bluuebunny/crossref_metadata_2025 huggingface dataset:
162 Digestibility of energy and nutrients and concentration of metabolizable energy in soybean expellers and soybean meal fed to growing pigs
Authors: Rodriguez Diego A , Lee Su A , Stein Hans H
Published: November 2020

Two experiments were conducted to test the hypothesis that energy and nutrient digestibility and concentrations of DE and ME are greater in soybean expellers than in soybean meal (SBM) fed to growing pigs. In Exp. 1, two diets based on soybean expellers or SBM as the sole source of AA and an N-free diet were formulated. Nine cannulated barrows (BW: 55.98 ± 13.75 kg) were allotted to a triplicated 3 × 3 Latin square design with 3 diets and 3 periods. Ileal digesta were collected on d 6 and 7 of each 7-d period. The statistical model included ingredient as fixed effect and square, period, and animal as random effects. Results indicated that the standardized ileal digestibility (SID) of most AA were greater (P < 0.05) or tended to be greater (P < 0.10) in soybean expellers compared with SBM (Table 1). In Exp. 2, a corn-based diet and 2 diets based on corn and each of the 2 soybean products were formulated. Twenty-four barrows (BW: 44.88 ± 2.17 kg) were allotted to 1 of the 3 diets with 8 pigs per diet. Urine and fecal samples were collected for 4 d after 5 d of adaptation. The statistical model included diet or ingredient as fixed effect and replicate as random effect. Results indicated that the apparent total tract digestibility (ATTD) of GE was not different among pigs fed the 3 diets. Concentrations of DE and ME in soybean expellers were greater (P < 0.05) compared with SBM, but the ATTD of total dietary fiber was not different between the 2 soybean products. In conclusion, values for SID of most AA and the DE and ME in soybean expellers were greater than in SBM.
156 Influence of viscosity and fiber on nutrient digestibility and intestinal physiology in growing pigs
Authors: Hung Yuan-Tai , Zhu Jinlong , Shurson Gerald C , Saqui-Salces Milena , Urriola Pedro E
Published: November 2020

Fiber is known for decreasing nutrient utilization in pigs. The addition of fiber alters diet viscosity, but our understanding of the effect of diet viscosity on nutrient digestibility is limited. This knowledge is necessary for improving dietary fiber utilization and when evaluating alternative feed ingredients. Thirty-six T-cannulated barrows (n = 6/treatment; initial BW = 26.5 ± 3.9 kg) were fed either corn-soybean meal (CSBM) basal diets or CSBM with 30% distillers dried grains with solubles as fiber source (CSBM+DDGS). The viscosity of diets were modified by using non-viscous cellulose (CEL), medium-viscosity carboxymethylcellulose (MCMC), or high-viscosity carboxymethylcellulose (HCMC) to assess viscosity effects on nutrient digestibility and intestinal physiology. After 29 d on diets, ileal digesta were collected to determine viscosity and apparent ileal digestibility (AID). Also, intestinal tissue and contents were harvested to determine morphometry, goblet cell quantitation, and digestive enzymatic activity. Data were analyzed using a linear mixed model with treatments as fixed effects and initial BW as a random effect. Results are shown in Table 1. No interactions were observed between viscosity and diets. Inclusion of MCMC and HCMC increased viscosity of supernatant and whole digesta compared with CEL. Increasing diet viscosity decreased AID of DM and CP in pigs fed MCMC and HCMC compared with pigs fed CEL, respectively. The AID of DM and CP were greater in CSBM than CSBM+DDGS. Inclusion of CMC increased crypt depth in the jejunum and goblet cell area in the ileum compared with pigs fed CEL regardless of basal diet fed. Digesta trypsin activity was not different among treatments, but inclusion of CMC increased amylase activity by 43% in jejunal digesta compared with CEL. The addition of DDGS reduced AID; however, viscosity affected intestinal morphology and function, suggesting that viscosity is the main characteristics of fiber affecting nutrient utilization of pigs.
178 Efficacy of commercial products on growth performance of nursery pigs fed diets with fumonisin-contaminated corn
Authors: Rao Zhong-Xing , Tokach Mike D , Dritz Steve S , Woodworth Jason C , DeRouchey Joel M , Goodband Robert D , Calderon Cartagena Hilda I
Published: November 2020

Two experiments were conducted to determine the efficacy of commercial products on growth performance of nursery pigs fed high fumonisin diets. In Exp. 1,350 pigs (241 × 600; DNA; initially 9.9 kg) were used with 5 pigs per pen and 14 pens per treatment. Five dietary treatments consisted of a positive control (low fumonisin, 4 ppm fumonisin; FB1 + FB2), negative control (50 ppm fumonisin;) and the negative control with one of three products (0.3% of Kallsil Dry, Kemin Industries Inc., Des Moines, IA; 0.3% of Feed Aid Wide Spectrum, NutriQuest, Mason City, IA; 0.17% of Biofix Select Pro, Biomin America Inc., Overland Park, KS). Diets were fed for 14 d. Pens were assigned to treatments in a randomized complete block design with initial weight as the blocking factor. Data were analyzed using nlme package in R program (version 3.5.2) with pen as experimental unit. Pigs fed the negative control, or diets with Kallsil Dry or Feed Aid had decreased (P< 0.05) ADG, ADFI, and G:F compared with those fed the positive control and diet with Biofix. Pigs fed the positive control diet had decreased (P< 0.05) d 14 serum sphinganine to sphingosine (Sa:So) ratio than those fed other diets. In Exp. 2, 300 pigs (241 × 600; initially 10.4 kg) were used and fed experimental diets for 28 d. Procedures were similar to Exp. 1 except there were 12 replicates per treatment and diets contained 30 ppm fumonisin. Pigs fed the negative control, or diets with Kallsil Dry or Feed Aid had decreased (P< 0.05) ADG and G:F, and greater (P< 0.05) d 14 and 28 Sa:So ratios compared with the positive control and diet with Biofix. In summary, adding Biofix to high fumonisin diets mitigated the negative effects of fumonisin while Kallsil Dry and Feed Aid did not.
176 Evaluation of a novel threonine source for nursery pig diets
Authors: Humphrey Dalton , Becker Spenser , Lee Jason , Haydon Keith , Greiner Laura L
Published: November 2020

