bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychological Correlates of Ghosting and Breadcrumbing Experiences: A Preliminary Study among Adults
Authors: Navarro Raúl , Larrañaga Elisa , Yubero Santiago , Víllora Beatriz
Published: February 2020

The present study aimed to examine differences in three psychological constructs (satisfaction with life, loneliness, and helplessness) among adults experiencing ghosting and breadcrumbing. A sample of 626 adults (303 males and 323 females), aged from 18 to 40 years, completed an online survey asking to indicate whether someone they considered a dating partner had ghosted or breadcrumbed them in the last year and to complete three different scales regarding satisfaction with life, loneliness, and helplessness. The results showed than those participants who had indicated experiencing breadcrumbing or the combined forms (both breadcrumbing and ghosting) reported less satisfaction with life, and more helplessness and self-perceived loneliness. The results from the regression models showed that suffering breadcrumbing would significantly increase the likelihood of experiencing less satisfaction with life, and of having more feelings of loneliness and helplessness. However, no significant relation was found between ghosting and any of the examined psychological correlates.
Cardiac Autonomic Modulation during on-Call Duty under Working Hours Restriction
Authors: Chien Jien-Wen , Chen Chung-Yen , Lin Sheng-Hsuan , Lin Shih-Wen , Lin Yu-Hsuan
Published: February 2020

Background: Medical residency is a time of high stress and long working hours, which increase the risk of cardiovascular disease. This study aimed to investigate the autonomic modulation of resident physicians throughout the on-call duty cycle. Methods: Spectral analysis of heart rate variability (HRV) was used to compute cardiac parasympathetic modulation (high-frequency power, HF) and cardiac sympathetic modulation (normalized low-frequency power, LF%, and the ratio of LF and HF, LF/HF) of 18 residents for a consecutive 4-day cycle. Results: Male residents show reduced cardiac sympathetic modulation (i.e., higher LF/HF and LF%) than the female interns. Medical residents’ cardiac parasympathetic modulation (i.e., HF) significantly increased on the first and the second post-call day compared with the pre-call day. In contrast, LF% was significantly decreased on the first and the second post-call day compared with the pre-call day. Similarly, LF/HF was significantly decreased on the second post-call day compared with the pre-call day. LF/HF significantly decreased on the first post-call day and on the second post-call day from on-call duty. Conclusion: The guideline that limits workweeks to 80 h and shifts to 28 h resulted in reduced sympathetic modulation and increased parasympathetic modulation during the two days following on-call duty.
Performance of Exoelectrogenic Bacteria Used in Microbial Desalination Cell Technology
Authors: Guang Li , Koomson Desmond Ato , Jingyu Huang , Ewusi-Mensah David , Miwornunyuie Nicholas
Published: February 2020

The tri-functional purpose of Microbial Desalination Cell (MDC) has shown a great promise in our current scarcity of water, an increase in water pollution and the high cost of electricity production. As a biological system, the baseline force that drives its performance is the presence of exoelectrogens in the anode chamber. Their presence in the anodic chamber of MDC systems enables the treatment of water, desalination of seawater, and the production of electrical energy. This study reviews the characteristics of exoelectrogens, as a driving force in MDC and examines factors which influence their growth and the performance efficiency of MDC systems. It also addresses the efficiency of mixed cultures with certain predominant species as compared to pure cultures used in MDC systems. Furthermore, the study suggests the need to genetically modify certain predominant strains in mixed cultures to enhance their performance in COD removal, desalination and power output and the integration of MDC with other technologies for cost-effective processes.
Child Nutrition Trends Over the Past Two Decades and Challenges for Achieving Nutrition SDGs and National Targets in China
Authors: Yang Bo , Huang Xin , Liu Qin , Tang Shenglan , Story Mary , Chen Yiwen , Zhou Maigeng
Published: February 2020

Background: The objectives of the current study were to identify the trends in child nutrition, the gaps in achieving child nutrition-related goals, and implications for program and policy options for the Chinese government. Methods: Eight child nutrition-related indicators from the Sustainable Development Goals (SDGs) and China’s national nutrition plans, and two datasets, Global Burden of Disease 2016 and Chinese National Nutrition and Health Surveillance, were used in our analysis. Results: Over the past 26 years, the prevalence of stunting, wasting, and underweight for children under 5 years was reduced by 58.7%, 53.4%, and 69.2%, respectively. Overweight for children aged 1–4 years increased 88.9% and obesity increased 2.14 times. Exclusive breastfeeding of newborns (7–28 days) was stable, at about 30%. We estimated child wasting would be 3.0% lower than the target of 5.0% based on predictive values for meeting the SDGs in 2025. The number of stunted children under five years would be reduced by 39.7%, while overweight would increase 2.2% throughout China. Conclusion: These results highlight the urgent need for targeted policies and interventions to reduce child stunting and overweight and increase exclusive breastfeeding to improve child health and meet the SDG targets and China’s national goals.
The Potential of Ultraviolet Radiation Meters in Secondary Schools as a Sun Protection Intervention Mechanism for Adolescents
Authors: Pettigrew Simone , Parnell Ashleigh , Strickland Mark , Neale Rachel , Lucas Robyn
Published: February 2020

