bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Insights in Atrial Fibrillation Pathogenesis and Therapeutics: A Narrative Review
Authors: Papathanasiou Konstantinos A. , Giotaki Sotiria G. , Vrachatis Dimitrios A. , Siasos Gerasimos , Lambadiari Vaia , Iliodromitis Konstantinos E. , Kossyvakis Charalampos , Kaoukis Andreas , Raisakis Konstantinos , Deftereos Gerasimos , Papaioannou Theodore G. , Giannopoulos Georgios , Avramides Dimitrios , Deftereos Spyridon G.
Published: September 2021

The prevalence of atrial fibrillation (AF) is bound to increase globally in the following years, affecting the quality of life of millions of people, increasing mortality and morbidity, and beleaguering health care systems. Increasingly effective therapeutic options against AF are the constantly evolving electroanatomic substrate mapping systems of the left atrium (LA) and ablation catheter technologies. Yet, a prerequisite for better long-term success rates is the understanding of AF pathogenesis and maintenance. LA electrical and anatomical remodeling remains in the epicenter of current research for novel diagnostic and treatment modalities. On a molecular level, electrical remodeling lies on impaired calcium handling, enhanced inwardly rectifying potassium currents, and gap junction perturbations. In addition, a wide array of profibrotic stimuli activates fibroblast to an increased extracellular matrix turnover via various intermediaries. Concomitant dysregulation of the autonomic nervous system and the humoral function of increased epicardial adipose tissue (EAT) are established mediators in the pathophysiology of AF. Local atrial lymphomononuclear cells infiltrate and increased inflammasome activity accelerate and perpetuate arrhythmia substrate. Finally, impaired intracellular protein metabolism, excessive oxidative stress, and mitochondrial dysfunction deplete atrial cardiomyocyte ATP and promote arrhythmogenesis. These overlapping cellular and molecular alterations hinder us from distinguishing the cause from the effect in AF pathogenesis. Yet, a plethora of therapeutic modalities target these molecular perturbations and hold promise in combating the AF burden. Namely, atrial selective ion channel inhibitors, AF gene therapy, anti-fibrotic agents, AF drug repurposing, immunomodulators, and indirect cardiac neuromodulation are discussed here.
The Role of EUS and EUS-FNA in Differentiating Benign and Malignant Gallbladder Lesions
Authors: Hijioka Susumu , Nagashio Yoshikuni , Ohba Akihiro , Maruki Yuta , Okusaka Takuji
Published: September 2021

Endoscopic ultrasonography (EUS) has greater spatial resolution than other diagnostic imaging modalities. In addition, if gallbladder lesions are found and gallbladder cancer is suspected, EUS is an indispensable modality, enabling detailed tests for invasion depth evaluation using the Doppler mode and ultrasound agents. Furthermore, for gallbladder lesions, EUS fine-needle aspiration (EUS-FNA) can be used to differentiate benign and malignant forms of conditions, such as xanthogranulomatous cholecystitis, and collect evidence before chemotherapy. EUS-FNA is also useful for highly precise and specific diagnoses. However, the prevention of bile leakage, an accidental symptom, is highly important. Advancements in next-generation sequencing (NGS) technologies facilitate the application of multiple parallel sequencing to EUS-FNA samples. Several biomarkers are expected to stratify treatment for gallbladder cancer; however, NGS can unveil potential predictive genomic biomarkers for the treatment response. It is believed that NGS may be feasible with samples obtained using EUS-FNA, further increasing the demand for EUS-FNA.
Comparison between an Emerging Point-of-Care Tool for TSH Evaluation and a Centralized Laboratory-Based Method in a Cohort of Patients from Southern Italy
Authors: Di Cerbo Alfredo , Quagliano Nazario , Napolitano Antonella , Pezzuto Federica , Iannitti Tommaso , Di Cerbo Alessandro
Published: September 2021

Endocrine and metabolic disorders are a common condition in Europe and worldwide, and, among these, thyroid dysfunction still remains a problem. The measurement of thyroid stimulating hormone (TSH) levels represents the first-line assay for the assessment of thyroid function. In the present study, we compared serum concentrations of TSH, measured using a commercially available point-of-care test (POCT) method (FastPack® IP) and an established “conventional” laboratory-based method (Beckmann Access 2) in a cohort of patients from Foggia in Southern Italy. A strong correlation (r = 0.994) was found between both methods and was also confirmed by receiver operating characteristic (ROC) curve analysis (0.82). The within-run coefficient of variation (CV) using FastPack® ranged from 4.03% and 8.57% at the TSH concentrations of 39.49 and 0.70 mIU/L, respectively. The between-run CV was 10.34% and 6.33% at the TSH concentrations of 0.87 and 26.55 mIU/L, respectively. The ratios of within- to between-assay CV were 0.83 and 1.06 at the TSH levels of 0.70 and 52.59 mIU/mL, respectively. In this study, we showed that serum TSH levels can be measured in a few minutes and at low-cost in terms of materials and equipment required. We observed that this approach is user-friendly, accurate, reproducible, and suitable for use in the clinic, while also meeting the criteria for effectiveness, impact, efficiency, and sustainability.
A First Ever Look into Greece’s Vast Educational Data: Interesting Findings and Policy Implications
Authors: Papadogiannis Ilias , Wallace Manolis , Poulopoulos Vassilis , Karountzou Georgia , Ekonomopoulos Dimitris
Published: September 2021

