bluuebunny/crossref_metadata_2025 huggingface dataset:
Steam Refining with Subsequent Alkaline Lignin Extraction as an Alternative Pretreatment Method to Enhance the Enzymatic Digestibility of Corn Stover
Authors: Krafft Malte Jörn , Bendler Marie , Schreiber Andreas , Saake Bodo
Published: June 2020

Agricultural residues are promising and abundant feedstocks for the production of monomeric carbohydrates, which can be gained after pretreatment and enzymatic hydrolysis. These monomeric carbohydrates can be fermented to platform chemicals, like ethanol or succinic acid. Due to its high availability, corn stover is a feedstock of special interest in such considerations. The natural recalcitrance of lignocellulosic material against degradation necessitates a pretreatment before the enzymatic hydrolysis. In the present study, a novel combination of steam refining and alkaline lignin extraction was tested as a pretreatment process for corn stover. This combination combines the enhancement of the enzymatic hydrolysis and steam refining lignin can be gained for further utilization. Afterward, the obtained yields after enzymatic hydrolysis were compared with those after steam refining without alkaline extraction. After steam refining at temperatures between 160 °C and 210 °C and subsequent enzymatic hydrolysis with Cellic® CTec2, it was possible to enhance the digestibility of corn stover and to achieve 65.4% of the available carbohydrates at the lowest up to 89% at the highest conditions as monomers after enzymatic hydrolysis. Furthermore, the enzymatic degradation could be optimized with a subsequent alkaline lignin extraction, especially at low severities under three. After this combined pretreatment, it was possible to enhance the enzymatic digestibility and to achieve up to 106.4% of the available carbohydrates at the lowest conditions and up to 102.2% at the highest temperature as monomers after following enzymatic hydrolysis, compared to analytical acid hydrolysis. Regarding the utilization of the arising lignin after extraction, the lignin was characterized with regard to the molar mass and carbohydrate impurities. In this context, it was found that higher amounts and higher purities of lignin can be attained after pretreatment at severities higher than four.
Arbuscular Mycorrhizal Fungi as Potential Agents in Ameliorating Heavy Metal Stress in Plants
Authors: Dhalaria Rajni , Kumar Dinesh , Kumar Harsh , Nepovimova Eugenie , Kuča Kamil , Torequl Islam Muhammad , Verma Rachna
Published: June 2020

Heavy metal accumulation in plants is a severe environmental problem, rising at an expeditious rate. Heavy metals such as cadmium, arsenic, mercury and lead are known environmental pollutants that exert noxious effects on the morpho-physiological and biological attributes of a plant. Due to their mobile nature, they have become an extended part of the food chain and affect human health. Arbuscular mycorrhizal fungi ameliorate metal toxicity as they intensify the plant’s ability to tolerate metal stress. Mycorrhizal fungi have vesicles, which are analogous to fungal vacuoles and accumulate massive amount of heavy metals in them. With the help of a pervasive hyphal network, arbuscular mycorrhizal fungi help in the uptake of water and nutrients, thereby abating the use of chemical fertilizers on the plants. They also promote resistance parameters in the plants, secrete a glycoprotein named glomalin that reduces the metal uptake in plants by forming glycoprotein–metal complexes, and improve the quality of the soil. They also assist plants in phytoremediation by increasing the absorptive area, increase the antioxidant response, chelate heavy metals and stimulate genes for protein synthesis that reduce the damage caused by free radicals. The current manuscript focuses on the uptake of heavy metals, accumulation, and arbuscular mycorrhizal impact in ameliorating heavy metal stress in plants.
Fungal Endophytes as Biocontrol Agents against the Main Soil-Borne Diseases of Melon and Watermelon in Spain
Authors: González Vicente , Armijos Eugenia , Garcés-Claver Ana
Published: June 2020

Watermelon and melon crops are affected by some important soil-borne fungal diseases like carbonaceous rot (Macrophomina phaseolina), collapse (Monosporascus cannonballus), or the most important pathology at an economic level, the Fusarium wilt (Fusarium oxysporum f. sp. niveum, F. oxysporum f. sp. melonis, F. solani f. sp. cucurbitae, Neocosmospora falciformis, and N. keratoplastica). The methods commonly used for their control are often ineffective, thus new approaches, as the use of biological control agents, are constantly being sought. This work aimed to isolate, identify, and test endophytic fungi for their antagonistic properties against the three mentioned diseases. For this, about 350 endophytic fungal strains were isolated from asymptomatic watermelon plants. Among these, 7 fungal species were selected to evaluate their antagonistic potential against 14 pathogens. Dual culture assays allowed to select two Trichoderma strains according to the high inhibition rates observed (up to 93%), that were further employed in melon and watermelon plants, showing that some of the pathogens were controlled in terms of disease incidence, exhibiting a decrease up to 67% for T. lentiforme. In addition, three concentrations of Epicoccum purpurascens extract was selected to evaluate the germicide effect, obtaining significant differences in the growth of the pathogens depending on fermentation parameters.
Challenges and Prospects of Biogas from Energy Cane as Supplement to Bioethanol Production
Authors: Hoffstadt Kevin , Pohen Gino D. , Dicke Max D. , Paulsen Svea , Krafft Simone , Zang Joachim W. , da Fonseca-Zang Warde A. , Leite Athaydes , Kuperjans Isabel
Published: June 2020

