bluuebunny/crossref_metadata_2025 huggingface dataset:
Hierarchical Folding of Sub-domais coded by Exons and Molten Grobule State of Retinol Binding Protein
Authors: Nosaka Michiko
Published: May 2022

Understanding the folding dynamics of tertiary structures is important for protein design and engineering. However, something new information is needed for the further progress of protein research. Comparison between the predicted structures for the partial peptide sequence and the corresponding part of the X-ray structure seemed to show some clue to the information. Here, we uncovered another structural hierarchy of retinol binding protein; most subdomains coded in exons fold independently with extended C-terminals and the latter segment interact with the former folded subdomain to form the final structure. Our result was discussed with Exon Junction Complex and the tunnel structure of ribosome. Furthermore, the structural variability of the subdomain coded in the third exon was suggested to have a unique characteristic. Molecular dynamics of this protein with SDS micelle showed a possible change at the latter half of the third exon, whose secondary structure ratio is consistent with published results of the CD spectrum. Therefore, this conformational change is responsible for the molten globule state of the protein for releasing its ligand to a target cell. Although our result and discussion must be confirmed in another proteins and by experimental study, they should be useful for protein research.
Integrated bioinformatics analysis reveals significant genes associated with cryptorchidism (CO)
Authors: Hu Liren , Fan Xianming , Lin Jianfeng , Liang Fulv , Tu Jianping , Guo Zhaojian
Published: May 2022

Objective: we performed a comprehensive bioinformatics analysis to identify potential genes and signaling pathways associated with cryptorchidism.Materials and Methods: This study aimed to identify genes significantly associated with cryptorchidism (CO), between the LE and ORL gubernaculum groups at GD17 and GD19 stages, using a GSE57924 dataset. We identified other DEGs which were changed to up-regulation or down-regulation between the groups by adjusting the cut-off (|log2 fold change (FC)| ≥0.5 and adjusted p <0.05). We subjected the identified DEGs to gene ontology (GO) and pathway enrichment analyses, using an online database DAVID, then employed Cytoscape v3.7.1 to construct a protein-protein interaction network.Results: We detected functional hub genes using MCODE. Overall, we identified 147 DEGs between the LE gubernaculum group and ORL gubernaculum group, of which 33 genes were up-regulated (21 genes always down-regulated and 13 genes from down-regulated in GD17 to up-regulated in GD19) while 96 was down-regulated (27 genes always down-regulated and 69 genes from up-regulated in GD17 to down-regulated in GD19). Gene Ontology (GO) revealed that cell adhesion and extracellular matrix were the most abundant biological processes and Cellular Components, whereas the ECM-receptor interaction was the most significant pathway. Analysis of hub genes in the PPI network revealed that 7 genes including Igfbp5, Cyr61, Lamb1, Lamc1, Cp, Fn1, and Ltbp1, were significantly associated with CO. Conclusions: we successfully identified 7 significant markers that provide valuable insights into the molecular mechanism of cryptorchidism. Of note, one of the genes (Fn1) was verified significant differential expression gene in human testicular cancer.
Mobilization of hematopoietic stem cells with lenograstim in multiple myeloma patients: prospective multicenter observational study (KMM122)
Authors: Jung Eun Hee , Byun Ja Min , Shin Dong-Yeop , Do Young Rok , Jo Jae-Cheol , Lee Sang Min , Yoon Sung-Soo
Published: May 2022

Current guidelines recommend using filgrastim or tbo-filgrastim to mobilize hematopoietic progenitor cells in an autologous setting. However, previous studies have suggested other forms of granulocyte colony-stimulating factor (G-CSF) are equally efficacious, possibly with fewer leukaphereses required. Thus, we prospectively studied the efficacy of lenograstim, a glycosylated recombinant form of G-CSF, in multiple myeloma (MM). From November 2011 to January 2020, 98 MM patients undergoing autologous stem cell transplant (ASCT) from 5 academic centers in Korea were enrolled. Donors were mobilized with subcutaneous lenograstim (Neutrogin®) with fixed doses of 10 ug/kg for four days. Most of patients (N = 90, 91.8%) achieved at least the targets of 2 x 106 CD34+ cells/kg and more than half of patients (N = 57, 58.2%) reached target of 5 x 106 CD34+ cells/kg. The mobilization failure rate was 8.2% (N = 8). The median number of CD34+cell/kg using G-CSF only was 5.25 x 106/kg (range 0.49-13.47). There were no grade 3 or 4 adverse events during mobilization. BSA at mobilization and platelet transfusion were factor associated with CD34+ collection. Most patients achieved neutrophil (N = 93, 98.9%) and (N = 89, 94.7%) engraftment. Lenograstim can safely and effectively mobilize stem cells in MM autologous settings.
Does the level of inferior mesenteric artery ligation affect short-term and long-term outcomes of patients with sigmoid colon cancer or rectal cancer-a single-center retrospective study?
Authors: Wang Yawei , Wang Yan , Zou Liaonan , Deng Lingna , Wu Tianchong , Liu Linsen , Jiang Jiling , An Tailai
Published: May 2022

