bluuebunny/crossref_metadata_2025 huggingface dataset:
γ-Aminobutyric Acid (GABA) Induced in Vitro Differentiation of Rat Pancreatic Ductal Stem Cells into Insulin-Secreting Islet-Like Cell Clusters
Authors: Ghani M. W. , Yi Z. , Jiang W. , Ye L. , Bin L. , Cun L. G. , Birmani M. W. , Mei Xiao
Published: October 2023

In vitro produced β-like cells can provide promising cell therapy for curing the epidemic of diabetes. In this context, we aimed to investigate the effects of different concentrations of γ-aminobutyric acid (GABA) on the differentiation of rat pancreatic ductal epithelial-like stem cells (PDESCs) into β-like cells. The PDESC line cells were cultured in the basal media (DMEM/F12 + 10% FBS + 1% penicillinstreptomycin) supplemented with 0 μM, 5 μM, 50 μM, 500 μM, and 5 mM of GABA for 28 days to induce their differentiation. The differentiated cells were detected by cell morphology, dithizone (DTZ) staining, immunofluorescence staining, real-time polymerase chain reaction (qPCR), and glucose-stimulated insulin secretion (GSIS) assay to validate their identity. At the end of 28 days, compared with the control group, enrichment of induced cells was high among the 5 μM, 50 μM, 500 μM, and 5 mM GABA induction groups. The formation of islet-like cell clusters (ICCs) began at 14 days, and the cell clusters showed a growth trend with the culture time. The induced ICCs were positive for DTZ staining, while the control group showed negative results for DTZ staining and the differentiated cells were also positive for β-cell-specific markers (Ins1 and Pdx1). GSIS assay of 50 μM induction group cells at 28 days showed significantly higher levels of C-peptide and insulin secretion than the control, 5 μM, 500 μM, and 5 mM GABA-treated groups (P < 0.01). At the same time, the 50 μM induction group cells also showed significantly higher levels of Ins1, Pdx1 and Nkx6.1 mRNA as compared to the 5 μM, 500 μM and 5 mM GABA groups (P < 0.01). Thus, the addition of GABA to the basal medium effectively induced differentiation of adult rat PDESCs into insulin-secreting β-like cells, and 50 μM was the most effective concentration for the induction.
New Developments in Understanding the Histological Structure of Human Ear Cartilage
Authors: Kaňa M. , Kaňa R. , Povýšil Ctibor
Published: October 2023

Histological, immunohistochemical and molecular examination of bioptic samples of 30 normal adult auricular cartilages and small samples from 6 ear cartilages from aborted foetuses was performed. The adult cartilage was the tissue with minimal proliferative activity, which we were able to confirm with antibodies against Ki67 in contrast to a high proliferative activity in the auricular cartilage of foetal tissues. It may therefore be presumed that the process of foetal tissue maturation is undoubtedly associated with a significant reduction in proliferative activity. The mature lamella of the adult auricular cartilage has a histological tri-lamellar structure. There are a great number of elastic fibres in the intercellular matrix of the central zone, which are conversely present in only small amounts in both peripheral layers. While the external layer of the concave surface of the cartilage contains a fewer number of oval elements, the external layer of the convex side is composed of numerous fusiform chondrocytes. Antibodies against various subtypes of S-100 protein showed that auricular chondrocyte activity is modified depending on the configuration of individual distinct zones (isoforms A1, A6, B2 and P were positive in all layers, isoforms A2 and A2 in peripheral zones). The most active cells metabolically are most likely chondrocytes in both external layers adjacent to the perichondrium. We have also demonstrated α-smooth muscle actin (SMA)-positive chondrocytes in both peripheral layers of the auricular cartilage adjacent to the perichondrium. In addition, we found definite differences in the distribution of actin-positive cells depending on the external shape of the pinna. The majority of these fusiform cells were localised primarily in the areas of great curvature of the pinna, especially the convex side, as mentioned above. On the basis of these unique structural features we assume that the ear cartilage may embody an example of the socalled intelligent biological material, which has its internal structure made in such a way as to more easily develop and yet still maintain all the shape characteristics of the human auricle. The knowledge of these specific structural characteristics is important especially for use of auricular cartilage in auricular reconstruction.
Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
Authors: Al Syaad K. M. , Elsaid Fahmy G. , Abdraboh M. E. , Al-Doaiss A. A.
Published: October 2023

Annona and ginger have prominent uses in traditional medicine; their therapeutic properties have not been sufficiently explored. The ameliorative effect of Annona or ginger extracts on hyperglycaemia associated with oxidative stress, inflammation, and apoptosis in experimentally induced diabetes was addressed. Type 1 diabetes in male rats was induced by a single injection of streptozotocin (STZ; 40 mg/kg, i.p.), then Annona (100 mg/kg) or ginger (200 mg/kg) extracts were orally administered daily for 30 days. The Annona and ginger extracts ameliorated hyperglycaemia, insulin level, glycosylated haemoglobin (HbA1c) and insulin resistance (HOMA-IR) levels in the diabetic rats. The treatments significantly ameliorated liver function enzymes and total proteins; this was confirmed by histopathological examination of liver sections. Annona and ginger extracts significantly reduced elevated malondialdehyde (MDA) and restored activity of antioxidant enzymes in the liver such as glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) and the hepatic content of reduced glutathione (GSH). The oxidative stressdependent inflammation was regulated by both Annona and ginger extracts, which was indicated by down-regulation of TNF-α, NF-κB, pro-apoptotic proteins Bax, p53, and anti-apoptotic protein Bcl-2. Moreover, the expression of insulin receptor (INSR) and glucose transporter 2 (GLUT2) genes was markedly regulated by both these extracts. The results suggest that Annona and ginger extracts ameliorate the hepatic damage resulting from diabetes by advocating antioxidants and modulating apoptotic mediator proteins in the liver of diabetic rats. In conclusion, Annona and ginger extracts have a potential therapeutic effect in the treatment of diabetes and its complications.
Engineering Drought Tolerance in Crops Using CRISPR Cas systems
Authors: Lola K. Kamalova , Mukhammadjon Mirzakhmedov , Mirzakamol Ayubov , Abdurakhmon Yusupov , Bekhzod Mamajonov , Nurdinjon Obidov , Ziyodullo Bashirkhonov , Anvarjon Murodov , Zabardast Buriev , Ibrokhim Abdurakhmonov
Published: October 2023

