bluuebunny/crossref_metadata_2025 huggingface dataset:
XIX. YÜZYIL SONLARI VE XX. YÜZYIL BAŞLARINDA AYDIN SANCAĞI’NDA EĞİTİM
Authors: GÜNEŞ Müslime
Published: May 2023

Çok köklü bir tarihe ve kültürel mirasa sahip olan Aydın’ın, sosyal, ekonomik, siyasal alanlarda geçirmiş olduğu aşamalar kadar, eğitim alanında geçirmiş olduğu değişim ve dönüşüm de oldukça önemlidir. Kentlerin kendi etki alanlarında yaşayanlara sunduğu sosyal, ekonomik ve kültürel olanaklar yalnızca orada yaşayanları değil çevre yerleşmelerden de ilgi ve nüfus çeker. Aydın'ın sahip olduğu ekonomik zenginlik, ticaret yolları üzerinde bulunuşu, büyük bir ihraç limanı olan İzmir'in ard bölgesinde önemli bir tarımsal hinterlanda sahip oluşu onu bir çekim merkezi haline getirmiştir. Aydın'ın sözü edilen alanlarına ilişkin oldukça önemli araştırmalar yer almakla birlikte; eğitim kurumları ve faaliyetleri hakkında yapılan akademik çalışmaların sınırlı kaldığını görmekteyiz. Eğitim sisteminin tam olarak anlaşılabilmesi için vilayet ve sancak ölçeğinde mikro düzeyde araştırmalara ihtiyaç duyulduğu bir gerçektir .Bu araştırma Cumhuriyet öncesi dönemde Aydın Sancağındaki maarif komisyonlarını, okullar, öğrenciler, öğretmen ve görevli kadroları, medreseler ve gayrimüslimlere ait okulların durumunu, Osmanlı arşiv belgeleri, Osmanlı maarif salnameleri, Aydın Vilayet salnameleri ve basılı eserlere dayanarak değerlendirmeyi amaçlamaktadır.. Araştırmanın verileri, tarihsel araştırmanın sıklıkla kullandığı yöntemlerden doküman incelemesi yoluyla elde edilmiştir.
Factores asociados a la reacción de estrés agudo en gestantes con hiperémesis gravídica hospitalizadas en el Instituto Nacional Materno Perinatal, Lima, Perú (2021)
Authors: Silva-Dominguez Daniel , Moreno-Valles Luisa , Huarancca-Berrocal Isabel , Manco-Avila Ermes , Torrejon-Fernandez Cynthia , Barba-Aymar Gloria , Orihuela-Salazar Jimmy , Prado-Duran Hanna
Published: May 2023

Objetivo. Determinar los factores que se encuentran asociados a la hiperémesis y reacción de estrés agudo en gestantes hospitalizadas con hiperémesis gravídica en el Instituto Nacional Materno Perinatal, durante el 2021. Materiales y Métodos. Esta investigación corresponde a un estudio analítico, observacional, de tipo casos y controles, donde se recopilaron las historias clínicas de pacientes gestante hospitalizadas por hiperémesis gravídica atendidas por el servicio de psicología las cuales presentaron reacción de estrés agudo cumpliendo los criterios de inclusión y exclusión. Se realizó un análisis de frecuencias y porcentajes de las diferentes variables y un análisis multivariado para encontrar los factores significativos (p<0.05) y el cálculo de los Odds Ratio (OR) con un nivel de confianza del 95%; este modelo fue evaluado mediante una regresión logística múltiple. Resultados. Se encontró relación entre la planificación del embarazo (p<0.044, β=0.957 y OR=2.6047) y el rechazo a las náuseas (p<0.028, β=1.774 y OR=5.893) en gestantes con hiperémesis gravídica. Se halló relación directa entre la reacción de estrés agudo en gestantes con hiperémesis gravídica, respecto a la perdida gestacional (p<0.043, β=0.933 y OR=2.542) y hospitalización en zona COVID-19 (p<0.010, β=1.467 y OR=4.335); e inversa, respecto a la aceptación del embarazo (p<0.014, β=-1.515 y OR=0.220). Conclusión. Se encontraron dos variables asociadas a la presencia de hiperémesis gravídica (planificación del embarazo y el rechazo a las náuseas). La reacción de estrés agudo se ha relacionado con dos factores asociados a las pacientes (perdida gestacional y aceptación del embarazo) y una asociada a la pandemia (hospitalización en zona COVID-19) las cuales explican el 32% de la varianza.
Rotura hepática espontánea en puérpera sin enfermedad hipertensiva del embarazo ni enfermedad hepática: Reporte de caso
Authors: Guevara-Ríos Enrique , Torres-Contreras Hayder , Novoa-Reyes Rommy , Diaz-Seminario Alberto , Llocclla-Kano Patricia , Puerta-Caldas Mercedes , Justo-Calle Rosa
Published: May 2023

La rotura hepática es una complicación grave de un embarazo con una alta morbilidad y mortalidad, está relacionada frecuentemente con la preeclampsia; excepcionalmente se puede presentar en una gestante sin preeclampsia o síndrome HELLP. Se presenta un caso de una gestante sin preeclampsia ni enfermedad hepática previa que desarrolla una rotura hepática en el puerperio, es diagnosticada oportunamente y manejada quirúrgicamente. La paciente evolucionó favorablemente.
Estudo comparativo da capacidade de corte e resistência de instrumentos rotatórios de Ni–Ti Waveone® Gold e Trunatomy® durante o preparo do canal radicular
Authors: Haddad Filho Miguel Simão , Tognetti Valdinéia Maria , Pedron Irineu Gregnanin , Godoi Beatriz Corina , Shitsuka Caleb , Medeiros João Marcelo Ferreira de
Published: May 2023

