bluuebunny/crossref_metadata_2025 huggingface dataset:
Immobilization of Fusarium solani Cutinase onto Magnetic Genipin-Crosslinked Chitosan Beads
Authors: Wang Zhanyong , Su Tingting , Zhao Jingjing
Published: September 2021

Genipin was used as a crosslinking agent to prepare magnetic genipin-crosslinked chitosan beads, which were then used as a carrier for immobilizing recombinant cutinase from Fusarium solani (FSC) to obtain immobilized FSC. The optimal temperature for the immobilized FSC was 55 °C, which was 5 °C higher than that of the free enzyme, whereas its optimal pH was increased from 8.0 to 9.0; this indicates that the immobilized FSC had improved pH and thermal stability. After repeated use for 10 cycles, the activity of the immobilized FSC remained at more than 50%; after being stored at 4 °C for 30 days, its activity was still approximately 88%. We also found that the Km of the immobilized FSC was higher than that of the free enzyme. These results indicate that the performance of FSC was improved after immobilization, which is an important basis for the subsequent application of FSC in industrial production.
CO2 Hydrogenation to Synthetic Natural Gas over Ni, Fe and Co–Based CeO2–Cr2O3
Authors: Khajonvittayakul Chalempol , Tongnan Vut , Amornraksa Suksun , Laosiripojana Navadol , Hartley Matthew , Hartley Unalome Wetwatana
Published: September 2021

CO2 methanation was studied over monometallic catalyst, i.e., Ni, Fe and Co; on CeO2-Cr2O3 support. The catalysts were prepared using one-pot hydrolysis of mixed metal nitrates and ammonium carbonate. Physicochemical properties of the pre- and post-exposure catalysts were characterized by X-Ray Powder Diffraction (XRD), Hydrogen Temperature Programmed Reduction (H2-TPR), and Field Emission Scanning Electron Microscope (FE-SEM). The screening of three dopants over CeO2-Cr2O3 for CO2 methanation was conducted in a milli-packed bed reactor. Ni-based catalyst was proven to be the most effective catalyst among all. Thus, a group of NiO/CeO2-Cr2O3 catalysts with Ni loading was investigated further. 40 % NiO/CeO2-Cr2O3 exhibited the highest CO2 conversion of 97.67% and CH4 selectivity of 100% at 290 °C. The catalytic stability of NiO/CeO2-Cr2O3 was tested towards the CO2 methanation reaction over 50 h of time-on-stream experiment, showing a good stability in term of catalytic activity.
Iridium Oxide Enabled Sensors Applications
Authors: Dong Qiuchen , Sun Xiangcheng , He Songbing
Published: September 2021

There have been numerous studies applying iridium oxides in different applications to explore their proton-change-based reactions since the 1980s. Iridium oxide can be fabricated directly by applying electrodeposition, sputter-coating method, or oxidation of iridium wire. Generally, there have been currently two approaches in applying iridium oxide to enable its sensing applications. One was to improve or create different electrolytes with (non-)electrodeposition method for better performance of Nernst Constant with the temperature-related system. The mechanism behind the scenes were summarized herein. The other was to change the structure of iridium oxide through different kinds of templates such as photolithography patterns, or template-assisted direct growth methods, etc. to improve the sensing performance. The detection targets varied widely from intracellular cell pH, glucose in an artificial sample or actual urine sample, and the hydrogen peroxide, glutamate or organophosphate pesticides, metal-ions, etc. This review paper has focused on the mechanism of electrodeposition of iridium oxide in aqueous conditions and the sensing applications towards different biomolecules compounds. Finally, we summarize future trends on Iridium oxide based sensing and predict future work that could be further explored.
Atomically Dispersed Catalytic Sites: A New Frontier for Cocatalyst/Photocatalyst Composites toward Sustainable Fuel and Chemical Production
Authors: Zhang Shuping , Bai Bing , Liu Jia , Zhang Jiatao
Published: September 2021

Photocatalysis delivers a promising pathway toward the clean and sustainable energy supply of the future. However, the inefficiency of photon absorption, rapid recombination of photogenerated electron-hole pairs, and especially the limited active sites for catalytic reactions result in unsatisfactory performances of the photocatalytic materials. Single-atom photocatalysts (SAPCs), in which metal atoms are individually isolated and stably anchored on support materials, allow for maximum atom utilization and possess distinct photocatalytic properties due to the unique geometric and electronic features of the unsaturated catalytic sites. Very recently, constructing SAPCs has emerged as a new avenue for promoting the efficiency of sustainable production of fuels and chemicals via photocatalysis. In this review, we summarize the recent development of SAPCs as a new frontier for cocatalyst/photocatalyst composites in photocatalytic water splitting. This begins with an introduction on the typical structures of SAPCs, followed by a detailed discussion on the synthetic strategies that are applicable to SAPCs. Thereafter, the promising applications of SAPCs to boost photocatalytic water splitting are outlined. Finally, the challenges and prospects for the future development of SAPCs are summarized.
PI3Kγ Mediates Microglial Proliferation and Cell Viability via ROS
Authors: Schmidt Caroline , Schneble-Löhnert Nadine , Lajqi Trim , Wetzker Reinhard , Müller Jörg P. , Bauer Reinhard
Published: September 2021

