bluuebunny/crossref_metadata_2025 huggingface dataset:
LiteHash: Hash Functions for Resource-Constrained Hardware
Authors: Achar Sagar Dev , P Thejaswini , Nandi Sukumar , Nandi Sunit
Published: July 2024

The global paradigm shift toward edge computing has led to a growing demand for efficient integrity verification. Hash functions are one-way algorithms which act as a zero-knowledge proof of a datum’s contents. However, it is infeasible to compute hashes on devices with limited processing power and memory. Hence, we propose four novel
LiteHash
functions which are architecturally similar to SHA-512 yet simpler. By using various approximation techniques, our implementations reduce the computational costs of digesting a message into a hash. On validating our proposed designs using the NIST PRNG Test Suite, we observe SHA-512 equivalent cryptographic security while satisfying all desired hash function property requirements. We observe a minimum of 9.41% reduction in area, 20.47% reduction in power, and 22.05% increase in throughput. Our designs offer a throughput of up to 2 Gbps while reducing area and power by a maximum of 16.86% and 32.48%, respectively.
LiteHash
functions also support the computation of the entire SHA-2 family of hash functions (SHA-224/256/384/512) with minor architectural modifications.
Lactate promotes fatty acid oxidation by the tricarboxylic acid cycle and mitochondrial respiration in muscles of obese mice
Authors: Park Sol-Yi , Jung Su-Ryun , Kim Jong-Yeon , Kim Yong-Woon , Sung Hoon-Ki , Park So-Young , Doh Kyung-Oh , Koh Jin-Ho
Published: July 2024

Lactate administration post-exercise promotes triglyceride content loss in skeletal muscles (SKMs) and reduced body weight. Lactate enhances fatty acid oxidation in the SKMs of high-fat diet (HFD)-fed mice due to enhanced mitochondrial oxygen consumption. In addition, lactate restores the malate-aspartate shuttle, which is reduced by a HFD, and activates the tricarboxylic acid cycle (TCA) cycle in SKMs. Interestingly, supraphysiological lactate facilitates uncoupling protein-3 expression through NADH/NAD+, which is enhanced under high-fat levels in SKMs.
Glutamine metabolism improves left ventricular function but not macrophage-mediated inflammation following myocardial infarction
Authors: Mouton Alan J. , Aitken Nikaela M. , Morato Jemylle G. , O’Quinn Katherine R. , do Carmo Jussara M. , da Silva Alexandre A. , Omoto Ana C. M. , Li Xuan , Wang Zhen , Schrimpe-Rutledge Alexandra C. , Codreanu Simona G. , Sherrod Stacy D. , McLean John A. , Stanford Joshua K. , Brown Jordan A. , Hall John E.
Published: July 2024

Glutamine metabolism is altered in both infarct macrophages and the remote left ventricle (LV) following myocardial infarction (MI). Supplemental glutamine improves LV function following MI while inhibiting glutamine metabolism with BPTES worsens LV function. Supplemental glutamine or BPTES does not impact macrophage immunometabolic phenotypes after MI.
Involvement of the gut microbiota in cancer cachexia
Authors: VanderVeen Brandon N. , Cardaci Thomas D. , Bullard Brooke M. , Madden Michael , Li Jie , Velazquez Kandy T. , Kubinak Jason L. , Fan Daping , Murphy E. Angela
Published: July 2024

Cancer cachexia, or the unintentional loss of body weight in patients with cancer, is a multiorgan and multifactorial syndrome with a complex and largely unknown etiology; however, metabolic dysfunction and inflammation remain hallmarks of cancer-associated wasting. Although cachexia manifests with muscle and adipose tissue loss, perturbations to the gastrointestinal tract may serve as the frontline for both impaired nutrient absorption and immune-activating gut dysbiosis. Investigations into the gut microbiota have exploded within the past two decades, demonstrating multiple gut-tissue axes; however, the link between adipose and skeletal muscle wasting and the gut microbiota with cancer is only beginning to be understood. Furthermore, the most used anticancer drugs (e.g. chemotherapy and immune checkpoint inhibitors) negatively impact gut homeostasis, potentially exacerbating wasting and contributing to poor patient outcomes and survival. In this review, we 1) highlight our current understanding of the microbial changes that occur with cachexia, 2) discuss how microbial changes may contribute to adipose and skeletal muscle wasting, and 3) outline study design considerations needed when examining the role of the microbiota in cancer-induced cachexia.
Does Lifestyle Affect Isotretinoin Tolerance in Patients with Acne? A Retrospective Cohort Study
Authors: Lewandowska Julia , Kapała Jan , Placek Waldemar , Owczarczyk-Saczonek Agnieszka
Published: July 2024

Isotretinoin (ISO) is a synthetic retinoid approved for the treatment of acne vulgaris. Despite satisfactory results, it is still controversial due to numerous adverse events (AE). However, there is a lack of literature examining the factors affecting the frequency and severity of these AEs. Lifestyle patterns may have an impact as they have the greatest impact on a person’s overall health. The aim of the research is to evaluate the impact of lifestyle patterns on the occurrence and severity of systemic ISO AEs in patients suffering from acne. A retrospective cohort study was conducted using a database of collected information about adults diagnosed with acne and treated with systemic ISO. Patients were divided into several groups according to their smoking status (nonsmokers, smokers), BMI (normal weight, overweight), physical activity (active, inactive), and compliance with the Mediterranean diet (adherent, not adherent). Considering all mentioned lifestyle factors, responders were categorized into healthy and unhealthy lifestyle groups. 124 adults with acne, undergoing systemic ISO therapy, made up the study group. There were generally no significant differences in patient characteristics across groups (P > 0.05). Almost all classes and subclasses of AEs showed no significant differences in groups (P > 0.05). ISO is still the most effective drug in the treatment of acne and despite the AEs, therapy should not be abandoned. Moreover, lifestyle factors such as physical activity, smoking, and eating habits do not affect the incidence and severity of AEs, which proves the safety of ISO regardless of unhealthy habits.
Characterizing the Tumor Microenvironment and Its Correlation with cDC1‐Related Gene Expression in Gastric Cancer
Authors: Han Song-Hee , Ju Mi Ha
Published: July 2024

