bluuebunny/crossref_metadata_2025 huggingface dataset:
Comprehensive Transcriptome Analysis Revealed the Effects of the Light Quality, Light Intensity, and Photoperiod on Phlorizin Accumulation in Lithocarpus polystachyus Rehd.
Authors: Zhang Yantong , Lin Limei , Long Yuehong , Guo Hongyu , Wang Zhuo , Cui Minghui , Huang Jian , Xing Zhaobin
Published: November 2019

Lithocarpus polystachyus Rehd. is an important medicinal plant species grown in southern China, with phlorizin as its main active substance. The effects of light conditions on phlorizin biosynthesis in L. polystachyus remain unclear. Thus, we analyzed the transcriptomes of L. polystachyus plants cultivated under diverse light qualities, light intensities, and photoperiods. The light treatments resulted in 5977–8027 differentially expressed genes (DEGs), which were functionally annotated based on the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Genes encoding transcription factors from 89 families were differentially expressed after the light treatments, implying these transcription factors are photoresponsive. Phenylalanine ammonia lyase (PAL) and 4-coumarate-CoA ligase (4CL) are the key enzymes for the accumulation of phlorizin. The transcription levels of PAL2, PAL, 4CL1 (DN121614), 4CLL7, and 4CL1 (DN102161) were positively correlated with phlorizin accumulation, suggesting that these genes are important for phlorizin biosynthesis. An ultra-high-performance liquid chromatography method was used to quantify the phlorizin content. Phlorizin accumulated in response to the green light treatment and following appropriate decreases in the light intensity or appropriate increases in the duration of the light exposure. The green light, 2000 lx, and 3000 lx treatments increased the PAL activity of L. polystachyus, but the regulatory effects of the light intensity treatments on PAL activity were relatively weak. This study represents the first comprehensive analysis of the light-induced transcriptome of L. polystachyus. The study results may form the basis of future studies aimed at elucidating the molecular mechanism underlying phlorizin biosynthesis in L. polystachyus. Moreover, this study may be relevant for clarifying the regulatory effects of light on the abundance of bioactive components in medicinal plants.
Temperature, Wind, Cloud, and the Postglacial Tree Line History of Sub-Antarctic Campbell Island
Authors: McGlone Matt S. , Wilmshurst Janet M. , Richardson Sarah J. , Turney Chris S.M. , Wood Jamie R.
Published: November 2019

Campbell Island, which is 600 km south of New Zealand, has the southernmost tree line in this ocean sector. Directly under the maximum of the westerlies, the island is sensitive to changes in wind strength and direction. Pollen records from three peat cores spanning the tree line ecotone provide a 17,000-year history of vegetation change, temperature, and site moisture. With postglacial warming, tundra was replaced by tussock grassland 12,500 years ago. A subsequent increase of shrubland was reversed at 10,500 years ago and wetland-grassland communities became dominant. Around 9000 years ago, trees spread, with maximum tree line elevation reached around 6500 to 3000 years ago. This sequence is out of step with Southern Ocean sea surface temperatures, which were warmer than 12,500 to 9000 years ago, and, subsequently, cooled. Campbell Island tree lines were decoupled from temperature trends in the adjacent ocean by weaker westerlies from 12,500 to 9000 years ago, which leads to the intrusion of warmer, cloudier northern airmasses. This reduced solar radiation and evapotranspiration while increasing atmospheric humidity and substrate wetness, which suppressed tree growth. Cooler, stronger westerlies in the Holocene brought clearer skies, drier air, increased evapotranspiration, and rising tree lines. Future global warming will not necessarily lead to rising tree lines in oceanic regions.
Development and Application of a New QuEChERS Method in UHPLC-QqQ-MS/MS to Detect Seven Biogenic Amines in Chinese Wines
Authors: Han Shun-Yu , Hao Lan-Lan , Shi Xiao , Niu Jian-Ming , Zhang Bo
Published: November 2019

The aim of this study was to develop and validate an improved, simple, and sensitive method for the simultaneous determination of seven types (cadaverine, CAD; hexylamine, HEX; histamine, HIS; phenylethylamine, PEA; putrescine, PUT; tyramine, TYR) of biogenic amines (BAs) in wine matrices. For this reason, a modified QuEChERS combined with ultra-performance liquid chromatography coupled to a triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) method was investigated. The optimization of UHPLC-QqQ-MS/MS separation and QuEChERS procedure was performed. Under optimum conditions, the excellent chromatographic performance of the whole separation was accomplished within 6.3 min analyzing time. Meanwhile, the recoveries ranged from 77.2% to 101.7%, while relative standard deviation (RSD) remained between 0.0% and 9.4%. The limit of detection (LOD, 0.50–1.00 µg/L) and the limit of quantification (LOQ, 1.65–3.30 µg/L) were lower than those permitted by legislation in food matrices, which demonstrated the high sensitivity and applicability of this efficient method. This validated method was also applied in a pilot study to analyze BAs in 81 wine samples from Hexi Corridor Region (Gansu Province, Northwest China), CAD, HEX, HIS, PEA, PUT, and TYR were detected to varying degrees in the samples. However, when compared with the existing standards, the BAs in all 81 wine samples did not exceed the prescribed limit value or toxic dose (2–40 mg/L). Moreover, a statistical approach was also conducted using Pearson correlation analysis, and to evaluate their concentrations in terms of wine parameters (storage time, grape variety, wine type, and basic physicochemical index). The results showed that, among the seven kinds of BAs, the concentration of HIS had a certain correlation with alcoholic degree and grape variety. In addition, the level of PEA had a certain correlation with the wine pH and wine storage time. It is worth noting that this seems to be the first report regarding the application of QuEChERS-UHPLC-QqQ-MS/MS in the analysis of BAs in wine in this region.
Medium Chain Triglyceride (MCT) Oil Affects the Immunophenotype via Reprogramming of Mitochondrial Respiration in Murine Macrophages
Authors: Yu Seungmin , Go Gwang-woong , Kim Wooki
Published: November 2019