Four hundred and eighty (PIC 337 X 1050, PIC Genus, Hendersonville, TN) pigs were used to evaluate a novel threonine source (ThrPro, CJ America-Bio, Fort Dodge, IA) for nursery pigs from approximately 7 kg to 20 kg. At weaning, pigs were sorted by gender and fed a common diet for one week. Upon completion of the first week, pigs were sorted into randomized complete blocks, equalized by weight, within 16 replications. Pigs were allocated to one of three dietary treatments: positive control (POS)-SID LYS:THR 0.60, negative control (NEG)-SID LYS:THR ≤0.46 and alternative threonine source (TEST)-SID LYS:THR 0.60. All other nutrients met or exceeded the NRC (2012) recommendations. Growth and intake data were analyzed using PROC MIXED procedure in SAS. The experimental unit was the pen. During the first 14 days, pigs fed TEST had similar ADG (0.417 vs. 0.414 kg/d, P=0.81) and G:F (0.977 vs. 1.030, P=0.18) compared to POS, and increased ADG (0.417 vs. 0.387 kg/d, P=0.01) and G:F (0.977 vs. 0.898, P=0.05) compared to NEG. Over days 14-28, pigs fed TEST had similar ADG (0.523 vs. 0.532 kg/d, P=0.49) and G:F (0.712 vs. 0.707, P=0.71) compared to POS, and increased ADG (0.523 vs. 0.479 kg/d, P=0.002) and G:F (0.712 vs. 0.627, P< 0.0001) compared to NEG. Overall (d 0 to 28), pigs fed TEST had similar ADG (0.466 vs. 0.474 kg/d, P=0.48) and G:F (0.808 vs. 0.816, P=0.55) compared to POS, and increased ADG (0.466 vs. 0.433 kg/d, P=0.002) and G:F (0.808 vs. 0.725, P< 0.0001) compared to NEG. ADFI was not significantly different across treatments for the entirety of the study. In conclusion, the replacement of L-threonine with a novel threonine source resulted in similar growth performance in nursery pigs from approximately 7 kg to 20 kg.
266 Dietary strategies in diets without zinc oxide for nursery pigs
Authors: Laskoski Fernanda , Hutchens Wade M , Tokach Mike D , Woodworth Jason C , Goodband Robert D , Dritz Steve S , DeRouchey Joel M
Published: November 2020

A total of 300 pigs (DNA 241 × 600; initially 4.2 kg) were used to evaluate the effects of different nutritional strategies to replace zinc oxide (ZnO) in nursery diets. Treatments consisted of A) Positive control (21% CP; ZnO providing 3,000 ppm Zn from d 0 to 7, and 2,000 ppm Zn from d 7 to 25); B) Negative control (NC; no ZnO); C) NC plus 1.2% Na diformate; D) NC with 4% coarse ground wheat bran; E) NC with low crude protein (18%) by adding high levels of feed grade amino acids; and F) the combination of NC with 18% crude protein (CP), 1.2% Na diformate, and 4% coarse ground wheat bran. There were 5 pigs per pen and 10 pens per treatment in a completely randomized design. Data were analyzed with the Proc Mixed procedure in SAS. For the overall period (d 0 to 46), pigs fed diets containing ZnO had greater (P < 0.001) ADG, ADFI, and final BW than other treatments, with pigs fed the NC with 18% CP having the lowest performance. Pigs fed the NC diet without any nutritional alternative had the lowest fecal dry matter (DM; P < 0.050) and highest fecal scores (P < 0.050). Pigs fed diets with ZnO had similar fecal DM and scores as pigs fed the diet containing the combination of 1.2% Na diformate, 4% coarse ground wheat bran, and low (18%) CP, but greater (P < 0.023) fecal DM than pigs fed the alternatives used individually. This study suggests none of the strategies used alone were effective at replacing ZnO while the use of low CP and amino acids reduced performance further. Combining the three alternatives tested as a replacement to ZnO improved fecal DM content and fecal score but did not result in improved growth performance.
275 Feeding a whole-cell inactivated Pichia guilliermondi yeast to gestating and lactating sows in a commercial production system
Authors: Thayer Morgan T , Garcia Ricardo M , Duttlinger Alan W , Mahoney Julie F , Schinckel Allan P , Asmus Matthew D , Jones Daniel B , Dunn James L , Richert Brian T
Published: November 2020