Background and Aim. Gastric cancer (GC) remains a significant global health challenge. The role of conventional type 1 dendritic cells (cDC1s) in the tumor microenvironment and their association with cancer prognosis is gaining attention. This study aims to evaluate the prognostic value of cDC1‐related genes (CD141, XCR1, CLEC9A, CADM1, and BTLA) in GC and explore their underlying biological mechanisms. Materials and Methods. We analyzed RNA‐seq data from the Cancer Genome Atlas (TCGA‐STAD) and Gene Expression Omnibus (GEO) datasets, focusing on five cDC1‐related genes. The cDC1‐related signature was defined and divided into high and low expression groups. We employed gene set variation analysis (GSVA) for oncogenic signaling pathways and conducted comprehensive statistical analyses, including Kaplan–Meier and Cox proportional hazards models. Results. The high cDC1‐related gene signature group was associated with poorer overall and disease‐free survival in the TCGA‐STAD cohort. Significant differences in CD8+ T cell infiltration and cytotoxic capabilities were observed between high and low CDC1‐related signature groups. The study also revealed a strong correlation between CDC1‐related signature and increased expression of immune checkpoint proteins and oncogenic pathways, suggesting a complex immunosuppressive tumor microenvironment. Conclusions. Our findings indicate the potential of the cDC1‐related signature as a prognostic marker in GC, offering insights into the tumor–immune interplay. The study underscores the importance of cDC1s in shaping the tumor microenvironment and their influence on patient prognosis in GC. These results may contribute to the development of novel therapeutic strategies targeting the immune microenvironment in GC.
Exploring Solution Strategies for Volterra Integro‐Differential Lane–Emden Equations in Astrophysics Using Haar Scale 3 Wavelets
Authors: Kumar Ratesh , Bakhtawar Sabiha , Emadifar Homan
Published: July 2024

The current research introduces a novel approach to address the computational challenges associated with solving the Lane–Emden‐type equations by transforming them from their conventional differential form to the corresponding integro‐differential form. These equations have wide‐ranging applications in physical sciences, including modeling diffusion phenomena and thermal gradients. We utilize the Volterra integro‐differential (VID) form to resolve computational challenges due to singularity issues. Through the Scale 3 Haar Wavelet (S3‐HW) algorithm, we transform the VID equations into algebraic form and obtain solutions using the Gauss‐elimination method. The quasilinearization technique is implemented whenever a nonlinearity is encountered. Comparative analysis against various techniques demonstrates the superior accuracy and efficiency of our method. Despite challenges such as the discontinuity of Scale 3 Haar Wavelets and singularity issues of Lane–Emden‐type equations, our algorithm paves the way for extending its application to a wide range of physical problems.
Evaluation of Efficacy, Safety, and Withdrawal Period of Enrofloxacin (ENR) in Black Rockfish (Sebastes schlegelii) for Possible Treatment of Bacterial Diseases Caused by Streptococcus iniae and Aeromonas salmonicida
Authors: Bae Jun Sung , Lee Chae Won , Yang Chan Yeong , Jeong Eun Ha , Chae Young-Sik , Lee Ji-Hoon , Park Sujin , Seo Jung-Soo , Kwon Mun-Gyeong , Kim Hyo In , Park Kwan Ha
Published: July 2024

Although enrofloxacin (ENR) is an important pharmaceutical in aquaculture for its excellent efficacy, it is mostly utilized through an off‐label prescription system. Thus, the optimal clinical use regimen is not well‐established. A series of studies was performed to obtain pharmacological and safety data needed for the use of ENR, a synthetic fluoroquinolone, in treating bacterial diseases occurring in black rockfish, Sebastes schlegelii. Minimal inhibitory concentrations showed that ENR exerts a potent in vitro efficacy inhibiting the growth of several pathogenic bacteria at concentrations less than 1 mg/L. ENR with oral doses of 5 mg/kg and up for 1 week significantly prevented mortality of black rockfish infected with Streptococcus iniae and Aeromonas salmonicida, demonstrating in vivo efficacy. Complete protection was obtained against these two bacteria by 10 mg/kg ENR in this in vivo infection trial. Black rockfish did not exhibit any severe abnormal signs by ENR up to 20 mg/kg when assessed by basic haematoclinical and histopathological parameters. Efficacy and toxicity study results collectively suggest that oral dose of 10 mg/kg ENR is optimal to treat bacterial infections. Through muscle residue analyses, the required withdrawal period for safe fish consumption was found to be approximately 90 days by measuring clearance of ENR and its metabolite ciprofloxacin (CIP). This study provides necessary information for the use of ENR to treat two frequently‐occurring bacterial infections, Streptococcus iniae and Aeromonas salmonicida in black rockfish. Obtained data can be the basis for an official guideline for aquacultural ENR use.
Study of the Mechanical Behavior of Retaining Structures and Adjacent Buildings during the Excavation of Deep and Long Pits
Authors: Wang Dongsheng , Wang Shuhong , Zhang Ze , Ding Peng
Published: July 2024

