bluuebunny/crossref_metadata_2025 huggingface dataset:
Paraoxonase single nucleotide variants show associations with polycystic ovary syndrome: a meta-analysis
Authors: Kunjantarachot Anthicha , Pabalan Noel , Jarjanazi Hamdi , Christofolini Denise Maria , Montagna Erik , Barbosa Caio Parente , Bianco Bianca
Published: November 2020

Background

Etiology of polycystic ovary syndrome (PCOS) is attributed to genetic and environmental factors. One environmental factor is oxidative stress. Paraoxonase 1 (PON1) is an antioxidant high-density lipoprotein-associated enzyme encoded by thePON1gene. ThePON1gene has been implicated in the risk for PCOS, the influence of which appears to come from single nucleotide variants (SNVs) at multiple genetic loci. However, association study reports have been inconsistent which compels a meta-analysis to obtain more precise estimates.

Methods

From 12 publications, extracted genotype data were used in two genetic procedures. First, linkage disequilibrium (LD) was used to group eightPONSNVs into three: LD1, LD2 and LD3. Second, frequencies of the variant (var), wild-type (wt) and heterozygous (het) genotypes were used for genetic modeling (allele-genotype for LD1 and standard for LD2 and LD3). Risk associations were expressed in terms of pooled odds ratios (ORs), 95% confidence intervals (CIs) andPa-values. Evidence was considered strong when significance was high (Pa < 0.0001) and heterogeneity absent (I2 = 0%). Pooled effects were subjected to modifier (power), subgroup (Asian/Caucasian), outlier, sensitivity and publication bias treatments. Multiple comparisons were Bonferroni-corrected.

Results

This meta-analysis generated 11 significant outcomes, five in LD1, six in LD2 and none in LD3. All six LD2 outcomes did not survive the Bonferroni-correction but two of the five in LD1 did. These two core LD1 findings conferred greater odds of PCOS to thevarallele in the highly significant (Pa < 0.0001) overall (OR 1.44, 95% CI 1.24–1.67) and Asian (OR 1.41, 95% CI 1.20–1.65) outcomes. Of these two core outcomes, the Asian effect was homogeneous (I2 = 0%) but not the overall (I2 = 29%).

Conclusions

Of the eightPONSNVs examined, two (rs854560 and rs662) were associated with PCOS risk. These 1.4-fold increased risk effects rendered Asians susceptible to PCOS. High statistical power, high significance, zero to low-level heterogeneity, robustness and lack of bias in the core outcomes underpinned the strong evidence for association.
The dark side of the spoon - glucose, ketones and COVID-19: a possible role for ketogenic diet?
Authors: Paoli Antonio , Gorini Stefania , Caprio Massimiliano
Published: November 2020

The novel coronavirus disease (COVID-19) is posing a serious challenge to the health-care systems worldwide, with an enormous impact on health conditions and loss of lives. Notably, obesity and its related comorbidities are strictly related with worse clinical outcomes of COVID-19 disease. Recently, there is a growing interest in the clinical use of ketogenic diets (KDs), particularly in the context of severe obesity with related metabolic complications. KDs have been proven effective for a rapid reduction of fat mass, preserving lean mass and providing an adequate nutritional status. In particular, the physiological increase in plasma levels of ketone bodies exerts important anti-inflammatory and immunomodulating effects, which may reveal as precious tools to prevent infection and potential adverse outcomes of COVID-19 disease. We discuss here the importance of KDs for a rapid reduction of several critical risk factors for COVID-19, such as obesity, type 2 diabetes and hypertension, based on the known effects of ketone bodies on inflammation, immunity, metabolic profile and cardiovascular function. We do believe that a rapid reduction of all modifiable risk factors, especially obesity with its metabolic complications, should be a pillar of public health policies and interventions, in view of future waves of SARS-CoV-2 infection.
Emergence of plasmid-mediated mcr genes from Gram-negative bacteria at the human-animal interface
Authors: Javed Humera , Saleem Sidrah , Zafar Aizza , Ghafoor Aamir , Shahzad Ahmad Bin , Ejaz Hasan , Junaid Kashaf , Jahan Shah
Published: November 2020

Background

The global emergence of plasmid-mediated colistin resistance (Col-R) conferred by mcr genes in gram-negative rods (GNRs) has jeopardized the last treatment option for multidrug-resistant bacterial infections in humans. This study aimed to assess the emergence of mcr gene-mediated Col-R in GNRs isolated from humans and animals in Pakistan.

Methods

Animal and clinical specimens collected from various sources were prospectively analysed using standard microbiological procedures. Pathogens were identified using the API 20E and API 20NE systems (bioMerieux). Minimum inhibitory concentration (MIC) against colistin was determined using the MIC detection methods, and multiplex polymerase chain reaction (PCR) was used to amplify the mcr-1 to mcr-5 genes.

Results

We isolated 126 (88.1%) animal and 17 (11.9%) human Col-R phenotypes, among which there was a significant association (P < 0.01) of Escherichia coli and Proteus mirabilis with animals and of Acinetobacter baumannii with humans. Animal strains exhibited statistically significant (P < 0.05) resistance to co-trimoxazole, chloramphenicol, and moxifloxacin, and the human pathogens exhibited statistically significant (P < 0.05) antibiotic resistance to cephalosporins, carbapenems, and piperacillin-tazobactam. For Col-R strains, MIC50 values were > 6 µg/mL and > 12 µg/mL for human and animal isolates, respectively. mcr genes were detected in 110 (76.9%) bacterial strains, of which 108 (98.2%) were mcr-1 and 2 (1.8%) were mcr-2.

