bluuebunny/crossref_metadata_2025 huggingface dataset:
Effectiveness of Transcranial Direct Current Stimulation in Behavioral and Cognitive Aspects of Executive Function in Children with Autism Spectrum Disorder
Authors: Amiri Maryam Alsadat , Karamali Esmaili Samaneh , Saei Shafagh , Alizadeh Zarei Mehdi
Published: November 2022

Background: Despite their high effectiveness, rehabilitation therapies can reduce the number of families with children with autism spectrum disorder (ASD) visiting clinics due to the number of treatment sessions and the long duration of treatment. The use of technological methods, such as transcranial direct current stimulation (tDCS) as one of the therapeutic interventions to improve cognitive function in rehabilitation clinics, can help reduce the duration of treatment for these children. Objectives: The present study was carried out to examine the effectiveness of tDCS in behavioral and cognitive aspects of executive functions in children with ASD. Methods: A pretest and posttest design with follow-up were used in this study. The subjects were children with high-functioning ASD aged 8 - 11 years who were selected by the convenience sampling method from occupational therapy clinics in Tehran, Iran. A total of 20 eligible candidates were included in the study according to inclusion and exclusion criteria. The subjects were randomly divided into two groups (10 subjects in each group). The intervention group received tDCS during 12 sessions of 20 minutes in the dorsolateral prefrontal cortex area, with an electrode size of 35 cm2 for 3 weeks (four sessions per week). The control group received common cognitive interventions used in occupational therapy clinics in 12 sessions of 60 minutes (one hour) for 6 weeks (two sessions per week). Follow-up was performed one month after the end of the interventions. The research instruments included the Behavior Rating Inventory of Executive Function and computer-based tests, such as the Tower of London, Wisconsin Card Sorting Test, and Stroop test. Results: The results demonstrated that tDCS, similar to cognitive rehabilitation, could affect the cognitive functions of the brain, such as executive functions, and several basic cognitive processes, such as inhibitory control, cognitive flexibility, and mental planning. Conclusions: The findings recommended using tDCS as a new intervention method to improve the cognitive function of individuals with ASD. This is due to the lasting effect of the results of this intervention as a technological instrument with faster results than other clinical interventions.
Identifying the Trends of Global Publications in Health Information Technology Using Text-mining Techniques
Authors: Dastani Meisam , Ehtesham Hamideh , Javanmard Zohreh , Sabahi Azam , Bahador Fateme
Published: November 2022

Background: Due to the increased publication of articles in various scientific fields, analyzing the published topics in specialized journals is important and necessary. Objectives: This research has identified the published topics in global publications in the health information technology (HIT) field. Methods: This study analyzed articles in the field of HIT using text-mining techniques. For this purpose, 162,994 documents were extracted from PubMed and Scopus databases from 2000 to 2019 using the appropriate search strategy. Text mining techniques and the Latent Dirichlet Allocation (LDA) topic modeling algorithm were used to identify the published topics. Python programming language has also been used to run text-mining algorithms. Results: This study categorized the subject of HIT-related published articles into 16 topics, the most important of which were Telemedicine and telehealth, Adoption of HIT, Radiotherapy planning techniques, Medical image analysis, and Evidence-based medicine. Conclusions: The results of the trends of subjects of HIT-related published articles represented the thematic extent and the interdisciplinary nature of this field. The publication of various topics in this scientific field has shown a growing trend in recent years.
Traducción y adaptación transcultural de la Escala “Patient Specific Functional Scale”
Authors: Depine Julieta Marina , Perrone Marelo Lucas , Melia Marianela , Paredes Andrea Lorena , Errico María Laura , Giangarrá Alejandro , Bianco Matías , Ottaviani Franco , Guardia Gonzalo Matías , Rubio Mariana Gabriela , Stivanello Santiago
Published: November 2022

Objetivo: Traducción y adaptación transcultural al español de Argentina de la Patient Specific Functional Scale (PSFS).Materiales y método: El proceso de traducción consistió en cinco etapas que abarcaron desde la traducción de la versión original, síntesis y retrotraducción hasta el desarrollo de una versión pre-final, cuya comprensión fue evaluada por pacientes que concurrieron a la Unidad de Kinesiología del Hospital General de Agudos “Dr. Ignacio Pirovano”.Resultados: Se entrevistaron 31 sujetos, siendo 16 (51,6 %) mujeres, con una mediana de edad de 56 años.Con respecto a la impresión general y a la comprensión de las consignas, el 96,8 % de los sujetos respondió de forma satisfactoria a las preguntas.Conclusión: Hemos obtenido con éxito una versión final en español de la PSFS con la finalidad de poder serutilizada en el ámbito de la rehabilitación.
Primera encuesta online sobre el abordaje kinésico de las afecciones de hombro en Argentina
Authors: Policastro Pablo , Navarro Emiliano , Novoa Gabriel Adrian
Published: November 2022

