bluuebunny/crossref_metadata_2025 huggingface dataset:
Fat Distribution, Aerobic Fitness, Blood Lipids, and Insulin Sensitivity in African‐American and European‐American Women
Authors: Hunter Gary R. , Chandler‐Laney Paula C. , Brock David W. , Lara‐Castro Cristina , Fernandez Jose R. , Gower Barbara A.
Published: August 2009

The purpose of this study was to determine independent relationships of intra‐abdominal adipose tissue (IAAT), leg fat, and aerobic fitness with blood lipids and insulin sensitivity (Si) in European‐American (EA) and African‐American (AA) premenopausal women. Ninety‐three EA and ninety‐four AA with BMI between 27 and 30 kg/m2 had IAAT by computed tomography, total fat and leg fat by dual‐energy X‐ray absorptiometry, aerobic fitness by a graded exercise test, African admixture (AFADM) by ancestry informative markers, blood lipids by the Ektachem DT system, and Si by glucose tolerance test. Independent of age, aerobic fitness, AFADM, and leg fat, IAAT was positively related to low‐density lipoprotein–cholesterol (LDL‐C), cholesterol‐high‐density lipoprotein (HDL) ratio, triglycerides (TGs), and fasting insulin (standardized β varying 0.16–0.34) and negatively related to HDL‐cholesterol (HDL‐C) and Si (standardized β −0.15 and −0.25, respectively). In contrast, independent of age, aerobic fitness, AFADM, and IAAT, leg fat was negatively related to total cholesterol, LDL‐C, cholesterol‐HDL ratio, TGs, and fasting insulin (standardized β varying −0.15 to −0.21) and positively related to HDL‐C and Si (standardized β 0.16 and 0.23). Age was not independently related to worsening of any blood lipid but was related to increased Si (standardized β for Si 0.25, insulin −0.31). With the exception of total cholesterol and LDL‐C, aerobic fitness was independently related to worsened blood lipid profile and increased Si (standardized β varying 0.17 to −0.21). Maintenance of favorable fat distribution and aerobic fitness may be important strategies for healthy aging, at least in premenopausal EA and AA women.
Effectiveness of a Prebariatric Surgery Insurance‐required Weight Loss Regimen and Relation to Postsurgical Weight Loss
Authors: Ochner Christopher N. , Puma Lauren M. , Raevuori Anu , Teixeira Julio , Geliebter Allan
Published: August 2009

Most US insurance companies require patients to participate in a medically supervised weight loss regimen prior to bariatric surgery. However, the utility of this requirement has not been documented. Data was collected from 94 bariatric surgery patients who were required, and 59 patients who were not required, by their insurance company to participate in a presurgical weight loss regimen. Weight change in the required group, as well as group differences in weight change, was examined from 3 and 6 months presurgery to 1 week presurgery, and from 1 week presurgery to 3 months postsurgery. Weight change presurgery was then used to predict weight loss postsurgery. In the 6 months prior to surgery, required patients gained 3.7 kg ± 5.9 (s.d.) (P < 0.0005), which did not differ from nonrequired patients. From surgery to 3 months postsurgery, required patients lost 23.6 ± 8 kg (P < 0.0005), also without differing from nonrequired patients. Patients who gained more weight prior to surgery, lost more weight postsurgery (P = 0.001), while controlling for initial weight. Findings suggest that the common weight loss regimen requirements of US insurance carriers were ineffective in producing presurgical weight loss in this sample. Most patients (>70%) in this sample gained weight prior to surgery, potentially taking advantage of final opportunities to overindulge in preferred foods. Required patients fared no better in terms of weight change postsurgically and, surprisingly, presurgical weight gain predicted better postsurgical weight loss outcome. Several potential explanations for this finding are offered.
Water Consumption Increases Weight Loss During a Hypocaloric Diet Intervention in Middle‐aged and Older Adults
Authors: Dennis Elizabeth A. , Dengo Ana Laura , Comber Dana L. , Flack Kyle D. , Savla Jyoti , Davy Kevin P. , Davy Brenda M.
Published: August 2009

Water consumption acutely reduces meal energy intake (EI) among middle‐aged and older adults. Our objectives were to determine if premeal water consumption facilitates weight loss among overweight/obese middle‐aged and older adults, and to determine if the ability of premeal water consumption to reduce meal EI is sustained after a 12‐week period of increased water consumption. Adults (n = 48; 55–75 years, BMI 25–40 kg/m2) were assigned to one of two groups: (i) hypocaloric diet + 500 ml water prior to each daily meal (water group), or (ii) hypocaloric diet alone (nonwater group). At baseline and week 12, each participant underwent two ad libitum test meals: (i) no preload (NP), and (ii) 500 ml water preload (WP). Meal EI was assessed at each test meal and body weight was assessed weekly for 12 weeks. Weight loss was ∼2 kg greater in the water group than in the nonwater group, and the water group (β = −0.87, P < 0.001) showed a 44% greater decline in weight over the 12 weeks than the nonwater group (β = −0.60, P < 0.001). Test meal EI was lower in the WP than NP condition at baseline, but not at week 12 (baseline: WP 498 ± 25 kcal, NP 541 ± 27 kcal, P = 0.009; 12‐week: WP 480 ± 25 kcal, NP 506 ± 25 kcal, P = 0.069). Thus, when combined with a hypocaloric diet, consuming 500 ml water prior to each main meal leads to greater weight loss than a hypocaloric diet alone in middle‐aged and older adults. This may be due in part to an acute reduction in meal EI following water ingestion.
Hepatic Expression of Cell Death–inducing DFFA‐like Effector C in Obese Subjects Is Reduced by Marked Weight Loss
Authors: Hall Angela M. , Brunt Elizabeth M. , Klein Samuel , Finck Brian N.
Published: August 2009

