bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Grade Serous Ovarian Cancer: Basic Sciences, Clinical and Therapeutic Standpoints
Authors: Lisio Michael-Antony , Fu Lili , Goyeneche Alicia , Gao Zu-hua , Telleria Carlos
Published: February 2019

Among a litany of malignancies affecting the female reproductive tract, that of the ovary is the most frequently fatal. Moreover, while the steady pace of scientific discovery has fuelled recent ameliorations in the outcomes of many other cancers, the rates of mortality for ovarian cancer have been stagnant since around 1980. Yet despite the grim outlook, progress is being made towards better understanding the fundamental biology of this disease and how its biology in turn influences clinical behaviour. It has long been evident that ovarian cancer is not a unitary disease but rather a multiplicity of distinct malignancies that share a common anatomical site upon presentation. Of these, the high-grade serous subtype predominates in the clinical setting and is responsible for a disproportionate share of the fatalities from all forms of ovarian cancer. This review aims to provide a detailed overview of the clinical-pathological features of ovarian cancer with a particular focus on the high-grade serous subtype. Along with a description of the relevant clinical aspects of this disease, including novel trends in treatment strategies, this text will inform the reader of recent updates to the scientific literature regarding the origin, aetiology and molecular-genetic basis of high-grade serous ovarian cancer (HGSOC).
Effects of Chemotherapy and Radiotherapy on Spermatogenesis: The Role of Testicular Immunology
Authors: Qu Ning , Itoh Masahiro , Sakabe Kou
Published: February 2019

Substantial improvements in cancer treatment have resulted in longer survival and increased quality of life in cancer survivors with minimized long-term toxicity. However, infertility and gonadal dysfunction continue to be recognized as adverse effects of anticancer therapy. In particular, alkylating agents and irradiation induce testicular damage that results in prolonged azoospermia. Although damage to and recovery of spermatogenesis after cancer treatment have been extensively studied, there is little information regarding the role of differences in testicular immunology in cancer treatment-induced male infertility. In this review, we briefly summarize available rodent and human data on immunological differences in chemotherapy or radiotherapy.
Transcriptional Regulation of Acyl-CoA:Glycerol-sn-3-Phosphate Acyltransferases
Authors: Karasawa Ken , Tanigawa Kazunari , Harada Ayako , Yamashita Atsushi
Published: February 2019

Acyl-CoA:glycerol-sn-3-phosphate acyltransferase (GPAT) is an enzyme responsible for the rate-limiting step in the synthesis of glycerophospholipids and triacylglycerol (TAG). The enzymes of mammalian species are classified into four isoforms; GPAT1 and GPAT2 are localized in the mitochondrial outer membrane, whereas GPAT3 and GPAT4 are localized in the endoplasmic reticulum membrane. The activity of each enzyme expressed is associated with physiological and pathological functions. The transcriptional regulation is well known, particularly in GPAT1. GPAT1 mRNA expression is mainly regulated by the binding of the transcriptional factor SREBP-1c to the specific element (the sterol regulatory element) flanking the GPAT1 promoter. The TAG level is controlled by the insulin-induced transcriptional expression of GPAT1, which occupies most of the GPAT activity in the liver. The transcriptional regulation of the other three GPAT isoforms remains undetermined in detail. It is predicted that retinoic acid serves as a transcription factor in the GPAT2 promoter. PPARγ (peroxisome proliferator-activated receptor γ) increases the mRNA expression of GPAT3, which is associated with TAG synthesis in adipose tissues. Although GPAT has been considered to be a key enzyme in the production of TAG, unexpected functions have recently been reported, particularly in GPAT2. It is likely that GPAT2 is associated with tumorigenesis and normal spermatogenesis. In this review, the physiological and pathophysiological roles of the four GPAT isoforms are described, alongside the transcriptional regulation of these enzymes.
The Ameliorative Effect of Silicon on Maize Plants Grown in Mg-Deficient Conditions
Authors: Hosseini Seyed Abdollah , Naseri Rad Sara , Ali Nusrat , Yvin Jean-Claude
Published: February 2019

The importance of magnesium (Mg) for plant growth is well-documented. Silicon (Si)-mediated alleviation of mineral deficiencies has been also reported in a number of plant species; however, there is no report on the relevance of Si nutrition in plants grown in Mg-deficient condition. Therefore, in the present work, an attempt was undertaken to study the role of Si nutrition in maize plants exposed to Mg deficiency. Plants were grown either under low (0.02 mM) or normal (0.5 mM) levels of Mg, with or without Si supplement. We have shown that Mg-deficient plants treated with Si maintained their growth and increased significantly the levels of chlorophyll and soluble sugars compared to those plants which did not receive Si. In addition, the concentrations of hexose-P, and glycolytic intermediate metabolites—mainly organic acids (isocitric and glutamic acids)—were increased in response to Si nutrition, which was associated with an increase in the levels of stress amino acids such as gamma-aminobutyric-acid (GABA), serine and glycine, as well as polyamines putrescine, which overall contributed to Mg stress tolerance. In addition, Si enhanced the levels of phytohormones cytokinin iso-pentenyladenine (IP), iso-pentenyladenine riboside (IPR), jasmonic acid (JA) and its derivate l-isoleucine (JA-ILE). The increase in cytokinin maintained the growth of Mg-deficient plants, while JA and JA-IEA were induced in response to carbohydrates accumulation. Altogether, our study reveals the vital role of Si under Mg deficiency by regulating plant primary metabolite and hormonal changes.
PKCHD: Towards a Probabilistic Knapsack Public-Key Cryptosystem with High Density
Authors: Ping Yuan , Wang Baocang , Tian Shengli , Zhou Jingxian , Ma Hui
Published: February 2019