Objetivo: Describir la práctica habitual de los kinesiólogos durante el abordaje de las afecciones de hombro en Argentina.Materiales y método: Se realizó un estudio descriptivo transversal de tipo encuesta entre noviembre y diciembre de 2016. Se incluyeron kinesiólogos que trabajan en el ámbito argentino con pacientes con afecciones musculoesqueléticas de hombro. Se los invitó a participar de forma voluntaria y anónima, enviando la encuesta mediante la herramienta Survey Monkey®. La encuesta, compuesta por 23 preguntas, fue confeccionada por dos kinesiólogos involucrados en el tratamiento de dichos pacientes.Resultados: Se analizaron en total 301 (93,5%) encuestas. En la evaluación, el 69,4% mide el rango de movimiento analítico, el 72,7% evalúa la fuerza muscular, el 65,1% utiliza test clínicos diagnósticos de patologías de manguito rotador y el 80,1% evalúa la presencia de diskinesia escapular. En el tratamiento, el 71,4% aplica distintos tipos de agentes físicos, mientras que el 71,5% combina terapia manual y ejercicios.Conclusión: Se conocieron datos relacionados con el abordaje del hombro en kinesiólogos argentinos. La mayoría de los encuestados evalúa el rango de movimiento, la diskinesia escapular y/o la fuerza muscular. Las intervenciones más utilizadas fueron la fisioterapia y el ejercicio asociado a la terapia manual. Los participantes realizan un abordaje que concuerda parcialmente con la evidencia.
Hipoxemia refractaria severa en edema por reexpansión pulmonar durante el postoperatorio de cirugía cardíaca.
Authors: Schwerdt Clarisa , Ciarlantini Agustina , Dorado Javier Hernán , Navarro Emiliano
Published: November 2022

Introducción: El edema por reexpansion pulmonar (ERP) ocurre debido a una rápida expansión pulmonar posterior al drenaje brusco de un derrame pleural de gran volumen. En casos severos puede desarrollarse hipoxemia refractaria. La ventilación pulmonar independiente y el posicionamiento son algunas de las estrategias propuestas. Debido a la similitud en su fisiopatología con el síndrome de distrés respiratorio agudo (SDRA). es posible que las intervenciones descritas para el sostén de pacientes con SDRA puedan resultar de utilidad. Sin embargo no hemos podido encontrar recomendaciones sobre su utilización en estos casos. El objetivo de esta presentación es describir las estrategias implementadas y los resultados obtenidos en un caso con ERP e hipoxemia refractaria.

Presentación del caso: Joven de 24 años, que desarrolló ERP e hipoxemia refractaria durante el posoperatorio de cirugía de reemplazo valvular aórtico por un drenaje de un hemotórax masivo derecho. Se implementó una estrategia de ventilación protectora, monitorización de la mecánica pulmonar avanzada y posición prona. El paciente evolucionó favorablemente y el día 8 se logró extubar.

Conclusión: Los resultados obtenidos permiten valorar la estrategia implementada como una alternativa a la ventilación pulmonar independiente en casos severos.
Epidemiología de sujetos con afecciones músculo-esqueléticas que acuden a un consultorio privado de kinesiología en Argentina
Authors: Pierobon Andrés , Policastro Pablo , Ponce Amorín Maximiliano , Navarro Emiliano , Mendez Diego , Raguzzi Ignacio
Published: November 2022

Objetivo: Describir las características de los sujetos que acuden a un consultorio privado de kinesiología en Argentina. Como objetivo secundario, evaluar la relación entre el Índice de masa corporal (IMC) y las regiones corporales afectadas.

Materiales y método: Se realizó un estudio retrospectivo donde se incluyeron sujetos que acudieron a un consultorio privado de kinesiología de Argentina. Se excluyeron los sujetos con ausencia de datos sobre la región corporal afectada. Se recolectaron variables sociodemográficas y variables relacionadas con la lesión. Registro en clinicaltrials.gov: NCT04350242.

Resultados: Se analizaron los datos de 1.539 sujetos. El 61,8% fueron hombres con una mediana de edad de 37 años. La rodilla, el hombro y la zona de tobillo y pie fueron las 3 regiones corporales más prevalentes con el 21,1%, 16,3% y 14,4%, respectivamente. Se encontró una asociación positiva entre el IMC y la probabilidad de presentarse con una afección de rodilla (OR= 1,04; IC 95% 1,01 - 1,07). Cada dos puntos de aumento en el IMC las chances de presentarse con una lesión en la rodilla se incrementaron un 16%. Los sujetos con dolor de columna tuvieron mayor duración de los síntomas que los sujetos con afecciones en otras regiones (p< 0,001).

Conclusión: El presente estudio nos permitió conocer que la mayoría de los sujetos que acudieron a un centro de kinesiología privada fueron hombres entre 30 y 40 años, que las afecciones de rodilla fueron las más prevalentes y que las mismas se asociaron de manera positiva con el IMC.
Uso del asistente mecánico de la tos en sujetos neuromusculares pediátricos en el domicilio
Authors: Mozzoni Julieta Luján , Gómez Soledad
Published: November 2022

Objetivo: Describir las características del uso del asistente mecánico de la tos (AMT) en el domicilio en niños/as con enfermedades neuromusculares (ENM) en seguimiento en nuestro hospital.

Materiales y método: Estudio observacional, descriptivo, transversal y retrospectivo. Se incluyeron todos los sujetos pediátricos con ENM con AMT domiciliario con un uso del mismo no menor a seis meses, entre el 1 de enero de 2018 y el 31 de abril de 2019.

Resultados: Fueron incluidos 59 sujetos, con una media de edad en años de 8,84 (± 4,01). El diagnóstico más frecuente fue Atrofia Muscular Espinal (AME) tipo II en 32 casos (54,2%). La mediana de edad al inicio del uso del AMT fue de 4 años (RIQ 3-7). El principal motivo de su indicación fueron las intercurrencias respiratorias a repetición. El equipo más utilizado fue el Philips Respironics E70® , en modo automático, con presiones de 40 cmH2O positiva y negativa, y la interfase más frecuente, la máscara oronasal. No se reportaron complicaciones.

