bluuebunny/crossref_metadata_2025 huggingface dataset:
The Uyghur Minority in China: A Case Study of Cultural Genocide, Minority Rights and the Insufficiency of the International Legal Framework in Preventing State-Imposed Extinction
Authors: Finnegan Ciara
Published: January 2020

Raphael Lemkin, the man who founded the term ‘genocide,’ did so with a view to protecting not only physical beings from systematically imposed extinction, but also protecting their cultures from the same fate. However, in the wake of the atrocities and bloodshed of WWII, cultural genocide was omitted from the 1948 Genocide Convention, and as a result, does not constitute an international crime. This omission has left a lacuna in international law which threatens minority groups. Not a threat of loss of life but rather loss of the culture that distinguishes them and identifies them as a minority. Powerful States with indifferent attitudes towards their international obligations face no significantly harsher punishment for cultural genocide than they do for other human rights transgressions. Consequently, cultural genocide continues as minority cultures are rendered extinct at the hands of States. The Case Study of this article investigates the present-day example of the Uyghur minority in China and analyzes whether this modern cultural genocide can pave the way for the recognition of cultural genocide as an international crime or whether the Uyghur culture will become a cautionary tale for minorities in the future.
The Effect of Ultrasonic Cleaning on the Secondary Electron Yield, Surface Topography, and Surface Chemistry of Laser Treated Aluminum Alloy
Authors: Wang Jie , Gao Yong , You Zhiming , Fan Jiakun , Zhang Jing , Wang Sheng , Xu Zhanglian
Published: January 2020

Laser ablation technique is a novel method for obtaining a surface with a low secondary electron yield (SEY) that can mitigate electron cloud in high-energy accelerators. Before the installation of laser processed aluminum alloy, surface cleaning is of the essence to reduce the contaminations of ultra-high vacuum systems for providing appropriate pressure for beam operation consequently. Laser processed aluminum alloy is one of the crucial candidates for the vacuum system construction of future accelerators. Moreover, ultrasonic cleaning is an essential procedure for most materials applied in vacuum systems. Therefore, in order to verify the stability of the laser created structures by ultrasonic cleaning and evaluate the impact of the cleaning on the SEYs, the surface topographies, and the surface chemistries of laser treated aluminum alloy, SEY measurements and related tests were performed. After ultrasonic cleaning, the SEYs of laser treated aluminum alloy increased from 0.99, 1.05, and 1.16 to 1.43, 1.74, and 1.38, respectively. Compared to the surface roughness of uncleaned laser treated aluminum samples, the cleaned laser treated ones decreased from 10.7, 7.5, and 14.5 to 9.4, 6.9, and 12.9, respectively. The results indicate that ultrasonic cleaning can induce the SEY increase of laser processed aluminum alloy. The correlative mechanism between the surface morphology, the surface chemistry, and SEY increase were analyzed for the first time.
Cell-Free Scaffolds as a Monotherapy for Focal Chondral Knee Defects
Authors: Kwan Haowen , Chisari Emanuele , Khan Wasim S.
Published: January 2020

Chondral knee defects have a limited ability to be repaired. Current surgical interventions have been unable to regenerate articular cartilage with the mechanical properties of native hyaline cartilage. The use of a scaffold-based approach is a potential solution. Scaffolds are often implanted with cells to stimulate cartilage regeneration, but cell-based therapies are associated with additional regulatory restrictions, an additional surgical procedure for cell harvest, time for cell expansion, and the associated costs. To overcome these disadvantages, cell-free scaffolds can be used in isolation allowing native cells to attach over time. This review discusses the optimal properties of scaffolds used for chondral defects, and the evidence for the use of hydrogel scaffolds and hydrogel–synthetic polymer hybrid scaffolds. Preclinical and clinical studies have shown that cell-free scaffolds can support articular cartilage regeneration and have the potential to treat chondral defects. However, there are very few studies in this area and, despite the many biomaterials tested in cell-based scaffolds, most cell-free studies focused on a specific type I collagen scaffold. Future studies on cell-free scaffolds should adopt the modifications made to cell-based scaffolds and replicate them in the clinical setting. More studies are also needed to understand the underlying mechanism of cell-free scaffolds.
Additional Porosity as a Side Effect of Polycarboxylate Addition and Its Influence on Concrete’s Scaling Resistance
Authors: Nowak-Michta Aneta
Published: January 2020

A side effect of using modified polycarboxylates to liquefy a concrete mix is additional pores in the concrete. They change the air void system in hardened concretes, and can be used to evaluate the freeze–thaw resistance of concretes. The purpose of this study is to determine the impact of the abovementioned quantitative and qualitative parameters on the freeze–thaw resistance of concretes. The research program was performed on eight sets of air-entraining and non-air-entraining concretes with a variable content of superplasticizer based on modified polycarboxylates. The basic composition of and air-entraining admixture content in the air-entraining concrete mixtures were held constant. Pore structure tests were performed according to EN 480-11. Scaling resistance was determined according to PKN-CEN/TS 12390-9. The results showed that as the content of modified polycarboxylates increased, the pore structure was adversely affected, and, consequently, the air void parameters deteriorated. At the same time, the freeze–thaw resistance of the non-air-entraining concretes decreased. The pores sizes also changed. As the fluidity increased, the specific surface area decreased, and, consequently, the spacing factor increased. The air-entraining concretes, despite the deterioration in the pore structure due to the modified polycarboxylates, were found to be very good quality concretes after 56 freeze–thaw cycles in the presence of 3% NaCl.
The Effect of Soil Mineral Composition on the Compressive Strength of Cement Stabilized Rammed Earth
Authors: Narloch Piotr , Woyciechowski Piotr , Kotowski Jakub , Gawriuczenkow Ireneusz , Wójcik Emilia
Published: January 2020

