bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased Inflammatory Markers at AMPH-Addicts Are Related to Neurodegenerative Conditions: Alzheimer’s Disease
Authors: Alrafiah Aziza R. , Abu-Illah Mohammed M. , Magadmi Rania M. , Aqeel Aqeel , Najmi Abdulmuttaleb , Jaddoh Sattam
Published: May 2022

Amphetamine addiction is widespread worldwide despite causing severe physical and mental problems, including neurodegeneration. One of the most common neurodegenerative disorders is Alzheimer’s disease (AD). Several inflammatory markers have been linked to AD. Previous studies have also found these biomarkers in amphetamine-addicts (AMPH-add). This study thus seeks to understand how AD and AMPH-addiction are related. A case–control observational study was conducted. Seventeen AMPH-adds ranging in age from 23 to 40 were recruited from Al Amal Psychiatric Hospital. In addition, 19 healthy subjects matching their age and gender were also recruited. The Luminex technique was used to measure serum alpha 1 antichymotrypsin (ACT), pigment epithelium-derived factor (PEDF), and macrophage inflammatory protein-4 (MIP-4), after complying with ethical guidelines and obtaining informed consent. In addition, liver function enzymes were correlated to AD’s predictive biomarkers in AMPH-adds. AMPH-adds had significantly higher serum levels of ACT, PEDF, and MIP-4 when compared to healthy controls (p = 0.03, p = 0.001, and p = 0.012, respectively). Furthermore, there is a significant correlation between lower ALT levels and elevated AST to ALT ratios in AMPH-adds (r = 0.618, 0.651, and p = 0.0001). These changes in inflammatory biomarkers may be linked to the onset of AD at a young age in amphetamine-drug addicts.
Postmortem Diagnosis of Ketoacidosis by Determining Beta-Hydroxybutyrate Levels in Three Types of Body Fluids by Two Different Methods
Authors: Iliescu Diana Bulgaru , Furnica Cristina , Girlescu Nona , Chistol Raluca Ozana , Perianu Lacramioara , Diac Madalina , Timofte Andrei Daniel , Knieling Anton , Ciureanu Ioan-Adrian
Published: May 2022

Background: Postmortem assessment of endogenous ketoacidosis is primarily focused on the determination of 3-beta-hydroxybutyrate (BHB). The aim of our study was to identify the most adequate body fluid and postmortem quantification method for assessing ketoacidosis status immediately prior to death. Material and method: We performed a prospective study on 53 cases of sudden death or in-hospital death that were considered forensic cases and could present a state of ketoacidosis prior to death, the autopsies being performed at a post-mortem interval of 24–72 h. BHB analysis was performed by Multi-Functional Monitoring System XPER Technology analyzer (method A—portable analyzer) for peripheral blood, and by BHB Assay MAK041 Kit (method B) for vitreous humor (VH) and cerebrospinal fluid (CSF). Results: We identified 11 ketoacidosis cases using method A and 9 ketoacidosis cases using method B. All nine cases of ketoacidosis identified using the MAK041 kit were confirmed with the portable analyzer. For the 2 cases of ketoacidosis identified only with the portable analyzer, the values obtained by method B were at the diagnostic limit. BHB concentrations determined in VH and CSF by method B were statistically significantly correlated with each other and with peripheral blood BHB concentration. Conclusion: BHB, a marker of ketoacidosis, should be determined post-mortem whenever a metabolic imbalance is suspected irrespective of known risk factors or obvious morphological substrate to help establish the thanatogenic mechanism. BHB quantification can easily be performed using a handheld automatic analyzer and a sample of peripheral blood as BHB levels in various body fluids correlate with each other.
Empirical Analysis of Parallel Corpora and In-Depth Analysis Using LIWC
Authors: Park Chanjun , Shim Midan , Eo Sugyeong , Lee Seolhwa , Seo Jaehyung , Moon Hyeonseok , Lim Heuiseok
Published: May 2022

The machine translation system aims to translate source language into target language. Recent studies on MT systems mainly focus on neural machine translation. One factor that significantly affects the performance of NMT is the availability of high-quality parallel corpora. However, high-quality parallel corpora concerning Korean are relatively scarce compared to those associated with other high-resource languages, such as German or Italian. To address this problem, AI Hub recently released seven types of parallel corpora for Korean. In this study, we conduct an in-depth verification of the quality of corresponding parallel corpora through Linguistic Inquiry and Word Count (LIWC) and several relevant experiments. LIWC is a word-counting software program that can analyze corpora in multiple ways and extract linguistic features as a dictionary base. To the best of our knowledge, this study is the first to use LIWC to analyze parallel corpora in the field of NMT. Our findings suggest the direction of further research toward obtaining the improved quality parallel corpora through our correlation analysis in LIWC and NMT performance.
Influence of Random Modulated Power Converter on G3 Power Line Communication
Authors: Beshir Abduselam Hamid , El Sayed Waseem , Wan Lu , Grassi Flavia , Crovetti Paolo Stefano , Liu Xiaokang , Wu Xinglong , Madi Amr , Smolenski Robert , Pignari Sergio Amedeo
Published: May 2022

