bluuebunny/crossref_metadata_2025 huggingface dataset:
Where is an emotion? Using targeted visceroception as a method of improving emotion regulation in healthy participants to inform suicide prevention initiatives: a randomised controlled trial
Authors: Davey Steven , Bell Elliot , Halberstadt Jamin , Collings Sunny
Published: July 2020

Background

William James’ 1884 paper “What is an emotion?” has generated much recent interest in affective science regarding somatic contributions to emotion. Studies of interoception (“sensing the physiological condition of the body”) suggest that sensing specific parts of the body contributes to the production of emotion, namely when sensing the viscera (i.e. “visceroception” of the heart, gut or lungs). Improved visceroception has, for instance, been linked to increased emotional intensity, suggesting a role for interoception in emotion regulation that may pertain specifically to visceral bodily locations. Thus, in addition to asking James’ question, “Whatis an emotion?”, we ask, “Whereis an emotion?”. Further, there is an evidence base pointing to the connections between emotion regulation and suicide, and between interoception and suicide. This is a preliminary trial investigating whether targeted interoception/visceroception improves emotion regulation. Ultimately, the overall project aims to inform suicide prevention efforts.

Methods

The trial utilises a pre-test/post-test control group design, with two experimental groups undergoing visceroceptive interventions (focussing on areas pertaining to the gut or heart) and a control group. The interventions will run for 8 weeks. A spatial cueing task will measure reaction times to bodily changes relating to lower abdomen or chest focus. A stop/signal task will measure emotional inhibition, which is hypothesised to obscure awareness of active bodily locations. Visceroceptive ability will be tracked using a heartbeat estimation task, a water load test, and by self-report questionnaire. The sample will consist of healthcare professionals and healthcare students. Despite these being groups that represent a relatively high suicide risk among professional and student groups, all participants will be healthy, given the preliminary nature of this trial.

Discussion

To our knowledge, this will be the first project to address whether emotional feeling presents as a localised bodily phenomenon and whether trained awareness of emotional localisation can improve emotion regulation. It will also be the first to investigate relationships between interoception and emotional inhibition (i.e. whether a sustained interoceptive practice leads to the disinhibition of bodily emotional sensations, which can positively contribute to emotion regulation). These empirical findings on emotion regulation from a healthy sample will be used to inform a desk-based enquiry into the role of embodied emotion in suicide prevention, which may make a significant contribution to a growing evidence base on interoception and suicide.

Trial registration

ACTRN12619000324112. Registered on 4 March 2019. Universal Trial Number (UTN): U1111-1221-0201.
Correction to: ChemoPROphyLaxIs with hydroxychloroquine For covId-19 infeCtious disease (PROLIFIC) to prevent covid-19 infection in frontline healthcare workers: A structured summary of a study protocol for a randomised controlled trial
Authors: McEniery Carmel M. , Fisk Marie , Miles Karen , Kaloyirou Fotini , Hubsch Annette , Smith Jane , Wilkinson Ian B. , Cheriyan Joseph
Published: July 2020

An amendment to this paper has been published and can be accessed via the original article.
Large-scale countrywide screening for tick-borne pathogens in field-collected ticks in Latvia during 2017–2019
Authors: Capligina Valentina , Seleznova Maija , Akopjana Sarmite , Freimane Lauma , Lazovska Marija , Krumins Rudolfs , Kivrane Agnija , Namina Agne , Aleinikova Darja , Kimsis Janis , Kazarina Alisa , Igumnova Viktorija , Bormane Antra , Ranka Renate
Published: July 2020

Background

Tick-borne diseases are of substantial concern worldwide in both humans and animals. Several hard tick species are of medical and veterinary interest in Europe, and changes in the range of tick species can affect the spread of zoonotic pathogens. The aim of the present study was to map the current prevalence and distribution pattern of ticks and related tick-borne pathogens in Latvia, a Baltic state in northern Europe.

Methods

Nearly 4600 Ixodes ricinus, I. persulcatus and Dermacentor reticulatus tick samples were collected in all regions of Latvia during 2017–2019 and were screened by molecular methods to reveal the prevalence and distribution pattern of a wide spectrum of tick-borne pathogens.

Results

New localities of D. reticulatus occurrence were found in western and central Latvia, including the Riga region, indicating that the northern border of D. reticulatus in Europe has moved farther to the north. Among the analyzed ticks, 33.42% carried at least one tick-borne pathogen, and 5.55% of tick samples were positive for two or three pathogens. A higher overall prevalence of tick-borne pathogens was observed in I. ricinus (34.92%) and I. persulcatus (31.65%) than in D. reticulatus (24.2%). The molecular analysis revealed the presence of tick-borne encephalitis virus, Babesia spp., Borrelia spp., Anaplasma phagocytophilum and Rickettsia spp. Overall, 15 and 7 tick-borne pathogen species were detected in Ixodes spp. and D. reticulatus ticks, respectively. This is the first report of Borrelia miyamotoi in Latvian field-collected ticks.

