bluuebunny/crossref_metadata_2025 huggingface dataset:
A non-catalytic role for inositol 1,3,4,5,6-pentakisphosphate 2-kinase in the synthesis of ribosomal RNA
Authors: Brehm Maria A. , Wundenberg Torsten , Williams Jason , Mayr Georg W. , Shears Stephen B.
Published: December 2012

Fundamental to the life and destiny of every cell is the regulation of protein synthesis through ribosome biogenesis, which begins in the nucleolus with the production of ribosomal RNA (rRNA). Nucleolar organization is a highly dynamic and tightly regulated process; the structural factors that direct nucleolar assembly and disassembly are just as important in controlling rRNA synthesis as are the catalytic activities that synthesize rRNA. Here, we report that a signaling enzyme, inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IP5K) is also a structural component in the nucleolus. We demonstrate that IP5K has functionally significant interactions with three proteins that regulate rRNA synthesis: protein kinase CK2, TCOF1 and upstream-binding-factor (UBF). Through molecular modeling and mutagenic studies, we identified an Arg-Lys-Lys tripeptide located on the surface of IP5K that mediates its association with UBF. Nucleolar IP5K spatial dynamics were sensitive to experimental procedures (serum starvation or addition of actinomycin D) that inhibited rRNA production. We show that IP5K makes stoichiometrically sensitive contributions to the architecture of the nucleoli in intact cells, thereby influencing the degree of rRNA synthesis. Our study adds significantly to the biological significance of IP5K; previously, it was the kinase activity of this protein that had attracted attention. Our demonstration that IP5K ‘moonlights’ as a molecular scaffold offers an unexpected new example of how the biological sophistication of higher organisms can arise from gene products acquiring multiple functions, rather than by an increase in gene number.
The transcriptional co-regulator Jab1 is crucial for chondrocyte differentiation in vivo
Authors: Chen Dongxing , Bashur Lindsay A. , Liang Bojian , Panattoni Martina , Tamai Keiko , Pardi Ruggero , Zhou Guang
Published: December 2012

The evolutionarily conserved transcriptional cofactor Jab1 plays critical roles in cell differentiation, proliferation, and apoptosis by modulating the activity of diverse factors and regulating the output of various signaling pathways. Although Jab1 can interact with the bone morphogenetic protein (BMP) downstream effector Smad5 to repress BMP signaling in vitro, the role of Jab1 in BMP-mediated skeletogenesis in vivo is still poorly understood. As a key regulator of skeletogenesis, BMP signaling regulates the critical Ihh-Pthrp feedback loop to promote chondrocyte hypertrophy. In this study, we utilized the loxP/Cre system to delineate the specific role of Jab1 in cartilage formation. Strikingly, Jab1 chondrocyte-specific knockout Jab1flox/flox; Col2a1-Cre (cKO) mutants exhibited neonatal lethal chondrodysplasia with severe dwarfism. In the mutant embryos, all the skeletal elements developed via endochondral ossification were extremely small with severely disorganized chondrocyte columns. Jab1 cKO chondrocytes exhibited increased apoptosis, G2 phase cell cycle arrest, and increased expression of hypertrophic chondrocyte markers Col10a1 and Runx2. Jab1 can also inhibit the transcriptional activity of Runx2, a key regulator of chondrocyte hypertrophy. Notably, our study reveals that Jab1 is likely a novel inhibitor of BMP signaling in chondrocytes in vivo. In Jab1 cKO chondrocytes, there was heightened expression of BMP signaling components including Gdf10/Bmp3b and of BMP targets during chondrocyte hypertrophy such as Ihh. Furthermore, Jab1 cKO chondrocytes exhibited an enhanced response to exogenous BMP treatment. Together, our study demonstrates that Jab1 represses chondrocyte hypertrophy in vivo, likely in part by downregulating BMP signaling and Runx2 activity.
Cooperativity between calmodulin-binding sites in Kv7.2 channels
Authors: Alaimo Alessandro , Alberdi Araitz , Gomis-Perez Carolina , Fernández-Orth Juncal , Gómez-Posada Juan Camilo , Areso Pilar , Villarroel Alvaro
Published: December 2012

Among the multiple roles assigned to calmodulin (CaM), controlling the surface expression of Kv7.2 channels by binding to two discontinuous sites is a unique property of this Ca2+ binding protein. Mutations that interfere with CaM binding or the sequestering of CaM prevent this M-channel component from exiting the endoplasmic reticulum (ER), which reduces M-current density in hippocampal neurons, enhancing excitability and offering a rational mechanism to explain some forms of benign familial neonatal convulsions (BFNC). Previously, we identified a mutation (S511D) that impedes CaM binding while allowing the channel to exit the ER, hinting that CaM binding may not be strictly required for Kv7.2 channel trafficking to the plasma membrane. Alternatively, this interaction with CaM might escape detection and, indeed, we now show that the S511D mutant contains functional CaM-binding sites that are not detected by classical biochemical techniques. Surface expression and function is rescued by CaM, suggesting that free CaM in HEK293 cells is limiting and reinforcing the hypothesis that CaM binding is required for ER exit. Within the CaM-binding domain formed by two sites (helix A and helix B), we show that CaM binds to helix B with higher apparent affinity than helix A, both in the presence and absence of Ca2+, and that the two sites cooperate. Hence, CaM can bridge two binding domains, anchoring helix A of one subunit to helix B of another subunit, in this way influencing the function of Kv7.2 channels.
BMP9-regulated angiogenic signaling plays an important role in the osteogenic differentiation of mesenchymal progenitor cells
Authors: Hu Ning , Jiang Dianming , Huang Enyi , Liu Xing , Li Ruidong , Liang Xi , Kim Stephanie H. , Chen Xiang , Gao Jian-Li , Zhang Hongyu , Zhang Wenwen , Kong Yu-Han , Zhang Jiye , Wang Jinhua , Shui Wei , Luo Xiaoji , Liu Bo , Cui Jing , Rogers Mary Rose , Shen Jikun , Zhao Chen , Wang Ning , Wu Ningning , Luu Hue H. , Haydon Rex C. , He Tong-Chuan , Huang Wei
Published: December 2012

