bluuebunny/crossref_metadata_2025 huggingface dataset:
Biocidal Activity of Phyto-Derivative Products Used on Phototrophic Biofilms Growing on Stone Surfaces of the Domus Aurea in Rome (Italy)
Authors: Rugnini Lorenza , Migliore Giada , Tasso Flavia , Ellwood Neil Thomas William , Sprocati Anna Rosa , Bruno Laura
Published: September 2020

Hypogean or enclosed monuments are important cultural heritage sites that can suffer biodegradation. Many of the stone walls of the prestigious Domus Aurea are overwhelmed by dense biofilms and so need intervention. Room 93 was chosen as a study site with the aim to test the efficacy of phyto-derivatives as new biocides. Laboratory studies were performed comparing the effects of liquorice leaf extract (Glycyrrhiza glabra L.), lavender essential oil (Lavandula angustifolia Mill.) and a combination of both. In situ studies were also performed to test the effect of liquorice. The results were compared with those of the commonly used synthetic biocide benzalkonium chloride. The effects on the biofilms were assessed by microscopy along with chlorophyll fluorescence analysis. The phototrophs in the biofilms were identified morphologically, while the heterotrophs were identified with culture analysis and 16S gene sequencing. Results showed that the mixed solution liquorice/lavender was the most effective in inhibiting the photosynthetic activities of biofilms in the laboratory tests; while, in situ, the effect of liquorice was particularly encouraging as an efficient and low-invasive biocide. The results demonstrate a high potential biocidal efficacy of the phyto-derivatives, but also highlight the need to develop an efficient application regime.
Reduction of Stress Concentration Factor (SCF) on the Bolted Joint Connection for a Large Wind Turbine Rotor Blade through Various Design Modifications
Authors: Ha Kwangtae
Published: September 2020

The importance of a reliable blade root connection has grown due to the higher-gravity-induced edgewise loads on the blade root that resulted from the recent increased size and weight of a wind turbine rotor blade. To avoid the loosening of a bolt joint connection or even consecutive blade failures, the stress concentration factor (SCF) at the bolt thread root that is sensitive to fatigue should be understood comprehensively. In this work, two-dimensional and three-dimensional finite element (FE) analysis methods were used to determine the SCF at the bolt threads both between an insert and a M42 bolt used for a large offshore blade, and between a M42 bolt and a nut. The effect of various geometric parameters on the SCF were also investigated, which included shank diameter, nut height, nut type, and relief cone. Results showed that the decreased diameter of a M42 bolt shank diameter was the dominant design driver in reducing the stress concentration factor by 40%, from 3.94 to 2.32. The round nut type was also a recommended factor to be implemented to connect bolts and inner pitch bearing with an additional 10% SCF reduction. The relief cones applied to bolt threads and insert threads also contributed to the reduction of SCF to 2.01, a 49% reduction in total. This work not only provides guidelines by which to choose the proper geometry of the bolt and nut for a large blade, but also could be beneficial in designing bolted joint connections of segment or modular blades.
Natural Gas as a New Prospect in Everyday Use of Electric Vehicles
Authors: Prodromidis George N. , Mytakis Dennis E. , Coutelieris Frank A.
Published: September 2020

We study here the urban use of electric vehicles (EVs), focusing on the electricity production for charging purposes. This work proposes an innovative charging scheme for EVs, by introducing a home-applied power station, consisting of a fuel cell combined with an internal reforming unit, which is fed by natural gas and can thus be directly connected to the already-established natural gas grid. We therefore overcome the barriers posed by hydrogen use (establishment of storage equipment, energy consumption for keeping high pressure, development of supply grid, etc.) while we eliminate the environmental impact, since no fossil fuels are required for electricity production. Furthermore, comparisons against EVs charged by grid and vehicles fueled by petrol, both subjected to urban everyday use, have been carried out here. Precisely, we financially compare the use of an EV charged directly through the national electric grid against our innovative power station. Both options are also compared with the same vehicle, powered by an internal combustion engine fueled by petrol. This study also implements a detailed thermodynamic analysis for this state-of-the-art power station and an additional financial analysis for the everyday use of these vehicles under the three different scenarios. For the sake of equivalence, the new Peugeot 208 was selected as the reference-vehicle, as it is equipped either with a petrol engine or an electric motor, under a roughly identical performance profile. This work also introduces the use of the existing grid of natural gas to produce the necessary electricity for charging EVs, rather than using other renewables (solar, wind, etc.), and could further strengthen the worldwide acceptance of the EVs as a viable and a financially feasible solution for everyday urban transportation.
Observational Practices for Urban Microclimates Using Meteorologically Instrumented Unmanned Aircraft Systems
Authors: Adkins Kevin , Wambolt Peter , Sescu Adrian , Swinford Christopher , Macchiarella Nickolas D.
Published: September 2020

