bluuebunny/crossref_metadata_2025 huggingface dataset:
Chemsitry Learning Outcomes of 11th Grade Science Students at SMAN 1 Rasau Jaya
Authors: Fitrianti Nenny , Melati Husna Amalya , Rasmawan Rahmat , Sartika Rody Putra , Hadi Lukman
Published: March 2024

This study discusses the ineffective chemistry learning process resulting in poor grades at State Senior High School (SMAN) 1 Rasau Jaya, students tend to receive information from the teacher without constructing their own knowledge, so students have difficulty in constructing the material obtained from the teacher. The purpose of this study is to describe the learning outcomes of students in 11th Grade Science Class odd semester in chemistry subjects at SMAN 1 Rasau Jaya. The form of research used in this research is descriptive research, and the type of research is a case study. Case study is one of qualitative research, in which researchers conduct in-depth exploration of programs, events, processes, activities, for one or more people. The subjects of this study were students of 11th Grade Science Class at SMAN 1 Rasau Jaya in the 2019/2020 school year who took the odd semester general test, totaling 93 students. The research process includes three stages, namely the preparation stage, the implementation stage, and the final stage. The data collection tool used is the odd semester general test score. Results and Discussion regarding Indicator 1: List the general formula of alkane, alkene and alkyne compounds, Indicator 2: Mention the law of conservation of energy, Indicator 3: Determine the number of moles from the results of the experiment, Indicator 4: Determine the value of the equilibrium constant Kc based on the reaction. The results of the general test conducted on 11th Grade students of SMAN 1 Rasau Jaya can be concluded that the learning outcomes of students in the class are not adequate.
An Analysis of Essential Amino Acid Contents of Canistel Fruit (Pouteria campechiana) as A Source of Vegetable Protein in An Effort of Food Diversification
Authors: Harun Andi Ifriany , Sasri Risya , Diannisa Nyemas Firda , Puspasari Yunita
Published: March 2024

Canistel fruit is a fruit that is not cultivated and is not known to many people because of limited information about this fruit. In the field of biochemistry, analysis of the essential amino acids contained in canistel fruit has never been carried out. The aim of this research is to determine the type and levels of essential amino acids in Canistel fruit as a source of vegetable protein in an effort to diversify food. The sample used was Canistel fruit. The method used was chromatography using UPLC with a C18 column, PDA detector, gradient pump system, and column temperature of 49⁰C. 15 types of amino acids were obtained, consisting of 7 essential amino acids and 8 non-essential amino acids. Essential amino acids are L-Fenilalanin (994,64 mg/kg), L- Isoleusin (704,58 mg/kg), L-Valin (876,69 mg/kg), L-Lisin 1.068,36 mg/kg), L- Leusin (1.199,45 mg/kg), L-Treonin (857,16 mg/kg), and LHistidin (421,03 mg/kg)and 8 non-essential amino acid are L-Serin (957,38 mg/kg), L-Asam glutamat (2.137,7 mg/kg), L-Alanin (1.070,01 mg/kg), LArginin (838,15 mg/kg), L-Glisin (1.101,56), L-asam aspartate (1.900,63 mg/kg), L-Tirosin (141,90 mg/kg), and L-Prolin (1.146,84 mg/kg).
Need Analysis for Development (E-Module) of Analytical Chemistry Integrated with Environmental Analysis Research Results
Authors: Yulis Putri Ade Rahma , Oktariani Oktariani
Published: March 2024

The current condition is still limited to analytical chemistry teaching materials that are integrated with research results, only using Analytical Chemistry textbooks as teaching materials or in the form of e-books. This is one of the causes of the lack of student interest in participating in the teaching and learning process. The purpose of this research is to produce innovative teaching materials in analytical chemistry based on research results, aiming to enhance students' interest in learning.This research is the initial stage as a basis for developing analytical chemistry e-modules integrated with research results. The development model used is ADDIE which consists of five stages, namely Analysis, Design, Development, Implementation and Evaluation. This research is limited to the needs analysis stage which includes RPS analysis, analysis of problems and needs of lecturers and problems and needs of students. Data collection instruments used interviews and questionnaires. The subjects of this study consisted of 6 Analytical Chemistry lecturers and 59 students from Riau Province, Riau Islands, North Sumatra, and West Java. The research results of this preliminary analysis include RPS analysis where there are 10 topics of study material and based on the needs questionnaire the topic of quantitative analysis is the topic with the highest percentage to be developed into an e-module. Then also obtained the results that there are still some shortcomings with the teaching materials used today, so that 98% of lecturers and students stated that they need the development of teaching materials, especially those that are digital-based and can be integrated with research results which are evidence of the implementation of analytical chemistry learning theory.
Advancement in Green Synthesis of Titanium Dioxide : Photocatalytic and Larvicidal Activities – A review
Authors: Deliza Deliza , Safni Safni , Zein Rahmiana , Putri Reza Audina
Published: March 2024

Nanotechnology is a fast expanding field with several applications in science, engineering, healthcare, pharmaceutical, and other areas. Nanoparticles (NP) are frequently produced through a variety of physical and chemical methods. Recently, green synthesis technologies that are more simple, sustainable, and cost-effective have been developed. The environmentally friendly/sustainable synthesis of titanium dioxide nanoparticles (TiO2 NPs) has been in great demand in the previous quarter. Bioactive components found in organisms, such as plants, facilitate the processes of bio-reduction and restriction. This review described green synthesis of TiO2 NPs, the photocatalytical uses, and managing the larvicidal activity of disease-spreading mosquitoes. Various natural reducing agents including proteins, enzymes, phytochemicals, and others, are involved in the synthesis of TiO2 NPs. Current research findings and future concerns in a viable platform based on biologically meditated TiO2 nanostructures for industrial applications.
The Effect of Problem Based Learning (PBL) Learning Model on Students' Argumentation Ability on Salt Hydrolysis Material
Authors: Prifes Ramda , Okmarisa Heppy
Published: March 2024

