bluuebunny/crossref_metadata_2025 huggingface dataset:
Adult-Onset CNS Sulfatide Deficiency Causes Sex-Dependent Metabolic Disruption in Aging
Authors: Qiu Shulan , He Sijia , Wang Jianing , Wang Hu , Bhattacharjee Anindita , Li Xin , Saeed Moawiz , Dupree Jeffrey L. , Han Xianlin
Published: June 2023

The interconnection between obesity and central nervous system (CNS) neurological dysfunction has been widely appreciated. Accumulating evidence demonstrates that obesity is a risk factor for CNS neuroinflammation and cognitive impairment. However, the extent to which CNS disruption influences peripheral metabolism remains to be elucidated. We previously reported that myelin-enriched sulfatide loss leads to CNS neuroinflammation and cognitive decline. In this study, we further investigated the impact of CNS sulfatide deficiency on peripheral metabolism while considering sex- and age-specific effects. We found that female sulfatide-deficient mice gained significantly more body weight, exhibited higher basal glucose levels, and were glucose-intolerant during glucose-tolerance test (GTT) compared to age-matched controls under a normal diet, whereas male sulfatide-deficient mice only displayed glucose intolerance at a much older age compared to female sulfatide-deficient mice. Mechanistically, we found that increased body weight was associated with increased food intake and elevated neuroinflammation, especially in the hypothalamus, in a sex-specific manner. Our results suggest that CNS sulfatide deficiency leads to sex-specific alterations in energy homeostasis via dysregulated hypothalamic control of food intake.
A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions
Authors: Ferrà-Cañellas Maria del Mar , Munar-Bestard Marta , Floris Ilaria , Ramis Joana Maria , Monjo Marta , Garcia-Sureda Laura
Published: June 2023

Periodontal therapies use immune mediators, but their side effects can increase with dosage. Micro-immunotherapy (MI) is a promising alternative that employs immune regulators at low and ultralow doses to minimize adverse effects. In this study, the effects of 5 capsules and the entire 10-capsule sequence of the sequential MI medicine (MIM-seq) were tested in two in vitro models of periodontitis. Firstly, human gingival fibroblasts (hGFs) exposed to interleukin (IL)-1β to induce inflammation were treated with five different capsules of MIM-seq for 3 days or with MIM-seq for 24 days. Subsequently, MIM-seq was analyzed in a 3D model of human tissue equivalent of gingiva (GTE) under the same inflammatory stimulus. Simultaneously, a non-IL-1β-treated control and a vehicle were included. The effects of the treatments on cytotoxicity, collagen deposition, and the secreted levels of IL-1α, IL-6, prostaglandin E2 (PGE2), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were evaluated. None of the tested items were cytotoxic. The complete sequence of MIM-seq decreased PGE2 release and restored collagen deposition levels induced by IL-1β treatment in hGFs exposed to IL-1β. MIM-seq treatment restored collagen production levels in both models. These promising preclinical findings suggest that MIM-seq should be further investigated for periodontitis treatment.
ClNAC100 Is a NAC Transcription Factor of Chinese Fir in Response to Phosphate Starvation
Authors: Zhao Yuxuan , Huang Shuotian , Wei Lihui , Li Meng , Cai Tingting , Ma Xiangqing , Shuai Peng
Published: June 2023

Phosphate (Pi) deficiency is one of the most limiting factors for Chinese fir growth and production. Moreover, continuous cultivation of Chinese fir for multiple generations led to the reduction of soil nutrients, which hindered the yield of Chinese fir in southern China. Although NAC (NAM, ATAF, and CUC) transcription factors (TFs) play critical roles in plant development and abiotic stress resistance, it is still unclear how they regulate the response of Chinese fir to phosphate (Pi) starvation. Based on Pi-deficient transcriptome data of Chinses fir root, we identified a NAC transcription factor with increased expression under Pi deficiency, which was obtained by PCR and named ClNAC100. RT-qPCR confirmed that the expression of ClNAC100 in the root of Chinese fir was induced by phosphate deficiency and showed a dynamic change with time. It was positively regulated by ABA and negatively regulated by JA, and ClNAC100 was highly expressed in the roots and leaves of Chinese fir. Transcriptional activation assay confirmed that ClNAC100 was a transcriptional activator. The promoter of ClNAC100 was obtained by genome walking, which was predicted to contain a large number of stress, hormone, and growth-related cis-elements. Tobacco infection was used to verify the activity of the promoter, and the core promoter was located between −1519 bp and −589 bp. We identified 18 proteins bound to the ClNAC100 promoter and 5 ClNAC100 interacting proteins by yeast one-hybrid and yeast two-hybrid, respectively. We speculated that AHL and TIFY family transcription factors, calmodulin, and E3 ubiquitin ligase in these proteins might be important phosphorus-related proteins. These results provide a basis for the further study of the regulatory mechanism and pathways of ClNAC100 under Pi starvation.
Boosting the Photocatalysis of Plasmonic Au-Cu Nanocatalyst by AuCu-TiO2 Interface Derived from O2 Plasma Treatment
Authors: Zhu Bin , Li Xue , Li Yecheng , Liu Jinglin , Zhang Xiaomin
Published: June 2023

