bluuebunny/crossref_metadata_2025 huggingface dataset:
Synergistic effects of anlotinib and DDP on breast cancer: targeting the VEGF/JAK2/STAT3 axis
Authors: Zhang Hongmei , Liu Chunling , Jin Ye , Wang Zheng , Guan Yi , Jia Zhenxian , Cui Tong , Zhang Zhi , Zhang Xuemei
Published: October 2024

Background

Anlotinib, a highly selective inhibitor of VEGFR2, has demonstrated significant anti-tumor effects in various cancers. However, its potential synergistic effects with DDP (cisplatin) in breast cancer (BRCA) remain to be fully elucidated. This study aims to discover the therapeutic efficacy of anlotinib on BRCA, specifically the synergistic effects with DDP, and to elucidate the underlying molecular mechanisms.

Methods

BRCA cells were treated with anlotinib and/or DDP. The proliferation, migration and invasion capabilities of BRCA cells were evaluated using CCK-8 assays, cell cycle distribution, clone formation assays, wound healing assays and transwell assays. Cell apoptosis was detected by flow cytometry technique and Hoechst33342 fluorescence staining. The potential mechanism of anlotinib in the development of BRCA was predicted through bioinformatics analysis, and the mRNA or protein levels were subsequently quantified using qPCR, immunofuorescence and western blot. The anti-breast cancer efficacy of anlotinib was evaluated in vivo using a xenograft tumor model.

Results

Our findings reveal that increased VEGFA expression in BRCA patients is associated with poorer prognosis, underscoring the need for targeted therapeutic strategies. We also demonstrate that both anlotinib and DDP independently inhibit BRCA cell growth, migration, and invasion, while their combination exhibits a synergistic effect, significantly enhancing the inhibition of these oncogenic processes. This synergy is further evident through the induction of apoptosis and autophagy in BRCA cells. Mechanistically, anlotinib’s effectiveness is linked to its inhibition of the JAK2/STAT3 pathway, a critical axis in BRCA progression. In vivo study further support these results, showing that anlotinib markedly inhibits tumor growth in xenografted mice.

Conclusion

This study confirms the efficacy of anlotinib or in combination with DDP and elucidates the mechanism behind anlotinib’s effectiveness, highlighting its role in inhibiting the JAK2/STAT3 pathway.
Research on the characteristics of the Helium plasma beam in HIT-PSI
Authors: Huang Tao , Nie Qiuyue , Jiang Tao , Chen Cheng , Liu Yang , Gao Jinming , Cai Laizhong , Zhao Xu , Wang Xiaogang
Published: October 2024

As a high heat flux linear plasma device designed for studying divertor materials in future fusion reactors, HIT-PSI(Plasma Surface Interaction device at Harbin Institute of Technology) has been successfully constructed and has maintained stable operation since its completion. The characteristics of He plasma beams in HIT-PSI are investigated by emission spectroscopy and an infrared camera, with preliminary irradiation experiments conducted by bombarding tungsten with the beam. For relatively conservative discharge parameters, HIT-PSI achieved a steady-state heat flux capacity of ∼40 MW/m2 using infrared measurements, with the full width at half maximum (FWHM) of the heat flux beam reaching 4 mm. These characteristics make HIT-PSI an advanced platform for testing divertor materials and plasma-facing components, providing essential experimental supports for research and development of high-performance divertor materials.
Effects of UV-A/B/C on flavonoids and related synthetic enzymes in Tetrastigma hemsleyanum
Authors: Li Shan , Xia Jingqing , Liu Shouzan , Li Zhe , Shen Qiong , Yang Feng , Liu Xinhong , Bai Yan
Published: October 2024

Introduction

Tetrastigma hemsleyanum is a folk and rare medicinal plant, and specifically, it is distributed in the south, China. To investigate the cumulative properties of its medicinal components, we examined the effect of UV light on flavonoid content and related enzyme activity changes in T. hemsleyanum.

Methods

The leaves and tubers were treated with UV-A, UV-B and UV-C for 1 h, 1L/23D h, 3 h and 3L/21D h (D represents darkness treatment). High-performance liquid chromatography (HPLC) analysis showed that the content of many flavonoids decreased significantly during UV-A treatment, increased after UV-B and UV-C irradiation and accumulated again after darkness treatment.

Results

In the root tubers of the UV-A group, naringin content in the 3L/21D h group (0.069 μg/g) was 16.30 times higher than that of 3 h group (0.0042 μg/g). The rutin content was elevated after UV irradiation but was not detected in the CK group. The test results of the enzyme-linked kit indicated that the activities of many enzymes were higher in the UV-A and UV-B irradiation groups than those in the CK group, but the results were reversed in the UV-C treatment. After darkness treatment, the activities of most enzymes were higher than those with UV irradiation alone; F3’5’H activity in the 3L/21D h group (97.25 U/L) was 1.24 times higher than that in the 3 h group (78.12 U/L) in the UV-A-treated group.

