bluuebunny/crossref_metadata_2025 huggingface dataset:
Computation and Hypercomputation
Authors: Powell Andrew
Published: March 2022

This paper shows some of the differences and similarities between computation and hypercomputation, the similarities relating to the complexity of propositional computation and the differences being the propositions that can be decided computationally or hypercomputationally. The methods used are ordinal Turing machines with infinitely long programs and diagonalization out of computing complexity classes. The main results are the characterization of inequalities of run time complexities of serial, indeterministic serial and parallel computers and hypercomputers and the specification of a hierarchy of hypercomputers that can hypercompute the truths of all propositions in the standard class model of set theory, the von Neumann hierarchy of pure sets.
In Vivo Validation of a Cardiovascular Simulation Model in Pigs
Authors: Habigt Moriz A. , Gesenhues Jonas , Stemmler Maike , Hein Marc , Rossaint Rolf , Mechelinck Mare
Published: March 2022

Many computer simulation models of the cardiovascular system, of varying complexity and objectives, have been proposed in physiological science. Every model needs to be parameterized and evaluated individually. We conducted a porcine animal model to parameterize and evaluate a computer simulation model, recently proposed by our group. The results of an animal model, on thirteen healthy pigs, were used to generate consistent parameterization data for the full heart computer simulation model. To evaluate the simulation model, differences between the resulting simulation output and original animal data were analysed. The input parameters of the animal model, used to individualize the computer simulation, showed high interindividual variability (range of coefficient of variation: 10.1–84.5%), which was well-reflected by the resulting haemodynamic output parameters of the simulation (range of coefficient of variation: 12.6–45.7%). The overall bias between the animal and simulation model was low (mean: −3.24%, range: from −26.5 to 20.1%). The simulation model used in this study was able to adapt to the high physiological variability in the animal model. Possible reasons for the remaining differences between the animal and simulation model might be a static measurement error, unconsidered inaccuracies within the model, or unconsidered physiological interactions.
Normal Weight 6–12 Years Boys Demonstrate Better Cognitive Function and Aerobic Fitness Compared to Overweight Peers
Authors: Borkertienė Vaida , Valonytė-Burneikienė Laura
Published: March 2022

Background and Objectives: This study evaluated and compared the cognitive function (CF) and aerobic fitness (AF) of 15 normal-weight (NW) and 15 overweight (OW) children, aged 6–12 years. In addition, the relationship between CF and AF was evaluated. Materials and Methods: The ANAM4 battery was used to evaluate CF, and a constant treadmill walking exercise (6 km/h for 6 min) and a progressive treadmill exercise (modified Balke test) were used to assess pulmonary oxygen uptake (VO2). Results: The OW children displayed worse attention and visual tracking (88.95 ± 4.45% and 93.75 ± 3.16%), response inhibition (90.27 ± 1.54% and 93.67 ± 2%), and speed of processing (93.65 ± 1.5% and 94.4 ± 1.54%) than the NW children (p < 0.05). The VO2 max was higher and the time constant of VO2 kinetics was shorter in NW children (56.23 ± 3.53 mL/kg/min and 21.73 ± 1.57 s, respectively) than in OW children (45.84 ± 1.89 mL/kg/min and 33.46 ± 2.9 s, respectively; p < 0.05). Conclusion: The OW children aged 6–12 years demonstrated poorer CF and lower AF than their NW peers. An association between AF and CF indicators was identified in both groups.
The Role of Extracellular Vesicles in Osteoporosis: A Scoping Review
Authors: Zhang Weifei , Huang Pengzhou , Lin Jianjing , Zeng Hui
Published: March 2022

As an insidious metabolic bone disease, osteoporosis plagues the world, with high incidence rates. Patients with osteoporosis are prone to falls and becoming disabled, and their cone fractures and hip fractures are very serious, so the diagnosis and treatment of osteoporosis is very urgent. Extracellular vesicles (EVs) are particles secreted from cells to the outside of the cell and they are wrapped in a bilayer of phospholipids. According to the size of the particles, they can be divided into three categories, namely exosomes, microvesicles, and apoptotic bodies. The diameter of exosomes is 30–150 nm, the diameter of microvesicles is 100–1000 nm, and the diameter of apoptotic bodies is about 50–5000 nm. EVs play an important role in various biological process and diseases including osteoporosis. In this review, the role of EVs in osteoporosis is systematically reviewed and some insights for the prevention and treatment of osteoporosis are provided.
In Silico Drug Design of Benzothiadiazine Derivatives Interacting with Phospholipid Cell Membranes
Authors: Hu Zheyao , Marti Jordi
Published: March 2022

The use of drugs derived from benzothiadiazine, a bicyclic heterocyclic benzene derivative, has become a widespread treatment for diseases such as hypertension, low blood sugar or the human immunodeficiency virus, among others. In this work we have investigated the interactions of benzothiadiazine and four of its derivatives designed in silico with model zwitterionic cell membranes formed by dioleoylphosphatidylcholine, 1,2-dioleoyl-sn-glycero-3-phosphoserine and cholesterol at the liquid–crystal phase inside aqueous potassium chloride solution. We have elucidated the local structure of benzothiadiazine by means of microsecond molecular dynamics simulations of systems including a benzothiadiazine molecule or one of its derivatives. Such derivatives were obtained by the substitution of a single hydrogen site of benzothiadiazine by two different classes of chemical groups, one of them electron-donating groups (methyl and ethyl) and another one by electron-accepting groups (fluorine and trifluoromethyl). Our data have revealed that benzothiadiazine derivatives have a strong affinity to stay at the cell membrane interface although their solvation characteristics can vary significantly—they can be fully solvated by water in short periods of time or continuously attached to specific lipid sites during intervals of 10–70 ns. Furthermore, benzothiadiazines are able to bind lipids and cholesterol chains by means of single and double hydrogen-bonds of characteristic lengths between 1.6 and 2.1 Å.
Anion-Conducting Polymer Electrolyte without Ether Linkages and with Ionic Groups Grafted on Long Side Chains: Poly(Alkylene Biphenyl Butyltrimethyl Ammonium) (ABBA)
Authors: Narducci Riccardo , Becerra-Arciniegas Raul Andres , Pasquini Luca , Ercolani Gianfranco , Knauth Philippe , Di Vona Maria Luisa
Published: March 2022

