bluuebunny/crossref_metadata_2025 huggingface dataset:
Teaching Theory and Space: Human Territoriality in Political Science
Authors: Tagma Halit M.E.
Published: October 2021

This article describes a technique that helps students to understand how a theory about human characteristics may impact behavior. I use a mini-simulation in which two volunteers engage in small talk, I point out that they engage with one another at a certain distance and angle that reflects social space. As the exercise progresses, students easily relate to the theory of human territoriality, which is defined as the symbolic and physical connection to a space considered as their own. This mini-simulation achieves the following learning objectives: understanding (1) that theories are relevant and help to explain human behavior; (2) the workings of the individual level of analysis; and (3) that theories are not universal and have limits to their application across culture, time, and space. This teaching technique does not require preparation time or resources, and students easily comprehend the expected learning outcomes.
CREENCIAS BÁSICAS SOBRE LOS OTROS, EL YO Y EL MUNDO, EN EMERGENCIA SANITARIA POR COVID-19
Authors:   Universidad Central del Ecuador, Marcillo Cecilia del Carmen
Published: October 2021

El presente estudio habla sobre las creencias básicas que manejan las personas frente a la situación de emergencia sanitaria por covid-19, mismas que están implícitas en el pensamiento de cada una. El objetivo general fue comparar las respuestas sobre las creencias que las personas manejan sobre el yo, el mundo y los otros frente a la situación de emergencia sanitaria por corona virus, en dos momentos diferentes de la pandemia, lo que permite contar con nuevo conocimiento sobre el comportamiento humano en situaciones de alto riesgo para su vida y su adaptación a las mismas. El estudio es comparativo, de diseño no experimental longitudinal, de enfoque cuantitativo; para obtener la información se aplicó en dos instantes diferentes la escala impact on beliefs questionnaire de corsini (2004) a 150 personas voluntarias, de manera individual y en línea. Se concluye que las personas en esta emergencia sanitaria han disminuido considerablemente en el segundo momento sus creencias positivas en relación a sí mismas, al mundo y hacia los otros, justificadas desde la desconfianza misma de contagio por el peligro de muerte que esta situación implica, sin embargo, no se sienten descorazonadas o desmoralizadas.
Orthokeratology Lenses for Myopia Control. Factors Affecting Efficacy (Literature Review)
Authors: Zhabina O. A. , Andrienko G. V.
Published: October 2021

This review focuses on the major factors affecting the efficacy of myopia control in patients wearing orthokeratology lenses (OKLs). Orthokeratology lenses have been proven to be effective for myopia control, however further efficacy comparisons between OKLs of standard and custom designs revealed that customization of parameters resulted in an increase of relative peripheral myopia in myopic patients regardless of their initial refractive error. Purpose: to systematize the data of the literature on the effectiveness of orthoceratological correction to control the progression of myopia. Methods. The analysis of publications on the resources PubMed, eLibrary, Cochrane, Cyberleninka is carried out. Results. According to the search results, the main factor saffecting the degree of control of myopia in patients using orthokeratological correction were highlighted. Conclusions. Evaluation of the anatomical and physiological parameters of patients, as well as development of customizable designs of OKLs for myopia control will allow for greater control over myopia progression in its early stages.
Cosmetics and Contact Lenses: Influence on Safety
Authors: Zimovets S. V. , Sverdlik A. Ya.
Published: October 2021

The article describes the effect of decorative cosmetics on the material of soft contact lenses and studies specific features that can be observed in SCL wearers who use cosmetics extensively. Examples of complications associated with the use of various cosmetic products that may occur in contact lens wearers are given. Conclusion: in cases when patients have complaints about discomfort and dry eyes, doctors should pay attention to the amount of decorative cosmetics used as well as study the effect it can have on the surface of the contact lenses.
Suyya’s Flood: Numerical Models of Kashmir’s Medieval Megaflood and Ancient Lake Kerewa Drainage Events
Authors: Urooj Muntaha , Bilham Roger , Bali Bikram S. , Ahmed S. Imran
Published: October 2021

In the mid-ninth century, an earthquake triggered a landslide that blocked the narrow gorge of the Jhelum River where it exits the Kashmir Valley. The landslide impounded a lake that extended ≈100 km along the floor of the valley, implying an impounded volume of ≤21 km
3
, flooding the capital, Srinagar, and much agricultural land. An engineered breach of the landslide was contrived by a Medieval engineer resulting in the catastrophic release of flood waters. Using reasonable assumptions we calculate the probable minimum drainage time of this Medieval flood (<4 days) and maximum downstream surge velocities (≈12 m/s). These would have been sufficient to transport boulders in the bed of the Jhelum with dimensions of ≈6 m, consistent with those currently present in some reaches of the river. Given the morphology of the Jhelum gorge we consider that landslide outburst floods may have been common in Kashmir’s history. Ancient shorelines indicate that paleo-lake volumes in the Kashmir Valley may have exceeded 400 km
3
which, were they released in catastrophic floods, would have been associated with potential downstream outburst velocities >32 m/s, able to transport boulders with dimensions ≈40 m, far in excess of any found in the course of the Jhelum or in the Punjab plains. Their absence suggests that Kashmir’s ancient lakes were not lowered by outburst mechanisms much exceeding those associated with Suyya’s flood. Present-day floods have been many tens of meters shallower than those impounded by landslides in the Jhelum in the past several thousands of years. A challenge for future study will be to date Kashmir’s ancient shorelines to learn how often landslides and major impoundment events may have occurred in the valley.
Regional Exploration and Characterisation of CO
2
Storage Prospects in the Utsira-Skade Aquifer, North Viking Graben, North Sea
Authors: Lloyd C. , Huuse M. , Barrett B. J. , Newton A. M. W.
Published: October 2021

