bluuebunny/crossref_metadata_2025 huggingface dataset:
Emergency Dispatch Approach for Power Systems with Hybrid Energy Considering Thermal Power Unit Ramping
Authors: Zhou Buxiang , Wu Jiale , Zang Tianlei , Cai Yating , Sun Binjie , Qiu Yiwei
Published: May 2023

Future power systems will face more extreme operating condition scenarios, and system emergency dispatch will face more severe challenges. The use of distributed control is a well-designed way to handle this. It enables multi-energy complementation by means of autonomous communication, which greatly improves the flexibility of the grid. First, in the context of global energy conservation and emission reduction, this paper adopts the energy usage method of “renewable energy is the main source of energy, supplemented by thermal power and energy storage” to reduce the system abandoned wind (light) rate while supplementing the energy storage capacity. Second, a consensus algorithm is added to the system while considering the coordination between thermal units and energy storage. An “interface” for autonomous communication between thermal units and energy storage is created using the incremental cost of each agent. To address the recurring issue of power imbalance during emergency dispatch of the system, the consensus algorithm is enhanced so that the communication interval varies with the unit rate. This is based on the climbing characteristics of each thermal power unit. Finally, the effectiveness of the proposed method is verified in an IEEE-30 bus system.
Effects of Hydrogen Addition on Premixed Combustion of Kerosene in SI Engine
Authors: Zhao Yuxuan , Wang Enhua , Shi Zhicheng
Published: May 2023

Spark ignition (SI) engines fueled with kerosene have broad application prospects in unmanned aviation vehicles. The knock phenomenon of kerosene in SI engines is a huge challenge, leading to a much lower power output than gasoline engines. In this context, the combustion characteristics of kerosene blending with hydrogen are analyzed numerically regarding the working conditions of an SI engine. First, the ignition delay time of a kerosene/hydrogen mixture is estimated for temperatures of 600–1000 K and pressures of 15–35 bar using the Tay mechanism. The effects of hydrogen addition are evaluated with a ratio of 0–0.4. The sensitivities of the main reactions that affect the ignition delay time are discussed. Then, the laminar flame speed is predicted using the HYCHEM-SK mechanism, and the effects of hydrogen addition on the net reaction rates of the main reactions are analyzed. The results indicate that the ignition delay time is shortened and the laminar flame speed is increased as the hydrogen addition ratio rises. Meanwhile, the ignition delay time decreases except for the NTC range, and the laminar flame speed increases evidently as the temperature rises. In addition, the ignition delay time decreases obviously as the pressure increases with a temperature greater than 750 K. However, the laminar flame speed declines at 600 K and 800 K, while an opposite trend exhibits at 1000 K as the pressure rises. The laminar flame speed increases by 23.85–24.82%, while the ignition delay time only decreases by 4.02–3.59% at 1000 K as the hydrogen addition ratio rises from 0 to 0.4, which will be beneficial for knock suppression.
Taking Flow Characterization to New Heights by Fiber Bragg Gratings Array
Authors: Li Jinfeng
Published: May 2023

With global warming increasing at a faster rate in recent decades than at any other moment in recorded history, nuclear power, among a wide range of energy-efficient technologies, has been identified as the primary energy source in decarbonization for the improvement of security and efficiency [...]
Improved Methods for UHF Localization of Partial Discharge in Air-Insulated Substations
Authors: Rashwan Ahmed , Reid Alistair
Published: May 2023

A time-difference-of-arrival-based partial discharge (PD) location system that utilises prior knowledge of substation layout is presented. We propose a new time delay estimator that employs onset detection techniques and demonstrate, through experimental results, that it is at least 2.8 times more accurate than other conventional estimators. Using knowledge of a substation’s layout, we develop a minimum mean-square-error (MMSE)-based location estimator and an algorithm that optimises antenna placement to maximise location accuracy in regions occupied by high-voltage equipment. Simulation results show that a system that uses the proposed techniques is 5.9 times more accurate than a more conventional system in a high-noise environment.
Short-Term Load Forecasting of the Greek Power System Using a Dynamic Block-Diagonal Fuzzy Neural Network
Authors: Kandilogiannakis George , Mastorocostas Paris , Voulodimos Athanasios , Hilas Constantinos
Published: May 2023

A dynamic fuzzy neural network for short-term load forecasting of the Greek power system is proposed, and an hourly based prediction for the whole year is performed. A DBD-FELF (Dynamic Block-Diagonal Fuzzy Electric Load Forecaster) consists of fuzzy rules with consequent parts that are neural networks with internal recurrence. These networks have a hidden layer, which consists of pairs of neurons with feedback connections between them. The overall fuzzy model partitions the input space in partially overlapping fuzzy regions, where the recurrent neural networks of the respective rules operate. The partition of the input space and determination of the fuzzy rule base is performed via the use of the Fuzzy C-Means clustering algorithm, and the RENNCOM constrained optimization method is applied for consequent parameter tuning. The performance of DBD-FELF is tested via extensive experimental analysis, and the results are promising, since an average percentage error of 1.18% is attained, along with an average yearly absolute error of 76.2 MW. Moreover, DBD-FELF is compared with Deep Learning, fuzzy and neurofuzzy rivals, such that its particular attributes are highlighted.
The Role of Distributed Energy Resources and Associated Business Models in the Decentralised Energy Transition: A Review
Authors: Hargroves Karlson , James Benjamin , Lane Joshua , Newman Peter
Published: May 2023

