bluuebunny/crossref_metadata_2025 huggingface dataset:
Preservation vs. Dissection of Inferior Pulmonary Ligament for Thoracoscopic Upper Lobectomy: A Prospective Randomized Controlled Trial
Authors: Qian Jiekun , Cai Shixian , Lin Pinghua , Chi Wanzhong , Chen Chun , Xu Guobin , Xu Chi , Wu Weidong , Zheng Wei , Zheng Bin
Published: July 2023

OBJECTIVES
The proper procedure for inferior pulmonary ligament (IPL) during upper lobectomy remains a topic of debate. To investigate this issue, we conducted an open, multicenter prospective randomized controlled trial comparing the clinical effects of preserving IPL versus dissecting IPL during thoracoscopic upper lobectomy.
METHODS
Patients undergoing thoracoscopic left/right upper lobectomy were assigned to either the dissection group (Group D) or the preserved group (Group P). Our primary purpose lay in measuring and contrasting the change of postoperative residual bronchial angle change, postoperative lung volume change, and other intraoperative and postoperative outcomes between the two groups.
RESULTS
Our findings revealed that in thoracoscopic left upper lobectomy, Group P was able to reduce the degree of postoperative residual bronchial angle change at 3 and 6 months after surgery, as compared to Group D, with a significant difference (P < 0.05). IPL preservation during thoracoscopic left upper lobectomy can therefore reduce the movement of the left residual bronchus.
CONCLUSIONS
Our study suggests that it makes no sense to dissect IPL since its dissection is of no benefit. These findings have important implications for the clinical management of patients undergoing upper lobectomy.
Parents’ Self-Compassion as a Moderator Between Children’s Emotional Regulation Difficulties and Parents’ Depressive Symptoms
Authors: Larrucea-Iruretagoyena Maite , Orue Izaskun , Calvete Esther
Published: July 2023

Background: Recent studies suggest that children’s emotional difficulties may impact their parents’ psychological well-being, and certain protective factors could mitigate this association. The existing literature points to self-compassion as a potential protective factor against stress and difficulties. Therefore, the present study aims to analyze the protective role of parents’ self-compassion in the predictive association between children’s emotional regulation difficulties and parents’ depressive symptoms.
Methods: A total of 214 children (53.3% girls) and their parents (209 mothers and 181 fathers) answered self-report questionnaires in a two-wave longitudinal study conducted over one year. A multilevel analysis was performed to examine the predictive pathways from children’s emotional regulation difficulties, parents’ self-compassion, and their interaction to parents’ depressive symptoms. Gender differences were addressed in the predictive associations.
Results:Children’s emotional regulation difficulties predicted their parents’ depressive symptoms after one year, and this association was reduced by the parents’ self-compassion. No significant gender differences were found for depressive symptoms or self-compassion, and parents’ gender did not moderate any of the longitudinal associations.
Limitations: The study exhibited a small sample and a lack of cultural and socioeconomic diversity.
Conclusion: Children’s emotional regulation difficulties may predict a worsening of their parents’ depressive symptoms, but self-compassion mitigates this relationship.
A SACS deletion variant in Great Pyrenees dogs causes autosomal recessive neuronal degeneration
Authors: Ekenstedt Kari J , Minor Katie M , Shelton G Diane , Hammond James J , Miller Andrew D , Taylor Susan M , Huang Yanyun , Mickelson James R
Published: July 2023

ARSACS (autosomal recessive spastic ataxia of Charlevoix-Saguenay) is a human neurological disorder characterized by progressive cerebellar ataxia and peripheral neuropathy. A recently recognized disorder in Great Pyrenees dogs is similarly characterized by widespread central nervous system degeneration leading to progressive cerebellar ataxia and spasticity, combined with peripheral neuropathy. Onset of clinical signs occurred in puppies as young as four months of age, with slow progression over several years. A multi-generation pedigree suggested an autosomal recessive mode of inheritance. Histopathology revealed consistent cerebellar Purkinje cell degeneration, neuronal degeneration in brainstem nuclei, widespread spinal cord white matter degeneration, ganglion cell degeneration, inappropriately thin myelin sheaths or fully demyelinated peripheral nerve fibers, and normal or only mild patterns of denervation atrophy in skeletal muscles. Genome-wide single nucleotide polymorphism (SNP) genotype data was collected from 6 cases and 26 controls, where homozygosity mapping identified a 3.3Mb region on CFA25 in which all cases were homozygous and all controls were either heterozygous or homozygous for alternate haplotypes. This region tagged the SACS gene where variants are known to cause ARSACS. Sanger sequencing of SACS in affected dogs identified a 4bp deletion that causes a frame shift and truncates 343 amino acids from the C terminus of the encoded sacsin protein (p.Val4244AlafsTer32). Our clinical and histopathological descriptions of this canine disorder contribute significantly to the description of human ARSACS and represents the first naturally-occurring large animal model of this disorder.
Mur Ligase F as a New Target for the Flavonoids Quercitrin, Myricetin, and (-)-Epicatechin
Authors: Hrast Martina , Zdovc Irena , Glavač Nina Kočevar , Gobec Stanislav , Frlan Rok
Published: July 2023

