bluuebunny/crossref_metadata_2025 huggingface dataset:
Lessons Learned from Four Real-Life Case Studies: Energy Balance Calculations for Implementing Positive Energy Districts
Authors: Bruckner Helmut , Alyokhina Svitlana , Schneider Simon , Binder Manuela , Abdin Zain Ul , Santbergen Rudi , Verkou Maarten , Zeman Miro , Isabella Olindo , Pagliarini Marco , Botta Cristiana , Streche Ana
Published: January 2025

Positive Energy Districts (PEDs) are integral to achieving sustainable urban development by enhancing energy self-sufficiency and reducing carbon emissions. This paper explores energy balance calculations in four diverse case study districts within different climatic conditions—Fiat Village in Settimo Torinese (Italy), Großschönau (Austria), Beursplain in Amsterdam (Netherlands), and Lunca Pomostului in Reşiţa (Romania)—as part of the SIMPLY Positive project. Each district faces unique challenges, such as outdated infrastructure or heritage protection, which we address through tailored strategies including building renovations and the integration of renewable energy systems. Additionally, we employ advanced simulation methodologies to assess energy performance. Simulation results highlight the significance of innovative technologies like photovoltaic-thermal (PVT) systems, application of demand-side actions, and flexible grid usage. Furthermore, mobility assessments and resident-driven initiatives demonstrate the critical role of community engagement in reducing carbon footprints. This study underscores the adaptability of PED frameworks across varied urban contexts and provides actionable insights for scaling similar strategies globally, supporting net-zero energy targets.
Single Nucleotide Polymorphism Markers Based on Hyper-Seq Revealed the Genetic Diversity and Genetic Relationship of Horsfieldia hainanensis in China
Authors: Xu Jianwang , Zou Rong , Chai Shengfeng , Jiang Haolong , Zhong Wentong , Tang Jianmin , Wei Xiao
Published: January 2025

Horsfieldia hainanensis is China’s second-class key protected wild plant with high scientific research, timber, and medicinal values. In this study, the SNP molecular marker technology was used to determine the genetic diversity, genetic structure, genetic differentiation, genetic relationship, and its influencing factors of H. hainanensis populations. It provided a scientific basis for the formulation of its protection strategy. The results showed that (1) the genetic diversity of H. hainanensis populations exhibited a very low level (π = 0.0011). (2) The overall genetic differentiation of H. hainanensis populations was relatively large. There was a significant genetic differentiation between Hainan populations and Guangxi and Yunnan populations on the mainland (FST = 0.556); the genetic differentiation between Guangxi and Yunnan populations was relatively smaller (FST = 0.242). There is a natural water barrier around Hainan Island that reduces the gene exchange between island populations and mainland populations. Therefore, geographical isolation is one of the main factors affecting the genetic differentiation of H. hainanensis populations (p = 0.0103 < 0.05). (3) The genetic relationship between Hainan populations and Yunnan and Guangxi populations was relatively distant, while the genetic relationship between Yunnan and Guangxi populations was relatively closer. In conclusion, it is recommended that during in situ protection, the Guangxi population with higher genetic diversity should be preferentially protected, especially the populations in NG and SL should be regarded as the key protected populations of wild populations. The gene exchange between Yunnan population (MLP) and Guangxi populations (NG, SL, DX1, and DX2) should be enhanced by means of artificial pollination in different localities. During ex situ protection, the germplasm resource collection work of the population (NG, SL) with higher genetic diversity should be carried out preferentially. The technical research on tissue culture and rapid propagation should be actively carried out. A germplasm resource nursery should be established. The individuals should be planted according to provenance and family, but the planting distance between individuals can be appropriately reduced to increase the gene exchange between individuals and make up for the defects during in situ protection.
Transcriptome Analysis of Magnolia sieboldii K. Koch in Response to Heat Stress
Authors: Wang Jinling , Wang Yaling , Wang Ruijian , Wang Jing , Kang Yongxiang
Published: January 2025

Magnolia sieboldii K. Koch is a relict plant species that survived in the glacial period. The species possesses significant esthetic value and is predominantly found in vertically stratified high-altitude forests located in southern China. The primary limiting factor for urban greening when introducing high-altitude species to low-altitude areas is excessive temperature. However, the response mechanism of M. sieboldii to elevated temperatures remains unclear. In this study, we employed the RNASeq technique to investigate the response mechanism of M. sieboldii under heat stress conditions. A total of 88,746 unigenes were obtained, with over 36.51% of these unigenes being annotated in at least one publicly available database. The comparison of the 35 °C and 40 °C treatment groups with the control group revealed a total of 7470 and 13,494 differentially expressed genes (DEGs), respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that the differentially up-regulated and down-regulated genes were implicated in plant–pathogen interactions, plant hormone signal transduction, and the MAPK signaling pathway-plant. Differential expression genes associated with the response to heat stress were also observed, including transcription factors such as AP2/EREBPs, WRKY, NACs, MYBs, bZIPs, and HSFs. These transcription factors may collectively modulate cellular metabolism, signal transduction pathways, and the synthesis as well as degradation of response proteins in M. sieboldii. In addition, network analysis using STRING on different genes revealed that the central node proteins in the network were CLPB1, HSP70-4, HOP3, P58IPK, HSP90-2, ERDJ3B, and MBF1C, all of which exhibited associations with heat tolerance. The findings of this study enhance our comprehension of the molecular regulatory mechanism underlying heat stress in M. sieboldii, which holds significant implications for investigating its translocation from high-altitude to low-altitude regions and ex situ conservation.
Soil Comprehensive Fertility Changes in Response to Stand Age and Initial Planting Density of Long-Term Spacing Trials of Chinese Fir Plantations
Authors: Sun He , Lei Jie , Liu Juanjuan , Zhang Xiongqing , Yuan Deyi , Duan Aiguo , Zhang Jianguo
Published: January 2025