The urban boundary layer (UBL) is one of the most important and least understood atmospheric domains and, consequently, warrants deep understanding and rigorous analysis via sophisticated experimental and numerical tools. When field experiments have been undertaken, they have primarily been accomplished with either a coarse network of in-situ sensors or slow response sensors based on timing or Doppler shifts, resulting in low resolution and decreasing performance with height. Small unmanned aircraft systems (UASs) offer an opportunity to improve on traditional UBL observational strategies that may require substantive infrastructure or prove impractical in a vibrant city, prohibitively expensive, or coarse in resolution. Multirotor UASs are compact, have the ability to take-off and land vertically, hover for long periods of time, and maneuver easily in all three spatial dimensions, making them advantageous for probing an obstacle-laden environment. Fixed-wing UASs offer an opportunity to cover vast horizontal and vertical distances, at low altitudes, in a continuous manner with high spatial resolution. Hence, fixed-wing UASs are advantageous for observing the roughness sublayer above the highest building height where traditional manned aircraft cannot safely fly. This work presents a methodology for UBL investigations using meteorologically instrumented UASs and discusses lessons learned and best practices garnered from a proof of concept field campaign that focused on the urban canopy layer and roughness sublayer of a large modern city with a high-rise urban canopy.
Inner-Shell Photodetachment of Na− Using R-Matrix Methods
Authors: Gorczyca T. W. , Zhou H.-L. , Hibbert A. , Hasoglu M. F. , Manson S. T.
Published: September 2020

Inner-shell photodetachment of Na− near the L-edge threshold was investigated using the R-matrix method. Significant structure was found in the cross section, and this structure is shown to be related to the complicated correlated electron dynamics endemic in negative ions. Comparison with experiment suggests that the absolute values of the measured cross section might be too small by a factor of two.
Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats
Authors: Rehman Muneeb U. , Rashid Summya , Arafah Azher , Qamar Wajhul , Alsaffar Rana M. , Ahmad Ajaz , Almatroudi Nada M. , Alqahtani Saeed M. A. , Rashid Shahzada Mudasir , Ahmad Sheikh Bilal
Published: September 2020

Colon cancer is the most common cancer in men and women globally, killing millions of people annually. Though there widespread development has been made in the management of colorectal cancer, still there is an urgent need to find novel targets for its effective treatment. Piperine is an alkaloid found in black pepper having anticancer, anti-inflammatory activities, safe and nutritive for human consumption. Nuclear factor-erythroid 2–kelch-like ECH-associated protein 1(Nrf-2/Keap-1)/Heme-oxygenase1 (HO-1) signaling pathway plays a vital part in shielding cells from intracellular oxidative stress and inflammation. A potential cross-talk between the Nrf-2 and NF-κB pathways is recognized during cancerous growth and expansion. We studied this pathway extensively in the present study to discover novel targets in the prevention of chemically induced colon cancer with piperine to simulate human colon cancer pathology. Animals were divided into four groups. Groups1 and 2 were used as a negative control and positive control where 1,2–Dimethylhydrazine, DMH was administered in group 2, while group 3 and 4 were prevention groups where piperine at two different doses was given two weeks prior to DMH and continued until end of experiment. We found that piperine inhibited NF-κB by the activation of Nrf-2, blocking downstream inflammatory mediators/cytokines (TNF-α, IL-6, IL-1β, Cox-2, PGE-2, iNOS, NO, MPO), triggering an antioxidant response machinery (HO-1, NQO-1, GSH, GR, GPx, CAT, SOD), scavenging ROS, and decreasing lipid peroxidation. Histological findings further validated our molecular findings. It also downregulates CEA, MDF and ACF, markers of precancerous lesions in colon, alleviates infiltration of mast cells and depletes the mucous layer. Our results indicate that piperine may be an effective molecule for the prophylactic treatment of colon carcinogenesis by targeting the NF-κB/Nrf-2/Keap-1/HO-1 pathway as a progressive strategy in the preclusion and effective treatment of colorectal cancer.
Short Bouts of Gait Data and Body-Worn Inertial Sensors Can Provide Reliable Measures of Spatiotemporal Gait Parameters from Bilateral Gait Data for Persons with Multiple Sclerosis
Authors: Motti Ader Lilian Genaro , Greene Barry R. , McManus Killian , Tubridy Niall , Caulfield Brian
Published: September 2020

Wearable devices equipped with inertial sensors enable objective gait assessment for persons with multiple sclerosis (MS), with potential use in ambulatory care or home and community-based assessments. However, gait data collected in non-controlled settings are often fragmented and may not provide enough information for reliable measures. This paper evaluates a novel approach to (1) determine the effects of the length of the walking task on the reliability of calculated measures and (2) identify digital biomarkers for gait assessments from fragmented data. Thirty-seven participants (37) diagnosed with relapsing-remitting MS (EDSS range 0 to 4.5) executed two trials, walking 20 m each, with inertial sensors attached to their right and left shanks. Gait events were identified from the medio-lateral angular velocity, and short bouts of gait data were extracted from each trial, with lengths varying from 3 to 9 gait cycles. Intraclass correlation coefficients (ICCs) evaluate the degree of agreement between the two trials of each participant, according to the number of gait cycles included in the analysis. Results show that short bouts of gait data, including at least six gait cycles of bilateral data, can provide reliable gait measurements for persons with MS, opening new perspectives for gait assessment using fragmented data (e.g., wearable devices, community assessments). Stride time variability and asymmetry, as well as stride velocity variability and asymmetry, should be further explored as digital biomarkers to support the monitoring of symptoms of persons with neurological diseases.
Resveratrol Prevents GLUT3 Up-Regulation Induced by Middle Cerebral Artery Occlusion
Authors: Gutiérrez Aguilar Germán Fernando , Alquisiras-Burgos Iván , Franco-Pérez Javier , Pineda-Ramírez Narayana , Ortiz-Plata Alma , Torres Ismael , Pedraza-Chaverri José , Aguilera Penélope
Published: September 2020

