bluuebunny/crossref_metadata_2025 huggingface dataset:
Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size
Authors: Dunn Clarence A. , Lampe Paul D.
Published: November 2013

The proteins that form vertebrate gap junctions, the connexins, are highly regulated and have short (< 2 h) half-lives. Phosphorylation of connexin43 (Cx43) is generally known to affect gap junction assembly, channel gating and turnover. After finding dramatic effects on gap junctions with Akt inhibitors, we created an antibody specific for Cx43 phosphorylated on S373, a potential Akt substrate. We found S373 phosphorylation in cells and skin or heart almost exclusively in larger gap junctional structures that increased dramatically after wounding or hypoxia. We were able to mechanistically show that Akt-dependent S373 phosphorylation increases gap junction size and communication by completely eliminating interaction between Cx43 and ZO-1. Thus, phosphorylation on S373 acts as a molecular “switch” to rapidly increase gap junctional communication potentially leading to initiation of activation and migration of keratinocytes or ischemic injury response in skin and heart, respectively.
Synthesis of Highly Conjugated Dinuclear Ru Complexes Bridged by a Novel N2–N3 Ligand and Their Application in Photocatalytic Oxygenation of Sulfides
Authors: Kozawa Kazuyuki , Inagaki Akiko , Akita Munetaka
Published: November 2013

Highly conjugated dinuclear Ru complexes bridged by a novel N2–N3 ligand were synthesized. The dinuclear Ru complexes were active toward photoredox-mediated catalytic sulfide oxygenation via proton-coupled oxidation in neutral conditions. Various control experiments have shown that both dinuclear motif and light are essential for the reaction.
Canagliflozin
Authors: Cada Dennis J. , Ingram Kyle T. , Levien Terri L. , Baker Danial E.
Published: November 2013

Each month, subscribers to The Formulary Monograph Service receive 5 to 6 well-documented monographs on drugs that are newly released or are in late phase 3 trials. The monographs are targeted to Pharmacy & Therapeutics Committees. Subscribers also receive monthly 1-page summary monographs on agents that are useful for agendas and pharmacy/nursing in-services. A comprehensive target drug utilization evaluation/medication use evaluation (DUE/MUE) is also provided each month. With a subscription, the monographs are sent in print and are also available on-line. Monographs can be customized to meet the needs of a facility. A drug class review is now published monthly with The Formulary Monograph Service. Through the cooperation of The Formulary, Hospital Pharmacy publishes selected reviews in this column. For more information about The Formulary Monograph Service, call The Formulary at 800-322-4349. The November 2013 monograph topics are dolutegravir, levomilnacipran, ibrutinib, serelaxin, and polynuclear iron (III)-oxyhydroxide (PA21). The DUE/MUE is on dolutegravir.
Circulating U2 Small Nuclear RNA Fragments as a Novel Diagnostic Tool for Patients with Epithelial Ovarian Cancer
Authors: Kuhlmann Jan Dominik , Baraniskin Alexander , Hahn Stephan A , Mosel Frank , Bredemeier Maren , Wimberger Pauline , Kimmig Rainer , Kasimir-Bauer Sabine
Published: November 2013

BACKGROUND

Ovarian cancer is the leading cause of death among malignancies in women. Despite advances in treatment, >50% of patients relapse. For disease monitoring, the identification of a blood-based biomarker would be of prime interest. In this regard, noncoding RNAs, such as microRNA (miRNA) or small nuclear RNA (snRNA), have been suggested as biomarkers for noninvasive cancer diagnosis. In the present study, we sought to identify differentially expressed miRNA/snRNA in sera of ovarian cancer patients and investigate their potential to aid in therapy monitoring.

METHODS

miRNA/snRNA abundance was investigated in serum (n = 10) by microarray analysis and validated in an extended serum set (n = 119) by reverse-transcription quantitative PCR.

RESULTS

Abundance of U2-1 snRNA fragment (RNU2-1f) was significantly increased in sera of ovarian cancer patients (P < 0.0001) and paralleled International Federation of Gynecology and Obstetrics stage as well as residual tumor burden after surgery (P < 0.0001 and P = 0.011, respectively). Moreover, for patients with suboptimal debulking, preoperative RNU2-1f concentration was associated with radiographic response after chemotherapy and with platinum resistance (P = 0.0088 and P = 0.0015, respectively). Interestingly, according to the RNU2-1f abundance dynamics, persistent RNU2-1f positivity before surgery and after chemotherapy identified a subgroup of patients with high risk of recurrence and poor prognosis.

CONCLUSIONS

This is the first report to suggest that a circulating snRNA can serve as an auxiliary diagnostic tool for monitoring tumor dynamics in ovarian cancer. Our results provide a rationale to further investigate whether this high-risk patient group may benefit from additional therapies that are directly applied after chemotherapy.
Supporting the creative industries: The rationale for an exchange of thinking between the art and business schools
Authors: Kearney Gemma , Harris Paul
Published: November 2013

Increasing interest in the concept of the ‘Creative Industries’ with emphasis upon the ‘industry’ aspect, presents questions as to how Art Schools can best prepare students. Indeed the industrial aspect encourages consideration of business and entrepreneurship and by adopting a conceptual approach to draw together different strands of literature from art, design, business and entrepreneurship research, this article explores the issues and challenges in supporting students in a creative context. Areas where an exchange of thinking could occur between the Art and Business Schools are identified and where the cross-fertilization of ideas and teaching practices could offer new methods to support students to engage in the Creative Industries. Barriers remain in teaching entrepreneurship, but viewing it as a process, utilizing the Theory of Effectuation and drawing attention to the prevalence of entrepreneurial teams proffers insights.
The mechanics of managing the Cultural and Creative Industry ParkCultural and Creative Industry Park in National Taiwan University of Arts
Authors: Huang Mei-Hsien
Published: November 2013