Drought stress is one of the most considerable threats to global agricultural food security, causing yield losses worldwide. Therefore, the search for effective genetic and molecular methods for developing cultivars that are tolerant or resistant to harsh environments has been more intensive over the last decades. Apart from time-consuming conventional breeding techniques, biotechnologists are now investigating modern genome editing tools for engineering tolerance and resistance to various biotic and abiotic stresses in crops. Various genetic engineering techniques such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) were developed based on the discovery of the DNA structure. However, these methods have limitations, with ZFNs being prone to errors due to their limited base pair recognition, and TALENs requiring a complex protein engineering process and struggling to cleave methylated DNA. In recent years, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) and its alternatives have gained popularity in plant biotechnology. Out of the genome editing techniques mentioned earlier, CRISPR/Cas9 is becoming more popular because it's faster and easier to use. Given that drought is now a significant threat to global agriculture due to the drying of arable lands, this review focuses on how we can use CRISPR genome editing to enhance crop tolerance to drought stress and explores its future potential.
A szoptató és a nem szoptató gyermekágyas anyák táplálkozási szokásainak összehasonlítása, valamint az anyatejes táplálást befolyásoló szociodemográfiai tényezők
Authors: Hulman Anita , Varga Katalin , Ádám Zsombor , Pakai Annamária
Published: October 2023

Bevezetés: Az anyatejes táplálás a csecsemő táplálásának
legideálisabb formája, a biológiai norma, amelyet az anya táplálkozása, valamint
bizonyos szociodemográfiai faktorok egyaránt befolyásolhatnak.
Célkitűzés: A jelen kutatás célja, hogy összehasonlítsa a
gyermekágyas időszakban a szoptató és a nem szoptató anyák táplálkozási
szokásait, továbbá megvizsgálja a szociodemográfiai tényezők összefüggéseit az
anyatejes táplálással. Módszer: Kvantitatív, keresztmetszeti
vizsgálatunkat 2021. 03. 26. és 2021. 07. 18. között végeztük. A nem
véletlenszerű, célirányos, szakértői mintaválasztás során a célcsoportba azok a
biológiai anyák kerültek, akik legalább egy, a 37. betöltött gestatiós hét után
élve született gyermeket neveltek saját gondozásukban. A kizárási kritériumok
közé tartozott az örökbefogadás, az első gyermekkel való várandósság, a kötelező
kérdések nem megfelelő kitöltése, valamint az anyának olyan, veleszületett
és/vagy szerzett testi, illetőleg mentális betegségéről való beszámoló, amely
miatt a szoptatás nem valósítható meg. A beválasztási és a kizárási
kritériumoknak 2008 fő felelt meg. A felmérés online formában, önkitöltős
kérdőíven keresztül, anonim módon történt. A statisztikai elemzést IBM SPSS 25
program segítségével végeztük, a szignifikanciaszintet p<0,05 értéknél
határoztuk meg. Eredmények: Szignifikáns különbség volt a
szoptató anyák és a nem szoptató anyák csoportja között az életkor (p<0,001),
a lakóhely (p<0,001), a családi állapot (p<0,001), az iskolai végzettség
(p<0,001), a jövedelmi státusz (p<0,001), a gyermekek száma (p = 0,005), a
szülés módja (p = 0,018) és aközött, hogy hányadik gyermekre vonatkoztatva
töltötték ki a kérdőívet (p = 0,021). A változatos táplálkozás jelentősen
nagyobb arányban volt jellemző a gyermekágyas időszakban a szoptató anyák
körében (p = 0,006). Kapcsolat mutatkozott az energiabevitelben (p = 0,002),
továbbá a napi vitamin-, ásványianyag-, nyomelembevitel (p = 0,044) tekintetében
is a két csoport között. Következtetés: A gyermekágyás időszak
alatt a szoptató anyák jobban odafigyelnek táplálkozásukra, mint a nem szoptató
anyák. Az anyatejes táplálást jelentős mértékben befolyásolhatják bizonyos
szociodemográfiai faktorok. Orv Hetil. 2023; 164(43): 1693–1700.
Anatomical and functional results after surgical treatment of uterine leiomyoma combined with genital prolapse
Authors:   Shupyk National Healthcare University of Ukraine, Kyiv, Baryshnikova O.P. , Chaika K.V. ,   Shupyk National Healthcare University of Ukraine, Kyiv, Mazur G.M. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Bevz G.V. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Sergiychuk O.V. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Vozniuk A.V. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Published: October 2023

