bluuebunny/crossref_metadata_2025 huggingface dataset:
Nurses’ Adherence to Patient Safety Principles: A Systematic Review
Authors: Vaismoradi Mojtaba , Tella Susanna , A. Logan Patricia , Khakurel Jayden , Vizcaya-Moreno Flores
Published: March 2020

Background: Quality-of-care improvement and prevention of practice errors is dependent on nurses’ adherence to the principles of patient safety. Aims: This paper aims to provide a systematic review of the international literature, to synthesise knowledge and explore factors that influence nurses’ adherence to patient-safety principles. Methods: Electronic databases in English, Norwegian, and Finnish languages were searched, using appropriate keywords to retrieve empirical articles published from 2010–2019. Using the theoretical domains of the Vincent’s framework for analysing risk and safety in clinical practice, we synthesized our findings according to ‘patient’, ‘healthcare provider’, ‘task’, ‘work environment’, and ‘organisation and management’. Findings: Six articles were found that focused on adherence to patient-safety principles during clinical nursing interventions. They focused on the management of peripheral venous catheters, surgical hand rubbing instructions, double-checking policies of medicines management, nursing handover between wards, cardiac monitoring and surveillance, and care-associated infection precautions. Patients’ participation, healthcare providers’ knowledge and attitudes, collaboration by nurses, appropriate equipment and electronic systems, education and regular feedback, and standardization of the care process influenced nurses’ adherence to patient-safety principles. Conclusions: The revelation of individual and systemic factors has implications for nursing care practice, as both influence adherence to patient-safety principles. More studies using qualitative and quantitative methods are required to enhance our knowledge of measures needed to improve nurse’ adherence to patient-safety principles and their effects on patient-safety outcomes.
A Cross-Level Investigation of Team-Member Exchange on Team and Individual Job Crafting with the Moderating Effect of Regulatory Focus
Authors: Hung Tsang-Kai , Wang Chih-Hung , Tian Mu , Yang Ya-Jiun
Published: March 2020

Within the framework of regulatory focus theory, this study examines the issues of job crafting. This study adopts purposive sampling as a means to collect data. A total of 123 teams with 514 members were invited to participate in the survey, and 91 teams with 354 members provided valid questionnaire responses for data analysis. Mplus 7.0 was applied to conduct data analysis and verification. Data analysis demonstrates that (1) team-member exchange (TMX) exerts a positive influence on team job crafting and individual job crafting; (2) team job crafting positively affects individual job crafting; (3) TMX can positively affect individual job crafting via team job crafting; and (4) a prevention focus has a moderated mediation effect on the indirect relationship between TMX and individual job crafting. Based on its findings, this study has both practical and theoretical implications. Academically, it can be regarded as a pioneering academic endeavor. Practically, this study can enhance teamwork, postulate job flow, and promote the quality of member relationships, thus boosting individual job crafting performance.
MicroRNA (miRNA): A New Dimension in the Pathogenesis of Antiphospholipid Syndrome (APS)
Authors: Kotyla Przemysław J. , Islam Md Asiful
Published: March 2020

MicroRNAs (miRNAs) are single-stranded, endogenous RNA molecules that play a significant role in the regulation of gene expression as well as cell development, differentiation, and function. Recent data suggest that these small molecules are responsible for the regulation of immune responses. Therefore, they may act as potent modulators of the immune system and play an important role in the development of several autoimmune diseases. Antiphospholipid syndrome (APS) is an autoimmune systemic disease characterized by venous and/or arterial thromboses and/or recurrent fetal losses in the presence of antiphospholipid antibodies (aPLs). Several lines of evidence suggest that like other autoimmune disorders, miRNAs are deeply involved in the pathogenesis of APS, interacting with the function of innate and adaptive immune responses. In this review, we characterize miRNAs in the light of having a functional role in the immune system and autoimmune responses focusing on APS. In addition, we also discuss miRNAs as potential biomarkers and target molecules in treating APS.
Mechanisms Responsible for the Anticoagulant Properties of Neurotoxic Dendroaspis Venoms: A Viscoelastic Analysis
Authors: Nielsen Vance G. , Wagner Michael T. , Frank Nathaniel
Published: March 2020

Using thrombelastography to gain mechanistic insights, recent investigations have identified enzymes and compounds in Naja and Crotalus species’ neurotoxic venoms that are anticoagulant in nature. The neurotoxic venoms of the four extant species of Dendroaspis (the Black and green mambas) were noted to be anticoagulant in nature in human blood, but the mechanisms underlying these observations have never been explored. The venom proteomes of these venoms are unique, primarily composed of three finger toxins (3-FTx), Kunitz-type serine protease inhibitors (Kunitz-type SPI) and <7% metalloproteinases. The anticoagulant potency of the four mamba venoms available were determined in human plasma via thrombelastography; vulnerability to inhibition of anticoagulant activity to ethylenediaminetetraacetic acid (EDTA) was assessed, and inhibition of anticoagulant activity after exposure to a ruthenium (Ru)-based carbon monoxide releasing molecule (CORM-2) was quantified. Black mamba venom was the least potent by more than two orders of magnitude compared to the green mamba venoms tested; further, Black Mamba venom anticoagulant activity was not inhibited by either EDTA or CORM-2. In contrast, the anticoagulant activities of the green mamba venoms were all inhibited by EDTA to a greater or lesser extent, and all had anticoagulation inhibited with CORM-2. Critically, CORM-2-mediated inhibition was independent of carbon monoxide release, but was dependent on a putative Ru-based species formed from CORM-2. In conclusion, there was great species-specific variation in potency and mechanism(s) responsible for the anticoagulant activity of Dendroaspis venom, with perhaps all three protein classes—3-FTx, Kunitz-type SPI and metalloproteinases—playing a role in the venoms characterized.
Adrenoceptor Expression during Intervertebral Disc Degeneration
Authors: Kupka Johannes , Kohler Annika , El Bagdadi Karima , Bostelmann Richard , Brenneis Marco , Fleege Christoph , Chan Danny , Zaucke Frank , Meurer Andrea , Rickert Marcus , Jenei-Lanzl Zsuzsa
Published: March 2020

