bluuebunny/crossref_metadata_2025 huggingface dataset:
Safety and immunogenicity of a SARS-CoV-2 Gamma variant RBD-based protein adjuvanted vaccine used as booster in healthy adults
Authors: Pasquevich Karina A. , Coria Lorena M. , Ceballos Ana , Mazzitelli Bianca , Rodriguez Juan Manuel , Demaría Agostina , Pueblas Castro Celeste , Bruno Laura , Saposnik Lucas , Salvatori Melina , Varese Augusto , González Soledad , González Martínez Veronica V. , Geffner Jorge , Álvarez Diego ,   Laboratorio Pablo Cassará R&D and CMC for ARVAC CG consortium, del Priore Sabrina A. , Hernando Insua Andrés C. , Kaufmann Ingrid G. , Di María Adrián , Góngora Adrián , Moreno Agustín , Cervellini Susana , Martin Blasco , Ali Esteban , Albarracín Romina , Bruno Barsanti , Toneguzzo Fernando , Sasso Guillermina , Stamer Sebastian , Cardoso Regina , Chajet Alejandro , Feleder Ethel , Halabe Karina , Perez Lera Pablo E. , de Oca Federico Montes , Vega Julio C. , Lombardo Mónica , Yerino Gustavo A. , Fló Juan , Cassataro Juliana
Published: July 2023

A Gamma Variant RBD-based aluminum hydroxide adjuvanted vaccine called ARVAC CG was selected for a first in human clinical trial. Healthy male and female participants (18-55 years old) with a complete COVID-19-primary vaccine scheme were assigned to receive two intramuscular doses of either a low-dose or a high-dose of ARVAC CG. The primary endpoint was safety. The secondary objective was humoral immunogenicity. Cellular immune responses were studied as an exploratory objective. The trial was prospectively registered in PRIISA.BA (Registration Code 6564) and ANMAT and retrospectively registered in ClinicalTrials.gov (NCT05656508). Samples from participants of a surveillance strategy implemented by the Ministry of Health of the Province of Buenos Aires that were boosted with BNT162b2 were also analyzed to compare with the booster effect of ARVAC CG. ARVAC CG exhibits a satisfactory safety profile, a robust and broad booster response of neutralizing antibodies against the Ancestral strain of SARS-CoV-2 and the Gamma, Delta, Omicron BA.1 and Omicron BA.5 variants of concern and a booster effect on T cell immunity in individuals previously immunized with different COVID-19 vaccine platforms.
Thermo-electro-mechanical microstructural interdependences in conductive thermoplastics
Authors: Crespo-Miguel Javier , Lucarini Sergio , Arias Angel , Garcia-Gonzalez Daniel
Published: July 2023

Additive manufacturing has enabled the design of thermoplastic components that provide structural support, electrical conductivity and heat generation modulated by mechanical deformation. The mechanisms and interplays that govern the material response at the microstructural level remain, however, elusive. Here, we develop an experimental method to characterise conductive filaments from a combined mechanical, electrical and thermal perspective. This approach is used to unravel exciting material interplays of conductive polylactic acid. To overcome experimental limitations that prevent a complete microstructural analysis of the problem, we develop a full-field homogenisation framework and implement it for finite elements. The framework accounts for viscoplasticity, electrical and thermal conduction, convection and heat generation via Joule effect, as well as for the interdependences between them. After experimental validation, the framework is applied to virtually optimise fabrication requirements to obtain desired properties in final products, i.e., stiffer products, filaments with higher conductivities or with better sensing capabilities.
Annotated dataset for deep-learning-based bacterial colony detection
Authors: Makrai László , Fodróczy Bettina , Nagy Sára Ágnes , Czeiszing Péter , Csabai István , Szita Géza , Solymosi Norbert
Published: July 2023

Quantifying bacteria per unit mass or volume is a common task in various fields of microbiology (e.g., infectiology and food hygiene). Most bacteria can be grown on culture media. The unicellular bacteria reproduce by dividing into two cells, which increases the number of bacteria in the population. Methodologically, this can be followed by culture procedures, which mostly involve determining the number of bacterial colonies on the solid culture media that are visible to the naked eye. However, it is a time-consuming and laborious professional activity. Addressing the automation of colony counting by convolutional neural networks in our work, we have cultured 24 bacteria species of veterinary importance with different concentrations on solid media. A total of 56,865 colonies were annotated manually by bounding boxes on the 369 digital images of bacterial cultures. The published dataset will help developments that use artificial intelligence to automate the counting of bacterial colonies.
Suppression of VEGF and inflammatory cytokines, modulation of Annexin A1 and organ functions by galloylquinic acids in breast cancer model
Authors: Abd El-Salam Mohamed , El-Tanbouly Ghada , Bastos Jairo , Metwaly Heba
Published: July 2023

The ongoing development of novel drugs for breast cancer aims to improve therapeutic outcomes, reduce toxicities, and mitigate resistance to chemotherapeutic agents. Doxorubicin (Dox) is known for its significant side effects caused by non-specific cytotoxicity. In this study, we investigated the antitumor activity of galloylquinic acids (BF) and the beneficial role of their combination with Dox in an Ehrlich ascites carcinoma (EAC)-bearing mouse model, as well as their cytotoxic effect on MCF-7 cells. The EAC-mice were randomized into five experimental groups: normal saline, Dox (2 mg/kg, i.p), BF (150 mg/kg, orally), Dox and BF combined mixture, and a control group. Mice were subjected to a 14-day treatment regimen. Results showed that BF compounds exerted chemopreventive effects in EAC mice group by increasing mean survival time, decreasing tumor volume, inhibiting ascites tumor cell count, modulating body weight changes, and preventing multi-organ histopathological alterations. BF suppressed the increased levels of inflammatory mediators (IL-6 and TNF-α) and the angiogenic marker VEGF in the ascitic fluid. In addition, BF and their combination with Dox exhibited significant cytotoxic activity on MCF-7 cells by inhibiting cell viability and modulating Annexin A1 level. Moreover, BF treatments could revert oxidative stress, restore liver and kidney functions, and normalize blood cell counts.
The association between access to key household resources and violence against women
Authors: Bandyopadhyay Siddhartha , Sarkar Sanjukta , Sensarma Rudra
Published: July 2023