In response to the trend of creative economy and in support of the national education policy of university-industry links and the mission of cultivating talents, National Taiwan University of Arts established the first Cultural and Creative Industry Park in Taiwan in 2007. This also qualifies National Taiwan University of Arts as the only university affiliated with a cultural and creative park in Taiwan. This paper aims to explore the mechanics of managing the Cultural and Creative Industry ParkCultural and Creative Industry Park in National Taiwan University of Arts. Through document analysis this article reveals the six operational characteristics of this park, including renovating the old paper mill factory, planning the multifunctional space, inviting excellent cultural and creative manufacturers to station in, establishing a specialized management unit, utilizing university academic and administrative resources, and striving for the government subsidy and industry reward. There are five objectives of the operational content of the Cultural and Creative Industry ParkCultural and Creative Industry Park, including teaching and internship, consultation for starting an enterprise, research innovation, industry incubation, and life aesthetics promotion. Finally, the paper points out three concerns regarding current operations.
Reviews
Authors: Carrier David , Haanstra Folkert , Schönau Diederik
Published: November 2013

World Art: An Introduction to the Art in Artefacts, Ben Burt (2013) London and New York: Bloomsbury Academic, 272 pp., ISBN: 978-1-84788-943-0, p/bk, $29.95, £17.99

Zeichnen: Wahrnehmen, Verarbeiten, Darstellen. Empirische Untersuchungen Zur Ermittlung Räumlich-Visueller Kompetenzen IM Kunstunterricht, Edith Glaser-Henzer, Ludwig Diehl, Luitgard Diehl Ott, Georg Peez (2012) München: Kopaed, 201 pp., ISBN: 978-3-86736-133-0, p/bk, €18.80

Künstlerische Kunstpädagogik. Ein Diskurs Zur Künstlerischen Bildung, Carl-Peter Buschkühle (ed.) (2012) Oberhausen: Athena Verlag, 482 pp., ISBN: 978-3-89896-514-9, p/bk, €29.50
Splassshf: còmic i experimentació narrativa
Authors: Maestre Brotons Antoni
Published: November 2013

Encara que el còmic té una trajectòria centenària, cal esperar la década dels setanta perquè la seua influència es faça notar en la literatura. En aquell moment es produeix el famós debat entre els apocalíptics i els integrats respecte a la cultura de masses apuntat per Umberto Eco, que provocarà en els escriptors més joves el refús a la tradició i als criteris acadèmics sobre excel·lència literària. Els autors més agosarats experimentaran amb els recursos que els gèneres populars poden aportar per a explorar nous estils. Si bé la relació que la literatura estableix amb el cinema ha generat nombrosos estudis, en canvi manquen aproximacions crítiques a la influència que els altres gèneres visuals i audiovisuals com el còmic o la televisió han exercit sobre la narrativa. Aquesta és una qüestió que se situa en l’àmbit dels estudis sobre la interdiscursivitat. Per tant, no pot deixar de banda aspectes com la serialitat, la construcció narrativa basada en la juxtaposició, les onomatopeies, l’humor o els protagonistas inanimats o animals.
15 anys de còmic-vinyetes a la Universitat d’Alacant: Unicòmic com a model acadèmic de didàctica i promoció de la lectura
Authors: Rovira Collado Joan Miquel , Rovira Collado José
Published: November 2013

El 2013 s’organitzaran les XV Jornades del Còmic de la Universitat d’Alacant-Unicòmic, celebrades ininterrompudament des del 1999. D’una petita proposta de l’alumnat, hem passat a unes jornades acadèmiques estables i reconegudes, centrades en les possibilitats didàctiques del nové art. Al llarg de les quinze edicions, hi han desfilat autors de renom internacional com Chris Claremont, Francisco Solano López o Carlos Giménez, a banda de nombrosos especialistes que ens van presentar els seus treballs al voltant del món de la historieta, com Álvaro Pons o Antonio Martín. En el següent estudi, s’hi fa un minuciós recorregut per l’organització i la història de les jornades tot destacant els moments més rellevants i la importància que han tingut aquestes jornades per al reconeixement de la narració gràfica com una forma d’expressió artística que, a l’igual que la literatura, ha de ser analitzada i treballada des de les aules universitàries, ja que ens pot beneficiar en l’ensenyament de molts àmbits d’investigació. Gràcies a Unicòmic, a la Universitat d’Alacant les vinyetes han deixat de considerar-se una distracció juvenil per a considerar-se una forma d’art equiparable a la literatura.
Margarida Xirgu, epistolari íntim
Authors: Foguet i Boreu Francesc
Published: November 2013

La correspondència escrita per l’actriu i directora d’escena Margarida Xirgu (1888-1969), tant la familiar o amical com l’estrictament professional, permet d’aprofundir en la seva vida pública i privada i, especialment, en la xarxa de coneixences i relacions al llarg d’una àmplia trajectòria consagrada en cos i ànima al teatre. A partir d’aquesta documentació epistolar, d’un gran valor biogràfic, històric i documental, el present article se centra, sobretot, en l’anàlisi de dos aspectes. D’una banda, la progressiva emergència d’un jo en les cartes xirguanes, en les quals els temes de l’envelliment i del retorn a la pàtria esdevenen leitmotivs obsessius. De l’altra, l’omnipresència del teatre en les missives, la qual no tan sols confirma la dedicació absorbent als escenaris, sinó que també aporta evidències noves sobre la imatge que s’autoconstruïa com a professional i sobre els seus gustos artístics i afinitats estètiques. No cal dir que l’epistolari de Xirgu revela, de pròpia mà, tot un caràcter i unes preocupacions vitals concretes que contribueixen a conèixer molt millor la biografia de l’actriu catalana.
Ressenya de Figures i esbossos. Estudis sobre literatura valenciana contemporània, de Vicent Salvador (Institut Alacantí de Cultura Juan Gil-Albert, Alacant, 2012, 204 pàgines)
Authors: Martín Escribà Àlex
Published: November 2013

