bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison between Different Bulk-Fill and Incremental Composite Materials Used for Class II Restorations in Primary and Permanent Teeth: In Vitro Assessments
Authors: Ibrahim Maria Salem , AlKhalefah Ahmed Saleh , Alsaghirat Abdullah Ali , Alburayh Read Ahmed , Alabdullah Nezar Ahmed
Published: October 2023

Introduction: Several advantages, including improved aesthetics and conservative cavity preparation, made resin-based composite (RBC) a popular restorative material. However, several limitations come with RBC restorations such as the necessity for proper isolation of the tooth and an incremental layering for the material due to the limitations of the depth of cure. Despite these advantages and limitations, the usage of these restorative materials is increasingly being expanded due to the advancement made since their introduction. To overcome some of the limitations, several types of RBC restorations were developed. Materials and Methods: Four different RBC materials used for class II restorations in primary and permanent teeth were compared: Z350 XT Filtek™ Universal Restorative (ZXT), Filtek™ Bulk Fill Flowable Restorative (FBF), Beautifil-Bulk Flowable (BBF) and Tetric™ N-Flow (TNF). Flexure strength, elastic modulus, surface roughness, microhardness and microleakage were assessed using standard methods or previously published protocols. The data and differences between the groups were analyzed using One-way analysis of variance (ANOVA), Tukey’s multiple comparisons, Kruskal–Wallis and Wilcoxon rank-sum (Mann–Whitney) tests. Results: The study found that BBF (86.24 ± 7.41 MPa) and ZXT (64.45 ± 11.52 MPa) had higher flexural strength than FBF (50.89 ± 8.44 MPa) and TNF (50.67 ± 9.40 MPa), while both exhibited the highest values of surface roughness. Elastic modulus was the highest with BBF, which was not statistically significant from FBF or ZXT (p > 0.05). ZXT (109.7 ± 7.83 VH) exhibited the highest value of microhardness, which was statistically significant from the other three materials (p < 0.0001). Microleakage was assessed after thermocycling for 20,000 cycles to simulate two years in the mouth. FBF (70%) exhibited the most resistance to microleakage. Conclusions: Different types of RBC restorations exhibit different characteristics. The clinician needs to choose the most appropriate restorative material based on different clinical scenarios.
Study on the Extraction Method for Track-Side Acoustic Features Based on Cyclic Stationary Analysis
Authors: Zhao Xing , Lu Yiming , Chang Baoxian , Chen Liqun
Published: October 2023

Because of its non-contact measurement characteristics, trackside acoustic technology is now utilized for train bearing fault diagnosis. However, the collected acoustic signal produces Doppler distortions that can impact the accuracy of bearing fault diagnosis. Additionally, when a fault occurs in the train bearing, it is analyzed using cyclostationary methods. In this study, we combine bearing fault characteristics with Doppler distortion correction and cyclostationary analysis methods. The trackside acoustic test platform is employed to collect and test the fault signals from bearings. These signals are processed and analyzed using Doppler distortion correction algorithms and cyclostationary techniques. A comparison between time domain maps and power spectrum maps before and after correction reveals an increase in SNR (signal to noise ratio) and a more concentrated energy distribution within the fault signals—at least a 50% improvement is observed. To further validate our method’s effectiveness, we select existing TADS equipment from a depot to collect bearing signals for analysis and processing using our proposed bearing fault diagnosis method. Comparison of time domain maps and power spectrum maps before and after correction shows clearer overall images and amplitude increase of nearly 125%. Therefore, we have successfully developed a stepwise method for bearing fault diagnosis based on cyclostationary Doppler distortion correction.
First-Order Conditions for Set-Constrained Optimization
Authors: Rovnyak Steven M. , Chong Edwin K. P. , Rovnyak James
Published: October 2023

A well-known first-order necessary condition for a point to be a local minimizer of a given function is the non-negativity of the dot product of the gradient and a vector in a feasible direction. This paper proposes a series of alternative first-order necessary conditions and corresponding first-order sufficient conditions that seem not to appear in standard texts. The conditions assume a nonzero gradient. The methods use extensions of the notions of gradient, differentiability, and twice differentiability. Examples, including one involving the Karush–Kuhn–Tucker (KKT) theorem, illustrate the scope of the conditions.
Modal Analysis of Ultrasonic Spot Welding for Lightweight Metals Joining
Authors: Huang Hui , Chen Jian , Feng Zhili , Sun Xin
Published: October 2023

Ultrasonic spot welding (USW) represents one of the unique solid-state joining methods for lightweight materials such as magnesium alloy and aluminum alloy. However, the sonotrode vibration may have a detrimental impact on the sheet material and the existing welds, depending on the component geometry and vibration frequency. In this study, a modal analysis tool based on steady-state dynamics was developed for ultrasonic spot welding which features a cyclic load applied to the sheets during the joining process. Through predicting relative motion and shear stress at the faying surfaces, coupon geometry and weld spacing are identified as two major factors that affect the welding reliability and joint quality in USW. The model was validated via welding experiments on aluminum alloy and magnesium alloy and relevant characterization of temperature distribution, joint strength as well as fracture location.
Dynamic Tensile Behavior of Laser-Directed Energy Deposition and Additive Friction Stir-Deposited AerMet 100
Authors: McClelland Zackery , Petersen Haley , Dunsford Kyle
Published: October 2023