The aim of this pilot study was to assess whether the installation of ultraviolet radiation (UVR) meters in secondary schools has the potential to improve adolescents’ sun protection-related knowledge, attitudes, and behaviours and reduce their exposure to UVR during school hours. Data were collected from students at two schools via online pre- and post-intervention surveys, measurement of sunscreen usage, polysulfone UVR exposure badges, and photographs of the schoolyards to assess hat and shade use. Several operational issues limited the quantity and quality of data that could be collected, and findings were mixed. While there were no significant changes in either self-reported or observed sun protection behaviours, there were significant improvements in UVR knowledge among students at the intervention school, and reactions to the meter were highly favourable. Students reported consulting the meter regularly and using it to make decisions about their sun protection behaviours. Overall, the study results offer some support for the use of UVR meters in areas frequented by adolescents and provide insights into the process issues that are likely to need to be addressed when attempting to trial sun protection interventions in schools.
Modified Screen-Printed Potentiometric Sensors based on Man-Tailored Biomimetics for Diquat Herbicide Determination
Authors: Kamel Ayman H. , Amr Abd El-Galil E. , Abdalla Nashwa S. , El-Naggar Mohamed , Al-Omar Mohamed A. , Almehizia Abdulrahman A.
Published: February 2020

Screen-printed platforms integrated with molecularly imprinted polymers (MIP) were fabricated and characterized as potentiometric sensors for diquat (DQ). The synthesized MIP beads were studied as sensory carriers in plasticized poly(vinyl chloride) membranes. The sensors were constructed by using poly(3,4-ethylenedioxythiophene) (PEDOT) as solid-contact material to diminish charge-transfer resistance and water layer potential. Conventional ion-selective electrodes (ISEs) with internal filling solution were used for comparison. The designed electrodes showed near Nernstian slopes of 28.2 ± 0.7 (r² = 0.999) over the concentration range of 1.0 × 10−6–1.0 × 10−2 M with the limit of detection 0.026 µg/mL over the pH range 4.2–9.0. The electrode exhibited good selectivity for diquat cations over a large number of organic and inorganic cations. The sensor was successfully introduced for direct measurement of diquat content in commercial pesticide preparations and different spiked potato samples. The results showed that the proposed electrode has a fast and stable response, good reproducibility, and applicability for direct assessment of diquat content. The proposed potentiometric method is simple and accurate in comparison with the reported HPLC methods. Besides, it is applicable to turbid and colored sample solutions.
The Unique Extended Selection Cohorts Design for the Evaluation of the School-Based Jump-In Intervention on Dietary Habits: A Study Protocol
Authors: Takens Froukje E. , Busch Vincent , Ujčič-Voortman Joanne K. , van Eijsden Manon , Chinapaw Mai J. M.
Published: February 2020

Background: To promote healthy dietary and physical activity behaviour among primary school children, the city of Amsterdam structurally implements the school-based Jump-in intervention in over half of its primary schools. Previously shown to be effective in stimulating physical activity and outside recess play, our study is the first to evaluate Jump-in’s effect on children’s dietary behaviour. Evaluating the effectiveness and implementation process of an intervention in a real-life setting requests an alternative study design. Methods: we chose a mixed-methods, quasi-experimental Extended Selection Cohorts design to evaluate Jump-in’s effectiveness and implementation process. Children and parents from the first ten primary schools that enrolled in the programme in 2016–2017 were invited to participate. The primary outcomes were children’s dietary behaviour and behavioural determinants, assessed by child and parent questionnaires, and photographs of the food and drinks children brought to school. Process indicators, contextual factors and satisfaction with the programme were assessed by interviews with health promotion professionals, school principals, school project coordinators, and teachers; focus group discussions with parents and children; and document analysis. Discussion: Conducting research in a real-life setting is accompanied by methodological challenges. Using an Extended Selection Cohorts design provides a valuable alternative when a Randomized Controlled design is not feasible.
Extracellular Vesicles Mediated Early Embryo–Maternal Interactions
Authors: Bridi Alessandra , Perecin Felipe , Silveira Juliano Coelho da
Published: February 2020

Embryo–maternal crosstalk is an important event that involves many biological processes, which must occur perfectly for pregnancy success. This complex communication starts from the zygote stage within the oviduct and continues in the uterus up to the end of pregnancy. Small extracellular vesicles (EVs) are part of this communication and carry bioactive molecules such as proteins, lipids, mRNA, and miRNA. Small EVs are present in the oviductal and uterine fluid and have important functions during fertilization and early embryonic development. Embryonic cells are able to uptake oviductal and endometrium-derived small EVs. Conversely, embryo-derived EVs might modulate oviductal and uterine function. In this review, our aim is to demonstrate the role of extracellular vesicles modulating embryo–maternal interactions during early pregnancy.
Kidney Inflammation, Injury and Regeneration
Authors: Baer Patrick C. , Koch Benjamin , Geiger Helmut
Published: February 2020

Damage to kidney cells can occur due to a variety of ischemic and toxic insults and leads to inflammation and cell death, which can result in acute kidney injury (AKI) [...]
Transposon Insertion Mutagenesis in Mice for Modeling Human Cancers: Critical Insights Gained and New Opportunities
Authors: Beckmann Pauline J. , Largaespada David A.
Published: February 2020

