bluuebunny/crossref_metadata_2025 huggingface dataset:
Hybrid System Assessment in On-Grid and Off-Grid Conditions: A Technical and Economical Approach
Authors: Tabora Jonathan Muñoz , Paixão Júnior Ulisses Carvalho , Rodrigues Carlos Eduardo Moreira , Bezerra Ubiratan Holanda , Tostes Maria Emília de Lima , Albuquerque Bruno S. de , Matos Edson Ortiz de , Nascimento Andréia Antloga do
Published: August 2021

Photovoltaic systems can strengthen the energy matrix aiming at energy sustainability, however, their intermittence, the availability time frame and seasonality effects are complicating aspects for the operation of these systems. To mitigate such problems, a possible measure is the operation of hybrid systems (HS) associated with storage systems to meet the energy demand. This study presents real operational scenarios for the management of a hybrid mini-grid installed in the Amazon region in Brazil, capable of managing energy supply and demand, changing the generation curve, reducing fossil fuel consumption and energy costs, in addition to providing flexibility and operational safety to the electrical operating under on-grid or off-grid conditions. Through technical and economic analyses, the objective of this study is to show that hybrid systems can be implemented in small and medium consumers, in addition to generating a new market option for utilities. This research evaluates the operation of HS concurrently with the electrical grid and the results show that the system meets the technical requirements, in addition to bringing financial and ecological benefits. This information can support the decisions of consumers, utilities and energy operators for the development of the national market for on-grid and off-grid HS.
Effects of Water Injection Strategies on Oxy-Fuel Combustion Characteristics of a Dual-Injection Spark Ignition Engine
Authors: Li Xiang , Pei Yiqiang , Li Dayou , Ajmal Tahmina , Rana Khaqan-Jim , Aitouche Abdel , Mobasheri Raouf , Peng Zhijun
Published: August 2021

Currently, global warming has been a serious issue, which is closely related to anthropogenic emission of Greenhouse Gas (GHG) in the atmosphere, particularly Carbon Dioxide (CO2). To help achieve carbon neutrality by decreasing CO2 emissions, Oxy-Fuel Combustion (OFC) technology is becoming a hot topic in recent years. However, few findings have been reported about the implementation of OFC in dual-injection Spark Ignition (SI) engines. This work numerically explores the effects of Water Injection (WI) strategies on OFC characteristics in a practical dual-injection engine, including GDI (only using GDI), P50-G50 (50% PFI and 50% GDI) and PFI (only using PFI). The findings will help build a conceptual and theoretical foundation for the implementation of OFC technology in dual-injection SI engines, as well as exploring a solution to increase engine efficiency. The results show that compared to Conventional Air Combustion (CAC), there is a significant increase in BSFC under OFC. Ignition delay (θF) is significantly prolonged, and the spark timing is obviously advanced. Combustion duration (θC) of PFI is a bit shorter than that of GDI and P50-G50. There is a small benefit to BSFC under a low water-fuel mass ratio (Rwf). However, with the further increase of Rwf from 0.2 to 0.9, there is an increment of 4.29%, 3.6% and 3.77% in BSFC for GDI, P50-G50 and PFI, respectively. As WI timing (tWI) postpones to around −30 °CA under the conditions of Rwf ≥ 0.8, BSFC has a sharp decrease of more than 6 g/kWh, and this decline is more evident under GDI injection strategy. The variation of maximum cylinder pressure (Pmax) and combustion phasing is less affected by WI temperature (TWI) compared to the effects of Rwf or tWI. BSFC just has a small decline with the increase of TWI from 298 K to 368 K regardless of the injection strategy. Consequently, appropriate WI strategies are beneficial to OFC combustion in a dual-injection SI engine, but the benefit in fuel economy is limited.
Feature Extraction, Ageing Modelling and Information Analysis of a Large-Scale Battery Ageing Experiment
Authors: de Oliveira Jose Genario , Dhingra Vipul , Hametner Christoph
Published: August 2021

Large scale testing of newly developed Li-ion cells is associated with high costs for the interested parties, and ideally, testing time should be kept to a minimum. In this work, an ageing model was developed and trained with real data from a large-scale testing experiment in order to answer how much testing time and data would have been really needed to achieve similar model generalisation performance on previously unseen data. A linear regression model was used, and the feature engineering, extraction and selection steps are shown herein, alongside accurate prediction results for the majority of the accelerated ageing experiments. Information analysis was performed to achieve the desired data reduction, obtaining similar model properties with a fifth of the number of cells and half of the testing time. The proposed ageing model uses features commonly found in the literature, and the structure is simple enough for the training to be performed online in an EV. It has good generalisation capabilities. Lastly, the data reduction approach used here is model-independent, allowing a similar methodology to be used with different modelling assumptions.
Energy Management Strategy for an Electromechanical-Hydraulic Coupled Power Electric Vehicle Considering the Optimal Speed Threshold
Authors: Meng Zewen , Zhang Tiezhu , Zhang Hongxin , Zhao Qinghai , Yang Jian
Published: August 2021