In this work we report the synthesis of the new ionomer poly(alkylene biphenyl butyltrimethyl ammonium) (ABBA) with a backbone devoid of alkaline-labile C-O-C bonds and with quaternary ammonium groups grafted on long side chains. The ionomer was achieved by metalation reaction with n-butyllithium of 2-bromobiphenyl, followed by the introduction of the long chain with 1,4-dibromobutane. The reaction steps were followed by 1H-NMR spectroscopy showing the characteristic signals of the Br-butyl chain and indicating the complete functionalization of the biphenyl moiety. The precursor was polycondensed with 1,1,1-trifluoroacetone and then quaternized using trimethylamine (TMA). After the acid catalyzed polycondensation, the stoichiometric ratio between the precursors was respected. The quaternization with TMA gave a final degree of amination of 0.83 in agreement with the thermogravimetric analysis and with the ion exchange capacity of 2.5 meq/g determined by acid–base titration. The new ionomer blended with poly(vinylalcohol) (PVA) or poly(vinylidene difluoride) (PVDF) was also characterized by water uptake (WU) and ionic conductivity measurements. The higher water uptake and ionic conductivity observed with the PVDF blend might be related to a better nanophase separation.
Impact of Pre-Ozonation during Nanofiltration of MBR Effluent
Authors: Amadou-Yacouba Zoulkifli , Mendret Julie , Lesage Geoffroy , Zaviska François , Brosillon Stephan
Published: March 2022

This study aimed to investigate the impact of real MBR effluent pre-ozonation on nanofiltration performances. Nanofiltration experiments were separately run with non-ozonated real MBR effluent, ozonated real MBR effluent and synthetic ionic solution mimicking the ionic composition of the real MBR effluent. The specific UV absorbance and the chemical oxygen demand were monitored during ozonation of real effluent, and the mineralization rate was calculated through the quantitative analysis of dissolved organic carbon. The membrane structure was characterized using SEM on virgin and fouled membrane surfaces and after different cleaning steps. The results confirm the low effect of the ozonation process in terms of organic carbon mineralization. However, the chemical oxygen demand and the specific UV absorbance were decreased by 50% after ozonation, demonstrating the efficiency of ozonation in degrading a specific part of the organic matter fraction. A benefic effect of pre-ozonation was observed, as it limits both fouling and flux decrease. This study shows that the partial mineralization of dissolved and colloidal organic matter by ozonation could have a positive effect on inorganic scaling and decrease severe NF membrane fouling.
Proton Conductivity Enhancement at High Temperature on Polybenzimidazole Membrane Electrolyte with Acid-Functionalized Graphene Oxide Fillers
Authors: Sulaiman Raja Rafidah Raja , Walvekar Rashmi , Wong Wai Yin , Khalid Mohammad , Pang Ming Meng
Published: March 2022

Graphene oxide (GO) and its acid-functionalized form are known to be effective in enhancing the proton transport properties of phosphoric-acid doped polybenzimidazole (PA-doped PBI) membranes utilized in high-temperature proton exchange membrane fuel cells (HTPEMFC) owing to the presence of proton-conducting functional groups. This work aims to provide a comparison between the different effects of GO with the sulfonated GO (SGO) and phosphonated GO (PGO) on the properties of PA-doped PBI, with emphasis given on proton conductivity to understand which functional groups are suitable for proton transfer under high temperature and anhydrous conditions. Each filler was synthesized following existing methods and introduced into PBI at loadings of 0.25, 0.5, and 1 wt.%. Characterizations were carried out on the overall thermal stability, acid doping level (ADL), dimensional swelling, and proton conductivity. SGO and PGO-containing PBI exhibit better conductivity than those with GO at 180 °C under anhydrous conditions, despite a slight reduction in ADL. PBI with 0.5 wt.% SGO exhibits the highest conductivity at 23.8 mS/cm, followed by PBI with 0.5 wt.% PGO at 19.6 mS/cm. However, the membrane with PGO required a smaller activation energy for proton conduction, thus less energy was needed to initiate fast proton transfer. Additionally, the PGO-containing membrane also displayed an advantage in its thermal stability aspect. Therefore, considering these properties, it is shown that PGO is a potential filler for improving PBI properties for HTPEMFC applications.
A Review of Titanium Dioxide (TiO2)-Based Photocatalyst for Oilfield-Produced Water Treatment
Authors: Dharma Hadi Nugraha Cipta , Jaafar Juhana , Widiastuti Nurul , Matsuyama Hideto , Rajabsadeh Saied , Othman Mohd Hafiz Dzarfan , Rahman Mukhlis A , Jafri Nurul Natasha Mohammad , Suhaimin Nuor Sariyan , Nasir Atikah Mohd , Alias Nur Hashimah
Published: March 2022

