bluuebunny/crossref_metadata_2025 huggingface dataset:
Italian Offshore Platform and Depleted Reservoir Conversion in the Energy Transition Perspective
Authors: Carpignano Andrea , Gerboni Raffaella , Mezza Alessio , Pirri Candido Fabrizio , Sacco Adriano , Sassone Daniele , Suriano Alessandro , Uggenti Anna Chiara , Verga Francesca , Viberti Dario
Published: August 2023

New hypotheses for reusing platforms reaching their end-of-life have been investigated in several works, discussing the potential conversions of these infrastructures from recreational tourism to fish farming. In this perspective paper, we discuss the conversion options that could be of interest in the context of the current energy transition, with reference to the off-shore Italian scenario. The study was developed in support of the development of a national strategy aimed at favoring a circular economy and the reuse of existing infrastructure for the implementation of the energy transition. Thus, the investigated options include the onboard production of renewable energy, hydrogen production from seawater through electrolyzers, CO2 capture and valorization, and platform reuse for underground fluid storage in depleted reservoirs once produced through platforms. Case histories are developed with reference to a typical, fictitious platform in the Adriatic Sea, Italy, to provide an engineering-based approach to these different conversion options. The coupling of the platform with the underground storage to set the optimal operational conditions is managed through the forecast of the reservoir performance, with advanced numerical models able to simulate the complexity of the phenomena occurring in the presence of coupled hydrodynamic, geomechanical, geochemical, thermal, and biological processes. The results of our study are very encouraging, because they reveal that no technical, environmental, or safety issues prevent the conversion of offshore platforms into valuable infrastructure, contributing to achieving the energy transition targets, as long as the selection of the conversion option to deploy is designed taking into account the system specificity and including the depleted reservoir to which it is connected when relevant. Socio-economic issues were not investigated, as they were out of the scope of the project.
Public Health Research Priorities for Fungal Diseases: A Multidisciplinary Approach to Save Lives
Authors: Smith Dallas J. , Gold Jeremy A. W. , Benedict Kaitlin , Wu Karen , Lyman Meghan , Jordan Alexander , Medina Narda , Lockhart Shawn R. , Sexton D. Joseph , Chow Nancy A. , Jackson Brendan R. , Litvintseva Anastasia P. , Toda Mitsuru , Chiller Tom
Published: August 2023

Fungal infections can cause severe disease and death and impose a substantial economic burden on healthcare systems. Public health research requires a multidisciplinary approach and is essential to help save lives and prevent disability from fungal diseases. In this manuscript, we outline the main public health research priorities for fungal diseases, including the measurement of the fungal disease burden and distribution and the need for improved diagnostics, therapeutics, and vaccines. Characterizing the public health, economic, health system, and individual burden caused by fungal diseases can provide critical insights to promote better prevention and treatment. The development and validation of fungal diagnostic tests that are rapid, accurate, and cost-effective can improve testing practices. Understanding best practices for antifungal prophylaxis can optimize prevention in at-risk populations, while research on antifungal resistance can improve patient outcomes. Investment in vaccines may eliminate certain fungal diseases or lower incidence and mortality. Public health research priorities and approaches may vary by fungal pathogen.
Antifungal Susceptibility Pattern of Candida glabrata from a Referral Center and Reference Laboratory: 2012–2022
Authors: Chesdachai Supavit , Yetmar Zachary A. , Ranganath Nischal , Everson Jenna J. , Wengenack Nancy L. , Abu Saleh Omar M.
Published: August 2023

The prevalence of invasive candidiasis caused by non-Candida albicans has rapidly increased. Candida glabrata (Nakaseomyces glabrata) is an important pathogen associated with substantial mortality. Our study examined the antifungal temporal susceptibility of C. glabrata and cross-resistance/non-wild-type patterns with other azoles and echinocandins. Laboratory data of all adult patients with C. glabrata isolated from clinical specimens at the Mayo Clinic, Rochester, from 2012 to 2022 were collected. Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints were used. We obtained 1046 C. glabrata isolates from 877 patients. Using CLSI and EUCAST breakpoints, 187 (17.9%) isolates and 256 (24.5%) isolates were fluconazole-resistant, respectively. Focusing on C. glabrata bloodstream infections, fluconazole-resistance ranged from 16 to 22%. Among those 187 fluconazole-resistant isolates, 187 (100%) and 184 (98.4%) isolates were also voriconazole and posaconazole non-wild-type, respectively, with 97 (51.9%) isolates deemed non-wild type for itraconazole. The fluconazole susceptibility pattern has not changed over the past decade. The proportion of fluconazole-resistant C. glabrata is relatively high, which could be due to the complexity of patients and fluconazole exposure. Itraconazole appears to be a compelling step-down therapy for fluconazole-resistant C. glabrata, given the high proportion of wild-type isolates. Further research to examine clinical outcomes is warranted.
Long-Term Impact of Soil and Water Conservation Measures on Soil Erosion in a Tunisian Semi-Arid Watershed
Authors: Jarray Fathia , Hermassi Taoufik , Mechergui Mohamed , Zucca Claudio , Le Quang Bao
Published: August 2023

