bluuebunny/crossref_metadata_2025 huggingface dataset:
Introduction to the Special Issue: New Frontiers in Acrylamide Study in Foods—Formation, Analysis and Exposure Assessment
Authors: Delgado-Andrade Cristina , Mesías Marta , Morales Francisco J.
Published: October 2020

Acrylamide is a chemical contaminant that naturally originates during the thermal processing of many foods. Since 2002, worldwide institutions with competencies in food safety have promoted activities aimed at updating knowledge for a revaluation of the risk assessment of this process contaminant. The European Food Safety Authority (EFSA) ruled in 2015 that the presence of acrylamide in foods increases the risk of developing cancer in any age group of the population. Commission Regulation (EU) 2017/2158 establishes recommended mitigation measures for the food industry and reference levels to reduce the presence of acrylamide in foods and, consequently, its harmful effects on the population. This Special Issue explores recent advances on acrylamide in foods, including a novel insight on its chemistry of formation and elimination, effective mitigation strategies, conventional and innovative monitoring techniques, risk/benefit approaches and exposure assessment, in order to enhance our understanding for this process contaminant and its dietary exposure.
Special Issue: Rheology and Quality Research of Cereal-Based Food
Authors: Raymundo Anabela , Torres María Dolores , Sousa Isabel
Published: October 2020

New trends in the cereal industry deal with a permanent need to develop new food products that are adjusted to consumer demands and, in the near future, the scarcity of food resources. Sustainable food products as health and wellness promoters can be developed redesigning traditional staple foods, using environmentally friendly ingredients (such as microalgae biomass or pulses) or by-products (e.g., tomato seeds) in accordance with the bioeconomy principles. These are topics that act as driving forces for innovation and will be discussed in the present special issue. Rheology always was the reference discipline to determine dough and bread properties. A routine analysis of cereal grains includes empirical rheology techniques that imply the use of well-known equipment in cereal industries (e.g., alveograph, mixograph, extensograph). Their parameters determine the blending of the grains and are crucial on the technical sheets that determine the use of flours. In addition, the structure of gluten-free cereal-based foods has proven to be a determinant for the appeal and strongly impacts consumers’ acceptance. Fundamental rheology has a relevant contribution to help overcome the technological challenges of working with gluten-free flours. These aspects will also be pointed out in order to provide a prospective view of the relevant developments to take place in the area of cereal technology.
A Nanoengineered Stainless Steel Surface to Combat Bacterial Attachment and Biofilm Formation
Authors: Ban Ga-Hee , Li Yong , Wall Marisa M. , Jun Soojin
Published: October 2020

Nanopatterning and anti-biofilm characterization of self-cleanable surfaces on stainless steel substrates were demonstrated in the current study. Electrochemical etching in diluted aqua regia solution consisting of 3.6% hydrogen chloride and 1.2% nitric acid was conducted at 10 V for 5, 10, and 15 min to fabricate nanoporous structures on the stainless steel. Variations in the etching rates and surface morphologic characteristics were caused by differences in treatment durations; the specimens treated at 10 V for 10 min showed that the nanoscale pores are needed to enhance the self-cleanability. Under static and realistic flow environments, the populations of Escherichia coli O157:H7 and Salmonella Typhimurium on the developed features were significantly reduced by 2.1–3.0 log colony-forming unit (CFU)/cm2 as compared to bare stainless steel (p < 0.05). The successful fabrication of electrochemically etched stainless steel surfaces with Teflon coating could be useful in the food industry and biomedical fields to hinder biofilm formation in order to improve food safety.
Foodborne Viruses and Innovative Non-Thermal Food-Processing Technologies
Authors: Pexara Andreana , Govaris Alexander
Published: October 2020

In recent years, several foodborne viruses’ outbreaks have been recorded worldwide. Μost of the foodborne viruses have a low infection dose, are stable and can persist and survive in foods for a long time without loss of infectivity. The most important foodborne viruses are: human norovirus (HuNoV), human rotavirus (HRV), hepatitis A virus (HAV), hepatitis E virus (HEV), human astrovirus (HAstV), Aichi virus (AiV), sapovirus (SaV), human adenovirus (HAdV) and enterovirus (EV). In recent years, innovative non-thermal food-processing technologies including high-pressure processing (HPP), cold plasma (CP), ultraviolet light (UV), irradiation and pulsed electric field (PEF) for improving the quality and safety of foods, including foods of animal origin, have been under research. This review presents the recent data on foodborne viruses and reviews the innovative non-thermal technologies for the control of the foodborne viruses in foods.
Human Capital Accumulation and the Evolution of Overconfidence
Authors: Santos-Pinto Luis
Published: October 2020

This paper studies the evolution of overconfidence over a cohort’s working life. To do this, the paper incorporates subjective assessments into a continuous time human capital accumulation model with a finite horizon. The main finding is that the processes of human capital accumulation, skill depreciation, and subjective assessments imply that overconfidence first increases and then decreases over the cohort’s working life. In the absence of skill depreciation, overconfidence monotonically increases over the cohort’s working life. The model generates four additional testable predictions. First, everything else equal, overconfidence peaks earlier in activities where skill depreciation is higher. Second, overconfidence is lower in activities where the distribution of income is more dispersed. Third, for a minority of individuals, overconfidence decreases over their working life. Fourth, overconfidence is lower with a higher market discount rate. The paper provides two applications of the model. It shows the model can help make sense of field data on overconfidence, experience, and trading activity in financial markets. The model can also explain experimental data on the evolution of overconfidence among poker and chess players.
Equiaffine Braneworld
Authors: Zhang Fan
Published: October 2020

