bluuebunny/crossref_metadata_2025 huggingface dataset:
General Relativistic Magnetohydrodynamics Mean-Field Dynamos
Authors: Del Zanna  Luca , Tomei Niccolò , Franceschetti Kevin , Bugli Matteo , Bucciantini Niccolò
Published: February 2022

Large-scale, ordered magnetic fields in several astrophysical sources are supposed to be originated, and maintained against dissipation, by the combined amplifying action of rotation and small-scale turbulence. For instance, in the solar interior, the so-called α−Ω mean-field dynamo is known to be responsible for the observed 22-years magnetic cycle. Similar mechanisms could operate in more extreme environments, like proto neutron stars and accretion disks around black holes, for which the physical modelling needs to be translated from the regime of magnetohydrodynamics (MHD) and Newtonian gravity to that of a plasma in a general relativistic curved spacetime (GRMHD). Here we review the theory behind the mean field dynamo in GRMHD, the strategies for the implementation of the relevant equations in numerical conservative schemes, and we show the most important applications to the mentioned astrophysical compact objects obtained by our group in Florence. We also present novel results, such as three-dimensional GRMHD simulations of accretion disks with dynamo and the application of our dynamo model to a super massive neutron star, remnant of a binary neutron star merger as obtained from full numerical relativity simulations.
The Effect of Processing Methods on Food Quality and Human Health: Latest Advances and Prospects
Authors: Houška Milan , Silva Filipa Vinagre Marques
Published: February 2022

This Special Issue is focused on the use of modern food processing technologies to retain the highest possible content of health-promoting compounds in raw foods [...]
Effect of Lysosomal Cathepsin L on Proteolysis of Beef Myofibrillar Proteins In Vivo and In Vitro
Authors: Cui Baowei , Guo Xiuyun , Zhang Yawei , Meng Xiangren
Published: February 2022

This study investigated the effects of cathepsin L on proteolysis of beef myofibrillar proteins in vivo and in vitro. Results indicated that cathepsin L affected the degradation of desmin and troponin-T during postmortem aging, and the extent of degradation increased from 1 d to 14 d postmortem. No detectable degradation of titin, nebulin, and α-actinin in the presence of cathepsin L inhibitor was observed during postmortem aging. In vitro, cathepsin L affected the degradation of titin, nebulin, and troponin-T, and the extent of degradation increased with increasing incubation time. Nevertheless, cathepsin L did not cause the degradation of α-actinin and desmin, regardless of incubation temperature. The different results between in vitro and in vivo experiments might mainly depend on different treatment temperatures. Overall, these results indicated that cathepsin L participated in the degradation of myofibrillar proteins and meat tenderization.
Evolution in Composition of Kombucha Consortia over Three Consecutive Years in Production Context
Authors: Mas Perrine , Tran Thierry , Verdier François , Martin Antoine , Alexandre Hervé , Grandvalet Cosette , Tourdot-Maréchal Raphaëlle
Published: February 2022

Kombucha is a traditional drink obtained from sugared tea that is transformed by a community of yeasts and bacteria. Its production has become industrialized, and the study of the microbial community’s evolution is needed to improve control over the process. This study followed the microbial composition of black and green kombucha tea over three consecutive years in a production facility using a culture-dependent method. Microorganisms were isolated and cultivated using selective agar media. The DNA of isolates was extracted, amplified using 26S and 16S PCR, and sequenced. Identities were obtained after a comparison to the NCBI database. Dekkera/Brettanomyces bruxellensis, Hanseniaspora valbyensis and Saccharomyces cerevisiae were the major yeast species, and the major bacterial genera were Acetobacter and Liquorilactobacillus. Results highlight the persistence of yeast species such as B. bruxellensis detected in 2019. Some yeasts species appeared to be sensitive towards stressful events, such as a hot period in 2019. However, they were resilient and isolated again in 2021, as was the case for H. valbyensis. Dominance of B. bruxellensis was clear in green and black tea kombucha, but proportions in yeasts varied depending on tea type and phase (liquid or biofilm). Composition in acetic acid and lactic acid bacteria showed a higher variability than yeasts with many changes in species over time.
Tyrosinase Inhibitory and Antioxidant Activity of Enzymatic Protein Hydrolysate from Jellyfish (Lobonema smithii)
Authors: Upata Maytamart , Siriwoharn Thanyaporn , Makkhun Sakunkhun , Yarnpakdee Suthasinee , Regenstein Joe M. , Wangtueai Sutee
Published: February 2022

The optimization of antioxidant and anti-tyrosinase activity during jellyfish hydrolysate preparation was studied using a response surface methodology (RSM) with a face-centered composite design. The influence of the hydrolysis duration and the enzyme concentration on the IC50 of the DPPH and ABTS radical scavenging activity, ferric-reducing antioxidant power (FRAP), the degree of hydrolysis (DH), yield, and the IC50 value of tyrosinase inhibitory activity were determined. The optimum conditions for the production of jellyfish hydrolysate using alcalase (JFAH), flavourzyme (JFFH), or papain (JFPH) were achieved at hydrolysis times of 360, 345, or 360 min, respectively, and at an enzyme concentration of 5.0%. JFFH had the highest antioxidant and tyrosinase inhibitory activity. JFAH, JFFH, and JFPH concentrations of 2.5 mg/mL resulted in HaCaT cells (IC80) having a survival rate of 80%. The amino acid profile of JFFH contained about 43% hydrophobic and 57% hydrophilic amino acids, comprising Gly, Cys, Glx, Asx, which were dominant. The isolation of a peptide fraction from JFFH was carried out using ultrafiltration membranes (10, 3, and 1 kDa) and gel filtration chromatography. Fraction-III (1–3 kDa) showed the highest antioxidative and tyrosinase inhibitory activity.
Application of Micro- and Nano-Bubbles as a Tool to Improve the Rheological and Microstructural Properties of Formulated Greek-Style Yogurts
Authors: Babu Karthik Sajith , Liu Dylan Zhe , Amamcharla Jayendra K.
Published: February 2022

