bluuebunny/crossref_metadata_2025 huggingface dataset:
B-Cell Receptor-Associated Protein 31 Promotes Metastasis via AKT/β-Catenin/Snail Pathway in Hepatocellular Carcinoma
Authors: Liu Tengfei , Yu Junming , Ge Chao , Zhao Fangyu , Miao Chunxiao , Jin Wenjiao , Su Yang , Geng Qin , Chen Taoyang , Xie Haiyang , Cui Ying , Yao Ming , Li Jinjun , Hou Helei , Li Hong
Published: June 2021

Hepatocellular carcinoma (HCC) is one of the most lethal cancer worldwide, characterized with high heterogeneity and inclination to metastasize. Emerging evidence suggests that BAP31 gets involved in cancer progression with different kinds. It still remains unknown whether and how BAP31 plays a role in HCC metastasis. Epithelial–mesenchymal transition (EMT) has been a common feature in tumor micro-environment, whose inducer TGF-β increased BAP31 expression in this research. Elevated expression of BAP31 was positively correlated with tumor size, vascular invasion and poor prognosis in human HCC. Ectopic expression of BAP31 promoted cell migration and invasion while BAP31 knockdown markedly attenuated metastatic potential in HCC cells and mice orthotopic xenografts. BAP31 induced EMT process, and enhanced the expression level of EMT-related factor Snail and decreased contents and membrane distribution of E-cadherin. BAP31 also activated AKT/β-catenin pathway, which mediated its promotional effects on HCC metastasis. AKT inhibitor further counteracted the activated AKT/β-catenin/Snail upon BAP31 over-expression. Moreover, silencing Snail in BAP31-overexpressed cells impaired enhanced migratory and invasive abilities of HCC cells. In HCC tissues, BAP31 expression was positively associated with Snail. In conclusion, BAP31 promotes HCC metastasis by activating AKT/β-catenin/Snail pathway. Thus, our study implicates BAP31 as potential prognostic biomarker, and provides valuable information for HCC prognosis and treatment.
Thermodynamic Origin of Differential Excipient-Lysozyme Interactions
Authors: Kalayan Jas , Curtis Robin A. , Warwicker Jim , Henchman Richard H.
Published: June 2021

Understanding the intricate interplay of interactions between proteins, excipients, ions and water is important to achieve the effective purification and stable formulation of protein therapeutics. The free energy of lysozyme interacting with two kinds of polyanionic excipients, citrate and tripolyphosphate, together with sodium chloride and TRIS-buffer, are analysed in multiple-walker metadynamics simulations to understand why tripolyphosphate causes lysozyme to precipitate but citrate does not. The resulting multiscale decomposition of energy and entropy components for water, sodium chloride, excipients and lysozyme reveals that lysozyme is more stabilised by the interaction of tripolyphosphate with basic residues. This is accompanied by more sodium ions being released into solution from tripolyphosphate than for citrate, whilst the latter instead has more water molecules released into solution. Even though lysozyme aggregation is not directly probed in this study, these different mechanisms are suspected to drive the cross-linking between lysozyme molecules with vacant basic residues, ultimately leading to precipitation.
Multidisciplinary Tinnitus Research: Challenges and Future Directions From the Perspective of Early Stage Researchers
Authors: Simoes Jorge Piano , Daoud Elza , Shabbir Maryam , Amanat Sana , Assouly Kelly , Biswas Roshni , Casolani Chiara , Dode Albi , Enzler Falco , Jacquemin Laure , Joergensen Mie , Kok Tori , Liyanage Nuwan , Lourenco Matheus , Makani Punitkumar , Mehdi Muntazir , Ramadhani Anissa L. , Riha Constanze , Santacruz Jose Lopez , Schiller Axel , Schoisswohl Stefan , Trpchevska Natalia , Genitsaridi Eleni
Published: June 2021

Tinnitus can be a burdensome condition on both individual and societal levels. Many aspects of this condition remain elusive, including its underlying mechanisms, ultimately hindering the development of a cure. Interdisciplinary approaches are required to overcome long-established research challenges. This review summarizes current knowledge in various tinnitus-relevant research fields including tinnitus generating mechanisms, heterogeneity, epidemiology, assessment, and treatment development, in an effort to highlight the main challenges and provide suggestions for future research to overcome them. Four common themes across different areas were identified as future research direction: (1) Further establishment of multicenter and multidisciplinary collaborations; (2) Systematic reviews and syntheses of existing knowledge; (3) Standardization of research methods including tinnitus assessment, data acquisition, and data analysis protocols; (4) The design of studies with large sample sizes and the creation of large tinnitus-specific databases that would allow in-depth exploration of tinnitus heterogeneity.
The Unfolded Protein Response in Immune Cells as an Emerging Regulator of Neuroinflammation
Authors: Fernández Dominique , Geisse Antonia , Bernales Jose Ignacio , Lira Alonso , Osorio Fabiola
Published: June 2021