The hepatic expression of the cell death–inducing DNA fragmentation factor A–like effector family (CIDEA, CIDEB, and CIDEC) genes is markedly upregulated in mouse models of obesity. We evaluated the expression of CIDE genes in liver of obese human subjects undergoing gastric bypass surgery (GBS), at the time of surgery and again 1 year later when subjects had lost 37.6 ± 1.4% of their initial body weight. At the time of GBS, the expression of CIDEA (r2 = 0.20, P = 0.04) and CIDEC (r2 = 0.32, P = 0.01) was strongly correlated with BMI, whereas CIDEB was not (r2 = 0.01, P = 0.81). One year after surgery, CIDEC expression had declined over 60% (P = 0.02), whereas CIDEA expression did not change (P = 0.20). These data demonstrate that, consistent with previous studies conducted in rodents, hepatic expression of CIDEA and CIDEC, but not CIDEB, is increased in obese humans. Moreover, the hepatic expression of CIDEC is downregulated by marked weight loss.
Exercise training reduces circulating adiponectin levels in patients with chronic heart failure
Authors: Van Berendoncks An M. , Beckers Paul , Hoymans Vicky Y. , Possemiers Nadine , Wuyts Floris L. , Vrints Christiaan J. , Conraads Viviane M.
Published: August 2009

High adiponectin concentrations have emerged as an independent risk factor of outcome in patients with CHF (chronic heart failure); however, modification of adiponectin in CHF patients has not been assessed to date. The aim of the present study was to investigate the effect of exercise training on adiponectin levels in CHF patients. A total of 80 patients with CHF due to systolic dysfunction were included. The effect of 4 months exercise training was studied in 46 patients, whereas the remaining 34 untrained CHF patients served as a sedentary control group. Circulating adiponectin concentrations, exercise capacity, anthropometric data and NT-proBNP (N-terminal pro-brain natriuretic peptide) levels were assessed. Adiponectin levels were significantly higher in CHF patients compared with healthy subjects [9.3 (7.1–16.1) and 4.9 (3.9–8.6) mg/l respectively; P=0.015]. Stratification of CHF patients according to tertiles of NT-proBNP revealed an increase in adiponectin with disease severity (P<0.0001). Exercise training reduced circulating adiponectin levels in CHF patients [10.7 (7.2–17.6) mg/l before training to 9.4 (5.9–14.8) mg/l after training; P=0.013], whereas no changes were observed in the sedentary CHF group [9.0 (7.0–13.5) mg/l before training and 10.1 (6.0–15.7) mg/l after a similar time interval]. A significant time×group interaction (P=0.008) was observed for the mean change in adiponectin between the trained and untrained CHF patients. Adiponectin concentrations were positively associated with NT-proBNP and HDL (high-density lipoprotein)-cholesterol and negatively correlated with BMI (body mass index), triacylglycerols and exercise capacity. In conclusion, circulating adiponectin concentrations are higher in CHF patients compared with healthy subjects and increase with disease severity. Exercise training for 4 months lowers circulating adiponectin levels.
Enhanced circulating retinol and non-esterified fatty acids in pregnancies complicated with intrauterine growth restriction
Authors: Ortega-Senovilla Henar , Alvino Gioia , Taricco Emanuela , Cetin Irene , Herrera Emilio
Published: August 2009

IUGR (intrauterine growth restriction) increases the incidence of perinatal complications and, although several placental transport functions have been shown to be altered in pregnancies complicated by IUGR, the mechanism behind it is not well understood. The aim of the present study was to investigate factors in maternal and cord blood plasma from normal and IUGR-complicated pregnancies associated with the body weight of newborns. At the time of Caesarean section, 24 women with IUGR pregnancies were compared with a group of 30 normal controls with AGA (appropriate gestational age) fetuses who were studied at Caesarean section, which took place 5 weeks later than IUGR pregnancies, and also to a group of 25 non-delivered gestational age-matched control pregnant women (AGA-35wk). Maternal plasma retinol, γ- and α-tocopherol, NEFAs (non-esterified fatty acids), and palmitic, palmitoleic, γ-linolenic and arachidonic acids were higher in women with IUGR pregnancies than in AGA-35wk controls, whereas stearic and α-linolenic acids were lower. Smaller differences were found when comparing these variables for IUGR and AGA women. However, umbilical vein plasma γ-tocopherol, cholesterol, triacylglycerols and NEFAs were higher in the IUGR group than in the AGA group, whereas arachidonic acid was lower. Maternal plasma retinol and NEFAs were the only variables negatively correlated with birthweight when multiple linear regressions were analysed. In conclusion, the increased levels of circulating retinol and NEFAs in maternal plasma are negatively associated with birth and placental weights, which may reflect an impaired placental transfer in IUGR pregnancies. As retinoids are involved in the control of gene transcription, it is proposed that a decrease in placental transfer of retinol could underlie the metabolic dysfunction of IUGR pregnancies.
Theorising Transitional Pathways in Response to Climate Change: Technocentrism, Ecocentrism, and the Carbon Economy
Authors: Bailey Ian , Wilson Geoff A
Published: August 2009

