bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical efficacy of gamma-oryzanol combined with Femoston for perimenopausal syndrome
Authors: Kuang Yang-Yang , Xiong Min-Qi , Cai Jin-Xia
Published: June 2024

BACKGROUND

Perimenopausal syndrome (PMS) is a chronic disease associated with estrogen deficiency. Because of the unsatisfactory outcomes of current conventional treatments for this condition, its treatment must be continuously explored and optimized.

AIM

To assess the clinical effectiveness of γ-oryzanol in combination with Femoston for PMS.

METHODS

A total of 119 patients with PMS were selected from June 2023 to December 2023, which included 59 and 60 patients in the control and observation group, respectively. The control and observation groups were treated with Femoston and γ-oryzanol + Femoston, respectively. Comparative analyses were performed in terms of clinical effectiveness, safety (dizziness and headache, nausea and vomiting, and breast tenderness), sex hormones [estradiol (E2), luteinizing hormone (LH), and follicle-stimulating hormone (FSH)], lumbar spine (L1–4) and bilateral femoral bone mineral density (BMD), and sleep quality (sleeping time and frequency of awakenings from sleep).

RESULTS

Compared with the control group, the observation group had statistically higher total effective rates of treatment; lower overall incidence of adverse events; higher post-treatment E2 levels and L1–4 and bilateral femoral BMD; and lower LH and FSH levels, sleeping time, and frequency of awakenings from sleep after treatment.

CONCLUSION

Therefore, for the treatment of PMS, γ-oryzanol combined with Femoston is significantly better than Femoston alone in terms of clinical effectiveness, exhibiting more pronounced clinical advantages in improving safety, sex hormone levels, BMD, and sleep quality.
Mimicking aneurysm in a patient with chronic occlusion of the left middle cerebral artery: A case report
Authors: Yang Sen , Mai Rong-Kang
Published: June 2024

BACKGROUND

With the popularization of various cerebrovascular imaging methods and increased attention to the field, more cerebrovascular diseases are being detected in asymptomatic patients. Different cerebrovascular diseases are typically isolated but occasionally occur simultaneously, causing difficulties in diagnosis and treatment. Morphological changes in the collateral circulation of blood vessels in chronic cerebral artery occlusion patients are slow and dynamic, intercepting morphological development at a specific moment. Excessive reliance on single imaging tests such as digital subtraction cerebral angiography (DSA) can lead to misdiagnosis.

CASE SUMMARY

We report a 52-year-old male who was admitted to our department for treatment of an unruptured aneurysm during a follow-up examination for brain trauma after 1 mo. Computed tomography (CT) scan was negative, but CT angiography (CTA) revealed a sac-like bulge at the bifurcation of the left middle cerebral artery. DSA revealed an unruptured aneurysm with unique scapular morphology. The stump of a middle cerebral artery occlusion was observed during exposure during aneurysm clipping surgery, and the diagnosis of chronic cerebral artery occlusion was confirmed intraoperatively. This case was confusing because of the peculiar morphology of the arterial stump and compensatory angiogenesis due to multiple cerebral artery stenoses observed on preoperative CTA and DSA. The surgery did not cause secondary damage to the patient, and medical treatment for risk factors was continued postoperatively.

CONCLUSION

Multiple cerebral arterial stenoses can occur in conjunction with aneurysms or arteriovenous malformations, and their unique morphology can lead to misdiagnosis.
Lymphovenous anastomosis and complex decongestive therapy for severe deformed lymphedema with recurrent infection: A case report
Authors: Wang He-Jun , He Qing-Qing , Liu Chang-Rui , Wang Ying-Ying , Liu Xun-Wei
Published: June 2024

BACKGROUND

Lower extremity lymphedema is a common complication following treatment for gynecological malignancies. Its incidence rate can reach up to 70%, affecting ~20 million people worldwide. However, specialized treatment centers are scarce, and there is a lack of consensus on treatment approaches. Furthermore, there are even fewer reports on the systematic and effective treatment of severe lymphedema with malformations. Effective management of this condition remains a significant challenge for clinicians.

CASE SUMMARY

A 40-year-old woman developed bilateral leg swelling 6 years after receiving treatment for endometrial cancer. Since August 2018, she experienced > 30 episodes of lymphangitis. Upon presentation, she exhibited bilateral leg swelling and deformation, with four large swellings in the posterior thigh that impeded movement, and pain in the limbs. Skin manifestations included lichenoid lesions and features of deep sclerosis. Radionuclide lymphoscintigraphy confirmed the diagnosis of lower limb lymphedema. After 6 mo of complex decongestive therapy (CDT) and three lymphaticovenous anastomosis (LVA) treatments, the patient lost 49 kg in weight. She also experienced a maximum circumference reduction of 35.2 cm in the left lower limb and 37.5 cm in the right lower limb. The leg pain disappeared, her swelling significantly decreased, and she regained the ability to walk, cycle, and run normally.

CONCLUSION

The combined application of CDT and LVA therapy demonstrates significant positive effects in the treatment of severe, deformed stage III lymphedema.
Glucocorticoid therapy in pancreatic portal hypertension associated with autoimmune pancreatitis: A case report
Authors: Zhang Yi , Li Zhao-Dong , He Yuan-Jing , Peng Wei , Luo Yu-Jun , Li Xiao-An
Published: June 2024

BACKGROUND

Autoimmune pancreatitis (AIP) is a chronic form of pancreatitis characterized by diffused enlargement of the pancreas and irregular stenosis of the main pancreatic duct. Some studies have reported that AIP can cause hemorrhage of gastric varices (GV) related to portal hypertension (PH). However, such cases are rare. In addition, the association of PH with AIP is unclear. At the same time, the efficacy and duration of glucocorticoid therapy is also controversial.

