bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of SARS-CoV-2 Antigen Tests in Asymptomatic Testing of Passengers at German Airports under Time Constraints: Application of Three Different Antigen Test Formats
Authors: Pradas Laura , Viceconte Nikenza , Hannen Jennifer , Schölz Christian , Schubert Axel , Knote Robert , Wille Leon , Kleinow Peter , Heuer Andreas , Weckesser Volkmar , Hartkamp Jörg , Schaefer Stephan , Bauer Peter
Published: November 2021

People infected asymptomatically with SARS-CoV-2 can spread the virus very efficiently. To break infection chains, massive testing efforts are underway. While the value of RT-PCR in asymptomatic patients is established, point-of-care (POC) antigen tests against SARS-CoV-2 are considered inferior to RT-PCR in terms of sensitivity and specificity but have demonstrated utility, mostly in symptomatic patients. We compared the performance of three different antigen tests with colorimetric (Roche), fluorometric (Quidel Sofia 2), and instrument-based chemiluminescent (Fujirebio Lumipulse® G) readout. Sensitivities for Roche, Quidel, and Fujirebio were 62.5%, 90.9%, 97.5% (≤ct 26); 43.8%, 90.9%, 95.1% (≤ct 30); and 4.3%, 0.0%, 57.6% (˃ct 30), respectively. The two assays with increased sensitivity were employed to screen > 35,000 passengers at German airports under time constraints. Under real-world conditions, the rate of false positives was low: 0.15% (Quidel) and 0.06% for the instrument based Fujirebio assay. Our study exemplifies that antigen tests with enhanced detection methods have an acceptable sensitivity of >90% in samples containing SARS-CoV-2 RNA that are considered to be infectious. Therefore, our results support the view of the WHO that discourages the use of antigen assays with a sensitivity of “only” 80% for screening travelers.
Fabrication of La2O3/g-C3N4 Heterojunction with Enhanced Photocatalytic Performance of Tetracycline Hydrochloride
Authors: Zhu Zhengru , Xia Haiwen , Wu Rina , Cao Yongqiang , Li Hong
Published: November 2021

In this study, La2O3/g-C3N4 heterojunction photocatalysts doped with different dosages of La2O3 were constructed by a facile ultrasound-assisted calcination approach. The as-prepared photocatalysts were characterized by XRD, FTIR, FESEM, TEM, XPS, PL and DRS to verify the composite photocatalysts’ purity and to investigate their structural, morphological and elemental composition, and their energy band. According to the results, a type of pure rod–sheet-shaped, heterostructured nanoparticle was successfully obtained. Decorated with 10% La2O3, 2 g/L of the composite sample had a 93% degradation rate for 20 mg/L tetracycline hydrochloride within 2 h under visible light at a pH of 7. After four successive photocatalytic runs, satisfactory stability and reusability was exhibited, with 70% of the tetracycline hydrochloride being removed in the final experiment. Electrons (e−), photogenerated holes (h+), superoxide radical anions (·O2−) and hydroxyl radicals (·OH) were the fundamental active species during the photocatalytic process and were investigated via quenching experiments. Furthermore, possible photocatalytic mechanisms were analyzed in this work.
Evolutionary Ecology of Lizards: Lessons from a Special Issue
Authors: Zamora-Camacho Francisco Javier , Comas Mar
Published: November 2021

Regardless of taxonomical disquisitions on its yet unraveled phylogenetic relationships within and among taxa [...]
Assessment of the Elastographic and Electromyographic of Pelvic Floor Muscles in Postmenopausal Women with Stress Urinary Incontinence Symptoms
Authors: Ptaszkowski Kuba , Małkiewicz Bartosz , Zdrojowy Romuald , Paprocka-Borowicz Malgorzata , Ptaszkowska Lucyna
Published: November 2021

Background. Shear wave elastography is an effective method for studying the condition of various musculoskeletal soft tissues. The primary aim of this study was the objective elastographic and electromyographic assessment of the pelvic floor during the rest and contraction of the pelvic floor muscles (PFM) in postmenopausal women. Methods. This was a prospective observational study that was carried out at the University Hospital in Wroclaw, Poland, between January 2017 and December 2019. Patients. The target group of the study included postmenopausal women with stress urinary incontinence. The primary outcomes were the features of the elastographic assessment of the pelvic floor during rest and contraction of the PFM obtained using shear wave elastography. Results. Based on the inclusion and exclusion criteria for the study, 14 patients took part in the measurements. There was a significant difference between the elastographic assessment of the pelvic floor during rest and contraction of the PFM at all locations in front of the urethra. No statistically significant correlation was found between the results of elastography and the bioelectrical activity of PFM. Conclusion. The elasticity of the periurethral structures is higher during active pelvic floor muscle contraction than at rest, it seems that shear wave elastography is an effective test that objectively assesses the strength of PFM contraction.
Involvement of Ceramides in Non-Alcoholic Fatty Liver Disease (NAFLD) Atherosclerosis (ATS) Development: Mechanisms and Therapeutic Targets
Authors: Tanase Daniela Maria , Gosav Evelina Maria , Petrov Daniela , Jucan Alina Ecaterina , Lacatusu Cristina Mihaela , Floria Mariana , Tarniceriu Claudia Cristina , Costea Claudia Florida , Ciocoiu Manuela , Rezus Ciprian
Published: November 2021

