bluuebunny/crossref_metadata_2025 huggingface dataset:
In-Service Thermal and Luminous Performance Monitoring of a Refurbished Building with Solar Control Films on the Glazing System
Authors: Teixeira Henriqueta , Gomes Maria da Glória , Moret Rodrigues António , Pereira Júlia
Published: March 2021

The global increase in energy needs and environmental awareness for a more efficient energy use have boosted building rehabilitation to decrease energy consumption. The installation of solar control films (SCFs) in buildings with large glazing façades makes it possible to reduce excessive solar gains through the glazing. The main purpose of the work is to assess, with field experimental data, the thermal and luminous performances of double-glazing units with SCFs installed in office rooms, in Lisbon. An experimental campaign was carried out simultaneously in three adjacent offices: one with a highly reflective SCF (external installation), one with a reflective SCF (internal installation) and one without an SCF. The exterior SCF showed the best thermal performance with reductions in the peak indoor air temperature of up to 6.9 and 2.3 °C during the representative days of the heating and cooling periods, respectively, increasing thermal comfort mainly during the cooling period. The interior SCF had a poorer thermal performance since it contributed to solar radiation absorption that is then emitted as heat into the indoor environment, increasing the greenhouse effect of the office. The presence of SCFs reduced the indoor illuminance levels, having a positive impact on thermal comfort and glare reduction in the cooling period.
Application of Liquid Hydrogen Carriers in Hydrogen Steelmaking
Authors: Andersson Joakim
Published: March 2021

Steelmaking is responsible for approximately one third of total industrial carbon dioxide (CO2) emissions. Hydrogen (H2) direct reduction (H-DR) may be a feasible route towards the decarbonization of primary steelmaking if H2 is produced via electrolysis using fossil-free electricity. However, electrolysis is an electricity-intensive process. Therefore, it is preferable that H2 is predominantly produced during times of low electricity prices, which is enabled by the storage of H2. This work compares the integration of H2 storage in four liquid carriers, methanol (MeOH), formic acid (FA), ammonia (NH3) and perhydro-dibenzyltoluene (H18-DBT), in H-DR processes. In contrast to conventional H2 storage methods, these carriers allow for H2 storage in liquid form at moderate overpressures, reducing the storage capacity cost. The main downside to liquid H2 carriers is that thermochemical processes are necessary for both the storage and release processes, often with significant investment and operational costs. The carriers are compared using thermodynamic and economic data to estimate operational and capital costs in the H-DR context considering process integration options. It is concluded that the use of MeOH is promising compared to the other considered carriers. For large storage volumes, MeOH-based H2 storage may also be an attractive option to the underground storage of compressed H2. The other considered liquid H2 carriers suffer from large thermodynamic barriers for hydrogenation (FA) or dehydrogenation (NH3, H18-DBT) and higher investment costs. However, for the use of MeOH in an H-DR process to be practically feasible, questions regarding process flexibility and the optimal sourcing of CO2 and heat must be answered.
A Capabilities-Led Approach to Assessing Technological Solutions for a Rural Community
Authors: Wang Xinfang , Day Rosie , Murrant Dan , Marín Antonio Diego , Botello David Castrejón , González Francisco López , Radcliffe Jonathan
Published: March 2021

To improve access to affordable, reliable and sustainable energy in rural areas of the global south, off-grid systems using renewable generation and energy storage are often proposed. However, solution design is often technology-driven, with insufficient consideration of social and cultural contexts. This leads to a risk of unintended consequences and inappropriate systems that do not meet local needs. To address this problem, this paper describes the application of a capabilities-led approach to understanding a community’s multi-dimensional energy poverty and assessing their needs as they see them, in order to better design suitable technological interventions. Data were collected in Tlamacazapa, Mexico, through site visits and focus groups with men and women. These revealed the ways in which constrained energy services undermined essential capabilities, including relating to health, safety, relationships and earning a living, and highlighted the specific ways in which improved energy services, such as lighting, cooking and mechanical power could improve capabilities in the specific context of Tlamacazapa. Based on these findings, we propose some potential technological interventions to address these needs. The case study offers an illustration of an assessment method that could be deployed in a variety of contexts to inform the design of appropriate technological interventions.
A Deep Learning Approach to Downscale Geostationary Satellite Imagery for Decision Support in High Impact Wildfires
Authors: McCarthy Nicholas F. , Tohidi Ali , Aziz Yawar , Dennie Matt , Valero Mario Miguel , Hu Nicole
Published: March 2021

Scarcity in wildland fire progression data as well as considerable uncertainties in forecasts demand improved methods to monitor fire spread in real time. However, there exists at present no scalable solution to acquire consistent information about active forest fires that is both spatially and temporally explicit. To overcome this limitation, we propose a statistical downscaling scheme based on deep learning that leverages multi-source Remote Sensing (RS) data. Our system relies on a U-Net Convolutional Neural Network (CNN) to downscale Geostationary (GEO) satellite multispectral imagery and continuously monitor active fire progression with a spatial resolution similar to Low Earth Orbit (LEO) sensors. In order to achieve this, the model trains on LEO RS products, land use information, vegetation properties, and terrain data. The practical implementation has been optimized to use cloud compute clusters, software containers and multi-step parallel pipelines in order to facilitate real time operational deployment. The performance of the model was validated in five wildfires selected from among the most destructive that occurred in California in 2017 and 2018. These results demonstrate the effectiveness of the proposed methodology in monitoring fire progression with high spatiotemporal resolution, which can be instrumental for decision support during the first hours of wildfires that may quickly become large and dangerous. Additionally, the proposed methodology can be leveraged to collect detailed quantitative data about real-scale wildfire behaviour, thus supporting the development and validation of fire spread models.
Fluid-Structure Interaction of Flexible Whisker-Type Beams and Its Implications for Flow Sensing by Pair-Wise Correlation
Authors: Glick Raphael , Muthuramalingam Muthukumar , Brücker Christoph
Published: March 2021