The growing demand for wood products and ecosystem services in Chinese fir plantations has led to longer rotation ages and density control practices, raising concerns about their impact on soil fertility. This study assessed soil fertility of Chinese fir plantations in Fujian, Jiangxi, and Sichuan Provinces using the Nemerow index. The effects of stand age and initial planting density on soil fertility were analyzed using statistical models. In Fujian and Jiangxi, soil fertility was significantly higher at 11 and 30 years than at 5 and 25 years, while in Sichuan, it was higher at 25 and 30 years than at 5 and 11 years. In Fujian, soil fertility was higher at 6667 trees ha−2 than at 1667 trees ha−2. No significant differences were observed in Jiangxi, while in Sichuan, soil fertility at 6667 trees ha−2 was significantly higher than at 5000 and 1667 trees ha−2, and soil fertility at 10,000 trees ha−2 exceeded that at 1667 trees ha−2. Soil fertility typically increased with stand age, especially in Fujian and Sichuan. Soil fertility also increased with initial planting density, especially in Jiangxi and Sichuan. A structural equation model (SEM) explained 88% of the variance in soil fertility, with stand age directly affecting soil fertility and soil organic matter mediating the effects of stand age and planting density. These findings suggest that adjusting rotation age and planting density could help improve soil fertility, offering practical implications for sustainable forest management in Chinese fir plantations.
Long-Term Growth Trends of 18 Native and Non-Native Tree Species Based on Data from Experimental Plots Since 1878 in Brandenburg, Germany
Authors: Engel Markus , Hagemann Ulrike , Schröder Jens
Published: January 2025

The rapid environmental changes associated with climate change increase the need for adaptation strategies in forest management based on profound knowledge about tree species, particularly in the context of assisted migration. For research purposes, selected native and non-native tree species were planted in Brandenburg, Germany more than 120 years ago. Today, these sites provide an opportunity to gather insights about their performance and growth-response throughout the past century. We analyzed the height growth increment of 18 tree species on 1765 long-term experimental plots, the earliest of which have been monitored since 1878. We additionally investigated the stand-level volume increments on 60 unmanaged plots for two of these species. Our results show increasing trends in forest stand growth for Scots pine (Pinus sylvestris L.) and Sessile oak (Quercus petraea (Matt.) Liebl.). However, long-term height increment showed positive, negative, and indifferent growth trends in reaction to changing environmental conditions. Remarkably, 16 out of 18 species showed a growth decline between the years 2000 and 2020, likely attributable to increasing frequencies of single and consecutive drought events. We found non-native species to perform comparably to native tree species. Forest management should reconsider the role of native and non-native species in climate-adapted forests. We recommend focusing on provenance and local site adaptability in assisted migration efforts and argue that maintenance of long-term experiments can provide us with valuable insights on species performance in the near future.
Effect of GFRP and CFPR Hybrid Confinement on the Compressive Performance of Concrete
Authors: Moretti Marina L.
Published: January 2025

Application of hybrid jackets consisting of comparatively stiff FRP materials for the seismic retrofit of substandard RC columns, aiming at reducing the risk of buckling and of brittle failure, which are typical to older columns, is a promising challenge. Given the sparsity of similar experimental data, the objective of this paper is to study the hybrid effect in concrete confined with conventional carbon- and glass- reinforced polymer fabrics (CFRP and GFRP, respectively). Twenty-six concrete cylinders, wrapped by one to three layers of CFRP and GFRP with different fiber configurations, were tested in compression. A clear hybrid effect was observed, consisting of a less brittle failure and an improved confinement as compared to the behavior of simple jackets. Furthermore, hybrid specimens, in which a CFRP layer is substituted by a GFRP layer, appear to display similar efficiency in confinement compared to specimens with a stiffer jacket consisting of more CFRP sheets, which are expected to experience 30 to 40% higher lateral pressure owing to the stiffer jacket. A design model to estimate peak concrete compressive strength and axial strain is proposed. The results are promising towards the potential application of similar hybrid jackets for the seismic rehabilitation of older RC columns.
Improving Combustion Analysis of Extruded Polystyrene via Custom Isolation Methodology
Authors: Hou Yanan , Wan Mei , Li Jian , Ren Fei , Qian Xiaodong , Shi Congling
Published: January 2025

