulating obesity. This systematic review was generated from articles published within the last 10 years. All articles were in English and included animal subjects. The review relied on the search engines Google Scholar, Pub Med, Web of Science and Medline using the following keywords: A. muciniphila, next-generation probiotic, new-generation probiotic, obesity, fat mass, body fat and lipid profile. The search has revealed 804 articles with relevant key words. After the exclusion of irrelevant articles, 10 studies were selected based on the criteria. These studies were randomized controlled trials that have shown that A. muciniphila modulates obesity by regulating metabolism and energy hemostasis and improving insulin sensitivity and glucose hemostasis. In addition, studies showed this microorganism enhances low grade inflammation by different mechanisms.
Unicellular versus Filamentous: The Glacial Alga Ancylonema alaskana comb. et stat. nov. and Its Ecophysiological Relatedness to Ancylonema nordenskioeldii (Zygnematophyceae, Streptophyta)
Authors: Procházková Lenka , Řezanka Tomáš , Nedbalová Linda , Remias Daniel
Published: May 2021

Melting polar and alpine ice surfaces frequently exhibit blooms of dark pigmented algae. These microbial extremophiles significantly reduce the surface albedo of glaciers, thus accelerating melt rates. However, the ecology, physiology and taxonomy of cryoflora are not yet fully understood. Here, a Swiss and an Austrian glacier dominated either by filamentous Ancylonema nordenskioeldii or unicellular Mesotaenium berggrenii var. alaskanum, were sampled. Molecular analysis showed that both species are closely related, sharing identical chloroplast morphologies (parietal-lobed for Ancylonema vs. axial plate-like for Mesotaenium sensu stricto), thus the unicellular species was renamed Ancylonema alaskana. Moreover, an ecophysiological comparison of the two species was performed: pulse–amplitude modulated (PAM) fluorometry confirmed that they have a high tolerance to elevated solar irradiation, the physiological light preferences reflected the conditions in the original habitat; nonetheless, A. nordenskioeldii was adapted to higher irradiances while the photosystems of A. alaskana were able to use efficiently low irradiances. Additionally, the main vacuolar polyphenol, which effectively shields the photosystems, was identical in both species. Also, about half of the cellular fatty acids were polyunsaturated, and the lipidome profiles dominated by triacylglycerols were very similar. The results indicate that A. alaskana is physiologically very similar and closely related but genetically distinct to A. nordenskioeldii.
Long-Term Arsenic Sequestration in Biogenic Pyrite from Contaminated Groundwater: Insights from Field and Laboratory Studies
Authors: Fischer Alicia , Saunders James , Speetjens Sara , Marks Justin , Redwine Jim , Rogers Stephanie R. , Ojeda Ann S. , Rahman Md Mahfujur , Billor Zeki M. , Lee Ming-Kuo
Published: May 2021

Pumping groundwater from arsenic (As)-contaminated aquifers exposes millions of people, especially those in developing countries, to high doses of the toxic contaminant. Previous studies have investigated cost-effective techniques to remove groundwater arsenic by stimulating sulfate-reducing bacteria (SRB) to form biogenic arsenian pyrite. This study intends to improve upon these past methods to demonstrate the effectiveness of SRB arsenic remediation at an industrial site in Florida. This study developed a ferrous sulfate and molasses mixture to sequester groundwater arsenic in arsenian pyrite over nine months. The optimal dosage of the remediating mixture consisted of 5 kg of ferrous sulfate, ~27 kg (60 lbs) of molasses, and ~1 kg (2 lbs) of fertilizer per 3785.4 L (1000 gallons) of water. The remediating mixture was injected into 11 wells hydrologically upgradient of the arsenic plume in an attempt to obtain full-scale remediation. Groundwater samples and precipitated biominerals were collected from June 2018 to March 2019. X-ray diffraction (XRD), X-ray fluorescence (XRF), electron microprobe (EMP), and scanning electron microscope (SEM) analyses determined that As has been sequestered mainly in the form of arsenian pyrite, which rapidly precipitated as euhedral crystals and spherical aggregates (framboids) 1–30 μm in diameter within two weeks of the injection. The analyses confirmed that the remediating mixture and injection scheme reduced As concentrations to near or below the site’s clean-up standard of 0.05 mg/L over the nine months. Moreover, the arsenian pyrite contained 0.03–0.89 weight percentage (wt%) of sequestered arsenic, with >80% of groundwater arsenic removed by SRB biomineralization. Considering these promising findings, the study is close to optimizing an affordable procedure for sequestrating dissolved As in industry settings.
Investigation on Dynamical Mechanics, Energy Dissipation, and Microstructural Characteristics of Cemented Tailings Backfill under SHPB Tests
Authors: Zheng Di , Song Weidong , Cao Shuai , Li Jiajian , Sun Lijuan
Published: May 2021

