bluuebunny/crossref_metadata_2025 huggingface dataset:
Machine learning for ranking multivariate variables in cattle breeds raised in Paraguayan wetlands
Authors: Pereira Walter E. , Centurión Liz M. , Valdez Carolina , Martínez-López Roberto
Published: September 2024

ABSTRACT This study focuses on the performance of cows for meat production raised in the wetlands of Paraguay, examining five cattle genotypes: Brahman, Brangus, and Nelore, as well as two local breeds at risk of extinction. The main objective is to identify and rank phenotypic variables, including blood, clinical, hair, and health variables, demonstrating causal linkage with the live weight of the cows analyzed. Initially, high correlations were identified between different variables included in this study; then, using advanced Machine learning (ML) techniques and the application of Shapley additive explanations (SHAP), a deeper understanding was provided of the factors strongly associated with adaptability in these environments, and, therefore, the respective zootechnical performance. The association between cattle genotypic components linked with the season of the year proved to be the most influential factor on cattle live weight. Variables such as hair length, hematocrit, phosphatase, phosphorus, creatine phosphokinase, creatinine, protein, cortisol, calcium, and the presence of endoparasites were highlighted, demonstrating their hierarchical importance for animal selection. ML models are effective tools for establishing hierarchies of relevance in complex phenotypic multivariable, which is crucial in breeding programs for different zootechnical species and in special and specific environments like wetlands.
Evaluation of the behavior of Brazilian banks in merger and acquisition transactions
Authors: Angeli Allan Pétris , Rebehy Perla Calil Pongeluppe Wadhy , Salgado Junior Alexandre Pereira , Sardelari Júnior José Roberto , Bonaretti Filho Carlos Roberto
Published: September 2024

Abstract This study aims to determine the impact of an M&A on the performance of the consolidating bank in relation to its competitors in both the intermediation and profitability approaches, in addition to proposing an M&A ranking metric for a more accurate performance analysis. Although some studies assess the performance of banks in M&As, the explanation of their impact on institutions in the market can be better explored by proposing a financial-operational metric, different from valuations by the stock market, complemented with the point of view of organizational strategies that appear in the literature as essential for the continuity of the resourcefulness of the topic. Assessing the consequences of bank M&As and understanding their intentions provides better management of both institutions and the country's socioeconomic aspects. It also provides guidelines and new tools for performance evaluation for academics, managers, and government officials on M&A processes. The research method is quantitative-qualitative. In the quantitative approach, the data envelopment analysis (DEA) technique with a second stage of multiple linear regression was used. The database was formed by information from the banks' financial statements from 2000 to 2018 (representing the CAMELS), supplemented with macroeconomic and market structure information. In the qualitative approach, the impact of these regressed variables was determined in the quantitative stage for four case studies, which are the Big4 banks that represent approximately 87% of the Brazilian financial market. Although some studies deal with the topic of the relationship between efficiency and M&As, this study shows that there is no clear and direct relationship between M&As and the performance (efficiency) of banks in the market. The results suggest that banks carry out M&As for reasons other than the search for efficiency, leaving efficiency as a consequence of the operation. This article contributes to the proposal of a new metric for measuring the efficiency of M&As, whose function is to establish a ranking and relativize the analysis of banks' performance in relation to the market as a whole, allowing the visualization of the relative effect vis-à-vis competitors.
Porcine Circovirus Type 2 Genotypes and PCV3 in Swine Clinical Samples From Brazil
Authors: Miotto Rovian , Pissetti Caroline , Bordin Luiz Carlos , Zanella Janice Reis Ciacci
Published: September 2024

Abstract Porcine circovirus type 2 (PCV2), an important pathogen in swine, causes PCV disease (PCVD). Although PCVD is effectively controlled using commercial vaccines, its clinical presentation is changing. Moreover, PCV2 is genetically evolving, with new genotypes emerging in vaccinated or unvaccinated pigs. In this study, we aimed to verify the presence of the PCV2a, PCV2b, and PCV2d genotypes in PCV-positive porcine samples. Furthermore, to identify coinfections between the PCV2 genotypes and/or PCV3, which can also induce disease in pigs, we employed a quick, effective, and low-cost PCR diagnostic test. In this study, 333 PCV2 PCR and clinically positive samples from various production stages and herds across Brazil were analyzed. Among these, 266 samples were genotyped, with PCV2b emerging as the most predominant genotype (56.77% of the positive samples), mainly observed in nursery pigs. PCV2d was also identified in 33.10% of the samples, primarily from finishing pigs and breeding sows. The employed PCR test was compared with a commercial kit, proving effective in PCV2 genotyping. This study demonstrates the significance of PCV2 genotyping, showing PCV2b as the most predominant genotype responsible for disease in pig farms in Brazil. PCV2a, the prevalent genotype used in commercial vaccines, was not detected in any of the analyzed samples. While pigs infected with other PCV2 genotypes may receive some heterologous protection from PCV2a vaccines, adequate diagnosis and vaccine monitoring for updates must be considered.
Substituição de virginiamicina por produtos à base de levadura (Saccharomyces cerevisiae) em dietas de bovinos: Digestibilidade e parâmetros ruminais
Authors: Oliveira Daiana dos Santos de , Silva Luan de Oliveira Moura , Ribeiro Laís Gonçalves , Sousa Debora Gomes de , Pereira Leidiano Martins , Couto Victor Rezende Moreira , Fernandes Marcia Helena Machado da Rocha , Arnhold Emmanuel , Nascimento Mariana Quintino do , Manzano Ricardo Pereira , Fernandes Juliano José de Resende
Published: September 2024

