bluuebunny/crossref_metadata_2025 huggingface dataset:
miR-29b Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells
Authors: Shin Jae-Min , Park Joo-Hoo , Yang Hyun-Woo , Moon Jee Won , Lee Heung-Man , Park Il-Ho
Published: October 2021

Tissue remodeling contributes to ongoing inflammation and refractoriness of chronic rhinosinusitis (CRS). During this process, epithelial-mesenchymal transition (EMT) plays an important role in dysregulated remodeling and both microRNA (miR)-29b and heat shock protein 47 (HSP47) may be engaged in the pathophysiology of CRS. This study aimed to determine the role of miR-29b and HSP47 in modulating transforming growth factor (TGF)-β1-induced EMT and migration in airway epithelial cells. Expression levels of miR-29b, HSP47, E-cadherin, α-smooth muscle actin (α-SMA), vimentin and fibronectin were assessed through real-time PCR, Western blotting, and immunofluorescence staining. Small interfering RNA (siRNA) targeted against miR-29b and HSP47 were transfected to regulate the expression of EMT-related markers. Cell migration was evaluated with wound scratch and transwell migration assay. miR-29b mimic significantly inhibited the expression of HSP47 and TGF-β1-induced EMT-related markers in A549 cells. However, the miR-29b inhibitor more greatly induced the expression of them. HSP47 knockout suppressed TGF-β1-induced EMT marker levels. Functional studies indicated that TGF-β1-induced EMT was regulated by miR-29b and HSP47 in A549 cells. These findings were further verified in primary nasal epithelial cells. miR-29b modulated TGF-β1-induced EMT-related markers and migration via HSP47 expression modulation in A549 and primary nasal epithelial cells. These results suggested the importance of miR-29b and HSP47 in pathologic tissue remodeling progression in CRS.
The Concentration of Organic Acids in Cranberry Juice Modulates the Gut Microbiota in Mice
Authors: Renaud Valentine , Houde Vanessa P. , Pilon Geneviève , Varin Thibault V. , Roblet Cyril , Marette André , Boutin Yvan , Bazinet Laurent
Published: October 2021

A daily consumption of cranberry juice (CJ) is linked to many beneficial health effects due to its richness in polyphenols but could also awake some intestinal discomforts due to its organic acid content and possibly lead to intestinal inflammation. Additionally, the impact of such a juice on the gut microbiota is still unknown. Thus, this study aimed to determine the impacts of a daily consumption of CJ and its successive deacidification on the intestinal inflammation and on the gut microbiota in mice. Four deacidified CJs (DCJs) (deacidification rates of 0, 40, 60, and 80%) were produced by electrodialysis with bipolar membrane (EDBM) and administered to C57BL/6J mice for four weeks, while the diet (CHOW) and the water were ad libitum. Different parameters were measured to determine intestinal inflammation when the gut microbiota was profiled. Treatment with a 0% DCJ did not induce intestinal inflammation but increased the gut microbiota diversity and induced a modulation of its functions in comparison with control (water). The effect of the removal of the organic acid content of CJ on the decrease of intestinal inflammation could not be observed. However, deacidification by EDBM of CJ induced an additional increase, in comparison with a 0% DCJ, in the Lachnospiraceae family which have beneficial effects and functions associated with protection of the intestine: the lower the organic acid content, the more bacteria of the Lachnospiraceae family and functions having a positive impact on the gut microbiota.
Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss
Authors: Verdoodt Dorien , Peeleman Noa , Szewczyk Krystyna , Van Camp Guy , Ponsaerts Peter , Van Rompaey Vincent
Published: October 2021

Several studies have shown that type IV fibrocytes, located in the spiral ligament, degenerate first after noise exposure. Interestingly, this is the region where Coch expression is most abundant. As it is suggested that cochlin plays a role in our innate immune system, our goal is to investigate hearing thresholds and inner ear inflammation after noise exposure in Coch knockout (Coch−/−) mice compared to Coch wildtype (Coch+/+) mice. Animals were randomly allocated to a noise exposure group and a control group. Vestibular and auditory testing was performed at 48 h and one week after noise exposure. Whole mount staining and cryosectioning of the cochlea was performed in order to investigate hair cells, spiral ganglion neurons, inner ear inflammation, Coch expression and fibrocyte degeneration. Hearing assessment revealed that Coch+/+ mice had significantly larger threshold shifts than Coch−/− mice after noise exposure. We were unable to identify any differences in hair cells, neurons, fibrocytes and influx of macrophages in the inner ear between both groups. Interestingly, Coch expression was significantly lower in the group exposed to noise. Our results indicate that the absence of Coch has a protective influence on hearing thresholds after noise exposure, but this is not related to reduced inner ear inflammation in the knockout.
Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature
Authors: Battistella Roberta , Kritsilis Marios , Matuskova Hana , Haswell Douglas , Cheng Anne Xiaoan , Meissner Anja , Nedergaard Maiken , Lundgaard Iben
Published: October 2021