Background: For patients with sigmoid colon cancer or rectal cancer, a definite consensus in terms of the optimal level ligating the inferior mesenteric artery (IMA) has not been reached. We performed this retrospective study to determine whether the ligation level significantly affected short-term and long-term outcomes of patients with sigmoid colon cancer or rectal cancer after curative laparoscopic surgery. Methods: Medical records of patients with sigmoid colon cancer or rectal cancer who had undergone curative laparoscopic surgery (High tie or low tie of IMA) at The Department of Gastrointestinal Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine were reviewed. Then hie tie group (HTG) was compared with low tie group (LTG) in terms of short-term and long-term outcomes. Results: Between January 2008 and December 2014, a total of 590 patients with sigmoid colon cancer or rectal cancer underwent curative laparoscopic surgery at our center. No significance between two groups regarding baseline characteristics. Patients in the HTG had a significantly higher risk of anastomotic fistula than LTG (21/283 VS 11/307, P=0.040). However, two groups were not significantly different from each other regarding operative time (P=0.075), blood transfusion (P=1.000), estimated blood loss (P=0.239), 30-day mortality (P=1.000), ICU stay (P=0.674), postoperative hospital stay(days) (P=0.636), bowel obstruction (P=0.659), ileus (P=0.637), urinary dysfunction (P=1.000), surgical site infection (SSI) (P=0.121), number of retrieved lymph nodes (P=0.501) and number of metastatic lymph nodes (P=0.131). Subsequently, it was revealed that level of IMA ligation did not significantly influence overall survival (P=0.474) and relapse-free survival (P=0.722). Additionally, it was revealed that ligation level did not significantly affect overall survival (OS) (P=0.460) and relapse-free survival (RFS) (P=0.979) of patients with stage I cancer, which was observed among patients with stage II or stage III cancer. Ultimately, ligation level was not an independent predictive factor for either OS or RFS. Conclusions: HT resulted in a significantly higher incidence of anastomotic fistula. Level of IMA ligation did not significantly affect long-term outcomes of patients with sigmoid colon cancer or rectal cancer after curative laparoscopic surgery.
The correlation analysis between childhood behavioral disturbance and maternal alexithymia: an observational study
Authors: Lin Yu-Chen , Lee Wei-Yang
Published: May 2022

Background
From the perspective of children's psychological development and psychoanalysis, the primary caregiver's response to children's behavior has a considerable impact on children's emotional and behavioral development. If caregivers lack the ability to empathize, difficulties in detecting and responding to children's emotions, which may lead to children's emotional and behavioral disorders. The purpose of this study is to preliminarily explore the correlation between children's behavioral and emotional disorders and the mother's inability to express emotions.
Method
We collected a total of 78 cases from outpatient clinics, aged between 3–13 years old. The Taiwan version of the " Toronto Alexithymia Scale" and "Children's Behavior Checklist" questionnaires were used as research tools, which were filled out by the mothers themselves. We use Spearman correlation tool for follow-up analysis.
Results
The factor 1, 2 and 3 of TAS are positively correlated with somatic complaint subscale of CBCL. (F2: p < 0.01; F1 and F3:P < 0.05); The factor 1 of TAS is positively correlated with internal subscale of CBCL(p < 0.05) and withdrawn subscale of CBCL(p < 0.05). We found significant associations TAS-Total and the SC subscale of CBCL (p < 0.05), and factor 1 of TAS and WD of CBCL on boy alone (p < 0.05). However, negative related association was noted between TAS-F3 and the SP subscale of CBCL (p < 0.05).
Conclusion
We infer that when mothers are less able to distinguish internal emotional feelings, children's emotions and behaviors tend to be internalized, and they are more likely to show withdrawal, complaints of physical discomfort, and depression and anxiety. In addition, boys tend to show withdrawal traits in the internalization dimension; girls tend to show depression and anxiety in that respectively. This study preliminarily shows that there is a partial correlation between mother’s alexithymia and children's emotional and behavioral disorders, and it is worth further exploring the impact of caregivers' emotional traits on children's growth in the future.
F-18 FDG PET/CT vs. RAI-WBS and neck USG in early diagnosis of recurrent differentiated thyroid cancer
Authors: Bakshi Jaimanti , Panda Naresh Kumar , Goyal Atul Kumar , Saini Jyoti , Baral Sovit Jung , Mittal Bhagwant Rai , Lal Anupam
Published: May 2022

Background: Differentiated thyroid cancer (DTC) contributes up to 90% of total thyroid malignancies, and due to high recurrence chances, post-operative surveillance is performed by regular clinical follow-ups with routine neck ultrasonography (USG) and radioactive iodine whole-body scan (RAI-WBS). Objectives: Positron emission tomography and computed tomography (F-18 FDG PET/CT) has emerged as promising tools for the early detection of many malignancies and therefore in the present study aims to analyze the effectiveness of F-18 FDG PET/CT 1 Springer Nature 2021 L A T E X template Recurrent differentiated thyroid cancer vs. RAI-WBS and neck USG in early diagnosis of recurrence in differentiated thyroid cancer after thyroidectomy. Methods: For this, total 30 patients diagnosed with the DTC were recruited in the study and were followed for the assessment of recurrence of the disease. Either F-18 FDG PET/CT or RAI-WBS and neck USG was used as a recurrence assessment tool and data was compared for the sensitivity and specificity of the tool. Results: The recurrence rate was found to be 26.67% in the present study. F-18 FDG PET/CT exhibited a significant advantage over RAI-WBS and neck USG for early detection of recurrence of DTC. F-18 FDG PET/CT was found to exhibit good sensitivity and high specificity compare to the RAI-WBS and neck USG for early detection of recurrence of DTC. Conclusion: F-18 FDG PET/CT is recommended to use in those cases which have a high suspicion of DTC recurrence but is marked negative in other imaging techniques.
Intersecting substance use treatment and harm reduction services: Exploring the characteristics and service-needs of a community-based sample of people who use drugs
Authors: Krawczyk Noa , Allen Sean T. , Schneider Kristin E. , Solomon Keisha , Shah Hridika , Morris Miles , Harris Samantha J. , Sherman Susan G. , Saloner Brendan
Published: May 2022