(1) Background: Rapid microglial proliferation contributes to the complex responses of the innate immune system in the brain to various neuroinflammatory stimuli. Here, we investigated the regulatory function of phosphoinositide 3-kinase γ (PI3Kγ) and reactive oxygen species (ROS) for rapid proliferation of murine microglia induced by LPS and ATP. (2) Methods: PI3Kγ knockout mice (PI3Kγ KO), mice expressing catalytically inactive PI3Kγ (PI3Kγ KD) and wild-type mice were assessed for microglial proliferation using an in vivo wound healing assay. Additionally, primary microglia derived from newborn wild-type, PI3Kγ KO and PI3Kγ KD mice were used to analyze PI3Kγ effects on proliferation and cell viability, senescence and cellular and mitochondrial ROS production; the consequences of ROS production for proliferation and cell viability after LPS or ATP stimulation were studied using genetic and pharmacologic approaches. (3) Results: Mice with a loss of lipid kinase activity showed impaired proliferation of microglia. The prerequisite of induced microglial proliferation and cell viability appeared to be PI3Kγ-mediated induction of ROS production. (4) Conclusions: The lipid kinase activity of PI3Kγ plays a crucial role for microglial proliferation and cell viability after acute inflammatory activation.
How Viruses Hijack and Modify the Secretory Transport Pathway
Authors: Hassan Zubaida , Kumar Nilima Dinesh , Reggiori Fulvio , Khan Gulfaraz
Published: September 2021

Eukaryotic cells contain dynamic membrane-bound organelles that are constantly remodeled in response to physiological and environmental cues. Key organelles are the endoplasmic reticulum, the Golgi apparatus and the plasma membrane, which are interconnected by vesicular traffic through the secretory transport route. Numerous viruses, especially enveloped viruses, use and modify compartments of the secretory pathway to promote their replication, assembly and cell egression by hijacking the host cell machinery. In some cases, the subversion mechanism has been uncovered. In this review, we summarize our current understanding of how the secretory pathway is subverted and exploited by viruses belonging to Picornaviridae, Coronaviridae, Flaviviridae, Poxviridae, Parvoviridae and Herpesviridae families.
Characterization of the Radiation Desiccation Response Regulon of the Radioresistant Bacterium Deinococcus radiodurans by Integrative Genomic Analyses
Authors: Eugénie Nicolas , Zivanovic Yvan , Lelandais Gaelle , Coste Geneviève , Bouthier de la Tour Claire , Bentchikou Esma , Servant Pascale , Confalonieri Fabrice
Published: September 2021

Numerous genes are overexpressed in the radioresistant bacterium Deinococcus radiodurans after exposure to radiation or prolonged desiccation. It was shown that the DdrO and IrrE proteins play a major role in regulating the expression of approximately twenty genes. The transcriptional repressor DdrO blocks the expression of these genes under normal growth conditions. After exposure to genotoxic agents, the IrrE metalloprotease cleaves DdrO and relieves gene repression. At present, many questions remain, such as the number of genes regulated by DdrO. Here, we present the first ChIP-seq analysis performed at the genome level in Deinococcus species coupled with RNA-seq, which was achieved in the presence or not of DdrO. We also resequenced our laboratory stock strain of D. radiodurans R1 ATCC 13939 to obtain an accurate reference for read alignments and gene expression quantifications. We highlighted genes that are directly under the control of this transcriptional repressor and showed that the DdrO regulon in D. radiodurans includes numerous other genes than those previously described, including DNA and RNA metabolism proteins. These results thus pave the way to better understand the radioresistance pathways encoded by this bacterium and to compare the stress-induced responses mediated by this pair of proteins in diverse bacteria.
NK Cell Patterns in Idiopathic Inflammatory Myopathies with Pulmonary Affection
Authors: Pawlitzki Marc , Nelke Christopher , Rolfes Leoni , Hasseli Rebecca , Tomaras Stylianos , Feist Eugen , Schänzer Anne , Räuber Saskia , Regner Liesa , Preuße Corinna , Allenbach Yves , Benveniste Olivier , Wiendl Heinz , Stenzel Werner , Meuth Sven G. , Ruck Tobias
Published: September 2021

Background: Pulmonary affection (PA) is associated with a substantial increase in morbidity and mortality in patients with idiopathic inflammatory myopathies (IIM). However, the underlying immune mechanisms of PA remain enigmatic and prompt deeper immunological analyses. Importantly, the Janus-faced role of natural killer (NK) cells, capable of pro-inflammatory as well as regulatory effects, might be of interest for the pathophysiologic understanding of PA in IIM. Methods: To extend our understanding of immunological alterations in IIM patients with PA, we compared the signatures of NK cells in peripheral blood using multi-color flow cytometry in IIM patients with (n = 12, of which anti-synthetase syndrome = 8 and dermatomyositis = 4) or without PA (n = 12). Results: We did not observe any significant differences for B cells, CD4, and CD8 T cells, while total NK cell numbers in IIM patients with PA were reduced compared to non-PA patients. NK cell alterations were driven by a particular decrease of CD56dim NK cells, while CD56bright NK cells remained unchanged. Comparisons of the cell surface expression of a large panel of NK receptors revealed an increased mean fluorescence intensity of NKG2D+ on NK cells from patients with PA compared with non-PA patients, especially on the CD56dim subset. NKG2D+ and NKp46+ cell surface levels were associated with reduced vital capacity, serving as a surrogate marker for clinical severity of PA. Conclusion: Our data illustrate that PA in IIM is associated with alterations of the NK cell repertoire, suggesting a relevant contribution of NK cells in certain IIMs, which might pave the way for NK cell-targeted therapeutic approaches.
Bruton’s Tyrosine Kinase Inhibitors: A New Generation of Promising Agents for Multiple Sclerosis Therapy
Authors: García-Merino Antonio
Published: September 2021

