bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification and Characterization of Gibberellic Acid-Stimulated Arabidopsis Gene Family in Pineapple (Ananas comosus)
Authors: Yang Mingzhe , Liu Chaoyang , Zhang Wei , Wu Jing , Zhong Ziqin , Yi Wen , Liu Hui , Leng Yan , Sun Weisheng , Luan Aiping , He Yehua
Published: December 2023

The gibberellic acid-stimulated Arabidopsis (GASA) gene family plays a crucial role in growth, development, and stress response, and it is specific to plants. This gene family has been extensively studied in various plant species, and its functional role in pineapple has yet to be characterized. In this study, 15 AcGASA genes were identified in pineapple through a genome-wide scan and categorized into three major branches based on a phylogenetic tree. All AcGASA proteins share a common structural domain with 12 cysteine residues, but they exhibit slight variations in their physicochemical properties and motif composition. Predictions regarding subcellular localization suggest that AcGASA proteins are present in the cell membrane, Golgi apparatus, nucleus, and cell wall. An analysis of gene synteny indicated that both tandem and segmental repeats have a significant impact on the expansion of the AcGASA gene family. Our findings demonstrate the differing regulatory effects of these hormones (GA, NAA, IAA, MeJA, and ABA) on the AcGASA genes. We analyzed the expression profiles of GASA genes in different pineapple tissue parts, and the results indicated that AcGASA genes exhibit diverse expression patterns during the development of different plant tissues, particularly in the regulation of floral organ development. This study provides a comprehensive understanding of GASA family genes in pineapple. It serves as a valuable reference for future studies on the functional characterization of GASA genes in other perennial herbaceous plants.
A Transcriptome Array-Based Approach to Link SGLT-2 and Intrarenal Complement C5 Synthesis in Diabetic Nephropathy
Authors: Korsten Peter , Tampe Björn
Published: December 2023

Diabetic nephropathy is a common microvascular complication of diabetes mellitus. It is characterized by progressive chronic kidney disease (CKD) with decline of kidney function by hyperfiltration. On a mechanistic level, activation of the complement system has been implicated in the pathogenesis of diabetic nephropathy. Therefore, here we pursued a transcriptome array-based approach to link intrarenal SGLT-2 and the synthesis of distinct complement components in diabetic nephropathy. Publicly available datasets for SLC5A2 (encoding SGLT-2) and complement system components were extracted specifically from microdissected tubulointerstitial (healthy controls: n = 31, diabetic nephropathy: n = 17) and glomerular compartments (healthy controls: n = 21, diabetic nephropathy: n = 12). First, we compared tubulointerstitial and glomerular log2 SLC5A2 mRNA expression levels and confirmed a predominant synthesis within the tubulointerstitial compartment. Among various complement components and receptors, the only significant finding was a positive association between SLC5A2 and the tubulointerstitial synthesis of the complement component C5 in diabetic nephropathy (p = 0.0109). Finally, intrarenal expression of SLC5A2 was associated predominantly with pathways involved in metabolic processes. Interestingly, intrarenal complement C5 synthesis was also associated with enrichment of metabolic signaling pathways, overlapping with SLC5A2 for “metabolism” and “biological oxidations”. These observations could be of relevance in the pathogenesis of diabetic nephropathy and implicate a mechanistic link between SGLT-2 and intrarenal complement synthesis.
Association between Nfr2, HO-1, NF-kB Expression, Plasma ADMA, and Oxidative Stress in Metabolic Syndrome
Authors: Bekyarova Ganka Y. , Vankova Deyana G. , Madjova Valentina H. , Bekyarov Nicolai A. , Salim Ayshe S. , Ivanova Diana G. , Stoeva Stefka M. , Gerova Daniela I. , Kiselova-Kaneva Yoana D.
Published: December 2023

Endothelial dysfunction is one of the major factors in the pathogenesis of metabolic syndrome (MetS), and its molecular mechanisms are not completely understood. The present study aimed to examine the connection between nuclear factor2-related factor2 (Nrf2), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), heme oxygenase 1 (HO-1), and plasma asymmetric dimethylarginine (ADMA) and malondialdehyde (MDA) in people with MetS. Participants in the study were as follows: with MetS (n = 30) and without MetS (Control) (n = 14). Expression of Nrf2, NF-kB, and HO-1 was measured in peripheral blood mononuclear cells (PBMCs). Plasma ADMA was determined using the ELISA technique and MDA via the thiobarbituric acid method. Our study showed that mRNA of NF-kB, Nrf2, and HO-1 levels in PBMCs in the MetS group were significantly higher than in the controls by 53%, 130%, and 185% (p < 0.05), respectively. Similarly, elevated levels of MDA (by 78%, p < 0.001) and ADMA (by 18.7%, p < 0.001) were established in the MetS group. Our findings show the importance of transcription factor Nrf2, playing an integral role in the protection of the endothelium, and of NF-κB, a transcription factor mediating the inflammatory response in MetS. Knowledge of complex cellular–molecular mechanisms would allow the use of biomarkers such as Nrf2, NF-kB, HO-1, and ADMA for the assessment of endothelial dysfunction in clinical practice.
Mitochondrially Targeted Gene Therapy Rescues Visual Loss in a Mouse Model of Leber’s Hereditary Optic Neuropathy
Authors: Chou Tsung-Han , Hao Zixuan , Alba Diego , Lazo Angelina , Gallo Afflitto Gabriele , Eastwood Jeremy D. , Porciatti Vittorio , Guy John , Yu Hong
Published: December 2023

