bluuebunny/crossref_metadata_2025 huggingface dataset:
Causes of Injury and Litigation in Cutaneous Laser Surgery: An Update From 2012 to 2020
Authors: Khalifian Saami , Vazirnia Aria , Mohan Girish C. , Thompson Kaitlyn V. , Jalian Hrak Ray , Avram Mathew M.
Published: January 2022

OBJECTIVE

To identify common causes of injury and liability claims related to cutaneous laser surgery from 2012 to 2020.

MATERIALS AND METHODS

Search of online national legal database of public legal documents regarding cutaneous laser surgery litigation.

RESULTS

From 2012 to 2020, 69 cases of liability claims due to a cutaneous laser surgery device were identified. Of these, 49 (71%) involved a nonphysician operator (NPO); 12 incidents (17%) involved non–core physician operators performing the procedure; 6 cases (9%) involved a plastic surgeon operator; and 2 cases (3%) involved a dermatologist operator. Laser hair removal was most litigated (44 cases, 64%), followed by laser skin rejuvenation (20 cases, 30%). Thirty-six of 69 cases had a discernible outcome, 53% (n = 19) rendered judgements in favor of the plaintiff, with a mean indemnity payment of $320,975 (range, $1,665–$1.5 million).

CONCLUSION

Previous work evaluating trends in laser surgery litigation from 1985 to 2012 identified increasing injury and legal action when performed by NPOs. Data from this study are consistent with these previous findings. Both studies demonstrate that NPOs account for most cases of legal action with an increasing proportion of cases being performed by NPOs. In this study, unsupervised NPOs comprise nearly three-quarters of laser surgery lawsuits, but the data may underestimate the frequency of injury and litigation caused by unsupervised NPOs.
Apixaban: Alternative Anticoagulation for HeartMate 3 Ventricular Assist Device
Authors: Whitehouse Katherine R. , Avula Divya , Kahlon Tanvir , Costelle Devan , Dunbar-Matos Christina , Pahwa Siddharth , Trivedi Jaimin R. , Slaughter Mark S.
Published: January 2022

Patients with left ventricular assist devices currently require long-term anticoagulation with warfarin. Warfarin requires frequent blood tests and is associated with adverse events when not in the therapeutic range. Apixaban is a possible alternative that is potentially better for compliance and requires no additional testing. The purpose of this study was to compare adverse events in patients with a HeartMate 3 LVAD receiving apixaban versus warfarin. Thirty-five patients underwent HM3 implantation between January 01, 2016 to January 31, 2021. The groups compared were apixaban (n = 15, 43%) and warfarin (n = 20, 57%). All patients received 325 mg aspirin daily. Stroke, bleeding, and death were identified as primary outcomes after LVAD implant. Univariate nonparametric statistical analysis was performed. The median duration of treatment with apixaban was 148 days (37–606 days). The groups were comparable in terms of age (56 vs. 54 years), gender (male, 85% vs. 75%), and renal function (Cr 1.5 vs. 1.4). The apixaban group had significantly higher mean pulmonary artery pressure (41 vs. 34, p = 0.03) and there were more (p < 0.05) ischemic cardiomyopathy and INTERMACS profile >3 in the warfarin group. At 6 months, thrombotic complications and death were not different between the groups. The two deaths in the apixaban group were from right heart failure. The apixaban group had clinically lower rates of bleeding complications (5% vs. 30%). The adverse events of bleeding, stroke, and death were similar in HM3 patients receiving warfarin or apixaban. Apixaban may be a safe alternative anticoagulant therapy in HM 3 LVAD patients.
Impaired antibody response to COVID-19 vaccination in advanced HIV infection
Authors: Hassold Nolan , Brichler Ségolène , Ouedraogo Elise , Leclerc Delphine , Carroue Sophie , Gater Yamina , Alloui Chakib , Carbonnelle Etienne , Bouchaud Olivier , Mechai Frederic , Cordel Hugues , Delagreverie Heloise
Published: January 2022

Objectives:

Coronavirus disease 2019 (COVID-19) vaccination is reportedly efficient in people with HIV (PWH) but vaccine trials included participants with normal CD4+ T-cell counts. We analyzed seroconversion rates and antibody titers following two-dose vaccination in PWH with impaired CD4+ T-cell counts.

Methods:

We collected retrospective postvaccination SARS-COV-2 serology results available in a university hospital for PWH vaccinated between March and September, 2021 who were tested for antispike antibodies from 8 to 150 days following dose 2. Antibody titers were compared in PWH with CD4+ T-cell count less than 200 cells/μl, 200 < CD4+ T-cell counts < 500 cells/μl and CD4+ T-cell count greater than 500 cells/μl at vaccination.

Results:

One hundred and five PWH were included: n = 54 in the CD4+ T-cell count less than 500 cells/μl group (n = 18 with CD4+ <200 cells/μl, n = 36 with 200 < CD4+ < 500 cells/μl) and 51 in the CD4+ T-cell count greater than 500 cells/μl group. They received two doses of BNT162b2 (75%), mRNA-1273 (8.5%), or ChAdOx1 nCoV-19 (16.5%). The median time from vaccine dose 2 to serology was consistent across all groups (73 days, interquartile range [29–97], P = 0.14). Seroconversion rates were 100% in the CD4+ T-cell count greater than 500 cells/μl group but 89% in participants with CD4+ T-cell counts less than 500 cells/μl (22 and 5.5% seronegative in the CD4+ T-cell counts <200 cells/μl and 200 < CD4+ < 500 cells/μl groups, respectively). Median antibody titers were 623.8 BAU/ml [262.2–2288] in the CD4+ greater than 500 cells/μl group versus 334.3 BAU/ml [69.9–933.9] in the CD4+ less than 500 cells/μl group (P = 0.003). They were lowest in the CD4+ less than 200 cells/μl group: 247.9 BAU/ml [5.88–434.9] (P = 0.0017) with only 44% achieving antibody titers above the putative protection threshold of 260 BAU/ml.