B cells play a central role in the pathogenesis of multiple sclerosis (MS), as demonstrated through the success of various B cell-depleting monoclonal antibodies. Bruton’s tyrosine kinase (BTK) is a critical molecule in intracellular signaling from the receptor of B cells and receptors expressed in the cells of the innate immune system. BTK inhibitors may be a non-cell-depleting alternative to B cell modulation. In this review, the structure, signaling, and roles of BTK are reviewed among the different inhibitors assayed in animal models of MS and clinical trials.
Comparison of Recurrence and Complication Rates Following Laparoscopic Inguinal Hernia Repair among Preterm versus Full-Term Newborns: A Systematic Review and Meta-Analysis
Authors: Pogorelić Zenon , Anand Sachit , Križanac Zvonimir , Singh Apoorv
Published: September 2021

Background: Laparoscopic inguinal hernia repair (LHR) in children has been widely performed in the last decades, although it is still not sufficiently researched in preterm infants. This systematic review and meta-analysis compared the recurrence and complication rates following laparoscopic hernia repair among preterm (PT) versus full-term (FT) newborns. Methods: Scientific databases (PubMed, EMBASE, Scopus, and Web of Science databases) were systematically searched for relevant articles. The following terms were used: (laparoscopic hernia repair) AND (preterm). The inclusion criteria were all preterm newborns with a unilateral or bilateral inguinal hernia who underwent LHR. The main outcomes were the incidence of recurrence of hernia and the proportion of children developing postoperative complications in comparison with FT newborns following LHR. Results: The present meta-analysis included four comparative studies. Three studies had a retrospective study design while one was a prospective study. A total of 1702 children were included (PT n = 523, FT n = 1179). The incidence of hernia recurrence showed no significant difference between the PT versus FT groups (RR = 2.58, 95% CI 0.89–7.47, p = 0.08). A significantly higher incidence of complications was observed in the PT group compared to the FT group (RR = 4.05, 95% CI 2.11–7.77, p < 0.0001). The PT group of newborns accounted for 81% and 72% of the major and minor complications. The major complications were either non-surgical (i.e., severe respiratory distress requiring reintubation with prolonged ventilation (or high-frequency ventilation), seizures, bradycardia), or surgical (i.e., hydroceles requiring operative intervention and umbilical port-site hernia). Conclusions: LHR in PT infants is associated with similar recurrence rates as in FT infants. However, the incidence of complications is significantly higher in PT versus FT infants.
Selective Attention and Concentration Are Related to Lifestyle in Chilean Schoolchildren
Authors: Caamaño-Navarrete Felipe , Latorre-Román Pedro Ángel , Párraga-Montilla Juan , Jerez-Mayorga Daniel , Delgado-Floody Pedro
Published: September 2021

The objective of this investigation was to determine the association between selective attention and concentration with physical fitness (i.e., cardiorespiratory fitness (CRF), V˙O2max, the standing long jump test (SLJ) and handgrip muscle strength (HGS)), lifestyle parameters (i.e., physical activity (PA) level, screen time (ST), sleep duration and food habits) and anthropometric measures (i.e., body mass index (BMI) and waist circumference (WC)) among Chilean schoolchildren. Two hundred and forty-eight schoolchildren (137 boys, 111 girls, 11.80 ± 1.17 and 11.58 ± 1.09 years, respectively) participated. Selective attention, concentration and lifestyle (PA, ST, sleep duration and Mediterranean diet (MD) adherence) were determined using a standard questionnaire. CRF, SLJ, HGS and anthropometric indicators (BMI and WC) were also measured. Selective attention showed a positive association with MD adherence score (β; 5.012, p = p < 0.05). Concentration was linked inversely to ST (β; −5.498, p = p < 0.05). Likewise, concentration presented a positive association with MD adherence (β; 2.904, p = p < 0.05). In conclusion, children’s lifestyles are related to the selective attention and concentration of children; therefore, promoting healthy habits could be a cost-effective strategy in the promotion of cognitive development, as it relates to selective attention and concentration.
Moisture Vapor Resistance of Coated and Laminated Breathable Fabrics Using Evaporative Wet Heat Transfer Method
Authors: Kim Hyun-Ah
Published: September 2021

This study examined the effects of the fiber materials, fabric structural parameters, and surface modification method on the moisture vapor resistance of coated and laminated fabrics according to the measuring method in comparison with evaporative wet heat transfer method. The moisture vapor resistance (Ref) of the coated and laminated fabrics measured using evaporative wet heat transfer method was much more precise than water vapor transmission rate (WVTR) and water vapor permeability (WVP) measured using American Society for Testing and Materials (ASTM) and Japanese Industrial Standard (JIS) methods. The correlation coefficient between Ref and WVTR in the laminated and coated polyethylene terephthalate (PET) fabric specimens was the highest, i.e., −0.833, and −0.715, in coated fabric specimens. Hence, selecting an appropriate measuring method according to the fabric materials and surface modification method is very critical. According to curvilinear regression analysis, the influential factor affecting breathability of the PET fabric specimens measured using evaporative wet heat transfer method was fabric weight (R2 = 0.847) and fabric thickness (R2 = 0.872) in the laminated fabric specimens. Meanwhile, as per multiple linear regression, the most influential fabric structural parameters affecting the breathability of laminated fabric specimens measured using evaporative wet heat transfer method were the fabric density, weight/thickness, and weight followed by the fabric thickness (R2 = 0.943). These results would be valid for laminated breathable fabrics with characteristics within the range of this study and are of practical use for engineering laminated fabrics with high breathability.
Interactions in Composite Film Formation of Mefp-1/graphene on Carbon Steel
Authors: Cheng Jie , Mei Nanxuan , Chen Sulin , Bai Pengpeng , Shen Bin , Pan Jinshan , Zhang Fan
Published: September 2021