Mesenchymal stromal progenitor cells (MSCs) are multipotent progenitors that can be isolated from numerous tissues. MSCs can undergo osteogenic differentiation under proper stimuli. We have recently demonstrated that bone morphogenetic protein 9 (BMP9) is one of the most osteogenic BMPs. As one of the least studied BMPs, BMP9 has been shown to regulate angiogenesis in endothelial cells. However, it is unclear whether BMP9-regulated angiogenic signaling plays any important role in the BMP9-initiated osteogenic pathway in MSCs. Here, we investigate the functional role of hypoxia-inducible factor 1α (HIF1α)-mediated angiogenic signaling in BMP9-regulated osteogenic differentiation of MSCs. We find that BMP9 induces HIF1α expression in MSCs through Smad1/5/8 signaling. Exogenous expression of HIF1α potentiates BMP9-induced osteogenic differentiation of MSCs both in vitro and in vivo. siRNA-mediated silencing of HIF1α or HIF1α inhibitor CAY10585 profoundly blunts BMP9-induced osteogenic signaling in MSCs. HIF1α expression regulated by cobalt-induced hypoxia also recapitulates the synergistic effect between HIF1α and BMP9 in osteogenic differentiation. Mechanistically, HIF1α is shown to exert its synergistic effect with BMP9 by inducing both angiogenic signaling and osteogenic signaling in MSCs. Thus, our findings should not only expand our understanding of the molecular basis behind BMP9-regulated osteoblastic lineage-specific differentiation, but also provide an opportunity to harness the BMP9-induced synergy between osteogenic and angiogenic signaling pathways in regenerative medicine.
Ezh2 maintains a key phase of muscle satellite cell expansion but does not regulate terminal differentiation
Authors: Woodhouse Samuel , Pugazhendhi Dhamayanthi , Brien Patrick , Pell Jennifer M.
Published: December 2012

Tissue generation and repair requires a stepwise process of cell fate restriction to ensure that adult stem cells differentiate in a timely and appropriate manner. A crucial role has been implicated for Polycomb-group (PcG) proteins and the H3K27me3 repressive histone mark in coordinating the transcriptional programmes necessary for this process, but the targets and developmental timing for this repression remain unclear. To address these questions, we generated novel genome-wide maps of H3K27me3 and H3K4me3 in freshly isolated muscle stem cells. These data, together with the analysis of two conditional Ezh2-null mouse strains, identified a critical proliferation phase in which Ezh2 activity is essential. Mice lacking Ezh2 in satellite cells exhibited decreased muscle growth, severely impaired regeneration and reduced stem cell number, due to a profound failure of the proliferative progenitor population to expand. Surprisingly, deletion of Ezh2 after the onset of terminal differentiation did not impede muscle repair or homeostasis. Using these knockout models and the RNA-Seq and ChIP-Seq datasets, we show that Ezh2 does not regulate the muscle differentiation process in vivo. These results emphasise the lineage and cell-type-specific functions of Ezh2 and Polycomb repressive complex 2.
Impairment of TRPC1–STIM1 channel assembly and AQP5 translocation compromise agonist-stimulated fluid secretion in mice lacking caveolin1
Authors: Pani Biswaranjan , Liu Xibao , Bollimuntha Sunitha , Cheng Kwong Tai , Niesman Ingrid R. , Zheng Changyu , Achen Virginia R. , Patel Hemal H. , Ambudkar Indu S. , Singh Brij B.
Published: December 2012

Neurotransmitter regulation of salivary fluid secretion is mediated by activation of Ca2+ influx. The Ca2+-permeable transient receptor potential canonical 1 (TRPC1) channel is crucial for fluid secretion. However, the mechanism(s) involved in channel assembly and regulation are not completely understood. We report that Caveolin1 (Cav1) is essential for the assembly of functional TRPC1 channels in salivary glands (SG) in vivo and thus regulates fluid secretion. In Cav1−/− mouse SG, agonist-stimulated Ca2+ entry and fluid secretion are significantly reduced. Microdomain localization of TRPC1 and interaction with its regulatory protein, STIM1, are disrupted in Cav1−/− SG acinar cells, whereas Orai1–STIM1 interaction is not affected. Furthermore, localization of aquaporin 5 (AQP5), but not that of inositol (1,4,5)-trisphosphate receptor 3 or Ca2+-activated K+ channel (IK) in the apical region of acinar cell was altered in Cav1−/− SG. In addition, agonist-stimulated increase in surface expression of AQP5 required Ca2+ influx via TRPC1 channels and was inhibited in Cav1−/− SG. Importantly, adenovirus-mediated expression of Cav1 in Cav1−/− SG restored interaction of STIM1 with TRPC1 and channel activation, apical targeting and regulated trafficking of AQP5, and neurotransmitter stimulated fluid-secretion. Together these findings demonstrate that, by directing cellular localization of TRPC1 and AQP5 channels and by selectively regulating the functional assembly TRPC1–STIM1 channels, Cav1 is a crucial determinant of SG fluid secretion.
Influence of land use changes on water chemistry in streams in the State of São Paulo, southeast Brazil
Authors: Silva Daniela M.L. , Camargo Plínio B. , Mcdowell William H. , Vieira Ivan , Salomão Marcos S.M.B. , Martinelli Luiz A.
Published: December 2012