Healthy and degenerating intervertebral discs (IVDs) are innervated by sympathetic nerves, however, adrenoceptor (AR) expression and functionality have never been investigated systematically. Therefore, AR gene expression was analyzed in both tissue and isolated cells from degenerated human IVDs. Furthermore, human IVD samples and spine sections of wildtype mice (WT) and of a mouse line that develops spontaneous IVD degeneration (IVDD, in SM/J mice) were stained for ARs and extracellular matrix (ECM) components. In IVD homogenates and cells α1a-, α1b-, α2a-, α2b-, α2c-, β1-, and β2-AR genes were expressed. In human sections, β2-AR was detectable, and its localization parallels with ECM alterations. Similarly, in IVDs of WT mice, only β2-AR was expressed, and in IVDs of SM/J mice, β2AR expression was stronger accompanied by increased collagen II, collagen XII, decorin as well as decreased cartilage oligomeric matrix protein expression. In addition, norepinephrine stimulation of isolated human IVD cells induced intracellular signaling via ERK1/2 and PKA. For the first time, the existence and functionality of ARs were demonstrated in IVD tissue samples, suggesting that the sympathicus might play a role in IVDD. Further studies will address relevant cellular mechanisms and thereby help to develop novel therapeutic options for IVDD.
Molecular and Potential Regulatory Mechanisms of Melanin Synthesis in Harmonia axyridis
Authors: Xiao Da , Chen Xu , Tian Renbin , Wu Mengmeng , Zhang Fan , Zang Liansheng , Harwood James D. , Wang Su
Published: March 2020

Melanization is a common phenomenon in insects, and melanin synthesis is a conserved physiological process that occurs in epidermal cells. Moreover, a comprehensive understanding of the mechanisms of melanin synthesis influencing insect pigmentation are well-suited for investigating phenotype variation. The Asian multi-colored (Harlequin) ladybird beetle, Harmonia axyridis, exhibits intraspecific polymorphism based on relative levels of melanization. However, the specific characteristics of melanin synthesis in H. axyridis remains elusive. In this study, we performed gene-silencing analysis of the pivotal inverting enzyme, tyrosine hydroxylase (TH), and DOPA decarboxylase (DDC) in the tyrosine metabolism pathway to investigate the molecular and regulatory mechanism of melanin synthesis in H. axyridis. Using RNAi of TH and DDC genes in fourth instar larvae, we demonstrated that dopamine melanin was the primary contributor to the overall body melanization of H. axyridis. Furthermore, our study provides the first conclusive evidence that dopamine serves as a melanin precursor for synthesis in the early pupal stage. According to transcription factor Pannier, which is essential for the formation of melanic color on the elytra in H. axyridis, we further demonstrated that suppression of HaPnr can significantly decrease expression levels of HaTH and HaDDC. These results in their entirety lead to the conclusion that transcription factor Pannier can regulate dopamine melanin synthesis in the dorsal elytral epidermis of H. axyridis.
The Pervasive Role of the miR-181 Family in Development, Neurodegeneration, and Cancer
Authors: Indrieri Alessia , Carrella Sabrina , Carotenuto Pietro , Banfi Sandro , Franco Brunella
Published: March 2020

MicroRNAs (miRNAs) are small noncoding RNAs playing a fundamental role in the regulation of gene expression. Evidence accumulating in the past decades indicate that they are capable of simultaneously modulating diverse signaling pathways involved in a variety of pathophysiological processes. In the present review, we provide a comprehensive overview of the function of a highly conserved group of miRNAs, the miR-181 family, both in physiological as well as in pathological conditions. We summarize a large body of studies highlighting a role for this miRNA family in the regulation of key biological processes such as embryonic development, cell proliferation, apoptosis, autophagy, mitochondrial function, and immune response. Importantly, members of this family have been involved in many pathological processes underlying the most common neurodegenerative disorders as well as different solid tumors and hematological malignancies. The relevance of this miRNA family in the pathogenesis of these disorders and their possible influence on the severity of their manifestations will be discussed. A better understanding of the miR-181 family in pathological conditions may open new therapeutic avenues for devasting disorders such as neurodegenerative diseases and cancer.
Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells
Authors: Chiaino Elda , Micucci Matteo , Durante Miriam , Budriesi Roberta , Gotti Roberto , Marzetti Carla , Chiarini Alberto , Frosini Maria
Published: March 2020

