bluuebunny/crossref_metadata_2025 huggingface dataset:
Bone marrow remodeling supports hematopoiesis in response to immune thrombocytopenia progression in mice
Authors: Herd Oliver J. , Rani Gulab Fatima , Hewitson James P. , Hogg Karen , Stone Andrew P. , Cooper Nichola , Kent David G. , Genever Paul G. , Hitchcock Ian S.
Published: August 2021

Immune thrombocytopenia (ITP) is an acquired autoimmune condition characterized by both reduced platelet production and the destruction of functionally normal platelets by sustained attack from the immune system. However, the effect of prolonged ITP on the more immature hematopoietic progenitors remains an open area of investigation. By using a murine in vivo model of extended ITP, we revealed that ITP progression drives considerable progenitor expansion and bone marrow (BM) remodeling. Single-cell assays using Lin–Sca1+c-Kit+CD48–CD150+ long-term hematopoietic stem cells (LT-HSCs) revealed elevated LT-HSC activation and proliferation in vitro. However, the increased activation did not come at the expense of LT-HSC functionality as measured by in vivo serial transplantations. ITP progression was associated with considerable BM vasodilation and angiogenesis, as well as a twofold increase in the local production of CXCL12, a cytokine essential for LT-HSC function and BM homing expressed at high levels by LepR+ BM stromal cells. This was associated with a 1.5-fold increase in LepR+ BM stromal cells and a 5.5-fold improvement in progenitor homing to the BM. The increase in stromal cells was transient and reverted back to baseline after platelet count returned to normal, but the vasculature changes in the BM persisted. Together, our data demonstrate that LT-HSCs expand in response to ITP and that LT-HSC functionality during sustained hematopoietic stress is maintained through an adapting BM microenvironment.
PRPI-SC: an ensemble deep learning model for predicting plant lncRNA-protein interactions
Authors: Zhou Haoran , Wekesa Jael Sanyanda , Luan Yushi , Meng Jun
Published: August 2021

Background

Plant long non-coding RNAs (lncRNAs) play vital roles in many biological processes mainly through interactions with RNA-binding protein (RBP). To understand the function of lncRNAs, a fundamental method is to identify which types of proteins interact with the lncRNAs. However, the models or rules of interactions are a major challenge when calculating and estimating the types of RBP.

Results

In this study, we propose an ensemble deep learning model to predict plant lncRNA-protein interactions using stacked denoising autoencoder and convolutional neural network based on sequence and structural information, named PRPI-SC. PRPI-SC predicts interactions between lncRNAs and proteins based on the k-mer features of RNAs and proteins. Experiments proved good results on Arabidopsis thaliana and Zea mays datasets (ATH948 and ZEA22133). The accuracy rates of ATH948 and ZEA22133 datasets were 88.9% and 82.6%, respectively. PRPI-SC also performed well on some public RNA protein interaction datasets.

Conclusions

PRPI-SC accurately predicts the interaction between plant lncRNA and protein, which plays a guiding role in studying the function and expression of plant lncRNA. At the same time, PRPI-SC has a strong generalization ability and good prediction effect for non-plant data.
Cell-free fetal DNA testing and its correlation with prenatal indications
Authors: Wang Jing-wei , Lyu Yong-nan , Qiao Bin , Li Yan , Zhang Yan , Dhanyamraju Pavan Kumar , Bamme Yevgeniya , Yu Michael D. , Yang Dongqin , Tong Yong-qing
Published: August 2021

Background

The prenatal test of cell-free fetal DNA (cffDNA) is also known as noninvasive prenatal testing (NIPT) with high sensitivity and specificity. This study is to evaluate the performance of NIPT and its clinical relevance with various clinical indications.

Methods

A retrospective analysis was conducted on 14,316 pregnant women with prenatal indications, including advanced maternal age (≥35 years), maternal serum screening abnormalities, the thickened nuchal translucency (≥2.5 mm) and other ultrasound abnormalities, twin pregnancy/IVF-ET pregnancy, etc. The whole-genome sequencing (WGS) of maternal plasma cffDNA was employed in this study.

Results

A total of 189 (1.32%) positive NIPT cases were identified, and 113/189 (59.79%)cases were confirmed by invasive prenatal testing. Abnormal serological screening (53.14%) was the most common indication, followed by elderly pregnancy (23.02%). The positive prediction value for T21, T18, T13, sex chromosome abnormalities, other autosomal aneuploidy abnormalities, and CNV abnormalities were 91.84, 68.75,37.50, 66.67, 14.29, and 6.45%, respectively. The positive rate and the true positive rate of nuchal translucency (NT) thickening were the highest (4.17 and 3.33%), followed by the voluntary requirement group (3.49 and 1.90%) in the various prenatal screening indications. The cffDNA concentration was linearly correlated with gestational age (≥10 weeks) and the positive NIPT group’s Z-score values.

