bluuebunny/crossref_metadata_2025 huggingface dataset:
Lunate-capitate arthrodesis for scaphoid nonunion: a comparative study
Authors: Elshahhat Amr , Abed Yaser , Nour Khaled
Published: August 2024

Background

Scaphoid nonunion advanced collapse (SNAC) injuries are frequently associated with irreversible degenerative wrist arthritic changes that necessitate surgical intervention. Midcarpal fusion remains the mainstay of the management of SNAC II and III injuries. A successful four-corner fusion (4CF) relies on a stable lunate-capitate fusion (LCF). There have been reports of management relying solely on LCF. The outcomes of LC- and 4 C-fusions in SNAC injuries were not widely documented. The objective of this research is to provide valuable insights into the effectiveness of both fusion procedures in the management of SNAC II and III wrist injuries, with a focus on reporting associated complications, functional and radiological outcomes.

Patients and methods

This retrospective study encompassed 65 patients diagnosed with SNAC II and III wrist injuries who underwent limited wrist fusion procedures between 2015 and 2024, with a minimum of 2 years of postoperative follow-up. Exclusion criteria encompassed patients with carpal instability, prior wrist surgical interventions, and scapholunate advanced collapse. Following the fusion procedure performed, patients were stratified into two groups: the LCF group consisting of 31 patients, and the 4CF group comprising 34 patients. Preoperative and intraoperative data were retrieved from the patient’s medical records. At their final follow-up appointments, patients underwent comprehensive radiographic and clinical evaluations. Clinical outcomes including hand grip strength, range of motion, the Disabilities of the Arm, Shoulder, and Hand Score, and the Mayo Modified Wrist Score, were compared between groups. Any associated complications were reported.

Results

The average healing time was 74.7 ± 15.6 and 72.2 ± 13.2 days for the LCF and 4CF groups, respectively. At the final visit, all patients showed functional improvement relative to their preoperative status, with comparable wrist range of motions observed in both groups. The functional wrist scores were slightly better in the LCF patients (P > 0.05). The average grip strength was significantly greater in the LCF group (P = 0.04), with mean strength values of 86.8% and 82.1% of the contralateral side, for the LCF and 4CF groups, respectively.

Conclusion

The LCF is not less efficient than the 4CF in the treatment of SNAC II and III wrist injuries. Through a less time-consuming procedure, LCF can efficiently provide comparable results to 4CF.

Level of evidence

level IV evidence.
The impact of aging and thickness on flexural strength of various zirconia ceramics
Authors: Silmeoglu Yagli Oyku , Talay Cevlik Esra , Kurklu Arpacay Duygu
Published: August 2024

Background

Effects of the aging process on the flexural strength of Y-TZP and different Y-PSZ ceramics of different thicknesses were investigated.

Methods

300 disc-shaped samples (12 mm diameter, 0.8 and 1.5 mm thicknesses) were made from 5 different zirconia materials 3Y-TZP LA, 4Y-PSZ, 5Y-PSZ, 3 + 5Y-PSZ and 4 + 5Y-PSZ. Experimental groups were artificially aged in an autoclave at 134 °C, 2 bar pressure for 1 and 5 h; control groups were not subjected to any treatment. Microstructural analysis was conducted using Scanning Electron Microscopy, and X-Ray Diffraction analysis determined the crystalline phase content. The impact of aging on flexural strength was investigated with the use of the biaxial flexural strength test. Data were analyzed using three-way ANOVA tests with a significance level of p < 0.05, applying Bonferroni correction for multiple comparisons.

Results

Statistically significant differences in flexural strength were observed among the materials and the material thicknesses (p < 0.05), while there were no significant differences among the aging times (p > 0.05). The highest mean flexural strength values were recorded in the case of the 3 Y-TZP-1.5 mm–5 h group (744.1 ± 61.2 MPa), which was attributed to phase-transformation toughening. The lowest values were observed in the case of the 5 Y-PSZ-1.5 mm–5 h (338.3 ± 34.8 MPa) group.

Conclusions

Both material type and thickness significantly affect the flexural strength of zirconia ceramics, whereas aging time does not; thus, material selection and thickness are crucial considerations for clinicians.
Threats of nursing productivity in the digital era: investigating the interplay between smartphones addiction and procrastination behavior among nurses
Authors: El-Sayed Ahmed Abdelwahab Ibrahim , Goda Shimaa Fathy Daif Allah , Elbialy Gehan Galal
Published: August 2024

Background

Controlling smartphone addiction and procrastination among nurses is crucial for enhancing the productivity of both nursing and the healthcare system. Critical care nurses are highly vulnerable to smartphone addiction and procrastination behaviors than other groups. They may purposefully delay their tasks, a practice known as active procrastination, or inadvertently delay them, a practice known as passive procrastination.

Aim

This study was designed to assess the prevalence of smartphone addiction and procrastination behavior among nurses, examine the effect of smartphone addiction on the active and passive procrastination behaviors, and explore the correlation between active and passive procrastination behaviors among nurses.

Method

This is a descriptive correlational exploratory study that was conducted at 23 critical care units of one large educational hospital in Egypt. Data were collected from 360 nurses who were conveniently selected using three tools: the Smartphone Addiction Inventory, the New Active Procrastination Scale, and the Unintentional Procrastination Scale. Correlation and regression analyses were conducted to test the hypothetical relationship among the study variables.