The shift from large-scale centralised energy systems to smaller scale decentralised systems based on Distributed Energy Resources (DER) is likely to cause a sector-wide replacement of current electricity management practices and business models—creating a new energy paradigm. If handled well, such a transition will not be inherently disruptive; however, it can cause major disruption if long-held views and assumptions are not rapidly reconsidered and renewed, and new supporting structures are not swiftly put in place—hence, if disruption is experienced it will be due to a lack of strategic responses rather than the nature of the technology. This paper clarifies the nature of DERs and outlines key issues and opportunities associated with a range of associated service configurations and business models. The paper outlines key factors affecting the viability of such approaches and identifies leverage points for accelerating uptake. The paper concludes by considering how shifting landscape factors and related opportunities in the coming decades will shape the transition to a decentralised energy system. This paper contains findings from research performed at the Renewable, Affordable, Clean Energy Cooperative Research Centre (RACE CRC) in Australia.
Dynamic Investigation of a Coupled Parabolic Trough Collector–Phase Change Material Tank for Solar Cooling Process in Arid Climates
Authors: Ajbar Abdelhamid , Lamrani Bilal , Ali Emad
Published: May 2023

The use of solar energy for cooling processes is advantageous for reducing the energy consumption of conventional air-conditioning systems and protecting the environment. In the present work, a solar-powered cooling system with parabolic trough collectors (PTC) and a phase change material (PCM) tank is numerically investigated in the arid climates of Saudi Arabia. The system contains a 160-kW double-effect absorption chiller powered by solar-heated pressurized water as a heat transfer fluid (HTF) and a shell and tube PCM as a thermal battery. The novelty of this paper is to investigate the feasibility and the potential of using a PTC solar field coupled to a PCM tank for cooling purposes in arid climates. The numerical method is adopted in this work, and a dynamic model is developed based on the lumped approach; it is validated using data from the literature. The functioning of the coupled system is investigated in both sunshine hours (charging period) and off-sunshine hours (discharging period). The PTC area in this work varies from 200 m2 to 260 m2 and the cooling capacity of the chiller ranges from 120 kW to 200 kW. Obtained results showed that the 160-kW chiller is fully driven by the 240 m2-solar PTC during the charging period and about 23% of solar thermal energy is stored in the PCM tank. It was demonstrated that increasing the PTC area from 220 m2 to 260 m2 leads to a reduction in the PCM charging time by up to 45%. In addition, it was found that an increase in the cooling loads from 120 kW to 200 kW induces a decrease in the stored thermal energy in the PCM tank from 450 kWh to 45 kWh. During the discharging period, the PCM tank can continue the cooling process with a stable delivered cooling power of 160 kW and an HTF temperature between 118 °C and 150 °C. The PCM tank used in the studied absorption chiller leads to a reduction of up to 30% in cooling energy consumption during off-sunshine hours.
MARC-Net: Terrain Classification in Parallel Network Architectures Containing Multiple Attention Mechanisms and Multi-Scale Residual Cascades
Authors: Fan Xiangsuo , Li Xuyang , Yan Chuan , Fan Jinlong , Yu Ling , Wang Nayi , Chen Lin
Published: May 2023

To address the problem that traditional deep learning algorithms cannot fully utilize the correlation properties between spectral sequence information and the feature differences between different spectra, this paper proposes a parallel network architecture land-use classification based on a combined multi-head attention mechanism and multiscale residual cascade called MARC-Net. This parallel framework is firstly implemented by deeply mining the features generated by grouped spectral embedding for information among spectral features by adding a multi-head attention mechanism, which allows semantic features to have expressions from more subspaces while fully considering all spatial location interrelationships. Secondly, a multiscale residual cascade CNN (convolutional neural network) is designed to fully utilize the fused feature information at different scales, thereby improving the network’s ability to represent different levels of information. Lastly, the features obtained by the multi-head attention mechanism are fused with those obtained by the CNN, and the merged resultant features are downgraded through the fully connected layer to obtain the classification results and achieve pixel-level multispectral image classification. The findings show that the algorithm proposed in this paper has an aggregate precision of 97.22%, compared to that of the Vision Transformer (ViT) with 95.08%; its performance on the Sentinel-2 dataset shows a huge improvement. Moreover, this article mainly focuses on the change rate of forest land in the study area. The Forest land area was 125.1143 km2 in 2017, 105.6089 km2 in 2019, and 76.3699 km2 in 2021, with an increase of 15.59%, an decrease of 0.97%, and increase of 14.76% in 2017–2019, 2019–2021 and 2017–2021, respectively.
Effect of Botrytis cinerea Activity on Glycol Composition and Concentration in Wines
Authors: Antal Eszter , Kállay Miklós , Varga Zsuzsanna , Nyitrai-Sárdy Diána
Published: May 2023