Leber’s hereditary optic neuropathy (LHON) is a common mitochondrial genetic disease, causing irreversible blindness in young individuals. Current treatments are inadequate, and there is no definitive cure. This study evaluates the effectiveness of delivering wildtype human NADH ubiquinone oxidoreductase subunit 4 (hND4) gene using mito-targeted AAV(MTSAAV) to rescue LHOH mice. We observed a declining pattern in electroretinograms amplitudes as mice aged across all groups (p < 0.001), with significant differences among groups (p = 0.023; Control vs. LHON, p = 0.008; Control vs. Rescue, p = 0.228). Inner retinal thickness and intraocular pressure did not change significantly with age or groups. Compared to LHON mice, those rescued with wildtype hND4 exhibited improved retinal visual acuity (0.29 ± 0.1 cy/deg vs. 0.15 ± 0.1 cy/deg) and increased functional hyperemia response (effect of flicker, p < 0.001, effect of Group, p = 0.004; Interaction Flicker × Group, p < 0.001). Postmortem analysis shows a marked reduction in retinal ganglion cell density in the LHON group compared to the other groups (Effect of Group, p < 0.001, Control vs. LHON, p < 0.001, Control vs. Rescue, p = 0.106). These results suggest that MTSAAV-delivered wildtype hND4 gene rescues, at least in part, visual impairment in an LHON mouse model and has the therapeutic potential to treat this disease.
Predictive Roles of ADAM17 in Patient Survival and Immune Cell Infiltration in Hepatocellular Carcinoma
Authors: Ding Tianlong , Yu Yang , Gao Lei , Xiang Lin , Xu Bo , Gu Baohong , Chen Hao
Published: December 2023

Hepatocellular carcinoma (HCC) is the deadliest malignant tumour worldwide. The metalloproteinase ADAM17 is associated with tumour formation and development; however, its significance in HCC is unclear. This study aimed to investigate the role of ADAM17 in HCC and the correlation between its expression and immune cell infiltration. ADAM17 expression was analysed in pan-cancer and HCC tissues using The Cancer Genome Atlas and Genotype-Tissue Expression datasets. Kaplan–Meier survival analysis displayed a negative association between ADAM17 expression and the overall survival of patients with HCC. High ADAM17 expression was linked to poor tumour/node (T/N) stage and alpha fetoprotein (AFP) levels. Gene Set Enrichment Analysis, Gene Ontology, and Kyoto Encyclopaedia of Genes and Genomes analyses revealed the enrichment of several pathways, including epithelial–mesenchymal transition, inflammatory response, Hedgehog, and KRAS signalling, in patients with upregulated ADAM17. ADAM17 was shown to be positively correlated with immune cell infiltration and immune checkpoint expression via the Tumour Immune Estimation Resource (TIMER) database and immunohistochemistry analyses. Protein–protein interaction (PPI) network analysis revealed that ADAM17 plays a core role in cancer development and immune evasion. In vitro and in vivo experiments demonstrated that ADAM17 influences HCC growth and metastasis. In conclusion, ADAM17 is upregulated in most cancers, particularly HCC, and is critical in the development and immune evasion of HCC.
A Novel Lipase from Streptomyces exfoliatus DSMZ 41693 for Biotechnological Applications
Authors: Rodríguez-Alonso Guillermo , Toledo-Marcos Juan , Serrano-Aguirre Lara , Rumayor Carlos , Pasero Beatriz , Flores Aida , Saborido Ana , Hoyos Pilar , Hernáiz María J. , de la Mata Isabel , Arroyo Miguel
Published: December 2023

Genome mining of Streptomyces exfoliatus DSMZ 41693 has allowed us to identify four different lipase-encoding sequences, and one of them (SeLipC) has been successfully cloned and extracellularly expressed using Rhodococcus sp. T104 as a host. SeLipC was purified by one-step hydrophobic interaction chromatography. The enzyme is a monomeric protein of 27.6 kDa, which belongs to subfamily I.7 of lipolytic enzymes according to its phylogenetic analysis and biochemical characterization. The purified enzyme shows the highest activity at 60 °C and an optimum pH of 8.5, whereas thermal stability is significantly improved when protein concentration is increased, as confirmed by thermal deactivation kinetics, circular dichroism, and differential scanning calorimetry. Enzyme hydrolytic activity using p-nitrophenyl palmitate (pNPP) as substrate can be modulated by different water-miscible organic cosolvents, detergents, and metal ions. Likewise, kinetic parameters for pNPP are: KM = 49.6 µM, kcat = 57 s−1, and kcat/KM = 1.15 × 106 s−1·M−1. SeLipC is also able to hydrolyze olive oil and degrade several polyester-type polymers such as poly(butylene succinate) (PBS), poly(butylene succinate)-co-(butylene adipate) (PBSA), and poly(ε-caprolactone) (PCL). Moreover, SeLipC can catalyze the synthesis of different sugar fatty acid esters by transesterification using vinyl laurate as an acyl donor, demonstrating its interest in different biotechnological applications.
Nephrological Complications in Hemoglobinopathies: SITE Good Practice
Authors: Ruffo Giovan Battista , Russo Rodolfo , Casini Tommaso , Lombardini Letizia , Orecchia Valeria , Voi Vincenzo , Origa Raffaella , Forni Gian Luca , Marchetti Monia , Gigante Antonia , Garibotto Giacomo , Maggio Aurelio , De Franceschi Lucia
Published: December 2023

