bluuebunny/crossref_metadata_2025 huggingface dataset:
Antibodies against endogenous retroviruses
Authors: Chisca Mihaela , Larouche Jean‐David , Xing Qi , Kassiotis George
Published: August 2024

The human genome harbors hundreds of thousands of integrations of ancient retroviruses, amassed over millions of years of evolution. To reduce further amplification in the genome, the host prevents transcription of these now endogenous retroviruses (ERVs) through epigenetic repression and, with evolutionary time, ERVs are incapacitated by accumulating mutations and deletions. However, several members of recently endogenized ERV groups still retain the capacity to produce viral RNA, retroviral proteins, and higher order structures, including virions. The retention of viral characteristics, combined with the reversible nature of epigenetic repression, particularly as seen in cancer, allow for immunologically unanticipated ERV expression, perceived by the adaptive immune system as a genuine retroviral infection, to which it has to respond. Accordingly, antibodies reactive with ERV antigens have been detected in diverse disorders and, occasionally, in healthy individuals. Although they are part of self, the retroviral legacy of ERV antigens, and association with and, possibly, causation of disease states may set them apart from typical self‐antigens. Consequently, the pathogenic or, indeed, host‐protective capacity of antibodies targeting ERV antigens is likely to be context‐dependent. Here, we review the immunogenicity of typical ERV proteins, with emphasis on the antibody response and its potential disease implications.
Complexant‐facilitated assembly of NiTiO3 nanoparticles into microbars for high‐performance lithium‐ion battery anode
Authors: Sun Meng , Sheng Xiaoli , Cui Zhipeng , Li Sijie , Zhang Qingye , Xie Fei , Liu Guanting , Hao Shujin , Diao Feiyu , Sun Shiduo , Wang Yiqian
Published: August 2024

Nickel titanate (NiTiO3) nanostructured materials have gained extensive attention in the field of lithium‐ion batteries (LIBs) due to their high theoretical capacity and low cost. However, NiTiO3 exhibits low conductivity and significant volume changes during cycling, resulting in capacity decay and poor cycling stability. Herein, we propose a feasible strategy to enhance the cycling performance of NiTiO3 nanostructures by adjusting their morphology. By manipulating the choice of solvent employed in the synthetic process, we obtain NiTiO3 microbars (NTO MBs) through self‐assembly of NiTiO3 nanoparticles (NTO NPs). When utilized as an anode material in LIBs, NTO MBs exhibit a capacity of 410 mAh g−1 after 200 cycles at 100 mA g−1, surpassing that of NTO NPs (212 mAh g−1). The improved performance of NTO MBs is attributed to their unique porous bar‐like structure, composed of numerous NPs, which provides a substantial storage space for Li+ ions owing to its larger specific surface area. Additionally, the porous structure accelerates the diffusion of Li+ ions and electron transfer. To gain a profound understanding of the enhanced performance through morphology adjustment, we conduct a comprehensive investigation on the growth mechanism of NTO MBs. This work provides valuable insights into the mechanism governing the morphology control of NTO MBs, facilitating the rational design and synthesis of tailored materials with enhanced performance for LIBs.
The Role of Interleukin‐8 in the Estimation of Responsiveness to Steps 1 and 2 of Periodontal Therapy
Authors: Bumm Caspar Victor , Schwendicke Falk , Heck Katrin , Frasheri Iris , Summer Burkhard , Ern Christina , Heym Richard , Werner Nils , Folwaczny Matthias
Published: August 2024

Objective

To investigate the association between interleukin‐8 (IL‐8) levels in gingival crevicular fluid (GCF) and total oral fluid (TOF) and the responsiveness to steps 1 and 2 of periodontal therapy.

Materials and Methods

One‐hundred and fifty‐nine patients affected by periodontitis received steps 1 and 2 of periodontal therapy. At baseline, TOF and GCF samples were collected and analysed for IL‐8 (Il‐8TOF/IL‐8GCF) using flow cytometry. Therapy outcomes were relative proportions of residual periodontal pockets (PPD%), pocket closure (PC) rates and pocket probing depth (PPD) reductions; these were associated with IL‐8TOF/IL‐8GCF.

Results

High IL‐8TOF was significantly associated with higher residual PPD% (p = 0.044) and lower PPD reduction compared to low IL‐8TOF (high 0.79 ± 1.20 mm vs. low 1.20 ± 1.20 mm, p < 0.001) in non‐smokers, while in smokers high IL‐8GCF was related to lower PPD reduction (high 0.62 ± 1.22 mm vs. low 0.84 ± 1.12 mm, p = 0.009). Furthermore, high baseline IL‐8TOF was significantly associated with poorer PC rates compared to medium and low concentrations in both non‐smokers (high 41% vs. medium 55% vs. low 58%, p < 0.001) and smokers (high 34% vs. medium 44% vs. low 46%, p < 0.001).

