bluuebunny/crossref_metadata_2025 huggingface dataset:
Separation of Five Flavonoids from Aerial Parts of Salvia Miltiorrhiza Bunge Using HSCCC and Their Antioxidant Activities
Authors: Yang Fan , Qi Yingxue , Liu Wei , Li Jia , Wang Daijie , Fang Lei , Zhang Yongqing
Published: September 2019

The aerial parts of Salvia miltiorrhiza Bunge, as the non-medicinal parts, are always discarded during harvesting, resulting in a huge waste of resources and environmental pressure. Due to the high flavonoid content and their antioxidant activities characteristics, the aerial parts of S. miltiorrhiza can be developed into natural antioxidants and used in foods. A high-speed counter-current chromatography (HSCCC) method, using a two-phase solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water (3:1:1:20, v/v), was the first to successfully isolate five flavonoids from the aerial parts of S. miltiorrhiza in one attempt, and separately categorized as rutin (1), isoquercitrin (2), kaempferol-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (3), kaempferol-3-O-β-d-glucopyranoside (4) and apigenin-7-O-β-d-glucopyranoside (5) after identification. The purities of these plant isolates were 97.3%, 99.5%, 92.8%, 98.1% and 98.7%, respectively. All the flavonoids were identified by HR-ESI-MS, 1D and 2D NMR. Compounds 3 and 5 were firstly isolated from the plant of S. miltiorrhiza. Results from antioxidant assays showed that rutin (1) and isoquercitrin (2) had higher antioxidant capacities compared to L-ascorbic acid as the positive control.
Synthesis, Physicochemical Properties, and Biological Activities of 4-(S-Methyl-N-(2,2,2-Trifluoroacetyl)Sulfilimidoyl) Anthranilic Diamide
Authors: Lim Hwan Jung , Lee Won Hyung , Park Seong Jun
Published: September 2019

Novel anthranilic diamides with sulfilimidoyl and sulfoximidoyl functionalities were successfully prepared. Among newly-prepared organosulfur compounds, 3-bromo-1-(3-chloropyridin-2-yl)-N-(2-methyl-6-(methylcarbamoyl)-4-(methylthio)phenyl)-1H-pyrazole-5-carboxamide and (S,E)-3-bromo-1-(3-chloropyridin-2-yl)-N-(2-methyl-4-(S-methyl-N-(2,2,2-trifluoroacetyl)sulfinimidoyl)-6-(methylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide showed good levels of efficacy and a strong correlation between insecticidal activities and physical properties, respectively. In particular, available data indicated that the N-trifluoroacetyl sulfilimine moiety could be an appealing structural scaffold for the discovery of a new crop-protecting agent.
Towards Designing Diegetic Gaze in Games: The Use of Gaze Roles and Metaphors
Authors: Ramirez Gomez Argenis , Lankes Michael
Published: September 2019

Gaze-based interactions have found their way into the games domain and are frequently employed as a means to support players in their activities. Instead of implementing gaze as an additional game feature via a game-centred approach, we propose a diegetic perspective by introducing gaze interaction roles and gaze metaphors. Gaze interaction roles represent ambiguous mechanics in gaze, whereas gaze metaphors serve as narrative figures that symbolise, illustrate, and are applied to the interaction dynamics. Within this work, the current literature in the field is analysed to seek examples that design around gaze mechanics and follow a diegetic approach that takes roles and metaphors into account. A list of surveyed gaze metaphors related to each gaze role is presented and described in detail. Furthermore, a case study shows the potentials of the proposed approach. Our work aims at contributing to existing frameworks, such as EyePlay, by reflecting on the ambiguous meaning of gaze in games. Through this integrative approach, players are anticipated to develop a deeper connection to the game narrative via gaze, resulting in a stronger experience concerning presence (i.e., being in the game world).
A Simple and Efficient Method for Designing Broadband Terahertz Absorber Based on Singular Graphene Metasurface
Authors: Liu Zhongmin , Guo Liang , Zhang Qingmao
Published: September 2019

In this paper, we propose a simple and efficient method for designing a broadband terahertz (THz) absorber based on singular graphene patches metasurface and metal-backed dielectric layer. An accurate circuit model of graphene patches is used for obtaining analytical expressions for the input impedance of the proposed absorber. The input impedance is designed to be closely matched to the free space in a wide frequency range. Numerical simulation and analytical circuit model results consistently show that graphene metasurface-based THz absorber with an absorption value above 90% in a relative bandwidth of 100% has been achieved.
Review on Quasi One-Dimensional CdSe Nanomaterials: Synthesis and Application in Photodetectors
Authors: Jin Weifeng , Hu Luodan
Published: September 2019

