bluuebunny/crossref_metadata_2025 huggingface dataset:
Radiomic Consensus Clustering in Glioblastoma and Association with Gene Expression Profiles
Authors: Wroblewski Tadeusz H. , Karabacak Mert , Seah Carina , Yong Raymund L. , Margetis Konstantinos
Published: December 2024

Background/Objectives: Glioblastoma (GBM) is the most common malignant primary central nervous system tumor with extremely poor prognosis and survival outcomes. Non-invasive methods like radiomic feature extraction, which assess sub-visual imaging features, provide a potentially powerful tool for distinguishing molecular profiles across groups of patients with GBM. Using consensus clustering of MRI-based radiomic features, this study aims to investigate differential gene expression profiles based on radiomic clusters. Methods: Patients from the TCGA and CPTAC datasets (n = 114) were included in this study. Radiomic features including T1, T1 with contrast, T2, and FLAIR MRI sequences were extracted using PyRadiomics. Selected radiomic features were then clustered using ConsensusClusterPlus (k-means base algorithm and Euclidean distance), which iteratively subsamples and clusters 80% of the data to identify stable clusters by calculating the frequency in which each patient is a member of a cluster across iterations. Gene expression data (available for n = 69 patients) was analyzed using differential gene expression (DEG) and gene set enrichment (GSEA) approaches, after batch correction using ComBat-seq. Results: Three distinct clusters were identified based on the relative consensus matrix and cumulative distribution plots (Cluster 1, n = 25; Cluster 2, n = 46; Cluster 3, n = 43). No significant differences in patient demographic characteristics, MGMT methylation status, tumor location, or overall survival were identified across clusters. Differentially expressed genes were identified in Cluster 1, which have been previously associated with GBM prognosis, recurrence, and treatment sensitivity. GSEA of Cluster 1 showed an enrichment of genes upregulated for immune-related and DNA metabolism pathways and genes downregulated in pathways associated with protein and histone deacetylation. Clusters 2 and 3 exhibited fewer DEGs which failed to reach significance after multiple testing corrections. Conclusions: Consensus clustering of radiomic features revealed unique gene expression profiles in the GBM cohort which likely represent subtle differences in tumor biology and radiosensitivity that are not visually discernible, underscoring the potential of radiomics to serve as a non-invasive alternative for identifying GBM molecular heterogeneity. Further investigation is still required to validate these findings and their clinical implications.
Relative Survival, Conditional Survival, and Causes of Death in Patients with Early Gastric Cancer, with a Focus on Differences Between Cardia and Non-Cardia Cancer
Authors: Elgenidy Anas , Alomari Omar , Hesn Mohamed Marey , Khaled Anas , Nada Sarah A. , Elsayed Mostafa , Mahmoud Ali , Al-kurdi Mohammed Al-mahdi , Afifi Ahmed M. , Cholankeril George
Published: December 2024

Background: Many researchers believe that cardia (CGC) and non-cardia (NCGC) are two different types of tumors, having different features like incidence rate, risk factors, geographical location, and socioeconomic status. This study aims to investigate the causes of death (COD) survival rates among early gastric cancer patients with a focus on differences between CGC and NCGC. Methods: This retrospective study employed SEER*stat software (version 8.3.92) to analyze the SEER 17 plus dataset (2000–2019). Standardized mortality ratios (SMR) were computed. Relative survival and conditional survival post-diagnosis were calculated using R software (version 4.1.0) among the different subgroups. Results: Within the follow-up period, 55.4% (5381) died, predominantly within the initial year post-diagnosis. Esophageal cancer was the leading non-gastric cancer cause in CGC, while miscellaneous tumors dominated in NCGC. The 1-year and 5-year relative survival for CGC patients were 76.4% and 48.9% respectively, while for NCGC were 80.4% and 63.9%. The 3-year conditional survival after 1 year and 5e years of survival for CGC were 68.7% and 88.8%, respectively, while for NCGC were 82.2% and 93.5%, respectively. This means that the longer a person has survived after diagnosis with cancer, the greater the likelihood that person will survive for another 3 years. Conclusions: This study sheds light on the substantial impact of non-cancer COD in GC patients, underscoring the necessity of considering comorbidities in their comprehensive management and follow-up. Impact: This study contributes valuable insights for clinical decision-making and informs future research directions regarding CGC and NCGC.
Targeting of Non-Classical Human Leukocyte Antigens as Novel Therapeutic Strategies in Cancer
Authors: Benitez Fuentes Javier David , Bartolome Arcilla Jorge , Mohamed Mohamed Kauzar , Lopez de Sa Alfonso , de Luna Aguilar Alicia , Guevara-Hoyer Kissy , Ballestin Martinez Pablo , Lazaro Sanchez Antonio David , Carosella Edgardo D. , Ocaña Alberto , Sánchez-Ramon Silvia
Published: December 2024