Oilfield produced water (OPW) has become a primary environmental concern due to the high concentration of dissolved organic pollutants that lead to bioaccumulation with high toxicity, resistance to biodegradation, carcinogenicity, and the inhibition of reproduction, endocrine, and non-endocrine systems in aquatic biota. Photodegradation using photocatalysts has been considered as a promising technology to sustainably resolve OPW pollutants due to its benefits, including not requiring additional chemicals and producing a harmless compound as the result of pollutant photodegradation. Currently, titanium dioxide (TiO2) has gained great attention as a promising photocatalyst due to its beneficial properties among the other photocatalysts, such as excellent optical and electronic properties, high chemical stability, low cost, non-toxicity, and eco-friendliness. However, the photoactivity of TiO2 is still inhibited because it has a wide band gap and a low quantum field. Hence, the modification approaches for TiO2 can improve its properties in terms of the photocatalytic ability, which would likely boost the charge carrier transfer, prevent the recombination of electrons and holes, and enhance the visible light response. In this review, we provide an overview of several routes for modifying TiO2. The as-improved photocatalytic performance of the modified TiO2 with regard to OPW treatment is reviewed. The stability of modified TiO2 was also studied. The future perspective and challenges in developing the modification of TiO2-based photocatalysts are explained.
Microstructure and Fracture Performance of Wire Arc Additively Manufactured Inconel 625 Alloy by Hot-Wire GTAW
Authors: Wang Xiaoli , Hu Qingxian , Li Tianqing , Liu Wenkang , Tang Douxi , Hu Zichen , Liu Kang
Published: March 2022

In this work, an Inconel 625 thin-wall structure was fabricated by the gas tungsten arc welding (GTAW) hot-wire arc additive manufacturing process. The microstructure and mechanical properties of the Inconel 625 samples, extracted from different orientations and locations of the thin-wall structure, were investigated and compared. The results showed that the additively manufactured Inconel 625 component, made by hot-wire GTAW, had good quality. Its microstructure consisted of dendrites, equiaxial crystals, and cellular crystals. The average hardness from the bottom to the top was similar, indicating that the thin wall had good consistency. The plasticity in the deposition direction was better than those in the other three regions, which was related to the dendritic structure in the sedimentary direction.
Experimental and Modeling Study of Deformability of Glassy CaO-(MnO)-Al2O3-SiO2 Inclusions
Authors: Shu Qifeng , You Chaoge , Alatarvas Tuomas , Fabritius Timo Matti Juhani
Published: March 2022

The occurrence of non-deformable, non-metallic inclusions is the dominant reason for failure of wire during drawing and degrades service life for some steel grades, e.g., tire cord steel. To investigate the deformability of glassy inclusions in CaO-Al2O3-SiO2 and MnO-Al2O3-SiO2 systems, experimental and numerical methods were used. Young’s modulus values of some glasses based on the CaO-Al2O3-SiO2 and MnO-Al2O3-SiO2 systems, which correspond to typical inclusions in tire cord steel, were measured with resonant ultrasound spectroscopy. The effect of basicity, defined as the ratio of mass percentage of CaO to SiO2, on Young’s modulus and Poisson’s ratio were investigated. The Young’s moduli of glasses are enhanced with increasing basicity, which could be attributed to the high field strength of calcium ions. The Poisson’s ratios of glasses also show an increase tendency with increasing basicity, which could be due to the loss of rigidity of network with introduction of calcium ions. The equations in the literature for Young’s modulus calculation were evaluated based on the present and literature data. Appen’s equation is modified by re-fitting the present and literature data to give accurate estimation of Young’s modulus with the mean deviation of 2%. The iso-Young’s modulus diagrams for CaO-Al2O3-SiO2 systems were constructed. It is proposed that the iso-Young’s modulus diagram could be combined with liquid area in CaO-Al2O3-SiO2 ternary phase diagram to optimize the inclusion composition during both hot rolling and cold drawing.
INDY as a Therapeutic Target for Cardio-Metabolic Disease
Authors: Pesta Dominik , Jordan Jens
Published: March 2022

Decreased expression of the plasma membrane citrate transporter INDY (acronym I’m Not Dead, Yet) promotes longevity and protects from high-fat diet- and aging-induced metabolic derangements. Preventing citrate import into hepatocytes by different strategies can reduce hepatic triglyceride accumulation and improve hepatic insulin sensitivity, even in the absence of effects on body composition. These beneficial effects likely derive from decreased hepatic de novo fatty acid biosynthesis as a result of reduced cytoplasmic citrate levels. While in vivo and in vitro studies show that inhibition of INDY prevents intracellular lipid accumulation, body weight is not affected by organ-specific INDY inhibition. Besides these beneficial metabolic effects, INDY inhibition may also improve blood pressure control through sympathetic nervous system inhibition, partly via reduced peripheral catecholamine synthesis. These effects make INDY a promising candidate with bidirectional benefits for improving both metabolic disease and blood pressure control.
Pudding Proteomics: Cyclomaltodextrin Glucanotransferase and Microbial Proteases Can Liquefy Extended Shelf Life Dairy Products
Authors: Kleinwort Kristina J. H. , Weigand Maria , Hoffmann Lydia , Degroote Roxane L. , Dietrich Richard , Märtlbauer Erwin , Hauck Stefanie M. , Deeg Cornelia A.
Published: March 2022