Discussion

The study results suggest that appropriate UV-B and UV-C irradiation, as well as darkness supplementation, had a promotive effect on flavonoids in the leaves and root tubers of T. hemsleyanum. Additionally, UV irradiation and darkness treatment enhanced the activity of most enzymes.
Alignment of color discrimination in humans and image segmentation networks
Authors: Hernández-Cámara Pablo , Daudén-Oliver Paula , Laparra Valero , Malo Jesús
Published: October 2024

The experiments allowed by current machine learning models imply a revival of the debate on the causes of specific trends of human visual psychophysics. Machine learning facilitates the exploration of the effect of specific visual goals (such as image segmentation) by different neural architectures in different statistical environments in an unprecedented manner. In this way, (1) the principles behind psychophysical facts such as the non-Euclidean nature of human color discrimination and (2) the emergence of human-like behaviour in artificial systems can be explored under a new light. In this work, we show for the first time that the tolerance or invariance of image segmentation networks for natural images under changes of illuminant in the color space (a sort of insensitivity region around the white) is an ellipsoid oriented similarly to a (human) MacAdam ellipse. This striking similarity between an artificial system and human vision motivates a set of experiments checking the relevance of the statistical environment on the emergence of such insensitivity regions. Results suggest, that in this case, the statistics of the environment may be more relevant than the architecture selected to perform the image segmentation.
Mindfulness and mental health: the importance of a clinical intervention to prevent the effects of a traumatic event. A pilot study
Authors: Caldiroli Cristina Liviana , Procaccia Rossella , Negri Attà , Mangiatordi Andrea , Sarandacchi Silvia , Antonietti Alessandro , Castiglioni Marco
Published: October 2024

Numerous research studies show that mindfulness can mitigate the negative impact of trauma on mental health by reducing symptoms of anxiety, mediating the relationship between trauma exposure and mental health, and treating symptoms resulting from traumatic events. During the COVID-19 pandemic, which was considered a traumatic event, the wellbeing of adults and children was severely compromised. Although children seem less vulnerable to the physical effects of the virus, this does not seem to be true for the psychological effects. Indeed, a prolonged period of loss of family activities and routines can have a negative impact on the mental health of children and adolescents. To investigate how mindfulness can help preschool children cope with the effects of COVID-19, a study was conducted on 46 children aged 4–5 years. The programme, based on the work of Jon Kabat-Zinn and adapted to the age of the participants, consisted of eight weekly 45-min sessions. Qualitative and quantitative results showed positive feedback, indicating that mindfulness helps children make sense of their experiences and achieve functional post-traumatic growth. This approach is seen as a challenge to guide children toward the restoration of psychological wellbeing, which is essential for good psychological balance.
Only consciousness truly exists? Two problems for IIT 4.0’s ontology
Authors: Cea Ignacio , Negro Niccolo , Signorelli Camilo Miguel
Published: October 2024

In this article we present two ontological problems for the Integrated Information Theory of Consciousness 4.0: what we call the (i) the intrinsicality 2.0 problem, and (ii) the engineering problem. These problems entail that truly existing, conscious entities can depend on, and be engineered from, entities that do not objectively exist, which is problematic: if something does not exist in objective reality (i.e., in itself, independently of another entity’s consciousness), then it seems that it cannot be part of the material basis and determinants of other entities that do exist on their own. We argue that the core origin of these problems lies in IIT’s equation between true existence and phenomenal existence (consciousness), and the corresponding ontological exclusion of non-conscious physical entities (i.e., extrinsic entities) from objective reality. In short, these two problems seem to show that IIT should reconsider the ontological status of these extrinsic entities, because they need to exist objectively to account for the ontological implications of the scenarios we present here, which are permitted by the operational framework of the theory.
Influence of personality disorders on sexual behaviours and response to treatment of psychogenic erectile dysfunction in phosphodiesterase 5 inhibitor non-responders
Authors: Cabello-García Marina , Sánchez-Sandoval Yolanda , García-Rojas Antonio Daniel
Published: October 2024

Background

Personality disorders may influence sexual behaviours and sexual dysfunction.

Aim

Our main objective was to analyse the influence of personality disorders (PDs) in patients with erectile dysfunction (ED) of psychological origin that fail to respond to andrological treatment with Phosphodiesterase-5 inhibitors (IPDE5), assessing whether there are differences in sexual behaviours and response to psychosexual treatment.

Methods

The research is designed as an ex post facto retrospective study with two groups. A control group of 23 men with ED without personality disorders and a group of 51 men with both ED and PDs.

Results

In the case sample, 34.30% of the participants presented more than one personality disorder. No significant differences were found in sexual behaviours except for heteromasturbation (men without PDs masturbated their partners more to satisfy them than men with PDs), and men with PDs considered themselves less premature ejaculators than the control group. Finally, 82.14% of the control group did well with psychosexual therapy compared to 53.85% of the PDs group.