The content of 2,3-butanediol ((R,R) and meso isomers) and 1,2-propanediol in grape berries and “liquid samples” (all non-berry extracts) from the Tokaj wine region of Hungary was investigated. Our aim was to find out how the activity of Botrytis cinerea influences the concentrations of these compounds compared with healthy grapes. Based on the measured concentrations, we can make a distinction between healthy berries and noble, rotted, so-called aszú berries. We also investigated if there is a difference between finished aszú wines and liquids intended for aszú production. We wanted to investigate the amount and distribution of the stereoisomers of 2,3-butanediol and their proportions. The results of the HS-SPME-GC-FID analysis of the samples showed significant differences in the 2,3-butanediol content between healthy and botrytised, aszú berries and between liquid samples for aszú production and aszú wines. In the berry samples, meso-2,3-butanediol could not be detected, whereas in the liquid samples, we found good amounts of this isomer. This may be due to the fact that the appearance of the meso form of 2,3-butanediol is a consequence of alcoholic fermentation. Significant differences were found between wines from healthy grapes and wines from botrytised grapes in terms of the levo-2,3-butanediol content, so that from an analytical point of view, a difference can be made between wines from healthy and botrytised grapes. No significant differences were found between berry and liquid samples in terms of 1,2-propanediol concentrations during our tests.
Impact of Commercial Inactive Yeast Derivatives on Antiradical Properties, Volatile and Sensorial Profiles of Grašac Wines
Authors: Stamenković Stojanović Sandra , Mančić Stojan , Cvetković Dragan , Malićanin Marko , Danilović Bojana , Karabegović Ivana
Published: May 2023

This study shows the impact of three different commercial inactive yeast derivatives (IYDs) (Opti Less™, Noblesse™, Optimum White™, Lallemand, Canada and Oenolees MP™ Lafort, USA) during the 6-month aging period on the volatile profile, sensory attributes and antiradical activity, including polyphenols and the total free sulfhydryl (-SH groups) content, of Grašac wines made in sequential fermentation with native Hanseniaspora uvarum S-2 and Saccharomyces cerevisiae QA23. The addition of IYDs helped in maintaining the constant values of antiradical activity during aging by increasing polyphenolic values and mitigating the decrease in -SH groups. HS-SPME-GC-MS analysis showed that esters were the major volatile compounds, with ethyl-acetate and 2-phenyl-ethyl-acetate being the most abundant among all the samples, followed by ethyl-dodecaonate, ethyl-decanoate and 3-methyl-butyl-octanoate, all of them contributing to fruity and floral aromas in wine. As the concentration of IYDs increased, a corresponding rise in the levels of certain volatiles, such as 2-methyl-1-propanol, phenyl-ethyl-alcohol and ethyl-octanoate, was observed. Sensory analysis showed that the addition of IYDs generally improved the taste and odor profile of the wine by reducing astringency and increasing fullness and complexity, regardless of the IYD type. The results demonstrated that different IYDs may have varying effects on wine, with each product having its specific purposes, providing the tools for winemakers to carefully regulate and obtain the desired sensory profile of the wine.
Addressing Clean Label Trends in Commercial Meat Processing: Strategies, Challenges and Insights from Consumer Perspectives
Authors: Inguglia Elena S. , Song Zuo , Kerry Joseph P. , O’Sullivan Maurice G. , Hamill Ruth M.
Published: May 2023

Background: The concept of a clean label is difficult to define, even in common language, as the interpretation of what a “clean” food is differs from one person to another and from one organisation to another. The lack of a unique definition and regulations of what the term “clean” means, along with the growing consumer demand for more “natural” and healthier foods, is posing new challenges for manufacturers and ingredient producers. The meat industry, in particular, has been affected by this new movement owing to negative attitudes and feelings consumers associate with consuming processed meat products. Scope and approach: The review scope is to describe attributes and associations around the “clean” label term by analysing the most recent ingredients, additives and processing methods currently available for meat manufacturers. Their application in meat, plant-based alternatives and hybrid meat/plant products, current limitations and challenges presented in consumer perception, safety and potential impacts on product quality are also presented. Key findings and conclusions: The availability of a growing number of “clean” label ingredients provides a new suite of approaches that are available for application by meat processors to help overcome some of the negative connotations associated with processed meat products and also support plant-based meat alternatives and hybrids.
Spatiotemporal Characteristics of Food Supply–Demand Balance in Uzbekistan under Different Scenarios
Authors: Song Xinzhe , Yang Yanzhao , Xiao Chiwei , Zhang Chao , Liu Ying , Wang Yuanqing
Published: May 2023

The food supply–demand balance is a perpetual concern for many countries, especially developing countries, such as Uzbekistan. Using the land resource carrying capacity model, here, food supply and demand for the cereals and calories in Uzbekistan during 1995–2020 were revealed. Despite increased demand for cereals and calories, unstable crop production has led to volatile growth patterns. The carrying capacity of cropland resources under Uzbekistan’s consumption standard shifted from overload to surplus and then to balance. Moreover, the carrying capacity of cropland resources under the healthy diet standard moved from balance to surplus in the past 25-years. Additionally, the calorific equivalent land resource carrying capacity under Uzbekistan’s consumption standard fluctuated, with the carrying state shifting from balance to surplus, and the healthy diet standard still in overload. These findings can help guide sustainable production and consumption strategies in Uzbekistan and other countries by analyzing the consumption structure and changes in supply and demand relationships.
Effect of 3D Food Printing Processing on Polyphenol System of Loaded Aronia melanocarpa and Post-Processing Evaluation of 3D Printing Products
Authors: Zhou Quancheng , Nan Xijun , Zhang Shucheng , Zhang Liang , Chen Jian , Li Jiayi , Wang Honglei , Ruan Zheng
Published: May 2023