Conclusion:

PWH with CD4+ T-cell counts less than 500 cells/μl and notably less than 200 cells/μl had significantly lower seroconversion rates and antispike antibody titers compared with PWH with CD4+ T-cell counts greater than 500 cells/μl, warranting the consideration of targeted vaccine strategies in this fragile population.
Prevalence of nonalcoholic fatty liver disease using noninvasive techniques among children, adolescents, and youths living with HIV
Authors: Carrasco Itzíar , Olveira Antonio , Lancharro Ángel , Escosa Luis , Mellado María José , Busca Carmen , Montes María Luisa , Díez Cristina , Alcolea-Ruiz Sonia , Navarro María Luisa , Sainz Talía
Published: January 2022

Objective:

The prevalence of subclinical liver abnormalities is high among people with HIV, but data regarding perinatally HIV-infected children and adolescents (PHIV) are scarce. Noninvasive image techniques offer an opportunity to address nonalcoholic fatty liver disease (NAFLD) in a population in which the scores validated for adults have not been tested.

Design:

Prospective cross-sectional study including PHIV and uninfected controls.

Methods:

Noninvasive imaging techniques for the diagnosis of NAFLD and/or fibrosis were performed, and four scores to predict NAFLD were evaluated.

Results:

Seventy-six participants (59.2% women) with a median of 19 years old (interquartile range: 15.5–25.6) were included, 38 were PHIV and 38 were age and sex-matched controls. All HIV participants were on ART at the moment of inclusion, and 86.8% were virologically suppressed. A total of 11 PHIV and three controls were diagnosed with NAFLD (28.9% vs. 7.9%; P = 0.02) by noninvasive imaging techniques. The performance of scores based on clinical and analytical parameters was very poor. Although nonsignificant, overweight was more common among participants with NAFLD, who had a significantly higher BMI. Differences in HIV-related parameters between the groups were nonsignificant, except for the CD4+/CD8+ T-cells ratio, decreased among PHIV diagnosed with NAFLD (P = 0.04).

Conclusions:

The prevalence of NAFLD was high (28.9%) among PHIV, and only partially explained by overweight and metabolic syndrome defining factors. The scores based on clinical and analytical parameters did not accurately identify participants at risk. Therefore, liver ultrasound assessment should be considered for the screening of NAFLD among PHIV in routine clinical practice.
Effects of Aging on Metabolic Characteristics of Human B Cells
Authors: Frasca Daniela , Pallikkuth Suresh , Pahwa Savita
Published: January 2022

Metabolic changes represent the most common sign of aging and lead to increased risk of developing diseases typical of old age. Age-associated metabolic changes, such as decreased insulin sensitivity, decreased mitochondrial function, and dysregulated nutrient uptake, fuel the low-grade chronic systemic inflammation, known as inflammaging, a leading cause of morbidity and mortality, linked to the development of several diseases of old age. How aging affects the metabolic phenotype of immune cells, and B cells in particular, is not well known and is under intensive investigation by several groups. In this study, we summarized the few published results linking intrinsic B-cell metabolism and B-cell function in different groups of young and elderly individuals: healthy, with type-2 diabetes mellitus, or with HIV infection. Although preliminary, these results suggest the intriguing possibility that metabolic pathways can represent potential novel therapeutic targets to reduce inflammaging and improve humoral immunity.
Diabetic foot ulcer risk assessment and prevention in patients with diabetes: a best practice implementation project
Authors: Yang Lixiao , Wu Yanni , Zhou Chunlan , Xie Cuihua , Jiang Ya , Wang Run , Ye Xiaoling
Published: January 2022

Introduction and aim:

Diabetic foot ulcer (DFU) is one of the most serious complications in patients with diabetes. Early identification of risk factors can prevent its occurrence and delay its progression. The aim of this project is to conduct an audit of DFU risk assessment protocols at a large tertiary hospital and evaluate the impact of any changes in compliance with the developed evidence-based best practice criteria.

Methods:

Preimplementation and postimplementation audits based on JBI's Practical Application of Clinical Evidence System were conducted at the Department of Endocrinology and Metabolism of a tertiary hospital in China. The Getting Research into Practice audit tool was used to analyze the barriers and inadequacies encountered in practice. A total of 12 nurses and 30 patients with diabetes were included in the baseline and follow-up audits.

Results:

There were 15 baseline audits that indicated deficits in DFU risk assessment by nurses, with 0% compliance for three criteria and 50% compliance or less for seven others. Strategies developed by the project team to address the identified barriers to compliance were adopted, leading to significant improvement in compliance with most criteria at the follow-up audit compared with baseline with 11 criteria achieving at least 90% compliance.

