bluuebunny/crossref_metadata_2025 huggingface dataset:
Teaching business as business
Authors: Lusoli Alberto
Published: January 2020

Purpose

This paper aims to explore the early days of business education with the aim of understanding how the Harvard Business School (HBS) contributed to the constitution of “management” as a science-based profession. The research focuses on HBS signature pedagogy, the case method and its role in the institutionalization of managerial knowledge.

Design/methodology/approach

The research is based on a qualitative content analysis of HBS Annals published between 1908 and 1930. Through a manual coding of the Annals, the paper traces the diffusion of the case method in the curriculum and connects it with the institutional transformations that took place between 1908 and 1930.

Findings

The data show how HBS curriculum transitioned from lectures to case teaching in the aftermath of First World War. This pedagogy allowed HBS to demonstrate the possibility of systematically investigate management problems and to deliver business education at scale. The discussion argues that the case method, acting as a boundary object between business praxis and management theories, constituted management as a science-based profession.

Originality/value

Recent debates have emerged about case method’s ability to critically question socio-economic structures within which business is conducted. This paper contributes to the debate arguing that the historical and institutional factors leading to the affirmation of this pedagogical approach had a substantive role in the type of knowledge produced through its application. The findings challenge the idea that the affirmation of the case method is attributable to its epistemological primacy in investigating business problems.
The agency dilemma in anti-money laundering regulation
Authors: Naheem Mohammed Ahmad
Published: January 2020

Purpose

The purpose of this paper is to provide a comprehensive theoretical framework that can be applied to the application of anti-money laundering (AML) regulation within the banking sector.

Design/methodology/approach

The paper is linked to a PhD study to be published in Winter 2015/Spring 2016 that looks at trade-based money laundering and risk assessment using an agent–principal relationship to explain the underlying relationships affected by regulation in a ML context.

Findings

The paper finds that imposing regulation and assuming that the banking sector is simply an arm of law enforcement is not an effective approach and could actually contribute toward developing ML schemes that are too complex to be easily detected.

Practical implications

The paper has implications for the banking, regulatory and law enforcement areas involved in ML and its detection.

Originality/value

The paper offers originality in providing a comprehensive multi-agency framework that is cognisant of all factors affected by AML regulation. It extends beyond existing work that has offered agency insights into various sectors of AML and ML partners.
Global knowledge management of suspicious transaction reporting system in Japan
Authors: Yasaka Noriaki
Published: January 2020

Purpose

The purpose of this study is to make clear the structure of suspicious transaction reporting system in Japan from perspective of knowledge management. Because of the institutionalization of law, suspicious transaction reports in Japan have increased, but most of these reports are from financial institutions. Moreover, it cannot be said that the effect is used completely. It is important to increase the accuracy of the data mining method and incorporate the concept of knowledge management. Furthermore, it is desirable to use suspicious transactions from a global “knowledge management” perspective.

Design/methodology/approach

Based on the case of the Japanese transaction reporting system, Global knowledge management of suspicious transaction reporting system would be considered.

Findings

It is effective for money laundering countermeasures to deepen knowledge management of data → information → knowledge → wisdom. This brings the refinement of typology at the transaction unit to the upper level. This repetition has become more advanced and generalized knowledge. Knowledge transferred from national stage to international stage is organized as further information → knowledge → wisdom by collecting data based on that knowledge. By repeating this systematization in the process of knowledge management, global prevention measures against money laundering would be disseminated. As the result of these efforts, effective counter measures against money laundering could be sharper than before.

Originality/value

This is the first study about the analysis of suspicious transaction reporting system in Japan linked to the global knowledge management.
Detecting money laundering transactions with machine learning
Authors: Jullum Martin , Løland Anders , Huseby Ragnar Bang , Ånonsen Geir , Lorentzen Johannes
Published: January 2020

Purpose

The purpose of this paper is to develop, describe and validate a machine learning model for prioritising which financial transactions should be manually investigated for potential money laundering. The model is applied to a large data set from Norway’s largest bank, DNB.

Design/methodology/approach

A supervised machine learning model is trained by using three types of historic data: “normal” legal transactions; those flagged as suspicious by the bank’s internal alert system; and potential money laundering cases reported to the authorities. The model is trained to predict the probability that a new transaction should be reported, using information such as background information about the sender/receiver, their earlier behaviour and their transaction history.

Findings

The paper demonstrates that the common approach of not using non-reported alerts (i.e. transactions that are investigated but not reported) in the training of the model can lead to sub-optimal results. The same applies to the use of normal (un-investigated) transactions. Our developed method outperforms the bank’s current approach in terms of a fair measure of performance.