Conclusion

High IL‐8 concentrations at baseline had a significant impact on residual PPD%, PC rates and PPD reduction. The findings suggest that, especially in non‐smokers, baseline IL‐8 levels collected from the TOF could serve as a component in the estimation of responsiveness to steps 1 and 2 of periodontal therapy.
Association of Oral Microbiome Diversity and All‐Cause Mortality in the General US Population and in Individuals With Chronic Diseases: A Prospective Cohort Study
Authors: Yang Zhiwen , He Fengling , Huang Haoxiang , Xu Junyang , Ruan Yifei , Cui Kai , Zhou HuiLei , Chen Yijin , Liu Dan , Xiao Zhiwen , Chen Feng , Liao Yulin , Bin Jianping , Chen Yanmei
Published: August 2024

Aim

To investigate whether oral microbiome diversity is associated with all‐cause mortality in the general US population and in individuals with chronic diseases.

Materials and Methods

We included 8224 individuals with oral microbiome diversity data from the National Health and Nutrition Examination Survey (2009–2012), representing 164,000,205 US adults, using a survey‐weighted analysis method. Cox regression analyses were performed to identify the association between oral microbiome diversity and all‐cause mortality.

Results

During a survey‐weighted mean follow‐up period of 8.86 years, 429 all‐cause deaths (survey‐weighted number: 7,124,920) occurred in 8224 participants. Cox regression analysis revealed that higher oral microbiome diversity was significantly associated with a lower all‐cause mortality risk. Significant differences in all‐cause mortality risk were observed among the different clusters based on oral microbiome β‐diversity (log‐rank p < 0.001). Subgroup analyses revealed that the oral microbiome diversity was independently associated with all‐cause mortality in individuals with diabetes mellitus and hypertension. A multivariate logistic regression model showed that current smoking and antibiotic use were significantly associated with lower oral microbiome α diversity.

Conclusions

Higher oral microbiome diversity was significantly associated with a lower all‐cause mortality risk in the general US population and in individuals with diabetes mellitus and hypertension.
Scientific validation of plant extracts used by farmers in the management of bean flower thrips on French beans in Kenya
Authors: Munyoki Nancy Mwende , Muthomi James , Kilalo Dora , Bautze David , Kiboi Milka , Mwangi Edwin , Karanja Edward , Matheri Felix , Adamtey Noah , Akutse Komivi S. , Cheseto Xavier
Published: August 2024

Bean flower thrips (Megalurothrips usitatus) is a major French bean (Phaseolus vulgaris L.) pest. Small‐scale farmers manage the pest using mixed plant extracts although their efficacy has not been scientifically validated. We evaluated the efficacy of mixed plant extracts comprising; Capsicum frutescens, Allium sativum, Lantana camara, Tagetes minuta and Azadirachta indica, against M. usitatus under laboratory and screenhouse. We identified and quantified the secondary metabolites associated with insecticidal activity using spectrophotometry and liquid chromatography–mass spectrometry (LC–MS). The plant combinations included PE1 (C. frutescens + A. sativum + L. camara + T. minuta extracts infused for 14 days), PE2 (same as PE1 but infused for 24 h) and PE + N (the five plant extracts infused for 24 h) in distilled water. We used an organic commercial botanical (Pyneem) as a positive control and distilled water as a negative control. Pyneem and PE + N induced the highest mortality at 88% and 77%, respectively, in the laboratory, and 68% and 71%, respectively, in the screenhouse. Phenolics, terpenoids and organosulfur compounds were identified in PE + N and individual plant extracts in varied quantities. These compounds were significantly higher (p < 0.001) in PE + N compared to individual plant extracts. The study showed that PE + N efficiently manages bean flower thrips, and mixing different plant extracts amplifies the secondary metabolites' abundance. The use of mixed plant extracts could be incorporated into integrated pest management strategies for thrips management in legumes. The specific compounds identified in PE + N should be investigated further to understand their modes of action against the pest.
Drivers of growth in strong year classes of the deepwater redfish (Sebastes mentella) population from the Gulf of St. Lawrence derived from otolith increment‐based growth chronologies
Authors: Coussau Lola , Morissette Olivier , Robert Dominique , Sirois Pascal
Published: August 2024

The case of the deepwater redfish (Sebastes mentella) in the Gulf of St. Lawrence (GSL) is a compelling example of drastic fluctuations in annual recruitment strength, characteristic of spasmodic stocks. After three decades of low abundance, the emergence of three consecutive strong year classes in 2011–2013 resulted in an unprecedented increase in biomass. In spasmodic stocks such as GSL redfish, strong year classes sustain both the biomass and catch for decades. Therefore, understanding the growth dynamics of these cohorts is essential. In the present study, we reconstructed the annual growth rates of redfish using otolith increment‐based annual chronology and investigated the drivers of growth variation in redfish strong year classes of the early 2010s and early 1980s. Stock biomass was identified as the main extrinsic driver of redfish growth, suggesting intense competition for food at high conspecific density. Warming of deep waters in the GSL, where adult redfish settle, positively correlated with individual growth. However, recent warming of the cold intermediate layer showed a negative correlation with redfish growth, likely related to the shrinking of the habitat this water mass provides for various redfish cold‐water prey rather than to a direct effect of temperature. Reconstruction of redfish annual growth trajectories from birth to capture emphasized the importance of carryover effects in the growth potential of strong year classes. This work provided an important first outlook of the factors driving growth variation in GSL redfish spasmodic stock and explored midterm consequences of density‐dependent pressures on biological parameters of the population.
Endoscopic submucosal dissection for lesions developing in the irradiated area of head and neck cancer
Authors: Tani Yasuhiro , Kanesaka Takashi , Urabe Kosuke , Korematsu Mizuki , Kitamura Koji , Miyabe Junji , Otozai Shinji , Yoshii Tadashi , Kato Minoru , Yoshii Shunsuke , Michida Tomoki , Ishihara Ryu , Konishi Koji , Honma Keiichiro , Fujii Takashi
Published: August 2024

Background and Aim

Effective treatment of lesions that develop in the irradiated area of head and neck squamous cell carcinoma is a major concern. This study aimed to clarify the efficacy and safety of endoscopic resection for such lesions.