Subsurface CO
2
storage is considered a key element of reducing anthropogenic emissions in virtually all scenarios compatible with limiting global warming to 1.5°C. The Utsira-Skade Aquifer (Utsira, Eir and Skade Formations), northern North Sea, has been identified as a suitable reservoir. Although the overall storage capacity of the full aquifer has been estimated based on regional data, it is lacking an integrated assessment of containment and internal heterogeneity, to identify optimal areas for injection and for calculation of site-specific storage capacities. A high-resolution, broadband 3D seismic reflection dataset, full waveform inverted velocity data and 102 exploration wells are utilised to provide a catalogue of CO
2
storage prospects in the northern Utsira-Skade Aquifer. This is achieved through: 1) definition of the aquifer’s spatial limits; 2) calculation of porosity distribution; 3) assessment of the extent, geomorphology, thickness variability, and containment confidence (CC) of mudstones; and 4) mapping of closures through fill-to-spill simulations. CO
2
storage capacity was calculated for the prospects using two approaches; using the full reservoir thickness (FRT) beneath the closures and using only the thickness from the closure top to the spill point (TSP), i.e., within structural traps. Porosity ranges from 29 to 39% across the aquifer and is higher in the Utsira and Eir Fms. relative to the underlying Skade Fm. The mudstone separating the Skade and Eir/Utsira Fm. has a thickness > 50 m, and is a potential barrier for CO
2
. Other intra-aquifer mudstones were mainly interpreted to act as baffles to flow. Structural traps at the top Utsira and Skade Fms. yield fifteen prospects, with criteria of > 700 m depth and FRT storage capacity of > 5 Mt CO
2
. They have a combined storage capacity of 330 Mt CO
2
(FRT) or 196 Mt CO
2
(TSP). Five prospects have a positive CC score (total capacity: 54 Mt CO
2
FRT or 39 Mt CO
2
TSP). Additional storage capacity could be achieved through more detailed analysis of the seal to upgrade the CC scores, or through use of a network of the mapped closures with a fill-to-spill approach, utilising more of the aquifer.
Potent Anti-Inflammatory Effects of Tetracyclines on Human Eosinophils
Authors: Gehring Manuela , Wieczorek Dorothea , Kapp Alexander , Wedi Bettina
Published: October 2021

Eosinophils are potent pro-inflammatory cells. Not only in allergic diseases but also in other diseases there is a need for treatment strategies to induce resolution of eosinophil-mediated inflammation. During the last years beneficial non-antibiotic activities of tetracyclines (TCNs) have been shown in different diseases in which eosinophils play a role, for example, asthma and bullous pemphigoid. The working mechanism of these effects remains to be clarified. Aim of the present study was to investigate the effects of TCNs on eosinophils. Flow cytometry analysis of apoptosis, mitochondrial membrane potential, activation of caspases, intracellular H2O2and calcium, surface expression of eosinophil activation markers was performed in highly purified peripheral blood eosinophils of non-atopic donors. Tetracycline hydrochloride, minocycline and doxycycline significantly induced eosinophil apoptosis. All TCNs were able to significantly overcome the strong survival enhancing effects of pro-eosinophilic cytokines and staphylococcus aureus enterotoxins. Tetracycline hydrochloride induced eosinophil apoptosis was accompanied by intracellular production of hydrogen peroxide, loss of mitochondrial membrane potential and activation of caspases. Moreover, tetracycline hydrochloride significantly down regulated eosinophil surface expression of CD9 and CD45, and of the activation markers CD11b and CD69, but not of CD54, CD63, or CD95. Our data, propably for the first time, point to a potent anti-inflammatory role of TCNs on eosinophils.
Rho Signaling in Synaptic Plasticity, Memory, and Brain Disorders
Authors: Zhang Haorui , Ben Zablah Youssif , Zhang Haiwang , Jia Zhengping
Published: October 2021

Memory impairments are associated with many brain disorders such as autism, Alzheimer’s disease, and depression. Forming memories involves modifications of synaptic transmission and spine morphology. The Rho family small GTPases are key regulators of synaptic plasticity by affecting various downstream molecules to remodel the actin cytoskeleton. In this paper, we will review recent studies on the roles of Rho proteins in the regulation of hippocampal long-term potentiation (LTP) and long-term depression (LTD), the most extensively studied forms of synaptic plasticity widely regarded as cellular mechanisms for learning and memory. We will also discuss the involvement of Rho signaling in spine morphology, the structural basis of synaptic plasticity and memory formation. Finally, we will review the association between brain disorders and abnormalities of Rho function. It is expected that studying Rho signaling at the synapse will contribute to the understanding of how memory is formed and disrupted in diseases.
In vivo Regeneration of Ganglion Cells for Vision Restoration in Mammalian Retinas
Authors: Xiao Dongchang , Jin Kangxin , Qiu Suo , Lei Qiannan , Huang Wanjing , Chen Haiqiao , Su Jing , Xu Qiang , Xu Zihui , Gou Bin , Tie Xiaoxiu , Liu Feng , Liu Sheng , Liu Yizhi , Xiang Mengqing
Published: October 2021