Background. Hemoglobinopathies, among which thalassemic syndromes (transfusion-dependent and non-transfusion dependent thalassemias) and sickle cell disease (SCD), are the most widespread monogenic diseases worldwide. Hemoglobinopathies are endemic and spread-out all-over Italy, as result of internal and external migration flows. Nowadays, the increase therapeutic options associated to the general aging of patients with hemoglobinopathies related to the improvement in clinical management, contribute to the abnormalities in kidney function going from blood and urine test alterations to chronic kidney disease and end stage renal disease. Methods. Here, we carried out a revision of the literature as panel of recognized experts in hemoglobinopathies with the consultancy and the revision of two nephrologists on kidney alteration and kidney disease in patients with TDT, NTDT and SCD. This is part of the action of the Italian society for the study of thalassemia and hemoglobinopties (SITE). The purpose of this “good practice (GP)” is to provide recommendations for follow-up and therapy for the management of kidney alterations in patients with TDT, NTDT and SCD. The literature review covers the period 1.1.2016 to 31.12.2022. In consideration of the rarity of these diseases, the analysis was extended from 5 to 7 years. Moreover, in the absence of relevant scientific papers in the identified time frame, we referred to pivotal or population studies, when available. Finally, in the absence of evidence-based data from prospective and randomized trials, the authors had to refer to expert opinion (expert consensus) for many topics. Results. We generated question and answer boxes to offer a friendly consultation, using color code strategy and focused answers. Conclusions. The present GP will help in improving the clinical management, and the quality of care of patients with hemoglobinopathies.
Helical Electrodes for Electro-Discharge Drilling: Experimental and CFD-Based Analysis of the Influence of Internal and External Flushing Geometries on the Process Characteristics
Authors: Uhlmann Eckart , Polte Mitchel , Yabroudi Sami , Gerhard Nicklas , Sakharova Ekaterina , Thißen Kai , Penske Wilhelm
Published: December 2023

The electro-discharge (ED) drilling of precision boreholes in difficult-to-machine materials, particularly with respect to the cost-effectiveness of the overall process, is still a challenge. Flushing is one key factor for the precise machining of boreholes, especially with high aspect ratios. Therefore, the influence of internal and external flushing geometries for six types of brass tool electrodes with a diameter of 3 mm with and without a helical groove was analyzed experimentally and numerically. Using this helical external flushing channel, drilling experiments in X170CrVMo18-3-1 (Elmax Superclean) with an aspect ratio of five revealed a material removal rate (MRR) that was increased by 112% compared with a rod electrode, increased by 28% for a single-channel tool electrode and decreased by 8% for a multi-channel tool electrode. Signal analyses complemented these findings and highlighted correlations between classified discharge event types and the experimental target parameters. Amongst others, it was verified that the arcing frequency ratio drove the electrode wear rate and the beneficial frequency ratio correlated with the MRR and the surface roughness Ra. Sophisticated 3D computational fluid dynamics (CFD) models of the liquid phase were introduced and evaluated in great detail to demonstrate the validity and further elucidate the effect of the external flushing channel on the evacuation capability of debris and gas bubbles. The presented methods and models were found to be suitable for obtaining in-depth knowledge about the flushing conditions in the ED drilling working gap.
Loop-Mediated Isothermal Amplification (LAMP): Potential Point-of-Care Testing for Vulvovaginal Candidiasis
Authors: Li Meng , Jin Xiangyu , Jiang Qingyun , Wei Hongbo , Deng Anni , Mao Zeyin , Wang Ying , Zeng Zhen , Wu Yifan , Liu Shuai , Kim Juhyun , Wang Xiaoqian , Liu Ying , Liu Jun , Lv Wenqi , Huang Leyang , Liao Qinping , Huang Guoliang , Zhang Lei
Published: December 2023

Purpose: The aim of this study is to establish a loop-mediated isothermal amplification (LAMP) method for the rapid detection of vulvovaginal candidiasis (VVC). Methods: We developed and validated a loop-mediated isothermal amplification (LAMP) method for detecting the most common Candida species associated with VVC, including C. albicans, N. glabratus, C. tropicalis, and C. parapsilosis. We evaluated the specificity, sensitivity, positive predictive value (PPV), negative predictive value (NPV), and Kappa value of the LAMP method to detect different Candida species, using the conventional culture method and internal transcribed spacer (ITS) sequencing as gold standards and smear Gram staining and real-time Rolymerase Chain Reaction (PCR) as controls. Results: A total of 202 cases were enrolled, of which 88 were VVC-positive and 114 were negative. Among the 88 positive patients, the fungal culture and ITS sequencing results showed that 67 cases (76.14%) were associated with C. albicans, 13 (14.77%) with N. glabratus, 5 (5.68%) with C. tropicalis, and 3 (3.41%) with other species. Regarding the overall detection rate, the LAMP method presented sensitivity, specificity, PPV, NPV, and Kappa values of 90.91%, 100%, 100%, 93.4%, and 0.919, respectively. Moreover, the LAMP had a specificity of 100% for C. albicans, N. glabratus, and C. tropicalis, with a sensitivity of 94.03%, 100%, and 80%, respectively. Moreover, the microscopy evaluation had the highest sensitivity, while the real-time PCR was less specific for C. albicans than LAMP. In addition, CHROMagar Candida was inferior to LAMP in detecting non-albicans Candida (NAC) species. Conclusions: Based on the cost-effective, rapid, and inexpensive characteristics of LAMP, coupled with the high sensitivity and specificity of our VVC-associated Candida detection method, we provided a possibility for the point-of-care testing (POCT) of VVC, especially in developing countries and some laboratories with limited resources.
Myrmecia, Not Asterochloris, Is the Main Photobiont of Cladonia subturgida (Cladoniaceae, Lecanoromycetes)
Authors: Pino-Bodas Raquel , Blázquez Miguel , de los Ríos Asunción , Pérez-Ortega Sergio
Published: December 2023

