bluuebunny/crossref_metadata_2025 huggingface dataset:
Uterine Nodal expression supports maternal immunotolerance and establishment of the FOXP3+ regulatory T cell population during the preimplantation period
Authors: Yull Sarah , Shafiei Shiva , Park Craig B. , Kazemi Parinaz , Tiemann Emily B. , Pagé Marie-Hélène Godin , Dufort Daniel
Published: October 2023

Pregnancy success is dependent on the establishment of maternal tolerance during the preimplantation period. The immunosuppressive function of regulatory T cells is critical to limit inflammation arising from implantation of the semi-allogeneic blastocyst. Insufficient maternal immune adaptations to pregnancy have been frequently associated with cases of female infertility and recurrent implantation failure. The role of Nodal, a secreted morphogen of the TGFβ superfamily, was recently implicated during murine pregnancy as its conditional deletion (NodalΔ/Δ) in the female reproductive tract resulted in severe subfertility. Here, it was determined that despite normal preimplantation processes and healthy, viable embryos, NodalΔ/Δ females had a 50% implantation failure rate compared to NodalloxP/loxP controls. Prior to implantation, the expression of inflammatory cytokines MCP-1, G-CSF, IFN-γ and IL-10 was dysregulated in the NodalΔ/Δ uterus. Further analysis of the preimplantation leukocyte populations in NodalΔ/Δ uteri showed an overabundance of infiltrating, pro-inflammatory CD11bhigh Ly6C+ macrophages coupled with the absence of CD4+ FOXP3+ regulatory T cells. Therefore, it is proposed that uterine Nodal expression during the preimplantation period has a novel role in the establishment of maternal immunotolerance, and its dysregulation should be considered as a potential contributor to cases of female infertility and recurrent implantation failure.
Structural informatics approach for designing an epitope-based vaccine against the brain-eating Naegleria fowleri
Authors: Sarfraz Asifa , Wara Tehreem Ul , Sheheryar  , Chen Ke , Ansari Shahid Habib , Zaman Aqal , Nishan Umar , Iqbal Anwar , Ullah Riaz , Ali Essam A. , Shah Mohibullah , Ojha Suvash Chandra
Published: October 2023

Primary Amoebic Meningoencephalitis (PAM), a severe lethal brain disease, is caused by a parasite, Naegleria fowleri, also known as the “brain-eating amoeba”. The chances of a patient’s recovery after being affected by this parasite are very low. Only 5% of people are known to survive this life-threatening infection. Despite the fact that N. fowleri causes a severe, fatal infection, there is no proper treatment available to prevent or cure it. In this context, it is necessary to formulate a potential vaccine that could be able to combat N. fowleri infection. The current study aimed at developing a multi-epitope subunit vaccine against N. fowleri by utilizing immunoinformatics techniques and reverse vaccinology approaches. The T- and B-cell epitopes were predicted by various tools. In order to choose epitopes with the ability to trigger both T- and B-cell-mediated immune responses, the epitopes were put through a screening pipeline including toxicity, antigenicity, cytokine-inductivity, and allergenicity analysis. Three vaccine constructs were designed from the generated epitopes linked with linkers and adjuvants. The modeled vaccines were docked with the immune receptors, where vaccine-1 showed the highest binding affinity. Binding affinity and stability of the docked complex were confirmed through normal mode analysis and molecular dynamic simulations. Immune simulations developed the immune profile, and in silico cloning affirmed the expression probability of the vaccine construct in Escherichia coli (E. coli) strain K12. This study demonstrates an innovative preventative strategy for the brain-eating amoeba by developing a potential vaccine through immunoinformatics and reverse vaccinology approaches. This study has great preventive potential for Primary Amoebic Meningoencephalitis, and further research is required to assess the efficacy of the designed vaccine.
Real-world evidence for regulatory decision-making: updated guidance from around the world
Authors: Burns Leah , Le Roux Nadege , Kalesnik-Orszulak Robert , Christian Jennifer , Dudinak Jennifer , Rockhold Frank , Khozin Sean , O’Donnell John
Published: October 2023

Leveraging the value of real-world evidence (RWE) to make informed regulatory decisions in the field of health care continues to gain momentum. Improving clinical evidence generation by evaluating the outcomes and patient experiences at the point-of-care would help achieve the ultimate aim of ensuring that effective and safe treatments are rapidly approved for patient use. In our previous publication, we assessed the global regulatory landscape with respect to RWE and provided a review of the regional availability of frameworks and guidance through May 2021 on the basis of 3 key regulatory elements: regulatory RWE frameworks, data quality guidance, and study methods guidance. In the current review, we have updated and elaborated upon recent developments in the regulatory RWE environment from a regional perspective under the same 3 regulatory elements stated above. In addition, we have also included a new category on procedural guidance. The review also discusses the perceived gaps and potential opportunities for future development and harmonization in this field to support framework establishment in regions without pre-existing RWE policies. Additionally, the article reviews current developments of health technology assessment (HTA) bodies pertaining to RWE and discusses the status of evidentiary alignment among regulators and HTA agencies.
Transition from stromatolite to thrombolite fabric: potential role for reticulopodial protists in lake microbialites of a Proterozoic ecosystem analog
Authors: Bernhard Joan M. , Fisher Luke A. , Murphy Quinne , Sen Leena , Yeh Heidi D. , Louyakis Artemis , Gomaa Fatma , Reilly Megan , Batta-Lona Paola G. , Bucklin Ann , Le Roux Veronique , Visscher Pieter T.
Published: October 2023