CASE SUMMARY

In this case, we reported a case of GV in pancreatic PH associated with AIP. Enhanced abdominal computed tomography (CT) suggested splenic vein (SV) and superior mesenteric vein (SMV) thromboses. The patient received a long-term glucocorticoid therapy, that the initial dose of 40 mg is reduced weekly by 5 mg, and then reduced to 5 mg for long-term maintenance. CT and gastroscopic examination after 8 mo of treatment indicated that SV and SMV were recanalized, pancreatic stiffness and swelling were ameliorated, and the GV almost completely disappeared.

CONCLUSION

Long-term glucocorticoid therapy can alleviate the development of GV in patients with AIP and has potential reversibility.
Giant retroperitoneal hemolymphangioma: A case report and review of literature
Authors: Wang Yi-Kai , Liu Ying-Hao , Shuang Wei-Bing
Published: June 2024

BACKGROUND

Hemolymphangioma is a very rare benign tumor in clinical practice caused by abnormalities of the vasculature. Its clinical features are often atypical, and it is easy to miss and misdiagnose. When the time of nuclear magnetic T1 is significantly reduced, the diagnosis of hemangioma should be considered. Therefore, we report this case in the hope of raising clinicians' awareness of the disease.

CASE SUMMARY

A 37-year-old man presented with a giant retroperitoneal hemolymphangioma. Computed tomography and magnetic resonance imaging indicated the possibility of a large perirenal lymphatic cyst. The postoperative pathological diagnosis is retroperitoneal hemolymphangioma. The patient underwent surgical excision after adequate drainage. The postoperative recovery was smooth and there were no complications. There was no recurrence during half a year of follow-up.

CONCLUSION

This case reiterates that large retroperitoneal cystic masses with significantly shortened nuclear T1 time should be considered hemolymphangioma. Specific clinical basis and experience for the diagnosis and treatment of these diseases is necessary.
Selective his bundle pacing eliminates crochetage sign: A case report
Authors: Mu Yan-Guang , Liu Ke-Sen
Published: June 2024

BACKGROUND

Crochetage sign is a specific electrocardiographic manifestation of ostium secundum atrial septal defects (ASDs), which is associated with the severity of the left-to-right shunt. Herein, we reported a case of selective his bundle pacing (S-HBP) that eliminated crochetage sign in a patient with ostium secundum ASD.

CASE SUMMARY

A 77-year-old man was admitted with a 2-year history of chest tightness and shortness of breath. Transthoracic echocardiography revealed an ostium secundum ASD. Twelve-lead electrocardiogram revealed atrial fibrillation with a prolonged relative risk interval, incomplete right bundle branch block, and crochetage sign. The patient was diagnosed with an ostium secundum ASD, atrial fibrillation with a second-degree atrioventricular block, and heart failure. The patient was treated with selective his bundle pacemaker implantation. After the procedure, crochetage sign disappeared during his bundle pacing on the electrocardiogram.

CONCLUSION

S-HBP eliminated crochetage sign on electrocardiogram. Crochetage sign may be a manifestation of a conduction system disorder.
Short Communication: Investigation of mecA-positive and Methicillin-Resistant Staphylococcus aureus (MRSA) in dairy goat with subclinical mastitis from traditional farms in Banyuwangi, Indonesia
Authors: PRAJA RATIH NOVITA , EDILA RYANKA , YUDHANA ADITYA , SAPUTRO AMUNG LOGAM , HAMONANGAN JONATHAN MARK , PRAJA SHIFA SALSABILLA
Published: June 2024

Abstract. Praja RN, Edila R, Yudhana A, Saputro AL, Hamonangan JM, Praja SS. 2024. Short Communication: Investigation of mecA-positive and Methicillin-Resistant Staphylococcus aureus (MRSA) in dairy goat with subclinical mastitis from traditional farms in Banyuwangi, Indonesia. Biodiversitas 25: 1638-1643. Raw milk has the potential to transmit bacteria resistant to antibiotics and serve as a medium for the development of food-borne illnesses. Staphylococcus aureus is a harmful bacterium that can infect host cells and carry antibiotic-resistant genes. This study aims to investigate the occurrence of Methicillin-Resistant Staphylococcus aureus (MRSA) which contains mecA coding gene from raw goat milk in Banyuwangi, East Java, Indonesia. 150 raw milk samples were collected from five goat farms in Banyuwangi, East Java, Indonesia. S. aureus was isolated using selective media, and antibiotic susceptibility testing was conducted using the Mueller-Hinton agar diffusion method. MRSA was confirmed using the oxacillin and cefotaxime resistance screen agar test, with the presence of mecA genes identified through polymerase chain reaction. The results show that the prevalence of S. aureus was 30.6%. Of the 46 S. aureus isolates, 28 (60.87%) were categorized as MRSA. The mecA gene was observed in 9 (32.14%) MRSA isolates. The presence of MRSA in raw goat milk poses a significant public health threat, as bacteria carrying the mecA gene for antimicrobial resistance can spread through animal products like raw milk. Enhanced hygiene and sanitation protocols in dairy goat farms are essential prevention measures that must be strictly implemented.
Tropical gastropod density and diversity in the mangrove forest of Totobo Village, Southeast Sulawesi, Indonesia
Authors: PURNAMA MUHAMMAD FAJAR , PRAYITNO SLAMET B. , MUSKANANFOLA MAX R. , SURYANTI SURYANTI
Published: June 2024