Considering the problems of the low energy recovery efficiency and the short driving range of pure electric vehicles, a new electromechanical–hydraulic coupled power electric vehicle is proposed. First, we develop an electromechanical–hydraulic coupled power electric vehicle model and design an energy management strategy to match it. On this basis, an optimization strategy is proposed with the goal of improving the braking energy recovery efficiency and avoiding the impact of high-speed braking energy recovery on the hydraulic system. The energy recovery mode conversion is optimized for different vehicle speeds when braking. Finally, the proposed optimization strategy is verified by joint simulation. The results show that when the vehicle speed is higher than 10 m/s for energy recovery mode switching, the total recovery efficiency of the whole vehicle increases to 97.273% and the SOC of the power battery increases by 0.14%. This provides strong support for improving the driving range of electromechanical–hydraulic coupled power electric vehicles.
Diagnostic Protocol for Thermal Performance of District Heating Pipes in Operation. Part 2: Estimation of Present Thermal Conductivity in Aged Pipe Insulation
Authors: Lidén Peter , Adl-Zarrabi Bijan , Hagentoft Carl-Eric
Published: August 2021

Buried and operating district heating (DH) pipes are exposed to thermal degradation of their polyurethane (PUR) insulation over time, and their status is hard to assess without excavation. By using DH pipe valves in manholes as measurement points during a shutdown with an ensuing cooling period, non-destructive assessments can be performed. This study compares new improved field measurements with numerical simulations of the temperature decline in drainage valves and shutdown valves. The drainage valve measurements were used to thermally assess part of a buried DH network. Results indicate that by using the drainage valves as measurement points in a cooling method, the thermal conductivity of the buried DH network could be predicted with an accuracy of >95%. In addition, a general diagnostic protocol has been established for assessing the thermal status of a DH network, ready for network owners to use.
Sensory Characterization of Iberian Dry-Cured Loins by Using Check-All-That-Apply (CATA) Analysis and Multiple-Intake Temporal Dominance of Sensations (TDS)
Authors: González-Mohino Alberto , Ventanas Sonia , Estévez Mario , Olegario Lary Souza
Published: August 2021

The aim of the present work was to sensorially characterize different commercial categories of Iberian dry-cured loins (varying genetic and feeding background) using a novel dynamic sensory technique, and to explore consumers preferences applying a rapid method. The samples (green label—GL, Cebo de Campo Ibérico; red label—RL, Bellota 50% Ibérico; and black label—BL, Bellota 100%) were analyzed by (i) Check-all-that-apply (CATA) with the evaluation of an ‘Ideal’ dry-cured loin and the overall liking, and by (ii) multiple-intake Temporal Dominance of Sensations (TDS). The CATA results indicated that the sensory characteristics of RL samples were closer to those of the ‘Ideal’ loin. Furthermore, juiciness, marbling, cured flavor, chewiness, persistence, and brightness were selected as ‘must-have’ attributes. Juiciness cured flavor and red color were considered as drivers of liking. TDS results showed that flavor attributes presented the highest dominance rates, with saltiness being the most dominant attribute along BL sample evaluation, and with cured and paprika flavor for GL and RL samples. These sensory technique results displayed the ability to sensorily characterize dry-cured loins, providing different, complementary, and valuable information.
Ishige okamurae Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells
Authors: Kim Mingyeong , Cho Chiheung , Lee Changjun , Ryu Bomi , Kim Sera , Hur Jinyoung , Lee Sang-Hoon
Published: August 2021

Advanced glycation end-products (AGEs) such as methylglyoxal (MGO) play a vital role in the pathogenesis of nephropathy, a diabetic complication. In the present study, we evaluated the anti-glycation and renal protective properties of Ishige okamurae extract (IOE) against AGE-induced oxidative stress. HPLC analysis confirmed that bioactive phlorotannins such as diphlorethohydroxycarmalol and ishophloroglucin A are predominantly present in IOE. IOE showed strong anti-glycation activities via inhibition of AGE formation, inhibition of AGE–protein cross-linking, and breaking of AGE–protein cross-links. In addition, in vitro studies using mesangial cells demonstrated that IOE effectively suppressed intracellular reactive oxygen species production, intracellular MGO accumulation, and apoptotic cell death by MGO-induced oxidative stress, in addition to regulating the expression of proteins involved in the receptor for AGEs and nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response elements (ARE) signaling pathways. Therefore, IOE can serve as a natural therapeutic agent for the management of AGE-related nephropathy.
“More and More Fond of Reading”: Everything You Wanted to Know about Transgender Studies but Were Afraid to Ask Clara Reeve
Authors: Huthwaite Desmond
Published: August 2021

Clara Reeve’s (1729–1807) Gothic novel The Old English Baron is a node for contemplating two discursive exclusions. The novel, due to its own ambiguous status as a gendered “body”, has proven a difficult text for discourse on the Female Gothic to recognise. Subjected to a temperamental dialectic of reclamation and disavowal, The Old English Baron can be made to speak to the (often) subordinate position of Transgender Studies within the field of Queer Studies, another relationship predicated on the partial exclusion of undesirable elements. I treat the unlikely transness of Reeve’s body of text as an invitation to attempt a trans reading of the bodies within the text. Parallel to this, I develop an attachment genealogy of Queer and Transgender Studies that reconsiders essentialism―the kind both practiced by Female Gothic studies and also central to the logic of Reeve’s plot―as a fantasy that helps us distinguish where a trans reading can depart from a queer one, suggesting that the latter is methodologically limited by its own bad feelings towards the former.
Personalized Diet in Obesity: A Quasi-Experimental Study on Fat Mass and Fat-Free Mass Changes
Authors: García-Galbis Manuel Reig , Gallardo Diego I. , Martínez-Espinosa Rosa María , Soto-Méndez María José
Published: August 2021

