bluuebunny/crossref_metadata_2025 huggingface dataset:
Signatures of Adaptation in Mitochondrial Genomes of Palearctic Subterranean Voles (Arvicolinae, Rodentia)
Authors: Bondareva Olga , Genelt-Yanovskiy Evgeny , Petrova Tatyana , Bodrov Semen , Smorkatcheva Antonina , Abramson Natalia
Published: December 2021

This study evaluates signatures of selection in the evolution of the mitochondrial DNA of voles, subfamily Arvicolinae, during the colonization of subterranean environments. The comparative sequence analysis of mitochondrial protein-coding genes of eight subterranean vole species (Prometheomys schaposchnikowi, three species of the genus Ellobius: Ellobius talpinus, Ellobius fuscocapillus and Ellobius lutescens, two species of the genus Terricola: Terricola subterraneus and Terricola daghestanicus, Lasiopodomys mandarinus, and Hyperacrius fertilis) and their closest aboveground relatives was applied using codon-substitution models. The highest number of selection signatures was detected in genes ATP8 and CYTB. The relaxation of selection was observed in most mitochondrial DNA protein-coding genes for subterranean species. The largest amount of relaxed genes is discovered in mole voles (genus Ellobius). The number of selection signatures was found to be independent of the evolutionary age of the lineage but fits the degree of specialization to the subterranean niche. The common trends of selective pressures were observed among the evolutionary ancient and highly specialized subterranean rodent families and phylogenetically young lineages of voles. It suggests that the signatures of adaptation in individual mitochondrial protein-coding genes associated with the colonization of the subterranean niche may appear within a rather short evolutionary timespan.
A Prospective Study of Long-Term Regenerative Endodontics Outcomes of Necrotic Immature Permanent Teeth: An 8-Year Follow-Up
Authors: Abu Zeid Sawsan T. , Alamoudi Ruaa A. , Alothmani Osama S. , Mokeem Saleh Abeer A. , Siddiqui Amna Y.
Published: December 2021

For the management of necrotic immature teeth, regenerative endodontics offers the advantage of further root lengthening, thickening of dentin wall, and apical closure. This prospective study aimed to evaluate the long-term outcome of regenerative endodontics in immature necrotic permanent teeth. A total of 23 immature roots were medicated by triple antibiotic paste. After 21 days, bleeding was induced by over-instrumentation, and then mineral trioxide aggregate and coronal restoration were applied. Patients were scheduled for clinical and radiographic follow-up for 8 years. The radiographic changes of root dimensions were assessed using the ImageJ Plugin and statistically analyzed by Kruskal–Wallis test at a 95% confidence level. For qualitative evaluation, images were overlapped and analyzed using Photoshop software. All teeth were asymptomatic one month after the treatment. All teeth (n = 18) with preoperative periapical radiolucency showed complete resolution within 6–9 months. Recall rate at two, three, and eight years was 69.6%, 56.5%, and 34.8%, respectively. Continuous root development with a significant increase in root length and thickening of dentin wall accompanied by a significant decrease in apical canal diameter was seen at the end of the observation period (p < 0.001). In conclusion, the long-term outcome of regenerative endodontics revealed successful clinical and radiographic results with appropriate case selection.
Using Sigmoid Growth Curves to Establish Growth Models of Tomato and Eggplant Stems Suitable for Grafting in Subtropical Countries
Authors: Hsieh Chih-Yu , Fang Shih-Lun , Wu Yea-Fang , Chu Yung-Chu , Kuo Bo-Jein
Published: December 2021

In subtropical regions, tomato (Solanum lycopersicum) is mainly produced in autumn and winter. To enhance the off-season production of tomato, summer cultivation has become a prime objective. Grafting tomato scions onto eggplant (Solanum melongena) rootstocks is a key method to overcome the difficulties of tomato cultivation in summer. In this study, we collected seedling growth data over six growing seasons in Taiwan and established growth models by employing three commonly used sigmoid growth curves, namely the Gompertz, Richards, and Logistic curves. Cumulative temperature was introduced as an independent variable and its relationship with plant stem diameter determined. The R2 values of the growth models were 0.74–0.85 and 0.72–0.80 in calibration and validation, respectively. Performance did not differ markedly among models in the same growing season, but notable differences were observed among models for different growing seasons. In addition, the estimates of several model parameters differed significantly among the seasons; hence, separate models should be established for different seasons. The results of this study can be used in prediction of tomato and eggplant seedling growth and arrangement of the grafting schedule to improve the efficiency of seedling production in subtropical countries.
Quality Attributes of Chitosan-Coated Cornelian Cherry (Cornus mas L.) Fruits under Different Storage Temperatures
Authors: Ebrahimzadeh Asghar , Esmaeili Maryam , Hassanpour Hamid , Hassanpouraghdam Mohammad Bagher , Ercisli Sezai , Bozhuyuk Mehmet Ramazan , Dokoupil Libor , Mlcek Jiri
Published: December 2021