Conclusion

Psychosexual treatment of ED has a worse outcome if the men also have PDs. Strengths and Limitations: from a clinical standpoint, it is important to assess the presence of personality disorders in men with ED and to implement psychosexual strategies to improve the response to treatment in these cases. Confirmation of the results with a much larger sample becomes necessary.
The evolution of psychotherapy: from Freud to prescription digital therapeutics
Authors: Docherty John P. , Colbert Brett M.
Published: October 2024

The evolution of psychotherapeutic treatments from Freud to digitally administered evidence-based treatments reflects a history of progressive advance. This history is characterized by identification of problems with the current state of the art, followed by solutions inspired and supported by advances in basic science and technology leading to subsequent recognition of other limitations revealed by the new advance. The common thread running through this process is (a) increasing specificity of the psychotherapeutic interventions, (b) increasing evidence of efficacy and safety, (c) increasing integrity and reliability in the delivery of the intervention, (d) increased equality of access, and (e) recognition of the need for regulation to provide protection for the public from unsafe or ineffective products. This evolution of psychotherapeutic treatments, not surprisingly, has been foreshadowed by the precursor history of the evolution of pharmacologic treatment. Although intellectual history is lumpy and does not sort itself into discrete and coherent epochs, such sorting is a useful heuristic for describing the advance of medicine and the therapeutic enterprise. This paper will discuss six successive epochs of psychotherapy. For each it will discuss the problem of the preceding era it sought to solve, the advance it brought to the field, the emerging science and technology that supported that advance, and the precursor development in pharmacological treatments that foreshadowed that epoch of psychotherapy. Finally, it will conclude with some observations about the proximate future.
Wireless sensor network-based health monitoring and smart home assistance for the older adults in sports and wellness towns
Authors: Yang Dapeng , Wang Junqi , Huang Li , Wang Tongling
Published: October 2024

Background

Integrating technology with rural development is essential for addressing the unique challenges faced by aging populations in rural areas. China’s national rural revitalization strategy emphasizes the importance of developing characteristic towns that focus on health and wellness demands, particularly for the older adults. However, there is a gap in the literature regarding the systematic use of technology to support the health and daily living needs of this demographic.

Objective

This study aims to bridge this gap by proposing a wireless sensor network (WSN)-based system that integrates health monitoring and smart home assistance, specifically tailored for older adults residents in sports and wellness towns.

Methods

The system’s design involves collecting and analyzing historical activity data and basic physiological parameters from older adults residents’ homes. The data are cleaned, combined and stored to prepare it for behavior analysis, which is vital for controlling smart home equipment. Preliminary health assessments are conducted using the collected physiological data. A hybrid network leveraging Sub-G and Wi-Fi technologies is optimized for the purposes of collecting and uploading data, based on a comparative analysis of wireless communication options.

Significance

This study significantly contributes to the advancement of sports and wellness towns by promoting healthy aging through cutting-edge technological solutions.
Educational attainment, brain cortical structure, and sarcopenia: a Mendelian randomization study
Authors: Zhang Yunqing , Wang Ruideng , Chen Zhengyang , Zhou Fang , Su Shilong
Published: October 2024

Background

Previous observational studies have suggested associations between high-level educational attainment (EA) and a lower risk of sarcopenia. However, the causality inferred from those studies was subjected to residual confounding and reverse causation. The protective effect of EA on sarcopenia may be mediated via changes in brain cortical structure. The aim of this study was to use a two-step Mendelian randomization (MR) analysis to illustrate the causal relationship between EA, brain cortical structure, and sarcopenia.

Methods

Instrumental variables at the genome-wide significance level were obtained from publicly available datasets, and inverse variance weighted as the primary method was used for MR analysis. We perform several sensitivity analyses, including Cochran Q test, MR-Egger intercept test, leave-one-out analyses, and MR Pleiotropy Residual Sum and Outlier to evaluate the reliability of the results.

Results

EA was causally associated with increased appendicular lean mass (β = 0.25, 95% confidence interval (CI): 0.19 to 0.31, p = 2.25 × 10−15), hand grip strength (left: β = 0.042, 95% CI: 0.013 to 0.071, p = 4.77 × 10−3 and right: β = 0.050, 95% CI: 0.022 to 0.079, p = 5.17 × 10−4), and usual walking pace (β = 0.20, 95% CI: 0.18 to 0.22, p = 6.16 × 10−83). In addition, EA was associated with increased brain cortical surface area (β = 4082.36, 95% CI: 2513.35 to 5681.38, p = 3.40 × 10−7) and cortical thickness (TH) (β = 0.014, 95% CI: 0.0045 to 0.023, p = 3.45 × 10−3). Regarding the causal effect of EA on usual walking pace, the mediatory effect of TH was 0.0069 and the proportion of mediation by TH was 3.43%.

Conclusion

The study will have revealed the protective causal effect of EA on sarcopenia, which provides a reference for the prevention of sarcopenia at the public health level. We also will have found EA could affect the brain cortical structure, and the brain cortical structure could mediate the protective effect of EA against sarcopenia risk.
Professional development: a mixed methods study of Masters of Public Health alumni
Authors: Baron-Epel Orna , Douvdevany Yana , Ivancovsky-Wajcman Dana , Barach Paul , Bashkin Osnat , Czabanowska Katarzyna , Dopelt Keren , Davidovitch Nadav , Jakubowski Szczepan , MacLeod Fiona , Malowany Maureen , Okenwa-Emegwa Leah , Peled-Raz Maya , Zelber-Sagi Shira
Published: October 2024

Introduction

We examined the perceptions of the Master of Public Health (MPH) degree graduates regarding their personal competencies, job performance and professional development using a mixed method, explanatory sequential design.

Methods

A cross-sectional, self-administered questionnaire of the Haifa School of Public Health alumni who graduated between 2005 and 2022 was disseminated to 849 graduates between March and June 2022, from which 127 responded (response rate: 14.90%). This was followed by 24 in-depth interviews with alumni from the same sample (conducted between November 2022 and March 2023).