Resumo O objetivo do estudo foi avaliar a substituição de virginiamicina por produtos à base de levedura em dietas de alto grão contendo monensina, sobre a digestibilidade dos nutrientes e parâmetros ruminais. Cinco novilhos cruzados Nelore x Holandês (peso inicial 281 kg ± 9,2 kg) distribuídos em um delineamento Quadrado Latino 5 x 5. As dietas foram compostas por silagem de milho (355 g kg-1) e concentrado composto por: milho moído, farelo de soja, casca de soja, mistura mineral, calcário, sal e ureia (645 g kg-1). Os tratamentos foram: 1. Controle, 18 mg kg-1 MS de virginiamicina (VM); 2. Cultura de levedura enriquecida (Saccharomyces cerevisiae) 7g animal dia-1 (CLE 7); 3. Cultura de levedura enriquecida (Saccharomyces cerevisiae) 14g animal dia-1 (CLE 14); 4. Levedura autolisada (Saccharomyces cerevisiae) 7g animal dia-1 (LA 7) e Levedura autolisada (Saccharomyces cerevisiae) 14g animal dia-1 (LA 14). A monensina (18 mg kg-1 MS) estava presente na mistura mineral vitamínica fornecida em todos tratamentos. Não houve diferença significativa (P>0,05) para o consumo de matéria seca (média 8,36 kg MS); digestibilidade da matéria orgânica (média 58,39%) e média de pH ruminal (média 6,75). Além disso, não foram encontrados efeitos significativos de tratamentos para concentração de ácidos graxos de cadeia curta e nitrogênio amoniacal. Estes resultados indicam a possibilidade de substituir a virginiamicina por produtos à base de levedura nos menores níveis (7g animal dia-1 de cultura de levedura enriquecida ou 7g animal dia-1 de levedura autolisada) em dietas de alto grão com monensina.
Atributos produtivos e nutricionais da forragem hidropônica de milho, em diferentes estágios, cultivada em substratos de capim-elefante e serragem de madeira
Authors: Cruz-Neiva Tais Borges da , Cerqueira Julia de Castro , Santos Emilly Sabrina Cotrim dos , Almeida Raniere de Araújo Lima , Silva Hackson Santos da , Candeias-Oliveira Eliane Leal , Andrade Sobrinho Luiz Edmundo Cincurá de , Oliveira Kaliane Nascimento de , Santana Ana Lúcia Almeida
Published: September 2024

Resumo O objetivo foi avaliar atributos produtivos e nutricionais da forragem hidropônica de milho, em diferentes estágios, cultivada em substratos de capim-elefante e serragem de madeira. Foram estabelecidos dois tratamentos (T), com diferentes substratos: T1 - capim-elefante picado e desidratado e T2 - serragem de madeira. No 13º e 20º dias após a semeadura, amostras da forragem foram coletadas para avaliação do desenvolvimento das plântulas, produção de biomassa e composição nutricional. O substrato capim-elefante promoveu melhor desenvolvimento das plântulas, maior produção de biomassa e concentração de matéria mineral, aos 13 dias, quando a serragem de madeira promoveu maior concentração de matéria seca, proteína bruta, lignina e carboidrato não fibrosos (P<0,05). O substrato capim-elefante resultou em maior altura de plântula, produção de biomassa verde, concentração de matéria mineral, proteína bruta e extrato etéreo, aos 20 dias, quando a serragem de madeira promoveu maior comprimento de raíz, maior concentração de matéria seca, fibra em detergente ácido e lignina (P<0,05). O substrato capim-elefante e a coleta aos 13 dias promoveram melhor resultado para a produção de forragem hidropônica, bem como para a sua composição nutricional.
RATES AND CAUSES OF OUTPATIENT SURGICAL CANCELLATIONS: A CROSS-SECTIONAL STUDY
Authors: Pinheiro Liliane Duarte Pereira Silva , Fassarella Cintia Silva , Carmo Thalita Gomes do , Camerini Flavia Giron , Luna Aline Affonso , Henrique Danielle de Mendonça , Ribeiro Olga Maria Pimenta Lopes , Pereira Soraia Cristina de Abreu
Published: September 2024

ABSTRACT Objective: to analyze the rate and causes of cancellations in an outpatient surgery unit at a university polyclinic that is part of the Unified Health System and located in the city of Rio de Janeiro. Method: a cross-sectional study carried out in the outpatient surgical center of a university polyclinic in the city of Rio de Janeiro, Brazil. The data for the period August 2021 to July 2022 was extracted from the institution's database using a form containing the patients' sociodemographic and clinical variables, month, surgical specialty, causes and period of cancellation. The Wilcoxon-Mann-Whitney test was used for the age group variable, and Pearson's chi-square test was used for the month and specialty variables, with a significance level of 5%. Results: of the 2,147 outpatient surgeries scheduled, 334 were canceled, with an annual surgical cancellation rate of 15.55%. There was a statistically significant difference in cancellations in December (p= 0.010), in the vascular surgery specialty (p= 0.001) and in older adults (p= 0.007). A total of ten causes of cancellation were found, the most frequent being patient absence (n=117; 35.03%), unfavorable clinical conditions (n=92; 27.54%) and non-compliance with preoperative preparation (n=30; 8.98%). Conclusion: the surgical cancellation rate was high, mainly due to the patient's absence and clinical conditions on the day of surgery. It is hoped that the data will help to subsidize and raise awareness of the active participation of all professionals involved in outpatient surgery, in order to avoid cancellations.
Association between bullying victimization and health risk behavior in adolescents
Authors: Pacífico Ana Beatriz , Bacil Eliane Denise Araújo , Ardengue Mariana , Piola Thiago Silva , Silva Michael Pereira da , Fontana Fabio , Avelar Ademar , Campos Wagner de
Published: September 2024