Temperature is the dominant environmental stimulus that influences the postharvest quality, visual appearance, and nutritional content of fruits. Temperature hastens fruit ripening and senescence by the impact on respiration rate and the acceleration of metabolic reactions. This study was conducted to select the optimized temperature for preserving the quality-related traits and antioxidant potential of cornelian cherry fruits after harvest. The fruits were treated with 1% chitosan and then kept under 0, 5, 10, and 21 °C for 21 days. The results revealed that fruits kept under lower than room temperature (21 °C) better retained antioxidant capacity and had higher levels of phenolics, flavonoids, and anthocyanins and even higher antioxidant enzyme activity, hence attaining prolonged postharvest life. Considering the quantity attributes and the shelf life, the temperature of choice was 0 °C. Zero temperature was also the best to keep the antioxidant capacity of cornelian cherry fruits. Overall, the results showed that low temperature and chitosan pretreatment provide an efficient method for maintaining the nutritional quality and antioxidant capacity of cornelian cherry fruits during storage time.
Thermo Condition Determines the Uptake of Autumn and Winter Applied Nitrogen and Subsequent Utilization in Spring Tea (Camellia sinensis L.)
Authors: Ma Lifeng , Jiang Shuangfeng , Deng Min , Lv Lize , Xu Ze , Ruan Jianyun
Published: December 2021

The effect of thermal condition on the uptake of autumn and winter applied N and its subsequent utilization in spring tea (Camellia sinensis) was investigated by applying 15N enriched urea as single or split applications between October and February in two commercial plantations at Xingyang of Henan province and Yongchuan of Chongqing with different thermal conditions. The proportion of N derived from 15N-labeled urea (Ndff%) in fibrous root and mature leaves 15 days after application at Xingyang and the Ndff% of mature leaves on the day of the first spring tea harvest at both sites were the highest in the single October application. The Ndff% of the following spring tea was also the highest in the single October application at both sites. The results showed that application of N fertilizer in October relative to other later months most significantly improves the accumulation of plant N reserves and consequently contributes more significantly to the early spring tea. Such timing effect was related to the thermal condition, i.e., the growing degree days (°C•d, T > 8 °C) between the dates of fertilization and harvest of young shoots, which represents the combining effect of the temperature and the residence time of N fertilizer in the soil.
Association between Laughter and Lifestyle Diseases after the Great East Japan Earthquake: The Fukushima Health Management Survey
Authors: Eguchi Eri , Ohira Tetsuya , Nakano Hironori , Hayashi Fumikazu , Okazaki Kanako , Harigane Mayumi , Funakubo Narumi , Takahashi Atsushi , Takase Kanae , Maeda Masaharu , Yasumura Seiji , Yabe Hirooki , Kamiya Kenji ,   on behalf of the Fukushima Health Management Survey Group
Published: December 2021

We investigated the association between the frequency of laughter and lifestyle diseases after the Great East Japan Earthquake. We included 41,432 participants aged 30–89 years in the Fukushima Health Management Survey in fiscal year 2012 and 2013. Gender-specific, age-adjusted and multivariable odds ratios of lifestyle diseases were calculated using logistic regressions stratified by evacuation status. Those who laugh every day had significantly lower multivariable odds ratios for hypertension (HT), diabetes mellitus (DM) and heart disease (HD) for men, and HT and dyslipidemia (DL) for women compared to those who do not, especially in male evacuees. The multivariable odds ratios (95% confidence intervals) of HT, DM and HD (non-evacuees vs. evacuees) for men were 1.00 (0.89–1.11) vs. 0.85 (0.74–0.96), 0.90 (0.77–1.05) vs. 0.77 (0.64–0.91) and 0.92 (0.76–1.11) vs. 0.79 (0.63–0.99), and HT and DL for women were 0.90 (0.81–1.00) vs. 0.88 (0.78–0.99) and 0.80 (0.70–0.92) vs. 0.72 (0.62–0.83), respectively. The daily frequency of laughter was associated with a lower prevalence of lifestyle disease, especially in evacuees.
The Epidemiology of Injuries in Adults in Nepal: Findings from a Hospital-Based Injury Surveillance Study
Authors: Bhatta Santosh , Magnus Dan , Mytton Julie , Joshi Elisha , Bhatta Sumiksha , Adhikari Dhruba , Manandhar Sunil Raja , Joshi Sunil Kumar
Published: December 2021

This study aimed to develop and evaluate a model of hospital-based injury surveillance and describe the epidemiology of injuries in adults. One-year prospective surveillance was conducted in two hospitals in Hetauda, Nepal. Data were collected electronically for patients presenting to emergency departments (EDs) with injuries between April 2019 and March 2020. To evaluate the model’s sustainability, clinical leaders, senior managers, data collectors, and study coordinators were interviewed. The total number of patients with injuries over one year was 10,154, representing 30.7% of all patients visiting the EDs. Of patients with injuries, 7458 (73.4%) were adults aged 18 years and over. Most injuries (6434, 86%) were unintentional, with smaller proportions due to assault (616, 8.2%) and self-harm (408, 5.5%). The median age of adult patients was 33 years (IQR 25–47). Males had twice the rate of ED presentation compared with females (40.4 vs. 20.9/1000). The most common causes were road traffic accidents (32.8%), falls (25.4%), and animal/insect related injuries (20.1%). Most injured patients were discharged after treatment (80%) with 9.1% admitted to hospital, 8.1% transferred to other hospitals, and 2.1% died. In Nepal, hospital-based injury surveillance is feasible, and rich injury data can be obtained by embedding data collectors in EDs.
Predictors of At-Home Death for Cancer Patients in Rural Clinics in Japan
Authors: Watanabe Jun , Teraura Hiroyuki , Komatsu Kenichi , Yamaguchi Hironori , Kotani Kazuhiko
Published: December 2021