Conclusion:

The project showed that regular audits of foot ulcer risk assessment can help to identify barriers to their implementation. Advising patients of their risk status can support appropriate self-care practices. Further audits are needed to implement evidence-based practices in all aspects of diabetes patient care.
Germline predisposition to pediatric Ewing sarcoma is uniquely characterized by inherited pathogenic variants in DNA damage repair genes
Authors: Gillani Riaz , Camp Sabrina Y. , Han Seunghun , Jones Jill K. , O’Brien Schuyler , Young Erin L. , Hayes Lucy , Mitchell Gareth , Fowler Trent , Gusev Alexander , Kamihara Junne , Janeway Katherine A. , Schiffman Joshua D. , Crompton Brian D. , AlDubayan Saud H. , Van Allen Eliezer M.
Published: January 2022

More knowledge is needed around the role and importance of specific genes in germline predisposition to Ewing sarcoma to inform biological investigation and clinical practice. In this study, we evaluated the enrichment of pathogenic germline variants in Ewing sarcoma relative to other pediatric sarcoma subtypes, as well as patterns of inheritance of these variants. We carried out an ancestry-matched case-control analysis to screen for enrichment of pathogenic germline variants in 141 established cancer predisposition genes in 1138 individuals with pediatric sarcoma diagnoses (222 Ewing sarcoma cases) relative to identically processed cancer-free controls. Findings in Ewing sarcoma were validated with an additional cohort of 425 individuals, and 301 Ewing sarcoma parent-proband trios were analyzed for inheritance patterns of identified pathogenic variants. A distinct pattern of pathogenic germline variants was seen in Ewing sarcoma relative to other sarcoma subtypes.FANCCwas the only gene with an enrichment signal for heterozygous pathogenic variants in the discovery Ewing sarcoma cohort (OR 14.4, 95% CI 3.5 – 51.2, p = 0.002, FDR = 0.28). This enrichment inFANCCheterozygous pathogenic variants was seen again in the Ewing sarcoma validation cohort (OR 5.1, 95% CI 1.2 – 18.5, p = 0.03, single hypothesis), representing a broader importance of genes involved in DNA damage repair, which were also nominally enriched in Ewing sarcoma cases. Pathogenic variants in DNA damage repair genes were acquired through autosomal inheritance. Our study provides new insight into germline risk factors contributing to Ewing sarcoma pathogenesis.
Oncogenic role of a developmentally regulatedNTRK2splice variant
Authors: Pattwell Siobhan S. , Arora Sonali , Nuechterlein Nicholas , Zager Michael , Loeb Keith R. , Cimino Patrick J. , Holland Nikolas C. , Reche-Ley Noemi , Bolouri Hamid , Almiron Bonnin Damian A. , Szulzewsky Frank , Phadnis Vaishnavi V. , Ozawa Tatsuya , Wagner Michael J. , Haffner Michael C. , Cao Junyue , Shendure Jay , Holland Eric C.
Published: January 2022

Temporally-regulated alternative splicing choices are vital for proper development yet the wrong splice choice may be detrimental. Here we highlight a novel role for the neurotrophin receptor splice variant TrkB.T1 in neurodevelopment, embryogenesis, transformation, and oncogenesis across multiple tumor types in both humans and mice. TrkB.T1 is the predominantNTRK2isoform across embryonic organogenesis and forced over-expression of this embryonic pattern causes multiple solid and nonsolid tumors in mice in the context of tumor suppressor loss. TrkB.T1 also emerges the predominantNTRKisoform expressed in a wide range of adult and pediatric tumors, including those harboring TRK fusions. Affinity purification-mass spectrometry (AP-MS) proteomic analysis reveals TrkB.T1 has distinct interactors with known developmental and oncogenic signaling pathways such as Wnt, TGF-ß, Hedgehog, and Ras. From alterations in splicing factors to changes in gene expression, the discovery of isoform specific oncogenes with embryonic ancestry has the potential to shape the way we think about developmental systems and oncology.
The transcriptomic signature of physiological trade-offs caused by larval overcrowding in Drosophila melanogaster
Authors: Morimoto Juliano , Derous Davina , Wenzel Marius , Henry Youn , Colinet Hervé
Published: January 2022

Intraspecific competition at the larval stage is an important ecological factor affecting life-history, adaptation and evolutionary trajectory in holometabolous insects. However, the molecular pathways and physiological trade-offs underpinning these ecological processes are poorly characterised. We reared Drosophila melanogaster at three egg densities (5, 60 and 300 eggs/ml) and sequenced the transcriptomes of pooled third-instar larvae. We also examined emergence time, egg-to-adult viability, adult mass and adult sex-ratio at each density. Medium crowding had minor detrimental effects on adult phenotypes compared to low density and yielded 24 differentially expressed genes (DEGs) including several chitinase enzymes. In contrast, high crowding had substantial detrimental effects on adult phenotypes and yielded 2107 DEGs. Among these, upregulated gene sets were enriched in sugar, steroid and amino acid metabolism as well as DNA replication pathways, whereas downregulated gene sets were enriched in ABC transporters, Taurine, Toll/Imd signalling and P450 xenobiotics metabolism pathways. Overall, our findings show that larval overcrowding has a large consistent effect on several molecular pathways (i.e., core responses) with few pathways displaying density-specific regulation (i.e., idiosyncratic responses). This provides important insights into how holometabolous insects respond to intraspecific competition during development.
Evaluation of an optimized protocol and Illumina ARTIC V4 primer pool for sequencing of SARS-CoV-2 using COVIDSeq™ and DRAGEN™ COVID Lineage App workflow
Authors: Clark Cyndi R. , Hardison Matthew T. , Houdeshell Holly N. , Vest Alec C. , Whitlock Darcy A. , Skola Dylan D. , Koble Jeffrey S. , Oberholzer Michael , Schroth Gary P.
Published: January 2022