Abstract. Purnama MF, Prayitno SB, Muskananfola MR, Suryanti. 2024. Tropical gastropod density and diversity in the mangrove forest of Totobo Village, Southeast Sulawesi, Indonesia. Biodiversitas 25: 1663-1675. The mangrove ecosystem of Totobo Village, Southeast Sulawesi, is a habitat for various species of edible and economically important gastropods. This research aims to determine mangrove gastropods' density and ecological index (diversity). This research was carried out from June to September 2023 in the mangrove ecosystem of Totobo. This research adopts purposive sampling and systematic random sampling techniques to determine stations and place the distribution of sampling points (sub-stations). The gastropod sample collection was done using the handpicking method or manually. More than 34 species of gastropods (14 families and 24 genera) were found in the mangrove ecosystem of Totobo. The diversity index (H' = 3.07) of gastropods in the Totobo mangrove ecosystem is directly proportional to the species richness value (R = 4.43), which is in the high category. The evenness index is in the medium category (E = 0.89), and the dominance index (C) has a value of 0.01, meaning no dominance of a particular species. The condition described is a representation of a system of homeostasis or environmental balance that is currently being maintained. This directly impacts the optimal biological activity (physiology) of the gastropod community and other aquatic organisms in the mangrove ecosystem (estuary) of Totobo Village.
A phenology-based linear spectral unmixing method for rice varieties identification from Landsat 8 Satellite Image in Ngawi District, East Java, Indonesia
Authors: CIPTA IQBAL MAULANA , JAELANI LALU MUHAMAD , SANJAYA HARTANTO
Published: June 2024

Abstract. Cipta IM, Jaelani LM, Sanjaya H. 2024. A phenology-based linear spectral unmixing method for rice varieties identification from Landsat 8 Satellite Image in Ngawi District, East Java, Indonesia. Biodiversitas 25: 1691-1702. Ngawi District is a prominent rice-producing district in East Java, Indonesia. Based on the BPS data from 2021, Ngawi District yielded a total of 786.49 thousand tons of paddy. Multiple strategies to enhance rice production include enhancing rice productivity, increasing paddy fields, and using effective land management techniques. Utilizing a superior breed of seeds can augment productivity. Remote sensing can be utilized to obtain such data, one of which is the Linear Spectral Unmixing (LSU) technique. The LSU approach is used to determine the proportion of a pure object's presence in a pixel. The Inpari 32 rice variety is the predominant type identified from the analysis of the processing outcomes utilizing satellite imagery. In addition, validation was performed by utilizing many sample locations distributed around Ploso Lor and Rejuno Villages, resulting in consistent findings between the data analysis and the actual conditions observed in the field. The RMSE calculation was conducted on both sets of processing results, and the most optimal RMSE value was achieved when processing was performed using Landsat 8 image data, yielding an RMSE value of roughly ±1.2%. The accuracy of the model was evaluated using a confusion matrix, which yielded an overall accuracy rate of 86.67%.
Segregation analysis and floral phenotype of T2 transgenic yellow cosmos (Cosmos sulphureus Cav.) carrying neomycin phosphotransferase II gene in second generation
Authors: MUCHYIDDIN MUHAMMAD GHAUTS AL AZAM , SAWITRI WIDHI DYAH , IRSYADI MUHAMMAD BURHANUDDIN , SWANDARI TANTRI , PURWANTORO AZIZ
Published: June 2024

Abstract. Muchyiddin MGAA, Sawitri WD, Irsyadi MB, Swandari T, Purwantoro A. 2024. Segregation analysis and floral phenotype of T2 transgenic yellow cosmos (Cosmos sulphureus Cav.) carrying neomycin phosphotransferase II gene in second generation. Biodiversitas 25: 1711-1717. The seed of transgenic yellow cosmos (Cosmos sulphureus Cav.) lines harbouring neomycin phosphotranferase II (nptII) gene have been obtained from our previous studies. The nptII selectable marker gene is commonly used for developing a method to estimate the number of targeted gene integrated into the genome. In order to produce successful clones, it is important to ensure that transgenes are inherited sexually as the dominant trait in T2 progeny. This study aimed to obtain the segregation patterns of nptII gene in two populations derived from open- and self-pollinated and evaluated floral phenotypic of T2 transgenic yellow cosmos. The molecular analysis of transgenic yellow cosmos was conducted by PCR and the obtained data was analyzed using a Chi-Square test. The results showed that transgenes were inherited in 1:1 for transgenic and wild-type in both self- and open-pollinated populations. Therefore, it indicated that T2 transgenic plants were still unstable and evolved continuously to segregate independently in the over generation. Surprisingly, the morphological changes were detected in transgenic plants. The mixture of ligulate and tubular types in ray floret was found in some of the flower types in T2 yellow cosmos. In this case, nptII gene may diverge in apparently random genome insertion. This insertion would induce morphological changes in the ray floret type of flower since recombination estimates varied among transformation events.
Carbon stock, density, and biomass of seagrass in South Bangka, Indonesia
Authors: SUPRATMAN OKTO , FARHABY ARTHUR MUHAMMAD , HENRI HENRI , SEPTIYATI MAHARRANI UTAMININGSIH , ADI WAHYU
Published: June 2024

