bluuebunny/crossref_metadata_2025 huggingface dataset:
Stemming the Rise of Antibiotic Use for Community-Acquired Acute Respiratory Infections during COVID-19 Pandemic
Authors: Lim Shena Y. C. , Zhou Yvonne P. , Yii Daphne , Chin De Zhi , Hung Kai Chee , Lee Lai Wei , Lim Jia Le , Loo Li Wen , Koomanan Narendran , Chua Nathalie Grace , Liew Yixin , Cherng Benjamin P. Z. , Thien Siew Yee , Lee Winnie H. L. , Kwa Andrea L. H. , Chung Shimin J.
Published: June 2022

At the start of the COVID-19 pandemic, there was an increase in the use of antibiotics for the treatment of community-acquired respiratory tract infection (CA-ARI) in patients admitted for suspected or confirmed COVID-19, raising concerns for misuse. These antibiotics are not under the usual purview of the antimicrobial stewardship unit (ASU). Serum procalcitonin, a biomarker to distinguish viral from bacterial infections, can be used to guide antibiotic recommendations in suspected lower respiratory tract infection. We modified our stewardship approach, and used a procalcitonin-guided strategy to identify “high yield” interventions for audits in patients admitted with CA-ARI. With this approach, there was an increase in the proportion of patients with antibiotics discontinued within 4 days (16.5% vs. 34.9%, p < 0.001), and the overall duration of antibiotic therapy was significantly shorter [7 (6–8) vs. 6 (3–8) days, p < 0.001]. There was a significant decrease in patients with intravenous-to-oral switch of antibiotics to “complete the course” (45.3% vs. 34.4%, p < 0.05). Of the patients who had antibiotics discontinued, none were restarted on antibiotics within 48 h, and there was no-30-day readmission or 30-day mortality attributed to respiratory infection. This study illustrates the importance of the antimicrobial stewardship during the pandemic and the need for ASU to remain attuned to prescriber’s practices, and adapt accordingly to address antibiotic misuse to curb antimicrobial resistance.
Antibiotic (Mis)Use in COVID-19 Patients before and after Admission to a Tertiary Hospital in Serbia
Authors: Despotović Aleksa , Barać Aleksandra , Cucanić Teodora , Cucanić Ksenija , Stevanović Goran
Published: June 2022

Antimicrobial resistance (AMR) is a global concern, and antibiotic use has risen throughout the COVID-19 pandemic. Up to 75% of COVID-19 patients are treated with antibiotics despite little evidence for their use. A retrospective study from 6 March 2020 (the start of the pandemic in Serbia) to 31 December 2021 was conducted at the Clinic for Infectious and Tropical Diseases, University Clinical Centre of Serbia. In total, 523 patients with a microbiological diagnosis of COVID-19 were included. Patient data were analysed, including antibiotic use before and after admission. Pre-admission use of antibiotics for COVID-19 treatment was documented in more than half of patients (58.1%), of which a third (34.1%) used more than one antibiotic. Macrolides, cephalosporins, and fluoroquinolones were mainly used, most frequently among patients aged between 31–45 years (75.2%). Prior antibiotic use was associated with a longer duration of illness at admission (8.8 vs. 5.7, p < 0.001), oxygen therapy upon admission (27.6% vs. 16.0%, p = 0.002), and a lower vaccination rate (60.7% vs. 50.7%, p = 0.04). When hospitalised, 72.1% of patients received antibiotics, primarily cephalosporins (71.9%). Significant efforts are needed to reduce antibiotic use in the community and improve prescribing rates by healthcare professionals.
The Array of Antibacterial Action of Protocatechuic Acid Ethyl Ester and Erythromycin on Staphylococcal Strains
Authors: Miklasińska-Majdanik Maria , Kępa Małgorzata , Kulczak Monika , Ochwat Maciej , Wąsik Tomasz J.
Published: June 2022

The spread of antibiotic resistance among bacteria has become one of the major health problems worldwide. Methicillin-resistant staphylococcal strains are especially dangerous because they are often resistant to other antibiotics. The increasing insensitivity to macrolides, lincosamides and streptogramin B antibiotics of methicillin-resistant staphylococcal isolates has limited the use of these drugs in therapy. The combination of natural compounds and antibiotics can be considered as an alternative tool to fight multi-drug-resistant pathogen infections. The aim of the presented study was to examine the antibacterial activity of protocatechuic acid ethyl ester–erythromycin combination towards Staphylococcus aureus and Staphylococcus epidermidis strains with various resistance profiles to methicillin and macrolides, lincosamides and streptogramin B (MLSB) antibiotics. The in-vitro antibacterial potential of the above combination was investigated by minimum inhibitory concentration assays and checkerboard testing. The observed effects were strain dependent, with 8 of 12 tested staphylococcal strains showing an indifferent effect on the natural compound and erythromycin; for 2 strains, the tested combination had an additive effect, while for another 2, the effect was synergistic. Interestingly, the multi-drug-resistant strains were more sensitive to the cooperative action of the protocatechuic acid ethyl ester and the antibiotic.
Bacterial Colonization Incidence before and after Indwelling Double-J Ureteral Stents
Authors: Kaliyeva Sholpan , Simokhina Natalya , Lavrinenko Alyona , Zhussupova Gulzira , Zhunusov Serik , Semenikhina Polina , Bikbatyrova Yuliya , Yelmagambetov Berik , Myasnikova Zhanna
Published: June 2022

