bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatio-Temporal Relationship between Land Use Carbon Emissions and Ecosystem Service Value in Guanzhong, China
Authors: Zhang Ruijie , Yu Kanhua , Luo Pingping
Published: January 2024

In the context of escalating global concerns for “carbon neutrality and peak carbon” and the urgent need for ecological conservation, deciphering the spatiotemporal interactions between carbon emissions and the ecosystem service value (ESV) in relation to land use changes becomes critically significant. Identifying areas to bolster ecosystem services and curtail carbon emissions, especially within the Guanzhong urban agglomeration, is crucial for advancing sustainable and low-carbon regional development. The study focuses on the urban agglomeration of Guanzhong, using land use and socio-economic data from three periods between 2010 and 2020. Methods such as grid analysis and bivariate spatial autocorrelation models are employed to explore the temporal and spatial evolution characteristics and interaction patterns of carbon emissions and ESV in relation to land use. The findings reveal: (1) during 2010–2020, the Guanzhong urban agglomeration experienced varied transitions in land use types, marked by a significant net decrease in arable land and net increases in grasslands and urban construction areas. (2) The ESV in the Guanzhong urban agglomeration witnessed a consistent rise, exhibiting a spatial distribution pattern with higher values in the southwest and lower in the northeast. Among the categorized ecosystem service functions, services related to hydrological and climate regulation stood out. (3) The Guanzhong urban agglomeration observed an average annual growth rate of 5.03% in carbon emissions due to land use, with a spatial trend that was higher in the center and tapered towards the periphery. Predominant carbon sources included arable lands and urban construction areas, while forests accounted for 94% of carbon sequestration. (4) A pronounced negative correlation between the ESV and carbon emissions was discerned in Guanzhong. Regions with a stronger correlation were primarily centered in Guanzhong, notably around Xi’an and Baoji. The results emphasize the pivotal role of the primary sector’s qualitative development in harmonizing the ESV and carbon emission dynamics in the Guanzhong urban agglomeration. This research provides valuable insights for optimizing land resource management, aligned with the rural revitalization strategy, streamlining carbon dynamics, bolstering ESV, augmenting carbon sequestration efficiency, and guiding ecological spatial planning.
Spatial Spillover Effects of “New Farmers” on Diffusion of Sustainable Agricultural Practices: Evidence from China
Authors: Liu Min , Zhong Taiyang , Lyu Xiao
Published: January 2024

Sustainable agricultural development has been the policy priority for countries with limited natural resources. New farmers’ emergence and growth have not only helped to address the labor crisis in agriculture to some extent, but they have also improved, integrated, and expanded the agricultural sectors. However, less attention has been paid to the performance of new farmers in adopting sustainable agricultural practices and the related spatial effects. Also, few studies have combined the composite adoption intensity of sustainable agricultural practices with the effects of various specific measures. Therefore, this study constructed the Tobit MLE spatial cross-section regression models to empirically study a sample of 174 farmers. We attempted to analyze the impact of the new farmers group on the diffusion of sustainable agricultural practices as well as farmers’ influence on various sorts of sustainable agricultural practices. The results show that new farmers greatly enhanced the adoption of sustainable agricultural practices, as evidenced by the fact that their adoption level was 69.9% greater than that of traditional farmers. However, they had statistically significant negative spillover effects on their neighboring farmers. For the composite adoption intensity of sustainable agricultural practices, there were also statistically significant negative spillover effects. Additionally, there were notable disparities among the factors influencing farmers’ decisions to adopt various types of sustainable agricultural practices and the spatial effects of those adoptions, particularly in the farm size, the number of plots, and the variety of marketing methods employed by farmers. Therefore, measures need to be proposed to address the barriers to the implementation and diffusion of various types of sustainable agricultural practices. This would increase farmers’ willingness to adopt sustainable agricultural practices and the effectiveness of their motivation to neighboring farmers.
Spatial Optimization Based on the Trade-Off between Ecosystem Conservation and Opportunity Cost of Tarim National Park in Xinjiang, China
Authors: Zhang Xinyuan , Zhang Lu , Zhang Zhiming
Published: January 2024

National parks (NPs) are the flagship protected areas in China’s conservation network and play a key role in the ecological protection of core objects and important natural landscapes. However, the shortage of spatial optimization methods based on quantitative indicators has limited the spatially explicit identification of national parks. Therefore, in this study, we selected the main area of the Tarim River (MTR) in Xinjiang as an example to optimize the spatial boundary of a national park. We constructed an evaluation system based on representativeness, ecological importance, and the foundation of the existing protected area. Subsequently, we comprehensively employed a species distribution model to simulate the habitat of the primary conservation targets. Additionally, we spatially optimized the region using an integer linear model, considering multiple scenarios. The results of this study show that fewer than 30% of the existing protected areas in the MTR protect conservation objects. Using different protection objects and quantitative goals, the spatial optimization results for the eight protection scenarios show that the most effective national park establishment scenario covers a total area of 15,009.3 km2, which is 8157.5 km2 more than the protected area already in place and would include the Tarim Populus National Nature Reserve and Luntai Tarim Populus Forest Park. The opportunity cost to be paid for protection according to this boundary is approximately USD 115.14 million. The protection ratios of each protection object for the expanded park are more than 50%, which is 27.7% higher than the protection effect produced by the existing protected area, so we recommend that Tarim National Park be set up on the basis of this boundary.
Meralgia Paresthetica—An Approach Specific Neurological Complication in Patients Undergoing DAA Total Hip Replacement: Anatomical and Clinical Considerations
Authors: Almasi Jozef , Ambrus Richard , Steno Boris
Published: January 2024

