bluuebunny/crossref_metadata_2025 huggingface dataset:
Survival Status of Esophageal Cancer Patients and its Determinants in Ethiopia: A Facility Based Retrospective Cohort Study
Authors: Hassen Hamid Yimam , Teka Mohammed Ahmed , Addisse Adamu
Published: February 2021

Background

Globally, the incidence and mortality due to esophageal cancer are increasing, particularly in low- and middle-income countries. Cancer of the esophagus is the eighth in incidence and seventh in cancer mortality in Ethiopia. A few studies have shown an increasing burden, however, little is known about the survival pattern and its determinants among esophageal cancer patients in Ethiopia. Therefore, we assessed the survival pattern and its determinants among esophageal cancer patients.

Methods

We conducted a retrospective cohort study among 349 esophageal cancer patients who were diagnosed at or referred to Tikur Anbessa Specialized Hospital, Ethiopia from January 2010 to May 2017. Using an abstraction form, nurses who were working at the oncology department extracted the data from patient charts. To estimate and compare the probability of survival among covariate categories, we performed a Kaplan–Meier survival analysis with the log-rank test. To identify the prognostic determinants of survival, we performed a multivariable Cox proportional regression analysis.

Results

The median follow-up time was 32 months with interquartile range of 15 to 42. Overall, the median survival time after diagnosis with esophageal cancer was 4 months with one-, two- and three-year survival of 14.4, 6.3, and 2.4% respectively. In the multivariable Cox proportional hazards model, receiving chemotherapy [Adjusted Hazard Ratio (AHR)=0.36, 95%CI: 0.27–0.49], radiotherapy [AHR=0.38, 95%CI: 0.23–0.63] and surgery [AHR=0.70, 95%CI: 0.54–0.89] were statistically significant.

Conclusions

In Ethiopia, esophageal cancer patients have a very low one-, two- and three-year survival. Despite a very low overall survival, patients who received either chemotherapy, radiotherapy or surgery showed a better survival compared with those who did not receive any treatment. Hence, it is essential to improve the survival of patients with esophageal cancer through early detection and timely initiation of the available treatment options.
Stillbirths, Neonatal Morbidity, and Mortality in Health-Facility Deliveries in Urban Gambia
Authors: Camara Bully , Oluwalana Claire , Miyahara Reiko , Lush Alyson , Kampmann Beate , Manneh Kebba , Okomo Uduak , D'Alessandro Umberto , Roca Anna
Published: February 2021

Background: The Gambia Demographic and Health Survey 2013 data showed that up to 63% of deliveries in the country occur in health facilities. Despite such a high rate, there are few facility-based studies on delivery outcomes in the country. This analysis ancillary to a randomized control trial describes occurrence of poor pregnancy outcomes in a cohort of women and their infants delivering in a government health facility in urban Gambia.

Methods: Using clinical information obtained during the trial, we calculated rates of poor pregnancy outcomes including stillbirths, hospitalization and neonatal deaths. Logistic regression was used to calculate odds ratio (OR) and 95% confidence interval (CI) in the risk factors analysis.

Results: Between April 2013 and 2014, 829 mothers delivered 843 babies, including 13 stillbirths [15.4 (7.1–23.8)] per 1,000 births. Among 830 live born infants, 7.6% (n = 63) required hospitalization during the 8-week follow-up period. Most of these hospitalizations (74.6%) occurred during the early neonatal period (<7 days of life). Severe clinical infections (i.e., sepsis, meningitis and pneumonia) (n = 27) were the most common diagnoses, followed by birth asphyxia (n = 13), major congenital malformations (n = 10), jaundice (n = 6) and low birth weight (n = 5). There were sixteen neonatal deaths, most of which also occurred during the early neonatal period. Overall, neonatal mortality rate (NMR) and perinatal mortality rate (PMR) were 19.3 (CI: 9.9–28.7) per 1,000 live births and 26.1 (CI: 15.3–36.9) per 1,000 total births, respectively. Severe clinical infections and birth asphyxia accounted for 37 and 31% of neonatal deaths, respectively. The risk of hospitalization was higher among neonates with severe congenital malformations, low birth weight, twin deliveries, and those born by cesarean section. Risk of mortality was higher among neonates with severe congenital malformations and twin deliveries.

Conclusion: Neonatal hospitalization and deaths in our cohort were high. Although vertical interventions may reduce specific causes of morbidity and mortality, data indicate the need for a holistic approach to significantly improve the rates of poor pregnancy outcomes. Critically, a focus on decreasing the high rate of stillbirths is warranted.

Clinical Trial Registration:ClinicalTrials.gov Identifier: NCT01800942.
Vanillic Acid Reduces Pain-Related Behavior in Knee Osteoarthritis Rats Through the Inhibition of NLRP3 Inflammasome-Related Synovitis
Authors: Ma Zhenyuan , Huang Zhengquan , Zhang Li , Li Xiaochen , Xu Bo , Xiao Yancheng , Shi Xiaoqing , Zhang Haosheng , Liao Taiyang , Wang Peimin
Published: February 2021

Objectives: Synovitis plays an important role in knee osteoarthritis (KOA) pain. The activation of the NOD-like receptor protein 3 (NLRP3) inflammasome in fibroblast-like synoviocytes (FLSs) promotes KOA development. In this study, we aimed to investigate whether vanillic acid (VA), a monomer derived from Chinese herbal medicines, could target NLRP3 inflammasome-related synovitis to reduce pain.

