bluuebunny/crossref_metadata_2025 huggingface dataset:
Draft Genomes of Two Artocarpus Plants, Jackfruit (A. heterophyllus) and Breadfruit (A. altilis)
Authors: Sahu Sunil , Liu Min , Yssel Anna , Kariba Robert , Muthemba Samuel , Jiang Sanjie , Song Bo , Hendre Prasad , Muchugi Alice , Jamnadass Ramni , Kao Shu-Min , Featherston Jonathan , Zerega Nyree , Xu Xun , Yang Huanming , Van Deynze Allen , de Peer Yves , Liu Xin , Liu Huan
Published: December 2019

Two of the most economically important plants in the Artocarpus genus are jackfruit (A. heterophyllus Lam.) and breadfruit (A. altilis (Parkinson) Fosberg). Both species are long-lived trees that have been cultivated for thousands of years in their native regions. Today they are grown throughout tropical to subtropical areas as an important source of starch and other valuable nutrients. There are hundreds of breadfruit varieties that are native to Oceania, of which the most commonly distributed types are seedless triploids. Jackfruit is likely native to the Western Ghats of India and produces one of the largest tree-borne fruit structures (reaching up to 45 kg). To-date, there is limited genomic information for these two economically important species. Here, we generated 273 Gb and 227 Gb of raw data from jackfruit and breadfruit, respectively. The high-quality reads from jackfruit were assembled into 162,440 scaffolds totaling 982 Mb with 35,858 genes. Similarly, the breadfruit reads were assembled into 180,971 scaffolds totaling 833 Mb with 34,010 genes. A total of 2822 and 2034 expanded gene families were found in jackfruit and breadfruit, respectively, enriched in pathways including starch and sucrose metabolism, photosynthesis, and others. The copy number of several starch synthesis-related genes were found to be increased in jackfruit and breadfruit compared to closely-related species, and the tissue-specific expression might imply their sugar-rich and starch-rich characteristics. Overall, the publication of high-quality genomes for jackfruit and breadfruit provides information about their specific composition and the underlying genes involved in sugar and starch metabolism.
The Amazon Rainforest of Pre-Modern Literature: Ethics, Values, and Ideals from the Past for Our Future. With a Focus on Aristotle and Heinrich Kaufringer
Authors: Classen Albrecht
Published: December 2019

The tensions between the STEM fields and the Humanities are artificial and might be the result of nothing but political and financial competition. In essence, all scholars explore their topics in a critical fashion, relying on the principles of verification and falsification. Most important proves to be the notion of the laboratory, the storehouse of experiences, ideas, imagination, experiments. For that reason, here the metaphor of the Amazon rainforest is used to illustrate where the common denominators for scientists and scholars rest. Without that vast field of experiences from the past the future cannot be built. The focus here is based on the human condition and its reliance on ethical ideals as already developed by Aristotle. In fact, neither science nor humanities-based research are possible without ethics. Moreover, as illustrated by the case of one of the stories by Heinrich Kaufringer (ca. 1400), human conditions have always been precarious, contingent, puzzling, and fragile, especially if ethics do not inform the individual’s actions. Pre-modern literature is here identified as an ‘Amazon rainforest’ that only waits to be explored for future needs.
Animal-Assisted Therapy in the Residential Treatment of Dual Pathology
Authors: Monfort Montolio Miguel , Sancho-Pelluz Javier
Published: December 2019

Animal-assisted therapy (AAT) is a complementary intervention of therapy that has shown positive results in the treatment of various pathologies. This study assesses the viability of the implementation and the effectiveness of an AAT program in patients diagnosed with substance abuse disorder and associated mental disorders (dual pathology). For the study, a dynamic prospective cohort was used, consisting of 43 patients in residential treatment. The program consisted of 10 sessions with a duration of about 60 min, where data was collected in the 3rd, 6th and 10th sessions. The Life Skills Profile questionnaire (LSP) and the Barratt Impulsiveness Scale (BIS-11) were used for subsequent evaluation. Patients who participated in the program showed an improvement in daily skills, which favoured a better quality of life and decreased impulsiveness, enabling them to regain self-control. These results suggest that the dog can be a multi-sensory stimulus that captures attention, and improves motivation, cooperation and patient involvement in therapy. It was concluded that AAT can serve as an adjunctive therapy in the rehabilitation processes of people diagnosed with dual pathology.
Sustaining Sporting Destinations through Improving Tourists’ Mental and Physical Health in the Tourism Environment: The Case of Korea
Authors: Jeong Yunduk , Kim Suk-Kyu , Yu Jae-Gu
Published: December 2019