ABSTRACT Objective: The aim of this study was to examine the association between bullying victimization and health risk behaviors in adolescents. Methods: A representative sample of 1020 adolescents participated in the study. The variables such as bullying, health risk behaviors (tobacco, drugs, alcohol, sedentary behavior, smartphone use, level of physical activity, and sleep), and economic status were assessed using self-reported questionnaires. Odds ratios with 95% confidence intervals (95%CI) were obtained using binary logistic regression and ordinal, gross, and adjusted logistic regression (p<0.05). Results: Victims of bullying were more likely to smoke (OR 1.75; 95%CI 1.28–2.40), consume alcohol (OR1.43; 95%CI 1.05–1.94), have worse sleep quality (OR 1.94; 95%CI 1.28–2.91), and more sedentary behavior (OR 1.43; 95%CI 1.08–1.89) than those who were not bullied. However, victims were more likely to have high levels of physical activity than their non-bullied peers (OR 1.66; 95%CI 1.22–2.27). Conclusions: Bullying victimization was associated with an increased predisposition for the adoption of health risk behaviors. Interestingly, victims were also more prone to participate in physical activity.
A Constituinte de 1823: ideologia e historiografia
Authors: Lynch Christian Edward Cyril
Published: September 2024

Resumo A celebração do bicentenário do constitucionalismo brasileiro incentiva uma reflexão sobre a relação entre a história profissional e a memória coletiva, destacando como a última é frequentemente moldada por ideologias. A historiografia ajuda a identificar essas visões ideológicas e pode contribuir para uma análise mais equilibrada dos eventos históricos. O presente artigo examina a Constituinte de 1823 como estudo de caso. Durante seu funcionamento, emergiram três correntes ideológicas que explicaram de forma distinta os acontecimentos da época e se cristalizaram depois na forma de historiografias partidárias - conservadora, liberal e radical -, cujos ecos ainda hoje se fazem ouvir. O estudo da historiografia revela como as ideologias e tradições partidárias se mantêm ao longo do tempo, transformando a história em um campo de disputa política em que diferentes perspectivas competem para validar suas interpretações dos eventos.
Qualidade de vida, estresse, enfrentamento e sobrecarga de cuidadores de pessoas idosas com Alzheimer
Authors: Lin Chia Chen , Marques Izabela Vitória Pereira , Silva Grazieli Covre da , Silva Eduardo Quadros da , Colombari Agnes Vitório , Nascimento Junior José Roberto Andrade do , Oliveira Daniel Vicentini de
Published: September 2024

Resumo Introdução O cuidado à pessoa idosa com doença de Alzheimer pode gerar um estado de estresse prolonga-do, principalmente quando a sobrecarga é intensa, o que impacta a qualidade de vida do cuidador. Objetivo Comparar a qualidade de vida, percepção de estresse e estratégias de enfrentamento em função do nível de sobrecarga de cuidadores de pessoas idosas com Alzheimer. Métodos Trata-se de um estudo quantitativo e transversal realizado com 126 cuidadores de pessoas idosas com Alzheimer. O World Health Organization Quality of Life Bref (WHOQOL-Bref), a Escala de Estresse Percebido, o Inventário de Sobrecarga de Zarit e a Escala de Modos de Enfrentamento de Problemas foram utilizados. A análise dos dados foi realizada por meio do teste de Kolmogorov-Smirnov, procedimentos de bootstrapping e Anova one-way seguida por post-hoc de Tukey. Adotou-se significância de p < 0,05. Resultados Cuidadores com sobrecarga intensa apresentaram pior qualidade de vida (p < 0,001). Cuidadores sem sobrecarga apresentaram menor estresse (p < 0,001) e utilizavam estratégia de enfrentamento focada no problema (p < 0,001). Conclusão O nível de sobrecarga é um fator interveniente na qualidade de vida, sintomas de estresse e estratégias de enfrentamento de cuidadores de pessoas idosas com Alzheimer.
O CONSUMO, A VIDA URBANA E A VIDA DIGITAL: DESDOBRAMENTOS ENTRE O EXPERENCIAR E O CONSUMIR
Authors: Saciloto Paulon Thainá , De Melo Machado Morgana
Published: September 2024

Em outras das nossas muitas andanças por Santa Maria/RS, duas antropólogas precisaram ir a um shopping da cidade e retornam às suas raízes acadêmicas e a uma tarefa etnográfica para este estudo. As reflexões iniciais surgiram a partir de ausências: por que o consumo deixou de ser um foco central na sociologia e antropologia? Nossas práticas de consumo urbano ainda persistem? O que a nova dinâmica de viver online e o universo da internet têm a nos ensinar? O consumo envolve um contato com a materialidade, através da percepção e dos sentidos, em uma experiência que é estruturada e guiada por esses sentidos. Por meio de uma constatação antropológica, voltamos nossa atenção, mais uma vez, para um consumo com novas facetas entre o material e o imaterial. Propomos uma categoria chamada “experiencialidade” para pensar os novos processos de consumo mediado pelas redes e telas, transitando entre o imaterial e o material nos processos contemporâneos de consumo, compondo um emaranhado de signos e significados que, por sua vez, nos proporciona uma nova perspectiva sobre o ato de consumir.
Science News Agencies in SciComm: an exploratory index for evaluating and enhancing public interest in mass-distributed press releases
Authors: Batista de Oliveira Monique , Hafiz Mariana , Fleerackers Alice , Bolonha Nunes Luiá , Fernandes Barata Germana
Published: September 2024