More than 41% of women aged 50-79 suffer from pelvic organ prolapse. Genital prolapse is combined with uterine leiomyoma in approximately 20% of cases, which necessitates a differentiated approach to the treatment. Purpose - to compare the anatomical and functional results of surgical treatment of women with uterine leiomyoma combined with genital prolapse, who underwent hysterectomy with and without prolapse correction. Materials and methods. 80 patients with genital prolapse combined with uterine leiomyoma were examined and divided into three groups. In the Control (I) Group (n=40), hysterectomy was performed without correction of genital prolapse, in the other two groups - hysterectomy and correction of genital prolapse using a mesh implant by the method of pectopexy (the Group II, n=40) or lateral fixation (the Group III, n=40). Anatomical and functional outcomes were assessed 6 and 12 months postoperatively using the POP-Q and the questionnair PFDI-20 and PISQ-12. Statistical analysis was performed with SPSS Version 21.0. Results. In women of the Groups II and III, POP-Q parameters significantly improved 6-12 months after surgery compared to the initial level, while in patients of the Group I, 1 year after surgery, negative dynamics of changes in points Aa, Ba and indicators of pb and Tvl were established. Anatomical success, which is POP-Q stage 0-1 one year after surgery and does not require further surgical treatment, was 82.5% in the Group II and 85% in the Group III. The frequency of improvement of pelvic functions according to PEDI-20 and the state of sexual function according to PISQ-12 6 months after the operation was significantly higher among women of the Groups II and III comparing to the Group I. Conclusions. The obtained data testify to the anatomical effectiveness of simultaneous hysterectomy and correction of genital prolapse using mesh implants. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
The reproductive function of women with infertility after suffering COVID-19 infection
Authors:   Ivano-Frankivsk National Medical University, Ukraine, Golovchak I.S.
Published: October 2023

The aim was to assess the status of reproductive function of infertile women after COVID-19 infection. Materials and methods. The thorough examination included 120 patients who appealed to the reproductive clinic for infertility treatment. These women were divided into 2 groups: the main group - 80 women after COVID-19 infection (exclusion criteria - patients solely with the male factor of infertility), and the comparison group included 40 patients without COVID-19 infection in anamnesis. Results. Patients with infertility and "long-COVID" have more pronounced disorders of the reproductive function compared to women without history of COVID-19 infection, namely: a decrease in the ovarian reserve (28.8% vs. 10.0%; p<0.05), various menstrual disorders (35.0% vs. 12.5%; p<0.05), including amenorrhea, irregular menstrual cycle and luteal phase insufficiency, high prevalence of genitourinary infections (37.5% vs. 17.5%; p<0.05). Against these disorders background, patients with "long-COVID" have significantly inferior results of in vitro fertilization programs: one in five has "poor ovarian response" (21.3% vs. 2.5%; p<0.05). Clinical pregnancy was diagnosed in 22.5% of patients versus 40.0% (p<0.05), and 2 women had a pregnancy loss in the 1st trimester, while all 16 women in the comparison group carried fetuses to live birth, i.e., the live birth rate was 20.0% versus 40.0% (p<0.05). Conclusions. Determined changes in reproductive function may be directly or indirectly related to the "long-COVID" consequences, namely, a high level of stress, anxiety and depression, pernicious habits, general fatigability that leads to a sedentary lifestyle, sleep disturbances, the developing or exacerbation of somatic pathologies, among which stand out metabolic disorders and pathology of the liver and gastrointestinal tract. This study was conducted in accordance with the principles of the Declaration of Helsinki. The research protocol was approved by the Local Ethics Committee of the abovementioned institution. Informed consents of the women were obtained for participation in this study. The author declares no conflicts of interest.
The von Willebrand factor as a marker of partially controlled asthma severity in children
Authors:   Kharkiv National Medical University, Ukraine, Makieieva N.I. , Andrushchenko V.V. ,   Kharkiv National Medical University, Ukraine
Published: October 2023

Asthma is one of the most common chronic non-communicable diseases among adults and children. Recent studies have paid special attention to endothelial dysfunction in the mechanism of development and progression of asthma, on the one hand, and the occurrence of long-term consequences of endothelial damage, on the other hand. Endothelial dysfunction in the modern sense is not only a pathology of the vascular wall but also a deep, complexly organized system of disorders and compensatory and adaptive reactions that originates at the molecular genetic level. Purpose - to improve the knowledge of assessing the levels of von Willebrand factor (VWF) as a marker of endothelial dysfunction in the blood of children with partially controlled asthma. Materials and methods. 94 children participated in the study. Patients were divided into 4 groups: the Group 1 - children with mild persistent asthma (n=59), the Group 2 - moderately severe persistent asthma (n=10), the Group 3 - severe persistent asthma (n=12), and the Group 4 - control group (n=13). The study of VWF was carried out by a standard enzyme-linked immunosorbent assay (ELISA) using the Human VWF ELISA Kit. Data were analyzed using Statsoft Statistica version 8 (Tulsa, OK) and MedCalc statistical software version 17.2. Results. It was found that children with asthma had significantly increased levels of VWF in the blood serum compared to the control group. The highest levels of serum VWF were found in patients with severe asthma. Conclusions. Elevated levels of VWF indicate the presence of endothelial dysfunction. Increased levels of VWF depending on the severity of asthma indicate more severe endothelial damage in children with severe asthma. The research was carried out in accordance with the principles of the Declaration of Helsinki. The research protocol was approved by the Local Ethics Committee of the institution mentioned in the work. Informed consent of parents or their guardians was obtained for conducting research. No conflict of interests was declared by the authors.
Value of vascular cell adhesion molecule-1 and CC16 protein in bronchiolitis in young children
Authors:   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Tokarchuk N.I. , Overchuk A.A. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Published: October 2023