Ressenya de Figures i esbossos. Estudis sobre literatura valenciana contemporània, de Vicent Salvador (Institut Alacantí de Cultura Juan Gil-Albert, Alacant, 2012, 204 pàgines)
Ressenya de Literatura oral a Faió, Favara, Maella i Nonasp, de Carme Oriol, Pere Navarro i Mònica Sales (Associació Cultural del Matarranya, Calaceit, 2010, 272 pàgines)
Authors: Vidal Lloret Vicent
Published: November 2013

Ressenya de Literatura oral a Faió, Favara, Maella i Nonasp, de Carme Oriol, Pere Navarro i Mònica Sales (Associació Cultural del Matarranya, Calaceit, 2010, 272 pàgines).
Heart rate variability in male patients with metabolic syndrome
Authors: Kratnov Andrey Evgen'evich , Yakimova A V , Silkina E E
Published: November 2013

Aim. To study the heart rate variability via 24-hours ECG monitoring in male patients with metabolic syndrome (MS) and no signs of ischemic heart disease (IHD). Materials and Methods. 131 males aged 29 to 60 years with no evidence for IHD were enrolled for this study and underwent 24-hoursECG monitoring procedure. Results. We determined that MS in males is associated with dysautonomia, accompanied by decrease in sympathetic heart stimulation (specifically, in LF and VLF parameters) and left ventricular diastolic abnormalities that correlate with abdominal obesity. Conclusion. MS in males is associated with dysautonomia, accompanied by decrease in sympathetic heart stimulation (specifically, in LF and VLF parameters) and left ventricular diastolic abnormalities that correlate with abdominal obesity.
Transcutaneous oximetry monitoring in patients with type 2 diabetes mellitus and critical limb ischemia
Authors: Bondarenko Olga Nikolaevna , Ayubova N L , Galstyan Gagik Radikovich , Dedov Ivan Ivanovich
Published: November 2013

Aims. To evaluate transcutaneous oximetry as a method for diagnostics and monitoring in patients with diabetes mellitus (DM) and critical limb ischemia (CLI) after percutaneous transluminal balloon angioplasty (PTBA). Materials and Methods. We enrolled 126 patients with DM and CLI for participation in this study (148 limbs in total). 22 patients underwent PTBA on both lower limbs, and 104 ? on single limb. Transcutaneous oximetry and duplex ultrasonography of lower limb arteries was performed prior to PTBA with subsequent examinations on 5-7th days, 1st, 3rd and 6th month after intervention. Transcu- taneous oxygen tension (TcpO2) was measured by Radiometer (Copenhagen) oximeter system. Duplex ultrasonography was performed on Voluson 730? Expert system (GE Medical Systems Kretztechnik GmbHCo OHG, Austria). Results. Multiple factor analysis suggests that results of TcpO2 monitoring prior to and after PTBA are influenced by presence of ischemic heart disease, severe lower limb infections, serum creatinine, arterial hypertension and lower limb reperfusion edema. We observed a strong correlation of TcpO2 with the degree of anterior tibial artery and dorsal pedis artery occlusion. Conclusion. Transcutaneous oximetry allows evaluation of CLI severity and efficiency of PTBA in the majority oа patients with DM and CLI. Certain comorbidities impose limitations on this technique. Efficiency of endovascular intervention should be evaluated based on complex non-invasive examination, clinical data and signs of CLI.
Insulin therapy and body weight: benefits of biphasic human insulin analogue NovoMix 30
Authors: Surkova Elena Viktorovna
Published: November 2013

Body weight (BW) excess is a characteristic problem for type 2 diabetes mellitus (T2DM) both as its pathogenetic feature and as a side effect of blood glucose lowering therapy. In the latter case reduction of glycosuria and frequent hypoglycemic events are primarily blamed for BW gain, but additional factors like direct effect of insulin on lipogenesis and influence of its supraphysiologic levels on regulation of appetite via CNS structures are also under discussion.Advances of the last years have brought new hope due to introduction of drug classes that do not affect BW. However, fraction of patients dependent on exogenous insulin shows stable trend for growth. Deterioration of beta-cell secretory capacity makes insulin an ultimately indispensable tool in the foreseeable future. As so, insulin therapy modalities with minimal impact on BW are preferable. In this regard human insulin analogues of both rapid and prolonged action have certain advantages.Current article addresses influence of pre-mixed insulin preparation NovoMix 30 (Novo Nordisk, Denmark) on BW. A summary of several studies of substantial duration (up to 3 years) suggests a neutral effect on BW in various categories of T2DM patients (including obese and elder patients).Therapy with pre-mixed preparations is an adequately safe and effective T2DM treatment modality and is advantageous for patients in whom BW gain is particularly unfavorable.
Incidence of sleep apnea in patients with various types of glycemic disturbances
Authors: Dreval' Alexander Vasil'evich , Misnikova Inna Vladimirovna , Gubkina V A , Agal'tsov M V , Fedorova S I
Published: November 2013