Background: The prediction of at-home deaths has become an important topic in rural areas of Japan with an advanced aging society. However, there are no well-established predictors to explain how these factors influence intention. This study aims to investigate the possible predictors of at-home death for cancer patients in rural clinics in Japan. Methods: This is a nationwide cross-sectional survey. A self-administered questionnaire was sent to 493 rural clinics in Japan. The main outcome was the realization of at-home deaths for cancer patients. Results: Among the 264 clinics (54%) that responded to the survey, there were 194 clinics with the realization of at-home death. The use of a clinical pathway (adjusted odds ratio 4.19; 95% confidence interval 1.57–11.19) and the provision of organized palliative care (adjusted odds ratio 19.16; 95% confidence interval 7.56–48.52) were associated with the prediction of at-home death, irrespective of island geography or the number of doctors and nurses. Conclusions: Having a clinical pathway and systematizing palliative care could be important to determine the possibility of at-home deaths for cancer patients in rural clinics in Japan.
Why Re-Invent the Wheel? Social Network Approaches Can Be Used to Mitigate SARS-CoV-2 Related Disparities in Latinx Seasonal Farmworkers
Authors: Kanamori Mariano , Castaneda Daniel , Self Kyle J. , Sanchez Lucy , Rosas Yesenia , Rodriguez Edda , Shrader Cho-Hee , Arroyo-Flores Juan , Johnson Ariana , Skvoretz John , Gomez Daniel , Williams Mark
Published: December 2021

Latinx seasonal farmworkers are essential workers and are at elevated risk for SARS-CoV-2 in the United States. Risk factors for SARS-CoV-2 are unique to this population and include crowded living conditions, isolated social networks, and exploitative working environments. The circumstances and cultural values of Latinx seasonal farmworkers pose a unique challenge to public health authorities working to contain the spread of SARS-CoV-2. This community is in dire need of urgent public health research to identify opportunities to prevent SARS-CoV-2 transmission: social network methods could be the solution. Using previously collected and new information provided by a team of experts, this commentary provides a brief description of Latinx seasonal farmworker disparities that affect tracking and treating SARS-CoV-2 in this important group, the challenges introduced by SARS-CoV-2, and how social network approaches learned from other infectious disease prevention strategies can address these disparities.
Reply to Ivy et al. Comment on “Khairul Zaman et al. Eco-Friendly Coagulant versus Industrially Used Coagulants: Identification of Their Coagulation Performance, Mechanism and Optimization in Water Treatment Process. Int. J. Environ. Res. Public Health 2021, 18, 9164”
Authors: Khairul Zaman Nadiah , Rohani Rosiah , Izni Yusoff Izzati , Kamsol Muhammad Azraei , Basiron Siti Aishah , Abd. Rashid Aina Izzati
Published: December 2021

We appreciate very much the interest of Ivy et al. in our work [...]
A Successful Dental Care Referral Program for Low-Income Pregnant Women in New York
Authors: Russell Stefanie L. , Kerpen Steven J. , Rabin Jill M. , Burakoff Ronald P. , Yang Chengwu , Huang Shulamite S.
Published: December 2021

Despite evidence-based guidelines that advocate for dental care during pregnancy, dental utilization among pregnant women remains low, especially among low-income and racial–ethnic minority women. We investigated self-reported dental care referral and self-reported dental care attendance among a group of 298 low-income, largely racial–ethnic minority pregnant women attending two suburban prenatal care clinics that had integrated dental care referrals into their prenatal care according to these guidelines. We administered a questionnaire that asked women: (1) whether they had been referred for care by their prenatal care provider; (2) whether they had been seen by a dentist during pregnancy. Among those women who were eligible for a dental care referral (those who reported having dental symptoms, and those not having a recent dental visit), we found that 73.0% reported that they had indeed been referred for dental care by their prenatal provider, while the remaining women reported either no referral (23.5%, n = 67) or were not sure whether they had been referred (3.5%, n = 10). Among those who reported a dental care referral, 67.3% (n = 140) reported that they saw a dentist during their pregnancy, while of those who reported no dental care referral only 35.1% (n = 27) reported a dental visit (Chi-Sq. = 24.1, df = 1, p < 0.001). Having received a dental referral was a significant predictor of reporting a dental visit during pregnancy, with women who received a referral being 4.6 times more likely to report a dental visit during pregnancy compared to those women who did not report a referral. These results demonstrate that vulnerable pregnant women referred for dental care by their prenatal provider will indeed seek and utilize dental care when offered. This dental referral program may serve as a model for improving the utilization of dental care among this population.
Epigenetic Modifications at the Center of the Barker Hypothesis and Their Transgenerational Implications
Authors: Ryznar Rebecca Jean , Phibbs Lacie , Van Winkle Lon J.
Published: December 2021

Embryo/fetal nutrition and the environment in the reproductive tract influence the subsequent risk of developing adult diseases and disorders, as formulated in the Barker hypothesis. Metabolic syndrome, obesity, heart disease, and hypertension in adulthood have all been linked to unwanted epigenetic programing in embryos and fetuses. Multiple studies support the conclusion that environmental challenges, such as a maternal low-protein diet, can change one-carbon amino acid metabolism and, thus, alter histone and DNA epigenetic modifications. Since histones influence gene expression and the program of embryo development, these epigenetic changes likely contribute to the risk of adult disease onset not just in the directly affected offspring, but for multiple generations to come. In this paper, we hypothesize that the effects of parental nutritional status on fetal epigenetic programming are transgenerational and warrant further investigation. Numerous studies supporting this hypothesis are reviewed, and potential research techniques to study these transgenerational epigenetic effects are offered.
Behavior Strategy Analysis Based on the Multi-Stakeholder Game under the Plastic Straw Ban in China
Authors: Chen Tinggui , Zhang Yuling , Yang Jianjun , Cong Guodong , Jiang Guozhang , Li Gongfa
Published: December 2021