Glaucoma and other optic neuropathies affect millions of people worldwide, ultimately causing progressive and irreversible degeneration of retinal ganglion cells (RGCs) and blindness. Previous research into cell replacement therapy of these neurodegenerative diseases has been stalled due to the incapability for grafted RGCs to integrate into the retina and project properly along the long visual pathway. In vivo RGC regeneration would be a promising alternative approach but mammalian retinas lack regenerative capacity. It therefore has long been a great challenge to regenerate functional and properly projecting RGCs for vision restoration in mammals. Here we show that the transcription factors (TFs) Math5 and Brn3b together are able to reprogram mature mouse Müller glia (MG) into RGCs. The reprogrammed RGCs extend long axons that make appropriate intra-retinal and extra-retinal projections through the entire visual pathway to innervate both image-forming and non-image-forming brain targets. They exhibit typical neuronal electrophysiological properties and improve visual responses in RGC loss mouse models. Together, our data provide evidence that mammalian MG can be reprogrammed by defined TFs to achieve in vivo regeneration of functional RGCs as well as a promising new therapeutic approach to restore vision to patients with glaucoma and other optic neuropathies.
A Three-Dimensional Electrospun Li6.4La3Zr1.4Ta0.6O12–Poly (Vinylidene Fluoride-Hexafluoropropylene) Gel Polymer Electrolyte for Rechargeable Solid-State Lithium Ion Batteries
Authors: Wang Donghuang , Cai Dan , Zhong Yu , Jiang Zhao , Zhang Shengzhao , Xia Xinhui , Wang Xiuli , Tu Jinagping
Published: October 2021

Developing high-quality solid-state electrolytes is important for producing next-generation safe and stable solid-state lithium-ion batteries. Herein, a three-dimensional highly porous polymer electrolyte based on poly (vinylidenefluoride-hexafluoropropylene) (PVDF-HFP) with Li6.4La3Zr1.4Ta0.6O12 (LLZTO) nanoparticle fillers (PVDF-HFP-LLZTO) is prepared using the electrospinning technique. The PVDF-HFP-LLZTO gel polymer electrolyte possesses a high ionic conductivity of 9.44 × 10–4 S cm−1 and a Li-ion transference number of 0.66, which can be ascribed that the 3D hierarchical nanostructure with abundant porosity promotes the liquid electrolyte uptake and wetting, and LLZTO nanoparticles fillers decrease the crystallinity of PVDF-HFP. Thus, the solid-state lithium battery with LiFePO4 cathode, PVDF-HFP-LLZTO electrolyte, and Li metal anode exhibits enhanced electrochemical performance with improved cycling stability.
Zeolite-Based Materials for the Catalytic Oxidation of VOCs: A Mini Review
Authors: Xue Tianshan , Yang Li
Published: October 2021

Catalysts for VOCs combustion have been widely studied and zeolite-based materials and have been structured to meet the need of particle use in this field. This review summarized several new trends in zeolite-based catalysts for VOCs catalytic oxidation. Intensive effort has been devoted to the optimization of composition and structure of catalysts, abatement of CVOCs, design of zeolite-based monolithic catalysts and adsorbent/catalyst bi-functional material. The suggestions for further work here presented are put forward based on the collation of recently published papers.
Chimney Graft Technique Combined With Embolization for Treating Ruptured Aortic Arch Lesions
Authors: Bao Xianhao , Zhao Yuxi , Li Tao , Wu Mingwei , Zeng Zhaoxiang , Gao Minxin , Xu Ding , Feng Jiaxuan , Feng Rui
Published: October 2021

Background: This study aimed to share the experience in applying the chimney graft technique combined with embolization for treating aortic arch rupture under emergency conditions and evaluating early-term results in these patients.

Methods: This study retrospectively included patients with ruptured aortic arch lesions who received the chimney graft technique combined with embolization between March 2016 and March 2021. The primary endpoint was a technical success, deemed as successful stent graft deployment to the planned location, patency of the target branch vessel, and absence of significant type I endoleak. The secondary endpoint was clinical success defined with the size of false lumen in follow-up remaining unchanged or decreasing over time, 30-day mortality, complication, and primary patency of chimney graft.

Results: This study included 12 patients (age, 61 ± 12 years; male, 83%). Five patients (42%) received single chimney, one patient (8%) received double chimney, and six patients (50%) received triple chimney. Intraoperative type I endoleak occurred in six patients (50%) who underwent endovascular embolization in the primary operation. Post-operative type I endoleak, evaluated by computed tomography angiography examination following the primary operation, occurred in seven patients (58%), including one patient who received endovascular embolization two times. All patients with post-operative type I endoleak were successfully re-treated using coil and Onyx glue within 1 week, and the median length of stay was 22 ± 11 days (range: 7–44 days). Overall technical success was 100%. Eleven patients had completed their follow-up (median, 12 months, range: 1–34 months), and one patient was out of contact. The 30-day mortality was 9% (1/11, post-operative death of a patient with cerebral hemorrhage). No major complications and no chimney compression, migration, occlusion, or stenosis were recorded during follow-up. Seven patients (58%) have ≥6 months of clinical follow-up time with appropriate imaging. In four (57%) of these patients, diameter stabilization was detected, whereas three (43%) experienced significant reduction (≥5 mm).