Results

This study revealed that 55.0%, 80.0%, and 45.3% of nurses had a moderate perceived level of smartphone addiction, active procrastination behavior, and passive procrastination behavior, respectively. There is a significant positive correlation between smartphone addiction and both nurses’ active and passive procrastination behaviors. Smartphone addiction accounts for 25% of the variance in nurses’ active procrastination behavior and 18% of the variance in their passive procrastination. Furthermore, there is a moderately significant negative correlation between nurses’ active procrastination behavior and their passive procrastination behavior.

Conclusion

Nurses are exhibiting moderate levels of smartphone addiction and procrastination, which is a significant threat to the healthcare industry and nursing productivity. This requires technological, educational, and organizational interventions that foster active procrastination and combat passive procrastination behaviors among nurses.

Implications

Continuous training programs are required to enhance time management skills among nurses and increase the awareness of nurse managers with the symptoms of smartphone addiction among nurses. Nurse leaders should early detect and address the addictive use of smartphones among nurses, identify potential procrastinators, and provide counseling to eradicate these behaviors in the workplace.
Assessment of multi-professional primary healthcare center quality by patients with multimorbidity
Authors: Dany Antoine , Aujoulat Paul , Colin Floriane , Le Reste Jean-Yves , Le Goff Delphine
Published: August 2024

Background

The main aim of this study was to build an item bank for assessing the care quality of multi-professional healthcare centers (MPHCC) from the perspective of patients with multimorbidity. This study was part of the QUALSOPRIM (QUALité des SOins PRIMaires; primary healthcare quality) research project to create a psychometrically robust self-administered questionnaire to assess healthcare quality.

Methods

First, twelve experts built an item bank using data from a previous qualitative work and a systematic literature review. Second, the validity of each item was assessed in a sample of patients. Adult patients with multimorbidity were recruited from six French MPHCC. Items were assessed based on ceiling effects, the level of missing or neutral responses and patient feedback. Patient feedback was recorded after the item bank completion. Based on results, items were validated, improved, or removed during expert meetings. In case of disagreement the Delphi method was used to reach consensus.

Results

The study sample included 209 outpatients. The most frequent medical conditions were cardiovascular risk factors, cardiovascular diseases and rheumatological conditions. In total, a bank of 109 items classified in nine domains was built. The validity assessment led to the removal of 34 items. Retained items explored a variety of topics related to care quality: availability, accessibility, premises’ layout and building, technical care, expertise, organization, relationships with caregivers and communication, involvement and personal relationships.

Conclusions

This study allowed cross-validation of a bank of 75 items, leading to a complete picture of the patient perception of care quality items. Overall, patients were generally satisfied with their care at the MPHCC. Nonetheless, there were still numerous items on subjects for which patients’ satisfaction could be improved.
Perceptions of hospital electronic health record (EHR) training, support, and patient safety by staff position and tenure
Authors: Campione Joanne , Liu Helen
Published: August 2024

Background

Hospitals rely on their electronic health record (EHR) systems to assist with the provision of safe, high quality, and efficient health care. However, EHR systems have been found to disrupt clinical workflows and may lead to unintended consequences associated with patient safety and health care professionals’ perceptions of and burden with EHR usability and interoperability. This study sought to explore the differences in staff perceptions of the usability and safety of their hospital EHR system by staff position and tenure.

Methods

We used data from the AHRQ Surveys on Patient Safety Culture® (SOPS®) Hospital Survey Version 1.0 Database and the SOPS Health Information Technology Patient Safety Supplemental Items (“Health IT item set”) collected from 44 hospitals and 8,880 staff in 2017. We used regression modeling to examine perceptions of EHR system training, EHR support & communication, EHR-related workflow, satisfaction with the EHR system, and the frequency of EHR-related patient safety and quality issues by staff position and tenure, while controlling for hospital ownership type and bed-size.

Results

In comparison to RNs, pharmacists had significantly lower (unfavorable) scores for EHR system training (regression coefficient = -0.07; p = 0.047), and physicians, hospital management, and the IT staff were significantly more likely to report high frequency of inaccurate EHR information (ORs = 2.03, 1.34, 1.72, respectively). Compared to staff with 11 or more years of hospital tenure, new staff (less than 1 year at the hospital) had significantly lower scores for EHR system training, but higher scores for EHR support & communication (p < 0.0001). Dissatisfaction of the EHR system was highest among physicians and among staff with 11 or more years tenure at the hospital.

Conclusions

There were significant differences in the Health IT item set’s results across staff positions and hospital tenure. Hospitals can implement the SOPS Health IT Patient Safety Supplemental Items as a valuable tool for identifying incongruity in the perceptions of EHR usability and satisfaction across staff groups to inform targeted investment in EHR system training and support.
Activation of the yeast Retrograde Response pathway by adaptive laboratory evolution with S-(2-aminoethyl)-L-cysteine reduces ethanol and increases glycerol during winemaking
Authors: Garrigós Víctor , Picazo Cecilia , Matallana Emilia , Aranda Agustín
Published: August 2024

Background

Global warming causes an increase in the levels of sugars in grapes and hence in ethanol after wine fermentation. Therefore, alcohol reduction is a major target in modern oenology. Deletion of the MKS1 gene, a negative regulator of the Retrograde Response pathway, in Saccharomyces cerevisiae was reported to increase glycerol and reduce ethanol and acetic acid in wine. This study aimed to obtain mutants with a phenotype similar to that of the MKS1 deletion strain by subjecting commercial S. cerevisiae wine strains to an adaptive laboratory evolution (ALE) experiment with the lysine toxic analogue S-(2-aminoethyl)-L-cysteine (AEC).

