bluuebunny/crossref_metadata_2025 huggingface dataset:
Theoretical Investigation of Origin of Quantized Conduction in 2D Layered Ruddleson–Popper Perovskite Heterostructure for the RRAM Applications
Authors: Rasheed Umbreen , Imran Muhammad , Shakoor Abdul , Niaz Niaz Ahmad , Hussain Fayyaz , Khalil Rana Muhammad Arif , Alkhedher Mohammad , Eldin Sayed M.
Published: December 2022

Quantized conduction achieved in layered materials offers a wide range of applications in electronics. A comprehensive analysis of electronic properties of Sr2ZrO4/TiN- and Sr2ZrO4/TaN-layered heterostructure is carried out using plane wave-based first principles calculations. To understand the origin of quantized conduction, the role of oxygen vacancies (Vos) in 2D layered Ruddleson–Popper perovskite (Sr2ZrO4) is analyzed using density of states, isosurface, and integrated charge density plots. The origin of quantized states formed near the Fermi level is proposed in terms of charge conduction layer formed at the interface. The comprehensive insight of Sr2ZrO4/TiN and Sr2ZrO4/TaN heterostructure interface is provided by shedding light on the charge redistribution from charge density and Bader charge analysis. Meanwhile, work function is calculated for the confirmation of charge conducting behavior of the two layered heterostructures. The interface of these two layered heterostructures revealed the quantized conduction phenomena which cannot be achieved with either layer alone. Stable switching achieved withaTaN electrode being an important task for robust RS and solving sneak path related problem is opening roadmap for 2D layered RRAM devices.
Microencapsulation of Lipases Produced by Dripping and Jet Break-Up for Biodiesel Production
Authors: Guzmán-Martínez Boris , Limas-Ballesteros Roberto , Wang Jin An , Alamilla-Beltrán Liliana , Chen Lifang , Noreña Luis Enrique
Published: December 2022

A high-performance and scalable lipase immobilization method using a dipping and jet break-up technique was reported for the production of microcapsule biocatalysts with an entrapped cascade of lipase enzyme. The lipase from Candida antarctica (CALb) recombinant Aspergillus oryzae and from the vegetal of Jatropha curcas L. (var. Sevangel) in Morelos State of Mexico were entrapped by mixing with a sodium alginate biopolymer at different concentrations. The obtained microcapsules were hardened in a CaCl2 solution, aiming at developing Ca2+ alginate microbeads with sizes mostly from 220 to 300 μm. The relationship between the process variables with the shape and size of the alginate drops before and after the gelation was established with aid of optical image analysis. The results showed that a critical Ohnesorge number (Oh) > 0.24 was required to form spherical microencapsulated beads. The biodiesel production via esterification/transesterification reaction was performed using the crude Jatropha curcas L. oil as feedstock in a batch reactor using lipase microcapsules as biocatalysts. Under the optimal reaction condition (ethanol-to-oil mass ratio: 10; water content 9.1 wt%, microencapsulated biocatalyst mass: 5.25 g, reaction temperature: 35 °C, pH of reaction mixture 7.5, stirring force 6 g), an approximately 95% fatty acid ethyl esters (FAEE) yield could be obtained. The biodiesel obtained from this work completely satisfied with the related ASTM D6751 and EN14214 standards. The microencapsulation technique reported herein allows the production of lipase microcapsules on a continuous large scale with the characteristics required for sustainable biofuel production and it can be also applied in other fields such as food processing and the pharmaceutical industry.
Investigation on the Effects of Magnetic Saturation in Six-Phase Induction Machines with and without Cross Saturation of the Main Flux Path
Authors: Ben Slimene Marwa , Khlifi Mohamed Arbi
Published: December 2022

The operational characteristics during transients are significantly influenced by magnetic saturation in electrical equipment. For the computation of steady-state rated operation in multiphase induction machines, the assumption of linear magnetic behavior of the iron core in classical machine models may be sufficient. The mathematical models of the considered models differ in terms of the existence or absence of dynamic cross-saturation effects. The approach that is most frequently used to examine the impact of magnetic saturation is based on the state-space variable representation of the mathematical model in dynamic axes (d–q). The purpose of this research is to investigate the effects of magnetic saturation on six-phase induction machines. In this study, a d–q transformation-based model of a six-phase induction machine (SPIM), including the magnetic saturation effect, is developed. The cross-saturation and the common mutual leakage inductance between the two sets of stators’ windings are then developed and analyzed, and the developed models were simulated and results are compared with and without cross-saturation. The main and leakage flux saturation, as well as the mutual coupling between the two windings, are all accounted for in the model, which is based on the vector space decomposition method. A significant increase in currents and voltage results from the highly saturated magnetic paths of the leakage fluxes in six-phase induction machines. In order to investigate the impact of cross-saturation, inductances computed using analytical methods and those without taking cross-saturation into consideration were compared. These outcomes are then transformed into a condensed current depending on parameter functions for transient machine models.
Characteristics Analysis of the Pilot-Operated Proportional Directional Valve by Experimental and Numerical Investigation
Authors: Li Shizhen , Du Jiawei , Shi Zilin , Xu Ke , Shi Wenzhuo
Published: December 2022