Non-alcoholic fatty liver disease (NAFLD) and atherosclerosis (ATS) are worldwide known diseases with increased incidence and prevalence. These two are driven and are interconnected by multiple oxidative and metabolic functions such as lipotoxicity. A gamut of evidence suggests that sphingolipids (SL), such as ceramides, account for much of the tissue damage. Although in humans they are proving to be accurate biomarkers of adverse cardiovascular disease outcomes and NAFLD progression, in rodents, pharmacological inhibition or depletion of enzymes driving de novo ceramide synthesis prevents the development of metabolic driven diseases such as diabetes, ATS, and hepatic steatosis. In this narrative review, we discuss the pathways which generate the ceramide synthesis, the potential use of circulating ceramides as novel biomarkers in the development and progression of ATS and related diseases, and their potential use as therapeutic targets in NAFDL-ATS development which can further provide new clues in this field.
Do Sustainability Standards Exclude Small Farms? Modelling the Kenyan Floricultural Sector
Authors: Calisti Roberto , Holtmaat Ellen Alexandra
Published: November 2021

This study simultaneously addresses two issues: (a) defining what counts as ‘small farms’ in the rose sector, taking the geographical and socioeconomic context into account and (b) whether the requests for certification form barriers for small farms. We focus on small farms, as they are of fundamental importance for social and economic development and significantly contribute to the environmental sustainability of agriculture and land use. An agent-based model is used for analyzing an agricultural production and supply chain. The model identifies the minimum farm size needed to cover increased costs due to sustainability certifications. The model is applied to the case study of rose production in Kenya. Kenya is one of the world’s leading flower producers. Almost all Kenya’s floricultural production is exported, and the export of stem roses accounts for about 80% by weight of Kenya’s floricultural exports. Environmental and social sustainability certification is increasingly required for farms, especially those in developing countries that want to export their products. Our findings suggest that sustainability standards disadvantage small Kenyan rose farms and constitute a further obstacle to their entry into the international rose market. In this specific context, standards limit market access for farms smaller than 4 hectares. The agent-based model proposed in this study can be adjusted to help determine the definition of ‘small farms’ in need of extra support in other sectors.
Improving Text-to-Code Generation with Features of Code Graph on GPT-2
Authors: Paik Incheon , Wang Jun-Wei
Published: November 2021

Code generation, as a very hot application area of deep learning models for text, consists of two different fields: code-to-code and text-to-code. A recent approach, GraphCodeBERT uses code graph, which is called data flow, and showed good performance improvement. The base model architecture of it is bidirectional encoder representations from transformers (BERT), which uses the encoder part of a transformer. On the other hand, generative pre-trained transformer (GPT)—another multiple transformer architecture—uses the decoder part and shows great performance in the multilayer perceptron model. In this study, we investigate the improvement of code graphs with several variances on GPT-2 to refer to the abstract semantic tree used to collect the features of variables in the code. Here, we mainly focus on GPT-2 with additional features of code graphs that allow the model to learn the effect of the data stream. The experimental phase is divided into two parts: fine-tuning of the existing GPT-2 model, and pre-training from scratch using code data. When we pre-train a new model from scratch, the model produces an outperformed result compared with using the code graph with enough data.
Human Action Recognition of Spatiotemporal Parameters for Skeleton Sequences Using MTLN Feature Learning Framework
Authors: Mehmood Faisal , Chen Enqing , Akbar Muhammad Azeem , Alsanad Abeer Abdulaziz
Published: November 2021

Human action recognition (HAR) by skeleton data is considered a potential research aspect in computer vision. Three-dimensional HAR with skeleton data has been used commonly because of its effective and efficient results. Several models have been developed for learning spatiotemporal parameters from skeleton sequences. However, two critical problems exist: (1) previous skeleton sequences were created by connecting different joints with a static order; (2) earlier methods were not efficient enough to focus on valuable joints. Specifically, this study aimed to (1) demonstrate the ability of convolutional neural networks to learn spatiotemporal parameters of skeleton sequences from different frames of human action, and (2) to combine the process of all frames created by different human actions and fit in the spatial structure information necessary for action recognition, using multi-task learning networks (MTLNs). The results were significantly improved compared with existing models by executing the proposed model on an NTU RGB+D dataset, an SYSU dataset, and an SBU Kinetic Interaction dataset. We further implemented our model on noisy expected poses from subgroups of the Kinetics dataset and the UCF101 dataset. The experimental results also showed significant improvement using our proposed model.
A Design Procedure for Anchors of Floating Ocean Current Turbines on Weak Rock
Authors: Bañuelos-García Francisco , Ring Michael , Mendoza Edgar , Silva Rodolfo
Published: November 2021