Results

In laboratory-scale wine fermentation, isolated AEC-resistant mutants overproduced glycerol and reduced acetic acid. In some cases, ethanol was also reduced. Whole-genome sequencing revealed point mutations in the Retrograde Response activator Rtg2 and in the homocitrate synthases Lys20 and Lys21. However, only mutations in Rtg2 were responsible for the overactivation of the Retrograde Response pathway and ethanol reduction during vinification. Finally, wine fermentation was scaled up in an experimental cellar for one evolved mutant to confirm laboratory-scale results, and any potential negative sensory impact was ruled out.

Conclusions

Overall, we have shown that hyperactivation of the Retrograde Response pathway by ALE with AEC is a valid approach for generating ready-to-use mutants with a desirable phenotype in winemaking.
Prediction accuracy and repeatability of UAV based biomass estimation in wheat variety trials as affected by variable type, modelling strategy and sampling location
Authors: Smith Daniel T. L. , Chen Qiaomin , Massey-Reed Sean Reynolds , Potgieter Andries B. , Chapman Scott C.
Published: August 2024

Background

This study explores the use of Unmanned Aerial Vehicles (UAVs) for estimating wheat biomass, focusing on the impact of phenotyping and analytical protocols in the context of late-stage variety selection programs. It emphasizes the importance of variable selection, model specificity, and sampling location within the experimental plot in predicting biomass, aiming to refine UAV-based estimation techniques for enhanced selection accuracy and throughput in variety testing programs.

Results

The research uncovered that integrating geometric and spectral traits led to an increase in prediction accuracy, whilst a recursive feature elimination (RFE) based variable selection workflowled to slight reductions in accuracy with the benefit of increased interpretability. Models, tailored to specific experiments were more accurate than those modelling all experiments together, while models trained for broad-growth stages did not significantly increase accuracy. The comparison between a permanent and a precise region of interest (ROI) within the plot showed negligible differences in biomass prediction accuracy, indicating the robustness of the approach across different sampling locations within the plot. Significant differences in the within-season repeatability (w2) of biomass predictions across different experiments highlighted the need for further investigation into the optimal timing of measurement for prediction.

Conclusions

The study highlights the promising potential of UAV technology in biomass prediction for wheat at a small plot scale. It suggests that the accuracy of biomass predictions can be significantly improved through optimizing analytical and modelling protocols (i.e., variable selection, algorithm selection, stage-specific model development). Future work should focus on exploring the applicability of these findings under a wider variety of conditions and from a more diverse set of genotypes.
Geniposide for treating atherosclerotic cardiovascular disease: a systematic review on its biological characteristics, pharmacology, pharmacokinetics, and toxicology
Authors: Li Dexiu , Li Xiaoya , Zhang Xiaonan , Chen Jiye , Wang Zeping , Yu Zongliang , Wu Min , Liu Longtao
Published: August 2024

In recent years, the prevalence and fatality rates of atherosclerotic cardiovascular disease have not only shown a consistent rise that cannot be ignored, but have also become a pressing social health problem that requires urgent attention. While interventional surgery and drug therapy offer significant therapeutic results, they often come with common side effects. Geniposide, an active component extracted from the Chinese medicine Gardenia jasminoides Ellis, shows promise in the management of cardiac conditions. This review comprehensively outlines the underlying pharmacological mechanisms by which geniposide exerts its effects on atherosclerosis. Geniposide exhibits a range of beneficial effects including alleviating inflammation, inhibiting the development of macrophage foam cells, improving lipid metabolism, and preventing platelet aggregation and thrombosis. It also demonstrates mitochondrial preservation, anti-apoptotic effects, and modulation of autophagy. Moreover, geniposide shows potential in improving oxidative stress and endoplasmic reticulum stress by maintaining the body’s antioxidant and oxidative balance. Additionally, this review comprehensively details the biological properties of geniposide, including methods of extraction and purification, as well as its pharmacokinetics and toxicological characteristics. It further discusses the clinical applications of related biopharmaceuticals, emphasizing the potential of geniposide in the prevention and treatment of atherosclerotic cardiovascular diseases. Furthermore, it highlights the limitations of current research, aiming to provide insights for future studies.