The main valve spool structure of the pilot-operated proportional directional valve is diverse and has a direct impact on the flow field. To improve the valve’s performance, this work studied the characteristics of four types of spool structures with the following throttling groove arrangements: no throttling groove, a U-shaped groove, a triangle groove, and a combined groove. This study analyzed the flow field simulation of four spool structures under the same opening degree and different pressures to study the flow field cavitation characteristics and pressure distribution in the valve. According to the simulation results, the necessity of opening throttling grooves for the pilot proportional directional valve and the advantages of combined grooves over U-shaped and triangular grooves were verified. Then, the proportional valve with a combined groove structure was simulated and analyzed to study its throttling characteristics, steady flow characteristics, and flow and load differential pressure characteristics, and further explore the advantages of a combined groove. Finally, the experimental results are compared with the simulation results, which can provide a theoretical reference for the selection of the throttle groove of the proportional valve and the structural design of the slide valve.
Simulation-Based Fault Detection Remote Monitoring System for Small-Scale Photovoltaic Systems
Authors: Lim Hee-Won , Kim Il-Kwon , Kim Ji-Hyeon , Shin U-Cheul
Published: December 2022

A small-scale grid-connected PV system that is easy to install and is inexpensive as a remote monitoring system may cause economic losses if its failure is not found and it is left unattended for a long time. Thus, in this study, we developed a low-cost fault detection remote monitoring system for small-scale grid-connected PV systems. This active monitoring system equipped with a simulation-based fault detection algorithm accurately predicts AC power under normal operating conditions and notifies its failure when the measured power is abnormally low. In order to lower the cost, we used a single board computer (SBC) with edge computing as a data server and designed a monitoring system using openHAB, an open-source software. Additionally, we used the Shewhart control chart as a fault detection criterion and the ratio between the measured and predicted ac power for the normal operation data as an observation. As a result of the verification test for the actual grid-connected PV system, it was confirmed that the developed remote monitoring system was able to accurately identify the system failures in real-time, such as open circuit, short circuit, partial shading, etc.
Application of Post-Flotation Dairy Sludge in the Production of Wood Pellets: Pelletization and Combustion Analysis
Authors: Obidziński Sławomir , Joka Yildiz Magdalena , Dąbrowski Sebastian , Jasiński Jan , Czekała Wojciech
Published: December 2022

The amount and variety of waste increases every year. One of the places where biodegradable waste is generated is the agri-food industry, where it is possible to utilize it for the purpose of energy production. The aim of this research was to determine the possibility of using post-floatation dairy sludge as a raw material for co-pelletization with sawdust. The scope of this work included physical and chemical characterizations of the feedstock, the co-pelletization process, and the combustion of the produced pellets, combined with an exhaust analysis. The obtained values of the pellets’ density at each level of sludge addition allowed us to conclude that the obtained pellets had a good market quality and constituted a full-fledged, innovative solid fuel, in accordance with the guidelines of the latest, currently applicable ISO 17225 standard. Furthermore, adding ca. 20%wt of sludge to sawdust resulted in a 30% decrease in the pelletization power demand, and still, the combustion characteristics of the pellets met the European Ecodesign emission limitations in terms of the CO and NOx content in the exhaust. The addition of post-flotation dairy sludge to sawdust has a beneficial influence on the production of fuel pellets by decreasing the energy consumption of the pelletization process and improving the pellets’ kinetic durability. Due to legal requirements and the pursuit of the circular economy principle, one should expect an increased interest in the use of agri-food waste for the production of biofuels.
Ecological Vulnerability in the Red Soil Erosion Area of Changting under Continuous Ecological Restoration: Spatiotemporal Dynamic Evolution and Prediction
Authors: Wu Xinyi , Zhu Chenlu , Yu Junbao , Zhai Lin , Zhang Houxi , Yang Kaijie , Hou Xiaolong
Published: December 2022

Sustainable restoration of degraded ecosystems is a major environmental concern in several regions of China. Changting is one of the severely affected water- and soil-loss areas in southern China that have been under continuous management for the last 30 years. Taking the typical red soil erosion area in Changting, Fujian, as the research object, an evaluation index system with 30 m resolution was developed based on the Sensitivity–Resilience–Pressure (SRP) model. Spatial principal component analysis, Global Moran’s I, the LISA cluster map, and the CA-Markov model were employed to dynamically evaluate and predict the ecological vulnerability of the red soil erosion area in Changting. The findings revealed that the ecological vulnerability of the red soil erosion area in Changting has obvious spatial differences and topography, meteorological, and economic and social variables are the primary driving factors of ecological vulnerability. The analysis of spatial distribution of ecological vulnerability showed significant sets of contiguous locations of severe and mild ecological vulnerability. The total index of ecological vulnerability in the study area reduced by 9.49% from 2000 to 2020, yet it was still just mildly vulnerable. The proportion of severe and extremely vulnerable areas declined by 4.87% and 5.61%, respectively. The prediction results for the coming ten years showed that the ecological vulnerability of red soil erosion in Changting will tend to improve. In summary, it is found that after years of continuous ecological management in the red soil erosion area of Changting, the ecological restoration effect of the soil erosion area is obvious.
Influence of Lactobacillus (LAB) Fermentation on the Enhancement of Branched Chain Amino Acids and Antioxidant Properties in Bran among Wheat By-Products
Authors: Aung Thinzar , Park Seung-Soo , Kim Mi-Jeong
Published: December 2022