Considering that the prevalence of overweight and obesity in Southeast of Spain is high, the aim of this work was to analyze the relation between the adherence to a personalized diet and the effectiveness of changes in the body composition in overweight and obese adults in this region. This quasi-experimental study presents the following selection criteria: attendance at the consultation between 2006 and 2012, subjects ≥ 19 years of age with overweight or obesity. In total, 591 overweight or obese individuals were involved in this study, attending 4091 clinic consultations in total. Most of the sample consisted of subjects who attended >3 consultations (>1.5 months), and were females aged 19–64 years who obtained clinically significant changes in fat mass (FM, ≥5%) and recommended changes in fat-free mass (FFM, ≥0%). Based on the results obtained and the experience gained from this research, the following recommendations are established: (i) record fat mass and fat-free mass index as a complement to body mass index; (ii) use FM and FFM to evaluate effectiveness of interventions with the aim of obtaining body composition changes; (iii) use personalized diet to achieve significant changes in FM and avoid non-recommended changes in FFM.
A Review of Monitoring Methods for Cerebral Blood Oxygen Saturation
Authors: Zhong Wentao , Ji Zhong , Sun Changlong
Published: August 2021

In recent years, cerebral blood oxygen saturation has become a key indicator during the perioperative period. Cerebral blood oxygen saturation monitoring is conducive to the early diagnosis and treatment of cerebral ischemia and hypoxia. The present study discusses the three most extensively used clinical methods for cerebral blood oxygen saturation monitoring from different aspects: working principles, relevant parameters, current situations of research, commonly used equipment, and relative advantages of different methods. Furthermore, through comprehensive comparisons of the methods, we find that near-infrared spectroscopy (NIRS) technology has significant potentials and broad applications prospects in terms of cerebral oxygen saturation monitoring. Despite the current NIRS technology, the only bedside non-invasive cerebral oxygen saturation monitoring technology, still has many defects, it is more in line with the future development trend in the field of medical and health, and will become the main method gradually.
Can Ketogenic Diet Therapy Improve Migraine Frequency, Severity and Duration?
Authors: Haslam Rebecca L. , Bezzina Aaron , Herbert Jaimee , Spratt Neil , Rollo Megan E. , Collins Clare E.
Published: August 2021

Migraine is the third most common condition worldwide and is responsible for a major clinical and economic burden. The current pilot trial investigated whether ketogenic diet therapy (KDT) is superior to an evidence-informed healthy “anti-headache” dietary pattern (AHD) in improving migraine frequency, severity and duration. A 12-week randomised controlled crossover trial consisting of the two dietary intervention periods was undertaken. Eligible participants were those with a history of migraines and who had regularly experienced episodes of moderate or mildly intense headache in the previous 4 weeks. Migraine frequency, duration and severity were assessed via self-report in the Migraine Buddy© app. Participants were asked to measure urinary ketones and side effects throughout the KDT. Twenty-six participants were enrolled, and 16 participants completed all sessions. Eleven participants completed a symptom checklist; all reported side-effects during KDT, with the most frequently reported side effect being fatigue (n = 11). All completers experienced migraine during AHD, with 14/16 experiencing migraine during KDT. Differences in migraine frequency, severity or duration between dietary intervention groups were not statistically significant. However, a clinically important trend toward lower migraine duration on KDT was noted. Further research in this area is warranted, with strategies to lower participant burden and promote adherence and retention.
Influence of Phosphite Supply in the MS Medium on Root Morphological Characteristics, Fresh Biomass and Enzymatic Behavior in Five Genotypes of Potato (Solanum tuberosum L.)
Authors: Dormatey Richard , Sun Chao , Ali Kazim , Qin Tianyuan , Xu Derong , Bi Zhenzhen , Bai Jiangping
Published: August 2021

Crop production is threatened by low phosphorus (P) availability and weed interference. Obtaining plant genotypes that can utilize Phosphite (Phi) as fertilizer can supplement phosphates (Pi) while providing an environmentally friendly means of weed control. The study was conducted to determine the tolerance and enzymatic behavior of five potato genotypes to PO3. Explants were regenerated in vitro from two nodal cuttings and cultured on Murashige and Skoog (MS) medium under controlled conditions for 30 days. Matured plantlets were subcultured for 20 days in MS medium containing (0.25, 0.5 mM) Phi and Pi and No-P (-Phi + -Pi). The results showed significant genotypic variation in tolerance indices among the five genotypes. Atlantic showed greater tolerance to Phi, with highest total root length (50.84%), root projected area (75.09%), root surface area (68.94%), root volume (33.49%) and number of root forks (75.66%). Phi induced an increasing trend in the levels of hydrogen peroxide in the genotypes with the least effect in Atlantic. The comprehensive evaluation analysis confirmed the tolerance of Atlantic genotype with this ranking; Atlantic, Longshu3, Qingshu9, Longshu6 and Gannong2. Antioxidant enzyme activities and proline content also increased significantly under Phi and No-P treatments. The results suggested that potato genotypes with larger root systems may be more tolerant to Phi than genotypes with smaller root systems.
What Are the Restraint Practices, Preferences, and Experiences When Australian Parents Travel with Their Children in a Rideshare Vehicle?
Authors: Koppel Sjaan , Peiris Sujanie , Aburumman Mohammed , Wong Chernyse W. R. , Owens Justin M. , Womack Katie N.
Published: August 2021