This study explores the diversity of photobionts associated with the Mediterranean lichen-forming fungus Cladonia subturgida. For this purpose, we sequenced the whole ITS rDNA region by Sanger using a metabarcoding method for ITS2. A total of 41 specimens from Greece, Italy, France, Portugal, and Spain were studied. Additionally, two specimens from Spain were used to generate four cultures. Our molecular studies showed that the genus Myrmecia is the main photobiont of C. subturgida throughout its geographic distribution. This result contrasts with previous studies, which indicated that the main photobiont for most Cladonia species is Asterochloris. The identity of Myrmecia was also confirmed by ultrastructural studies of photobionts within the lichen thalli and cultures. Photobiont cells showed a parietal chloroplast lacking a pyrenoid, which characterizes the species in this genus. Phylogenetic analyses indicate hidden diversity within this genus. The results of amplicon sequencing showed the presence of multiple ASVs in 58.3% of the specimens studied.
Land Snails at the Zoo: A Biodiverse Community with Conservation and Educational Potential
Authors: Bergey Elizabeth A. , Boonmachai Tuangthong
Published: December 2023

The combination of animals and plants in zoological gardens provides the opportunity for conservation within the grounds. An example is pollination gardens that support free-moving butterflies and bees. Pollination gardens and other areas with plants also support a wide range of other non-captive animals, including soil-associated invertebrates. This study targeted land snails, a species-rich group that links the brown decomposition food web and the plant-based web that is preyed upon by fireflies and birds, among others. A survey of 24 sites within the Oklahoma City Zoo and Botanical Garden revealed a diverse snail fauna of 23 species. The majority were native species but included 10 non-native species that are common regional urban species, likely introduced through the plant trade. No distributional pattern of snails was evident, probably because of the similar management scheme throughout the zoo. Snails and other observed soil-associated invertebrates (e.g., isopods, millipedes, and earthworms) support non-captive birds and other animals. Lightly managed areas with leaf litter and downed wood are especially conducive to snails and other soil-associated invertebrates and are nesting sites for bees. These communities can form conservation demonstration areas that can be the basis of educational programs, including outreach programs.
Urban Resilience and Its Links to City Size: Evidence from the Yangtze River Economic Belt in China
Authors: Wang Liang , Li Jingye , Lv Ligang
Published: December 2023

Understanding the relationship between city size and resilience is crucial for informed decisions on preparedness and interventions in building resilient cities. This study addresses this issue by dividing urban resilience into four components: stability, redundancy, resourcefulness, and connectivity. Using the above evaluation framework, we assessed the spatial–temporal variations in the relationship between city size and resilience in the Yangtze River Economic Belt from 2005 to 2020. The findings shows that, overall, resilience increased in the cities, with larger populations and spatial scales showing greater resilience, but both megacities and small cities experienced a decline in resilience. In terms of the four components of resilience, most of the region’s cities have roughly equal connectivity and stability, but redundancy and resourcefulness vary by city size and location. Specifically, downstream and larger cities demonstrated better crisis resolution and innovation. The dominant coupling coordination states showed antagonism between population and resilience. Upstream areas experienced a mismatch between “low resilience” and “large population”, while a moderate coordination existed between spatial scale and resilience. Further, it was found that factors hindering urban resilience varied according to city size. Cities with a population of <3 million faced low connectivity and limited transformation capacity. Those with a population of 3–5 million had moderate connectivity limitations, while cities with a population >5 million faced energy and aging population challenges. This study contributes to urban resilience discourse by providing a conceptual understanding and empirical analysis of the impact of city size on resilience.
Transition Temperature of Color Change in Thermochromic Systems and Its Description Using Sigmoidal Models
Authors: Viková Martina , Vik Michal
Published: December 2023

Background: Symmetric sigmoidal models with four parameters based on an idealized S/Z-shaped curve are commonly used to analyze the optical parameters of thermochromic materials. However, our experimental findings show that this approach leads to systematic errors involving the incorrect estimation of the transition temperature or the possibility of a virtual indication of the hysteresis nature of a reversible thermochromic change. For this reason, we sought to find a five-parameter model that would appropriately avoid this problem. Methods: Two commercial thermochromic pigments were used for the test and applied to a textile substrate at different concentrations. The optical properties were measured using reflectance spectrophotometry and then converted to Kubelka–Munk function values and colorimetric coordinates. The following statistics were used to assess the quality of the selected sigmoidal models: coefficient of determination, R2; adjusted coefficient of determination, AR2; root mean square error, RMSE; and Akaike Information Criterion, AIC. Results: The four-parameter models were compared with each other and with the five-parameter models using nested F-tests based on residual variance to obtain a statistical measure of superior performance. For all thermochromic color change data examined, the five-parameter models resulted in significantly better fitting. It could be shown that the five-parameter model showed significantly higher accuracy and precision in determining the transition temperature, like non-sigmoidal quantification methods. Conclusions: We concluded that the asymmetric five-parameter model is a valuable extension of the symmetric model in the investigation of thermochromic color changes, providing better parameter estimates and a new approach to investigating the mechanisms contributing to the asymmetry of the thermochromic curve.
Radionuclide Removal from Aqueous Solutions Using Oxidized Carbon Fabrics
Authors: Ioannidis Ioannis , Pashalidis Ioannis , Mulla Batuhan , Kotanidis Gkerman , Ioannou Kyriacos , Constantinides Georgios , Kostoglou Nikolaos , Rebholz Claus
Published: December 2023