Since 1 January 2021, China has banned nondegradable disposable straws in the catering industry. To promote the enforcement of the ban of plastic straws and improve the relationship between economic development and environmental protection, based on the evolutionary game method, this paper constructs the game model from the supply side and the demand side, respectively. Subsequently, through the dynamic equation, stable system evolution strategy is obtained. Furthermore, simulation is conducted to test the influence of the main parameters in the model on the evolution of system strategy. The results show that (1) the change of the government strategy mainly depends on its regulation costs and revenue, while the production strategy of a company is affected by the government and consumer strategies. (2) From the perspective of enterprise supply, government subsidies can promote technological innovation and develop new plastic straw substitutes. However, government penalties have little effect on violating enterprises. In addition, from the perspective of enterprise demand, with the collaboration of enterprises and consumers, it is easier for enterprises to carry out technological innovation. (3) Consumer acceptance of the substitutes for disposable plastic straws as well as online comments have a decisive influence on the enterprises’ selections for research and development (R&D) strategies.
An Inquiry into the Relationship between Drug Users’ Psychological Situations and Their Drug-Taking Behaviour
Authors: Lee Gabriel Kwun Wa , Chan Gloria , Lo T. Wing , Yeung Jerf W. K. , Tam Cherry H. L. , Guan Xin
Published: December 2021

In view of the research gap whereby few studies have investigated the inner psychological situations underlying continuous drug use, this study used the Soulmate Scale to investigate the relationship between soulmate experience and drug-taking behaviour. Overall, 276 participants took part in this study. Results showed that soulmate experience was negatively related to drug-taking behaviour, which means that being psychologically attached to drugs and receiving comfort from them encourages dependency and a higher level of difficulty in quitting drugs. In addition, soulmate experience significantly mediated the effect of meaning of life and social isolation on drugs, suggesting that when such psychological bonding and sustenance can be developed in interpersonal relationships instead of drugs, drug users are likely to develop the meaning of life and a lower sense of social isolation, and are more likely to quit drugs. The corresponding implications were discussed.
Structural Relationships among Strategic Experiential Modules, Motivation, Serious Leisure, Satisfaction and Quality of Life in Bicycle Tourism
Authors: Guo Rui , Liu Xiaoying , Song Hakjun
Published: December 2021

It seems that people’s quality of life can be positively influenced through bicycle tourism. Bicycle tourism can be an effective measure to enhance serious leisure, tourism satisfaction, and quality of life. To verify this empirically, a survey was conducted of bicycle tourists who visited Qinghai Lake in China during an international road bike race. The purpose of the present research is to prove the association between latent variables related to bicycle tourism through statistical analysis. For this, hypothetical relationships based on tourism motivation, serious leisure, tourism satisfaction, and quality of life were presented as research models. As a result of empirical analysis, it was analyzed that friends and nature had an effect on serious leisure among the motivation of bicycle tourism. In addition, it was found that the level of serious leisure for bicycle tourism exerted a positive influence on the satisfaction and quality of life. This suggests that bicycle tourism can improve the quality of life during travel to Qinghai lake by bicycle and revealed the crucial role in relationships is serious leisure.
BaGdF5 Nanophosphors Doped with Different Concentrations of Eu3+ for Application in X-ray Photodynamic Therapy
Authors: Gadzhimagomedova Zaira , Polyakov Vladimir , Pankin Ilia , Butova Vera , Kirsanova Daria , Soldatov Mikhail , Khodakova Darya , Goncharova Anna , Mukhanova Elizaveta , Belanova Anna , Maksimov Aleksey , Soldatov Alexander
Published: December 2021

X-ray photodynamic therapy (XPDT) has been recently considered as an efficient alternative to conventional radiotherapy of malignant tissues. Nanocomposites for XPDT typically consist of two components—a nanophosphor which re-emits X-rays into visible light that in turn is absorbed by the second component, a photosensitizer, for further generation of reactive oxygen species. In this study, BaGdF5 nanophosphors doped with different Eu:Gd ratios in the range from 0.01 to 0.50 were synthesized by the microwave route. According to transmission electron microscopy (TEM), the average size of nanophosphors was ~12 nm. Furthermore, different coatings with amorphous SiO2 and citrates were systematically studied. Micro-CT imaging demonstrated superior X-ray attenuation and sufficient contrast in the liver and the spleen after intravenous injection of citric acid-coated nanoparticles. In case of the SiO2 surface, post-treatment core–shell morphology was verified via TEM and the possibility of tunable shell size was reported. Nitrogen adsorption/desorption analysis revealed mesoporous SiO2 formation characterized by the slit-shaped type of pores that should be accessible for methylene blue photosensitizer molecules. It was shown that SiO2 coating subsequently facilitates methylene blue conjugation and results in the formation of the BaGdF5: 10% Eu3+@SiO2@MB nanocomposite as a promising candidate for application in XPDT.
Genome-Wide Analysis of WRKY Gene Family and the Dynamic Responses of Key WRKY Genes Involved in Ostrinia furnacalis Attack in Zea mays
Authors: Tang Yin , Guo Jingfei , Zhang Tiantao , Bai Shuxiong , He Kanglai , Wang Zhenying
Published: December 2021