The main objective of this study was to enhance the nutritional properties, including branched chain amino acids (BCAAs), through the solid-state fermentation (SSF) of wheat bran (WB) using lactic acid bacteria (LAB). The physicochemical properties, amino acid profiles, bioactive components, and antioxidant properties of raw and sterilized WB were compared with those of WB fermented with five different LAB strains. The highest level of BCAAs, isoleucine (Ile; 2.557 ± 0.05 mg/100 g), leucine (Leu; 7.703 ± 0.40 mg/100 g), and valine (Val; 7.207 ± 0.37 mg/100 g), was displayed in the WB fermented with Lactobacillus acidophilus (L.A WB). In addition, L.A WB showed the highest amount of total phenolic and flavonoid contents (2.80 mg GAE/g and 1.01 mg CE/g, respectively), and the highest Trolox equivalent antioxidant capacity (9.88 mM TE/g). Statistical analysis clearly revealed that L.A WB presented the highest abundance of branched chain amino acids as well as bioactive components. Overall, this study distinctly implemented the possibility of fermented WB with enhanced BCAAs for application in future functional food through experimental and statistical observations.
ERGCN: Enhanced Relational Graph Convolution Network, an Optimization for Entity Prediction Tasks on Temporal Knowledge Graphs
Authors: Wang Yinglin , Xu Xinyu
Published: December 2022

Reasoning on temporal knowledge graphs, which aims to infer new facts from existing knowledge, has attracted extensive attention and in-depth research recently. One of the important tasks of reasoning on temporal knowledge graphs is entity prediction, which focuses on predicting the missing objects in facts at current time step when relevant histories are known. The problem is that, for entity prediction task on temporal knowledge graphs, most previous studies pay attention to aggregating various semantic information from entities but ignore the impact of semantic information from relation types. We believe that relation types is a good supplement for our task and making full use of semantic information of facts can promote the results. Therefore, a framework of Enhanced Relational Graph Convolution Network (ERGCN) is put forward in this paper. Rather than only considering representations of entities, the context semantic information of both relations and entities is considered and merged together in this framework. Experimental results show that the proposed approach outperforms the state-of-the-art methods.
Gene Expression Profiling of Trematomus bernacchii in Response to Thermal and Stabling Stress
Authors: Greco Samuele , Gaetano Anastasia Serena , Furlanis Gael , Capanni Francesca , Manfrin Chiara , Giulianini Piero Giulio , Santovito Gianfranco , Edomi Paolo , Pallavicini Alberto , Gerdol Marco
Published: December 2022

The Antarctic continent is one of the most pristine environments on planet Earth, yet one of the most fragile and susceptible to the effects of the ongoing climate change. The overwhelming majority of the components of Antarctic marine trophic chain are stenotherm organisms, highly adapted to the extreme, but extremely stable, freezing temperatures of the Antarctic ocean, which have not changed significantly during the past fifteen million years. Notothenioid fishes are the most abundant representatives of ichthyofauna at these latitudes, being ubiquitously found in coastal areas across the entire continent. While different Antarctic fish species have been previously subjected to studies aimed at defining their range of thermal tolerance, or at studying the response to acute thermal stress, just a handful of authors have investigated the effects of the exposure to a moderate increase of temperature, falling within the expected forecasts for the next few decades in some areas of the Antarctica. Here, the emerald rockcod Trematomus bernacchii was used as a model species to investigate the effects of a 20-day long exposure to a +1.5 °C increase in the brain, gills and skeletal muscle, using a RNA-sequencing approach. In parallel, the experimental design also allowed for assessing the impact of stabling (including acclimation, the handling of fishes and their confinement in tanks during the experimental phase) on gene expression profiling. The results of this study clearly identified the brain as the most susceptible tissue to heat stress, with evidence of a time-dependent response dominated by an alteration of immune response, protein synthesis and folding, and energy metabolism-related genes. While the gills displayed smaller but still significant alterations, the skeletal muscle was completely unaffected by the experimental conditions. The stabling conditions also had an important impact on gene expression profiles in the brain, suggesting the presence of significant alterations of the fish nervous system, possibly due to the confinement to tanks with limited water volume and of the restricted possibility of movement. Besides providing novel insights in the molecular mechanisms underlying thermal stress in notothenioids, these findings suggest that more attention should be dedicated to an improved design of the experiments carried out on Antarctic organism, due to their extreme susceptibility to the slightest environmental alterations.
Disinfection of Escherichia coli by Mixing with Bulk Ultrafine Bubble Solutions
Authors: Ogata Satoshi , Murata Yuichirou
Published: December 2022

For potential use in wastewater management and health control, this study investigates the disinfection effectiveness of bulk ultrafine bubbles (UFBs) with different bubble number densities and solution pH. Initially, neutral UFB solutions with different bubble concentrations were mixed with E. coli suspension for 120 min, but these solutions did not achieve sterilization. The bubble number density did not affect the disinfection ability of the neutral solution. Next, the pH of the UFB solutions was fixed at 5, 7, and 9. When mixed with E. coli suspension, the acidic UFB solutions reduced the colony counts by 12% after 30 min of cultivation and by 66% after 60 min of cultivation. The colony counts increased slightly in neutral and significantly in alkaline UFB solutions. The acidic UFB solutions had lower zeta potentials and smaller number densities after cultivation, implying that the number density reduced through bubble coalescence rather than increased by bubble collapse. Additionally, the UFBs exhibited insignificant fluorescence intensity, suggesting that the colony counts increased by generated ∙OH radicals. This study revealed that the effect of UFB on E. coli significantly depends on the solution pH. Further, an acidified solvent achieves a bactericidal effect, whereas a neutral or alkaline solvent enhances the growth effect. This result is important when using actual wastewater.
Rent Dissipation in Simple Tullock Contests
Authors: Dickson Alex , MacKenzie Ian A. , Sekeris Petros G.
Published: December 2022