Graphical Abstract
Repurposed AT9283 triggers anti-tumoral effects by targeting MKK3 oncogenic functions in Colorectal Cancer
Authors: Piastra Valentina , Ganci Federica , Sacconi Andrea , Pranteda Angelina , Allegretti Matteo , Bernardini Roberta , Serra Martina , Lupo Barbara , Dell’Aquila Emanuela , Ferretti Gianluigi , Pescarmona Edoardo , Bartolazzi Armando , Blandino Giovanni , Trusolino Livio , Bossi Gianluca
Published: August 2024

Background

Colorectal cancer (CRC) is the third most common type of cancer and the second leading cause of cancer-related deaths worldwide, with a survival rate near to 10% when diagnosed at an advanced stage. Hence, the identification of new molecular targets to design more selective and efficient therapies is urgently required. The Mitogen activated protein kinase kinase 3 (MKK3) is a dual-specificity threonine/tyrosine protein kinase that, activated in response to cellular stress and inflammatory stimuli, regulates a plethora of biological processes. Previous studies revealed novel MKK3 roles in supporting tumor malignancy, as its depletion induces autophagy and cell death in cancer lines of different tumor types, including CRC. Therefore, MKK3 may represent an interesting new therapeutic target in advanced CRC, however selective MKK3 inhibitors are currently not available.

Methods

The study involved transcriptomic based drug repurposing approach and confirmatory assays with CRC lines, primary colonocytes and a subset of CRC patient-derived organoids (PDO). Investigations in vitro and in vivo were addressed.

Results

The repurposing approach identified the multitargeted kinase inhibitor AT9283 as a putative compound with MKK3 depletion-mimicking activities. Indeed, AT9283 drops phospho- and total-MKK3 protein levels in tested CRC models. Likely the MKK3 silencing, AT9283 treatment: i) inhibited cell proliferation promoting autophagy and cell death in tested CRC lines and PDOs; ii) resulted well-tolerated by CCD-18Co colonocytes; iii) reduced cancer cell motility inhibiting CRC cell migration and invasion; iv) inhibited COLO205 xenograft tumor growth. Mechanistically, AT9283 abrogated MKK3 protein levels mainly through the inhibition of aurora kinase A (AURKA), impacting on MKK3/AURKA protein–protein interaction and protein stability therefore uncovering the relevance of MKK3/AURKA crosstalk in sustaining CRC malignancy in vitro and in vivo.

Conclusion

Overall, we demonstrated that the anti-tumoral effects triggered by AT9283 treatment recapitulated the MKK3 depletion effects in all tested CRC models in vitro and in vivo, suggesting that AT9283 is a repurposed drug. According to its good tolerance when tested with primary colonocytes (CCD-18CO), AT9283 is a promising drug for the development of novel therapeutic strategies to target MKK3 oncogenic functions in late-stage and metastatic CRC patients.
Exposure-associated DNA methylation among people exposed to multiple industrial pollutants
Authors: Chen Chi-Hsin Sally , Yuan Tzu-Hsuen , Lu Tzu-Pin , Lee Hsin-Ying , Chen Yi-Hsuen , Lai Liang-Chuan , Tsai Mong-Hsun , Chuang Eric Y. , Chan Chang-Chuan
Published: August 2024

Background

Current research on the epigenetic repercussions of exposure to a combination of pollutants is limited. This study aims to discern DNA methylation probes associated with exposure to multiple pollutants, serving as early effect markers, and single-nucleotide polymorphisms (SNPs) as surrogate indicators for population susceptibility. The investigation involved the analysis of urine exposure biomarkers for 11 heavy metals (vanadium, arsenic, mercury, cadmium, chromium, nickel, lead, manganese, copper, strontium, thallium), polycyclic aromatic hydrocarbon (PAHs) (1-hydroxypyrene), genome-wide DNA methylation sequencing, and SNPs array on all study participants. The data were integrated with metabolomics information and analyzed both at a community level based on proximity to home addresses relative to the complex and at an individual level based on exposure biomarker concentrations.

Results

On a community level, 67 exposure-related CpG probes were identified, while 70 CpG probes were associated with urine arsenic concentration, 2 with mercury, and 46 with vanadium on an individual level. These probes were annotated to genes implicated in cancers and chronic kidney disease. Weighted quantile sum regression analysis revealed that vanadium, mercury, and 1-hydroxypyrene contributed the most to cg08238319 hypomethylation. cg08238319 is annotated to the aryl hydrocarbon receptor repressor (AHRR) gene, and AHRR hypomethylation was correlated with an elevated risk of lung cancer. AHRR was further linked to deregulations in phenylalanine metabolism, alanine, aspartate, and glutamate metabolism, along with heightened oxidative stress. Additionally, three SNPs (rs11085020, rs199442, and rs10947050) corresponding to exposure-related CpG probes exhibited significant interaction effects with multiple heavy metals and PAHs exposure, and have been implicated in cancer progression and respiratory diseases.

Conclusion

Our findings underscore the pivotal role of AHRR methylation in gene-environment interactions and highlight SNPs that could potentially serve as indicators of population susceptibility in regions exposed to multiple heavy metals and PAHs.
Early reapplication of prone position during venovenous ECMO for acute respiratory distress syndrome: a prospective observational study and propensity-matched analysis
Authors: Wang Rui , Tang Xiao , Li Xuyan , Li Ying , Liu Yalan , Li Ting , Zhao Yu , Wang Li , Li Haichao , Li Meng , Li Hu , Tong Zhaohui , Sun Bing
Published: August 2024

Background

A combination of prone positioning (PP) and venovenous extracorporeal membrane oxygenation (VV-ECMO) is safe, feasible, and associated with potentially improved survival for severe acute respiratory distress syndrome (ARDS). However, whether ARDS patients, especially non-COVID-19 patients, placed in PP before VV-ECMO should continue PP after a VV-ECMO connection is unknown. This study aimed to test the hypothesis that early use of PP during VV-ECMO could increase the proportion of patients successfully weaned from ECMO support in severe ARDS patients who received PP before ECMO.