The upper urinary tract stenting allows to restore the ureteral patency in various situations. However, one of the main disadvantages of stenting is bacterial contamination, which can be a source of persistent infections that hardly respond to antibiotic therapy. The aim of this study was to investigate the local spectrum of bacterial pathogens and their susceptibility to antibiotics in order to optimize antibacterial therapy after upper urinary tract stenting. A prospective observational study was conducted in which 140 urine samples were examined (70 before stenting and 70 after stenting). Bacterial growth was detected in 37 patients (52.8%) before stenting and in 43 patients (61.4%) after stenting. E. coli (13 (28.8%)) and Streptococcus spp. (8 (17.6%)) strains were more commonly detected before stenting; P. aeruginosa (15 (31.2%)) and E. coli (8 (16.6%)) were usually revealed after stenting. The proportion of P. aeruginosa strains after stenting grew from 4.4% up to 31.2%. E. coli strains were resistant to ampicillin (92.3% before and 100% after stenting). Three strains of E. coli (23.1%) and six strains of P. aeruginosa (40%) were multidrug-resistant. Determination of the bacterial sensitivity to antibiotics and identification of antibiotic-resistant forms of bacteria is a factor in reducing the risk of complications and optimizing antibiotic therapy during the upper urinary tract stenting.
Ventilator-Associated Pneumonia Due to MRSA vs. MSSA: What Should Guide Empiric Therapy?
Authors: Colaneri Marta , Di Carlo Domenico , Amatu Alessandro , Marvulli Lea Nadia , Corbella Marta , Petazzoni Greta , Cambieri Patrizia , Muzzi Alba , Bandi Claudio , Di Matteo Angela , Sacchi Paolo , Mojoli Francesco , Bruno Raffaele
Published: June 2022

The guidelines on ventilator-associated pneumonia (VAP) recommend an empiric therapy against methicillin-resistant Staphylococcus aureus (MRSA) according to its prevalence rate. Considering the MRSA and MSSA VAP prevalence over the last 9 years in our tertiary care hospital, we assessed the clinical value of the MRSA nasal-swab screening in either predicting or ruling out MRSA VAP. We extracted the data of 1461 patients with positive bronchoalveolar lavage (BAL). Regarding the MRSA nasal-swab screening, 170 patients were positive for MRSA or MSSA. Overall, MRSA had a high prevalence in our ICU. Despite the COVID-19 pandemic, there was a significant downward trend in MRSA prevalence, while MSSA remained steady over time. Having VAP due to MRSA did not have any impact on LOS and mortality. Finally, the MRSA nasal-swab testing demonstrated a very high negative predictive value for MRSA VAP. Our results suggested the potential value of a patient-centered approach to improve antibiotic stewardship.
Biosynthesized Silver Nanoparticles from Eruca sativa Miller Leaf Extract Exhibits Antibacterial, Antioxidant, Anti-Quorum-Sensing, Antibiofilm, and Anti-Metastatic Activities
Authors: Awadelkareem Amir Mahgoub , Al-Shammari Eyad , Elkhalifa AbdElmoneim O. , Adnan Mohd , Siddiqui Arif Jamal , Patel Mitesh , Khan Mohammad Idreesh , Mehmood Khalid , Ashfaq Fauzia , Badraoui Riadh , Ashraf Syed Amir
Published: June 2022

Worldwide, the primary problem today is the proliferation of cancer and secondary bacterial infections caused by biofilms, as they are the principal causes of death due to the lack of effective drugs. A great deal of biological activities of silver nanoparticles (AgNPs) have made them a brilliant choice for the development of new drugs in recent years. The present study was conducted to evaluate the anticancer, antibacterial, anti-QS, and antibiofilm effects of AgNPs synthesized from Eruca sativa (E. sativa) leaf extract. The ultraviolet–visible (UV–Vis) spectra showed a peak of surface plasmon resonance at 424 nm λmax, which corresponded to AgNP formation. The Fourier transform infrared spectroscopy (FT-IR) confirmed that biological moieties are involved for the development of AgNPs. Moreover, transmission electron microscopy (TEM) analyses confirmed the spherical shape and uniform size (8.11 to 15 nm) of the AgNPs. In human lung cancer cells (A549), the anticancer potential of AgNPs was examined by the MTT [3-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, scratch assay, and invasion assay. The results indicated that AgNPs inhibit the migration of A549 cells. The synthesized AgNPs showed MIC values of 12.5 µg/mL against Chromobacterium violaceum (C. violaceum) and 25 µg/mL against Pseudomonas aeruginosa (P. aeruginosa), which demonstrated their antibacterial abilities. Biological compounds that disable the QS system are being investigated as potential strategies for preventing bacterial infections. Thus, we analyzed the potential effectiveness of synthesized AgNPs in inhibiting QS-regulated virulence factors and biofilm formation in both strains of bacteria. In C. violaceum, the synthesized AgNPs significantly inhibited both violacein (85.18% at 1/2 × MIC) and acyl homoserine lactone (78.76% at 1/2 × MIC). QS inhibitory activity was also demonstrated in P. aeruginosa at a sub-MIC concentration (1/2 × MIC) by a reduction in pyocyanin activity (68.83%), total protease (68.50%), LasA activity (63.91%), and LasB activity (56.40%). Additionally, the exopolysaccharide production was significantly reduced in both C. violaceum (65.79% at 1/2 × MIC) and P. aeruginosa (57.65% at 1/2 × MIC). The formation of biofilm was also significantly inhibited at 1/2 × MIC in C. violaceum (76.49%) and in P. aeruginosa (65.31%). Moreover, a GC–MS analysis confirmed the presence of different classes of bioactive phytochemical constituents present in the leaf extract of E. sativa. On the basis of our results, we conclude that biologically synthesized AgNPs showed numerous multifunctional properties and have the potential to be used against human cancer and bacterial biofilm-related infections.
Dramatic Decrease of Vitamin K2 Subtype Menaquinone-7 in COVID-19 Patients
Authors: Mangge Harald , Prueller Florian , Dawczynski Christine , Curcic Pero , Sloup Zdenka , Holter Magdalena , Herrmann Markus , Meinitzer Andreas
Published: June 2022