Background: Substance use treatment and harm reduction services are essential components of comprehensive strategies for reducing the harms of drug use and overdose. However, these services have been historically siloed, and there is a need to better understand how programs that serve people who use drugs (PWUD) are integrating these services. In this study, we compared treatment and harm reduction services offered by a multistate sample of substance use service providers and assessed how well they align with characteristics and needs of clients they serve early in the COVID-19 pandemic. Methods: We recruited a convenience sample of programs that deliver harm reduction and/or treatment services in 10 states. Program directors participated in a survey assessing the services offered at their program. We also recruited clients of these programs to participate in a survey assessing a range of sociodemographic and health characteristics, substance use behaviors, and health service utilization. We then cross-compared client characteristics and behaviors relative to services being offered through these programs. Results: We collected and analyzed data from 511 clients attending 18 programs which either offered treatment with medications for opioid use disorder (MOUD) (N=6), syringe service programs (SSP) (N=8), or both MOUD and SSP (N=4). All programs delivered a range of treatment and harm reduction services, with MOUD+SSP programs delivering the greatest breadth of services. There were discrepancies between services provided and characteristics and behaviors reported by clients: 80% of clients of programs that offered MOUD without SSP actively used drugs and 50% injected drugs; 40% of clients of programs that offered SSP without MOUD sought drug treatment services. Approximately half of clients were unemployed and unstably housed, but few programs offered direct social services.Conclusions: In many ways, existing programs are not meeting the service needs of PWUD. Investing in innovative models that empower clients and integrate a range of accessible and flexible treatment, harm reduction and social services can pave the way for a more effective and equitable service system that considers the long-term health of PWUD.
Landscape drivers and spatial scale predict epiphyte biodiversity across human-modified landscapes
Authors: PARRA-SANCHEZ Edicson , EDWARDS David
Published: May 2022

ContextHuman-driven landscape processes such as habitat loss and fragmentation act on biodiversity but their effects are mediated by spatial scale. Quantification of the effects of the spatial scale of landscape processes on biodiversity moves beyond a purely ecological concern, towards a pragmatic tool for conservation aiming to designing biodiversity-rich landscapes.ObjectivesWe conducted a multiscale study to quantify the direction of effect (positive, negative, or neutral) and spatial scale (scale of effect, SoE) that three landscape processes—habitat loss (forest cover), fragmentation (number of patches), and edge effects (edge density)—exert independently on epiphyte biodiversity. MethodsWe focused on hyperdiverse vascular epiphytes and orchid communities, sampled across 23 human-modified landscapes in the mountainous region of the eastern Colombian Andes. We consider 12 spatial scales ranging from 100-2400 m radii and use metrics of Hill numbers to quantify biodiversity responses. ResultsHabitat loss and edge effects act at fine spatial scales (SoE= 200 m radius), predicting low species richness and abundance distributions across epiphytes and orchid communities. Fragmentation negatively impacts orchid communities at coarse scales (SoE= 2400 m radius). However, the biodiversity-landscape relationship might be undetected if assessed at the incorrect spatial scales (Forest cover 800-1500 m; Fragmentation ≥ 300 m; and edge density ≤800 radii).ConclusionsWe showed that habitat loss, fragmentation, and edge effects all play a negative role on biodiversity, and their detectability and impact is scale dependant. To design landscapes that are beneficial for mountainous plant biodiversity, it is important to improve forest cover as well as to reduce fragmentation and edge effects.
Rare sugar L-sorbose exerts antitumor activity by impairing glucose metabolism
Authors: Gao Xiao-Dong , Xu Hui-Lin , Zhou Xiaoman , Chen Shuai , Xu Si , Li Zijie , Nakanishi Hideki
Published: May 2022