Prior observations suggest that foraminiferan protists use their reticulopodia (anastomosing pseudopodia) to alter sediment fabric by disrupting laminations of subtidal marine stromatolites, erasing the layered structures in an experimental setting. Because microbialites and foraminifera are found in non-marine settings, we hypothesized that foraminifera living in lakes could also disrupt layered microbialite fabric. With this aim and using a variety of multidisciplinary approaches, we conducted field surveys and an experiment on microbialites from Green Lake (GL; Fayetteville, New York State, United States), which has been studied as a Proterozoic ecosystem analog. The lake is meromictic and alkaline, receiving calcium sulfate-rich water in the monimolimnion; it supports a well-developed carbonate platform that provides access to living and relict microbialites. The living microbialites grow from early spring to autumn, forming a laminated mat at their surface (top ~5 mm), but a clotted or massive structure exists at depth (> ~ 1 cm). We observed a morphotype of “naked” foraminiferan-like protist in samples from GL microbialites and sediments; thus, considered the possibility of freshwater foraminiferan impact on microbialite fabric. Results of an experiment that seeded the cultured freshwater foraminifer Haplomyxa saranae onto the GL microbialite surface indicates via micro-CT scanning and anisotropy analysis that the introduced foraminifer impacted uppermost microbialite layering (n = 3 cores); those cores with an added inhibitor lacked changes in anisotropy for two of those three cores. Thus, it remains plausible that the much smaller, relatively common, native free-form reticulate protist, which we identified as Chlamydomyxa labyrinthuloides, can disrupt microbialite fabrics on sub-millimeter scales. Our observations do not exclude contributions of other possible causal factors.
New insight into the metabolic mechanism of a novel lipid-utilizing and denitrifying bacterium capable of simultaneous removal of nitrogen and grease through transcriptome analysis
Authors: Tong Yaobin , Li Yiyi , Qin Wenpan , Wu Shengchun , Xu Weiping , Jin Peng , Zheng Zhanwang
Published: October 2023

Introduction

Issues related to fat, oil, and grease from kitchen waste (KFOG) in lipid-containing wastewater are intensifying globally. We reported a novel denitrifying bacterium Pseudomonas CYCN-C with lipid-utilizing activity and high nitrogen-removal efficiency. The aim of the present study was aim to explore the metabolic mechanism of the simultaneous lipid-utilizing and denitrifying bacterium CYCN-C at transcriptome level.

Methods

We comparatively investigated the cell-growth and nitrogen-removal performances of newly reported Pseudomonas glycinae CYCN-C under defined cultivation conditions. Transcriptome analysis was further used to investigate all pathway genes involved in nitrogen metabolism, lipid degradation and utilization, and cell growth at mRNA levels.

Results

CYCN-C could directly use fat, oil, and grease from kitchen waste (KFOG) as carbon source with TN removal efficiency of 73.5%, significantly higher than that (60.9%) with sodium acetate. The change levels of genes under defined KFOG and sodium acetate were analyzed by transcriptome sequencing. Results showed that genes cyo, CsrA, PHAs, and FumC involved in carbon metabolism under KFOG were significantly upregulated by 6.9, 0.7, 26.0, and 19.0-folds, respectively. The genes lipA, lipB, glpD, and glpK of lipid metabolic pathway were upregulated by 0.6, 0.4, 21.5, and 1.3-folds, respectively. KFOG also improved the denitrification efficiency by inducing the expression of the genes nar, nirB, nirD, and norR of denitrification pathways.

Conclusion

In summary, this work firstly provides valuable insights into the genes expression of lipid-utilizing and denitrifying bacterium, and provides a new approach for sewage treatment with reuse of KFOG wastes.
The preservation of right cingulum fibers in subjective cognitive decline of preclinical phase of Alzheimer’s disease
Authors: Sun Yu , Qiao Yanan , Guo Jing , Hou Wenjie , Chen Yaojing , Peng Dantao
Published: October 2023

Introduction

Subjective cognitive decline (SCD) with a positive amyloid burden has been recognized as the earliest clinical symptom of the preclinical phase of Alzheimers disease (AD), providing invaluable opportunities to improve our understanding of the natural history of AD and determine strategies for early therapeutic interventions.

Methods

The microstructure of white matter in patients showing SCD in the preclinical phase of AD (SCD of pre-AD) was evaluated using diffusion images, and voxel-wise fractional anisotropy (FA), mean diffusivity (MD), and axial and radial diffusivities were assessed and compared among participant groups. Significant clusters in the tracts were extracted to determine their associations with alterations in the cognitive domains.