We investigate observed rent dissipation—the ratio of the total costs of rent seeking to the monetary value of the rent—in winner-take-all and share contests, where preferences are more general than usually assumed in the literature. With concave valuation of the rent, we find that contests can exhibit observed over-dissipation if the contested rent is below a threshold and yet observed under-dissipation with large rents: the nature of preferences implies contestants are relatively effortful in contesting small rents. Considering more general preferences in contests thus allows us to reconcile the Tullock paradox—where rent-seeking levels are relatively small despite the contested rent being sizeable—with observed over-dissipation of rents in experimental settings, where contested rents are arguably small.
Introduction to Electroweak Baryogenesis
Authors: Gannouji Radouane
Published: December 2022

We present a pedagogical introduction to the electroweak baryogenesis. The review focuses principally on the sphaleron and baryon number (non)-conservation or chiral anomaly. All results are derived with details for a self-contained reading.
PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations
Authors: Cui Pengfei , Ma Luping , Jiang Pengju , Wang Cheng , Wang Jianhao
Published: December 2022

Currently, nucleic acid preparations have gained much attention due to their unique working principle and application value. However, as macromolecular drugs, nucleic acid preparations have complex construction and poor stability. The current methods to promote stability face problems such as high cost and inconvenient operatios. In this study, the hydrophilic pharmaceutical excipient PEG was used to gelate nucleic acid preparations to avoid the random movements of liquid particles. The results showed that PEG gelation significantly improved the stability of PEI25K−based and liposome−based nucleic acid preparations, compared with nucleic acid preparations without PEG gelation. After being stored at 4 °C for 3 days, non−PEG gelled nucleic acid preparations almost lost transfection activity, while PEGylated preparations still maintained high transfection efficiency. Fluorescence experiments showed that this effect was caused by inhibiting particle aggregation. The method described in this study was simple and effective, and the materials used had good biocompatibility. It is believed that this study will contribute to the better development of gene therapy drugs.
Xerogels Morphology Details by Multifractal Analysis and Scanning Electron Microscopy Images Evaluations of 5-Fluorouracil Release from Chitosan-Based Matrix
Authors: Paun Maria-Alexandra , Nichita Mihai-Virgil , Paun Vladimir-Alexandru , Paun Viorel-Puiu
Published: December 2022

Four medicament delivery formulations based on 5-fluorouracil in a chitosan substantial matrix were realized in situ via 3,7-dimethyl-2,6-octadienal element hydrogelation. Representative samples of the final realized compounds were investigated from an analytic, constitutional, and morphological viewpoint via Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The SEM images of the formulations were investigated in concordance with fractal analysis, and the fractal dimensions and lacunarity were computed. The developed mathematical multifractal model is necessarily confirmed by the experimental measurements corresponding to the 5-fluorouracil release outside the chitosan-formed matrix.
Challenges in Gene Therapy for Somatic Reverted Mosaicism in X-Linked Combined Immunodeficiency by CRISPR/Cas9 and Prime Editing
Authors: Hou Yujuan , Ureña-Bailén Guillermo , Mohammadian Gol Tahereh , Gratz Paul Gerhard , Gratz Hans Peter , Roig-Merino Alicia , Antony Justin S. , Lamsfus-Calle Andrés , Daniel-Moreno Alberto , Handgretinger Rupert , Mezger Markus
Published: December 2022

X-linked severe combined immunodeficiency (X-SCID) is a primary immunodeficiency that is caused by mutations in the interleukin-2 receptor gamma (IL2RG) gene. Some patients present atypical X-SCID with mild clinical symptoms due to somatic revertant mosaicism. CRISPR/Cas9 and prime editing are two advanced genome editing tools that paved the way for treating immune deficiency diseases. Prime editing overcomes the limitations of the CRISPR/Cas9 system, as it does not need to induce double-strand breaks (DSBs) or exogenous donor DNA templates to modify the genome. Here, we applied CRISPR/Cas9 with single-stranded oligodeoxynucleotides (ssODNs) and prime editing methods to generate an in vitro model of the disease in K–562 cells and healthy donors’ T cells for the c. 458T>C point mutation in the IL2RG gene, which also resulted in a useful way to optimize the gene correction approach for subsequent experiments in patients’ cells. Both methods proved to be successful and were able to induce the mutation of up to 31% of treated K–562 cells and 26% of treated T cells. We also applied similar strategies to correct the IL2RG c. 458T>C mutation in patient T cells that carry the mutation with revertant somatic mosaicism. However, both methods failed to increase the frequency of the wild-type sequence in the mosaic T cells of patients due to limited in vitro proliferation of mutant cells and the presence of somatic reversion. To the best of our knowledge, this is the first attempt to treat mosaic cells from atypical X-SCID patients employing CRISPR/Cas9 and prime editing. We showed that prime editing can be applied to the formation of specific-point IL2RG mutations without inducing nonspecific on-target modifications. We hypothesize that the feasibility of the nucleotide substitution of the IL2RG gene using gene therapy, especially prime editing, could provide an alternative strategy to treat X-SCID patients without revertant mutations, and further technological improvements need to be developed to correct somatic mosaicism mutations.
Breeding and Selection of Nursery Plants Assisted by High-Throughput Field Phenotyping Using UAV Imagery: Case Studies with Sweet Box (Sarcococca) and Garden Rose (Rosa)
Authors: Borra-Serrano Irene , Van Laere Katrijn , Lootens Peter , Leus Leen
Published: December 2022