Intro: In this survey the academic performance of primary and secondary school students in Greece, for three consecutive school years, was examined. The data concerned all Greek students of the last two grades of elementary school and the three grades of junior high school. Method: Unsupervised learning methods such as an X-means algorithm in combination with descriptive and inductive statistical methods were used, in order to examine students’ performance levels. The longitudinal stability of academic performance levels and the influence of demographic characteristics such as the region, gender and guardians’ profession were also examined. Results: The existence of four levels of academic performance and longitudinal stability of frequencies per performance level was confirmed. There was also statistically significant differentiation based on the profession of guardian, gender, and area of residence. Discussion: The results demonstrated specific challenges that the educational policy of the country has to address. The stability of the percentages of students in the four groups of academic performance that emerged over time, shows corresponding stability in the factors that affect academic performance. A gradual reduction in the performance of students in high School was found, as the level of difficulty of the courses increases from class to class. Some demographic characteristics of students are not independent of their performance. However, due to the compliance with the general regulation of personal data, there was no access to additional features that may be related to performance, such as nationality and exact place of residence.
Safer Systems: People Training or System Tuning?
Authors: Hollnagel Erik
Published: September 2021

Safety is usually seen as a problem when it is absent rather than when it is present, where accidents, incidents, and the like represent a lack of safety rather than the presence of safety. To explain this lack of safety, one or more causes must be found. In the management of industrial safety, the human factor has traditionally been seen as a weak element; human error is often offered as the first, and sometimes the only cause of lack of safety and human factors have since the early days offered three principal solutions, namely training, design, and automation. Of these, training has considerable face value as an effective means to improve human performance. The drawback of safety training, however, is that it focuses on a single system component, the human, instead of on the system as a whole. Safety training further takes for granted that humans are a liability and focuses on overcoming the weakness of this specific component through simplistic models of what determines human performance. But humans may also be seen as an asset which changes the focus to strengthening how a complex socio-technical system functions. A socio-technical system comprises multiple functions that must be finely tuned in order to ensure expected and acceptable performance. Since systems cannot be made safer without developing effective ways of managing the conditions in which people work, system tuning offers an alternative solution to an old problem.
Light in Electrochemistry
Authors: Abe Hiroya , Iwama Tomoki , Guo Yuanyuan
Published: September 2021

Electrochemistry represents an important analytical technique used to acquire and assess chemical information in detail, which can aid fundamental investigations in various fields, such as biological studies. For example, electrochemistry can be used as simple and cost-effective means for bio-marker tracing in applications, such as health monitoring and food security screening. In combination with light, powerful spatially-resolved applications in both the investigation and manipulation of biochemical reactions begin to unfold. In this article, we focus primarily on light-addressable electrochemistry based on semiconductor materials and light-readable electrochemistry enabled by electrochemiluminescence (ECL). In addition, the emergence of multiplexed and imaging applications will also be introduced.
Waveform Design for 5G and beyond Systems
Authors: Choi Kwonhue
Published: September 2021

Currently, 5G communication systems are being commercially deployed in many countries [...]
Reporting Thyroid Cytology in a Globalized World
Authors: Pizzimenti Cristina , Mazzeo Francesca , Militi Gaetano Basilio , Tuccari Giovanni , Ieni Antonio , Rossi Esther Diana , Fadda Guido
Published: September 2021

The Italian SIAPEC-AIT 2014 classification, the 2017 Bethesda System for Reporting Thyroid Cytology (TBSRTC), the 2016 UK Royal College of Pathologists (RCPath) thyroid reporting system, and the 2019 Japanese reporting system for thyroid aspiration cytology (JRSTAC2019) represent the most widely used reporting systems among clinicians and pathologists for the purpose of cytologically diagnosing, estimating the potential risk of malignancy (ROM), and defining the most appropriate treatment for a patient with a thyroid nodule. Although all the systems use overlapping diagnostic categories and morphologic criteria, they differ on the basis of the criteria for inclusion in the cytologic categories, which may, in turn, affect the ROM of a given category and the clinical management of the patient, particularly with regard to the “indeterminate” categories. The aim of this review is to analyze the main differences that emerge between the systems and to propose possible solutions for a comprehensive reporting system that integrates and harmonizes all the criteria of the Italian classification and the Bethesda system, also taking into account the impact that the new tumor entity NIFTP (non-invasive follicular tumor with papillary-like nuclear features) that has, in many instances, replaced the non-invasive form of the follicular variant of papillary carcinoma, has had on the modification of malignancy risks.
Insight into the Effects of Solvent Treatment of Natural Fibers Prior to Structural Composite Casting: Chemical, Physical and Mechanical Evaluation
Authors: Abbass Ali , Paiva Maria C. , Oliveira Daniel V. , Lourenço Paulo B. , Fangueiro Raul
Published: September 2021

