bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential and Therapeutic Efficacy of Cell-based Therapy Using Mesenchymal Stem Cells for Acute/chronic Kidney Disease
Authors: Yun Chul Won , Lee Sang Hun
Published: April 2019

Kidney disease can be either acute kidney injury (AKI) or chronic kidney disease (CKD) and it can lead to the development of functional organ failure. Mesenchymal stem cells (MSCs) are derived from a diverse range of human tissues. They are multipotent and have immunomodulatory effects to assist in the recovery from tissue injury and the inhibition of inflammation. Numerous studies have investigated the feasibility, safety, and efficacy of MSC-based therapies for kidney disease. Although the exact mechanism of MSC-based therapy remains uncertain, their therapeutic value in the treatment of a diverse range of kidney diseases has been studied in clinical trials. The use of MSCs is a promising therapeutic strategy for both acute and chronic kidney disease. The mechanism underlying the effects of MSCs on survival rate after transplantation and functional repair of damaged tissue is still ambiguous. The paracrine effects of MSCs on renal recovery, optimization of the microenvironment for cell survival, and control of inflammatory responses are thought to be related to their interaction with the damaged kidney environment. This review discusses recent experimental and clinical findings related to kidney disease, with a focus on the role of MSCs in kidney disease recovery, differentiation, and microenvironment. The therapeutic efficacy and current applications of MSC-based kidney disease therapies are also discussed.
Interferon-Stimulated Genes—Mediators of the Innate Immune Response during Canine Distemper Virus Infection
Authors: Klotz Daniela , Gerhauser Ingo
Published: April 2019

The demyelinating canine distemper virus (CDV)-leukoencephalitis represents a translational animal model for multiple sclerosis. The present study investigated the expression of type I interferon (IFN-I) pathway members in CDV-induced cerebellar lesions to gain an insight into their role in lesion development. Gene expression of 110 manually selected genes in acute, subacute and chronic lesions was analyzed using pre-existing microarray data. Interferon regulatory factor (IRF) 3, IRF7, signal transducer and activator of transcription (STAT) 1, STAT2, MX protein, protein kinase R (PKR), 2′-5′-oligoadenylate synthetase (OAS) 1 and interferon-stimulated gene (ISG) 15 expression were also evaluated using immunohistochemistry. Cellular origin of STAT1, STAT2, MX and PKR were determined using immunofluorescence. CDV infection caused an increased expression of the antiviral effector proteins MX, PKR, OAS1 and ISG15, which probably contributed to a restricted viral replication, particularly in neurons and oligodendrocytes. This increase might be partly mediated by IRF-dependent pathways due to the lack of changes in IFN-I levels and absence of STAT2 in astrocytes. Nevertheless, activated microglia/macrophages showed a strong expression of STAT1, STAT2 and MX proteins in later stages of the disease, indicating a strong activation of the IFN-I signaling cascade, which might be involved in the aggravation of bystander demyelination.
Electrospun Water-Borne Polyurethane Nanofibrous Membrane as a Barrier for Preventing Postoperative Peritendinous Adhesion
Authors: Chen Shih-Heng , Chou Pang-Yun , Chen Zhi-Yu , Lin Feng-Huei
Published: April 2019

Peritendinous adhesion is a major complication after tendon injury and the subsequent repairs or reconstructions. The degree of adhesion can be reduced by the interposition of a membranous barrier between the traumatized tendon and the surrounding tissue. In the present study, electrospun water-borne polyurethane (WPU) nanofibrous membranes (NFMs) were created for use after the reparation or reconstruction of tendons to reduce adhesion. In the electrospinning process, water was employed as the solvent for WPU, and this solvent was ecofriendly and nontoxic. The nanofibrous architecture and pore size of the WPU NFMs were analyzed. Their microporosity (0.78–1.05 µm) blocked the penetration of fibroblasts, which could result in adhesion and scarring around the tendon during healing. The release of WPU mimicked the lubrication effect of the synovial fluid produced by the synovium around the tendon. In vitro cell studies revealed that the WPU NFMs effectively reduced the number of fibroblasts that became attached and that there was no significant cytotoxicity. In vivo studies with the rabbit flexor tendon repair model revealed that WPU NFMs reduced the degree of peritendinous adhesion, as determined using a gross examination; a histological cross section evaluation; and measurements of the range of motion of interphalangeal joints (97.1 ± 14.7 and 79.0 ± 12.4 degrees in proximal and distal interphalangeal joints respectively), of the length of tendon excursion (11.6 ± 1.9 cm), and of the biomechanical properties.
The Dual-Specificity Phosphatase 10 (DUSP10): Its Role in Cancer, Inflammation, and Immunity
Authors: Jiménez-Martínez Marta , Stamatakis Konstantinos , Fresno Manuel
Published: April 2019

Cancer is one of the most diagnosed diseases in developed countries. Inflammation is a common response to different stress situations including cancer and infection. In those processes, the family of mitogen-activated protein kinases (MAPKs) has an important role regulating cytokine secretion, proliferation, survival, and apoptosis, among others. MAPKs regulate a large number of extracellular signals upon a variety of physiological as well as pathological conditions. MAPKs activation is tightly regulated by phosphorylation/dephosphorylation events. In this regard, the dual-specificity phosphatase 10 (DUSP10) has been described as a MAPK phosphatase that negatively regulates p38 MAPK and c-Jun N-terminal kinase (JNK) in several cellular types and tissues. Several studies have proposed that extracellular signal-regulated kinase (ERK) can be also modulated by DUSP10. This suggests a complex role of DUSP10 on MAPKs regulation and, in consequence, its impact in a wide variety of responses involved in both cancer and inflammation. Here, we review DUSP10 function in cancerous and immune cells and studies in both mouse models and patients that establish a clear role of DUSP10 in different processes such as inflammation, immunity, and cancer.
Using Long-term Capture Data to Predict Trogoderma variabile Ballion and Plodia interpunctella (Hübner) Population Patterns
Authors: Gerken Alison R. , Campbell James F.
Published: April 2019

