bluuebunny/crossref_metadata_2025 huggingface dataset:
A Bibliometric Analysis of Wearable Device Research Trends 2001–2022—A Study on the Reversal of Number of Publications and Research Trends in China and the USA
Authors: Kageyama Itsuki , Kurata Karin , Miyashita Shuto , Lim Yeongjoo , Sengoku Shintaro , Kodama Kota
Published: December 2022

In recent years, Wearable Devices have been used in a wide variety of applications and fields, but because they span so many different disciplines, it is difficult to ascertain the intellectual structure of this entire research domain. No review encompasses the whole research domain related to Wearable Devices. In this study, we collected articles on wearable devices from 2001 to 2022 and quantitatively organized them by bibliometric analysis to clarify the intellectual structure of this research domain as a whole. The cluster analysis, co-occurrence analysis, and network centrality analysis were conducted on articles collected from the Web of Science. As a result, we identified one cluster that represents applied research and two clusters that represent basic research in this research domain. Furthermore, focusing on the top two countries contributing to this research domain, China and the USA., it was confirmed that China is extremely inclined toward basic research and the USA. toward applied research, indicating that applied and basic research are in balance. The basic intellectual structure of this cross-sectional research domain was identified. The results summarize the current state of research related to Wearable Devices and provide insight into trends.
Acceptance and Commitment Therapy for Destructive Experiential Avoidance (ACT-DEA): A Feasibility Study
Authors: Na Euihyeon , Lee KangUk , Jeon Bong-Hee , Jo Cheolrae , Kwak Uk-Hwan , Jeon Yujin , Yang Kyojin , Lee Eui Jin , Jeong Jin
Published: December 2022

Background: This study is a preliminary study on an acceptance and commitment therapy (ACT) program that mitigates destructive experiential avoidance (DEA) behaviors, including self-harm behavior and addiction; Methods: Twenty participants aged 15–25 years who had confirmed DEA behavior within the last month participated in a total of six sessions of ACT. Demographic characteristics, history of psychiatric illness, and TYPES and patterns of DEA behavior were confirmed in the baseline survey. The severity of clinical symptoms, frequency of DEA behavior and impulsivity, characteristics of experiential avoidance (EA) behavior, depression, and quality of life (QOL) were measured before and after the program for comparative statistical tests using the intention-to-treat method. Furthermore, the severity of clinical symptoms was evaluated after each program, along with the frequency of DEA behavior and trends in impulsivity, which were investigated based on the behavior log; Results: After the ACT program, both the frequency of DEA behavior and impulsivity and the severity of clinical symptoms, depression, and anxiety decreased significantly. Furthermore, among the EA characteristics, pain aversion, distraction and inhibition, and delayed behavior significantly improved. Moreover, the overall QOL, psychological and social relationships, and QOL regarding the environment also improved; Conclusions: The results of this feasibility study demonstrate the potential of the ACT program as an effective intervention in DEA behavior. The results of this study may be used as preliminary data for future large-scale randomized studies.
Gender Matters: Identity, Risk Perception and Preventive Interventions for Alcohol Consumption among Adolescents Using a Qualitative Approach
Authors: Martínez-Manrique Lucía , Berasaluce Maitane , Sureda Xisca , Sandín Vázquez María
Published: December 2022

The epidemiological information available in Spain and the Community of Madrid highlights two relevant facts regarding alcohol consumption: an increase in binge drinking in teenagers and a reduction/reversal of the gender gap, particularly at young ages. This article aims to describe some of the factors related to alcohol use in teenagers, especially those related to gender and risk perception. A qualitative study was designed with semi-structured interviews and a discussion group with students from the city of Madrid aged 14 to 18 years. A descriptive analysis of the content of the replies of 28 teenagers was conducted. The results show that alcohol consumption has an identity component, both in terms of transition to adulthood and gender role performance. Consumption is also associated with risks, especially those determined by gender inequality, which teenagers learn to manage as a means of survival in nightlife. Preventive campaigns typically lack a gender perspective and a focus on risk prevention. To reduce the prevalence of consumption and associated risks, these strategies need to be reformulated with a gender perspective.
Buprenorphine Microdosing Cross Tapers: A Time for Change
Authors: Raheemullah Amer , Benhamou Ori-Michael , Kuo Jamie , Lembke Anna
Published: December 2022

Buprenorphine is a partial opioid agonist that is Food and Drug Administration (FDA) approved to treat chronic pain and opioid use disorder (OUD). The national prescribing guidelines in the United States (US) recommend that patients transitioning from full opioid agonists to buprenorphine first undergo 12 or more hours of active opioid withdrawal, in order to avoid buprenorphine-precipitated opioid withdrawal. This opioid-free period imposes a significant barrier for many patients. Evidence is accumulating that using microdoses of buprenorphine to cross taper from full-agonist opioids to buprenorphine is a safe and effective way to avoid opioid withdrawal and uncontrolled pain. This microdose cross-tapering strategy is already being used across the US. The US prescribing guidelines and buprenorphine training would benefit from acknowledging this new approach. Additionally, to facilitate this strategy, the FDA should approve transdermal buprenorphine formulations for OUD and manufacturers could produce lower dose formulations of sublingual buprenorphine. The time has come for us to embrace buprenorphine microdosing cross tapers as a new standard of care.
Multi-Dimensional Dynamics of Psychological Health Disparities under the COVID-19 in Japan: Fairness/Justice in Socio-Economic and Ethico-Political Factors
Authors: Kobayashi Masaya , Ishido Hikari , Mizushima Jiro , Ishikawa Hirotaka
Published: December 2022