Originality/value

This research study is one of very few published anti-money laundering (AML) models for suspicious transactions that have been applied to a realistically sized data set. The paper also presents a new performance measure specifically tailored to compare the proposed method to the bank’s existing AML system.
A Liberal Marxism? Mutual Care, Global Humanity and Minimum Utopia
Authors: Pitts Frederick Harry
Published: January 2020

This article surveys The Norman Geras Reader: ‘What’s There is There’, a recent collection of the work of Norman Geras, edited by Eve Garrard and Ben Cohen. The article explores the relevance of Geras’s attempted reconciliation between liberalism and Marxism to some of the key issues confronting the contemporary left: foreign policy and the failures of humanitarian intervention and non‐intervention; internationalism and the necessity for solidarity across borders in an age of nationalist populism; left approaches to totalitarianism and antisemitism; the possibilities and limitations of alternatives to and critiques of liberal capitalism; and the reinvigoration of utopian imaginaries and the futures they promise. It suggests that important lessons for the left can be unpicked from the contested legacy of the ‘decent leftism’ Geras represents, where reapplied in the wake of new political and diplomatic challenges.
The materials of indigeneity: slate and cement in a Taiwanese indigenous (Paiwan) mountain settlement
Authors: Gowlland Geoffrey
Published: January 2020

This article considers the implications of the adoption of cement and concrete as building materials in a mountain settlement of the Paiwan, one of the indigenous peoples of Taiwan. The presence of these materials in an indigenous settlement might at first glance be interpreted as a sign of cultural assimilation. This article suggests rather that, by paying close attention to the ways in which locals engage with the changing properties of imported materials in work and daily life, a more complex understanding can be gained of the negotiated relations of power between indigenous people, the state, and market forces, as well as the role of materials in these negotiations. For the Paiwan, creative engagements with foreign materials and their changing properties have, at various moments, represented ways of adapting to the pressures to assimilate, or, conversely, resulted in expressions of cultural resilience.
Antibiotic prescribing in patients who presented to the emergency department with dog bites: A descriptive review of current practice
Authors: Baxter Megan , Denny Kerina J , Keijzers Gerben
Published: January 2020

Objective

To describe the current practice of prophylactic antibiotic prescribing for patients presenting to the ED with a dog bite, and compare management against existing guidelines.

Methods

We performed a descriptive retrospective study on all consecutive patients who presented to one tertiary teaching hospital and one regional district hospital in Southeast Queensland between 1 July 2017 and 30 June 2018 with a presentation of a dog bite. Data on demographics and management were collected from the electronic medical record using a standardised data collection tool. Risk factors supporting prophylaxis were taken from the electronic Therapeutic Guidelines.

Results

Of the 336 patients included for analysis, 299 received antibiotics, of which 23 were for established infection. A total of 276 (82% of overall cohort) received a prescription for prophylactic antibiotics, either in hospital (ED or admitting ward) and/or on discharge. Of the 178 patients who received prophylactic antibiotics in hospital, 91 (51.1%) received intravenous antibiotics. Of the patients who presented to ED without a previously established infection 271 (86.6%) received prophylactic antibiotics on discharge. Over one quarter (27.5%) of patients who were given prophylactic antibiotics did not meet any high‐risk factors as outlined in guidelines.

Conclusion

Prophylactic antibiotics are extensively used for patients with dog bites. There is scope to rationalise antibiotic use and route of antibiotic administration in patients with dog bites.
A Floating Astronomical Time Scale for the Early Late Cretaceous Continental Strata in the Songliao Basin, Northeastern China
Authors: MA Xiaojuan , WU Huaichun , FANG Qiang , SHI Meinan , ZHANG Shihong , YANG Tianshui , LI Haiyan , WANG Chengshan
Published: January 2020

A continuous terrestrial succession was recovered from the Songke‐2 (SK‐2) borehole in the Songliao Basin, Northeastern China. This borehole provides a unique material for further research on the continental paleoclimate during Cretaceous greenhouse period, following a series of achievements of the Songke‐1 (SK‐1) core. In this study, thorium (Th) logging data were chosen as a paleoclimate proxy to conduct a detailed cyclostratigraphic analysis. The Th series varies quasi‐periodically; power spectra and evolutionary fast Fourier transformation (FFT) analysis reveal significant cycles in the Quantou (K2q), Qingshankou (K2qn), Yaojia (Ky and Nenjiang (K2n) formations. The ratio of cycle wavelengths in these stratigraphic units is approximately 20:5:2:1, corresponding to long orbital eccentricity (405 kyr), short orbital eccentricity (100 kyr), obliquity (37 kyr), and precession cycles (22.5 kyr and 18.4 kyr). The durations of the K2n, K2y, K2qn and K2q are estimated as 6.97, 1.83, 5.30 and 4.52 Myr, respectively, based on the constructed ∼18.62 Myr “floating” astronomical time scale (ATS). Comparison of the durations between the SK‐1 s and SK‐2 boreholes exhibits a slight difference of 0.06 Myr and 0.459 Myr for K2qn and K2y. Nevertheless, our ATS of K2n supports the chronostratigraphic frame constructed by the CA‐ID‐TIMS data of the SK‐1s borehole. This new “floating” ATS provides precise numerical ages for stratigraphic boundaries, biozones and geological events in the Songliao Basin, and can serve as a basis for correlation of strata and events between marine and terrestrial systems.
Destruction of the Northern Margin of the North China Craton in Mid‐Late Triassic: Evidence from Asthenosphere‐derived Mafic Enclaves in the Jiefangyingzi Granitic Pluton from the Chifeng Area, Southern Inner Mongolia
Authors: LIU Jianfeng , LI Jinyi , CHI Xiaoguo , ZHENG Peixi , HU Zhaochu , ZHANG Xiaowei
Published: January 2020