MurC, D, E, and F are ATP-dependent ligases involved in the stepwise assembly of the tetrapeptide stem of forming peptidoglycan. As highly conserved targets found exclusively in bacterial cells, they are of significant interest for antibacterial drug discovery. In this study, we employed a computer-aided molecular design approach to identify potential inhibitors of MurF. A biochemical inhibition assay was conducted, screening twenty-four flavonoids and related compounds against MurC-F, resulting in the identification of quercitrin, myricetin, and (-)-epicatechin as MurF inhibitors with IC50 values of 143 µM, 139 µM, and 92 µM, respectively. Notably, (-)-epicatechin demonstrated mixed type inhibition with ATP and uncompetitive inhibition with D-Ala-D-Ala dipeptide and UM3DAP substrates. Furthermore, in silico analysis using Sitemap and subsequent docking analysis using Glide revealed two plausible binding sites for (-)-epicatechin. The study also investigated the crucial structural features required for activity, with a particular focus on the substitution pattern and hydroxyl group positions, which were found to be important for the activity. The study highlights the significance of computational approaches in targeting essential enzymes involved in bacterial peptidoglycan synthesis.
Genetically predict diet-derived Antioxidants and risk of Neurodegenerative diseases among individuals of European descent: a Mendelian randomization study
Authors: Duan Qing-Qing , Su Wei-Ming , Gu Xiao-Jing , Long Jiang , Jiang Zheng , Yin Kang-Fu , Cai Wei-Chen , Cao Bei , Chi Li-Yi , Gao Xia , Chen Yong-Ping , Li Ju-Rong
Published: July 2023

Background
The prevalence of neurodegenerative disorders (NDDs) is increasing, and there is no cure for any of these diseases. Antioxidants have been associated with several diseases in observational studies. However, the relationship between antioxidants and neurodegenerative diseases remains unclear. The purpose of this study is to use publicly available genome-wide association studies (GWAS) summary statistics to examine the causal relationship between diet-derived antioxidants and the risk of NDDs.
Methods
Single-nucleotide polymorphisms associated with diet-derived antioxidants (vitamin A, vitamin B6, vitamin C, vitamin E, carotene, β-carotene, lycopene, zinc, and selenium) and their metabolites (α-tocopherol, γ-tocopherol, ascorbate, and retinol) were employed as instrumental variables. The NDDs we studied included Parkinson's disease (PD), Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and frontotemporal dementia (FTD), which were obtained from GWASs conducted in the European population. Two-sample Mendelian randomization (MR) was performed together with a series of sensitivity analyses. The main statistical analyses were conducted using package “TwoSampleMR (V.0.5.6)” in R (V.4.2.0)
Results
Genetically predicted α-tocopherol led to a lower risk of ALS (OR 0.45; 95% CI, 0.31, 0.66; p = 3.97E-05) based on the random-effect IVW method, and no evidence of heterogeneity and horizontal pleiotropy was found. In addition, we found a potential protective effect of vitamin E and ascorbate on PD, carotene on ALS, and retinol on FTD. However, no circulating antioxidants studied affect the risk for AD.
Conclusion
This study indicated that some diet-derived antioxidants or their metabolites showed evidence of causality in NDDs and provided promising targets for therapeutics.
Postoperative change in the joint-line convergence angle contributes to inaccurate correction in high tibial osteotomy
Authors: Kim Young , Joo Yong Bum , Park Young Cheol , Song Ju-Ho
Published: July 2023

Objective
Accurate correction is a prerequisite for the favorable outcomes of open-wedge high tibial osteotomy (OWHTO). However, previous studies have reported disappointing results regarding correction accuracy despite the use of intra-operative navigation, which implies that a certain factor other than bony components is involved in the inaccurate correction (mainly overcorrection). The joint-line convergence angle (JLCA) can represent soft tissue effects in OWHTO. This study tried to determine whether the postoperative change in the JLCA (∆JLCA) led to inaccurate correction.
Methods
Medical records of 78 OWHTO patients from 2005 to 2021 were retrospectively reviewed. The hip-knee-ankle angle (HKA) was measured with a positive value indicating varus alignment. Inaccurate correction was defined as |postoperative HKA| >3°. The JLCA was measured before and 6 months after surgery on long-standing hip-to-ankle radiographs, and ∆JLCA was defined as the difference between the preoperative and 6-month postoperative JLCAs. ∆JLCA was compared between the accurate correction group and the inaccurate correction group, and a receiver operating characteristic (ROC) curve was used to obtain the cutoff ∆JLCA at which the sensitivity and the specificity for inaccurate correction were maximized. Clinical outcomes were also compared between the groups using the knee injury and osteoarthritis outcome score (KOOS).
Results
Of the 78 patients, inaccurate correction was noted in 10 patients. The overall preoperative and postoperative HKAs were 7.0 ± 3.1° and − 0.4 ± 1.5°, respectively. The accurate correction group and the inaccurate correction group had a difference in ∆JLCA (p = 0.010). However, no significant difference was found in the preoperative HKA (p = 0.529). An ROC curve showed that the cutoff ∆JLCA was 1.9°. In the patients having ∆JLCA ≥ 1.9°, the mean JLCA was 4.9 ± 1.6° preoperatively and 1.7 ± 1.2° postoperatively. In the other patients having ∆JLCA < 1.9°, the mean preoperative and postoperative JLCA were 2.5 ± 1.8° and 2.3 ± 1.8°, respectively. The difference in the preoperative JLCA was significant (p < 0.001). The postoperative KOOS subscales did not differ according to correction accuracy.
Conclusion
Inaccurate correction in OWHTO, specifically valgus overcorrection, is attributable to ∆JLCA which represents the postoperative change of soft tissue effects. Overcorrection should be checked in cases of large preoperative JLCAs.
Absorption changes in Photosystem II in the Soret band region upon the formation of the chlorophyll cation radical [P D1 P D2 ] +
Authors: Boussac Alain , Sugiura Miwa , Nakamura Makoto , Nagao Ryo , Noguchi Takumi , Viola Stefania , Rutherford A. William , Sellés Julien
Published: July 2023