Methods

In this prospective observational study, patients with severe ARDS who were treated with VV-ECMO were divided into two groups: the prone group and the supine group, based on whether early PP was combined with VV-ECMO. The proportion of patients successfully weaned from VV-ECMO and 60-day mortality were analyzed before and after propensity score matching.

Results

A total of 165 patients were enrolled, 50 in the prone and 115 in the supine group. Thirty-two (64%) and 61 (53%) patients were successfully weaned from ECMO in the prone and the supine groups, respectively. The proportion of patients successfully weaned from VV-ECMO in the prone group tended to be higher, albeit not statistically significant. During PP, there was a significant increase in partial pressure of arterial oxygen (PaO2) without a change in ventilator or ECMO settings. Tidal impedance shifted significantly to the dorsal region, and lung ultrasound scores significantly decreased in the anterior and posterior regions. Forty-five propensity score-matched patients were included in each group. In this matched sample, the prone group had a higher proportion of patients successfully weaned from VV-ECMO (64.4% vs. 42.2%; P = 0.035) and lower 60-day mortality (37.8% vs. 60.0%; P = 0.035).

Conclusions

Patients with severe ARDS placed in PP before VV-ECMO should continue PP after VV-ECMO support. This approach could increase the probability of successful weaning from VV-ECMO.

Trial Registration

ClinicalTrials.Gov: NCT04139733. Registered 23 October 2019.
Recovery of plant nutrients from human excreta and domestic wastewater for reuse in agriculture: a systematic map and evidence platform
Authors: Macura Biljana , Metson Geneviève S. , McConville Jennifer R. , Harder Robin
Published: August 2024

Background

Achieving a more circular and efficient use of nutrients found in human excreta and domestic (municipal) wastewater is an integral part of mitigating aquatic nutrient pollution and nutrient insecurity. A synthesis of research trends readily available to various stakeholders is much needed. This systematic map collates and summarizes scientific research on technologies that facilitate the recovery and reuse of plant nutrients and organic matter found in human excreta and domestic wastewater. We present evidence in a way that can be navigated easily. We hope this work will help with the uptake and upscaling of new and innovative circular solutions for the recovery and reuse of nutrients.

Methods

The systematic map consists of an extension of two previous related syntheses. Searches were performed in Scopus and Web of Science in English. Records were screened on title and abstract, including consistency checking. Coding and meta-data extraction included bibliographic information, as well as recovery pathways. The evidence from the systematic map is embedded in an online evidence platform that, in an interactive manner, allows stakeholders to visualize and explore the systematic map findings, including knowledge gaps and clusters.

Results

The evidence base includes a total of 10 950 articles describing 11 489 recovery pathways. Most of the evidence base is about recovery technologies (41.9%) and the reuse of recovered products in agriculture (53.4%). A small proportion of the evidence base focuses on the characteristics of recovered products (4.0%) and user acceptance and perceptions of nutrient recovery and reuse (0.7%).

Conclusions

Most studies we mapped focused on nutrient recovery from ‘conventional’ systems, that is, from centralized sewer and wastewater treatment systems that produce biosolids and a treated effluent. While we also found substantial research on nutrient recovery from source-separated urine, and to some extent also on nutrient recovery from source-separated excreta (notably blackwater), the body of research on nutrient recovery from source-separated feces was relatively small. Another knowledge gap is the relative lack of research on the recovery of potassium. More research is also needed on user acceptance of different recovery technologies and recovered products.
CO-driven electron and carbon flux fuels synergistic microbial reductive dechlorination
Authors: Wang Jingjing , Li Xiuying , Jin Huijuan , Yang Shujing , Yu Lian , Wang Hongyan , Huang Siqi , Liao Hengyi , Wang Xuhao , Yan Jun , Yang Yi
Published: August 2024

Background

Carbon monoxide (CO), hypothetically linked to prebiotic biosynthesis and possibly the origin of the life, emerges as a substantive growth substrate for numerous microorganisms. In anoxic environments, the coupling of CO oxidation with hydrogen (H2) production is an essential source of electrons, which can subsequently be utilized by hydrogenotrophic bacteria (e.g., organohalide-respring bacteria). While Dehalococcoides strains assume pivotal roles in the natural turnover of halogenated organics and the bioremediation of chlorinated ethenes, relying on external H2 as their electron donor and acetate as their carbon source, the synergistic dynamics within the anaerobic microbiome have received comparatively less scrutiny. This study delves into the intriguing prospect of CO serving as both the exclusive carbon source and electron donor, thereby supporting the reductive dechlorination of trichloroethene (TCE).

Results

The metabolic pathway involved anaerobic CO oxidation, specifically the Wood-Ljungdahl pathway, which produced H2 and acetate as primary metabolic products. In an intricate microbial interplay, these H2 and acetate were subsequently utilized by Dehalococcoides, facilitating the dechlorination of TCE. Notably, Acetobacterium emerged as one of the pivotal collaborators for Dehalococcoides, furnishing not only a crucial carbon source essential for its growth and proliferation but also providing a defense against CO inhibition.