Aims. To assess the risk for sleep apnea in patients with various types of glycemic disorders by means of Epworth Sleepiness Scale andSleep Apnea Screening Questionnaire. Materials and Methods. We examined 744 residents of Mozhaisk Region, that were considered to have high risk for development of type2 diabetes mellitus (T2DM), as estimated by FINDRISK Questionnaire. Patients, who scored 12+ were cleared for participation in this study. Combined score from Epworth Sleepiness Scale and Sleep Apnea Screening Questionnaire was applied for diagnosis of sleep apnea, supplemented with specific questions about snoring and episodes of apnea. Glycemic disorders were diagnosed with standard glucose tolerance test. Results. 42.7% of examined patients (n=318) were diagnosed with various types of glucose disorders. Prevalence of abdominal obe- sity (according to waist circumference measurement) comprised 59.3% in male patients and 54.1% in females. We observed positive correlation between body mass index (BMI) and snoring ? 0.3 (p=0.0001), BMI and apnea ? 0.2 (p=0.0001), BMI and daytime sleepiness ? 0.1 (p=0.007); we also observed direct correlation between age and snoring ? 0.2 (p=0.0001), as well as age and sleep apnea ? 0.1 (p=0.028). Risk for sleep apnea was found to be 4.7 times higher in patients with arterial hypertension. After adjustment71Диагностика, контроль и лечениеСахарный диабет. 2013;(1):71?77Сахар ный диабетfor age risk of apnea remained 2.8 times higher in patients with T2DM, 1.9 times higher in subjects with impaired glucose tolerance and1.6 times higher in subjects with impaired fasting glycaemia. Relative risk for snoring in patients with various types of glycemic disorders was 1.1-1.2 against normoglycemic controls. We estimated that all types of glycemic disorders increase risk for apnea 1.2?1.6 times. Conclusion. Glycemic disorders, body weight excess, obesity and arterial hypertension are risk factors for snoring and sleep apnea. Corresponding patient categories should be screened for sleep apnea by questionnaire survey to identify those in need of further complex examination and treatment.
Deregulated expression of selected histone methylases and demethylases in prostate carcinoma
Authors: Vieira Filipa Quintela , Costa-Pinheiro Pedro , Ramalho-Carvalho João , Pereira Andreia , Menezes Francisco Duarte , Antunes Luís , Carneiro Isa , Oliveira Jorge , Henrique Rui , Jerónimo Carmen
Published: November 2013

Prostate cancer (PCa), a leading cause of cancer-related morbidity and mortality, arises through the acquisition of genetic and epigenetic alterations. Deregulation of histone methyltransferases (HMTs) or demethylases (HDMs) has been associated with PCa development and progression. However, the precise influence of altered HMTs or HDMs expression and respective histone marks in PCa onset and progression remains largely unknown. To clarify the role of HMTs and HDMs in prostate carcinogenesis, expression levels of 37 HMTs and 20 HDMs were assessed in normal prostate and PCa tissue samples by RT-qPCR.SMYD3,SUV39H2,PRMT6,KDM5A, andKDM6Awere upregulated, whereasKMT2A-E (MLL1-5)andKDM4Bwere downregulated in PCa, compared with normal prostate tissues. Remarkably,PRMT6was the histone modifier that best discriminated normal from tumorous tissue samples. Interestingly,EZH2andSMYD3expression levels significantly correlated with less differentiated and more aggressive tumors. Remarkably,SMYD3expression levels were of independent prognostic value for the prediction of disease-specific survival of PCa patients with clinically localized disease submitted to radical prostatectomy. We concluded that expression profiling of HMTs and HDMs, especiallySMYD3, might be of clinical usefulness for the assessment of PCa patients and assist in pre-therapeutic decision-making.
Implicating SCF Complexes in Organogenesis in Caenorhabditis elegans
Authors: Polley Stanley R G , Kuzmanov Aleksandra , Kuang Jujiao , Karpel Jonathan , Lažetić Vladimir , Karina Evguenia I , Veo Bethany L , Fay David S
Published: November 2013

Development of the Caenorhabditis elegans foregut (pharynx) is regulated by a network of proteins that includes the Retinoblastoma protein (pRb) ortholog LIN-35; the ubiquitin pathway components UBC-18 and ARI-1; and PHA-1, a cytoplasmic protein. Loss of pha-1 activity impairs pharyngeal development and body morphogenesis, leading to embryonic arrest. We have used a genetic suppressor approach to dissect this complex pathway. The lethality of pha-1 mutants is suppressed by loss-of-function mutations in sup-35/ztf-21 and sup-37/ztf-12, which encode Zn-finger proteins, and by mutations in sup-36. Here we show that sup-36 encodes a divergent Skp1 family member that binds to several F-box proteins and the microtubule-associated protein PLT-1/τ. Like SUP-35, SUP-36 levels were negatively regulated by UBC-18–ARI-1. We also found that SUP-35 and SUP-37 physically associated and that SUP-35 could bind microtubules. Thus, SUP-35, SUP-36, and SUP-37 may function within a pathway or complex that includes cytoskeletal components. Additionally, SUP-36 may regulate the subcellular localization of SUP-35 during embryogenesis. We carried out a genome-wide RNAi screen to identify additional regulators of this network and identified 39 genes, most of which are associated with transcriptional regulation. Twenty-three of these genes acted via the LIN-35 pathway. In addition, several S-phase kinase-associated protein (Skp)1–Cullin–F-Box (SCF) components were identified, further implicating SCF complexes as part of the greater network controlling pharyngeal development.
Recombination and Annealing Pathways Compete for Substrates in Making rrn Duplications in Salmonella enterica
Authors: Reams Andrew B , Kofoid Eric , Duleba Natalie , Roth John R
Published: November 2013