Methods: Rats in the KOA and KOA + VA groups were injected with monosodium iodoacetate (MIA) in the knee to induce KOA. From day 14, the KOA + VA group was given VA at 30 mg/kg every day via gastric intubation. FLSs were collected from the synovial tissues. We examined both the protein and gene expression of caspase-1, apoptosis-associated speck-like protein with a caspase recruitment domain (ASC), NLRP3, components of the NLRP3 inflammasome, and interleukin-1β (IL-1β) and IL-18 in vivo and in vitro.

Results: The upregulation of caspase-1, ASC, and NLRP3 in the KOA model were reduced by VA. VA also lowered the level of IL-1β and IL-18 in the KOA model. In addition, VA relieved pain-related behavior of KOA model rats and downregulated the pain mediators CGRP, NGF, and TrkA in FLSs. Interestingly, we also observed reduced synovial fibrosis in the animal experiments.

Conclusion: Our research showed that VA reduces synovitis and pain-related behaviors in a rat model of KOA, which provides the basis for further investigations into the potential therapeutic impact of VA in KOA.
Identification and Functional Analysis of Two Purple Acid Phosphatases AtPAP17 and AtPAP26 Involved in Salt Tolerance in Arabidopsis thaliana Plant
Authors: Abbasi-Vineh Mohammad Ali , Sabet Mohammad Sadegh , Karimzadeh Ghasem
Published: February 2021

Tolerance to salinity is a complex genetic trait including numerous physiological processes, such as metabolic pathways and gene networks; thereby, identification of genes indirectly affecting, as well as those directly influencing, is of utmost importance. In this study, we identified and elucidated the functional characterization of AtPAP17 and AtPAP26 genes, as two novel purple acid phosphatases associated with high-salt tolerance in NaCl-stressed conditions. Here, the overexpression of both genes enhanced the expression level of AtSOS1, AtSOS2, AtSOS3, AtHKT1, AtVPV1, and AtNHX1 genes, involving in the K+/Na+ homeostasis pathway. The improved expression of the genes led to facilitating intracellular Na+ homeostasis and decreasing the ion-specific damages occurred in overexpressed genotypes (OEs). An increase in potassium content and K+/Na+ ratio was observed in OE17 and OE26 genotypes as well; however, lower content of sodium accumulated in these plants at 150 mM NaCl. The overexpression of these two genes resulted in the upregulation of the activity of the catalase, guaiacol peroxidase, and ascorbate peroxidase. Consequently, the overexpressed plants showed the lower levels of hydrogen peroxide where the lowest amount of lipid peroxidation occurred in these lines. Besides the oxidation resistance, the boost of the osmotic regulation through the increased proline and glycine-betaine coupled with a higher content of pigments and carbohydrates resulted in significantly enhancing biomass production and yield in the OEs under 150 mM NaCl. High-salt stress was also responsible for a sharp induction on the expression of both PAP17 and PAP26 genes. Our results support the hypothesis that these two phosphatases are involved in plant responses to salt stress by APase activity and/or non-APase activity thereof. The overexpression of PAP17 and PAP26 could result in increasing the intracellular APase activity in both OEs, which exhibited significant increases in the total phosphate and free Pi content compared to the wild-type plants. Opposite results witnessed in mutant genotypes (Mu17, Mu26, and DM), associating with the loss of AtPAP17 and AtPAP26 functions, clearly confirmed the role of these two genes in salt tolerance. Hence, these genes can be used as candidate genes in molecular breeding approaches to improve the salinity tolerance of crop plants.
Spatio-Temporal Dynamics of the Patterning of Arabidopsis Flower Meristem
Authors: Díaz José , Álvarez-Buylla Elena R.
Published: February 2021

The qualitative model presented in this work recovers the onset of the four fields that correspond to those of each floral organ whorl ofArabidopsisflower, suggesting a mechanism for the generation of the positional information required for the differential expression of the A, B, and C identity genes according to the ABC model for organ determination during early stages of flower development. Our model integrates a previous model for the emergence of WUS pattern in the floral meristem, and shows that this pre-pattern is a necessary but not sufficient condition for the posterior information of the four fields predicted by the ABC model. Furthermore, our model predicts that LFY diffusion along the L1 layer of cells is not a necessary condition for the patterning of the floral meristem.
The CLV3 Homolog in Setaria viridis Selectively Controls Inflorescence Meristem Size
Authors: Zhu Chuanmei , Liu Lei , Crowell Olivia , Zhao Hui , Brutnell Thomas P. , Jackson David , Kellogg Elizabeth A.
Published: February 2021