The research for innovative treatments against colon adenocarcinomas is still a great challenge. Acacia catechu Willd. heartwood extract (AC) has health-promoting qualities, especially at the gastrointestinal level. This study characterized AC for its catechins content and investigated the apoptosis-enhancing effect in human colorectal adenocarcinoma HT-29 cells, along with its ability to spare healthy tissue. MTT assay was used to describe the time course, concentration dependence and reversibility of AC-mediated cytotoxicity. Cell cycle analysis and AV-PI and DAPI-staining were performed to evaluate apoptosis, together with ROS formation, mitochondrial membrane potential (MMP) changes and caspase activities. Rat ileum and colon rings were tested for their viability and functionality to explore AC effects on healthy tissue. Quantitative analysis highlighted that AC was rich in (±)-catechin (31.5 ± 0.82 mg/g) and (−)-epicatechin (12.5 ± 0.42 mg/g). AC irreversibly decreased cell viability in a concentration-dependent, but not time-dependent fashion. Cytotoxicity was accompanied by increases in apoptotic cells and ROS, a reduction in MMP and increases in caspase-9 and 3 activities. AC did not affect rat ileum and colon rings’ viability and functionality, suggesting a safe profile toward healthy tissue. The present findings outline the potential of AC for colon cancer treatment.
Genetic Transformation of Tribonema minus, a Eukaryotic Filamentous Oleaginous Yellow-Green Alga
Authors: Zhang Yan , Wang Hui , Yang Ruigang , Wang Lihao , Yang Guanpin , Liu Tianzhong
Published: March 2020

Eukaryotic filamentous yellow-green algae from the Tribonema genus are considered to be excellent candidates for biofuels and value-added products, owing to their ability to grow under autotrophic, mixotrophic, and heterotrophic conditions and synthesize large amounts of fatty acids, especially unsaturated fatty acids. To elucidate the molecular mechanism of fatty acids and/or establish the organism as a model strain, the development of genetic methods is important. Towards this goal, here, we constructed a genetic transformation method to introduce exogenous genes for the first time into the eukaryotic filamentous alga Tribonema minus via particle bombardment. In this study, we constructed pSimple-tub-eGFP and pEASY-tub-nptⅡ plasmids in which the green fluorescence protein (eGFP) gene and the neomycin phosphotransferase Ⅱ-encoding G418-resistant gene (nptⅡ) were flanked by the T. minus-derived tubulin gene (tub) promoter and terminator, respectively. The two plasmids were introduced into T. minus cells through particle-gun bombardment under various test conditions. By combining agar and liquid selecting methods to exclude the pseudotransformants under long-term antibiotic treatment, plasmids pSimple-tub-eGFP and pEASY-tub- nptⅡ were successfully transformed into the genome of T. minus, which was verified using green fluorescence detection and the polymerase chain reaction, respectively. These results suggest new possibilities for efficient genetic engineering of T. minus for future genetic improvement.
Targeted Deletion of PTEN in Kisspeptin Cells Results in Brain Region- and Sex-Specific Effects on Kisspeptin Expression and Gonadotropin Release
Authors: Negrón Ariel L. , Yu Guiqin , Boehm Ulrich , Acosta-Martínez Maricedes
Published: March 2020

Kisspeptin-expressing neurons in the anteroventral periventricular nucleus (AVPV) and the arcuate nucleus (ARC) of the hypothalamus relay hormonal and metabolic information to gonadotropin-releasing hormone neurons, which in turn regulate pituitary and gonadal function. Phosphatase and tensin homolog (PTEN) blocks phosphatidylinositol 3-kinase (PI3K), a signaling pathway utilized by peripheral factors to transmit their signals. However, whether PTEN signaling in kisspeptin neurons helps to integrate peripheral hormonal cues to regulate gonadotropin release is unknown. To address this question, we generated mice with a kisspeptin cell-specific deletion of Pten (Kiss-PTEN KO), and first assessed kisspeptin protein expression and gonadotropin release in these animals. Kiss-PTEN KO mice displayed a profound sex and region-specific kisspeptin neuron hyperthrophy. We detected both kisspeptin neuron hyperthrophy as well as increased kisspeptin fiber densities in the AVPV and ARC of Kiss-PTEN KO females and in the ARC of Kiss-PTEN KO males. Moreover, Kiss-PTEN KO mice showed a reduced gonadotropin release in response to gonadectomy. We also found a hyperactivation of mTOR, a downstream PI3K target and central regulator of cell metabolism, in the AVPV and ARC of Kiss-PTEN KO females but not males. Fasting, known to inhibit hypothalamic kisspeptin expression and luteinizing hormone levels, failed to induce these changes in Kiss-PTEN KO females. We conclude that PTEN signaling regulates kisspeptin protein synthesis in both sexes and that its role as a metabolic signaling molecule in kisspeptin neurons is sex-specific.
Non-Photochemical Quenching Involved in the Regulation of Photosynthesis of Rice Leaves under High Nitrogen Conditions
Authors: Cisse Amara , Zhao Xia , Fu Weimeng , Kim Romesh Eric Romy , Chen Tingting , Tao Longxing , Feng Baohua
Published: March 2020

Excess and deficient nitrogen (N) inhibit photosynthesis in the leaves of rice plants, but the underlying mechanism is still unclear. N can improve the chlorophyll content and thus affect photon absorption, but the photosynthetic rate does not increase accordingly. To investigate this mechanism, three concentrations of N treatments were applied to two rice varieties, Zhefu802 and Fgl. The results indicated increased chlorophyll content of leaves with an increased N supply. Little discrepancy was detected in Rubisco enzyme activity and Non-photochemical quenching (NPQ) in the high nitrogen (HN) and moderate nitrogen (MN) treatments. The model that photoinhibition occurs in Zhefu802 due to a lack of balance of light absorption and utilization is supported by the higher malondialdehyde (MDA) content, higher H2O2 content, and photoinhibitory quenching (qI) in HN treatment compared with MN treatment. A lower proportion of N in leaf was used to synthesize chlorophyll for Fgl compared with Zhefu802, reducing the likelihood of photoinhibition under HN treatment. In conclusion, HN supply does not allow ideal photosynthetic rate and increases the likelihood of photoinhibition because it does not sustain the balance of light absorption and utilization. Apart from Rubisco enzyme activity, NPQ mainly contributes to the unbalance. These results of this study will provide reference for the effective N management of rice.
Simple Trans-Platinum Complex Bearing 3-Aminoflavone Ligand Could Be a Useful Drug: Structure-Activity Relationship of Platinum Complex in Comparison with Cisplatin
Authors: Fabijańska Małgorzata , Orzechowska Magdalena , Rybarczyk-Pirek Agnieszka J. , Dominikowska Justyna , Bieńkowska Alicja , Małecki Maciej , Ochocki Justyn
Published: March 2020