(1) Background: Sensing of critical events or flow signatures in nature often presents itself as a coupled interaction between a fluid and arrays of slender flexible beams, such a wind-hairs or whiskers. It is hypothesized that important information is gained in highly noisy environments by the inter-correlation within the array. (2) Methods: The present study uses a model sea lion head with artificial whiskers in the form of slender beams (optical fibres), which are subjected to a mean flow with overlaid turbulent structures generated in the wake of a cylinder. Motion tracking of the array of fibres is used to analyse the correlation of the bending deformations of pairs of fibres. (3) Results: Cross-correlation of the bending signal from tandem pairs of whiskers proves that the detection of vortices and their passage along the animals head is possible even in noisy environments. The underlying pattern, during passage of a vortex core, is a jerk-like response of the whiskers, which can be found at later arrival-times in similar form in the downstream whisker’s response. (4) Conclusions: Coherent vortical structures can be detected from cross-correlation of pairs of cantilever-beam like sensors even in highly turbulent flows. Such vortices carry important information within the environment, e.g., the underlying convection velocity. More importantly in nature, these vortices are characteristic elementary signals left by prey and predators. The present work can help to further develop flow, or critical event, sensory systems which can overcome high noise levels due to the proposed correlation principle.
Functional and Quality Characteristics of Ginger, Pineapple, and Turmeric Juice Mix as Influenced by Blend Variations
Authors: Ogori Akama Friday , Amove Julius , Aduloju Precious , Sardo Giacomo , Okpala Charles Odilichukwu R. , Bono Gioacchino , Korzeniowska Małgorzata
Published: March 2021

In this current work, the functional and quality characteristics of ginger, pineapple, and turmeric juice mix as influenced by blend variations were investigated. Specifically, the blends had constant ginger amounts, decreased pineapple, and increased turmeric proportionally. Additionally, the functional properties involved physicochemical (pH, soluble solids (SS), total titratable acidity (TA) and viscosity), proximate (moisture, protein, fat and ash), minerals (Ca, and Mg) and vitamin C and β-carotene analyses, whereas quality properties involved microbiological and sensory analyses. The results showed that as quantities of pineapple and turmeric respectively decreased and increased, there was significant increases in Ca, Mg, vitamin C, and β-carotene contents (p < 0.05). Across the blends, the degree of significant differences (p < 0.05) in the protein, fat, and ash seemed more compared to those of moisture contents. Despite the increases in pH and viscosity, and decreases in SS and TA, the increases in turmeric potentially reinforced by ginger most likely decreased the bacterial/fungi counts, as well as inhibition zones. Increasing and decreasing the respective amounts of turmeric and pineapple might not necessarily make the blends more acceptable, given the decreases in appearance, taste, aroma, and mouthfeel scores.
Differentiation between Fresh and Thawed Cephalopods Using NIR Spectroscopy and Multivariate Data Analysis
Authors: Pennisi Francesco , Giraudo Alessandro , Cavallini Nicola , Esposito Giovanna , Merlo Gabriele , Geobaldo Francesco , Acutis Pier Luigi , Pezzolato Marzia , Savorani Francesco , Bozzetta Elena
Published: March 2021

The sale of frozen–thawed fish and fish products, labeled as fresh, is currently one of the most common and insidious commercial food frauds. For this reason, the demand of reliable tools to identify the storage conditions is increasing. The present study was performed on two species, commonly sold in large-scale distribution: Cuttlefish (Sepia officinalis) and musky octopus (Eledone spp.). Fifty fresh cephalopod specimens were analyzed at refrigeration temperature (2 ± 2 °C), then frozen at −20 °C for 10 days and finally thawed and analyzed again. The performance of three near-infrared (NIR) instruments in identifying storage conditions were compared: The benchtop NIR Multi Purpose Analyzer (MPA) by Bruker, the portable MicroNIR by VIAVI and the handheld NIR SCiO by Consumer Physics. All collected spectra were processed and analyzed with chemometric methods. The SCiO data were also analyzed using the analytical tools available in the online application provided by the manufacturer to evaluate its performance. NIR spectroscopy, coupled with chemometrics, allowed discriminating between fresh and thawed samples with high accuracy: Cuttlefish between 82.3–94.1%, musky octopus between 91.2–97.1%, global model between 86.8–95.6%. Results show how food frauds could be detected directly in the marketplace, through small, ultra-fast and simplified handheld devices, whereas official control laboratories could use benchtop analytical instruments, coupled with chemometric approaches, to develop accurate and validated methods, suitable for regulatory purposes.
Strategies to Improve the Clinical Outcomes for Direct-to-Consumer Pharmacogenomic Tests
Authors: Tafazoli Alireza , Guggilla Rama Krishna , Kamel-Koleti Zahra , Miltyk Wojciech
Published: March 2021

