bluuebunny/crossref_metadata_2025 huggingface dataset:
A Stochastic Multi-Strain SIR Model with Two-Dose Vaccination Rate
Authors: Chang Yen-Chang , Liu Ching-Ti
Published: May 2022

Infectious diseases remain a substantial public health concern as they are among the leading causes of death. Immunization by vaccination can reduce the infectious diseases-related risk of suffering and death. Many countries have developed COVID-19 vaccines in the past two years to control the COVID-19 pandemic. Due to an urgent need for COVID-19 vaccines, the vaccine administration of COVID-19 is in the mode of emergency use authorization to facilitate the availability and use of vaccines. Therefore, the vaccine development time is extraordinarily short, but administering two doses is generally recommended within a specific time to achieve sufficient protection. However, it may be essential to identify an appropriate interval between two vaccinations. We constructed a stochastic multi-strain SIR model for a two-dose vaccine administration to address this issue. We introduced randomness into this model mainly through the transmission rate parameters. We discussed the uniqueness of the positive solution to the model and presented the conditions for the extinction and persistence of disease. In addition, we explored the optimal cost to improve the epidemic based on two cost functions. The numerical simulations showed that the administration rate of both vaccine doses had a significant effect on disease transmission.
Using Locality-Sensitive Hashing for SVM Classification of Large Data Sets
Authors: Gonzalez-Lima Maria D. , Ludeña Carenne C.
Published: May 2022

We propose a novel method using Locality-Sensitive Hashing (LSH) for solving the optimization problem that arises in the training stage of support vector machines for large data sets, possibly in high dimensions. LSH was introduced as an efficient way to look for neighbors in high dimensional spaces. Random projections-based LSH functions create bins so that when great probability points belonging to the same bin are close, the points that are far will not be in the same bin. Based on these bins, it is not necessary to consider the whole original set but representatives in each one of them, thus reducing the effective size of the data set. A key of our proposal is that we work with the feature space and use only the projections to search for closeness in this space. Moreover, instead of choosing the projection directions at random, we sample a small subset and solve the associated SVM problem. Projections in this direction allows for a more precise sample in many cases and an approximation of the solution of the large problem is found in a fraction of the running time with small degradation of the classification error. We present two algorithms, theoretical support, and numerical experiments showing their performances on real life problems taken from the LIBSVM data base.
On the Commutators of Marcinkiewicz Integral with a Function in Generalized Campanato Spaces on Generalized Morrey Spaces
Authors: Ku Fuli , Wu Huoxiong
Published: May 2022

This paper is devoted to exploring the mapping properties for the commutator μΩ,b generated by Marcinkiewicz integral μΩ with a locally integrable function b in the generalized Campanato spaces on the generalized Morrey spaces. Under the assumption that the integral kernel Ω satisfies certain log-type regularity, it is shown that μΩ,b is bounded on the generalized Morrey spaces with variable growth condition, provided that b is a function in generalized Campanato spaces, which contain the BMO(Rn) and the Lipschitz spaces Lipα(Rn) (0<α≤1) as special examples. Some previous results are essentially improved and generalized.
Diverse Forms of Breathers and Rogue Wave Solutions for the Complex Cubic Quintic Ginzburg Landau Equation with Intrapulse Raman Scattering
Authors: Seadawy Aly R. , Zahed Hanadi , Rizvi Syed T. R.
Published: May 2022

This manuscript consist of diverse forms of lump: lump one stripe, lump two stripe, generalized breathers, Akhmediev breather, multiwave, M-shaped rational and rogue wave solutions for the complex cubic quintic Ginzburg Landau (CQGL) equation with intrapulse Raman scattering (IRS) via appropriate transformations approach. Furthermore, it includes homoclinic, Ma and Kuznetsov-Ma breather and their relating rogue waves and some interactional solutions, including an interactional approach with the help of the double exponential function. We have elaborated the kink cross-rational (KCR) solutions and periodic cross-rational (KCR) solutions with their graphical slots. We have also constituted some of our solutions in distinct dimensions by means of 3D and contours profiles to anticipate the wave propagation. Parameter domains are delineated in which these exact localized soliton solutions exit in the proposed model.
Antioxidative Effect of Chlorella Pyrenoidosa Protein Hydrolysates and Their Application in Krill Oil-in-Water Emulsions
Authors: Liu Yujia , Qi Yuli , Wang Qi , Yin Fawen , Zhan Honglei , Wang Han , Liu Bingnan , Nakamura Yoshimasa , Wang Jihui
Published: May 2022

Chlorella pyrenoidosa is an excellent source of protein, and in this research, we assessed the antioxidant and emulsifying effects of Chlorella protein hydrolysate (CPH) using neutral proteases and alkaline proteases, as well as the properties of CPH-derived krill oil-in-water (O/W) emulsions. The CPHs exhibited the ability to scavenge several kinds of free radicals, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), O2−, hydroxyl, and ABTS. Additionally, the CPHs (5 mg/mL) scavenged approximately 100% of the DPPH and ABTS. The CPHs showed similar emulsifying activities to Tween 20 and excellent foaming activities (max FS 74%), which helped to stabilize the krill oil-in-water emulsion. Less than 10 mg/mL CPHs was able to form fresh krill oil-in-water emulsions; moreover, the CPHs (5 mg/mL) in a krill O/W emulsion were homogenous, opaque, and stable for at least 30 days. Based on their inhibitory effects on the peroxide value (POV) and thiobarbituric acid reactive substances (TRABS), the CPHs were found to be able to inhibit lipid oxidation in both emulsifying systems and krill O/W emulsions. Thus, the CPHs could improve superoxide dismutase (SOD) activities by 5- or 10-fold and decrease the high reactive oxygen species (ROS) level caused by the addition of H2O2 in vitro. In conclusion, health-promoting CPHs could be applied in krill oil-in-water emulsions as both emulsifiers and antioxidants, which could help to improve the oxidative and physical stability of emulsions.
Cognitive and Speech Rehabilitation in a Patient Affected by Takotsubo Cardiomyophathy: A Case Report
Authors: Corallo Francesco , Bonanno Lilla , Formica Caterina , Coppola Valentina , Di Cara Marcella , D’aleo Piercataldo , Marino Silvia , Smorto Chiara , Lo Buono Viviana
Published: May 2022

