bluuebunny/crossref_metadata_2025 huggingface dataset:
Conocimiento del alumnado de Educación Secundaria de Orihuela (España) sobre el riesgo de inundación
Authors: Zaragoza Sáez Ángela , Morote Seguido Álvaro-Francisco
Published: February 2024

La formación sobre las inundaciones en la población resulta de notable necesidad para incrementar la resiliencia socio-territorial ante este fenómeno. El objetivo de esta investigación es analizar el conocimiento sobre las inundaciones que tiene el alumnado de Educación Secundaria del municipio de Orihuela (España). Metodológicamente, a partir de la realización de un cuestionario en alumnado de 3º curso de Educación Secundaria (n=99) se ha obtenido información sobre su percepción y conocimiento sobre las inundaciones y el riesgo de estos eventos en Orihuela. Los resultados ponen de manifiesto que la mayoría de los/as estudiantes son conscientes de que viven en una zona expuesta que se inunda frecuentemente (63,7%), y reconocen pasar por zonas inundables diariamente. Sin embargo, el alumnado cree que las causas de la inundabilidad son sólo “atmosféricas” (no se tiene en cuenta el factor humano -2,0%-). Con este trabajo se ha avanzado en el conocimiento escolar sobre un fenómeno que cada vez habrá que tener más presente debido a los escenarios futuros de cambio climático, especialmente en aquellos territorios de riesgo como el caso aquí analizado.
Oportunidades laborales para geógrafos: la Formación Profesional de Procesos de Producción Agraria en Andalucía
Authors: Najarro Martín Ulises
Published: February 2024

El presente artículo analiza las oportunidades laborales para geógrafos y geógrafas en la formación profesional de grado medio y superior. Se realiza una breve mención de la situación actual en España y se analiza en profundidad la situación en Andalucía, a través de una revisión documental y del análisis de la normativa actual vigente donde se establecen las titulaciones oficiales para el desempeño de puestos en régimen de interinidad en los cuerpos de enseñanza secundaria y formación profesional. También, se indaga en el análisis del currículo de los respectivos Reales Decretos y la Orden en Andalucía donde se desarrolla los currículos de la formación profesional media y superior. Las conclusiones ponen de manifiesto las posibilidades laborales existentes en Andalucía dentro de la formación profesional para el profesorado de geografía y el gran peso de contenidos geográficos establecidos para cada una de las titulaciones y que dichas enseñanzas son desempeñadas por otras disciplinas excluyendo a la Geografía.
De los teóricos en cómo enseñar la ciudad, a lo que aprenden los estudiantes: la ruta de la geografía urbana escolar en España y Colombia
Authors: Hurtado Beltrán Mario Fernando
Published: February 2024

La enseñanza de la ciudad en la escuela es el tema de interés de este trabajo producto de la investigación doctoral denominada: Las tradiciones de la geografía urbana escolar en España y Colombia, análisis desde la polis, la civitas y el urbs, realizada por el mismo autor. En la misma, se busca identificar qué ocurre con lo que escriben los académicos y los teóricos sobre cómo se debe enseñar la ciudad, y que tanto aprenden en realidad los estudiantes. Para ello, la investigación parte por la revisión documental de los artículos académicos sobre la enseñanza de la ciudad, pasa por la comparación de los lineamientos curriculares nacionales en España y Colombia, la elaboración de textos escolares, su implementación en el aula, las experiencias de profesores innovadores y por último las evidencias de lo que aprenden los estudiantes, con el resultado de seis instituciones educativas, tres en Valencia (España) y tres en Bogotá (Colombia).
Enseñando la complejidad: clima, cambio climático y extremos atmosféricos en Educación Secundaria
Authors: Sánchez Almodóvar Esther , Olcina Cantos Jorge
Published: February 2024

La enseñanza del tiempo y clima, de sus eventos extremos y del proceso de calentamiento climático planetario se ha convertido en un tema prioritario en el contexto actual. La geografía es la disciplina científica que mejor puede presentar de forma didáctica la complejidad de unos procesos atmosféricos que condicionan la vida del ser humano. La aparición de normativas, generales y educativas, que obligan a considerar el cambio climático y sus extremos atmosféricos asociados en la vida cotidiana (territorio, economía, sociedad, educación) permite proponer ideas sobre la manera de transmitir estos procesos en la educación de los diferentes niveles educativos. El trabajo hace repaso a las experiencias didácticas más destacadas que se han desarrollado en España en los últimos años para la enseñanza del clima y el cambio climático. En este sentido se presentan las propuestas didácticas llevadas a cabo desde la Universidad de Alicante (Laboratorio de Climatología) que han resultado exitosas para la transmisión, de forma sencilla, de unos procesos físicos de gran complejidad.
Newly Qualified Teachers and their Preparedness as Geography Teachers in Zambia
Authors: Nakazwe Matilda Kanyampa , Van der Merwe Clinton
Published: February 2024

The study aimed to explore the geography teachers' engagement with the geography content during their undergraduate teacher preparation at the University of Zambia and upon employment in secondary schools. The researcher utilised a case study design to collect data from nine geography teachers trained at the University of Zambia and later employed to teach in the Secondary schools of Zambia. A semi-structured interview guide was employed to collect data from nine geography teachers. Furthermore, the data collected was transcribed, coded and analysed thematically. The study established that most of the geography teachers enjoyed learning either physical geography or human geography. Furthermore, most geography teachers evaluated their teaching to have been slightly better in human than physical geography. Most participants felt adequately prepared to take up geography teaching in schools because they acquired more knowledge than required for secondary teaching. However, a few students who took the subject as their minor needed more human geography content courses.
Motivación laboral y su relación con la productividad de los colaboradores de las agencias de San Borja del banco Interbank 2022
Authors:   , Cashu Ramirez Adriana Steffany
Published: February 2024