Following previous studies devoted to trans–Pt(3-af)2Cl2, in this paper, the molecular structure and intermolecular interactions of the title complex are compared with other cisplatin analogues of which the crystal structures are presented in the Cambridge Structural Database (CSD). Molecular Hirshfeld surface analysis and computational methods were used to examine a possible relationship between the structure and anticancer activity of trans–Pt(3-af)2Cl2. The purpose of the article was also to investigate the effect of hyperthermia on the anticancer activity of cisplatin, cytostatics used in the treatment of patients with ovarian cancer and a new analogue of cisplatin-trans–Pt(3-af)2Cl2. The study was conducted on two cell lines of ovarian cancer sensitive to Caov-3 cytostatics and the OVCAR-3 resistant cisplatin line. The study used the MTT (3-(4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide) cell viability assay, LDH (lactate dehydrogenase), and the quantitative evaluation method for measuring gene expression, i.e., qPCR with TagMan probes. Reduced survivability of OVCAR-3 and Caov-3 cells exposed to cytostatics at elevated temperatures (37 °C, 40 °C, 43 °C) was observed. Hyperthermia may increase the sensitivity of cells to platinum-based antineoplastic drugs and paclitaxel, which may be associated with the reduction of gene expression related to apoptotic processes.
Extracellular Vesicles in Feto–Maternal Crosstalk and Pregnancy Disorders
Authors: Buca Danilo , Bologna Giuseppina , D’Amico Alice , Cugini Sara , Musca Francesca , Febbo Melania , D’Arcangelo Dolores , Buca Davide , Simeone Pasquale , Liberati Marco , Vitacolonna Ester , Miscia Sebastiano , D’Antonio Francesco , Lanuti Paola
Published: March 2020

Extracellular vesicles (EVs) actively participate in inter-cellular crosstalk and have progressively emerged as key players of organized communities of cells within multicellular organisms in health and disease. For these reasons, EVs are attracting the attention of many investigators across different biomedical fields. In this scenario, the possibility to study specific placental-derived EVs in the maternal peripheral blood may open novel perspectives in the development of new early biomarkers for major obstetric pathological conditions. Here we reviewed the involvement of EVs in feto–maternal crosstalk mechanisms, both in physiological and pathological conditions (preeclampsia, fetal growth restriction, preterm labor, gestational diabetes mellitus), also underlining the usefulness of EV characterization in maternal–fetal medicine.
Equivocal Evidence for Colony Level Stress Effects on Bumble Bee Pollination Services
Authors: Greenop Arran , Mica-Hawkyard Nevine , Walkington Sarah , Wilby Andrew , Cook Samantha M , Pywell Richard F , Woodcock Ben A
Published: March 2020

Climate change poses a threat to global food security with extreme heat events causing drought and direct damage to crop plants. However, by altering behavioural or physiological responses of insects, extreme heat events may also affect pollination services on which many crops are dependent. Such effects may potentially be exacerbated by other environmental stresses, such as exposure to widely used agro-chemicals. To determine whether environmental stressors interact to affect pollination services, we carried out field cage experiments on the buff-tailed bumble bee (Bombus terrestris). Using a Bayesian approach, we assessed whether heat stress (colonies maintained at an ambient temperature of 25 °C or 31 °C) and insecticide exposure (5 ng g-1 of the neonicotinoid insecticide clothianidin) could induce behavioural changes that affected pollination of faba bean (Vicia faba). Only the bumble bee colonies and not the plants were exposed to the environmental stress treatments. Bean plants exposed to heat-stressed bumble bee colonies (31 °C) had a lower proportional pod set compared to colonies maintained at 25 °C. There was also weak evidence that heat stressed colonies caused lower total bean weight. Bee exposure to clothianidin was found to have no clear effect on plant yields, either individually or as part of an interaction. We identified no effect of either colony stressor on bumble bee foraging behaviours. Our results suggest that extreme heat stress at the colony level may impact on pollination services. However, as the effect for other key yield parameters was weaker (e.g. bean yields), our results are not conclusive. Overall, our study highlights the need for further research on how environmental stress affects behavioural interactions in plant-pollinator systems that could impact on crop yields.
Effects of Temperature on the Survival and Larval Development of Deiratonotus Japonicus (Brachyura, Camptandriidae) as a Biological Indicator
Authors: Oh Il-Kweun , Lee Seung-Woo
Published: March 2020