WRKY transcription factors comprise one of the largest gene families and serve as key regulators of plant defenses against herbivore attack. However, studies related to the roles of WRKY genes in response to herbivory are limited in maize. In this study, a total of 128 putative maize WRKY genes (ZmWRKYs) were identified from the new maize genome (v4). These genes were divided into seven subgroups (groups I, IIa–e, and III) based on phylogenomic analysis, with distinct motif compositions in each subgroup. Syntenic analysis revealed that 72 (56.3%) of the genes were derived from either segmental or tandem duplication events (69 and 3, respectively), suggesting a pivotal role of segmental duplication in the expansion of the ZmWRKY family. Importantly, transcriptional regulation prediction showed that six key WRKY genes contribute to four major defense-related pathways: L-phenylalanine biosynthesis II and flavonoid, benzoxazinoid, and jasmonic acid (JA) biosynthesis. These key WRKY genes were strongly induced in commercial maize (Jingke968) infested with the Asian corn borer, Ostrinia furnacalis, for 0, 2, 4, 12 and 24 h in the field, and their expression levels were highly correlated with predicted target genes, suggesting that these genes have important functions in the response to O. furnacalis. Our results provide a comprehensive understanding of the WRKY gene family based on the new assembly of the maize genome and lay the foundation for further studies into functional characteristics of ZmWRKY genes in commercial maize defenses against O. furnacalis in the field.
Calmodulin-Connexin Partnership in Gap Junction Channel Regulation-Calmodulin-Cork Gating Model
Authors: Peracchia Camillo , Leverone Peracchia Lillian Mae
Published: December 2021

In the past four decades numerous findings have indicated that gap junction channel gating is mediated by intracellular calcium concentrations ([Ca2+i]) in the high nanomolar range via calmodulin (CaM). We have proposed a CaM-based gating model based on evidence for a direct CaM role in gating. This model is based on the following: CaM inhibitors and the inhibition of CaM expression to prevent chemical gating. A CaM mutant with higher Ca2+ sensitivity greatly increases gating sensitivity. CaM co-localizes with connexins. Connexins have high-affinity CaM-binding sites. Connexin mutants paired to wild type connexins have a higher gating sensitivity, which is eliminated by the inhibition of CaM expression. Repeated trans-junctional voltage (Vj) pulses progressively close channels by the chemical/slow gate (CaM’s N-lobe). At the single channel level, the gate closes and opens slowly with on-off fluctuations. Internally perfused crayfish axons lose gating competency but recover it by the addition of Ca-CaM to the internal perfusion solution. X-ray diffraction data demonstrate that isolated gap junctions are gated at the cytoplasmic end by a particle of the size of a CaM lobe. We have proposed two types of CaM-driven gating: “Ca-CaM-Cork” and “CaM-Cork”. In the first, the gating involves Ca2+-induced CaM activation. In the second, the gating occurs without a [Ca2+]i rise.
Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D
Authors: Mironenka Julia , Różalska Sylwia , Bernat Przemysław
Published: December 2021

Wheat is a critically important crop. The application of fungi, such as Trichoderma harzianum, to protect and improve crop yields could become an alternative solution to synthetic chemicals. However, the interaction between the fungus and wheat in the presence of stress factors at the molecular level has not been fully elucidated. In the present work, we exposed germinating seeds of wheat (Triticum aestivum) to the plant pathogen Fusarium culmorum and the popular herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of T. harzianum or its extracellular metabolites. Then, the harvested roots and shoots were analyzed using spectrometry, 2D-PAGE, and MALDI-TOF/MS techniques. Although F. culmorum and 2,4-D were found to disturb seed germination and the chlorophyll content, T. harzianum partly alleviated these negative effects and reduced the synthesis of zearalenone by F. culmorum. Moreover, T. harzianum decreased the activity of oxidoreduction enzymes (CAT and SOD) and the contents of the oxylipins 9-Hode, 13-Hode, and 13-Hotre induced by stress factors. Under the influence of various growth conditions, changes were observed in over 40 proteins from the wheat roots. Higher volumes of proteins and enzymes performing oxidoreductive functions, such as catalase, ascorbate peroxidase, cytochrome C peroxidase, and Cu/Zn superoxide dismutase, were found in the Fusarium-inoculated and 2,4-D-treated wheat roots. Additionally, observation of the level of 12-oxo-phytodienoic acid reductase involved in the oxylipin signaling pathway in wheat showed an increase. Trichoderma and its metabolites present in the system leveled out the mentioned proteins to the control volumes. Among the 30 proteins examined in the shoots, the expression of the proteins involved in photosynthesis and oxidative stress response was found to be induced in the presence of the herbicide and the pathogen. In summary, these proteomic and metabolomic studies confirmed that the presence of T. harzianum results in the alleviation of oxidative stress in wheat induced by 2,4-D or F. culmorum.
Neuroprotective Properties of Quinone Reductase 2 Inhibitor M-11, a 2-Mercaptobenzimidazole Derivative
Authors: Voronin Mikhail V. , Kadnikov Ilya A. , Zainullina Liana F. , Logvinov Ilya O. , Verbovaya Ekaterina R. , Antipova Tatyana A. , Vakhitova Yulia V. , Seredenin Sergei B.
Published: December 2021