Transposon mutagenesis has been used to model many types of human cancer in mice, leading to the discovery of novel cancer genes and insights into the mechanism of tumorigenesis. For this review, we identified over twenty types of human cancer that have been modeled in the mouse using Sleeping Beauty and piggyBac transposon insertion mutagenesis. We examine several specific biological insights that have been gained and describe opportunities for continued research. Specifically, we review studies with a focus on understanding metastasis, therapy resistance, and tumor cell of origin. Additionally, we propose further uses of transposon-based models to identify rarely mutated driver genes across many cancers, understand additional mechanisms of drug resistance and metastasis, and define personalized therapies for cancer patients with obesity as a comorbidity.
An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification
Authors: Bissoli Gaetano , Muñoz-Bertomeu Jesús , Bueso Eduardo , Sayas Enric , Vilcara Edgardo A. , Felipo Amelia , Niñoles Regina , Rubio Lourdes , Fernández José A. , Serrano Ramón
Published: February 2020

Intracellular acid stress inhibits plant growth by unknown mechanisms and it occurs in acidic soils and as consequence of other stresses. In order to identify mechanisms of acid toxicity, we screened activation-tagging lines of Arabidopsis thaliana for tolerance to intracellular acidification induced by organic acids. A dominant mutant, sbt4.13-1D, was isolated twice and shown to over-express subtilase SBT4.13, a protease secreted into endoplasmic reticulum. Activity measurements and immuno-detection indicate that the mutant contains less plasma membrane H+-ATPase (PMA) than wild type, explaining the small size, electrical depolarization and decreased cytosolic pH of the mutant but not organic acid tolerance. Addition of acetic acid to wild-type plantlets induces production of ROS (Reactive Oxygen Species) measured by dichlorodihydrofluorescein diacetate. Acid-induced ROS production is greatly decreased in sbt4.13-1D and atrboh-D,F mutants. The latter is deficient in two major NADPH oxidases (NOXs) and is tolerant to organic acids. These results suggest that intracellular acidification activates NOXs and the resulting oxidative stress is important for inhibition of growth. The inhibition of acid-activated NOXs in the sbt4.13-1D mutant compensates inhibition of PMA to increase acid tolerance.
DNA Damage and DNA Damage Response in Chronic Myeloid Leukemia
Authors: Popp Henning D. , Kohl Vanessa , Naumann Nicole , Flach Johanna , Brendel Susanne , Kleiner Helga , Weiss Christel , Seifarth Wolfgang , Saussele Susanne , Hofmann Wolf-Karsten , Fabarius Alice
Published: February 2020

DNA damage and alterations in the DNA damage response (DDR) are critical sources of genetic instability that might be involved in BCR-ABL1 kinase-mediated blastic transformation of chronic myeloid leukemia (CML). Here, increased DNA damage is detected by γH2AX foci analysis in peripheral blood mononuclear cells (PBMCs) of de novo untreated chronic phase (CP)-CML patients (n = 5; 2.5 γH2AX foci per PBMC ± 0.5) and blast phase (BP)-CML patients (n = 3; 4.4 γH2AX foci per PBMC ± 0.7) as well as CP-CML patients with loss of major molecular response (MMR) (n = 5; 1.8 γH2AX foci per PBMC ± 0.4) when compared to DNA damage in PBMC of healthy donors (n = 8; 1.0 γH2AX foci per PBMC ± 0.1) and CP-CML patients in deep molecular response or MMR (n = 26; 1.0 γH2AX foci per PBMC ± 0.1). Progressive activation of erroneous non-homologous end joining (NHEJ) repair mechanisms during blastic transformation in CML is indicated by abundant co-localization of γH2AX/53BP1 foci, while a decline of the DDR is suggested by defective expression of (p-)ATM and (p-)CHK2. In summary, our data provide evidence for the accumulation of DNA damage in the course of CML and suggest ongoing DNA damage, erroneous NHEJ repair mechanisms, and alterations in the DDR as critical mediators of blastic transformation in CML.
Evaluation of Toxic Amyloid β42 Oligomers in Rat Primary Cerebral Cortex Cells and Human iPS-derived Neurons Treated with 10-Me-Aplog-1, a New PKC Activator
Authors: Murakami Kazuma , Yoshimura Mayuko , Nakagawa Shota , Kume Toshiaki , Kondo Takayuki , Inoue Haruhisa , Irie Kazuhiro
Published: February 2020

Amyloid β42 (Aβ42), a causative agent of Alzheimer’s disease (AD), is derived extracellularly from Aβ precursor protein (APP) following the latter’s cleavage by β-secretase, but not α-secretase. Protein kinase Cα (PKCα) activation is known to increase α-secretase activity, thereby suppressing Aβ production. Since Aβ42 oligomer formation causes potent neurotoxicity, APP modulation by PKC ligands is a promising strategy for AD treatment. Although bryostatin-1 (bryo-1) is a leading compound for this strategy, its limited natural availability and the difficulty of its total synthesis impedes further research. To address this limitation, Irie and colleagues have developed a new PKC activator with few side effects, 10-Me-Aplog-1, (1), which decreased Aβ42 in the conditioned medium of rat primary cerebral cortex cells. These results are associated with increased α-secretase but not PKCε-dependent Aβ-degrading enzyme. The amount of neuronal embryonic lethal abnormal vision (nELAV), a known β-secretase stabilizer, was reduced by treatment with 1. Notably, 1 prevented the formation of intracellular toxic oligomers. Furthermore, 1 suppressed toxic oligomerization within human iPS-derived neurons such as bryo-1. Given that 1 was not neurotoxic toward either cell line, these findings suggest that 1 is a potential drug lead for AD therapy.
Dynamics of Salivary Gland AQP5 under Normal and Pathologic Conditions
Authors: Hosoi Kazuo , Yao Chenjuan , Hasegawa Takahiro , Yoshimura Hiroshi , Akamatsu Tetsuya
Published: February 2020