Given the severity of the course of bronchiolitis in young children, especially with a burdened allergic history, the search for biomarkers for early diagnosis and prognosis of the disease is urgent. Purpose - to substantiate the expediency of determining the levels of VCAM-1 and CC16 in blood serum in bronchiolitis in young children. Material and methods. The main group consisted of 34 children with bronchiolitis without a serious allergic history. The comparison group included 33 children who suffered from bronchiolitis and had a severe allergic history. The control group consisted of 25 conditionally healthy children. The clinical and laboratory examination consisted in determining the levels of VCAM-1 and CC16 in blood serum using the immunoenzymatic method. Results. The level of VCAM-1 in blood serum was established (55.5±1.12 ng/ml) in the children of the main group, while in the children of the comparison group the average value (164.9±1.1 ng/ml) of this indicator was 3 times higher, (AOR=3; 95% CI: 1.48-6.12; p<0.05). In children of the control group, the average level of VCAM-1 in blood serum was (41.6±1.32 ng/ml). Analysis of the average level of CC16 protein in blood serum revealed its significant increase in children of the main group (41.2±1.24 ng/ml) compared to the indicator of children with bronchiolitis with a severe allergic history (22.8±1.64 ng/ml ml), (AOR=1.6; 95% CI: 0.8-3.2; p<0.05). In children of the control group, the average value of the CC16 protein was within the reference values (16.2±2.43 ng/ml). Conclusions. In young children with bronchiolitis, it is advisable to determine the level of CC16 and VCAM-1 protein in blood serum. It was found that the level of SS16 protein in blood serum was significantly lower in children with a severe allergic history compared to children without a severe allergic history. On the other hand, regarding the level of VCAM-1 in blood serum, its value was significantly increased in the majority of children with severe allergic history. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
Features of COVID-19 in teenagers. Clinical cases
Authors:   Bukovinian State Medical University, Chernivtsi, Ukraine, Drutsul-Melnyk N.V. , Ivanova L.A. ,   Bukovinian State Medical University, Chernivtsi, Ukraine, Horbatiuk I.B. ,   Bukovinian State Medical University, Chernivtsi, Ukraine, Shkilniuk A.O. ,   Regional Children’s Clinical Hospital, Chernivtsi, Ukraine
Published: October 2023

Coronavirus infection in childhood is a common disease and has a number of features of the clinical course. There remain quite a lot of problems related to the variety of clinical symptoms, the severity of the course and treatment tactics in different age categories. Purpose - to study the epidemiological and clinical features of the coronavirus disease COVID-19 in adolescents compared to the younger age group in order to predict the severity of the course and determine treatment tactics. Materials and methods. A single-center retrospective open cohort study of 188 patients under the age of 18 hospitalized in the infectious disease department of the Chernivtsi Regional Children’s Clinical Hospital with a confirmed respiratory infection caused by SARS-CоV-2 was conducted. This cohort of patients is divided into 2 clinical groups. The Group I consisted of children aged 12 to 18 years (43 children), the Group II included children aged 0 to 11 years (145 patients). Results. Epidemiologically, extrafamilial sources of infection prevail in adolescence in the range of 44.2% (n=19), which is explained by greater social activity compared to children from birth to 11 years of age. For the 1st observation group, symptoms of damage to the lower respiratory tract are more characteristic, in particular, cough 81.4% (n=23), shortness of breath 51.1% (n=22), hypoxemia 14.2% (n=6) and symptoms of associated with intoxication syndrome (general weakness, asthenia 97.6% (n=42), decreased appetite, refusal to eat 74.4% (n=32), myalgia 11.6% (n=5), headache 14,2% (n=6)). The severity of the course of the coronavirus infection in adolescence is due to the dominance of lower respiratory tract lesions with the development of pneumonia in 58.2% (n=25) of cases, which required longer and more intensive treatment. Conclusions. The share of adolescents hospitalized due to COVID-19 was 22.9% (n=43), among them with a severe degree of impairment of the general condition - 16.4% (n=7), which is due to the predominance of damage to the lower respiratory tract with the development of pneumonia in 58.2% (n=25) of cases. This cohort of patients is characterized by a longer intoxication syndrome, the epidemiological role of family contacts with regard to COVID-19 decreases, and the value of unidentified non-familial sources of infection increases - 44.2% (n=19). The research was carried out in accordance with the principles of the Declaration of Helsinki. The research protocol was approved by the Local Ethics Committee of the institution mentioned in the work. Informed consent of parents or their guardians was obtained for conducting research. No conflict of interests was declared by the authors.
Assessment the impact of the torus tubarius correction by power-assisted technique on the equipressor eustachian tube function
Authors:   Shupyk National Healthcare University of Ukraine, Kyiv, Liakh K.V. ,   State Institution of Science Research and Practical Center of Preventive and Clinical Medicine State Administration Department, Kyiv, Ukraine, Kosakovskyi A.L. ,   Shupyk National University of Healthcare of Ukraine, Kyiv, Shkorbotun Ya.V. ,   Shupyk National Healthcare University of Ukraine, Kyiv,   State Institution of Science Research and Practical Center of Preventive and Clinical Medicine State Administration Department, Kyiv, Ukraine
Published: October 2023