The objective of this study was to develop an alternative novel process technology for enhancing the rheological and functional properties of Greek-style yogurt (GSY). The GSY was formulated and prepared in the lab using micellar casein concentrate as a source of protein to achieve a protein content of 10% (w/w). The changes in physicochemical, microstructural, rheological, and functional properties of control (C-GSY) and micro- and nano-bubbles-treated GSY (MNB-GSY) were studied and compared before and after storage for 1, 2, 3, and 4 weeks. Before storage, the apparent viscosity at 100 s−1 (η100) was 1.09 Pa·s for C-GSY and 0.71 Pa·s for MNB-GSY. Incorporation of MNBs into GSY significantly (p < 0.05) decreased the η100 by 30% on 1 week of storage. Additionally, the η100 of MNB-GSY was lesser than C-GSY on week 2, 3, and 4 of storage. Notable microstructural changes and significant rheological differences were observed between the C-GSY and MNB-GSY samples. Differences were also noticed in syneresis, which was lower for the MNB-GSY compared with the control. Overall, the incorporation of MNBs into GSY showed considerable improvements in rheological and functional properties. Additionally, it’s a simple, cost-effective process to implement in existing GSY production plants.
Do Risky Scenarios Affect Forecasts of Savings and Expenses?
Authors: De Baets Shari , Önkal Dilek , Ahmed Wasim
Published: February 2022

Many people do not possess the necessary savings to deal with unexpected financial events. People’s biases play a significant role in their ability to forecast future financial shocks: they are typically overoptimistic, present-oriented, and generally underestimate future expenses. The purpose of this study is to investigate how varying risk information influences people’s financial awareness, in order to reduce the chance of a financial downfall. Specifically, we contribute to the literature by exploring the concept of ‘nudging’ and its value for behavioural changes in personal financial management. While of great practical importance, the role of nudging in behavioural financial forecasting research is scarce. Additionally, the study steers away from the standard default choice architecture nudge, and adds originality by focusing on eliciting implementation intentions and precommitment strategies as types of nudges. Our experimental scenarios examined how people change their financial projections in response to nudges in the form of new information on relevant risks. Participants were asked to forecast future expenses and future savings. They then received information on potential events identified as high-risk, low-risk or no-risk. We investigated whether they adjusted their predictions in response to various risk scenarios or not and how such potential adjustments were affected by the information given. Our findings suggest that the provision of risk information alters financial forecasting behaviour. Notably, we found an adjustment effect even in the no-risk category, suggesting that governments and institutions concerned with financial behaviour can increase financial awareness merely by increasing salience about possible financial risks. Another practical implication relates to splitting savings into different categories, and by using different wordings: A financial advisory institution can help people in their financial behaviour by focusing on ‘targets’, and by encouraging (nudging) people to make breakdown forecasts rather than general ones.
Spatial Modelling and Geovisualization of House Prices in the Greater Athens Region, Greece
Authors: Iliopoulou Polixeni , Feloni Elissavet
Published: February 2022

In this article, geovisualization is used for the presentation and interpretation of spatial analysis results concerning several house attributes. For that purpose, point data for houses in the region of Attica, Greece are analyzed. The data concern houses for sale and comprise structural characteristics, such as size, age and floor, as well as locational attributes. Geovisualization of house characteristics is performed employing spatial interpolation techniques, kriging techniques, in particular. Spatial autocorrelation in the data is examined through the calculation of the Moran’s I coefficient, while spatial clusters of houses with similar characteristics are identified using the Getis-Ord Gi* local spatial autocorrelation coefficient. Finally, a model is developed in order to predict house prices according to several structural and locational characteristics. In that respect, a classic hedonic pricing model is constructed, which is consequently developed as a geographically weighted regression (GWR) model in a GIS environment. The results of this model indicate that two characteristics, i.e., size and age, account for most of the variability in house prices in the study region. Since GWR is a local model producing different regression parameters for each observation, it is possible to obtain the spatial distribution of the regression parameters, which indicate the significance of the house characteristics for price determination in different locations in the study area.
Workshop on Blood Loss Quantification in Obstetrics: Improving Medical Student Learning through Clinical Simulation
Authors: Ruiz-Labarta Javier , Martínez Martín Ana , Pintado Recarte Pilar , González Garzón Blanca , Pina Moreno Juan Manuel , Sánchez Rodríguez Mercedes , Vallejo Gea África , Sordo Luis , Álvarez-Mon Melchor , Ortega Miguel A. , Arribas Coral Bravo , De León-Luis Juan A.
Published: February 2022