Insects can infest facilities that house and process post-harvest grains and grain-based products. Integrated pest management tactics rely on tracking insect populations and using this information to select and target management tactics. Our ability to predict when and where to best focus treatment relies on an understanding of long-term trends, but often any available monitoring data are limited in its duration. Here we present data collected over a 10-year period at a flour mill in the central part of the United States. Using traps placed both inside and outside a flour mill and baited with pheromone-lures for Plodia interpunctella (Hübner), Indianmeal moth, and Trogoderma variabile Ballion, warehouse beetle, we examine environmental and spatial variability in insect captures. We find that both species, inside and outside the mill, are highly influenced by seasonal patterns, with peaks of insect captures during the warm season (April through September). There is also consistency across time and space in trap capture for P. interpunctella with traps in an open location consistently capturing high numbers of insects. In contrast, T. variabile lacked consistency in trap capture but were most often not found in the same trap locations as P. interpunctella. Fumigations conducted within the facility appeared to have little impact on insect captures inside, with dynamics appearing to be driven more by broader seasonal patterns in activity. These data and analyses suggest that there is a larger population of these insects that are readily moving in and out of the structures, while fumigation treatments are only impacting a small portion of the overall population and tactics targeting immigration may be an important addition to the pest management program.
Residual Efficacy of a Deltamethrin Emulsifiable Concentrate Formulation against Rhyzopertha dominica (F.) and Sitotroga cerealella (Oliver) after Partial Treatment of Brown Rice
Authors: Arthur Frank H.
Published: April 2019

Rhyzopertha dominica (Fab.), the lesser grain borer, and Sitotroga cerealella (Olivier), the Angoumois grain moth, are internally feeding stored product insects that can infest raw grains. In this test, brown rice was treated with 0.5 and 1.0 ppm of a new emulsifiable concentrate (EC) formulation of the pyrethroid deltamethrin and stored for 12 months. One day after treatment, and every 3 months for 12 months, treated rice was mixed with untreated brown rice in the following ratios: 0:100 (untreated controls), 10:90, 25:75, 50:50, 75:25, and 100:0 (all treated). Bioassays were conducted by exposing 10 parental adults of each species on the rice mixtures and assessing progeny production, feeding damage, and weight loss. The progeny of S. cerealella ranged from 105.6 F1 adults on untreated brown rice to 69.4 F1 adults on 100% treated rice, but there was little feeding damage or weight loss. The progeny production of R. dominica declined from 177.4 F1 adults on untreated rice to 9.8 F1 adults on 100% treated rice. Weight loss and feeding damage were correlated with progeny production. The results show that the new deltamethrin formulation could be used for protection of brown rice, but S. cerealella may be less susceptible to deltamethrin compared to R. dominica.
Experimental Investigation of the Magnetoelectric Effect in NdFeB-Driven A-Line Shape Terfenol-D/PZT-5A Structures
Authors: Zhang Juanjuan , Kang Yan , Gao Yuanwen , Weng George J.
Published: April 2019

In this paper, the magnetoelectric (ME) effect is investigated in two kinds of A-line shape Terfenol-D/PZT-5A structures by changing the position of the NdFeB permanent magnet. The experimental results show that both ME composite structures had multiple resonance peaks. For the ME structure with acrylonitrile-butadiene-styrene (ABS) trestles, the resonance peak was different for different places of the NdFeB permanent magnet. Besides, the maximum of the ME coefficient was 4.142 V/A at 32.2 kHz when the NdFeB permanent magnet was on top of the Terfenol-D layer. Compared with the ME coefficient with a DC magnetic field, the ME coefficient with NdFeB magnets still maintained high values in the frequency domain of 65~87 kHz in the ME structure with mica trestles. Through Fourier transform analysis of the transient signal, it is found that the phenomenon of multiple frequencies appeared at low field frequency but not at high field frequency. Moreover, the output ME voltages under different AC magnetic fields are shown. Changing the amplitude of AC magnetic field, the magnitude of the output voltage changed, but the resonant frequency did not change. Finally, a finite element analysis was performed to evaluate the resonant frequency and the magnetic flux distribution characteristics of the ME structure. The simulation results show that the magnetic field distribution on the surface of Terfenol-D is non-uniform due to the uneven distribution of the magnetic field around NdFeB. The resonant frequencies of ME structures can be changed by changing the location of the external permanent magnet. This study may provide a useful basis for the improvement of the ME coefficient and for the optimal design of ME devices.
Amphiphilic Copolymer of Polyhedral Oligomeric Silsesquioxane (POSS) Methacrylate for Solid Dispersion of Paclitaxel
Authors: Chatterjee Suchismita , Ooya Tooru
Published: April 2019