Aronia melanocarpa polyphenols (AMP) have good nutritional values and functions. This study aimed to explore the printability and storage properties of AM gels in 3D food printing (3DFP). Therefore, 3DFP was performed on a loaded AMP gel system to determine its textural properties, rheological properties, microstructure, swelling degree and storage performance. The results revealed that the best loading AMP gel system to meet the printability requirements of 3DFP processing was AM fruit pulp:methylcellulose:pea albumin: hyaluronic acid = 100:14:1:1. Compared with other ratios and before 3DFP processing, the best loading AMP gel system processed by 3DFP exhibited the lowest deviation of 4.19%, the highest hardness, the highest elasticity, the least adhesion, a compact structure, uniform porosity, difficulty in collapsing, good support, a high degree of crosslinking, and good water retention. Additionally, they could be stored for 14 d at 4 °C. After post-processing, the AMP gel had a favorable AMP release rate and good sustained release effect in gastrointestinal digestion, which conformed to the Ritger–Peppas equation model. The results revealed that the gel system had good printability and applicability for 3D printing; as well, 3DFP products had good storage properties. These conclusions provide a theoretical basis for the application of 3D printing using fruit pulp as a raw material.
Yam Bean (Pachyrhizus erosus L. Urban) Powder Improves Grass Carp Myofibrillar Protein Gel by Forming Disulfide Bonds, Hydrogen Bonds, and Proper Microstructure
Authors: Song Geyao , Yang Kun , Wu Di , Lu Weiwei , Chi Rongshuo , Ma Jing , Sun Weiqing
Published: May 2023

This study aimed to analyze the impact of different additions (0–1.25%) of yam bean powder (YBP) on myofibrillar protein (MP) gel characteristics such as the structure, water-holding capacity (WHC), chemical interaction strength of grass carp MP, and texture. The results showed that the YBP exhibited a strong water absorption capacity and filled in the protein heat-induced polymerization gel network well, which enabled the gel network to capture and retain water effectively, resulting in MP gels containing YBP with excellent WHC and gel strength (0.75%). In addition, YBP induced the formation of hydrogen and disulfide bonds in proteins and inhibited the conversion of α-helices to β-sheets and β-turn structures, facilitating the formation of high-strength gel networks (p < 0.05). In conclusion, YBP can significantly improve the thermally induced gelling properties of grass carp MP. In particular, the addition of 0.75% YBP had the best effect in terms of filling the gel network of grass carp MP, resulting in the formation of a continuous and dense protein network, leading to the composite gel with the best WHC and texture.
Analysis of Cauchy Problems and Diffusion Equations Associated with the Hilfer–Prabhakar Fractional Derivative via Kharrat–Toma Transform
Authors: Dubey Ved Prakash , Singh Jagdev , Dubey Sarvesh , Kumar Devendra
Published: May 2023

In this paper, the Kharrat–Toma transforms of the Prabhakar integral, a Hilfer–Prabhakar (HP) fractional derivative, and the regularized version of the HP fractional derivative are derived. Moreover, we also compute the solution of some Cauchy problems and diffusion equations modeled with the HP fractional derivative via Kharrat–Toma transform. The solutions of Cauchy problems and the diffusion equations modeled with the HP fractional derivative are computed in the form of the generalized Mittag–Leffler function.
Copper, Uranium and REE Mineralisation in an Exhumed Oil Reservoir, Southwest Orkney, Scotland
Authors: Heptinstall Eleanor A. , Parnell John , Armstrong Joseph G. T. , Schito Andrea , Akinsanpe Temitope O.
Published: May 2023

Copper, uranium, and rare earth element (REE) mineralisation occurs in hydrocarbon-bearing Devonian continental sandstones in southwest Orkney, Scotland. The aeolian Yesnaby Sandstone Formation and fluvial Harra Ebb Sandstone Formation were mineralised following oil emplacement. The REE-bearing APS mineral florencite is particularly associated with bituminous nodules, many of which contain brannerite. Subsequently hydrothermal copper and other sulphides, and barite, further mineralised the oil reservoir at a temperature of ~190 °C. Oil was mobilised through mineralised fractures at this stage. Biodegradation of the oil occurred later, following the Carboniferous-Permian uplift. The occurrence confirms that Cu-APS mineralisation is possible in relatively low-temperature regimes in sedimentary basins.
Effectiveness of the Boston Brace in the Treatment of Paediatric Scoliosis: A Longitudinal Study from 2010–2020 in a National Spinal Centre
Authors: Tsirikos Athanasios I. , Adam Rachel , Sutters Kirsty , Fernandes Maureen , García-Martínez Silvia
Published: May 2023

Bracing can reduce curve progression in order to prevent or delay scoliosis surgery in growing children. Brace treatment is effective in adolescent idiopathic scoliosis (AIS), but there is less evidence of its efficacy in early-onset or non-idiopathic scoliosis. We assessed the outcome of bracing at the end point of treatment, including the patients’ perception of clinical results. We reviewed 480 patients treated using Boston brace from 2010–2020 (70% female); 249 patients completed bracing (52%) and 118 patients (47.4%) did not require surgery, with 83% having idiopathic scoliosis. Brace success was considered scoliosis below 50° at the end of bracing, with the patient skeletally mature. A total of 131 patients required scoliosis surgery after bracing (64% had idiopathic scoliosis; adolescents 57% and juveniles 43%). All patients had a minimum two-year follow-up after bracing or after scoliosis correction, with the quality of life assessment questionnaires. A total of 98 out of 182 patients with idiopathic scoliosis did not require surgery (54%). Thoracic scoliosis improved with bracing by a mean of 3.4° and thoracolumbar/lumbar scoliosis by a mean of 6.8°. A total of 85 patients with AIS (64%) but only 9 patients with JIS (20%) did not need surgery. In the AIS group, 97 patients had scoliosis of 20–40°; 71 of these patients (73.2%) did not require scoliosis correction at the end of bracing. In total, 84 patients with idiopathic scoliosis had surgery at a mean of 14 years (surgery was delayed by a mean of 3.2 years). In total, 20 of 67 patients with non-idiopathic scoliosis did not need surgery (30%). Thoracic scoliosis improved with bracing by a mean of 8.4° and thoracolumbar/lumbar scoliosis by a mean of 0.8°. A total of 47 patients with non-idiopathic scoliosis required surgery at a mean of 13.1 years (surgery was delayed by a mean of 5.2 years). Multivariate regression analysis showed that idiopathic scoliosis, AIS, closed triradiate cartilage, post-menarche status, higher Risser grade and smaller scoliosis angle at initial presentation predicted brace success. Patients reported good function and self-image, reduced pain and high satisfaction after treatment in both the bracing-only and the bracing followed by surgery groups.
Chronic Low Back Pain and Incident Transient Ischemic Attack and Stroke in General Practices in Germany
Authors: Jacob Louis , Smith Lee , Koyanagi Ai , Haro Josep Maria , Shin Jae Il , Tanislav Christian , Schnitzler Alexis , Kostev Karel
Published: May 2023