The vascular system is vital for all tissues and the interest in its visualization spans many fields. A number of different plant-derived lectins are used for detection of vasculature; however, studies performing direct comparison of the labeling efficacy of different lectins and techniques are lacking. In this study, we compared the labeling efficacy of three lectins: Griffonia simplicifolia isolectin B4 (IB4); wheat germ agglutinin (WGA), and Lycopersicon esculentum agglutinin (LEA). The LEA lectin was identified as being far superior to the IB4 and WGA lectins in histological labeling of blood vessels in brain sections. A similar signal-to-noise ratio was achieved with high concentrations of the WGA lectin injected during intracardial perfusion. Lectins were also suitable for labeling vasculature in other tissues, including spinal cord, dura mater, heart, skeletal muscle, kidney, and liver tissues. In uninjured tissues, the LEA lectin was as accurate as the Tie2–eGFP reporter mice and GLUT-1 immunohistochemistry for labeling the cerebral vasculature, validating its specificity and sensitivity. However, in pathological situations, e.g., in stroke, the sensitivity of the LEA lectin decreases dramatically, limiting its applicability in such studies. This work can be used for selecting the type of lectin and labeling method for various tissues.
Revealing the Molecular Mechanisms of Alzheimer’s Disease Based on Network Analysis
Authors: Bayraktar Abdulahad , Lam Simon , Altay Ozlem , Li Xiangyu , Yuan Meng , Zhang Cheng , Arif Muhammad , Turkez Hasan , Uhlén Mathias , Shoaie Saeed , Mardinoglu Adil
Published: October 2021

The complex pathology of Alzheimer’s disease (AD) emphasises the need for comprehensive modelling of the disease, which may lead to the development of efficient treatment strategies. To address this challenge, we analysed transcriptome data of post-mortem human brain samples of healthy elders and individuals with late-onset AD from the Religious Orders Study and Rush Memory and Aging Project (ROSMAP) and Mayo Clinic (MayoRNAseq) studies in the AMP-AD consortium. In this context, we conducted several bioinformatics and systems medicine analyses including the construction of AD-specific co-expression networks and genome-scale metabolic modelling of the brain in AD patients to identify key genes, metabolites and pathways involved in the progression of AD. We identified AMIGO1 and GRPRASP2 as examples of commonly altered marker genes in AD patients. Moreover, we found alterations in energy metabolism, represented by reduced oxidative phosphorylation and ATPase activity, as well as the depletion of hexanoyl-CoA, pentanoyl-CoA, (2E)-hexenoyl-CoA and numerous other unsaturated fatty acids in the brain. We also observed that neuroprotective metabolites (e.g., vitamins, retinoids and unsaturated fatty acids) tend to be depleted in the AD brain, while neurotoxic metabolites (e.g., β-alanine, bilirubin) were more abundant. In summary, we systematically revealed the key genes and pathways related to the progression of AD, gained insight into the crucial mechanisms of AD and identified some possible targets that could be used in the treatment of AD.
A Brief History and Future Prospects of CEST MRI in Clinical Non-Brain Tumor Imaging
Authors: Gao Tianxin , Zou Chuyue , Li Yifan , Jiang Zhenqi , Tang Xiaoying , Song Xiaolei
Published: October 2021

Chemical exchange saturation transfer (CEST) MRI is a promising molecular imaging tool which allows the specific detection of metabolites that contain exchangeable amide, amine, and hydroxyl protons. Decades of development have progressed CEST imaging from an initial concept to a clinical imaging tool that is used to assess tumor metabolism. The first translation efforts involved brain imaging, but this has now progressed to imaging other body tissues. In this review, we summarize studies using CEST MRI to image a range of tumor types, including breast cancer, pelvic tumors, digestive tumors, and lung cancer. Approximately two thirds of the published studies involved breast or pelvic tumors which are sites that are less affected by body motion. Most studies conclude that CEST shows good potential for the differentiation of malignant from benign lesions with a number of reports now extending to compare different histological classifications along with the effects of anti-cancer treatments. Despite CEST being a unique ‘label-free’ approach with a higher sensitivity than MR spectroscopy, there are still some obstacles for implementing its clinical use. Future research is now focused on overcoming these challenges. Vigorous ongoing development and further clinical trials are expected to see CEST technology become more widely implemented as a mainstream imaging technology.
Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
Authors: Janoniene Agne , Mazutis Linas , Matulis Daumantas , Petrikaite Vilma
Published: October 2021

Protein Carbonic Anhydrase IX (CA IX), which is expressed in various hypoxic solid tumors in order to maintain proper pH, is also related to cancer cell adhesion, invasion, and metastasis processes. Here, we investigated whether CA IX inhibition by a highly CA IX selective agent benzenesulfonamide VD11-4-2 triggers changes in individual cell motility. We seeded breast cancer cells on an extracellular matrix-coated glass-bottomed dish and in a microfluidic device with a gradient flow of epidermal growth factor (EGF), tracked individual cell movement, calculated their migration speeds, and/or followed movement direction. Our results showed that the inhibitor VD11-4-2 decreased the speed of CA IX positive breast cancer cells by 20–26% while not affecting non-cancerous cell migration. The inhibitor suppressed the cell migration velocity increment and hindered cells from reaching their maximum speed. VD11-4-2 also reduced CA IX, expressing cell movement towards the growth factor as a chemoattractant. Such a single cell-based migration assay enabled the comprehensive investigation of the cell motility and revealed that VD11-4-2 shows the ability to suppress breast cancer cell migration at a lower concentration than previously tested CA IX inhibitors.
Current Concepts of Psoriasis Immunopathogenesis
Authors: Vičić Marijana , Kaštelan Marija , Brajac Ines , Sotošek Vlatka , Massari Larisa Prpić
Published: October 2021