Human leukocyte antigens (HLAs) are essential regulators of immune responses against cancer, with classical HLAs well-documented for their role in tumor recognition and immune surveillance. In recent years, non-classical HLAs—including HLA-E, HLA-F, HLA-G, and HLA-H—have emerged as critical players in the immune landscape of cancer due to their diverse and less conventional functions in immune modulation. These molecules exhibit unique mechanisms that enable tumors to escape immune detection, promote tumor progression, and contribute to therapeutic resistance. This review provides a comprehensive examination of the current understanding of non-classical HLAs in solid cancers, focusing on their specific roles in shaping the tumor microenvironment and influencing immune responses. By analyzing how HLA-E, HLA-F, HLA-G, and HLA-H modulate interactions with immune cells, such as T cells, natural killer cells, and antigen-presenting cells, we highlight key pathways through which these molecules contribute to immune evasion and metastasis. Additionally, we review promising therapeutic strategies aimed at targeting non-classical HLAs, including emerging immunotherapies that could potentially enhance cancer treatment outcomes by reversing immune suppression within tumors. Understanding the influence of these non-classical HLAs in solid cancers may offer new insights into cancer immunology and may lead to the development of innovative and more effective immunotherapeutic approaches. This review underscores the importance of non-classical HLAs as potential therapeutic targets, providing a necessary foundation for future studies in the evolving field of cancer immunotherapy.
Per-Irradiation Monitoring by kV-2D Acquisitions in Stereotactic Treatment of Spinal and Non-Spinal Bony Metastases Using an On-Board Imager of a Linear Accelerator
Authors: Hadj Henni Ahmed , Martinage Geoffrey , Lebret Lucie , Arhoun Ilias
Published: December 2024

Background/Objectives: An on-board imager on a linear accelerator allows the acquisition of kV-2D images during irradiation. Overlaying specific structures on these images enables the visual verification of movement at regular frequencies. Our aim was to validate this tracking method for the stereotactic treatment of bone metastases. Methods: Shifts in three translational directions were simulated using an anthropomorphic phantom. For these simulated shifts, planar images were acquired at different angles of incidence, with overlaid volumes of interest. A blinded test was then administered to the 18 participants to evaluate their decisions regarding whether to stop treatment. The results considered the experience of the operators. Quantitative analyses were performed on the intra-fractional images of 29 patients. Results: Participants analyzed each image with an average (standard deviation) decision time of 3.0 s (2.3). For offsets of 0.0, 1.0, 1.5, and 2.0 mm, the results were 78%, 93%, 90%, and 100% for the expert group and 78%, 70%, 79%, and 88% for the less-experienced group. Clinical feedback confirmed this guidance technique and extended it to non-spinal bony metastases. Sudden movements exceeding the 2.0 mm threshold occurred in 3.3% of the analyzed fractions, with a detection rate of 97.8% for vertebral locations. For non-vertebral bone locations, movements exceeding a threshold of 3.0 mm occurred in 3.5% of cases and were detected in 96.5%. Conclusions: The clinical use of planar OBI and superimposed structures for visual-image guidance in bone stereotactic treatment was validated using an anthropomorphic phantom and clinical feedback.
Disrupted Lipid Metabolism, Cytokine Signaling, and Dormancy: Hallmarks of Doxorubicin-Resistant Triple-Negative Breast Cancer Models
Authors: Vishnubalaji Radhakrishnan , Alajez Nehad M.
Published: December 2024

Background: Chemoresistance in triple-negative breast cancer (TNBC) presents a significant clinical hurdle, limiting the efficacy of treatments like doxorubicin. This study aimed to explore the molecular changes associated with doxorubicin resistance and identify potential therapeutic targets to overcome this resistance, thereby improving treatment outcomes for TNBC patients. Methods: Doxorubicin-resistant (DoxR) TNBC models (MDA-MB-231 and BT-549) were generated by exposing cells to increasing concentrations of doxorubicin. RNA sequencing (RNA-Seq) was performed using the Illumina platform, followed by bioinformatics analysis with CLC Genomics Workbench and iDEP. Functional assays assessed proliferation, sphere formation, migration, and cell cycle changes. Protein expression and phosphorylation were confirmed via Western blotting. Pathway and network analyses were conducted using Ingenuity Pathway Analysis (IPA) and STRING, while survival analysis was performed using Kaplan–Meier Plotter database. Results: DoxR cells exhibited reduced proliferation, sphere formation, and migration, but showed enhanced tolerance to doxorubicin. Increased CHK2 and p53 phosphorylation indicated cellular dormancy as a resistance mechanism. RNA-Seq analysis revealed upregulation of cytokine signaling and stress-response pathways, while cholesterol and lipid biosynthesis were suppressed. Activation of the IL1β cytokine network was prominent in DoxR cells, and CRISPR-Cas9 screens data identified dependencies on genes involved in rRNA biogenesis and metabolism. A 27-gene signature associated with doxorubicin resistance was linked to worse clinical outcomes in a large breast cancer cohort (HR = 1.76, FDR p < 2.0 × 10−13). Conclusions: This study uncovers potential therapeutic strategies for overcoming TNBC resistance, including dormancy reversal and targeting onco-ribosomal pathways and cytokine signaling networks, to improve the efficacy of doxorubicin-based treatments.
Effectiveness of the Korean National Cancer Screening Program in Reducing Colorectal Cancer Mortality
Authors: Lee Hyeon Ji , Lee Kyeongmin , Kim Byung Chang , Jun Jae Kwan , Choi Kui Son , Suh Mina
Published: December 2024