Excavation of deep and long pits can cause strata deformation and settlement of adjacent buildings. The excavation of a deep foundation pit of a nearby building in Zhengzhou City, China, is taken as the research object. The foundation of the foundation pit is an independent foundation under the column, the depth of the foundation pit is 24.5∼26.3 m, and the support form of an underground continuous wall and four internal supports is adopted. The stress and deformation characteristics of retaining structures during the construction of deep and long pits and their impact on the deformation of nearby buildings were studied through on‐site monitoring and finite element simulation. The analysis focuses on the effects of different excavation stages on adjacent structures, with the main conclusions as follows. After excavation, the underground continuous wall far from the building undergoes a “rotating‐kick” displacement, while the underground continuous wall on the side near the building only shows a “kick” displacement. Adjacent buildings have little influence on the distribution of shear force and bending moment of the underground continuous wall of the foundation pit. The first horizontal strut experiences pressure, gradually shifting to tension as excavation continues, with the maximum stress reaching 3.6 × 103 kN. The second, third, and fourth horizontal struts mainly bear compressive forces, increasing with the depth of the struts. The building primarily undergoes settlement in the early stages of deep and long pit excavation. As the excavation progresses, the building points near the pit begin to bulge, but the building corners far from the pit continue to show settlement. During the excavation of the deep and long pit, the differential settlement of the long side of the nearby building first decreases and then increases, and the differential settlement of the short side changes from a slight decrease to a significant increase. The settlement evolution of the building is as follows: overall settlement ⟶ overall uplift ⟶ uplift of the side of the building near the pit ⟶ outward tilting away from the pit. The findings may provide references for designing, constructing, and operating deep and large foundation pits.
Integrating Demand Response with Unit Commitment in Insular Microgrid considering Forecasting Errors and Battery Storage
Authors: Swami Rekha , Gupta Sunil Kumar , Bansal R. C.
Published: July 2024

In this paper, DR programs are integrated with the unit commitment economic dispatch model for a single day to lower total operating costs for an insular microgrid. The proposed model takes into account the forecasting errors associated with wind, solar, and load demands. A new combined DR program is presented to enhance microgrid operation and financial effectiveness, benefiting microgrid consumers. The price elasticity and consumer profit are the foundation for DR modeling. The optimization problem is developed as mixed‐integer nonlinear programming (MINLP) and solved using GAMS software. For the case study, an insular microgrid consisting of two microturbines, a wind turbine, solar photovoltaic, and battery storage is considered. Optimization is carried out under both with and without the DR program. The outcomes show that by implementing TOU and DLC DR programs, the operating cost is reduced by 13.55% and 9.68%, respectively. While consumers experience a financial loss in TOU‐DR, they earn profit in DLC‐DR. Therefore, a combination of the two, i.e., TOU + DLC DR, is proposed, reducing operating costs by 10.73% while increasing profit for users. The suggested approach benefits the microgrid operator as well as its users, encouraging the construction and operation of insular microgrids in rural or isolated areas.
Nonunique Fixed‐Point Results in a General Setting with an Application
Authors: Ahmad Haroon , Younis Mudasir , Koksal Mehmet Emir , Lateef Durdana
Published: July 2024

This manuscript’s main goal is to examine the presence of fixed points in the context of controlled metric‐type spaces utilizing rational type Ćirić‐contractive conditions. We provide appropriate examples to support the validity and superiority of our conclusions. In the context of extended b‐metric space, b‐metric spaces, and standard metric spaces, our findings extrapolate and expand a number of well‐known research findings. As an application of our findings, we prove that a second‐order differential equation has a solution. The paper ends with a few open problems for further investigation of additional dimensions within this framework.
Optimization of Pipeline Leakage Detection System in Utility Tunnel Based on Finite Element Method
Authors: Liu Yongli , Wang Jiao , Xue Tiantian , Xiao Henglin , Yang Yanshuang
Published: July 2024

In addressing the problems of delayed detection, inefficient identification, and coverage blind spots in optical fiber‐based pipeline leakage detection within pipe galleries, this study proposes a leakage detection strategy utilizing distributed optical fiber temperature measurement technology. Finite element method is employed to analyze the temperature influence radius around the pipeline leakage hole and a model test is conducted as a validation. The results show that the temperature field image at the leakage site is elliptical, influenced by temperature differences between ambient and liquid. An increase in this temperature difference accelerates changes in the temperature field’s range. Adjustments to the optical fiber’s winding angle and pitch demonstrated that an optimal pitch is 1/24 of the pipeline’s length, with a 45° winding angle. This configuration maximizes the optical fiber’s distribution in detection while maintaining its cost‐effectiveness. When the leakage site is constant, and only the winding mode is altered, it is observed that when the ambient temperature exceeds the liquid temperature in the pipeline, the temperature of the escaping liquid impacts the temperature‐measuring fiber due to gravity, registering approximately 2°C higher than the temperature measured directly at the leakage site. The temperature anomaly from field diffusion is significantly less than that caused by the water flow from the leakage impacting the fiber due to gravity. Conversely, when the ambient temperature is lower than the pipeline’s liquid temperature, the opposite occurs. These research findings offer a novel approach for distributed detection in water supply and drainage pipeline leakage.
Reproductive Outcomes of Dual Trigger versus hCG Alone in Women Undergoing In Vitro Fertilization with Fresh Embryo Transfer Cycles
Authors: Guner Fatma Ceren , Ozekinci Murat , Mendilcioglu Ibrahim Inanc , Kasabali Zeliha
Published: July 2024

