bluuebunny/crossref_metadata_2025 huggingface dataset:
The Immunotherapy Landscape in Adrenocortical Cancer
Authors: Pegna Guillaume J. , Roper Nitin , Kaplan Rosandra N. , Bergsland Emily , Kiseljak-Vassiliades Katja , Habra Mouhammed Amir , Pommier Yves , Del Rivero Jaydira
Published: May 2021

Adrenocortical carcinoma (ACC) is a rare cancer of the adrenal gland that is frequently associated with excess production of adrenal hormones. Although surgical resection may be curative in early-stage disease, few effective therapeutic options exist in the inoperable advanced or metastatic setting. Immunotherapies, inclusive of a broad array of immune-activating and immune-modulating antineoplastic agents, have demonstrated clinical benefit in a wide range of solid and hematologic malignancies. Due to the broad activity across multiple cancer types, there is significant interest in testing these agents in rare tumors, including ACC. Multiple clinical trials evaluating immunotherapies for the treatment of ACC have been conducted, and many more are ongoing or planned. Immunotherapies that have been evaluated in clinical trials for ACC include the immune checkpoint inhibitors pembrolizumab, nivolumab, and avelumab. Other immunotherapies that have been evaluated include the monoclonal antibodies figitumumab and cixutumumab directed against the ACC-expressed insulin-like growth factor 1 (IGF-1) receptor, the recombinant cytotoxin interleukin-13-pseudomonas exotoxin A, and autologous tumor lysate dendritic cell vaccine. These agents have shown modest clinical activity, although nonzero in the case of the immune checkpoint inhibitors. Clinical trials are ongoing to evaluate whether this clinical activity may be augmented through combinations with other immune-acting agents or targeted therapies.
ZNF-281 as the Potential Diagnostic Marker of Oral Squamous Cell Carcinoma
Authors: Starzyńska Anna , Sobocki Bartosz Kamil , Sejda Aleksandra , Sakowicz-Burkiewicz Monika , Szot Olga , Jereczek-Fossa Barbara Alicja
Published: May 2021

ZNF-281 is a zinc finger factor which can lead to cancer progression and metastasis. Its up-regulation reported in many cancers was correlated with metastasis and worsened patients’ prognosis. This is the first study describing ZNF-281 in the context of OSCC. Oral tissue samples drawn from 66 OSCC patients and 36 control patients were collected to determine protein (using immunochemistry and the semi-quantitative H-score method) and mRNA expression levels (using the RT-qPCR reaction). Our aim was to assess the ZNF-281 expression level in OSCC and the control group. Moreover, we determined the impact of ZNF-281 on survival parameters and the association of diversified clinical parameters with ZNF-281 expression. Clinical factors such as grade, AJCC stage and radiotherapy have an impact on the ZNF-281 H-score level, whereas AJCC stage and grade have an influence on ZNF-281 mRNA expression. Our survival analysis indicated that the impact on overall survival is not statistically significant, and the prognostic potential of ZNF-281 is rather limited. Our findings show that both levels of the ZNF-281 H-score and mRNA are decreased in OSCC in comparison to normal tissue. Moreover, we estimated that the H-score can differentiate normal tissue from OSCC with a sensitivity of 97% and specificity of 93.7%.
Short-Term Ex Vivo Culture of CTCs from Advance Breast Cancer Patients: Clinical Implications
Authors: Carmona-Ule Nuria , González-Conde Miriam , Abuín Carmen , Cueva Juan F. , Palacios Patricia , López-López Rafael , Costa Clotilde , Dávila-Ibáñez Ana Belén
Published: May 2021

Background: Circulating tumor cells (CTC) have relevance as prognostic markers in breast cancer. However, the functional properties of CTCs or their molecular characterization have not been well-studied. Experimental models indicate that only a few cells can survive in the circulation and eventually metastasize. Thus, it is essential to identify these surviving cells capable of forming such metastases. Methods: We isolated viable CTCs from 50 peripheral blood samples obtained from 35 patients with advanced metastatic breast cancer using RosetteSepTM for ex vivo culture. The CTCs were seeded and monitored on plates under low adherence conditions and with media supplemented with growth factors and Nanoemulsions. Phenotypic analysis was performed by immunofluorescence and gene expression analysis using RT-PCR and CTCs counting by the Cellsearch® system. Results: We found that in 75% of samples the CTC cultures lasted more than 23 days, predicting a shorter Progression-Free Survival in these patients, independently of having ≥5 CTC by Cellsearch®. We also observed that CTCs before and after culture showed a different gene expression profile. Conclusions: the cultivability of CTCs is a predictive factor. Furthermore, the subset of cells capable of growing ex vivo show stem or mesenchymal features and may represent the CTC population with metastatic potential in vivo.
IR-Surviving NSCLC Cells Exhibit Different Patterns of Molecular and Cellular Reactions Relating to the Multifraction Irradiation Regimen and p53-Family Proteins Expression
Authors: Alhaddad Lina , Pustovalova Margarita , Blokhina Taisia , Chuprov-Netochin Roman , Osipov Andreyan N. , Leonov Sergey
Published: May 2021