During the past 15 years, quasi one-dimensional (1D) Cadmium Selenide (CdSe) nanomaterials have been widely investigated for high-performance electronic and optoelectronic devices, due to the unique geometrical and physical properties. In this review, recent advancements on diverse synthesis methods of 1D CdSe nanomaterials and the application in photodetectors have been illustrated in detail. First, several bottom-up synthesis methods of 1D CdSe nanomaterials have been introduced, including the vapor-liquid-solid method, the solution-liquid-solid method, and electrochemical deposition, etc. Second, the discussion on photodetectors based on 1D CdSe nanomaterials has been divided into three parts, including photodiodes, photoconductors, and phototransistors. Besides, some new mechanisms (such as enhancement effect of localized surface plasmon, optical quenching effect of photoconductivity, and piezo-phototronic effect), which can be utilized to enhance the performance of photodetectors, have also been elaborated. Finally, some major challenges and opportunities towards the practical integration and application of 1D CdSe nanomaterials in photodetectors have been discussed, which need to be further investigated in the future.
Electrochemical DNA Biosensors Based on Labeling with Nanoparticles
Authors: Kokkinos Christos
Published: September 2019

This work reviews the field of DNA biosensors based on electrochemical determination of nanoparticle labels. These labeling platforms contain the attachment of metal nanoparticles (NPs) or quantum dots (QDs) on the target DNA or on a biorecognition reporting probe. Following the development of DNA bioassay, the nanotags are oxidized to ions, which are determined by voltammetric methods, such as pulse voltammetry (PV) and stripping voltammetry (SV). The synergistic effects of NPs amplification (as each nanoprobe releases a large number of detectable ions) and the inherent sensitivity of voltammetric techniques (e.g., thanks to the preconcentration step of SV) leads to the construction of ultrasensitive, low cost, miniaturized, and integrated biodevices. This review focuses on accomplishments in DNA sensing using voltammetric determination of nanotags (such as gold and silver NPs, and Cd- and Pb-based QDs), includes published works on integrated three electrode biodevices and paper-based biosystems, and discusses strategies for multiplex DNA assays and signal enhancement procedures. Besides, this review mentions the electroactive NP synthesis procedures and their conjugation protocols with biomolecules that enable their function as labels in DNA electrochemical biosensors.
Porous Hybrid Nanofibers Comprising ZnSe/CoSe₂/Carbon with Uniformly Distributed Pores as Anodes for High-Performance Sodium-Ion Batteries
Authors: Jeong Sun Young , Cho Jung Sang
Published: September 2019

Well-designed porous structured bimetallic ZnSe/CoSe₂/carbon composite nanofibers with uniformly distributed pores were prepared as anodes for sodium-ion batteries by electrospinning and subsequent simple heat-treatment processes. Size-controlled polystyrene (PS) nanobeads in the electrospinning solution played a key role in the formation and uniform distribution of pores in the nanofiber structure, after the removal of selected PS nanobeads during the heat-treatment process. The porous ZnSe/CoSe₂/C composite nanofibers were able to release severe mechanical stress/strain during discharge–charge cycles, introduce larger contact area between the active materials and the electrolyte, and provide more active sites during cycling. The discharge capacity of porous ZnSe/CoSe2/C composite nanofibers at the 10,000th cycle was 297 mA h g−1, and the capacity retention measured from the second cycle was 81%. The final rate capacities of porous ZnSe/CoSe2/C composite nanofibers were 438, 377, 367, 348, 335, 323, and 303 mA h g−1 at current densities of 0.1, 0.5, 1, 3, 5, 7, and 10 A g−1, respectively. At the higher current densities of 10, 20, and 30 A g−1, the final rate capacities were 310, 222, and 141 mA h g−1, respectively.
Effects of a High-Protein/Moderate-Carbohydrate Diet on Appetite, Gut Peptides, and Endocannabinoids—A Preview Study
Authors: Tischmann Lea , Drummen Mathijs , Gatta-Cherifi Blandine , Raben Anne , Fogelholm Mikael , Hartmann Bolette , Holst Jens J. , Matias Isabelle , Cota Daniela , Mensink Ronald P. , Joris Peter J. , Westerterp-Plantenga Margriet S. , Adam Tanja C.
Published: September 2019

Favorable effects of a high-protein/moderate-carbohydrate (HP/MCHO) diet after weight loss on body weight management have been shown. To extend these findings, associations between perception of hunger and satiety with endocannabinoids, and with glucagon-like peptide-1 (GLP-1) and polypeptide YY (PYY) were assessed. At approximately 34 months after weight loss, 22 female and 16 male participants (mean age 64.5 ± 5.9 years; body mass index (BMI) 28.9 ± 3.9 kg/m2) completed a 48 h respiration chamber study. Participants were fed in energy balance with a HP/MCHO diet with 25%:45%:30% or a moderate-protein/high-carbohydrate (MP/HCHO) diet with 15%:55%:30% of energy from protein:carbohydrate:fat. Endocannabinoids and related compounds, relevant postprandial hormones (GLP-1, PYY), hunger, satiety, and ad libitum food intake were assessed. HP/MCHO versus MP/HCHO reduced hunger perception. The lower decremental area under the curve (dAUC) for hunger in the HP/MCHO diet (−56.6% compared to MP, p < 0.05) was associated with the higher AUC for 2-arachidonoylglycerol (2-AG) concentrations (p < 0.05). Hunger was inversely associated with PYY in the HP/MCHO group (r = −0.7, p < 0.01). Ad libitum food intake, homeostatic model assessment for insulin resistance (HOMA-IR) and incremental AUCs for gut peptides were not different between conditions. HP/MCHO versus MP/HCHO diet-induced reduction in hunger was present after 34 months weight maintenance in the post-obese state. HP/MCHO diet-induced decrease of hunger is suggested to interact with increased 2-AG and PYY concentrations.
A Novel Anti-Inflammatory Role of Omega-3 PUFAs in Prevention and Treatment of Atherosclerosis and Vascular Cognitive Impairment and Dementia
Authors: Simonetto Marialaura , Infante Marco , Sacco Ralph L. , Rundek Tatjana , Della-Morte David
Published: September 2019