Direct-to-consumer genetic tests (DTC-GT) have become a bridge between marketing and traditional healthcare services. After earning FDA endorsement for such facilities, several fast-developing companies started to compete in the related area. Pharmacogenomic (PGx) tests have been introduced as potentially one of the main medical services of such companies. Most of the individuals will be interested in finding out about the phenotypic consequences of their genetic variants and molecular risk factors against diverse medicines they take or will take later. Direct-to-consumer pharmacogenomic tests (DTC-PT) is still in its young age, however it is expected to expand rapidly through the industry in the future. The result of PGx tests could be considered as the main road toward the implementation of personalized and precision medicine in the clinic. This narrative critical review study provides a descriptive overview on DTC-GT, then focuses on DTC-PT, and also introduces and suggests the potential approaches for improving the clinical related outcomes of such tests on healthcare systems.
The Osa-Containing SWI/SNF Chromatin-Remodeling Complex Is Required in the Germline Differentiation Niche for Germline Stem Cell Progeny Differentiation
Authors: Hu Xiaolong , Li Mengjie , Hao Xue , Lu Yi , Zhang Lei , Wu Geng
Published: March 2021

The Drosophila ovary is recognized as a powerful model to study stem cell self-renewal and differentiation. Decapentaplegic (Dpp) is secreted from the germline stem cell (GSC) niche to activate Bone Morphogenic Protein (BMP) signaling in GSCs for their self-renewal and is restricted in the differentiation niche for daughter cell differentiation. Here, we report that Switch/sucrose non-fermentable (SWI/SNF) component Osa depletion in escort cells (ECs) results in a blockage of GSC progeny differentiation. Further molecular and genetic analyses suggest that the defective germline differentiation is partially attributed to the elevated dpp transcription in ECs. Moreover, ectopic Engrailed (En) expression in osa-depleted ECs partially contributes to upregulated dpp transcription. Furthermore, we show that Osa regulates germline differentiation in a Brahma (Brm)-associated protein (BAP)-complex-dependent manner. Additionally, the loss of EC long cellular processes upon osa depletion may also partly contribute to the germline differentiation defect. Taken together, these data suggest that the epigenetic factor Osa plays an important role in controlling EC characteristics and germline lineage differentiation.
Factors Influencing Pregnant Women’s Injuries and Fetal Loss Due to Motor Vehicle Collisions: A National Crash Data-Based Study
Authors: Hattori Shinobu , Hitosugi Masahito , Moriguchi Shingo , Baba Mineko , Takaso Marin , Nakamura Mami , Tsujimura Seiji , Miyata Yasuhito
Published: March 2021

To examine the factors that influence substantial injuries for pregnant women and negative fetal outcomes in motor vehicle collisions (MVCs), a retrospective analysis using the National Automotive Sampling System/Crashworthiness Data System was performed in Shiga University of Medical Science. We analyzed data from 736 pregnant women who, between 2001 and 2015, had injuries that were an abbreviated injury scale (AIS) score of one or more. The mean age was 25.9 ± 6.4 years and the mean gestational age was 26.2 ± 8.2 weeks. Additionally, 568 pregnant women had mild injuries and 168 had moderate to severe injuries. Logistic regression analysis revealed that seatbelt use (odds ratio (OR), 0.30), airbag deployment (OR, 2.00), and changes in velocity (21–40 km/h: OR, 3.03; 41–60 km/h: OR, 13.47; ≥61 km/h: OR, 44.56) were identified as independent predictors of having a moderate to severe injury. The positive and negative outcome groups included 231 and 12 pregnant women, respectively. Injury severity in pregnant women was identified as an independent predictor of a negative outcome (OR, 2.79). Avoiding moderate to severe maternal injuries is a high priority for saving the fetus, and education on appropriate seatbelt use and limiting vehicle speed for pregnant women is required.
Father’s Knowledge, Attitude and Support to Mother’s Exclusive Breastfeeding Practices in Bangladesh: A Multi-Group Structural Equations Model Analysis
Authors: Ouyang Yan-Qiong , Nasrin Layla
Published: March 2021

Despite worldwide initiatives, the exclusive breastfeeding (EBF) rate is low. The study aims to investigate the role of fathers’ knowledge, attitude and support in formulating mothers’ practice of breastfeeding taking Bangladeshi parental cases as a sample. The study uses a standard survey instrument following the theory of planned behavior (TPB). Responses from 332 couples are accepted following a standard criteria and used for analysis. The sample is divided into sub-groups based on delivery mode—vaginal (n = 211, 64%) and cesarean section (n = 121, 36%). Based on the sub-groups, a multi-group structural equation modelling (SEM) is applied to analyze the phenomena. The study finds that a father’s knowledge in EBF can, in one way, significantly enhance mother’s knowledge by sharing and, in another way, can enhance his own attitude to offer different support to his partner/wife which induces the chances of EBF practices by mothers. The enhanced EBF knowledge of a mother raises her attitude to practice EBF, which is also positively affected by the father’s attitude. Thus, fathers’ support, coupled with mothers’ positive attitude, the mother’s intention to practice EBF as per standard guidelines.
Positive Externalities of Climate Change Mitigation and Adaptation for Human Health: A Review and Conceptual Framework for Public Health Research
Authors: Bikomeye Jean C. , Rublee Caitlin S. , Beyer Kirsten M. M.
Published: March 2021