(1) Background: Vitamin K (VK) is a fat-soluble compound with a common chemical structure, a 2-methyl-1,4-naphthoquinone ring, and a variable aliphatic side-chain. VK is involved in the synthesis of blood-clotting proteins, bone stability, anti-oxidative, and immune inflammatory-modulatory functions. Vitamin K also activates protein S, which acts as an antioxidant and anti-inflammatory. The fact that cytokine overproduction, oxidative stress, and disturbed microcirculation by thrombogenicity play a central role in severe COVID-19 prompted us to analyze this vitamin. (2) Methods: We analyzed by a validated liquid-chromatography tandem mass-spectrometry method serum vitamin K1, MK4, MK7, and VK epoxide levels in 104 healthy controls, 77 patients with non-COVID-19 pneumonia, and 135 hospitalized COVID-19 patients with potentially fatal outcomes admitted to our University Hospital between April and November 2020. We included the quotient between VK and triglyceride (TG, nmol/mmol/L) values in the analyses with respect to the TG transporter function for all VK subtypes. Additionally, we assessed anthropometric, routine laboratory, and clinical data from the laboratory and hospital information systems. (3) Results: The COVID-19 patients had significantly lower MK7 levels than non-COVID-19 pneumonia patients and healthy controls. COVID-19 and non-COVID-19 pneumonia patients had significantly lower vitamin K1 and significantly higher MK4 compared to healthy controls, but did not differ significantly from each other. Between COVID-19 non-survivors (n = 30) and survivors (n = 105) no significant differences were seen in all vitamin K subtypes, despite the fact that non-survivors had higher peak concentrations of IL-6, CRP, d-dimer, and higher oxygen needs, respectively. (4) Conclusions: The present data identified significantly decreased vitamin K1, K2 (MK7), and increased MK4 levels in patients with COVID-19 compared to healthy controls. Vitamin K2 (MK7) was lowest in COVID-19 patients irrespective of potentially fatal courses, indicating consumption of this VK subtype by COVID-19 immanent effects, most probably inflammatory and oxidative stress factors.
Deciphering the Path of S-nitrosation of Human Thioredoxin: Evidence of an Internal NO Transfer and Implication for the Cellular Responses to NO
Authors: Almeida Vitor S. , Miller Lara L. , Delia João P. G. , Magalhães Augusto V. , Caruso Icaro P. , Iqbal Anwar , Almeida Fabio C. L.
Published: June 2022

Nitric oxide (NO) is a free radical with a signaling capacity. Its cellular functions are achieved mainly through S-nitrosation where thioredoxin (hTrx) is pivotal in the S-transnitrosation to specific cellular targets. In this study, we use NMR spectroscopy and mass spectrometry to follow the mechanism of S-(trans)nitrosation of hTrx. We describe a site-specific path for S-nitrosation by measuring the reactivity of each of the 5 cysteines of hTrx using cysteine mutants. We showed the interdependence of the three cysteines in the nitrosative site. C73 is the most reactive and is responsible for all S-transnitrosation to other cellular targets. We observed NO internal transfers leading to C62 S-nitrosation, which serves as a storage site for NO. C69-SNO only forms under nitrosative stress, leading to hTrx nuclear translocation.
Lactobacillus plantarum Ameliorates High-Carbohydrate Diet-Induced Hepatic Lipid Accumulation and Oxidative Stress by Upregulating Uridine Synthesis
Authors: Xu Rong , Wang Tong , Ding Fei-Fei , Zhou Nan-Nan , Qiao Fang , Chen Li-Qiao , Du Zhen-Yu , Zhang Mei-Ling
Published: June 2022

The overconsumption of carbohydrates induces oxidative stress and lipid accumulation in the liver, which can be alleviated by modulation of intestinal microbiota; however, the underlying mechanism remains unclear. Here, we demonstrated that a strain affiliated with Lactobacillus plantarum (designed as MR1) efficiently attenuated lipid deposition, oxidative stress, as well as inflammatory response, which are caused by high-carbohydrate diet (HC) in fish with poor utilization ability of carbohydrates. Serum untargeted metabolome analysis indicated that pyrimidine metabolism was the significantly changed pathway among the groups. In addition, the content of serum uridine was significantly decreased in the HC group compared with the control group, while it increased by supplementation with L. plantarum MR1. Further analysis showed that addition of L. plantarum MR1 reshaped the composition of gut microbiota and increased the content of intestinal acetate. In vitro experiment showed that sodium acetate could induce the synthesis of uridine in hepatocytes. Furthermore, we proved that uridine could directly ameliorate oxidative stress and decrease liver lipid accumulation in the hepatocytes. In conclusion, this study indicated that probiotic L. plantarum MR1 ameliorated high-carbohydrate diet-induced hepatic lipid accumulation and oxidative stress by increasing the circulating uridine, suggesting that intestinal microbiota can regulate the metabolism of nucleotides to maintain host physiological homeostasis.
Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells
Authors: Herrera-Bravo Jesús , Beltrán Jorge F. , Huard Nolberto , Saavedra Kathleen , Saavedra Nicolás , Alvear Marysol , Lanas Fernando , Salazar Luis A.
Published: June 2022