Purpose of the research is to evaluate the impact of the torus tubarius correction by microdebrider technique on the eustachian tube function in children without middle ear pathology. Materials and methods. 115 children aged 3 to 12 years participated in the study. All children underwent endoscopic modified microdebridement adenotomy for the first time, which, in the case of tubal hypertrophy (we analyzed the frequency of this phenomenon), was combined with its correction by the microdebridement method. For the next stage of the research, we selected 19 children, who had preoperative intratympanic pressure monitoring results more than -100 daPa and who hadn’t concomitant tonsil hypertrophy (19 children). Control measurement was done on days 1 and 8-10 after the intervention. Depending on the presence/absence of concomitant hypertrophy of the torus tubarius, patients were divided into the main (11 patients) and control (8 patients) groups. The analysis was carried out using the STATISTICA program. Results. 36 patients were diagnosed with torus tubarius hypertrophy. When comparing intratympanic pressure on the 1st day after the intervention in patients of the main and control groups, a significant difference in values was found (-144.37±41.09/-95.00±27.90 daPa). On day 8-10, the intratympanic pressure data in patients of both groups did not differ from each other (-39.15±14.85 daPa in the main and -33.87±19.57 daPa in the control groups) and preoperative values in both groups. Conclusions. Hypertrophy of the torus tubarius is noted in 31.3% of patients with primary adenotomy. The microdebrider technique method of torus tubarius correction is safe in view of the equipressor function of the auditory tube. The research was carried out in accordance with the principles of the Declaration of Helsinki. The research protocol was approved by the Local Ethics Committee of the institution mentioned in the work. Informed consent of parents or their guardians was obtained for conducting research. No conflict of interests was declared by the authors.
Sosyal medya paylaşımları hisse senedi değerleri üzerinde etkili mi?
Authors: Erdoğan Şengel Demet , Yılmaz Naciye Tuba
Published: October 2023

Son yıllarda internet kullanımının yaygınlaşmasıyla birlikte bireyler bir mal veya hizmet konusunda karar verme süreçlerinde başka insanların yorumlarını da dikkate almaktadır. Benzer şekilde şirketler de müşterilerine daha iyi hizmet verebilmek ve kendi güçlü ve zayıf yanlarını görebilmek adına internetteki verileri kullanmaktadır. Bu süreçte duygu analizi, büyük verilerin yorumlanması için son yıllarda en çok tercih edilen yöntemlerden biri olmuştur. Bu çalışmanın amacı, yaygın olarak kullanılan sosyal medya platformlarından biri olan Twitter’da yapılan yazılı paylaşımların hisse senedi fiyatları üzerinde etkisinin olup olmadığını incelemektir. Bu anlamda, 1 Ocak 2016 ile 10 Nisan 2021 tarih aralığında #TSLA etiketi ile Twitter platformu üzerinde paylaşılan yazılı ifadelerin derin öğrenme yöntemlerinden biri olan BERT modeli ile duygu analizi gerçekleştirilmiş ve paylaşımların pozitif veya negatif anlama sahip olup olmadığı tespit edilmiştir. Paylaşım yapılan günün ardından gelen günde yorumların olumlu veya olumsuz olması durumuna göre TSLA hisse senedi değerinin azalma veya artış gösterip göstermediğinin tahmin edilmesi Neural Prophet ile gerçekleştirilmiştir. Sonuç olarak sosyal medya paylaşımlarının bireylerin hisse senetlerini alış ve satış kararları üzerinde etkili olduğu sonucuna varılmıştır.
Dijitalleşmenin çevresel belirsizlik ortamında çevikliğe etkisi*
Authors: Güler Ümran , Sönmez Rukiye
Published: October 2023

Dijitalleşme, günümüzde rekabet avantajının elde edilmesinde önemli bir araç olarak görülmesine rağmen firmaların dijitalleşip dijitalleşmemek konusunda bir ikileme sahip olduğu görülmektedir. Özellikle belirsizlik ortamında, rekabetçi çevrede yetkin ve esnek olabilmek, hızla yanıt verebilmek yani çevik olmak beklenir hale gelmiştir. Bu nedenle bu araştırmada dijitalleşme ve çevresel belirsizliğin çevikliği etkileyip etkilemediğini sorgulamaktadır. Araştırmanın örneklemi, dijitalleşme sürecini çevresel koşullar altında hızlandırmak zorunda kalan bankacılık sektörü olarak belirlenmiştir. Örneklemden anket yöntemi ile 262 kullanılabilir ankete ulaşılmıştır. Değişkenler arasındaki etkileri görebilmek için modeller varyans temelli eşitlik modeli ile sınanmıştır. Modellerin sınanmasında SmartPLS 3.4 istatistik paket programı kullanılmıştır. Sonuç olarak dijitalleşme, çevresel belirsizlik üzerinden çevikliğin yetkinlik, esneklik, cevap verme ve hız boyutlarını etkilediği bulunmuştur.
Makyavelizmin iş tatminine etkisinde örgütsel öğrenmenin rolü
Authors: Çetin Zeki
Published: October 2023

Yüksek iş tatminine sahip çalışanlar, örgütsel performans ve rekabet üstünlüğü için temel dinamiklerdendir. Bu bağlamda çalışanların iş tatminini artıracak unsurların tespit edilmesi ve bu unsurlara yönelik örgütsel stratejiler geliştirilmesi gerekmektedir. Mevcut araştırmada makyavelizmin iş tatminine etkisinde örgütsel öğrenmenin aracı rolünün belirlenmesi amaçlanmıştır. İstanbul’daki üniversitelerde çalışan 385 katılımcının örneklem olarak alındığı araştırmada, öz bildirim ölçekleri kullanılarak veri toplanmıştır. Verilerin analizinde Smart PLS 4 ile kısmi en küçük kareler yöntemi kullanılarak yapısal eşitlik modellemesi kurulmuştur. Sonuçlar makyavelizmin hem iş tatminini hem de örgütsel öğrenmeyi azalttığını, örgütsel öğrenmenin ise iş tatminini artırdığını ortaya koymuştur. Bununla birlikte makyavelizmin iş tatminine etkisinde örgütsel öğrenme aracı rol üstlenmiştir. Araştırma bulgularına dayanarak Makyavelizmin iş tatmini üzerindeki olumsuz etkilerinin engellenebilmesi için öğrenen örgüt kültürünün sağlamlaştırılmasına yönelik stratejiler oluşturulması gerektiği düşünülmektedir.
The effect of inflation on the budget deficit in Turkiye: Evidence from cointegration test with multiple structural breaks
Authors: Demir Fatih
Published: October 2023