Abstract. Supratman O, Farhaby AM, Henri, Septiyati MU, Adi W. 2024. Carbon stock, density, and biomass of seagrass in South Bangka, Indonesia. Biodiversitas 25: 1736-1742. As a component of the carbon ecosystem, seagrass meadows play a crucial role in preventing climatic change. The execution of Indonesian programs pertaining to carbon economic value, carbon trading, and other endeavors aimed at attaining the Nationally Determined Contribution (NDC) requires estimated data on seagrass carbon stocks. The present study investigates the seagrass density, vegetation carbon stock, and sediment carbon stock of South Bangka, Indonesia. The investigation is carried out in the following stages: i) data collection on-site, ii) laboratory sample analysis, and iii) data analysis. The accumulation of data on-site utilizes a square transect method, which includes the gathering of sediment, sample biomass, and density information. The carbon composition of sediment and seagrass vegetation is ascertained through laboratory analysis employing the Loss of Ignition (LOI) method. The investigation uncovered eight species of seagrass in South Bangka. Cymondocea rotundata, Enhalus acoroides, Halophila ovalis, Halodule pinifolia, Halodule uninervis, Oceana serrulata, Siringodium isotifolium, and Thalassia Hemprichii are the identified species of seagrass. In South Bangka, the combined carbon stock of seagrass (above and below ground carbon) varies between 0.049±0.057 and 1.323±1.581 Mg C Ha-1. The range of sediment carbon is 110.53±42.07 Mg Corg Ha-1 extrapolated to a depth of 1 m. The results of this study suggest that the seagrass meadows in South Bangka are characterized by a significant species diversity and effectively sequester carbon in the form of biomass and sediment. This supports the National Climate Change Mitigation Program for Blue Carbon Ecosystems, an initiative that seeks to mitigate the adverse effects of climate change by utilizing the carbon sequestration capabilities of mangrove forests, seagrass meadows, and tidal marshes.
Species composition and conservation of amphibians in Central Panay Mountain Range, Antique, Philippines
Authors: FLORES GERRIE MAE A. , TORRES ARMI G. , FLORES ANDRIE BON A. , JAKOSALEM PHILIP GODFREY C. , GUIHAWAN JAIME Q. , ROA-QUIAOIT HILLY ANN
Published: June 2024

Abstract. Flores GMA, Torres AG, Flores ABA, Jakosalem PGC, Guihawan JQ, Roa-Quiaoit HA. 2024. Species composition and conservation of amphibians in Central Panay Mountain Range, Antique, Philippines. Biodiversitas 25: 1797-1808. Assessing amphibian species composition and conservation status in Central Panay Mountain Range (CPMR), the largest contiguous forest in West Visayas in central Philippines, provides relevant and updated information on the status and conservation needs of the amphibians. Opportunistic, visual encounter and acoustic surveys were employed across diverse habitats-lowland and lower montane forests, streams, and grasslands. A total of 11 amphibian species from six families were documented, with notable findings including endemic species such as Rough-backed forest frog Platymantis corrugatus Duméril 1853, Dumeril’s wrinkled ground frog Platymantis dorsalis Duméril 1853, and Philippine narrow-mouth toad Kaloula conjuncta negrosensis Taylor 1922, as well as threatened species Panay forest frog Platymantis panayensis Brown, Brown and Alcala 1997, which is endemic to Panay Island. The P. dorsalis emerged as the most abundant, with 127 individuals recorded. Furthermore, two new distribution records of amphibians, narrow-mouth toad Kaloula sp. and panther flying frog Rhacophorus pardalis Günther 1858 were identified, potentially representing previously undocumented species endemic to the Philippines. The survey also revealed local threats posing significant risks to amphibian populations in the area. Several conservation measures have been proposed to protect amphibians and their habitats within CPMR. Given the area's rich biodiversity and ecological integrity, efforts are currently being implemented to establish CPMR as a nationally protected area, and this study contributes to this initiative by providing baseline data on amphibian diversity, underscoring the role of amphibians as ecological indicators of ecosystem health.
Selection assessment of promising forms of natural Hippophae rhamnoides (Elaeagnaceae) populations and their offspring in the Kazakhstan Altai Mountains
Authors: VDOVINA TATIANA A. , ISAKOVA ELENA A. , LAGUS OLGA A. , SUMBEMBAYEV AIDAR A.
Published: June 2024

Abstract. Vdovina TA, Isakova EA, Lagus OA, Sumbembayev AA. 2024. Selection assessment of promising forms of natural Hippophae rhamnoides (Elaeagnaceae) populations and their offspring in the Kazakhstan Altai Mountains. Biodiversitas 25: 1809-1822. This article presents the results of studying the genetic diversity of sea buckthorn, Hippophae rhamnoides L. in natural populations of the East Kazakhstan region. This made it possible to identify valuable forms based on which targeted breeding work was carried out. Selection work has shown that this species has a unique gene pool. As a result of the analytical selection of H. rhamnoides in the first, second, and third generations, promising seedlings were obtained: ´Podarok Bajtulinu Sh-9-81 (4-6) II´, ´Jubilejnaja Kotuhova T-2-82 (2-22) III´, ´Dolgozhdannaja ?5 (3-24) III´, ´Shetlastinka ?7 (2-24) III´, ´Nesravnennaja Sh-9-81 (3-27) III´, ´Krasavica Sh-9-81 (3-30) III´, ´Solnyshko Sh-12-81 (1-18) III´ with high yield, large fruits, long stalk and low fruit tearing force. Among the male plants, the following forms are distinguished: ´Gustoj Tuman T-2-82 (1-24) III´, ´Ljubimec T-17-82 (2-20) III´, ´Krasavchik K-8-82 (1-34) III´, ´Bogatyr' T-17-82 (2-20) III´ with high winter hardiness and pollen productivity. A scale has been developed for the force of fruit separation and the density of the “cob” for local forms. A selection achievement (variety) patent was received for the ´Jubilejnaja Kotuhova´ form. The next two forms (´Shetlastinka´ and ´Podarok Bajtulinu´) pass the state test for economic usefulness. The richest gene pool of sea buckthorn in the Altai mountainous country indicates its high heterogeneity.
The reproduction performance of catching Planiliza subviridis (Teleostei: Mugilidae) in different moon periods from Jepara Coast, Central Java, Indonesia
Authors: YUDIATI ERVIA , YUNIARTI TRISTIANA , TRIANTO AGUS , ARIFIN ZAENAL , RULIATY LISA , SOLEH M. , NARYANINGSIH AGUSTIEN , SURYATI SURYATI
Published: June 2024