The CLAVATA pathway controls meristem size during inflorescence development in both eudicots and grasses, and is initiated by peptide ligands encoded byCLV3/ESR-related (CLE) genes. While CLV3 controls all shoot meristems inArabidopsis, evidence from cereal grasses indicates that different meristem types are regulated by different CLE peptides. The rice peptide FON2 primarily controls the size of the floral meristem, whereas the orthologous peptides CLE7 and CLE14 in maize have their most dramatic effects on inflorescence and branch meristems, hinting at diversification among CLE responses in the grasses.Setaria viridisis more closely related to maize than to rice, so can be used to test whether the maize CLE network can be generalized to all members of subfamily Panicoideae. We used CRISPR-Cas9 inS. viridisto knock out theSvFON2gene, the closest homolog toCLV3andFON2.Svfon2mutants developed larger inflorescence meristems, as in maize, but had normal floral meristems, unlikeOsfon2, suggesting a panicoid-specific CLE network. Vegetative traits such as plant height, tiller number and leaf number were not significantly different between mutant and wild type plants, but time to heading was shorter in the mutants.In situhybridization showed strong expression ofSvfon2in the inflorescence and branch meristems, consistent with the mutant phenotype. Using bioinformatic analysis, we predicted the co-expression network ofSvFON2and its signaling components, which included genes known to control inflorescence architecture in maize as well as genes of unknown function. The similarity between SvFON2 function in Setaria and maize suggests that its developmental specialization in inflorescence meristem control may be shared among panicoid grasses.
An Update on the Role of the Actin Cytoskeleton in Plasmodesmata: A Focus on Formins
Authors: Diao Min , Huang Shanjin
Published: February 2021

Cell-to-cell communication in plants is mediated by plasmodesmata (PD) whose permeability is tightly regulated during plant growth and development. The actin cytoskeleton has been implicated in regulating the permeability of PD, but the underlying mechanism remains largely unknown. Recent characterization of PD-localized formin proteins has shed light on the role and mechanism of action of actin in regulating PD-mediated intercellular trafficking. In this mini-review article, we will describe the progress in this area.
Sleep and Safety Improve Physicians’ Psychological Functioning at Work During Covid-19 Epidemic
Authors: Zupancic Nina , Bucik Valentin , Ihan Alojz , Dolenc-Groselj Leja
Published: February 2021

Purpose

The COVID-19 pandemic caused a massive healthcare crisis. To investigate what makes healthcare system resilient and physicians better at coping during a crisis situation, our study investigated the role risk exposure, such as working at COVID-19 entry points, sleep, and perceived work safety played in reducing negative psychological functioning at work, as well as their effects on adverse and potentially fatal incidences of compromised safety and medical errors.

Methods

Our study included a representative sample of 1,189 physicians, from all 12 Slovenian regions and all medical occupations, as registered by the Medical Chamber of Slovenia. For the purposes of this study, a Questionnaire of Sleep and Psychological Functioning at Work was developed in the form of an online retrospective self-report. Additionally, our study included items assessing physicians perceived work safety and frequency of negative outcomes (compromised safety and medical errors) during the first month of the Covid-19 epidemic.

Results

Physicians working at COVID-19 entry points were more likely to experience night awakening, slept less than 5 h per night, experience nightmares, and had lower levels of psychological functioning in comparison to other physicians. Both hypothesized models showed adequate fit. A higher score on the sleep scale (sleep quantity, sleep quality, and shorter sleep latency) has been shown to predict lower levels of negative psychological functioning at work and, indirectly, reduced incidences of compromised safety and medical errors. Contrary to our expectations, no significant direct effect of sleep on compromised safety and medical errors was found. When perceived work safety was added into the model, the model showed improved fit, with perceived work safety predicting better sleep, less negative psychological functioning at work, and less compromised safety.

Conclusion

Sleep and safety both play an important role in reducing negative psychological functioning at work and, by doing so, decreasing the negative and potentially fatal incidents during the pandemic, such as compromised safety and medical errors. Further, research is needed to see how medical guidelines can be updated to ensure physicians sleep and that their safety is protected.
Determining the Number of Attributes in Cognitive Diagnosis Modeling
Authors: Nájera Pablo , Abad Francisco José , Sorrel Miguel A.
Published: February 2021

Cognitive diagnosis models (CDMs) allow classifying respondents into a set of discrete attribute profiles. The internal structure of the test is determined in a Q-matrix, whose correct specification is necessary to achieve an accurate attribute profile classification. Several empirical Q-matrix estimation and validation methods have been proposed with the aim of providing well-specified Q-matrices. However, these methods require the number of attributes to be set in advance. No systematic studies about CDMs dimensionality assessment have been conducted, which contrasts with the vast existing literature for the factor analysis framework. To address this gap, the present study evaluates the performance of several dimensionality assessment methods from the factor analysis literature in determining the number of attributes in the context of CDMs. The explored methods were parallel analysis, minimum average partial, very simple structure, DETECT, empirical Kaiser criterion, exploratory graph analysis, and a machine learning factor forest model. Additionally, a model comparison approach was considered, which consists in comparing the model-fit of empirically estimated Q-matrices. The performance of these methods was assessed by means of a comprehensive simulation study that included different generating number of attributes, item qualities, sample sizes, ratios of the number of items to attribute, correlations among the attributes, attributes thresholds, and generating CDM. Results showed that parallel analysis (with Pearson correlations and mean eigenvalue criterion), factor forest model, and model comparison (with AIC) are suitable alternatives to determine the number of attributes in CDM applications, with an overall percentage of correct estimates above 76% of the conditions. The accuracy increased to 97% when these three methods agreed on the number of attributes. In short, the present study supports the use of three methods in assessing the dimensionality of CDMs. This will allow to test the assumption of correct dimensionality present in the Q-matrix estimation and validation methods, as well as to gather evidence of validity to support the use of the scores obtained with these models. The findings of this study are illustrated using real data from an intelligence test to provide guidelines for assessing the dimensionality of CDM data in applied settings.
Depressive and Anxiety Symptoms Among People Under Quarantine During the COVID-19 Epidemic in China: A Cross-Sectional Study
Authors: Yan Tong , Zhizhong Wang , Jianzhong Zheng , Yubo Ying , Jie Liu , Junjun Zhang , Guangtian Liu
Published: February 2021

Background: During the coronavirus disease 2019 (COVID-19) pandemic, quarantine as an effective public health measure has been widely used in China and elsewhere to slow down the spread, while high-risk psychological response populations remain under-reported.