Higher dimensional theories, wherein our four dimensional universe is immersed into a bulk ambient, have received much attention recently, and the directions of investigation had, as far as we can discern, all followed the ordinary Euclidean hypersurface theory’s isometric immersion recipe, with the spacetime metric being induced by an ambient parent. We note, in this paper, that the indefinite signature of the Lorentzian metric perhaps hints at the lesser known equiaffine hypersurface theory as being a possibly more natural, i.e., less customized beyond minimal mathematical formalism, description of our universe’s extrinsic geometry. In this alternative, the ambient is deprived of a metric, and the spacetime metric becomes conformal to the second fundamental form of the ordinary theory, therefore is automatically indefinite for hyperbolic shapes. Herein, we advocate investigations in this direction by identifying some potential physical benefits to enlisting the help of equiaffine differential geometry. In particular, we show that a geometric origin for dark energy can be proposed within this framework.
Identification of a Novel de Novo Variant in the SYT2 Gene Causing a Rare Type of Distal Hereditary Motor Neuropathy
Authors: Mironovich Olga , Dadali Elena , Malmberg Sergey , Markova Tatyana , Ryzhkova Oxana , Poliakov Aleksander
Published: October 2020

Objective: To report the first de novo missense mutation in the SYT2 gene causing distal hereditary motor neuropathy. Methods: Genetic testing was carried out, including clinical exome sequencing for the proband and Sanger sequencing for the proband and his parents. We described the clinical and electrophysiological features found in the patient. Results: We reported a proband with a new de novo missense mutation, c.917C>T (p.Ser306Leu), in the C2B domain of SYT2. The clinical presentation was similar to that of phenotypes described in previous studies. A notable feature in our study was normal electrophysiological testing results of the patient. Conclusions: In this study we reinforced the association between SYT2 mutations and distal hereditary motor neuropathy. We also described the clinical presentation of the patient carrying this pathogenic variant and provided unusual results of electrophysiological testing. The results showed that a diagnosis of SYT2-associated neuropathy should be based on the similarity of clinical manifestations, rather than the results of electrophysiological testing.
Mandibulofacial Dysostosis Attributed to a Recessive Mutation of CYP26C1 in Hereford Cattle
Authors: Sieck Renae L. , Fuller Anna M. , Bedwell Patrick S. , Ward Jack A. , Sanders Stacy K. , Xiang Shi-Hua , Peng Sichong , Petersen Jessica L. , Steffen David J.
Published: October 2020

In spring 2020, six Hereford calves presented with congenital facial deformities attributed to a condition we termed mandibulofacial dysostosis (MD). Affected calves shared hallmark features of a variably shortened and/or asymmetric lower mandible and bilateral skin tags present 2–10 cm caudal to the commissure of the lips. Pedigree analysis revealed a single common ancestor shared by the sire and dam of each affected calf. Whole-genome sequencing (WGS) of 20 animals led to the discovery of a variant (Chr26 g. 14404993T>C) in Exon 3 of CYP26C1 associated with MD. This missense mutation (p.L188P), is located in an α helix of the protein, which the identified amino acid substitution is predicted to break. The implication of this mutation was further validated through genotyping 2 additional affected calves, 760 other Herefords, and by evaluation of available WGS data from over 2500 other individuals. Only the affected individuals were homozygous for the variant and all heterozygotes had at least one pedigree tie to the suspect founder. CYP26C1 plays a vital role in tissue-specific regulation of retinoic acid (RA) during embryonic development. Dysregulation of RA can result in teratogenesis by altering the endothelin-1 signaling pathway affecting the expression of Dlx genes, critical to mandibulofacial development. We postulate that this recessive missense mutation in CYP26C1 impacts the catalytic activity of the encoded enzyme, leading to excess RA resulting in the observed MD phenotype.
(Human-Inflected) Evolution in an Age of (Human-Induced) Extinction: Synthetic Biology Meets the Anthropocene
Authors: Wodak Josh
Published: October 2020

At the advent of the Anthropocene, life is being pushed to its limits the world over; we are currently living through the Sixth Mass Extinction to occur since multicellular life first emerged on the planet 570 million years ago. Evolutionary biologist E.O. Wilson sums up this push in the opening gambit of his book The Future of Life: “the race is now on between the techno-scientific forces that are destroying the living environment and those that can be harnessed to save it”. Contra Wilson, this paper addresses the paradox arising from proposals to harness “techno-scientific forces … to save” the “living environment” while other forces continue to destroy it. By framing human-inflected evolution in an age of human-induced extinction, this article asks what could or should conservation become, if ‘conserving’ imperiled species might now require genetic interventions of the synthetic kind. Drawing upon recent key markers of “the race”, this paper presents a notional conservation for the Anthropocene—namely, that such a conservation proposes active intervention not only into ecosystems but into evolution itself. And yet, such interventions can only be considered in the context of the planetary scale that is the Anthropocene-writ-large, as per the desertification of the Amazon or the collapse of Antarctic ice sheets, the spatial scale of the microbial world, and on the temporal scale of evolution. Viewed within such a context, this paper presents technoscientific conservation as paradoxically being both vital and futile, as well as timely and too late.
Certificate-Of-Need Regulation and Healthcare Service Quality: Evidence from the Nursing Home Industry
Authors: Fayissa Bichaka , Alsaif Saleh , Mansour Fady , Leonce Tesa E. , Mixon Franklin G.
Published: October 2020