Abstract. Yudiati E, Yuniarti T, Trianto A, Arifin Z, Ruliaty L, Soleh M, Naryaningsih A, Suryati. 2024. The reproduction performance of catching Planiliza subviridis (Teleostei: Mugilidae) in different moon periods from Jepara Coast, Central Java, Indonesia. Biodiversitas 25: 1823-1830. Mullets (Planiliza subviridis Valenciennes 1836) are highly promising species for aquaculture development. Information on the reproductive performance of wild-caught fish is crucial for enhancing mullet production. This study aimed to assess the gonadal maturity level of female mullets captured during full and new moon phases. Sampling using lift nets was conducted from June to July 2023, comprising 104 individuals, 51 during the new moon and 53 during a full moon. Fish caught during different moon phases were measured for gonad maturation level (GML) and morphometric parameters (body weight, total length, head length, body width, and head width), assessed for gonad somatic index (GSI), fecundity, and egg diameter. Over 50% of individuals were at GML III during dark moon phases, while during bright moon phases, only 10-15% were at GML III; GSI ranged from 0.6-15%. The fecundity of GML I and II females was higher during the full moon (220,212-395,000 eggs per individual) compared to the new moon, while GML III females during dark moon phases ranged from 225,000-250,000 eggs. Mullets exhibit increased reproductive output during the new moon phase and engage in synchronous spawning during moonlight cycles. Therefore, prospects for mullet culture appear promising, with cultivation and domestication suggested during new moon periods.
Plankton diversity in the Rowo Klampok Swamp, Malang District, East Java, Indonesia
Authors: ARFIATI DIANA , ZAKIYAH UMI , ANITASARI SEPTI , INAYAH ZAKIYYAH NUR , ORCHIDA KHARISMA , PRATIWI RIZKY KUSMA
Published: June 2024

Abstract. Arfiati D, Zakiyah U, Anitasari S, Inayah ZN, Orchida K, Pratiwi RK. 2024. Plankton diversity in the Rowo Klampok Swamp, Malang District, East Java, Indonesia. Biodiversitas 25: 1846-1855. Wetlands are a productive ecosystem and have high biodiversity, including plankton. Plankton can be used to assess the health status of wetlands. This research aims to determine the diversity and abundance of phytoplankton and zooplankton and to analyze the water quality conditions of the tropical wetland (Rowo Klampok Swamp, Malang District, East Java, Indonesia). This research was conducted from May to June 2023 and it's a survey research. The research results show that the phytoplankton found in Rowo Klampok Swamp consists of 4 divisions with 13 genera, while there are 3 divisions with 3 genera in zooplankton. Phytoplankton divisions are Bacillariophyta, Chlorophyta, Cyanophyta, and Dinoflagellate, while zooplankton divisions are Arthropoda, Ciliophora, and Rotifera. The average value of total phytoplankton abundance reached 2,618,684 cells/L, while zooplankton was 10,504 Ind/L. The Chlorophyta division is the most commonly found phytoplankton, with a percentage ranging between 32.5 and 53.1%, respectively. Dictyosphaerium is a phytoplankton genus often found with the highest average abundance of 1,922,929 cells/L. The diversity index, uniformity index, and dominance index of phytoplankton ranged between 1.965-2.439, 0.945-1.173, and 0.125-0.213, respectively, while those of zooplankton were 0.496-0.529, 0.317-0.593 and 0.458-0.684, respectively. Rowo Klampok Swamp has a medium trophic level (mesotrophic) and shows moderate pollution. The water quality conditions are considered suitable for aquatic organisms, but we need to monitor and reduce phosphorus input into the bodies of water.
Habitat of the amphibious mudskipper Periophtalmodon schlosseri in Songkhla Lake, Thailand
Authors: PATTARATUMRONG MANASAWAN SAENGSAKDA , POMPHA THANIDA
Published: June 2024

Abstract. Pattaratumrong MS, Pompha T. 2024. Habitat of the amphibious mudskipper Periophtalmodon schlosseri in Songkhla Lake, Thailand. Biodiversitas 25: 1875-1881. This study aimed to investigate the preferred habitat of giant mudskippers, Periophtalmodon schlosseri Pallas 1770. It was conducted from August to October 2022 at six sampling stations in Koh Yoh, a small island in the Lower Songkhla Lake. Environmental parameters such as organic matter and organic carbon in sediment, temperature, salinity, dissolved oxygen, and substrate were observed. The results indicate that the density of giant mudskipper weak to moderate correlates with all studied environmental parameters (P<0.05). Within the study area, the highest population density of P. schlosseri was recorded in the mudflat habitat adjacent to mangroves, and the lowest density was recorded in the mudflat habitat with a seawall, absent with nearby mangroves. This study suggests that environmental conditions affect the abundance of giant mudskippers, but this does not reach the limiting threshold; the more influential factor for this species is the character of the habitat. The mangrove habitat should be conserved and protected from development and urbanization. These results provide insights into the preferred habitat and offer a better understanding of the ecology of these fishes in the estuarine lake.
Short Communication: Potential threats to seagrass in the waters of Tanah Bumbu District, South Kalimantan, Indonesia
Authors: SALIM DAFIUDDIN , AMBO-RAPPE ROHANI , MASHORENG SUPRIADI , KADIR NADIARTI NURDIN
Published: June 2024