Psoriasis is a recurrent, chronic, immune-mediated, systemic inflammatory disease of the skin, joints, and other organic systems. After atopic dermatitis, chronic stationary psoriasis is the most common inflammatory skin disease, affecting an average of 2–4% of the world’s population. The disease carries a significant burden due to its numerous comorbidities and the major impact on patients’ social and emotional aspects of life. According to current knowledge, psoriasis is a multifactorial disease that occurs in genetically predisposed individuals under various environmental factors, which trigger an immune response disorder with a series of complex inflammatory cascades. The disease is initiated and maintained by mutual interaction of the innate and adaptive immune cells, primarily dendritic cells, T lymphocytes, and keratinocytes, whose leading role alternates at different stages of the disease, consisting mainly in the IL-23/Th17 pathway. Inflammatory events result in consequent epidermal and dermal changes and evolution of the characteristic psoriatic phenotype, respectively. This paper aims to present a comprehensive overview of current knowledge on psoriasis genetic and environmental etiological factors, immunopathogenesis, and the leading cellular and cytokine participants in the inflammatory pathways of this disease.
Recent Advances in Understanding the Role of TIGIT+ Follicular Helper T Cells in IgG4-Related Disease
Authors: Akiyama Mitsuhiro , Kaneko Yuko
Published: October 2021

IgG4-related disease (IgG4-RD) is a fibro-inflammatory disease characterized by elevated serum IgG4 levels and massive infiltration of IgG4+plasma cells. Although storiform fibrosis, obliterative phlebitis and IgG4+plasma cell infiltration are well described pathological features in this disease, the excessive formation of tertiary lymphoid organs (TLOs), particularly in the early phase of the disease lesions, has gained much attention. TLOs of IgG4-RD are orchestrated by specific immune cell subsets including follicular helper T cells (Tfh), CD20+ B cells, and CD21+ follicular dendritic cells (FDCs). Tfh is the key player of this disease because recent studies have suggested the pathological role of this immune cell subset in formation of TLOs, helping IgG4+plasma cell differentiation, inducing storiform fibrosis by secreting interleukin-4, and activating cytotoxic T cells by secreting interleukin-21. We have recently identified a new Tfh subset which expresses T cell immunoreceptor with immunoglobulin and ITIM domain (TIGIT). TIGIT+Tfh efficiently produces interleukin-21 through OX40 signal, and the increase in peripheral TIGIT+Tfh cells reflects disease activity in IgG4-RD. TIGIT is important to mediate the retention and positioning of TIGIT+Tfh within TLOs through interaction with CD155 expressed on CD21+ FDCs. In this review, we summarize and discuss recent progress in understanding the pathogenesis of IgG4-RD, focusing on TIGIT+Tfh.
Robotic Psychology: A PRISMA Systematic Review on Social-Robot-Based Interventions in Psychological Domains
Authors: Duradoni Mirko , Colombini Giulia , Russo Paola Andrea , Guazzini Andrea
Published: October 2021

Current technological advancements have allowed robots to be successfully employed in the healthcare sector. However, the recently acquired ability of social robots to process social information and act according to it has potentially made them very well suited to support or conduct psychological interventions. The present paper carried out a systematic review of the available literature regarding social-robot-based interventions in psychological domains using preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. The inclusion criteria were: (i) publication date until 2020; (ii) being an empirical study, master thesis, or project report; (iii) written in English or Italian languages (the two languages spoken by the authors); (iv) published in a scholarly peer-reviewed journal or conference proceedings, or were Ph.D. or master’s theses; and (v) assessed “social robot”-based intervention in psychological domains. Overall, the review showed that three main areas may benefit from social-robot-based interventions: social skills, mood, and wellbeing (e.g., stress and anxiety levels). Interestingly, social robots seemed to have a performance comparable to, and sometimes even better than, human operators. The main, but not exclusive, target of robot-based interventions in the psychological field was children with autism spectrum disorder (ASD). As evidence is, however, still limited and in an embryonic state, deeper investigations are needed to assess the full potential of social robots for the purposes of psychological intervention. This is relevant, considering the role that social robots could have in overcoming barriers to access psychological assessment and therapies.
Sphingomonas and Phenylobacterium as Major Microbiota in Thymic Epithelial Tumors
Authors: Higuchi Rumi , Goto Taichiro , Hirotsu Yosuke , Otake Sotaro , Oyama Toshio , Amemiya Kenji , Ohyama Hiroshi , Mochizuki Hitoshi , Omata Masao
Published: October 2021

The microbiota has been reported to be closely associated with carcinogenesis and cancer progression. However, its involvement in the pathology of thymoma remains unknown. In this study, we aimed to identify thymoma-specific microbiota using resected thymoma samples. Nineteen thymoma tissue samples were analyzed through polymerase chain reaction amplification and 16S rRNA gene sequencing. The subjects were grouped according to histology, driver mutation status in the GTF2I gene, PD-L1 status, and smoking habits. To identify the taxa composition of each sample, the operational taxonomic units (OTUs) were classified on the effective tags with 97% identity. The Shannon Index of the 97% identity OTUs was calculated to evaluate the alpha diversity. The linear discriminant analysis effect size (LEfSe) method was used to compare the relative abundances of all the bacterial taxa. We identified 107 OTUs in the tumor tissues, which were classified into 26 genera. Sphingomonas and Phenylobacterium were identified as abundant genera in almost all the samples. No significant difference was determined in the alpha diversity within these groups; however, type A thymoma tended to exhibit a higher bacterial diversity than type B thymoma. Through the LEfSe analysis, we identified the following differentially abundant taxa: Bacilli, Firmicutes, and Lactobacillales in type A thymoma; Proteobacteria in type B thymoma; Gammaproteobacteria in tumors harboring the GTF2I mutation; and Alphaproteobacteria in tumors without the GTF2I mutation. In conclusion, Sphingomonas and Phenylobacterium were identified as dominant genera in thymic epithelial tumors. These genera appear to comprise the thymoma-specific microbiota.
Short Term Caloric Restriction and Biofeedback Enhance Psychological Wellbeing and Reduce Overweight in Healthy Women
Authors: Kautzky Alexander , Heneis Kathrin , Stengg Karin , Fröhlich Sabine , Kautzky-Willer Alexandra
Published: October 2021