Background and Objectives: Takotsubo Syndrome (TS) constitutes one of the most recent clinical realities in modern cardiology. It is clinically similar to the acute coronary syndrome, in the absence of obstructive coronary artery disease. Case Presentation: We described a case of a female patient affected by TS and left ventricular apical thrombus. Several studies described the cardiological syndrome, overlooking the neuropsychological and psychological outcomes. We aimed to assess the advantages of an integrated, multidisciplinary and multifunctional rehabilitation. Conclusions: This specific training contributed to reducing the tolerance to frustration given by her communication’s difficulty. It has favored a good therapeutic alliance and a good success of the psychotherapeutic path, guaranteeing the reduction of her anxious symptoms and an improvement in the emotive and relational status.
Modification of Precipitate Coarsening Kinetics by Intragranular Nanoparticles—A Phase Field Study
Authors: Fashu Simbarashe , Huang Binting , Wang Nan
Published: May 2022

Precipitate coarsening is a major mechanism responsible for the degradation in mechanical properties of many precipitation-hardened alloys at high temperatures. With recent developments in processing of nanocomposite materials, a substantial volume fraction of inert second phase ceramic nanoparticles can be introduced into the grain interiors of polycrystalline materials. These intragranular nanoparticles can have synergistic effects of impeding dislocation motion and interacting with coarsening precipitates to modify the coarsening rate. In this work, the precipitate coarsening behavior of an alloy in the presence of intragranular inert nanoparticles was studied using the phase field method. Two key measurements of coarsening kinetics, precipitate size distribution and coarsening rate, were found to be affected by the volume fraction and the size of nanoparticles. Two novel mechanisms related to geometric constraints imposed by inter-nanoparticle distance and the blockage of solute diffusion path by nanoparticle–matrix interfaces were proposed to explain the observed changes in precipitate coarsening kinetics. The simulation results in general suggest that the use of small nanoparticles with large number density is effective in slowing down the coarsening kinetics.
VHCF, Tribology Characteristics and UNSM Effects of Bainite and Martensite Spring Steels
Authors: Suh Min Soo , Nahm Seung Hoon , Suh Chang Min , Pyun Young Sik
Published: May 2022

It has been reported that the duplex bainite microstructure obtained by austempering (AT) shows higher strength, ductility and impact toughness than quench and tempered (QT) martensite structure in SAE9254 spring steel. However, there seems to be no research on the very high cycle fatigue (VHCF) and tribology characteristics of bainite structure for durability design of next generation spring steel from the perspective of engineering and industrial applications. This is a follow-up study that quantitatively analyzed the mechanical properties, microstructural deformation characteristics, and impact toughness of bainite and martensite using EBSD (Electron Backscatter Diffraction) and SEM (Scanning Electron Microscope) analyses. In this study, VHCF, HCF, tribology characteristics and UNSM (ultrasonic nanocrystal surface modification) effects under duplex bainite and single martensite microstructures were quantitatively studied and analyzed by fracture mechanics from the engineering and industrial point of view to improve durability and weight reduction in spring steels. The bainite AT and martensite QT specimens showed a 56% and 33% increase in fatigue limit for as received AR specimens. Fisheye cracks in duplex bainite AT specimens are similar to ‘facet internal cracks’ that initiated in the absence of inclusions. Generally fisheye crack fracture mode is preferred in VHCF, but fisheye crack was not found in the QT and the AR specimens at all. The UNSM-treated specimens showed fatigue limits that were about 33~50% higher than the untreated specimens.
Copper-Based Alloys with Optimized Hardness and High Conductivity: Research on Precipitation Hardening of Low-Alloyed Binary CuSc Alloys
Authors: Dölling Julia , Henle Ramona , Prahl Ulrich , Zilly Andreas , Nandi Gerrit
Published: May 2022

Copper alloys, combining optimized strength with high electrical and thermal conductivity, are analyzed in-depth, in order to meet the increasing requirements of today’s and tomorrow’s applications in the electrical and automotive industries. The conducted research analyzes alloys with up to 0.3 wt.% scandium, as an alloying element with limited solubility in copper. For the simultaneous enhancement of mechanical strength and conductivity, precipitation hardening is the conducted process method, accompanied by experimental and simulation-based investigations. Therefore, the influence of aging temperatures, in the range of 350 °C to 500 °C, is analyzed in combination with 25%, 50%, and 75% prior cold deformation. CuSc starts precipitating at 375 °C, without prior cold working, whereas mechanical deformation refines the growing intermetallic precipitates. Higher temperatures improve the formation of precipitates but carry the risk of overaging. The first key achievement is to use a thoroughly examined thermomechanical treatment, investigating the growth of precipitates to reach significantly higher hardness than the benchmark alloy, CuZr0.15. Furthermore, the analyzed CuSc alloys show advantages in the investigated recrystallization behavior, making them, especially, applicable for higher operating temperatures. Future research will assess ternary alloying combinations, to further scoop the latent potential of CuSc alloys.
Heritability of Urinary Amines, Organic Acids, and Steroid Hormones in Children
Authors: Hagenbeek Fiona A. , van Dongen Jenny , Pool René , Harms Amy C. , Roetman Peter J. , Fanos Vassilios , van Keulen Britt J. , Walker Brian R. , Karu Naama , Hulshoff Pol Hilleke E. , Rotteveel Joost , Finken Martijn J. J. , Vermeiren Robert R. J. M. , Kluft Cornelis , Bartels Meike , Hankemeier Thomas , Boomsma Dorret I.
Published: May 2022