Glucose transporter (GLUT)3 up-regulation is an adaptive response activated to prevent cellular damage when brain metabolic energy is reduced. Resveratrol is a natural polyphenol with anti-oxidant and anti-inflammatory features that protects neurons against damage induced in cerebral ischemia. Since transcription factors sensitive to oxidative stress and inflammation modulate GLUT3 expression, the purpose of this work was to assess the effect of resveratrol on GLUT3 expression levels after ischemia. Male Wistar rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) followed by different times of reperfusion. Resveratrol (1.9 mg/kg; i. p.) was administered at the onset of the restoration of the blood flow. Quantitative-PCR and Western blot showed that MCAO provoked a substantial increase in GLUT3 expression in the ipsilateral side to the lesion of the cerebral cortex. Immunofluorescence assays indicated that GLUT3 levels were upregulated in astrocytes. Additionally, an important increase in GLUT3 occurred in other cellular types (e.g., damaged neurons, microglia, or infiltrated macrophages). Immunodetection of the microtubule-associated protein 2 (MAP2) showed that MCAO induced severe damage to the neuronal population. However, the administration of resveratrol at the time of reperfusion resulted in injury reduction. Resveratrol also prevented the MCAO-induced increase of GLUT3 expression. In conclusion, resveratrol protects neurons from damage induced by ischemia and prevents GLUT3 upregulation in the damaged brain that might depend on AMPK activation.
Nitrogen Doped Superactivated Carbons Prepared at Mild Conditions as Electrodes for Supercapacitors in Organic Electrolyte
Authors: Mostazo-López María José , Ruiz-Rosas Ramiro , Tagaya Tomomi , Hatakeyama Yoshikiyo , Shiraishi Soshi , Morallón Emilia , Cazorla-Amorós Diego
Published: September 2020

Nitrogen functionalization of a highly microporous activated carbon (SBET > 3000 m2/g), to be used as electrode of electric double layer capacitor (EDLC), was carried out by different methods based on organic chemistry protocols at low temperature and selective thermal post-treatments under inert atmosphere. The combination of both methods allowed the production of carbon materials with very similar surface area (2400–3000 m2/g) and different surface chemistry. The nitrogen functionalization by chemical methods produce the attachment of 4 at. % N (XPS) by consumption of oxygen functional groups. The thermal treatments rearrange the surface chemistry by decreasing and converting both nitrogen and oxygen moieties. The effect of surface chemistry on the performance of these materials as electrodes for symmetric supercapacitors was analyzed in organic electrolyte (1M TEMABF4/propylene carbonate). The devices showed high gravimetric capacitance (37–40 F/g) and gravimetric energy density (31–37 Wh/kg). The electrochemical stability of the EDLC was evaluated by a floating test under severe conditions of voltage and temperature. The results evidence an improvement of the durability of nitrogen-doped activated carbons modified by chemical treatments due to the decrease of detrimental oxygen functionalities and the generation of nitrogen groups with higher electrochemical stability.
Nitrogen-Doped Arginine Carbon Dots and Its Metal Nanoparticle Composites as Antibacterial Agent
Authors: Suner Selin S. , Sahiner Mehtap , Ayyala Ramesh S. , Bhethanabotla Venkat R. , Sahiner Nurettin
Published: September 2020

Nitrogen (N)-doped arginine carbon dots (Arg CD) were successfully synthesized using arginine as the amine source and citric acid as the carbon source via a one-pot green synthesis microwave-assisted technique in 2 min. Ag and Cu nanoparticles (NP) were generated within N-doped Arg CDs as composite Arg-Ag CDs and Arg-Cu CDs to render enhanced antibacterial properties. TEM analysis revealed that Arg CDs are in graphitic structures with d spacing ranging from 0.5 nm to 10 nm. The minimum inhibition concentration (MIC) values of Arg CDs with 6.250 mg/mL were decreased by about 100-fold for Arg-Ag CDs and ten-fold for Arg-Cu CDs with 0.062 and 0.625 mg/mL MIC values against Staphylococcus aureus (S. aureus). The highest antibacterial susceptibility was observed for the Arg-Ag CD composite with 0.125 and 0.312 mg/mL minimum bactericidal concentration (MBC) values against Gram negative S. aureus and Gram positive Escherichia coli (E. coli) bacteria strains, respectively. It was found that the metal NPs within Arg CDs significantly increased the antibacterial properties of CDs making them available in the treatment of infections caused by different bacterial species. Furthermore, Arg-Ag CD and Arg-Cu CD composites were tested for Acetylcholinesterase (AChE, E.C. 3.1.1.7) that break down acetylcholine (ACh) into choline and acetic acid leading to the loss of ACh which plays an essential role as neurotransmitter in Alzheimer disease. It was found that Arg-Cu CDs inhibited 74.9 ± 0.8% and Arg-Ag CDs inhibited 52.1 ± 3.8% of AChE at a 1.82 mg/mL concentration versus no inhibition for Arg-CD. Moreover, the chelating activity of Arg-Cu CDs and Arg-Ag CDs were tested for Fe(II) and it was found that almost 100% chelating was attained at 116 μg composites versus no measurable chelation for bare Arg CDs, suggesting the potential neurodegenerative disease treatment properties of these composite CDs in the brain.
Secreting Germ Cell Tumors of the Central Nervous System: A Long-Term Follow-up Experience
Authors: Biassoni Veronica , Schiavello Elisabetta , Gandola Lorenza , Pecori Emilia , Poggi Geraldina , Spreafico Filippo , Terenziani Monica , Meazza Cristina , Podda Marta , Ferrari Andrea , Luksch Roberto , Casanova Michela , Puma Nadia , Chiaravalli Stefano , Bergamaschi Luca , Cefalo Graziella , Simonetti Fabio , Gattuso Giovanna , Seregni Ettore Cesare , Pallotti Federica , Gianno Francesca , Diletto Barbara , Barretta Francesco , Massimino Maura
Published: September 2020