Aquaporin 5 (AQP5) plays an important role in the salivary gland function. The mRNA and protein for AQP5 are expressed in the acini from embryonic days E13-16 and E17-18, respectively and for entire postnatal days. Ligation-reopening of main excretory duct induces changes in the AQP5 level which would give an insight for mechanism of regeneration/self-duplication of acinar cells. The AQP5 level in the submandibular gland (SMG) decreases by chorda tympani denervation (CTD) via activation autophagosome, suggesting that its level in the SMG under normal condition is maintained by parasympathetic nerve. Isoproterenol (IPR), a β-adrenergic agonist, raised the levels of membrane AQP5 protein and its mRNA in the parotid gland (PG), suggesting coupling of the AQP5 dynamic and amylase secretion-restoration cycle. In the PG, lipopolysaccharide (LPS) is shown to activate mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signalings and potentially downregulate AQP5 expression via cross coupling of activator protein-1 (AP-1) and NF-κB. In most species, Ser-156 and Thr-259 of AQP5 are experimentally phosphorylated, which is enhanced by cAMP analogues and forskolin. cAMP-dependent phosphorylation of AQP5 does not seem to be markedly involved in regulation of its intracellular trafficking but seems to play a role in its constitutive expression and lateral diffusion in the cell membrane. Additionally, Ser-156 phosphorylation may be important for cancer development.
The Impact of Hypoxia on Neutrophil Degranulation and Consequences for the Host
Authors: Lodge Katharine M. , Cowburn Andrew S. , Li Wei , Condliffe Alison M.
Published: February 2020

Neutrophils are key effector cells of innate immunity, rapidly recruited to defend the host against invading pathogens. Neutrophils may kill pathogens intracellularly, following phagocytosis, or extracellularly, by degranulation and the release of neutrophil extracellular traps; all of these microbicidal strategies require the deployment of cytotoxic proteins and proteases, packaged during neutrophil development within cytoplasmic granules. Neutrophils operate in infected and inflamed tissues, which can be profoundly hypoxic. Neutrophilic infiltration of hypoxic tissues characterises a myriad of acute and chronic infectious and inflammatory diseases, and as well as potentially protecting the host from pathogens, neutrophil granule products have been implicated in causing collateral tissue damage in these scenarios. This review discusses the evidence for the enhanced secretion of destructive neutrophil granule contents observed in hypoxic environments and the potential mechanisms for this heightened granule exocytosis, highlighting implications for the host. Understanding the dichotomy of the beneficial and detrimental consequences of neutrophil degranulation in hypoxic environments is crucial to inform potential neutrophil-directed therapeutics in order to limit persistent, excessive, or inappropriate inflammation.
Long-Acting FGF21 Inhibits Retinal Vascular Leakage in In Vivo and In Vitro Models
Authors: Tomita Yohei , Fu Zhongjie , Wang Zhongxiao , Cakir Bertan , Cho Steve S. , Britton William , Sun Ye , Hellström Ann , Talukdar Saswata , Smith Lois E.H.
Published: February 2020

The aim of the current study was to investigate the impact of long-acting fibroblast growth factor 21 (FGF21) on retinal vascular leakage utilizing machine learning and to clarify the mechanism underlying the protection. To assess the effect on retinal vascular leakage, C57BL/6J mice were pre-treated with long-acting FGF21 analog or vehicle (Phosphate Buffered Saline; PBS) intraperitoneally (i.p.) before induction of retinal vascular leakage with intravitreal injection of mouse (m) vascular endothelial growth factor 164 (VEGF164) or PBS control. Five hours after mVEGF164 injection, we retro-orbitally injected Fluorescein isothiocyanate (FITC) -dextran and quantified fluorescence intensity as a readout of vascular leakage, using the Image Analysis Module with a machine learning algorithm. In FGF21- or vehicle-treated primary human retinal microvascular endothelial cells (HRMECs), cell permeability was induced with human (h) VEGF165 and evaluated using FITC-dextran and trans-endothelial electrical resistance (TEER). Western blots for tight junction markers were performed. Retinal vascular leakage in vivo was reduced in the FGF21 versus vehicle- treated mice. In HRMECs in vitro, FGF21 versus vehicle prevented hVEGF-induced increase in cell permeability, identified with FITC-dextran. FGF21 significantly preserved TEER compared to hVEGF. Taken together, FGF21 regulates permeability through tight junctions; in particular, FGF21 increases Claudin-1 protein levels in hVEGF-induced HRMECs. Long-acting FGF21 may help reduce retinal vascular leakage in retinal disorders and machine learning assessment can help to standardize vascular leakage quantification.
Calmodulin and Calmodulin Binding Proteins in Dictyostelium: A Primer
Authors: O’Day Danton H. , Taylor Ryan J. , Myre Michael A.
Published: February 2020