In recent years, a lack of stability of dairy products with extended shelf life (e.g., yoghurt products, UHT desserts) has occurred, with the corresponding products liquefying significantly after days or weeks. This project aimed to identify the enzymes responsible for the liquefaction of the affected products based on differential proteomic analyses. No evidence was found for the presence of starch-degrading bacteria in the affected products. With zymography and proteome analysis, we detected the cause of liquefaction in a pudding by contamination of its aroma component with an engineered amylolytic enzyme, cyclomaltodextrin glucanotransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes. In addition, we detected contamination with Pseudomonas-derived proteolytic ATP-dependent Clp protease in one pudding batch and proteases in technically used amylases, which degraded β-caseins in another batch. Identification of these agents with liquefying properties in dairy products are useful for adjustment of production protocols and/or composition of additives, and thus shelf life extension.
Insight into the Evolving Role of PCSK9
Authors: Maligłówka Mateusz , Kosowski Michał , Hachuła Marcin , Cyrnek Marcin , Bułdak Łukasz , Basiak Marcin , Bołdys Aleksandra , Machnik Grzegorz , Bułdak Rafał Jakub , Okopień Bogusław
Published: March 2022

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is the last discovered member of the family of proprotein convertases (PCs), mainly synthetized in hepatic cells. This serine protease plays a pivotal role in the reduction of the number of low-density lipoprotein receptors (LDLRs) on the surface of hepatocytes, which leads to an increase in the level of cholesterol in the blood. This mechanism and the fact that gain of function (GOF) mutations in PCSK9 are responsible for causing familial hypercholesterolemia whereas loss-of-function (LOF) mutations are associated with hypocholesterolemia, prompted the invention of drugs that block PCSK9 action. The high efficiency of PCSK9 inhibitors (e.g., alirocumab, evolocumab) in decreasing cardiovascular risk, pleiotropic effects of other lipid-lowering drugs (e.g., statins) and the multifunctional character of other proprotein convertases, were the cause for proceeding studies on functions of PCSK9 beyond cholesterol metabolism. In this article, we summarize the current knowledge on the roles that PCSK9 plays in different tissues and perspectives for its clinical use.
Metabolic Footprinting of Microbial Systems Based on Comprehensive In Silico Predictions of MS/MS Relevant Data
Authors: Reiter Alexander , Asgari Jian , Wiechert Wolfgang , Oldiges Marco
Published: March 2022

Metabolic footprinting represents a holistic approach to gathering large-scale metabolomic information of a given biological system and is, therefore, a driving force for systems biology and bioprocess development. The ongoing development of automated cultivation platforms increases the need for a comprehensive and rapid profiling tool to cope with the cultivation throughput. In this study, we implemented a workflow to provide and select relevant metabolite information from a genome-scale model to automatically build an organism-specific comprehensive metabolome analysis method. Based on in-house literature and predicted metabolite information, the deduced metabolite set was distributed in stackable methods for a chromatography-free dilute and shoot flow-injection analysis multiple-reaction monitoring profiling approach. The workflow was used to create a method specific for Saccharomyces cerevisiae, covering 252 metabolites with 7 min/sample. The method was validated with a commercially available yeast metabolome standard, identifying up to 74.2% of the listed metabolites. As a first case study, three commercially available yeast extracts were screened with 118 metabolites passing quality control thresholds for statistical analysis, allowing to identify discriminating metabolites. The presented methodology provides metabolite screening in a time-optimised way by scaling analysis time to metabolite coverage and is open to other microbial systems simply starting from genome-scale model information.
Association between Visceral Adipose Tissue Metabolism and Alzheimer’s Disease Pathology
Authors: Kim Shin , Yi Hyon-Ah , Won Kyoung Sook , Lee Ji Soo , Kim Hae Won
Published: March 2022

The visceral adipose tissue (VAT) has been recognized as an endocrine organ, and VAT dysfunction could be a risk factor for Alzheimer’s disease (AD). We aimed to evaluate the association of VAT metabolism with AD pathology. This cross-sectional study included 54 older subjects with cognitive impairment who underwent 2-deoxy-2-[fluorine-18]-fluoro-D-glucose (18F-FDG) torso positron emission tomography (PET) and 18F-florbetaben brain PET. 18F-FDG uptake in VAT on 18F-FDG PET images was used as a marker of VAT metabolism, and subjects were classified into high and low VAT metabolism groups. A voxel-based analysis revealed that the high VAT metabolism group exhibited a significantly higher cerebral amyloid-β (Aβ) burden than the low VAT metabolism group. In the volume-of-interest analysis, multiple linear regression analyses with adjustment for age, sex, and white matter hyperintensity volume revealed that 18F-FDG uptake in VAT was significantly associated with the cerebral Aβ burden (β = 0.359, p = 0.007). In conclusion, VAT metabolism was associated with AD pathology in older subjects. Our findings suggest that VAT dysfunction could contribute to AD development.
Whole Exome/Genome Sequencing Joint Analysis of a Family with Oligogenic Familial Hypercholesterolemia
Authors: Ghaleb Youmna , Elbitar Sandy , Philippi Anne , El Khoury Petra , Azar Yara , Andrianirina Miangaly , Loste Alexia , Abou-Khalil Yara , Nicolas Gaël , Le Borgne Marie , Moulin Philippe , Di-Filippo Mathilde , Charrière Sybil , Farnier Michel , Yelnick Cécile , Carreau Valérie , Ferrières Jean , Lecerf Jean-Michel , Derksen Alexa , Bernard Geneviève , Gauthier Marie-Soleil , Coulombe Benoit , Lütjohann Dieter , Fin Bertrand , Boland Anne , Olaso Robert , Deleuze Jean-François , Rabès Jean-Pierre , Boileau Catherine , Abifadel Marianne , Varret Mathilde
Published: March 2022