The application of problem-based learning model teaches students how to deal with problems and find solutions by expressing opinions and providing logical justification for real problems, so it is expected to improve students' argumentation skills. This study aims to determine the effect of problem-based learning model on students' argumentation skills on salt hydolysis material. This study was carried out in the even semester of the 2022/2023 academic year at MAN 2 Kampar, class XI IPA.Pretest-posttest Non-Equivalent Control Grub Design was the experimental design for this study, which used quasi-experimental methods. The sample consisted of XI IPA1 students as the experimental class and XI IPA2 as the control class. Data collected to measure students argumentation skills through tests and observations and analyzed using t-test and coefficient of determination test in SPSS 24. In the t-test, the Sig (2-tailed) = 0.000 outcome is more modest than 0.05, and that implies H0 is dismissed and Ha is accepted. For the determination test, the value (r2) = 0.284 is obtained, it can be concluded that the problem-based learning model affects students argumentation skills on salt hydrolysis material by 28.4%.
Chemical Composition Analysis of Citrus×limon (L) Osbeck Leaf Essential Oil and Its Activity as an Anti-Cervical Cancer Agent
Authors: Afif Iffat Syafiqoh , Suryati Suryati , Santoni Adlis
Published: March 2024

Cervical cancer is a life-threatening disease. The use of synthetic drugs for its treatment can have serious adverse effects on the body. Therefore, it is necessary to search for natural drugs that are safe for the body. One of the secondary metabolites contained in plants is essential oils (EO), EO are known to have activity as anti-cervical cancer. Genus Citrus is known for containing EO compounds, one of the species of the genus is Citrus×lemon (L) Osbeck. The purpose of this study is to identify the chemical components of the EO isolated from the leaves of C×limon and to evaluate its cytotoxic activity against cervical cancer. EO was isolated from C×limon leaves collected in Padang City, Indonesia using the hydrodistillation method. EO was then analyzed by GC-MS. To determine its cytotoxicity, BSLT was conducted, followed by molecular docking and MTT assays. The experiment yielded a yellow liquid with a density of 0,8684 g/mL and a yield of 0,181%. GC-MS analysis of the EO identified 56 chemical components, with the main compounds are (-)-β-pinene (7.32%), (-)-limonene (28.40%), geranial (5.54%), caryophyllene (5.22%). The EO showed high toxicity against Artemia salina L larvae, with an LC50 value of 3,697 µg/mL. Through molecular docking, it is known the (-)-limonene, geranial, (-)β-pinene and caryophyllene compounds as the main compounds in of EO are known to bind to form complexes with cervical cancer proteins (HPV18E6). MTT assay showed weak cytotoxic activity of EO against HeLa cells, with an IC50 of 218.9 µg/mL.
PENGARUH PROMOSI MEDIA SOSIAL DAN CITRA MEREK TERHADAP KEPUTUSAN PEMBELIAN KONSUMEN PRODUK SKINCARE SKINTIFIC
Authors: Calistarahma Inas Rafidah , Saputro Edy Purwo
Published: March 2024

Promosi media sosial salah satu media pemasaran digital yang menggunakan platform sosial media melalui link situs web dalam jaringan, untuk tujuan mempromosikan suatu produk. Adanya promosi sosial media sangat mempermudah dalam hal informasi bagi calon konsumen yang ingin membeli produk tertentu. Tujuan dari penelitian ini untuk menganalisis pengaruh promosi dan citra merek terhadap keputusan pembelian konsumen produk skincare skintific. Metode pengumpulan data pada penelitian diatas menggunakan kuesioner. Adapun kajian data menurut BKKBN (Badan Kependudukan dan Keluarga Berencana Nasional) data tersebut disebarkan kepada usia remaja yang berumur 15-35 tahun yang sudah pernah menggunakan skincare skintific. Pada penelitian hasil pembahasan yang dilakukan dalam penelitian ini maka dapat diambil beberapa kesimpulan sebagai berikut: Promosi media sosial berpengaruh positif dan signifikan terhadap keputusan pembelian (Y). dan Citra merk berpengaruh positif terhadap keputusan pembelian produk.
POLA KOMUNIKASI KELOMPOK KOMUNITAS SPARTAN KOMANDO (SPARKO) JAKARTA DALAM MEMPERTAHANKAN EKSISTENSINYA
Authors: Sofiawati Selvi , Pratiwi Wulan Dwi , Pranawukir Iswahyu
Published: March 2024

The Sparko Community is a military-style sports community formed by Lt. Col. Eka Wira in 2015. This community already has many members spread across several cities in Indonesia, one of which is the Sparko GBK Community in Jakarta. The aim of this research is to find out how group communication patterns maintain their existence through the concept of network type theory of communication patterns. The research method is descriptive qualitative. Data collection methods include observation, interviews, literature study and documentation. The informants in this research were represented by 4 people, namely 1 person was a coach as key informant and 3 people were members and community administrators as informants. The research results show that the communication pattern that occurs in the Sparko community is an all-channel communication pattern or a Bintang communication pattern where the chairman and members have the same power in influencing other members and all members can send and receive messages actively and communicatively, and everyone can communicate and participate optimally in the group.

 

Keywords : Communications Pattern, Existence, Sparko Community
PENGARUH AKTIVITAS KOMUNIKASI KRISIS BAKSO AFUNG PADA BRAND TRUST
Authors: Saputro Eko , Sutresna Asep Sutresna
Published: March 2024

Kehalalan produk menjadi hal yang sangat penting di negara seperti Indonesia yang berpenduduk mayoritas orang Islam. Oleh karena itu apabila muncul isu yang berkaitan dengan halal atau tidaknya pada makanan dan minuman yang biasa dikonsumsi orang islam maka dapat memicu krisis. Kasus ini terjadi pada Bakso Afung dimana seorang pelanggan mengonsumsi makanan yang tidak halal yang dibawanya dari tempat lain. Hal ini membuat pemiliknya khawatir akan image produknya yang dapat saja dipersepsikan sebagai makanan atau tempat makan tidak halal. Oleh karena itu pemiliknya melakukan pemecahan mangkok-mangkok untuk menunjukkan adanya pembersihan alat makan yang kemudian diunggah ke media social. Hal ini yang kemudian menarik untuk diteliti, apakah tindakan tersebut dapat mempengaruhi peningkatan brand imagenya. Dengan metode kuantitatif dan dilakukan survey kepada 150 orang responden di Jabodetabek, penelitian ini berusaha untuk mengungkap pengaruh kedua variabelnya. Hasil penelitian menunjukkan adanya pengaruh sebesar 34%. Maka dengan hasil tersebut, perlu dilakukan penelitian lain yang dapat mengungkap faktor-faktor lainnya.