Background: Whether colorectal cancer (CRC) screening with a fecal immunochemical test (FIT) reduces mortality remains unclear. In South Korea, CRC screening with a FIT for individuals aged ≥ 50 years has been part of the Korean National Cancer Screening Program (KNCSP) since 2004. The aim of this study was to evaluate the effectiveness of the KNCSP in reducing CRC-specific mortality. Methods: We conducted a nested case-control study using cohort-based data derived from the KNCSP database. The cohort included 5,944,540 colorectal cancer-free individuals aged ≥ 50 years as of 2004. Individuals who died after CRC diagnosis were defined as cases (n = 29,992) and their sociodemographic characteristics were matched to those of the selected controls. The effects of screening exposure, frequency, and time interval on CRC-specific mortality were analyzed according to age group. Conditional logistic regression analysis was performed. Results: Compared with individuals who had never been screened, those who had ever been screened showed an OR of 0.74 (95% CI, 0.71–0.76) for CRC-specific mortality. CRC-specific mortality decreased as the number of screenings increased. Similar results were observed for those aged 50–79 years; however, the results for those aged 75–79 years were not statistically significant. Moreover, those aged ≥ 80 years had the opposite results. Conclusions: CRC mass screening using FIT is effective for individuals aged 50–74 years; therefore, this study suggests that countries considering introducing national CRC screening implement FIT for those within this age range.
Partial Inhibition of Epithelial-to-Mesenchymal Transition (EMT) Phenotypes by Placenta-Derived DBMSCs in Human Breast Cancer Cell Lines, In Vitro
Authors: Basmaeil Yasser , Subayyil Abdullah Al , Kulayb Haya Bin , Kondkar Altaf A. , Alrodayyan Maha , Khatlani Tanvir
Published: December 2024

Stem cell-based therapies hold significant potential for cancer treatment due to their unique properties, including migration toward tumor niche, secretion of bioactive molecules, and immunosuppression. Mesenchymal stem cells (MSCs) from adult tissues can inhibit tumor progression, angiogenesis, and apoptosis of cancer cells. We have previously reported the isolation and characterization of placenta-derived decidua basalis mesenchymal stem cells (DBMSCs), which demonstrated higher levels of pro-migratory and anti-apoptotic genes, indicating potential anti-cancer effects. In this study, we analyzed the anti-cancer effects of DBMSCs on human breast cancer cell lines MDA231 and MCF7, with MCF 10A used as control. We also investigated how these cancer cells lines affect the functional competence of DBMSCs. By co-culturing DBMSCs with cancer cells, we analyzed changes in functions of both cell types, as well as alterations in their genomic and proteomic profile. Our results showed that treatment with DBMSCs significantly reduced the functionality of MDA231 and MCF7 cells, while MCF 10A cells remained unaffected. DBMSC treatment decreased epithelial-to-mesenchymal transition (EMT)-related protein levels in MDA231 cells and modulated expression of other cancer-related genes in MDA231 and MCF7 cells. Although cancer cells reduced DBMSC proliferation, they increased their expression of anti-apoptotic genes. These findings suggest that DBMSCs can inhibit EMT-related proteins and reduce the invasive characteristics of MDA231 and MCF7 breast cancer cells, highlighting their potential as candidates for cell-based cancer therapies.
The Application of Molecularly Imprinted Polymers in Forensic Toxicology: Issues and Perspectives
Authors: Mohamed Susan , Santelli Simone , Giorgetti Arianna , Pelletti Guido , Pirani Filippo , Fais Paolo , Pascali Jennifer P.
Published: December 2024

Molecularly imprinted polymers (MIPs) are synthetic receptors designed to selectively bind specific molecules, mimicking natural antibody–antigen interactions. Produced through polymerization around a target molecule (template), MIPs create imprints that confer high specificity and binding affinity upon template removal. Initially developed in the 1970s with organic polymers, MIPs now play critical roles in separation sciences, catalysis, drug delivery, and sensor technology. In forensic science, MIPs offer potential for sample preparation, pre-concentration, and analyte detection, especially with complex biological and non-biological matrices. They exhibit superior stability under extreme conditions, enabling their use in challenging forensic contexts such as detecting new psychoactive substances or trace explosives. Despite advantages like reusability and high selectivity, MIPs face limitations in forensic analysis due to their complex synthesis, potential template leakage, and non-specific binding. Moreover, the lack of standardized protocols limits their mainstream adoption, as forensic applications require validated, reproducible methods. This review systematically assesses MIPs in forensic toxicology, focusing on their current capabilities, limitations, and potential for broader integration into forensic workflows. Future research should address standardization and evaluate MIPs’ effectiveness in diverse forensic applications to realize their full potential.
Cholelithiasis in Infants: Risk Factors, Management, and the Role of Ursodeoxycholic Acid
Authors: Çakar Sevim , Eren Gülin , Erdur Cahit Barış , Önder Mehmet , Pelek Şafak , Alpdoğan Sedef , Demirtaş Duygu , Ecevit Çiğdem Ömür , Bekem Özlem
Published: December 2024