This quantitative study investigates the effect of certificate-of-need (CON) regulation on the quality of care in the nursing home industry. It uses county-level demographic data from the 48 contiguous US states that are extracted from the American Community Survey (ACS) and cover the years 2012, 2013, and 2014. In doing so, it employs a new set of service quality variables captured from a variety of county-level data sources. Instrumental variables results indicate that health survey scores for nursing homes that are computed by healthcare professionals are about 18–24% lower, depending on the type of nursing home under consideration, in states with CON regulation. We also find that the presence of CON regulation leads to a substitution of lower-quality certified nursing assistant care for higher-quality licensed practical nurse care, regardless of the type of nursing home under consideration.
Short Bouts of Physical Activity Are Associated with Reduced Smoking Withdrawal Symptoms, But Perceptions of Intensity May Be the Key
Authors: Masiero Marianna , Keyworth Helen , Pravettoni Gabriella , Cropley Mark , Bailey Alexis
Published: October 2020

The primary aim of this study was to assess the effectiveness of a short bout (10 min) of moderate-intensity exercise to reduce withdrawal symptomatology, craving and negative affect; while the secondary aim was to assess how the effectiveness of a short bout of moderate exercise can be modulated by the perception of intensity in physically active and low-activity smokers. Fifty low-activity and physically active smokers were recruited (24 male and 26 female) and randomized in three different conditions. Prescribed (objective) moderate intensity (OBJ) and perceived moderate intensity (PER), and passive waiting (PW). After the intervention (T3), smokers reported less desire to smoke in the PER (p < 0.001) and OBJ (p < 0.001) conditions, relative to the PW condition. At T3 smokers in the PER condition reported less negative affect than smokers in the PW condition relative to the baseline (T1) (p < 0.007). Further, smokers in the PER condition reported less negative affect than smokers in the PW condition (p < 0.048). Physically active (PA) smokers perceived less exertion than low-activity (LA) smokers, and the effects were stronger in the PER condition relative to OBJ. Generally, our results suggest that a short bout of moderate exercise helps both LA and PA smokers. These findings provided a novel insight into the psychological mechanisms that affect the efficacy of the exercise in smoking cessation and suggest that exercise should be tailored according to individual perception of intensity.
Meeting SDG6 in the Kingdom of Tonga: The Mismatch between National and Local Sustainable Development Planning for Water Supply
Authors: White Ian , Falkland Tony , Kula Taaniela
Published: October 2020

UN Sustainable Development Goal 6 challenges small island developing states such as the Kingdom of Tonga, which relies on variable rainwater and fragile groundwater lenses for freshwater supply. Meeting water needs in dispersed small islands under changeable climate and frequent extreme events is difficult. Improved governance is central to better water management. Integrated national sustainable development plans have been promulgated as a necessary improvement, but their relevance to island countries has been questioned. Tonga’s national planning instrument is the Tonga Strategic Development Framework, 2015–2025 (TSDFII). Local Community Development Plans (CDPs), developed by rural villages throughout Tonga’s five Island Divisions, are also available. Analyses are presented of island water sources from available census and limited hydrological data, and of the water supply priorities in TSDFII and in 117 accessible village CDPs. Census and hydrological data showed large water supply differences between islands. Nationally, TDSFII did not identify water supply as a priority. In CDPs, 84% of villages across all Island Divisions ranked water supply as a priority. Reasons for the mismatch are advanced. It is recommended that improved governance in water in Pacific Island countries should build on available census and hydrological data and increased investment in local island planning processes.
Validity and Reliability of the Satel 40 Hz Stabilometric Force Platform for Measuring Quiet Stance and Dynamic Standing Balance in Healthy Subjects
Authors: Rodríguez-Rubio Pere Ramón , Bagur-Calafat Caritat , López-de-Celis Carlos , Bueno-Gracía Elena , Cabanas-Valdés Rosa , Herrera-Pedroviejo Ernesto , Girabent-Farrés Montserrat
Published: October 2020

Background: A force platform must have validity and reliability for optimal use. The objective of this study was to analyze the validity and the reliability of the Satel 40 Hz stabilometric force platform. Methods: A study of instrumental validity and reliability, involving a cross-sectional correlational and comparative analysis was performed. To determine the validity, four certified weights located on three axes were used and the ability of the stabilometric force platform to detect changes in the position of the different axes was observed. A test–retest was performed to analyze the reliability. Forty-two symptom-free volunteers participated in the study. Assessments were taken in a standing static position and in a dynamic position, with the eyes open and closed. Three measurements were taken and the intra-class correlation coefficient (ICC) was calculated. Results: The validity increased as the weight increased for all the variables measured in the stabilometric parameters (p < 0.05). The reliability was shown to be good to excellent for the two visual conditions. The positional variables obtained a higher ICC. The variable with the best ICC was the Y mean in OE (ICC 0.874 and a p < 0.001). All the values showed an increase in a dynamic situation. Conclusion: The findings support the reliability and validity of the Satel 40 Hz stabilometric force platform. The platform could be recommended to evaluate static and dynamic standing balance in healthy adult individuals. Guidelines for treatment and the level of quality of stabilometry could be obtained from its use.
Metabolic and Cardiorespiratory Responses of Semiprofessional Football Players in Repeated Ajax Shuttle Tests and Curved Sprint Tests, and Their Relationship with Football Match Play
Authors: Gabrys Tomasz , Stanula Arkadiusz , Szmatlan-Gabrys Urszula , Garnys Michal , Charvát Luboš , Gupta Subir
Published: October 2020