Dictyostelium discoideum is gaining increasing attention as a model organism for the study of calcium binding and calmodulin function in basic biological events as well as human diseases. After a short overview of calcium-binding proteins, the structure of Dictyostelium calmodulin and the conformational changes effected by calcium ion binding to its four EF hands are compared to its human counterpart, emphasizing the highly conserved nature of this central regulatory protein. The calcium-dependent and -independent motifs involved in calmodulin binding to target proteins are discussed with examples of the diversity of calmodulin binding proteins that have been studied in this amoebozoan. The methods used to identify and characterize calmodulin binding proteins is covered followed by the ways Dictyostelium is currently being used as a system to study several neurodegenerative diseases and how it could serve as a model for studying calmodulinopathies such as those associated with specific types of heart arrythmia. Because of its rapid developmental cycles, its genetic tractability, and a richly endowed stock center, Dictyostelium is in a position to become a leader in the field of calmodulin research.
Increased Intra-Abdominal Pressure During Laparoscopic Pneumoperitoneum Enhances Albuminuria via Renal Venous Congestion, Illustrating Pathophysiological Aspects of High Output Preeclampsia
Authors: Dreesen Pauline , Schoutteten Melanie K. , Vande Velde Nele , Kaminski Iris , Heylen Line , De Moor Bart , Malbrain Manu L.N.G. , Gyselaers Wilfried
Published: February 2020

Intra-abdominal hypertension (IAH) causes severe organ dysfunction. Our aim is to evaluate the effect of increased intra-abdominal pressure (IAP) on renal function, hypothesizing that venous congestion may increase proteinuria and fluid retention without endothelial dysfunction. Three urine samples were collected from 32 non-pregnant women undergoing laparoscopic-assisted vaginal hysterectomy (LAVH) and from 10 controls placed in Trendelenburg position for 60 min. Urine sampling was done before (PRE), during or immediately after (PER), and two hours after (POST) the procedure. Urinary albumin, protein and creatinine concentrations were measured in each sample, and ratios were calculated and compared within and between groups. During LAVH, the albumin/creatinine ratio (ACR) increased and persisted POST-procedure, which was not observed in controls. A positive correlation existed between the LAVH duration and the relative change in both ACR and protein/creatinine ratio (PCR) PER- and POST-procedure. Iatrogenic IAH increases urinary ACR and PCR in non-pregnant women via a process of venous congestion. This mechanism might explain the presentation of one specific subtype of late-onset preeclampsia, where no drop of maternal cardiac output is observed.
Soluble ST2 is a Useful Biomarker for Grading Cerebral–Cardiac Syndrome in Patients after Acute Ischemic Stroke
Authors: Sung Pei-Hsun , Lin Hung Sheng , Chen Kuan-Hung , Chiang John Y. , Ko Sheung-Fat , Shao Pei-Lin , Chiang Hsin-Ju , Chu Chi-Hsiang , Li Yi-Chen , Chai Han-Tan , Lin Kun-Chen , Yip Hon-Kan
Published: February 2020

This study tested whether the soluble (s)ST2 is a superb biomarker predictive of moderate to severe cerebral–cardiac syndrome (CCS) (defined as coexisting National Institute of Health Stroke Scale (NIHSS) >8 and left-ventricular ejection fraction (LVEF) <60%) in patients after acute ischemic stroke (IS). Between November 2015 and October 2017, a total of 99 IS patients were prospectively enrolled and categorized into three groups based on NIHSS, i.e., group 1 (NIHSS ≤ 8, n = 66), group 2 (NIHSS = 9-15, n = 14) and group 3 (NIHSS ≥ 16, n = 19), respectively. Blood samples were collected immediately after hospitalization, followed by transthoracic echocardiographic examination. The results showed that the flow cytometric analysis for assessment of inflammatory biomarkers of TLR2+/CD14+cells, TLR4+/CD14+cells, Ly6g+/CD14+cells, and MPO+/CD14+cells, and ELISA assessment for circulatory level of sST2 were significantly higher in groups 2/3 than in group 1 (all p < 0.01). However, these parameters did not show significant differences between groups 2 and 3 (all p > 0.05). The LVEF was significantly lower in group 3 than in group 1 (p < 0.001), but it displayed no difference between groups 1/2 or between groups 2/3. These inflammatory biomarkers ((TLR2+/CD14+cells// TLR4+/CD14+cells// MPO+/CD14+cells) and sST2)) were significantly positively correlated to NIHSS and strongly negatively correlated to LVEF (all p < 0.05). Multivariate analysis demonstrated that both MPO/CD14+cells >20% (p = 0.027) and sST2 ≥ 17,600 (p = 0.004) were significantly and independently predictive of moderate-severe CCS after acute IS. Receiver operating characteristic curve analysis demonstrated that sST2 was the most powerful predictor of CCS with a sensitivity of 0.929 and a specificity of 0.731 (p < 0.001). In conclusion, sST2 is a useful biomarker for prediction of CCS severity in patients after acute IS.
Using the Personalized Advantage Index for Individual Treatment Allocation to Blended Treatment or Treatment as Usual for Depression in Secondary Care
Authors: Friedl Nadine , Krieger Tobias , Chevreul Karine , Hazo Jean Baptiste , Holtzmann Jérôme , Hoogendoorn Mark , Kleiboer Annet , Mathiasen Kim , Urech Antoine , Riper Heleen , Berger Thomas
Published: February 2020