Methods

Among consecutive patients who underwent endoscopic resection for histologically proven head and neck squamous cell carcinoma between January 2014 and December 2021, those who received definitive radiotherapy/chemoradiotherapy before endoscopic resection were included in this single‐center, retrospective study. Short‐ and long‐term outcomes were evaluated.

Results

Among 422 patients who underwent endoscopic resection for 615 lesions, 43 patients with 57 lesions were eligible. All 57 lesions were treated with endoscopic submucosal dissection and en bloc resection was achieved in all lesions. Grade 3 of Common Toxicity Criteria for Adverse Events v5.0 occurred in eight (19%) patients (dysphagia, seven; stricture, three; aspiration pneumonia, two; and pharyngeal necrosis, one [some cases overlapped]), but no grade ≥ 4 events occurred. Enteral nutrition by gastrostomy was temporarily required in two patients owing to dysphagia and laryngeal necrosis. During the median follow‐up of 40 (interquartile range, 29.5–61) months after endoscopic submucosal dissection for the lesions developed in the irradiated area, local recurrence and metachronous lesions developed in two (5%) and nine (21%) patients, respectively. However, total laryngectomies and tracheostomies were avoided in all patients. The 3‐year overall and disease‐specific survivals were 81% (95% confidence interval, 64%–91%) and 94% (95% confidence interval, 79%–99%), respectively.

Conclusions

Favorable local control and safety of endoscopic submucosal dissection were demonstrated.
Quantitation of the physicochemical properties of myelin using Nile Red fluorescence spectroscopy
Authors: Teo W. , Morgan M. L. , Stys P. K.
Published: August 2024

Myelin is a vital structure that is key to rapid saltatory conduction in the central and peripheral nervous systems. Much work has been done over the decades examining the biochemical composition and morphology of myelin at the light and electron microscopic levels. Here we report a method to study myelin based on the fluorescent probe Nile Red. This lipophilic dye readily partitions into live and chemicallyfixed myelin producing bright, well‐resolved images of the sheath. Using spectral confocal microscopy, a complete emission spectrum of Nile Red fluorescence can be acquired for each pixel in an image. The solvatochromic properties of Nile Red cause its emission spectrum to change depending on the polarity of its local environment. Therefore, measuring spectral shifts can report subtle changes in the physicochemical properties of myelin. We show differences in myelin polarity in central versus peripheral nervous system and in different regions of central nervous system white matter of the mouse brain, together with developmental and sex variations. This technique is also well suited for measuring subtle changes in myelin properties in live ex vivo white matter specimens. We also demonstrate how light deprivation induces a myelin polarity change in adult mouse optic nerve underscoring a continuing myelin plasticity in response to axonal activity well into adulthood. The Nile Red spectroscopic method allows measurement of subtle physicochemical changes in myelin that can importantly influence its electrical properties and by extension, conduction velocities in axons.

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Long‐term and low‐dose rituximab treatment for chronic inflammatory demyelinating polyneuropathy
Authors: Zheng Yongsheng , Sun Chong , Zhao Yanyin , Meng Quanhua , Hu Jianian , Qiao Kai , Sun Jian , Xi Jianying , Luo Sushan , Lu Jiahong , Zhao Chongbo , Lin Jie
Published: August 2024

Objective

To evaluate the efficacy and safety of a low‐dose, long‐term rituximab regimen in the treatment of idiopathic CIDP.

Methods

This study included 15 CIDP patients treated with rituximab. Patients were administered 600 mg of rituximab intravenously every 6 months. Baseline evaluation was conducted before the initiation of rituximab treatment and subsequent evaluations were conducted 6 months after each rituximab infusion at on‐site visits. Clinical improvement was objectively determined by improvement of scale score at least decrease ≥1 INCAT or mRS or increase ≥4 MRC or ≥8 cI‐RODS after each infusion compared to baseline evaluation.

Results

Fifteen CIDP patients were included and 10 of them were typical CIDP and five were distal CIDP. Nine in 15 (60%) patients after first infusion and three in six (50%) patients after second infusion exhibited significant clinical improvement compared to baseline evaluation. Additionally, rituximab facilitated a reduction or cessation of other medications in 73% of patients at last visit. The safety profile was favorable, with no reported adverse events.