Conclusions

This large-scale countrywide study provides a snapshot of the current distribution patterns of Ixodes and Dermacentor ticks in Latvia and gives us a reliable overview of tick-borne pathogens in Latvian field-collected ticks.
NAFLD as a continuum: from obesity to metabolic syndrome and diabetes
Authors: Godoy-Matos Amélio F. , Silva Júnior Wellington S. , Valerio Cynthia M.
Published: July 2020

Background

The prevalence of non-alcoholic fatty liver disease (NAFLD) has been increasing rapidly. It is nowadays recognized as the most frequent liver disease, affecting a quarter of global population and regularly coexisting with metabolic disorders such as type 2 diabetes, hypertension, obesity, and cardiovascular disease. In a more simplistic view, NAFLD could be defined as an increase in liver fat content, in the absence of secondary cause of steatosis. In fact, the clinical onset of the disease is a much more complex process, closely related to insulin resistance, limited expandability and dysfunctionality of adipose tissue. A fatty liver is a main driver for a new recognized liver-pancreatic α-cell axis and increased glucagon, contributing to diabetes pathophysiology.

Main text

This review will focus on the clinical and pathophysiological connections between NAFLD, insulin resistance and type 2 diabetes. We reviewed non-invasive methods and several scoring systems for estimative of steatosis and fibrosis, proposing a multistep process for NAFLD evaluation. We will also discuss treatment options with a more comprehensive view, focusing on the current available therapies for obesity and/or type 2 diabetes that impact each stage of NAFLD.

Conclusion

The proper understanding of NAFLD spectrum—as a continuum from obesity to metabolic syndrome and diabetes—may contribute to the early identification and for establishment of targeted treatment.
Human adult and adolescent biodistribution and dosimetry of the synaptic vesicle glycoprotein 2A radioligand 11C-UCB-J
Authors: Bini Jason , Holden Daniel , Fontaine Kathryn , Mulnix Tim , Lu Yihuan , Matuskey David , Ropchan Jim , Nabulsi Nabeel , Huang Yiyun , Carson Richard E.
Published: July 2020

The ability to quantify synaptic density in vivo in human adults and adolescents is of vital importance to understanding neuropsychiatric disorders. Here, we performed whole-body scans to determine organ radiation dosimetry of 11C-UCB-J in humans.

Methods

Dynamic whole-body PET scans were performed in four healthy adults after injection of 11C-UCB-J. Regions of interest (ROIs) were drawn manually for the brain, heart, stomach, kidneys, liver, pancreas, spleen, gallbladder, lungs, urinary bladder, and intestines. ROIs were applied to dynamic images to generate time-activity curves (TACs). Decay correction was removed from TACs, and the area under the curve (AUC) for each ROI was calculated. AUCs were then normalized by injected activity and organ volumes to produce radioligand residence times for each organ. These times were then used as input into the OLINDA/EXM 1.0 software to determine the total radiation dose in each organ and the effective dose for these OLINDA models: 55-kg female, 70-kg male, and 15-year-old adolescent.

Results

Visual evaluation detected high uptake in the liver, brain, gallbladder, gastrointestinal tract, and urinary bladder. The dose-limiting organ was the urinary bladder for adult males (0.0224 mSv/MBq) and liver for adult females (0.0248 mSv/MBq) with single-study dose limits of 2239 MBq and 2017 MBq 11C-UCB-J, respectively. For adolescents, the large intestine was the dose-limiting organ (0.0266 mSv/MBq) with a single-study dose limit of 188 MBq.

Conclusions

11C-UCB-J dosimetry in adults is consistent with those for many carbon-11-labeled ligands. Overall, 11C-UCB-J can be used safely in adolescents, as in adults, to measure synaptic density in various neuropsychiatric and other relevant disorders.
Anchor-free object detection with mask attention
Authors: Yang He , Fan Beibei , Guo Lingling
Published: July 2020

The anchor-free method based on key point detection has made great progress. However, the anchor-free method is too dependent on using a convolutional network to generate a rough heatmap. This is difficult to detect for objects with a large size variation and dense and overlapping objects. To solve this problem, first, we propose a mask attention mechanism for object detection methods and make full use of the advantages of the attention mechanism to improve the accuracy of network detection heatmap generation. Then, we designed an optimized fire model to reduce the size of the model. The fire model is an extension of grouped convolution. The fire model allows each group of convolutional network features to learn the same feature through purposeful grouping. In this paper, the mask attention mechanism uses object segmentation images to guide the generation of corner heatmaps. Our approach achieved an accuracy of 91.84% and a recall of 89.83% in the Tencent-100 K dataset. Compared with the popular object detection methods, the proposed method has advantages in model size and accuracy.
The association between diagnosis disclosure and adherence to antiretroviral therapy among adolescents living with HIV in sub-Saharan Africa: a protocol for systematic review and meta-analysis
Authors: Mengesha Melkamu Merid , Ajema Dessalegn , Teshome Awugchew , Tura Abera Kenay
Published: July 2020

Introduction

More than eight in ten of the world’s 1.65 million adolescents living with human immunodeficiency virus (ALHIV) live in sub-Saharan Africa (SSA). Suboptimal adherence to antiretroviral therapy (ART) and poor viral suppression are reported among ALHIV which may in turn compromise the gains achieved so far. The evidence on whether knowing one’s own human immunodeficiency virus (HIV) status and self-disclosure to others benefit adherence to ART or not is inconclusive. This review aims to estimate the association between knowing one’s HIV status and self-disclosure on adherence to ART among ALHIV in SSA.