Understanding and long-term assessment of the efficacy of soil and water conservation (SWC) techniques is essential for sustainable watershed management. There have been few studies measuring the long-term impact of SWC on soil erosion at the catchment scale due to a lack of historical data. This research aims to measure and analyze the impact of SWC interventions on sediment yield using the Soil and Water Assessment Tool (SWAT) in the Wadi Rmel watershed between 2000 and 2020. The study approach has simultaneously three main aspects: (i) long-term and temporally dynamic, (ii) large-scale landscape distribution, and (iii) empirical evidence of impacts induced by terracing measures. Historical data on SWC in 2000, 2005, 2010, 2015, and 2020 were used for running the model. The monthly calibration (2001–2014) using the SUFI2 algorithm was implemented with 22 input parameters and displayed a good model performance, with a Nash–Sutcliffe efficiency (NSE) of 0.61 for daily runoff and a coefficient of determination (R2) of 0.66. A satisfactory result was obtained for the monthly validation process (2015–2020) with NSE value equal to 0.83 and R2 value of 0.85. The result showed the increase in terracing areas led to a cumulative decrease in watershed sediment yield in long-term, i.e., the reduced erosion per one hectare of the expanded terracing area after 10–20 years were about 9–33 times higher than those in the first 5 years. This finding suggests that maximal benefits of SWC should be expected in the long-term, i.e., beyond a decade. Additionally, the study revealed variations in sediment yield contribution among sub-basins, with the southwestern region being the degradation hotspot areas (having erosion exceeding tolerable thresholds) needing prioritization for erosion controls. These findings enable stakeholders to plan effective management in semi-arid wheat-based agricultural areas with scarce data.
Effects of Artificial Green Land on Land–Atmosphere Interactions in the Taklamakan Desert
Authors: Abudukade Silalan , Yang Fan , Liu Yongqiang , Mamtimin Ali , Gao Jiacheng , Ma Mingjie , Wang Wenbiao , Cui Zhengnan , Wang Yu , Zhang Kun , Song Meiqi , Zhang Jiantao
Published: August 2023

Land–atmosphere interactions are influenced by the earth’s complex underlying subsurface, which in turn indirectly affects atmospheric motion and climate change. Human activities are increasingly exerting an influence on desert ecosystems, and artificial green land with clear functional orientation has been established in many desert areas. Consequently, the previously dominant, shifting, sand-covered, underlying surface in these desert regions is gradually transforming. This transformation has significant implications for the characteristics of land–atmosphere interactions, causing them to deviate from their original state. At present, existing studies still have not presented a systematic understanding of this change and have ignored the impact of human activities on land–atmosphere interactions in artificial green land. To address these research gaps, this study specifically targets artificial green land in the Tazhong region of Taklamakan Desert. We carried out observation experiments on land–atmosphere interactions in three different functional units from outside to inside: natural shifting sands, the shelter forest, and the living area. We also analyzed the differences and attribution of land–atmosphere interactions characteristics of different functional units. Compared with the natural shifting sands, the daily average maximum values of wind speed in the shelter forest decreased by 78%, and the daily average maximum air temperature and soil (0 cm) temperature decreased by 2.6 °C and 7 °C, respectively. Additionally, the soil moisture level was significantly increased throughout the green land due to the shelter forest. The surface albedo experienced a decrease, with an annual average of 0.21. Furthermore, the aerodynamic roughness and bulk transport coefficient increased by two orders of magnitude. The daily average maximum values of sensible heat flux and soil heat flux (G05) decreased by 18.7% and 75%, respectively, and the daily average maximum value of latent heat flux increased by 70.3%. This effectively improved the microclimate environment of the green land. The living area was greatly reduced by the shelter forest coverage and influenced by the buildings. Consequently, the environmental improvement was not as large as it was inside the shelter forest. However, it still provided a good shelter for production and living in the desert area. Throughout the year, a total of 4.60 × 105 t water was consumed through evapotranspiration in the artificial green land. The findings of this study have the potential to enhance our comprehension of land–atmosphere interactions in desert regions, thereby offering valuable insights for the establishment and effective management of artificial desert green lands.
The Influence of Perceived Physical and Aesthetic Quality of Rural Settlements on Tourists’ Preferences—A Case Study of Zhaoxing Dong Village
Authors: Jiang Shuai , Ma Haoran , Yang Ling , Luo Shixian
Published: August 2023