Innovative breeds of sugar cane yield up to 2.5 times as much organic matter as conventional breeds, resulting in a great potential for biogas production. The use of biogas production as a complementary solution to conventional and second-generation ethanol production in Brazil may increase the energy produced per hectare in the sugarcane sector. Herein, it was demonstrated that through ensiling, energy cane can be conserved for six months; the stored cane can then be fed into a continuous biogas process. This approach is necessary to achieve year-round biogas production at an industrial scale. Batch tests revealed specific biogas potentials between 400 and 600 LN/kgVS for both the ensiled and non-ensiled energy cane, and the specific biogas potential of a continuous biogas process fed with ensiled energy cane was in the same range. Peak biogas losses through ensiling of up to 27% after six months were observed. Finally, compared with second-generation ethanol production using energy cane, the results indicated that biogas production from energy cane may lead to higher energy yields per hectare, with an average energy yield of up to 162 MWh/ha. Finally, the Farm2CBG concept is introduced, showing an approach for decentralized biogas production.
Dietary Supplementation of Postbiotics Mitigates Adverse Impacts of Heat Stress on Antioxidant Enzyme Activity, Total Antioxidant, Lipid Peroxidation, Physiological Stress Indicators, Lipid Profile and Meat Quality in Broilers
Authors: Humam Ali Merzza , Loh Teck Chwen , Foo Hooi Ling , Izuddin Wan Ibrahim , Awad Elmutaz Atta , Idrus Zulkifli , Samsudin Anjas Asmara , Mustapha Noordin Mohamed
Published: June 2020

The purpose of this work was to evaluate the impacts of feeding different postbiotics on oxidative stress markers, physiological stress indicators, lipid profile and meat quality in heat-stressed broilers. A total of 252 male Cobb 500 (22-day-old) were fed with 1 of 6 diets: A basal diet without any supplementation as negative control (NC); basal diet + 0.02% oxytetracycline served as positive control (PC); basal diet + 0.02% ascorbic acid (AA); or the basal diet diet + 0.3% of RI11, RS5 or UL4 postbiotics. Postbiotics supplementation, especially RI11 increased plasma activity of total-antioxidant capacity (T-AOC), catalase (CAT) and glutathione (GSH), and decreased alpha-1-acid-glycoprotein (α1-AGP) and ceruloplasmin (CPN) compared to NC and PC groups. Meat malondialdehyde (MDA) was lower in the postbiotic groups than the NC, PC and AA groups. Plasma corticosterone, heat shock protein70 (HSP70) and high density lipoprotein (HDL) were not affected by dietary treatments. Postbiotics decreased plasma cholesterol concentration compared to other groups, and plasma triglyceride and very low density lipoprotein (VLDL) compared to the NC group. Postbiotics increased breast meat pH, and decreased shear force and lightness (L*) compared to NC and PC groups. The drip loss, cooking loss and yellowness (b*) were lower in postbiotics groups compared to other groups. In conclusion, postbiotics particularly RI11 could be used as an alternative to antibiotics and natural sources of antioxidants for heat-stressed broilers.
A New Information System for the Management of Non-Epidemic Veterinary Emergencies
Authors: Possenti Luigi , Savini Lara , Conte Annamaria , D'Alterio Nicola , Danzetta Maria Luisa , Di Lorenzo Alessio , Nardoia Maria , Migliaccio Paolo , Tora Susanna , Dalla Villa Paolo
Published: June 2020

The Italian National Veterinary Services, public health professionals, and policy makers are asked to participate at different levels in the decision-making process for the management of non-epidemic emergencies. A decision support system offering the different administrative and operational emergency management levels with a spatial and decisional tool to be used in the case of natural disasters is still missing at the national level. Within this context, the Italian General Directorate for Animal Health of the Ministry of Health funded a research project for the implementation of a new Veterinary Information System for Non-Epidemic Emergencies (SIVENE), an innovative real-time decision support tool for emergency response in a disaster management scenario. SIVENE was developed according to a multi-layer architecture with four integrated components: the database layer, which was implemented by an RDBMS Oracle 11 g; the ReST service layer, which was created using J2EE, Spring, and MyBatis technologies; the web application (business framework and user interface), which was developed in Angular4 framework using TypeScript language; and the web Geographic Information Systems (GIS), which was realized through the implementation of a geodatabase in Oracle RDBMS 11 g. This system allows us to build up and dynamically create a set of dedicated checklists to be used in the field when gathering the information needed for the management of non-epidemic emergencies; employ the application on mobile devices, such as tablets and smartphones; and use the web GIS to manage and visualize data of veterinary interest and territorial maps of risk and damage.
Evaluation of Sugarcane-Derived Polyphenols on the Pre-Weaning and Post-Weaning Growth of Gilt Progeny
Authors: Wijesiriwardana Udani A. , Pluske John R. , Craig Jessica R. , Cottrell Jeremy J. , Dunshea Frank R.
Published: June 2020