The purpose of this study was to explore structural relationships between emotional experiences, novelty seeking, tourist satisfaction, and destination loyalty in the context of active sport tourism. The study emphasizes the mediating effect tourist satisfaction has on the relationship between emotional experiences and destination loyalty. The validities and reliabilities of the measures used were examined through confirmatory factor analysis (CFA) and correlation analysis using 230 domestic and international participants who attended a marathon race as amateur athletes. Structural equation modeling analysis with maximum likelihood estimation was conducted to investigate relationships between study variables. Findings disclosed the positive impacts of (a) emotional experiences on tourist satisfaction and destination loyalty, (b) novelty seeking on tourist satisfaction, and (c) tourist satisfaction on destination loyalty, and demonstrated that (d) tourist satisfaction fully mediates the relationship between emotional experiences and destination loyalty. Based on its results, this study (a) indicates that emotional experiences play key roles in predicting tourist satisfaction and destination loyalty, (b) provides an example of the merits of the Destination Emotion Scale (DES) in a sport tourism setting, (c) implies that both emotional experiences and novelty seeking should be incorporated into tourist behavior models, and (d) contributes to tourism studies by exploring the mediating effect of tourist satisfaction on the relation between emotional experiences and destination loyalty. Thus, destination managers should manage gorgeous natural views and beautiful cityscapes, and organize various fun events, such as prize and ticket giveaway events, music performances, and charity campaigns for tourists during events.
Quantifying Land Use/Land Cover and Landscape Pattern Changes and Impacts on Ecosystem Services
Authors: Zhao Qingjian , Wen Zuomin , Chen Shulin , Ding Sheng , Zhang Minxin
Published: December 2019

Based on satellite remote sensing image, GIS and Fragstats, this study modeled and calculated the dynamic changes of land use, land cover and landscape patterns in Guizhou Province, China, and calculated the changes of ecosystem service values (ESVs). The impacts of the evolution of landscape patterns on the ESVs were analyzed, and reasonable policy recommendations were made. The findings are as follows: (1) In the past two decades, the area of cropland and grassland has decreased; the area of water bodies, urban and rural, industrial and mining, and residential areas has increased; the area of forestland has increased first and then decreased. (2) The two major types of landscapes, cropland and grassland, are clearly being replaced by two land types, forest land and water bodies. (3) Overall, the degree of landscape aggregation and adjacency has decreased, and the landscape heterogeneity has increased. (4) The total amount of ESV in 2000, 2008, 2013 and 2017 was 2574 × 108 Yuan RMB, 2605 × 108 Yuan RMB, 2618 × 108 Yuan RMB and 2612 × 108 Yuan RMB, respectively. The changes of landscape patterns had important impacts on the ESVs. In order to solve the problems caused by the increasingly prominent changes in the landscape patterns and improve the ESVs, it is necessary to rationally plan and allocate land resources, optimize the industrial structures, and develop effective regulatory policies.
Health Risk Modifiers of Exposure to Persistent Pollutants among Indigenous Peoples of Chukotka
Authors: Chashchin Valery , Kovshov Aleksandr A. , Thomassen Yngvar , Sorokina Tatiana , Gorbanev Sergey A. , Morgunov Boris , Gudkov Andrey B. , Chashchin Maksim , Sturlis Natalia V. , Trofimova Anna , Odland Jon Ø. , Nieboer Evert
Published: December 2019

The aim of the study was to assess temporal trends in health risks related to most common persistent contaminants, including polychlorinated biphenyls (PCBs), dichloro-diphenyl-trichloroethanes (DDTs), lead (Pb), as well as mercury (Hg) among indigenous peoples living in coastal areas of Chukotka in Arctic Russia. This is examined in relation to exposure pathways and a range of social and behavioral factors capable of modifying the exposure to these contaminants, including place of residence, income, traditional subsistence, alcohol consumption, and awareness of risk prevention. The primary exposure pathway for PCBs is shown to be the intake of traditional foods, which explained as much as 90% of the total health risk calculated employing established risk guidelines. Nearly 50% of past DDT-related health risks also appear to have been contributed by contaminated indoor surfaces involving commonly used DDT-containing insecticides. Individuals who practiced traditional activities are shown to have experienced a 4.4-fold higher risk of exposure to PCBs and a 1.3-fold higher risk for DDTs, Pb, and Hg. Low income, high consumption of marine mammal fat, alcohol consumption, and lack of awareness of health risk prevention are attributed to a further 2- to 6-fold increase in the risk of PCBs exposure. Low socioeconomic status enhances the health risks associated with exposure to the persistent contaminants examined.
Inequalities in Poverty and Income between Single Mothers and Fathers
Authors: Lu Yuan-Chiao , Walker Regine , Richard Patrick , Younis Mustafa
Published: December 2019

Background: The American family structure has changed in the past few decades due to a rise in the divorce rate and unmarried women with children. Research suggests a salary disparity between men and women, especially for those women after pregnancy. However, these studies were confined to individuals within traditional families, and there is a lack of information of income disparity and poverty status between single mothers and fathers. The current study explored the disparities in single-parent families based on the household income and the poverty status using a set of nationwide censor data. Methods: The current study used data from the 2011 and 2013 Panel Study of Income Dynamics (N = 1135). Multivariate regression models were used in the analysis. Results: The demographic characteristics of the weighted population showed that taxable income, total income, and poverty status were higher for single fathers than mothers, while non-work income was higher for single mothers than fathers. Single mothers were much more likely to be at the crisis category than single fathers. Multivariate analyses showed that gender, age, marital status, years of experience, and geographic region had effects on taxable income, and only gender, marital status, and region had effects on poverty status. Conclusions: The results suggest that vulnerable group of single mothers was acknowledged according to income and poverty status. Age, marital status, years of experience, and region would be the critical factors for predicting the income and poverty status for single parenthood.
Latent Tuberculosis Infection among Healthcare Students and Postgraduates in a Mediterranean Italian Area: What Correlation with Work Exposure?
Authors: Verso Maria Gabriella , Serra Nicola , Ciccarello Antonina , Romanin Benedetta , Di Carlo Paola
Published: December 2019

