bluuebunny/crossref_metadata_2025 huggingface dataset:
Wild Boars Carry Extended-Spectrum β-Lactamase- and AmpC-Producing Escherichia coli
Authors: Holtmann Anna R. , Meemken Diana , Müller Anja , Seinige Diana , Büttner Kathrin , Failing Klaus , Kehrenberg Corinna
Published: February 2021

Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) represent major healthcare concerns. The role of wildlife in the epidemiology of these bacteria is unclear. The purpose of this study was to determine their prevalence in wild boars in Germany and to characterize individual isolates. A total of 375 fecal samples and 439 nasal swabs were screened for the presence of ESBL-/AmpC-E. coli and MRSA, respectively. The associations of seven demographic and anthropogenic variables with the occurrence of ESBL-/AmpC-E. coli were statistically evaluated. Collected isolates were subjected to antimicrobial susceptibility testing, molecular typing methods, and gene detection by PCR and genome sequencing. ESBL-/AmpC-E. coli were detected in 22 fecal samples (5.9%) whereas no MRSA were detected. The occurrence of ESBL-/AmpC-E. coli in wild boars was significantly and positively associated with human population density. Of the 22 E. coli, 19 were confirmed as ESBL-producers and carried genes belonging to blaCTX-M group 1 or blaSHV-12. The remaining three isolates carried the AmpC-β-lactamase gene blaCMY-2. Several isolates showed additional antimicrobial resistances. All four major phylogenetic groups were represented with group B1 being the most common. This study demonstrates that wild boars can serve as a reservoir for ESBL-/AmpC-producing and multidrug-resistant E. coli.
Editorial for the Special Issue: Clostridium difficile
Authors: Grześkowiak Łukasz M.
Published: February 2021

Clostridium difficile (reclassified as Clostridioides difficile [...]
Contribution of Inhibitory Metabolites and Competition for Nutrients to Colonization Resistance against Clostridioides difficile by Commensal Clostridium
Authors: Reed Amber D. , Theriot Casey M.
Published: February 2021

Clostridioides difficile is an anaerobic pathogen that causes significant morbidity and mortality. Understanding the mechanisms of colonization resistance against C. difficile is important for elucidating the mechanisms by which C. difficile is able to colonize the gut after antibiotics. Commensal Clostridium play a key role in colonization resistance. They are able to modify bile acids which alter the C. difficile life cycle. Commensal Clostridium also produce other inhibitory metabolites including antimicrobials and short chain fatty acids. They also compete with C. difficile for vital nutrients such as proline. Understanding the mechanistic effects that these metabolites have on C. difficile and other gut pathogens is important for the development of new therapeutics against C. difficile infection (CDI), which are urgently needed.
Investigation of Commensal Escherichia coli Populations of Cormorant Hatchlings in the Absence of Anthropogenic Impacts in Remote Areas of West Mongolia
Authors: Khan Muhammad Moman , Kolenda Rafal , Schierack Peter , Weinreich Jörg , Rödiger Stefan , Schierack Jakob , Stubbe Michael , Lkhagvasuren Davaa , Guenther Sebastian , Schaufler Katharina
Published: February 2021

To increase our understanding of bacterial intestinal colonization in animal populations lacking substantial anthropogenic influence we studied the diversity of E. coli in cormorants from the pristine West-Mongolian steppe. E. coli were isolated from individual birds of two cormorant colonies located on small islands in lakes at least 100 km away from human settlements. Diversity of the isolates was studied using pulsed-field gel electrophoresis (PFGE). 137 isolates of cormorant colony-1 and 75 isolates of cormorant colony-2 resulted in 60 and 33 PFGE types, respectively. Representative strains of each PFGE type were analyzed via PCR in terms of phylogroups and extraintestinal virulence-associated genes (exVAGs). Bacterial adhesion to the chicken intestinal cell line CHIC-8E11 and antimicrobial resistance was also determined. Most isolates belonged to phylogroup B1 (68.3%) followed by B2 and E with B2 harboring the highest total number of exVAGs per isolate. Unexpectedly, a PFGE type with relatively few exVAGs displayed the highest isolation frequency, also showing a high adhesion rate. Comparative analysis of exVAGs to other E. coli populations of wildlife origin revealed that the secreted autotransporter toxin encoding sat gene was only present in cormorants. Overall, E. coli in cormorants maintained a high diversity under minimal anthropogenic influences, which likely enables intestinal colonization.
Regulatory Control of Rishirilide(s) Biosynthesis in Streptomyces bottropensis
Authors: Tsypik Olga , Makitrynskyy Roman , Yan Xiaohui , Koch Hans-Georg , Paululat Thomas , Bechthold Andreas
Published: February 2021