Gilt progeny (GP) exhibit poorer growth compared with sow progeny (SP), particularly in the pre-weaning and post-weaning period. Late gestation/lactation sow diets and weaner diets were supplemented with 0.5% Polygain (POL), a sugarcane extract rich in polyphenols, to collectively improve GP growth in these periods. Gilts (n = 60) and sows (n = 68, parities 2 and 3) were fed a control or POL diet. Weaned GP (n = 79) and SP (n = 92) born to these dams were also fed either a CON or POL diet. Gilts litters weighed less than sow litters at birth and 21 days (p < 0.001 for both) and were not improved by POL (p = 0.80 and 0.54, respectively). GP were lighter than SP at day 7 and day 14 post-weaning (p < 0.001 for both) and were not improved by the POL diet at these timepoints (p = 0.61 and 0.97, respectively). Plasma interleukin-1β (IL-1β) was increased at weaning despite POL supplementation (p = 0.022) and GP had reduced IL-1β overall (p = 0.021). Overall, POL was unable to improve growth in GP and the attenuated immune response seen in GP could be contributing to their poor growth performance.
Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes
Authors: Slama Petr , Kabourkova Eliska , Sladek Zbysek , Zavadilova Terezie , Kratochvilova Lucie , Kharkevich Kristina , Roychoudhury Shubhadeep , Pavlik Ales , Roztocilova Andrea , Uhrincat Michal , Tancin Vladimir , Kimura Kazuhiro , Konecny Roman , Kiku Yoshio , Watanabe Atsushi , Kwak Jong-Young , Zouharova Monika
Published: June 2020

The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of Escherichia coli or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 h after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-h following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors.
Evidence for Citation Networks in Studies of Free-Roaming Cats: A Case Study Using Literature on Trap–Neuter–Return (TNR)
Authors: Calver Michael C. , Fleming Patricia A.
Published: June 2020

Trap–Neuter–Return and its variants (hereafter TNR) aims to control unowned cat populations. Papers on this topic form a useful case study of how how an area of literature grows, papers become influential, and citation networks form, influencing future study as well as public perceptions of the science. We analysed 145 TNR studies published 2002–2019. Common topics, identified by frequently used language, were population control, interactions with wildlife, disease transmission (including implications for pets, wildlife and humans), free-roaming cats, and feral and domestic cat management. One or more papers on each of these topics was judged influential because of high citations overall, high average citations/year, or frequent mentions in social media. Open Access papers were more influential in social media, raising greater public awareness than studies published in journals that were less accessible. While divergent views exist on a range of topics, the network analysis of the TNR literature indicated potential for forming self-reinforcing groups of authors. While it is encouraging that diverse views are expressed, there is a risk of reduced dialogue interactions between groups, potentially constraining dialogue to refine arguments, share information, or plan research. Journal editors could encourage communication by choosing reviewers from different camps to assess manuscripts and by asking authors to acknowledge alternative views.
Human-Leopard Conflict: An Emerging Issue of North China Leopard Conservation in Tieqiaoshan Provincial Nature Reserve in Shanxi Province, China
Authors: Consolee Kahindo Tulizo , Gao Chunyv , Vitekere Kasereka , Li Chunshi , Yan Hua , Jiang Guangshun
Published: June 2020

Livestock depredation by large carnivores is a conventional human–wildlife conflict, both at the local and regional level. Many species of wildlife have become endangered because of this conflict. In this study, an investigation of livestock depredation was conducted for the north China leopard in and around Tieqiaoshan Provincial Nature Reserve in Shanxi Province between 2015 and 2018. Data were obtained from League Cat Forest Department records. Additionally, standardized, structured, and semi-structured questionnaires were used to collect data with the help of reserve field staff. The results show that there was a significant difference (p = 0.015) in livestock depredation in various seasons of the year; the highest depredation was recorded in spring, followed by summer. A significant difference (p = 0.02) was observed between cattle and other livestock species, showing that more cattle were killed by the north China leopard. Most of the livestock depredation occurred during late morning and evening, likely because the leopards are crepuscular. Residents in and around the reserve suffered a high economic loss, ranging between RMB 5000 and 10,000 (USD 706.76–1413.53) per year in terms of the estimated market price of the killed livestock. The attitudes of residents towards the north China leopard vary according to the economic activities of the locals, with about 76% of the livestock keepers reporting that the leopard is “very dangerous” and 8% of the arable farmers in and around the reserve indicating that leopard is “very dangerous.” We recommend that a system with local participants would ensure more effective management of human-north China leopard conflict, as it would allow local communities to take greater responsibility.
Validation of Contact Mats to Monitor Dairy Cow Contact with Stall Partitions
Authors: Freinberg Caroline , Zambelis Athena , Vasseur Elsa
Published: June 2020

In indoor housing systems, repetitive contact with the stall partitions may reflect issues between the housing environment and the cow, by reducing the quality of rest and hindering ease of movement. The objective of this study was to validate the ability of a contact mat (CM) system to monitor cow contact with stall dividers and neck rail when compared to video observation. Eleven lactating cows were monitored using video recording and with the CM system for 4 h/d for 4 consecutive days. CM were affixed to the stall dividers and neck rail to record the frequency of cow contact per second. Two observers recorded the frequency of cow contact against the stall partitions per second using three criteria: cow contact with the stall dividers or neck rail regardless of CM contact (Total Rail Contact), cow contact with the CM regardless of placement or force (Total CM Contact), and cow contact with the CM through proper placement and force (Effective CM Contact). The correlation strength used to measure agreement between video observation and CM system for cow ranking based on degree of contact varied from moderate to very high (dividers: Total Rail Contact: rs = 0.68, Total CM Contact rs = 0.90, Effective CM Contact rs = 0.90; neck rail: Total Rail Contact: rs = 0.71, Total CM Contact rs = 0.66, Effective CM Contact rs = 0.58). CM can be used to accurately rank cows based on their frequency of contact with the stall dividers. This can be used to identify individual cows that need intervention for stall comfort risk factors, or to assess which indoor housing environments offer fewer constraints and better movement opportunities.
Physiological Stress Reactions in Red Deer Induced by Hunting Activities
Authors: Vilela Sofia , Alves da Silva António , Palme Rupert , Ruckstuhl Kathreen E. , Sousa José Paulo , Alves Joana
Published: June 2020