Scientific press releases are reaching the public directly through press reproduction or institutional dissemination. Science News Agencies (SNAs) mediate this process, distributing texts to thousands of journalists while also "leaking" them on their websites and social media. This comparative case study examines four SNAs — BORI, SMC UK, AlphaGalileo, and EurekAlert! — regarding their role in circulating public scientific information. Through literature review and principles such as openness and inclusion in science, we converted scholars' concerns into a preliminary index potentially capable of assessing SNAs' public suitability. The SNAPI (Science News Agencies Public Index) suggests a shift from purely public relations content towards serving the public interest, highlighting areas needing attention in SNAs' social function, to be refined in future research. Clear guidelines, links to open scientific articles, and explicit notices on press releases’ purposes are simple yet effective ways to address issues concerning science public relations' pervasiveness in the public sphere.
Analysis of New Hemogram Markers in Patients Presenting to the Emergency Department with Epileptic Seizures
Authors: Çaltekin İbrahim , Aygun Ali , Tomakin Mesut , Yazıcı Ramiz , Albayrak Güneş Seda
Published: September 2024

Objective: We aimed to analyze the diagnostic value of new potential hemogram biomarkers in patients presenting to the emergency department with epileptic seizures.
Materials and Methods: Patients presenting with epileptic seizures to a tertiary hospital's emergency department between January 1, 2023, and January 31, 2024, were retrospectively reviewed. The patients were categorized into Group A (seizure patients) and Group B (healthy volunteers). Hemogram and biochemistry data from routine blood tests were examined. To predict seizure status, ROC analysis was performed to evaluate NLR, PLR and PNR. The area under the curve and cutoff values were calculated for each marker.
Results: Our study examined two groups: seizure patients and the control group. The mean age of the seizure group was 50.31±1.83 years, and the mean age of the control group was 49.58±1.96 years (p>0.05). In the seizure group (n=116), 55.8% were male and 44.2% were female, whereas in the control group (n=104), 57.8% were male and 42.2% were female (p>0.05). WBC, neutrophil count, NLR, and PLR values were significantly higher in seizure patients compared to the control group (p
La dicotomía “acusatorio/inquisitivo” en el contexto colombiano: un camino hacia una transformación eficiente de la Procuraduría General de la Nación
Authors:   Universidad Libre, Gerenas Adriana , Cárdenas Fernando ,   Universidad  Militar  Nueva  Granada
Published: September 2024

La Ley 2094 de 2021 nos presenta un reto: garantizar el cumplimiento del principio de imparcialidad dentro de los procesos sancionatorios administrativos. Para hacer frente a ello, se visualiza como posible solución la aplicación de la separación de funciones, en el estricto sentido en que esta es asegurada por el principio acusatorio (propio del derecho penal). Dicha solución surge gracias a que, como resultado del análisis histó-rico-ontogenético, encontramos una analogía entre los orígenes del principio acusatorio y de la Ley 2094 de 2021; a su vez, esta analogía se encuentra soportada conceptualmente en la dicotomía “acusatorio/inquisi-tivo”. Con el propósito de evaluar la viabilidad de esta propuesta, se realiza un análisis lógico con el que se verifica que no existe inconsistencia entre la lógica jurídica de los dos principios. Con base en lo anterior, se evalúa la consistencia lógica de la aplicación misma del principio acusatorio al marco normativo que esta-blece la Ley 2094 de 2021. Finalmente, se expondrán las consecuencias lógicas que esta aplicación tendría y cómo respondería a las garantías exigidas a la Procuraduría General de la Nación por parte de la Corte Interamericana de Derechos Humanos.
Caracterización y prospectiva del ejercicio profesional de los médicos veterinarios, médicos veterinarios zootecnistas y de zootecnistas en Colombia
Authors:   Asociación Colombiana de Facultades de Medicina Veterinaria y Zootecnia, Bedoya Mashuth Julia Teresa
Published: September 2024

Información del Ministerio de Educación Nacional muestra un creciente número de profesionales en Medicina Veterinaria, Medicina Veterinaria y Zootecnia, y Zootecnia. Sin embargo, esta información ha sido objeto de análisis fragmentados. El impacto de la pandemia por COVID-19 en 2020 generó nuevas dinámicas en las actividades académicas y profesionales, razón por la cual la Asociación Colombiana de Facultades de Medicina Veterinaria y Zootecnia (ASFAMEVEZ) llevó a cabo un estudio nacional para comprender mejor las dinámicas educativas y laborales en estas áreas, con el objetivo de caracterizar el estado, evolución y factores influyentes en el ejercicio profesional y bienestar de estos profesionales. La investigación se realizó mediante una metodología transversal descriptiva, que incluyó tres encuestas estructuradas, aplicadas entre enero y mayo de 2023 a estudiantes, graduados y empleadores. Como resultado, se obtuvo la participación de 2179 encuestados de las tres poblaciones, previamente avaladas por el Comité de Ética de la Universidad de La Salle. Los resultados revelan una realidad cercana de estas profesiones en Colombia, destacando el aumento de mujeres en medicina veterinaria y zootecnia. Aunque las percepciones son mayormente positivas, existe preocupación por el aspecto socioemocional. Se reconoce la importancia de estas disciplinas para diversos sectores, y se identifica el posgrado como clave para mejorar las condiciones socioeconómicas y competencias profesionales. Las condiciones laborales muestran tendencias similares entre las tres profesiones, siendo moderadas en general, pero potencialmente preocupantes en algunos casos, especialmente ante la creciente competencia de otras disciplinas. A pesar de estos desafíos, se vislumbra un futuro prometedor para estas profesiones en Colombia, aunque el impacto de la pandemia en los estudiantes ha sido variable.
¿Qué es escultura? Límites y expansiones en el campo del arte ecuatoriano. Caso 7ma Bienal Nacional de Escultura San Antonio de Ibarra
Authors: García-Moreno Mario Fernando , Paredes-Lascano Iliana Sabina
Published: September 2024