This study aimed to explore the preferences, experiences and restraint practices of Australian parents travelling with their children in rideshare vehicles. Six hundred and thirty-one participants completed an online survey (M = 39.2 years, SD = 10.5, Range = 18.0–70.0 years; Female: 63.4%). Most participants (59.1%) reported that they had not travelled in a rideshare vehicle with their youngest child (M = 7.2 years, SD = 5.2, Range = 0.0–17.0 years; Male: 54.2%). Participants who reported that they have travelled with their youngest child in a rideshare vehicle tended to: be younger, identify as male, have completed an Undergraduate or Postgraduate degree, reside in the Australian Capital Territory, earning a higher yearly household income, and were involved in an at-fault crash in the past two years. In addition, these participants were: less likely to have a ‘younger’ youngest child, less likely to ‘always’ wear a seatbelt while travelling in their private motor vehicle, and also less likely to ‘always’ restrain their child in an appropriate restraint while travelling in their private motor vehicle. Prohibitive reasons for not travelling in a rideshare vehicle included: cost (29.3%), concerns over driver safety (27.5%), concerns over travelling with children in a rideshare service (24.8%), or inconvenience (24.3%). Participants who reported that they had travelled in a rideshare vehicle with their youngest child reported lower rates of appropriate restraint use within the rideshare vehicle (57.3%) than when travelling in their private motor vehicle (85.6%). Reasons associated with inappropriate restraint use within the rideshare vehicle included: unavailability of a child restraint (39.6%), travelling a short distance (33.0%), were not required to use one in this situation (33.0%), or the parent did not have a restraint with them (26.4%). Given the increasing popularity of rideshare services in Australia, and globally, the urgent adaption of rideshare-specific policy, legislation, education, and design in relation to child restraint requirements is needed to ensure the safety of child occupants.
“I Just Want Some Clear Answers”: Challenges and Tactics Adopted by Migrants in Denmark When Accessing Health Risk Information about COVID-19
Authors: Brønholt Rasmus Luca Lyager , Langer Primdahl Nina , Jensen Anja M. B. , Verelst An , Derluyn Ilse , Skovdal Morten
Published: August 2021

Health risk communication plays a crucial role in preventing the spread of infectious disease outbreaks such as the current coronavirus (SARS-CoV-2). Yet, migrants are far too often forgotten in health risk communication responses. We investigate the challenges and efforts made by migrants in Denmark—in the initial months of the pandemic—to access information about COVID-19. We draw on 18 semi-structured interviews conducted in May and June 2020. All interviews are thematically coded and analyzed. Our analysis reveals that many of the migrants faced several challenges, including accessing information in a language understandable to them and navigating constant streams of official news flows issuing instructions about which actions to take. However, we also note that the participating migrants found numerous creative ways to address some of these challenges, often aided by digital tools, helping them access crucial health and risk information. This paper highlights that migrants constitute an underserved group in times of crises. They are vulnerable to getting left behind in pandemic communication responses. However, we also identify key protective factors, social resources, and agentic capabilities, which help them cope with health and risk information deficits. National governments need to take heed of these findings to inform future pandemic responses.
Effect of Personal and Contextual Factors of Regulation on Academic Achievement during Adolescence: The Role of Gender and Age
Authors: de la Fuente Jesús , Malpica-Chavarria Erika Andrea , Garzón-Umerenkova Angélica , Pachón-Basallo Mónica
Published: August 2021

This investigation aimed to analyze the predictive differential value of personal (self-regulation, self-efficacy, procrastination) and contextual characteristics (parents’ socio-educational level), regarding academic achievement, among Colombian adolescents. A total of 430 students (from 11 to 18 years old) from both genders filled out validated self-reports and informed their academic achievement. We performed an ex-post-facto design, simple regression analyses, structural equations predictions analyses (SEM), and variance analyses (ANOVAs). The results showed that self-regulation is the most potent personal variable predictive of procrastination and achievement, positively associated with self-efficacy; additionally, the parents’ educational level was also a predictor, although to a lesser level. The female group and the elderly group negatively predicted academic achievement, behaving as modulatory variables of the above results.
The Impact of the Integrated Development of AI and Energy Industry on Regional Energy Industry: A Case of China
Authors: Dong Feng , Zhang Shengnan , Zhu Jiao , Sun Jiaojiao
Published: August 2021