Motivar a los empleados hoy en día en sumamente importante para toda organización, porque así se está potenciando la productividad y el posicionamiento en el mercado cambiante, sin embargo, cumplir con las metas aparta en múltiples ocasiones la percepción de valor y cuidado del empleado, descuidando la motivación, empuje y acompañamiento para lograr los objetivos. La investigación buscó analizar si la motivación laboral mantiene relación con la productividad de los colaboradores de las agencias de San Borja del banco Interbank 2022. La metodología que se realizó fue de diseño no experimental, de enfoque cuantitativo, tipo aplicado y alcance correlacional. La muestra se obtuvo de 100 colaboradores, siendo una muestra probabilística aleatoria simple. Para el recojo de datos se elaboró un cuestionario de elaboración propia como instrumento con 25 ítems por ambas variables. Se analizó la data con el coeficiente de correlación Rho de Spearman, hallando correlaciones positivas. La correlación para la hipótesis general fue de (r=0,237), concluyendo que la Motivación ofrecida por la organización no compensa sus necesidades básicas del colaborador y se ve reflejado en la productividad de los colaboradores de las agencias analizadas.
Mejora de visualización de indicadores aplicando inteligencia de negocios y su relación en la toma de decisiones por recaudación tributaria en la Municipalidad Distrital de Lince - 2022
Authors:   , Benavides Redhead Javier Alexander
Published: February 2024

La presente investigación busca evidenciar la mejora de la visualización de indicadores aplicando Inteligencia de Negocios en la municipalidad distrital de Lince. La entidad tiene entre sus diferentes objetivos institucionales el de fortalecer la modernización en los sistemas administrativos y funcionales de la gestión institucional. Esta investigación estuvo planteada como de tipo aplicada, enfocado a un diseño pre experimental y de característica cuantitativa. Se dispuso de una población y muestra de 10 usuarios pertenecientes a la Gerencia de Administración Tributaria, apoyado en la aplicando de la técnica de cuestionario y con el instrumento de tipo encuesta. De los datos recopilados por el instrumento en las pruebas Pre Test y Post Test, se obtuvieron los resultados que nos permitió determinar una relación positiva en relación con la toma de decisiones por recaudación tributarias. Para las pruebas Pre Test se obtuvo una calificación del 50%, en la calificación de nivel bajo en contraposición a las pruebas Post Test, que obtuvo un 50% de nivel alto. La investigación permitió, en su interpretación, el cambio significativo para la toma de decisiones apoyado en indicadores generados por la Inteligencia de Negocios, al evaluar los resultados y encontrar cambios entre los encuestados sobre el tiempo, productividad y presentación de la información en relación con el uso de la Inteligencia de Negocios. Por otro lado, la toma de decisiones se vio afectada positivamente desde la organización dirección, control y evaluación, desde la percepción de los encuestados en los cambios que representó el uso de una herramienta de negocios para la obtención de información capaz de responder a las necesidades de la institución para la toma de decisión, enfocado en la recaudación tributaria.
The study of cases of fixed points of a complex function depending on the inputs of this function
Authors: louiz akram
Published: February 2024

This is a mathematical article that deals with the domain of complex numbers in order to present new results by using a complex function and studying its fixed points.
The aim of this work is to propose new mathematical statements of complex numbers as a probable criterion that can be linked to Landau-Siegel Zeros. Hence, this is a good article to be read by specialists of Calculus and Analysis and even by the beginners of mathematics who want to improve their skills in the field of complex numbers.
Volborthite and Its Composite with g-C3N4 for Oxytetracycline Removal: Their Adsorption and Photocatalysis Properties
Authors: Wee Wei Qing , Sim Lan Ching , Leong Kah Hon , Aziz Azrina Abdul
Published: February 2024

The photocatalytic-adsorption performance of the composites of volborthite (CuVA) and graphitic carbon nitride (g-C3N4) was studied in this work using oxytetracycline (OTC) as model pollutant under LED light irradiation. CuVA at different weight percentage (10, 30, 50), namely C10, C30 and C50 were loaded onto graphitic carbon nitride using wet chemical method. The physical, chemical and optical properties were evaluated via various analytical techniques. Through integrated adsorption-photocatalytic process, no significant photocatalytic reaction occurred in g-C3N4 and the composite even after 4 h of irradiation. The setup was modified such that each run was conducted in the presence and absence of light. Aside from photolysis and g-C3N4, all composites performed better under the presence of light in which CuVA improved the most from ~50% down to ~20% of initial concentration. CuVA performed almost identically (80% removal of OTC) under the presence of light irradiation at ambient temperature (22 °C) and in the dark at 32 °C, confirming that temperature was the contributing factor to the improvement instead of light. CuVA exhibited excellent adsorption capacity of 171 mg/g and adsorption rate of 90% towards the removal of highly concentrated OTC (100 mg/L) under optimized parameters of pH 5.0 and at 42 °C after 3 h of adsorption process. Life cycle assessment revealed that close to 50% of fresh 100 ppm OTC could be removed after 5 cycles without any desorption process.
Effect of Bio-Cellulose + Graphene Graft and Adipose Stem Cell on Axonal Regeneration in Experimental Sciatic Nerve Injury
Authors: Simsek Ismail , Cekic Efecan , Kirazli Ozlem , Unal Semra , Sahin Ali , Harman Ferhat
Published: February 2024