Results

We found that individuals with SCD of pre-AD may have subclinical episodic memory impairment associated with the global amyloid burden. Meanwhile, we found significantly reduced FA and λ1 in the right cingulum (cingulate and hippocampus) in AD dementia, while significantly increased FA and decreased MD as well as λ23 in the SCD of pre-AD group in comparison with the HC group.

Discussion

In conclusion, increased white matter microstructural integrity in the right cingulum (cingulate and hippocampus) may indicate compensation for short-term episodic memory in individuals with SCD of pre-AD in comparison with individuals with AD and healthy elderly individuals.
Electroconvulsive seizures regulate various stages of hippocampal cell genesis and mBDNF at different times after treatment in adolescent and adult rats of both sexes
Authors: Ledesma-Corvi Sandra , García-Fuster M. Julia
Published: October 2023

Electroconvulsive therapy, a fast-acting option for treatment-resistant depression, is modeled at the preclinical level through the induction of electroconvulsive seizures (ECS) in rodents. Recent studies from our group proved sex- and age-differences in the antidepressant-like response elicited by ECS in rats; while an antidepressant-like response was observed in male adolescent and adult rats (although with greater efficacy in adulthood), the same parameters rendered inefficacious in females of any age. To better understand the potential sex differences taking place at the molecular level that might be mediating these behavioral disparities, we evaluated the impact of a repeated treatment with ECS (95 mA for 0.6 s, 100 Hz, 0.6 ms) in adolescent and adult rats of both sexes. Several hippocampal markers of neuroplasticity, commonly regulated by most antidepressants, such as those of neurogenesis (cell proliferation, neurogenic differentiation, long-term cell survival) or mBDNF and associated signaling (e.g., mTOR and ERK1/2) were evaluated at different time-points after treatment (1-, 8-, 15- and up to 30-days post-treatment). The main results demonstrated that ECS improved the survival rate of new cells born in the dentate gryus before treatment. Moreover, ECS increased cell proliferation and neurogenic differentiation at different times post-treatment, paired with persistent increases in mBDNF, observed long after treatment. In general, effects were different for each sex and varied with the age of the animal (adolescent vs. adulthood). The present study is the first-one to demonstrate that such persistent molecular changes induced by ECS in hippocampus, some of them observed up to 30-days post-treatment, also occurred in female rats and adolescence. Although these molecular changes could not justify the lack of ECS efficacy described by these same parameters of ECS in female rats (vs. male rats), they proposed certain beneficial effects common to both sexes, and age periods studied, opening the avenue for further studies. Based on these neurochemical effects, ECS should have displayed similar efficacies for both biological sexes. Therefore, the reason behind these disparities should be further explored to better translate efficacious treatments specific and/or personalized for each sex to the clinic.
The protective associations of breastfeeding with infant overweight and asthma are not dependent on maternal FUT2 secretor status
Authors: Manus Melissa B. , Goguen Stephanie K. , Azad Meghan B.
Published: October 2023

Breastfeeding supplies infant gut bacteria with human milk oligosaccharides (HMOs) as a nutrient source. HMO profiles are influenced by the FUT2 gene, which encodes an enzyme affecting the fucosylation of milk sugars. 20 to 40% of individuals have a “non-secretor” polymorphism that inactivates the FUT2 gene, resulting in variable HMO proportions in milk. This has engendered a concerning, yet unfounded, perception that non-secretor milk is “inferior.” To address this untested hypothesis, we re-analyzed two datasets in which we previously showed that breastfeeding was protective against early life asthma and excessive infant weight gain in the Canadian CHILD Cohort Study. Using stratified regression models, we found that the protective association of exclusive breastfeeding and infant asthma was not modified by maternal secretor status (secretors aOR: 0.53, 95% CI 0.31 to 0.92; non-secretors aOR: 0.36, 95% CI 0.12 to 1.04; p for interaction = 0.50, N = 2086 children). Similarly, the association of breastfeeding with lower infant BMI and weight gain velocity did not vary by maternal secretor status (infant BMI: secretors aβ −0.47, 95% CI −0.66 to −0.29; non-secretors aβ −0.46, 95% CI −0.78 to −0.13; p for interaction = 0.60; N = 1971 infants). Our results indicate that secretor and non-secretor mothers can equally promote infant growth and respiratory health through breastfeeding. These findings run contrary to the idea that non-secretor milk is an inferior food source, and instead reify the importance of breastfeeding for all infants. The results of this study can inform feeding recommendations that are applicable to all infants, regardless of maternal secretor status.
Application of natural and modified additives in yogurt formulation: types, production, and rheological and nutraceutical benefits
Authors: Bankole Abigael Odunayo , Irondi Emmanuel Anyachukwu , Awoyale Wasiu , Ajani Emmanuel Oladipo
Published: October 2023