Radiotherapy is a primary treatment modality for patients with unresectable non-small cell lung cancer (NSCLC). Tumor heterogeneity still poses the central question of cancer radioresistance, whether the presence of a particular cell population inside a tumor undergoing a selective outgrowth during radio- and chemotherapy give rise to metastasis and tumor recurrence. In this study, we examined the impact of two different multifraction X-ray radiation exposure (MFR) regimens, fraction dose escalation (FDE) in the split course and the conventional hypofractionation (HF), on the phenotypic and molecular signatures of four MFR-surviving NSCLC cell sublines derived from parental A549 (p53 wild-type) and H1299 (p53-null) cells, namely A549FR/A549HR, H1299FR/H1299HR cells. We demonstrate that sublines surviving different MFR regimens in a total dose of 60 Gy significantly diverge in their molecular traits related to irradiation regimen and p53 status. The observed changes regarding radiosensitivity, transformation, proliferation, metabolic activity, partial epithelial-to-mesenchymal transition (EMT) program activation and 1D confined migratory behavior (wound healing). For the first time, we demonstrated that MFR exposure led to the significant decrease in the expression of p63 and p73, the p53-family members, in p53null cells, which correlated with the increase in cell polyploidy. We could not find significant differences in FRA1 expression between parental cells and their sublines that survived after any MFR regimen regardless of p53 status. In our study, the FDE regimen probably causes partial EMT program activation in MFR-survived NSCLC cells through either Vimentin upregulation in p53null or an aberrant N-cadherin upregulation in p53wt cells. The HF regimen likely less influences the EMT activation irrespectively of the p53 status of MFR-survived NSCLC cells. Our data highlight that both MFR regimens caused overall higher cell transformation of p53null H1299FR and H1299HR cells than their parental H1299 cells. Moreover, our results indicate that the FDE regimen raised the radioresistance and transformation of MFR-surviving NSCLC cells irrespectively of their p53 status, though the HF regimen demonstrated a similar effect on p53null NSCLC cells only. Our data once again emphasize that NSCLC therapy approaches should become more personalized according to radiation therapy (RT) regimen, tumor histology, and molecular status of critical proteins.
Translation of c-Met Targeted Image-Guided Surgery Solutions in Oral Cavity Cancer—Initial Proof of Concept Data
Authors: Buckle Tessa , van Alphen Maarten , van Oosterom Matthias N. , van Beurden Florian , Heimburger Nina , van der Wal Jaqueline E. , van den Brekel Michiel , van Leeuwen Fijs W. B. , Karakullukcu Baris
Published: May 2021

Intraoperative tumor identification (extension/margins/metastases) via receptor-specific targeting is one of the ultimate promises of fluorescence-guided surgery. The translation of fluorescent tracers that enable tumor visualization forms a critical component in the realization of this approach. Ex vivo assessment of surgical specimens after topical tracer application could help provide an intermediate step between preclinical evaluation and first-in-human trials. Here, the suitability of the c-Met receptor as a potential surgical target in oral cavity cancer was explored via topical ex vivo application of the fluorescent tracer EMI-137. Freshly excised tumor specimens obtained from ten patients with squamous cell carcinoma of the tongue were incubated with EMI-137 and imaged with a clinical-grade Cy5 prototype fluorescence camera. In-house developed image processing software allowed video-rate assessment of the tumor-to-background ratio (TBR). Fluorescence imaging results were related to standard pathological evaluation and c-MET immunohistochemistry. After incubation with EMI-137, 9/10 tumors were fluorescently illuminated. Immunohistochemistry revealed c-Met expression in all ten specimens. Non-visualization could be linked to a more deeply situated lesion. Tumor assessment was improved via video representation of the TBR (median TBR: 2.5 (range 1.8–3.1)). Ex vivo evaluation of tumor specimens suggests that c-Met is a possible candidate for fluorescence-guided surgery in oral cavity cancer.
A Hybrid Rao-NM Algorithm for Image Template Matching
Authors: Liu Xinran , Wang Zhongju , Wang Long , Huang Chao , Luo Xiong
Published: May 2021

This paper proposes a hybrid Rao-Nelder–Mead (Rao-NM) algorithm for image template matching is proposed. The developed algorithm incorporates the Rao-1 algorithm and NM algorithm serially. Thus, the powerful global search capability of the Rao-1 algorithm and local search capability of NM algorithm is fully exploited. It can quickly and accurately search for the high-quality optimal solution on the basis of ensuring global convergence. The computing time is highly reduced, while the matching accuracy is significantly improved. Four commonly applied optimization problems and three image datasets are employed to assess the performance of the proposed method. Meanwhile, three commonly used algorithms, including generic Rao-1 algorithm, particle swarm optimization (PSO), genetic algorithm (GA), are considered as benchmarking algorithms. The experiment results demonstrate that the proposed method is effective and efficient in solving image matching problems.
Normalized Multivariate Time Series Causality Analysis and Causal Graph Reconstruction
Authors: Liang X. San
Published: May 2021