We provide the first comprehensive analysis of the association between two key household resources (drinking water and toilet access) and both non-partner violence (NPV) and intimate partner violence (IPV) experienced by women. We use data from a nationally representative household survey for India obtained from the latest (fourth) round of the National Family Health Survey conducted in 2015–16. We employ logistic regression method and also use inverse-probability-weighted regression adjustment to control for selection bias. We find that NPV decreases with access to drinking water, while IPV decreases with provision of toilets. These results are found to be robust to an alternative method viz. propensity score matching and selection on unobservables using the Rosenbaum bounds approach.
Distribution characteristics of organic carbon (nitrogen) content, cation exchange capacity, and specific surface area in different soil particle sizes
Authors: Bi Xiaoqian , Chu Hang , Fu Mingming , Xu Dandan , Zhao Wenyu , Zhong Yijian , Wang Mei , Li Ke , Zhang Ya-nan
Published: July 2023

Understanding the distribution of soil organic carbon and nitrogen (OC(N)) content, cation exchange capacity (CEC), and specific surface area (SSA) in different soil particle sizes is crucial for studying soil fertility and properties. In this study, we investigated the distribution characteristics of the OC(N), CECand SSA in different particles of yellow–brown soil under different methods. The result revealed that as the particle size decreased, the soil OC(N), SSA and CEC content gradually increase. The content of OC and ON different soil particles ranged from 1.50–28.16 g·kg−1 to 0.18–3.78 g·kg−1, respectively, and exhibited significant differences between different particles. We observed good linear relationships between OC and ON in different particle sizes of yellow–brown soil under different utilization methods, with correlation coefficients ranging from 0.86 to 0.98, reaching a very significant level (n = 12, p < 0.01). The ranges of SSA and CEC in different particles of the four soils were 0.30–94.70 m2·g−1 and 0.70–62.91 cmol·kg−1, respectively. Additionally, we found logarithmic relationships between SSA (CEC) and the equivalent diameter for the four soils, with correlation coefficients (r2) higher than 0.91. Furthermore, there was an extremely significant linear relationship between CEC and SSA of the four soils, with correlation coefficients (r2) of 0.92–0.97 (n = 12, p < 0.01). These results highlight the close relationship between soil particle size and soil OC(N), SSA, and CEC. The conclusions drawn from this study provide valuable data support and a theoretical basis for further understanding soil properties.
Differential exon usage of developmental genes is associated with deregulated epigenetic marks
Authors: Do Hoang Thu Trang , Shanak Siba , Barghash Ahmad , Helms Volkhard
Published: July 2023

Alternative exon usage is known to affect a large portion of genes in mammalian genomes. Importantly, different splice isoforms sometimes possess distinctly different protein functions. Here, we analyzed data from the Human Epigenome Atlas for 11 different human adult tissues and for 8 cultured cells that mimic early developmental stages. We found a significant enrichment of cases where differential usage of exons in various developmental stages of human cells and tissues is associated with differential epigenetic modifications in the flanking regions of individual exons. Many of the genes that were differentially regulated at the exon level and showed deregulated histone marks at the respective exon flanks are functionally associated with development and metabolism.
Full thickness 3D in vitro conjunctiva model enables goblet cell differentiation
Authors: Schwebler Julian , Fey Christina , Kampik Daniel , Lotz Christian
Published: July 2023

In vitro culture and generation of highly specialized goblet cells is still a major challenge in conjunctival 3D in vitro equivalents. A model comprising all physiological factors, including mucus-secreting goblet cells has the potential to act as a new platform for studies on conjunctival diseases. We isolated primary conjunctival epithelial cells and fibroblasts from human biopsies. 3D models were generated from either epithelial layers or a combination of those with a connective tissue equivalent. Epithelial models were investigated for marker expression and barrier function. Full-thickness models were analyzed for goblet cell morphology and marker expression via immunofluorescence and quantitative real-time PCR. Simple epithelial models cultured at the air–liquid interface showed stratified multi-layer epithelia with pathologic keratinization and without goblet cell formation. The combination with a connective tissue equivalent to generate a full-thickness model led to the formation of a non-keratinized stratified multi-layer epithelium and induced goblet cell differentiation. In our model, a high resemblance to natural conjunctiva was achieved by the combination of conjunctival epithelial cells with fibroblasts embedded in a collagen-hydrogel as connective tissue equivalent. In the future, our conjunctival in vitro equivalent enables the investigation of goblet cell differentiation, conjunctival pathologies as well as drug testing.
Comparison between the geological features of Venus and Earth based on gravity aspects
Authors: Karimi Kurosh , Kletetschka Gunther , Meier Verena
Published: July 2023

We probe the gravitational properties of two neighboring planets, Earth and Venus. To justify a comparison between gravity models of the two planets, spherical harmonic series were considered up to a degree and order of 100. The topography and gravity aspects, including $${\Gamma }_{zz}$$