The ability of NQO2 to increase the production of free radicals under enhanced generation of quinone derivatives of catecholamines is considered to be a component of neurodegenerative disease pathogenesis. The present study aimed to investigate the neuroprotective mechanisms of original NQO2 inhibitor M-11 (2-[2-(3-oxomorpholin-4-il)-ethylthio]-5-ethoxybenzimidazole hydrochloride) in a cellular damage model using NQO2 endogenous substrate adrenochrome (125 µM) and co-substrate BNAH (100 µM). The effects of M-11 (10–100 µM) on the reactive oxygen species (ROS) generation, apoptosis and lesion of nuclear DNA were evaluated using flow cytometry and single-cell gel electrophoresis assay (comet assay). Results were compared with S29434, the reference inhibitor of NQO2. It was found that treatment of HT-22 cells with M-11 results in a decline of ROS production triggered by incubation of cells with NQO2 substrate and co-substrate. Pre-incubation of HT-22 cells with compounds M-11 or S29434 results in a decrease of DNA damage and late apoptotic cell percentage reduction. The obtained results provide a rationale for further development of the M-11 compound as a potential neuroprotective agent.
Evaluating the Effect of Lenvatinib on Sorafenib-Resistant Hepatocellular Carcinoma Cells
Authors: Shi Tingting , Iwama Hisakazu , Fujita Koji , Kobara Hideki , Nishiyama Noriko , Fujihara Shintaro , Goda Yasuhiro , Yoneyama Hirohito , Morishita Asahiro , Tani Joji , Yamada Mari , Nakahara Mai , Takuma Kei , Masaki Tsutomu
Published: December 2021

Hepatocellular carcinoma (HCC) is one of the major causes of cancer-related deaths worldwide. Sorafenib has been used as a first-line systemic treatment for over a decade. However, resistance to sorafenib limits patient response and presents a major hurdle during HCC treatment. Lenvatinib has been approved as a first-line systemic treatment for advanced HCC and is the first agent to achieve non-inferiority against sorafenib. Therefore, in the present study, we evaluated the inhibition efficacy of lenvatinib in sorafenib-resistant HCC cells. Only a few studies have been conducted on this topic. Two human HCC cell lines, Huh-7 and Hep-3B, were used to establish sorafenib resistance, and in vitro and in vivo studies were employed. Lenvatinib suppressed sorafenib-resistant HCC cell proliferation mainly by inducing G1 cell cycle arrest through ERK signaling. Hep-3B sorafenib-resistant cells showed partial cross-resistance to lenvatinib, possibly due to the contribution of poor autophagic responsiveness. Overall, the findings suggest that the underlying mechanism of lenvatinib in overcoming sorafenib resistance in HCC involves FGFR4-ERK signaling. Lenvatinib may be a suitable second-line therapy for unresectable HCC patients who have developed sorafenib resistance and express FGFR4.
Study of the Cone-Shaped Drogue for a Deep-Towed Multi-Channel Seismic Survey System Based on Data-Driven Simulations
Authors: Zhu Xiangqian , Sun Mingqi , He Tianhao , Yu Kaiben , Zong Le , Choi Jin-Hwan
Published: December 2021

A drogue is used to stabilise and straighten seismic arrays so that seismic waves can be well-received. To embed the effect of a cone-shaped drogue into the numerical modelling of the deep-towed seismic survey system, one surrogate model that maps the relationship between the hydrodynamic characteristics of the drogue and towing conditions was obtained based on data-driven simulations. The sample data were obtained by co-simulation of the commercial software RecurDyn and Particleworks, and the modelling parameters were verified by physical experiments. According to the Morison formula, the rotational angle, angular velocity, angular acceleration, towing speed, and towing acceleration of the drogue were selected as the design variables and drag forces and aligning torque were selected as the research objectives. The sample data of more than 8500 sets were obtained from virtual manoeuvres. Subsequently, both polynomial and neural network regression algorithms were used to study these data. Finally, analysis results show that the surrogate model obtained by machine learning has good performance in predicting research objectives. The results also reveal that the neural network regression algorithm is superior to the polynomial regression algorithm, its largest error of mean square is less than 0.8 (N2/N2 mm2), and its R-squared is close to 1.
Is It Possible to Differentiate Pneumocystis jirovecii Pneumonia and Colonization in the Immunocompromised Patients with Pneumonia?
Authors: Aguilar Yudy A. , Rueda Zulma Vanessa , Maya María Angélica , Vera Cristian , Rodiño Jenniffer , Muskus Carlos , Vélez Lázaro A.
Published: December 2021

Respiratory sample staining is a standard tool used to diagnose Pneumocystis jirovecii pneumonia (PjP). Although molecular tests are more sensitive, their interpretation can be difficult due to the potential of colonization. We aimed to validate a Pneumocystis jirovecii (Pj) real-time PCR (qPCR) assay in bronchoscopic bronchoalveolar lavage (BAL) and oropharyngeal washes (OW). We included 158 immunosuppressed patients with pneumonia, 35 lung cancer patients who underwent BAL, and 20 healthy individuals. We used a SYBR green qPCR assay to look for a 103 bp fragment of the Pj mtLSU rRNA gene in BAL and OW. We calculated the qPCR cut-off as well as the analytical and diagnostic characteristics. The qPCR was positive in 67.8% of BAL samples from the immunocompromised patients. The established cut-off for discriminating between disease and colonization was Ct 24.53 for BAL samples. In the immunosuppressed group, qPCR detected all 25 microscopy-positive PjP cases, plus three additional cases. Pj colonization in the immunocompromised group was 66.2%, while in the cancer group, colonization rates were 48%. qPCR was ineffective at diagnosing PjP in the OW samples. This new qPCR allowed for reliable diagnosis of PjP, and differentiation between PjP disease and colonization in BAL of immunocompromised patients with pneumonia.
Effects of COVID-19 on Variations of Taxpayers in Tourism-Reliant Regions: The Case of the Mexican Caribbean
Authors: Cruz-Milan Oliver , Lagunas-Puls Sergio
Published: December 2021