A total of 606 sows (PIC 1050) and their progeny (PIC 1050×280) were used to determine if feeding gestating and lactating sows a proprietary strain of Pichia guilliermondi as a whole-cell inactivated yeast product (WCY; CitriStim, ADM Animal Nutrition, Quincy, IL) improves sow and litter performance in a commercial production system. Once confirmed pregnant at d 35 post-breeding pregnancy check, sows were fed either a basal gestation control (CON) diet (0.55% SID Lysine) or the control diet fortified with 0.15% (1.36 kg/ton) of the WCY replacing corn in the CON diet. Dietary treatments were also fed in lactation (1.05% SID Lysine) once sows were moved into farrowing crates on approximately d 112 of gestation until weaning. Sows supplemented with WCY in gestation and lactation had a greater number of total born piglets by 0.45 pigs (P=0.040), born alive (P< 0.04), heavier born alive litter weight (P< 0.001), and greater post cross-foster litter size (P< 0.001) compared to CON fed sows. Litter size at weaning was increased by 0.54 pigs when sows were fed WCY compared to CON (P< 0.001). However, the average piglet weaning weights from CON fed sows were heavier by 0.35 kg compared to WCY (P< 0.001). This increase in body weight of piglets from CON fed sows is partially explained by their 0.93 days longer lactation (P< 0.001), and may also be due to the smaller litter size nursed throughout lactation. The percent of litters treated for scours decreased from 38.3% to 14.2% when sows were fed WCY (P< 0.001). The distribution of birth weights by body weight range was not different (P=0.2461) between treatments. In conclusion, feeding gestating and lactating sows a proprietary strain of Pichia guilliermondi as a whole-cell inactivated yeast product increased the number of piglets born and number weaned as well as decreased the prevalence of scours during lactation.
287 Effects of copper hydroxychloride on growth performance and abundance of genes involved in lipid metabolism of growing pigs
Authors: Espinosa Charmaine , Fry Robert Scott , Kocher Matthew , Stein Hans H
Published: November 2020

An experiment was conducted to test the hypothesis that Cu hydroxychloride (IntelliBond CII, Micronutrients, LLC, Indianapolis, IN) improves growth performance by upregulating the mRNA transcription of genes involved in lipid metabolism of pigs. Thirty-two pigs (15.05 ± 0.98 kg) were allotted to 2 dietary treatments with 2 pigs per pen for a total of 8 replicate pens per treatment. Pigs were fed a control diet based on corn, soybean meal, and distillers dried grains with solubles that included Cu to meet the requirement. A second diet was formulated by adding 150 mg Cu/kg from Cu hydroxychloride to the control diet. On the last day of the experiment, one pig per pen was sacrificed and samples from liver, skeletal muscle, and subcutaneous adipose tissue were collected to analyze relative mRNA abundance of genes involved in lipid metabolism. Data were analyzed using SAS with pen as the experimental unit. Diet was the fixed effect and replicate was the random effect. Results indicated that overall average daily gain and gain:feed were greater (P < 0.05) for pigs fed the diet containing Cu hydroxychloride compared with pigs fed the control diet (Table 1). Pigs fed the diet supplemented with Cu hydroxychloride had increased (P < 0.05) abundance of cluster of differentiation 36 in liver and increased (P < 0.05) abundance of fatty acid binding protein 4 and lipoprotein lipase in subcutaneous adipose tissue. Inclusion of Cu hydroxychloride also tended to increase (P < 0.10) abundance of fatty acid binding protein 1, peroxisome proliferator-activated receptor alpha, and carnitine palmitoyl transferase 1 B in liver, skeletal muscle, and subcutaneous adipose tissue, respectively. In conclusion, supplementation of Cu hydroxychloride to the control diet improved growth performance and may affect signaling pathways associated with lipid metabolism by upregulating abundance of some genes involved in post-absorptive metabolism of lipids.
289 Effects of supranutritional levels of dietary zinc oxide on zinc, copper, and iron metabolism in post-weaned pigs
Authors: Dalto Danyel Bueno , Guay Frédéric , Martel-Kennes Yan , Talbot Guylaine , Lessard Martin , Matte Jacques , Lapointe Jérôme
Published: November 2020

This study evaluated the impact of supranutritional levels of dietary zinc oxide (ZnO) on zinc (Zn), copper (Cu), and iron (Fe) homeostasis in post-weaned pigs. One hundred twenty piglets were selected at d14 of age (5.65 ± 0.68 kg). At weaning (d21), they were fed a basal post-weaning diet (without antibiotics) supplemented (n = 40/treatment) with 100 mg/kg (low; LZn), 1000 mg/kg (medium; MZn) or 3000 mg/kg (high; HZn) of ZnO until d42. Dietary levels of Cu (130 mg/kg) and Fe (260 mg/kg) remained constant throughout the experimental period. Piglets were sacrificed at d21, d23, d35, and d42 of age (n = 10/treatment/d) for samples collection. Serum concentrations and total liver content of Zn and Cu were not different among treatments on d21 and d23 (P ≥ 0.10). For Zn, treatment effects were detected at d35 and d42 (P < 0.01) on both serum concentration and total Zn content in liver. For both parameters, values were greatest for HZn, intermediary for MZn, and lowest for LZn irrespective of ages in the two periods. For Cu, treatment effects were detected at d35 and d42 (P < 0.01) on both serum concentration and total Cu content in liver. For both parameters, values were greatest for LZn and MZn and lowest for HZn irrespective of ages. For Fe, no treatment effect was detected either on whole blood Fe concentrations (P = 0.81) or hemoglobin levels (P = 0.76). However, for total Fe content in liver, treatment effects were detected (P < 0.01). Values were greatest for LZn and lowest for HZn at d35 and d42 whereas for MZn they were intermediary at d35 and similar to LZn at d42 (P ≤ 0.01). In conclusion, supranutritional levels of dietary ZnO fed to piglets during 3 weeks post-weaning significantly impacted Zn, Cu, and Fe homeostasis. These results emphasise the potential risk of Cu deficiency as well as impairment in building body Fe reserves under long-term supranutritional supplementation of ZnO.
292 The effect of pharmacological zinc on oral Salmonella vaccine efficacy
Authors: DeMille Carson M , Burrough Eric R , Gabler Nicholas
Published: November 2020