Abstract. Salim D, Ambo-Rappe R, Mashoreng S, Kadir NN. 2024. Short Communication: Potential threats to seagrass in the waters of Tanah Bumbu District, South Kalimantan, Indonesia. Biodiversitas 25: 1882-1889. Seagrass meadows are one of the most productive coastal communities, but they are easily degraded or lost due to declines in water quality. This study was conducted to analyze the potential threats to seagrass in the waters of Tanah Bumbu District, South Kalimantan, caused by increased turbidity from sedimentation and resuspension, the occurrence of invasive species, macroalgal and epiphytic cover on seagrasses. Field surveys on three coral cays (i.e. (Anugrah, Penyulingan and Katoang) and a literature study were conducted to collect data on sedimentation rate, total suspended solids (TSS), climate and precipitation, invasive species, and percentages of seagrass, macroalgae and epiphyte cover. Results showed a sedimentation rate of 46.66 mg/cm2/day and TSS range of 0.83-774.8 mg/l. These parameters were likely influenced by changes in land cover, sediment loads from surrounding rivers, climate, and port activity, especially at coal and palm oil terminals. An invasive species, i.e. zebra mussel (Dreissena spp.), was found on seagrass, and this is the first report of such an occurrence on seagrasses. The overall average percentage of seagrass, epiphyte and macroalgae cover on Anugrah, Penyulingan and Katoang coral cays were 33±26.78%; 37.68±29.31%; and 2.43±1.77%, respectively. The results of this study imply that seagrass ecosystems in Tanah Bumbu are threatened by increased turbidity, minimum light penetration, fluctuations in salinity, and the occurrences of competitors (invasive species and macroalgae).
Diversity of the genus Chondrilla L. (Asteraceae) in Eastern Europe
Authors: KASHIN ALEXANDR S. , PARKHOMENKO ALENA S. , KONDRATI ANNA O. , KRITSKAYA TATIANA A. , BOGOSLOV ARTEM V. , SHILOVA IRINA V.
Published: June 2024

Abstract. Kashin AS, Parkhomenko AS, Kondratieva AO, Kritskaya TA, Bogoslov AV, Shilova IV. 2024. Diversity of the genus Chondrilla L. (Asteraceae) in Eastern Europe. Biodiversitas 25: 1901-1910. The genus Chondrilla (Asteraceae) has a complex taxonomic structure due to widespread apomictic reproduction. This study aimed to identify the variability and clarify the status of Chondrilla species growing in Eastern Europe (European Russia and Western Kazakhstan). The study of morphological variability in Chondrilla species was assessed using 28 morphometric parameters. Moreover, 840 specimens from 28 populations of 8 Chondrilla species from Eastern Europe were analyzed. Principal Component Analysis (PCA), Nonmetric Multi-Dimensional Scaling (NMDS), recursive decomposition, and random forest algorithm were used for analysis. The ordination methods divided the studied samples into two groups: the first group included samples of C. ambigua and C. pauciflora, and the second group included all remaining samples. Following the resulting binary trees, obtained by recursive decomposition method and the random forest algorithm, a determination key was compiled for the Chondrilla species of Eastern Europe. The high level of morphological variation in Chondrilla and its distribution characteristics revealed in this study by numerical taxonomy and recursive decomposition methods, along with our previous studies results, show that only C. ambigua is a morphologically and genetically well-separated species, including C. pauciflora as a triploid cytotype; C. canescens, C. graminea, C. latifolia and C. juncea are synonyms with the priority name C. juncea and should be considered as subspecies; and C. brevirostris and C. laticoronata are hybrids with C. ambigua as maternal parent.
Profiling of microbiome biodiversity and structure associated with the gastrointestinal tract of Pacific white shrimp (Penaeus vannamei) using high-throughput sequencing
Authors: AMIN MUHAMAD , MUSDALIFAH LAILA , LEWARU MUHAMMAD WAHYUDIN , ALIMUDDIN ALIMUDDIN , ALIM SAHRUL , MARTIN MELISSA BEATA
Published: June 2024

Abstract. Amin M, Musdalifah L, Lewaru MW, Alimuddin, Alim S, Martin MB. 2024. Profiling of microbiome biodiversity and structure associated with the gastrointestinal tract of Pacific white shrimp (Penaeus vannamei) using high-throughput sequencing. Biodiversitas 25: 1984-1992. The Pacific white shrimp (Penaeus vannamei) holds significant importance as an aquaculture commodity in Indonesia, where the government actively promotes the expansion of brackish water pond intensification and extensification. Despite its global cultured prominence, there remains a research gap regarding the biodiversity of the microbiome within the gastrointestinal tracts (GITs) of cultured P. vannamei and the implications of aquaculture practices on microbiota dynamics. This study focuses on investigating the diversity and structure of the GIT microbiome in P. vannamei from selective intensive aquaculture ponds in East Java, Indonesia. Sampling from three distinct intensive rearing ponds, shrimp GITs were meticulously dissected to analyze the bacterial composition using high-throughput sequencing. The microbial diversity within the gastrointestinal tracts of these shrimps from commercial intensive aquaculture farms was evaluated through 16S rRNA amplicon sequencing, targeting the V3-V4 region, and the utilization of Operational Taxonomic Units (OTUs) for bacterial categorization. The results revealed that the gastrointestinal tract of shrimp reared in an intensive aquaculture system was dominated by nine bacterial phyla, namely Proteobacteria (53.95%), followed by Actinobacteria (26.78%), Bacteroidetes (3.95%), Firmicutes (2.41%), Tenericutes (1.98%), Chloroflexi (1.22%), Verrucomicrobia (1.03%), Cyanobacteria (0.71%) and Planctomycetes (0.65%). High levels of microbial diversity were indicated by the diversity indices, reflecting both richness (Simpson's index) and evenness (Shannon index). The findings contribute to a deeper understanding of microbiota dynamics in aquaculture systems and underline the significance of preserving microbial diversity for sustainable shrimp production.
Severe Verapamil Intoxication Requiring Plasmapheresis: A Case Report
Authors:   Department of Intensive Care Medicine, AZ Delta, Roeselare, Belgium., Dupon Valérie , Schockaert Bernard ,   Department of Intensive Care Medicine, AZ Delta, Roeselare, Belgium., Vandewaeter Catherine ,   Department of Intensive Care Medicine, AZ Delta, Roeselare, Belgium., Schoenmakere Gert De ,   Department of Nephrology, AZ Delta, Roeselare, Belgium.
Published: June 2024