Power Line Communication (PLC) technologies are being used in many applications and offer the advantage of utilizing existing power cables for both power and data transmission, thus minimizing cost and complexity. Nevertheless, PLC technology requires further investigation to solve possible co-existence issues. Indeed, recent studies confirmed that alternative modulation schemes such as Random Pulse Width Modulation (RPWM), applied to switching-mode power converters to minimize conducted emissions, detrimentally interfere with the PLC system. This paper presents an experimental test campaign aimed at investigating the effects of RPWM on the G3-PLC system, with the final goal of understanding the conditions under which RPWM schemes can be considered as an effective alternative to conventional Pulse Width Modulation (PWM) in applications involving PLC systems. In details, the effects of different RPWM parameters such as switching frequency, modulation index, and Random Number Update Rate (RNUR) on the G3-PLC is investigated. In addition, different RPWM schemes such as Random Frequency Modulation (RFM) and Random Pulse Position Modulation (RPPM) are compared in terms of performance so as to highlight which RPWM is best suited to assure coexistence with PLC systems. The impact of RPWM on the communication channel is evaluated in terms of Frame Error Rate (FER), Channel Capacity, and Channel Capacity Loss metrics. Experimental results confirmed that randomly modulated converters with switching frequencies near the G3-PLC bandwidth cause more significant disturbance and possible coexistence issues than the switching frequencies out of this range. Results also show that the modulation index and the RNUR of RPWM have a direct effect on the communication channel. Moreover, a trade-off between Electromagnetic Interference (EMI) reduction and coexistence issues is observed: RFM, which is very effective for EMI reduction, is found to be very disruptive for G3-PLC, compared to alternative random modulation techniques such as RPPM.
Possible Blockchain Solutions According to a Smart City Digitalization Strategy
Authors: Lukić Ivica , Miličević Kruno , Köhler Mirko , Vinko Davor
Published: May 2022

With advances in Information and Communication Technologies (ICT) in convergence with blockchain technology, cities have been given the opportunity to improve their services, efficiently use resources, and, thus, become Smart Cities. The main properties of blockchain technology like decentralization, immutability, transparency, consensus, and robustness are qualities needed for Smart City. In this paper, we propose a digitalization strategy for the City of Osijek. Smart City digitalization strategy aims to solve problems of emerging urbanization, improve administration by reducing energy and water consumption, carbon emissions, pollution, and city waste management. To develop an information system based on blockchain technology, the administration structure and the current state of information systems are analyzed, and new solutions are presented.
Telepresence Social Robotics towards Co-Presence: A Review
Authors: Almeida Luis , Menezes Paulo , Dias Jorge
Published: May 2022

Telepresence robots are becoming popular in social interactions involving health care, elderly assistance, guidance, or office meetings. There are two types of human psychological experiences to consider in robot-mediated interactions: (1) telepresence, in which a user develops a sense of being present near the remote interlocutor, and (2) co-presence, in which a user perceives the other person as being present locally with him or her. This work presents a literature review on developments supporting robotic social interactions, contributing to improving the sense of presence and co-presence via robot mediation. This survey aims to define social presence, co-presence, identify autonomous “user-adaptive systems” for social robots, and propose a taxonomy for “co-presence” mechanisms. It presents an overview of social robotics systems, applications areas, and technical methods and provides directions for telepresence and co-presence robot design given the actual and future challenges. Finally, we suggest evaluation guidelines for these systems, having as reference face-to-face interaction.
The Benefits of Video Games on Brain Cognitive Function: A Systematic Review of Functional Magnetic Resonance Imaging Studies
Authors: Huang He , Cheng Chuanyin
Published: May 2022

Benefits of video games on cognitive function have been proved by increasing evidence. However, reasons for game-induced changes in cognitive function are still elusive. Therefore, this study conducted a systematic review of brain function activation changes in association with video games. We retrieved publications from three electronic databases (PubMed, Web of Science, and PsycInfo), with publication dates before 8 February 2021. After screening the study with fMRI data, 13 studies were included in this work, including 9 cross-sectional studies and 4 types of research. In this review, we summarized the potential benefits of video games on cognitive function and discussed the effects of different types of video games on cognitive function. In particular, we highlighted the effect of video games on attention ability and visuospatial ability and addressed the functional brain activation changes in frontal and parietal lobes and other related brain regions induced by games. Finally, we pointed out that when discussing the effect of video games on brain function, types of video games should be carefully categorized.
Multi-Source Data Fusion Method for the Truss Structure Stability Measurement of Space Telescope
Authors: Xu Tianxiao , He Xu , Zhang Xiaohui , Luo Jing , Wang Jinxin , Li Chenghao , You Chenxu , Jiang Chengqiang , Liu Yichen
Published: May 2022