Results

The sample included 74.8% of females with a mean age of 40.7 years, 35% of alumni agreed that the MPH degree helped them attain a promotion in their present position (in rank or salary), and 63.8% felt that the degree helped them improve their job performance and contribute to their current workplace. Most (80.3%) alumni reported not changing jobs after graduation. The interview themes revealed that the MPH contributed to their personal and professional lives, provided them with a holistic view of public health and health systems, and improved their in-depth scientific skills. The main reported barriers to professional development included missing core competencies, low salaries, and a lack of information regarding suitable jobs. Surprisingly, an MPH was not a requirement for some public health sector jobs. Alumni reported that the MPH degree contributed to improving many graduates’ careers and satisfaction levels and to build their leadership competencies in public health.

Discussion

There seems to be a lack of coordination between the academic curriculum and the jobs available for alumni, hindering better alumni professional development. Regular discussions, information sharing, and curriculum refinements between MPH program leaders and health sector leaders might help address many of the concerns of MPH degree graduates.
Community case study for surveillance and early case-detection of SARS-CoV-2 infections across high-risk key populations: the Sentinella programme
Authors: Tebon Maela , Davis Ruth Joanna , Savoldi Alessia , Soriolo Nicola , Walters Sarah Elizabeth Jane , Nosè Michela , Barbui Corrado , Turrini Giulia , Danese Elisa , Lippi Giuseppe , Cecchetto Riccardo , Mazzariol Annarita , Gibellini Davide , Caliskan Gulser , Marchetti Pierpaolo , Verlato Giuseppe , Princivalle Andrea , Porru Stefano , Tacconelli Evelina , De Nardo Pasquale
Published: October 2024

At the beginning of the COVID-19 pandemic, an ad hoc organisational framework was established between academic, local government and community partners to implement the “Sentinella – Identify, Trace and Prevent” screening programme in Verona, north-east Italy. Between September 2020 and May 2021, key populations not covered by any screening policies at the local and national level were screened for SARS-CoV-2. Target populations were: older adult residents (males >65 years and females >75 years), bus and taxi drivers, social workers, supermarket employees, hospital cleaning and catering staff, researchers working in the local hospitals, students, and people experiencing homelessness (PEH). Five dedicated swab clinics, home testing facilities, and one mobile clinic were activated to collect nasopharyngeal swabs. Molecular analysis was performed for all the subjects; an antigen-rapid diagnostic test (Ag-RDT) was also implemented as a point-of-care test for PEH. Medical follow-up, psychological support, and quarantine facilities were organised for subjects who tested positive for SARS-CoV-2. Overall, 2075 subjects participated in the surveillance programme. Amongst these, 1,572 were residents/workers, whilst 503 were PEH. A total of 127 (6.2%) participants tested positive for SARS-CoV-2. Sixty-nine were residents, 58 PEH. The incidence rate was 4 per 10.000 person/day (95% CI 3.1–5.0). The highest prevalence and incidence rates were found amongst supermarket employees (9.7% and 8.5 per 10.000 person/day, 95% CI 3.81–18.86, respectively), followed by hospital cleaning staff (8.1%, 7.6 per 10.000 person/day, CI 95% 4.9–11.7). Regarding PEH, the prevalence of SARS-CoV-2 was 11.5%. All PEH identified as positive were isolated in dedicated shelter facilities. Amongst the 69 residents/workers who were quarantined, 53 were reached for initial psychological support for assessing the presence of any psychological distress or psychiatric pathology. Amongst the subjects evaluated, 10 (18.9%) presented clinically significant psychological discomfort and accessed the stepped-care psychological intervention. The community partnerships played a pivotal role in optimising early case detection. Promotion of testing helped to prevent and contain more efficiently potential clusters through strategic planning, especially for PEH. Insights from the study highlight the importance of community partnerships in public health emergencies, particularly in the context of highly transmissible diseases pathways.
Case study: lessons learned from a community-based early frailty intervention programme in Singapore
Authors: Jayaprakash Kamala Priya , Ngo Rachel , Lee Elianna , Chng Pey Ling , Lee Hengky , Chua Salan , Lee Deborah , Wong Carina , S Viknessh
Published: October 2024

Frailty is a dynamic and evolving state of health which involves the gradual loss of physiological in-built reserves. In Singapore, there is growing interest in delivering frailty intervention programmes at scale in the community to meet the demands of an ageing population. New methods of programme delivery such as community-led models that do not rely on healthcare professional manpower are critical to address this unmet need. In this paper, we describe our experience and some lessons learned from the implementation of a community-based early frailty intervention programme for older adults, delivered for the first time by trained laypersons. From August to September 2022, “Steady Lah,” a community-based early frailty intervention programme with physical activity and nutrition-based elements, was conducted at an Active Ageing Centre in Singapore. A total of 23 participants with mean age of 73.8 years were enrolled in the 12-session programme comprising of progressive strength and balance-based exercises and workshop-based learning focusing on nutrition. In the implementation of this run of “Steady Lah,” modifications were made from a healthcare professional-led model to a trained layperson-led model with additional steps taken to ensure participant safety and assess overall effectiveness when delivered by trained laypersons. Good collaboration between stakeholders in healthcare institutions and the community is necessary to co-develop a model that prioritises the needs of the frail older adults.
Reduce, reinforce, and replenish: safeguarding the early-life microbiota to reduce intergenerational health disparities
Authors: Dai Darlene L. Y. , Petersen Charisse , Turvey Stuart E.
Published: October 2024