Autosomal Dominant Hypercholesterolemia (ADH) is a genetic disorder caused by pathogenic variants in LDLR, APOB, PCSK9 and APOE genes. We sought to identify new candidate genes responsible for the ADH phenotype in patients without pathogenic variants in the known ADH-causing genes by focusing on a French family with affected and non-affected members who presented a high ADH polygenic risk score (wPRS). Linkage analysis, whole exome and whole genome sequencing resulted in the identification of variants p.(Pro398Ala) in CYP7A1, p.(Val1382Phe) in LRP6 and p.(Ser202His) in LDLRAP1. A total of 6 other variants were identified in 6 of 160 unrelated ADH probands: p.(Ala13Val) and p.(Aps347Asn) in CYP7A1; p.(Tyr972Cys), p.(Thr1479Ile) and p.(Ser1612Phe) in LRP6; and p.(Ser202LeufsTer19) in LDLRAP1. All six probands presented a moderate wPRS. Serum analyses of carriers of the p.(Pro398Ala) variant in CYP7A1 showed no differences in the synthesis of bile acids compared to the serums of non-carriers. Functional studies of the four LRP6 mutants in HEK293T cells resulted in contradictory results excluding a major effect of each variant alone. Within the family, none of the heterozygous for only the LDLRAP1 p.(Ser202His) variant presented ADH. Altogether, each variant individually does not result in elevated LDL-C; however, the oligogenic combination of two or three variants reveals the ADH phenotype.
Monitoring Intracellular Metabolite Dynamics in Saccharomyces cerevisiae during Industrially Relevant Famine Stimuli
Authors: Minden Steven , Aniolek Maria , Sarkizi Shams Hajian Christopher , Teleki Attila , Zerrer Tobias , Delvigne Frank , van Gulik Walter , Deshmukh Amit , Noorman Henk , Takors Ralf
Published: March 2022

Carbon limitation is a common feeding strategy in bioprocesses to enable an efficient microbiological conversion of a substrate to a product. However, industrial settings inherently promote mixing insufficiencies, creating zones of famine conditions. Cells frequently traveling through such regions repeatedly experience substrate shortages and respond individually but often with a deteriorated production performance. A priori knowledge of the expected strain performance would enable targeted strain, process, and bioreactor engineering for minimizing performance loss. Today, computational fluid dynamics (CFD) coupled to data-driven kinetic models are a promising route for the in silico investigation of the impact of the dynamic environment in the large-scale bioreactor on microbial performance. However, profound wet-lab datasets are needed to cover relevant perturbations on realistic time scales. As a pioneering study, we quantified intracellular metabolome dynamics of Saccharomyces cerevisiae following an industrially relevant famine perturbation. Stimulus-response experiments were operated as chemostats with an intermittent feed and high-frequency sampling. Our results reveal that even mild glucose gradients in the range of 100 μmol·L−1 impose significant perturbations in adapted and non-adapted yeast cells, altering energy and redox homeostasis. Apparently, yeast sacrifices catabolic reduction charges for the sake of anabolic persistence under acute carbon starvation conditions. After repeated exposure to famine conditions, adapted cells show 2.7% increased maintenance demands.
A Preliminary Metabolomic Study of Yorkshire Terrier Enteropathy
Authors: Galler Alexandra I. , Klavins Kristaps , Burgener Iwan A.
Published: March 2022

Perturbations of metabolite profiles in human and canine enteropathies have been reported before. However, data in dogs are scarce and inconsistent. Currently, the metabolite profile in Yorkshire Terrier enteropathy (YTE) and the impact of treatment is unknown. The objective of this study was to investigate the plasma metabolome of 13 Yorkshire Terriers with YTE and compare it to 20 healthy Yorkshire Terriers. Furthermore, we studied the impact of treatment on the metabolome. In this prospective observational study, plasma metabolite profiles were analyzed by flow injection analysis-tandem mass spectrometry (FIA-MS/MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a targeted metabolomics kit. Metabolite analysis revealed that YTE is accompanied by changes in lipid and bile acid metabolism. YTE was associated with a significant decrease of long-chain fatty acids (octadecenoic acid, eicosadienoic acid, eicosatrienoic acid) and lower levels of long-chain acylcarnitines (tetradecanoylcarnitine, hexadecanoylcarnitine, hexadecenoylcarnitine, octadecenoylcarnitine) compared with healthy controls. Furthermore, taurodeoxycholic acid, a secondary bile acid, was decreased in plasma from YTE patients. These changes might be breed-specific and might be involved in the pathogenesis of YTE. Interestingly, changes in metabolite levels were not recovered after treatment and differed considerably from healthy controls.
Fractional Bandwidth up to 24% and Spurious Free SAW Filters on Bulk 15°YX-LiNbO3 Substrates Using Thickness-Modulated IDT Structures
Authors: Lu Zengtian , Fu Sulei , Xu Zhibin , Wang Weibiao , Zhang Qiaozhen , Zhang Jianrun , Zhang Hui
Published: March 2022