As mining depth increases, the backfill mining method is more and more widely used in underground mines. The dynamic load generated by the blasting can affect the stability of the cemented tailings backfill (CTB). The CTB samples were prepared to conduct a test of the split Hopkinson pressure bar (SHPB) to investigate the dynamic disturbance of CTB. The present paper discusses dynamical mechanics, energy dissipation, and microstructure analysis of CTB. Micro-computer tomography (micro-CT) scanning of CTB samples after the SHPB test was performed to analyze the evolution of internal cracks. The experimental results showed that when the average strain rate (ASR) increased from 30 to 98 s−1, the dynamic uniaxial compression strength (DUCS) of the CTB showed a trend of first increasing and decreasing with the increase in ASR. The dynamic stress–strain pre-peak curve of CTB directly enters the linear elastic stage. As ASR increases, the absorbed energy of the CTB shows a trend of first increasing and then decreasing. Moreover, according to the micro-CT scanning results, the crack area of CTB accounts for about 16% of the sample near the incident bar and about 1% near the transmitted bar. The crack area ratio is exponentially related to the specimen height. These findings can provide reasonable dynamical CTB strength data selection for underground pillar mining.
Unusual Perforations in Phlogopite Crystals from Caldara di Manziana (Italy) Caused by Sulphuric Acid Generated by Microbial Oxidation of H2S Emanations
Authors: Pinzari Flavia , Cuadros Javier
Published: May 2021

Phlogopite flakes strewn on the soil of Caldara di Manziana (Italy) display multiple minute perforations. The site is a caldera linked to recent volcanism (90 ka to 0.8 Ma) with present emanations of CO2 (~150 t d−1) and H2S (~2.55 t d−1). Stereomicroscopy and SEM–EDX observation of the phlogopite crystals shows holes and depressions <200 µm to 2 mm across. They are circular, pseudo-hexagonal, or irregular. Within the depressions, there are deposits of phlogopite alteration products consistent with a sulphuric acid attack, showing loss of Mg and K. Some are thin and homogeneous; others are thick, irregular, and chemically heterogeneous, including plates, flakes, tubes of Fe-beidellite or Fe-bearing halloysite, silica, Fe oxides, and gypsum. Areas of phlogopite surface are also altered. Sulphuric acid is produced from the H2S gas by the mediation of sulphur-oxidizing bacteria. Pseudo-hexagonal perforations are interpreted to result from slow acid attack with dissolution controlled by phlogopite crystal symmetry. Some depressions developed surrounding films of pseudo-hexagonal shape, interpreted as jarosite crystallizing radially outwards from the depressions. This style of acid attack is possibly promoted by local high humidity and precipitation that generate long-lived water droplets and films on mineral surfaces where sulphuric acid is active for prolonged times.
Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley
Authors: Yao Haoyingye , Wang Yuxiao , Yin Junyi , Nie Shaoping , Xie Mingyong
Published: May 2021

Arabinoxylan (HBAX-60) was fractioned from alkaline-extracted arabinoxylan (HBAX) in the whole grain of hull-less barley (Hordeum vulgare L. var. nudum Hook. f. Poaceae) by 60% ethanol precipitation, which was studied for physicochemical properties and structure elucidation. Highly purified HBAX-60 mainly composed of arabinose (40.7%) and xylose (59.3%) was created. The methylation and NMR analysis of HBAX-60 indicated that a low-branched β-(1→4)-linked xylan backbone possessed un-substituted (1,4-linked β-Xylp, 36.2%), mono-substituted (β-1,3,4-linked Xylp, 5.9%), and di-substituted (1,2,3,4-linked β-Xylp, 12.1%) xylose units as the main chains, though other residues (α-Araf-(1→, β-Xylp-(1→, α-Araf-(1→3)-α-Araf-(1→ or β-Xylp-(1→3)-α-Araf-(1→) were also determined. Additionally, HBAX-60 exhibited random coil conformation in a 0.1 M NaNO3 solution. This work provides the properties and structural basis of the hull-less barley-derived arabinoxylan, which facilitates further research for exploring the structure–function relationship and application of arabinoxylan from hull-less barley.
Nanoformulation Development to Improve the Biopharmaceutical Properties of Fisetin Using Design of Experiment Approach
Authors: Liu Wan-Yi , Lin Chia-Chen , Hsieh Yun-Shan , Wu Yu-Tse
Published: May 2021