Conclusions

whole-genome sequencing of cffDNA has extremely high sensitivity and specificity for T21, high sensitivity for T18, sex chromosome abnormalities, and T13. It also provides evidence for other abnormal chromosomal karyotypes (CNV and non-21/18/13 autosomal aneuploidy abnormalities). The cffDNA concentration is closely related to the gestational age and determines the specificity of NIPT. Our results highlight NIPT’s clinical significance, which is an effective prenatal screening tool for high-quality care of pregnancy.
Bone mineral density and its influencing factors in Chinese children with spinal muscular atrophy types 2 and 3
Authors: Peng Xiaoyin , Qu Yujin , Li Xiaohui , Liu Junting , Shan Xinying , Wang Jia , Song Fang
Published: August 2021

Background

Patients with spinal muscular atrophy (SMA) are at risk of decreased bone mineral density (BMD). The bone health status of Chinese patients with SMA has been poorly studied. We aimed to evaluate the BMD of children with SMA types 2 and 3 in mainland China and investigate its influencing factors.

Methods

Forty patients with a mean age of 5.5 years affected by SMA types 2 and 3 (n = 22 and n = 18, respectively) were enrolled between September 2017 and May 2019. Total body less head (TBLH) BMD, lumbar spine (LS) BMD, and body composition were measured using dual-energy X-ray absorptiometry (DXA). Serum bone metabolism markers and complete spinal radiographs were assessed. We utilized a linear regression model to explore the correlations between BMD and its related factors.

Results

A total of 67.5% (27/40) of patients were diagnosed with low BMD and 2.5% (1/40) were diagnosed with osteoporosis. The TBLH BMD and LS BMD Z-scores in children with SMA type 2 were significantly lower than those with SMA type 3. Both TBLH and LS BMD Z-scores tended to increase with the change of SMA subtypes from 2a-3b. Vitamin D insufficiency and deficiency were found in 37.5% (15/40) of the patients. Serum Ca, phosphorus (P), alkaline phosphatase (ALP) and parathormone (PTH) levels were normal. There were no significant differences among the four subtypes in terms of all the serum bone metabolism markers. Phenotype was significantly associated with TBLH BMD and LS BMD Z-scores, and serum PTH levels were significantly associated with TBLH BMD Z-scores.

Conclusions

Low BMD and osteoporosis were highly prevalent in mainland Chinese children with SMA types 2 and 3. Phenotype and serum PTH level might be the influencing factors of BMD. Regular monitoring of BMD by DXA scan and taking active interventions aim to SMA children with different types are important.
Effectiveness of a ‘do not interrupt’ vest intervention to reduce medication errors during medication administration: a multicenter cluster randomized controlled trial
Authors: Berdot Sarah , Vilfaillot Aurélie , Bezie Yvonnick , Perrin Germain , Berge Marion , Corny Jennifer , Thi Thuy Tan Phan , Depoisson Mathieu , Guihaire Claudine , Valin Nathalie , Decelle Claudine , Karras Alexandre , Durieux Pierre , Lê Laetitia Minh Maï , Sabatier Brigitte
Published: August 2021

Background

The use of a ‘do not interrupt’ vest during medication administration rounds is recommended but there have been no controlled randomized studies to evaluate its impact on reducing administration errors. We aimed to evaluate the impact of wearing such a vest on reducing such errors. The secondary objectives were to evaluate the types and potential clinical impact of errors, the association between errors and several risk factors (such as interruptions), and nurses’ experiences.

Methods

This was a multicenter, cluster, controlled, randomized study (March–July 2017) in 29 adult units (4 hospitals). Data were collected by direct observation by trained observers. All nurses from selected units were informed. A ‘Do not interrupt’ vest was implemented in all units of the experimental group. A poster was placed at the entrance of these units to inform patients and relatives. The main outcome was the administration error rate (number of Opportunities for Error (OE), calculated as one or more errors divided by the Total Opportunities for Error (TOE) and multiplied by 100).

Results

We enrolled 178 nurses and 1346 patients during 383 medication rounds in 14 units in the experimental group and 15 units in the control group. During the intervention period, the administration error rates were 7.09% (188 OE with at least one error/2653 TOE) for the experimental group and 6.23% (210 OE with at least one error/3373 TOE) for the control group (p = 0.192). Identified risk factors (patient age, nurses’ experience, nurses’ workload, unit exposition, and interruption) were not associated with the error rate. The main error type observed for both groups was wrong dosage-form. Most errors had no clinical impact for the patient and the interruption rates were 15.04% for the experimental group and 20.75% for the control group.

Conclusions

The intervention vest had no impact on medication administration error or interruption rates. Further studies need to be performed taking into consideration the limitations of our study and other risk factors associated with other interventions, such as nurse’s training and/or a barcode system.

Trial registration

The PERMIS study protocol (V2–1, 11/04/2017) was approved by institutional review boards and ethics committees (CPP Ile de France number 2016-A00211–50, CNIL 21/03/2017, CCTIRS 11/04/2016). It is registered at ClinicalTrials.gov (registration number: NCT03062852, date of first registration: 23/02/2017).
A qualitative assessment of barriers and facilitators associated with addressing social determinants of health among members of a health collaborative in the rural Midwest
Authors: Dauner Kim Nichols , Loomer Lacey
Published: August 2021

Purpose

Rural communities have unique economic and social structures, different disease burdens, and a more patchworked healthcare delivery system compared to urban counterparts. Yet research into addressing social determinants of health has focused on larger, urban, integrated health systems. Our study sought to understand capacities, facilitators, and barriers related to addressing social health needs across a collaborative of independent provider organizations in rural Northeastern Minnesota and Northwestern Wisconsin.