O presente estudo avaliou comparativamente a capacidade de corte e resistência de duas sequências de instrumentação considerando a diferença de peso após o preparo do canal radicular simulado em blocos de resina acrílica transparente com ângulo de curvatura padronizado em 45°. Os recursos foram submetidos ao máximo de dez ciclos de esterilização e uso. Os blocos foram pesados em balança digital de máxima precisão antes e após o preparo, seguido da verificação de perda de peso no tocante a capacidade de corte dos instrumentos mediante interferência de múltiplos usos e autoclavagem, já a análise da resistência pode ser constatada mediante quantidade de blocos utilizados para cada protocolo, até o limite máximo de dez blocos por grupo ressalvado ocorrência de fratura. Pode-se concluir que o sistema TruNatomy® foi mais efetivo no quesito resistência considerando sua atuação nos dez blocos pretendidos, diferente do protocolo Proglider® mais WaveOne Gold Primary® onde houve fratura no quinto uso. Quanto a capacidade de corte apresentou ligeira vantagem do instrumento WaveOne Gold Primary® (média de 4 blocos=3,4629g) em relação ao TruNatomy® (média de 4 blocos=3,4506g), porém sem significado estatístico.
Programa Saúde na Escola: ação em saúde mental com alunos do ensino médio - relato de experiência
Authors: Luna Nataly Maria Bezerra de , Abrantes Ester Emanuele , Silva Ana Karolina Alves da , Costa Maria Vitória Ferreira da , Maia Débora Monte Carlos Barbosa , Silva Maitê Targino da , Silva Luisiane de Avila
Published: May 2023

Objetivo: o presente estudo visa expor a experiência de acadêmicos de medicina com uma ação educativa abordando e discutindo sobre saúde mental e valorização da vida dos estudantes no período pré-vestibular a partir de uma dinâmica simbólica realizada com base no Programa Saúde na Escola (PSE). Este programa possui o intuito de realizar uma parceria entre a comunidade escolar e a atenção primária. Metodologia: Este estudo caracteriza-se como um relato de experiência da realização de uma dinâmica por um grupo de graduandos de medicina, abordando o tema da ansiedade que antecede o vestibular e a valorização da vida. Resultados: Notou-se grande prevalência de alunos com sintomas de ansiedade relacionada com o vestibular e desconhecimento das formas de buscar ajuda, como o Centro de Valorização da Vida (CVV) e os profissionais de saúde que compõem a rede de Saúde Mental. Também foi abordado as diferenças entre ansiedade normal e patológica. Conclusão: Conclui-se que, de fato há um índice de instabilidade emocional nos pré-vestibulandos, uma vez que a maioria se identificou com os sentimentos ruins durante a atividade. Além disso, notou-se a necessidade da ampliação dos PSE para a docência, já que a ansiedade também os acomete, principalmente pelo alto índice de estresse cotidiano.
Fisioterapia oncológica nos cuidados paliativos à atenção à mulher com câncer ginecológico: um estudo de revisão
Authors: Araujo Elis Raissa da Silva , Araújo Iriciane Itale Pontes , Ferro Thauan Narciso de Lima
Published: May 2023

Introdução: A fisioterapia tem muito a contribuir para o cuidado do paciente oncológico. Em se tratando dos tumores ginecológicos, que compreendem vulva, vagina, colo do útero, endométrio, tuba uterina e ovário, diante do diagnóstico de doença que ameace a vida, preconiza-se abordagem por meio dos cuidados paliativos, a fim de prevenir e aliviar sintomas. Objetivo: o presente trabalho possui por objetivo realizar uma revisão sistemática sobre a eficácia da fisioterapia como tratamento adjunto em pacientes com câncer ginecológico. Trata-se de uma Revisão Sistemática da literatura. Metodologia: A busca dos artigos foi realizada nas seguintes bases de dados on-line: Biblioteca Virtual em Saúde (BVS) que inclui as bases SciELO (Scientific Eletronic Library On Line), Lilacs (Literatura Latino-Americana e do Caribe em Ciências da Saúde) e Pubmed. Resultados: Os resultados demostraram que as intervenções fisioterapêuticas para pacientes submetidas a tratamento de câncer de mama e ginecológico têm níveis variáveis de evidência para contribuir para a prevenção ou atenuação do comprometimento físico-funcional, sintomas causados pelos tratamentos. Conclusão: Conclui-se que, com base na literatura de pesquisa existente sobre o impacto da fisioterapia na mulher portadora de câncer ginecológico, fica claro que as diferentes abordagens utilizadas são benéficas à melhoria da qualidade de vida do paciente.
Utilização oral do ácido tranexâmico aplicado à hiperpigmentação cutânea: uma revisão
Authors: Silva Cristiane Maria Barbosa da , Alves Natália Crisostimo , Vasconcelos Tibério Cesar Lima de
Published: May 2023

A hiperpigmentação cutânea, comumente chamada de melasma, uma condição sem causa clara localizada na face e caracterizada por manchas irregulares com elevado grau de vascularização, coloração marrom de alta intensidade, resultantes da deposição de melanina derivada da oxidação da tirosina nos melanócitos localizados na camada basal da epiderme. Mesmo afetando ambos os sexos, sua ocorrência maior é em mulheres. As análises realizadas apontam que o ácido tranexâmico apresenta-se como uma opção aos cuidados no melasma, mesmo os tratamentos disponíveis não tendo eficácia absoluta. O objetivo deste artigo é relatar a ação do ácido tranexâmico e suas vias de administração oral, com ou sem outros meios de tratamento. A metodologia utilizada para o estudo revisão integrativa de estudos duplo-cego. Foram selecionados 86 artigos entre os anos de 2012 e 2022, os quais foram selecionados após aplicados os critérios de exclusão e inclusão. Os resultados indicam que o ácido tranexâmico oral é eficaz e apresenta baixos efeitos colaterais, o que favorece a adesão dos pacientes ao tratamento. Concluindo que essa terapia pode ser uma opção promissora para o tratamento do melasma.
Worker adjustment in the digital age
Authors:   , Röttger Ronja Christine
Published: May 2023