Rural settlements, as crucial human habitats, encompass various values such as residential living, cultural tourism, and industrial development. This paper investigates the environmental physical and aesthetic factors perceived by tourists, which influence their preferences for rural settlement environments. Previous studies have predominantly focused on evaluating the impacts of physical or aesthetic factors on tourists’ environmental preferences, with limited research simultaneously examining their combined effects. To reduce this research gap, we selected Zhaoxing Dong Village in China, characterized by typical rural environmental traits, and collected 450 valid questionnaires. The questionnaire data underwent correlation analysis and multiple linear regression analysis. The results indicate that when considering only environmental physical quality factors, most of the physical quality factors are significantly correlated with tourists’ preferences. Among them, “visual quality” shows the highest correlation, followed by “facility” and “maintenance”, while “security” shows the lowest correlation. When aesthetic quality factors are added to the model as independent variables, they enhance the explanatory power of the model and exhibit more significant associations compared to the relationship between physical quality factors and preferences. Among the aesthetic quality factors, “multisensory” and “sublime” demonstrate the highest correlation, whereas “diversity” shows the lowest correlation. The current study demonstrates the validity of the two scales for measuring tourists’ perceived levels of physical and aesthetic quality in rural settlement environments. These findings contribute to the effective utilization of environmental capital within rural settlements and provide guidance for rural settlement planning and design.
Green Supply Chain Management: The Effect of Procurement Sustainability on Reverse Logistics
Authors: Letunovska Nataliia , Offei Felix Amoako , Junior Prince Amoh Junior , Lyulyov Oleksii , Pimonenko Tetyana , Kwilinski Aleksy
Published: August 2023

Background: Sustainable procurement is about considering social and environmental factors alongside financial factors in making procurement decisions and purchasing goods and services that take into account the social, economic, and environmental impact that such purchasing has on people and communities. This study was carried out to address the effects of procurement sustainability on reverse logistics. It considered four objectives: to establish the relationship between environmental procurement sustainability and reverse logistics, to examine the relationship between economic procurement sustainability and reverse logistics, to examine the relationship between social sustainability procurement and reverse logistics, and to examine the moderating role of government policy. Methods: This study applied structural equation modeling to check the research hypotheses. A purposive sampling technique was used. A structured questionnaire was used to gather primary data. The data gathered were analyzed using descriptive and inferential analysis. The data were analyzed in SPSS version 21. The study found that environmental, economic, and social procurement sustainability has a significant and positive effect on reverse logistics. Government policy moderates the relationships between environmental, economic, and social procurement sustainability and reverse logistics. Conclusions: This study recommends that organizations team up with environmental protection agencies to produce environmentally friendly products. Social interventions that can help protect society from damage should be acknowledged. Good economic policies that ensure that organizations increase their wealth should also be acknowledged.
Experimental Analysis of Rolling Torque and Thermal Inlet Shear Heating in Tapered Roller Bearings
Authors: Manjunath Manjunath , Fauconnier Dieter , Ost Wouter , De Baets Patrick
Published: August 2023

The investigation in this article focuses on the rolling resistance torque and thermal inlet shear factor in tapered roller bearings (TRBs) through systematic experiments using a modular test setup. TRBs typically operate under Elastohydrodynamic Lubrication (EHL) conditions. At sufficiently high speeds, the majority of rolling friction is due to a significant shift of the pressure centre in the EHL contact. While at lower speeds, sliding friction in the roller-rib contact becomes dominant, which operates under mixed lubrication conditions. Limited literature exists on the impact of inlet shear heating on effective lubricant temperature (Tin_c) and rolling friction in TRBs. To fill this gap, experimental measurements of the total frictional torque under axial loading at different speeds and oil temperatures are performed. With existing models for different friction contributions described in the literature, the rolling resistance due to EHL has been determined for various operating conditions. The effects of dimension-less speed (U), material (G), and load (W) parameters have also been investigated. Under fully flooded conditions, it was observed that the influence of material (G) and load (W) parameters on rolling friction is minor, while the impact of velocity (U) is significant. In the context of rolling resistance, the heating due to shear of the lubricant in the inlet zone plays a significant role. For higher rotational velocities, the estimated rotational torque reduction resulting from inlet shear heating was found to be approximately 6–8%.
Electrospun Scaffolds for Tissue Engineering: A Review
Authors: Flores-Rojas Guadalupe Gabriel , Gómez-Lazaro Bélen , López-Saucedo Felipe , Vera-Graziano Ricardo , Bucio Emilio , Mendizábal Eduardo
Published: August 2023

Tissue engineering and regenerative medicine have emerged as innovative approaches to enhance clinical outcomes by addressing tissue lesions and degenerations that can significantly impair organ function. Since human tissues have limited regenerative capacity, the field of regenerative medicine aims to restore damaged tissues and their functionalities. Recent decades have witnessed remarkable progress in materials science, tissue engineering, and medicine, leading to the development of regenerative engineering. This interdisciplinary field has revolutionized the production of artificial matrices, enabling the design of anatomically accurate structures with enhanced biocompatibility, bioabsorption, and cell adhesion. Among the techniques utilized for fabricating cellular scaffolds, the electrospinning of fibers stands out as an ideal approach due to its ability to mimic the characteristics of the extracellular matrix (ECM). Electrospun scaffolds exhibit distinct advantages, including a high surface area-to-volume ratio, exceptional porosity, uniformity, compositional diversity, structural flexibility, and the ease of functionalization with bioactive molecules for controlled release. These versatile properties allow for the creation of nanofiber scaffolds that closely resemble the architecture of the ECM. Consequently, they facilitate the transport of nutrients and oxygen to cells as well as the incorporation of growth factors to stimulate cell growth. These advancements open up a wide range of applications in the field of regenerative medicine.
Differentiating Chat Generative Pretrained Transformer from Humans: Detecting ChatGPT-Generated Text and Human Text Using Machine Learning
Authors: Katib Iyad , Assiri Fatmah Y. , Abdushkour Hesham A. , Hamed Diaa , Ragab Mahmoud
Published: August 2023