Conclusion: The patients in this study had satisfactory early-term outcomes. The chimney graft technique combined with coil and Onyx glue embolization may be a safe and effective treatment for ruptured aortic arch lesions under emergency conditions.
Inflammation in Metabolic Cardiomyopathy
Authors: Wenzl Florian A. , Ambrosini Samuele , Mohammed Shafeeq A. , Kraler Simon , Lüscher Thomas F. , Costantino Sarah , Paneni Francesco
Published: October 2021

Overlapping pandemics of lifestyle-related diseases pose a substantial threat to cardiovascular health. Apart from coronary artery disease, metabolic disturbances linked to obesity, insulin resistance and diabetes directly compromise myocardial structure and function through independent and shared mechanisms heavily involving inflammatory signals. Accumulating evidence indicates that metabolic dysregulation causes systemic inflammation, which in turn aggravates cardiovascular disease. Indeed, elevated systemic levels of pro-inflammatory cytokines and metabolic substrates induce an inflammatory state in different cardiac cells and lead to subcellular alterations thereby promoting maladaptive myocardial remodeling. At the cellular level, inflammation-induced oxidative stress, mitochondrial dysfunction, impaired calcium handling, and lipotoxicity contribute to cardiomyocyte hypertrophy and dysfunction, extracellular matrix accumulation and microvascular disease. In cardiometabolic patients, myocardial inflammation is maintained by innate immune cell activation mediated by pattern recognition receptors such as Toll-like receptor 4 (TLR4) and downstream activation of the NLRP3 inflammasome and NF-κB-dependent pathways. Chronic low-grade inflammation progressively alters metabolic processes in the heart, leading to a metabolic cardiomyopathy (MC) phenotype and eventually to heart failure with preserved ejection fraction (HFpEF). In accordance with preclinical data, observational studies consistently showed increased inflammatory markers and cardiometabolic features in patients with HFpEF. Future treatment approaches of MC may target inflammatory mediators as they are closely intertwined with cardiac nutrient metabolism. Here, we review current evidence on inflammatory processes involved in the development of MC and provide an overview of nutrient and cytokine-driven pro-inflammatory effects stratified by cell type.
The Association Between Serum/Plasma Leptin Levels and Obstructive Sleep Apnea Syndrome: A Meta-Analysis and Meta-Regression
Authors: Li Xiaoyan , He Jie
Published: October 2021

Background

Obstructive sleep apnea syndrome (OSAS) is associated with various adipokines. Leptin, a common adipokine, has attracted considerable attention of many researchers in recent years. So far, there has been little agreement on whether blood leptin levels differ in patients with OSAS. Thus, this meta-analysis examined the relationship between serum/plasma leptin levels and the occurrence of OSAS.

Method

WanFang, Embase, CNKI, Medline, SinoMed, Web of Science, and PubMed were searched for articles before March 30, 2021, with no language limitations. STATA version 11.0 and R software version 3.6.1 were used to analyze the obtained data. The weighted mean difference and correlation coefficients were used as the main effect sizes with a random-effects model and a fixed-effects model, respectively. Trial sequential analysis was conducted using dedicated software.

Result

Screening of 34 publications identified 45 studies that met the inclusion criteria of this meta-analysis and meta-regression. Our results suggested that plasma/serum leptin levels were remarkably higher in individuals with OSAS than in healthy individuals. Subgroup analyses were performed based on OSAS severity, ethnicity, age, body mass index, assay type, and sample source. The serum and plasma leptin levels were increased in nearly all OSAS subgroups compared to those in the corresponding control groups. Meta-regression analysis indicated that age, BMI, severity, assay approaches, study design, PSG type and ethnicity did not have independent effect on leptin levels. Furthermore, a positive relationship between the serum/plasma leptin level and apnea-hypopnea index (AHI) was found in the meta-analysis. The results of the trial sequential analysis suggested that the enrolled studies surpassed the required information size, confirming that our study findings were reliable.

Conclusion

Our study results demonstrate that OSAS patients have higher leptin levels in serum/plasma compared to controls, and the serum/plasma leptin level is positively correlated with AHI, especially in adults.
Myoglobin Protects Breast Cancer Cells Due to Its ROS and NO Scavenging Properties
Authors: Quinting Theresa , Heymann Anna Katharina , Bicker Anne , Nauth Theresa , Bernardini Andre , Hankeln Thomas , Fandrey Joachim , Schreiber Timm
Published: October 2021

Myoglobin (MB) is an oxygen-binding protein usually found in cardiac myocytes and skeletal muscle fibers. It may function as a temporary storage and transport protein for O2 but could also have scavenging capacity for reactive oxygen and nitrogen species. In addition, MB has recently been identified as a hallmark in luminal breast cancer and was shown to be robustly induced under hypoxia. Cellular responses to hypoxia are regulated by the transcription factor hypoxia-inducible factor (HIF). For exploring the function of MB in breast cancer, we employed the human cell line MDA-MB-468. Cells were grown in monolayer or as 3D multicellular spheroids, which mimic the in vivo avascular tumor architecture and physiology with a heterogeneous cell population of proliferating cells in the rim and non-cycling or necrotic cells in the core region. This central necrosis was increased after MB knockdown, indicating a role for MB in hypoxic tumor regions. In addition, MB knockdown caused higher levels of HIF-1α protein after treatment with NO, which also plays an important role in cancer cell survival. MB knockdown also led to higher reactive oxygen species (ROS) levels in the cells after treatment with H2O2. To further explore the role of MB in cell survival, we performed RNA-Seq after MB knockdown and NO treatment. 1029 differentially expressed genes (DEGs), including 45 potential HIF-1 target genes, were annotated in regulatory pathways that modulate cellular function and maintenance, cell death and survival, and carbohydrate metabolism. Of these target genes, TMEFF1, TREX2, GLUT-1, MKNK-1, and RAB8B were significantly altered. Consistently, a decreased expression of GLUT-1, MKNK-1, and RAB8B after MB knockdown was confirmed by qPCR. All three genes of interest are often up regulated in cancer and correlate with a poor clinical outcome. Thus, our data indicate that myoglobin might influence the survival of breast cancer cells, possibly due to its ROS and NO scavenging properties and could be a valuable target for cancer therapy.
Limnochemistry and Plankton Diversity in Some High Altitude Lakes of Kashmir Himalaya
Authors: Islam Sheikh Tajamul , Dar Shahid Ahmad , Sofi Mohd Sharjeel , Bhat Sami Ullah , Sabha Inam , Hamid Aadil , Jehangir Arshid , Bhat Aftab Ahmad
Published: October 2021