Socioeconomic (SE) disparity and health inequity are closely intertwined and associated with cross-generational increases in the rates of multiple chronic non-communicable diseases (NCDs) in North America and beyond. Coinciding with this social trend is an observed loss of biodiversity within the community of colonizing microbes that live in and on our bodies. Researchers have rightfully pointed to the microbiota as a key modifiable factor with the potential to ease existing health inequities. Although a number of studies have connected the adult microbiome to socioeconomic determinants and health outcomes, few studies have investigated the role of the infant microbiome in perpetuating these outcomes across generations. It is an essential and important question as the infant microbiota is highly sensitive to external forces, and observed shifts during this critical window often portend long-term outcomes of health and disease. While this is often studied in the context of direct modulators, such as delivery mode, family size, antibiotic exposure, and breastfeeding, many of these factors are tied to underlying socioeconomic and/or cross-generational factors. Exploring cross-generational socioeconomic and health inequities through the lens of the infant microbiome may provide valuable avenues to break these intergenerational cycles. In this review, we will focus on the impact of social inequality in infant microbiome development and discuss the benefits of prioritizing and restoring early-life microbiota maturation for reducing intergenerational health disparities.
Caregiving burden, social support, and psychological well-being among family caregivers of older Italians: a cross-sectional study
Authors: Bongelli Ramona , Busilacchi Gianluca , Pacifico Antonio , Fabiani Michele , Guarascio Carmela , Sofritti Federico , Lamura Giovanni , Santini Sara
Published: October 2024

Objectives

This study aimed to identify factors affecting the psychological well-being of family caregivers of dependent older adults in Italy. Understanding these variables is essential for designing interventions to prevent negative outcomes in at-risk caregivers. The research explored how varying levels of caregiving burden and types of perceived social support influence psychological well-being.

Methods

A cross-sectional study was conducted among 387 family caregivers of older adults in the Marche region (Italy) between November 2019 and March 2020. Data were collected via a structured questionnaire assessing psychological well-being (WHO-5 Well-Being Index), caregiving burden, and social support (COPE Index). Statistical analyses were performed using Jamovi software, with a significance threshold set at p < 0.05.

Results

A significant negative correlation was found between caregiving burden and psychological well-being [r (364) = − 0.540, p < 0.001], with caregiving burden being a significant predictor of psychological well-being reduction (R2 = 0.290; F = 150, p < 0.001). A threshold value of 2 (on a 1–4 scale) was identified, where caregiving burden predicted a significant reduction in psychological well-being. Conversely, greater perceived social support was positively correlated with better psychological well-being [r (357) = 0.348, p < 0.001] and was a significant predictor of it [R2 = 0.121; F = 49.2, p < 0.001]. Support from social and health services had the most notable impact on psychological well-being. Moreover, social support mitigated the negative impact of caregiving burden on psychological well-being.

Conclusion

The study confirms that high caregiving burden adversely affects caregivers’ psychological well-being, while social support plays a protective role. These findings highlight the need for interventions focused on reducing caregiving burden and enhancing support systems for family caregivers.
Healthcare Internet of Things system implementations for COVID-19 prevention
Authors: Leng Jichao , Lin Zihuai , Yoo Soojeong , Scandurra Gabriella , Dickinson Michael , Vucetic Branka , Wang Audrey P.
Published: October 2024

Background

In response to the widespread transmission of COVID-19 in Australia, healthcare facilities implemented stringent infection control measures, and mandatory and manual screening procedures were introduced to ensure the safety of patients and healthcare staff. However, these necessary measures resulted in imbalances within the healthcare system, a shortage of front-line workers and impacts on patient experience and wait times. The prioritization of infection control measures shifted resources away from routine care, causing delays in accessing necessary healthcare services.

Methods and Findings

To address these challenges, we developed and implemented an Internet of Things (IoT) Smart Screening eGate solution in partnership with a large metropolitan children’s hospital in Australia. This solution integrated a contactless health self-service web app, thermal camera, and physical barrier to automate the COVID-19 health screening and data recording process. During the 3-month pilot period, we deployed the eGate at multiple entrances to the hospital, and monitored the number of users of the system in different periods. We also used a framework of formative evaluation to classify user design challenges within limited resources and improved the design of the eGate to enhance its effectiveness. Our findings show that the IoT eGate solution improved the efficiency of the screening process and reduced the workload and exposure risks of front-line staff and anyone who required access to the hospital. By automating the screening process, we reduced the need for manual screening and minimized contact between individuals, thus reducing the risk of potentially infected.

Conclusion

In conclusion, our pilot study demonstrated the potential of IoT technologies in improving the efficiency and safety of medical facilities during pandemics and provided a series of recommendations for the translation of IoT technologies for medical facilities, including the importance of co-design and collaboration with stakeholders, user-centered design, and ongoing monitoring and evaluation.
Responses of several measures to different intensity levels of upper limb exergames in children with neurological diagnoses: a pilot study
Authors: Goikoetxea-Sotelo Gaizka , van Hedel Hubertus J. A.
Published: October 2024

Background

Therapy intensity is among the most critical factors influencing neurorehabilitative outcomes. Because of its simplicity, time spent in therapy is the most commonly used measure of therapy intensity. However, time spent in therapy is only a vague estimate of how hard a patient works during therapy. Several measures have been proposed to better capture the amount of work a patient puts forth during therapy. Still, it has never been analyzed how these measures respond to changes in therapist-selected exercise intensity in children with neurological conditions.