By introducing an easy knapsack-type problem, a probabilistic knapsack-type public key cryptosystem (PKCHD) is proposed. It uses a Chinese remainder theorem to disguise the easy knapsack sequence. Thence, to recover the trapdoor information, the implicit attacker has to solve at least two hard number-theoretic problems, namely integer factorization and simultaneous Diophantine approximation problems. In PKCHD, the encryption function is nonlinear about the message vector. Under the re-linearization attack model, PKCHD obtains a high density and is secure against the low-density subset sum attacks, and the success probability for an attacker to recover the message vector with a single call to a lattice oracle is negligible. The infeasibilities of other attacks on the proposed PKCHD are also investigated. Meanwhile, it can use the hardest knapsack vector as the public key if its density evaluates the hardness of a knapsack instance. Furthermore, PKCHD only performs quadratic bit operations which confirms the efficiency of encrypting a message and deciphering a given cipher-text.
Genetic Variants in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis: A Bayesian Approach and Systematic Review
Authors: Lee Kwang Seob , Kronbichler Andreas , Pereira Vasconcelos Daniel Fernando , Pereira da Silva Felipe Rodolfo , Ko Younhee , Oh Yeon Su , Eisenhut Michael , Merkel Peter A. , Jayne David , Amos Christopher I. , Siminovitch Katherine A. , Rahmattulla Chinar , Lee Keum Hwa , Shin Jae Il
Published: February 2019

A number of genome-wide association studies (GWASs) and meta-analyses of genetic variants have been performed in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. We reinterpreted previous studies using false-positive report probability (FPRP) and Bayesian false discovery probability (BFDP). This study searched publications in PubMed and Excerpta Medica Database (EMBASE) up to February 2018. Identification of noteworthy associations were analyzed using FPRP and BFDP, and data (i.e., odds ratio (OR), 95% confidence interval (CI), p-value) related to significant associations were separately extracted. Using filtered gene variants, gene ontology (GO) enrichment analysis and protein–protein interaction (PPI) networks were performed. Overall, 241 articles were identified, and 7 were selected for analysis. Single nucleotide polymorphisms (SNPs) discovered by GWASs were shown to be noteworthy, whereas only 27% of significant results from meta-analyses of observational studies were noteworthy. Eighty-five percent of SNPs with borderline p-values (5.0 × 10−8 < p < 0.05) in GWASs were found to be noteworthy. No overlapping SNPs were found between PR3-ANCA and MPO-ANCA vasculitis. GO analysis revealed immune-related GO terms, including “antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class II”, “interferon-gamma-mediated (IFN-γ) signaling pathway”. By using FPRP and BFDP, network analysis of noteworthy genetic variants discovered genetic risk factors associated with the IFN-γ pathway as novel mechanisms potentially implicated in the complex pathogenesis of ANCA-associated vasculitis.
Increased Risk of Multiple Outpatient Surgeries in African-American Carriers of Transthyretin Val122Ile Mutation Is Modulated by Non-Coding Variants
Authors: Polimanti Renato , Nuñez Yaira Z. , Gelernter Joel
Published: February 2019

Background: African-Americans (AAs) have a 3.5% carrier prevalence of Transthyretin (TTR) Val122Ile mutation (rs76992529), which is the genetic cause of a hereditary form of amyloidosis. Methods: We investigated the medical history of Val122Ile carriers and assessed the role of a non-coding variation in 4361 unrelated AAs. Results: We observed that the Ile122 allele was associated with a 6.8-fold increase in the odds of having 10 or more outpatient surgeries (p = 7.81 × 10−5). Stratifying the analysis by sex, the Ile122 allele was associated with a 15.2-fold increase in the odds of having 10 or more outpatient surgeries in men (p = 6.49 × 10−7). A similar sex difference was observed with respect to the association of Val122Ile with musculoskeletal and connective-tissue disorders in an independent cohort of British subjects (n = 361,194, p = 2.47 × 10−13; nmale = 167,020, pmale = 4.02 × 10−24). In Val122Ile African-American carriers, we observed that haplotypes in the upstream region regulating TTR hepatic expression are associated with having 10 or more outpatient surgeries (p = 2.56 × 10−9). Conclusions: TTR Val122Ile showed a large effect with respect to an extreme phenotype identified in medical history that may be related to osteoarthritis, an early sign of the disease. Additionally, the non-coding variation appears to accelerate the negative consequences associated with Val122Ile mutation via TTR expression regulation.
Optimization of Laser Powder Bed Fusion Processing Using a Combination of Melt Pool Modeling and Design of Experiment Approaches: Density Control
Authors: Letenneur Morgan , Kreitcberg Alena , Brailovski Vladimir
Published: February 2019

A simplified analytical model of the laser powder bed fusion (LPBF) process was used to develop a novel density prediction approach that can be adapted for any given powder feedstock and LPBF system. First, calibration coupons were built using IN625, Ti64 and Fe powders and a specific LPBF system. These coupons were manufactured using the predetermined ranges of laser power, scanning speed, hatching space, and layer thickness, and their densities were measured using conventional material characterization techniques. Next, a simplified melt pool model was used to calculate the melt pool dimensions for the selected sets of printing parameters. Both sets of data were then combined to predict the density of printed parts. This approach was additionally validated using the literature data on AlSi10Mg and 316L alloys, thus demonstrating that it can reliably be used to optimize the laser powder bed metal fusion process.
Who Controls the City in the Global Urban Era? Mapping the Dimensions of Urban Geopolitics in Beira City, Mozambique
Authors: Shannon Murtah
Published: February 2019