High altitude lakes (HALs) of Kashmir Himalaya are the important ecosystems in the mountain ecology of the broader Hindukush Himalayan region. This article provides a comprehensive information about the plankton (phytoplankton and periphyton) assemblages, water quality (WQ), bathymetry, morphometry, and land use land cover (LULC) of some select high altitude mountain lakes of Kashmir Himalaya. LULC analysis revealed that the catchment of the lakes spread over an area of about 16179 ha, is covered by different land cover types dominated by pastures (50.8%), followed by barren rocky (32.6%), snow and glaciers (11.9%), lakes (2.5%), forest (2%), and streams (0.2%). Bathymetric and morphometric analysis revealed that the Gangbal Lake is the deepest (84 m) and largest (162.4 ha) among the investigated lakes. The water quality index revealed that all the HALs have the excellent water quality category. Statistical analysis (Wilk’s λ) depicted that nitrate-nitrogen (NO3−-N), nitrite nitrogen (NO2−-N), ammoniacal nitrogen (NH3-N), total phosphorus (TP), and magnesium hardness (Mg-H) are responsible for major variability between all HALs sites. The cations followed the order of Ca2+> Mg2+> Na+> K+while as anions followed the order as HCO3−> Cl−> SO42−. Algal composition (phytoplankton and periphyton) assessment revealed the presence of 61 taxa belonging to Bacillariophyceae (45), Chlorophyceae (14), Cyanophyceae (1), and Xanthophyceae (1). The higher dominance of Bacillariophyceae indicates oligotrophic nature of the lakes. Canonical correspondence analysis (CCA) highlighted the role of various water quality parameters like pH, EC, and TDS on the composition of phytoplankton and periphyton species among the lakes. The present study therefore generated a baseline database for some of the HALs of Kashmir Himalaya that can act as a precursor for more research on future changes in the lake ecosystems of the region.
Can Industrial Agglomeration Facilitate Green Development? Evidence From China
Authors: Xie WanCheng , Li Xing
Published: October 2021

Industrial agglomeration can promote economic growth through knowledge spillover and cooperation, while it may also bring serious pollution problems. Therefore, clarifying the relationship between industrial agglomeration and green development is of great significance to the realization of China’s sustainable development. In order to study the causal relationship between industrial agglomeration and green development, this paper first adopts the Overall Malquist Index method to more accurately measure the green development of Chinese cities characterized by environmental total factor productivity (ETFP). Subsequently, this paper uses the panel data of prefecture-level cities from 2003 to 2016 to study the causal relationship between industrial agglomeration and green development. We found that China’s current industrial agglomeration can promote green development. Mechanism analysis shows that industrial agglomeration can promote green development through improving the technological innovation, strengthening government intervention and optimizing the industrial structure. Finally, heterogeneity analysis shows that industrial agglomeration has a stronger role in promoting green development in areas where the economy is more developed, the degree of resource dependence is lower, and the degree of industrial agglomeration is higher. This paper not only provides theoretical and policy references for the research on industrial agglomeration and green development, but also provides experience reference for other developing countries.
Assessing Pathways of Climate Change Effects in SpaDES: An Application to Boreal Landbirds of Northwest Territories Canada
Authors: Micheletti Tatiane , Stewart Frances E. C. , Cumming Steven G. , Haché Samuel , Stralberg Diana , Tremblay Junior A. , Barros Ceres , Eddy Ian M. S. , Chubaty Alex M. , Leblond Mathieu , Pankratz Rhiannon F. , Mahon C. L. , Van Wilgenburg Steven L. , Bayne Erin M. , Schmiegelow Fiona , McIntire Eliot J. B.
Published: October 2021