Objective: The aim of the study is to investigate the depressive and anxiety symptoms among the high-risk individuals quarantined during the COVID-19 pandemic in China.

Methods: An online survey was conducted from February 29 to April 10, 2020, among individuals quarantined for at least 2 weeks due to the high-risk exposure. Chinese versions of the nine-item Patient Health Questionnaire (PHQ-9) with a seven-item Generalized Anxiety Disorder (GAD-7) were applied to assess depressive and anxiety symptoms, respectively. Compliance with quarantine and knowledge of COVID-19 was also assessed. An unconditional logistic regression model was performed to identify the correlators.

Results: Of the 1,260 participants completing the full survey, 14.0% (95% CI: 12.2–16.1%), 7.1% (95% CI: 5.9–8.7%), and 6.3% (95% CI: 5.1–7.8%) had at least moderate symptoms of depression, anxiety, and a combination of depression and anxiety (CDA), respectively; 14.8% (95% CI: 13.0–16.9%) had at least one condition. Multivariate analysis showed that participants with an undergraduate or above degree were more likely to report depressive (OR = 2.98, 95% CI: 1.56–5.72) and anxiety symptoms (OR = 2.95, 95% CI: 1.14–7.63) than those with middle school education. Those who were unemployed (OR = 0.37, 95% CI: 0.21–0.65 for depression; OR = 0.31, 95% CI: 0.14–0.73 for anxiety), students (OR = 0.14, 95% CI: 0.04–0.48 for depression; OR = 0.11, 95% CI: 0.01–0.85 for anxiety), and more knowledgeable of COVID-19 (OR = 0.84, 95% CI: 0.73–0.96 for depression, OR = 0.82, 95% CI: 0.68–0.98 for anxiety) were less likely to report depressive and anxiety symptoms. Higher quarantine compliance correlated with lower risks of depressive (OR = 0.94, 95% CI: 0.91–0.96) and anxiety symptoms (OR = 0.95, 95% CI: 0.91–0.98).

Conclusion: Individuals under quarantine during the COVID-19 pandemic suffered prevalent depressive and anxiety symptoms. Consequently, comprehensive interventional measures, including knowledge dissemination, timely virus tests, and strengthened communication, may minimize quarantine's adverse effects.
Risk Perception and PTSD Symptoms of Medical Staff Combating Against COVID-19: A PLS Structural Equation Model
Authors: Yin Qianlan , Chen Aibin , Song Xiangrui , Deng Guanghui , Dong Wei
Published: February 2021

Medical staff were battling against coronavirus disease 2019 (COVID-19) at the expense of their physical and mental health, particularly at risk for posttraumatic stress disorder (PTSD). In this case, intervening PTSD of medical staff and preparing them for future outbreaks are important. Previous studies showed that perceived stress was related to the development of PTSD. Hence, in this study, the association between risk perception of medical staff and PTSD symptoms in COVID-19 and the potential links were explored. Three hundred four medical staff's exposure to COVID-19 patients, risk perception for working during COVID-19, PTSD symptoms, anxiety, and sleep quality were measured. Mediation analysis tested the indirect effects of anxiety and sleep quality on the relationship between risk perceptions and PTSD symptoms; 27.6% of participants were deemed as having probable PTSD diagnosis. Mediation analysis showed a significant chain-mediating effect of anxiety and sleep quality on the relationships between risk perceptions and PTSD symptoms; higher risk perceptions were related to increased anxiety, worsened sleep quality, and severe PTSD symptoms. Conclusively, medical staff have a high prevalence of PTSD symptoms after 3 months of COVID-19. Their PTSD symptoms were associated with the perceived risk level through the potential links with anxiety and sleep quality. Therefore, risk perception could be critical for our medical staff's responses to public health emergencies. It could be plausible to intervene in the perceived stress to alleviate aroused anxiety and improve sleep quality and thereby deter the development of PTSD.
The Efficacy, Safety, and Tolerability of Probiotics on Depression: Clinical Results From an Open-Label Pilot Study
Authors: Wallace Caroline J. K. , Milev Roumen V.
Published: February 2021

Background: A growing body of research has shown that consumption of probiotics can improve symptoms associated with mood and anxiety disorders through activity of the gut-brain axis. However, the effects of probiotics have yet to be tested in a clinical sample of treatment-naïve patients diagnosed with Major Depressive Disorder (MDD). The aim of this 8-week, open-label pilot study is to examine changes in depressive symptoms before and after the introduction of a probiotic supplement in 10 treatment-naïve MDD patients and to provide data on the feasibility of conducting a larger double-blind, randomized, placebo-controlled trial in the same patient population. Here we report on the clinical outcome measures of the study.

Methods: Participants recruited from the community in Kingston, Ontario, Canada consumed a probiotic supplement containing Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 (CEREBIOME®) at a dose of 3 × 109 CFU once per day for 8 weeks. Clinical symptoms of depression were measured using a validated battery of clinical scales and self-report questionnaires (CAN-BIND protocol). Data was collected at baseline, week 4, and week 8.