Conclusions

This research expands our understanding of CO’s versatility as a microbial energy and carbon source and unveils the intricate syntrophic dynamics underlying reductive dechlorination.

Graphical Abstract
Migratory-derived resources induce elongated food chains through middle-up food web effects
Authors: Moccetti Coralie , Sperlich Nicola , Saboret Grégoire , ten Brink Hanna , Brodersen Jakob
Published: August 2024

Background

Seasonal movements of animals often result in the transfer of large amounts of energy and nutrients across ecosystem boundaries, which may have large consequences on local food webs through various pathways. While this is known for both terrestrial- and aquatic organisms, quantitative estimates on its effects on food web structure and identification of key pathways are scarce, due to the difficulty in obtaining replication on ecosystem level with negative control, i.e. comparable systems without migration.

Methods

In this study, we estimate the impact of Arctic charr (Salvelinus alpinus) migration on riverine ecosystem structure, by comparing multiple streams with strictly resident populations above natural migration barriers with streams below those barriers harboring partially migratory populations. We compared density estimates and size structure between above and below populations. Diet differences were examined through the analysis of stomach contents, changes in trophic position were examined by using stable isotopes. To infer growth rate of resident individuals, back-growth calculation was performed using otoliths.

Results

We find higher densities of small juveniles in partially migratory populations, where juvenile Arctic charr show initially lower growth, likely due to higher intraspecific competition. After reaching a size, where they can start feeding on eggs and smaller juveniles, which are both more frequent in partially migratory populations, growth surpasses that of resident populations. Cannibalism induced by high juvenile densities occurred almost exclusively in populations with migration and represents an altered energy pathway to the food web. The presence of large cannibalistic charr feeding on smaller ones that have a similar trophic level as charr from strictly resident populations (based on stomach content) coupled with steeper δ15N-size regression slopes illustrate the general increase of food chain length in systems with migration.

Conclusions

Our results thus suggest that the consumption of migration-derived resources may result in longer food chains through middle-up rather than bottom-up effects. Furthermore, by occupying the apex of the food chain and feeding on juvenile conspecifics, resident individuals experience reduced competition with their young counterparts, which potentially balances their fitness with migratory individuals.
Morphological MRI features as prognostic indicators in brain metastases
Authors: Ocaña-Tienda Beatriz , Pérez-Beteta Julián , Ortiz de Mendivil Ana , Asenjo Beatriz , Albillo David , Pérez-Romasanta Luís A. , LLorente Manuel , Carballo Natalia , Arana Estanislao , Pérez-García Víctor M.
Published: August 2024

Background

Stereotactic radiotherapy is the preferred treatment for managing patients with fewer than five brain metastases (BMs). However, some lesions recur after irradiation. The purpose of this study was to identify patients who are at a higher risk of failure, which can help in adjusting treatments and preventing recurrence.

Methods

In this retrospective multicenter study, we analyzed the predictive significance of a set of interpretable morphological features derived from contrast-enhanced (CE) T1-weighted MR images as imaging biomarkers using Kaplan–Meier analysis. The feature sets studied included the total and necrotic volumes, the surface regularity and the CE rim width. Additionally, we evaluated other nonmorphological variables and performed multivariate Cox analysis.

Results

A total of 183 lesions in 128 patients were included (median age 61 [31–95], 64 men and 64 women) treated with stereotactic radiotherapy (57% single fraction, 43% fractionated radiotherapy). None of the studied variables measured at diagnosis were found to have prognostic value. However, the total and necrotic volumes and the CE rim width measured at the first follow-up after treatment and the change in volume due to irradiation can be used as imaging biomarkers for recurrence. The optimal classification was achieved by combining the changes in tumor volume before and after treatment with the presence or absence of necrosis (p <  < 0.001).

Conclusion

This study demonstrated the prognostic significance of interpretable morphological features extracted from routine clinical MR images following irradiation in brain metastases, offering valuable insights for personalized treatment strategies.
Cortical dynamics of perception as trains of coherent gamma oscillations, with the pulvinar as central coordinator
Authors: Farineau J. , Lestienne R.
Published: August 2024

Synchronization of spikes carried by the visual streams is strategic for the proper binding of cortical assemblies, hence for the perception of visual objects as coherent units. Perception of a complex visual scene involves multiple trains of gamma oscillations, coexisting at each stage in visual and associative cortex. Here, we analyze how this synchrony is managed, so that the perception of each visual object can emerge despite this complex interweaving of cortical activations. After a brief review of structural and temporal facts, we analyze the interactions which make the oscillations coherent for the visual elements related to the same object. We continue with the propagation of these gamma oscillations within the sensory chain. The dominant role of the pulvinar and associated reticular thalamic nucleus as cortical coordinator is the common thread running through this step-by-step description. Synchronization mechanisms are analyzed in the context of visual perception, although the present considerations are not limited to this sense. A simple experiment is described, with the aim of assessing the validity of the elements developed here. A first set of results is provided, together with a proposed method to go further in this investigation.
Pricing multi-asset options with tempered stable distributions
Authors: Xia Yunfei , Grabchak Michael
Published: August 2024