Cemented stabilized rammed earth (CSRE) is a building material used to build load bearing walls from locally available soil. The article analyzes the influence of soil mineral composition on CSRE compressive strength. Compression tests of CSRE samples of various mineral compositions, but the same particle size distribution, water content, and cement content were conducted. Based on the compression strength results and analyzed SEM images, it was observed that even small changes in the mineral composition significantly affected the CSRE compressive strength. From the comparison of CSRE compressive strength result sets, one can draw general qualitative conclusions that montmorillonite lowered the compressive strength the most; beidellite also lowered it, but to a lesser extent. Kaolinite lightly increased the compressive strength.
Configuration Optimisation of Laser Tracker Location on Verification Process
Authors: Aguado Sergio , Pérez Pablo , Albajez José Antonio , Santolaria Jorge , Velázquez Jesús
Published: January 2020

Machine tools are verified and compensated periodically to improve accuracy. The main aim of machine tool verification is to reduce the influence of quasi-static errors, especially geometric errors. As these errors show systematic behavior, their influence can be compensated. However, verification itself is influenced by random uncertainty sources that are usually not considered but affect the results. Within these uncertainty sources, laser tracker measurement noise is a random error that should not be ignored and can be reduced through adequate location of the equipment. This paper presents an algorithm able to analyse the influence of laser tracker location based on nonlinear optimisation, taking into consideration its specifications and machine tool characteristics. The developed algorithm uses the Monte Carlo method to provide a zone around the machine tool where the measurement system should be located in order to improve verification results. To achieve this aim, different parameters were defined, such as the number of tests carried out, and the number and distribution of points, and their influence on the error due to the laser tracker location analysed.
Limitation in Controlling the Morphology of Mammalian Vero Cells Induced by Cell Division on Asymmetric Tungsten-Silicon Oxide Nanocomposite
Authors: Moussa Hassan I. , Chan Wing Y. , Logan Megan , Aucoin Marc G. , Tsui Ting Y.
Published: January 2020

Engineered nanomaterials are often used in tissue engineering applications to influence and manipulate the behavior of cells. Recently, a number of tungsten-silicon oxide nanocomposite devices containing equal width (symmetric) tungsten and silicon oxide parallel line comb structures were developed and used by our group. The devices induced over 90% of seeded cells (Vero) to align within ±20° of the axes of 10 µm wide tungsten lines. Furthermore, a mathematical model was successfully developed to predict this alignment behavior and forecast the minimum width of isolated tungsten lines required to induce such behavior. However, the mechanism by which the widths of the symmetrical tungsten and silicon oxide lines induce the alignment behavior is still unknown. Furthermore, the model was never tested on more complex asymmetrical structures. Herewith, experiments were conducted with mammalian cells on complex asymmetrical structures with unequal tungsten and silicon oxide line widths. Results showed that the model could be extended to more complex pattern structures. In addition, cell morphology on the patterned structures reset during cell division because of mitotic rounding, which reduced the population of cells that elongated and aligned on the tungsten lines. Ultimately, we concluded that it was impossible to achieve a 100% alignment with cells having unsynchronized cell cycles because cell rounding during mitosis took precedence over cell alignment; in other words, internal chemical cues had a stronger role in cell morphology than external cues.
Effect of the Solution Temperature on the Precipitates and Grain Evolution of IN718 Fabricated by Laser Additive Manufacturing
Authors: Cao Yu , Bai Pucun , Liu Fei , Hou Xiaohu , Guo Yuhao
Published: January 2020

The effects of the solution heat treatment temperature on the precipitates, grain boundary evolution and response of the microhardness of Inconel 718 (IN718) superalloy fabricated by selective laser melting (SLM) were investigated. It was found that: (1) The long-chained Laves phases formed in the as-deposited condition dissolved into the matrix when the solution temperature rises above 980 °C. (2) The width-to-length ratio was maintained at approximately 1.6 when the solution was heated from 980 °C to 1080 °C, and dropped down to 1.03 when heated to 1130 °C. (3) Low-angle grain boundaries kept the same number fraction of 65% from 980 to 1080 °C as the as-deposited condition, and decreased dramatically from 1090 to 1130 °C to 4%. (4) Annealing twin boundaries occurred at 1090 °C with a number fraction of 3%, and quickly increased to 65% when heated to 1130 °C. It is concluded that the static recrystallization of IN718 fabricated by selective laser melting (SLM) occurred at 1090 °C and fast proceeded to full recrystallization at 1130 °C. The forming of annealing twins accompanies the recrystallization process and is an effective way to refine the recrystallized grain size.
Methodology for Revealing the Phases and Microstructural Constituents of the CMSX-4 Nickel-Based Superalloy Implicating Their Computer-Aided Detection for Image Analysis
Authors: Szczotok Agnieszka , Reichel Hannah
Published: January 2020