Introduction: Mini-invasive surgical (MIS) approaches to total hip replacement (THR) are becoming more popular and increasingly adapted into practice. THR via the direct anterior approach (MIS DAA) has become a rather controversial topic in hip arthroplasty literature in the last decades. Our retrospective observational study focuses on the prevalence of one approach-specific complication—lateral femoral cutaneous nerve (LFCN) iatrogenic lesion—and tries to clarify the possible pathogenesis of this injury. Methods: This is a retrospective single-cohort observational single-center and single-surgeon study. Our patient records were searched for the period from 2015 to 2017—after a safe period of time after the learning curve for MIS DAA. All intra- and post-operative lesions of the LFCN were recorded. Lesion of the LFCN was confirmed by a neurological examination. Minimum patient follow-up was 2 years. Results: This study involved 417 patients undergoing single-side THR via MIS DAA. Patients were examined on follow-up visits at 6 weeks, 6 months, 1 year, and 2 years after surgery. There were 17 cases of LCFN injury at the 6 weeks early follow-up visit (4.1%). All cases of clinically presenting LFCN injury resolved at the 2-year follow-up ad integrum. Discussion: Possible explanations of such neurological complications are direct iatrogenic injury, vigorous traction, hyperextension, or extreme external rotation of the operated limb. Use of a traction table or concomitant spinal pathology and deformity also play a role. Prevention involves stepwise adaptation of the approach during the learning curve period by attending cadaver lab courses, rational use of traction and hyperextension, and careful surgical technique in the superficial and deep fascial layers. Dynamometers could be used to visualise the limits of manipulation of the operated limb. Conclusions: Neurological complications are not as rare but questionably significant in patients undergoing THR via the DAA. Incidental finding of LFCN injury has no effect on the functional outcome of the artificial joint. It can lead to lower subjective satisfaction of patients with the operation, which can be avoided with careful education and management of expectations of the patients.
The Impact of Chronic Comorbidities on Outcomes in Acute Exacerbations of Idiopathic Pulmonary Fibrosis
Authors: Baig Saqib H. , Yoo Erika J.
Published: January 2024

Introduction: Idiopathic pulmonary fibrosis is a chronic progressive lung disease of unknown cause with a high associated mortality. We aimed to compare the impact of chronic medical conditions on hospital outcomes of patients with acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF). Methods: This was a retrospective cohort study using the NIS database from 2016 to 2018. We included patients aged 60 and older hospitalized in academic medical centers with the diagnoses of IPF and acute respiratory failure. We examined factors associated with hospital mortality and length of stay (LOS) using survey-weighted multivariate logistic and negative binomial regression. Results: Out of 4975 patients with AE-IPF, 665 (13.4%) did not survive hospitalization. There was no difference in the mean age between survivors and non-survivors. Patients were more likely to be male, predominantly white, and have Medicare coverage. Most non-survivors were from households with higher median income. Hospital LOS was longer among non-survivors than survivors (9.4 days vs. 9.8 days; p < 0.001). After multivariate-logistic regression, diabetes was found to be protective (aOR 0.62, 95% CI 0.50–0.77; p < 0.0001) while chronic kidney disease (CKD) conferred a significantly higher risk of death after AE-IPF (aOR 6.85, 95% CI 1.90–24.7; p = 0.00). Our multivariate adjusted negative binomial regression model for LOS identified obesity (IRR 0.85, 95% CI 0.76–0.94; p ≤ 0.00) and hypothyroidism (IRR 0.90, 95% CI 0.83–0.98; p = 0.02) to be associated with shorter hospital LOS. Conclusions: Our results suggest that CKD is a significant contributor to hospital mortality in AE-IPF, and diabetes mellitus may be protective. Obesity and hypothyroidism are linked with shorter hospital LOS among patients hospitalized with AE-IPF in US academic medical centers.
Heterogeneous Expression of Arabidopsis Subclass II of SNF1-Related Kinase 2 Improves Drought Tolerance via Stomatal Regulation in Poplar
Authors: Horvat Borislav , Shikakura Yuhei , Ohtani Misato , Demura Taku , Kikuchi Akira , Watanabe Kazuo N. , Oguchi Taichi
Published: January 2024