Hunting activity is usually seen as a factor capable of causing an intense stress response in wildlife that may lead to short but also long-term stress. In the Lousã Mountain, Portugal, the population of red deer (Cervus elaphus) is the target of intensive seasonal hunting. We collected and measured cortisol (and its metabolites) in three tissues types (blood, feces and hair) from red deer hunted during two hunting seasons to evaluate the stress levels at different time windows. We also assessed the immunological and physical condition of the animals. We predicted that the hunting activity would act as a stressor inducing increased short and long-term stress levels in the population. Results showed an increase in hair cortisol levels during the months of harvesting. Surprisingly, the tendency for plasma cortisol levels was to decrease during the hunting season, which could be interpreted as habituation to hunting activity, or due to the hunting duration. Contrary to our predictions, fecal cortisol metabolites did not show any clear patterns across the months. Overall, our results suggest an influence of hunting activities on the physiological stress in red deer. In addition, hair seems to be useful to measure physiological stress, although more studies are required to fully understand its suitability as an indicator of long-term stress. Methodologically, our approach highlights the importance of simultaneously using different methods to assess short and long-term effects in studies on physiological stress reactions.
Genetic Diversity and Signatures of Selection in a Native Italian Horse Breed Based on SNP Data
Authors: Ablondi Michela , Dadousis Christos , Vasini Matteo , Eriksson Susanne , Mikko Sofia , Sabbioni Alberto
Published: June 2020

Horses are nowadays mainly used for sport and leisure activities, and several local breeds, traditionally used in agriculture, have been exposed to a dramatic loss in population size and genetic diversity. The loss of genetic diversity negatively impacts individual fitness and reduces the potential long-term survivability of a breed. Recent advances in molecular biology and bioinformatics have allowed researchers to explore biodiversity one step further. This study aimed to evaluate the loss of genetic variability and identify genomic regions under selection pressure in the Bardigiano breed based on GGP Equine70k SNP data. The effective population size based on Linkage Disequilibrium (Ne) was equal to 39 horses, and it showed a decline over time. The average inbreeding based on runs of homozygosity (ROH) was equal to 0.17 (SD = 0.03). The majority of the ROH were relatively short (91% were ≤2 Mbp long), highlighting the occurrence of older inbreeding, rather than a more recent occurrence. A total of eight ROH islands, shared among more than 70% of the Bardigiano horses, were found. Four of them mapped to known quantitative trait loci related to morphological traits (e.g., body size and coat color) and disease susceptibility. This study provided the first genome-wide scan of genetic diversity and selection signatures in an Italian native horse breed.
Impact of a Diagnosis-Centered Antibiotic Stewardship on Incident Clostridioides difficile Infections in Older Inpatients: An Observational Study
Authors: Putot Alain , Astruc Karine , Barben Jeremy , Mihai Anca Maria , Nuss Valentine , Bador Julien , Putot Sophie , Dipanda Mélanie , Laborde Caroline , Vovelle Jeremie , Da Silva Sofia , Mazen Emmanuel , Aho Glélé Ludwig Serge , Manckoundia Patrick
Published: June 2020

In 2015, a major increase in incident hospital-onset Clostridioides difficile infections (HO-CDI) in a geriatric university hospital led to the implementation of a diagnosis-centered antibiotic stewardship program (ASP). We aimed to evaluate the impact of the ASP on antibiotic consumption and on HO-CDI incidence. The intervention was the arrival of a full-time infectiologist in the acute geriatric unit in May 2015, followed by the implementation of new diagnostic procedures for infections associated with an antibiotic withdrawal policy. Between 2015 and 2018, the ASP was associated with a major reduction in diagnoses for inpatients (23% to 13% for pneumonia, 24% to 13% for urinary tract infection), while median hospital stays and mortality rates remained stable. The reduction in diagnosed bacterial infections was associated with a 45% decrease in antibiotic consumption in the acute geriatric unit. HO-CDI incidence also decreased dramatically from 1.4‰ bed-days to 0.8‰ bed-days in the geriatric rehabilitation unit. The ASP focused on reducing the overdiagnosis of bacterial infections in the acute geriatric unit was successfully associated with both a reduction in antibiotic use and a clear reduction in the incidence of HO-CDI in the geriatric rehabilitation unit.
Bioengineered Nisin Derivative M17Q Has Enhanced Activity against Staphylococcus epidermidis
Authors: Twomey Ellen , Hill Colin , Field Des , Begley Maire
Published: June 2020