The carbon economy has emerged over the last decade as an important but controversial development in the harnessing of economics to address the challenge of climate change. In this paper we utilise the concepts of policy change and transition theorisations to analyse carbon commodification within the broader range of possible responses to the climate problem. We argue that the neoliberal, technocentric, and ecological modernisation values underpinning the carbon economy create serious obstacles for the incorporation of alternative or complementary transitional strategies, particularly those involving ecocentric changes, into mainstream policy. From this we contend that a more holistic approach to the conceptualisation of change processes is needed, and that transition theory provides a useful lens for identifying and assessing existing constraints and future possibilities.
Carbon Offsetting: Sustaining Consumption?
Authors: Lovell Heather , Bulkeley Harriet , Liverman Diana
Published: August 2009

In this paper we examine how theories of sustainable and ethical consumption help us to understand a new, rapidly expanding type of consumer product designed to mitigate climate change: carbon offsets. The voluntary carbon offset market grew by 200% between 2005 and 2006, and there are now over 150 retailers of voluntary carbon offsets worldwide. Our analysis concentrates on the production and consumption of carbon offsets, drawing on ideas from governmentality and political ecology about how narratives and technologies are used to create particular types of consumer subjectivities and shape consumer choice. We critically examine three narratives that offset producers are using to position carbon offsets and examine how these narratives are shaping circuits of carbon offset production and consumption. We assess the implications for the future governance of voluntary carbon offset markets and for the study of alternative consumption.
Climate Change and Hazardscape of Sri Lanka
Authors: Yamane Akiko
Published: August 2009

In recent years ‘vulnerability assessment’ has gained a prominent position in the international climate-change policy arena. There are many social-scientific studies that examine various methods and approaches involved in assessing vulnerability. Rather than making another addition to this literature I examine how climate-change policies have been translated in Sri Lanka in order to identify vulnerable places and social groups by combining actor-network theory and the concept of ‘hazardscape’. In so doing I illustrate how concepts of climate-change vulnerability and hazards are, more often than not, translated in ways that fit within and reinforce existing official narratives of social and spatial disadvantage, rather than addressing new problems or dimensions that might emerge from the combined effects of changes in climate, economy, and society. I conclude by suggesting that the concept of ‘resilience’ needs to be highlighted within international climate-change policy because it offers smaller developing countries the possibility of going beyond mitigation and adaptation strategies and finding innovative ways of integrating traditional resource-use insights and approaches within Clean Development Mechanism projects.
From Public to Private Global Environmental Governance: Lessons from the Montreal Protocol's Stalled Methyl Bromide Phase-Out
Authors: Gareau Brian J , DuPuis E Melanie
Published: August 2009

The Montreal Protocol on Substances that Deplete the Ozone Layer, a multilateral environmental agreement, has successfully eliminated the use of most ozone-depleting chlorofluoro-carbons. As a result, a number of observers have pointed to the possibility of transferring successes—and even linking regulations—between the Montreal Protocol and Kyoto Protocol, the international but stalled climate-change agreement. We argue that there is need for caution on this issue. The Montreal and Kyoto protocols are the outcomes of vastly different political contexts, from public civil society approaches to what we call ‘the private turn’: the current loss of faith in state sovereignty, the rejection of multilateralism, and an embrace of private knowledge about economic damage over public knowledge about the protection of citizens and natural resources. From this broader perspective we show that the differences between the Montreal and Kyoto protocols are therefore more than ‘command-and-control’ versus ‘market-based’ solutions. These differences also reflect an even deeper divide over what ‘counts’ as knowledge in political decision-making processes. We illustrate these points through a case study of the current knowledge controversies around the phase-out of methyl bromide under the Montreal Protocol. We explain how the methyl bromide phase-out has stalled because the phase-out approach is incompatible with the current political regime, thus supporting the argument that neoliberal forms of governance cannot solve global environmental problems. This case, therefore, shows us that the challenges we face are more than atmospheric: to save the Earth we must create new ways to govern ourselves.
The Influence of Viewpoint and Object Detail in Blind People When Matching Pictures to Complex Objects
Authors: Heller Morton A , Riddle Tara , Fulkerson Erin , Wemple Lindsay , Walk Anne McClure , Guthrie Stephanie , Kranz Crystal , Klaus Patricia
Published: August 2009

We examined haptic viewpoint effects in blindfolded-sighted (BS) and visually impaired subjects: early blind (EB), late blind (LB), and very low vision (VLV). Participants felt complex objects and matched tangible pictures to them. In experiment 1, the EB and BS subjects had similar overall performance. Experiment 2 showed that the presence of a detail on the target object lowered performance in the BS subjects, and that matching accuracy was lower overall for top views for the blind subjects. In experiments 3–5, EB, LB, VLV, and BS subjects made judgments about perspective pictures of a model house with more salient object details. In experiment 3, performance was higher for side views than for corner views. Elevated side views were identified more readily than elevated corner views in experiment 4. Performance for top views was higher than for elevated side views in experiment 5, given the relative simplicity of the top-view depictions and salient details. The EB and BS participants had somewhat lower matching accuracy scores than the other groups. We suggest that visual experience is helpful, but not essential for picture perception. Viewpoint effects may vary with experience and object complexity, but the relevant experience need not be specifically visual in nature.
A Significant Bilateral Field Advantage for Shapes Defined by Static and Motion Cues
Authors: Collin Charles A , McMullen Patricia A , Séguin Julie-Anne
Published: August 2009