Abscisic acid (ABA) is the most important phytohormone involved in the response to drought stress. Subclass II of SNF1-related kinase 2 (SnRK2) is an important signaling kinase related to ABA signal transduction. It regulates the phosphorylation of the target transcription factors controlling the transcription of a wide range of ABA-responsive genes in Arabidopsis thaliana. The transgenic poplars (Populus tremula × P. tremuloides, clone T89) ectopically overexpressing AtSnRK2.8, encoding a subclass II SnRK2 kinase of A. thaliana, have been engineered but almost no change in its transcriptome was observed. In this study, we evaluated osmotic stress tolerance and stomatal behavior of the transgenic poplars maintained in the netted greenhouse. The transgenic poplars, line S22, showed a significantly higher tolerance to 20% PEG treatment than non-transgenic controls. The stomatal conductance of the transgenic poplars tended to be lower than the non-transgenic control. Microscopic observations of leaf imprints revealed that the transgenic poplars had significantly higher stomatal closures under the stress treatment than the non-transgenic control. In addition, the stomatal index was lower in the transgenic poplars than in the non-transgenic controls regardless of the stress treatment. These results suggested that AtSnRK2.8 is involved in the regulation of stomatal behavior. Furthermore, the transgenic poplars overexpressing AtSnRK2.8 might have improved abiotic stress tolerance through this stomatal regulation.
Effect of Speed and Number of Sliding Cycles on the Film Formation Behavior of Fluorine-Based Grease
Authors: Chi Jeong-Bin , Ma Hyuk-Jae , Lee Gyu-Sun , Park Ju-Ho , Kim Dae-Eun
Published: January 2024

Grease, a type of lubricant, finds applications in various mechanical components due to advantages such as shape stability and sealing. However, grease is classified as a non-Newtonian fluid, and its film formation behavior is not fully understood to date due to variations in the mechanism related to the film formation behavior of grease based on factors like the base oil, bleed oil, thickener, and additives. In this study, the film formation behavior of fluorine-based grease was analyzed through the 3D profile of the grease after a friction test. In particular, the film formation behavior of the grease during sliding motion was analyzed. The experimental equipment used was a reciprocating-motion-type experimental equipment. Variations in the 3D profile were observed based on the speed and the cycle, indicating differences in the film formation behavior corresponding to changes in viscosity. In contrast to numerous studies that have observed the film formation behavior of grease under rolling motion, investigating the film formation behavior during the initial cycles of sliding motion will provide a profound understanding of the grease’s film formation process.
Feature Extraction of a Planetary Gearbox Based on the KPCA Dual-Kernel Function Optimized by the Swarm Intelligent Fusion Algorithm
Authors: He Yan , Ye Linzheng , Liu Yao
Published: January 2024

The feature extraction problem of coupled vibration signals with multiple fault modes of planetary gears has not been solved effectively. At present, kernel principal component analysis (KPCA) is usually used to solve nonlinear feature extraction problems, but the kernel function selection and its blind parameter setting greatly affect the performance of the algorithm. For the optimization of the kernel parameters, it is very urgent to study the theoretical modeling to improve the performance of kernel principal component analysis. Aiming at the deficiency of kernel principal component analysis using the single-kernel function for the nonlinear mapping of feature extraction, a dual-kernel function based on the flexible linear combination of a radial basis kernel function and polynomial kernel function is proposed. In order to increase the scientificity of setting the kernel parameters and the flexible weight coefficient, a mathematical model for dual-kernel parameter optimization was constructed based on a Fisher criterion discriminant analysis. In addition, this paper puts forward a swarm intelligent fusion algorithm to increase this method’s advantages for optimization problems, involving the shuffled frog leaping algorithm combined with particle swarm optimization (SFLA-PSO). The new fusion algorithm was applied to optimize the kernel parameters to improve the performance of KPCA nonlinear mapping. The optimized dual-kernel function KPCA (DKKPCA) was applied to the feature extraction of planetary gear wear damage, and had a good identification effect on the fuzzy damage boundary of the planetary gearbox. The conclusion is that the DKKPCA optimized by the SFLA-PSO swarm intelligent fusion algorithm not only effectively improves the performance of feature extraction, but also enables the adaptive selection of parameters for the dual-kernel function and the adjustment of weights for the basic kernel function through a certain degree of optimization; so, this method has great potential for practical use.
An Optimal Hierarchical Control Strategy for 4WS-4WD Vehicles Using Nonlinear Model Predictive Control
Authors: Xu Xuan , Wang Kang , Li Qiongqiong , Yang Jiafu
Published: January 2024

Advanced driving algorithms, control strategies, and their optimization in self-driving vehicles in various scenarios are hotspots in current research; 4WS-4WD (four-wheel steering and four-wheel drive) is another hotspot in the study of new concept models; and the nonlinear dynamic characteristics of self-driving vehicles (AVs) are prominent in the fast cornering mode, which leads to a significant reduction in the accuracy and stability of trajectory tracking. Based on these research backgrounds, this paper proposes a control strategy optimization idea based on the 4WS4WD vehicle and its optimization model. The main content includes the establishment of a 3D vehicle model that takes into account vehicle load transfer and position change, and the establishment of a hierarchical control strategy based on the optimized NMPC and 4WS4WD models. The controller consists of two parts: an upper tracking controller based on the new vehicle model and NMPC, and a lower decoupled controller. The tracking control effect of the algorithmic control strategy based on the model and controller is validated in the high-speed serpentine motion mode and double-shift linear motion mode on the joint simulation platform of Car Sim and Simulink.
Solutions of Umbral Dirac-Type Equations
Authors: Yuan Hongfen , Karachik Valery
Published: January 2024