Los autores de este artículo han investigado los conceptos fundacionales de la escultura, y han examinado el proceso de transformación que esta ha experimentado desde finales del siglo xix. Tal transformación, que en su momento significó la ampliación de los recursos creativos de la escultura tradicional, dio lugar a inéditas propuestas y obras que, si bien a la larga fueron consagradas por la institución artística, en su momento afrontaron la incomprensión de amplios sectores sociales teóricamente ignorantes (Dickie, 1969) y visualmente analfabetos (Dondis, 1973), a su juicio. Por inercia, en la actualidad no son pocas las voces que se oponen a las manifestaciones artísticas que adoptan y exploran la llamada escultura expandida, así como son numerosas aquellas otras que, aunque instruidas, la confunden con categorías colindantes que comparten el campo en que esta se ubica (Krauss, 1979). Para fines educativos, sociales e institucionales, este artículo proporciona un marco teórico que permite mediar entre tradición y expansión, ante posibles conflictos generados cuando se convoca a la comunidad del arte a participar en procesos educativos y eventos culturales donde se menciona la palabra escultura, tal como sucede en la localidad de San Antonio de Ibarra y su Bienal Nacional.
Indirect Co-Culture Assay using Boyden Chambers v1
Authors: Cruz Pachane Bianca , Sobreiro Selistre de Araujo Heloisa
Published: September 2024

Here, we describe a transwell assay using Boyden chambers as an indirect co-culture method, where two cell lines are grown together but separated by a porous membrane. This assay was used to determine the effect of hypoxic, tumoral EVs in cells from the tumor microenvironment without direct contact between them, focusing on intercellular adhesion and tumoral invasion.
R2C2 protocol using 10x Single-Cell RNA-seq AssaycDNA v1
Authors: Adiconis Xian , Z Levin Joshua
Published: September 2024

This protocol is based on the published Rolling Circle to Concatemeric Consensus (R2C2) method (1). The aim is to make elongated cDNA via rolling circle amplification using cDNA generated from the 10x Chromium 3' (or 5') single-cell RNA-seq assay to increase the accuracy of downstream sequencing on an Oxford Nanopore Technologies sequencing platform. The major modification from the original protocol is to add an additional cDNA re-amplification step (see section "10x cDNA re-amplification") to enrich for fragments with the proper sequence (poly(T) at 3' and TSO at 5') at both ends, which is essential in circularization with the 10x Splint fragment.
In vitro preparation of single filamentous microtubules optimized for dynamic and electrophoretic light scattering measurements. v1
Authors: George Annitta
Published: September 2024

The lack of essential information on sample preparation and the need to characterize and understand microtubules (MTs) in various biological functionalities within the cell have led us to develop a high-quality polymerization protocol to prepare fully formed MTs in vitro, which is particularly designed for Dynamic light scattering (DLS) and Electrophoretic light scattering (ELS) experiments for further analysis. This protocol details the reconstitution of tubulin, GTP, and Taxol, the preparation of PEM, cushion buffer, and polymerization buffers, the step-by-step polymerization process, and the use of Taxol to stabilize the microtubule filaments without precipitation. This polymerization protocol successfully generated samples for light scattering experiments using buffers that replicate physiological conditions. This has provided consistency in preparing stable, diluted, aggregate-free, homogenous microtubule filament samples that could benefit many other scientific research groups currently working in the field. Additionally, it can easily be adapted to prepare samples using other buffers and biological fluids. Graphical abstract: This sample preparation protocol requires 3 days. All the buffers and solutions are prepared to make enough of four samples of fully developed microtubules in 100 millimolar concentration of potassium chloride (KCl), with a total volume of 1 milliliter of solution. (See Materials sections).
INSIDEN PENURUNAN BANNER PEOPLE’S JUSTICE DI DOCUMENTA FIFTEEN DAN PERINTANG KOMUNIKASI ANTARBUDAYA
Authors:   MINDSET Institute, Heron Heronimus , Kim Min Seong ,   Program Pascasarjana Kajian Budaya, Universitas Sanata Dharma
Published: September 2024