Deiratonotus japonicus (D. japonicus) inhabits isolated locations and upstream brackish waters from Kanagawa Prefecture to Okinawa Prefecture in Japan. This species faces the threat of extinction because of changing habitat conditions. Our previous studies have shown that its complete larval development from hatching to metamorphosis consists of five zoeal stages and one megalopal stage. In this study, the effect of temperature on the survival and growth of larval development in D. japonicus under controlled laboratory conditions of 13, 18, 23, 24, 25, and 26 °C was investigated by rearing larvae (30 PSU; 12:12 h light/dark cycle; fed a diet of Brachionus plicatilis rotundiformis and Artemia sp. nauplii). The survival rates and developmental periods were measured for each larval stage. The highest survival rates were obtained at 18–24 °C. Metamorphosis to megalopa occurred at 23–25 °C. There were rapid and synchronous developments at 25–26 °C but delayed and extended developments at 13 °C. The molting period decreased with increasing temperature. With decreasing temperature, the beginning of the development and duration of molting was prolonged. In addition, there were very low survival rates at 13 °C and 26 °C in all zoeal stages. Our results indicate that the early larval stages of D. japonicus are well adapted to 18–24 °C, the range observed in the estuarine marine environment of the Kita River during the breeding season. Optimum larval survival and growth were obtained at 23 °C. Temperature significantly affected the survival rate, developmental period, and molting of the larvae. The relationship between the cumulative periods of development from hatching through individual larval stages (y) and temperatures (T) was described as a power function (y = a × Tb).
Selected Tribological Properties and Vibrations in the Base Resonance Zone of the Polymer Composite Used in the Aviation Industry
Authors: Krzyzak Aneta , Kosicka Ewelina , Borowiec Marek , Szczepaniak Robert
Published: March 2020

The revolution in the global market of composite materials is evidenced by their increasing use in such segments as the transport, aviation, and wind industries. The innovative aspect of this research is the methodology approach, based on the simultaneous analysis of mechanical and tribological loads of composite materials, which are intended for practical use in the construction of aviation parts. Simultaneously, the methodology allows the composition of the composites used in aviation to be optimized. Therefore, the presented tests show the undefined properties of the new material, which are necessary for verification at the application stage. They are also a starting point for further research planned by the authors related to the improvement of the tribological properties of this material. In this article, the selected mechanical and tribological properties of an aviation polymer composite are investigated with the matrix of L285-cured hardener H286 and six reinforcement layers of carbon fabric GG 280P/T. The structure of a polymer composite has a significant influence on its mechanical properties; thus, a tribological analysis in the context of abrasive wear in reciprocating the movement for the specified polymer composite was performed. Moreover, the research was expanded to dynamic analysis for the discussed composite. This is crucial knowledge of material dynamics in the context of aviation design for the conditions of resonance vibrations. For this reason, experimental dynamical investigations were performed to determine the basic resonance of the material and its dynamics behavior response. The research confirmed the assumed hypotheses related to the abrasive wear process for the newly developed material, as well as reporting an empirical evaluation of the dependencies of the resonance zone from the fabric orientation sets.
Synthesis and Characterization of Bismuth-Cerium Oxides for the Catalytic Oxidation of Diesel Soot
Authors: Hebert Sabrina C. , Stöwe Klaus
Published: March 2020

In this paper, the syntheses of a set of cerium-bismuth mixed oxides with the formula Ce1−xBixO2−x/2, where the range of x is 0.0 to 1.0 in 10 mol% steps, via co-precipitation methods is described. Two synthesis routes are tested: The “normal” and the so called “reverse strike” (RS) co-precipitation route. The syntheses are performed with an automated synthesis robot. The activity for Diesel soot oxidation is measured by temperature programmed oxidation with an automated, serial thermogravimetric and differential scanning calorimetry system (TGA/DSC). P90 is used as a model soot. An automated and reproducible tight contact between soot and catalyst is used. The synthesized catalysts are characterized in terms of the specific surface area according to Brunauer, Emmett and Teller (SBET), as well as the dynamic oxygen storage capacity (OSCdyn). The crystalline phases of the catalysts are analysed by powder X-ray diffraction (PXRD) and Raman spectroscopy. The elemental mass fraction of the synthesized catalysts is verified by X-ray fluorescence (XRF) analysis. A correlation between the T50 values, OSCdyn and SBET has been discovered. The best catalytic performance is exhibited by the catalyst with the formula RS-Ce0.8Bi0.2Ox which is synthesized by the reverse strike co-precipitation route. Here, a correlation between activity, OSCdyn, and SBET can be confirmed based on structural properties.
The Critical Raw Materials in Cutting Tools for Machining Applications: A Review
Authors: Rizzo Antonella , Goel Saurav , Luisa Grilli Maria , Iglesias Roberto , Jaworska Lucyna , Lapkovskis Vjaceslavs , Novak Pavel , Postolnyi Bogdan O. , Valerini Daniele
Published: March 2020

A variety of cutting tool materials are used for the contact mode mechanical machining of components under extreme conditions of stress, temperature and/or corrosion, including operations such as drilling, milling turning and so on. These demanding conditions impose a seriously high strain rate (an order of magnitude higher than forming), and this limits the useful life of cutting tools, especially single-point cutting tools. Tungsten carbide is the most popularly used cutting tool material, and unfortunately its main ingredients of W and Co are at high risk in terms of material supply and are listed among critical raw materials (CRMs) for EU, for which sustainable use should be addressed. This paper highlights the evolution and the trend of use of CRMs) in cutting tools for mechanical machining through a timely review. The focus of this review and its motivation was driven by the four following themes: (i) the discussion of newly emerging hybrid machining processes offering performance enhancements and longevity in terms of tool life (laser and cryogenic incorporation); (ii) the development and synthesis of new CRM substitutes to minimise the use of tungsten; (iii) the improvement of the recycling of worn tools; and (iv) the accelerated use of modelling and simulation to design long-lasting tools in the Industry-4.0 framework, circular economy and cyber secure manufacturing. It may be noted that the scope of this paper is not to represent a completely exhaustive document concerning cutting tools for mechanical processing, but to raise awareness and pave the way for innovative thinking on the use of critical materials in mechanical processing tools with the aim of developing smart, timely control strategies and mitigation measures to suppress the use of CRMs.
Numerical Modelling of Concrete-to-UHPC Bond Strength
Authors: Valikhani Alireza , Jaberi Jahromi Azadeh , Mantawy Islam M. , Azizinamini Atorod
Published: March 2020