Causality analysis is an important problem lying at the heart of science, and is of particular importance in data science and machine learning. An endeavor during the past 16 years viewing causality as a real physical notion so as to formulate it from first principles, however, seems to have gone unnoticed. This study introduces to the community this line of work, with a long-due generalization of the information flow-based bivariate time series causal inference to multivariate series, based on the recent advance in theoretical development. The resulting formula is transparent, and can be implemented as a computationally very efficient algorithm for application. It can be normalized and tested for statistical significance. Different from the previous work along this line where only information flows are estimated, here an algorithm is also implemented to quantify the influence of a unit to itself. While this forms a challenge in some causal inferences, here it comes naturally, and hence the identification of self-loops in a causal graph is fulfilled automatically as the causalities along edges are inferred. To demonstrate the power of the approach, presented here are two applications in extreme situations. The first is a network of multivariate processes buried in heavy noises (with the noise-to-signal ratio exceeding 100), and the second a network with nearly synchronized chaotic oscillators. In both graphs, confounding processes exist. While it seems to be a challenge to reconstruct from given series these causal graphs, an easy application of the algorithm immediately reveals the desideratum. Particularly, the confounding processes have been accurately differentiated. Considering the surge of interest in the community, this study is very timely.
Overlapping Community Detection Based on Attribute Augmented Graph
Authors: Lin Hanyang , Zhan Yongzhao , Zhao Zizheng , Chen Yuzhong , Dong Chen
Published: May 2021

There is a wealth of information in real-world social networks. In addition to the topology information, the vertices or edges of a social network often have attributes, with many of the overlapping vertices belonging to several communities simultaneously. It is challenging to fully utilize the additional attribute information to detect overlapping communities. In this paper, we first propose an overlapping community detection algorithm based on an augmented attribute graph. An improved weight adjustment strategy for attributes is embedded in the algorithm to help detect overlapping communities more accurately. Second, we enhance the algorithm to automatically determine the number of communities by a node-density-based fuzzy k-medoids process. Extensive experiments on both synthetic and real-world datasets demonstrate that the proposed algorithms can effectively detect overlapping communities with fewer parameters compared to the baseline methods.
Recent Advances on Materials for Lithium-Ion Batteries
Authors: Barbosa João C. , Gonçalves Renato , Costa Carlos M. , Lanceros-Mendez Senentxu
Published: May 2021

Environmental issues related to energy consumption are mainly associated with the strong dependence on fossil fuels. To solve these issues, renewable energy sources systems have been developed as well as advanced energy storage systems. Batteries are the main storage system related to mobility, and they are applied in devices such as laptops, cell phones, and electric vehicles. Lithium-ion batteries (LIBs) are the most used battery system based on their high specific capacity, long cycle life, and no memory effects. This rapidly evolving field urges for a systematic comparative compilation of the most recent developments on battery technology in order to keep up with the growing number of materials, strategies, and battery performance data, allowing the design of future developments in the field. Thus, this review focuses on the different materials recently developed for the different battery components—anode, cathode, and separator/electrolyte—in order to further improve LIB systems. Moreover, solid polymer electrolytes (SPE) for LIBs are also highlighted. Together with the study of new advanced materials, materials modification by doping or synthesis, the combination of different materials, fillers addition, size manipulation, or the use of high ionic conductor materials are also presented as effective methods to enhance the electrochemical properties of LIBs. Finally, it is also shown that the development of advanced materials is not only focused on improving efficiency but also on the application of more environmentally friendly materials.
Economic and Social Aspects of Using Energy from PV and Solar Installations in Farmers’ Households in the Podkarpackie Region
Authors: Kata Ryszard , Cyran Kazimierz , Dybka Sławomir , Lechwar Małgorzata , Pitera Rafał
Published: May 2021

The growing energy needs of agriculture, the need to reduce the burning of fossil fuels, and, on the other hand, the increasing technical efficiency are contributing to the wider use of solar energy technology in agriculture. The aim of the article is to identify factors determining farmers’ investments in solar photovoltaic and solar thermal installations for electricity and heat production, to establish the proportion between the consumption of such energy for the needs of the farmer’s family and for the needs of the farm, and to identify the drivers of solar energy use in agricultural production. Empirical materials were collected through surveys of farmers conducted at the end of 2020 in south-eastern Poland, in the Podkarpackie region. It is a region characterized by significant land fragmentation. Producing energy from renewable sources can be an opportunity for farmers not only to reduce household expenses, but also to increase agricultural income. As a result, it can be a driver of sustainable agricultural development in the region. The article presents the most important economic and social determinants that stimulate the adoption of solar photovoltaic and solar thermal technologies by farmers for the needs of their households as well as for agricultural production.
Economic Development, CO2 Emissions and Energy Use Nexus-Evidence from the Danube Region Countries
Authors: Litavcová Eva , Chovancová Jana
Published: May 2021