Streptomycetes are well-known producers of numerous bioactive secondary metabolites widely used in medicine, agriculture, and veterinary. Usually, their genomes encode 20–30 clusters for the biosynthesis of natural products. Generally, the onset and production of these compounds are tightly coordinated at multiple regulatory levels, including cluster-situated transcriptional factors. Rishirilides are biologically active type II polyketides produced by Streptomyces bottropensis. The complex regulation of rishirilides biosynthesis includes the interplay of four regulatory proteins encoded by the rsl-gene cluster: three SARP family regulators (RslR1-R3) and one MarR-type transcriptional factor (RslR4). In this work, employing gene deletion and overexpression experiments we revealed RslR1-R3 to be positive regulators of the biosynthetic pathway. Additionally, transcriptional analysis indicated that rslR2 is regulated by RslR1 and RslR3. Furthermore, RslR3 directly activates the transcription of rslR2, which stems from binding of RslR3 to the rslR2 promoter. Genetic and biochemical analyses demonstrated that RslR4 represses the transcription of the MFS transporter rslT4 and of its own gene. Moreover, DNA-binding affinity of RslR4 is strictly controlled by specific interaction with rishirilides and some of their biosynthetic precursors. Altogether, our findings revealed the intricate regulatory network of teamworking cluster-situated regulators governing the biosynthesis of rishirilides and strain self-immunity.
Ultrasound-Assisted Extraction of Anthocyanins Using Natural Deep Eutectic Solvents and Their Incorporation in Edible Films
Authors: Velásquez Patricia , Bustos Daniela , Montenegro Gloria , Giordano Ady
Published: February 2021

Extracts rich in bioactive compounds added to edible films have allowed the development of active packaging that increases the shelf life of food. However, it is necessary to search for solvents that are nontoxic and not harmful to the environment, with natural deep eutectic solvents (NADES) being an attractive and easily synthesized alternative. This research aimed to design NADES by lyophilization to be used in the extraction of anthocyanins from the Chilean Luma chequen (Molina) A. Gray berry, and subsequently adding them to the matrix of edible ƙ-carrageenan films. For this purpose, ultrasound-assisted extraction (UAE) was used and the anthocyanin content was evaluated with the pH differential method. The antioxidant capacity of extracts was determined by DPPH assay and the antibacterial capacity by diffusion agar tests. The results obtained indicate that the designed NADES are efficient at extracting anthocyanins, reaching concentrations between 81.1 and 327.6 mg eq cyanidin 3-glucoside/100 g dw of L. chequen (Molina) A. Gray. The extracts reached inhibition diameters between 5 and 34 mm against Escherichia coli, Staphylococcus aureus, and Salmonella typhi strains. Once the extracts were incorporated into ƙ-carrageenan films, active edible films with antioxidant and antibacterial capacities were obtained.
Antioxidant and Anti-Inflammatory Potential of Polyphenols Contained in Mediterranean Diet in Obesity: Molecular Mechanisms
Authors: Nani Abdelhafid , Murtaza Babar , Sayed Khan Amira , Khan Naim Akhtar , Hichami Aziz
Published: February 2021

Nutrition transition can be defined as shifts in food habits, and it is characterized by high-fat (chiefly saturated animal fat), hypercaloric and salty food consumption at the expense of dietary fibers, minerals and vitamins. Western dietary patterns serve as a model for studying the impact of nutrition transition on civilization diseases, such as obesity, which is commonly associated with oxidative stress and inflammation. In fact, reactive oxygen species (ROS) overproduction can be associated with nuclear factor-κB (NF-κB)-mediated inflammation in obesity. NF-κB regulates gene expression of several oxidant-responsive adipokines including tumor necrosis factor-α (TNF-α). Moreover, AMP-activated protein kinase (AMPK), which plays a pivotal role in energy homeostasis and in modulation of metabolic inflammation, can be downregulated by IκB kinase (IKK)-dependent TNF-α activation. On the other hand, adherence to a Mediterranean-style diet is highly encouraged because of its healthy dietary pattern, which includes antioxidant nutraceuticals such as polyphenols. Indeed, hydroxycinnamic derivatives, quercetin, resveratrol, oleuropein and hydroxytyrosol, which are well known for their antioxidant and anti-inflammatory activities, exert anti-obesity proprieties. In this review, we highlight the impact of the most common polyphenols from Mediterranean foods on molecular mechanisms that mediate obesity-related oxidative stress and inflammation. Hence, we discuss the effects of these polyphenols on a number of signaling pathways. We note that Mediterranean diet (MedDiet) dietary polyphenols can de-regulate nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) and NF-κB-mediated oxidative stress, and metabolic inflammation. MedDiet polyphenols are also effective in upregulating downstream effectors of several proteins, chiefly AMPK.
Oral Sucrosomial Iron Is as Effective as Intravenous Ferric Carboxy-Maltose in Treating Anemia in Patients with Ulcerative Colitis
Authors: Bertani Lorenzo , Tricò Domenico , Zanzi Federico , Baiano Svizzero Giovanni , Coppini Francesca , de Bortoli Nicola , Bellini Massimo , Antonioli Luca , Blandizzi Corrado , Marchi Santino
Published: February 2021