Conclusion

Rituximab presents a promising therapeutic option for idiopathic CIDP, offering both efficacy and safety with a low‐dose, long‐term regimen.
Anatomical description of neornithine stomach with implications on neornithine stomach morphology
Authors: Takasaki Ryuji , Kobayashi Yoshitsugu
Published: August 2024

Neornithines, the most diversified extant tetrapods, have been a classic example for understanding form–function relationships, particularly in the context of the interaction between dietary ecology and neornithine phenotypic evolution. While the previous studies have primarily focused on beak morphology, the significance of the neornithine stomach, which serves as a functional analog of mammalian dentition, is expected to play an important role as well. However, current knowledge on neornithine stomachs is predominantly biased toward poultry and birds of prey, leading to a significant underappreciation of its impact on macroevolution. Here, we provide detailed descriptions of neornithine stomachs represented by 115 species of major orders and test if and how neornithine stomachs are related to their dietary ecology. We identified four morphotypes among neornithine stomachs, which are strongly constrained phylogenetically. While the neornithine diet demonstrates strong associations with stomach morphotypes, the associations are small or absent when accounting for the phylogeny in statistical evaluations. Similarly, the neornithine diet has negligible effects on their ventriculus mass under the analyses with phylogenetic correction. The results resemble a recent finding that a neornithine diet has no effect on intestine length when accounting for phylogeny, but rather flight performance does. Thus, the present study further supports the previous findings that dietary specialization in neornithine birds closely follows phylogeny, making functional convergence across taxa difficult to detect.
Persisting gaps in dementia carer wellbeing and education: A qualitative exploration of dementia carer experiences
Authors: White Jennifer , Falcioni Dane , Barker Roslyn , Bajic‐Smith Julie , Krishnan Chitra , Mansfield Elise , Hullick Carolyn
Published: August 2024

Aims

To explore the emotional wellbeing of dementia carers in the lead up to and during transition of a person living with dementia to a residential aged care facility.

Design

An interpretative qualitative study.

Methods

Semi‐structured interviews were conducted with informal carers of person living with dementia between February and June 2023. Data were analysed using an inductive thematic approach and resulted in three themes.

Results

The majority of carers were adult children (n = 19) and six were wives. Carers lived across metropolitan (n = 20) and regional settings (n = 5) in the most populous state of Australia. Three themes were identified which were attributed to different aspects of the carer role: (1) Carer emotional journey as dementia progresses – impacted by knowledge and lack of support; (2) Questioning decision making–underpinned by knowledge and confidence; and (3) Challenges in re‐establishing identity – impacted by ongoing concerns.

Conclusion

As dementia progresses carers of person living with dementia consistently reported gaps in knowledge including how to access support. Specifically, this study identified the need for more to be done to help carers to develop the skills needed for their role, including participation in care planning and identifying care preferences for the future. Nurses can play a key role in promoting referral to services that support carers. Findings offer practical solutions to ameliorate carer stress and promote shared decision making.

Reporting Method

This research was guided by the Consolidated Criteria for Reporting Qualitative Research.
Practice‐based research in dentistry: Exploring methodologies, benefits, and challenges
Authors: Mungia Rahma , Funkhouser Ellen , Lobbezoo Frank , Verhoeff Merel Charlotte , Gilbert Gregg H. ,   National Dental PBRN Collaborative Group
Published: August 2024

Background

Practice‐Based Research Network (PBRN) studies typically assess the effectiveness of clinical interventions in settings that match real‐world conditions. Dental PBRNs have the capacity to amass, identify, and analyze vast amounts of data from patient populations that include diverse racial, ethnic, socio‐economic, and geographic backgrounds. These dental studies encompass a broad spectrum of healthcare aspects, including prevention, diagnosis, symptom and disease treatment, quality enhancement, and care coordination.

Methods

An extensive range of research methodologies can be employed within dental PBRNs to investigate these topics, including randomized controlled trials. Dental PBRNs have evolved from primarily focusing on case observations to leveraging advanced network infrastructure and collaborating across multiple regional and national sites. In addition to producing numerous high‐impact peer‐reviewed publications, study results have led to improved clinical care. However, PBRNs encounter challenges, such as the sustainability of research capacity (relying heavily on ongoing support from funding agencies), diverse research cultures, and an imperative to design studies that are both feasible and relevant to everyday clinical practice. Recognizing the pivotal role of real‐world evidence, it is important to have sustained investment in dental PBRN infrastructure and feasible opportunities for practitioners to participate in network activities nationwide.

Conclusion

Practice‐Based Research Network studies capitalize on an important research context within which to investigate a range of clinical topics that can employ multiple research methodologies. However, sustaining productive networks requires strategic effort, ongoing financial support, and customized organizational skills.
Intramuscular condition of swallowing‐related muscles using shear‐wave elastography: A preliminary study in healthy adults
Authors: Ichikawa Yoko , Kikutani Takeshi , Sumi Misa , Iwata Hiroshi , Takahashi Noriaki , Tohara Takashi , Ozeki Maiko , Tamura Fumiyo
Published: August 2024

Background

Assessing the internal condition of swallowing‐related muscles and muscle strength and size is important because their deterioration may lead to dysphagia. However, there are few reports on stiffness of swallowing‐related muscles measured using shear‐wave elastography (SWE) and their qualitative characteristics.

Objectives

We measured stiffness of swallowing‐related muscles using SWE to investigate the relationship between muscle stiffness and body composition as well as other relevant variables in healthy adults.

