bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: A Case of Unusual Combination of Hypothyroidism, Myocardial Bridging, and Myocardial Infarction-Induced Left Ventricular Aneurysm
Authors: Li Yueliang , Liu Zhengjiang
Published: November 2021

Background: Myocardial bridging (MB) of the coronary artery is a congenital anatomical variation, which has traditionally been considered a benign condition that does not cause cardiovascular events. However, recent studies have shown that MB is associated with major adverse cardiac events, including angina, myocardial infarction, arrhythmia, syncope, and even sudden death.

Case: We report a case of a 41-year-old man who had hypothyroidism and MB associated with ventricular aneurysm following myocardial infarction. This patient was admitted to our hospital because of 11 days of sudden discomfort and pain in the chest. An electrocardiogram on admission showed an old myocardial infarction. Coronary angiography showed MB in the distal segment of the left anterior descending artery. Left ventricular angiography, which was performed using a pigtail catheter, showed ventricular aneurysm formation. Thyroid ultrasound demonstrated hypothyroidism and Hashimoto's thyroiditis. Patients with hypothyroidism and MB have a high risk of acute myocardial infarction or even sudden death.

Conclusion: Observations in our case suggest that early recognition of hypothyroidism and MB is important for risk stratification and prognosis in patients with myocardial necrosis and acute coronary syndrome. Additionally, this early recognition may have positive effects on cardiovascular outcomes in patients with hypothyroidism.
Incidence of Arrhythmias and Their Prognostic Value in Patients With Multiple Myeloma
Authors: Li Yongxin , Tang Manyun , Zhong Liang , Wei Suhua , Song Jingzhuo , Liu Hui , Sun Chaofeng , Wang Jie
Published: November 2021

Background: Arrhythmias are common cardiovascular complications in multiple myeloma (MM) patients and are related to a poor prognosis.

Objective: This study aimed to assess the burden of arrhythmias and their prognostic value in patients with MM.

Methods: This was a retrospective study of patients with MM between January 2015 and April 2020 at the First Affiliated Hospital of Xi'an Jiaotong University. The incidence of arrhythmia and associated risk factors were evaluated. The relationship between the type of arrhythmia and survival was analyzed.

Results: A total of 319 patients with MM were identified, and 48.0% (153/319) had arrhythmias. The most common type of arrhythmia was sinus tachycardia (ST) (15.0%, 48/319), followed by sinus bradycardia (SB) (14.4%, 46/319), premature atrial contractions (PACs) (6.3%, 20/319), conduction disorders (CDs) (6.0%, 19/319), atrial fibrillation (AF) (6.0%, 19/319), premature ventricular contractions (PVCs) (4.4%, 14/319) and paroxysmal supraventricular tachycardia (PSVT) (0.6%, 2/319). The patients with arrhythmias had higher levels of log NT-proBNP and creatinine, greater bortezomib use, and a higher incidence of diabetes than those without arrhythmias (P < 0.05). The all-cause mortality rates of patients without arrhythmias and those with AF, ST, PACs, CDs, SB, and PVCs were 50.6% (84/166), 73.7% (14/19), 60.4% (29/48), 60.0% (12/20), 52.6% (10/19), 34.8% (16/46), and 28.6% (4/14), respectively. In a subgroup analysis of patients experiencing different types of arrhythmias, patients with SB had lower all-cause mortality than patients with AF (P < 0.01). Univariate and multivariate Cox analyses showed that there was a positive statistically significant association between SB and survival (HR: 0.592 [0.352–0.998], P = 0.049) in a subgroup analysis of different arrhythmias.

Conclusions: Patients with MM had a heavy arrhythmia burden, and in this study, approximately half of MM patients had arrhythmias. MM patients with SB were associated with lower all-cause mortality than those with AF. SB might be an independent positive factor for prognosis.
Lithofacies Types, Assemblage Characteristics, and Sedimentary Evolution Model of Lacustrine Shale in Dongyuemiao Formation of Fuxing Area
Authors: Shu Yi , Bao Hanyong , Zheng Youheng , Chen Miankun , Lu Yongchao , Liu Haotian , Peng Wei , Zhou Lin , Ma Yiquan , Wen Yaru , Wang Qiming , Zhang Zhiyao
Published: November 2021

The identification and classification of lithofacies’ types are very important activities in shale oil and gas exploration and development evaluation. There have been many studies on the classification of marine shale lithofacies, but research on lacustrine shale lithofacies is still in its infancy. Therefore, in this study, a high-resolution sequence stratigraphic framework is established for the lacustrine shale of the Jurassic Dongyuemiao Formation in the Fuxing area using detailed core observations, thin section identification, XRD analysis, major and trace element analysis, wavelet transform analysis, and detailed identification and characterization of the fossil shell layers in the formation. In addition, the lithofacies’ types and assemblages are identified and characterized, and the lithofacies’ characteristics and sedimentary evolution models in different sequence units are analyzed. The significance of the lithofacies assemblages for shale oil and gas exploration is also discussed. The results show that the shale of the target interval can be divided into 8 parasequence sets; further, 9 types of lithofacies and 6 types of lithofacies assemblages are identified. The 9 lithofacies are massive bioclast-containing limestone shoal facies (LF1), thick-layered fossil shell–containing limestone facies (LF2), layered mud-bearing fossil shell–containing limestone facies (LF3), laminated fossil shell–containing argillaceous shale facies (LF4), laminated fossil shell–bearing argillaceous shale facies (LF5), argillaceous shale facies (LF6), massive storm event–related bioclast-containing facies (LF7), massive argillaceous limestone facies (LF8), and massive mudstone facies (LF9). The sedimentary evolution models of different lithofacies are established as follows: Unit 1 (LF1-LF6) of the Dong-1 Member corresponds to the early stage of a lake transgressive system tract, and Units 2–4 (LF4-LF7) correspond to the middle to late stage of the lake transgressive system tract, which was an anoxic sedimentary environment. The Dong-2 Member (LF7-LF8) and the Dong-3 Member (LF5+LF9) correspond to a lake regressive system tract, which was an oxygen-rich sedimentary environment. Based on the characteristics of the shale lithofacies, sedimentary environment, and the quality of the reservoir, the lithofacies assemblage of LF4–LF7 in Unit 4 is the most favorable type for oil and gas exploration, followed by the lithofacies assemblage in Unit 2; the lithofacies assemblage in the Dong-2 and Dong-3 Members are the worst.
Treatment of Aggressive Pituitary Adenomas: A Case-Based Narrative Review
Authors: Cooper Odelia , Bonert Vivien , Liu Ning-Ai , Mamelak Adam N.
Published: November 2021