Medium chain triglyceride (MCT) oil has been postulated to modulate inflammatory responses, but the detailed mechanisms have not been fully elucidated. Based on recent studies demonstrating that mitochondrial metabolic reprogramming and immune responses are correlated, the current study sought to determine whether MCT oil controls inflammatory responses through modulation of mitochondria using both in vitro and in vivo models. The mitochondrial metabolic phenotypes of macrophages were assessed according to oxygen consumption rate (OCR). Inflammatory responses were assessed for production of cytokines and expression of activation markers. MCT oil was more rapidly oxidized as observed by increased OCR in macrophages. The production of pro-inflammatory cytokines was down-regulated and anti-inflammatory cytokine was elevated by MCT oil. In addition, classically activated M1 and alternatively activated M2 markers were reciprocally regulated by MCT intervention. Overall, up-regulated β-oxidation by MCT contributes to the anti-inflammatory M2-like status of macrophages, which may aid in the dietary prevention and/or amelioration of inflammation.
Isolation of Penicillium citrinum from Roots of Clerodendron cyrtophyllum and Application in Biosynthesis of Aglycone Isoflavones from Soybean Waste Fermentation
Authors: Doan Duy , Luu Duc , Nguyen Thanh , Hoang Thi Bich , Pham Thi Hong , Do Huu , Luu Van , Pham The , Than Van , Pham Thi Hai , Pham Minh , Tran Quoc
Published: November 2019

Soybeans offer an abundant source of isoflavones, which confer useful bioactivities when existing in aglycone forms. The conversion of isoflavones into aglycones via fermentation of soybean products is often realized by β-glucosidase, an enzyme produced by fungi. In this study, a filamentous fungus, Clerodendron cyrtophyllum, was isolated from root of Clerodendron cyrtophyllum Turcz, which was able to produce the highest activity of β-glucosidase up to 33.72 U/mL at 144 h during fermentation on Potato Dextrose Broth (PDB). The obtained fungus was grown on isoflavones-rich soybean extract to produce genistein and daidzein, achieving the conversion rate of 98.7%. Genistein and daidzein were isolated and purified by column chromatography using hexane/acetone (29:1/1:1), reaching purities of over 90% of total isoflavones, as identified and determined by TLC, LC-MS/MS, and 1H and 13C NMR spectroscopy. These results imply that the isolated P. citrinum is a potential fungal strain for industrial-scale production of genistein and daidzein from isoflavones-containing soybean extracts. These products may serve as potential raw materials for manufacture of functional foods that are based on aglycones.
Rapid Estimation of Parameters for Gelatinization of Waxy Corn Starch
Authors: Bertrand Robert , Holmes William , Orgeron Cory , McIntyre Carl , Hernandez Rafael , Revellame Emmanuel D.
Published: November 2019

Starch gelatinization is an important process due to the prevalence of starch usage in industries such as cosmetics and food production. In this study, the gelatinization of waxy corn starch (WCS) was investigated with the goal of providing an option for the rapid determination of starch gelatinization characteristics. The procedure used in the study was solely based on differential scanning calorimetry (DSC), which is an established technique for the determination of thermal characteristics of starches. A sequence of experiments was conducted to determine the excess water condition, an estimate of the minimum gelatinization temperature, and gelatinization time. These parameters were found to be ≥65 wt.% water, 75–85 °C, and 10 min, respectively. The estimation of the minimum gelatinization temperature was determined from the thermal properties of the WCS as obtained by DSC. The obtained parameters resulted in complete WCS gelatinization, and, thus, the sequence of procedures used in the study could possibly be used for rapid waxy starch evaluation.
Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells
Authors: Oliviero Francesca , Zamudio-Cuevas Yessica , Belluzzi Elisa , Andretto Lisa , Scanu Anna , Favero Marta , Ramonda Roberta , Ravagnan Giampietro , López-Reyes Alberto , Spinella Paolo , Punzi Leonardo
Published: November 2019