Tandem genetic duplications arise frequently between the seven directly repeated 5.5-kb rrn loci that encode ribosomal RNAs in Salmonella enterica. The closest rrn genes, rrnB and rrnE, flank a 40-kb region that includes the purHD operon. Duplications of purHD arise by exchanges between rrn loci and form at a high rate (10−3/cell/division) that remains high in strains blocked for early steps in recombination (recA, recB, and/or recF), but drops 30-fold in mutants blocked for later Holliday junction resolution (ruvC recG). The duplication defect of a ruvC recG mutant was fully corrected by an added mutation in any one of the recA, recB, or recF genes. To explain these results, we propose that early recombination defects activate an alternative single-strand annealing pathway for duplication formation. In wild-type cells, rrn duplications form primarily by the action of RecFORA on single-strand gaps. Double-strand breaks cannot initiate rrn duplications because rrn loci lack Chi sites, which are essential for recombination between two separated rrn sequences. A recA or recF mutation allows unrepaired gaps to accumulate such that different rrn loci can provide single-strand rrn sequences that lack the RecA coating that normally inhibits annealing. A recB mutation activates annealing by allowing double-strand ends within rrn to avoid digestion by RecBCD and provide a new source of rrn ends for use in annealing. The equivalent high rates of rrn duplication by recombination and annealing pathways may reflect a limiting economy of gaps and breaks arising in heavily transcribed, palindrome-rich rrn sequences.
General Triallelic Frequency Spectrum Under Demographic Models with Variable Population Size
Authors: Jenkins Paul A , Mueller Jonas W , Song Yun S
Published: November 2013

It is becoming routine to obtain data sets on DNA sequence variation across several thousands of chromosomes, providing unprecedented opportunity to infer the underlying biological and demographic forces. Such data make it vital to study summary statistics that offer enough compression to be tractable, while preserving a great deal of information. One well-studied summary is the site frequency spectrum—the empirical distribution, across segregating sites, of the sample frequency of the derived allele. However, most previous theoretical work has assumed that each site has experienced at most one mutation event in its genealogical history, which becomes less tenable for very large sample sizes. In this work we obtain, in closed form, the predicted frequency spectrum of a site that has experienced at most two mutation events, under very general assumptions about the distribution of branch lengths in the underlying coalescent tree. Among other applications, we obtain the frequency spectrum of a triallelic site in a model of historically varying population size. We demonstrate the utility of our formulas in two settings: First, we show that triallelic sites are more sensitive to the parameters of a population that has experienced historical growth, suggesting that they will have use if they can be incorporated into demographic inference. Second, we investigate a recently proposed alternative mechanism of mutation in which the two derived alleles of a triallelic site are created simultaneously within a single individual, and we develop a test to determine whether it is responsible for the excess of triallelic sites in the human genome.
Estimation of the Spontaneous Mutation Rate per Nucleotide Site in aDrosophila melanogasterFull-Sib Family
Authors: Keightley Peter D , Ness Rob W , Halligan Daniel L , Haddrill Penelope R
Published: November 2013

We employed deep genome sequencing of two parents and 12 of their offspring to estimate the mutation rate per site per generation in a full-sib family of Drosophila melanogaster recently sampled from a natural population. Sites that were homozygous for the same allele in the parents and heterozygous in one or more offspring were categorized as candidate mutations and subjected to detailed analysis. In 1.23 × 109 callable sites from 12 individuals, we confirmed six single nucleotide mutations. We estimated the false negative rate in the experiment by generating synthetic mutations using the empirical distributions of numbers of nonreference bases at heterozygous sites in the offspring. The proportion of synthetic mutations at callable sites that we failed to detect was <1%, implying that the false negative rate was extremely low. Our estimate of the point mutation rate is 2.8 × 10−9 (95% confidence interval = 1.0 × 10−9 − 6.1 × 10−9) per site per generation, which is at the low end of the range of previous estimates, and suggests an effective population size for the species of ∼1.4 × 106. At one site, point mutations were present in two individuals, indicating that there had been a premeiotic mutation cluster, although surprisingly one individual had a G→A transition and the other a G→T transversion, possibly associated with error-prone mismatch repair. We also detected three short deletion mutations and no insertions, giving a deletion mutation rate of 1.2 × 10−9 (95% confidence interval = 0.7 × 10−9 − 11 × 10−9).
Juxtaposing teatro de revista and cine
Authors: Avila Jacqueline
Published: November 2013

In the early 1900s, Mexico’s popular entertainment spectacle was the teatro de revista, a satirical theatrical genre derived from the Spanish zarzuela that combined social analysis and political commentary with burlesque. Combining monologues and comedic skits with music and dance performances, the revista became an important component in the daily lives of urban Mexicans, placing the popular of contemporary culture center stage. With the advent of sound cinema, several key players from the revista transferred their talents from the stage to the screen and molded a cinematic practice with strong theatrical origins. As a consequence of the crossover, these actors, musicians, and composers kept their fingers on the popular pulse of the nation as the overlap of revista structures into sound film provided an easy and non-intimidating way of enticing the urban audience to screening spaces. For the comedia ranchera (ranch comedy) during the 1930s, the synthesized revista provided a sturdy dramatic foundation, but the development of the film genre depended on musical performance because the protagonist, the singing charro, sang. Rancheras performed by mariachis dressed in full trajes de charros (charro suits) became one of the genre’s musical and visual signifiers, but the comedia ranchera’s musical compilation is much more diverse, juxtaposing several originally composed canciones mexicanas, canciones rancheras and traditional music. This paper examines the comedia ranchera of the 1930s not with the intention of concentrating on a picturesque representation of folklore on screen, but as an experimental product of a specific socio-cultural context and a vehicle of musical hybridization that created a successful cinematic formula for the national industry. I discuss those hybrid components such as the singing charro protagonist, theatrical practice, and melodramatic currents that structured the comedia ranchera, examining in particular Fernando de Fuentes’s groundbreaking Allá en el Rancho Grande (Over on the Big Ranch, 1936).
The Continuity of Melos
Authors: McLucas Anne Dhu
Published: November 2013