Γ

zz

(vertical derivative of the vertical component of the gravity field), strike alignment (SA), comb factor (CF), and I2 invariant derived from the Marussi tensor, were calculated for the two planets at specifically selected zones that provided sufficient resolution. From Γzz we discovered that the N-NW edge of Lakshmi Planum does not show any subduction-like features. Its Γzz signature resembles passive continental margins on Earth, like those surrounding the Indian Peninsula. Moreover, according to SA and CF, the Pacific and Philippine-North American Contact Zone on Earth indicates significantly higher level of deformation due to convergent motion of the plates, whereas the deformation level on Venus is significantly smaller and local, when considering an equatorial rifting zone (ERZ) of Venus (between Atla-Beta Regios) as diverging boundaries. The strain mode on the East African Rift system is smaller in comparison with ERZ as its Venusian analog. The topography-I2 analysis suggests a complicated nature of the topographic rise on Beta Regio. We show that specific regions in this volcanic rise are in incipient stages of upward motion, with denser mantle material approaching the surface and thinning the crust, whereas some risen districts show molten and less dense underlying crustal materials. Other elevated districts appear to be due to mantle plumes and local volcanic activities with large density of underlying material.
Computational modeling and synthesis of pyridine variants of benzoyl-phenoxy-acetamide with high glioblastoma cytotoxicity and brain tumor penetration
Authors: Ingraham Charles H. , Stalinska Joanna , Carson Sean C. , Colley Susan B. , Rak Monika , Lassak Adam , Peruzzi Francesca , Reiss Krzysztof , Jursic Branko S.
Published: July 2023

Glioblastomas are highly aggressive brain tumors for which therapeutic options are very limited. In a quest for new anti-glioblastoma drugs, we focused on specific structural modifications to the benzoyl-phenoxy-acetamide (BPA) structure present in a common lipid-lowering drug, fenofibrate, and in our first prototype glioblastoma drug, PP1. Here, we propose extensive computational analyses to improve the selection of the most effective glioblastoma drug candidates. Initially, over 100 structural BPA variations were analyzed and their physicochemical properties, such as water solubility (− logS), calculated partition coefficient (ClogP), probability for BBB crossing (BBB_SCORE), probability for CNS penetration (CNS-MPO) and calculated cardiotoxicity (hERG), were evaluated. This integrated approach allowed us to select pyridine variants of BPA that show improved BBB penetration, water solubility, and low cardiotoxicity. Herein the top 24 compounds were synthesized and analyzed in cell culture. Six of them demonstrated glioblastoma toxicity with IC50 ranging from 0.59 to 3.24 µM. Importantly, one of the compounds, HR68, accumulated in the brain tumor tissue at 3.7 ± 0.5 µM, which exceeds its glioblastoma IC50 (1.17 µM) by over threefold.
Automatic electrode scalar location assessment after cochlear implantation using a novel imaging software
Authors: Geiger S. , Iso-Mustajärvi M. , Nauwelaers T. , Avci E. , Julkunen P. , Linder P. , Silvast T. , Dietz A.
Published: July 2023

As of today, image-based assessment of cochlear implant electrode array location is not part of the clinical routine. Low resolution and contrast of computer tomography (CT) imaging, as well as electrode array artefacts, prevent visibility of intracochlear structures and result in low accuracy in determining location of the electrode array. Further, trauma assessment based on clinical-CT images requires a uniform image-based trauma scaling. Goal of this study was to evaluate the accuracy of a novel imaging software to detect electrode scalar location. Six cadaveric temporal bones were implanted with Advanced Bionics SlimJ and Mid-Scala electrode arrays. Clinical-CT scans were taken pre- and postoperatively. In addition, micro-CTs were taken post-operatively for validation. The electrode scalar location rating done by the software was compared to the rating of two experienced otosurgeons and the micro-CT images. A 3-step electrode scalar location grading scale (0 = electrode in scala tympani, 1 = interaction of electrode with basilar membrane/osseous spiral lamina, 2 = translocation of electrode into scala vestibuli) was introduced for the assessment. The software showed a high sensitivity of 100% and a specificity of 98.7% for rating the electrode location. The correlation between rating methods was strong (kappa > 0.890). The software gives a fast and reliable method of evaluating electrode scalar location for cone beam CT scans. The introduced electrode location grading scale was adapted for assessing clinical CT images.
Quality evaluation of water disclosure of Chinese papermaking enterprises based on accelerated genetic algorithm
Authors: He Chaoran , Shen Juqin , Xu Jiawei , Sun Fuhua , Wang Bing
Published: July 2023

As the carrier of enterprise water resources management disclosure, water information disclosure is a means of expression of enterprises’ environmental responsibility. First, a corporate water information disclosure quality evaluation index system and evaluation method are established, and with the help of the projection tracing method of accelerated genetic algorithm, 27 paper companies in China are selected as a sample and the disclosure quality level is analyzed empirically. Then, the analysis is carried out in terms of three changes in vertical trends, horizontal trends and changes in laws, regulations and policies, and the results show that Chinese paper and paper product enterprises have low quality of water information disclosure, weak disclosure content and low voluntary disclosure. Finally, feasible suggestions are made based on the evaluation of disclosure issues.
Self-compassion is associated with the superior longitudinal fasciculus in the mirroring network in healthy individuals
Authors: Hwang Yeong-Geon , Pae Chongwon , Song Chae Rim , Kim Hyun-Ju , Bang Minji , Park Chun Il , Choi Tai Kiu , Kim Min-Kyoung , Lee Sang-Hyuk
Published: July 2023