Purpose: To assess whether a clinical simulation-based obstetric blood loss quantification workshop for medical undergraduate trainees improves theoretical–practical knowledge, along with self-assurance and self-confidence. Methods: This was a quasi-experimental pre-post learning study conducted at the Gynaecology and Obstetrics Unit of the Hospital Gregorio Marañón, Madrid, Spain. Participants were volunteer students in their fourth year of a 6-year degree course in Medicine. The study period was divided into the stages: pre-workshop, intra-workshop, 2 weeks post-workshop and 6 months post-workshop. In the pre-workshop stage, students completed a brief online course in preparation for the workshop. The effectiveness of the workshop was assessed through multiple choice tests and self-administered questionnaires. Data were compared between time-points using statistical tests for paired samples. Results: Of the 142 students invited (age 21.94 ± 3.12 years), 138 accepted the offer of the workshop (97.2%), and 85.4% had no experience in managing blood loss. Between the stages pre- and 2 weeks post-workshop, significant improvements were observed in theoretical–practical knowledge (μ = 1.109), self-assurance and self-confidence. At the 6 months post-workshop stage, theoretical–practical knowledge diminished compared with 2 weeks post-workshop, returning to pre-workshop levels, while self-assurance and confidence failed to vary significantly in the longer term. Conclusions: The obstetric workshop improved theoretical–practical knowledge and the self-assurance and confidence of the medical students. Results 2 weeks post-workshop were maintained up until 6 months after the training intervention. The clinical simulation-based workshop was perceived by the students as useful and necessary.
Use of Physiotherapy Prior to Total Knee Arthroplasty—Results of the Prospective FInGK Study
Authors: Jacobs Hannes , Hoffmann Falk , Lazovic Djordje , Maus Uwe , Seeber Gesine H.
Published: February 2022

Background: Data regarding physiotherapy (PT) utilization prior to total knee arthroplasty (TKA) are insufficient. Therefore, this study aims to examine which percentage of patients receive PT within 12 months prior to TKA and which factors are associated with its use. Methods: Consecutive patients (≥18 years) undergoing primary or revision TKA in a German university hospital were recruited. A questionnaire including information on PT utilization, demography, and socioeconomics was collected one day prior to surgery and linked to medical hospital records. Multivariable logistic regression was conducted to determine variables associated with the use of PT. Results: A total of 241 out of 283 (85%) patients participated (60% female; mean age: 68.4 years). Overall, 41% received PT at least once during 12 months prior to TKA, women more frequently than men (48% vs. 29%). Although high disease burden was associated with increased utilization, about one in two in this condition did not receive PT. Multivariable logistic regression showed that age 75+ years, low education level, and moderate-to-severe depressive symptoms were associated with decreased PT utilization. Conclusions: We found low use of recommended PT management in patients prior to TKA. This potential underuse was even higher in some vulnerable subgroups, indicating inequalities. Prescribers as well as patients should integrate PT more consistently into osteoarthritis management.
Epigenetic Modifications in Lymphoma and Their Role in the Classification of Lymphomas
Authors: Harrop Sean , Yannakou Costas Kleanthes , Van Der Weyden Carrie , Prince Henry Miles
Published: February 2022

The characterisation of the lymphoma epigenome has provided insight into mechanisms involved in lymphomagenesis. Multiple lymphoma subtypes demonstrate recurrent mutations in key epigenetic regulators that have been utilised to define clinicogenetic groups that can predict clinical behaviour in these heterogenous entities. The high frequency of mutations in epigenetic regulators provides rationale to incorporate these in the classification of some subtypes of lymphoma. In addition, their recurrent nature provides a rationale to target such mutations, or the relevant pathway, for treatment. In this review, we summarised the available literature on epigenetic dysregulation in lymphoma and how it has been utilised in diagnosis and classification.
The Impact of Clay Minerals on the Building Technology of Vernacular Earthen Architecture in Eastern Austria
Authors: Feiglstorfer Hubert , Ottner Franz
Published: February 2022

The vernacular architecture in many regions in Eastern Austria was characterized by the use of unfired clay, at least until the 19th century, and in some areas until the 20th century. Farmhouses and associated farm buildings, such as storage buildings or press houses for the production of wine and cider, were erected using different earth construction techniques. The study area stretches from the Weinviertel, a region located in the province of Lower Austria in the north-east of Austria, to the Burgenland, a region located in the south-east of Austria, which belonged to Western Hungary until 1921. From a geological point of view, in the east of Austria—in the Vienna Basin and the Molasse Zone—huge areas of Tertiary clay are covered with loess deposits, which is the best-known basic material used in local earth-building traditions. A core question in the research on vernacular earthen heritage focuses on the impact of the geological conditions in Eastern Austria on the local earth-building techniques. The mineralogical composition of the different clays had an impact on the local building techniques. From a material-culture point of view, research on the relationship between the mineralogical properties of clay resources and local building techniques sheds light on the factors which influenced the evolution of certain vernacular building features. Tertiary clays and loess from the Pleistocene favoured the making of earth lumps, cob walls and adobe bricks over the whole Eastern Austrian region. Contrarily, regions in Burgenland with a high amount of gravel preferred, by tradition, to make walls by ramming. The clay mineral smectite acts as a binding agent in earth-building techniques over the whole investigated region—Weinviertel, Burgenland and Western Hungary.
Graded Moisture Deficit Effect on Secondary Metabolites, Antioxidant, and Inhibitory Enzyme Activities in Leaf Extracts of Rosa damascena Mill. var. trigentipetala
Authors: Hessini Kamel , Wasli Hanen , Al-Yasi Hatim M. , Ali Esmat F. , Issa Ahmed A. , Hassan Fahmy A. S. , Siddique Kadambot H. M.
Published: February 2022