Methods

We conducted qualitative, semi-structured interviews with a purposive sample of 37 key informants from collaborative members including 4 stand-alone critical access hospitals, 3 critical access hospitals affiliated with primary care, 1 multi-clinic system, and 1 integrated regional health system.

Findings

Barriers were abundant and occurred at the organizational, community and policy levels. Rural providers described a lack of financial, labor, Internet, and community-based social services resources, a limited capacity to partner with other organizations, and workflows that were less than optimal for addressing SDOH. State Medicaid and other payer policies posed challenges that made it more difficult to use available resources, as did misaligned incentives between partners. While specific payer programs and organizational innovations helped facilitate their work, nothing was systemic. Relationships within the collaborative that allowed sharing of innovations and information were helpful, as was the role leadership played in promoting value-based care.

Conclusions

Policy change is needed to support rural providers in this work. Collaboration among rural health systems should be fostered to develop common protocols, promote value-based care, and offer economies of scale to leverage value-based payment. States can help align incentives and performance metrics across rural health care entities, engage payers in promoting value-based care, and bolster social service capacity.
Emerging epidemics: is the Zanzibar healthcare system ready to detect and respond to mosquito-borne viral diseases?
Authors: Saleh Fatma , Kitau Jovin , Konradsen Flemming , Mboera Leonard E.G. , Schiøler Karin L.
Published: August 2021

Background

Effective control of emerging mosquito-borne viral diseases such as dengue, chikungunya, and Zika requires, amongst other things, a functional healthcare system, ready and capable of timely detection and prompt response to incipient epidemics. We assessed the readiness of Zanzibar health facilities and districts for early detection and management of mosquito-borne viral disease outbreaks.

Methods

A cross-sectional study involving all 10 District Health Management Teams and 45 randomly selected public and private health facilities in Zanzibar was conducted using a mixed-methods approach including observations, document review, and structured interviews with health facility in-charges and District Health Management Team members.

Results

The readiness of the Zanzibar healthcare system for timely detection, management, and control of dengue and other mosquito-borne viral disease outbreaks was critically low. The majority of health facilities and districts lacked the necessary requirements including standard guidelines, trained staff, real-time data capture, analysis and reporting systems, as well as laboratory diagnostic capacity. In addition, health education programmes for creating public awareness and Aedes mosquito surveillance and control activities were non-existent.

Conclusions

The Zanzibar healthcare system has limited readiness for management, and control of mosquito-borne viral diseases. In light of impending epidemics, the critical shortage of skilled human resource, lack of guidelines, lack of effective disease and vector surveillance and control measures as well as lack of laboratory capacity at all levels of health facilities require urgent attention across the Zanzibar archipelago.
Quantifying the short-term effects of air pollution on health in the presence of exposure measurement error: a simulation study of multi-pollutant model results
Authors: Evangelopoulos Dimitris , Katsouyanni Klea , Schwartz Joel , Walton Heather
Published: August 2021

Background

Most epidemiological studies estimate associations without considering exposure measurement error. While some studies have estimated the impact of error in single-exposure models we aimed to quantify the effect of measurement error in multi-exposure models, specifically in time-series analysis of PM2.5, NO2, and mortality using simulations, under various plausible scenarios for exposure errors. Measurement error in multi-exposure models can lead to effect transfer where the effect estimate is overestimated for the pollutant estimated with more error to the one estimated with less error. This complicates interpretation of the independent effects of different pollutants and thus the relative importance of reducing their concentrations in air pollution policy.

Methods

Measurement error was defined as the difference between ambient concentrations and personal exposure from outdoor sources. Simulation inputs for error magnitude and variability were informed by the literature. Error-free exposures with their consequent health outcome and error-prone exposures of various error types (classical/Berkson) were generated. Bias was quantified as the relative difference in effect estimates of the error-free and error-prone exposures.

Results

Mortality effect estimates were generally underestimated with greater bias observed when low ratios of the true exposure variance over the error variance were assumed (27.4% underestimation for NO2). Higher ratios resulted in smaller, but still substantial bias (up to 19% for both pollutants). Effect transfer was observed indicating that less precise measurements for one pollutant (NO2) yield more bias, while the co-pollutant (PM2.5) associations were found closer to the true. Interestingly, the sum of single-pollutant model effect estimates was found closer to the summed true associations than those from multi-pollutant models, due to cancelling out of confounding and measurement error bias.

Conclusions

Our simulation study indicated an underestimation of true independent health effects of multiple exposures due to measurement error. Using error parameter information in future epidemiological studies should provide more accurate concentration-response functions.
Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells
Authors: Li Lijie , Liao Zhouzhou , Ye Mingzhu , Jiang Jianfa
Published: August 2021

Background

Endometriosis is a serious reproductive and general health consequences. Recombinant human IL-37 (rhIL-37) is an inhibitor of inflammation.