In recent years, ocean current turbines have proven to be a reliable device for renewable energy generation. A crucial element of these turbines are the foundations, since they limit the displacement of the turbine, which is key in achieving efficiency in energy conversion, and can account for up to 26% of the total cost of the project. Most design procedures for foundations focus on sandy and clayey soils, but rock soils often predominate in tropical locations where marine currents are suitable for the installation of this type of turbine. This paper presents a design procedure for steel pile anchors (PAs) and concrete dead weight anchors (DWAs) on weak rock soils, using the assumptions of current technical documents and design codes commonly used in the industry for marine structures. Using specific designs for PA and DWA anchors, the procedure was theoretically assessed for a site off Cozumel Island, Mexico. The results show that the dimensions needed for DWAs are substantially larger than those for PAs. Therefore, whenever drilling is economically and operatively possible, piles would be preferable for the foundations of current turbine systems.
A Water Loop Design for the CRAFT Project towards the Testing of CFETR Water-Cooled Blanket and Divertor
Authors: Cheng Xiaoman , Liu Zihan , Liu Songlin , Peng Changhong , Wang Wenjia , Ling Qixin
Published: November 2021

As one of the tasks of the Comprehensive Research Facility for Fusion Technology (CRAFT), a High Heat Flux (HHF) testing device will be built to test the blanket and divertor of Chinese Fusion Engineering Testing Reactor (CFETR). The water loop is a key system of the HHF testing device. The main objective of the water loop is to provide deionized water at specific temperature, pressure, and flow rate for different testing experiments of the water-cooled blanket and water-cooled divertor components. The design of the water loop has been through three major steps. Firstly, the water cooled blanket and divertor were designed and analyzed, in detail, for CFETR. Secondly, thermal hydraulic features of the prototypes were abstracted from the analyses results. Then, the experiment plan was made so that the preliminary design of the water loop was carried out. The third step was the engineering design, which was conducted through cooperation with an industrial enterprise with certifications. At present, the water loop is ready for fabrication and construction. The water loop will be completed, for commissioning operation, by August 2022, as scheduled. After that, the experiments will be carried out step by step and provide solid technical base to CFETR.
Investment Model of Agricultural Biogas Plants for Individual Farms in Poland
Authors: Klimek Kamila , Kapłan Magdalena , Syrotyuk Serhiy , Bakach Nikolay , Kapustin Nikolay , Konieczny Ryszard , Dobrzyński Jakub , Borek Kinga , Anders Dorota , Dybek Barbara , Karwacka Agnieszka , Wałowski Grzegorz
Published: November 2021

The main idea of a circular economy (CE) is to separate economic growth from resource consumption and environmental impacts. The characteristic approach of a CE assumes the minimisation of the amount of waste generated at the design level and, as a standard, includes innovations throughout the value chain. From an agricultural point of view, agricultural biogas plants are particularly important because they enable the management of all waste biomass and its conversion into useful energy and agricultural fertiliser. This paper presents methods for assessing the economic effectiveness of an investment in an agricultural biogas plant. The research goal was to develop a financial model. The authors of this study used available examples of the profitability of commercial ventures. We considered the investment aspects of agricultural biogas plants. Exemplary solutions are discussed, allowing the reader to become acquainted with various methods and proposals for thus far estimated investments. It may seem chaotic, but this is how the biogas market is characterised in the context of the implementation of biogas projects. Guidance is given regarding how to understand investing in this sensitive private farming sector. It is admirable that the renewable energy market has been systematised, and we hypothesise that it is necessary to develop an investment model in Polish conditions.
Spatiotemporal Changes and Obstacle Factors of Forest Ecological Security in China: A Provincial-Level Analysis
Authors: Yu Honghong , Yang Jiameng , Qiu Mengyuan , Liu Zhiyong (John)
Published: November 2021

Under the background of China’s proposal to achieve “carbon neutralization and carbon peak”, it is an important task for each province to clarify their forest ecological security (FES) status. However, there is little understanding of the temporal and spatial evolution of forest ecological security and its influencing factors. Based on the Pressure-State-Response (PSR) model, this paper constructed a comprehensive evaluation index system for forest ecological security and used the CRITIC method and panel data to estimate the dynamic changes in FES for 31 provinces in China (excluding Hong Kong, Macao, and Taiwan) between 2009 and 2018. Furthermore, the obstacle degree model was used to determine the important obstacle factors affecting FES. The results showed that: (1) The comprehensive indices of FES of most provinces in mainland China were increasing, showing a good development trend during the study period; (2) subject to the limitations of resources and economic conditions, the FES at the provincial level showed significant spatial heterogeneity, which generally presents a distribution characteristic of “low in the western region and high in the central and eastern regions”; and (3) the primary obstacles restricting the improvement of FES level in most provinces of China were forest state indicators or input response indicators, followed by pressure indicators. Therefore, it is recommended to take targeted measures to reduce the intensity of forest cutting and the incidence of forest disasters, improve the utilization efficiency of forest resources, the productivity of forestland and the input-output level of forestry industry, and strengthen the training of professional talents and technical input according to the resource endowment condition of each province so as to improve the level of forest ecological security.
Succession of Microbial Communities of Corn Silage Inoculated with Heterofermentative Lactic Acid Bacteria from Ensiling to Aerobic Exposure
Authors: Guan Hao , Ran Qifan , Li Haiping , Zhang Xinquan
Published: November 2021