Grape pomace is a source of anthocyanins, which can prevent cardiovascular diseases due to their antioxidant properties. Anthocyanin activity is associated with the ability to regulate oxidative stress through the transcription factor Nrf2. Thus, the present study aimed to evaluate if the anthocyanins found in Pinot noir pomace extract can affect the target genes related to the Nrf2 signalling pathway in endothelial cells. Our results highlight that the predominant anthocyanin in the Pinot noir pomace extract was malvidin-3-glucoside (3.7 ± 2.7 Eq. Malv-3-glu/kg). Molecular docking indicated that cyanidin-3-glucoside (−6.9 kcal/mol), malvidin-3-glucoside (−6.6 kcal/mol) and peonidin-3-glucoside (−6.6 kcal/mol) showed the highest affinities for the binding sites of the BTB domains in Keap1, suggesting that these components may modify the interaction of this protein with Nrf2. In addition, when HUVEC cells were exposed to different concentrations of Pinot noir pomace extract (100 µg/mL, 200 µg/mL, and 400 µg/mL), no changes in Nrf2 gene expression were observed. However, the gene expression of HO-1 and NQO1, which are in the signalling pathway of this transcription factor, increased according the concentrations of the extract (p = 0.0004 and p = 0.0084, respectively). In summary, our results show that anthocyanins play a very important role in Nrf2 activation and release, while at the same time not promoting its transcription. These preliminary results strongly suggest that the Pinot noir pomace extract can serve as a potent bioactive component source that protects cells against oxidative stress.
Endoplasmic Reticulum Stress and Reactive Oxygen Species in Plants
Authors: Cao Jiajian , Wang Chunhua , Hao Ning , Fujiwara Toru , Wu Tao
Published: June 2022

The endoplasmic reticulum (ER) is a key compartment responsible for protein processing and folding, and it also participates in many signal transduction and metabolic processes. Reactive oxygen species (ROS) are important signaling messengers involved in the redox equilibrium and stress response. A number of abiotic and biotic stresses can trigger the accumulation of unfolded or misfolded proteins and lead to ER stress. In recent years, a number of studies have reported that redox metabolism and ROS are closely related to ER stress. ER stress can benefit ROS generation and even cause oxidative burden in plants, finally leading to oxidative stress depending on the degree of ER stress. Moreover, ER stress activates nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-mediated ROS signaling, increases antioxidant defense mechanisms, and alters the glutathione (GSH) redox state. Meanwhile, the accumulation of ROS plays a special role in inducing the ER stress response. Given these factors, plants have evolved a series of complex regulatory mechanisms to interact with ROS in response to ER stress. In this review, we summarize the perceptions and responses of plant ER stress and oxidative protein folding in the ER. In addition, we analyze the production and signaling of ROS under ER stress in detail in order to provide a theoretical basis for reducing ER stress to improve the crop survival rate in agricultural applications.
Estimation of Redox Status in Military Pilots during Hypoxic Flight-Simulation Conditions—A Pilot Study
Authors: Petraki Konstantina , Grammatikopoulou Maria G. , Tekos Fotios , Skaperda Zoi , Orfanou Marina , Mesnage Robin , Vassilakou Tonia , Kouretas Demetrios
Published: June 2022

At high altitude conditions, the low-pressure atmospheric oxygen reduces the generation of energy, thus inducing a decrease in oxygen availability. As a result, endurance flights evoke imbalance in redox signaling, posing a safety risk for the pilots involved. The aim of the present study was to assess changes in the redox status of military pilots during flight simulation conditions according to their flight hours (experts vs. novice). A total of seven expert pilots and an equal number of novice pilots (trainees) were recruited from the Center for Airforce Medicine of the Greek Military Airforce. Glutathione (GSH) levels, catalase activity (CAT), total antioxidant capacity (TAC), lipid peroxidation through the thiobarbituric acid-reactive substances (TBARS), and protein oxidative damage through the assay of protein carbonyls (PCs) levels were assessed at two time points, once prior to and once immediately post a scheduled flight simulation. In the experienced pilots’ arms, GSH was significantly increased post-flight simulation, with TAC being simultaneously reduced. On the other hand, in the trainees’ arms, CAT and TAC were both increased post-flight. No differences were noted with regard to the TBARS and PCs post-simulation. When the two groups were compared, TAC and PCs were significantly lower in the trainees compared to the experienced pilots. The present study provides useful insight into the physiological redox status adaptations to hypobaric hypoxic flight conditions among pilots. In a further detail, an increase in GSH response post-flight simulation is being evoked in more experienced pilots, indicating an adaptation to the extreme flight conditions, as they battle oxidative stress.
Antibacterial and Antioxidant Activity of the Fruit of Macaranga tanarius, the Plant Origin of Taiwanese Green Propolis
Authors: Chien Yi-Hsuan , Yu Yu-Hsiang , Ye Siou-Ru , Chen Yue-Wen
Published: June 2022

Taiwanese green propolis (TGP) is widely used in traditional medicine and exerts a broad spectrum of biological activities, including those anti-inflammatory and anti-cancer in nature, resulting from an abundant level of functional propolins (prenylated flavanone) in the TGP. However, the plant origin of TGP has not been clarified. In this study, we collected the surface material of Macaranga tanarius fruit and comparatively analyzed the chemical composition, antibacterial activity, and antioxidant activity with TGP. The results revealed that there was no difference between the chemical composition of the glandular trichome extract of M. tanarius and those in propolis. Moreover, M. tanarius fruit extract was enriched in propolins (C, D, F, and G) and effectively inhibited the growth of Gram-positive strains. Propolins, TGP, and M. tanarius fruit extract showed powerful free radical-scavenging and ferrous-reducing activity. Collectively, we have confirmed the plant source of TGP is M. tanarius, and this plant has the enormous potential to be developed as a pharmaceutical plant due to the potent biological activities and the high amount of functional propolins.
Differences in Antioxidant Potential of Allium cepa Husk of Red, Yellow, and White Varieties
Authors: Chernukha Irina , Kupaeva Nadezhda , Kotenkova Elena , Khvostov Daniil
Published: June 2022