It is necessary for large aperture space telescopes to achieve high measurement accuracy for mechanical reference mounting surfaces, complex deformation conditions, and difficulty in describing spatial geometric properties. In this manuscript, we propose a measurement method for evaluating the deformation of the trusses structure by fusing multiple sources of data. The multi-source data are obtained from the theodolite, laser tracker, and photogrammetry systems. The datum alignment of the laser tracker and photogrammetry coordinate systems is achieved by establishing the transition coordinate system method of the datum platform, and the alignment accuracy (3σ) is about 8.8″, 5.1″ and 4.2″. Using the laser tracker to establish the relationship between the cubic mirror coordinate system and the geometric coordinate system of the mounting part, the fast and high-precision measurement of the mounting angle of the cubic prism is realized, and the measurement accuracy reaches 2″. The data from multiple sources are fused through datum transfer and alignment to establish a cosine matrix transfer chain between the mechanical characteristic coordinate system of each component and the transition coordinate system of the datum platform, and the Eulerian rotation angle is obtained to describe the angular relationship between the components after solving. Based on the Monte Carlo mathematical error modeling analysis, the datum transfer and alignment simulations were carried out, and related experiments were conducted. The experiments show that the transfer error (3σ) of the XYZ rotation angle is less than 20.6″ after the datum transfer, the maximum error is within 3″ compared to the simulation results, and the deviation of the comparison photographic measurement data is less than 7.6″. The datum transfer method combined with photogrammetry can describe the deformation trend of large-size trusses under different load conditions more objectively and reasonably.
Resolution Characterizations of JetRIS in Mainz Using 164Dy
Authors: Münzberg Danny , Block Michael , Claessens Arno , Ferrer Rafael , Laatiaoui Mustapha , Lantis Jeremy , Nothhelfer Steven , Raeder Sebastian , Van Duppen Piet
Published: May 2022

Laser spectroscopic studies of elements in the heavy actinide and transactinide region help understand the nuclear ground state properties of these heavy systems. Pioneering experiments at GSI, Darmstadt identified the first atomic transitions in the element nobelium. For the purpose of determining nuclear properties in nobelium isotopes with higher precision, a new apparatus for high-resolution laser spectroscopy in a gas-jet called JetRIS is under development. To determine the spectral resolution and the homogeneity of the gas-jet, the laser-induced fluorescence of 164Dy atoms seeded in the jet was studied. Different hypersonic nozzles were investigated for their performance in spectral resolution and efficiency. Under optimal conditions, a spectral linewidth of about 200–250 MHz full width at half maximum and a Mach number of about 7 was achieved, which was evaluated in context of the density profile of the atoms in the gas-jet.
The Expected Value of Hosoya Index and Merrifield–Simmons Index in a Random Cyclooctylene Chain
Authors: Sun Yushuang , Geng Xianya
Published: May 2022

The Hosoya index m(G) and the Merrifield–Simmons index i(G) of a graph G are the number of matchings and the number of independent sets in G. In this paper, we establish exact formulas for the expected value of the Hosoya index and Merrifield–Simmons index of the random cyclooctylene chains, which are graphs of a chemical chain consisting of n octagons, each of which is connected to the end of the previous octagon by an edge. In addition, we obtain the expected values and the average values of the two indexes through the relevant chemical diagrams and a series of accurate formulas with respect to the set of all cyclooctylene chains with n octagons.
A Novel Method of Exploring the Uncanny Valley in Avatar Gender(Sex) and Realism Using Electromyography
Authors: Bailey Jacqueline D. , Blackmore Karen L.
Published: May 2022

Despite the variety of applications that use avatars (virtual humans), how end-users perceive avatars are not fully understood, and accurately measuring these perceptions remains a challenge. To measure end-user responses more accurately to avatars, this pilot study uses a novel methodology which aims to examine and categorize end-user facial electromyography (f-EMG) responses. These responses (n = 92) can be categorized as pleasant, unpleasant, and neutral using control images sourced from the International Affective Picture System (IAPS). This methodology can also account for variability between participant responses to avatars. The novel methodology taken here can assist in the comparisons of avatars, such as gender(sex)-based differences. To examine these gender(sex) differences, participant responses to an avatar can be categorized as either pleasant, unpleasant, neutral or a combination. Although other factors such as age may unconsciously affect the participant responses, age was not directly considered in this work. This method may allow avatar developers to better understand how end-users objectively perceive an avatar. The recommendation of this methodology is to aim for an avatar that returns a pleasant, neutral, or pleasant-neutral response, unless an unpleasant response is the intended. This methodology demonstrates a novel and useful way forward to address some of the known variability issues found in f-EMG responses, and responses to avatar realism and uncanniness that can be used to examine gender(sex) perceptions.
Joint Transcriptome and Metabolome Analysis Prevails the Biological Mechanisms Underlying the Pro-Survival Fight in In Vitro Heat-Stressed Granulosa Cells
Authors: Sammad Abdul , Luo Hanpeng , Hu Lirong , Zhao Shanjiang , Gong Jianfei , Umer Saqib , Khan Adnan , Zhu Huabin , Wang Yachun
Published: May 2022