A variety of effective psychotherapies for depression are available, but patients who suffer from depression vary in their treatment response. Combining face-to-face therapies with internet-based elements in the sense of blended treatment is a new approach to treatment for depression. The goal of this study was to answer the following research questions: (1) What are the most important predictors determining optimal treatment allocation to treatment as usual or blended treatment? and (2) Would model-determined treatment allocation using this predictive information and the personalized advantage index (PAI)-approach result in better treatment outcomes? Bayesian model averaging (BMA) was applied to the data of a randomized controlled trial (RCT) comparing the efficacy of treatment as usual and blended treatment in depressive outpatients. Pre-treatment symptomatology and treatment expectancy predicted outcomes irrespective of treatment condition, whereas different prescriptive predictors were found. A PAI of 2.33 PHQ-9 points was found, meaning that patients who would have received the treatment that is optimal for them would have had a post-treatment PHQ-9 score that is two points lower than if they had received the treatment that is suboptimal for them. For 29% of the sample, the PAI was five or greater, which means that a substantial difference between the two treatments was predicted. The use of the PAI approach for clinical practice must be further confirmed in prospective research; the current study supports the identification of specific interventions favorable for specific patients.
A New Site Preparation Protocol That Supports Bone Quality Evaluation and Provides Predictable Implant Insertion Torque
Authors: Velikov Stefan , Susin Cristiano , Heuberger Peter , Irastorza-Landa Ainara
Published: February 2020

When preparing an implant site, clinicians often base their assessment of the bone on subjective tactile and visual cues. This assessment is used to plan the surgical procedure for site preparation, including how many drilling steps will be used. The subjective nature of bone evaluation, consequently, results in poor reproducibility and may lead to under or over preparation of the site. Recently, an unconventional site preparation protocol was developed in which the decision of which instruments to use is dictated by insertion torque of the novel site preparation instrument (OsseoShaper™, Nobel Biocare AB, Gothenburg, Sweden). The aim of this study was to quantify the correlation of the site preparation torques of the new instrument with bone density and maximum implant insertion torques. In vitro and in vivo data showed strong linear correlation between site preparation torque and density and resulted in reliable implant insertion torques, respectively. From our analysis, we conclude that this new instrument and protocol has the potential to eliminate the need for additional intraoperative bone evaluation and may reduce the risk of inadequate preparation of the site due to the ability to serve as a predictor of the final implant insertion torque.
Novel Coronavirus Outbreak in Wuhan, China, 2020: Intense Surveillance Is Vital for Preventing Sustained Transmission in New Locations
Authors: Thompson Robin N.
Published: February 2020

The outbreak of pneumonia originating in Wuhan, China, has generated 24,500 confirmed cases, including 492 deaths, as of 5 February 2020. The virus (2019-nCoV) has spread elsewhere in China and to 24 countries, including South Korea, Thailand, Japan and USA. Fortunately, there has only been limited human-to-human transmission outside of China. Here, we assess the risk of sustained transmission whenever the coronavirus arrives in other countries. Data describing the times from symptom onset to hospitalisation for 47 patients infected early in the current outbreak are used to generate an estimate for the probability that an imported case is followed by sustained human-to-human transmission. Under the assumptions that the imported case is representative of the patients in China, and that the 2019-nCoV is similarly transmissible to the SARS coronavirus, the probability that an imported case is followed by sustained human-to-human transmission is 0.41 (credible interval [0.27, 0.55]). However, if the mean time from symptom onset to hospitalisation can be halved by intense surveillance, then the probability that an imported case leads to sustained transmission is only 0.012 (credible interval [0, 0.099]). This emphasises the importance of current surveillance efforts in countries around the world, to ensure that the ongoing outbreak will not become a global pandemic.
Optimization of TIG Welding Parameters Using a Hybrid Nelder Mead-Evolutionary Algorithms Method
Authors: Kshirsagar Rohit , Jones Steve , Lawrence Jonathan , Tabor Jim
Published: February 2020

A number of evolutionary algorithms such as genetic algorithms, simulated annealing, particle swarm optimization, etc., have been used by researchers in order to optimize different manufacturing processes. In many cases these algorithms are either incapable of reaching global minimum or the time and computational effort (function evaluations) required makes the application of these algorithms impractical. However, if the Nelder Mead optimization method is applied to approximate solutions cheaply obtained from these algorithms, the solution can be further refined to obtain near global minimum of a given error function within only a few additional function evaluations. The initial solutions (vertices) required for the application of Nelder-Mead optimization can be obtained through multiple evolutionary algorithms. The results obtained using this hybrid method are better than that obtained from individual algorithms and also show a significant reduction in the computation effort.
Friction-Induced Vibration of a Railway Wheelset-Track System and Its Effect on Rail Corrugation
Authors: Chen Guangxiong
Published: February 2020

In the present study, the effect of the radius of railway curved tracks on the slip of a wheel on a rail is studied. A 3D finite-element model of a wheelset-track system is established when the creep force between the wheel and rail is saturated. The occurrence propensity of the self-excited vibration of the wheelset-track system is predicted. It is concluded that the radius of curved tracks has a strong effect on the slip of wheels on rails. In the tightly curved tracks, the slip of the wheel of the leading wheelset on the rail always occurs. The wheelset-track system has a strong occurrence propensity for unstable vibrations on the tightly curved tracks. The accuracy of the rail corrugation prediction based on the unstable vibrations of wheelset-track systems is determined to be 85–90% or higher.
Misalignment-Induced Micro-Elastohydrodynamic Lubrication in Rotary Lip Seals
Authors: Borras F. Xavier , de Rooij Matthijn B. , Schipper Dik J.
Published: February 2020