Background
Peripheral nervous system injuries cause catastrophic burdens due to the developing loss of function. The most limiting situation in the treatment of injuries is the low ability of the central nervous system to regenerate itself, and most of these damages are irreversible.
Aim
In this study; we aimed to elucidate the effect of in-vitro application of a scaffold of bio-cellulose and graphene oxide (BC + GO) mixture on the axonal healing potential after experimentally induced sciatic nerve injury in Wistar rats, alone or in combination with mesenchymal stem cells taken from rat viscera.
Materials and Methods
This experimental, prospective, randomized study was conducted on 27 male Wistar rats. They were evaluated into three groups, with nine in each group as Group 1 (autograft group), Group 2 (Graft group), and Group 3 (Graft + SC group). Sciatic nerve function index (SFI), EMG, and histo-pathology evaluation were performed at the end of the 8th week.
Results
There was no significant difference in SFI results within the three groups. In the case of EMG, there was a statistically significant difference among the first-month CMAP results of groups. First-month CMAP results of graft and graft + SC groups were significantly higher than the autograft group (p = 0.0046). During macroscopic observation, graft and graft + SC groups had abundant adhesions around the grafted site. On histo-morphology and immuno-histochemistry, regenerative findings were obtained in all three groups. The autograft group had no inflammatory response, while the graft group had the highest inflammation and degeneration. In the graft + SC group there were less inflammation than second one, which is a finding thought to be related with immunmodulatory effects of MSCs.
Conclusion
BS + GO graft might be useful in the first weeks of regeneration, but this wasn’t sustained in the long-term regenerative findings due to intense inflammatory response and loss of regenerated axonal structures.
Study on fatigue crack growth property of AWJ peened aluminum alloy
Authors: Wang Hui , Lv Zhe , Cui Huanyong , Hou Rongguo , Liu Yuanjian
Published: February 2024

Abrasive waterjet peening is a favorable surface treatment method for improving the fatigue resistance of metal materials. An insight into the fatigue crack growth properties of AWJ peened specimens is meaningful for obtaining better strengthening performance. In present work, a numerical model of AWJ peening was established and experimentally validated for investigating the fatigue crack growth characteristics of Aluminum specimens. The effect of peening and loading conditions on the fatigue performance was also analyzed. The results indicated that the stress intensity factor at the peened region was enhanced and the crack propagation was significantly inhibited by the compressive residual stress. The influence of compressive residual stress on the effective stress factor range is greater under lower external load and higher loading ratio. The fatigue life for reaching the crack length of 40 mm is increased by 37%, 60% and 98% after peening by using the intensity of 0.6 mmA, 0.8 mmA and 1 mmA, respectively.
Half of the unprecedented global soybean production failure in 2012 is attributable to climate change.
Authors: Hamed Raed , Lesk Corey , Shepherd Theodore , Go Henrique M.D. , Garderen Linda van , Hurk Bart van den , Coumou Dim
Published: February 2024

In 2012, soybean crops failed in the three largest producing regions due to spatially compound hot and dry weather across North and South America. Here, we present different impact storylines of the 2012 event by imposing the same seasonally evolving atmospheric circulation in a pre-industrial, present day (+1°C above pre-industrial), and future (+2°C above pre-industrial) climate. While the drought intensity is rather similar under different warming levels, our results show that anthropogenic warming strongly amplifies the impacts of such a large-scale circulation pattern on global soybean production, driven not only by warmer temperatures, but also by stronger heat-moisture interactions. We estimate that 51% (47-55%) of the global soybean production deficit in 2012 is attributable to climate change. Future warming (+2°C above pre-industrial) would further exacerbate production deficits by 58% (46-67%), compared to present-day 2012 conditions. This highlights the increasing intensity of global soybean production shocks with warming requiring urgent adaptation strategies.
Value of X-ray Photography and Magnetic Resonance Imaging in the Diagnosis of Knee Degenerative Osteoarthropathy and the Evaluation of Arthropathy
Authors: Lu Xu , Zhao Yanzhong , Sun Shanhua , Li Hua , Ji Meng
Published: February 2024

Purpose: this work was to explore the diagnostic value and lesion evaluation value of magnetic resonance imaging (MRI) and X-ray in knee joint degenerative osteoarthropathy.
Methods: a total of 92 patients with knee degenerative osteoarthropathy who were admitted to our hospital from March 2021 to September 2022 were included in the study. They were randomly assigned into a control (Ctrl) group (n = 46) and an observation (Obs) group (n = 46) according to the random number table. X-ray films were taken in Ctrl group, and MRI images were taken in Obs group to compare their diagnostic accuracy and evaluation efficiency for arthropathy.
Results: in terms of osseous structural lesions, the detection rates of the two methods were not greatly different (P > 0.05). For meniscal cartilage and peripheral soft tissues, the detection rate of MRI was obviously superior to that of X-ray. The diagnostic sensitivity (95.55%) was obviously higher than that of X-ray (86.04%), and the diagnostic coincidence rate (93.47%) was obviously higher than that of X-ray (80.43%). For the first-stage joint lesions, the detection rates of MRI (55% and 11/20) were drastically superior to those of X-ray (35% and 7/20), while for the second-stage joint lesions, the detection rates of MRI (66.66% and 14/21) were higher than those of X-ray (45.4% and 10/21) (P< 0.05).
Conclusion: the diagnostic coincidence rate and detection rate of knee degenerative osteoarthropathy with MRI were superior to those with plain X-ray film, which is helpful for the early diagnosis of the disease and worthy of clinical promotion and adoption.
Rethinking hormonal contraception in breast cancer survivors
Authors: Pachnicki Jan Pawel , Rebelatto Carmen Lucia , Ribas Carmen Australia , Fracaro Leticia , de Aguiar Alessandra , Silva Eduardo
Published: February 2024