Self-compassion (SC) involves taking an emotionally positive attitude towards oneself when suffering. Although SC has positive effects on mental well-being as well as a protective role in preventing symptoms in healthy individuals, few studies on white matter (WM) microstructures in neuroimaging studies of SC has been studied. Brain imaging data were acquired from 71 healthy participants. WM regions of mirroring network were analyzed using tract-based spatial statistics. After the WM regions associated with SC were extracted, exploratory correlation analysis with the self-forgiveness scale, the coping scale, and the world health organization quality of life scale abbreviated version was performed. We found that self-compassion scale total scores were negatively correlated with the fractional anisotropy (FA) values of the superior longitudinal fasciculus (SLF) in healthy individuals. The self-kindness and mindfulness subscale scores were also negatively correlated with FA values of the same regions. These FA values were negatively correlated with the total scores of self-forgiveness scale, and self-control coping strategy and confrontation coping strategy. Our findings suggest levels of SC may be associated with WM microstructural changes of SLF in healthy individuals. These lower WM microstructures may be associated with positive personal attitudes, such as self-forgiveness, self-control and active confrontational strategies.
Evaluation of hackberry (Celtis australis L.) fruits as sources of bioactive compounds
Authors: Safari Farkhondeh , Hassanpour Hamid , Alijanpour Ahmad
Published: July 2023

Hackberry (Celtisaustralis L.) is native to the Mediterranean region and is distributed in Europe, Turkey, North Africa, and Iran. To the best of our knowledge, no study has been conducted on C.australis L. in the Arasbaran region, Iran. In the present study, total phenol (TP), flavonoid (TF), antioxidant capacity based on DPPH and FRAP assays and phenolic compounds and sugars profiles were investigated. According to the results, the range of antioxidant capacity based on DPPH and FRAP assays was 14.12–88.24% and 44.35–117.87 mg Fe2+/100 g, respectively. Also, the range of gallic acid, caffeic acid, chlorogenic acid, rutin, p-coumaric acid, rosmaric acid, cinnamic acid, and apigenin content was 2.59–26.32, 2.03–9.32, 0.94–11.35, 1.80–4.857, 2.32–9.52, 4.74–51.38, 0.18–2.10 and 0.27–1.37 mg/g, respectively. The results of factor analysis showed that the C12, C14, C15, C20, C8, C16, C3, and C20 genotypes are positively characterized by the first principal component (PCA1) that have a higher caffeic acid, chlorogenic acid, rutin, p-coumaric acid, rosmaric acid, quercetin, cinnamic acid, and apigenin phenolic compounds. Based on cluster analysis, the twenty genotypes were located in 2 main clusters. In general, the obtained results can be useful for breeding programs and the introduction of cultivars in Celtisaustralis L.
Electrophysiological evidence for sensitization effects elicited by concurrent social threats
Authors: Niedeggen Michael , Fang Xu , Yang Yu-Fang , Kerschreiter Rudolf
Published: July 2023

Experiencing a social threat, such as social exclusion, is a painful event. In contrast to previous studies providing insight into the processing of a single short-termed threat, we exposed healthy individuals to the simultaneous onset of different social threats. This approach allowed us to track whether these threats are processed independently—or whether they interact in a common system. Using a virtual ball-throwing game (Cyberball), electrophysiological (event-related brain potentials, ERPs) and behavioral (self-reports) responses were collected. We assigned undergraduates to three experimental groups: single threat exclusion (n = 24), single threat loss of control (n = 26), and joint onset of both threats (dual-threat, n = 25). Self-reports indicated an increase in threats (i.e., in perceived exclusion and loss-of-control) in the latter group. The ERPs disentangled the neural responses to each threat: In the dual-threat group, the amplitudes of the P3 responses to exclusionary and intervention events were enhanced. This indicates that individuals are sensitized to each of the threats when the other threat is present simultaneously. Our findings support the theoretical notion of a common cognitive system responding to violations in subjective expectations.
Exploring the association between selective serotonin reuptake inhibitors and rhabdomyolysis risk based on the FDA pharmacovigilance database
Authors: Wang Yan , Lin Yajing , Lin Qing , Liang Haiming , Cai Weiming , Jiang Dongbo
Published: July 2023

Rhabdomyolysis is a syndrome potentially fatal and has been associated with selective serotonin reuptake inhibitors (SSRIs) treatment in a few case reports. Herein, we purpose to establish the correlation between SSRIs use and rhabdomyolysis using the U.S. Food and Drug Administration Adverse Event Reporting System (FAERS) database. We conducted an analysis on reports that were submitted to the FAERS database during the period between January 1, 2004, and December 31, 2022. Four algorithms, including reporting odds ratio (ROR), proportional reporting ratio (PRR), Bayesian confidence propagation neural network (BCPNN), and empirical Bayes geometric mean (EBGM), were employed to quantify the signals of rhabdomyolysis associated with SSRIs. In total, 16,011,277 non-duplicated reports were obtained and analyzed. Among 33,574 reports related to rhabdomyolysis, SSRIs were classified as primary suspected drug in 889 cases. Disproportionality analysis identified a positive signal between rhabdomyolysis and SSRIs (ROR: 2.86, 95% CI 2.67–3.05; PRR: 2.84, χ2: 1037.16; IC0.25 = 1.39; EBGM0.5 = 2.64). Among six SSRIs, fluvoxamine had the strongest signal (ROR: 11.64, 95% CI 8.00–16.93; PRR: 11.38, χ2: 265.51; IC0.25 = 2.41; EBGM0.5 = 8.31), whereas no significant signal of rhabdomyolysis was detected for paroxetine (ROR: 1.83, 95% CI 1.55–2.15; PRR: 1.82, χ2: 53.82; IC0.25 = 0.73; EBGM0.5 = 1.59). After excluding cases co-administered with statins, the signal of rhabdomyolysis associated with SSRIs remains significant. Our analysis reveals that there are differences in safety signals among six SSRIs in respect to the risk of rhabdomyolysis, with fluvoxamine displaying the highest risk signal, while paroxetine did not show a significant signal. Given the potentially lethal nature of rhabdomyolysis, healthcare professionals should inform patients of the potential risk of rhabdomyolysis associated with SSRIs prior to initiating treatment.
5-Aminosalicylic acid alters the gut microbiota and altered microbiota transmitted vertically to offspring have protective effects against colitis
Authors: Wada Haruka , Miyoshi Jun , Kuronuma Satoshi , Nishinarita Yuu , Oguri Noriaki , Hibi Noritaka , Takeuchi Osamu , Akimoto Yoshihiro , Lee Sonny T. M. , Matsuura Minoru , Kobayashi Taku , Hibi Toshifumi , Hisamatsu Tadakazu
Published: July 2023