Resveratol (RES) and its natural precursor polydatin (PD) are polyphenols that may display a broad variety of beneficial effects including anti-inflammatory properties. This study aimed to investigate the role of RES and PD in the inflammatory process induced by monosodium urate (MSU) and calcium pyrophosphate (CPP) crystals in vitro. A monocytic cell line (THP-1) was primed for 3 hours with phorbol myristate acetate (100 ng/mL) and stimulated with synthetic MSU (0.05 mg/mL) and CPP (0.025 mg/mL) crystals. RES and PD were added to cultures concurrently with the crystals, or as 2-hour pretreatment. The effect of the two polyphenols was evaluated on intracellular and extracellular IL-1β levels, NACHT-LRRPYD-containing protein-3 (NLRP3) inflammasome expression, reactive oxygen species (ROS) and nitric oxide (NO) production, and the assessment of crystal phagocytosis. RES and PD strongly inhibited IL-1β induced by crystals after cell pretreatment. Cell pretreatment was effective also in reducing IL-1 mRNA expression while no effect was observed on NLRP3 gene expression. RES and PD had no effect on crystal phagocytosis when used as pretreatment. Both polyphenols were significantly effective in inhibiting ROS and NO production. Our results demonstrated that RES and PD are effective in inhibiting crystal-induced inflammation. Data obtained after cell pretreatment allow us to hypothesize that these polyphenols act on specific signaling pathways, preventing inflammation.
Non-Coding RNA and Tumor Development in Neurofibromatosis Type 1: ANRIL Rs2151280 Is Associated with Optic Glioma Development and a Mild Phenotype in Neurofibromatosis Type 1 Patients
Authors: Tritto Viviana , Ferrari Luca , Esposito Silvia , Zuccotti Paola , Bianchessi Donatella , Natacci Federica , Saletti Veronica , Eoli Marica , Riva Paola
Published: November 2019

Non-coding RNAs (ncRNAs) are known to regulate gene expression at the transcriptional and post-transcriptional levels, chromatin remodeling, and signal transduction. The identification of different species of ncRNAs, microRNAs (miRNAs), circular RNAs (circRNAs), and long ncRNAs (lncRNAs)—and in some cases, their combined regulatory function on specific target genes—may help to elucidate their role in biological processes. NcRNAs’ deregulation has an impact on the impairment of physiological programs, driving cells in cancer development. We here carried out a review of literature concerning the implication of ncRNAs on tumor development in neurofibromatosis type 1 (NF1), an inherited tumor predisposition syndrome. A number of miRNAs and a lncRNA has been implicated in NF1-associated tumors, such as malignant peripheral nerve sheath tumors (MPNSTs) and astrocytoma, as well as in the pathognomonic neurofibromas. Some authors reported that the lncRNA ANRIL was deregulated in the blood of NF1 patients with plexiform neurofibromas (PNFs), even if its role should be further elucidated. We here provided original data concerning the association of a specific genotype about ANRIL rs2151280 with the presence of optic gliomas and a mild expression of the NF1 phenotype. We also detected the LOH of ANRIL in different tumors from NF1 patients, supporting the involvement of ANRIL in some NF1-associated tumors. Our results suggest that ANRIL rs2151280 may be a potential diagnostic and prognostic marker, addressing early diagnosis of optic glioma and predicting the phenotype severity in NF1 patients.
Evolution of Codon Usage Bias in Diatoms
Authors: Krasovec Marc , Filatov Dmitry A.
Published: November 2019

Codon usage bias (CUB)—preferential use of one of the synonymous codons, has been described in a wide range of organisms from bacteria to mammals, but it has not yet been studied in marine phytoplankton. CUB is thought to be caused by weak selection for translational accuracy and efficiency. Weak selection can overpower genetic drift only in species with large effective population sizes, such as Drosophila that has relatively strong CUB, while organisms with smaller population sizes (e.g., mammals) have weak CUB. Marine plankton species tend to have extremely large populations, suggesting that CUB should be very strong. Here we test this prediction and describe the patterns of codon usage in a wide range of diatom species belonging to 35 genera from 4 classes. We report that most of the diatom species studied have surprisingly modest CUB (mean Effective Number of Codons, ENC = 56), with some exceptions showing stronger codon bias (ENC = 44). Modest codon bias in most studied diatom species may reflect extreme disparity between astronomically large census and modest effective population size (Ne), with fluctuations in population size and linked selection limiting long-term Ne and rendering selection for optimal codons less efficient. For example, genetic diversity (pi ~0.02 at silent sites) in Skeletonema marinoi corresponds to Ne of about 10 million individuals, which is likely many orders of magnitude lower than its census size. Still, Ne ~107 should be large enough to make selection for optimal codons efficient. Thus, we propose that an alternative process—frequent changes of preferred codons, may be a more plausible reason for low CUB despite highly efficient selection for preferred codons in diatom populations. The shifts in the set of optimal codons should result in the changes of the direction of selection for codon usage, so the actual codon usage never catches up with the moving target of the optimal set of codons and the species never develop strong CUB. Indeed, we detected strong shifts in preferential codon usage within some diatom genera, with switches between preferentially GC-rich and AT-rich 3rd codon positions (GC3). For example, GC3 ranges from 0.6 to 1 in most Chaetoceros species, while for Chaetoceros dichaeta GC3 = 0.1. Both variation in selection intensity and mutation spectrum may drive such shifts in codon usage and limit the observed CUB. Our study represents the first genome-wide analysis of CUB in diatoms and the first such analysis for a major phytoplankton group.
Repression of Inappropriate Gene Expression in the Vertebrate Embryonic Ectoderm
Authors: Reich Shoshana , Weinstein Daniel C.
Published: November 2019

During vertebrate embryogenesis, precise regulation of gene expression is crucial for proper cell fate determination. Much of what we know about vertebrate development has been gleaned from experiments performed on embryos of the amphibian Xenopus laevis; this review will focus primarily on studies of this model organism. An early critical step during vertebrate development is the formation of the three primary germ layers—ectoderm, mesoderm, and endoderm—which emerge during the process of gastrulation. While much attention has been focused on the induction of mesoderm and endoderm, it has become clear that differentiation of the ectoderm involves more than the simple absence of inductive cues; rather, it additionally requires the inhibition of mesendoderm-promoting genes. This review aims to summarize our current understanding of the various inhibitors of inappropriate gene expression in the presumptive ectoderm.
Tyrosyl-DNA Phosphodiesterase I N-Terminal Domain Modifications and Interactions Regulate Cellular Function
Authors: Brettrager Evan J. , Segura Isaac A. , van Waardenburg Robert C. A. M.
Published: November 2019