Ultra-High Performance Concrete (UHPC) has been a material of interest for retrofitting reinforced concrete elements because of its pioneer mechanical and material properties. Numerous experimental studies for retrofitting concrete structures have shown an improvement in durability performance and structural behaviour. However, conservative and sometimes erroneous estimates for bond strength are used for numerically calculating the strength of the composite members. In addition, different roughening methods have been used to improve the bond mechanism; however, there is a lack of numerical simulation for the force transfer mechanism between the concrete substrate and UHPC as a repair material. This paper presents an experimental and numerical programme designed to characterize the interfacial properties of concrete substrate and its effect on the bond strength between the two materials. The experimental programme evaluates the bond strength between the concrete substrates and UHPC with two different surface preparations while using bi-surface test and additional material tests, including cylinder and cube tests for compression property, direct tension test, and flexural test to complement UHPC tensile properties. Non-linear finite element analysis was conducted, which uses a numerical zero thickness volume model to define the interface bond instead of a traditional fixed contact model. The numerical results from the zero thickness volume model show good agreement with the experimental results with a reduction in error by 181% and 24% for smooth and rough interface surfaces if compared to the results from the model with a fixed contact.
Artificial Neural Network and Response Surface Methodology Based Analysis on Solid Particle Erosion Behavior of Polymer Matrix Composites
Authors: Antil Sundeep Kumar , Antil Parvesh , Singh Sarbjit , Kumar Anil , Pruncu Catalin Iulian
Published: March 2020

Polymer-based fibrous composites are gaining popularity in marine and sports industries because of their prominent features like easy to process, better strength to weight ratio, durability and cost-effectiveness. Still, erosive behavior of composites under cyclic abrasive impact is a significant concern for the research fraternity. In this paper, the S type woven glass fibers reinforced polymer matrix composites (PMCs) are used to analyze the bonding behavior of reinforcement and matrix against the natural abrasive slurry. The response surface methodology is adopted to analyze the effect of various erosion parameters on the erosion resistance. The slurry pressure, impingement angle and nozzle diameter, were used as erosion parameters whereas erosion loss, i.e., weight loss during an erosion phenomenon was considered as a response parameter. The artificial neural network model was used to validate the attained outcomes for an optimum solution. The comparative analysis of response surface methodology (RSM) and artificial neural network (ANN) models shows good agreement with the erosion behavior of glass fiber reinforced polymer matrix composites.
Multiple Transitions in Permalloy Half-Ring Wires with Finite-Size Effect
Authors: Wu Cheng-Yi , Yen Shiow-Kang
Published: March 2020

Six permalloy (Py) half-rings with finite-size from 120 nm to 360 nm were connected in series on five corners. The magnetization reversal processes were investigated by the measurement of anisotropic magnetoresistance (AMR). The number of switching jumps in the AMR loops, from zero to five, varied with the longitudinal applied field. These discrete jumps resulted from domain wall (DW) nucleating and depinning on the corners. The larger external field had a fewer number of jumps in the magnetoresistance (MR) curve. This reproducible and particular response of the domain wall device in the half-ring wires pattern might be one of the new promising magnetoelectronic devices.
Potato Starch Utilization in Ecological Loose-Fill Packaging Materials—Sustainability and Characterization
Authors: Combrzyński Maciej , Matwijczuk Arkadiusz , Wójtowicz Agnieszka , Oniszczuk Tomasz , Karcz Dariusz , Szponar Jarosław , Niemczynowicz Agnieszka , Bober Dariusz , Mitrus Marcin , Kupryaniuk Karol , Stasiak Mateusz , Dobrzański Bohdan , Oniszczuk Anna
Published: March 2020

Biodegradable materials are used in the manufacture of packaging and compostable films and various types of medical products. These have demonstrated high potential in medical applications: cardiac, vascular and orthopaedic conditions in adults as well in children. In our research, the extrusion-cooking technique was used to obtain environmentally friendly loose-fill foams as packaging. Potato starch was the basic raw material. Polyvinyl alcohol was used as an additive in the amount of 1%, 2% and 3% to replace starch. The components were mixed and moistened with water to various initial moisture contents of the blend (17%, 18% and 19%). The processing of starch foams employed the TS-45 single screw extruder-cooker (Gliwice, Poland) with the L/D ratio of 12. The foams were processed with various screw speeds (100 and 130 rpm) and with two types of forming dies (circular and ring die). The extrusion-cooking process efficiency (kg h−1) and the energy consumption (kWh kg−1) during the processing were also measured. The results showed that the processing efficiency of potato starch foams varied depending on the level of polyvinyl alcohol, the shape of the forming die and the screw speed applied. The analysis of energy consumption, mechanical properties and FTIR analyses demonstrated that the type of the forming die and the initial moisture level had the most significant impact on specific energy demands during the processing of potato starch-based foams.
Simulation Study of Adhesive Material for Sandwich Panel under Edgewise Compression Condition
Authors: Jiang Lanxin , Yang Bing , Xiao Shoune , Yang Guangwu , Zhu Tao , Dong Dawei
Published: March 2020