Obesity is highly prevalent, causing substantial cardiovascular and mental health morbidity. Women show increased risk for mental health disorders, that is multiplied in obesity and related to cellular and psychological stress that can be targeted by non-pharmacological interventions. A total of 43 women underwent two weeks of caloric restriction, half of which also received 7 h of individualized clinical psychological intervention including psychoeducation, mindfulness, and heart-rate-variability biofeedback. Effects on body mass index (BMI), fatty liver index (FLI), bioimpedance measures, serum parameters, perceived stress (PSS), burn-out susceptibility (burn out diagnostic inventory) and dimensional psychiatric symptom load (brief symptom inventory, BSI) were analyzed with linear mixed effects models. Caloric restriction led to a reduction in BMI, body fat and FLI, decreased serum concentrations of leptin, PSS score, BSI dimensions and global severity index (all p ≤ 0.0001, withstanding Bonferroni–Holm correction). Benefits of add-on biofeedback were observed for BMI reduction (p = 0.041). Caloric restriction was effective in ameliorating both psychological wellbeing and metabolic functions following a BMI reduction. Biofeedback boosted effects on BMI reduction and the combinative therapy may be protective against common progression to mental health and cardiovascular disorders in overweight women while comparing favorably to pharmacological interventions in terms of side-effects and acceptability.
Injection Moulding of Multipolar Polymer-Bonded Magnets into Soft Magnetic Inserts for Rotors in Reluctance Motors
Authors: Rösel Uta , Drummer Dietmar
Published: October 2021

Due to lower magnetic properties of polymer-bonded magnets compared to sintered magnets, a complete redesign of the multipolar soft magnetic flux barriers in rotors with alignment guidelines was carried out to eliminate the frequently used rare-earth magnets, causing a new influence of the outer magnetic field on the cavity by using soft magnetic inserts. Within this new process, the main influencing factors on the magnetic flux density such as filler content, tool temperature, holding pressure and injection velocity were analysed and correlated. The studies were based on the compound of Polyamide 12 and up to a 60 vol.-% of the hard magnetic filler, strontium ferrite. Based on the study, the injection moulding of multipolar-bonded magnets into soft magnetic inserts for rotors and, in turn, into complex geometries can be optimized in terms of the orientation of the filler, the microstructure and the magnetic flux density. The investigations show no significant influence of the process parameters known from the literature such as the mass temperature Tm, which affects the magnetic flux density, as well as the orientation and the microstructure similar to tool temperature Tt, but is less efficient. The main influencing factors identified during the investigations are the tool temperature Tt, the injection velocity vin and the holding pressure ph. As known influencing factors are only based on simple geometries such as ring structures or plates, new factors were determined for complex rotor geometries.
Analyzing the Effect of Weak External Transcranial Magnetic Stimulation on the Primary Dominant Frequencies of Alzheimer Patients Brain by Using MEG Recordings
Authors: Anninos Photios , Adamopoulos Adam , Anninou Nikolia , Tsagas Nikolaos
Published: October 2021

Backround and Objectives: Alternative, non-invasive, and non-pharmaceutical options are gaining place in the battle of Alzheimer’s Disease treatment control. Lately, the magnetic stimulation of the brain is the most prevalent technique with encouraging results. The aim of this study is to establish any possible change on the Primary Dominant Frequencies (PDF) (range 2–7 Hz) of the affected brain regions in Alzheimer Disease (AD) patients after applying extremely weak Transcranial Magnetic Stimulation. Materials and Methods: For this purpose, all AD patients were scanned with the use of MagnetoEncephaloGraphy (MEG) recordings through a whole-head 122–channel MEG system. Results: Our results exerted statistically significant PDF changes due to weak TMS accompanied by rabid attenuation of clinical symptoms. Conclusion: Thus, this is the first time that a positive therapeutic effect is being demonstrated even at pico-Tesla range magnetic fields in a small clinical group of studies for AD.
Impact of Membrane Phosphoric Acid Doping Level on Transport Phenomena and Performance in High Temperature PEM Fuel Cells
Authors: Li Shian , Peng Chengdong , Shen Qiuwan , Wang Chongyang , Cheng Yuanzhe , Yang Guogang
Published: October 2021

In this work, a three-dimensional mathematical model including the fluid flow, heat transfer, mass transfer, and charge transfer incorporating electrochemical reactions was developed and applied to investigate the transport phenomena and performance in high-temperature proton exchange membrane fuel cells (HT-PEMFCs) with a membrane phosphoric acid doping level of 5, 7, 9, 11. The cell performance is evaluated and compared in terms of the polarization curve. The distributions of temperature, oxygen mass fraction, water mass fraction, proton conductivity, and local current density of four cases are given and compared in detail. Results show that the overall performance and local transport characteristics are significantly affected by the membrane phosphoric acid doping level.
Effect of Aluminum Ion Irradiation on Chemical and Phase Composition of Surface Layers of Rolled AISI 321 Stainless Steel
Authors: Bykov Pavel V. , Bayankin Vladimir Y. , Tcherdyntsev Victor V. , Vorob’ev Vasiliy L. , Pechina Elena A. , Sviridova Tatyana A. , Shushkov Andrey A. , Chukavin Andrey I. , Alexandrova Svetlana S.
Published: October 2021