Quasi-static and high-rate tensile experiments were used to examine the strain rate sensitivity of laser-directed energy deposition (L-DED)- and additive friction stir deposition (AFSD)-formed AerMet 100 ultrahigh-strength steel-additive manufactured builds. Electron backscattered diffraction (EBSD) revealed similar as-deposited grain sizes between the two AM processes at approximately 24 μm and 17 μm for the L-DED and AFSD samples, respectively. The strain hardening rate, θ, revealed little change in the overall hardening observed in the L-DED and AFSD materials, with a consistent hardening in the quasi-static samples and three identifiable regions in that of the high-rate tested materials. The L-DED deposited materials displayed average ultimate tensile strength values of 1835 and 2902 MPa for the 0.001 s−1 and 2500 s−1 strain rates, respectively and the AFSD deposited materials displayed ultimate tensile strength values of 1928 and 3080 MPa for the 0.001 s−1 and 2500 s−1 strain rates, respectively. Overall, the strength for both processes displayed a positive strain rate sensitivity, with increases in strength of ~1000 MPa for both processes. Fractography revealed significant solidification voids in the laser DED material and poor layer adhesion in the AFSD material.
Fracture Toughness of Ti6Al4V/Cp-Ti Multi-Material Produced via Selective Laser Melting
Authors: Repnin Arseniy , Borisov Evgenii , Emelianov Anton , Popovich Anatoliy
Published: October 2023

Multi-materials can locally enhance the properties of products to improve their performance. In some cases, it might be necessary to improve the fracture toughness properties locally. This work is devoted to investigating the fracture toughness of multi-material Ti6Al4V/Cp-Ti specimens produced via laser powder bed fusion (L-PBF). The powder feeding and distributing system of the L-PBF machine was modified for programmable dual-powder feed capability. The multi-material Ti6Al4V/Cp-Ti samples analyzed in this work are layered materials, where the Ti6Al4V alloy serves as the base material and Cp-Ti is present as separate layers. Samples of this type rely on the principle of crack inhibition, where fracture energy is dissipated in the more ductile Cp-Ti layers. Two variants of alternating ductile layers were studied. The microstructure of the materials and interfacial zone were analyzed using an optical microscope. Chemical composition was examined with a scanning electron microscope. The size of the interfacial zone in the multi-material averaged between 250 and 300 μm. A comparison of the tensile tests results with the literature data (of relatively pure Ti6Al4V alloy) reveals that there is a minor reduction in ultimate tensile strength and elongation. The obtained results confirm the possibility of locally increasing fracture toughness through the creation of a multi-material structure using L-PBF.
In Vitro Evaluation of Intestinal Transport and High-Density Fermentation of Lactobacillus acidophilus
Authors: Su Xin , Menghe Bilige , Zhang Heping , Liu Wenjun
Published: October 2023

Lactobacillus acidophilus strains have limiting factors such as low cell density and complex nutritional requirements in industrial production, which greatly restricts their industrial application. In this study, fermentation conditions for L. acidophilus were optimized and transcriptomic analysis used to understand growth mechanisms under high-density fermentation conditions. We found that L. acidophilus IMAU81186 has strong tolerance to gastrointestinal juice. In addition, its optimal culture conditions were 3% inoculum (v/v); culture temperature 37 °C; initial pH 6.5; and medium composition of 30.18 g/L glucose, 37.35 g/L soybean peptone, 18.68 g/L fish peptone, 2.46 g/L sodium citrate, 6.125 g/L sodium acetate, 2.46 g/L K2HPO4, 0.4 g/L MgSO4·7H2O,0.04 g/L MnSO4·5H2O, 0.01 g/L serine, and 0.3 g/L uracil. After optimization, viable counts of IMAU81186 increased by 7.03 times. Differentially expressed genes in IMAU81186 were analyzed at different growth stages using transcriptomics. We found that a single carbon source had limitations in improving the biomass of the strain, and terP and bfrA were significantly down-regulated in the logarithmic growth period, which may be due to the lack of extracellular sucrose. After optimizing the carbon source, we found that adding 12 g/L sucrose to the culture medium significantly increased cell density.
Chiral Metasurface Multifocal Lens in the Terahertz Band Based on Deep Learning
Authors: Wang Jingjing , Chen Sixue , Qiu Yihang , Chen Xiaoying , Shen Jian , Li Chaoyang
Published: October 2023

Chiral metasurfaces have garnered significant interest as an emerging field of metamaterials, primarily due to their exceptional capability to manipulate phase distributions at interfaces. However, the on-demand design of chiral metasurface structures remains a challenging task. To address this challenge, this paper introduces a deep learning-based network model for rapid calculation of chiral metasurface structure parameters. The network achieves a mean absolute error (MAE) of 0.025 and enables the design of chiral metasurface structures with a circular dichroism (CD) of 0.41 at a frequency of 1.169 THz. By changing the phase of the chiral metasurface, it is possible to produce not only a monofocal lens but also a multifocal lens. Well-designed chiral metasurface lenses allow us to control the number and position of focal points of the light field. This chiral metasurface, designed using deep learning, demonstrates great multifocal focus characteristics and holds great potential for a wide range of applications in sensing and holography.
Polarization Engineered p-Type Electron Blocking Layer Free AlGaN Based UV-LED Using Quantum Barriers with Heart-Shaped Graded Al Composition for Enhanced Luminescence
Authors: Das Samadrita , Lenka Trupti Ranjan , Talukdar Fazal Ahmed , Nguyen Hieu Pham Trung , Crupi Giovanni
Published: October 2023