Methods

Thirty healthy adults were included in this cross‐sectional study. We evaluated stiffness of the genioglossus muscle (GGM) and geniohyoid muscle (GHM) using SWE. Skeletal muscle mass index, body mass index (BMI), handgrip strength, tongue pressure, and cross‐sectional areas of the muscles were measured, and we determined their relationship with muscle stiffness.

Results

For muscle stiffness, the mean value for the middle GGM was 7.08 ± 1.92 kPa, that for the posterior GGM was 9.31 ± 2.68 kPa, and that for the GHM was 8.84 ± 2.15 kPa. In multiple regression analysis, with stiffness of the posterior GGM as the dependent variable, BMI (β = −.473, p = .039) was a significant negative explanatory variable. However, with the GHM stiffness as the dependent variable, BMI (β = .419, p = .033) was a significant positive explanatory variable.

Conclusion

Stiffness of the posterior GGM was positively correlated with BMI and that of the GHM was negatively correlated with BMI. Stiffness, as measured using SWE, has the potential to capture the intramuscular characteristics of swallowing‐related muscles, particularly the posterior GGM.
The Effects of Multifaceted Introversion and Sensory Processing Sensitivity on Solitude‐Seeking Behavior
Authors: Thomas Virginia , Nelson Paul A.
Published: August 2024

Introduction

The state of solitude may be desirable and beneficial particularly for individuals who are highly sensitive and introverted.

Methods

To test these predictions, we surveyed a nationally representative US sample of 301 adults and a sample of 99 undergraduates on their levels of sensory processing sensitivity and assessed introversion with the Big Five Inventory and the multifaceted STAR Introversion Scale. Participants then reported the frequency and duration of their volitional solitude, stress levels, and subjective well‐being across 10 consecutive days.

Results

Results revealed that Social Introversion and sensitivity significantly predicted higher motivations for solitude, both self‐determined and not. Thinking Introversion also predicted higher self‐determined solitude, but BFI introversion showed no relationship with either motivation. Social Introversion and sensitivity predicted higher frequency of solitude in daily life and longer duration of solitary episodes; BFI Introversion and Restrained Introversion showed the opposite pattern for both outcomes. Finally, stress was positively associated with daily solitude frequency, and in turn, solitude frequency was negatively associated with same‐day well‐being; there were no interaction effects with personality traits.

Discussion

These findings suggest that introversion, as measured by the STAR Introversion Scale, and sensitivity contribute significantly to solitary motivation; however, solitude appears to be sought after by people in times of stress regardless of their scores on these traits.
We are doing it wrong: Putting homology before phylogeny in cyanobacterial taxonomy
Authors: Villanueva Chelsea D. , Bohunická Markéta , Johansen Jeffrey R.
Published: August 2024

The rapid expansion of whole genome sequencing in bacterial taxonomy has revealed deep evolutionary relationships and speciation signals, but assembly methods often miss true nucleotide diversity in the ribosomal operons. Though it lacks sufficient phylogenetic signal at the species level, the 16S ribosomal RNA gene is still much used in bacterial taxonomy. In cyanobacterial taxonomy, comparisons of 16S–23S Internal Transcribed Spacer (ITS) regions are used to bridge this information gap. Although ITS rRNA region analyses are routinely being used to identify species, researchers often do not identify orthologous operons, which leads to improper comparisons. No method for delineating orthologous operon copies from paralogous ones has been established. A new method for recognizing orthologous ribosomal operons by quantifying the conserved paired nucleotides in a helical domain of the ITS, has been developed. The D1′ Index quantifies differences in the ratio of pyrimidines to purines in paired nucleotide sequences of this helix. Comparing 111 operon sequences from 89 strains ofBrasilonema, four orthologous operon types were identified. Plotting D1′ Index values against the length of helices produced clear separation of orthologs. Most orthologous operons in this study were observed both with and without tRNA genes present. We hypothesize that genomic rearrangement, not gene duplication, is responsible for the variation among orthologs. This new method will allow cyanobacterial taxonomists to utilize ITS rRNA region data more correctly, preventing erroneous taxonomic hypotheses. Moreover, this work could assist genomicists in identifying and preserving evident sequence variability in ribosomal operons, which is an important proxy for evolution in prokaryotes.
Prevalence and distribution of carbapenem‐resistant Enterobacterales in companion animals: A nationwide study in the United States using commercial laboratory data
Authors: Sobkowich Kurtis , Poljak Zvonimir , Weese J. Scott , Plum Andy , Szlosek Donald , Bernardo Theresa M.
Published: August 2024

Background

Carbapenem‐resistant Enterobacterales (CRE) are a concern in both human and animal medicine globally. Despite extensive research in humans, limited data exist on CRE in companion animals, with a lack of nationwide prevalence estimates.

Hypothesis/Objectives

To assess the occurrence and trends of CRE in cats and dogs across the United States by analyzing 4 years of commercial antimicrobial susceptibility testing (AST) data.

Animals

Between 2019 and 2022, 477 426 ASTs were conducted on Enterobacterales isolates against imipenem. Isolates were derived from 379 598 dogs and 97 828 cats. Animal origin was not disclosed.

