bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh media edukasi gizi tentang indeks glikemik terhadap kadar glukosa darah pasien diabetes mellitus
Authors: Eliza Eliza , Telisa Imelda , Meilina Ayu , Sumarman Sumarman
Published: September 2024

Background: Diabetes Mellitus (DM) cases are increasing every year, IDF 2021 data shows DM cases aged 20-79 years with a total of 537 million people (10,5%), predicted to continue to increase in 2030 to 643 million people (11,3%) . DM sufferers can basically live a normal life if they follow four pillars, one of which is complying with education. It is important for patients to receive education in choosing foods that contain a low glycemic index because they are more slowly absorbed by the body into glucose, so that glucose levels in the body become more controlled. DM sufferers are advised to consume foods with a low glycemic index because these foods are more slowly absorbed by the body into glucose so that glucose levels in the body are more controlled. The more often DM patients receive education, the better their behavior, especially in choosing foods with a low glycemic index.Objectives: Knowing the effect of providing food glycemic index education on blood glucose levels in type 2 DM patients at Palembang Social Health Center.Methods: The research was carried out at the Palembang Social Health Center for 5 months, namely August to November 2021. Data was collected through measurements, namely body weight and height, interviews in the form of 3x24 hour recall and measurement of blood glucose levels. The type of research used is quantitative research using a Quasi Experimental research design. The research used a pretest and posttest with control group design with 46 respondents. The statistical analysis method used is the Paired T-test. The results of the dependent t-test between the treatment and comparison groups had a p value <0,05 which was equally significant, so it was continued with the independent t-test.Results: The results showed that there was a significant difference in blood glucose levels before and after providing counseling using posters (p=0,002) and booklets (p=0,004).Conclusion: Booklet media had more influence on increasing respondents' knowledge compared to using other media such as posters.
Kualitas pelayanan terhadap kepuasan pasien rawat jalan menggunakan metode servqual di Puskesmas Tawangrejo
Authors: Septyaningrum Intan , Ratnawati Riska , Widiarini Retno
Published: September 2024

Background: Based on the minimum service standard that must be achieved, namely ≥ 90%, however the level of patient satisfaction has not yet reached the Minimum Service Standard (SPM) of 84,98 and there are several complaints from several patients regarding health services, there are still officers who are less friendly and polite to patients and lack of health center staff.Objective: The research aims to analyze the influence of service quality on outpatient satisfaction using the servqual method.Method: This research uses a cross sectional study design. The total sample was 96 respondents with a sampling technique using Proportional Random Sampling. The research location was carried out at the Tawangrejo Community Health Center in 2023. Data collection used a questionnaire and was based on patient visit data. Data processing uses editing, coding and data entry. Analysis used univariate and bivariate analysis with chi-square test using SPSS 16,0Results: The results of data processing using the Chi-Square test show that the p value is 0,040 < α = 0,005, showing a PR value of 2,759 and a value (95% CI = 1,134 – 6,711) which means there is a relationship between service quality and outpatient satisfaction. in the Tawangrejo Community Health Center Working Area.Conclusion: There is an influence between service quality on outpatient satisfaction in the Tawangrejo Community Health Center Working Area. It is hoped that the suggestions given by the Tawangrejo Community Health Center can further maintain and improve the quality of services that have been provided so far and immediately improve patient complaints.KeywordsService Quality, Patient Satisfaction, Outpatient Care
Hubungan hygiene, sanitasi lingkungan dan asupan protein dengan infeksi kecacingan pada balita usia 24-59 bulan di Kabupaten Aceh Tengah
Authors: Ilmi Vanessa Yudha Azizul , Syauqy Ahmad , Noer Etika Ratna
Published: September 2024

Background: Soil transmitted helminth(STH) infection is an infection caused by a group of nematode worms that is transmitted through parasite larvae or eggs that develop in the soil and then come into direct contact with humans. Helminth infections have a relationship with hygiene practices and environmental sanitation, in addition to adequate nutritional intake that makes the body's immune system strong so that it can prevent and fight infections, one of these important nutrients is protein.Objectives: To analyze the relationship between hygiene, environmental sanitation and protein intake on helminthiasis infection among toddlers aged 24-59 monthsMethods: Analytical research using a cross-sectional study in Atu Lintang District, Central Aceh Regency conducted in August-September 2023. The subjects used in this study were toddlers aged 24-59 months as many as 100 toddlers whose sampling used purposive sampling method. Data were collected by observation and interviewing the hygiene questionnaire, environmental sanitation and Semi quantitative Food Frequency Questionnaire (SQ-FFQ) to determine the adequacy of protein intake. Stool sample examination using the Kato Katz method. Data were analyzed using the Fisher Exact test with a significance level of 95%.Results: The results showed that 9% of toddlers were infected with STH and 91% were not infected with STH. Hand washing (p=0,002) and nail hygiene (p=0,000) were significantly associated with STH infection while footwear use (p=1,000), environmental sanitation (p=0,086) and protein intake (0.078) showed no significant association.Conclusion: In this study, the hygiene practices of hand washing and nail hygiene had a significant association with helminthiasis infection.
Pengaruh pemberian isocaloric fructose restriction dan kapsul daun kelor (Moringa oleifera) terhadap nilai antropometri pada kelompok obesitas usia 19-50 tahun
Authors: Mardhotillah Hanifah , Afifah Diana Nur , Pramono Adriyan
Published: September 2024