Although many therapeutic options are available for inflammatory bowel disease (IBD), 5-aminosalicylic acid (5-ASA) is still the key medication, particularly for ulcerative colitis (UC). However, the mechanism of action of 5-ASA remains unclear. The intestinal microbiota plays an important role in the pathophysiology of IBD, and we hypothesized that 5-ASA alters the intestinal microbiota, which promotes the anti-inflammatory effect of 5-ASA. Because intestinal inflammation affects the gut microbiota and 5-ASA can change the severity of inflammation, assessing the impact of inflammation and 5-ASA on the gut microbiota is not feasible in a clinical study of patients with UC. Therefore, we undertook a translational study to demonstrate a causal link between 5-ASA administration and alterations of the intestinal microbiota. Furthermore, by rigorously controlling environmental confounders and excluding the effect of 5-ASA itself with a vertical transmission model, we observed that the gut microbiota altered by 5-ASA affected host mucosal immunity and decreased susceptibility to dextran sulfate sodium-induce colitis. Although the potential intergenerational transmission of epigenetic changes needs to be considered in this study, these findings suggested that alterations in the intestinal microbiota induced by 5-ASA directed the host immune system towards an anti-inflammatory state, which underlies the mechanism of 5-ASA efficacy.
Comparing the signaling and transcriptome profiling landscapes of human iPSC-derived and primary rat neonatal cardiomyocytes
Authors: Bourque Kyla , Jones-Tabah Jace , Pétrin Darlaine , Martin Ryan D. , Tanny Jason C. , Hébert Terence E.
Published: July 2023

The inaccessibility of human cardiomyocytes significantly hindered years of cardiovascular research efforts. To overcome these limitations, non-human cell sources were used as proxies to study heart function and associated diseases. Rodent models became increasingly acceptable surrogates to model the human heart either in vivo or through in vitro cultures. More recently, due to concerns regarding animal to human translation, including cross-species differences, the use of human iPSC-derived cardiomyocytes presented a renewed opportunity. Here, we conducted a comparative study, assessing cellular signaling through cardiac G protein-coupled receptors (GPCRs) in rat neonatal cardiomyocytes (RNCMs) and human induced pluripotent stem cell-derived cardiomyocytes. Genetically encoded biosensors were used to explore GPCR-mediated nuclear protein kinase A (PKA) and extracellular signal-regulated kinase 1/ 2 (ERK1/2) activities in both cardiomyocyte populations. To increase data granularity, a single-cell analytical approach was conducted. Using automated high content microscopy, our analyses of nuclear PKA and ERK1/2 signaling revealed distinct response clusters in rat and human cardiomyocytes. In line with this, bulk RNA-seq revealed key differences in the expression patterns of GPCRs, G proteins and downstream effector expression levels. Our study demonstrates that human stem cell-derived models of the cardiomyocyte offer distinct advantages for understanding cellular signaling in the heart.
Photocatalytic properties of BiFeO3 powders synthesized by the mixture of CTAB and Glycine
Authors: Asefi N. , Hasheminiasari M. , Masoudpanah S. M.
Published: July 2023

Highly pure BiFeO3 (BFO) powders were prepared by the solution combustion synthesis method using cetyltrimethylammonium bromide (CTAB) and glycine as fuels at various fuel-to-oxidant (φ) ratios. Microstructural characteristics, morphology, optical properties, and thermal analysis were studied by X-ray diffraction (XRD), scanning electron microscopy (SEM), diffuse reflectance spectroscopy (DRS), and differential thermal/thermogravimetry (DTA/TGA), respectively. The combusted powders prepared at different fuel content contained a small amount of impurity phases such as Bi24Fe2O39 and Bi2Fe4O9. During the calcination of BFO powders at 600 °C for 1 h, a nearly pure BFO phase was produced. Combusted powders photodegraded about 80% of methylene blue dye at φ = 2 through 90 min of visible light irradiation.
Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover
Authors: Burton-Smith Raymond N. , Song Chihong , Ueno Hiroshi , Murata Takeshi , Iino Ryota , Murata Kazuyoshi
Published: July 2023

The vacuolar-type ATPase from Enterococcus hirae (EhV-ATPase) is a thus-far unique adaptation of V-ATPases, as it performs Na+ transport and demonstrates an off-axis rotor assembly. Recent single molecule studies of the isolated V1 domain have indicated that there are subpauses within the three major states of the pseudo three-fold symmetric rotary enzyme. However, there was no structural evidence for these. Herein we activate the EhV-ATPase complex with ATP and identified multiple structures consisting of a total of six states of this complex by using cryo-electron microscopy. The orientations of the rotor complex during turnover, especially in the intermediates, are not as perfectly uniform as expected. The densities in the nucleotide binding pockets in the V1 domain indicate the different catalytic conditions for the six conformations. The off-axis rotor and its’ interactions with the stator a-subunit during rotation suggests that this non-uniform rotor rotation is performed through the entire complex.
Intermediate states in Andreev bound state fusion
Authors: Jünger Christian , Lehmann Sebastian , Dick Kimberly A. , Thelander Claes , Schönenberger Christian , Baumgartner Andreas
Published: July 2023