The aim was to investigate the association between chronic low back pain (CLBP) and incident transient ischemic attack (TIA) and stroke in Germany. The present retrospective cohort study included adults aged ≥18 years who were diagnosed for the first time with CLBP in one of 1198 general practices in Germany in 2005–2019 (index date). Patients without CLBP were matched to those with CLBP (1:1) using a propensity score based on age, sex, the index year, the number of medical consultations per year during the follow-up, and the number of years of follow-up. In patients without CLBP, the index date was a randomly selected visit date. Both groups were followed for up to 10 years. There were 159,440 patients included in the study (mean (SD) age: 52.1 (16.5) years; 51.5% women). Within 10 years of the index date, 6.5% and 5.9% of patients with and without CLBP were diagnosed with TIA or stroke, respectively (log-rank p-value < 0.001). The Cox regression analysis corroborated these results, as there was a significant association between CLBP and incident TIA or stroke (HR = 1.28, 95% CI = 1.22–1.35). CLBP was positively and significantly associated with incident TIA and stroke in Germany. More research is warranted to better understand this relationship.
Sex Differences in Maximal Oxygen Uptake Adjusted for Skeletal Muscle Mass in Amateur Endurance Athletes: A Cross Sectional Study
Authors: Martins Higgor Amadeus , Barbosa José Geraldo , Seffrin Aldo , Vivan Lavínia , Souza Vinicius Ribeiro dos Anjos , De Lira Claudio Andre Barbosa , Weiss Katja , Knechtle Beat , Andrade Marilia Santos
Published: May 2023

Male athletes tend to outperform female athletes in several endurance sports. Maximum cardiac output can be estimated by maximal oxygen consumption (V˙O2max), and it has been established that men present V˙O2max values about 20% higher than women. Although sex differences in V˙O2max have already been well studied, few studies have assessed sex differences with regard to muscle oxidative capacity. The aim of this study was to compare aerobic muscle quality, accessed by V˙O2max and adjusted by lower limb lean mass, between male and female amateur triathletes. The study also aimed to compare sex differences according to V˙O2 submaximal values assessed at ventilatory thresholds. A total of 57 participants (23 women and 34 men), who had been training for Olympic-distance triathlon races, underwent body composition evaluation by dual-energy X-ray absorptiometry and performed a cardiorespiratory maximal test on a treadmill. Male athletes had significantly higher V˙O2max, both absolutely and when adjusted to body mass. Conversely, when V˙O2max was adjusted for lean mass, there was no significant difference between sexes. The same was observed at submaximal exercise intensities. In conclusion, differences in V˙O2max adjusted to body mass but not lean mass may explain, at least in part, sex differences in performance in triathlons, marathons, cycling, and other endurance sports.
The Relationship between Nomophobia, Insomnia, Chronotype, Phone in Proximity, Screen Time, and Sleep Duration in Adults: A Mobile Phone App-Assisted Cross-Sectional Study
Authors: Jahrami Haitham
Published: May 2023

Earlier studies that have investigated the association between nomophobia and insomnia revealed that a strong relationship exists between both variables. This study aimed to explore possible associations between these variables and their impact on physical and mental health outcomes using a cross-sectional study design and mobile phone apps to collect data. Using a survey approach, data were collected from 444 participants (52% female, mean age 34 ± 12) using the Nomophobia Questionnaire (NMP-Q), the Insomnia Severity Index (ISI), the Morningness–Eveningness Questionnaire (MEQ) and three Android mobile phone apps. The Plees Tracker, screen time, and pedometer apps aided in collecting data on sleep duration, time spent on screen per day, and how close the phone was to the person. A statistically significant association was noted between nomophobia and insomnia, nomophobia and the eveningness chronotype, and nomophobia and screen time. The eveningness chronotype was also associated with an increased screen time use. The results show that NMP-Q, ISI, and screen time increase according to the chronotype. No statistically significant differences were noted in daily steps or sleep duration according to chronotype. The findings suggest that interventions targeting nomophobia may be beneficial in addressing insomnia among adults, particularly those with an evening chronotype. Future studies should consider exploring the causal relationship between them.
Quantification and Distribution of Primary and Secondary Metabolites in the Inner and Outer Parts of Strawberry Fruit
Authors: Simkova Kristyna , Veberic Robert , Hudina Metka , Cvelbar Weber Nika , Smrke Tina , Grohar Mariana Cecilia , Ivancic Tea , Pelacci Massimiliano , Medic Aljaz , Jakopic Jerneja
Published: May 2023