To further explore the effects of heterofermentative lactic acid bacteria (LAB) on silage fermentation and aerobic stability, whole-plant corn at around the 1/2 milk-line stage was freshly chopped and ensiled in laboratory silos with deionized water (control), Lactobacillus buchneri (LB), or L. rhamnosus (LR). Each treatment was prepared in triplicate for 3, 14, and 60 d of fermentation, followed by 3 and 7 days of aerobic exposure. The dynamic changes in microbial community were studied by single molecule real-time (SMRT) sequencing. The results showed that the two LAB inoculants altered the microbial communities in different ways. Succession from L. plantarum to L. buchneri and L. rhamnosus was observed in LB- and LR-treated silage, respectively. Both silages improved aerobic stability (82 and 78 h vs. 44 h) by occupying the microbial niche to produce higher levels of acetic acid at terminal fermentation. Because Acetobacter fabarum dominated in the silages after aerobic exposure, beta diversity dramatically decreased. In this study, a. fabarum was reported for the first time in silage and was related to aerobic spoilage. The two heterofermentative LAB produced acetic acid and improved the aerobic stability of the corn silage by occupying the microbial niche at terminal fermentation. Inoculated L. rhamnosus had a greater pH for a longer period of time after opening and less DM loss at day 7.
A CFD Tutorial in Julia: Introduction to Compressible Laminar Boundary-Layer Flows
Authors: Oz Furkan , Kara Kursat
Published: November 2021

A boundary-layer is a thin fluid layer near a solid surface, and viscous effects dominate it. The laminar boundary-layer calculations appear in many aerodynamics problems, including skin friction drag, flow separation, and aerodynamic heating. A student must understand the flow physics and the numerical implementation to conduct successful simulations in advanced undergraduate- and graduate-level fluid dynamics/aerodynamics courses. Numerical simulations require writing computer codes. Therefore, choosing a fast and user-friendly programming language is essential to reduce code development and simulation times. Julia is a new programming language that combines performance and productivity. The present study derived the compressible Blasius equations from Navier–Stokes equations and numerically solved the resulting equations using the Julia programming language. The fourth-order Runge–Kutta method is used for the numerical discretization, and Newton’s iteration method is employed to calculate the missing boundary condition. In addition, Burgers’, heat, and compressible Blasius equations are solved both in Julia and MATLAB. The runtime comparison showed that Julia with for loops is 2.5 to 120 times faster than MATLAB. We also released the Julia codes on our GitHub page to shorten the learning curve for interested readers.
An Approach to the Spanish Consumer’s Perception of the Sensory Quality of Environmentally Friendly Seabass
Authors: Calanche Morales Juan Benito , Tomás-Vidal Ana , Cusiyunca Phoco Edilson Ronny , Martínez-Llorens Silvia , Marquina Pedro L. , Jover-Cerdá Miguel , Roncalés Pedro , Beltrán José Antonio
Published: November 2021

Seabass is one of the leading aquaculture species in Europe. Sensory analysis is essential for new product development. This research focused on establishing and differentiating the opinion of consumers about seabass quality obtained with organic feeding. Fish were fed for 196 days with four treatments (a control diet with 30% fishmeal and three diets with different levels of fishmeal supplemented with organic vegetable ingredients: 25%, 30% and 35%). Experimental diets were compared with commercial samples from the retail industry that were considered as “adequate quality for fish”. Two sensory analyses were carried out, check-all-that-apply (CATA) to obtain feedback on consumers’ characterization towards a different type of fish evaluated and projective mapping (PM) to measure the similarity among a set of products and establish a comparison between results provided by both methods. According to the CATA results, white color, softness, meaty taste and juicy texture were considered relevant attributes, also showing a good relationship with an adequate cooked fish description. A penalty analysis confirmed that the previous characteristics were considered essential while fibrous was an undesirable attribute. The projective mapping showed a similar sensory configuration to the CATA, corroborating these findings that showed that commercial fish were placed in a position away from the rest of the treatments, and the organic diet with a higher level of fishmeal (35%) was the most distant from the control diet.
Comparing Taste Detection Thresholds across Individuals Following Vegan, Vegetarian, or Omnivore Diets
Authors: Jalil Mozhdehi Fatemeh , Abeywickrema Sashie , Bremer Phil James , Peng Mei
Published: November 2021

Taste perception plays an undisputed role in food choice, preference, and intake. Recent literature suggests that individual diet and taste sensitivity may have a reciprocal relationship, with evidence highlighting that specific diets can alter taste sensitivities. Commensurate with an increase in the prevalence of plant-based diets is the importance of investigating if following a vegetarian or vegan diet is associated with altered taste sensitivities. In this study, the taste detection thresholds for six compounds (i.e., sweet—sucrose, salty—sodium chloride, sour—citric acid, umami—monosodium glutamate, MSG, bitter—caffeine, and metallic—iron II sulphate heptahydrate) were measured for a total of 80 healthy, New Zealand European females aged 18–45 years old, who were categorised as 22 vegans, 23 vegetarians, and 35 omnivores. Each participant’s detection thresholds to these compounds were measured across two sessions, using an ascending Method of Limits with two-alternative-forced-choice presentations. The threshold data were analysed using both multivariate (i.e., principal component analysis) and univariate (i.e., ANCOVA) techniques to assess differences across the three types of diet. Multivariate analysis suggested that the omnivore group had distinct taste sensitivity patterns across the six compounds compared to the vegetarian or vegan group, which were characterised by relatively heightened sensitivity to metallic and lowered sensitivity to sweetness. Furthermore, the vegetarian group was shown to have a significantly lower detection threshold to bitterness (i.e., caffeine) relative to the other two groups (p < 0.001). While future study is required to investigate the cause–effect relationship between individual diet and taste sensitivities, the present study provides a systematic evaluation of taste sensitivities of individuals following distinct diets. This information may be valuable to future gustatory research as well as to food manufacturers.
Cocoa Coproducts-Based and Walnut Oil Gelled Emulsion as Animal Fat Replacer and Healthy Bioactive Source in Beef Burgers
Authors: Botella-Martinez Carmen , Lucas-González Raquel , Lorenzo José M. , Santos Eva María , Rosmini Marcelo , Sepúlveda Néstor , Teixeira Alfredo , Sayas-Barberá Estrella , Pérez-Alvarez Jose A. , Fernandez-Lopez Juana , Viuda-Martos Manuel
Published: November 2021