Methods

Comprehensive search strings will be used to identify relevant observational studies published in English up to May 2020 in major databases: Excerpta Medica database (EMBASE), PubMed, and Ovid/MEDLINE. To access African studies and also to freely access subscription-based articles, the African Index Medicus (AIM) and the WHO HINARI databases will be searched. The AfroLib database will be searched to access the gray literature of African studies. We will use the COVIDENCE software for title/abstract screening, full-text screening, quality assessment, and data extraction. Two authors will independently screen retrieved articles, and a third author authorized to resolve conflicts will handle disagreements. The Joanna Briggs Institute’s (JBI) critical appraisal tools will be used to assess study quality. Appropriate statistical tests will be conducted to quantify the between studies heterogeneity and for the assessment of publication bias. We will check individual study influence analysis and also do subgroup analysis. The STATA version 14.2 will be used for statistical analysis.

Discussion

A high-level adherence to ART is required to achieve adequate viral suppression and improve quality of life. Consequently, the evidence on how adherence to ART differs with knowledge of one’s own HIV status and self-disclosure may help guide interventions aimed at improving adherence to ART.
Wavelet approximation of a function using Chebyshev wavelets
Authors: Nigam H. K. , Mohapatra R. N. , Murari K.
Published: July 2020

In this paper, we estimate the best wavelet approximations of a function f having bounded second derivatives and bounded higher-order derivatives using Chebyshev wavelets of third and fourth kinds.
The significance of case detection ratios for predictions on the outcome of an epidemic - a message from mathematical modelers
Authors: Fuhrmann Jan , Barbarossa Maria Vittoria
Published: July 2020

In attempting to predict the further course of the novel coronavirus disease (COVID-19) pandemic caused by SARS-CoV-2, mathematical models of different types are frequently employed and calibrated to reported case numbers. Among the major challenges in interpreting these data is the uncertainty about the amount of undetected infections, or conversely: the detection ratio. As a result, some models make assumptions about the percentage of detected cases among total infections while others completely neglect undetected cases. Here, we illustrate how model projections about case and fatality numbers vary significantly under varying assumptions on the detection ratio. Uncertainties in model predictions can be significantly reduced by representative testing, both for antibodies and active virus RNA, to uncover past and current infections that have gone undetected thus far.
Aboveground carbon stock is related to land cover and woody species diversity in tropical ecosystems of Eastern Ethiopia
Authors: Sintayehu Dejene W. , Belayneh Anteneh , Dechassa Nigussie
Published: July 2020

Background

Current theories on biodiversity-carbon sequestration relationship describe biodiversity as an important factor influencing carbon storage, either through complementarity effect or by mass ratio effect. So far, the expected form of biodiversity-carbon relationships in tropical ecosystems has not been known with certainty. Therefore, we explored the relationship between aboveground carbon stock and different biodiversity measurement indices (i.e., species richness, species diversity, species evenness, and functional diversity) in different land cover types of Eastern Ethiopia. A total of 48 plots were established using stratified random sampling. Vegetation parameters such as diameter at breast height, diameter at stump height, tree height, and species type were recorded.

Results

We found that the average aboveground carbon stock of the study area is 147.6 ± 17.2 t ha−1 (mean, SE) across land cover types. Species richness, Shannon index, and functional diversity together explained 73.5%, 61.4%, 58.9%, and 52.0% of the variation in aboveground carbon storage in woodland, riparian forest, bushland, and farmland, respectively. Functional diversity was a significant predictor explaining the total aboveground carbon stocks (26.7%) across the land cover types. The effects of biodiversity on aboveground carbon storage were mediated by functional diversity and presence and dominance of species. This shows that both the selection effects and the niche complementarity are important for carbon storage. However, the impact of functional diversity effects (niche complementarity) was higher than that of functional dominance effects (selection effects).

Conclusions

Implementation of protected area-based ecosystem conservation practices in the country seems feasible to mitigate climate change and Reducing Emissions from Deforestation and Forest Degradation (REDD+) programme should emphasize on biodiversity conservation.
The effect of temporary closure and enhanced terminal disinfection using aerosolized hydrogen peroxide of an open-bay intensive care unit on the acquisition of extensively drug-resistant Acinetobacter baumannii
Authors: Moghnieh Rima , Tamim Hani , Jadayel Marwa , Abdallah Dania , Al-Kassem Rasha , Kadiri Hind , Hafez Hani , Al-Hassan Salam , Ajjour Lina , Lakkis Rawad , Jisr Tamima , Samaha Nadia-Lara , Haddad Nicholas
Published: July 2020

Background

At Makassed Hospital’s open-bay intensive care unit (ICU), enhanced terminal disinfection (ETD) using hydrogen peroxide (H2O2) was performed without a predefined schedule in extensively-drug-resistant Acinetobacter baumannii (XDR-AB) outbreaks. In this study, we aimed to check for the value of the temporary closure of the ICU and the use of ETD with aerosolized H2O2 and Ag+ on minimizing the rate of XDR-AB acquisition in patients admitted to the ICU of our facility, which might consequently help us determine the optimal schedule for such procedure in this unit.

Methods

This is a retrospective medical file review of patients admitted to the ICU between January 2016 and May 2018. We divided this period into numerical weeks (NW) after each closure and ETD episode. Risk factors of acquisition (RFA) were determined by comparing the characteristics of patients who acquired XDR-AB to those who didn’t. The proportion of patients residing in each NW was included in the RFA analysis.

