bluuebunny/crossref_metadata_2025 huggingface dataset:
Kinematics Parameter Calibration of Serial Industrial Robots Based on Partial Pose Measurement
Authors: Xiang Tiewu , Jiang Xinyi , Qiao Guifang , Gao Chunhui , Zuo Hongfu
Published: November 2023

The kinematics parameter error is the main error factor that affects the absolute accuracy of industrial robots. The absolute accuracy of industrial robots can be effectively improved through kinematics calibration. The error model-based method is one of the main methods for calibrating the kinematics parameter error. This paper presents a kinematics parameter calibration method for serial industrial robots based on partial pose measurement. Firstly, the kinematics and the pose error models have been established based on the modified Denavit–Hartenberg (MDH) model. By introducing the concept of error sensitivity, the average significance index is proposed to quantitatively analyze the effects of the kinematics parameter error on the pose error of a robot. The results show that there is no need to measure the full pose error of the robot. Secondly, a partial pose measurement device and method have been presented. The proposed device can measure the position error and the attitude error on the x-axis or y-axis. Finally, the full pose error model, the NP-type partial pose error model, and the OP-type partial pose error model have been applied for calibrating the kinematics parameter errors. The experimental results show that the effectiveness of the OP-type partial pose error model is consistent with the full pose error model.
In-Plane Wave Propagation Analysis of Human Breast Lesions Using a Higher-Order Nonlocal Model and Deep Learning
Authors: Farajpour Ali , Ingman Wendy V.
Published: November 2023

The wave propagation characteristics of biological tissues are of high importance in improving healthcare technologies and can be used as an early clinical indicator of many diseases. However, the current mathematical models that describe the mechanical properties of biological tissues do not account for the difference in softening and hardening observed at different scales and this limits their utility in biomedical imaging. In this paper, a higher-order nonlocal model is developed to study in-plane wave propagation in healthy, benign, and cancerous breast tissues. To verify the mathematical approach, finite element simulations are conducted. Furthermore, a sequential deep neural network model of feedforward type with multiple hidden layers is developed to understand the intrinsic in-plane wave characteristics of breast tissues. The deep learning algorithm shows potential in accurately extracting the frequencies and phase velocities of breast lesions under in-plane waves even when there is a limited number of clinical samples. Using the higher-order nonlocal model, significant differences between healthy fibroglandular tissue and early breast cancer in the form of ductal carcinoma in situ have been found. The combination of nonlocal and strain gradient parameters allows for the concurrent incorporation of stiffness hardening and softening, solving the rigid-tumour–soft-cell paradox of cancer biomechanics.
Bifurcation Behavior and Hybrid Controller Design of a 2D Lotka–Volterra Commensal Symbiosis System Accompanying Delay
Authors: Cui Qingyi , Xu Changjin , Ou Wei , Pang Yicheng , Liu Zixin , Li Peiluan , Yao Lingyun
Published: November 2023

All the time, differential dynamical models with delay has witness a tremendous application value in characterizing the internal law among diverse biological populations in biology. In the current article, on the basis of the previous publications, we formulate a new Lotka–Volterra commensal symbiosis system accompanying delay. Utilizing fixed point theorem, inequality tactics and an appropriate function, we gain the sufficient criteria on existence and uniqueness, non-negativeness and boundedness of the solution to the formulated delayed Lotka–Volterra commensal symbiosis system. Making use of stability and bifurcation theory of delayed differential equation, we focus on the emergence of bifurcation behavior and stability nature of the formulated delayed Lotka–Volterra commensal symbiosis system. A new delay-independent stability and bifurcation conditions on the model are presented. By constructing a positive definite function, we explore the global stability. By constructing two diverse hybrid delayed feedback controllers, we can adjusted the domain of stability and time of appearance of Hopf bifurcation of the delayed Lotka–Volterra commensal symbiosis system. The effect of time delay on the domain of stability and time of appearance of Hopf bifurcation of the model is given. Matlab experiment diagrams are provided to sustain the acquired key outcomes.
Approximate Closed-Form Solutions for a Class of 3D Dynamical Systems Involving a Hamilton–Poisson Part
Authors: Ene Remus-Daniel , Pop Nicolina
Published: November 2023