Aim. To investigate the effect of the triggering method on the results of fresh embryo transfer in patients who underwent gonadotropin‐releasing hormone antagonist cycles. Methods. The study was conducted retrospectively at a university‐based tertiary reproductive center. The sample consisted of a total of 295 patients, of whom 111 were in the human chorionic gonadotropin (hCG) trigger group and 184 were in the dual trigger group. The main outcome measure of this study was the live birth rate, and secondary outcomes were the implantation rate, clinical pregnancy rate, miscarriage rate, and good‐quality embryo rate. Results. Patient demographics and baseline characteristics did not significantly differ between the dual and hCG trigger groups. The results also indicated statistically nonsignificant differences between the two groups in terms of the number of oocytes retrieved (p > 0.05), the number of mature oocytes (p > 0.05), and the fertilization rate (p > 0.05). The number of good‐quality embryos (p = 0.002) was higher in the dual trigger group compared with the hCG trigger group. However, the rates of clinical pregnancy and live births did not significantly differ between the groups (p > 0.05). Conclusions. Although the number of total and high‐quality embryos obtained was higher in the dual trigger group, there were no significant differences between the two groups in terms of pregnancy outcomes. The fresh embryo transfer yielded similar rates of implantation and live births in both trigger groups.
Geographic Access to Fertility Counseling among Adolescent and Young Adult Women with Cancer in North Carolina
Authors: Rodriguez-Ormaza Nidia , Anderson Chelsea , Baggett Christopher D. , Delamater Paul L. , Troester Melissa A. , Wheeler Stephanie B. , Wardell Alexis C. , Deal Allison M. , Smitherman Andrew , Mersereau Jennifer , Baker Valerie L. , Nichols Hazel B.
Published: July 2024

Background:

Fertility counseling is recommended for adolescent and young adult women facing gonadotoxic cancer therapy. However, fertility care is subspecialized medical care offered at a limited number of institutions, making geographic access a potential barrier to guideline-concordant care. We assessed the relationship between geographic access and receipt of fertility counseling among adolescent and young adult women with cancer.

Methods:

Using data from the North Carolina Central Cancer Registry, we identified women diagnosed with lymphoma, gynecologic cancer, or breast cancer at ages 15 to 39 years during 2004 to 2015. Eligible women were invited to complete an online survey on various topics, including fertility counseling. Geographic access was measured, using geocoded addresses, as vehicular travel time from residence to the nearest fertility clinic available at diagnosis. Multivariable regression models were used to examine the association between travel time and receipt of fertility counseling by provider type: health care provider versus fertility specialist.

Results:

Analyses included 380 women. The median travel time to a fertility clinic was 31 (IQR: 17–71) minutes. Overall, 75% received fertility counseling from a health care provider and 16% by a fertility specialist. Women who lived ≥30 minutes from a clinic were 13% less likely to receive fertility counseling by a health care provider (prevalence ratio: 0.87; 95% confidence interval, 0.75–1.00) and 49% less likely to receive counseling by a fertility specialist (prevalence ratio: 0.51; 95% confidence interval, 0.28–0.93).

Conclusions:

Women who lived further away from fertility clinics were less likely to receive fertility counseling.

Impact:

Interventions to improve access to fertility counseling should include strategies to alleviate the burden of geographic access.
Tumor-Localized Interleukin-2 and Interleukin-12 Combine with Radiation Therapy to Safely Potentiate Regression of Advanced Malignant Melanoma in Pet Dogs
Authors: Stinson Jordan A. , Barbosa Matheus Moreno P. , Sheen Allison , Momin Noor , Fink Elizabeth , Hampel Jordan , Selting Kim A. , Kamerer Rebecca L. , Bailey Keith L. , Wittrup Karl D. , Fan Timothy M.
Published: July 2024

Purpose:

Cytokines IL2 and IL12 exhibit potent anticancer activity but suffer a narrow therapeutic window due to off-tumor immune cell activation. Engineering cytokines with the ability to bind and associate with tumor collagen after intratumoral injection potentiated response without toxicity in mice and was previously safe in pet dogs with sarcoma. Here, we sought to test the efficacy of this approach in dogs with advanced melanoma.

Patients and Methods:

This study examined 15 client-owned dogs with histologically or cytologically confirmed malignant melanoma that received a single 9-Gy fraction of radiotherapy, followed by six cycles of combined collagen-anchored IL2 and IL12 therapy every 2 weeks. Cytokine dosing followed a 3 + 3 dose escalation design, with the initial cytokine dose chosen from prior evaluation in canine sarcomas. No exclusion criteria for tumor stage or metastatic burden, age, weight, or neuter status were applied for this trial.

Results:

Median survival regardless of the tumor stage or dose level was 256 days, and 10/13 (76.9%) dogs that completed treatment had CT-measured tumor regression at the treated lesion. In dogs with metastatic disease, 8/13 (61.5%) had partial responses across their combined lesions, which is evidence of locoregional response. Profiling by NanoString of treatment-resistant dogs revealed that B2m loss was predictive of poor response to this therapy.