Flash-induced absorption changes in the Soret region arising from the [PD1PD2]+ state, the chlorophyll cation radical formed upon excitation of Photosystem II (PSII), were obtained using Mn-depleted PSII cores at pH 8.6. Under these conditions, TyrD is i) reduced before the first flash, and ii) oxidized before subsequent flashes. In wild-type PSII, when TyrD● is present, an additional signal in the [PD1PD2]+-minus-[PD1PD2] difference spectrum was observed when compared to the first flash when TyrD is not oxidized. The additional feature was “W-shaped” with troughs at 434 nm and 446 nm. This feature was absent when TyrD was reduced, but was present i) when TyrD was physically absent (and replaced by phenylalanine) or ii) when its H-bonding histidine (D2-His190) was physically absent (replaced by a Leucine). Thus, the simple difference spectrum without the double trough feature at 434 nm and 446 nm, seemed to require the native structural environment around the reduced TyrD and its H bonding partners to be present. We found no evidence of involvement of PD1, ChlD1, PheD1, PheD2, TyrZ, and the Cytb559 heme in the W-shaped difference spectrum. However, and surprisingly, the use of a mutant of the PD2 axial His ligand, the D2-His197Ala, shows that the PD2 environment seems involved in the “W-shaped” signal.
m6A reader YTHDF1 inhibits the growth of neuroblastoma in vitro and in vivo
Authors: Deng Jing , Long Jun , Yang Yang , Yang Fengyu
Published: July 2023

Purpose Neuroblastoma (NB) is the most common extracranial solid tumor in childhood, always leading to an unfavorable prognosis. m6A is the most prevalent modification in eukaryotic RNAs. Util now, the role of N6-methyladenosine (m6A) in NB has not been elucidated. In this study, we aim to explore the role of m6A reader YTHDF1 in regulating NB proliferation and growth in vitro and in vivo.Methods We evaluate the role of YTHDF1 in mediating NB cell growth using in vitro and in vivo methods. We perform immunohistochemistry assay to detect the expression of YTHDF1 in NB clinical samples. Cell proliferation assay was used to test the growth of NB cell line. The tumor xenograft mouse model was performed to evaluate the role of YTHDF1 in vivo. RNA-seq was applied to uncover the genes that changed upon YTHDF1 knockdown.Results Our results showed that the expression of YTHDF1 is significantly increased in clinical neuroblastoma samples compared with that of ganglioneuroma (GN) and ganglioneuroblastoma (GNB). Cellular assays demonstrated that knockdown of YTHDF1 inhibits NB cell growth and proliferation. The tumor xenograft mouse model suggests that knockdown of YTHDF1 suppresses the growth of neuroblastoma in vivo. RNA-seq showed that the expression of a series genes was changed upon YTHDF1 knockdown, including ACER3, WNT2B and ANKRD62.Conclusion In conclusion, our study provides a new therapeutic target for the treatment of neuroblastoma via inhibiting YTHDF1.
Reduction of Musculoskeletal Pain in First Postgraduate Year (PGY1) Medicine Residents: A Randomized Controlled Trial on Improving Work Life with Laptop Ergonomics
Authors: Oh Gary Chee Seng , Le Dat , Abugattas Alonso Daniel , Gautam Vivek , Espiritu Neil
Published: July 2023

Background
Work-related musculoskeletal disorders (WRMSDs) are a widely recognized problem among healthcare professionals. First postgraduate year (PGY1) students are particularly at risk of developing WRMSDs due to the demanding nature of their work. This study aimed to evaluate the effectiveness of an ergonomic intervention in reducing musculoskeletal pain among PGY1 and improving their work life.
Methods
This randomized control trial included participants from incoming first-year residents for the academic year 2022–2023. Exclusion criteria included daily medications for headaches, having a history of neck/spine pain injections, history of chronic pain, or already using laptop stands. Data collection utilized a Google Survey form incorporating pain scales at baseline, 3, and 6 months.
Results
Out of 57 potential candidates, 41 residents were eligible. Four subjects were lost due to dropout, transfer, or leaving the residency program, leaving 37 residents by the end. Significant differences (p < 0.05) among control and intervention are shown in shoulder pain, lower back pain, taller subjects with wrist/hand pain, and males with upper back pain. Trends (p < 0.10) existed with heavier subjects, males with upper back and wrist pain and taller subjects with headaches. The other differences in average pain scoring among different treatments, genders, weights, and heights was not statistically significant.
Conclusion
The study findings suggest that the intervention was effective in reducing shoulder, wrist, and lower back pain among residents. However, further research is needed to explore these outcomes.
Bone health in children with Angelman Syndrome at the ENCORE Expertise Center
Authors: Heus Karen G.C.B. Bindels-de , Hagenaar Doesjka A. , Mous Sabine E. , Dekker Ilonka , Kaay Daniëlle C.M. , Kerkhof Gerthe F. , Elgersma Ype , Moll Henriette A. , Wit Marie-Claire Y.
Published: July 2023