Drought affects plant growth and yield in many agricultural areas worldwide by producing negative water potentials in the root zone that reduce water availability, affecting plant development and metabolism. This study investigated the effect of varying moisture regimes (100% field capacity (FC), well-watered plants, 50% FC (moderate water stress), and 25% FC (severe water stress)) on growth parameters, chlorophyll content, and bioactive molecule patterns, and the impact on antioxidant, lipoxygenase (LOX), and acetylcholinesterase (AChE) activities in Rosa damascena. The water deficit treatments reduced biomass production for both treatments (−29 and −33%, respectively, for MWS and SWS) and total chlorophyll (−18 and −38% respectively for MWS and SWS), relative to the control. The 50% FC treatment had the greatest effect on the phenolic profiles and their respective functionalities, with significant increases in the levels of total phenolic, benzoic (gallic, p-coumaric, and syringic acids) (+32%), and cinnamic (caffeic and trans-cinnamic acid) acids (+19%) and flavonoids (epicatechin-3-O-gallate) (+15%) compared to well-watered leaves (control leaves). The 50% FC treatment also exhibited the highest potential antioxidant activities (apart from NO-quenching activity), evidenced by the lowest IC50 and EC50 values. The inhibitory LOX and AChE capacities varied depending on the severity of stress, with superior activity in the 50% FC treatment. Overall, the drought tolerance in rose was associated mainly with its suitable manipulation of antioxidant production and orderly regulation of LOX and AChE activities.
Ab Initio Insight into the Interaction of Metal-Decorated Fluorinated Carbon Fullerenes with Anti-COVID Drugs
Authors: Katin Konstantin P. , Kochaev Alexey I. , Kaya Savas , El-Hajjaji Fadoua , Maslov Mikhail M.
Published: February 2022

We theoretically investigated the adsorption of two common anti-COVID drugs, favipiravir and chloroquine, on fluorinated C60 fullerene, decorated with metal ions Cr3+, Fe2+, Fe3+, Ni2+. We focused on the effect of fluoridation on the interaction of fullerene with metal ions and drugs in an aqueous solution. We considered three model systems, C60, C60F2 and C60F48, and represented pristine, low-fluorinated and high-fluorinated fullerenes, respectively. Adsorption energies, deformation of fullerene and drug molecules, frontier molecular orbitals and vibrational spectra were investigated in detail. We found that different drugs and different ions interacted differently with fluorinated fullerenes. Cr3+ and Fe2+ ions lead to the defluorination of low-fluorinated fullerenes. Favipiravir also leads to their defluorination with the formation of HF molecules. Therefore, fluorinated fullerenes are not suitable for the delivery of favipiravir and similar drugs molecules. In contrast, we found that fluorine enhances the adsorption of Ni2+ and Fe3+ ions on fullerene and their activity to chloroquine. Ni2+-decorated fluorinated fullerenes were found to be stable and suitable carriers for the loading of chloroquine. Clear shifts of infrared, ultraviolet and visible spectra can provide control over the loading of chloroquine on Ni2+-doped fluorinated fullerenes.
Role of Mitochondrial Pathways in Cell Apoptosis during He-Patic Ischemia/Reperfusion Injury
Authors: Zhang Sen , Rao Sijing , Yang Meiwen , Ma Chen , Hong Fengfang , Yang Shulong
Published: February 2022

Hepatic ischemia-reperfusion injury is a major cause of post-operative hepatic dysfunction and liver failure after transplantation. Mitochondrial pathways can be either beneficial or detrimental to hepatic cell apoptosis during hepatic ischemia/reperfusion injury, depending on multiple factors. Hepatic ischemia/reperfusion injury may be induced by opened mitochondrial permeability transition pore, released apoptosis-related proteins, up-regulated B-cell lymphoma-2 gene family proteins, unbalanced mitochondrial dynamics, and endoplasmic reticulum stress, which are integral parts of mitochondrial pathways. In this review, we discuss the role of mitochondrial pathways in apoptosis that account for the most deleterious effect of hepatic ischemia/reperfusion injury.
Immunomodulatory and Anti-Inflammatory Properties of Selenium-Containing Agents: Their Role in the Regulation of Defense Mechanisms against COVID-19
Authors: Mal’tseva Valentina N. , Goltyaev Michael V. , Turovsky Egor A. , Varlamova Elena G.
Published: February 2022

The review presents the latest data on the role of selenium-containing agents in the regulation of diseases of the immune system. We mainly considered the contributions of selenium-containing compounds such as sodium selenite, methylseleninic acid, selenomethionine, and methylselenocysteine, as well as selenoproteins and selenium nanoparticles in the regulation of defense mechanisms against various viral infections, including coronavirus infection (COVID-19). A complete description of the available data for each of the above selenium compounds and the mechanisms underlying the regulation of immune processes with the active participation of these selenium agents, as well as their therapeutic and pharmacological potential, is presented. The main purpose of this review is to systematize the available information, supplemented by data obtained in our laboratory, on the important role of selenium compounds in all of these processes. In addition, the presented information makes it possible to understand the key differences in the mechanisms of action of these compounds, depending on their chemical and physical properties, which is important for obtaining a holistic picture and prospects for creating drugs based on them.
Serial Passaging of RAW 264.7 Cells Modulates Intracellular AGE Formation and Downregulates RANKL-Induced In Vitro Osteoclastogenesis
Authors: Lucy Tanzima Tarannum , Mamun-Or-Rashid A. N. M. , Yagi Masayuki , Yonei Yoshikazu
Published: February 2022