Streamwater is affected by several processes in the watershed including anthropogenic activities that result in changes in water quality as well as in the functioning of these stream ecosystems. Therefore, this work aims to evaluate the concentration of major ions (Ca2+, Mg2+, Na+, K+, NH4+, NO3-, NO2-, Cl-, SO4(2-), PO4(3-), HCO3-) in streams in the state of São Paulo (southeast Brazil). The sampling sites are located at undisturbed (ombrophilus dense forest, semideciduous forest and savanna - cerrado) and disturbed areas (pasture, urbanization and sugar cane crops). Streamwater chemistry varied according to land use change and, in general, was higher in disturbed sites. Streams located in undisturbed sites at Ribeira de Iguape/Alto Paranapanema watershed (streams 1, 2 and 3) seem to be regulated by soil characteristics, as the disturbed streams located at the same watershed covered by pasture (stream 7) showed high concentration for the most of the variables. Exception to streams located at Pontal do Paranapanema watershed where both disturbed (stream 8) and undisturbed streams (stream 4 and 5) presented similar patterns for almost all variables measured.
Anatomy and morphometric aspects of the trachea of Saimiri sciureus Linnaeus, 1758: knowledge for emergency procedures
Authors: Pinheiro Luane L. , Lima Ana R. , Muniz José A.P.C. , Imbeloni Aline , Fioreto Emerson T. , Fontes Rodrigo F. , Cabral Rosa , Branco Érika
Published: December 2012

The aim of this work is to study the morphological characteristics of the trachea of Saimiri sciureus through quantification and measurement of the cartilaginous rings, providing information to facilitate the election of more appropriate endotracheal tube, laryngeal mask or tracheostomy tube for anesthetic and emergency procedures, as it is a species of Neotropical primates most commonly used as biological models, and little is known about their morphology. Nine animals were investigated, being 4 adults and 5 young acquired from the Centro Nacional de Primatas (National Primate Center - CENP) - Ananindeua - PA, which died from natural causes and then fixed in aqueous buffered formalin 10%. Saimiri sciureus trachea comprises an average of 32.8 incomplete rings and an average length of 3.74 cm in young animals, while in adults it demonstrated an average of 30.25 rings and average length of 3.67 cm. The shape of the light and its proportion varied along the trachea. Endotracheal tube with a diameter the 2.0 - 2.5mm, laryngeal mask number 1.0 or tracheostomy tube neonatal Shiley number 3.0, can be placed in animals weighing 600g - 1.2 Kg. Given the great importance of the species studied, which is widely used as a biological model, the detailing on the morphology and morphometry of tracheal animal studies provides new approaches needed in respiratory emergency, as well as, facilitates the development of future anesthetic protocols.
Morphological and morphometric variability of the squid Lolliguncula brevis (Mollusca: Cephalopoda) in Brazilian waters: evidence for two species in the western atlantic?
Authors: Zaleski Tânia , Perez José A. Angel , Gandara-Martins Ana luiza
Published: December 2012

Morphological and morphometric variability of the small-sized coastal squid Lolliguncula brevis was assessed along the largest part of its latitudinal range in the southern hemisphere, off the Brazilian coast (8º S - 27º S). A general homogeneity in form was found throughout the entire latitudinal range. In terms of body proportions, no latitudinal gradients were evident, but a few local "morphotypes" could be distinguished. The distinctive egg mass morphology, and size and form features, including a hectocotylus 20-40% longer than the opposing ventral arm and the presence of suckers on the buccal membrane, indicated that Lolliguncula from northern and southern hemispheres might comprise two different taxa.
Essential oil of Mitracarpus frigidus as a potent source of bioactive compounds
Authors: Fabri Rodrigo L. , Coimbra Elaine S. , Almeida Ana C. , Siqueira Ezequias P. , Alves Tânia M.A. , Zani Carlos L. , Scio Elita
Published: December 2012

In our previous work (Fabri et al. 2009), we showed that different extracts of Mitracarpus frigidus had significant antibacterial, antifungal and leishmanicidal activities. In order to increase our knowledge about this species, this work assesses the chemical composition and the in vitro biological activity of its essential oil. Thus, the essential oil obtained by hydrodistillation of the aerial parts of M. frigidus was analyzed by GC/MS. Among several compounds detected, 11 were identified, being linalool and eugenol acetate the major components. The essential oil exhibited a moderate antibacterial effect against Staphyloccocus aureus, Bacillus cereus, Pseudomonas aeruginosa and Enterobacter cloacae (MIC 250 µg/mL). On the other hand, it showed a strong antifungal effect against Cryptoccocus neoformans (MIC 8 µg/mL) and Candida albicans (MIC 63 µg/mL). Expressive activity against L. major and L. amazonensis promastigote forms with IC50 values of 47.2 and 89.7 µg/mL, respectively, were also observed. In addition, the antioxidant activity was investigated through DPPH radical-scavenging and showed a significative activity with IC50 of 38 µg/mL. The cytotoxicity against Artemia salina was moderate with LC50 of 88 µg/mL. The results presented here are the first report on the chemical composition and biological properties of M. frigidus essential oil.
Comparative studies of two dimensional and three dimensional ultrasonic nuchal translucency in trisomy assessments
Authors: Wee Lai K. , Chai Hum Y. , Samsury Sharul R. Bin , Mujamil Naizaithull F. Binti , Supriyanto Eko
Published: December 2012