The goal of this paper is to build some approximate closed-form solutions for a class of dynamical systems involving a Hamilton–Poisson part. The chaotic behaviors are neglected. These solutions are obtained by means of a new version of the optimal parametric iteration method (OPIM), namely, the modified optimal parametric iteration method (mOPIM). The effect of the physical parameters is investigated. The Hamilton–Poisson part of the dynamical systems is reduced to a second-order nonlinear differential equation, which is analytically solved by the mOPIM procedure. A comparison between the approximate analytical solution obtained with mOPIM, the analytical solution obtained with the iterative method, and the corresponding numerical solution is presented. The mOPIM technique has more advantages, such as the convergence control (in the sense that the residual functions are smaller than 1), the efficiency, the writing of the solutions in an effective form, and the nonexistence of small parameters. The accuracy of the analytical and corresponding numerical results is illustrated by graphical and tabular representations. The same procedure could be successfully applied to more dynamical systems.
Deep-Sea Sponges and Corals off the Western Coast of Florida—Intracellular Mechanisms of Action of Bioactive Compounds and Technological Advances Supporting the Drug Discovery Pipeline
Authors: Iskandar Mina , Ruiz-Houston Kira M. , Bracco Steven D. , Sharkasi Sami R. , Calabi Villarroel Cecilia L. , Desai Meghna N. , Gerges Alexandra G. , Ortiz Lopez Natalia A. , Xiao Barbero Miguel , German Amelia A. , Moluguri Vinoothna S. , Walker Selina M. , Silva Higashi Juliana , Palma Justin M. , Medina Daena Z. , Patel Miit , Patel Prachi , Valentin Michaela , Diaz Angelica C. , Karthaka Jonathan P. , Santiago Atzin D. , Skiles Riley B. , Romero Umana Luis A. , Ungrey Maxwell D. , Wojtkowiak Anya , Howard Domenica V. , Nurge Remy , Woods Katharine G. , Nanjundan Meera
Published: November 2023

The majority of natural products utilized to treat a diverse array of human conditions and diseases are derived from terrestrial sources. In recent years, marine ecosystems have proven to be a valuable resource of diverse natural products that are generated to defend and support their growth. Such marine sources offer a large opportunity for the identification of novel compounds that may guide the future development of new drugs and therapies. Using the National Oceanic and Atmospheric Administration (NOAA) portal, we explore deep-sea coral and sponge species inhabiting a segment of the U.S. Exclusive Economic Zone, specifically off the western coast of Florida. This area spans ~100,000 km2, containing coral and sponge species at sea depths up to 3000 m. Utilizing PubMed, we uncovered current knowledge on and gaps across a subset of these sessile organisms with regards to their natural products and mechanisms of altering cytoskeleton, protein trafficking, and signaling pathways. Since the exploitation of such marine organisms could disrupt the marine ecosystem leading to supply issues that would limit the quantities of bioactive compounds, we surveyed methods and technological advances that are necessary for sustaining the drug discovery pipeline including in vitro aquaculture systems and preserving our natural ecological community in the future. Collectively, our efforts establish the foundation for supporting future research on the identification of marine-based natural products and their mechanism of action to develop novel drugs and therapies for improving treatment regimens of human conditions and diseases.
Marine-Fungi-Derived Gliotoxin Promotes Autophagy to Suppress Mycobacteria tuberculosis Infection in Macrophage
Authors: Fu Jun , Luo Xiaowei , Lin Miaoping , Xiao Zimin , Huang Lishan , Wang Jiaxi , Zhu Yongyan , Liu Yonghong , Tao Huaming
Published: November 2023

The Mycobacterium tuberculosis (MTB) infection causes tuberculosis (TB) and has been a long-standing public-health threat. It is urgent that we discover novel antitubercular agents to manage the increased incidence of multidrug-resistant (MDR) or extensively drug-resistant (XDR) strains of MTB and tackle the adverse effects of the first- and second-line antitubercular drugs. We previously found that gliotoxin (1), 12, 13-dihydroxy-fumitremorgin C (2), and helvolic acid (3) from the cultures of a deep-sea-derived fungus, Aspergillus sp. SCSIO Ind09F01, showed direct anti-TB effects. As macrophages represent the first line of the host defense system against a mycobacteria infection, here we showed that the gliotoxin exerted potent anti-tuberculosis effects in human THP-1-derived macrophages and mouse-macrophage-leukemia cell line RAW 264.7, using CFU assay and laser confocal scanning microscope analysis. Mechanistically, gliotoxin apparently increased the ratio of LC3-II/LC3-I and Atg5 expression, but did not influence macrophage polarization, IL-1β, TNF-a, IL-10 production upon MTB infection, or ROS generation. Further study revealed that 3-MA could suppress gliotoxin-promoted autophagy and restore gliotoxin-inhibited MTB infection, indicating that gliotoxin-inhibited MTB infection can be treated through autophagy in macrophages. Therefore, we propose that marine fungi-derived gliotoxin holds the promise for the development of novel drugs for TB therapy.
Assessing Fatigue Life Cycles of Material X10CrMoVNb9-1 through a Combination of Experimental and Finite Element Analysis
Authors: Rahim Mohammad Ridzwan Bin Abd , Schmauder Siegfried , Manurung Yupiter H. P. , Binkele Peter , Dusza Ján , Csanádi Tamás , Ahmad Meor Iqram Meor , Mat Muhd Faiz , Dogahe Kiarash Jamali
Published: November 2023

