bluuebunny/crossref_metadata_2025 huggingface dataset:
Monitoring of Natural Pigments in Henna and Jagua Tattoos for Fake Detection
Authors: Rubio Laura , Lores Marta , Garcia-Jares Carmen
Published: September 2020

Temporary tattoos are a popular alternative to permanent ones. Some of them use natural pigments such as lawsone in the famous henna tattoos. Recently, jagua tattoos, whose main ingredients are genipin and geniposide, have emerged as an interesting option. This study was conducted to identify the presence and concentration of henna and jagua active ingredients (lawsone; genipin and geniposide, respectively) in commercial tattoo samples. Since natural pigments are often mixed with additives such as p-phenylenediamine (PPD) in the case of henna, PPD has been included in the study. Green and simple extraction methods based on vortex or ultrasound-assisted techniques have been tested. To determine the compounds of interest liquid chromatography (LC) with diode-array detection (DAD) has been applied; and PPD absence was confirmed by LC-QTOF (quadrupole-time of flight tandem mass spectrometry). This work demonstrated that only one out of 14 henna samples analyzed contained lawsone. For jaguas, genipin was found in all samples, while geniposide only in two. Therefore, quality control analysis on these semi-permanent tattoos is considered necessary to detect these ingredients in commercial mixtures, as well as to uncover possible fraud in products sold as natural henna.
Volatile Compounds Emitted from the Cat Urine Contaminated Carpet before and after Treatment with Marketed Cleaning Products: A Simultaneous Chemical and Sensory Analysis
Authors: Banik Chumki , Koziel Jacek A. , Flickinger Elizabeth
Published: September 2020

Urination on carpet and subflooring can develop into a persistent and challenging problem when trying to mitigate odor. Very little or no information is published on how volatile organic compounds (VOCs) change over time when urine is deposited on a carpet covering a plywood-type subflooring. This research has investigated the VOCs emitted from carpet + subflooring (control), carpet + subflooring sprayed with water (control with moisture), and cat urine-contaminated carpet + subflooring (treatment) over time (day 0 and 15). In addition, the study has recorded the effect of four popular cleaning product applications on VOCs emitted from carpet and evaluated their efficacy in eliminating cat urine related indoor odors over time (days 0 and 15). Carpet-subflooring with all treatments were also contaminated with Micrococcus luteus, a nonmotile obligate aerobe commonly found in household dust, to observe the impact of the aerobe on carpet-subflooring VOCs emission. VOCs emitted from carpet + subflooring receiving different treatments were collected from headspace using solid-phase microextraction (SPME). The VOCs were analyzed using a gas chromatography-mass spectrometry olfactometry (GC-MS-O). Many common VOCs were released from the carpet on day 0 and day 15, specifically from urine contamination. Cleaning products were effective in masking several potent odors of cat urine contaminated carpet VOCs on day 0 but were unable to remove the odor that appeared on day 15 in most cases.
Thermal and Multispectral Remote Sensing for the Detection and Analysis of Archaeologically Induced Crop Stress at a UK Site
Authors: James Katherine , Nichol Caroline J. , Wade Tom , Cowley Dave , Gibson Poole Simon , Gray Andrew , Gillespie Jack
Published: September 2020

In intensively cultivated landscapes, many archaeological remains are buried under the ploughed soil, and detection depends on crop proxies that express subsurface features. Traditionally these proxies have been documented in visible light as contrasting areas of crop development commonly known as cropmarks. However, it is recognised that reliance on the visible electromagnetic spectrum has inherent limitations on what can be documented, and multispectral and thermal sensors offer the potential to greatly improve our ability to detect buried archaeological features in agricultural fields. The need for this is pressing, as ongoing agricultural practices place many subsurface archaeological features increasingly under threat of destruction. The effective deployment of multispectral and thermal sensors, however, requires a better understanding of when they may be most effective in documenting archaeologically induced responses. This paper presents the first known use of the FLIR Vue Pro-R thermal imager and Red Edge-M for exploring crop response to archaeological features from two UAV surveys flown in May and June 2019 over a known archaeological site. These surveys provided multispectral imagery, which was used to create vegetation index (VI) maps, and thermal maps to assess their effectiveness in detecting crop responses in the temperate Scottish climate. These were visually and statistically analysed using a Mann Whitney test to compare temperature and reflectance values. While the study was compromised by unusually damp conditions which reduced the potential for cropmarking, the VIs (e.g., Normalised Difference Vegetation Index, NDVI) did show potential to detect general crop stress across the study site when they were statistically analysed. This demonstrates the need for further research using multitemporal data collection across case study sites to better understand the interactions of crop responses and sensors, and so define appropriate conditions for large-area data collection. Such a case study-led multitemporal survey approach is an ideal application for UAV-based documentation, especially when “perfect” conditions cannot be guaranteed.
Bud-Derivatives, a Novel Source of Polyphenols and How Different Extraction Processes Affect Their Composition
Authors: Turrini Federica , Donno Dario , Beccaro Gabriele Loris , Pittaluga Anna , Grilli Massimo , Zunin Paola , Boggia Raffaella
Published: September 2020