Approximately 7% of women with breast cancer have less than 40 years at diagnosis. Despite of more aggressive characteristics of these tumors, earlier diagnosis and better treatments had increased the number of cancer survivors, and topics such as hormonal treatments, previously unimportant, have now to be discussed. As the concentration of the progestin in breast tissue of patients using Levonorgestrel intrauterine system is very low, there may be a safe alternative for this population. Here we show that low-dose Levonorgestrel did not increase proliferation of breast cells suggesting that, in well-defined situations, some exogenous hormones might be used in breast cancer survivors, thus improving their quality of life. We found in controlled in vitro assays that two mammary cell lines, MCF10A and MCF7, did not show cellular proliferation when exposed to the concentration of 0.01μM Levonorgestrel, even with positive controls of proliferation carried out with Estradiol showing significant difference in both strains. Our results demonstrate that this progestin alone does not seems to be the cause of breast cancer, and our assay may be a “restarting” point for more sophisticated in vitro models that can really explain what is safe, and what is not, for breast cancer survivors.
Uncarboxylated Osteocalcin Inhibits De Novo Lipogenesis and Promotes Fatty Acid Oxidation via SIRT1 to Alleviate Hepatocyte Lipid Accumulation
Authors: Chen Lei , Zhang Miao , Xu Jiaojiao , Yang Jianhong
Published: February 2024

Background Non-alcoholic fatty liver disease (NAFLD) is a disease characterized by the hepatic lipids accumulation resulting from genetic susceptibility and metabolic dysfunction. Uncarboxylated osteocalcin (GluOC) is a protein that is synthesized by osteoblasts which performs a vital function in the management of energy balance. Previous studies have shown that GluOC is beneficial for lipid and glucose metabolism in KKAy mice induced fatty liver. GluOC effectively ameliorates hyperglycemia, fatty liver, and hyperlipidemia. Furthermore, it regulates stearyl-coenzyme A desaturase 1 (SCD1) expression through AMP-activated protein kinase (AMPK), which alleviates hepatocyte lipid accumulation. However, the underlying mechanisms by which GluOC alleviates hepatocyte lipid accumulation need further elucidation. Methods In this study, the NCTC 1469 cells induced by oleic acid (OA) and palmitic acid (PA) were used in the establishment of the NAFLD cell model. Triglyceride (TG) kits and BODIPY 493/503 staining were employed to measure the levels of hepatocyte lipid accumulation. Immunoprecipitation, western blotting, and real-time PCR analyzed the expression of protein and mRNA in the fatty acid oxidation (FAO) and de novo lipogenesis (DNL) pathways. Results The results indicated that increasing concentrations of GluOC resulted in reduced intracellular lipid accumulation and triglyceride levels. GluOC promoted sirtuin1 (SIRT1) expression, inhibited acetyl-CoA carboxylase (ACC) and fatty acid synthetase (FASN) expression, promoted medium-chain acyl-CoA dehydrogenase (MCAD) and long-chain acyl-CoA dehydrogenase (LCAD) expression. In addition, GluOC activated AMPK phosphorylation and peroxisome proliferator activated receptor γ coactivator-1 α (PGC-1α) deacetylation. si-SIRT1 attenuated the above effects of GluOC, resulting in hepatocyte lipid accumulation. Conclusion GluOC inhibited DNL via SIRT1-AMPK and promoted FAO via SIRT1-PGC-1α to alleviate lipid accumulation in hepatocytes. This provides new insights for further research in NAFLD.
Chronic bacterial prostatitis treatment using quadruple regimen therapy: a preliminary prospective observational study
Authors: Hameed Farooq , Anwaar Adeel , mannan Abdul , Hashmi Saadat , Zahoor Muhammad Imran , Anwar Mamoona , Anwar Maria , Saleem Abdullah
Published: February 2024

Background
This study explored the data collected from 27 patients who underwent a new treatment combination of drugs using quadruple regimen therapy with tadafil 5 mg, N-acetylcysteine 200 mg, diclofenac sodium, and a targeted antibiotic for chronic bacterial prostatitis treatment.
Methods
Twenty-seven Pakistani patients underwent a three-glass test. Those who tested positive received quadruple therapy, while negative cases were treated with triple therapy minus antibiotics, using a gradual tapering off technique for a duration of 20 weeks, and followed for 1 year to observe the recurrence of the disease. Patients were evaluated for international prostatic score, improvement in quality of life, uroflowmeteric studies, improvement in symptoms, and disease recurrence. Chi-square test was used to analyze the data, and p value < 0.05 was considered significant.
Results
This study yielded favorable results with significant improvements in symptoms, IPSS score, quality of life, urinary flow rate, and post-micturition residual volume (p < 0.05). Of the 19 participants with positive three-glass tests, 14/19 (73%) saw a complete resolution of symptoms; however, three (26%) had a reemergence six months later. The remaining eight patients (29%) who tested negative did not show the same level of effectiveness as the positive cases, but still observed improvements in quality of life and IPPS score (P < 0.05). However, their symptoms were not fully resolved.
Conclusions
Our study resulted in significant improvement in the patients’ IPSS score, disease symptoms and quality of life after 20 weeks of therapy, with five visits each. All medications were gradually tapered.
Hematological alterations associated with the SNV rs10974944, part of the 46/1 haplotype, in patients from the Brazilian Amazon with BCR::ABL1-negative myeloproliferative neoplasms
Authors: Paes Jhemerson F. , Torres Dania G. , Aquino Deborah C. , Alves Emanuela V. B. , Mesquita Erycka A. , Sousa Miliane A. , Fraiji Nelson Abrahim , Passos Leny N. M. , Abreu Rosângela S. , Silva George A. V. , Tarragô Andréa M. , Mourão Lucivana P. de Souza
Published: February 2024