Kata kunci: Bakso Afung, komunikasi krisis, brand image
PENERAPAN SERVICE EXCELLENT OLEH CUSTOMER SERVICE PADA UNIVERSITAS ISLAM SYEKH YUSUF TANGERANG
Authors: Yoman Mad , Pradesti Ajeng
Published: March 2024

This research aims to describe the application of excellent service by Syekh-Yusuf Islamic University to prospective new students. Excellent service focuses on CS abilities, attitudes, appearance, actions, attention and responsibilities in serving customer communications or in this case prospective new students. This research uses a qualitative approach with a case study type. The results of the research show that the excellent assessment at the Syekh-Yusuf Islamic University has been carried out well, but must continue to be improved so that the satisfaction of prospective new students can be achieved well. And training is needed to improve knowledge, skills and appearance in providing excellent service to prospective new students.
STRATEGI KOMUNIKASI PEMASARAN CHEAPRETTY.BIGSIZE DALAM MEMANFAATKAN INSTAGRAM SEBAGAI MEDIA PROMOSI ONLINE
Authors: Umairoh Faikoh
Published: March 2024

Cheapretty.bigsize merupakan online shop yang menjual produk khusus untuk Perempuan yang memiliki badan besar yaitu segmentasi usia 20 s.d 45 tahun yang storenya  berada di laladon, kabupaten bogor. Berdiri sejak tahun 2020 sampai dengan saat ini, cheapretty.bigsize  memikiki pengikut di media sosial instagram  sebanyak 173.000. Dalam mempromosikan produknya secara online secara cheapretty melibatkan instagram. Tujuan penelitian ini untuk mengetahui bagaimana strategi komunikasi pemasaran dari cheapretty big.size dengan memanfaatkan media instagram sebagai promosi online. Metode penelitian ini adalah kualitatif yang bersifat deskriptif. Teknik pengumpulan data yang dilakukan adalah dengan metode observasi, wawancara, studi kepustakaan dan dokumentasi. Key Informan dalam penelitian ini adalah 2 orang dimana keduanya adalah kaka beradik sebagai owner dari cheapretty.bigsize. Teori yang digunakan dalam penelitian ini adalah teori media baru. Hasil dari penelitian ini adalah strategi komunikasi pemasaran yang digunakan cheapretty.bigsize dalam memanfaatkan instagram sebagai media promosi online.Dimana didalam menyusun strategi komunikasi pemasaran harus melalui proses perencanaan diantaranya  segmentasi dan targetting, positioning dan diferensiasi, hubungan Masyarakat dan publikasi, sales promotion, personal selling, direct marketing, internet marketing dan iklan.

 

Kata Kunci : Strategi Komunikasi Pemasaran, Instagram, Cheapretty.bigsize.
Magyarország átfogó egészségvédelmi szűrőprogramjában a vérnyomás és a testsúly adatainak elemzése
Authors: Barna István , Daiki Tenno , Halmy Eszter , Kékes Ede , Dankovics Gergely
Published: March 2024

A Magyarország átfogó egészségvédelmi szűrőprogramja 2010–2020–2030 (MÁESZ) (http://www.egeszsegprogram.eu) Magyarországon az egyetlen komplex szűrővizsgálati program, amelyben hazánk egészségügyi állapotának feltérképezése volt célunk. A 2010–2021 között eltelt időben a kamionban végzett szűrőprogram 2406 helyszínen volt jelen országosan, és több mint 280 000 állampolgár számára több mint kilencmillió szűrővizsgálatot végzett el. A 2010–2021 közötti időszakban nyert adatok alapján elemeztük az egészségi állapotot tükröző paramétereket, illetve az azokat befolyásoló tényezők hatását különböző életkorcsoportokban. A megjelentek testtömegindex-értéke (BMI) átlagosan 53,2%-ban haladta meg a normálértéket, nők esetén ez 46,9%, férfiaknál pedig 60,5% volt. Összességében a nők átlagos vérnyomásértéke 126,7/80,9, a férfiaké 135,6/84,4 Hgmm. Az egyes stádiumok értékelése során több mint 21 000 egyénnél közel 13%-ban mértünk 160/100 Hgmm feletti értéket, közülük több mint 5000 esetben mértünk 180/110 Hgmm feletti vérnyomást. Az elhízás a magas vérnyomásban szenvedők legalább 50%-ánál fordul elő, és gyakoribb a nőknél. A nagyobb hasi zsigeri zsírtömeg nőknél erősen összefügg a magas vérnyomás rizikójával. A testtömegindex a férfiak 60,5%-ában, a nők 47%-ában volt 25 feletti. A szűrőprogram vérnyomásra, túlsúlyra és egyéb, most nem részletezett metabolikus paraméterekre vonatkozó adatai alapján, a statisztika tükrében kijelenthető, hogy a vizsgálatokon részt vevők nagy arányban a nagy kockázatú csoportba tartoznak.
Változások, újdonságok a csecsemőgyógyászati nefrológiában (I. rész) : Vesicoureteral reflux
Authors: Máttyus István
Published: March 2024

Vesicoureteralis reflux (VUR) esetén a vizelet a hólyagból a vesékbe rendellenesen visszaáramlik, ami fokozott vesemedencegyulladás-veszéllyel jár, súlyos esetben vesekárosodást okoz.
Substitusi Tepung Kedelai (Glycine max) dengan Tepung Rumput Laut Caulerpa sp. dalam Pakan Terhadap Performa Pertumbuhan dan Asam Amino Ikan Bandeng (Chanos chanos)
Authors: Liftanto Edhe , Muskita Wellem H. , Kurnia Agus
Published: March 2024