Recently, the identification of human text and ChatGPT-generated text has become a hot research topic. The current study presents a Tunicate Swarm Algorithm with Long Short-Term Memory Recurrent Neural Network (TSA-LSTMRNN) model to detect both human as well as ChatGPT-generated text. The purpose of the proposed TSA-LSTMRNN method is to investigate the model’s decision and detect the presence of any particular pattern. In addition to this, the TSA-LSTMRNN technique focuses on designing Term Frequency–Inverse Document Frequency (TF-IDF), word embedding, and count vectorizers for the feature extraction process. For the detection and classification processes, the LSTMRNN model is used. Finally, the TSA is employed for selecting the parameters for the LSTMRNN approach, which enables improved detection performance. The simulation performance of the proposed TSA-LSTMRNN technique was investigated on benchmark databases, and the outcome demonstrated the advantage of the TSA-LSTMRNN system over other recent methods with a maximum accuracy of 93.17% and 93.83% on human- and ChatGPT-generated datasets, respectively.
The Utility of 4D-CT Imaging in Primary Hyperparathyroidism Management in a Low-Volume Center
Authors: Murruste Marko , Kivilo Martin , Kase Karri , Kirsimägi Ülle , Tähepõld Annika , Tammiksaar Kaia
Published: August 2023

Background: Ultrasonography (US) and the 99mTc-sestamibi parathyroid scan (SPS) may have suboptimal accuracy when detecting the localization of enlarged parathyroid gland(s) (PTG). Therefore, the more accurate four-dimensional computed tomography scan (4D-CT) has been employed for PTG imaging. Currently, there is a paucity of data evaluating the utility of 4D-CT in low caseload settings. Aim and Objectives: To evaluate the impact of PTG imaging, using 4D-CT in conjunction with its intraoperatively displayed results, on the outcomes of surgical PTX. Materials and Methods: A single-center retrospective analysis of surgically treated patients with pHPT from 01/2010 to 01/2021 was conducted. An evaluation of the impact of the preoperative imaging modalities on the results of surgical treatment was carried out. Results: During the study period, 290 PTX were performed; 45 cases were excluded due to surgery for secondary, tertiary or recurrent HPT, or due to the use of alternative imaging techniques. The remaining 245 patients were included in the study. US was carried out for PTG imaging in 236 (96.3%), SPS in 93 (38.0%), and 4D-CT in 52 patients (21.2%). The use of 4D-CT was associated with a significantly higher rate of successful localization of enlarged PTG (49 cases, 94.2%) compared to US and SPS (74 cases, 31.4%, and 54 cases, 58.1%, respectively). We distinguished between three groups of patients based on preoperative imaging: (1) PTG lateralization via US or SPS in 106 (43.3%) cases; (2) precise localization of PTG via 4D-CT in 49 (20.0%) patients; and (3) in 90 cases (36.7%), PTG imaging failed to localize an enlarged gland. The group of 4D-CT localization had significantly shorter operative time, lower rate of simultaneous thyroid resections, as well as lower rate of removal of ≥2 PTG, compared to the other groups. The 4D-CT imaging was also associated with the lowest perioperative morbidity and with the lowest median PTH in the one month follow-up; however, compared to the other groups, these differences were statistically not significant. The implementation of 4D-CT (since 01/2018) was associated with a decrease in the need for redo surgery (from 11.5% to 7.3%) and significantly increased the annual case load of PTX at our institution (from 15.3 to 41.0) compared to the period before 4D-CT diagnostics. Conclusions: 4D-CT imaging enabled to precisely locate almost 95% of enlarged PTG in patients with pHPT. Accurate localization and intraoperatively displayed imaging results are useful guides for surgeons to make PTX a faster and safer procedure in a low-volume center.
Relationship between Behavior and Periodontal Health Self-Perception in Diabetic and Non-Diabetic Patients from Transylvania, Romania—A Self-Report Study, including The Desire to Use a Mobile App for Oral Care Improvements
Authors: Badea (Paun) Ariadna Georgiana , Bocanet Vlad Ioan , Badea Iulia Clara , Chifor Radu , Duma Livia Terezia , Borzan Cristina Maria
Published: August 2023