Rare sugars are monosaccharides with low natural abundance. They are structural isomers of dietary sugars, but hardly be metabolized. Here, we report that rare sugar l-sorbose induces apoptosis in various cancer cells. As a C-3 epimer of d-fructose, l-sorbose is internalized via the transporter GLUT5 and phosphorylated by ketohexokinase (KHK) to produce l-sorbose-1-phosphate (S-1-P). Cellular S-1-P inactivates the glycolytic enzyme hexokinase resulting in attenuated glycolysis. Consequently, mitochondrial function is impaired and reactive oxygen species are produced. Moreover, l-sorbose downregulates the transcription of KHK-A, a splicing variant of KHK. Since KHK-A is a positive inducer of antioxidation genes, the antioxidant defense mechanism in cancer cells can be attenuated by l-sorbose-treatment. Thus, l-sorbose performs multiple anticancer activities to induce cell apoptosis. In mouse xenograft models, l-sorbose inhibits the proliferation of tumors either alone or in combination with other anticancer drugs. These results demonstrate l-sorbose as an attractive therapeutic reagent for cancer treatment.
The Clinical Characteristic and Management of patients with nocardiosis in a tertiary hospital in China
Authors: Liu Peilin , Wang Zhiqian , Jian Zijuan , Liu Xuan , Li Yanming , Yan Qun , Zhong Baiyun , Liang Xianghui , Liu Wenen
Published: May 2022

Background: Nocardiosis is an uncommon opportunistic bacterial infection caused by Nocardia spp., and its incidence has increased significantly in recent years. This study aimed to summarize the clinical characteristics and management of nocardiosis to provide a reference for clinical diagnosis and treatment.Methods : This retrospective study was conducted based on the medical records of nocardiosis patients between January 2015 and December 2021 in a tertiary hospital in China.Results: Overall, 44 patients with nocardiosis were included in this analysis including 26 males and 18 females with a mean age of 50.4 ± 13.2 years. Connective tissue disease (CTD) was the most common underlying disease (16/44). Twenty-six patients were given immunosuppressive therapy. The most frequent infection sites were the lungs (17/44) and skin and soft tissue (8/44). Common symptoms included cough (23/44), expectoration (18/44), fever (15/44), and subcutaneous abscesses (15/44). Average microbiology laboratory detection time was 4.8 days. Twenty-nine patients were managed only with antimicrobial treatment, and 14 patients received both antimicrobial and surgical therapies. Trimethoprim-sulfamethoxazole (TMP-SMX) was the most commonly used antibiotic (31/44). Thirty-five patients were cured or improved, 6 patients were discharged from the hospital due to poor prognosis, and 1 patient died.Conclusion : Clinicians should be alert to immunocompromised patients, especially those with CTD, and tend to pulmonary or cutaneous infections. Smear microscopic examination and prolonged culture time may aid Nocardia identification. Patients with nocardiosis can be treated with antimicrobials or surgical therapy. TMP-SMX remains the mainstay treatment, and its early and prompt use may improve nocardiosis outcome.
Damage Analysis and Prediction in Glass Fiber Reinforced Polyester Composite Using Acoustic Emission and Machine Learning
Authors: S.Gholizadeh
Published: May 2022

One of the most pervasive types of structural problems in aircraft industries is fatigue cracking that can potentially occur without anticipation with catastrophic failures and unexpected downtime. Acoustic emission is a passive structural health monitoring (SHM) technique, since it offers real time damage detection based on stress waves generated by cracking in the structure. Machine learning techniques have presented great success over the past few years with a large number of applications. This study assesses the progression of damage occurring on glass fiber reinforced polyester composite specimens using two approaches of machine learning, namely, Supervised and Unsupervised learning. A methodology for damage detection and characterization of composite is presented. The result shows that machine learning can predict the failure. All predictive models and their performance as well as AE parameters had a direct relationship with the applied stress values, suggesting that these correlation coefficients are reliable means of predicting fatigue life in a composite material.
A retrospective evaluation of short-term results from colonic stenting as a bridge to elective surgery versus emergency surgery for malignant intestinal obstruction
Authors: Chongjing Mu , Lei Chen
Published: May 2022

Purpose
The efficacy and safety of self-expanding metallic stent (SEMS) placement as a bridge to elective surgery versus emergency surgercy to treat malignant intestinal obstruction is debated. This study aimed to evaluate the outcomes of patients with malignant intestinal obstruction treated using different procedure.
Methods
Subjects admitted to the authors’ department with colonic obstruction (n = 87) were studied. They underwent colonic stenting as a bridge to elective surgery (SEMS group: n = 14) or emergency surgery (ES group:n = 22).Their demographic characteristics, stoma rate, laparoscopy rate and postoperative complications were analyzed, and the potential risk factors of postoperative complications and the optimal time interval from SEMS implantation to elective surgery were explored.
Results
The stoma rate was 15.4% in the SEMS group versus 60.0% in the ES group (P = 0.015), and the postoperative co mplication rate was 7.7% in the SEMS group versus 40.0% in the ES group (P = 0.042). The proportion of patients undergoing laparoscopy in SEMS group was significantly higher than that in ES group (69.2% vs. 15.0%; P = 0.003).The effect of ASA gradeification on postoperative complications was statistically significant (OR = 24.565; P = 0.008). The Receiving operating characteristic(ROC) curve could not determine the optimal time interval between SEMS implantation and elective surgery (AUC = 0.466).
Conclusions
SEMS implantation has the advantages of lower temporary stoma rate, less postoperative complications and higher laparoscopy rate compared with ES in the treatment of left malignant intestinal obstruction. ASA grade is a risk factor for postoperative complications. However, larger sample size prospective randomized controlled trials(RCT) are still needed to confirm long-term oncological outcomes.
Testing persistence of ammonia emissions with historical data from 1770 to 2019 in 37 OECD countries
Authors: Sakiru Solarin , Bermejo Lorenzo , Gil-Alana Luis A.
Published: May 2022