This article addresses citizens’ psychological health disparities in pandemic-stricken Japan from the perspective of positive psychology with a collective/political perspective. Our analysis of three internet surveys in 2020 and 2021 in Japan indicates most people’s well-being declined continuously during this period, while some people’s well-being increased. As previous studies of health inequality proved about physical health, the objective income/assets level has influenced psychological inequality. This paper demonstrated this relation in Japan, although it is often mentioned as an egalitarian country with comparatively better health conditions. Moreover, psychological levels and changes have been associated with biological, natural environmental, cultural, and social factors. Social factors include economic, societal-community, and political factors, such as income/assets, stratification, general trust, and fairness/justice. Accordingly, multi-dimensional disparities are related to psychological health disparity; tackling the disparities along the multi-layered strata is desirable. Furthermore, subjective perception of fairness/justice is significantly associated with the level of psychological health and mitigating its decrease. Thus, fairness and justice are found to be dynamic and protective factors against the decline of psychological health. While relatively little literature on health inequality analyzes fairness/justice philosophically, this paper highlights these together with income/assets by clarifying the significance of multi-dimensional factors: natural environmental, cultural, socioeconomic, and political.
Occupational Safety and Health with Technological Developments in Livestock Farms: A Literature Review
Authors: Hayden Marie A. , Barim Menekse S. , Weaver Darlene L. , Elliott K. C. , Flynn Michael A. , Lincoln Jennifer M.
Published: December 2022

In recent decades, there have been considerable technological developments in the agriculture sector to automate manual processes for many factors, including increased production demand and in response to labor shortages/costs. We conducted a review of the literature to summarize the key advances from installing emerging technology and studies on robotics and automation to improve agricultural practices. The main objective of this review was to survey the scientific literature to identify the uses of these new technologies in agricultural practices focusing on new or reduced occupational safety risks affecting agriculture workers. We screened 3248 articles with the following criteria: (1) relevance of the title and abstract with occupational safety and health; (2) agriculture technologies/applications that were available in the United States; (3) written in English; and (4) published 2015–2020. We found 624 articles on crops and harvesting and 80 articles on livestock farming related to robotics and automated systems. Within livestock farming, most (78%) articles identified were related to dairy farms, and 56% of the articles indicated these farms were using robotics routinely. However, our review revealed gaps in how the technology has been evaluated to show the benefits or potential hazards to the safety and well-being of livestock owners/operators and workers.
Live Streaming vs. Pre-Recorded Training during the COVID-19 Pandemic in Italian Rhythmic Gymnastics
Authors: Cardinali Ludovica , Curzi Davide , Maccarani Emanuela , Falcioni Lavinia , Campanella Matteo , Ferrari Dafne , Maulini Claudia , Gallotta Maria Chiara , Zimatore Giovanna , Baldari Carlo , Guidetti Laura
Published: December 2022

The SARS-CoV-2 outbreak led to an inevitable and drastic transition to online training systems. This study aimed to analyze the differences between live-streaming and pre-recorded training modalities in rhythmic gymnastics (RG) with coaches and gymnasts of different technical levels. A total of 238 coaches and 277 athletes affiliated with the Italian Gymnastics Federation (FGI) participated in the study. The data collection tool was a self-collected questionnaire structured in three sections: participant’s information, characteristics of live-streaming training, and characteristics of pre-recorded training. A 2 × 2 ANOVA was used for each numeric variable. A Pearson’s chi-squared test was used for each categorical variable. For the athletes, training frequency, motivation, and efficacy were significantly higher (p < 0.05) with live streaming (3.7 ± 1.5 day/week, 3.8 ± 0.9 score and 3.8 ± 0.8 score, respectively) than with a pre-recorded modality (2.2 ± 1.7 day/week, 3.1 ± 1.2 score and 3.7 ± 0.9 score, respectively), while for coaches, significant differences (p < 0.005) were found between the two modalities only for training frequency (live streaming, 3.6 ± 1.8 days/week vs. pre-recorded, 2.1 ± 1.7 days/week). The adherence (number of No:Yes) was significantly higher (p < 0.05) for the live-streaming modality than for the pre-recorded modality in gold athletes (1:74 vs. 14:61, respectively), silver athletes (12:190 vs. 28:174, respectively), and gold coaches (3:63 vs. 11:55, respectively), but it was not for silver coaches. Physical preparation was performed significantly (p < 0.005) more often (number of No:Yes) for live streaming than for the pre-recorded modality by gold athletes (9:66 vs. 34:41, respectively), silver athletes (25:177 vs. 77:125, respectively), gold coaches (8:58 vs. 37:29), and silver coaches (33:139 vs. 85:87, respectively). Free body technical preparation was performed significantly (p < 0.005) more often (number of No:Yes) for live streaming than for the pre-recorded modality by gold athletes (15:60 vs. 39:36, respectively), silver athletes (84:118 vs. 121:81, respectively), gold coaches (10:56 vs. 38:28), and silver coaches (60:112 vs. 105:67, respectively), while no differences were found for silver athletes’ and coaches’ technical preparations for apparatus training between the two modalities. In conclusion, live streaming had greater positive effects on RG training during home confinement. However, pre-recorded training could be more effective for some types of training, depending on the technical level of the athletes.
Interchangeability between the Data Obtained by Two Powermeters during Road Cycling Competitions: A Case Study
Authors: Iglesias-Pino Javier , Herrero-Molleda Alba , Fernández-Fernández Jaime , García-López Juan
Published: December 2022