Objectives

To investigate the response and the reliability of heart rate variability (HRV), skin conductance (SC), activity counts per minute (AC/min), movement repetitions per minute (MOV/min), and perceived exertion to different therapist-tailored intensity levels of upper limb technology-assisted therapy in children with neurological conditions.

Methods

In this pilot cross-sectional study, participants engaged in three personalized, randomized exergame intensity levels (“very easy”, “challenging”, “very difficult”) for eight minutes each. We assessed all measures at each intensity level. The experiment was conducted twice on two consecutive days. We quantified reliability using intra-class correlation coefficients (ICC).

Results

We included 12 children and adolescents aged 11.92 (±3.03) years. HRV, MOV/min, and perceived exertion could differentiate among the three intensity levels. HRV, MOV/min, perceived exertion, and AC/min showed moderate to excellent (0.62 ≤ ICC ≤ 0.98) test-retest reliability.

Conclusion

HRV, MOV/min, and perceived exertion show potential for becoming valid and reliable intensity measures for an upper limb robotic rehabilitative setting. However, studies with larger sample sizes and more standardized approaches are needed to understand these measures’ responses better.
Achieving HIV epidemic control through accelerating efforts to expand access to pre-exposure prophylaxis for people who inject drugs
Authors: Nishimoto Lirica , Olisa Adaobi Lisa , Imohi Philip , Chang Judy , Obermeyer Chris , Kabwali Dama , Akolo Christopher
Published: October 2024

The world is not on track to reach the majority of the UNAIDS 2025 targets, and people who inject drugs (PWID) continue to be left behind, hindered by counterproductive law enforcement practices, punitive laws, economic distress, and social stigma and discrimination. Poor access to HIV pre-exposure prophylaxis (PrEP) among PWID is nested within the limited access to broader harm reduction services, including needle and syringe programs, opioid overdose management, opioid agonist therapy (also known as medication-assisted treatment), and condoms. Among PWID, women who inject drugs are disproportionately affected and face additional gender-based barriers. Intersections between PWID and other key and priority population groups also exist. Although the prioritization of PWID for new PrEP products like the dapivirine vaginal ring and injectable cabotegravir has lagged in research, studies have shown that PWID find injectable and long-acting options acceptable and preferrable, including among women who inject drugs. While new PrEP products introduce new opportunities, equity in access must be assured for optimized impact toward achieving epidemic control. Programming for services must engage and empower PWID community leadership to address the structural barriers to services, implement community-led, differentiated, and integrated service modalities, and offer the choice of all harm reduction options to close the equity gaps in health outcomes. While waiting for necessary evidence and approvals, programs should work together with the PWID community to prioritize, expand, and facilitate efforts and investments toward increased access to and integration of PrEP and all recommended harm reduction services for PWID.
Maize intercropped with Panicum maximum cultivars and Pigeon pea improves silage yield and quality
Authors: da Silva Luciana Maria , Costa Kátia Aparecida de Pinho , Prado Laís Guerra , Rezende Alexandre Garcia , Severiano Eduardo da Costa , Costa João Victor Campos Pinho , e Silva João Antônio Gonçalves , Costa Adriano Carvalho , Fernandes Patrick Bezerra , Rodrigues Rosane Claúdia , Marques Bruno de Souza , de Morais Breno Furquim , Rodrigues Fabricio
Published: October 2024

Integrated systems are efficient technologies used for diversifying production and promoting agricultural sustainability. The benefits of double intercropping are well-established in research. With advancements in sustainable food production technology, some studies have explored triple intercropping by adding legumes. However, triple intercropping methods must be comprehensively understood. This study aimed to evaluate the production of dry mass, fermentation profile, and nutritive values of silage from maize based intercropping. The experiment was conducted in the field, in Instituto Federal Goiano, Campus Rio Verde, State of Goiás, Brazil, where we assessed silage production and quality in monoculture and intercropping systems, in a randomized block complete design with three replications. We evaluated 8 treatments, consisting of silage from: maize monoculture; Tamani guinea grass monoculture; Quênia guinea grass monoculture; Zuri guinea grass monoculture; Pigeon pea monoculture; maize + Tamani guinea grass + Pigeon pea; maize + Quênia guinea grass + Pigeon pea and maize + Zuri guinea grass + Pigeon pea. The results showed that maize silage intercropped with cultivars of Panicum maximum (Tamani, Quênia and Zuri guinea grasses) and Pigeon pea increased silage mass production by 37.8% and the crude protein content at 25.1% compared with that of the monoculture maize silage. It also improved the fermentation characteristics of silage, reducing 5.1% of pH, 32.5% of buffering capacity, 28.9% of ammoniacal nitrogen in total nitrogen, 15.7% of effluent production, and 20.0% of dry matter losses, compared with those of grass and legume silage in monoculture. Thus, the silage produced by the triple intercropping proved to be an efficient technique for sustainable forage production.
Application of additional dose of N could sustain rice yield and maintain plant nitrogen under elevated ozone (O3) and carbon dioxide (CO2) condition
Authors: Chakrabarti Bidisha , Sharma Sheetal , Mishra Ajay Kumar , Kannojiya Sudha , Kumar V. , Bandyopadhyay S. K. , Bhatia Arti
Published: October 2024

Introduction

Global food security is challenged by the increasing levels of air pollutants like ozone (O3) through their impacts on crop productivity. The present study was conducted to quantify the interactive effect of elevated ozone (O3) and carbon dioxide (CO2), on different rice varieties in northern India.