Variation in metabolite levels reflects individual differences in genetic and environmental factors. Here, we investigated the role of these factors in urinary metabolomics data in children. We examined the effects of sex and age on 86 metabolites, as measured on three metabolomics platforms that target amines, organic acids, and steroid hormones. Next, we estimated their heritability in a twin cohort of 1300 twins (age range: 5.7–12.9 years). We observed associations between age and 50 metabolites and between sex and 21 metabolites. The monozygotic (MZ) and dizygotic (DZ) correlations for the urinary metabolites indicated a role for non-additive genetic factors for 50 amines, 13 organic acids, and 6 steroids. The average broad-sense heritability for these amines, organic acids, and steroids was 0.49 (range: 0.25–0.64), 0.50 (range: 0.33–0.62), and 0.64 (range: 0.43–0.81), respectively. For 6 amines, 7 organic acids, and 4 steroids the twin correlations indicated a role for shared environmental factors and the average narrow-sense heritability was 0.50 (range: 0.37–0.68), 0.50 (range; 0.23–0.61), and 0.47 (range: 0.32–0.70) for these amines, organic acids, and steroids. We conclude that urinary metabolites in children have substantial heritability, with similar estimates for amines and organic acids, and higher estimates for steroid hormones.
Folate Related Pathway Gene Analysis Reveals a Novel Metabolic Variant Associated with Alzheimer’s Disease with a Change in Metabolic Profile
Authors: Miyan Jaleel , Buttercase Charlotte , Beswick Emma , Miyan Salma , Moshkdanian Ghazaleh , Naz Naila
Published: May 2022

Metabolic disorders may be important potential causative pathways to Alzheimer’s disease (AD). Cerebrospinal fluid (CSF) decreasing output, raised intracranial pressure, and ventricular enlargement have all been linked to AD. Cerebral folate metabolism may be a key player since this is significantly affected by such changes in CSF, and genetic susceptibilities may exist in this pathway. In the current study, we aimed to identify whether any single nucleotide polymorphism (SNPs) affecting folate and the associated metabolic pathways were significantly associated with AD. We took a functional nutrigenomics approach to look for SNPs in genes for the linked folate, methylation, and biogenic amine neurotransmitter pathways. Changes in metabolism were found with the SNPs identified. An abnormal SNP in methylene tetrahydrofolate dehydrogenase 1 (MTHFD1) was significantly predictive of AD and associated with an increase in tissue glutathione. Individuals without these SNPs had normal levels of glutathione but significantly raised MTHFD1. Both changes would serve to decrease potentially neurotoxic levels of homocysteine. Seven additional genes were associated with Alzheimer’s and five with normal ageing. MTHFD1 presents a strong prediction of susceptibility and disease among the SNPs associated with AD. Associated physiological changes present potential biomarkers for identifying at-risk individuals.
Changes in Thyroid Metabolites after Liothyronine Administration: A Secondary Analysis of Two Clinical Trials That Incorporated Pharmacokinetic Data
Authors: Diab Nour , Desale Sameer , Danielsen Mark , Köhrle Josef , Shara Nawar , Jonklaas Jacqueline
Published: May 2022

We examined relationships between thyroid hormone (TH) metabolites in humans by measuring 3,5-diiodothyronine (3,5-T2) and 3-iodothyronamine (3-T1AM) levels after liothyronine administration. In secondary analyses, we measured 3,5-T2 and 3-T1AM concentrations in stored samples from two clinical trials. In 12 healthy volunteers, THs and metabolites were documented for 96 h after a single dose of 50 mcg liothyronine. In 18 patients treated for hypothyroidism, levothyroxine therapy was replaced by daily dosing of 30–45 mcg liothyronine. Analytes were measured prior to the administration of liothyronine weekly for 6 weeks, and then hourly for 8 h after the last liothyronine dose of the study. In the weekly samples from the hypothyroid patients, 3,5-T2 was higher by 0.033 nmol/L with each mcg/dL increase in T4 and 0.24 nmol/L higher with each ng/dL increase in FT4 (p-values = 0.007, 0.0365). In hourly samples after the last study dose of liothyronine, patients with T3 values higher by one ng/dL had 3-T1AM values that were lower by 0.004 nmol/L (p-value = 0.0473); patients with 3,5-T2 higher by one nmol/L had 3-T1AM values higher by 2.45 nmol/L (p-value = 0.0044). The positive correlations between weekly trough levels of 3,5-T2 and T4/FT4 during liothyronine therapy may provide insight into 3,5-T2 production, possibly supporting some production of 3,5-T2 from endogenous T4, but not from exogenous liothyronine. In hourly sampling after liothyronine administration, the negative correlation between T3 levels and 3-T1AM, but positive correlation between 3,5-T2 levels and 3-T1AM could support the hypothesis that 3-T1AM production occurs via 3,5-T2 with negative regulation by T3.
Tannic Acid-Modified Silver Nanoparticles in Conjunction with Contact Lens Solutions Are Useful for Progress against the Adhesion of Acanthamoeba spp. to Contact Lenses
Authors: Padzik Marcin , Chomicz Lidia , Bluszcz Julita , Maleszewska Karolina , Grobelny Jaroslaw , Conn David Bruce , Hendiger Edyta B.
Published: May 2022