Matching performance is better when pairs of visual stimuli are presented in bilateral conditions—in which one stimulus is presented to each side of the visual field—than in unilateral presentations—when both stimuli are presented to one side of the field. This is called the bilateral field advantage (BFA). The processing of visual motion has also been found to be more strongly integrated across the cerebral hemispheres than is processing of static cues. However, in these studies higher-order motion tasks, such as processing motion-defined form, have not been examined. To determine if the BFA generalises to such tasks, we measured the magnitude of the effect using a shape-matching task in which the stimuli were random polygons that were either in motion, motion-defined, or static. The polygon pairs were presented either: (i) bilaterally, one to either side of the vertical meridian; (ii) unilaterally, both to one side of the vertical meridian (left or right visual fields); or (iii) centrally, vertically separated across the horizontal meridian (a control condition). An equal advantage of bilateral conditions over unilateral ones was found for all three types of polygon shape cues, showing that the BFA generalises to conditions where shapes are in motion and where shape is defined by motion. These findings are compatible with the notion that motion processing is strongly integrated across the cerebral hemispheres, and with the idea that this integration manifests itself with simple motion information, rather than with higher-order motion processing such as matching shapes defined by motion.
Human vulnerability to stress depends on amygdala's predisposition and hippocampal plasticity
Authors: Admon Roee , Lubin Gad , Stern Orit , Rosenberg Keren , Sela Lee , Ben-Ami Haim , Hendler Talma
Published: August 2009

Variations in people's vulnerability to stressful life events may rise from a predated neural sensitivity as well as from differential neural modifications in response to the event. Because the occurrence of a stressful life event cannot be foreseen, characterizing the temporal trajectory of its neural manifestations in humans has been a real challenge. The current prospective study examined the emotional experience and brain responses of 50 a priori healthy new recruits to the Israeli Defense Forces at 2 time points: before they entered their mandatory military service and after their subsequent exposure to stressful events while deployed in combat units. Over time, soldiers reported on increase in stress symptoms that was correlated with greater amygdala and hippocampus responsiveness to stress-related content. However, these closely situated core limbic regions exhibited different temporal trajectories with regard to the stress effect; whereas amygdala's reactivity before stress predicted the increase in stress symptoms, the hippocampal change in activation over time correlated with the increase in such symptoms. Hippocampal plasticity was also reflected by a modification over time of its functional coupling with the ventromedial prefrontal cortex, and this coupling magnitude was again predicted by predated amygdala reactivity. Together, these findings suggest that variations in human's likelihood to develop symptomatic phenomena following stressful life events may depend on a balanced interplay between their amygdala's predisposing reactivity and hippocampal posteriori intra- and interregional plasticity. Accordingly, an individually tailored therapeutic approach for trauma survivors should target these 2 neural probes while considering their unique temporal prints.
Thermodynamic basis for the optimization of binding-induced biomolecular switches and structure-switching biosensors
Authors: Vallée-Bélisle Alexis , Ricci Francesco , Plaxco Kevin W.
Published: August 2009

Binding-induced biomolecular switches are used throughout nature and, increasingly, throughout biotechnology for the detection of chemical moieties and the subsequent transduction of this detection into useful outputs. Here we show that the thermodynamics of these switches are quantitatively described by a simple 3-state population-shift model, in which the equilibrium between a nonbinding, nonsignaling state and the binding-competent, signaling state is shifted toward the latter upon target binding. Because of this, their performance is determined by the tradeoff inherent to their switching thermodynamics; while a switching equilibrium constant favoring the nonbinding, nonsignaling, conformation ensures a larger signal change (more molecules are poised to respond), it also reduces affinity (binding must overcome a more unfavorable conformational free energy). We then derive and employ the relationship between switching thermodynamics and switch signaling to rationally tune the dynamic range and detection limit of a representative structure-switching biosensor, a molecular beacon, over 4 orders of magnitude. These findings demonstrate that the performance of biomolecular switches can be rationally tuned via mutations that alter their switching thermodynamics and suggest a mechanism by which the performance of naturally occurring switches may have evolved.
Variations in the colchicine-binding domain provide insight into the structural switch of tubulin
Authors: Dorléans Audrey , Gigant Benoît , Ravelli Raimond B. G. , Mailliet Patrick , Mikol Vincent , Knossow Marcel
Published: August 2009