This paper presents an optimized washing protocol for as-received natural fibers, prior to large-scale composite manufacturing, for the structural strengthening of historic masonry. The aim was to achieve a simple protocol for standard cleaning of fiber surfaces from low molecular weight constituents that may be detrimental towards interfacial strength without damaging the fibers. The proposed procedure employs the application of the solvent sequence: ethanol, acetone, hexane, with optimized incubation times and stirring conditions. Additionally, this procedure may change the surface of the fiber, thereby enhancing the durability of the fiber-matrix interface. The washing protocol resulted in an increase of tensile strength by 56%, 52% and 22% for flax, hemp and sisal fibers, respectively, as compared to the corresponding non-washed fibers, without loss of elongation. The static contact angle measurements confirmed exposure of a higher fraction of the hydrophilic crystalline cellulose, with a higher wettability observed after washing protocols.
Sustainably Processed Waste Wool Fiber-Reinforced Biocomposites for Agriculture and Packaging Applications
Authors: Bhavsar Parag , Balan Tudor , Dalla Fontana Giulia , Zoccola Marina , Patrucco Alessia , Tonin Claudio
Published: September 2021

In the EU, sheep bred for dairy and meat purposes are of low quality, their economic value is not even enough to cover shearing costs, and their wool is generally seen as a useless by-product of sheep farming, resulting in large illegal disposal or landfilling. In order to minimize environmental and health-related problems considering elemental compositions of discarded materials such as waste wool, there is a need to recycle and reuse waste materials to develop sustainable innovative technologies and transformation processes to achieve sustainable manufacturing. This study aims to examine the application of waste wool in biocomposite production with the help of a sustainable hydrolysis process without any chemicals and binding material. The impact of superheated water hydrolysis and mixing hydrolyzed wool fibers with kraft pulp on the performance of biocomposite was investigated and characterized using SEM, FTIR, tensile strength, DSC, TGA, and soil burial testing in comparison with 100% kraft pulp biocomposite. The superheated water hydrolysis process increases the hydrophilicity and homogeneity and contributes to increasing the speed of biodegradation. The biocomposite is entirely self-supporting, provides primary nutrients for soil nourishment, and is observed to be completely biodegradable when buried in the soil within 90 days. Among temperatures tested for superheated water hydrolysis of raw wool, 150 °C seems to be the most appropriate for the biocomposite preparation regarding physicochemical properties of wool and suitability for wool mixing with cellulose. The combination of a sustainable hydrolysis process and the use of waste wool in manufacturing an eco-friendly, biodegradable paper/biocomposite will open new potential opportunities for the utilization of waste wool in agricultural and packaging applications and minimize environmental impact.
How Vulnerable Are American States to Wildfires? A Livelihood Vulnerability Assessment
Authors: Baijnath-Rodino Janine A. , Kumar Mukesh , Rivera Margarita , Tran Khoa D. , Banerjee Tirtha
Published: September 2021

Quantifying livelihood vulnerability to wildland fires in the United States is challenging because of the need to systematically integrate multidimensional variables into its analysis. We aim to measure wildfire threats amongst humans and their physical and social environment by developing a framework to calculate the livelihood vulnerability index (LVI) for the top 14 American states most recently exposed to wildfires. The LVI is computed by assessing each state’s contributing factors (exposure, sensitivity, and adaptive capacity) to wildfire events. These contributing factors are determined through a set of indicator variables that are categorized into corresponding groups to produce an LVI framework. The framework is validated by performing a principal component analysis (PCA), ensuring that each selected indicator variable corresponds to the correct contributing factor. Our results indicate that Arizona and New Mexico experience the greatest livelihood vulnerability. In contrast, California, Florida, and Texas experience the least livelihood vulnerability. While California has one of the highest exposures and sensitivity to wildfires, results indicate that it has a relatively high adaptive capacity, in comparison to the other states, suggesting it has measures in place to withstand these vulnerabilities. These results are critical to wildfire managers, government, policymakers, and research scientists for identifying and providing better resiliency and adaptation measures to support states that are most vulnerable to wildfires.
Resistant Starches and Non-Communicable Disease: A Focus on Mediterranean Diet
Authors: Cione Erika , Fazio Alessia , Curcio Rosita , Tucci Paola , Lauria Graziantonio , Cappello Anna Rita , Dolce Vincenza
Published: September 2021

Resistant starch (RS) is the starch fraction that eludes digestion in the small intestine. RS is classified into five subtypes (RS1–RS5), some of which occur naturally in plant-derived foods, whereas the others may be produced by several processing conditions. The different RS subtypes are widely found in processed foods, but their physiological effects depend on their structural characteristics. In the present study, foods, nutrition and biochemistry are summarized in order to assess the type and content of RS in foods belonging to the Mediterranean Diet (MeD). Then, the benefits of RS consumption on health are discussed, focusing on their capability to enhance glycemic control. RS enters the large bowel intestine, where it is fermented by the microbiome leading to the synthesis of short-chain fatty acids as major end products, which in turn have systemic health effects besides the in situ one. It is hoped that this review will help to understand the pros of RS consumption as an ingredient of MeD food. Consequently, new future research directions could be explored for developing advanced dietary strategies to prevent non-communicable diseases, including colon cancer.
Contamination of Fusarium proliferatum and Aspergillus flavus in the Rice Chain Linked to Crop Seasons, Cultivation Regions, and Traditional Agricultural Practices in Mekong Delta, Vietnam
Authors: Phan Lien Thi Kim , Tran Trang Minh , Audenaert Kris , Jacxsens Liesbeth , Eeckhout Mia
Published: September 2021