The paper presents the findings of a research on the selection of methodology for revealing the microstructure in metallographic investigations on the example of the single-crystalline CMSX-4 nickel-based superalloy. A set of chemical and electrochemical methods of etching has been selected. The metallographic specimens from the analyzed material have been treated with the etchants. After every etching procedure, microphotographs of the microstructure were taken by means of an optical microscope and a scanning electron microscope. Both useful and disadvantageous effects of etching with the respective etchants have been displayed. The etchant application for a qualitative and quantitative analysis has been considered on the basis of the enclosed microphotographs. As a result, examples of a computer-aided detection of the phases and microstructural constituents present in the analyzed CMSX-4 alloy for the selected revealing methodologies have been demonstrated. The described investigations enable a better understanding of the essence of the selection of the microstructure revealing methodology and its influence on the obtained results.
XRD and TG-DTA Study of New Alkali Activated Materials Based on Fly Ash with Sand and Glass Powder
Authors: Burduhos Nergis Dumitru Doru , Abdullah Mohd Mustafa Al Bakri , Sandu Andrei Victor , Vizureanu Petrică
Published: January 2020

In this paper, the effect on thermal behavior and compounds mineralogy of replacing different percentages of fly ash with compact particles was studied. A total of 30% of fly ash was replaced with mass powder glass (PG), 70% with mass natural aggregates (S), and 85% with mass PG and S. According to this study, the obtained fly ash based geopolymer exhibits a 20% mass loss in the 25–300 °C temperature range due to the free or physically bound water removal. However, the mass loss is closely related to the particle percentage. Multiple endothermic peaks exhibit the dihydroxylation of β-FeOOH (goethite) at close to 320 °C, the Ca(OH)2 (Portlandite) transformation to CaCO3 (calcite) occurs at close to 490 °C, and Al(OH)3 decomposition occurs at close to 570 °C. Moreover, above 600 °C, the curves show only very small peaks which may correspond to Ti or Mg hydroxides decomposition. Also, the X-ray diffraction (XRD) pattern confirms the presence of sodalite after fly ash alkaline activation, whose content highly depends on the compact particles percentage. These results highlight the thermal stability of geopolymers in the 25–1000 °C temperature range through the use of thermogravimetric analysis, differential thermal analysis, and XRD.
Grain Size-Related Strengthening and Softening of a Precompressed and Heat-Treated Mg–Zn–Ca Alloy
Authors: Dobroň Patrik , Drozdenko Daria , Horváth Fekete Klaudia , Olejňák Juraj , Bohlen Jan
Published: January 2020

The impact of precompression, thermal treatment and its combination on the deformation behaviour of an extruded Mg–Zn–Ca (ZX10) alloy was studied with respect to a varied average grain size. The Hall–Petch plot was used to highlight the impact in a wide grain size interval. The initial texture of the wrought alloy was characterized by X-ray diffraction. Moreover, the evolution of microstructure and texture was provided by the electron backscatter diffraction (EBSD) technique. The obtained results indicate the strong contribution of deformation-thermal treatment on the resulting deformation behaviour. Particularly, after precompression and heat treatment, higher strengthening effect was observed in the reversed tensile loaded compared to compressed samples without any change in the Hall–Petch slope throughout the grain size interval. Unlike this strengthening effect, a reversed tension–compression yield asymmetry with higher strength values in compression has been obtained.
Chloride Resistance of Portland Cement-Based Mortar Incorporating High Aluminate Cement and Calcium Carbonate
Authors: Lee Yunsu , Lim Seungmin , Lee Hanseung
Published: January 2020

Whether chloride resistance is highly influenced by chloride binding capacity remains unknown. In this study, the chloride resistance of Portland cement-based mortar incorporating aluminate cement and calcium carbonate was investigated considering the chloride binding capacity, pore structures and chloride diffusion coefficient from non-steady state chloride migration and natural chloride diffusion. The cement hydrates were investigated using X-ray diffraction and thermogravimetric analysis. The chloride binding capacity was evaluated based on the chloride adsorption from the solutions using the adsorption isotherm. The aluminate cement, as an available alumina source, can stimulate the formulation of layered double hydroxides, which in turn can increase the chloride binding capacity. The results of mercury intrusion porosimetry show that non-substituted (control) and substituted (only aluminate cement) specimens have capillary pore volume 8.9 vol % and 8.2 vol %, respectively. However, the specimen substituted with aluminate cement and calcium carbonate shows a higher capillary volume (12.9 vol %), which correlates with the chloride diffusion coefficient. Although the specimen substituted with calcium carbonate has a higher chloride binding capacity than the control, it does not necessarily affect the decrease in the chloride diffusion coefficient. The capillary pore volume can affect not only the chloride diffusion but also the chloride adsorption.
Evidence of Stress Development as a Source of Driving Force for Grain-Boundary Migration in a Ni Bicrystalline TEM Specimen
Authors: Lee Sung Bo , Jung Jinwook , Han Heung Nam
Published: January 2020

In a previous study, using high-resolution transmission electron microscopy (HRTEM), we examined grain-boundary migration behavior in a Ni bicrystal. A specimen for transmission electron microscopy (TEM) was prepared using focused ion beam. The Ni lamella in the specimen was composed of two grains with surface normal directions of [1 0 0] and [1 1 0]. As the lamella was heated to 600 °C in a TEM, it was subjected to compressive stresses. The stress state of the Ni lamella approximated to the isostress condition, which was confirmed by a finite element method. However, the stress development was not experimentally confirmed in the previous study. In the present study, we present an observation of stacking faults with a length of 40–70 nm at the grain boundary as direct evidence of the stress development.
Drug Delivery with Polymeric Nanocarriers—Cellular Uptake Mechanisms
Authors: Nelemans Levi Collin , Gurevich Leonid
Published: January 2020