Background: The incidence of obesity has increased due to changes in lifestyle and eating patterns. Excessive consumption of fructose is a major contributor to obesity. Portion control can be used as a short-term strategy for weight loss. Limiting fructose consumption and using natural ingredients such as moringa leaves can be used in weight management in obese sufferes.Objectives: to determine the effect of administering isocaloric fructose restriction and moringa oleifera leaf capsules on antropometric values in the obese group.Methods: Single randomized controlled trial with pre-post test. The research conducted in Kuningan, West Java in August-October 2023 involving 24 subject with obesity. Subjects were divided into 2 groups, namely control (n=12) which was given isocaloric fructose restriction 1800 kcal and treatment group (n=12) given isocaloric fructose restriction 1800 kcal and Moringa oleifera capsule 2400mg/day for 9 days. Anthropometric measurements in the form of body weight, height and abdominal circumference were taken twice. Data analysis used Dependent t test at 95% CI.Results: There was a decrease in body weight and BMI before and after the intervention. The decrease in body weight and BMI was more significant in the treatment group (p<0,05).Conclusion: There is an effect of giving isocaloric fructose restriction and moringa leaf capsules on body weight and BMI.KeywordsObesity, Fructose, Moringa oleifera
Pengaruh kombinasi pijat oksitosin dan teknik marmet terhadap produksi ASI ibu postpartum primipara
Authors: Lubis Tika , Rofiasari Linda
Published: September 2024

Background: One of the causes of death is infection. Breast milk is the main food for newborn babies because it contains protective nutrients that can prevent infectious diseases in babies. One effort to improve the quality of breast milk is to stimulate the release of the hormone prolactin through oxytocin massage.Objective: To analyze the effect of a combination of oxytocin massage and the marmet technique on breast milk production in primiparous mothers.Method: This research design is quasi-experimental, using a one-group pre- and post-design research method and a sampling technique using consecutive sampling. The research was conducted in Bandung Regency on primiparous breastfeeding mothers. Univariate analysis to see the frequency and normality of the data (Shapiro Wilk test) and bivariate analysis for this study used a paired sample t test for 1 group with the Wilcoxon test to see the significance between before and after the intervention.Results: The results showed that the average breast milk production before the intervention was given was 24,22 ml, while after the intervention was given, it increased to 95,47 ml. So there was an increase in breast milk production of 71,25 ml after being given a combination of oxytocin massage and the marmet technique three times. The T-test results obtained a p-value of 0,000 < α (0,05), so it can be concluded that there is a significant difference in breast milk production before and after the intervention.Conclusion: There is a significant difference in breast milk production before and after the combination of oxytocin massage and the marmet technique. So, it is hoped that the smooth release of a mother's breast milk can increase the success of exclusive breastfeeding.
Efektifitas pemberian jahe, susu kedelai (soya) dan madu (jesoma) terhadap penurunan nyeri dismenore pada remaja putri
Authors: Rofiasari Linda , Lubis Tika , Sare Dewi Nurlaela , Gardenia Meli Sandra
Published: September 2024

Background: Dysmenorrhea is discomfort that can occur when experiencing menstruation which can have an impact on disrupting physical activities.Objectives: The focus of this research is to determine the effect of ginger, soy milk and honey (Jesoma) on reducing dysmenorrhea pain complaints.Methods: The research method used was Quasi-experimental with a one group pre-post test design approach. Sampling in this study used a purposive sampling technique with the sampling criteria being ready to be respondents, female students who were still active and experiencing dysmenorrhea and not experiencing secondary dysmenorrhea. The number of samples in this research was 32 female students at SMK 1 Ketapang. The pre-test in this research was carried out before the treatment and the post-test was carried out after the research by giving ginger, soy milk and honey (Jesoma).Result : Data analysis used the Wilcoxon test with an error rate of <0,05. The statistical results obtained a p-value of 0,000, so there was an effect of giving ginger, soy milk and honey (Jesoma) on reducing dysmenorrhea pain. Conclusion: In order to obtain the best intervention method in reducing dysmenorrhea, researchers can then carry out laboratory tests to determine the content of ginger, soy milk and honey (Jesoma) and combine it with several methods to obtain the best method in reducing dysmenorrhea.
Kawista fruit improves lung histopathology of rats exposed to cigarette smoke
Authors: Raharja Kristian Triatmaja , Susilawati Endang Fauziyah , Romadhoni Ita Fatkhur
Published: September 2024

Background: Free radicals in cigarettes can increase oxidative stress, exacerbating inflammation and activating alveolar macrophage cells. The phenolic components of kawista fruit act as antioxidants that reduce free radicals.Objective: To determine the effect of kawista fruit on alveolar macrophage activation in Wistar rats exposed to cigarette smoke (CS).Methods: The experimental research used a post-test only group design with a completely randomized design for 35 days. Wistar 25 male rats were divided into five groups: positive control (exposed to cigarette smoke), negative control (no treatment), O1, O2, and O3 (exposed to cigarette smoke and Kawista fruit sonde at doses O1=0,5, O2=0,6, and O3=0,7 g/kg BW). Data were analyzed using ANOVA one analysis of variance and LSD test.Results: Kawista fruit given preventively at doses of 0,6 and 0,7 g/kg BW could prevent the activation of lung alveolar macrophages in rats exposed to cigarette smoke (p=0,000). The lowest number of alveolar macrophages was seen at a dose of 0,7 g/kg BW.Conclusion:  Kawista fruit inhibited the increase in the number of alveolar macrophages and improved the lung histopathology of Wistar rats exposed to cigarette smoke.
Pengaruh lama fermentasi terhadap aktivitas antioksidan kombucha kunyit (Curcuma longa L.) sebagai minuman probiotik
Authors: Nafisah Eri , Ningrum Linda Wige , Budiandari Rahmah Utami , Hudi Lukman
Published: September 2024