Anemia is a frequent complication of ulcerative colitis, and is frequently caused by iron deficiency. Oral iron supplementation displays high rates of gastrointestinal adverse effects. However, the formulation of sucrosomial iron (SI) has shown higher tolerability. We performed a prospective study to compare the effectiveness and tolerability of oral SI and intravenous ferric carboxy-maltose (FCM) in patients with ulcerative colitis in remission and mild-to-moderate anemia. Patients were randomized 1:1 to receive 60 mg/day for 8 weeks and then 30 mg/day for 4 weeks of oral SI or intravenous 1000 mg of FCM at baseline. Hemoglobin and serum levels of iron and ferritin were assessed after 4, 8, and 12 weeks from baseline. Hemoglobin and serum iron increased in both groups after 4 weeks of therapy, and remained stable during follow up, without significant treatment or treatment-by-time interactions (p = 0.25 and p = 0.46 for hemoglobin, respectively; p = 0.25 and p = 0.26 for iron, respectively). Serum ferritin did not increase over time during SI supplementation, while it increased in patients treated with FCM (treatment effect, p = 0.0004; treatment-by-time interaction effect, p = 0.0002). Overall, this study showed that SI and FCM displayed similar effectiveness and tolerability for treatment of mild-to-moderate anemia in patients with ulcerative colitis under remission.
The Price of Coastal Erosion and Flood Risk: A Hedonic Pricing Approach
Authors: Catma Serkan
Published: February 2021

Accelerated coastal erosion and elevated risks of flooding due to global warming put enormous burden on the ecosystems and economic health of coastal communities. Optimal policies to lessen these negative impacts require an estimation of their costs and benefits. The aim of this paper is to calculate the costs of beach erosion and flood risk through the valuation of property prices in Hilton Head Island, a barrier island located in South Carolina, USA. Spatial lag hedonic pricing was introduced in order to account for spatial autocorrelation in the dataset. The results show that properties that are located within the zone of high, or very high, flood risk experience a 15.6% reduction in value. The implicit price of being located close to an eroded beach is approximately 26% of the price of an oceanfront property. However, this negative impact on property value diminishes with distance from the shoreline.
Canopy Accumulation: Are Seagrass Meadows a Sink of Microplastics?
Authors: Unsworth Richard K. F. , Higgs Alex , Walter Bettina , Cullen-Unsworth Leanne C. , Inman Isabella , Jones Benjamin L.
Published: February 2021

A growing body of research is documenting the accumulation of microplastics within marine sediments around the world. The hydrodynamic influences of seagrasses in coastal environments are shown to increase sedimentation of finer particles and as a result there has been speculation that this attribute will lead to seagrass meadows acting as a site of elevated microplastic contamination. To date a range of localised studies have provided conflicting answers to this hypothesis. Seagrass meadows provide multiple ecosystem services including vital support roles for a range of fisheries; therefore, there are considerable human health implications for understanding their role as sinks of microplastics. This research investigated the abundance and diversity of microplastics present in temperate North Atlantic seagrass meadow sediments relative to unvegetated sediments and examined how they correlate with the meadow structure and the sediment type. We also placed this data in the context of the current knowledge of microplastics in seagrass sediments through a global meta-analysis of published data. Eight seagrass meadows and adjacent unvegetated sites around the UK were sampled to test for the abundance of microplastic particles in the sediment. Microplastics were found in 98% of the samples, with fibres making up 91.8% of all microplastics identified. Abundance was recorded to overall be 215 ± 163 microplastic particles (MP) kg−1 Dry Weight (DW) of sediment in seagrass and 221 ± 236 MP kg−1 DW of sediment in unvegetated habitats. There were no significant differences found between the number of MP with respect to vegetation. We report evidence of the almost ubiquitous contamination of seagrass sediments with microplastics both in the UK and globally but find that the contamination reflects a general build-up of microplastics in the wider environment rather than becoming concentrated within seagrass as an enhanced sink. Microplastic build up in sediments is hypothesised to be the result of local hydrodynamics and plastic sources rather than the result of elevated habitat level concentration. Although not of a higher abundance in seagrass, such contamination in seagrass is of cause for concern given the high dependency of many species of fish on these habitat types and the potential for plastics to move up the food chain.
Directed Transport of CRP Across In Vitro Models of the Blood-Saliva Barrier Strengthens the Feasibility of Salivary CRP as Biomarker for Neonatal Sepsis
Authors: Lin Grace C. , Küng Erik , Smajlhodzic Merima , Domazet Sandra , Friedl Heinz P. , Angerer Joachim , Wisgrill Lukas , Berger Angelika , Bingle Lynne , Peham Johannes R. , Neuhaus Winfried
Published: February 2021

C-reactive protein (CRP) is a commonly used serum biomarker for detecting sepsis in neonates. After the onset of sepsis, serial measurements are necessary to monitor disease progression; therefore, a non-invasive detection method is beneficial for neonatal well-being. While some studies have shown a correlation between serum and salivary CRP levels in septic neonates, the causal link behind this correlation remains unclear. To investigate this relationship, CRP was examined in serum and saliva samples from 18 septic neonates and compared with saliva samples from 22 healthy neonates. While the measured blood and saliva concentrations of the septic neonates varied individually, a correlation of CRP levels between serum and saliva samples was observed over time. To clarify the presence of active transport of CRP across the blood–salivary barrier (BSB), transport studies were performed with CRP using in vitro models of oral mucosa and submandibular salivary gland epithelium. The results showed enhanced transport toward saliva in both models, supporting the clinical relevance for salivary CRP as a biomarker. Furthermore, CRP regulated the expression of the receptor for advanced glycation end products (RAGE) and the addition of soluble RAGE during the transport studies indicated a RAGE-dependent transport process for CRP from blood to saliva.
A Sociotechnical Approach to Analyze Pharmaceutical Policy and Services Management in Primary Health Care in a Brazilian Municipality
Authors: da Bernardo Noemia Liege Maria , Soares Luciano , Leite Silvana Nair
Published: February 2021