Conclusions:

Collectively, these results confirm the ability of locally administered tumor-anchored cytokines to potentiate responses at regional disease sites when combined with radiation. This evidence supports the clinical translation of this approach and highlights the utility of comparative investigation in canine cancers.
Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Authors: Chang Ju-Fang , Landmann Jack H. , Chang Tien-Ching , Selli Mehmet Emrah , Tenzin Yangdon , Warrington John M. , Ritchey Julie , Hsu Yu-Sung , Slade Michael , Gupta Deepesh Kumar , DiPersio John F. , Holehouse Alex S. , Singh Nathan
Published: July 2024

Chimeric antigen receptor (CAR)–based therapies have pioneered synthetic cellular immunity but remain limited in their long-term efficacy. Emerging data suggest that dysregulated CAR-driven T-cell activation causes T-cell dysfunction and therapeutic failure. To re-engage the precision of the endogenous T-cell response, we designed MHC-independent T-cell receptors (miTCR) by linking antibody variable domains to T-cell receptor constant chains. Using predictive modeling, we observed that this standard “cut and paste” approach to synthetic protein design resulted in myriad biochemical conflicts at the hybrid variable–constant domain interface. Through iterative modeling and sequence modifications, we developed structure-enhanced miTCRs which significantly improved receptor-driven T-cell function across multiple tumor models. We found that 41BB costimulation specifically prolonged miTCR T-cell persistence and enabled improved leukemic control in vivo compared with classic CAR T cells. Collectively, we have identified core features of hybrid receptor structure responsible for regulating function.

Significance: Improving the durability of engineered T-cell immunotherapies is critical to enhancing efficacy. We used a structure-informed design to evolve improved miTCR function across several models. This work underscores the central role of synthetic receptor structure in T-cell function and provides a framework for improved receptor engineering.
Supplementary Figure 9 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Authors: Sigafoos Ashley N. , Tolosa Ezequiel J. , Carr Ryan M. , Fernandez-Barrena Maite G. , Almada Luciana L. , Pease David R. , Hogenson Tara L. , Raja Arul Glancis L. , Mousavi Fatemeh , Sen Sandhya , Vera Renzo E. , Marks David L. , Flores Luis F. , LaRue-Nolan Kayla C. , Wu Chen , Bamlet William R. , Vrabel Anne M. , Sicotte Hugues , Schenk Erin L. , Smyrk Thomas C. , Zhang Lizhi , Rabe Kari G. , Oberg Ann L. , Zaphiropoulos Peter G. , Chevet Eric , Graham Rondell P. , Hagen Catherine E. , di Magliano Marina P. , Elsawa Sherine F. , Pin Christopher L. , Mao Junhao , McWilliams Robert R. , Fernandez-Zapico Martin E.
Published: July 2024

Supplementary Figure 9 shows that Gli2 does not change H3K27Ac enrichment at Ccnd1 promoter downstream of oncogenic KRAS.
Supplementary Figure 8 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Authors: Sigafoos Ashley N. , Tolosa Ezequiel J. , Carr Ryan M. , Fernandez-Barrena Maite G. , Almada Luciana L. , Pease David R. , Hogenson Tara L. , Raja Arul Glancis L. , Mousavi Fatemeh , Sen Sandhya , Vera Renzo E. , Marks David L. , Flores Luis F. , LaRue-Nolan Kayla C. , Wu Chen , Bamlet William R. , Vrabel Anne M. , Sicotte Hugues , Schenk Erin L. , Smyrk Thomas C. , Zhang Lizhi , Rabe Kari G. , Oberg Ann L. , Zaphiropoulos Peter G. , Chevet Eric , Graham Rondell P. , Hagen Catherine E. , di Magliano Marina P. , Elsawa Sherine F. , Pin Christopher L. , Mao Junhao , McWilliams Robert R. , Fernandez-Zapico Martin E.
Published: July 2024

Supplementary Figure 8 shows the expression of GLI target genes in all experimental groups.
Supplementary Figure 5 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Authors: Sigafoos Ashley N. , Tolosa Ezequiel J. , Carr Ryan M. , Fernandez-Barrena Maite G. , Almada Luciana L. , Pease David R. , Hogenson Tara L. , Raja Arul Glancis L. , Mousavi Fatemeh , Sen Sandhya , Vera Renzo E. , Marks David L. , Flores Luis F. , LaRue-Nolan Kayla C. , Wu Chen , Bamlet William R. , Vrabel Anne M. , Sicotte Hugues , Schenk Erin L. , Smyrk Thomas C. , Zhang Lizhi , Rabe Kari G. , Oberg Ann L. , Zaphiropoulos Peter G. , Chevet Eric , Graham Rondell P. , Hagen Catherine E. , di Magliano Marina P. , Elsawa Sherine F. , Pin Christopher L. , Mao Junhao , McWilliams Robert R. , Fernandez-Zapico Martin E.
Published: July 2024

Supplementary Figure 5 shows the IHC results looking at CD4 and CD8 expression in KC and KCRG mice. The results show no difference in the immune landscape between these mouse models.
Supplementary Figure 4 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Authors: Sigafoos Ashley N. , Tolosa Ezequiel J. , Carr Ryan M. , Fernandez-Barrena Maite G. , Almada Luciana L. , Pease David R. , Hogenson Tara L. , Raja Arul Glancis L. , Mousavi Fatemeh , Sen Sandhya , Vera Renzo E. , Marks David L. , Flores Luis F. , LaRue-Nolan Kayla C. , Wu Chen , Bamlet William R. , Vrabel Anne M. , Sicotte Hugues , Schenk Erin L. , Smyrk Thomas C. , Zhang Lizhi , Rabe Kari G. , Oberg Ann L. , Zaphiropoulos Peter G. , Chevet Eric , Graham Rondell P. , Hagen Catherine E. , di Magliano Marina P. , Elsawa Sherine F. , Pin Christopher L. , Mao Junhao , McWilliams Robert R. , Fernandez-Zapico Martin E.
Published: July 2024