The effective management of agro-industry organic waste for developing high-commercial-value products is a promising facet of the circular economy. Annually, more than 550,000 tons of waste that is potentially rich in biologically active substances is generated worldwide while processing onions (Allium cepa L.). The antioxidant potential of red, yellow, and white onion husks was studied using FRAP, ORAC, chemiluminescence, and UPLC-ESI-Q-TOF-MS analysis methods. The extraction of phenolic compounds from onion husks was more effective when using an aqueous solution of 70% ethanol as compared with water. Ethanolic extract from red onion husks exhibited the highest TACORAC and TACFRAP values, averaging 2017.34 µmol-equiv. Trolox/g raw material and 2050.23 µmol-equiv. DQ/g raw material, respectively, while the white onion exhibited much lower levels of antioxidants. According to the chemiluminescence results, it was determined that the red and yellow onion husks contained antioxidants of three types of power, while white onion husks only contained medium and weak types. The highest content of flavonoids was found in red onion husks, averaging 1915.90 ± 9.92 µg-eq. DQ/g of raw material and 321.42 ± 2.61 µg-eq. DQ/g of raw material for ethanol and water, respectively, while yellow onion husks exhibited 544.06 ± 2.73 µg-eq. DQ/g of raw material and 89.41 ± 2.08 for ethanol and water, respectively. Quercetin and its glycosides were the most representative flavonoids, and a number of substances with different pharmacological and biological properties were also identified.
Neuroprotective Profile of Edible Flowers of Borage (Borago officinalis L.) in Two Different Models: Caenorhabditis elegans and Neuro-2a Cells
Authors: Moliner Cristina , Cásedas Guillermo , Barros Lillian , Finimundy Tiane C. , Gómez-Rincón Carlota , López Víctor
Published: June 2022

The flowers of Borago officinalis L. (Boraginaceae), commonly known as borage, are widely used as a culinary ingredient. The aim of this study was to assess the potential benefits of fresh borage flower extract related to antioxidant, neuroprotective and anti-aging properties. The extract was obtained by Soxhlet extraction with ethanol as a solvent, and fatty acids were detected by GC-FID. The antioxidant activity was evaluated in vitro through the DPPH, FRAP and ORAC assays. Regarding the fatty acid (FA) composition, the extract showed high amounts of polyunsaturated FA. The Neuro-2a cell line was used to determine the cytoprotective capacity of the extract subjected to oxidative stress (H2O2). Moreover, the model organism Caenorhabditis elegans was used to assess antioxidant activity, delayed ageing as well as cytoprotection and reduced β-amyloid toxicity. Cells treated with the extract and H2O2 showed a better response to oxidative stress than the control group, particularly in terms of mitochondrial activity (MTT assay), redox state (ROS formation) and the activity of antioxidant enzymes (catalase and superoxide dismutase). B. officinalis flower extract showed promising antioxidant activity in the selected models, without causing toxicity. Hence, the results obtained support the antioxidant properties of borage flowers in different bioassays using living organisms.
Novel Probiotic Lactic Acid Bacteria Were Identified from Healthy Infant Feces and Exhibited Anti-Inflammatory Capacities
Authors: Li Binbin , Pan Li-Long , Sun Jia
Published: June 2022

The current study aims to evaluate the probiotic potential of lactic acid bacteria isolated from infant feces, and select candidates to be used as potential antioxidants for the treatment of oxidative stress-related diseases; To meet the criteria for probiotic attributes, the isolates were subjected to various in vitro tests and 16S rRNA genotypic characterization. Besides, anti-inflammatory and anti-oxidative effects of selected isolates were separately assessed by real-time quantitative PCR and Western blot; The selected strains belonged to Lactobacillus gasseri, Lactiplantibacillus plantarum and Lacticaseibacillus rhamnosus. Notably, three selected strains (L. gasseri FWJL-4, L. plantarum Fjias-5 and L. rhamnosus FSJ-13) particularly L. gasseri FWJL-4 significantly down-regulated mRNA expression levels of tumor necrosis factor α (TNFα), Interleukin-6 (IL-6) and IL-1β. Most importantly, three strains-treated RAW 264.7 murine macrophages displayed enhanced activities of antioxidant enzymes and reduced H2O2 production, which were associated with the enhanced expression levels of nuclear factor-erythroid 2 related factor 2 and heme oxygenase-1; Three selected strains, particularly L. gasseri FWJL-4, are good candidates that merit additional in vivo investigation for the validation and application of their health-promoting effects.
The Variable Nature of Vitamin C—Does It Help When Dealing with Coronavirus?
Authors: Grudlewska-Buda Katarzyna , Wiktorczyk-Kapischke Natalia , Budzyńska Anna , Kwiecińska-Piróg Joanna , Przekwas Jana , Kijewska Agnieszka , Sabiniarz Dominika , Gospodarek-Komkowska Eugenia , Skowron Krzysztof
Published: June 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still spreading worldwide. For this reason, new treatment methods are constantly being researched. Consequently, new and already-known preparations are being investigated to potentially reduce the severe course of coronavirus disease 2019 (COVID-19). SARS-CoV-2 infection induces the production of pro-inflammatory cytokines and acute serum biomarkers in the host organism. In addition to antiviral drugs, there are other substances being used in the treatment of COVID-19, e.g., those with antioxidant properties, such as vitamin C (VC). Exciting aspects of the use of VC in antiviral therapy are its antioxidant and pro-oxidative abilities. In this review, we summarized both the positive effects of using VC in treating infections caused by SARS-CoV-2 in the light of the available research. We have tried to answer the question as to whether the use of high doses of VC brings the expected benefits in the treatment of COVID-19 and whether such treatment is the correct therapeutic choice. Each case requires individual assessment to determine whether the positives outweigh the negatives, especially in the light of populational studies concerning the genetic differentiation of genes encoding the solute carriers responsible forVC adsorption. Few data are available on the influence of VC on the course of SARS-CoV-2 infection. Deducing from already-published data, high-dose intravenous vitamin C (HDIVC) does not significantly lower the mortality or length of hospitalization. However, some data prove, among other things, its impact on the serum levels of inflammatory markers. Finally, the non-positive effect of VC administration is mainly neutral, but the negative effect is that it can result in urinary stones or nephropathies.
The Use of Quercetin to Improve the Antioxidant and Regenerative Properties of Frozen or Cryopreserved Human Amniotic Membrane
Authors: Purpura Valeria , Benedetti Serena , Bondioli Elena , Scarpellini Francesca , Giacometti Agnese , Albertini Maria Cristina , Melandri Davide
Published: June 2022