The aim of this study is to examine the empirical cointegration, long-run and short-run dynamics and causal relationships between carbon emissions, energy consumption and economic growth in 14 Danube region countries over the period of 1990–2019. The autoregressive distributed lag (ARDL) bounds testing methodology was applied for each of the examined variables as a dependent variable. Limited by the length of the time series, we excluded two countries from the analysis and obtained valid results for the others for 26 of 36 ARDL models. The ARDL bounds reliably confirmed long-run cointegration between carbon emissions, energy consumption and economic growth in Austria, Czechia, Slovakia, and Slovenia. Economic growth and energy consumption have a significant impact on carbon emissions in the long-run in all of these four countries; in the short-run, the impact of economic growth is significant in Austria. Likewise, when examining cointegration between energy consumption, carbon emissions, and economic growth in the short-run, a significant contribution of CO2 emissions on energy consumptions for seven countries was found as a result of nine valid models. The results contribute to the information base essential for making responsible and informed decisions by policymakers and other stakeholders in individual countries. Moreover, they can serve as a platform for mutual cooperation and cohesion among countries in this region.
Investments in Renewable Energy Sources in Basic Units of Local Government in Rural Areas
Authors: Klepacki Bogdan , Kusto Barbara , Bórawski Piotr , Bełdycka-Bórawska Aneta , Michalski Konrad , Perkowska Aleksandra , Rokicki Tomasz
Published: May 2021

The main purpose of the study was to identify the level and factors influencing investments in renewable energy sources (RES) in basic local government units in rural areas. The specific objectives were to define the conditions for the development of renewable energy sources in Poland, to determine the directions of changes as well as the importance of renewable energy in Poland, to present the relationship between the level of expenditure on renewable energy and budget components in rural and rural-urban communes. The Świętokrzyskie voivodeship (Voivodship—a unit of the highest administration level in Poland, since 1990 a unit of the primary territorial division of government administration, since 1999 also a unit of local government, there were 16 voivodships in Poland), which is one of the centrally located voivodeships in Poland, was purposefully selected for the research. The research period covered the years 2016–2019. The sources of materials were the literature on the subject, as well as empirical materials obtained at the Voivodeship Statistical Office. The following methods were used for the analysis and presentation of materials: descriptive, tabular, graphical, Gini concentration coefficient, Lorenz concentration curve, Kendall’s tau correlation coefficient and Spearman’s rank correlation coefficient. Poland is one of the countries with quite high dependence on hard and brown coal. Changes in the structure of energy sources are slow. Investments in renewable energy are necessary. The problem in this respect is the lack of a proper law. Despite this, investments in renewable energy are being made in rural areas. In the Świętokrzyskie voivodeship, only 28% of communes made such investments. It was found that only in urban rural communes the amount of investment expenditures in renewable energy sources was related to the level of budget expenditures and property expenditures of the commune. The amount of support from the European Union aid funds was positively correlated with the level of expenditure on investments in renewable energy. Therefore, it can be concluded that without the support from EU funds, it is not possible to invest in renewable energy in local government units.
Adaptive Mode Selection Strategy for Series-Parallel Hybrid Electric Vehicles Based on Variable Power Reserve
Authors: Fan Jingzheng , Zu Bingfeng , Zhou Jianwei , Wang Zhen , Wang Haopeng
Published: May 2021

When the series-parallel hybrid electric vehicle exits the pure electric mode, the battery provides power for the drive motor and integrated starter generator (ISG) to drive the vehicle and start the engine. If the battery discharge power is insufficient, the driving power will drop, which will inhibit the vehicle from accelerating and impair drivability. Considering that the mode selection strategy determines the timing of mode switching, this paper proposes an adaptive mode selection strategy based on variable power reserve to allow the vehicle to switch mode considering the battery power limitation. The effectiveness of this strategy is verified by simulation, and its influence on fuel consumption and battery utilization is analyzed. Compared with the mode selection strategy based on logic thresholds at the same initial battery state of charge (SOC), under the high-speed and aggressive US06 cycle, the total driving power drop is reduced by 74.2%, and the over-discharge power of the battery is fully restrained while keep almost the same fuel consumption; under the city FTP cycle, the total driving power drop is reduced 65%, and fuel consumption is reduced while maintaining SOC at a reasonable level.
Study on Worldwide Embodied Impacts of Construction: Analysis of WIOD Release 2016
Authors: Mishina Yu , Sasaki Yosuke , Yokoyama Keizo
Published: May 2021

Net-zero-energy buildings (ZEBs) that contribute to making annual energy consumption balances zero are effective measures for reducing greenhouse gas (GHG) emissions in the construction sector. As the application of ZEBs progresses, GHG emissions during the construction of buildings and the manufacturing of materials and products (called construction EG) account for a relatively large proportion of overall emissions. This study aimed to clarify construction EG as a means by which to formulate policies for the reduction of emissions in each country. The construction EGs of 43 countries from 2011 were analyzed. The 56-sector input/output table and CO2 emission data of the 2016 World Input/Output Database, published by the EU, were both used in this analysis. It was found that the construction sector accounted for the highest proportion of total CO2 emissions. Moreover, the fraction of construction EG tended to be higher in developing countries such as China and India, while developed countries tended to contribute a lower fraction of construction EG. Construction EGs were shown to be heavily influenced by the sectors that manufacture “cement”, “steel bars and steel frames”, and “energy sources”. Thus, it is very important to advance technological developments to reduce CO2 emissions within these sectors. The annual variation of construction EGs and CO2 emissions from 2000 to 2014 showed that the construction EGs and total CO2 emissions in developing countries were increasing, whereas emissions from developed countries have been decreasing slightly.
Improving the Development Technology of an Oil and Gas Company Using the Minimax Optimality Criterion
Authors: Borodin Alex , Tvaronavičienė Manuela , Vygodchikova Irina , Kulikov Andrey , Skuratova Marina , Shchegolevatykh Natalia
Published: May 2021