The adsorption of actinide ions (Am(III) and U(VI)) from aqueous solutions using pristine and oxidized carbon fabrics was investigated by means of batch experiments at different pH values (pH 4, 7 and 9) and temperatures (25, 35 and 45 °C) under ambient atmospheric conditions. The experimental results indicated that both the pH and the fabric texture affected the adsorption rate and the relative removal efficiency, which was 70% and 100% for Am(III) and U(VI), respectively. The Kd (L/kg) values for U(VI) were generally found to be higher (2 < log10(Kd)< 3) than the corresponding values for Am(III) adsorption (1.5 < log10(Kd) < 2). The data obtained from the experiments regarding the temperature effect implied that the relative adsorption for both actinides increases with temperature and that adsorption is an endothermic and entropy-driven reaction. The application of the fabrics to remove the two actinides from contaminated seawater samples showed that both the relative removal efficiency and the Kd values decreased significantly due to the presence of competitive cations (e.g., Ca2+ and Fe3+) and complexing anions (CO32−) in the respective waters. Nevertheless, the removal efficiency was still remarkable (50% and 90% for Am(III) and U(VI), respectively), demonstrating that these materials could be attractive candidates for the treatment of radionuclide/actinide-contaminated waters.
Novel Cr/Si-Slurry Diffusion Coatings for High Temperatures
Authors: Kerbstadt Michael , White Emma Marie Hamilton , Galetz Mathias Christian
Published: December 2023

Surface enrichment in Al, Si, and Cr can greatly improve high temperature oxidation resistance of many alloys. Al, Si, and Cr coatings are commonly applied via simple slurries or more complex pack cementation processes. Due to the high melting point of Cr, the deposition of Cr-based diffusion coatings by the slurry technique has proved challenging, and to date, Cr has mostly been applied by pack cementation. Here, a novel Cr-Si coating process via the slurry technique is described which has been developed and then demonstrated on two Ni-based superalloys, Rene 80 and Inconel 740H. The addition of Si to the slurry lowers the melting point via a Cr-Si eutectic and enables the formation of a liquid phase during heat treatment. Through this Cr-Si slurry coating process diffusion layers enriched by Cr and Si of about 150 µm were achieved. Oxidation behavior was studied through isothermal exposures at 900 °C for 1000 h in lab air. Uncoated Rene 80 and IN740H both showed formation of a Ti-containing Cr2O3 scale below a thin TiO2 top layer. Underneath the external scale a zone of internally oxidized Al grew over the exposure time and reduced the load-bearing cross-section progressively. In comparison, the Cr/Si-coated samples did not show internal Al oxidation, but a slow-growing Si-rich oxide film underneath the external Cr2O3 scale. This subscale represents an additional oxygen diffusion barrier. Thus, the weight gain during exposure for the coated samples was significantly lower than for the uncoated materials.
Composition-Tunable Properties of Cu(Ag) Alloy for Hybrid Bonding Applications
Authors: Singh Sarabjot , Dunn Kathleen
Published: December 2023

In the present study, the properties of Cu(Ag) alloy films were studied to evaluate their potential use as an alternate material for interconnection in hybrid bonding. Thin alloy films of Cu(Ag) were deposited by pulsed electrochemical deposition (PED) using a sulfuric acid-based bath, rotating disk electrode, and hot entry. Secondary ion mass spectrometry (SIMS) was used to measure the silver content of the films, with us finding that it decreases with increasing duty cycle. Thereafter, bright field scanning transmission electron microscope (STEM) imaging in combination with energy-dispersive x-ray spectroscopy (EDS) was used to visualize the thin film microstructure and to confirm the uniform distribution of silver throughout the film, with no bands being seen despite the pulsed nature of the deposition. Film resistance was measured by a four-point probe to quantify the impact of Ag content on resistivity, with us finding the expected linear relationship with the Ag content in the film. Furthermore, the coefficient of thermal expansion (CTE) of the films was measured using X-ray diffraction, and modulus and hardness were measured via nanoindentation, revealing linear dependences on the Ag content as well. Notably, the addition of 1.25 atom% Ag resulted in a significant increase in the CTE from 17.9 to 19.3 ppm/K, Young’s modulus from 111 to 161 GPa, and film hardness from 1.70 to 3.99 GPa. These simple relationships offer a range of properties tunable via the duty cycle of the pulsed plating, making Cu(Ag) a promising candidate for engineering wafer-to-wafer metal interconnections.
Recent Advances in Bromine Complexing Agents for Zinc–Bromine Redox Flow Batteries
Authors: Jiménez-Blasco Uxua , Arrebola José Carlos , Caballero Alvaro
Published: December 2023

The development of energy storage systems (ESS) has become an important area of research due to the need to replace the use of fossil fuels with clean energy. Redox flow batteries (RFBs) provide interesting features, such as the ability to separate the power and battery capacity. This is because the electrolyte tank is located outside the electrochemical cell. Consequently, it is possible to design each battery according to different needs. In this context, zinc–bromine flow batteries (ZBFBs) have shown suitable properties such as raw material availability and low battery cost. To avoid the corrosion and toxicity caused by the free bromine (Br2) generated during the charging process, it is necessary to use bromine complexing agents (BCAs) capable of creating complexes. As an overview, the different BCAs used have been listed to compare their behavior when used in electrolytes in ZBFBs. In addition, the coulombic and energy efficiencies obtained have been compared.
The Influences of Nb Microalloying and Grain Refinement Thermal Cycling on Microstructure and Tribological Properties of Armox 500T
Authors: Youssef Mervat , El-Shenawy Eman H. , Khair-Eldeen Wael , Adachi Tadaharu , Nofal Adel , Hassan Mohsen A.
Published: December 2023