Results

Out of 335 patients, 13% acquired XDR-AB. The overall incidence of XDR-AB acquisition was 14.6 cases/1000 patient days. RFA were XDR-AB contact pressure ≥ 3 days [Odds Ratio (OR) = 9.86, 95% Confidence Interval (CI) (3.65–26.64), P < 0.0001)], mechanical ventilation [OR = 4.99, 95%CI (1.76–14.15), P = 0.002)], and having a wound [OR = 3.72, 95%CI (0.99–13.96), P = 0.05)]. Patients who stayed during NW 7,11 and 14 were at risk of acquisition where the odds significantly increased by 6.5, 9.7 and 14.4 folds respectively (P = 0.03,0.01, and 0.01, respectively). We considered NW 7 as the most suitable time for temporary closure of the ICU and ETD with aerosolized H2O2.

Conclusion

Contact pressure, mechanical ventilation, and presence of a wound were RFA of XDR-AB. Temporary closure of the ICU with ETD using aerosolized H2O2 decreased the rate of XDR-AB acquisition, yet this effect fades away with time. The ETD was shown to be most efficiently done when repeated every 7 calendar weeks in our open-bay ICU as part of a prevention bundle.
Gut microbiome signatures of nursing home residents carrying Enterobacteria producing extended-spectrum β-lactamases
Authors: Le Bastard Quentin , Chapelet Guillaume , Birgand Gabriel , Hillmann Benjamin M. , Javaudin François , Hayatgheib Niki , Bourigault Céline , Bemer Pascale , De Decker Laure , Batard Eric , Lepelletier Didier , Montassier Emmanuel
Published: July 2020

Background

The prevalence of extended beta-lactamase producing Enterobacteriaceae (ESBL-E) has been constantly increasing over the last few decades. These microorganisms that have acquired broad antibiotic resistance are now common human pathogens. Changes in the gut microbiome, induced by antibiotics or other drugs, enable expansion of these microorganisms, but the mechanisms are not yet fully understood.

Objectives

The main objective was to identify specific bacteria and functional pathways and genes characterizing the gut microbiome of nursing home residents carrying ESBL-E, using metagenomics.

Subjects and methods

We included 144 residents living in two different nursing homes. All fecal samples were screened for ESBL-E and gut microbiome was characterized using shallow shotgun metagenomic DNA sequencing.

Results

Ten nursing home residents were colonized by ESBL-E, namely Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae species, and were compared to non-carriers. We found that ESBL-E carriers had an alteration in within-sample diversity. Using a bootstrap algorithm, we found that the gut microbiome of ESBL-E carriers was depleted in butyrate-producing species, enriched in succinate-producing species and enriched in pathways involved in intracellular pH homeostasis compared to non-carriers individuals. Several energy metabolism pathways were overrepresented in ESBL-E carriers suggesting a greater ability to metabolize multiple microbiota and mucus layer-derived nutrients.

Conclusions

The gut microbiome of ESBL-E carriers in nursing homes harbors specific taxonomic and functional characteristics, conferring an environment that enables Enterobacteriaceae expansion. Here we describe new functional features associated with ESBL-E carriage that could help us to elucidate the complex interactions leading to colonization persistence in the human gut microbiota.
Movements, habitat utilization, and post-release survival of cobia (Rachycentron canadum) that summer in Virginia waters assessed using pop-up satellite archival tags
Authors: Jensen Douglas R. , Graves John E.
Published: July 2020

Background

Cobia (Rachycentron canadum) is a cosmopolitan marine fish that inhabits tropical, sub-tropical, and temperate marine and estuarine waters and supports a major recreational fishery along the U.S. Atlantic and Gulf of Mexico coasts. Recent changes in U.S. cobia management have sparked controversy and highlighted limitations in our understanding of the species’ biology. This study utilized pop-up satellite archival tags (PSATs) to assess the movements, habitat utilization, and post-release survival of cobia that summer in Virginia waters.

Results

PSATs were deployed on 36 cobia caught in Virginia state waters using standard recreational techniques in August 2016 and August–September 2017. All fish larger than 37-in total length were tagged, and several of these were hooked deeply. No mortalities were inferred for the 20 cobia whose PSATs reported and remained attached for at least 10 days. Premature release of tags was an issue, and only five PSATs remained attached for the full 180-day deployment period. Some fish undertook long seasonal movements, with one individual entering Florida waters well beyond the current stock demarcation boundary. Several fish overwintered in waters offshore of North Carolina near the continental shelf break. Cobia demonstrated a strong affinity for waters ≥ 20 °C, even in the coldest months. They displayed distinct seasonal differences in habitat utilization, spending high proportions of their time near the surface during the summer months and extended periods at depths ≥ 20 m in the winter months.

Conclusions

Cobia are hardy fish with low post-release mortality when handled respectfully. Their migratory patterns are clearly temperature driven, and seasonal changes in habitat utilization means varied vulnerability to fishing gears. Further understanding of cobia migratory patterns, particularly in conjunction with spawning activity, is needed to best manage this species.
Impairment in dynein-mediated nuclear translocation by BICD2 C-terminal truncation leads to neuronal migration defect and human brain malformation
Authors: Tsai Meng-Han , Cheng Haw-Yuan , Nian Fang-Shin , Liu Chen , Chao Nian-Hsin , Chiang Kuo-Liang , Chen Shu-Fang , Tsai Jin-Wu
Published: July 2020