Anthropogenic climate change is adversely impacting people and contributing to suffering and increased costs from climate-related diseases and injuries. In responding to this urgent and growing public health crisis, mitigation strategies are in place to reduce future greenhouse gas emissions (GHGE) while adaptation strategies exist to reduce and/or alleviate the adverse effects of climate change by increasing systems’ resilience to future impacts. While these strategies have numerous positive benefits on climate change itself, they also often have other positive externalities or health co-benefits. This knowledge can be harnessed to promote and improve global public health, particularly for the most vulnerable populations. Previous conceptual models in mitigation and adaptation studies such as the shared socioeconomic pathways (SSPs) considered health in the thinking, but health outcomes were not their primary intention. Additionally, existing guidance documents such as the World Health Organization (WHO) Guidance for Climate Resilient and Environmentally Sustainable Health Care Facilities is designed primarily for public health professionals or healthcare managers in hospital settings with a primary focus on resilience. However, a detailed cross sectoral and multidisciplinary conceptual framework, which links mitigation and adaptation strategies with health outcomes as a primary end point, has not yet been developed to guide research in this area. In this paper, we briefly summarize the burden of climate change on global public health, describe important mitigation and adaptation strategies, and present key health benefits by giving context specific examples from high, middle, and low-income settings. We then provide a conceptual framework to inform future global public health research and preparedness across sectors and disciplines and outline key stakeholders recommendations in promoting climate resilient systems and advancing health equity.
The Difficult Balance between Ensuring the Right of Nursing Home Residents to Communication and Their Safety
Authors: Bolcato Matteo , Trabucco Aurilio Marco , Di Mizio Giulio , Piccioni Andrea , Feola Alessandro , Bonsignore Alessandro , Tettamanti Camilla , Ciliberti Rosagemma , Rodriguez Daniele , Aprile Anna
Published: March 2021

The COVID-19 epidemic has had a profound impact on healthcare systems worldwide. The number of infections in nursing homes for the elderly particularly is significantly high, with a high mortality rate as a result. In order to contain infection risks for both residents and employees of such facilities, the Italian government passed emergency legislation during the initial stages of the pandemic to restrict outside visitor access. On 30 November 2020, the Italian President of the Council of Ministers issued a new decree recognizing the social and emotional value of visits to patients from family and friends. In addition, it indicated prevention measures for the purposes of containing the infection risk within nursing homes for the elderly. This article comments on these new legislative provisions from the medicolegal perspective, providing indications that can be used in clinical practice.
Validity and Reliability of the Malay Versions of Bloating Severity (BSQ-M) and Quality of Life (BLQoL-M) Questionnaires
Authors: Mahd-Ab.lah Nurzulaikha , Kueh Yee Cheng , Kuan Garry , Yahaya Fatan Hamamah , Wong Mung Seong , Abd Samat Nor Aslina , Hamid Nurhazwani , Mohamad Nor Nurhayati , Whitehead William E. , Thiwan Syed Ismail , Lee Yeong Yeh
Published: March 2021

Abdominal bloating (AB) is a prevalent and bothersome symptom, but there are no specific measures for severity and quality of life (QoL) other than the Bloating Severity Questionnaire (BSQ) and Bloating Quality of Life (BLQoL). We aimed to translate the BSQ and BLQoL into the Malay language and to validate them using exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) approaches. The 12-item BSQ has two components, seven-item severity in general (SevGen) and five-item severity in the past 24 h (Sev24), and BLQOL has five items. Translation to the Malay language (BSQ-M and BLQoL-M) was performed using standard forward and backward processes. EFA followed by CFA were performed in participants with AB due to functional bowel disorders, with the purpose of examining the validity and reliability of the questionnaires translated into Malay. After EFA with 152 participants, all the items of BSQ-M remained in the model. Total variance extracted was 53.26% for BSQ-M and 58.79% for BLQoL-M. The internal consistency based on Cronbach’s alpha values was 0.52 for SevGen, 0.86 for Sev24, and 0.81 for BLQoL-M. After performing CFA with another 323 participants, the final measurement model for BSQ-M and BLQoL-M fit the data well in terms of several fit indices (BSQ-M: root mean square error of approximation (RMSEA) = 0.050, Comparative Fit Index (CFI) = 0.966, Tucker–Lewis Fit Index (TLI) = 0.956, and standardized root mean squared residual (SRMR) = 0.051; BLQoL-M: RMSEA = 0.071, CFI = 0.985, TLI = 0.962, SRMR = 0.021). The composite reliability for BSQ-M and BLQoL-M were satisfactory (SevGen = 0.83, Sev24 = 0.89, BLQoL = 0.80). The intraclass correlation (ICC) results showed excellent stability for BSQ-M and BLQoL-M, ranging from 0.74 to 0.93. The Malay language versions of BSQ-M and BLQoL-M are valid and reliable instruments for measuring the severity and QoL of AB for the Asian population with functional bowel disorders.
Psychological Distress among Italian University Students Compared to General Workers during the COVID-19 Pandemic
Authors: Romeo Annunziata , Benfante Agata , Castelli Lorys , Di Tella Marialaura
Published: March 2021