In this study, the Ajax Shuttle Test (AST) and the Curved Sprint Test (CST) were conducted on semiprofessional football players to evaluate (1) their test performance, (2) the extent of anaerobic glycolysis by measuring blood lactate, (3) performance decrement and onset of fatigue, and (4) the correlation between selected physiological variables and test performance. Thirty-two semiprofessional Polish football players participated in this study. Both AST and CST were conducted on an outdoor football ground and were conducted in two sets; each set had six repetitions. In the case of AST, the total duration for 6 repetitions of the exercise in Sets 1 and 2 were 90.63 ± 3.71 and 91.65 ± 4.24 s, respectively, whereas, in the case of CST, the respective values were 46.8 ± 0.56 and 47.2 ± 0.66 s. Peak blood lactate concentration [La] after Sets 1 and 2 of AST were 14.47 ± 3.77 and 15.00 ± 1.85 mmol/L, and in the case of CST, the values were 8.17 ± 1.32 and 9.78 ± 1.35 mmol/L, respectively. Performance decrement in AST was more than in CST, both after Set 1 (4.32 ± 1.43 and 3.31 ± 0.96 in AST and CST, respectively) and Set 2 (7.95 ± 3.24 and 3.71 ± 1.02 in AST and CST, respectively). Only in a few of the repetitions, pulmonary ventilation (VE) and oxygen uptake (VO2) were found to be significantly correlated with the performance of the volunteers in both AST and CST. Respiratory exchange ratio (RER) was significantly correlated with most of the repetitions of AST, but not with CST. The study concludes that (1) AST shows more dependence on the anaerobic glycolytic system than shorter repetitive sprints (as in CST), (2) there is more performance decrement and fatigue in AST than in CST, and (3) early decrease in performance and fatigue in the semiprofessional football players in AST and CST may be due to the insufficiency of their aerobic energy system.
Managing Asthma and Obesity Related Symptoms (MATADORS): An mHealth Intervention to Facilitate Symptom Self-Management among Youth
Authors: Nichols Michelle , Teufel Ronald , Miller Sarah , Madisetti Mohan , Giovanni Christine San , Chike-Harris Katherine , Jones Lacy , Prentice Margaret , Ruggiero Kenneth , Kelechi Teresa
Published: October 2020

Youth with multi-morbidity (one or more chronic diseases) are at increased risk of further morbidity and early mortality as they enter their adult years. Recent increases in both asthma and obesity among youth have led to high health care utilization, increased health related complications, and expanded risks of subsequent cardiovascular disease burden. Common symptoms seen with asthma and obesity include fatigue, pain, depression, and anxiety. These symptoms can result in decreased physical activity, social isolation, and poor quality of life, which also may contribute to increased morbidity and mortality over time. Youth ages 10–17 are in a transitionary period where their overall health and disease management shifts from one of parental oversight to one where the youth gradually experience increased autonomy over their health and care management. Managing Asthma and Obesity Related Symptoms (MATADORS), is a mHealth technology-enhanced nurse-guided intervention that incorporates a novel mobile health application and motivational enhancement principles within a behavioral activation framework. Providing high-risk youth with strategies to enhance symptom self-management may result in decreased symptom prevalence, improved quality of life, and long-term reduction of cardiovascular morbidity and mortality as they move into adulthood. Moreover, developing low-cost, scalable tools with end-user input may facilitate promote early intervention and improved access to care, and reduce overall disease burden and healthcare costs.
Associations between Dark Triad and Ambivalent Sexism: Sex Differences among Adolescents
Authors: Navas María Patricia , Maneiro Lorena , Cutrín Olalla , Gómez-Fraguela Jose Antonio , Sobral Jorge
Published: October 2020

The Dark Triad traits (DT; Machiavellianism, psychopathy, and narcissism) have been repeatedly labeled as a constellation of traits that are characterized by a dishonest and self-focused approach to interpersonal relations. Personality psychologists suggest that these traits make some people more susceptible than others to intergroup bias, threat, and aggression. Thus, in order to delve into a psychological profile prone to accepting and justifying sexist attitudes, the aims of the current study were to analyze the presence of DT and sexist attitudes in a sample of 367 adolescents (Mage = 15.12, SD = 0.88; 50.1% males), find out the relationships that DT has with both hostile and benevolent sexism, and analyze the relevant differences between sexes in these relationships. The results indicated higher scores in DT and Ambivalent sexism for males. The correlations of Machiavellianism with psychopathy, and psychopathy with narcissism revealed significantly higher associations in males than females. The structural equation modeling of the bifactorial model, characterized by a global latent factor that encompasses the common characteristics of DT—along with the three specific factors of Machiavellianism, psychopathy, and narcissism—showed that the global latent factor of DT was related to both hostile and benevolent sexism in males and females. Singularly, narcissism was related to benevolent sexism in males, and psychopathy was related to hostile sexism in females. Finally, this research discusses the implications of these results on the implementation of positive models of interpersonal relationships in adolescence towards dating violence prevention.
Validity and Reliability of the Beck Anxiety Inventory (BAI) for Family Caregivers of Children with Cancer
Authors: Toledano-Toledano Filiberto , Moral de la Rubia José , Domínguez-Guedea Miriam Teresa , Nabors Laura A. , Barcelata-Eguiarte Blanca E. , Rocha-Pérez Eduardo , Luna David , Leyva-López Ahidée , Rivera-Rivera Leonor
Published: October 2020

