bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGARUH ABSENTEEISM DAN JOB ATTITUDE TERHADAP KINERJA KARYAWAN PADA PERUSAHAAN LEASING DI KOTA PADANG
Authors: Lukito Hendra , Utama M Kurnia
Published: December 2019

This study aims to examine and analyze the effect of absenteeism and job attitude on employee performance in leasing companies in Padang City, West Sumatra Province. Absenteeism defined as the absenteeism of employees from their work responsibility. Job attitude can be measured through job satisfaction and organizational commitment. The population was all employees who work at one of the leasing companies located in Padang.  The sampling method used was saturated sampling (census). Research data was collected by distributing questionnaires to a total of 43 respondents. Data processing and analysis were performed using a multiple linear regression method run by SPSS version 22.0. The results of the study indicated that absenteeism has no significant effect on employee performance. Job satisfaction, as part of job attitude, had a significant effect on employee performance, while organizational commitment did not significantly affect performance.
School of self-education reading as a mean of training teacher for professional activities
Authors: Pervova Galina
Published: December 2019

At the Derzhavin Tambov State University, on the basis of which the regional branch of the Russian Reading Association was opened (the head of the local branch is Doctor of Sociology, professor of Derzhavin Tambov State University N.A. Stefanovskaya), relevant project “School of self-education reading” has been implemented for several years. Classes at this school are a necessity for all university employees: the project meets the needs of students’ academic work, research and pedagogical practice of graduate students and teachers, as well as intellectual amateur inquiries in the acquisition of cognitive and fiction literature for everyone who wants to improve reading skills. We disclose the experience of organizing work with student readers at the Theory and Methods of Pre-School and Elementary Education Department of Derzhavin Tambov State University. We reveal means and methods of reading activity of future teachers, the issues of reading circle formation and reader’s interests. We pay particular attention to the study of the motivation development of children and teenagers to read, which is one of the main skills of the future teacher. We name the sources of modern literary education of students and their teachers. The conclusions of the article are related to the transformation of educational reading into the process of improving the reading culture at all levels of reading.
Problematic aspects of personal and professional adaptation of university students
Authors: Sharshov Igor , Koroleva Аnna , Prokudin Yuri
Published: December 2019

We consider the current problems arising in the process of first year students professionalization. Based on contemporary educational standards and the basic provisions of the competency-based approach, we substantiate the necessity of special work with first year students at the stage of their entry into the profession in such areas as: understanding personal characteristics, development of skills to use their own resources and reserves; disclosure of the psychological con-text of choice and further development within the profession; prevention of the negative conse-quences of professionalization; the use of recreational tools to maintain psychological and social health; development of a positive life scenario, taking into account professional characteristics. We identify contradictions in accompanying the process of personal and professional adaptation of students in the considered areas. We substantiate the necessity of developing a special technology focused on resolving the identified contradictions.
Relación entre la procrastinación académica y ansiedad-rasgo en estudiantes universitarios pertenecientes al primer año de estudios de una universidad privada de Lima Metropolitana.
Authors: Mamani Guerra Susana Elizabeth , Aguilar Angeletti Ana
Published: December 2019

La finalidad de la presente investigación fue determinar si existe relación entre la procrastinación académica y la ansiedad rasgo en 200 estudiantes universitarios pertenecientes al primer año de estudios de una universidad privada de Lima Metropolitana. De igual manera, se determinó la correlación entre las dimensiones de la procrastinación académica; autorregulación académica y postergación de actividades con ansiedad rasgo. Además, se realizó un análisis comparativo de la procrastinación académica en relación a la edad, sexo, composición familiar, lugar de procedencia, facultad y número de cursos desaprobados. Se utilizó la Escala de Procrastinación Académica y el Inventario de Ansiedad Rasgo-Estado. Se encontró correlaciones de tipo muy débil entre la procrastinación académica y ansiedad rasgo (r=.068), autorregulación académica y ansiedad rasgo (r=.152) y postergación de actividades y ansiedad rasgo (r=.158). No se encontró diferencias significativas según las variables de comparación en la dimensión de postergación de actividades, en la dimensión de autorregulación académica sólo se halló diferencias significativas según sexo.
ULAŞTIRMA SEKTÖRÜNÜN FİNANSAL PERFORMANS ÖLÇÜMÜ İÇİN TOPSIS VE PROMETHEE YÖNTEMLERİNİN KULLANILMASI: BIST ÜZERİNE BİR UYGULAMA
Authors: ORAL Ceren , KIPKIP Elçin
Published: December 2019

Günümüzde ulaşım uluslararası boyutta olup, toplumsal, sosyal ve ekonomik açıdan
değerlendirilebilmektedir. Ulaştırma sektörü, ekonomik kaynak kullanımı, dış ülkeler ile
bağlantının sağlanabilmesi, mal akışlarının düzenli olması ve ulaştırma sürecinin sürekliliğinin
devamı, mevcut bulunan ya da yeni yerleşim alanlarının gelişebilmesi açısından önem arz
etmektedir.Bu çalışmada, BIST’de yer alan sekiz ulaştırma şirketinin 2014-2018 yıllarına ait beş
yıllık finansal tabloları esas alınarak TOPSIS ve PROMETHEE yöntemleri yardımıyla finansal
performansları ölçülmüştür. Bu şirketlerin finansal performans durumları ortaya konulmuştur.
ÇEVRE BİLİNCİ, ÇEVRECİ EV UYGULAMALARI, SATIN ALMA DAVRANIŞI VE DEMOGRAFİK FAKTÖRLER ARASINDAKİ İLİŞKİNİN HANELER KAPSAMINDA İNCELENMESİ
Authors: BÜLBÜL Hasan , BÜYÜKKEKLİK Arzum , ÖZOĞLU Buket
Published: December 2019