Staphylococcus epidermidis is frequently implicated in medical device-related infections. As a result of this, novel approaches for control of this opportunistic pathogen are required. We examined the ability of the natural peptide nisin A, produced by Lactococcus lactis, to inhibit S. epidermidis. In addition, a bank of 29 rationally selected bioengineered L. lactis strains were examined with the aim of identifying a nisin derivative with enhanced antimicrobial activity. Agar-based deferred antagonism assays revealed that wild type nisin A inhibited all 18 S. epidermidis strains tested. Larger zones of inhibition than those obtained from the nisin A producing L. lactis strain were observed for each derivative producer against at least one S. epidermidis strain tested. Six derivative producing strains, (VGA, VGT, SGK, M21A, M17Q, AAA), gave larger zones against all 18 strains compared to the wildtype producing strain. The enhanced bioactivity of M17Q was confirmed using well diffusion, minimum inhibitory concentration (MIC) and a broth-based survival assays. Biofilm assays were performed with plastic microtiter plates and medical device substrates (stainless-steel coupons and three catheter materials). The presence of nisin A significantly reduce the amount of biofilm formed on all surfaces. M17Q was significantly better at reducing biofilm production than nisin A on plastic and stainless-steel. Finally, M17Q was significantly better than nisin A at reducing bacterial numbers in a simulated wound fluid. The findings of this study suggest that nisin and bioengineered derivatives warrant further investigation as potential strategies for the control of S. epidermidis.
Paclitaxel, Imatinib and 5-Fluorouracil Increase the Unbound Fraction of Flucloxacillin In Vitro
Authors: Stolte Maximilian , Ali Weaam , Jänis Janne , Gessner Andre’ , El-Najjar Nahed
Published: June 2020

Flucloxacillin (FLU), an isoxazolyl penicillin, is widely used for the treatment of different bacterial infections in intensive care units (ICU). Being highly bound to plasma proteins, FLU is prone to drug-drug interactions (DDI) when administered concurrently with other drugs. As FLU is binding to both Sudlow’s site I and site II of human serum albumin (HSA), competitive and allosteric interactions with other drugs, highly bound to the same sites, seem conceivable. Knowledge about interaction(s) of FLU with the widely used anticancer agents paclitaxel (PAC), imatinib (IMA), and 5-fluorouracil (5-FU is scarce. The effects of the selected anticancer agents on the unbound fraction of FLU were evaluated in pooled plasma as well as in HSA and α-1-acid glycoprotein (AGP) samples, the second major drug carrier in plasma. FLU levels in spiked samples were analyzed by LC-MS/MS after ultrafiltration. Significant increase in FLU unbound fraction was observed when in combination with PAC and IMA and to a lesser extent with 5-FU. Furthermore, significant binding of FLU to AGP was observed. Collectively, this is the first study showing the binding of FLU to AGP as well as demonstrating a significant DDI between PAC/IMA/5-FU and FLU.
Public Hospital Pharmacists’ Perceptions and Knowledge of Antibiotic Use and Resistance: A Multicenter Survey
Authors: Tang Kai Lun , Teoh Tsyr Fen , Ooi Theng Theng , Khor Wei Ping , Ong Sook Yee , Lim Phin Phin , Abdul Karim Sarah , Tan Sherene Su Ann , Ch’ng Pao Pao , Choong Yen Ching , Foong Weng Siang , Ganesan Sunitha , Khan Amer Hayat , Ming Long Chiau
Published: June 2020

Antimicrobial Stewardship Program (ASP) has been implemented in major public hospitals in Malaysia, with pharmacists playing a key role in ensuring the appropriate use of antibiotics. This survey aimed to assess the practices, perceptions, and knowledge of public hospital pharmacists on antibiotic use and resistance. A cross-sectional survey involving pharmacists from six public hospitals in Penang was conducted using a self-administered validated questionnaire. The majority of pharmacists perceived that polypharmacy (92%, n = 270) and overuse of broad-spectrum antibiotics (85%, n = 252) can potentially induce resistance of microorganisms and that ensuring the rational use of antibiotics is a shared responsibility between clinicians and pharmacists (94%, n = 278). A large majority of the pharmacists think that formal training in infectious disease should be a pre-requisite for pharmacists in ASP (93%, n = 273). In terms of antibiotic selection, the availability of antibiotics in hospital (81%, n = 234) and patient’s clinical condition (68%, n = 196) are more of a concern to the pharmacists. A total of 65% of the respondents (n = 192) demonstrated good levels of knowledge with a mean knowledge score of 10.1 out of 13 (95% CI: 9.95; 10.31). Pharmacists from the managerial level, ward pharmacy, in-patient, and medication therapy adherence clinic (MTAC) unit had better knowledge of antibiotics compared to pharmacists from other units (p < 0.001). Antibiotic knowledge gap had been identified among pharmacists in different work settings, and longer years of service does not warrant good antibiotic knowledge.
Candidiasis and Mechanisms of Antifungal Resistance
Authors: Bhattacharya Somanon , Sae-Tia Sutthichai , Fries Bettina C.
Published: June 2020