The aim of this work was to evaluate the effects on the chemical, physic-chemical, technological, and sensory properties of beef burger when replacing different quantities of fat (50 and 100%) with different levels of oil-in-water-gelled emulsion elaborated with walnut oil and cocoa bean shell flour (GECW). The chemical composition of the samples was affected by the fat replacement. The reformulation increased the moisture and ash content while the fat and protein content decreased with respect to the control sample. The linolenic and linolenic acid content of the beef burgers increased as the GECW replacement was augmented. The polyunsaturated fatty/saturated fatty acid ratio increased in both raw and cooked burgers, whereas the atherogenicity index and thrombogenicity index were reduced in both raw and cooked burgers with respect to the control sample. The use of GECW as a fat replacer was found to be effective in improving the cooking loss. Similarly, there were positive effects on reductions in the diameter and the increases in the thickness of the beef burgers. Regarding lipid stability, in both the raw and cooked burgers, the reformulation increased the 2-thiobarbituric acid reactive substance (TBARs) values with respect to the control sample. In both types of reformulated burgers, three bound polyphenols (mainly catechin and epicatechin) and two free polyphenols were identified, as were methylxanthines theobromine and caffeine. The sensory properties for the control and partial pork backfat replacement treatments were similar, while the sample with the total pork backfat replacement treatment showed the lowest scores. The blend of cocoa bean shell flour and walnut oil could be used as new ingredients for the development of beef burgers with a healthier nutritional profile without demeriting their sensory or cooking characteristics and physic-chemical properties.
Crystallization Behavior and Quality of Frozen Meat
Authors: Dang David S. , Bastarrachea Luis J. , Martini Silvana , Matarneh Sulaiman K.
Published: November 2021

Preservation of meat through freezing entails the use of low temperatures to extend a product’s shelf-life, mainly by reducing the rate of microbial spoilage and deterioration reactions. Characteristics of meat that are important to be preserve include tenderness, water holding capacity, color, and flavor. In general, freezing improves meat tenderness, but negatively impacts other quality attributes. The extent to which these attributes are affected depends on the ice crystalline size and distribution, which itself is governed by freezing rate and storage temperature and duration. Although novel technology has made it possible to mitigate the negative effects of freezing, the complex nature of muscle tissue makes it difficult to accurately and consistently predict outcome of meat quality following freezing. This review provides an overview of the current understanding of energy and heat transfer during freezing and its effect on meat quality. Furthermore, the review provides an overview of the current novel technologies utilized to improve the freezing process.
Apple Polyphenols Extract (APE) Alleviated Dextran Sulfate Sodium Induced Acute Ulcerative Colitis and Accompanying Neuroinflammation via Inhibition of Apoptosis and Pyroptosis
Authors: Liu Fang , Wang Xinjing , Cui Yuan , Yin Yan , Qiu Dong , Li Shilan , Li Xinli
Published: November 2021

The main aim of this study was to investigate the potent anti-apoptosis and anti-pyroptosis effects of apple polyphenols extract (APE) on dextran sulfate sodium model group (DSS)-induced acute ulcerative colitis (UC) and the protective effect of APE against acute UC-related neuroinflammation and synapse damage. Forty-three C57BL/6 male mice were randomly divided into a control group (CON), a 3% DSS model group (DSS), a 500 mg/(kg·bw·d) APE group (HAP), and a 125 (LD) or 500 (HD) mg/(kg·bw·d) APE treatment concomitantly with DSS treatment group. The results showed that APE significantly ameliorated DSS-induced acute UC through inhibiting intestinal epithelial cell (IEC) apoptosis and the Caspase-1/Caspase-11-dependent pyroptosis pathway, with increased BCL-2 protein expression and decreased protein levels of NLRP3, ASC, Caspase-1/11, and GSDND. Furthermore, APE significantly reduced acute UC-related neuroinflammation and synapse damage, supported by decreased mRNA levels of hypothalamus Cox-2 and hippocampus Gfap and also increased the mRNA levels of hypothalamus Psd-95. The increased protein expression of ZO-1 and Occludin improved the intestinal barrier integrity and improved the function of goblet cells by upregulating the protein level of MUC-2 and TTF3 accounted for the beneficial effects of APE on UC-associated neuroinflammation. Therefore, APE might be a safe and effective agent for the management of acute UC.
Neutron Stars and Gravitational Waves: The Key Role of Nuclear Equation of State
Authors: Koliogiannis Polychronis S. , Kanakis-Pegios Alkiviadis , Moustakidis Charalampos C.
Published: November 2021