The conserved eukaryotic DNA repair enzyme Tyrosyl-DNA phosphodiesterase I (Tdp1) removes a diverse array of adducts from the end of DNA strand breaks. Tdp1 specifically catalyzes the hydrolysis of phosphodiester linked DNA-adducts. These DNA lesions range from damaged nucleotides to peptide-DNA adducts to protein-DNA covalent complexes and are products of endogenously or exogenously induced insults or simply failed reaction products. These adducts include DNA inserted ribonucleotides and non-conventional nucleotides, as well as covalent reaction intermediates of DNA topoisomerases with DNA and a Tdp1-DNA adduct in trans. This implies that Tdp1 plays a role in maintaining genome stability and cellular homeostasis. Dysregulation of Tdp1 protein levels or catalysis shifts the equilibrium to genome instability and is associated with driving human pathologies such as cancer and neurodegeneration. In this review, we highlight the function of the N-terminal domain of Tdp1. This domain is understudied, structurally unresolved, and the least conserved in amino acid sequence and length compared to the rest of the enzyme. However, over time it emerged that the N-terminal domain was post-translationally modified by, among others, phosphorylation, SUMOylation, and Ubiquitinoylation, which regulate Tdp1 protein interactions with other DNA repair associated proteins, cellular localization, and Tdp1 protein stability.
Early Diagnosis in Prader–Willi Syndrome Reduces Obesity and Associated Co-Morbidities
Authors: Kimonis Virginia E. , Tamura Roy , Gold June-Anne , Patel Nidhi , Surampalli Abhilasha , Manazir Javeria , Miller Jennifer L. , Roof Elizabeth , Dykens Elisabeth , Butler Merlin G. , Driscoll Daniel J.
Published: November 2019

Prader–Willi syndrome (PWS) is an imprinting genetic disorder characterized by lack of expression of genes on the paternal chromosome 15q11–q13 region. Growth hormone (GH) replacement positively influences stature and body composition in PWS. Our hypothesis was that early diagnosis delays onset of obesity in PWS. We studied 352 subjects with PWS, recruited from the NIH Rare Disease Clinical Research Network, to determine if age at diagnosis, ethnicity, gender, and PWS molecular class influenced the age they first become heavy, as determined by their primary care providers, and the age they first developed an increased appetite and began seeking food. The median ages that children with PWS became heavy were 10 years, 6 years and 4 years for age at diagnosis < 1 year, between 1 and 3 years, and greater than 3 years of age, respectively. The age of diagnosis and ethnicity were significant factors influencing when PWS children first became heavy (p < 0.01), however gender and the PWS molecular class had no influence. Early diagnosis delayed the onset of becoming heavy in individuals with PWS, permitting early GH and other treatment, thus reducing the risk of obesity-associated co-morbidities. Non-white individuals had an earlier onset of becoming heavy.
Mitotic Recombination and Adaptive Genomic Changes in Human Pathogenic Fungi
Authors: Gusa Asiya , Jinks-Robertson Sue
Published: November 2019

Genome rearrangements and ploidy alterations are important for adaptive change in the pathogenic fungal species Candida and Cryptococcus, which propagate primarily through clonal, asexual reproduction. These changes can occur during mitotic growth and lead to enhanced virulence, drug resistance, and persistence in chronic infections. Examples of microevolution during the course of infection were described in both human infections and mouse models. Recent discoveries defining the role of sexual, parasexual, and unisexual cycles in the evolution of these pathogenic fungi further expanded our understanding of the diversity found in and between species. During mitotic growth, damage to DNA in the form of double-strand breaks (DSBs) is repaired, and genome integrity is restored by the homologous recombination and non-homologous end-joining pathways. In addition to faithful repair, these pathways can introduce minor sequence alterations at the break site or lead to more extensive genetic alterations that include loss of heterozygosity, inversions, duplications, deletions, and translocations. In particular, the prevalence of repetitive sequences in fungal genomes provides opportunities for structural rearrangements to be generated by non-allelic (ectopic) recombination. In this review, we describe DSB repair mechanisms and the types of resulting genome alterations that were documented in the model yeast Saccharomyces cerevisiae. The relevance of similar recombination events to stress- and drug-related adaptations and in generating species diversity are discussed for the human fungal pathogens Candida albicans and Cryptococcus neoformans.
Entirely Off-Grid and Solar-Powered DNA Sequencing of Microbial Communities during an Ice Cap Traverse Expedition
Authors: Gowers Glen-Oliver. F. , Vince Oliver , Charles John-Henry , Klarenberg Ingeborg , Ellis Tom , Edwards Arwyn
Published: November 2019