This study is dedicated to an in−depth analysis of the combustion characteristics of extruded polystyrene (XPS) as a building insulation material with the aim of accurately assessing its fire risk in the built environment. Innovatively, this research employed a cone calorimeter equipped with a self−designed insulating sample holder to conduct a systematic experimental study. Additionally, it performed a comprehensive analysis of the ignition characteristics, heat release rate, fire hazard, smoke release, and toxic gas emission of XPS materials. The experimental results revealed that the combustion behavior of XPS is influenced by multiple factors, including the content of flame retardants and external heat flux, which significantly affect the fire hazard of XPS. When the thermal radiation intensity escalates from 25 kW/m2 to 55 kW/m2, the peak heat release rate of XPS−B1 rises from 428 kW/m2 to 535 kW/m2, marking an increase of 25.00%. Conversely, the peak heat release rate of XPS−B2 surges from 348 kW/m2 to 579 kW/m2, reflecting a substantial increase of 66.38%. This research not only provides a solid theoretical foundation and detailed experimental data for the fire behavior of XPS materials but also holds significant practical importance for enhancing the fire safety of buildings. Overall, this research contributes to the scientific understanding of XPS insulation materials and supports the development of more effective fire prevention measures in construction.
Fish Community Diversity and Spatiotemporal Dynamics in the Downstream of the Fujiang River Based on Environmental DNA
Authors: Zhang Jiaming , Chen Yifang , Zhou Xinxin , Huang Jiaxin , Dong Xiaohan , Zhu Shuli , Shen Yanjun
Published: January 2025

Hydrological changes caused by dam construction are among the primary drivers of global freshwater biodiversity decline. To assess the current status of fish community diversity and examine the impacts of cascade hydropower development on fish diversity, this study employed environmental DNA (eDNA) technology from 2023 to 2024 to conduct seasonal surveys at 18 sampling sites across six river segments separated by five dams in the downstream section of the Fujiang River. The study aimed to uncover the temporal and spatial dynamics of fish diversity and community structure, as well as to analyze the influence of environmental factors on these patterns. The results identified 84 fish species spanning 60 genera, 19 families, and 7 orders, including 2 nationally protected species, 11 endemic species of the upper Yangtze River, and 13 alien species. The cascade dams were found to have significantly reduced fish diversity compared to historical records, with a marked decline in native species and a rise in alien species, contributing to the miniaturization and homogenization of fish communities. Environmental factor analysis revealed that chemical oxygen demand (COD), dissolved oxygen (DO), total dissolved solids (TDS), electrical conductivity (EC), and reservoir formation time were significant drivers of fish community structure and diversity. This study provides essential baseline data on fish diversity under the influence of cascade hydropower development in the Fujiang River. It also offers valuable insights into the current status of fish resources and supports efforts in fish conservation and aquatic ecosystem management in the region.
Phytochemicals, Organic Acid, and Vitamins in Red Rhapsody Strawberry—Content and Storage Stability
Authors: Hong Hung Trieu , Rami Julius , Rychlik Michael , O’Hare Tim J. , Netzel Michael E.
Published: January 2025

Strawberries are highly perishable fruits harvested at full ripeness, and their nutritional quality together with their phytochemical composition can be significantly affected by storage duration and temperature. This study investigated the changes in key bioactive compounds, including folate, vitamin C, anthocyanins, quercetin-3-glucoside, ellagic acid, and organic acids, in “Red Rhapsody” strawberries stored at two typical household temperatures (4 °C and 23 °C). While storage duration and temperature did not have a significant impact (p > 0.05) on folate content, significant changes in other phytochemicals were observed. The total anthocyanin content increased significantly (p < 0.05), from 30.0 mg/100 g fresh weight (FW) at Day 0 to 84.4 mg/100 g FW at Day 7 at 23 °C, a 2.8-fold increase. Conversely, the vitamin C content was significantly reduced (p < 0.05), from 54.1 mg/100 g FW at Day 0 to 28.4 mg/100 g FW at Day 7 at 23 °C, while it remained stable at 4 °C. Additionally, the concentrations of quercetin-3-glucoside, ellagic acid, and organic acids underwent significant changes during the storage period. The total folate content fluctuated between 73.2 and 81.6 μg/100 g FW at both temperatures. These results suggest that storage temperature and duration influence the individual phytochemicals and nutrients of strawberries differently, with potential implications for their nutritional value and bioactive compound content.
Advances in Computer Vision and Spectroscopy Techniques for Non-Destructive Quality Assessment of Citrus Fruits: A Comprehensive Review
Authors: Yu Kai , Zhong Mingming , Zhu Wenjing , Rashid Arif , Han Rongwei , Virk Muhammad Safiullah , Duan Kaiwen , Zhao Yongjun , Ren Xiaofeng
Published: January 2025

Citrus fruits, classified under the Rutaceae family and Citrus genus, are valued for their high nutritional content, attributed to their rich array of natural bioactive compounds. To ensure both quality and nutritional value, precise non-destructive testing methods are crucial. Among these, computer vision and spectroscopy technologies have emerged as key tools. This review examines the principles and applications of computer vision technologies—including traditional computer vision, hyperspectral, and multispectral imaging—as well as various spectroscopy techniques, such as infrared, Raman, fluorescence, terahertz, and nuclear magnetic resonance spectroscopy. Additionally, data fusion methods that integrate these technologies are discussed. The review explores innovative uses of these approaches in Citrus quality inspection and grading, damage detection, adulteration identification, and traceability assessment. Each technology offers distinct characteristics and advantages tailored to the specific testing requirements in Citrus production. Through data fusion, these technologies can be synergistically combined, enhancing the accuracy and depth of Citrus quality assessments. Future advancements in this field will likely focus on optimizing data fusion algorithms, selecting effective preprocessing and feature extraction techniques, and developing portable, on-site detection devices. These innovations will drive the Citrus industry toward increased intelligence and precision in quality control.
Enhancing Rice Bran Soluble Dietary Fiber Yield Through Sequential Ultrasound–Xylanase Treatment
Authors: Lin Yanting , Zhang Siling , Huang Yifei , Yang Shuyuan , Zhou An , Zhang Wencheng , Wu Zeyu
Published: January 2025