To cope with ubiquitous wireless connectivity and the increased and faster data delivery in 5G communication, surface acoustic wave (SAW) filters are progressively requiring wider bandwidths. Conventional bulk 15°YX-LiNbO3 substrates with a large coupling coefficient (K2) are attractive for the low-cost mass production of wideband SAW filters, but these generally suffer from spurious responses, limiting their practical application. In this work, a novel and simple SAW configuration is proposed that uses thickness-modulated interdigital transducer (IDT) structures to overcome the limitations set by spurious responses. Different from the conventional design where the thicknesses of the IDT electrodes in the series and parallel resonators generally kept the same, the proposed configuration adopts IDT electrodes of different thicknesses in the series and shunt resonators to suppress or remove unwanted spurious Rayleigh modes from the filter passband. Two different ultra-wideband SAW filter designs employing thickness-modulated IDTs were designed and fabricated to validate the effective suppression of spurious modes. The SAW filters experimentally featured spurious-free responses in the passband as well as a large 3 dB fractional bandwidth (FBW) in the 18.0% and 24.1% ranges and low insertion losses below 1 dB. This work can significantly broaden the range of applications for SAW devices and can open a pathway to commercialize ultra-wideband SAW filters in 5G communication systems.
Fine Texture Detection Based on a Solid–Liquid Composite Flexible Tactile Sensor Array
Authors: Liu Weiting , Zhang Guoshi , Zhan Binpeng , Hu Liang , Liu Tao
Published: March 2022

Surface texture information plays an important role in the cognition and manipulation of an object. Vision and touch are the two main methods for extracting an object’s surface texture information. However, vision is often limited since the viewing angle is uncertain during manipulation. In this article, we propose a fine surface texture detection method based on a stochastic resonance algorithm through a novel solid–liquid composite flexible tactile sensor array. A thin flexible layer and solid–liquid composite conduction structure on the sensor effectively reduce the attenuation of the contact force and enhance the sensitivity of the sensor. A series of ridge texture samples with different heights (0.9, 4, 10 μm), different widths (0.3, 0.5, 0.7, 1 mm), but the same spatial period (2 mm) of ridges were used in the experiment. The experimental results prove that the stochastic resonance algorithm can significantly improve the signal characteristic of the output signal of the sensor. The sensor has the capability to detect fine ridge texture information. The mean relative error of the estimation for the spatial period was 1.085%, and the ridge width and ridge height, respectively, have a monotonic mapping relationship with the corresponding model output parameters. The sensing capability to sense a fine texture of tactile senor surpasses the limit of human fingers.
A Simple Method for Fabricating Ink Chamber of Inkjet Printheads
Authors: Huang Zheguan , Tang Yang , Liu Zhibin , Zhang Xiaofei , Zhou Yan , Xie Yonglin
Published: March 2022

The process of fabricating chambers is becoming more important for inkjet printheads. However, there are some problems with the majority of present fabrication methods, such as nozzle structural deformation, blocked chambers, and collapsed chambers. In this paper, we propose a new process for preparing printhead chips by bonding tantalum nitride thin-film heaters and SU-8 chamber film using UV curing optical adhesive. This process simplifies the preparation process of printhead chips and overcomes the limitations of the traditional adhesive bonding process. Firstly, a chamber film was prepared by the molding lithography process based on a PDMS mold. The chamber film was then bonded with the membrane heater by the adhesive bonding process based on film transfer to form a thermal bubble printhead chip. Finally, the chip was integrated with other components to form a thermal inkjet printhead. The results show that the overflow width of bonding interface of 3.10 μm and bonding strength of 3.3 MPa were achieved. In addition, the printhead could stably eject polyvinyl pyrrolidone binder droplets, which are expected to be used for binder-jetting printing of powder such as ceramics, metals, and sand molds. These results might provide new clues to better understand the adhesive bonding process based on film transfer and the new applications of inkjet printheads.
Adaptive Sliding Mode Disturbance Observer and Deep Reinforcement Learning Based Motion Control for Micropositioners
Authors: Liang Shiyun , Xi Ruidong , Xiao Xiao , Yang Zhixin
Published: March 2022

The motion control of high-precision electromechanitcal systems, such as micropositioners, is challenging in terms of the inherent high nonlinearity, the sensitivity to external interference, and the complexity of accurate identification of the model parameters. To cope with these problems, this work investigates a disturbance observer-based deep reinforcement learning control strategy to realize high robustness and precise tracking performance. Reinforcement learning has shown great potential as optimal control scheme, however, its application in micropositioning systems is still rare. Therefore, embedded with the integral differential compensator (ID), deep deterministic policy gradient (DDPG) is utilized in this work with the ability to not only decrease the state error but also improve the transient response speed. In addition, an adaptive sliding mode disturbance observer (ASMDO) is proposed to further eliminate the collective effect caused by the lumped disturbances. The micropositioner controlled by the proposed algorithm can track the target path precisely with less than 1 μm error in simulations and actual experiments, which shows the sterling performance and the accuracy improvement of the controller.
Screen-Printing of Functionalized MWCNT-PEDOT:PSS Based Solutions on Bendable Substrate for Ammonia Gas Sensing
Authors: Boonthum Direk , Oopathump Chutima , Fuengfung Supasil , Phunudom Patipak , Thaibunnak Ananya , Juntong Nachapan , Rungruang Suvanna , Pakdee Udomdej
Published: March 2022