Atherosclerosis is an inflammatory chronic disease affecting arterial vessels and leading to vascular diseases, such as stroke and myocardial infarction. The relationship between atherosclerosis and risk of neurodegeneration has been established, in particular with vascular cognitive impairment and dementia (VCID). Systemic atherosclerosis increases the risk of VCID by inducing cerebral infarction, or through systemic or local inflammatory factors that underlie both atherosclerosis and cognition. Omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) are involved in inflammatory processes, but with opposite roles. Specifically, omega-3 PUFAs exert anti-inflammatory properties by competing with omega-6 PUFAs and displacing arachidonic acid in membrane phospholipids, decreasing the production of pro-inflammatory eicosanoids. Experimental studies and some clinical trials have demonstrated that omega-3 PUFA supplementation may reduce the risk of different phenotypes of atherosclerosis and cardiovascular disease. This review describes the link between atherosclerosis, VCID and inflammation, as well as how omega-3 PUFA supplementation may be useful to prevent and treat inflammatory-related diseases.
Effect of Sodium Hypochlorite and Benzalkonium Chloride on the Structural Parameters of the Biofilms Formed by Ten Salmonella enterica Serotypes
Authors: Capita Rosa , Fernández-Pérez Silvia , Buzón-Durán Laura , Alonso-Calleja Carlos
Published: September 2019

The influence of the strain on the ability of Salmonella enterica to form biofilms on polystyrene was investigated by confocal laser scanning microscopy. The effects of sodium hypochlorite with 10% active chlorine (SHY; 25,000, 50,000, or 100,000 ppm), and benzalkonium chloride (BZK; 1000, 5000, or 10,000 ppm) on twenty-four-hour-old biofilms was also determined. The biofilms of ten Salmonella enterica isolates from poultry (S. Agona, S. Anatum, S. Enteritidis, S. Hadar, S. Infantis, S. Kentucky, S. Thompson, S. Typhimurium, monophasic variant of S. Typhimurium 1,4,(5),12:i:-, and S. Virchow) were studied. Biofilms produced by S. Anatum, S. Hadar, S. Kentucky, and S. Typhimurium showed a trend to have the largest biovolume and the greatest surface coverage and thickness. The smallest biofilms (P < 0.01) in the observation field (14.2 × 103 µm2) were produced by S. Enteritidis and S. 1,4,(5),12:i:- (average 12.9 × 103 ± 9.3 × 103 µm3) compared to the rest of the serotypes (44.4 × 103 ± 24.7 × 103 µm3). Biovolume and surface coverage decreased after exposure for ten minutes to SHY at 50,000 or 100,000 ppm and to BZK at 5000 or 10,000 ppm. However, the lowest concentrations of disinfectants increased biovolume and surface coverage in biofilms of several strains (markedly so in the case of BZK). The results from this study suggest that the use of biocides at low concentrations could represent a public health risk. Further research studies under practical field conditions should be appropriate to confirm these findings.
First Molecular Evidence of Anaplasma bovis and Anaplasma phagocytophilum in Bovine from Central Punjab, Pakistan
Authors: Iqbal Naveed , Mukhtar Muhammad Uzair , Yang Jifei , Sajid Muhammad Sohail , Niu Qingli , Guan Guiquan , Liu Zhijie , Yin Hong
Published: September 2019

Obligate intracellular bacteria belonging to the genus Anaplasma spp. are responsible for causing a hemolytic disease called anaplasmosis in animals, as well as in humans. This study was aimed at the molecular identification and genetic analysis of responsible causative agents of anaplasmosis beyond those already reported. A survey was performed during July and August 2018 in the Jhang District, Punjab, Pakistan. Four hundred and fifty blood samples from asymptomatic, tick-infested cattle were collected on FTA cards and tested for the Anaplasma spp. presence using nested-polymerase chain reaction (PCR) methods. The 16S ribosomal RNA gene sequences generated from the positive samples were used for genetic analysis of Anaplasma spp. The nested-PCR results showed the presence of two Anaplasma spp. with an overall prevalence rate of 10.44%, where the prevalence of A. bovis and A. phagocytophilum was 7.78% and 2.66%, respectively. The study portrayed new molecular data on the prevalence of Anaplasma spp. in the studied cattle population, indicating a potential threat to the human population as well.
Common Host Responses in Murine Aerosol Models of Infection Caused by Highly Virulent Gram-Negative Bacteria from the Genera Burkholderia, Francisella and Yersinia
Authors: Clark Graeme C. , Essex-Lopresti Angela , Moore Karen A. , Williamson E. Diane , Lukaszewski Roman , Paszkiewicz Konrad , David Jonathan
Published: September 2019