AbstractBackground: Turmeric (Curcuma longa L.) is an herbal plant containing, the active compound curcumin, which has antioxidant and antibacterial properties, and its antioxidant content is equivalent to that of vitamin C and E. The active component of turmeric can be used as a probiotic drink, called kombucha. Kombucha is a tea fermented by bacteria and yeast which produce organic acids and antioxidants. Objectives: This study aims to formulate turmeric kombucha high antioxidant compound and analyze the antioxidant activity of tumeric kombucha.Methods: This research used one-factor RCBD, carried out starting in September 2023 four months in the Microbiology and Food Biotechnology Laboratory of the Bachelor of Food Technology Department, Universitas Muhammadiyah Sidoarjo. This research used fermentation time as factor (3,5,7,9,11,13,15) days and  analysis of antioxidant activity was analyzed by DPPH method. The resulting data will be processed using ANOVA followed by the BNJ test at the 5% level.Results: The study showed that the time fermentation showed  the antioxidant activity of turmeric kombucha was 57,58 ppm – 189,90 ppm. Increasing freephenol levels during fermentation will increases the antioxidant activity of kombucha.Conclusion: The fermentation time of turmeric kombucha affected its antioxidant activity.
Hipoalbuminemia sebagai prediktor mortalitas pada pasien Covid-19: Studi kasus di RSUD Prof. Dr Margono Soekarjo Purwokerto
Authors: Chasanah Imah Nur , Sri Rejeki Dwi Sarwani , Mars Wijayanti Siwi Pramatama
Published: September 2024

Background: Mortality in COVID-19 patients is related to several factors, including comorbidities, age, gender, increased D-dimer levels, elevated CRP levels, lymphopenia, leukocytosis, and hypoalbuminemia. Nevertheless, there is a scarcity of research on the impact of hypoalbuminemia on COVID-19 mortality, particularly in Indonesia.Objective: This study aimed to determine the effect of hypoalbuminemia, age, gender, and comorbid status on the mortality rate of COVID-19 patients at Prof. Dr. Margono Soekarjo Hospital.Methods: This research employed an observational analytic method, utilising a cohort design and survival analysis approach. The study was conducted at Prof. Dr. Margono Soekarjo Purwokerto Hospital in December 2023. The study utilised September 2021 medical records as secondary data. The data was analysed using Cox regression to determine the hazard ratio value. The results were deemed statistically significant if the p-value was < 0,05.Results: In September 2021, 217 individuals were hospitalised due to COVID-19. Out of all the patients, only 119 satisfied the specific criteria to be considered as study samples. A total of 49,6% of patients suffered from hypoalbuminemia. The mortality rate was 27,73%, with a higher incidence observed in patients with hypoalbuminemia (25 cases) compared to those without hypoalbuminemia (8 cases). The multivariate analysis revealed that patients with hypoalbuminemia had a 1,8-fold increased risk of mortality compared to patients without hypoalbuminemia (p = 0,007). The age (p = 0,08), gender (p = 0,38), and comorbid status (p = 0,22) of COVID-19 patients did not significantly affect mortality.Conclusion: COVID-19 patients with hypoalbuminemia had a 1,8-fold increased risk of mortality compared to those without hypoalbuminemia. Therefore, it can be inferred that hypoalbuminemia may serve as a prognostic indicator of mortality in COVID-19 patients. KeywordsAlbumin, COVID-19, death, hypoalbuminemia
TOURISM DEVELOPMENT STRATEGY BASED ON ENVIRONMENTAL SERVICES WITH INTEGRATED COASTAL ZONE MANAGEMENT (ICZM) TO ACHIEVE SUSTAINABLE DEVELOPMENT GOALS (SDGS) IN JOLOSUTRO BEACH OF BLITAR REGENCY INDONESIA
Authors:   Universitas Negeri Malang, Faculty of Social Science, Geography Department, Malang, Indonesia, SUMARMI Sumarmi , IRAWAN Listyo Yudha ,   Universitas Negeri Malang, Faculty of Social Science, Geography Department, Malang, Indonesia, ARINTA Dicky ,   Universitas Negeri Malang, Faculty of Social Science, Tourism Department, Malang, Indonesia, SUPRIANTO Agung ,   Universitas Negeri Malang, Faculty of Social Science, Social Science Department, Malang, Indonesia, KURNIAWATI Elya ,   Universitas Negeri Malang, Faculty of Social Science, Tourism Department, Malang, Indonesia, GRACIANI Helga ,   Universitas Negeri Malang, Faculty of Social Science, Tourism Department, Malang, Indonesia, MARLINA MARLINA ,   Universitas Negeri Makassar, Faculty of Social Science, Geography Department, Makassar, Indonesia, FAHIM Noraindah Binti Abdullah ,   Universiti Teknologi Malaysia, Faculty of Management, Johor Bahru, Malaysia, ARIF Mohamad ,   Universitas Negeri Malang, Faculty of Social Science, Geography Department, Malang, Indonesia, SHOLEHA dellia Wardatus ,   Universitas Negeri Malang, Faculty of Social Science, Geography Department, Malang, Malang, Indonesia, SHAHERANI Natasya ,   Universitas Negeri Malang, Faculty of Social Science, Geography Department, Malang, Malang, Indonesia
Published: September 2024