The decentralization of the Brazilian health system required that municipalities took responsibility for the local Pharmaceutical Policy and Services (PPS) system. This article presents and analyses an innovative experience of diagnosis of municipal PPS as a sociotechnical system. We adopted a multi-methods approach and various data sources. Sociotechnical theory was the framework of the methodology of evaluation and design of systems, analyzing the External System (health system, stakeholders, financing) and Internal System (goals, management, workforce, infrastructure, processes, technology and culture). The “objective” component of the PPS system was identified as the central element. The lack of a unified objective and of a central coordination and unmanaged pharmaceutical services prevented integrated internal planning and planning with other sectors. Stakeholders and documents referred only to technical elements of the system: Infrastructure, technical process, and technology. The social components of the workforce and culture were not mentioned. The organizational culture established was the culture of isolation: “Each one does his own”. The pharmacists working in the municipal health system did not know each other. There was no integration strategy between pharmacists and their work processes. Consequently, the municipal PPS had limited scope as a public policy. It had constrained the characteristics of PPS as a complex and open system. Understanding the municipal PPS as a sociotechnical system can push the development of a new level of policy and practice to ensure the population’s right to the access to and rational use of medicines.
Relativistic Ermakov–Milne–Pinney Systems and First Integrals
Authors: Haas Fernando
Published: February 2021

The Ermakov–Milne–Pinney equation is ubiquitous in many areas of physics that have an explicit time-dependence, including quantum systems with time-dependent Hamiltonian, cosmology, time-dependent harmonic oscillators, accelerator dynamics, etc. The Eliezer and Gray physical interpretation of the Ermakov–Lewis invariant is applied as a guiding principle for the derivation of the special relativistic analog of the Ermakov–Milne–Pinney equation and associated first integral. The special relativistic extension of the Ray–Reid system and invariant is obtained. General properties of the relativistic Ermakov–Milne–Pinney are analyzed. The conservative case of the relativistic Ermakov–Milne–Pinney equation is described in terms of a pseudo-potential, reducing the problem to an effective Newtonian form. The non-relativistic limit is considered to be well. A relativistic nonlinear superposition law for relativistic Ermakov systems is identified. The generalized Ermakov–Milne–Pinney equation has additional nonlinearities, due to the relativistic effects.
Exogenously Used 24-Epibrassinolide Promotes Drought Tolerance in Maize Hybrids by Improving Plant and Water Productivity in an Arid Environment
Authors: Desoky El-Sayed M. , Mansour Elsayed , Ali Mohamed M. A. , Yasin Mohamed A. T. , Abdul-Hamid Mohamed I. E. , Rady Mostafa M. , Ali Esmat F.
Published: February 2021

The influence of 24-epibrassinolide (EBR24), applied to leaves at a concentration of 5 μM, on plant physio-biochemistry and its reflection on crop water productivity (CWP) and other agronomic traits of six maize hybrids was field-evaluated under semi-arid conditions. Two levels of irrigation water deficiency (IWD) (moderate and severe droughts; 6000 and 3000 m3 water ha−1, respectively) were applied versus a control (well-watering; 9000 m3 water ha−1). IWD reduced the relative water content, membrane stability index, photosynthetic efficiency, stomatal conductance, and rates of transpiration and net photosynthesis. Conversely, antioxidant enzyme activities and osmolyte contents were significantly increased as a result of the increased malondialdehyde content and electrolyte leakage compared to the control. These negative influences of IWD led to a reduction in CWP and grain yield-related traits. However, EBR24 detoxified the IWD stress effects and enhanced all the above-mentioned parameters. The evaluated hybrids varied in drought tolerance; Giza-168 was the best under moderate drought, while Fine-276 was the best under severe drought. Under IWD, certain physiological traits exhibited a highly positive association with yield and yield-contributing traits or CWP. Thus, exogenously using EBR24 for these hybrids could be an effective approach to improve plant and water productivity under reduced available water in semi-arid environments.
Molecular Imprinting Strategies for Tissue Engineering Applications: A Review
Authors: Bonatti Amedeo Franco , De Maria Carmelo , Vozzi Giovanni
Published: February 2021

Tissue Engineering (TE) represents a promising solution to fabricate engineered constructs able to restore tissue damage after implantation. In the classic TE approach, biomaterials are used alongside growth factors to create a scaffolding structure that supports cells during the construct maturation. A current challenge in TE is the creation of engineered constructs able to mimic the complex microenvironment found in the natural tissue, so as to promote and guide cell migration, proliferation, and differentiation. In this context, the introduction inside the scaffold of molecularly imprinted polymers (MIPs)—synthetic receptors able to reversibly bind to biomolecules—holds great promise to enhance the scaffold-cell interaction. In this review, we analyze the main strategies that have been used for MIP design and fabrication with a particular focus on biomedical research. Furthermore, to highlight the potential of MIPs for scaffold-based TE, we present recent examples on how MIPs have been used in TE to introduce biophysical cues as well as for drug delivery and sequestering.
Classification and Analysis of Optimization Techniques for Integrated Energy Systems Utilizing Renewable Energy Sources: A Review for CHP and CCHP Systems
Authors: Bagherian Mohammad Ali , Mehranzamir Kamyar , Pour Amin Beiranvand , Rezania Shahabaldin , Taghavi Elham , Nabipour-Afrouzi Hadi , Dalvi-Esfahani Mohammad , Alizadeh Seyed Morteza
Published: February 2021