Current two-dimensional (2D) ultrasonic marker measurements are inherent with intra- and inter-observer variability limitations. The objective of this paper is to investigate the performance of conventional 2D ultrasonic marker measurements and proposed programmable interactive three-dimensional (3D) marker evaluation. This is essentially important to analyze that the measurement on 3D volumetric measurement possesses higher impact and reproducibility vis-à-vis 2D measurement. Twenty three cases of prenatal ultrasound examination were obtained from collaborating hospital after Ethical Committee's approval. The measured 2D ultrasonic marker is Nuchal Translucency or commonly abbreviated as NT. Descriptive analysis of both 2D and 3D ultrasound measurement were calculated. Three trial measurements were taken for each method. Both data were tested with One-Sample Kolmogorov-Smirnov Test and results indicate that markers measurements were distributed normally with significant parametric values at 0.621 and 0.596 respectively. Computed mean and standard deviation for both measurement methods are 1.4495 ± 0.46490 (2D) and 1.3561 ± 0.50994 (3D). ANOVA test shows that computerized 3D measurements were found to be insignificantly different from the mean of conventional 2D at the significance level of 0.05. With Pearson's correlation coefficient value or R = 0.861, the result proves strong positive linear correlation between 2D and 3D ultrasonic measurements. Reproducibility and accuracy of 3D ultrasound in NT measurement was significantly increased compared with 2D B-mode ultrasound prenatal assessment. 3D reconstructed imaging has higher clinical values compare to 2D ultrasound images with less diagnostics information.
Animal model for training in sentinel lymph node biopsy of the stomach through combined methods
Authors: Alves José Roberto , Lopes Luiz Roberto , Meirelles Luciana Rodrigues de , Ramos Celso Darío , Sasasaki Tânia , Andreollo Nelson Adami
Published: December 2012

PURPOSE: Create and validate a proposed animal model for training in sentinel lymph node biopsy of the stomach. METHODS: In thirty-two rabbits, through a laparotomy, they received a subserosal injection of 0.1 ml of phytate labeled with technetium-99m (0.2 mCi) in the anterior wall of the gastric corpus, followed by 0.2 ml of Blue Patent V® 2.5%, through the same puncture site. Suspicious lymph nodes were searched in vivo at five, ten and 20 minutes, both visually (Blue Patent stained lymph nodes) and with a manual gamma radiation detector (to detect suspected radioactive lymph nodes). After 20 minutes, was performed resection of these for further evaluation of radioactivity (ex vivo) and histological study. RESULTS: Lymph nodes were identified in 30 rabbits (Average of 2.2 lymph nodes per animal). Of the 90 suspected lymph nodes that occurred in the study, 70 cases (77.8%) were histologically confirmed for lymphoid tissue. Of these, the majority were located in the periesophageal region of the gastric fundus. The sample presented a mortality rate of 6.25% and nine complications related to the method, which interfered in the identification of the lymph nodes. CONCLUSION: The animal model for sentinel node biopsy in rabbit stomachs proved to be feasible, with low complexity and reproduced the difficulties encountered for gastric lymph node biopsy in humans, being adequate for surgical training.
An experimental study to compare inflammatory response due to liquid or gas joint distension in horses submitted to arthroscopy
Authors: Rossetti Renata Bello , Massoco Cristina de Oliveira , Penna Ana Carolina Alves , Silva Luis Claudio Lopes Correia da
Published: December 2012

PURPOSE: To assess comparatively the inflammatory response that follows CO2 or Ringer's lactate joint capsular distension of horses submitted to experimental arthroscopy METHODS: Each animal was submitted to a bilateral tarsocrural arthroscopy employing gas distention in one joint and fluid distention in the contralateral joint. Synovial fluid was evaluated at 0, six, 12, 24 and 48 hours post-operative. RESULTS: The use of CO2 for arthroscopy causes an acute and mild synovitis alike to the liquid capsular distension, showing similar synovial fluid increase of leukocytes, TP, and TNF-α. Although synovial fluid PGE2 content was higher in joints submitted to CO2 distension, lower levels of hemoglobin and leukocytes oxidative burst after surgery indicates that CO2 arthroscopy decreased intra-articular bleeding and activation of infiltrating leukocytes. CONCLUSIONS: The use of CO2 for arthroscopic examination causes acute and mild synovitis that is similar to the effects caused by the liquid capsular distension. CO2 also seems to decrease intra-articular bleeding and activation of leukocytes.
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells
Authors: Souza Sobrinho Celestino Prospero de , Gragnani Alfredo , Santos Ivan Dunshee Abranches Oliveira , Oliveira Andrea Fernandes , Lipay Monica Vanucci Nunes , Ferreira Lydia Masako
Published: December 2012

PURPOSE: To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS: Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS: After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION: The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation.
Evaluation of the abdominal wall cicatrization of rabbits exposed to nicotine and undergone abdominoplasty using nylon thread or cyanoacrylate
Authors: Costa Luciano Assis , Jardim Paulo dos Reis , Macedo Pedro Henrique Alvares Paiva , Amaral Vânia da Fonseca , Silva Alcino Lázaro da , Barbosa Cirênio de Almeida
Published: December 2012

PURPOSE: To compare the wound healing of the abdominal wall of rabbits exposed to nicotine and submitted to abdominoplasty using 2-octyl cyanoacrylate or nylon thread for the surgery suture. METHODS: Thirty two rabbits were used. They were divided in subgroups: A1, A2, B1 e B2. Group A received saline 0.9%; group B received nicotine, both groups for 14 days before surgery. We performed an abdominoplasty with a nylon suture into the A1 and B1 subgroups; as for A2 and B2 groups the suture was performed with cyanoacrylate. The euthanasia happened in the 14th post-operative day. After, we evaluated: swollen process, fibroblast proliferation, collagen, neovascularization, and macroscope and microscope epithelization of the scars. RESULTS: We observed the presence of eosinophils in all scars exposed to the cyanoacrylate, and a significant increase of neovascularization in the subgroup B2 comparing to the A2 one (p=0.037). The other variables haven't showed any statistical difference. CONCLUSIONS: Nicotine hasn't influenced the swollen process, the fibroblast proliferation, the presence of collagen, neither the epithelialization. The neovascularization showed cicatricial immaturity when comparing group A2 to group B2. The eosinophils in the scars repaired with glue showed that the substance has acted as an allergen.
Wound healing under the effect of iodine cadexomer in rats
Authors: Brustolin Ester Verônica , Skare Thelma Larocca , Nassif Paulo Afonso Nunes , Biondo-Simões Maria de Lourdes Pessole , Prestes Manoel Alberto , Ozono Laila Marim , Ramos Camila Ercolin
Published: December 2012