In order to study the interfacial adhesive material simulation method of a sandwich structure with aluminum alloy panels and a low-density foam core under edgewise compression condition, two finite element models were defined using material model no. 185 (MAT 185) adhesive element and tiebreak contact, respectively, by LS-DYNA. Under the conditions of different loading rates, and element sizes, the effects of peak load, energy absorption, failure mode of adhesive layer and the influence degree of the changing condition on the calculated results were compared between the two models, and then compared with the experiment results and theoretical results. The higher the loading rate was, or the smaller the element size was, the higher the peak load was. The simulation results obtained using MAT 185 were closer to the experimental results under the edgewise compression condition.
Investigations into the Improvement of the Mechanical Properties of Ti-5Al-4Mo-4Cr-2Sn-2Zr Titanium Alloy by Using Low Energy Laser Peening without Coating
Authors: Xue Dingyuan , Jiao Yang , He Weifeng , Shen Xiaojun , Gao Yangjun , Wang Lili
Published: March 2020

Mechanical properties, such as residual stress, micro-hardness and fatigue performance, of the Ti-5Al-4Mo-4Cr-2Sn-2Zr titanium alloy were improved via the laser peening without coating (LPwC) with a water-penetrable wavelength of 532 nm and pulse duration of 10 ns. In this paper, three kinds of laser energy, namely 85, 110 and 160 mJ were used to process the samples. The titanium alloy samples were also peened with different impact times (1, 3 or 5 impacts) at the energy of 85 mJ. The micro-hardness and residual stress distribution results provided that LPwC can introduce compressive residual stress (CRS) and also induce hardening of the target materials. Further, micro-hardness and CRS showed the increasing trends when the laser impact times increased. However, the CRS and micro-hardness decreased while the laser energy increased from 110 to 160 mJ, which was attributed to the dynamic equilibrium between the thermal and mechanical effects of LPwC. High cycle fatigue strength of the titanium alloy was significantly improved from 360 to 490.3 MPa after three impacts LPwC. The strengthening mechanism of fatigue strength subjected to LPwC was a combined effect between the laser-induced CRS and the high-density dislocations.
Antimalarial Peptide and Polyketide Natural Products from the Fijian Marine Cyanobacterium Moorea producens
Authors: Sweeney-Jones Anne , Gagaring Kerstin , Antonova-Koch Jenya , Zhou Hongyi , Mojib Nazia , Soapi Katy , Skolnick Jeffrey , McNamara Case , Kubanek Julia
Published: March 2020

A new cyclic peptide, kakeromamide B (1), and previously described cytotoxic cyanobacterial natural products ulongamide A (2), lyngbyabellin A (3), 18E-lyngbyaloside C (4), and lyngbyaloside (5) were identified from an antimalarial extract of the Fijian marine cyanobacterium Moorea producens. Compounds 1 and 2 exhibited moderate activity against Plasmodium falciparum blood-stages with EC50 values of 0.89 and 0.99 µM, respectively, whereas 3 was more potent with an EC50 value of 0.15 nM. Compounds 1, 4, and 5 displayed moderate liver-stage antimalarial activity against P. berghei liver schizonts with EC50 values of 1.1, 0.71, and 0.45 µM, respectively. The threading-based computational method FINDSITEcomb2.0 predicted the binding of 1 and 2 to potentially druggable proteins of Plasmodium falciparum, prompting formulation of hypotheses about possible mechanisms of action. Kakeromamide B (1) was predicted to bind to several Plasmodium actin-like proteins and a sortilin protein suggesting possible interference with parasite invasion of host cells. When 1 was tested in a mammalian actin polymerization assay, it stimulated actin polymerization in a dose-dependent manner, suggesting that 1 does, in fact, interact with actin.
Fucoidans: Downstream Processes and Recent Applications
Authors: Zayed Ahmed , Ulber Roland
Published: March 2020

Fucoidans are multifunctional marine macromolecules that are subjected to numerous and various downstream processes during their production. These processes were considered the most important abiotic factors affecting fucoidan chemical skeletons, quality, physicochemical properties, biological properties and industrial applications. Since a universal protocol for fucoidans production has not been established yet, all the currently used processes were presented and justified. The current article complements our previous articles in the fucoidans field, provides an updated overview regarding the different downstream processes, including pre-treatment, extraction, purification and enzymatic modification processes, and shows the recent non-traditional applications of fucoidans in relation to their characters.
Deciphering the Metabolic Pathways of Pitaya Peel after Postharvest Red Light Irradiation
Authors: Wu Qixian , Gao Huijun , Zhang Zhengke , Li Taotao , Qu Hongxia , Jiang Yueming , Yun Ze
Published: March 2020

Red light irradiation can effectively prolong the shelf-life of many fruit. However, little is known about red light-induced metabolite and enzyme activities. In this study, pitaya fruit was treated with 100 Lux red light for 24 h. Red light irradiation significantly attenuated the variation trend of senescence traits, such as the decrease of total soluble solid (TSS) and TSS/acidity (titratable acidity, TA) ratio, the increase of TA, and respiratory rate. In addition, the reactive oxygen species (ROS) related characters, primary metabolites profiling, and volatile compounds profiling were determined. A total of 71 primary metabolites and 67 volatile compounds were detected and successfully identified by using gas chromatography mass spectrometry (GC-MS). Red light irradiation enhanced glycolysis, tricarboxylic acid (TCA) cycle, aldehydes metabolism, and antioxidant enzymes activities at early stage of postharvest storage, leading to the reduction of H2O2, soluble sugars, organic acids, and C-6 and C-7 aldehydes. At a later stage of postharvest storage, a larger number of resistance-related metabolites and enzyme activities were induced in red light-treated pitaya peel, such as superoxide dismutase (SOD), ascorbate peroxidase (APX), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging, reducing power, fatty acids, and volatile aroma.
Plasmid-Mediated Colistin Resistance (mcr-1) in Escherichia coli from Non-Imported Fresh Vegetables for Human Consumption in Portugal
Authors: Manageiro Vera , Jones-Dias Daniela , Ferreira Eugénia , Caniça Manuela
Published: March 2020