Energy generation and its utilization is bound to increase in the following years resulting in accelerating depletion of fossil fuels, and consequently, undeniable damages to our environment. Over the past decade, despite significant efforts in renewable energy realization and developments for electricity generation, carbon dioxide emissions have been increasing rapidly. This is due to the fact that there is a need to go beyond the power sector and target energy generation in an integrated manner. In this regard, energy systems integration is a concept that looks into how different energy systems, or forms, can connect together in order to provide value for consumers and producers. Cogeneration and trigeneration are the two most well established technologies that are capable of producing two or three different forms of energy simultaneously within a single system. Integrated energy systems make for a very strong proposition since it results in energy saving, fuel diversification, and supply of cleaner energy. Optimization of such systems can be carried out using several techniques with regards to different objective functions. In this study, a variety of optimization methods that provides the possibility of performance improvements, with or without presence of constraints, are demonstrated, pinpointing the characteristics of each method along with detailed statistical reports. In this context, optimization techniques are classified into two primary groups including unconstrained optimization and constrained optimization techniques. Further, the potential applications of evolutionary computing in optimization of Integrated Energy Systems (IESs), particularly Combined Heat and Power (CHP) and Combined Cooling, Heating, and Power (CCHP), utilizing renewable energy sources are grasped and reviewed thoroughly. It was illustrated that the employment of classical optimization methods is fading out, replacing with evolutionary computing techniques. Amongst modern heuristic algorithms, each method has contributed more to a certain application; while the Genetic Algorithm (GA) was favored for thermoeconomic optimization, Particle Swarm Optimization (PSO) was mostly applied for economic improvements. Given the mathematical nature and constraint satisfaction property of Mixed-Integer Linear Programming (MILP), this method is gaining prominence for scheduling applications in energy systems.
Cyanidin-3-glucoside Lipophilic Conjugates for Topical Application: Tuning the Antimicrobial Activities with Fatty Acid Chain Length
Authors: Oliveira Hélder , Correia Patrícia , Bessa Lucinda J. , Guimarães Marta , Gameiro Paula , Freitas Victor de , Mateus Nuno , Cruz Luís , Fernandes Iva
Published: February 2021

Background: Natural anthocyanins present a low solubility in lipophilic media, which compromises their effective application in lipophilic systems. In this work, cyanidin-3-O-glucoside (Cy3glc) was esterified by the addition of fatty acids with increasing chain-lengths and a structure-activity relationship was performed towards the description of the best analog for skin-care applications. Methods: By enzymatic hemi-synthesis, it was possible to obtain 5 structurally related derivatives of cyanidin-3-O-glucoside with successive C2 increments in the aliphatic chain. The stability in hanks buffer and DMEM with or without FBS was followed by HPLC. The cytotoxicity against keratinocytes was evaluated by MTT assay. The antioxidant capacity was determined by using the fluorescent probe DCF-DA. The effect on enzyme activity was evaluated towards tyrosinase, collagenase, and elastase enzymes by colorimetric assays. MIC and MBC values were obtained against reference strains and against multidrug-resistant isolates. Results: In physiological conditions, cy3glc−fatty acid derivatives are more stable and may be converted to the native anthocyanin. The 5 conjugates showed lower antioxidant capacity and enzymatic inhibitory activities in comparison to the anthocyanin precursor. However, concerning the antibacterial activity, the insertion of a fatty acid chain sprouted the antibacterial activity, showing a clear biphasic effect and a more effective effect on Gram-positive bacteria. Conclusions: Cy3glc-C10 was the most effective compound considering the antimicrobial activity, although a general reduction was observed among the other activities evaluated. This work prompt further assays with a different panoply of derivatives ranging other features including saturation vs. unsaturation, even vs. odd carbon content and linear vs. branched.
Crustal Deformation Across and beyond Central Europe and Its Impact on Land Boundaries
Authors: Maciuk Kamil , Peska-Siwik Agnieszka , El-Mowafy Ahmed , Borowski Lukasz , Apollo Michal
Published: February 2021