Distributions of landbirds in Canadian northern forests are expected to be affected by climate change, but it remains unclear which pathways are responsible for projected climate effects. Determining whether climate change acts indirectly through changing fire regimes and/or vegetation dynamics, or directly through changes in climatic suitability may allow land managers to address negative trajectories via forest management. We used SpaDES, a novel toolkit built in R that facilitates the implementation of simulation models from different areas of knowledge to develop a simulation experiment for a study area comprising 50 million ha in the Northwest Territories, Canada. Our factorial experiment was designed to contrast climate effects pathways on 64 landbird species using climate-sensitive and non-climate sensitive models for tree growth and mortality, wildfire, and landbirds. Climate-change effects were predicted to increase suitable habitat for 73% of species, resulting in average net gain of 7.49 million ha across species. We observed higher species turnover in the northeastern, south-central (species loss), and western regions (species gain). Importantly, we found that most of the predicted differences in net area of occupancy across models were attributed to direct climate effects rather than simulated vegetation change, despite a similar relative importance of vegetation and climate variables in landbird models. Even with close to a doubling of annual area burned by 2100, and a 600 kg/ha increase in aboveground tree biomass predicted in this region, differences in landbird net occupancy across models attributed to climate-driven forest growth were very small, likely resulting from differences in the pace of vegetation and climate changes, or vegetation lags. The effect of vegetation lags (i.e., differences from climatic equilibrium) varied across species, resulting in a wide range of changes in landbird distribution, and consequently predicted occupancy, due to climate effects. These findings suggest that hybrid approaches using statistical models and landscape simulation tools could improve wildlife forecasts when future uncoupling of vegetation and climate is anticipated. This study lays some of the methodological groundwork for ecological adaptive management using the new platform SpaDES, which allows for iterative forecasting, mixing of modeling paradigms, and tightening connections between data, parameterization, and simulation.
Interdependence of Thyroid and Corticosteroid Signaling in Vertebrate Developmental Transitions
Authors: Rousseau Karine , Dufour Sylvie , Sachs Laurent M.
Published: October 2021

Post-embryonic acute developmental processes mainly allow the transition from one life stage in a specific ecological niche to the next life stage in a different ecological niche. Metamorphosis, an emblematic type of these post-embryonic developmental processes, has occurred repeatedly and independently in various phylogenetic groups throughout metazoan evolution, such as in cnidarian, insects, molluscs, tunicates, or vertebrates. This review will focus on metamorphoses and developmental transitions in vertebrates, including typical larval metamorphosis in anuran amphibians, larval and secondary metamorphoses in teleost fishes, egg hatching in sauropsids and birth in mammals. Two neuroendocrine axes, the hypothalamic-pituitary-thyroid and the hypothalamic-pituitary-adrenal/interrenal axes, are central players in the regulation of these life transitions. The review will address the molecular and functional evolution of these axes and their interactions. Mechanisms of integration of internal and environmental cues, and activation of these neuroendocrine axes represent key questions in an “eco-evo-devo” perspective of metamorphosis. The roles played by developmental transitions in the innovation, adaptation, and plasticity of life cycles throughout vertebrates will be discussed. In the current context of global climate change and habitat destruction, the review will also address the impact of environmental factors, such as global warming and endocrine disruptors on hypothalamic-pituitary-thyroid and hypothalamic-pituitary-adrenal/interrenal axes, and regulation of developmental transitions.
Joint Analysis of Genetic Correlation, Mendelian Randomization and Colocalization Highlights the Bi-Directional Causal Association Between Hypothyroidism and Primary Biliary Cirrhosis
Authors: Wang Yanjun , Guo Ping , Zhang Yanan , Liu Lu , Yan Ran , Yuan Zhongshang , Song Yongfeng
Published: October 2021

Background: Hypothyroidism and primary biliary cirrhosis (PBC) are often co-existed in observational epidemiological studies. However, the causal relationship between them remains unclear.

Methods: Genetic correlation, Mendelian randomization (MR) and colocalization analysis were combined to assess the potential causal association between hypothyroidism and PBC by using summary statistics from large-scale genome-wide association studies. Various sensitivity analyses had been conducted to assess the robustness and the consistency of the findings.

Results: The linkage disequilibrium score regression demonstrated significant evidence of shared genetic architecture between hypothyroidism and PBC, with the genetic correlation estimated to be 0.117 (p = 0.006). The OR of hypothyroidism on PBC was 1.223 (95% CI, 1.072–1.396; p = 2.76 × 10−3) in MR analysis with inverse variance weighted (IVW) method. More importantly, the results from other 7MR methods with different model assumptions, were almost identical with that of IVW, suggesting the findings were robust and convincing. On the other hand, PBC was also causally associated with hypothyroidism (OR, 1.049; 95% CI, 1.010–1.089; p = 0.012), and, again, similar results can also be obtained from other MR methods. Various sensitivity analyses regarding the outlier detection and leave-one-out analysis were also performed. Besides, colocalization analysis suggested that there existed shared causal variants between hypothyroidism and PBC, further highlighting the robustness of the results.

Conclusion: Our results suggest evidence for the bi-directional causal association between hypothyroidism and PBC, which may provide insights into the etiology of hypothyroidism and PBC as well as inform prevention and intervention strategies directed toward both diseases.
Syndecan-1 Amplifies Ovalbumin-Induced Airway Remodeling by Strengthening TGFβ1/Smad3 Action
Authors: Zhang Dong , Qiao Xin-rui , Cui Wen-Jing , Zhang Jin-tao , Pan Yun , Liu Xiao-fei , Dong Liang
Published: October 2021