Background: Cholelithiasis is a rare disease in infants, and there is limited data on its risk factors and management. Objectives: To evaluate the risk factors, management, and response to medical treatment of cholelithiasis in infants. Methods: Infants diagnosed with cholelithiasis by ultrasound between 2018 and 2023 were retrospectively analyzed. Details of patient history, imaging findings, current symptoms, and treatments were reviewed. Results: Over 5 years, 98 infants were diagnosed with cholelithiasis. Thirty-three (33.7%) were girls, and the most common risk factors were the use of cephalosporin antibiotic therapy in 46.9%, sepsis in 30.6%, total parenteral nutrition in 29.6%, prematurity in 27.6%, congenital heart disease in 18.4%, and genetic disease (Down syndrome diagnosis in seven patients) in 16.3%. Only fifteen patients (15.3%) were symptomatic. Ursodeoxycholic acid (UDCA) treatment was given to 90.8% of patients, but nine of them used it for a short period or irregularly, and regular users were 81.6%. Gallstones disappeared in 46 patients (46.9%), including 14 (30.4%) without using UDCA regularly. The response rate to UDCA treatment was lower in preterm infants (p = 0.004). Gallstone resolution was higher in the nonusers, 14/18 (77.8%) versus 32/79 (40.5%) (p = 0.03). Acute cholecystitis was observed in only four patients; no other complications were noted. No infant required surgical or endoscopic treatment. Conclusions: UDCA should not be used routinely in children, especially infants, except in symptomatic children with a contraindication to surgery or to reduce clinical symptoms. In the absence of symptoms, patients may be monitored clinically.
The Impact of an External Compared to an Internal Focus of Attention on Simple and Complex Motor Skills Acquisition in Novice Female Volleyball Players
Authors: Lola Afrodite , Dalamitros Athanasios A. , Zafeiroudi Aglaia , Eradli Nur , Clemente-Suárez Vicente Javier , Martín-Rodríguez Alexandra
Published: December 2024

Objectives: This study aimed to investigate the effects of external (EXT) versus internal (INT) focus of attention on acquiring and transferring simple and complex motor skills in novice female volleyball players. Methods: Fifty-seven participants were assigned to one of the three groups: The external focus (EXT), the internal focus (INT), and the control group (CON). Over a 6-week intervention, participants practiced a simple skill (volleyball passing) and a complex skill (overhead tennis serve). Pre-tests, post-tests, and transfer tests (conducted two weeks later) assessed movement form and accuracy. Results: The results showed a statistically significant interaction among focus methods, skill complexity, and testing periods for movement form and performance outcome. The EXT group showed higher movement form and performance outcome improvements during the simple skill (passing) compared to the INT and CON groups. For the complex skill (serving), the EXT group outperformed the INT and CON groups regarding movement outcome; however, no differences in movement form were observed between the EXT and INT groups. Post hoc Tukey tests indicated that the EXT group scored higher than the INT and CON groups in the post-test and transfer test for the simple skill, with movement form improving from 1.68 ± 0.48 to 2.32 ± 0.58. In the complex skill, the EXT group’s movement outcome improved from 11.32 ± 6.83 to 44.47 ± 16.57. Conclusions: External focus significantly enhances movement form and outcome in simple tasks and performance outcomes in more complex skills among novice athletes.
Swiss Consensus on Prenatal and Early Postnatal Urinary Tract Dilation: Practical Approach and When to Refer
Authors: Bahadori Atessa , Wilhelm-Bals Alexandra , Caccia Julien , Chehade Hassib , Goischke Alexandra , Habre Céline , Marx-Berger Daniela , Nef Samuel , Sanchez Oliver , Spartà Giuseppina , Vidal Isabelle , von Vigier Rodo O. , Birraux Jacques , Parvex Paloma
Published: December 2024

Urinary tract dilations (UTDs) are the most frequent prenatal renal anomaly. The spectrum of etiologies causing UTD ranges from mild spontaneously resolving obstruction to severe upper and lower urinary tract obstruction or reflux. The early recognition and management of these anomalies allows for improved renal endowment prenatally and ultimately better outcome for the child. The role of the general obstetrician and pediatrician is to recognize potential prenatal and postnatal cases addressed to their practice and to refer patients to specialized pediatric nephrology and urology centers with a sense of the urgency of such a referral. The aim of this paper is to offer clinical recommendations to clinicians regarding the management of neonates and children born with prenatally detected UTD, based on a consensus between Swiss pediatric nephrology centers. The aim is to give suggestions and recommendations based on the currently available literature regarding classifications and definitions of prenatal and postnatal UTD, etiologies, prenatal and postnatal renal function evaluation, investigations, antibiotic prophylaxis, and the need for referral to a pediatric nephrologist and/or urologist. The overarching goal of a systematic approach to UTD is to ultimately optimize kidney health during childhood and improve long-term renal function prognosis.
Atomization Performance of Spray Nozzles and Their Influence on Fine Particle Collection in the Wet Electrostatic Precipitator
Authors: Li Hengtian , Xiao Lichun , Wang Shuting , Ren Gaijuan
Published: December 2024

The wet electrostatic precipitator (WESP) is crucial for the ultra-purification of blast furnace gas in gas-fired generator units. To address issues like high water consumption, poor atomization leading to spark discharge, and uneven water mist distribution, a water mist testing system using a laser particle-size analyzer was established. Eight spray nozzles were tested to identify the optimal atomization performance and operating parameters. The effect of chemical agglomeration agents on nozzle atomization and particle capture efficiency was also examined. The results show that the atomization effect was the best when the operating water pressure was 0.5 MPa. The D50 of the blast furnace dust increased from 8.529 μm to 20.30 μm after electrostatic precipitation when the 1/8 rotating core nozzles were installed in the WESP, and the proportion of dust particles whose diameter is ≤5 μm decreased by 20.09% compared with the dust emitted from the inlet. The total dust removal efficiency reached 83.41%. With chemical agglomeration, the D50 reached 24.88 μm, and removal efficiency rose to 96.98%. Among the tested nozzles, the 1/8 rotating core nozzle was the most effective, combining superior atomization, maximum dust removal efficiency, and minimal water consumption, making it ideal for blast furnace gas purification.
An Experimental Study Based on the Surface Microstructure of Bionic Marine Animals
Authors: Chen Chaoda , Yu Zhuoyuan , Shao Xiaoqiang , Zou Baojian , Xu Biaoqing , Xiao Zeping , Tang Zhenyu
Published: December 2024