Mefp-1 adhesive protein derived from marine blue mussels, together with the 2D material graphene, was used to build the green composite film with enhanced anti-corrosion property and mechanical strength. The corrosion inhibition of the composite film, formed by different methods, was evaluated by using electrochemical impedance spectroscopy. The non-degraded adhesion of the composite film to the carbon steel substrate was proved by nano-scratch tests. Infrared spectroscopy was utilized to investigate the film formation process and “three-body interactions” between Mefp-1, graphene and carbon steel surface. The results show that the Mefp-1 adsorbs on the carbon steel surface mainly through the covalent bond between catechols and Fe(III). Meanwhile, Mefp-1 can bond to non-adhesive graphene by forming hydrogen bonds and π−π interaction non-covalent bonds, which facilitate the formation of a robust Mefp-1/graphene composite film on the carbon steel surface.
Superhydrophobic Al2O3–Polymer Composite Coating for Self-Cleaning Applications
Authors: Sutar Rajaram S. , Nagappan Saravanan , Bhosale Appasaheb K. , Sadasivuni Kishor Kumar , Park Kang-Hyun , Ha Chang-Sik , Latthe Sanjay S.
Published: September 2021

Superhydrophobic coatings have a huge impact in various applications due to their extreme water-repellent properties. The main novelty of the current research work lies in the development of cheap, stable, superhydrophobic and self-cleaning coatings with extreme water-repellency. In this work, a composite of hydrothermally synthesized alumina (Al2O3), polymethylhydrosiloxane (PMHS) and polystyrene (PS) was deposited on a glass surface by a dip-coating technique. The Al2O3 nanoparticles form a rough structure, and low-surface-energy PHMS enhances the water-repellent properties. The composite coating revealed a water contact angle (WCA) of 171 ± 2° and a sliding angle (SA) of 3°. In the chemical analysis, Al2p, Si2p, O1s, and C1s elements were detected in the XPS survey. The prepared coating showed a self-cleaning property through the rolling action of water drops. Such a type of coating could have various industrial applications in the future.
Influence of the Sputtering Technique and Thermal Annealing on YSZ Thin Films for Oxygen Sensing Applications
Authors: Paz Alpuche Emilio , Gröger Pascal , Wang Xuetao , Kroyer Thomas , Fasoulas Stefanos
Published: September 2021

Yttria-stabilized zirconia (YSZ) thin films were deposited using direct current (reactive and metallic) and radio frequency magnetron sputtering. The effect of the deposition technique and annealing treatment on the microstructure and crystallinity of the thin films was assessed. Using the films produced in this work, oxygen gas sensors were built and their performance under vacuum conditions was evaluated. All the films exhibited a cubic crystalline structure after a post-deposition thermal treatment, regardless of the sputtering technique. When the annealing treatment surpassed 1000 °C, impurities were detected on the thin film surface. The oxygen gas sensors employing the reactive and oxide-sputtered YSZ thin films displayed a proportional increase in the sensor current as the oxygen partial pressure was increased in the evaluated pressure range (5 × 10−6 to 2 × 10−3 mbar). The sensors which employed the metallic-deposited YSZ films suffered from electronic conductivity at low partial pressures.
The Third Global Coral Bleaching Event on the Marginal Coral Reefs of the Southwestern Indian Ocean and Factors That Contribute to Their Resistance and Resilience
Authors: Porter Sean N. , Sink Kerry J. , Schleyer Michael H.
Published: September 2021