The aim of this work is to study the method of the normalized systems of functions. The normalized systems of functions with respect to the Dirac operator in the umbral Clifford analysis are constructed. Furthermore, the solutions of umbral Dirac-type equations are investigated by the normalized systems.
Sustainable Rail/Road Unimodal Transportation of Bulk Cargo in Zambia: A Review of Algorithm-Based Optimization Techniques
Authors: Miyoba Fines , Mujuni Egbert , Ndiaye Musa , Libati Hastings M. , Abu-Mahfouz Adnan M.
Published: January 2024

Modern rail/road transportation systems are critical to global travel and commercial transportation. The improvement of transport systems that are needed for efficient cargo movements possesses further challenges. For instance, diesel-powered trucks and goods trains are widely used in long-haul unimodal transportation of heavy cargo in most landlocked and developing countries, a situation that leads to concerns of greenhouse gases (GHGs) such as carbon dioxide coming from diesel fuel combustion. In this context, it is critical to understand aspects such as the use of some parameters, variables and constraints in the formulation of mathematical models, optimization techniques and algorithms that directly contribute to sustainable transportation solutions. In seeking sustainable solutions to the bulk cargo long-haul transportation problems in Zambia, we conduct a systematic review of various transportation modes and related mathematical models, and optimization approaches. In this paper, we provide an updated survey of various transport models for bulk cargo and their associated optimized combinations. We identify key research challenges and notable issues to be considered for further studies in transport system optimization, especially when dealing with long-haul unimodal or single-mode heavy cargo movement in countries that are yet to implement intermodal and multimodal systems.
Cooperative Game-Based Digital Twin Drives Decision Making: Overall Framework, Basic Formalization and Application Case
Authors: Hu Fuwen , Bi Song , Zhu Yuanzhi
Published: January 2024

The emerging progress brought about by Industry 4.0 generates great opportunities for better decision making to cope with increasingly uncertain and complex industrial production. From the perspective of game theory, methods based on computational simulations and methods based on physical entities have their intrinsic drawbacks, such as partially accessible information, uncontrollable uncertainty and limitations of sample data. However, an insight that inspired us was that the digital twin modeling method induced interactive environments to allow decision makers to cooperatively learn from the immediate feedback from both cyberspace and physical spaces. To this end, a new decision-making method was put forward using game theory to autonomously ally the digital twin models in cyberspace with their physical counterparts in the real world. Firstly, the overall framework and basic formalization of the cooperative game-based decision making are presented, which used the negotiation objectives, alliance rules and negotiation strategy to ally the planning agents from the physical entities with the planning agents from the virtual simulations. Secondly, taking the assembly planning of large-scale composite skins as a proof of concept, a cooperative game prototype system was developed to marry the physical assembly-commissioning system with the virtual assembly-commissioning system. Finally, the experimental work clearly indicated that the coalitional game-based twinning method could make the decision making of composite assembly not only predictable but reliable and help to avoid stress concentration and secondary damage and achieve high-precision assembly. Obviously, this decision-making methodology that integrates the physical players and their digital twins into the game space can help them take full advantage of each other and make up for their intrinsic drawbacks, and it preliminarily demonstrates great potential to revolutionize the traditional decision-making methodology.
General Solutions for MHD Motions of Ordinary and Fractional Maxwell Fluids through Porous Medium When Differential Expressions of Shear Stress Are Prescribed on Boundary
Authors: Vieru Dumitru , Fetecau Constantin
Published: January 2024

Some MHD unidirectional motions of the electrically conducting incompressible Maxwell fluids between infinite horizontal parallel plates incorporated in a porous medium are analytically and graphically investigated when differential expressions of the non-trivial shear stress are prescribed on the boundary. Such boundary conditions are usually necessary in order to formulate well-posed boundary value problems for motions of rate-type fluids. General closed-form expressions are established for the dimensionless fluid velocity, the corresponding shear stress, and Darcy’s resistance. For completion, as well as for comparison, all results are extended to a fractional model of Maxwell fluids in which the time fractional Caputo derivative is used. It is proven for the first time that a large class of unsteady motions of the fractional incompressible Maxwell fluids becomes steady in time. For illustration, three particular motions are considered, and the correctness of the results is graphically proven. They correspond to constant or oscillatory values of the differential expression of shear stress on the boundary. In the first case, the required time to reach the steady state is graphically determined. This time declines for increasing values of the fractional parameter. Consequently, the steady state is reached earlier for motions of the ordinary fluids in comparison with the fractional ones. Finally, the fluid velocity, shear stress, and Darcy’s resistance are graphically represented and discussed for the fractional model.
The Effect of Aviva Exercise Intervention on Pain Level and Body Awareness in Women with Primary Dysmenorrhea
Authors: Kovács Zoltán , Atombosiye Ekine , Hegyi Gabriella , Szőke Henrik
Published: January 2024