Methods

ELISA assay was performed to detect the concentration of cytokines. Flow cytometry was used to analyze cell proportion. Besides, qRT-PCR and western blotting assay were used to detect the level of gene and protein, respectively. Transwell co-culture system was used for the co-culture of dendritic cells (DCs) and CD4+T cells.

Results

Our data showed that rhIL-37 inhibited the development of ectopic lesions in the mice with endometriosis, increased Th1/Th2 ratio and induced DCs maturation. The co-culture system of DCs and CD4+T cells demonstrated that rhIL-37 increased Th1/Th2 cell ratio through promoting DCs maturation. Moreover, the expression of IL-4 in the DCs derived from healthy mice was inhibited by rhIL-37 treatment. rhIL-37 increased Th1/Th2 cell ratio through inhibiting IL-4 in DCs. Subsequently, our results proved that rhIL-37 promoted the maturation of DCs via inhibiting phosphorylation of STAT3. Activation of STAT3 could reverse rhIL-37-induced maturation of DCs.

Conclusion

Overall, rhIL-37 could protect against endometriosis through increasing the ratio of Th1/Th2 cells via inducing DCs maturation and inhibiting IL-4 expression in the DCs. Furthermore, rhIL-37 induced DCs maturation by inhibiting STAT3 phosphorylation. Our data confirmed the protective effect of rhIL-37 in endometriosis. These data may provide a novel idea for the treatment of the disease.

Graphical abstract
Co-receptor signaling in the pathogenesis of neuroHIV
Authors: Nickoloff-Bybel E. A. , Festa L. , Meucci O. , Gaskill P. J.
Published: August 2021

The HIV co-receptors, CCR5 and CXCR4, are necessary for HIV entry into target cells, interacting with the HIV envelope protein, gp120, to initiate several signaling cascades thought to be important to the entry process. Co-receptor signaling may also promote the development of neuroHIV by contributing to both persistent neuroinflammation and indirect neurotoxicity. But despite the critical importance of CXCR4 and CCR5 signaling to HIV pathogenesis, there is only one therapeutic (the CCR5 inhibitor Maraviroc) that targets these receptors. Moreover, our understanding of co-receptor signaling in the specific context of neuroHIV is relatively poor. Research into co-receptor signaling has largely stalled in the past decade, possibly owing to the complexity of the signaling cascades and functions mediated by these receptors. Examining the many signaling pathways triggered by co-receptor activation has been challenging due to the lack of specific molecular tools targeting many of the proteins involved in these pathways and the wide array of model systems used across these experiments. Studies examining the impact of co-receptor signaling on HIV neuropathogenesis often show activation of multiple overlapping pathways by similar stimuli, leading to contradictory data on the effects of co-receptor activation. To address this, we will broadly review HIV infection and neuropathogenesis, examine different co-receptor mediated signaling pathways and functions, then discuss the HIV mediated signaling and the differences between activation induced by HIV and cognate ligands. We will assess the specific effects of co-receptor activation on neuropathogenesis, focusing on neuroinflammation. We will also explore how the use of substances of abuse, which are highly prevalent in people living with HIV, can exacerbate the neuropathogenic effects of co-receptor signaling. Finally, we will discuss the current state of therapeutics targeting co-receptors, highlighting challenges the field has faced and areas in which research into co-receptor signaling would yield the most therapeutic benefit in the context of HIV infection. This discussion will provide a comprehensive overview of what is known and what remains to be explored in regard to co-receptor signaling and HIV infection, and will emphasize the potential value of HIV co-receptors as a target for future therapeutic development.
Pyroptosis at the forefront of anticancer immunity
Authors: Loveless Reid , Bloomquist Ryan , Teng Yong
Published: August 2021

Tumor resistance to apoptosis and the immunosuppressive tumor microenvironment are two major contributors to poor therapeutic responses during cancer intervention. Pyroptosis, a lytic and inflammatory programmed cell death pathway distinct from apoptosis, has subsequently sparked notable interest among cancer researchers for its potential to be clinically harnessed and to address these problems. Recent evidence indicates that pyroptosis induction in tumor cells leads to a robust inflammatory response and marked tumor regression. Underlying its antitumor effect, pyroptosis is mediated by pore-forming gasdermin proteins that facilitate immune cell activation and infiltration through their release of pro-inflammatory cytokines and immunogenic material following cell rupture. Considering its inflammatory nature, however, aberrant pyroptosis may also be implicated in the formation of a tumor supportive microenvironment, as evidenced by the upregulation of gasdermin proteins in certain cancers. In this review, the molecular pathways leading to pyroptosis are introduced, followed by an overview of the seemingly entangled links between pyroptosis and cancer. We describe what is known regarding the impact of pyroptosis on anticancer immunity and give insight into the potential of harnessing pyroptosis as a tool and applying it to novel or existing anticancer strategies.
SCD1, autophagy and cancer: implications for therapy
Authors: Ascenzi Francesca , De Vitis Claudia , Maugeri-Saccà Marcello , Napoli Christian , Ciliberto Gennaro , Mancini Rita
Published: August 2021

Background

Autophagy is an intracellular degradation system that removes unnecessary or dysfunctional components and recycles them for other cellular functions. Over the years, a mutual regulation between lipid metabolism and autophagy has been uncovered.