Conclusión: El diagnóstico más prevalente de los sujetos pediátricos con uso domiciliario del AMT fue AME, y el mismo se indicó mayormente por cuadros respiratorios a repetición. Los principales usuarios fueron los padres/ madres, en modo automático y con presiones de ±40 cmH2O.
Comentario a Policastro PO et al., “Primera encuesta online sobre el abordaje kinésico de las afecciones de hombro en Argentina”
Authors: AJRPT AJRPT , Muhafara Gastón
Published: November 2022

No disponible.
Evolución de la ventilación mecánica, traqueostomía y rehabilitación de un paciente con COVID-19, en un hospital público argentino
Authors: Castro Daniela Rocío
Published: November 2022

Introducción: La pandemia de la enfermedad por coronavirus de 2019 (COVID-19) se ha desarrollado rápidamente y desafía los sistemas de salud y a la sociedad. Se describe el primer paciente con COVID-19 con ventilación mecánica (VM) prolongada y traqueostomía (TQT) en la unidad de cuidados intensivos (UCI) de nuestro hospital.Presentación del caso: Paciente masculino de 42 años, con COVID-19 que desarrolló insuficiencia respiratoria aguda, requirió intubación orotraqueal y VM. Por sospecha de destete prolongado se le realizó TQT, posteriormente logró ser desvinculado de la VM y decanulado. Luego de 50 días de internación en UCI, evolucionó favorablemente y fue dado de alta.Conclusión: Este fue el primer paciente con COVID-19 que requirió VM y TQT en nuestro hospital. Los protocolos de VM, decanulación y rehabilitación pudieron adaptarse y ser llevados a cabo. Egresó decanulado y sin polineuropatía.
Enfermedad de Charcot Marie Tooth tipo 1A con parálisis diafragmática y alteración en el control ventilatorio
Authors: Escobar Miguel Antonio , Navarro Emiliano , Oses Carolina Mónica , González Romina Noelia , Rositi Emilio Sebastian , Morel Vulliez Gastón Germán , De Vito Eduardo Luis
Published: November 2022

Introducción: la enfermedad de Charcot-Marie-Tooth tipo 1A es la neuropatía sensitivo-motora hereditaria más frecuente. El presente reporte de caso tiene como objetivo describir la evaluación, la evolución y el tratamiento de un paciente con hipercapnia y anomalías del control ventilatorio central.

Presentación del caso: masculino de 42 años que por deterioro del sensorio requirió intubación orotraqueal post-cirugía de fractura de muñeca. Presentó hipercapnia post-extubación, por lo que se aplicó ventilación no invasiva (VNI). Al ingreso en nuestro centro presentó ortopnea, respiración paradojal en decúbito dorsal e hipercapnia. Se constató parálisis diafragmática con presión transdiafragmática máxima de 1,5 cmH2O, presión inspiratoria máxima del 39% y capacidad vital forzada del 32% de sus valores predichos. Debido a que la hipercapnia no pudo ser del todo justificada por el examen funcional respiratorio, realizamos un test de reinhalación de CO2 para evaluar el control ventilatorio. Se obtuvo una pendiente de 0,10 cmH2O/mmHg (valor debajo del normal 0,18 cmH2O/mmHg, RIQ 0,15-0,23 cmH2O/mmHg). Al día 16 fue dado de alta al domicilio con VNI nocturna.

Conclusión: las anomalías centrales del control ventilatorio deberían ser exploradas cuando la restricción y/o debilidad muscular respiratoria no son suficientemente severas como para justificar la presencia de hipercapnia.
Evaluaciones físicas en la práctica clínica kinésica
Authors: Tondelli Eduardo
Published: November 2022

En la fase final del proceso de rehabilitación de una lesión deportiva, los kinesiólogos somos los encargados del proceso de readaptación, asistiendo al deportista a recuperar los niveles perdidos de fuerza, resistencia aeróbica, velocidad, agilidad, saltos, entre otros. Para ello y para poder establecer criterios funcionales de alta, es necesario basarse en datos objetivos. El objetivo de este paso a paso es describir algunas herramientas para evaluar el rendimiento, cuáles son los objetivos de cada una, sus procedimientos y la correcta valoración de los datos obtenidos.
Validación de la versión argentina de la escala Patient Specific Functional Scale en sujetos con trastornos musculoesqueléticos
Authors: Rubio Mariana , Espiño Laura , Criscione Camila , Rodríguez Calvo Sebastián , Pirovani Mercedes , Goldar Daniela , Stivanello Santiago , Cancino Antonella , Lisazú Juan Ignacio , Cristiani Noelia , Ottaviani Franco , Guardia Gonzalo
Published: November 2022

Objetivo: Validar la versión argentina de la escala Patient Specific Functional Scale (PSFS) para trastornos musculoesqueléticos de miembro superior (MS) e inferior (MI) calculando confiabilidad test-retest, validez y sensibilidad al cambio.

Materiales y método: Diseño observacional, prospectivo y longitudinal. En la evaluación inicial, se midió funcionalidad mediante la PSFS, Disabilities of the Arm, Shoulder and Hand (DASH) y/o Lower Extremity Functional Scale (LEFS). En un segundo y tercer tiempo, se administró la escala Global Rating of Change, PSFS y DASH/LEFS.