The passage number of cells refers to the number of subculturing processes that the cells have undergone. The effect of passage number on morphological and phenotypical characteristics of cells is of great importance. Advanced glycation end products have also been associated with cell functionality and characteristics. Murine monocyte RAW 264.7 cells differentiate into osteoclasts upon receptor activation caused by nuclear factor-kappa-Β ligand (RANKL) treatment. This study aims to identify the role of passage number on intracellular advanced glycation end products (AGEs) formation and osteoclastogenic differentiation of RAW 264.7 cells. Western blotting was performed to check intracellular AGE formation along with fluorometric analysis using a microplate reader. Tartrate-resistant acid phosphatase (TRAP) staining was performed to check osteoclastogenic differentiation, and qPCR was realized to check the responsible mRNA expression. Immunofluorescence was used to check the morphological changes. Intracellular AGE formation was increased with passaging, and the higher passage number inhibited multinucleated osteoclastogenic differentiation. Osteoclastogenic gene expression also showed a reducing trend in higher passages, along with a significant reduction in F-actin ring size and number. Lower passages should be used to avoid the effects of cell subculturing in in vitro osteoclastogenesis study using RAW 264.7 cells.
Beyond Seizure Control: Treating Comorbidities in Epilepsy via Targeting of the P2X7 Receptor
Authors: Gil Beatriz , Smith Jonathon , Tang Yong , Illes Peter , Engel Tobias
Published: February 2022

Epilepsy is one of the most common chronic diseases of the central nervous system (CNS). Treatment of epilepsy remains, however, a clinical challenge with over 30% of patients not responding to current pharmacological interventions. Complicating management of treatment, epilepsy comes with multiple comorbidities, thereby further reducing the quality of life of patients. Increasing evidence suggests purinergic signalling via extracellularly released ATP as shared pathological mechanisms across numerous brain diseases. Once released, ATP activates specific purinergic receptors, including the ionotropic P2X7 receptor (P2X7R). Among brain diseases, the P2X7R has attracted particular attention as a therapeutic target. The P2X7R is an important driver of inflammation, and its activation requires high levels of extracellular ATP to be reached under pathological conditions. Suggesting the therapeutic potential of drugs targeting the P2X7R for epilepsy, P2X7R expression increases following status epilepticus and during epilepsy, and P2X7R antagonism modulates seizure severity and epilepsy development. P2X7R antagonism has, however, also been shown to be effective in treating conditions most commonly associated with epilepsy such as psychiatric disorders and cognitive deficits, which suggests that P2X7R antagonisms may provide benefits beyond seizure control. This review summarizes the evidence suggesting drugs targeting the P2X7R as a novel treatment strategy for epilepsy with a particular focus of its potential impact on epilepsy-associated comorbidities.
Development and Validation of SNP and InDel Markers for Pod-Shattering Tolerance in Soybean
Authors: Seo Jeong-Hyun , Dhungana Sanjeev Kumar , Kang Beom-Kyu , Baek In-Youl , Sung Jung-Sook , Ko Jee-Yeon , Jung Chan-Sik , Kim Ki-Seung , Jun Tae-Hwan
Published: February 2022

Pod-shattering causes a significant yield loss in many soybean cultivars. Shattering-tolerant cultivars provide the most effective approach to minimizing this loss. We developed molecular markers for pod-shattering and validated them in soybeans with diverse genetic backgrounds. The genes Glyma.16g141200, Glyma.16g141500, and Glyma.16g076600, identified in our previous study by quantitative trait locus (QTL) mapping and whole-genome resequencing, were selected for marker development. The whole-genome resequencing of three parental lines (one shattering-tolerant and two shattering-susceptible) identified single nucleotide polymorphism (SNP) and/or insertion/deletion (InDel) regions within or near the selected genes. Two SNPs and one InDel were converted to Kompetitive Allele-Specific PCR (KASP) and InDel markers, respectively. The accuracy of the markers was examined in the two recombinant inbred line populations used for the QTL mapping, as well as the 120 varieties and elite lines, through allelic discrimination and phenotyping by the oven-drying method. Both types of markers successfully discriminated the pod shattering-tolerant and shattering-susceptible genotypes. The prediction accuracy, which was as high as 90.9% for the RILs and was 100% for the varieties and elite lines, also supported the accuracy and usefulness of these markers. Thus, the markers can be used effectively for genetic and genomic studies and the marker-assisted selection for pod-shattering tolerance in soybean.
Field and Laboratory Efficacy of Low-Impact Commercial Products in Preventing Olive Fruit Fly, Bactrocera oleae, Infestation
Authors: Daher Elissa , Cinosi Nicola , Chierici Elena , Rondoni Gabriele , Famiani Franco , Conti Eric
Published: February 2022