On 20 June 2022, Indonesian artists and art observers were shocked by the news that Taring Padi’s banner artwork entitled People’s Justice exhibited at Documenta Fifteen was interpreted by protesters as containing anti-Semitic elements. Taring Padi explained that his work was based on Indonesia’s political landscape during the New Order regime and had nothing to do with anti-Semitism. However, the banner was eventually dismantled on 21 June 2022. This brought up several interesting research questions pertaining to the chronological account of the incident, the causes of the incident, and actions that should have been taken by Taring Padi as a cultural agent. This research applied the qualitative method with critical discourse analysis approach. Results found that the incident occurred because of two main reasons. Firstly, the protesters were unwilling to ask the artist for any clarification and explanation. Secondly, Taring Padi was ignorant about elements which could be considered anti-Semitic because the concept is not particularly well-known in Indonesia. People’s Justice could actually serve as a medium for initiating dialogues between fellow artists and their audience. However, this work has potential drawbacks because it contains potentially misunderstood antagonism that narrows the interpretive space. This incident could have been prevented if the artist had provided sufficient room for dialogic interpretation and if the audience had been more open-minded, through which discursive encounters can be held to understand each other’s cultural and societal problems.
Стереотаксические Атласы мозга животных и человека: справочник.
Authors: Буданцев А.Ю.
Published: September 2024

Справочник содержит информацию о 82-х Стереотаксических Атласах мозга животных и человека. Дополнительно приведен материал о Цитоархитек-тонических Атласах мозга и Работах в области визуализации структур мозга. Справочник предназначен для студентов, аспирантов и научных сотрудников, работающих в области экспериментальной физиологии мозга.
Epilepsy surgery for tuberous sclerosis complex in children: literature review and clinical case
Authors: Berdinov F. B. , Kuznetsova A. A. , Levov A. V. , Solovev V. B. , Russkin V. O. , Shchederkina I. O. , Livshits M. I. , Chmutin G. E.
Published: September 2024

Tuberous sclerosis complex (TSC) is a multisystem, autosomal-dominant, neurocutaneous syndrome that is characterized by the presence of hamartomas involving multiple organs, including the brain. Epilepsy is the most common neurological manifestation and the main cause of disability in children. Drug-resistant epilepsy is seen in 62.5 % of cases. The challenge of surgical treatment in these patients is the multifocal nature of epilepsy. Nonetheless, there is available data to suggest that surgical intervention is most likely to achieve long-term seizure freedom.The aim of the work – to analyze current data and aspects of surgical treatment of epilepsy associated with tuberous sclerosis in children.A literature search for was done on PubMed, Google Scholar, and eLIBRARY. RU for the period from 2000 to 2022. Search phrases included: TSC-associated epilepsy in children, epilepsy surgery in children with TSC, epilepsy surgery for TSC. The tubers are not the only source of epileptic activity; the perituberal brain tissue is also a proven focus. Currently, there is a tendency towards early pre-surgical evaluation and surgical treatment, which is recommended after the failure of two antiepileptic drugs. Considering the multiple brain lesions and multifocal epilepsy, the use of invasive electroencephalography is invaluable in the preoperative assessment of these patients. The effectiveness of resection surgery is 65–75 %. Over time, the proportion of patients in complete remission from seizures decreases. Lobectomy and tuberectomy plus procedures are favorable prognostic factors. Surgical treatmentsignificantly increasesthe chances of seizure freedom. Eliminating seizures in children has been shown to improve cognitive development.There is no algorithm for pre-surgical patient evaluation or selection criteria for surgical treatment. Some methods of presurgical evaluation are not included in the compulsory health insurance system, making early diagnosis and treatment very difficult. This leads to an increase in the number of patients with disabilities and a poor quality of life.
Sling-technique for vertebral artery transposition in neurovascular conflicts caused by dolichoectasia
Authors: Stanishevskiy A. V. , Svistov D. V. , Rafaelian A. A.
Published: September 2024

Vertebrobasilar dolichoectasia is rare but in symptomatic cases thoroughly reduce quality of life and is challenging for treatment. Current paper presentstwo cases of vertebral dolichoectasia that cause neurovascular compression syndromes. In both cases vertebral artery transposition performed using sling-technique. The aim of the study – to demonstrate special features and efficiency of sling-technique for neurovascular conflicts caused by dolichoectasia presented.Surgical video demonstrating particular technique also performed. Additionally, literature data on epidemiology, symptoms and surgical interventions for neurovascular conflicts caused by dolichoectasia presented.
Mindful Learning: Principles and Prospect of Use in Higher Education
Authors: Bordunos A.K. , Miletich M.P. , Volkova N.V.
Published: September 2024

The article provides an overview of the principles of mindful learning in the context of higher education. Mindful learning is an approach in which students and teachers tune their attention to increasing value and awareness of what is happening in the surrounding context, to their experience and reflection about it. The relevance of the research is related to the interest in the effects associated with mindful learning: positive impact on psychological well-being, development of socio-emotional skills, as well as increasing intrinsic motivation, improving attentiveness and concentration of students. The article reviews research indicating the effectiveness of this approach for both learning outcomes and the psychological well-being of students and teachers in the educational process. The theoretical contribution of this research lies in highlighting the research agenda in the field of mindful learning: defining concepts of mindful learning and mindful teaching, identifying the main principles of mindful learning. These results provide a basis for further research of mindful learning in higher education. The practical contribution of the research lies in presenting a methodological basis for introducing mindful learning into university disciplines. The research may be of interest not only to teachers and methodologists but also to students and their parents due to the increased interest in the effects of mindful learning.
Psychological State of Russian Student Youth in the Crisis: The Relationship with Cultural and Political Patriotism
Authors: Murashcenkova N.V.
Published: September 2024