Multi-walled carbon nanotubes (MWCNTs) were grown on a stainless-steel foil by thermal chemical vapor deposition (CVD) process. The MWCNTs were functionalized with carboxylic groups (COOH) on their surfaces by using oxidation and acid (3:1 H2SO4/HNO3) treatments for improving the solubility property of them in the solvent. The functionalized MWCNTs (f-MWCNTs) were conducted to prepare the solution by continuous stir in poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS), dimethyl sulfoxide (DMSO), ethylene glycol (EG) and Triton X-100. The solution was deposited onto a bendable substrate such as polyethylene terephthalate (PET) with a fabricated silver interdigitated electrode for application in a room-temperature gas sensor. A homemade-doctor blade coater, an UNO R3 Arduino board and a L298N motor driver are presented as a suitable system for screen printing the solution onto the gas-sensing substrates. The different contents of f-MWCNTs embedded in PEDOT:PSS were compared in the gas response to ammonia (NH3), ethanol (C2H5OH), benzene (C6H6), and acetone (C3H6O) vapors. The results demonstrate that the 3.0% v/v of f-MWCNT solution dissolved in 87.8% v/v of PEDOT:PSS, 5.4% v/v of DMSO, 3.6% v/v of EG and 0.2% v/v of Triton X-100 shows the highest response to 80 ppm NH3. Finally, the reduction in the NH3 response under heavy substrate-bending is also discussed.
A 2.5 V, 2.56 ppm/°C Curvature-Compensated Bandgap Reference for High-Precision Monitoring Applications
Authors: Zhu Guangqian , Fu Zhaoshu , Liu Tingting , Zhang Qidong , Yang Yintang
Published: March 2022

This work presents a high-precision high-order curvature-compensated bandgap voltage reference (BGR) for battery monitoring applications. The collector currents of bipolar junction transistor (BJT) pairs with different ratios and temperature characteristics can cause greater nonlinearities in ΔVEB. The proposed circuit additionally introduces high-order curvature compensation in the generation of ΔVEB, such that it presents high-order temperature effects complementary to VEB. Fabricated using a 0.18 µm BCD process, the proposed BGR generates a 2.5 V reference voltage with a minimum temperature coefficient of 2.65 ppm/°C in the range of −40 to 125 °C. The minimum line sensitivity is 0.023%/V when supply voltage varies from 4.5 to 5.5 V. The BGR circuit area is 382 × 270 μm2, and the BMIC area is 2.8 × 2.8 mm2.
Investigation on the Thermal Characteristics of Enhancement-Mode p-GaN HEMT Device on Si Substrate Using Thermoreflectance Microscopy
Authors: Wang Hongyue , Yuan Chao , Xin Yajie , Shi Yijun , Zhong Yaozong , Huang Yun , Lu Guoguang
Published: March 2022

In this paper, thermoreflectance microscopy was used to measure the high spatial resolution temperature distribution of the p-GaN HEMT under high power density. The maximum temperature along the GaN channel was located at the drain-side gate edge region. It was found that the thermal resistance (Rth) of the p-GaN HEMT device increased with the increase of channel temperature. The Rth dependence on the temperature was well approximated by a function of Rth~Ta (a = 0.2). The three phonon Umklapp scattering, point mass defects and dislocations scattering mechanisms are suggested contributors to the heat transfer process for the p-GaN HEMT. The impact of bias conditions and gate length on the thermal characteristics of the device was investigated. The behaviour of temperature increasing in the time domain with 50 µs pulse width and different drain bias voltage was analysed. Finally, a field plate structure was demonstrated for improving the device thermal performance.
Electropolishing and Shaping of Micro-Scale Metallic Features
Authors: Zaki Sana , Zhang Nan , Gilchrist Michael D.
Published: March 2022

Electropolishing (EP) is most widely used as a metal finishing process. It is a non-contact electrochemical process that can clean, passivate, deburr, brighten, and improve the biocompatibility of surfaces. However, there is clear potential for it to be used to shape and form the topology of micro-scale surface features, such as those found on the micro-applications of additively manufactured (AM) parts, transmission electron microscopy (TEM) samples, micro-electromechanical systems (MEMs), biomedical stents, and artificial implants. This review focuses on the fundamental principles of electrochemical polishing, the associated process parameters (voltage, current density, electrolytes, electrode gap, and time), and the increasing demand for using environmentally sustainable electrolytes and micro-scale applications. A summary of other micro-fabrication processes, including micro-milling, micro-electric discharge machining (EDM), laser polishing/ablation, lithography (LIGA), electrochemical etching (MacEtch), and reactive ion etching (RIE), are discussed and compared with EP. However, those processes have tool size, stress, wear, and structural integrity limitations for micro-structures. Hence, electropolishing offers two-fold benefits of material removal from the metal, resulting in a smooth and bright surface, along with the ability to shape/form micro-scale features, which makes the process particularly attractive for precision engineering applications.zx3.
Design and Evaluation of a Button Sensor Antenna for On-Body Monitoring Activity in Healthcare Applications
Authors: Ali Shahid Muhammad , Sovuthy Cheab , Noghanian Sima , Saeidi Tale , Majeed Muhammad Faran , Hussain Amir , Masood Faisal , Khan Shariq Mahmood , Shah Syed Aziz , Abbasi Qammer H.
Published: March 2022