In determining the relationship between the budget deficit and inflation in literature, the effect of the budget deficit on inflation has been widely focused on. Although the impact of inflation on the budget deficit is highly significant, there has been a lack of sufficient research on this matter. There are two different views put forward on this issue. The first of these is the Tanzi effect; argues that an increase in inflation will cause a real decrease in tax revenues and therefore the budget deficit will increase. The opposing view is known as the Patinkin effect and suggests that the budget deficit will decrease because inflation will reduce real public expenditures. In this study, the effect of inflation on the budget deficit was investigated for the period 1960 2022 in Turkiye. Considering that the data period may contain many structural breaks, Carrion-i-Silvestre unit root test and Maki cointegration test were used. The years 1995, 2002, 2007, 2011 and 2018 were determined as the break dates. According to the results, the inflation rate has an increasing effect on the budget deficit in the period when there is no break. The overall result did not change when the effect of breaks was included. With these findings, it can be said that the Tanzi effect is more dominant in Turkiye.
Role of women in today’s society and the inequality in genders
Authors: Masood Rameen
Published: October 2023

The main method used was a survey which around 350 working women from multiple countries including Pakistan, Turkey, and Lebanon responded to, regarding different aspects of their professional ideologies and observations. Extensive secondary research was employed to explore the issues of role of women in society, sexual harassment faced by women in their homes and workplaces, and the magnitude of gender inequality in developing versus developed countries. The main findings concluded that gender inequality is a long-standing hurdle in the path of women. It exists at all levels in the society and is not limited to a single culture or country. The gender inequality is not the only barrier faced by women in their journeys; however, it may pave way for other obstacles like sexual harassment. The measures presently taken to combat the negative impacts of gender inequality on women are inadequate and require more thought and better implementation for them to prove fruitful in eradicating the issue.
Aylak zamanı enküçükleyen tur oluşturma problemlerinin geri izleme yöntemi ile çözümüne ilişkin bir yazılım geliştirme uygulaması
Authors: İnanç Şahin , Sezen Hayrettin Kemal
Published: October 2023

İşletmelerde gün geçtikçe artan rekabet koşullarında küçük avantajlar bile önemli olabiliyorken lojistik de her geçen gün daha da büyük bir öneme sahip olmaktadır. İşletmelerin giderlerinin önemli bir kısmını lojistik oluşturmaktadır. Bu çalışma karayolunda yolcu taşıması yapan bir lojstik şirketinin araçlarının seferleri arasında beklemelerine ilişkin aylak zaman toplamını en küçük kılacak şekilde, optimal çözümü garanti eden bir (kesin, exact) yöntemle çözümü gerçekleştirilmiştir. Çözüme yönelik C# programlama dili ile bir yazılım geliştirilmiştir. Problemin çözümüne ilişkin yöntem olarak Dal ve Sınır tekniğinin Geri İzleme yaklaşımı kullanılmıştır. Kullanılan bu yöntem; tam sayıma göre sayımlamayı azaltma özelliğine sahiptir.
La representación social del amor en el contexto de la posmodernidad pandémica
Authors: Hernández Zuluaga Germán Darío , Paradela López Miguel , Blanco Sánchez Miguel
Published: October 2023

A través del presente artículo se desarrolla un diálogo de autores con el propósito de comprender la representación social del amor en el contexto de la posmodernidad pandémica, desarrollando fundamentalmente aspectos como la resignificación, el amor, las tipologías del amor y la posmodernidad, con el propósito de indagar en las posibles transformaciones en la representación social del amor a partir de la pandemia por el Covid-19. El artículo se desarrolló a partir de un rastreo documental con un alcance hermenéutico en el que se encuentran bases para comprender cambios recientes en la manera de amar. En este sentido, se concluye que las reflexiones en torno a las representaciones sociales del concepto del amor en la posmodernidad pandémica, toman sentido en la medida en que se comprende que la pandemia acontece en un escenario de profundos cambios sociales mediados, entre otros factores, por nuevos modelos relacionales, característicos de la posmodernidad, permeados por cuestiones como las nuevas tecnologías de la comunicación y la información y sus implicaciones en la forma de establecer contacto con otras personas, lo cual se acentuó, especialmente, con la aparición de la pandemia; en este sentido, se considera que el amor, como se ha conocido en los últimos tiempos, ha sufrido un cambio como consecuencia de la pandemia en la posmodernidad.
La migración Sur-Sur hacia Chile: perspectivas, análisis y elementos de discusión
Authors: Rodríguez-Torrent Juan Carlos , Vargas Callegari Rodrigo
Published: October 2023