Results: Significant improvements in affective clinical symptoms were observed at week 4 and were sustained at week 8. Significant improvements in subjective sleep quality were observed by week 8. No side effects or adverse effects associated with the probiotic supplement were observed.

Conclusions: The findings from this study support the existing evidence in this emerging field for probiotics having a role in alleviating symptoms of depression in treatment-naïve, moderately depressed patients and indicate that the probiotic supplement is safe and well-tolerated in this population. However, further comprehensive studies are required to draw conclusions.
In vitro Assessment of the DNA Damage Response in Dental Mesenchymal Stromal Cells Following Low Dose X-ray Exposure
Authors: Belmans Niels , Gilles Liese , Welkenhuysen Jonas , Vermeesen Randy , Baselet Bjorn , Salmon Benjamin , Baatout Sarah , Jacobs Reinhilde , Lucas Stéphane , Lambrichts Ivo , Moreels Marjan
Published: February 2021

Stem cells contained within the dental mesenchymal stromal cell (MSC) population are crucial for tissue homeostasis. Assuring their genomic stability is therefore essential. Exposure of stem cells to ionizing radiation (IR) is potentially detrimental for normal tissue homeostasis. Although it has been established that exposure to high doses of ionizing radiation (IR) has severe adverse effects on MSCs, knowledge about the impact of low doses of IR is lacking. Here we investigated the effect of low doses of X-irradiation with medical imaging beam settings (<0.1 Gray; 900 mGray per hour),in vitro, on pediatric dental mesenchymal stromal cells containing dental pulp stem cells from deciduous teeth, dental follicle progenitor cells and stem cells from the apical papilla. DNA double strand break (DSB) formation and repair kinetics were monitored by immunocytochemistry of γH2AX and 53BP1 as well as cell cycle progression by flow cytometry and cellular senescence by senescence-associated β-galactosidase assay and ELISA. Increased DNA DSB repair foci, after exposure to low doses of X-rays, were measured as early as 30 min post-irradiation. The number of DSBs returned to baseline levels 24 h after irradiation. Cell cycle analysis revealed marginal effects of IR on cell cycle progression, although a slight G2/M phase arrest was seen in dental pulp stromal cells from deciduous teeth 72 h after irradiation. Despite this cell cycle arrest, no radiation-induced senescence was observed. In conclusion, low X-ray IR doses (< 0.1 Gray; 900 mGray per hour), were able to induce significant increases in the number of DNA DSBs repair foci, but cell cycle progression seems to be minimally affected. This highlights the need for more detailed and extensive studies on the effects of exposure to low IR doses on different mesenchymal stromal cells.
Perceived Neighborhood Crime Safety Moderates the Association Between Racial Discrimination Stress and Chronic Health Conditions Among Hispanic/Latino Adults
Authors: Budd Elizabeth L. , Giuliani Nicole R. , Kelly Nichole R.
Published: February 2021

Background:Little is known about the link between perceived neighborhood walkability and prevalence of chronic disease. Even less is known regarding this association among Hispanic/Latino adults, despite exhibiting high rates of chronic diseases. Stress due to racial discrimination is a harmful social determinant of health in Hispanics/Latinos. Having both low perceived neighborhood walkability and high racial discrimination stress may exacerbate the chronic disease status of Hispanics/Latinos. Among a U.S. national sample of Hispanic/Latino adults, this cross-sectional study aims to examine (1) the associations among overall perceived neighborhood walkability, racial discrimination stress, and having a chronic health condition; and (2) whether overall perceived neighborhood walkability moderates the hypothesized association between racial discrimination stress and having a chronic health condition.

Methods:In January 2018, 798 Hispanic/Latino adults (Mage = 39.7 years, SD = 15.1; 58.6% female; 70.0% U.S. born; 52.0% Mexican/Mexican American) responded to a survey via Qualtrics Panels. Surveys included the Neighborhood Environment Walkability Scale-Abbreviated, Hispanic Stress Inventory-2, and self-reported presence/absence of chronic health conditions (e.g., hypertension, heart disease). A logistic regression was conducted testing for the moderation of the main effect of racial discrimination stress on the presence of a chronic health condition by overall perceived neighborhood walkability.

Results:After controlling for age, body mass index, and income, racial discrimination stress was inversely associated with overall perceived neighborhood walkability (b= −0.18,p< 0.001) and positively associated with having a chronic health condition (OR = 1.02; 95% CI [1.00, 1.03]). While overall perceived neighborhood walkability was not associated with having a chronic health condition, perceived crime safety was inversely associated with having a chronic health condition (OR = 0.94; 95% CI [0.89, 0.99]). Perceived crime safety moderated the positive association between discrimination stress and having a chronic health condition, such that the association was only significant among those who perceived their neighborhood to be less safe (β = −0.004, 95% CI [−0.01, −0.00]).