Yogurt, a popular fermented dairy product, is of different types and known for its nutritional and nutraceutical benefits. However, incorporating additives into yogurt has been adopted to improve its functionality and nutraceutical properties. Additives incorporated in yogurt may be natural or modified. The incorporation of diverse natural additives in yogurt formulation, such as moringa, date palm, grape seeds and argel leaf extracts, cornelian cherry paste, mulberry fruit and leaf powder, lentil flour, different types of fibers, lemongrass and spearmint essential oils, and honey, has been reported. Similarly, modified additives, such as β-glucan, pectin, inulin, sodium alginate, and gelatin, are also added to enhance the physicochemical, textural, sensory, and rheological properties of yogurt. Although additives are traditionally added for their technological impact on the yogurt, studies have shown that they influence the nutritional and nutraceutical properties of yogurt, when added. Hence, yogurts enriched with functional additives, especially natural additives, have been reported to possess an improved nutritional quality and impart several health benefits to consumers. These benefits include reducing the risk of cardiovascular disease, cancer, osteoporosis, oxidative stress, and hyperglycemia. This current review highlights the common types of yogurt, the production process, and the rheological and nutraceutical benefits of incorporating natural and modified additives into yogurt.
Acrofacial vitiligo secondary to PI3KCA inhibitor, alpelisib: case report
Authors: Hinich Maayan Geller , Hijab Adham
Published: October 2023

Alpelisib plus fulvestrant is a valid second or advanced line of treatment for patients with metastatic hormone receptor (HR)-positive, HER2-negative breast cancer who harbor an activating PIK3CA mutation. The well-known side effects of alpelisib are hyperglycemia, rash, and diarrhea. Herein, we report a case of a woman who developed diffuse depigmented macules on the face, arms and legs, three months after initiating alpelisib. Both clinical and histopathological findings were consistent with new-onset vitiligo. To our knowledge, this is the first case described in literature which suggests a causal relationship between alpelisib and irreversible dermatological adverse effect.
Effects of anisotropy and disorder on the superconducting properties of niobium
Authors: Zarea Mehdi , Ueki Hikaru , Sauls J. A.
Published: October 2023

We report results for the superconducting transition temperature and anisotropic energy gap for pure niobium based on Eliashberg’s equations and electron and phonon band structures computed from density functional theory. The electronic band structure is used to construct the Fermi surface and calculate the Fermi velocity at each point on the Fermi surface. The phonon bands are in excellent agreement with inelastic neutron scattering data. The corresponding phonon density of states and electron–phonon coupling define the electron–phonon spectral function, α2F(p, p′; ω), and the corresponding electron–phonon pairing interaction, which is the basis for computing the superconducting properties. The electron–phonon spectral function is in good agreement with existing tunneling spectroscopy data except for the spectral weight of the longitudinal phonon peak at ℏωLO = 23 meV. We obtain an electron–phonon coupling constant of λ = 1.057, renormalized Coulomb interaction μ⋆ = 0.218, and transition temperature Tc = 9.33 K. The corresponding strong-coupling gap at T = 0 is modestly enhanced, Δ0 = 1.55 meV, compared to the weak-coupling BCS value Δ0wc=1.78kBTc=1.43meV. The superconducting gap function exhibits substantial anisotropy on the Fermi surface. We analyze the distribution of gap anisotropy and compute the suppression of the superconducting transition temperature using a self-consistent T-matrix theory for quasiparticle-impurity scattering to describe niobium doped with non-magnetic impurities. We compare these results with experimental results on niobium SRF cavities doped with nitrogen impurities.
Comparison of different prediction models for estimation of walking and running energy expenditure based on a wristwear three-axis accelerometer
Authors: Xu Luyou , Zhang Jinxi , Li Zhen , Liu Yu , Jia Zhuang , Han Xiaowei , Liu Chenglin , Zhou Zhixiong
Published: October 2023

Objective: Objectively and efficiently measuring physical activity is a common issue facing the fields of medicine, public health, education, and sports worldwide. In response to the problem of low accuracy in predicting energy consumption during human motion using accelerometers, a prediction model for asynchronous energy consumption in the human body is established through various algorithms, and the accuracy of the model is evaluated. The optimal energy consumption prediction model is selected to provide theoretical reference for selecting reasonable algorithms to predict energy consumption during human motion.

Methods: A total of 100 subjects aged 18–30 years participated in the study. Experimental data for all subjects are randomly divided into the modeling group (n = 70) and validation group (n = 30). Each participant wore a triaxial accelerometer, COSMED Quark pulmonary function tester (Quark PFT), and heart rate band at the same time, and completed the tasks of walking (speed range: 2 km/h, 3 km/h, 4 km/h, 5 km/h, and 6 km/h) and running (speed range: 7 km/h, 8 km/h, and 9 km/h) sequentially. The prediction models were built using accelerometer data as the independent variable and the metabolic equivalents (METs) as the dependent variable. To calculate the prediction accuracy of the models, root mean square error (RMSE) and bias were used, and the consistency of each prediction model was evaluated based on Bland–Altman analysis.

Results: The linear equation, logarithmic equation, cubic equation, artificial neural network (ANN) model, and walking-and-running two-stage model were established. According to the validation results, our proposed walking-and-running two-stage model showed the smallest overall EE prediction error (RMSE = 0.76 METs, Bias = 0.02 METs) and the best performance in Bland–Altman analysis. Additionally, it had the lowest error in predicting EE during walking (RMSE = 0.66 METs, Bias = 0.03 METs) and running (RMSE = 0.90 METs, Bias < 0.01 METs) separately, as well as high accuracy in predicting EE at each single speed.