Microbial communities in remote locations remain under-studied. This is particularly true on glaciers and icecaps, which cover approximately 11% of the Earth’s surface. The principal reason for this is the inaccessibility of most of these areas due to their extreme isolation and challenging environmental conditions. While remote research stations have significantly lowered the barrier to studying the microbial communities on icecaps, their use has led to a bias for data collection in the near vicinity of these institutions. Here, miniaturisation of a DNA sequencing lab suitable for off-grid metagenomic studies is demonstrated. Using human power alone, this lab was transported across Europe’s largest ice cap (Vatnajökull, Iceland) by ski and sledge. After 11 days of unsupported polar-style travel, a metagenomic study of a geothermal hot spring gorge was conducted on the remote northern edge of the ice cap. This tent-based metagenomic study resulted in over 24 h of Nanopore sequencing, powered by solar power alone. This study demonstrates the ability to conduct DNA sequencing in remote locations, far from civilised resources (mechanised transport, external power supply, internet connection, etc.), whilst greatly reducing the time from sample collection to data acquisition.
Snow Avalanche Impact Measurements at the Seehore Test Site in Aosta Valley (NW Italian Alps)
Authors: Maggioni Margherita , Barbero Monica , Barpi Fabrizio , Borri-Brunetto Mauro , De Biagi Valerio , Freppaz Michele , Frigo Barbara , Pallara Oronzo , Chiaia Bernardino
Published: November 2019

In full-scale snow avalanche test sites, structures such as pylons, plates, or dams have been used to measure impact forces and pressures from avalanches. Impact pressures are of extreme importance when dealing with issues such as hazard mapping and the design of buildings exposed to avalanches. In this paper, we present the force measurements recorded for five selected avalanches that occurred at the Seehore test site in Aosta Valley (NW Italian Alps). The five avalanches were small to medium-sized and cover a wide range in terms of snow characteristics and flow dynamics. Our aim was to analyze the force and pressure measurements with respect to the avalanche characteristics. We measured pressures in the range of 2 to 30 kPa. Though without exhaustive measurements of the avalanche flows, we found indications of different flow regimes. For example, we could appreciate some differences in the vertical profile of the pressures recorded for wet dense avalanches and powder ones. Being aware of the fact that more complete measurements are necessary to fully describe the avalanche flows, we think that the data of the five avalanches triggered at the Seehore test site might add some useful information to the ongoing scientific discussion on avalanche flow regimes and impact pressure.
Comparison of Aggregated N-of-1 Trials with Parallel and Crossover Randomized Controlled Trials Using Simulation Studies
Authors: Blackston J. , Chapple Andrew , McGree James , McDonald Suzanne , Nikles Jane
Published: November 2019

Background: N-of-1 trials offer an innovative approach to delivering personalized clinical care together with population-level research. While increasingly used, these methods have raised some statistical concerns in the healthcare community. Methods: We discuss concerns of selection bias, carryover effects from treatment, and trial data analysis conceptually, then rigorously evaluate concerns of effect sizes, power and sample size through simulation study. Four variance structures for patient heterogeneity and model error are considered in a series of 5000 simulated trials with 3 cycles, which compare aggregated N-of-1 trials to parallel randomized controlled trials (RCTs) and crossover trials. Results: Aggregated N-of-1 trials outperformed both traditional parallel RCT and crossover designs when these trial designs were simulated in terms of power and required sample size to obtain a given power. N-of-1 designs resulted in a higher type-I error probability than parallel RCT and cross over designs when moderate-to-strong carryover effects were not considered or in the presence of modeled selection bias. However, N-of-1 designs allowed better estimation of patient-level random effects. These results reinforce the need to account for these factors when planning N-of-1 trials. Conclusion: N-of-1 trial designs offer a rigorous method for advancing personalized medicine and healthcare with the potential to minimize costs and resources. Interventions can be tested with adequate power with far fewer patients than traditional RCT and crossover designs. Operating characteristics compare favorably to both traditional RCT and crossover designs.
Dynamic Linkages among Economic Development, Energy Consumption, Environment and Health Sustainable in EU and Non-EU Countries
Authors: Feng Yongqi , Yu Xinye , Chiu Yung-ho , Chang Tzu-Han
Published: November 2019

There is a close and important relationship between environmental pollution and public health, and environmental pollution has an important impact on the public health. This study employed the two-stage meta-frontier dynamic network data envelopment analysis (TMDN-DEA) model to explore the environment pollution effects from energy consumption on the mortality of children and adult, tuberculosis rate, survival rate and health expenditure efficiencies in 28 EU countries and 53 non-EU countries from 2010 to 2014. We calculated the overall efficiency scores and the technology gap ratios of each EU and non-EU countries and the efficiencies of input and output variables in the production and health stage. The average overall efficiencies each year in EU countries are higher than in the non-EU countries. But EU countries have higher energy efficiency than non-EU countries, and non-EU countries have higher health efficiency than EU countries. The health expenditure efficiencies in the EU countries are obviously lower than those in non-EU countries. The renewable energy efficiencies are obviously higher than the non-renewable energy efficiencies; PM2.5 efficiencies are obviously higher than the CO2 efficiencies and the children’s mortality rate efficiencies are higher than the adult’s mortality rate efficiencies for EU countries and non-EU countries. The government management in the EU and non-EU countries should be strengthened to reduce the air pollutant and carbon dioxide emissions and raise energy transformation to the clean energy in renewable energy and improve health efficiencies in medical and health care field.
Application of Satellite Rainfall Products for Flood Inundation Modelling in Kelantan River Basin, Malaysia
Authors: Tam Tze Huey , Abd Rahman Muhammad Zulkarnain , Harun Sobri , Hanapi Muhammad Nassir , Kaoje Ismaila Usman
Published: November 2019