Neutron stars are the densest known objects in the universe and an ideal laboratory for the strange physics of super-condensed matter. Theoretical studies in connection with recent observational data of isolated neutron stars, as well as binary neutron stars systems, offer an excellent opportunity to provide robust solutions on the dense nuclear problem. In the present work, we review recent studies concerning the applications of various theoretical nuclear models on a few recent observations of binary neutron stars or neutron-star–black-hole systems. In particular, using a simple and well-established model, we parametrize the stiffness of the equation of state with the help of the speed of sound. Moreover, in comparison to the recent observations of two events by LIGO/VIRGO collaboration, GW170817 and GW190425, we suggest possible robust constraints. We also concentrate our theoretical study on the resent observation of a compact object with mass ∼2.59−0.09+0.08M⊙ (GW190814 event), as a component of a system where the main companion was a black hole with mass ∼23M⊙. There is scientific debate concerning the identification of the low mass component, as it falls into the neutron-star–black-hole mass gap. This is an important issue since understanding the nature of GW190814 event will offer rich information concerning the upper limit of the speed of sound in dense matter and the possible phase transition into other degrees of freedom. We systematically study the tidal deformability of a possible high-mass candidate existing as an individual star or as a component in a binary neutron star system. Finally, we provide some applications of equations of state of hot, dense nuclear matter in hot neutron stars (nonrotating and rapidly rotating with the Kepler frequency neutron stars), protoneutron stars, and binary neutron star merger remnants.
Synthesis of Oxidant Functionalised Cationic Polymer Hydrogel for Enhanced Removal of Arsenic (III)
Authors: Song Yu , Gotoh Takehiko , Nakai Satoshi
Published: November 2021

A cationic polymer gel (N-[3-(dimethylamino)propyl]acrylamide, methyl chloride quaternary)(DMAPAA-Q gel)-supported oxidising agent (KMnO4 or K2Cr2O7) was proposed to remove As from water. The gel could adsorb arsenite, As(III), and arsenate, As(V), through the ion exchange method, where the oxidising agent oxidised As(III) to As(V). theoretically speaking, the amount of oxidant in the gels can reach 73.7 Mol%. The maximal adsorption capacity of the D-Mn gel (DMAPAA-Q gel carrying MnO4−) and D-Cr gel (DMAPAA-Q gel carrying Cr2O72−) for As(III) could reach 200 mg g−1 and 263 mg g−1, respectively; moreover, the As(III) removal rate of the gels could still be maintained above 85% in a neutral or weak acid aquatic solution. Studies on the kinetic and adsorption isotherms indicated that the As adsorption by the D-Mn and D-Cr gels was dominated by chemisorption. The thermodynamic parameters of adsorption confirmed that the adsorption was an endothermic process. The removal of As is influenced by the co-existing high-valence anions. Based on these results, the gels were found to be efficient for the As(III) adsorption and could be employed for the As(III) removal from the industrial wastewater.
Nephronophthisis-Pathobiology and Molecular Pathogenesis of a Rare Kidney Genetic Disease
Authors: Gupta Shabarni , Ozimek-Kulik Justyna E. , Phillips Jacqueline Kathleen
Published: November 2021

The exponential rise in our understanding of the aetiology and pathophysiology of genetic cystic kidney diseases can be attributed to the identification of cystogenic genes over the last three decades. The foundation of this was laid by positional cloning strategies which gradually shifted towards next-generation sequencing (NGS) based screenings. This shift has enabled the discovery of novel cystogenic genes at an accelerated pace unlike ever before and, most notably, the past decade has seen the largest increase in identification of the genes which cause nephronophthisis (NPHP). NPHP is a monogenic autosomal recessive cystic kidney disease caused by mutations in a diverse clade of over 26 identified genes and is the most common genetic cause of renal failure in children. NPHP gene types present with some common pathophysiological features alongside a diverse range of extra-renal phenotypes associated with specific syndromic presentations. This review provides a timely update on our knowledge of this disease, including epidemiology, pathophysiology, anatomical and molecular features. We delve into the diversity of the NPHP causing genes and discuss known molecular mechanisms and biochemical pathways that may have possible points of intersection with polycystic kidney disease (the most studied renal cystic pathology). We delineate the pathologies arising from extra-renal complications and co-morbidities and their impact on quality of life. Finally, we discuss the current diagnostic and therapeutic modalities available for disease management, outlining possible avenues of research to improve the prognosis for NPHP patients.
A Comprehensive Study of the Genus Sanguisorba (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis
Authors: Park Inkyu , Song Junho , Yang Sungyu , Choi Goya , Moon Byeongcheol
Published: November 2021