Management of aggressive pituitary adenomas is challenging due to a paucity of rigorous evidence supporting available treatment approaches. Recent guidelines emphasize the need to maximize standard therapies as well as the use of temozolomide and radiation therapy to treat disease recurrence. However, often these adenomas continue to progress over time, necessitating the use of additional targeted therapies which also impact quality of life and long-term outcomes. In this review, we present 9 cases of aggressive pituitary adenomas to illustrate the importance of a multidisciplinary, individualized approach. The timing and rationale for surgery, radiation therapy, temozolomide, somatostatin receptor ligands, and EGFR, VEGF, and mTOR inhibitors in each case are discussed within the context of evidence-based guidelines and clarify strategies for implementing an individualized approach in the management of these difficult-to-treat-adenomas.
Review and Perspectives of Machine Learning Methods for Wind Turbine Fault Diagnosis
Authors: Tang Mingzhu , Zhao Qi , Wu Huawei , Wang Ziming , Meng Caihua , Wang Yifan
Published: November 2021

Wind turbines (WTs) generally comprise several complex and interconnected systems, such as hub, converter, gearbox, generator, yaw system, pitch system, hydraulic system control system,integration control system, and auxiliary system. Moreover, fault diagnosis plays an important role in ensuring WT safety. In the past decades, machine learning (ML) has showed a powerful capability in fault detection and diagnosis of WTs, thereby remarkably reducing equipment downtime and minimizing financial losses. This study provides a comprehensive review of recent studies on ML methods and techniques for WT fault diagnosis. These studies are classified as supervised, unsupervised, and semi-supervised learning methods. Existing state-of-the-art methods are analyzed and characteristics are discussed. Perspectives on challenges and further directions are also provided.
Sequence Diversity, Locus Structure, and Evolutionary History of the SpTransformer Genes in the Sea Urchin Genome
Authors: Barela Hudgell Megan A. , Smith L. Courtney
Published: November 2021

The generation of large immune gene families is often driven by evolutionary pressure exerted on host genomes by their pathogens, which has been described as the immunological arms race. The SpTransformer (SpTrf) gene family from the California purple sea urchin, Strongylocentrotus purpuratus, is upregulated upon immune challenge and encodes the SpTrf proteins that interact with pathogens during an immune response. Native SpTrf proteins bind both bacteria and yeast, and augment phagocytosis of a marine Vibrio, while a recombinant SpTrf protein (rSpTrf-E1) binds a subset of pathogens and a range of pathogen associated molecular patterns. In the sequenced sea urchin genome, there are four SpTrf gene clusters for a total of 17 genes. Here, we report an in-depth analysis of these genes to understand the sequence complexities of this family, its genomic structure, and to derive a putative evolutionary history for the formation of the gene clusters. We report a detailed characterization of gene structure including the intron type and UTRs with conserved transcriptional start sites, the start codon and multiple stop codons, and locations of polyadenylation signals. Phylogenetic and percent mismatch analyses of the genes and the intergenic regions allowed us to predict the last common ancestral SpTrf gene and a theoretical evolutionary history of the gene family. The appearance of the gene clusters from the theoretical ancestral gene may have been driven by multiple duplication and deletion events of regions containing SpTrf genes. Duplications and ectopic insertion events, indels, and point mutations in the exons likely resulted in the extant genes and family structure. This theoretical evolutionary history is consistent with the involvement of these genes in the arms race in responses to pathogens and suggests that the diversification of these genes and their encoded proteins have been selected for based on the survival benefits of pathogen binding and host protection.
XAV-19, a Swine Glyco-Humanized Polyclonal Antibody Against SARS-CoV-2 Spike Receptor-Binding Domain, Targets Multiple Epitopes and Broadly Neutralizes Variants
Authors: Vanhove Bernard , Marot Stéphane , So Ray T. , Gaborit Benjamin , Evanno Gwénaëlle , Malet Isabelle , Lafrogne Guillaume , Mevel Edwige , Ciron Carine , Royer Pierre-Joseph , Lheriteau Elsa , Raffi François , Bruzzone Roberto , Mok Chris Ka Pun , Duvaux Odile , Marcelin Anne-Geneviève , Calvez Vincent
Published: November 2021