The COVID-19 pandemic induced numerous changes in the daily life of every individual, with important social, economic, and psychological consequences. Particularly, the psychological impact encountered among students might be affected by social isolation, concern for personal health and for the health of family members and friends, and uncertainty about academic progress. The present study aimed to investigate the psychological impact of the COVID-19 outbreak on Italian university students compared to general workers. The responses of 956 participants (478 university students and 478 workers) were included in the final dataset. Participants were asked to provide sociodemographic and occupation-related information, and to complete: (1) COVID-19-related questions; (2) health-related visual analogue scales; (3) State-Trait Anxiety Inventory-Form Y1 (STAI Y1); and (4) the Beck Depression Inventory (BDI-II). Results of comparisons between university students and general workers revealed that the former reported higher levels of anxiety and depressive symptoms. Furthermore, regression analyses showed that in university students, gender, health evaluation, and health concern and gender, educational level, and health evaluation significantly predicted anxiety and depressive symptoms, respectively. Taken together these findings suggest that specific factors could predispose University students to a high risk of developing mental health symptoms as a consequence of the COVID-19 pandemic.
Prenatal Sociodemographic Factors Predicting Maltreatment of Children up to 3 Years Old: A Prospective Cohort Study Using Administrative Data in Japan
Authors: Isumi Aya , Takahashi Kunihiko , Fujiwara Takeo
Published: March 2021

Identifying risk factors from pregnancy is essential for preventing child maltreatment. However, few studies have explored prenatal risk factors assessed at pregnancy registration. This study aimed to identify prenatal risk factors for child maltreatment during the first three years of life using population-level survey data from pregnancy notification forms. This prospective cohort study targeted all mothers and their infants enrolled for a 3- to 4-month-old health check between October 2013 and February 2014 in five municipalities in Aichi Prefecture, Japan, and followed them until the child turned 3 years old. Administrative records of registration with Regional Councils for Children Requiring Care (RCCRC), which is suggestive of child maltreatment cases, were linked with survey data from pregnancy notification forms registered at municipalities (n = 893). Exact logistic regression was used for analysis. A total of 11 children (1.2%) were registered with RCCRC by 3 years of age. Unmarried marital status, history of artificial abortion, and smoking during pregnancy were significantly associated with child maltreatment. Prenatal risk scores calculated as the sum of these prenatal risk factors, ranging from 0 to 7, showed high predictive power (area under receiver operating characteristic curve 0.805; 95% confidence interval (CI), 0.660–0.950) at a cut-off score of 2 (sensitivity = 72.7%, specificity = 83.2%). These findings suggest that variables from pregnancy notification forms may be predictors of the risk for child maltreatment by the age of three.
Joint Flexibility and Isometric Strength Parameters Are Not Relevant Determinants for Countermovement Jump Performance
Authors: Konrad Andreas , Reiner Marina Maren , Bernsteiner Daniel , Glashüttner Christoph , Thaller Sigrid , Tilp Markus
Published: March 2021

Vertical jumps are of great importance as a performance predictor for many types of sports that require speed and agility. However, to date, it is not clear if flexibility and/or the strength of the different leg muscles are determinants for countermovement jump (CMJ) performance. Therefore, the purpose of this study was to relate isometric maximum voluntary contraction (MVC) torque and the flexibility of various muscle groups of the lower body with CMJ performance. Thirty-six healthy male volunteers participated in this study. The participants performed MVCs of the knee extensors, knee flexors, and plantar flexors on a dynamometer. Moreover, range of motion of the hip flexors and plantar flexors was assessed with 3D motion capture, and the range of motion of the knee flexors (hamstrings) was assessed with a Sit n’ Reach® box. CMJs were assessed with a force platform. The correlation analysis revealed a significant moderate correlation of CMJ height with the flexibility of the hip flexors (rP = −0.39) and plantar flexors (rP = 0.47), but not the knee flexors. Moreover, we found that absolute MVC values are not related to CMJ height. However, we did find that knee extensor MVC relative to body mass is significantly related to CMJ height (rP = 0.33) with a moderate magnitude. Although we found significant correlations, the magnitudes of correlations vary between trivial and large according to a 90% confidence interval. Thus, this indicates that range of motion or strength of the assessed leg muscles can explain CMJ performance only to a limited extent.
Physical Activity during the First COVID-19-Related Lockdown in Italy
Authors: Füzéki Eszter , Schröder Jan , Carraro Nicolò , Merlo Laura , Reer Rüdiger , Groneberg David A. , Banzer Winfried
Published: March 2021

The spread of the COVID-19 virus was met by a strict lockdown in many countries around the world, with the closure of all physical activity (PA) facilities and limitations on moving around freely. The aim of the present online survey was to assess the effect of lockdown on physical activity in Italy. Physical activity was assessed using the European Health Interview Survey questionnaire. A total of 1500 datasets were analyzed. Differences between conditions were tested with a chi2-based (χ2) test for categorical variables, and with the Student’s t-test for paired data. A fixed effects binary logistic regression analysis was conducted to identify relevant predictor variables to explain the compliance with World Health Organisation (WHO) recommendations. We found a substantial decline in all physical activity measures. Mean differences in walking and cycling metabolic equivalent of task minutes per week (METmin/week), respectively, were 344.4 (95% confidence interval (95% CI): 306.6–382.2; p < 0.001) and 148.5 (95% CI: 123.6–173.5; p < 0.001). Time spent in leisure time decreased from 160.8 to 112.6 min/week (mean difference 48.2; 95% CI: 40.4–56.0; p < 0.001). Compliance with WHO recommendations decreased from 34.9% to 24.6% (chi2 (1, 3000) = 38.306, p < 0.001, V = 0.11). Logistic regression showed a reduced chance (OR 0.640, 95% CI: 0.484–0.845; p = 0.001) to comply with WHO PA recommendations under lockdown conditions. Measures to promote physical activity should be intensified to limit detrimental health effects.
Environmental Exposure of Wild Carnivores to Zoonotic Pathogens: Leptospira Infection in the First Free Living Wolf (Canis lupus Linnaeus, 1758) Found Dead in the Friuli Venezia Giulia Region
Authors: Bregoli Marco , Pesaro Stefano , Ustulin Martina , Vio Denis , Beraldo Paola , Galeotti Marco , Cocchi Monia , Lucchese Laura , Bertasio Cristina , Boniotti Maria Beatrice , Lapini Luca , Natale Alda
Published: March 2021