Introduction: Due to the rarity of nongerminomatous germ cell tumors (NGGCT) with non-standard treatment as yet, we report retrospectively our 30 year experience with chemotherapy followed by craniospinal irradiation (CSI), plus a boost of whole ventricular irradiation (WVI)/tumor bed (TB), tailored to pre-radiation chemotherapy response. Methods: Between 1988 and 2016, 28 patients received four cycles of PEB (cisplatin/etoposide/bleomycin), then CSI, and two further PEB cycles. Between 1988 and1994, CSI was 25.5 Gy for patients in complete remission (CR), 30 Gy if in partial remission (PR) or metastatic, with a boost to TB up to 45–54 Gy. In the period of 1995–2010, the boost included WVI and any extra-ventricular tumor sites up to 45 Gy. After 2010, CSI was reduced to 25.5 Gy for all non-metastatic patients, and a boost was given only to TB up to 40.5/45.5 Gy, depending on patients’ CR/PR status. After 2003, patients with alfafetoprotein (αFP) > 1000 ng/mL received intensified treatment, also including autologous stem cell transplantation. Results: Among 28 patients (23 males; median age 12 years, 6 metastatic), 25 responded to PEB, and three progressed (PD) after one to four cycles; 26 received radiotherapy obtaining 13 CR, 7 PR and 5 stable disease (SD), 1 PD; 6 (21%) died (5 for disease, 1 for pneumonia while in CR). Five-year overall survival (OS) and progression-free survival (PFS) were both 81%; 10 year OS and PFS 81% and 76%, respectively (median follow-up 11 years). Conclusions: Survival for children with NGGCT, independently from disease extent, was encouraging. Further studies should elucidate which patients could benefit from reduced volume and dose irradiation.
Immunotherapy Breakthroughs in the Treatment of Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma
Authors: Borel Christian , Jung Alain C. , Burgy Mickaël
Published: September 2020

Head and neck squamous cell carcinoma (HNSCC) in the recurrent or metastatic (R/M) setting is a devastating disease with a poor prognosis. Until recently, the reference first line treatment was the EXTREME protocol, which yields a 10.1 months median survival, and almost no effective treatment are available in second line. Immune checkpoint inhibitors (ICIs) have changed the prognosis of several metastatic solid tumors. Given their inflammatory profile and high mutational burden, HNSCC is a good candidate for ICIs treatments. First, a strong pembrolizumab efficacy signal was shown in the Keynote-012 Phase Ib study. Then, the phase III Checkmate-141 study validated the efficacy of nivolumab in platinum-resistant patients. Finally, the first line conquest is acquired since the final results of the keynote-048 phase III study that demonstrated the superiority of pembrolizumab versus EXTREME in CPS ≥ 1 patients, and with the addition of platinum and 5FU in all patients. However, the first line treatment landscape is not frozen. Two studies (Checkmate-651 and Kestrel) are investigating the efficacy of the combination of antibodies raised against CTLA-4 and PD-(L)1. Results are impatiently awaited. Further progress needs the use of new immunotherapeutic agents such as monalizumab or ICOS agonist rather in combination with an anti-PD(L)1. New associations of ICIs and chemotherapeutic or targeted therapeutic agents are also actively investigated. Finally, ICIs has to be studied in the locally advanced setting where there is a chance of cure. Several trials are testing the potential synergistic combination of ICIs with radiotherapy and platinum or cetuximab, or ICIs used in a neoadjuvant setting.
Small Molecule Inhibitors of Microenvironmental Wnt/β-Catenin Signaling Enhance the Chemosensitivity of Acute Myeloid Leukemia
Authors: Takam Kamga Paul , Dal Collo Giada , Cassaro Adriana , Bazzoni Riccardo , Delfino Pietro , Adamo Annalisa , Bonato Alice , Carbone Carmine , Tanasi Ilaria , Bonifacio Massimiliano , Krampera Mauro
Published: September 2020

Wnt/β-catenin signaling has been reported in Acute Myeloid leukemia, but little is known about its significance as a prognostic biomarker and drug target. In this study, we first evaluated the correlation between expression levels of Wnt molecules and clinical outcome. Then, we studied—in vitro and in vivo—the anti-leukemic value of combinatorial treatment between Wnt inhibitors and classic anti-leukemia drugs. Higher levels of β-catenin, Ser675-phospho-β-catenin and GSK-3α (total and Ser 9) were found in AML cells from intermediate or poor risk patients; nevertheless, patients presenting high activity of Wnt/β-catenin displayed shorter progression-free survival (PFS) according to univariate analysis. In vitro, many pharmacological inhibitors of Wnt signalling, i.e., LRP6 (Niclosamide), GSK-3 (LiCl, AR-A014418), and TCF/LEF (PNU-74654) but not Porcupine (IWP-2), significantly reduced proliferation and improved the drug sensitivity of AML cells cultured alone or in the presence of bone marrow stromal cells. In vivo, PNU-74654, Niclosamide and LiCl administration significantly reduced the bone marrow leukemic burden acting synergistically with Ara-C, thus improving mouse survival. Overall, our study demonstrates the antileukemic role of Wnt/β-catenin inhibition that may represent a potential new therapeutics strategy in AML.
Structural Disorder of Mechanically Activated δ-MgCl2 Studied by Synchrotron X-ray Total Scattering and Vibrational Spectroscopy
Authors: Wada Toru , Thakur Ashutosh , Chammingkwan Patchanee , Terano Minoru , Taniike Toshiaki , Piovano Alessandro , Groppo Elena
Published: September 2020