This study aims to investigate the combined effect of niobium (Nb) microalloying and austenite grain refinement, using a specific heat treatment cycle, on the microstructure and tribological properties of Armox 500T steel. In this work, Nb addition and thermal cycling were utilized for grain refinement and enhancement of the mechanical properties of Armox 500T alloy, to provide improved protection via lightweight armor steel components with a high strength-to-weight ratio. The kinetics of transformation of the developed Armox alloys were studied using JMATPro version 13.2. The samples were subjected to two austenitizing temperatures, 1000 °C and 1100 °C, followed by 4 min of holding time and three consecutive thermal and rapid-quenching processes from 900 °C to room temperature. Scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDX) was employed to analyze the microstructure, which primarily consists of four types of martensite: short and long lath martensite, blocky martensite, and equiaxed martensite. Additionally, a small percentage (not exceeding 3%) of carbide precipitates was observed. The wear characteristics of the investigated alloys were evaluated using a pin-on-disc tribometer. The results demonstrate that alloying with Nb and grain refinement using a thermal cycle significantly reduce the wear rate.
Convert Harm into Benefit: The Role of the Al10CaFe2 Phase in Al-Ca Wrought Aluminum Alloys Having High Compatibility with Fe
Authors: Shen Tianying , Zhang Shasha , Liu Zili , Yu Shuaipeng , Jiang Junchao , Tao Xuewei , Akopyan Torgom , Belov Nikolay , Yao Zhengjun
Published: December 2023

The compatibility of the wrought Al-Ca alloy with the element Fe was investigated in the present study. In this work, both the Al-Ca alloy and Al-Ca-Fe alloy were synthesized through melting, casting, heat treatment, and rolling. A new ternary Al-Ca-Fe eutectic phase, identified as Al10CaFe2 with an orthorhombic structure, demonstrated enhanced performance, as revealed by nanoindentation tests. Combining the results of the nanoindentation and EBSD, it can be inferred that during the rolling and heat treatment process, the divorced eutectic phases were broken and spheroidized, and the structure of the Fe-rich alloy became finer, which promotes the formation of fine grains during the process of dynamic recrystallization and effectively hindered the grain growth during thermal treatment. Consequently, the strength of the as-rolled Al-Ca alloy was improved with the addition of 1 wt.% Fe while the ductility of the alloy was maintained. Therefore, adding Ca into the high-Fe content recycled aluminum altered the form of the Fe-containing phases in the alloy, effectively expanding the application scope of recycled aluminum alloy manufacturing. This approach also offered a method for strengthening the Al-Ca aluminum alloys. Compared to the traditional approach of reducing Fe content in alloys through metallurgical means, this study opened a new avenue for designing novel, renewable aluminum alloys highly compatible with impurity iron in scrap.
Construction of Software Supply Chain Threat Portrait Based on Chain Perspective
Authors: Wang Maoyang , Wu Peng , Luo Qin
Published: December 2023

With the rapid growth of the software industry, the software supply chain (SSC) has become the most intricate system in the complete software life cycle, and the security threat situation is becoming increasingly severe. For the description of the SSC, the relevant research mainly focuses on the perspective of developers, lacking a comprehensive understanding of the SSC. This paper proposes a chain portrait framework of the SSC based on a resource perspective, which comprehensively depicts the threat model and threat surface indicator system of the SSC. The portrait model includes an SSC threat model and an SSC threat indicator matrix. The threat model has 3 levels and 32 dimensions and is based on a generative artificial intelligence model. The threat indicator matrix is constructed using the Attack Net model comprising 14-dimensional attack strategies and 113-dimensional attack techniques. The proposed portrait model’s effectiveness is verified through existing SSC security events, domain experts, and event visualization based on security analysis models.
The Optimal Management of Patients with Atrial Fibrillation and Acute Heart Failure in the Emergency Department
Authors: Velliou Maria , Sanidas Elias , Diakantonis Antonis , Ventoulis Ioannis , Parissis John , Polyzogopoulou Effie
Published: December 2023

Atrial fibrillation (AF) and acute heart failure (AHF) are two closely interrelated conditions that frequently coexist in a manifold manner, with AF serving either as the causative factor or as the consequence or even as an innocent bystander. The interplay between these two clinical conditions is complex, given that they share common pathophysiological pathways and they can reciprocally exacerbate each other, thus triggering a vicious cycle that worsens the prognosis and increases the thromboembolic risk. The optimal management of AF in the context of AHF in the emergency department remains a challenge depending on the time onset, as well as the nature and the severity of the associated symptoms. Acute rate control, along with early rhythm control, when indicated, and anticoagulation represent the main pillars of the therapeutic intervention. The purpose of this review is to elucidate the pathophysiological link between AF and AHF and accordingly present a stepwise algorithmic approach for the management of AF in AHF patients in the emergency setting.
The Physics and Manipulation of Dean Vortices in Single- and Two-Phase Flow in Curved Microchannels: A Review
Authors: Saffar Yeganeh , Kashanj Sina , Nobes David S. , Sabbagh Reza
Published: December 2023