This study aimed to design an effective nanoparticle-based carrier for the oral delivery of fisetin (FST) with improved biopharmaceutical properties. FST-loaded nanoparticles were prepared with polyvinyl alcohol (PVA) and poly(lactic-co-glycolic acid) (PLGA) by the interfacial deposition method. A central composite design of two independent variables, the concentration of PVA and the amount of PLGA, was applied for the optimization of the preparative parameter. The responses, including average particle size, polydispersity index, encapsulation efficiency, and zeta potential, were assessed. The optimized formulation possessed a mean particle size of 187.9 nm, the polydispersity index of 0.121, encapsulation efficiency of 79.3%, and zeta potential of −29.2 mV. The morphological observation demonstrated a globular shape for particles. Differential scanning calorimetry and powder X-ray diffraction studies confirmed that the encapsulated FST was presented as the amorphous state. The dissolution test indicated a 3.06-fold increase for the accumulating concentrations, and the everted gut sac test showed a 4.9-fold gain for permeability at the duodenum region. In conclusion, the optimized FST-loaded nanoparticle formulation in this work can be developed as an efficient oral delivery system of FST to improve its biopharmaceutic properties.
Chemistry and Pharmacological Activity of Sesquiterpenoids from the Chrysanthemum Genus
Authors: Jiang Sai , Wang Mengyun , Jiang Zichen , Zafar Salman , Xie Qian , Yang Yupei , Liu Yang , Yuan Hanwen , Jian Yuqing , Wang Wei
Published: May 2021

Plants from the Chrysanthemum genus are rich sources of chemical diversity and, in recent years, have been the focus of research on natural products chemistry. Sesquiterpenoids are one of the major classes of chemical constituents reported from this genus. To date, more than 135 sesquiterpenoids have been isolated and identified from the whole genus. These include 26 germacrane-type, 26 eudesmane-type, 64 guaianolide-type, 4 bisabolane-type, and 15 other-type sesquiterpenoids. Pharmacological studies have proven the biological potential of sesquiterpenoids isolated from Chrysanthemum species, reporting anti-inflammatory, antibacterial, antitumor, insecticidal, and antiviral activities for these interesting molecules. In this paper, we provide information on the chemistry and bioactivity of sesquiterpenoids obtained from the Chrysanthemum genus which could be used as the scientific basis for their future development and utilization.
Design, Synthesis, and Antitumor Activity of Olmutinib Derivatives Containing Acrylamide Moiety
Authors: Hu Xiaohan , Tang Sheng , Yang Feiyi , Zheng Pengwu , Xu Shan , Pan Qingshan , Zhu Wufu
Published: May 2021

Two series of olmutinib derivatives containing an acrylamide moiety were designed and synthesized, and their IC50 values against cancer cell lines (A549, H1975, NCI-H460, LO2, and MCF-7) were evaluated. Most of the compounds exhibited moderate cytotoxic activity against the five cancer cell lines. The most promising compound, H10, showed not only excellent activity against EGFR kinase but also positive biological activity against PI3K kinase. The structure–activity relationship (SAR) suggested that the introduction of dimethylamine scaffolds with smaller spatial structures was more favorable for antitumor activity. Additionally, the substitution of different acrylamide side chains had different effects on the activity of compounds. Generally, compounds H7 and H10 were confirmed as promising antitumor agents.
Bioactive Components of Salvia and Their Potential Antidiabetic Properties: A Review
Authors: Abd Rashed Aswir , Rathi Devi-Nair Gunasegavan
Published: May 2021

The utilization of therapeutic plants is expanding around the globe, coupled with the tremendous expansion of alternative medicine and growing demand in health treatment. Plants are applied in pharmaceuticals to preserve and expand health—physically, mentally and as well as to treat particular health conditions and afflictions. There are more than 600 families of plants identified so far. Among the plants that are often studied for their health benefit include the genus of Salvia in the mint family, Lamiaceae. This review aims to determine the bioactive components of Salvia and their potential as antidiabetic agents. The search was conducted using three databases (PubMed, EMBASE and Scopus), and all relevant articles that are freely available in the English language were extracted within 10 years (2011–2021). Salvia spp. comprises many biologically active components that can be divided into monoterpenes, diterpenes, triterpenes, and phenolic components, but only a few of these have been studied in-depth for their health benefit claims. The most commonly studied bioactive component was salvianolic acids. Interestingly, S. miltiorrhiza is undoubtedly the most widely studied Salvia species in terms of its effectiveness as an antidiabetic agent. In conclusion, we hope that this review stimulates more studies on bioactive components from medicinal plants, not only on their potential as antidiabetic agents but also for other possible health benefits.
Ribes nigrum Leaf Extract Preferentially Inhibits IFN-γ-Mediated Inflammation in HaCaT Keratinocytes
Authors: Magnavacca Andrea , Piazza Stefano , Cammisa Anna , Fumagalli Marco , Martinelli Giulia , Giavarini Flavio , Sangiovanni Enrico , Dell’Agli Mario
Published: May 2021