Conclusions

The detection of a considerable number of mcr-1 and mcr-2 genes in animals is worrisome, as they are now being detected in clinical pathogens. The acquisition of mcr genes by colistin-susceptible bacteria could leave us in a post-antibiotic era.
Vaccines to prevent COVID-19: a protocol for a living systematic review with network meta-analysis including individual patient data (The LIVING VACCINE Project)
Authors: Korang Steven Kwasi , Juul Sophie , Nielsen Emil Eik , Feinberg Joshua , Siddiqui Faiza , Ong Giok , Klingenberg Sarah , Veroniki Areti Angeliki , Bu Fanlong , Thabane Lehana , Thomsen Allan Randrup , Jakobsen Janus C. , Gluud Christian
Published: November 2020

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19) which has rapidly spread worldwide. Several human randomized clinical trials assessing potential vaccines are currently underway. There is an urgent need for a living systematic review that continuously assesses the beneficial and harmful effects of all available vaccines for COVID-19.

Methods/design

We will conduct a living systematic review based on searches of major medical databases (e.g., MEDLINE, EMBASE, CENTRAL) and clinical trial registries from their inception onwards to identify relevant randomized clinical trials. We will update the literature search once a week to continuously assess if new evidence is available. Two review authors will independently extract data and conduct risk of bias assessments. We will include randomized clinical trials comparing any vaccine aiming to prevent COVID-19 (including but not limited to messenger RNA; DNA; non-replicating viral vector; replicating viral vector; inactivated virus; protein subunit; dendritic cell; other vaccines) with any comparator (placebo; “active placebo;” no intervention; standard care; an “active” intervention; another vaccine for COVID-19) for participants in all age groups.

Primary outcomes will be all-cause mortality; a diagnosis of COVID-19; and serious adverse events. Secondary outcomes will be quality of life and non-serious adverse events. The living systematic review will include aggregate data meta-analyses, trial sequential analyses, network meta-analyses, and individual patient data meta-analyses. Within-study bias will be assessed using Cochrane risk of bias tool. The Grading of Recommendations, Assessment, Development and Evaluations (GRADE) and Confidence in Network Meta-Analysis (CINeMA) approaches will be used to assess certainty of evidence. Observational studies describing harms identified during the search for trials will also be included and described and analyzed separately.

Discussion

COVID-19 has become a pandemic with substantial mortality. A living systematic review assessing the beneficial and harmful effects of different vaccines is urgently needed. This living systematic review will regularly inform best practice in vaccine prevention and clinical research of this highly prevalent disease.

Systematic review registration

PROSPERO CRD42020196492
Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility
Authors: Nakamura Masayuki , Aizawa Hachidai , Kawabata Hideo , Sato Atsushi , Watanabe Taisuke , Isobe Kazushige , Kitamura Yutaka , Tanaka Takaaki , Kawase Tomoyuki
Published: November 2020

Background

Platelet-rich plasma (PRP) is often used to improve surface biocompatibility. We previously found that platelets rapidly adhere to plain commercially pure titanium (cp-Ti) plates in the absence, but not in the presence, of plasma proteins. To further expand on these findings, in the present study, we switched titanium plates from a plain surface to a rough surface that is blasted with calcium phosphate (CaP) powder and then examined platelet adhesion and activation.

Methods

Elemental distribution in CaP-blasted cp-Ti plates was analyzed using energy-dispersive X-ray spectroscopy. PRP samples prepared from anticoagulated blood samples of six healthy, non-smoking adult male donors were loaded on CaP-blasted cp-Ti plates for 1 h and fixed for examination of platelet morphology and visualization of PDGF-B and platelet surface markers (CD62P, CD63) using scanning electron microscopy and fluorescence microscopy. Plain SUS316L stainless steel plates used in injection needles were also examined for comparison.

Results

Significant amounts of calcium and phosphate were detected on the CaP-blasted cp-Ti surface. Platelets rapidly adhered to this surface, leading to higher activation. Platelets also adhered to the plain stainless surface; however, the levels of adhesion and activation were much lower than those observed on the CaP-blasted cp-Ti plate.

Conclusions

The CaP-blasted cp-Ti surface efficiently entraps and activates platelets. Biomolecules released from the activated platelets could be retained by the fibrin matrix on the surface to facilitate regeneration of the surrounding tissues. Thus, PRP immersion could not only eliminate surface air bubbles but also improve the biocompatibility of the implant surface.
Unmet need for family planning in Ethiopia and its association with occupational status of women and discussion to her partner: a systematic review and meta-analysis
Authors: Worku Solomon Adanew , Mittiku Yohannes Moges , Wubetu Abate Dargie
Published: November 2020

Background

Unmet need refers to fecund women who either wish to postpone the next birth (spacers) or who wish to stop childbearing (limiters) but are not using a contraceptive method. Many women who are sexually active would prefer to avoid becoming pregnant but are not using any method of contraception. These women are considered to have an unmet need for family planning. Therefore, the objective of this systematic review and meta-analysis is to estimate the pooled prevalence of unmet need for family planning and its association to occupational status of women and discussion to her partner among fecund women in Ethiopia.