In this paper, in order to address the problem of electron leakage in AlGaN ultra-violet light-emitting diodes, we have proposed an electron-blocking free layer AlGaN ultra-violet (UV) light-emitting diode (LED) using polarization-engineered heart-shaped AlGaN quantum barriers (QB) instead of conventional barriers. This novel structure has decreased the downward band bending at the interconnection between the consecutive quantum barriers and also flattened the electrostatic field. The parameters used during simulation are extracted from the referred experimental data of conventional UV LED. Using the Silvaco Atlas TCAD tool; version 8.18.1.R, we have compared and optimized the optical as well as electrical characteristics of three varying LED structures. Enhancements in electroluminescence at 275 nm (52.7%), optical output power (50.4%), and efficiency (61.3%) are recorded for an EBL-free AlGaN UV LED with heart-shaped Al composition in the barriers. These improvements are attributed to the minimized non-radiative recombination on the surfaces, due to the progressively increasing effective conduction band barrier height, which subsequently enhances the carrier confinement. Hence, the proposed EBL-free AlGaN LED is the potential solution to enhance optical power and produce highly efficient UV emitters.
Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of Hypsizygus marmoreus
Authors: Angelini Paola , Flores Giancarlo Angeles , Cusumano Gaia , Venanzoni Roberto , Pellegrino Roberto Maria , Zengin Gokhan , Di Simone Simonetta Cristina , Menghini Luigi , Ferrante Claudio
Published: October 2023

The beech mushroom (Hypsizygus marmoreus) is a highly nutritious, edible medicinal mushroom native to East Asia. The present research investigated the impact of different substrates on the metabolite compositions of H. marmoreus mycelia cultivated in vitro. The substrates tested included malt extract agar, malt extract agar enriched with barley malt, and malt extract agar enriched with grape pomace. The study also assessed antimicrobial and antiradical activities of the extracts against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa), yeasts (Candida albicans, C. tropicalis, and C. parapsilosis), and dermatophytes (Trichophyton mentagrophytes, T. tonsurans, T. rubrum, Arthroderma quadrifidum, A. gypseum, A. curreyi, and A. insingulare). The results revealed that the H. marmoreus mycelia extracts demonstrated antibacterial and antifungal activities against the tested microorganisms. Extracts obtained from the cultivation in substrates enriched with either barley malt or grape pomace exhibited the highest antibacterial activity among all the tested bacterial strains except for P. aeruginosa. The same extracts showed the highest inhibitory effect against C. albicans and C. parapsilosis. Noteworthy, the extract from the mushroom cultivated in the substrate enriched with grape pomace also exhibited remarkable efficacy against T. mentagrophytes and T. tonsurans. Terpenoid and carbapenem compounds could be related to the antimicrobial properties of the extracts from mushrooms cultivated in substrates enriched with grape pomace. In comparison, the higher antiradical properties could be related to the content of indole compounds. In conclusion, growth substrate selection affects the nutritional and medicinal properties of H. marmoreus, making it a valuable contribution to the understanding of the cultivation of this mushroom.
CPGminer: An Interactive Dashboard to Explore the Genomic Features and Taxonomy of Complete Prokaryotic Genomes
Authors: Kim Jaehyun , Yoon Sunghyun , Kondakala Sandeep , Foley Steven , Hart Mark , Baek Dong-Heon , Wang Wenjun , Kim Sung-Kwan , Sutherland John , Kim Seong-Jae , Kweon Ohgew
Published: October 2023

Prokaryotes, the earliest forms of life on Earth, play crucial roles in global biogeochemical processes in virtually all ecosystems. The ever-increasing amount of prokaryotic genome sequencing data provides a wealth of information to examine fundamental and applied questions through systematic genome comparison. Genomic features, such as genome size and GC content, and taxonomy-centric genomic features of complete prokaryotic genomes (CPGs) are crucial for various fields of microbial research and education, yet they are often overlooked. Additionally, creating systematically curated datasets that align with research concerns is an essential yet challenging task for wet-lab researchers. In this study, we introduce CPGminer, a user-friendly tool that allows researchers to quickly and easily examine the genomic features and taxonomy of CPGs and curate genome datasets. We also provide several examples to demonstrate its practical utility in addressing descriptive questions.
Study on the Reverse Flotation Separation of Smithsonite from Dolomite Using the Saponified 2-(4,4-Dimethylpentan-2-yl)-5,7,7-trimethyloctanoic Acid as a Collector
Authors: Zhang Xiaoliang , Zhu Yangge , Zhao Zhiqiang , Wang Peilong
Published: October 2023