Suitable polymers for the homogeneous formulation of drug/polymer mixtures should be selected to correct the structural and physicochemical nature with a rapid dissolution rate. This study aimed to evaluate a copolymer prepared by the radical polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and a polyhedral oligomeric silsesquioxane (POSS) methacrylate bearing an ethyl (C2H5) group (MPC-ran-C2H5-POSS) as a carrier for the solid formulation of paclitaxel (PTX). A single-phase homogeneous formulation of PTX with the mixture of the MPC-ran-C2H5-POSS and polyvinylpyrrolidone (PVP) was prepared by a solvent method. The formulation of MPC-ran-C2H5-POSS/PVP/PTX enhanced the dissolution rate and the dissolved amount (approximately 90% within 40 min) without precipitation. The X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC) analysis confirmed the presence of PTX as an amorphous state. The amphiphilic nature of the MPC-ran-C2H5-POSS contributed to enhancing the aqueous solubility of PTX. The new formulation is applicable for solid dispersion technique via the supersaturation of PTX in an aqueous media.
Four Questions in Cellular Material Design
Authors: Bhate Dhruv
Published: April 2019

The design of cellular materials has recently undergone a paradigm shift, enabled by developments in Additive Manufacturing and design software. No longer do cellular materials have to be limited to traditional shapes such as honeycomb panels or stochastic foams. With this increase in design freedom comes a significant increase in optionality, which can be overwhelming to the designer. This paper aims to provide a framework for thinking about the four key questions in cellular material design: how to select a unit cell, how to vary cell size spatially, what the optimal parameters are, and finally, how best to integrate a cellular material within the structure at large. These questions are posed with the intent of stimulating further research that can address them individually, as well as integrate them in a systematic methodology for cellular material design. Different state-of-the-art solution approaches are also presented in order to provoke further investigation by the reader.
Designing and Testing Cold-Formed Rounded Connections Made on a Prototype Station
Authors: Rejek Michał , Wróbel Nikodem , Królczyk Jolanta , Królczyk Grzegorz
Published: April 2019

This paper presents the design of cold-formed rounded connections between a tube and a connecting block and the analysis of test results that were carried out with six fabricated samples. The joints manufactured on a specially designed prototype station were made by forming tools that were adjusted to connecting elements regarding the diameter and the shape. All of the samples prepared for this study were of the same diameters relating to the diameter of a hole in a connecting block and the outer diameter of a pipe flange. However, they were different concerning the height of the connecting block flange. The article presents features of joints that were manufactured with a designed forming tool on the prototype station. The achieved connections were examined in destructive testing (Micrography, Tensile Strength Test) and in non-destructive testing (Leakage Test). The research project aims were to state the differences in energy consumption of made connections and extend the concept of cold-formed rounded connections. Furthermore, this article presents the effects of FEA simulation of the cold-formed joint based on the results of destructive and non-destructive tests.
Fluid Cartilage as New Autologous Biomaterial in the Treatment of Minor Nose Defects: Clinical and Microscopic Difference Amongst Diced, Crushed, and Fluid Cartilage
Authors: Trivisonno Angelo , Cohen Steven R. , Magalon Guy , Magalon Jèrèmy , Sterodimas Aris , Pascali Michele , Cervelli Valerio , Toietta Gabriele , Colaprietra Alfredo , Calcagni Filippo , Orlandi Augusto , Scioli Maria Giovanni , Gentile Pietro
Published: April 2019

Developing cartilage constructs with injectability, appropriate matrix composition, and persistent cartilaginous phenotype remains an enduring challenge in cartilage repair. Fourteen patients with minor contour deformity were treated with fluid cartilage filler gently injected as autologous fluid graft in deep planes of defect of the nose that were close to the bone or the cartilage. A computerized tomographic scan control was performed after 12 months. Pearson’s Chi-square test was used to investigate differences in cartilage density between native and newly formed cartilages. The endpoints were the possibility of using fluid cartilage as filler with aesthetic and functional improvement and versatility. Patients were followed up for two years. The constructs of fluid cartilage graft that were injected in the deep plane resulted in a persistent cartilage tissue with appropriate morphology, adequate central nutritional perfusion without central necrosis or ossification, and further augmented nasal dorsum without obvious contraction and deformation. This report demonstrated that fluid cartilage grafts are useful for cartilage regeneration in patients with outcomes of rhinoplasty, internal nasal valve collapse, and minor congenital nose aesthetics deformity.
Light-Emitting Porphyrin Derivative Obtained from a Subproduct of the Cashew Nut Shell Liquid: A Promising Material for OLED Applications
Authors: de Amorim Lima Nayane Maria , Camargo Avila Harold José , do Nascimento Marchiori Cleber Fabiano , Gondim Sampaio Samuel , Ferreira Mota João Paulo , Gomes Pereira Ribeiro Viviane , da Silva Clemente Claudenilson , Mele Giuseppe , Cremona Marco , Mazzetto Selma Elaine
Published: April 2019

In this work, the meso-tetra[4-(2-(3-n-pentadecylphenoxy)ethoxy]phenylporphyrin (H2P), obtained from the cashew nut shell liquid (CNSL), and its zinc (ZnP) and copper (CuP) metallic complexes, were applied as emitting layers in organic light emitting diodes (OLEDs). These compounds were characterized via optical and electrochemical analysis and the electroluminescent properties of the device have been studied. We performed a cyclic voltammetry analysis to determine the Highest Occupied Molecular Orbital (HOMO) and Lowest Unoccupied Molecular Orbital (LUMO) energy levels for the porphyrins, in order to select the proper materials to assemble the device. H2P and ZnP presented fluorescence emission band in the red region, from 601 nm to 718 nm. Moreover, we verified that the introduction of bulky substituents hinders the π–π stacking, favoring the emission in the film. In addition, the strongest emitter, ZnP, presented a threshold voltage of 4 V and the maximum irradiance of 10 μW cm−2 with a current density (J) of 15 mA cm−2 at 10 V. The CuP complex showed to be a favorable material for the design of OLEDs in the infrared. These results suggest that the porphyrins derived from a renewable source, such as CNSL, is a promising material to be used in organic optoelectronic devices such as OLEDs.
Fluorescence-Based Analysis of Noncanonical Functions of Aminoacyl-tRNA Synthetase-Interacting Multifunctional Proteins (AIMPs) in Peripheral Nerves
Authors: Kim Muwoong , Kim Hyosun , Kim Dokyoung , Park Chan , Huh Youngbuhm , Jung Junyang , Chung Hyung-Joo , Jeong Na Young
Published: April 2019