Leptospirosis is a worldwide-spread zoonosis causing disease and death in dogs and in humans. A Leptospiral infection has been recorded in several wild carnivore species in Europe, but tissue pathological changes were not commonly described. The Grey wolf (Canis lupus) has been expanding its distribution range in north-eastern Italy during the last decade. A young wolf, representing the first individual handled in the region, was found road-killed and then submitted to necropsy. Pathological changes included erosive lesions of gingival mucosa, mild liver enlargement, and multifocal degenerative-necrotic areas along with hyperemic reactive lesions; multifocal interstitial nephritis and multifocal lung hemorrhages were observed. A Polymerase Chain Reaction (PCR) able to detect pathogenic species of Leptospira performed on a kidney sample was positive. Serological reactions for serogroup Gryppotyphosa (1:6400), Pomona (1:800), and Icterohaemorrhagiae (1:200) were evidenced by MAT. Genotyping by Multilocus Sequence Typing (MLST) performed on detected Leptospira characterized it as belonging to Sequence Type (ST) 117, which refers to L. kirschneri, serogroup Pomona, serovar Mozdok. Regardless of the role of Leptospira infection as an eventual predisposing factor to the road killing of this wolf, to the best of the authors’ knowledge, this is the first report of Leptospira-induced pathology in a wolf in Europe. Surveys on Leptospira infection in free-ranging wildlife species should be pursued in order to achieve further epidemiological knowledge on the circulation of the Leptospira strain.
Feasibility, Acceptability, and Outcomes of a Yoga-Based Meditation Intervention for Hospice Professionals to Combat Burnout
Authors: Heeter Carrie , Allbritton Marcel , Lehto Rebecca , Miller Patrick , McDaniel Patricia , Paletta Michael
Published: March 2021

(1) Background. This research examined the feasibility, acceptability and outcomes of delivering a 6-week yoga-based meditation intervention to clinical teams of hospice professionals (HPs) at a large non-profit hospice organization. The intervention was designed to increase mind-body integration and combat burnout. This article was written for different audiences, including research scientists who study interoception, burnout, meditation, or yoga, designers of meditation interventions, and hospice organizations looking for ways to mitigate HP burnout. (2) Methods. The intervention was launched within clinical teams, beginning with a half-hour online introduction to the program and exposure to the week 1 meditation at each team’s monthly all-staff meeting. Throughout the program, HPs could access the meditations on their own via their workplace computers, tablets, and smartphones. Online pre- and post-intervention surveys were submitted by 151 HPs, 76 of whom were exposed to the intervention and completed both surveys. The surveys assessed burnout using the Professional Fulfillment Index and mind-body integration using the Multidimensional Assessment of Interoceptive Awareness scales. (3) Results. Two-thirds of HPs who were present at a staff meeting where the program was introduced went on to do a meditation on their own at least once. Half of HPs expressed a desire to continue with access to the meditations after the 6-week program ended. Due to COVID-19 work from home restrictions, three-fourth of HPs did a meditation at home, 29% in a car between patient visits (not while driving), and 23% at the office. Higher interoceptive awareness was significantly related to lower burnout, particularly lower work exhaustion. Meditation frequency was significantly related to higher interoceptive awareness but not to burnout. Interpersonal disengagement was rare and temporary. (4) Conclusions. Findings showed that the yoga-based meditation intervention was feasible and acceptable and associated with higher interoceptive awareness. The results point to a role for interoceptive awareness in reducing the risk for burnout.
Electromyographic Evaluation of the Shoulder Muscle after a Fatiguing Isokinetic Protocol in Recreational Overhead Athletes
Authors: Klich Sebastian , Kawczyński Adam , Pietraszewski Bogdan , Zago Matteo , Chen Aiguo , Smoter Małgorzata , Hassanlouei Hamidollah , Lovecchio Nicola
Published: March 2021