Methods

In this retrospective study, antimicrobial susceptibility test data from IDEXX Laboratories were analyzed. Analysis included resistance estimations to imipenem stratified by sampling site, an assessment of resistance patterns over time and location, and the application of space‐time cluster analysis to identify potential outbreaks. Antibiograms were produced for carbapenem‐resistant isolates.

Results

Susceptibility to imipenem was high, at 98.86%. Temporal analysis indicated stability in susceptibility, with an unexplained reduction in susceptible isolates in June 2019. Spatial analysis identified 2 high‐risk clusters along the Western Coast (relative risk [RR]: 23.26; P < .001) and in Texas (RR: 10.72; P < .001) in that month. Three other clusters were found, in Missouri (RR: 39.55; P = .038), Florida (RR: 4.53; P < .001), and New York (RR: 9.20; P < .001).

Conclusions and Clinical Importance

CRE are present at a low prevalence in dogs and cats across the United States. Variations in prevalence across patient‐level and environmental factors highlight the need for tailored stewardship programs.
Meiofaunal Dynamics in Oceanic Islands: Insights From Spatial Distribution, Substrate Influence and Connectivity
Authors: Torres‐Martínez Adrián , Riera Rodrigo
Published: August 2024

There is an apparent contradiction between the sedentary lifestyle and direct development of meiofaunal organisms and their widely observed distribution. This study is situated within the theoretical framework of ecological connectivity, metacommunities, and the impact of substrate type, particularly in the unique context of oceanic islands, offering a crucial perspective to unravel the complexities of meiofaunal dispersal and connectivity. We here studied the spatial distribution of meiofauna on natural and artificial beaches of an oceanic island, that is, Gran Canaria (Canary Is., NE Atlantic Ocean). The results revealed higher abundance and richness of meiofauna on artificial substrates compared to natural ones, with nematodes, copepods, and annelids being the dominant groups. The meiofaunal community composition differed significantly between natural and artificial beaches, suggesting colonisation of artificial beaches by natural beach communities. Notably, certain species were exclusively found on artificial beaches. The study highlighted the importance of substrate type in influencing meiofaunal composition, with artificial beaches providing new ecological niches and resources for meiofauna. The dissimilarity between beaches was attributed to morphospecies exclusive to either artificial or natural substrates, emphasising the role of dispersal mechanisms. These findings contribute to understanding the metacommunity dynamics of meiofauna in oceanic islands and call for further research on dispersal potential and biogeography.
Glass ceiling, sticky floor, or both? Public and private sector differences in Türkiye
Authors: Rios‐Avila Fernando , Özekin Ayça , Komuryakan Fulden
Published: August 2024

This study examines the glass ceiling and sticky floor phenomena within the contexts of both the public and private sectors in Türkiye, while exploring sector‐specific differences. To do this, we use a recentered influence function‐based decomposition method to analyze data from the Household Labor Force Statistics spanning from 2014 to 2021. Our findings suggest that the sticky floor effect emerges as more prominent in the private sector, contrasting with the public sector where the glass ceiling effect holds greater sway. Additionally, our analysis reveals that gender discrimination appears to be more prevalent in the private sector, although it exhibits an increasing trend in the public sector. The evidence suggests a need for further research on the gender wage gap in Türkiye, especially within the context of the glass ceiling and sticky floor effects. This study contributes to the existing literature by offering a comprehensive examination of these phenomena in both public and private sectors, leveraging the availability of recent data and advancements in methodologies.
Automated acoustic event‐based monitoring of prestressing tendons breakage in concrete bridges
Authors: Farhadi Sasan , Corrado Mauro , Ventura Giulio
Published: August 2024

Prestressing wire breakage induced by corrosion is hazardous, especially for concrete structures subjected to severe aging factors, such as bridges. Developing an automated monitoring system for such a damage event is therefore essential for ensuring structural integrity and preventing catastrophic failures. In line with this target, a supervised deep learning–based approach is proposed to detect and classify acoustic emissions released by prestressing wire breakage. The application of advanced signal processing techniques is central to this study to determine optimal model performance and accurately detect patterns of various events. Diverse pretrained convolutional neural network (CNN) architectures are explored and further enhanced by incorporating Bottleneck Attention Mechanisms to refine their performance capabilities. Additionally, a novel hybrid model, AcousticNet, tailored for acoustic event classification in the context of structural health monitoring, is developed. The models are trained and validated using an extensive data set collected from controlled laboratory experiments and in situ bridge monitoring scenarios, ensuring comprehensive adaptability and generalizability. The comprehensive analysis highlights that the Xception model, enhanced with a bottleneck module, and AcousticNet significantly outperform other models in capturing intricate patterns within acoustic signals. Integrating advanced CNN architectures with signal processing methods marks a substantial advancement in the automated monitoring of prestressed concrete bridges.
Prediction of stratified ground consolidation via a physics‐informed neural network utilizing short‐term excess pore water pressure monitoring data
Authors: Gong Weibing , Zuo Linlong , Li Lin , Wang Hui
Published: August 2024