Background and Objective: Primary dysmenorrhea (PD) is one of the most common clinical disorders in women of reproductive age. Our aim was to examine whether a twice-weekly thirty-minute Aviva exercise intervention could result in improvements in pain level and body awareness in patients with PD. Materials and Methods: In our prospective observational trial, the observation period included two consecutive menstrual cycles and the period of the next menstrual bleeding. The first menstrual bleeding period was the first measurement time (T1), the second was the second measurement time (T2), and the third was the third measurement time (T3) in a total of 78 volunteers. The primary endpoint was the change in the level of menstrual pain according to the Numeric Rating Scale (NRS) questionnaire between the intervention group (IG) and the control group (CG) at T1, T2, and T3. In this study, the secondary outcomes were the differences between the IG and CG regarding the different subscales of the Hungarian version of the Body Awareness Questionnaire (BAQ-H) at T1, T2, and T3; the Borg scale results of the IG; and adherence to the intervention. Statistical tests such as independent-sample t-tests, chi-square tests, Pearson’s linear correlation coefficient, and repeated-measure ANCOVA were used for the analyses. Results: In total, 78 volunteers were enrolled: 40 persons in the IG and 38 in the CG. There was a significant change in the level of menstruation pain according to the NRS questionnaire between the IG and CG (p < 0.001). There was no significant difference between the IG and CG regarding the different subscales of the BAQ-H. Only in the case of the “Note responses or changes in body process” subscale of the BAQ-H was there a trend-like effect from the Aviva exercises (p = 0.086). Conclusions: The Aviva exercise could contribute to pain relief from PD. Regarding body awareness, no significant difference was found between the two groups. Due to the short detection period and prospective observational design, our results are preliminary and need to be confirmed in larger clinical trials.
Recurrent Acute Disseminated Encephalomyelitis Presenting as Conus Medullaris Syndrome: A Case Report
Authors: Lee Dae-Wook , Kang Seok , Kim Nackhwan
Published: January 2024

Acute disseminated encephalomyelitis (ADEM) is an inflammatory demyelinating disorder that typically follows an infection or recent vaccination. Symptoms such as encephalopathy and focal neurological deficits appear weeks after the initial illness, leading to swift and progressive neurological decline. While ADEM in the brain has been well documented, reports of ADEM, specifically in the spinal cord, are relatively limited. A 58-year-old male presented with rapidly progressive bilateral lower extremity tingling, numbness, and mild gait disturbance approximately two days prior to visiting the emergency room. Spinal magnetic resonance imaging revealed a diffuse, longitudinal, high-signal lesion with mild enlargement of the conus and proximal cauda equina. The lesions were predominantly localized in the distal conus and cauda equina, and serial electrodiagnostic studies showed that the lesions progressed toward the proximal conus in tandem with symptom evolution and lacked clear lateralization. The patient was subsequently treated with high-dose steroids for seven days (intravenous methylprednisolone, 1 mg/kg). The patient’s lower extremity weakness gradually improved and he was able to walk independently under supervision three weeks after symptom onset. In this case of spinal ADEM in a middle-aged adult, high-dose steroid treatment led to outstanding neurological recovery from both the initial occurrence and subsequent attacks.
Retinitis Pigmentosa: From Pathomolecular Mechanisms to Therapeutic Strategies
Authors: Vingolo Enzo Maria , Mascolo Simona , Miccichè Filippo , Manco Gregorio
Published: January 2024

Retinitis pigmentosa is an inherited disease, in which mutations in different types of genes lead to the death of photoreceptors and the loss of visual function. Although retinitis pigmentosa is the most common type of inherited retinal dystrophy, a clear line of therapy has not yet been defined. In this review, we will focus on the therapeutic aspect and attempt to define the advantages and disadvantages of the protocols of different therapies. The role of some therapies, such as antioxidant agents or gene therapy, has been established for years now. Many clinical trials on different genes and mutations causing RP have been conducted, and the approval of voretigene nepavorec by the FDA has been an important step forward. Nonetheless, even if gene therapy is the most promising type of treatment for these patients, other innovative strategies, such as stem cell transplantation or hyperbaric oxygen therapy, have been shown to be safe and improve visual quality during clinical trials. The treatment of this disease remains a challenge, to which we hope to find a solution as soon as possible.
Analysis of Relationships between Metabolic Changes and Selected Nutrient Intake in Women Environmentally Exposed to Arsenic
Authors: Sijko-Szpańska Monika , Kozłowska Lucyna
Published: January 2024

Nutrients involved in the metabolism of inorganic arsenic (iAs) may play a crucial role in mitigating the adverse health effects associated with such exposure. Consequently, the objective of this study was to analyze the association between the intake levels of nutrients involved in iAs metabolism and alterations in the metabolic profile during arsenic exposure. The study cohort comprised environmentally exposed women: WL (lower total urinary arsenic (As), n = 73) and WH (higher As, n = 73). The analysis included urinary untargeted metabolomics (conducted via liquid chromatography–mass spectrometry) and the assessment of nutrient intake involved in iAs metabolism, specifically methionine, vitamins B2, B6, and B12, folate, and zinc (based on 3-day dietary records of food and beverages). In the WL group, the intake of all analyzed nutrients exhibited a negative correlation with 5 metabolites (argininosuccinic acid, 5-hydroxy-L-tryptophan, 11-trans-LTE4, mevalonic acid, aminoadipic acid), while in the WH group, it correlated with 10 metabolites (5-hydroxy-L-tryptophan, dihyroxy-1H-indole glucuronide I, 11-trans-LTE4, isovalerylglucuronide, 18-oxocortisol, 3-hydroxydecanedioic acid, S-3-oxodecanoyl cysteamine, L-arginine, p-cresol glucuronide, thromboxane B2). Furthermore, nutrient intake demonstrated a positive association with 3 metabolites in the WL group (inosine, deoxyuridine, glutamine) and the WH group (inosine, N-acetyl-L-aspartic acid, tetrahydrodeoxycorticosterone). Altering the intake of nutrients involved in iAs metabolism could be a pivotal factor in reducing the negative impact of arsenic exposure on the human body. This study underscores the significance of maintaining adequate nutrient intake, particularly in populations exposed to arsenic.
Evaluation of a New Standardized Nasal Sampling Method for Detection of SARS-CoV-2 RNA via RT-PCR
Authors: Koeleman Johannes G. M. , Mol Sander , Brand Henk , Ong David S. Y.
Published: January 2024