Acanthamoeba spp. are amphizoic amoebae that are widely distributed in the environment and capable of entering the human body. They can cause pathogenic effects in different tissues and organs, including Acanthamoeba keratitis (AK), which may result in a loss of visual acuity and blindness. The diagnostics, treatment, and prevention of AK are still challenging. More than 90% of AK cases are related to the irresponsible wearing of contact lenses. However, even proper lens care does not sufficiently protect against this eye disease, as amoebae have been also found in contact lens solutions and contact lens storage containers. The adhesion of the amoebae to the contact lens surface is the first step in developing this eye infection. To limit the incidence of AK, it is important to enhance the anti-adhesive activity of the most popular contact lens solutions. Currently, silver nanoparticles (AgNPs) are used as modern antimicrobial agents. Their effectiveness against Acanthamoeba spp., especially with the addition of plant metabolites, such as tannic acid, has been confirmed. Here, we present the results of our further studies on the anti-adhesion potential of tannic acid-modified silver nanoparticles (AgTANPs) in combination with selected contact lens solutions against Acanthamoeba spp. on four groups of contact lenses. The obtained results showed an increased anti-adhesion activity of contact lens solutions in conjunction with AgTANPs with a limited cytotoxicity effect compared to contact lens solutions acting alone. This may provide a benefit in improving the prevention of amoebae eye infections. However, there is still a need for further studies on different pathogenic strains of Acanthamoeba in order to assess the adhesion of the cysts to the contact lens surface and to reveal a more comprehensive picture of the activity of AgTANPs and contact lens solutions.
DNA Markers for Detection and Genotyping of Xanthomonas euroxanthea
Authors: Silva Kayla Gisela , Martins Leonor , Teixeira Miguel , Pothier Joël F. , Tavares Fernando
Published: May 2022

Xanthomonas euroxanthea is a bacterial species encompassing both pathogenic and non-pathogenic strains and is frequently found colonizing the same host plants as X. arboricola. This presents the need to develop a detection and genotyping assay able to track these bacteria in microbial consortia with other xanthomonads. Eight X. euroxanthea-specific DNA markers (XEA1-XEA8) were selected by comparative genomics and validated in silico regarding their specificity and consistency using BLASTn, synteny analysis, CG content, codon usage (CAI/eCAI values) and genomic proximity to plasticity determinants. In silico, the selected eight DNA markers were found to be specific and conserved across the genomes of 11 X. euroxanthea strains, and in particular, five DNA markers (XEA4, XEA5, XEA6, XEA7 and XEA8) were unfailingly found in these genomes. A multiplex of PCR targeting markers XEA1 (819 bp), XEA8 (648 bp) and XEA5 (295 bp) was shown to successfully detect X. euroxanthea down to 1 ng of DNA (per PCR reaction). The topology of trees generated with the concatenated sequences of three markers (XEA5, XEA6 and XEA8) and four housekeeping genes (gyrB, rpoD, fyuA and acnB) underlined the equal discriminatory power of these features and thus the suitability of the DNA markers to discriminate X. euroxanthea lineages. Overall, this study displays a DNA-marker-based method for the detection and genotyping of X. euroxanthea strains, contributing to monitoring for its presence in X. arboricola-colonizing habitats. The present study proposes a workflow for the selection of species-specific detection markers. Prospectively, this assay could contribute to unveil alternative host species of Xanthomonas euroxanthea; and improve the control of phytopathogenic strains.
The Microbiota–Gut–Brain Axis: Gut Microbiota Modulates Conspecific Aggression in Diversely Selected Laying Hens
Authors: Hu Jiaying , Johnson Timothy A. , Zhang Huanmin , Cheng Heng-Wei
Published: May 2022

The gut microbiota plays an important role in regulating brain function, influencing psychological and emotional stability. The correlations between conspecific aggression, gut microbiota, and physiological homeostasis were further studied in inbred laying chicken lines, 63 and 72, which were diversely selected for Marek’s disease, and they also behave differently in aggression. Ten sixty-week-old hens from each line were sampled for blood, brain, and cecal content. Neurotransmitters, cytokines, corticosterone, and heterophil/lymphocyte ratios were determined. Cecal microbiota compositions were determined by bacterial 16s rRNA sequencing, and functional predictions were performed. Our data showed that the central serotonin and tryptophan levels were higher in line 63 compared to line 72 (p < 0.05). Plasma corticosterone, heterophil/lymphocyte ratios, and central norepinephrine were lower in line 63 (p < 0.05). The level of tumor necrosis factor α tended to be higher in line 63. Faecalibacterium, Oscillibacter, Butyricicoccus, and Bacteriodes were enriched in line 63 birds, while Clostridiales vadin BB60, Alistipes, Mollicutes RF39 were dominated in line 72. From the predicted bacterial functional genes, the kynurenine pathway was upregulated in line 72. These results suggested a functional linkage of the line differences in serotonergic activity, stress response, innate immunity, and gut microbiota populations.
Primary Structure and Coding Genes of Two Pheromones from the Antarctic Psychrophilic Ciliate, Euplotes focardii
Authors: Alimenti Claudio , Candelori Annalisa , Jiang Yaohan , Luporini Pierangelo , Vallesi Adriana
Published: May 2022