The distribution of primary and secondary metabolites within a fruit can affect its nutritional and organoleptic quality, as fruit can vary in size and shape. This study investigated the differences in the distribution of the primary and secondary metabolites in the fruit of four strawberry cultivars (‘Asia’, ‘Clery’, ‘Frederica’, and ‘Sandra’) that were collected at one harvest point. The study included an analysis of the individual sugars, organic acids, phenolic compounds, and enzymes responsible for the degradation of phenolics. All of the studied cultivars showed a lower pH, higher total organic acid content, and lower glucose and fructose content in the outer part of the fruit. Differences were also observed in the total phenolic and anthocyanin contents, which were always higher in the outer part. The absolute differences in the total phenolic content ranged from 3723 to 6154 mg kg−1 dry weight. Our results provide a basis for understanding the differences in the biosynthesis of these metabolites within this fruit and prove that it is essential to mix samples well before extractions to obtain results that are representative of the whole fruit.
Evaluation of the Nutritional, Phytochemical, and Antioxidant Potential of Rourea minor Fruits: An Underutilized Species
Authors: Murthy Hosakatte Niranjana , Yadav Guggalada Govardhana , Kadapatti Sathish Shekhappa , Pote Akarsha H. , Jagali Ramalinga , Yarashi Vidya , Dewir Yaser Hassan
Published: May 2023

The present study focuses on the nutritional, phytochemical composition, and antioxidant activities of the fruits of Rourea minor (Gaertn.) Alston., an underutilized plant species. The ripened seeds contained 0.28%, 12.39%, 25.70%, 1.4%, and 3.4% of fat, protein, carbohydrate, ash, and fiber, respectively, whereas ripened pulp possessed 0.19%, 0.34%, 0.90%, 0.35%, and 0.98% of fat, protein, carbohydrates, ash, and fiber, respectively. The ripened seed and pulp were also rich in mineral elements and especially microelements. The pulp, when ripe, had high levels of microelements such as boron, iron, zinc, copper, and manganese with values of 25.98, 2523.56, 499.12, 33.62, and 40.30 µg/g DW, respectively. Phytate and oxalate content were comparatively higher in ripened seeds (6.91 and 31.88 mg/g FW) than in the pulp. Acetone, absolute methanol, water, and 70% methanol were used for the extraction of phytochemicals, and 70% methanol extract contained the highest phytochemicals. The total phenolic, flavonoid, and alkaloid content of unripe seeds was 180.47 mg gallic acid equivalent (GAE)/DW, 68.95 quercetin equivalent (QE)/g DW, and 0.35 atropine equivalent (AE)/g DW, respectively, and that of unripe pulp was 8.21 mg GAE/g DW, 2.97 mg QE/g DW and 1.20 mg AE/g DW, respectively. All the extracts showed remarkable antioxidant activity, proved by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, total antioxidant activity, and ferric-reducing antioxidant power (FRAP) assay, following a similar trend to the phytochemical composition. The study concludes that Rourea minor fruits, both seeds, and pulp, could be an excellent source of nutrients, microelements, and antioxidants.
Impact of National Tobacco Control Policy on Rates of Hospital Admission for Pneumonia: When Compliance Matters
Authors: Gambaryan Marine , Kontsevaya Anna , Drapkina Oxana
Published: May 2023

A number of studies claim that tobacco control (TC) regulations are associated with reductions in smoking-related hospitalisation rates, but very few have estimated the impact of TC laws (TCL) at both countrywide and regional levels, and none of them have studied the impact of TCL in relation to compliance with TC regulations. This study evaluates the effects of Russian TCL on hospital admission (HA) rates for pneumonia countrywide and in 10 Russian regions and the extent of these effects in connection with the compliance with TCL. Methods: HA rates for pneumonia from 2005–2019 were analysed to compare the periods before and after the adoption of TCL in 2013. An interrupted time series design and a Poisson regression model were used to estimate the immediate and long-term effects of TCL on pneumonia annual hospitalisation rates after the TCL adoption, compared with the pre-law period. The 10 Russian regions were compared using the TCL implementation scale (TCIS) developed on the basis of the results of the Russian TC policy evaluation survey; Spearman’s rank correlation and linear regression models were employed. Results showed a 14.3% reduction in HA rates for pneumonia (RR 0.88; p = 0.01) after the adoption of TCL in Russia with significant long-term effect after 2013 (RR 0.86; p = 0.006). Regions with better enforcement of TCL exhibited greater reductions in pneumonia HA rates (rsp = −0.55; p = 0.04); (β = −4.21; p = 0.02). Conclusions: TCL resulted in a sustained reduction in pneumonia hospitalisation rates, but these effects, varying by region, may depend on the scale of the TCL enforcement.
Nanomaterials in Biomedicine 2022
Authors: Arcos Daniel
Published: May 2023

Nanomaterials in biomedicine are materials designed at a scale of 1–100 nanometers that make it possible to diagnose, treat and prevent diseases using tools and knowledge of the human body at the molecular scale [...]
Fighting Methicillin-Resistant Staphylococcus aureus with Targeted Nanoparticles
Authors: Andrade Stéphanie , Ramalho Maria J. , Santos Sílvio B. , Melo Luís D. R. , Santos Rita S. , Guimarães Nuno , Azevedo Nuno F. , Loureiro Joana A. , Pereira Maria C.
Published: May 2023