Tourism has become an alternative to improve community welfare. One activity trend is tourism based on an environmental services economy. The aim of this research is to design a tourism development strategy for Jolosutro Beach based on Environmental Services Economics with Integrated Coastal Zone Management (ICZM). This research uses a mixed design (mixed-method design) with a quantitative and qualitative approach. Data were analyzed using suitability analysis for tourism, SWOT, and Huberman interaction analysis. The research results show that the condition of Jolosutro Beach can be developed as environmental services-based tourism with Integrated Coastal Zone Management (ICZM).
Jayanta Mahapatra : A Silent Valediction
Authors: Pande Suresh Chandra
Published: September 2024

Sahitya Akademy award winner Jayanta Mahapatra - a luminary of Indian English Poetry was born on October 22,1928 in cuttack-Odisha and died there on August 28,2023.He belonged to a lower-middle class Indian family and after having gained Master's degree in Physics began teaching in different Government colleges in and across Odisha from 1949 to 1986.Meanwhile he authored 27 books of poems of which 7are in Odia and the rest in English. Best known for “Hunger” and “Indian Summer” Jayanta Mahapatra's “A Rain Of Rites” has an unusual enthrall and merit that I have attempted to interpret/ unravel briefly in my own culturally creative mode in this article besides commemorating his death. It is a book having imagery and symbolism as it's main thematic concern. The image of rain is used by the poet to symbolize fertility, creative impulse and destuctive power-potency.The rain as metaphor at various places never falls rather gets converted into light and then fails to accomplish its objective. Moreover, the whole series of poems suggest a process of purification by means of –strange stillness, quietude, solitude, mud-built houses, rivers, hills, trees and a vast stretch of landscapes. The soul of the poet is torn between good and evil, fair and foul and the ultimate judgement for deliverance, Mukti or salvation. As a whole-Rain Of Rites is an exacting lyric of troubled soul and undefined unhappiness that has left a good legacy :a gift.
Casting Shadows and Sunlight: Deconstructing the Cultural Kaleidoscope in the Select Poems of Jayanata Mahapatra
Authors: Zahra Andleeb
Published: September 2024

Jayanta Mahapatra, an eminent Indian poet writer in English language, captures the essence of cultural variations through the prism of his verse. His poetry transcends geographical boundaries, delving into the intricacies of identity, landscape and human experience. This paper explores the thematic motifs of cultural diversities and the interplay of regional identities in Mahapatra's poetry, revealing how he intricately weaves the complexities of cultural landscapes into his verses. In essence, Mahapatra's poetry serves as a reflection of the rich and varied cultural variations prevalent in India. His ability to encapsulate the essence of diverse cultural landscapes through his verse contributes significantly to the discourse on identity and regionalism making his works a significance contribution to the world of literature
Ethos of Orissa Landscape and Indian Sensibility In The Poems of Jayanta Mahapatra
Authors: Joshi Manu , Saxena Sharmila
Published: September 2024

Jayanta Mahapatra is one of the rising stars on the firmament of this species of Indian poetry. His works is of such intrinsic worth that he has already come to be regarded as the forth great poet in India. His ironic treatment with superb use of imagery with reference- to some of the more representative poems in the perspective of his commitment to a personal vision embedded in the materials practices, values of Oriya culture and rituals to some people of India. This paper attempts at the study of socio-political ethos of his native place, the images of locale, the social injustices that embodies the Oriya consciousness. The enormous contribution to the growth of Indian poetry in English , although he sporadically ventured in the realms of fiction; he will be remembered primarily for his poetry, his use of irony, imagery on love, sex, rituals and human misery voicing democratic mode.
DEVELOPMENT AND CHARACTERIZATION OF FAST DISSOLVING TABLETS FOR EMPAGLIFLOZIN
Authors: Gunda Dr. Raghavendra Kumar , Kumar JNS , Prasad ABS , Sandhya Bodepudi , Bhargavi Gajja , Padmaja KNVL , Praveen Sriram
Published: September 2024

Objective: The current study's objective is to develop and evaluate Fast dissolving tablets (FDT) for Empagliflozin. Empagliflozin, new class of oral hypoglycemic agent. It is indicated as an adjunct to exercise and diet to improve glycemic control in adult patients of type-2 diabetes.  It acts by inhibiting sodium glucose co-transporter (SGLT-2). Since oral absorption of empagliflozin from tablet is comparatively poor, hence an effort was made to enhance its absorption by formulating it as the fast dissolving tablet.  
Methods: Using various quantities of Kollidon-CL & Ac-Di-Sol as Superdisintegrants, FDT formulations of Empagliflozin were preared utilising the Direct Compression technique. Nine trials were developed and assessed for Pharmaceutical Product Performance.
Results: Findings indicate that all formulations meet the acceptance criteria, and kinetic modelling was applied to the in-vitro dissolution profiles.
Conclusion: The best formulation (F1) contained 6 mg of Kollidon-CL and 6 mg of Ac-Di-Sol showed promising results for obtain quicker disintegration and may produce patient compliance by means of rapid onset of action and preventing first pas effect too. Formulation (F1) follow first order, whereas release mechanism found to be non-fickian type (n= 0.762).
Vaginal Progesterone Gel versus Intramuscular Progesterone for Luteal Phase Support in Suboptimal Responders Undergoing Assisted Reproductive Cycles
Authors: Ulubaşoğlu Hasan , Bakay Kadir , Yavuzcan Ali , Güven Davut , Hatırnaz Şafak , Dahan Michael H.
Published: September 2024