Land is a critical and limited natural resource. The Land Administration System (LAS) has been developed to resolve and adjudicate over any disputes that might arise concerning the rights and boundaries of land. Land registration and cadastre are types of land recording that need to be established. To secure the property rights, we must be sure of accuracy of the boundary points determining the size of the property. However, in addition to typical factors considered when determining the boundary point positions, such as accuracy of geodetic networks and measurement errors, the global and local crustal deformation, resulting, e.g., from the movement of tectonic plates, should be considered. In this work, the focus is on the movement of points inside the European plate due to tectonic movement, without taking into account local events caused by erosion, landslides, etc. The study area is Europe, and particular attention was paid to Poland, which is located in the centre of the European continent and does not have significant anomalous sub-areas, making it an authoritative research object. In this study, we analysed the velocity of point displacements and the boundary deformation, using GPS observations. For this reason, we used both global (IGS) and regional (ETRF) reference frames, to show differences in point velocities for the studied areas. Overall, for the needs of the real estate cadastre in Poland, information about parcel boundary points must be obtained with an accuracy better than 0.30 m. Within 25 years, the border mark may be shifted by 0.13 m due to tectonic plate movement, which is within the required accuracy. Pursuant to the current legal regulations, the measurements of the boundary points can be performed with any method, ensuring the required accuracy (0.30 m). The most commonly used are direct measurements (GNSS and tacheometry) and photogrammetric measurements. It is recommended that periodic verifications and update of the cadastre data in Poland be carried out at least once every 15 years. In the case of such relatively frequent verification and possible modernisation of data, the potential impact of tectonic plate movement on the relative boundary point displacement can be ignored, particularly in the short term. However, for a long time period it has an influence. We suggest “relatively frequent” cadastral boundary verification to be able to ignore such influence.
Instruments of Mineral Deposit Safeguarding in Poland, Slovakia and Czechia—Comparative Analysis
Authors: Gałaś Slávka , Kot-Niewiadomska Alicja , Gałaś Andrzej , Kondela Julián , Wertichová Blažena
Published: February 2021

Mineral deposits are essential for the economic, technological and social development. However, to enable them to play an appropriate role in the process of sustainable development, they need to be safeguarded in a comprehensive and systemic manner in the same measure as other elements of the environment. The practice of securing access to areas where the mineral deposits can be found is based on the statement that they can be extracted only in places where they occur. This fact defines the type and scope of instruments for safeguarding prospective deposit areas of minerals and their documented deposits. These issues gained in significance in the EU level in recent years however views on this subject across the Member States still vary. The paper subjects instruments of mineral deposit safeguarding used in Poland, Slovakia and Czechia to the analysis and multi-criteria comparative assessment. It recommends their division into the conceptual, legal, spatial planning and economic ones. As a result of studies, similarities and differences in the approach to mineral deposit safeguarding in individual countries are shown, indicating good practices and suggesting possible changes. The analysis revealed many analogies in actions aimed at mineral deposit safeguarding in individual countries, however the assessment of their effectiveness and implementation points at the necessity of taking further steps to increase deposit safeguarding.
Spatiotemporal Evolution of Multiscale Urbanization Level in the Beijing-Tianjin-Hebei Region Using the Integration of DMSP/OLS and NPP/VIIRS Night Light Datasets
Authors: Wang Jingtao , Liu Haibin , Liu Hao , Huang Hui
Published: February 2021

The level of urbanization is a key factor in urban development. In this study, to better characterize the level of urbanization, the panel entropy weight method is used to weight the factors of population, industry, and area to construct a composite indicator of urbanization. A panel regression between this composite index and the average night light values after fusion shows a strong correlation. An accuracy test indicates that the estimated value of fused average light as calculated by the urbanization level estimation model that adequately represents the urbanization level. On this basis, night light data is corrected for zero error on the pixel scale, and spatiotemporal evolution analyses are performed on the city and county scales. The standard deviation ellipse method is used to find that the spatial distribution pattern of the Beijing-Tianjin-Hebei urbanization level from 1995 to 2018 radiates and spreads to the northeast, with Beijing-Tianjin as the center. The spatial pattern shows a contracting trend that is strengthening year by year. Slope analyses show that areas with rapid urbanization growth are mainly concentrated in Beijing and Tianjin. The urbanization development speed of most counties in the Hebei Province is at a low level.
Does Economic Agglomeration Lead to Efficient Rural to Urban Land Conversion? An Examination of China’s Metropolitan Area Development Strategy
Authors: Huang Ke , Dallimer Martin , Stringer Lindsay C. , Zhang Anlu , Zhang Ting
Published: February 2021

Urbanization involves expansion of the amount of land covered by urban uses. Rural to urban land conversion (RULC) can satisfy demand for the additional space that growing cities require. However, there can be negative consequences, such as the loss of productive agricultural land and/or the destruction of natural habitats. Considerable interest therefore exists among policy makers and researchers regarding how the efficiency of RULC can be maximized. We used the Gini index and a data envelopment analysis to quantify the relationship between RULC and economic development for 17 metropolitan areas in China. We did this from two perspectives: (i) coordination; and (ii) efficiency. We found that economic agglomeration fosters the coordination of the amount of rural land that is allocated to be converted to urban uses. Similarly, economic agglomeration increases the efficiency of RULC in terms of the processes of socio-economic production. Through production technology innovation and readjustment in the scale of input factors, the productive efficiency of RULC can be promoted. Our findings suggest a need to strictly limit the amount of RULC, design differential land management policies according to location and development level, and adjust RULC allocation between different cities. Further, in harnessing the potential of intensive urban land use and restructuring, production factors, including land, can be enhanced through technological innovation. Research presented in this paper provides insights for areas of the world which are yet to undergo the rapid urbanization that China has experienced, but where it is projected to occur over the coming decades.
Unobtrusive Continuous Stress Detection in Knowledge Work—Statistical Analysis on User Acceptance
Authors: Kallio Johanna , Vildjiounaite Elena , Kantorovitch Julia , Kinnula Atte , Bordallo López Miguel
Published: February 2021