Dolomite, a prominent calcium-bearing gangue mineral found in carbonate-type zinc oxide ores, poses a significant challenge for effective flotation separation alongside smithsonite due to their highly similar surface properties. The present study explores the potential of 2-(4,4-dimethylpentan-2-yl)-5,7,7-trimethyloctanoic acid (2-DMPT) as a collector for the reverse flotation of smithsonite from dolomite. Micro-flotation experiments indicated that saponified 2-DMPT exhibited superior collecting ability and selectivity for dolomite over smithsonite under highly alkaline conditions. Specifically, the flotation recovery of dolomite reached 62%, whereas only 6% of smithsonite was recovered in the flotation foam products. Zeta potential and attenuated total reflectance–Fourier transform infrared (ATR-FTIR) analysis revealed that changes in pH values had minimal influence on the collector’s adsorption onto dolomite, while significantly hindering its adsorption on the smithsonite surface. Furthermore, X-ray photoelectron spectroscopy (XPS) analysis showed that 2-DMPT could form hydrophobic complexes with the active Ca2+ sites on the dolomite surface at pH 11.5. However, the interaction between smithsonite and carboxylic groups of 2-DMPT under the same conditions was relatively weaker, facilitating their reverse flotation separation. As a result, 2-DMPT shows promise as a potential collector for the reverse flotation process, effectively removing dolomite from smithsonite and reducing acid consumption in subsequent acid-leaching processes.
The Hybrid Nano-Biointerface between Proteins/Peptides and Two-Dimensional Nanomaterials
Authors: Forte Giuseppe , La Mendola Diego , Satriano Cristina
Published: October 2023

In typical protein–nanoparticle surface interactions, the biomolecule surface binding and consequent conformational changes are intermingled with each other and are pivotal to the multiple functional properties of the resulting hybrid bioengineered nanomaterial. In this review, we focus on the peculiar properties of the layer formed when biomolecules, especially proteins and peptides, face two-dimensional (2D) nanomaterials, to provide an overview of the state-of-the-art knowledge and the current challenges concerning the biomolecule coronas and, in general, the 2D nano-biointerface established when peptides and proteins interact with the nanosheet surface. Specifically, this review includes both experimental and simulation studies, including some recent machine learning results of a wide range of nanomaterial and peptide/protein systems.
Advance Progress in Assembly Mechanisms of Carrier-Free Nanodrugs for Cancer Treatment
Authors: Zhang Xiaoyu , Hu Shuyang , Huang Lifei , Chen Xiyue , Wang Xin , Fu Ya-nan , Sun Hui , Li Guofeng , Wang Xing
Published: October 2023

Nanocarriers have been widely studied and applied in the field of cancer treatment. However, conventional nanocarriers still suffer from complicated preparation processes, low drug loading, and potential toxicity of carriers themselves. To tackle the hindrance, carrier-free nanodrugs with biological activity have received increasing attention in cancer therapy. Extensive efforts have been made to exploit new self-assembly methods and mechanisms to expand the scope of carrier-free nanodrugs with enhanced therapeutic performance. In this review, we summarize the advanced progress and applications of carrier-free nanodrugs based on different types of assembly mechanisms and strategies, which involved noncovalent interactions, a combination of covalent bonds and noncovalent interactions, and metal ions-coordinated self-assembly. These carrier-free nanodrugs are introduced in detail according to their assembly and antitumor applications. Finally, the prospects and existing challenges of carrier-free nanodrugs in future development and clinical application are discussed. We hope that this comprehensive review will provide new insights into the rational design of more effective carrier-free nanodrug systems and advancing clinical cancer and other diseases (e.g., bacterial infections) infection treatment.
Enhancing Bioactivity and Conjugation in Green Coffee Bean (Coffea arabica) Extract through Cold Plasma Treatment: Insights into Antioxidant Activity and Phenolic–Protein Conjugates
Authors: Kungsuwan Kuntapas , Sawangrat Choncharoen , Ounjaijean Sakaewan , Chaipoot Supakit , Phongphisutthinant Rewat , Wiriyacharee Pairote
Published: October 2023

Cold plasma technology is gaining attention as a promising approach to enhancing the bioactivity of plant extracts. However, its impact on green coffee bean extracts (GCBEs) still needs to be explored. In this study, an innovative underwater plasma jet system was employed to investigate the effects of cold plasma on Coffea arabica GCBEs, focusing on the conjugation reflected by the change in composition and bioactivity. The DPPH radical scavenging antioxidant activity exhibited a gradual increase with plasma treatment up to 35 min, followed by a decline. Remarkably, at 35 min, the plasma treatment resulted in a significant 66% increase in the DPPH radical scavenging activity of the GCBE. The total phenolic compound content also displayed a similar increasing trend to the DPPH radical scavenging activity. However, the phenolic profile analysis indicated a significant decrease in chlorogenic acids and caffeine. Furthermore, the chemical composition analysis revealed a decrease in free amino acids, while sucrose remained unchanged. Additionally, the SDS-PAGE results suggested a slight increase in protein size. The observed enhancement in antioxidant activity, despite the reduction in the two major antioxidants in the GCBE, along with the increase in protein size, might suggest the occurrence of conjugation processes induced by plasma, particularly involving proteins and phenolic compounds. Notably, the plasma treatment exhibited no adverse effects on the extract’s safety, as confirmed by the MTT assay. These findings indicate that cold plasma treatment holds significant promise in improving the functional properties of GCBE while ensuring its safety. Incorporating cold plasma technology into the processing of natural extracts may offer exciting opportunities for developing novel and potent antioxidant-rich products.
Montmorillonite Catalyzed Synthesis of Novel Steroid Dimers
Authors: Tomkiel Aneta M. , Majewski Adam D. , Siergiejczyk Leszek , Morzycki Jacek W.
Published: October 2023