In literature the lubrication of rotary lip seals is explained by hydrodynamic action on a microscopic scale. This theory assumes perfect concentricity between the seal and the shaft which in reality seldomly occurs. Focusing on the stern tube seals application, an analysis is performed on the phenomena distorting the axisymmetric operation of rotary lip seals. Radial and angular shaft misalignments together with pressure and temperature gradients have been modelled. The model predictions are validated using a dedicated setup. Additionally, applying the soft-EHL film thickness expressions at the asperity level, an equivalent film thickness along the circumferential direction is estimated. The Reynolds PDE is solved to predict the misalignment-induced hydrodynamic pressure build-up. The film thickness variation derived and accompanying non-uniform contact pressure distribution was shown to be sufficient for hydrodynamic action and, depending on the minimum film thickness, the hydrodynamic pressure build-up can exceed the static contact pressure. Additionally, significant differences were observed between the radial and angular misalignment configurations.
Experimental Study for the Stripping of PTFE Coatings on Al-Mg Substrates Using Dry Abrasive Materials
Authors: Guerrero-Vaca Guillermo , Carrizo-Tejero David , Rodríguez-Alabanda Óscar , Romero Pablo E. , Molero Esther
Published: February 2020

Polytetrafluoroethylene (PTFE) coatings are used in many applications and processing industries. With their use, they wear out and lose properties and must be replaced by new ones if the cost of the element so advises. There are different stripping techniques, but almost all of them are very difficult and require strict environmental controls. It is a challenge to approach the process through efficient and more sustainable techniques. In the present work, we have studied the stripping of PTFE coatings by projection with abrasives (1 step) as an alternative to carbonization + sandblasting procedures (2 steps). For this purpose, different types of abrasives have been selected: brown corundum, white corundum, glass microspheres, plastic particles, and a walnut shell. The tests were performed at pressures from 0.4 to 0.6 MPa on PTFE-coated aluminium substrates of EN AW-5182 H111 alloy. Stripping rates, surface roughness, and substrate hardness have been studied. Scanning electron microscopy (SEM) images of sandblasted specimens have also been obtained. All abrasives improved mechanical and surface properties in one-step vs. two-step processes. The abrasives of plastic and glass microspheres are the most appropriate for the one-step process, which increases the hardness and roughness level Ra in the substrate. Corundum abrasives enable the highest stripping rates.
Effect of Nickel and Titanium on Properties of Fe-Al-Si Alloy Prepared by Mechanical Alloying and Spark Plasma Sintering
Authors: Novák Pavel , Barták Zdeněk , Nová Kateřina , Průša Filip
Published: February 2020

This paper describes the structure and properties of an innovative Fe-Al-Si alloy with a reduced amount of silicon (5 wt. %) in order to avoid excessive brittleness. The alloy was produced by a combination of mechanical alloying and spark plasma sintering. Nickel and titanium were independently tested as the alloying elements for this alloy. It was found that wear resistance, which reached values comparable with tool steels, could be further improved by the addition of nickel. Nickel also improved the high-temperature oxidation behavior, because it lowers the liability of the oxide layers to spallation. Both nickel and titanium increased the hardness of the alloy. Titanium negatively influenced oxidation behavior and wear resistance because of the presence of titanium dioxide in the oxide layer and the brittle silicides that caused chipping wear, respectively.
Progress and Status of Hydrometallurgical and Direct Recycling of Li-Ion Batteries and Beyond
Authors: Larouche François , Tedjar Farouk , Amouzegar Kamyab , Houlachi Georges , Bouchard Patrick , Demopoulos George P. , Zaghib Karim
Published: February 2020

An exponential market growth of Li-ion batteries (LIBs) has been observed in the past 20 years; approximately 670,000 tons of LIBs have been sold in 2017 alone. This trend will continue owing to the growing interest of consumers for electric vehicles, recent engagement of car manufacturers to produce them, recent developments in energy storage facilities, and commitment of governments for the electrification of transportation. Although some limited recycling processes were developed earlier after the commercialization of LIBs, these are inadequate in the context of sustainable development. Therefore, significant efforts have been made to replace the commonly employed pyrometallurgical recycling method with a less detrimental approach, such as hydrometallurgical, in particular sulfate-based leaching, or direct recycling. Sulfate-based leaching is the only large-scale hydrometallurgical method currently used for recycling LIBs and serves as baseline for several pilot or demonstration projects currently under development. Conversely, most project and processes focus only on the recovery of Ni, Co, Mn, and less Li, and are wasting the iron phosphate originating from lithium iron phosphate (LFP) batteries. Although this battery type does not dominate the LIB market, its presence in the waste stream of LIBs causes some technical concerns that affect the profitability of current recycling processes. This review explores the current processes and alternative solutions to pyrometallurgy, including novel selective leaching processes or direct recycling approaches.
Rodlike YMn2O5 Powders Derived from Hydrothermal Process Using Oxygen as Oxidant
Authors: Shi Jun , Wang Jing , He Huifen , Lu Yang , Shi Zhongxiang
Published: February 2020