The olive fruit fly, Bactrocera oleae, is the key pest of olive trees in several areas of the world. Given the need for the development of sustainable control methods, preventive tools, based on the manipulation of pest behaviour, must be considered. Here, under field and laboratory conditions, we tested the efficacy of different products in preventing B. oleae infestation. A field trial was conducted, from July to November 2020, in an olive orchard located in Central Italy. A table olive variety was selected and sprayed with rock powder, propolis, the mixture of both, copper oxychloride, or water (control). All treatments, except propolis, caused a reduction of B. oleae oviposition in olives, compared to the control. The mixture allowed the strongest reduction of fly infestation throughout the season, suggesting a synergistic effect. Behavioural no-choice assays were conducted to better understand the effects of treatments on B. oleae females. Compared to the control, females showed a lower preference for the central area of an arena containing an olive twig bearing two olive fruits, fully developed, but still green, treated with rock powder, plus propolis mixture. For all treatments, B. oleae showed lower oviposition events, suggesting deterrence to oviposition. Our results indicate that the tested products may have value against B. oleae, within integrated pest management (IPM) and organic agriculture.
Peripheral Coronary Artery Circulatory Dysfunction in Remote Stage Kawasaki Disease Patients Detected by Adenosine Stress 13N-Ammonia Myocardial Perfusion Positron Emission Tomography
Authors: Tsuno Kanae , Fukazawa Ryuji , Kiriyama Tomonari , Imai Shogo , Watanabe Makoto , Kumita Shinichiro , Itoh Yasuhiko
Published: February 2022

Coronary peripheral circulatory disturbances in the remote stage of Kawasaki disease have been reported. In this study, of the 50 patients in the remote stage of Kawasaki disease who underwent coronary perfusion evaluation using adenosine-loaded 13N-ammonia positron emission tomography, 28 patients who did not have stenosis of ≥75% in the left coronary artery underwent an evaluation for myocardial flow reserve (MFR) of the left anterior descending artery (LAD) and left circumflex artery (LCx). Clinical findings were compared between patients with normal (≥2.0) and abnormal (<2.0) MFRs. In the group with an abnormal MFR in the LAD, the responsiveness of the coronary vascular resistance to adenosine stress decreased even in the LCx (3.50 ± 1.23 vs. 2.39 ± 0.25, p = 0.0100). In the group with an abnormal MFR in the LCx, the responsiveness of the coronary vascular resistance in the LAD also decreased (3.27 ± 1.39 vs. 2.03 ± 0.25, p = 0.0105), and the age of onset of Kawasaki disease tended to be younger in the group with abnormal MFR in the LAD and LCx. We found that the peripheral coronary circulation was extensively impaired in the remote stage of Kawasaki disease, suggesting that an early onset of Kawasaki disease may affect the peripheral coronary circulation in later years.
Against All Odds? Addiction History Associated with Better Viral Hepatitis Care: A Dutch Nationwide Claims Data Study
Authors: Von den Hoff Daan W. , Berden Floor A. C. , Atsma Femke , Schellekens Arnt F. A. , Drenth Joost P. H.
Published: February 2022

The elimination of viral hepatitis in target populations is crucial in reaching WHO viral hepatitis elimination goals. Several barriers for the treatment of viral hepatitis in people with addictive disorders have been identified, yet nationwide data on hepatitis healthcare utilization (HCU) in these patients are limited. We investigated whether a history of addictive disorder is associated with suboptimal hepatitis HCU, indicating failure to receive diagnostic care or treatment. We identified all newly referred viral hepatitis patients in the Netherlands between 2014 and 2019 by query of the Dutch national hospital claims database. Each patient’s first year of HBV or HCV care activities was collected and clustered in two categories, ‘optimal’ or ‘suboptimal’ hepatitis HCU. Optimal HCU includes antiviral therapy. We tested the association between addiction history and HCU, adjusted for sex, age, migrant status, and comorbidity. In secondary analyses, we explored additional factors affecting hepatitis HCU. We included 10,513 incident HBV and HCV patients, with 13% having an addiction history. Only 47% of all patients achieved optimal hepatitis HCU. Addiction history was associated with less suboptimal HCU (adjusted OR = 0.73, 95% CI = 0.64–0.82). Migration background was associated with suboptimal HCU (OR = 1.62, 95% CI = 1.50–1.76). This study shows that addiction history is associated with higher viral hepatitis HCU; thus, this population performs better compared to non-addicted patients. However, less than 50% of all patients received optimal hepatitis care. This study highlights the need to improve hepatitis HCU in all patients, with a focus on migrant populations. Linkage to care in the addicted patients is not studied here and may be a remaining obstacle to be studied and improved to reach WHO viral hepatitis elimination goals.
The Fight against Infectious Diseases: The Essential Role of Higher-Order Thinking and Problem-Solving
Authors: Gottschling Juliana , Krieger Florian , Greiff Samuel
Published: February 2022

The development of a vaccine marks a breakthrough in the fight against infectious diseases. However, to eradicate highly infectious diseases globally, the immunization of large parts of the population is needed. Otherwise, diseases, such as polio, measles, or more recently COVID-19, will repeatedly flare-up, with devastating effects on individuals and, in the worst case, on significant shares of the world population. For example, polio has been almost eradicated over the past three decades through an unprecedented global effort, but complete immunization has not yet been achieved. In this article, we use polio as an example to show how the global effort of developing and administering a vaccine can be understood as solving a complex problem since it involves cultural, political, and geographical barriers that demand solutions in dynamically changing and highly versatile environments. Referring to the literature on problem-solving, higher-order thinking, and complex reasoning, we demonstrate how the ability to deal with real-world environments that are complex and dynamically changing, adapting initial solutions to new circumstances and collaborate efficiently with others, has been essential for this endeavor. We argue that problem-solving abilities form one basis for solving consequential world problems.
Ship Traffic Flow Prediction in Wind Farms Water Area Based on Spatiotemporal Dependence
Authors: Xu Tian , Zhang Qingnian
Published: February 2022