Background: The purpose of this research is to compare the efficacy of 8% micronized vaginal progesterone gel (VAG) at 180 mg daily versus intramuscular progesterone (IMP) in oil at 100 mg daily for luteal phase support (LPS) after fresh single embryo transfer (SET) in Patient-Oriented Strategies Encompassing Individualized Oocyte Number (POSEIDON) Group 1b patients, and to ascertain whether the type of LPS predicts live birth in these patients. Methods: A total of 2420 assisted reproductive technology (ART) cycles performed in Ondokuz Mayıs University IVF Unit were analysed retrospectively. The data of POSEIDON Group 1b (unexpected suboptimal responders) who underwent antagonist in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles were included. All patients were categorized into groups according to the form of LPS, specifically VAG and IMP. Pregnancy, clinical pregnancy rate (CPR), live birth rate (LBR), spontaneous abortion rate and predictors of live birth (age, infertility duration, body mass index (BMI), type of progesterone used for luteal support, serum day 3 estradiol, serum progesterone on the day of human coryonic gonadotrophin (HCG), total duration of induction, serum estradiol on the day of HCG, the total number of oocytes retrieved, number of mature oocytes obtained, average gonadotropin dose per day, total gonadotropin dose administered and total number of embryos retrieved) were analyzed. Results: There was no statistically significant difference between the micronized VAG and IMP groups in terms of age (31 (23–35) vs. 27 (23–35), p = 0.319). There were no statistically significant differences in pregnancy outcomes between the two groups concerning CPR per transfer (70.6% vs. 75.6%; p = 0.364), ongoing pregnancy per cycle (36.2% vs. 39.5%; p = 0.577), and LBR per cycle (34.4% vs. 36.1%; p = 0.785). The spontaneous abortion rates (36.2% vs. 36.8%; p = 0.921) were similar between VAG and IMP groups. The type of LPS did not emerge as a parameter predicting pregnancy (odds ratio (OR): 0.718, 95% confidence interval (95% CI): 0.652–1.313, p = 0.451), clinical pregnancy (OR: 0.598, 95% CI: 0.592–1.289, p = 0.562) and live birth (OR: 0.802, 95% CI: 0.661–1.202, p = 0.580). The logistic regression analysis aimed at assessing the influence of confounding factors, namely age, BMI, and duration of infertility on pregnancy rate, CPR and LBR, did not reveal statistically significant results (p > 0.05). Conclusions: VAG 180 mg daily provide similar pregnancy outcomes compared to 100 mg daily IMP in POSEIDON Group 1b patients undergoing antagonist fresh IVF/ICSI cycles.
Application of Shear Wave Elastography in the Diagnosis and Evaluation of Cervical Cancer
Authors: Cao Lina , Shen Peipu , Wei Min , Du Wensheng , Lu Xiaoyuan
Published: September 2024

Background: Our goal was to add an auxiliary examination method for the detection of cervical cancer, and to further explore its application value in clinical staging and treatment. Methods: Shear wave elastography (SWE) technique was used to examine the cervical hardness of patients, and the maximum, mean and minimum values of elastic modulus were recorded, with differences being compared. The area under the receiver operating characreristic (ROC) curve and diagnostic efficacy of elastic modulus were compared with Medcalc software. The differences of elastic modulus values under different parameters were compared in the cervical cancer group. Results: The mean, maximum and minimum values of the cervical cancer group were all the highest, and the differences were statistically significant. The area under the ROC curves were 0.925, 0.909 and 0.873, respectively. For the mean and maximum values, the Youden indexes were 0.79 and 0.72, the positive likelihood ratios were 21.74 and 19.97, and the negative likelihood ratios were 0.18 and 0.25. The optimal cut-off point was 82.2 kilpoascal (kPa) for maximum value and was 66.5 kPa for mean value. Elastic modulus were all significantly different according to International Federation of Gynecology and Obstetrics (FIGO) stages and tumor lesion size. Also, elastic modulus of cervical cancer patients before and after radiotherapy were statistically significant. Conclusions: SWE technology has an application value in the detection of cervical cancer. The mean and maximum values have higher diagnostic accuracy. SWE technology also has potential clinical application value in the clinical staging and treatment of cervical cancer, but further studies with larger sample sizes are needed.
Vitamin D May not be Crucial: Association between Serum Vitamin D Level and Pregnancy-Induced Hypertension-A Meta-Analysis
Authors: Cui Changxing , Huang Xiaoyan , Cui Yuanqing , Jiang Shaochang , Yao Xiaoyan , Li Xuelong
Published: September 2024