Immune surveillance is an essential process that safeguards the homeostasis of a healthy brain. Among the increasing diversity of immune cells present in the central nervous system (CNS), microglia have emerged as a prominent leukocyte subset with key roles in the support of brain function and in the control of neuroinflammation. In fact, impaired microglial function is associated with the development of neurodegenerative diseases, including Alzheimer’s disease (AD) and Parkinson’s disease (PD). Interestingly, these pathologies are also typified by protein aggregation and proteostasis dysfunction at the level of the endoplasmic reticulum (ER). These processes trigger activation of the unfolded protein response (UPR), which is a conserved signaling network that maintains the fidelity of the cellular proteome. Remarkably, beyond its role in protein folding, the UPR has also emerged as a key regulator of the development and function of immune cells. However, despite this evidence, the contribution of the UPR to immune cell homeostasis, immune surveillance, and neuro-inflammatory processes remains largely unexplored. In this review, we discuss the potential contribution of the UPR in brain-associated immune cells in the context of neurodegenerative diseases.
What Is Learned in Pavlovian Conditioning in Crickets? Revisiting the S-S and S-R Learning Theories
Authors: Mizunami Makoto
Published: June 2021

In Pavlovian conditioning in mammals, two theories have been proposed for associations underlying conditioned responses (CRs). One theory, called S-S theory, assumes an association between a conditioned stimulus (CS) and internal representation of an unconditioned stimulus (US), allowing the animal to adjust the CR depending on the current value of the US. The other theory, called S-R theory, assumes an association or connection between the CS center and the CR center, allowing the CS to elicit the CR. Whether these theories account for Pavlovian conditioning in invertebrates has remained unclear. In this article, results of our studies in the cricket Gryllus bimaculatus are reviewed. We showed that after a standard amount of Pavlovian training, crickets exhibited no response to odor CS when water US was devalued by providing it until satiation, whereas after extended training, they exhibited a CR after US devaluation. An increase of behavioral automaticity by extended training has not been reported in Pavlovian conditioning in any other animals, but it has been documented in instrumental conditioning in mammals. Our pharmacological analysis suggested that octopamine neurons mediate US (water) value signals and control execution of the CR after standard training. The control, however, diminishes with extension of training and hence the CR becomes insensitive to the US value. We also found that the nature of the habitual response after extended Pavlovian training in crickets is not the same as that after extended instrumental training in mammals concerning the context specificity. Adaptive significance and evolutionary implications for our findings are discussed.
Natural and Experimental Evidence Drives Marmosets for Research on Psychiatric Disorders Related to Stress
Authors: de Sousa Maria Bernardete Cordeiro , de Meiroz Grilo Maria Lara Porpino , Galvão-Coelho Nicole Leite
Published: June 2021

Knowledge of the behavioral ecology of marmosets carried out in their natural habitat associated with the advent of a non-invasive technique for measuring steroid hormones in feces has made a significant contribution to understanding their social relationships and sexual strategies. These studies showed that they are mainly monogamous, live in relatively stable social groups according to a social hierarchy in which females compete and males cooperate, and form social bonds similar to humans, which makes this species a potential animal model to study disorders related to social stress. In addition, laboratory studies observed the expression of behaviors similar to those in nature and deepened the descriptions of their social and reproductive strategies. They also characterized their responses to the challenge using behavioral, cognitive, physiological, and genetic approaches that were sexually dimorphic and influenced by age and social context. These findings, added to some advantages which indicate good adaptation to captivity and the benefits of the birth of twins, small size, and life cycle in comparison to primates of the Old World, led to their use as animal models for validating psychiatric diseases such as major depression. Juvenile marmosets have recently been used to develop a depression model and to test a psychedelic brew called Ayahuasca from the Amazon rainforest as an alternative treatment for major depression, for which positive results have been found which encourage further studies in adolescents. Therefore, we will review the experimental evidence obtained so far and discuss the extension of the marmoset as an animal model for depression.
The Gain-of-Function p53 R248W Mutant Promotes Migration by STAT3 Deregulation in Human Pancreatic Cancer Cells
Authors: Klemke Luisa , Fehlau Clara F. , Winkler Nadine , Toboll Felicia , Singh Shiv K. , Moll Ute M. , Schulz-Heddergott Ramona
Published: June 2021

Missense p53 mutations (mutp53) occur in approx. 70% of pancreatic ductal adenocarcinomas (PDAC). Typically, mutp53 proteins are aberrantly stabilized by Hsp90/Hsp70/Hsp40 chaperone complexes. Notably, stabilization is a precondition for specific mutp53 alleles to acquire powerful neomorphic oncogenic gain-of-functions (GOFs) that promote tumor progression in solid cancers mainly by increasing invasion and metastasis. In colorectal cancer (CRC), we recently established that the common hotspot mutants mutp53R248Q and mutp53R248W exert GOF activities by constitutively binding to and hyperactivating STAT3. This results in increased proliferation and invasion in an autochthonous CRC mouse model and correlates with poor survival in patients. Comparing a panel of p53 missense mutations in a series of homozygous human PDAC cell lines, we show here that, similar to CRC, the mutp53R248W protein again undergoes a strong Hsp90-mediated stabilization and selectively promotes migration. Highly stabilized mutp53 is degradable by the Hsp90 inhibitors Onalespib and Ganetespib, and correlates with growth suppression, possibly suggesting therapeutic vulnerabilities to target GOF mutp53 proteins in PDAC. In response to mutp53 depletion, only mutp53R248W harboring PDAC cells show STAT3 de-phosphorylation and reduced migration, again suggesting an allele-specific GOF in this cancer entity, similar to CRC. Moreover, mutp53R248W also exhibits the strongest constitutive complex formation with phosphorylated STAT3. The selective mutp53R248W GOF signals through enhancing the STAT3 axis, which was confirmed since targeting STAT3 by knockdown or pharmacological inhibition phenocopied mutp53 depletion and reduced cell viability and migration preferentially in mutp53R248W-containing PDAC cells. Our results confirm that mutp53 GOF activities are allele specific and can span across tumor entities.
Retropharyngeal Lymph Node Metastasis Diagnosed by Magnetic Resonance Imaging in Hypopharyngeal Carcinoma: A Retrospective Analysis From Chinese Multi-Center Data
Authors: An Changming , Sun Ying , Miao Susheng , Yu Xiaoduo , Zhang Ye , Zhang Xiwei , Xia Lili , Liu Shaoyan , Li Zhengjiang , Yi Junlin
Published: June 2021