A combination of synchrotron X-ray total scattering and molecular simulation is a powerful approach for reliable determination of the structure of δ-MgCl2 as an indispensable component of heterogeneous Ziegler–Natta catalysts. Here, the same approach is applied to mechanically activated MgCl2. Four types of mechanically activated MgCl2 samples are prepared using ball-milling in the absence and presence of different donors. The development of structural disorder along the grinding time is compared. It was found that the presence of donors accelerates the formation of δ-MgCl2 in an early stage of grinding, while elongated grinding eventually results in δ-MgCl2 with similar extents of structural disorder in the absence and presence of different donors. The FT-IR investigation consistently verified the morphological similarity between the firmly ground samples. Thus, the structure of δ-MgCl2 is likely governed by mechanical energy when sufficiently ground.
Antiviral Effect of Visible Light-Sensitive CuxO/TiO2 Photocatalyst
Authors: Miyauchi Masahiro , Sunada Kayano , Hashimoto Kazuhito
Published: September 2020

Photocatalysis is an effective technology for preventing the spread of pandemic-scale viruses. This review paper presents an overview of the recent progress in the development of an efficient visible light-sensitive photocatalyst, i.e., a copper oxide nanoclusters grafted titanium dioxide (CuxO/TiO2). The antiviral CuxO/TiO2 photocatalyst is functionalised by a different mechanism in addition to the photocatalytic oxidation process. The CuxO nanocluster consists of the valence states of Cu(I) and Cu(II); herein, the Cu(I) species denaturalizes the protein of the virus, thereby resulting in significant antiviral properties even under dark conditions. Moreover, the Cu(II) species in the CuxO nanocluster serves as an electron acceptor through photo-induced interfacial charge transfer, which leads to the formation of an anti-virus Cu(I) species and holes with strong oxidation power in the valence band of TiO2 under visible-light irradiation. The antiviral function of the CuxO/TiO2 photocatalyst is maintained under indoor conditions, where light illumination is enabled during the day but not during the night; this is because the remaining active Cu(I) species works under dark conditions. The CuxO/TiO2 photocatalyst can thus be used to reduce the risk of virus infection by acting as an antiviral coating material.
Coupling Pre-Reforming and Partial Oxidation for LPG Conversion to Syngas
Authors: Potemkin Dmitriy I. , Rogozhnikov Vladimir N. , Uskov Sergey I. , Shilov Vladislav A. , Snytnikov Pavel V. , Sobyanin Vladimir A.
Published: September 2020

Coupling of the pre-reforming and partial oxidation was considered for the conversion of liquefied petroleum gas to syngas for the feeding applications of solid oxide fuel cells. Compared with conventional two step steam reforming, it allows the amount of water required for the process, and therefore the energy needed for water evaporation, to be lowered; substitution of high-potential heat by lower ones; and substitution of expensive tubular steam reforming reactors by adiabatic ones. The supposed process is more productive due to the high reaction rate of partial oxidation. The obtained syngas contains only ca. 10 vol.% H2O and ca. 50 vol.% of H2 + CO, which is attractive for the feeding application of solid oxide fuel cells. Compared with direct partial oxidation of liquefied petroleum gas, the suggested scheme is more energy efficient and overcomes problems with coke formation and catalyst overheating. The proof-of-concept experiments were carried out. The granular Ni-Cr2O3-Al2O3 catalyst was shown to be effective for propane pre-reforming at 350–400 °C, H2O:C molar ratio of 1.0, and flow rate of 12,000 h−1. The composite Rh/Ce0.75Zr0.25O2-δ–ƞ-Al2O3/FeCrAl catalyst was shown to be active and stable under conditions of partial oxidation of methane-rich syngas after pre-reforming and provided a syngas (H2 + CO) productivity of 28 m3·Lcat−1·h−1 (standard temperature and pressure).
Schwann Cell Role in Selectivity of Nerve Regeneration
Authors: Bolívar Sara , Navarro Xavier , Udina Esther
Published: September 2020

Peripheral nerve injuries result in the loss of the motor, sensory and autonomic functions of the denervated segments of the body. Neurons can regenerate after peripheral axotomy, but inaccuracy in reinnervation causes a permanent loss of function that impairs complete recovery. Thus, understanding how regenerating axons respond to their environment and direct their growth is essential to improve the functional outcome of patients with nerve lesions. Schwann cells (SCs) play a crucial role in the regeneration process, but little is known about their contribution to specific reinnervation. Here, we review the mechanisms by which SCs can differentially influence the regeneration of motor and sensory axons. Mature SCs express modality-specific phenotypes that have been associated with the promotion of selective regeneration. These include molecular markers, such as L2/HNK-1 carbohydrate, which is differentially expressed in motor and sensory SCs, or the neurotrophic profile after denervation, which differs remarkably between SC modalities. Other important factors include several molecules implicated in axon-SC interaction. This cell–cell communication through adhesion (e.g., polysialic acid) and inhibitory molecules (e.g., MAG) contributes to guiding growing axons to their targets. As many of these factors can be modulated, further research will allow the design of new strategies to improve functional recovery after peripheral nerve injuries.
Association between Exercise-Induced Changes in Cardiorespiratory Fitness and Adiposity among Overweight and Obese Youth: A Meta-Analysis and Meta-Regression Analysis
Authors: García-Hermoso Antonio , Izquierdo Mikel , Alonso-Martínez Alicia M. , Faigenbaum Avery , Olloquequi Jordi , Ramírez-Vélez Robinson
Published: September 2020