Hybridization is one of the most fundamental quantum mechanical phenomena, with the text book example of binding two hydrogen atoms in a hydrogen molecule. Here we report tunnel spectroscopy experiments illustrating the hybridization of another type of discrete quantum states, namely of superconducting subgap states that form in segments of a semiconducting nanowire in contact with superconducting reservoirs. We discuss a collection of intermediate states with unique (tunnel) spectroscopic fingerprints in the process of merging well-known individual bound states, hybridized by a central quantum dot and eventually coherently linking the reservoirs, carrying a Josephson current. These coupled and fused Andreev bound states can be seen as superconducting analogues to atomic and molecular single electron states in nature, and explain a variety of recent bound state spectra, with specific fingerprints that will have to be winnowed in future Majorana fusion experiments.
Environmental profile of the production of fragrance ingredients used in cosmetic products: comparative analysis of results obtained by life cycle assessment and the green chemistry-based eco-design tool GREEN MOTION™
Authors: Martz Patricia , Phan T. V. Tony , L'Haridon Jacques , Beausoleil Marie-Hélène , Lafaye Kévin , Gérand Yves , Gallardo Cyril
Published: July 2023

Environmental footprint evaluation of representative fragrance ingredients combining 2 complementary methodologies in the aim of providing a new method to eco-design fragrances, which are materials found in many commercialized daily-life products.
High wavenumber Raman spectroscopy for intraoperative assessment of breast tumour margins
Authors: Haskell Jennifer , Hubbard Thomas , Murray Claire , Gardner Benjamin , Ives Charlotte , Ferguson Douglas , Stone Nick
Published: July 2023

Spectra taken using a HWN Raman probe from bisected mastectomy specimens are capable of identifying tumour versus normal tissue through the difference in water, protein and lipid content.
An optical sensor for the sensitive determination of formaldehyde gas based on chromotropic acid and 4-aminoazobenzene immobilized in a hydrophilic membrane
Authors: Fernández-Ramos M. D. , Moraga-Cabezas A. , Medina-Castillo Antonio L. , Capitán-Vallvey L. F.
Published: July 2023

Optical sensor for highly selective determination of Formaldehyde gas at room temperature. Irreversible sensor with a detection limit lower than the maximum exposure concentrations recommended by both the WHO and OSHA. Good reproducibility and with only a 5-min response time.
Biodegradable covalent organic frameworks achieving tumor micro-environment responsive drug release and antitumor treatment
Authors: Li Tong , Wang Dianwei , Guo Zhaopei , Lin Lin , Meng Meng , Liu Cong , Hao Kai , Pang Xuan , Tian Huayu , Chen Xuesi
Published: July 2023

A biodegradable COF was constructed to break in response to tumors, enhancing the therapeutic effect on tumors.
Modification of insulating oils and oil-based titanium dioxide nanofluids for transformers: a review
Authors: Zhang Zilong , Zuo Fangmin , Cai Tianzi , Gai Xingyu , Wan Li , Lin Haidan , Wang Bolin , Zhang Haifeng
Published: July 2023

A timely comparison, analysis and summary of recent advances in the preparation, characterization, properties of different oil-based TiO2 NFs for transformers will contribute to provide a useful reference for such materials.
Improved electroluminescence efficiency derived from functionalized decoration of 1,3,4-oxadiazole (OXD)-based Ir(iii) complexes
Authors: Liu Jia-Wei , Tang Xiong , Liu Chang , Zhou Han-Chen , Wang Shi , Kang Hai-Peng , Li Yong-Hua , Lai Wen-Yong
Published: July 2023

Functionalized 1,3,4-oxadiazole (OXD) with electron-withdrawing properties was introduced into a fluorenyl OXD-based Ir(iii) complex, which exhibited enhanced device brightness and decreased efficiency roll-off.
The potential of near real-time monitoring of β-d-glucuronidase activity to establish effective warning systems in urban recreational waters
Authors: Makris Konstantinos F. , Hoefeijzers Bas , Seelen Laura , Schilperoort Remy , Langeveld Jeroen G.
Published: July 2023

The enzymatic activity of E. coli in urban surface waters gives a sharp but short response to contamination events.
Removal of toxic Microcystis aeruginosa using discharge plasma: mechanism and potential environmental risks
Authors: Gan Yanshun , Yan Pengcheng , Zhu Zhengfei , Zhang Rong , Qu Guangzhou , Wang Tiecheng , Li Ronghua , Zhang Zengqiang
Published: July 2023

Discharge plasma can remove Microcystis aeruginosa quickly and effectively, and reduce the potential environmental risk caused by the released DOM.
Hexavalent chromium waste removal via bioelectrochemical systems – a life cycle assessment perspective
Authors: Muazu Rukayya Ibrahim , Sadhukhan Jhuma , Venkata Mohan S. , Gadkari Siddharth
Published: July 2023

First LCA study for assessing the environmental performance of BESs for metal recovery application.
Influence of metal-coordinating comonomers on the coordination structure and binding in magnetic poly(ionic liquid)s
Authors: Foley Kayla , Condes Lucas , Walters Keisha B.
Published: July 2023

Metal coordinating comonomers influence the transition metal complex, polymer–metal ion binding, and subsequently material properties in magnetic responsive poly(ionic liquid)s.
Role of carboxylates in the phase determination of metal sulfide nanoparticles
Authors: Shults Andrey A. , Lu Guanyu , Caldwell Joshua D. , Macdonald Janet E.
Published: July 2023