Highly virulent bacterial pathogens cause acute infections which are exceptionally difficult to treat with conventional antibiotic therapies alone. Understanding the chain of events that are triggered during an infection of a host has the potential to lead to new therapeutic strategies. For the first time, the transcriptomic responses within the lungs of Balb/C mice have been compared during an acute infection with the intracellular pathogens Burkholderia pseudomallei, Francisella tularensis and Yersinia pestis. Temporal changes were determined using RNAseq and a bioinformatics pipeline; expression of protein was also studied from the same sample. Collectively it was found that early transcriptomic responses within the infected host were associated with the (a) slowing down of critical cellular functions, (b) production of circulatory system components, (c) lung tissue integrity, and (d) intracellular regulatory processes. One common molecule was identified, Errfi1 (ErbB receptor feedback inhibitor 1); upregulated in response to all three pathogens and a potential novel marker of acute infection. Based upon the pro-inflammatory responses observed, we sought to synchronise each infection and report that 24 h p.i. of B. pseudomallei infection closely aligned with 48 h p.i. of infection with F. tularensis and Y. pestis. Post-transcriptional modulation of RANTES expression occurred across all pathogens, suggesting that these infections directly or indirectly modulate cell trafficking through chemokine expression/detection. Collectively, this unbiased NGS approach has provided an in-depth characterisation of the host transcriptome following infection with these highly virulent pathogens ultimately aiding in the development of host-directed therapies as adjuncts or alternatives to antibiotic treatment.
Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
Authors: Calata Fritz Ivy C. , Caranguian Camille Z. , Mendoza Jillian Ela M. , Fornillos Raffy Jay C. , Tabios Ian Kim B. , Fontanilla Ian Kendrich C. , Leonardo Lydia R. , Sunico Louie S. , Kawai Satoru , Chigusa Yuichi , Kikuchi Mihoko , Sato Megumi , Minamoto Toshifumi , Baoanan Zenaida G. , Sato Marcello Otake
Published: September 2019

Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. Methods: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. Results: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. Conclusion: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes.
Phosphonic Acid Analogues of Phenylglycine as Inhibitors of Aminopeptidases: Comparison of Porcine Aminopeptidase N, Bovine Leucine Aminopeptidase, Tomato Acidic Leucine Aminopeptidase and Aminopeptidase from Barley Seeds
Authors: Wanat Weronika , Talma Michał , Pawełczak Małgorzata , Kafarski Paweł
Published: September 2019

The inhibitory activity of 14 racemic phosphonic acid analogs of phenylglycine, substituted in aromatic rings, towards porcine aminopeptidase N (pAPN) and barley seed aminopeptidase was determined experimentally. The obtained patterns of the inhibitory activity against the two enzymes were similar. The obtained data served as a basis for studying the binding modes of these inhibitors by pAPN using molecular modeling. It was found that their aminophosphonate fragments were bound in a highly uniform manner and that the difference in their affinities most likely resulted from the mode of substitution of their phenyl rings. The obtained binding modes towards pAPN were compared, with these predicted for bovine lens leucine aminopeptidase (blLAP) and tomato acidic leucine aminopeptidase (tLAPA). The performed studies indicated that the binding manner of the phenylglycine analogs to biLAP and tLAPA are significantly similar and differ slightly from that predicted for pAPN.
Validation of Cadherin HAV6 Peptide in the Transient Modulation of the Blood-Brain Barrier for the Treatment of Brain Tumors
Authors: Sajesh Babu V. , On Ngoc H. , Omar Refaat , Alrushaid Samaa , Kopec Brian M. , Wang Wei-Guang , Sun Han-Dong , Lillico Ryan , Lakowski Ted M. , Siahaan Teruna J. , Davies Neal M. , Puno Pema-Tenzin , Vanan Magimairajan Issai , Miller Donald W.
Published: September 2019

The blood-brain barrier (BBB) poses a major obstacle by preventing potential therapeutic agents from reaching their intended brain targets at sufficient concentrations. While transient disruption of the BBB has been used to enhance chemotherapeutic efficacy in treating brain tumors, limitations in terms of magnitude and duration of BBB disruption exist. In the present study, the preliminary safety and efficacy profile of HAV6, a peptide that binds to the external domains of cadherin, to transiently open the BBB and improve the delivery of a therapeutic agent, was evaluated in a murine brain tumor model. Transient opening of the BBB in response to HAV6 peptide administration was quantitatively characterized using both a gadolinium magnetic resonance imaging (MRI) contrast agent and adenanthin (Ade), the intended therapeutic agent. The effects of HAV6 peptide on BBB integrity and the efficacy of concurrent administration of HAV6 peptide and the small molecule inhibitor, Ade, in the growth and progression of an orthotopic medulloblastoma mouse model using human D425 tumor cells was examined. Systemic administration of HAV6 peptide caused transient, reversible disruption of BBB in mice. Increases in BBB permeability produced by HAV6 were rapid in onset and observed in all regions of the brain examined. Concurrent administration of HAV6 peptide with Ade, a BBB impermeable inhibitor of Peroxiredoxin-1, caused reduced tumor growth and increased survival in mice bearing medulloblastoma. The rapid onset and transient nature of the BBB modulation produced with the HAV6 peptide along with its uniform disruption and biocompatibility is well-suited for CNS drug delivery applications, especially in the treatment of brain tumors.
The Associations between Leaf Morphology, Phenylalanine Ammonia Lyase Activity, Reactive Oxygen Species, and Fusarium Resistance in Selected Species of Wheat with Different Ploidy Levels
Authors: Duba Adrian , Goriewa-Duba Klaudia , Wachowska Urszula , Głowacka Katarzyna , Wiwart Marian
Published: September 2019