The reactions of sterols (androst-5-en-3β-ol-17-one, diosgenin, and cholesterol) and their tosylates with hydroquinone aimed at the synthesis of O,O-1,4-phenylene-linked steroid dimers were studied. The reaction course strongly depended on the conditions used. The study has shown that the major reaction products are the elimination products and unusual steroid dimers resulting from the nucleophilic attack of the hydroquinone C2 carbon atom on the steroid C3 position, followed by an intramolecular addition to the C5–C6 double bond. A different reaction course was observed when montmorillonite K10 was used as a catalyst. The reaction of androst-5-en-3β-ol-17-one under the promotion of this catalyst afforded the O,O-1,4-phenylene-linked steroid dimer in addition to the disteroidal ether. The formation of the latter compound was suppressed by using 3-tosylate as a substrate instead of the free sterol. The reactions of androst-5-en-3β-ol-17-one tosylate and cholesteryl tosylate with hydroquinone catalyzed by montmorillonite K10 carried out under optimized conditions afforded the desired dimers in 31% and 67% yield, respectively.
Metal Electrodes for Filtering the Localized Fundamental Mode of a Ridge Optical Waveguide on a Thin Lithium Niobate Nanofilm
Authors: Parfenov Mikhail , Agruzov Petr , Tronev Aleksandr , Ilichev Igor , Usikova Anna , Zadiranov Yurii , Shamrai Aleksandr
Published: October 2023

An approach for filtering the fundamental mode in an integrated optical modulator with multimode waveguides based on etched thin lithium niobate nanofilms is presented. It is shown that metal electrodes can be used as a modal filter to suppress high-order modes in wide multimode ridge waveguides and, consequently, to provide their quasi-single-mode regime of operation. The influence of the gap between the electrodes and its displacement relative to the waveguide symmetry axis is analyzed for various configurations of waveguides. The conditions for quasi-single-mode light propagation with suppression of high-order modes of more than 90 dB/cm are found. The influence of fabrication errors on the efficiency of modal filtering is discussed. Efficient electro-optical modulation with an equivalent voltage-length product of 4 V∙cm has been experimentally demonstrated on integrated optical phase modulator samples fabricated using conventional contact photolithography. The proposed topological solution can be further used for the fast and cheap fabrication of TFLN modulators by conventional contact photolithography. The proposed modal filtering can also be used in other waveguide topologies and in more complex waveguide devices.
Ketogenic Diet and Breast Cancer: Recent Findings and Therapeutic Approaches
Authors: Urzì Alfio Giuseppe , Tropea Emanuela , Gattuso Giuseppe , Spoto Graziana , Marsala Gabriella , Calina Daniela , Libra Massimo , Falzone Luca
Published: October 2023

Breast cancer (BC), a complex disease with several influencing factors, is significantly impacted by dietary habits. The ketogenic diet (KD), characterized by high fat and low carbohydrate intake, has gained attention as a potential therapeutic approach, but its effects on BC remain unclear. This review seeks to summarize the current knowledge on the principles of the KD, its metabolic influence on BC cells, and the findings of recent clinical trials, in order to elucidate the potential therapeutic role of the KD in BC management. For these purposes, a comprehensive literature review was conducted selecting preclinical and clinical studies that investigate the relationship between the KD and BC. The selection criteria prioritized studies exploring the KD’s metabolic effects on BC cells and current clinical trials involving the KD in BC management. The reviewed studies provide a diverse range of findings, with some suggesting potential benefits of the KD in inhibiting tumor growth and improving treatment response. However, robust clinical trials providing clear evidence of the KD’s efficacy as a standalone therapeutic approach in BC are still lacking. There are also significant concerns regarding the safety and long-term effects of sustained ketosis in cancer patients. The therapeutic potential of the KD in BC remains an area of active research and debate. While preliminary findings are promising, definitive conclusions are hindered by inconsistent results and limited human trial data. Future research, specifically well-structured, large-scale clinical trials, is necessary to provide a comprehensive understanding of the role of the KD in BC treatment. Until then, caution should be exercised in its application, and patients should continue prioritizing evidence-based, standard-of-care treatments.
Salt Intake Estimation from Urine Samples in South Asian Population: Scoping Review
Authors: Afroza Ummay , Abrar Ahmad Khairul , Nowar Abira , Akhtar Jubaida , Mamun Mohammad Abdullah Al , Sobhan Sheikh Mohammad Mahbubus , Cobb Laura , Ide Nicole , Choudhury Sohel Reza
Published: October 2023

The World Health Organization recommended reducing one’s salt intake below 5 g/day to prevent disability and death from cardiovascular and other chronic diseases. This review aimed to identify salt estimation at the population level in South Asian countries, namely Afghanistan, Bangladesh, Bhutan, India, Nepal, Pakistan, and Sri Lanka. We searched electronic databases and government websites for the literature and reports published between January 2011 and October 2021 and also consulted key informants for unpublished reports. We included studies that assessed salt intake from urinary sodium excretion, either spot urine or a 24 h urine sample, on a minimum of 100 samples in South Asian countries. We included 12 studies meeting the criteria after screening 2043 studies, out of which five followed nationally representative methods. This review revealed that salt intake in South Asian countries ranges from 6.7–13.3 g/day. The reported lowest level of salt intake was in Bangladesh and India, and the highest one was in Nepal. The estimated salt intake reported in the nationally representative studies were ranging from 8 g/day (in India) to 12.1 g/day (in Afghanistan). Salt consumption in men (8.9–12.5 g/day) was reported higher than in women (7.1–12.5 g/day). Despite the global target of population salt intake reduction, people in South Asian countries consume a much higher amount of salt than the WHO-recommended level.
Reduced Relapse-Free Survival in Colorectal Cancer Patients with Elevated Soluble CD40 Ligand Levels Improved by Vitamin D Supplementation
Authors: Fujimoto Hiroshi , Fukuzato Soichiro , Kanno Kazuki , Akutsu Taisuke , Ohdaira Hironori , Suzuki Yutaka , Urashima Mitsuyoshi
Published: October 2023