Breeding and selection of nursery plants require evaluation of a wide variety of traits. Characteristics that are visually scored in the field include aesthetic beauty as well as tolerance to (a)biotic stresses, among others. This work proposes methodologies based on vegetation indices and canopy height data derived from visual imagery captured using an RGB (red, green, blue) camera embedded in a drone to rank and select genotypes. This approach relies on quantitative evaluation standards that exclude breeder bias and speed up data collection. A proof of concept for nursery plants was developed in two woody ornamentals: sweet box (Sarcococca Lindl.) and garden rose (Rosa L.). This work aimed to compare methodologies and to propose how drones (unmanned aerial vehicles, UAV) and high throughput field phenotyping (HTFP) can be used in a commercial plant selection program. Data was gathered in 2019 in three well-established breeding trials, two for sweet box and one for rose. Characteristics discussed include plant architecture traits such as plant height and shape in the sweet box and rose as well as floribundity, continuous flowering and disease resistance in roses. Correlations were calculated between on-ground measurements and UAV-derived methods, obtaining significant results. The advantages and disadvantages of the methodology and the approach for each of the traits are discussed.
Quality and Yield of Bell Pepper Cultivated with Two and Three Stems in a Modern Agriculture System
Authors: Flores-Velazquez Jorge , Mendoza-Perez Cándido , Rubiños-Panta Juan Enrique , Ruelas-Islas Jesus del Rosario
Published: December 2022

Bell pepper is a very important crop for its value in domestic and foreign markets. Actually, growers have adopted different management practices. In that aspect, management with different numbers of stems can define the quality and quantity of the product, as well as any increase in yield. The objective of this work was to evaluate the physical and chemical characteristics of fruits in terms of the quality and postharvest of bell pepper, as well as the yield according to the number of stems grown in a hydroponic system under greenhouse conditions. The experiment consisted of four treatments: two stems (T1) and three stems (T2) on a ‘Cannon’ cultivar, as well as two stems (T3) and three stems (T4) on a ‘Bragi’ cultivar. Fruits were sampled to determine total soluble solids (TSS), titratable acidity (TA), pH, electrical conductivity (EC), maturity index (MI), vitamin C (VC), lycopene content, size, shape, color, firmness, and yield. High vitamin C concentration of 120 mg 100 g−1 was found in both cultivars. Treatments from ‘Cannon’ had the higher TSS content, lycopene levels and firmness. Regarding the physical characteristics, T1 of ‘Cannon’ had better fruit size: 63% (large), 35% (medium) and 2% (small). The highest yield was obtained in T2 of ‘Bragi’ with 6.50 kg m−2. It was observed that total number of fruits increased as the number of stems increased. However, the size of the fruits decreased.
Prognosis and Survival in Idiopathic Pulmonary Fibrosis in the Era of Antifibrotic Therapy in Italy: Evidence from a Longitudinal Population Study Based on Healthcare Utilization Databases
Authors: Iommi Marica , Faragalli Andrea , Bonifazi Martina , Mei Federico , Latini Lara Letizia , Pompili Marco , Carle Flavia , Gesuita Rosaria
Published: December 2022

The aim was to evaluate the determinants of acute exacerbation (AE) and death in new cases of idiopathic pulmonary fibrosis (IPF) using administrative databases in the Marche Region. Adults at their first prescription of antifibrotics or hospitalization with a diagnosis of IPF occurring in 2014–2019 were considered as new cases. Multiple Cox regression was used to estimate the risk of AE and of all-cause mortality adjusted by demographic and clinical characteristics, stratifying patients according to antifibrotic treatment. Overall, 676 new cases of IPF were identified and 276 deaths and 248 AE events occurred. In never-treated patients, the risk of AE was higher in patients with poor health conditions at diagnosis; the risk of death was higher in males, in patients aged ≥75 and in those with poor health conditions at baseline. The increasing number of AEs increased the risk of death in treated and never-treated patients. Within the limits of an observational study based on secondary data, the combined use of healthcare administrative databases allows the accurate analysis of progression and survival of IPF from the beginning of the antifibrotic therapy era, suggesting that timely and early diagnosis is critical to prescribing the most suitable treatment to increase survival and maintain a healthy life expectancy.
Factors Related to Anxiety in Paediatric Patients and Their Parents before and after a Modified Ravitch Procedure—A Single-Centre Cohort Study
Authors: Fenikowski Dariusz , Tomaszek Lucyna
Published: December 2022