The goal of our study was to examine the muscle activity of the shoulder girdle after isokinetic fatigue, which may simulate muscle activities commonly occurring during specific sport-related activities in recreational overhead asymptomatic athletes. We hypothesized that exercise-induced fatigue, reported after isokinetic protocols, may cause a decrease in the median frequency (MF) of the upper trapezius (UT), infraspinatus (IS), and deltoid muscles. Twenty-four male overhead volleyball (n = 8), handball (n = 8), and tennis (n = 8) athletes participated in this study. All subjects were without shoulder injury history. The surface electromyography (SEMG) was collected on the right (dominant) side of the shoulder girdle muscles in the following order: UT, IS and anterior (DA), and posterior deltoideus (DP). The fatigue protocol consisted of three sets of 32 maximum isokinetic concentric contractions while performing shoulder internal and external rotation at an isokinetic speed of 120 o/s. The resultant difference in median frequency (ΔMF) values consistently dropped after the fatiguing tasks across all recorded muscles, in terms of the initial MF (MFINI = 65.1 ± 1.1 Hz) and final MF (MFFIN = 57.9 ± 0.9 Hz), and the main effect of time was significant (F(1,22) = 43.15, p < 0.001). MF values decreased mostly for IS (ΔMFIS = −9.9 ± 1.6 Hz) and DP (ΔMFPD = −9.5 ± 1.9 Hz) muscles, while DA and UT showed smaller changes (ΔMFDA = −6.9 ± 1.5 Hz) and (ΔMFUT = −3.2 ± 1.3 Hz). The results of our study show a meaningful contribution in determining increased fatigue of the shoulder girdle muscles during repeated isokinetic internal-external rotation protocols. We have also demonstrated a significant decrease in MF in all examined muscles, especially IS and DA.
Mining Complex Genetic Patterns Conferring Multiple Sclerosis Risk
Authors: Briggs Farren B. S. , Sept Corriene
Published: March 2021

(1) Background: Complex genetic relationships, including gene-gene (G × G; epistasis), gene(n), and gene-environment (G × E) interactions, explain a substantial portion of the heritability in multiple sclerosis (MS). Machine learning and data mining methods are promising approaches for uncovering higher order genetic relationships, but their use in MS have been limited. (2) Methods: Association rule mining (ARM), a combinatorial rule-based machine learning algorithm, was applied to genetic data for non-Latinx MS cases (n = 207) and controls (n = 179). The objective was to identify patterns (rules) amongst the known MS risk variants, including HLA-DRB1*15:01 presence, HLA-A*02:01 absence, and 194 of the 200 common autosomal variants. Probabilistic measures (confidence and support) were used to mine rules. (3) Results: 114 rules met minimum requirements of 80% confidence and 5% support. The top ranking rule by confidence consisted of HLA-DRB1*15:01, SLC30A7-rs56678847 and AC093277.1-rs6880809; carriers of these variants had a significantly greater risk for MS (odds ratio = 20.2, 95% CI: 8.5, 37.5; p = 4 × 10−9). Several variants were shared across rules, the most common was INTS8-rs78727559, which was in 32.5% of rules. (4) Conclusions: In summary, we demonstrate evidence that specific combinations of MS risk variants disproportionately confer elevated risk by applying a robust analytical framework to a modestly sized study population.
Mental Health in the Era of the Second Wave of SARS-CoV-2: A Cross-Sectional Study Based on an Online Survey among Online Respondents in Poland
Authors: Babicki Mateusz , Szewczykowska Ilona , Mastalerz-Migas Agnieszka
Published: March 2021

The main objective of this study was to assess mental health during the COVID-19 second wave. The study was conducted using a proprietary questionnaire that had been provided via the Internet to online respondents in Poland. The questionnaire questions included a socio-geographic assessment, proprietary questions assessing the respondents’ current approach to the COVID-19 pandemic, as well as a standardised psychometric tool—GHQ-28. The study involved 2155 respondents, 99.8% of whom gave their consent for the participation in the study. A mean GHQ score was 29.25 ± 14.94 points. The criterion for minor mental disorders (≥24 points) was met by 1272 (59.2%) respondents. In overall interpretation as well as in each of GHQ-28 subscales, women obtained significantly higher scores than men (p < 0.001). The restriction on earning opportunities during the COVID-19 pandemic is significantly associated with the feeling of anxiety/insomnia severity among the respondents (9.96 vs. 8.82 points; p < 0.001). The COVID-19 pandemic, although it has already been experienced for nearly a year, has had a significant association with the general mental health of the respondents in Poland. There is a strong need to implement special programs that offer psychological support in the era of the COVID-19 pandemic, especially for those who had direct experience with COVID-19 infection.
Current Advancements in Noninvasive Profiling of the Embryo Culture Media Secretome
Authors: Zmuidinaite Raminta , Sharara Fady I. , Iles Ray K.
Published: March 2021

There have been over 8 million babies born through in vitro fertilization (IVF) and this number continues to grow. There is a global trend to perform elective single embryo transfers, avoiding risks associated with multiple pregnancies. It is therefore important to understand where current research of noninvasive testing for embryos stands, and what are the most promising techniques currently used. Furthermore, it is important to identify the potential to translate research and development into clinically applicable methods that ultimately improve live birth and reduce time to pregnancy. The current focus in the field of human reproductive medicine is to develop a more rapid, quantitative, and noninvasive test. Some of the most promising fields of research for noninvasive assays comprise cell-free DNA analysis, microscopy techniques coupled with artificial intelligence (AI) and omics analysis of the spent blastocyst media. High-throughput proteomics and metabolomics technologies are valuable tools for noninvasive embryo analysis. The biggest advantages of such technology are that it can differentiate between the embryos that appear morphologically identical and has the potential to identify the ploidy status noninvasively prior to transfer in a fresh cycle or before vitrification for a later frozen embryo transfer.
Characterization of Estrogenic Activity and Site-Specific Accumulation of Bisphenol-A in Epididymal Fat Pad: Interfering Effects on the Endocannabinoid System and Temporal Progression of Germ Cells
Authors: Chioccarelli Teresa , Migliaccio Marina , Suglia Antonio , Manfrevola Francesco , Porreca Veronica , Diano Nadia , Errico Sonia , Fasano Silvia , Cobellis Gilda
Published: March 2021