The use of herbal food supplements, as a concentrate form of vegetable extracts, increased so much over the past years to count them among the relevant sources of dietetic polyphenols. Bud-derivatives are a category of botanicals perceived as a “new entry” in this sector since they are still poorly studied. Due to the lack of a manufacturing process specification, very different products can be found on the market in terms of their polyphenolic profile depending on the experimental conditions of manufacturing. In this research two different manufacturing processes, using two different protocols, and eight species (Carpinus betulus L., Cornus mas L., Ficus carica L., Fraxinus excelsior L., Larix decidua Mill., Pinus montana Mill., Quercus petraea (Matt.) Liebl., Tilia tomentosa Moench), commonly used to produce bud-derivatives, have been considered as a case study. An untargeted spectroscopic fingerprint of the extracts, coupled to chemometrics, provide to be a useful tool to identify these botanicals. The targeted phytochemical fingerprint by HPLC provided a screening of the main bud-derivatives polyphenolic classes highlighting a high variability depending on both method and protocol used. Nevertheless, ultrasonic extraction proved to be less sensitive to the different extraction protocols than conventional maceration regarding the extract polyphenolic profile.
Phytochemical Components and Bioactivity Assessment among Twelve Strawberry (Arbutus unedo L.) Genotypes Growing in Morocco Using Chemometrics
Authors: Zitouni Hafida , Hssaini Lahcen , Messaoudi Zerhoune , Ourradi Hamza , Viuda-Martos Manuel , Hernández Francisca , Ercisli Sezai , Hanine Hafida
Published: September 2020

There are not many exhaustive works emphasizing the amount of genetic diversity among the strawberry tree (Arbutus unedo L.) genotypes in Morocco. This work aims to assess the biochemical composition of strawberry tree fruits, as well as to establish the variation of this composition among them. In this study, total phenols (TP), total flavonoids (TF), condensed tannins (CT) and hydrolyzable tannins (HT), total anthocyanins (TA), and free radical scavenging activity through ABTS were investigated in strawberry tree fruits. Furthermore, qualitative and quantitative analyses of individual phenolic compounds by high-performance liquid chromatography (HPLC) were carried out. Color parameters such as lightness (L*), Chroma (c*), and hue angle (h°) were also investigated. All studied variables showed highly significant differences among all samples with the exception of hydrolyzable tannins and chromatic coordinates. TP varied from 22.63 ± 1.74 to 39.06 ± 2.44 mg GAE/g DW, TF varied from 3.30 ± 0.60 to 8.62 ± 1.10 mg RE/g DW, and TA ranged between 0.12 ± 0.06 and 0.66 ± 0.15 mg cya-3-glu/100 g DW. In addition, CT and HT amounts were in the range of 10.41 ± 1.07–16.08 ± 1.50 mg TAE/g DW and 4.08 ± 2.43–6.34 ± 3.47 TAE/g DW, respectively. Moreover, the IC50 value (ABTS) ranged between 1.75 and 19.58 mg AAE/g DW. 17 phenolic compounds were detected in strawberry tree fruits. Gallocatechol and catechin were the most abundant phenolic compounds. Matrix of correlations revealed significant positive and negative correlations among variables particularly c*, a*, and b*. Principal component analysis (PCA) showed that the first three components formed than 68% of the total inertia. The following variables gallic acid, protocatechuic, gallocatechin, gallic acid derivative, chlorogenic acid, syringic acid, ellagic acid derivative II, L*, and h* were the most involved in the total variance explained. Hierarchical clustering classified samples into one main cluster, with a single branch. The results highlight a high biochemical diversity within studied strawberry genotypes, which is probably more genetically related.
Conversion of Exhausted Sugar Beet Pulp into Fermentable Sugars from a Biorefinery Approach
Authors: Marzo Cristina , Díaz Ana Belén , Caro Ildefonso , Blandino Ana
Published: September 2020

In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as a generic microbial culture medium, was carried out by using exhausted sugar beet pulp pellets (ESBPPs) as raw material. For this purpose, the hydrolysis was performed through the direct addition of the fermented ESBPPs obtained by fungal solid-state fermentation (SSF) as an enzyme source. By directly using this fermented solid, the stages for enzyme extraction and purification were avoided. The effects of temperature, fermented to fresh solid ratio, supplementation of fermented ESBPP with commercial cellulase, and the use of high-solid fed-batch enzymatic hydrolysis were studied to obtain the maximum reducing sugar (RS) concentration and productivity. The highest RS concentration and productivity, 127.3 g·L−1 and 24.3 g·L−1·h−1 respectively, were obtained at 50 °C and with an initial supplementation of 2.17 U of Celluclast® per gram of dried solid in fed-batch mode. This process was carried out with a liquid to solid ratio of 4.3 mL·g−1 solid, by adding 15 g of fermented solid and 13.75 g of fresh solid at the beginning of the hydrolysis, and then the same amount of fresh solid 3 times every 2.5 h. By this procedure, ESBPP can be used to produce a generic microbial feedstock, which contains a high concentration of monosaccharides.
Study on the Spatial Differentiation of Public Health Service Capabilities of European Union under the Background of the COVID-19 Crisis
Authors: Ding Xuhui , Cai Zhongyao , Zhu Wei , Fu Zhu
Published: September 2020