Objective. To assess the factors related to perioperative state anxiety in paediatric patients and their parents. Methods. A cohort study was conducted on paediatric patients 9–17 years of age, who underwent the modified Ravitch procedure (n = 96), and their parents (n = 96). The level of anxiety was measured using the State-Trait Anxiety Inventory questionnaire. Multivariable linear regression models were calculated to find the relationships between the pre- and postoperative state anxiety of the patients/parents and the independent variables, both demographic (age, gender) and clinical (e.g., postoperative pain, trait anxiety). Results. Preoperative anxiety in the paediatric patients was positively correlated with their trait anxiety (β = 0.47; 95% CI: 0.29 to 0.64) and preoperative parental anxiety (β = 0.24; 95% CI: 0.07 to 0.42). The high level of preoperative anxiety (vs. low and moderate) (β = 0.40; 95% CI: 0.22 to 0.58), trait anxiety (β = 0.22; 95% CI: 0.04 to 0.40) and average postoperative pain at rest (β = 0.18; 95% CI: 0.01 to 0.34) had a positive impact on the postoperative anxiety in patients. However, the patients’ age was negatively correlated with postoperative anxiety (β = −0.19; 95% CI: −0.35 to −0.02). Three variables were found to predict preoperative parental anxiety: their trait anxiety (β = 0.41; 95% CI: 0.23 to 0.59), female gender (β = 0.18; 95% CI: 0.002 to 0.36) and the intravenous route for the postoperative pain management in the patients (β = −0.18; 95% CI: −0.36 to −0.001). The parental postoperative anxiety was influenced by their trait anxiety (β = 0.24; 95% CI: 0.04 to 0.43), preoperative anxiety in patients (β = 0.21; 95% CI: 0.02 to 0.40) and female gender of children (β = 0.19; 95% CI: 0.001 to 0.39). Conclusions. Trait anxiety was a strong factor positively affecting the perioperative state anxiety. In addition, paediatric patient anxiety before surgery was related to their parents’ anxiety, and, after surgery, this was associated with high preoperative anxiety, pain and age. The parents’ anxiety before surgery was influenced by gender and the type of postoperative analgesia in the patients, while, after surgery, this was influenced by the patients’ preoperative anxiety/gender.
Parental Psychological Control and Addiction Behaviors in Smartphone and Internet: The Mediating Role of Shyness among Adolescents
Authors: Zhang Qi , Ran Guangming , Ren Jing
Published: December 2022

Parental psychological control has been found to be a vital familial factor that is closely related to adolescents’ addiction behaviors with regard to smartphones and the internet. However, the underlying mechanisms of these associations are less clear. The aim of the present study was to examine whether shyness mediated the relationships between parental psychological control and these two addiction behaviors. A positivist paradigm was used in the present study. The questionnaires (parental psychological control, shyness, and smartphone and internet addiction questionnaires) were used to collect data from a sample of 1857 Chinese adolescents (961 female, 896 male) in junior and senior middle schools. Descriptive statistics as well as correlation and mediation tests were employed to analyze the data. We observed that adolescents with siblings showed a higher level of internet addiction than those with no siblings. Moreover, three dimensions of parental psychological control were positively associated with addiction behaviors. The following analyses displayed that the correlation between authority assertion and smartphone addiction was greater than that between authority assertion and internet addiction. Subsequently, shyness was significantly positively related to parental psychological control and addiction behaviors. Importantly, we found that the relations between the three dimensions of parental psychological control and the addiction behaviors concerning smartphones and the internet were partially mediated by shyness. This study contributes to our understanding of how parental psychological control predicts high levels of adolescents’ addiction behaviors surrounding smartphones and the internet.
Carbon Footprint of Away-From-Home Food Consumption in Brazilian Diet
Authors: Bezerra Ilana Nogueira , Verde Sara Maria Moreira Lima , Almeida Bruno de Sousa , de Azevedo Clarisse Vasconcelos
Published: December 2022

Environmentally unsustainable diets are often characterized by being high in calories, processed foods, and red meats, characteristics related to away-from-home food (AFHF). The aim of this study is to evaluate if AFHF consumption is related to environmental sustainability. Data of 20,780 adults from 24 h recalls collected in the 2017–2018 Brazilian National Dietary Survey (NDS) were used to estimate carbon footprint coefficients. The mean carbon footprint was estimated among individuals who consumed AFHF and non-consumers. Linear regression models were used to evaluate differences between away-from-home eating and the carbon footprint of the diet, adjusting for age and income. A total of 41% of Brazilians consumed AFHF during the previous day. The mean carbon footprint from foods consumed away from home represented 18% of the total carbon footprint. AFHF was positively associated with increased total carbon footprint (β: 204.1; p-value: 0.0145). In conclusion, the consumption of foods away from home in urban areas of Brazil was associated with atmospheric greenhouse gas emissions independently of age and income. Away-from-home food consumption should be considered to reinforce the influence of diet on individual and planet health.
SARS-CoV-2 Seroconversion and Pregnancy Outcomes in a Population of Pregnant Women Recruited in Milan, Italy, between April 2020 and October 2020
Authors: Accurti Veronica , Gambitta Bianca , Iodice Simona , Manenti Alessandro , Boito Simona , Dapporto Francesca , Leonardi Margherita , Molesti Eleonora , Fabietti Isabella , Montomoli Emanuele , Bollati Valentina , Persico Nicola
Published: December 2022