Modern knowledge work is highly intense and demanding, exposing workers to long-term psychosocial stress. In order to address the problem, stress detection technologies have been developed, enabling the continuous assessment of personal stress based on multimodal sensor data. However, stakeholders lack insights into how employees perceive different monitoring technologies and whether they are willing to share stress-indicative data in order to sustain well-being at the individual, team, and organizational levels in the knowledge work context. To fill this research gap, we developed a theoretical model for knowledge workers’ interest in sharing their stress-indicative data collected with unobtrusive sensors and examined it empirically using structural equation modeling (SEM) with a survey of 181 European knowledge workers. The results did not show statistically significant privacy concerns regarding environmental sensors such as air quality, sound level, and motion sensors. On the other hand, concerns about more privacy-sensitive methods such as tracking personal device usage patterns did not prevent user acceptance nor intent to share data. Overall, knowledge workers were highly interested in employing stress monitoring technologies to measure their stress levels and receive information about their personal well-being. The results validate the willingness to accept the unobtrusive, continuous stress detection in the context of knowledge work.
Spatiotemporal Dynamics of Nitrogen Budgets under Anthropogenic Activities in Metropolitan Areas
Authors: Ding Ning , Zhu Jingfeng , Li Xiao , Wang Xiangrong
Published: February 2021

The rapid growth of metropolitan regions is closely associated with high nitrogen (N) flows, which is known as the most important reason for widespread water pollution. It is, therefore, crucial to explore the spatiotemporal patterns of N budgets under intensive human activity. In this study, we estimated the long-term (2000–2015) N budgets by integrating the net anthropogenic nitrogen input (NANI) and the export coefficient model (ECM) in the Yangtze River Delta Urban Agglomeration (YRDUA), a typical metropolitan area with strong human disturbances. The results revealed that the NANI decreased by 10% from 2000 to 2015, while N exports showed a 6% increase. Hotspots for N budgets were found in the northeastern areas, where cropland and construction land were dominant. The linear regression showed a close relationship between the NANI and N export, and about 18% of the NANI was exported into the river system. By revealing the critical sources and drivers of N budgets over time, our work aimed to provide effective information for regional policy on nitrogen management. Future strategies, such as improving the fertilizer efficiency, optimizing the land use pattern, and controlling the population density, are necessary in order to address the environmental challenge concerns of excessive N.
The Potential of Plastic Reuse for Manufacturing: A Case Study into Circular Business Models for an On-Line Marketplace
Authors: de Jong Annelise M. , Mellquist Ann-Charlotte
Published: February 2021

The plastic industry is facing increasingly growing social and political demands on plastic recycling and reuse. The resource perspective is essential for plastic production companies. Circular economy is one perspective for how industries could deal with a lack of resources today and in the future. However, there are large challenges in the reuse of plastics waste, predominantly due to concern regarding the quality of the waste material. Our paper focuses on the potential of the exchange of plastic material waste between companies in the production process before it enters into the recycling loop. In this study our aim is to investigate if an on-line marketplace is relevant for the plastic industry for this exchange, the market potential and the potential business model and requirements of the marketplace. Through interviews and benchmarking with five platform providers and two plastic manufacturing companies, we collected information on the business models of international digital platforms and plastic manufacturers concerning the exchange of reusable materials between companies. We also collected data through a survey sent out to plastic manufacturers via two industry organizations in Sweden. This paper will present the results from the study and conclude with a description of draft requirements with particular focus on direct reuse of regrind material, and a potential business model for the on-line marketplace that should be run on a commercial basis. This study shows that there are opportunities for the plastic industry to be at the forefront of a circular initiative that could also be utilized by other industries with adjustments to reflect the specific requirements of each industry.
DroidbotX: Test Case Generation Tool for Android Applications Using Q-Learning
Authors: Yasin Husam N. , Hamid Siti Hafizah Ab , Raja Yusof Raja Jamilah
Published: February 2021

Android applications provide benefits to mobile phone users by offering operative functionalities and interactive user interfaces. However, application crashes give users an unsatisfactory experience, and negatively impact the application’s overall rating. Android application crashes can be avoided through intensive and extensive testing. In the related literature, the graphical user interface (GUI) test generation tools focus on generating tests and exploring application functions using different approaches. Such tools must choose not only which user interface element to interact with, but also which type of action to be performed, in order to increase the percentage of code coverage and to detect faults with a limited time budget. However, a common limitation in the tools is the low code coverage because of their inability to find the right combination of actions that can drive the application into new and important states. A Q-Learning-based test coverage approach developed in DroidbotX was proposed to generate GUI test cases for Android applications to maximize instruction coverage, method coverage, and activity coverage. The overall performance of the proposed solution was compared to five state-of-the-art test generation tools on 30 Android applications. The DroidbotX test coverage approach achieved 51.5% accuracy for instruction coverage, 57% for method coverage, and 86.5% for activity coverage. It triggered 18 crashes within the time limit and shortest event sequence length compared to the other tools. The results demonstrated that the adaptation of Q-Learning with upper confidence bound (UCB) exploration outperforms other existing state-of-the-art solutions.
Shiga Toxin (Stx)-Binding Glycosphingolipids of Primary Human Renal Cortical Epithelial Cells (pHRCEpiCs) and Stx-Mediated Cytotoxicity
Authors: Detzner Johanna , Krojnewski Elisabeth , Pohlentz Gottfried , Steil Daniel , Humpf Hans-Ulrich , Mellmann Alexander , Karch Helge , Müthing Johannes
Published: February 2021