The study aimed to assess self-reported symptoms of periodontal disease (gingival bleeding, tooth mobility and halitosis) among diabetic and non-diabetic patients from Transylvania, Romania. Using statistical analysis methods, correlation between the aforementioned symptoms and diet, oral hygiene habits, stress, physical activity, and BMI (body mass index) were researched. Another secondary objective was to assess the impact of self-reported oral health, aesthetics, and halitosis on their life. Patients’ willingness to use a mobile app for generating awareness of oro-dental complications, improving their knowledge of oro-dental health, and reminding them to visit the dentist based on an awareness-raising function, was also assessed. Methods: The study was based on an original self-administered questionnaire, applied to 182 subjects, in an unselected, randomized manner. A total of 110 questionnaires were applied in two dental offices and 72 questionnaires were applied in a public clinic for diabetic patients from Cluj-Napoca, Romania. Results: Warning signs and symptoms relevant to periodontal disease were identified by respondents, having statistically significant associations with the declared oral health-care habits, including interdental aids, vicious habits (nail biting, bruxism), etc. Some of these periodontal signs could be correlated with a certain lifestyle, such as the perceived stress, smoking status, practicing maintenance sports, and alcohol consumption. A total of 66% of the non-diabetics and 68% of the diabetics of the surveyed subjects consider a software application for generating awareness of oro-dental complications useful, and are willing to pay around EUR 6 for it. Conclusions: Having a medical condition such as diabetes makes patients pay more attention to dental health compared with non-diabetic patients. Patients expressed their desire to use a software application to help them to be aware of their condition and for improving their self-report capabilities, including their oral-health-related status.
Hierarchical Culture as a Cross-Level Moderator of the Relationship between Transformational Leadership, Performance Feedback, and Employee Job Performance
Authors: Lee Michelle Chin Chin , Ding Alyssa Yen Lyn
Published: August 2023

Positive leadership such as transformational leadership is pertinent to employee positive work outcomes, yet not many studies have looked at how it functions under specific work environment (i.e., organizational culture). The present study investigates the process and extent to which transformational leadership relates to employee job performance through performance feedback under hierarchical culture within the Asian setting. Using a cross-sectional design with multilevel modelling, 60 private organizations were approached where 44 teams totaling to 256 participants were included in this study. Results reveal that performance feedback mediated transformational leadership and job performance. In the context of transformational leadership under hierarchical culture, a low hierarchical culture and a high transformational leadership showed the highest level of performance feedback while a low level hierarchical culture and a low transformational leadership showed the lowest level of performance feedback. A high hierarchical culture and a low transformational leadership showed the highest job performance while a low hierarchical culture and a low transformational leadership showed the lowest job performance. Organizations in Eastern countries may depend on the positive effects of transformational leadership for higher performance feedback among employees which positively relate to employee job performance.
Current Understanding of Potential Linkages between Biocide Tolerance and Antibiotic Cross-Resistance
Authors: Coombs Kent , Rodriguez-Quijada Cristina , Clevenger Jason O. , Sauer-Budge Alexis F.
Published: August 2023

Antimicrobials (e.g., antibiotics and biocides) are invaluable chemicals used to control microbes in numerous contexts. Because of the simultaneous use of antibiotics and biocides, questions have arisen as to whether environments commonly treated with biocides (e.g., hospitals, food processing, wastewater, agriculture, etc.) could act as a reservoir for the development of antibiotic cross-resistance. Theoretically, cross-resistance could occur if the mechanism of bacterial tolerance to biocides also resulted in antibiotic resistance. On the other hand, biocides would likely present a higher evolutionary barrier to the development of resistance given the different modes of action between biocides and antibiotics and the broad-based physicochemical effects associated with most biocides. Published studies have shown that the induction of biocide tolerance in a laboratory can result in cross-resistance to some antibiotics, most commonly hypothesized to be due to efflux pump upregulation. However, testing of environmental isolates for biocide tolerance and antibiotic cross-resistance has yielded conflicting results, potentially due to the lack of standardized testing. In this review, we aim to describe the state of the science on the potential linkage between biocide tolerance and antibiotic cross-resistance. Questions still remain about whether the directed evolution of biocide tolerance and the associated antibiotic cross-resistance in a laboratory are or are not representative of real-world settings. Thus, research should continue to generate informative data to guide policies and preserve these tools’ utility and availability.
Individual and Group-Based Effects of In Vitro Fiber Interventions on the Fecal Microbiota
Authors: Agamennone Valeria , van den Broek Tim J. , de Kat Angelino-Bart Alie , Hoevenaars Femke P. M. , van der Kamp Jan Willem , Schuren Frank H. J.
Published: August 2023

The development of microbiome-targeted strategies is limited by individual differences in gut microbiome composition and metabolic responses to interventions. In vitro models that can replicate this variation allow us to conduct pre-clinical studies and assess efficacy. This study describes the exposure of 16 individual fecal microbiota samples to 5 different fibers using an in vitro system for the anaerobic cultivation of bacteria. The individual microbiota differed in composition and metabolite profiles (short-chain fatty acids and branched-chain fatty acids) after incubation with the fibers. Furthermore, microbiota composition after fiber incubation was significantly different between subjects with good intestinal health and subjects with Inflammatory Bowel Disease (IBD). α-diversity was differently affected by dietary fibers; for example, exposure to psyllium resulted in increased diversity in the healthy group and in decreased diversity in the IBD group. Instead, the functional metabolic profile did not differ between the two groups. Finally, the combination of all fibers, tested on the microbiota from IBD subjects, resulted in stronger overall effects on both microbiota composition and metabolite production compared to the single fibers. These results confirm that incubation with dietary fiber results in different compositional and functional effects on individual microbiota and that in vitro models represent successful tools for studying individual fiber effects.
The Pathogenicity of Fusobacterium nucleatum Modulated by Dietary Fibers—A Possible Missing Link between the Dietary Composition and the Risk of Colorectal Cancer
Authors: Nawab Sadia , Bao Qelger , Ji Lin-Hua , Luo Qian , Fu Xiang , Fan Shuxuan , Deng Zixin , Ma Wei
Published: August 2023