In this study, we report the presence of the plasmid-mediated colistin resistance (PMCR)-encoding gene mcr-1 in an Escherichia coli isolate, INSali25, recovered from lettuce produced and marketed in Portugal. Colistin MIC from the vegetable E. coli isolate—determined by microdilution broth method according to EUCAST guidelines—revealed a non-wild-type phenotype of colistin (MIC 16 mg/L). To understand the genetic background of E. coli INSali25, we performed whole genome sequencing. Plasmid sequencing was also performed after plasmid DNA extraction from the transconjugant TcINSali25 (mcr-1). Directed bioinformatics analysis identified the mcr-1 gene in a 39,998 bp length contig, with an upstream region including the antibiotic resistance gene blaTEM-1 in a partial transposon Tn2, truncated by the insertion sequence IS26 and showing >99% identity with previously described mcr-1-harboring IncHI2 plasmids. Further in silico analysis showed the presence of additional genes conferring resistance to β-lactams (blaTEM-1), aminoglycosides (aadA1, aph(4)-Ia, aph(6)-Id, aac(3)-Iv), macrolides (mdf(A)-type), phenicol (floR-type), tetracycline (tetA), and sulphonamides (sul2). INSali25 isolate belonged to the ST1716 lineage and showed the fimH54 and fumC27 alleles. Lettuce is a vegetable that is commonly consumed fresh and not subjected to any cooking process, which may amplify human food safety risks. Moreover, the occurrence of plasmid-mediated colistin resistance in a sample that was not imported and was acquired in a large retail store reinforces the widespread distribution of mcr-1.
Geochemistry and Origin of Scheelites from the Xiaoyao Tungsten Skarn Deposit in the Jiangnan Tungsten Belt, SE China
Authors: Su Qiangwei , Mao Jingwen , Sun Jia , Zhao Linghao , Xu Shengfa
Published: March 2020

The type, association, variations, and valence states of several metal elements of scheelite can trace the source and evolution of the ore-forming fluids. There are four types of scheelite from the Xiaoyao deposit: (1) scheelite intergrown with garnet in the proximal zone (Sch1a) and with pyroxene in the distal zone (Sch1b), (2) scheelite replaced Sch1a (Sch2a) and crystallized as rims around Sch1b (Sch2b), (3) quartz vein scheelite with oscillatory zoning (Sch3), and 4) scheelite (Sch4) within micro-fractures of Sch3. Substitutions involving Mo and Cd are of particular relevance, and both elements are redox-sensitive and oxidized Sch1a, Sch2b, Sch3 are Mo and Cd enriched, relatively reduced Sch1b, Sch2a, Sch4 are depleted Mo and Cd. Sch1a, Sch2a, Sch3, and Sch4 are characterized by a typical right-inclined rare earth element (REE) pattern, inherited from ore-related granodiorite and modified by the precipitation of skarn minerals. Sch1b and Sch2b are characterized by low light rare earth element/heavy rare earth element (LREE/HREE) ratios, influenced by a shift in fO2 during fluid-rock alteration. Sch1b, Sch2b and Sch3 have higher Sr contents than those of Sch1a and Sch2a, reveal that host-rock alteration and fluid–rock interaction have elevated Sr contents. The Y/Ho ratios of scheelite gradually increase from skarn to quartz vein stages, due to fluid fractionation caused by fluid–rock interaction. Thus, the variation in REE and trace elements in scheelite in time and space reflects a complex magmatic-hydrothermal process involving various fluid–rock interactions and fluid mixing.
The Color Palette of the Mosaics in the Roman Villa of Noheda (Spain)
Authors: Schibille Nadine , Boschetti Cristina , Valero Tévar Miguel Ángel , Veron Emmanuel , de Juan Ares Jorge
Published: March 2020

Excavations at the Roman villa of Noheda (Spain) revealed the remains of an exceptionally elaborate fourth-century floor mosaic that contains a surprisingly large number of glass tesserae, representing a broad spectrum of colors. This paper presents the results of the chemical (LA-ICP-MS) and microstructural analyses (SEM-EDS, XRPD) of 420 glass tesserae from these mosaics. The high number of data allowed us to establish the compositional variability and to elucidate questions of supply in relation to a large-scale artistic campaign. The tesserae from Noheda were almost exclusively made from recycled mixed Roman Mn and Sb base glass, thus demonstrating that recycling of Roman base glasses was common practice in the fourth century, occurring on a near industrial scale. It also suggests that the workshops specializing in the production of mosaic tesserae might have been in the western Mediterranean. A limited number of coloring and opacifying additives (Mn, Co, Cu, Sb, Pb) were identified, which resulted in a wide range of hues. These were differentially associated with various trace elements, which implies the use of different raw materials. A sub-set of red, green, and orange tesserae reflect distinct base glass characteristics as well as coloring technologies that point to an Egyptian provenance.