Plasmonic gold (Au) and Au-based nanocatalysts have received significant attention over the past few decades due to their unique visible light (VL) photocatalytic features for a wide variety of chemical reactions in the fields of environmental protection. However, improving their VL photocatalytic activity via a rational design is prevalently regarded as a grand challenge. Herein we boosted the VL photocatalysis of the TiO2-supported Au-Cu nanocatalyst by applying O2 plasma to treat this bimetallic plasmonic nanocatalyst. We found that O2 plasma treatment led to a strong interaction between the Au and Cu species compared with conventional calcination treatment. This interaction controlled the size of plasmonic metallic nanoparticles and also contributed to the construction of AuCu-TiO2 interfacial sites by forming AuCu alloy nanoparticles, which, thus, enabled the plasmonic Au-Cu nanocatalyst to reduce the Schottky barrier height and create numbers of highly active interfacial sites. The catalyst’s characterizations and density functional theory (DFT) calculations demonstrated that boosted VL photocatalytic activity over O2 plasma treated Au-Cu/TiO2 nanocatalyst arose from the favorable transfer of hot electrons and a low barrier for the reaction between CO and O with the construction of large numbers of AuCu-TiO2 interfacial sites. This work provides an efficient approach for the rational design and development of highly active plasmonic Au and Au-based nanocatalysts and deepens our understanding of their role in VL photocatalytic reactions.
The Adaptive Mechanisms and Checkpoint Responses to a Stressed DNA Replication Fork
Authors: Saldanha Joanne , Rageul Julie , Patel Jinal A. , Kim Hyungjin
Published: June 2023

DNA replication is a tightly controlled process that ensures the faithful duplication of the genome. However, DNA damage arising from both endogenous and exogenous assaults gives rise to DNA replication stress associated with replication fork slowing or stalling. Therefore, protecting the stressed fork while prompting its recovery to complete DNA replication is critical for safeguarding genomic integrity and cell survival. Specifically, the plasticity of the replication fork in engaging distinct DNA damage tolerance mechanisms, including fork reversal, repriming, and translesion DNA synthesis, enables cells to overcome a variety of replication obstacles. Furthermore, stretches of single-stranded DNA generated upon fork stalling trigger the activation of the ATR kinase, which coordinates the cellular responses to replication stress by stabilizing the replication fork, promoting DNA repair, and controlling cell cycle and replication origin firing. Deregulation of the ATR checkpoint and aberrant levels of chronic replication stress is a common characteristic of cancer and a point of vulnerability being exploited in cancer therapy. Here, we discuss the various adaptive responses of a replication fork to replication stress and the roles of ATR signaling that bring fork stabilization mechanisms together. We also review how this knowledge is being harnessed for the development of checkpoint inhibitors to trigger the replication catastrophe of cancer cells.
Tissue-Specific Hormone Signalling and Defence Gene Induction in an In Vitro Assembly of the Rapeseed Verticillium Pathosystem
Authors: Hafiz Fatema Binte , Geistlinger Joerg , Al Mamun Abdullah , Schellenberg Ingo , Neumann Günter , Rozhon Wilfried
Published: June 2023

Priming plants with beneficial microbes can establish rapid and robust resistance against numerous pathogens. Here, compelling evidence is provided that the treatment of rapeseed plants with Trichoderma harzianum OMG16 and Bacillus velezensis FZB42 induces defence activation against Verticillium longisporum infection. The relative expressions of the JA biosynthesis genes LOX2 and OPR3, the ET biosynthesis genes ACS2 and ACO4 and the SA biosynthesis and signalling genes ICS1 and PR1 were analysed separately in leaf, stem and root tissues using qRT-PCR. To successfully colonize rapeseed roots, the V. longisporum strain 43 pathogen suppressed the biosynthesis of JA, ET and SA hormones in non-primed plants. Priming led to fast and strong systemic responses of JA, ET and SA biosynthesis and signalling gene expression in each leaf, stem and root tissue. Moreover, the quantification of plant hormones via UHPLC-MS analysis revealed a 1.7- and 2.6-fold increase in endogenous JA and SA in shoots of primed plants, respectively. In roots, endogenous JA and SA levels increased up to 3.9- and 2.3-fold in Vl43-infected primed plants compared to non-primed plants, respectively. Taken together, these data indicate that microbial priming stimulates rapeseed defence responses against Verticillium infection and presumably transduces defence signals from the root to the upper parts of the plant via phytohormone signalling.
Early Cytomegalovirus Reactivation in Renal Recipients Is Associated with High Levels of B Cell Maturation Antigen Transcript Expression Prior to Transplantation
Authors: Alfaro Rafael , Rodríguez-Aguilar Luis , Llorente Santiago , Jimenez-Coll Victor , Martínez-Banaclocha Helios , Galián José Antonio , Botella Carmen , Moya-Quiles María Rosa , Muro-Perez Manuel , Minguela Alfredo , Legaz Isabel , Muro Manuel
Published: June 2023