Conclusion: The ANN-based walking-and-running two-stage model established by separating walking and running can better estimate the walking and running EE, the improvement of energy consumption prediction accuracy will be conducive to more accurate to monitor the energy consumption of PA.
Global evaluation of key factors influencing nitrogen fertilization efficiency in wheat: a recent meta-analysis (2000-2022)
Authors: Yokamo Solomon , Irfan Muhammad , Huan Weiwei , Wang Bin , Wang Yiliu , Ishfaq Muhammad , Lu Diajun , Chen Xiaoqin , Cai Qiuliang , Wang Huoyan
Published: October 2023

Improving nitrogen use efficiency (NUE) without compromising yield remains a crucial agroecological challenge in theory and practice. Some meta-analyses conducted in recent years investigated the impact of nitrogen (N) fertilizer on crop yield and gaseous emissions, but most are region-specific and focused on N sources and application methods. However, various factors affecting yield and N fertilizer efficiency in wheat crops on a global scale are not extensively studied, thus highlighting the need for a comprehensive meta-analysis. Using 109 peer-reviewed research studies (published between 2000 and 2022) from 156 experimental sites (covering 36.8, 38.6 and 24.6% of coarse, medium, and fine texture soils, respectively), we conducted a global meta-analysis to elucidate suitable N management practices and the key factors influencing N fertilization efficiency in wheat as a function of yield and recovery efficiency and also explained future perspectives for efficient N management in wheat crop. Overall, N fertilization had a significant impact on wheat yield. A curvilinear relationship was found between N rates and grain yield, whereas maximum yield improvement was illustrated at 150-300 kg N ha-1. In addition, N increased yield by 92.18% under direct soil incorporation, 87.55% under combined chemical and organic fertilizers application, and 72.86% under split application. Site-specific covariates (climatic conditions and soil properties) had a pronounced impact on N fertilization efficiency. A significantly higher yield response was observed in regions with MAP > 800 mm, and where MAT remained < 15 °C. Additionally, the highest yield response was observed with initial AN, AP and AK concentrations at < 20, < 10 and 100-150 mg kg-1, respectively, and yield response considerably declined with increasing these threshold values. Nevertheless, regression analysis revealed a declining trend in N recovery efficiency (REN) and the addition of N in already fertile soils may affect plant uptake and RE. Global REN in wheat remained at 49.78% and followed a negative trend with the further increase of N supply and improvement in soil properties. Finally, an advanced N management approach such as “root zone targeted fertilization” is suggested to reduce fertilizer application rate and save time and labor costs while achieving high yield and NUE.
Enhancing ESL learner’s literacy by peer-assisted learning strategy of online English news
Authors: Gong Wen
Published: October 2023

As a result of globalization, countries are collaborating more and sharing more information. People’s perspectives now encompass both domestic and international news. In English as a Second Language (ESL) classes, the appropriate use of rich and vibrant online English news resources gives second language learners (L2 learners) direct access to first-hand, authentic English knowledge through independent reading and digestion, as well as peer-assisted classroom activities. Through weekly peer-assisted sharing of online English news, the goal of this study is to improve L2 learners’ literacy, global competence, and appreciation of cultural diversity. This study examines the participants’ perceptions and feedback using both quantitative and qualitative methods to confirm the efficacy of this pedagogy. After the semester-long experiment, significant differences were found in the English proficiency test. Particularly, the experimental class’s reading and writing scores were significantly higher (p < 0.05) than the control class’s, indicating that this pedagogy can effectively improve the literacy of ESL learners. The semi-structured interview revealed that participants were pleased with this instruction and perceived improvements in their language skills. ESL students’ core English literacy has significantly improved by expanding their comprehension of news articles and cultivating a sense of social responsibility.
The impact of a changed writing environment on students' motivation to write
Authors: Myhill Debra , Cremin Teresa , Oliver Lucy
Published: October 2023

Introduction

The act of writing is widely acknowledged to be a complex and challenging activity, and in parallel, we know that student motivation to write is a predictor of writing performance. So understanding what characteristics of the writing classroom support or foster motivation remains a salient concern. Research has shown that UK teachers are more likely to see themselves as readers than writers, which may affect how they teach writing.

Methods

This paper reports on student focus group interview data from a study which sought to strengthen teachers' sense of themselves as writers, and to examine the impact of this on students' classroom experience of writing and their writing outcomes. The participant teachers experienced a creative writing residential, which established a writing community led by two professional writers, with the goal of changing teachers' professional practice in their own writing classrooms. The study was mixed methods, comprising a randomized controlled trial and a comprehensive qualitative dataset collating data from both the residential and the classroom. This paper presents the qualitative analysis of 32 interviews with 16 student focus groups, exploring their responses to their teachers' changed practices and how it connected with their motivation to write.

Results

The interview analysis shows how many students responded positively to new teaching practices which gave them greater autonomy and choice, and established a more collaborative way of working. This led to increased confidence in and motivation to write.