In wheat, resistance to Fusarium is conditioned by anatomical, morphological, and physiological traits. The aim of this study was to evaluate selected elements of constitutive barriers in common wheat, spelt, Polish wheat, emmer, and einkorn. The activity of the phenylalanine ammonia-lyase (PAL) enzyme and rate of reactive oxygen species (ROS) production were evaluated in the tissues of common wheat and spelt inoculated with Fusarium culmorum. Most of the relict wheat species were more abundant in morphological barriers than common wheat. F. culmorum penetrated constitutive barriers, which increased PAL activity and intensified ROS production 24 h after inoculation in wheat tissues. The lowest increase in PAL activity after inoculation was observed in cv. Sumai3, which resistance is based on limiting the spread of F. culmorum within the spike. Spelt line Tas 581 glumes were characterized by the highest concentration of ROS 24 h after inoculation. The ROS content remained high for five days. The results of this study indicate that high trichome density plays a key role in resistance to pathogens. In the resistant spelt line with effective constitutive barriers, PAL activity and ROS content were higher than those observed in susceptible wheats after inoculation with F. culmorum.
Dynamics of Short-Term Metabolic Profiling in Radish Sprouts (Raphanus sativus L.) in Response to Nitrogen Deficiency
Authors: Baek Seung-A , Im Kyung-Hoan , Park Sang Un , Oh Sung-Dug , Choi Jaehyuk , Kim Jae Kwang
Published: September 2019

Nitrogen (N) is a macronutrient important for the survival of plants. To investigate the effects of N deficiency, a time-course metabolic profiling of radish sprouts was performed. A total of 81 metabolites—including organic acids, inorganic acid, amino acids, sugars, sugar alcohols, amines, amide, sugar phosphates, policosanols, tocopherols, phytosterols, carotenoids, chlorophylls, and glucosinolates—were characterized. Principal component analysis and heat map showed distinction between samples grown under different N conditions, as well as with time. Using PathVisio, metabolic shift in biosynthetic pathways was visualized using the metabolite data obtained for 7 days. The amino acids associated with glucosinolates accumulated as an immediate response against –N condition. The synthesis of pigments and glucosinolates was decreased, but monosaccharides and γ-tocopherol were increased as antioxidants in radish sprouts grown in –N condition. These results indicate that in radish sprouts, response to N deficiency occurred quickly and dynamically. Thus, this metabolic phenotype reveals that radish responds quickly to N deficiency by increasing the content of soluble sugars and γ-tocopherol, which acts as a defense mechanism after the germination of radish seeds.
Alleviation of Phytophthora infestans Mediated Necrotic Stress in the Transgenic Potato (Solanum tuberosum L.) with Enhanced Ascorbic acid Accumulation
Authors: Chung Ill-Min , Venkidasamy Baskar , Upadhyaya Chandrama Prakash , Packiaraj Gurusaravanan , Rajakumar Govindasamy , Thiruvengadam Muthu
Published: September 2019

Potato is the most widely cultivated non-cereal crop in the world, and like any other crop, it is susceptible to yield losses because of various factors, including pathogen attacks. Among the various diseases of potato, late blight caused by the oomycete Phytophthora infestans is considered as the most devastating disease worldwide. In this study, transgenic potato plants overexpressing the D-galacturonic acid reductase (GalUR) gene with an enhanced level of cellular L-ascorbate (L-AsA) were challenged with Phytophthora infestans to determine the level of stress tolerance induced in those plants. With the onset of pathogen infection, necrotic lesions progressively expanded and became necrotic in the control plants. The transgenic potato lines with enhanced ascorbic acid showed reduced necrotic lesions. Hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels were relatively lower in transgenic plants compared to the untransformed control (UT) plants. The mRNA expressions of pathogenesis-related (PR) genes, such as pathogenesis related 1 (PR1) and phenylalanine ammonia-lyase (PAL) were slightly higher in GalUR overexpressing transgenic lines as compared to the untransformed control plants. Pathogen infection also altered the mRNA expression of genes associated with gibberellic acid (GA) and abscisic acid (ABA) biosynthesis. Furthermore, the increase in various antioxidant enzymes was also observed in the gene expression analysis with the transgenic plants. The complete loss of the pathogen growth and disease occurrence was not observed in our study; however, the findings indicated that an increase in the level of cellular L-ascorbate in the transgenic potato leads to enhanced cellular antioxidants, PR genes and plant defense hormones, such as GA and ABA resulting in the reduction of the disease symptoms caused by the Phytophthora infestans.
Exogenous Melatonin Delays Dark-Induced Grape Leaf Senescence by Regulation of Antioxidant System and Senescence Associated Genes (SAGs)
Authors: Shi Xingyun , Xu Shanshan , Mu Desheng , Sadeghnezhad Ehsan , Li Qiang , Ma Zonghuan , Zhao Lianxin , Zhang Qinde , Wang Lixin
Published: September 2019