Although elevated serum levels of soluble CD40 ligand (sCD40L) were reported in patients with cancer, the importance of high sCD40L levels in clinical oncology remains unknown. We conducted a post hoc analysis of the AMATERASU randomized clinical trial of vitamin D3 supplementation (2000 IU/day) in patients with digestive tract cancer to assess its significance. Serum sCD40L levels were measured by ELISA in 294 residual samples, and were divided into tertiles. In patients with colorectal cancer (CRC), 5-year relapse-free survival (RFS) rates in the middle and highest tertiles were 61.6% and 61.2%, respectively, which was significantly lower than 83.8% in the lowest tertile. A Cox proportional hazard analysis showed that the lowest tertile had a significantly lower risk of relapse or death than the highest tertile even with multivariate adjustment (hazard ratio (HR), 0.30; 95% confidence interval (CI), 0.11–0.80; p = 0.016). In the subgroup of CRC patients with the highest tertile of sCD40L, the 5-year RFS rate in the vitamin D group was 77.9%, which was significantly higher than 33.2% in the placebo group (HR, 0.30; 95% CI, 0.11–0.81; p = 0.018 [Pinteraction = 0.04]). In conclusion, elevated sCD40L might be a biomarker of poor prognosis in patients with CRC, but vitamin D supplementation might improve RFS in patients with high sCD40L.
Novel Baicalein-Derived Inhibitors of Plasmodium falciparum
Authors: Gudla Chandra Sekhar , Selvam Vignesh , Selvaraj Siva Shanmugam , Tripathi Renu , Joshi Prince , Shaham Salique Hassan , Singh Mayas , Shandil Radha Krishan , Habib Saman , Narayanan Shridhar
Published: October 2023

Malaria, a life-threatening mosquito-borne disease caused by Plasmodium parasites, continues to pose a significant global health burden. Despite notable progress in combating the disease in recent years, malaria remains prevalent in many regions, particularly in Southeast Asia and most of sub-Saharan Africa, where it claims hundreds of thousands of lives annually. Flavonoids, such as the baicalein class of compounds, are known to have antimalarial properties. In this study, we rationally designed and synthesized a series of baicalein derivatives and identified a lead compound, FNDR-10132, that displayed potent in vitro antimalarial activity against Plasmodium falciparum (P. falciparum), both chloroquine-sensitive (60 nM) and chloroquine-resistant (177 nM) parasites. FNDR-10132 was evaluated for its antimalarial activity in vivo against the chloroquine-resistant strain Plasmodium yoelii N67 in Swiss mice. The oral administration of 100 mg/kg of FNDR-10132 showed 44% parasite suppression on day 4, with a mean survival time of 13.5 ± 2.3 days vs. 8.4 ± 2.3 days of control. Also, FNDR-10132 displayed equivalent activity against the resistant strains of P. falciparum in the 200–300 nM range. This study offers a novel series of antimalarial compounds that could be developed into potent drugs against chloroquine-resistant malarial parasites through further chemistry and DMPK optimization.
Combined Lycium barbarum Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells
Authors: Lian Yu Zhi , Liu Yu-Chuan , Chang Chun-Chao , Nochi Tomonori , Chao Jane C.-J.
Published: October 2023

The effects of Lycium barbarum polysaccharides (LBP) and plasmon-activated water (PAW) against IFN-γ/TNF-α induced inflammation in human colon Caco-2 cells were investigated. Cells were divided into the control, induction, LBP treatment (100–500 μg/mL), and combination groups with PAW. Inflammation was induced 24 h with 10 ng/mL IFN-γ when cell confluency reached >90%, and various doses of LBP with or without PAW were treated for 3 h, and subsequently 50 ng/mL TNF-α was added for another 24 h to provoke inflammation. Combination of LBP with PAW significantly decreased the secretion of IL-6 and IL-8. Cyclooxygenase-2 and inducible NO synthase expression was attenuated in all LBP-treated groups with or without PAW. NLRP3 inflammasome and related protein PYCARD expression were inhibited by LBP at the highest dose (500 μg/mL). All doses of LBP alone significantly decreased p-ERK expression, but combination with PAW increased p-ERK expression compared to those without PAW. Additionally, 250 and 500 μg/mL of LBP with or without PAW inhibited procaspase-3/caspase-3 expression. Therefore, LBP possesses anti-inflammation and anti-apoptosis by inhibiting the secretion of inflammatory cytokines and the expression of NLRP3 inflammasome-related protein. The combination with PAW exerts additive or synergistic effect on anti-inflammation.
Isolation and Characterization of an Anti-Osteoporotic Compound from Melia toosendan Fructus
Authors: Kim Seong Cheol , Gu Dong Ryun , Yang Hyun , Lee Sung-Ju , Ryuk Jin Ah , Ha Hyunil
Published: October 2023