Pharmacological zinc (2,000-3,000 ppm) is commonly fed to nursery pigs to improve health and growth due to its antimicrobial and anti-inflammatory properties. The objective was to test if pharmacological zinc at time of oral Salmonella vaccination impeded vaccine efficacy. Sixty-four weaned pigs (5.1±0.7 kg BW) were used in a 2 x 2 factorial design. The diets were control (CON) or zinc (3,000 ppm for 1 week, 2,000 ppm for 2 weeks, and no additional zinc for 1 week [ZN]). On d 2 pigs were orally vaccinated for Salmonella with 1 of 2 commercially available vaccines, resulting in 4 treatments (CON1, CON2, ZN1, ZN2; n = 16/treatment). On d 28, n = 8 pigs/treatment were randomly selected and enrolled in a S. Typhimurium challenge study. On d 35 post-weaned, all pigs were inoculated with 108 cfu of a field S. Typhimurium isolate. Pig performance, febrile response, fecal shedding and serology was assessed over a 7-d challenge period. On dpi 7 all pigs were euthanized, and colon contents and ileocecal lymph nodes were collected for culture. The effect of nursery diet, vaccine and their interaction was assessed. Pigs were confirmed Salmonella culture positive at dpi 2 and 6 pigs were culture positive from the ileocecal lymph nodes at dpi 7. Salmonella-specific antibody titers (S/P) increased (P < 0.001) from dpi 0 (0.31) to 7 (2.01), and a time-by-vaccine interaction was reported (P < 0.05). Irrespective of diet and vaccine, core temperatures increased from 39.5°C (dpi 0) to 39.7°C (dpi 2) before decreasing (P = 0.02). Over the challenge period, ADG did not differ (0.67, 0.64, 0.61, 0.62 kg/d, CON1, CON2, ZN1, ZN2, respectively, P = 0.654). Furthermore, ADFI and G:F did not differ by diet or vaccine (P >0.05). In conclusion, pharmacological Zn did not inhibit efficacy of oral Salmonella vaccines.
27 Shotgun proteomics reveal seminal plasma proteomes are reflective of boar reproductive performance
Authors: Mills Kayla M , Aryal Uma , Sobreira Tiago , Minton Amanda , Casey Theresa , Stewart Kara R
Published: November 2020

Subfertile boars have a negative economic impact for producers and need to be identified before they enter the breeding herd. Current semen evaluation techniques are not able to identify all subfertile boars. Seminal plasma proteins are essential for normal sperm function and transport and play an important role in fertilization. We hypothesize that seminal plasma proteins affect fertility, and thus differences in protein profiles reflect differences in boar fertility. The objective of this study was to use LC-MS/MS for shotgun proteome analysis to investigate whether differences in boar fertility phenotype can be differentiated by seminal plasma protein expression. Following 50 single-sire matings, boars were categorized into one of four phenotypes: high farrowing rate and high total born (HFHB; n=9), high farrowing rate with low total born (HFLB; n=10), low farrowing rate and low total born (LFLB; n=9), and low farrowing rate with high total born (LFHB; n=4). There were 436 proteins measured in at least one sample across all animals. There were 245 high confidence proteins and 56 were differentially abundant (P < 0.05) between the high fertility phenotype (HFHB) and at least one of the three subfertile groups. Proteins associated with fertility and free radical detoxification were more abundant in HFHB whereas subfertile phenotypes had a greater abundance of blood microparticle proteins, biomarkers of inflammation, and IMPA1, which regulates inositol production. Findings support that seminal plasma protein profiles are distinct between boars with different fertility phenotypes and have the potential to predict boar reproductive performance.
1 Alternative heifer development systems utilizing corn residue and cover crops
Authors: Speer Hannah , Riley Hannah , Cushman Robert , Freetly Harvey , Drewnoski Mary
Published: November 2020

Spring-born heifers (n = 1,012) weaned at 148 ± 17 d were used in a 3-yr study to evaluate performance in winter development systems which utilized cover crop and corn residue grazing. Heifers were assigned to 1 of 3 treatments: grazing corn residue with dried distillers grains (CD) or wheat midds (CW) supplementation, or grazing late summer planted oat-brassica cover crop followed by corn residue supplemented dried distillers grains (CC). Grazing of corn residue (CD and CW) and cover crop (CC) began in early November. Supplementation during the corn residue phase was adjusted to target ~55% of mature BW (338 kg) at breeding. After 63 d, CC were moved to corn residue; on d 77 CD and CW began receiving grower ration. In mid-February (d 98), heifers were comingled and managed in a single group. Breeding season began in June and lasted for 29 d. Prior to corn residue grazing, ADG of CC was greater (0.76 kg/d; P< 0.01) than CD or CW (0.58 kg/d and 0.49 kg/d, respectively). Gain during the last 35 d of the winter period for CC was 0.13 kg/d less than CW (P< 0.01) but not different from CD. Overall winter ADG was greater (P< 0.05) for CC (0.62 kg/d) than CD (0.53 kg/d) or CW (0.50 kg/d). Percent of mature BW prior to breeding was 52% for CC and 50% for CD and CW. May reproductive tract scores did not differ (P=0.26) between CC and CW but were greater (P< 0.05) in CC than CD. Pregnancy rates were affected by treatment (P< 0.01), with CC (76%) being greater than CD (68%) and CW (64%). Utilizing oat-brassica cover crops early in the winter followed by a lower rate of gain while grazing corn residue appear to be effective for developing beef heifers. USDA is an equal opportunity employer and provider.
106 Effects of Revalor-XH and combination Revalor-IH/Revalor-200 on Yearling Heifer Growth Performance and Carcass Characteristics
Authors: Carlson Zachary , Erickson Galen E , Dicke Bill , Streeter Marshall
Published: November 2020