PURPOSE: To assess vthe action of iodine cadexomer in the healing process of surgical wounds in rats and if cytotoxicity occurs with the systemic absorption of iodine. METHODS: Thirty six Wistar rats were used and performed 53 wounds with surgical punch of 6 mm diameter on them. Two lesions were made diametrically opposed on groups with distilled water (GAD) and sodium chloride (GCS); on the right lesions were used bandage with distilled water and on the left ones dressing with sodium chloride. In cadexomer iodine (GCI) group, a punch injury was made only on the left side and the dressing was carried out with cadexomer iodine. The groups were divided in two sub-groups according to the day of death (7 and 14). Microscopically was used H&E staining, through which the inflammation could be observed and also the neovascularization. Staining with Masson trichrome studied fibrosis. TSH and free T4 were used for absorption recognition of iodine, and its toxic potential was performed before death with the animal anesthetized. RESULTS: Microscopic analysis showed more marked intensity of inflammation in group GAD, subgroup 14 days. Neovascularization showed be discrete in GCS sub-group 14 days. Fibrosis was more pronounced in the group GCI. Comparing the types of treatment, there was statistical significance between groups GCI and GCS (p<0.013). The TSH and T4, showed no difference between the control group and GCI in relation to the absorption of iodine. In evaluating the GCI and control groups, within each treatment, statistical significance was found between them (p<0.001) when compared the days of observation. CONCLUSION: Cadexomer iodine had beneficial effects in all phases of the healing process without cytotoxicity due iodine absorption.
Sciatic nerve regeneration in rats by a nerve conduit engineering with a membrane derived from natural latex
Authors: Ganga Marcos Vinícius Muniz , Coutinho-Netto Joaquim , Colli Benedicto Oscar , Marques Junior Wilson , Catalão Carlos Henrique Rocha , Santana Ricardo Torres , Oltramari Marcos Roberto Pedron , Carraro Kleber Tadeu , Lachat João-José , Lopes Luiza da Silva
Published: December 2012

PURPOSE: To evaluate the capacity of natural latex membrane to accelerate and improve the regeneration quality of the of rat sciatic nerves. METHODS: Forty male adult Wistar rats were used, anesthetized and operated to cut the sciatic nerve and receive an autograft or a conduit made with a membrane derived from natural latex (Hevea brasiliensis). Four or eight weeks after surgery, to investigate motor nerve recovery, we analyzed the neurological function by walking pattern (footprints analysis and computerized treadmill), electrophysiological evaluation and histological analysis of regenerated nerve (autologous nerve graft or tissue cables between the nerve stumps), and anterior tibial and gastrocnemius muscles. RESULTS: All functional and morphological analysis showed that the rats transplanted with latex conduit had a better neurological recovery than those operated with autologous nerve: quality of footprints, performance on treadmill (p<0.01), electrophysiological response (p<0.05), and quality of histological aspects on neural regeneration. CONCLUSION: The data reported showed behavioral and functional recovery in rats implanted with latex conduit for sciatic nerve repair, supporting a complete morphological and physiological regeneration of the nerve.
Hepatic damage in newborns from female rats exposed to the pesticide derivative ethylenethiourea
Authors: Lemos Patrícia Veruska Ribeiro Barbosa , Martins José Luiz , Lemos Sidney Pereira Pinto , Santos Fernando Leandro dos , Silva Sílvio Romero Gonçalves e
Published: December 2012

PURPOSE: To evaluate hepatic morphological-histological abnormalities in newborns from female rats exposed to ethylenethiourea. METHODS: A randomized study was conducted on fifty-five newborn Wistar rats were studied: 34 in the experimental group, whose mothers had been exposed to 1% ethylenethiourea; and 21 in the control group, whose mothers had received 0.9% physiological solution. The solution was administered via gavage on the 11th day of gestation. Cesarean section was performed on the 20th day of gestation. The newborns' livers were examined and any morphological-histological abnormalities were registered. The presence of megakaryocytes was quantified in 50 microscope fields, as the total number of these cells per mm². RESULTS: The entire experimental group presented abnormalities of embryonic formation, with musculoskeletal anomalies, digestive system anomalies, hepatic congestion and friability, hydrops and delayed intrauterine growth. The histopathological analysis showed that morphological-histological hepatic destructuring had occurred in all entire experimental with removal of the hepatic trabeculae and severe hepatic megakaryocytosis. The mean megakaryocyte density ranged from 107.9 to 114.2 per mm², and it was eight times greater than in the control group, thus characterizing a situation of extramedullary hematopoiesis. CONCLUSION: The fetal exposure to ethylenethiourea caused hepatic damage characterized by severe extramedullary hematopoiesis.
The effects of local nitroglycerin on the surgical delay procedure in prefabricated flaps by vascular implant in rats
Authors: Sá Jairo Zacchê de , Aguiar José Lamartine de Andrade , Cruz Adriana Ferreira , Schuler Alexandre Ricardo Pereira , Lima José Ricardo Alves de , Marques Olga Martins
Published: December 2012