Küresel ısınma ve beraberinde gelişen iklim değişiklikleri pek çokları tarafından dünya
toplumlarının yüz yüze olduğu en büyük problem olarak kabul edilmektedir. Bu problemle
mücadelede kurumlar kadar bireylere de önemli görevler düşmektedir. Alışveriş davranışları,
günlük ev uygulamaları ve yaşam tarzları ile ilgili konular çevre kirliliğine neden olmakta ve sera
gazı salınımını artırmaktadır. Bu çalışmada, Niğde ilindeki 403 haneden elde edilen veriler
kullanılarak, çevre bilinci ile çevreci ev uygulamaları ve satın alma davranışı arasındaki ilişkiye
ve ebeveynlerin sosyoekonomik özelliklerinin bu bağlantıya etkisine dair bulgular sunulmuştur.
Çevre bilincinin bir boyutunu oluşturan farkındalığın anne ve babanın eğitimi ile arttığı, buna
karşın çevreci davranışlar için eğitimin yeterli olmadığı sonucuna ulaşılmıştır. Benzer sonuç hane
geliri bakımından da ortaya çıkmıştır. Çalışmada elde edilen bulgular bir bütün olarak
değerlendirildiğinde, çevre bilincinin farkındalık boyutunun, hanelerde çevreci uygulamaların
gerçekleştirmesinde yeterli olmadığı, hanelerin ancak özümseme (benimseme) düzeyinde çevre
bilincine ulaşması ile çevre sorunlarının azaltılmasına destek verebileceği sonucuna ulaşılmıştır.
STRATEGI KOMUNIKASI PERHIMPUNAN BATIK INDONESIA SOKARAJA DALAM MEMBANGUN CITRA “KAMPUNG BATIK SOKARAJA”
Authors: Trifosa Sabrina Sesa , Wijaya Lina Sinatra
Published: December 2019

Penelitian ini bertujuan untuk mengetahui mengetahui strategi komunikasi dan peran yang dilakukan oleh Perhimpunan Batik Indonesia (PERBAIN) Sokaraja dalam membangun citra “Kampung Batik Sokaraja”. Jenis penelitian ini adalah penelitian deskriptif kualitatif. Penelitian ini dilaksanakan di Kampung Batik Sokaraja, Kabupaten Banyumas, Jawa Tengah. Pengumpulan data dilakukan melalui observasi, wawancara, dan   data   kepustakaan.   Strategi   yang   diterapkan   Perhimpunan   Batik   Indonesia (PERBAIN) Sokaraja dalam membangun citra Kampung Batik Sokaraja yakni mengadakan workshop pelatihan kepada pengrajin batik dan masyarakat sekitar, merencanakan program jangka pendek dan panjang seperti mengadakan diskusi dan pengarahan, meluncurkan program inovasi batik, menjadikan kampung batik Sokaraja sebagai  tempat  wisata  batik.  Perhimpunan  Batik  Indonesia  (PERBAIN)  Sokaraja berperan sebagai communication technician dengan menyediakan layanan website dan Instagram untuk mengenalkan Kampung Batik Sokaraja, lalu sebagai expert preciber dalam   memberdayakan   Kampung   Batik   Sokaraja   sebagai   peningkatan   ekonomi, kemudian sebagai communication facilitator dalam menggerakkan masyarakat untuk melestarikan  batik  Sokaraja,  dan  sebagai  problem  solver  dengan  membentuk  forum curhat bisnis.
The Implementation of Iron Supplementation and Antenatal Counseling for Iron Deficiency Anemia in Pregnancy
Authors: Darmawati Darmawati , Tahlil Teuku , Siregar Toungku Nizwan , Kamil Hajjul , Audina Masyithah
Published: December 2019

Anemia is one of the contributing conditions that cause maternal mortality in Indonesia. The Indonesian Ministry of Health try to reduce it through an antenatal care program conducted at public health center, with several indicators including iron supplementation and antenatal counseling. This study was conducted to find out the relationship between iron supplementation and antenatal counseling to iron deficiency anemia during pregnancy. This study was a correlative study with cross sectional approach carried out in February until June 2018. The population was 1,285 pregnant women with 102 samples recruited using purposive sampling technique. Blood hemoglobin levels were examined using Hb Meter. Data collection were carried out through guided interviews. Data analysis was done using Chi-Square Test. The implementation of iron supplementation and antenatal counseling was implemented comprehensively (51.0%; 64.7% respectively). This study showed that there was a relationship between iron supplementation and iron deficiency anemia (0.000) and there was no relationship between antenatal counseling and iron deficiency anemia (0.249). It is recommended to all antenatal providers to improve the service provided, especially the iron supplementation that accompanied by giving information through antenatal counseling, so the high maternal mortality rates due to iron deficiency anemia can be treated immediately.
Caryophyllene-Type Sesquiterpenoids from the Stembark of Aglalia harmsiana and Their Cytotoxic Activity Against MCF-7 Breast Cancer Cells
Authors: Milawati Hersa , Harneti Desi , Maharani Rani , Nurlelasari Nurlelasari , Hidayat Ace Tatang , Azmi Mohamad Nurul , Shiono Yoshihito , Supratman Unang
Published: December 2019