The advent of satellite rainfall products can provide a solution to the scarcity of observed rainfall data. The present study aims to evaluate the performance of high spatial-temporal resolution satellite rainfall products (SRPs) and rain gauge data in hydrological modelling and flood inundation mapping. Four SRPs, Integrated Multi-satellitE Retrievals for Global Precipitation Measurement (GPM) - Early, - Late (IMERG-E, IMERG-L), Global Satellite Mapping of Precipitation-Near Real Time (GSMaP-NRT), and Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks- Cloud Classification System (PERSIANN-CCS) and rain gauge data were used as the primary input to a hydrological model, Rainfall-Runoff-Inundation (RRI) and the simulated flood level and runoff were compared with the observed data using statistical metrics. GSMaP showed the best performance in simulating hourly runoff with the lowest relative bias (RB) and the highest Nash-Sutcliffe efficiency (NSE) of 4.9% and 0.79, respectively. Meanwhile, the rain gauge data was able to produce runoff with −12.2% and 0.71 for RB and NSE, respectively. The other three SRPs showed acceptable results in daily discharge simulation (NSE value between 0.42 and 0.49, and RB value between −23.3% and −31.2%). The generated flood map also agreed with the published information. In general, the SRPs, particularly the GSMaP, showed their ability to support rapid flood forecasting required for early warning of floods.
Knowledge towards Cervical and Breast Cancers among Industrial Workers: Results from a Multisite Study in Northern Vietnam
Authors: Tran Bach Xuan , Vo Tracy , Dang Anh Kim , Nguyen Quang Nhat , Nguyen Cuong Tat , Hoang Chi Linh , Do Khanh Nam , Latkin Carl A. , Ho Cyrus S. H. , Ho Roger C. M.
Published: November 2019

Breast and cervical cancer cases are rising among service and industrial women workers in Vietnam. We conducted a cross-sectional study among 287 workers in three factories in Hanoi and Bac Ninh from July to September 2018 to describe the knowledge of these cancers among industrial workers in Northern Vietnam using a structured questionnaire. Factors associated with knowledge of breast and cervical cancer were identified using generalized linear models (GLM). In our study, approximately one-third of participants believed breast cancer was caused by the lack of breastfeeding, exposure to pollution, and chemicals. Less than 50% knew about sexually transmitted infections that can cause cervical cancer or were aware of a vaccine for cervical cancer. Having one sexual partner within the last year was positively associated with having a higher score of knowledge for both diseases. Receiving a medical checkup within the last 12 months and seeking health information via the internet were related to greater breast cancer knowledge. Targeted education campaigns are needed to ensure proper knowledge and improve awareness of breast cancer and cervical cancer among industrial workers.
Dissipation Theory-Based Ecological Protection and Restoration Scheme Construction for Reclamation Projects and Adjacent Marine Ecosystems
Authors: Huang Faming , Lin Yanhong , Zhao Rongrong , Qin Xuan , Chen Qiuming , Lin Jie
Published: November 2019

According to the 2017 results of the Special Inspector of Sea Reclamation, a substantial number of idle reclamation zones existed in 11 provinces (cities) along the coast of China. To improve the protection level of coastal wetlands and strictly control reclamation activities, it is necessary to carry out ecological restoration of reclamation projects and adjacent marine ecosystems. The characteristics of Guanghai Bay and its reclamation project are typical in China’s coastal areas, making it an optimal representative site for this study. The dissipative structure and entropy theory was used to analyze ecological problems and environmental threats. The analytic hierarchy process was applied to determine the order of the negative entropy flow importance. The entropy increase and decrease mechanism was used to determine an ecological protection and restoration scheme for the reclamation, including the reclamation of wetland resource restoration, shoreline landscape restoration, environmental pollution control, and marine biological resource restoration. Finally, based on system logic, a typical ecological restoration system was constructed east of Guanghai Bay, with the mangrove wetland area as the model in the north and the artificial sandbeach recreation area as the focus in the south.
The Relative Risk of Homelessness among Persons with a Disability: New Methods and Policy Insights
Authors: Beer Andrew , Baker Emma , Lester Laurence , Daniel Lyrian
Published: November 2019

This paper reports on the first phase of an ambitious program of research that seeks to both understand the risk of homelessness amongst persons with a disability in Australia and shed light on the impact of a significant policy reform—the introduction of the National Disability Insurance Scheme (NDIS)—in changing the level of homelessness risk. This first paper, reports on the level of homelessness risk for persons with a disability prior to the introduction of the NDIS, with a subsequent paper providing updated data and analysis for the period post the implementation of the NDIS. In one sense, this paper provides the ‘base’ condition prior to the introduction of the NDIS but also serves a far broader role in advancing our understanding of how disability and chronic ill-health affects the risk of homelessness. Our research finds that in the period prior to the introduction of the NDIS, a large proportion of people with disabilities were at risk of homelessness, but those whose disabilities affected their schooling or employment were at the greatest risk.
Rotavirus Seasonality: An Application of Singular Spectrum Analysis and Polyharmonic Modeling
Authors: Alsova Olga K. , Loktev Valery B. , Naumova Elena N.
Published: November 2019