Conclusions:Overall perceived neighborhood walkability was inversely associated with racial discrimination stress, but not associated with having a chronic health condition. Perceived neighborhood crime safety, but not infrastructure or aesthetics, matters when it comes to the link between racial discrimination stress and having a chronic health condition among Hispanics/Latinos.
Attitudes and Perceptions Toward Healthcare Technology Adoption Among Older Adults in Singapore: A Qualitative Study
Authors: Low Sarah T. H. , Sakhardande P. Govind , Lai Yi Feng , Long Andrew D. S. , Kaur-Gill Satveer
Published: February 2021

Smart Nation is a key initiative of Singapore to move toward digitalization of its industries including healthcare. The complex negotiations of aging amid Smart Nation are addressed in this paper, where we study the challenges faced to adapt the elderly for the digital revolution while ensuring dignified aging. While the healthcare industry accelerates its study and use of health technologies to improve diagnostics, treatment, and the quality of life of those in the aging category, the elderly socially construct these technological insertions that challenge the dominant understandings of what these technologies can do for their health outcomes. The study reveals re-constructions of these technological insertions through the voice of the elderly in their negotiations with health technologies in their everyday lives. Here, narratives reveal key themes that proliferate technology negotiation as barriers to everyday lived experiences.
Breaking the Marathon World Record With Your Father? The Superpower of Lifelong Endurance Training
Authors: Louis Julien , Bontemps Bastien , Lepers Romuald
Published: February 2021

Are you looking for a training buddy to prepare for your next marathon race? Have you ever considered that your father could be the right person? Would not it be great to cross the finish line side by side and get on the podium together? You probably think that your father is not young enough for such an effort, do not you? We believe that your father may also be capable of exceptional physical performance, despite his older age. In this article, you will see how a father and son (with a 25-year age difference) managed to break the world record for a combined father-and-son marathon in 2019.
RR Lyrae Stars as Seen by the Kepler Space Telescope
Authors: Plachy Emese , Szabó Róbert
Published: February 2021

The unprecedented photometric precision along with the quasi-continuous sampling provided by the Kepler space telescope revealed new and unpredicted phenomena that reformed and invigorated RR Lyrae star research. The discovery of period doubling and the wealth of low-amplitude modes enlightened the complexity of the pulsation behavior and guided us toward nonlinear and nonradial studies. Searching and providing theoretical explanation for these newly found phenomena became a central question, as well as understanding their connection to the oldest enigma of RR Lyrae stars, the Blazhko effect. We attempt to summarize the highest impact RR Lyrae results based on or inspired by the data of the Kepler space telescope both from the nominal and from the K2 missions. Besides the three most intriguing topics, the period doubling, the low-amplitude modes, and the Blazhko effect, we also discuss the challenges of Kepler photometry that played a crucial role in the results. The secrets of these amazing variables, uncovered by Kepler, keep the theoretical, ground-based, and space-based research inspired in the post-Kepler era, since light variation of RR Lyrae stars is still not completely understood.
Rhizosphere Engineering With Plant Growth-Promoting Microorganisms for Agriculture and Ecological Sustainability
Authors: Hakim Sughra , Naqqash Tahir , Nawaz Muhammad Shoib , Laraib Iqra , Siddique Muhammad Jawad , Zia Rabisa , Mirza Muhammad Sajjad , Imran Asma
Published: February 2021

The rhizosphere is undoubtedly the most complex microhabitat, comprised of an integrated network of plant roots, soil, and a diverse consortium of bacteria, fungi, eukaryotes, and archaea. The rhizosphere conditions have a direct impact on crop growth and yield. Nutrient-rich rhizosphere environments stimulate plant growth and yield and vice versa. Extensive cultivation exhaust most of the soils which need to be nurtured before or during the next crop. Chemical fertilizers are the major source of crop nutrients but their uncontrolled and widespread usage has posed a serious threat to the sustainability of agriculture and stability of an ecosystem. These chemicals are accumulated in the soil, drained in water, and emitted to the air where they persist for decades causing a serious threat to the overall ecosystem. Plant growth-promoting rhizobacteria (PGPR) present in the rhizosphere convert many plant-unavailable essential nutrients e.g., nitrogen, phosphorous, zinc, etc. into available forms. PGPR produces certain plant growth hormones (such as auxin, cytokinin, and gibberellin), cell lytic enzymes (chitinase, protease, hydrolases, etc.), secondary metabolites, and antibiotics, and stress alleviating compounds (e.g., 1-Aminocyclopropane-1- carboxylate deaminase), chelating agents (siderophores), and some signaling compounds (e.g., N-Acyl homoserine lactones) to interact with the beneficial or pathogenic counterparts in the rhizosphere. These multifarious activities of PGPR improve the soil structure, health, fertility, and functioning which directly or indirectly support plant growth under normal and stressed environments. Rhizosphere engineering with these PGPR has a wide-ranging application not only for crop fertilization but developing eco-friendly sustainable agriculture. Due to severe climate change effects on plants and rhizosphere biology, there is growing interest in stress-resilient PGPM and their subsequent application to induce stress (drought, salinity, and heat) tolerance mechanism in plants. This review describes the three components of rhizosphere engineering with an explicit focus on the broader perspective of PGPM that could facilitate rhizosphere engineering in selected hosts to serve as an efficient component for sustainable agriculture.
The Role of Series Cholecystectomy in High Risk Acute Cholecystitis Patients Who Underwent Gallbladder Drainage
Authors: Wang Chi-Chih , Tseng Ming-Hseng , Wu Sheng-Wen , Yang Tzu-Wei , Sung Wen-Wei , Wang Yao-Tung , Lee Hsiang-Lin , Shiu Bei-Hao , Lin Chun-Che , Tsai Ming-Chang
Published: February 2021

Background:Cholecystectomy (CCY) is the only definitive therapy for acute cholecystitis. We conducted this study to evaluate which patients may not benefit from further CCY after percutaneous transhepatic gallbladder drainage (PTGBD) has been performed in acute cholecystitis patients.