In recent years, a new era of interventionism has emerged targeting the development of African cities, manifested in ‘fantasy’ urban plans, surging infrastructure investments and global policy agendas. What the implications of this new era will be for specific urban contexts is still poorly understood however. Taking this research agenda as a starting point, this article presents findings of in-depth empirical research on urban development in Beira city, Mozambique, which has recently become the recipient of massive donor investments targeting the built environment. Informed by current debates on urban geopolitics, the article unpacks these mounting global flows while locating them alongside pre-existing struggles over urban space. By doing so three distinct yet inter-related dimensions of urban geopolitics are identified, relating to the workings of the state, so-called ‘informality’ and international donors. Far from representing homogeneous categories, these dimensions each represent contradictory practices and interests which are shaping Beira’s urban trajectory. The article concludes by arguing that the inflow of donor resources has exacerbated pre-existing struggles over urban space while contributing to new contentions in ways which have undermined social equity targets of contemporary global development agendas. In doing so it provides important contributions to current debates on urban development in Africa
A Pricing and Acquisition Strategy for New and Remanufactured High-Technology Products
Authors: Zhou Liangchuan , Gupta Surendra M.
Published: February 2019

New generations of high-technology products are frequently launched before the previous model is sold out. Customers have an incentive to end the use of their old product and purchase a new one with the latest technological innovations. The unsold old models become less attractive, while the supply of remanufactured products from end-of-use products is uncertain in time, quantity, and quality. Other than adjusting the price, upgrading the returning unsold new products may be a source of remedy. This study provides profit maximization models associated with customer choice demand functions based on manufacturer, retailer, and joint supply chain scenarios. Two acquisition strategies are compared: acquire end-of-use products only and collect both end-of-use products and unsold old-style new products. The results reveal that returning the optimal quantity of overstocked new products brings about a greater benefit in all scenarios. Compared to the remanufacturer, the retailer is the optimal undertaker for collecting used products. In addition to this, slow technological development of the new-generation model causes a decrease in profit for the manufacturer. The optimal quantity of new products to be bought back decreases, because both the manufacturer and the retailer prefer to promote unsold outmoded products rather than upgrade the used products.
Effect of Over Rolling Frequency on the Film Formation in Grease Lubricated EHD Contacts under Starved Conditions
Authors: Fischer Dennis , Mues Helko , Jacobs Georg , Stratmann Andreas
Published: February 2019

The service life of rolling bearings is significantly affected by the lubricating film formation in elastohydrodynamic (EHD) contacts. Grease lubricated EHD contacts show a film thickness decay from a characteristic rotational speed, which is referred to as starvation. Thus, the film thickness of grease lubricated contacts differs from that of oil lubricated contacts. However, the base oil properties under fully flooded conditions are commonly assumed to estimate the operating lifetime of grease lubricated bearings, which are usually not fully flooded. Hence, this assumption results in an overestimation of the film thickness for rotational speeds in the range of starvation, which can lead to uncertainties in the bearing design. At high rotational speeds, i.e., high over rolling frequencies, starvation is likely to occur, due to insufficient lubricant supply by replenishment behind the rolling element. Therefore, the focus of this contribution is to investigate the effect of over rolling frequency, and thus replenishment time, on the lubricating film formation in starved, grease lubricated EHD contacts. The film thickness measurements were performed on a ball-on-disc tribometer, which was extended by adding a second ball specimen in front of the measuring ball. By varying the angular distance between the two contacts, the lubricant displacement can be controlled, such that the effect of replenishment time on the film formation can be determined. These investigations should help to establish an advanced understanding of the mechanisms of grease lubrication, and encourage future work with a focus on developing a method to predict the film formation in grease lubricated EHD contacts.
Anti-Inflammatory Activity of β-thymosin Peptide Derived from Pacific Oyster (Crassostrea gigas) on NO and PGE2 Production by Down-Regulating NF-κB in LPS-Induced RAW264.7 Macrophage Cells
Authors: Hwang Dukhyun , Kang Min-jae , Jo Mi Jeong , Seo Yong Bae , Park Nam Gyu , Kim Gun-Do
Published: February 2019

β-thymosin is known for having 43 amino acids, being water-soluble, having a light molecular weight and ubiquitous polypeptide. The biological activities of β-thymosin are diverse and include the promotion of wound healing, reduction of inflammation, differentiation of T cells and inhibition of apoptosis. Our previous studies showed that oyster β-thymosin originated from the mantle of the Pacific oyster, Crassostrea gigas and had antimicrobial activity. In this study, we investigated the anti-inflammatory effects of oyster β-thymosin in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells using human β-thymosin as a control. Oyster β-thymosin inhibited the nitric oxide (NO) production as much as human β-thymosin in LPS-induced RAW264.7 cells. It also showed that oyster β-thymosin suppressed the expression of prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, oyster β-thymosin reduced inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6). Oyster β-thymosin also suppressed the nuclear translocation of phosphorylated nuclear factor-κB (NF-κB) and degradation of inhibitory κB (IκB) in LPS-induced RAW264.7 cells. These results suggest that oyster β-thymosin, which is derived from the mantle of the Pacific oyster, has as much anti-inflammatory effects as human β-thymosin. Additionally, oyster β-thymosin suppressed NO production, PGE2 production and inflammatory cytokines expression via NF-κB in LPS-induced RAW264.7 cells.
Wound Healing Potential of Spirulina Protein on CCD-986sk Cells
Authors: Liu Ping , Choi Jeong-Wook , Lee Min-Kyeong , Choi Youn-Hee , Nam Taek-Jeong
Published: February 2019