Next-Generation Sequencing based genomic surveillance has been widely implemented for identification and tracking of emerging SARS-CoV-2 variants to guide the Public Health response to the COVID-19 pandemic. Amplicon-based assays, such as the Illumina® COVIDSeq™ Test (RUO) and COVIDSeq Assay (RUO), enable scalable sequencing of SARS-CoV-2, leveraging V3 and V4 primer designs from the ARTIC community and DRAGEN™ COVID Lineage App analysis available on Illumina BaseSpace™. We report here a comparison of COVIDSeq performance for SARS-CoV-2 genome reporting using the ARTIC V3 based primer pool (including primers for human control genes) that is provided with the COVIDSeq kit versus the ARTIC V4 based Illumina COVIDSeq V4 primer pool, using an optimized protocol and DRAGEN COVID Lineage App analysis. The data indicates that both primer pools enable robust reporting of SARS-CoV-2 variants. The Illumina COVIDSeq V4 primer pool has superior performance for SARS-CoV-2 genome reporting, particularly in samples with low virus load, and is therefore the recommended primer pool for genomic surveillance of SARS-CoV-2 for research use using COVIDSeq.
A graph-embedded topic model enables characterization of diverse pain phenotypes among UK Biobank individuals
Authors: Wang Yuening , Benavides Rodrigo , Diatchenko Luda , Grant Audrey V. , Li Yue
Published: January 2022

Large biobank repositories of clinical conditions and medications data open opportunities to investigate the phenotypic disease network. To enable systematic investigation of entire structured phenomes, we present graph embedded topic model (GETM). Our contributions are two folds in terms of method and applications. On the methodology side, we offer two main contributions in GETM. First, to aid topic inference, we integrate existing biomedical knowledge graph information in the form of pre-trained graph embedding into the embedded topic model. Second, leveraging deep learning techniques, we developed a variational autoencoder framework to infer patient phenotypic mixture by modeling multi-modal discrete patient medical records. In particular, for interpretability, we use a linear decoder to simultaneously infer the bi-modal distributions of the disease conditions and medications. On the application side, we applied GETM to UK Biobank (UKB) self-reported clinical phenotype data, which contains 443 self-reported medical conditions and 802 self-reported medications for 457,461 individuals. Compared to existing methods, GETM demonstrates overall superior performance in imputing missing conditions and medications. Here, we focused on characterizing pain phenotypes recorded in the questionnaire of the UKB individuals. GETM accurately predicts the status of chronic musculoskeletal (CMK) pain, chronic pain by body-site, and non-specific chronic pain using past conditions and medications. Our analyses revealed not only the known pain-related topics but also the surprising predominance of medications and conditions in the cardiovascular category among the most predictive topics across chronic pain phenotypes.
Quantifying biological carbon pump pathways with a data-constrained mechanistic model ensemble approach
Authors: Stukel Michael R. , Décima Moira , Landry Michael R.
Published: January 2022

The ability to constrain the mechanisms that transport organic carbon into the deep ocean is complicated by the multiple physical, chemical, and ecological processes that intersect to create, transform, and transport particles in the ocean. In this manuscript we develop and parameterize a data-assimilative model of the multiple pathways of the biological carbon pump (NEMUROBCP). The mechanistic model is designed to represent sinking particle flux, active transport by vertically migrating zooplankton, and passive transport by subduction and vertical mixing, while also explicitly representing multiple biological and chemical properties measured directly in the field (including nutrients, phytoplankton and zooplankton taxa, carbon dioxide and oxygen, nitrogen isotopes, and 234Thorium). Using 30 different data types (including standing stock and rate measurements related to nutrients, phytoplankton, zooplankton, and non-living organic matter) from Lagrangian experiments conducted on 11 cruises from four ocean regions, we conduct an objective statistical parameterization of the model and generate one million different potential parameter sets that are used for ensemble model simulations. The model simulates in situ parameters that were assimilated (net primary production and gravitational particle flux) and parameters that were withheld (234Thorium and nitrogen isotopes) with reasonable accuracy. Model results show that gravitational flux of sinking particles and vertical mixing of organic matter from the surface ocean are more important biological pump pathways than active transport by vertically-migrating zooplankton. However, these processes are regionally variable, with sinking particles most important in oligotrophic areas of the Gulf of Mexico and California, sinking particles and vertical mixing roughly equivalent in productive regions of the CCE and the subtropical front in the Southern Ocean, and active transport an important contributor in the Eastern Tropical Pacific. We further find that mortality at depth is an important component of active transport when mesozooplankton biomasses are high, but that it is negligible in regions with low mesozooplankton biomass. Our results also highlight the high degree of uncertainty, particularly amongst mesozooplankton functional groups, that is derived from uncertainty in model parameters, with important implications from results that rely on non-ensemble model outputs. We also discuss the implications of our results for other data assimilation approaches.
Household secondary attack rates of SARS-CoV-2 by variant and vaccination status: an updated systematic review and meta-analysis
Authors: Madewell Zachary J. , Yang Yang , Longini Ira M. , Halloran M. Elizabeth , Dean Natalie E.
Published: January 2022