Penelitian ini bertujuan untuk mengetahui pengaruh substitusi tepung kedelai dengan tepung rumput laut Caulerpa sp.dalam pakan pakan terhadap performa pertumbuhan dan asam amino ikan bandeng (Chanos chanos). Empat jenis pakan uji dibuat yang didasarkan pada substitusi tepung kedelai (TK) dan Tepung  Caulerpa (TC) dalam pakan  : 100 % TK + 0 % TC (Pakan A), 75 % TK + 25 % TC (Pakan B), 50 % TK + 50 % TC (Pakan C) dan  25 % TK + 75 % TC (Pakan D). Sebanyak 120 ekor juvenile ikan bandeng (bobot awal rata-rata: 1,06 ± 0,12 g ) dimasukkan ke dalam 12 akuarium (10 ekor/akuarium) berukuran 35 × 34 × 40 cm yang didesain dengan sistem resirkulasi dan diisi dengan air laut yang telah difilter dengan salinitas 32 ± 1,2 ppt. Ikan bandeng diberi pakan uji sebanyak 3 kali sehari (jam 07.00, 12.00 dan 17.00 WITA) sebesar 5 % dari bobot biomassa selama  30 hari pemeliharaan. Penelitian ini didesain dengan menggunakan rancangan acak lengkap dengan mengaplikasikan 4 perlakuan dan 3 ulangan. Parameter yang diamati adalah pertumbuhan mutlak (PM), laju pertumbuhan harian (LPH), kelangsungan hidup (KH), rasio konversi pakan (RKP), efisiensi pakan (EP), konsumsi pakan (KP) dan retensi protein (RP) dan kandungan asam amino tubuh ikan. Hasil penelitian menunjukkan bahwa PM, LPH, dan RKP ikan bandeng masing-masing   berkisar antara 0,15 - 0,23 g, 0,56 – 0,83 % dan 4,34 – 9,87. Nilai EP, KP dan RP ikan bandeng masing-masing berkisar antara, 15,75 – 24,25 %,  15,90 – 16,62 g, dan 0,76 – 1,92%. Tingkat kelangsungan hidup ikan berkisar antara 70 – 83,33 %. Perubahan persentase kandungan asam amino tertinggi dalam tubuh ikan bandeng yaitu jenis asam amino lisin sebesar 97,82%. Penelitian ini menyimpulkan bahwa substitusi 50% tepung kedelai dengan tepung Caulerpa dalam pakan dapat meningkatkan pertumbuhan dan kandungan asam amino tubuh (lisin) ikan bandeng.

Kata kunci : ikan bandeng, pertumbuhan, subtitusi tepung kedelai, tepung Caulerpa sp.
Pengaruh Konsentrasi Tepung Kacang Merah Terhadap Kondisi Morfometrik Usus Ikan Bandeng (Chanos chanos Forskal, 1755)
Authors: Insana Salam Nur , Chadijah Andi , Rahmi
Published: March 2024

Penelitian ini bertujuan untuk mengkaji pengaruh pemberian pakan dengan bahan baku berbagai konsentrasi tepung kacang merah terhadap morfometrik usus ikan bandeng. Kacang merah, dan metode penelitina menggunakan rancangan acak lengkap dengan empat perlakuan dan tiga ulangan masing-masing perlakuan percobaan memeliki konsentrasi tepung kacang merah yang berbeda yaitu Perlakuan A 0% tepung kacang merah. Perlakuan B 20% tepung kacang merah. Perlakuan C 30%,  tepung kacang merah, dan Perlakuan D 40% tepung kacang merah. Parameter pengamatan panjang usus relative, dan panjang rata-rata vili usus. Hasil penelitian menunjukkan bahwa panajang usus relative ikan bandeng anatara perlakuan tidak berbeda nyata anatar perlakuan (P>0.05). Secara kuantitatif, panjang usus relative tertinggi terdapat pada perlakuan C yaitu sebasar 216%, sedangkan panjang terendah terdapat pada perlakuan B yaitu 201%, Panjang rata-rata vili usus, tertinggi terdapat pada perlakuan D yaitu 40.42 μm, sedangkan terendah perlakuan A 25.21 μm dan Penambahan tepung kacang merah dengan konsentrasi 30% dalam pakan menunjukkan morfometrik usus yang baik terhadap penjang usus relatif dan panjang rata-rata vili usus.

Kata Kunci: Bandeng,  Konsentrasi, Kacang Merah, Morfometrik, Usus
Sebaran Pelagis Besar Hasil Tangkapan Purse Seine di Indonesia
Authors: Mardiah Ratu Sari , Krisnafi Yaser , Pramesthy Tyas Dita
Published: March 2024

Purse seine catches that have high economic value are big pelagic whose distribution of fishing areas and landing sites are poorly known. This study aims to find out the distribution of large pelagic purse seine catches based on WPP and their landing ports. The data used is data of purse seine catch from the Ministry of Maritime Affairs and Fisheries of the Republic of Indonesia in 2019 and 2020 to be analyzed descriptively. The results showed that four big pelagic species caught by purse seines: tuna (Thunnus sp.), mackerel (Scomberomorus commerson), marlin (Istiophorus platypterus) and swordfish (Xiphias gladius). Tuna is caught in all WPP and landed at 62 fishing ports. Mackerel was also caught in all WPP and landed at 74 fishing ports. Marlin were caught at 10 WPP and landed at 27 fishing ports. Swordfish were caught in 4 WPP and landed at 5 fishing ports. Knowledge on distribution of big pelagic purse seine catches based on WPP and landing ports can be used as a basis for evaluating measured fishery policies in terms of catch landing relating to fishing areas.
Karakteristik Hasil Tangkapan Jaring Insang di Desa Teteaji, Kabupaten Sidenreng Rappang
Authors: Oktaviany Revy , Hasrianti  , Bibin Muhammad
Published: March 2024