The aim of this study was to compare the diagnostic accuracy of nasal sampling using a novel anterior nasal swab (ANS) (Rhinoswab) versus combined oro-nasopharyngeal (OP/NP) sampling in COVID-19 suspected patients. This prospective observational study was performed from 11 November to 2 December 2021 (part 1), and from 16 January to 22 February 2022 (part 2). Adult patients who attended the emergency room with suspected COVID-19 were asked to participate. One ANS and one OP/NP sample were consecutively collected, and both were analyzed via reverse transcription polymerase chain reaction (RT-PCR). The result of the OP/NP sample was considered to be the reference standard. A total of 412 patients were included, of whom 171 (41.5%) had a positive RT-PCR of the OP/NP swab, whereas 139 (33.7%) were positive on the ANS sample. The overall diagnostic accuracy for ANS sampling in terms of sensitivity, specificity, positive predictive value, and negative predictive value was 80.7% (95% CI 73.8–86.2), 99.6% (95% CI 97.3–100), 99.3% (95% CI 95.5–100), and 87.9% (95% CI 83.3–91.4), respectively. In conclusion, ANS sampling with the Rhinoswab identified 80.7% of all presented COVID-19 patients in an emergency department. Future studies should investigate if nasal Rhinoswab self-sampling is suitable for reliable diagnosis of COVID-19 in an outpatient setting.
Deconstructing the Dimensions of Mycobiome Fingerprints in Luohandu Cave, Guilin, Southern China
Authors: Man Bai-Ying , Xiang Xing , Cheng Xiao-Yu , Wang Hong-Mei , Su Chun-Tian , Huang Qi-Bo , Luo Yang , Zhang Chao , Cheng Gang , Ni Yu-Yang , Shao Xing-Hua
Published: January 2024

Subterranean karst caves are windows into the terrestrial subsurface to deconstruct the dimensions of mycobiome fingerprints. However, impeded by the constraints of remote locations, the inaccessibility of specimens and technical limitations, the mycobiome of subterranean karst caves has remained largely unknown. Weathered rock and sediment samples were collected from Luohandu cave (Guilin, Southern China) and subjected to Illumina Hiseq sequencing of ITS1 genes. A total of 267 known genera and 90 known orders in 15 phyla were revealed in the mycobiomes. Ascomycota dominated all samples, followed by Basidiomycota and Mortierellomycota. The sediments possessed the relatively highest alpha diversity and were significantly different from weathered rocks according to the diversity indices and richness metrics. Fifteen families and eight genera with significant differences were detected in the sediment samples. The Ca/Mg ratio appeared to significantly affect the structure of the mycobiome communities. Ascomycota appeared to exert a controlling influence on the mycobiome co-occurrence network of the sediments, while Ascomycota and Basidiomycota were found to be the main phyla in the mycobiome co-occurrence network of weathered rocks. Our results provide a more comprehensive dimension to the mycobiome fingerprints of Luohandu cave and a new window into the mycobiome communities and the ecology of subterranean karst cave ecosystems.
Patterns of Spatial Variation in Rumen Microbiology, Histomorphology, and Fermentation Parameters in Tarim wapiti (Cervus elaphus yarkandensis)
Authors: Huang Jianzhi , Sheng Yueyun , Xue Pengfei , Yu Donghui , Guan Peng , Ren Jiangang , Qian Wenxi
Published: January 2024

The rumen is divided into multiple rumen sacs based on anatomical structure, and each has its unique physiological environment. Tarim wapiti preserved roughage tolerance after domestication, and adaptation to the desertified environment led to the development of a unique rumen shape and intraruminal environment. In this work, six Tarim wapiti were chosen and tested for fermentation parameters, microbes, and histomorphology in four rumen areas (Dorsal sac, DS; Ventral sac, VS; Caudodorsal blind sac, CDBS; Caudoventral blind sac, CVBS). Tarim wapiti’s rumen blind sac had better developed rumen histomorphology, the ventral sac was richer in VFAs, and the dominant bacteria varied most notably in the phylum Firmicutes, which was enriched in the caudoventral blind sac. The ventral sac biomarkers focused on carbohydrate fermentation-associated bacteria, the dorsal sac focused on N recycling, and the caudoventral blind sac identified the only phylum-level bacterium, Firmicutes; we were surprised to find a probiotic bacterium, Bacillus clausii, identified as a biomarker in the ventral sac. This research provides a better understanding of rumen fermentation parameters, microorganisms, and histomorphology in the Tarim wapiti rumen within a unique ecological habitat, laying the groundwork for future regulation targeting the rumen microbiota and subsequent animal production improvement.
Megasphaera elsdenii: Its Role in Ruminant Nutrition and Its Potential Industrial Application for Organic Acid Biosynthesis
Authors: Cabral Luciano da Silva , Weimer Paul J.
Published: January 2024