Currently, information about the psychometric properties of the Beck Anxiety Inventory (BAI) in family caregivers of children with cancer is not available; thus, there is no empirical evidence of its validity and reliability to support its use in this population in Mexico or in other countries. This study examined the psychometric properties of the BAI in family caregivers of children with cancer and pursued four objectives: to determine the factor structure of the BAI, estimate its internal consistency reliability, describe the distribution of BAI scores and the level of anxiety in the sample and test its concurrent validity in relation to depression and resilience. This cross-sectional study was carried out with convenience sampling. A sociodemographic questionnaire, the BAI, the Beck Depression Inventory and the Measurement Scale of Resilience were administered to an incidental sample of 445 family caregivers of children with cancer hospitalized at the National Institute of Health in Mexico City. Confirmatory factor analysis using the maximum likelihood method was performed to determine the factor structure and exploratory factor analysis using axis factorization with oblique rotation was conducted. The two-, three- and four-factor models originally proposed for the BAI did not hold. The exploratory factor analysis showed a model of two correlated factors (physiological and emotional symptoms). Confirmatory factor analysis revealed a lack of discriminant validity between these two factors and supported a single-factor model. The internal consistency of the scale reduced to 11 items (BAI-11) was good (alpha = 0.89). The distribution of BAI-11 scores was skewed to the left. High levels of symptoms of anxiety were present in 49.4% of caregivers. The scale was positively correlated with depression and negatively correlated with resilience. These findings suggest that a reduced single-factor version of the BAI is valid for Mexican family caregivers of children with cancer.
Compensations of Top Executives and M&A Behaviors: An Empirical Study of Listed Companies
Authors: Xue Jiao , Fan Heng , Dong Zhanxun
Published: October 2020

This study empirically examines the relationship between executive compensation and mergers and acquisitions (M&A) behaviors by identifying the influence of short- and long-term incentive on the propensity and scale of M&A. When the short-term incentive is insufficient, M&A behaviors serve as a beneficial compensation mechanism. Thus, lack of executives’ incentive promotes the propensity to engage in M&A and significantly affects the scale of M&A. With regard to long-term incentives, M&A behaviors serve as a beneficial creation mechanism. Shareholding of executives promotes M&A propensity, and does not significantly affect the scale of M&A. This study significantly contributes to research in M&A behaviors by revealing the beneficial distribution mechanisms of M&A behaviors.
How Urban Factors Affect the Spatiotemporal Distribution of Infectious Diseases in Addition to Intercity Population Movement in China
Authors: Niu Xinyi , Yue Yufeng , Zhou Xingang , Zhang Xiaohu
Published: October 2020

The outbreak of the 2019 novel coronavirus (COVID-19) has attracted global attention. During the Chinese New Year holiday, population outflow from Wuhan induced the spread of the epidemic to other cities in China. This study analyzed massive intercity movement data from Baidu and epidemic data to study how intercity population outflows affected the spatiotemporal spread of the epidemic. This study further investigated how urban factors influenced the spatiotemporal spread of COVID-19. The analysis indicates that intercity movement was an important factor in the spread of the epidemic in China, and the impact of intercity movement on the spread was heterogeneous across different classes of cities. The spread of the epidemic also varied among cities and was affected by urban factors including the total population, population density, and gross domestic product (GDP). The findings have implications for public health management. Mega-cities should consider tougher measures to contain the spread of the epidemic compared with other cities. It is of great significance for policymakers in any nation to assess the potential risk of epidemics and make cautious plans ahead of time.
A New Urban Vitality Analysis and Evaluation Framework Based on Human Activity Modeling Using Multi-Source Big Data
Authors: Liu Shaojun , Zhang Ling , Long Yi , Long Yao , Xu Mianhao
Published: October 2020

A quantitative study of urban vitality brings new insights for evaluating the external construction environment and internal development power of cities. However, it still has limited knowledge of the relations between people’s diverse urban life and urban vitality, although urban activities are often used as the proxy for urban vitality. This paper aims to deeply mine the content of urban social life and reveal the driving mechanism of urban vitality after inspecting human activities. We propose a general framework for exploring the spatial pattern and driving mechanism of urban vitality using multi-source big data. It builds a mapping relationship between various urban activities and urban vitality aspects, including economic and social. In addition, the physical environment (static) and human–land interaction (dynamic) indicators are designed to analyze the driving mechanism of urban vitality using the Geographically Weighted Regression model. The results show that the spatial pattern and driving factors of urban vitality are heterogeneous over space regarding both the economic and social aspects of our experimental study. This work provides us with multiple perspectives to understand the connotation of urban vitality and urges us to develop rational strategies to make the city more vital, coordinated, and sustainable.
LionVu 2.0 Usability Assessment for Pennsylvania, United States
Authors: Geyer Nathaniel R. , Kessler Fritz C. , Lengerich Eugene J.
Published: October 2020