PURPOSE: To evaluate the effect of local nitroglycerin on the viable area of a prefabricated flap for vascular implant in rats, and to investigate the surgical delay procedure. METHODS: A femoral pedicle was implanted under the skin of the abdominal wall in forty Wistar rats. The animals were divided into four groups of ten: group 1 - without surgical delay procedure and local nitroglycerin; group 2 - with surgical delay procedure, but without local nitroglycerin; group 3 - without surgical delay procedure, but with local nitroglycerin; and group 4 - with simultaneous surgical delay procedure and local nitroglycerin. The percentages of the viable areas, in relation to the total flap, were calculated using AutoCAD R 14. RESULTS: The mean percentage value of the viable area was 8.9% in the group 1. 49.4% in the group 2; 8.4% in the group 3 and 1.1% in the group 4. There was significant difference between groups 1 and 2 (p=0.005), 1 and 4 (p=0.024), 2 and 3 (p=0.003), 2 and 4 (p=0.001). These results support the hypothesis that the closure of the arterial venous channels is responsible for the phenomenon of surgical delay procedure. CONCLUSION: Local nitroglycerin did not cause an increase in the prefabricated viable flap area by vascular implantation and decreased the viable flap area that underwent delay procedures.
Nursing Activities Score e demanda de trabalho de enfermagem em terapia intensiva
Authors: Leite Illoma Rossany Lima , Silva Grazielle Roberta Freitas da , Padilha Kátia Grillo
Published: December 2012

OBJETIVO: Medir e caracterizar a carga de trabalho de enfermagem em Unidade de Terapia Intensiva (UTI) por meio da aplicação do Nursing Activities Score (NAS). MÉTODOS: Estudo descritivo quantitativo, retrospectivo, realizado em uma das UTIs de um Hospital Filantrópico de Teresina- PI, de setembro a outubro de 2010, com amostra de 66 pacientes. Foram realizadas 285 medidas do escore NAS. RESULTADOS: Quanto à carga de trabalho de enfermagem, foi verificada uma média do escore total do NAS de 68,1% (51,5% e 108,3%), correspondendo à porcentagem de tempo gasto pelo profissional de enfermagem na assistência direta ao paciente nas 24 horas. Houve correlação estatística entre NAS e desfecho clínico (p= 0,001). Já entre NAS e tempo de internação (p= 0,073) e NAS e idade (p=0,952), não houve significância estatística. CONCLUSÃO: Os resultados mostraram que os pacientes apresentaram elevada necessidade de cuidados, refletida pela média elevada do NAS.
Demandas de saúde mental: percepção de enfermeiros de equipes de saúde da família
Authors: Souza Jacqueline de , Luis Margarita Antonia Villar
Published: December 2012

OBJETIVO: Descrever como são identificadas e acolhidas as necessidades de saúde mental (SM) por equipes de saúde da família, conforme a concepção de enfermeiros. MÉTODOS: Estudo exploratório, descritivo de caráter qualitativo. Utilizaram-se entrevistas semiestruturadas junto a cinco enfermeiros e a interpretação foi norteada pelas preconizações do Ministério da Saúde Brasileiro sobre a inclusão das ações de SM na atenção básica. RESULTADOS: Identificou-se que a falta de indicadores no Sistema de Informações da Atenção Básica (SIAB) afeta o planejamento das ações de SM e que outras doenças crônicas, como diabetes e hipertensão, são prioritárias para as equipes. As ações de SM vêm sendo incorporadas gradativamente no processo de trabalho das equipes de saúde da família, e a consultoria de psiquiatria exerce importante papel nisso. CONCLUSÃO: Reconhece-se a necessidade de educação permanente, revisão do SIAB e, sobretudo, criação de projetos terapêuticos sistematizados e à disposição para novos modos de cuidar.
Estilos de manejo familiar: uma possibilidade de avaliação no transplante hepático pediátrico
Authors: Mendes-Castillo Ana Márcia Chiaradia , Bousso Regina Szylit , Santos Maiara Rodrigues dos , Damião Elaine Buchhorn Cintra
Published: December 2012

OBJETIVO: Identificar os estilos de manejo familiar durante a experiência do transplante hepático da criança, de acordo com o Family Management Style Framework. MÉTODOS: Estudo descritivo, com abordagem qualitativa, realizado mediante uma análise secundária de nove entrevistas semiestruturadas, previamente coletadas com oito famílias que tiveram uma criança que atravessava a experiência de transplante hepático. RESULTADOS: Pela análise, foi possível identificar cinco estilos de manejo: família ajustada, família em adaptação, família lutando, família em conflito e família em espera. CONCLUSÃO: O modelo mostrou-se útil na avaliação de famílias no contexto do transplante pediátrico e seu uso é encorajado neste e em outros cenários de doença crônica.
Ocupações exercidas por adolescentes e sua relação com a participação escolar
Authors: Rezende Marina Pereira , Cano Maria Aparecida Tedeschi , Mauro Maria Yvone Chaves , Oliveira Denize Cristina de , Marziale Maria Helena Palucci , Robazzi Maria Lúcia do Carmo Cruz
Published: December 2012

OBJETIVOS: Identificar as ocupações exercidas por adolescentes participantes de um programa social de atendimento para jovens e avaliar sua inserção na escola, após a entrada no mundo do trabalho. MÉTODOS: Estudo descritivo, quantitativo, realizado junto a um programa de apoio e atendimento aos jovens, por meio do qual 437 jovens trabalhadores foram entrevistados. RESULTADOS: A maioria era do gênero masculino (54%), com média de idade de 16 anos, cursando o ensino médio; trabalhavam como office boys (36,4%), auxiliares administrativos (25,4%), recepcionistas (16,0%) e no estacionamento rotativo (12,6%). Atraso escolar foi constatado em 95,8% dos indivíduos, indicando descompasso entre a série cursada e a idade cronológica; entretanto, o desempenho na escola pareceu não ter sido prejudicado pelo trabalho. CONCLUSÃO: Deve-se ter cautela ao introduzir o adolescente no mercado de trabalho, pois aspectos como cansaço, falta de tempo para estudar, entre outros, podem ter implicações nas atividades escolares.
Fatores associados à baixa adesão ao exame colpocitológico em mães adolescentes
Authors: Batista Rosimeire Pereira Bressan , Mastroeni Marco Fabio
Published: December 2012