Danau Sidenreng tepatnya di Kabupaten Sidenreng Rappang yang berfungsi sebagai penghasil ikan yang dimanfaatkan oleh masyarakat dan meningkatkan pendapatan para nelayan. Penelitian dilaksanakan pada bulan januari - maret 2023 dan penelitian berlokasi di Desa Teteaji, Kabupaten Sidenreng Rappang, Provinsi Sulawesi Selatan. Pada alat tangkap jaring insang yang dioperasikan nelayan di Danau Sidenreng menggunakan perahu mesin tempel, ukuran mesin kapal menggunakan 7 sampai 13 PK. Panjang kapal 4 sampai 7 meter dan lebar 89 cm. Alat tangkap jaring insang yang digunakan di Danau Sidenreng mempunyai panjang 80 meter/set, rata-rata yang dioperasikan nelayan memiliki panjang 400 meter sampai 3200 meter. Ikan hasil tangkapan jaring insang dari seluruh sampel data yang paling dominan tertangkap pada jaring adalah ikan tawes (barbonymus gonionotus) adalah 1.284 ekor atau 60%. Ukuran mata jaring yang digunakan nelayan yaitu 4 cm, 4,5 cm, 5 cm, 5,5 cm, 6 cm dan 8 cm. Ukuran ikan yang tertangkap pada jaring insang sangat dipengaruhi oleh ukuran mata jaring (mesh size) semakin besar ukuran mata jaring semakin besar pula ukuran hasil tangkapan. Jaring dengan mesh size 4 banyak menangkap ikan yang belum matang gonad dan belum layak tangkap yang ikut tertangkap pada jaring. Ukuran mata jaring yang menangkap ikan yang layak tangkap (matang gonad) TKG 4 adalah jaring dengan mesh size  5 cm.
Profil Asam Amino dan Pertumbuhan Ikan Kerapu Cantang (Epinephelus lanceolatus × Epinephelus fuscoguttatus) yang Diberi Jenis Tepung Ikan Berbeda
Authors: Muh. Nuryadin  , Kurnia Agus , Muskita Wellem H. , Hamzah Muhaimin , Idris Muhammad , Yusnaini
Published: March 2024

Penelitian ini bertujuan untuk mengetahui profil asam amino dan pertumbuhan Ikan Kerapu Cantang (Epinephelus lanceolatus × Epinephelus fuscoguttatus) yang diberi jenis tepung ikan yang berbeda. Tiga jenis pakan uji yang mengandung tepung ikan layang dan ikan tembang dibuat  pada penelitian ini  adalah  100 % tepung ikan tembang (Pakan A), 50% Tepung ikan tembang +50% Tepung ikan layang (Pakan B) dan 100% tepung ikan layang (Pakan C). Sebanyak 120 ekor ikan kerapu cantang (bobot awal rata-rata: 6.77± 0.64 g) dimasukkan ke dalam 12 akuarium (10 ekor per akuarium) berukuran 60×50 ×40 cm. Pemberian pakan ikan uji dilakukan sebanyak 2 kali dalam sehari secara ad libitum atau pemberian sampai kenyang selama 45 hari. Penelitian dirancang menggunakan rancangan acak lengkap (RAL), terdiri dari tiga perlakuan dan empat ulangan. Variabel yang diamati meliputi pertumbuhan mutlak, kelangsungan hidup, dan kandungan asam amino tubuh ikan. Hasil penelitian menunjukkan bahwa pemberian pakan uji tidak memberikan pengaruh nyata terhadap pertumbuhan mutlak ikan kerapu cantang.  Jenis asam amino tubuh ikan kerapu yang diberi pakan uji yang berbeda menunjukkan asam amino tertinggi adalah leusin (10865 mg/kg tubuh) dan terendah adalah histidin (3904 mg/kg tubuh). Profil asam amino tubuh ikan kerapu cantang pada awal pemeliharaan adalah sama dengan profil asam amino tubuh ikan uji pada akhir penelitian.  Penelitian ini menyimpulkan bahwa jenis dan jumlah asam amino yang ada dalam pakan ikan tembang, tepung ikan layang dan campuran keduanya sesuai dengan kebutuhan asam amino tubuh ikan kerapu cantang.
Distribusi dan Kelimpahan Family Atherinidae di Perairan Desa Tanjung Tiram, Kecamatan Moramo Utara, Kabupaten Konawe Selatan
Authors: Asriyana  , Halili  , Trisma
Published: March 2024

Penelitian ini bertujuan untuk mengetahui pola penyebaran dan kelimpahan ikan famili Atherinidae serta mengetahui parameter kualitas air yang memengaruhi keberadaan famili tersebut di perairan Tanjung Tiram. Sampel ikan ditangkap menggunakan jaring insang berukuran panjang 12 m dan lebar 105 cm dengan ukuran mata jaring 3/4 inci. Selama penelitian tertangkap 811 individu yang termasuk dalam tiga spesies yaitu A. lacunocus, A. duodecimalis  dan H. temminckii. Ikan yang tertangkap memiliki kisaran panjang 70-110 sampai 75-105 mm dan bobot 2,85-15,4 sampai 3.17-12.44 g. Ketiga spesies tersebut mempunyai pola distribusi mengelompok (2> 1). A. lacunocus ditemukan dengan kelimpahan tertinggi di semua stasiun berturut-turut di Stasiun I, II, dan III, yaitu 22,125 ind/trip; 39,5 ind/trip; dan 30,125 ind/trip. Kondisi kualitas air di perairan Desa Tanjung Tiram dalam kondisi yang layak untuk kehidupan organisme perairan. Sebanyak 284 individu didominasi oleh juvenil (ukuran 7,0 cm) dan 512 individu didominasi oleh ikan dewasa (8,0-9,0 cm) yang cenderung mengasosiasikan diri dengan ekosistem mangrove dan padang lamun. Hasil ini menunjukkan bahwa perairan Tanjung Tiram cocok untuk ikan famili Atherinidae sebagai tempat mencari makan dan daerah asuhan.
Pengaruh Salinitas yang Berbeda Terhadap Kepadatan Thalassiosira sp. Skala Laboratorium
Authors: Dara Azzahra , Surianti  , Hasrianti
Published: March 2024