The Gram-negative, strictly anaerobic bacterium Megasphaera elsdenii was first isolated from the rumen in 1953 and is common in the mammalian gastrointestinal tract. Its ability to use either lactate or glucose as its major energy sources for growth has been well documented, although it can also ferment amino acids into ammonia and branched-chain fatty acids, which are growth factors for other bacteria. The ruminal abundance of M. elsdenii usually increases in animals fed grain-based diets due to its ability to use lactate (the product of rapid ruminal sugar fermentation), especially at a low ruminal pH (<5.5). M. elsdenii has been proposed as a potential dietary probiotic to prevent ruminal acidosis in feedlot cattle and high-producing dairy cows. However, this bacterium has also been associated with milk fat depression (MFD) in dairy cows, although proving a causative role has remained elusive. This review summarizes the unique physiology of this intriguing bacterium and its functional role in the ruminal community as well as its role in the health and productivity of the host animal. In addition to its effects in the rumen, the ability of M. elsdenii to produce C2–C7 carboxylic acids—potential precursors for industrial fuel and chemical production—is examined.
Dupilumab Alters Both the Bacterial and Fungal Skin Microbiomes of Patients with Atopic Dermatitis
Authors: Umemoto Naoka , Kakurai Maki , Matsumoto Takanao , Mizuno Kenta , Cho Otomi , Sugita Takashi , Demitsu Toshio
Published: January 2024

The skin microbiome at lesion sites in patients with atopic dermatitis (AD) is characterized by dysbiosis. Although the administration of dupilumab, an IL-4Rα inhibitor, improves dysbiosis in the bacterial microbiome, information regarding the fungal microbiome remains limited. This study administered dupilumab to 30 patients with moderate-to-severe AD and analyzed changes in both fungal and bacterial skin microbiomes over a 12-week period. Malassezia restricta and M. globosa dominated the fungal microbiome, whereas non-Malassezia yeast species increased in abundance, leading to greater microbial diversity. A qPCR analysis revealed a decrease in Malassezia colonization following administration, with a higher reduction rate observed where the pretreatment degree of colonization was higher. A correlation was found between the group classified by the Eczema Area and Severity Index, the group categorized by the concentration of Thymus and activation-regulated chemokine, and the degree of skin colonization by Malassezia. Furthermore, an analysis of the bacterial microbiome also confirmed a decrease in the degree of skin colonization by the exacerbating factor Staphylococcus aureus and an increase in the microbial diversity of the bacterial microbiome. Our study is the first to show that dupilumab changes the community structure of the bacterial microbiome and affects the fungal microbiome in patients with AD.
High Prevalence of Novel Sequence Types in Streptococcus pneumoniae That Caused Invasive Diseases in Kuwait in 2018
Authors: Mokaddas Eiman , Asadzadeh Mohammad , Syed Shabeera , Albert M. John
Published: January 2024

Background: Multilocus sequence typing (MLST) is used to gain insight into the population genetics of bacteria in the form of sequence type (ST). MLST has been used to study the evolution and spread of virulent clones of Streptococcus pneumoniae in many parts of the world. Such data for S. pneumoniae are lacking for the countries of the Arabian Peninsula, including Kuwait. Methods: We determined the STs of all 31 strains of S. pneumoniae from invasive diseases received at a reference laboratory from various health centers in Kuwait during 2018 by MLST. The relationship among the isolates was determined by phylogenetic analysis. We also determined the serotypes by Quellung reaction, and antimicrobial susceptibility by Etest, against 15 antibiotics belonging to 10 classes. Results: There were 28 STs among the 31 isolates, of which 14 were new STs (45.2%) and 5 were rare STs (16.1%). Phylogenetic analysis revealed that 26 isolates (83.9%) were unrelated singletons, and the Kuwaiti isolates were related to those from neighboring countries whose information was gleaned from unpublished data available at the PubMLST website. Many of our isolates were resistant to penicillin, erythromycin, and azithromycin, and some were multidrug-resistant. Virulent serotype 8-ST53, and serotype 19A with new STs, were detected. Conclusions: Our study detected an unusually large number of novel STs, which may indicate that Kuwait provides a milieu for the evolution of novel STs. Novel STs may arise due to recombination and can result in capsular switching. This can impact the effect of vaccination programs on the burden of invasive pneumococcal disease. This first report from the Arabian Peninsula justifies the continuous monitoring of S. pneumoniae STs for the possible evolution of new virulent clones and capsular switching.
Synergistic Zinc(II) and Formate Doping of Perovskites: Thermal Phase Stabilization of α-FAPbI3 and Enhanced Photoluminescence Lifetime of FA0.8MA0.2PbI3 up to 3.7 µs
Authors: Hoeksma Merk M. , Williams René M.
Published: January 2024