Access to public health services is a cause that benefits the people and concerns the vital interests of the people. Everyone has access to basic health care services. The continuous improvement in people’s health is an important indicator of the improvement in people’s quality of life. This paper selects data from the European Union (EU) on aspects of public health expenditure, medical care resources, and government emergency coordination capacity from the period 2008 to 2017. Principal component analysis and factor analysis are used to measure their public health service capacity scores and conduct a comparative analysis. On this basis, the TOBIT model is adopted to explore the driving factors that lead to the spatial differentiation of public health service capabilities, and to combine it with the data of the COVID-19 epidemic as of 8 August 2020 from the official announcements of the World Health Organization and governments for further thinking. The results indicate that the public health service capacity of countries in the EU is showing a gradual increase. The capacity in Western Europe is, in turn, higher than that of Northern Europe, Southern Europe and Eastern Europe. In addition, the overall capacity in Western Europe is relatively high, but it is not balanced and stable, while Northern Europe has remained stable and balanced at a high level. Population density, degree of opening up, education level, economic development level, technological innovation level, and degree of aging have a positive effect on public health service capabilities. The level of urbanization has a negative effect on it. However, in countries with strong public health service capabilities, the epidemic of COVID-19 is more severe. The emergence of this paradox may be related to the detection capabilities of countries, the high probability of spreading thCOVID-19 epidemic, the inefficient implementation of government policy, the integrated system of the EU and the adverse selection of youth. This paper aims to improve the ability of the EU to respond to public health emergencies, improve the utilization of medical and health resources, and better protect people’s health from the perspective of public health service capacity.
High-Temperature Profile Monitoring in Gas Turbine Exhaust-Gas Diffusors with Six-Point Fiber-Optic Sensor Array
Authors: Dutz Franz J. , Boje Sven , Orth Ulrich , Koch Alexander W. , Roths Johannes
Published: September 2020

In this paper, the deployment of a newly developed, multipoint, fiber-optic temperature-sensor system for temperature distribution measurements in a 6 MW gas turbine is demonstrated. The optical sensor fiber was integrated in a stainless steel protection cable with a 1.6 mm outside diameter. It included six measurement points, distributed over a length of 110 mm. The sensor cable was mounted in a temperature probe and was positioned radially in the exhaust-gas diffusor of the turbine. With this temperature probe, the radial temperature profiles in the exhaust-gas diffusor were measured with high spatial and temporal resolution. During a test run of the turbine, characteristic temperature gradients were observed when the machine operated at different loads.
New Order-Revealing Encryption with Shorter Ciphertexts
Authors: Kim Kee Sung
Published: September 2020

As data outsourcing services have been becoming common recently, developing skills to search over encrypted data has received a lot of attention. Order-revealing encryption (OREnc) enables performing a range of queries on encrypted data through a publicly computable function that outputs the ordering information of the underlying plaintexts. In 2016, Lewi et al. proposed an OREnc scheme that is more secure than the existing practical (stateless and non-interactive) schemes by constructing an ideally-secure OREnc scheme for small domains and a “domain-extension” scheme for obtaining the final OREnc scheme for large domains. They encoded a large message into small message blocks of equal size to apply them to their small-domain scheme, thus their resulting OREnc scheme reveals the index of the first differing message block. In this work, we introduce a new ideally-secure OREnc scheme for small domains with shorter ciphertexts. We also present an alternative message-block encoding technique. Combining the proposed constructions with the domain-extension scheme of Lewi et al., we can obtain a new large-domain OREnc scheme with shorter ciphertexts or with different leakage information, but longer ciphertexts.
New Concepts in the Development and Malformation of the Arterial Valves
Authors: Henderson Deborah J. , Eley Lorraine , Chaudhry Bill
Published: September 2020

Although in many ways the arterial and atrioventricular valves are similar, both being derived for the most part from endocardial cushions, we now know that the arterial valves and their surrounding structures are uniquely dependent on progenitors from both the second heart field (SHF) and neural crest cells (NCC). Here, we will review aspects of arterial valve development, highlighting how our appreciation of NCC and the discovery of the SHF have altered our developmental models. We will highlight areas of research that have been particularly instructive for understanding how the leaflets form and remodel, as well as those with limited or conflicting results. With this background, we will explore how this developmental knowledge can help us to understand human valve malformations, particularly those of the bicuspid aortic valve (BAV). Controversies and the current state of valve genomics will be indicated.
Model of Determining the Optimal, Green Transport Route among Alternatives: Data Envelopment Analysis Settings
Authors: Vukić Luka , Poletan Jugović Tanja , Guidi Giambattista , Oblak Renato
Published: September 2020

Data envelopment analysis (DEA) is a useful method for determining relative efficiency in many types of businesses, including the transport sector. In line with the European Union’s (EU) policy of sustainable development of transport, external costs become the competitiveness factor of the transport route valorization. Presenting specific DEA settings, the paper aims to show and test a developed model for determining the optimal transport route among alternatives towards the same destination where external cost as a socio-ecological factor is included in DEA, along with transport cost (quantitative factor) and transport time (qualitative factor). In order to adhere to the principles of the least possible energy consumption, the given distance that also included in DEA settings represents the shortest route between the starting point and destination, as a unique and constant output variable. Therefore, the optimal direction selected by the DEA stands for the green route. The capabilities of the DEA, set up in this way within the broader model, are demonstrated in the practical case.
Characterization of Model Uncertainty for the Vertical Pullout Capacity of Helical Anchors in Cohesive Soils
Authors: Cheng Po , Yi Jiang Tao , Liu Fei , Dong Jun Jie
Published: September 2020