Cytomegalovirus (CMV) infection is the most frequent infection episode in kidney transplant (KT) recipients. Reactivation usually occurs in the first three months after transplantation and is associated with higher cellular and/or antibody-mediated rejection rates and poorer graft performance. CMV induces the expression of BAFF (B-cell-activating factor, a cytokine involved in the homeostasis of B cells), which communicates signals for survival and growth to B cells and virus-specific plasma cells via the R-BAFF (BAFF receptor), TACI (the calcium modulator, the cyclophilin ligand interactor), and BCMA (B cell maturation antigen) receptors. These molecules of the BAFF system have also been suggested as biomarkers for the development of alloantibodies and graft dysfunction. This prospective study included 30 CMV-IgG seropositive KT recipients. The expression levels of the genes BAFF-R, transmembrane activator and CAML interactor (TACI), and B cell maturation antigen (BCMA) in peripheral blood leukocytes (PBL) pre-KT were determined using qPCR. qPCR was also used to monitor CMV reactivation in the first three months following KT. The remainder of the KT recipients were classified as CMV− reactivation, and those with more than 500 copies/mL in at least one sample were classified as CMV+ reactivation. There were no discernible variations in the BAFF-R and TACI transcript expression levels. In the CMV+ group, we examined the relationship between the transcript levels and peak viremia. Peak viremia levels and BCMA transcript levels showed a strong correlation. BAFF-R and TACI expressions showed no measurable differences. In patients with early CMV reactivation, high BCMA receptor expression was associated with increased plasmablast, lymphocyte B cell class-switched levels (LBCS), and viral load. Our findings demonstrate that pre-KT BCMA transcript levels increased in KT recipients with early CMV reactivation. These transcript levels positively correlate with peak viremia and weakly with plasmablast and LBCS levels in PBLs.
Mitochondria-Targeted Delivery Strategy of Dual-Loaded Liposomes for Alzheimer’s Disease Therapy
Authors: Vasileva Leysan , Gaynanova Gulnara , Valeeva Farida , Belyaev Grigory , Zueva Irina , Bushmeleva Kseniya , Sibgatullina Guzel , Samigullin Dmitry , Vyshtakalyuk Alexandra , Petrov Konstantin , Zakharova Lucia , Sinyashin Oleg
Published: June 2023

Liposomes modified with tetradecyltriphenylphosphonium bromide with dual loading of α-tocopherol and donepezil hydrochloride were successfully designed for intranasal administration. Physicochemical characteristics of cationic liposomes such as the hydrodynamic diameter, zeta potential, and polydispersity index were within the range from 105 to 115 nm, from +10 to +23 mV, and from 0.1 to 0.2, respectively. In vitro release curves of donepezil hydrochloride were analyzed using the Korsmeyer–Peppas, Higuchi, First-Order, and Zero-Order kinetic models. Nanocontainers modified with cationic surfactant statistically better penetrate into the mitochondria of rat motoneurons. Imaging of rat brain slices revealed the penetration of nanocarriers into the brain. Experiments on transgenic mice with an Alzheimer’s disease model (APP/PS1) demonstrated that the intranasal administration of liposomes within 21 days resulted in enhanced learning abilities and a reduction in the formation rate of Aβ plaques in the entorhinal cortex and hippocampus of the brain.
HRD Testing of Ovarian Cancer in Routine Practice: What Are We Dealing With?
Authors: Kekeeva Tatiana , Andreeva Yulia , Tanas Alexander , Kalinkin Alexey , Khokhlova Svetlana , Tikhomirova Tatiana , Tyulyandina Alexandra , Popov Anatoly , Kuzmenko Maria , Volkonsky Mikhail , Chernorubashkina Natalia , Saevets Valeria , Dmitriev Vadim , Nechushkina Valentina , Vedrova Olga , Andreev Sergei , Kutsev Sergey , Strelnikov Vladimir
Published: June 2023

Assessment of homologous recombination deficiency (HRD) status is now essential for ovarian cancer patient management. The aim of our study was to analyze the influence of ethnic variations, tumor purity, and neoadjuvant chemotherapy (CT) on the determination of HRD scores as well as to evaluate feasibility of HRD testing with the Amoy HRD Focus Assay in routine clinical practice. The HRD status, including the BRCA status and genomic scar score (GSS), was analyzed in 452 ovarian cancer specimens. The successful rate of HRD testing was 86% (388/452). The BRCA mutational rate was 29% (114/388); 252 samples (65%) were classified as HRD-positive. Our data demonstrate the feasibility of internal HRD testing by the AmoyDx HRD Focus Panel for high-grade serous ovarian cancer (HGSOC), showing results similar to other methods. The HRD rate in the Russian population is very similar to those of other European populations, as is the BRCA mutation frequency. The most substantial contribution to HRD level diversity is testing criteria depending on intrahospital arrangements. The analysis shows that biallelic BRCA alterations had higher GSS compared with those with monoallelic inactivation, consistent with positive HRD status. The study indicates that grades 1–2 of the pathological response caused by chemotherapy affect HRD scores and suggests controlling for tumor purity of 40% or more as a critical factor for GSS measurement.
Bioinformatic Identification of Potential RNA Alterations on the Atrial Fibrillation Remodeling from Human Pulmonary Veins
Authors: Igarashi Wataru , Takagi Daichi , Okada Daigo , Kobayashi Daiki , Oka Miho , Io Toshiro , Ishii Kuniaki , Ono Kyoichi , Yamamoto Hiroshi , Okamoto Yosuke
Published: June 2023

Atrial fibrillation (AF) is the most frequent persistent arrhythmia. Many genes have been reported as a genetic background for AF. However, most transcriptome analyses of AF are limited to the atrial samples and have not been evaluated by multiple cardiac regions. In this study, we analyzed the expression levels of protein-coding and long noncoding RNAs (lncRNAs) in six cardiac regions by RNA-seq. Samples were donated from six subjects with or without persistent AF for left atria, left atrial appendages, right atria, sinoatrial nodes, left ventricles, right ventricles, and pulmonary veins (PVs), and additional four right atrial appendages samples were collected from patients undergoing mitral valve replacement. In total, 23 AF samples were compared to 23 non-AF samples. Surprisingly, the most influenced heart region in gene expression by AF was the PV, not the atria. The ion channel-related gene set was significantly enriched upon analysis of these significant genes. In addition, some significant genes are cancer-related lncRNAs in PV in AF. A co-expression network analysis could detect the functional gene clusters. In particular, the cancer-related lncRNA, such as SAMMSON and FOXCUT, belong to the gene network with the cancer-related transcription factor FOXC1. Thus, they may also play an aggravating role in the pathogenesis of AF, similar to carcinogenesis. In the least, this study suggests that (1) RNA alteration is most intense in PVs and (2) post-transcriptional gene regulation by lncRNA may contribute to the progression of AF. Through the screening analysis across the six cardiac regions, the possibility that the PV region can play a role other than paroxysmal triggering in the pathogenesis of AF was demonstrated for the first time. Future research with an increase in the number of PV samples will lead to a novel understanding of the pathophysiology of AF.
An Update on MYBPC3 Gene Mutation in Hypertrophic Cardiomyopathy
Authors: Tudurachi Bogdan-Sorin , Zăvoi Alexandra , Leonte Andreea , Țăpoi Laura , Ureche Carina , Bîrgoan Silviu Gabriel , Chiuariu Traian , Anghel Larisa , Radu Rodica , Sascău Radu Andy , Stătescu Cristian
Published: June 2023