Discussion

The study highlights the importance of the classroom environment in supporting and sustaining motivation to write, and underlines that motivation is not simply an internal characteristic of an individual but is situated within the context of a community of writers.
Recognition of masked and unmasked facial expressions in males and females and relations with mental wellness
Authors: Huc Marie , Bush Katie , Atias Gali , Berrigan Lindsay , Cox Sylvia , Jaworska Natalia
Published: October 2023

Background

While the effects of mask wearing/facial occlusion are known to impair facial expression recognition, little is known about the role of mental wellness on facial expression recognition, as well as the influence of sex on misattribution errors (i.e., confusions between emotions). In this large study, we aimed to address the relation between facial expression recognition and loneliness, perceived stress, anxiety, and depression symptoms in male and female adults.

Methods

We assessed the influence of mask-wearing on facial expression recognition [i.e., accuracy and response time (RT)] via an online study in N = 469 adult males and females across Canada.

Results

Expectedly, recognition was impaired under masked conditions (i.e., lower accuracy, longer RTs, more misattribution errors). Females were faster and more accurate than males, with less misattribution errors. A novel finding was that people with higher perceived stress were less accurate at identifying masked fearful faces. Perceived stress influenced the relation between sex and RT to masked happy faces; males with high stress scores were slower to recognize masked happy faces, the opposite was true for females. Finally, this study was among the first to show that higher loneliness predicted shorter RT to unmasked faces.

Impact

Our results show that facial expression recognition is impaired by mask-wearing, and that sex and mental health features are important predictors of performance. Such insight could be detrimental in certain sectors of the population (e.g., health care or education), and inform policies being adopted in future pandemics.
The landslide’s conceptualizing economic decline and its framing effect: Mandarin evidence
Authors: Zhang Yan , Yang Wenxing
Published: October 2023

Conceptual metaphors are essential for explaining and understanding social concerns. Natural disaster metaphors are commonly employed to access the abstract and negative impacts of social issues. Five of the top 10 most prevalent natural disaster frames in the Center for Chinese Linguistics (CCL)—earthquake, flood, fire hazard, drought, typhoon, landslide, volcano, sandstorm, tsunami, and debris flow—share a common economic target domain and show economic recession. Additionally, corpus-based research has revealed that the landslide frame is the most salient in figuratively representing economic declines. An experimental study derived from the corpus analysis has found that the landslide-framed economic crises posed more severity to participants and exerted a notable influence on their opinions and judgments. Therefore, when effective communication of economic hazards is to be realized, metaphorical representation of economic crises demands great consideration.
Spatial variation and determinants of inappropriate complementary feeding practice and its effect on the undernutrition of infants and young children aged 6 to 23 months in Ethiopia by using the Ethiopian Mini-demographic and health survey, 2019: spatial and multilevel analysis
Authors: Derseh Nebiyu Mekonnen , Shewaye Daniel Alayu , Agimas Muluken Chanie , Alemayehu Meron Asmamaw , Aragaw Fantu Mamo
Published: October 2023

Background

Inappropriate complementary feeding practices (IACFPs) are major public health issues in Ethiopia, which usually result in stunting and becoming an intergenerational cycle. However, the spatial patterns and determinants of IACFP and its effect on undernutrition are not well understood in Ethiopia. Therefore, the aim of this study was to explore the spatial patterns and determinants of IACFPs and their effects on the undernutrition of infants and young children (IYC) in Ethiopia.

Methods

This was a nationwide community-based survey study among 1,463 mothers of IYC aged 6–23 months in Ethiopia. The global spatial autocorrelation was assessed using the global Moran’s-I to evaluate the spatial clustering of IACFPs. Significant clusters with high and low rates of IACFPs were explored. A multilevel mixed-effect logistic regression with cluster-level random effects was fitted to identify determinants of IACFPs with an AOR and 95% CI.

Results

The prevalence of IACFPs was 90.22%. The spatial pattern of IACFP in Ethiopia was clustered across regions (Global Moran’s I = 0.63, Z-score = 12.77, value of p ≤0.001). Clusters with a high rate of IACFP were detected in southern, northwest, and eastern Ethiopia. Individual and community-level variables accounted for 63% of IACFP variation. Mothers with no education were 3.97 times (AOR = 3.97; 95% CI: 1.64–9.60) more likely to have IACFPs than those with higher education. The poorest HHs had 4.80 times the odds of having IACFPs as the richest HHs (AOR = 4.80, 95% CI: 1.23–18.71). The odds of having IACFPs were 2.18 times (AOR = 2.18; 95% CI: 1.28–3.72) higher among babies with no postnatal checkup. Non-breastfed IYC were 2.8 times (AOR = 2.80; 95% CI: 1.29–6.10) more likely to have IACFP when compared with breastfed ones. IYC with the inadequate introduction of solid, semi-solid, and soft foods, inadequate minimum dietary diversity, and inadequate minimum acceptable diet were more likely to have wasting, underweight, and stunting.