Supplementary Figure 4 shows the validation of Kras signaling activation and chronic pancreatitis phenotypic examples in KC and KCRG mice.
Supplementary Figure 3 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Authors: Sigafoos Ashley N. , Tolosa Ezequiel J. , Carr Ryan M. , Fernandez-Barrena Maite G. , Almada Luciana L. , Pease David R. , Hogenson Tara L. , Raja Arul Glancis L. , Mousavi Fatemeh , Sen Sandhya , Vera Renzo E. , Marks David L. , Flores Luis F. , LaRue-Nolan Kayla C. , Wu Chen , Bamlet William R. , Vrabel Anne M. , Sicotte Hugues , Schenk Erin L. , Smyrk Thomas C. , Zhang Lizhi , Rabe Kari G. , Oberg Ann L. , Zaphiropoulos Peter G. , Chevet Eric , Graham Rondell P. , Hagen Catherine E. , di Magliano Marina P. , Elsawa Sherine F. , Pin Christopher L. , Mao Junhao , McWilliams Robert R. , Fernandez-Zapico Martin E.
Published: July 2024

Supplementary Figure 3 shows Gli2 expression in CRG and KCRG mice and Gli luciferase activity in ΔNGli2-transfected cells.
Figure 4 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Authors: Sigafoos Ashley N. , Tolosa Ezequiel J. , Carr Ryan M. , Fernandez-Barrena Maite G. , Almada Luciana L. , Pease David R. , Hogenson Tara L. , Raja Arul Glancis L. , Mousavi Fatemeh , Sen Sandhya , Vera Renzo E. , Marks David L. , Flores Luis F. , LaRue-Nolan Kayla C. , Wu Chen , Bamlet William R. , Vrabel Anne M. , Sicotte Hugues , Schenk Erin L. , Smyrk Thomas C. , Zhang Lizhi , Rabe Kari G. , Oberg Ann L. , Zaphiropoulos Peter G. , Chevet Eric , Graham Rondell P. , Hagen Catherine E. , di Magliano Marina P. , Elsawa Sherine F. , Pin Christopher L. , Mao Junhao , McWilliams Robert R. , Fernandez-Zapico Martin E.
Published: July 2024

Gli2 drives H3K4me3 enrichment at Ccnd1 promoter in mutant KRAS cells. A, Heatmaps representing global levels of H3K4me3 enrichment in 1012U −Dox and 1012U +Dox cells. B, MA-plot highlighting differentially enriched H3K4me3 peaks in the 1012U +Dox cells compared with the 1012U −Dox cells. C, Profile plot of H3K4me3 at the transcriptional start site + or − 5 kb comparing 1012U +Dox cells with the 1012U −Dox cells. D, ChIP-seq tracks showing enrichment of H3K4me3 in 1012U –Dox and +Dox cells for GLI target gene Ccnd1. E, Western blot (left) and protein quantification (right) representing expression of H3K4me3 in 1012U cells +Dox. Total H3 is used as the loading control. F, H3K4me3 enrichment at Ccdn1 promoter in 1012U +Dox cells postknockdown of Gli2.
Figure 2 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Authors: Sigafoos Ashley N. , Tolosa Ezequiel J. , Carr Ryan M. , Fernandez-Barrena Maite G. , Almada Luciana L. , Pease David R. , Hogenson Tara L. , Raja Arul Glancis L. , Mousavi Fatemeh , Sen Sandhya , Vera Renzo E. , Marks David L. , Flores Luis F. , LaRue-Nolan Kayla C. , Wu Chen , Bamlet William R. , Vrabel Anne M. , Sicotte Hugues , Schenk Erin L. , Smyrk Thomas C. , Zhang Lizhi , Rabe Kari G. , Oberg Ann L. , Zaphiropoulos Peter G. , Chevet Eric , Graham Rondell P. , Hagen Catherine E. , di Magliano Marina P. , Elsawa Sherine F. , Pin Christopher L. , Mao Junhao , McWilliams Robert R. , Fernandez-Zapico Martin E.
Published: July 2024

KCRG mice show increased tumor proliferation. A, Representative IHC images (left) and quantification (right) for the cell proliferation marker Ki67 (brown signal) in Cre (n = 3), CRG (n = 3), KC (n = 4), and KCRG (n = 4) mice. Scale bar, 200 μm. B, Masson trichrome staining immunocytochemistry representative images (left) and quantification (right) for collagen (blue signal) in Cre (n = 3) and CRG (n = 3) mice and KC (n = 4) and KCRG (n = 4) mice. Scale bar, 200 μm. C, Representative IHC images (left) and quantification (right) for macrophage marker F4/80 (brown signal) in Cre (n = 3), CRG (n = 3), KC (n = 4), and KCRG (n = 4) mice. Scale bar, 200 μm.
A arte como um instrumento para combater a psicofobia: relato de experiência sobre a oficina “Jornada da luta Antimanicomial”
Authors: Menezes Yasmin Cavalcanti de , Silva Márcia dos Santos
Published: July 2024