The analysis of markers and determinants of the current psychological state of Russian youth as a risk group with high probability of combined influence on its state of normative crisis of identity and complex external conditions is important and necessary. In the study we conducted a hierarchical regression analysis of the correlation between the severity of psychological trauma associated with the special military operation and with the perception of the future of Russia (dependent variables) and cultural and political patriotism and several control variables (predictors). University students from Moscow, Omsk, Penza, Saratov, Smolensk, Khabarovsk (718 Russian citizens ages 18 to 25, M=19,4; SD=1,46, 21,6% of men) participated in the study. Data were collected in October 2022. We used the Russian version of “International Trauma Questionnaire (ITQ)” and questions from the International Social Survey Programme (ISSP 2013). We found signs of psychological trauma in the third of respondents. The link between the psychological condition of students and cultural and political patriotism and some control variables has been revealed. Cultural patriotism, combined with a low level of political patriotism, has contributed to the psychological trauma of young people in the current crisis. Cultural patriotism combined with political patriotism is a factor of positiv perception of the future of Russia. Based on the results, we presented a portrait of student who is in great need of psychological help.
Gap junction protein connexin 43 and its distribution in damaged nerve cells
Authors: Kolos Elena A. , Korzhevskii Dmitrii E.
Published: September 2024

BACKGROUND: Connexins, particularly connexin 43, form gap junctions that mediate neuron-glial communication. To date, the expression of connexin 43 in phenotypically distinct neurolemmocytes, in particular non-myelinating (Remak cells) and repair Schwann cells, has not been studied.
AIM: To determine the presence of connexin 43 in Schwann cells of the rat sciatic nerve under normal conditions and after mechanical damage by applying a ligature.
MATERIALS AND METHODS: The study was carried out on Wistar rats (n = 10). In experimental rats, the sciatic nerve was damaged by applying a ligature (40 s). 7 days after surgery, sciatic nerve segments were isolated for subsequent immunohistochemical study using antibodies to connexin 43 and glial fibrillary acidic protein (GFAP). In the control group rats, segments of intact sciatic nerves were isolated in a similar way.
RESULTS: It has been shown that in the endoneurium of the intact rat sciatic nerve there are no cells expressing connexin 43. It was found that 7 days after injury, a large number of connexin-43-immunopositive cells of irregular shape with several processes were identified in the endoneurium of the damaged nerve. There was a lack of expression of connexin 43 in GFAP-containing Schwann cells.
CONCLUSIONS: It can be stated that nerve damage leads to active synthesis of the studied protein by endoneurium cells; however, the origin of the cells expressing connexin 43 remains to be elucidated.
Regulatory cytokine network and macrophages (in memory of Irina S. Freidlin)
Authors: Kisseleva Ekaterina P. , Polevshchikov Alexander V.
Published: September 2024

Irina S. Freidlin was an outstanding Soviet and Russian immunologist, Doctor of Medical Sciences, Professor, Honored Scientist, Corresponding Member of the Russian Academy of Sciences. She was a natural for teaching and a deep scientific researcher whose interests were mainly related to the study of innate immunity.
Precariously Balanced Rocks in Northern New York and Vermont, U.S.A.: Ground-Motion Constraints and Implications for Fault Sources
Authors: McPhillips Devin , Pratt Thomas L.
Published: September 2024

Precariously balanced rocks (PBRs) and other fragile geologic features have the potential to constrain the maximum intensity of earthquake ground shaking over millennia. Such constraints may be particularly useful in the eastern United States (U.S.), where few earthquake-source faults are reliably identified, and moderate earthquakes can be felt at great distances due to low seismic attenuation. We describe five PBRs in northern New York and Vermont—a region of elevated seismic hazard associated with historical seismicity. These boulders appear to be among the most fragile PBRs in the region, based on reports from hobbyists. The PBRs are glacial erratics, best evidenced by glacial striations on bedrock pedestals. The pedestals themselves are locally high knobs, often situated on regionally high topography; this setting limits soil development and indicates that any outwash deposits were likely ephemeral. As a result, PBR ages can be reliably established by the retreat of the last continental ice sheet, ∼15–13 ka. To quantify the fragility of the PBRs, we surveyed them with ground-based light detection and ranging and calculated geometric parameters from the point clouds, field observations, and seismic responses. Preliminary validation of the 2023 time-independent U.S. National Seismic Hazard Model (NSHM) shows that the existence of PBRs is generally consistent with the median site-specific hazard curves. Only the Blue Ridge Road site suggests a modest reduction in hazard. To visualize the ensemble of data, we mapped the minimum permissible distance to potential source faults around each PBR site as a function of source magnitude by using the ground-motion models from the 2023 NSHM. Viewed in this manner, our data are consistent with potential M∼6.5 earthquake-source faults in many parts of the Lake Champlain Valley and northern Adirondack Mountains. Our work illustrates a potential pathway for better constraining earthquake-source faults in regions of cryptic faults.
Using RSQSim to Determine Seismic Sequence in Eastern Taiwan Fault System
Authors: Chia-Cheng Hsu , Hung-Yu Wu
Published: September 2024