Masked jet electrolytic machining has the advantages of high machining efficiency and good surface morphology, giving it important applications in fields such as bionic marine animal manufacturing. The factors affecting the electrolytic machining speed are deduced; a modeling simulation is carried out by COMSOL software; the electrolyte potential map and current density line map inside the microgroove are analyzed; and measurements of the actual machined microgroove are made by a scanning microscope to carry out the experiments of electrolytic characteristics and morphology of the microgroove under different pulse voltages, machining gaps, and machining times. Experiments show that the pulse voltage plays a dominant role in processing, and when the pulse voltage is increased from 50 V to 125 V, the microgroove width increases by an average of 7.7%, and the depth increases by an average of 28.8%, which significantly improves the surface microstructure of the bionic marine animal.
Carbon-Negative Nano-TiO2-Modified Photocatalytic Cementitious Composites: Removal of Airborne Pollutants (NOx and O3) and Its Impact on CO2 Footprint
Authors: Kalinowski Maciej , Chilmon Karol , Jackiewicz-Rek Wioletta
Published: December 2024

This study explores the development and performance of photocatalytic cementitious composites modified with nano-TiO2 to address urban air quality and sustainability challenges. Nine mortar series were prepared, incorporating binders with varying carbon footprints and mass contents across different series. The interplay between the fundamental (abrasion resistance) and functional (air purification efficiency) properties of the composites’ surfaces and interfaces was investigated. The photocatalytic removal of airborne pollutants, specifically nitrogen oxides (NOx) and ozone (O3), was evaluated under simulated environmental conditions. The variations in binder composition influenced the composites’ overall initial carbon footprint and air purification efficiency. The assessment revealed a possible net decrease in carbon emissions over the life cycle of the composite due to the removal of ozone (greenhouse gas) and its precursor—NOx, highlighting the potential of photocatalytic cementitious composites for dual environmental benefits in an urban environment, emphasizing the critical role of surface and interface engineering in achieving carbon-negative composites.
Microstructure and Corrosion Resistance of Laser-Cladded FeCo1.5CrNi1.5Ti0.5 High-Entropy Alloy Coatings
Authors: Wang Sui , Tian Siqi , Liu Renjie , Chen Dengya , Wang Chao , Li Jing , Yang Sen
Published: December 2024

Due to their excellent mechanical properties and corrosion resistance, high-entropy alloys (HEAs) have the potential to be used as new engineering structures and functional materials. In this study, an FeCo1.5CrNi1.5Ti0.5HEA coating was prepared on the surface of a 1Cr18Ni9Ti alloy by laser cladding technology. Phase structure and microstructure were characterized by XRD and using an SEM. The corrosion resistance was evaluated by an electrochemical workstation, and the polarization curves were obtained in simulated seawater and 3.5 wt.% NaCl and 5% HCl solutions. The corrosion morphology of the Fe-based HEA coating was further characterized using the SEM, super depth of field observation, and 3D topological images. The results showed that the Fe-based HEA coating had a single-phase FCC structure with a grain size of about 10.7 ± 0.25 μM. Electrochemical analysis results showed that the corrosion resistance of the current Fe-based HEA coating was poor in HCl solutions. However, it exhibited good corrosion properties in simulated seawater and 3.5 wt.% NaCl solutions. Further analysis of the corrosion morphology revealed that in simulated seawater and the 3.5 wt.% NaCl solution, the surface of the current Fe-based HEA coating exhibited a preferential corrosion tendency between dendrites, while in the 5% HCl solution, it exhibited more obvious pitting characteristics. The results indicate that the current Fe-based HEA coating exhibits good comprehensive performance, especially in an acidic Cl− corrosion environment. These findings provide a reference for the application of laser cladding prepared Fe HEA coatings.
Abrasive and Erosive Wear Behavior of Elastomeric Polyurethane Coatings: Effect of Grain Size
Authors: Sokolska Justyna , Ptak Anita
Published: December 2024

Polyurethane coatings are widely used as protective layers against wear, mainly abrasive wear. They have recently been applied to surfaces exposed to erosive wear, such as wind turbine blades. This study investigated the abrasive and erosive wear of polyurethane elastomeric coatings with hardness values of 55 ShA, 75 ShA, and 95 ShA. The abrasive wear test was carried out using loose abrasive grains. The erosive wear test was carried out using a pressurized stream of gas containing abrasive particles. Both tests were carried out using aluminum oxide grains of five different sizes to evaluate the effect of particle size on wear behavior. Microscopic and profilometric analyses of the surface of the wear tracks were carried out. The mechanism of abrasive and erosive wear of polyurethane elastomeric coatings was determined. The results of the tests show a non-linear dependence of abrasive and erosive wear on the grain size. Furthermore, polyurethane elastomer coatings with a higher hardness exhibit a lower abrasive wear resistance but higher susceptibility to erosive wear. These findings provide insight into the trade-offs between hardness and wear performance, offering practical guidance for selecting polyurethane coatings in applications requiring resistance to combined abrasive and erosive wear.
Experimental and Simulation Studies on Thermal Shock of Multilayer Thermal Barrier Coatings with an Intermediate Transition Layer at 1500 °C
Authors: Liu Pengpeng , Yang Shilong , Li Kaibin , Wang Weize , Liu Yangguang , Yang Ting
Published: December 2024