The biological properties of the human amniotic membrane (HAM) and its characteristic ability to be a reservoir of growth factors promoting wound healing make it an ideal biological dressing for the treatment of different clinical conditions, such as burns and non-healing wounds. However, the application of a preservation method on the HAM is required during banking to maintain biological tissue properties and to ensure the release overtime of protein content for its final clinical effectiveness after application on the wound bed. Although cryopreservation and freezing are methods widely used to maintain tissue properties, reactive oxygen species (ROS) are produced within tissue cellular components during their switching from frozen to thawed state. Consequently, these methods can lead to oxidative stress-induced cell injury, affecting tissue regenerative properties and its final clinical effectiveness. Taking advantage of the antioxidant activity of the natural compound quercetin, we used it to improve the antioxidant and regenerative properties of frozen or cryopreserved HAM tissues. In particular, we evaluated the oxidative damage (lipid peroxidation, malondialdehyde) as well as the regenerative/biological properties (bFGF growth factor release, wound healing closure, structure, and viability) of HAM tissue after its application. We identified the effectiveness of quercetin on both preservation methods to reduce oxidative damage, as well as its ability to enhance regenerative properties, while maintaining the unaltered structure and viability of HAM tissue. The use of quercetin described in this study appears able to counteract the side effects of cryopreservation and freezing methods related to oxidative stress, enhancing the regenerative properties of HAM. However, further investigations will need to be performed, starting from these promising results, to identify its beneficial effect when applied on burns or non-healing wounds.
Sinapicacid Inhibits Group IIA Secretory Phospholipase A2 and Its Inflammatory Response in Mice
Authors: Giresha Aladahalli S. , Urs Deepadarshan , Pundalik Sophiya , Meti Rajkumar S. , Pramod Siddanakoppalu N. , Supreetha Ballenahalli H. , Somegowda Madhusudana , Dharmappa Kattepura K. , El-Shehawi Ahmed M. , Albogami Sarah , Elseehy Mona M. , Alaklabi Abdullah , Elansary Hosam O. , Mehder Alanoud Omur A. , Mahmoud Eman A.
Published: June 2022

Human Group IIA secreted phospholipase A2 (sPLA2-IIA) enzyme plays a crucial role in several chronic inflammatory diseases such asasthma, atherosclerosis, gout, bronchitis, etc. Several studies showed that the antioxidants exert an anti-inflammatory function by inhibiting the sPLA2-IIA enzyme. Hence, the present study evaluated an antioxidant molecule, sinapic acid, for sPLA2-IIA inhibition as an anti-inflammatory function. Initially, the antioxidant efficacy of sinapic acid was evaluated, and it showed greater antioxidant potency. Further, sinapic acid inhibited 94.4 ± 4.83% of sPLA2-IIA activity with an IC50 value of 4.16 ± 0.13 µM. The mode of sPLA2-IIA inhibition was examined by increasing the substrate concentration from 30 to 120nM and the calcium concentration from 2.5 to 15 mM, which did not change the level of inhibition. Further, sinapic acid altered the intrinsic fluorescence and distorted the far UltraViolet Circular Dichroism (UV-CD) spectra of the sPLA2-IIA, indicating the direct enzyme-inhibitor interaction. Sinapic acid reduced the sPLA2-IIA mediated hemolytic activity from 94 ± 2.19% to 12.35 ± 2.57% and mouse paw edema from 171.75 ± 2.2% to 114.8 ± 1.98%, demonstrating the anti-inflammatory efficiency of sinapic acid by in situ and in vivo methods, respectively. Finally, sinapic acid reduced the hemorrhagic effect of Vipera russelli venom hemorrhagic complex-I (VR-HC-I) as an anti-hemorrhagic function. Thus, the above experimental results revealed the sinapic acid potency to be an antioxidant, anti-inflammatory and anti-hemorrhagic molecule, and therefore, it appears to be a promising therapeutic agent.
Ambivalent Roles of Oxidative Stress in Triangular Relationships among Arthropod Vectors, Pathogens and Hosts
Authors: Hernandez Emmanuel Pacia , Anisuzzaman  , Alim Md Abdul , Kawada Hayato , Kwofie Kofi Dadzie , Ladzekpo Danielle , Koike Yuki , Inoue Takahiro , Sasaki Sana , Mikami Fusako , Matsubayashi Makoto , Tanaka Tetsuya , Tsuji Naotoshi , Hatta Takeshi
Published: June 2022