The petrology, geochronology and geochemistry of the mafic enclaves in the Mid‐Late Triassic Jiefangyingzi pluton from Chifeng area, southern Inner Mongolia, in China are studied to reveal their petrogenetic relationship with the host pluton. Furthermore, the coeval magmatic assemblage and its petrogenesis on the northern margin of the North China craton (NCC) are studied synthetically to elucidate their tectonic setting and the implications for the destruction of the NCC. Zircon U‐Pb dating reveals that the mafic enclaves formed at 230.4 ± 2.2 Ma, which is similar to the age of the host pluton. The most basic mafic enclaves belong to weak alkaline rocks, and they display rare earth element (REE) and trace element normalized patterns and trace element compositions similar to those of ocean island basalt (OIB). In addition, they have positive ∊Nd(t) values (+3.84 to +4.94) similar to those of the Cenozoic basalts on the northern margin of the NCC. All of these geochemical characteristics suggest that the basic mafic rocks originated from the asthenosphere. Petrological and geochemical studies suggest that the Jiefangyingzi pluton and the intermediate mafic enclaves were formed by the mixing of the asthenosphere‐derived and crust‐derived magmas in different degrees. The Mid‐Late Triassic magmatic rocks on the northern margin of the NCC could be classified into three assemblages according to their geochemical compositions: alkaline series, weak alkaline–sub‐alkaline series and sub‐alkaline series rocks. Petrogenetic analyses suggest that the upwelling of the asthenosphere played an important role in the formation of these Mid‐Late Triassic magmatic rocks. Basing on an analysis of regional geological data, we suggest that the northern margin of the NCC underwent destruction due to the upwelling of the asthenosphere during the Mid‐Late Triassic, which was induced by the delamination of the root of the collisional orogeny between Sino‐Korean and Siberian paleoplates in Late Permian.
Development of Sulfuric Acid Speleogenetic Deposits within Cavernous Middle Eocene Beds: Inference on Hydrocarbon Gas Seepages, Giza Pyramids Plateau, Egypt
Authors: BADAWY Hanan S.
Published: January 2020

Development of sulfuric acid speleogenetic mineral deposits within cavernous middle Eocene beds of the Pyramids plateau is linked to hydrocarbon gas seepages. The work carried out field observations, binocular, polarizing, scanning and transmitted electron microscopy investigation, X‐ray diffraction and X‐ray fluorescence analyses. The morphological and petrographic features and chemical composition of the studied mineral deposits reveal a hypogene sulfuric acid speleogenesis. A model comprised of the following stages can be used to explain the presence of these features. (1) Ascent of hydrogen sulfide (H2S) gas associated with hydrocarbon seepages from the Cretaceous reservoirs under reducing conditions followed by oxidation to sulfuric acid (H2SO4), (2) descent of carbonic acid (H2CO3)‐rich solution generated from surface sources, (3) reaction of H2SO4 and H2CO3 with the calcareous and argillaceous host rocks, (4) formation of H2SO4 speleogenetic by‐products represented by natroalunite, aluminium‐phosphate‐sulfate, hydrated halloysite and Fe/Mn oxides within the replacive gypsum, and (5) subsequent stresses due to the formation of nearby stratiform cavities gave rise to the development of fractures/veinlets filled with displacive fibrous satinspar gypsum. The study sets the paleokarst features of the Giza Pyramids plateau within a hypogene sulfuric acid karst system developed by the action of groundwater containing H2S, H2SO4 and H2CO3.
Circular RNA 0007255 regulates the progression of breast cancer through miR‐335‐5p/SIX2 axis
Authors: Jia Qianxin , Ye Lanlan , Xu Shangwen , Xiao Hui , Xu Siding , Shi Zhaoyin , Li Jinsheng , Chen Ziqian
Published: January 2020

Background

Breast cancer (BC) is a common cancer in women worldwide. Emerging evidence has indicated that circular RNA hsa‐circ_0007255 (circ_0007255) is a prognostic mediator in BC progression. However, the functional role of circ_0007255 needs to be determined.

Methods

The expression of circ_0007255, microRNA (miR)‐335‐5p, and SIX Homeobox 2 (SIX2) was evaluated using quantitative real‐time polymerase chain reaction (qRT‐PCR) or western blot assay. Actinomycin D and RNase R treatment was performed to analyze the stability of circ_0007255. Additionally, Seahorse extracellular flux, colony formation and transwell analyses were carried out to detect oxygen consumption ratio (OCR), colony formation and cell mobility, respectively. The interaction between miR‐335‐5p and circ_0007255 or SIX2 was confirmed via dual‐luciferase reporter assay. A xenograft tumor model was established to explore the role of circ_0007255 in vivo.

Results

Circ_0007255 and SIX2 were overexpressed, but miR‐335‐5p was diminished in BC tissues and cells. Circ_0007255 absence inhibited oxygen consumption, colony formation, cell migration and invasion, and these effects were particularly abrogated via miR‐335‐5p upregulation in BC cells. Moreover, SIX2 deficiency eliminated the promotion effects of miR‐335‐5p inhibitor on oxygen consumption, colony formation, and cell mobility in BC cells. Importantly, circ_0007255 inhibited tumor growth in vivo. Mechanically, circ_0007255 was a sponge of miR‐335‐5p to regulate SIX2 expression in BC progression.

Conclusion

Circ_0007255 functioned as a novel oncogene in the progression of BC by regulating miR‐335‐5p/SIX2 axis, and might be a promising biomarker for BC treatment.

Key points

Significant findings of the study: Levels of circ_0007255 and SIX2 were upregulated, but miR‐335‐5p was diminished in BC tissues and cells. Circ_0007255 was an oncogene in BC development and exerted its function via miR‐335‐5p/SIX2 axis in BC. Tumor growth was reduced by circ_0007255 absence.