Syndecan-1 (SDC-1) is a transmembrane proteoglycan of heparin sulfate that can regulate various cell signal transduction pathways in the airway epithelial cells and fibroblasts. Airway epithelial cells and human bronchial fibroblasts are crucial in airway remodeling. However, the importance of SDC-1 in the remodeling of asthmatic airways has not been confirmed yet. The present study was the first to uncover SDC-1 overexpression in the airways of humans and mice with chronic asthma. This study also validated that an increase in SDC-1 expression was correlated with TGFβ1/Smad3-mediated airway remodeling in vivo and in vitro. A small interfering RNA targeting SDC-1 (SDC-1 siRNA) and homo-SDC-1 in pcDNA3.1 (pc-SDC-1) was designed to assess the effects of SDC-1 on TGFβ1/Smad3-mediated collagen I expression in Beas-2B (airway epithelial cells) and HLF-1 (fibroblasts) cells. Downregulation of the SDC-1 expression by SDC-1 siRNA remarkably attenuated TGFβ1-induced p-Smad3 levels and collagen I expression in Beas-2B and HLF-1 cells. In addition, SDC-1 overexpression with pc-SDC-1 enhanced TGFβ1-induced p-Smad3 level and collagen I expression in Beas-2B and HLF-1 cells. Furthermore, the levels of p-Smad3 and collagen I induced by TGFβ1 were slightly increased after the addition of the recombinant human SDC-1 protein to Beas-2B and HLF-1 cells. These findings in vitro were also confirmed in a mouse model. A short hairpin RNA targeting SDC-1 (SDC-1 shRNA) to interfere with SDC-1 expression considerably reduced the levels of p-Smad3 and remodeling protein (α-SMA, collagen I) in the airways induced by ovalbumin (OVA). Similarly, OVA-induced p-Smad3 and remodeling protein levels in airways increased after mice inhalation with the recombinant mouse SDC-1 protein. These results suggested that SDC-1 of airway epithelial cells and fibroblasts plays a key role in the development of airway remodeling in OVA-induced chronic asthma.
A Pan-Cancer Analysis of SMARCA4 Alterations in Human Cancers
Authors: Peng Ling , Li Jisheng , Wu Jie , Xu Bin , Wang Zhiqiang , Giamas Georgios , Stebbing Justin , Yu Zhentao
Published: October 2021

Background

SMARCA4, the essential ATPase subunit of SWI/SNF chromatin remodeling complex, regulates transcription through the control of chromatin structure and is increasingly thought to play significant roles in human cancers. This study aims to explore the potential role of SMARCA4 with a view to providing insights on pathologic mechanisms implicated here.

Methods

The potential roles of SMARCA4 in different tumors were explored based on The Cancer Genome Atlas (TCGA), Genotype-tissue expression (GTEx), Tumor Immune Estimation Resource (TIMER), and Gene Set Enrichment Analysis (GSEA) datasets. The expression difference, mutation and phosphorylation status, survival, pathological stage, DNA methylation, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repair (MMR), tumor microenvironment (TME), and immune cell infiltration related to SMARCA4 were analyzed.

Results

High expression levels of SMARCA4 were observed in most cancer types. SMARCA4 expression in tumor samples correlates with poor overall survival in several cancers. Lung adenocarcinoma cases with altered SMARCA4 showed a poorer prognosis. Enhanced phosphorylation levels of S613, S695, S699, and S1417 were observed in several tumors, including breast cancer. SMARCA4 correlated with tumor immunity and associated with different immune cells and genes in different cancer types. TMB, MSI, MMR, and DNA methylation correlated with SMARCA4 dysregulation in cancers. SMARCA4 expression was negatively associated with CD8+ T-cell infiltration in several tumors. Furthermore, the SWI/SNF superfamily-type complex and ATPase complex may be involved in the functional mechanisms of SMARCA4, albeit these data require further confirmation.

Conclusions

Our study offers a comprehensive understanding of the oncogenic roles of SMARCA4 across different tumors. SMARCA4 may correlate with tumor immunity.
Life in the Fast Lane: Modeling the Fate of Glass Sponge Larvae in the Gulf Stream
Authors: Wang Shuangqiang , Kenchington Ellen , Wang Zeliang , Davies Andrew J.
Published: October 2021

Effective conservation management of deep-sea sponges, including design of appropriate marine protected areas, requires an understanding of the connectivity between populations throughout a species’ distribution. We provide the first consideration of larval connectivity among deep-sea sponge populations along the southeastern coast of North America, illustrate the influence of the Gulf Stream on dispersal, and complement published distribution models by evaluating colonization potential. Connectivity among known populations of the hexactinellid sponge Vazella pourtalesii was simulated using a 3-D biophysical dispersal model throughout its distribution from Florida, United States to Nova Scotia, Canada. We found no exchange with an estimated pelagic larval duration of 2 weeks between populations north and south of Cape Hatteras, North Carolina at surface, mid-water and seabed release depths, irrespective of month of release or application of a horizontal diffusion constant specific to cross-Gulf Stream diffusivity. The population north of Cape Hatteras and south of Cape Cod was isolated. There was some evidence that Gulf Stream eddies formed near Cape Hatteras could travel to the northwest, connecting the populations in the two sub-regions, however that would require a much longer pelagic duration than what is currently known. More likely almost all larval settlement will be in the immediate area of the adults. At sub-regional scales, connectivity was found from the Strait of Florida through to the Blake Plateau, southeastern United States, with the latter area showing potential for recruitment from more than one source population. The influence of the Charleston Bump, a shallow feature rising from the Blake Plateau, was substantial. Particles seeded just north of the Bump were transported greater distances than those seeded to the south, some of which were caught in an associated gyre, promoting retention at the seabed. To the north on the Scotian Shelf, despite weaker currents and greater distances between known occurrences, unidirectional transport was detected from Emerald Basin to the Northeast Channel between Georges and Browns Banks. These major conclusions remained consistent through simulations run with different averaging periods for the currents (decades to daily) and using two ocean model products (BNAM and GLORYS12V1).
Automatic Segregation of Pelagic Habitats
Authors: Plonus Rene-Marcel , Vogl Stefanie , Floeter Jens
Published: October 2021