Various power meters are used to assess road-cycling performance in training and competition, but no previous study has analyzed their interchangeability in these conditions. Therefore, the purpose was to compare the data obtained from two different power meters (PowerTap vs. Power2Max) during cycling road races. A national-level under-23 male competitive cyclist completed six road-cycling official competitions (five road races and one individual time trial), in which power output was simultaneously registered with the two power meters. After this, the main power output variables were analyzed with the same software. The average and critical power obtained from the PowerTap power meter were slightly lower than from the Power2Max power meter (3.56 ± 0.68 and 3.62 ± 0.74 W·kg−1, 5.06 and 5.11 W·kg−1, respectively), and the correlations between both devices were very high (r ≥ 0.996 and p < 0.001). In contrast, the PowerTap power meter registered a significantly higher (p < 0.05) percentage of time at <0.75 and >7.50 W·kg−1 and power profile at 1, 5 and 10 s. In conclusion, the data obtained in competitions by the two power meters were interchangeable. Nevertheless, the Power2Max power meter underestimated the pedaling power during short and high-intensity intervals (≤10.0 s and >7.50 W·kg−1) compared to the PowerTap power meter. Therefore, the analysis of these efforts should be treated with caution.
Healthcare Services for Undocumented Migrants: Organisation and Costs from the Italian NHS Perspective
Authors: Listorti Elisabetta , Torbica Aleksandra , Cella Silvano G. , Fiorini Gianfrancesco , Corrao Giovanni , Franchi Matteo
Published: December 2022

Based on the principle of health equity, the Italian National Health Service is known worldwide for being a universalistic system that guarantees healthcare services for all its population, among which there are undocumented migrants. A commitment for their health needs is further motivated by their lower utilisation rates of healthcare services, which becomes even more crucial when considering chronic conditions such as diabetes that require adherence and continuity of care. However, the need for more official data has resulted in little research documenting these healthcare usage patterns. For this reason, our objective has been to deepen, from the Italian NHS perspective, the quantity, costs, type, preventability and organisation of healthcare services directed to undocumented migrants. We used official healthcare data from the Lombardy Region, which enable the identification of people receiving the STP code (undocumented migrants) and of people with foreign citizenship (documented migrants). After quantifying the average annual amount and expenditure for healthcare services grouped by Italian citizens, documented migrants and undocumented migrants for all clinical conditions (quantity and costs), we performed three primary investigations where we enlightened differences between the three mentioned groups focusing on the diagnosis of diabetes: (i) mapping the types of healthcare services used and their characteristics (type); (ii) quantifying the impact of preventable hospital admissions (preventability); (iii) examining the healthcare patterns linking pharmaceutical prescriptions with hospital accesses (organisation). Our results reveal significant differences among the three groups, such as more urgent hospital admissions, more preventable complications, and a higher recurrence in terms of access and costs to hospital services rather than pharmaceutical prescriptions for undocumented migrants. These findings can represent the leverage to raise awareness toward the emerging challenges of the migrant health burden.
Managing Oral Health in the Context of Antimicrobial Resistance
Authors: Bessa Lucinda J. , Botelho João , Machado Vanessa , Alves Ricardo , Mendes José João
Published: December 2022

The oral microbiome plays a major role in shaping oral health/disease state; thus, a main challenge for dental practitioners is to preserve or restore a balanced oral microbiome. Nonetheless, when pathogenic microorganisms install in the oral cavity and are incorporated into the oral biofilm, oral infections, such as gingivitis, dental caries, periodontitis, and peri-implantitis, can arise. Several prophylactic and treatment approaches are available nowadays, but most of them have been antibiotic-based. Given the actual context of antimicrobial resistance (AMR), antibiotic stewardship in dentistry would be a beneficial approach to optimize and avoid inappropriate or even unnecessary antibiotic use, representing a step towards precision medicine. Furthermore, the development of new effective treatment options to replace the need for antibiotics is being pursued, including the application of photodynamic therapy and the use of probiotics. In this review, we highlight the advances undergoing towards a better understanding of the oral microbiome and oral resistome. We also provide an updated overview of how dentists are adapting to better manage the treatment of oral infections given the problem of AMR.
Evaluation of Biodegradation of BTEX in the Subsurface of a Petrochemical Site near the Yangtze River, China
Authors: Chen Xuexia , Zhang Shuai , Yi Lijin , Liu Zhengwei , Ye Xiangyu , Yu Bo , Shi Shuai , Lu Xiaoxia
Published: December 2022