Commercial rolled AISI 321 stainless steel samples were irradiated with Al+ ions with an energy of 80 keV and fluence of 1017 ion/cm2. The effect of Al implantation on the chemical and phase composition of the steel surface layer was studied by X-ray electron spectroscopy and grazing beam mode of X-ray diffraction analysis. A thin surface layer down to a depth of 30 nm after Al+ ions implantation consists mainly of metal oxides. In the near-surface layers of 5 nm in depth, a noticeable depletion in chromium and nickel was observed. A surface layer (up to 0.5 µm) of non-irradiated steel, in addition to the f.c.c. austenite γ-phase, consists of up to 20 vol% of the b.c.c. α′-phase, which formed at rolling as a result of mechanical deformation. Al implantation results in the significant increase in the α′-phase amount in the surface layer at a depth up to 2 µm. It is indicated that the observed γ → α′ transformation at ion irradiation proceeds predominantly as a result of the effect of post-cascade shock waves, but not as a result of the surface layer chemical composition changes.
Effects of Fe/Si Stoichiometry on Formation of Fe3Si/FeSi-Al2O3 Composites by Aluminothermic Combustion Synthesis
Authors: Yeh Chun-Liang , Chen Kuan-Ting , Shieh Tzong-Hann
Published: October 2021

Aluminothermic combustion synthesis was conducted with Fe2O3–Al–Fe–Si reaction systems under Fe/Si stoichiometry from Fe-20 to Fe-50 at. % Si to investigate the formation Fe3Si/FeSi–Al2O3 composites. The solid-state combustion was sufficiently exothermic to sustain the overall reaction in the mode of self-propagating high-temperature synthesis (SHS). Dependence of iron silicide phases formed from SHS on Fe/Si stoichiometry was examined. Experimental evidence indicated that combustion exothermicity and flame-front velocity were affected by the Si percentage. According to the X-ray diffraction (XRD) analysis, Fe3Si–Al2O3 composites were synthesized from the reaction systems with Fe-20 and Fe-25 at.% Si. The increase of Si content led to the formation of both Fe3Si and FeSi in the final products of Fe-33.3 and Fe-40 at.% Si reaction systems, and the content of FeSi increased with Si percentage. Further increase of Si to Fe-50 at.% Si produced the FeSi–Al2O3 composite. Scanning electron microscopy (SEM) images revealed that the fracture surface morphology of the products featured micron-sized and nearly spherical Fe3Si and FeSi particles distributing over the dense and connecting substrate formed by Al2O3.
Chalcogen Bond Involving Zinc(II)/Cadmium(II) Carbonate and Its Enhancement by Spodium Bond
Authors: Liu Na , Xie Xiaoying , Li Qingzhong
Published: October 2021

Carbonate MCO3 (M = Zn, Cd) can act as both Lewis acid and base to engage in a spodium bond with nitrogen-containing bases (HCN, NHCH2, and NH3) and a chalcogen bond with SeHX (X = F, Cl, OH, OCH3, NH2, and NHCH3), respectively. There is also a weak hydrogen bond in the chalcogen-bonded dyads. Both chalcogen and hydrogen bonds become stronger in the order of F > Cl > OH > OCH3 > NH2 > NHCH3. The chalcogen-bonded dyads are stabilized by a combination of electrostatic and charge transfer interactions. The interaction energy of chalcogen-bonded dyad is less than −10 kcal/mol at most cases. Furthermore, the chalcogen bond can be strengthened through coexistence with a spodium bond in N-base-MCO3-SeHX. The enhancement of chalcogen bond is primarily attributed to the charge transfer interaction. Additionally, the spodium bond is also enhanced by the chalcogen bond although the corresponding enhancing effect is small.
Evaluation of the Antimicrobial Effect of Thymoquinone against Different Dental Pathogens: An In Vitro Study
Authors: Al-Khalifa Khalifa S. , AlSheikh Rasha , Al-Hariri Moahmmed T. , El-Sayyad Hosam , Alqurashi Maher S. , Ali Saqib , Bugshan Amr S.
Published: October 2021

This study aimed to evaluate the antimicrobial effect of Thymoquinone (TQ) on four different oral microorganisms. Minimum Bactericidal Concentration (MBC), Minimum Inhibition Concentration (MIC), Broth microdilution, and Well diffusion tests were used to determine the optimum antimicrobial concentrations of TQ against Streptococcus salivarius, Streptococcus oralis, Streptococcus mutans, and Staphylococcus aureus over 1, 3, 6, 12 and 24 h. Chlorhexidine 0.12% was selected as a positive control. The inhibitory effect of TQ on bacterial growth was most noticeable with S. salivarius, while the least affected was S. aureus. TQ’s MBC and MIC for S. oralis and S. aureus were comparable 2 mg/mL and 3 mg/mL, respectively. S. salivarius was most resistant to TQ and displayed a value of 5 mg/mL and 4 mg/mL for MIC and MBC, respectively. The viable count of different strains after exposure to TQ’s MBC values was most noticeable with S. aureus followed by S. oralis and S. mutans, while S. salivarius was least affected. This study emphasized the promising antimicrobial effect of TQ against the four main oral microorganisms. It has a potential preventive effect against dental caries as well as other oral diseases.
Use of Polyhedral Oligomeric Silsesquioxane (POSS) in Drug Delivery, Photodynamic Therapy and Bioimaging
Authors: Loman-Cortes Paula , Binte Huq Tamanna , Vivero-Escoto Juan L.
Published: October 2021