Sesquiterpenoid is a class of terpenoid compounds that have the most abundant diversity of structures and biological activitiesthat can be found in natural resources. Tropical plants are main source of sesquiterpenoid compounds such as Aglaiagenus belong to Meliaceae family. A. harmsianais a species from Aglaiathat only has few previous researchs.  Therefore, the purpose of this study was to isolate and determine the structure of sesquiterpenoid compounds from stem barkA. harmsianaalong withtheir cytotoxic activity against MCF-7 breast cancer cells. The isolation process begins by extracting powder from A. harmsianastembark using n-hexane, ethyl acetate and methanol. All extracts were evaluated for their cytotoxic activity against MCF-7 breast cancer cells, and n-hexane extractsshowed significant cytotoxic activitywith IC50values of 117.86 µg/mL. Therefore, n-hexane extracts were further separated and purified by various chromatographic techniquesto obtain compounds 1and 2. Compounds 1and 2were elucidated their chemical structures by spectroscopic methods includingIR, NMR, and MS as well as bycomparison of data with literatures and identified ascaryophyllene-typesesquiterpenoids, β-caryophylleneoxide (1) and senecrassidiol (2). Compounds 1and 2were submitted for cytotoxic eveluation on MCF-7 breast cancer cells and as a result, β-caryophyllene oxide (1) showed the stronger activity compared to senecrassidiol (2). These finding indicatedthat the cytotoxic activity of caryophyllene-typesesquiterpenoid areinfluenced by the presence of double bonds and configuration of methyl groups.
Exploring Mammalian Genome within Phase-Separated Nuclear Bodies: Experimental Methods and Implications for Gene Expression
Authors: Lesne Annick , Baudement Marie-Odile , Rebouissou Cosette , Forné Thierry
Published: December 2019

The importance of genome organization at the supranucleosomal scale in the control of gene expression is increasingly recognized today. In mammals, Topologically Associating Domains (TADs) and the active / inactive chromosomal compartments are two of the main nuclear structures that contribute to this organization level. However, recent works reviewed here indicate that, at specific loci, chromatin interactions with nuclear bodies could also be crucial to regulate genome functions, in particular transcription. They moreover suggest that these nuclear bodies are membrane-less organelles dynamically self-assembled and disassembled through mechanisms of phase separation. We have recently developed a novel genome-wide experimental method, High-salt Recovered Sequences sequencing (HRS-seq), which allows the identification of chromatin regions associated with large ribonucleoprotein (RNP) complexes and nuclear bodies. We argue that the physical nature of such RNP complexes and nuclear bodies appears to be central in their ability to promote efficient interactions between distant genomic regions. The development of novel experimental approaches, including our HRS-seq method, is opening new avenues to understand how self-assembly of phase separated nuclear bodies possibly contributes to mammalian genome organization and gene expression.
Improving Quality of Service in Cloud Computing
Authors: Sajid Sobia , Ahmed Sadia , Waheed Arooj , Sirshar Mehreen
Published: December 2019

Quality of Service (QoS) has a significant role in the provision of resources within service oriented distributed systems. In Quality of Service, cloud computing creates new challenges for improvements using the concept of virtualization. Currently, Cloud Computing is very emerging technology in every field of data storage and resource distribution over the network. Considering this new emerging technology, for the ease of data accessibility, price, resource use, restoration, response time and number of constraints the quality performance measures need to be upgraded. The paper highlights the research gap in providing a solution to achieve a Quality of Services in Cloud Computing. We also review the issues and challenges arising in cloud computing to guarantee quality.
Nuclear Export of Cyclin B Mediated by the Nup62 Complex is Required for Meiotic Initiation in Drosophila Males
Authors: Okazaki Ryotaro , Yamazoe Kanta , Inoue Yoshihiro H.
Published: December 2019

Background: The central channel of the nuclear pore complex (NPC) plays an important role in the selective transport of proteins between the nucleus and cytoplasm. Previous studies have demonstrated that depletion of the Nup62 complex, constructing the nuclear pore channel in premeiotic Drosophila cells, resulted in absence of meiotic cells. We attempted understanding the mechanism underlying the cell cycle arrest before meiosis. Methods: We induced dsRNAs against the nucleoporin mRNAs using the Gal4/UAS system in Drosophila. Results: The cell cycle of the Nup62-depleted cells was arrested before meiosis without CDK1 activation. Ectopic over-expression of CycB, but not constitutively active CDK1, resulted in partial rescue from the arrest. CycB accumulated precociously in the nuclei of Nup62-depleted cells and cells depleted of exportin (encoded by emb). Protein complexes containing CycB, Emb, and Nup62 complex components were observed in premeiotic spermatocytes. CycB, which had precociously migrated into the nucleus, was associated with Emb, and the complex was transported back to the cytoplasm through the central channel, interacting with the Nup62 complex. Conclusion: We proposed that CycB is exported with Emb through the channel interacting with the Nup62 complex before initiation of meiosis. The nuclear export ensures production of active CycB-CDK1 in the cytoplasm.
Elaboración de modelos anatómicos mediante técnicas de plastinación como alternativa al uso de animales en docencia y como recurso esencial en el proceso de enseñanza-aprendizaje
Authors: Molina López Ana María , Diz Plaza Andrés , Ayala Soldado Nahúm , Diz Conde Lorena , Lora Benítez Antonio , Martínez Galisteo Alfonso , Vivo Rodríguez Joaquín , Navas Lloret José Miguel , Barasona Carmona María Isabel , Bujalance Reyes Francisca , Rueda Jiménez Andrés , Fernández Díez Ana , Montero Pérez-Barquero Estrella , Moyano Salvago Rosario
Published: December 2019