Background: Tuberculosis screening is part of the standard protocol for evaluating the risk of infection in healthcare workers. The aim of this study was to evaluate the prevalence of latent tuberculosis infection (LTBI) among students attending various healthcare profession degree courses and postgraduate medical courses at the School of Medicine of the University of Palermo, Italy, and assess the possible professional origin of infection. Methods: In total, 2946 students (2082 undergraduates and 864 postgraduates) took part in a screening program for LTBI between January 2014 to April 2019 using the tuberculin skin test (TST). Students with a positive TST result underwent a Quantiferon-TB test (QFT). Results: Among the 2082 undergraduates, 23 (1.1%) had a positive TST; the result was confirmed with QFT for 13 (0.62%) of them. Among the 864 postgraduate students, 24 (2.78%) had a positive TST and only 18 (2.08%) showed a positive QTF. Latent tuberculosis infections were significantly more frequent among postgraduates than undergraduates (2.08% > 0.62%, p < 0.0001). There was a higher number of subjects previously vaccinated for TB (18.87% > 0.24%, p < 0.0001), and of vaccinated subjects found positive for TST and QTF (66.67% > 7.69%, p = 0.001) in the postgraduate group. Conclusion: Latent TB is relatively low among medical school students in our geographic area. Nevertheless, this infectious disease must be regarded as a re-emerging biohazard for which preventive strategies are required to limit the risk of infection, especially among exposed workers.
Phthalate Esters and Their Potential Risk in PET Bottled Water Stored under Common Conditions
Authors: Xu Xiangqin , Zhou Gang , Lei Kun , LeBlanc Gerald A. , An Lihui
Published: December 2019

A great deal of attention has been paid lately to release of phthalate esters (PAEs) from polyethylene terephthalate (PET) bottles into PET bottled drinking water due to their potential endocrine-disrupting effects. Three kinds of PAEs, including diethyl phthalate (DEP), dimethyl phthalate (DMP) and dibutyl phthalate (DBP), were detected in 10 popular brands of PET bottles in Beijing, ranging from 101.97 μg/kg to 709.87 μg/kg. Meanwhile, six kinds of PAEs, including DEP, DMP, DBP, n-butyl benzyl phthalate (BBP), di-n-octyl phthalate (DOP) and di(2-ethylhexyl) phthalate (DEHP), were detected in PET bottled water, ranging from 0.19 μg/L to 0.98 μg/L, under an outdoor storage condition, while their concentrations ranged from 0.18 μg/L to 0.71 μg/L under an indoor storage condition. Furthermore, the concentrations of PAEs in brand D and E bottles were slightly increased when the storage time was prolonged. In addition, the concentrations of PAEs in commercial water contained in brand B and H bottles and pure water contained in brand E and G bottles were also slightly increased with the increase of storage temperature. Interestingly, DBP mainly contributed to the increased PAEs levels in simulation water. These results suggest that a part of the PAEs in PET bottled water originated from plastic bottles, which was related to the storage time and temperature. However, the PAEs in PET bottled water only pose a negligible risk to consumers if they follow the recommendations, such as storage at a common place (24 °C), away from sun and in a short period of time.
Diagnosing Gaps in the Development of Palliative and End-of-Life Care: A Qualitative Exploratory Study
Authors: Chan Helen Y. L. , Lee Diana T. F. , Woo Jean
Published: December 2019

A global report found that the quality of dying in Hong Kong lagged behind that of other high-income economies. This study aims to examine the service gaps by conducting a qualitative exploratory study from multiple stakeholders’ perspectives. Purposive and snowball sampling strategies were used to maximize variation in the sample. We interviewed 131 participants, including patients, family members, health care providers, administrators, lawyers, and policy makers. The situation analysis helped identify the facilitators and barriers at individual, organizational, and socio-cultural levels that affect service development. Findings showed that awareness on palliative and end-of-life care is growing, but the existing care is limited in terms of acceptability, coverage, variation in practices, continuity, and sustainability. A number of policy, economic, socio-cultural, environmental, and legal factors were also found to hinder service development. Findings of this study demonstrated that the development of palliative and end-of-life care services involved a paradigm shift relating to society as a whole. The overarching theme is to formulate a government-led policy framework. Furthermore, a public health approach has been advocated to create a supportive environment for service development.
The Dilemma of Road Safety in the Eastern Province of Saudi Arabia: Consequences and Prevention Strategies
Authors: Jamal Arshad , Rahman Muhammad Tauhidur , Al-Ahmadi Hassan M. , Mansoor Umer
Published: December 2019