Candidiasis can be present as a cutaneous, mucosal or deep-seated organ infection, which is caused by more than 20 types of Candida sp., with C. albicans being the most common. These are pathogenic yeast and are usually present in the normal microbiome. High-risk individuals are patients of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS), organ transplant, and diabetes. During infection, pathogens can adhere to complement receptors and various extracellular matrix proteins in the oral and vaginal cavity. Oral and vaginal Candidiasis results from the overgrowth of Candida sp. in the hosts, causing penetration of the oral and vaginal tissues. Symptoms include white patches in the mouth, tongue, throat, and itchiness or burning of genitalia. Diagnosis involves visual examination, microscopic analysis, or culturing. These infections are treated with a variety of antifungals that target different biosynthetic pathways of the pathogen. For example, echinochandins target cell wall biosynthesis, while allylamines, azoles, and morpholines target ergosterol biosynthesis, and 5-Flucytosine (5FC) targets nucleic acid biosynthesis. Azoles are commonly used in therapeutics, however, because of its fungistatic nature, Candida sp. evolve azole resistance. Besides azoles, Candida sp. also acquire resistance to polyenes, echinochandins, and 5FC. This review discusses, in detail, the drug resistance mechanisms adapted by Candida sp.
Opportunities and Challenges for Improving Anti-Microbial Stewardship in Low- and Middle-Income Countries; Lessons Learnt from the Maternal Sepsis Intervention in Western Uganda
Authors: Ackers Louise , Ackers-Johnson Gavin , Seekles Maaike , Odur Joe , Opio Samuel
Published: June 2020

This paper presents findings from an action-research intervention designed to identify ways of improving antimicrobial stewardship in a Ugandan Regional Referral Hospital. Building on an existing health partnership and extensive action-research on maternal health, it focused on maternal sepsis. Sepsis is one of the main causes of maternal mortality in Uganda and surgical site infection, a major contributing factor. Post-natal wards also consume the largest volume of antibiotics. The findings from the Maternal Sepsis Intervention demonstrate the potential for remarkable changes in health worker behaviour through multi-disciplinary engagement. Nurses and midwives create the connective tissue linking pharmacy, laboratory scientists and junior doctors to support an evidence-based response to prescribing. These multi-disciplinary ‘huddles’ form a necessary, but insufficient, grounding for active clinical pharmacy. The impact on antimicrobial stewardship and maternal mortality and morbidity is ultimately limited by very poor and inconsistent access to antibiotics and supplies. Insufficient and predictable stock-outs undermine behaviour change frustrating health workers’ ability to exercise their knowledge and skill for the benefit of their patients. This escalates healthcare costs and contributes to anti-microbial resistance.
Cadmium-Induced Oxidative Stress: Focus on the Central Nervous System
Authors: Branca Jacopo J. V. , Fiorillo Claudia , Carrino Donatello , Paternostro Ferdinando , Taddei Niccolò , Gulisano Massimo , Pacini Alessandra , Becatti Matteo
Published: June 2020

Cadmium (Cd), a category I human carcinogen, is a well-known widespread environmental pollutant. Chronic Cd exposure affects different organs and tissues, such as the central nervous system (CNS), and its deleterious effects can be linked to indirect reactive oxygen species (ROS) generation. Since Cd is predominantly present in +2 oxidation state, it can interplay with a plethora of channels and transporters in the cell membrane surface in order to enter the cells. Mitochondrial dysfunction, ROS production, glutathione depletion and lipid peroxidation are reviewed in order to better characterize the Cd-elicited molecular pathways. Furthermore, Cd effects on different CNS cell types have been highlighted to better elucidate its role in neurodegenerative disorders. Indeed, Cd can increase blood–brain barrier (BBB) permeability and promotes Cd entry that, in turn, stimulates pericytes in maintaining the BBB open. Once inside the CNS, Cd acts on glial cells (astrocytes, microglia, oligodendrocytes) triggering a pro-inflammatory cascade that accounts for the Cd deleterious effects and neurons inducing the destruction of synaptic branches.
Detection of Antioxidant Phytochemicals Isolated from Camellia japonica Seeds Using HPLC and EPR Imaging
Authors: Saenjum Chalermpong , Pattananandecha Thanawat , Nakagawa Kouichi
Published: June 2020

In this study, we investigated the formation of stable radicals and compounds related to antioxidants in Camellia japonica seeds using high performance liquid chromatography (HPLC) and X-band electron paramagnetic resonance imaging (EPRI). The C. japonica seed coat extracts exhibited antioxidant activity in both in vitro and cell-based studies. The extracts inhibited reactive oxygen and reactive nitrogen species production in cell-based studies. HPLC chromatograms indicated that hydrophilic antioxidant compounds—namely, gallic acid, gallocatechin, epigallocatechin, caffeine, catechin, epicatechin, and epicatechin gallate—were found in the methanolic extract. Lipophilic antioxidant compounds—including α-tocopherol, γ-tocopherol, β-tocopherol, δ-tocopherol, α-tocotrienol, γ-tocotrienol, and δ-tocotrienol—were found in the hexane extract. EPRI primarily detected paramagnetic species in seed coats. These radical species were stable organic radicals based on the peak-to-peak line width and g-values. The signals from these stable radicals were strong and stable with a g-value of 2.002. Noninvasive EPRI of the radicals present in C. japonica seeds indicated that the stable radicals were essentially located in the seed coats. The EPRI of the cotyledon demonstrated that additional radicals were localized at an apex of the cotyledon. The results indicated that the stable radicals detected by EPRI and the hydrophilic and lipophilic antioxidant compounds analyzed by HPLC were related to antioxidant reactants and products.
Retinal Nerve Fiber Layer Thickness and Oxidative Stress Parameters in Migraine Patients without Aura: A Pilot Study
Authors: Bulboacă Adriana Elena , Stănescu Ioana C. , Bolboacă Sorana D. , Bulboacă Angelo C. , Bodizs Gyorgy I. , Nicula Cristina A.
Published: June 2020