Wound healing is a dynamic and complex process. The proliferation and migration of dermal fibroblasts are crucial for wound healing. Recent studies have indicated that the extracts from Spirulina platensis have a positive potential for wound healing. However, its underlying mechanism is not fully understood. Our previous study showed that spirulina crude protein (SPCP) promoted the viability of human dermal fibroblast cell line (CCD-986sk cells). In this study, we further investigated the wound healing effect and corresponding mechanisms of SPCP on CCD-986sk cells. Bromodeoxyuridine (BrdU) assay showed that SPCP promoted the proliferation of CCD-986sk cells. The wound healing assay showed that SPCP promoted the migration of CCD-986sk cells. Furthermore, cell cycle analysis demonstrated that SPCP promoted CCD-986sk cells to enter S and G2/M phases from G0/G1 phase. Western blot results showed that SPCP significantly upregulated the expression of cyclin D1, cyclin E, cyclin-dependent kinase 2 (Cdk2), cyclin-dependent kinase 4 (Cdk4), and cyclin-dependent kinase 6 (Cdk6), as well as inhibited the expression of CDK inhibitors p21 and p27 in CCD-986sk cells. In the meanwhile, SPCP promoted the phosphorylation and activation of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt). However, the phosphorylation of Akt was significantly blocked by PI3K inhibitor (LY294002), which in turn reduced the SPCP-induced proliferation and migration of CCD-986sk cells. Therefore, the results presenting in this study suggested that SPCP can promote the proliferation and migration of CCD-986sk cells; the PI3K/Akt signaling pathway play a positive and important role in these processes.
Antibiofouling Performance by Polyethersulfone Membranes Cast with Oxidized Multiwalled Carbon Nanotubes and Arabic Gum
Authors: Najjar Ahmad , Sabri Souhir , Al-Gaashani Rashad , Atieh Muataz Ali , Kochkodan Viktor
Published: February 2019

Despite extensive research efforts focusing on tackling membrane biofouling, one of the biggest problems associated with membrane technology, there has been little headway in this area. This study presents novel polyethersulfone (PES) membranes synthesized via a phase inversion method at incremental loadings of functionalized oxidized multiwalled carbon nanotubes (OMWCNT) along with 1 wt. % arabic gum (AG). The synthesized OMWCNT were examined using scanning electron microscopy and transmission electron microscopy for morphological changes compared to the commercially obtained carbon nanotubes. Additionally energy-dispersive X-ray spectroscopy was carried out on the raw and OMWCNT materials, indicating an almost 2-fold increase in oxygen content in the latter sample. The cast PES/OMWCNT membranes were extensively characterized, and underwent a series of performance testing using bovine serum albumin solution for fouling tests and model Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) bacterial species for anti-biofouling experiments. Results indicated that the composite PES membranes, which incorporated the OMWCNT and AG, possessed significantly stronger hydrophilicity and negative surface charge as evidenced by water contact angle and zeta potential data, respectively, when compared to plain PES membranes. Furthermore atomic force microscopy analysis showed that the PES/OMWCNT membranes exhibited significantly lower surface roughness values. Together, these membrane surface features were held responsible for the anti-adhesive nature of the hybrid membranes seen during biofouling tests. Importantly, the prepared membranes were able to inhibit bacterial colonization upon incubation with both Gram-positive and Gram-negative bacterial suspensions. The PES/OMWCNT membranes also presented more resilient normalized flux values when compared to neat PES and commercial membrane samples during filtration of both bacterial suspensions and real treated sewage effluents. Taken together, the results of this study allude to OMWCNT and AG as promising additives, for incorporation into polymeric membranes to enhance biofouling resistance.
Membrane Fouling Mechanisms in Combined Microfiltration-Coagulation of Algal Rich Water Applying Ceramic Membranes
Authors: Park Kitae , Kim Pooreum , Kim Hyoung Gun , Kim JiHoon
Published: February 2019

In this paper, we investigated the membrane fouling mechanism according to the coagulant dosage in algal rich water using a ceramic membrane. The algae that were used in this experiment were Microcystis sp. of cyanobacteria, and the fouling mechanism was analyzed through irrigation and filtration resistance through a constant flow operation. The experimental results showed that the filtration resistance decreased as the coagulant dosage increased, but the irreversibility at above optimum coagulant dosage increased. Additionally, as the coagulant dosage increased, the resistance value due to cake and adsorption contamination decreased, and membrane fouling by adsorption was dominant in comparison with cake fouling and adsorption fouling. The specific cake resistance was decreased as the coagulant dosage increased. The characteristics of the cake layer according to the coagulant dosage were found to loosely form the cake layer by increasing micro-size algae as the coagulant dosage increased. The results of this experiment confirmed the membrane fouling mechanism according to coagulant dosage when the ceramic membrane filtered algal rich water.
First-Principles Calculation for the Influence of C and O on the Mechanical Properties of γ-TiAl Alloy at High Temperature
Authors: Wang Jiahua , Lu Yong , Shao Xiaohong
Published: February 2019