Road traffic crashes (RTCs) are one of the most critical public health problems worldwide. The WHO Global Status Report on Road Safety suggests that the annual fatality rate (per 100,000 people) due to RTCs in the Kingdom of Saudi Arabia (KSA) has increased from 17.4 to 27.4 over the last decade, which is an alarming situation. This paper presents an overview of RTCs in the Eastern Province, KSA, from 2009 to 2016. Key descriptive statistics for spatial and temporal distribution of crashes are presented. Statistics from the present study suggest that the year 2012 witnessed the highest number of crashes, and that the region Al-Ahsa had a significantly higher proportion of total crashes. It was concluded that the fatality rate for the province was 25.6, and the mean accident to injury ratio was 8:4. These numbers are substantially higher compared to developed countries and the neighboring Gulf states. Spatial distribution of crashes indicated that a large proportion of severe crashes occurred outside the city centers along urban highways. Logistic regression models were developed to predict crash severity. Model estimation analysis revealed that crash severity can be attributed to several significant factors including driver attributes (such as sleep, distraction, overspeeding), crash characteristics (such as sudden deviation from the lane, or collisions with other moving vehicles, road fences, pedestrians, or motorcyclists), and rainy weather conditions. After critical analysis of existing safety and infrastructure situations, various suitable crash prevention and mitigation strategies, for example, traffic enforcement, traffic calming measures, safety education programs, and coordination of key stakeholders, have been proposed.
Estradiol Metabolism: Crossroads in Pulmonary Arterial Hypertension
Authors: Tofovic Stevan P. , Jackson Edwin K.
Published: December 2019

Pulmonary arterial hypertension (PAH) is a debilitating and progressive disease that predominantly develops in women. Over the past 15 years, cumulating evidence has pointed toward dysregulated metabolism of sex hormones in animal models and patients with PAH. 17β-estradiol (E2) is metabolized at positions C2, C4, and C16, which leads to the formation of metabolites with different biological/estrogenic activity. Since the first report that 2-methoxyestradiol, a major non-estrogenic metabolite of E2, attenuates the development and progression of experimental pulmonary hypertension (PH), it has become increasingly clear that E2, E2 precursors, and E2 metabolites exhibit both protective and detrimental effects in PH. Furthermore, both experimental and clinical data suggest that E2 has divergent effects in the pulmonary vasculature versus right ventricle (estrogen paradox in PAH). The estrogen paradox is of significant clinical relevance for understanding the development, progression, and prognosis of PAH. This review updates experimental and clinical findings and provides insights into: (1) the potential impacts that pathways of estradiol metabolism (EMet) may have in PAH; (2) the beneficial and adverse effects of estrogens and their precursors/metabolites in experimental PH and human PAH; (3) the co-morbidities and pathological conditions that may alter EMet and influence the development/progression of PAH; (4) the relevance of the intracrinology of sex hormones to vascular remodeling in PAH; and (5) the advantages/disadvantages of different approaches to modulate EMet in PAH. Finally, we propose the three-tier-estrogen effects in PAH concept, which may offer reconciliation of the opposing effects of E2 in PAH and may provide a better understanding of the complex mechanisms by which EMet affects the pulmonary circulation–right ventricular interaction in PAH.
HB-EGF Improves the Hair Regenerative Potential of Adipose-Derived Stem Cells via ROS Generation and Hck Phosphorylation
Authors: Choi Nahyun , Kim Won-Serk , Oh Sang Ho , Sung Jong-Hyuk
Published: December 2019

Although adipose-derived stem cells (ASCs) have hair regenerative potential, their hair inductive capabilities are limited. The mitogenic and hair inductive effects of heparin binding-epidermal growth factor-like growth factor (HB-EGF) on ASCs were investigated in this study and the underlying mechanism of stimulation was examined. Cell growth, migration, and self-renewal assays, as well as quantitative polymerase chain reactions and immunostaining, were carried out. Telogen-to-anagen transition and organ culture using vibrissa follicles were also conducted. HB-EGF significantly increased ASC motility, including cell proliferation, migration, and self-renewal activity. The preconditioning of ASCs with HB-EGF induced telogen-to-anagen transition more rapidly in vivo, and injected PKH26-ASCs survived for longer periods of time. Conditioned medium obtained from HB-EGF-treated ASCs promoted hair growth in vivo, upregulating growth factors. In particular, thrombopoietin (THPO) also induced hair growth in vivo, stimulating dermal papilla cells (DPCs). Reactive oxygen species (ROS) appeared to play a key role in ASC stimulation as the inhibition of ROS generation and NOX4 knockout attenuated ASC stimulation and THPO upregulation by HB-EGF. In addition, the Hck phosphorylation pathway mediated the stimulation of ASCs by HB-EGF. In summary, HB-EGF increased the motility and paracrine effects of ASCs releasing THPO growth factor and THPO promoted hair growth-stimulating DPCs. ROS generation and Hck phosphorylation are key factors in HB-EGF-induced ASC stimulation. Therefore, combination therapy involving HB-EGF and ASCs may provide a novel solution for hair-loss treatment.
Deep-Fried Atractylodis Rhizoma Protects against Spleen Deficiency-Induced Diarrhea through Regulating Intestinal Inflammatory Response and Gut Microbiota
Authors: Shi Kun , Qu Linghang , Lin Xiong , Xie Ying , Tu Jiyuan , Liu Xianqiong , Zhou Zhongshi , Cao Guosheng , Li Shuiqing , Liu Yanju
Published: December 2019