Resultados: Se reclutaron 352 sujetos entre agosto de 2017 y enero de 2020. Se analizaron 336 sujetos, 165 con patologías de MS y 171 de MI. Confiabilidad test-retest: el coeficiente de correlación intraclase (CCI) para la PSFS global fue de 0,61 (0,48 - 0,71), para MS de 0,70 (0,52 - 0,81) y para MI 0,51 (0,30  0,67). El mínimo cambio detectable (MCD) fue de 2,85, intervalo de confianza 95% (IC 95%). Para la validez de constructo, el coeficiente de correlación de Spearman fue de -0,41 para PSFS/DASH, y de 0,37 para PSFS/LEFS. En cuanto a la sensibilidad al cambio, el área bajo la curva resultó de 0,928 (IC 95% 0,874 - 0,98). Con respecto a la interpretabilidad, el mínimo cambio clínicamente significativo resultó de 2,2 puntos (n= 186), para el subgrupo de MS 2,2 (n= 86) y para el subgrupo de MI 1,71 (n= 100). 

Conclusión: La versión argentina de la PSFS presenta moderada validez de constructo, aceptable confiabilidad test-retest para MS y excelente sensibilidad al cambio.
Association between Proximity to Food Sources and Dietary Behaviors in Black and White College Graduates
Authors: Bell Caryn N. , Robles Brenda , Singleton Chelsea R. , Thomas Tobin Courtney S. , Spears Erica C. , Thorpe, Jr Roland J.
Published: November 2022

Objectives: Unequal access to healthy food environments is often implicated in racial inequities in health and behaviors that are largest among college graduates. The aim of this study was to determine associations between perceived proximity to food sources and dietary behaviors between
black and white college graduates. Methods: In a cross-sectional online survey of dietary behaviors between black and white adults who have a ≥ 4-year bachelor's degree, respondents were asked how long it typically takes for them to get to grocery stores and fast-food restaurants from home.
We used ordinal logit regression models to assess associations between perceived proximity to food sources and dietary behaviors. Results: Among black men, perceiving that a grocery store was ≥ 10 minutes from their home was associated with lower fruit consumption (beta=-0.94, SE=0.48).
Perceiving that a grocery store was ≥ 10 minutes from their home was associated with more frequent fast-food consumption among black men (beta=1.21, SE=0.39), Black women (beta=0.98, SE=0.34), and white men (beta=0.74, SE=0.30). Conclusions: The associations between perceived proximity
to food sources and dietary behaviors differ by race and sex among college graduates with important implications for racial disparities in diet quality and obesity across SES.
A Comparative Study on Visual Health and Physical Activity of Chinese and Japanese Children Aged 6-12 Years
Authors: Yang Ganfeng , Zhang Meng , Ujihara Hiroshi , Xu Jianrong , Zhou Sheng
Published: November 2022

Objectives: In this study, we compare the visual acuity levels and physical activity participation of Chinese and Japanese children. Methods: We collected data on static visual acuity, kinetic visual acuity, axial length, intensity, frequency, duration, amount, and physical activity
items of 431 children aged 6-12 years from Chinese and Japanese schools in the same city. Our analysis examined the relationships involving age, visual function indicators, ocular biometric parameters, and extracurricular physical activity. Results: We found the myopia rate higher in Chinese
children than in Japanese children, increasing with age except in grade 4; kinetic visual acuity in Chinese children was lower than in Japanese children (p<.01). The axial length becomes longer with age in both groups, with Chinese children having a longer axial length than Japanese children.
The amount of physical activity in Chinese and Japanese children aged 6-12 years fluctuates. The frequency, duration, and physical activity items are lower in Chinese children than Japanese (p<.01). Conclusions: Chinese and Japanese children's overall static visual acuity development level
shows a decreasing trend, and Japanese children have better kinetic visual acuity and physical activity participation than Chinese children. Physical activity could have an impact on visual acuity.
A Pilot Study of Virtual Reality (VR) Tai Chi Program on Mental Health among Older Adults during the COVID-19 Pandemic
Authors: Kim Junhyoung , Kim Yongseop , Chang Pei-Shiun , Min Oh Seok , Han Sua
Published: November 2022

Objectives: Tai Chi, an ancient Chinese martial art, has been credited for improving the physical and mental health of community-dwelling older adults. Virtual reality (VR) has developed as a therapeutic technique for improving health and well-being in older adults. Previous research
on exercise-based VR interventions in older adults has focused primarily on the physical and cognitive health effects. The extent to which a program integrating the ancient practice of Tai Chi with virtual reality might benefit older individuals' physical and mental health is an unknown entity.
In this pilot study, we examined potential advantages of the virtual Tai Chi program in terms of health outcomes, as well as to gather qualitative data on the barriers to VR program participation. Methods: We conducted semi-structured, in-depth interviews with 5 participants who had participated
at least 5 years in traditional Tai Chi. Results: Three key salient themes emerged as beneficial results of VR experiences: (1) mindfulness/ meditation, (2) enjoyment, and (3) physical exercise. Participants also reported 3 main challenges: (1) some difficulties with controllers and functions,
(2) cybersickness/pain, and (3) absence of group synergy. Conclusions:These challenges suggest further avenues of investigation for researchers in refining this approach.
Development of ILP-markers for Aegilops tauschii and their application in molecular genetic analysis
Authors: Hordynskyi S. O. , Postovoitova A. S. , Rabokon A. M. , Pirko Ya. V. , Blume Ya. B.
Published: November 2022