Method

A systemic review and meta-analysis was conducted using published and unpublished research on the prevalence of unmet need for family planning and its association to occupational status of women and discussion to her partner among fecund women in Ethiopia. Data extraction was designed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Studies were accessed through electronic web-based search from PubMed, Cochrane Library, Google Scholar, CINAHL, and Embase. All statistical analysis were done using STATA version 14 software using random effects model. The pooled prevalence was presented in forest plots.

Results

A total of 9 studies with 9785 participants were included, and the overall pooled estimated prevalence of unmet need for family planning among fecund women in Ethiopia was 34.90% (95% CI: 24.52, 45.28%). According to subgroup analysis the estimated prevalence of unmet need for family planning in studies conducted in Amhara was 32.98% (95% CI: 21.70, 44.26%), and among married women was 32.84% (95% CI: 16.62, 49.07%). Additionally, housewife women were 1.6 times more likely have unmet need for family planning compared to government employed women (OR: 1.6, 95% CI: 1.29, 1.99). Moreover, women who don’t discuss to partner were 1.87 times more likely to have unmet need for family planning compared to women who had discussion to her partner (OR 1.87; 95% CI: 1.52, 2.31).

Conclusion

The analysis revealed that the overall prevalence of unmet need for family planning among fecund women in Ethiopia was high. Family planning programs should identify strategies to improve communication in family planning among couples and to ensure better cooperation between partners.
Medicine recalls in Saudi Arabia: a retrospective review of drug alerts (January 2010–January 2019)
Authors: AlQuadeib Bushra T. , Alfagih Iman M. , Alnahdi Ameera H. , Alharbi Shadia M. , Al-ahmari Rawan A.
Published: November 2020

Background

A new incidence of a substandard medicinal product is discovered weekly as stated by the World Health Organization (WHO) around the world. After discovering an incidence of a substandard medicinal product, a drug recall announced to remove the affected medicinal product from the market. Drug recalls in SaudiArabia (SA) are made by the Saudi Food and Drug Authority (SFDA) or the distributors of the drug when certain criteria are met. A retrospective study of drug recalls in SA was carried out. Data were collected through a website search of the SFDA for drug recalls since January 2010–January 2019.

Results

SFDA recalled 84 substandard medicinal products which related to 47 alert letters and 52 drugs. The number of drug recall reported by SFDA increased six-folds in 2018 than in 2010. The major frequent therapeutic class of drug recall was the antihypertensive drugs followed by the antibiotic drugs (27.7% and 10.8%, respectively). The majority of the recalls were tablets followed by parenteral dosage forms (58% and 25%, respectively). The major reasons for the drug recalls were due to contamination (32.14%) followed by non-compliance with manufacturer’s specifications (20.48%). Two manufacturers were accounted for 34.52% of all recalled drugs.

Conclusion

Substandard medicinal products are considered as a serious problem in SA. Contamination was the main cause of drug recalls, which requires root investigation of causes and for strict protective tools to be applied by drug manufacturers.
Advancing Science while Training Undergraduates: Recommendations from a Collaborative Biology Research Network
Authors: Jensen-Ryan Danielle , Murren Courtney J. , Rutter Matthew T. , Thompson Jennifer Jo
Published: November 2020

This essay investigates the interplay of scientific and pedagogical objectives within an undergraduate-focused network research project. The multiple priorities faculty face in mentoring emerging scientists while producing high-quality data are discussed and pragmatic recommendations to support effective undergraduate-focused research networks are offered.
Denosumab Safety and Efficacy Among Participants in the FREEDOM Extension Study With Mild to Moderate Chronic Kidney Disease
Authors: Broadwell Aaron , Chines Arkadi , Ebeling Peter R , Franek Edward , Huang Shuang , Smith Shawna , Kendler David , Messina Osvaldo , Miller Paul D
Published: November 2020

Context

The effects of long-term exposure to denosumab in individuals with renal insufficiency are unknown.

Objective

This post hoc analysis evaluates the long-term safety and efficacy of denosumab in individuals with mild-to-moderate chronic kidney disease (CKD) (stages 2 and 3) using data from the pivotal phase 3, double-blind, 3-year FREEDOM (NCT00089791) and open-label, 7-year extension (NCT00523341) studies.

Participants and Methods

Women age 60 to 90 years with a bone mineral density (BMD) T-score of less than –2.5 to greater than –4.0 at the total hip or lumbar spine were randomly assigned 1:1 to receive denosumab 60 mg subcutaneously every 6 months (long-term arm) or placebo (cross-over arm) in FREEDOM; eligible participants could enroll in the extension to receive denosumab 60 mg subcutaneously every 6 months. Change in estimated glomerular filtration rate (eGFR) from study baseline and annualized rates of fracture and adverse events (AEs) were the main outcome measures.

Results

Most participants (1259/1969 [64%] long-term arm; 1173/1781 [66%] crossover arm) with baseline CKD stage 2 or 3 remained within the same CKD subgroup at study completion; less than 3% progressed to CKD stage 4. Participants in all eGFR subgroups showed similar, persistent BMD gains over time and a low incidence of fractures. The percentage of participants reporting serious AEs was similar among renal subgroups (normal, CKD stage 2, CKD stage 3a, CKD stage 3b) both for the long-term (54% vs 52% vs 57% vs 58%) and crossover (43% vs 42% vs 43% vs 68%) arms, except CKD stage 3b subgroup, crossover arm.