Structural changes occur in the αβ-tubulin heterodimer during the microtubule assembly/disassembly cycle. Their most prominent feature is a transition from a straight, microtubular structure to a curved structure. There is a broad range of small molecule compounds that disturbs the microtubule cycle, a class of which targets the colchicine-binding site and prevents microtubule assembly. This class includes compounds with very different chemical structures, and it is presently unknown whether they prevent tubulin polymerization by the same mechanism. To address this issue, we have determined the structures of tubulin complexed with a set of such ligands and show that they interfere with several of the movements of tubulin subunits structural elements upon its transition from curved to straight. We also determined the structure of tubulin unliganded at the colchicine site; this reveals that a β-tubulin loop (termed T7) flips into this site. As with colchicine site ligands, this prevents a helix which is at the interface with α-tubulin from stacking onto a β-tubulin β sheet as in straight protofilaments. Whereas in the presence of these ligands the interference with microtubule assembly gets frozen, by flipping in and out the β-subunit T7 loop participates in a reversible way in the resistance to straightening that opposes microtubule assembly. Our results suggest that it thereby contributes to microtubule dynamic instability.
A Markov model of the Indus script
Authors: Rao Rajesh P. N. , Yadav Nisha , Vahia Mayank N. , Joglekar Hrishikesh , Adhikari R. , Mahadevan Iravatham
Published: August 2009

Although no historical information exists about the Indus civilization (flourishedca. 2600–1900 B.C.), archaeologists have uncovered about 3,800 short samples of a script that was used throughout the civilization. The script remains undeciphered, despite a large number of attempts and claimed decipherments over the past 80 years. Here, we propose the use of probabilistic models to analyze the structure of the Indus script. The goal is to reveal, through probabilistic analysis, syntactic patterns that could point the way to eventual decipherment. We illustrate the approach using a simple Markov chain model to capture sequential dependencies between signs in the Indus script. The trained model allows new sample texts to be generated, revealing recurring patterns of signs that could potentially form functional subunits of a possible underlying language. The model also provides a quantitative way of testing whether a particular string belongs to the putative language as captured by the Markov model. Application of this test to Indus seals found in Mesopotamia and other sites in West Asia reveals that the script may have been used to express different content in these regions. Finally, we show how missing, ambiguous, or unreadable signs on damaged objects can be filled in with most likely predictions from the model. Taken together, our results indicate that the Indus script exhibits rich synactic structure and the ability to represent diverse content. both of which are suggestive of a linguistic writing system rather than a nonlinguistic symbol system.
Salmonella regulates polyubiquitination and surface expression of MHC class II antigens
Authors: Lapaque Nicolas , Hutchinson James L. , Jones Des C. , Méresse Stéphane , Holden David W. , Trowsdale John , Kelly Adrian P.
Published: August 2009

Salmonella typhimuriumis a facultative pathogen capable of entering and replicating in both professional and non-professional antigen presenting cells. Control of infection requires MHC class II restricted CD4 T-helper cell responses. Here we show thatSalmonellainfection induced polyubiquitination of HLA-DR, a post-translational modification that led to removal of mature, peptide loaded, αβ dimers from the cell surface. Immature αβIi complexes were unaffected. Surface expression of all class II isotypes, HLA-DP, -DQ, and -DR, was reduced in infected cells, but other cell-surface molecules that traffic through class II peptide loading compartments were unaffected. ASalmonellastrain carrying a mutation inssaVdid not induce ubiquitination of class II, implicatingSalmonellaT3SS-2 effector proteins in the process. T3SS-2 effectors, with established or proposed roles in ubiquitination, were not required for class II down-regulation, suggesting that an additional T3SS-2 effector is involved in regulating MHC class II ubiquitination. Although recognized as a viral immune evasion strategy, here, we demonstrate that bacteria can control surface MHC expression through ubiquitination.
Embryonic arrest at midgestation and disruption of Notch signaling produced by the absence of both epsin 1 and epsin 2 in mice
Authors: Chen Hong , Ko Genevieve , Zatti Alessandra , Di Giacomo Giuseppina , Liu Lijuan , Raiteri Elisabetta , Perucco Ezio , Collesi Chiara , Min Wang , Zeiss Caroline , De Camilli Pietro , Cremona Ottavio
Published: August 2009

Epsins are endocytic adaptors with putative functions in general aspects of clathrin-mediated endocytosis as well as in the internalization of specific membrane proteins. We have now tested the role of the ubiquitously expressed epsin genes, Epn1 and Epn2, by a genetic approach in mice. While either gene is dispensable for life, their combined inactivation results in embryonic lethality at E9.5–E10, i.e., at the beginning of organogenesis. Consistent with studies inDrosophila, where epsin endocytic function was linked to Notch activation, developmental defects observed in epsin 1/2 double knockout (DKO) embryos recapitulated those produced by a global impairment of Notch signaling. Accordingly, expression of Notch primary target genes was severely reduced in DKO embryos. However, housekeeping forms of clathrin-mediated endocytosis were not impaired in cells derived from these embryos. These findings support a role of epsin as a specialized endocytic adaptor, with a critical role in the activation of Notch signaling in mammals.
Inhibited sound change
Authors: Blevins Juliette , Wedel Andrew
Published: August 2009

The study of regular sound change reveals numerous types of exceptionality. The type studied here has the profile of regular sound change, but appears to be inhibited where homophony would result. The most widely cited cases of this phenomenon are reviewed and new cases presented. If sound change can be inhibited by impending homophony, how is this to be represented and understood? Here we offer a model of variation-based sound change where category evolution incorporates lexical competition. Lexical Character Displacement predicts accentuation of differences among similar words when syntagmatic disambiguation is limited. In the cases under discussion, this accentuation inhibits merger. However, as we show, the same principle can inhibit sound change altogether, or give rise to extreme phonological contrasts under similar conditions.
What were the four divisions of Middle Chinese?
Authors: Ferlus Michel
Published: August 2009