Background

To assess the prevalence, risk factors and prognostic significance of retropharyngeal lymph node (RPLN) metastasis diagnosed by magnetic resonance imaging (MRI) in patients with hypopharyngeal squamous cell carcinoma (HPSCC).

Methods

259 patients from three cancer institutions in China from Jan 2010 to Dec 2018 were analyzed, retrospectively. All the patients had been given pre-treatment magnetic resonance imaging (MRI) of head and neck and were then treated with definitive radiotherapy with or without chemotherapy. Pretreatment diagnostic MRIs were reviewed by a dedicated head and neck radiologist, for the presence or absence of radiographically positive RPLN, cervical LN and tumor invasion.Demographic variables were analysed by descriptive statistics using SPSS 20.0. Predictors of the presence of RPLN and its prognostic significance were examined.

Results

RPLN metastasis was discovered in 44 patients (17%). Logistic analysis showed that posterior pharyngeal wall (PPW) primary tumor; PPW invasion; N2-3; multiple cervical lymph node (LN) involvement (>2 LNs) were associated with RPLN metastasis, with metastasis rates 37%, 30%, 31% and 33% respectively. Patients with RPLN metastasis had a significantly reduced 5-year overall survival (OS) and disease-free survival (DFS) compared to the non-RPLN metastasis group (OS 28% vs. 48%, p=0.001; DFS 25% vs. 41%, p=0.040).

Conclusions

RPLN metastasis was not uncommon in HPSCC patients. Risk factors were: PPW primary tumor, PPW invasion and cervical LN status. RPLN metastasis is a poor prognosticator for survival.
Prevalence and Spectrum of Germline BRCA1 and BRCA2 Variants of Uncertain Significance in Breast/Ovarian Cancer: Mysterious Signals From the Genome
Authors: Fanale Daniele , Fiorino Alessia , Incorvaia Lorena , Dimino Alessandra , Filorizzo Clarissa , Bono Marco , Cancelliere Daniela , Calò Valentina , Brando Chiara , Corsini Lidia Rita , Sciacchitano Roberta , Magrin Luigi , Pivetti Alessia , Pedone Erika , Madonia Giorgio , Cucinella Alessandra , Badalamenti Giuseppe , Russo Antonio , Bazan Viviana
Published: June 2021

About 10–20% of breast/ovarian (BC/OC) cancer patients undergoing germline BRCA1/2 genetic testing have been shown to harbor Variants of Uncertain Significance (VUSs). Since little is known about the prevalence of germline BRCA1/2 VUS in Southern Italy, our study aimed at describing the spectrum of these variants detected in BC/OC patients in order to improve the identification of potentially high-risk BRCA variants helpful in patient clinical management. Eight hundred and seventy-four BC or OC patients, enrolled from October 2016 to December 2020 at the “Sicilian Regional Center for the Prevention, Diagnosis and Treatment of Rare and Heredo-Familial Tumors” of University Hospital Policlinico “P. Giaccone” of Palermo, were genetically tested for germline BRCA1/2 variants through Next-Generation Sequencing analysis. The mutational screening showed that 639 (73.1%) out of 874 patients were BRCA-w.t., whereas 67 (7.7%) were carriers of germline BRCA1/2 VUSs, and 168 (19.2%) harbored germline BRCA1/2 pathogenic/likely pathogenic variants. Our analysis revealed the presence of 59 different VUSs detected in 67 patients, 46 of which were affected by BC and 21 by OC. Twenty-one (35.6%) out of 59 variants were located on BRCA1 gene, whereas 38 (64.4%) on BRCA2. We detected six alterations in BRCA1 and two in BRCA2 with unclear interpretation of clinical significance. Familial anamnesis of a patient harboring the BRCA1-c.3367G>T suggests for this variant a potential of pathogenicity, therefore it should be carefully investigated. Understanding clinical significance of germline BRCA1/2 VUS could improve, in future, the identification of potentially high-risk variants useful for clinical management of BC or OC patients and family members.
Student-Designed Cross-Sectional Pandemic Knowledge Survey of 8th−12th Grade Students, Milwaukee, WI, April 2020
Authors: Vasudev Krish , Singh Hersh , Neumann August A. , Zhao William M , Mondano Lyle Dominic Pelayo
Published: June 2021

Background: The novel coronavirus and its effect on our society are unprecedented. Given the recent pandemic, numerous measures have been taken to protect our communities. We sought to understand our school community's knowledge and the measures that were taken by our school for our safety.