Leaf senescence is a developmentally programmed and degenerative process which comprises the last stage of the life cycle of leaves. In order to understand the melatonin effect on grapevine leaf senescence, the dark treatment on detached leaves of Vitis vinifera L. cv. Red Globe was performed to induce leaf senescence at short period of time. Then, a series of physiological and molecular changes in response to exogenous melatonin were measured. Results showed that 100 μM of melatonin treatment could significantly delay the dark induced leaf senescence, which is accompanied by the decreased production of reactive oxygen species (ROS). Meanwhile, melatonin treatment could increase the scavenging activity of antioxidant enzymes, such as peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). Simultaneously, ascorbate (AsA) and glutathione (GSH) contents, the activities of ascorbate peroxidase (APX), and glutathione reductase (GR) were significantly higher than control treatment in samples treated with melatonin. Furthermore, melatonin treatment showed to suppress the expression of leaf senescence-associated genes (SAGs). All these results demonstrated that melatonin could activate the antioxidant and Ascorbate-Glutathione (AsA-GSH) cycle system and repress the expression of SAGs that lead to delay the dark induced grape leaf senescence.
Preparation of a Novel Fracturing Fluid System with Excellent Elasticity and Low Friction
Authors: Zhang Yang , Mao Jincheng , Zhao Jinzhou , Xu Tao , Du Anqi , Zhang Zhaoyang , Zhang Wenlong , Ma Shaoyun
Published: September 2019

The quaternary polymer was synthesized by radical polymerization and characterized by 1H NMR. The tests of critical associating concentration and SEM suggest that there is a multilayered and robust network structure in the polymer solution. An excellent elasticity in the polymer solution by the first normal stress difference, viscoelasticity, and thixotropy was observed. The critical crosslinker concentration of polymer with sodium dodecyl sulfate and its interaction mechanism were investigated. According to the reaction kinetics, the supramolecular structure had the lowest activation energy, stable network structure, and greater thermal stability. Then the polymer was employed in the fracturing fluid due to its excellent elasticity using the intermolecular forces, which showed superior sand suspension capacity by dynamic sand suspension measurement. Meanwhile, a theoretical analysis was proposed as to why polymer solution has excellent suspension and drag reduction properties. Therefore, this polymer could be an alternative in many fields, especially in fracking, which is significant for the development of oil and gas resources in deep wells.
Effects of Single-arc Blade Profile Length on the Performance of a Forward Multiblade Fan
Authors: Wei Yikun , Ying Cunlie , Xu Jun , Cao Wenbin , Wang Zhengdao , Zhu Zuchao
Published: September 2019

The effects of single-arc blade profile length on the performance of a forward multiblade fan are investigated in this paper by computational fluid dynamics and experimental measurement. The present work emphasizes that the use of a properly reduced blade inlet angle (β1A) and properly improved blade outlet angle (β2A) is to increase the length blade profile, which suggests a good physical understanding of internal complex flow characteristics and the aerodynamic performance of the fan. Numerical results indicate that the gradient of the absolute velocity among the blades in model-L (reducing the blade inlet angle and improving blade outlet angle) is clearly lower than that of the baseline model and model-S (improving the blade inlet angle and reducing blade outlet angle), where a number of secondary flows arise on the exit surface of baseline model and model-S. However, no secondary flow occurs in model-L, and the flow loss at the exit surface of the volute (scroll-shaped flow patterns) for model-L is obviously lower than that of the baseline model at the design point. The comparison of the test results further shows that to improve the blade profile length is to increase the static pressure and the efficiency of the static pressure, since the improved static pressure of the model-L rises as much as 22.5 Pa and 26.2%, and the improved static pressure efficiency of the model-L rises as much as 5 % at the design flow rates. It is further indicated that increasing the blade working area provides significant physical insight into increasing the static pressure, total pressure, the efficiency of the static pressure and the total pressure efficiency.
Mild Hydrothermal Pretreatment of Microalgae for the Production of Biocrude with a Low N and O Content
Authors: Montero-Hidalgo Miriam , Espada Juan J. , Rodríguez Rosalía , Morales Victoria , Bautista Luis Fernando , Vicente Gemma
Published: September 2019

A hydrothermal pretreatment of the microalga Nannochloropsis gaditana at mild temperatures was studied in order to reduce the N and O content in the biocrude obtained by hydrothermal liquefaction (HTL). The work focused on the evaluation of temperature, reactor loading, and time (factors) to maximize the yield of the pretreated biomass and the heteroatom contents transferred from the microalga biomass to the aqueous phase (responses). The study followed the factorial design and response surface methodology. An equation for every response was obtained, which led to the accurate calculation of the operating conditions required to obtain a given value of these responses. Temperature and time are critical factors with a negative effect on the pretreated biomass yield but a positive one on the N and O recovery in the aqueous phase. The slurry concentration has to be low to increase heteroatom recovery and has to be high to maximize the pretreated microalga yields. Response equations were obtained for the analyzed responses, which facilitated the accurate prediction of the operating conditions required to obtain a given value of these responses.
Effect of Cellulosic Waste Derived Filler on the Biodegradation and Thermal Properties of HDPE and PLA Composites
Authors: Quitadamo Alessia , Massardier Valerie , Iovine Valeria , Belhadj Ahmed , Bayard Rémy , Valente Marco
Published: September 2019