The “hurry” is one type of melo found in music for pantomime of the eighteenth century and throughout the nineteenth century in melodrama, after which it was adopted for silent film accompaniment, and then in scoring sound films. The use of “hurries” shows the persistence of this type of melo for action scenes, or to convey a hurried state of mind, in four different theatrical genres, and can still be found in contemporary film scores, if no longer identified by that name. An examination of Thomas Holcroft’s A Tale of Mystery (1802, music by Thomas Busby), an 1880s’ theatrical adaptation of Alexandre Dumas’s Le comte de Monte Cristo (The Count of Monte Cristo), and excerpts from J.S. Zamecnik’s Sam Fox Moving Picture Music (1913) illustrate the continuity of hurry use across two centuries of theatrical melos practice.
Composing with Types and Flexible Modules
Authors: Moormann Peter
Published: November 2013

Creator of more than one hundred film scores, John Williams is one of the world’s most successful film composers. In his long career, Williams (b. 1932) has written music for practically every genre from science fiction to comedy. The question arises: how does he create his highly differentiated scores so quickly? One answer lies in his componential system of riffs and motifs he develops like building blocks—basic patterns that are varied and adapted from film to film, depending upon the specific situation at hand. These building blocks can be traced back to musical idioms originally formed in the nineteenth century, subsequently adapted for silent films and then for talkies.
Melodrama Made Good
Authors: Hannington Luke
Published: November 2013

As one of the last great stage melodramas, Edgar Stillman Kelley’s Score to Ben-Hur represents one of the last flourishes of melodramatic music before the onset of the movie age. This article provides an overview of the music in the autograph score of Ben-Hur and explores the various dramatic functions of the music as presented by Kelley.
Enfermagem neonatal: o sentido existencial do cuidado na unidade de terapia intensiva
Authors: Melo Rita de Cássia de Jesus , Souza Ívis Emília de Oliveira , Paula Cristiane Cardoso de
Published: November 2013

Entender que cuidado ao recém-nascido prematuro perpassa por diversas ações que estão, por vezes, imbricadas na rotina predominante nas Unidades de Terapia Intensiva Neonatal, requer que profissionais compreendam a sutileza entre o cuidado no sentido de atividades e procedimentos realizados no dia-a-dia, e cuidado no sentido existencial de ser e cuidar do outro. Este estudo, na abordagem fenomenológica heideggeriana, objetivou desvelar o sentido existencial do cuidado às mães de bebês prematuros internados em UTIN. Após aprovação pelo Comitê de Ética em Pesquisa, foram realizadas entrevistas com nove mães. A análise pelo método heideggeriano desvelou que o ser-mãe, ao ser-com-o-filho, também se sente cuidada pelos profissionais que propiciam que enfrente esse momento existencial de maneira mais segura.
Fragilidade da rede social de famílias de crianças com doença crônica
Authors: Araújo Yana Balduíno , Reichert Altamira Pereira da Silva , Vasconcelos Maria Gorete Lucena de , Collet Neusa
Published: November 2013

A doença crônica na infância afeta as relações sociais familiares. A rede social pode contribuir para um melhor enfrentamento ao fornecer apoio. Objetivou-se analisar as fragilidades da rede social de famílias de crianças com doença crônica. Trata-se de uma pesquisa qualitativa, realizada em um hospital público da Paraíba entre abril e junho de 2011, utilizando genograma, ecomapa e entrevista semiestruturada com sete familiares de crianças com doença crônica. A análise temática dos resultados possibilitou caracterizar a rede e apreender as interações sociais na trajetória da doença e os tipos de apoio recebidos. A categoria empírica (des)articulação da rede social de famílias de crianças com doença crônica e as modificações na rotina da família foi construída nesse processo e apontou a diversidade da composição da rede social, evidenciando que o apoio social fornecido pela rede é significativo, porém nem sempre é contínuo e condizente com as necessidades das famílias.
Avaliação da acupressão auricular na síndrome do ombro doloroso: estudo de caso
Authors: Zanelatto Ana Paula
Published: November 2013

A Síndrome do Ombro Doloroso (SOD) é definida como dor e limitação funcional decorrente do acometimento de estruturas estáticas e dinâmicas do ombro, como ligamentos, cápsula e músculos. É uma das queixas mais comuns e incapacitantes do sistema musculoesquelético na população em geral com uma prevalência estimada entre 15 a 25%. Este estudo objetivou avaliar os resultados da acupressão auricular quando usada como terapia no tratamento da Dor crônica, secundária à SOD, quanto ao efeito analgésico e terapêutico satisfatório e o tempo estimado de tratamento. Para a obtenção do objetivo foi realizado um Estudo de Caso que teve como intervenção a acupressão auricular com esferas de cristais e como indicador de resultado o questionário Penn Shoulder Score (PSS - Brasil). Os dados foram analisados qualitativa e quantitativamente. Conclui-se que a auriculoterapia é uma importante técnica terapêutica, pois o estudo demonstrou uma melhora na pontuação total do PSS - Brasil em 34,3%.
Estresse ocupacional em enfermeiros intensivistas que prestam cuidados diretos ao paciente crítico
Authors: Inoue Kelly Cristina , Versa Gelena Lucinéia Gomes da Silva , Murassaki Ana Cláudia Yassuko , Melo Willian Augusto de , Matsuda Laura Misue
Published: November 2013