Purpose
Angelman Syndrome (AS) is a rare genetic disorder due to lack of UBE3A function on chromosome 15q11.2q13 caused by a deletion, uniparental paternal disomy (UPD), imprinting center disorder (ICD) or pathological variant of the UBE3A gene. AS is characterized by developmental delay, epilepsy, and lack of speech. Although fractures are reported frequently in clinical practice, there are few studies on bone health in AS. The aim of this study is to investigate bone health in children with AS.
Methods
Prospective cohort study of 91 children with AS visiting the ENCORE Expertise Center for AS between April 2010 and December 2021. Bone health was assessed with the Bone Health Index (BHI) in standard deviation score (SDS) measured by digital radiogrammetry of the left hand using BoneXpert software. Risk factors analyzed were age, sex, genetic subtype, epilepsy, anti-seizure medication (ASM) use, mobility, BMI, and onset of puberty.
Results
Children with AS had a mean BHI of -1.77 SDS (SD 1.4). A significantly lower BHI was found in children with a deletion (-2.24 SDS) versus non-deletion (-1.02 SDS). Other factors associated with reduced BHI-SDS were inability to walk and late onset of puberty. Children with a history of one or more fractures (22%) had a significantly lower BHI than children without fractures (-2.60 vs -1.56 SDS). Longitudinal analysis showed a significant decrease in BHI-SDS with age in all genetic subtypes.
Conclusions
Children with AS have a reduced bone health. Risk factors are deletion genotype, no independent walking, and late onset of puberty. Bone health decreased significantly with age.
Towards Controlled Semi-Autonomous Deconstruction
Authors: Lee Hyung Joo , Brell-Cokcan Sigrid
Published: July 2023

The automation of deconstruction processes presents unique difficulties due to the harsh working conditions involved. In this research, we aim to address these limitations by advancing the teleoperated machine, BROKK 170, towards a developed semi-autonomous concept. We propose a framework for robot-assisted deconstruction, exploring the communication and sensing systems of the prototype deconstruction robot, along with its capabilities. Additionally, field tests are conducted to evaluate the performance of the proposed approach in real-world scenarios. The potentials and limitations drawn from these initial results are discussed.
The Unsteady Full Multi-wake Vortex Lattice Method: a full rolled-up detached vorticity approach
Authors: Pimentel-Garcia Jesus Carlos
Published: July 2023

After numerically demonstrating that vorticity is generated on the entire surface for flow past a shell-body through the steady Full Multi-wake Vortex Lattice Method (FMVLM), two unsteady versions are developed. The first one is a straightforward approach, based on the well-known single trailing edge wake Unsteady Vortex Lattice Method (UVLM), but applied to multiple detached wakes, useful during the verification phase; the second one is an improved version of the original one, which enhances results for multi-wake dynamics and aerodynamic coefficients calculations, used during the validation phase. The obtained results allow ensuring that the Unsteady Full Multi-wake Vortex Lattice Method (UFVLM) is well-implemented from a strictly numerical viewpoint and constitutes the cornerstone to future unsteady developments, not only for ideal flow simulations, but also to solve more complex fluid dynamics from a purely Lagrangian description (e.g., through panel-vortex methods).
Synthesis of an Experimental Gel Containing Biologically Synthesized Silver Nanoparticles used in the Biofilm Disruption
Authors: Antunes Karina Eleonara Klein Graupen Roldan , Rocha Daniel Navarro , Marcelino Larissa , Silva Roberto do Nascimento , Nogueira Ana Paula , Facury Analia Gabriella Borges Ferraz , Costa Ana Rosa , Neves José Guilherme
Published: July 2023

The aim of the present study was to synthesize and evaluate the biological and mechanical properties of a polymeric gel associated with silver nanoparticles. The gels were composed of a Chitosan-Xanthan (CX) polyelectrolytic biopolymer complex associated or not with silver nanoparticles (Agnano), which were grouped into the following groups: G1) Gel CX; G2) CX Gel + 5% Agnano; G3) CX Gel + 2.5% Agnano; 4) CX Gel + 1.25% Agnano. The characterization of nanoparticles was performed through dynamic light scattering analysis, and gels through rheological analysis. The antimicrobial activity for Streptococcus mutans was performed by the inhibition halo method. The results obtained from DLS showed a Zeta potential of -30 mV and particle size approximately 100 nm. The rheological analysis shows that all groups had viscosity between 5 and 6.5 Pa, and with increasing shear stress the viscosity of all groups was decreased. For the analysis of antimicrobial activity, the antimicrobial potential was observed only for the CX gel containing 5% Agnano. Thus, it is concluded that the gel containing 5% Agnano is promising for use in orthodontic patients with a high rate of bacterial plaque and can be used to prevent white spot lesions during and after treatment.
Young practitioners membership evaluation: A qualitative exploration
Authors: Thomas James
Published: July 2023

The overarching objectives for this research were to explore the perceptions of newly minted bovine veterinarians regarding the American Association of Bovine Practitioners (the Associa­tion), determine its salient features and benefits, and ascertain if the Association has a non-renewal issue with this population.

This was the first investigation of this sort conducted by the As­sociation with this audience regarding these issues – the area in question was basically unexplored. The information previously available was mostly anecdotal, therefore In-Depth Interviews (IDIs), a qualitative research methodology was employed. A total of 19 IDIs were conducted with bovine veterinarians who had practiced for less than 5 years and had been a member of the American Association of Bovine Practitioners. Thirteen in­dividuals were current members, while 6 respondents were no longer members.