We examine the historical time series data of ammonia emissions from 1770 to 2019 in 37 OECD countries by looking at its statistical properties in order to determine if the series display time trends and persistence. These two properties are very common in environmental data, and our results indicate that reversion to the mean only occurs in the case of Finland, while the null hypothesis of a unit root cannot be rejected in the case of Norway or Iceland. In all the other cases, the estimated value of the differencing parameter is much higher than 1, and this is consistent for the two assumptions made regarding the error term. Thus, shocks are expected to be permanent in all cases except Finland.
Prognostic significance of CDC20 expression in malignancy patients: a meta-analysis
Authors: Xian Feng , Yang Xuegang , Xu Guohui
Published: May 2022

Background: A plenty of previous researches have reported the prognostic value of Cell Division Cycle Protein 20(CDC20) in malignancy tumors. Nevertheless, these researches were restricted by the small sample databases and the results were not strongly consistent among them.Methods: Databases involving EMBASE, PubMed, Cochrane Library and Web of Science were carefully searched, and literatures investigating the prognostic effect of aberrantly expressing CDC20 among human cancers were collected for further analysis. We used both hazards ratios and its corresponding 95% confidence intervals to evaluate the connection among the survival rate of malignancy patients and the expression of CDC20.Results: There were 10 eligible researches involving 2342 participants which were included in this study. The outcomes suggested that highly expressing CDC20 was significantly correlated with worse clinical prognosis of overall survival (OS) (HR 2.52, 95% CI 2.13-2.99) in the multivariate analysis, that (HR 2.05, 95% CI 1.50-2.82) in the univariate analysis and recurrence-free survival (HR 2.08, 95% CI 1.46-2.98). Moreover, we discovered that elevated CDC20 expression was significantly connected with decreased overall survival in non-small cell lung cancer (HR 2.40, 95% CI 1.91-3.02) while no significant relevance between highly expressing CDC20.Conclusions: this meta-analysis demonstrated that highly expressing CDC20 might be an unfavorable prognostic biomarker for various malignancy patients, which can make great contributions to the clinical diagnosis and treatment.
Soil erosion control from trash residues at varying land slopes under simulated rainfall conditions
Authors: Singh Sachin Kumar , Vishwakarma Dinesh Kumar , Kashyap P. S. , Kumar Akhilesh , Kumar Pankaj , Kumar Rohitashw
Published: May 2022

Trash mulches are very effective in preventing soil erosion; reduce sediment transport rate, runoff rate and increasing infiltration. The study was carried out with the objectives to observe the sediment outflow from sugar cane leaf (trash) mulch treatments at selected land slopes under simulated rainfall conditions by using rainfall simulator of size 10 m × 1.2 m × 0.5 m with the locally available soil material collected from Pantnagar. In the present study, trash mulches with different quantities were selected to observe the effect of mulching in soil loss reduction. The quantity of mulche were taken as, 6 t/ha, 8 t/ha and 10 t/ha, three rainfall intensities viz. 11cm/h, 13cm/h and 14.65cm/h at 0%, 2% and 4% land slopes were selected. The duration of rainfall was fixed (10 minutes) for every mulch treatment. The total runoff volume was found to be varying with different mulch rates for particular rainfall input and land slope. The runoff distribution pattern was observed to be increasing with the increase in land slope. The average sediment concentration (SC) and outflow was found to be increasing with the increasing land slope, but SC and outflow decreased with increasing mulch rate for particular land slope and rainfall intensity. The SOR (SOR) for no mulch treated land was higher as compared to trash mulch treated lands. Mathematical relationships were developed for relating SOR, SC, land slope and rainfall intensity for a particular mulch treatment. It was observed that values of SOR and average SC had a good correlation with rainfall intensity and land slope for each mulch treatment. The correlation coefficients of developed models were found to be more than 90%.
Reduced immunogenicity and endowed antimicrobial activity in β-lactoglobulin by preparing edible bioconjugate
Authors: Yoshida Tadashi , Ikeda Risa , Kawai Shinya , Horiuchi Kengo , Matsuo Yuho , Hattori Makoto
Published: May 2022

β-lactoglobulin (BLG) and ε-polylysine (PL) were bound by using microbial transglutaminase. Dextran (Dex) was further conjugated to the BLG-PL conjugate by the Maillard reaction. Confirmation of conjugation was carried out by SDS-PAGE. From the results of isoelectric focusing, it was revealed that the isoelectric point of the BLG-PL conjugate was shifted to the basic side as compared with native BLG. Immunogenicity of BLG in BALB/c mice was lowered by conjugation with PL and further lowered by conjugation with Dex. By conjugation with PL and PL-Dex, antibacterial activity against S. aureus was endowed to BLG. Because the conjugation method in this study is a safe method, it is valuable in that it can be applicable to food processing.
Transient suppression of ovarian function Induced by Dulaglutide: a case report and review of the literature
Authors: Guo Yangyang , Xie Mengchen , Liu Yuting , Huang Jinhui , Wang Qiong , Wang Xin
Published: May 2022