Coral reefs reach their southernmost limits in the southwestern Indian Ocean in Maputaland, South Africa. Here, we investigate the recent global coral bleaching event of 2016, the thermal dynamics of these marginal high-latitude reefs and the potential environmental factors regulating the responses of coral communities. Pre-, peak- and post-bleaching surveys of over 9850 coral colonies from 29 genera were undertaken over 3 years across 14 sites spanning 120 km of coastline using point-intercept and visual bleaching index survey methodologies. Bleaching data were related to several environmental variables including temperature, degree heating weeks (DHW), depth, latitude, and upwelling intensity. These reefs have experienced a history of relatively low thermal stress based on DHW. Long-term in situ temperature records nevertheless showed no obvious trend of increase. In situ temperatures also displayed poor relationships, with temperatures predicted by the Representative Concentration Pathway models. Mild coral bleaching with no significant mortality was recorded across sites with taxon-specific bleaching responses evident. Latitude and cumulative daily DHW were significantly related to the bleaching index whereas depth and interactions of depth with latitude and DHW were not. While upwelling of cooler water may offer some refuge to coral communities, especially in the Central and Southern Reef Complexes where it is more pronounced, this may only be transient as the upwelled water may also experience some degree of warming in future, thereby limiting such protection from global warming.
Performance of a Surrogate SARS-CoV-2-Neutralizing Antibody Assay in Natural Infection and Vaccination Samples
Authors: Chan Kwok-Hung , Leung Ka-Yi , Zhang Ricky-Ruiqi , Liu Danlei , Fan Yujing , Chen Honglin , Yuen Kwok-Yung , Hung Ivan Fan-Ngai
Published: September 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibody (NAb) production is a crucial humoral response that can reduce re-infection or breakthrough infection. The conventional test used to measure NAb production capacity levels is the live virus-neutralizing assay. However, this test must be conducted under biosafety level-3 containment. Pseudovirus or surrogate NAb tests, such as angiotensin-converting enzyme 2 inhibition tests, can be performed under level-2 containment. The aim of this study was to evaluate the performance of a surrogate SARS-CoV-2 NAb assay (sNAb) using samples from naturally infected individuals and vaccine recipients in comparison with the live virus microneutralization assay (vMN). Three hundred and eighty serum samples which were collected from 197 patients with COVID-19, 96 vaccine recipients and 84 normal individuals were analyzed. Overall, the sensitivity, specificity, positive predictive value, and negative predictive value of the sNAb (iFlash-2019-NAb assay, Shenzhen, China) were 97.9%, 94.9%, 98.2%, and 93.8%, respectively. Agreement for the assay relative to vMN for naturally infected individuals and vaccine recipients were 98.5% and 93.9%, respectively. A correlation analysis between sNAb and the vMN for both of these groups yielded an R2 value of 0.83. The iFlash RBD NAb assay is found to be sensitive and reliable for neutralizing antibody measurement in patients with the 2019 coronavirus disease and those who have been vaccinated against it.
Whole-Body MRI in Rheumatology: Major Advances and Future Perspectives
Authors: Deplano Luca , Piga Matteo , Porcu Michele , Stecco Alessandro , Suri Jasjit S. , Mannelli Lorenzo , Cauli Alberto , Carriero Alessandro , Saba Luca
Published: September 2021

Whole-body magnetic resonance imaging is constantly gaining more importance in rheumatology, particularly for what concerns the diagnosis, follow-up, and treatment response evaluation. Initially applied principally for the study of ankylosing spondylitis, in the last years, its use has been extended to several other rheumatic diseases. Particularly in the pediatric population, WB-MRI is rapidly becoming the gold-standard technique for the diagnosis and follow-up of both chronic recurrent multifocal osteomyelitis and juvenile spondyloarthritis. In this review, we analyze the benefits and limits of this technique as well as possible future applications.
Medullary Carcinoma of the Gastrointestinal Tract: Report on Two Cases with Immunohistochemical and Molecular Features
Authors: Colarossi Cristina , Mare Marzia , La Greca Giorgio , De Zuanni Marco , Colarossi Lorenzo , Aiello Eleonora , Piombino Eliana , Memeo Lorenzo
Published: September 2021

Medullary carcinoma of the colon is a rare histological variant characterized by a poorly differentiated morphology, an aberrant immunophenotype, and microsatellite instability. Despite the lack of glandular differentiation, medullary carcinoma is reported to have a good prognosis. It is typically located in the right colon and frequently affects older women. Due to its clinical, histological, biological, and genetic peculiarity, medullary carcinoma requires an accurate diagnosis and the awareness of this diagnostic possibility. We describe the morphological, immunohistochemical, and molecular findings of two interesting cases, the first one in the right colon of a patient and the second one in the terminal ileum of a patient with Crohn’s disease. Deeper knowledge of all the biological and clinical features will allow appropriate and specific treatment of this tumor in the future.
Assessment of Tooth Preparations Submitted to Dental Laboratories for Fabrication of Monolithic Zirconia Crowns
Authors: Sadid-Zadeh Ramtin , Sahraoui Hadjer , Lawson Brian , Cox Robert
Published: September 2021

Purpose: The objective of this study was to assess the quality of posterior teeth prepared for monolithic zirconia crowns. Materials and Methods: A total of 392 STL-files of posterior preparations for monolithic zirconia crowns were evaluated in this study. Three-dimensional (3D) images were evaluated using a software (3D Viewer; 3Shape A/S, Copenhagen, Denmark) for finish line design, finish line width, occluso-cervical dimension, total occlusal convergence (TOC), intercuspal angulation, finish line quality, line angle form, and presence or absence of undercut at the axial wall and unsupported lip of enamel. The assessment was performed by two calibrated evaluators. Then, data were descriptively analyzed. Data for occluso-cervical dimension and TOC were descriptively analyzed according to their location. Results: Thirty-nine percent of premolars, 77% of first molars, and 91% of second molars had an average occluso-cervical dimension of less than 3 mm (premolars) and 4 mm (molars), with most of the preparations having a TOC of more than 20 degrees. More than 50% of preparations had undercut, unsupported enamel and/or unacceptable finish line quality. Conclusions: The quality of tooth preparation including finish line quality, absence of unsupported enamel and undercut at the axial wall should be evaluated when preparing monolithic zirconia crowns.
Intelligent Reflective Surfaces for Wireless Networks: An Overview of Applications, Approached Issues, and Open Problems
Authors: Pérez-Adán Darian , Fresnedo Óscar , González-Coma José P. , Castedo Luis
Published: September 2021