Methods

This is a narrative review discussing the connection between SCD1 and the autophagic process, along with the modality through which this crosstalk can be exploited for therapeutic purposes.

Results

Fatty acids, depending on the species, can have either activating or inhibitory roles on autophagy. In turn, autophagy regulates the mobilization of fat from cellular deposits, such as lipid droplets, and removes unnecessary lipids to prevent cellular lipotoxicity. This review describes the regulation of autophagy by lipid metabolism in cancer cells, focusing on the role of stearoyl-CoA desaturase 1 (SCD1), the key enzyme involved in the synthesis of monounsaturated fatty acids. SCD1 plays an important role in cancer, promoting cell proliferation and metastasis. The role of autophagy in cancer is more complex since it can act either by protecting against the onset of cancer or by promoting tumor growth. Mounting evidence indicates that autophagy and lipid metabolism are tightly interconnected.

Conclusion

Here, we discuss controversial findings of SCD1 as an autophagy inducer or inhibitor in cancer, highlighting how these activities may result in cancer promotion or inhibition depending upon the degree of cancer heterogeneity and plasticity.
Results from a Spanish national survey on the application of ultrasound in pulmonology services
Authors: Ramos-Hernández Cristina , Botana-Rial Maribel , Cordovilla-Pérez Rosa , Núñez-Delgado Manuel , Fernández-Villar Alberto
Published: August 2021

Background

This was an observational, cross-sectional, and multicentre study carried out from October to December 2020, through a survey sent to Spanish Society of Pulmonology and Thoracic Surgery members in public hospitals with different levels of complexity. Our objective was to complete a national analysis of clinical practice, organisation, infrastructure, the services portfolio, teaching, and research activity related to ultrasound.

Results

Data from 104 hospitals were analysed. Ultrasound was used in 56.7% of cases, both in the area of bronchopleural techniques and on conventional wards, with no differences between centres. Lung ultrasound (LUS) was performed more often in the procedures area in intermediate-complexity centres compared to high- and low-complexity centres (36% vs. 31% and 6.25%, respectively). More high-complexity centres had three or more ultrasound scanners than intermediate-complexity centres (38% vs. 16%); 43% of low-complexity centres shared their ultrasound equipment with other specialties. Fewer than 6% of centres did not have an ultrasound machine. LUS was most often used during the treatment of pleural effusion (91.3%), in the differential diagnosis of dyspnoea (51.9%), and to rule out iatrogenic pneumothorax (50.9%). Only 5.7% of the centres had a pulmonologist specialised in LUS. Finally, fewer than 35% of the hospitals were teaching centres and fewer than 18% participated in research projects.

Conclusions

The use and availability of LUS has grown in pulmonology services, however, still relatively few pulmonologists are specialised in its use. Moreover, teaching and research activity in this field is scarce. Strategies are necessary to improve physicians’ skill at using LUS and to promote its use, with the ultimate goal of improving healthcare activity.
The hemodynamic effects of intravenous paracetamol (acetaminophen) in patients with chronic liver disease undergoing liver transplantation: a pilot study
Authors: Weinberg Laurence , Chiam Elizabeth , Karp Jadon , Churilov Leonid , Bellomo Rinaldo
Published: August 2021

Objective

We performed a single-center double-blinded, randomized trial to investigate the hemodynamic effects of IV paracetamol in patients with chronic liver disease (CLD) undergoing liver transplantation surgery. Patients with CLD are particularly susceptible to hemodynamic derangements given their low systemic vascular resistance state. Accordingly, hypotension is common in this setting. The hemodynamic effects of IV paracetamol in patients undergoing elective liver transplantation are unknown, therefore we evaluated whether the intraoperative administration of IV paracetamol in patients with chronic liver disease undergoing liver transplantation results in adverse hemodynamic effects. The primary end point was a change in systolic blood pressure 30-min after the preoperative infusion.

Results

Twenty-four participants undergoing liver transplantation surgery were randomly assigned to receive a single bolus of IV paracetamol (1 g paracetamol + 3.91 g mannitol per 100 mL) (n = 12) or placebo (0.9% Saline 100 mL) (n = 12). All participants completed their study intervention, and there were no breaches or violations of the trial protocol. Baseline characteristics were similar in both groups. There were no significant differences regarding surgical duration, intraoperative use of fluids, and intraoperative noradrenaline use. After the administration of paracetamol there were no significant differences observed in blood pressure or other hemodynamic parameters when compared to placebo.
Stability analysis for a class of implicit fractional differential equations involving Atangana–Baleanu fractional derivative
Authors: Asma  , Shabbir Sana , Shah Kamal , Abdeljawad Thabet
Published: August 2021

Some fundamental conditions and hypotheses are established to ensure the existence, uniqueness, and stability to a class of implicit boundary value problems (BVPs) with Atangana–Baleanu–Caputo type derivative and integral. The required results are established by utilizing the Banach contraction mapping principle and fixed point theorem of Krasnoselskii. In addition, various types of stability results including Hyers–Ulam, generalized Hyers–Ulam, Hyers–Ulam–Rassias, and generalized Hyers–Ulam–Rassias stability are formulated for the problem under consideration. Pertinent examples are given to justify the results we obtain.
Preliminary study of alcohol problem severity and response to brief intervention
Authors: Meredith Lindsay R. , Grodin Erica N. , Karno Mitchell P. , Montoya Amanda K. , MacKillop James , Lim Aaron C. , Ray Lara A.
Published: August 2021

Background

Findings have been mixed as to whether brief intervention (BI) is appropriate and effective for individuals with more severe alcohol use problems. Motivation to change drinking has been supported as a mechanism of behavior change for BI. This exploratory study examined aspects of motivation as mechanisms of clinical response to BI and alcohol problem severity as a moderator of treatment effects.