Conclusion

The safety and efficacy of denosumab did not differ among participants with mild to moderate CKD.
Prostate-Specific Deletion of Cdh1 Induces Murine Prostatic Inflammation and Bladder Overactivity
Authors: Pascal Laura E , Mizoguchi Shinsuke , Chen Wei , Rigatti Lora H , Igarashi Taro , Dhir Rajiv , Tyagi Pradeep , Wu Zeyu , Yang Zhenyu , de Groat William C , DeFranco Donald B , Yoshimura Naoki , Wang Zhou
Published: November 2020

Benign prostatic hyperplasia (BPH) is an age-related debilitating prostatic disease that is frequently associated with prostatic inflammation and bothersome lower urinary tract symptoms (LUTS). Animal models have shown that formalin- and bacterial-induced prostatic inflammation can induce bladder dysfunction; however, the underlying mechanisms contributing to prostatic inflammation in BPH and bladder dysfunction are not clear. We previously reported that E-cadherin expression in BPH is downregulated in hyperplastic nodules compared with expression in adjacent normal tissues. Here, we explored the potential consequences of prostatic E-cadherin downregulation on the prostate and bladder in vivo using an inducible murine model of prostate luminal epithelial-specific deletion of Cdh1. The prostate-specific antigen (PSA)-CreERT2 transgenic mouse strain expressing tamoxifen-inducible CreERT2 recombinase driven by a 6-kb human PSA promoter/enhancer was crossed with the B6.129-Cdh1tm2Kem/J mouse to generate bigenic PSA-CreERT2/Cdh1-/- mice. Deletion of E-cadherin was induced by transient administration of tamoxifen when mice reached sexual maturity (7 weeks of age). At 21 to 23 weeks of age, the prostate, bladder, and prostatic urethra were examined histologically, and bladder function was assessed using void spot assays and cystometry. Mice with Cdh1 deletion had increased prostatic inflammation, prostatic epithelial hyperplasia, and stromal changes at 21 to 23 weeks of age, as well as changes in bladder voiding function compared with age-matched controls. Thus, loss of E-cadherin in the murine prostate could result in prostatic defects that are characteristic of BPH and LUTS, suggesting that E-cadherin downregulation could be a driving force in human BPH development and progression.
Posttranscriptional modifications at the 37th position in the anticodon stem–loop of tRNA: structural insights from MD simulations
Authors: Seelam Prabhakar Preethi , Takyi Nathania A. , Wetmore Stacey D.
Published: November 2020

Transfer RNA (tRNA) is the most diversely modified RNA. Although the strictly conserved purine position 37 in the anticodon stem–loop undergoes modifications that are phylogenetically distributed, we do not yet fully understand the roles of these modifications. Therefore, molecular dynamics simulations are used to provide molecular-level details for how such modifications impact the structure and function of tRNA. A focus is placed on three hypermodified base families that include the parent i6A, t6A, and yW modifications, as well as derivatives. Our data reveal that the hypermodifications exhibit significant conformational flexibility in tRNA, which can be modulated by additional chemical functionalization. Although the overall structure of the tRNA anticodon stem remains intact regardless of the modification considered, the anticodon loop must rearrange to accommodate the bulky, dynamic hypermodifications, which includes changes in the nucleotide glycosidic and backbone conformations, and enhanced or completely new nucleobase–nucleobase interactions compared to unmodified tRNA or tRNA containing smaller (m1G) modifications at the 37th position. Importantly, the extent of the changes in the anticodon loop is influenced by the addition of small functional groups to parent modifications, implying each substituent can further fine-tune tRNA structure. Although the dominant conformation of the ASL is achieved in different ways for each modification, the molecular features of all modified tRNA drive the ASL domain to adopt the functional open-loop conformation. Importantly, the impact of the hypermodifications is preserved in different sequence contexts. These findings highlight the likely role of regulating mRNA structure and translation.
Completeness of reporting acupuncture interventions for chronic obstructive pulmonary disease: Review of adherence to the STRICTA statement
Authors: Fernández-Jané Carles , Solà-Madurell Mireia , Yu Mingkun , Liang Changhao , Fei Yutong , Sitjà-Rabert Mercè , Úrrutia Gerard
Published: November 2020

Background: The completeness of reporting  of acupuncture interventions is critical to ensure the applicability and reproducibility of acupuncture clinical trials. In the past, different publications have evaluated the completeness of reporting of acupuncture interventions for different clinical situations, such as knee osteoarthritis, neurological diseases or cancer. However, this has not been done for acupuncture trials for chronic obstructive pulmonary disease (COPD).

Objective: To assess the completeness of reporting of acupuncture interventions in trials for COPD.

Methods: A total of 11 English and Chinese databases were screened up until May 2019 for randomised or quasi-randomised control trials of acupuncture for COPD. The STRICTA checklist was used to determine the quality of the reporting of acupuncture interventions.

Results: A total of 28 trials were included in our review. Out of the 16 STRICTA checklist subitems analysed, only 4 were considered appropriately reported in more than 70% of the trials, while 7 were correctly reported in less than 30%.