This study evaluates the influence of crop seasons, cultivation regions, and traditional agricultural practices on the occurrence of F. proliferatum and A. flavus in the rice chain in the Mekong Delta, Vietnam. A survey on pre- and post-harvest practices was performed from 2017 to 2019 in parallel with sampling. Results showed that F. proliferatum (36.3%) and A. flavus (10%) were predominantly present throughout the rice chain. These fungi frequently occurred in winter–spring and autumn–winter crops in Can Tho paddy. Especially, F. proliferatum appeared both on the field and during transportation (50–100%), while A. flavus presented at all stages (10–33%). The occurrence of F. proliferatum reduced 70–27% after drying, depended on the seasons, compared to field and transportation stages and could not be detected anymore at further stages. Applying poor pre-harvest agricultural practices such as the use of certain varieties (Jasmine, DT8 varieties), combination of fertilizers (organic–inorganic), fields with crop debris, unhygienic boats, and delayed drying time of 8–12 h or 12–28 h resulted in an increase in fungal contamination on paddy. This study provides a detailed description of fungi contamination in crop seasons, cultivation regions, and agricultural practices, which may help in understanding the fungal dynamic and allow identification of good agricultural practices to mitigate the fungal contamination and potential mycotoxin production.
Zipoy-Voorhees Gravitational Object as a Source of High-Energy Relativistic Particles
Authors: Turimov Bobur , Ahmedov Bobomurat
Published: September 2021

The Zipoy-Voorhees solution is known as the γ-metric and/or q-metric being static and axisymmetric vacuum solution of Einstein field equations which becomes strong curvature naked singularity. The metric is characterized by two parameters, namely, the mass M and the dimensionless deformation parameter γ. It is shown that the velocity of test particle orbiting around the central γ-object can reach the speed of light, consequently, the total energy of the particle will be very high for a specific value the deformation parameter of the spacetime. It is also shown that causality problem arises in the interior region of the physical singularity for the specific value of the deformation parameter when test particles can move with superluminal velocity being greater than the speed of light that might be an additional tool for explaining the existence of tachyons for γ>1/2 which are invisible for an observer.
Retrofitting Historic Walls: Feasibility of Thermal Insulation and Suitability of Thermal Mortars
Authors: Posani Magda , Veiga Rosário , de Freitas Vasco Peixoto
Published: September 2021

The European Union is pursuing an ambitious policy on climate action, urgently calling for an acceleration in the transition toward net-zero emissions by 2050. In this context, retrofitting historic constructions can play a key role in reducing European energy consumption and consequent emissions. What is more, beyond the opportunity for tackling climate change, thermal retrofits can improve indoor comfort while lowering operational costs, factors that are fundamental to ensure the continued use of historic constructions over time, and with that, improving their preservation and durability. The suitability of thermal insulation for this scope is still a debated topic. Thus, this study aims at contributing to the discussion by providing an overview on the feasibility of adopting thermal insulation for retrofitting external walls of historic buildings while preserving their significance and unique identities. Finally, the advantages of adopting thermal mortars rather than more traditional insulation solutions are outlined, and their potential efficacy is discussed.
Thromboembolism and Bleeding in COVID-19
Authors: Durak Koray
Published: September 2021

Coronavirus disease 2019 (COVID-19) is characterized by a coagulation dysfunction which has different underlying mechanisms and factors. Patients with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection have an increased risk for thromboembolic and bleeding complications. Incidences are high, and mainly consist of venous thromboembolism (VTE), which significantly contributes to morbidity and mortality in affected patients. Thromboprophylaxis is recommended in all hospitalized COVID-19 patients. Therapeutic doses of antithrombotic agents are only beneficial in noncritically ill patients, and usual care thromboprophylaxis is sufficient in critically ill patients at the ICU. Regarding screening for VTE, high quality evidence is warranted to investigate the significance of asymptomatic DVT in the ICU setting and its influence on PE and mortality.
Single-Incision versus Multiport Robotic Myomectomy: A Propensity Score Matched Analysis of Surgical Outcomes and Surgical Tips
Authors: Lee Sa-Ra , Kim Ju-Hee , Lee Young-Jae , Lee Shin-Wha , Park Jeong-Yeol , Suh Dae-Shik , Kim Dae-Yeon , Kim Sung-Hoon , Kim Yong-Man , Kim Young-Tak
Published: September 2021