The aim of this study was to determine the minimum change in cardiorespiratory fitness (CRF) required to reduce adiposity (percent body fat) in exercise programs for overweight and obese youth. Studies were identified through a systematic search of five databases. Studies were limited to randomized controlled trials (RCTs) of exercise training (e.g., aerobic, strength, concurrent) that assessed percent body fat and CRF for both exercise and control groups in overweight and obese children and adolescents. A series of meta-regressions were conducted to explore links between change in CRF (maximum oxygen consumption, ml/kg/min) and change in percent body fat. Twenty-three RCTs were included (n = 1790, 59% females). Meta-regression analysis suggested that increases of at least 0.38 mL/kg/min in CRF (p < 0.001) were considered to be a clinically important reduction of percent body fat (−2.30%, 95% confidence interval −3.02 to −1.58; p < 0.001; I2 = 92.2%). Subgroup analysis showed that increases of at least 0.17 mL/kg/min in CRF favored a reduction of percent body fat of −1.62% (95% confidence interval −2.04 to −1.20; p < 0.001; I2 = 69.9%). In conclusion, this change in CRF could be considered by pediatric researchers, youth fitness specialists, and health care providers to determine the effectiveness in body fat reductions through exercise.
Fabrication of Fe‒Al Coatings with Micro/Nanostructures for Antifouling Applications
Authors: He Zhaorong , Wang Dacheng , Fan Zhiqing , Chen Yingjun , Li Shidong , Mo Caisong
Published: September 2020

Fouling is one of the common problems in heat-transfer applications, resulting in higher fouling resistance, and lower heat-transfer coefficient. This paper introduces the design and fabrication of an Fe–Al coating with micro/nanostructures on low-carbon steel by electrical discharge coating (EDC) technology to improve the antifouling property. The Fe–Al coating with micro/nanostructures is characterized by a large number of micro/nanostructures and superior anti-fouling property, which is attributed to its hydrophobic surface. The antifouling property, fouling induction period and contact angle of the Fe–Al coating with micro/nanostructures increase with the increasing gap voltage. Compared with the polished surface of low-carbon steel, the Fe–Al coating with micro/nanostructures extends the induction period from 214 to 1350 min, with a heat flux of 98 kW·m−2. After 50 adhesion tests, the contact angle of the Fe–Al coating with micro/nanostructures decreases from 6.81% to 27.52%, which indicates that the Fe–Al coating with micro/nanostructures is durable and suitable for industrial applications.
A Graphics Process Unit-Based Multiple-Relaxation-Time Lattice Boltzmann Simulation of Non-Newtonian Fluid Flows in a Backward Facing Step
Authors: Molla Md. Mamun , Nag Preetom , Thohura Sharaban , Khan Amirul
Published: September 2020

A modified power-law (MPL) viscosity model of non-Newtonian fluid flow has been used for the multiple-relaxation-time (MRT) lattice Boltzmann methods (LBM) and then validated with the benchmark problems using the graphics process unit (GPU) parallel computing via Compute Unified Device Architecture (CUDA) C platform. The MPL model for characterizing the non-Newtonian behavior is an empirical correlation that considers the Newtonian behavior of a non-Newtonian fluid at a very low and high shear rate. A new time unit parameter (λ) governing the flow has been identified, and this parameter is the consequence of the induced length scale introduced by the power law. The MPL model is free from any singularities due to the very low or even zero shear-rate. The proposed MPL model was first validated for the benchmark study of the lid-driven cavity and channel flows. The model was then applied for shear-thinning and shear-thickening fluid flows through a backward-facing step with relatively low Reynolds numbers, Re = 100–400. In the case of shear-thinning fluids (n=0.5), laminar to transitional flow arises while Re≥300, and the large vortex breaks into several small vortices. The numerical results are presented regarding the velocity distribution, streamlines, and the lengths of the reattachment points.
Fog Computing for Realizing Smart Neighborhoods in Smart Grids
Authors: Jaiswal Rituka , Davidrajuh Reggie , Rong Chunming
Published: September 2020

Cloud Computing provides on-demand computing services like software, networking, storage, analytics, and intelligence over the Internet (“the cloud”). But it is facing challenges because of the explosion of the Internet of Things (IoT) devices and the volume, variety, veracity and velocity of the data generated by these devices. There is a need for ultra-low latency, reliable service along with security and privacy. Fog Computing is a promising solution to overcome these challenges. The originality, scope and novelty of this paper is the definition and formulation of the problem of smart neighborhoods in context of smart grids. This is achieved through an extensive literature study, firstly on Fog Computing and its foundation technologies, its applications and the literature review of Fog Computing research in various application domains. Thereafter, we introduce smart grid and community MicroGrid concepts and, their challenges to give the in depth background of the problem and hence, formalize the problem. The smart grid, which ensures reliable, secure, and cost-effective power supply to the smart neighborhoods, effectively needs Fog Computing architecture to achieve its purpose. This paper also identifies, without rigorous analysis, potential solutions to address the problem of smart neighborhoods. The challenges in the integration of Fog Computing and smart grids are also discussed.
Salivary Alpha-Amylase in Experimentally-Induced Muscle Pain
Authors: Christidis Nikolaos , Baghernejad Pegah , Deyhim Aylin , Jasim Hajer
Published: September 2020