We previously reported a household secondary attack rate (SAR) for SARS-CoV-2 of 18.9% through June 17, 2021. To examine how emerging variants and increased vaccination have affected transmission rates, we searched PubMed from June 18, 2021, through January 7, 2022. Meta-analyses used generalized linear mixed models to obtain SAR estimates and 95%CI, disaggregated by several covariates. SARs were used to estimate vaccine effectiveness based on the transmission probability for susceptibility (VES,p), infectiousness (VEI,p), and total vaccine effectiveness (VET,p). Household SAR for 27 studies with midpoints in 2021 was 35.8% (95%CI, 30.6%-41.3%), compared to 15.7% (95%CI, 13.3%-18.4%) for 62 studies with midpoints through April 2020. Household SARs were 38.0% (95%CI, 36.0%-40.0%), 30.8% (95%CI, 23.5%-39.3%), and 22.5% (95%CI, 18.6%-26.8%) for Alpha, Delta, and Beta, respectively.VEI,p,VES,p, andVET,pwere 56.6% (95%CI, 28.7%-73.6%), 70.3% (95%CI, 59.3%-78.4%), and 86.8% (95%CI, 76.7%-92.5%) for full vaccination, and 27.5% (95%CI, -6.4%-50.7%), 43.9% (95%CI, 21.8%-59.7%), and 59.9% (95%CI, 34.4%-75.5%) for partial vaccination, respectively. Household contacts exposed to Alpha or Delta are at increased risk of infection compared to the original wild-type strain. Vaccination reduced susceptibility to infection and transmission to others.

Household secondary attack rates (SARs) were higher for Alpha and Delta variants than previous estimates. SARs were higher to unvaccinated contacts than to partially or fully vaccinated contacts and were higher from unvaccinated index cases than from fully vaccinated index cases.
Fatty acids produced by the gut microbiota dampen host inflammatory responses by modulating intestinal SUMOylation
Authors: Ezzine Chaima , Loison Léa , Bole-Feysot Christine , Déchelotte Pierre , Coëffier Moïse , Ribet David
Published: January 2022

The gut microbiota produces a wide variety of metabolites, which interact with intestinal cells and contribute to host physiology. These metabolites regulate intestinal cell activities by modulating either gene transcription or post-translational modifications of gut proteins. The effect of gut commensal bacteria on SUMOylation, an essential ubiquitin-like modification in intestinal physiology, remains however unknown. Here, we show that short chain fatty acids (SCFAs) and branched chain fatty acids (BCFAs) produced by the gut microbiota increase protein SUMOylation in different intestinal cell lines in a pH-dependent manner. We demonstrate that these metabolites induce an oxidative stress which inactivates intestinal deSUMOylases and promotes the hyperSUMOylation of chromatin-bound proteins. In order to determine the impact of these modifications on intestinal physiology, we focused on the NF-κB signaling pathway, a key player in inflammation known to be regulated by SUMOylation. We demonstrated that the hyperSUMOylation induced by SCFAs/BCFAs inhibits the activation of the NF-κB pathway in intestinal cells by blocking the degradation of the inhibitory factor IκBα in response to TNFα. This results in a decrease in pro-inflammatory cytokines expression, such as IL8 or CCL20, as well as a decrease in intestinal epithelial permeability in response to TNFα. Together, our results reveal that fatty acids produced by gut commensal bacteria regulate intestinal physiology by modulating SUMOylation and illustrate a new mechanism of dampening of host inflammatory responses by the gut microbiota.
IMU-derived kinematics detect gait differences with age or knee osteoarthritis but differ from marker-derived inverse kinematics
Authors: Hafer Jocelyn F. , Mihy Julien A. , Hunt Andrew , Zernicke Ronald F. , Johnson Russell T.
Published: January 2022

Common in-lab, marker-based gait analyses may not represent daily, real-world gait. Real-world gait analyses may be feasible using inertial measurement units (IMUs), especially with recent advancements in open-source methods (e.g., OpenSense). Before using OpenSense to study real-world gait, we must determine whether these methods: (1) estimate joint kinematics similarly to traditional marker-based motion capture (MoCap) and (2) differentiate groups with clinically different gait mechanics. Healthy young and older adults and older adults with knee osteoarthritis completed this study. We captured MoCap and IMU data during overground walking at participants’ self-selected and faster speeds. MoCap and IMU kinematics were computed with appropriate OpenSim workflows. We tested whether sagittal kinematics differed between MoCap- and IMU-derived data, whether tools detected between-group differences similarly, and whether kinematics differed between tools by speed. MoCap data showed more flexion than IMU data (hip: 0-47 and 65-100% stride, knee: 0-38 and 58-91% stride, ankle: 18-100% stride). Group kinematics differed at the hip (young extension > knee osteoarthritis at 30-47% stride) and ankle (young plantar flexion > older healthy at 62-65% stride). Group-by-tool interactions occurred at the hip (61-63% stride). Significant tool-by-speed interactions were found, with hip and knee flexion increasing more for MoCap than IMU data with speed (hip: 12-15% stride, knee: 60-63% stride). While MoCap- and IMU-derived kinematics differed, our results suggested that the tools similarly detected clinically meaningful differences in gait. Results of the current study suggest that IMU-derived kinematics with OpenSense may enable the valid and reliable evaluation of gait in real-world, unobserved settings.
Purification of synchronized E. coli transcription elongation complexes by reversible immobilization on magnetic beads
Authors: Kelly Skyler L. , Szyjka Courtney E. , Strobel Eric J.
Published: January 2022