La comprensión de las migraciones internacionales constituye uno de los principales retos de las ciencias sociales en la actualidad, como un efecto inherente al modelo capitalista posfordista, dentro de un contexto histórico poscolonial. El marco de reflexión se ubica en el ámbito de lo nacional y transnacional, e involucra procesos, coproducciones y vínculos entre los Estados-nación, lo que implica el cruce de límites y la radicación de la cultura en micro-espacios, principalmente urbanos, en territorios ajenos. En tres décadas el reto ha pasado desde el problema de la identidad, las relaciones asimétricas con otros y la asimilación, al tema del reconocimiento y los derechos de ciudadanía, trascendiendo la dicotomía entre origen y destino, así como entre centro y periferia. El viaje, los intercambios, las redes, la instalación, y servicios que se abren y cierran, son sólo algunas perspectivas de una constelación de problemas y de objetos que complejizan su reflexión. Este trabajo, presenta algunos resultados analíticos y teóricos del proceso migratorio Sur-Sur, a partir de un permanente flujo de recepción de migrantes en Chile, dentro de un esquema político neoliberal y refractario en lo económico, que dificulta la capacidad de leer los impactos en las poblaciones y comunidades.
Reconfiguración sociometabólica del agua: tensiones, actores y estrategias colectivas hacia la justicia socio ambiental en el Istmo de Tehuantepec, Oaxaca, México
Authors: Ramírez Antonio Karen Guadalupe , Méndez García Elia María Del Carmen , Fuente Carrasco Mario Enrique
Published: October 2023

El objetivo de este artículo es analizar las reconfiguraciones de los flujos de agua provocados por la acción de actores como el Estado y las comunidades que generan situaciones de injusticia socioambiental en una comunidad del Istmo de Tehuantepec en el estado de Oaxaca, al sureste de México; así como las estrategias desde el sujeto comunitario para afrontar los efectos adversos. Se utilizaron categorías como configuraciones sociometabólicas, conflictos ecológico-distributivos, fractura sociometabólica, justicia socioambiental, coproducción y horizontes de deseo. Se desarrolló una investigación cualitativa de mayo de 2020 a octubre de 2022 con técnicas de observación participante, entrevistas en profundidad semiestructuradas y un taller de mapeo comunitario con autoridades agrarias; así como una profunda revisión documental. Tras hacer la reconstrucción histórica de la reconfiguración sociometabólica del agua, se encontró que las tensiones y gestiones territoriales han provocado transformaciones en el territorio y situaciones de injusticia ambiental para los habitantes de San Pedro Comitancillo. Se documentan las motivaciones del sujeto comunitario para emprender acciones sobre su espacio que mitigan los impactos de una fractura socio metabólica, las cuales se relacionan con la reconfiguración del territorio cultivable que permite la adaptación a nuevas condiciones ambientales y la integración al mercado. Se identifican cambios en las esferas económica, social y cultural Se buscó una articulación interdisciplinaria que permita el engranaje de los componentes tangible e intangible del metabolismo social y que profundice en el sentido de las acciones de los actores. Este trabajo permite comprender las reconfiguraciones dinámicas de espacios para la reproducción material y simbólica de la vida.
El liderazgo y cuidado femenino en los asentamientos humanos de desarrollo incompleto en Cali
Authors: Castillo-Valencia Maria , Bahos-Mendoza Diana Marcela
Published: October 2023

Esta investigación analiza el papel de las mujeres como agentes determinantes en el desarrollo de siete asentamientos humanos de desarrollo (AHDI) incompleto en las zonas de Ladera y Oriente en Cali.  Usamos un enfoque feminista del cuidado  para mostrar cómo  el trabajo individual y colectivo de las mujeres en los lugares a donde llegan no sólo mejora sus condiciones de vida, sino las de su familia y de todos sus habitantes. Usando datos del Censo Nacional de Población y Vivienda (CNPV) del 2018 y del SISBÉN IV del 2020 se describen sus precarias condiciones socioeconómicas para luego, identificar cómo ellas logran trascender en su papel de cuidadoras en la esfera privada del hogar a la pública representada por la comunidad, con información proveniente de entrevistas semiestructuradas y grupos focales.  Clasificada en cuatro ejes temáticos: características sociodemográficas, memorias de asentamiento y reubicación, escenarios de participación y obstáculos, y percepción y violencias basadas en género. Se resalta  su trabajo colectivo, sus luchas y las estrategias que emplean para lograr mejoras en el lugar donde residen, identificando diferencias entre asentamientos de ladera y oriente en la forma en que las mujeres acceden a los servicios públicos y en los logros obtenidos Aunque existen diversos estudios sobre el trabajo comunitario en los AHDI, este trabajo busca diferenciar con enfoque de género,  el rol y agencia de la mujer frente al de los hombres, en estos procesos que llevan a  un mejor hábitat en el asentamiento.
Estudio etnoeconómico de los inmigrantes venezolanos en la comuna 6 de Cali
Authors: Jiménez Restrepo Diana Marcela , Maya Velasco Alexandra
Published: October 2023

El objetivo de este documento es el de comprender, mediante el enfoque de las economías étnicas, cómo los emprendimientos de los inmigrantes venezolanos en la comuna 6 de Cali han contribuido a su integración social y laboral en esta zona y determinar cómo el capital cultural y social han ayudado en ello. Para lograrlo, se llevó a cabo una metodología mixta, mediante la cual se encuestaron y entrevistaron a 19 inmigrantes venezolanos y 2 colombianos retornados para evaluar su situación social, económica y laboral, encontrándose que en la comuna 6 de Cali se ha desarrollado una economía étnica mixta en la que la interacción social entre coétnicos y el intercambio cultural con nacionales son fundamentales para superar las dificultades del mercado laboral local, la xenofobia y la discriminación que enfrentan como minoría social. Aunque los inmigrantes en esta zona siguen expuestos a condiciones laborales precarias, los valores comunales, como la solidaridad y el compañerismo, les permiten contribuir a su integración vertical en la sociedad nacional y, según su percepción, a mejorar sus condiciones de vida. La originalidad de este trabajo parte desde el diseño para el levantamiento de información de inmigrantes venezolanos en Cali junto con el respectivo análisis que aquí se desarrolla.
Framing the Fragment, from Parchment to Pixel
Authors: Davis Lisa Fagin
Published: October 2023