We derive methods for risk-neutral pricing of multi-asset options, when log-returns jointly follow a multivariate tempered stable distribution. These lead to processes that are more realistic than the better known Brownian motion and stable processes. Further, we introduce the diagonal tempered stable model, which is parsimonious but allows for rich dependence between assets. Here, the number of parameters only grows linearly as the dimension increases, which makes it tractable in higher dimensions and avoids the so-called “curse of dimensionality.” As an illustration, we apply the model to price multi-asset options in two, three, and four dimensions. Detailed goodness-of-fit methods show that our model fits the data very well.
Delayed recovery of consciousness from anesthesia due to exacerbation of hydrocephalus caused by a ventriculoperitoneal shunt malfunction during general anesthesia in the prone position: a case report
Authors: Miyamoto Yosuke , Kawasaki Takashi , Nakamura Shingo , Hirata Naoyuki
Published: August 2024

Background

Dysfunction of ventriculoperitoneal (VP) shunts can lead to decreased levels of consciousness. We report a case of delayed emergence from anesthesia due to the malfunction of a VP shunt during neurosurgery in the prone position.

Case presentation

A 75-year-old male with a history of VP shunt for a fourth ventricle obstruction underwent cerebral vascular anastomosis in the prone position. His preoperative level of consciousness was clear. The surgery under general anesthesia was completed without any particular issues. After discontinuation of anesthesia, the patient did not awaken for over an hour. Postoperative CT revealed exacerbated hydrocephalus, likely from VP shunt occlusion. After pumping the reservoir of the VP shunt, the patient regained consciousness. He was extubated and discharged from ICU on the second postoperative day with no neurological issues.

Conclusion

For surgical patients with a VP shunt, anesthesia management must consider the risk of shunt malfunction due to patient positioning.
Psychological assessment in school contexts: ethical issues and practical guidelines
Authors: Cadime Irene , Mendes Sofia A.
Published: August 2024

Background

Psychological assessment in school settings involves a range of complexities and ethical dilemmas that practitioners must navigate carefully. This paper provides a comprehensive review of common issues faced by school psychologists during assessments, discussing best practices and ethical guidelines based on codes from various professional organizations.

Methods

We examine the entire assessment process, from pre-assessment considerations like informed consent and instrument selection to post-assessment practices involving results communication and confidentiality. Key ethical concerns addressed include fairness in assessment, cultural and linguistic appropriateness of testing materials, and issues surrounding informed consent.

Results

Specific challenges discussed include selecting appropriate assessment instruments that reflect the diverse needs and backgrounds of students, ensuring fairness and removing bias in testing, and effectively communicating results to various stakeholders while maintaining confidentiality. We emphasize the importance of multi-source, multi-method assessment approaches and the critical role of ongoing professional development in ethical practice.

Conclusion

By adhering to established ethical standards and best practices, school psychologists can effectively support the educational and developmental needs of students. This paper outlines actionable recommendations and ethical considerations to help practitioners enhance the accuracy, fairness, and impact of their assessments in educational settings.
Pathogenicity of native strains of Bacillus thuringiensis, Beauveria bassiana and Metarhizium rileyi as entomopathogens against the polyphagous borer, Conogethes punctiferalis (Guenée) (Crambidae: Lepidoptera)
Authors: Duraimurugan P. , Bharathi E. , Dharavath Neethu Roy , Selvam Hariharan
Published: August 2024

Background

The shoot and fruit borer, Conogethes punctiferalis (Guenée) (Crambidae: Lepidoptera), is a significant pest causing substantial economic losses across various crops. The need for alternative control methods has prompted the exploration of biological control using entomopathogenic fungi and bacteria. In this study, the pathogenicity of Beauveria bassiana, Metarhizium (Nomuraea) rileyi and Bacillus thuringiensis (Bt) against C. punctiferalis larvae and pupae was assessed through laboratory bioassays.

Results

Various concentrations of B. bassiana and M. rileyi spores, i.e. 1 × 102, 1 × 104, 1 × 106, 1 × 107 and 1 × 108 spores/ml, were tested alongside controls. Additionally, five strains of Bt (IIOR Bt-145, Bt-154, Bt-171, Bt-172 and Bt-127) were evaluated at concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5 mg/ml. The LC50 values for B. bassiana and M. rileyi were 7.9 × 105 spores/ml and 8.7 × 104 spores/ml, respectively, after 4 and 6 days of post-treatment using the spray method. In the larval dip method, the LC50 values were 4.8 × 103 spores/ml for B. bassiana and 2.0 × 104 spores/ml for M. rileyi after 5 and 6 days of treatment, respectively. For pupae, the LC50 values were 1.2 × 106 spores/ml for B. bassiana and 4.3 × 104 spores/ml for M. rileyi after 4 and 7 days of treatment, respectively. Similarly, the five strains of Bt were effective against C. punctiferalis. However, Bt-154 demonstrated the highest efficacy, with LC50 values of 0.66 mg/ml in the spray method and 0.79 mg/ml in the larval dip method after 5 days of post-treatment.