We aimed to compare the perioperative outcomes of single-incision robotic myomectomy (SIRM) and multiport robotic myomectomy (MPRM) and provide surgical tips. We retrospectively analyzed the medical records of 462 patients with symptomatic leiomyoma who underwent MPRM or SIRM between March 2019 and April 2021. Demographic characteristics and surgical outcomes, including the total operative time (OT), estimated blood loss (EBL), and surgical complication rate, were compared between the two groups. Patients in the SIRM group had lower a body mass index and rate of previous pelvic surgery and were younger than those in the MPRM group. The myoma type was not different between groups; however, the MPRM group had larger, and more myomas than the SIRM group. After propensity score matching, these variables were not significantly different between the groups. The total OT, EBL, difference in hemoglobin levels, transfusion rate, and postoperative fever were not different between the groups. No postoperative complications occurred in the SIRM group. In the MPRM group, one patient needed conversion to laparotomy, and two patients had postoperative complications (umbilical incisional hernia and acute kidney injury). In conclusion, both MPRM and SIRM are feasible and effective surgical options for symptomatic myomas with cosmetic benefits and minimal risk of laparotomy conversion.
Analysis of Neural Interface When Using Modiolar Electrode Stimulation. Radiological Evaluation, Trans-Impedance Matrix Analysis and Effect on Listening Effort in Cochlear Implantation
Authors: Ramos-de-Miguel Angel , Falcón-González Juan Carlos , Ramos-Macias Angel
Published: September 2021

Background: The proximity of the electrode to the modiolar wall may be of interest to investigate the effect of pitch discrimination. This research establishes the relation between these factors and whether perimodiolar positions may provide benefits regarding improved electrode discrimination. Methods: A prospective randomized study including 24 post-lingual deaf adults was performed. A psychoacoustic study was done by using a psychoacoustic research platform. Radiological study, and a cone-beam computed tomography was used to assess post cochlear implantation electrodes’ position. Trans-impedance matrix (TIM) analysis was performed after cochlear implant insertion in all cases, and pupillometry test was also performed. Results: 12 patients received a slim perimodiolar electrode array, and 12 patients received a straight electrode array. Although all the patients showed similar speech test results after 12 months follow-up, those implanted with a perimodiolar electrode obtained better scores in electrode discrimination test and pupillometry test, and showed more homogenous TIM patterns. Conclusions: The better positioning of the electrode array seams to provide a better hearing resolution and less listening effort trans-impedance matrix seems to be a useful tool to analyze positioning of the perimodiolar array.
Improvements in Gold Nanorod Biocompatibility with Sodium Dodecyl Sulfate Stabilization
Authors: Terracciano Rossana , Zhang Aobo , Simeral Mathieu L. , Demarchi Danilo , Hafner Jason H. , Filgueira Carly S.
Published: September 2021

Due to their well-defined plasmonic properties, gold nanorods (GNRs) can be fabricated with optimal light absorption in the near-infrared region of the electromagnetic spectrum, which make them suitable for cancer-related theranostic applications. However, their controversial safety profile, as a result of surfactant stabilization during synthesis, limits their clinical translation. We report a facile method to improve GNR biocompatibility through the presence of sodium dodecyl sulfate (SDS). GNRs (120 × 40 nm) were synthesized through a seed-mediated approach, using cetyltrimethylammonium bromide (CTAB) as a cationic surfactant to direct the growth of nanorods and stabilize the particles. Post-synthesis, SDS was used as an exchange ligand to modify the net surface charge of the particles from positive to negative while maintaining rod stability in an aqueous environment. GNR cytotoxic effects, as well as the mechanisms of their cellular uptake, were examined in two different cancer cell lines, Lewis lung carcinoma (LLC) and HeLa cells. We not only found a significant dose-dependent effect of GNR treatment on cell viability but also a time-dependent effect of GNR surfactant charge on cytotoxicity over the two cell lines. Our results promote a better understanding of how we can mediate the undesired consequences of GNR synthesis byproducts when exposed to a living organism, which so far has limited GNR use in cancer theranostics.
Pathophysiology of Takotsubo Syndrome as A Bridge to Personalized Treatment
Authors: Budnik Monika , Piątkowski Radosław , Ochijewicz Dorota , Zaleska Martyna , Grabowski Marcin , Opolski Grzegorz
Published: September 2021

Takotsubo syndrome (TTS) consists of transient dysfunction of the left and/or right ventricle in the absence of ruptured plaque; thrombus or vessel dissection. TTS may be divided into two categories. Primary TTS occurs when the cause of hospitalization is the symptoms resulting from damage to the myocardium usually preceded by emotional stress. Secondary TTS occurs in patients hospitalized for other medical; surgical; anesthetic; obstetric or psychiatric conditions who have activation of their sympathetic nervous system and catecholamines release- they develop TTS as a complication of their primary condition or its treatment. There are several hypotheses concerning the cause of the disease. They include a decrease in estrogen levels; microcirculation dysfunction; endothelial dysfunction and the hypothesis based on the importance of the brain-heart axis. More and more research concerns the importance of genetic factors in the development of the disease. To date; no effective treatment or prevention of recurrent TTS has been found. Only when the pathophysiology of the disease is fully known; then personalized treatment will be possible.
Molecular Classification Models for Triple Negative Breast Cancer Subtype Using Machine Learning
Authors: Bissanum Rassanee , Chaichulee Sitthichok , Kamolphiwong Rawikant , Navakanitworakul Raphatphorn , Kanokwiroon Kanyanatt
Published: September 2021