This paper conducts coupled Eulerian–Lagrangian (CEL) analysis to characterize the model uncertainty of using the cylindrical shear method (CSM) to predict the pullout capacity of helical anchors in cohesive soils. The model factor M is adopted to represent the model uncertainty, which is equal to the value of measured capacity divided by estimated solution. The model factor Mcel can be considered to be a random variable with a lognormal distribution, and its mean value and coefficient of variation (COV) are 1.02 and 0.1, respectively. Correction factor η is introduced when comparing CSM and CEL, which is found to be influenced by input parameters. The dependence on input parameters is removed by performing regression analysis and the regression equation f is obtained. Substituting the regression equation f into the original CSM constitutes the modified CSM (MCSM), and the model factor of MCSM can be modeled as a random variable with a lognormal distribution, and its mean value and COV are 1.02 and 0.13, respectively. Finally, 13 filed tests are collected to compare the prediction accuracy, the results show that the prediction error range of MCSM is mostly within 15%. The present findings might be helpful for engineers and designers to estimate the pullout capacity of helical anchors in cohesive soils more confidently.
Predicted Maps for Soil Organic Matter Evaluation: The Case of Abruzzo Region (Italy)
Authors: Piccini Chiara , Francaviglia Rosa , Marchetti Alessandro
Published: September 2020

Organic matter, an important component of healthy soils, may be used as an indicator in sustainability assessments. Managing soil carbon storage can foster agricultural productivity and environmental quality, reducing the severity and costs of natural phenomena. Thus, accurately estimating the spatial variability of soil organic matter (SOM) is crucial for sustainable soil management when planning agro-environmental measures at the regional level. SOM variability is very large in Italy, and soil organic carbon (SOC) surveys considering such variability are difficult and onerous. The study concerns the Abruzzo Region (about 10,800 km2), in Central Italy, where data from 1753 soil profiles were available, together with a Digital Elevation Model (DEM) and Landsat images. Some morphometric parameters and spectral indices with a significant degree of correlation with measured data were used as predictors for regression-kriging (RK) application. Estimated map of SOC stocks, and of SOM related to USDA (United States Department of Agriculture) texture—an additional indicator of soil quality—were produced with a satisfactory level of accuracy. Results showed that SOC stocks and SOM concentrations in relation to texture were lower in the hilly area along the shoreline, pointing out the need to improve soil management to guarantee agricultural land sustainability.
Surface Roughness Influence on Néel-, Crosstie, and Bloch-Type Charged Zigzag Magnetic Domain Walls in Nanostructured Fe Films
Authors: Favieres Cristina , Vergara José , Madurga Vicente
Published: September 2020

Charged magnetic domain walls have been visualized in soft magnetic nanostructured Fe thin films under both static and dynamic conditions. A transition in the core of these zigzagged magnetic walls from Néel-type to Bloch-type through the formation of crosstie walls has been observed. This transition in charged zigzagged walls was not previously shown experimentally in Fe thin films. For film thicknesses t < 30 nm, Néel-type cores are present, while at t ≈ 33 nm, walls with crosstie cores are observed. At t > 60 nm, Bloch-type cores are observed. Along with the visualization of these critical parameters, the dependence on the film thickness of the characteristic angle and length of the segments of the zigzagged walls has been observed and analyzed. After measuring the bistable magneto-optical behavior, the values of the wall nucleation magnetic field and the surface roughness of the films, an energetic fit to these nucleation values is presented.
Stabilization of a Clayey Soil with Ladle Metallurgy Furnace Slag Fines
Authors: Brand Alexander S. , Singhvi Punit , Fanijo Ebenezer O. , Tutumluer Erol
Published: September 2020

The research study described in this paper investigated the potential to use steel furnace slag (SFS) as a stabilizing additive for clayey soils. Even though SFS has limited applications in civil engineering infrastructure due to the formation of deleterious expansion in the presence of water, the free CaO and free MgO contents allow for the SFS to be a potentially suitable candidate for clayey soil stabilization and improvement. In this investigation, a kaolinite clay was stabilized with 10% and 15% ladle metallurgy furnace (LMF) slag fines by weight. This experimental study also included testing of the SFS mixtures with the activator calcium chloride (CaCl2), which was hypothesized to accelerate the hydration of the dicalcium silicate phase in the SFS, but the results show that the addition of CaCl2 was not found to be effective. Relative to the unmodified clay, the unconfined compressive strength increased by 67% and 91% when 10% and 15% LMF slag were utilized, respectively. Likewise, the dynamic modulus increased by 212% and 221% by adding 10% and 15% LMF slag, respectively. Specifically, the LMF slag fines are posited to primarily contribute to a mechanical rather than chemical stabilization mechanism. Overall, these findings suggest the effective utilization of SFS as a soil stabilization admixture to overcome problems associated with dispersive soils, but further research is required.
Experimental and Numerical Study on Withdrawal Strength of Different Types of Auxetic Dowels for Furniture Joints
Authors: Kasal Ali , Kuşkun Tolga , Smardzewski Jerzy
Published: September 2020