Although there were a number of suggestions for improvement, all respondents pointed out the many positive attributes of AABP and would recommend membership.
Feedlot telemedicine
Authors: Jim G. Kee
Published: July 2023

Feedlot Health Management Services by TELUS Agriculture and Consumer Goods was founded in 1983. The company’s cli­ent base as well as the scope, scale, and quality of services of­fered by FHMS has grown exponentially thanks to the early and ongoing adoption of technologies now collectively referred to telemedicine. The integration of remote diagnoses with near real-time individual health and production record keeping soft­ware allows veterinary consultants to provide ongoing surveil­lance and oversight in client operations.
Managing a business that has a practice within it
Authors: Gnad David P.
Published: July 2023

Mixed and large animal practices are diverse businesses influ­enced by the species mix, customer base, practitioner interests and focus among other things. This session will look at outside influences and internal practices that can affect a mixed/large animal practice. It will also explore synergistic or complimen­tary services and products that may complement the practice.
Common emergency and musculoskeletal repairs in the small ruminant
Authors: Muir Andrew
Published: July 2023

Small ruminants suffer musculoskeletal disease most com­monly due to fracture, wildlife attack, fence injuries, infec­tious or degenerative diseases. Due to their size, behavior and physiologic healing capacity compared to other species, they are excellent orthopedic patients. External coaptation is well tolerated in these species and can be applied in many cases, and often results in a satisfactory clinical outcome. In unfor­tunate circumstances where limb amputation is elected, small ruminants appear to tolerate this procedure and can have fair to good long-term outcomes.
Troubleshooting reproductive failures in small ruminants
Authors: Estill Charles T.
Published: July 2023

As a service to our commercial clients, and with the popularity of “urban agriculture”, large and mixed animal practitioners are frequently called upon to evaluate and treat reproductive disorders of small ruminants. Often, these animals are owned by clients with no or very little previous exposure to animal ag­riculture. In many cases, it is the practitioner’s goal to simply educate these neophyte owners on normal reproductive physi­ology and basic animal husbandry.

Both sheep and goats are inherently very fertile when infec­tious diseases are controlled, proper nutrition provided, and environmental extremes mitigated. Both species typically re­produce very efficiently with little human intervention. Howev­er, as practitioners, we are called upon to investigate reproduc­tive maladies that may be commonplace or, especially as “pet” sheep and goats are kept well past traditional reproductive ages, disorders with which we may be unfamiliar.

This presentation will use a problem list as the basis for dis­cussion of how to troubleshoot a wide range of reproductive disorders of small ruminants. Because of the large depth and breadth of disorders one may encounter, a full discussion of each malady and its treatment is beyond the scope of this pre­sentation. Rather, a series of common client complaints will be listed followed by a discussion of a systematic approach to establishing a diagnosis or etiology. The specifics of treatment will not be discussed.
Randomized clinical trial evaluating the effects of the administration of acidogenic boluses at dry off on udder health
Authors: Florentino C. , Mosca Peña , Ruch M. , Tikofsky L. , Knauer W. , Cramer G. , Godden S. , Caixeta L.
Published: July 2023

High milk production at dry off is a risk factor for intramam­mary infections (IMI) postpartum due to milk leakage and im­paired formation of the keratin plug during the dry period. The use of acidifying agents at dry off can induce a temporary feed intake reduction and decrease in milk yield of high-producing dairy cows. Thus, acidogenic boluses are a potential tool to help maintain udder health around the dry period. The study aim was to evaluate the effects of the administration of acidogenic boluses at dry off on udder health.
Quantification of antimicrobial usage on dairy farms before, during and after the implementation of farmworker stewardship training
Authors: Portillo-Gonzalez R. , Garzon-Audor A. , Pereira R. V. V. , Silva-del-Rio N. , Karle B. M. , Habing G. G.
Published: July 2023

Antimicrobials are critical to preserving animal health and welfare. However, the development of antimicrobial resistance represents a public health threat. Veterinarians prescribe an­timicrobials, but farmworkers are responsible for making on-farm treatment decisions and their training is vital to promote responsible antimicrobial use (AMU). This research project aimed to describe and quantify AMU on large dairy farms in Ohio and California and evaluate the impact of farmworker antimicrobial stewardship (AMS) training on those metrics. We hypothesized that farms, where AMS training was adminis­tered, would have significantly lower AMU compared to farms where training was not administered.
Evaluating factors affecting recovery of Mannheimia haemolytica and Pasteurella multocida
Authors: Garzon A. , Hoyos Jaramillo A. , Hustad S. , Byrne B. A. , Fritz H. M. , Pereira R.
Published: July 2023

Microbiological diagnosis is an important step in controlling and preventing bovine respiratory disease (BRD). Moreover, adequate transport storage type, elapsed time, and storage tem­perature before laboratory submission are critical for optimal results. The objective was to evaluate the effect of transport storage media, time and storage temperature on Mannheimia haemolytica (MH) and Pasteurella multocida (PM) yield using an in-vitro model simulation.
Investigation of the relationship between manure processing method and presence of M. avium subsp. paratuberculosis and Salmonella spp. in recycled manure solids bedding on Midwest dairy farms
Authors: Pena Mosca F. , Godden S. M. , Royster E. , Albrecht D. , Wells S. , Crooker B. , Aulik N.
Published: July 2023

While many dairies use green (GRN) recycled manure solids (RMS) bedding, some first process slurry through an anaerobic digester (DIG), while others have adopted secondary (SEC) pro­cessing methods such as mechanical composters (COM), hot air dryers (DRY), or, more recently, infrared drying (IR), in an effort to lower mastitis pathogen counts in ready to use (RTU) solids. However, these processing methods could also poten­tially reduce levels of other important pathogens in RTU solids. Our objective was to investigate the relationship between use of DIG and other SEC processing methods on M. avium subsp. paratuberculosis (MAP) and Salmonella spp. (SAL) in RTU RMS on Midwest farms.
Characterization of internal teat sealant behavior throughout the dry and post-fresh periods
Authors: Buckley M. P. , Silva G. S. , Tomazi T. , Miller B. E. , Bayne J. , Gorden P. J.
Published: July 2023