Conclusion

This study showed the prevalence of IACFPs was very high, which had an effect on undernutrition and showed spatial variation in Ethiopia. Therefore, the government of Ethiopia and stakeholders should focus on women with no education and the poorest HHs, encourage PNC checkups, and encourage breastfeeding in the hotspot areas to minimize IACFPs in Ethiopia.
Clinical outcome prediction of acute neurological patients admitted to the emergency department: Sequential Organ Failure Assessment score and modified SOFA score
Authors: Donoso-Calero María I. , Sanz-García Ancor , Polonio-López Begoña , Maestre Miquel Clara , Durantez Fernández Carlos , Mordillo-Mateos Laura , Mohedano-Moriano Alicia , Conty-Serrano Rosa , Otero-Agra Martin , Jorge-Soto Cristina , Martín-Conty José L. , Martín-Rodríguez Francisco
Published: October 2023

Background

The aim of this study was to determine the ability of the Sequential Organ Failure Assessment score (SOFA) and modified SOFA score (mSOFA) as predictive tools for 2-day and 28-day mortality and ICU admission in patients with acute neurological pathology treated in hospital emergency departments (EDs).

Methods

An observational, prospective cohort study in adults with acute neurological disease transferred by ambulance to an ED was conducted from 1 January 2019 to 31 August 2022 in five hospitals in Castilla-León (Spain). Score discrimination was assessed by the area under the curve (AUC) of the receiver operating characteristic (ROC) curve of the score.

Results

A total of 640 adult patients with neurological disease were included. For the prediction of 2-day mortality (all-cause), mSOFA presented a higher AUC than SOFA (mSOFA = 0.925 vs. SOFA = 0.902). This was not the case for 28-day mortality, for which SOFA was higher than mSOFA (mSOFA = 0.852 vs. SOFA = 0.875). Finally, ICU admission showed that SOFA was higher than mSOFA (mSOFA = 0.834 vs. SOFA = 0.845).

Conclusion

Both mSOFA and SOFA presented similar predictive ability, with mSOFA being the best predictor for short-term mortality and SOFA being the best predictor for medium-term mortality, as well as for ICU admission. These results in a cohort of patients with acute neurological pathology pave the way for the use of both predictive tools in the ED. The inclusion of these tools could improve the clinical assessment and further treatment of neurological patients, who commonly present the worst outcomes.
The impact of COVID-19 on sexual behavior, HIV prevention interest, general healthcare access, and other HIV risk factors among trial participants in Malawi, South Africa, Uganda, and Zimbabwe
Authors: Mancuso Noah , Mathebula Florence , Chitukuta Miria , Matambanadzo Kudzai V. , Tenza Siyanda , Reddy Krishnaveni , Nobula Lumka , Kemigisha Doreen , Stoner Marie C. D.
Published: October 2023

Introduction

The COVID-19 pandemic greatly impacted HIV prevention and care globally. The pandemic also had disproportionate impacts on the financial, emotional, and physical wellbeing of women and girls in East and Southern Africa, who were already at increased HIV vulnerability. This study aimed to understand how the COVID-19 pandemic and its response efforts impacted the sexual behavior, HIV prevention interest, general healthcare access, and other HIV risk factors of women and girls in HIV prevention studies.

Methods

Using the socio-ecological model (SEM), an explanatory sequential mixed-methods analysis was performed with data from four Microbicide Trial Network (MTN) studies on different populations—adolescent girls and young women (AGYW), pregnant persons, breastfeeding persons, and couples—in Malawi, South Africa, Uganda, and Zimbabwe. Descriptive statistics for outcomes of interest were calculated within each study separately and Chi-squared tests of independence were performed to evaluate associations between study population and outcomes. Excerpts from study qualitative interviews were stratified into code reports which were then summarized into memos with key themes and considerations of the SEM framework to provide context to quantitative findings.

Results

Few participants (8/731) had known or suspected COVID-19 infection. Sexual frequency and alcohol use decreased most often among AGYW compared to pregnant or breastfeeding women and couples (p-value < 0.001). The pandemic had little impact on changes in reported HIV prevention interest or access to HIV prevention study products. Healthcare access was impacted for everyone, with couples most likely to report decreases in access (p-value < 0.001). From qualitative interviews, economic instability, adverse mental health, and increased violence due to COVID-19 caused increased strain on other factors related to HIV vulnerability.

Conclusions

While interest in HIV prevention did not change and a few HIV risks decreased for most women and girls, other vulnerabilities to HIV increased due to the COVID-19 pandemic, highlighting the importance of continued access to HIV prevention for women and girls. More research is needed to better understand the long-term impact of COVID-19 on HIV prevention and vulnerability in community populations.
Fighting the hunger games through permaculture: a scoping review to inform urban planning
Authors: Janse van Rensburg Denise , Puren Karen
Published: October 2023

In 2020, more than seven million people across the globe starved, and 868 million people were estimated to be undernourished globally. Although the eradication of hunger is a noble goal, the continuously growing world population together with the warning of a future characterized by food insecurity spells ongoing hardship for the future of humankind. This study explores the extent of permaculture literature to establish its potential place within urban systems. A scoping review looks at permaculture literature from the last 8 years to establish its benefits and limitations, possibly within urban systems. Permaculture is mainly studied from social, environmental, and economic perspectives, and mostly within rural contexts. Urban planning directly concerns the social, environmental, and economic dimensions of urban systems. This study thus reveals the possible merits of permaculture for food production within urban systems; however, certain limitations should be kept in mind that may encumber the implementation of permaculture by urban planning.
Urban ecosystem services and climate change: a dynamic interplay
Authors: Pandey Bhanu , Ghosh Annesha
Published: October 2023