Determining the nature of the four Divisions of the Qièyùn is a fundamental problem in the study of the phonetic history of Chinese. Analyses by Pulleyblank and Baxter make it possible to bring out two major changes from Old Chinese to Middle Chinese: a two-way split of the vowel system, and later the lenition of medial -r-. The use of models drawn from Mon-Khmer voice type register languages made it possible to reconstruct the phonetic bases of the four divisions. Div. I groups tense rimes, Div. II groups velarized rimes resulting from medial -r-, Div. III groups lax/breathy rimes. As for Div. IV, it represents rimes with the diphthong ie.
Pronominal borrowing among the Maya
Authors: Law Danny
Published: August 2009

A central concern in the study of language contact phenomena is the question of what linguistic features are more or less likely to be borrowed, and why. Pronominal borrowing, at least the direct borrowing of the phonological forms, is often ranked among the least common outcomes of language contact. This paper presents an extended case study of contact-induced changes in the system of person markers in several Mayan languages over nearly two thousand years of intense linguistic contact. The contact phenomena discussed appear to include the direct borrowing of pronominal ‘matter’, as well as the diffusion of structural and semantic ‘patterns’ that have led to a high degree of convergence in the overall system of pronominal reference in these languages. Possible social and linguistic motivations for the unusual contact-induced changes are considered.
Morphological dissociations in the L2 acquisition of an inflectionally rich language
Authors: Agathopoulou Eleni , Papadopoulou Despina
Published: August 2009

This study investigates the validity of Pinker’s (1991, 1999) Dual-Mechanism Model in the mental representation of regular and irregular active past perfective verbs in adult non-native Greek. In this model, regular inflection is computed by a symbolic rule, while irregular words are fully stored in the lexicon. A nonce-probe elicitation task showed that both natives and non-natives generalized the regular affix -s, and more so in regular than in irregular perfective verb stems. Moreover, the degree of similarity of the nonce verbs to real ones did not affect the affixation of regulars. Dissimilar irregulars were affixed less often than similar ones by the intermediate learners but neither by the advanced learners, nor by the natives. Our findings support computation for regulars, as proposed by the Dual Mechanism Model, both in native and in non-native language acquisition. Yet, the model’s claim for full storage of all irregular words is not verified.
Resources and obstacles in very advanced L2 French
Authors: Bartning Inge , Forsberg Lundell Fanny , Hancock Victorine
Published: August 2009

This study builds on the proposition that there are six developmental stages for spoken L2 French, based on morpho-syntactic criteria (Bartning and Schlyter 2004). In order to investigate developmental stages ‘beyond stage 6’, oral productions of several groups of advanced learners/users and native speakers are analyzed in terms of resources and obstacles. Among the resources, we investigate expected late features such as formulaic language and elaboration of information structure (Forsberg 2008; Hancock 2007). Morpho-syntactic deviances (MSDs), i.e. obstacles are also investigated. MSDs are expected to be almost absent beyond stage 6 (von Stutterheim 2003). Surprisingly, they continue to be present even at these very high levels. The results also show that formulaic language and information structure are promising measures of high levels, although the latter did not yield significant differences compared to lower stages. The study concludes by the proposal of a transitional stage with L2 users called functional bilinguals, which would constitute a stage between the advanced learner and the near-native speaker.
The effect of Study Abroad (SA) on written performance
Authors: Pérez-Vidal Carmen , Juan-Garau Maria
Published: August 2009

Research on the effects of Study Abroad (SA) periods on learners’ linguistic progress has tended to focus on oral skills, and few SA studies have focused on learners’ development in writing while abroad. The subjects in the present study were 37 advanced level non-native (NNSs) university students of English on a SA programme. Written compositions were analysed for fluency accuracy and complexity gains after the SA. They were contrasted with progress after formal instruction and with native speakers (NSs) baseline written performance. Language background data, attitudes, and stay abroad conditions were elicited with questionnaires. The SA period resulted in significant progress, which receded in the mid-term. Formal instruction only showed some improvement in accuracy. Significant differences were found between NSs and NNSs, although not in all domains. Results allow us to identify the students who benefit most from the SA and to examine the factors which seem to characterize them.
Function and Assembly of DNA Looping, Clustering, and Microtubule Attachment Complexes within a Eukaryotic Kinetochore
Authors: Anderson Marybeth , Haase Julian , Yeh Elaine , Bloom Kerry
Published: August 2009

The kinetochore is a complex protein–DNA assembly that provides the mechanical linkage between microtubules and the centromere DNA of each chromosome. Centromere DNA in all eukaryotes is wrapped around a unique nucleosome that contains the histone H3 variant CENP-A (Cse4p in Saccharomyces cerevisiae). Here, we report that the inner kinetochore complex (CBF3) is required for pericentric DNA looping at the Cse4p-containing nucleosome. DNA within the pericentric loop occupies a spatially confined area that is radially displaced from the interpolar central spindle. Microtubule-binding kinetochore complexes are not involved in pericentric DNA looping but are required for the geometric organization of DNA loops around the spindle microtubules in metaphase. Thus, the mitotic segregation apparatus is a composite structure composed of kinetochore and interpolar microtubules, the kinetochore, and organized pericentric DNA loops. The linkage of microtubule-binding to centromere DNA-looping complexes positions the pericentric chromatin loops and stabilizes the dynamic properties of individual kinetochore complexes in mitosis.
mTrs130 Is a Component of a Mammalian TRAPPII Complex, a Rab1 GEF That Binds to COPI-coated Vesicles
Authors: Yamasaki Akinori , Menon Shekar , Yu Sidney , Barrowman Jemima , Meerloo Timo , Oorschot Viola , Klumperman Judith , Satoh Ayano , Ferro-Novick Susan
Published: August 2009