The article deals with the problem of adaptation of the Russian oil and gas company (Novatek, Russia) to the rapidly changing external environment, the avalanche of data from competitors, and the need to filter important information for business development and the prosperity of the industry as a whole. The approach is based on the system of integrated software monitoring of key business processes at the enterprise developed by the authors—from the formation of the idea of a new product to its implementation to paying customers. The scientific novelty lies in the use of an optimization model that allows for minimizing the maximum losses of the investor at all levels of decision-making, from the distribution of capital between companies, to the optimization of internal reserves to increase the competitiveness of the company. The toolkit is a minimax model that allows you to redistribute the shares of investor influence at the portfolio level, and then within the business processes of each company selected by investors, in order to achieve the optimal solution in accordance with the selected estimated indicators. Application of the well-known portfolio investment models of Markowitz, Tobin, Sharp, etc. is not possible due to the lack of necessary data on the basis of which the probabilistic parameters involved in the model are estimated. Even if we get them, it is necessary to take into account the level of correlation influence of the technological process in the composition of each subsystem, which is unacceptable for the data used, as it leads to a strong increase in errors. Using minimax and a systematic approach allows you to minimize such errors by choosing a balanced concentration of distributed assets for both the investor and the buyer. To this end, a three-way analysis of the company’s development was carried out and a technology for comprehensive improvement of the company’s activities was developed in the following areas: the company’s rating in the industry, financial condition, and interaction with counterparties using merchandising technologies. Tools for optimal image zoning at the Novatek site using the minimax approximation criterion have been developed. The technology provides a procedure for creating a comfortable mode of image perception based on high-tech visualization of merchandising, zoning of the screen area, and a mathematical approach that allows you to develop a calculation algorithm.
Edible Far Eastern Ferns as a Dietary Source of Long-Chain Polyunsaturated Fatty Acids
Authors: Nekrasov Eduard V. , Svetashev Vasily I.
Published: May 2021

Young fronds of ferns are consumed as a vegetable in many countries. The aim of this study was to analyze three fern species that are available for sale in the Russian Far East as dietary sources in terms of fatty acids that are important for human physiology: arachidonic acid (20:4n-6, ARA), eicosapentaenoic acid (20:5n-3, EPA) and other valuable long-chain polyunsaturated fatty acids. The content of ARA and EPA was 5.5 and 0.5 mg/g dry weight, respectively, in Pteridium aquilinum, 4.1 and 1.1 in Matteuccia struthiopteris, and 2.2 and 0.8 in Osmundastrum asiaticum. Salted fronds of P. aquilinum contained less these fatty acids than the raw fronds, with a decrease of up to 49% for ARA and 65% for EPA. These losses were less pronounced or even insignificant in dried fronds. Cooked ferns preserved significant portions of the long-chain polyunsaturated fatty acids: cooked P. aquilinum contained 4.4 mg/g dry weight ARA and 0.3 mg/g dry weight EPA. The ferns may provide a supplemental dietary source of these valuable long-chain polyunsaturated fatty acids, especially for vegetarian diets.
Influence of Muscle Type on Physicochemical Parameters, Lipolysis, Proteolysis, and Volatile Compounds throughout the Processing of Smoked Dry-Cured Ham
Authors: Marušić Radovčić Nives , Poljanec Ivna , Petričević Sandra , Mora Leticia , Medić Helga
Published: May 2021

The influence of muscle type (biceps femoris, BF and semimembranosus, SM) on physicochemical parameters, volatile compounds, and the extent of proteolysis and lipolysis during the manufacturing of smoked dry-cured ham was investigated. A total of fifty smoked hams were sampled: raw ham, after salting, smoking, drying, and ripening. Almost all physicochemical parameters were affected by muscle type, manufacturing stage and their interactions. SM had lower water, ash, NaCl content, and water activity (aw), while fat and protein content were higher after ripening compared to BF. BF showed higher L*a*b* values compared to SM. The results of texture profile analysis showed that almost all analyzed parameters were influenced by muscle type and production stage. A total of 88 volatile compounds were identified, showing an increase in its number during processing: 31 volatile compounds were identified in raw ham and 72 after the ripening phase. Aldehydes and phenols were the predominant groups of compounds, followed by alcohols, ketones, aromatic hydrocarbons, aliphatic hydrocarbons, esters, and terpenes. Muscle type and production phase significantly affected lipid oxidation and the index of proteolysis: in SM, thiobarbituric acid reactive substances (TBARS) increased faster than in BF, while proteolysis had an opposite effect and was more pronounced in BF.
Effects of Tea against Alcoholic Fatty Liver Disease by Modulating Gut Microbiota in Chronic Alcohol-Exposed Mice
Authors: Li Bangyan , Mao Qianqian , Zhou Dandan , Luo Min , Gan Renyou , Li Hangyu , Huang Siyu , Saimaiti Adila , Shang Ao , Li Huabin
Published: May 2021