Urban ecosystems play a crucial role in providing a wide range of services to their inhabitants, and their functioning is deeply intertwined with the effects of climate change. The present review explores the dynamic interplay between urban ecosystem services and climate change, highlighting the reciprocal relationships, impacts, and adaptation strategies associated with these phenomena. The urban environment, with its built infrastructure, green spaces, and diverse human activities, offers various ecosystem services that enhance the wellbeing and resilience of urban dwellers. Urban ecosystems offer regulatory services like temperature control, air quality upkeep, and stormwater management, plus provisioning like food and water. They also provide cultural benefits, promoting recreation and community unity. However, climate change poses significant challenges to urban ecosystem services. Rising temperatures, altered precipitation patterns, and increased frequency of extreme weather events can disrupt the functioning of urban ecosystems, impacting the provision of services. Heatwaves and urban heat island effects can compromise human health and energy demands, while changes in rainfall patterns can strain stormwater management systems and lead to flooding. Moreover, climate change can disrupt biodiversity and ecological processes, affecting the overall resilience and sustainability of urban ecosystems. To address these challenges, cities are adopting various adaptation strategies that recognize the interdependence between urban ecosystems and climate change. Green infrastructure interventions, such as the creation of urban parks, green roofs, and community gardens, aim to mitigate the impacts of climate change by enhancing the regulation of temperature, improving air quality, and reducing stormwater runoff. Additionally, urban planning and design approaches prioritize compact and walkable neighborhoods, promoting public transportation and reducing reliance on fossil fuels. Furthermore, engaging communities in the management of urban ecosystems and climate change adaptation measures is crucial for ensuring equitable distribution of ecosystem services and building social resilience. Therefore, the review article highlights a comprehensive understanding of the dynamic interrelationship between urban ecosystem services and climate change and their implications. By recognizing and integrating the contributions of urban ecosystems, cities can develop sustainable and resilient strategies to mitigate and adapt to climate change, ensuring the wellbeing and habitability of urban environments for present and future generations.
Ultrasound-assisted fortification of yellow sweet potato (Ipomoea batatas) with iron and ascorbic acid
Authors: Purizaca-Santisteban Kateryn , Ruiz-Flores Luis Alberto , Sócola Zury , Chaves Eduardo Sidinei , Espinoza-Delgado Milagros del Pilar
Published: October 2023

The objective of this study was to evaluate the effect of ultrasound on the incorporation of iron and ascorbic acid (AA) in sweet potato (Ipomoea batatas) and to optimize the process parameters to obtain a fortified food. The incorporation was carried out using cubes of sweet potato submerged in 0.1% m/v ferrous sulfate and 1% m/v AA solutions, treated at different times and sonication frequencies (37 and 80 kHz), at 100 watts of power and 30 ± 5°C. ANOVA and Tukey’s test at 5% significance were applied to establish significant differences and the process was evaluated using a factorial design. The results revealed that the application of ultrasound influences the content of iron and AA, incorporating greater amount of iron and AA compared to samples not treated with ultrasound. Similarly, longer times led to higher incorporation of iron and AA content in sweet potatoes; the frequency was not statistically significant. The highest iron content was 105.91 ± 0.03 mg/100 g and for AA, it was 392.65 ± 4.84 mg AAE/100 g. The defined ultrasonic process conditions produced an increase of 4928.99 and 610.65%, respectively, in iron and AA content in sweet potato.
Application of a High-Density Single Nucleotide Polymorphism Genetic Map in Mapping Quantitative Trait Loci of Early-Maturing Traits in Upland Cotton
Authors: Mao Guangzhi , Wei Hengling , Chen Pengyun , Xing Feng , Wang Hantao
Published: October 2023

(1) Background: Mapping QTLs for early-maturing traits is necessary for the development of early-maturing variety breeding. (2) Methods: In this research, a high-density genetic map (HDGM) was constructed using an F2 population with 100 individuals and single nucleotide polymorphism markers (SNPs) developed using the genotyping-by-sequencing (GBS) method. (3) Results: The HDGM, which covered a total distance of 3167.14 cM, harbored 5454 SNPs with an average marker interval of 0.58 cM. In total, 18 QTLs for four early-maturing characters were detected and explained 11.6–46.4% of phenotypic variation (PV). Two QTLs of the whole growing period (WGP) and height of the node of the first fruiting branch (HNFFB) were identified as stable QTLs. In total, 125 candidate genes were identified in the confidence intervals of these stable QTLs. Presumably, Gh_D03G0857 may play an important role in regulating earliness. (4) Conclusions: This research will provide new information about fine mapping of QTLs for earliness traits, molecular marker assisted selection (MAS) of earliness traits, and pyramiding breeding as well.