What this study adds: Circ_0007255 functioned as a novel oncogene in the progression of BC by regulating miR‐335‐5p/SIX2 axis, and might be a promising biomarker for BC treatment.
Practical insight into upright breech birth from birth videos: A structured analysis
Authors: Reitter Anke , Halliday Alexandra , Walker Shawn
Published: January 2020

Background

We aimed to identify common features of upright vaginal breech births with good outcomes to refine a physiological approach to teaching breech birth.

Methods

We performed a structured analysis of 42 videos of successful upright breech births (eg, kneeling, hand/knees), facilitated by obstetricians (n = 34) and midwives (n = 8) in nine different countries. Precise timings and relevant clinical details were recorded on an Excel spreadsheet. Each video was analyzed twice by at least two members of the research team. Time‐to‐event intervals, frequencies of interventions, and descriptive statistics were calculated using SPSS.

Results

A completely spontaneous (labor mechanisms and maternal effort only) birth occurred in 14/42 (33%) cases. The median time between the birth of the fetal pelvis and the head in all births was 1:52 (IQR 1:05,2:46; min:sec). Lack of spontaneous rotation to a sacro‐anterior position by the time the fetus had emerged to the nipple line was strongly associated with fetal arm entrapment. The following maneuvers were used: shoulder press to flex the aftercoming head in midpelvis or outlet (n = 24), sweeping down arm/s (n = 12), buttock lift to assist shoulder press (n = 6), modified Mauriceau (n = 6), rotational maneuvers to release an entrapped arm (n = 6), elevate and rotate fetal head to assist engagement (n = 2), and conversion into supine maternal position (n = 2).

Conclusions

Most upright breech births occur within 3 minutes of the birth of the fetal pelvis. Upright breech birth attendants use variations of traditional maneuvers. We introduce a physiological breech algorithm as an initial timekeeping framework for teaching, research, and practice.
Red cell transfusion in outpatients with myelodysplastic syndromes: a feasibility and exploratory randomised trial
Authors: Stanworth Simon J. , Killick Sally , McQuilten Zoe K. , Karakantza Marina , Weinkove Robert , Smethurst Heather , Pankhurst Laura A. , Hodge Renate L. , Hopkins Valerie , Thomas Helen L. , Deary Alison J. , Callum Jeannie , Lin Yulia , Wood Erica M. , Buckstein Rena , Bowen David ,   the REDDS Investigators
Published: January 2020

Optimal red cell transfusion support in myelodysplastic syndromes (MDS) has not been tested and established. The aim of this study was to demonstrate feasibility of recruitment and follow‐up in an outpatient setting with an exploratory assessment of quality of life (QoL) outcomes (EORTC QLQ‐C30 and EQ‐5D‐5L). We randomised MDS patients to standardised transfusion algorithms comparing current restrictive transfusion thresholds (80 g/l, to maintain haemoglobin 85–100 g/l) with liberal thresholds (105 g/l, maintaining 110–125 g/l). The primary outcomes were measures of compliance to transfusion thresholds. Altogether 38 patients were randomised (n = 20 restrictive;n = 18 liberal) from 12 participating sites in UK, Australia and New Zealand. The compliance proportion for the intention‐to‐treat population was 86% (95% confidence interval 75–94%) and 99% (95–100%) for restrictive and liberal arms respectively. Mean pre‐transfusion haemoglobin concentrations for restrictive and liberal arms were 80 g/l (SD6) and 97 g/l (SD7). The total number of red cell units transfused on study was 82 in the restrictive and 192 in the liberal arm. In an exploratory analysis, the five main QoL domains were improved for participants in the liberal compared to restrictive arm. Our findings support the feasibility and need for a definitive trial to evaluate the effect of different red cell transfusion thresholds on patient‐centred outcomes.
Perspectives of preschool children in England with speech and language needs in the development of evidence‐based activities
Authors: Coad Jane , Harding Sam , Hambly Helen , Parker Naomi , Morgan Lydia , Marshall Julie , Roulstone Susan
Published: January 2020

Background

The existing evidence is limited in terms of perspectives of preschool children with speech and language needs and their views on activities used to support their needs. This paper discusses a stream of work from the interdisciplinary research programme known as “Child Talk,” based in England, UK. The overall purpose of this work stream was to gain the perspectives of preschool children aged 2 to 5 years and 11 months, with speech and language needs, to use in the development of an evidence‐based framework of activities.

Methods

Twenty‐four preschool children with a variety of needs from diverse backgrounds took part. An observational methodology was used to capture children's experiences. Children were filmed during a series of sessions, with innovative head‐mounted cameras worn by the children and supported by researcher field notes. Framework analysis was used to analyse the data based on the body movement, vocalization, and visual attention of the children during these sessions.