To analyze the changing characteristics of ship traffic flow in wind farms water area, and to improve the accuracy of ship traffic flow prediction, a Gated Recurrent Unit (GRU) of a Recurrent Neural Network (RNN) was established to analyze multiple traffic flow sections in complex waters based on their traffic flow structure. Herein, we construct a spatiotemporal dependence feature matrix to predict ship traffic flow instead of the traditional ship traffic flow time series as the input of the neural network. The model was used to predict the ship traffic flow in the water area of wind farms in Yancheng city, Jiangsu Province. Autoregressive Integrated Moving Average (ARIMA), Support-Vector Machine (SVM) and Long Short-Term Memory (LSTM) were chosen as the control tests. The GRU method based on the spatiotemporal dependence is more accurate than the current mainstream ship traffic flow prediction methods. The results verify the reliability and validity of the GRU method.
Numerical Simulation Study of a Swirling Drill Bit Used for Ice Core Drilling
Authors: Wang Mengke , He Wenbo , Wang Minqi , Cao Jine , Cao Pinlu
Published: February 2022

Due to its high mechanical penetration rate and lack of pollution of the environment, air reverse circulation drilling is considered to be a promising method for ice drilling. The air reverse circulation is caused by the combination of the ejector and the flushing nozzles in the drill bit. In this paper, CFD software was used to simulate the influence of the structure of the swirler on the effect of air reverse circulation in the swirling drill bit, and a testing stand was established for the testing of air reverse circulation. The results show that for drill bits without flushing nozzles, the smaller the helical angle is, the larger the entrainment ratio will be, meanwhile the smaller the area ratio is, the larger the entrainment ratio will be. In contrast, for drill bits designed with flushing nozzles, the larger the helical angle is, the larger the entrainment ratio will be, and the larger the area ratio is, the larger the entrainment ratio will be. In addition, the presence of the ice core sharply reduces the effect of air reverse circulation. When the ice core’s height exceeds that of the outlet of the swirler, the reverse circulation effect is slightly improved.
Transcriptional Network in Colletotrichum gloeosporioides Mutants Lacking Msb2 or Msb2 and Sho1
Authors: Liu Na , Meng Fanli , Tian Chengming
Published: February 2022

Colletotrichum gloeosporioides is a hemibiotrophic ascomycetous fungus that causes anthracnose in many plants worldwide. During infections, C. gloeosporioides produces an appressorium in response to various plant surface signals. However, the mechanism mediating host surface signal recognition remains unclear. In this study, C. gloeosporioides ΔCgMsb2 and ΔCgMsb2Sho1 mutants lacking hypothetical sensors of plant surface signals were examined. The mutations in ΔCgMsb2 and ΔCgMsb2Sho1 adversely affected conidial size and sporulation, while also inhibiting growth. Significant transcriptional changes were detected for nearly 19% and 26% of the genes in ΔCgMsb2 and ΔCgMsb2Sho1, respectively. The lack of these plasma membrane receptors altered the expression of specific genes, especially those encoding hydrolases, ABC transporters, and mitogen-activated protein kinases (MAPKs). The encoded MAPKs participate in the signal transduction of ERK and JNK signaling pathways, activate downstream signals, and contribute to metabolic regulation. Our data demonstrate that the C. gloeosporioides membrane proteins Msb2 and Sho1 affect gene regulation, thereby influencing conidial growth, metabolism, and development. These findings provide new insights into the regulation of C. gloeosporioides’s development and infection of plant hosts.
Conventional Antifungals for Invasive Infections Delivered by Unconventional Methods; Aerosols, Irrigants, Directed Injections and Impregnated Cement
Authors: Drew Richard H. , Perfect John R.
Published: February 2022

The administration of approved antifungals via unapproved formulations or administration routes (such as aerosol, direct injection, irrigation, topical formulation and antifungal-impregnated orthopedic beads or cement) may be resorted to in an attempt to optimize drug exposure while minimizing toxicities and/or drug interactions associated with conventional (systemic) administrations. Existing data regarding such administrations are mostly restricted to uncontrolled case reports of patients with diseases refractory to conventional therapies. Attribution of efficacy and tolerability is most often problematic. This review updates prior published summaries, reflecting the most recent data and its application by available prevention and treatment guidelines for invasive fungal infections. Of the various dosage forms and antifungals, perhaps none is more widely reported than the application of amphotericin B-containing aerosols for the prevention of invasive mold infections (notably Aspergillus spp.).
The Bucharest Stock Exchange: A Starting Point in Structuring a Valuable CSR Index
Authors: Panait Mirela Clementina , Voica Marian Catalin , Hysa Eglantina , Siano Alfonso , Palazzo Maria
Published: February 2022