According to the theories of traditional Chinese medicine, spleen deficiency often leads to diarrhea, and deep-fried Atractylodis Rhizoma (DAR) is commonly used for the treatment. However, the association between spleen deficiency and diarrhea remains unclear. The present study aimed to investigate the therapeutic effect of DAR for the treatment of diarrhea caused by spleen deficiency and analyze the related mechanisms. It was found that a high dose group of an ethanolic extract of deep-fried Atractylodis Rhizoma (EEDAR-H) significantly inhibited weight loss, diarrhea, and pathological changes in colon tissue induced by rhubarb. EEDAR-H was found to significantly reduce the level of intestinal inflammatory cytokines and increase the expression of gastrointestinal motility hormones. In addition, EEDAR-H significantly increased the expression of aquaporin 3 (AQP3) and aquaporin 8 (AQP8) and restored abnormal water metabolism; Shen-Ling-Bai-Zhu-San (SLBZS) induced the same effect as EEDAR-H. Additional tests on the mechanism found that EEDAR-H and SLBZS promoted the integrity of the intestinal barrier. Both significantly increased the expression of the tight junction protein ZO-1 and Occludin, inhibited the phosphorylation of p38MAPK and MLC, and significantly reduced the expression levels of PAR-2. Analysis of the gut microbiota indicated that overall changes in its structure were reversed after treatment with EEDAR-H or SLBZS, in addition to significant modulation of the abundance of different phyla. At the genus level, EEDAR-H or SLBZS significantly reduced the levels of potential pathogens and increased those of beneficial bacteria.
The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms
Authors: Porubiaková Otília , Bohálová Natália , Inga Alberto , Vadovičová Natália , Coufal Jan , Fojta Miroslav , Brázda Václav
Published: December 2019

p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53α isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53α isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53α isoforms.
TLQP-21, A VGF-Derived Peptide Endowed of Endocrine and Extraendocrine Properties: Focus on In Vitro Calcium Signaling
Authors: Bresciani Elena , Possenti Roberta , Coco Silvia , Rizzi Laura , Meanti Ramona , Molteni Laura , Locatelli Vittorio , Torsello Antonio
Published: December 2019

VGF gene encodes for a neuropeptide precursor of 68 kDa composed by 615 (human) and 617 (rat, mice) residues, expressed prevalently in the central nervous system (CNS), but also in the peripheral nervous system (PNS) and in various endocrine cells. This precursor undergoes proteolytic cleavage, generating a family of peptides different in length and biological activity. Among them, TLQP-21, a peptide of 21 amino acids, has been widely investigated for its relevant endocrine and extraendocrine activities. The complement complement C3a receptor-1 (C3aR1) has been suggested as the TLQP-21 receptor and, in different cell lines, its activation by TLQP-21 induces an increase of intracellular Ca2+. This effect relies both on Ca2+ release from the endoplasmic reticulum (ER) and extracellular Ca2+ entry. The latter depends on stromal interaction molecules (STIM)-Orai1 interaction or transient receptor potential channel (TRPC) involvement. After Ca2+ entry, the activation of outward K+-Ca2+-dependent currents, mainly the KCa3.1 currents, provides a membrane polarizing influence which offset the depolarizing action of Ca2+ elevation and indirectly maintains the driving force for optimal Ca2+ increase in the cytosol. In this review, we address the main endocrine and extraendocrine actions displayed by TLQP-21, highlighting recent findings on its mechanism of action and its potential in different pathological conditions.
Systems and Synthetic microRNA Biology: From Biogenesis to Disease Pathogenesis
Authors: Matsuyama Hironori , Suzuki Hiroshi I.
Published: December 2019

MicroRNAs (miRNAs) are approximately 22-nucleotide-long, small non-coding RNAs that post-transcriptionally regulate gene expression. The biogenesis of miRNAs involves multiple steps, including the transcription of primary miRNAs (pri-miRNAs), nuclear Drosha-mediated processing, cytoplasmic Dicer-mediated processing, and loading onto Argonaute (Ago) proteins. Further, miRNAs control diverse biological and pathological processes via the silencing of target mRNAs. This review summarizes recent findings regarding the quantitative aspects of miRNA homeostasis, including Drosha-mediated pri-miRNA processing, Ago-mediated asymmetric miRNA strand selection, and modifications of miRNA pathway components, as well as the roles of RNA modifications (epitranscriptomics), epigenetics, transcription factor circuits, and super-enhancers in miRNA regulation. These recent advances have facilitated a system-level understanding of miRNA networks, as well as the improvement of RNAi performance for both gene-specific targeting and genome-wide screening. The comprehensive understanding and modeling of miRNA biogenesis and function have been applied to the design of synthetic gene circuits. In addition, the relationships between miRNA genes and super-enhancers provide the molecular basis for the highly biased cell type-specific expression patterns of miRNAs and the evolution of miRNA–target connections, while highlighting the importance of alterations of super-enhancer-associated miRNAs in a variety of human diseases.
Dietary Polyphenols and Gene Expression in Molecular Pathways Associated with Type 2 Diabetes Mellitus: A Review
Authors: Kang Gideon Gatluak , Francis Nidhish , Hill Rodney , Waters Daniel , Blanchard Christopher , Santhakumar Abishek Bommannan
Published: December 2019

Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder with various contributing factors including genetics, epigenetics, environment and lifestyle such as diet. The hallmarks of T2DM are insulin deficiency (also referred to as β-cell dysfunction) and insulin resistance. Robust evidence suggests that the major mechanism driving impaired β-cell function and insulin signalling is through the action of intracellular reactive oxygen species (ROS)-induced stress. Chronic high blood glucose (hyperglycaemia) and hyperlipidaemia appear to be the primary activators of these pathways. Reactive oxygen species can disrupt intracellular signalling pathways, thereby dysregulating the expression of genes associated with insulin secretion and signalling. Plant-based diets, containing phenolic compounds, have been shown to exhibit remedial benefits by ameliorating insulin secretion and insulin resistance. The literature also provides evidence that polyphenol-rich diets can modulate the expression of genes involved in insulin secretion, insulin signalling, and liver gluconeogenesis pathways. However, whether various polyphenols and phenolic compounds can target specific cellular signalling pathways involved in the pathogenesis of T2DM has not been elucidated. This review aims to evaluate the modulating effects of various polyphenols and phenolic compounds on genes involved in cellular signalling pathways (both in vitro and in vivo from human, animal and cell models) leading to the pathogenesis of T2DM.
Modeling Resilience to Damage in Multiple Sclerosis: Plasticity Meets Connectivity
Authors: Stampanoni Bassi Mario , Iezzi Ennio , Pavone Luigi , Mandolesi Georgia , Musella Alessandra , Gentile Antonietta , Gilio Luana , Centonze Diego , Buttari Fabio
Published: December 2019

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelinating white matter lesions and neurodegeneration, with a variable clinical course. Brain network architecture provides efficient information processing and resilience to damage. The peculiar organization characterized by a low number of highly connected nodes (hubs) confers high resistance to random damage. Anti-homeostatic synaptic plasticity, in particular long-term potentiation (LTP), represents one of the main physiological mechanisms underlying clinical recovery after brain damage. Different types of synaptic plasticity, including both anti-homeostatic and homeostatic mechanisms (synaptic scaling), contribute to shape brain networks. In MS, altered synaptic functioning induced by inflammatory mediators may represent a further cause of brain network collapse in addition to demyelination and grey matter atrophy. We propose that impaired LTP expression and pathologically enhanced upscaling may contribute to disrupting brain network topology in MS, weakening resilience to damage and negatively influencing the disease course.
Rainfall Estimation from Tropospheric Attenuation Affecting Satellite Links
Authors: Giro Riccardo Angelo , Luini Lorenzo , Riva Carlo Giuseppe
Published: December 2019

A novel methodology for estimating rainfall rate from satellite signals is presented. The proposed inversion algorithm yields rain rate estimates by making opportunistic use of the downlink signal and exploiting local ancillary meteorological information (0 °C isotherm height and monthly convectivity index), which can be extracted on a Global basis from Numerical Weather Prediction (NWP) products. The methodology includes different expressions to take the different impact of stratiform and convective rain events on the link into due account. The model accuracy in predicting the rain rate is assessed (and compared to the one of other models), both on a statistical and on an instantaneous basis, by exploiting a full year of data collected in Milan, in the framework of the Alphasat Aldo Paraboni propagation experiment.
Exercise-Based Interventions in Hemodialysis Patients: A Systematic Review with a Meta-Analysis of Randomized Controlled Trials
Authors: Bogataj Špela , Pajek Maja , Pajek Jernej , Buturović Ponikvar Jadranka , Paravlic Armin H.
Published: December 2019

There is a lack of agreement on the efficacy of different exercise interventions in hemodialysis patients. We analyzed which exercise type is the most beneficial in terms of functional fitness and inflammation. A literature search of seven databases yielded 33 studies that met the inclusion criteria. Compared with an inactive control, the intervention group showed moderate effects (ES = 0.74; 95% CI 0.35 to 1.14; p < 0.001; and ES = 0.70; 95% CI 0.39 to 1.01; p < 0.001; respectively) on functional capacity (six-minute walk test) and oxygen consumption. Small nonsignificant effects were observed for aerobic (ES = −0.36; 95% CI −0.85 to 0.13; p = 0.154) and resistance (ES = −0.44; 95% CI −1.07 to 0.19; p = 0.169) training types, whereas moderate effects were found for combined (ES = −0.69; 95% CI −1.47 to 0.10; p = 0.088) training type based on a 10-repetition sit-to-stand test. Further, large and small effects were observed for aerobic (ES = −1.21; 95% CI −1.94 to −0.49; p = 0.001) and resistance training (ES = −0.54; 95% CI −0.90 to −0.17; p = 0.004) types on C-reactive protein. Overall, the results showed the numerically largest effect sizes for combined types compared to solely aerobic or resistance training types, with the differences between training types not reaching statistical significance. There was a significant modifying impact of age, training frequency, and session duration on performance and inflammatory outcomes.
Inflammatory Profile and Osteogenic Potential of Fracture Haematoma in Humans
Authors: Pountos Ippokratis , Walters Gavin , Panteli Michalis , Einhorn Thomas A. , Giannoudis Peter V.
Published: December 2019