Conclusion: The adherence to STRICTA guidelines of acupuncture trials for COPD is suboptimal, and future efforts need to be addressed to improve the completeness of reporting.
Case Report: Unusual presentation of concomitant gastric outlet obstruction and jaundice complicating giant hepatic angioma
Authors: Elleuch Nour , Hammami Aya , Hassine Amira , Ben Ameur Wafa , Dahmani Wafa , Aida Ben Slama , Mehdi Ksiaa , Ahlem Braham , Salem Ajmi , Jaziri Hanen , Ali Jmaa
Published: November 2020

Hemangioma is the most common benign tumor affecting the liver.  The vast majority of liver hemangiomas (LH) are less than 30 mm in diameter, asymptomatic, and are most often identified incidentally during radiological investigations for other reasons. Giant LH greater than 50 mm can lead to the development of symptoms and complications that require prompt surgical intervention. Herein, we report the case of a 58-year-old man who presented with gastric outlet obstruction and obstructive jaundice as a result of a giant hepatic hemangioma that was complicated with fatal spontaneous rupture.
Comparison of two lipid emulsions on interleukin-1β, interleukin-8 and fatty acid composition in infants post gastrointestinal surgery: a randomized trial
Authors: Hanindita Meta Herdiana , Irawan Roedi , Ugrasena I Dewa Gede , Hariastawa I. G. B. Adria
Published: November 2020

Background: Nutritional support plays an essential role for recovery in infants who undergo gastrointestinal surgery. The current standard type of intravenous lipid emulsion (IVLE) used as parenteral nutrition is the mixture of medium-chain triglyceride (MCT) and long chain triglyceride (LCT) rich in ω-6. Studies showed that ω-6 is associated with higher level of proinflammatory cytokines, leading to increased mortality rate, morbidity rate, and postoperative recovery time. The latest generation of emulsion is a mixture of MCT, LCT, olive oil (OO), and fish oil (FO) which may optimize the ω6/ω3 ratio. This study aimed to compare the effect of MCT/LCT/OO/FO IVLE to standard IVLE on IL-1β, IL-8 and plasma fatty acids in infants who had undergone gastrointestinal surgery.

Methods: A single-blind, randomised controlled, pretest-posttest design study was done in twelve subjects that were classified into two groups. Group 1 received standard IVLE, group 2 received MCT/LCT/OO/FO IVLE. The type of standard and MCT/LCT/OO/FO IVLE used in this study were Lipofundin 20% and SMOFlipid 20%, respectively, both administered for three consecutive days in 1-4 gram/kilogram/day. IL-1β and IL-8 were examined using ELISA while fatty acids was analyzed using gas chromatography tandem mass spectrometry (GC-MS).  Statistical analyses were performed using SPSS for Mac 23.

Results: No statistical difference was found in age, gender, birth weight and diagnosis between both groups. Leukocyte was significantly lower in MCT/LCT/OO/FO group 3 days after surgery (p=0.025). CRP was lower in MCT/LCT/OO/FO group 3 days after surgery (p=0.01) and in changes within 3 days (p=0.016). There were no differences in IL-1β, IL-8 and ω-3 but ω-6 was higher in standard IVFE group on third day after surgery (p=0,048)

Conclusion: MCT/LCT/OO/FO IVLE can significantly lower leukocyte, CRP and ω-6 levels and is comparable with standard IVLE on IL-1β, IL-8 and ω-3 levels in infants underwent gastrointestinal surgery.
Studying the prominence effect amid the COVID-19 crisis: implications for public health policy decision-making.
Authors: Maaravi Yossi , Heller Ben
Published: November 2020

The novel coronavirus disease 2019 (COVID-19) has brought with it crucial policy- and decision-making situations, especially when making judgments between economic and health concerns. One particularly relevant decision-making phenomenon is the prominence effect, where decision-makers base their decisions on the most prominent attribute of the object at hand (e.g., health concerns) rather than weigh all the attributes together. This bias diminishes when the decision-making mode inhibits heuristic processes. In this study, we tested the prominence of health vs. economic concerns across two decision-making modes - choice (prone to heuristics) and matching (mitigates heuristics) - during the peak of the COVID-19 in the UK using Tversky et al.’s classic experimental paradigm. We added to the classic experimental design a priming condition. Participants were presented with two casualty-minimization programs, differing in lives saved and costs: program X would save 100 lives at the cost of 55-million-pound sterling, whereas program Y would save 30 lives at the cost of 12-million-pound sterling. Half of the participants were required to choose between the programs (choice condition). The other half were not given the cost of program X and were asked to determine what the cost should be to make it as equally attractive as the program Y. Participants in both groups were primed for either: a) economic concerns; b) health concerns; or c) control (no priming). Results showed that in the choice condition, unless primed for economic concerns, health concerns are more prominent. In the matching condition, on the other hand, the prominence of health concerns did not affect decision-makers, as they all “preferred” the cheaper option. These results add further support to the practical relevance of using the proper decision-making modes in times of consequential crises where multiple concerns, interests, and parties are involved.
The Avon Longitudinal Study of Parents and Children - A resource for COVID-19 research: Questionnaire data capture April-May 2020
Authors: Northstone Kate , Howarth Simown , Smith Daniel , Bowring Claire , Wells Nicholas , Timpson Nicholas John
Published: November 2020

The Avon Longitudinal Study of Parents and Children (ALSPAC) is a prospective population-based cohort study which recruited pregnant women in 1990-1992. The resource provides an informative and efficient setting for collecting data on the current coronavirus 2019 (COVID-19) pandemic. In early March 2020, a questionnaire was developed in collaboration with other longitudinal population studies to ensure cross-cohort comparability. It targeted retrospective and current COVID-19 infection information (exposure assessment, symptom tracking and reported clinical outcomes) and the impact of both disease and mitigating measures implemented to manage the COVID-19 crisis more broadly. Data were collected on symptoms of COVID-19 and seasonal flu, travel prior to the pandemic, mental health and social, behavioural and lifestyle factors.