In ciliates, diffusible cell type-specific pheromones regulate cell growth and mating phenomena acting competitively in both autocrine and heterologous fashion. In Euplotes species, these signaling molecules are represented by species-specific families of structurally homologous small, disulfide-rich proteins, each specified by one of a series of multiple alleles that are inherited without relationships of dominance at the mat-genetic locus of the germinal micronuclear genome, and expressed as individual gene-sized molecules in the somatic macronuclear genome. Here we report the 85-amino acid sequences and the full-length macronuclear nucleotide coding sequences of two pheromones, designated Ef-1 and Ef-2, isolated from the supernatant of a wild-type strain of a psychrophilic species of Euplotes, E. focardii, endemic to Antarctic coastal waters. An overall comparison of the determined E. focardii pheromone and pheromone-gene structures with their homologs from congeneric species provides an initial picture of how an evolutionary increase in the complexity of these structures accompanies Euplotes speciation.
Preparation of Magnetic Activated Carbon by Activation and Modification of Char Derived from Co-Pyrolysis of Lignite and Biomass and Its Adsorption of Heavy-Metal-Containing Wastewater
Authors: Zhang Xiaofang , Li Yuhuan , He Yang , Kong Debin , Klein Bern , Yin Shenghua , Zhao Hongyu
Published: May 2022

Adsorption with activated carbon (AC) is an important method for the treatment of heavy metal wastewater, but there are still certain challenges in the separation and reuse of activated carbon. The preparation of magnetic activated carbon (MAC) by modifying AC is one of the effective means to realize the separation of AC from solution after the adsorption process. In this work, lignite and poplar leaves were used as raw materials for co-pyrolysis, and the co-pyrolysis char was activated and modified to prepare MAC. The structure and properties were characterized by VSM, N2 adsorption, SEM, XRD, and FT-IR. At the same time, the adsorption performance of MAC on wastewater containing Pb and Cd ions was studied. The results show that the prepared MAC contains Fe3O4, and the saturation magnetization (Ms) of the MAC is 13.83 emu/g; the specific surface area of the MAC is 805.86 m2/g, and the micropore volume is 0.23 cm3/g; the MAC exhibited a good porous structure. When the pH value of the solution was 5, the adsorption time was 120 min, the dosage of MAC was 4 g/L, the initial concentration of Pb ion solution was 50 mg/L, and that of Cd ion solution was 25 mg/L, and the adsorption temperature was 30 °C, the adsorption efficiency of Pb, Cd ions were 84.40 and 78.80%, respectively, and the adsorption capacities were 10.55 and 4.93 mg/g, respectively. The adsorption of Pb and Cd ions by MAC conforms to the Langmuir adsorption model, which is a monolayer adsorption. The adsorption process is mainly chemical adsorption, which can be better described by the pseudo-second-order model. The adsorption thermodynamic analysis showed that the adsorption of Pb and Cd ions by MAC was a spontaneous reaction, and the higher the temperature, the stronger the spontaneity.
Cost Optimization of Reinforced Concrete Section According to Flexural Cracking
Authors: Jelušič Primož
Published: May 2022

A series of distributed flexural cracks develop in reinforced concrete flexural elements under the working load. The control of cracking in reinforced concrete is an important issue that must be considered in the design of reinforced concrete structures. Crack width and spacing are influenced by several factors, including the steel percentage, its distribution in the concrete cross-section, the concrete cover, and the concrete properties. In practice, however, a compromise must be made between cracking, durability, and ease of construction and cost. This study presents the optimal design of a reinforced concrete cross-section, using the optimization method of mixed-integer nonlinear programming (MINLP) and the Eurocode standard. The MINLP optimization model OPTCON was developed for this purpose. The model contains the objective function of the material cost considering the crack width requirements. The crack width requirements can be satisfied by direct calculation or by limiting the bar spacing. Due to the different crack width requirements, two different economic designs of reinforced concrete sections were proposed. The case study presented in this study demonstrates the value of the presented optimization approach. A direct comparison between different methods for modelling cracking in reinforced concrete cross-sections, which has not been done before, is also presented.
Antiviral Activities of Officinaloside C against Herpes Simplex Virus-1
Authors: Xiao Ji , Cai Miaomiao , Wang Yifei , Ding Ping
Published: May 2022