A commercial feedlot trial utilizing 870 crossbred heifers (initial BW = 322; SD = 9 kg) examined two implant strategies on growth performance and carcass characteristics. Treatments included: 1) Revalor-IH on d 1 (80 mg trenbalone acetate (TBA)/8 mg estradiol (E2), noncoated) and re-implanted with Revalor-200 on d 101 (200 mg TBA/20 mg E2, noncoated (REV-IH/200); or 2) Revalor-XH on d 1 (200 mg TBA/20 mg E2, partially coated (REV-XH). There were no differences (P > 0.23) observed for live performance including final BW, DMI, ADG, and feed efficiency between implant treatments. There were no differences (P ≥ 0.17) in carcass-adjusted final BW or ADG between implant strategies. There were no differences (P ≥ 0.28) observed for DMI before or after re-implanting for heifers given REV-IH/200 compared to heifers given REV-XH. Heifers implanted with REV-IH/200 had greater carcass-adjusted feed efficiency compared to REV-XH (0.156 vs. 152; P = 0.03). Heifers implanted with REV-IH/200 had larger LM area compared to heifers with REV-XH (88.4 vs. 83.9 cm2; P < 0.01). Calculated yield grade was lower for heifers implanted with REV-IH/200 compared to heifers implanted with REV-XH (3.75 vs. 3.89; P < 0.01). Hot carcass weights, dressing percent, marbling, and 12th rib back fat were not different (P ≥ 0.11) among treatments. Heifers implanted with REV-IH/200 had a shift towards lower USDA yield grade distribution compared to REV-XH implanted heifers (P = 0.05). The REV-IH/200 combination provided 280 mg of TBA and 28 mg E2, while total active ingredient dose for REV-XH was 200 mg TBA and 20 mg E2. Therefore, minimal differences were observed in carcass-adjusted feed efficiency, LM area, and calculated yield grade between REV-IH/200 and the partially coated REV-XH implant.
226 Influence of trace mineral source on digestion, ruminal volatile fatty acid and soluble mineral on steers fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow
Authors: Guimaraes Octavio , Wagner John , Spears Jerry , Engle Terry
Published: November 2020

Twelve Angus steers (BW 530.9 ± 22.7kg) fitted with ruminal cannulae were used to determine the impact of trace mineral source on digestibility, ruminal VFA, and soluble concentrations of Cu, Zn, and Mn. Steers were fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow (DM basis: 16.8% CP, 29.2 Mcal NEm, 30.3 Mcal NEl, 33.6% NDF) for 21 d. Treatments consisted of 10 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either sulfate (STM), hydroxy (HTM; IntelliBond) or organic (ORG; metal specific amino acid complexes) sources (n=4 steers/treatment). Following a 21-d adaptation period, total fecal output was collected for 5 d. On d 6, rumen fluid was collected at 0, 2, and 4 h post feeding and analyzed for VFA. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On d 15 steers received a pulse dose (cannulae) of 100 mg Cu, 400 mg Mn, and 60 mg Zn from either STM, HTM, or ORG. Ruminal samples were obtained at 2-h intervals starting at -4 and ending at 24 h post dosing and analyzed for soluble Cu, Mn, and Zn. Digestibility of NDF and ADF were lesser (P < 0.05) in STM vs. HTM and ORG supplemented steers. Steers receiving HTM and ORG had greater (P < 0.05) total VFA concentrations than STM supplemented steers at 2 and 4h post feeding. Ruminal soluble Cu and Zn concentrations were greater (P < 0.001) post dosing in STM and ORG supplemented steers at 2, 4, and 6 h for Cu and 4, 6, 8, 10 and 12 h for Zn when compared to HTM supplemented steers. Results indicate that ruminal solubility of Cu and Zn differs between STM, HTM and ORG sources and trace mineral source impacts fiber digestion and ruminal VFA concentrations.
247 The effect of diet on the microbial community structure and composition in lactating Jersey cows consuming a mixture of straw and dry distillers grains plus solubles in replacement of alfalfa hay
Authors: Knoell Allison , Aluthge Nirosh , Abbas Waseem , Bartenslager Alison , Judy Jared , Morris Dennis , Wilson Hannah C , Herrick Kevin , Kononoff Paul J , Fernando Samodha
Published: November 2020

The rumen microbial community is responsible for producing a majority of the energetic needs for the animal, yet our understanding of the rumen microbiome is in its infancy. To better understand the effect of corn-ethanol coproducts on rumen microbial communities, a replicated 4 × 4 Latin square design study utilizing 12 cows in three squares was conducted to evaluate the replacement of alfalfa hay with a mixture (CoP) containing straw and dried distillers grains plus solubles (DDGS) in lactating Jersey cows. The experimental treatments were (proportions on a dry matter basis): a control diet (CON) containing 18.2% of alfalfa hay with no straw or DDGS. A low coproduct diet (LCoP) containing 12.1% alfalfa, 2.1% straw, and 6.0% DDGS. A medium coproduct diet (MCoP) containing 6.1% alfalfa, 4.2% straw, and 12.1% DDGS. A high coproduct diet (HCoP) containing 6.2% straw, 18.1% DDGS with no alfalfa. Rumen digesta samples were collected via an esophageal tube. No differences were observed for milk production and dry matter intake (P ≥ 0.307) (mean ± SEM) 19.5 kg ± 0.60, 29.6 kg ± 0.91, across treatments, while a decrease in methane was observed (P < 0.01) for the HCoP treatment. The bacterial community was assessed by sequencing the V4 region of the 16S rRNA gene. Additionally, the archaeal community was assessed by sequencing the V4-V5 region of the 16S rRNA gene on the Illumina MiSeq platform. Amplicon Sequence Variants were identified using the DADA2 pipeline. No significant differences were observed for the bacterial (P = 0.334) and archaeal (P = 0.593) communities. Although global effects in microbial community dynamics were not observed, differential taxa were observed with Lachnospiraceae being the major differentially abundant Family. The archaeal community composition demonstrated that Methanobacteriales to be the differentially abundant Order across treatments, and may contribute to methane production.
70 Identifying factors that play a significant role in the animal science transfer student experience
Authors: Bundy Jennifer , Siberski Cori
Published: November 2020