Background: Migraine is one of the most common disorders and its pathophysiological mechanisms are still under research, oxidative stress being emphasized as an important contributor. This study aimed to analyze the retinal nerve fiber layer (RNFL) thickness and oxidative/anti-oxidant balance in migraine patients. Methods: Two groups of subjects were evaluated: a group of patients with migraine and a control group of healthy volunteers. RNFL thickness was assessed for all subjects by the ocular coherence tomography spectral domain (OCT-SD). The oxidative stress parameter, namely nitric oxide (NOx), malondialdehyde (MDA), and total oxidative stress (TOS) were assessed. The antioxidant capacity of plasma was evaluated by assessing the level of catalase, and total anti-oxidative (TOS) capacity. Migraine severity was graded using the Migraine Disability Assessment Score (MIDAS) questionnaire. Results: All the oxidative stress parameters (NOx, MDA, and TOS) were significantly increased, and both parameters for anti-oxidative status were significantly decreased in the migraine group compared with the control group (p < 0.0001). Significant correlations with all the quadrants and different oxidative stress parameters were found, most involved being temporal quadrant. A significant positive correlation between catalase and macular RNFL thickness (inner ring, temporal quadrant) in migraine patients, for both eyes, was observed (p = 0.014 for the right eye and p = 0.12 for the left eye). Conclusion: The assessment of the oxidative stress/anti-oxidative balance together with RFLN thickness can constitute a promising method to evaluate the progression of the diseases. It can also contribute to the estimation of the efficiency of various therapies targeting oxidative stress and associated inflammation.
Chemical Composition and Biological Activities of the Nord-West Romanian Wild Bilberry (Vaccinium myrtillus L.) and Lingonberry (Vaccinium vitis-idaea L.) Leaves
Authors: Ștefănescu Bianca-Eugenia , Călinoiu Lavinia Florina , Ranga Floricuța , Fetea Florinela , Mocan Andrei , Vodnar Dan Cristian , Crișan Gianina
Published: June 2020

This study was performed to evaluate and compare the in vitro antioxidant, antimicrobial, and antimutagenic activities, and the polyphenolic content of the Nord-West Romanian wild bilberry (Vaccinium myrtillus L.) and lingonberry (Vaccinium vitis-idaea L.) leaves from three different natural habitats (Smida, Turda, Borsa). In the case of both species, the flavanols level was higher in Smida habitat (altitude 1100 m), whereas quercetin derivates were more abundant in Borsa habitat (altitude 850 m). The bilberry leaf extracts contained in the highest amounts the feruloylquinic acid (59.65 ± 0.44 mg/g for Borsa habitat) and rutin (49.83 ± 0.63 mg/g for Borsa habitat), and showed relevant 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity (271.65 mM Trolox/100 g plant material for Borsa habitat, 262.77 mM Trolox/100 g plant material for Smida habitat, and 320.83 mM Trolox/100 g plant material for Turda habitat), for all the three extracts. Gallocatechin was the dominant flavanol in lingonberry species, with the highest amount being registered for Smida habitat (46.81 ± 0.3 mg/g), revealing a DPPH antioxidant activity of 251.49 mM Trolox/100 g plant material. The results obtained in the antimicrobial tests showed that the best inhibitory effect among bilberry species was attributed to the Turda (altitude 436 m) and Smida locations, against both Gram-positive and Gram-negative bacterial strains. For lingonberry, the differences in habitat did not influence the antibacterial effect, but the antifungal effect, only in the case of Candida zeylanoides. A strong antimutagenic effect was registered by the bilberry leaves toward Salmonella typhimurium TA100. Our study may be able to provide a better understanding of the correlation between natural habitat conditions and the accumulation of secondary metabolites and their related bioactivities in studied leaves.
Influence of Long-Term Fasting on Blood Redox Status in Humans
Authors: Wilhelmi de Toledo Françoise , Grundler Franziska , Goutzourelas Nikolaos , Tekos Fotios , Vassi Eleni , Mesnage Robin , Kouretas Demetrios
Published: June 2020

Fasting is increasingly practiced to improve health and general well-being, as well as for its cytoprotective effects. Changes in blood redox status, linked to the development of a variety of metabolic diseases, have been recently documented during calorie restriction and intermittent fasting, but not with long-term fasting (LF). We investigated some parameters of the blood redox profile in 109 subjects before and after a 10-day fasting period. Fasting resulted in a significant reduction in body weight, improved well-being and had a beneficial modulating effect on blood lipids and glucose regulation. We observed that fasting decreased lipid peroxidation (TBARS) and increased total antioxidant capacity (TAC) in plasma, concomitant with a uric acid elevation, known to be associated with fasting and did not cause gout attacks. Reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx) and catalase in erythrocytes did not show significant changes. In addition, reduction in body weight, waist circumference, and glucose levels were associated to a reduced lipid peroxidation. Similar results were obtained by grouping subjects on the basis of the changes in their GSH levels, showing that a period of 10 days fasting improves blood redox status regardless of GSH status in the blood.
The Role of Nutri(epi)genomics in Achieving the Body’s Full Potential in Physical Activity
Authors: Petracci Irene , Gabbianelli Rosita , Bordoni Laura
Published: June 2020