Ribes nigrum L. (blackcurrant) leaf extracts, due to high levels of flavonols and anthocyanins, have been shown to exhibit beneficial effects in inflammatory diseases. However, whereas their traditional use has been investigated and validated in several models of inflammation and oxidative stress, the possible impact on skin disorders is still largely unknown. The purpose of this work was to elucidate the effects of R. nigrum leaf extract (RNLE) on keratinocyte-derived inflammatory mediators, elicited by a Th1 or Th2 cytokine milieu. HaCaT cells were challenged with TNF-α, either alone or in combination with the costimulatory cytokines IFN-γ or IL-4, and the release of proinflammatory cytokines and mediators (IL-8, IL-6, s-ICAM-1, and TSLP) was evaluated. The results showed that RNLE preferentially interferes with IFN-γ signaling, demonstrating only negligible activity on TNF-α or IL-4. This effect was attributed to flavonols, which might also account for the ability of RNLE to impair TNF-α/IL-4-induced TSLP release in a cAMP-independent manner. These results suggest that RNLE could have an antiallergic effect mediated in keratinocytes via mechanisms beyond histamine involvement. In conclusion, the discovery of RNLE preferential activity against IFN-γ-mediated inflammation suggests potential selectivity against Th1 type response and the possible use in Th1 inflammatory diseases.
Characterization of the Key Aroma-Active Compounds in Yongchuan Douchi (Fermented Soybean) by Application of the Sensomics Approach
Authors: Wang Shuqi , Chang Yuan , Liu Bing , Chen Haitao , Sun Baoguo , Zhang Ning
Published: May 2021

Yongchuan douchi is a traditional fermented soya bean product which is popular in Chinese dishes due to its unique flavor. In this study, the key aroma-active compounds of Yongchuan douchi were characterized by the combined gas chromatography–olfactometry (GC–O) and gas chromatography−mass spectrometry (GC–MS) with sensory evaluation. In total, 49 aroma compounds were sniffed and identified, and 20 of them with high flavor dilution factors (FD) and odor activity values (OAVs) greater than one were screened by applied aroma extract dilution analysis (AEDA) and quantitated analysis. Finally, aroma recombination and omission experiments were performed and 10 aroma-active compounds were thought to have contributed significantly including 2,3-butanedione (butter, cheese), dimethyl trisulfide (garlic-like), acetic acid (pungent sour), acetylpyrazine (popcorn-like), 3-methylvaleric acid (sweaty), 4-methylvaleric acid (sweaty), 2-mehoxyphenol (smoky), maltol (caramel), γ-nonanolactone (coconut-like), eugenol (woody) and phenylacetic acid (flora). In addition, sensory evaluation showed that the flavor profile of Yongchuan douchi mainly consisted of sauce-like, sour, nutty, smoky, caramel and fruity notes.
Identification of the Active Principle Conferring Anti-Inflammatory and Antinociceptive Properties in Bamboo Plant
Authors: Araujo Sousa Bruna , Nascimento Silva Osmar , Farias Porto William , Lima Rocha Thales , Paulino Silva Luciano , Ferreira Leal Ana Paula , Buccini Danieli Fernanda , Oluwagbamigbe Fajemiroye James , de Araujo Caldas Ruy , Franco Octávio Luiz , Grossi-de-Sá Maria Fátima , de la Fuente Nunez Cesar , Moreno Susana Elisa
Published: May 2021

Early plants began colonizing earth about 450 million years ago. During the process of coevolution, their metabolic cellular pathways produced a myriad of natural chemicals, many of which remain uncharacterized biologically. Popular preparations containing some of these molecules have been used medicinally for thousands of years. In Brazilian folk medicine, plant extracts from the bamboo plant Guadua paniculata Munro have been used for the treatment of infections and pain. However, the chemical basis of these therapeutic effects has not yet been identified. Here, we performed protein biochemistry and downstream pharmacological assays to determine the mechanisms underlying the anti-inflammatory and antinociceptive effects of an aqueous extract of the G. paniculata rhizome, which we termed AqGP. The anti-inflammatory and antinociceptive effects of AqGP were assessed in mice. We identified and purified a protein (AgGP), with an amino acid sequence similar to that of thaumatins (~20 kDa), capable of repressing inflammation through downregulation of neutrophil recruitment and of decreasing hyperalgesia in mice. In conclusion, we have identified the molecule and the molecular mechanism responsible for the anti-inflammatory and antinociceptive properties of a plant commonly used in Brazilian folk medicine.