Amino acid substitutions and deletions in the Spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants can reduce the effectiveness of monoclonal antibodies (mAbs). In contrast, heterologous polyclonal antibodies raised against S protein, through the recognition of multiple target epitopes, have the potential to maintain neutralization capacities. XAV-19 is a swine glyco-humanized polyclonal neutralizing antibody raised against the receptor binding domain (RBD) of the Wuhan-Hu-1 Spike protein of SARS-CoV-2. XAV-19 target epitopes were found distributed all over the RBD and particularly cover the receptor binding motives (RBMs), in direct contact sites with the angiotensin converting enzyme-2 (ACE-2). Therefore, in Spike/ACE-2 interaction assays, XAV-19 showed potent neutralization capacities of the original Wuhan Spike and of the United Kingdom (Alpha/B.1.1.7) and South African (Beta/B.1.351) variants. These results were confirmed by cytopathogenic assays using Vero E6 and live virus variants including the Brazil (Gamma/P.1) and the Indian (Delta/B.1.617.2) variants. In a selective pressure study on Vero E6 cells conducted over 1 month, no mutation was associated with the addition of increasing doses of XAV-19. The potential to reduce viral load in lungs was confirmed in a human ACE-2 transduced mouse model. XAV-19 is currently evaluated in patients hospitalized for COVID-19-induced moderate pneumonia in phase 2a-2b (NCT04453384) where safety was already demonstrated and in an ongoing 2/3 trial (NCT04928430) to evaluate the efficacy and safety of XAV-19 in patients with moderate-to-severe COVID-19. Owing to its polyclonal nature and its glyco-humanization, XAV-19 may provide a novel safe and effective therapeutic tool to mitigate the severity of coronavirus disease 2019 (COVID-19) including the different variants of concern identified so far.
Peruvian Fur Seals as Archivists of El Niño Southern Oscillation Effects
Authors: Edwards Mickie R. , Cárdenas-Alayza Susana , Adkesson Michael J. , Daniels-Abdulahad Mya , Hirons Amy C.
Published: November 2021

Peru’s coastal waters are characterized by significant environmental fluctuation due to periodic El Niño- La Niña- Southern Oscillation (ENSO) events. This variability results in ecosystem-wide food web changes which are reflected in the tissues of the Peruvian fur seal (Arctocephalus australis). Stable isotope ratios (δ13C and δ15N) in Peruvian fur seal vibrissae (whiskers) are used to infer temporal primary production and dietary variations in individuals. Sea surface temperature anomaly (SSTA) recordings from the Niño 1+2 Index region captured corresponding ENSO conditions. Fluctuations in δ15N values were correlated to SSTA records, indicating that ENSO conditions likely impact the diet of these apex predators over time. Anomalous warm phase temperatures corresponded to decreased δ15N values, whereas cold phase anomalous conditions corresponded to increased δ15N values, potentially from upwelled, nutrient-rich water. Vibrissae δ13C values revealed general stability from 2004 to 2012, a moderate decline during 2013 (La Niña conditions) followed by a period of increased values concurrent with the 2014–2016 El Niño event. Both δ13C and δ15N values were inversely correlated to each other during the strongest El Niño Southern Oscillation event on record (2014–2016), possibly indicating a decline in production leading to an increase in food web complexity. Lower δ13C and δ15N values were exhibited in female compared to male fur seal vibrissae. Findings suggest ENSO conditions influence resource availability, possibly eliciting changes in pinniped foraging behavior as well as food web of the endangered Peruvian fur seal.
Community Development Quotas and Support of Small-Scale Fisheries as Two Key Concepts for Blue Growth in Fisheries
Authors: Sulanke Erik , Rybicki Sandra
Published: November 2021

Blue Growth has become one of the key topics of ocean management. It is defined as a holistic framework for an environmentally friendly and socio-economically sustainable development of ocean-related activities with a special emphasis on technological innovation. Capture fisheries are widely considered to have no substantial growth potential and consequently are not subject to the European Union’s Blue Growth strategy. In our review, we, however, argue that capture fisheries should play an essential role in national Blue Growth strategies. We identified two interconnected management strategies to foster Blue Growth in fisheries, a) the implementation of Community Development Quota (CDQ) systems and b) the support of small-scale fisheries (SSF). They hold the potential to benefit fishery-dependent coastal communities and therefore counteract consolidations in the fishing sector. Additionally, they provide the possibility to improve quota access for small-scale fishermen. Besides having better access to quota, the future of SSF depends on sources of public funding for technical improvement and innovation as well as increased representation in the management. In this perspective, we present different cases that successfully implemented CDQs (the Alaska pollock fishery) or have considerable potential to implement CDQ programs or improve their current approaches (United Kingdom, Ireland, and Iceland). We further discuss examples for successful management strategies to support SSF directly. If these aspects are considered in a Blue Growth strategy, the survival of fishery-dependent communities could be assured, and SSF could develop from predominantly part-time or subsistence fisheries to a full-time occupation. By those means, they would be part of a fostering Blue Economy and strengthen environmentally friendly and socio-economically sustainable fishing practices in Europe.
Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea
Authors: Li Xiaopeng , Dai Zehan , Di Pengfei , Feng Junxi , Tao Jun , Chen Duofu , Li Niu , Li Yazi
Published: November 2021