Gut microbiota dysbiosis has been a crucial contributor to the pathogenesis of alcoholic fatty liver disease (AFLD). Tea is a popular beverage worldwide and exerts antioxidant and anti-inflammatory activities, as well as hepatoprotective effects. However, the potential role of gut microbiota regulated by tea in the prevention and management of AFLD remains unclear. Here, the protective effects of oolong tea, black tea, and dark tea on AFLD and its regulation of gut microbiota in chronic alcohol-exposed mice were explored and investigated. The results revealed that tea supplementation significantly prevented liver steatosis, decreased oxidative stress and inflammation, and modulated gut microbiota in chronic alcohol-exposed mice, especially oolong tea and dark tea. However, black tea showed less effectiveness against liver injury caused by alcohol. Moreover, the diversity, structure and composition of chronic alcohol-disrupted gut microbiota were restored by the supplementation of oolong tea and dark tea based on the analysis of gut microbiota. Furthermore, the relationship between liver injury biochemical indicators and gut microbiota indicated that some specific bacteria, such as Bacteroides, Alloprevotella, and Parabacteroides were closely associated with AFLD. In addition, the phytochemical components in tea extracts were measured by high-performance liquid chromatography, which could contribute to preventive effects on AFLD. In summary, oolong tea and dark tea could prevent chronic alcohol exposure-induced AFLD by modulating gut microbiota.
Face Mask Usage among Young Polish People during the COVID-19 Epidemic—An Evolving Scenario
Authors: Reszke Radomir , Szepietowska Marta , Krajewski Piotr K. , Matusiak Łukasz , Białynicki-Birula Rafał , Szepietowski Jacek C.
Published: May 2021

The usage of face masks has been mandated in many countries in an attempt to diminish the spread of SARS-CoV-2. In this cross-sectional study, we aimed to determine face mask-wearing behaviors and practices in 1173 young Polish people during the second wave of the COVID-19 epidemic in October 2020. The majority of respondents (97.4%) declared that they wore face masks in areas/situations where it is mandatory. The most common types of utilized face masks were cloth masks (47.7%) and surgical masks (47%), followed by respirators (N95/FFP3) (3.2%) and half-face elastomeric respirators (0.9%). Over 38% reported frequently disinfecting their face masks, especially females. Respondents reporting personal atopic predisposition (64.5% vs. 72.1%; p = 0.02) or sensitive skin (65.5% vs. 74.3%; p = 0.005) declared multiple use of face masks less commonly than other individuals. Individuals suffering from facial skin lesions declared disinfecting face masks more commonly (40.8% vs. 34.9%; p = 0.04). Overall, the self-declared utilization of face masks among young people in Poland has improved since the beginning of the epidemic as compared with our previous study. Until the mass vaccination of the public is achieved and government policy is changed, face mask use remains a valuable tool to decrease the transmission of SARS-CoV-2.
Scheduling of Anaesthesia Operations in Operating Rooms
Authors: Hsu Pi-Yu , Lo Shao-Hua , Hwang Hsin-Gin , Lin Bertrand M. T.
Published: May 2021

This paper considers scheduling of surgical operations across multiple operating rooms subject to the limited availability of anaesthetists. The objective is to construct a feasible operations schedule that has the minimum makespan, i.e., the completion time of all operations. We abstract the problem into a theoretical server scheduling problem and formulate it in a mathematical form by proposing an integer programming model. Due to the intractability of its computing time, we circumvent the exact approaches and develop two approximation methods. Then, the steepest descent search is adopted for improving the solutions. Computational study suggests that the proposed methods can produce quality solutions in a few seconds.
Coir, an Alternative to Peat—Effects on Plant Growth, Phytochemical Accumulation, and Antioxidant Power of Spinach
Authors: Machado Rui M. A. , Alves-Pereira Isabel , Ferreira Rui , Gruda Nazim S.
Published: May 2021

The effects of four commercial substrates, a peat-based substrate, and three coir types (coir pith, coir chips, and coir pith + fibers) on yield, phytochemical accumulation, and antioxidant activity were evaluated in Spinacia oleracea L. cv. ‘Manatee’. Soil-blocked spinach seedlings were transplanted into Styrofoam planting boxes filled with the substrate. Each planting box was irrigated daily by drip with a complete nutrient solution, and the irrigation scheduling was optimized to the peat. Leaf area and fresh yield in coir pith and coir pith + fiber were similar to those obtained in peat. However, shoot dry weight accumulation and leaf chlorophyll were lower in plants grown in coir. Substrate type did not affect leaf carotenoids. Total flavonoid content was higher in plants grown in the different types of coir. Total phenols and antioxidant activity (DPPH) were higher in plants grown in coir pith. This indicates that the different coir types, mainly coir pith, may provide an alternative to peat since they allowed a high fresh yield to be reached and the total flavonoids to be increased. In contrast, the levels of other phytochemicals and antioxidant activity were usual for spinach. However, further research is necessary to analyze the effects of irrigation scheduling and the nutrient solution adjusted to each growing medium on yield and phytochemical accumulation.
Recovery-Stress Response of Blood-Based Biomarkers
Authors: Hacker Sebastian , Reichel Thomas , Hecksteden Anne , Weyh Christopher , Gebhardt Kristina , Pfeiffer Mark , Ferrauti Alexander , Kellmann Michael , Meyer Tim , Krüger Karsten
Published: May 2021