The dynamics of many viral infections, including rotaviral infections (RIs), are known to have a complex non-linear, non-stationary structure with strong seasonality indicative of virus and host sensitivity to environmental conditions. However, analytical tools suitable for the identification of seasonal peaks are limited. We introduced a two-step procedure to determine seasonal patterns in RI and examined the relationship between daily rates of rotaviral infection and ambient temperature in cold climates in three Russian cities: Chelyabinsk, Yekaterinburg, and Barnaul from 2005 to 2011. We described the structure of temporal variations using a new class of singular spectral analysis (SSA) models based on the “Caterpillar” algorithm. We then fitted Poisson polyharmonic regression (PPHR) models and examined the relationship between daily RI rates and ambient temperature. In SSA models, RI rates reached their seasonal peaks around 24 February, 5 March, and 12 March (i.e., the 55.17 ± 3.21, 64.17 ± 5.12, and 71.11 ± 7.48 day of the year) in Chelyabinsk, Yekaterinburg, and Barnaul, respectively. Yet, in all three cities, the minimum temperature was observed, on average, to be on 15 January, which translates to a lag between the peak in disease incidence and time of temperature minimum of 38–40 days for Chelyabinsk, 45–49 days in Yekaterinburg, and 56–59 days in Barnaul. The proposed approach takes advantage of an accurate description of the time series data offered by the SSA-model coupled with a straightforward interpretation of the PPHR model. By better tailoring analytical methodology to estimate seasonal features and understand the relationships between infection and environmental conditions, regional and global disease forecasting can be further improved.
An Exploration of Complementary Feeding Practices, Information Needs and Sources
Authors: Garcia Ada L. , Looby Sarah , McLean-Guthrie Kimberley , Parrett Alison
Published: November 2019

Following complementary feeding (CF) guidelines might be challenging for mothers lacking time, resources and/or information. We aimed to explore CF practices, information needs and channels used to obtain information in parents living in areas of socioeconomic deprivation. Sixty-four parents of infants aged 4–12 months completed a short questionnaire and 21 were interviewed. Mean (SD) weaning age was 5 ± 2.5 months, foods given >7 times/week included commercial baby foods (33%) and fruits (39%) while 86% gave formula daily. The main sources of CF information were friends and family (91%), the internet (89%) and health visitors (77%). Online forums (20%), e.g., Facebook and Netmums, were used to talk to other parents because they felt that “not enough” information was given to them by health professionals. Parents felt access to practical information was limited and identified weaning classes or online video tutorials could help meet their needs. Themes identified in qualitative findings were (1) weaning practices (i.e., concerns with child’s eating; and (2) information sources and needs (i.e., trust in the National Health Service (NHS) as a reliable source, need for practical advice). In conclusion, parents are accessing information from a number of non-evidence-based sources and they express the need for more practical advice.
A Novel Method in Surface Water Quality Assessment Based on Improved Variable Fuzzy Set Pair Analysis
Authors: Liu Yucheng , Wang Chuansheng , Chun Yutong , Yang Luxin , Chen Wei , Ding Jack
Published: November 2019

In the case of surface water pollution, it is important and necessary to accurately assess the level of contaminated water and ensure the safety of drinking water for people in disaster areas during floods. However, for the assessment of the strict requirements of drinking water, traditional assessment methods still have some limitations, such as low precision and rationality. In order to overcome these limitations, in the light of the theory of set pair analysis and variable fuzzy set, we propose an improved variable fuzzy set pair analysis method (IVFSPA), which combines the analysis framework of variable fuzzy set and set pair analysis, and has made some improvements to the fusion architecture. Firstly, we present a novel game theory comprehensive weighting method, in which the objective entropy method and the subjective analytic hierarchy process(AHP) method employed to obtain the reasonable weight. Then, based on the Nemerow index method, we improve the arithmetic form of “ P i ” (Equation P) to replace the fuzzy comprehensive evaluation method. Furthermore, we design a double judgment mode of combining the principle of maximum membership degree with the positive and negative relationship between the standard value and the measured value, which can accurately judge the evaluation level of surface water quality. Finally, to validate and verify the effectiveness of the proposed method, experiments was conducted at the representative river collection sections of Nanking, China, employing water quality data of 14 sampling sections in their rivers in Nanking during the 2017 flood. In terms of performance metcrics of precision and rationality, based on the values of “TP”, “NH3-N”, “Pb”, “AS” and “KMnO4” of “Ch-lh section/Chuhe gate” are 0.415, 3.77, 0.07, 0.23 and 7.12, respectively, the level of Ch-lh section/Chuhe gate is that the IVFSPA is Class V and the rest are class IV. Results of experiments show that our IVFSPA method can achieve a good performance, compared with other traditional methods.
Factors Associated with Sleep Disorders among Methadone-Maintained Drug Users in Vietnam
Authors: Le Tuan Anh , Dang Anh Duc , Tran An Ha Thi , Nguyen Long Hoang , Nguyen Trang Huyen Thi , Phan Hai Thanh , Latkin Carl A. , Tran Bach Xuan , Ho Cyrus S.H. , Ho Roger C.M.
Published: November 2019