Results and Conclusions

Results included that children expressed enjoyment and engagement in the activities. The children expressed themselves and demonstrated their focus “multimodally” through combinations of body language, vocalization, and visual attention. These modalities were present across all contexts and children. It highlights the importance of encouraging participation in preschool children and consequently this innovative piece of work has national and international importance.
Cultivating Place: Urban Development and the Institutionalization of Seattle's P–Patch Community Gardens
Authors: Glennie Charlotte
Published: January 2020

How does culture influence the political and economic processes shaping cities? Socially rich but unprofitable land uses, such as community gardens, create a trade–off between maintaining local character and increasing exchange value. To understand how less profitable land uses can prevail in development conflicts, I examined documents and interviewed advocates for Seattle's P–Patch program, which has secured virtual permanence for its publicly owned garden sites. My historical analysis shows that the P–Patch advocates, endowed with significant cultural capital, appealed to notions of Seattle's place character and leveraged the city's legal–policy infrastructure to institutionalize community gardens within Seattle's urban planning framework. The gardens serve a wide constituency, including many low–income and minority residents, but as neighborhood amenities signifying urban sustainability, they also contribute to gentrification. My findings suggest that residents can leverage culture and local character to protect use value, but equity is far from inherent to this process and therefore requires deliberate consideration.
Circular RNA hsa_circ_0061825 (circ‐TFF1) contributes to breast cancer progression through targeting miR‐326/TFF1 signalling
Authors: Pan Gaofeng , Mao Anwei , Liu Jiazhe , Lu Jingfeng , Ding Junbin , Liu Weiyan
Published: January 2020

Objectives

Circular RNAs (circRNAs) are RNA transcripts that belong to non‐coding RNAs (ncRNAs), whose implication in human cancers has been recently demonstrated. However, the specific role of multiple circRNAs in breast cancer remains unidentified.

Materials and methods

Microarray analysis and bioinformatics analysis were applied to select circRNA and miRNA, respectively. The loop structure of circ‐TFF1 was confirmed using RNase R treatment, divergent primer PCR and Sanger sequencing. qRT‐PCR and Western blot were employed for gene expressions. In vitro and in vivo experiments were conducted to assess the function of circ‐TFF1 in biological processes in breast cancer cells. FISH and subcellular separation indicated circ‐TFF1 cellular distribution. Luciferase reporter and RIP assays and Pearson's correlation analysis were performed to evaluate relationships between genes.

Results

Circ‐TFF1 and TFF1 were both upregulated and positively associated with each other in breast cancer. Knockdown of circ‐TFF1 hindered breast cancer cell proliferation, migration, invasion and EMT in vitro and controlled tumour growth in vivo. Circ‐TFF1 acted as a ceRNA of TFF1 by sponging miR‐326, and its contribution to breast cancer progression was mediated by miR‐326/TFF1 axis.

Conclusions

Circ‐TFF1 is a facilitator in breast cancer relying on TFF1 by absorbing miR‐326, providing a novel promising target for BC treatment.
Early Adalimumab and Anti‐Adalimumab Antibody Levels for Prediction of Primary Nonresponse in Ankylosing Spondylitis Patients
Authors: Ding Xiaoliang , Zhu Ruifang , Wu Jian , Xue Ling , Gu Meihua , Miao Liyan
Published: January 2020

This study aimed at exploring the concentration‐effect relationship of adalimumab and early adalimumab and anti‐adalimumab antibody (AAA) levels in predicting primary nonresponse in a real‐world pilot cohort of patients with ankylosing spondylitis. Thirty‐one patients were included. The Ankylosing Spondylitis Disease Activity Score improved with increasing adalimumab trough level at week 12 and reached a major improvement with levels between 8 and 12 μg/mL. Moreover, weeks 4 and 2 adalimumab levels below 4.28 and 3.37 μg/mL were predictive of primary nonresponse (area under the curve (AUC) = 0.89, 0.88; P = 0.0003, P = 0.034, respectively). Week 4 AAA signal‐to‐noise levels were significantly higher among primary nonresponders, and the cutoff for primary nonresponse prediction was above 5.31 (AUC = 0.81; P = 0.004). Adalimumab trough levels in a range of 8–12 μg/mL are optimum to reach major improvement, and lower adalimumab with higher AAA levels at the early stage (week 4) predict primary nonresponse by supporting proactive monitoring to optimize adalimumab therapy.
Change of soil physicochemical properties, bacterial community and aggregation during desertification of grasslands in the Tibetan Plateau
Authors: Zhang Nannan , Zhong Bo , Zhao Chunzhang , Wang Entao , Wang Yanjie , Chen Dongming , Shi Fusun
Published: January 2020

The aim of this study was to evaluate the relationships among changes in the soil microstructure, soil physicochemical traits and microbial communities during desertification. Soil samples were collected from grasslands at different stages of desertification, from an area of the Tibetan Plateau. Soil aggregate fractionation and soil physicochemical properties were analysed using standard methods, and the abundance and diversity of bacteria associated with different aggregate fractions were estimated by quantitative PCR and Illumina Miseq amplicon sequencing. Sequential changes in response to desertification stages were detected in the analysed features. For instance, soil nutrients, microbial biomass C and N, water content and pH initially changed during the light desertification stage; then, the soil aggregation decreased during the intermediate stages; and finally, bacterial abundance and diversity decreased during heavy and severe desertification stages. During grassland desertification, vegetation coverage positively influenced soil aggregation by affecting soil properties (e.g., pH and SOC), whereas bacterial communities in FMi (free microaggregates) and FSC (free silt and clay) were negatively related to soil aggregation. This study reports novel information concerning the desertification process and the sequential modification of soil traits and interactions among soil physical properties (aggregate composition), soil nutrients and the soil microbial community in the grasslands of the Tibetan Plateau.

Highlights

Bacterial community was more sensitive in macroaggregates than in microaggregates.

Composition of small macroaggregates and associated bacteria changed during desertification.

Bacterial community in microaggregates, silt and clay negatively affected aggregation.