The purpose of this study was to investigate blood-based biomarkers and their regulation with regard to different recovery-stress states. A total of 35 male elite athletes (13 badminton, 22 soccer players) were recruited, and two venous blood samples were taken: one in a ‘recovered’ state (REC) after a minimum of one-day rest from exercise and another one in a ‘non-recovered’ state (NOR) after a habitual loading microcycle. Overall, 23 blood-based biomarkers of different physiologic domains, which address inflammation, muscle damage, and tissue repair, were analyzed by Luminex assays. Across all athletes, only creatine kinase (CK), interleukin (IL-) 6, and IL-17A showed higher concentrations at NOR compared to REC time points. In badminton players, higher levels of CK and IL-17A at NOR were found. In contrast, a higher value for S100 calcium-binding protein A8 (S100A8) at REC was found in badminton players. Similar differences were found for BDNF in soccer players. Soccer players also showed increased levels of CK, and IL-6 at NOR compared to REC state. Several molecular markers were shown to be responsive to differing recovery-stress states, but their suitability as biomarkers in training must be further validated.
Association of Health Insurance Status with Outcomes of Sepsis in Adult Patients: A Retrospective Cohort Study
Authors: Wang Gaon-Sorae , You Kyoung-Min , Jo You-Hwan , Lee Hui-Jai , Shin Jong-Hwan , Jung Yoon-Sun , Hwang Ji-Eun
Published: May 2021

(1) Background: Sepsis is a life-threatening disease, and various demographic and socioeconomic factors affect outcomes in sepsis. However, little is known regarding the potential association between health insurance status and outcomes of sepsis in Korea. We evaluated the association of health insurance and clinical outcomes in patients with sepsis. (2) Methods: Prospective cohort data of adult patients with sepsis and septic shock from March 2016 to December 2018 in three hospitals were retrospectively analyzed. We categorized patients into two groups according to their health insurance status: National Health Insurance (NHI) and Medical Aid (MA). The primary end point was in-hospital mortality. The multivariate logistic regression model and propensity score matching were used. (3) Results: Of a total of 2526 eligible patients, 2329 (92.2%) were covered by NHI, and 197 (7.8%) were covered by MA. The MA group had fewer males, more chronic kidney disease, more multiple sources of infection, and more patients with initial lactate > 2 mmol/L. In-hospital, 28-day, and 90-day mortality were not significantly different between the two groups and in-hospital mortality was not different in the subgroup analysis. Furthermore, health insurance status was not independently associated with in-hospital mortality in multivariate analysis and was not associated with survival outcomes in the propensity score-matched cohort. (4) Conclusions: Our propensity score-matched cohort analysis demonstrated that there was no significant difference in in-hospital mortality by health insurance status in patients with sepsis.
Process Evaluation of the SImplification of Medications Prescribed to Long-tErm Care Residents (SIMPLER) Cluster Randomized Controlled Trial: A Mixed Methods Study
Authors: Sluggett Janet K. , Hughes Georgina A. , Ooi Choon Ean , Chen Esa Y. H. , Corlis Megan , Hogan Michelle E. , Caporale Tessa , Van Emden Jan , Bell J. Simon
Published: May 2021

Complex medication regimens are highly prevalent, burdensome for residents and staff, and associated with poor health outcomes in residential aged care facilities (RACFs). The SIMPLER study was a non-blinded, matched-pair, cluster randomized controlled trial in eight Australian RACFs that investigated the one-off application of a structured 5-step implicit process to simplify medication regimens. The aim of this study was to explore the processes underpinning study implementation and uptake of the medication simplification intervention. A mixed methods process evaluation with an explanatory design was undertaken in parallel with the main outcome evaluation of the SIMPLER study and was guided by an established 8-domain framework. The qualitative component included a document analysis and semi-structured interviews with 25 stakeholders (residents, family, research nurses, pharmacists, RACF staff, and a general medical practitioner). Interviews were transcribed verbatim and reflexively thematically content analyzed. Descriptive statistics were used to summarize quantitative data extracted from key research documents. The SIMPLER recruitment rates at the eight RACFs ranged from 18.9% to 48.6% of eligible residents (38.4% overall). Participation decisions were influenced by altruism, opinions of trusted persons, willingness to change a medication regimen, and third-party hesitation regarding potential resident distress. Intervention delivery was generally consistent with the study protocol. Stakeholders perceived regimen simplification was beneficial and low risk if the simplification recommendations were individualized. Implementation of the simplification recommendations varied between the four intervention RACFs, with simplification implemented at 4-month follow-up for between 25% and 86% of residents for whom simplification was possible. Good working relationships between stakeholders and new remunerated models of medication management were perceived facilitators to wider implementation. In conclusion, the one-off implicit medication simplification intervention was feasible and generally delivered according to the protocol to a representative sample of residents. Despite variable implementation, recommendations to simplify complex regimens were valued by stakeholders, who also supported wider implementation of medication simplification in RACFs.
The Safety Assessment of Toxic Metals in Commonly Used Herbs, Spices, Tea, and Coffee in Poland
Authors: Kowalska Grażyna
Published: May 2021