To develop marker systems based on the study of intron length polymorphism of various genes (ILP) in Ae. tauschii, to test the possibility of their use for genetic differentiation of Ae. tauschii and Ae. biuncialis. Methods. The NCBI database was used to obtain EST sequences, online tools CD-HIT, BLAST and Primer3Plus (for the development of primers). The polymerase chain reaction (PCR) method with the developed primers was done. Amplified fragments were separated by non-denaturing polyacrylamide gel electrophoresis and stained with silver. Results. Molecular markers Aet_ILP1, Aet_ILP6 were developed and tested for different genotypes of Ae. tauschii and Ae. biuncialis. No intraspecific differentiation was observed in all studied samples, but amplicons of introns of different species differed significantly. It was also found that the studied species had one copy of the HO222074.1 gene and 1 to 3 copies of the CX244643.1 gene. Conclusions. The obtained results indicate a low level of intraspecific variability of the developed ILP markers and the possibility of their use for interspecific differentiation of Ae. tauschii and Ae. biuncialis.
Nucleus-nucleolus features of certain species of the Pinaceae family
Authors: Bilonozhko Yu. O. , Korshikov I. I.
Published: November 2022

Aim: The aim of the study was to establish the interspecific features of the functioning of the nucleolus-forming organizer on the example of 6 species of the family Pinaceae. Methods. The study involved samples of different species of conifers that grow within the natural range and conditions of introduction. Silver nitric acid staining was used to visualize the nucleoli. Quantitative and dimensional features of the nucleus and nucleoli in the interphase cells of seedlings were studied, and the values of the nucleus-nucleolus ratio were calculated. Results. Interspecific polymorphism in the number and size of nucleoli, nuclei and nucleus-nucleolus ratio in six species and forms of the Pinaceae L. family was revealed. Conclusions. The studied species of conifers obviously have interspecific differentiation by nucleus-nucleolus parameters. The studied characteristics are influenced not only by the species, age and condition of the plant, but also by the natural and climatic conditions in which the population exists.
Use of mannitol for cold pretreatment of isolated spikes and as a component of nutrient medium for spring barley anther culture in vitro
Authors: Bilynska O. V.
Published: November 2022

Aim. The investigation was aimed to compare the efficiency of mannitol as a component of spike cold pretreatment solution and of medium for spring barley haploid production via anther culture in vitro. Methods. Field-grown plants of two genotypes with a contrast androgenic capacity were used as donors. Cut tillers were pretreated in water at 4⁰C for 5 days, while aseptically isolated spikes were kept in 0.3 M mannitol for 10 days at the same temperature. Isolated anthers were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose (9.0%); the media with and without 0.1M mannitol differed in solidifying agents. Results. In highly-responsive line DH00-126, there was an almost two-fold increase in mmgreen plant regeneration frequency (from 48 to 58 plants per 100 anthers) in all experimental variants in comparison to the control. In recalcitrant variety Mebere, the combination of tiller pretreatment in water with anther cultivation on inductive starch-gelling media supplemented with 0.1 M mannitol resulted in the highest green plant yield. Conclusions. Both methodical approaches were proved to be applicable to increase the efficiency of haploid production in spring barley anther culture in vitro.
Methodological aspects of increasing the intensity of callusogenesis and organogenesis of Linum usitatissimum L. in vitro
Authors: Mishchenko S. V. , Machulsky H. M.
Published: November 2022

Aim. Improving methods for increasing the efficiency of obtaining callus cultures and somaclones of flax (Linum usitatissimum L.) in vitro. Methods. Hypocotyl segments were cultured on Murashige and Skoog nutrient medium supplemented with sucrose (30 g/l) and phytohormones at various concentrations. Other conditions: photoperiod 16 hours, illuminance 2500 lx, relative humidity 60–80%, air temperature 22–24°C. Results. The ability to form callus and somatic embryogenesis of flax depends on the phytohormonal composition of the nutrient medium, the size of the explants and the distance between them. Conclusions. For intensive callus formation and somatic embryogenesis in vitro, the optimal concentrations of BAP (mg/l) can be expressed as 1.0 ≤ BAP ≤ 1.75; the optimal concentrations of BAP for the medium supplemented with NAA (0.05 mg/l) 0.5 ≤ BAP ≤ 2.0; the optimal concentration of NAA for the medium supplemented with BAP (1.0 mg/l) 0.025 ≤ NAA ≤ 0.150; and the optimal concentrations of IAA for the medium supplemented with BAP (1.0 mg/l) 0.05 ≤ IAA ≤ 0.50. Addition of 0.5 mg/l GA3 to the medium with NAA and BAP is effective. It is optimal to use hypocotyl explants 3–6 mm long and place them at a distance of 1.5–2.5 cm from each other. Organogenicity of callus is significantly reduced in the process of subculturing.
In vitro culture of Zingeria biebersteiniana (Claus) P. Smirn. as a method for conservation and restoration of genetic diversity
Authors: Twardovska M. O. , Kunakh V. A.
Published: November 2022

Aim. The aim of the work was to determine the conditions for development of in vitro culture, induction of callus formation, and long-term tissue culture of Zingeria biebersteiniana (Claus) P. Smirn. Methods. In vitro clonal propagation, tissue culture techniques. Results. The seed germination rate was found to increase significantly after long-term cold stratification. The protocol for seed sterilization was developed, which yielded 57.3% of aseptic plants. Collections of in vitro and pot cultured plants were created. Experiments on the adaptation of in vitro propagated plants to pot culture conditions revealed a high level of their survival. The optimal medium for in vitro clonal propagation was MS, supplemented with 0.1 mg/L NAA; while the most effective media for induction of callus formation and for long-term tissue culture was B5 supplemented with 2 mg/L 2,4-D and 0.1 mg/L BAP. Conclusions. The protocols and conditions for seed germination, in vitro clonal propagation, induction of callus formation, as well as long-term tissue culture of Z. biebersteiniana have been developed. The developed techniques of in vitro culture can be used for conservation and restoration of genetic diversity of the species, as well as to obtain sufficient plant material for further studies.
Evolutionary History of species-specific Alu repeats on the example of the MGMT gene of old world monkey
Authors: Pidpala O. V. , Lukash L. L.
Published: November 2022