Background: The association between vitamin D and pregnancy-induced hypertension (PIH) remains contentious. The aim of our study was to evaluate the existence of an association between serum vitamin D levels and the incidence of PIH. Methods: We conducted a literature search in PubMed, the Cochrane Library, and Embase databases in June 2024 using the following search terms: 25-hydroxyvitamin D (25(OH)D), Vitamin D, 1,25(OH)2D, VD, 1,25-dihydroxyvitamin D or 25(OH)D, combined with PIH. Two reviewers independently screened the literature based on inclusion and exclusion criteria. Data were then extracted and assessed for quality. Comparisons were made between the highest and lowest categories of serum vitamin D levels. Relative risks (RRs) and 95% confidence intervals (CIs), adjusted for multivariable effects, were pooled using a random-effects model. A two-stage dose-response meta-analysis was used to evaluate the trends. Results: 17 studies met the inclusion criteria. Of these, 11 prospective studies investigated the relationship between vitamin D levels and gestational hypertension, involving 8834 events and 17,104 participants. The results showed that vitamin D was only marginally associated with hypertensive disorders in pregnancy (summary RR = 0.99; 95% CI: 0.97–1.02; I2 = 67.5%; p = 0.001). However, 6 case-control studies investigated the relationship between vitamin D levels and gestational hypertension, involving 80,814 events and 330,254 participants. The results showed that vitamin D is not associated with pregnancy hypertensive disorders (summary RR = 1.09; 95% CI: 0.84–1.41; I2 = 75.4%; p = 0.001). In the subgroup analysis, the pooled effect of 25-hydroxyvitamin D (25(OH)D) showed a slight association with gestational hypertension (pooled RR = 0.99; 95% CI: 0.96–1.02; I2 = 72.6%; p = 0.000). The dose-response analysis showed that increasing vitamin D doses are marginally associated with a decrease in the incidence rate. Conclusions: Our research suggests that the risk of PIH may not be related to the vitamin D levels. Our research supports the hypothesis that gestational hypertension may not be associated with low levels of vitamin D, indicating that the role of vitamin D may not be significant.
Forceful Milk Ejection Reflex and Tongue-Tied Neonates’ Breastfeeding Behaviour
Authors: Muresan Marta , Muresan Sorana
Published: September 2024

Background: Forceful milk ejection reflex in mothers and tongue-tie in infants often cause breastfeeding difficulties. Our purpose was to observe the breastfeeding behavior of tongue-tied neonates, in the presence of forceful milk ejection of their mother versus tongue-tied infants without forceful milk ejection or in the presence of forceful milk ejection in non-tongue-tied infants. Methods: This is an observational, prospective, cohort study. The study was undertaken at the Lactation Institute, in Cluj-Napoca, Romania, between 2012 and 2018. We studied the breastfeeding behavior of neonates attending the Institute. The study includes 80 tongue-tied new-borns of mothers with forceful milk ejection (Group I), compared with the breastfeeding behavior of 40 tongue-tied neonates of mothers with normal let-down (Group II) and 29 neonates without tongue-tie in the presence of forceful let-down (Group III). Descriptive statistics, including numbers and percentages, and SAS software were utilized. Categorical data are presented as counts and percentages and analyzed with chi-square tests, utilizing R and Python software. Statistical significance was set at p-value ≤ 0.001, in some cases at p-value ≤ 0.05 using a chi-square test, sometimes validating the results with Fisher’s Exact Test. Proportional Analysis and Post-Hoc Analysis (pairwise Z-tests for proportions) were also carried out to demonstrate the frequency of cases. Results: From Group I, the majority (92.50%) were breastfed effectively in the first week, significantly different from infants in Group II (20%), where frenotomy was more frequent (92.50%) and performed mainly for suckling difficulties (86.48%). The difficult latch had a higher percentage in infants in Group II (92.50%). “Reflux-like syndrome” (93.10%), breast refusal (24.13%), and, choking (93.10%) were more frequent in infants in Group III. Conclusions: The majority of tongue-tied infants, whose mothers had early forceful milk ejection, were able to effectively breastfeed after birth. In this cohort, frenotomy might not be necessary or could be postponed. The presence of tongue-tie only, without fast milk flow, led to suckling difficulties and the need for frenotomy. Reflux-like syndrome, choking, and breast refusal were more present in infants without tongue-tie but with fast milk flow.
Evaluation Effectiveness of Pre-Treatment Combined with Ultrasound-Guided Suction Curettage in Cesarean Scar Pregnancy: A Retrospective Cohort Study
Authors: Gan Yanqiong , Jiang Hao , Zhou Yuqin , Chen Zhaoxia
Published: September 2024

Background: The objective of this study is to assess the effectiveness of ultrasound-guided suction curettage (UGSC) in conjunction with or without high-intensity focused ultrasound (HIFU) or uterine artery embolization (UAE) for the treatment of three distinct types of cesarean scar pregnancy (CSP). Methods: This was a retrospective study that included subjects diagnosed with CSP in a single tertiary referral centre from January 2015 to December 2020. UGSC associated with/without pretreatment was offered to CSP patients according to the location of pregnancy sac, the depth of implantation and the surrounding blood flowing. The data about baseline characteristics and outcome parameters were analyzed across the three groups. Results: The present study included 434 patients diagnosed with CSP. The majority were cured without severe complication except one case accepted blood transfusion. The baseline data from the three groups did not differ statistically. The UGSC group had the same successful rate of treating type I CSP with a shorter hospital stay (χ2 = 51.771, p = 0.000) compared to the HIFU or UAE groups. Type II CSP presented marked less blood loss (Z = –2.464, p = 0.014) and higher decline ration of beta-human chorionic gonadotropin (β-HCG) (Z = –4.728, p = 0.000) in HIFU group. Treatment success was high in patients with type III CSP in both the HIFU and UAE groups, but the rate of β-HCG decline was higher in the HIFU group (Z = –2.255, p = 0.024). Conclusions: This study suggests that UGSC is an effective and safe option for patients with low-risk CSP. HIFU or UAE combined with UGSC has higher efficacy in high-risk CSP. And HIFU may be superior in reducing bleeding and increasing the rate of β-HCG decline.
Aurora A Kinase Begins to Localize to the Centrosome in the S-phase of the Cell Cycle in the XL2 Cell Line
Authors: Uzbekov Rustem , Uzbekova Svetlana , Severin Fedor , Prigent Claude , Arlot-Bonnemains Yannick
Published: September 2024