The primary objective of this study was to characterize the be­havior of internal teat sealants during the dry period as well as sealant shedding patterns during the post-fresh period in dairy cattle. Two commercially available teat sealants were utilized for this study ShutOutTM (Merck Animal Health, Madison, NJ) versus Orbeseal® (Zoetis, Parsippany, NJ). Digital radiography was used to monitor location and percentage of teat fill with sealant at dry off during the dry period. Other parameters eval­uated included weight of sealant shed at first milking, duration of sealant shedding after freshening, and amount of sealant shed at each milking. Incidence of clinical mastitis, teat-level infection status at dry off, and teat-level infection status during the dry and post-fresh periods were also assessed.
Assessing differences in early lactation milk constituent estimates between Holstein cows of varying health outcomes
Authors: McArt J. A. A. , Teplitz E. M. , Callero K. R. , Seminara J. A. , Frost I. R. , McCray H. A. , Martinez R. , Barbano D. M.
Published: July 2023

Cows have an increased risk of disease shortly after parturition due to the energy and macromineral demands that occur sec­ondary to the initiation of lactation. Our objective was to assess the feasibility of identifying a healthy or maladaptive transition using Fourier-transform infrared (FTIR) spectroscopy of milk.
Back Matter and Conference Newsletter
Authors: Wren Geni
Published: July 2023

55th AABP Conference Newsletter
Teranostyka raka stercza
Authors: Gronostaj Patrycja , Cholewiński Witold
Published: July 2023

Rolą teranostyki jest indywidualizacja postępowania diagnostycznego i leczniczego. Obecnie teranostyka znajduje najszersze zastosowanie w monitorowaniu i leczeniu guzów neuroendokrynnych i raka stercza. Istotą teranostyki jest dobór wskaźników preparatów radiofarmaceutycznych, umożliwiających obrazowanie i leczenie choroby. Wśród wyjątkowych w teranostyce radioizotopów, dodatkowo należy lutet-177 i aktyn-225.
Modification for Nitration of Halo-substituted meso-Tetraarylporphyrins: A Convenient
Scale-up in Small Amount of Solvent
Authors: Rosa Mariusz , Malinowski Maciej , Ostrowski Stanisław
Published: July 2023

The synthesis of porphyrins with halogens and nitro groups in meso-aryl rings is
described. Halo-substituted meso-tetraarylporphyrin derivatives in the reaction with sodium
nitrite in trifluoroacetic acid at 40°C (or room temperature) afford 5-(4-nitroaryl)-10,15,20-
triarylporphyrins. The above mono-nitro products bearing halogens on meso-aryl rings (F,
Cl, Br) are difficult to obtain selectively. The method elaborated herein allows to synthesize
them with reasonable yield (of up to 57%), and the reaction can be easily scaled-up. By this
route, the preparation of valuable substrates for further transformation to highly substituted
‘synthetic porphyrins’ was presented.
The Success of Serious Games and Gamified Systems in HIV Prevention and Care: Scoping Review
Authors: Jitmun Waritsara , Palee Patison , Choosri Noppon , Surapunt Tisinee
Published: July 2023

Background

AIDS, which is caused by HIV, has long been one of the most significant global public health issues. Since the beginning of the HIV epidemic, various types of nonelectronic communication tools have been commonly used in HIV/AIDS prevention and care, but studies that apply the potential of electronic games are still limited.

Objective

We aimed to identify, compare, and describe serious games and gamified systems currently used in HIV/AIDS prevention and care that were studied over a specific period of time.

Methods

A scoping review was conducted into serious games and gamified systems used in HIV prevention and care in various well-known digital libraries from January 2010 to July 2021.

Results

After identifying research papers and completing the article selection process, 49 of the 496 publications met the inclusion criteria and were examined. A total of 32 articles described 22 different serious games, while 17 articles described 13 gamified systems for HIV prevention and care.

Conclusions

Most of the studies described in the publications were conducted in the United States, while only a few studies were performed in sub-Saharan African countries, which have the highest global HIV/AIDS infection rates. Regarding the development platform, the vast majority of HIV/AIDS gaming systems were typically deployed on mobile devices. This study demonstrates the effectiveness of using serious games and gamified systems. Both can improve the efficacy of HIV/AIDS prevention strategies, particularly those that encourage behavior change.
Academic scientific schools of the Far East: sociological approach to the study of formation and development
Authors:   The Institute of history, archeology and ethnography people of the Far East, Dudarenok S. M.
Published: July 2023

In 2022, the Far-Eastern branch of the Russian Academy of Sciences celebrated its 90th anniversary. The Far-Eastern academic science is a notable education on the world scientific map. The world recognition of Far-Eastern academic science was brought by the works of the outstanding Far-Eastern scientists, creators and leaders of scientific schools, many of whom have no analogues in the world. The birth of scientific schools is associated with the names of outstanding scientists who have made a significant contribution to the methodology for the development of promising, most relevant areas of science. In addition, the science school is a good communication channel for the teacher's personal communication with their students, continuers of scientific traditions, research methodology and the main achievements of the founder of the school. At present, there is a need to prepare a reference book that will reflect the main milestones in the history and activities of scientific schools and scientific areas of the Far-Eastern branch of the Russian Academy of Sciences. When organizing the work on this topic, it is necessary to imagine not only what the phenomenon of scientific school is and how it should be studied, but also to identify how many scientific schools exist in the Far Eastern Branch of the Russian Academy of Sciences, who are their founders and leaders, to identify the most productive heads of scientific schools who have trained a significant number of students - candidates and doctors of sciences. This article is a kind of guideline for researchers in understanding the complex and multifaceted history of the Far-Eastern academic scientific schools.
Critical Rationalism as a Source of Theoretical Jurisprudence
Authors:   National Research University Higher School of Economics, Raab Ruslan S.
Published: July 2023