The elastic constants of temperature dependence, thermal expansion coefficient and phonon dispersion relations of γ-TiAl doped with C/O have been investigated using first-principles calculations in order to gain insight into the mechanical performance of γ-TiAl in cases of high temperature. This study shows that γ-TiAl maintains stability at high temperatures introduced by C or O atoms. Importantly, the hardness increases and retains excellent resistance to external pressure. The results indicate that even if the TiAl alloy is doped with C or O atoms, it can also exhibit excellent mechanical properties at a high temperature.
The Impacts of Matrix Compositions on Nanopore Structure and Fractal Characteristics of Lacustrine Shales from the Changling Fault Depression, Songliao Basin, China
Authors: Li Zhuo , Liang Zhikai , Jiang Zhenxue , Gao Fenglin , Zhang Yinghan , Yu Hailong , Xiao Lei , Yang Youdong
Published: February 2019

The Lower Cretaceous Shahezi shales are the targets for lacustrine shale gas exploration in Changling Fault Depression (CFD), Southern Songliao Basin. In this study, the Shahezi shales were investigated to further understand the impacts of rock compositions, including organic matters and minerals on pore structure and fractal characteristics. An integrated experiment procedure, including total organic carbon (TOC) content, X-ray diffraction (XRD), field emission-scanning electron microscope (FE-SEM), low pressure nitrogen physisorption (LPNP), and mercury intrusion capillary pressure (MICP), was conducted. Seven lithofacies can be identified according to on a mineralogy-based classification scheme for shales. Inorganic mineral hosted pores are the most abundant pore type, while relatively few organic matter (OM) pores are observed in FE-SEM images of the Shahezi shales. Multimodal pore size distribution characteristics were shown in pore width ranges of 0.5–0.9 nm, 3–6 nm, and 10–40 nm. The primary controlling factors for pore structure in Shahezi shales are clay minerals rather than OM. Organic-medium mixed shale (OMMS) has the highest total pore volumes (0.0353 mL/g), followed by organic-rich mixed shale (ORMS) (0.02369 mL/g), while the organic-poor shale (OPS) has the lowest pore volumes of 0.0122 mL/g. Fractal dimensions D1 and D2 (at relative pressures of 0–0.5 and 0.5–1 of LPNP isotherms) were obtained using the Frenkel–Halsey–Hill (FHH) method, with D1 ranging from 2.0336 to 2.5957, and D2 between 2.5779 and 2.8821. Fractal dimensions are associated with specific lithofacies, because each lithofacies has a distinctive composition. Organic-medium argillaceous shale (OMAS), rich in clay, have comparatively high fractal dimension D1. In addition, organic-medium argillaceous shale (ORAS), rich in TOC, have comparatively high fractal dimension D2. OPS shale contains more siliceous and less TOC, with the lowest D1 and D2. Factor analysis indicates that clay contents is the most significant factor controlling the fractal dimensions of the lacustrine Shahezi shale.
Antiplasmodial and Cytotoxic Cytochalasins from an Endophytic Fungus, Nemania sp. UM10M, Isolated from a Diseased Torreya taxifolia Leaf
Authors: Kumarihamy Mallika , Ferreira Daneel , Croom Edward M. , Sahu Rajnish , Tekwani Babu L. , Duke Stephen O. , Khan Shabana , Techen Natascha , Nanayakkara N. P. Dhammika
Published: February 2019

Bioassay-guided fractionation of an EtOAc extract of the broth of the endophytic fungus Nemania sp. UM10M (Xylariaceae) isolated from a diseased Torreya taxifolia leaf afforded three known cytochalasins, 19,20-epoxycytochalasins C (1) and D (2), and 18-deoxy-19,20-epoxy-cytochalasin C (3). All three compounds showed potent in vitro antiplasmodial activity and phytotoxicity with no cytotoxicity to Vero cells. These compounds exhibited moderate to weak cytotoxicity to some of the cell lines of a panel of solid tumor (SK-MEL, KB, BT-549, and SK-OV-3) and kidney epithelial cells (LLC-PK11). Evaluation of in vivo antimalarial activity of 19,20-epoxycytochalasin C (1) in a mouse model at 100 mg/kg dose showed that this compound had weak suppressive antiplasmodial activity and was toxic to animals.
Pre-grafted Group on PE Surface by DBD Plasma and Its Influence on the Oxygen Permeation with Coated SiOx
Authors: Li Hua , Yang Lizhen , Wang Zhengduo , Liu Zhongwei , Chen Qiang
Published: February 2019

In this paper, we report on polyethylene (PE) film modified by atmospheric dielectric barrier discharge (DBD) plasma prior to the deposition of SiOx coating to improve its barrier properties. Three kinds of monomers: allylamine, acrylic acid, and ethanol, are used to modify the PE surface. For comparison, Ar and O2 plasma pre-treatments are also performed. It is found that with the addition of a monomer in the Ar DBD plasma, the grafted active groups on PE surfaces lead to dense, pinhole-free growth of the SiOx film. The oxygen transmission rate (OTR) decreases from 700 cc/m2·day·atm. for the pristine to ca. 70 cc/m2·day·atm. for the pretreatment-coated PE, which is more than a 10-fold reduction. The relationship between the grafted monomer and the great decrease of OTR is then explored via chemical composition by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and via morphology observation by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The results show that the grafted functional groups of -NH2, -COOH and -OH increase the surface energy and promote the nucleation of Si–O radicals on polymeric surfaces, and the formation of network and cage structures in SiOx film contributes to the significant improvement of OTR.
Organic Salts and Merrifield Resin Supported [PM12O40]3− (M = Mo or W) as Catalysts for Adipic Acid Synthesis
Authors: Pisk Jana , Agustin Dominique , Poli Rinaldo
Published: February 2019