Hypertrophic cardiomyopathy (HCM) is the most prevalent genetically inherited cardiomyopathy that follows an autosomal dominant inheritance pattern. The majority of HCM cases can be attributed to mutation of the MYBPC3 gene, which encodes cMyBP-C, a crucial structural protein of the cardiac muscle. The manifestation of HCM’s morphological, histological, and clinical symptoms is subject to the complex interplay of various determinants, including genetic mutation and environmental factors. Approximately half of MYBPC3 mutations give rise to truncated protein products, while the remaining mutations cause insertion/deletion, frameshift, or missense mutations of single amino acids. In addition, the onset of HCM may be attributed to disturbances in the protein and transcript quality control systems, namely, the ubiquitin–proteasome system and nonsense-mediated RNA dysfunctions. The aforementioned genetic modifications, which appear to be associated with unfavorable lifelong outcomes and are largely influenced by the type of mutation, exhibit a unique array of clinical manifestations ranging from asymptomatic to arrhythmic syncope and even sudden cardiac death. Although the current understanding of the MYBPC3 mutation does not comprehensively explain the varied phenotypic manifestations witnessed in patients with HCM, patients with pathogenic MYBPC3 mutations can exhibit an array of clinical manifestations ranging from asymptomatic to advanced heart failure and sudden cardiac death, leading to a higher rate of adverse clinical outcomes. This review focuses on MYBPC3 mutation and its characteristics as a prognostic determinant for disease onset and related clinical consequences in HCM.
Screening Method and Antibacterial Activity of 1,3,4-Oxadiazole Sulfone Compounds against Citrus Huanglongbing
Authors: Luo Xin , Zhang Yong , Liu Xing , Zou Yue , Song Hongyi , Wang Sheng , Chen Jixiang
Published: June 2023

Citrus Huanglongbing (HLB) is one of the most destructive diseases in the citrus industry. At present, Candidatus Liberibacter asiaticus (CLas) cannot be cultured in vitro, and there is a lack of rapid methods to test antibacterial activity, which greatly hinders the discovery of new antibacterial agents against HLB. To establish a rapid screening method for antibacterial agents against HLB with simple operation, a short cycle, and a large number of tests, the CLas contents in leaves from different citrus branches, different leaves from the same citrus branch, and two halves of the same citrus leaf were detected. Compared with the leaves on different branches and different leaves on the same branch, the difference in CLas content of the left and right halves of the same leaf was small; the difference was basically between 0.7 and 1.3. A rapid and efficient method for primary screening agents against HLB termed the “half-leaf method” was established through our long-term optimization and improvement. To verify the stability and reliability of the activity data measured using this method, 6-chloropurine riboside, which is highly soluble in water, was used as the test agent, and its antibacterial activity against HLB was tested 45 times. The results of the antibacterial activity test showed little difference in the mean values of each data group, indicating that this method could be used as a rapid method for screening agents against HLB. We used this method to test the antibacterial activity of compounds synthesized by our research group against HLB and found that some of the compounds showed good activity.
Challenges and Future Perspectives for Additively Manufactured Polylactic Acid Using Fused Filament Fabrication in Dentistry
Authors: Kharmanda Ghais
Published: June 2023

Additive manufacturing (AM), which is also called rapid prototyping/3D printing/layered manufacturing, can be considered as a rapid conversion between digital and physical models. One of the most used materials in AM is polylactic acid (PLA), which has advantageous material properties such as biocompatibility, biodegradability, and nontoxicity. For many medical applications, it is considered as a leading biomaterial. In dentistry, in addition to its uses in dental models (education, teaching, simulation needs), it can be used for therapeutic objectives and tissue engineering. The fused filament fabrication (FFF) technique, also called fused deposition modeling (FDM), is widely used as an AM technique to perform complex and functional geometries directly from CAD files. In this review, the objective was to present the different challenges and future perspectives of this additively manufactured material by using FFF in dentistry areas. Some suggestions for future directions to extend to more dental applications (support structures, lattice structures, etc.) and to consider more criteria (sustainability, uncertainty etc.) will be discussed. Advanced studies such as machine learning (ML) techniques will be suggested to reduce the failure cases when using the additively manufactured PLA by FFF in dentistry.
Delayed Effect of Dry-Land Strength Training Sessions on Swimming Performance
Authors: Tsoltos Alexandros , Arsoniadis Gavriil , Tsolakis Charilaos , Koulouvaris Panagiotis , Simeonidis Theocharis , Chatzigiannakis Alexandros , Toubekis Argyris
Published: June 2023