The objective of this work has been to characterize the estrogenic activity of bisphenol-A (BPA) and the adverse effects on the endocannabinoid system (ECS) in modulating germ cell progression. Male offspring exposed to BPA during the foetal-perinatal period at doses below the no-observed-adverse-effect-level were used to investigate the exposure effects in adulthood. Results showed that BPA accumulates specifically in epididymal fat rather than in abdominal fat and targets testicular expression of 3β-hydroxysteroid dehydrogenase and cytochrome P450 aromatase, thus promoting sustained increase of estrogens and a decrease of testosterone. The exposure to BPA affects the expression levels of some ECS components, namely type-1 (CB1) and type-2 cannabinoid (CB2) receptor and monoacylglycerol-lipase (MAGL). Furthermore, it affects the temporal progression of germ cells reported to be responsive to ECS and promotes epithelial germ cell exfoliation. In particular, it increases the germ cell content (i.e., spermatogonia while reducing spermatocytes and spermatids), accelerates progression of spermatocytes and spermatids, promotes epithelial detachment of round and condensed spermatids and interferes with expression of cell–cell junction genes (i.e., zonula occcludens protein-1, vimentin and β-catenin). Altogether, our study provides evidence that early exposure to BPA produces in adulthood sustained and site-specific BPA accumulation in epididymal fat, becoming a risk factor for the reproductive endocrine pathways associated to ECS.
The Role of White Matter Dysfunction and Leukoencephalopathy/Leukodystrophy Genes in the Aetiology of Frontotemporal Dementias: Implications for Novel Approaches to Therapeutics
Authors: Lok Hiu Chuen , Kwok John B.
Published: March 2021

Frontotemporal dementia (FTD) is a common cause of presenile dementia and is characterized by behavioural and/or language changes and progressive cognitive deficits. Genetics is an important component in the aetiology of FTD, with positive family history of dementia reported for 40% of cases. This review synthesizes current knowledge of the known major FTD genes, including C9orf72 (chromosome 9 open reading frame 72), MAPT (microtubule-associated protein tau) and GRN (granulin), and their impact on neuronal and glial pathology. Further, evidence for white matter dysfunction in the aetiology of FTD and the clinical, neuroimaging and genetic overlap between FTD and leukodystrophy/leukoencephalopathy are discussed. The review highlights the role of common variants and mutations in genes such as CSF1R (colony-stimulating factor 1 receptor), CYP27A1 (cytochrome P450 family 27 subfamily A member 1), TREM2 (triggering receptor expressed on myeloid cells 2) and TMEM106B (transmembrane protein 106B) that play an integral role in microglia and oligodendrocyte function. Finally, pharmacological and non-pharmacological approaches for enhancing remyelination are discussed in terms of future treatments of FTD.
Product Owner’s Journey to SAFe®—Role Changes in Scaled Agile Framework®
Authors: Remta Daniel , Buchalcevova Alena
Published: March 2021

As agile software development methods are spreading through the industry, they are no longer sufficient in their original design. With the increasing adoption by various types and sizes of organizations, these methods are scaled and tailored. The most popular framework for scaling Agile is the Scaled Agile Framework®, registered trademark of Scaled Agile, Inc. Boulder, USA. Some roles originating from the agile methods, such as Product Owner, are part of the framework and are impacted by scaling and tailoring. A Product Owner role is critical to the success of projects in agile environments. This paper aims to describe and discuss the evolution of and changes in Product Owner activities since Agile started to spread in the industry until the current concept of the Product Owner role in the Scaled Agile Framework. By identifying the activities typical of Product Owners outside of the Scaled Agile Framework context and mapping these activities to the Product Owner role description in Scaled Agile Framework, the changes in Product Owner role with respect to time and role specifics in the Scaled Agile Framework are revealed. It was identified that some of the activities previously described for Product Owner are distributed between various roles in the Scaled Agile Framework. In fact, the Product Owner loses the real product ownership in Scaled Agile Framework. The loss of ownership seems connected with the fact that, in the large environments that the Scaled Agile Framework is designed for, it is impossible to cover all required activities by one role using the hierarchical structures with a top-down approach in the Scaled Agile Framework.
Concentration-Dependent Feeding Deterrence to 20-Hydroxyecdysone for Three Subterranean Termite Species (Blattodea: Rhinotermitidae)
Authors: Carnohan Lucas , Lee Sang-Bin , Su Nan-Yao
Published: March 2021

Effective active ingredients in toxicant bait formulations must be non-deterrent to insect feeding behavior at lethal concentrations. This study evaluated feeding deterrence for Coptotermes formosanus Shiraki, C. gestroi (Wasmann), and Reticulitermes flavipes (Kollar) when provided access to cellulose impregnated with various concentrations of the insect molting hormone, 20-hydroxyecdysone (20E). Termites were exposed to 20E concentrations of 200, 500, 1000 and 2000 ppm and to noviflumuron at 5000 ppm in a 24 h choice-test, and the mass of substrate consumption from treated and untreated media pads was compared for each treatment. 20E feeding deterrence was detected at 500, 1000 and 2000 ppm for C. gestroi, and at 2000 ppm for C. formosanus. No significant differences in consumption of treated and untreated substrate was detected at any concentration for R. flavipes. Potential methods for reducing deterrence are discussed.