Antimicrobial resistance (AMR) is considered one of the greatest threats to global health. Methicillin-resistant Staphylococcus aureus (MRSA) remains at the core of this threat, accounting for about 90% of S. aureus infections widespread in the community and hospital settings. In recent years, the use of nanoparticles (NPs) has emerged as a promising strategy to treat MRSA infections. NPs can act directly as antibacterial agents via antibiotic-independent activity and/or serve as drug delivery systems (DDSs), releasing loaded antibiotics. Nonetheless, directing NPs to the infection site is fundamental for effective MRSA treatment so that highly concentrated therapeutic agents are delivered to the infection site while directly reducing the toxicity to healthy human cells. This leads to decreased AMR emergence and less disturbance of the individual’s healthy microbiota. Hence, this review compiles and discusses the scientific evidence related to targeted NPs developed for MRSA treatment.
Antimicrobial Peptides: Challenging Journey to the Pharmaceutical, Biomedical, and Cosmeceutical Use
Authors: Mazurkiewicz-Pisarek Anna , Baran Joanna , Ciach Tomasz
Published: May 2023

Antimicrobial peptides (AMPs), or host defence peptides, are short proteins in various life forms. Here we discuss AMPs, which may become a promising substitute or adjuvant in pharmaceutical, biomedical, and cosmeceutical uses. Their pharmacological potential has been investigated intensively, especially as antibacterial and antifungal drugs and as promising antiviral and anticancer agents. AMPs exhibit many properties, and some of these have attracted the attention of the cosmetic industry. AMPs are being developed as novel antibiotics to combat multidrug-resistant pathogens and as potential treatments for various diseases, including cancer, inflammatory disorders, and viral infections. In biomedicine, AMPs are being developed as wound-healing agents because they promote cell growth and tissue repair. The immunomodulatory effects of AMPs could be helpful in the treatment of autoimmune diseases. In the cosmeceutical industry, AMPs are being investigated as potential ingredients in skincare products due to their antioxidant properties (anti-ageing effects) and antibacterial activity, which allows the killing of bacteria that contribute to acne and other skin conditions. The promising benefits of AMPs make them a thrilling area of research, and studies are underway to overcome obstacles and fully harness their therapeutic potential. This review presents the structure, mechanisms of action, possible applications, production methods, and market for AMPs.
A Comparative Study on the Effects of Different Sources of Carboxymethyl Poria Polysaccharides on the Repair of DSS-Induced Colitis in Mice
Authors: Tan Zhijie , Zhang Qiaoyi , Zhao Rou , Huang Ting , Tian Yun , Lin Yuanshan
Published: May 2023

Carboxymethyl poria polysaccharide plays important anti-tumor, antioxidant, and anti-inflammatory roles. Therefore, this study aimed to compare the healing impacts of two different sources of carboxymethyl poria polysaccharides [Carboxymethylat Poria Polysaccharides I (CMP I) and Carboxymethylat Poria Polysaccharides II (CMP II)] on ulcerative colitis in mice caused by dextran sulfate sodium (DSS). All the mice were arbitrarily split into five groups (n = 6): (a) control (CTRL), (b) DSS, (c) SAZ (sulfasalazine), (d) CMP I, and (e) CMP II. The experiment lasted for 21 days, and the body weight and final colon length were monitored. A histological analysis of the mouse colon tissue was carried out using H&E staining to assess the degree of inflammatory infiltration. The levels of inflammatory cytokines [interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-4 (IL-4)] and enzymes [superoxide dismutase (SOD) and myeloperoxidase (MPO)] in the serum were examined using ELISA. Additionally, 16S ribosomal RNA sequencing was used to analyze the microorganisms in the colon. The results indicated that both CMP I and CMP II alleviated weight loss, colonic shortening, and inflammatory factor infestation in colonic tissues caused by DSS (p < 0.05). Furthermore, the ELISA results revealed that both CMP I and CMP II reduced the expression of IL-1β, IL-6, TNF-α, and MPO, and elevated the expression of IL-4 and SOD in the sera of the mice (p < 0.05). Moreover, 16S rRNA sequencing showed that CMP I and CMP II increased the plenitude of microorganisms in the mouse colon relative to that in the DSS group. The results also indicated that the therapeutic effect of CMP I on DSS-induced colitis in the mice was superior to that of CMP II. This study demonstrated that carboxymethyl poria polysaccharide from Poria cocos had therapeutic effects on DSS-induced colitis in mice, with CMP I being more effective than CMP II.
Mitochondrial Connexins and Mitochondrial Contact Sites with Gap Junction Structure
Authors: Cetin-Ferra Selma , Francis Sharon C. , Cooper Anthonya T. , Neikirk Kit , Marshall Andrea G. , Hinton Antentor , Murray Sandra A.
Published: May 2023

Mitochondria contain connexins, a family of proteins that is known to form gap junction channels. Connexins are synthesized in the endoplasmic reticulum and oligomerized in the Golgi to form hemichannels. Hemichannels from adjacent cells dock with one another to form gap junction channels that aggregate into plaques and allow cell–cell communication. Cell–cell communication was once thought to be the only function of connexins and their gap junction channels. In the mitochondria, however, connexins have been identified as monomers and assembled into hemichannels, thus questioning their role solely as cell–cell communication channels. Accordingly, mitochondrial connexins have been suggested to play critical roles in the regulation of mitochondrial functions, including potassium fluxes and respiration. However, while much is known about plasma membrane gap junction channel connexins, the presence and function of mitochondrial connexins remain poorly understood. In this review, the presence and role of mitochondrial connexins and mitochondrial/connexin-containing structure contact sites will be discussed. An understanding of the significance of mitochondrial connexins and their connexin contact sites is essential to our knowledge of connexins’ functions in normal and pathological conditions, and this information may aid in the development of therapeutic interventions in diseases linked to mitochondria.
Metformin and Canagliflozin Are Equally Renoprotective in Diabetic Kidney Disease but Have No Synergistic Effect
Authors: Corremans Raphaëlle , Vervaet Benjamin A. , Dams Geert , D’Haese Patrick C. , Verhulst Anja
Published: May 2023