Income inequality has been rising in many advanced economies over the past four decades. However, the underlying sources of rising income inequality are still not fully understood. For this reason, this dissertation investigates two drivers of rising income inequality: digital technologies and the role of firms. I conduct four empirical studies focusing on two drivers of rising earnings inequality. The first half of the dissertation contributes to the large strand of literature investigating the impact of digital technologies on the labor market. Previous research mainly focused on economy-wide outcomes of digital technologies, however less is known about the impact on individual workers. Who are the workers that win and who are the workers that lose from digital technologies? How do the wages of college-educated workers develop in technology-intensive careers? The second half of the dissertation contributes to the research on the role of firms in rising earnings inequality. Here, I examine the role of firm-specific technology in firm wage premiums and the rise in high-wage workers sorting to high-wage firms. In addition, I quantify the wage-setting power of firms in the Netherlands.
EMOTIONAL BONDS IN PERCEIVING EMOTIONAL CLOSENESS AND PERSONAL WELL-BEING IN PARTNER RELATIONSHIPS AND GENDER DIFFERENCES DURING ADOLESCENCE
Authors: MIČKOVÁ ZUZANA
Published: May 2023

Interactive relationships are necessary for personality formation in all stages of life, but adolescence is a period of finding a partner; and we are interested to find out how the emotional bond with a partner reflects emotional closeness in comparison with individuals without a current relationship. In this study, we will focus our attention on the relationships of adolescents in accordance with family and partners, as well as their personal well-being. We are interested to what extent there are differences in the subjective living and the need to start a relationship across both genders. The research sample consisted of university students of humanities and economic studies (N= 98, M=23, 97; 19- 24 years) in Trnava and in Galanta (UCM, BIVŠ, UJAK). A substantial proportion of the sample represents female adolescents who are in a romantic relationship for at least a year
THE RELATIONSHIP BETWEEN FAMILY FACTORS AND SOMATIC PROBLEMS IN ADOLESCENCE
Authors: MIČKOVÁ ZUZANA , BLATNÝ MAREK , HRDLIČKA MICHAL
Published: May 2023

The paper examined the presence of somatic symptomatology in relation to the perception of parental education, emotions and sense of security in the family environment. Those surveyed consisted of 4,847 adolescents The defined family factors consisted of inconsistency of education, parental interest, supervision, affection and a sense of security within the family environment and these were examined in relation to selected somatic issues through the SAHA methodology. The results of the correlation analysis indicated that the quality of these family factors is related to the symptoms of somatic problems of adolescents. A positive correlation between somatic symptoms and inconsistency of education and a negative correlation between somatic symptoms and a sense of security at home were observed.
MOTIVATION OF CZECH STUDENTS OF SECONDARY TECHNICAL SCHOOLS TO STUDY ENGINEERING
Authors: VICHERKOVÁ DANA , MALACH JOSEF
Published: May 2023

The main aim of the paper is to introduce the recent findings of the motivation of secondary technical schools’ students in the field of engineering. Technological progress requires higher quality and quantity of human resources working in engineering field, however, the interest of youth in studying the field is falling steadily. A project “Education in engineering and its optimisation for the needs of the labour market” led by the Technical Agency of the Czech Republic (TAČR), which is implemented by the Faculty of Education at the University of Ostrava together with the National Engineering Cluster is focused on discovering the factors influencing the choice of these fields, students´ satisfaction with their studies, study aspiration and manifestation of an in-depth interest in technical fields. The results of a pilot research confirm that almost one half of the students believe they possess aptitudes for engineering professions.
GENDER-INCLUSIVE LANGUAGE IN THE LINGUISTIC LANDSCAPE OF BANSKÁ BYSTRICA DURING THE CORONAVIRUS PANDEMIC
Authors: ŠTEFAŇÁKOVÁ JANA
Published: May 2023

The present study deals with the use of gender-inclusive language on hygiene notices that were placed on public facilities (shops, banks, churches, museums) on the Dolná Street and SNP Square in Banská Bystrica (Slovakia) during the coronavirus pandemic. This phenomenon is examined in the context of the pandemic situation caused by the Coronavirus, as well as in the context of the current European gender policy and the application of gender-inclusive language in Slovakia. For the sake of comparison, attention is also paid to hygiene notices in Munich (Germany).
A vese védelmében és azon is túl. A renoprotekció új lehetőségei
Authors: Balogh Dóra Bianka , Fekete Andrea , Reusz György
Published: May 2023