The possible link between SARS-CoV-2 infection and adverse pregnancy outcomes has so far demonstrated heterogeneous results in terms of maternal, fetal, and neonatal complications. We aim to investigate the correlation between SARS-CoV-2 seroconversion and/or neutralization titer and pregnancy outcomes. We analyzed a population of 528 pregnant women followed up from the first trimester of gestation until delivery. For each woman, we collected a first blood sample between 11 and 13 weeks of gestation and a second sample in the perinatal period (between peripartum and puerperium) to assess the presence of SARS-CoV-2 antibodies and/or microneutralization titer (MN titer). Data on pregnancy outcomes (gestational age at delivery, preterm birth before 34 weeks, hypertensive disorders, gestational diabetes, and abnormal fetal growth) were collected. We observed that serologic status per se is not associated with major pregnancy complications. On the contrary, the MN titer was associated with increased odds of gestational diabetes. Although we mainly reported asymptomatic SARS-CoV-2 infections and the absence of severe maternal and neonatal adverse outcomes, SARS-CoV-2 infection might challenge the maternal immune system and explain the moderate increase in adverse outcome odds.
Antiherpetic Activity of Carrageenan Complex with Echinochrome A and Its Liposomal Form
Authors: Krylova Natalia V. , Gorbach Vladimir I. , Iunikhina Olga V. , Pott Anastasia B. , Glazunov Valery P. , Kravchenko Anna O. , Shchelkanov Mikhail Y. , Yermak Irina M.
Published: December 2022

Herpes simplex virus (HSV) infections, the incidence of which is still widespread throughout the world, are actualizing the search and development of new, more effective antiherpetic drugs. The development of multifunctional drug delivery systems, including liposome-based ones, has become a relevant and attractive concept in nanotechnology. The ability of complexes of κ- and Σ-carrageenans (CRGs)—sulfated polysaccharides of red algae, with echinochrome A (Ech), as well as the liposomal form of the Σ-CRG/Ech complex—to inhibit different stages of HSV-1 infection in Vero cells was studied. By quantum chemical calculations, it was shown that CRG forms stable complexes with Ech. We have shown that complexes of κ-CRG/Ech and Σ-CRG/Ech exhibit highest virucidal activity with a selectivity index (SI) of 270 and 350, respectively, and inhibition of virus-cell interaction (SI of 83 and 32, respectively). The liposomal form of the Σ-CRG/Ech complex after virus adsorption and penetration to cells effectively reduced the HSV-1 plaque formation. The virus-inhibiting activity of the liposomal form of the Σ-CRG/Ech complex was three times higher than that of the Σ-CRG/Ech complex itself. Obtaining CRGs/Ech complexes and their liposomal forms can become the basis of a successful strategy for the development of promising antiherpetic drugs.
When Two Maladies Meet: Disease Burden and Pathophysiology of Stroke in Cancer
Authors: Sun Ming-Yee , Bhaskar Sonu M. M.
Published: December 2022

Stroke and cancer are disabling diseases with an enormous global burden, disproportionately affecting vulnerable populations and low- and middle-income countries. Both these diseases share common risk factors, which warrant concerted attention toward reshaping population health approaches and the conducting of fundamental studies. In this article, an overview of epidemiological trends in the prevalence and burden of cancer and stroke, underlying biological mechanisms and clinical risk factors, and various tools available for risk prediction and prognosis are provided. Finally, future recommendations for research and existing gaps in our understanding of pathophysiology. Further research must investigate the causes that predispose patients to an increased risk of stroke and/or cancer, as well as biomarkers that can be used to predict growing morbidity and mortality.
Transcriptome and Methylome Profiling in Rat Skeletal Muscle: Impact of Post-Weaning Protein Restriction
Authors: Ma Sihui , Hasegawa Emi , Nakai Yuji , Jia Huijuan , Kato Hisanori
Published: December 2022

Skeletal muscle is programmable, and early-life nutritional stimuli may form epigenetic memory in the skeletal muscle, thus impacting adult muscle function, aging, and longevity. In the present study, we designed a one-month protein restriction model using post-weaning rats, followed by a two-month rebound feeding, to investigate how early-life protein restriction affects overall body growth and muscle development and whether these influences could be corrected by rebound feeding. We observed comprehensive alterations immediately after protein restriction, including retarded growth, altered biochemical indices, and disturbed hormone secretion. Transcriptome profiling of the gastrocnemius muscle followed by gene ontology analyses revealed that “myogenic differentiation functions” were upregulated, while “protein catabolism” was downregulated as a compensatory mechanism, with enhanced endoplasmic reticulum stress and undesired apoptosis. Furthermore, methylome profiling of the gastrocnemius muscle showed that protein restriction altered the methylation of apoptotic and hormone secretion-related genes. Although most of the alterations were reversed after rebound feeding, 17 genes, most of which play roles during muscle development, remained altered at the transcriptional level. In summary, early-life protein restriction may undermine muscle function in the long term and affect skeletal muscle development at the both transcriptional and methylation levels, which may hazard future muscle health.
Susceptibility of Human Plasma N-glycome to Low-Calorie and Different Weight-Maintenance Diets
Authors: Deriš Helena , Tominac Petra , Vučković Frano , Astrup Arne , Blaak Ellen E. , Lauc Gordan , Gudelj Ivan
Published: December 2022