Understanding the spatial and temporal patterns of seismic activity, along with fault interactions in Taiwan, is essential for earthquake hazard assessment and advancing knowledge of regional tectonics. This study employs the Rate and State Earthquake Simulator (RSQSim) to simulate the eastern Taiwan fault system, integrating fault geometry from the multidisciplinary Taiwan Earthquake Model. We applied long-term simulations spanning 400,000 yr to conduct earthquake sequences and recurrence intervals on five distinct faults in eastern Taiwan: Milun fault, Longitudinal Valley fault, Central Range structure, Luyeh fault, and Taimali Coastline structure. The simulated earthquake catalogs are compared against the historical record in terms of seismicity, frequency–magnitude statistics, and recurrence patterns. The model reasonably reproduces key observational constraints, including spatial patterns, magnitude probabilities fitting a gamma distribution, and periods of quiescence resulting from fault interactions. Overall, the results demonstrate RSQSim’s potential for physics-based seismic hazard modeling and provide insights into regional seismotectonic processes in eastern Taiwan for constructing sustainable cities and communities.
Towards normalizing good practice across the whole modeling cycle: its instrumentation and future research topics
Authors: Jakeman Anthony J. , Elsawah Sondoss , Wang Hsiao-Hsuan , Hamilton Serena H. , Melsen Lieke , Grimm Volker
Published: September 2024

Choices made in modeling matter and demand more explication since they determine how much we can trust modeling insights and predictions within their social, political and ethical contexts. Good Modeling Practice (GMP) is a key research area for strengthening and maturing the modeling field and community, through identifying, formulating and sharing knowledge about the craft of modeling. This craft represents the knowledge that modelers learn in practice about how they get things done, and how they adapt their practices to new situations. This Joint Special Issue is motivated by the importance of sharing good modeling practices from a whole modeling lifecycle viewpoint. We attempt to add conceptual clarity to this research area by defining the plethora of concepts and decision points used to characterize the choices to be made throughout the modeling process, and by synthesizing some of the existing efforts on GMP. We characterize a broad list of articles in the literature on GMP and identify a list of essential topics demanding more attention. This list is only a preliminary one as we anticipate that a more comprehensive list of knowledge gaps will be unearthed from the submissions to the Joint Special Issue collection on GMP, of which this is an introduction. We also propose a vision for GMP and suggest instrumental ways that good practice can become not just well-known but normal practice. This instrumentation focuses on journal standards, collective commitment and culture especially by research community societies, early career awards for advancing GMP, and legal requirements or accreditation. A vital instrument in all this is the design and development of a modeling curriculum that distills core requisite knowledge about modeling, as well as proven-to-work routines and practices that can be scaled up in different contexts.
РОЗРОБКА МЕТОДИКИ КОНТРОЛЮ ТА ДІАГНОСТИКИ ЕКСПЛУАТАЦІЙНИХ ВЛАСТИВОСТЕЙ МАСТИЛЬНИХ МАТЕРІАЛІВ ЗА ТРИБОТЕХНІЧНИМИ ПАРАМЕТРАМИ
Authors: Мнацаканов Рудольф Георгійович , Мікосянчик Оксана Олександрівна , Якобчук Олександр Євгенійович , Хімко Андрій Миколайович , Токарук Віталій Володимирович
Published: September 2024

Запропоновано застосування програмно-апаратного комплексу для оцінки триботехнічних характеристик трибоелементів. Переваги розробленої методики контролю та діагностики експлуатаційних властивостей мастильних матеріалів полягають в неперервній оцінці їх змащувальних, антифрикційних, протизношувальних та реологічних характеристик в нестаціонарних умовах тертя. Апробація методики проведена на оливі «Бора Б» СМ-9.
МЕТОДИКА ДІАГНОСТУВАННЯ РІЗНИХ КОНСТРУКЦІЙ ТРИБОСИСТЕМ МЕТОДОМ АКУСТИЧНОЇ ЕМІСІЇ
Authors: Войтов Віктор Анатолійович , Фененко Катерина Андріївна , Войтов Антон Вікторович
Published: September 2024

У представленій роботі розроблено методику діагностування різних конструкцій трибосистем методом акустичної емісії. В основу розробки методики покладено такі припущення. Активність акустичної емісії в процесі роботи трибосистеми буде залежати від швидкості деформації матеріалів на плямах фактичного контакту. Сумарний акустичний сигнал із зони тертя формується як результат інтерференції первинних акустичних сигналів від плям фактичного контакту з поверхні тертя, які відповідають умові когерентності. Виходячи з даних припущень в роботі представлено вираз для розрахунку інформативних частот, які буде генерувати трибосистема в процесі роботи. Методика діагностування містить наступні етапи: визначення інформативних частот, на яких необхідно виконувати реєстрацію амплітуд; визначення величин амплітуд, які несуть максимальну інформацію; розподіл загального акустичного сигналу на кластери і встановлення функціонального зв'язку значень пік-фактора кластерів з значеннями коефіцієнта тертя, швидкості зношування і часу припрацювання трибосистем в онлайн-режимі. Використання розробленої методики дозволить підвищити робастність методу діагностування трибосистем та ідентифікувати поверхневі процеси під час зношування.
ВПЛИВ ДЖЕРЕЛ НАГРІВУ З ВИКОРИСТАННЯМ ВОДНЕВО-КИСНЕВОЇ СУМІШІ НА ФОРМУВАННЯ СТРУКТУРИ ПРИ НАПЛАВЛЕННІ РЕЛІТНИХ МАТЕРІАЛІВ
Authors: Захаров Євгеній Анатолійович , Попіль Юрій Станіславович , Корж Віктор Миколайович
Published: September 2024

Розглянуті питання по використанню стрічкового реліта для наплавлення на малогабаритні деталі складної форми, з застосуванням в якості джерел енергії, газового полум‘я, плазмового струменя де робочим газом є воднево-киснева суміш. Визначені умови, утворення та формування структури наплавленої поверхні в залежності від теплових характеристик концентрованих джел енергії та технологічних параметрів процесів, представлені результати твердості матриці, характер розподілення твердих часток в ній.