The dietary composition has been approved to be strongly associated with the risk of colorectal cancer (CRC), one of the most serious malignancies worldwide, through regulating the gut microbiota structure, thereby influencing the homeostasis of colonic epithelial cells by producing carcinogens, i.e., ammonia or antitumor metabolites, like butyrate. Though butyrate-producing Fusobacterium nucleatum has been considered a potential tumor driver associated with chemotherapy resistance and poor prognosis in CRC, it was more frequently identified in the gut microbiota of healthy individuals rather than CRC tumor tissues. First, within the concentration range tested, the fermentation broth of F. nucleatum exhibited no significant effects on Caco-2 and NCM460 cells viability except for a notable up-regulation of the expression of TLR4 (30.70%, p < 0.0001) and Myc (47.67%, p = 0.021) and genes encoding proinflammatory cytokines including IL1B (197.57%, p < 0.0001), IL6 (1704.51%, p < 0.0001), and IL8 (897.05%, p < 0.0001) in Caco-2 cells exclusively. Although no marked effects of polydextrose or fibersol-2 on the growth of F. nucleatum, Caco-2 and NCM460 cells were observed, once culture media supplemented with polydextrose or fibersol-2, the corresponding fermentation broths of F. nucleatum significantly inhibited the growth of Caco-2 cells up to 48.90% (p = 0.0003, 72 h, 10%) and 52.96% (p = 0.0002, 72 h, 10%), respectively in a dose-dependent manner. These two kinds of fibers considerably promoted butyrate production of F. nucleatum up to 205.67% (p < 0.0001, 6% polydextrose at 24 h) and 153.46% (p = 0.0002, 6% fibersol-2 at 12 h), which explained why and how the fermentation broths of F. nucleatum cultured with fibers suppressing the growth of Caco-2 cells. Above findings indicated that dietary fiber determined F. nucleatum to be a carcinogenic or antitumor bacterium, and F. nucleatum played an important role in the association between the dietary composition, primarily the content of dietary fibers, and the risk of CRC.
Preliminary Study on Rapid and Simultaneous Detection of Viable Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella by PMA-mPCR in Food
Authors: Liu Yao , Wei Caijiao , Wan Hui , Sarengaowa  , Liang Xiaoping , Jiang Tao , Dong Yuhe , Zhao Xihong , Zhong Tian
Published: August 2023

Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella are major foodborne pathogens that are widespread in nature and responsible for several outbreaks of food safety accidents. Thus, a rapid and practical technique (PMA-mPCR) was developed for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella in pure culture and in a food matrix. To eliminate false positive results, propidium monoazide (PMA) was applied to selectively suppress the DNA amplification of dead cells. The results showed the optimum concentration of PMA is 5.0 µg/mL. The detection limit of this assay by mPCR was 103 CFU/mL in the culture broth, and by PMA-mPCR was 104 CFU/mL both in pure culture and a food matrix (milk and ground beef). In addition, the detection of mixed viable and dead cells was also explored in this study. The detection sensitivity ratio of viable and dead counts was less than 1:10. Therefore, the PMA-mPCR assay proposed here might provide an efficient detection tool for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella and also have great potential for the detection and concentration assessment of VBNC cells.
The Role of Cerium Valence in the Conversion Temperature of H2Ti3O7 Nanoribbons to TiO2-B and Anatase Nanoribbons, and Further to Rutile
Authors: Umek Polona , Dürrschnabel Michael , Molina-Luna Leopoldo , Škapin Srečo , Korošec Romana Cerc , Bittencourt Carla
Published: August 2023

CeO2-TiO2 is an important mixed oxide due to its catalytic properties, particularly in heterogeneous photocatalysis. This study presents a straightforward method to obtain 1D TiO2 nanostructures decorated with CeO2 nanoparticles at the surface. As the precursor, we used H2Ti3O7 nanoribbons prepared from sodium titanate nanoribbons by ion exchange. Two cerium sources with an oxidation state of +3 and +4 were used to obtain mixed oxides. HAADF–STEM mapping of the Ce4+-modified nanoribbons revealed a thin continuous layer at the surface of the H2Ti3O7 nanoribbons, while Ce3+ cerium ions intercalated partially between the titanate layers. The phase composition and morphology changes were monitored during calcination between 620 °C and 960 °C. Thermal treatment led to the formation of CeO2 nanoparticles on the surface of the TiO2 nanoribbons, whose size increased with the calcination temperature. The use of Ce4+ raised the temperature required for converting H2Ti3O7 to TiO2-B by approximately 200 °C, and the temperature for the formation of anatase. For the Ce3+ batch, the presence of cerium inhibited the conversion to rutile. Analysis of cerium oxidation states revealed the existence of both +4 and +3 in all calcined samples, regardless of the initial cerium oxidation state.
The Removal of Pollutants from Wastewater Using Magnetic Biochar: A Scientometric and Visualization Analysis
Authors: Li Chenyang , Zhang Chongbin , Zhong Shuang , Duan Jing , Li Ming , Shi Yan
Published: August 2023