Az utóbbi évtized kutatásai és klinikai vizsgálatai eredményeképpen a renoprotekció új hatásmechanizmusú gyógyszerei kerültek be a klinikai gyakorlatba. A már ” aranystandardnak” tartott, a renin-angiotenzin rendszer gátlásával ható gyógyszereket követően olyan új, eredetileg a diabetes mellitus kezelésére bevezetett gyógyszerek bővítették a terápiás palettánkat, mint a glükagonszerű peptid-1-receptor-agonisták (GLP-1RA), amelyek diabeteses nephropathiában csökkentik a tartós macroalbuminuriát és a szérumkreatinin-szintet, valamint a nátrium-glükóz kotranszporter-2 (SGLT-2) -gátlók, amelyekre diabeteses nephropathián túl, a nem cukorbetegség okozta vesekárosodás terápiájára is rendelkezünk már evidenciával. Jól tervezett klinikai vizsgálatok igazolták e szereknek szív és érrendszeri protektív hatását is, ezzel összefüggésben számos igazolt, illetve potenciális mechanizmust írtak le, amelyek a fent leírt hatásokban szerepet játszhatnak. Különösen ígéretes az a megfigyelés, hogy az SGLT-2-gátlók esetén az eredetileg diabetesben igazolt nefroprotekció a nem diabeteses, krónikus vesebetegekben is érvényesül, ezzel új terápiás eszközt biztosítva a krónikus vesebetegség progressziójának lassítására. A továbbiakban e gyógyszerek hatásmechanizmusának még részletesebb feltárására van szükség, valamint arra, hogy a gyakorlati helyüket és eredményességüket, populációs vizsgálatokban, a ” mindennapi életben” is igazolni tudjuk.
Is Interstitial Macrophage Mainly Responsible for Lung Injury in SARS-CoV-2 Infection?
Authors: López José Guillermo Cabanillas
Published: May 2023

The course of the COVID-19 pandemic has led to high mortality rates worldwide, which justifies the development of various research studies aimed at elucidating the physiopathological mechanisms involved in the development of lung injury associated with this disease. The angiotensin-converting enzyme 2 (ACE2) receptor plays a leading role as the viral anchoring point necessary for viral replication to begin, so a thorough understanding of the regulatory mechanisms of this receptor is vital. Similarly, the distribution of ACE2 will justify the injury caused by SARS-CoV-2. Macrophages play a more significant role in lung injury since they allow the SARS-CoV-2 virus to reach tissues lacking ACE2 receptors and cause significant tissue damage. Therefore, all factors that influence macrophage migration and mobilization will be considered risk factors for the development of severe lung injury in COVID-19.
In silico Analysis for the Repurposing of Broad-spectrum Antiviral Drugs against Multiple Targets from SARS-CoV-2: A Molecular Docking and ADMET Approach
Authors: Parihar Arpana , Zafar Tabassum , Khandia Rekha , Parihar Dipesh Singh , Dhote Rupali , Mishra Yogesh , Khan Raju
Published: May 2023

Background: Amidst the second wave of COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) led the world devastated, and resulted in the death of millions of people with its deadly virulence potential. In comparison to similar virus outbreaks, such as severe acute respiratory syndrome coronavirus(SARS CoV) and middle east respiratory syndrome coronavirus (MERS CoV), COVID-19 led to severe morbidity and mortality. Various therapeutic interventions to combat the SARS-CoV-2 infection are actively investigated, but still, there is no specific drug with high anti-viral efficacy against the SARS-CoV-2 virus has been reported yet. The present work is an effort to represent the promising therapeutic efficacy of 52 broad-spectrum anti-viral drugs as a potential lead molecule to suppress SARS-CoV-2 infection. These are the drugs that have shown potential efficacy against several viral infections earlier. The present article discusses the comparative analysis of the therapeutic efficacy of available broad-spectrum anti-viral drugs via assessment of receptor-ligand interaction using the molecular docking approach.

Results: Based on the molecular docking indications, we predict the potential importance of various broad-spectrum antiviral drugs that can be repurposed for the treatment of SARS-CoV-2. Molecular docking revealed that Remedesivir, Imatinib, Herbacetin, Zanamivir, Ribavirin, Dasabuvir, Rhoifolin, Sofosbuvir, Cirsimaritin, and 2H-Cyclohepta[b]thiophene-3-carboxamide having strong interactions with respective targets.

Conclusion: The present piece of work strongly recommends the anti-viral potential of Zanamivir for RdRp enzyme inhibition, Herbacetin against receptor binding domain of spike protein, and main protease target, Adefovir for ACE2, and Ribavirin for endoribonuclease active site. The current predictions will enhance the clinical development of potential therapeutic drugs to combat the pandemic significantly.
Att arbeta i ”möjlighetsfönstret”
Authors: Brännvall Mari , Ekström Veronica
Published: May 2023

Using the window of opportunity - Co-operation between police and social services in cases of domestic violence in families with children.This article describes and discusses the cooperation between police and social services when working in accordance with the so-called Icelandic model. The aim of this article is to explore what cooperation within the Icelandic model during the “window of opportunity” means for police officers and social workers discretion and their possibility to perform their work in acute cases of domestic violence in families with children. Empirical data consists of interviews with police officers and social workers. Theories of new institutionalism and street level bureaucrats are used in the analysis.Results show that the Icelandic model has led to increased cooperation between police and social workers at the crime scene. The police and social workers state that they can focus to a greater extent on carrying out what they regard as their own tasks in acute cases of domestic violence when they work according to the model. At the same time, the model has entailed increased control of their work, which has reduced the degree of discretion and possibility for professional assessments and priorities. The perception that there is a “window of opportunity” is shared by the police and the social services and they believe that coordinated efforts in the acute phase are important.
Ny avhandling: Rebecka Strandell (2022)
Authors: Dunér Anna
Published: May 2023

Rebecka Strandells doktorsavhandling i socialt arbete, Omsorgsarbete i hemtjänsten: förändringar, utmaningar och möjligheter, är en sammanläggningsavhandling som inkluderar fyra delarbeten och en sammanbindande kappa. Som tidigare enhetschef i hemtjänsten vet jag att baspersonalens arbete redan på 1990-talet alltmer präglades av korta punktinsatser hos hjälptagarna och ökande tidspress. Sedan dess har stora omstruktureringar skett inom äldreomsorgen: förskjutning från äldreboende till hemtjänst, striktare biståndsbedömning samt ökat inslag av privata aktörer i och med introduktionen av kundval. Hemtjänsten har därmed kommit att i allt högre grad koncentreras till äldre personer med omfattande och komplexa hjälpbehov. Dessutom har resurserna till äldreomsorgen minskat i förhållande till andelen äldre i befolkningen. Det var därför med spänning, men också med viss oro, som jag började läsa avhandlingen.
Uma revisão sistemática sobre a psicomotricidade e as dificuldades de aprendizagem
Authors: Lordani Silvia Fernanda de Souza , Blanco Marília Bazan
Published: May 2023