Sanguisorba, commonly known as burnet, is a genus in the family Rosaceae native to the temperate regions of the Northern hemisphere. Five of its thirty species are distributed in Korea: Sanguisorba officinalis, S. stipulata, S. hakusanensis, S. longifolia, and S. tenuifolia. S. officinalis has been designated as a medicinal remedy in the Chinese and Korean Herbal Pharmacopeias. Despite being a valuable medicinal resource, the morphological and genomic information, as well as the genetic characteristics of Sanguisorba, are still elusive. Therefore, we carried out the first comprehensive study on the floral micromorphology, palynology, and complete chloroplast (cp) genome of the Sanguisorba species. The outer sepal waxes and hypanthium characters showed diagnostic value, despite a similar floral micromorphology across different species. All the studied Sanguisorba pollen were small to medium, oblate to prolate-spheroidal, and their exine ornamentation was microechinate. The orbicules, which are possibly synapomorphic, were consistently absent in this genus. Additionally, the cp genomes of S. officinalis, S. stipulata, and S. hakusanensis have been completely sequenced. The comparative analysis of the reported Sanguisorba cp genomes revealed local divergence regions. The nucleotide diversity of trnH-psbA and rps2-rpoC2, referred to as hotspot regions, revealed the highest pi values in six Sanguisorba. The ndhG indicated positive selection pressures as a species-specific variation in S. filiformis. The S. stipulata and S. tenuifolia species had psbK genes at the selected pressures. We developed new DNA barcodes that distinguish the typical S. officinalis and S. officinalis var. longifolia, important herbal medicinal plants, from other similar Sanguisorba species with species-specific distinctive markers. The phylogenetic trees showed the positions of the reported Sanguisorba species; S. officinalis, S. tenuifolia, and S. stipulata showed the nearest genetic distance. The results of our comprehensive study on micromorphology, pollen chemistry, cp genome analysis, and the development of species identification markers can provide valuable information for future studies on S. officinalis, including those highlighting it as an important medicinal resource.
Fuzzy Cognitive Scenario Mapping for Causes of Cybersecurity in Telehealth Services
Authors: Poleto Thiago , Carvalho Victor Diogho Heuer de , Silva Ayara Letícia Bentes da , Clemente Thárcylla Rebecca Negreiros , Silva Maísa Mendonça , Gusmão Ana Paula Henriques de , Costa Ana Paula Cabral Seixas , Nepomuceno Thyago Celso Cavalcante
Published: November 2021

Hospital organizations have adopted telehealth systems to expand their services to a portion of the Brazilian population with limited access to healthcare, mainly due to the geographical distance between their communities and hospitals. The importance and usage of those services have recently increased due to the COVID-19 state-level mobility interventions. These services work with sensitive and confidential data that contain medical records, medication prescriptions, and results of diagnostic processes. Understanding how cybersecurity impacts the development of telehealth strategies is crucial for creating secure systems for daily operations. In the application reported in this article, the Fuzzy Cognitive Maps (FCMs) translated the complexity of cybersecurity in telehealth services into intelligible and objective results in an expert-based cognitive map. The tool also allowed the construction of scenarios simulating the possible implications caused by common factors that affect telehealth systems. FCMs provide a better understanding of cybersecurity strategies using expert knowledge and scenario analysis, enabling the maturation of cybersecurity in telehealth services.
Assessment of Pharmacists Prescribing Practices in Poland—A Descriptive Study
Authors: Zimmermann Agnieszka , Płaczek Jakub , Wrzosek Natalia , Owczarek Artur
Published: November 2021

Pharmacists play a beneficial role in supplying medicines to patients. Pharmacist prescribing practices were introduced into law in Poland in 2002, permitting pharmacists to prescribe medications in emergency situations and in 2020 the new law allowed to prescribe in all situation where it is needed because of the health risks reasons. Our aim was to analyze pharmacist prescribing practices in Poland and confirm the useful of pharmacists’ activity in this area. Additionally, pharmacists were also authorized to issue reimbursed prescriptions for themselves or their family members. Since January 2020, only e-prescriptions are allowed in Poland. A retrospective analysis of the inspection written reports from 842 community pharmacies in the representative region of Poland with a population of two million, carried out in the time period from 2002 to 2016 was performed (2189 prescriptions) to assess the emergency pharmacist prescribing practices in Poland. The second part of the research was based on digital data on pharmacists prescriptions (18,529) provided by the e-Health Centre (a governmental organization under the Ministry of Health responsible for the development of health care information systems in Poland), enabling to conduct the analysis of pharmacist’s prescribing from 1 of April 2020 to 31 of October 2020. The analysis gave the insight of the evolution of the pharmacy prescribing patterns. In general, pharmaceutical prescriptions were issued in cities with more than 100,000 inhabitants, in town- or city center pharmacies, and in pharmacies in residential areas. The most common reason for a pharmaceutical prescription was that the patient was running out of a medicine and was unable to contact their physician. Cardiovascular, respiratory, dermatological, and digestive medications were most frequently prescribed. An analysis of pharmacists’ prescribing data from 1 April 2020 to 31 October 2020 confirmed the rapid increase of pharmaceutical prescriptions following implementation of the new legislative act during the COVID-19 epidemic.
Change Management and Digital Innovations in Hospitals of Five European Countries
Authors: Hospodková Petra , Berežná Jana , Barták Miroslav , Rogalewicz Vladimír , Severová Lucie , Svoboda Roman
Published: November 2021