Methods

An experiment was conducted in Genetic H field, Environment science, IARI for two consecutive years (2020 and 2021) during the kharif season, to quantify the impact of elevated O3 and CO2 interaction on productivity, and plant N in three rice varieties (Pusa basmati 1121, Nagina 22, IR64 Drt1) under different nitrogen (N) management practices. Rice crop was grown in Free Air Ozone-Carbon dioxide Enrichment rings (FAOCE) rings with two levels of O3 (elevated 60 ±10ppb and ambient) and two levels of CO2 (elevated, 550±25 ppm and ambient) concentration and their interaction with two N fertilizer treatments i.e., 100% RDN (recommended dose of N) and 125% RDN.

Results and discussion

Elevated O3 significantly decreased physiological parameters like photosynthesis rate, stomatal conductance and transpiration rate of the crop. Grain yield reduced by 7.2-7.5%, in Pusa Basmati 1121 and from 6.9-9% in IR64 Drt1 varieties in elevated O3 treatment as compared to ambient treatment. Yield reduction in Nagina 22 variety was not significant in elevated O3 treatment. Elevated CO2 concentration of 550 ppm was able to fully compensate the yield loss in Nagina 22 variety and partially compensate (3.9-8.0%) in Pusa Basmati 1121 and IR64 Drt1 varieties. Grain N concentration in rice varieties decreased by 10.8-14.7% during first year and by 7.8-20.6% during second year in elevated O3 plus CO2 interaction treatment than ambient. Grain N uptake also decreased (13.2-17.1% in first year and 4.5-22.8% in second year) in elevated O3 plus CO2 interaction treatment as compared to ambient. Application of additional 25% of recommended dose of N improved grain N concentration, grain N uptake as well as available N of soil as compared to 100% RDN treatment in elevated O3 plus CO2 interaction treatment. Additional 25% N dose could help in sustaining rice productivity and quality under elevated O3 and CO2 condition.
Cannabinoid hyperemesis syndrome: genetic susceptibility to toxic exposure
Authors: Russo Ethan B. , Whiteley Venetia L.
Published: October 2024

Cannabinoid hyperemesis syndrome presents as a complex of symptoms and signs encompassing nausea, vomiting, abdominal pain, and hot water bathing behavior, most typically in a heavy cannabis user. Its presentation is frequently associated with hypothalamic-pituitary-adrenal axis activation with stress and weight loss. Recent investigation has identified five statistically significant mutations in patients distinct from those of frequent cannabis users who lack the symptoms, affecting the TRPV1 receptor, two dopamine genes, the cytochrome P450 2C9 enzyme that metabolizes tetrahydrocannabinol, and the adenosine triphosphate-binding cassette transporter. The syndrome is associated with escalating intake of high potency cannabis, or alternatively, other agonists of the cannabinoid-1 receptor including synthetic cannabinoids. Some patients develop environmental triggers in scents or foods that suggest classical conditioned responses. Various alternative “causes” are addressed and refuted in the text, including exposure to pesticides, neem oil or azadirachtin. Nosological confusion of cannabinoid hyperemesis syndrome has arisen with cyclic vomiting syndrome, whose presentation and pathophysiology are clearly distinct. The possible utilization of non-intoxicating antiemetic cannabis components in cannabis for treatment of cannabinoid hyperemesis syndrome is addressed, along with future research suggestions in relation to its genetic foundation and possible metabolomic signatures.
The Effects of the Combined Application of Biochar and Phosphogypsum on the Physicochemical Properties of Cd-Contaminated Soil and the Yield Quality of Chinese Cabbage
Authors: Mu Liyuan , Zhou Hongyin , Li Ao , Wang Lijuan , Wang Junlei , Sun Sijing , Zhang Naiming , Bao Li
Published: October 2024

Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) is rich in many vitamins and many minerals and is an important green vegetable in people’s daily diet. Soil heavy metal Cd content exceeding safe values has a toxic effect on the growth of vegetables, which seriously affects human health. Biochar can effectively stabilize heavy metals in polluted soil, and phosphogypsum can improve the physical and chemical properties of soil and promote the growth of crops. To explore the remediation effect of biochar combined with different amounts of phosphogypsum on Cd-contaminated soil and the safe production of agricultural products, a pot experiment was conducted with corn straw biochar and harmless phosphogypsum as the test materials, and Chinese cabbage as the test plant. The soil pH; the alkali-hydrolyzed nitrogen content; the available phosphorus, potassium, and Cd content in the soil; the fresh weight of the Chinese cabbage; the Cd content in the edible part of the Chinese cabbage; and the vitamin C, chlorophyll, and soluble sugar contents in the leaves were measured and analyzed. The results showed that among all the treatments, the T3 and T4 treatments were the most effective in enhancing the soil pH and soil available nutrient content. Compared with CK, the T3 and T4 treatments significantly enhanced the soil pH by 0.27 and 0.29 units, respectively, and significantly increased alkali-hydrolyzed nitrogen content by 16.25% and 14.04%, available phosphorus content by 22.98% and 22.87%; and available potassium content by 8.50% and 10.13%. In addition, among all the treatments, the T3 treatment had the best effect on reducing the soil available Cd content and the Cd content in the edible part of the Chinese cabbage, which were significantly reduced by 17.05% and 49.35%, respectively, compared with CK, and the T3 treatment had the best effect on improving the yield and quality of the Chinese cabbage, significantly increasing the fresh weight of the Chinese cabbage by 31.86%, the soluble sugar level by 9.54%, the vitamin C level by 15.38%, and the chlorophyll level by 13.28%, compared with CK. In summary, 3000 kg/hm2 of biochar combined with 3000 kg/hm2 of phosphogypsum can significantly reduce the effectiveness of soil Cd, prevent the transfer of Cd to Chinese cabbage, reduce the ecological risk of Cd, and improve the yield and quality of Chinese cabbage. The results provide a theoretical basis for the safe production of agricultural products in Cd-contaminated soil and promote the recycling of resources for the benefit of the environment.
Risk Assessment of Heavy Metal Accumulation in Cucumber Fruits and Soil in a Greenhouse System with Long-Term Application of Organic Fertilizer and Chemical Fertilizer
Authors: Zhang Yuwei , Tang Shan , Li Yali , Li Ruonan , Huang Shaowen , Wang Hong
Published: October 2024

Combining organic and chemical fertilizers is a sustainable strategy for vegetable production. However, there is limited research concerning the risks associated with heavy metals (HMs) in greenhouse systems with long-term location application. A three-year investigation, conducted from 2021 to 2023, explored a fifteen-year field experiment with combinations of chemical fertilizer (CH), corn straw (SW) and pig manure (PM). Five treatments were evaluated: excessive fertilization (high CH and PM), conventional fertilization (normal CH), organic–inorganic fertilization (3/4CN + 1/4PN, 2/4CN + 2/4PN and 2/4CN + 1/4PN + 1/4SN). This study evaluated the risks associated with heavy metals (HMs) by analyzing and quantifying their concentrations in soil and cucumber fruits, as well as by calculating the bioconcentration factors (BCFs), the geo-accumulation index (Igeo), and both the non-carcinogenic and carcinogenic risks. The results indicated that excessive fertilization (CF) increased the concentrations of Cu and Zn in fruits, as well as the Igeo values of Cu, Zn, and Cd, and the non-carcinogenic Cu risk, while decreasing the BCFs of Cu and Zn. Organic–inorganic fertilization also elevated the Igeo values of Cu and Zn. Redundancy analyses confirmed a positive correlation between the soil concentrations of Cu and Zn and higher levels of available phosphorus contents (48.4%), alongside a lower pH (4.9%). The concentrations of Cu, Zn, and Cd in both soil and cucumber fruits increased linearly with the duration of application and amount of input. Although the combined application of CH with PM or SW did not significantly elevate the non-carcinogenic or carcinogenic risks associated with most heavy metals, the carcinogenic risks of Cd and As emerged as potential risk factors after 15 years of organic–inorganic fertilization. Utilizing a combination of CH with PM and SW as a fertilizer management strategy can effectively address both the control of heavy metal inputs in the facility and the safety and quality of cucumbers.
Anti-Inflammatory and Autophagy Activation Effects of 7-Methylsulfonylheptyl Isothiocyanate Could Suppress Skin Aging: In Vitro Evidence
Authors: Cho Yeong Hee , Park Jung Eun
Published: October 2024

Skin inflammation, characterized by redness, swelling, and discomfort, is exacerbated by oxidative stress, where compounds like 7-methylsulfonylheptyl isothiocyanate (7-MSI) from cruciferous plants exhibit promising antioxidant and anti-inflammatory properties, though their effects on skin aging and underlying mechanisms involving the NLRP3 inflammasome and autophagy are not fully elucidated. NLRP3 is a crucial inflammasome involved in regulating inflammatory responses, and our study addresses its activation and associated physiological effects. Using biochemical assays such as ELISA, RT-qPCR, Western blotting, confocal microscopy, and RNA interference, we evaluated 7-MSI’s impact on cytokine production, protein expression, and genetic regulation in Raw 264.7 and RAW-ASC cells. 7-MSI significantly reduced TNF-α, IL-1β, IL-6, COX-2, and PGE transcription levels in LPS-stimulated Raw 264.7 cells, indicating potent anti-inflammatory effects. It also inhibited NF-κB signaling and NLRP3 inflammasome activity, demonstrating its role in preventing the nuclear translocation of NF-κB and reducing caspase-1 and IL-1β production. In terms of autophagy, 7-MSI enhanced the expression of Beclin-1, LC3, and Atg12 while reducing phospho-mTOR levels, suggesting an activation of autophagy. Moreover, it effectively decreased ROS production induced by LPS. The interaction between autophagy and inflammasome regulation was further confirmed through experiments showing that interference with autophagy-related genes altered the effects of 7-MSI on cytokine production. Collectively, this study demonstrates that 7-MSI promotes autophagy, including ROS removal, and to suppress inflammation, we suggest the potential use of 7-MSI as a skin care and disease treatment agent.