O presente estudo visa identificar, por meio de uma revisão sistemática de literatura, o que tem sido produzido no Brasil referente à temática Psicomotricidade e Dificuldades de Aprendizagem. Com o objetivo de encontrar as principais pesquisas relacionadas à referida temática, apontando seus objetivos e apresentando a sí­ntese dos resultados coletados, esta revisão partiu da busca no Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal de Ní­vel Superior (CAPES), na Biblioteca Digital Brasileira de Teses e Dissertações do Instituto Brasileiro de Informação em Ciência e Tecnologia (IBICT) e na Biblioteca Virtual em Saúde (BIREME - LILACS), nos quais, após seleção, encontrou-se disponí­veis online para análise vinte e dois (22) trabalhos abordando a temática de interesse. Selecionadas as produções cientí­ficas, realizou-se um estudo dos oito (8) artigos, das doze (12) dissertações e das duas (2) teses encontradas para análise de acordo com a temática de interesse do estudo. Iniciou-se, então, uma análise e discussão mais aprofundada das pesquisas selecionadas. Como resultado, aponta-se que os trabalhos abordam, principalmente, a relação existente entre os elementos psicomotores, desempenho escolar e dificuldades de aprendizagem em crianças em idade escolar, prevalecendo às que correspondem a Educação Infantil. Evidencia-se a necessidade de aprofundar estudos nesta área, já que a presente revisão revelou que a psicomotricidade, estimulada desde a Educação Infantil, poderá prevenir dificuldades de aprendizagem em escolares, por outro lado, o estudo ainda revelou uma grande lacuna na formação de professores em relação à psicomotricidade.
Functionalised-biomatrix for wound healing and cutaneous regeneration: future impactful medical products in clinical translation and precision medicine
Authors: Fadilah Nur Izzah Md , Riha Shaima Maliha , Mazlan Zawani , Wen Adzim Poh Yuen , Hao Looi Qi , Joseph Blessy , Maarof Manira , Thomas Sabu , Motta Antonella , Fauzi Mh Busra
Published: May 2023

Skin tissue engineering possesses great promise in providing successful wound injury and tissue loss treatments that current methods cannot treat or achieve a satisfactory clinical outcome. A major field direction is exploring bioscaffolds with multifunctional properties to enhance biological performance and expedite complex skin tissue regeneration. Multifunctional bioscaffolds are three-dimensional (3D) constructs manufactured from natural and synthetic biomaterials using cutting-edge tissue fabrication techniques incorporated with cells, growth factors, secretomes, antibacterial compounds, and bioactive molecules. It offers a physical, chemical, and biological environment with a biomimetic framework to direct cells toward higher-order tissue regeneration during wound healing. Multifunctional bioscaffolds are a promising possibility for skin regeneration because of the variety of structures they provide and the capacity to customise the chemistry of their surfaces, which allows for the regulated distribution of bioactive chemicals or cells. Meanwhile, the current gap is through advanced fabrication techniques such as computational designing, electrospinning, and 3D bioprinting to fabricate multifunctional scaffolds with long-term safety. This review stipulates the wound healing processes used by commercially available engineered skin replacements (ESS), highlighting the demand for a multifunctional, and next-generation ESS replacement as the goals and significance study in tissue engineering and regenerative medicine (TERM). This work also scrutinise the use of multifunctional bioscaffolds in wound healing applications, demonstrating successful biological performance in the in vitro and in vivo animal models. Further, we also provided a comprehensive review in requiring new viewpoints and technological innovations for the clinical application of multifunctional bioscaffolds for wound healing that have been found in the literature in the last 5 years.
Reversal of memory and autism-related phenotypes in Tsc2+/− mice via inhibition of Nlgn1
Authors: Chalkiadaki Kleanthi , Statoulla Elpida , Zafeiri Maria , Haji Nabila , Lacaille Jean-Claude , Powell Craig M. , Jafarnejad Seyed Mehdi , Khoutorsky Arkady , Gkogkas Christos G.
Published: May 2023

Tuberous sclerosis complex (TSC) is a rare monogenic disorder co-diagnosed with high rates of autism and is caused by loss of function mutations in the TSC1 or TSC2 genes. A key pathway hyperactivated in TSC is the mammalian/mechanistic target of rapamycin complex 1 (mTORC1), which regulates cap-dependent mRNA translation. We previously demonstrated that exaggerated cap-dependent translation leads to autism-related phenotypes and increased mRNA translation and protein expression of Neuroligin 1 (Nlgn1) in mice. Inhibition of Nlgn1 expression reversed social behavior deficits in mice with increased cap-dependent translation. Herein, we report elevated translation of Nlgn1 mRNA and an increase in its protein expression. Genetic or pharmacological inhibition of Nlgn1 expression in Tsc2+/− mice rescued impaired hippocampal mGluR-LTD, contextual discrimination and social behavior deficits in Tsc2+/− mice, without correcting mTORC1 hyperactivation. Thus, we demonstrate that reduction of Nlgn1 expression in Tsc2+/− mice is a new therapeutic strategy for TSC and potentially other neurodevelopmental disorders.
A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application
Authors: Jia Nan , Wang Chaohong , Liu Xiaming , Huang Xiaolan , Xiao Fei , Fu Jin , Sun Chunrong , Xu Zheng , Wang Guirong , Zhou Juan , Wang Yi
Published: May 2023

Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the second leading cause of death after COVID-19 pandemic. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a novel detection platform for tuberculosis diagnosis, termed MTB-MCDA-CRISPR. MTB-MCDA-CRISPR pre-amplified the specific sdaA gene of MTB by MCDA, and the MCDA results were then decoded by CRISPR-Cas12a-based detection, resulting in simple visual fluorescent signal readouts. A set of standard MCDA primers, an engineered CP1 primer, a quenched fluorescent ssDNA reporter, and a gRNA were designed targeting the sdaA gene of MTB. The optimal temperature for MCDA pre-amplification is 67°C. The whole experiment process can be completed within one hour, including sputum rapid genomic DNA extraction (15 minutes), MCDA reaction (40 minutes), and CRISPR-Cas12a-gRNA biosensing process (5 minutes). The limit of detection (LoD) of the MTB-MCDA-CRISPR assay is 40 fg per reaction. The MTB-MCDA-CRISPR assay does not cross reaction with non-tuberculosis mycobacterium (NTM) strains and other species, validating its specificity. The clinical performance of MTB-MCDA-CRISPR assay was higher than that of the sputum smear microscopy test and comparable to that of Xpert method. In summary, the MTB-MCDA-CRISPR assay is a promising and effective tool for tuberculosis infection diagnosis, surveillance and prevention, especially for point-of-care (POC) test and field deployment in source-limited regions.
Has building innovative provinces reduce environmental pollution?--evidence from a quasi-natural experiment in China
Authors: Tang Lu , Tang Mengcong , Xi Wenjia
Published: May 2023

The implementation of innovative provinces as a pivotal pilot measure to promote the in‐depth advancement of ecology in China is a critical undertaking. An accurate assessment of the environmental effects of these innovative provinces is necessary to obtain a comprehensive understanding of the impact of pilot policies and facilitate the promotion of future policies with precision. In this study, the difference‐in‐differences method and the mediation model are employed to analyze the effect and mechanism of the pilot policies for innovative provinces on regional environmental pollution in 30 provinces in China from 2008-2020. The results demonstrate that the innovation provincial policies have resulted in an average reduction of 14.6% in environmental pollution annually in the pilot areas. This conclusion is still valid after robustness tests are eliminated. Furthermore, the mediation effect model provides evidence that the innovation provinces pilot policy reduces environmental pollution through technological innovation and industrial structure upgrading. Additionally, the heterogeneity analysis finds that innovation provinces pilot policy have led to a reduction of 17.4% in environmental pollution in coastal regions and a reduction of 11.7% in inland regions annually. Overall, this research contributes to the existing literature by underscoring the importance of innovation‐driven development for environmental governance and effectively promoting the construction of a resource‐saving and environment‐friendly society.
Tumor-neutrophil crosstalk promotes in vitro and in vivo glioblastoma progression
Authors: Rubenich Dominique S. , de Souza Priscila O. , Omizzollo Natalia , Aubin Mariana R. , Basso Paulo J. , Silva Luisa M. , da Silva Eloisa M. , Teixeira Fernanda C. , Gentil Gabriela F.S. , Domagalski Jordana L. , Cunha Maico T. , Gadelha Kerolainy A. , Diel Leonardo F. , Gelsleichter Nicolly E. , Rubenich Aline S. , Lenz Gabriela S. , de Abreu Aline M. , Kroeff Giselle M. , Paz Ana H. , Visioli Fernanda , Lamers Marcelo L. , Wink Marcia R. , Worm Paulo V. , Araújo Anelise B. , Sévigny Jean , Câmara Niels O. S. , Ludwig Nils , Braganhol Elizandra
Published: May 2023

Introduction

The tumor microenvironment (TME) of glioblastoma (GB) is characterized by an increased infiltration of immunosuppressive cells that attenuate the antitumor immune response. The participation of neutrophils in tumor progression is still controversial and a dual role in the TME has been proposed. In this study, we show that neutrophils are reprogrammed by the tumor to ultimately promote GB progression.

Methods

Using in vitro and in vivo assays, we demonstrate the existence of bidirectional GB and neutrophil communication, directly promoting an immunosuppressive TME.

Results and discussion

Neutrophils have shown to play an important role in tumor malignancy especially in advanced 3D tumor model and Balb/c nude mice experiments, implying a time- and neutrophil concentration-dependent modulation. Studying the tumor energetic metabolism indicated a mitochondria mismatch shaping the TME secretome. The given data suggests a cytokine milieu in patients with GB that favors the recruitment of neutrophils, sustaining an anti-inflammatory profile which is associated with poor prognosis. Besides, glioma-neutrophil crosstalk has sustained a tumor prolonged activation via NETs formation, indicating the role of NFκB signaling in tumor progression. Moreover, clinical samples have indicated that neutrophil-lymphocyte ratio (NLR), IL-1β, and IL-10 are associated with poor outcomes in patients with GB.