Transfer students make up 16.5% of the undergraduate population in the Animal Science (ANS) major, and transfer enrollment is on the rise. Transfers often face challenges that are different from their direct-from-high-school peers. The objective of this study was to determine the factors that affected the transfer transition from a previous institution to the ANS department. Based on focus group (n=6) feedback, a survey instrument was developed and piloted. Using pilot data, a factor analysis was performed and the instrument was tested for reliability. Once validated, the instrument was used to collect data from first semester ANS transfer students (n=47). No incentives were offered, and participation was voluntary. Between-factor Pearson correlations were estimated, and responses to scale questions were tallied. Significant negative correlations were observed between social integration and risk of attrition (r=-0.53, p< 0.01), and between social integration and academic struggle (r=-0.48, p< 0.01). Social integration was positively correlated with overall satisfaction within the ANS department (r=0.638, p< 0.01). Students who felt socially integrated were more confident academically, more comfortable at ISU, and were less likely to leave. Students had a positive view of the ANS department if they were involved in clubs and student organizations (r=0.42, p< 0.05). Transfers who were satisfied with instructor interactions tended to be more content with the overall transfer process (r=0.44, p< 0.01). Approximately 72% of respondents felt that paying for school created a lot stress in their life, but 52% felt satisfied with the financial assistance they received. Only half of the respondents reported that the pre-registration orientation helped prepare them for transfer to ISU, but 95.9% of ANS transfers reported that they understood the requirements for graduation. These data will inform policy and procedures related to transfer student onboarding and the promotion of social interactions with peers.
71 Imitating the dynamic bovine cervix with 3D printing technology to teach artificial insemination in cattle
Authors: McCabe Esther D , Sorell Lane P , Zhang Meng , Hartman Ashley , Fike Karol E , Odde Kenneth G , Grieger David M
Published: November 2020

The process of artificial insemination in cattle is commonly taught using excised reproductive tracts. Excised reproductive tracts can be difficult to collect, and often require freezing and thawing prior to use; however, they are an effective tool to teach artificial insemination (AI) techniques. As an alternative tool, 3D printed cervixes were created using NinjaFlex filament in a Flashforge Creator Pro Dual Extrusion Printer. Designs of cervixes were created with a 3D CAD software while MATLAB was used to generate the random placement of the cervical ring openings to model biological diversity. The objective was to determine the effectiveness of using a 3D printed cervix compared with an excised reproductive tract to teach AI in cattle. Data were collected via surveys for 120 students from 2016 through 2019. Students were divided in two groups prior to entering live animals: 1) Excised reproductive tract and 2) 3D Cervix. The excised reproductive tracts were previously collected, preserved, and confirmed passable by instructors. The 3D cervix group included 3D cervixes mounted in a bovine pelvis inside a box. Overall, 88% of students had success passing the cervix in the cow by the third day. Eighty-seven percent of students thought the excised tracts felt somewhat similar to a live cow, compared with 54% who thought the 3D cervixes alone felt similar to a live cow. When the 3D cervixes were mounted in the box, 17/20 students thought they felt similar to a live cow. For learning AI techniques, 12/21 students thought the excised tracts were more helpful than the 3D cervixes alone (5/21); however, when the 3D cervixes were mounted in the pelvic boxes, 11/21 students preferred this setup compared with the excised tracts (6/21 students). Student feedback suggests the 3D cervixes mounted in pelvic boxes are a useful tool for teaching artificial insemination techniques.
PSV-5 Effect of oxygen administration to piglets at birth on post-natal body temperatures
Authors: Cooper Naomi , Vande Pol Katherine , Ellis Michael , Xiong Yijie , Gates Richard
Published: November 2020

Newborn piglets are susceptible to hypothermia, and associated mortality. Previous research suggested exposing neo-natal piglets to elevated oxygen concentrations reduced post-natal body temperature decline. This study evaluated the effects of oxygen administration at birth on piglet temperatures over 24 h after birth. This study used 296 piglets (24 litters) and was carried out in a standard farrowing facility consisting of crates within pens with a heat lamp. A GRBD was used; sow was the blocking factor; piglet the experimental unit. A replicate was 3 littermates of similar BW; piglets were randomly allotted at birth within replicate to treatment: 1) Control [dried at birth (with a desiccant); returned to farrowing pen]; 2) Ambient Oxygen (dried; placed in a chamber at ambient temperature and oxygen concentration for 20 min); 3) 40% Oxygen (dried; placed in a chamber at ambient temperature and 40% oxygen concentration for 20 min). Piglets were weighed at birth. Rectal temperature was measured at 0, 20, 30, 45, 60, 90, 120, 180, 240 and 1440 min after birth. Data were analyzed using PROC MIXED of SAS. The model included fixed effects of treatment, time of measurement (repeated measure), and the interaction and random effects of block and replicate. There was no treatment effect (P > 0.05) on temperatures at birth and between 120 and 1440 min. Compared to the Control, temperatures were lower (P < 0.05) for the 40% Oxygen treatment between 20 and 90 min and for the Ambient Oxygen treatment between 20 and 45 min. Temperatures at 60 and 90 min for the Ambient Oxygen treatment were intermediate to and not different (P > 0.05) from the other treatments. Results suggest that exposing neo-natal piglets to an oxygen-rich environment in a chamber does not reduce post-natal temperature decline. This research was funded by the National Pork Board.
PSV-2 An exploration of sociality among pre-weaned beef calves
Authors: Robbins Jesse , Parsons Rebecca , Dewell Grant , Haley Derek , Johnson Anna , Dewell Reneé , Millman Suzanne
Published: November 2020