Vegetation coverage affected aggregation by changing soil traits and bacterial community.
Evolution of marine fisheries management in China from 1949 to 2019: How did China get here and where does China go next?
Authors: Su Shu , Tang Yi , Chang Bowen , Zhu Wenbin , Chen Yong
Published: January 2020

China is the world’s biggest fishing nation and a major player in the global seafood trade. Its fisheries development can decisively influence the global seafood trade, food security and marine conservation. In recent years, significant changes have taken place in China’s fisheries management priorities, policies and regulations. In this paper, we review the evolving fisheries management practices in China to delineate changes in the management policies, methods and their performances from 1949 to 2019. We determined that the following issues impede the development, implementation and enforcement of fisheries policies and regulations, namely the large size of the fishing fleet, large and poorly organized fisheries population, the “hidden” fishing capacity, uniform management approaches that sometimes fail to account for local conditions, lack of clearly defined and allocated fishing rights, limited data quality and availability, insufficient fisheries monitoring programmes, absence of a robust scientific input framework and insufficient stakeholder involvement. Combining those problems with China’s current management initiatives, we propose recommendations for China’s future fisheries reforms. We hope this paper can inform China’s marine fisheries policies and provide valuable references for further researches related to China’s sustainable fisheries management.
14‐3‐3 protein binding blocks the dimerization interface of caspase‐2
Authors: Kalabova Dana , Filandr Frantisek , Alblova Miroslava , Petrvalska Olivia , Horvath Matej , Man Petr , Obsil Tomas , Obsilova Veronika
Published: January 2020

Among all species, caspase‐2 (C2) is the most evolutionarily conserved caspase required for effective initiation of apoptosis following death stimuli. C2 is activated through dimerization and autoproteolytic cleavage and inhibited through phosphorylation at Ser139 and Ser164, within the linker between the caspase recruitment and p19 domains of the zymogen, followed by association with the adaptor protein 14‐3‐3, which maintains C2 in its immature form procaspase (proC2). However, the mechanism of 14‐3‐3‐dependent inhibition of C2 activation remains unclear. Here, we report the structural characterization of the complex between proC2 and 14‐3‐3 by hydrogen/deuterium mass spectrometry and protein crystallography to determine the molecular basis for 14‐3‐3‐mediated inhibition of C2 activation. Our data reveal that the 14‐3‐3 dimer interacts with proC2 not only through ligand‐binding grooves but also through other regions outside the central channel, thus explaining the isoform‐dependent specificity of 14‐3‐3 protein binding to proC2 and the substantially higher binding affinity of 14‐3‐3 protein to proC2 than to the doubly phosphorylated peptide. The formation of the complex between 14‐3‐3 protein and proC2 does not induce any large conformational change in proC2. Furthermore, 14‐3‐3 protein interacts with and masks both the nuclear localization sequence and the C‐terminal region of the p12 domain of proC2 through transient interactions in which both the p19 and p12 domains of proC2 are not firmly docked onto the surface of 14‐3‐3. This masked region of p12 domain is involved in C2 dimerization. Therefore, 14‐3‐3 protein likely inhibits proC2 activation by blocking its dimerization surface.

Databases

Structural data are available in the Protein Data Bank under the accession numbers 6SAD and 6S9K.
Intensity of stress singularity for the circumferential V‐shape corner front of a three‐dimensional diamond‐like defect
Authors: Ping X.C. , Zhang Y.X. , Guo Y.B. , Xiao Z.M. , Jalde J. , Chen M.C.
Published: January 2020

Three‐dimensional diamond‐like defects with circumferential V‐shape corner fronts are often contained in engineering materials. In this paper, generalized stress intensity factors are calculated for this type of defect using a modified advanced finite element method. A super corner front element model in the global coordinates is established to capture the stress singularities along the circumferential corner front. Three‐dimensional numerical series eigen‐solutions in the element have been transformed from asymptotic expressions in the local curvilinear coordinates. The element is suitable for a sharp V‐shape corner with arbitrary opening and inclination angle. Singular stress fields near various shapes of diamond‐like defects are systematically investigated. The interaction of an embedded defect with free surface or another identical defect is also investigated. The numerical results can be used as stress intensity parameters to predict fatigue strength at circumferential corner front of a diamond‐like defect.
Reducing the effect of DOAC interference in laboratory testing for factor VIII and factor IX: A comparative study using DOAC Stop and andexanet alfa to neutralize rivaroxaban effects
Authors: Favaloro Emmanuel J. , Gilmore Grace , Bonar Roslyn , Dean Elysse , Arunachalam Sandya , Mohammed Soma , Baker Ross
Published: January 2020

Introduction

Investigation of factors (F) VIII and IX is common, with testing important for diagnosis or exclusion of haemophilia A or B, associated acquired conditions and factor inhibitors. Rivaroxaban, a common direct anti‐Xa agent, causes significant interference in clotting assays, including substantial false reduction of factor levels.

Aim

To assess whether rivaroxaban‐induced interference of FVIII and FIX testing could be neutralized.

Materials and methods

An international, cross‐laboratory exercise for FVIII (n = 84) and FIX (n = 74), using four samples: (A) pool of normal plasma; (B) pool spiked with rivaroxaban (200 ng/mL); (C) rivaroxaban sample subsequently treated with ‘DOAC Stop’ and; (D) rivaroxaban sample treated with andexanet alfa (200 μg/mL). Testing performed blind to sample type.