The online questionnaire was deployed across parent (G0) and offspring (G1) generations between 9th April and 15th May 2020. 6807 participants completed the questionnaire (2706 original mothers, 1014 original fathers/partners, 2973 offspring (mean age ~28 years) and 114 offspring partners). Eight (0.01%) participants (4 G0 and 4 G1) reported a positive test for COVID-19, 77 (1.13%; 28 G0 and 49 G1) reported that they had been told by a doctor they likely had COVID-19 and 865 (12.7%; 426 G0 and 439 G1) suspected that they have had COVID-19.  Using algorithmically defined cases, we estimate that the predicted proportion of COVID-19 cases ranged from 1.03% - 4.19% depending on timing during the period of reporting (October 2019-March 2020).

Data from this first questionnaire will be complemented with at least two more follow-up questionnaires, linkage to health records and results of biological testing as they become available. Data has been released as: 1) a standard dataset containing all participant responses with key sociodemographic factors and 2) as a composite release coordinating data from the existing resource, thus enabling bespoke research across all areas supported by the study.
The Diagnostic Value of the Ulnocarpal Stress Test for Differentiating the Symptomatic Triangular Fibrocartilage Complex Tear from the Incidental Tear
Authors: Kim Cheol-U , Kim Dong Hyun , Kwon Bong Cheol
Published: November 2020

Purpose: The purpose of this study was to determine the diagnostic value of the ulnocarpal stress test (UCST) for differentiating the symptomatic triangular fibrocartilage complex (TFCC) tear from the incidental tear.Methods: From January 2013 to May 2020, a study was performed on 109 patients who underwent wrist arthroscopy and showed TFCC tear. Patients who had ulnar-sided wrist pain along with TFCC tear, and showed postoperative symptom improvement, and those with other lesions excluded were defined as symptomatic tear (experimental group) and the rest were defined as asymptomatic tear (control group). Diagnostic values of the UCST for differentiating the symptomatic TFCC tear from the incidental tear were calculated.Results: The UCST showed a sensitivity of 54%, a specificity of 95%, a positive predictive value of 98%, a negative predictive value of 31%, a positive likelihood ratio of 10.34, a negative likelihood ratio of 0.48, and an overall diagnostic accuracy of 61%. Conclusion: The UCST showed limited diagnostic value for symptomatic TFCC tear. Therefore, this test needs to be regarded as a part of diagnostic process and their results should be interpreted in the context of other relevant clinical information before reaching the diagnosis of symptomatic TFCC tear.
Frontiers in Service Science: The Management of Data Analytics Services: New Challenges and Future Directions
Authors: de Véricourt Francis , Perakis Georgia
Published: November 2020

In this short paper, we discuss the impact of data analytics in services and delineate future research directions for the field. After illustrating how data analytics are transforming different service sectors, we consider the provision of data analysis as a service in its own right. We discuss how the very nature of data and certain features of the machine learning method give rise to new issues and pitfalls for the management of these services, which delineates as many future research directions. We also discuss the coproduction of services by humans and machines and call for more research on responsible data analytics services to tackle some of the most pressing ethical issues in our societies.
Coming of age? On the professionalisation of social work in Israeli-Arab society in the new millennium
Authors: Mahajne Ibrahim , Bar-On Arnon Alexander
Published: November 2020

The article traces social work’s development in Israel’s Palestinian society from 2007 until a reform of the welfare bureaus in 2018, based on primary and secondary written sources, interviews with Palestinian social workers employed at the time, and a survey of social workers throughout the country’s Palestinian local authorities. Despite gains, social work in this society continued to face historical government-based obstacles to its professionalisation, namely, significantly reduced resources compared to its Jewish counterpart, absence of the Palestinian narrative in service provision and lack of Palestinian representation in policy formulation. The result was a continuing dual welfare system: one for the country’s Jewish citizens; and a significantly more restricted one for their Palestinian compatriots.
Broadband microwave photonic frequency translator with a wide frequency shifting range
Authors: Wang Yuzhi , Chen Hao , Chan Erwin H. W.
Published: November 2020

A microwave photonic topology for shifting the frequency of an input microwave signal is presented. It operates based on a single sideband frequency mixing approach. The amount of microwave signal frequency shift is determined by a local oscillator frequency. The proposed frequency translator (FT) has a large bandwidth and a wide frequency shifting range. It can be designed to obtain a large spurious signal suppression ratio and a low frequency translation loss. Results are presented for the novel structure, which demonstrates the realization of a 2–18 GHz FT with a 10 kHz to 100 MHz frequency shifting range. The results also show the spurious signals are more than 31 dB below the frequencyshifted signal, and a low loss of only around 4 dB for frequency shifting a 10 GHz microwave signal.
Spatial axial shearing common-path interferometer for natural light
Authors: Imbe Masatoshi
Published: November 2020