Previous studies reported the physical, transcriptome, and metabolome changes in in vitro acute heat-stressed (38 °C versus 43 °C for 2 h) bovine granulosa cells. Granulosa cells exhibited transient proliferation senescence, oxidative stress, an increased rate of apoptosis, and a decline in steroidogenic activity. In this study, we performed a joint integration and network analysis of metabolomic and transcriptomic data to further narrow down and elucidate the role of differentially expressed genes, important metabolites, and relevant cellular and metabolic pathways in acute heat-stressed granulosa cells. Among the significant (raw p-value < 0.05) metabolic pathways where metabolites and genes converged, this study found vitamin B6 metabolism, glycine, serine and threonine metabolism, phenylalanine metabolism, arginine biosynthesis, tryptophan metabolism, arginine and proline metabolism, histidine metabolism, and glyoxylate and dicarboxylate metabolism. Important significant convergent biological pathways included ABC transporters and protein digestion and absorption, while functional signaling pathways included cAMP, mTOR, and AMPK signaling pathways together with the ovarian steroidogenesis pathway. Among the cancer pathways, the most important pathway was the central carbon metabolism in cancer. Through multiple analysis queries, progesterone, serotonin, citric acid, pyridoxal, L-lysine, succinic acid, L-glutamine, L-leucine, L-threonine, L-tyrosine, vitamin B6, choline, and CYP1B1, MAOB, VEGFA, WNT11, AOX1, ADCY2, ICAM1, PYGM, SLC2A4, SLC16A3, HSD11B2, and NOS2 appeared to be important enriched metabolites and genes, respectively. These genes, metabolites, and metabolic, cellular, and cell signaling pathways comprehensively elucidate the mechanisms underlying the intricate fight between death and survival in acute heat-stressed bovine granulosa cells and essentially help further our understanding (and will help the future quest) of research in this direction.
Dietary Phytoestrogen Intake and Cognitive Status in Southern Italian Older Adults
Authors: Giampieri Francesca , Godos Justyna , Caruso Giuseppe , Owczarek Marcin , Jurek Joanna , Castellano Sabrina , Ferri Raffaele , Caraci Filippo , Grosso Giuseppe
Published: May 2022

Background: Aging society faces significant health challenges, among which cognitive-related disorders are emerging. Diet quality has been recognized among the major contributors to the rising prevalence of cognitive disorders, with increasing evidence of the putative role of plant-based foods and their bioactive components, including polyphenols. Dietary polyphenols, including phytoestrogens, have been hypothesized to exert beneficial effects toward brain health through various molecular mechanisms. However, the evidence on the association between dietary phytoestrogen intake and cognitive function is limited. The aim of this study was to investigate the association between phytoestrogen intake and cognitive status in a cohort of older adults living in Sicily, Southern Italy. Methods: Dietary information from 883 individuals aged 50 years or older was collected through a validated food frequency questionnaire. Cognitive status was assessed through the Short Portable Mental Status Questionnaire. Results: The highest total isoflavone (including daidzein and genistein) intake was inversely associated with cognitive impairment compared to the lowest (odds ratio (OR) = 0.43, 95% confidence interval (CI): 0.20–0.92). Higher intake of total lignans and, consistently, all individual compounds (with the exception of secoisolariciresinol) were inversely associated with cognitive impairment only in the unadjusted model. Conclusions: A higher intake of phytoestrogens, especially isoflavones, was associated with a better cognitive status in a cohort of older Italian individuals living in Sicily. Taking into account the very low intake of isoflavones in Italian diets, it is noteworthy to further investigate selected populations with habitual consumption of such compounds to test whether these results may be generalized to the Italian population.
Role of Polyphenol-Derived Phenolic Acid in Mitigation of Inflammasome-Mediated Anxiety and Depression
Authors: Iban-Arias Ruth , Sebastian-Valverde Maria , Wu Henry , Lyu Weiting , Wu Qingli , Simon Jim , Pasinetti Giulio Maria
Published: May 2022

Overexposure to mental stress throughout life is a significant risk factor for the development of neuropsychiatric disorders, including depression and anxiety. The immune system can initiate a physiological response, releasing stress hormones and pro-inflammatory cytokines, in response to stressors. These effects can overcome allostatic physiological mechanisms and generate a pro-inflammatory environment with deleterious effects if occurring chronically. Previous studies in our lab have identified key anti-inflammatory properties of a bioavailable polyphenolic preparation BDPP and its ability to mitigate stress responses via the attenuation of NLRP3 inflammasome-dependent responses. Inflammasome activation is part of the first line of defense against stimuli of different natures, provides a rapid response, and, therefore, is of capital importance within the innate immunity response. malvidin-3-O-glucoside (MG), a natural anthocyanin present in high proportions in grapes, has been reported to exhibit anti-inflammatory effects, but its mechanisms remain poorly understood. This study aims to elucidate the therapeutic potential of MG on inflammasome-induced inflammation in vitro and in a mouse model of chronic unpredictable stress (CUS). Here, it is shown that MG is an anti-pyroptotic phenolic metabolite that targets NLRP3, NLRC4, and AIM2 inflammasomes, subsequently reducing caspase-1 and IL-1β protein levels in murine primary cortical microglia and the brain, as its beneficial effect to counteract anxiety and depression is also demonstrated. The present study supports the role of MG to mitigate bacterial-mediated inflammation (lipopolysaccharide or LPS) in vitro and CUS-induced behavior impairment in vivo to address stress-induced inflammasome-mediated innate response.
Brain Protective Effect of Resveratrol via Ameliorating Interleukin-1β-Induced MMP-9-Mediated Disruption of ZO-1 Arranged Integrity
Authors: Tsai Ming-Ming , Chen Jiun-Liang , Lee Tsong-Hai , Liu Hsiuming , Shanmugam Velayuthaprabhu , Hsieh Hsi-Lung
Published: May 2022