Nanocarrier-based systems hold a promise to become “Dr. Ehrlich’s Magic Bullet” capable of delivering drugs, proteins and genetic materials intact to a specific location in an organism down to subcellular level. The key question, however, how a nanocarrier is internalized by cells and how its intracellular trafficking and the fate in the cell can be controlled remains yet to be answered. In this review we survey drug delivery systems based on various polymeric nanocarriers, their uptake mechanisms, as well as the experimental techniques and common pathway inhibitors applied for internalization studies. While energy-dependent endocytosis is observed as the main uptake pathway, the integrity of a drug-loaded nanocarrier upon its internalization appears to be a seldomly addressed problem that can drastically affect the uptake kinetics and toxicity of the system in vitro and in vivo.
Fucoidan Derived from Fucus vesiculosus Inhibits the Development of Human Ovarian Cancer via the Disturbance of Calcium Homeostasis, Endoplasmic Reticulum Stress, and Angiogenesis
Authors: Bae Hyocheol , Lee Jin-Young , Yang Changwon , Song Gwonhwa , Lim Whasun
Published: January 2020

Marine organisms are sources of several natural compounds with potential clinical use. However, only a few marine-based pharmaceuticals have been approved for use due to limited knowledge on their biological activities. Here, we identified the functional role of fucoidan extracted from Fucus vesiculosus on ovarian cancer. Fucoidan increased the death of ES-2 and OV-90 cells, through a reduction in proliferation, cell cycle arrest, releases of cytochrome c, reactive oxygen species (ROS) generation, and endoplasmic reticulum (ER) stress. Additionally, fucoidan increased the concentration of cytosolic and mitochondrial calcium in both cells. The decrease of cell proliferation was controlled by the inactivation of PI3K and MAPK signaling cascades in ES-2 and OV-90 cells. In a toxicity assay with normal zebrafish larvae, fucoidan did not induce toxicity, cardiotoxicity, development, kinesis, and apoptosis at different concentrations. However, it disrupted tumor formation and vascular development in a zebrafish xenograft model and angiogenesis transgenic (Tg, fli1-eGFP) model, respectively. Collectively, the results indicate that fucoidan may be a novel pharmaceutical for the management of human ovarian cancer.
Aerosolizable Marine Phycotoxins and Human Health Effects: In Vitro Support for the Biogenics Hypothesis
Authors: Van Acker Emmanuel , De Rijcke Maarten , Asselman Jana , Beck Ilse M. , Huysman Steve , Vanhaecke Lynn , De Schamphelaere Karel A.C. , Janssen Colin R.
Published: January 2020

Respiratory exposure to marine phycotoxins is of increasing concern. Inhalation of sea spray aerosols (SSAs), during harmful Karenia brevis and Ostreopsis ovata blooms induces respiratory distress among others. The biogenics hypothesis, however, suggests that regular airborne exposure to natural products is health promoting via a downregulation of the mechanistic target of rapamycin (mTOR) pathway. Until now, little scientific evidence supported this hypothesis. The current explorative in vitro study investigated both health-affecting and potential health-promoting mechanisms of airborne phycotoxin exposure, by analyzing cell viability effects via cytotoxicity assays and effects on the mTOR pathway via western blotting. To that end, A549 and BEAS-2B lung cells were exposed to increasing concentrations (ng·L−1–mg·L−1) of (1) pure phycotoxins and (2) an extract of experimental aerosolized homoyessotoxin (hYTX). The lowest cell viability effect concentrations were found for the examined yessotoxins (YTXs). Contradictory to the other phycotoxins, these YTXs only induced a partial cell viability decrease at the highest test concentrations. Growth inhibition and apoptosis, both linked to mTOR pathway activity, may explain these effects, as both YTXs were shown to downregulate this pathway. This proof-of-principle study supports the biogenics hypothesis, as specific aerosolizable marine products (e.g., YTXs) can downregulate the mTOR pathway.
Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Cancer Stem Cell-Enriched Tumourspheres
Authors: Cotas João , Marques Vanda , Afonso Marta B. , Rodrigues Cecília M. P. , Pereira Leonel
Published: January 2020

Gigartina pistillata is a red seaweed common in Figueira da Foz, Portugal. Here, the antitumour potential of G. pistillata carrageenan, with a known variable of the life cycle, the female gametophyte (FG) and tetrasporophyte (T) was evaluated against colorectal cancer stem cell (CSC) -enriched tumourspheres. FTIR-ATR analysis of G. pistillata carrageenan extracts indicated differences between life cycle phases, being FG a κ/ι hybrid carrageenan and T a ʎ/ξ hybrid. Both carrageenan extracts presented IC50 values inferior to 1 μg/mL in HT29-derived CSC-enriched tumourspheres, as well as reduced tumoursphere area. The two extracts were also effective at reducing cellular viability in SW620- and SW480-derived tumourspheres. These results indicate that carrageenans extracted from two G. pistillata life cycle phases have antitumour potential against colorectal cancer stem-like cells, specially the T carrageenan.
Structure-Based Virtual Screening, Molecular Dynamics and Binding Free Energy Calculations of Hit Candidates as ALK-5 Inhibitors
Authors: Araujo Sheila C. , Maltarollo Vinicius G. , Almeida Michell O. , Ferreira Leonardo L. G. , Andricopulo Adriano D. , Honorio Kathia M.
Published: January 2020