Studies on the application of auxetic metamaterials and structures in furniture joints are very limited. However, they have huge potential for use in ready-to-assemble furniture. This study aimed to design and produce different types of auxetic dowels in 3D printing technology, and experimentally and numerically analyze the withdrawal strength of these dowels. In the scope of the study, 24 auxetic dowels with different types and size of inclusions, different diameter of holes, and a non-auxetic reference dowel were designed and produced with appropriate muffs. Dowels were 3D printed from polyamide (PA12). Poisson’s ratios, withdrawal strength, contact pressures, and friction coefficients of dowels were determined theoretically by means of numerical analyses and real static compression tests. After the pre-production of dowels, the dowels with triangular inclusions have not been found to have sufficient strength and stiffness. Withdrawal strength of dowels decreased as the size of inclusions is decreased, or dowel hole diameter is increased. Furthermore, contact pressures and stresses in auxetic dowels were considerably lower than non-auxetic dowels under the withdrawal force.
Influence of Lowered Temperature on Efficiency of Concrete Repair with Polymer-Cement Repair Mortars
Authors: Wojnowski Damian , Francke Barbara , Garbacz Andrzej
Published: September 2020

This paper presents research dealing with the evaluation of the efficiency of concrete repairs with polymer-cement mortars made at low temperatures with two types of cement and modified by copolymer acrylic-styrene. The low temperature used for the tests, of about 8 °C, is representative for Central Europe, and was established based on the analysis of mean temperatures in Poland during the last 45 years. A comparative analysis of the basic properties of the mortar tested, important from the point of view of repair efficiency, was performed, i.e., flexural and compressive strength, modulus of elasticity, adhesion to the substrate, and porosity for mortars applied and cured at 8 °C and 21°, respectively. The studies were conducted using standard methods and supported with an assessment of microscopic images (1000× magnification). It was shown that when the temperature of polymer-cement composite (PCC) mortar application is lowered to values slightly exceeding the minimum film-forming temperature (MFT) temperature of the polymer modifier, the type of cement determines the effectiveness of the repair. Only for PCC mortar with CEM I sulfate-resistant types of cement was it possible to achieve the same strength parameters as at 21 °C, during 28 days of mortar curing, and at a lowered temperature. Starting from day seven of setting at both above-mentioned temperatures, a relation between the values of the flexural and compressive strength expressed as a quotient of these values, amounting to ca. 0.14–0.19, was found.
Pareto Explorer for Finding the Knee for Many Objective Optimization Problems
Authors: Cuate Oliver , Schütze Oliver
Published: September 2020

Optimization problems where several objectives have to be considered concurrently arise in many applications. Since decision-making processes are getting more and more complex, there is a recent trend to consider more and more objectives in such problems, known as many objective optimization problems (MaOPs). For such problems, it is not possible any more to compute finite size approximations that suitably represent the entire solution set. If no users preferences are at hand, so-called knee points are promising candidates since they represent at least locally the best trade-off solutions among the considered objective values. In this paper, we extend the global/local exploration tool Pareto Explorer (PE) for the detection of such solutions. More precisely, starting from an initial solution, the goal of the modified PE is to compute a path of evenly spread solutions from this point along the Pareto front leading to a knee of the MaOP. The knee solution, as well as all other points from this path, are of potential interest for the underlying decision-making process. The benefit of the approach is demonstrated in several examples.
Emotional–Behavioral Disorders in Healthy Siblings of Children with Neurodevelopmental Disorders
Authors: Caliendo Mariarosaria , Lanzara Valentina , Vetri Luigi , Roccella Michele , Marotta Rosa , Carotenuto Marco , Russo Daniela , Cerroni Francesco , Precenzano Francesco
Published: September 2020

Background and Objectives: Siblings of disabled children are more at risk of developing mental illnesses. More than 50 international studies show that about 8% of children and adolescents suffer from a mental disorder, which is almost always a source of difficulties both at the interpersonal level (in the family and with peers) and at school. Healthy siblings of children with disabilities are one of the groups most at risk for consequences in psychological health and well-being. As some authors suggest, siblings build their idea of “being people”, in terms of character and personality, by continuously and daily confronting themselves with the theme of disability and a family context subjected to continuous stress. The following contribution aims to compare emotional–behavioral disorders in healthy siblings of children with autism spectrum disorder, in healthy siblings of children with Down’s syndrome and in healthy siblings of children with typical development. Materials and Methods: The results involve 153 children from the region of Campania and their caregivers through the administration of the Strength and Difficulties Questionnaire. Results: From the data, it emerged that siblings of children with autism spectrum disorder and siblings of children with Down’s syndrome have a greater emotional fragility, especially among male subjects. Conclusions: Our results require us to reflect on the clinical and policy measures needed to ensure the well-being of siblings of disabled children, mainly through appropriate sibling coping training.
Emergency Medical Service Directors’ Protocols for Exertional Heat Stroke
Authors: Szymanski Michael R. , Scarneo-Miller Samantha E. , Smith M. Seth , Bruner Michelle L. , Casa Douglas J.
Published: September 2020