Diabetic Kidney Disease (DKD) is a major microvascular complication for diabetic patients and is the most common cause of chronic kidney disease (CKD) and end-stage renal disease. Antidiabetic drugs, such as metformin and canagliflozin, have been shown to exert renoprotective effects. Additionally, quercetin recently showed promising results for the treatment of DKD. However, the molecular pathways through which these drugs exert their renoprotective effects remain partly unknown. The current study compares the renoprotective potential of metformin, canagliflozin, metformin + canagliflozin, and quercetin in a preclinical rat model of DKD. By combining streptozotocin (STZ) and nicotinamide (NAD) with daily oral N(ω)-Nitro-L-Arginine Methyl Ester (L-NAME) administration, DKD was induced in male Wistar Rats. After two weeks, rats were assigned to five treatment groups, receiving vehicle, metformin, canagliflozin, metformin + canagliflozin, or quercetin for a period of 12 weeks by daily oral gavage. Non-diabetic vehicle-treated control rats were also included in this study. All rats in which diabetes was induced developed hyperglycemia, hyperfiltration, proteinuria, hypertension, renal tubular injury and interstitial fibrosis, confirming DKD. Metformin and canagliflozin, alone or together, exerted similar renoprotective actions and similar reductions in tubular injury and collagen accumulation. Renoprotective actions of canagliflozin correlated with reduced hyperglycemia, while metformin was able to exert these effects even in the absence of proper glycemic control. Gene expression revealed that the renoprotective pathways may be traced back to the NF-κB pathway. No protective effect was seen with quercetin. In this experimental model of DKD, metformin and canagliflozin were able to protect the kidney against DKD progression, albeit in a non-synergistic way. These renoprotective effects may be attributable to the inhibition of the NF-κB pathway.
Achieving High Expression of Cry in Green Tissues and Negligible Expression in Endosperm Simultaneously via rbcS Gene Fusion Strategy in Rice
Authors: Chen Hao , Huang Yuqing , Ye Mengnan , Wang Ya , He Xiuying , Tu Jumin
Published: May 2023

To allay excessive public concern about the safety of transgenic foods, and to optimize insect-resistant genes expression to delay the evolution of resistance in pests, we developed a promising strategy to fuse the GOI (gene of interest) with OsrbcS (rice small subunit of ribulose bisphosphate carboxylase/oxygenase) in transgenic rice, which acted as a carrier, driven by the OsrbcS native promoter to sequester its expression in green tissues. Using eYFP as a trial, we reported a high-level accumulation of eYFP in green tissue and almost none in the seed and root of the fused construct compared to the non-fused construct. After applying this fusion strategy in insect-resistant rice breeding, recombinant OsrbcS-Cry1Ab/Cry1Ac expressed rice plants conferred high resistance to leaffolders and striped stem borers, among which two single-copy lines possessed normal agronomic performance in the field. Specifically, Cry1Ab/Cry1Ac protein levels in single-copy construct transgenic lines ranged from 1.8 to 11.5 µg g−1 in the leaf, higher than the Actin I promoter-driven control, T51-1, about 1.78 µg g−1 in the leaf, but negligible (only 0.00012–0.00117 µg g−1) in endosperm by ELISA analysis. Our study provided a novel approach to creating Cry1Ab/Cry1Ac-free endosperm rice with a high level of insect-resistant protein in green tissues through the simultaneous usage of the OsrbcS promoter and OsrbcS as a fusion partner.
Investigating Mammalian Formins with SMIFH2 Fifteen Years in: Novel Targets and Unexpected Biology
Authors: Innocenti Metello
Published: May 2023

The mammalian formin family comprises fifteen multi-domain proteins that regulate actin dynamics and microtubules in vitro and in cells. Evolutionarily conserved formin homology (FH) 1 and 2 domains allow formins to locally modulate the cell cytoskeleton. Formins are involved in several developmental and homeostatic processes, as well as human diseases. However, functional redundancy has long hampered studies of individual formins with genetic loss-of-function approaches and prevents the rapid inhibition of formin activities in cells. The discovery of small molecule inhibitor of formin homology 2 domains (SMIFH2) in 2009 was a disruptive change that provided a powerful chemical tool to explore formins’ functions across biological scales. Here, I critically discuss the characterization of SMIFH2 as a pan-formin inhibitor, as well as growing evidence of unexpected off-target effects. By collating the literature and information hidden in public repositories, outstanding controversies and fundamental open questions about the substrates and mechanism of action of SMIFH2 emerge. Whenever possible, I propose explanations for these discrepancies and roadmaps to address the paramount open questions. Furthermore, I suggest that SMIFH2 be reclassified as a multi-target inhibitor for its appealing activities on proteins involved in pathological formin-dependent processes. Notwithstanding all drawbacks and limitations, SMIFH2 will continue to prove useful in studying formins in health and disease in the years to come.