Methods

Non-treatment-seeking heavy drinkers (average age = 35 years; 57% male) were randomized to receive BI (n = 27) or attention-matched control (n = 24). Three indices of motivation to change were assessed at baseline and post-intervention: importance, confidence, and readiness. Moderated mediation analyses were implemented with treatment condition as the focal predictor, changes in motivation as mediator, 1-month follow-up drinks per day as the outcome, and an alcohol severity factor as second-stage moderator.

Results

Analysis of importance displayed a significant effect of intervention condition on importance (p < 0.003) and yielded a significant index of moderated mediation (CI − 0.79, − 0.02), indicating that the conditional indirect effect of treatment condition on drinking through importance was stronger for those with higher alcohol severity. For all motivation indices, alcohol severity moderated the effect of post-intervention motivation levels on drinking (p’s < 0.05). The direct effect of treatment condition on drinking was not significant in any model.

Conclusions

Findings highlight the relevance of considering one’s degree of alcohol problem severity in BI and alcohol screening efforts among non-treatment seeking heavy drinkers. These nuanced effects elucidate both potential mechanisms and moderators of BI response.

Trial registration Clinicaltrials.gov: NCT04710095. Registered January 14, 2021—retrospectively registered, https://clinicaltrials.gov/ct2/show/study/NCT04710095.
Exploring highly reliable substructures in auto-reconstructions of a neuron
Authors: He Yishan , Huang Jiajin , Wu Gaowei , Yang Jian
Published: August 2021

The digital reconstruction of a neuron is the most direct and effective way to investigate its morphology. Many automatic neuron tracing methods have been proposed, but without manual check it is difficult to know whether a reconstruction or which substructure in a reconstruction is accurate. For a neuron’s reconstructions generated by multiple automatic tracing methods with different principles or models, their common substructures are highly reliable and named individual motifs. In this work, we propose a Vaa3D-based method called Lamotif to explore individual motifs in automatic reconstructions of a neuron. Lamotif utilizes the local alignment algorithm in BlastNeuron to extract local alignment pairs between a specified objective reconstruction and multiple reference reconstructions, and combines these pairs to generate individual motifs on the objective reconstruction. The proposed Lamotif is evaluated on reconstructions of 163 multiple species neurons, which are generated by four state-of-the-art tracing methods. Experimental results show that individual motifs are almost on corresponding gold standard reconstructions and have much higher precision rate than objective reconstructions themselves. Furthermore, an objective reconstruction is mostly quite accurate if its individual motifs have high recall rate. Individual motifs contain common geometry substructures in multiple reconstructions, and can be used to select some accurate substructures from a reconstruction or some accurate reconstructions from automatic reconstruction dataset of different neurons.
Dental implants and risk of bleeding in patients on oral anticoagulants: a systematic review and meta-analysis
Authors: Dawoud Basim E. S. , Kent Samuel , Tabbenor Oliver , George Pynadath , Dhanda Jagtar
Published: August 2021

Background

Dental implant placement is safe and predictable, yet optimal management of anticoagulated patients remains controversial. Whilst cessation of anticoagulation pre-operatively should decrease risks of bleeding, risk of thrombosis increases. We aim to define risk of bleeding in patients on oral anticoagulation who are undergoing dental implant placement, in order to establish best management.

Methods

This systematic review is registered with the National Institute for Health Research (NIHR) PROSPERO database (Registration No: CRD42021233929). We performed a systematic review as per Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidance. Studies were identified using an agreed search strategy within the OVID Gateway (this included Pubmed, MEDLINE, Cochrane Collaborative). Studies assessing bleeding complications in patients who were undergoing dental implant placement were selected. The primary outcome was bleeding events in anticoagulated patients undergoing dental implant placement. Secondary outcomes included any complication requiring further intervention.

Results

We identified 182 studies through screening, and after review of titles and abstracts reduced this to 8 studies. In these studies, 1467 participants received at least 2366 implants. Studies were analysed for quality using the ROBINS-I risk of bias tool. Four studies were retrospective case reviews, and four were prospective reviews, three of which also blinded the operator to anticoagulation status. There was significant heterogeneity between the included studies. Meta-analysis showed an increased risk of bleeding (RR, 2.30; 95% CI, 1.25-4.24 p = 0.37 I = 7%) when implants were placed in the presence of anticoagulation however these were not clinically significant haemorrhagic events.