Composites with high density polyethylene (HDPE) and poly(lactic) acid (PLA) matrix have been tested to analyze the effect of natural fillers (wood flour, recycled wastepaper and a mix of both fillers) and temperature on polymer degradation. Composting tests have been performed in both mesophilic (35 °C) and thermophilic (58 °C) conditions. Degradation development has been evaluated through mass variation, thermogravimetric analysis and differential scanning calorimetry. HDPE, as expected, did not display any relevant variation, confirming its stability under our composting conditions. PLA is sensibly influenced by temperature and humidity, with higher reduction of Mw when composting is performed at 58 °C. Natural fillers seem to influence degradation process of composites, already at 35 °C. In fact, degradation of fillers at 35 °C allows a mass reduction during composting of composites, while neat PLA do not display any variation.
Experimental and Numerical Simulation Study on Co-Incineration of Solid and Liquid Wastes for Green Production of Pesticides
Authors: Zhang Bin , He Jinjie , Hu Chengming , Chen Wei
Published: September 2019

A large amount of solid and liquid waste is produced in pesticide production. It is necessary to adopt appropriate disposal processes to reduce pollutant emissions. A co-incineration scheme for mixing multi-component wastes in a rotary kiln was proposed for waste disposal from pesticide production. According to the daily output of solid and liquid wastes, the proportion of mixing was determined. An experiment of the co-incineration of solid and liquid wastes was established. Experimental results showed that the mixed waste could be completely disposed at 850 °C, and the residence time in the kiln exceeded 1 h. A model method for mixture and diesel oil-assisted combustion was proposed. Numerical simulation was performed to predict the granular motion and reveal the combustion interactions of the co-incineration of mixed wastes in the rotary kiln. Simulation results reproduced movements, such as rolling and cascading, and obtained the optimum rotational speed and diesel oil flow for the rotary kiln incineration operation. The simulation showed that the temperature in the kiln was maintained at 850 °C, and the mass fraction of CO and O2 at the outlet reached the standard for the complete combustion of the waste. Finally, the rotary kiln incineration and flue gas treatment processes were successfully applied in engineering for green production of pesticides.
Bioenvironmental Zonal Controlling of Incubated Avian Embryo Using Localised Infrared Heating
Authors: Youssef Ali , Norton Tomas , Berckmans Daniel
Published: September 2019

The main objective of any bioenvironmental controller is to create favourable bioenvironmental conditions around the living-system. In industrial incubation practice of chicken embryo, it is sometimes difficult to fill large incubators with uniform eggs, which leads to suboptimal results. The ideal incubation solution is a machine that is capable of coping with all sorts of variabilities in eggs. This can be realised in practice by creating different zones of different environmental conditions within the same machine. In the present study, a two-levels controller was designed and implemented to combine both convective and radiative heating to incubate eggs. On the higher level, three model-predictive-control (MPC) constrained controllers were developed to regulate the power applied to nine IR-radiators divided into three zones based on continuous feedback of the eggshell temperatures in each zone. On the lower level, a PID controller was used to maintain the air temperature within an experimental incubator at a fixed level (34 °C) lower than the standard incubation temperature. Four full incubation trials were carried out to test and implement the developed zonal controllers. The implementation results showed that the developed controllers were able to follow the reference trajectory defined for each zone. It was possible to keep the eggshell temperatures within the middle region (zone) different from the sidelong regions (zones) while the air temperature kept constant at 34 °C. The average hatching result (HOF) of the four full incubation trial was 84.0% (±0.5). The developed two-levels control system is a promising technique for demand-based climate controller and to optimizing energy use by using multi-objectives MPCs with constraint on total energy consumption.
In Silico Identification of Antimicrobial Peptides in the Proteomes of Goat and Sheep Milk and Feta Cheese
Authors: Tomazou Marios , Oulas Anastasis , Anagnostopoulos Athanasios K. , Tsangaris George Th. , Spyrou George M.
Published: September 2019

Milk and dairy products are a major functional food group of growing scientific and commercial interest due to their nutritional value and bioactive “load”. A major fraction of the latter is attributed to milk’s rich protein content and its biofunctional peptides that occur naturally during digestion. On the basis of the identified proteome datasets of milk whey from sheep and goat breeds in Greece and feta cheese obtained during previous work, we applied an in silico workflow to predict and characterise the antimicrobial peptide content of these proteomes. We utilised existing tools for predicting peptide sequences with antimicrobial traits complemented by in silico protein cleavage modelling to identify frequently occurring antimicrobial peptides (AMPs) in the gastrointestinal (GI) tract in humans. The peptides of interest were finally assessed for their stability with respect to their susceptibility to cleavage by endogenous proteases expressed along the intestinal part of the GI tract and ranked with respect to both their antimicrobial and stability scores.
A Hierarchical Airport Detection Method Using Spatial Analysis and Deep Learning
Authors: Zeng Fanxuan , Cheng Liang , Li Ning , Xia Nan , Ma Lei , Zhou Xiao , Li Manchun
Published: September 2019