During brain development, the nucleus of migrating neurons follows the centrosome and translocates into the leading process. Defects in these migratory events, which affect neuronal migration, cause lissencephaly and other neurodevelopmental disorders. However, the mechanism of nuclear translocation remains elusive. Using whole exome sequencing (WES), we identified a novel nonsense BICD2 variant p.(Lys775Ter) (K775X) from a lissencephaly patient. Interestingly, most BICD2 missense variants have been associated with human spinal muscular atrophy (SMA) without obvious brain malformations. By in utero electroporation, we showed that BicD2 knockdown in mouse embryos inhibited neuronal migration. Surprisingly, we observed severe blockage of neuronal migration in cells overexpressing K775X but not in those expressing wild-type BicD2 or SMA-associated missense variants. The centrosome of the mutant was, on average, positioned farther away from the nucleus, indicating a failure in nuclear translocation without affecting the centrosome movement. Furthermore, BicD2 localized at the nuclear envelope (NE) through its interaction with NE protein Nesprin-2. K775X variant disrupted this interaction and further interrupted the NE recruitment of BicD2 and dynein. Remarkably, fusion of BicD2-K775X with NE-localizing domain KASH resumed neuronal migration. Our results underscore impaired nuclear translocation during neuronal migration as an important pathomechanism of lissencephaly.
Fyn depletion ameliorates tauP301L-induced neuropathology
Authors: Liu Guanghao , Fiock Kimberly L. , Levites Yona , Golde Todd E. , Hefti Marco M. , Lee Gloria
Published: July 2020

The Src family non-receptor tyrosine kinase Fyn has been implicated in neurodegeneration of Alzheimer’s disease through interaction with amyloid β (Aβ). However, the role of Fyn in the pathogenesis of primary tauopathies such as FTDP-17, where Aβ plaques are absent, is poorly understood. In the current study, we used AAV2/8 vectors to deliver tauP301L to the brains of WT and Fyn KO mice, generating somatic transgenic tauopathy models with the presence or absence of Fyn. Although both genotypes developed tau pathology, Fyn KO developed fewer neurofibrillary tangles on Bielschowsky and Thioflavin S stained sections and showed lower levels of phosphorylated tau. In addition, tauP301L-induced behavior abnormalities and depletion of synaptic proteins were not observed in the Fyn KO model. Our work provides evidence for Fyn being a critical protein in the disease pathogenesis of FTDP-17.
Molecular and clinicopathologic features of gliomas harboring NTRK fusions
Authors: Torre Matthew , Vasudevaraja Varshini , Serrano Jonathan , DeLorenzo Michael , Malinowski Seth , Blandin Anne-Florence , Pages Melanie , Ligon Azra H. , Dong Fei , Meredith David M. , Nasrallah MacLean P. , Horbinski Craig , Dahiya Sonika , Ligon Keith L. , Santi Mariarita , Ramkissoon Shakti H. , Filbin Mariella G. , Snuderl Matija , Alexandrescu Sanda
Published: July 2020

Fusions involving neurotrophic tyrosine receptor kinase (NTRK) genes are detected in ≤2% of gliomas and can promote gliomagenesis. The remarkable therapeutic efficacy of TRK inhibitors, which are among the first Food and Drug Administration-approved targeted therapies for NTRK-fused gliomas, has generated significant clinical interest in characterizing these tumors. In this multi-institutional retrospective study of 42 gliomas with NTRK fusions, next generation DNA sequencing (n = 41), next generation RNA sequencing (n = 1), RNA-sequencing fusion panel (n = 16), methylation profile analysis (n = 18), and histologic evaluation (n = 42) were performed. All infantile NTRK-fused gliomas (n = 7) had high-grade histology and, with one exception, no other significant genetic alterations. Pediatric NTRK-fused gliomas (n = 13) typically involved NTRK2, ranged from low- to high-histologic grade, and demonstrated histologic overlap with desmoplastic infantile ganglioglioma, pilocytic astrocytoma, ganglioglioma, and glioblastoma, among other entities, but they rarely matched with high confidence to known methylation class families or with each other; alterations involving ATRX, PTEN, and CDKN2A/2B were present in a subset of cases. Adult NTRK-fused gliomas (n = 22) typically involved NTRK1 and had predominantly high-grade histology; genetic alterations involving IDH1, ATRX, TP53, PTEN, TERT promoter, RB1, CDKN2A/2B, NF1, and polysomy 7 were common. Unsupervised principal component analysis of methylation profiles demonstrated no obvious grouping by histologic grade, NTRK gene involved, or age group. KEGG pathway analysis detected methylation differences in genes involved in PI3K/AKT, MAPK, and other pathways. In summary, the study highlights the clinical, histologic, and molecular heterogeneity of NTRK-fused gliomas, particularly when stratified by age group.
Hydrogen sulfide removal from geothermal fluids by Fe(III)-based additives
Authors: Regenspurg S. , Iannotta J. , Feldbusch E. , Zimmermann F. J. , Eichinger F.
Published: July 2020