With the advent of the Energy 4.0 era, the adoption of “Internet + artificial intelligence” systems will enable the transformation and upgrading of the traditional energy industry. This will alleviate the energy and environmental problems that China is currently facing. The integrated development of artificial intelligence and the energy industry has become inevitable in the development of future energy systems. This study applied a comprehensive evaluation index to the energy industry to calculate the comprehensive development index of the energy industry in 30 provinces of China from 2000 to 2017. Then, taking Guangdong and Jiangsu as examples, the synthetic control method was used to explore the direction and intensity of the integrated development of artificial intelligence and the energy industry on the comprehensive development level of the local energy industry. The results showed that when artificial intelligence (AI) and the energy industry achieved a stable coupled development without the need to move to the coordination stage, the coupling effect promoted the development of the regional energy industry, and the annual growth rate of the comprehensive development index was above 20%. This coupling effect passed the placebo test and ranking test and was significant at the 10% level, indicating the robustness and validity of the experimental results, which strongly confirmed the great potential of AI in re-empowering traditional industries from the data perspective. Based on the findings, corresponding policy recommendations were proposed on how to promote the development of inter-regional AI, how the government, enterprises, and universities could cooperate to promote the coordinated development of AI and energy, and how to guide the integration process of regional AI and energy industries according to local conditions, in order to maximize the technological dividend of AI and help the construction of smart energy in China.
Is the Clinical Delivery of Cardiac Rehabilitation in an Australian Setting Associated with Changes in Physical Capacity and Cardiovascular Risk and Are Any Changes Maintained for 12 Months?
Authors: Price Kym Joanne , Gordon Brett Ashley , Bird Stephen Richard , Benson Amanda Clare
Published: August 2021

Long-term maintenance of changes in cardiovascular risk factors and physical capacity once patients leave the supervised program environment have not previously been reported. This study investigated the changes in physical capacity outcomes and cardiovascular risk factors in an Australian cardiac rehabilitation setting, and the maintenance of changes in these outcomes in the 12 months following cardiac rehabilitation attendance. Improvements in mean (95% CI) cardiorespiratory fitness (16.4% (13.2–19.6%), p < 0.001) and handgrip strength (8.0% (5.4–10.6%), p < 0.001) were observed over the course of the cardiac rehabilitation program, and these improvements were maintained in the 12 months following completion. Waist circumference (p = 0.003) and high-density lipoprotein cholesterol (p < 0.001) were the only traditional cardiovascular risk factors to improve during the cardiac rehabilitation program. Vigorous-intensity aerobic exercise was associated with significantly greater improvements in cardiorespiratory fitness, Framingham risk score, and waist circumference in comparison to moderate-intensity exercise. An increase in the intensity of the exercise prescribed during cardiac rehabilitation in Australia is recommended to induce larger improvements in physical capacity outcomes and cardiovascular risk. A standardized exercise test at the beginning of the rehabilitation program is recommended to facilitate appropriate prescription of exercise intensity.
In Situ Measurement of Airborne Particle Concentration in a Real Dental Office: Implications for Disease Transmission
Authors: Razavi Maryam , Butt Zahid Ahmad , Chen Helen , Tan Zhongchao
Published: August 2021

Aerosols generated during dental procedures are one of the most significant routes for infection transmission and are particularly relevant now in the context of COVID-19 pandemic. This study aimed to assess the effectiveness of an indoor air purifier on dental aerosol dispersion in dental offices. The spread and removal of aerosol particles generated from a specific dental operation in a dental office are quantified for a single dental activity in the area near the generation and corner of the office. The effects of the air purifier, door condition, and particle sizes on the spread and removal of particles were investigated. The results show that, in the worst-case scenario, it takes 95 min for 0.5-μm particles to settle and that it takes a shorter time for the larger particles. The air purifier expedited the removal time at least 6.3 times faster than the case with no air purifier in the generation zone. Our results also indicate that particles may be transported from the source to the rest of the room even when the particle concentrations in the generation zone dropped back to the background. Therefore, it is inaccurate to conclude that indoor purifiers help reduce the transmission of COVID-19. Dental offices still need other methods to reduce the transmission of viruses.
Oro-Dental Manifestations in a Pediatric Patient Affected by Helsmoortel-Van der Aa Syndrome
Authors: Petruzzi Massimo , Stella Alessandro , Capra Valeria , Contaldo Maria , della Vella Fedora
Published: August 2021

Aim: Aim of this case report is to describe oro-facial abnormalities in a patient affected by Helsmoortel-Van der Aa syndrome, a rare autism syndrome, with not well described dental and cranial malformations. Case Report: Helsmoortel-Van der Aa Syndrome is a rare autosomal genetic syndrome causing mental impairment and autism, craniofacial dysmorphism, chest deformity and multiple organs dysfunction. Oro-facial involvement in Helsmoortel-Van der Aa syndrome has not been thoroughly described yet. The present article reports a case of a 9 years old male patient affected by Helsmoortel-Van der Aa Syndrome, presenting with oral breathing typical facies, high arched palate, II class and dental crowding. The patient teething was adequate to his age. The enamel of incisors and molars showed demineralization areas and dark spots, a clinical picture consistent with molar incisor hypomineralization syndrome. These hypo-mineralized areas are more susceptible to cavities, in fact the patient’s 4.6 tooth was decayed. The child was brought to our attention due to a mucocele on the lower lip, confirmed by histopathologic examination. Available data on oro-dental manifestation of this syndrome are rather poor and inconsistent, also due to the rarity of the disease. The finding of enamel abnormalities in the presented case could suggest a potential genetic etiopathogenesis linked to the same genes causing Helsmoortel-Van der Aa syndrome.
Is It Possible to Have Home E-Monitoring of Pulmonary Function in Our Patients with Duchenne Muscular Dystrophy in the COVID-19 Pandemic?—A One Center Pilot Study
Authors: Wasilewska Eliza , Sobierajska-Rek Agnieszka , Małgorzewicz Sylwia , Soliński Mateusz , Szalewska Dominika , Jassem Ewa
Published: August 2021