Melia toosendan fructus, traditionally employed in traditional Chinese and Korean herbal medicine, exhibits diverse biological properties encompassing anti-tumor, anti-inflammatory, and anti-viral effects. However, its influence on bone metabolism remains largely unexplored. In this study, we investigated the impact of an ethanolic extract of Melia toosendan fructus (MTE) on osteoclast differentiation and characterized its principal active constituent in osteoclast differentiation and function, as well as its effects on bone protection. Our findings demonstrate that MTE effectively inhibits the differentiation of osteoclast precursors induced by receptor activator of nuclear factor κB ligand (RANKL). Utilizing a bioassay-guided fractionation approach coupled with UHPLC-MS/MS analysis, we isolated and identified the triterpenoid compound toosendanin (TSN) as the active constituent responsible for MTE’s anti-osteoclastogenic activity. TSN treatment downregulated the expression of nuclear factor of activated T cells c1, a pivotal osteoclastogenic transcription factor, along with molecules implicated in osteoclast-mediated bone resorption, including tumor necrosis factor receptor-associated factor 6, carbonic anhydrase II, integrin beta-3, and cathepsin K. Furthermore, treatment of mature osteoclasts with TSN impaired actin ring formation, acidification, and resorptive function. Consistent with our in vitro findings, TSN administration mitigated trabecular bone loss and reduced serum levels of the bone resorption marker, C-terminal cross-linked telopeptides of type I collagen, in a mouse bone loss model induced by intraperitoneal injections of RANKL. These results suggest that TSN, as the principal active constituent of MTE with inhibitory effects on osteoclastogenesis, exhibits bone-protective properties by suppressing both osteoclast differentiation and function. These findings imply the potential utility of TSN in the treatment of diseases characterized by excessive bone resorption.
Immunoengineering via Chimeric Antigen Receptor-T Cell Therapy: Reprogramming Nanodrug Delivery
Authors: Katopodi Theodora , Petanidis Savvas , Anestakis Doxakis , Charalampidis Charalampos , Chatziprodromidou Ioanna , Floros George , Eskitzis Panagiotis , Zarogoulidis Paul , Koulouris Charilaos , Sevva Christina , Papadopoulos Konstantinos , Dagher Marios , Varsamis Nikolaos , Theodorou Vasiliki , Mystakidou Chrysi Maria , Katsios Nikolaos Iason , Farmakis Konstantinos , Kosmidis Christoforos
Published: October 2023

Following its therapeutic effect in hematological metastasis, chimeric antigen receptor (CAR) T cell therapy has gained a great deal of attention during the last years. However, the effectiveness of this treatment has been hampered by a number of challenges, including significant toxicities, difficult access to tumor locations, inadequate therapeutic persistence, and manufacturing problems. Developing novel techniques to produce effective CARs, administer them, and monitor their anti-tumor activity in CAR-T cell treatment is undoubtedly necessary. Exploiting the advantages of nanotechnology may possibly be a useful strategy to increase the efficacy of CAR-T cell treatment. This study outlines the current drawbacks of CAR-T immunotherapy and identifies promising developments and significant benefits of using nanotechnology in order to introduce CAR transgene motifs into primary T cells, promote T cell expansion, enhance T cell trafficking, promote intrinsic T cell activity and rewire the immunosuppressive cellular and vascular microenvironments. Therefore, the development of powerful CART cells can be made possible with genetic and functional alterations supported by nanotechnology. In this review, we discuss the innovative and possible uses of nanotechnology for clinical translation, including the delivery, engineering, execution, and modulation of immune functions to enhance and optimize the anti-tumor efficacy of CAR-T cell treatment.
Interplay of Extracellular Vesicles and TLR4 Signaling in Hepatocellular Carcinoma Pathophysiology and Therapeutics
Authors: Papadakos Stavros P. , Arvanitakis Konstantinos , Stergiou Ioanna E. , Vallilas Christos , Sougioultzis Stavros , Germanidis Georgios , Theocharis Stamatios
Published: October 2023

Hepatocellular carcinoma (HCC) stands as a significant contributor to global cancer-related mortality. Chronic inflammation, often arising from diverse sources such as viral hepatitis, alcohol misuse, nonalcoholic fatty liver disease (NAFLD), and nonalcoholic steatohepatitis (NASH), profoundly influences HCC development. Within this context, the interplay of extracellular vesicles (EVs) gains prominence. EVs, encompassing exosomes and microvesicles, mediate cell-to-cell communication and cargo transfer, impacting various biological processes, including inflammation and cancer progression. Toll-like receptor 4 (TLR4), a key sentinel of the innate immune system, recognizes both pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs), thereby triggering diverse signaling cascades and pro-inflammatory cytokine release. The intricate involvement of the TLR4 signaling pathway in chronic liver disease and HCC pathogenesis is discussed in this study. Moreover, we delve into the therapeutic potential of modulating the TLR4 pathway using EVs as novel therapeutic agents for HCC. This review underscores the multifaceted role of EVs in the context of HCC and proposes innovative avenues for targeted interventions against this formidable disease.
Intravitreal DEX Implant for the Treatment of Diabetic Macular Edema: A Review of National Consensus
Authors: Spinetta Roberta , Petrillo Francesco , Reibaldi Michele , Tortori Antonia , Mazzoni Maria , Metrangolo Cristian , Gelormini Francesco , Ricardi Federico , Giordano Antonio
Published: October 2023