Aim. To analyze the evolution of species-specific Alu repeats in orthologs of the MGMT gene in Cercopithecidae. Methods. The homology between nucleotide sequences has been determined by BLAST 2.6.1 program. The results of the search and identification of MGE have been realized by CENSOR program. Results. The investigation of the orthology of the MGMT gene in Old World monkeys has shown that different species-specific Alu repeats identified in intron sequences might have different evolutionary histories and dynamics of sequence degradation. An absence of species-specific Strepsirrhini Alu repeats in the MGMT gene of representatives of tarsier and New World monkeys and their selective distribution in Old World monkeys demand much more detailed phylogenetic studies.Conclusions. The evolutionary history of species-specific Alu repeats and the peculiarities of their degradation are individual.
Similarity and difference of organizations of streptomycetic landomycins clusters
Authors: Polishchuk L. V. , Lukyanchuk V. V.
Published: November 2022

Aim. The aim of the work is to establish the level of similarity of the primary structure and organization of landomycin clusters of streptomycetes. Methods. Information on nucleotide sequences and annotations of streptomycetic clusters of landomycin biosynthesis genes AF080235,1, KJ645792.1, HQ828984.1 and genomes of strains S. cyanogenus S136, S. globisporus 1912-4Crt is freely available in GenBank. Analysis of the nucleotide sequences of streptomycetes was performed using the program BLASTN from the server NSBI. Results. The remote localization of a number of probable lan-cluster genes (prx, lanW, lanYR) has been established. The sequences of similar lndU / lanU genes and lndY / lanY genes, which are not necessary for landomycin biosynthesis, have been shown to be the least similar. It is shown that the lan-cluster of S. cyanogenus S136 is expected to be localized outside the cortical region of the chromosome of the strain. Landomycin-producing streptomycetes are isolated from soil samples from different ecological niches on different continents of the planet. Conclusions. The difference in the organization of lan- and lnd-clusters is expressed in the remote localization of a number of genes (prx, lanW, lanYR).
Productivity of parental inbred lines – components of maize hybrids depending on plant density and treatment with biopreparations under conditions of drip irrigation
Authors: Vozhegova R. A. , Lavrinenko Yu. O. , Marchenko T. Yu. , Zabara P. P. , Bazaliy V. V.
Published: November 2022

Aim. Investigate the influence of treatment of maize plants with restrictive biological products on the formation of productivity of lines - parent components to optimize the elements of cultivation technology. Methods. 3-factor field experiment; measuring and weighing - to determine the structure of the crop; methods of mathematical statistics (analysis of variance, rectilinear and curvilinear correlation and regression). Results. Studies have shown that for the maximum manifestation of the sign "mass of 1000 grains" the optimal density is 70,000 plants ha-1. The increase in yield is positively influenced by the increase in the weight of 1000 seeds, which is due to both the genotype of the lines and the use of biologically active drugs Bio-gel, Helafit-combi. With the use of the drug Bio-gel laboratory germination increased by an average of 1.5 %, with the use of the drug Helafit-combi seed germination increased by 2.4 %. Conclusions. The maximum yield of the parent component DK 247 was observed at a density of 80 thousand plants/ha and treatment with the drug Helafit-combi - 4.89 t/ha. Mid-late lines - parent components DK 411 and DK 445 showed the highest yields at densities of 70,000 plants ha-1 and treatment with Helafit®-combi - 4.65 and 6.30 t/ha, respectively.
Hazel (Corylus L.) and hazelnut (Corylus domestica Kos. Et Opal.) pollen
Authors: Kosenko I. S. , Opalko A. I. , Balabak O. A. , Opalko O. A. , Oksantyuk V. M.
Published: November 2022

Aim. Wild hazel species (Corylus L.), and hazelnut (Corylus domestica Kos. et Opal.) cultivars are the monoecious anemophilous plants of the birch family (Betulaceae Gray). Due to the early spring catkins bloom of most Corylus, which occurs in March–April in Ukraine, their flowers may be damaged by late spring frosts, causing a decrease in the yield of nuts. That's why the study of the characteristics of the male gametophyte is of general scientific and applied importance for breeding and fruit growing. Methods. Study of pollen of hazelnut cultivars, representatives of C. avellana L., and C. chinensis Franch. from the collection of NDP "Sofiyivka" of the National Academy of Sciences of Ukraine was done using standard methods. Results. It turned out that the pollen of the ‘Sofiyivsky 15’ (cultivar selected from the hybrid population of ‘Garibaldi’×C. chinensis seedlings) had a greater likeness to paternal parent pollen than maternal parent pollen. According to the fertility, size, and shape of pollen grains, the pollen studied by Corylus spp. is characterized by a slight cultivar-form-species polymorphism. Conclusions. The studied species and forms of Corylus L., and Corylus domestica Kos. et Opal. cultivars were characterized by sufficient levels of pollen fertility for their cultivation in hazelnut orchards of all agro-climatic zones of Ukraine.
Assessment of wheat wide hybridization derivatives for agronomic traits and disease resistance in drought conditions
Authors: Motsnyi I. I. , Molodchenkova O. O. , Nargan T. P. , Nakonechnyi M. Yu. , Lyfenko S. Ph. , Fanin J. S. , Mishchenko L. T.
Published: November 2022