The purpose of the study was to examine the effects of dry-land strength endurance (SE) and maximum strength (MS) sessions on next-day swimming performance. Eight swimmers (age: 18.6 ± 2.9 years) performed evening training sessions (19:00–19:40), including: (i) SE (2 × 15 − 20 repetitions, 50% of 1-RM), (ii) MS (2 × 5 repetitions, 90% of 1-RM), (iii) control (CON: no dry-land training). All sessions were followed by a 90-min swimming training (20:00–21:30). Medicine ball throw and countermovement jump, free countermovement jump and squat jump were evaluated before and after the dry-land training session and 12 h later, before a 100-m front crawl sprint (next day at 8:30 a.m.). Performance time, RPE, blood lactate and biomechanical variables in 100-m sprint were no different between conditions (time, MS: 64.70 ± 7.35, SE: 63.81 ± 7.29, CON: 64.52 ± 7.71 s, p > 0.05). Jump height was not changed before and after dry-land and before the 100-m sprint in all conditions (p > 0.05). Medicine ball throw was lower in MS compared to CON before the 100-m sprint (MS: 4.44 ± 1.11, vs. CON: 4.66 ± 1.21 m, p < 0.05). Upper-body but not lower- body muscle function may be affected by MS training. However, performance in a 100-m test is not affected by dry-land training performed 12 h earlier.
Mid- to Long-Term Survival of Geriatric Patients with Primary Septic Arthritis of the Shoulder: A Retrospective Study over a Period of 20 Years
Authors: Rüther Johannes , Taubert Lars , Loose Kim , Willauschus Maximilian , Silawal Sandeep , Millrose Michael , Bail Hermann Josef , Geßlein Markus
Published: June 2023

Septic arthritis of the shoulder is an urgent medical emergency that often occurs in elderly patients and is associated with high morbidity and mortality. Retrospectively, 56 patients aged ≥60 years, treated for primary septic monoarthritis of the shoulder at a maximum care hospital between 1 July 2001, and 30 July 2022, were included in this study. The primary aim of the study was analyzing survival rates and different bacteria in these patients. For statistical analysis, Kaplan–Meier curves were used for survival probability and the log-rank test was used to compare a survival probability of 5 years. The mean patient age was 78.7 years and a mean follow-up time of 3011.8 days. The mean survival of the entire study population was 920.3 days or 2.5 years. Significantly impaired 5-year survival was found only with increasing age and higher American Society of Anesthesiologists (ASA) physical status (PS) classification scores. Eight different types of bacteria were detected in the synovial fluid cultures. A total of 42 of 48 overall pathogens was Gram-positive and 6 were Gram-negative bacteria. Staphylococcus aureus was identified as the most frequent variant. We conclude that the mean survival is significantly shortened within the first 5 years with increasing age and ASA PS classification.
Effects of 3d Transition Metal Substitutions on the Phase Stability and Mechanical Properties of Ti–5.5Al–11.8[Mo]eq Alloys
Authors: Nohira Naoki , Widyanisa Keiko , Chiu Wan-Ting , Umise Akira , Tahara Masaki , Hosoda Hideki
Published: June 2023

The phase stability, mechanical properties, and functional properties of Ti–5.5Al–11.8[Mo]eq alloys are focused on in this study by substituting 3d transition metal elements (V, Cr, Co, and Ni) for Mo as β-stabilizers to achieve similar β phase stability and room temperature (RT) superelasticity. The ternary alloy systems with the equivalent chemical compositions of Ti–5.5Al–17.7V, Ti–5.5Al–9.5Cr, Ti–5.5Al–7.0Co, and Ti–5.5Al–9.5Ni (mass%) alloys were selected as the target materials based on the Mo equivalent formula, which has been applied for the Ti–5.5Al–11.8Mo alloy in the literature. The fundamental mechanical properties and functionalities of the selected alloys were examined. The β phase was stabilized at RT in all alloys except for the Ti–Al–V alloy. Among all alloys, the Ti–Al–Ni alloy exhibited superelasticity in the cyclic loading–unloading tensile tests at RT. As a result, similar to the Ti–5.5Al–11.8Mo mother alloy, by utilizing the Mo equivalent formula to substitute 3d transition metal elements for Mo, a RT superelasticity was successfully imposed.
Optimization of Micro-Drilling of Laminated Aluminum Composite Panel (Al–PE) Using Taguchi Orthogonal Array Design
Authors: Yalçın Bekir , Yüksel Ali , Aslantaş Kubilay , Der Oguzhan , Ercetin Ali
Published: June 2023

Aluminum Matrix Composite (AMC) represents an innovative class of materials that is extensively utilized in industries such as automotive, defense, aerospace, structural engineering, sports, and electronics. This study investigates the thrust force, exit burr formation, changes in the micro-tool, and drilled hole diameters during the micro-drilling of an aluminum-polyethylene composite panel (Al–PE). The panel consists of 3501 series aluminum skin materials bonded to a polyethylene (PE) core. Micro-drilling test parameters were designed using Taguchi’s L16 (42 23) orthogonal array. Tests were conducted with five control parameters: cutting speed with four levels (10 m/min, 20 m/min, 30 m/min, 40 m/min), feed rate with four levels (0.5 µm/rev, 1 µm/rev, 2 µm/rev, 4 µm/rev), the tool diameter with two levels (0.7 mm, 1 mm), and tool point angle with two levels (100°, 140°) using both AlTiN-coated and uncoated drills. The maximum thrust force (Fz), maximum burr height, and changes in both the drill tool and hole diameters were measured for analysis of variance (ANOVA). The results showed that, in terms of impact on Fz, tool point angle had the highest positive influence (64.54%) on the micro-drill at the entrance of composite (upper aluminum plate). The cutting speed had the highest positive influence (45.32%) on the tool in the core layer (PE core layer). The tool point angle also had the highest positive influence (68.95%) on the micro-drill at the lower layer of the composite (the lower aluminum plate). There was noticeable chip adhesion on the major cutting edge and nose area under micro-drilling conditions with higher thrust forces and burr height. The AlTiN coating had a positive effect on tool wear and hole diameter deviations, but it adversely affected the burr height.
Microstructure Evolution and Deformation Behavior of Extruded Mg-5Al-0.6Sc Alloy during Room and Elevated Temperature Tension Revealed by Ex-Situ EBSD and VPSC
Authors: Zhang Lei , Luan Shiyu , Yuan Shuai , Wang Jinhui , Chen Lijia , Jin Peipeng
Published: June 2023