Objective: Our objective was to describe the overall understanding and attitudes of 8–12th grade students from a single institution during the initial phase of the Wisconsin's Governor's stay-at-home order.

Methods: A voluntary web-based survey was communicated to 8–12th grade students through their online school portal. Data were collected and analyzed using SurveyMonkey.

Results: There was a 20.2% response rate. Answers regarding the coronavirus, spread, and response to the coronavirus pandemic showed a high level of understanding of the virus and the actions necessary to prevent its spread.

Conclusion: Eight-twelfth grade students have a high level of understanding of the virus, its effects, and the safety measures implemented to protect society.
Dose–Response Effect of Vibratory Stimulus on Synaptic and Muscle Plasticity in a Middle-Aged Murine Model
Authors: Cariati Ida , Bonanni Roberto , Annino Giuseppe , Scimeca Manuel , Bonanno Elena , D’Arcangelo Giovanna , Tancredi Virginia
Published: June 2021

Whole body vibration plays a central role in many work categories and can represent a health risk to the musculoskeletal system and peripheral nervous system. However, studies in animal and human models have shown that vibratory training, experimentally and/or therapeutically induced, can exert beneficial effects on the whole body, as well as improve brain functioning and reduce cognitive decline related to the aging process. Since the effects of vibratory training depend on several factors, such as vibration frequency and vibration exposure time, in this work, we investigated whether the application of three different vibratory protocols could modulate synaptic and muscle plasticity in a middle-aged murine model, counteracting the onset of early symptoms linked to the aging process. To this end, we performed in vitro electrophysiological recordings of the field potential in the CA1 region of mouse hippocampal slices, as well as histomorphometric and ultrastructural analysis of muscle tissue by optic and transmission electron microscopy, respectively. Our results showed that protocols characterized by a low vibration frequency and/or a longer recovery time exert positive effects at both hippocampal and muscular level, and that these effects improve significantly by varying both parameters, with an action comparable with a dose–response effect. Thus, we suggested that vibratory training may be an effective strategy to counteract cognitive impairment, which is already present in the early stages of the aging process, and the onset of sarcopenia, which is closely related to a sedentary lifestyle. Future studies are needed to understand the underlying molecular mechanisms and to determine an optimal vibratory training protocol.
Applications and Major Achievements of Genome Editing in Vegetable Crops: A Review
Authors: Kim Young-Cheon , Kang Yeeun , Yang Eun-Young , Cho Myeong-Cheoul , Schafleitner Roland , Lee Jeong Hwan , Jang Seonghoe
Published: June 2021

The emergence of genome-editing technology has allowed manipulation of DNA sequences in genomes to precisely remove or replace specific sequences in organisms resulting in targeted mutations. In plants, genome editing is an attractive method to alter gene functions to generate improved crop varieties. Genome editing is thought to be simple to use and has a lower risk of off-target effects compared to classical mutation breeding. Furthermore, genome-editing technology tools can also be applied directly to crops that contain complex genomes and/or are not easily bred using traditional methods. Currently, highly versatile genome-editing tools for precise and predictable editing of almost any locus in the plant genome make it possible to extend the range of application, including functional genomics research and molecular crop breeding. Vegetables are essential nutrient sources for humans and provide vitamins, minerals, and fiber to diets, thereby contributing to human health. In this review, we provide an overview of the brief history of genome-editing technologies and the components of genome-editing tool boxes, and illustrate basic modes of operation in representative systems. We describe the current and potential practical application of genome editing for the development of improved nutritious vegetables and present several case studies demonstrating the potential of the technology. Finally, we highlight future directions and challenges in applying genome-editing systems to vegetable crops for research and product development.
Associations Between Resilience, Psychological Well-Being, Work-Related Stress and Covid-19 Fear in Forensic Healthcare Workers Using a Network Analysis
Authors: Bogaerts Stefan , van Woerkom Marianne , Erbaş Yasemin , De Caluwé Elien , Garofalo Carlo , Frowijn Iris , Jeandarme Ingeborg , Masthoff Erik , Janković Marija
Published: June 2021

Forensic healthcare workers deal with patients with severe psychiatric and behavioral problems that put them at an increased risk of developing work-related stress and burnout. Working with this target group of patients during the Coronavirus disease 2019 (Covid-19) pandemic with far-reaching restrictive measures can negatively affect the psychological well-being of forensic workers. Research suggests that resilience can buffer workplace stress and contribute positively to psychological well-being. However, research on resilience, psychological well-being and work-related stress among forensic healthcare workers is still lacking. Therefore, in this study, we investigated the interrelations between psychological well-being and resilience on the one hand and work-related stress and Covid-19 fear-related symptoms on the other hand. Self-report data were obtained from 318 healthcare workers (73.9% women) working in three Forensic Psychiatric Centers (M age = 44.20, SD = 14.31) and are in direct contact with forensic patients. The data were analyzed using network analysis. Consistent with previous research, the results showed that workplace stress and fear associated with the Covid-19 pandemic can be detrimental to workers' psychological well-being, while resilience can serve as a protective factor against being personally attacked or threatened by patients at the workplace. Last but not least, we identified highly central symptoms, namely tremors due to the fear of the coronavirus and anxiety when other people coughing, which would be the best candidates for future treatment targets. This knowledge can help clinicians optimize interventions to reduce workplace stress and fear due to the pandemic. Future studies should aim to replicate our findings in a larger and more representative sample of forensic healthcare workers.
GT198 Is a Target of Oncology Drugs and Anticancer Herbs
Authors: Pang Junfeng , Gao Jie , Zhang Liyong , Mivechi Nahid F. , Ko Lan
Published: June 2021