The main aim of this study was to enhance the content of soluble dietary fiber (SDF) derived from rice bran (RB) through various treatments, including physical methods (ultrasound and alternating magnetic field (AMF)) and enzymatic approaches (cellulase and xylanase), applied individually or in combination. The results revealed that AMF treatment was the most effective single modification technique for increasing SDF yield, followed by treatments with xylanase, cellulase, and ultrasound. Notably, among the combined approaches, the sequential ultrasound–xylanase treatment (U-X) demonstrated the highest potential for enhancing SDF yield. Further optimization experiments revealed that under the conditions of a xylanase addition of 4.3 mg/g sample, a material-to-liquid ratio of 50 mL/g, and an ultrasonic power of 72 W, the yield of U-X-SDF significantly increased from 1.03% to 18.4%. Compared to unmodified samples, the modified SDF groups exhibited marked enhancements in water holding capacity (42.5–86.4%) and water solubility (21.0–30.6%), while the unmodified SDF displayed superior oil holding capacity than the modified groups. In summary, the sequential ultrasound–xylanase treatment not only improves the SDF yield but also enhances the functional properties of RB-derived SDF, positioning it as a valuable health-promoting food additive with potential benefits for both laboratory and industrial food applications. The optimized treatment process can contribute to the development of new functional food ingredients from RB, thereby promoting health and wellness in consumers.
Comparison of the Thermal Behavior and Chemical Composition of Milk Powders of Animal and Plant Origin
Authors: Dippong Thomas , Muresan Laura Elena , Senila Lacrimioara
Published: January 2025

The present study aims to perform a comparative analysis of the chemical composition and thermal behavior of two distinct milk types, namely animal and plant-based. The thermal analysis revealed the presence of the following classes of compounds: hydrocarbons, heterocycles, aldehydes, ketones, amines and alcohols. All types of milk contain saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), though the relative proportions of these vary depending on the specific milk type. Animal milk powders contain SFAs, including palmitic, stearic, and myristic acids, as well as moderate amounts of MUFAs, such as oleic and palmitoleic acids. They also contain lower PUFAs, including linoleic and alpha-linolenic acids. In contrast, plant-based milk powders, particularly soy milk powder, are rich in both linoleic and alpha-linolenic acids. Plant-based milk typically exhibits lower levels of SFAs and higher levels of MUFAs and PUFAs when compared to milk of animal origin. In conclusion, the fatty acid profiles of animal and plant-based milk powders reflect the different nutritional attributes and health implications associated with each. Thermal behavior analysis offers insights into the stability and potential flavor changes that may occur during processing and storage. The comparative analysis highlights significant differences in the chemical composition and thermal behavior of animal and plant-based milk powders.
Effects of Conjugation with Basil Seed Gum on Physicochemical, Functional, Foaming, and Emulsifying Properties of Albumin, Whey Protein Isolate and Soy Protein Isolate
Authors: Hashemi Hadi , Eskandari Mohammad Hadi , Khalesi Mohammadreza , Golmakani Mohammad-Taghi , Niakousari Mehrdad , Hosseini Seyed Mohammad Hashem
Published: January 2025

Protein conjugation with the Maillard reaction has received considerable attention in the past decades in terms of improving functional properties. This study evaluated the changes in the techno-functional properties of whey protein isolate (WPI), soy protein isolate (SPI), and albumin (Alb) after conjugation with basil seed gum (BSG). The conjugates were developed via the Maillard reaction. Various analyses including FT-IR, XRD, SEM, SDS-PAGE, DSC, RVA, rheology, zeta potential, emulsion, and foaming ability were used for evaluating conjugation products. Conjugation between proteins (WPI, SPI, Alb) and BSG was validated by FT-IR spectroscopy. XRD results revealed a decrease in the peak of BSG after conjugation with proteins. SDS-PAGE demonstrated the conjugation of WPI, SPI, and Alb with BSG. DSC results showed that conjugation with BSG reduced the Tg of WPI, SPI, and Alb from 210.21, 207.21, and 210.90 °C to 190.30, 192.91, and 196.66 °C, respectively. The emulsion activity and emulsion stability of protein/BSG conjugates were increased significantly. The droplet size of emulsion samples ranged from 112.1 to 239.3 nm on day 3. Nanoemulsions stabilized by Alb/BSG conjugate had the smallest droplet sizes (112.1 and 143.3 nm after 3 and 17 days, respectively). The foaming capacity of WPI (78.57%), SPI (61.91%), and Alb (71.43%) in their mixtures with BSG increased to 107.14%, 85.71%, and 85.71%, respectively, after making conjugates with BSG. The foam stability of WPI (39.34%), SPI (61.57%), and Alb (53.37%) in their mixtures with BSG (non-conjugated condition) increased to 77.86%, 77.91%, and 72.32%, respectively, after formation of conjugates with BSG. Conjugation of BSG to proteins can improve the BSG applications as a multifunctional stabilizer in pharmaceutical and food industries.
Frankfurters Manufactured with Valorized Grape Pomace as a Substitute of Nitrifying Salts
Authors: Martín-Mateos María Jesús , Delgado-Adámez Jonathan , Díaz-Ponce María , Tejerina David , Ramírez-Bernabé María Rosario
Published: January 2025