In recent years, the use of magnetic biochar in wastewater treatment has shown significant effects and attracted scholars’ attention. However, due to the relatively short research time and the lack of systematic summaries, it is difficult to provide a more in-depth analysis. This study utilizes RStudio and CiteSpace software to comprehensively analyze the research trends and progress of magnetic biochar in wastewater treatment. The analysis of bibliometrics is performed on 551 relevant papers retrieved from the Web of Science, spanning the period between 2011 and 2022. The most influential countries, institutions, journals, disciplinary distribution, and top 10 authors and papers in this field have been identified. The latest dataset has been used for keyword clustering and burst analysis. The results indicated that: (1) Bin Gao is the most influential author in this field, and high-level journals such as Bioresource Technology are more inclined to publish articles in the field of magnetic biochar. (2) Research in this field has predominantly focused on the removal of heavy metals and organic compounds. Keyword burst analysis shows a shift in research direction towards the removal of complex organic pollutants recently. (3) For the future development of magnetic biochar, an environment-friendly approach, economic viability, and joint technology are the directions that need more exploration. Finally, this paper provides a summary of the various adsorption mechanisms of magnetic biochar and several common modification methods, aiming to assist scholars in their research endeavors.
Organotin (IV) Dithiocarbamate Compounds as Anticancer Agents: A Review of Syntheses and Cytotoxicity Studies
Authors: Abd Aziz Nurul Amalina , Awang Normah , Chan Kok Meng , Kamaludin Nurul Farahana , Mohamad Anuar Nur Najmi
Published: August 2023

Organotin (IV) dithiocarbamate has recently received attention as a therapeutic agent among organotin (IV) compounds. The individual properties of the organotin (IV) and dithiocarbamate moieties in the hybrid complex form a synergy of action that stimulates increased biological activity. Organotin (IV) components have been shown to play a crucial role in cytotoxicity. The biological effects of organotin compounds are believed to be influenced by the number of Sn-C bonds and the number and nature of alkyl or aryl substituents within the organotin structure. Ligands target and react with molecules while preventing unwanted changes in the biomolecules. Organotin (IV) dithiocarbamate compounds have also been shown to have a broad range of cellular, biochemical, and molecular effects, with their toxicity largely determined by their structure. Continuing the investigation of the cytotoxicity of organotin (IV) dithiocarbamates, this mini-review delves into the appropriate method for synthesis and discusses the elemental and spectroscopic analyses and potential cytotoxic effects of these compounds from articles published since 2010.
Radioprotective Effects from Propolis: A Review
Authors: Ibáñez Blanca , Melero Ana , Montoro Alegría , San Onofre Nadia , Soriano Jose M.
Published: August 2023

Propolis is a natural bee-produced substance with antimicrobial, anti-inflammatory, and wound-healing properties, containing some components from the leaves, buds and resins of plants. It has been used for centuries for various health benefits. In this manuscript, our group reviewed the radioprotective effect of propolis using PubMed and Embase, and our review was conducted according to the PRISMA statement. Finally, 27 articles were included in this review, which includes the radioprotective effect of propolis from cell-based studies (n = 8), animal models (n = 14), and human trials (n = 5). Results reflected that the dosage forms of propolis extracted in the scientific literature were ethanolic extracts of propolis, a water-soluble derivate of propolis, or capsules. The efficacy of the radioprotective properties from propolis is extracted from the bibliography, as several compounds of this resinous mixture individually or synergistically are possible candidates that have the radioprotective effect. In fact, studies prior to 2011 lacked a comprehensive characterization of propolis due to the variability in active compounds among different batches of propolis and were limited to analytical techniques. Furthermore, in this manuscript, we have selected studies to include primarily propolis types from Brazil, Croatia, Egypt, European countries, and those commercialized in Spain. They all contained ethanolic extract of propolis (EEP) and were influenced by different dosage forms. EEP showed a significant presence of lipophilic bioactive compounds like flavones, flavonols, and flavanones.
MATH: A Deep Learning Approach in QSAR for Estrogen Receptor Alpha Inhibitors
Authors: Pusparini Rizki Triyani , Krisnadhi Adila Alfa , Firdayani
Published: August 2023

Breast cancer ranks as the second leading cause of death among women, but early screening and self-awareness can help prevent it. Hormone therapy drugs that target estrogen levels offer potential treatments. However, conventional drug discovery entails extensive, costly processes. This study presents a framework for analyzing the quantitative structure–activity relationship (QSAR) of estrogen receptor alpha inhibitors. Our approach utilizes supervised learning, integrating self-attention Transformer and molecular graph information, to predict estrogen receptor alpha inhibitors. We established five classification models for predicting these inhibitors in breast cancer. Among these models, our proposed MATH model achieved remarkable precision, recall, F1 score, and specificity, with values of 0.952, 0.972, 0.960, and 0.922, respectively, alongside an ROC AUC of 0.977. MATH exhibited robust performance, suggesting its potential to assist pharmaceutical and health researchers in identifying candidate compounds for estrogen alpha inhibitors and guiding drug discovery pathways.
Elucidation of Pharmacological Mechanism Underlying the Anti-Alzheimer’s Disease Effects of Evodia rutaecarpa and Discovery of Novel Lead Molecules: An In Silico Study
Authors: Zhang Lulu , Xu Jia , Guo Jiejie , Wang Yun , Wang Qinwen
Published: August 2023