Background: Duchenne muscular dystrophy (DMD) is the most common, progressive, irreversible muscular dystrophy. Pulmonary function is crucial for duration of life in this disease. Currently, the European Respiratory Society is focused on digital health, seeking innovations that will be realistic for digital respiratory medicine to support professionals and patients during the COVID-19 pandemic. Aims: The aim of this study was to investigate whether it is possible to monitor pulmonary function at home using an individual electronic spirometry system in boys with Duchenne muscular dystrophy. Materials and methods: In this observational, prospective study, conducted from March 2021 to June 2021, twenty boys with DMD (aged 8–16) were enrolled. The patients were recruited from the Rare Disease Centre, University Clinical Centre, of Gdańsk, Poland. Medical history and anthropometric data were collected, and spirometry (Jaeger, Germany) was performed in all patients at the start of the study. Each patient received an electronic individual spirometer (AioCare) and was asked to perform spirometry on their own every day, morning and evening, at home for a period of 4 weeks. The number of measurements, correctness of performing measurements, forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), and peak expiratory flow (PEF) were evaluated. Results: Finally, 14 out of 20 boys enrolled in the study with a mean age of 12.5 years (7 non-ambulatory) applied and received a home spirometer (AioCare). A total of 283 measurements were performed by all patients at home for 4 weeks. Half of the patients were able to perform measurements correctly. There were no significant differences between mean values of FVC, FE1, PEF between home and hospital spirometry (p > 0.05) expect PEF pv% (p < 0.00046). Patients with higher FEV1 (p = 0.0387) and lower BMI (p = 0.0494) were more likely to take home spirometer measurements. The mean general satisfaction rating of home-spirometry was 4.33/5 (SD 0.78), the mean intelligibility rating was 4.83/5 (SD 0.58). Reasons for irregular measurements were: forgetting (43%), lack of motivation (29%), difficulty (14%), lack of time (14%). Conclusion: Home electronic monitoring of pulmonary function in patients with DMD is possible to implement in daily routines at home. This protocol should be introduced as early as possible in patients 7–8 years old with good, preserved lung function. Patients accept this form of medical care but require more education about the benefits of e-monitoring. There is a need to implement a system to remind patients of the use of electronic medical devices at home, e.g., via SMS (short message service).
Sex Differences in the Morphological and Mechanical Properties of the Achilles Tendon
Authors: Zhang Xini , Deng Liqin , Xiao Songlin , Li Lu , Fu Weijie
Published: August 2021

Background: Patients with Achilles tendon (AT) injuries are often engaged in sedentary work because of decreasing tendon vascularisation. Furthermore, men are more likely to be exposed to AT tendinosis or ruptures. These conditions are related to the morphological and mechanical properties of AT, but the mechanism remains unclear. This study aimed to investigate the effects of sex on the morphological and mechanical properties of the AT in inactive individuals. Methods: In total, 30 inactive healthy participants (15 male participants and 15 female participants) were recruited. The AT morphological properties (cross-sectional area, thickness, and length) were captured by using an ultrasound device. The AT force–elongation characteristics were determined during isometric plantarflexion with the ultrasonic videos. The AT stiffness was determined at 50%–100% maximum voluntary contraction force. The AT strain, stress, and hysteresis were calculated. Results: Male participants had 15% longer AT length, 31% larger AT cross-sectional area and 21% thicker AT than female participants (p < 0.05). The plantarflexion torque, peak AT force, peak AT stress, and AT stiffness were significantly greater in male participants than in female participants (p < 0.05). However, no significant sex-specific differences were observed in peak AT strain and hysteresis (p > 0.05). Conclusions: In physically inactive adults, the morphological properties of AT were superior in men but were exposed to higher stress conditions. Moreover, no significant sex-specific differences were observed in peak AT strain and hysteresis, indicating that the AT of males did not store and return elastic energy more efficiently than that of females. Thus, the mechanical properties of the AT should be maintained and/or improved through physical exercise.
Open Issues in the Transition from NAFLD to MAFLD: The Experience of the Plinio Study
Authors: Baratta Francesco , Ferro Domenico , Pastori Daniele , Colantoni Alessandra , Cocomello Nicholas , Coronati Mattia , Angelico Francesco , Del Ben Maria
Published: August 2021