Given the tourism industry’s risk and vulnerability to pandemics and the need to better understand the impacts on tourism destinations, this research assesses the effect of the COVID-19 outbreak on the variation of taxpayer units in the Mexican Caribbean region, which includes some of the major sun-and-sand beach destinations in Latin America. Using monthly data of registered taxpayer entities at the state and national levels as the analysis variable, probability distributions and definite integrals are employed to determine variations of the year following the lockdown, compared with previous years’ data. Results indicate that despite the government’s measures to restrict businesses’ operations and a reduction in tourism activities, registered taxpayers at the regional level did not decrease for most of 2020. Further, as business activities and tourism recovered, taxpayer units increased at the end of 2020 and beginning of 2021. Surprisingly, such a pattern was not observed at the national level, which yielded no statistically significant variations. A discussion of factors influencing the resilience of the tourism region in the study (e.g., outbound markets’ geographic proximity, absence of travel restrictions, closure of competing destinations) and implications for public finances are presented.
Efficiency and Determinants of Capital Structure in the Greek Pharmaceutical, Cosmetic and Detergent Industries
Authors: Tsolas Ioannis E.
Published: December 2021

The purpose of this paper is to investigate the relationship between a firm’s capital structure (i.e., leverage) and its operating environment, taking into account firm (i.e., efficiency, asset structure, profitability, size, age and risk) and industry effects. For a sample of Greek pharmaceutical, cosmetic and detergent (PCD) enterprises, firm efficiency was estimated using bootstrapped data envelopment analysis (DEA), and a leverage model was produced using ordinary least squares (OLS) regression. The findings confirm the significance of firm efficiency (i.e., the franchise-value hypothesis over the efficiency-risk hypothesis) and asset structure on leverage. Efficiency and overall and short-term leverage have a significant negative relationship, indicating that more efficient firms tend to choose a relatively low debt ratio. Pharma firms are more affected since they are less efficient than cosmetics and detergents firms. Furthermore, asset structure and short- and long- term leverage have a significant negative and positive relationship, respectively, indicating that the firms with more tangible assets have less short-term debt and more long-term debt in their capital structure. Cosmetic and detergent firms, which have slightly more tangible assets than pharma firms, appear to be able to substitute high-cost, short-term debt with the low-cost, long-term debt by using such assets as collateral.
Strength and Toughness of Waste Fishing Net Fiber-Reinforced Concrete
Authors: Nguyen Tri N. M. , Han Taek Hee , Park Jun Kil , Kim Jung J.
Published: December 2021

In this study, we estimate the potential efficiency of waste fishing net (WFN) fibers as concrete reinforcements. Three WFN fiber concentrations (1, 2, and 3% by volume) were mixed with concrete. Compressive strength, toughness, splitting tensile strength, and biaxial flexural tests were conducted. Compressive strength decreased but other properties increased as a function of fiber proportions. According to the mechanical strength observations and the ductility number, WFN fibers yielded benefits in crack arresting that improved the postcracking behavior and transformed concrete from a brittle into a quasi-brittle material. It is inferred that WFN fiber is a recycled and eco-friendly material that can be utilized as potential concrete reinforcement.
Microdome-Tunable Graphene/Carbon Nanotubes Pressure Sensors Based on Polystyrene Array for Wearable Electronics
Authors: Su Xingjie , Luo Chunli , Yan Weiguo , Jiao Junyi , Zhong Dongzhou
Published: December 2021

Resistive pressure sensors are appealing due to having several advantages, such as simple reading mechanisms, simple construction, and quick dynamic response. Achieving a constantly changeable microstructure of sensing materials is critical for the flexible pressure sensor and remains a difficulty. Herein, a flexible, tunable resistive pressure sensors is developed via simple, low-cost microsphere self-assembly and graphene/carbon nanotubes (CNTs) solution drop coating. The sensor uses polystyrene (PS) microspheres to construct an interlocked dome microstructure with graphene/CNTs as a conductive filler. The results indicate that the interlocked microdome-type pressure sensor has better sensitivity than the single microdome-type and single planar-type without surface microstructure. The pressure sensor’s sensitivity can be adjusted by varying the diameter of PS microspheres. In addition, the resistance of the sensor is also tunable by adjusting the number of graphene/CNT conductive coating layers. The developed flexible pressure sensor effectively detected human finger bending, demonstrating tremendous potential in human motion monitoring.
Characterization of the Flux System: Lithium-Aluminum Silicate (Li)–Alkali Feldspars (Na,K); Magnesium (Mg) and Calcium (Ca)–Silicates
Authors: Stempkowska Agata
Published: December 2021