Methane seeps are chemosynthetic ecosystems in the deep-sea environment. Microbial community structures have been extensively studied in the seepage-affected sediments and investigation in the water column above the seeping sites is still lacking. In this study, prokaryotic communities in the bottom water about 50 cm from the seabed at methane seeps with various seepage intensities in Haima, South China Sea were comparatively studied by using 16S ribosomal RNA gene sequencing. These sites were assigned based on their distinct methane content levels and seafloor landscapes as the non-seepage (NS) site, low-intensity seepage (LIS) site, and high-intensity seepage (HIS) site. The abundances of the dominant phyla Proteobacteria, Bacteroidetes, and Actinobacteria differed significantly between NS and the two seepage sites (p < 0.05). Alpha diversity differed among the three sites with the HIS site showing the lowest community diversity. Principal component analysis revealed highly divergent bacterial community structures at three sites. Many environmental variables including temperature, alkalinity, pH, methane, dissolved organic carbon (DOC), and inorganic nutrients were measured. Redundancy analysis indicated that methane content is the key environmental factor driving bacterial community variation (p = 0.001). Linear discriminant analysis effect size analysis identified various differentially enriched genera at the LIS and HIS sites. Phylogenetic analysis revealed close phylogenetic relationship among the operational taxonomic units of these genera with known oil-degrading species, indicating oil seepage may occur at the Haima cold seeps. Co-occurrence networks indicated that the strength of microbial interactions was weakest at the HIS site. This study represents a comprehensive comparison of microbial profiles in the water column of cold seeps in the SCS, revealing that the seepage intensity has a strong impact on bacterial community dynamics.
Hurricane Flood Hazard Assessment for the Archipelago of San Andres, Providencia and Santa Catalina, Colombia
Authors: Rey Wilmer , Ruiz-Salcines Pablo , Salles Paulo , Urbano-Latorre Claudia P. , Escobar-Olaya Germán , Osorio Andrés F. , Ramírez Juan Pablo , Cabarcas-Mier Angélica , Jigena-Antelo Bismarck , Appendini Christian M.
Published: November 2021

Despite the low occurrence of tropical cyclones at the archipelago of San Andres, Providencia, and Santa Catalina (Colombia), Hurricane Iota in 2020 made evident the area vulnerability to tropical cyclones as major hazards by obliterating 56.4 % of housing, partially destroying the remaining houses in Providencia. We investigated the hurricane storm surge inundation in the archipelago by forcing hydrodynamic models with synthetic tropical cyclones and hypothetical hurricanes. The storm surge from synthetic events allowed identifying the strongest surges using the probability distribution, enabling the generation of hurricane storm surge flood maps for 100 and 500 year return periods. This analysis suggested that the east of San Andres and Providencia are the more likely areas to be flooded from hurricanes storm surges. The hypothetical events were used to force the hydrodynamic model to create worst-case flood scenario maps, useful for contingency and development planning. Additionally, Hurricane Iota flood levels were calculated using 2D and 1D models. The 2D model included storm surge (SS), SS with astronomical tides (AT), and SS with AT and wave setup (WS), resulting in a total flooded area (percentage related to Providencia’s total area) of 67.05 ha (3.25 %), 65.23 ha (3.16 %), and 76.68 ha (3.68%), respectively. While Hurricane Iota occurred during low tide, the WS contributed 14.93 % (11.45 ha) of the total flooded area in Providencia. The 1D approximation showed that during the storm peak in the eastern of the island, the contribution of AT, SS, and wave runup to the maximum sea water level was −3.01%, 46.36%, and 56.55 %, respectively. This finding provides evidence of the water level underestimation in insular environments when modeling SS without wave contributions. The maximum SS derived from Iota was 1.25 m at the east of Providencia, which according to this study has an associated return period of 3,234 years. The methodology proposed in this study can be applied to other coastal zones and may include the effect of climate change on hurricane storm surges and sea-level rise. Results from this study are useful for emergency managers, government, coastal communities, and policymakers as civil protection measures.
Pathophysiology of Coagulopathy Induced by Traumatic Brain Injury Is Identical to That of Disseminated Intravascular Coagulation With Hyperfibrinolysis
Authors: Wada Takeshi , Shiraishi Atsushi , Gando Satoshi , Yamakawa Kazuma , Fujishima Seitaro , Saitoh Daizoh , Kushimoto Shigeki , Ogura Hiroshi , Abe Toshikazu , Mayumi Toshihiko , Sasaki Junichi , Kotani Joji , Takeyama Naoshi , Tsuruta Ryosuke , Takuma Kiyotsugu , Shiraishi Shin-ichiro , Shiino Yasukazu , Nakada Taka-aki , Okamoto Kohji , Sakamoto Yuichiro , Hagiwara Akiyoshi , Fujimi Satoshi , Umemura Yutaka , Otomo Yasuhiro
Published: November 2021

Background: Traumatic brain injury (TBI)-associated coagulopathy is a widely recognized risk factor for secondary brain damage and contributes to poor clinical outcomes. Various theories, including disseminated intravascular coagulation (DIC), have been proposed regarding its pathomechanisms; no consensus has been reached thus far. This study aimed to elucidate the pathophysiology of TBI-induced coagulopathy by comparing coagulofibrinolytic changes in isolated TBI (iTBI) to those in non-TBI, to determine the associated factors, and identify the clinical significance of DIC diagnosis in patients with iTBI.

Methods: This secondary multicenter, prospective study assessed patients with severe trauma. iTBI was defined as Abbreviated Injury Scale (AIS) scores ≥4 in the head and neck, and ≤2 in other body parts. Non-TBI was defined as AIS scores ≥4 in single body parts other than the head and neck, and the absence of AIS scores ≥3 in any other trauma-affected parts. Specific biomarkers for thrombin and plasmin generation, anticoagulation, and fibrinolysis inhibition were measured at the presentation to the emergency department (0 h) and 3 h after arrival.