Blood-feeding arthropods, particularly ticks and mosquitoes are considered the most important vectors of arthropod-borne diseases affecting humans and animals. While feeding on blood meals, arthropods are exposed to high levels of reactive oxygen species (ROS) since heme and other blood components can induce oxidative stress. Different ROS have important roles in interactions among the pathogens, vectors, and hosts. ROS influence various metabolic processes of the arthropods and some have detrimental effects. In this review, we investigate the various roles of ROS in these arthropods, including their innate immunity and the homeostasis of their microbiomes, that is, how ROS are utilized to maintain the balance between the natural microbiota and potential pathogens. We elucidate the mechanism of how ROS are utilized to fight off invading pathogens and how the arthropod-borne pathogens use the arthropods’ antioxidant mechanism to defend against these ROS attacks and their possible impact on their vector potentials or their ability to acquire and transmit pathogens. In addition, we describe the possible roles of ROS in chemical insecticide/acaricide activity and/or in the development of resistance. Overall, this underscores the importance of the antioxidant system as a potential target for the control of arthropod and arthropod-borne pathogens.
hLSTM-Aging: A Hybrid LSTM Model for Software Aging Forecast
Authors: Battisti Felipe , Silva Arnaldo , Pereira Luis , Carvalho Tiago , Araujo Jean , Choi Eunmi , Nguyen Tuan Anh , Min Dugki
Published: June 2022

Long-running software, such as cloud computing services, is now widely used in modern applications. As a result, the demand for high availability and performance has grown. However, these applications are more vulnerable to software aging issues and are more likely to fail due to the accumulation of mistakes in the system. One popular strategy for dealing with such aging-related problems is to plan prediction-based software rejuvenation activities based on previously obtained data from long-running software. Prediction algorithms enable the activation of a mitigation mechanism before the problem occurs. The long short-term memory (LSTM) neural network, the present state of the art in temporal series prediction, has demonstrated promising results when applied to software aging concerns. This study aims to anticipate software aging failures using a hybrid prediction model integrating long short-term memory models and statistical approaches. We emphasize the capabilities of each strategy in various long-running software scenarios and provide an untried hybrid model (hLSTM-aging) based on the union of Conv-LSTM networks and probabilistic methodologies, attempting to combine the strengths of both approaches for software aging forecasts. The hLSTM-aging prediction results revealed how hybrid models are a compelling solution for software-aging prediction. Experiments showed that hLSTM-aging increased MSE criteria by 8.54% to 50% and MAE criteria by 3.53% to 14.29% when compared to Conv-LSTM, boosting the model’s initial performance.
Bio_Fabricated Levan Polymer from Bacillus subtilis MZ292983.1 with Antibacterial, Antibiofilm, and Burn Healing Properties
Authors: Hamada Marwa A. , Hassan Rasha A. , Abdou Amr M. , Elsaba Yasmin M. , Aloufi Abeer S. , Sonbol Hana , Korany Shereen M.
Published: June 2022

The biopolymer levan has sparked a lot of interest in commercial production and various industrial applications. In this study, a bacterial isolate with promising levan-producing ability was isolated from a soil sample obtained from Princess Nourah bint Abdulrahman University in Saudi Arabia. The isolate has been identified and submitted to GenBank as Bacillus subtilis MZ292983.1. The bacterial levan polymer was extracted using ethyl alcohol (75%) and CaCl2 (1%) and then characterized using several approaches, such as Fourier transform infrared spectrometry and nuclear magnetic resonance. The IR spectrum of the levan polymer showed characteristic peaks confirming characteristics of polysaccharides, including a broad stretching peak of OH around 3417 cm−1 and aliphatic CH stretching was observed as two peaks at 2943, and 2885 cm−1. In addition, the FTIR spectrum featured an absorption at 2121 cm−1, indicating the fingerprint of the β-glycosidic bond. Based on 1H and 13C NMR spectroscopy analysis, six unexchanged proton signals related to fructose as a forming monomer of levan were observed. Evaluation of levan’s antibacterial effect against two pathogenic bacteria, S. aureus (ATCC 33592) and E. coli (ATCC 25922), showed inhibition zones of 1 cm and 0.8 cm in diameter, respectively, with MICs of more than 256 μg mL−1 for both strains. Moreover, the antibiofilm property of the levan polymer was assessed and the results showed that the inhibition rate was positively proportional to the levan concentration, as the inhibition percentages were 50%, 29.4%, 29.4%, 26.5%, and 14.7% at concentrations of 2, 1, 0.5, 0.25, and 0.125 mg mL−1, respectively. Levan showed a significant role in burn healing properties since it accelerated the process of healing burn-induced areas in rats when compared with those either treated with normal saline or treated with the cream base only.
Medical Gesture Recognition Method Based on Improved Lightweight Network
Authors: Wang Wenjie , He Mengling , Wang Xiaohua , Ma Jianwei , Song Huajian
Published: June 2022

Surgery is a compelling application field for collaborative control robots. This paper proposes a gesture recognition method applied to a medical assistant robot delivering instruments to collaborate with surgeons to complete surgeries. The key to assisting the surgeon in passing instruments in the operating room is the ability to recognize the surgeon’s hand gestures accurately and quickly. Existing gesture recognition techniques suffer from poor recognition accuracy and low rate. To address the existing shortcomings, we propose an improved lightweight convolutional neural network called E-MobileNetv2. The ECA module is added to the original MobileNetv2 network model to obtain more useful features by computing the information interactions between the current channel and the adjacent channels and between the current channel and the distant channels in the feature map. We add R6-SELU activation function to enhance the network’s ability to extract features. By adjusting the shrinkable hyper-parameters, the number of parameters of the network is reduced to improve the recognition speed. The improved network model achieves excellent performance on both the self-built dataset Gesture_II and the public dataset Jester. The recognition accuracy of the improved model is 96.82%, which is 3.17 % higher than that of the original model, achieving an increase in accuracy and recognition speed.
A Variable-Order Dynamic Constitutive Model for Clay Based on the Fractional Calculus
Authors: Zhang Bo-Lang , Chen Kai-Sheng , Hu Xing , Zhang Kun
Published: June 2022