Human kidney epithelial cells are supposed to be directly involved in the pathogenesis of the hemolytic–uremic syndrome (HUS) caused by Shiga toxin (Stx)-producing enterohemorrhagic Escherichia coli (EHEC). The characterization of the major and minor Stx-binding glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer), respectively, of primary human renal cortical epithelial cells (pHRCEpiCs) revealed GSLs with Cer (d18:1, C16:0), Cer (d18:1, C22:0), and Cer (d18:1, C24:1/C24:0) as the dominant lipoforms. Using detergent-resistant membranes (DRMs) and non-DRMs, Gb3Cer and Gb4Cer prevailed in the DRM fractions, suggesting their association with microdomains in the liquid-ordered membrane phase. A preference of Gb3Cer and Gb4Cer endowed with C24:0 fatty acid accompanied by minor monounsaturated C24:1-harboring counterparts was observed in DRMs, whereas the C24:1 fatty acid increased in relation to the saturated equivalents in non-DRMs. A shift of the dominant phospholipid phosphatidylcholine with saturated fatty acids in the DRM to unsaturated species in the non-DRM fractions correlated with the GSL distribution. Cytotoxicity assays gave a moderate susceptibility of pHRCEpiCs to the Stx1a and Stx2a subtypes when compared to highly sensitive Vero-B4 cells. The results indicate that presence of Stx-binding GSLs per se and preferred occurrence in microdomains do not necessarily lead to a high cellular susceptibility towards Stx.
An Oncolytic Adenovirus Encoding SA-4-1BBL Adjuvant Fused to HPV-16 E7 Antigen Produces a Specific Antitumor Effect in a Cancer Mouse Model
Authors: Martinez-Perez Alejandra G. , Perez-Trujillo Jose J. , Garza-Morales Rodolfo , Ramirez-Avila Norma E. , Loera-Arias Maria J. , Gomez-Gutierrez Jorge G. , Saucedo-Cardenas Odila , Garcia-Garcia Aracely , Rodriguez-Rocha Humberto , Montes-de-Oca-Luna Roberto
Published: February 2021

Human papillomaviruses (HPVs) are responsible for about 25% of cancer cases worldwide. HPV-16 E7 antigen is a tumor-associated antigen (TAA) commonly expressed in HPV-induced tumors; however, it has low immunogenicity. The interaction of 4-1BBL with its receptor induces pleiotropic effects on innate, adaptive, and regulatory immunity and, if fused to TAAs in DNA vaccines, can improve the antitumor response; however, there is low transfection and antitumor efficiency. Oncolytic virotherapy is promising for antitumor gene therapy as it can be selectively replicated in tumor cells, inducing cell lysis, and furthermore, tumor cell debris can be taken in by immune cells to potentiate antitumor responses. In this study, we expressed the immunomodulatory molecule SA-4-1BBL fused to E7 on an oncolytic adenovirus (OAd) system. In vitro infection of TC-1 tumor cells and NIH-3T3 non-tumor cells with SA/E7/4-1BBL OAd demonstrated that only tumor cells are selectively destroyed. Moreover, protein expression is targeted to the endoplasmic reticulum in both cell lines when a signal peptide (SP) is added. Finally, in an HPV-induced cancer murine model, the therapeutic oncolytic activity of OAd can be detected, and this can be improved when fused to E7 and SP.
Life span, dispersal and age of nesting Great Grey Owls Strix nebulosa lapponica in Sweden
Authors: Solheim Roar , Stefansson Ove
Published: February 2021

3,073 Great Grey Owls were banded in Sweden in 1955–2012. 416 were controlled at least once (54.6%) or recovered dead (45.4%). Three birds banded as nestlings were recovered in their 17th calendar year. Most birds were recovered during first year of life. Only 4 females were controlled breeding as 2CY birds. 91.3% of birds controlled as first time breeders were at least 4CY. Birds banded as nestlings and recovered dead between September and July moved 100.8 km (mean) with a median distance of 64 km. Juveniles controlled alive moved 45.9 km (mean) with a median distance of 23 km during first year of life. Maximum natal dispersal was 650 km. Median natal dispersal for females was 40 km, between 7 km and 115 km depending on longevity from hatching to control. Females at ages 2CY, 5CY, 8CY and 11CY had the lowest median natal dispersals. Median secondary dispersal distances (from one nest to a later nestsite) for females lay between 0 km and 9 km. For males both mean and median secondary dispersal distances were less than 3 km.