Airports have a profound impact on our lives, and uncovering their distribution around the world has great significance for research and development. However, existing airport databases are incomplete and have a high cost of updating. Thus, a fast and automatic worldwide airport detection method can be of significance for global airport detection at regular intervals. However, previous airport detection studies are usually based on single remote sensing (RS) imagery, which seems an overwhelming burden for worldwide airport detection with traversal searching. Thus, we propose a hierarchical airport detection method consisting of broad-scale extraction of worldwide candidate airport regions based on spatial analysis of released RS products, including impervious surfaces from FROM-GLC10 (fine resolution observation and monitoring of global land cover 10) product, building distribution from OSMs (open street maps) and digital surface model from AW3D30 (ALOS World 3D—30 m). Moreover, narrow-scale aircraft detection was initially conducted by the Faster R-CNN (regional-convolutional neural networks) deep learning method. To avoid overestimation of background regions by Faster R-CNN, a second CNN classifier is used to refine the class labeling with negative samples. Specifically, our research focuses on target airports with at least 2 km length in three experimental regions. Results show that spatial analysis reduced the possible regions to 0.56% of the total area of 75,691 km2. The initial aircraft detection by Faster R-CNN had a mean user’s accuracy of 88.90% and ensured that all the aircrafts could be detected. Then, by introducing the CNN reclassifier, the user’s accuracy of aircraft detection was significantly increased to 94.21%. Finally, through an experienced threshold of aircraft number, 19 of the total 20 airports were detected correctly. Our results reveal the overall workflow is reliable for automatic and rapid airport detection around the world with the help of released RS products. This research promotes the application and progression of deep learning.
Height Variation Depending on the Source of Antenna Phase Centre Corrections: LEIAR25.R3 Case Study
Authors: Araszkiewicz Andrzej , Kiliszek Damian , Podkowa Anna
Published: September 2019

In this study, we compared two sets of antenna phase center corrections for groups of the same type of antenna mounted at the continuously operating global navigation satellite system (GNSS) reference stations. The first set involved type mean models provided by the International GNSS Service (release igs08), while the second set involved individual models developed by Geo++. Our goal was to check which set gave better results in the case of height estimation. The paper presents the differences between models and their impact on resulting height. Analyses showed that, in terms of the stability of the determined height, as well as its variability caused by increasing the facade mask, both models gave very similar results. Finally, we present a method for how to estimate the impact of differences in phase center corrections on height changes.
An FPGA-Based Neuro-Fuzzy Sensor for Personalized Driving Assistance
Authors: Mata-Carballeira Óscar , Gutiérrez-Zaballa Jon , del Campo Inés , Martínez Victoria
Published: September 2019

Advanced driving-assistance systems (ADAS) are intended to automatize driver tasks, as well as improve driving and vehicle safety. This work proposes an intelligent neuro-fuzzy sensor for driving style (DS) recognition, suitable for ADAS enhancement. The development of the driving style intelligent sensor uses naturalistic driving data from the SHRP2 study, which includes data from a CAN bus, inertial measurement unit, and front radar. The system has been successfully implemented using a field-programmable gate array (FPGA) device of the Xilinx Zynq programmable system-on-chip (PSoC). It can mimic the typical timing parameters of a group of drivers as well as tune these typical parameters to model individual DSs. The neuro-fuzzy intelligent sensor provides high-speed real-time active ADAS implementation and is able to personalize its behavior into safe margins without driver intervention. In particular, the personalization procedure of the time headway (THW) parameter for an ACC in steady car following was developed, achieving a performance of 0.53 microseconds. This performance fulfilled the requirements of cutting-edge active ADAS specifications.
On-Board Real-Time Trajectory Planning for Fixed Wing Unmanned Aerial Vehicles in Extreme Environments
Authors: Schellenberg Ben , Richardson Tom , Richards Arthur , Clarke Robert , Watson Matt
Published: September 2019

A team from the University of Bristol have developed a method of operating fixed wing Unmanned Aerial Vehicles (UAVs) at long-range and high-altitude over Volcán de Fuego in Guatemala for the purposes of volcanic monitoring and ash-sampling. Conventionally, the mission plans must be carefully designed prior to flight, to cope with altitude gains in excess of 3000 m, reaching 9 km from the ground control station and 4500 m above mean sea level. This means the climb route cannot be modified mid-flight. At these scales, atmospheric conditions change over the course of a flight and so a real-time trajectory planner (RTTP) is desirable, calculating a route on-board the aircraft. This paper presents an RTTP based around a genetic algorithm optimisation running on a Raspberry Pi 3 B+, the first of its kind to be flown on-board a UAV. Four flights are presented, each having calculated a new and valid trajectory on-board, from the ground control station to the summit region of Volcań de Fuego. The RTTP flights are shown to have approximately equivalent efficiency characteristics to conventionally planned missions. This technology is promising for the future of long-range UAV operations and further development is likely to see significant energy and efficiency savings.