OBJETIVO: Analisar os fatores associados à baixa adesão ao teste de Papanicolaou em um grupo de mães adolescentes. MÉTODOS: Estudo observacional, transversal realizado em dois hospitais públicos de Joinville, Santa Catarina. A amostra constituiu-se de 416 puérperas adolescentes. Os dados foram coletados no período de março a setembro de 2010 por meio de entrevista, durante a internação hospitalar. RESULTADOS: As adolescentes que apresentaram menor adesão à realização do teste de Papanicolaou foram as com idade inferior a 15 anos, somente estudantes, com baixa escolaridade e renda familiar, que utilizaram o preservativo como método contraceptivo, que possuíam somente um filho, que realizaram menos que seis consultas pré-natais e que não foram solicitadas a fazer o teste durante a gestação. CONCLUSÃO: As variáveis faixa etária, ocupação, escolaridade, anos de estudo, método contraceptivo, paridade, número de consultas pré-natais e a oferta do exame colpocitológico durante a gestação, foram significativamente associadas à realização do teste de Papanicolaou.
Cateter urinário: mitos e rituais presentes no preparo do paciente
Authors: Mazzo Alessandra , Gaspar Aidê Amábile Coelho dos Santos , Mendes Isabel Amélia Costa , Trevizan Maria Auxiliadora , Godoy Simone de , Martins José Carlos Amado
Published: December 2012

OBJETIVO: Este estudo buscou identificar e descrever mitos e rituais no preparo do paciente para a inserção do cateter urinário. MÉTODOS: Trata-se de um estudo observacional, exploratório e descritivo, realizado em nove hospitais de um município do interior paulista. Seguidos os preceitos éticos, os dados foram coletados por entrevista, junto ao enfermeiro designado pela instituição, utilizando-se um instrumento semiestruturado. Dentre as 13 instituições do município, nove participaram do estudo. RESULTADOS: Todos os hospitais possuíam o procedimento de cateterismo urinário padronizado e implantado. Durante o preparo do paciente, 5 (55,5%) não fizeram referência à orientação, privacidade e humanização. Todas adotam procedimentos divergentes para higiene e antissepsia do meato uretral, com presença de mitos e rituais que se sobressaem às evidências científicas e destacam o modelo funcionalista nos procedimentos realizados pela equipe de enfermagem. CONCLUSÃO: Há necessidade de reavaliar a intervenção de cateterismo urinário, com discussões interdisciplinares, dando ênfase à utilização de princípios científicos.
Reincidência da violência contra crianças no Município de Curitiba: um olhar de gênero
Authors: Fonseca Rosa Maria Godoy Serpa da , Egry Emiko Yoshikawa , Nóbrega Caroline Rife , Apostólico Maíra Rosa , Oliveira Rebeca Nunes Guedes de
Published: December 2012

OBJETIVOS: Analisar a reincidência de violência infantil no Município de Curitiba - Paraná e compreender o fenômeno com base na perspectiva de gênero. MÉTODOS: Estudo de abordagem quantitativa do tipo descritivo exploratório. Dentre as 338 notificações de violência contra crianças de zero a 9 anos de idade junto à Rede de Proteção à Criança e ao Adolescente dessa cidade com última notificação em 2009 foram analisados 300 casos por serem reincidentes. RESULTADOS: A totalidade dos casos foi de violência intrafamiliar e a reincidência mais frequente foi a negligência, tendo com principal agressora a mãe. A violência sexual apareceu mais entre as meninas, com casos reincidentes no mesmo tipo ou com a negligência antecedendo. A manifestação da violência reincidente contra crianças apresentou-se sobreposta, recorrente, com tendência a agravar-se com a evolução dos casos. CONCLUSÃO: A violência contra as crianças é determinada por relações de poder determinadas pelas categorias gênero e geração, que produzem iniquidades potencializadoras da vulnerabilidade familiar em relação à violência. O olhar crítico sobre essa questão pode indicar medidas de superação no tocante à assistência e à prevenção de sua ocorrência e reincidência tanto do setor saúde como de outros setores.
Interface entre saúde e meio ambiente na formação profissional em saúde
Authors: Camponogara Silviamar , Diaz Paola da Silva , Rossato Gabriela Camponogara , Peres Roger Rodrigues , Soares Sabrina de Aguiar , Erthal Graciele , Viero Cibelle Mello
Published: December 2012

OBJETIVO: Conhecer a concepção de acadêmicos da área da saúde sobre a interface saúde e meio ambiente, e como isso se expressa em sua vivência acadêmica. MÉTODOS: Estudo de abordagem qualitativa, descritivo-exploratório, realizado com 24 acadêmicos da área da saúde de uma instituição pública de ensino superior. Os dados coletados, no período de agosto a setembro de 2010, por meio de entrevista semi-estruturada foram analisados de acordo com o referencial da análise de conteúdo. RESULTADOS: Os sujeitos relataram compreender que há uma estreita interface entre saúde e meio ambiente. Apontaram que as populações menos favorecidas socioeconomicamente estão mais predispostas aos efeitos da problemática ambiental, o que leva a ocorrência de muitas doenças. Alguns sujeitos, entretanto, apresentaram dificuldade de manifestar uma ideia sobre o tema. CONCLUSÃO: É necessária uma abordagem da temática no processo formativo de profissionais da área da saúde, visando à aquisição de responsabilidade socioambiental.
Assessment of caregiver burden with elderly having cognitive deficit
Authors: Gratao Aline Cristina Martins , Talmelli Luana Flávia da Silva , Haas Vanderlei José , Marques Sueli , Kusumota Luciana , Rodrigues Rosalina Aparecida Partezani
Published: December 2012