The contamination of soil and groundwater with BTEX (benzene, toluene, ethyl benzene, and xylenes) is a common issue at petrochemical sites, posing a threat to the ecosystems and human health. The goal of this study was to evaluate the biodegradation of BTEX in the subsurface of a petrochemical site near the Yangtze River, thus providing scientific basis for bioremediation of the contaminated site. Both molecular analysis of field samples and microcosm study in the laboratory were performed for the evaluation. Soil and groundwater samples were collected from the site. Microcosms were constructed with inoculum from the soil and incubated anaerobically in the presence of nitrate, ferric oxide, manganese oxide, sulfate, and sodium bicarbonate, respectively. The initial concentration of each component of BTEX (benzene, toluene, ethyl benzene, o-xylene) was 4–5 mg/L. Actinobacteria was dominant in the highly contaminated soil, while Proteobacteria was dominant in the slightly contaminated soil and the groundwater. The relative abundances of Firmicutes, Spirochaetes, and Caldiserica were higher in the highly contaminated soil and groundwater samples compared to those in the corresponding slightly contaminated samples. The relative abundances of predicted functions, such as carbohydrate transport and metabolism, nucleotide transport and metabolism, coenzyme transport and metabolism, amino acid transport and metabolism, etc., in the highly contaminated soil and groundwater samples were higher than those in the corresponding slightly contaminated samples. In microcosms, biodegradations of BTEX occurred, and the first-order rate constants in the presence of various electron acceptors had the following order: sulfate (0.08–0.10/d) > sodium bicarbonate (0.07–0.09/d) > ferric oxide (0.04–0.06/d) > nitrate (0.03–0.05/d) > manganese oxide (0.01–0.04/d).
Effect of Fermented Sarco Oyster (Crassostrea gigas) Extract on Muscle Strength Enhancement in Postmenopausal Females: A Randomized, Double-Blind, Placebo-Controlled Trial
Authors: Rheu Kyoung-Min , Lee Bae-Jin , Son Woo-Hyeon , Kim Dong-Seok , Park Hyun-Tae , Ha Min-Seong , Gong Byong-Hak , Jeon Byeong-Hwan
Published: December 2022

A randomized controlled trial (RCT) was conducted to evaluate the effect of fermented sarco oysters (FSO) on muscle strength in postmenopausal females with low muscle mass. Fifty-two female participants were randomly divided into the experiment group (EG) or control group (CG). For 12 weeks, the EG was subjected to 1000 mg of FSO extract daily while the CG consumed the placebo extract. The muscle extension and flexion at an angular velocity of 60°/s and with respect to grip strength, body composition, and muscle growth-related blood factors were measured at the baseline and after the trial. The difference in the quadriceps muscle extension at an angular velocity of 60°/s, grip strength on both the left and right side, and insulin-like growth factor-1(IGF-1) between groups were significantly higher in the EG compared with the CG. However, no differences were found in body composition, blood pyruvate, lactate, or high-sensitivity C-reactive protein (hsCRP) concentration between the two groups. In conclusion, FSO supplements may improve muscle strength in postmenopausal females with relatively reduced muscle strength without a change in muscle mass.
Relationship between Intervertebral Disc Compression Force and Sagittal Spinopelvic Lower Limb Alignment in Elderly Women in Standing Position with Patient-Specific Whole Body Musculoskeletal Model
Authors: Miura Takanori , Hongo Michio , Kasukawa Yuji , Kijima Hiroaki , Kudo Daisuke , Saito Kimio , Kimura Ryota , Iwami Takehiro , Miyakoshi Naohisa
Published: December 2022

The intervertebral disc loading based on compensated standing posture in patients with adult spinal deformity remains unclear. We analyzed the relationship between sagittal alignment and disc compression force (Fm). In 14 elderly women, the alignment of the sagittal spinopelvic and lower extremities was measured. Fm was calculated using the Anybody Modeling System. Patients were divided into low sagittal vertical axis (SVA) and high SVA groups. Comparisons between the two groups were performed and the relationship between the Fm and each parameter was examined using Spearman’s correlation coefficient (r). The mean lumbar Fm in the high SVA group was 67.6%; significantly higher than that in the low SVA group (p = 0.046). There was a negative correlation between cervical Fm with T1 slope (r = −0.589, p = 0.034) and lumbar Fm with lumbar lordosis (r = −0.566, p = 0.035). Lumbar Fm was positively correlated with center of gravity-SVA (r = 0.615, p = 0.029), T1 slope (r = 0.613, p = 0.026), and SVA (r = 0.612, p = 0.020). The results suggested sagittal malalignment increased the load on the thoracolumbar and lower lumbar discs and was associated with cervical disc loading.
Research on the Relationship between Physical Literacy, Physical Activity and Sedentary Behavior
Authors: Yan Wenjing , Meng Yihan , Wang Lina , Zhang Ting , Chen Leqin , Li Hongjuan
Published: December 2022