The Brazilian anti-asylum movement has been intertwined with art over the decades, influencing the transformation of psychiatric care and psychosocial imaginary. However, the replacement of asylums with freedom-oriented care is jeopardized if there are no professionals who are part of and advocate for humanitarian mental health care. Therefore, it is extremely necessary to incorporate these themes into the education of future doctors. Thus, the report describes the realization of the “Anti-Asylum Movement Journey” at a private medical school, a workshop which took place on May 18th, National Anti-Asylum Day. It included a poetic soirée, an exploration of the history of art related to mental health, and creative proposals to engage users of the psychosocial care network. The event aims to recall the history of the anti-asylum struggle and reaffirm its necessity, utilizing art as the primary tool to explore ethics and policies that ensure visibility, inclusion, and assistance for mental health patients in their environment. As a result, participants learned the importance and methods of using art in healthcare, preparing them for a more sensitive, critical and politically engaged medical practice. The event also destigmatized and humanized the perception of mental illness in a strictly academic environment.
Serviço de Referência Virtual: uma análise estratégica por meio da aplicação da matriz SWOT
Authors: Damian Ieda Pelogia Martins , Silva Marcia Regina
Published: July 2024

O Serviço de Referência Virtual (SRV) diz respeito às formas de prestação de serviço de referência e informação por meioda utilização de tecnologia da informação, com o intuito de oferecer uma série de benefícios aos seus usuários. Como ainda se trata de uma tecnologia recente, existem muitos recursos que podem ser explorados em benefício de todos os envolvidos, como as bibliotecas universitárias e usuários deste serviço. Para que estes benefícios possam ser usufruídos, este estudo se propõe a aplicar a metodologia de análise estratégica SWOT em um site de uma biblioteca universitária, com a intenção de identificar os principais pontos fortes, pontos fracos, ameaças e oportunidades do serviço de referência virtual. Foi possível verificar que o SRV é uma oportunidade para aumentar a satisfação do usuário e, também, a interação das bibliotecas universitárias com seus usuários.
Conteúdo informacional gerado nas redes sociais: o universo dos parques de diversão
Authors: Presser Nadi Helena , Menezes Phelipe Rafael Alves de
Published: July 2024

Pesquisa exploratória e descritiva, examinou os mecanismos de interatividade fornecidos pelas redes sociais e identificou os conteúdos informacionais gerado pelos internautas. A unidade de análise se constituiu de três sites de relacionamento – Facebook, Twitter e Instagram, dos quais foram examinados os perfis de quatro parques de diversão brasileiros – Beto Carrero, Beach Park, Hot Park e Thermas dos Laranjais. Os resultados sugeriram a criação de três categorias de análise: identificação do público dos parques de diversão, a interatividade informacional nas redes sociais e, por último, uma descrição das manifestações do público sobre os parques de diversão. Em relação à interatividade, o estudo constatou que a produção, disseminação e uso de informação configura um contexto social, no qual cada pessoa é um potencial produtor e usuário de informações. Os horários e períodos de captura e registro de fotos e outras publicações refletem a experiência turística ocorrendo durante a visita ao parque e capturada em tempo real. O conteúdo informativo desvela que os parques, em geral, possuem um bom nível de menções positivas.
Eisenstein's Paris Diary: An Introduction
Authors: Rebecchi Marie , Vogman Elena
Published: July 2024

This introductory text unfolds and interprets some key elements from Eisenstein's Paris Diary, contextualizing his encounters and providing further details for understanding the filmmaker's fragmentary and sometimes cryptic diary as an autotheoretical practice. Written from the perspective of one immersed in the artistic and intellectual Paris of 1929 and 1930—as evidenced by Eisenstein's encounters with Colette, Tristan Tzara, Gertrude Stein, André Malraux, Filippo T. Marinetti, Abel Gance, Jean Cocteau, Louis Aragon, Paul Éluard, Kiki de Montparnasse, Darius Milhaud, and others—the diary highlights the importance of his exchange with the avant-garde micro-cinematographer Jean Painlevé, as well as his theoretical and political kinship with the heterodox Surrealist group around the journal Documents. The introduction investigates the way in which Eisenstein's Paris Diary both documents daily events and serves as an ephemeral depository for the author's theoretical ideas on filmmaking and montage, seeing these in terms of such late works as Method, Montage, and Disney.
The Reflex Republic: Physiologies of Art in the Early Soviet Union
Authors: Vollgraff Matthew
Published: July 2024

In the immediate wake of the Russian Revolution, Pavlovian reflex conditioning was rapidly elevated to a universal materialist model for understanding and manipulating human behavior, including the production and reception of art. This article explores the little-known history of the “reflexology of art,” a movement that applied physiological concepts and techniques to drama, cinema, and literature with the aim of molding atomized individual subjectivities into a corporeal collective. This movement—which involved figures like Vsevolod Meyerhold, Sergei Eisenstein, and Sergei Tret'iakov—formed part of the highly politicized dialogue between science, politics and the arts in the 1920s. By reconstructing the critical discourse around the reflexology of art within the cultural production of the early Soviet state, the article sheds light on the contradictory impulses animating an avant-garde caught between deterministic visions of social engineering and the subversive passions of revolution.
New discoveries for the genus Miconia (Melastomataceae): a new species for the brazilian Atlantic Forest
Authors: AUGUSTIN ANA FLÁVIA , NERY EDUARDO KOERICH , CADDAH MAYARA KRASINSKI
Published: July 2024