In the central nervous system (CNS), the matrix metalloproteinase-9 (MMP-9) is induced by several factors and contributes to CNS disorders, including inflammation and neurodegeneration. Thus, the upregulation of MMP-9 has been considered to be an indicator of inflammation. Interleukin-1β (IL-1β) is an important proinflammatory cytokine which can induce various inflammatory factors, such as MMP-9, in many inflammatory disorders. Several phytochemicals are believed to reduce the risk of several inflammatory disorders, including the CNS diseases. Among them, the resveratrol, a principal phenolic compound of the grape, blueberry, and mulberry peels and Cassia plants, has been shown to possess several medicinal properties, including antioxidative, anti-inflammatory, and antitumor function. Herein, we used mouse-brain microvascular endothelial cells (bMECs) to demonstrate the signaling mechanisms of IL-1β-induced MMP-9 expression via zymographic, RT-PCR, Western blot, reactive oxygen species (ROS) detection, immunofluorescence stain, and promoter reporter analyses. Then we evaluated the effects of resveratrol on IL-1β-induced MMP-9 expression in bMECs and its mechanism of action. We first demonstrated that IL-1β induced MMP-9 expression in bMECs. Subsequently, IL-1β induced MMP-9 expression via ROS-mediated c-Src-dependent transactivation of EGFR, and then activation of the ERK1/2, p38 MAPK, JNK1/2, and NF-κB signaling pathway. Finally, we determined that IL-1β-induced upregulation of MMP-9 may cause the disruption of the arranged integrity of zonula occludens-1 (ZO-1), but this could be inhibited by resveratrol. These data indicated that resveratrol may have antioxidative and brain-protective activities by reducing these related pathways of ROS-mediated MMP-9 expression and tight junction disruption in brain microvascular endothelial cells.
Modulation of Rxrα Expression in Mononuclear Phagocytes Impacts on Cardiac Remodeling after Ischemia-Reperfusion Injury
Authors: Räuber Saskia , Fischer Maximilian , Messerer Denise , Wimmler Vanessa , Konda Kumaraswami , Todica Andrei , Lorenz Michael , Titova Anna , Schulz Christian , Weinberger Tobias
Published: May 2022

Retinoid X receptors (RXRs), as members of the steroid/thyroid hormone superfamily of nuclear receptors, are crucial regulators of immune response during health and disease. RXR subtype expression is dependent on tissue and cell type, RXRα being the relevant isoform in monocytes and macrophages. Previous studies have assessed different functions of RXRs and positive implications of RXR agonists on outcomes after ischemic injuries have been described. However, the impact of a reduced Rxrα expression in mononuclear phagocytes on cardiac remodeling after myocardial infarction (MI) has not been investigated to date. Here, we use a temporally controlled deletion of Rxrα in monocytes and macrophages to determine its role in ischemia-reperfusion injury. We show that reduced expression of Rxrα in mononuclear phagocytes leads to a decreased phagocytic activity and an accumulation of apoptotic cells in the myocardium, reduces angiogenesis and cardiac macrophage proliferation in the infarct border zone/infarct area, and has an impact on monocyte/macrophage subset composition. These changes are associated with a greater myocardial defect 30 days after ischemia/reperfusion injury. Overall, the reduction of Rxrα levels in monocytes and macrophages negatively impacts cardiac remodeling after myocardial infarction. Thus, RXRα might represent a therapeutic target to regulate the immune response after MI in order to improve cardiac remodeling.
Deciphering the Role of Histone Modifications in Uterine Leiomyoma: Acetylation of H3K27 Regulates the Expression of Genes Involved in Proliferation, Cell Signaling, Cell Transport, Angiogenesis and Extracellular Matrix Formation
Authors: Carbajo-García María Cristina , de Miguel-Gómez Lucia , Juárez-Barber Elena , Trelis Alexandra , Monleón Javier , Pellicer Antonio , Flanagan James M. , Ferrero Hortensia
Published: May 2022

Uterine leiomyoma (UL) is a benign tumor arising from myometrium (MM) with a high prevalence and unclear pathology. Histone modifications are altered in tumors, particularly via histone acetylation which is correlated with gene activation. To identify if the acetylation of H3K27 is involved in UL pathogenesis and if its reversion may be a therapeutic option, we performed a prospective study integrating RNA-seq (n = 48) and CHIP-seq for H3K27ac (n = 19) in UL vs MM tissue, together with qRT-PCR of SAHA-treated UL cells (n = 10). CHIP-seq showed lower levels of H3K27ac in UL versus MM (p-value < 2.2 × 10−16). From 922 DEGs found in UL vs. MM (FDR < 0.01), 482 presented H3K27ac. A differential acetylation (FDR < 0.05) was discovered in 82 of these genes (29 hyperacetylated/upregulated, 53 hypoacetylated/downregulated). Hyperacetylation/upregulation of oncogenes (NDP,HOXA13,COL24A1,IGFL3) and hypoacetylation/downregulation of tumor suppressor genes (CD40,GIMAP8,IL15,GPX3,DPT) altered the immune system, the metabolism, TGFβ3 and the Wnt/β-catenin pathway. Functional enrichment analysis revealed deregulation of proliferation, cell signaling, transport, angiogenesis and extracellular matrix. Inhibition of histone deacetylases by SAHA increased expression of hypoacetylated/downregulated genes in UL cells (p < 0.05). Conclusively, H3K27ac regulates genes involved in UL onset and maintenance. Histone deacetylation reversion upregulates the expression of tumor suppressor genes in UL cells, suggesting targeting histone modifications as a therapeutic approach for UL.
EpisomiR, a New Family of miRNAs, and Its Possible Roles in Human Diseases
Authors: Arao Yasuko , Nakayama Mika , Tsuji Yoshiko , Hamano Yumiko , Otsuka Chihiro , Vecchione Andrea , Ofusa Ken , Ishii Hideshi
Published: May 2022