During the COVID-19 pandemic, college students’ health-related physical activity and physical literacy aroused widespread concern. This study evaluated the relationship among physical literacy (PL), sedentary behavior (SB), light physical activity (LPA), and moderate-to-vigorous physical activity (MVPA); we further explored whether LAP and SB mediated the association between PL and MVPA. Methods: This study was based on a cross-sectional survey of Chinese college students. The Perceived Physical Literacy Instrument Scale (PPLI-SC) and International Physical Activity Questionnaire Short Form (IPAQ-SF) were used to investigate the PL, MVPA, LPA, and SB. Results: There were 2996 valid questionnaires with 829 boys and 2167 girls. The MVPA, LPA, and PL of boys were significantly higher than girls, while the SB values were significantly lower in girls (p < 0.01). The correlation analysis showed that there was a significant correlation between the two indexes except for SB and LPA. Path analysis shows that PL directly, significantly, and positively affects MVPA. PL reduces SB (β = −0.085, p < 0.001) and increases LPA (β = 0.097, p < 0.001). The total mediation effect accounted for 14.014%, and the mediation effects of SB and LPA accounted for 4.417% and 9.597%, respectively. Conclusions: LPA and SB partially mediated the relation between PL and MVPA. SB and LPA partially explain the impact of PL on MVPA. The findings suggest that managing SB and improving LPA could play a significant indirect role in increasing the positive effect of PL on MVPA and that increasing the opportunities for LPA increased the MVPA for Chinese college students.
Impact of “Three Red Lines” Water Policy (2011) on Water Usage Efficiency, Production Technology Heterogeneity, and Determinant of Water Productivity Change in China
Authors: Shah Wasi Ul Hassan , Lu Yuting , Hao Gang , Yan Hong , Yasmeen Rizwana
Published: December 2022

This research evaluates the effects of the Three Red Lines policy on water usage efficiency (WUE), production technology heterogeneity, and water productivity change in 31 Chinese provinces between 2006 and 2020. SMB-DEA, Meta-frontier analysis, and Malmquist–Luenberger index (MLI) techniques were employed for estimation. Results revealed that the mean WUE (2006–2020) in all Chinese provinces was 0.52, with an improvement potential of 48%. Shanghai, Beijing, Shaanxi, and Tianjin were the best performers. The WUE scores before (2006–2011) and after (2012–2020) water policy implementation were 0.58 and 0.48, respectively; on average, there was more than a 9% decline in WUE after the implementation of the water policy. The eastern region has the most advanced water utilization technology as its technology gap ratio (TGR) is nearly 1. The average MLI (2006–2020) score was 1.13, suggesting that the MLI has increased by 12.57% over the study period. Further technology change (TC) is the key predictor of MLI growth, whereas efficiency change (EC) diminished from 2006 to 2020. The mean MLI score for 2006–2011 was 1.16, whereas the MLI Score for the period 2012–2020 was 1.10, indicating a modest decline following the implementation of the water policy. All three Chinese regions experienced MLI growth during 2006–2020, with TC the main change factor.
Exposure to Inorganic Arsenic in Rice in Brazil: A Human Health Risk Assessment
Authors: Toledo Michele C. , Lee Janice S. , Batista Bruno L. , Olympio Kelly P. K. , Nardocci Adelaide C.
Published: December 2022

In certain populations, rice is the main source of exposure to inorganic arsenic (iAs), which is associated with cancer and non-cancer effects. Although rice is a staple food in Brazil, there have been few studies about the health risks for the Brazilian population. The objective of this study was to assess the risks of exposure to iAs from white rice and brown rice in Brazil, in terms of the carcinogenic and non-carcinogenic effects, and to propose measures to mitigate those risks. The incremental lifetime cancer risk (ILCR) and hazard quotient (HQ) were calculated in a probabilistic framework. The mean ILCR was 1.5 × 10−4 for white rice and 6.0 × 10−6 for brown rice. The HQ for white and brown rice was under 1. The ILCR for white and brown rice was high, even though the iAs concentration in rice is below the maximum contaminant level. The risk for brown rice consumption was lower, which was not expected. Various mitigation measures discussed in this report are estimated to reduce the risk from rice consumption by 5–67%. With the support of public policies, measures to reduce these risks for the Brazilian population would have a positive impact on public health.
Factors Affecting Willingness to Receive Online Counseling: The Mediating Role of Ethical Concerns
Authors: Chen Xin , Du Anzheng , Qi Rufang
Published: December 2022

Since the outbreak of the COVID-19 pandemic, traditional face-to-face counseling has gradually given way to online counseling. To improve the application value of online counseling and change the current situation of college students’ lack of willingness to receive online counseling, this study explored factors that influence Chinese college students’ willingness to receive online counseling (WROC). Based on data gathered from surveying 823 Chinese college students using self-report questionnaires, we clarified the relationships between the self-stigma of seeking help, ethical concerns about online counseling (ECOC), online interpersonal trust (OIT), and the willingness to receive online counseling (WROC). The results indicated that (1) self-stigma of seeking help and OIT negatively and positively predicted the WROC, respectively; (2) ethical concerns negatively predicted the WROC; and (3) ethical concerns mediated the relationship between self-stigma and WROC and between OIT and WROC. The results suggest that reducing the self-stigma surrounding help-seeking, perfecting the ethical norms of online counseling, and enhancing interpersonal trust can improve willingness to receive online counseling.
Job Accessibility as a Lens for Understanding the Urban Structure of Colonial Cities: A Digital Humanities Study of the Colonial Seoul in the 1930s Using GIS
Authors: Kim Youngjoon , Kim Junghwan , Ha Hui Jeong , Nakajima Naoto , Lee Jinhyung
Published: December 2022