A new method to remove hydrogen sulfide from geothermal fluids during well operation was tested in situ at a geothermal site in Vienna (Austria). For this purpose, ferric iron was added either as granulated iron hydroxide or as FeCl3 solution into a reaction vessel containing the thermal water directly removed from the wells. From the container, the water would be pumped through a particle filter. Physicochemical parameters as well as sulfide were measured constantly over time before and after the filter. It was found that the sulfide was fully removed from the water by both iron additives. While the addition of FeCl3 led first to the formation of black iron(II) sulfide (FeS), which subsequently oxidized in presence of oxygen to Fe(III) hydroxide, no visible change of the granulated iron hydroxide was observed. The reaction time was longer when using the Fe(III) hydroxide additive as compared to the FeCl3 (completed in less than 20 min) but could be enhanced by increasing the amount of added particles. In all experiments the pH was constantly rising during the reaction from about 6.3 to 7.5, which was explained by loss of protons due to purging out of the gaseous H2S. The redox value, which was measured over time, remained rather constant after addition of granulated iron hydroxide (about −350 mV), but strongly increased from −350 mV to −50 mV after adding the FeCl3 suggesting a strong electron-consuming reaction. This can be explained by a two-step reaction: first, the Fe(III) was reduced to Fe(II) by oxidation of either sulfide or thiosulfate to sulfate. Afterward, the Fe(II) oxidized again by dissolved oxygen forming orange Fe(III) hydroxides. The application of the investigated method during operation of geothermal wells could prevent H2S-induced corrosion and would eliminate the toxic effects of this gas.
Homozygous LMNA p.R582H pathogenic variant reveals increasing effect on the severity of fat loss in lipodystrophy
Authors: Soyaltin Utku Erdem , Simsir Ilgin Yildirim , Akinci Baris , Altay Canan , Adiyaman Suleyman Cem , Lee Kristen , Onay Huseyin , Oral Elif Arioglu
Published: July 2020

Background

Classical heterozygous pathogenic variants of the lamin A/C (LMNA) gene cause autosomal dominant familial partial lipodystrophy type 2 (FPLD2). However, recent reports indicate phenotypic heterogeneity among carriers of LMNA pathogenic variants, and a few patients have been associated with generalized fat loss.

Case presentation

Here, we report a patient with a lamin A specific pathogenic variant in exon 11, denoted LMNA (c.1745G > A; p.R582H), present in the homozygous state. Fat distribution was compared radiographically to an unrelated heterozygote LMNA p.R582H patient from another pedigree, a healthy female control, a series of adult female subjects with congenital generalized lipodystrophy type 1 (CGL1, n = 9), and typical FPLD2 (n = 8). The whole-body MRI of the index case confirmed near-total loss of subcutaneous adipose tissue with well-preserved fat in the retroorbital area, palms and soles, mons pubis, and external genital region. This pattern resembled the fat loss pattern observed in CGL1 with only one difference: strikingly more fat was observed around mons pubis and the genital region. Also, the p.R582H LMNA variant in homozygous fashion was associated with lower leptin level and earlier onset of metabolic abnormalities compared to heterozygous p.R582H variant and typical FPLD2 cases. On the other hand, the heterozygous LMNA p.R582H variant was associated with partial fat loss which was similar to typical FPLD2 but less severe than the patients with the hot-spot variants at position 482.

Conclusions

Our observations and radiological comparisons demonstrate an additive effect of LMNA pathogenic variants on the severity of fat loss and add to the body of evidence that there may be complex genotype-phenotype relationships in this interesting disease known as FPLD2. Although the pathological basis for fat loss is not well understood in patients harboring pathogenic variants in the LMNA gene, our observation suggests that genetic factors modulate the extent of fat loss in LMNA associated lipodystrophy.
Psychosocial distress, clinical variables and self-management activities associated with type 2 diabetes: a study in Ghana
Authors: Amankwah-Poku Margaret , Amoah Albert G. B. , Sefa-Dedeh Araba , Akpalu Josephine
Published: July 2020

Aim

Psychosocial distress can act as a barrier to diabetes self-care management and thus compromise diabetes control. Yet in Ghana, healthcare centres mainly focus on the medical aspect of diabetes to the neglect of psychosocial care. This study determined the relationship amongst psychosocial distress, clinical variables, and self-management activities associated with type 2 diabetes management.

Method

Questionnaires were administered to 162 patients from four hospitals in Accra, Ghana, to assess psychosocial distress (e.g. diabetes distress), clinical variables (e.g. glycaemic control), and self- management activities (e.g. medication intake) related to diabetes. In assessing diabetes distress, the use of the PAID allowed evaluation of broader range of emotional concerns (diabetes-related emotional distress), while the DDS allowed evaluation of factors more closely related to diabetes self-management (diabetes distress).

Results

Diabetes-related emotional distress, diabetes distress and depressive symptoms were reciprocally positively correlated, while non-supportive family behaviour correlated negatively with these psychological variables. Diabetes-related emotional distress correlated positively with systolic and diastolic blood pressure, and correlated negatively with exercise regimen. On the other hand, diabetes distress correlated negatively with dietary and exercise regimen and correlated positively with glycaemic levels, while depressive symptoms correlated positively with glycaemic levels, diabetes complication and systolic blood pressure. Contrary to the literature, non-supportive family behaviour correlated positively with diet, exercise and medication regimen.