Preferential relationships have been reported between individual cows within a herd. However, less is known about the ontogeny of preferential relationships between calves and subsequent bonding behavior. The objective of this study was to characterize these relationships in pre-weaned beef calves, with the hypothesis that calves form preferential relationships with one another prior to weaning. Ten weeks prior to commencing formal observations, forty mature Angus cows and their calves were randomly selected from a research herd, balanced for calf sex and allocated to one of two grazing paddocks approximately 12 acres in size. Two weeks prior to weaning, direct observations of calf behavior were performed over period of 12 days, using instantaneous scan sampling between 0700-1230h or 1300-1830h, resulting in 6 scans per calf/day X 12 days (72 scans/calf). For each calf, the identity and proximity (in calf lengths) of the calf in closest proximity (aka nearest neighbor) was noted, as well as behavior. The ethogram included calf behavior (grazing, nursing, lying, drinking, locomotion, social contact and standing idle), and sociality was categorized as being within 5 calf lengths of another calf. Sociality was observed in 60% of all scans, and sociality of individual calves ranged from 47% to 72% of the scans observed (72 scans/calf). The statistical model investigating sociality included calf sex and the interaction of day and paddock. Sociality was equally likely in bulls and heifers (0.59 +/- 0.1; p=0.80). A significant day by paddock interaction was also identified (p=0.001), but no obvious trends were apparent. In conclusion, preliminary results provide support for individual differences in sociality of pre-weaned beef calves prior to weaning. Further analysis will explore if preferential relationships exist between individual calves, and how these might be associated with different behavioral states.
PSV-3 Detection of Bruising in Holstein Slaughter Cows with Infrared Thermography
Authors: Kirk Ashlynn , Vogel Kurt
Published: November 2020

Identifying severe bruising in livestock before slaughter is valuable because bruises are likely a source of pain for the animal that cannot be seen by visual inspection. This bruising may result in major trim losses because bruised tissue is considered unfit for human consumption. The objective of this study was to determine if bruising could be detected under the hide of live Holstein cull cows with infrared thermal imaging technology. A FLIR E8 thermal imaging camera was utilized to capture heat emission images pre-hide removal and post hide removal for 36 Holstein cull cows. Images were collected approximately 2 m from each cow and associated carcass images were examined on FLIR Tools software to determine if and where inflammation was detected on the carcass. Trimming occurs throughout the slaughter process, but carcasses may be ‘railed out’ if additional trimming is necessary. Trim loss was collected from railed out carcasses and weighed. The impact of rail-out status on trim loss was significant (P < 0.0001). Carcasses that were railed out (n = 15) yielded 16.2 ± 2.0 kg of trim loss. Carcasses that were not railed out (n = 21) did not experience additional trim loss. Carcasses railed out for additional trimming where inflammation suggested bruising existed (n = 7) yielded 21.0 ± 4.8 kg of trim loss. Carcasses railed out for trimming where thermography did not detect bruising (n = 4) yielded 12.6 ± 6.4 kg of trim loss. The relationship between thermographic detection of bruises and trim loss among railed-out carcasses was not significant (P = 0.3200). Inflammation that predicted rail-out events was 63.64% while carcasses in which rail-out was not detected by thermography was 36.36%. It appears that infrared thermography has potential to identify carcass bruising but additional investigation is warranted to understand its limitations.
PSII-16 Comparative digestibility of polysaccharide-complexed zinc and zinc sulfate in diets for gestating and lactating sows
Authors: Holen Julia P , Johnston Lee , Urriola Pedro E , Garrett Jack E , Shurson Gerald C
Published: November 2020

We hypothesized that digestibility of a zinc polysaccharide complex (PSZn) is greater than zinc sulfate when sows consume high fiber diets containing dried distillers grains with solubles (DDGS). Gilts and sows (n = 32) were blocked according to parity and randomly assigned to one of four dietary treatments. Treatments were: 1) Control (ConZnSO4) – corn-soybean meal based diet + 100 ppm Zn from ZnSO4; 2) Control PSZn (ConPSZn) – corn-soybean meal based diet + 100 ppm Zn from PSZn; 3) DDGS/ZnSO4 – corn-soybean meal-40% DDGS gestation and 30% DDGS lactation diet, each containing 100 ppm Zn from ZnSO4; 4) DDGS/PSZn – corn-soybean meal-40% DDGS gestation and 30% DDGS lactation diet, each containing 100 ppm Zn from PSZn. Titanium dioxide was used as an indigestible marker to evaluate digestibility of nutrients and Zn. Quantitative urine collection was used to calculate retention of Zn in the four dietary treatments. The statistical model included fixed effects of diet, Zn source, and their interaction, and random effects of parity. Endogenous losses of Zn were estimated in sows fed a corn-soybean meal diet without added Zn. There were no differences in Zn concentrations of urine, plasma, colostrum, or milk samples among treatments at any time of the experiment (P > 0.05; data not shown). Gestating sows fed DDGS/PSZn had improved (P < 0.05) ATTD, TTTD, and overall retention of Zn compared to Control treatments (Table 1). Lactating sows consuming diets without DDGS and supplemented PSZn had the greatest (P < 0.05) ATTD, TTTD, and retention of Zn, which were opposite to responses observed in gestation. Furthermore, ATTD, TTTD, and Zn retention for lactating sows consuming DDGS/PSZn were less (P < 0.05) than all other treatments. Zinc digestibility of ZnSO4 and PSZn appears to be differentially influenced by stage of the reproductive cycle and presence of dietary fiber from DDGS.
PSIV-15 Determining the effects of increasing levels of xylanase in nutrient adequate diets on growth performance, carcass characteristics of growing-finishing pigs
Authors: Kerkaert Hayden R , DeRouchey Joel M , Dritz Steve S , Goodband Robert D , Tokach Mike D , Woodworth Jason C , Calderon Cartagena Hilda I , Lahaye Ludovic
Published: November 2020