Realizou-se pesquisa descritivo-exploratória em cinco instituições hospitalares da região Oeste do estado do Paraná, entre maio e julho de 2010, com o objetivo de identificar o nível de estresse em enfermeiros intensivistas que prestam cuidados diretos a pacientes críticos. Participaram 58 enfermeiros intensivistas assistenciais, cujo nível de estresse foi avaliado de acordo com o Domínio D da Escala Bianchi de Estresse. A maioria (65,5%) apresentou nível de estresse médio. Os estressores com maior pontuação foram Enfrentar a morte (5,6 pontos); Atender às emergências na unidade (5,1 pontos); Atender os familiares de pacientes críticos ou Orientar familiares de pacientes críticos (ambos com 4,8 pontos). É preciso adotar estratégias para a prevenção / redução de estresse, porque os principais estressores apontados relacionam-se a atribuições específicas dos enfermeiros.
Classificação internacional de funcionalidade, incapacidade e saúde: utilização no cuidado de enfermagem a pessoas idosas
Authors: Santos Silvana Sidney Costa , Lopes Manuel José , Vidal Danielle Adriane Silveira , Gautério Daiane Porto
Published: November 2013

Objetivou-se refletir acerca da Classificação Internacional de Funcionalidade, Incapacidade e Saúde e sua utilização no cuidado de enfermagem às pessoas idosas. Realizou-se breve historicidade sobre a Classificação; abordou-se a avaliação funcional do idoso e sua importância para o planejamento adequado às ações cuidativas; apresenta-se a aplicabilidade da classificação internacional de funcionalidade, incapacidade e saúde por enfermeiros. A Classificação tem condições de direcionar a avaliação funcional da pessoa idosa, pelo enfermeiro, tornando-a integral, percebendo este ser humano como possuidor de um corpo, de necessidades de realizar atividades e de participação, além de pertencer a um contexto/ambiente.
Dos gêneros textuais utilizados na formação do professor de Biologia
Authors: Oliveira Odisséa Boaventura de , Trivelato Silvia Luiza Frateschi
Published: November 2013

Problematiza-se nesta pesquisa os gêneros relatório e artigo científico escritos por 4 estudantes- estagiários na disciplina Prática de Ensino de Biologia, tomando-se as funções enunciativas locutor, enunciador e autor, conforme explicitadas por Orlandi (2003). Considera-se o locutor aquele que se representa como 'eu' no discurso; o enunciador é o que representa as 'perspectivas que o eu assume' no discurso e autor, quando se assume enquanto 'produtor' da linguagem, portanto afetado pelo contato com o social e suas coerções. A análise dos textos escritos aponta que tais gêneros não contribuíram expressivamente para manifestações subjetivas do ser e estar professor, por conta disso propõe-se a escrita de textos que levem o autor a se identificar com aquilo que escreve deixando ali suas marcas subjetivas.
Adolescent's motivation and their perceptions of learning contexts of foreign language
Authors: Cavenaghi Ana Raquel Abelha , Bzuneck José Aloyseo , Rufini Sueli Édi
Published: November 2013

The aim of the present study was to investigate motivational orientations of 396 adolescents of public schools and their perceptions of their learning contexts. About half of the sample suited English as a compulsory language, while others studied Spanish as an elected language. Data were collected through a Likert-type self report questionnaire with questions related to mastery and work avoidance achievement goals, to perceptions of interesting classes and utility value. Results showed significant positive relations among mastery goal orientation and perceptions of interesting classes and utility, and negative relations among work avoidance goal orientation and those variables. Regression analyses showed that perceptions of interesting classes and utility value predict mastery goal orientation. Some significant differences aroused among students of the two learning contexts. Results were discussed in the light of motivational theories and some suggestions for new researches and for educational practices as well with adolescents were drawn.
Contribuições de Vigotski para a compreensão do psiquismo
Authors: Souza Vera Lucia Trevisan de , Andrada Paula Costa de
Published: November 2013

Este artigo é resultado de muitas leituras, estudos, reflexões e debates de um grupo de pesquisa que toma a Psicologia Histórico-Cultural como aporte teórico e metodológico. Sua natureza é teórica, visto que decorre dos escritos de alguns de seus estudiosos para apresentar compreensões das ideias centrais de Vigotski, principal teórico da referida abordagem. Objetivamos reunir e discutir conceitos-chave desse autor, os quais se encontram dispersos em sua obra, com o intuito de apresentar suas possibilidades de interpretação e aplicação em estudo e práticas sobre o desenvolvimento humano. A complexidade da obra do autor demanda inúmeras investigações devido ao caráter dialético e complexo de suas postulações. Buscamos, assim, demonstrar neste texto a plausibilidade de sua teoria para explicar os fenômenos psicológicos, oferecendo contribuições e sugerindo ações que possam respaldar a prática do psicólogo em contextos institucionais, principalmente a escola.
A perversão, o desejo e o gozo: articulações possíveis
Authors: Santos Adelson Bruno dos Reis , Besset Vera Lopes
Published: November 2013

Os autores procuram subsidiar aportes para uma discussão acerca da especificidade estrutural da perversão e sua relação com o desejo e com o gozo. Circunscrever o campo da perversão a partir de sua etiologia, delimitar os processos metapsicológicos capazes de objetivá-la e definir suas características específicas contrapostas às da neurose e da psicose, desde Freud, não constitui tarefa fácil. Ao estudar esse controverso construto teórico-clínico, os autores acentuam o quanto a perversão, na maioria das vezes, aparece como um conceito fronteiriço, tênue e de referências confusas no que diz respeito à sua especificidade estrutural. A proposta é avançar para além dos paradigmas freudianos, tendo como pano de fundo a dialética do desejo no ensino de Lacan. Existiria um caminho propiciador de uma articulação possível entre o desejo e o gozo?
The new Adoption Law: legal and psychological aspects
Authors: Oliveira Débora Silva de , Schwartz Eda Regina Doederlein
Published: November 2013