Strain tolerance is a crucial factor affecting the thermal life of coatings, and a higher strain tolerance can effectively alleviate the thermal stresses on coatings during thermal shock. To improve the strain tolerance, the coating structure was optimized by introducing an intermediate transition layer in this study. The intermediate transition layer material was prepared using a 1:1 volume ratio mixture of 6–8 wt. % Yttria-stabilized zirconia (YSZ) and NiCrAlY powders in the experiments. The coating structure consisted of an Al2O3-GdAlO3 (AGAP) anti-erosion layer, a YSZ layer, an intermediate transition layer, and a bonding layer from top to bottom. After thermal shock experiments at 1500 °C, the coatings with the addition of the intermediate transition layer exhibited different failure modes, with the crack location shifting from between the YSZ and the bonding layer to within the intermediate transition layer, compared to the coatings without the intermediate transition layer. Finite element simulation analysis showed that the intermediate transition layer effectively increased the strain tolerance of the coating and significantly reduced the thermal stress. Furthermore, incorporating an embedded micron agglomerated particle-based (EMAP) thermal barrier coating structure into the intermediate transition layer effectively alleviated thermal stresses and enhanced the coating’s thermal insulation performance.
Effect of Ion-Assisted Deposition Energy of RF Source on Optical Properties, Microstructure, and Residual Stress of HfO2 Thin Films
Authors: Wang Bo , Zhang Jian , Liu Hai , Yang Haigui , Wang Yanchao , Wang Haifeng , Pan Jingjie , Liu Zhen , Shen Zhenfeng , Gao Wenkai , Hu Hanwen , Zhao Yi , Tang Haolong , Wang Tongtong , Wang Xiaoyi
Published: December 2024

HfO2 thin films were prepared using radio frequency (RF) ion source-assisted deposition, and the effects of auxiliary ion energy on the microstructure, optical properties, and residual stress of the films were systematically studied. The experimental results showed that when the auxiliary ion energy increased, the extinction coefficient, compressive stress, and optical band gap were gradually increased. These changes were attributed to increased grain boundary defects, crystal structure disorder, and grain size decrease due to high-energy ion bombardment. The HfO2 films deposited at a lower ion energy (600 V) exhibited higher surface quality (RMS = 0.78 nm), better optical properties (k = 10⁻5), and lower residual stress (1.26 GPa).
Enhancing Accessibility: Automated Tactile Graphics Generation for Individuals with Visual Impairments
Authors: Dzhurynskyi Yehor , Mayik Volodymyr , Mayik Lyudmyla
Published: December 2024

This study addresses the accessibility challenges faced by individuals with visual impairments due to limited access to graphic information, which significantly impacts their educational and social integration. Traditional methods for producing tactile graphics are labor-intensive and require specialized expertise, limiting their availability. Recent advancements in generative models, such as GANs, diffusion models, and VAEs, offer potential solutions to automate the creation of tactile images. In this work, we propose a novel generative model conditioned on text prompts, integrating a Bidirectional and Auto-Regressive Transformer (BART) and Vector Quantized Variational Auto-Encoder (VQ-VAE). This model transforms textual descriptions into tactile graphics, addressing key requirements for legibility and accessibility. The model’s performance was evaluated using cross-entropy, perplexity, mean square error, and CLIP Score metrics, demonstrating its ability to generate high-quality, customizable tactile images. Testing with educational and rehabilitation institutions confirmed the practicality and efficiency of the system, which significantly reduces production time and requires minimal operator expertise. The proposed approach enhances the production of inclusive educational materials, enabling improved access to quality education and fostering greater independence for individuals with visual impairments. Future research will focus on expanding the training dataset and refining the model for complex scenarios.
Research on the Modulation Characteristics of LiNbO3 Crystals Based on the Three-Dimensional Ray Tracing Method
Authors: Sun Houpeng , Li Yingchun , Zhang Laixian , Guo Huichao , Luan Chenglong , Zheng Haijing , Li Rong , Fan Youchen
Published: December 2024

To further study the electro-optical modulation characteristics of LiNbO3 crystals and analyze their modulation performance, a method for studying the modulation characteristics of LiNbO3 crystals, based on the three-dimensional ray tracing method, is introduced. With the help of the refractive index ellipsoidal theory, the optical properties of LiNbO3 crystals under the influence of the Pockels effect are systematically investigated. The research results show that the optical properties of LiNbO3 crystals under the action of an external electric field can be divided into two cases: the crystal optical axis is parallel to the clear light direction, and the crystal optical axis is perpendicular to the clear light direction. Subsequently, starting from Maxwell’s equation and the matter equation, the analytical expressions of optical parameters such as refractive index, wave vector, light vector, optical path, and phase delay in electro-optical crystals are derived. Finally, the propagation law of LiNbO3 crystals when the light is incident in any direction, i.e., when the optical axis of the crystal is parallel to the clear direction and perpendicular to the clear direction, and the light intensity and field of view of the LiNbO3 crystal for electro-optical modulation are discussed.
Mechanism of Aluminium Electrochemical Oxidation and Alumina Deposition Using a Carbon Sphere Electrode
Authors: Yang Shumin , Wang Ao , Lin Xuemei , Qi Yunkai , Shi Guochao , Han Wei , Gu Jianjun
Published: December 2024