Aminoacyl-tRNA synthetase-interacting multifunctional proteins (AIMPs) are auxiliary factors involved in protein synthesis related to aminoacyl-tRNA synthetases (ARSs). AIMPs, which are well known as nonenzymatic factors, include AIMP1/p43, AIMP2/p38, and AIMP3/p18. The canonical functions of AIMPs include not only protein synthesis via multisynthetase complexes but also maintenance of the structural stability of these complexes. Several recent studies have demonstrated nontypical (noncanonical) functions of AIMPs, such as roles in apoptosis, inflammatory processes, DNA repair, and so on. However, these noncanonical functions of AIMPs have not been studied in peripheral nerves related to motor and sensory functions. Peripheral nerves include two types of structures: peripheral axons and Schwann cells. The myelin sheath formed by Schwann cells produces saltatory conduction, and these rapid electrical signals control motor and sensory functioning in the service of survival in mammals. Schwann cells play roles not only in myelin sheath formation but also as modulators of nerve degeneration and regeneration. Therefore, it is important to identify the main functions of Schwann cells in peripheral nerves. Here, using immunofluorescence technique, we demonstrated that AIMPs are essential morphological indicators of peripheral nerve degeneration, and their actions are limited to peripheral nerves and not the dorsal root ganglion and the ventral horn of the spinal cord.
Improvement of Compressibility and Thaw-Settlement Properties of Warm and Ice-Rich Frozen Soil with Cement and Additives
Authors: Chai Mingtang , Zhang Jianming
Published: April 2019

The warm and ice-rich frozen soil (WIRFS) that underlies roadway embankments in permafrost regions exhibit large compression and thaw deformation, which can trigger a series of distresses. Cement and additives were used in this study to improve the compressibility and thaw-settlement properties of WIRFS. We, therefore, selected optimum additives and studied the improvement effect on the frozen soil with 30% water content based on our previous research. Given constant load and variable temperatures, compression coefficients, thaw strains, and water content changes were obtained at temperatures of −1.0 °C, −0.5 °C, and 2.0 °C to evaluate the effect of improvements. A scanning electron microscope (SEM) was then used to observe the microstructure of improved soils and analyze causal mechanisms. Data show that hydration reactions, physical absorptions, cement, and additives formed new structures and changed the phase of water in frozen soil after curing at −1.0 °C for 28 days. This new structure, cemented with soil particles, unfrozen water, and ice, filled in the voids of frozen soil and effectively decreased the WIRFS compression coefficient and thaw strain.
Bending Properties of Zigzag-Shaped 3D Woven Spacer Composites: Experiment and FEM Simulation
Authors: Zhu Liming , Lyu Lihua , Zhang Xuefei , Wang Ying , Guo Jing , Xiong Xiaoqing
Published: April 2019

Conventionally laminated spacer composites are extensively applied in many fields owing to their light weight. However, their impact resistance, interlaminar strength, and integrity are poor. In order to overcome these flaws, the zigzag-shaped 3D woven spacer composites were rationally designed. The zigzag-shaped 3D woven spacer fabrics with the basalt fiber filaments tows 400 tex (metric count of yarn) used as warp and weft yarns were fabricated on a common loom with low-cost processing. The zigzag-shaped 3D woven spacer composites were obtained using the VARTM (vacuum-assisted resin transfer molding) process. The three-point bending deformation and effects of damage in zigzag-shaped 3D woven spacer composites were studied both in experiment and using the finite element method (FEM). The bending properties of zigzag-shaped 3D woven spacer composites with different direction, different numbers of weaving cycle, and different heights were tested in experiments. In FEM simulation, the geometrical model was established to analyze the deformation and damage based on the 3D woven composite structure. Compared with data obtained from the experiments and FEM simulation, the results show good agreement and also prove the validity of the model. Based on the FEM results, the deformation, damage, and propagation of stress obtained from the model are very helpful in analyzing the failure mechanism of zigzag-shaped 3D woven composites. Furthermore, the results can significantly guide the fabrication process of real composite materials.
Influence of Boron Additions and Heat Treatments on the Fatigue Resistance of CoCrMo Alloys
Authors: Hernandez-Rodriguez Marco A. L. , Mercado-Solis Rafael D. , Presbítero Gerardo , Lozano Diego E. , Martinez-Cazares Gabriela M. , Bedolla-Gil Yaneth
Published: April 2019