BCR::ABL1-negative myeloproliferative neoplasms are hematopoietic disorders characterized by panmyelosis. JAK2 V617F is a frequent variant in these diseases and often occurs in the 46/1 haplotype. The G allele of rs10974944 has been shown to be associated with this variant, specifically its acquisition, correlations with familial cases, and laboratory alterations. This study evaluated the association between the 46/1 haplotype of JAK2 in patients with myeloproliferative neoplasms in a population from the Brazilian Amazon. Clinical, laboratory and molecular sequencing analyses were considered. Carriers of the G allele of rs10974944 with polycythemia vera showed an increase in mean corpuscular volume and mean corpuscular hemoglobin, while in those with essential thrombocythemia, there was an elevation in red blood cells, hematocrit, and hemoglobin. Associations were observed between rs10974944and the JAK2 V617F, in which the G allele (OR: 3.47; p < 0.0001), CG genotype (OR: 8.4; p = 0.002), and GG genotype (OR: 4.1; p = 0.002) were associated with JAK2 V617F+ and an increase in variant allele frequency (GG: OR 13.1; p = 0.004; G: OR: 6.0; p = 0.0002). These results suggest an association between rs10974944 (G) and a status for JAK2 V617F, JAK2 V617F+_VAF ≥50%, and laboratory alterations in the erythroid lineage.
Simulation of interfacial debonding in hollow particle-reinforced composites with VCFEM
Authors: Wang Zhiyi , Zhang Rui
Published: February 2024

The addition of hollow glass miscrosphere into composites is a method to improve mechanical properties. However, the interfacial debonding of hollow miscrosphere inevitably causes a decrease in the mechanical properties of the material, which ultimately leads to the failure of the composites. In the numerical simulation of such hollow particle-reinforced composites, the ordinary displacement finite element requires a large number of meshes, which undoubtedly greatly increases the computational cost. In this paper, a new VCFEM is proposed to solve this problem by establishing a two-dimensional Voronoi cell finite element model, deriving the residual energy generalized function of hollow particle-reinforced composites, and calculating the interface debonding. The simulation results are compared with the commercial software MARC, ABAQUS to verify the effectiveness of this VCFEM. The results show that this VCFEM greatly improves the computational efficiency while ensuring the accuracy. Based on this model, this paper also investigates the effect of the generation of interfacial debonding on the overall structure and the effect of different wall thickness of hollow particles on the damage of element debonding.
Implementation of evidence in oral care for adult mechanically ventilated patients with orotracheal intubation using the framework of evidence-based continuous quality improvement: A quality improvement project
Authors: Liu Yanan , Sun Xiaofei , Chen Cancan , Zang Shuting , Zhang Junmei , Zhang Juan , Ren Ying , Wu Hao
Published: February 2024

Background Effective oral care can eliminate bacteria from the oral cavity, enhance oral hygiene, and decrease the occurrence of ventilator-associated pneumonia in mechanically ventilated patients with orotracheal intubation. However, proper implementation of oral care for mechanically ventilated adult patients undergoing orotracheal intubation has not been achieved in clinical practice. The aim was to examine the effectiveness of implementing the evidence in oral care for adult mechanically ventilated patients with orotracheal intubation using the framework of evidence-based continued quality improvement and to take actions to improve nursing quality.Methods Pre- and post-implementation studies were carried out employing a framework of evidence-based continuous quality improvement at the comprehensive intensive care unit of Henan Provincial People’s Hospital in Zhengzhou, China from January to December 2022. A baseline survey was conducted to analyse barriers and facilitators to evidence implementation, informing the development of support strategies such as training nursing staff and creating resources, system flow improvements, and tools. After the evidence implementation, changes in nurses, patients, and the system were assessed. The statistical analysis of the data was performed using SPSS 26.0.Results Nurses' knowledge scores and compliance with evidence significantly improved. The modified Beck Oral Assessment Score decreased from (16.28 ± 1.02) to (8.52 ± 0.45). The detection rate of oral sputum pathogens decreased from 50.0–24.0%. Standardized oral care and workflows for mechanically ventilated adult patients undergoing orotracheal intubation were successfully established.Conclusion The framework of evidence-based continuous quality improvement facilitated the successful implementation of evidence in oral care for adult mechanically ventilated patients with orotracheal intubation. This resulted in improved knowledge among nurses and enhanced oral hygiene for patients, ultimately contributing to the ongoing improvement of nursing quality.
Impact of Timing on Lower Extremity Amputations in Blunt Polytrauma : A Retrospective Analysis of Clinical Outcomes and Risk Factors
Authors: Kim Jinjoo , Choi Wanseon , Cho Wontae , Moon Jonghwan
Published: February 2024