Conclusion

These results are relevant for understanding how tumor progression occurs and how immune cells can help in this process.
The role of nanotherapy in head and neck squamous cell carcinoma by targeting tumor microenvironment
Authors: Zhang Ye , Dong Pengbo , Yang Lu
Published: May 2023

Head and neck squamous cell carcinomas (HNSCCs) refers to a group of highly malignant and pathogenically complex tumors. Traditional treatment methods include surgery, radiotherapy, and chemotherapy. However, with advancements in genetics, molecular medicine, and nanotherapy, more effective and safer treatments have been developed. Nanotherapy, in particular, has the potential to be an alternative therapeutic option for HNSCC patients, given its advantageous targeting capabilities, low toxicity and modifiability. Recent research has highlighted the important role of the tumor microenvironment (TME) in the development of HNSCC. The TME is composed of various cellular components, such as fibroblasts, vascular endothelial cells, and immune cells, as well as non-cellular agents such as cytokines, chemokines, growth factors, extracellular matrix (ECM), and extracellular vesicles (EVs). These components greatly influence the prognosis and therapeutic efficacy of HNSCC, making the TME a potential target for treatment using nanotherapy. By regulating angiogenesis, immune response, tumor metastasis and other factors, nanotherapy can potentially alleviate HNSCC symptoms. This review aims to summarize and discuss the application of nanotherapy that targets HNSCC’s TME. We highlight the therapeutic value of nanotherapy for HNSCC patients.
Reservoir classification and log prediction of gas hydrate occurrence in the Qiongdongnan Basin, South China Sea
Authors: Zhu Linqi , Zhou Xueqing , Sun Jin , Liu Yanrui , Wang Jingci , Wu Shiguo
Published: May 2023

Classifying natural gas hydrate reservoirs effectively and carrying out reservoir classification modelling is crucial, but to date, research on building artificial intelligence-assisted logging curve reservoir classification models is not abundant. As exploration and development have progressed, an increasing number of fine-grained reservoirs are being discovered, and their strong heterogeneity makes correct reservoir classification even more important. Two wells used for detecting hydrates in the Qiongdongnan (QDN) Basin are used to explore the relationship between logging response parameters and reservoir quality, as well as the method of building a logging-based reservoir classification model. Through K-means clustering and Adaboost methods, the K-means method is considered to be able to correspond to the hydrate enrichment degree, while the random forest method can establish an effective reservoir classification model (the recognition accuracy is 95%). In the different categories of reservoirs, the physical properties of the reservoirs are obviously poor, and the corresponding hydrate saturation is also low, which indicates that heterogeneity has indeed affected the enrichment of hydrates in fine-grained reservoirs. This reservoir classification research method can effectively recognize reservoirs.
Advanced HCC with amplified mesenchymal epithelial transition factor receptor responds well to savolitinib: a case report
Authors: Yan Ningning , Zhang Ziheng , Guo Sanxing , Shen Shujing , Li Xingya
Published: May 2023

Objective

Current treatment agents for HCC are mostly immune checkpoint inhibitors (ICIs) plus bevacizumab and multitarget tyrosine kinase inhibitors (TKIs); however, their limited overall response rate and shorter median progression-free survival (PFS) discourage their frequent use. The development of Mesenchymal Epithelial Transition Factor receptor (MET) Tyrosine Kinase Inhibitors (MET-TKI) has transformed the treatment pattern in MET-altered solid tumors and improved their prognosis. However, the benefits of MET-TKIs in MET-amplified hepatocellular carcinoma (HCC) remain unclear.

Methods

Here, we present a case of advanced HCC amplified with MET treated with savolitinib, a MET-TKI, after progression from first-line treatment with bevacizumab plus sintilimab.

Results

The patient achieved a partial response (PR) to savolitinib in the second line setting. The progression-free survival (PFS) of first-line of bevacizumab plus sintilimab and sequential second-line treatment with MET-TKI, savolitinib, are 3 and over 8 months, respectively. furthermore, the patient still had continuous PR status with manageable toxicities.

Conclusions

The present case report provides first-hand evidence that savolitinib may be beneficial for patients with advanced MET-amplified HCC and offers a promising treatment option.
Use of cell-free signals as biomarkers for early and easy prediction of preeclampsia
Authors: Gekas Jean , Boomer Theresa Hopkins , Rodrigue Marc-André , Jinnett Kristine N. , Bhatt Sucheta
Published: May 2023

Introduction

Preeclampsia (PE) is a leading cause of maternal and perinatal morbidity worldwide. However, current methods of screening are complicated and require special skill sets. In this observational study of prospectively collected samples, we wanted to evaluate if cell-free (cf) DNA could be an efficient biomarker for identification of at-risk patients.

Methods

One hundred patients attending a private prenatal clinic in Canada were enrolled in their first trimester of pregnancy and a blood draw was carried out at 11 + 0 to 14 + 2 weeks’ (timepoint A) and 17 + 6 to 25 + 5 weeks of gestation (timepoint B). CfDNA signals, namely concentration, fetal fraction, and fragment size distribution, were correlated with clinical outcomes in the test population to develop the logistic regression model.

Results

Twelve patients developed PE—four early-stage and eight late-stage PE. Significant differences were observed between PE patients and control cases for all three cfDNA signals at timepoint A, while both fetal fraction and concentration were significantly different between PE patients and control cases at timepoint B. Overall, the model had a sensitivity of up to 100% and specificity of up to 87.5% at Timepoint A.

Conclusion

This proof-of-principle study showed that use of this logistic regression model could identify patients at risk of preeclampsia in the first trimester of pregnancy.
Case report: COVID-19-associated refractory thrombotic thrombocytopenic purpura complicated with Guillain-Barré syndrome
Authors: Zhou Rui , Chen Shenjian , Luo Qinghua , Zhang Xinyue , Li Fang , Huang Wei , Sheng Zhiyong
Published: May 2023

Thrombotic thrombocytopenic purpura (TTP), a rare and lethal thrombotic microangiopathy, is an autoimmune disease that can be triggered by viral infections such as COVID-19. This condition is characterized by hemolytic microangiopathy, thrombocytopenia, and neurologic alterations, possibly accompanied by fever and renal damage. Moreover, more than 220 patients with Guillain-Barré syndrome (GBS) have been reported in association with the COVID-19 infection. In this report, we present a case of a patient who developed refractory TTP complicated by GBS following a SARS-CoV-2 infection. We aimed to highlight the importance of accurately diagnosing neurological complications associated with a COVID-19 infection and to demonstrate our strategies for treating a patient with COVID-19 infection-related refractory TTP complicated by GBS.
Hyper-parameter tuning and feature extraction for asynchronous action detection from sub-thalamic nucleus local field potentials
Authors: Martineau Thomas , He Shenghong , Vaidyanathan Ravi , Tan Huiling
Published: May 2023