Cobalt-based alloys are widely used in the manufacture of joint prostheses. In this study, the effect of boron additions and heat treatment on the ASTM F75 was evaluated by rotating bending fatigue. The boron ranged from 0.06–1 wt %. The alloys were tested in as-cast and heat-treated conditions. In the as-cast condition, the infinite life was observed at 380 MPa, improving to 433–615 MPa according to the amount of boron added. In the heat treatment condition, the fatigue resistance was improved only in the base alloy. The addition of 0.06 wt % boron and heat treatment led to the same resistance as in the as-cast condition. Adding large amounts of boron combined with heat treatment diminished the fatigue limit. The fracture analysis revealed primarily brittle behaviour with some ductile features even on the same sample; only the heat-treated alloy with 0.06 wt % boron was clearly ductile. This alloy also exhibited notably better toughness to crack propagation.
In Vitro and In Silico Approaches to Generating and Identifying Angiotensin-Converting Enzyme I Inhibitory Peptides from Green Macroalga Ulva lactuca
Authors: Garcia-Vaquero Marco , Mora Leticia , Hayes Maria
Published: April 2019

A protein extract was generated from the macroalga Ulva lactuca, which was subsequently hydrolysed using the food-grade enzyme papain and angiotensin-converting Enzyme I and renin inhibitory peptides identified using a combination of enrichment strategies employing molecular weight cutoff filtration and mass spectrometry analysis. The generated hydrolysates with the most promising in vitro activity were further purified using preparative RP-HPLC and characterised. The 1 kDa hydrolysate (1 kDa-UFH), purified and collected by preparative RP-HPLC at minutes 41‒44 (Fr41‒44), displayed statistically higher ACE-I inhibitory activities ranging from 96.91% to 98.06%. A total of 48 novel peptides were identified from these four fractions by LC-MS/MS. A simulated gastrointestinal digestion of the identified peptide sequences was carried out using in silico enzyme cleavage simulation tools, resulting in 86 peptide sequences that were further assessed for their potential activity, toxicity and allergenicity using multiple predictive approaches. All the peptides obtained in this study were predicted to be non-toxic. However, 28 out of the 86 novel peptides released after the in silico gastrointestinal digestion were identified as potential allergens. The potential allergenicity of these peptides should be further explored to comply with the current labelling regulations in formulated food products containing U. lactuca protein hydrolysates.
The Importance of Inhaler Adherence to Prevent COPD Exacerbations
Authors: Jardim Jose R. , Nascimento Oliver A.
Published: April 2019

It has been shown that the better outcomes of chronic obstructive pulmonary disease (COPD) are closely associated with adherence to drug therapy, independent of the treatment administered. The clinical trial Towards a Revolution in COPD Health (TORCH) study clearly showed in a three year follow up that patients with good adherence to their inhaler treatment presented a longer time before the first exacerbation, a lower susceptibility to exacerbation and lower all-cause mortality. The Latin American Study of 24-h Symptoms in Chronic Obstructive Pulmonary Disease (LASSYC), a real-life study, evaluated the self-reported inhaler adherence in COPD patients in seven countries in a cross-sectional non-interventional study and found that approximately 50% of the patients had good adherence, 30% moderate adherence and 20% poor adherence. Adherence to inhaler may be evaluated by the specific inhaler adherence questionnaire, the Test of Adherence to Inhalers (TAI). Several factors may predict the incorrect use of inhalers or adherence in COPD outpatient, including the number of devices and the daily dosing frequency. Ideally, patient education, simplicity of the device operation, the use of just one device for multiple medications and the best adaptation of the patient to the inhaler should guide the physician in prescribing the device.
Micro Milled Microfluidic Photoionization Detector for Volatile Organic Compounds
Authors: Rezende Gustavo C. , Le Calvé Stéphane , Brandner Jürgen J. , Newport David
Published: April 2019

Government regulations and environmental conditions are pushing the development of improved miniaturized gas analyzers for volatile organic compounds. One of the many detectors used for gas analysis is the photoionization detector (PID). This paper presents the design and characterization of a microfluidic photoionization detector (or µPID) fabricated using micro milling and electrical discharge machining techniques. This device has no glue and facilitates easy replacement of components. Two materials and fabrication techniques are proposed to produce a layer on the electrodes to protect from ultraviolet (UV) light and possible signal noise generation. Three different microchannels are tested experimentally and their results are compared. The channel with highest electrode area (31.17 mm²) and higher volume (6.47 µL) produces the highest raw signal and the corresponding estimated detection limit is 0.6 ppm for toluene without any amplification unit.
Design Optimization of Double-Gate Isosceles Trapezoid Tunnel Field-Effect Transistor (DGIT-TFET)
Authors: Gu Hwa Young , Kim Sangwan
Published: April 2019

Recently, tunnel field-effect transistors (TFETs) have been regarded as next-generation ultra-low-power semi-conductor devices. To commercialize the TFETs, however, it is necessary to improve an on-state current caused by tunnel-junction resistance and to suppress a leakage current from ambipolar current (IAMB). In this paper, we suggest a novel TFET which features double gate, vertical, and trapezoid isosceles channel structure to solve the above-mentioned technical issues. The device design is optimized by examining its electrical characteristics with the help of technology computer-aided design (TCAD) simulation. As a result, double-gate isosceles trapezoid (DGIT) TFET shows a much better performance than the conventional TFET in terms of ON-state current (ION), IAMB, and gate-to-drain capacitance (CGD). It is confirmed that an inverter composed of DGIT-TFETs can operate with less than 1 ns intrinsic delay time and negligible voltage overshoot.
Magnetically Aligned Nanorods in Alginate Capsules (MANiACs): Soft Matter Tumbling Robots for Manipulation and Drug Delivery
Authors: Mair Lamar , Chowdhury Sagar , Paredes-Juarez Genaro , Guix Maria , Bi Chenghao , Johnson Benjamin , English Bradley , Jafari Sahar , Baker-McKee James , Watson-Daniels Jamelle , Hale Olivia , Stepanov Pavel , Sun Danica , Baker Zachary , Ropp Chad , Raval Shailesh , Arifin Dian , Bulte Jeff , Weinberg Irving , Evans Emily , Cappelleri David
Published: April 2019