Tumor angiogenesis is a hallmark of cancer. Therapeutic drug inhibitors targeting angiogenesis are clinically effective. We have previously identified GT198 (gene symbol PSMC3IP, also known as Hop2) as an oncoprotein that induces tumor angiogenesis in human cancers, including oral cancer. In this study, we show that the GT198 protein is a direct drug target of more than a dozen oncology drugs and several clinically successful anticancer herbs. GT198 is a DNA repair protein that binds to DNA. Using an in vitro DNA-binding assay, we tested the approved oncology drug set VII from the National Cancer Institute containing 129 oncology drugs. Identified GT198 inhibitors include but are not limited to mitoxantrone, doxorubicin, paclitaxel, etoposide, dactinomycin, and imatinib. Paclitaxel and etoposide have higher binding affinities, whereas doxorubicin has higher binding efficacy due to competitive inhibition. GT198 shares protein sequence homology with DNA topoisomerases, which are known drug targets, so that GT198 is likely a new drug target previously unrecognized. To seek more powerful GT198 inhibitors, we further tested several anticancer herbal extracts. The positive anticancer herbs with high affinity and high efficacy are all clinically successful ones, including allspice from Jamaica, Gleditsia sinensis or honey locust from China, and BIRM from Ecuador. Partial purification of allspice using an organic chemical approach demonstrated great feasibility of natural product purification, when the activity is monitored by the in vitro DNA-binding assay using GT198 as a target. Together, our study reveals GT198 as a new targeting mechanism for existing oncology drugs. The study also delivers an excellent drug target suitable for compound identification and natural product purification. In particular, this study opens an opportunity to rapidly identify drugs with high efficacy and low toxicity from nature.
Response of Peanut Varieties to Phosphorus and Rhizobium Inoculant Rates on Haplic Lixisols of Guinea Savanna Zone of Ghana
Authors: Naabe Yaro Richard , Rufai Mahama Ahmed , Kugbe Joseph Xorse , Berdjour Albert
Published: June 2021

Peanut forms a major component of the predominantly cereal-based farming systems in Northern Ghana. However, yields are low, prompting the need to evaluate the effects of phosphorus (PR) and rhizobium inoculant (IR) rates on growth, nodulation, and yield of peanut varieties. On-station and on-farm experiments were conducted to determine the interaction effects of three P rates (0, 30, and 60 kg P2O5/ha), three IR rates (0, 3, and 6 g/kg seed), and two peanut varieties [Chinese and Nkatie Savannah Agricultural Research Institute (SARI)] on growth, nodulation, and yield of peanut on Haplic Lixisols of Northern Ghana. Both experiments were conducted using a split-split plot design replicated three times for the on-station experiment and on six farmer's fields (on-farm experiment). In both experiments, combined application of 60 kg P2O5/ha and IR at 6 g/kg seed increased pod number in the Nkatie SARI and Chinese varieties compared to their control counterparts. PR × V interaction influenced growth, effective nodule number, and podding capacity with 60 kg P2O5/ha combined with Nkatie SARI to produce significantly higher values. The interaction of IR × V improved pod number, nodule number, and harvest index, such that inoculant at 6 g/kg seed combined with Nkatie SARI gave the best performance. PR × IR also had a significant interactive influence on peanut grain yield. Higher grain yields were recorded from 60 kg P2O5/ha in combination with 6 g/kg seed of rhizobium inoculant. Therefore, these results suggest that the use of P fertilizer at 60 kg/ha and rhizobium inoculant at 6 g/kg seed increase peanut productivity on Haplic Lixisols in Northern Ghana. However, it was prudent not to suggest any recommendations from the P rates in interaction with IR, since the result between the on-station and on-farm experiments appeared not consistent.
Evaluation of Scenarios for Improving the Collection System for a Milk Factory in Ethiopia
Authors: Dijkink Bert , Esveld Erik , Broeze Jan , Vollebregt Martijntje
Published: June 2021

The milk for a factory in Sululta (Ethiopia) is currently collected at ambient temperature. To increase milk production, the sourcing must be extended. This requires the collection of not only the morning milk but also the evening milk from smallholder farms. To accomplish this, the collection of milk from small farmers has to be improved, whereby the milk quality has to be assured with reasonable cost and environmental impact. A model predicting milk rejection was developed based on initial contamination and time and temperature profiles. With this model, different cooling scenarios we reevaluated regarding the expected effectiveness of reducing the rejection rate during collection. Second, cost estimations were made to implement the scenarios to collect morning and evening milk from smallholder farms. A third criterion was greenhouse gas (GHG) emissions per litre of collected milk. Finally, the feasibility of the scenarios was assessed in terms of technical, practical, and economic aspects. Including both quality and economics, the best scenario can be expected from a cooling centre where farmers bring their milk twice a day, except there are signals that the farmers would not be willing to deliver the evening milk to the centre at night. In that case, an additional collecting system would be needed to increase the milk supply. This would result in higher collection costs and an increased risk of milk rejection at the factory gate. Furthermore, this would reduce the value of the chilling centre, as in that case it would be better to deliver the milk directly to the factory. Both scenarios would increase GHG emissions compared with the current situation. Only the use of an off-grid solar power-driven cooling system at the farms would reduce the GHG emissions. However, this solution is less feasible economically. The applied combination of a simple model, economic analysis and the effect on GHG emissions gives valuable information on the effectiveness and limitations of different cooling scenarios for the milk factory. It can help to successfully apply a scenario for increasing the milk supply.
Biofumigation With Pellets of Defatted Seed Meal of Brassica carinata: Factors Affecting Performance Against Phytophthora nicotianae in Pepper Crops
Authors: Serrano-Pérez Paula , De Santiago Ana , Rodríguez-Molina María del Carmen
Published: June 2021