A button sensor antenna for on-body monitoring in wireless body area network (WBAN) systems is presented. Due to the close coupling between the sensor antenna and the human body, it is highly challenging to design sensor antenna devices. In this paper, a mechanically robust system is proposed that integrates a dual-band button antenna with a wireless sensor module designed on a printed circuit board (PCB). The system features a small footprint and has good radiation characteristics and efficiency. This was fabricated, and the measured and simulated results are in good agreement. The design offers a wide range of omnidirectional radiation patterns in free space, with a reflection coefficient (S11) of −29.30 (−30.97) dB, a maximum gain of 1.75 (5.65) dBi, and radiation efficiency of 71.91 (92.51)% in the lower and upper bands, respectively. S11 reaches −23.07 (−27.07) dB and −30.76 (−31.12) dB, respectively, with a gain of 2.09 (6.70) dBi and 2.16 (5.67) dBi, and radiation efficiency of 65.12 (81.63)% and 75.00 (85.00)%, when located on the body for the lower and upper bands, respectively. The performance is minimally affected by bending, movement, and fabrication tolerances. The specific absorption rate (SAR) values are below the regulatory limitations for the spatial average over 1 g (1.6 W/Kg) and 10 g of tissues (2.0 W/Kg). For both indoor and outdoor conditions, experimental results of the range tests confirm the coverage of up to 40 m.
Characterization of Active Electrode Yield for Intracortical Arrays: Awake versus Anesthesia
Authors: Sturgill Brandon , Radhakrishna Rahul , Thai Teresa , Patnaik Sourav , Capadona Jeffrey , Pancrazio Joseph
Published: March 2022

Intracortical microelectrode arrays are used for recording neural signals at single-unit resolution and are promising tools for studying brain function and developing neuroprosthetics. Research is being done to increase the chronic performance and reliability of these probes, which tend to decrease or fail within several months of implantation. Although recording paradigms vary, studies focused on assessing the reliability and performance of these devices often perform recordings under anesthesia. However, anesthetics—such as isoflurane—are known to alter neural activity and electrophysiologic function. Therefore, we compared the neural recording performance under anesthesia (2% isoflurane) followed by awake conditions for probes implanted in the motor cortex of both male and female Sprague-Dawley rats. While the single-unit spike rate was significantly higher by almost 600% under awake compared to anesthetized conditions, we found no difference in the active electrode yield between the two conditions two weeks after surgery. Additionally, the signal-to-noise ratio was greater under anesthesia due to the noise levels being nearly 50% greater in awake recordings, even though there was a 14% increase in the peak-to-peak voltage of distinguished single units when awake. We observe that these findings are similar for chronic time points as well. Our observations indicate that either anesthetized or awake recordings are acceptable for studies assessing the chronic reliability and performance of intracortical microelectrode arrays.
Precise Droplet Dispensing in Digital Microfluidics with Dumbbell-Shaped Electrodes
Authors: Wang Wei
Published: March 2022

Electro-wetting-on-dielectric (EWOD) enables the manipulation of droplets on a two-dimensional surface, which provides a versatile technique for digital microfluidics at a micro- or nano-scale. However, the deficiency of the dispensing precision has long limited its applications in micro total analysis systems (μ-TAS) where the accuracy of assays is largely determined by the volume control of the reagent dosing. This paper proposes optimum electrode designs and carries out characterization experiments to demonstrate the reproducibility of on-chip droplet generation with no extra external apparatus. The coefficient variation of the volumes of consecutively dispensed droplets from a non-refilling reservoir can be limited to below 0.3%, indicating the validity of the new electrode structure in practical applications.
Microparticle Transport and Sedimentation in a Rhythmically Expanding Alveolar Chip
Authors: Zhang Wei , Dong Jun , Lv Huimin , Bai Weitao , Lu Hongzhou , Noack Bernd R. , Zhu Yonggang , Yang Yue
Published: March 2022

Understanding the mechanism of particle transport and sedimentation in pulmonary alveolus is important for deciphering the causes of respiratory diseases and helping the development of drug delivery. In this study, taking advantage of the microfluidic technique, an experimental platform was developed to study particle behavior in a rhythmically expanding alveolar chip for a sufficient number of cycles. The alveolar flow patterns at different generations were measured for two cases with the gravity direction parallel or vertical to the alveolar duct. Affected by both the vortex flow inside the alveoli and the shear flow in the duct simultaneously, it was observed that particles inside the alveoli either escaped from the inlet of the alveolar duct or stayed in the alveoli, revealing the irreversibility of particle transport in the alveoli. At the earlier acinar generations, particles were inclined to deposit on the distal alveolar wall. The settling rates of particles of different sizes in the alveoli were also compared. This study provides valuable data for understanding particle transport and sedimentation in the alveoli.
Revising the Freshwater Thelohania to Astathelohania gen. et comb. nov., and Description of Two New Species
Authors: Stratton Cheyenne E. , Reisinger Lindsey S. , Behringer Donald C. , Bojko Jamie
Published: March 2022

Crayfish are common hosts of microsporidian parasites, prominently from the genus Thelohania. Thelohania is a polyphyletic genus, with multiple genetically distinct lineages found from freshwater and marine environments. Researchers have been calling for a revision of this group for over a decade. We provide evidence that crayfish-infecting freshwater Thelohania are genetically and phylogenetically distinct from the marine Thelohania (Clade V/Glugeida), whilst also describing two new species that give further support to the taxonomic revision. We propose that the freshwater Thelohania should be transferred to their own genus, Astathelohania gen. et comb. nov., in a new family (Astathelohaniidae n. fam.). This results in the revision of Thelohania contejeani (Astathelohania contejeani), Thelohania montirivulorum (Astathelohania montirivulorum), and Thelohania parastaci (Astathelohania parastaci). We also describe two novel muscle-infecting Astathelohania species, A. virili n. sp. and A. rusti n. sp., from North American crayfishes (Faxonius sp.). We used histological, molecular, and ultrastructural data to formally describe the novel isolates. Our data suggest that the Astathelohania are genetically distinct from other known microsporidian genera, outside any described family, and that their SSU rRNA gene sequence diversity follows their host species and native geographic location. The range of this genus currently includes North America, Europe, and Australia.