A total of 1,944 mixed sex growing-finishing pigs (PIC; 337×1050; initial BW of 22.5±0.53 kg) were used in a 131-d growth trial to determine the effects of increasing levels of xylanase in adequate diets on grow-finish pig growth performance and carcass characteristics. The 6 dietary treatments consisted of corn, soybean meal, and dried distillers grains with solubles with added xylanase (Belfeed B 1100 MP; Jefo Nutrition Inc., Saint-Hyacinthe, Quebec) formulated to: 0, 5, 10, 20, 40, and 75 of enzymatic activity for xylanase (IU/kg) with 27 pigs per pen and 12 replicates per treatment. Data were analyzed as a randomized complete block design using lmer function in lme4 package in R with pen considered as the experimental unit, body weight as blocking factor, and treatment as a fixed effect. From d 0 to 70, there was a tendency (quadratic, R=0.068) for average daily gain (ADG) to decrease and then increase with increasing added xylanase, but there was no evidence (R >0.10) of differences for average daily feed intake (ADFI) and feed efficiency (G/F). From d 70 to 131 and overall, there was no evidence of difference (R >0.10) observed for ADG, ADFI, and F/G. There was no evidence for difference (R >0.10) between treatments for number of pigs receiving injectable treatments or mortalities. For carcass traits, increasing xylanase increased then decreased (quadratic, R=0.010) percentage carcass yield. Also, as xylanase increased, percentage lean decreased (linear, R=0.038) and backfat marginally increased (linear, R=0.066). In conclusion, adding increasing levels of xylanase in nutrient adequate diets did not influence growth performance or mortality but did improve carcass yield when intermediate levels were fed.
PSIX-9 Effects of Conditioning Temperature on Pellet Quality of Nursery Pig Diets
Authors: Nichols Gage E , Stark Charles R , Ogles Aundria , Dunmire Kara M , Paulk Chad B
Published: November 2020

The objective of this experiment was to determine the effect of conditioning temperature on pellet durability index (PDI) and pellet hardness. A nursery pig diet was formulated to contain 25% spray-dried whey. Treatments consisted of three different conditioning temperatures: 54, 63, and 71°C. Diets were steam conditioned (245 mm × 1397 mm Wenger twin staff pre-conditioner, Model 150) for approximately 30 sec and pelleted using a 1-ton 30-horsepower pellet mill (1012-2 HD Master Model, California Pellet Mill) with a 4.8 mm × 31.8 mm pellet die (L:D 6.7). The production rate was set at 900 kg/h. Treatments were pelleted at 3 separate time points to provide 3 replicates per treatment. Samples were collected directly after discharging from the pellet mill and cooled in an experimental counterflow cooler. Samples were analyzed for PDI using the Holmen NHP 100 for 60 sec (TekPro Ltd, Norfolk, UK). Pellet hardness was determined by evaluating the peak amount of force applied before the first signs of fracture. Although conditioning temperature was increased in a linear fashion, a quadratic increase (P < 0.002) in hot pellet temperature (HPT) was observed. The HPT were 68, 72, and 74°C for diets conditioned to 54, 63 and 71°C, respectively. Increasing conditioning temperature resulted in increased (linear, P < 0.045) PDI and pellet hardness. As conditioning temperature increased from 54, to 71°C PDI increased from 87% to 92% and the force required to crush pellets increased from 13.5 to 15.9 kg. There was a tendency for a correlation (P < 0.076, r = 0.618, r2 = 0.382) between pellet hardness and PDI. Overall, increasing the conditioning temperature increased pellet hardness and pellet durability.
PSIX-11 Effects of Spray-Dried Lactococcus-Based Fermentation Products on Growth Performance of Nursery Pigs
Authors: Dunmire Kara M , Braun Michaela B , Nichols Gage E , Evans Caitlin E , Saensukjaroenphon Marut , Truelock Courtney N , Woodworth Jason C , Callura Jarred , Paulk Chad B
Published: November 2020

A total of 720 barrows (initially 5.9 kg; Line 200 × 400; DNA, Columbus, NE) were used in a 42-d growth trial to test the effects of spray-dried Lactococcus-based fermentation products on nursery pig performance. There were 24 replications per treatment with pen randomly allotted to 1 of 6 dietary treatments. The positive control (PC) diet included zinc oxide (ZnO; phase 1), ZnO + chlortetracycline (CTC; phase 2) while the negative control (NC) diet did not include ZnO or CTC. Treatment diets included the negative control + 1 of 4 fermentation products (C, D, E, or F) added at 5% of the diet. Phase 3 diets contained a common control diet fed to all pigs plus treatment diets with fermentation products. Data were analyzed with pen as the experimental unit and barn within group as the blocking factor using PROC-GLIMMIX in SAS. From d 0 to 20, there was an overall treatment effect (P = 0.001) where pigs fed PC had increased (P < 0.05) d 20 BW, ADG, ADFI, and improved G:F compared to those fed the NC and NC + fermentation product. Overall, there was a treatment effect (P = 0.03) for pigs fed the PC having improved (P < 0.05) ADG and G:F compared to the NC and NC + fermentation product. In addition, pigs fed the NC had improved (P < 0.05) G:F compared to those fed additive D, E, and F. In conclusion, pigs fed the PC (zinc + CTC) diet had improved performance compared to pigs fed the NC with or without fermentation product. Adding fermentation products to the NC diet did not improve nursery pig performance.