Metabolic associated fatty liver diseases (MAFLD) definition was proposed to identify fatty liver condition associated to metabolic disorders and to replace non-alcoholic fatty liver disease (NAFLD). We aimed to explore the effect of the application of the new MAFLD criteria on a pre-existing cohort of NAFLD patients. The consequences of the reclassification were investigated by applying the MAFLD criteria to a prospective cohort (The Plinio Study) of dysmetabolic patients examined for the presence of NAFLD. In the Plinio cohort, 795 patients had NAFLD and 767 of them (96.5%) were reclassified as MAFLD patients. Out of these, 94.9% had overweight/obesity or diabetes, while the remaining were lean and had metabolic dysregulation defined by the presence of at least two metabolic risk abnormalities. By contrast, 3.5% of the NAFLD patients were reclassified as no-MAFLD due to the absence of overweight/obesity, diabetes, or metabolic risk abnormalities. The only significant difference between the NAFLD and MAFLD groups was the higher prevalence of subjects with BMI ≥ 25 kg/m2 in the latter (88.6% vs. 92%; p = 0.018). In the cohort, 68 subjects were defined as “lean NAFLD”. Of these, 40 were reclassified as MAFLD and 28 as no-MAFLD. In conclusion, when applying MAFLD criteria to the Plinio cohort, there is a substantial overlap between NAFLD and MAFLD diagnosis. However, some specific subgroups of patients, such as those currently defined as lean NAFLD, were excluded by the new MAFLD definition.
Risk Prediction of Second Primary Endometrial Cancer in Obese Women: A Hospital-Based Cancer Registry Study
Authors: Chang Chi-Chang , Chen Chun-Chia , Cheewakriangkrai Chalong , Chen Ying Chen , Yang Shun-Fa
Published: August 2021

Due to the high effectiveness of cancer screening and therapies, the diagnosis of second primary cancers (SPCs) has increased in women with endometrial cancer (EC). However, previous studies providing adequate evidence to support screening for SPCs in endometrial cancer are lacking. This study aimed to develop effective risk prediction models of second primary endometrial cancer (SPEC) in women with obesity (body mass index (BMI) > 25) and included datasets on the incidence of SPEC and the other risks of SPEC in 4480 primary cancer survivors from a hospital-based cancer registry database. We found that obesity plays a key role in SPEC. We used 10 independent variables as predicting variables, which correlated to obesity, and so should be monitored for the early detection of SPEC in endometrial cancer. Our proposed scheme is promising for SPEC prediction and demonstrates the important influence of obesity and clinical data representation in all cases following primary treatments. Our results suggest that obesity is still a crucial risk factor for SPEC in endometrial cancer.
How Much Does HIV Positivity Affect the Presence of Oral HPV? A Molecular Epidemiology Survey
Authors: Visalli Giuseppa , Di Pietro Angela , Currò Monica , Pruiti Ciarello Marianna , D’Andrea Flavia , Nunnari Giuseppe , Pellicanò Giovanni Francesco , Facciolà Alessio
Published: August 2021

HIV-positive people showed a high oral prevalence of HPV-DNA and have a greater incidence of head and neck carcinomas compared to general population. We performed a molecular survey evaluating the presence of HPV-DNA in saliva of HIV-positive and HIV-negative subjects in order to quantify the risk represented by HIV-positivity. The sample was made up by 102 subjects: 40 HIV-positive, 32 HIV-negative with sexual risk behaviors (SRB) and 30 HIV-negative without risk factors. DNA was extracted from cellular pellets and HPV detection and genotyping were performed by PCR assays. In the HIV-positive group (of which 58.3% declared SRB) 33.33% of the sample were HPV-positive (33.33% to high-risk genotypes, 25.0% to low-risk genotypes and 41.66% to other genotypes). In the HIV-negative SRB group, HPV-positive subjects were 37.04% (60.0% to high risk genotypes, 20.0% to low risk genotypes, and 20.0% to other genotypes). Finally, in the control group, the HPV-positive subjects were 7.14% (50% to high-risk genotypes and 50% to low-risk genotypes). In the HIV group, concerning the HPV positivity, there was no significant difference between subjects with and without SRBs. In summary, we found a high oral HPV-DNA detection in HIV+ group, showing a strong relationship between HIV and HPV.
Gap Junction Channelopathies and Calmodulinopathies. Do Disease-Causing Calmodulin Mutants Affect Direct Cell–Cell Communication?
Authors: Peracchia Camillo
Published: August 2021

The cloning of connexins cDNA opened the way to the field of gap junction channelopathies. Thus far, at least 35 genetic diseases, resulting from mutations of 11 different connexin genes, are known to cause numerous structural and functional defects in the central and peripheral nervous system as well as in the heart, skin, eyes, teeth, ears, bone, hair, nails and lymphatic system. While all of these diseases are due to connexin mutations, minimal attention has been paid to the potential diseases of cell–cell communication caused by mutations of Cx-associated molecules. An important Cx accessory protein is calmodulin (CaM), which is the major regulator of gap junction channel gating and a molecule relevant to gap junction formation. Recently, diseases caused by CaM mutations (calmodulinopathies) have been identified, but thus far calmodulinopathy studies have not considered the potential effect of CaM mutations on gap junction function. The major goal of this review is to raise awareness on the likely role of CaM mutations in defects of gap junction mediated cell communication. Our studies have demonstrated that certain CaM mutants affect gap junction channel gating or expression, so it would not be surprising to learn that CaM mutations known to cause diseases also affect cell communication mediated by gap junction channels.
Chemical Chaperones Modulate the Formation of Metabolite Assemblies
Authors: Adsi Hanaa , Levkovich Shon A. , Haimov Elvira , Kreiser Topaz , Meli Massimiliano , Engel Hamutal , Simhaev Luba , Karidi-Heller Shai , Colombo Giorgio , Gazit Ehud , Laor Bar-Yosef Dana
Published: August 2021