Results

All laboratories reported normal FIX and 94% reported normal FVIII in the pool sample. Instead, 55% and 95%, respectively, reported abnormal FIX and FVIII levels for the rivaroxaban sample. DOAC Stop treatment evidenced a correction in most laboratories (100% reported normal FIX and 86% normal FVIII). Andexanet alfa provided intermediate results, with many laboratories still reporting abnormal results (59% for FVIII, 18% for FIX). We also identified reagent‐specific issues.

Conclusions

As expected, rivaroxaban caused false low values of FVIII and FIX. This might lead to increased testing to identify the cause of low factor levels and potentially lead to false identification of (mild) haemophilia A or B if unrecognized by clinicians/laboratories. DOAC Stop effectively neutralized the rivaroxaban effect, but andexanet alfa less so, with reagent‐related effects evident, and thus, false low values sometimes persisted.
CONTROLLING INFLATION WITH TIMID MONETARY–FISCAL REGIME CHANGES
Authors: Ascari Guido , Florio Anna , Gobbi Alessandro
Published: January 2020

Can monetary policy control inflation when both monetary and fiscal policies change over time? When monetary policy is active, a long‐run fiscal principle entails flexibility in fiscal policy that preserves determinacy even when deviating from passive fiscal, substantially for brief periods or timidly for prolonged periods. In order to guarantee a unique equilibrium, monetary and fiscal policies must coordinate not only within but also across regimes, and not simply on being active or passive, but also on their extent. The amplitude of deviations from the active monetary/passive fiscal benchmark determines whether a regime is Ricardian: Timid deviations do not imply wealth effects.
Accounting conservatism and the profitability of corporate insiders
Authors: Khalilov Akram , Osma Beatriz Garcia
Published: January 2020

We predict that accounting conservatism influences insiders' opportunities to speculate on good and bad news, and thus, insider trading profitability. We find that greater conditional (unconditional) conservatism is associated with lower (higher) insiders' profitability from sales. We find limited evidence that conservatism influences profitability from purchases. These findings are consistent with our hypotheses on the different informational roles of conditional and unconditional conservatism, and on the asymmetric influence of conservatism over the opportunities to speculate on good versus bad news. Our research design takes into consideration the endogenous nature of insiders' trading and conservatism. The results are robust to different measures of conservatism and a number of additional analyses.
Government contracts and US bond yield spreads: A study on costs and benefits of materialized political connections
Authors: Ngo Thanh , Susnjara Jurica
Published: January 2020

In a 1991–2013 sample of bonds issued by US public firms, we find that the cost of debt (yield spread relative to comparable Treasuries) of suppliers to government agencies is contingent on the strategic importance of the supplier's industry. The yield spreads for strategically unimportant government suppliers are higher than for firms that are not government suppliers. If government contracts serve as tangible evidence of political connections, these higher yield spreads indicate that weaker corporate governance as a cost of political connections outweighs the benefits of said connections. For the subsample of government suppliers from strategically important industries, where the benefits of implicit bailout guarantees and revenue stability outweigh the corporate governance problems, the cost of debt is lower than for firms that are not government suppliers. The higher (lower) cost of debt for strategically unimportant (strategically important) suppliers is confined to contracting with the federal government. Our findings are robust to alternative variable and sample specifications, and to endogeneity concerns.
Phylogeographical patterns and a cryptic species provide new insights into Western Indian Ocean giant clams phylogenetic relationships and colonization history
Authors: Fauvelot Cécile , Zuccon Dario , Borsa Philippe , Grulois Daphné , Magalon Hélène , Riquet Florentine , Andréfouët Serge , Berumen Michael L. , Sinclair‐Taylor Tane H. , Gélin Pauline , Behivoke Faustinato , ter Poorten Jan Johan , Strong Ellen E. , Bouchet Philippe
Published: January 2020

Aim

The unique biodiversity in the Red Sea is the result of complex ecological and evolutionary processes driven by Pleistocene climatic change. Here we investigate the species diversity, phylogenetic relationships and phylogeographical patterns of giant clams in the Western Indian Ocean (WIO) and the Red Sea to explore scenarios of marine speciation in this under‐studied region.

Location

Mozambique Channel, Madagascar, and the Mascarene Islands (WIO); the Farasan Islands (Red Sea).

Taxon

Giant clams of the genus Tridacna (Cardiidae: Tridacninae).

Methods

Giant clams were sampled as complete organisms or through underwater biopsies. Nuclear (28S) and mitochondrial (16S and COI) DNA sequences were subjected to Bayesian and maximum likelihood analyses to generate a phylogenetic hypothesis for all known species within the genus Tridacna. Bayesian inference with molecular and fossil calibration was used to infer their colonization history.

Results

From the 10 genetically distinct clades recovered from the analyses of 253 sequenced specimens, five distinct Tridacna lineages were sampled, three of which were endemics of the WIO and the Red Sea. Each lineage corresponded to a distinct species, except one grouping the two formerly known WIO endemics, T. lorenzi and T. rosewateri. This lineage clustered with two other well‐supported lineages: the Red Sea endemic T. squamosina, and a previously unrecognized lineage, restricted to the WIO, for which we resurrect the long‐forgotten name T. elongatissima Bianconi, 1856. For the two other species sampled (T. maxima and T. squamosa), contrasting phylogeographical patterns were observed.