Polyhedral oligomeric silsesquioxanes (POSS) have attracted considerable attention in the design of novel organic-inorganic hybrid materials with high performance capabilities. Features such as their well-defined nanoscale structure, chemical tunability, and biocompatibility make POSS an ideal building block to fabricate hybrid materials for biomedical applications. This review highlights recent advances in the application of POSS-based hybrid materials, with particular emphasis on drug delivery, photodynamic therapy and bioimaging. The design and synthesis of POSS-based materials is described, along with the current methods for controlling their chemical functionalization for biomedical applications. We summarize the advantages of using POSS for several drug delivery applications. We also describe the current progress on using POSS-based materials to improve photodynamic therapies. The use of POSS for delivery of contrast agents or as a passivating agent for nanoprobes is also summarized. We envision that POSS-based hybrid materials have great potential for a variety of biomedical applications including drug delivery, photodynamic therapy and bioimaging.
Elemental Selenium Enriched Nanofiber Production
Authors: Badgar Khandsuren , Prokisch József
Published: October 2021

This study aimed to produce electrospun nanofibers from a polyvinyl butyral polymer (PVB) solution enriched with red and grey selenium nanoparticles. Scanning electron microscopic analysis was used to observe the samples, evaluate the fiber diameters, and reveal eventual artifacts in the nanofibrous structure. Average fiber diameter is determined by manually measuring the diameters of randomly selected fibers on scanning electron microscopic (SEM) images. The obtained nanofibers are amorphous with a diameter of approximately 500 nm, a specific surface area of approx. 8 m2 g−1, and 5093 km cm−3 length. If the red and grey selenium nanoparticles were produced in powder form and suspended to the ethanolic solution of PVB then they were located inside and outside the fiber. When selenium nanoparticles were synthesized in the PVB solution, then they were located only inside the fiber. These nanofiber sheets enriched with selenium nanoparticles could be a good candidate for high-efficiency filter materials and medical applications.
Molecular Mechanisms Associated with Clustered Lesion-Induced Impairment of 8-oxoG Recognition by the Human Glycosylase OGG1
Authors: Jiang Tao , Monari Antonio , Dumont Elise , Bignon Emmanuelle
Published: October 2021

The 8-oxo-7,8-dihydroguanine, referred to as 8-oxoG, is a highly mutagenic DNA lesion that can provoke the appearance of mismatches if it escapes the DNA Damage Response. The specific recognition of its structural signature by the hOGG1 glycosylase is the first step along the Base Excision Repair pathway, which ensures the integrity of the genome by preventing the emergence of mutations. 8-oxoG formation, structural features, and repair have been matters of extensive research; more recently, this active field of research expended to the more complicated case of 8-oxoG within clustered lesions. Indeed, the presence of a second lesion within 1 or 2 helix turns can dramatically impact the repair yields of 8-oxoG by glycosylases. In this work, we use μs-range molecular dynamics simulations and machine-learning-based postanalysis to explore the molecular mechanisms associated with the recognition of 8-oxoG by hOGG1 when embedded in a multiple-lesion site with a mismatch in 5′ or 3′. We delineate the stiffening of the DNA–protein interactions upon the presence of the mismatches, and rationalize the much lower repair yields reported with a 5′ mismatch by describing the perturbation of 8-oxoG structural features upon addition of an adjacent lesion.
Polish Varieties of Industrial Hemp and Their Utilisation in the Efficient Production of Lignocellulosic Ethanol
Authors: Wawro Aleksandra , Batog Jolanta , Gieparda Weronika
Published: October 2021

Nowadays, more and more attention is paid to the development and the intensification of the use of renewable energy sources. Hemp might be an alternative plant for bioenergy production. In this paper, four varieties of Polish industrial hemp (Białobrzeskie, Tygra, Henola, and Rajan) were investigated in order to determine which of them are the most advantageous raw materials for the effective production of bioethanol. At the beginning, physical and chemical pretreatment of hemp biomass was carried out. It was found that the most effective is the alkaline treatment with 2% NaOH, and the biomasses of the two varieties were selected for next stages of research: Tygra and Rajan. Hemp biomass before and after pretreatment was analyzed by FTIR and SEM, which confirmed the effectiveness of the pretreatment. Next, an enzymatic hydrolysis process was carried out on the previously selected parameters using the response surface methodology. Subsequently, the two approaches were analyzed: separated hydrolysis and fermentation (SHF) and a simultaneous saccharification and fermentation (SSF) process. For Tygra biomass in the SHF process, the ethanol concentration was 10.5 g∙L−1 (3.04 m3·ha−1), and for Rajan biomass at the SSF process, the ethanol concentration was 7.5 g∙L−1 (2.23 m3·ha−1). In conclusion, the biomass of Polish varieties of hemp, i.e., Tygra and Rajan, was found to be an interesting and promising raw material for bioethanol production.
Smartphone and the Self: Experimental Investigation of Self-Incorporation of and Attachment to Smartphones
Authors: Gertz Marlene , Schütz-Bosbach Simone , Diefenbach Sarah
Published: October 2021