Conclusion

The positive association of psychological variables with glycaemic levels and blood pressure levels, and the positive association of non-supportive family behaviour with self-management activities suggests the need for psychosocial care to be incorporate in the management of type 2 diabetes in Ghana. Patients can be screened for diabetes-related distress and symptoms of depression and provided psychosocial care where necessary.
Understanding fully online teaching in vocational education
Authors: Cox Deniese , Prestridge Sarah
Published: July 2020

Literature has previously reported that student-centred practices are the mark of good pedagogy in online education. In contrast, the competency-based nature of vocational education in Australia has been understood to encourage teacher-centred pedagogy. The likely tensions between these two teaching contexts are not yet understood, and little is yet known about the pedagogy of fully online vocational education teachers. To begin understanding pedagogy in this context, a wide-ranging digital survey was implemented. Findings revealed that online vocation education teachers conceived good online pedagogy as student-centred, yet student-student learning opportunities were rated lower than teacher-student practices. Notably, enacted practice was consistently more teacher-centred than teachers’ ideal, and factors within the teaching context were perceived by teachers as a limitation. They reported their workload to be dominated by marking and administration ahead of student-centred practices such as building rapport. This work is of interest to researchers and institutions navigating the continued expansion of online education and the ongoing demand for effective student-centred practice.
Evaluating the effectiveness of a preservice teacher technology training module incorporating SQD strategies
Authors: Hsu Yu-Yin , Lin Chin-Hsi
Published: July 2020

Preparation to use information and communication technology (ICT) is an important component of preservice language teachers’ training, and various existing teacher training models propose a range of strategies for increasing their technology knowledge and technology adoption rates. However, the relative effectiveness of these strategies remains unclear. Based on Tondeur et al.’s (2012) Synthesis of Qualitative Data model, which delineates the six main teacher preparation strategies (i.e., role modeling, reflection, instructional design, collaboration, authentic experience, and continuous feedback), the present study designed a 4 week training module for preservice language teachers and examined how these training strategies affected 63 participants’ perceived technology knowledge and attitudes toward technology adoption. Among the six training strategies, reflection and instructional design had the highest positive impacts on these preservice teachers’ self-reported knowledge about and attitudes towards using ICT. As well as revealing the relative impacts of each training strategy, the results indicate that our designed training module has considerable potential for application to teacher training in other subjects.
Phenotypic characterization of the Egyptian isolates “extensively drug-resistant Pseudomonas aeruginosa” and detection of their metallo-β-lactamases encoding genes
Authors: Basha Amr Mohamad , El-Sherbiny Gamal M. , Mabrouk Mona I.
Published: July 2020

Background

Carbapenem antibiotics consider the primary treatment choice for serious Pseudomonas aeruginosa infection. Hence, the evolution of carbapenem resistance mediated by acquiring genes encoding class b enzymes is of global concern. The purpose of this article research is to explore the prevalence, drug resistance profiles, and metallo-β-lactamases (MβLs) production in extensively drug-resistant carbapenem-resistant P. aeruginosa (XDR-CRPA).

Methods

P. aeruginosa isolates were collected and identified according to conventional methods. Antibiotic resistance patterns were determined by single disk diffusion. Minimum inhibitory concentrations (MICs) of (imipenem, meropenem, ceftazidime, piperacillin/tazobactam, levofloxacin, and gentamicin) were determined for CRPA. A subset of the isolates collection consisting of the XDR-CRPA with the highest MICs to imipenem and meropenem were selected for the phenotypic screening of carbapenemases and MβLs production capability using the modified carbapenem inactivation (mCIM) and imipenem-EDTA combined disk (MβL-CD) methods, respectively. Then, molecular analysis, including identification by the specific primer of 16S rRNA and detection of MβL genes using polymerase chain reaction (PCR) was performed to the XDR selected isolates.

Results

Among 100 P. aeruginosa isolated throughout this period, 59% exhibited reduced susceptibility rates to carbapenems. A total of 20.3% and 57% of CRPA isolates were MDR and XDR, respectively. MIC values of the CRPA revealed that these isolates exhibited high MIC50 and MIC90 to the six selected antibiotics. The findings of the (mCIM) assay displayed identical concordance results with the MβL-CD. Molecular investigation technique assured that 10 (90.9%) and 2 (18.1%) of the 11 XDR selected isolates are positive for blaNDM-1 and blaVIM-1 genes, respectively. Polymyxin B and colistin followed by aztreonam were the most effective antibiotics used for curing infections caused by XDR Pseudomonas aeruginosa.

Conclusion

The prevalence of high XDR-CRPA in our study is a critical problem. Our present study found that the blaNDM-1 was present at a significant frequency among the selected XDR isolates, highlighting the need for establishing strict antimicrobial policies to avoid the prompt spread of these isolates.
A case of spastic paraplegia type 11 with a variation in the SPG11 gene
Authors: Elmas Muhsin , Gogus Basak , Değirmenci Banu , Solak Mustafa , Gleeson J. G.
Published: July 2020

Background

Spastic paraplegia 11 (SPG11) is defined as progressive spasticity and weakness of the lower limbs and also associated with mild intellectual disability with learning difficulties in childhood and/or progressive cognitive retardation, peripheral neuropathy, pseudobulbar symptoms, and increased reflexes in the upper limbs. We describe the clinical, laboratory, and radiological presentation of SPG11 through a report of a case and compare with previously reported SPG11 cases in the literature.

Case presentation

This case presents a homozygous variant in the SPG11 gene (NM_025137.4): c.1699C>T;p.(Gln567*).