Results: We analyzed 34 iTBI and 40 non-TBI patients. Baseline characteristics, transfusion requirements and in-hospital mortality did not significantly differ between groups. The changes in coagulation/fibrinolysis-related biomarkers were similar. Lactate levels in the iTBI group positively correlated with DIC scores (rho = −0.441, p = 0.017), but not with blood pressure (rho = −0.098, p = 0.614). Multiple logistic regression analyses revealed that the injury severity score was an independent predictor of DIC development in patients with iTBI (odds ratio = 1.237, p = 0.018). Patients with iTBI were further subdivided into two groups: DIC (n = 15) and non-DIC (n = 19) groups. Marked thrombin and plasmin generation were observed in all patients with iTBI, especially those with DIC. Patients with iTBI and DIC had higher requirements for massive transfusion and emergency surgery, and higher in-hospital mortality than those without DIC. Furthermore, DIC development significantly correlated with poor hospital survival; DIC scores at 0 h were predictive of in-hospital mortality.

Conclusions: Coagulofibrinolytic changes in iTBI and non-TBI patients were identical, and consistent with the pathophysiology of DIC. DIC diagnosis in the early phase of TBI is key in predicting the outcomes of severe TBI.
Factors Associated With Clinical and Radiographic Severity in People With Osteoarthritis: A Cross-Sectional Population-Based Study
Authors: Costa Daniela , Cruz Eduardo B. , Silva Catarina , Canhão Helena , Branco Jaime , Nunes Carla , Rodrigues Ana M.
Published: November 2021

Background: Hip/knee osteoarthritis (HKOA) is a leading cause of disability and imposes a major socioeconomic burden. The aim of this study is to estimate the prevalence of HKOA in Portugal, characterised the clinical severity of HKOA in the population, and identified sociodemographic, lifestyle, and clinical factors associated with higher clinical and radiographic severity.

Methods: Participants with a diagnosis of HKOA from the EpiReumaPt study (2011–2013) were included (n = 1,087). Hip/knee osteoarthritis diagnosis was made through a structured evaluation by rheumatologists according to American College of Rheumatology criteria. Clinical severity was classified based on Hip Disability and Osteoarthritis Outcome Scale (HOOS) and Knee Injury and Osteoarthritis Outcome Scale (KOOS) score tertiles. Radiographic severity was classified based on the Kellgren-Lawrence grades as mild, moderate, or severe. Sociodemographic lifestyle and clinical variables, including the presence of anxiety and depression symptoms, were analysed. Factors associated with higher clinical and radiographic severity were identified using ordinal logistic regression models.

Results: Hip/knee osteoarthritis diagnosis was present in 14.1% of the Portuguese population [12.4% with knee osteoarthritis (OA) and 2.9% with hip OA]. Clinical severity was similar between people with hip (HOOS = 55.79 ± 20.88) and knee (KOOS = 55.33 ± 20.641) OA. People in the high HOOS/KOOS tertile tended to be older (64.39 ± 0.70 years), female (75.2%), overweight (39.0%) or obese (45.9%), and had multimorbidity (86.1%). Factors significantly associated with higher clinical severity tertile were age [55–64 years: odds ratio (OR) = 3.18; 65–74 years: OR = 3.25; ≥75 years: OR = 4.24], female sex (OR = 1.60), multimorbidity (OR = 1.75), being overweight (OR = 2.01) or obese (OR = 2.82), and having anxiety symptoms (OR = 1.83). Years of education was inversely associated with higher clinical severity. Factors significantly associated with higher radiographic severity were age (65–74 years: OR = 3.59; ≥75 years: OR = 3.05) and being in the high HOOS/KOOS tertile (OR = 4.91). Being a female and live in Lisbon or in the Centre region were inversely associated with the higher radiographic severity.

Conclusion: Hip/knee osteoarthritis is present in ~1.1 million of Portuguese people. Age, educational level, and obesity are independently associated with HKOA clinical severity, whereas age, sex, geographic location, and clinical severity are independently associated with radiographic severity.
The Novel Agrotis ipsilon Nora Virus Confers Deleterious Effects to the Fitness of Spodoptera frugiperda (Lepidoptera: Noctuidae)
Authors: Li Tong , Guan Ruobing , Wu Yuqing , Chen Su , Yuan Guohui , Miao Xuexia , Li Haichao
Published: November 2021

In the present study, we identified a novel, positive-sense single-stranded RNA virus in the Chinese black cutworm, Agrotis ipsilon. It has a genome length of 11,312 nucleotides, excluding the poly(A) tails, and contains five open reading frames. The ORF2 encodes the conserved domains of RNA helicase and RNA-dependent RNA polymerase, while ORF4 and 5 encode three viral proteins. Herein, the A. ipsilon virus was clustered with a Helicoverpa armigera Nora virus and was thus provisionally named “Agrotis ipsilon Nora virus” (AINV). AINV was successfully transmitted into a novel host, Spodoptera frugiperda, through injection, causing a stable infection. This found the possibility of horizontal AINV transmission among moths belonging to the same taxonomic family. Nonetheless, AINV infection was deleterious to S. frugiperda and mainly mediated by antiviral and amino acid metabolism-related pathways. Furthermore, the infection significantly increased the S. frugiperda larval period but significantly reduced its moth eclosion rate. It suggests that AINV is probably to be a parasitic virus of S. frugiperda.
Lactobacillus plantarum Lipoteichoic Acids Possess Strain-Specific Regulatory Effects on the Biofilm Formation of Dental Pathogenic Bacteria
Authors: Lee Dongwook , Im Jintaek , Park Dong Hyun , Jeong Sungho , Park Miri , Yoon Seokmin , Park Jaewoong , Han Seung Hyun
Published: November 2021