Smartphones are a constant companion in everyday life. Interacting with a smartphone calls for a multimodal input and often leads to a multisensory output. Combining research in human-computer interaction (HCI) and psychology, the present research explored the idea that a smartphone is more than a smart object but represents an object to which people feel emotionally attached to and which is even perceived as a part or an extension of a person’s self. To this end, we used an established rubber hand illusion paradigm to experimentally induce body ownership experiences in young adults (n = 76) in a 4-level mixed-design study. Our results revealed that in contrast to a neutral control object participants indeed felt attached to a smartphone, perceived it as a part of themselves and felt the need to interact with the device. This was specifically pronounced when hedonic characteristics were evaluated as high and when its usage for social communication was highlighted during the experiment. Psychological mechanisms of the incorporation of technologies are discussed and connected to positive and negative effects of smartphone usage on human behavior, its implications for technology design and marketing.
Sox2 Ablation in the Suprachiasmatic Nucleus Perturbs Anxiety- and Depressive-like Behaviors
Authors: Boehler Nicholas A. , Fung Samuel W. , Hegazi Sara , Cheng Arthur H. , Cheng Hai-Ying Mary
Published: October 2021

Mood disorders negatively impact the lives of hundreds of millions of individuals worldwide every year, yet the precise molecular mechanisms by which they manifest remain elusive. Circadian dysregulation is one avenue by which mood disorders are thought to arise. SOX2 is a transcription factor that is highly expressed in the murine suprachiasmatic nucleus (SCN), the circadian master clock, and has been recently found to be an important regulator of Per2, a core component of the molecular clock. Genetic ablation of the Sox2 gene in GABAergic neurons selectively impacts SCN neurons, as they are one of very few, if not the only, GABAergic populations that express Sox2. Here, we show that GABAergic-restricted ablation of Sox2 results in anxio-depressive-like phenotypes in mice as observed in the elevated plus maze, forced swim test, tail suspension test, and sucrose preference test. We further observe a reduction in basal and/or forced swim-induced c-Fos expression, a marker of neuronal activation, in the nucleus incertus, arcuate nucleus, and dentate gyrus of Sox2 conditional knockout (cKO) mice. Given the restricted disruption of SOX2 expression in the SCN of Sox2 cKO mice, we propose that their mood-associated phenotypes are the consequence of a dysregulated central clock that is unable to communicate appropriately timed signals to other brain nuclei that regulate affective behaviors.
Digital Food Retail: Public Health Opportunities
Authors: Fernandez Melissa Anne , Raine Kim Denise
Published: October 2021

For over two decades, digital food retail services have been emerging alongside advances in mobile technology and improved access to wi-fi. Digitalization has driven changes within the food environment, complicating an already complex system that influences food-related behaviors and eating practices. Digital food retail services support an infrastructure that enhances commercial food systems by extending access to and availability of highly processed foods, further escalating poor dietary intakes. However, digital food retail services are heterogeneous–food delivery apps, online groceries, and meal kits–and can be feasibly adapted to nutrition interventions and personalized to individual needs. Although sparse, new evidence indicates great potential for digital food retail services to address food insecurity in urban areas and to support healthy eating by making it easier to select, plan, and prepare meals. Digital food retail services are a product of the digital transformation that reflect consumers’ constant need for convenience, which must be addressed in future research and interventions. This paper will discuss public health opportunities that are emerging from the global uptake of digital food retail services, with a focus on online groceries, food delivery apps, and meal kits.
Adjustments in Food Choices and Physical Activity during Lockdown by Flemish Adults
Authors: Mertens Evelien , Deriemaeker Peter , Van Beneden Katrien
Published: October 2021

Background: On Wednesday 18/03/2020 Belgium was placed in lockdown in order to curb the spread of COVID-19. Lockdown can lead to loneliness, boredom, anger, anxiety and depression, which in turn have an influence on food choices and physical activity (PA). This study aims to map the adjustments in food choices and PA by Flemish adults during lockdown. Methods: Chi square tests were performed to investigate the relationship between adjustments in food choices, PA and demographic variables. Results: A total of 1.129 respondents filled in the online questionnaire, aged between 18 and 81 years. The healthiest food choices were made by respondents living alone during lockdown, whilst people cohabiting with others increased their PA significantly. Moreover, the dietary adjustments of adults living with children evolved more favourably to healthier choices then those cohousing with other adults. However, respondents living with other adults showed a more favourable pattern regarding adjustments in PA. The strongest increase in sedentary behaviour was observed in students. Conclusions: This study shows the impact of lockdown on both PA and food choices, where healthier adjustments were observed in PA and respondents were prone to consume unhealthier food.
Gastrointestinal Hormones in Healthy Adults: Reliability of Repeated Assessments and Interrelations with Eating Habits and Physical Activity
Authors: Wortha Silke M. , Wüsten Katharina A. , Witte Veronica A. , Bössel Nicole , Keßler Wolfram , Vogelgesang Antje , Flöel Agnes
Published: October 2021