La implantación del principio de las 3Rs (Reducción, Refinamiento y Reemplazo), descritas por Russell y Burch en 1959, fue el punto de partida de numerosos cambios en la utilización de los animales, tanto en el ámbito de la investigación como de la docencia. Por tanto, es necesario ir propiciando el empleo de alternativas y que el profesorado disponga de recursos y de una buena información. Este proyecto se propone como objetivo esencial la elaboración de modelos animales mediante técnicas de plastinación como recurso docente alternativo al uso de animales vivos, y proporcionando al profesorado la disposición de medios y recursos esenciales en el proceso de enseñanza-aprendizaje del alumnado.Los alumnos podrán disponer de una variedad de modelos animales en las sesiones prácticas, y/o teórico/prácticas de las distintas disciplinas en las que sea necesario el uso de animales. Podrán trabajar con un modelo fiel al original sin necesidad de utilizar modelos vivos y podrán desarrollar las distintas técnicas y procedimientos que realizarían in vivo sin necesidad de generar un estrés innecesario a los animales por su manejo por personal aún no capacitado para ello, evitando situaciones que hagan peligrar el bienestar animal (RD 53/2003). El desarrollo de estos modelos plastinados supondrá recurso docente esencial para la adquisición de las habilidades específicas en distintas disciplinas Biomédicas que conlleven el uso de animales, tales como los Grados en Veterinaria, Biología, Medicina, Enfermería, etc.
Introducción al modelado de información de construcción (BIM) en edificaciones e infraestructuras de ingeniería
Authors: Pérez Galvín Adela , Barbudo Muñoz Auxiliadora , Ayuso Muñoz Jesús , García Beltrán Manuel , Rosales García Julia , López Uceda Antonio
Published: December 2019

La actual exigencia técnica del manejo de programas de diseño en el ámbito de la Ingeniería Civil y la Edificación lleva a plantear de forma indispensable la necesidad de formación de los futuros profesionales en la metodología BIM (Building Information Modeling).Mediante el presente Proyecto de Innovación Docente se ha desarrollado un modelo de implantación de esta metodología adaptándolo al programa actual de cada asignatura y a las competencias exigidas. La metodología y conocimientos teóricos y prácticos de las herramientas BIM han sido impartidos a 40 alumnos aproximadamente pertenecientes a tres títulos y niveles universitarios diferentes. Los resultados obtenidos muestran una evaluación satisfactoria por parte de los alumnos, respondiendo mediante la enseñanza de nuevas herramientas de diseño a las crecientes necesidades profesionales y enriqueciendo las habilidades de los estudiantes universitarios.
Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco
Authors: Yao Wenjing , Zhang Dawei , Zhou Boru , Wang Jianping , Li Renhua , Jiang Tingbo
Published: December 2019

Background NAC (NAM/ATAF/CUC) is one of the largest plant-specific transcription factor (TF) families known to play significant roles in wood formation. Acting as master gene regulators, a few NAC genes can activate secondary wall biosynthesis during wood formation in woody plants. Results In the present study, firstly, we screened 110 differentially expressed NAC genes in the leaves, stems, and roots of di-haploid Populus simonii×P. nigra by RNA-Seq. Then we identified a nucleus-targeted gene, NAC15 gene, which was one of the highly expressed genes in the stem among 110 NAC family members. Thirdly, we conducted expression pattern analysis of NAC15 gene, and observed NAC15 gene was most highly expressed in the xylem by RT-qPCR. Moreover, we transferred NAC15 gene into tobacco and obtained 12 transgenic lines overexpressing NAC15 gene (TLs). And the relative higher content of hemicellulose, cellulose and lignin was observed in the TLs compared to the control lines containing empty vector (CLs). It also showed darker staining in the culms of the TLs with phloroglucinol staining, compared to the CLs. Furthermore, the relative expression level of a few lignin- and cellulose-related genes was significantly higher in the TLs than that in the CLs. ConclusionsThe overall results indicated that NAC15 gene is highly expressed in the xylem of poplar and may be a potential candidate gene playing an important role in wood formation in transgenic tobacco.
Prevalence and pattern of long acting reversible contraception (LARC) methods use in immediate postpartum period at Jimma University Medical Center, Ethiopia
Authors: Arero Wariyo Duba , Teka Woubishet Girma , Hebo Habtemu Jarso
Published: December 2019