Main conclusions

Our data confirm the validity of the species T. rosewateri, a WIO endemic genetically indistinguishable from T. lorenzi, which should be considered a junior synonym. The phylogenetic placement of the newly resurrected T. elongatissima provides insights into the probable origin of T. squamosina, which split from its sister species no later than 2 Ma, likely during Middle Pleistocene glacial periods. Two scenarios are discussed. Our results also suggest that T. maxima invaded the Red Sea more recently and that contemporary gene flow between Red Sea and WIO T. maxima is highly restricted. The deep Red Sea partition observed in T. squamosa suggests a much older divergence and raises the question of a possible cryptic lineage.
CPF impedes cell cycle re‐entry of quiescent lung cancer cells through transcriptional suppression of FACT and c‐MYC
Authors: Bi Ling , Xie Chanlu , Jiao Lijing , Jin Shenyi , Hnit Su Su Thae , Mu Yao , Wang Yilun , Wang Qian , Ge Guangbo , Wang Yaqiao , Zhao Xiaodong , Shi Xinglong , Kang Yani , De Souza Paul , Liu Tao , Zhou Jia , Xu Ling , Dong Qihan
Published: January 2020

Blockade of cell cycle re‐entry in quiescent cancer cells is a strategy to prevent cancer progression and recurrence. We investigated the action and mode of action of CPF mixture (Coptis chinensis, Pinellia ternata and Fructus trichosanthis) in impeding a proliferative switch in quiescent lung cancer cells. The results indicated that CPF impeded cell cycle re‐entry in quiescent lung cancer cells by reduction of FACT and c‐MYC mRNA and protein levels, with concomitant decrease in H3K4 tri‐methylation and RNA polymerase II occupancy at FACT and c‐MYC promoter regions. Animals implanted with quiescent cancer cells that had been exposed to CPF had reduced tumour volume/weight. Thus, CPF suppresses proliferative switching through transcriptional suppression of FACT and the c‐MYC, providing a new insight into therapeutic target and intervention method in impeding cancer recurrence.
Integrative analysis and validation of dysregulated long non‐coding RNAs in colon cancer
Authors: Zhang Qun , Bian Yinzhu , Zhu Yiping , Wan Li , Kong Linghui , Hu Jing , Yang Mi , Li Li , Liu Baorui , Qian Xiaoping
Published: January 2020

It is an increasing evidence that long non‐coding RNAs (lncRNAs) are involved in tumour initiation and progression. Here, we analysed RNA‐sequencing data from the Cancer Genome Atlas (TCGA) datasets. Totally, 1176lncRNAs, 245miRNAs and 2081mRNAs were identified to be differentially expressed (DE) in colon cancer tissues compared with normal tissues. CASC21, a novel lncRNA located in 8q24.21 locus, was significantly overexpressed in 30 colon cancer tissues compared with matched normal tissues by qRT‐PCR assay. CASC21 tended to higher expression as the increase of the tumour‐node‐metastasis (TNM) classification. Functionally, CASC21 promoted cell proliferation by regulating cell cycle and enhanced tumour metastasis by epithelial‐mesenchymal transition (EMT) in colon cancer. Mechanism study indicated that CASC21 might be involved in activating WNT/β‐catenin pathway in colon cancer. In addition, we also built a competing endogenous RNA (ceRNNA) network by bioinformatic analysis using TCGA datasets. Together, our results not only provide novel lncRNAs as potential candidates for further study but also prove that CASC21 is an oncogenic regulator through activating WNT/β‐catenin signalling in colon cancer.
Novel functions for 2‐phenylbenzimidazole‐5‐sulphonic acid: Inhibition of ovarian cancer cell responses and tumour angiogenesis
Authors: Kim Min Su , Kim Jae Hyeon , Ahn Eun‐Kyung , Cho Young‐Rak , Han Surim , Lee Choong‐Hyun , Bae Gyu‐Un , Oh Joa Sub , Kim Kyu‐Bong , Seo Dong‐Wan
Published: January 2020

In this study, we investigated the effects and molecular mechanisms of 2‐phenylbenzimidazole‐5‐sulphonic acid (PBSA), an ultraviolet B protecting agent used in sunscreen lotions and moisturizers, on ovarian cancer cell responses and tumour angiogenesis. PBSA treatment markedly blocked mitogen‐induced invasion through down‐regulation of matrix metalloproteinase (MMP) expression and activity in ovarian cancer SKOV‐3 cells. In addition, PBSA inhibited mitogen‐induced cell proliferation by suppression of cyclin‐dependent kinases (Cdks), but not cyclins, leading to pRb hypophosphorylation and G1 phase cell cycle arrest. These anti‐cancer activities of PBSA in ovarian cancer cell invasion and proliferation were mediated by the inhibition of mitogen‐activated protein kinase kinase 3/6‐p38 mitogen‐activated protein kinase (MKK3/6‐p38MAPK) activity and subsequent down‐regulation of MMP‐2, MMP‐9, Cdk4, Cdk2 and integrin β1, as evidenced by treatment with p38MAPK inhibitor SB203580. Furthermore, PBSA suppressed the expression and secretion of vascular endothelial growth factor in SKOV‐3 cells, leading to inhibition of capillary‐like tubular structures in vitro and angiogenic sprouting ex vivo. Taken together, our results demonstrate the pharmacological effects and molecular targets of PBSA on modulating ovarian cancer cell responses and tumour angiogenesis, and suggest further evaluation and development of PBSA as a promising chemotherapeutic agent for the treatment of ovarian cancer.