This study investigated the use of grape/wine pomace as a potential substitute for nitrifying salts in the production and preservation of frankfurters. Red wine pomace (RWP) from Tempranillo and white wine pomace (WWP) from Cayetana grapes were added to frankfurters made with Iberian pig backfat—an underutilized fat rich in oleic acid—at two levels (0.5% and 3% w/w). These new formulations were compared with a control (containing only meat, salt, and spices) and a commercial formulation containing nitrites and ascorbic acid. Analyses were conducted immediately after production and following 45 days of refrigerated storage to evaluate microbiological, color, physicochemical, and textural changes in the frankfurters. The addition of pomace slightly reduced the pH of the frankfurters but did not affect microbial counts during the manufacturing process. Frankfurters with pomace displayed a similar color to the control but showed lower redness compared to the commercial formulation with nitrites. Importantly, pomace reduced lipid and protein oxidation during production and storage. The reduction in lipid oxidation due to the pomace was comparable to the effect of nitrites and ascorbic acid. Furthermore, pomace effectively reduced protein oxidation, unlike nitrites and ascorbic acid, which primarily targeted lipid oxidation. Significant differences in texture were observed between commercial frankfurters and those containing pomace. Despite these variations in the appearance and the texture, the strong protective effect of pomace against oxidative reactions highlights its potential as a natural alternative to synthetic additives, offering a promising solution for the meat industry.
Construction of the Closed Form Wave Solutions for TFSMCH and (1 + 1) Dimensional TFDMBBM Equations via the EMSE Technique
Authors: Asaduzzaman Md. , Jesmin Farhana
Published: January 2025

The purpose of this study is to investigate a series of novel exact closed form traveling wave solutions for the TFSMCH equation and (1 + 1) dimensional TFDMBBM equation using the EMSE technique. The considered FONLEEs are used to delineate the characteristic of diffusion in the creation of shapes in liquid beads arising in plasma physics and fluid flow and to estimate the external long waves in nonlinear dispersive media. These equations are also used to characterize various types of waves, such as hydromagnetic waves, acoustic waves, and acoustic gravity waves. Here, we utilize the Caputo-type fractional order derivative to fractionalize the considered FONLEEs. Some trigonometric and hyperbolic trigonometric functions have been used to represent the obtained closed form traveling wave solutions. Furthermore, here, we reveal that the EMSE technique is a suitable, significant, and dominant mathematical tool for finding the exact traveling wave solutions for various FONLEEs in mathematical physics. We draw some 3D, 2D, and contour plots to describe the various wave behaviors and analyze the physical consequence of the attained solutions. Finally, we make a numerical comparison of our obtained solutions and other analogous solutions obtained using various techniques.
Dynamic Analysis of a 10-Dimensional Fractional-Order Hyperchaotic System Using Advanced Hyperchaotic Metrics
Authors: Sarfraz Muhammad , Zhou Jiang , Islam Mazhar , Rasheed Akhter , Liu Qi
Published: January 2025

In this paper, we propose an innovative approach to fractional-order dynamics by introducing a 10-dimensional (10D) chaotic system that leverages the intrinsic memory characteristic of the Grünwald–Letnikov (G-L) derivative. We utilize Lyapunov exponents as a quantitative measure to characterize hyperchaotic behavior, and classify the nature of the suggested 10D fractional-order system (FOS). While several methods exist for calculating Lyapunov exponents (LEs) through the utilization of integer-order systems, these approaches are not applicable for FOS due to its non-local nature. Initially, the system dynamics are thoroughly examined through Lyapunov exponents and bifurcation analysis, considering the influence of both state variables and fractional orders. To assess the hyperchaotic behavior of the proposed model, sensitivity analyses are conducted by exploring changes in state variables under two distinct initial conditions, along with time history simulations for various parameter settings. Furthermore, we examine the impact of different fractional-order sets on the system’s dynamics. A comprehensive performance comparison is conducted between the proposed 10-dimensional fractional-order hyperchaotic system and several existing hyperchaotic systems. This comparison utilizes advanced metrics, including the Kolmogorov–Sinai (KS) entropy, Kaplan–Yorke dimension, the Perron effect analysis, and the 0-1 test for chaos. Simulation outcomes reveal that the proposed system surpasses existing algorithms, delivering improved precision and accuracy.
Hermite–Hadamard-Type Inequalities for Harmonically Convex Functions via Proportional Caputo-Hybrid Operators with Applications
Authors: Butt Saad Ihsan , Umar Muhammad , Khan Dawood , Seol Youngsoo , Tipurić-Spužević Sanja
Published: January 2025