BackgroundGlucagon-like peptide-1 (GLP-1) agonist (GLP-1RA) is a new type of hypoglycemic agent with profound effects on the reproductive system. We report a case of ovarian function suppressed by the long-acting GLP-1RA dulaglutide.Case presentationA 41-year-old Chinese female presented with amenorrhea after treatment with GLP-1RA dulaglutide for type 2 diabetes. Her sex hormone measurement showed that the levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were increased, while estradiol(E2) level was decreased. Her symptoms improved after the stoppage of dulaglutide. However, LH and FSH levels were still above the normal range two months after discontinuing the injection of dulaglutide.ConclusionThe long-acting GLP-1RA dulaglutide may transiently suppress ovarian function. Because of their different structures and half-times, the individual impact on the reproductive system of each type of GLP-1RA should be monitored in the future.
Quantitative evaluation of pulmonary hypertension using 4D flow MRI: a retrospective study
Authors: Koike Hirofumi , Sueyoshi Eijun , Nishimura Takamasa , Morikawa Minoru , Uetani Masataka
Published: May 2022

PurposePulmonary hypertension (PH) is a severe vascular disorder that may affect 50% of patients with heart failure. Currently, right-sided heart catheterization is required to definitively diagnose PH. However, this method is invasive and thus may not be appropriate for repeated, long-term monitoring of PH patients. This retrospective study’s aim was to evaluate whether 4D flow magnetic resonance imaging (MRI) can be used to quantitively measure flow parameters to identify patients with PH. MethodsThe study cohort included 97 patients recruited from a single institution and divided into three groups based on echocardiographic estimate of pulmonary artery systolic pressure (PASP): normal group with PASP<36mmHg, borderline PH group with PASP of 37–50mmHg, and PH group with PASP>50mmHg. 4D flow MRI was used to quantitively assess blood flow and velocity, regurgitation, wall shear stress (WSS) and kinetic energy in the pulmonary artery trunk, right main pulmonary artery, and left pulmonary artery. Two experienced radiologists independently analyzed the MR images, blinded to clinical details. ResultsWe found a significant difference in WSS in the pulmonary artery trunk, right main pulmonary artery and left main pulmonary artery among the three patient groups. We also found significant differences in the kinetic energy and average through velocity in the pulmonary artery trunk and right main pulmonary artery, and significant differences in the flow rate in the right main pulmonary artery. ConclusionsThese data suggest that 4D flow MRI can quantitate pulmonary artery flow parameters and distinguish between patients with and without PH.
Automatic Comprehensive Aspects Reports in Clinical Acute Stroke MRIs
Authors: Faria Andreia , Liu Chin-Fu , Li Alice , Kim Ganghyun , Miller Michael , Hillis Argye
Published: May 2022

The Alberta Stroke Program Early CT Score (ASPECTS) is a simple visual system to assess the extent and location of ischemic stroke core. The capability of ASPECTS for selecting patients’ treatment, however, is affected by the variability in human evaluation. In this study, we developed a fully automatic system to calculate ASPECTS comparable with consensus expert readings. Our system was trained in 400 clinical diffusion weighted images (DWIs) of patients with acute infarcts and evaluated with an external testing set of 100 cases. The models are interpretable, and the results are comprehensive, evidencing the features that lead to the classification. This system adds to our automated pipeline for acute stroke detection, segmentation, and quantification in MRIs (ADS), which outputs digital infarct masks and the proportion of diverse brain regions injured, in addition to the predicted ASPECTS, the prediction probability and the explanatory features. ADS is public, free, accessible to non-experts, has very few computational requirements, and run in real time in local CPUs with a single command line, fulfilling the conditions to perform large-scale, reproducible clinical and translational research
THE CONCEPT OF STRESS RESILIENCE AS METHODOLOGICAL APPROACH IN STUDYING ACCOUNTS OF KUZBASS DEPUTIES ON THE VKONTAKTE SOCIAL NETWORK
Authors:   Kemerovo State University, Kemerovo, Matveeva E. , Alagoz A. ,   Kemerovo State University, Kemerovo
Published: May 2022

The subject of the article is related to the identification of possible risk factors in the communication of Kuzbass members of the State Duma of the VIII convocation based on the case of the VKontakte social network, which affect the stress resilience of the regional political system in Kuzbass. The purpose of the article is the validation of the authors’ approach to the application of the stress resilience concept to analyzing possible risk factors in the communication of State Duma members of the VIII convocation on the VKontakte network and the identification of stabilizing factors that ensure the stability of all branches of the regional power system. The methodological basis of the study is presented by the concept of resilience, the M. Castells’s concept of the network society and the method of content analysis for studying posts and comments on the posts of deputies. The concept of stress resilience was first substantiated in the works of K. Holling in his consideration of resilience of socio-ecological systems. Russian researchers do not apply the concept of resilience widely; it is applied mainly to the consideration of international issues related to the dynamics of interaction between Russia and the states of the European Union. The first works on the impact of risk factors on the internal political processes of the regions have appeared recently, in particular, against the background of the spread of COVID-19 and under the influence of latent risks of the socio-economic development of the regions. In the course of the study, it was revealed that the accounts of Kuzbass deputies have all the signs of risk factors that not only make the posted news content unattractive, but also affect the sentiments in the region and increase the level of political inertia and apathy towards all programs and projects implemented in the region. News content is formalized, posts have features of low information content, the opinion of politicians in posts is impersonal, and comments do not fulfill its function of receiving an answer to the question posed. Forms of communication are identified as stabilizing factors that contribute to building a more “open” dialogue and exchange of opinions between politicians and network users as a tool for ensuring the sustainable development of Kuzbass
Methodology for efficiency evaluation of real investments with exceptional cash flow configuration
Authors:   St. Petersburg State Marine Technical University, Petersburg, Kolobkova I.
Published: May 2022