The GTPase Rab1 regulates endoplasmic reticulum-Golgi and early Golgi traffic. The guanine nucleotide exchange factor (GEF) or factors that activate Rab1 at these stages of the secretory pathway are currently unknown. Trs130p is a subunit of the yeast TRAPPII (transport protein particle II) complex, a multisubunit tethering complex that is a GEF for the Rab1 homologue Ypt1p. Here, we show that mammalian Trs130 (mTrs130) is a component of an analogous TRAPP complex in mammalian cells, and we describe for the first time the role that this complex plays in membrane traffic. mTRAPPII is enriched on COPI (Coat Protein I)-coated vesicles and buds, but not Golgi cisternae, and it specifically activates Rab1. In addition, we find that mTRAPPII binds to γ1COP, a COPI coat adaptor subunit. The depletion of mTrs130 by short hairpin RNA leads to an increase of vesicles in the vicinity of the Golgi and the accumulation of cargo in an early Golgi compartment. We propose that mTRAPPII is a Rab1 GEF that tethers COPI-coated vesicles to early Golgi membranes.
Multi‐centre experience on the use of perhexiline in chronic heart failure and refractory angina: old drug, new hope
Authors: Phan Thanh Trung , Shivu Ganesh Nallur , Choudhury Anirban , Abozguia Khalid , Davies Chris , Naidoo Ulasini , Ahmed Ibrar , Yousef Zaheer , Horowitz John , Frenneaux Michael
Published: August 2009

Aims

The objective of this study is to report on our 5‐year collective experience on the use of perhexiline in the UK, in patients with chronic heart failure (CHF) and/or refractory angina with respect to ‘real‐life’ drug side effects and toxicity, therapeutic drug level monitoring, 5 year mortality outcomes and predictors of response to perhexiline therapy.

Methods and results

Data on clinical history, perhexiline monitoring, follow‐up, and mortality were retrospectively collated from centralized perhexiline databases from two tertiary referral centres. A total of 151 patients were on perhexiline therapy at two UK tertiary referral centres. At 3–4 months, 68.8% of patients had drug level within the therapeutic range and 20.8% were above the therapeutic range. A total of 58.9% of patients reported to have felt better on the perhexiline (responders). The presence of refractory angina was an independent predictor of response to perhexiline therapy (odds ratio 2.84, 95% confidence interval 1.28–6.32, P = 0.01). Five‐year mortality was non‐significantly different between patients with refractory angina, CHF, or both (20.5, 31.0, and 38.4%, P = 0.20, respectively).

Conclusion

Perhexiline therapy provides symptomatic relief in the majority of patients with minimal side effects or toxicity. Careful therapeutic level monitoring for dose titration is important to prevent acute and chronic toxicity. Patients with refractory angina were more likely to be responders.
Assessment of Lineages of Cercospora kikuchii in Louisiana for Aggressiveness and Screening Soybean Cultivars for Resistance to Cercospora Leaf Blight
Authors: Cai Guohong , Schneider Raymond W. , Padgett Guy B.
Published: August 2009

Cercospora leaf blight (CLB) became much more prevalent in Louisiana beginning in 1999. A previous study showed that the Louisiana population of Cercospora kikuchii, the causal agent, was dominated by a new lineage that differed from those collected in other locations at earlier times. In this study, we tested whether the dominance of the new lineage was caused by higher aggressiveness and screened soybean cultivars for resistance to CLB. Representative isolates from both lineages were used individually to inoculate six soybean cultivars in the greenhouse. Contrary to expectations, the new lineage was less aggressive. Three virulence groups were defined in this pathogen based on correlation of the aggressiveness of individual isolates on soybean cultivars. Other possible causes that led to the dominance of the new lineage in Louisiana are discussed. Eleven soybean cultivars were tested for disease reaction at two locations over 3 years in the field. Two cultivars, AG5701 (Asgrow) and TV59R85 (Terral), were among the more resistant cultivars to CLB both in the greenhouse and in the field.
Identification and Properties of a Carlavirus Causing Chlorotic Mottle of Florists' Hydrangea (H. macrophylla) in the United States
Authors: Machado Caballero Jose Ernesto , Lockhart Ben E. , Mason Shauna L. , Daughtrey Margery
Published: August 2009

A previously uncharacterized virus with flexuous filamentous particles 660 nm in length was identified in the United States in florists' hydrangea (Hydrangea macrophylla), in which it caused chlorotic mottling, leaf deformation, and discoloration. The virus, tentatively named Hydrangea chlorotic mottle virus (HdCMV), was transmitted readily by mechanical inoculation and by Myzus persicae, but infected only H. macrophylla. The amino acid sequence of a 1.7-kb amplicon comprising the 3′ terminus of the HdCMV genome contained one partial and three complete putative open reading frames (ORFs) most similar in size, arrangement, and sequence to the homologous regions of the genomes of known carlaviruses. Based on virion morphology, genome properties, and current criteria for species demarcation, it was concluded that HdCMV represented a new species in the genus Carlavirus. Hydrangea ringspot virus (HdRSV, genus Potexvirus) occurred in mixed infections with HdCMV, but the two viruses could be distinguished readily by serological tests.
Burkholderia glumae and B. gladioli Cause Bacterial Panicle Blight in Rice in the Southern United States
Authors: Nandakumar Rangaraj , Shahjahan A. K. M. , Yuan X. L. , Dickstein E. R. , Groth D. E. , Clark C. A. , Cartwright R. D. , Rush M. C.
Published: August 2009