In this study, the microstructure evolution and deformation behavior of the extruded Mg-5Al-0.6Sc (AS51) alloy during tensile testing at room temperature (RT) and 250 °C were investigated by electron backscattered diffraction (EBSD) characterization and Visco Plastic Self Consistent (VPSC) simulation. The results showed that a continuous hardening behavior of the alloy occurred during the deformation at RT, and a certain softening was caused by the occurrence of dynamic recovery (DRV) and dynamic recrystallization (DRX) in the late stage of deformation at 250 °C. The primary deformation mechanism at both RT and 250 °C was dislocation slip, with prismatic  slip being the dominant deformation mode, and no significant changes in grain size or texture type occurred. By identifying the activated twin variants, the results indicated that the selection of twin variants was closely related to the local stress concentration. The relatively low activation frequency of extension twinning at 250 °C is partly attributed to the fact that the consumption of dislocations by DRV and DRX can effectively relax the local stress concentration. Meanwhile, the DRX mechanism during the deformation of the alloy at 250 °C was mainly discontinuous dynamic recrystallization (DDRX), with a low recrystallization fraction.
Exploring the Blends’ Miscibility of a Novel Chitosan Derivative with Enhanced Antioxidant Properties; Prospects for 3D Printing Biomedical Applications
Authors: Michailidou Georgia , Zamboulis Alexandra , Bikiaris Dimitrios N.
Published: June 2023

Chitosan is a polysaccharide vastly examined in polymer science for its unique structure. In the present study, CS was derivatized with 2-methoxy-4vinylphenol (MVP) in four different ratios through a free radical reaction. The CS-MVP derivatives were characterized through FTIR, 1H-NMR, XRD, swelling, and solubility measurements. Owing to the enhanced antioxidant character of the MVP monomer, the antioxidant activity of the CS-MVP derivatives was assessed. In the optimum CS-MVP ratio, blends between CS and CS-MVP were prepared in ratios of 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, and 10:90 w/w, and their miscibility was examined by scanning electron microscopy (SEM) and viscosity measurements. In the optimum ratios, highly concentrated inks were prepared, and their viscosity measurements revealed the successful formation of highly viscous gels with shear thinning behavior. These inks could be appropriate candidates for biomedical and drug delivery applications.
Structural Anisotropy Parameters’ Effect on the Low-Temperature Impact Strength of Alloy Steels in Rolled Products
Authors: Kovalyova Tatyana , Issagulov Aristotel , Kovalev Pavel , Kulikov Vitaliy , Kvon Svetlana , Arinova Saniya
Published: June 2023

The present work is devoted to the study of the influence of the parameters of the structural anisotropy of rolled products on the low-temperature impact strength of alloyed steels. A quantitative metallographic analysis of the microstructure of rolled steel samples obtained after testing for low-temperature toughness was carried out. It was established that the main reason for the decrease in the low-temperature impact strength of rolled steel samples is a highly developed segregation band enriched with carbon films formed at the stage of steelmaking conversion in violation of the technology of continuous casting of steel. The microstructural analysis of rolled stock samples was used in the work, and studies of the fracture surface of rolled stock samples were carried out with a scanning electron microscope using X-ray microanalysis methods. The studies carried out showed that the metallurgical quality of sheets of one heat, as well as individual samples within one sheet, varied over a wide range, from satisfactory to unacceptably low. It was established that the main reason for the decreasing low-temperature impact strength of rolled products was a highly developed segregation band enriched with carbon films, formed at the stage of steelmaking in case of violation of the continuous casting of steel technology. The multivariate statistical analysis carried out showed that only the size of the segregation band has an effect on the low-temperature impact strength of 10 mm thick rolled coil samples.
A SiC Planar MOSFET with an Embedded MOS-Channel Diode to Improve Reverse Conduction and Switching
Authors: Li Ping , Guo Jingwei , Hu Shengdong , Lin Zhi
Published: June 2023

A novel split-gate SiC MOSFET with an embedded MOS-channel diode for enhanced third-quadrant and switching performances is proposed and studied using TCAD simulations in this paper. During the freewheeling period, the MOS-channel diode with a low potential barrier constrains the reverse current flow through it. Therefore, the suggested device not only has a low diode cut-in voltage but also entirely suppresses the intrinsic body diode, which will cause bipolar deterioration. In order to clarify the barrier-lowering effect of the MOS-channel diode, an analytical model is proposed. The calibrated simulation results demonstrate that the diode cut-in voltage of the proposed device is decreased from the conventional voltage of 2.7 V to 1.2 V. In addition, due to the split-gate structure, the gate-to-drain charge (QGD) of the proposed device is 20 nC/cm2, and the reverse-transfer capacitance (CGD) is 14 pF/cm2, which are lower than the QGD of 230 nC/cm2 and the CGD of 105 pF/cm2 for the conventional one. Therefore, a better high-frequency figure-of-merit and lower switching loss are obtained.
A Triple Band Substrate Integrated Waveguide with Dielectric Resonator Antenna for 4G and 5G Applications
Authors: Cheh Lin Irene Kong , Jamaluddin Mohd Haizal , Gaya Abinash
Published: June 2023