In this paper, the system of natural mineral alkali fluxes used in typical mineral industry technologies was analyzed. The main objective was to lower the melting temperature of the flux systems. The research has shown that the best melting parameters in the Ca–Mg– (Li,Na,K) system were characterized by the composition: A-eutectic 20% and wollastonite 80%, and it was reached at temperature 1140 °C; in addition, this set had the widest melting interval. Selected thermal parameters of mineral flux systems were also calculated. The technological properties of mineral composites such as shrinkage and brightness were also analyzed.
Synthesis of Lignosulfonate-Based Dispersants for Application in Concrete Formulations
Authors: Magina Sandra , Barros-Timmons Ana , Evtuguin Dmitry V.
Published: December 2021

Lignosulfonates (LS) are products from the sulfite pulping process that could be applied as renewable environmentally-friendly polymeric surfactants. Being widely used as plasticizers and water-reducing admixtures in concrete formulations LS compete in the market with petroleum-based superplasticizers, such as naphthalene sulfonate formaldehyde polycondensate (NSF) and copolymer polycarboxylate ethers (PCE). In this work, different chemical modification strategies were used to improve LS performance as dispersants for concrete formulations. One strategy consisted in increasing the molecular weight of LS through different approaches, such as laccase and polyoxometalate-mediated polymerization, glyoxalation, and reversible addition-fragmentation chain transfer (RAFT) polymerization. The other strategy consisted of preparing LS-based non-ionic polymeric dispersants using two different epoxidized oligomer derivatives of poly(ethylene glycol) (PEG) and poly(propylene glycol) (PPG). Modified LS were used to prepare cement pastes, which were examined for their fluidity. Results revealed that the most promising products are PPG-modified LS due to the introduction of PPG chains by reaction with phenolic moieties in LS. The enhanced dispersant efficiency of the ensuing products is probably related not only to electrostatic repulsion caused by the sulfonic ionizable groups in LS but also to steric hindrance phenomena due to the grafted bulky PPG chains.
Evaluation of New Hollow Sleeve Composites for Direct Post-Core Construction
Authors: Yoshii Shinji , Garoushi Sufyan , Kitamura Chiaki , Vallittu Pekka K. , Lassila Lippo V.
Published: December 2021

The preset shape and diameter of a prefabricated FRC post rarely follows the anatomy of the root canal. To solve this problem, a new hollow sleeve composite (HSC) system for post-core construction was developed and characterized. A woven fiber was impregnated with two types of resins: Bis-GMA or PMMA, and rolled into cylinders with outer diameter of 2 mm and two different inner diameters, namely 1.2 or 1.5 mm. The commercial i-TFC system was used as a control. Dual-cure resin composite was injected into these sleeves. Additionally, conventional solid fiber post was used as the inner part of the sleeve. The three-point bending test was used to measure the mechanical properties of the specimens and the fracture surface was examined using an electron microscope (SEM). The HSC (1.5 mm, Bis-GMA) revealed a statistically similar flexural modulus but higher flexural strength (437 MPa) compared to i-TFC (239 MPa; ANOVA, p < 0.05). When a fiber post was added inside, all values had a tendency to increase. After hydrothermal accelerated aging, the majority of specimens showed a significant (p < 0.05) decrease in flexural strength and modulus. SEM fracture analysis confirmed that the delamination occurred at the interface between the outer and inner materials. The HSC system provided flexibility but still high mechanical values compared to the commercial system. Thus, this system might offer an alternative practical option for direct post-core construction.
A Full-Scale Experimental Investigation of Utility Poles Made of Glass Fibre Reinforced Polymer
Authors: Broniewicz Mirosław , Broniewicz Filip , Broniewicz Elżbieta
Published: December 2021

Utility poles made of glass fibre-reinforced polymer (GFRP) are becoming increasingly common in European countries. Therefore, it is necessary to accurately examine their structural properties to ensure the integrity and safety of the poles. The purpose of this article is to compare the bending resistance of GFRP composite lighting columns obtained using European standard procedures with full-scale experimental tests. Several composite lighting columns were tested as part of the research study, and coupon tests were performed to assess the material properties required to calculate their bending resistance according to European Standard (EN) 40-3-3. The results obtained differed significantly. Furthermore, it was observed that the current standard rules for obtaining the resistance of GFRP poles based on the limit state method show a higher load capacity of the column in comparison to the capacity obtained from the tests.
Investigation into the Integration of Impregnated Glass and Carbon Textiles in a Laboratory Mortar Extruder (LabMorTex)
Authors: Kalthoff Matthias , Raupach Michael , Matschei Thomas
Published: December 2021

A promising process for the automatization of concrete structures is extrusion or extrusion molding. An innovative approach is the extrusion of concrete with imbedded technical textiles as reinforcement. For a successful extrusion, the rheological properties of the fresh concrete have to be optimized, as it must be extrudable and have sufficient early strength after leaving the mouthpiece. Within the scope of this paper, a process was developed which allows the integration of flexible as well as stiff impregnated textiles into the extrusion process. For this purpose, different textile-reinforced mortars (TRM) were extruded and their material characteristics were investigated. The results show that the mortar cross-section is considerably strengthened, especially when using carbon textiles, and that extrusion has considerable potential to produce high-performance TRM composites. In uniaxial tension tests with TRM, as well as in the pure roving tensile strength tests, textile stresses of approx. 1200 MPa were achieved for the glass textile and approx. 2250 MPa for the carbon textile. The position of the textile layer deviated a maximal 0.4 mm from its predesigned position, which shows its potential for producing tailor-made TRM elements. In addition, by adjusting the mortar mix design, it was possible to reduce the global warming potential (GWP) of the extrusion compound by up to 49.3% compared to the initial composition from preliminary studies.