Fracture haematoma forms immediately after fracture and is considered essential for the bone healing process. Its molecular composition has been briefly investigated with our current understanding being based on animal studies. This study aims to analyse the inflammatory cytokine content of fracture haematoma in humans and determine its effect on osteoprogenitor cells. Twenty-three patients were recruited following informed consent. Peripheral blood, fracture haematoma and bone were collected. A Luminex assay on the levels of 34 cytokines was performed and autologous peripheral blood samples served as control. Mesenchymal Stem Cells (MSCs) were isolated following collagenase digestion and functional assays were performed. Gene expression analysis of 84 key osteogenic molecules was performed. Thirty-three inflammatory cytokines were found to be significantly raised in fracture haematoma when compared to peripheral serum (p < 0.05). Amongst the most raised molecules were IL-8, IL-11 and MMP1, -2 and -3. Fracture haematoma did not significantly affect MSC proliferation, but ALP activity and calcium deposition were significantly increased in the MSCs undergoing osteogenic differentiation. Medium supplementations with fracture haematoma resulted in a statistically significant upregulation of osteogenic genes including the EGF, FGF2 and VEGFA. This seems to be the pathway involved in the osteogenic effect of fracture haematoma on bone cells. In conclusion, fracture haematoma is found to be a medium rich in inflammatory and immunomodulatory mediators. At the same time, it contains high levels of anti-inflammatory molecules, regulates osteoclastogenesis, induces angiogenesis and the production of the extracellular matrix. It appears that fracture haematoma does not affect osteoprogenitor cells proliferation as previously thought, but induces an osteogenic phenotype.
The Indo-Pacific Sergeant Abudefduf vaigiensis (Quoy & Gaimard, 1825) (Perciformes: Pomacentridae) in Libya, South-Central Mediterranean Sea
Authors: Osca David , Tanduo Valentina , Tiralongo Francesco , Giovos Ioannis , Almabruk Sara A. A. , Crocetta Fabio , Rizgalla Jamila
Published: December 2019

The Indo-Pacific Sergeant Abudefduf vaigiensis (Quoy & Gaimard, 1825) (Chordata: Pisces: Actinopterygii: Perciformes: Pomacentridae) is first recorded in the south-central Mediterranean Sea (Libya), based on the external morphology and the barcoding of a fragment of the cytochrome c oxidase subunit I gene. Present sightings from field surveys and social media include juveniles, sub-adults, and a single adult specimen, suggesting that the species is now established in Libyan waters. No certainties occur regarding timing and possible pathway of arrival of this species in the area, and it may have simply gone undetected for years. The joint effort of field studies and citizen science projects in collaboration with international organizations continues shedding light on bioinvasions in Libya, with valuable outcomes for the Mediterranean marine biology as a whole.
Determinants of Land Use/Cover Change in the Iberian Peninsula (1990–2012) at Municipal Level
Authors: Fernández-Nogueira David , Corbelle-Rico Eduardo
Published: December 2019

This work analyzes the determinants associated with main land use/cover changes in the Iberian Peninsula during the 1990–2012 period using a decision tree model. Our main objective is to identify broad-scale patterns that associate the characteristics of geographic areas with the dominant land use/cover change process based on CORINE Land Cover (Coordination of Information on the Environment) and defined in a previously published work. Biophysical, structural and socioeconomic variables were considered as potentially explanatory of the dominant change process at municipal scale. The resulting model allowed identification of a common pattern in Portugal and Spain (urbanization being highly associated to areas already densely populated in the previous period), but also some diverging ones. In particular, dominant trends in Portuguese territory appear to be highly determined by wildfire occurrence. In contrast, Spanish municipalities showed more diverse patterns, usually associated to biophysical determinants like average forest productivity or average terrain slope.
Facile Preparation of Porous Microfiber from Poly-3-(R)-Hydroxybutyrate and Its Application
Authors: Kundrat Vojtech , Matouskova Petra , Marova Ivana
Published: December 2019

In this study, we described the development of a simplified wet spinning method of the production of a novel type of porous continuous fiber based on poly-3-(R)-hydroxybutyrate (PHB). The principle of this method is precipitation of PHB dissolved in chloroform solution into the ethanol precipitation bath. The influence of various PHB concentrations and feed rates on specific surface area (measured by nitrogen absorption method) was studied. Materials were also characterized by SEM. Surface areas of fibers achieved by wet spinning were in the range of tens of m2.g−1, and the biggest surface area value was 55 m2.g–1. The average diameter of fibers was in the range of 20–120 μm and was dependent on both PHB concentration and feed rate. Optimum conditions for reaching stable fibers of high surface area were 3–5 % w.t. of PHB and feed rate 0.5–3 ml.h−1. Fibers were functionalized by adsorption of some natural plant extracts. The incorporation of active substances into fibers was confirmed by infrared spectroscopy. High antioxidant and antimicrobial effect of PHB-fibers with cloves extract was found, as well as excellent long-term stability and optimal dynamics of the release of active compounds. The newly produced material would be applicable in pharmacy, cosmetics, and wound healing.
Use of Selected Carbon Nanoparticles as Melittin Carriers for MCF-7 and MDA-MB-231 Human Breast Cancer Cells
Authors: Daniluk Karolina , Kutwin Marta , Grodzik Marta , Wierzbicki Mateusz , Strojny Barbara , Szczepaniak Jarosław , Bałaban Jaśmina , Sosnowska Malwina , Chwalibog Andre , Sawosz Ewa , Jaworski Sławomir
Published: December 2019