Triple negative breast cancer (TNBC) lacks well-defined molecular targets and is highly heterogenous, making treatment challenging. Using gene expression analysis, TNBC has been classified into four different subtypes: basal-like immune-activated (BLIA), basal-like immune-suppressed (BLIS), mesenchymal (MES), and luminal androgen receptor (LAR). However, there is currently no standardized method for classifying TNBC subtypes. We attempted to define a gene signature for each subtype, and to develop a classification method based on machine learning (ML) for TNBC subtyping. In these experiments, gene expression microarray data for TNBC patients were downloaded from the Gene Expression Omnibus database. Differentially expressed genes unique to 198 known TNBC cases were identified and selected as a training gene set to train in seven different classification models. We produced a training set consisting of 719 DEGs selected from uniquely expressed genes of all four subtypes. The highest average accuracy of classification of the BLIA, BLIS, MES, and LAR subtypes was achieved by the SVM algorithm (accuracy 95–98.8%; AUC 0.99–1.00). For model validation, we used 334 samples of unknown TNBC subtypes, of which 97 (29.04%), 73 (21.86%), 39 (11.68%) and 59 (17.66%) were predicted to be BLIA, BLIS, MES, and LAR, respectively. However, 66 TNBC samples (19.76%) could not be assigned to any subtype. These samples contained only three upregulated genes (EN1, PROM1, and CCL2). Each TNBC subtype had a unique gene expression pattern, which was confirmed by identification of DEGs and pathway analysis. These results indicated that our training gene set was suitable for development of classification models, and that the SVM algorithm could classify TNBC into four unique subtypes. Accurate and consistent classification of the TNBC subtypes is essential for personalized treatment and prognosis of TNBC.
Do All Coccidia Follow the Same Trafficking Rules?
Authors: Marugan-Hernandez Virginia , Sanchez-Arsuaga Gonzalo , Vaughan Sue , Burrell Alana , Tomley Fiona M.
Published: September 2021

The Coccidia are a subclass of the Apicomplexa and include several genera of protozoan parasites that cause important diseases in humans and animals, with Toxoplasma gondii becoming the ‘model organism’ for research into the coccidian molecular and cellular processes. The amenability to the cultivation of T. gondii tachyzoites and the wide availability of molecular tools for this parasite have revealed many mechanisms related to their cellular trafficking and roles of parasite secretory organelles, which are critical in parasite-host interaction. Nevertheless, the extrapolation of the T. gondii mechanisms described in tachyzoites to other coccidian parasites should be done carefully. In this review, we considered published data from Eimeria parasites, a coccidian genus comprising thousands of species whose infections have important consequences in livestock and poultry. These studies suggest that the Coccidia possess both shared and diversified mechanisms of protein trafficking and secretion potentially linked to their lifecycles. Whereas trafficking and secretion appear to be well conversed prior to and during host-cell invasion, important differences emerge once endogenous development commences. Therefore, further studies to validate the mechanisms described in T. gondii tachyzoites should be performed across a broader range of coccidians (including T. gondii sporozoites). In addition, further genus-specific research regarding important disease-causing Coccidia is needed to unveil the individual molecular mechanisms of pathogenesis related to their specific lifecycles and hosts.
4-(((4-Methoxyphenyl)amino)methyl)-N,N-dimethylaniline and 2-Methoxy-5-((phenylamino)methyl)phenol
Authors: Ajibade Peter A. , Andrew Fartisincha P.
Published: September 2021

Molecular structures of 4-(((4-methoxyphenyl)amino)methyl)-N,N-dimethylaniline and 2-methoxy-5-((phenylamino)methyl)phenol synthesized via Schiff bases reduction route are reported. The compounds consist of asymmetric units of C16H20N2O (1) and C14H15NO2 (2) in orthorhombic and monoclinic crystal systems, respectively. Compound 1 consist of intermolecular C11—H11···N2 hydrogen bonding with C11···N21 = 3.463(4) Å. The hydroxyl group in 2 is also involved in intermolecular O2—H2···O2 and O2—H2···O21 hydrogen bonding with O2···O11 = 2.8885(15) Å and O1···O21 = 2.9277(5) Å. The molecular structures of the compounds are stabilized by secondary intermolecular interactions of C1—H1B···O11 and C5—H···(C41, C51, C61, C71) for 1 and H···C, C—H···O and N—H···C for 2. The reported compounds are important starting material for the synthesis of many compounds such as azo dyes and dithiocarbamate.
Feedforward of Measurable Disturbances to Improve Multi-Input Feedback Control
Authors: Rico-Azagra Javier , Gil-Martínez Montserrat
Published: September 2021

The availability of multiple inputs (plants) can improve output performance by conveniently allocating the control bandwidth among them. Beyond that, the intervention of only the useful plants at each frequency implies the minimum control action at each input. Secondly, in single input control, the addition of feedforward loops from measurable external inputs has been demonstrated to reduce the amount of feedback and, subsequently, palliate its sideband effects of noise amplification. Thus, one part of the action calculated by feedback is now provided by feedforward. This paper takes advantage of both facts for the problem of robust rejection of measurable disturbances by employing a set of control inputs; a previous work did the same for the case of robust reference tracking. Then, a control architecture is provided that includes feedforward elements from the measurable disturbance to each control input and feedback control elements that link the output error to each control input. A methodology is developed for the robust design of the named control elements that distribute the control bandwidth among the cheapest inputs and simultaneously assures the prescribed output performance to correct the disturbed output for a set of possible plant cases (model uncertainty). The minimum necessary feedback gains are used to fight plant uncertainties at the control bandwidth, while feedforward gains achieve the nominal output response. Quantitative feedback theory (QFT) principles are employed. An example illustrates the method and its benefits versus a control architecture with only feedback control elements, which have much more gain beyond the control bandwidth than when feedforward is employed.
A Novel Approach to Generalized Intuitionistic Fuzzy Sets Based on Interpolative Boolean Algebra
Authors: Milošević Pavle , Petrović Bratislav , Dragović Ivana
Published: September 2021