Background: The centrosome is one of the principal cell hubs, where numerous proteins important for intracellular regulatory processes are concentrated. One of them, serine-threonine kinase 6, alias Aurora A, is involved in centrosome duplication and mitotic spindle formation and maintenance. Methods: Long-term vital observations of cells, immunofluorescence analysis of protein localization, synchronization of cells at different phases of the cell cycle, Western blot analysis of protein content were used in the work. Results: In this study, we investigated the dynamics of Aurora A protein accumulation and degradation in the XL2 Xenopus cell line during its 28-hour cell cycle. Using Western blot and immunofluorescence analyses, we demonstrated that Aurora A disappeared from the centrosome within one hour following mitosis and was not redistributed to other cell compartments. Using double Aurora A/Bromodeoxyuridine immunofluorescence labeling of the cells with precisely determined cell cycle stages, we observed that Aurora A reappeared in the centrosome during the S-phase, which was earlier than reported for all other known proteins with mitosis-specific centrosomal localization. Moreover, Aurora A accumulation in the centrosomal region and centrosome separation were asynchronous in the sister cells. Conclusions: The reported data allowed us to hypothesize that Aurora A is one of the primary links in coordinating centrosome separation and constructing the mitotic spindle.
Developing and Verifying an Effective Diagnostic Model Linked to Immune Infiltration in Stanford Type A Aortic Dissection
Authors: Huang Xiaoyan , Zhang Guoan , Feng Yangmeng , Zhao Xiangrong , Li Yaping , Liu Fuqiang , Dong Yihan , Sun Jingying , Xu Cuixiang
Published: September 2024

Background: The deadly cardiovascular condition known as Stanford type A aortic dissection (TAAD) carries a high risk of morbidity and mortality. One important step in the pathophysiology of the condition is the influx of immune cells into the aorta media, which causes medial degeneration. The purpose of this work was to investigate the potential pathogenic significance of immune cell infiltration in TAAD and to test for associated biomarkers. Methods: The National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database provided the RNA sequencing microarray data (GSE153434, GPL20795, GSE52093). Immune cell infiltration abundance was predicted using ImmuCellAI. GEO2R was used to select differentially expressed genes (DEGs), which were then processed for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Additionally, hub genes linked to immune infiltration were found using functional and pathway enrichment, least absolute shrinkage and selection operator (LASSO), weighted gene co-expression network analysis (WGCNA), and differential expression analysis. Lastly, hub genes were validated and assessed using receiver operating characteristic (ROC) curves in the microarray dataset GSE52093. The hub gene expression and its connection to immune infiltration in TAAD were confirmed using both animal models and clinic data. Results: We identified the most important connections between macrophages, T helper cell 17 (Th17), iTreg cells, B cells, natural killer cells and TAAD. And screened seven hub genes associated with immune cell infiltration: ABCG2, FAM20C, ELL2, MTHFD2, ANKRD6, GLRX, and CDCP1. The diagnostic model in TAAD diagnosis with the area under ROC (AUC) was 0.996, and the sensitivity was 99.21%, the specificity was 98.67%, which demonstrated a surprisingly strong diagnostic power of TAAD in the validation datasets. The expression pattern of four hub DEGs (ABCG2, FAM20C, MTHFD2, CDCP1) in clinic samples and animal models matched bioinformatics analysis, and ABCG2, FAM20C, MTHFD2 up-regulation, and the of CDCP1 down-regulation were also linked to poor cardiovascular function. Conclusions: This study developed and verified an effective diagnostic model linked to immune infiltration in TAAD, providing new approaches to studying the potential pathogenesis of TAAD and discovering new medication intervention targets.
A New Target for Hepatic Fibrosis Prevention and Treatment: The Warburg Effect
Authors: Pan Meng , Li Huanyu , Shi Xiaoyan
Published: September 2024

Hepatic fibrosis is a major public health problem that endangers human wellbeing. In recent years, a number of studies have revealed the important impact of metabolic reprogramming on the occurrence and development of hepatic fibrosis. Among them, the Warburg effect, as an intracellular glucose metabolism reprogramming, can promote the occurrence and development of hepatic fibrosis by promoting the activation of hepatic stellate cells (HSCs) and inducing the polarization of liver macrophages (KC). Understanding the Warburg effect and its important role in the progression of hepatic fibrosis will assist in developing new strategies for the prevention and treatment of hepatic fibrosis. This review focuses on the Warburg effect and the specific mechanism by which it affects the progression of hepatic fibrosis by regulating HSCs activation and KC polarization. In addition, we also summarize and discuss the related experimental drugs and their mechanisms that inhibit the Warburg effect by targeting key proteins of glycolysis in order to improve hepatic fibrosis in the hope of providing more effective strategies for the clinical treatment of hepatic fibrosis.
High Mobility Group Box-1 (HMGB1), a Key Mediator of Cognitive Decline in Neurotrauma with a Potential for Targeted Therapy: A Comprehensive Review
Authors: Navaseelan Locshiny , Retinasamy Thaarvena , Shaikh Mohd. Farooq , Arulsamy Alina
Published: September 2024