Thalassiosira sp. adalah salah satu jenis plankton mikroalga yang biasanya digunakan sebagai pakan alami bagi larva udang Salinitas adalah salah satu faktor yang sangat penting dalam budidaya diatom laut, karena dapat berpengaruh terhadap pertumbuhan diatom. Penelitian bertujuan untuk mengetahui pengaruh salinitas yang berbeda terhadap dinamika populasi dan kualitas air Thalassiosira sp. dan menentukan salinitas yang terbaik terhadap dinamika populasi dan kualitas air Thalassiosira sp. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 pengulangan. Adapun perlakuan A yaitu salinitas 20 ppt, perlakuan B yaitu salinitas 25 ppt, perlakuan C yaitu salinitas 30 ppt dan perlakuan D yaitu salinitas 35 ppt. Hasil penelitian menunjukkan bahwa perlakuan dengan menggunakan salinitas yang berbeda memberikan pengaruh nyata terhadap dinamika populasi dan kualitas air Thalassiosira sp. Salinitas terbaik untuk tingkat kepadatan Thalassiosira sp. terdapat pada perlakuan C dengan salinitas 30 ppt yaitu 47.5 x 104 sel/ml yang tertinggi dan perlakuan dengan salinitas terendah berada pada perlakuan D dengan salinitas 35 ppt yaitu 37.4 x 104 sel/ml. Hasil pengukuran kualitas air dengan perlakuan yang menggunakan salinitas yang berbeda (20 ppt, 25 ppt, 30 ppt, dan 35 ppt) menunjukkan nilai kualitas air (suhu, pH, dan intensitas cahaya) masih dalam batas toleransi atau kelanyakan hidup (Baik) Thalassiosira sp.
Detecting subtle transcriptomic perturbations induced by lncRNAs knock-down in single-cell CRISPRi screening using a new sparse supervised autoencoder neural network
Authors: Truchi Marin , Lacoux Caroline , Gille Cyprien , Fassy Julien , Magnone Virginie , Lopes Goncalves Rafael , Girard-Riboulleau Cédric , Manosalva-Pena Iris , Gautier-Isola Marine , Lebrigand Kevin , Barbry Pascal , Spicuglia Salvatore , Vassaux Georges , Rezzonico Roger , Barlaud Michel , Mari Bernard
Published: March 2024

Single-cell CRISPR-based transcriptome screens are potent genetic tools for concomitantly assessing the expression profiles of cells targeted by a set of guides RNA (gRNA), and inferring target gene functions from the observed perturbations. However, due to various limitations, this approach lacks sensitivity in detecting weak perturbations and is essentially reliable when studying master regulators such as transcription factors. To overcome the challenge of detecting subtle gRNA induced transcriptomic perturbations and classifying the most responsive cells, we developed a new supervised autoencoder neural network method. Our Sparse supervised autoencoder (SSAE) neural network provides selection of both relevant features (genes) and actual perturbed cells. We applied this method on an in-house single-cell CRISPR-interference-based (CRISPRi) transcriptome screening (CROP-Seq) focusing on a subset of long non-coding RNAs (lncRNAs) regulated by hypoxia, a condition that promote tumor aggressiveness and drug resistance, in the context of lung adenocarcinoma (LUAD). The CROP-seq library of validated gRNA against a subset of lncRNAs and, as positive controls, HIF1A and HIF2A, the 2 main transcription factors of the hypoxic response, was transduced in A549 LUAD cells cultured in normoxia or exposed to hypoxic conditions during 3, 6 or 24 h. We first validated the SSAE approach on HIF1A and HIF2 by confirming the specific effect of their knock-down during the temporal switch of the hypoxic response. Next, the SSAE method was able to detect stable short hypoxia-dependent transcriptomic signatures induced by the knock-down of some lncRNAs candidates, outperforming previously published machine learning approaches. This proof of concept demonstrates the relevance of the SSAE approach for deciphering weak perturbations in single-cell transcriptomic data readout as part of CRISPR-based screening.
MYC: there is more to it than cancer
Authors: Zacarías-Fluck Mariano F. , Soucek Laura , Whitfield Jonathan R.
Published: March 2024

MYC is a pleiotropic transcription factor involved in multiple cellular processes. While its mechanism of action and targets are not completely elucidated, it has a fundamental role in cellular proliferation, differentiation, metabolism, ribogenesis, and bone and vascular development. Over 4 decades of research and some 10,000 publications linking it to tumorigenesis (by searching PubMed for “MYC oncogene”) have led to MYC becoming a most-wanted target for the treatment of cancer, where many of MYC’s physiological functions become co-opted for tumour initiation and maintenance. In this context, an abundance of reviews describes strategies for potentially targeting MYC in the oncology field. However, its multiple roles in different aspects of cellular biology suggest that it may also play a role in many additional diseases, and other publications are indeed linking MYC to pathologies beyond cancer. Here, we review these physiological functions and the current literature linking MYC to non-oncological diseases. The intense efforts towards developing MYC inhibitors as a cancer therapy will potentially have huge implications for the treatment of other diseases. In addition, with a complementary approach, we discuss some diseases and conditions where MYC appears to play a protective role and hence its increased expression or activation could be therapeutic.
Anti-leishmanial activity of Eleutherine plicata Herb. and predictions of isoeleutherin and its analogues
Authors: Albuquerque Kelly Cristina Oliveira de , Veiga Andreza do Socorro Silva da , Silveira Fernando Tobias , Campos Marliane Batista , Costa Ana Paula Lima da , Brito Ananda Karolyne Martins , Melo Paulo Ricardo de Souza , Percario Sandro , Molfetta Fábio Alberto de , Dolabela Maria Fâni
Published: March 2024

Introduction: Leishmaniasis is caused by protozoa of the genus Leishmania, classified as tegumentary and visceral. The disease treatment is still a serious problem, due to the toxic effects of available drugs, the costly treatment and reports of parasitic resistance, making the search for therapeutic alternatives urgent. This study assessed the in vitro anti-leishmanial potential of the extract, fractions, and isoeleutherin from Eleutherine plicata, as well as the in silico interactions of isoeleutherin and its analogs with Trypanothione Reductase (TR), in addition to predicting pharmacokinetic parameters.