Background: Gastrointestinal hormones (GIHs) are crucial for the regulation of a variety of physiological functions and have been linked to hunger, satiety, and appetite control. Thus, they might constitute meaningful biomarkers in longitudinal and interventional studies on eating behavior and body weight control. However, little is known about the physiological levels of GIHs, their intra-individual stability over time, and their interaction with other metabolic and lifestyle-related parameters. Therefore, the aim of this pilot study is to investigate the intra-individual stability of GIHs in normal-weight adults over time. Methods: Plasma concentrations of ghrelin, leptin, GLP-1 (glucagon-like-peptide), and PP (pancreatic polypeptide) were assessed by enzyme-linked immunosorbent assay (ELISA) in 17 normal-weight, healthy adults in a longitudinal design at baseline and at follow-up six months later. The reliability of the measurements was estimated using intra-class correlation (ICC). In a second step, we considered the stability of GIH levels after controlling for changes in blood glucose and hemoglobin A1 (HbA1c) as well as self-reported physical activity and dietary habits. Results: We found excellent reliability for ghrelin, good reliability for GLP1 and PP, and moderate reliability for leptin. After considering glucose, HbA1c, physical activity, and dietary habits as co-variates, the reliability of ghrelin, GLP1, and PP did not change significantly; the reliability of leptin changed to poor reliability. Conclusions: The GIHs ghrelin, GLP1, and PP demonstrated good to excellent test–retest reliability in healthy individuals, a finding that was not modified after adjusting for glucose control, physical activity, or dietary habits. Leptin showed only moderate to poor reliability, which might be linked to weight fluctuations, albeit small, between baseline and follow-up assessment in our study sample. Together, these findings support that ghrelin, GLP1, and PP might be further examined as biomarkers in studies on weight control, with GLP1 and PP serving as anorexic markers and ghrelin as an orexigenic marker. Additional reliability studies in obese individuals are necessary to verify or refute our findings for this cohort.
Two of a Kind? Mapping the Psychopathological Space between Obesity with and without Binge Eating Disorder
Authors: Sommer Laura Marie , Halbeisen Georg , Erim Yesim , Paslakis Georgios
Published: October 2021

(1) Background: Obesity (OB) is a frequent co-morbidity in Binge Eating Disorder (BED), suggesting that both conditions share phenotypical features along a spectrum of eating-related behaviors. However, the evidence is inconsistent. This study aimed to comprehensively compare OB-BED patients against OB individuals without BED and healthy, normal-weight controls in general psychopathological features, eating-related phenotypes, and early life experiences. (2) Methods: OB-BED patients (n = 37), OB individuals (n = 50), and controls (n = 44) completed a battery of standardized questionnaires. Responses were analyzed using univariate comparisons and dimensionality reduction techniques (linear discriminant analysis, LDA). (3) Results: OB-BED patients showed the highest scores across assessments (e.g., depression, emotional and stress eating, food cravings, food addiction). OB-BED patients did not differ from OB individuals in terms of childhood traumatization or attachment styles. The LDA revealed a two-dimensional solution that distinguished controls from OB and OB-BED in terms of increasing problematic eating behaviors and attitudes, depression, and childhood adversities, as well as OB-BED from OB groups in terms of emotional eating tendencies and self-regulation impairments. (4) Conclusions: Findings support the idea of a shared spectrum of eating-related disorders but also highlight important distinctions relevant to identifying and treating BED in obese patients.
Etiology of Anemia in Older Mexican Adults: The Role of Hepcidin, Vitamin A and Vitamin D
Authors: De la Cruz-Góngora Vanessa , Salinas-Rodríguez Aarón , Flores-Aldana Mario , Villalpando Salvador
Published: October 2021

Anemia in older adults is a growing public health issue in Mexico; however, its etiology remains largely unknown. Vitamin A deficiency (VAD) and vitamin D deficiency (VDD) have been implicated in the development of anemia, though by different mechanisms. The aim of this study is to analyze the etiology of anemia and anemia-related factors in older Mexican adults. This is a cross-sectional study of 803 older adults from the southern region of Mexico in 2015. The anemia etiologies analyzed were chronic kidney disease (CKD), nutritional deficiencies (ND), anemia of inflammation (AI), anemia of multiple causes (AMC) and unexplained anemia (UEA). VAD was considered to be s-retinol ≤ 20 μg/dL, and VDD if 25(OH)D < 50 nmol/L. IL-6 and hepcidin were also measured. Multinomial regression models were generated and adjusted for confounders. Anemia was present in 35.7% of OA, independent of sex. UEA, CKD, AI and ND were confirmed in 45%, 29.3%, 14.6% and 7% of older adults with anemia, respectively. Hepcidin and log IL-6 were associated with AI (p < 0.05) and CKD (p < 0.001). VAD was associated with AI (p < 0.001), and VDD with ND and AMC (p < 0.05). Log-IL6 was associated with UEA (p < 0.001). In conclusion, anemia in older adults has an inflammatory component. VAD was associated to AI and VDD with ND and AMC.
Midwifery Qualification in Selected Countries: A Rapid Review
Authors: Md. Sharif Shakirah , Yap Wuan Shuen , Fun Weng Hong , Yoon Ee Ling , Abd Razak Nur Fadzilah , Sararaks Sondi , Lee Shaun Wen Huey
Published: October 2021

Background: While the global maternal mortality ratio (MMR) shows a decreasing trend, there is room for improvement. Midwifery education has been under scrutiny to ensure that graduates acquire knowledge and skills relevant to the local context. Objective: To review the basic professional midwifery qualification and pre-practice requirements in countries with lower MMR compared with Malaysia. Methods: A rapid review of country-specific Ministry of Health and Midwifery Association websites and Advanced Google using standardised key words. English-language documents reporting the qualifications of midwives or other requirements to practise midwifery from countries with a lower MMR than Malaysia were included. Results: Sixty-three documents from 35 countries were included. The minimum qualification required to become a midwife was a bachelor’s degree. Most countries require registration or licensing to practise, and 35.5% have implemented preregistration national midwifery examinations. In addition, 13 countries require midwives to have nursing backgrounds. Conclusion: In countries achieving better maternal outcomes than Malaysia, midwifes often have a degree or higher qualification. As such, there is a need to reinvestigate and revise the midwifery qualification requirements in Malaysia.