Physical activity represents a powerful tool to achieve optimal health. The overall activation of several molecular pathways is associated with many beneficial effects, mainly converging towards a reduced systemic inflammation. Not surprisingly, regular activity can contribute to lowering the “epigenetic age”, acting as a modulator of risk toward several diseases and enhancing longevity. Behind this, there are complex molecular mechanisms induced by exercise, which modulate gene expression, also through epigenetic modifications. The exercise-induced epigenetic imprint can be transient or permanent and contributes to the muscle memory, which allows the skeletal muscle adaptation to environmental stimuli previously encountered. Nutrition, through key macro- and micronutrients with antioxidant properties, can play an important role in supporting skeletal muscle trophism and those molecular pathways triggering the beneficial effects of physical activity. Nutrients and antioxidant food components, reversibly altering the epigenetic imprint, have a big impact on the phenotype. This assigns a role of primary importance to nutri(epi)genomics, not only in optimizing physical performance, but also in promoting long term health. The crosstalk between physical activity and nutrition represents a major environmental pressure able to shape human genotypes and phenotypes, thus, choosing the right combination of lifestyle factors ensures health and longevity.
Effect of Grafting on the Production, Physico-Chemical Characteristics and Nutritional Quality of Fruit from Pepper Landraces
Authors: Gisbert-Mullor Ramón , Ceccanti Costanza , Gara Padilla Yaiza , López-Galarza Salvador , Calatayud Ángeles , Conte Giuseppe , Guidi Lucia
Published: June 2020

Grafting is a widely utilized agronomical technique to improve yield, disease resistance, and quality of fruit and vegetables. This work aims to assess the effect of grafting and fruit ripening on the production, physico-chemical characteristics, and nutritional quality of fruit from Spanish local pepper landraces. Landraces “Cuerno,” “Sueca,” and “Valencia” were used as scions, and “NIBER®” as the rootstock. Two ripening stages of the fruits were sampled: green and red. Grafting improved the yield and marketable quality and did not negatively influence the physico-chemical and nutritional characteristics of the fruit. It was noteworthy that the bioactive compound contents and antioxidant capacity were more related to maturity stage and genotype, and red fruit had a higher antioxidant capacity than green fruit. However, in all the scions, grafting significantly enhanced lycopene content in both red and green fruit. Another important effect of grafting was the volatile compound composition evidenced by discriminant analyses, which was characterized for the first time in the fruit of these landraces. The rootstock and scion combination could be a way to improve not only the production, but also the fruit quality of peppers.
Research Advances in the Use of Bioactive Compounds from Vitis vinifera By-Products in Oral Care
Authors: Bogdan Cătălina , Pop Anca , Iurian Sonia M. , Benedec Daniela , Moldovan Mirela L.
Published: June 2020

Oral health is considered an important factor of general health and it contributes to the quality of life. Despite the raising awareness of preventive measures, the prevalence of oral health conditions continues to increase. In this context, a growing interest in investigating natural resources like Vitis vinifera (V. vinifera) phenolic compounds (PhCs) as oral health promoters has emerged. This paper aims to review the evidence about the bioactivities of V. vinifera by-products in oral health. Up to date, a high number of studies have thoroughly reported the antimicrobial and antiplaque activity of V. vinifera extracts against S. mutans or in multi-species biofilms. Moreover, the bioactive compounds from V. vinifera by-products have been shown to modulate the periodontal inflammatory response and the underlying oxidative stress imbalance induced by the pathogenic bacteria. Considering these beneficial effects, the utility of V. vinifera by-products in the maintaining of oral health and the necessary steps towards the development of oral care products were emphasized. In conclusion, the high potential of V. vinifera by-products could be valorized in the development of oral hygiene products with multi-target actions in the prevention and progression of several oral conditions.
Polymyxins–Curcumin Combination Antimicrobial Therapy: Safety Implications and Efficacy for Infection Treatment
Authors: Dai Chongshan , Wang Yang , Sharma Gaurav , Shen Jianzhong , Velkov Tony , Xiao Xilong
Published: June 2020

The emergence of antimicrobial resistance in Gram-negative bacteria poses a huge health challenge. The therapeutic use of polymyxins (i.e., colistin and polymyxin B) is commonplace due to high efficacy and limiting treatment options for multidrug-resistant Gram-negative bacterial infections. Nephrotoxicity and neurotoxicity are the major dose-limiting factors that limit the therapeutic window of polymyxins; nephrotoxicity is a complication in up to ~60% of patients. The emergence of polymyxin-resistant strains or polymyxin heteroresistance is also a limiting factor. These caveats have catalyzed the search for polymyxin combinations that synergistically kill polymyxin-susceptible and resistant organisms and/or minimize the unwanted side effects. Curcumin—an FDA-approved natural product—exerts many pharmacological activities. Recent studies showed that polymyxins–curcumin combinations showed a synergistically inhibitory effect on the growth of bacteria (e.g., Gram-positive and Gram-negative bacteria) in vitro. Moreover, curcumin co-administration ameliorated colistin-induced nephrotoxicity and neurotoxicity by inhibiting oxidative stress, mitochondrial dysfunction, inflammation and apoptosis. In this review, we summarize the current knowledge-base of polymyxins–curcumin combination therapy and discuss the underlying mechanisms. For the clinical translation of this combination to become a reality, further research is required to develop novel polymyxins–curcumin formulations with optimized pharmacokinetics and dosage regimens.