Using electromagnetic and electrochemical theories as a framework, this study examines the influence of carbon sphere electrodes on the distribution patterns of anodic oxidation and deposition current densities in metallic aluminium and porous anodic alumina. Theoretical calculations show that the current density symmetrically decreases from the centre outward under the effect of carbon sphere electrodes. Increasing the electrode distance improves the uniformity of the current distribution across the film, while decreasing the distance increases the rate of gradient change in current density. Simulation results reveal that at electrode spacings of 15 cm and 1 cm, the oxidation current density at the film centre is 1333 A/m2 and 2.9 × 105 A/m2, respectively. The current density gradually decreases outward along the radius, reaching 1330 A/m2 and 1.8 × 105 A/m2 at the edges, with observed current density gradient change rates of 500 A/m3 and 1.83 × 107 A/m3, respectively. Experimental results confirm that carbon sphere counter electrodes can create non-uniform oxidation and deposition electric fields. Microstructures with gradually varying symmetry can be generated by adjusting the electrode spacing, resulting in porous anodic alumina and composite films exhibiting iridescent, ring-like structural colours. The experimental findings align well with theoretical calculations and simulation results.
Axisymmetric Free Vibration of Functionally Graded Piezoelectric Circular Plates
Authors: Li Yang , Gao Yang
Published: December 2024

An analytical solution is presented for axisymmetric free vibration analysis of a functionally graded piezoelectric circular plate on the basis of the three-dimensional elastic theory of piezoelectric materials. The material properties are assumed to follow an exponential law distribution through the thickness of the circular plate. The state space equations for the free vibration behavior of the functionally graded piezoelectric circular plate are developed based on the state space method. The finite Hankel transform is utilized to obtain an ordinary differential equation with variable coefficients. By virtue of the proposed exponential law model, we have ordinary differential equations with constant coefficients. Then, the free vibration behaviors of the functionally graded piezoelectric circular plate with two kinds of boundary conditions are investigated. Some numerical examples are given to validate the accuracy and stability of the present model. The influences of the exponential factor and thickness-to-span ratio on the natural frequency of the functionally graded piezoelectric circular plate, constrained by different boundary conditions, are discussed in detail.
From Tradition to Innovation: Diverse Molecular Techniques in the Fight Against Infectious Diseases
Authors: Alsharksi Ahmed Nouri , Sirekbasan Serhat , Gürkök-Tan Tuğba , Mustapha Adam
Published: December 2024

Infectious diseases impose a significant burden on global health systems due to high morbidity and mortality rates. According to the World Health Organization, millions die from infectious diseases annually, often due to delays in accurate diagnosis. Traditional diagnostic methods in clinical microbiology, primarily culture-based techniques, are time-consuming and may fail with hard-to-culture pathogens. Molecular biology advancements, notably the polymerase chain reaction (PCR), have revolutionized infectious disease diagnostics by allowing rapid and sensitive detection of pathogens’ genetic material. PCR has become the gold standard for many infections, particularly highlighted during the COVID-19 pandemic. Following PCR, next-generation sequencing (NGS) has emerged, enabling comprehensive genomic analysis of pathogens, thus facilitating the detection of new strains and antibiotic resistance tracking. Innovative approaches like CRISPR technology are also enhancing diagnostic precision by identifying specific DNA/RNA sequences. However, the implementation of these methods faces challenges, particularly in low- and middle-income countries due to infrastructural and financial constraints. This review will explore the role of molecular diagnostic methods in infectious disease diagnosis, comparing their advantages and limitations, with a focus on PCR and NGS technologies and their future potential.
A New Method of Predicting Final Mandibular Length Based on the Morphology of Cervical Vertebrae
Authors: Yamaguchi Manami , Kim Yong-Il , Park Heetae , Yamaguchi Tetsutaro
Published: December 2024

Background/Objectives: Methods for predicting final mandibular length have been studied for many years. We aimed to estimate the final mandibular length at the end of the growth period by analyzing changes in cervical vertebral morphology using longitudinal lateral cephalograms. Methods: Longitudinal lateral cephalograms of elementary school students aged 6–15 who did not undergo orthodontic treatment, collected between 1965 and 1973, were used. For this analysis, 370 images from 44 female individuals were selected, and cervical vertebral morphology was assessed using the semi-landmark method. Generalized Procrustes analysis and principal component analysis were performed, and changes in maxillofacial skeletal and cervical vertebral morphology were analyzed using a linear mixed model with repeated measures. A predictive formula for estimating final mandibular length was developed based on morphological changes in the fourth cervical vertebra. Results: The difference between the predicted and actual final mandibular lengths using the semi-landmark method was 0.17 ± 0.08 mm. The marginal R2 value of the prediction formula was 0.957, and the conditional R2 was 0.990, demonstrating very high accuracy. The annual increase in mandibular length remained consistent each year but slowed after 14 years of age. Geometric morphometric analysis revealed significant morphological changes in the fourth cervical vertebra at 14 years of age, coinciding with a deceleration in mandibular growth. Conclusions: A highly accurate formula was developed to predict final mandibular length based on morphological changes in the fourth cervical vertebra. Cervical vertebral morphology may provide valuable information related to mandibular growth during adolescence.
The Challenge of Deep Learning for the Prevention and Automatic Diagnosis of Breast Cancer: A Systematic Review
Authors: Pérez-Núñez Jhelly-Reynaluz , Rodríguez Ciro , Vásquez-Serpa Luis-Javier , Navarro Carlos
Published: December 2024