To characterize the elderly with cognitive déficit, and family caregivers who live in the urban communit,y and to identify the caregiver burden. METHODS: An observational, cross-sectional study, conducted between January and July 2009. Seventy elderly (aged 65 and older) were assessed for cognitive deficit using the Mini Mental State Examination (MMSE); their 70 caregivers were evaluated by the Zarit Burden Scale (ZBS) and the Self Reporting Questionnaire (SRQ). The data were entered and validated by double entry in the MS Excel Program, and were analyzed using SPSS software, version 15.0. RESULTS: Of the caregivers, 47.1% were children. We obtained a mean for the ZBS of 30.3 (± 17.3) and 6.2 (± 3.2) for the SRQ, p <0.05, correlating lower scores of the MMSE with high scores on ZBS and SRQ. CONCLUSIONS: Cognitive deficit of the elderly was a predictive factor for burden and emotional distress of caregivers. Knowing the reduction of cognitive capacity related to caregiver burden is essential for preserving caregiver health.
Qualidade de vida de egressos de curso de Cuidadores de Idosos
Authors: Bittar Ana Maria , Grillo Luciane Peter , Mariath Aline Brandão , Próspero Elisete Navas Sanches , Sandri Juliana Vieira de Araújo
Published: December 2012

OBJETIVO: Avaliar a qualidade de vida de egressos de Curso de Cuidadores de Idosos. MÉTODOS: Estudo transversal desenvolvido no Centro Formador de Recursos Humanos Caetano Munhoz da Rocha no Estado do Paraná. A amostra constituiu-se de 59 egressos que responderam ao questionário WHOQOL-bref, da Organização Mundial de Saúde. RESULTADOS: A maioria dos cuidadores era do sexo feminino, com idade média de 39,9 ± 10,8 anos, casados ou vivendo como casados e com Ensino Médio completo. Os egressos avaliaram possuir melhor qualidade de vida no domínio relações sociais (75,6 + 16,9), seguida dos domínios psicológicos (71,4 ± 13,2), físicos (69,1 ± 14,2) e meio ambiente (58,6 ± 11,6). CONCLUSÃO: O estudo revelou aspectos relevantes dos cuidadores de idosos e pode fornecer sustentação para implementação de novas estratégias para melhoria da qualidade de vida desses indivíduos.
Sintomas e fatores de risco para asma entre escolares piauienses
Authors: Castro Gessianne Carvalho , Sousa Lívia Kênia de Carvalho , Vera Paula Valentina de Sousa , Lima Luisa Helena de Oliveira , Oliveira Edina Araújo Rodrigues , Lima Rúbia Fernanda Santos , Magalhães Luis Fernando Beserra , Araújo Nileide Lima , Lopes Marcos Venícios de Oliveira
Published: December 2012

OBJETIVOS: Analisar a prevalência de sintomas de asma entre escolares de seis a sete anos do município de Picos-PI; e identificar fatores de risco associados à doença. MÉTODOS: Estudo transversal realizado com crianças de 6 e 7 anos de Picos em novembro de 2010 a maio de 2011. Para coletar os dados, foi utilizado um formulário adaptado do Internacional Study of Asthma and Allergies in Childhood. Os dados foram analisados com base na estatística descritiva e analítica. RESULTADOS: Dentre as 234 crianças da amostra estudada, 56% eram do gênero feminino. A prevalência de diagnóstico médico de asma foi de 7,7%. Os seguintes fatores de risco apresentaram relação estatisticamente significante com a ocorrência de asma (p < 0,05): sibilos após a realização de exercício físico, tosse seca à noite sem estar gripado, bronquite, e ter pais asmáticos. CONCLUSÃO: Observou-se o subdiagnóstico da asma e há necessidade de que novos trabalhos sejam realizados com uma amostra maior, para analisar de modo mais amplo a situação da asma em crianças no município de Picos - PI.
Educação em saúde e a família do bebê prematuro: uma revisão integrativa
Authors: Chiodi Lucilei Cristina , Aredes Natália Del' Angelo , Scochi Carmen Gracinda Silvan , Fonseca Luciana Mara Monti
Published: December 2012

OBJETIVO: Identificar as necessidades de educação em saúde da família do recém-nascido pré-termo. MÉTODOS: Revisão integrativa de literatura, com buscas nas bases de dados PubMED e Literatura Latino-Americana e do Caribe em Ciências da Saúde, entre 1999 e 2011, e com uso dos descritores (prematuro, enfermagem neonatal, cuidados de enfermagem e alta hospitalar) em inglês, espanhol e português. RESULTADOS: Os dez estudos selecionados foram categorizados em três temáticas: a participação nos cuidados do filho, o preparo para a alta hospitalar e uso de material educativo. A maioria das atividades de educação em saúde é direcionada às mães e está relacionada aos cuidados básicos diários e, ainda, é incipiente a participação nestas atividades, a fim de promover o vínculo e aumentar a confiança no cuidado. CONCLUSÃO: Observou-se a necessidade de elaborar estratégias, embasadas nas metodologias ativas de aprendizagem, auxiliadas por materiais educacionais que facilitem a inserção dos pais na unidade neonatal e nos cuidados com seu filho.
Idosos portadores de HIV e vivendo com AIDS no contexto da capacidade funcional
Authors: Cruz Gylce Eloisa Cabreira Panitz , Ramos Luiz Roberto
Published: December 2012

A Síndrome da Imunodeficiência Adquirida (Aids) na velhice é mais uma doença crônica que pode comprometer a autonomia e a independência e afetar a capacidade funcional dos portadores idosos. Nesta perspectiva, este estudo visou a discutir sobre a capacidade funcional dos portadores do Vírus da Imunodeficiência Humana - HIV/Aids com 60 anos ou mais, baseado na incursão da bibliografia brasileira, com a finalidade de estimular novas produções, diminuir a lacuna na literatura e fornecer conhecimento aos profissionais e gestores de serviços e políticas de saúde do idoso.