Background: Postpartum family planning (PPFP) is defined as the prevention of unintended pregnancy and closely spaced pregnancies through the first twelve months following childbirth. The immediate postpartum period is particularly favorable time to provide long-acting reversible contraception (LARC) methods and postpartum provision of LARC methods is safe and effective. Despite the advantages of LARC methods, they are infrequently used in Ethiopia.
Objective: This study assessed the prevalence and pattern of LARC methods use among counseled mothers in immediate postpartum.
Methods: A cross-sectional study was conducted on 393 women who gave birth at Jimma University Medical Centre from 12 November 2016 to 21 January 2017. Data were collected by face-to-face interview and record review using pre-tested questionnaire and analysed using SPSS 20. Logistic regression was used to identify associated factors for LARC methods use.
Results: Prevalence of LARC methods use among immediate postpartum mothers was 53.2% (209/393) and more than three-fourths (78.0%) of participants used implanon. The most common reported reason for not using LARC methods was preference of other method (25.5%). Having more than four alive kids (95% CI: 1.15,5.95), high monthly income (≥1000 ETB) (95% CI: 1.08,7.20), planning to delay next pregnancy by more than 2 years (95% CI: 1.60,9.28), completed family size (95% CI: 1.12,3.15), prior use of LARC methods (95% CI: 1.30,7.20) and receiving counseling during antenatal care (ANC) follow-up and before delivery (95% CI: 1.01, 4.73) were associated with immediate postpartum LARC methods use.
Conclusion: Although the prevalence of LARC methods use in immediate postpartum was good, counseling mothers during ANC follow-up and before delivery can further increase its use. Therefore, the need for providing counseling during ANC follow up and before delivery to increase utilisation of immediate postpartum LARC methods use is emphasised.
Key terms: Long acting reversible contraception (LARC) methods, Immediate postpartum, Ethiopia
Using the anterior capsule of the hip joint to protect the tensor fascia lata muscle during direct anterior total hip arthroplasty: a randomized prospective trial
Authors: Zhao Gongyin , Zhu Ruixia , Jiang Shijie , Xu Nanwei , Bao Hongwei , Wang Yuji
Published: December 2019

Background

The direct anterior approach for total hip arthroplasty (THA) has specific advantages, but injury to the tensor fasciae lata muscle (TFLM) remains a concern. This injury in part negates some of the advantages of the intermuscular approach, because injury of the muscle fibers of the TFLM can lead to less satisfactory clinical results. Thus, in this study, we propose an intraoperative method to protect the TFLM and demonstrate its feasibility.Methods

Fifty-six patients undergoing THA by the direct anterior approach were divided randomly into two groups. In group A, the TFLM was protected by an autogenous tissue “pad” created from the anterior capsule of the joint that was rotated to protect the TFLM from direct contact with the retractors needed to expose the joint space. In group B, the operation was carried out in the typical fashion with no other protection of the TFLM except the attempt by the surgeons to consciously avoid compressive injury of the TFLM. We evaluated the magnitude of changes in the muscle cross-sectional area (MSCA) and fatty atrophy (FA) early and postoperatively between groups by magnetic resonance imaging. The differences in blood hemoglobin and serum levels of myoglobin, lactate dehydrogenase (LDH), and creatine phosphokinase (CPK) were compared at 8, 24, and 48 h, postoperatively. Postoperative drainage volume and pain evaluated by visual analogue scores (VAS) were compared between the two groups.Results

LDH, CPK and myoglobin in group B were significantly higher than those in group A at 8, 24, and 48 hours after the surgery. (p<0.05) Compared to the group A, the decrease of hemoglobin in group B displayed significantly at 24 and 48 hours after surgery. (P<0.05) The significantly increased MSCA and FA of TFLM were demonstrated in group B. The PDV and VAS in group B were significantly higher compared with those in group A. (P<0.05)Conclusions

Using the anterior capsule of the hip joint as an autogenous, protective capsular tissue pad to limit the trauma to the TFLM during a direct anterior approach to THA is an effective method to protect the TFLM and improve the clinical effect.
Prognostic Significance of Stem Cell/ Epithelial-Mesenchymal Transition Markers in Periampullary/Pancreatic Cancers: FGFR1 Is A Promising Marker.
Authors: Chong Yosep , Thakur Nishant , Paik Kwang Yeol , Lee Eun Jung , Kang Chang Suk
Published: December 2019

Background: Periampullary cancers (PAC) including pancreatic, ampulla of Vater (AOV), and common bile duct (CBD) cancers are highly aggressive with a lack of useful prognostic markers beyond T stage. However, T staging can be biased due to the anatomic complexity of this region. Recently, several markers related to cancer stem cells and epithelial-mesenchymal transition (EMT) such as octamer transcription factor-4 (Oct4) and fibroblast growth factor receptor 1 (FGFR1) respectively, have been proposed as new promising markers in other solid cancers. The aim of this study was to assess the expression and prognostic significance of stem cell/EMT markers in PACs. Methods : Formalin-fixed, paraffin-embedded tissues of surgically excised PACs from the laboratory archives from 1998 to 2014 were evaluated by immunohistochemical staining for stem cell/EMT markers using tissue microarray. The clinicopathologic parameters were documented and statistically analyzed with the immunohistochemical findings. Survival and recurrence data were collected and analyzed. Results: A total of 126 PAC cases were evaluated. The average age was 63 years, with 76 male and 50 female patient samples. Age less than 74 years, AOV cancers, lower T & N stage, lower tumor size, no lymphatic, vascular, perineural invasion and histologic well differentiation, intestinal type, no fibrosis, severe inflammation were significantly associated with the better overall survival High expression levels of FGFR1 as well as CK20, CDX2, and VEGF were significantly related to better overall survival, while other stem cell markers were not related. Similar findings were observed for tumor recurrence using disease-free survival. Conclusions: In addition to other clinicopathologic parameters, severe fibrosis was related to frequent tumor recurrence, and high FGFR1 expression was associated with better overall survival. Histologic changes such as extensive fibrosis need to be investigated further in relation to EMT of PACs.
Gene signature characteristic of elevated stromal infiltration and activation is associated with increased risk of hematogenous and lymphatic metastasis in serous ovarian cancer
Authors: Yue Huiran , Wang Jieyu , Chen Ruifang , Hou Xiaoman , Li Jun , Lu Xin
Published: December 2019