A spatial axial shearing interferometer is proposed to obtain a mutual coherence function representing longitudinal spatial coherence of natural light. The modulation of the quadratic phase distribution displayed on a spatial light modulator generates a spatial axial shear without a radial one. Because the optical path lengths along the optical axis on the two paths are identical, the spatial axial shear can be greater than the coherence length derived by temporal coherence. Experimental results are given to confirm that the mutual coherence function obtained by the proposed interferometer has spatial distribution expected by the relation between coherence and diffraction formula.
Ultracompact silicon polarization splitter-rotator using dual-etched and tapered coupler: publisher’s note
Authors: Xie Changjian , Zou Xihua , Li Peixuan , Yan Lianshan , Pan Wei
Published: November 2020

This publisher’s note corrects the Funding section in Appl. Opt.
59, 9540 (2020)APOPAI0003-693510.1364/AO.404741.
Nearly perfect transmission of unpolarized infrared radiation through a one-dimensional metal grating embedded in a monolithic high-contrast grating
Authors: Sokół Adam K. , Czyszanowski Tomasz
Published: November 2020

We demonstrate a conceptually simple polarization-independent mechanism for nearly perfect infrared light transmission through a subwavelength one-dimensional metal grating implemented in the grooves of a deep-subwavelength monolithic high-contrast grating (metalMHCG). We provide theoretical background explaining the transmission mechanism, which eliminates Fresnel reflection as well as significantly reduces metal absorption and the reflection of transverse electric and transverse magnetic light polarizations. Careful design of a metalMHCG implemented at the interface between the regions of high refractive index contrast enables the coincidence of high transmission conditions for both light polarizations, enabling up to 97% transmission of polarization-independent infrared radiation. Our analysis shows excellent electrical properties of the metalMHCG as evidenced by sheet resistance of 2 ΩSq−1 facilitating straightforward horizontal electron transport and vertical injection of the current into the semiconductor substrate on which the electrode is implemented.
Refractive index sensing with optical bound states in the continuum
Authors: Maksimov Dmitrii N. , Gerasimov Valeriy S. , Romano Silvia , Polyutov Sergey P.
Published: November 2020

We consider refractive index sensing with optical bounds states in the continuum (BICs) in dielectric gratings. Applying a perturbative approach we derived the differential sensitivity and the figure of merit of a sensor operating in the spectral vicinity of a BIC. Optimisation design approach for engineering an effective sensor is proposed. An analytic formula for the maximal sensitivity with an optical BIC is derived. The results are supplied with straightforward numerical simulations.
Watt-level visible laser in double-clad Pr3+-doped fluoride fiber pumped by a GaN diode
Authors: Kifle Esrom , Starecki Florent , Loiko Pavel , Cozic Solenn , Joulain Franck , Berthelot Thibaud , Georges Thierry , Stojcevski Dragan , Deubel Damien , Camy Patrice
Published: November 2020

We report on a red praseodymium fiber laser delivering 1.07 W at 634.5 nm with a slope efficiency of 20.7% (versus the incident pump), a laser threshold of 0.55 W, and a single-mode output (

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in the quasi-continuous-wave regime. It is based on a 0.6 mol.%

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-doped ZBLAN double-clad fiber with a 5.5 µm core, a double D-shaped (diameters, 115/125 µm) inner cladding, and a length of 5.0 m. The fiber is pumped by a multimode 443 nm GaN diode. The laser design is optimized using a numerical model. The proposed concept is suitable for the development of diode-pumped high brightness watt-level visible praseodymium fiber lasers.
Smart ring resonator–based sensor for multicomponent chemical analysis via machine learning
Authors: Li Zhenyu , Zhang Hui , Nguyen Binh Thi Thanh , Luo Shaobo , Liu Patricia Yang , Zou Jun , Shi Yuzhi , Cai Hong , Yang Zhenchuan , Jin Yufeng , Hao Yilong , Zhang Yi , Liu Ai-Qun
Published: November 2020

We demonstrate a smart sensor for label-free multicomponent chemical analysis using a single label-free ring resonator to acquire the entire resonant spectrum of the mixture and a neural network model to predict the composition for multicomponent analysis. The smart sensor shows a high prediction accuracy with a low root-mean-squared error ranging only from 0.13 to 2.28 mg/mL. The predicted concentrations of each component in the testing dataset almost all fall within the 95% prediction bands. With its simple label-free detection strategy and high accuracy, the smart sensor promises great potential for multicomponent analysis applications in many fields.
Heparan Sulfate Biosynthesis in Zebrafish
Authors: Filipek-Górniok Beata , Habicher Judith , Ledin Johan , Kjellén Lena
Published: November 2020