The Penn State Cancer Initiative implemented LionVu 1.0 (Penn State University, United States) in 2017 as a web-based mapping tool to educate and inform public health professionals about the cancer burden in Pennsylvania and 28 counties in central Pennsylvania, locally known as the catchment area. The purpose of its improvement, LionVu 2.0, was to assist investigators answer person–place–time questions related to cancer and its risk factors by examining several data variables simultaneously. The primary objective of this study was to conduct a usability assessment of a prototype of LionVu 2.0 which included area- and point-based data. The assessment was conducted through an online survey; 10 individuals, most of whom had a masters or doctorate degree, completed the survey. Although most participants had a favorable view of LionVu 2.0, many had little to no experience with web mapping. Therefore, it was not surprising to learn that participants wanted short 10–15-minute training videos to be available with future releases, and a simplified user-interface that removes advanced functionality. One unexpected finding was the suggestion of using LionVu 2.0 for teaching and grant proposals. The usability study of the prototype of LionVu 2.0 provided important feedback for its future development.
Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia
Authors: Rosa-Caldwell Megan E. , Benson Conner A. , Lee David E. , Brown Jacob L. , Washington Tyrone A. , Greene Nicholas P. , Wiggs Michael P.
Published: October 2020

It is established that cancer cachexia causes limb muscle atrophy and is strongly associated with morbidity and mortality; less is known about how the development of cachexia impacts the diaphragm. The purpose of this study was to investigate cellular signaling mechanisms related to mitochondrial function, reactive oxygen species (ROS) production, and protein synthesis during the development of cancer cachexia. C57BL/J6 mice developed Lewis Lung Carcinoma for either 0 weeks (Control), 1 week, 2 weeks, 3 weeks, or 4 weeks. At designated time points, diaphragms were harvested and analyzed. Mitochondrial respiratory control ratio was ~50% lower in experimental groups, which was significant by 2 weeks of cancer development, with no difference in mitochondrial content markers COXIV or VDAC. Compared to the controls, ROS was 4-fold elevated in 2-week animals but then was not different at later time points. Only one antioxidant protein, GPX3, was altered by cancer development (~70% lower in experimental groups). Protein synthesis, measured by a fractional synthesis rate, appeared to become progressively lower with the cancer duration, but the mean difference was not significant. The development and progression of cancer cachexia induces marked alterations to mitochondrial function and ROS production in the diaphragm and may contribute to increased cachexia-associated morbidity and mortality.
Cadmium-Induced Cell Homeostasis Impairment is Suppressed by the Tor1 Deficiency in Fission Yeast
Authors: Požgajová Miroslava , Navrátilová Alica , Šebová Eva , Kovár Marek , Kačániová Miroslava
Published: October 2020

Cadmium has no known physiological function in the body; however, its adverse effects are associated with cancer and many types of organ system damage. Although much has been shown about Cd toxicity, the underlying mechanisms of its responses to the organism remain unclear. In this study, the role of Tor1, a catalytic subunit of the target of rapamycin complex 2 (TORC2), in Cd-mediated effects on cell proliferation, the antioxidant system, morphology, and ionome balance was investigated in the eukaryotic model organism Schizosaccharomyces pombe. Surprisingly, spectrophotometric and biochemical analyses revealed that the growth rate conditions and antioxidant defense mechanisms are considerably better in cells lacking the Tor1 signaling. The malondialdehyde (MDA) content of Tor1-deficient cells upon Cd treatment represents approximately half of the wild-type content. The microscopic determination of the cell morphological parameters indicates the role for Tor1 in cell shape maintenance. The ion content, determined by inductively coupled plasma optical emission spectroscopy (ICP-OES), showed that the Cd uptake potency was markedly lower in Tor1-depleted compared to wild-type cells. Conclusively, we show that the cadmium-mediated cell impairments in the fission yeast significantly depend on the Tor1 signaling. Additionally, the data presented here suggest the yet-undefined role of Tor1 in the transport of ions.
Precise Editing of the OsPYL9 Gene by RNA-Guided Cas9 Nuclease Confers Enhanced Drought Tolerance and Grain Yield in Rice (Oryza sativa L.) by Regulating Circadian Rhythm and Abiotic Stress Responsive Proteins
Authors: Usman Babar , Nawaz Gul , Zhao Neng , Liao Shanyue , Liu Yaoguang , Li Rongbai
Published: October 2020