This study examined the urban structure of colonial Seoul in the 1930s, the capital city of Korea under the rule of the Japanese empire, by adopting quantitative geographical methods. We utilized a job accessibility index to operationalize the urban structure. We also used geographic information science (GIScience) analysis tools to digitize neighborhood-level sociodemographic and parcel-level business location information from historical materials. The results illustrated several findings that were not revealed by previous studies based on qualitative approaches. First, transit-based job accessibility (13.392) is significantly higher (p < 0.001) than walk-based job accessibility (10.575). Second, there is a Γ-shaped area with higher job accessibility, including the central part of colonial Seoul. Third, Japanese-dominant neighborhoods had significantly (p < 0.001) higher transit-based (27.156) job accessibility than Korean-dominant neighborhoods (9.319). Fourth, transit-based job accessibility is not significantly correlated with the unemployment rate overall. Although colonial Seoul was the seventh-largest city of the Japanese empire, few practical planning actions were taken to resolve urban issues, unlike the other large cities in mainland Japan.
Predicting N6-Methyladenosine Sites in Multiple Tissues of Mammals through Ensemble Deep Learning
Authors: Luo Zhengtao , Lou Liliang , Qiu Wangren , Xu Zhaochun , Xiao Xuan
Published: December 2022

N6-methyladenosine (m6A) is the most abundant within eukaryotic messenger RNA modification, which plays an essential regulatory role in the control of cellular functions and gene expression. However, it remains an outstanding challenge to detect mRNA m6A transcriptome-wide at base resolution via experimental approaches, which are generally time-consuming and expensive. Developing computational methods is a good strategy for accurate in silico detection of m6A modification sites from the large amount of RNA sequence data. Unfortunately, the existing computational models are usually only for m6A site prediction in a single species, without considering the tissue level of species, while most of them are constructed based on low-confidence level data generated by an m6A antibody immunoprecipitation (IP)-based sequencing method, thereby restricting reliability and generalizability of proposed models. Here, we review recent advances in computational prediction of m6A sites and construct a new computational approach named im6APred using ensemble deep learning to accurately identify m6A sites based on high-confidence level data in multiple tissues of mammals. Our model im6APred builds upon a comprehensive evaluation of multiple classification methods, including four traditional classification algorithms and three deep learning methods and their ensembles. The optimal base–classifier combinations are then chosen by five-fold cross-validation test to achieve an effective stacked model. Our model im6APred can produce the area under the receiver operating characteristic curve (AUROC) in the range of 0.82–0.91 on independent tests, indicating that our model has the ability to learn general methylation rules on RNA bases and generalize to m6A transcriptome-wide identification. Moreover, AUROCs in the range of 0.77–0.96 were achieved using cross-species/tissues validation on the benchmark dataset, demonstrating differences in predictive performance at the tissue level and the need for constructing tissue-specific models for m6A site prediction.
Impacts of Telomeric Length, Chronic Hypoxia, Senescence, and Senescence-Associated Secretory Phenotype on the Development of Thoracic Aortic Aneurysm
Authors: Aschacher Thomas , Geisler Daniela , Lenz Verena , Aschacher Olivia , Winkler Bernhard , Schaefer Anne-Kristin , Mitterbauer Andreas , Wolf Brigitte , Enzmann Florian K. , Messner Barbara , Laufer Günther , Ehrlich Marek P. , Grabenwöger Martin , Bergmann Michael
Published: December 2022

Thoracic aortic aneurysm (TAA) is an age-related and life-threatening vascular disease. Telomere shortening is a predictor of age-related diseases, and its progression is associated with premature vascular disease. The aim of the present work was to investigate the impacts of chronic hypoxia and telomeric DNA damage on cellular homeostasis and vascular degeneration of TAA. We analyzed healthy and aortic aneurysm specimens (215 samples) for telomere length (TL), chronic DNA damage, and resulting changes in cellular homeostasis, focusing on senescence and apoptosis. Compared with healthy thoracic aorta (HTA), patients with tricuspid aortic valve (TAV) showed telomere shortening with increasing TAA size, in contrast to genetically predisposed bicuspid aortic valve (BAV). In addition, TL was associated with chronic hypoxia and telomeric DNA damage and with the induction of senescence-associated secretory phenotype (SASP). TAA-TAV specimens showed a significant difference in SASP-marker expression of IL-6, NF-κB, mTOR, and cell-cycle regulators (γH2AX, Rb, p53, p21), compared to HTA and TAA-BAV. Furthermore, we observed an increase in CD163+ macrophages and a correlation between hypoxic DNA damage and the number of aortic telocytes. We conclude that chronic hypoxia is associated with telomeric DNA damage and the induction of SASP in a diseased aortic wall, promising a new therapeutic target.
Screening of Candidate Genes Associated with Brown Stripe Resistance in Sugarcane via BSR-seq Analysis
Authors: Cheng Wei , Wang Zhoutao , Xu Fu , Lu Guilong , Su Yachun , Wu Qibin , Wang Ting , Que Youxiong , Xu Liping
Published: December 2022