Methods: From the ethanolic extract of E. plicata (EEEp) the dichloromethane fraction (FDEp) was obtained, and isoeleutherin isolated. All samples were tested against promastigotes, and parasite viability was evaluated. Isoeleutherin analogues were selected based on similarity in databases (ZINK and eMolecules) to verify the impact on structural change.

Results and Discussion: The extract and its fractions were not active against the promastigote form (IC50 > 200 μg/mL), while isoeleutherin was active (IC50 = 25 μg/mL). All analogues have high intestinal absorption (HIA), cell permeability was moderate in Caco2 and low to moderate in MDCK. Structural changes interfered with plasma protein binding and blood-brain barrier permeability. Regarding metabolism, all molecules appear to be CYP3A4 metabolized and inhibited 2–3 CYPs. Molecular docking and molecular dynamics assessed the interactions between the most stable configurations of isoeleutherin, analogue compound 17, and quinacrine (control drug). Molecular dynamics simulations demonstrated stability and favorable interactions with TR. In summary, fractionation contributed to antileishmanial activity and isoleutherin seems to be promising. Structural alterations did not contribute to improve pharmacokinetic aspects and analogue 17 proved to be more promising than isoeleutherin, presenting better stabilization in TR.
Case Report: Role of numerical simulations in the management of acute aortic syndromes
Authors: Rizza Antonio , Castiglione Vincenzo , Capellini Katia , Palmieri Cataldo , Gasparotti Emanuele , Berti Sergio , Celi Simona
Published: March 2024

Penetrating aortic ulcer (PAU) represents a subset of acute aortic syndromes characterized by high rupture risk and management challenges, particularly in elderly patients with significant comorbidities. This case report showcases a 75-year-old patient with a history of coronary artery bypass graft (CABG) and with multiple PAUs involving the aortic arch, deemed unfit for conventional open surgery. A branched aortic endograft with a pre-cannulated side component for the left subclavian artery (LSA) was employed to preserve the patency of the previous CABG. Two computational fluid dynamics (CFD) simulations and a morphological analysis were performed on the pre- and post-intervention aortic configurations to evaluate changes in flow rate and pressure drop at LSA level and differences in the lumen size. The results revealed a decrease in the flow rate equal to 2.38% after the intervention and an increase in pressure drop of 4.48 mmHg, while the maximum differences in LSA cross-sectional areas and diameters were 1.49 cm2 and 0.64 cm, respectively. Minimal alteration in LSA blood flow due to the chosen intervention approach confirmed the effectiveness of the selected unibody design endograft with LSA preservation, ensuring myocardial perfusion. Therefore, CFD simulations demonstrate to be a powerful tool to evaluate the hemodynamic consequences of interventions by accurately estimating the main fluid dynamic parameters.
Prenatal transposition of great arteries diagnosis and management: a Chinese single-center study
Authors: Guo Jie , Ling Wen , Dang Tingting , Guo Shan , Ma Hong , Huang Qiong , Zeng Liqin , Weng Zongjie , Wu Qiumei
Published: March 2024

Objective

This study aimed to assess the diagnostic value of prenatal echocardiography for identifying transposition of the great arteries (TGA) during pregnancy and evaluating the associated outcomes.

Methods

We conducted a retrospective analysis of 121 prenatally diagnosed patients with TGA at our hospital between January 2012 and September 2022. This analysis included prenatal ultrasound, prenatal screening, clinical management and follow-up procedures.

Results

Among the 103 fetuses considered in the study, 90 (87.4%) were diagnosed with complete transposition of the great arteries (D-TGA), while 13 (12.6%) exhibited corrected transposition of the great arteries (CC-TGA). Diagnoses were distributed across the trimester, with 8 D-TGA and 2 CC-TGA patients identified in the first trimester, 68 D-TGA patients and 9 CC-TGA patients in the second trimester, and 14 D-TGA and 2 CC-TGA patients referred for diagnosis in the third trimester. Induction of labour was pursued for 76 D-TGA patients (84.4%) and 11 CC-TGA patients (84.6%), and 14 D-TGA patients (15.6%) and 2 CC-TGA patients (15.4%) continued pregnancy until delivery. Among the D-TGA patients, 9 fetuses (10.0%) underwent surgery, two of which were inadvertent fatality, while the remaining seven experienced positive outcomes. Additionally, seven TGA patients received palliative care, leading to four fatalities among D-TGA patients (5.2%), whereas 1 D-TGA patients and 2 CC-TGA patients survived.

Conclusion

This study underscores the feasibility of achieving an accurate prenatal diagnosis of TGA during early pregnancy. The utility of prenatal ultrasound in the development of personalized perinatal plans and the application of multidisciplinary treatment during delivery are conducive.
Increased levels of a mycophenolic acid metabolite in patients with kidney failure negatively affect cardiomyocyte health
Authors: Harlacher Eva , Schulte Corinna , Vondenhoff Sonja , Schmitt-Kopplin Philippe , Diederich Philippe , Hemmers Christian , Moellmann Julia , Wollenhaupt Julia , Veltrop Rogier , Biessen Erik , Lehrke Michael , Peters Björn , Schlieper Georg , Kuppe Christoph , Floege Jürgen , Jankowski Vera , Marx Nikolaus , Jankowski Joachim , Noels Heidi
Published: March 2024