Bacterial biofilm residing in the oral cavity is closely related to the initiation and persistence of various dental diseases. Previously, we reported the anti-biofilm activity of Lactobacillus plantarum lipoteichoic acid (Lp.LTA) on a representative dental cariogenic pathogen, Streptococcus mutans. Since LTA structure varies in a bacterial strain-specific manner, LTAs from various L. plantarum strains may have differential anti-biofilm activity due to their distinct molecular structures. In the present study, we isolated Lp.LTAs from four different strains of L. plantarum (LRCC 5193, 5194, 5195, and 5310) and compared their anti-biofilm effects on the dental pathogens, including S. mutans, Enterococcus faecalis, and Streptococcus gordonii. All Lp.LTAs similarly inhibited E. faecalis biofilm formation in a dose-dependent manner. However, their effects on S. gordonii and S. mutans biofilm formation were different: LRCC 5310 Lp.LTA most effectively suppressed the biofilm formation of all strains of dental pathogens, while Lp.LTAs from LRCC 5193 and 5194 hardly inhibited or even enhanced the biofilm formation. Furthermore, LRCC 5310 Lp.LTA dramatically reduced the biofilm formation of the dental pathogens on the human dentin slice infection model. Collectively, these results suggest that Lp.LTAs have strain-specific regulatory effects on biofilm formation of dental pathogens and LRCC 5310 Lp.LTA can be used as an effective anti-biofilm agent for the prevention of dental infectious diseases.
Functionalized Nanoparticles in Prevention and Targeted Therapy of Viral Diseases With Neurotropism Properties, Special Insight on COVID-19
Authors: Ren Meishen , Wang Yin , Luo Yan , Yao Xueping , Yang Zexiao , Zhang Pengfei , Zhao Wei , Jiang Dike
Published: November 2021

Neurotropic viruses have neural-invasive and neurovirulent properties to damage the central nervous system (CNS), leading to humans’ fatal symptoms. Neurotropic viruses comprise a lot of viruses, such as Zika virus (ZIKV), herpes simplex virus (HSV), rabies virus (RABV), and severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Effective therapy is needed to prevent infection by these viruses in vivo and in vitro. However, the blood-brain barrier (BBB) usually prevents macromolecules from entering the CNS, which challenges the usage of the traditional probes, antiviral drugs, or neutralizing antibodies in the CNS. Functionalized nanoparticles (NPs) have been increasingly reported in the targeted therapy of neurotropic viruses due to their sensitivity and targeting characteristics. Therefore, the present review outlines efficient functionalized NPs to further understand the recent trends, challenges, and prospects of these materials.
Multi-Modal Image Fusion Based on Matrix Product State of Tensor
Authors: Lu Yixiang , Wang Rui , Gao Qingwei , Sun Dong , Zhu De
Published: November 2021

Multi-modal image fusion integrates different images of the same scene collected by different sensors into one image, making the fused image recognizable by the computer and perceived by human vision easily. The traditional tensor decomposition is an approximate decomposition method and has been applied to image fusion. In this way, the image details may be lost in the process of fusion image reconstruction. To preserve the fine information of the images, an image fusion method based on tensor matrix product decomposition is proposed to fuse multi-modal images in this article. First, each source image is initialized into a separate third-order tensor. Then, the tensor is decomposed into a matrix product form by using singular value decomposition (SVD), and the Sigmoid function is used to fuse the features extracted in the decomposition process. Finally, the fused image is reconstructed by multiplying all the fused tensor components. Since the algorithm is based on a series of singular value decomposition, a stable closed solution can be obtained and the calculation is also simple. The experimental results show that the fusion image quality obtained by this algorithm is superior to other algorithms in both objective evaluation metrics and subjective evaluation.
Case Report: Isolated Idiopathic Saccular Dysfunction
Authors: Waissbluth Sofia , Oyarzún Javier
Published: November 2021

Advances in vestibular testing have now allowed us to test each semicircular canal as well as the utricle and saccule, independently. This has led to the discovery of new patterns of vestibular dysfunction that were once impossible to evaluate. This report describes the case of a 20-year-old woman with a 2-month history of recurrent dizziness. She had a complete audiovestibular assessment. The only abnormality observed was the absence of a cervical vestibular-evoked myogenic potential response for the right side, hence an isolated saccular dysfunction. In conclusion, isolated otolithic dysfunction is probably an overlooked and neglected clinical presentation. Its true incidence is unknown, and further research is needed to understand this clinical entity.
Intraventricular Craniopharyngiomas—Overcoming Their Relative Inaccessibility: Institutional Experience With a Review of Literature
Authors: Deopujari Chandrashekhar , Behari Sanjay , Shroff Krishna , Kumar Ashutosh , Thombre Bhushan , Karmarkar Vikram , Mohanty Chandan
Published: November 2021

Introduction: Craniopharyngiomas constitute 2–4% of intracranial neoplasms. Intraventricular craniopharyngiomas (IVCrs) are the rarely encountered varieties of these lesions.