The formation of amyloid-like structures by metabolites is associated with several inborn errors of metabolism (IEMs). These structures display most of the biological, chemical and physical properties of protein amyloids. However, the molecular interactions underlying the assembly remain elusive, and so far, no modulating therapeutic agents are available for clinical use. Chemical chaperones are known to inhibit protein and peptide amyloid formation and stabilize misfolded enzymes. Here, we provide an in-depth characterization of the inhibitory effect of osmolytes and hydrophobic chemical chaperones on metabolite assemblies, thus extending their functional repertoire. We applied a combined in vivo-in vitro-in silico approach and show their ability to inhibit metabolite amyloid-induced toxicity and reduce cellular amyloid content in yeast. We further used various biophysical techniques demonstrating direct inhibition of adenine self-assembly and alteration of fibril morphology by chemical chaperones. Using a scaffold-based approach, we analyzed the physiochemical properties of various dimethyl sulfoxide derivatives and their role in inhibiting metabolite self-assembly. Lastly, we employed whole-atom molecular dynamics simulations to elucidate the role of hydrogen bonds in osmolyte inhibition. Our results imply a dual mode of action of chemical chaperones as IEMs therapeutics, that could be implemented in the rational design of novel lead-like molecules.
Arboviruses: How Saliva Impacts the Journey from Vector to Host
Authors: Schneider Christine A. , Calvo Eric , Peterson Karin E.
Published: August 2021

Arthropod-borne viruses, referred to collectively as arboviruses, infect millions of people worldwide each year and have the potential to cause severe disease. They are predominately transmitted to humans through blood-feeding behavior of three main groups of biting arthropods: ticks, mosquitoes, and sandflies. The pathogens harbored by these blood-feeding arthropods (BFA) are transferred to animal hosts through deposition of virus-rich saliva into the skin. Sometimes these infections become systemic and can lead to neuro-invasion and life-threatening viral encephalitis. Factors intrinsic to the arboviral vectors can greatly influence the pathogenicity and virulence of infections, with mounting evidence that BFA saliva and salivary proteins can shift the trajectory of viral infection in the host. This review provides an overview of arbovirus infection and ways in which vectors influence viral pathogenesis. In particular, we focus on how saliva and salivary gland extracts from the three dominant arbovirus vectors impact the trajectory of the cellular immune response to arbovirus infection in the skin.
Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance
Authors: Ayaz Asma , Huang Haodong , Zheng Minglü , Zaman Wajid , Li Donghai , Saqib Saddam , Zhao Huayan , Lü Shiyou
Published: August 2021

Cutin and wax are the main precursors of the cuticle that covers the aerial parts of plants and provide protection against biotic and abiotic stresses. Long-chain acyl-CoA synthetases (LACSs) play diversified roles in the synthesis of cutin, wax, and triacylglycerol (TAG). Most of the information concerned with LACS functions is obtained from model plants, whereas the roles of LACS genes in Glycine max are less known. Here, we have identified 19 LACS genes in Glycine max, an important crop plant, and further focused our attention on 4 LACS2 genes (named as GmLACS2-1, 2, 3, 4, respectively). These GmLACS2 genes display different expression patterns in various organs and also show different responses to abiotic stresses, implying that these genes might play diversified functions during plant growth and against stresses. To further identify the role of GmLACS2-3, greatly induced by abiotic stresses, we transformed a construct containing its full length of coding sequence into Arabidopsis. The expression of GmLACS2-3 in an Arabidopsis atlacs2 mutant greatly suppressed its phenotype, suggesting it plays conserved roles with that of AtLACS2. The overexpression of GmLACS2-3 in wild-type plants significantly increased the amounts of cutin and suberin but had little effect on wax amounts, indicating the specific role of GmLACS2-3 in the synthesis of cutin and suberin. In addition, these GmLACS2-3 overexpressing plants showed enhanced drought tolerance. Taken together, our study deepens our understanding of the functions of LACS genes in different plants and also provides a clue for cultivating crops with strong drought resistance.
Extracellular Vesicles as Emerging Players in Intercellular Communication: Relevance in Mast Cell-Mediated Pathophysiology
Authors: Shefler Irit , Salamon Pazit , Mekori Yoseph A.
Published: August 2021

Mast cells are major effector cells in eliciting allergic responses. They also play a significant role in establishing innate and adaptive immune responses, as well as in modulating tumor growth. Mast cells can be activated upon engagement of the high-affinity receptor FcεRI with specific IgE to multivalent antigens or in response to several FcεRI-independent mechanisms. Upon stimulation, mast cells secrete various preformed and newly synthesized mediators. Emerging evidence indicates their ability to be a rich source of secreted extracellular vesicles (EVs), including exosomes and microvesicles, which convey biological functions. Mast cell-derived EVs can interact with and affect other cells located nearby or at distant sites and modulate inflammation, allergic response, and tumor progression. Mast cells are also affected by EVs derived from other cells in the immune system or in the tumor microenvironment, which may activate mast cells to release different mediators. In this review, we summarize the latest data regarding the ability of mast cells to release or respond to EVs and their role in allergic responses, inflammation, and tumor progression. Understanding the release, composition, and uptake of EVs by cells located near to or at sites distant from mast cells in a variety of clinical conditions, such as allergic inflammation, mastocytosis, and lung cancer will contribute to developing novel therapeutic approaches.