Applying the theme of Use and Reuse to the practice of manuscript fragmentation, this essay will address the material and ontological “framing” of leaves of dismembered manuscripts. Manuscript leaves undergo multiple types of “framing” as they journey from their medieval haptic origins to the digital realm. A parchment leaf begins as the hide on an animal’s skeletal framework, a fleshly origin whose shape is permanently imprinted on the folio. That hide is then stretched on a parchmenter’s frame for scudding and preparation for trimming and writing. The book’s binding is another framelike container that holds the leaf and provides its spatial boundaries. If a manuscript is dismembered, the leaf may find itself contained not in a binding but in a matte, the matte then framed for presentation on a wall. As we move into the digital space, images must be themselves contained in the frame of a viewer. What can we make of these various transformations and the frames that contain and constrain them?
FLIM-FRET analyses with mCitrine-mScarlet-I transiently expressed in N. benthamiana v1
Authors: not provided Elena-Kristin.Petutschnig
Published: October 2023

Elucidating protein-protein interactions is crucial for our understanding of molecular processes within living organisms. Microscopy-based techniques can detect protein-protein interactions in vivo at the single cell level and provide information on their subcellular location. Fluorescence Lifetime Imaging Microscopy (FLIM) - Förster resonance energy transfer (FRET) is one of the most robust imaging approaches, but it is still very challenging to apply this method to proteins which are expressed under native conditions. We have established a novel combination of fluorescence proteins (FPs), mCitrine and mScarlet-I, which is ideally suited for FLIM-FRET studies of low abundance proteins expressed from their native promoters in stably transformed plants. The donor mCitrine displays excellent brightness in planta, near-monoexponential fluorescence decay and a comparatively long fluorescence lifetime. In this protocol we describe FLIM-FRET experiments for control proteins in Nicotiana benthamiana, namely mCitrine (donor only control), mScarlet-I (acceptor only control) and a mCitrine-mScarlet-I fusion protein (FRET control).
FLIM-FRET analyses with mCitrine / mScarlet-I -tagged chitin receptors stably expressed in A. thaliana v1
Authors: not provided Elena-Kristin.Petutschnig
Published: October 2023

Elucidating protein-protein interactions is crucial for our understanding of molecular processes within living organisms. Microscopy-based techniques can detect protein-protein interactions in vivo at the single cell level and provide information on their subcellular location. Fluorescence Lifetime Imaging Microscopy (FLIM) - Förster resonance energy transfer (FRET) is one of the most robust imaging approaches, but it is still very challenging to apply this method to proteins which are expressed under native conditions. We have established a novel combination of fluorescence proteins (FPs), mCitrine and mScarlet-I, which is ideally suited for FLIM-FRET studies of low abundance proteins expressed from their native promoters in stably transformed plants. The donor mCitrine displays excellent brightness in planta, near-monoexponential fluorescence decay and a comparatively long fluorescence lifetime. In this protocol, we describe FLIM-FRET experiments for CERK1-mCitrine and LYK5-mScarlet-I or CERK1-Scarlet-I in stably transformed Arabidopsis lines.
Phytophthora pluvialis culture cultivation, maintenance, and detached needle assay protocol v1
Authors: Eccersall Sophie , Seesom Vinson Leann , McDougal Rebecca , Meisrimler Claudia-Nicole
Published: October 2023

Phytophthora pluvialis is an oomycete that primarily infects Pinus radiata and Pseudotsuga menziesii causing the destructive foliar disease red needle cast (RNC). Recent observations show that P. pluvialis can also infect western hemlock inducing resinous cankers. High-throughput and reproducible infection assays are integral to find key information on tree health and oomycete pathogenicity. In this protocol, we describe the propagation and spore induction of P. pluvialis, followed by detached needle assays for verification and quantification of virulence of P. pluvialis in P. radiata needles. These needle assays can be employed for high-throughput screening of tree needles with diverse genetic backgrounds.In downstream analysis, Quantitative PCR (qPCR) was utilised to assess relative gene expression, as exemplified by candidate RxLR effector protein PpR01.Additional techniques like RNA sequencing, metabolomics, and proteomics can be combined with needle assays and can offer comprehensive insights into P. pluvialis infection mechanisms
An assessment of the reliability of CFRP composites used in nodes of friction after impact of UV-A impacts and thermal shocks
Authors: Krzyzak Aneta , Racinowski Damian , Szczepaniak Robert , Kosicka Ewelina
Published: October 2023

This article describes the results of tribological research into epoxy-based composites reinforced with carbon fiber. The composites were subjected to accelerated tests simulating a semi-annual influence of environmental conditions of an elevated temperature, precipitation in combination with an influence of UV-A radiation of 0.83 W/m 2 as well as cyclic thermal shocks causing a leap temperature difference of 116.5°C. The process of friction was conducted in conditions of dry friction and wet friction in the presence of water. The authors found a positive influence of a two-month impact of environmental conditions upon increasing wear resistance. They found a reduction in weight in conditions of friction with water. At the same time, a reliability analysis for the same boundary conditions showed an increased risk of critical composite damage. The article indicates areas of safe exploitation of composites and areas of the necessity of withdrawing composites from further exploitation under the assumed environmental and tribological loads.