MicroRNAs (miRNAs) are synthesized through a canonical pathway and play a role in human diseases, such as cancers and cardiovascular, neurodegenerative, psychiatric, and chronic inflammatory diseases. The development of sequencing technologies has enabled the identification of variations in noncoding miRNAs. These miRNA variants, called isomiRs, are generated through a non-canonical pathway, by several enzymes that alter the length and sequence of miRNAs. The isomiR family is, now, expanding further to include episomiRs, which are miRNAs with different modifications. Since recent findings have shown that isomiRs reflect the cell-specific biological function of miRNAs, knowledge about episomiRs and isomiRs can, possibly, contribute to the optimization of diagnosis and therapeutic technology for precision medicine.
From a Chemotherapeutic Drug to a High-Performance Nanocatalyst: A Fast Colorimetric Test for Cisplatin Detection at ppb Level
Authors: Mastronardi Valentina , Moglianetti Mauro , Ragusa Edoardo , Zunino Rodolfo , Pompa Pier Paolo
Published: May 2022

A rapid point-of-care method for the colorimetric detection of cisplatin was developed, exploiting the efficient conversion of the chemotherapeutic drug into a high-performance nanocatalyst with peroxidase enzyme mimics. This assay provides high specificity and ppb-detection sensitivity with the naked eye or a smartphone-based readout, outperforming many standard laboratory-based techniques. The nanocatalyst-enabled colorimetric assay can be integrated with machine-learning methods, providing accurate quantitative measurements. Such a combined approach opens interesting perspectives for the on-site monitoring of both chemotherapeutic patients to achieve optimal treatments and healthcare workers to prevent their unsafe exposure.
Fear of Losing Jobs during COVID-19: Can Psychological Capital Alleviate Job Insecurity and Job Stress?
Authors: Peng Bangxin , Potipiroon Wisanupong
Published: May 2022

Job insecurity is a growing concern among bank employees. In this research, we examined whether psychological capital can alleviate bank workers’ perceptions of job insecurity and job stress during COVID-19. In particular, we aimed to examine this relationship at both the individual and work-unit levels of analysis. Based on the data collected from 520 bank tellers in 53 bank branches in Thailand, our multilevel structural equation modeling (MSEM) analyses revealed that there was significant between-branch variation in the levels of psychological capital (15%), job insecurity (23%) and job stress (24%). In particular, perceptions of job insecurity were found to have a positive effect on job stress at both levels of analysis. We also found that psychological capital significantly reduced perceptions of job insecurity and job stress at the individual level. These findings emphasize that perceptions of job insecurity can emerge at both the individual and work-unit levels. Theoretical and practical implications are discussed.
The Effect of Videoconferencing on Second-Language Learning: A Meta-Analysis
Authors: Yu Li-Tang
Published: May 2022

To mitigate the unexpected closure of educational institutions during the COVID-19 pandemic, e-learning has become a practical alternative to conventional face-to-face instruction. Videoconferencing, a synchronous computer-mediated communication (SCMC) approach, has been adopted as a venue to continue student learning activities. However, in the field of second-language (L2) education, videoconferencing had already been integrated into learning tasks, enabling L2 learners to have more opportunities to access authentic linguistic input and participate in interactions with more proficient users or native speakers of the target languages. Research has reported the pedagogical benefits of learners’ L2 achievement that are provided by videoconferencing, whereas some studies have reached a different conclusion. To further ascertain the effectiveness of videoconferencing in L2 learning, meta-analysis can be used to provide statistical evidence of the significance of study results, which serves as a useful reference for the application of videoconferencing to current e-learning practices. Thus, systematic meta-analysis was used in this study to synthesize the findings from experimental and quasi-experimental research into the effectiveness of videoconferencing for L2 learning. Videoconferencing approaches led to positive, medium overall effects in control/experimental group comparisons (g = 0.35, p < 0.5) on the L2 language development of listening and speaking abilities. However, this conclusion is based on five studies and, thus, needs to be treated cautiously. The implications of the findings and suggestions for future studies are discussed.
Refurbishing Classrooms for Hybrid Learning: Balancing between Infrastructure and Technology Improvements
Authors: Marey Ahmed , Goubran Sherif , Tarabieh Khaled
Published: May 2022