Sugarcane brown stripe (SBS), caused by the fungal pathogen Helminthosporium stenospilum, is one of the most serious threats to sugarcane production. However, its outbreaks and epidemics require suitable climatic conditions, resulting in the inefficient improvement of the SBS resistance by phenotype selection. The sugarcane F1 population of SBS-resistant YT93-159 × SBS-susceptible ROC22 was used for constructing the bulks. Bulked segregant RNA-seq (BSR-seq) was then performed on the parents YT93-159 (T01) and ROC22 (T02), and the opposite bulks of 30 SBS-susceptible individuals mixed bulk (T03) and 30 SBS-resistant individuals mixed bulk (T04) collected from 287 F1 individuals. A total of 170.00 Gb of clean data containing 297,921 SNPs and 70,426 genes were obtained. Differentially expressed genes (DEGs) analysis suggested that 7787 and 5911 DEGs were identified in the parents (T01 vs. T02) and two mixed bulks (T03 vs. T04), respectively. In addition, 25,363 high-quality and credible SNPs were obtained using the genome analysis toolkit GATK for SNP calling. Subsequently, six candidate regions with a total length of 8.72 Mb, which were located in the chromosomes 4B and 7C of sugarcane wild species Saccharum spontaneum, were identified, and 279 genes associated with SBS-resistance were annotated by ED algorithm and ΔSNP-index. Furthermore, the expression profiles of candidate genes were verified by quantitative real-time PCR (qRT-PCR) analysis, and the results showed that eight genes (LRR-RLK, DHAR1, WRKY7, RLK1, BLH4, AK3, CRK34, and NDA2) and seven genes (WRKY31, CIPK2, CKA1, CDPK6, PFK4, CBL2, and PR2) of the 20 tested genes were significantly up-regulated in YT93-159 and ROC22, respectively. Finally, a potential molecular mechanism of sugarcane response to H. stenospilum infection is illustrate that the activations of ROS signaling, MAPK cascade signaling, Ca2+ signaling, ABA signaling, and the ASA-GSH cycle jointly promote the SBS resistance in sugarcane. This study provides abundant gene resources for the SBS resistance breeding in sugarcane.
MicroRNA Modulation during Orthodontic Tooth Movement: A Promising Strategy for Novel Diagnostic and Personalized Therapeutic Interventions
Authors: Cultrera Giovanni , Lo Giudice Antonino , Santonocito Simona , Ronsivalle Vincenzo , Conforte Cristina , Reitano Giuseppe , Leonardi Rosalia , Isola Gaetano
Published: December 2022

The Orthodontic Tooth Movement (OTM) is allowed through a mediated cell/tissue mechanism performed by applying a force or a pair of forces on the dental elements, and the tooth movement is a fundamental requirement during any orthodontic treatment. In this regard, it has been widely shown that each orthodontic treatment has a minimum duration required concerning numerous factors (age, patient compliance, type of technique used, etc.). In this regard, the aim of the following revision of the literature is to give readers a global vision of principal microRNAs (miRNAs) that are most frequently associated with OTM and their possible roles. Previously published studies of the last 15 years have been considered in the PubMed search using “OTM” and “miRNA” keywords for the present review article. In vitro and in vivo studies and clinical trials were mainly explored. Correlation between OTM and modulation of several miRNAs acting through post-transcriptional regulation on target genes was observed in the majority of previous studied. The expression analysis of miRNAs in biological samples, such as gingival crevicular fluid (GCF), can be considered a useful tool for novel diagnostic and/or prognostic approaches and for new personalized orthodontic treatments able to achieve a better clinical response rate. Although only a few studies have been published, the data obtained until now encourage further investigation of the role of miRNA modulation during orthodontic treatment. The aim of this study is to update the insights into the role and impact of principal micro-RNAs (miRNAs) that are most frequently associated during OTM.
Fecal Luminal Factors from Patients with Gastrointestinal Diseases Alter Gene Expression Profiles in Caco-2 Cells and Colonoids
Authors: Holst Luiza , Iribarren Cristina , Sapnara Maria , Savolainen Otto , Törnblom Hans , Wettergren Yvonne , Strid Hans , Simrén Magnus , Magnusson Maria K. , Öhman Lena
Published: December 2022

Previous in vitro studies have shown that the intestinal luminal content, including metabolites, possibly regulates epithelial layer responses to harmful stimuli and promotes disease. Therefore, we aimed to test the hypothesis that fecal supernatants from patients with colon cancer (CC), ulcerative colitis (UC) and irritable bowel syndrome (IBS) contain distinct metabolite profiles and establish their effects on Caco-2 cells and human-derived colon organoids (colonoids). The metabolite profiles of fecal supernatants were analyzed by liquid chromatography–mass spectrometry and distinguished patients with CC (n = 6), UC (n = 6), IBS (n = 6) and healthy subjects (n = 6). Caco-2 monolayers and human apical-out colonoids underwent stimulation with fecal supernatants from different patient groups and healthy subjects. Their addition did not impair monolayer integrity, as measured by transepithelial electrical resistance; however, fecal supernatants from different patient groups and healthy subjects altered the gene expression of Caco-2 monolayers, as well as colonoid cultures. In conclusion, the stimulation of Caco-2 cells and colonoids with fecal supernatants derived from CC, UC and IBS patients altered gene expression profiles, potentially reflecting the luminal microenvironment of the fecal sample donor. This experimental approach allows for investigating the crosstalk at the gut barrier and the effects of the gut microenvironment in the pathogenesis of intestinal diseases.
Sphingosine as a New Antifungal Agent against Candida and Aspergillus spp.
Authors: Hashemi Arani Fahimeh , Kadow Stephanie , Kramer Melanie , Keitsch Simone , Kirchhoff Lisa , Schumacher Fabian , Kleuser Burkhard , Rath Peter-Michael , Gulbins Erich , Carpinteiro Alexander
Published: December 2022