The article is devoted to the inquiry of a speciﬁc conception of law, emerging from an attempt to comprehend sociolegal order through the lens of epistemology of critical rationalism. Taking as an example the works of Friedrich Hayek, one of the leading theorists who applied critical-rationalist epistemology to the study of society, we describe the key tenets of this epistemology, as well as its implications for social science. According to our hypothesis, the key tenets of critical-rationalist epistemology can produce not only a certain social philosophy, but also a certain view on the main questions of legal theory. The questions of the function of law, its structure and evolution, being studied by its theoretical reconstruction in the context of the critical-rationalist epistemology, indicate the signiﬁcant relevance of the epistemology of critical rationalism as the basis of a coherent legal theory. The legal theory, which consider law as an endogenously evolving “compositive” (dispersed) social structure, preventing epistemically unjustiﬁed intervention of organized power actors into spontaneous social order. This perception of law, based on the epistemology of critical rationalism, opposes the currently dominant “Cartesian-rationalist” theory of law as an exogenously designed by political legislator logical normative structure, serving as a technical tool of epistemically unjustiﬁed intervention of organized power actors into spontaneous social order. And though this Cartesianrationalist legal theory is the opposite of the critical-rationalist concept of law, the very identiﬁcation of these two types of legal theory is becoming possible only when we analyze law through the lens of the epistemology of critical rationalism.
Abuse of Procedural Right in the Review of Judicial Acts in the Civil Process
Authors:   National Research University Higher School of Economics, Melnikova Anastasia V.
Published: July 2023

The purpose of this article is to analyze abuses of procedural right in civil and arbitrazh proceedings of the Russian Federation in the context of judicial review. The problem posed by the author is studied from “general” to “speciﬁc”: from the study of the potential for abuse of the right to appeal to the isolation and isolation of separate blocks of bad faith procedural behavior, typical for persons involved in the case. The author was able to identify four blocks of procedural abuse at the appeal stage: attempts to review judicial acts not in the manner prescribed by applicable law; circumvention of the prohibition to submit new evidence; inconsistent procedural conduct, as well as unreasonably prolonging the process of review. In addition, this article raises the question of the applicability of certain negative consequences for abusers in appellate and subsequent court proceedings. The article draws a conclusion about correlation of special norms (part 2 of article 111, part 5 of article 159 of the Commercial Procedure Code of the Russian Federation, article 99 of the Civil Procedure Code of the Russian Federation) with a basic general consequence of mala ﬁde procedural behaviour — refusal to satisfy claims of an abuser fully or partially.
The carbon footprint of different modes of birth in the UK and the Netherlands: an exploratory study using life cycle assessment
Authors: Heazell Alexander , Spil Nienke A. , Nieuwenhuizen Kim , Rowe Rachel , Thornton James , Murphy Elizabeth , Verheijen Evelyn , Shelton Clifford
Published: July 2023

Objective: To compare the carbon footprint of caesarean and
vaginal birth. Design: Life cycle assessment. Setting:
Tertiary maternity units and home births in the UK and the Netherlands
Methods: A life cycle assessment, including: equipment use,
energy, analgesia, hospital stay, waste, sterilisation and laundry, was
conducted using primary data combined with data from published sources.
Main Outcome Measures: ‘Carbon footprint’ (in kgCO
e) Results: Excluding analgesia, the carbon
footprint of a caesarean birth in the UK was 31.21 kgCO
e, compared with 12.47 kgCO e for
vaginal birth in hospital and 7.63 kgCO e at home. In
the Netherlands the carbon footprint of a caesarean was higher (32.96
kgCO e), but lower for vaginal birth in hospital and
home (10.74 and 6.27 kgCO e respectively). Emissions
associated with analgesia for vaginal birth were: 0.08 kgCO
e (opioid analgesia), 0.75 kgCO e
(remifentanil), 1.2 kgCO e (epidural) and 237.33 kgCO
e (nitrous oxide with oxygen). Differences in analgesia
use resulted in a lower average carbon footprint for vaginal birth in
the Netherlands than the UK (11.64 vs. 193.26 kgCO e).
Conclusion: The carbon footprint of a caesarean is higher than
for vaginal birth if analgesia is excluded, but this is very sensitive
to the analgesia used; use of nitrous oxide with oxygen multiplies the
carbon footprint of vaginal birth 25-fold. Alternative methods of pain
relief or nitrous oxide destruction systems would lead to a substantial
improvement in carbon footprint. Although clinical need and maternal
choice are paramount, protocols should consider the environmental impact
of different choices.
Type 2 Diabetes Polygenic Score Predicts the Risk of Glucocorticoid-Induced Hyperglycemia in Patients Without Diabetes
Authors: J. Deutsch Aaron , H. Schroeder Philip , Mandla Ravi , Kang Sarah , Erenler Feyza , M. Mercader Josep , S. Udler Miriam , C. Florez Jose , N. Brenner Laura
Published: July 2023

  

Objective: To assess whether increased genetic risk for type 2 diabetes (T2D) is associated with the development of hyperglycemia after glucocorticoid treatment. 