From a legal and psychological standpoint, this article examines some aspects involved in adoption, including the effects of the alterations made to the Statute of Children and Adolescents by the National Adoption Law. It verifies whether or not the Law accelerated the performance of adoption processes. This paper is based on bibliographic searches and statistics from Rio Grande do Sul. Results show that the Law was innovative in many aspects but ceased to tackle others, such as adoption by homosexuals. Proceedings have become more bureaucratic, and the number of adoptions in the state, according to the records, remains insignificant. The New Law has not yet achieved its objective of accelerating the adoption process. Institutional sheltering, which should be temporary, at times becomes permanent, which contradicts the right of children/adolescents to family life and results in significant psychological damage to them.
Influences internationales sur la lexicographie pedagogique du fle
Authors: Binon J. , Verlinde S. , Selva T.
Published: November 2013

L´article commence par dresser la liste des cinq fonctions principales qu´un dictionnaire d´(auto-)apprentissage doit assumer : réception ; production ; (auto-)apprentissage ; dimension lexiculturelle et médiation entre L1 et L2, L3, etc.. En passant en revue les dictionnaires de langue française récents, les auteurs révèlent les carences et les lacunes des dictionnaires de français langue maternelle Ils mentionnent également les influences internationales et les caractéristiques de dictionnaires pour apprenants de langue étrangère, élaborés dans d´autres pays. Sont présentées ensuite les innovations principales introduites par le DAFA ( Dictionnaire d´Apprentissage du Français des Affaires, version papier et électronique) et le DAFLES (Dictionnaire d´Apprentissage du Français Langue Etrangère ou Seconde), qui n'existe pour l'instant que sous forme électronique, consultable en ligne. Après se trouvent analysées les conditions pour pratiquer la lexicographie pédagogique. Dans les conclusions les auteurs soulignent les relations entre la lexicographie en langue maternelle et en langue étrangère et l´importance d´initier les apprenants de langues à la manipulation de dictionnaires, à l´analyse des différents types de dictionnaires et à leur structure. Enfin les auteurs évoquent les défis que doit relever le lexicographe qui souhaite réaliser un dictionnaire d´ (auto-) apprentissage.
Dilemas da literalidade na tradução juramentada
Authors: Aubert Francis Henrik
Published: November 2013

A condição "juramentada" da tradução executada por tradutores públicos tende a exigir a adoção de uma postura literalizante, centrada no texto de partida. A natureza dos textos comumente submetidos a esse tipo de tradução tende a exigir uma abordagem comunicativa, substituindo os "modos de dizer" do original pelos "modos de dizer" correntes na comunidade de chegada. Para averiguar de que modo os tradutores públicos lidam com estas demandas conflitantes, analisou-se as tradução de quatro fraseologismos por doze tradutores públicos. Os dados coligidos sugerem uma certa flutuação nas soluções encontradas, a relevância da interlingua como espaço de conciliação das dificuldades tradutórias, bem como uma correlação entre o grau de especificidade (lingüística ou cultural) e o coeficiente de dispersão nas soluções tradutórias propostas.
Os alicerces sociopolíticos do cooperativismo de crédito rural solidário na Zona da Mata de Minas Gerais
Authors: Freitas Alair Ferreira de , Freitas Alan Ferreira de
Published: November 2013

O objetivo deste artigo é analisar processos e estruturas sociais que sustentaram e condicionaram a constituição e a atuação das cooperativas de crédito vinculadas ao sistema Ecosol na Zona da Mata Mineira. A coleta de dados ocorreu em 2010 nos municípios onde a Ecosol possui unidades de atendimento: Araponga, Tombos, Espera Feliz, Divino e Simonésia, por meio de observação participante, análise documental e aplicação de entrevistas semiestruturadas. Os principais atores e fatores identificados como alicerces sociopolíticos dessas cooperativas foram: mística e religiosidade, introduzida por setores da Igreja Católica, que interferiu diretamente nas representações sociais e manifestações simbólicas que orientaram as intervenções para uma mudança da realidade; a política, marcada pelo movimento sindical e partidário (Partido dos Trabalhadores); e a técnica e estilo de vida, consolidados a partir da adoção de um projeto de agricultura sustentável e desenvolvimento alternativo introduzida por uma ONG.
Assimetria de transmissão de preço na comercialização da uva fina de mesa no Paraná: 1997 a 2011
Authors: Alves Alexandre Florindo , Tonin Julyerme Matheus , Carrer Marcelo José
Published: November 2013

O Paraná se destaca como um dos principais produtores de uva fina de mesa no Brasil, tendo como característica a presença de pequenos e médios produtores. O presente estudo objetiva analisar a assimetria na transmissão de preço entre os níveis produtor, atacado e varejo da uva fina de mesa no Paraná, no período de janeiro de 1997 a outubro de 2011. A metodologia empregada para verificar como ocorre a transmissão de preços entre os agentes foi o Vetor Autorregressivo (VAR). O modelo utilizado para mensurar Assimetria de Transmissão de Preços (ATP) foi baseado na metodologia desenvolvida por Grififth e Piggott (1994). Os principais resultados foram: na análise de transmissão de preço ao varejo, o atacado apresentou um coeficiente de elasticidade maior do que o nível do produtor; o sentido unicausal atacado-varejo do teste de Granger é uma evidência de presença de informações assimétricas; e os acréscimos dos preços são transmitidos com mais rapidez do que os decréscimos, que, junto aos resultados do teste t de Griffith e Piggott (1994), comprova a existência de assimetria na transmissão de preços.