An intelligent reflective surface (IRS) is a novel and revolutionizing communication technology destined to enable the control of the radio environment. An IRS is a real-time controllable reflectarray with a massive number of low-cost passive elements which introduce a phase shift to the incoming signals from the sources before the propagation towards the destination. This technology introduces the notion of a smart propagation environment with the aim of improving the system performance. In this paper, we provide a comprehensive literature overview on IRS technology, including its basic concepts and reconfiguration, as well as its design aspects and applications for wireless communication systems. We also study the performance metrics and the setups considered in recent publications related to IRS and provide suggestions of future research lines based on still unexplored use cases in the state-of-the-art.
Cricket Match Analytics Using the Big Data Approach
Authors: Awan Mazhar Javed , Gilani Syed Arbaz Haider , Ramzan Hamza , Nobanee Haitham , Yasin Awais , Zain Azlan Mohd , Javed Rabia
Published: September 2021

Cricket is one of the most liked, played, encouraged, and exciting sports in today’s time that requires a proper advancement with machine learning and artificial intelligence (AI) to attain more accuracy. With the increasing number of matches with time, the data related to cricket matches and the individual player are increasing rapidly. Moreover, the need of using big data analytics and the opportunities of utilizing this big data effectively in many beneficial ways are also increasing, such as the selection process of players in the team, predicting the winner of the match, and many more future predictions using some machine learning models or big data techniques. We applied the machine learning linear regression model to predict the team scores without big data and the big data framework Spark ML. The experimental results are measured through accuracy, the root mean square error (RMSE), mean square error (MSE), and mean absolute error (MAE), respectively 95%, 30.2, 1350.34, and 28.2 after applying linear regression in Spark ML. Furthermore, our approach can be applied to other sports.
Body-Effect-Free OLED-on-Silicon Pixel Circuit Based on Capacitive Division to Extend Data Voltage Range
Authors: Bae Jina , Nam Hyoungsik
Published: September 2021

This paper proposes an OLED pixel compensation circuit that copes with threshold voltage variation, narrow data voltage range, and body effect on a backplane of silicon-based transistors. It consists of six PMOS transistors and two capacitors. The data voltage range is extended by the capacitor division with two capacitors, and the connection of both source and gate nodes to the supply voltage makes the driving transistor free from the body effect. In addition, the reference voltage is used to initialize the gate node voltage of the driving transistor as well as to adjust the data voltage region. By the SPICE simulation, it is verified that the current error over the threshold voltage variations of ±10 mV is reduced to be −1.200% to 0.964% at the maximum current range of around 8 nA, and the data voltage range is extended to 3.4 V, compared to the large current error range from −21.46% to 27.36% and the data voltage range of 0.41 V in the basic 2T1C circuit. In addition, the body-effect-free circuit outperforms the latest 4T1C circuit of the current error range from −3.279% to 3.388%.
Green Building Rating Systems (GBRSs)
Authors: Marchi Lia , Antonini Ernesto , Politi Stefano
Published: September 2021

Green Building Rating Systems (GBRSs) are typically third-party, voluntary, and market driven standards that measure buildings’ sustainability level by multi-criteria assessment, and encourage the adoption of environmentally, socially and economically sustainable practices in design, construction and operation of buildings (or neighborhoods). GBRSs aim at guiding and assessing the project throughout all its life cycle, thus limiting the negative impact on the environment, as well as on the building occupants’ health and well-being, and even reducing operational costs. Hundreds of GBRSs are now available worldwide, varying in approaches, application processes, and evaluation metrics. BREEAM, CASBEE, Green Star and LEED are among the most applied worldwide. Despite some differences, they all adhere to the same general evaluation structure: project performances ares measured using a set of relevant indicators, grouped per topics such as water management, energy use, materials, site qualities. Each assessed requirement is assigned a score/judgment, the total of which determines the level of sustainability achieved. In addition to regular updates, a current trend is to improve the effectiveness of protocols, making them more comprehensive and accurate, while keeping them easy to use.
Morpho-Physiological and Biochemical Changes in Syzygium cumini and Populus deltoides: A Case Study on Young Saplings under Water Stress
Authors: Zafar Zikria , Rasheed Fahad , Khan Waseem Razzaq , Afzal Shazia , Qadeer Abdual , Mohsin Muhammad , Nazre Mohd , Raza Zohaib , Ibrahim Faridah Hanum
Published: September 2021

Drought is one of the most devastating climate factors in terms of its spatial extent and intensity. Therefore, a study was conducted to evaluate the water stress tolerance in young saplings of Syzygium cumini (L.) Skeels and Populus deltoides Marchall that are cultivated in the rain fed areas of Pakistan. Plants were subjected to three levels of moisture regimes: well-watered (WW, 90% of field capacity), mild stress (MS, 60% field capacity), and severe stress (SS, 30% of field capacity). Results showed that dry biomass production (leaf, stem, and root), chlorophyll a, b and carotenoid contents decreased significantly while osmolyte accumulation increased in both species, with the highest increase was evidenced in Populus deltoides saplings. A significant decrease was evidenced in CO2 assimilation rate and stomatal conductance that resulted in a significant increase in intrinsic water use efficiency in both species under MS and SS. In both the species, along with a significant increase in the production of hydrogen peroxide and superoxide radical, the antioxidants enzyme activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase also increased significantly in both species under MS and SS with highest activity evidenced in Syzygium cumini. The results suggest that Syzygium cumini saplings showed better a tolerance mechanism to water stress.
An Indicator-Based Approach to Assess the Readiness of Urban Forests for Future Challenges: Case Study of a Mediterranean Compact City
Authors: Maradiaga-Marín Mª Fernanda , Cariñanos Paloma
Published: September 2021