One of the main issues in IFS theory are generalizations of intuitionistic fuzzy set (IFS) definition as well as IFS operations. In this paper, we present the LBIFS-IBA approach by applying operations based on interpolative Boolean algebra (IBA) on generalized IFS. Namely, LBIFS are defined as a special case of Liu’s generalized IFS with the maximal interpretational surface. By extending the interpretational surface, the descriptive power of the approach is enhanced, and therefore the problematic situations when μA+νA>1 can be modeled. In addition, IBA-based algebra secures Boolean properties of the proposed approach. Considerable attention is given to comprehension of uncertainty within LBIFS-IBA, i.e., we propose a novel manner of uncertainty interpretation by treating values from [−1,1] interval. In order to prove its importance, we compare LBIFS-IBA with several well-known IFS generalizations, showing that only our approach offers meaningful uncertainty interpretation is all selected cases. Additionally, we illustrate the practical benefits of LBIFS-IBA by applying it to an example of modeling Japanese candlesticks for price charting and paying special attention to uncertainty interpretation.
Pneumomediastinum and Pneumothorax as Relevant Complications of Sub-Intensive Care of Patients with COVID-19: Description of a Case Series
Authors: Coppola Maria Gabriella , Lugarà Marina , Tamburrini Stefania , Madonna Pasquale , Panico Claudio , Noschese Giuseppe , Pone Eduardo
Published: September 2021

Lung failure has been the most common cause of hospitalization for COVID-19. Yet, bilateral interstitial pneumonia has not been the only cause of lung failure of these inpatients, and frequently they develop other illnesses associated with COVID-19. Pulmonary embolism has been the most looked for in the world, but rarely other pneumological diseases, such as pneumothorax and pneumomediastinum, have been described and associated with a worsening prognosis. We here report our clinical experience associated with the occurrence of pneumothorax and pneumomediastinum in a cohort of inpatients hospitalized in our division of medicine in a regular ward or in a sub-intensive ward.
Impact of Periodontal Attachment Loss on the Outcome of Endodontic Microsurgery: A Systematic Review and Meta-Analysis
Authors: Sarnadas Margarida , Marques Joana A. , Baptista Isabel Poiares , Santos João Miguel
Published: September 2021

Background and Objectives: Endodontic microsurgery (EMS) aims to eradicate the sources of infection once the apical root resection removes most of the infected anatomical structures and repairs potential procedural errors in the apical region. An endodontic-periodontal lesion yields a pathological communication between the pulp and the periodontium. The purpose of this systematic review and meta-analysis is to evaluate the impact of periodontal attachment loss on the outcome of teeth submitted to EMS. Materials and Methods: PRISMA guidelines were followed. An electronic search was performed in EBSCOhost, Embase and PubMed databases with the following search key: (“endodontic microsurgery” AND “outcome”). No filters were used concerning the year of publication or language. Only randomized clinical trials, prospective and retrospective clinical studies in humans, with a minimum one-year follow-up, defined clinical and radiographic outcome criteria and estimable success rate for endodontic-periodontal lesion were included. Statistical analysis was performed using OpenMeta[Analyst] software. Results: Of a total of 113 articles, 34 were selected for full-text reading after duplicates deletion and title and abstract analysis. Thirteen and six studies were included in the systematic review and meta-analysis, respectively. A total of 2775 pooled teeth were submitted to EMS, of which 492 teeth and 4 roots had periodontal involvement. According to the qualitative analysis, success rates of the endodontic-periodontal group ranged from 67.6% to 88.2%. Meta-analysis revealed that the absence of periodontal attachment loss was predictive of a higher likelihood of success with an odds ratio of 3.14. Conclusions: Periodontal attachment loss presents a risk factor for EMS outcome. Although endodontic-periodontal lesions were associated with lower success rates considering a 1 to 10 years follow-up period, long-term successful prognosis following EMS has been reported, therefore presenting a fully valid and viable therapeutic option for the management of this type of lesions.
Nitrogen-Doped Porous Carbon Materials Derived from Graphene Oxide/Melamine Resin Composites for CO2 Adsorption
Authors: Ouyang Like , Xiao Jianfei , Jiang Housheng , Yuan Shaojun
Published: September 2021