Conclusion

The continuation of anticoagulants peri-operatively during dental implant surgery does increase the risk of clinically non-significant peri- and post-operative bleeding. Dental implant surgery encompasses a broad spectrum of procedures ranging from minor to more invasive surgery with simple local haemostatic measures mitigating the risk of bleeding. The decision to discontinue anticoagulants prior to dental implant surgery must consider patient and surgical factors with the clinician undertaking a risk-balance assessment.
Fungi inhabiting attine ant colonies: reassessment of the genus Escovopsis and description of Luteomyces and Sympodiorosea gens. nov.
Authors: Montoya Quimi Vidaurre , Martiarena Maria Jesus Sutta , Bizarria Rodolfo , Gerardo Nicole Marie , Rodrigues Andre
Published: August 2021

Escovopsis is a diverse group of fungi, which are considered specialized parasites of the fungal cultivars of fungus-growing ants. The lack of a suitable taxonomic framework and phylogenetic inconsistencies have long hampered Escovopsis research. The aim of this study is to reassess the genus Escovopsis using a taxonomic approach and a comprehensive multilocus phylogenetic analysis, in order to set the basis of the genus systematics and the stage for future Escovopsis research. Our results support the separation of Escovopsis into three distinct genera. In light of this, we redefine Escovopsis as a monophyletic clade whose main feature is to form terminal vesicles on conidiophores. Consequently, E. kreiselii and E. trichodermoides were recombined into two new genera, Sympodiorosea and Luteomyces, as S. kreiselii and L. trichodermoides, respectively. This study expands our understanding of the systematics of Escovopsis and related genera, thereby facilitating future research on the evolutionary history, taxonomic diversity, and ecological roles of these inhabitants of the attine ant colonies.
Intraovarian platelet-rich plasma: current status
Authors: Elnashar Aboubakr Mohamed
Published: August 2021

Background

The successful use of PRP in regenerative medicine has led investigators to study its effect in the treatment of conditions like decreased ovarian reserve, poor responders, and premature ovarian insufficiency.

Objective

To review the uses of intraovarian PRP in reproductive medicine.

Methods

A literature search for English articles related to the uses of intraovarian PRP in reproductive medicine, including articles published in PubMed, from 2000 to April 2021.

Results

Seventeen studies: No RCT. Studies were classified according to indication. (1) For diminished ovarian reserve: 6 studies including 369 patients. (2). For poor responders: 3 case series studies including 41 patients with an improvement in ovarian response. (3) For premature ovarian insufficiency: 8 studies including 373 patients. Ovarian reserve markers were the outcome of all studies with conflicting results. LBR was not determined in the majority of studies.

Conclusion

Intraovarian PRP for diminished ovarian reserve, poor ovarian response, or POI is still experimental. Well-designed, large RCTS to confirm its efficacy and safety are required.
Molecular understanding of postharvest flower opening and senescence
Authors: Sun Xiaoming , Qin Meizhu , Yu Qin , Huang Ziwei , Xiao Yue , Li Yang , Ma Nan , Gao Junping
Published: August 2021

Flowers are key organs in many ornamental plants, and various phases of flower development impact their economic value. The final stage of petal development is associated with flower senescence, which is an irreversible process involving programmed cell death, and premature senescence of cut flowers often results in major losses in quality during postharvest handling. Flower opening and senescence are two sequential processes. As flowers open, the stamens are exposed to attract pollinators. Once pollination occurs, flower senescence is initiated. Both the opening and senescence processes are regulated by a range of endogenous phytohormones and environmental factors. Ethylene acts as a central regulator for the ethylene-sensitive flowers. Other phytohormones, including auxin, gibberellin, cytokinin, jasmonic acid and abscisic acid, are also involved in the control of petal expansion and senescence. Water status also directly influences postharvest flower opening, while pollination is a key event in initiating the onset flower senescence. Here, we review the current understanding of flower opening and senescence, and propose future research directions, such as the study of interactions between hormonal and environmental signals, the application of new technology, and interdisciplinary research.
A dual formulation of wavefield reconstruction inversion for large-scale seismic inversion
Authors: Rizzuti Gabrio , Louboutin Mathias , Wang Rongrong , Herrmann Felix J.
Published: August 2021

Many currently proposed seismic inversion techniques that focus on robustness with respect to the background model choice are not appropriate for large-scale 3D applications, and the methods that are computationally feasible for industrial problems, such as full-waveform inversion, are notoriously limited by convergence stagnation and require adequate starting models. We have developed a novel solution that is scalable and less sensitive to starting models or inaccurate parameters (such as anisotropy) that are typically kept fixed during inversion. It is based on a dual reformulation of the classic wavefield reconstruction inversion, whose empirical robustness with respect to these issues is well documented in the literature. Although the classic version is not suited to three dimensions because it leverages expensive frequency-domain solvers for the wave equation, our proposal allows the deployment of state-of-the-art time-domain finite-difference methods, and it is potentially sufficiently mature for industrial-scale problems.
Effects of diabase intrusions on surrounding clastic rock reservoir in early diagenetic stages: A case study of the Early Permian Fengcheng Formation in the Dabasong uplift, Junggar Basin
Authors: Chen Gangqiang , Liu Hailei , Tang Yuantao , Niu Zhijie , Yu Jing , Bian Baoli
Published: August 2021