Salivary alpha-amylase (sAA) is a marker of psychological stress and might also be a potential marker for pain-associated stress due its non-invasive, cost-effective, and stress-free collection. The current study aimed to investigate whether the levels of sAA are influenced by experimentally induced muscle pain. In this study, 26 healthy, pain-free and age-matched participants (23.8 ± 2.6 years) were included, 13 women and 13 men. Prior to the experiment, questionnaires assessing health and anxiety were completed. Muscle pain was then induced through intramuscular injection of 0.4 mL hypertonic saline (56.5 mg/mL) into the masseter muscle and unstimulated whole saliva samples were collected at baseline before injection, 2 min, and 15 min after injection. A commercially available colorimetric assay was used to analyze the sAA. Perceived pain and stress were assessed using a 0–100 Numeric Rating Scale for each sample. There were no significant differences in sAA levels prior and after injection of hypertonic saline (p > 0.05) although sAA levels showed a slight decrease during experimentally-induced muscle pain. However, a strong correlation was observed between self-reported pain and perceived level of stress during experimentally-induced muscle pain (r2 = 0.744; p < 0.0001). Furthermore, there was a moderate correlation between the levels of sAA at baseline and during experimental pain (r2 = 0.687; p < 0.0001). In conclusion, this study could not show any association between the levels of sAA and perceived pain and or/stress. However, since a significant strong correlation could be observed between perceived stress and pain intensity, this study indicates that experimentally-induced muscle pain could be used as a stress model.
Biomarker Utility for Peripheral Artery Disease Diagnosis in Real Clinical Practice: A Prospective Study
Authors: Ceasovschih Alexandr , Sorodoc Victorita , Onofrei (Aursulesei) Viviana , Tesloianu Dan , Tuchilus Cristina , Anisie Ecaterina , Petris Antoniu , Statescu Cristian , Jaba Elisabeta , Stoica Alexandra , Grigorescu Elena-Daniela , Jaba Irina M. , Sorodoc Laurentiu
Published: September 2020

Peripheral arterial disease (PAD) is a common manifestation of generalized atherosclerosis, which affects more than 200 million patients worldwide. Currently, there is no ideal biomarker for PAD risk stratification and diagnosis. The goal of this research was to investigate the levels of inflammation biomarkers and cystatin C and to explore their utility for the diagnosis of PAD. The study included 296 participants, distributed in two groups: 216 patients diagnosed with PAD and 80 patients without PAD as controls. All studied biomarker levels (C-reactive protein, CRP; fibrinogen; erythrocyte sedimentation rate, ESR; neopterin; beta 2-microglobulin, B2-MG; and cystatin C) were significantly higher in the PAD group and indirectly correlated with the ankle–brachial index (ABI). The final logistic regression model included an association of neopterin, fibrinogen, and cystatin C as the most efficient markers for the prediction of PAD diagnosis. When comparing the area under the curve (AUC) for all biomarkers, the value for neopterin was significantly higher than those of all the other analyzed biomarkers. In agreement with previous studies, this research shows that markers such as fibrinogen, CRP, ESR, B2-MG, and cystatin C have significant value for the diagnosis of PAD, and also clearly underlines the accuracy of neopterin as a leading biomarker in PAD prediction.
Outcomes of Atrioseptostomy with Stenting in Patients with Pulmonary Arterial Hypertension from a Large Single-Institution Cohort
Authors: Gorbachevsky Sergey V. , Shmalts Anton A. , Dadabaev Gulomjon M. , Nishonov Nasirullo A. , Pursanov Manolis G. , Shvartz Vladimir A. , Zaets Sergey B.
Published: September 2020

The aim of this study was to analyze results of stenting atrioseptostomy in patients with pulmonary arterial hypertension and a different level of risk for one-year mortality that is not well described. Patients that underwent atrioseptostomy with stenting were retrospectively divided in two groups: “intermediate” (n = 55) or “high” risk (n = 13), according to the 2015 ESC/ESR guideline. Results of atrioseptostomy were assessed during hospital period and at follow-up. Patients from “intermediate” risk group demonstrated lower mortality rate (10/55, vs. 6/13) during the course of the study period, as well as higher freedom from lung transplantation or Potts shunt. At discharge, patients of both groups presented improvement in functional class and mobility. Patients from “intermediate” risk group showed longer 6-min walking distance, and lower levels of brain natriuretic peptide. At the latest follow-up, stable position and full patency of stents with right-to-left or bidirectional shunt at atrial level and absence of syncope was confirmed in patients of both groups. Patients from the “intermediate” risk group demonstrated higher functional class, better performance of walking test, and lower levels of brain natriuretic peptide. Stenting atrioseptostomy reliably secured interatrial communication and improved clinical condition in patients with idiopathic pulmonary arterial hypertension. Mid-term results were better in “intermediate” risk group.
Tsallis Entropy for Assessing Spatial Uncertainty Associated with Mean Annual Runoff of Quaternary Catchments of the Middle Vaal Basin in South Africa
Authors: Ilunga Masengo
Published: September 2020