Activin-like kinase 5 (ALK-5) is involved in the physiopathology of several conditions, such as pancreatic carcinoma, cervical cancer and liver hepatoma. Cellular events that are landmarks of tumorigenesis, such as loss of cell polarity and acquisition of motile properties and mesenchymal phenotype, are associated to deregulated ALK-5 signaling. ALK-5 inhibitors, such as SB505154, GW6604, SD208, and LY2157299, have recently been reported to inhibit ALK-5 autophosphorylation and induce the transcription of matrix genes. Due to their ability to impair cell migration, invasion and metastasis, ALK-5 inhibitors have been explored as worthwhile hits as anticancer agents. This work reports the development of a structure-based virtual screening (SBVS) protocol aimed to prospect promising hits for further studies as novel ALK-5 inhibitors. From a lead-like subset of purchasable compounds, five molecules were identified as putative ALK-5 inhibitors. In addition, molecular dynamics and binding free energy calculations combined with pharmacokinetics and toxicity profiling demonstrated the suitability of these compounds to be further investigated as novel ALK-5 inhibitors.
Rationally Designed Ruthenium Complexes for Breast Cancer Therapy
Authors: Golbaghi Golara , Castonguay Annie
Published: January 2020

Since the discovery of the anticancer potential of ruthenium-based complexes, several species were reported as promising candidates for the treatment of breast cancer, which accounts for the greatest number of new cases in women every year worldwide. Among these ruthenium complexes, species containing bioactive ligand(s) have attracted increasing attention due to their potential multitargeting properties, leading to anticancer drug candidates with a broader range of cellular targets/modes of action. This review of the literature aims at providing an overview of the rationally designed ruthenium-based complexes that have been reported to date for which ligands were carefully selected for the treatment of hormone receptor positive breast cancers (estrogen receptor (ER+) or progesterone receptor (PR+)). In addition, this brief survey highlights some of the most successful examples of ruthenium complexes reported for the treatment of triple negative breast cancer (TNBC), a highly aggressive type of cancer, regardless of if their ligands are known to have the ability to achieve a specific biological function.
Erratum: Del Castillo, R.M., et al. Electronic Peculiarities of a Self-Assembled M12L24 Nanoball (M = Pd+2, Cr, or Mo). Molecules 2019, 24, 771
Authors: del Castillo Roxana Mitzayé , Salcedo Roberto , Martínez Ana , Ramos Estrella , Sansores Luis E.
Published: January 2020

The author wishes to make the following correction to this paper [...]
Revealing the Usefulness of Aroma Networks to Explain Wine Aroma Properties: A Case Study of Portuguese Wines
Authors: Petronilho Sílvia , Lopez Ricardo , Ferreira Vicente , Coimbra Manuel A. , Rocha Sílvia M.
Published: January 2020

Wine aroma is the result of complex interactions between volatile compounds and non-volatile ones and individual perception phenomenon. In this work, an aroma network approach, that links volatile composition (chromatographic data) with its corresponding aroma descriptors was used to explain the wine aroma properties. This concept was applied to six monovarietal wines from Bairrada Appellation (Portugal) and used as a case study. A comprehensive determination of the wines’ volatile composition was done (71 variables, i.e., volatile components), establishing a workflow that combines extraction techniques and gas chromatographic analysis. Then, a bipartite network-based approach consisting of two different nodes was built, one with 19 aroma descriptors, and the other with the corresponding volatile compound(s). To construct the aroma networks, the odor active values were calculated for each determined compound and combined with the bipartite network. Finally, the aroma network of each wine was compared with sensory descriptive analysis. The analysis of the specific aroma network of each wine revealed that Sauvignon Blanc and Arinto white wines present higher fruity (esters) and sweet notes (esters and C13 norisoprenoids) than Bical wine. Sauvignon Blanc also exhibits higher toasted aromas (thiols) while Arinto and Bical wines exhibit higher flowery (C13 norisoprenoids) and herbaceous notes (thiols), respectively. For red wines, sweet fruit aromas are the most abundant, especially for Touriga Nacional. Castelão and Touriga Nacional wines also present toasted aromas (thiols). Baga and Castelão wines also exhibit fusel/alcohol notes (alcohols). The proposed approach establishes a chemical aroma fingerprint (aroma ID) for each type of wine, which may be further used to estimate wine aroma characteristics by projection of the volatile composition on the aroma network.
Synthesis of Chromium(II) Complexes with Chelating Bis(alkoxide) Ligand and Their Reactions with Organoazides and Diazoalkanes
Authors: Kurup Sudheer S. , Staples Richard J. , Lord Richard L. , Groysman Stanislav
Published: January 2020