Objective: Despite lack of evidence, plasmapheresis in severe verapamil intoxication may be indicated. Case Presentation: We present the case of a 44-year old man with life-threatening verapamil intoxication as a suicidal attempt. He was admitted to the emergency department with general malaise, dyspnea and abdominal pain. Despite supportive therapy, he became hypotensive and unresponsive. He was transferred to the intensive care unit for urgent plasmapheresis. Conclusion: Although scattered evidence suggests a possible benefit of plasmapheresis for severe verapamil intoxication, the EXTRIP workgroup recommends against. Keywords: Verapamil, Intoxication, Plasmapheresis, Calcium channel blocker
Infection After Total Knee Arthroplasty: Does Timing of Pre-operative Antibiotics Matter?
Authors:   [1] Northwell Orthopedics, New Hyde Park, New York, USA, [2] North Shore University Hospital, New York, USA, [3] Zucker School of Medicine, Hempstead, New York, USA, Walden Timothy J , Willinger Max L ,   [1] Northwell Orthopedics, New Hyde Park, New York, USA, [2] North Shore University Hospital, New York, USA, [3] Zucker School of Medicine, Hempstead, New York, USA, Heimroth Jamie C ,   [1] Northwell Orthopedics, New Hyde Park, New York, USA, [2] North Shore University Hospital, New York, USA, [3] Zucker School of Medicine, Hempstead, New York, USA, Strigenz Adam ,   North Shore University Hospital, New York, USA,, Sherman Alain ,   Northwell Orthopedics, New Hyde Park, New York, USA,, Danoff Jonathan R ,   [1] Northwell Orthopedics, New Hyde Park, New York, USA, [2] North Shore University Hospital, New York, USA, [3] Zucker School of Medicine, Hempstead, New York, USA
Published: June 2024

Background: Since the 2003 NIH consensus statement, the use of prophylactic antibiotics in total knee arthroplasty (TKA) has been the standard of care. Some studies recommend antibiotic administration within 1 h of skin incision, but no specific time frame has been delineated. The objective of this study was to determine if timing of pre-operative antibiotics is associated with post-operative infection after TKA. Materials and Methods: An institutional database from a multi-center health-care system was queried with ICD-10 codes and reviewed for patients undergoing primary TKA between March 2020 and December 2020. The rate of superficial surgical site infection (SSI) and periprosthetic joint infection (PJI) was compared with pre-operative antibiotic timing. PJIs were defined based on 2018 MSIS criteria and superficial SSIs were other infections that did not meet MSIS criteria. Antibiotic timing was separated into 15-min cohorts from 0 min before skin incision to over 45 min before incision. Further comparison between patients who received antibiotics within 30 min of incision with those who received antibiotics greater than 30 min prior was performed. Results: Of the 2511 patients who underwent primary TKA, 19 were found to have post-operative infections. There were 7 SSIs, and 12 PJIs, 16 of the post-operative infections occurred when patients received antibiotics <30 min before incision. There was no significant difference in SSIs or PJIs between each 15-min time interval of antibiotic administration (P = 0.45) or between the 30-min time intervals (P = 0.09). Conclusion: Our study demonstrates no difference in post-operative infection based on pre-operative antibiotic timing. As long as antibiotics are given within 60 min of incision, preferentially 30–60 min before incision, their timing does not have a significant effect on post-operative infection following TKA. Keywords: Pre-operative antibiotics, periprosthetic joint infection, superficial skin infection, total knee arthroplasty, complications
Comparative Study of BMD in Type 2 Diabetic and Non-diabetic Male Patients
Authors:   Department of Orthopedics, Max Superspeciality Hospital, Ghaziabad, Uttar Pradesh, India., Kumar Jata Shankar , Danish Mohd ,   Department of Orthopedics, Max Superspeciality Hospital, Ghaziabad, Uttar Pradesh, India., Singh Vikash ,   Department of Orthopedics, Max Superspeciality Hospital, Ghaziabad, Uttar Pradesh, India.
Published: June 2024

Introduction: Osteoporosis and diabetes are both common human diseases. The prevalence of both is increasing individually and in combination, due to better detection methods and changing definitions. Due to the different pathogenesis of Type 1 and Type 2 diabetes mellitus (T2DM), one of which is a predominant autoimmune process while the other mainly a metabolic disorder, it is not surprising that there is no uniform entity of diabetic bone disease as such, although such term has been proposed in the past but never gained momentum. Paradoxically, an increased risk of osteoporotic fracture in T2DM has been repeatedly demonstrated and this was independent of bone mineral density (BMD). This association with fracture adds uncertainty around the actual association between diabetes mellitus and BMD. This study aims to study the population of diabetes at tertiary care center when they are compared with non-diabetics in terms of BMD. Aims of Study: The aim of this study was to determine the prevalence of osteopenia and osteoporosis in T2DM and non-diabetic male patients using Dual Energy X-ray absorptiometry (DEXA scan). Materials and Methods: Patients for the study included male patients between 40 and 60 years of age group attending outpatient department, health checkup, and admitted in the ward of Saifee Hospital, Department of Medicine. In 200 (100 type 2 diabetic males and 100 non-diabetic males), DEXA Scan was performed in the Department of Imaging, Saifee Hospital from June 2017 to April 2019. Results: Type 2 diabetics were significantly associated with the presence of osteoporosis compared to non-diabetics (P = 0.001). Type 2 diabetics were significantly associated with body mass index (BMI) >25 (P = 0.0) and diabetics had a significantly higher BMI compared to non-diabetics (P = 0.0001). Type 2 diabetics above 50 years of age were significantly associated with osteoporosis (P = 0.000) and diabetics with osteoporosis were significantly older compared to diabetics without osteoporosis (P = 0.0018). Conclusion: The study concluded that there is a correlation between T2DM, increasing age, glycemic control, increased BMI, increased calcium levels, and decreased BMD. Thus, physician treating diabetes must anticipate decreased BMD and rule out or correct all of these factors in patients of diabetes to prevent the complications of decreased BMD in these groups of patients. Therefore, early detection and treatment of osteoporosis/osteopenia by estimation of BMD in Type 2 diabetic males, strict diabetic control with target hemoglobin A1c <6.5, weight control with target BMI <25, supplementation with Vitamin D3 should be advocated. Keywords: Diabetes, osteoporosis, dual-energy X-ray absorptiometry scan.
Potpourri – Recent and Relevant Literature in Periprosthetic Fractures Around the Knee
Authors:   [1] Centre for Bone and Joints, Kokilaben Dhirubhai Ambani Hospital and Medical Research Institute, Mumbai, Maharashtra, India, [2] Department of Orthopaedics, Holy Spirit Hospital, Andheri, Mumbai, Maharashtra, India., Desouza Clevio , Antao Nicholas ,   Department of Orthopaedics, Holy Spirit Hospital, Andheri, Mumbai, Maharashtra, India.
Published: June 2024