The methodology for efficiency evaluation of investment projects, concerning the calculation of several key indicators and making a decision on the advisability of project approval, is well-known in scientific spheres. It is based on the algorithm for evaluating portfolio investments, when the investment itself is initially and simultaneous. Further it generates a certain revenue flow to the owner of a security or physical asset. Real investments, such as capital or fixed assets investments are much more complex and diversified in their structure. The cash flow can naturally start with an influx of funds at the very beginning and then finish with the outflow. Frequently, the capital investment itself is not a single way of infusion, that's why the use of standard approaches often leads to a formal absence or, on the contrary, multitude of the internal rent rates and also causes uncertainty and problems with calculations of the payback period for purely mathematical reasons. Net Present Value can be defined by default in any situation, but the Internal Rate of Return specifically serves as the main relative and comparable indicator for both the project developer and potential investors in present conditions. This article offers the alternative algorithm that allows you to review the full-scale financial and economic characteristics of the project, regardless of the cash flow pattern under any circumstances. The divergences between the known and the proposed methodology are minimal, as the author explains us by researching the examples of the "ordinary" projects. Thus, this model can be considered as reasonable for evaluation purposes in comparison with basic methods, which are not intended to provide such an analysis in general. The object of the proposed research is real investment projects with signs of extraordinarity, the goal is to develop methodological approaches for their correct financial and economic evaluation. The methodological base is the apparatus of financial mathematics, the theory of efficiency and the theory of managerial decision-making. The key result is two approaches that always allow to calculate internal rate of return and the exact payback period, regardless of the configuration of the cash flow. The article will be useful primarily to initiators of projects in existing and created production, as well as potential investors in such innovations.
Proteomic Analysis of Pollen–Stigma Interaction between Vitis rotundifolia and Vitis vinifera
Authors: Wang Bo , Wu Weimin , Wang Xicheng , Wang Zhuangwei , Qian Yaming
Published: May 2022

Muscadine grape (Vitis rotundifolia) is highly resistant to many diseases and insects that attack european grape (Vitis vinifera). However, distant hybridization incompatibility between V. rotundifolia (female) and V. vinifera (male) impedes the utilization of V. rotundifolia in grape breeding. This study used fourth-dimension label-free protein quantitation to detect the key genes and pathways in the V. rotundifolia stigma after self-pollination (V. rotundifolia × V. rotundifolia) and cross-pollination (V. rotundifolia × V. vinifera). A histological analysis showed that pollen tube growth in the stigma of V. rotundifolia was arrested 8 hours after cross-pollination, but not after self-pollination. A proteomic analysis identified 32 differentially expressed proteins (DEPs) in the stigma of V. rotundifolia between self-pollination and cross-pollination. A heatmap analysis grouped these DEPs into four clusters. The top gene ontology terms were ATPase-coupled transmembrane transporter activity, extracellular region, DNA replication, and cellular carbohydrate biosynthetic process. A Kyoto Encyclopedia of Genes and Genomes analysis revealed that these DEPs participated in DNA replication and starch and sucrose metabolism pathways. The downregulated A5AY88, D7TJ35, D7SU26, F6HJI1, and F6GUE7 may have a role in cross incompatibility. This study revealed the cross incompatibility of grapes at histological and proteomic levels.
Self-Management Knowledge among Patients with Type 2 Diabetes Mellitus in Hospital Taiping, Malaysia
Authors:   Faculty of Pharmacy, Universiti Teknologi MARA, Cawangan Pulau Pinang, Kampus Bertam, Pulau Pinang, Malaysia, Mohamad Fadzil Maisarah , Mohd Akmal Muhammad Hadif Syahmi ,   Faculty of Pharmacy, Universiti Teknologi MARA, Cawangan Pulau Pinang, Kampus Bertam, Pulau Pinang, Malaysia, Wong Yuet Yen ,   Faculty of Pharmacy, Universiti Teknologi MARA, Cawangan Pulau Pinang, Kampus Bertam, Pulau Pinang, Malaysia,   Collaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia, Neoh Chin Fen ,   Faculty of Pharmacy, Universiti Teknologi MARA, Cawangan Selangor, Kampus Puncak Alam, Selangor, Malaysia,   Collaborative Drug Discovery Research (CDDR) Group, Pharmaceutical and Life Sciences Community of Research, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia, Ying Lean Qi ,   Faculty of Pharmacy, Universiti Teknologi MARA, Cawangan Pulau Pinang, Kampus Bertam, Pulau Pinang, Malaysia,   Vector-Borne Diseases Research Group (VERDI), Pharmaceutical and Life Sciences CoRe, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia
Published: May 2022