Phytophthora nicotianaeis the pathogen that causes root and crown rot disease in open field paprika pepper crops of Extremadura (central-western Spain). A field experiment was established during spring, a period compatible with the pepper crop cycle in this region, to evaluate the effects of biofumigation with pellets ofBrassica carinatadefatted seed meal, at the rate recommended by the manufacturer (3 tons ha−1), on the survival and infectivity ofP. nicotianaechlamydospores inoculum. Furthermore, three biofumigant rates (3, 6, and 20 tons ha−1) were assayed in laboratory experiments with natural soil inoculated with chlamydospores. In the field trial, the incorporation of pellets at 3 tons ha−1did not produce remarkable changes in soil enzyme activity or soil properties. In both the field and laboratory tests, survival and infectivity in the biofumigated treatment at 3 tons ha−1did not differ from those in the untreated control. On the contrary, the same rate added to autoclaved soil completely suppressed the inoculum, suggesting that the soil microbiota degradesB. carinatapellets before being effective againstP. nicotianae. Increasing the recommended rate to 6 tons ha−1decreased inoculum survival in the laboratory test and 20 tons ha−1completely inhibited the inoculum, although the economic value of such a high rate application is a factor to be assessed. In phytotoxicity tests on radish and white mustard seeds with several concentrations (100, 50, 25, 10, 5, and 0%) ofB. carinatapellets solution, no phytotoxic effect was observed with the two lowest concentrations, and no symptoms of phytotoxicity were found in the bioassays of pepper plants.
Day-1 Competencies for Veterinarians Specific to Health Informatics
Authors: Ouyang Zenhwa Ben , Hodgson Jennifer Louise , Robson Elliot , Havas Kevin , Stone Elizabeth , Poljak Zvonimir , Bernardo Theresa Marie
Published: June 2021

In 2015, the American Association of Veterinary Medical Colleges (AAVMC) developed the Competency-Based Veterinary Education (CBVE) framework to prepare practice-ready veterinarians through competency-based education, which is an outcomes-based approach to equipping students with the skills, knowledge, attitudes, values, and abilities to do their jobs. With increasing use of health informatics (HI: the use of information technology to deliver healthcare) by veterinarians, competencies in HI need to be developed. To reach consensus on a HI competency framework in this study, the Competency Framework Development (CFD) process was conducted using an online adaptation of Developing-A-Curriculum, an established methodology in veterinary medicine for reaching consensus among experts. The objectives of this study were to (1) create an HI competency framework for new veterinarians; (2) group the competency statements into common themes; (3) map the HI competency statements to the AAVMC competencies as illustrative sub-competencies; (4) provide insight into specific technologies that are currently relevant to new veterinary graduates; and (5) measure panelist satisfaction with the CFD process. The primary emphasis of the final HI competency framework was that veterinarians must be able to assess, select, and implement technology to optimize the client-patient experience, delivery of healthcare, and work-life balance for the veterinary team. Veterinarians must also continue their own education regarding technology by engaging relevant experts and opinion leaders.
Using a Bayesian Network Predictive Model to Understand Vulnerability of Australian Sheep Producers to a Foot and Mouth Disease Outbreak
Authors: Manyweathers Jennifer , Maru Yiheyis , Hayes Lynne , Loechel Barton , Kruger Heleen , Mankad Aditi , Xie Gang , Woodgate Rob , Hernandez-Jover Marta
Published: June 2021

To maintain and strengthen Australia's competitive international advantage in sheep meat and wool markets, the biosecurity systems that support these industries need to be robust and effective. These systems, strengthened by jurisdictional and livestock industry investments, can also be enhanced by a deeper understanding of individual producer risk of exposure to animal diseases and capacity to respond to these risks. This observational study developed a Vulnerability framework, built from current data from Australian sheep producers around behaviors and beliefs that may impact on their likelihood of Exposure and Response Capacity (willingness and ability to respond) to an emergency animal disease (EAD). Using foot and mouth disease (FMD) as a model, a cross-sectional survey gathered information on sheep producers' demographics, and their practices and beliefs around animal health management and biosecurity. Using the Vulnerability framework, a Bayesian Network (BN) model was developed as a first attempt to develop a decision making tool to inform risk based surveillance resource allocation. Populated by the data from 448 completed questionnaires, the BN model was analyzed to investigate relationships between variables and develop producer Vulnerability profiles. Respondents reported high levels of implementation of biosecurity practices that impact the likelihood of exposure to an EAD, such as the use of appropriate animal movement documentation (75.4%) and isolation of incoming stock (64.9%). However, adoption of other practices relating to feral animal control and biosecurity protocols for visitors were limited. Respondents reported a high uptake of Response Capacity practices, including identifying themselves as responsible for observing (94.6%), reporting unusual signs of disease in their animals (91.0%) and daily/weekly inspection of animals (90.0%). The BN analysis identified six Vulnerability typologies, with three levels of Exposure (high, moderate, low) and two levels of Response Capacity (high, low), as described by producer demographics and practices. The most influential Exposure variables on producer Vulnerability included adoption levels of visitor biosecurity and visitor access protocols. Findings from this study can guide decisions around resource allocation to improve Australia's readiness for EAD incursion and strengthen the country's biosecurity system.
Vertebrobasilar Contribution to Cerebral Arterial System of Dromedary Camels (Camelus dromedarius)
Authors: Al Aiyan Ahmad , Menon Preetha , AlDarwich Adnan , Qablan Moneeb , Hammoud Maha , Shawaf Turke , Richardson Ken
Published: June 2021