Background and Objectives: Emergency Medical Service (EMS) protocols vary widely and may not implement best practices for exertional heat stroke (EHS). EHS is 100% survivable if best practices are implemented within 30 min. The purpose of this study is to compare EMS protocols to best practices for recognizing and treating EHS. Materials and Methods: Individuals (n = 1350) serving as EMS Medical or Physician Director were invited to complete a survey. The questions related to the EHS protocols for their EMS service. 145 individuals completed the survey (response rate = 10.74%). Chi-Squared Tests of Associations (χ2) with 95% confidence intervals (CI) were calculated. Prevalence ratios (PR) with 95% CI were calculated to determine the prevalence of implementing best practices based on location, working with an athletic trainer, number of EHS cases, and years of directing. All PRs whose 95% CIs excluded 1.00 were considered statistically significant; Chi-Squared values with p values < 0.05 were considered statistically significant. Results: A majority of the respondents reported not using rectal thermometry for the diagnosis of EHS (n = 102, 77.93%) and not using cold water immersion for the treatment of EHS (n = 102, 70.34%). If working with an athletic trainer, EMS is more likely to implement best-practice treatment (i.e., cold-water immersion and cool-first transport-second) (69.6% vs. 36.9%, χ2 = 8.480, p < 0.004, PR = 3.15, 95% CI = 1.38, 7.18). Conclusions: These findings demonstrate a lack of implementation of best-practice standards for EHS by EMS. Working with an athletic trainer appears to increase the likelihood of following best practices. Efforts should be made to improve EMS providers’ implementation of best-practice standards for the diagnosis and management of EHS to optimize patient outcomes.
Survival of Patients on Hemodialysis with Erectile Dysfunction
Authors: Stolic Radojica V. , Bukumiric Zoran , Belic Branislav , Odalovic Bozidar , Relic Goran , Sovtic Sasa , Sipic Maja , Mitrovic Vekoslav , Krdzic Biljana
Published: September 2020

Background and objectives: In patients on hemodialysis, erectile dysfunction is an independent mortality factor. This study aimed to determine the risk factors that affect the survival of hemodialysis patients with erectile dysfunction. Materials and Methods: During a seven-year period, erectile dysfunction was identified among the fatalities reported in patients receiving chronic hemodialysis, on the basis of the International Index of Erectile Function questionnaire. The study covered 70 patients of mean age 57 ± 6.7 years. During the examined period, 42 (60%) patients died at the mean age 57 ± 6.8 years. The study was completed by 28 (40%) patients, aged 57 ± 6.55 years. Laboratory, demographic, anthropometric, and clinical characteristics were recorded using standard methods. Results: Statistically significant differences between the two groups of respondents were found concerning dialysis duration (p < 0.001), number of leukocytes (p = 0.003), adequacy of hemodialysis (p = 0.004), intima media thickness of the carotid artery (p < 0.001), presence of cardiovascular disease (p = 0.03), residual diuresis (p = 0.04), and hemodiafiltration (p < 0.001). Hemodialysis adequacy (B = −9.634; p = 0.017), intima media thickness (B = 0.022; p = 0.003), residual diuresis (B = −0.060; p = 0.007), and lower rates of cardiovascular disease (B = 0.176; p = 0.034) were significant survival predictors among our patients with erectile dysfunction. Conclusions: Risk factors that are associated with improved survival of patients on hemodialysis with erectile dysfunction in our study are: preserved diuresis, high-quality hemodialysis, lower incidence of cardiovascular disease, and less thickening of the intima media of the carotid arteries.
Increased Overall Heart Rate Irregularity Risk by Hyperuricemia in the General Population: Results from the Korean National Health and Nutrition Examination Survey
Authors: Eun Yeonghee , Han Kyung-Do , Kim Da Hye , Kim In Young , Park Eun-Jung , Lee Seulkee , Cha Hoon-Suk , Koh Eun-Mi , Lee Jaejoon , Kim Hyungjin
Published: September 2020

Background and objectives: Hyperuricemia is one of the well-known cardiovascular risk factors. There is a growing interest in the association between hyperuricemia and arrhythmia. We used the representative sample data of Korean population to study the association between hyperuricemia and heart rate irregularity (HRI) that reflects total arrhythmia. Materials and Methods: We performed weighted multivariate logistic regression analysis to assess the association between hyperuricemia and HRI. Results: Of the 10,827 subjects, 1308 (13.2%) had hyperuricemia and 130 (1%) had HRI. In the presence of hyperuricemia, HRI was three times higher than that in the absence of hyperuricemia (OR 2.98, 95% CI 1.71–5.18). The risk of HRI was highest in subjects with both hypertension and hyperuricemia. In the subgroup analysis, the association of hyperuricemia with HRI was most pronounced in the smoker group. Conclusions: Hyperuricemia was highly correlated with HRI in adult Korean representative sample data. Hyperuricemia was associated with a nearly tripled risk for HRI. Hypertension has a synergistic effect with hyperuricemia on HRI. Further research is warranted to clarify the relationship between hyperuricemia and arrhythmia and its mechanism.
Pepper Bacterial Spot Control by Bacillus velezensis: Bioprocess Solution
Authors: Pajčin Ivana , Vlajkov Vanja , Frohme Marcus , Grebinyk Sergii , Grahovac Mila , Mojićević Marija , Grahovac Jovana
Published: September 2020