A facile approach is proposed herein to fabricate YMn2O5 powders with the hydrothermal method with oxygen as an oxidant. The structure and morphology of the as-synthesized YMn2O5 powders were characterized by XRD, SEM, and high-resolution transmission electron microscopy (HRTEM). The results manifested that the main factors that affected the formation of the rod-like YMn2O5 structures were the stirring time, hydrothermal temperature, and hydrothermal time. The oxidation time in the air had a remarkable effect on the final product by oxidizing Mn2+ ions to Mn3+ ions and Mn4+ ions. The obtained YMn2O5 powder was single crystalline and possessed a nanorod morphology, where the growth direction was along the c axis. The possible formation mechanism involved a dissolution–crystallization mechanism. Under the 397 nm excitation, the Mn4+ ions exhibited an intense orange emission at 596 nm. The energy bandgap of YMn2O5 powders was 1.18 eV.
Stability of a Melt Pool during 3D-Printing of an Unsupported Steel Component and Its Influence on Roughness
Authors: Skalon Mateusz , Meier Benjamin , Gruberbauer Andreas , Amancio-Filho Sergio de Traglia , Sommitsch Christof
Published: February 2020

The following work presents the results of an investigation of the cause–effect relationship between the stability of a melt pool and the roughness of an inclined, unsupported steel surface that was 3D-printed using the laser powder bed fusion (PBF-L/M) process. In order to observe the balling effect and decrease in surface quality, the samples were printed with no supporting structures placed on the downskin. The stability of the melt pool was investigated as a function of both the inclination angle and along the length of the melt pool. Single-track cross-sections were described by shape parameters and were compared and used to calculate the forces acting on the melt pool as the downskin was printed. The single-melt track tests were printed to produce a series of samples with increasing inclination angles with respect to the baseplate. The increasing angles enabled us to physically simulate specific solidification conditions during the sample printing process. As the inclination angle of the unsupported surface increased, the melt-pool altered in terms of its size, geometry, contact angles, and maximum length of stability. The balling phenomenon was observed, quantified, and compared using roughness tests; it was influenced by the melt track stability according to its geometry. The research results show that a higher linear energy input may decrease the roughness of unsupported surfaces with low inclination angles, while a lower linear energy input may be more effective with higher inclination angles.
Chitosan-TiO2: A Versatile Hybrid Composite
Authors: Anaya-Esparza Luis Miguel , Ruvalcaba-Gómez José Martín , Maytorena-Verdugo Claudia Ivette , González-Silva Napoleón , Romero-Toledo Rafael , Aguilera-Aguirre Selene , Pérez-Larios Alejandro , Montalvo-González Efigenia
Published: February 2020

In recent years, a strong interest has emerged in hybrid composites and their potential uses, especially in chitosan–titanium dioxide (CS–TiO2) composites, which have interesting technological properties and applications. This review describes the reported advantages and limitations of the functionalization of chitosan by adding TiO2 nanoparticles. Their effects on structural, textural, thermal, optical, mechanical, and vapor barrier properties and their biodegradability are also discussed. Evidence shows that the incorporation of TiO2 onto the CS matrix improves all the above properties in a dose-dependent manner. Nonetheless, the CS–TiO2 composite exhibits great potential applications including antimicrobial activity against bacteria and fungi; UV-barrier properties when it is used for packaging and textile purposes; environmental applications for removal of heavy metal ions and degradation of diverse water pollutants; biomedical applications as a wound-healing material, drug delivery system, or by the development of biosensors. Furthermore, no cytotoxic effects of CS–TiO2 have been reported on different cell lines, which supports their use for food and biomedical applications. Moreover, CS–TiO2 has also been used as an anti-corrosive material. However, the development of suitable protocols for CS–TiO2 composite preparation is mandatory for industrial-scale implementation.
Effect of Grain Size and Surface Roughness on the Normal Coefficient of Restitution of Single Grains
Authors: Sandeep Chitta Sai , Luo Lina , Senetakis Kostas
Published: February 2020

The coefficient of restitution (COR) represents the fraction of pre-collision kinetic energy remained after the collision between two bodies. The COR parameter plays an important role in the discrete numerical analysis of granular flows or the design of protective barriers to reduce flow energy. This work investigated the COR for grain-block type impacts through comprehensive experiments using a custom-built impact loading apparatus. Glass balls of three different sizes were used as grains. The impact experiments were performed on three different types of materials as base blocks, namely brass, granite and rubber. Experiments on the brass block showed a decrease in COR values with increasing grain size. On the contrary, impacts on granite and rubber blocks showed an increase in COR values with increasing grain size. Additionally, the effect of surface roughness on the COR was investigated. It was revealed that the increase in surface roughness of either the grain or the block reduced the COR values due to the increased plastic deformations of surface asperities.
Development of Novel Magnetoliposomes Containing Nickel Ferrite Nanoparticles Covered with Gold for Applications in Thermotherapy
Authors: Rio Irina S. R. , Rodrigues Ana Rita O. , Rodrigues Carolina P. , Almeida Bernardo G. , Pires A. , Pereira A. M. , Araújo J. P. , Castanheira Elisabete M. S. , Coutinho Paulo J. G.
Published: February 2020

Multifunctional nanosystems combining magnetic and plasmonic properties are a promising approach for cancer therapy, allowing magnetic guidance and a local temperature increase. This capability can provide a triggered drug release and synergistic cytotoxic effect in cancer cells. In this work, nickel ferrite/gold nanoparticles were developed, including nickel ferrite magnetic nanoparticles decorated with plasmonic gold nanoparticles and core/shell nanostructures (with a nickel ferrite core and a gold shell). These nanoparticles were covered with a surfactant/lipid bilayer, originating liposome-like structures with diameters below 160 nm. The heating capacity of these systems, upon excitation with light above 600 nm wavelength, was assessed through the emission quenching of rhodamine B located in the lipid layer. The developed nanosystems show promising results for future applications in thermotherapy.