Urban Forests (UFs) are key elements in Mediterranean compact cities, as they provide numerous ecosystem benefits and increase the resilience of cities against the anticipated impacts of climate change. It is, thus, necessary to review all the aspects that may have a negative effect on their ecosystem functions and the services that they provide. In this paper, a set of indicators is proposed that allow for a preliminary evaluation of some of the main disservices and factors that Mediterranean UFs present and the ways to maximize their benefits for users. For this purpose, 20 indicators, divided into three categories—Biodiversity, Accessibility/Facilities, and Infrastructure—were selected. Within these three categories, a range of values was established, from low to high or absence/presence. The indicators were tested in 24 urban forests of different types, all of which are representative of a medium-sized compact Mediterranean city. The results highlight that the UFs have adequate species richness and diversity, but among the species present are quite a few that emit BVOCs and allergens, as well as some that have invasive behavior. Poor cleaning, absence of night lightning, and scarcity of water points are aspects to improve in a good number of UFs; while a high surface area of impermeable soil, low tree cover, and extensive areas of grass that require large amounts of water for maintenance are the main issues of the infrastructure block that need medium-term planning to be addressed. It can be concluded that the proposed set of indicators allows for a general assessment of the readiness of UFTs in Mediterranean cities for the upcoming climatic, social, and ecological challenges.
Soil Fungal Communities and Enzyme Activities along Local Tree Species Diversity Gradient in Subtropical Evergreen Forest
Authors: Fu Ziqi , Chen Qin , Lei Pifeng , Xiang Wenhua , Ouyang Shuai , Chen Liang
Published: September 2021

The majority of studies have found that an increase in tree species diversity can increase the productivity of forest stands thanks to complimentary effects with enhanced resource use efficiency or selection effects; however, it is unclear how tree species diversity affects the soil fungal community and enzyme activities in subtropical evergreen forests. In this study, we used soil high-throughput sequencing to investigate the soil fungal community structure and diversity in the central area of tree clusters in the gradient of tree species richness formed by four possible dominant tree species (Pinus massoniana Lamb., Choerospondias axillaris Roxb., Cyclobalanopsis glauca Thunb. and Lithocarpus glaber Thunb.) in subtropical evergreen broad-leaved forest. The results showed that soil organic carbon content and total nitrogen content were significantly higher in mixed tree clusters, and that soil fungal richness and diversity increased with the increase in tree species diversity (1–3 species). Soil acid phosphatase and urease activity were also enhanced with tree species diversity (p < 0.05). The relative abundance of soil symbiotic fungi (ectomycorrhizal fungi) decreased, while the relative abundance of saprotrophic fungi increased. Redundancy analysis (RDA) revealed that soil acid phosphatase activity was the main factor affecting soil fungal communities and functional guilds, and that soil water content was the main driving force behind fungal trophic modes. In subtropical forests, changes in tree species diversity have altered the soil fungal community structure and trophic modes and functions, accelerating the decomposition of organic matter, increasing nutrient cycling, and perhaps also changing the nutrient absorption of trees.
Trefoil Factor Family Member 2 Expression as an Indicator of the Severity of the High-Fat Diet-Induced Obesity
Authors: Ghanemi Abdelaziz , Yoshioka Mayumi , St-Amand Jonny
Published: September 2021

Trefoil Factor Family Member 2 (TFF2) belongs to TFF family peptides that includes TFF1, TFF2, TFF3. TFF2 is mainly known for its roles in the mucosal protection. In the context of obesity and high fat diet (HFD), Tff2 has been characterized as a HFD-induced gene. The knock-out of Tff2 in mice lead to the protection from HFD-induced obesity with a metabolic profile towards a negative energy balance. Such HFD-specific expression gives Tff2 a pattern worth exploring in biomedical research. Indeed, measuring TFF2/TFF2/Tff2 expression in biological samples following the ingestion of high-fat diet reflects the biological “responsiveness” to the lipids ingestion and would reflect the severity of obesity establishment afterwards. Such property could be explored for instance to screen animal models, evaluate the predisposition to HFD-induced obesity as well as in biomedical and clinical applications. Results might advance obesity research especially in terms of understanding lipid-induced signals, appetite control and adiposity storage.
Drosophila Models for Charcot–Marie–Tooth Neuropathy Related to Aminoacyl-tRNA Synthetases
Authors: Morant Laura , Erfurth Maria-Luise , Jordanova Albena
Published: September 2021

Aminoacyl-tRNA synthetases (aaRS) represent the largest cluster of proteins implicated in Charcot–Marie–Tooth neuropathy (CMT), the most common neuromuscular disorder. Dominant mutations in six aaRS cause different axonal CMT subtypes with common clinical characteristics, including progressive distal muscle weakness and wasting, impaired sensory modalities, gait problems and skeletal deformities. These clinical manifestations are caused by “dying back” axonal degeneration of the longest peripheral sensory and motor neurons. Surprisingly, loss of aminoacylation activity is not a prerequisite for CMT to occur, suggesting a gain-of-function disease mechanism. Here, we present the Drosophila melanogaster disease models that have been developed to understand the molecular pathway(s) underlying GARS1- and YARS1-associated CMT etiology. Expression of dominant CMT mutations in these aaRSs induced comparable neurodegenerative phenotypes, both in larvae and adult animals. Interestingly, recent data suggests that shared molecular pathways, such as dysregulation of global protein synthesis, might play a role in disease pathology. In addition, it has been demonstrated that the important function of nuclear YARS1 in transcriptional regulation and the binding properties of mutant GARS1 are also conserved and can be studied in D. melanogaster in the context of CMT. Taken together, the fly has emerged as a faithful companion model for cellular and molecular studies of aaRS-CMT that also enables in vivo investigation of candidate CMT drugs.
Instability of Compacted Residual Soil
Authors: Junaideen Sainulabdeen Mohamed , Tham Leslie George , Lee Chack Fan
Published: September 2021