It is hypothesized that in the “more highly evolved” mammals, including the domesticated mammals, that the brainstem and the cerebellum receive arterial blood through the vertebrobasilar system whilst the internal carotid arteries primarily supply the forebrain. In camels, the arterial blood supply to the brain differs from that of ruminants since the internal carotid artery and the rostral epidural rete mirabile (RERM) are both present and the basilar artery contributes a significant proportion of cerebral afferent blood. In this study, we described the anatomical distribution of the vertebrobasilar system arterial supply in the dromedary. Secondly, we determined the direction of blood flow within the vertebral and basilar arteries using transcranial color doppler ultrasonography. Thirdly, we quantified the percentage arterial contributions of the carotid and vertebrobasilar systems to the dromedary brain. Fifty-five heads of freshly slaughtered male Omani dromedaries aged 2–6 years were dissected to determine the distribution and topography of the arterial distribution to the brain. Their anatomical orientation was assessed by casting techniques using epoxy resin, polyurethane resin and latex neoprene. The epoxy resin and polyurethane resin casts of the head and neck arteries were used to measure the diameter of vertebrobasilar arterial system and carotid arterial system at pre-determined locations. These arterial diameters were used to calculate the percentage of blood supplied by each arterial system. The vertebrobasilar system in dromedary camels consists of paired vertebral arteries that contribute to the ventral spinal artery and basilar artery at multiple locations. In most specimens the vertebral artery was the primary contributor to the basilar artery compared to that of the ventral spinal artery. In four specimens the ventral spinal arteries appear to be the dominant contributor to the basilar artery. Transcranial color doppler ultrasonography confirmed that the direction of blood flow within the vertebral and basilar arteries was toward the brain in animals examined in ventral recumbency and when standing. The vertebrobasilar system contributes 34% of the blood supply to the brain. The vertebrobasilar system is the exclusive supply to the medulla oblongata, pons and cerebellum.
Movement Analysis to Associate Broiler Walking Ability with Gait Scoring
Authors: Pereira Danilo Florentino , Nääs Irenilza de Alencar , Lima Nilsa Duarte da Silva
Published: June 2021

The genetic development of the commercial broiler has led to body misconfiguration and consequent walking disabilities, mainly at the slaughter age. The present study aimed to identify broiler locomotion ability using image analysis automatically. A total of 40 broilers that were 40 d old (male and female) were placed to walk on a specially built runway, and their locomotion was recorded. An image segmentation algorithm was developed, and the coordinates of the bird’s center of mass were extracted from the segmented images for each frame analyzed, and the unrest index (UI) was applied. We calculated the center of mass’s movement of the broiler walking lateral images capturing the bird’s displacement speed in the onward direction. Results indicated that broiler walking speed on the runway tends to decrease with the increase of the gait score. The locomotion did not differ between males or females. The proposed algorithm was efficient in predicting the broiler gait score based on their displacement speed.
Foliar and Root Applications of Vegetal-Derived Protein Hydrolysates Differentially Enhance the Yield and Qualitative Attributes of Two Lettuce Cultivars Grown in Floating System
Authors: Cristofano Francesco , El-Nakhel Christophe , Pannico Antonio , Giordano Maria , Colla Giuseppe , Rouphael Youssef
Published: June 2021

Lettuce (Lactuca sativa L.) is a leafy vegetable cultivated widely for its fast and year-round production and its beneficial phytochemical content, which may be boosted further by plant biostimulants that are considered eco-sustainable means for enhancing horticultural crop production. A greenhouse experiment was carried out to evaluate the yield and qualitative parameters of two differently pigmented lettuce cultivars grown in a floating raft system either untreated or treated (leaf, root or leaf/root application) with vegetal protein hydrolysates (PHs). For foliar application (F), lettuce plants were sprayed at a dose of 3 mL L−1, whereas for root application, 0.15 (T1) or 0.3 (T2) mL L−1 was applied to the nutrient solution alone or in combination with foliar spray (T1 + F and T2 + F) with the same foliar concentration. Bio-morphometric and production data were collected after harvest. Physiological and plant nutrition assays included leaf gas exchange, leaf fluorescence, SPAD index, mineral content, carotenoids, total phenols, total ascorbic acid content and antioxidant activities. Cultivar-specific reactions to biostimulant application were noted: whilst the green pigmented cultivar thrived under nutrient solution applications and recorded higher yield by 82.7% (T1) or (T1 + F) and 71.7% (T2), the red cultivar thrived under combined treatments, yielding 55.4% (T2 + F) higher than control and providing the most concentrated phytochemical content. These latter treatments also engendered the highest SPAD index, Fv/Fm ratio, CO2 assimilation, stomatal conductance and transpiration. In addition, the T2 + F treatment boosted ‘Canasta’ hydrophilic antioxidant activity (21.9%) and total ascorbic acid (5.6-fold). Nutrient solution treatments alone proved advantageous when compared to foliar treatments, while mixed treatments proved genotype-specific. New research on genotype specificity of biostimulant effects is warranted for future use, in order to rationalize biostimulant application modes and dosages.
Objective Method for Determining the Importance of Unprecedented Restlessness as a Rice Crisis Indicator at the National Level
Authors: Hidayat Yuyun , Purwandari Titi , Ratnasari Dewi , Sukono  , Saputra Jumadil , Subiyanto
Published: June 2021