The aim of this article was to identify the role and specific mechanisms of the stock exchange in promoting corporate social responsibility (CSR) and CSR communications among companies listed on the Romanian capital market given country membership of the European Union. Taking into account the quality of the Bucharest Stock Exchange (BSE) as a member of the Sustainable Stock Exchanges, as well as BSE’s concerns about promoting CSR, a CSR index was built to capture the specific actions of companies listed on this market. The public companies were considered representative for the promotion of CSR based on their size and other relevant features. The index can be seen by companies that can further develop it, test its validity, and employ it as a tool to reassure investors who will decide to spend their money to buy shares and stocks of organizations ranked in the BSE.
A Model for Brucellosis Disease Incorporating Age of Infection and Waning Immunity
Authors: Kenne Cyrille , Mophou Gisèle , Dorville René , Zongo Pascal
Published: February 2022

This paper proposes a model for brucellosis transmission. The model takes into account the age of infection and waning immunity, that is, the progressive loss of immunity after recovery. Three routes of transmissions are considered: vertical transmission, and both direct and indirect routes of horizontal transmission. According to the well-posedness results, we provide explicit formulas for the equilibria. Next, we derive the basic reproduction number R0 and prove some stability results depending on the basic reproductive number. Finally, we perform numerical simulations using model parameters estimated from biological data to confirm our theoretical results. The results of these simulations suggest that for certain values of parameters, there will be periodic outbreaks of epidemics, and the disease will not be eradicated from the population. Our results also highlight the fact that the birth rate of cattle significantly influences the dynamics of the disease. The proposed model can be of a good use in studying the effects of vaccination on the cattle population.
Infrared Small Target Detection Based on Partial Sum Minimization and Total Variation
Authors: Rawat Sur Singh , Alghamdi Saleh , Kumar Gyanendra , Alotaibi Youseef , Khalaf Osamah Ibrahim , Verma Lal Pratap
Published: February 2022

In the advanced applications, based on infrared detection systems, the precise detection of small targets has become a tough work today. This becomes even more difficult when the background is highly dense in addition to the nature of small targets. The problem raised above is solved in various ways, including infrared patch image (IPI) based methods which are considered to have the best performance. In addition, the greater shrinkage of singular values in the methods based on IPI leads to the problem of nuclear norm minimization (NNM), which leads to the problem of incorrectly recognizing small targets in a highly complex background. Hence, this paper proposed a new method for infrared small target detection (ISTD) via total variation and partial sum minimization (TV-PSMSV). The proposed TV-PSMVS in this work basically replaces the IPI’s NNM with partial sum minimization (PSM) of singular values and, additionally, the total variance (TV) regularization term is inducted to the background patch image (BPI) to suppress the complex background and enhance the target object of interest. The mathematical solution of the proposed TV-PSMSV approach was performed using alternating direction multiplier (ADMM) to verify the proposed solution. The experimental evaluation using real and synthetic data set was performed, and the result revealed that the proposed TV-PSMSV outperformed existing referenced methods in the terms of background suppression factor (BSF) and the signal to gain ratio (SCRG).
Leukocytosis and C-Reactive Protein May Predict Development of Secondary Cerebral Vasospasm in Patients with Aneurysmal Subarachnoid Hemorrhage
Authors: Buce-Satoba Ieva , Rozkalne Daina , Mamaja Biruta , Krumina Gaida , Ozolina Agnese
Published: February 2022

Background and Objectives: Secondary cerebral vasospasm (CV) with subsequent delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH) remains an unpredictable pathology. The aim of this retrospective study was to investigate the association between inflammatory parameters, white blood cell (WBC) count, and C-reactive protein plasma levels (CRP) and the occurrence of secondary CV in patients with aSAH. Materials and Methods: The medical records of 201 Intensive Care Unit patients in Riga East University Hospital with aSAH were retrospectively reviewed in a 24-month period. WBC count and CRP values were observed at admission to the hospital and on the third day. According to the inclusion criteria, 117 (48 males) participants were enrolled for further analysis, with average age of 56 ± 15 years (mean ± SD). In total, secondary CV was diagnosed in 21.4% of cases, and DCI in 22.4% of cases. The patients were classified into three groups: SAH-CV group (n = 25), SAH-DCI group (n = 12), and SAH or control group (n = 80), for comparative analysis. Results: We found that SAH-CV patients demonstrated notably higher inflammatory parameters compared to controls: WBC 13.2 ± 3.3 × 109/L vs. 11.2 ± 3.7 × 109/L; p = 0.01 and CRP median 9.3 mg/L vs. 1.9 mg/L; p < 0.001, respectively. We found that the odds of developing CV increased by 5% for each CRP increase of 1 mg/L at admission (OR, 1.05; CI, 1.014–1.087; p = 0.006). Concomitantly, the odds increased by 16% for every rise in WBC count of 1 × 109/L (OR, 1.16; CI, 1.02–1.32; p = 0.02). WBC count was associated with the occurrence of CV with 96% sensitivity and 40% specificity, with a cut off level of 10.015 × 109/L and AUC 0.683; p = 0.006. CRP displayed 54% sensitivity and 90% specificity with a cut off value of 8.9 mg/L and AUC 0.751; p < 0.001. Moreover, higher values of inflammatory parameters at admission correlated with a longer stay in ICU (r = 0.3, p = 0.002 for WBC count and r = 0.305, p = 0.002 for CRP values), and poor outcome (death) was significantly associated with higher CRP values at admission and on the third day (16.1. vs. 2.2. and 57.4. vs. 11.1, p < 0.001, respectively). Higher mortality was detected in SAH-CV patients (32%) compared to controls (6.3%; p < 0.001). Conclusions: Inflammatory parameters such as WBC count and CRP values at admission might be helpful to predict the development of secondary CV.