Aim. To determine the breeding value of wheat wide hybridization derivatives by studying the resistance to diseases, protein content and yield, productivity and drought resistance index. Methods. Growing plants in favorable conditions, environments of acute drought and under infection background of wheat rusts was combined with the laboratory methods for determining weight of 1000 kernels and protein content. Results. Disease resistance has depended on the pathogen species and the source of alien variability. The drought resistance index is mainly determined by the yield of the material in drought conditions (r = –0.65***) and to a lesser extent depends on the yield under normal environments (r = 0.27**). Conclusions. Resistance to leaf or yellow rust has been successfully transferred from all sources involved in hybridization, but to stem rust – mainly from the sample, containing the translocation 1BL.1RS in the karyotype and T. timopheevii in pedigree. When determining the drought resistance of the material, it is necessary to take into account not only the drought resistance index, but also its yield in favorable environments. The parameters of protein yield and absolute protein content in 1000 kernels should be used for the selection of promising breeding lines. The perspective lines with group disease resistance, high values of WTK, protein content, yield and drought resistance have been selected. Lines with leaf pubescence from T. timopheevii were characterized by low productivity.
Relationship between the stem color and the ear spines presense with economically valuable traits of bread winter wheat
Authors: Fedorova V. R. , Fait V. I. , Zelenina H. A.
Published: November 2022

Determination of the breeding value of the presence or absence of ear spines and anthocyanin stem color in bread winter wheat plants in the steppes of the Black Sea coast of Ukraine. Methods. Growing plants in the field. Phenological observations, weight of 1000 grains, analysis of variance and statistical significantly of the difference by t - criterion in pairwise comparison. Results. Groups of lines with the presence or absence of ear spines and with anthocyanin or green color, as well as with different combinations of both phenotypic traits did not differ significantly in economically valuable traits, with one exception. Plants with anthocyanin-colored stems were, on average, statistical significantly five centimeters taller than those with green color. Among the lines that exceeded the standard varieties Antonivka and Kuialnyk, in terms of yield, there are lines of all possible combinations of stem color and the presence or absence of spines. Conclusions. The phenotypic differences of recombinant lines in stem color (anthocyanin or green) or the presence or absence of spines, as well as their various combinations, are not significantly related to differences in genotypes before earing period, plant height, 1000 grain weight and grain yield.
Influence of meat flavored food additives on the occurrence of dominant lethal mutations in Drosophila melanogasfer
Authors: Kryzhanovska M. M. , Holub N. Ya. , Prokopiak M. Z. , Holinei H. M.
Published: November 2022

Aim. To investigate the mutagenic effect of meat flavored food additives of the Indasia food company on the occurrence of dominant lethal mutations in Drosophila melanogaster. Methods. The method of the accounting of dominant lethal mutations (DLM) in Drosophila melanogaster was used. This method allows recording early and late embryonic death. Results. Using the meat flavored food additive “Spices for grilled white sausages” in the recommended dose caused a decrease in the number of eggs by 45 %, according to the control group. An increase in the number of eggs with early embryonic death by 3 times, and eggs with late embryonic death by 5.5 times was noted (P < 0.95). A 5-fold increase in the frequency of DLMs compared to the control group was established. Using the meat flavored food additive “Flavoring with the taste of pork” in the recommended dose caused a decrease in the number of eggs by 13 %, according to the control group. Using this meat flavored food additive in a dose increased by 10 times caused a decrease in the number of eggs by 28 %, according to the control group. The recommended dose caused an increase the early embryonic death which was 3.6 times higher than in the control group (P > 0.95), and an increase late embryonic death which was 4.8 times higher than in the control group (P > 0.95). The frequency of DLMs in the recommended dose was 6.49 %, and in the tenfold increased dose – 8.67 %, according to the control group. Conclusions. The meat flavored food additives of the Indasia food company have a genotoxic effect in the recommended dose and cause the mutagenic activity and an increase of the frequency of occurrence of dominant lethal mutations in D. melanogaster.
Protective effect of zinc complex with hypoxanthine-9-riboside on wheat seedlings grown from gamma-irradiated seeds
Authors: Shamilov E. N. , Abdullayev A. S. , Shamilli V. E. , Azizov I. V.
Published: November 2022

Aim. The aim of the research was to obtain the zinc complex hypoxanthine-9-riboside and to study its effect during γ-irradiation on the biosynthesis of chlorophylls, carotenoids and on the release of chromosome aberrations in anaphase root hair cells in wheat seedlings. Methods. The zinc complex was obtained by direct interaction of zinc chloride – ZnCl2 with hypoxanthine-9-riboside. X-ray phase analysis and thermogravimetric measurements of the obtained complex were carried out. Before irradiation, seeds of durum wheat Triticum durum L. from a 60Co source were treated with a zinc complex with hypoxanthine-9-riboside at concentrations of 0.1; 0.01; 0.001%. Structural changes in chromosomes were determined in the initial and final stages of anaphase. Determination of chlorophylls and carotenoids was carried out according to Shlyk. Results. g-irradiation at doses of 50, 100 and 200 Gy has a significant effect on the content of green pigments and carotenoids in wheat seedlings. Under the action of g-irradiation, the content of chlorophyll decreases more than carotenoids. With an increase in the dose of radiation, a slight increase in the content of carotenoids is noted. In all variants, chromosomal abnormalities were found: the formation of fragments in metaphase and anaphase, bridges in anaphase, chromosome delays, uneven division of chromosomes. Conclusions. For the first time, it was found that the Zn (II) complex of hypoxanthine-9-riboside at the indicated concentrations significantly reduces the damaging effect of γ-irradiation, helps to eliminate abnormalities in mitotic division in wheat root hair cells.
The Colonial Experience of the British Empire of the Interbellum era and the RAND Corporation at the End of the XX Century: the Transfer of Ideas.
Authors: Nesterov Dmitrii Aleksandrovich
Published: November 2022