This study assesses mainly the uncertainty of the mean annual runoff (MAR) for quaternary catchments (QCs) considered as metastable nonextensive systems (from Tsalllis entropy) in the Middle Vaal catchment. The study is applied to the surface water resources (WR) of the South Africa 1990 (WR90), 2005 (WR2005) and 2012 (WR2012) data sets. The q-information index (from the Tsalllis entropy) is used here as a deviation indicator for the spatial evolution of uncertainty for the different QCs, using the Shannon entropy as a baseline. It enables the determination of a (virtual) convergence point, zone of positive and negative uncertainty deviation, zone of null deviation and chaotic zone for each data set. Such a determination is not possible on the basis of the Shannon entropy alone as a measure for the MAR uncertainty of QCs, i.e., when they are viewed as extensive systems. Finally, the spatial distributions for the zones of the q-uncertainty deviation (gain or loss in information) of the MAR are derived and lead to iso q-uncertainty deviation maps.
Takagi–Sugeno Observer Design for Remaining Useful Life Estimation of Li-Ion Battery System Under Faults
Authors: Kukurowski Norbert , Pazera Marcin , Witczak Marcin
Published: September 2020

Among the existing estimation schemes of a battery state of charge, most deal with an assumption that the faults will never occur in the system. Nevertheless, faults may have a crucial impact on the state of charge estimation accuracy. The paper proposes a novel observer design to estimate the state of charge and the remaining useful life of a Li-ion battery system under voltage and current measurement faults. The approach starts with converting the battery system into the descriptor Takagi–Sugeno form, where the state includes the original states along with the voltage and current measurement faults. Moreover, external disturbances are bounded by an ellipsoid based on the so-called Quadratic Boundedness approach, which ensures the system stability. The second-order Resistor-Capacitor equivalent circuit model is considered to verify the performance and correctness of the proposed observer. Subsequently, a real battery model is designed with experimental data of the Li-ion 18650 battery delivered from the NASA benchmark. Another experiment deals with an automated guided vehicle fed with a battery of which the remaining useful life is estimated. Finally, the results are compared with another estimation scheme based on the same benchmark.
Synchronous Condenser’s Loss of Excitation and Its Impact on the Performance of UHVDC
Authors: Guo Zhilin , Hao Liangliang , Wu Junyong , Wang Xingguo , Cao Hong , Wang Guang
Published: September 2020

The synchronous condenser (SC) has a broad application prospect in ultra-high-voltage direct current (UHVDC) systems. The SC’s loss of excitation (LOE) is an important grid-related fault that may cause damage to the UHVDC. However, as the premise of the scientific protection configuration, knowledge of the SC’s LOE feature and its impact on UHVDC is still missing. This article first analyzes the SC’s LOE feature, offering a basic cognition of this fault. Secondly, the LOE SC’s reactive power response to system voltage variation is studied in the single-machine infinite-bus system. This lends a foundation for transient UHVDC research. Finally, the LOE SC’s impacts on steady and transient UHVDC are evaluated, respectively, considering different AC strengths and system faults through PSCAD/EMTDC (V4.6, Manitoba HVDC Research Center, Winnipeg, MB, Canada) simulations. The results show that: (1) LOE the SC absorbs reactive power while maintaining synchronous operation, its excitation current declines monotonically; (2) the LOE SC has an insignificant effect on steady-state UHVDC; (3) the LOE SC can restrain the overvoltage and benefit the rectifier’s transient stability; and (4) to reduce the inverter’s commutation failure, keeping LOE SC is more effective than separating it beforehand, while separating the LOE SC after the system voltage drop performs best. These conclusions could provide insights for the protection’s criterion and operation mode selections.
Transitioning All Energy in 74 Metropolitan Areas, Including 30 Megacities, to 100% Clean and Renewable Wind, Water, and Sunlight (WWS)
Authors: Jacobson Mark Z. , von Krauland Anna-Katharina , Burton Zachary F.M. , Coughlin Stephen J. , Jaeggli Caitlin , Nelli Daniel , Nelson Alexander J. H. , Shu Yanbo , Smith Miles , Tan Chor , Wood Connery D. , Wood Kelyn D.
Published: September 2020

To date, roadmaps and policies for transitioning from fossil fuels to clean, renewable energy have been developed for nations, provinces, states, cities, and towns in order to address air pollution, global warming, and energy insecurity. However, neither roadmaps nor policies have been developed for large metropolitan areas (aggregations of towns and cities), including megacities (metropolitan areas with populations above 10 million). This study bridges that gap by developing roadmaps to transition 74 metropolitan areas worldwide, including 30 megacities, to 100% wind, water, and sunlight (WWS) energy and storage for all energy sectors by no later than 2050, with at least 80% by 2030. Among all metropolitan areas examined, the full transition may reduce 2050 annual energy costs by 61.1% (from $2.2 to $0.86 trillion/yr in 2013 USD) and social costs (energy plus air pollution plus climate costs) by 89.6% (from $8.3 to $0.86 trillion/yr). The large energy cost reduction is due to the 57.1% lower end-used energy requirements and the 9% lower cost per unit energy with WWS. The air pollution cost reduction of ~$2.6 (1.5–4.6) trillion/yr is due mostly to the saving of 408,000 (322,000–506,000) lives/yr with WWS. Global climate cost savings due to WWS are ~$3.5 (2.0–7.5) trillion/yr (2013 USD). The transition may also create ~1.4 million more long-term, full-time jobs than lost. Thus, moving to 100% clean, renewable energy and storage for all purposes in metropolitan areas can result in significant economic, health, climate, and job benefits.