Chronic kidney disease (CKD) significantly increases cardiovascular risk and mortality, and the accumulation of uremic toxins in the circulation upon kidney failure contributes to this increased risk. We thus performed a screening for potential novel mediators of reduced cardiovascular health starting from dialysate obtained after hemodialysis of patients with CKD. The dialysate was gradually fractionated to increased purity using orthogonal chromatography steps, with each fraction screened for a potential negative impact on the metabolic activity of cardiomyocytes using a high-throughput MTT-assay, until ultimately a highly purified fraction with strong effects on cardiomyocyte health was retained. Mass spectrometry and nuclear magnetic resonance identified the metabolite mycophenolic acid-β-glucuronide (MPA-G) as a responsible substance. MPA-G is the main metabolite from the immunosuppressive agent MPA that is supplied in the form of mycophenolate mofetil (MMF) to patients in preparation for and after transplantation or for treatment of autoimmune and non-transplant kidney diseases. The adverse effect of MPA-G on cardiomyocytes was confirmed in vitro, reducing the overall metabolic activity and cellular respiration while increasing mitochondrial reactive oxygen species production in cardiomyocytes at concentrations detected in MMF-treated patients with failing kidney function. This study draws attention to the potential adverse effects of long-term high MMF dosing, specifically in patients with severely reduced kidney function already displaying a highly increased cardiovascular risk.
Multidimensional digital biomarker phenotypes for mild cognitive impairment: considerations for early identification, diagnosis and monitoring
Authors: Milner Tracy , Brown Matthew R. G. , Jones Chelsea , Leung Ada W. S. , Brémault-Phillips Suzette
Published: March 2024

Mild Cognitive Impairment (MCI) poses a challenge for a growing population worldwide. Early identification of risk for and diagnosis of MCI is critical to providing the right interventions at the right time. The paucity of reliable, valid, and scalable methods for predicting, diagnosing, and monitoring MCI with traditional biomarkers is noteworthy. Digital biomarkers hold new promise in understanding MCI. Identifying digital biomarkers specifically for MCI, however, is complex. The biomarker profile for MCI is expected to be multidimensional with multiple phenotypes based on different etiologies. Advanced methodological approaches, such as high-dimensional statistics and deep machine learning, will be needed to build these multidimensional digital biomarker profiles for MCI. Comparing patients to these MCI phenotypes in clinical practice can assist clinicians in better determining etiologies, some of which may be reversible, and developing more precise care plans. Key considerations in developing reliable multidimensional digital biomarker profiles specific to an MCI population are also explored.
Multi-omics subgroups associated with glycaemic deterioration in type 2 diabetes: an IMI-RHAPSODY Study
Authors: Li Shiying , Dragan Iulian , Tran Van Du T. , Fung Chun Ho , Kuznetsov Dmitry , Hansen Michael K. , Beulens Joline W. J. , Hart Leen M. ‘t , Slieker Roderick C. , Donnelly Louise A. , Gerl Mathias J. , Klose Christian , Mehl Florence , Simons Kai , Elders Petra J. M. , Pearson Ewan R. , Rutter Guy A. , Ibberson Mark
Published: March 2024

Introduction

Type 2 diabetes (T2D) onset, progression and outcomes differ substantially between individuals. Multi-omics analyses may allow a deeper understanding of these differences and ultimately facilitate personalised treatments. Here, in an unsupervised “bottom-up” approach, we attempt to group T2D patients based solely on -omics data generated from plasma.

Methods

Circulating plasma lipidomic and proteomic data from two independent clinical cohorts, Hoorn Diabetes Care System (DCS) and Genetics of Diabetes Audit and Research in Tayside Scotland (GoDARTS), were analysed using Similarity Network Fusion. The resulting patient network was analysed with Logistic and Cox regression modelling to explore relationships between plasma -omic profiles and clinical characteristics.

Results

From a total of 1,134 subjects in the two cohorts, levels of 180 circulating plasma lipids and 1195 proteins were used to separate patients into two subgroups. These differed in terms of glycaemic deterioration (Hazard Ratio=0.56;0.73), insulin sensitivity and secretion (C-peptide, p=3.7e-11;2.5e-06, DCS and GoDARTS, respectively; Homeostatic model assessment 2 (HOMA2)-B; -IR; -S, p=0.0008;4.2e-11;1.1e-09, only in DCS). The main molecular signatures separating the two groups included triacylglycerols, sphingomyelin, testican-1 and interleukin 18 receptor.

Conclusions

Using an unsupervised network-based fusion method on plasma lipidomics and proteomics data from two independent cohorts, we were able to identify two subgroups of T2D patients differing in terms of disease severity. The molecular signatures identified within these subgroups provide insights into disease mechanisms and possibly new prognostic markers for T2D.
Multi-scenario simulation of land use dynamics and ecological risk: a case study of the liaohe estuary national wetland reserve using PLUS-Markov and PSR models
Authors: Hu Nanlin , Wang Guodong , Ma Zijun , Zhao Meiling , Yuan Yusong , Zhang Tao , Chen Qi , Meng Jingci , Wang Jiaying
Published: March 2024

Introduction: Wetland has been exposed to tremendous stresses and hazards, leading to many potential ecological risks in the past few decades. There is an urgent need to assess the ecological risk status of the wetland, especially when examining how the intensity of socio-economic growth, policy changes, and other variables affect land use and ecological risk changes.

Methods: This study models the LULC pattern in the Liaohe Estuary National Nature Wetland Reserve under various future scenarios in 2000–2040 and develops a long-term Pressure-State-Response ecological risk assessment model based on the characteristics of the northern wetland environment in China, combined with the simulated multi-scenario PLUS model.

Results: As the two most distinct vegetation types in the reserve area, Phragmites australis and Suaeda glauca presented decreasing trends of 59.7 ha/year and 9.0 ha/year in the economy development scenario (EDS), higher than 57.3 ha/year, 8.2 ha/year in the natural increase scenario (NIS), and 35.4 ha/year and 5.8 ha/year in the ecological protect scenario (EPS). From the core area to the buffer area to the outer experimental area, the slope rate of vegetation deterioration rises severely. In comparison to the scenario of EDS, the area of aquiculture and oil wells can be lowered by 11.4 ha/year and 1.1 ha/year with the application of ecological protection measures. Besides, under three scenarios, mean ecological risks are all showing an increasing trend from 2000 to 2040, which is generally higher in the EDS scenario than that in the NIS and EPS scenarios. The proportion of the high and very high level of ecological risk area continually increased from 28.8% to 40.4% from 2000 to 2040, which was mainly located in the south estuary and west urban areas. In addition, among the three protected areas, the ecological risk in the core area has a lower growth rate than that in the outer buffer zone and experimental zone.

Discussion: Aiming for the development of Liaohe Estuary National Park, these findings provide quantitative guidance for protecting and restoring natural resources.