Synchronized transcription elongation complexes (TECs) are a fundamental tool for in vitro studies of transcription and RNA folding. Transcription elongation can be synchronized by omitting one or more NTPs from an in vitro transcription reaction so that RNA polymerase can only transcribe to the first occurrence of the omitted nucleotide(s) in the coding DNA strand. This approach was developed over four decades ago and has been applied extensively in biochemical investigations of RNA polymerase enzymes, but has not been optimized for RNA-centric assays. In this work, we describe the development of a system for isolating synchronized TECs from an in vitro transcription reaction. Our approach uses a custom 5’ leader sequence, called C3-SC1, to reversibly capture synchronized TECs on magnetic beads. We first show that complexes isolated by this procedure, called C3-SC1TECs, are >95% pure, >98% active, highly synchronous (94% of complexes chase in <15s upon addition of saturating NTPs), and compatible with solid-phase transcription; the yield of this purification is ∼8%. We then show that C3-SC1TECs perturb, but do not interfere with, the function of ZTP-sensing and ppGpp-sensing transcriptional riboswitches. For both riboswitches, transcription using C3-SC1TECs improved the efficiency of transcription termination in the absence of ligand but did not inhibit ligand-induced transcription antitermination. Given these properties, C3-SC1TECs will likely be useful for developing biochemical and biophysical RNA assays that require high-performance, quantitative bacterial in vitro transcription.
Monomodular Pseudomonas aeruginosa phage JG004 lysozyme (Pae87) contains a bacterial surface-active antimicrobial peptide-like region and a possible substrate-binding subdomain
Authors: Vázquez Roberto , Seoane-Blanco Mateo , Rivero-Buceta Virginia , Ruiz Susana , van Raaij Mark J. , García Pedro
Published: January 2022

Phage lysins are a source of novel antimicrobials to tackle the bacterial antibiotic resistance crisis. The engineering of phage lysins is being explored as a game-changing technological strategy for introducing a more precise approach in the way we apply antimicrobial therapy. Such engineering efforts will benefit from a better understanding of lysin structure and function. In this work, the antimicrobial activity of the endolysin from Pseudomonas aeruginosa phage JG004, termed Pae87, has been characterized. This lysin had been previously identified as an antimicrobial agent candidate, able to interact with the Gram-negative surface and disrupt it. Further evidence is hereby provided on this matter, based on a structural and biochemical study. A high-resolution crystal structure of Pae87 complexed with a peptidoglycan fragment showed a separate substrate-binding region within the catalytic domain, 18 Å away from the catalytic site and located at the opposite side of the lysin molecule. This substrate binding region was conserved among phylogenetically related lysins lacking an additional cell wall binding domain, but not among those containing such a module. Two glutamic acids were identified as relevant for the peptidoglycan degradation activity, although Pae87 antimicrobial activity was seemingly unrelated to it. In contrast, an antimicrobial peptide-like region within Pae87 C-terminus, named P87, was found to be able to actively disturb the outer membrane and have antibacterial activity by itself. Therefore, we propose an antimicrobial mechanism for Pae87 in which the P87 peptide plays the role of binding to the outer membrane and disrupting the cell wall function, either with or without the participation of Pae87 catalytic activity.

Synopsis

The structure of the monomodular Pseudomonas aeruginosa bacteriophage JG004 lysin Pae87 is presented and investigated in relationship with its function repurposed as an antimicrobial agent. The structure with its peptidoglycan ligand revealed a possible cell wall binding region. A C-terminal antimicrobial peptide-like region is shown to be important for disrupting the bacterial cell wall.
Prune intake ameliorates chronic constipation symptoms and causes little discomfort from diarrhea and loose stools: A randomized placebo-controlled trial
Authors: Koyama Taishi , Nagata Naoyoshi , Nishiura Kengo , Miura Naoki , Kawai Takashi , Yamamoto Hirotaka
Published: January 2022

Aim

Little data is available regarding the effectiveness of natural foods in treating chronic constipation. We aimed to identify whether prune ameliorates chronic constipation and can be used safely for a relatively long time.

Methods

In this double-blind, randomized, placebo-controlled trial, 84 subjects with chronic constipation, presenting more than 6 months before and persisting for more than 3 months, were randomized to prune (n=42) or placebo (n=42) intake for 8 weeks. We assessed daily Bristol stool form scale (BSFS) scores and stool frequencies and administered the gastrointestinal symptom rating scale (GSRS) questionnaire, as primary outcomes for constipation improvement.

Results

The prune group showed significantly decreased rates of hard stool (BSFS1 or 2) and increased rates of normal stool (BSFS 3 or 4) after 1 week, which were more evident after 7 weeks between the two groups. Prune significantly increased stool frequency immediately after 1 week. Furthermore, GSRS of hard stools, flatulence, and incomplete evacuation significantly improved after 4-8 weeks of prune intake, of which constipation and hard stools were significantly reduced compared to the placebo group. In contrast, prune intake did not cause diarrhea, loose stools, or urgent need for defecation during 8 weeks evaluated by GSRS score. We found no abnormal laboratory tests of liver function, renal function, inflammation, or urinalysis after prune intake.