Synthesis of new chromium(II) complexes with chelating bis(alkoxide) ligand [OO]Ph (H2[OO]Ph = [1,1′:4′,1′’-terphenyl]-2,2′’-diylbis(diphenylmethanol)) and their subsequent reactivity in the context of catalytic production of carbodiimides from azides and isocyanides are described. Two different Cr(II) complexes are obtained, as a function of the crystallization solvent: mononuclear Cr[OO]Ph(THF)2 (in toluene/THF, THF = tetrahydrofuran) and dinuclear Cr2([OO]Ph)2 (in CH2Cl2/THF). The electronic structure and bonding in Cr[OO]Ph(THF)2 were probed by density functional theory calculations. Isolated Cr2([OO]Ph)2 undergoes facile reaction with 4-MeC6H4N3, 4-MeOC6H4N3, or 3,5-Me2C6H3N3 to yield diamagnetic Cr(VI) bis(imido) complexes; a structure of Cr[OO]Ph(N(4-MeC6H4))2 was confirmed by X-ray crystallography. The reaction of Cr2([OO]Ph)2 with bulkier azides N3R (MesN3, AdN3) forms paramagnetic products, formulated as Cr[OO]Ph(NR). The attempted formation of a Cr–alkylidene complex (using N2CPh2) instead forms chromium(VI) bis(diphenylmethylenehydrazido) complex Cr[OO]Ph(NNCPh2)2. Catalytic formation of carbodiimides was investigated for the azide/isocyanide mixtures containing various aryl azides and isocyanides. The formation of carbodiimides was found to depend on the nature of organoazide: whereas bulky mesitylazide led to the formation of carbodiimides with all isocyanides, no carbodiimide formation was observed for 3,5-dimethylphenylazide or 4-methylphenylazide. Treatment of Cr2([OO]Ph)2 or H2[OO]Ph with NO+ leads to the formation of [1,2-b]-dihydroindenofluorene, likely obtained via carbocation-mediated cyclization of the ligand.
A Novel Dual Fluorochrome Near-Infrared Imaging Probe for Potential Alzheimer’s Enzyme Biomarkers-BACE1 and Cathepsin D
Authors: Tam Jenny M. , Josephson Lee , Pilozzi Alexander R. , Huang Xudong
Published: January 2020

A molecular imaging probe to fluorescently image the β-site of the amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) and cathepsin D (CatD) enzymes associated with Alzheimer’s disease (AD) was designed and synthesized. This imaging probe was built upon iron oxide nanoparticles (cross-linked dextran iron oxide nanoparticles, or CLIO). Peptide substrates containing a terminal near-infrared fluorochrome (fluorophore emitting at 775 nm for CatD or fluorophore emitting at 669 nm for BACE1) were conjugated to the CLIO nanoparticles. The CatD substrate contained a phenylalanine-phenylalanine cleavage site more specific to CatD than BACE1. The BACE1 substrate contained the sequence surrounding the leucine-asparagine cleavage site of the BACE1 found in the Swedish mutation of APP, which is more specific to BACE1 than CatD. These fluorescently-labeled peptide substrates were then conjugated to the nanoparticle. The nanoparticle probes were purified by gel filtration, and their fluorescence intensities were determined using a fluorescence plate reader. The CatD peptide substrate demonstrated a 15.5-fold increase in fluorescence when incubated with purified CatD enzyme, and the BACE1 substrate exhibited a 31.5-fold increase in fluorescence when incubated with purified BACE1 enzyme. Probe specificity was also demonstrated in the human H4 neuroglioma cells and the H4 cells stably transfected with BACE1 in which the probe monitored enzymatic cleavage. In the H4 and H4-BACE1 cells, BACE1 and active CatD activity increased, an occurrence that was reflected in enzyme expression levels as determined by immunoblotting. These results demonstrate the applicability of this probe for detecting potential Alzheimer’s enzyme biomarkers.
Quality Control of Psoralea corylifolia L. Based on High-Speed Countercurrent Chromatographic Fingerprinting
Authors: Wu Xiaoxue , Gao Xuemin , Liu Xinmei , Zhang Shuyi , Yang Huayu , Zhu Xuan , Song Hua , Li Funan , Chen Qing
Published: January 2020

Traditional Chinese medicine (TCM)has played an important role in promoting the health of Chinese people. The TCM Psoralea corylifolia L. has been used in the treatment of various kinds of diseases including enuresis, vitiligo, and calvities. However, therapeutic effects of P. corylifolia L. have often influenced by the quality of plants. So, it is very important to control the quality of P. corylifolia L. In this study, analytical high-speed countercurrent chromatography (HSCCC) was successfully used to fingerprint P. corylifolia L. Samples of P. corylifolia L. were extracted by ultrasonic extraction. n-hexane-ethyl acetate–methanol–water at a ratio of 5:5.5:6.5:5 (v/v) was selected as a two-phase solvent system and the condition of HSCCC were optimized in order to good separation. And the method of HSCCC was verified (reproducibility, precision, and stability). HSCCC chromatograms exhibited six common peaks, which were selected as indicator compounds for the quality control of P. corylifolia L. Within 20 types of medicinal materials, chemical components are similar, but the levels of components are quite different in HSCCC fingerprint. The present results demonstrate that the HSCCC method provides a reliable basis for the quality control of P. corylifolia L. and can also be applied to confirm the authenticity of plant materials.
Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from Crotalus durissus terrificus by Hispidulin from Moquiniastrum floribundum
Authors: dos Santos Junior Adeilso Bispo , Tamayose Cinthia I. , Ferreira Marcelo J. P. , Belchor Mariana N. , Costa Caroline R. C. , de Oliveira Marcos Antonio , Toyama Marcos Hikari
Published: January 2020

Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. However, some proteins, such as snake secretory phospholipase A2 (sPLA2), have never been investigated using this approach. The purpose of this study was to evaluate the use of a new method for screening natural compounds using a bioaffinity-guided ultrafiltration method on Crotalus durissus terrificus sPLA2 followed by HPLC-MS to identify the compounds, and this method could be used to discover new anti-inflammatory compounds from the various organisms originating from biodiversity. Different extracts were selected to evaluate their ability to inhibit sPLA2 activity. The extracts were incubated with sPLA2 and the resulting mixture was ultrafiltrated to elute unbound components. The resulting compounds were identified by HPLC-MS. We identified hispidulin as one of the components present in the Moquiniastrum floribundum leaf and evaluated the ability of this isolated compound to neutralize the inflammatory activity of sPLA2 from Crotalus durissus terrificus.
Synthesis and Thermal Investigations of Eleven-Membered Ring Systems Containing One of the Heavier Group 14 Element Atoms Si, Ge, and Sn
Authors: Eleya Nadi , Appiah Clement , Lork Enno , Gogolin Mathias , Gesing Thorsten M. , Stauch Tim , Staubitz Anne
Published: January 2020

Unique eleven-membered rings containing silicon, germanium, and tin were synthesized in good yields by the reactions of the corresponding 1,2-bis((2-bromothiophen-3-yl)methoxy)benzenes with (C6H5)2ECl2 where E = Sn, Ge, Si. The Sn and Ge congeners were crystallized, but the conformers that these rings crystallized in, were quite different. As confirmed by Density Functional Theory (DFT) calculations, (C28H22O2S2Sn) assumes a unique crystal structure that leaves more room around the tetrel atom as compared to the crystal structure of the corresponding Ge compound. In the latter, the central cavity is quite open, whereas in the former, one of the methylene groups can fold inwards. Another consequence is the influence on the planes of the aromatic rings flanking the heterocycle. In the Ge case, the benzene ring is folded away from the central cavity, whereas in the Sn case, it is almost parallel to the imaginary axis through the center of the ring. Thermal analysis investigations (TGA and DSC methods) of these eleven-membered rings suggested the loss of a phenyl group in the first decomposition step. The decomposition temperature decreased from the Si containing heterocycle to Ge and was lowest for the Sn containing heterocycle.
HMPV in Immunocompromised Patients: Frequency and Severity in Pediatric Oncology Patients
Authors: Martinez-Rodriguez Cesar , Banos-Lara Ma. del Rocio
Published: January 2020

Cancer is the first cause of death by disease in childhood globally. The most frequent types of cancers in children and adolescents are leukemias, followed by brain and central nervous system tumors and lymphomas. The recovery rate of cancer in children is around 80% in developed countries and up to 30% in developing countries. Some of the main causes of complications in children and adolescents with cancer are respiratory viral infections, mainly in bone marrow-transplanted patients. Respiratory viruses have been detected in the bronchoalveolar lavage or nasal wash specimens from cancer patients with or without respiratory illness symptoms. Human metapneumovirus (HMPV) is within the ten most common viruses that are encountered in samples from pediatric patients with underlying oncology conditions. In most of cases, HMPV is found as the only viral agent, but co-infection with other viruses or with bacterial agents has also been reported. The discrepancies between the most prevalent viral agents may be due to the different populations studied or the range of viral agents tested. Some of the cases of infection with HMPV in cancer patients have been fatal, especially in those who have received a hematopoietic stem cell transplant. This review seeks to show a general view of the participation of HMPV in respiratory illness as a complication of cancer in childhood and adolescence.
Seasonality and Small Spatial-Scale Variation of Chlorophyll a Fluorescence in Bryophyte Syntrichia ruralis [Hedw.] in Semi-Arid Sandy Grassland, Hungary
Authors: Ruchika  , Csintalan Zsolt , Péli Evelin Ramóna
Published: January 2020

Bryophytes face challenges due to global climate change which is leading to in-depth research in monitoring and studying their photosynthetic activity. The aim of this preliminary experiment was to study the seasonal variation trend in the chlorophyll a fluorescence parameters, Fv/Fm (ratio of variable to maximum fluorescence), photochemical fluorescence quenching (qP), photochemical quantum yield of photosystem II (ΦPS II), fluorescence quenching (qN), and non-photochemical quenching (NPQ), in the moss cushions of Syntrichia ruralis [Hedw.] collected from semi-arid sandy dunes for two slopes i.e., north-east (NE) and south-west (SW) direction. Our results showed a seasonal and small-spatial scale variation trend in all chlorophyll fluorescence parameters. These variations are due to different seasonal conditions referring to different degrees of environmental stress. ΦPS II and qP values were maximum in winter and in spring seasons while Fv/Fm, NPQ and qN were maximum in summer. Based on the different exposition of dunes, the SW slope showed increased values of the effective quantum yield of PS II and qP in comparison to the NE slope due to the optimal microclimate conditions for their expansion. These results may refer to the future changing in diversification and coverage of the Syntrichia species in semi-arid sandy grassland due to more effective metabolism in the beneficial microclimatic conditions.
Targeting Specific Sites in Biological Systems with Synchrotron X-Ray Microbeams for Radiobiological Studies at the Photon Factory
Authors: Yokoya Akinari , Usami Noriko
Published: January 2020