Objective: The objective of the study was to study the special features in clinical presentation, imaging, management, and surgical outcome of IVCrs.

Materials and Methods: This retrospective analysis included the combined experience from two tertiary care institutions. Medical records of histopathologically proven cases of IVCrs from January 1994 to June 2021 were assessed, and images were analyzed based on the criteria by Migliore et al. for inclusion of solely intraventricular lesion with the third ventricular ependyma demarcating it from the suprasellar cistern.

Results: Among the 25 patients included (mean age: 35.4 years), the most common presentation included headache (n = 21, 84%), vomiting and other features of raised ICP (n = 18, 72%), visual complaints (n = 12, 48%), and endocrinopathies (n = 11, 44%). Fifteen had predominantly cystic tumors, two were purely solid, and eight were of mixed consistency. Primary open microsurgical procedures were performed in 18 (72%) patients, of which four (16%) were endoscope-assisted. Seven (28%) underwent a purely endoscopic procedure. One underwent a staged surgery with endoscopic cyst fenestration and intracystic interferon (IFN)-alpha therapy, followed by microsurgical excision. Complete excision was achieved in 10 patients, near-total in nine, and partial excision in six. Four patients underwent a ventriculoperitoneal shunt (one before the definitive procedure). At a median follow-up of 36 months (range:11–147 months), five patients developed a recurrence, and one had a stable small residue. This patient and two others with small cystic recurrences were observed. One patient was managed with radiotherapy alone. Another underwent re-surgery after a trial of radiotherapy, and the last patient developed a local recurrence, which was managed with radiotherapy; he then later developed an intraparenchymal recurrence, which was operated.

Conclusion: Purely IVCrs present with raised intracranial pressure, and visual disturbances are less common. Their deep-seated location and limited surgical field-of-view makes minimally invasive endoscopic-assisted surgery most suitable for their excision. The thin-walled cystic lesions may be occasionally adherent to the ependymal wall in close vicinity to the thalamus–hypothalamus complex, making complete excision difficult. Their responsiveness to radiotherapy, often leads to a gratifying long-term outcome.
Association of Natriuretic Peptide With Adverse Outcomes and Disease Severity After Intracerebral Hemorrhage: A Systematic Review
Authors: Wang Jiahui , Wang Jingxuan , Tang Zhouping , Zhang Ping
Published: November 2021

Background: Over the past decade, many studies have reported the association of brain natriuretic peptide (BNP) and N-terminal pro-brain natriuretic peptide (NT-proBNP) with clinical outcome of intracerebral hemorrhage (ICH). However, a broad consensus has not been reached.

Objective: To evaluate the role of BNP/NT-proBNP levels in prognosis and disease severity assessment in patients with ICH.

Methods: A systematic literature search was conducted utilizing PubMed, Embase, Web of Science and the Cochrane Library databases up to July 23, 2021. Studies that explored the association between BNP/NT-proBNP level and clinical outcome or disease severity in ICH patients were eligible. Outcome measures were all-cause mortality, poor functional outcome, adverse cardiac events and markers of disease severity.

Results: Ten studies, involving 1,373 patients with ICH, met the inclusion criteria. Nine studies focused on clinical outcomes (five all-cause mortality, five functional outcomes, and one adverse cardiac event) and seven on disease severity. In terms of prognosis, all five studies showed an association between elevated BNP/NT-proBNP level and increased risk of all-cause mortality in ICH patients. Four of the five studies reported poor functional outcomes in patients with higher BNP/NT-proBNP levels and one study associated higher BNP/NT-proBNP levels with increased risk of adverse cardiac events. Moreover, two studies identified an additional predictive ability of BNP/NT-proBNP level beyond that of pre-existing prognostic variables. In terms of disease severity, five studies (71%) reported that BNP/NT-proBNP level correlated positively with hematoma volume in addition to ICH and GCS scores.

Conclusion: Elevated BNP/NT-proBNP level is associated with increased risk of all-cause mortality, poor functional outcome, adverse cardiac events and disease severity in patients with ICH. Thus, BNP/NT-proBNP level is a promising prognostic indicator for ICH and also an effective marker of disease severity. Current evidence remains limited by the small number and high heterogeneity of included studies. Further appropriately designed, large-scale studies are required to confirm the current findings.
Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process
Authors: Suárez-Montenegro Zully Jimena , Ballesteros-Vivas Diego , Gallego Rocío , Valdés Alberto , Sánchez-Martínez Jose David , Parada-Alfonso Fabián , Ibáñez Elena , Cifuentes Alejandro
Published: November 2021