Adipic acid (AA) was obtained by catalyzed oxidation of cyclohexene, epoxycyclohexane, or cyclohexanediol under organic solvent-free conditions using aqueous hydrogen peroxide (30%) as an oxidizing agent and molybdenum- or tungsten-based Keggin polyoxometalates (POMs) surrounded by organic cations or ionically supported on functionalized Merrifield resins. Operating under these environmentally friendly, greener conditions and with low catalyst loading (0.025% for the molecular salts and 0.001–0.007% for the supported POMs), AA could be produced in interesting yields.
Increased Selectivity of Novozym 435 in the Asymmetric Hydrolysis of a Substrate with High Hydrophobicity Through the Use of Deep Eutectic Solvents and High Substrate Concentrations
Authors: Fredes Yerko , Chamorro Lesly , Cabrera Zaida
Published: February 2019

The effects of the reaction medium and substrate concentration were studied on the selectivity of Novozym 435 using the asymmetric hydrolysis of dimethyl-3-phenylglutarate as a model reaction. Results show that the use of choline chloride ChCl:urea/phosphate buffer 50% (v/v) as a reaction medium increased the selectivity of Novozym 435 by 16% (e.e = 88%) with respect to the one in 100% phosphate buffer (e.e = 76%). Best results were obtained when high substrate concentrations (well above the solubility limit, 27-fold) and ChCl:urea/phosphate buffer 50% (v/v) as reaction medium at pH 7 and 30 °C were used. Under such conditions, the R-monoester was produced with an enantiomeric purity of 99%. Novozym 435 was more stable in ChCl:urea/phosphate buffer 50% (v/v) than in phosphate buffer, retaining a 50% of its initial activity after 27 h of incubation at pH 7 and 40 °C. Results suggest that the use of deep eutectic solvents (ChCl:urea/phosphate buffer) in an heterogeneous reaction system (high substrate concentration) is a viable and promising strategy for the synthesis of chiral drugs from highly hydrophobic substrates.
Methylmalonic Acid and Homocysteine as Indicators of Vitamin B12 Deficiency in Patients with Gastric Cancer after Gastrectomy
Authors: Lee Sae-Mi , Oh Jongwon , Chun Mi-Ryung , Lee Soo-Youn
Published: February 2019

Vitamin B12 deficiency is a common complication in patients after gastrectomy. Elevated methylmalonic acid (MMA) and homocysteine are better indications of vitamin B12 deficiency than vitamin B12 serum level. We compared MMA and homocysteine levels of patients with gastric cancer after gastrectomy (n = 151) with controls (n = 142) and evaluated the prevalence of vitamin B12 deficiency using MMA and homocysteine in patients. MMA and homocysteine levels were significantly higher (p < 0.05) in patients with gastric cancer after gastrectomy. Of the 151 patients assessed after gastrectomy, 32 patients (21.2%) were vitamin B12 deficient as defined by serum MMA levels > 350 nmol/L, and 8 patients (5.3%) were vitamin B12 deficient as defined by serum homocysteine levels > 15 μmol/L. Both MMA and homocysteine levels were elevated in 7 patients. Among 33 patients with elevated MMA or homocysteine levels, 8 patients (24.2%) were vitamin B12 deficient based on a serum vitamin B12 level < 200 pg/mL. Additionally, levels of MMA and homocysteine were compared pre- and post-gastrectomy in 27 patients. The median MMA level was higher in patients with post-gastrectomy compared to pre-gastrectomy, while the median serum homocysteine level was not significantly different. These results indicate that using serum vitamin B12 levels alone may fail to detect vitamin B12 deficiency. Additional assessments of MMA and homocysteine levels are useful to evaluate possible vitamin B12 deficiency in patients who underwent a gastrectomy, and MMA is a better indicator than homocysteine to detect early changes in vitamin B12 levels.
Chemical Characterization and Antiplatelet Potential of Bioactive Extract from Tomato Pomace (Byproduct of Tomato Paste)
Authors: Palomo Ivan , Concha-Meyer Anibal , Lutz Mariane , Said Macarena , Sáez Bárbara , Vásquez Adriana , Fuentes Eduardo
Published: February 2019

We examined the ability of tomato pomace extract (by-product) to affect platelet aggregation in healthy humans (clinical pilot study). In phase 1 the tolerance of participants (n = 15; 5 per dose level) ingesting tomato pomace extract across three dose levels (1, 2.5, and 10 g) was evaluated. Phase 2 was a single-blind, placebo-controlled, parallel design human (male, n = 99; 33 per group) pilot intervention trial investigating the acute and repeated dose effects (5 days) of different doses of tomato pomace extract (1 g, 2.5 g or placebo) on platelet aggregation ex vivo. Various flavonoids (coumaric acid, floridzin, floretin, procyanidin B2, luteolin-7-O-glucoside, kaempferol, and quercitin) and nucleosides (adenosine, inosine, and guanosine) were identified in the tomato pomace extract. The clinical study showed that the daily consumption of 1 g of aqueous extract of tomato pomace for 5 days exerted an inhibitory activity on platelet aggregation.
Topological Aspects of the Design of Nanocarriers for Therapeutic Peptides and Proteins
Authors: Knauer Nadezhda , Pashkina Ekaterina , Apartsin Evgeny
Published: February 2019