The COVID-19 pandemic has posed significant challenges to all facets of education. As students are slowly repopulating university campuses after lockdowns and online learning, universities are looking into ways to ensure social distancing can be maintained in learning spaces and capitalize on the benefits of online-learning modalities without compromising educational quality. One option that has gained attention is hybrid or dual-delivery learning. In this model, some students are present in classrooms, while others join the class through online platforms. However, most university classrooms are not designed to deliver the voice of instructors and classroom students to online platforms. This change in modality requires universities to invest in infrastructure and technology. This research studies the optimum setup for dual-delivery classrooms, investigating a range of infrastructural and technology improvements that can be made to traditional classrooms to help optimize the perceived sound quality for remote learners. The investigation entails a qualitative study to assess the improvement in perceived sound quality (clarity, ability to recognize words, and perceived echo) for remote students vis-a-vis the improvement in the room (such as sound-absorbing padding or carpets) and its technology (such as ceiling and lapel microphones). Specifically, it investigates the degrees of room and the technology improvements needed to ensure that the voice of instructors and the classroom students’ interactions, such as questions and discussions, are heard and comprehended by remote students. We collected responses for nine experiment conditions through 39 tests addressing both instruction and students’ interactions. We formulate a matrix of recommendations for higher education institutions to follow. The suggestions proposed also have other environmental benefits beyond sound quality.
One-Step Biodiesel Production from Waste Cooking Oil Using CaO Promoted Activated Carbon Catalyst from Prunus persica Seeds
Authors: Hameed Ayesha , Naqvi Salman Raza , Sikandar Umair , Chen Wei-Hsin
Published: May 2022

In recent years, the scope for replacing fossil fuels has been appealing to the world, owing to limited conventional fuels, crude oil price volatility, and greenhouse gas emission concerns. In this regard, this article demonstrates the preparation of a novel solid base catalyst for the transesterification of waste cooking oil. A calcium-loaded activated carbon catalyst was prepared through pyrolysis of peach shell followed by chemical activation with KOH and then calcium loading through the wet impregnation method. The prepared catalyst showed the best performance with 20% calcium loading and 650 °C of calcination temperature. The catalyst’s physicochemical, structural, and textural properties were examined using XRD, FTIR, SEM, EDX, and BET analysis. The catalyst showed a maximum yield of 96% at optimized conditions, i.e., 65 °C temperature, oil to methanol ratio 1:8, 5 wt% catalyst concentration, and a 160 min reaction time. Additionally, it illustrated high recyclability up to 10 cycles with negligible leaching of Ca+2 ions. The high activity of the catalyst was due to the presence of calcium ions on the activated carbon support. Physio–chemical properties and GC-MS analysis of prepared biodiesel determined that all attributes were within the biodiesel standard tolerances set by ASTM D6751 and EN 14214. Therefore, all the innovations mentioned above concluded that catalyst generated from peach shell biochar is a promising candidate for biodiesel production, ultimately resulting in solid and liquid waste management.
Advances in Designing Efficient La-Based Perovskites for the NOx Storage and Reduction Process
Authors: Zhao Dongyue , Song Haitao , Liu Jun , Jiang Qiuqiao , Li Xingang
Published: May 2022

To overcome the inherent challenge of NOx reduction in the net oxidizing environment of diesel engine exhaust, the NOx storage and reduction (NSR) concept was proposed in 1995, soon developed and commercialized as a promising DeNOx technique over the past two decades. Years of practice suggest that it is a tailor-made technique for light-duty diesel vehicles, with the advantage of being space saving, cost effective, and efficient in NOx abatement; however, the over-reliance of NSR catalysts on high loadings of Pt has always been the bottleneck for its wide application. There remains fervent interest in searching for efficient, economical, and durable alternatives. To date, La-based perovskites are the most explored promising candidate, showing prominent structural and thermal stability and redox property. The perovskite-type oxide structure enables the coupling of redox and storage centers with homogeneous distribution, which maximizes the contact area for NOx spillover and contributes to efficient NOx storage and reduction. Moreover, the wide range of possible cationic substitutions in perovskite generates great flexibility, yielding various formulations with interesting features desirable for the NSR process. Herein, this review provides an overview of the features and performances of La-based perovskite in NO oxidation, NOx storage, and NOx reduction, and in this way comprehensively evaluates its potential to substitute Pt and further improve the DeNOx efficiency of the current NSR catalyst. The fundamental structure–property relationships are summarized and highlighted to instruct rational catalyst design. The critical research needs and essential aspects in catalyst design, including poisoner resistance and catalyst sustainability, are finally addressed to inspire the future development of perovskite material for practical application.
Coupling LaNiO3 Nanorods with FeOOH Nanosheets for Oxygen Evolution Reaction
Authors: Sun Minghong , Zhang Qicheng , Chen Qiming , Hou Xiaohan , Peng Wenchao , Li Yang , Zhang Fengbao , Xia Qing , Fan Xiaobin
Published: May 2022

Perovskite-based electrocatalysts with compositional flexibility and tunable electronic structures have emerged as one of the promising non-noble metal candidates for oxygen evolution reaction (OER). Here, we propose a heterostructure comprising perovskite oxide (LaNiO3) nanorods and iron oxide hydroxide (FeOOH) nanosheets as an effective electrochemical catalyst for OER. The optimized 0.25Fe-LNO catalyst with an interesting 1D-2D hierarchical structure shows a low overpotential of 284 mV at 10 mA cm−2 and a small Tafel slope of 69 mV dec−1. The enhanced performance can be explained by the synergistic effect between LaNiO3 and FeOOH, resulting in an improved electrochemically active surface area, facilitated charge transfer and the optimized adsorption of OH intermediates.
The CXCL12/CXCR4/ACKR3 Signaling Axis Regulates PKM2 and Glycolysis
Authors: Luker Kathryn E. , Luker Gary D.
Published: May 2022