Alzheimer’s disease (AD) is a brain disease with a peculiarity of multiformity and an insidious onset. Multiple-target drugs, especially Chinese traditional medicine, have achieved a measure of success in AD treatment. Evodia rutaecarpa (Juss.) Benth. (Wuzhuyu, WZY, i.e., E. rutaecarpa), a traditional Chinese herb, has been identified as an effective drug to cure migraines. To our surprise, our in silico study showed that rather than migraines, Alzheimer’s disease was the primary disease to which the E. rutaecarpa active compounds were targeted. Correspondingly, a behavioral experiment showed that E. rutaecarpa extract could improve impairments in learning and memory in AD model mice. However, the mechanism underlying the way that E. rutaecarpa compounds target AD is still not clear. For this purpose, we employed methods of pharmacology networking and molecular docking to explore this mechanism. We found that E. rutaecarpa showed significant AD-targeting characteristics, and alkaloids of E. rutaecarpa played the main role in binding to the key nodes of AD. Our research detected that E. rutaecarpa affects the pathologic development of AD through the serotonergic synapse signaling pathway (SLC6A4), hormones (PTGS2, ESR1, AR), anti-neuroinflammation (SRC, TNF, NOS3), transcription regulation (NR3C1), and molecular chaperones (HSP90AA1), especially in the key nodes of PTGS2, AR, SLCA64, and SRC. Graveoline, 5-methoxy-N, N-dimethyltryptamine, dehydroevodiamine, and goshuyuamide II in E. rutaecarpa show stronger binding affinities to these key proteins than currently known preclinical and clinical drugs, showing a great potential to be developed as lead molecules for treating AD.
Computer-Aided Lipase Engineering for Improving Their Stability and Activity in the Food Industry: State of the Art
Authors: Cheng Wenjun , Nian Binbin
Published: August 2023

As some of the most widely used biocatalysts, lipases have exhibited extreme advantages in many processes, such as esterification, amidation, and transesterification reactions, which causes them to be widely used in food industrial production. However, natural lipases have drawbacks in terms of organic solvent resistance, thermostability, selectivity, etc., which limits some of their applications in the field of foods. In this systematic review, the application of lipases in various food processes was summarized. Moreover, the general structure of lipases is discussed in-depth, and the engineering strategies that can be used in lipase engineering are also summarized. The protocols of some classical methods are compared and discussed, which can provide some information about how to choose methods of lipase engineering. Thermostability engineering and solvent tolerance engineering are highlighted in this review, and the basic principles for improving thermostability and solvent tolerance are summarized. In the future, comput er-aided technology should be more emphasized in the investigation of the mechanisms of reactions catalyzed by lipases and guide the engineering of lipases. The engineering of lipase tunnels to improve the diffusion of substrates is also a promising prospect for further enhanced lipase activity and selectivity.
Artificial Small Molecules as Cofactors and Biomacromolecular Building Blocks in Synthetic Biology: Design, Synthesis, Applications, and Challenges
Authors: Liu Fenghua , He Lingling , Dong Sheng , Xuan Jinsong , Cui Qiu , Feng Yingang
Published: August 2023

Enzymes are essential catalysts for various chemical reactions in biological systems and often rely on metal ions or cofactors to stabilize their structure or perform functions. Improving enzyme performance has always been an important direction of protein engineering. In recent years, various artificial small molecules have been successfully used in enzyme engineering. The types of enzymatic reactions and metabolic pathways in cells can be expanded by the incorporation of these artificial small molecules either as cofactors or as building blocks of proteins and nucleic acids, which greatly promotes the development and application of biotechnology. In this review, we summarized research on artificial small molecules including biological metal cluster mimics, coenzyme analogs (mNADs), designer cofactors, non-natural nucleotides (XNAs), and non-natural amino acids (nnAAs), focusing on their design, synthesis, and applications as well as the current challenges in synthetic biology.
Domino Aldol-SNAr-Dehydration Sequence for [3+3] Annulations to Prepare Quinolin-2(1H)-ones and 1,8-Naphthyridin-2(1H)-ones
Authors: Fobi Kwabena , Ametsetor Ebenezer , Bunce Richard A.
Published: August 2023

A domino aldol-SNAr-dehydration [3+3] annulation strategy has been utilized to fuse six-membered cyclic amides onto aromatic substrates. 2-Arylacetamides have been reacted with 2-fluorobenzaldehyde derivatives activated toward SNAr reaction by an electron-withdrawing substituent (NO2, CN, CF3, CO2Me) at C5 to prepare 3,6-disubstituted quinolin-2(1H)-ones. Additionally, 3-substituted 1,8-naphthyridin-2(1H)-ones have been similarly derived from 2-fluoronicotinaldehyde. Fifteen examples are reported, and two possible mechanistic scenarios are presented and discussed.