Pepper bacterial spot is one of the most severe plant diseases in terms of infection persistence and economic losses when it comes to fresh pepper fruits used in nutrition and industrial processing. In this study, Bacillus velezensis IP22 isolated from fresh cheese was used as a biocontrol agent of pepper bacterial spot, whose main causal agent is the cosmopolitan pathogen Xanthomonas euvesicatoria. After optimization of the cultivation medium composition aimed at maximizing of the antimicrobial activity against X. euvesicatoria and validation of the optimized medium at the scale of a laboratory bioreactor, in planta tests were performed. The results have showed significant suppression of bacterial spot symptoms in pepper plants by the produced biocontrol agent, as well as reduction of disease spreading on the healthy (uninoculated) pepper leaves. Furthermore, HPLC-MS (high pressure liquid chromatography–mass spectrometry) analysis was employed to examine antimicrobial metabolites produced by B. velezensis IP22, where lipopeptides were found with similar m/z values compared to lipopeptides from fengycin and locillomycin families. The bioprocess solution developed at the laboratory scale investigated in this study represents a promising strategy for production of pepper bacterial spot biocontrol agent based on B. velezensis IP22, a food isolate with a great perspective for application in plant protection.
Evaluation of Clay Hydration and Swelling Inhibition Using Quaternary Ammonium Dicationic Surfactant with Phenyl Linker
Authors: Murtaza Mobeen , Ahmad Hafiz Mudaser , Kamal Muhammad Shahzad , Hussain Syed Muhammad Shakil , Mahmoud Mohamed , Patil Shirish
Published: September 2020

Water-based drilling fluids are extensively used for drilling oil and gas wells. However, water-based muds cause clay swelling, which severely affects the stability of wellbore. Due to two adsorption positions, it is expected that cationic gemini surfactants can reduce the clay swelling. In this work, quaternary ammonium dicationic gemini surfactants containing phenyl linkers and different counterions (Cl− and Br−) were synthesized, and the effect of variation in counterions on swelling and hydration properties of shales was studied. Numerous water-based drilling fluid formulations were prepared with different concentrations of surfactants to study the swelling inhibition capacity of surfactants. The performance of surfactant-containing drilling muds was evaluated by comparing them with base drilling mud, and sodium silicate drilling mud. Various experimental techniques were employed to study drilling mud characteristics such as rheology and filtration. The inhibition properties of drilling mud formulations were determined by linear swelling experiment, capillary suction time test, particle size distribution measurement, wettability measurements, and X-ray Diffraction (XRD). Experimental results showed that surfactant-based formulation containing bromide counterion exhibited superior rheological properties as compared to other investigated formulations. The filtration test showed that the gemini surfactant with chloride counterion had higher filtrate loss compared to all other formulations. The bentonite swelling was significantly reduced with increasing the concentration of dicationic surfactants as inhibitors, and maximum reduction in the linear swelling rate was observed by using a formulation containing surfactant with chloride counterion. The lowest capillary suction timer (CST) was obtained in the formulation containing surfactant with chloride counterion as less CST indicated the enhanced inhibition capacity. The particle size measurement showed that average bentonite particle size increased upon the addition of surfactants depicting the inhibition capacity. The increase in basal spacing obtained from XRD analysis showed the intercalation of gemini surfactants in interlayers of bentonite. The contact angle measurements were performed to study the wettability of the bentonite film surface, and the results showed that hydrophobicity increased by incorporating the surfactants to the drilling fluid.
Isolation and FTIR-ATR and 1H NMR Characterization of Alginates from the Main Alginophyte Species of the Atlantic Coast of Morocco
Authors: Belattmania Zahira , Kaidi Soukaina , El Atouani Samir , Katif Chaimaa , Bentiss Fouad , Jama Charafeddine , Reani Abdeltif , Sabour Brahim , Vasconcelos Vitor
Published: September 2020

Alginates are widely used as gelling agents in textile print pastes, medical industries, impression material in dentistry, and anticoagulant material in toothpaste. In the present study, the content and spectroscopic characterization (1H NMR and FT-IR) of the sodium alginates were investigated in the eight brown seaweeds Sargassum muticum, Fucus vesiculosus f. volubilis, Carpodesmia tamariscifolia, Bifurcaria bifurcata, Laminaria ochroleuca, Cystoseira humilis, Saccorhiza polyschides, and Fucus guiryi harvested from the NW Atlantic coast of Morocco. The results proved that the most studied algae depicted alginate yields higher than 18% dry weight. The FT-IR analysis showed that the spectra of the extracted alginates exhibited significant similarities to the commercial alginate from Sigma-Aldrich. The 1H NMR spectroscopy indicated that the extracted alginates have a high content of β-d-mannuronic (M) than α-l-guluronic acid (G) with M/G ratio values ranging from 1.04 to 4.41. The homopolymeric fractions FMM are remarkably high compared to the FGG and heteropolymeric fractions (FGM = FMG) especially for F. guiryi, C humilis, C. tamariscifolia, L. ochroleuca, and S. polyschides. Nevertheless, the heteropolymeric fractions (FGM/FMG) are quite abundant in the alginates of S. muticum, F. vesiculosus f. volubilis, and B. bifurcata accounting for more than 52% of the polymer diads. Based on these results, the investigated algal species (except Fucus guiryi and Bifurcaria bifurcata) could be regarded as potential sources of alginates for industrial uses.
Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity
Authors: Kostić Marina , Ivanov Marija , Fernandes Ângela , Pinela José , Calhelha Ricardo C. , Glamočlija Jasmina , Barros Lillian , Ferreira Isabel C. F. R. , Soković Marina , Ćirić Ana
Published: September 2020