Soft, untethered microrobots composed of biocompatible materials for completing micromanipulation and drug delivery tasks in lab-on-a-chip and medical scenarios are currently being developed. Alginate holds significant potential in medical microrobotics due to its biocompatibility, biodegradability, and drug encapsulation capabilities. Here, we describe the synthesis of MANiACs—Magnetically Aligned Nanorods in Alginate Capsules—for use as untethered microrobotic surface tumblers, demonstrating magnetically guided lateral tumbling via rotating magnetic fields. MANiAC translation is demonstrated on tissue surfaces as well as inclined slopes. These alginate microrobots are capable of manipulating objects over millimeter-scale distances. Finally, we demonstrate payload release capabilities of MANiACs during translational tumbling motion.
Carbon-Based Materials for Humidity Sensing: A Short Review
Authors: Tulliani Jean-Marc , Inserra Barbara , Ziegler Daniele
Published: April 2019

Humidity sensors are widespread in many industrial applications, ranging from environmental and meteorological monitoring, soil water content determination in agriculture, air conditioning systems, food quality monitoring, and medical equipment to many other fields. Thus, an accurate and reliable measurement of water content in different environments and materials is of paramount importance. Due to their rich surface chemistry and structure designability, carbon materials have become interesting in humidity sensing. In addition, they can be easily miniaturized and applied in flexible electronics. Therefore, this short review aims at providing a survey of recent research dealing with carbonaceous materials used as capacitive and resistive humidity sensors. This work collects some successful examples of devices based on carbon nanotubes, graphene, carbon black, carbon fibers, carbon soot, and more recently, biochar produced from agricultural wastes. The pros and cons of the different sensors are also discussed in the present review.
The Effect of Inlet Velocity on the Separation Performance of a Two-Stage Hydrocyclone
Authors: Jiang Lanyue , Liu Peikun , Zhang Yuekan , Yang Xinghua , Wang Hui
Published: April 2019

The “entrainment of coarse particles in overflow” and the “entrainment of fine particles in underflow” are two inevitable phenomena in the hydrocyclone separation process, which can result in a wide product size distribution that does not meet the requirement of a precise classification. Hence, this study proposed a two-stage (TS) hydrocyclone, and the effects of the inlet velocity on the TS hydrocyclone were investigated using computational fluid dynamics (CFD). More specifically, the influences of the first-stage inlet velocity on the second-stage swirling flow field and the separation performance were studied. In addition, the particle size distribution of the product was analyzed. It was found that the first-stage overflow contained few coarse particles above 40 μm and that the second-stage underflow contained few fine particles. The second-stage underflow was free of particles smaller than 10 μm and almost free of particles smaller than 20 μm. The underflow product contained few fine particles. Moreover, the median particle size of the second-stage overflow product was similar to that of the feed. Inspired by this observation, we propose to recycle the second-stage overflow to the feed for re-classification and to use only the first-stage overflow and the second-stage underflow as products. In this way, fine particle products free of coarse particle entrainment, and coarse particle products free of fine particle entrainment can be obtained, achieving the goal of precise classification.
Effects of High-Order Simulations on the Simultaneous Stochastic Optimization of Mining Complexes
Authors: de Carvalho Joao Pedro , Dimitrakopoulos Roussos
Published: April 2019

A mining complex is composed of mines, mineral processing streams, stockpiles, and waste facilities, which culminate with generated products that are delivered to customers. The supply uncertainty and variability of materials extracted from the mines, which flow through a mining complex to generate products, can be quantified through geostatistical simulations and can be used as inputs to the simultaneous optimization of mining complexes. A critical aspect to consider is that mineral deposits are characterized by spatially complex, non-Gaussian geological properties and multiple-point connectivity of high-grades, features that are not captured by conventional second-order simulation methods. This paper investigates the benefits of simultaneously optimizing a mining complex where the simulations of the mineral deposit are generated by a high-order, direct-block simulation approach. The optimized life-of-mine (LOM) production schedule is compared to a case in which the same setting is optimized by having the related simulations generated using a second-order simulation method. The comparison shows that the incorporation of simulations that reproduce the spatial connectivity of high-grades results in a more informed LOM production schedule. The sequence of extraction is driven by the spatial connectivity of high-grades, resulting in a mill throughput with better material quality and reduced waste extraction. Furthermore, the discounted cash-flow increases by more than 5% as compared to the case in which the second-order simulations are used.
A UHPLC-UV Method Development and Validation for Determining Kavalactones and Flavokavains in Piper methysticum (Kava)
Authors: Tang Yijin , Fields Christine
Published: April 2019