Microchannels with curved geometries have been employed for many applications in microfluidic devices in the past decades. The Dean vortices generated in such geometries have been manipulated using different methods to enhance the performance of devices in applications such as mixing, droplet sorting, and particle/cell separation. Understanding the effect of the manipulation method on the Dean vortices in different geometries can provide crucial information to be employed in designing high-efficiency microfluidic devices. In this review, the physics of Dean vortices and the affecting parameters are summarized. Various Dean number calculation methods are collected and represented to minimize the misinterpretation of published information due to the lack of a unified defining formula for the Dean dimensionless number. Consequently, all Dean number values reported in the references are recalculated to the most common method to facilitate comprehension of the phenomena. Based on the converted information gathered from previous numerical and experimental studies, it is concluded that the length of the channel and the channel pathline, e.g., spiral, serpentine, or helix, also affect the flow state. This review also provides a detailed summery on the effect of other geometric parameters, such as cross-section shape, aspect ratio, and radius of curvature, on the Dean vortices’ number and arrangement. Finally, considering the importance of droplet microfluidics, the effect of curved geometry on the shape, trajectory, and internal flow organization of the droplets passing through a curved channel has been reviewed.
A Review of Potential Electrochemical Applications in Buildings for Energy Capture and Storage
Authors: Zhang Jingshi , Azari Rahman , Poerschke Ute , Hall Derek M.
Published: December 2023

The integration of distributed renewable energy technologies (such as building-integrated photovoltaics (BIPV)) into buildings, especially in space-constrained urban areas, offers sustainable energy and helps offset fossil-fuel-related carbon emissions. However, the intermittent nature of these distributed renewable energy sources can negatively impact the larger power grids. Efficient onsite energy storage solutions capable of providing energy continuously can address this challenge. Traditional large-scale energy storage methods like pumped hydro and compressed air energy have limitations due to geography and the need for significant space to be economically viable. In contrast, electrochemical storage methods like batteries offer more space-efficient options, making them well suited for urban contexts. This literature review aims to explore potential substitutes for batteries in the context of solar energy. This review article presents insights and case studies on the integration of electrochemical energy harvesting and storage into buildings. The seamless integration can provide a space-efficient source of renewable energy for new buildings or existing structures that often have limited physical space for retrofitting. This work offers a comprehensive examination of existing research by reviewing the strengths and drawbacks of various technologies for electrochemical energy harvesting and storage, identifying those with the potential to integrate into building skins, and highlighting areas for future research and development.
Versatile Application of TiO2@PDA Modified Filter Paper for Oily Wastewater Treatment
Authors: Zhao Chang-Hua , Zhang Yu-Ping , Wan Li , Chen Xin-Xin , Yuan Pei , Qu Ling-Bo
Published: December 2023

Although membrane separation technology has been widely used in the treatment of oily wastewater, the complexity and high cost of the membrane preparation, as well as its poor stability, limit its further development. In this study, via the vacuum-assisted suction filtration method, polydopamine (PDA)-coated TiO2 nanoparticles were tightly attached and embedded on both sides of laboratory filter paper (FP). The resultant FP possessed the typical wettability of high hydrophilicity in the air with the water contact angle (WCA) of 28°, superoleophilicity with the oil contact angle (OCA) close to 0°, underwater superoleophobicity with the underwater OCA greater than 150°, and superhydrophobicity under the water with the underoil WCA over 150° for five kinds of organic solvents (carbon tetrachloride, toluene, n-hexane, n-octane, and iso-octane). The separation efficiency of immiscible oil/water, oil-in-water, and water-in-oil emulsions using the modified FP is higher than 99%. After 17 cycles of emulsion separation, a high separation efficiency of 99% was still maintained for the FP, along with good chemical and mechanical stability. In addition, successful separation and purification were also realized for the oil-in-water emulsion that contained the methylene blue (MB) dye, along with the complete degradation of MB in an aqueous solution under UV irradiation.
Efficient Selective Capture of Carbon Dioxide from Nitrogen and Methane Using a Metal-Organic Framework-Based Nanotrap
Authors: Peng Junjie , Fu Chengmin , Zhong Jiqin , Ye Bin , Xiao Jing , Duan Chongxiong , Lv Daofei
Published: December 2023

Selective carbon capture from exhaust gas and biogas, which mainly involves the separation of CO2/N2 and CO2/CH4 mixtures, is of paramount importance for environmental and industrial requirements. Herein, we propose an interesting metal-organic framework-based nanotrap, namely ZnAtzCO3 (Atz− = 3-amino-1,2,4-triazolate, CO32− = carbonate), with a favorable ultramicroporous structure and electrostatic interactions that facilitate efficient capture of CO2. The structural composition and stability were verified by FTIR, TGA, and PXRD techniques. Particularly, ZnAtzCO3 demonstrated high CO2 capacity in a wide range of pressures, with values of 44.8 cm3/g at the typical CO2 fraction of the flue gas (15 kPa) and 56.0 cm3/g at the CO2 fraction of the biogas (50 kPa). Moreover, ultrahigh selectivities over CO2/N2 (15:85, v:v) and CO2/CH4 (50:50, v:v) of 3538 and 151 were achieved, respectively. Molecular simulations suggest that the carbon atom of CO2 can form strong electrostatic Cδ+···δ−O-C interactions with four oxygen atoms in the carbonate ligands, while the oxygen atom of CO2 can interact with the hydrogen atoms in the triazolate ligands through Oδ−···δ+H-C interactions, which makes ZnAtzCO3 an optimal nanotrap for CO2 fixation. Furthermore, breakthrough experiments confirmed excellent real-world separation toward CO2/N2 and CO2/CH4 mixtures on ZnAtzCO3, demonstrating its great potential for selective CO2 capture.
Evaluating Gelatin-Based Films with Graphene Nanoparticles for Wound Healing Applications
Authors: Kamedulski Piotr , Wekwejt Marcin , Zasada Lidia , Ronowska Anna , Michno Anna , Chmielniak Dorota , Binkowski Paweł , Łukaszewicz Jerzy P. , Kaczmarek-Szczepańska Beata
Published: December 2023