CO2 adsorption in porous carbon materials has attracted great interests for alleviating emission of post-combustion CO2. In this work, a novel nitrogen-doped porous carbon material was fabricated by carbonizing the precursor of melamine-resorcinol-formaldehyde resin/graphene oxide (MR/GO) composites with KOH as the activation agent. Detailed characterization results revealed that the fabricated MR(0.25)/GO-500 porous carbon (0.25 represented the amount of GO added in wt.% and 500 denoted activation temperature in °C) had well-defined pore size distribution, high specific surface area (1264 m2·g−1) and high nitrogen content (6.92 wt.%), which was mainly composed of the pyridinic-N and pyrrolic-N species. Batch adsorption experiments demonstrated that the fabricated MR(0.25)/GO-500 porous carbon delivered excellent CO2 adsorption ability of 5.21 mmol·g−1 at 298.15 K and 500 kPa, and such porous carbon also exhibited fast adsorption kinetics, high selectivity of CO2/N2 and good recyclability. With the inherent microstructure features of high surface area and abundant N adsorption sites species, the MR/GO-derived porous carbon materials offer a potentially promising adsorbent for practical CO2 capture.
Efficient Direct Nitrosylation of α-Diimine Rhenium Tricarbonyl Complexes to Structurally Nearly Identical Higher Charge Congeners Activable towards Photo-CO Release
Authors: Nasiri Sovari Sara , Kolly Isabelle , Schindler Kevin , Cortat Youri , Liu Shing-Chi , Crochet Aurelien , Pavic Aleksandar , Zobi Fabio
Published: September 2021

The reaction of rhenium α-diimine (N-N) tricarbonyl complexes with nitrosonium tetrafluoroborate yields the corresponding dicarbonyl-nitrosyl [Re(CO)2(NO)(N-N)X]+ species (where X = halide). The complexes, accessible in a single step in good yield, are structurally nearly identical higher charge congeners of the tricarbonyl molecules. Substitution chemistry aimed at the realization of equivalent dicationic species (intended for applications as potential antimicrobial agents), revealed that the reactivity of metal ion in [Re(CO)2(NO)(N-N)X]+ is that of a hard Re acid, probably due to the stronger π-acceptor properties of NO+ as compared to those of CO. The metal ion thus shows great affinity for π-basic ligands, which are consequently difficult to replace by, e.g., σ-donor or weak π-acids like pyridine. Attempts of direct nitrosylation of α-diimine fac-[Re(CO)3]+ complexes bearing π-basic OR-type ligands gave the [Re(CO)2(NO)(N-N)(BF4)][BF4] salt as the only product in good yield, featuring a stable Re-FBF3 bond. The solid state crystal structure of nearly all molecules presented could be elucidated. A fundamental consequence of the chemistry of [Re(CO)2(NO)(N-N)X]+ complexes, it that the same can be photo-activated towards CO release and represent an entirely new class of photoCORMs.
Microwave-Assisted Ionic Liquid-Catalyzed Selective Monoesterification of Alkylphosphonic Acids—An Experimental and a Theoretical Study
Authors: Harsági Nikoletta , Henyecz Réka , Ábrányi-Balogh Péter , Drahos László , Keglevich György
Published: September 2021

It is well-known that the P-acids including phosphonic acids resist undergoing direct esterification. However, it was found that a series of alkylphoshonic acids could be involved in monoesterification with C2–C4 alcohols under microwave (MW) irradiation in the presence of [bmim][BF4] as an additive. The selectivity amounted to 80–98%, while the isolated yields fell in the range of 61–79%. The method developed is a green method for P-acid esterification. DFT calculations at the M062X/6–311+G (d,p) level of theory (performed considering the solvent effect of the corresponding alcohol) explored the three-step mechanism, and justified a higher enthalpy of activation (160.6–194.1 kJ·mol−1) that may be overcome only by MW irradiation. The major role of the [bmim][BF4] additive is to increase the absorption of MW energy. The specific chemical role of the [BF4] anion of the ionic liquid in an alternative mechanism was also raised by the computations.
Inhibitory Effect of Fisetin on α-Glucosidase Activity: Kinetic and Molecular Docking Studies
Authors: Shen Beiyun , Shangguan Xinchen , Yin Zhongping , Wu Shaofu , Zhang Qingfeng , Peng Wenwen , Li Jingen , Zhang Lu , Chen Jiguang
Published: September 2021

The inhibition of α-glucosidase is a clinical strategy for the treatment of type 2 diabetes mellitus (T2DM), and many natural plant ingredients have been reported to be effective in alleviating hyperglycemia by inhibiting α-glucosidase. In this study, the α-glucosidase inhibitory activity of fisetin extracted from Cotinus coggygria Scop. was evaluated in vitro. The results showed that fisetin exhibited strong inhibitory activity with an IC50 value of 4.099 × 10−4 mM. Enzyme kinetic analysis revealed that fisetin is a non-competitive inhibitor of α-glucosidase, with an inhibition constant value of 0.01065 ± 0.003255 mM. Moreover, fluorescence spectrometric measurements indicated the presence of only one binding site between fisetin and α-glucosidase, with a binding constant (lgKa) of 5.896 L·mol−1. Further molecular docking studies were performed to evaluate the interaction of fisetin with several residues close to the inactive site of α-glucosidase. These studies showed that the structure of the complex was maintained by Pi-Sigma and Pi-Pi stacked interactions. These findings illustrate that fisetin extracted from Cotinus coggygria Scop. is a promising therapeutic agent for the treatment of T2DM.