Supramolecular chemistry holds great potential for the design of versatile and safe carriers for therapeutic proteins and peptides. Nanocarriers can be designed to meet specific criteria for given application (exact drug, administration route, target tissue, etc.). However, alterations in the topology of formulation components can drastically change their activity. This is why the supramolecular topology of therapeutic nanoconstructions has to be considered. Herein, we discuss several topological groups used for the design of nanoformulations for peptide and protein delivery: modification of polypeptide chains by host-guest interactions; packaging of proteins and peptides into liposomes; complexation and conjugation with dendrimers. Each topological type has its own advantages and disadvantages, so careful design of nanoformulations is needed. Ideally, each case where nanomedicine is needed requires a therapeutic construction specially created for that taking into account features of the administration route, target tissue, or organ, properties of a drug, its bioavailability, etc. The wide number of studies in the field of protein delivery by supramolecular and nanocarriers for proteins and peptides evidence their increasing potential for different aspects of the innovative medicine. Although significant progress has been achieved in the field, there are several remaining challenges to be overcome in future.
Preparation and Characterization of Erythrocyte Membrane-Camouflaged Berberine Hydrochloride-Loaded Gelatin Nanoparticles
Authors: Su Jing , Zhang Ran , Lian Yumei , Kamal Zul , Cheng Zhongyao , Qiu Yujiao , Qiu Mingfeng
Published: February 2019

The discovery of a new pharmacological application of berberine hydrochloride (BH) made it more clinically valuable. However, the further development of BH was hampered by its short half-life and side effects after intravenous injection. To overcome these problems, a novel BH delivery system was developed using natural red blood cell membrane-camouflaged BH-loaded gelatin nanoparticles (RBGPs) to reduce the toxicity associated with injections and achieve sustained release. The size of the RBGPs was 260.3 ± 4.1 nm, with an obvious core–shell structure, and the membrane proteins of the RBGPs were mostly retained. The RBGP system showed significant immune-evading capabilities and little cytotoxicity to human embryonic kidney (HEK) 293T cells and LO2 cells. Finally, RBGPs improved the sustained releasing effect of BH significantly. When the cumulative release time reached 120 h, the cumulative release rate of RBGPs was 78.42%. In brief, RBGPs hold the potential to achieve long circulation and sustained-release of BH, avoid side effects caused by high plasma concentration in common injection formulations, and broaden the clinical applications of BH.
Modeling of High-Efficiency Multi-Junction Polymer and Hybrid Solar Cells to Absorb Infrared Light
Authors: Khanam Jobeda J. , Foo Simon Y.
Published: February 2019

In this paper, we present our work on high-efficiency multi-junction polymer and hybrid solar cells. The transfer matrix method is used for optical modeling of an organic solar cell, which was inspired by the McGehee Group in Stanford University. The software simulation calculates the optimal thicknesses of the active layers to provide the best short circuit current (JSC) value. First, we show three designs of multi-junction polymer solar cells, which can absorb sunlight beyond the 1000 nm wavelengths. Then we present a novel high-efficiency hybrid (organic and inorganic) solar cell, which can absorb the sunlight with a wavelength beyond 2500 nm. Approximately 12% efficiency was obtained for the multi-junction polymer solar cell and 20% efficiency was obtained from every two-, three- and four-junction hybrid solar cell under 1 sun AM1.5 illumination.
We Have Come Back Home: The Spanish-Moroccan Community, Collective Memory, and Sacred Spaces in Contemporary Spain
Authors: Ouassini Anwar
Published: February 2019

This paper examines the role of Islamic sacred spaces in Spanish-Moroccan identity negotiations in contemporary Madrid, Spain. In doing so, I explore how these sacred sites produce diverse meanings and practices that resist the Spanish states hegemonic narratives of place. I argue that the multilayered resistance via the “memory” and “place” of these sacred sites ostensibly reconciles and situates Spanish-Moroccans within the larger Spanish imagined community. The paper will first discuss the trans-local experiences of the Spanish-Moroccan community and how their liminal state of being neither “here or there” necessitates an anchor (Muslim sacred spaces) to the new home context. I will then outline a brief historical narrative of the Muslim presence in Spain and then analyze the meanings attached to the sacrality of Islamic monuments and mosques to the Spanish-Moroccan community. Finally, the paper will explore how the historical memories and their discursive meanings attached to these sacred sites allow Spanish-Moroccans to produce counterhegemonic frameworks that challenge and reshape nationalistic spaces.
A Novel Approach for the Detection of Developing Thunderstorm Cells
Authors: Müller Richard , Haussler Stéphane , Jerg Matthias , Heizenreder Dirk
Published: February 2019

This study presents a novel approach for the early detection of developing thunderstorms. To date, methods for the detection of developing thunderstorms have usually relied on accurate Atmospheric Motion Vectors (AMVs) for the estimation of the cooling rates of convective clouds, which correspond to the updraft strengths of the cloud objects. In this study, we present a method for the estimation of the updraft strength that does not rely on AMVs. The updraft strength is derived directly from the satellite observations in the SEVIRI water vapor channels. For this purpose, the absolute value of the vector product of spatio-temporal gradients of the SEVIRI water vapor channels is calculated for each satellite pixel, referred to as Normalized Updraft Strength (NUS). The main idea of the concept is that vertical updraft leads to NUS values significantly above zero, whereas horizontal cloud movement leads to NUS values close to zero. Thus, NUS is a measure of the strength of the vertical updraft and can be applied to distinguish between advection and convection. The performance of the method has been investigated for two summer periods in 2016 and 2017 by validation with lightning data. Values of the Critical Success Index (CSI) of about 66% for 2016 and 60% for 2017 demonstrate the good performance of the method. The Probability of Detection (POD) values for the base case are 81.8% for 2016 and 89.2% for 2017, respectively. The corresponding False Alarm Ratio (FAR) values are 22.6% (2016) and 36.4% (2017), respectively. In summary, the method has the potential to reduce forecast lead time significantly and can be quite useful in regions without a well-maintained radar network.
Landsat 4, 5 and 7 (1982 to 2017) Analysis Ready Data (ARD) Observation Coverage over the Conterminous United States and Implications for Terrestrial Monitoring
Authors: Egorov Alexey , Roy David , Zhang Hankui , Li Zhongbin , Yan Lin , Huang Haiyan
Published: February 2019