Background

The clinical significance of hematogenous and lymphatic metastasis in ovarian cancer has been increasingly addressed, as it plays an imperative role in the formation of both intraperitoneal and distant metastases. Our objective is to identify the key molecules and biological processes potentially related to this relatively novel metastatic route in serous ovarian cancer.Methods

Since lymphovascular space invasion (LVSI) is considered as the first step of hematogenous and lymphatic dissemination, we developed a gene signature mainly based on the transcriptome profiles with available information on LVSI status in the Cancer Genome Atlas (TCGA) dataset. We then explored the underlying biological rationale and prognostic value of the identified gene signature using multiple public databases.Results

We observe that primary tumors with increased risk of hematogenous and lymphatic metastasis highly express a panel of genes, namely POSTN, LUM, THBS2, COL3A1, COL5A1, COL5A2, FAP1 and FBN1. The identified geneset is characterized by enhanced deposition of extracellular matrix and extensive stromal activation. Mechanistically, both the recruitment and the activation of stromal cells, especially fibroblasts, are closely associated with lymphovascular metastasis. Survival analysis further reveals that the elevated expression of the identified genes correlates to cancer progression and poor prognosis in patients with serous ovarian cancer.Conclusions

Our findings indicate that tumor stroma supports the hematogenous and lymphatic spread of ovarian cancer, increasing tumor invasiveness and ultimately resulting in worse survival. Thus stroma-targeted therapies may improve the clinical outcomes in combination with cytoreductive surgery and chemotherapy.
Working with patients suffering from chronic diseases – a balancing act for health care professionals. A meta-synthesis of qualitative studies
Authors: Holmen Heidi , Larsen Marie Hamilton , Sallinen Merja Helena , Thoresen Lisbeth , Ahlsen Birgitte , Andersen Marit Helen , Borge Christine Råheim , Eik Hedda , Wahl Astrid Klopstad , Mengshoel Anne Marit
Published: December 2019

Background: The number of patients with long-term chronic diseases is increasing. These patients place a strain on health care systems and health care professionals (HCPs). Presently, we aimed to systematically review the literature on HCPs’ experiences working with patients with long-term chronic diseases such as type 2 diabetes, chronic obstructive pulmonary disease (COPD), and chronic kidney disease (CKD). Method: A systematic search of papers published between 2002 and July 2019 was conducted in the Embase, AMED, PsycINFO, MEDLINE, CINAHL, and COCHRANE databases to identify studies reporting qualitative interviews addressing HCPs’ experiences working with adults with COPD, CKD or type 2 diabetes. An interdisciplinary research group were involved in all phases of the study. With the help of NVivo, extracts of each paper were coded, and codes were compared across papers and refined using translational analysis. Further codes were clustered in categories that in turn formed overarching themes. Results : Our comprehensive search identified 4170 citations. Of these, 20 papers met our inclusion criteria. Regarding HCPs’ experiences working with patients with COPD, CKD, or type 2 diabetes, we developed 10 sub-categories that formed three overarching main themes of work experiences: 1) individualizing one’s professional approach within the clinical encounter; 2) managing one’s emotions over time; 3) working to maintain professionalism. Overall these three themes suggest that HCPs’ work is a complex balancing act depending on the interaction between patient and professional, reality and professional ideals, and contextual support and managing one’s own emotions. Conclusion: Few qualitative studies highlighted HCPs’ general working experiences, as they mainly focused on the patients’ experiences or HCPs’ experiences of using particular clinical procedures. This study brings new insights about the complexity embedded in HCPs’ work in terms of weighing different, often contrasting aspects, in order to deliver appropriate practice. Acknowledging, discussing and supporting this complexity can empower HCPs to avoid burning out. Leaders, health organizations, and educational institutions have a particular responsibility to provide HCPs with thorough professional knowledge and systematic support.
Plasmodium vivax severe imported malaria in two migrants in France
Authors: Izri Arezki , Cojean Sandrine , Leblanc Claire , Cohen Yves , Bouchaud Olivier , Durand Remy
Published: December 2019

Background
With less than one severe case per year in average, Plasmodium vivax is very rarely associated with severe imported malaria in France. Two cases of P. vivax severe malaria occurred in patients with no evident co-morbidity. Interestingly, both cases did not occur at the primary infection but during relapses.
Case presentations
Patient 1: A 27-year old male, born in Afghanistan and living in France since 2012, was admitted on August 2015 to the Avicenne hospital because of abdominal pain, intense headache, fever and hypotension. The patient was haemodynamically unstable despite 5litres of filling solution. A thin blood film showed P. vivax trophozoites within the red blood cells. To take care of the septic shock, the patient was given rapid fluid resuscitation, norepinephrine (0.5 mg/h), and intravenous artesunate. Nested polymerase chain reactions of the SSUrRNA gene were negative for Plasmodiumfalciparum but positive for P. vivax. The patient became apyretic in less than 24H and the parasitaemia was negative at the same time.
Patient 2: A 24-year old male, born in Pakistan and living in France, was admitted on august 2016 because of fever, abdominal pain, headache, myalgia, and nausea. The last travel of the patient in a malaria endemic area occurred in 2013.A thin blood film showed P. vivax trophozoites within the red blood cells. The patient was treated orally by dihydroartemisinin-piperaquine and recovered rapidly. Nine months later, the patient returned to the hospital with a relapse of P. vivax malaria. The malaria episode was uncomplicated and the patient recovered rapidly. Three months later, the patient came back again with a third episode of P. vivax malaria. Following a rapid haemodynamic deterioration, the patient was transferred to the intensive care unit of the hospital. In all the patient received 10 litres of filling solution to manage the septic shock. After 5 days of hospitalization and a specific treatment, the patient was discharged in good clinical conditions.
Conclusion
Clinicians should be aware of the potential severe complications associated with P. vivax in imported malaria, even though the primary infection is uncomplicated.
Surgery Outcomes of Lamellar Macular Eyes with or without Lamellar Hole-Associated Epiretinal Proliferation: A Meta-Analysis
Authors: xu hanyue , Qin Ling , Zhang Yifan , Zhang Ming
Published: December 2019