Despite advanced techniques in medicine, breast cancer caused the deaths of 627,000 women in 2018. Melittin, the main component of bee venom, has lytic properties for many types of cells, including cancer cells. To increase its toxic effect, carbon nanoparticles, graphene oxide, pristine graphene, and diamond were used as carriers of melittin to breast cancer cells. To date, the effects of carbon nanoparticles as carriers of melittin on cancer cells have not been studied. The present study was carried out on MCF-7 and MDA-MB-231 cell lines. The investigation consisted of structural analysis of complexes using transmission electron microscopy, zeta potential measurements, evaluation of cell morphology, assessment of cell viability and membrane integrity, investigation of reactive oxygen species production, and investigation of mitochondrial membrane potential. Cell death was examined by flow cytometry and a membrane test for 43 apoptotic proteins. The results indicate that melittin complex with nanographene oxide has a stronger toxic effect on breast cancer cells than melittin alone. Moreover, nanodiamonds can protect cells against the lytic effects of melittin. All complexes reduced, but not completely eliminated the level of necrosis, compared to melittin. Thus, results suggest that the use of carbon nanoparticles as carriers for melittin may find use in medicine in the future.
Artificial Dense Lattices of Magnetic Skyrmions
Authors: Sapozhnikov Maksim V. , Petrov Yuri V. , Gusev Nikita S. , Temiryazev Alexey G. , Ermolaeva Olga L. , Mironov Victor L. , Udalov Oleg G.
Published: December 2019

Multilayer Co/Pt films with perpendicular magnetic anisotropy are irradiated by focused a He+ ion beam to locally reduce the anisotropy value. The irradiated spots with the diameters of 100 and 200 nm are arranged in square lattices with the periods of 200 and 300 nm. The formation of nonuniform magnetic states within the spots was observed by magnetic force microscopy methods. We use the concentric distribution of the irradiation fluence within the spot to obtain the radial modulation of the anisotropy constant. This allows us to induce magnetic skyrmions during magnetization reversal of the system. The skyrmions remained stable at zero external magnetic field at room temperature. Magnetization hysteresis loops of the samples were investigated by magnetooptical methods and the results are in good agreement with micromagnetic simulations.
Study on Low-Temperature Emission Performance of Scandate Cathode with Micro-Blade-Type Arrays
Authors: Lu Zhipeng , Yin Shengyi , Zhang Zhaochuan , Ren Feng , Lv Xinping
Published: December 2019

In order to meet the requirements of high-frequency vacuum electronic devices with small size, high current density, and low working temperature, a kind of porous tungsten scandate cathode with micro-blade-type arrays was developed. The micro-blade-type arrays were fabricated by laser engraving technology. Subsequently, the cathode was prepared by a vacuum copper removal process and impregnated with active substances at high temperature. Experimental results show that the cathode exhibits excellent low-temperature electron emission performance and that the maximum pulse electron emission current density reaches 81.18 A/cm2 at 800 °C. The cathode also shows apparent combined thermal-field emission characteristics. Further analysis shows that a high electric field strength plays an important role in the electron emission of the scandate cathode. By virtue of the electric field enhancement effect formed by the fabricated micro-blade-type arrays on the cathode surface, the prepared cathode achieves high electron emission capacity.
Densification of Magnesium Aluminate Spinel Using Manganese and Cobalt Fluoride as Sintering Aids
Authors: Talimian Ali , Pouchly Vaclav , Maca Karel , Galusek Dusan
Published: December 2019

Highly dense magnesium aluminate spinel bodies are usually fabricated using pressure-assisted methods, such as spark plasma sintering (SPS), in the presence of lithium fluoride as a sintering aid. The present work investigates whether the addition of transition metal fluorides promotes the sintering of MgAl2O4 bodies during SPS. At the same time, such fluorides can act as a source of optically active dopants. A commercial MgAl2O4 was mixed with 0.5 wt% of LiF, MnF2, and CoF2 and, afterwards, consolidated using SPS at 1400 °C. Although MnF2 and CoF2 promote the densification as effectively as LiF, they cause significant grain growth.
Properties of Undoped Few-Layer Graphene-Based Transparent Heaters
Authors: Zhang Yong , Liu Hao , Tan Longwang , Zhang Yan , Jeppson Kjell , Wei Bin , Liu Johan
Published: December 2019

In many applications like sensors, displays, and defoggers, there is a need for transparent and efficient heater elements produced at low cost. For this reason, we evaluated the performance of graphene-based heaters with from one to five layers of graphene on flexible and transparent polyethylene terephthalate (PET) substrates in terms of their electrothermal properties like heating/cooling rates and steady-state temperatures as a function of the input power density. We found that the heating/cooling rates followed an exponential time dependence with a time constant of just below 6 s for monolayer heaters. From the relationship between the steady-state temperatures and the input power density, a convective heat-transfer coefficient of 60 W·m−2·°C−1 was found, indicating a performance much better than that of many other types of heaters like metal thin-film-based heaters and carbon nanotube-based heaters.
New Benthic Cyanobacteria from Guadeloupe Mangroves as Producers of Antimicrobials
Authors: Duperron Sébastien , Beniddir Mehdi A. , Durand Sylvain , Longeon Arlette , Duval Charlotte , Gros Olivier , Bernard Cécile , Bourguet-Kondracki Marie-Lise
Published: December 2019

Benthic cyanobacteria strains from Guadeloupe have been investigated for the first time by combining phylogenetic, chemical and biological studies in order to better understand the taxonomic and chemical diversity as well as the biological activities of these cyanobacteria through the effect of their specialized metabolites. Therefore, in addition to the construction of the phylogenetic tree, indicating the presence of 12 potentially new species, an LC-MS/MS data analysis workflow was applied to provide an overview on chemical diversity of 20 cyanobacterial extracts, which was linked to antimicrobial activities evaluation against human pathogenic and ichtyopathogenic environmental strains.