Predicting stratified ground consolidation effectively remains a challenge in geotechnical engineering, especially when it comes to quickly and dependably determining the coefficient of consolidation () for each soil layer. This difficulty primarily stems from the time‐intensive nature of the consolidation process and the challenges in efficiently simulating this process in laboratory settings and using numerical methods. Nevertheless, the consolidation of stratified ground is crucial because it governs ground settlement, affecting the safety and serviceability of structures situated on or in such ground. In this study, an innovative method utilizing a physics‐informed neural network (PINN) is introduced to predict stratified ground consolidation, relying solely on short‐term excess pore water pressure (PWP) data collected by monitoring sensors. The proposed PINN framework identifies from the limited PWP data set and subsequently utilizes the identified to predict the long‐term consolidation process of stratified ground. The efficacy of the method is demonstrated through its application to a case study involving two‐layer ground consolidation, with comparisons made to an existing PINN method and a laboratory consolidation test. The results of the case study demonstrate the applicability of the proposed PINN method to both forward and inverse consolidation problems. Specifically, the method accurately predicts the long‐term dissipation of excess PWP when is known (i.e., the forward problem). It successfully identifies the unknown with only 0.05‐year monitoring data comprising 10 data points and predicts the dissipation of excess PWP at 1‐year, 10‐year, 15‐year, and even up to 30‐year intervals using the identified (i.e., the inverse problem). Moreover, the investigation into optimal PWP monitoring sensor layouts reveals that installing sensors in areas with significant variations in excess PWP enhances the prediction accuracy of the proposed PINN method. The results underscore the potential of leveraging PINNs in conjunction with PWP monitoring sensors to effectively predict stratified ground consolidation.
The potential for an increasing threat of unseasonal temperature cycles to dormant plants
Authors: Kovaleski Al P.
Published: August 2024

Two functional responses largely guide woody plants' survival to winter conditions: cold hardiness and dormancy. Dormancy affects budbreak timing based on chill accumulation. Effects of warming on dormancy may appear time‐shifted: fall and winter warming events decrease chill accumulation, delaying budbreak observed in spring. The same warming events also affect cold hardiness dynamics, having immediate implications. As cold deacclimation rates increase with dormancy progression, the same amount of warming has greater damage risk the later it occurs in the season, depending on return of low temperatures. Should frequency of erratic weather increase with climate change, more instances of risk are expected. However, understanding how plants fare through seasons now and in future climates still requires better knowledge of winter physiology.
Interoceptive training with real‐time haptic versus visual heartbeat feedback
Authors: Dobrushina Olga , Tamim Yossi , Wald Iddo Yehoshua , Maimon Amber , Amedi Amir
Published: August 2024

The perception of signals from within the body, known as interoception, is increasingly recognized as a prerequisite for physical and mental health. This study is dedicated to the development of effective technological approaches for enhancing interoceptive abilities. We provide evidence of the effectiveness and practical feasibility of a novel real‐time haptic heartbeat supplementation technology combining principles of biofeedback and sensory augmentation. In a randomized controlled study, we applied the developed naturalistic haptic feedback on a group of 30 adults, while another group of 30 adults received more traditional real‐time visual heartbeat feedback. A single session of haptic, but not visual heartbeat feedback resulted in increased interoceptive accuracy and confidence, as measured by the heart rate discrimination task, and in a shift of attention toward the body. Participants rated the developed technology as more helpful and pleasant than the visual feedback, thus indicating high user satisfaction. The study highlights the importance of matching sensory characteristics of the feedback provided to the natural bodily prototype. Our work suggests that real‐time haptic feedback might be a superior approach for strengthening the mind–body connection in interventions for physical and mental health.
Seed color patterns in domesticated common bean are regulated by MYB‐bHLH‐WD40 transcription factors and temperature
Authors: Parker Travis , Bolt Tayah , Williams Troy , Penmetsa R. Varma , Mulube Mwiinga , Celebioglu Burcu , Palkovic Antonia , Jochua Celestina Nhagupana , del Mar Rubio Wilhelmi Maria , Lo Sassoum , Bornhorst Gail , Tian Li , Kamfwa Kelvin , Farmer Andrew , Diepenbrock Christine , Gepts Paul
Published: August 2024

Seed colors and color patterns are critical for the survival of wild plants and the consumer appeal of crops. In common bean, a major global staple, these patterns are also essential in determining market classes, yet the genetic and environmental control of many pigmentation patterns remains unresolved. In this study, we genetically mapped variation for several important seed pattern loci, including T, Bip, phbw, and Z, which co‐segregated with candidate genes PvTTG1, PvMYC1, PvTT8, and PvTT2, respectively. Proteins encoded by these genes are predicted to work together in MYB‐bHLH‐WD40 (MBW) complexes, propagating flavonoid biosynthesis across the seed coat as observed in Arabidopsis. Whole‐genome sequencing of 37 accessions identified mutations, including seven unique parallel mutations in T (PvTTG1) and non‐synonymous SNPs in highly conserved residues in bipana (PvMYC1) and z (PvTT2). A 612 bp intron deletion in phbw (PvTT8) eliminated motifs conserved since the Papilionoideae origin and corresponded to a 20‐fold reduction in transcript abundance. In multi‐location field trials of seven varieties with partial seed coat pigmentation patterning, the pigmented seed coat area correlated positively with ambient temperature, with up to 11‐fold increases in the pigmented area from the coolest to the warmest environments. In controlled growth chamber conditions, an increase of 4°C was sufficient to cause pigmentation on an average additional 21% of the seed coat area. Our results shed light on key steps of flavonoid biosynthesis in common bean. They will inform breeding efforts for seed coat color/patterning to improve consumer appeal in this nutritious staple crop.
Extension of planar Hölder homeomorphisms
Authors: Hencl Stanislav , Koski Aleksis
Published: August 2024