A triple-band substrate integrated waveguide (SIW) with dielectric resonator antenna (DRA) for fourth-generation (4G) and fifth-generation (5G) applications is proposed and analyzed in this paper. Loading SIW with DRA allows for a wide bandwidth, low losses, and fabrication ease. The proposed antenna can transmit and receive data independently by covering LTE Band 3 at 1.8 GHz, LTE Band 8 at 2.6 GHz, and 5G n77 at 3.7 GHz. A U-shaped cut is applied to achieve the targeted multi-resonance frequencies. The antenna obtains high bandwidths of up to 19.50% with 4.9 dBi gain and 81.0% efficiency at 1.8 GHz, 6.58% bandwidth with 4.4 dBi and 72.7% efficiency at 2.6 GHz, and 8.21% bandwidth with 6.7 dBi and 73.5% efficiency at 3.7 GHz. The simulated and measured results agree well. The proposed antenna is feasible for 4G and 5G applications.
Mapping Abandoned Uranium Mine Features Using Worldview-3 Imagery in Portions of Karnes, Atascosa and Live Oak Counties, Texas
Authors: Hubbard Bernard E. , Gallegos Tanya J. , Stengel Victoria
Published: June 2023

Worldview-3 (WV3) 16-band multispectral data were used to map exposed bedrock and mine waste piles associated with legacy open-pit mining of sandstone-hosted roll-front uranium deposits along the South Texas Coastal Plain. We used the “spectral hourglass” approach to extract spectral endmembers representative of these features from the image. This approach first requires calibrating the imagery to reflectance, then masking for vegetation, followed by spatial and spectral data reduction using a principal component analysis-based procedure that reduces noise and identifies homogeneous targets which are “pure” enough to be considered spectral endmembers. In this case, we used a single WV3 image which covered an ~11.5 km by ~19.5 km area of Karnes, Atascosa and Live Oak Counties, underlain by mined rocks from the Jackson Group and Catahoula Formation. Up to 58 spectral endmembers were identified using a further multi-dimensional class segregation method and were used as inputs for spectral angle mapper (SAM) classification. SAM classification resulted in the identification of at least 117 mine- and mine waste-related features, most of which were previously unknown. Class similarity was further evaluated, and the dominant minerals in each class were identified by comparison to spectral libraries and measured samples of actual Jackson Group uranium host rocks. Redundant classes were eliminated, and SAM was run a second time using a reduced set of 23 endmembers, which were found to map these same features as effectively as using the full 58 set of endmembers, but with significantly reduced noise and spectral outliers. Our classification results were validated by evaluating detailed scale mapping of three known mine sites (Esse-Spoonamore, Wright-McCrady and Garbysch-Thane) with published ground truth information about the vegetation cover, extent of erosion and exposure of waste pile materials and/or geologic information about host lithology and mineralization. Despite successful demonstration of the utility of WV3 data for inventorying mine features, additional landscape features such as bare agricultural fields and oil and gas drill pads were also identified. The elimination of such features will require combining the spectral classification maps presented in this study with high-quality topographic data. Also, the spectral endmembers identified during the course of this study could be useful for larger-scale mapping efforts using additional well-calibrated WV3 images beyond the coverage of our initial study area.
Links between Ikaite Morphology, Recrystallised Ikaite Petrography and Glendonite Pseudomorphs Determined from Polar and Deep-Sea Ikaite
Authors: Schultz Bo , Huggett Jennifer , Ullmann Clemens , Kassens Heidemarie , Kölling Martin
Published: June 2023

Petrography of recrystallised ikaite from Ocean Drilling Program material has been presented previously from Nankai Trough and Congo (ex-Zaire) deep-sea fan. This paper expands on the Nankai Trough ikaite observations, drawing on evidence from Laptev Sea, South Georgia, Okhotsk Sea, and coastal lagoon Point Barrow. However, even though many ikaite and glendonite sites occur at high latitudes, it cannot be that ikaite forms exclusively in polar environments, as demonstrated by the occurrences in the low latitude low temperature deep sea sediments offshore Gulf of Guinea (Angola Congo) and mid-latitude deep-sea trenches offshore Japan. Recrystallised ikaite occurs as mm large, zoned calcite crystals in all samples, along with secondary phases of calcite. Our data set is unique in that the origin, storage, and recrystallisation process of natural formed ikaite is recorded in detail and confirms that glendonite petrographic characteristics are a consequence of the structure and chemistry of recrystallising ikaite and not the physical or geochemical environment. The transformation of man-made ikaite to calcite as recorded in laboratory studies, is a process very similar to the one we have observed for natural ikaite. Most significant is that there is variation in the order of the calcite types within a single sample, leading to the conclusion that the variation is a consequence of impurities and geochemical variability in the ikaite, not the external environment. Morphological observations reveal similarities in ikaite and glendonite, this and the similarity in internal textures in glendonite and recrystallised ikaite confirms that glendonite may be used as an indicator of past presence of ikaite.
Metal Mobility in Embryonic-to-Proto-Ni-Laterite Profiles from Non-Tropical Climates
Authors: González-Jiménez José María , Villanova-de-Benavent Cristina , Yesares Lola , Marchesi Claudio , Cartwright David , Proenza Joaquín A. , Monasterio-Guillot Luis , Gervilla Fernando
Published: June 2023