In this paper, we aim to establish new inequalities of Hermite–Hadamard (H.H) type for harmonically convex functions using proportional Caputo-Hybrid (P.C.H) fractional operators. Parameterized by α, these operators offer a unique flexibility: setting α=1 recovers the classical inequalities for harmonically convex functions, while setting α=0 yields inequalities for differentiable harmonically convex functions. This framework allows us to unify classical and fractional cases within a single operator. To validate the theoretical results, we provide several illustrative examples supported by graphical representations, marking the first use of such visualizations for inequalities derived via P.C.H operators. Additionally, we demonstrate practical applications of the results by deriving new fractional-order recurrence relations for the modified Bessel function of type-1, which are useful in mathematical modeling, engineering, and physics. The findings contribute to the growing body of research in fractional inequalities and harmonic convexity, paving the way for further exploration of generalized convexities and higher-order fractional operators.
Decoding the Dialog Between Plants and Arbuscular Mycorrhizal Fungi: A Molecular Genetic Perspective
Authors: Díaz Vanessa , Villalobos Maite , Arriaza Karem , Flores Karen , Hernández-Saravia Lucas P. , Velásquez Alexis
Published: January 2025

Arbuscular mycorrhizal (AM) symbiosis, a mutually beneficial interaction between plant roots and AM fungi, plays a key role in plant growth, nutrient acquisition, and stress tolerance, which make it a major focus for sustainable agricultural strategies. This intricate association involves extensive transcriptional reprogramming in host plant cells during the formation of arbuscules, which are specialized fungal structures for nutrient exchange. The symbiosis is initiated by molecular signaling pathways triggered by fungal chitooligosaccharides and strigolactones released by plant roots, which act as chemoattractants and signaling molecules to promote fungal spore germination, colonization, and arbuscule development. Calcium spiking, mediated by LysM domain receptor kinases, serves as a critical second messenger in coordinating fungal infection and intracellular accommodation. GRAS transcription factors are key components that regulate the transcriptional networks necessary for arbuscule development and maintenance, while small RNAs (sRNAs) from both plant and fungi, contribute to modifications in gene expression, including potential bidirectional sRNA exchange to modulate symbiosis. Understanding the molecular mechanisms related to AM symbiosis may provide valuable insights for implementation of strategies related to enhancing plant productivity and resilience.
A Double-Gene Metabarcoding Approach for the Authentication of Shrimp Surimi-Based Products
Authors: Hu Jiajie , Giusti Alice , Zhang Jixiang , Tinacci Lara , Zhao Chenyang , Ying Xiaoguo , Armani Andrea , Guidi Alessandra , Deng Shanggui
Published: January 2025

Background/Objectives: Shrimp surimi-based products (SSPs) are composed of minced shrimp meat and are highly susceptible to food fraud as fish surimi. This study employed a double-gene metabarcoding approach to authenticate SSPs sold on Chinese e-commerce platforms. Methods: 16S rRNA and 12S rRNA genes were amplified and sequenced from 24 SSPs. Mislabeling was evaluated based on the correspondence between the ingredients (only those of animal origin) reported on the products’ labels and the molecular results. Results: Overall, 87.50% of SSPs (21/24) were found to be mislabeled. The replacement of Penaeus vannamei with other shrimp species was particularly noteworthy. Interestingly, in some SSPs, the primary species detected in terms of sequence abundance were not shrimp but fish, pork, chicken, and cephalopods, raising concerns regarding both health risks and ethical issues related to SSP consumption. The 12S rRNA sequencing results revealed that fish species like Gadus chalcogrammus, Evynnis tumifrons, and Priacanthus arenatus were added to some SSPs in significant proportions, with certain products relying on fish priced from “Low” to “High” levels to substitute higher-cost shrimp. Notably, many fish species in SSPs were highly vulnerable to fishing, raising sustainability concerns. Overall, the high mislabeling rate in SSPs, as well as the detection of endangered fish species (Pangasianodon hypophthalmus), underscores significant quality control issues. Conclusions: DNA metabarcoding has proven to be an effective tool for ingredient authentication in processed seafood.
Vacuolar (H+)-ATPase Genes Are Essential for Cuticle and Wing Development in Locusta migratoria
Authors: Liu Xiaojian , Liang Xiaoyu , Shi Xuekai , Zhang Jianzhen
Published: January 2025

Background/Objectives: Vacuolar (H+)-ATPases (V-ATPases) are crucial in several significant biological processes, including intracellular transport, endocytosis, autophagy and protein degradation. However, their role in the growth and development of insects remains largely unknown. This study aimed to explore the molecular and functional properties of V-ATPases in Locusta migratoria. Methods: LmV-ATPase genes were identified based on the locust transcriptome database and bioinformatics analysis. Quantitative reverse-transcription polymerase chain reaction was used to assess the relative expression of LmV-ATPases in different tissues and developmental stages. RNA interference combined with hematoxylin–eosin staining and transmission electron microscopy was used to explore the functions of LmV-ATPases. Results: Ten V-ATPase genes were identified in L. migratoria and were named LmV-ATPase A, B, C, D, E, F, G, c″, d and e, respectively. These genes were highly expressed in the head, integument, gastric caecum, midgut, hindgut, fat body, trachea and ovary. The transcripts of LmV-ATPases were expressed in the developmental stages examined (from the 3rd to 5th instar nymphs). The injection of double-stranded RNA (dsRNA) against each LmV-ATPase induced high silencing efficiency in the 3rd instar nymphs. Knockdown of LmV-ATPases resulted in lethal phenotypes, with visible defects of the wing and cuticle. We further demonstrated that the deformation was caused by the defects of epidermal cells and fewer new cuticles. Conclusions: These findings suggest that LmV-ATPases are required for the wing and cuticle development of L. migratoria, which could be potential targets for the control of locusts.
Association Between Euthyroidism and Muscular Parameters in Adults with an Excess of Fat Mass: A Preliminary Study
Authors: Greco Francesca , Sicilia Luciana , Seminara Giuseppe , Iuliano Stefano , Tocci Vera , Brunetti Antonio , Aversa Antonio , Di Luigi Luigi , Sgrò Paolo
Published: January 2025