Type 2 diabetes mellitus (T2DM) is characterised by hyperglycaemia resulting from insulin insufficiency, insulin resistance or both. Although different anti-diabetic agents are available to control blood glucose, patient self-management is essential for achieving good glycaemic control. Good knowledge of disease self-management is a pre-requisite to enable patients in making informed decisions in disease management. In this study, we aimed to determine the knowledge of self-management among patients with T2DM visiting outpatient pharmacy at Hospital Taiping. Adult patients with T2DM were invited to participate in a survey from December 2018 to February 2019. A pre-validated, selfadministered questionnaire was used to capture patients’ demographics, glucose level and diabetes self-management knowledge. A total of 148 patients responded to the survey. More than half of the patients (54.7%) in this study were found to have low knowledge scores (< 70%). The level of education (p = 0.041), occupation (p = 0.024) and the use of insulin (p < 0.001) were significantly associated with patients’ level of self-management knowledge whereas no significant relationships were found between knowledge score across age, gender, ethnicity, living status, use of oral anti-diabetic agents, attending diabetes education before and the duration of diabetes. A low but significant negative correlation was found between the score of self-management knowledge and fasting blood glucose (r = −0.264, p = 0.002). Despite the fact that nearly half of the respondents had good knowledge scores, the knowledge gaps remain to be filled s o t hat p atients are empowered to practise self-management in managing their T2DM.
Inclusive decays of heavy quark hybrids
Authors: Mohapatra Abhishek
Published: May 2022

In order to understand the nature of the XYZ particles, theoretical predictions of the various XYZ decay modes are essential. In this work, we focus on the semi-inclusive decays of heavy quarkonium hybrids into traditional quarkonium in the EFT framework. We begin with weakly coupled potential NRQCD effective theory that describes systems with two heavy quarks and incorporates multipole expansions and use it to develop a Born-Oppenheimer effective theory (BOEFT) to describe the hybrids and compute the semi-inclusive decay rates. We compute both the spin-conserving and spin-flipping decay rates and find that our numerical results of the decay rates are different from the previous studies. We also develop a systematic framework in which the theoretical uncertainty can be systematically improved.
Chiral symmetry restoration with three chiral partners
Authors: Torres-Rincon Juan
Published: May 2022

I discuss the masses of chiral partners in the context of chiral symmetry restoration at finite temperature. Using the Nambu--Jona-Lasinio model I first remind the usual situation where two mesons of opposed parity become degenerate above the chiral transition temperature. Then I consider an effective theory for D mesons where the positive parity companion presents a ``double pole structure''. In this case three different masses need to be analyzed as functions of the temperature. I suggest a possible restoration pattern at high temperatures when the back-reaction of the quark condensate is incorporated.
MANAGEMENT OF TONGUE-TIE USING 980 NM DIODE LASER - CASE SERIES
Authors: K. Arun , T.S Reshmi , Golla Sujitha , Jazil Ahmed Mohamed , Hari Amal , Varughese Ancy
Published: May 2022

The lingual frenulum is a vertical fold of mucous membrane that connects the tongue to the mouth floor. This congenital defect can result in diastema, difficulty moving the tongue, feeding problems, speech problems, and other mechanical and social challenges. A persons social communication and mechanical issues are exacerbated if this abnormality is not diagnosed early. Speech issues can be resolved by speech therapy if doctors discover and repair this aberration in early childhood via frenectomy, however owing to the ignorance of the individuals parents and clinicians, this anomaly occasionally goes unnoticed and causes speech problems throughout life. As a result, ankyloglossia treatment should be addressed at any age based on a risk-benefit analysis. The current study looks at two case reports of ankyloglossia, or tongue-tie, that were successfully treated using a diode laser.
PROSTHETIC REHABILITATION OF ENDODONTICALLY TREATED TEETH USING ENDOCROWN : A MODERN CONSERVATIVE APPROACH - TWO CASE REPORTS
Authors:   Department Of Conservative Dentistry And Endodontics, AJ Institute Of Dental Sciences, Rajiv Gandhi University Of Health Sciences, Karnataka, India., M.K Harikrishnan , P.A Jayalakshmi ,   Department Of Conservative Dentistry And Endodontics, AJ Institute Of Dental Sciences, Rajiv Gandhi University Of Health Sciences, Karnataka, India., Babu Jijo ,   Department Of Conservative Dentistry And Endodontics, AJ Institute Of Dental Sciences, Rajiv Gandhi University Of Health Sciences, Karnataka, India., Damda Aftab ,   Department Of Conservative Dentistry And Endodontics, AJ Institute Of Dental Sciences, Rajiv Gandhi University Of Health Sciences, Karnataka, India.
Published: May 2022

Endocrowns are a potential alternative for endodontically treated posterior teeth that have been severely damaged. Endocrowns are indirect monoblock restorations that are held in place by the pulp chamber of endodontically treated teeth (ETT). The major goal is to establish a bonded biomimetic repair with minimal root canal invasiveness. When compared to traditional crowns with post and core, the clinical technique including endocrown creation may be deemed less difficult, more practical, and easier to accomplish. With a 6-month follow-up period, this article covers two case reports of endodontically treated teeth successfully managed by PFM based Endocrown.