A stage of mafic magmatic activity occurred in Early Permian in the Dabasong Uplift of Junggar Basin, part of the magma intruded into the normal sedimentary and shallow buried fine sandstone to form diabase, and part of the magma erupted to form basalt. The surrounding fine sandstone just entered in the early diagenetic stage A when the magma intruded. The compaction of the surrounding clastic rock and rupture of a small number of clastic grains were caused by the extrusion of the magma intrusion. The presence of chemically deposited alkaline minerals such as calcite, dolomite, shortite, natural alkali, and northupite indicates an alkali lake sedimentary environment for the Fengcheng Formation. Primary alkaline minerals dissolved from the surrounding rocks were subsequently transported and precipitated to form cements. The formation of the calcite cements and calcite metasomatism resulted in considerable densification of the surrounding rock during early diagenesis and destruction of the reservoir quality. The mafic magma had abundant Fe2+ and Mg2+ ions and was deficient in K+ ions, resulting in large amounts of chlorite and iron precipitation in the surrounding rock mainly composed of clay. We have analyzed the influence of an ultrashallow intrusion on the surrounding clastic rock during the early diagenetic period, which provided a typical reference for establishing a systematic mechanistic model of how magmatic intrusions affect the surrounding rock.
Extending subsurface imaging beyond ocean-bottom node coverage using multiples: A case study in deepwater Nigeria
Authors: Salami Rasaki , Lafram Abderrahim , Lecerf Didier , Asnaashari Amir
Published: August 2021

We have evaluated the results of a receiver decimation study in a deepwater context using separated wavefield imaging (SWIM) algorithms to provide extended illumination for imaging without ocean-bottom node (OBN) positioning constraints. We carried out subsurface imaging using the SWIM imaging technique with a reduced OBN layout, and we compared the results with those from conventional one-way wave-equation migration. We found from the results that the SWIM algorithm makes it possible to reduce the OBN layout while obtaining a similar subsurface image with the same shot geometry, which allows a reduced receiver acquisition effort, offers more geometry flexibility without affecting the image quality, with a potentially significant reduction of acquisition cost and 4D processing turnaround time.
Attribute analyses of acoustic emissions in hydraulic fracturing
Authors: Acosta Juan Camilo , Dang Son T. , Sondergeld Carl H. , Rai Chandra S.
Published: August 2021

Hydraulic fracturing (HF) and horizontal drilling are essential to the development of shale gas and oil. Production depends on the stimulation success. During fracture initiation, propagation, and closure, cracks emit acoustic waves; these can be monitored in real time as microseismics in the field and as acoustic emissions (AEs) in the laboratory. AEs are the laboratory equivalent of field-scale microseismics and contain detailed information about HF fracture mechanics. The number of acoustic events correlates with the number of induced fractures and hence the stimulation volume. Three HF protocols under dry conditions were carried out on Tennessee sandstone: (1) a constant injection rate, (2) a precyclic injection, and (3) a variable-rate injection test. All three tests were performed under the same principal stress conditions: vertical stress of 10.3 MPa (1500 psi), minimum horizontal stress of 3.5 MPa (500 psi), and maximum horizontal stress of 20.7 MPa (3000 psi). In total, 16 piezoelectric transducers were mounted around a cylindrical sample to record the AEs. We have performed postsignal processing to extract AE event attributes, including the amplitudes, signal-to-noise ratio, arrival time, event location (with the velocity-anisotropy input), and frequency analyses. The AE events associated with the constant-rate injection test possessed the lowest frequencies (150–270 kHz). The variable-rate test AE events possessed higher frequencies (160–310 kHz), whereas the precyclic injection had events with the highest frequencies, peaking at 330 kHz. Acoustic events before failure had lower amplitudes, but higher frequency compared to those recorded postbreakdown, suggesting different failure modes. Precyclic injection induced the greatest number of locatable events before and after failure.
kNN-based gas-bearing prediction using local waveform similarity gas-indication attribute — An application to a tight sandstone reservoir
Authors: Song Zhaohui , Yuan Sanyi , Li Zimeng , Wang Shangxu
Published: August 2021

Gas-bearing prediction of tight sandstone reservoirs is significant but challenging due to the relationship between the gas-bearing property and its seismic response being nonlinear and complex. Although machine learning (ML) methods provide potential for solving the issue, the major challenge of ML applications to gas-bearing prediction is that of generating accurate and interpretable intelligent models with limited training sets. The k nearest neighbor ( kNN) method is a supervised ML method classifying an unlabeled sample according to its k neighboring labeled samples. We have introduced a kNN-based gas-bearing prediction method. The method can automatically extract a gas-sensitive attribute called the gas-indication local waveform similarity attribute (GLWSA) combining prestack seismic gathers with interpreted gas-bearing curves. GLWSA uses the local waveform similarity among the predicting samples and the gas-bearing training samples to indicate the existence of an exploitable gas reservoir. GLWSA has simple principles and an explicit geophysical meaning. We use a numerical model and field data to test the effectiveness of our method. The result demonstrates that GLWSA is good at characterizing the reservoir morphology and location qualitatively. When the method applies to the field data, we evaluate the performance with a blind well. The prediction result is consistent with the geologic law of the work area and indicates more details compared to the root-mean-square attribute.