Static liquefaction of loose sands has been observed to initiate at stress ratios far less than the steady-state stress ratio. Different collapse surface concepts largely based on undrained triaxial test results have been proposed in the literature to explain the above instability phenomenon of loose sands. Studies of the instability behavior of fill material derived from residual soils remain limited. The present study investigated the instability behavior of a compacted residual soil using the conventional undrained triaxial tests and specially equipped constant shear triaxial tests. The test results were characterized in the p’: q: v space using the current state parameter with respect to the steady-state line for the residual soil. A modified collapse surface that has gradients varying with p’ and v was proposed for the loose residual soil to represent the instability states of undrained loading. Under constant shear stress conditions, the soil can mobilize stress ratios higher than those defined by the modified collapse surface. An instability surface was therefore presented for the instability states reached in static loading. Further, an alternative method of deducing the instability surface from the undrained stress paths was introduced.
Reconstructing the Gorte and Spiaz de Navesele Landslides, NE of Lake Garda, Trentino Dolomites (Italy)
Authors: Ruggia Giacomo , Ivy-Ochs Susan , Aaron Jordan , Steinemann Olivia , Martin Silvana , Rigo Manuel , Rossato Sandro , Vockenhuber Christof , Monegato Giovanni , Viganò Alfio
Published: September 2021

We applied a multi-method approach to reconstruct the Gorte rock avalanche (85–95 Mm3) located at the northeastern end of Lake Garda. The combination of field mapping, characterization of bedrock discontinuities, Dan3D-Flex runout modeling and dating of boulders with cosmogenic 36Cl supports the conclusion that the deposits stem from a single rock avalanche at 6.1 ± 0.8 ka. The Gorte event may have triggered the Spiaz de Navesele–Salto della Capra landslide (3.2 Mm3), whose deposits cover the southern end of the Gorte deposits. First-order controls on detachment were the NNE–SSW- and WNW–ESE-oriented fractures in the limestone bedrock, related to the Giudicarie and Schio-Vicenza fault systems, respectively. Dan3D-Flex runout modeling sufficiently reproduced the Gorte rock avalanche, which involved detachment and sliding of a quasi-intact block, likely along marly interbeds, followed by rapid disintegration. The frictional rheology in the source area and the turbulent frictional rheology (Voellmy) in the remaining part best replicate the observed deposit extent and thickness. Heavy precipitation that occurred at that time may have contributed to failure at Gorte. Nonetheless, its timing overlaps with the nearby (<15 km) Dosso Gardene (6630–6290 cal BP) and Marocca Principale (5.3 ± 0.9 ka) landslides, making a seismic trigger plausible.
Pilot Study of Eco-Physiological Pepper Responses to Starfish-Based Organic Soil Amendments in Open-Field and Greenhouse Cultivations
Authors: Choi Hyun-Sug
Published: September 2021

This pilot study was conducted to compare eco-physiological responses to starfish (SF)-treated red peppers (Capsicum annuum L.) in organic open-field (OF) and greenhouse (GH) cultivations in commercial farmhouses, South Korea in 2019. Treatments included starfish-liquid fertilizer (LF) applied in OF (SF-OF) and GH (SF-GH) plots, and SF + seaweed in OF (SFS-OF) and GH (SFS-GH). Weekly pH levels in SFS-LF were fluctuated for a 16-week storage period at room temperature due to having higher soluble salt levels than those of SF-LF. All experimental plots were ranged on soil pH between 7.1 and 7.4. SF- and SFS-GH plots resulted in increased soil electrical conductivity, organic matter, and increased concentrations of total nitrogen, phosphorous pentoxide, potassium oxide, and magnesium oxide, as well as exhibiting a richer and more diverse bacterial community. Leaf width and length and plant height increased in plants cultivated in GH, with low canopy width and stem diameter also observed to have increased. Total fruit yields were approximately two times higher for peppers cultivated in GH compared to OF cultivated peppers.
The Impact of the COVID-19 Pandemic on the Working Lives and Retirement Timing of Older Nurses in Ireland
Authors: Ní Léime Áine , O’Neill Margaret
Published: September 2021

COVID-19 profoundly affected Irish citizens. The effects have been especially pronounced for nurses in front-line, clinical and management roles. This article discusses the national and employer policy context relevant to nurses in Ireland. There have been staff and bed shortages in public hospitals since austerity policies were introduced following the global financial crisis. Government measures responding to the pandemic include initial ‘cocooning’ of older citizens, travel restrictions, changed working conditions and restricted availability of childcare. This article draws on interviews with 25 older nurses in 2021, sixteen women and nine men, aged 49 or over in Ireland. It explores older nurses’ experiences of COVID-19 and asks what are the implications for their working conditions and retirement timing intentions. A gendered political economy of ageing approach and thematic analysis reveals that while some nurses responded positively to the pandemic, some experienced adverse health impacts, stress and exhaustion; some reported a fear of contracting COVID-19 and of infecting their families; several women nurses decided to retire earlier due to COVID-19. The implications of the findings for employer and government policy and for research are discussed.