Background/Objectives: Thyroid hormones (THs) are correlated with body composition regardless of the presence of thyroid dysfunctions, whereas little is known regarding THs’ influence on muscular fitness components. This cross-sectional study investigated whether THs may affect muscular parameters in adults who are overweight and affected obesity. Methods: One hundred and five volunteers (65 females and 40 males, BMI: 33.5 (8.0) kg/m2) in an euthyroid state were enrolled. Body composition was evaluated using bioelectrical impedance analysis. Muscular parameters of interest were grip strength—evaluated using the handgrip test (HG) and muscular performance –evaluated using the 10-repetition chair-stand test (CST). Free-triiodothyronine (FT3), free-thyroxine (FT4) and their ratio (FT3/FT4) were evaluated to assess thyroid function. Results: In the female group, the Pearson linear regression analysis revealed a positive correlation between FT3 and HG (R = 0.261, p = 0.036) and a negative correlation between FT3/FT4 and CST (R= −0.266, p= 0.032). In the male group, a positive correlation between FT3 and skeletal muscle mass was found (R = 0.354, p = 0.025). No correlations were found between THs of interest and adiposity indexes (BMI, fat mass) in either group (p > 0.05). Conclusion: FT3 and FT3/FT4 ratio levels in euthyroidism may influence muscular parameters differently in females and males affected by an excess of fat mass. More research is needed to understand the mechanisms behind this correlation and whether THs could be a potential biomarker for muscle-related parameters.
Healing Through Humanized Care: Lessons from a Patient-Centered Perinatal Loss Protocol
Authors: Caro-Costa Rosana , Alcaide-Leyva José Manuel , García-Luque Lourdes , Alcaraz-Clariana Sandra , Guzmán-García José Miguel , Jiménez-Mérida Maria del Rocío
Published: January 2025

Background/Objectives: Perinatal loss is a challenging experience that significantly impacts families and healthcare professionals. This study aimed to explore the perceptions and experiences of the nursing team in the gynecology unit at a Spanish hospital regarding the implementation of a new protocol for perinatal bereavement care. Methods: A qualitative descriptive study was conducted involving a focal group interview with nursing staff from the gynecology unit. Participants were selected through purposive sampling, and data were analyzed using thematic analysis to identify recurring patterns and insights. Results: The findings revealed a disparity in attitudes toward perinatal bereavement care, with some nurses demonstrating empathy and understanding, while others expressed discomfort and avoidance behaviors. Barriers to effective care included a lack of formal training, insufficient emotional support, and practical challenges in implementing the protocol. Participants suggested improvements such as enhanced communication training, the appointment of a bereavement coordinator, and earlier psychological interventions to support both families and staff. Conclusions: The study highlights the need for continuous education and emotional support to facilitate the adoption of perinatal bereavement protocols. Involving nursing staff in protocol development and addressing systemic barriers can improve the quality of care for bereaved families while supporting healthcare professionals’ emotional well-being.
Intangible Capital: Digital Colors in Romanesque Cloisters
Authors: Rossi Adriana , Gonizzi Barsanti Sara , Bertacchi Silvia
Published: January 2025

This paper explores the possibility of counteracting the crisis of culture and institutions by investing in the identity values of the user-actor within digital spaces built for the purpose. The strategy is applied to the analysis of three Catalan cloisters (Spain), with a focus on the representation of the cloister of Sant Cugat (Barcelona). Heuristic picklocks are found in the semantic richness proposed by Marius Schneider exclusively on the verbal level. The authors interpret the contents and transcribe them into graphic signs and digital denotations of sounds and colors. They organize proprietary ontologies, or syntagmatic lines, to be entrusted to the management of computer algorithms. The syncretic culture that characterized the medieval era allowed the ability to mediate science and faith to be entrusted to the mind of the praying monk alone in every canonical hour. The hypothesis that a careful direction has programmed the ways in which to orient souls to “navigate by sight” urges the authors to find the criteria that advanced statistics imitates to make automatic data processing “Intelligent”. In step with the times and in line with the most recent directions for the Safeguarding of Heritage, the musical, astral, and narrative rhythms feared by Schneider are used to inform representative models, to increase not only the visual perception of the user (XR Extended Reality) but also to solicit new analogies and illuminating associations. The results return a vision of the culture of the time suitable for shortening the distances between present and past, attracting the visitor and, with him, the resources necessary to protect and enhance the spaces of the Romanesque era. The methodology goes beyond the contingent aspect by encouraging the ‘remediation’ of contents with the help of machine learning.
The Use of Stone Resources in the Roman Architecture of Oderzo (Treviso, Italy)
Authors: Girotto Chiara , Dilaria Simone , Previato Caterina , Bonetto Jacopo , Mazzoli Claudio
Published: January 2025