The Landsat Analysis Ready Data (ARD) are designed to make the U.S. Landsat archive straightforward to use. In this paper, the availability of the Landsat 4 and 5 Thematic Mapper (TM) and Landsat 7 Enhanced Thematic Mapper Plus (ETM+) ARD over the conterminous United States (CONUS) are quantified for a 36-year period (1 January 1982 to 31 December 2017). Complex patterns of ARD availability occur due to the satellite orbit and sensor geometry, cloud, sensor acquisition and health issues and because of changing relative orientation of the ARD tiles with respect to the Landsat orbit paths. Quantitative per-pixel and summary ARD tile results are reported. Within the CONUS, the average annual number of non-cloudy observations in each 150 × 150 km ARD tile varies from 0.53 to 16.80 (Landsat 4 TM), 11.08 to 22.83 (Landsat 5 TM), 9.73 to 21.72 (Landsat 7 ETM+) and 14.23 to 30.07 (all three sensors). The annual number was most frequently only 2 to 4 Landsat 4 TM observations (36% of the CONUS tiles), increasing to 14 to 16 Landsat 5 TM observations (26% of tiles), 12 to 14 Landsat 7 ETM+ observations (31% of tiles) and 18 to 20 observations (23% of tiles) when considering all three sensors. The most frequently observed ARD tiles were in the arid south-west and in certain mountain rain shadow regions and the least observed tiles were in the north-east, around the Great Lakes and along parts of the north-west coast. The quality of time series algorithm results is expected to be reduced at ARD tiles with low reported availability. The smallest annual number of cloud-free observations for the Landsat 5 TM are over ARD tile h28v04 (northern New York state), for Landsat 7 ETM+ are over tile h25v07 (Ohio and Pennsylvania) and for Landsat 4 TM are over tile h22v08 (northern Indiana). The greatest annual number of cloud-free observations for the Landsat 5 TM and 7 ETM+ ARD are over southern California ARD tile h04v11 and for the Landsat 4 TM are over southern Arizona tile h06v13. The reported results likely overestimate the number of good surface observations because shadows and cirrus clouds were not considered. Implications of the findings for terrestrial monitoring and future ARD research are discussed.
Glacier Facies Mapping Using a Machine-Learning Algorithm: The Parlung Zangbo Basin Case Study
Authors: Zhang Jingxiao , Jia Li , Menenti Massimo , Hu Guangcheng
Published: February 2019

Glaciers in the Tibetan Plateau are an important indicator of climate change. Automatic glacier facies mapping utilizing remote sensing data is challenging due to the spectral similarity of supraglacial debris and the adjacent bedrock. Most of the available glacier datasets do not provide the boundary of clean ice and debris-covered glacier facies, while debris-covered glacier facies play a key role in mass balance research. The aim of this study was to develop an automatic algorithm to distinguish ice cover types based on multi-temporal satellite data, and the algorithm was implemented in a subregion of the Parlung Zangbo basin in the southeastern Tibetan Plateau. The classification method was built upon an automated machine learning approach: Random Forest in combination with the analysis of topographic and textural features based on Landsat-8 imagery and multiple digital elevation model (DEM) data. Very high spatial resolution Gao Fen-1 (GF-1) Panchromatic and Multi-Spectral (PMS) imagery was used to select training samples and validate the classification results. In this study, all of the land cover types were classified with overall good performance using the proposed method. The results indicated that fully debris-covered glaciers accounted for approximately 20.7% of the total glacier area in this region and were mainly distributed at elevations between 4600 m and 4800 m above sea level (a.s.l.). Additionally, an analysis of the results clearly revealed that the proportion of small size glaciers (<1 km2) were 88.3% distributed at lower elevations compared to larger size glaciers (≥1 km2). In addition, the majority of glaciers (both in terms of glacier number and area) were characterized by a mean slope ranging between 20° and 30°, and 42.1% of glaciers had a northeast and north orientation in the Parlung Zangbo basin.
Measurement of Human Gait Symmetry using Body Surface Normals Extracted from Depth Maps
Authors: Nguyen Trong-Nguyen , Huynh Huu-Hung , Meunier Jean
Published: February 2019

In this paper, we introduce an approach for measuring human gait symmetry where the input is a sequence of depth maps of subject walking on a treadmill. Body surface normals are used to describe 3D information of the walking subject in each frame. Two different schemes for embedding the temporal factor into a symmetry index are proposed. Experiments on the whole body, as well as the lower limbs, were also considered to assess the usefulness of upper body information in this task. The potential of our method was demonstrated with a dataset of 97,200 depth maps of nine different walking gaits. An ROC analysis for abnormal gait detection gave the best result ( AUC = 0.958 ) compared with other related studies. The experimental results provided by our method confirm the contribution of upper body in gait analysis as well as the reliability of approximating average gait symmetry index without explicitly considering individual gait cycles for asymmetry detection.