Conclusions

Daily prune intake ameliorates chronic constipation, improving quality of life, and causes few diarrhea-related symptoms or side effects. Our results emphasize a new, useful, and easy strategy for chronic constipation. (UMIN ID:000041384)
Healthcare workers’ views on mandatory SARS-CoV-2 vaccination in the United Kingdom: findings from the UK-REACH prospective longitudinal cohort study
Authors: Woolf Katherine , Gogoi Mayuri , Martin Christopher A , Papineni Padmasayee , Lagrata Susie , Nellums Laura B , McManus I Chris , Guyatt Anna L , Melbourne Carl , Bryant Luke , Gupta Amit , John Catherine , Carr Sue , Tobin Martin D , Simpson Sandra , Gregary Bindu , Aujayeb Avinash , Zingwe Stephen , Reza Rubina , Gray Laura J , Khunti Kamlesh , Pareek Manish
Published: January 2022

Background

Several countries now have mandatory SARS-CoV-2/COVID-19 vaccination for healthcare workers (HCWs) or the general population. HCWs’ views on this are largely unknown.

Methods

We administered an online questionnaire to 17891 United Kingdom (UK) HCWs in Spring 2021 as part of the United Kingdom Research study into Ethnicity And COVID-19 outcomes in Healthcare workers (UK-REACH) nationwide prospective cohort study. We categorised responses to a free-text question “What should society do if people don’t get vaccinated against COVID-19?” using content analysis. We collapsed categories into a binary variable: favours mandatory vaccination or not and used logistic regression to calculate its demographic predictors, and occupational, health and attitudinal predictors adjusted for demographics.

Findings

Of 5633 questionnaire respondents, 3235 answered the freetext question; 18% (n=578) of those favoured mandatory vaccination but the most frequent suggestion was education (32%, n=1047). Older HCWs, HCWs vaccinated against influenza (OR 1.48; 95%CI 1.10 – 1.99, vs none) and with more positive vaccination attitudes generally (OR 1.10; 95%CI 1.06 – 1.14) were more likely to favour mandatory vaccination (OR 1.26; 95%CI 1.17 – 1.37, per decade increase), whereas female HCWs (OR= 0.80, 95%CI 0.65 – 0.99, vs male), Black HCWs (OR= 0.48, 95%CI 0.26 – 0.87, vs White), those hesitant about COVID-19 vaccination (OR= 0.56; 95%CI 0.43 – 0.71, vs not hesitant), in an Allied Health Profession (OR 0.67; 95%CI 0.51 – 0.88, vs Medical), or who trusted their organisation (OR 0.78; 95%CI 0.63 – 0.96) were less likely to.

Interpretation

Only one in six of the HCWs in this large, diverse, UK-wide sample favoured mandatory vaccination. Building trust, educating and supporting HCWs who are hesitant about vaccination may be more acceptable, effective and equitable.

Funding

MRC-UK Research and Innovation grant (MR/V027549/1) and the Department of Health and Social Care via the National Institute for Health Research.
Fourier coefficients for Laguerre–Sobolev type orthogonal polynomials
Authors: Molano Alejandro
Published: January 2022

Purpose

In this paper, the authors take the first step in the study of constructive methods by using Sobolev polynomials.

Design/methodology/approach

To do that, the authors use the connection formulas between Sobolev polynomials and classical Laguerre polynomials, as well as the well-known Fourier coefficients for these latter.

Findings

Then, the authors compute explicit formulas for the Fourier coefficients of some families of Laguerre–Sobolev type orthogonal polynomials over a finite interval. The authors also describe an oscillatory region in each case as a reasonable choice for approximation purposes.

Originality/value

In order to take the first step in the study of constructive methods by using Sobolev polynomials, this paper deals with Fourier coefficients for certain families of polynomials orthogonal with respect to the Sobolev type inner product. As far as the authors know, this particular problem has not been addressed in the existing literature.
Do religion and politics impact corporate governance diversity policy?
Authors: Gupta Parveen P. , Lam Kevin C.K. , Sami Heibatollah , Zhou Haiyan
Published: January 2022

Purpose

In this paper, the authors examine how religious and political factors affect a firm's corporate governance diversity policies.

Design/methodology/approach

The authors develop five basic empirical models. Model 1 examines how religious beliefs and political affiliation determine whether a firm will establish diversity incentive in its senior executives' performance assessment. Model 2 investigates how the diversity goal, religious beliefs and political affiliation separately affect the level of actual diversity achieved. Model 3 examines how the diversity goal and environmental factors interact to affect the level of actual diversity achieved. Model 4 and Model 5 examine whether the diversity incentive in senior executives' compensation plan and the environmental factors (religious belief and political affiliation) help to reduce the compensation differentials between male and female executives.

Findings

The authors find that firms located in more liberal counties with more Mainline Protestants and less Republican voters in the United States are more likely to include workforce diversity as a criterion in evaluating their senior executives. The authors also provide evidence that firms with diversity goals have more female directors, more female senior executives and more minority directors. However, they find no evidence that the compensation differentials between male and female executives are smaller in these firms. Finally, they find that external environment affects the effectiveness of the implementation of the diversity goals.