We evaluated the mobility of a wide suite of economic metals (Ni, Co, REE, Sc, PGE) in Ni-laterites with different maturities, developed in the unconventional humid/hyper-humid Mediterranean climate. An embryonic Ni-laterite was identified at Los Reales in southern Spain, where a saprolite profile of ~1.5 m thick was formed at the expense of peridotites of the subcontinental lithospheric mantle. In contrast, a more mature laterite was reported from Camán in south-central Chile, where the thicker (~7 m) weathering profile contains well-developed lower and upper oxide horizons. This comparative study reveals that both embryonic and mature laterites can form outside the typical (sub)-tropical climate conditions expected for lateritic soils, while demonstrating a similar chemical evolution in terms of major (MgO, Fe2O3, and Al2O3), minor (Ni, Mn, Co, Ti, Cr), and trace (REE, Y, Sc, PGE, Au) element concentrations. We show that, even in the earliest stages of laterization, the metal remobilization from primary minerals can already result in uneconomic concentration values.
Recovery of Rare Earth Elements from the Leaching Solutions of Spent NdFeB Permanent Magnets by Selective Precipitation of Rare Earth Oxalates
Authors: Klemettinen Anna , Adamski Zbigniew , Chojnacka Ida , Leśniewicz Anna , Rycerz Leszek
Published: June 2023

After mechanical pre-treatment, the typical hydrometallurgical route of NdFeB magnet recycling starts with leaching in acidic solutions. However, due to the high concentration of iron ions in the leaching solution, the selective recovery of rare earths from the solution is challenging. In our work, the selective precipitation of rare earth oxalates as a potential separation method was proposed. The precipitation of neodymium oxalate was first tested on model solutions, which was then followed by experimental tests carried out on real solutions after the leaching of NdFeB magnets. The recovery of rare earths in the form of oxalates was investigated with the use of different amounts of oxalic acid in relation to its stoichiometric amount. The most efficient separation of rare earths was observed in the case where sulfuric acid was used for leaching. The use of oxalic acid in stoichiometric amounts resulted in the precipitation of about 93% of all rare earths present in the solution, whereas the concentration of Fe and other elements (Ni, Co, and B) practically did not change. An increase in oxalic acid of 20% and 40% more than the stoichiometric amount (100%) led to the increase in the precipitation efficiency of rare earths to 96.7% and 98.1%, respectively. However, the use of oxalic acid in a 1.4 ratio caused a 7% decrease in Fe concentration, which suggests Fe co-precipitation. In order to investigate a possibility of further increasing the separation of rare earths from iron, an additional method was tested, in which iron was first oxidized from Fe2+ to Fe3+ before the precipitation of rare earth oxalates.
The Role of Particle Size and Shape on the Recovery of Copper from Different Electrical and Electronic Equipment Waste
Authors: Burat Fırat , Dinç Nazlım İlkyaz , Dursun Halide Nur , Ulusoy Ugur
Published: June 2023

The increasing world population and the development of technology have boosted the demand for electrical and electronic equipment (EEE). Equipment that has completed its life cycle causes serious damage to the environment due to its toxic components. In addition, it contains many more base metals (copper, aluminum, nickel, lead, tin, etc.) and precious metals (silver, gold, palladium, platinum, etc.) compared with a run of mine ore. Recycling these values with an economic and environmental understanding will ensure sustainability and prevent the rapid depletion of natural resources. Specific gravity, magnetic, electrostatic, optical, surface, thermal, and other property differences between particles as well as the shape, size, and distribution of individual particles directly determine the success of the recycling process. By determining the behavior of the particles during enrichment and producing grains suitable for enrichment with better performance in the size reduction stage, the quality of the concentrate to be subjected to the final chemical/metallurgical treatment will be enhanced. The main aim of this study is to reveal the effect of particle size and shape properties on the recovery of valuable metals from two different waste electrical and electronic equipment (WEEE) sources, end-of-life printed circuit boards and waste electric wires, using environmentally friendly, easier-to-use, and cost-effective mechanical, physical, and physiochemical processes. Deciding on the most suitable enrichment process after detailed characterization of the products obtained from different comminution equipment and their particle size and shape directly affected the amount, content, and recovery of the final concentrate.
Effect of Steam on Carbonation of CaO in Ca-Looping
Authors: Bai Ruzhan , Li Na , Liu Quansheng , Chen Shenna , Liu Qi , Zhou Xing
Published: June 2023

Ca-looping is an effective way to capture CO2 from coal-fired power plants. However, there are still issues that require further study. One of these issues is the effect of steam on the Ca-looping process. In this paper, a self-madethermogravimetric analyzer that can achieve rapid heating and cooling is used to measure the change of sample weight under constant temperature conditions. The parameters of the Ca-looping are studied in detail, including the addition of water vapor alone in the calcination or carbonation stage and the calcination/carbonation reaction temperatures for both calcination and carbonation stages with water vapor. Steam has a positive overall effect on CO2 capture in the Ca-looping process. When steam is present in both calcination and carbonation processes, it increases the decomposition rate of CaCO3 and enhances the subsequent carbonation conversion of CaO. However, when steam was present only in the calcination process, there was lower CaO carbonation conversion in the following carbonation process. In contrast, when steam was present in the carbonation stage, CO2 capture was improved. Sample characterizations after the reaction showed that although water vapor had a negative effect on the pore structure, adding water vapor increased the diffusion coefficient of CO2 and the carbonation conversion rate of CaO.