The objective of the paper is to evaluate the quality of systemic change management (CHM) and readiness for change in five Central European countries. The secondary goal is to identify trends and upcoming changes in the field of digital innovations in healthcare. The results show that all compared countries (regardless of their historical context) deal with similar CHM challenges with a rather similar degree of success. A questionnaire distributed to hospitals clearly showed that there is still considerable room for improvement in terms of the use of specific CHM tools. A review focused on digital innovations based on the PRISMA statement showed that there are five main directions, namely, data collection and integration, telemedicine, artificial intelligence, electronic medical records, and M-Health. In the hospital environment, there are considerable reservations in applying change management principles, as well as the absence of a systemic approach. The main factors that must be monitored for a successful and sustainable CHM include a clearly defined and widely communicated vision, early engagement of all stakeholders, precisely set rules, adaptation to the local context and culture, provision of a technical base, and a step-by-step implementation with strong feedback.
On What Do the Homeless Base Their Happiness?
Authors: Cano-González Yaiza , Portillo-Sotelo Carmen , Rodríguez-del-Águila María del Mar , García-Caro María Paz , Núñez-Negrillo Ana M. , Herrera-Espiñeira Carmen
Published: November 2021

Objective: To determine the relationship between the characteristics and experiences of homeless persons and their state of happiness as a basis for designing appropriate social support strategies. Design: Exploratory observational study with an analytical and descriptive qualitative design. Setting: Participants were contacted, administered with questionnaires, and interviewed in the street (central and northern areas of the city) or at the “Asociación Calor y Café” center in Granada (Spain) between April 2017 and February 2018. Participants: Selected by intentional sampling, 25 participants completed questionnaires in the first study and 14 of these were administered with questionnaires and interviewed in the second study. Method: General and specific questionnaires were administered to determine the state of happiness and other variables. Descriptive statistics were followed by an analysis of the relationships between variables and the content analysis of semi-structured interviews. Results: A feeling of happiness was described by 64% of participants and confirmed by a happiness scale score of 50%. Participants who felt satisfied with their life were 4.5-fold more likely to feel happy (p = 0.021). Expectations for the future were not associated with happiness or satisfaction with life. Content analysis of interviews revealed three main themes: conditions for happiness, own happiness/unhappiness, and self-esteem. Conclusions: Many homeless people describe themselves as feeling happy and satisfied with their life. Material aspects, affective situations, daily life concerns, and self-esteem predominate in their discourse on happiness.
Natural Plant Extracts and Microbial Antagonists to Control Fungal Pathogens and Improve the Productivity of Zucchini (Cucurbita pepo L.) In Vitro and in Greenhouse
Authors: Hassan Hanaa S. , Mohamed Abeer A. , Feleafel Mostafa N. , Salem Mohamed Z. M. , Ali Hayssam M. , Akrami Mohammad , Abd-Elkader Doaa Y.
Published: November 2021

Background: Natural plant extracts and microbial antagonists have the potential for use in increasing the fungal resistance and productivity of horticulture plants. Methods: The purpose of this study was to evaluate the ability of both natural plant extracts and microbial antagonists as a biotical control of some fungal pathogens, i.e., Fusarium ssp., Exserohilum ssp. and Nigrospora ssp., along with improving the growth and productivity performance of zucchini under greenhouse conditions. Eucalyptus camaldulensis leaf extract (LE), Citrus sinensis LE, Ficus benghalensis fruit extract (FE), and two microbial antagonists Pseudomonas fluorescens (accession no. MW647093) and Trichoderma viride (accession no. MW647090) were tested under in vitro and in vivo conditions. Through morphological characteristics and the internal transcribed spacer (ITS) region, Fusarium solani (accession no. MW947256), F. oxysporum (accession no. MW947254), Exserohilum rostratum (accession no. MW947255), and Nigrospora lacticolonia (accession no. MW947253) were identified. HPLC analysis was used for the identification of phenolic compounds (PCs) and flavonoid compounds (FCs) in the extracts. Results: The highest inhibition percentage of fungal growth (IPFG) against F. oxysporum was obtained with P. fluorescens, T. viride, and E. camaldulensis LE (4000 mg/L); F. solani with P. fluorescens, T. viride, and C. sinensis LE (4000 mg/L); Exserohilum rostratum with P. fluorescens, Ficus benghalensis FE (4000 mg/L) and E. camaldulensis LE (4000 mg/L), and N. lacticolonia with P. fluorescens. Using HPLC analysis, the abundant PCs in E. camaldulensis LE were pyrogallol, and caffeic acid, those in C. sinensis LE were syringic acid and ferulic acid, and those in F. benghalensis FE were gallic acid and syringic acid. In addition, the abundant FCs in E. camaldulensis LE were kaempferol, and naringin, those in C. sinensis LE were hesperidin and quercetin, and those in F. benghalensis FE were kaempferol and quercetin. Under greenhouse experiments, T. viride and E. camaldulensis LE (4000 mg/L) followed by P. fluorescens + T. viride treatments gave the best results of zucchini plants in terms of leaf area, fruits number per plant, yield per plant, and total yield (marketable and non-marketable). Conclusions: Plant extracts and bioagents can be used to control some zucchini fungal pathogens and increase the productivity performance of zucchini plants.