Knee replacement stands as an effective treatment for alleviating pain and restoring function in cases of degenerative joint conditions. The escalating prevalence of knee replacements reflects their acknowledged success. Despite being a relatively uncommon complication, periprosthetic fractures surrounding total knee arthroplasties present intricate challenges, demanding proficiency in both arthroplasty and trauma reconstructive techniques from treating surgeons. The confluence of increased life expectancy and elevated functional expectations among elderly individuals may contribute to a heightened occurrence of periprosthetic fractures. Among these fractures, supracondylar fractures of the femur emerge as the most prevalent. This comprehensive review delves into the classification and diverse treatment modalities available for addressing periprosthetic fractures around total knee arthroplasties. Keywords: Total Knee Arthroplasty, Replacement, Femur, Periprosthetic fractures
Bacterial Spectrum Analysis of Pediatric Septic Arthritis in Sanglah General Hospital (2019-2021): A Case Series
Authors:   Department of Orthopaedics and Traumatology, Faculty of Medicine Udayana University, Prof Ngoerah General Hospital, Denpasar, Indonesia, Caitanya Ida Bagus Anom Krishna , Maharjana Made Agus ,   Department of Orthopaedics and Traumatology, Faculty of Medicine Udayana University, Prof Ngoerah General Hospital, Denpasar, Indonesia, Ustriyana Nyoman Gede Grenata Nanda ,   Department of Orthopaedics and Traumatology, Faculty of Medicine Udayana University, Prof Ngoerah General Hospital, Denpasar, Indonesia
Published: June 2024

This case series presents five pediatric patients diagnosed with septic arthritis in Sanglah General Hospital from 2019 to 2021. The most common infecting organism was Staphylococcus aureus, comprising 66.7% of all infections. Other organisms described in literatures are Salmonella, Staphylococcus epidermidis, Enterobacter cloacae, and Mycobacterium tuberculosis. Antibiotic treatment should be started as soon as the diagnosis is suspected and modified once the organism isolated from the synovial fluid (SF) has been characterized. Unfortunately, in many cases, despite a high clinical suspicion of SA, the diagnosis cannot be confirmed because the SF is sterile on bacterial culture. This may lead to difficulties in patient management. The case series also analyzed the possible causes of negative culture results. The data showed three cases with no pathogen organism detected, which can be turned to false negative that ruled out the infection cause of the arthritis. The study suggests that in the future, other techniques might prove useful in increasing the sensitivity of the detection of bacterial infection, such as Polymerase chain reaction and detection of antibodies against the teichoic acid staphylococcal cell wall component. The detection and identification of Kingela kingae in synovial fluid have improved significantly since the development of PCR. The specificity of these assays relies on the ability of the PCR to generate only the expected amplification product. The duration of the laboratory to process is also a factor that affects the result. The study concludes that early diagnosis and prompt treatment are crucial in achieving good functional outcomes in pediatric septic arthritis. Keywords: septic arthritis, pediatric patients, bacterial spectrum, diagnosis, culture methods
The role of emotions in ontological conflicts: a case of study of the territorial–ontological conflict between British Columbia, Coastal GasLink and the Wet’suwet’en
Authors: Gálvez-Campos B.A.
Published: July 2024

Drawing on a methodological approach that involved visual ethnography and combined content and narrative analysis, my research aims to analyse the role that emotions play in the territorial–ontological conflict between British Columbia provincial government, Coastal GasLink and the Wet’suwet’en. Using high-quality online audiovisual material produced by the Wet’suwet’en – allowing a critical perspective throughout the article on the politics of self-representation – I was able to get into the conflict with a phenomenological approach, employing my senses to analyse body movements, tone of voice and language. Theoretically, I articulate a framework made up of Ingold’s phenomenology, Blaser’s ontological conflicts and Escobar’s studies of culture. Then, I build on the spiderweb, a metaphor developed by Ingold, to expand the scope of González-Hidalgo’s emotional political ecologies. The results show that Coastal GasLink, taking culture ‘as a symbolic structure’, proposes as a central mitigation strategy, through their environmental impact assessment, what I call ‘an ontological interruption’ of the Yintakh. Besides, I demonstrate that the processes of political inter-subjectivation sought at the Unist’ot’en Healing Centre help understand the worry, frustration and stress of the Wet’suwet’en facing the world-creating practices of Coastal GasLink. On the other hand, the Healing Centre also reveals how the affections for the other-than-human and their spiderweb (Yintakh or relational world) inform Wet’suwet’en resistance. Lastly, I unveil how Coastal GasLink and the Ministry of Aboriginal Rights, through practices of inclusion and gender equality, seek to blur radical cultural differences, delegitimise the Wet’suwet’en precolonial governance system, and create affections for the Western-modern world.