This paper presents the results of the analyses conducted on 46 stone samples collected from Roman buildings in Oderzo, a small town located in the heart of the eastern Venetian plain (29 samples), and from architectural artifacts preserved at the local archeological museum “Eno Bellis” (17 samples). The aim of this study is to identify the types and provenance of the stones used for architectural purposes in Roman times in the city of Oderzo (ancient Opitergium). All the materials were petrographically characterized using a multi-analytical approach, including polarized light optical microscopy (PLM). Moreover, volcanic rock samples were analyzed via X-ray fluorescence (XRF) and quantitative phase analysis via X-Ray powder diffraction (QPA-XRPD) to obtain more detailed mineralogical and geochemical characterizations. These methods proved valuable for better determining the provenance of the materials. The results allowed us to determine the quarrying areas that Opitergium mostly relied upon in antiquity for sourcing building materials, as well as the stone trade networks in which the city was integrated. Preliminary findings indicate a higher frequency of stones sourced from outcrops along the Prealpine Arc of north-eastern Italy and Istria, including Aurisina limestone (Trieste Karst), and micritic limestones possibly quarried in the Istrian peninsula for architectural artifacts. Conversely, lithotypes from north-western Prealps appear to have been used less frequently. The volcanic rock samples were entirely sourced from various quarry sites in the Euganean Volcanic District in the Veneto region.
The Effect of Extraction Technique on the Yield, Extraction Kinetics and Antioxidant Activity of Black Pepper (Piper nigrum L.) Ethanolic Extracts
Authors: Milenković Aleksandra , Aleksovski Slavčo , Miteva Karmina , Milenković Lidija , Stanojević Jelena , Nikolić Goran , Ilić Zoran S. , Stanojević Ljiljana
Published: January 2025

This study examines the effect of extraction techniques (maceration-M, reflux-RE, ultrasonic-UE, Soxhlet extraction-SE) on the yield of total extractive matter (TEM), extraction kinetics, and antioxidant activity of black pepper fruits ethanolic extracts (BPFEEs). The content of total phenols and flavonoids was determined by Folin-Ciocalteu and AlCl3 methods, respectively. The antioxidant activity of the extracts was determined by five tests (DPPH, ABTS, FIC, FRAP, and ferricyanide assay), that react by different mechanisms. The highest yield of TEM was observed in the extract obtained by SE (18.77 g/100 g p.m.). Model Ponomarev and a non-stationary diffusion model through the plant material were used for modelling the extraction process. The extract obtained by UE showed the highest content of phenols (85.64 mg GAE/g d.e.), while the extract obtained by RE showed the best antioxidant activity according to DPPH, ABTS, FIC, and FRAP tests, while the extract obtained by UE showed the best activity according to the ferricyanide test. The study provides a comparative analysis of extraction techniques, highlighting their specific advantages, and guiding proper selection based on the desired goal (maximum TEM yield, highest phenolic and flavonoid content, and best antioxidant activity).
Spatial Distribution Characteristics and Relationships of Salt-Based Ions and Nutrients in Old Protected Vegetable Fields
Authors: Zhan Nanbiao , Yang Haotian , Li Jiayang , Shi Xiaodi , Yang Binhao , Sun Yuhang , Guo Gengzi , Cui Xiumin
Published: January 2025

To achieve a scientific and objective evaluation of soil acidification, secondary salinization, and nutrient imbalance in old protected vegetable fields (OPVs) with over 30 years of cultivation history, a soil surface breeding vigorous moss was investigated. Here, quantitative laboratory analysis and mathematical statistics were employed to explore the spatial distribution of soil salinity and nutrients, as well as their relationships. The results revealed that OPVs exhibited slightly acidified values. The measured anions and cations in the soil salt composition constituted approximately 77% of the total ions. Among which, Ca2+ was the dominant cation, while SO42− and NO3− were predominant anions. The total water-soluble salt (TDS) content of the surface soil reached 4.52 g kg−1, exceeding the Chinese Saline Soils standard (1.0 g kg−1) by 350%. In the OPVs, nitrate nitrogen was significantly higher than ammonium nitrogen, and available phosphorus and available potassium were generally abundant. Despite exhibited various soil health concerns, a field visit survey presented consistently high and stable yields in OPVs. We hypothesize that this seemingly contradictory finding may be attributable to several factors, including the abundance of divalent cations (Ca2+ and Mg2+), the soil fertility and water retention capacity of unsaturated salt-based suitable soil, as well as good soil aggregate structure. These factors had the potential to reduce the stresses on the soil. This study provided a foundational understanding of the nutrient and salinity status of soils in OPVs, offering valuable data and theoretical groundwork for future research endeavors.
Bone Diagenesis and Extremes of Preservation in Forensic Science
Authors: Williams Rhys , Thompson Tim , Orr Caroline , Taylor Gillian
Published: January 2025

Understanding the composition and diagenetic processes of the deposition environment is pivotal to understanding why bone undergoes preservation or diagenesis. This research explores the complex nexus of diagenesis at the extremes of preservation, via the interdependent chemical, and short- and long-term microbial processes that influence diagenesis. These processes include dissolution, ion exchange, hydrolysis, recrystallisation, waterlogging, acidity and alkalinity, soil composition, redox potential, bacterial activity, and microbiome composition. Diagenetic processes are discussed in relation to typical sites and sites with extremes of preservation. Understanding site conditions that impact diagenetic processes is critical to understanding the visual features presented in recovered skeletal material, ensuring an appropriate post-mortem interval is assigned, and for subsequent post hoc analysis of bone.