Adding zinc (II) cations and formate anions improves the thermal phase stability of α-FAPbI3 materials, and the spin-coated thin films of such doped FAPbI3 (produced using MACl) show an increased emission lifetime of up to 3.7 μs on quartz (for FA0.8MA0.2PbI3). This work investigates the effects of zinc and formate on the phase stability and time-resolved photoluminescence of FAPbI3 perovskites for solar cell applications. Perovskite samples with varying concentrations of zinc and formate were made by incorporating different amounts of zinc formate and zinc iodide and were characterized with XRD. Doping levels of 1.7% Zn(II) and 1.0% formate (relative to Pb) seem optimal. The thermal phase stability of the doped perovskite powders (FAPbI3) and thin films (FA0.8MA0.2PbI3) was assessed. XRD of the thin films after 6 months shows only the alpha-phase. The time-resolved photoluminescence spectroscopy of the doped spin-coated perovskite samples (FA0.8MA0.2PbI3 produced using MACl) is reported. The results show that synergy between an anionic and a cationic dopant can take place, making the perovskite thermally more phase-stable (not converting to the yellow delta-phase) with a longer charge carrier lifetime. In order to produce good thin films by spin coating, the use of MACl was essential.
Hierarchical Zeolites Prepared Using a Surfactant-Mediated Strategy: ZSM-5 vs. Y as Catalysts for Friedel–Crafts Acylation Reaction
Authors: Martins Angela , Amaro Beatriz , Santos M. Soledade C. S. , Nunes Nelson , Elvas-Leitão Ruben , Carvalho Ana P.
Published: January 2024

Hierarchical ZSM5 and Y zeolites were prepared through a surfactant-mediated strategy with NH4OH changing the duration of the treatment and the amount of CTAB surfactant and taking as reference multiples of the critical micellar concentration (CMC). The materials were characterized using powder X-ray diffraction, N2 adsorption isotherms at −196 °C, and SEM and TEM microscopy. The catalytic performance was evaluated in Friedel–Crafts acylation of furan with acetic anhydride at 80 °C. The alkaline surfactant-mediated treatment had different effects on the two zeolites. For ZSM5, the CTAB molecular aggregates can hardly diffuse inside the medium-size pores, leading mainly to intercrystalline mesoporosity and increased external surface area, with no positive catalytic impact. On the other hand, for large-pore Y zeolite, the CTAB molecular aggregates can easily diffuse and promote the rearrangement of crystal units around micelles, causing the enlargement of the pores, i.e., intracrystalline porosity. The optimized Y-based sample, treated for 12 h with a CTAB amount 32 times the CMC, shows an increase in product yield and rate constant that was not observed when a higher amount of surfactant was added. The reuse of spent catalysts upon thermal treatment at 400 °C shows a regeneration efficiency around 90%, showing good potentialities for the modified catalysts.
How Aqueous Solvation Impacts the Frequencies and Intensities of Infrared Absorption Bands in Flavin: The Quest for a Suitable Solvent Model
Authors: Le D. P. Ngan , Hastings Gary , Gozem Samer
Published: January 2024

FTIR spectroscopy accompanied by quantum chemical simulations can reveal important information about molecular structure and intermolecular interactions in the condensed phase. Simulations typically account for the solvent either through cluster quantum mechanical (QM) models, polarizable continuum models (PCM), or hybrid quantum mechanical/molecular mechanical (QM/MM) models. Recently, we studied the effect of aqueous solvent interactions on the vibrational frequencies of lumiflavin, a minimal flavin model, using cluster QM and PCM models. Those models successfully reproduced the relative frequencies of four prominent stretching modes of flavin’s isoalloxazine ring in the diagnostic 1450–1750 cm−1 range but poorly reproduced the relative band intensities. Here, we extend our studies on this system and account for solvation through a series of increasingly sophisticated models. Only by combining elements of QM clusters, QM/MM, and PCM approaches do we obtain an improved agreement with the experiment. The study sheds light more generally on factors that can impact the computed frequencies and intensities of IR bands in solution.
Density Functional Calculation and Evaluation of the Spectroscopic Properties and Luminescent Material Application Potential of the N-Heterocyclic Platinum(II) Tetracarbene Complexes
Authors: Xia Bao-Hui , Ma Yin-Si , Bai Fu-Quan
Published: January 2024

A series of reported Pt(II) carbene complexes possibly have the ability to serve as the new generation of blue emitters in luminescent devices because of their narrow emission spectra, high photoluminescence quantum yields (PLQYs), and rigid molecular skeleton. However, the combination of all carbene ligands with different multidentate structures will affect the overall planarity and horizontal dipole ratio to varying degrees, but the specific extent of this effect has not previously been analyzed in detail. In this work, density functional computation is used to study a class of platinum tetracarbene bidentate complexes with similar absorption and emission band characteristics, which is the main reason for the remarkable difference in quantum efficiency due to subtle differences in electronic states caused by different ligands. From the calculation results, the major reason, which results in significantly decrease in quantum efficiency for [Pt(cyim)2]2+, is that [Pt(cyim)2]2+ can reach the non-radiative deactivation metal-centered d-d excited state through an easier pathway compared with [Pt(meim)2]2+. The result, based on changes in the dihedral angle between ligands, can achieve the goal of improving and designing materials by adjusting the degree of the dihedral angle. (meim: bis(1,1′-dimethyl-3,3′-methylene-diimidazoline-2,2′-diylidene); cyim: bis(1,1′-dicyclohexyl-3,3′-methylene-diimidazoline-2,2′-diylidene).