Neurotrauma plays a significant role in secondary injuries by intensifying the neuroinflammatory response in the brain. High Mobility Group Box-1 (HMGB1) protein is a crucial neuroinflammatory mediator involved in this process. Numerous studies have hypothesized about the underlying pathophysiology of HMGB1 and its role in cognition, but a definitive link has yet to be established. Elevated levels of HMGB1 in the hippocampus and serum have been associated with declines in cognitive performance, particularly in spatial memory and learning. This review also found that inhibiting HMGB1 can improve cognitive deficits following neurotrauma. Interestingly, HMGB1 levels are linked to the modulation of neuroplasticity and may offer neuroprotective effects in the later stages of neurotraumatic events. Consequently, administering HMGB1 during the acute phase may help reduce neuroinflammatory effects that lead to cognitive deficits in the later stages of neurotrauma. However, further research is needed to understand the time-dependent regulation of HMGB1 and the clinical implications of treatments targeting HMGB1 after neurotrauma.
Knockdown of IGF2BP3 Down-Regulates PDCD4 Levels to Attenuate Hypoxic-Ischemic Brain Damage
Authors: Chen Yuxia , Fang Xiaoyi , Liu Huayan , Fan Qianqian
Published: September 2024

Background: Hypoxic-ischemic brain damage (HIBD) is a prevalent brain injury with high mortality and morbidity. It results from hypoxia and ischemia of the brain due to various perinatal factors. A previous study showed that knockdown of programmed cell death factor 4 (PDCD4) could reduce infarction injury resulting from ischemia/reperfusion injury. However, exact mechanism by which PDCD4 acts in HIBD is not yet understood. Our aim in present investigation was to investigate the function and mechanism of PDCD4 in alleviating HIBD. Methods: An HIBD model was developed using neonatal rats. After 48 h of modeling, short-term neurological function was evaluated and the brain tissue removed for assessment of cerebral infarct volume and brain water content (BWC). A cell model of oxygen glucose deprivation/reoxygenation (OGD/R) was also constructed. Overexpression or knockdown of insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) or PDCD4 was performed in pretreated cells. Results: The geotaxis reflex time, cerebral infarct volume, and BWC all increased after HIBD in this neonatal rat model. Additionally, the levels of PDCD4 and of the N6-Methyladenosine (m6A) reader protein IGF2BP3 were increased in HIBD rats and OGD/R-stimulated pheochromocytoma (PC12) cells relative to controls. Moreover, OGD/R-stimulated pheochromocytoma PC12 cells showed decreased cell viability, increased apoptosis, and elevated Interleukin 6 (IL-6), Interleukin 1 β (IL-1β), and tumor necrosis factor-α (TNF-α) contents. These features were reversed after knocking down IGF2BP3. The interaction between IGF2BP3 protein and PDCD4 mRNA was confirmed by RNA immunoprecipitation and RNA pull-down assays. Furthermore, knockdown of IGF2BP3 in OGD/R-stimulated PC12 cells reduced cell damage via down-regulation of PDCD4. Finally, the IGF2BP3/PDCD4 axis alleviated OGD/R-induced cell injury in primary cortical neurons (PCNs). Conclusions: PDCD4 and m6A reader protein IGF2BP3 were up-regulated in an HIBD neonatal rat model. Knockdown of IGF2BP3 in OGD/R-stimulated PC12 cells or PCNs alleviated cell damage through reducing PDCD4.
ZNF429 Participates in the Progression of Coronary Heart Disease through Regulating Inflammatory and Adhesive Factors
Authors: Wang Hao , Wu Bo , He Xueqin , Li Wei , Guan Wenqi
Published: September 2024

Background: Coronary heart disease (CHD) is an intricate and multifaceted cardiovascular disorder that contributes significantly to global morbidity and mortality. Early and accurate identification and diagnosis of CHD are paramount to ensuring patients receive optimal therapeutic interventions and satisfactory outcomes. Methods: Data on CHD gene expression were obtained from the Gene Expression Omnibus (GEO) repository and potential hub genes were screened through gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), weighted gene co-expression network analysis (WGCNA), and least absolute shrinkage and selection operator (LASSO) analyses. Functional validation of these hub genes was conducted by interfering with them in human umbilical vein endothelial cells (HUVECs). Cell proliferation and apoptosis were assessed through cell counting kit-8 (CCK-8) and flow cytometry assays, respectively, while enzyme-linked immunosorbent assay (ELISA), quantitative polymerase chain reaction (qPCR), Western blot, and immunofluorescence were used to measure the expression of key indicators. Results: We identified 700 upregulated differentially expressed genes (DEGs) and 638 downregulated DEGs in CHD, and utilized LASSO analyses to screen disease potential biomarkers, such as zinc finger protein 429 (ZNF429). Interference with ZNF429 in HUVECs mitigated the CHD-induced decrease in cell proliferation and increase in apoptosis. Moreover, the expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), cluster of differentiation 62E (CD62E), and cluster of differentiation 62P (CD62P) was reduced, leading to decreased cellular inflammation and adhesion. Conclusions: CHD-associated biomarker ZNF429 was identified through bioinformatics analysis to potentially regulate the expression of inflammatory factors IL-6, IL-1β, and TNF-α, along with adhesion molecules ICAM-1, VCAM-1, CD62E, and CD62P. This modulation influence was subsequently found to impact the progression of CHD. These findings offered valuable insights into potential targets for further investigation and therapeutic interventions for CHD management.