The iridoid compounds in traditional Chinese medicine play a prominent role in their antiviral effects. We previously reported the anti-inflammatory effect of new iridoids from the aerial parts of Morinda officinalis. Nevertheless, several open questions remain to explore the other biological functions of these new iridoid compounds. Herpes simplex virus-1 (HSV-1) is one of the most prevalent pathogens in human beings worldwide and due to limited therapies, mainly with the guanosine analog aciclovir (ACV) and other analogs, the search for new drugs with different modes of action and low toxicity becomes particularly urgent for public health. This study aimed to explore the anti-HSV-1 effects of iridoids from the aerial parts of Morinda officinalis. The dried aerial parts of Morinda officinalis were extracted with 95% ethanol and systematic separation and purification were then carried out by modern column chromatography methods such as silica gel column, RP-ODS column, Sephadex LH-20 gel column, and semi-preparative liquid phase, and the structure of these compounds were identified through the physical and chemical properties and a variety of spectral techniques. The obtained seven new iridoid compounds were screened for antiviral activity on HSV-1 through CCK8 and the cytopathic effect, and then the plaque reduction assay, the anti-fluorescence reporter virus strain replication, and RT-qPCR experiments were carried out to further evaluate the antiviral effect. Seven new iridoid compounds (officinaloside A–G) were identified from the aerial parts of Morinda officinalis, and officinaloside C showed anti-HSV-1 activity. Further functional experiments confirmed that officinaloside C has a significant inhibiting effect on HSV-1 virus plaque formation, viral gene, and protein expression, and fluorescent virus replication. Our findings suggest that officinaloside C has significant inhibitory effects on viral plaque formation, genome replication, and viral protein expression of HSV-1 which implies that officinaloside C exhibits viral activity and may be a promising treatment for HSV-1 infection.
Biochemical and Molecular Investigation of the Effect of Saponins and Terpenoids Derived from Leaves of Ilex aquifolium on Lipid Metabolism of Obese Zucker Rats
Authors: Pachura Natalia , Kupczyński Robert , Lewandowska Kamila , Włodarczyk Maciej , Klemens Marta , Kuropka Piotr , Nowaczyk Renata , Krzystek-Korpacka Małgorzata , Bednarz-Misa Iwona , Sozański Tomasz , Pogoda-Sewerniak Krystyna , Szumny Antoni
Published: May 2022

Ilex paraguariensis, the holly tree, is a plant with recognized biological properties, whose aqueous infusions are known as “Yerba mate”, that regulate lipid metabolism, reduce obesity, and improve brain stimulation. In the present study, the effect of standardized saponin and terpenoid fractions of a European taxon, Ilex aquifolium, on blood biochemical parameters in a rat model of metabolic disorder, (fa/fa) Zucker, are presented. The profiles of the volatile fractions of two species and six European varieties of Ilex were investigated. After selecting the best variety, the saponin and terpenoid fractions were isolated and standardized, and animals were fed 10 mg kg−1 b.w. for 8 weeks. A statistically significant decrease in liver adiposity was observed, confirmed by histology and quantitative identification (gas chromatography–mass spectrometry analyses of hepatic lipids. RT-qPCR analysis of gene expression in the aorta revealed that the administration of the terpenoid fraction downregulated LOX-1, suggesting a reduction in atherosclerotic stimuli. In addition, a statistically significant reduction (p < 0.05) in PPARγ for the saponin fraction was observed in the liver. The expression of the ACAT-1 gene in the liver, responsible for the formation of cholesterol esters, increased significantly in the group receiving the terpenoid fraction compared to the control, which was also confirmed by the analysis of individual blood biochemical parameters. The opposite effect was observed for saponins. Taking the above into account, it is shown for the first time that Ilex aquifolium can be a source of compounds that positively influence lipid metabolism.
Hypoglycemic and Antioxidant Properties of Extracts and Fractions from Polygoni Avicularis Herba
Authors: Zhang Kun , Han Mei , Zhao Xia , Chen Xuelin , Wang Hanlei , Ni Jiyan , Zhang Yumei
Published: May 2022

Our research focused on the hypoglycemic capability and the possible mechanisms of extract and fractions from Polygoni Avicularis Herba (PAH) based on α-glucosidase, α-amylase inhibition assays, glucose uptake experiment, HPLC-MS analysis, and molecular docking experiment. In addition, DPPH, ABTS, and FRAP assays were used for determining the antioxidant capability. The results of total flavonoids and phenolics contents showed that ethyl acetate fraction (EAF) possessed the highest flavonoids and phenolics with values of 159.7 ± 2.5 mg rutin equivalents/g and 107.6 ± 2.0 mg galic acid equivalents/g, respectively. The results of in vitro hypoglycemic activity showed that all samples had effective α-glucosidase inhibition capacities, and EAF possessed the best inhibitory effect with IC50 value of 1.58 ± 0.24 μg/mL. In addition, n-butanol fraction (NBF) significantly promoted the glucose uptake rate of 3T3-L1 adipocytes. HPLC-MS analysis and molecular docking results proved the interactions between candidates and α-glucosidase. The results of antioxidation capacities showed that EAF possessed the best antioxidation abilities with DPPH, ABTS, and FRAP. In summary, the hypoglycemic activity of PAH might be related to the inhibition of α-glucosidase (EAF > PEF > NBF) and the promotion of glucose uptake in 3T3-L1 adipocytes (NBF). Simultaneously, the antioxidation capacity of PAH might be related to the abundant contents of flavonoids and other phenolics (EAF > PEF > NBF).
Essential Oil Content and Compositional Variability of Lavandula Species Cultivated in the Mid Hill Conditions of the Western Himalaya
Authors: Rathore Shalika , Kumar Rakesh
Published: May 2022