Methods:Acute cholecystitis patients with PTGBD treatment were selected from one million random samples from the National Health Insurance Research Database obtained between January 2004 and December 2010. Recurrent biliary events (RBEs), RBE-related medical costs, RBE-related mortality rate and an RBE-free survival curve were compared in patients who accepted CCY within 2 months and patients without CCY within 2 months after the index admission.

Results:Three hundred and sixty-five acute cholecystitis patients underwent PTGBD at the index admission. A total of 190 patients underwent further CCY within 2 months after the index admission. The other 175 patients did not accept further CCY within 2 months after the index admission. RBE-free survival was significantly better in the CCY within 2 months group (60 vs. 42%,p< 0.001). The RBE-free survival of the CCY within 2 months group was similar to that of the no CCY within 2 months group in patients ≥ 80 years old and patients with a Charlson Comorbidity Index (CCI) score ≥ 9.

Conclusions:We confirmed CCY after PTGBD reduced RBEs, RBE-related medical expenses, and the RBE-related mortality rate in patients with acute cholecystitis. In patients who accepted PTGBD, the RBE and survival benefits of subsequent CCY within 2 months became insignificant in patients ≥ 80 years old or with a CCI score ≥ 9.
A Perspective on the Use of Sexed Semen to Reduce the Number of Surplus Male Dairy Calves in Ireland: A Pilot Study
Authors: Balzani Agnese , Aparacida Vaz do Amaral Cintia , Hanlon Alison
Published: February 2021

The production of surplus male offspring illustrates a socioethical concern in the dairy industry. In this article, we highlight the animal health and welfare implications of production outputs for surplus dairy calves, namely veal production, dairy calf to beef production, and euthanasia. Moreover, we present a pilot study focus on exploring the perception of key industry actors within the dairy industry in Ireland regarding the use of sexed semen as a mitigation strategy to reduce the production of surplus male dairy calves. A pilot survey was completed by farmers (n = 6), veterinarians (n = 17), and dairy farm advisors (n = 11). All the veterinarians, 80% of the farmers, and 62% of the advisors believed that the use of sexed semen had a positive influence on herd welfare. All participants identified the same barriers to the implementation of sexed semen: lower conception rate, lower availability, and higher cost. The reviewed literature highlights the importance of tailored communication to support knowledge exchange between stakeholders and key industry actors such as dairy farmers, their veterinarians, and advisors. Research to understand stakeholders' perception is pivotal to address socioethical concerns such as the surplus male dairy calves.
The Association Between the Use of Oclacitinib and Antibacterial Therapy in Dogs With Allergic Dermatitis: A Retrospective Case-Control Study
Authors: Rynhoud Hester , Gibson Justine S. , Meler Erika , Soares Magalhães Ricardo J.
Published: February 2021

Background: Canine allergic dermatitis, including atopic dermatitis, often requires antibacterial therapy for concurrent infections. Oclacitinib is indicated for treatment of pruritus associated with allergic dermatitis and the clinical manifestations of atopic dermatitis in dogs aged ≥12 months.

Hypothesis/Objectives: We aimed to determine if there was a quantitative difference in antibacterial use by dogs with allergic dermatitis receiving oclacitinib vs. other anti-pruritic therapies and before vs. after oclacitinib.

Animals: In this retrospective case-control study, cases (n = 58) included dogs suffering from allergic dermatitis aged ≥12 months receiving oclacitinib and controls (n = 205) were counterpart dogs treated with other anti-pruritic therapies.

Methods: Clinical histories of dogs with allergic dermatitis were collected from a small animal university hospital. Multivariable logistic regression models were developed adjusting for underlying skin or ear conditions to determine whether cases were prescribed fewer antibacterials than controls.

Results: The odds of systemic antibacterial usage were lower in cases vs. controls [odds ratio (OR): 0.29 (95% confidence interval 0.12–0.71); P = 0.007]. The odds of amoxycillin clavulanic acid usage (12.5–25 mg/kg orally every 12 h) was lower in cases vs. controls [OR: 0.08 (0.01–0.71); P = 0.024]. Topical antibacterial drug use was reduced overall; however, only the odds of neomycin use was lower in cases vs. controls [OR: 0.3 (0.1–0.89); P = 0.029]. Cases had higher odds of experiencing improvements in allergic dermatitis categories vs. controls [OR: 7.89 (3.26–19.13); P < 0.001].