The biosynthesis of heparan sulfate (HS) proteoglycans occurs in the Golgi compartment of cells and will determine the sulfation pattern of HS chains, which in turn will have a large impact on the biological activity of the proteoglycans. Earlier studies in mice have demonstrated the importance of HS for embryonic development. In this review, the enzymes participating in zebrafish HS biosynthesis, along with a description of enzyme mutants available for functional studies, are presented. The consequences of the zebrafish genome duplication and maternal transcript contribution are briefly discussed as are the possibilities of CRISPR/Cas9 methodologies to use the zebrafish model system for studies of biosynthesis as well as proteoglycan biology.
Novel small RNAs expressed by Bartonella bacilliformis under multiple conditions reveal potential mechanisms for persistence in the sand fly vector and human host
Authors: Wachter Shaun , Hicks Linda D. , Raghavan Rahul , Minnick Michael F.
Published: November 2020

Bartonella bacilliformis, the etiological agent of Carrión’s disease, is a Gram-negative, facultative intracellular alphaproteobacterium. Carrión’s disease is an emerging but neglected tropical illness endemic to Peru, Colombia, and Ecuador.B.bacilliformisis spread between humans through the bite of female phlebotomine sand flies. As a result, the pathogen encounters significant and repeated environmental shifts during its life cycle, including changes in pH and temperature. In most bacteria, small non-coding RNAs (sRNAs) serve as effectors that may post-transcriptionally regulate the stress response to such changes. However, sRNAs have not been characterized inB.bacilliformis, to date. We therefore performed total RNA-sequencing analyses onB.bacilliformisgrownin vitrothen shifted to one of ten distinct conditions that simulate various environments encountered by the pathogen during its life cycle. From this, we identified 160 sRNAs significantly expressed under at least one of the conditions tested. sRNAs included the highly-conserved tmRNA, 6S RNA, RNase P RNA component, SRP RNA component,ffHleader RNA, and the alphaproteobacterial sRNAs αr45 andspeFleader RNA. In addition, 153 other potential sRNAs of unknown function were discovered. Northern blot analysis was used to confirm the expression of eight novel sRNAs. We also characterized aBartonellabacilliformisgroup Iintron (BbgpI) that disrupts an un-annotated tRNACCUArggene and determined that the intron splicesin vivoand self-splicesin vitro. Furthermore, we demonstrated the molecular targeting ofBartonellabacilliformissmallRNA9(BbsR9) to transcripts of theftsH,nuoF, andgcvTgenes,in vitro.
Proteomics of extracellular vesicles produced by Granulicatella adiacens, which causes infective endocarditis
Authors: Alkandari Sarah A. , Bhardwaj Radhika G. , Ellepola Arjuna , Karched Maribasappa
Published: November 2020

When oral bacteria accidentally enter the bloodstream due to transient tissue damage during dental procedures, they have the potential to attach to the endocardium or an equivalent surface of an indwelling prosthesis and cause infection. Many bacterial species produce extracellular vesicles (EVs) as part of normal physiology, but also use it as a virulence strategy. In this study, it was hypothesized that Granulicatella adiacens produce EVs that possibly help it in virulence. Therefore, the objectives were to isolate and characterize EVs produced by G. adiacens and to investigate its immune-stimulatory effects. The reference strain G. adiacens CCUG 27809 was cultured on chocolate blood agar for 2 days. From subsequent broth culture, the EVs were isolated using differential centrifugation and filtration protocol and then observed using scanning electron microscopy. Proteins in the vesicle preparation were identified by nano LC-ESI-MS/MS. The EVs proteome was analyzed and characterized using different bioinformatics tools. The immune-stimulatory effect of the EVs was studied via ELISA quantification of IL-8, IL-1β and CCL5, major proinflammatory cytokines, produced from stimulated human PBMCs. It was revealed that G. adiacens produced EVs, ranging in diameter from 30 to 250 nm. Overall, G. adiacens EVs contained 112 proteins. The proteome consists of several ribosomal proteins, DNA associated proteins, binding proteins, and metabolic enzymes. It was also shown that these EVs carry putative virulence factors including moonlighting proteins. These EVs were able to induce the production of IL-8, IL-1β and CCL5 from human PBMCs. Further functional characterization of the G. adiacens EVs may provide new insights into virulence mechanisms of this important but less studied oral bacterial species.
Optimal vehicle size and driving condition for extended-range electric vehicles in China: A life cycle perspective
Authors: Liu Yongtao , Qiao Jie , Xu Haibo , Liu Jiahui , Chen Yisong
Published: November 2020

Many researchers use life cycle assessment methodology to investigate the energy and environmental impacts of energy-saving and new energy vehicles. However, in the context of China, the life cycle energy-saving and emission-reduction effects of extended-range electric vehicles (EREVs), and the optimal applicable vehicle size and driving conditions for EREVs have been rarely studied. In this study, based on the life cycle assessment theory, the resource consumption, energy exhaustion, and environmental impact of EREVs were comprehensively analyzed. In addition, a differential evaluation model of ecological benefits was established for comparing EREVs with other vehicles with different power sources. Finally, scenario analysis was performed in terms of different vehicle sizes and driving conditions. The results have shown that EREV has great advantages in reducing mineral resource consumption and fossil energy consumption. The consumption of mineral resources of EREV is 14.68% lower than that of HEV, and the consumption of fossil energy is 34.72% lower than that of ICEV. In terms of environmental impact, EREV lies in the middle position. The scenario analysis has revealed that, for EREV in China, the optimal vehicle size is the passenger car and the optimal driving condition is the suburban condition. This work helps to understand the environmental performance of EREVs in China and may provide a decision-making reference for the government.