Aberrant plasma protein glycosylation is associated with a wide range of diseases, including diabetes, cardiovascular, and immunological disorders. To investigate plasma protein glycosylation alterations due to weight loss and successive weight-maintenance diets, 1850 glycomes from participants of the Diogenes study were analyzed using Ultra-High-Performance Liquid Chromatography (UHPLC). The Diogenes study is a large dietary intervention study in which participants were subjected to a low-calorie diet (LCD) followed by one of five different weight-maintenance diets in a period of 6 months. The most notable alterations of the plasma glycome were 8 weeks after the subjects engaged in the LCD; a significant increase in low-branched glycan structures, accompanied by a decrease in high-branched glycan structures. After the LCD period, there was also a significant rise in N-glycan structures with antennary fucose. Interestingly, we did not observe significant changes between different diets, and almost all effects we observed immediately after the LCD period were annulled during the weight-maintenance diets period.
N-Heterocyclic Carbenes and Their Metal Complexes Based on Histidine and Histamine Derivatives of Bacteriopurpurinimide for the Combined Chemo- and Photodynamic Therapy of Cancer
Authors: Tikhonov Sergey , Morozova Natalia , Plutinskaya Anna , Plotnikova Ekaterina , Pankratov Andrey , Abramova Olga , Diachkova Ekaterina , Vasil’ev Yuriy , Grin Mikhail
Published: December 2022

Photodynamic therapy (PDT) is currently regarded as a promising method for the treatment of oncological diseases. However, it involves a number of limitations related to the specific features of the method and the specific characteristics of photosensitizer molecules, including tumor hypoxia, small depth of light penetration into the tumor tissue, and low accumulation sensitivity. These drawbacks can be overcome by combining PDT with other treatment methods, for example, chemotherapy. In this work, we were the first to obtain agents that contain bacteriopurpurinimide as a photodynamic subunit and complexes of gold(I) that implement the chemotherapy effect. To bind the latter agents, N-heterocyclic carbenes (NHC) based on histidine and histamine were obtained. We considered alternative techniques for synthesizing the target conjugates and selected an optimal one that enabled the production of preparative amounts for biological assays. In vitro studies showed that all the compounds obtained exhibited high photoinduced activity. The C-donor Au(I) complexes exhibited the maximum specific activity at longer incubation times compared to the other derivatives, both under exposure to light and without irradiation. In in vivo studies, the presence of histamine in the NHC-derivative of dipropoxy-BPI (7b) had no significant effect on its antitumor action, whereas the Au(I) metal complex of histamine NHC-derivative with BPI (8b) resulted in enhanced antitumor activity and in an increased number of remissions after photodynamic treatment.
The NF-Y Transcription Factor Family in Watermelon: Re-Characterization, Assembly of ClNF-Y Complexes, Hormone- and Pathogen-Inducible Expression and Putative Functions in Disease Resistance
Authors: Jiang Siyu , Wang Hui , Wen Ya , Liang Jiayu , Li Dayong , Song Fengming
Published: December 2022

Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor that binds to the CCAAT cis-element in the promoters of target genes and plays critical roles in plant growth, development, and stress responses. In the present study, we aimed to re-characterize the ClNF-Y family in watermelon, examine the assembly of ClNF-Y complexes, and explore their possible involvement in disease resistance. A total of 25 ClNF-Y genes (7 ClNF-YAs, 10 ClNF-YBs, and 8 ClNF-YCs) were identified in the watermelon genome. The ClNF-Y family was comprehensively characterized in terms of gene and protein structures, phylogenetic relationships, and evolution events. Different types of cis-elements responsible for plant growth and development, phytohormones, and/or stress responses were identified in the promoters of the ClNF-Y genes. ClNF-YAs and ClNF-YCs were mainly localized in the nucleus, while most of the ClNF-YBs were localized in the cytoplasm of cells. ClNF-YB5, -YB6, -YB7, -YB8, -YB9, and -YB10 interacted with ClNF-YC2, -YC3, -YC4, -YC5, -YC6, -YC7, and -YC8, while ClNF-YB1 and -YB3 interacted with ClNF-YC1. A total of 37 putative ClNF-Y complexes were identified, e.g., ClNF-YA1, -YA2, -YA3, and -YA7 assembled into 13, 8, 8, and 8 ClNF-Y complexes with different ClNF-YB/-YC heterodimers. Most of the ClNF-Y genes responded with distinct expression patterns to defense hormones such as salicylic acid, methyl jasmonate, abscisic acid, and ethylene precursor 1-aminocyclopropane-1-carboxylate, and to infection by the vascular infecting fungus Fusarium oxysporum f. sp. niveum. Overexpression of ClNF-YB1, -YB8, -YB9, ClNF-YC2, and -YC7 in transgenic Arabidopsis resulted in an earlier flowering phenotype. Overexpression of ClNF-YB8 in Arabidopsis led to enhanced resistance while overexpression of ClNF-YA2 and -YC2 resulted in decreased resistance against Botrytis cinerea. Similarly, overexpression of ClNF-YA3, -YB1, and -YC4 strengthened resistance while overexpression of ClNF-YA2 and -YB8 attenuated resistance against Pseudomonas syringae pv. tomato DC3000. The re-characterization of the ClNF-Y family provides a basis from which to investigate the biological functions of ClNF-Y genes in respect of growth, development, and stress response in watermelon, and the identification of the functions of some ClNF-Y genes in disease resistance enables further exploration of the molecular mechanism of ClNF-Ys in the regulation of watermelon immunity against diverse pathogens.