Conclusions and Clinical Importance: Our results suggest that use of oclacitinib to treat allergic dermatitis in dogs is associated with less antibacterial use than other anti-pruritic therapies.
Network Inference from Gene Expression Data with Distance Correlation and Network Topology Centrality
Authors: Liu Kuan , Liu Haiyuan , Sun Dongyan , Zhang Lei
Published: February 2021

The reconstruction of gene regulatory networks based on gene expression data can effectively uncover regulatory relationships between genes and provide a deeper understanding of biological control processes. Non-linear dependence is a common problem in the regulatory mechanisms of gene regulatory networks. Various methods based on information theory have been developed to infer networks. However, the methods have introduced many redundant regulatory relationships in the network inference process. A recent measurement method called distance correlation has, in many cases, shown strong and computationally efficient non-linear correlations. In this paper, we propose a novel regulatory network inference method called the distance-correlation and network topology centrality network (DCNTC) method. The method is based on and extends the Local Density Measurement of Network Node Centrality (LDCNET) algorithm, which has the same choice of network centrality ranking as the LDCNET algorithm, but uses a simpler and more efficient distance correlation measure of association between genes. In this work, we integrate distance correlation and network topological centrality into the reasoning about the structure of gene regulatory networks. We will select optimal thresholds based on the characteristics of the distribution of each gene pair in relation to distance correlation. Experiments were carried out on four network datasets and their performance was compared.
An Experimental and Numerical Study on the Effect of Spacing between Two Helmholtz Resonators
Authors: Gautam Abhishek , Celik Alper , Azarpeyvand Mahdi
Published: February 2021

This study investigates the acoustic performance of a system of two Helmholtz resonators experimentally and numerically. The distance between the Helmholtz resonators was varied to assess its effect on the acoustic performance of the system quantitatively. Experiments were performed using an impedance tube with two instrumented Helmholtz resonators and several microphones along the impedance tube. The relation between the noise attenuation performance of the system and the distance between two resonators is presented in terms of the transmission loss, transmission coefficient, and change in the sound pressure level along the tube. The underlying mechanisms of the spacing effect are further elaborated by studying pressure and the particle velocity fields in the resonators obtained through finite element analysis. The results showed that there might exist an optimum resonators spacing for achieving maximum transmission loss. However, the maximum transmission loss is not accompanied by the broadest bandwidth of attenuation. The pressure field and the sound pressure level spectra of the pressure field inside the resonators showed that the maximum transmission loss is achieved when the resonators are spaced half wavelength of the associated resonance frequency wavelength and resonate in-phase. To achieve sound attenuation over a broad frequency bandwidth, a resonator spacing of a quarter of the wavelength is required, in which case the two resonators operate out-of-phase.
Evaluation of SEBS, METRIC-EEFlux, and QWaterModel Actual Evapotranspiration for a Mediterranean Cropping System in Southern Italy
Authors: Nisa Zaibun , Khan Muhammad Sarfraz , Govind Ajit , Marchetti Marco , Lasserre Bruno , Magliulo Enzo , Manco Antonio
Published: February 2021

Remote sensing-based evapotranspiration (ET) models with various levels of sophistication have emerged recently with the possibilities of user-defined model calibrations. Their application for water resources management and climate studies from regional to global scale has been rapidly increasing, which makes it important to validate field scale ET in a complex crop assemblage before operational use. Based on in situ flux-tower measurements by the eddy-covariance (EC) system, this study tested three single-source energy balance models for estimating daily ET from fennel/maize/ryegrass-clover cropland rotations in a Mediterranean context in southern Italy. The sensitivity of three user-friendly ET models (SEBS, QWaterModel, and METRIC-EEFlux) with reference to the EC system over a center pivot irrigated cropland is discussed in detail. Results in terms of statistical indicators revealed that SEBS and METRIC-EEFlux showed reasonable agreements with measured ET (r2 = 0.59SEBS, RMSE = 0.71 mm day−1; r2 = 0.65METRIC, RMSE = 1.13 mm day−1) in terms of trends and magnitudes. At 30 m spatial resolution, both models were able to capture the in-field variations only during the maize development stage. The presence of spurious scan lines due to sensor defects in Landsat L7 ETM+ can contribute to the qualities of the METRIC-Efflux’s ET product. In our observation, the QWaterModel did not perform well and showed the weakest congruency (r2 = 0.08QWaterModel) with ground-based ET estimates. In a nutshell, the study evaluated these automated remote sensing-based ET estimations and suggested improvements in the context of a generic approach used in their underlying algorithm for robust ET retrievals in rotational cropland ecosystems.
Agronomic Comparisons of Heirloom and Modern Processing Tomato Genotypes Cultivated in Organic and Conventional Farming Systems
Authors: Ronga Domenico , Caradonia Federica , Vitti Antonella , Francia Enrico
Published: February 2021

The yield and fruit quality of processing tomatoes (Solanum lycopersicum L.) have increased markedly over the past decades. The aim of this work was to assess the effects of the organic (OFS) and conventional farming systems (CFS) on the main agronomic parameters involved in processing tomato yield components and fruit quality traits of heirloom and modern genotypes. Marketable yield increased from heirloom to modern genotypes, both in OFS and in CFS, showing a difference of ≈20 t per hectare in favor of CFS. Total fruit yield (TY) was not improved from heirloom to modern assessed genotypes, and a difference of ≈35 t per hectare was observed in favor of CFS. In both farming systems, the highest marketable yield of modern genotypes was due to a higher number of fruits per plant, harvest index, nitrogen agronomic efficiency (NAE), and fruit water productivity. Moreover, the main growth parameters involved in the yield differences between OFS and CFS were the number of leaves per plant, the average fruit weight, the normalized difference vegetation index (NDVI), and NAE. It is noteworthy that fruit quality improvement in terms of color and brix per hectare was paralleled by a decrease of tomato pH in both farming systems. According to our results, we conclude that to reduce the current yield gap between OFS and CFS, agronomic and breeding efforts should be undertaken to increase leaf area index, fruit number per plant, and NAE for better genotype adaptation to organic farming systems.