X-ray microbeams have been used to explore radiobiological effects induced by targeting a specific site in living systems. Synchrotron radiation from the Photon Factory, Japan, with high brilliance and highly parallel directionality is a source suitable for delivering a particular beam size or shape, which can be changed according to target morphology by using a simple metal slit system (beam size from 5 μm to several millimeters). Studies have examined the non-targeted effects, called bystander cellular responses, which are thought to be fundamental mechanisms of low-dose or low-dose-rate effects in practical radiation risk research. Narrow microbeams several tens of micrometers or less in their size targeted both the cell nucleus and the cytoplasm. Our method combined with live-cell imaging techniques has challenged the traditional radiobiological dogma that DNA damage is the only major cause of radiation-induced genetic alterations and is gradually revealing the role of organelles, such as mitochondria, in these biological effects. Furthermore, three-dimensionally cultured cell systems have been used as microbeam targets to mimic organs. Combining the spatial fractionation of X-ray microbeams and a unique ex vivo testes organ culture technique revealed that the tissue-sparing effect was induced in response to the non-uniform radiation fields. Spatially fractionated X-ray beams may be a promising tool in clinical radiation therapy.
An Enhanced Detection System of Drill Rod Bending Degree Based on Two-Dimensional Laser
Authors: Wu Lingling , Wen Guojun , Wang Yudan , Xu Xinjian
Published: January 2020

Drill rod straightness has to be strictly controlled and the maximum bending degree detection needs to be used in the straightening process. The mechanical bending degree measurement depends on machinery instruments and workers’ experience, often with low efficiency and precision. While the optical inspection, as a non-contact detection method, with higher precision and lower installation accuracy requirements, is frequently applied in the online detection system. Based on this, an enhanced bending degree detection system for a drill rod is proposed in this paper. Compared to the existing detection system, the main progress is to use a two-dimensional laser to quickly obtain arc profile data and fit with ellipse. Segment inspection idea is also utilized is this system as the camera that could obtain the whole drill rod in one shot needs extremely high resolution and price. A specialized algorithm is designed to fit the cross-section shape and whole centerline displacement based on the least square method. Some laboratory tests are conducted to verify this detection system, findings of which are compared to manual measured results. The maximum bending degree error is 2.14 mm and the maximum position error is 8.21 mm, which are both within the tolerance of error. Those results show the feasibility and precision of this enhanced detection system.
Joint Time-Reversal Space-Time Block Coding and Adaptive Equalization for Filtered Multitone Underwater Acoustic Communications
Authors: Sun Lin , Yan Ming , Li Haisen , Xu Yanjie
Published: January 2020

Underwater acoustic (UWA) sensor networks demand high-rate communications with high reliability between sensor nodes for massive data transmission. Filtered multitone (FMT) is an attractive multicarrier technique used in high-rate UWA communications, and can obviously shorten the span of intersymbol interference (ISI) with high spectral efficiency and low frequency offset sensitivity by dividing the communication band into several separated wide sub-bands without guard bands. The joint receive diversity and adaptive equalization scheme is often used as a general ISI suppression technique in FMT-UWA communications, but large receive array for high diversity gain has an adverse effect on the miniaturization of UWA sensor nodes. A time-reversal space-time block coding (TR-STBC) technique specially designed for frequency-selective fading channels can replace receive diversity with transmit diversity for high diversity gain, and therefore is helpful for ISI suppression with simple receive configuration. Moreover, the spatio-temporal matched filtering (MF) in TR-STBC decoding can mitigate ISI obviously, and therefore is of benefit to lessen the complexion of adaptive equalization for post-processing. In this paper, joint TR-STBC and adaptive equalization FMT-UWA communication method is proposed based on the merit of TR-STBC. The proposed method is analyzed in theory, and its performance is assessed using simulation analysis and real experimental data collected from an indoor pool communication trial. The validity of the proposed method is proved through comparing the proposed method with the joint single-input–single-output (SISO) and adaptive equalization method and the joint single-input–multiple-output (SIMO) and adaptive equalization method. The results show that the proposed method can achieve better communication performance than the joint SISO and adaptive equalization method, and can achieve similar performance with more simpler receive configuration as the joint SIMO and adaptive equalization method.
A Novel Evidence Conflict Measurement for Multi-Sensor Data Fusion Based on the Evidence Distance and Evidence Angle
Authors: Deng Zhan , Wang Jianyu
Published: January 2020

As an important method for uncertainty modeling, Dempster–Shafer (DS) evidence theory has been widely used in practical applications. However, the results turned out to be almost counter-intuitive when fusing the different sources of highly conflicting evidence with Dempster’s combination rule. In previous researches, most of them were mainly dependent on the conflict measurement method between the evidence represented by the evidence distance. However, it is inaccurate to characterize the evidence conflict only through the evidence distance. To address this issue, we comprehensively consider the impacts of the evidence distance and evidence angle on conflicts in this paper, and propose a new method based on the mutual support degree between the evidence to characterize the evidence conflict. First, the Hellinger distance measurement method is proposed to measure the distance between the evidence, and the sine value of the Pignistic vector angle is used to characterize the angle between the evidence. The evidence distance indicates the dissimilarity between the evidence, and the evidence angle represents the inconsistency between the evidence. Next, two methods are combined to get a new method for measuring the mutual support degree between the evidence. Afterward, the weight of each evidence is determined by using the mutual support degree between the evidence. Then, the weights of each evidence are utilized to modify the original evidence to achieve the weighted average evidence. Finally, Dempster’s combination rule is used for fusion. Some numerical examples are given to illustrate the effectiveness and reasonability for the proposed method.