To accurately describe the deformation characteristics of clay under long-term cyclic load, based on fractional calculus theory, elastoplastic theory and the basic element model, a variable-order fractional dynamic model designed to predict accumulative strain of clay was exhibited. Firstly, the cyclic load was separated into static and alternating load in accordance with cyclic load characteristics, and the strain of clay under static and alternating load was analyzed. Then, on the basis of the variable-order Abel dashpot model, rheological theory and elastoplastic theory, the expressions of the rheological constitutive model and strain response were both obtained. Finally, in combination with the undrained dynamic triaxial testing of Zhan Jiang clay and Tian Jin soft clay, a series of analyses was carried out on the effectiveness and parameter sensitivity of the model when subjected to long-term cyclic loading. By comparing the dynamic constitutive model with pre-existing models, the superiority of the dynamic constitutive model is revealed. The results show that the dynamic constitutive model can characterize properly the deformation characteristics of clay under the action of long-term cyclic loading, especially in its accelerating stage. The parameter sensitivity of the model exhibits a growing trend with the increment of loading duration.
Facial Expressions Based Automatic Pain Assessment System
Authors: Alghamdi Thoria , Alaghband Gita
Published: June 2022

Pain assessment is used to improve patients’ treatment outcomes. Human observers may be influenced by personal factors, such as inexperience and medical organizations are facing a shortage of experts. In this study, we developed a facial expressions-based automatic pain assessment system (FEAPAS) to notify medical staff when a patient suffers pain by activating an alarm and recording the incident and pain level with the date and time. The model consists of two identical concurrent subsystems, each of which takes one of the two inputs of the model, i.e., “full face” and “the upper half of the same face”. The subsystems extract the relevant input features via two pre-trained convolutional neural networks (CNNs), using either VGG16, InceptionV3, ResNet50, or ResNeXt50, while freezing all convolutional blocks and replacing the classifier layer with a shallow CNN. The concatenated outputs in this stage is then sent to the model’s classifier. This approach mimics the human observer method and gives more importance to the upper part of the face, which is similar to the Prkachin and Soloman pain intensity (PSPI). Additionally, we further optimized our models by applying four optimizers (SGD/ADAM/RMSprop/RAdam) to each model and testing them on the UNBC-McMaster shoulder pain expression archive dataset to find the optimal combination, InceptionV3-SGD. The optimal model showed an accuracy of 99.10% on 10-fold cross-validation, thus outperforming the state-of-the-art model on the UNBC-McMaster database. It also scored 90.56% on unseen subject data. To speed up the system response time and reduce unnecessary alarms associated with temporary facial expressions, a select but effective subset of frames was inspected and classified. Two frame-selection criteria were reported. Classifying only two frames at the middle of 30-frame sequence was optimal, with an average reaction time of at most 6.49 s and the ability to avoid unnecessary alarms.
Optimizing IoT Web Fuzzing by Firmware Infomation Mining
Authors: Gao Yifei , Zhou Xu , Xie Wei , Wang Baosheng , Wang Enze , Wang Zhenhua
Published: June 2022

IoT web fuzzing is an effective way to detect security flaws in IoT devices. However, without enough information of the tested targets, IoT web fuzzing is often blind and inefficient. In this paper, we propose to use static analysis to assist IoT web fuzzing. Our insight is that plenty of useful information is hidden in firmwares, which can be mined by static analysis and used to guide the subsequent dynamic analysis—fuzzing. Hence, our approach contains two stages: pre-fuzzing stage and fuzzing stage. In the pre-fuzzing stage, we perform static analysis on the IoT firmwares to exploit helpful information, such as web page paths, interfaces, and shared keywords. These kinds of information are used to construct diverse seeds for covering more web paths and interfaces, and are also used to prioritize seeds according to their importance (related to shared keywords) in the fuzzing stage. Based on this approach, we implement a prototype IoT web fuzzing system—IoTParser. Experiments show that IoTParser increased the vulnerability discovery capability by 44% on average, while increasing the vulnerability discovery efficiency by 48.2% on average compared with state-of-the-art IoT web fuzzer. In addition, IoTParser has found 13 vulnerabilities, including 7 0-day.
The Challenge of Long-Distance Over-the-Air Wireless Links in the Ocean: A Survey on Water-to-Water and Water-to-Land MIoT Communication
Authors: Dinis Hugo , Rocha João , Matos Tiago , Gonçalves Luís M. , Martins Marcos
Published: June 2022

Robust wireless communication networks are a cornerstone of the modern world, allowing data to be transferred quickly and reliably. Establishing such a network at sea, a Maritime Internet of Things (MIoT), would enhance services related to safety and security at sea, environmental protection, and research. However, given the remote and harsh nature of the sea, installing robust wireless communication networks with adequate data rates and low cost is a difficult endeavor. This paper reviews recent MIoT systems developed and deployed by researchers and engineers over the past few years. It contains an analysis of short-range and long-range over-the-air radio-frequency wireless communication protocols and the synergy between these two in the pursuit of an MIoT. The goal of this paper is to serve as a go-to guide for engineers and researchers that need to implement a wireless sensor network at sea. The selection criterion for the papers included in this review was that the implemented wireless communication networks were tested in a real-world scenario.