Delayed amputation after failed limb salvage can lead to negative clinical and functional outcomes due to complications, including re-amputation. This study aimed to compare clinical outcomes and identify risk factors according to the timing of amputation. A retrospective review of managed lower-extremity injuries was conducted between January 2016 and December 2022 at a level 1 trauma center. Outcomes were compared between the early amputation and the delayed amputation groups (within and beyond 48 h after the time of injury, respectively). The primary outcome of interest was that the risk factors changed to a more proximal amputation level according to the timing of amputation. The secondary outcomes included trauma-related complications and clinical outcomes. The incidence of trauma-related complications was more common in the delayed amputation group with no significant differences in 30-day mortality. Delayed amputation and stump site deep surgical infection were associated with higher odds of changing to a more proximal level than initially predicted. The overall hospital stay was significantly longer in the delayed amputation group. Although patients and clinicians prefer the limb salvage strategy, delayed amputation could be a risk factor for shorter limb stumps due to re-amputation, and patients may experience more trauma-related complications and prolonged hospitalization.
Farmers’ knowledge and attitude towards the use of low-cost stationary brush for dairy cows
Authors: Nascimento Fernanda Gatti de Oliveira , Brito Amanda Aparecida , Bóscollo Paola Perez , Brettas Patrícia Kelly de Moraes , França André Madeira Silveira , De-Sousa Karolini Tenffen , Faria Priscila Neves , Guimarães Ednaldo Carvalho , Nascimento Mara Regina Bueno de Mattos
Published: February 2024

The aim was to assess farmers’ knowledge of herd behaviour, grooming-related injuries, stress reduction, and familiarity with a brush device, and their attitude in adopting a low-cost stationary brush. Farmers from Southeast and Midwest of Brazil participated in an online survey. The questionnaire was organized into sections: first about the profile of farmers, second focused on the description of milk production system, and third explore farmers' knowledge and attitude towards brush devices. The data were analyzed using descriptive statistics, and a multiple logistic regression model estimated the odds ratio. Farmers with a higher level of milking system technification were found to have 2.85 times greater odds of recognizing the need to reduce stress. Farmers interested in a low-cost brush were found to have 10.48 times greater odds of recognizing the need for stress-reducing improvements. Those who believe that the use of a brush improves milk production and cow welfare have 14.15 times higher odds of installing the low-cost brush. Most farmers reported familiarity with cow brushes. Moreover, farmers recognize the use of brushes as a beneficial strategy for reducing stress, improving milk production, and enhancing animal welfare, Consequently, they express willingness to install a low-cost stationary brush for dairy cows.
Validity of annealed approximation in a high-dimensional system
Authors: Um Jaegon , Hong Hyunsuk , Park Hyunggyu
Published: February 2024

This study investigates the suitability of the annealed approximation in high-dimensional systems characterized by dense networks with quenched link disorder, employing models of coupled oscillators. We demonstrate that dynamic equations governing dense-network systems converge to those of the complete-graph version in the thermodynamic limit, where link disorder fluctuations vanish entirely. Consequently, the annealed-network systems, where fluctuations are attenuated, also exhibit the same dynamic behavior in the thermodynamic limit. However, a significant discrepancy arises in the incoherent (disordered) phase wherein the finite-size behavior becomes critical in determining the steady-state pattern. To explicitly elucidate this discrepancy, we focus on identical oscillators subject to competitive attractive and repulsive couplings. In the incoherent phase of dense networks, we observe the manifestation of random irregular states. In contrast, the annealed approximation yields a symmetric (regular) incoherent state where two oppositely coherent clusters of oscillators coexist, accompanied by the vanishing order parameter. Our findings imply that the annealed approximation should be employed with caution even in dense-network systems, particularly in the disordered phase.
Integrative analysis of transcriptome and metabolome profiling reveal coloration molecular mechanisms in four rapeseed petals with different color
Authors: Zheng Tao , Chen Qiao , Chen Mengjiao , Zeng Haitao , Xue Yan , Tang Qi , Xu Hao , Li Ying
Published: February 2024

Background
The colorful rapeseed petals have specific phenotype and excellent ornamental value. However, the coloration mechanism of rapeseed petals are poorly understood. In this study, the integrative metabolome and transcriptome analysis of white, yellow, dark purple, and dark red rapeseed petals were analyzed to elucidate the mechanisms regulating petal pigmentation.
Results
The metabolome analysis results revealed that chalcones compounds, tamarixetin-3-O-glucoside-7-O-rhamnoside and 4'-hydroxy-2,4,6-trimethoxydihydrochalcone diaplayed a higher accumulation in the yellow color of rape petals, acylated anthocyanins, cyanidin-3-O- (6"-O-caffeoyl) sophoroside-7-O-glucoside and cyanidin-3-O-feruloylglucoside-5-O-glucoside were the main pigments that caused the dark purple color of rapeseed petals, and glycosylated anthocyanins, peonidin-3-O-glucoside were the main coloring substances in the dark red rape petals. KEGG and qRT-PCR results suggested that the higher expression of CHS lead to the accumulation of 4'-hydroxy-2,4,6-trimethoxydihydrochalcone in yellow petals. ANS1, CCoAAT2 and CCoAOMT were responsible for regulating the biosynthesis of cyanidin-3-O- (6"-O-caffeoyl) sophoroside-7-O-glucoside and cyanidin-3-O-feruloylglucoside-5-O-glucoside in dark purple petals. ANS1, UGT, MT1, and MT2 co-expressed to regulate peonidin-3-O-glucoside content in dark red petals. The differential expression of CHS, ANS, UGT, CCoAOMT, CCoAAT2, and MT2 was contributed to the yellow, dark purple and dark red formation of rapeseed flowers. Through weighted gene co-expression network analysis, it was found that five modules were highly correlated with flavonoids accumulation, in which five candidate transcription factors MYB12, MYB122, MYB114, bHLH21, and bHLH96 related to flavonoids biosynthesis were identified.
Conclusion
A genetic and metabolic regulatory network of flavonoid biosynthesis in rapeseed petals with different color was proposed, according to the those differential flavonoids and the candidate genes screened. In summary, this study elucidated the molecular mechanism of coloration formation in rapeseed petal for comprehensive utilization.
Mining and analysis of adverse event signals of Mometasone furoate based on real-world data from the FAERS database
Authors: Zou Zhixin , Long Ziyi , Zhang Qidi , Chen Jianjun
Published: February 2024