At low carboxylate concentrations the sulfur source is highly reactive thiourea, which gives rise to sulfur rich nanoparticles. At high carboxylate concentrations, the sulfur source is the less reactive thiocyanate, resulting in sulfur poor phases.
A lysosome-targetable fluorescent probe for the ratiometric detection of formaldehyde in living cells and in vivo
Authors: Xu Hai , Xu Long-Zhou , Huai Rui-Ping , Zhang Chen , Suo Sheng-Nan , Li Ya-Qian , Peng Yu , Wang Ya-Wen
Published: July 2023

A new colorimetric and ratiometric fluorescent probe has been developed for formaldehyde based on the cyclization reaction.
A squaramide-based anionic hydrogen-bonded organic framework: enhancing sensing performance for pesticides by post-metallization with in situ imaging
Authors: Jiang Weiwei , Zheng Hao , Wu Yuxuan , Wu Pengyan , Jing Liyuan , Yuan Xingcheng , Wang Xichen , Wang Jian
Published: July 2023

A lanthanide modified squaramide-based anionic hydrogen-bonded organic framework could be used as a sensitive sensor for rapid detection of DCN pesticide residues in fresh produce nondestructively.
Machine learning of isomerization in porous molecular frameworks: exploring functional group pair distance distributions
Authors: Nurhuda Maryam , Hafidh Yusuf , Dogan Cansu , Packwood Daniel , Perry Carole C. , Addicoat Matthew A.
Published: July 2023

All possible isomers of a pore in a functionalized molecular framework material may be represented as a single histogram, showing the distances between functional groups.
Novel sensor for the determination of CA 15-3 in serum of breast cancer patients based on Fe–gallic acid complex doped in modified cellulose polymer thin films
Authors: AlGhamdi Hind A. , AlZahrani Yasmeen M. , Alharthi Salha , Mohy-Eldin Mohamed S. , Mohamed Ekram H. , Mahmoud Safwat A. , Attia Mohamed S.
Published: July 2023

Fe–gallic acid MOF embedded in an epoxy methyl cellulose polymer (CMC) thin film was synthesized and characterized by different micro-analytical tools such as: FE-SEM/EDX, XPS analysis, XRD analysis, FT-IR, and fluorescence spectroscopy.
Synthesis of centimeter-size two-dimensional hybrid perovskite single crystals with tunable, pure, and stable luminescence
Authors: Wang Haiyan , Wang Qiaohe , Ning Mengxin , Li Sen , Xue Renzhong , Chen Peng , Li Zijiong
Published: July 2023

The environment-friendly synthesis and property modulation of two-dimensional organic–inorganic halide perovskite (2D OHP) single crystals with large sizes and high quality are important for the fabrication of optoelectric devices.
Graphene nanowalls in photodetectors
Authors: Yang Jun , Yang Qi , Zhang Yongna , Wei Xingzhan , Shi Haofei
Published: July 2023

Graphene nanowalls (GNWs) have emerged as a promising material in the field of photodetection, thanks to their exceptional optical, electrical, mechanical, and thermodynamic properties.
Sustainable biosynthesized bimetallic ZnO@SeO nanoparticles from pomegranate peel extracts: antibacterial, antifungal and anticancer activities
Authors: Hashem Amr H. , Al-Askar Abdulaziz A. , Saeb Mohammad Reza , Abd-Elsalam Kamel A. , El-Hawary Ahmad S. , Hasanin Mohamed S.
Published: July 2023

Sustainable bimetallic nanoparticles (NPs) have attracted particular attention in the past decade.
Detail computational study about the structural, electronic, optical, and mechanical properties of RbVX3 (Cl, Br, I) halide perovskite materials
Authors: Khan Naimat Ullah , Iqbal Javed , Abdullah  , Algahtani Ali , Al-Humaidi Jehan Y. , Tirth Vineet , Safeen Kashif , Alsuhaibani Amnah Mohammed , Al-Mughanam Tawfiq , Refat Moamen S. , Zaman Abid
Published: July 2023

The non-toxic nature of lead-free materials with cubic perovskite structure has attracted the researcher's attention, and huge work is ongoing for the search of such materials.
Mesoporous sol–gel silica supported vanadium oxide as effective catalysts in oxidative dehydrogenation of propane to propylene
Authors: Guo Xin-Peng , Wang Xiao-Xiao , LU Hai-Qiang , Liu Zhen-Min
Published: July 2023

Strongly-interacted [OV(OSi)3] sites with mesoporous silica, triggered by a surfactant-modified sol–gel method, promotes the selective oxidative dehydrogenation of propane to propylene.
Ecotoxicological significance of bio-corona formation on micro/nanoplastics in aquatic organisms
Authors: Rex M Camil , Debroy Abhrajit , Nirmala M. Joyce , Mukherjee Amitava
Published: July 2023

Biocorona formation on M/NPs potentially impacts the eco-toxicity and fate and transport of M/NPs in the aquatic environment.
Mild and efficient synthesis of benzothiazolopyrimidine derivativesviaCuAAC/ring cleavage/cyclization reaction
Authors: Yang Weiguang , Luo Danyang , Li Guanrong , Hu Weigao , Zheng Jia , Chen Lanmei
Published: July 2023

Three-component one-pot synthesis of benzothiazolopyrimidine derivatives through a CuAAC/ring cleavage/cyclization process.
Advances in in situ and in-line liquid–liquid extraction for bioprocess intensification
Authors: Chaichol Patchara , Weeranoppanant Nopphon
Published: July 2023

In situ extraction takes place inside a fermenter or bioreactor, while in-line extraction is operated in another external unit. Both modes can be used to remove inhibitory species and enhance productivity in certain bioprocesses.
The structure of disintegrating defect clusters in smectic C freely suspended films
Authors: Stannarius Ralf , Harth Kirsten
Published: July 2023