In this study, we use restlessness as an input for a rice crisis indicator, since restlessness rather than rice price provides a comparable year-to year context. We outline the significant increase in the use of unprecedented restlessness (UR) as an indicator for rice crises. The UR approach involves a precedence analysis, in contrast with the existing approach, the price shock analysis. We test UR as a new indicator for rice crises at the national level, which can be applied in Asia and other countries around the world where rice is the staple food. Strong indicators point out the effectiveness of strategic government programs and are able to assess solutions and detect rice crises, while weak indicators are only reliable in detecting whether or not there has been a crisis. UR is tested across 43 countries using two new statistics: success probability (SP) and constraint probability (CP). As a consequence of SP and CP calculations, a large number of IMR control charts for UR analysis are constructed to provide evidence that UR is a strong indicator. The optimum validity measurement result is achieved with SP = 8/26 = 0.31 and CP = 8/14 = 0.57. This means that the UR detects and is followed by only 31% of riot events. Since the value of SP is less than 0.6, we can conclude that the UR indicator is not considered valid as an indicator of rice crises at the national level. The values of CP and SP are determined subjectively as equal to 0.6. This is the main cause of the emergence of new problems in the calibration of UR as an indicator of rice crises. The subjective success criteria trigger a question regarding why the value is 0.6, for which there is no scientific justification. Based on this background, we continue to objectively establish success criteria for UR validity. After conducting a risk analysis involving a crisis recovery cost (CRC) to crisis anticipation cost (CAC) ratio, it is found that the probability of the CRC-to-CAC ratio having values greater than 7 is 0.76, which means the CRC-to-CAC ratio tends to be higher than 7. Objectively, it is concluded that UR, which has been defined as rice crisis indicator at the national level, is an important indicator.
Tomato Graft Union Failure Is Associated with Alterations in Tissue Development and the Onset of Cell Wall Defense Responses
Authors: Frey Carlos , Álvarez Rafael , Encina Antonio , Acebes José Luis
Published: June 2021

Grafting is a technique applied to a considerable number of crops, with tomato standing out. However, this technique is limited by the obtaining of unfunctional grafts, which decrease the success rate and therefore the benefits achieved. The aim of this work was to analyze the failure in intraspecific grafting of tomato plants, focusing on tissue development, cell wall defense reactions, and the distribution of starch and soluble sugars at the graft junction. The success rate in autografts was higher than that of homografts and heterografts. Unfunctional homografts and heterografts showed similar responses: absence of vascular reconnections and lack of adhesion between scion and rootstock, even though callus cell clusters and differentiation of new vasculature were produced. The scions of unfunctional grafts accumulated more starch and soluble sugars than the rootstocks, showing a strong asymmetry in the response. In addition, three types of deposits were observed in the cell walls of unfunctional grafts: lignin, suberin, and callose, with the combined accumulation of more than one of them being frequent, particularly lignin and suberin. These deposits apparently prevent adhesion and seem to be a major cause of graft failure.
Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost
Authors: Han Hui , Bai Mohan , Chen Yanting , Gong Yali , Wu Ming , Yang Hefa , Chen Qing , Xu Ting , Wei Yuquan , Ding Guochun , Li Ji
Published: June 2021

Although composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes (sul1 and sul2) in soil fertilized by compost spiked with two concentrations of sulfadiazine (1 and 10 mg kg−1) were studied intensively by qPCR and high throughput sequencing based on a two-month microcosm experiment. The concentration of sulfadiazine decreased rapidly after spiking from 25% at Day 1 to less than 2.7% at Day 60. Relative abundance of both sul1 and sul2 were significantly higher in soil amended with compost than the non-amended control at Day 1 and slightly decreased with incubation time except for sul2 in the S10 treatment. Soil bacterial communities were transiently shifted by compost fertilization regardless of the presence of sulfadiazine. Relative abundance of genera in three hubs positively interlinked with sul1 and sul2 were significantly higher in compost treated soil than the control at Day 1, 7 and 21, but not at Day 60. High throughput sequencing analyses revealed that most detected (>67% in relative abundance) sul1 and sul2 genotypes sharing >99% similarity with those found in gammaproteobacterial pathogens frequently were commonly present in compost and soil. These results indicated that compost fertilization might increase the abundance rather than diversity of sulfadiazine-resistant populations in soil, which may be facilitated by the presence of sulfadiazine.