An ultra-high-performance liquid chromatographic (UHPLC) separation was developed for six kava pyrones (methysticin, dihydromethysticin (DHM), kavain, dihydrokavain (DHK), desmethoxyyangonin (DMY), and yangonin), two unidentified components, and three Flavokavains (Flavokavain A, B, and C) in Piper methysticum (kava). The six major kavalactones and three flavokavains are completely separated (Rs > 1.5) within 15 min using a HSS T3 column and a mobile phase at 60 °C. All the peaks in the LC chromatogram of kava extract or standard solutions were structurally confirmed by LC-UV-MS/MS. The degradations of yangonin and flavokavains were observed among the method development. The degradation products were identified as cis-isomerization by MS/MS spectra. The isomerization was prevented or limited by sample preparation in a non-alcoholic solvent or with no water. The method uses the six kava pyrones and three flavokavains as external standards. The quantitative calibration curves are linear, covering a range of 0.5–75 μg/mL for the six kava pyrones and 0.05–7.5 μg/mL for the three flavokavains. The quantitation limits for methysticin, DHM, kavain, DHK, DMY, and yangonin are approximately 0.454, 0.480, 0.277, 0.686, 0.189, and 0.422 μg/mL. The limit of quantification (LOQs) of the three flavokavains are about 0.270, 0.062, and 0.303 μg/mL for flavokavain C (FKC), flavokavain A (FKA), and flavokavain B (FKB). The average recoveries at three different levels are 99.0–102.3% for kavalactones (KLs) and 98.1–102.9% for flavokavains (FKs). This study demonstrates that the method of analysis offers convenience and adequate sensitivity for determining methysticin, DHM, kavain, DHK, yangonin, DMY, FKA, FKB, and FKC in kava raw materials (root and CO2 extract) and finished products (dry-filled capsule and tablet).
Fabrication and Characterization of MSQ Aerogel Coating on ePTFE Thin Films for Cable Sheaths
Authors: Guo Xingzhong , Bai Shengchi , Shan Jiaqi , Lei Wei , Ding Ronghua , Zhang Yun , Yang Hui
Published: April 2019

With methylsilsesquioxane (MSQ) aerogels synthesized by the sol-gel method as a raw material and Si-Ti sol as a binder, an alcohol-based aerogel slurry consisting of only MSQ aerogel and Si-Ti sol was prepared and coated on expanded polytetrafluoroethylene (ePTFE) to form an MSQ aerogel coating layer, followed by low-temperature heat treatment. The effect of Si-Ti sol content on the microstructure of the MSQ aerogel coating layer was investigated, and the properties of a typical MSQ aerogel-layer-coated ePTFE film were evaluated. The results show that Si-Ti sol has an important role in terms of film-forming capability, surface smoothness, flexibility, and powder dropping of the MSQ aerogel coating layer. With a Si-Ti sol of 10.5 wt.% content as a binder and after heat treatment at 170 °C for 30 min, the coated ePTFE flexible thin film with a layer thickness of 30 μm shows high uniformity, integrity, and electrical insulation properties, with an elongation at break decrease over 130%, a thermal conductivity of 0.1753 W/(m·K) at 25 °C, a dielectric constant of 16.5674, and a dielectric loss of 0.06369, which can be promisingly applied in cable sheaths.
3-Hydroxyflavones and 3-Hydroxy-4-oxoquinolines as Carbon Monoxide-Releasing Molecules
Authors: Soboleva Tatiana , Berreau Lisa M.
Published: April 2019

Carbon monoxide-releasing molecules (CORMs) that enable the delivery of controlled amounts of CO are of strong current interest for applications in biological systems. In this review, we examine the various conditions under which CO is released from 3-hydroxyflavones and 3-hydroxy-4-oxoquinolines to advance the understanding of how these molecules, or derivatives thereof, may be developed as CORMs. Enzymatic pathways from quercetin dioxygenases and 3-hydroxy-4-oxoquinoline dioxygenases leading to CO release are examined, along with model systems for these enzymes. Base-catalyzed and non-redox-metal promoted CO release, as well as UV and visible light-driven CO release from 3-hydroxyflavones and 3-hydroxy-4-oxoquinolines, are summarized. The visible light-induced CO release reactivity of recently developed extended 3-hydroxyflavones and a 3-hydroxybenzo[g]quinolone, and their uses as intracellular CORMs, are discussed. Overall, this review provides insight into the chemical factors that affect the thermal and photochemical dioxygenase-type CO release reactions of these heterocyclic compounds.
Quantification of Furosine (Nε-(2-Furoylmethyl)-l-lysine) in Different Parts of Velvet Antler with Various Processing Methods and Factors Affecting Its Formation
Authors: Gong Rui-ze , Wang Yan-hua , Gao Kun , Zhang Lei , Liu Chang , Wang Ze-shuai , Wang Yu-fang , Sun Yin-shi
Published: April 2019

Furosine (Nε-(2-furoylmethyl)-l-lysine) is formed during the early stages of the Maillard reaction from a lysine Amadori compound and is frequently used as a marker of reaction progress. Furosine is toxic, with significant effects on animal livers, kidneys, and other organs. However, reports on the formation of furosine in processed velvet antler are scarce. In this study, we have quantified the furosine content in processed velvet antler by using UPLC-MS/MS. The furosine contents of velvet antler after freeze-drying, boiling, and processing without and with blood were 148.51–193.93, 168.10–241.22, 60.29–80.33, and 115.18–138.99 mg/kg protein, respectively. The factors affecting furosine formation in processed velvet antler, including reducing sugars, proteins, amino acids, and process temperature, are discussed herein. Proteins, amino acids, and reducing sugars are substrates for the Maillard reaction and most significantly influence the furosine content in the processed velvet antler. High temperatures induce the production of furosine in boiled velvet antler but not in the freeze-dried samples, whereas more furosine is produced in velvet antler processed with blood, which is rich in proteins, amino acids, and reducing sugars, than in the samples processed without blood. Finally, wax slices rich in proteins, amino acids, and reducing sugars produced more furosine than the other parts of the velvet antler. These data provide a reference for guiding the production of low-furosine velvet antler and can be used to estimate the consumer intake of furosine from processed velvet antler.
Antiproliferative Aspidosperma-Type Monoterpenoid Indole Alkaloids from Bousigonia mekongensis Inhibit Tubulin Polymerization
Authors: Zhang Yu , Goto Masuo , Oda Akifumi , Hsu Pei-Ling , Guo Ling-Li , Fu Yan-Hui , Morris-Natschke Susan L. , Hamel Ernest , Lee Kuo-Hsiung , Hao Xiao-Jiang
Published: April 2019