Purpose: To compare the visual outcomesand rate of restored ellipsoid zone (REZ) after surgery of lamellar macular hole (LMH) with and without lamellar hole-associated epiretinal proliferation (LHEP). To determine whether all patients with LMH need surgery.Method: Publications up to November 2019 that compared the outcomes of LMH patients with and without LHEP who were managed by surgery were included. Forest plots were created using a weighted summary of proportion meta-analysis. A random effects mode was used and I2 heterogeneity estimates were calculated. Sensitivity analysis was carried out to evaluate the stability of the meta-analysis.Result: Four pertinent publications studying a total of 157 patients were identified. They were all retrospective studies and had a mean follow-up of at least 6 months. In all studies, the best corrected visual acuity (BCVA) had no significant differences before surgery and improved in both groups. The rate of REZ were different among studies included. Meta-analytic pooling of the improved BCVA between with and without LHEP groups was 0.15 (95 % CI, 0.02 to 0.27; p=0.02), and the pooled data revealed an odd ratio of 5.79 (95% CI, 1.15 to 29.18; p = 0.03) in the rate of restored ellipsoid zone, comparing the group without LHEP and the group with LHEP.Conclusion: Patients with LHEP were more likely to gain restored ellipsoid zone after surgery but had lower postoperative BCVA. The surgery of LMH with LHEP appeared to be useless for improving visual acuity.
Herpes Simplex Virus type 1 among people living with HIV in Mbeya, Tanzania
Authors: Mwakyula Issakwisa Habakkuk , Mbwile Gloria Reginald , Nassoro David Daniel , Mbwanji Godlove Fred , Ntinginya Nyanda Elias , Nsojo Anthony Ambikile
Published: December 2019

Background Clinical manifestations of HSV-1 infection is quite common in patients with HIV/AIDS. In Tanzania, nearly five percent of individuals aged 15 to 49 years are living with HIV, with the highest prevalence observed in the southern highland region. Studies are now reporting a growing HIV epidemic due to genital HSV-1. Despite such evidence, the prevalence of HSV-1 infection in HIV positive and negative individuals in the general Tanzanian population is mostly unknown. The objective of this study was to describe the prevalence of Herpes Simplex Virus type 1 infections among HIV positive and negative individuals in the Mbeya region, the third most affected region with HIV in Tanzania. Methods We conducted a study in the Mbeya region in the archived blood sera of participants from the recently completed EU funded EMINI project. A specially designed questionnaire was used to isolate the social and demographic characteristics of the study participants in the database. We tested archived participants’ sera for Herpes Simplex Virus type 1 using Virotech HSV-1 (gG1) ELISA (Enzygnost, Behring, Germany). Univariate and multivariate Poisson regression models were used to identify factors associated with HSV-1. Results A total of 640 adults were randomly recruited after stratification by HIV status, age, and sex. The overall seroprevalence of HSV-1 in the study population was 92.1%. The extrapolated prevalence estimate of Herpes Simplex Virus type 1 in the general population was 95.0% (96.0% in males versus 94.0% in females). Men and females were equally affected by HSV-1. HSV-1 was less prevalent in HIV positive individuals and was more common in individuals with elevated blood pressure. Conclusion People living with HIV were less likely to be HSV-1 positive. Further studies can evaluate whether the Herpes Simplex Virus type-1 can reduce the incidence of HIV infection.
Observation with or without late radiotherapy is equivalent to early radiotherapy in high-risk prostate cancer after radical prostatectomy: A SEER-Medicare analysis on trends, survival outcomes and complications
Authors: Kwon Young Suk Suk , Wang Wei , Srivast Arnav , Jang Thomas L , Eric Singer A , Parikh Rahul R , Kim Wun-Jae , Kim Isaac Yi
Published: December 2019

Introduction: While early radiotherapy (eRT) after radical prostatectomy (RP) has shown to improve oncologic outcomes in patients with high-risk prostate cancer (PCa) in a recent clinical trial, controversy remains regarding its benefit. We aimed to illustrate national trends of post-RP radiotherapy and compare outcomes and toxicities in patients receiving eRT vs. observation with or without late radiotherapy (lRT). Methods: Utilizing the Surveillance, Epidemiology and End Results (SEER)-Medicare data from 2001 to 2011, we identified 7557 patients with high-risk pathologic features after RP (≥ pT3N0 and/or positive surgical margins). Our study cohort was consisted of patients receiving RT within 6 months of surgery (eRT), those receiving RT after 6 months (IRT), and those never receiving RT (observation). Another subcohort, delayed RT (dRT), encompassed both IRT and observation. Trends of post-RP radiotherapy were compared using the Cochran-Armitage trend test. Cox regression models identified factors predictive of worse survival outcomes. Kaplan-Meier analyses compared the eRT and the dRT groups. Results: Among those with pathologically confirmed high-risk PCa after RP, 12.7% (n=959), 13.2% (n=1710), and 74.1% (n=4888) underwent eRT, lRT, and observation without RT, respectively. Of these strategies, the proportion of men on observation without RT increased significantly over time (p=0.004). Multivariable Cox regression model demonstrated similar outcomes between the eRT and the dRT groups. At a median follow up of 5.9 years, five-year overall and cancer-specific survival outcomes were more favorable in the dRT group, when compared to the eRT group. Radiation related toxicities, including urinary incontinence, erectile dysfunction, and urethral stricture, were higher in the eRT group when compared to the lRT group. Conclusions: Our results suggest that a blanket adoption of the eRT in high-risk PCa based on clinical trials with limited follow up may result in overtreatment of a significant number of men and expose them to unnecessary radiation toxicity.
Risk factors of initial inappropriate antibiotic therapy and the impacts on outcomes of neonates with gram-negative bacteremia
Authors: Chu Shih-Ming , Hsu Jen-Fu , Lai Mei-Yin , Huang Hsuan-Rong , Chiang Ming-Chou , Fu Ren-Huei , Tsai Ming-Horng
Published: December 2019