Diabetic macular edema (DME)’s therapeutic approach can frequently be challenging. The purpose of the review is to propose evidence-based recommendations on the employment of intravitreal dexamethasone implants (DEX) when approaching patients suffering from DME. Seven national consensuses redacted by different groups of retina specialists from Europe and Asia were examined and confronted. Each consensus was redacted utilizing a Delphi approach, in person meetings, or by reviewing the literature. DEX can be studied as a first-line strategy in individuals suffering from DME with inflammatory OCT biomarkers, in vitrectomized eyes, in patients with recent cardiovascular events, in pregnant women, in patients scheduled to undergo cataract surgery or with poor compliance. The other parameters considered were the indications to the DME treatment, when to switch to DEX, the definition of non-responder to anti-VEGFs agents and to the DEX implant, whether to combine DEX with laser photocoagulation, the association between glaucoma and DEX, and the management of DEX and the cataract. Although several years have passed since the introduction of DEX implants in the DME treatment, there is still not a unified agreement among retina specialists. This paper compares the approach in the DME treatment between countries from different continents and provides a broader and worldwide perspective of the topic.
Two-Dimensional Dynamic Beam Steering by Tamm Plasmon Polariton
Authors: Bikbaev Rashid G. , Chen Kuo-Ping , Timofeev Ivan V.
Published: October 2023

The dynamic steering of a beam reflected from a photonic structure supporting Tamm plasmon polariton is demonstrated. The phase and amplitude of the reflected wave are adjusted by modulating the refractive index of a transparent conductive oxide layer by applying a bias voltage. It is shown that the proposed design allows for two-dimensional beam steering by deflecting the light beam along the polar and azimuthal angles.
Synthetic Polyisoprene Rubber as a Mimic of Natural Rubber: Recent Advances on Synthesis, Nanocomposites, and Applications
Authors: Cruz-Morales Jorge A. , Gutiérrez-Flores Carina , Zárate-Saldaña Daniel , Burelo Manuel , García-Ortega Héctor , Gutiérrez Selena
Published: October 2023

Up to now, rubber materials have been used in a wide range of applications, from automotive parts to special-design engineering pieces, as well as in the pharmaceutical, food, electronics, and military industries, among others. Since the discovery of the vulcanization of natural rubber (NR) in 1838, the continuous demand for this material has intensified the quest for a synthetic substitute with similar properties. In this regard, synthetic polyisoprene rubber (IR) emerged as an attractive alternative. However, despite the efforts made, some properties of natural rubber have been difficult to match (i.e., superior mechanical properties) due not only to its high content of cis-1,4-polyisoprene but also because its structure is considered a naturally occurring nanocomposite. In this sense, cutting-edge research has proposed the synthesis of nanocomposites with synthetic rubber, obtaining the same properties as natural rubber. This review focuses on the synthesis, structure, and properties of natural and synthetic rubber, with a special interest in the synthesis of IR nanocomposites, giving the reader a comprehensive reference on how to achieve a mimic of NR.
Coating of Felt Fibers with Carbon Nanotubes and PEDOT with Different Counterions: Temperature and Electrical Field Effects
Authors: Carsí Marta , Sanchis María J. , Serrano-Claumarchirant José F. , Culebras Mario , Gómez Clara M.
Published: October 2023

The use of wearable devices has promoted new ways of integrating these devices, one of which is through the development of smart textiles. Smart textiles must possess the mechanical and electrical properties necessary for their functionality. This study explores the impact of polymer-felt microstructure variations on their morphology, electrical, and mechanical properties. The application of thermal treatment, along with an electric field, leads to a substantial structural reorganization of the molecular chains within pristine felt. This results in a system of nanofibrils coated with MWCNT-PEDOT, characterized by highly ordered counterions that facilitate the flow of charge carriers. Both temperature and an electric field induce reversible microstructural changes in pristine felt and irreversible changes in coated felt samples. Furthermore, electropolymerization of PEDOT significantly enhances electrical conductivity, with PEDOT:BTFMSI-coated fabric exhibiting the highest conductivity.
Composites with Re-Entrant Lattice: Effect of Filler on Auxetic Behaviour
Authors: Tashkinov Mikhail , Tarasova Anastasia , Vindokurov Ilia , Silberschmidt Vadim V.
Published: October 2023

This study is focused on the deformation behaviour of composites formed by auxetic lattice structures acting as a matrix based on the re-entrant unit-cell geometry with a soft filler, motivated by biomedical applications. Three-dimensional models of two types of the auxetic-lattice structures were manufactured using filament deposition modelling. Numerical finite-element models were developed for computational analysis of the effect of the filler with different mechanical properties on the effective Poisson’s ratio and mechanical behaviour of such composites. Tensile tests of 3D-printed auxetic samples were performed with strain measurements using digital image correlation. The use of the filler phase with various elastic moduli resulted in positive, negative, and close-to-zero effective Poisson’s ratios. Two approaches for numerical measurement of the Poisson’s ratio were used. The failure probability of the two-phase composites with auxetic structure depending on the filler stiffness was investigated by assessing statistical distributions of stresses in the finite-elements models.