Monoterpenoid indole alkaloids are structurally diverse natural products found in plants of the family Apocynaceae. Among them, vincristine and its derivatives are well known for their anticancer activity. Bousigonia mekongensis, a species in this family, contains various monoterpenoid indole alkaloids. In the current study, fourteen known aspidosperma-type monoterpenoid indole alkaloids (1–14) were isolated and identified from a methanol extract of the twigs and leaves of B. mekongensis for the first time. Among them, compounds 3, 6, 9, and 13 exhibited similar antiproliferative activity spectra against A549, KB, and multidrug-resistant (MDR) KB subline KB-VIN cells with IC50 values ranging from 0.5–0.9 μM. The above alkaloids efficiently induced cell cycle arrest at the G2/M phase by inhibiting tubulin polymerization as well as mitotic bipolar spindle formation. Computer modeling studies indicated that compound 7 likely forms a hydrogen bond (H-bond) with α- or β-tubulin at the colchicine site. Evaluation of the antiproliferative effects and SAR analysis suggested that a 14,15-double bond or 3α-acetonyl group is critical for enhanced antiproliferative activity. Mechanism of action studies demonstrated for the first time that compounds 3, 4, 6, 7, and 13 efficiently induce cell cycle arrest at G2/M by inhibiting tubulin polymerization by binding to the colchicine site.
Application of the N-Dibenzyl Protective Group in the Preparation of β-Lactam Pseudopeptides
Authors: Frlan Rok , Hrast Martina , Gobec Stanislav
Published: April 2019

Despite the great importance of β-lactam antibiotics, there is still a limited number of synthetic approaches for the formation of β-lactam–containing dipeptides. In this study, we report upon the stereoselective preparation of β-lactam–containing pseudopeptides, where different reaction conditions and NH2 protective groups were tested to obtain compounds that contain 3-amino-azetidin-2-one. We demonstrate that the protective group is essential for the outcome of the reaction. Successful implementation of dibenzyl-protected serine-containing dipeptides through the Mitsunobu reaction can provide the desired products at high yields and stereoselectivity.
Effect of 2″-O-Rhamnosyl Icariside II, Baohuoside I and Baohuoside II in Herba Epimedii on Cytotoxicity Indices in HL-7702 and HepG2 Cells
Authors: Zhang Lin , Wang Ting , Zhao Bao-Sheng , Zhang Jing-Xuan , Yang Song , Fan Chun-Lan , Li Pin
Published: April 2019

Herba Epimedii, a commonly used Chinese medicine, has attracted much attention recently because of its potential hepatotoxic effects. 2″-O-Rhamnosyl icariside II, baohuoside I and baohuoside II are the main components of Herba Epimedii, and previous research indicates that these three compounds are related to the hepatotoxicity of Herba Epimedii. To test this idea, in this study, HL-7702 and HepG2 cells were chosen as the in vitro models and the influences of these three compounds on a series of cytotoxicity indices, including ALT, AST, LDH, SOD, GSH, MDA, ROS and MMP, were determined. The results showed that at certain concentrations, the three compounds had different effects on the indices. Among them, baohuoside I at high concentration (32 μg/mL) displayed more significant cytotoxicity than the other two compounds; therefore, it was inferred to be more closely correlated with the liver injury induced by Herba Epimedii combined with the previous study, and its toxic mechanisms may be involved in increasing oxidative stress and inducing apoptosis. The findings of this study may provide evidence of the toxic composition of Herba Epimedii to preliminarily discuss the toxic mechanisms and provide improved guidance for its clinical safety.
Design, Synthesis, and In Vitro Biological Activities of a Bio-Oxidizable Prodrug to Deliver Both ChEs and DYRK1A Inhibitors for AD Therapy
Authors: Barré Anaïs , Azzouz Rabah , Gembus Vincent , Papamicaël Cyril , Levacher Vincent
Published: April 2019

Despite their side effects, cholinesterase (ChE) inhibitors remain the only approved drugs to treat Alzheimer’s disease patients, along with the N-methyl-d-aspartate (NMDA) receptor antagonist memantine. In the last few years, the dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) has also been studied as a promising target for the development of new drugs for this pathology. In this context, and based on our previous characterization of bio-oxidizable prodrugs of potent acetylcholinesterase (AChE) inhibitors, we envisioned a strategy involving the synthesis of a bio-oxidizable prodrug of both ChE and DYRK1A inhibitors. To this end, we fixed our interest on a known potent inhibitor of DYRK1A, namely INDY. The designed prodrug of both ChE and DYRK1A inhibitors was successfully synthesized, connecting both inhibitors by a carbonate link. This prodrug and its corresponding drug were then evaluated as ChEs and DYRK1A inhibitors. Remarkably, in vitro results were in accordance with the starting hypothesis, showing a relative inactivity of the prodrug against DYRK1A and ChEs and a potent inhibition of ChEs by the oxidized form. Molecular docking and kinetic studies of ChE inhibition by the active compound are also discussed in this report.