The increase in the utilization of Lavandula essential oil in industries led to an impressive rise in the demand for quality essential oils. However, a post-harvest drying of Lavandula species can be a decisive factor to determine the quantity and quality of essential oil. The study was conducted in western Himalayan conditions to assess the essential oil content and composition of two Lavandula species viz., lavender (Lavandula angustifolia Mill.), and lavandin (Lavandula × intermedia Emeric ex Loisel), at four different drying duration (0 h, 24 h, 48 h and 72 h after the harvest). The higher growth attributes viz., plant height (71.7 cm), ear length (8.8 cm), number of spikes (18.1), and number of flowers per ear (47.5) were higher in lavandin, while the number of branches (17.1) was higher in lavender. Essential oil content (%) and moisture reduction (%) were significantly higher at 72 h than at 0 h. The major components of lavender and lavandin essential oil were linalool (33.6–40.5%), linalyl acetate (10.8–13.6%), lavandulyl acetate (2.8–14.5%), and linalyl propionate (5.3–14.1%) in both the Lavandula species. There was a decreasing trend in linalool and an increasing trend in linalyl acetate content in lavandin, with an increase in drying duration up to 72 h; while in lavender, no regular trend was observed in linalool and linalyl acetate content. It was observed that linalool and linalyl acetate levels were the highest at 24 and 0 h of drying in lavender and lavandin, respectively, and essential oil extraction can be done according to the desire of the constituent at varied drying duration.
Sustainable Plant Growth Promotion and Chemical Composition of Pyroligneous Acid When Applied with Biochar as a Soil Amendment
Authors: Jindo Keiji , Goron Travis Luc , Kurebito Soboda , Matsumoto Kazuhiro , Masunaga Tsugiyuki , Mori Kouki , Miyakawa Kazuhiro , Nagao Seiya , Tokunari Takeo
Published: May 2022

The pyrolysis of biomass material results in pyroligneous acid (PA) and biochar, among other by-products. In agriculture, PA is recognized as an antimicrobial agent, bio-insecticide, and bio-herbicide due to antioxidant activity provided by a variety of constituent materials. Application of PA to crop plants and soil can result in growth promotion, improved soil health, and reduced reliance on polluting chemical crop inputs. More detailed information regarding chemical compound content within PA and identification of optimal chemical profiles for growth promotion in different crop species is essential for application to yield effective results. Additionally, biochar and PA are often applied in tandem for increased agricultural benefits, but little is known regarding the optimal proportion of each crop input. This work reports on the effect of combined applications of different proportions of PA (200- and 800-fold dilutions) and chemical fertilizer rates (100%, 75%, 50%, and 0%) in the presence or absence of biochar on Komatsuna (Brassica rapa var. perviridis, Japanese mustard spinach) plant growth. To elucidate the chemical composition of the applied PA, four different spectroscopic measurements of fluorescence excitation were utilized for analysis—excitation-emission matrix, ion chromatography, high-performance liquid chromatography, and gas chromatography-mass spectrometry. It was determined that PA originating from pyrolysis of Japanese pine wood contained different classes of biostimulants (e.g., tryptophan, humic acid, and fulvic acid), and application to Komatsuna plants resulted in increased growth when applied alone, and in different combinations with the other two inputs. Additionally, application of biochar and PA at the higher dilution rate increased leaf accumulation of nutrients, calcium, and phosphorus. These effects reveal that PA and biochar are promising materials for sustainable crop production.
Materials Selection for Antifouling Systems in Marine Structures
Authors: Donnelly Bradley , Sammut Karl , Tang Youhong
Published: May 2022

Fouling is the accumulation of unwanted substances, such as proteins, organisms, and inorganic molecules, on marine infrastructure such as pylons, boats, or pipes due to exposure to their environment. As fouling accumulates, it can have many adverse effects, including increasing drag, reducing the maximum speed of a ship and increasing fuel consumption, weakening supports on oil rigs and reducing the functionality of many sensors. In this review, the history and recent progress of techniques and strategies that are employed to inhibit fouling are highlighted, including traditional biocide antifouling systems, biomimicry, micro-texture and natural components systems, superhydrophobic, hydrophilic or amphiphilic systems, hybrid systems and active cleaning systems. This review highlights important considerations, such as accounting for the effects that antifouling strategies have on the sensing mechanism employed by the sensors. Additionally, due to the specialised requirements of many sensors, often a bespoke and tailored solution is preferential to general coatings or paints. A description of how both fouling and antifouling techniques affect maritime sensors, specifically acoustic sensors, is given.
Revisiting the van der Waals Epitaxy in the Case of (Bi0.4Sb0.6)2Te3 Thin Films on Dissimilar Substrates
Authors: Mulder Liesbeth , Wielens Daan H. , Birkhölzer Yorick A. , Brinkman Alexander , Concepción Omar
Published: May 2022

Ultrathin films of the ternary topological insulator (Bi0.4Sb0.6)2Te3 are fabricated by molecular beam epitaxy. Although it is generally assumed that the ternary topological insulator tellurides grow by van der Waals epitaxy, our results show that the influence of the substrate is substantial and governs the formation of defects, mosaicity, and twin domains. For this comparative study, InP (111)A, Al2O3 (001), and SrTiO3 (111) substrates were selected. While the films deposited on lattice-matched InP (111)A show van der Waals epitaxial relations, our results point to a quasi-van der Waals epitaxy for the films grown on substrates with a larger lattice mismatch.
Genotoxicity of Graphene-Based Materials
Authors: Domenech Josefa , Rodríguez-Garraus Adriana , López de Cerain Adela , Azqueta Amaya , Catalán Julia
Published: May 2022