Objectives: This review aims to evaluate several convolutional neural network (CNN) models applied to breast cancer detection, to identify and categorize CNN variants in recent studies, and to analyze their specific strengths, limitations, and challenges. Methods: Using PRISMA methodology, this review examines studies that focus on deep learning techniques, specifically CNN, for breast cancer detection. Inclusion criteria encompassed studies from the past five years, with duplicates and those unrelated to breast cancer excluded. A total of 62 articles from the IEEE, SCOPUS, and PubMed databases were analyzed, exploring CNN architectures and their applicability in detecting this pathology. Results: The review found that CNN models with advanced architecture and greater depth exhibit high accuracy and sensitivity in image processing and feature extraction for breast cancer detection. CNN variants that integrate transfer learning proved particularly effective, allowing the use of pre-trained models with less training data required. However, challenges include the need for large, labeled datasets and significant computational resources. Conclusions: CNNs represent a promising tool in breast cancer detection, although future research should aim to create models that are more resource-efficient and maintain accuracy while reducing data requirements, thus improving clinical applicability.
High-Sensitivity Flow Cytometry for the Reliable Detection of Measurable Residual Disease in Hematological Malignancies in Clinical Laboratories
Authors: Álvarez Flores María Beatriz , Sopeña Corvinos María , Guillén Santos Raquel , Cava Valenciano Fernando
Published: December 2024

Background: Monitoring of measurable residual disease (MRD) requires highly sensitive flow cytometry protocols to provide an accurate prediction of shorter progression-free survival. High assay sensitivity generally requires rapid processing to avoid cell loss from small bone marrow sample volumes, but this requirement conflicts with the need in most clinical cytometry laboratories for long processing and acquisition times, especially when multiple MRD studies coincide on the same day. Methods: The proposed protocol was applied to 226 human bone marrow and 45 peripheral blood samples submitted for the study of MRD or the detection of rare cells. Samples were processed within 24 h of extraction and acquired with an eight-color flow cytometer. Results: The FACSLyse-Bulk protocol allows for the labelling of millions of cells in under 90 min in small sample volumes without affecting the FSC/SSC pattern or antigen expression, and it also allows antigens to be fixed to the membrane, thus avoiding the capping phenomenon. Conclusions: The proposed protocol would allow clinical flow cytometry laboratories to perform MRD studies in house and easily achieve a limit of detection and limit of quantification <0.001%, thus avoiding the need to outsource analysis to specialized cytometry laboratories.
The Efficacy and Safety of Ferric Carboxymaltose in Heart Failure with Reduced Ejection Fraction and Iron Deficiency: An Updated Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Padda Inderbir , Sebastian Sneha Annie , Fabian Daniel , Sethi Yashendra , Johal Gurpreet
Published: December 2024

Background: Iron deficiency (ID) often coexists with heart failure (HF), and its prevalence increases with the severity of HF. Intravenous ferric carboxymaltose (FCM) has been associated with improvements in clinical outcomes, functional capacity, and quality of life (QoL) in patients with HF and ID. However, while earlier studies showed favorable results, more recent studies have failed to demonstrate significant improvements in outcomes for patients with heart failure with reduced ejection fraction (HFrEF) and ID. This meta-analysis seeks to provide updated insights into the effectiveness and safety of FCM compared to placebo/standard of care (SoC) among patients with HFrEF and ID/iron deficiency anemia (IDA). Methods: We performed a systematic review and meta-analysis of the literature from inception to December 2023, utilizing databases such as MEDLINE (via PubMed), Google Scholar, the Cochrane Library, ClinicalTrials.gov, and the ScienceDirect portal. A statistical analysis was carried out using RevMan 5.4 with a random-effects model. Dichotomous outcomes were reported as odds ratios (OR), while continuous outcomes were presented as the weighted mean difference (WMD) with corresponding 95% confidence intervals (CI), and heterogeneity was assessed using the I2 test. Results: The final analysis included data from six randomized controlled trials (RCTs), comprising 5132 patients. Our findings indicate a significant reduction in total HF hospitalizations among patients with HFrEF and ID/IDA treated with FCM compared to those receiving the placebo or SoC, with an OR of 0.59 (95% CI: 0.40 to 0.88, p < 0.010). However, no statistically significant difference was observed in the total number of deaths between the FCM and placebo/SoC groups (OR: 0.85; 95% CI: 0.70 to 1.03, p = 0.09), non-HF hospitalizations (OR: 0.71; 95% CI: 0.41 to 1.25, p = 0.24), or the composite outcome of cardiovascular hospitalizations and cardiovascular deaths (OR: 0.65; 95% CI: 0.40 to 1.04, p = 0.07). Regarding functional capacity, as assessed by the change in 6-min walk test (6MWT) distance, no significant improvement was found, with a weighted mean difference (WMD) of 14.03 (95% CI: −10.94 to 38.99, p = 0.27). QoL, measured by the Kansas City Cardiomyopathy Questionnaire (KCCQ) score, also did not show significant enhancement, with a WMD of 3.85 (95% CI: −0.55 to 8.24, p = 0.09). Furthermore, the safety analysis revealed no significant difference in the incidence of serious adverse events between the FCM and placebo/SoC groups, with an OR of 0.73 (95% CI: 0.49 to 1.10, p = 0.13). Conclusions: In patients with HFrEF and IDA, treatment with intravenous FCM significantly lowers the risk of total HF hospitalizations but does not appear to affect functional capacity, QoL, or mortality.