Background
Mometasone furoate (MF) is a steroidal active pharmaceutical ingredient (API) that has been approved for the treatment of atopic dermatitis, eczema, allergic rhinitis, and chronic rhinosinusitis by the FDA. The aim of the present study was to assess real-world MF-related adverse events (AEs) through data mining of the US Food and Drug Administration Adverse Event Reporting System (FAERS).
Methods
Disproportionality analyses, including the reporting odds ratio (ROR), the proportional reporting ratio (PRR), the Bayesian confidence propagation neural network (BCPNN) and the multi-item gamma Poisson shrinker (MGPS) algorithms, were employed to quantify the signals of MF-associated AEs.
Results
Out of 49,659,292 reports collected from the FAERS database, 11,510 reports of MF-associated AEs were identified. A total of 185 significant preferred terms (PTs) conforming to the four algorithms were retained. The most common AEs included epistaxis, nasal dryness, burning, stinging, blood-tinged secretions, pharyngeal inflammation, skin weeping and pruritis allergic reactions, which corresponded to those reported in the specification and clinical trials. Unexpected significant AEs, such as chorioretinopathy, retinal edema, ageusia, anosmia, otorrhea, ear pruritus, auricular swelling and limb reduction defects, might also occur.
Conclusion
The results of our study were consistent with clinical observations, and we also found potential new and unexpected AEs associated with MF. Prospective clinical studies are needed to confirm these results and determine their relationship. Our results could provide valuable evidence for further safety studies of MF.
The impact of time perception on consumer’s intertemporal choices: a study on self-improvement and self-enhancement products
Authors: Hou Min , Yan Linlin , Gu Chunmei
Published: February 2024

Consumer decisions often have both short- and long-term consequences, making Subjective Time Perception an important variable in intertemporal choice research. However, limited studies have explored the relationship between the subjective time perception and intertemporal choices in specific product contexts. To gain a deeper understanding of consumer behavior, it is crucial to examine how subjective time perception (STP) impacts the decision-making process. This study focuses on subjective time perception and its effect on intertemporal choices, while also considering the moderating role of product type (self-improvement vs. self-enhancement). Study 1 shows that individuals with a shorter sense of STP are more inclined to choose delayed rewards. Study 2 further confirms that consumers make different intertemporal choices based on the product type. For self-improvement products, individuals with a shorter STP are more likely to choose delayed rewards, whereas those with a longer STP are more inclined to choose immediate rewards. On the other hand, for self-enhancement products, there was no difference in intertemporal choices. Lastly, Study 3 reveals that the way individuals perceive time plays a significant moderating role in how product type influences intertemporal choices. The research findings confirm previous evidence on the important role of time perception in intertemporal choices under different product contexts.
Significant efficacy of conversion therapy for advanced hepatocellular carcinoma: a case report
Authors: Fang Cheng , Zheng Rujun , Guo Qinxi , Li Bo , Su Song
Published: February 2024

Hepatocellular carcinoma (HCC) is the sixth most common cancer and the third leading cause of cancer-related mortality worldwide [1]. This study aimed to report a case with advanced-stage HCC who underwent transhepatic arterial chemotherapy and embolization combined with anlotinib, sintilimab, and radiotherapy. He successfully received conversion therapy to get tumor downstaging and finally had an opportunity to undergo a right lobe hepatectomy. The postoperative pathological examination confirmed a pathologic complete response, and the level of serum alpha-fetoprotein decreased from > 1000 to 11.33 ng/mL. The follow-up to date revealed that the progression-free survival time was more than 13 months. It indicated that combined therapy might be a superior choice for the conversion therapy of patients with advanced-stage HCC.
Biofilm-disrupting effects of phage endolysins LysAm24, LysAp22, LysECD7, and LysSi3: breakdown the matrix
Authors: Lendel Anastasiya M. , Antonova Nataliia P. , Grigoriev Igor V. , Usachev Evgeny V. , Gushchin Vladimir A. , Vasina Daria V.
Published: February 2024