Research Design and Methods: We performed a retrospective analysis of individuals with no diagnosis of diabetes who received a glucocorticoid dose equivalent to 10 mg of prednisone or more. We analyzed the association between hyperglycemia and a T2D global extended polygenic score, which was constructed through a meta-analysis of two published genome-wide association studies. 

Results: Out of 546 individuals who received glucocorticoids, 210 developed hyperglycemia and 336 did not. The polygenic score was significantly associated with glucocorticoid-induced hyperglycemia (p = 0.038, OR = 1.4 per standard deviation of the polygenic score). 

 Conclusions: Individuals with increased genetic risk for T2D have a higher risk of glucocorticoid-induced hyperglycemia. This finding offers a mechanism for risk stratification as part of a precision approach to medical treatment.
Student Skills in Utilizing Information Technology in Guidance and Counseling Services as Preparation for the Innovative Technology Consciousness
Authors: Padmi Ni Made Diah , Marthen Hendry
Published: July 2023

Communication is one of the requirements in the implementation of the Guidance and Counseling service. During the Covid-19 Pandemic, the communication process could only be done through an online, including the Guidance and Counseling service at school. So that the application of Information and Communication Technology in Guidance and Counseling services becomes very important as a means of support in conveying information, namely service materials to support online learning activities. Current technological developments force BK teachers to continuously update their knowledge and skills in utilizing Information and Communication Technology. The purpose of this study is to describe the skills of students in utilizing Information Technology in Guidance and Counseling Services. The research method used is descriptive qualitative to describe the skills of students in utilizing various Information and Communication Technology facilities that can support Guidance and Counseling services in schools. Based on the research carried out, it was obtained an average score of 69 in the high, medium and low categories. Of the 70 students who participated, it showed that 51%, namely 36 students, had a score below the average ≤ 69.
Keragaman Bryophyta di Kawasan Wisata Gunung Pandan, Kabupaten Aceh Tamiang, Provinsi Aceh
Authors: Simarmata Putriani , Muhaini Ayuni , Ananda Julia , Lubis Parlindungan , Malau Marturia , Navia Zidni Ilman
Published: July 2023

Gunung Pandan Tourism is an area with waterfalls and rivers that has the potential to become a Bryophyte habitat. The aim of this study is to look into the diversity of Bryophyte in the Gunung Pandan tourist area. The exploratory method was used to collect data. The mosses discovered during the study were collected, herbarium specimens were made, and the mosses were identified. The results showed up of about 5 Bryophyte species, divided into 4 genera and 4 families. Bryophyte of all varieties can be found on rock, tree trunks, weathered wood, and soil substrates AbstrakWisata Gunung Pandan merupakan suatu kawasan yang berupa air terjun dan sungai sehingga berpotensi menjadi suatu habitat dari lumut. Penelitian ini bertujuan untuk mengkaji keanekaragaman lumut di kawasan wisata gunung pandan. Pengumpulan data dilakukan dengan metode eksplorasi. Lumut yang dijumpai selama penelitian dikoleksi, dibuat spesimen herbarium, dan diidentifikasi. Hasil penelitian diperoleh sebanyak 5 jenis lumut terdiri atas 4 marga dan 4 famili. Semua jenis Bryophyta ditemukan pada tipe substrat batu, batang pohon, kayu lapuk, dan tanah
Pengaruh Penambahan Variasi Jumlah Isolat Monascus purpureus Pada Sifat Organoleptik Teh Kombucha
Authors: Alhidayatullah Alhidayatullah , Hadijah Siti
Published: July 2023

Kombucha tea are fermentated drink of tea and sugar liquid used kombucha starter. The purpose of this study was to determine the effect of adding variations in the number of Monascus purpureus in organoleptic properties of kombucha tea.The research was used a faktorial complete random design with four treatments of isolate concentration i.e 1%, 2%, 3% and 0% (control). Organoleptic tests include taste, scent, and colour. The results showed that the addition of variations in the concentration of Monascus purpureus had an effect on scent and taste. Th highest average value based on the level of preference in organolptic test results includes the taste, scent, and colour of tea on isolate treatments respectively were 2% (3), 2% (3.6), 2% and control (3.6). The treatments with 2% isolates gave the best results on this study.AbstrakTeh kombucha adalah minuman hasil fermentasi larutan teh dan gula dengan menggunakan starter kombucha. Tujuan Penelitian ini dilakukan untuk mengetahui pengaruh penambahan variasi jumlah isolat Monascus purpureus pada sifat organoleptik teh kombucha. Penelitian dilakukan dengan menggunakan Rancangan Acak Lengkap dengan 4 perlakuan konsentrasi isolat yaitu: 1%, 2%, 3% dan 0% (kontrol). Uji organoleptik meliputi aroma, rasa, warna. Hasil penelitian menunjukkan bahwa penambahan variasi jumlah Monascus purpureus berpengaruh terhadap aroma dan rasa. Nilai rerata tertinggi berdasarkan tingkat kesukaan pada hasil uji organoleptik meliputi rasa, aroma, dan warna teh kombucha berturut-turut adalah pada perlakuan jumlah isolat 2% (3), 2% (3.6), 2% dan kontrol (3.6). Perlakuan jumlah isolat 2% memberikan hasil terbaik pada perlakuan yang diberikan