Conclusion

The potential of entomopathogenic isolates as biocontrol agents against C. punctiferalis provided a promising alternative to synthetic insecticides in pest management. The efficacy of B. bassiana, M. rileyi and Bt strains suggests their suitability for integrated pest management strategies, potentially reducing reliance on chemical pesticides and minimizing the environmental impacts. Further field studies are warranted to validate the efficacy and practicality of these biocontrol agents in real-world agricultural settings.
Disorders of organic acid metabolism and epilepsy
Authors: Shi Yuqing , Wei Zihan , Feng Yan , Gan Yajing , Li Guoyan , Deng Yanchun
Published: August 2024

Epilepsy can be caused by a variety of causes, such as inborn errors of metabolism, organic acid disorders are the most significant type of metabolic disorders that cause seizures. The clinical manifestations of these diseases are generally nonspecific, and the types of seizures are different. Screening for multisystem clinical symptoms and identifying the underlying etiology are crucial for early treatment of epileptic seizures. This article provides a comprehensive summary of the pathogenesis, clinical features, diagnosis and treatment of epilepsy associated with organic acid metabolism disorders. Furthermore, relevant literature has also been reviewed to assist clinicians in the diagnosis of cases characterized by the coexistence of multisystemic symptoms and epileptic manifestations.
Stability-indicating liquid chromatographic method development and validation for quantification of trifarotene in pure and topical drug product
Authors: Usman Muhammad , Shafique Muhammad Bilal
Published: August 2024

Background

Trifarotene is effective for treating acne and other skin issues. To ensure its quality and meet regulatory standards, a reverse phase liquid chromatography (RP-LC) stability-indicating method was developed and validated for its quantification in pure and topical dosage forms. An isocratic elution chromatographic method was employed, using an octadecylsilyl silica gel-packed column (150 mm × 4.6 mm, 3 µm particle size). The mobile phase was a mixture of phosphate buffer and acetonitrile (40:60 v/v). Chromatographic conditions included a flow rate of 0.5 mL/min, column temperature of 40 °C, detection at 265 nm, and injection volume of 20 µL.

Results

The developed analytical method reports the retention time of trifarotene 11.2 min, higher theoretical plate count, asymmetric peak, and good resolution between the peaks of trifarotene, phenoxyethanol, and environmentally generated impurities.

Conclusion

The analytical method has been found to be linear, accurate, robust, specific, and selective for impurities produced during forced degradation studies. The proposed analytical method can be utilized for routine pharmaceutical analysis of trifarotene to judge its quality and safety.
Circadian Alignment, Cardiometabolic Disease, and Sex-Specific Differences in Adults With Overweight/Obesity
Authors: Shafer Brooke M , Kogan Sophia A , Rice Sean P M , Shea Steven A , Olson Ryan , McHill Andrew W
Published: August 2024

Context

Circadian disruption promotes weight gain and poor health. The extent to which sex plays a role in the relationship between the circadian timing of behaviors and health outcomes in individuals with overweight/obesity is unclear.

Objective

We investigated the sex-specific associations between circadian alignment and cardiometabolic health markers in females and males with overweight/obesity.

Methods

Thirty volunteers with overweight/obesity (15 female; body mass index ≥25.1 kg/m2) underwent an evening in-laboratory assessment for dim-light melatonin onset (DLMO), body composition via dual energy x-ray absorptiometry, and a fasted blood sample. Circadian alignment was determined as the time difference between DLMO and average sleep onset over 7 days (phase angle), with participants categorized into narrow/wide phase angle groups based on median phase angle split. Due to known differences in metabolic markers between sexes, participants were subdivided based on sex into narrow and wide phase angle groups.

Results

Males in the narrow phase angle group had higher android/gynoid body fat distribution, triglycerides, and metabolic syndrome risk scores, while females had higher overall body fat percentage, glucose, and resting heart rates (all P < .04). Furthermore, a narrower phase angle in males was negatively associated with android/gynoid body fat (r = −0.53, P = .04) and negatively associated with body fat (r = −0.62, P = .01) and heart rate (r = −0.73, P < .01) in females.

Conclusion

Circadian disruption may not only promote a trajectory of weight gain but could also contribute to negative health consequences in a sex-dependent manner in those already with overweight/obesity. These data may have implications for clinical utility in sex-specific sleep and circadian interventions for adults with overweight/obesity.
Fantasies of Nina Simone
Authors: Stein Jordan Alexander
Published: August 2024

Since her death in 2003, Nina Simone has been the subject of an astonishing number of rereleased, remastered, and remixed albums and compilations as well as biographies, films, viral memes, samples, and soundtracks. In Fantasies of Nina Simone, Jordan Alexander Stein uses an archive of Simone’s performances, images, and writings to examine the space between our collective and individual fantasies about Simone the performer, civil rights activist, and icon, and her own fantasies about herself. Stein outlines how Simone gave voice to personal fantasies through releasing dozens of covers of her white male contemporaries. With her covers of George Harrison, the Bee Gees, Bob Dylan, and others, Simone explored and claimed the power and perspective that come with race and gender privilege. Looking at examples from Simone’s four-decade genre-bending career—from songbook standards, jazz, and pop to folk, junkanoo, and reggae—and at her work’s many uptakes and afterlives, Stein mobilizes the psychoanalytic concept of fantasy to build a black feminist history with and for this multifaceted performing artist.