The ability of most opportunistic bacteria to form biofilms, coupled with antimicrobial resistance, hinder the efforts to control widespread infections, resulting in high risks of negative outcomes and economic costs. Endolysins are promising compounds that efficiently combat bacteria, including multidrug-resistant strains and biofilms, without the subsequent emergence of endolysin-resistant genotypes. However, the details of antibiofilm effects of these enzymes are poorly understood. To elucidate the interactions of bacteriophage endolysins LysAm24, LysAp22, LysECD7, and LysSi3 with bacterial films formed by Gram-negative species, we estimated their composition and assessed the endolysins’ effects on the most abundant exopolymers in vitro. The obtained data suggests a pronounced efficiency of these lysins against biofilms with high (Klebsiella pneumoniae) and low (Acinetobacter baumannii) matrix contents, or dual-species biofilms, resulting in at least a 2-fold loss of the biomass. These peptidoglycan hydrolases interacted diversely with protective compounds of biofilms such as extracellular DNA and polyanionic carbohydrates, indicating a spectrum of biofilm-disrupting effects for bacteriolytic phage enzymes. Specifically, we detected disruption of acid exopolysaccharides by LysAp22, strong DNA-binding capacity of LysAm24, both of these interactions for LysECD7, and neither of them for LysSi3.
Virome characterization of field-collected rodents in suburban Beijing reveals a spectrum of emerging pathogens
Authors: Hu Zhen-Yu , Tang Fang , Zhang Jing-Tao , Zhang Yun-Fa , Si Guang-Qian , Fu Bo-Kang , Wang Gang , Li Shuang , Zhang Lei , Peng Cong , Ma Xiao-Fang , Liu Yan-Tao , Bao Peng-Tao , Zhang Xiao-Ai , Liu Wei , Zhang Mei-Qi
Published: February 2024

Background
Rodents serve as natural reservoirs and transmission hosts for numerous zoonotic viruses, which can cause a range of animal and human diseases, posing significant public health concerns. Analyzing the viral diversity harbored by rodents is crucial for early warnings of emerging infectious diseases.
Results
By conducting meta-transcriptomic sequencing on spleen samples obtained from 432 wild rodents across three habitats, we characterized the high-depth RNA virome of wild rodents representing 9 species of two prominent mammalian families (Cricetidae and Muridae) in suburban Beijing. The composition of virome varied significantly at the virus family level among the nine rodent species and three types of natural habitats. A total of 142 viral species associated with vertebrates (n = 133) and invertebrates (n = 9) were identified from 26 families, including 75 novel viruses and 67 known viruses, thereby substantially expanding our knowledge about the diversity of rodent virome. Among these, twenty-five viruses were classified as high-risk, including 8 zoonotic viruses and 17 spillover-risk viruses. Additionally, nine previously unreported viruses were discovered for the first time in China. Furthermore, thirty-three viruses exhibited species transmission potential and some had evolutionary significance.
Conclusions
These findings enhance our understanding of rodent virome in Suburban Beijing and suggest that there is vast array of undiscovered viruses within these rodent species in China. Understanding the composition of rodent virome might provide insights into the potential risk of zoonotic spillover to humans.
suPAR is a potential biomarker of stage III-IV, grade C periodontitis through the impact of post-radiotherapy on head and neck cancer patients
Authors: Al-Kubaisi Ahmed A. , Ghazi Maysam Abdulrahman , Majeed Nisreen Salah , Aldelaimi Ekram R. , Enezei Hamid H.
Published: February 2024

Background: The urokinase-type plasminogen activator receptor (uPAR) plays an essential function in leukocytes and endothelial homeostasis and, therefore, in the development of chronic periodontitis.
Methods: The study enrolled 150 participants, including 50 (CP+HNC post-RT) patients, 50 (CP) without HNC patients, and 50 healthy controls. Clinical Attachment Loss (CAL), Probing Pocket Depth (PPD), Plaque Index (PI), and Gingival Bleeding Index (GBI) were recorded. An enzyme-linked immunosorbent assay (ELISA) was constructed to quantify serum (suPAR) levels.
Results: Stage and grade of periodontitis were stage III-IV, grade C in patients (CP+HNC post-RT), stage I-III, grade A/B in patients (CP without HNC), and absent in (healthy). Chronic periodontitis with HNC post-RT patients presented a significantly higher proportion of suPAR levels (506.7 pg/ml) compared to chronic periodontitis without HNC and healthy controls (423.08 pg/ml and 255.9 pg/ml), respectively. A significant positive correlation was found between serum suPAR levels and CAL, PPD, PI, and GBI in the periodontal disease groups. ROC results of suPAR (AUC=0.976 for CP+HNC post-RT, AUC=0.872 for CP without HNC). Hyposalivation was decreased in patients (CP+HNC post-RT; 0.15 [0.11-0.23] ml/min, P=0.001) and (CP without HNC; 0.30 [0.25-0.41] ml/min, P=0.001), compared to healthy controls; 0.35 [0.28-0.54] ml/min, P=0.001).
Conclusion: The study showed a significant elevation in serum suPAR levels in CP+HNC post-RT patients compared to the CP without HNC and control groups.
Polyvinyl chloride promoted the dissemination of antibiotic resistance genes in Chinese soil: A metagenomic viewpoint
Authors: Zhao Shuwen , Zhang Qianru , Huang Qilan , Zhang Chuchen
Published: February 2024

Microplastics (MPs) are becoming progressively widespread in the surrounding and are regarded as vectors of antibiotic resistance genes (ARGs). Soils of various properties carry large amounts of microplastics and ARGs. However, a thorough research evaluating the impact of different regions of China in evolving antibiotic resistome in soil MPs is insufficient. Here, we engaged a massive investigation by putting Polyvinyl chloride microplastics (PVC) into soil in 20 provinces of China which have different physical and chemical properties. The results showed that PVC could significantly affect soil bacterial community structure and ARGs abundance. Structural equation models showed that the addition of PVC changed the characteristics of the soil, which in turn influenced the bacterial community in the soil (which included ARG-containing bacterial hosts) and, ultimately, the relative abundance of ARGs. This work improves our comprehension of the effects of microplastics on the proliferation and hosts of ARGs in various soil environments, and it serves as a crucial reference for future plastic consumption and disposal.