Sleep quality among heroin-dependent patients receiving methadone maintenance treatment (MMT) is not fully investigated in Vietnam. This study explored the prevalence of poor sleep quality in methadone-maintained patients and associated factors. This cross-sectional included 395 MMT patients at three clinics in Nam Dinh province, Vietnam. The Pittsburgh Sleep Quality Index (PSQI) was employed to measure patients’ sleep quality. Sociodemographic, clinical, behavioral, psychological, and social support characteristics were collected. Multivariate Logistic and Generalized Linear Regression models were applied to identify associated factors. Among 395 patients, 26.6% had poor sleep quality according to the PSQI scale. People having jobs were less likely to have poor sleep quality and lower PSQI scores compared to unemployed patients. Those having spouses had lower PSQI scores than single patients. High depression, anxiety, and stress scores were associated with poor sleep quality and high PSQI scores. A longer duration of MMT increased the likelihood of experiencing poor sleep quality. Patients smoking tobacco daily or concurrently using drugs had lower PSQI scores than those that did not. This study highlights a moderate prevalence of poor sleep quality among Vietnamese MMT patients. Regular evaluation, appropriate psychological management, and social support, as well as the provision of employment opportunities, potentially improve the sleep quality of methadone-maintained patients.
Effects of a School Based Intervention on Children’s Physical Activity and Healthy Eating: A Mixed-Methods Study
Authors: Khan Matluba , Bell Ruth
Published: November 2019

Combined diet and physical activity school-based interventions (rather than only diet or physical activity interventions) are more likely to help prevent children from becoming overweight in the long term. However, such interventions are less prevalent, and therefore, this pilot study aimed to assess the feasibility of a gardening intervention coupled with awareness about plant-based meals among 9−10 year old children in a London primary school. We recruited 60 children from two Year 5 classes, one class participated as an intervention group, and results were compared against another class who acted as the control group. Children’s physical activity (PA) was measured using GENEActiv wrist-worn accelerometers. Their fruit and vegetable intake and attitudes to and preferences in eating fruits and vegetables were measured using a self-report questionnaire. Furthermore, three focus groups were held with children in the intervention group to understand the reasons behind any change as a result of the intervention. Results are inconclusive; however, they indicate some impact on reduction of sedentary behaviour, increase of moderate to vigorous PA, knowledge of nutrition and some level of acceptance in trying new vegetables. School-based interventions involving gardening show some promise to increase children’s PA and improve their attitudes to eating fruits and vegetables.
Survey Results of a Pilot Sleep Study Near Atlanta International Airport
Authors: Rocha Sarah , Smith Michael G. , Witte Maryam , Basner Mathias
Published: November 2019

Aircraft noise can disturb the sleep of residents living near airports. To investigate potential effects of aircraft noise on sleep, recruitment surveys for a pilot field study were mailed to households around Atlanta International Airport. Survey items included questions about sleep quality, sleep disturbance by noise, noise annoyance, coping behaviors, and health. Of 3159 deliverable surveys, 319 were returned (10.1%). Calculated outdoor nighttime aircraft noise (Lnight) was significantly associated with lower sleep quality (poor or fair; odds ratio (OR) = 1.04/decibel (dB); p < 0.05), trouble falling asleep within 30 min ≥1/week (OR = 1.06/dB; p < 0.01), and trouble sleeping due to awakenings ≥1/week (OR = 1.04/dB; p < 0.05). Lnight was also associated with increased prevalence of being highly sleep disturbed (OR = 1.15/dB; p < 0.0001) and highly annoyed (OR = 1.17/dB; p < 0.0001) by aircraft noise. Furthermore Lnight was associated with several coping behaviors. Residents were more likely to report often or always closing their windows (OR = 1.05/dB; p < 0.01), consuming alcohol (OR = 1.10/dB; p < 0.05), using television (OR = 1.05/dB; p < 0.05) and using music (OR = 1.07/dB; p < 0.05) as sleep aids. There was no significant relationship between Lnight and self-reported general health or likelihood of self-reported diagnosis of sleep disorders, heart disease, hypertension or diabetes. Evidence of self-reported adverse effects of aircraft noise on sleep found in this pilot study warrant further investigation in larger, more representative subject cohorts.
Traditional Korean Medicine-Based Forest Therapy Programs Providing Electrophysiological Benefits for Elderly Individuals
Authors: Yi Jiyune , Ku Boncho , Kim Seul Gee , Khil Taegyu , Lim Youngsuwn , Shin Minja , Jeon Sookja , Kim Jingun , Kang Byunghoon , Shin Jongyeon , Kim Kahye , Jeong Ah Young , Park Jeong Hwan , Choi Jungmi , Cha Wonseok , Shin Changseob , Shin Wonsop , Kim Jaeuk U.
Published: November 2019

We aimed to develop forest therapy programs (FTPs) to prevent dementia and related health problems in the elderly population, with the assumption that health benefits are FTP-type specific and depend on the participant’s psychophysiological traits. For this purpose, we developed two distinct FTPs, namely, a guided-breathing meditation program (BP) and a walking program (WP); we adopted the approach of Sasang constitutional (SC) medicine, which categorizes individuals into one of three SC types (SC1, SC2, or SC3) for medical care. The FTPs ran 11 sessions over 11 weeks. We recruited 29/31/28 participants who were 65 years of age or older for the BP/WP/control groups, respectively; obtained electrophysiological measurements via electroencephalogram (EEG), heart rate variability (HRV), and bioimpedance; and analyzed the intervention effects with analysis of covariance. Compared with the control, the BP and WP resulted in benefits for neural activity and parasympathetic nervous activity (PNA), respectively, and both FTPs yielded distinct beneficial effects on bioimpedance. Constitution-specific effects were also present. The SC1- and SC2-type participants gained positive effects in neural activity from the BP and WP, respectively. The SC3-type participants showed improvements in PNA from the WP. In conclusion, for older individuals, both programs conferred health benefits that would help prevent dementia, and the benefits were program-specific and constitution-specific.