In response to CXCL12, CXCR4 and ACKR3 both recruit β-arrestin 2, regulating the assembly of interacting proteins that drive signaling and contribute to the functions of both receptors in cancer and multiple other diseases. A prior proteomics study revealed that β-arrestin 2 scaffolds pyruvate kinase M2 (PKM2), an enzyme implicated in shifting cells to glycolytic metabolism and poor prognosis in cancer. We hypothesized that CXCL12 signaling regulates PKM2 protein interactions, oligomerization, and glucose metabolism. We used luciferase complementation in cell-based assays and a tumor xenograft model of breast cancer in NSG mice to quantify how CXCR4 and ACKR3 change protein interactions in the β-arrestin-ERK-PKM2 pathway. We also used mass spectrometry to analyze the effects of CXCL12 on glucose metabolism. CXCL12 signaling through CXCR4 and ACKR3 stimulated protein interactions among β-arrestin 2, PKM2, ERK2, and each receptor, leading to the dissociation of PKM2 from β-arrestin 2. The activation of both receptors reduced the oligomerization of PKM2, reflecting a shift from tetramers to dimers or monomers with low enzymatic activity. Mass spectrometry with isotopically labeled glucose showed that CXCL12 signaling increased intermediate metabolites in glycolysis and the pentose phosphate pathway, with ACKR3 mediating greater effects. These data establish how CXCL12 signaling regulates PKM2 and reprograms cellular metabolism.
CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
Authors: Pannunzio Bruno , Amo-Aparicio Jesús , Julián Camila , López-Vales Rubèn , Peluffo Hugo , Lago Natalia
Published: May 2022

Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 in Wallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery.
LINCing Senescence and Nuclear Envelope Changes
Authors: Meqbel Bakhita R. M. , Gomes Matilde , Omer Amr , Gallouzi Imed E. , Horn Henning F.
Published: May 2022

The nuclear envelope (NE) has emerged as a nexus for cellular organization, signaling, and survival. Beyond its role as a barrier to separate the nucleoplasm from the cytoplasm, the NE’s role in supporting and maintaining a myriad of other functions has made it a target of study in many cellular processes, including senescence. The nucleus undergoes dramatic changes in senescence, many of which are driven by changes in the NE. Indeed, Lamin B1, a key NE protein that is consistently downregulated in senescence, has become a marker for senescence. Other NE proteins have also been shown to play a role in senescence, including LINC (linker of nucleoskeleton and cytoskeleton) complex proteins. LINC complexes span the NE, forming physical connections between the cytoplasm to the nucleoplasm. In this way, they integrate nuclear and cytoplasmic mechanical signals and are essential not only for a variety of cellular functions but are needed for cell survival. However, LINC complex proteins have been shown to have a myriad of functions in addition to forming a LINC complex, often existing as nucleoplasmic or cytoplasmic soluble proteins in a variety of isoforms. Some of these proteins have now been shown to play important roles in DNA repair, cell signaling, and nuclear shape regulation, all of which are important in senescence. This review will focus on some of these roles and highlight the importance of LINC complex proteins in senescence.
Human Sperm Morphology as a Marker of Its Nuclear Quality and Epigenetic Pattern
Authors: Bendayan Marion , Caceres Liliana , Saïs Emine , Swierkowski-Blanchard Nelly , Alter Laura , Bonnet-Garnier Amélie , Boitrelle Florence
Published: May 2022

Background: Human sperm chromatin condensation is a sum of epigenetic events that allows for the near-complete replacement of histones with protamines. Under high-magnification microscopy, nuclear vacuoles have been described as thumbprints with poor chromatin condensation. The objective of this study is to examine whether vacuolated spermatozoa carry specific epigenetic marks, which may influence embryo development. Methods: The presence and three-dimensional distribution of ten epigenetic marks (protamine-P2, histone-H3, H3K4me1/me2/me3, H3K9me1/me2/me3, H3K27me3, H4k20me2) were evaluated and compared in morphometrically normal spermatozoa according to the presence or absence of a large vacuole occupying more than 15% of the head surface (n = 4193). Results: Vacuolated spermatozoa were significantly more frequently labelled with H3 and H3K4me3 than normal spermatozoa (88.1% ± 2.7 and 78.5% ± 5.2 vs. 74.8% ± 4.8 and 49.1% ± 7.4, respectively; p = 0.009 and p < 0.001) and significantly less marked by P2 and H3K27me3 (50.2% ± 6.2 and 63.9% ± 6.3 vs. 82.1% ± 4.4 and 73.6% ± 5.1, respectively; p < 0.001 and p = 0.028). In three dimensions, vacuoles are nuclear concavities filled with DNA carrying the H3K4me3 marker. Conclusion: High-magnification microscopy is a simple tool to estimate in real time the sperm epigenetic profile. The selection of normal spermatozoa without vacuoles and the deselection of spermatozoa with vacuoles appear to be epigenetically favorable to embryo development and safe offspring.