Introduction

Decoding brain states from subcortical local field potentials (LFPs) indicative of activities such as voluntary movement, tremor, or sleep stages, holds significant potential in treating neurodegenerative disorders and offers new paradigms in brain-computer interface (BCI). Identified states can serve as control signals in coupled human-machine systems, e.g., to regulate deep brain stimulation (DBS) therapy or control prosthetic limbs. However, the behavior, performance, and efficiency of LFP decoders depend on an array of design and calibration settings encapsulated into a single set of hyper-parameters. Although methods exist to tune hyper-parameters automatically, decoders are typically found through exhaustive trial-and-error, manual search, and intuitive experience.

Methods

This study introduces a Bayesian optimization (BO) approach to hyper-parameter tuning, applicable through feature extraction, channel selection, classification, and stage transition stages of the entire decoding pipeline. The optimization method is compared with five real-time feature extraction methods paired with four classifiers to decode voluntary movement asynchronously based on LFPs recorded with DBS electrodes implanted in the subthalamic nucleus of Parkinson’s disease patients.

Results

Detection performance, measured as the geometric mean between classifier specificity and sensitivity, is automatically optimized. BO demonstrates improved decoding performance from initial parameter setting across all methods. The best decoders achieve a maximum performance of 0.74 ± 0.06 (mean ± SD across all participants) sensitivity-specificity geometric mean. In addition, parameter relevance is determined using the BO surrogate models.

Discussion

Hyper-parameters tend to be sub-optimally fixed across different users rather than individually adjusted or even specifically set for a decoding task. The relevance of each parameter to the optimization problem and comparisons between algorithms can also be difficult to track with the evolution of the decoding problem. We believe that the proposed decoding pipeline and BO approach is a promising solution to such challenges surrounding hyper-parameter tuning and that the study’s findings can inform future design iterations of neural decoders for adaptive DBS and BCI.
Acute psilocybin increased cortical activities in rats
Authors: Liu Junhong , Wang Yuanyuan , Xia Ke , Wu Jinfeng , Zheng Danhao , Cai Aoling , Yan Haitao , Su Ruibin
Published: May 2023

Psilocybin, a naturally occurring hallucinogenic component of magic mushrooms, has significant psychoactive effects in both humans and rodents. But the underlying mechanisms are not fully understood. Blood-oxygenation level-dependent (BOLD) functional magnetic resonance imaging (fMRI) is a useful tool in many preclinical and clinical trials to investigate psilocybin-induced changes of brain activity and functional connectivity (FC) due to its noninvasive nature and widespread availability. However, fMRI effects of psilocybin on rats have not been carefully investigated. This study aimed to explore how psilocybin affects resting-state brain activity and FC, through a combination of BOLD fMRI and immunofluorescence (IF) of EGR1, an immediate early gene (IEG) closely related to depressive symptoms. Ten minutes after psilocybin hydrochloride injection (2.0 mg/kg, i.p.), positive brain activities were observed in the frontal, temporal, and parietal cortex (including the cingulate cortex and retrosplenial cortex), hippocampus, and striatum. And a region-of-interest (ROI) -wise FC analysis matrix suggested increased interconnectivity of several regions, such as the cingulate cortex, dorsal striatum, prelimbic, and limbic regions. Further seed-based analyses revealed increased FC of cingulate cortex within the cortical and striatal areas. Consistently, acute psilocybin increased the EGR1 level throughout the brain, indicating a consistent activation thought the cortical and striatal areas. In conclusion, the psilocybin-induced hyperactive state of rats is congruent to that of humans, and may be responsible for its pharmacological effects.
Visualizing the triheteromeric N-methyl-D-aspartate receptor subunit composition
Authors: Beesley Stephen , Gunjan Akash , Kumar Sanjay S.
Published: May 2023

N-methyl-D-aspartate receptors (NMDARs) are one of three ligand-gated ionotropic channels that transduce the effects of neurotransmitter glutamate at excitatory synapses within the central nervous system. Their ability to influx Ca2+ into cells, unlike mature AMPA or kainate receptors, implicates them in a variety of processes ranging from synaptic plasticity to cell death. Many of the receptor’s capabilities, including binding glutamate and regulating Ca2+ influx, have been attributed to their subunit composition, determined putatively using cell biology, electrophysiology and/or pharmacology. Here, we show that subunit composition of synaptic NMDARs can also be readily visualized in acute brain slices (rat) using highly specific antibodies directed against extracellular epitopes of the subunit proteins and high-resolution confocal microscopy. This has helped confirm the expression of triheteromeric t-NMDARs (containing GluN1, GluN2, and GluN3 subunits) at synapses for the first time and reconcile functional differences with diheteromeric d-NMDARs (containing GluN1 and GluN2 subunits) described previously. Even though structural information about individual receptors is still diffraction limited, fluorescently tagged receptor subunit puncta coalesce with precision at various magnifications and/or with the postsynaptic density (PSD-95) but not the presynaptic active zone marker Bassoon. These data are particularly relevant for identifying GluN3A-containing t-NMDARs that are highly Ca2+ permeable and whose expression at excitatory synapses renders neurons vulnerable to excitotoxicity and cell death. Imaging NMDAR subunit proteins at synapses not only offers firsthand insights into subunit composition to correlate function but may also help identify zones of vulnerability within brain structures underlying neurodegenerative diseases like Temporal Lobe Epilepsy.