Bacterial panicle blight (BPB) is among the three most limiting rice diseases in Louisiana and the southern United States. The identity and characterization of pathogens associated with this disease was unclear. This research details studies carried out on the pathogens causing BPB on rice in Louisiana and other rice producing southern states. Bacterial strains were isolated from BPB-infected sheath, panicle, or grain samples collected from rice fields in Louisiana, Arkansas, Texas, and Mississippi. In greenhouse inoculation tests, 292 of 364 strains were pathogenic on rice seedlings or panicles. Identification of strains in the pathogen complex by growth on S-PG medium, carbon source utilization profile (Biolog), cellular fatty acid analysis, and polymerase chain reaction (PCR) methods revealed that 76 and 5% of the strains were Burkholderia glumae and B. gladioli, respectively. The other strains have not been conclusively identified. Although strains of both species produced similar symptoms on rice, B. glumae strains were generally more aggressive and caused more severe symptoms on rice than B. gladioli. Virulent strains of both species produced toxoflavin in culture. The two species had similar growth responses to temperature, and optima ranged from 38 to 40°C for B. glumae and 35 to 37°C for B. gladioli. PCR was the most sensitive and accurate method tested for identifying the bacterial pathogens to the species level. The 16S rDNA gene and 16S-23S rDNA internal transcribed spacer (ITS) region sequences of the B. glumae and B. gladioli strains from rice showed more than 99% sequence homology with published sequences. A real-time PCR system was developed to detect and quantify this pathogen from infected seed lots. Our results clearly indicate that B. glumae and B. gladioli were the major pathogens causing BPB in the southern United States.
Geographic Distribution and rDNA-ITS Region Sequence Diversity of Waitea circinata var. circinata Isolated from Annual Bluegrass in the United States
Authors: Chen Chi-Min , de la Cerda Karla A. , Kaminski John E. , Douhan Greg W. , Wong Francis P.
Published: August 2009

Waitea circinata var. circinata is the causal agent of brown ring patch, an emergent disease of turfgrass in the United States. Forty-two isolates from annual bluegrass were obtained from California, Connecticut, Idaho, Illinois, Massachusetts, New York, Ohio, Oregon, and Rhode Island. Almost all isolates produced white to orange sclerotia (bulbils), 2 to 5 mm in size, that turned dark brown after 21 days on ¼-strength potato dextrose agar. The ribosomal DNA internal transcribed spacer regions and 5.8S region (ITS) were analyzed by restriction fragment length polymorphism (RFLP) analysis using MspI and sequencing to attempt identification of the isolates. Some isolates were heterozygous at the MspI restriction site, results not found in previous reports using the RFLP technique for identification. Four additional nucleotide positions were found to be variable within ITS based on sequence analysis, including two indels and two additional heterozygous positions. A total of 17 ITS haplotypes were found, and there was no obvious relationship between ITS haplotype and the geographic distribution of the isolates. Results of this work indicate that W. circinata var. circinata is present in multiple states and provide an initial understanding of the diversity of the pathogen in the United States.
Episodic Abiotic Stress as a Potential Contributing Factor to Onset and Severity of Disease Caused by Phytophthora ramorum in Rhododendron and Viburnum
Authors: Roubtsova Tatiana V. , Bostock Richard M.
Published: August 2009

Phytophthora ramorum attacks many forest and nursery species, primarily causing trunk or stem cankers, foliar blight, and dieback, and in some species root infection has been demonstrated. However, the abiotic and edaphic factors that influence infection and disease development are unresolved. Root infection by P. ramorum and the potential for mild abiotic stress in disease predisposition was examined with Rhododendron hybrid Cunningham's White and Viburnum tinus cv. Spring Bouquet. To impose water stress in a uniform and synchronous manner, osmotic stress induced with 0.2 M NaCl was selected. Roots were exposed to NaCl for 16 to 24 h in modified hydroponic culture or standard potting soil, removed from the NaCl, and then inoculated with zoospores. In the hydroponic regime, disease symptoms developed in Rhododendron and V. tinus plants within 1 week after inoculation of salt-stressed roots, whereas symptom development was delayed in nonstressed, inoculated plants. Microscopic examination of roots from both species revealed that their apices were covered with sporangia of P. ramorum. On potted Rhododendron plants inoculated by applying zoospores directly to the soil, stem lesions developed rapidly in salt-stressed plants, with death of the plant occurring within 4 weeks after inoculation. Nonstressed plants survived for 6 to 8 weeks before succumbing to disease, and symptom development in these plants was delayed by 1 to 2 weeks relative to the inoculated, salt-stressed plants. A postinfection episode of salt stress to inoculated roots in the hydroponic regime resulted in significantly faster development of stem lesions in Rhododendron relative to nonstressed, inoculated plants.