The author analyzes the features of the expert activity of the RAND Corporation at the end of the twentieth century, aimed at studying the colonial experience of counterinsurgency activities of the British Empire during the interwar period. The position of the American analytical center on the possibility of using such experience in modern conditions is considered. A comparison is made of the conclusions of RAND Corporation experts on a similar issue in an earlier historical period - the Cold War era. The reasons for the change in the views of the experts of the analytical center on the question of the potential possibility of applying the colonial experience of the counterinsurgency of the British Empire are revealed. Thus, the characteristic features of the academic examination of US foreign policy decisions at the end of the twentieth century are analyzed. The analysis showed that the RAND Corporation considered this experience useful to the United States, since this analytical center believed that the United States after the end of the Cold War found itself in the same position as the United Kingdom after the First World War. At the same time, the United States repeats the same mistakes that the British Empire authorities made – reducing spending on the army, shifting the emphasis from using infantry in anti-insurgency operations to modern weapons, spreading pacifist ideas and reducing the level of military training. Also, the RAND Corporation for the first time evaluated the colonial experience of the British Empire in a negative way, thereby advocating a return transfer. The United States of the late twentieth century, according to the analytical center, should make decisions not similar to those taken by the British authorities in the interwar period, but opposite to them. This approach is explained by the fact that the RAND Corporation had to provide information support for increasing spending on the US armed forces and maintaining large contingents of troops.
Design of a single glass tube optical lens for soft X-ray laser decoherence
Authors: Yuan Tian-Yu , Shao Shang-Kun , Sun Xue-Peng , Li Hui-Quan , Hua Lu , Sun Tian-Xi ,   Key Laboratory of Beam Technology of the Ministry of Education, College of Nuclear Science and Technology, Beijing Normal University, Beijing 100875, China,   Institute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, China
Published: November 2022

Laser has the advantages of high brightness, good monochromaticity, high coherence and good directionality, however, in some cases such as laser imaging and laser processing where only its high brightness or high monochromaticity is desired, the interference effect caused by high coherence can affect and limit its effective applications. In this work, a new single glass tube decoherence lens (SGTDL) is designed for soft X-ray laser decoherence through the simulation calculations. The simulation results show that an SGTDL with an entrance diameter of 5 mm, exit diameter of 0.6 mm and a length of 15 cm can effectively reduce the coherence of the X-ray laser with a wavelength of 10 nm and a beam waist radius of 1.25 mm. At the same time, the exit beam with a divergence range of 30–50 mrad is obtained at the SGTDL’s exit, and the transmission efficiency and gain in power density of the SGTDL are 78% and 52.74, respectively. For a laser beam with a wavelength of up to 1 nm, this model of SGTDL can maintain the transmission efficiency of the beam at more than 30%. This work also discusses the influence of the X-ray laser energy and the SGTDL’s length on the transmission performances of the SGTDL. The results show that the SGTDL designed according to the total reflection principle can meet the application requirements for laser decoherence in a range from the extreme ultraviolet to X-ray wavelength, and has a wide application prospect in X-ray laser imaging, laser processing, etc.
Open Science, the alliance way
Authors: Stoy Lennart , Kotar Mojca , Losada Marina , Casaldàliga Anna
Published: November 2022

Universities are challenged with creating an enabling environment for Open Science, including policies, technical infrastructure, services, training and support and cross-cutting issues such as FAIR research data management and Open Access. In many universities, staff and skills to supply are in high demand and the level of implementation is often lower than the strategic importance given to Open Science (EUA, 2021). Collaboration is crucial to address these challenges, be efficient and avoid duplication of efforts across higher education institutions and other stakeholders.
European university alliances are one mechanism that supports such collaborations (Council of the EU, 2021). In the EUTOPIA European University, an alliance consisting of 10 member universities from across Europe, an ambitious work programme has been developed and is implemented through several projects funded by Erasmus+ and Horizon 2020. EUTOPIA understands Open Science as a core pillar, recognizing that knowledge needs to be accessible for everyone. Therefore, our alliance aims to mainstream Open Science across the network and create closer policy alignment between individual alliance members.
We do so by addressing different dimensions relevant for Open Science, from policies over to joint training and support, as well as coordinating these activities across the alliance and policy alignment (implemented in a subsidiary way). Overall, our activities cover:

Building technical infrastructure, although challenging across institutional and national boundaries, through a collaboration with OpenAIRE. This will facilitate FAIRness of our research results and FAIR-compliance of institutional repositories.
Training and guidance on crucial issues such as research data management, Open Access, Citizen Science, and reproducible research for different target audiences.
A framework policy for research assessment & metrics considering Open Science as a cross-cutting issue.
Citizen science receives special attention through the establishment of a community of practice and regular citizen science clinics, informal meet-ups to share experience, approaches and challenges in Citizen Science.
A template Open Science policy to be implemented in modular fashion by partner universities and the creation of an alliance-wide Open Science office.

In short, European Universities are an important vehicle for connecting Open Science expertise and capacity across higher education institutions. They allow for the integration and efficient use of local expertise and networks for the benefit of the academic community of all members. Moreover, they are platforms for experimentation and sharing experience among partners with a focus on producing tangible results and improvements. We believe that alliances such as EUTOPIA are important tools to advance Open Science in Europe.