Graphene-based materials (GBMs) are a broad family of novel carbon-based nanomaterials with many nanotechnology applications. The increasing market of GBMs raises concerns on their possible impact on human health. Here, we review the existing literature on the genotoxic potential of GBMs over the last ten years. A total of 50 articles including in vitro, in vivo, in silico, and human biomonitoring studies were selected. Graphene oxides were the most analyzed materials, followed by reduced graphene oxides. Most of the evaluations were performed in vitro using the comet assay (detecting DNA damage). The micronucleus assay (detecting chromosome damage) was the most used validated assay, whereas only two publications reported results on mammalian gene mutations. The same material was rarely assessed with more than one assay. Despite inhalation being the main exposure route in occupational settings, only one in vivo study used intratracheal instillation, and another one reported human biomonitoring data. Based on the studies, some GBMs have the potential to induce genetic damage, although the type of damage depends on the material. The broad variability of GBMs, cellular systems and methods used in the studies precludes the identification of physico-chemical properties that could drive the genotoxicity response to GBMs.
Defect Density-Dependent pH Response of Graphene Derivatives: Towards the Development of pH-Sensitive Graphene Oxide Devices
Authors: Angizi Shayan , Huang Xianxuan , Hong Lea , Akbar Md Ali , Selvaganapathy P. Ravi , Kruse Peter
Published: May 2022

In this study, we demonstrate that a highly pH-sensitive substrate could be fabricated by controlling the type and defect density of graphene derivatives. Nanomaterials from single-layer graphene resembling a defect-free structure to few-layer graphene and graphene oxide with high defect density were used to demonstrate the pH-sensing mechanisms of graphene. We show the presence of three competing mechanisms of pH sensitivity, including the availability of functional groups, the electrochemical double layer, and the ion trapping that determines the overall pH response. The graphene surface was selectively functionalized with hydroxyl, amine, and carboxyl groups to understand the role and density of the graphene pH-sensitive functional groups. Later, we establish the development of highly pH-sensitive graphene oxide by controlling its defect density. This research opens a new avenue for integrating micro–nano-sized pH sensors based on graphene derivatives into next-generation sensing platforms.
Role of Zirconia in Oxide-Zeolite Composite for Thiolation of Methanol with Hydrogen Sulfide to Methanethiol
Authors: Yang Tinglong , Yao Mengqin , Ma Jun , Chen Peng , Zhao Tianxiang , Yang Chunliang , Liu Fei , Cao Jianxin
Published: May 2022

In this paper, the molecular sieve NaZSM-5 was modified with zirconium dioxide (ZrO2) by a hydrothermal coating process and other methods. By comparing the effects of the crystal phase structure of ZrO2 and the compositing method on the physicochemical properties and catalytic performance of the obtained composites, the structure–performance relationship of these composite catalysts was revealed. The results indicate that in the hydrothermal system used for the preparation of NaZSM-5, Zr4+ is more likely to dissolve from m-ZrO2 than from t-ZrO2, which can subsequently enter the molecular sieve, causing a greater degree of desiliconization of the framework. The larger specific surface area (360 m2/g) and pore volume (0.52 cm3/g) of the m-ZrO2/NaZSM-5 composite catalyst increase the exposure of its abundant acidic (0.078 mmol/g) and basic (0.081 mmol/g) active centers compared with other composites. Therefore, this catalyst exhibits a shorter induction period and better catalytic performance. Furthermore, compared with the impregnation method and mechanochemical method, the hydrothermal coating method produces a greater variety of acid–base active centers in the composite catalyst due to the hydrothermal modifying effect.
Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet
Authors: Effting Pauline S. , Thirupathi Anand , Müller Alexandre P. , Pereira Bárbara C. , Sepa-Kishi Diane M. , Marqueze Luis F. B. , Vasconcellos Franciane T. F. , Nesi Renata T. , Pereira Talita C. B. , Kist Luiza W. , Bogo Maurício R. , Ceddia Rolando B. , Pinho Ricardo A.
Published: May 2022

This study investigates whether ladder climbing (LC), as a model of resistance exercise, can reverse whole-body and skeletal muscle deleterious metabolic and inflammatory effects of high-fat (HF) diet-induced obesity in mice. To accomplish this, Swiss mice were fed for 17 weeks either standard chow (SC) or an HF diet and then randomly assigned to remain sedentary or to undergo 8 weeks of LC training with progressive increases in resistance weight. Prior to beginning the exercise intervention, HF-fed animals displayed a 47% increase in body weight (BW) and impaired ability to clear blood glucose during an insulin tolerance test (ITT) when compared to SC animals. However, 8 weeks of LC significantly reduced BW, adipocyte size, as well as glycemia under fasting and during the ITT in HF-fed rats. LC also increased the phosphorylation of AktSer473 and AMPKThr172 and reduced tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL1-β) contents in the quadriceps muscles of HF-fed mice. Additionally, LC reduced the gene expression of inflammatory markers and attenuated HF-diet-induced NADPH oxidase subunit gp91phox in skeletal muscles. LC training was effective in reducing adiposity and the content of inflammatory mediators in skeletal muscle and improved whole-body glycemic control in mice fed an HF diet.
HIV Latency in Myeloid Cells: Challenges for a Cure
Authors: Chitrakar Alisha , Sanz Marta , Maggirwar Sanjay B. , Soriano-Sarabia Natalia
Published: May 2022

The use of antiretroviral therapy (ART) for Human Immunodeficiency Virus (HIV) treatment has been highly successful in controlling plasma viremia to undetectable levels. However, a complete cure for HIV is hindered by the presence of replication-competent HIV, integrated in the host genome, that can persist long term in a resting state called viral latency. Resting memory CD4+ T cells are considered the biggest reservoir of persistent HIV infection and are often studied exclusively as the main target for an HIV cure. However, other cell types, such as circulating monocytes and tissue-resident macrophages, can harbor integrated, replication-competent HIV. To develop a cure for HIV, focus is needed not only on the T cell compartment, but also on these myeloid reservoirs of persistent HIV infection. In this review, we summarize their importance when designing HIV cure strategies and challenges associated to their identification and specific targeting by the “shock and kill” approach.