Abscisic acid (ABA) is involved in regulating drought tolerance, and pyrabactin resistance-like (PYL) proteins are known as ABA receptors. To elucidate the role of one of the ABA receptors in rice, OsPYL9 was mutagenized through CRISPR/Cas9 in rice. Homozygous and heterozygous mutant plants lacking any off-targets and T-DNA were screened based on site-specific sequencing and used for morpho-physiological, molecular, and proteomic analysis. Mutant lines appear to accumulate higher ABA, antioxidant activities, chlorophyll content, leaf cuticular wax, and survival rate, whereas a lower malondialdehyde level, stomatal conductance, transpiration rate, and vascular bundles occur under stress conditions. Proteomic analysis found a total of 324 differentially expressed proteins (DEPs), out of which 184 and 140 were up and downregulated, respectively. The OsPYL9 mutants showed an increase in grain yield under both drought and well watered field conditions. Most of the DEPs related to circadian clock rhythm, drought response, and reactive oxygen species were upregulated in the mutant plants. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEPs were only involved in circadian rhythm and Gene Ontology (GO) analysis showed that most of the DEPs were involved in response to abiotic stimulus, and abscisic acid-activated signaling pathways. Protein GIGANTEA, Adagio-like, and Pseudo-response regulator proteins showed higher interaction in protein–protein interaction (PPI) network. Thus, the overall results showed that CRISPR/Cas9-generated OsPYL9 mutants have potential to improve both drought tolerance and the yield of rice. Furthermore, global proteome analysis provides new potential biomarkers and understandings of the molecular mechanism of rice drought tolerance.
BacSJ—Another Bacteriocin with Distinct Spectrum of Activity that Targets Man-PTS
Authors: Tymoszewska Aleksandra , Walczak Piotr , Aleksandrzak-Piekarczyk Tamara
Published: October 2020

Lactic acid bacteria produce diverse antimicrobial peptides called bacteriocins. Most bacteriocins target sensitive bacteria by binding to specific receptors. Although a plethora of bacteriocins have been identified, for only a few of them the receptors they recognize are known. Here, we identified permease IIC and surface protein IID, two membrane subunits of the mannose-specific quaternary phosphotransferase system (Man-PTS), as a receptor for BacSJ, a subclass IId bacteriocin produced by Lactobacillus paracasei subsp. paracasei BGSJ2-8. BacSJ shares 45% identity with another Man-PTS binding bacteriocin, garvicin Q (GarQ). Similarly to GarQ, BacSJ has a relatively broad activity spectrum acting against several Gram-positive bacteria, such as Lactococcus lactis and Listeria monocytogenes, harboring fairly similar Man-PTSs, but not against Lactococcus garvieae. To identify specific Man-PTS amino acids responsible for the L.lactis sensitivity to BacSJ, and thus likely involved in the interaction with this bacteriocin, we generated eight independent BacSJ resistant L.lactis mutants harboring five distinct missense mutations in the ptnC or ptnD genes encoding the IIC and IID subunits. Concurrently with the resistance to BacSJ, the mutants efficiently utilized mannose as a carbon source, which indicated functionality of their mutated Man-PTS. The amino acid substitutions in the mutants localized to the intracellular region of the IIC permease or to the extracellular parts of IID. This localization coincides with regions targeted by GarQ and some other Man-PTS-binding garvicins, pointing to similarities between all these bacteriocins in the mechanism of their interaction with Man-PTS. During the attack by these bacteriocins, subunits IID and IIC are assumed to function sequentially as a docking and an entry module allowing the toxic peptide to bind the cell and then open the pore. However, since not all of the BacSJ-resistant mutants exhibited cross-resistance to GarQ, we propose that BacSJ interacts with Man-PTS in a manner slightly different from that of GarQ.
Newborn Screening for X-Linked Adrenoleukodystrophy in Georgia: Experiences from a Pilot Study Screening of 51,081 Newborns
Authors: Hall Patricia L. , Li Hong , Hagar Arthur F. , Jerris S. Caleb , Wittenauer Angela , Wilcox William
Published: October 2020

We screened 51,081 newborns for X-linked adrenoleukodystrophy (ALD) using a two-tiered strategy quantifying very long chain lysophosphatadylcholines (LPC). Our testing strategy used flow injection tandem mass spectrometry for the first-tier analysis of LPCs, and second-tier quantification of C26:0 LPC using liquid chromatography tandem mass spectrometry. There were 364 specimens considered abnormal using our first-tier algorithm that relied on the four LPC measurements and post-analytical tools. Second-tier test results were reported as normal or abnormal based on a cutoff for the single analyte, C26:0 LPC. Eleven cases were reported as abnormal based on second-tier test results. One male with ALD was identified, and two females with peroxisomal biogenesis disorders were also identified. A single female case remains unresolved, due to a loss to follow up after a negative molecular test result for ABCD1 gene sequencing. The positive predictive value for confirmed, clinically relevant disorders during this pilot study was 27.3%. Challenges identified during the study period were based around coverage for confirmatory testing, particularly if family members needed molecular testing, which is an ongoing issue with newborn screening in Georgia. We also encountered issues with the follow up for a patient who remained asymptomatic. Due to the different timelines involved with clinical findings in ALD, follow-up coordination may be more difficult, particularly if the child identified by newborn screening (NBS) is the only member of the family affected, or able to be tested.
Distinct Expression Patterns of VEGFR 1-3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Supporting Clinical Relevance, but not a Prognostic Factor
Authors: Bösch Florian , Altendorf-Hofmann Annelore , Jacob Sven , Auernhammer Christoph J. , Spitzweg Christine , Boeck Stefan , Schubert-Fritschle Gabriele , Werner Jens , Kirchner Thomas , Angele Martin K. , Knösel Thomas
Published: October 2020