In this study, gelatin-based films containing graphene nanoparticles were obtained. Nanoparticles were taken from four chosen commercial graphene nanoplatelets with different surface areas, such as 150 m2/g, 300 m2/g, 500 m2/g, and 750 m2/g, obtained in different conditions. Their morphology was observed using SEM with STEM mode; porosity, Raman spectra and elemental analysis were checked; and biological properties, such as hemolysis and cytotoxicity, were evaluated. Then, the selected biocompatible nanoparticles were used as the gelatin film modification with 10% concentration. As a result of solvent evaporation, homogeneous thin films were obtained. The surface’s properties, mechanical strength, antioxidant activity, and water vapor permeation rate were examined to select the appropriate film for biomedical applications. We found that the addition of graphene nanoplatelets had a significant effect on the properties of materials, improving surface roughness, surface free energy, antioxidant activity, tensile strength, and Young’s modulus. For the most favorable candidate for wound dressing applications, we chose a gelatin film containing nanoparticles with a surface area of 500 m2/g.
Beef Intake Is Associated with Higher Nutrient Intake and Nutrient Adequacy in U.S. Adolescents, NHANES 2001–2018
Authors: Fulgoni Kristin , Fulgoni Victor L.
Published: December 2023

Nutrient adequacy among adolescents is of concern due to higher nutrient requirements for their developing bodies as well as the gap between the current nutrient intake and the recommendations. The objective of this study was to determine beef intake and assess the relationship between beef consumption and nutrient intake and nutrient adequacy in male and female adolescents, 14–18 years of age. Dietary recalls collected during the What We Eat in America (WWEIA) portion of the National Health and Nutrition Examination Survey (NHANES) cycles 2001–2018 were utilized to determine beef intake. Usual nutrient intakes were determined with the National Cancer Institute method in conjunction with day 1 and day 2 total nutrient files. Nutrient adequacy was assessed by calculating the percentage of the population below the estimated average requirement (EAR) or above the adequate intake (AI). The average beef intake of male and female adolescent beef consumers was 57.9 ± 2.4 and 46.8 ± 2.2 g with a 90th percentile of 82.3 ± 4.3 and 67.8 ± 3.5 g, respectively. Compared to non-consumers, beef consumers had a 10% or higher intake of calcium, iron, phosphorus, potassium, total choline, vitamin B12, and zinc. Over 50% of the adolescent population (regardless of beef consumption) had intakes below the EAR for calcium, magnesium, and vitamins A, C, D, and E. The percentage of the beef-consuming population below the EAR was lower for calcium, copper, folate, iron, phosphorus, zinc, and vitamins B12 and B6 as compared to non-consumers. Additionally, the portion of the population above the AI for sodium was higher in female beef consumers as compared to non-consumers. We estimate approximately 900,000 to 1,400,000, 400,000–700,000, 200,000–600,000, and 200,000–400,000 fewer adolescents to be below the EAR for zinc, phosphorus, vitamin B12, and iron, respectively if beef non-consumers were to consume beef. This study suggests beef can help increase the nutrient intake and nutrient adequacy in the diets of adolescents, helping to close important gaps for this nutritionally vulnerable population. While recommendations to reduce beef intake are widely prevalent, this could result in unintended nutritional consequences regarding under-consumed nutrients including those of public health concern important for adolescent health.
The Ketogenic Diet in the Prevention of Migraines in the Elderly
Authors: Fila Michal , Chojnacki Jan , Pawlowska Elzbieta , Sobczuk Piotr , Chojnacki Cezary , Blasiak Janusz
Published: December 2023

Migraines display atypical age dependence, as the peak of their prevalence occurs between the ages of 20–40 years. With age, headache attacks occur less frequently and are characterized by a lower amplitude. However, both diagnosis and therapy of migraines in the elderly are challenging due to multiple comorbidities and polypharmacy. Dietary components and eating habits are migraine triggers; therefore, nutrition is a main target in migraine prevention. Several kinds of diets were proposed to prevent migraines, but none are commonly accepted due to inconsistent results obtained in different studies. The ketogenic diet is featured by very low-carbohydrate and high-fat contents. It may replace glucose with ketone bodies as the primary source of energy production. The ketogenic diet and the actions of ketone bodies are considered beneficial in several aspects of health, including migraine prevention, but studies on the ketogenic diet in migraines are not standardized and poorly evidenced. Apart from papers claiming beneficial effects of the ketogenic diet in migraines, several studies have reported that increased levels of ketone bodies may be associated with all-cause and incident heart failure mortality in older adults and are supported by research on mice showing that the ketogenic diets and diet supplementation with a human ketone body precursor may cause life span shortening. Therefore, despite reports showing a beneficial effect of the ketogenic diet in migraines, such a diet requires further studies, including clinical trials, to verify whether it should be recommended in older adults with migraines.