Tamarillo (Cyphomandra betacea (Cav.) Sendt.), or tree tomato, is a tropical fruit from the Andean region of South America; it is highly rich in vitamins, minerals, and polyphenolic compounds. In this study, extracts from tamarillo epicarp (TE) were obtained by pressurized liquid extraction (PLE), and their in-vitro neuroprotective potential was assessed. A central composite design with response surface methodology was performed to optimize PLE as a function of solvent composition and temperature. Selected response variables were extraction yield, total phenolic content (TPC), total flavonoid content (TFC), total carotenoid content (TCC), antioxidant (ABTS), and anti-inflammatory (LOX) activities, and anti-acetylcholinesterase (AChE) inhibitory capacity. According to the desirability function, the optimal conditions were 100% ethanol and 180°C with a 0.87 desirability value. Next, the anti-butyrylcholinesterase enzyme (BChE), reactive oxygen species (ROS), and reactive nitrogen species (RNS) inhibition as well as cytotoxicity in HK-2, THP-1 monocytes, and SH-5YSY neuroblastoma cell lines were studied for the TE extract obtained under optimized conditions. The optimum TE extract provided the following results: extraction yield (36.25%), TPC (92.09 mg GAE/g extract), TFC (4.4 mg QE/g extract), TCC (107.15 mg CE/g extract), antioxidant capacity (ABTS, IC50 = 6.33 mg/ml extract), LOX (IC50 = 48.3 mg/ml extract), and AChE (IC50 = 97.46 mg/ml extract), and showed no toxicity at concentration up to 120 μg/ml extract for all the tested cell lines. Finally, chemical characterization by liquid chromatography-tandem mass spectrometry (UHPLC-q-TOF-MS/MS) of the optimum TE extract exhibited an important presence of hydroxycinnamic acid derivatives and other phenolic acids as well as quercetin hexoside and rutin, as main metabolites responsible for the observed biological properties. All these results suggested that TE, which represents between 8 and 15% of the total fruit, could become a promising natural by-product with a potential “multitarget” activity against Alzheimer's disease.
Deep Learning Plus Three-Dimensional Printing in the Management of Giant (>15 cm) Sporadic Renal Angiomyolipoma: An Initial Report
Authors: Gao Yunliang , Tang Yuanyuan , Ren Da , Cheng Shunhua , Wang Yinhuai , Yi Lu , Peng Shuang
Published: November 2021

Objective

To evaluate the feasibility and effectivity of deep learning (DL) plus three-dimensional (3D) printing in the management of giant sporadic renal angiomyolipoma (RAML).

Methods

The medical records of patients with giant (>15 cm) RAML were retrospectively reviewed from January 2011 to December 2020. 3D visualized and printed kidney models were performed by DL algorithms and 3D printing technology, respectively. Patient demographics and intra- and postoperative outcomes were compared between those with 3D-assisted surgery (3D group) or routine ones (control group).

Results

Among 372 sporadic RAML patients, 31 with giant ones were eligible for analysis. The median age was 40.6 (18–70) years old, and the median tumor size was 18.2 (15–28) cm. Seventeen of 31 (54.8%) had a surgical kidney removal. Overall, 11 underwent 3D-assisted surgeries and 20 underwent routine ones. A significant higher success rate of partial nephrectomy (PN) was noted in the 3D group (72.7% vs. 30.0%). Patients in the 3D group presented a lower reduction in renal function but experienced a longer operation time, a greater estimated blood loss, and a higher postoperative morbidity. Subgroup analysis was conducted between patients undergoing PN with or without 3D assistance. Despite no significant difference, patients with 3D-assisted PN had a slightly larger tumor size and higher nephrectomy score, possibly contributing to a relatively higher rate of complications. However, 3D-assisted PN lead to a shorter warm ischemia time and a lower renal function loss without significant difference. Another subgroup analysis between patients under 3D-assisted PN or 3D-assisted RN showed no statistically significant difference. However, the nearness of tumor to the second branch of renal artery was relatively shorter in 3D-assisted PN subgroup than that in 3D-assisted RN subgroup, and the difference between them was close to significant.

Conclusions

3D visualized and printed kidney models appear to be additional tools to assist operational management and avoid a high rate of kidney removal for giant sporadic RAMLs.
The Potential Prognostic Role of Oligosaccharide-Binding Fold-Containing Protein 2A (OBFC2A) in Triple-Negative Breast Cancer
Authors: Wu Qianxue , Tang Xin , Zhu Wenming , Li Qing , Zhang Xiang , Li Hongyuan
Published: November 2021

Background

Patients with triple-negative breast cancer (TNBC) have poor overall survival. The present study aimed to investigate the potential prognostics of TNBC by analyzing breast cancer proteomic and transcriptomic datasets.

Methods

Candidate proteins selected from CPTAC (the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium) were validated using datasets from METABRIC (Molecular Taxonomy of Breast Cancer International Consortium). Kaplan-Meier analysis and ROC (receiver operating characteristic) curve analysis were performed to explore the prognosis of candidate genes. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis were performed on the suspected candidate genes. Single-cell RNA-seq (scRNA-seq) data from GSE118389 were used to analyze the cell clusters in which OBFC2A (Oligosaccharide-Binding Fold-Containing Protein 2A) was mainly distributed. TIMER (Tumor Immune Estimation Resource) was used to verify the correlation between OBFC2A expression and immune infiltration. Clone formation assays and wound healing assays were used to detect the role of OBFC2A expression on the proliferation, invasion, and migration of breast cancer cells. Flow cytometry was used to analyze the effects of silencing OBFC2A on breast cancer cell cycle and apoptosis.

Results

Six candidate proteins were found to be differentially expressed in non-TNBC and TNBC groups from CPTAC. However, only OBFC2A was identified as an independently poor prognostic gene marker in METABRIC (HR=3.658, 1.881-7.114). And OBFC2A was associated with immune functions in breast cancer. Biological functional experiments showed that OBFC2A might promote the proliferation and migration of breast cancer cells. The inhibition of OBFC2A expression blocked the cell cycle in G1 phase and inhibited the transformation from G1 phase to S phase. Finally, downregulation of OBFC2A also increased the total apoptosis rate of cells.

Conclusion

On this basis, OBFC2A may be a potential prognostic biomarker for TNBC.