Background Timely appropriate empirical antibiotic plays an important role in critically ill patients with gram-negative bacteremia. However, the relevant data and significant impacts have not been well studied in the neonatal intensive care unit (NICU).Methods An 8-year (1 January 2007-31 December 2014) cohort study of all NICU patients with gram-negative bacteremia in a tertiary-care medical center was performed. Inadequate empirical antibiotic therapy was defined when a patient didn’t receive any antimicrobial agent to which the causative microorganisms were susceptible within 24 hour of blood culture sampling.Results Among 376 episodes of Gram-negative bacteremia, 75 (19.9%) received inadequate empirical antibiotic therapy. The cause of inadequate treatment was mostly due to the pathogen resistant to prescribed antibiotics (88.0%), and Pseudomonas aeruginosa (Odds ratio [OR]: 20.8, P < 0.001) and ESBL-producing bacteria (OR: 18.4, P < 0.001) had the highest risk. Previous exposure with 3rd generation cephalosporin was identified as the only independent risk factor (OR: 2.52, 95% CI: 1.18-5.37, P = 0.018). Empirically inadequately treated bacteremias were significantly more likely to have worse outcomes than those with adequate therapy, including more prolonged illness, higher rate of infectious complications (25.3% versus 9.3%, P < 0.001) and overall mortality (22.7% versus 11.0%, P = 0.013).Conclusions Inadequate empirical antibiotic therapy occurs in one-fifth of Gram-negative bacteremias in the NICU, and is associated with worse outcomes. Further effort to decrease emergence of antibiotic resistance and highly suspicion of infection by drug-resistant bacteria clinically is important to reduce rates of inadequacy.
The effect of improved housing on indoor mosquito density and exposure to malaria in the rural community of Minkoameyos, Centre Region of Cameroon.
Authors: Nguela Rachel Laure , Bigoga Jude D. , Armel Tedjou N. , Esther Tallah Tayie Ngobiyo , Dongmo Line Leukou , Boris Njeambosay A. , Frederic Tchouine , Kazi Riksum , Williams Peter , Mbacham Wilfred F. , Leke Rose G. F.
Published: December 2019

Background: Malaria control faces several threats. Alternative strategies to complement Long Lasting Insecticide-treated Nets and antimalarial therapy are therefore mandatory. This study evaluated the effectiveness of improved housing on indoor residual mosquito density and exposure to malaria-carrying Anophelines in Minkoameyos, a rural community in the center region of Cameroon.Methods: Following the identification of housing factors affecting malaria prevalence in 2013, 218 houses were improved (screening of doors and windows, installing plywood ceilings on open eaves, closing holes on the walls and doors). Quarterly surveys were conducted in a sample of 21 improved and 21 non-improved houses from November 2014 to October 2015. Mosquitoes sampled by night collections on human volunteers were identified morphologically. Their parity status determined. Mosquito infectivity was verified through Plasmodium falciparum CSP ELISA. The average entomological inoculation rates were determined. A Reduction Factor (RF), defined as the ratio of the values for mosquitoes collected outdoor to those collected indoor was calculated in improved houses (RFI) and non-improved houses (RFN). An Intervention Effect (IE=RFI/RFU) measured the true effect of the intervention. Chi-square test was used to determine variable significance. The threshold for statistical significance was set at P < 0.05.Results: A total of 1113 mosquitoes were collected comprising: Anopheles (58.6%), Culex (36.4%), Aedes (2.5%), Mansonia (2.4%) and Coquillettidia (0.2%). Amongst the anophelines were An. gambiae s.l. (95.2%), An. funestus (2.9%), An. ziemanni (0.2%), An. brohieri (1.2%) and An. paludis (0.5%). An gambiae s.s. was the only Anopheles gambiae sibling found. The intervention reduced the indoor Anopheles density by 1.8 fold (RFI=3.99; RFN=2.21; P=0.001 ). The indoor density of parous Anopheles was reduced by 1.7 fold (RFI=3.99; RFN=2.21; P=0.04 ) and that of infected Anopheles by 1.8 fold (RFI=3.26; RFN=1.78; P=0.04 ). Indoor peak biting rates were observed between 02am to 04am in non improved houses and from 02am to 06am in improved houses.Conclusion: Housing improvement reduced indoor residual anopheline density and malaria transmission. This highlights the need for Standardization and promotion of similar interventions to prevent malaria in comparable contexts.