The presented study was aimed at the determination of the level of contamination with heavy metals (Cd, Pb, As, and Hg) in 240 samples of plant materials, i.e., herbal raw materials, spices, tea, and coffee. Moreover, a probabilistic risk assessment (noncarcinogenic and carcinogenic risks) was estimated by models including target hazard quotient (THQ) and cancer risk (CR). The samples were subjected to microwave mineralisation with the use of HNO3 (65%), while the determination of the content of the elements was performed with the use of inductively coupled plasma mass spectrometer (ICP–MS) and a mercury analyser. The element which was characterised by the highest level of accumulation in the analysed samples was lead (from 0.010 to 5.680 mg/kg). Among the heavy metals under analysis, the lowest concentration was noted in the case of mercury (from 0.005 to 0.030 mg/kg). A notably higher level of contamination with heavy metals was noted in the analysed samples of herbs and spices (0.005–5.680 mg/kg), compared to samples of tea and coffee (0.005–0.791 mg/kg). According to the guidelines of the World Health Organisation (WHO) concerning the limits of contamination of samples of herbal raw materials with heavy metals, lead levels exceeding the limits were only noted in 24 samples of herbs (18%). In all of the analysed samples of spices, tea, and coffee, no instances of exceeded limits were noted for any of the analysed heavy metals. The values of TTHQmax (in relation to the consumption of the analysed products) were as follows: up to 4.23 × 10−2 for spices, up to 2.51 × 10−1 for herbs, up to 4.03 × 10−2 for China tea, and up to 1.25 × 10−1 for roasted coffee beans. As the value of THQ ≤1, there is no probability of the appearance of undesirable effects related to the consumption of the analysed group of raw materials and products of plant origin. The CR value for As (max. value) was 1.29 × 10−5, which is lower than the maximum acceptable level of 1 × 10−4 suggested by United States Environmental Protection Agency (USEPA).
Erratum: Okui, T. Socioeconomic Disparities in All-Cause and Cause-Specific Mortality Rates among Municipalities in Japan, 1999–2019. Int. J. Environ. Res. Public Health 2020, 17, 9213
Authors: Okui Tasuku
Published: May 2021

The author wants to correct the values of the 95% confidence interval of the standardized mortality ratio (SMR) in Table 3 and Table 4 in the following paper [...]
Participation in Play and Leisure Activities of Young Children with Autism Spectrum Disorder and Typically Developing Children in Taiwan: A Preliminary Study
Authors: Huang Chi-Ching , Kang Lin-Ju
Published: May 2021

Participation in enjoyable activities is essential for the health and development of young children with and without disabilities. For preschool children with autism spectrum disorder (ASD), there is limited knowledge regarding their participation in play, learning, recreation, and social activities. This was a preliminary study that compares the participation between children 2–6 years of age with ASD (n = 25) and age- and sex-matched typically developing (TD) (n = 25) children. The Chinese version of the Assessment of Preschool Children’s Participation (APCP-C) measures participation in play, skill development, active physical recreation, and social activities. Parents of the children in this study completed the APCP-C by structured interview. The results showed that children with ASD had lower participation diversity and intensity than TD children in play activities. A lower percentage of children participating in individual activity was found for children with ASD than TD children in most APCP-C activities. Professionals that serve young children with special needs are encouraged to partner with parents to provide playful and socially enhancing activities for preschool children with ASD.
Emerging Technologies for Degradation of Dichlorvos: A Review
Authors: Zhang Yuming , Zhang Wenping , Li Jiayi , Pang Shimei , Mishra Sandhya , Bhatt Pankaj , Zeng Daxing , Chen Shaohua
Published: May 2021

Dichlorvos (O,O-dimethyl O-(2,2-dichlorovinyl)phosphate, DDVP) is a widely acknowledged broad-spectrum organophosphorus insecticide and acaracide. This pesticide has been used for more than four decades and is still in strong demand in many developing countries. Extensive application of DDVP in agriculture has caused severe hazardous impacts on living systems. The International Agency for Research on Cancer of the World Health Organization considered DDVP among the list of 2B carcinogens, which means a certain extent of cancer risk. Hence, removing DDVP from the environment has attracted worldwide attention. Many studies have tested the removal of DDVP using different kinds of physicochemical methods including gas phase surface discharge plasma, physical adsorption, hydrodynamic cavitation, and nanoparticles. Compared to physicochemical methods, microbial degradation is regarded as an environmentally friendly approach to solve several environmental issues caused by pesticides. Till now, several DDVP-degrading microbes have been isolated and reported, including but not limited to Cunninghamella, Fusarium, Talaromyces, Aspergillus, Penicillium, Ochrobium, Pseudomonas, Bacillus, and Trichoderma. Moreover, the possible degradation pathways of DDVP and the transformation of several metabolites have been fully explored. In addition, there are a few studies on DDVP-degrading enzymes and the corresponding genes in microorganisms. However, further research relevant to molecular biology and genetics are still needed to explore the bioremediation of DDVP. This review summarizes the latest development in DDVP degradation and provides reasonable and scientific advice for pesticide removal in contaminated environments.