This study explored the biological properties of three wild growing Russula species (R. integra, R. rosea, R. nigricans) from Serbia. Compositional features and antioxidant, antibacterial, antibiofilm, and cytotoxic activities were analyzed. The studied mushroom species were identified as being rich sources of carbohydrates and of low caloric value. Mannitol was the most abundant free sugar and quinic and malic acids the major organic acids detected. The four tocopherol isoforms were found, and polyunsaturated fatty acids were the predominant fat constituents. Regarding phenolic compounds, P-hydroxybenzoic and cinnamic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation and oxidative hemolysis; the highest activity was attributed to the R. nigricans ethanolic extract. This is the first report on the antibacterial and antibiofilm potential of the studied species, with the most promising activity observed towards Streptococcus spp. (0.20–0.78 mg/mL as the minimal inhibitory concentration, MIC). The most promising cytotoxic effect was caused by the R. integra methanolic extract on non-small cell lung cancer cells (NCI-H460). Therefore, due to the observed in vitro bioactive properties, the studied mushrooms arise as a source of functional ingredients with potential to be used in novel nutraceutical and drug formulations, which can be used in the treatment of various diseases and health conditions.
Sulphamoylated Estradiol Analogue Induces Reactive Oxygen Species Generation to Exert Its Antiproliferative Activity in Breast Cancer Cell Lines
Authors: Lebelo Maphuti T. , Joubert Anna M. , Visagie Michelle H.
Published: September 2020

2-Methoxyestradiol (2ME), a 17β-estradiol metabolite, exerts anticancer properties in vitro and in vivo. To address 2ME’s low bioavailability, research led to the in silico design of sulphamoylated 2ME analogues. However, the role of oxidative stress induced in the activity exerted by sulphamoylated compounds remains elusive. In the current study, the influence of 2-Ethyl-17-oxoestra-1,3,5(10)-trien-3-yl sulphamate (ESE-one) on reactive oxygen species (ROS) induction and its effect on cell proliferation, as well as morphology, were assessed in breast tumorigenic cells (MCF-7 and MDA-MB-231). Fluorescent microscopy showed that sulphamoylated estradiol analogues induced hydrogen peroxide and superoxide anion, correlating with decreased cell growth demonstrated by spectrophotometry data. ESE-one exposure resulted in antiproliferation which was repressed by tiron (superoxide inhibitor), trolox (peroxyl inhibitor) and N,N′-dimethylthiourea (DMTU) (hydrogen peroxide inhibitor). Morphological studies demonstrated that tiron, trolox and DMTU significantly decreased the number of rounded cells and shrunken cells in MCF-7 and MDA-MB-231 cells induced by ESE-one. This in vitro study suggests that ESE-one induces growth inhibition and cell rounding by production of superoxide anion, peroxyl radical and hydrogen peroxide. Identification of these biological changes in cancer cells caused by sulphamoylated compounds hugely contributes towards improvement of anticancer strategies and the ROS-dependent cell death pathways in tumorigenic breast cells.
Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production
Authors: Jaramillo David A. , Méndez María J. , Vargas Gabriela , Stashenko Elena E. , Vasco-Palacios Aída-M. , Ceballos Andrés , Caicedo Nelson H.
Published: September 2020

Aromas and flavours can be produced from fungi by either de novo synthesis or biotransformation processes. Herein, the biocatalytic potential of seven basidiomycete species from Colombia fungal strains isolated as endophytes or basidioma was evaluated. Ganoderma webenarium, Ganoderma chocoense, and Ganoderma stipitatum were the most potent strains capable of decolourizing β,β-carotene as evidence of their potential as biocatalysts for de novo aroma synthesis. Since a species’ biocatalytic potential cannot solely be determined via qualitative screening using β,β-carotene biotransformation processes, we focused on using α-pinene biotransformation with mycelium as a measure of catalytic potential. Here, two strains of Trametes elegans—namely, the endophytic (ET-06) and basidioma (EBB-046) strains—were screened. Herein, T. elegans is reported for the first time as a novel biocatalyst for the oxidation of α-pinene, with a product yield of 2.9 mg of cis-Verbenol per gram of dry weight mycelia used. The EBB-046 strain generated flavour compounds via the biotransformation of a Cape gooseberry medium and de novo synthesis in submerged cultures. Three aroma-producing compounds were identified via GC–MS—namely, methyl-3-methoxy-4H-pyran-4-one, hexahydro-3-(methylpropyl)-pyrrolo[1,2-a]pyrazine-1,4-dione, and hexahydro-3-(methylphenyl)-pyrrolo[1,2-a]pyrazine-1,4-dione.