Introduction: Gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) are an increasing tumor entity. Since many patients are diagnosed at an advanced stage, treatment is still challenging and dependent on many tumor and patient specific factors. Therefore, the aim of the present study was to elucidate the expression rates and the prognostic value of vascular endothelial growth factor receptor (VEGFR) 1-3 in GEP-NENs. A potential association to immune checkpoint markers was further investigated. Material and Methods: The expression levels of VEGFR 1-3 were analyzed by immunohistochemistry and correlated with the expression of the checkpoint markers PD-1 and PD-L1. Furthermore, the tumor samples of 249 GEP-NEN patients were studied and correlated with overall survival rates and clinicopathological features. Kaplan–Meier analyses and the log rank test were used for survival analyses. Categorical variables were compared by the χ2 test. Results: The most common primary tumor site was the small intestine (50.6%), followed by the pancreas (25.7%). VEGFR 1 was highly expressed in 59%, VEGFR 2 in 6.4%, and VEGFR 3 in 61.8% of the analyzed samples. The expression of VEGFR 1-3 was not significantly associated with survival rates. Pancreatic NENs had the highest expression of VEGFR 1 and 3 in 80% of the cases. VEGFR 1-3 positivity correlated with the expression levels of immune checkpoint markers. Discussion: VEGFR 1-3 show a distinct expression pattern in different subgroups of neuroendocrine neoplasias indicating a conceivable target. Moreover, there was a substantial association between VEGFRs and immune checkpoint markers. Taken together, anti-VEGFR therapy represents a promising therapeutic approach in GEP-NEN patients and should be addressed in future studies.
Clinical Application of Virtual Reality for Upper Limb Motor Rehabilitation in Stroke: Review of Technologies and Clinical Evidence
Authors: Kim Won-Seok , Cho Sungmin , Ku Jeonghun , Kim Yuhee , Lee Kiwon , Hwang Han-Jeong , Paik Nam-Jong
Published: October 2020

Neurorehabilitation for stroke is important for upper limb motor recovery. Conventional rehabilitation such as occupational therapy has been used, but novel technologies are expected to open new opportunities for better recovery. Virtual reality (VR) is a technology with a set of informatics that provides interactive environments to patients. VR can enhance neuroplasticity and recovery after a stroke by providing more intensive, repetitive, and engaging training due to several advantages, including: (1) tasks with various difficulty levels for rehabilitation, (2) augmented real-time feedback, (3) more immersive and engaging experiences, (4) more standardized rehabilitation, and (5) safe simulation of real-world activities of daily living. In this comprehensive narrative review of the application of VR in motor rehabilitation after stroke, mainly for the upper limbs, we cover: (1) the technologies used in VR rehabilitation, including sensors; (2) the clinical application of and evidence for VR in stroke rehabilitation; and (3) considerations for VR application in stroke rehabilitation. Meta-analyses for upper limb VR rehabilitation after stroke were identified by an online search of Ovid-MEDLINE, Ovid-EMBASE, the Cochrane Library, and KoreaMed. We expect that this review will provide insights into successful clinical applications or trials of VR for motor rehabilitation after stroke.
Ischemic and Bleeding Events Associated with Thrombocytopenia and Thrombocytosis after Percutaneous Coronary Intervention in Patients with Acute Myocardial Infarction
Authors: Roh Ji Woong , Lim Sungmin , Hwang Youngdeok , Lee Kwan Yong , Choo Eun Ho , Choi Ik Jun , Hwang Byung-Hee , Kim Chan Joon , Park Mahn-Won , Kim Dong-Bin , Lee Jong-Min , Park Chul Soo , Kim Hee-Yeol , Yoo Ki-Dong , Jeon Doo Soo , Youn Ho Joong , Chung Wook Sung , Kim Min Chul , Jeong Myung Ho , Ahn Youngkeun , Chang Kiyuk
Published: October 2020

The early and late ischemic and bleeding clinical outcomes according to baseline platelet count after percutaneous coronary intervention (PCI) in patients with acute myocardial infarction (AMI) remain unclear. Overall, 10,667 patients from the Cardiovascular Risk and identification of potential high-risk population in AMI (COREA-AMI) I and II registries were classified according to the following universal criteria on baseline platelet counts: (1) moderate to severe thrombocytopenia (platelet < 100 K/μL, n = 101), (2) mild thrombocytopenia (platelet = 100~149 K/μL, n = 631), (3) normal reference (platelet = 150~450 K/μL, n = 9832), and (4) thrombocytosis (platelet > 450 K/μL, n = 103). The primary endpoint was the occurrence of major adverse cardiovascular events (MACE). The secondary outcome was Bleeding Academic Research Consortium (BARC) 2, 3, and 5 bleeding. After adjusting for confounders, the moderate to severe thrombocytopenia (HR, 2.03; 95% CI, 1.49–2.78); p < 0.001), mild thrombocytopenia (HR, 1.15; 95% CI, 1.01–1.34; p = 0.045), and thrombocytosis groups (HR, 1.47; 95% CI, 1.07–2.03; p = 0.019) showed higher 5-year MACE rates than the normal reference. In BARC 2, 3, and 5 bleeding outcomes, the bleedings rates were higher than the normal range in the moderate to severe thrombocytopenia (HR, 2.18; 95% CI, 1.36–3.49; p = 0.001) and mild thrombocytopenia (HR, 1.41; 95% CI, 1.12–1.78; p = 0.004) groups. Patients with AMI had higher 5-year MACE rates after PCI if they had lower- or higher-than-normal platelet counts. Thrombocytopenia revealed higher early and late bleeding rates whereas thrombocytosis showed long-term bleeding trends, although these trends were not statistically significant.