It remains difficult to segregate pelagic habitats since structuring processes are dynamic on a wide range of scales and clear boundaries in the open ocean are non-existent. However, to improve our knowledge about existing ecological niches and the processes shaping the enormous diversity of marine plankton, we need a better understanding of the driving forces behind plankton patchiness. Here we describe a new machine-learning method to detect and quantify pelagic habitats based on hydrographic measurements. An Autoencoder learns two-dimensional, meaningful representations of higher-dimensional micro-habitats, which are characterized by a variety of biotic and abiotic measurements from a high-speed ROTV. Subsequently, we apply a density-based clustering algorithm to group similar micro-habitats into associated pelagic macro-habitats in the German Bight of the North Sea. Three distinct macro-habitats, a “surface mixed layer,” a “bottom layer,” and an exceptionally “productive layer” are consistently identified, each with its distinct plankton community. We provide evidence that the model detects relevant features like the doming of the thermocline within an Offshore Wind Farm or the presence of a tidal mixing front.
A Novel Ventilator Design for COVID-19 and Resource-Limited Settings
Authors: Madekurozwa Michael , Bonneuil Willy V. , Frattolin Jennifer , Watson Daniel J. , Moore Axel C. , Stevens Molly M. , Moore James , Mathiszig-Lee Jakob , van Batenburg-Sherwood Joseph
Published: October 2021

There has existed a severe ventilator deficit in much of the world for many years, due in part to the high cost and complexity of traditional ICU ventilators. This was highlighted and exacerbated by the emergence of the COVID-19 pandemic, during which the increase in ventilator production rapidly overran the global supply chains for components. In response, we propose a new approach to ventilator design that meets the performance requirements for COVID-19 patients, while using components that minimise interference with the existing ventilator supply chains. The majority of current ventilator designs use proportional valves and flow sensors, which remain in short supply over a year into the pandemic. In the proposed design, the core components are on-off valves. Unlike proportional valves, on-off valves are widely available, but accurate control of ventilation using on-off valves is not straightforward. Our proposed solution combines four on-off valves, a two-litre reservoir, an oxygen sensor and two pressure sensors. Benchtop testing of a prototype was performed with a commercially available flow analyser and test lungs. We investigated the accuracy and precision of the prototype using both compressed gas supplies and a portable oxygen concentrator, and demonstrated the long-term durability over 15 days. The precision and accuracy of ventilation parameters were within the ranges specified in international guidelines in all tests. A numerical model of the system was developed and validated against experimental data. The model was used to determine usable ranges of valve flow coefficients to increase supply chain flexibility. This new design provides the performance necessary for the majority of patients that require ventilation. Applications include COVID-19 as well as pneumonia, influenza, and tuberculosis, which remain major causes of mortality in low and middle income countries. The robustness, energy efficiency, ease of maintenance, price and availability of on-off valves are all advantageous over proportional valves. As a result, the proposed ventilator design will cost significantly less to manufacture and maintain than current market designs and has the potential to increase global ventilator availability.
The Unifying Hypothesis of Alzheimer’s Disease: Heparan Sulfate Proteoglycans/Glycosaminoglycans Are Key as First Hypothesized Over 30 Years Ago
Authors: Snow Alan David , Cummings Joel A. , Lake Thomas
Published: October 2021

The updated “Unifying Hypothesis of Alzheimer’s disease” (AD) is described that links all the observed neuropathology in AD brain (i.e., plaques, tangles, and cerebrovascular amyloid deposits), as well as inflammation, genetic factors (involving ApoE), “AD-in-a-Dish” studies, beta-amyloid protein (Aβ) as a microbial peptide; and theories that bacteria, gut microflora, gingivitis and viruses all play a role in the cause of AD. The common link is the early accumulation of heparan sulfate proteoglycans (HSPGs) and heparan sulfate glycosaminoglycans (GAGs). HS GAG accumulation and/or decreased HS GAG degradation is postulated to be the key initiating event. HS GAGs and highly sulfated macromolecules induce Aβ 1–40 (but not 1–42) to form spherical congophilic maltese-cross star-like amyloid core deposits identical to those in the AD brain. Heparin/HS also induces tau protein to form paired helical filaments (PHFs). Increased sulfation and/or decreased degradation of HSPGs and HS GAGs that occur due to brain aging leads to the formation of plaques and tangles in AD brain. Knockout of HS genes markedly reduce the accumulation of Aβ fibrils in the brain demonstrating that HS GAGs are key. Bacteria and viruses all use cell surface HS GAGs for entry into cells, including SARS-CoV-2. Bacteria and viruses cause HS GAGs to rapidly increase to cause near-immediate aggregation of Aβ fibrils. “AD-in-a-dish” studies use “Matrigel” as the underlying scaffold that spontaneously causes plaque, and then tangle formation in a dish. Matrigel mostly contains large amounts of perlecan, the same specific HSPG implicated in AD and amyloid disorders. Mucopolysaccharidoses caused by lack of specific HS GAG enzymes lead to massive accumulation of HS in lysosomal compartments in neurons and contribute to cognitive impairment in children. Neurons full of HS demonstrate marked accumulation and fibrillization of Aβ, tau, α-synuclein, and prion protein (PrP) in mucopolysaccharidosis animal models demonstrating that HS GAG accumulation is a precursor to Aβ accumulation in neurons. Brain aging leads to changes in HSPGs, including newly identified splice variants leading to increased HS GAG sulfation in the AD brain. All of these events lead to the new “Unifying Hypothesis of Alzheimer’s disease” that further implicates HSPGs /HS GAGs as key (as first hypothesized by Snow and Wight in 1989).