The spontaneous selection of symmetric defect configurations after disintegration of high-strength defects or defect clusters is explained by simple geometrical arguments. The images show experimental (left) and calculated (right) snapshots.
Ultra-high initial coulombic efficiency of the TiO2 anode induced by the synergistic role of the electrolyte and binder for sodium-ion batteries
Authors: Yang Li , Yang Yingchang , Shi Wei , Leng Senlin , Cheng Deliang , Hou Hongshuai
Published: July 2023

TiO2 nanocrystallites (5–8 nm) fabricated by an electrochemical method with CMC binder and ether-based electrolyte present a high initial coulombic efficiency (81.6%) and a high specific capacity of 166.23 mA h g−1 after 4800 cycles at 2C for SIBs.
Controlling the metal–support interaction with steam-modified ceria to boost Pd activity towards low-temperature CO oxidation
Authors: An Yuanyuan , Chen Sheng-Yu , Wang Beibei , Zhou Li , Hao Guoxiu , Wang Yanli , Chen Junchen , Tsung Chia-Kuang , Liu Zhi , Chou Lien-Yang
Published: July 2023

OH-modified ceria can promote Pd dispersion and weaken CO adsorption strength to greatly improve the low-temperature CO oxidation activity.
Direct monitoring of the potassium charge carrier in Prussian blue cathodes using potassium K-edge X-ray absorption spectroscopy
Authors: Mayer Alexander J. , Beynon Owain T. , Logsdail Andrew J. , Wijayantha K. G. Upul , Dann Sandra E. , Marco José F. , Elliott Joshua D. , Aramini Matteo , Cibin Giannantonio , Kondrat Simon A.
Published: July 2023

Monitoring the evolution of potassium carriers during the charge/discharge of Prussian blue batteries by operando potassium K-edge X-ray absorption spectroscopy.
Synergy of the transmittance fluctuation factor and absorption selectivity for efficient semitransparent perovskite/organic tandem solar cells with high color-fidelity
Authors: Bai Yiming , Han Fei , Zeng Rui , Tian Shilei , Wang Fuzhi , Wang Xi , Dai Meilin , Li Meicheng , Tan Zhan'ao
Published: July 2023

A numerical method which combines EQEff, TFF and absorption selectivity, is established to design semitransparent perovskite/organic TSCs with outstanding color-fidelity.
Fireproof ultrastrong all-natural cellulose nanofiber/montmorillonite-supported MXene nanocomposites with electromagnetic interference shielding and thermal management multifunctional applications
Authors: Cheng Rui , Wu Ying , Wang Bin , Zeng Jinsong , Li Jinpeng , Xu Jun , Gao Wenhua , Chen Kefu
Published: July 2023

A Janus structural engineering strategy is proposed to prepare fireproof and high-strength MXene-based electromagnetic interference (EMI) shielding papers supported by all-natural CNFs and MMT.
Trap-regulated highly efficient mechanoluminescence and persistent mechanoluminescence of Ca2MgSi2O7:Eu2+
Authors: Yan Yingjuan , Fang Shaofan , Li Yanyan , Xu Yixuan , Song Yingdan , Ma Zhidong , Shi Yaru , Zhao Lei , Wang Zhaofeng
Published: July 2023

Mechanoluminescence and persistent mechanoluminescence were achieved in Ca2MgSi2O7:Eu2+/epoxy composites, and were enhanced simultaneously by utilizing a trap-regulation strategy.
Joint experimental and theoretical study of PbGa2S4 under compression
Authors: Garcia-Sanchez Tania , Gallego-Parra Samuel , Liang Akun , Rodrigo-Ramon José Luis , Muñoz Alfonso , Rodriguez-Hernandez Plácida , Gonzalez-Platas Javier , Sans Juán Ángel , Cuenca-Gotor Vanesa Paula , Osman Hussien H. , Popescu Catalin , Ursaki Veaceslav , Tiginyanu Ion M. , Errandonea Daniel , Manjón Francisco Javier
Published: July 2023

The effect of pressure on the structural, vibrational, and optical properties of lead thiogallate, PbGa2S4, crystallizing under room conditions in the orthorhombic EuGa2S4-type structure (space group Fddd), is investigated.
Recast Therapy for Treating Syntax in Bilingual Children With Developmental Language Disorder: A Feasibility and Early Efficacy Study Examining the Role of Language of Intervention on Outcomes
Authors: Castilla-Earls Anny , Owen Van Horne Amanda
Published: July 2023

Purpose:

The purpose of this study is to examine the feasibility of the delivery of complex syntax recast therapy via telepractice to Spanish–English bilingual children and provide preliminary evidence of the efficacy of this approach.

Method:

Fifteen bilingual children with developmental language disorders were stratified based on language proficiency and randomized to one of three treatment conditions: Spanish only (
n
= 5), English only (
n
= 6), or Spanish + English (
n
= 4). Using a within-subject design, we hypothesized that we could document treatment efficacy based on change in the treated structure in the absence of change in an untreated comparison structure. All 15 children completed ~16 hr of treatment via telepractice and participated in pre- and posttesting of their production of conditional adverbs (treated structure) and subject relative clauses (untreated structure) carried out by a masked assessor.

Results:

Analyses included all participants. Treatment fidelity was high, and participant attendance was remarkable, indicating feasibility. Regarding efficacy, recast therapy led to group-level gains on treated syntactic structures that exceeded those observed for the untreated comparison structure. For the 11 children who received therapy in only one language, approximately equal gains were observed in both the treated and untreated languages for conditional adverbials.

Conclusions:

Preliminary evidence suggests that for highly overlapping structures like conditional adverbials, recast therapy is effective and leads to change in both of the child's languages. Larger studies are required to understand how language of administration and proficiency may affect outcomes.

Supplemental Material:

https://doi.org/10.23641/asha.23739996