Let . We show that any ‐Hölder homeomorphism from the unit circle in the plane to the plane can be extended to an ‐Hölder homeomorphism from the whole unit disc.
Interference with glutamate antiporter system xc− enables post‐hypoxic long‐term potentiation in hippocampus
Authors: Heit Bradley S. , Chu Alex , McRay Alyssa , Richmond Janet E. , Heckman Charles J. , Larson John
Published: August 2024

Our group previously showed that genetic or pharmacological inhibition of the cystine/glutamate antiporter, system xc−, mitigates excitotoxicity after anoxia by increasing latency to anoxic depolarization, thus attenuating the ischaemic core. Hypoxia, however, which prevails in the ischaemic penumbra, is a condition where neurotransmission is altered, but excitotoxicity is not triggered. The present study employed mild hypoxia to further probe ischaemia‐induced changes in neuronal responsiveness from wild‐type and xCT KO (xCT−/−) mice. Synaptic transmission was monitored in hippocampal slices from both genotypes before, during and after a hypoxic episode. Although wild‐type and xCT−/− slices showed equal suppression of synaptic transmission during hypoxia, mutant slices exhibited a persistent potentiation upon re‐oxygenation, an effect we termed ‘post‐hypoxic long‐term potentiation (LTP)’. Blocking synaptic suppression during hypoxia by antagonizing adenosine A1 receptors did not preclude post‐hypoxic LTP. Further examination of the induction and expression mechanisms of this plasticity revealed that post‐hypoxic LTP was driven by NMDA receptor activation, as well as increased calcium influx, with no change in paired‐pulse facilitation. Hence, the observed phenomenon engaged similar mechanisms as classical LTP. This was a remarkable finding as theta‐burst stimulation‐induced LTP was equivalent between genotypes. Importantly, post‐hypoxic LTP was generated in wild‐type slices pretreated with system xc− inhibitor, S‐4‐carboxyphenylglycine, thereby confirming the antiporter's role in this phenomenon. Collectively, these data indicate that system xc− interference enables neuroplasticity in response to mild hypoxia, and, together with its regulation of cellular damage in the ischaemic core, suggest a role for the antiporter in post‐ischaemic recovery of the penumbra.
Modeling Fatigue Failure of Cartilage and Fibrous Biological Tissues Using Constrained Reactive Mixture Theory
Authors: Zimmerman Brandon K. , Maas Steve A. , Weiss Jeffrey A. , Ateshian Gerard A.
Published: August 2024

Fatigue failure in biological soft tissues plays a critical role in the etiology of chronic soft tissue injuries and diseases such as osteoarthritis (OA). Understanding failure mechanisms is hindered by the decades-long timescales over which damage takes place. Analyzing the factors contributing to fatigue failure requires the help of validated computational models developed for soft tissues. This study presents a framework for fatigue failure of fibrous biological tissues based on reaction kinetics, where the composition of intact and fatigued material regions can evolve via degradation and breakage over time, in response to energy-based fatigue and damage criteria. Using reactive constrained mixture theory, material region mass fractions are governed by the axiom of mass balance. Progression of fatigue is controlled by an energy-based reaction rate, with user-selected probability functions defining the damage propensity of intact and fatigued material regions. Verification of this reactive theory, which is implemented in the open-source FEBio finite element software, is provided in this study. Validation is also demonstrated against experimental data, showing that predicted damage can be linked to results from biochemical assays. The framework is also applied to study fatigue failure during frictional contact of cartilage. Simulating previous experiments suggests that frictional effects slightly increase fatigue progression, but the main driver is cyclic compressive contact loading. This study demonstrated the ability of theoretical models to complement and extend experimental findings, advancing our understanding of the time progression of fatigue in biological tissues.
Sobolev-Type Nonlinear (k,ψ)−Hilfer Fractional Differential Equations With Control: Approximate Controllability Exploration
Authors: Mourad Kerboua , Ichrak Bouacida , Sami Segni
Published: August 2024

This paper is concerned with the approximate controllability of Sobolev-type (k,ψ)−Hilfer fractional differential equations (FDEs) with control and Sobolev-type (k,ψ)−Hilfer fractional initial conditions in Hilbert spaces. By means of two operators kSψα,β, kTψα and the k−probability density function, the definition of mild solutions for the studied problem was given. Then, via (k,ψ)−Hilfer fractional derivative and by combining the techniques of fractional calculus and the fixed point theorem, we analyzed the existence and uniqueness of mild solutions. With the help of a Cauchy sequence and approximate techniques, we established some sufficient conditions for the approximate controllability of the proposed control system. Finally, an example is presented for the demonstration of obtained results.