Conclusion

The diagnosis was made based on molecular findings, thinning of corpus callosum (TCC) and in most cases, periventricular white matter abnormalities are detected in brain MRI. Therefore, the clinical and radiological findings are supporting the diagnosis. However, it should not be forgotten that TCC is not peculiar to SPG11.
Preparation, characterization and evaluation of aspirin: benzoic acid cocrystals with enhanced pharmaceutical properties
Authors: Dutt Braham , Choudhary Manjusha , Budhwar Vikas
Published: July 2020

Background

The cocrystallization process in pharmaceuticals has gained widespread attention as a recent method of modifying physicochemical properties without altering the pharmacological characteristics of drugs. Cocrystallization provides a couple of benefits like it can be employed for a large number of APIs (acidic, basic, ionizable, or non-ionizable), and secondly, the availability of a large number of potential coformers increases the possibility of the cocrystals (CCs) that can be synthesized for an API. The main objective of this study was to investigate the effects of cocrystallization on drugs having poor aqueous solubility.

Results

Aspirin (AN) and benzoic acid (BZ) were cocrystallized by using the solvent evaporation technique. CSD (Cambridge Structure Database) software and ∆pKa value method were used for the selection of the drug and coformer and for prediction of CC formation. The analysis of CCs was performed using DSC (differential scanning calorimetry), FT-IR (Fourier transformation infra-red spectroscopy) and XRD (X-ray diffraction) techniques. In vivo anti-inflammatory studies were conducted on 24 Wistar rats divided into four groups.

Conclusions

Here, in this study, in vitro dissolution studies revealed an improved solubility profile of CCs compared to pure drug and marketed formulation viz. 87%, 31% and 60% respectively. The in vivo anti-inflammatory studies exhibited improved anti-inflammatory activity compared to pure drug. So, on the basis of outcomes of this study, we concluded that cocrystallization process have a direct impact on the improvement of physicochemical characteristics of APIs having issues like solubility or stability without any modification and alteration of their pharmacological actions.
Temperature control of shoot growth and floral initiation in apple (
Malus
×
domestica
Borkh.)
Authors: Heide Ola M. , Rivero Rodmar , Sønsteby Anita
Published: July 2020

Background

The environmental control of flowering in apple (
Malus 
×
 domestica
Borkh.) has remained an enigma for generations.

Methods

We studied growth and flowering effects of temperature under daylight phytotron conditions in 3-year old ‘Summerred’ and ‘Discovery’ apple trees. Flowering was assessed by dissection of buds on spurs and extension shoots at termination of treatments and flowering performance in the subsequent spring.

Results

Exposure to constant temperatures ranging from 12 to 27 °C for 12 weeks yielded a hyperbolic response curve with optimum at 18–21 °C and little or no flowering at 12 and 27 °C. A drop from high to low temperature after 6 weeks caused growth cessation and initiation of flowering, whereas the reverse shift had the opposite effect. Shoot growth and leaf accumulation increased with increasing temperature, but under flower-inducing conditions, both levelled off and ceased towards the end of the treatment period.

Conclusions

The results are discussed in relation to the extensive physiological and genetic literature on the subject. We interpret the results as two separate effects of temperature on flowering in apple. At 12 °C flowering seems to be limited by low temperature depression of growth and leaf production, while at 27 °C, flowering is blocked by inhibition of the floral initiation itself. Intermediate temperatures of 18–21 °C, on the other hand, seem to satisfy the requirements for both processes. These opposite effects of temperature have apparently confounded the understanding of the environmental control of flowering in apple.
Finding a Fit: Biological Science Doctoral Students’ Selection of a Principal Investigator and Research Laboratory
Authors: Maher Michelle A. , Wofford Annie M. , Roksa Josipa , Feldon David F.
Published: July 2020

This study explored how biological science doctoral students made decisions about fitting with a principal investigator (PI) and within a research lab. Results illuminated a complex array of factors students considered in selecting a PI, including PI mentoring style and professional stability. Peers and research projects played an important role in students’ lab selection.
Prevention and Management of Chemotherapy-Induced Peripheral Neuropathy in Survivors of Adult Cancers: ASCO Guideline Update
Authors: Loprinzi Charles L. , Lacchetti Christina , Bleeker Jonathan , Cavaletti Guido , Chauhan Cynthia , Hertz Daniel L. , Kelley Mark R. , Lavino Antoinette , Lustberg Maryam B. , Paice Judith A. , Schneider Bryan P. , Lavoie Smith Ellen M. , Smith Mary Lou , Smith Thomas J. , Wagner-Johnston Nina , Hershman Dawn L.
Published: July 2020

PURPOSE

To update the ASCO guideline on the recommended prevention and treatment approaches in the management of chemotherapy-induced peripheral neuropathy (CIPN) in adult cancer survivors.

METHODS

An Expert Panel conducted targeted systematic literature reviews to identify new studies.

RESULTS

The search strategy identified 257 new references, which led to a full-text review of 87 manuscripts. A total of 3 systematic reviews, 2 with meta-analyses, and 28 primary trials for prevention of CIPN in addition to 14 primary trials related to treatment of established CIPN, are included in this update.

RECOMMENDATIONS

The identified data reconfirmed that no agents are recommended for the prevention of CIPN. The use of acetyl-l-carnitine for the prevention of CIPN in patients with cancer should be discouraged. Furthermore, clinicians should assess the appropriateness of dose delaying, dose reduction, substitutions, or stopping chemotherapy in patients who develop intolerable neuropathy and/or functional impairment. Duloxetine is the only agent that has appropriate evidence to support its use for patients with established painful CIPN. Nonetheless, the amount of benefit from duloxetine is limited. Additional information is available at www.asco.org/survivorship-guidelines .