This study investigated whether sphingosine is effective as prophylaxis against Aspergillus spp. and Candida spp. In vitro experiments showed that sphingosine is very efficacious against A. fumigatus and Nakeomyces glabrataa (formerly named C. glabrata). A mouse model of invasive aspergillosis showed that sphingosine exerts a prophylactic effect and that sphingosine-treated animals exhibit a strong survival advantage after infection. Furthermore, mechanistic studies showed that treatment with sphingosine leads to the early depolarization of the mitochondrial membrane potential (Δψm) and the generation of mitochondrial reactive oxygen species and to a release of cytochrome C within minutes, thereby presumably initiating apoptosis. Because of its very good tolerability and ease of application, inhaled sphingosine should be further developed as a possible prophylactic agent against pulmonary aspergillosis among severely immunocompromised patients.
Modified mRNA Therapeutics for Heart Diseases
Authors: Magadum Ajit
Published: December 2022

Cardiovascular diseases (CVD) remain a substantial global health problem and the leading cause of death worldwide. Although many conventional small-molecule treatments are available to support the cardiac function of the patient with CVD, they are not effective as a cure. Among potential targets for gene therapy are severe cardiac and peripheral ischemia, heart failure, vein graft failure, and some forms of dyslipidemias. In the last three decades, multiple gene therapy tools have been used for heart diseases caused by proteins, plasmids, adenovirus, and adeno-associated viruses (AAV), but these remain as unmet clinical needs. These gene therapy methods are ineffective due to poor and uncontrolled gene expression, low stability, immunogenicity, and transfection efficiency. The synthetic modified mRNA (modRNA) presents a novel gene therapy approach which provides a transient, stable, safe, non-immunogenic, controlled mRNA delivery to the heart tissue without any risk of genomic integration, and achieves a therapeutic effect in different organs, including the heart. The mRNA translation starts in minutes, and remains stable for 8–10 days (pulse-like kinetics). The pulse-like expression of modRNA in the heart induces cardiac repair, cardiomyocyte proliferation and survival, and inhibits cardiomyocyte apoptosis post-myocardial infarction (MI). Cell-specific (cardiomyocyte) modRNA translation developments established cell-specific modRNA therapeutics for heart diseases. With these laudable characteristics, combined with its expression kinetics in the heart, modRNA has become an attractive therapeutic for the treatment of CVD. This review discusses new developments in modRNA therapy for heart diseases.
Reducing Pre- and Post-Treatments in Cryopreservation Protocol and Testing Storage at −80 °C for Norway Spruce Embryogenic Cultures
Authors: Varis Saila A. , Virta Susanna , Montalbán Itziar A. , Aronen Tuija
Published: December 2022

Somatic embryogenesis (SE) is considered the most effective method for vegetative propagation of Norway spruce (Picea abies L. Karst). For mass propagation, a storage method that is able to handle large quantities of embryogenic tissues (ETs) reliably and at a low cost is required. The aim of the present study was to compare freezing at −80 °C in a freezer to cryopreservation using liquid nitrogen (LN) as a method for storing Norway spruce ETs. The possibility of simplifying both the pre-treatment and thawing processes in cryopreservation was also studied. The addition of abscisic acid (ABA) to the pre-treatment media and using polyethylene glycol PEG4000 instead of PEG6000 in a cryoprotectant solution were tested. Both the pre-and post-treatments on semi-solid media could be simplified by reducing the number of media, without any loss of genotype or embryo production capacity of ETs. On the contrary, the storage of ETs in a freezer at −80 °C instead of using LN was not possible, and the addition of ABA to the pre-treatment media did not provide benefits but increased costs. The lower regeneration rate after using PEG4000 instead of PEG6000 in a cryoprotectant solution in cryovials was unexpected and unwanted. The simplified pre-and post-treatment protocol will remarkably reduce the workload and costs in the mass-cryopreservation of future forest regeneration materials and in thawing the samples for mass propagations, respectively.
Novel Approaches in Molecular Imaging and Neuroimaging of Fibromyalgia
Authors: Ricci Maria , Cimini Andrea , Grivet Fojaja Maria Rosaria , Ullo Mariacristina , Carabellese Bruno , Frantellizzi Viviana , Lubrano Ennio
Published: December 2022

Fibromyalgia (FM) represents a condition that is still controversial in its entity, pathophysiology, diagnosis and management. The aim of this review is to focus on imaging aspects of FM, especially on novel approaches in molecular imaging, with a special focus on neuroimaging. Novel functional and molecular imaging findings may represent, eventually, future biomarkers both in research settings and in terms of clinical practice. Several imaging techniques have already been tested in clinical trials in the FM field, including functional MRI, positron emission tomography (PET) imaging with 18F-FDG in FM, PET imaging of the dopaminergic system, PET imaging of the GABAergic system, PET imaging with neuroinflammation and neuroimmune parameters, PET imaging of the opioid system and H215O-PET activation studies. Therefore, the potential role in the FM field of fMRI and different PET tracers has been discussed in different settings, serving as a comprehensive guide of novel imaging options both in research and in the clinical field.