We describe Miconia leonorae, a new species exclusive to eastern Brazil, mainly from the evergreen forests of the Atlantic Forest Domain. This species can be distinguished from a wider circumscription of Miconia lepidota. It is classified as Miconia supersect. Discolores sect. Multispicatae and can be distinguished from similar species by features such as an acute leaf base and apex, light brown to gray abaxial leaf blade indumentum with lepidote-stellate trichomes (becoming glabrescent on mature leaves), cylindrical, scorpioid inflorescences with basal branches only bifid or trifid, and an androecium with isomorphic stamens.
A new species of short unpatterned moray eel (Anguilliformes: Muraenidae) from the northwestern Indian Ocean, including the Socotra Archipelago, with a redescription of Gymnothorax pseudoherrei Böhlke
Authors: SMITH DAVID G. , BOGORODSKY SERGEY V. , DANDAR JAMES , ZAJONZ UWE
Published: July 2024

A new species of short brown unpatterned moray eel, Gymnothorax. arabicus sp. nov., is described based on specimens collected from the northwestern Indian Ocean, including the Red Sea, Socotra Archipelago, and Arabian Gulf. The new species is characterized by having the dorsal-fin origin before the gill opening, two branchial pores, one or two median intermaxillary teeth, biserial maxillary teeth, total vertebrae 114–120, and a plain brown head and body with dark yellow color at the tip of the tail. The new species formerly has been confused with G. pseudoherrei but is distinguished from it by having slightly more total vertebrae (114–120 vs. 111–116), dark stripes on the throat and side of the head, and a larger maximum size (ca. 300 mm TL vs. ca. 200 mm TL). Gymnothorax pseudoherrei, currently known from the Maldives and Sri Lanka to the Philippines, Solomon Islands, and northern Australia, is redescribed in the present study. A comparison of the COI mtDNA in BOLD, in association with published phylogenetic evidence, confirms that G. arabicus and G. pseudoherrei form two separate genetic lineages, corroborating the morphological data.
Natural variation of domestication-related genes contributed to latitudinal expansion and adaptation in soybean
Authors: Li Jing , Li Yecheng , Agyenim-Boateng Kwadwo Gyapong , Shaibu Abdulwahab Saliu , Liu Yitian , Feng Yue , Qi Jie , Li Bin , Zhang Shengrui , Sun Junming
Published: July 2024

Soybean is a major source of protein and edible oil worldwide. Originating from the Huang-Huai-Hai region, which has a temperate climate, soybean has adapted to a wide latitudinal gradient across China. However, the genetic mechanisms responsible for the widespread latitudinal adaptation in soybean, as well as the genetic basis, adaptive differentiation, and evolutionary implications of theses natural alleles, are currently lacking in comprehensive understanding. In this study, we examined the genetic variations of fourteen major gene loci controlling flowering and maturity in 103 wild species, 1048 landraces, and 1747 cultivated species. We found that E1, E3, FT2a, J, Tof11, Tof16, and Tof18 were favoured during soybean improvement and selection, which explained 75.5% of the flowering time phenotypic variation. These genetic variation was significantly associated with differences in latitude via the LFMM algorithm. Haplotype network and geographic distribution analysis suggested that gene combinations were associated with flowering time diversity contributed to the expansion of soybean, with more HapA clustering together when soybean moved to latitudes beyond 35°N. The geographical evolution model was developed to accurately predict the suitable planting zone for soybean varieties. Collectively, by integrating knowledge from genomics and haplotype classification, it was revealed that distinct gene combinations improve the adaptation of cultivated soybeans to different latitudes. This study provides insight into the genetic basis underlying the environmental adaptation of soybean accessions, which could contribute to a better understanding of the domestication history of soybean and facilitate soybean climate-smart molecular breeding for various environments.
Assessment of COVID-19 risk factors of early and long-term mortality with prediction models of clinical and laboratory variables
Authors: Lipski Dawid , Radziemski Artur , Wasiliew Stanisław , Wyrwa Michał , Szczepaniak-Chicheł Ludwina , Stryczyński Łukasz , Olasińska-Wiśniewska Anna , Urbanowicz Tomasz , Perek Bartłomiej , Tykarski Andrzej , Komosa Anna
Published: July 2024

Background

Coronavirus disease (COVID-19) may lead to serious complications and increased mortality. The outcomes of patients who survive the early disease period are burdened with persistent long-term symptoms and increased long-term morbidity and mortality. The aim of our study was to determine which baseline parameters may provide the best prediction of early and long-term outcomes.

Methods

The study group comprised 141 patients hospitalized for COVID-19. Demographic data, clinical data and laboratory parameters were collected. The main study endpoints were defined as in-hospital mortality and 1-year mortality. The associations between the baseline data and the study endpoints were evaluated. Prediction models were created.

Results

The in-hospital mortality rate was 20.5% (n = 29). Compared with survivors, nonsurvivors were significantly older (p = 0.001) and presented comorbidities, including diabetes (0.027) and atrial fibrillation (p = 0.006). Assessment of baseline laboratory markers and time to early death revealed negative correlations between time to early death and higher IL-6 levels (p = 0.032; Spearman rho − 0.398) and lower lymphocyte counts (p = 0.018; Pearson r -0.438). The one-year mortality rate was 35.5% (n = 50). The 1-year nonsurvivor subgroup was older (p < 0.001) and had more patients with arterial hypertension (p = 0.009), diabetes (p = 0.023), atrial fibrillation (p = 0.046) and active malignancy (p = 0.024) than did the survivor subgroup. The model composed of diabetes and atrial fibrillation and IL-6 with lymphocyte count revealed the highest value for 1-year mortality risk prediction.

Conclusions

Diabetes and atrial fibrillation, as clinical factors, and LDH, IL-6 and lymphocyte count, as laboratory determinants, are the best predictors of COVID-19 mortality risk.