Originality/value

In line withthis branch of research, the authors expand the literate on the link between corporate culture and corporate decision-making by investigating the non-financial performance measures. Besides the corporate decision-making in investment, financial reporting and social responsibilities as documented in prior studies, the authors argue that the religious beliefs and political affiliations could also affect the development and implementation of corporate non-financial performance goals in executive incentive contracts.
Intelligent obstacle avoidance path planning method for picking manipulator combined with artificial potential field method
Authors: Fang Zheng , Liang Xifeng
Published: January 2022

Purpose

The results of obstacle avoidance path planning for the manipulator using artificial potential field (APF) method contain a large number of path nodes, which reduce the efficiency of manipulators. This paper aims to propose a new intelligent obstacle avoidance path planning method for picking robot to improve the efficiency of manipulators.

Design/methodology/approach

To improve the efficiency of the robot, this paper proposes a new intelligent obstacle avoidance path planning method for picking robot. In this method, we present a snake-tongue algorithm based on slope-type potential field and combine the snake-tongue algorithm with genetic algorithm (GA) and reinforcement learning (RL) to reduce the path length and the number of path nodes in the path planning results.

Findings

Simulation experiments were conducted with tomato string picking manipulator. The results showed that the path length is reduced from 4.1 to 2.979 m, the number of nodes is reduced from 31 to 3 and the working time of the robot is reduced from 87.35 to 37.12 s, after APF method combined with GA and RL.

Originality/value

This paper proposes a new improved method of APF, and combines it with GA and RL. The experimental results show that the new intelligent obstacle avoidance path planning method proposed in this paper is beneficial to improve the efficiency of the robotic arm.

Graphical abstract

Figure 1 According to principles of bionics, we propose a new path search method, snake-tongue algorithm, based on a slope-type potential field. At the same time, we use genetic algorithm to strengthen the ability of the artificial potential field method for path searching, so that it can complete the path searching in a variety of complex obstacle distribution situations with shorter path searching results. Reinforcement learning is used to reduce the number of path nodes, which is good for improving the efficiency of robot work. The use of genetic algorithm and reinforcement learning lays the foundation for intelligent control.
Is conditional conservatism a source of deviations of financial statements from Benford's Law?
Authors: Nguyen Tri Tri , Duong Chau Minh , Nguyen Nguyet Thi Minh
Published: January 2022

Purpose

In this paper, the authors examine the association between conditional conservatism and deviations of the first digits of financial statement items from what are expected by Benford's Law.

Design/methodology/approach

This research uses data of companies listed on the London Stock Exchange. The authors measure deviations of first digits from Benford's Law following Amiramet al. (2015) and firm-year conditional conservatism following previous studies (Basu, 1997; Khan and Watts, 2009; García Laraet al., 2016). The authors use multiple regressions to provide evidence for their hypothesis.

Findings

The results show that conditional conservatism is positively associated with deviations from Benford's Law. The findings are robust across different measures of deviations and conditional conservatism. Also, the authors find that the relationship between deviations from Benford's Law and conditional conservatism is more pronounced for firms with debt issuance, and for leveraged firms facing financial distress. Next, the authors’ analyses confirm previous evidence by showing that the first digits of financial statement items of UK listed companies conform to Benford's Law at the firm-specific level and the market level, and deviations of income statements are larger than those of balance sheets and cash flow statements.

Research limitations/implications

The research makes significant contributions to the literature. First, this is the first study that provides empirical evidence suggesting that conditional conservatism may be a source of deviations from Benford’s Law. Second, the authors provide evidence confirming previous US findings (e.g. Amiramet al., 2015) showing that the distributions of first digits of financial statement items of UK listed companies also conform to Benford's Law.

Practical implications

The authors’ findings have implications for auditors. Auditors should be aware of “false positive” for material misstatements when using Benford's Law as a risk assessment procedure. While both conditional conservatism and earnings management are related to deviations from Benford's Law, conservatism-related biases could indicate less audit risks.

Originality/value

The authors provide new and original evidence suggesting that conditional conservatism is related to deviations from Benford's Law.
Evaluate the sustainable marketing strategy to optimal online leasing of new energy vehicles under the background big data economy
Authors: Daifen Tang
Published: January 2022

Purpose

Under the big data background, there are many influencing factors for investors of new energy vehicles (NEV), and government subsidies promote the sustainable development of the new energy vehicle industry. Therefore, the purpose of the study is to provide solutions for the sustainable development of NEV.

Design/methodology/approach

The sustainable marketing strategy of NEV in China is put forward. This paper first analyzes the subsidy policy effect of NEV under the background of big data. It then establishes the online optimal leasing strategy under multiple strategy choices and the online leasing strategy of multiple vehicles under the inflation market.

Findings

With the fixed cost of NEV in each lease period, the optimal competition ratio of online decision-makers will continue to decrease with the increase of the difference between prepaid funds and government subsidies. In the decision-making of renting and purchasing multiple vehicles, the general strategy competition ratio is 2.922, while the optimal competition ratio of the online renting and purchasing strategy proposed by the research is 2.723.

Research limitations/implications

The research is limited by the limited data and information collected, so the optimal decision-making model has some limitations. The authors need to find more representative data to optimize the model.

Practical implications

As an emerging industry, NEV have developed rapidly in recent years. Based on the online algorithm and competitive ratio theory, this paper solves the decision-making problem of operators and gives the optimal strategy to promote the green development of the new energy vehicle industry.

Originality/value

This paper proposes the optimal strategy for online investors of new energy vehicle operators by combining online algorithm and competitive ratio theory. The numerical analysis results of the optimal online model under multi strategy selection show that with the same difference between prepaid funds and government subsidies, the time point will be delayed and the time point will be advanced as the cost of leasing NEV in each period increases.