This paper uses a two-scale material modeling approach to investigate fatigue crack initiation and propagation of the material X10CrMoVNb9-1 (P91) under cyclic loading at room temperature. The Voronoi tessellation method was implemented to generate an artificial microstructure model at the microstructure level, and then, the finite element (FE) method was applied to identify different stress distributions. The stress distributions for multiple artificial microstructures was analyzed by using the physically based Tanaka–Mura model to estimate the number of cycles for crack initiation. Considering the prediction of macro-scale and long-term crack formation, the Paris law was utilized in this research. Experimental work on fatigue life with this material was performed, and good agreement was found with the results obtained in FE modeling. The number of cycles for fatigue crack propagation attains up to a maximum of 40% of the final fatigue lifetime with a typical value of 15% in many cases. This physically based two-scale technique significantly advances fatigue research, particularly in power plants, and paves the way for rapid and low-cost virtual material analysis and fatigue resistance analysis in the context of environmental fatigue applications.
Effect of Nanostructuring on Operational Properties of 316LVM Steel
Authors: Rybalchenko Olga , Anisimova Natalia , Martynenko Natalia , Rybalchenko Georgy , Tokar Alexey , Lukyanova Elena , Prosvirnin Dmitry , Gorshenkov Mikhail , Kiselevskiy Mikhail , Dobatkin Sergey
Published: November 2023

In this study, high-pressure torsion (HPT) was used to process austenitic 316LVM stainless steel at 20 °C and 400 °C. The effects of HPT on the microstructure, mechanical, and functional properties of the steel were investigated. By applying both HPT modes on the 316LVM steel, a nanocrystalline state with an average size of the structural elements of ~46–50 nm was achieved. The density of the dislocations and twins present in the austenite phase varied depending on the specific HPT conditions. Despite achieving a similar structural state after HPT, the deformation temperatures used has different effects on the mechanical and functional properties of the steel. After HPT at 20 °C, the yield strength of the 316L steel increased by more than nine times up to 1890 MPa, and the fatigue limit by more than two times up to 550 MPa, when compared to its coarse-grained counter-parts. After HPT at 20 °C, the 316LVM steel exhibited better ductility, higher low-cycle fatigue resistance, greater resistance to corrosion, and improved in vitro biocompatibility compared to processing at 400 °C. The reasons for the deterioration of the properties after HPT at 400 °C are discussed in the article.
Variations in the Crystal Lattice of Tb-Dy-Fe Magnetostrictive Materials: The Lattice Constant Disturbance
Authors: Gong Jiaxin , Li Jiheng , Bao Xiaoqian , Gao Xuexu
Published: November 2023

In Tb-Dy-Fe alloy systems, Tb0.29Dy0.71Fe1.95 alloy shows giant magnetostrictive properties under low magnetic fields, thus having great potential for transducers, microsensors, and other applications. The C15 cubic crystal structure of Tb-Dy-Fe has long been thought to be the source of giant magnetostriction. It is surprising that such a highly symmetrical crystal structure exhibits such a large magnetostrictive strain. In this work, the lattice parameters of Tb0.29Dy0.71Fe1.95 magnetostrictive materials were studied by processing atomic-resolution images. The selected area diffraction patterns show a face-centered cubic structure, but the fast Fourier transform diagram shows that the cubic structure has obvious distortion. The lattice parameters obtained by geometric phase analysis (GPA) and Gaussian model-based fitting and calculation show that the lattice constants a, b, and c are not strictly equal, and small disturbance of the lattice constants occurs based on the cubic structure. The actual crystal structure of the Tb-Dy-Fe material is a slightly disturbed cubic structure. This variation in the crystal lattice is mainly caused by the inhomogeneous composition and may be related to the giant magnetostrictive properties of Tb-Dy-Fe alloy.
The Impact and Determinants of Mountainous Topographical Factors on Soil Microbial Community Characteristics
Authors: Yu Jiantao , Li Suyan , Sun Xiangyang , Zhou Wenzhi , He Libing , Zhao Guanyu , Chen Zhe , Bai Xueting , Zhang Jinshuo
Published: November 2023

Soil bacterial and fungal community communities play significant ecological functions in mountain ecosystems. However, it is not clear how topographic factors and soil physicochemical properties influence changes in microbial community structure and diversity. This study aims to investigate how altitude and slope orientation affect soil physicochemical properties, soil microbial communities, and their contributing factors. The assessment was conducted using Illumina MiSeq sequencing in various altitude gradients and on slopes with different aspects (shady slopes and sunny slopes) in the subalpine meadow of Dongling Mountain, Beijing. Topographical factors had a significant effect on soil physicochemical properties: the primary factors determining the structure of microbial communities are total potassium (TK), ammonium nitrogen (NH4+-N), and soil organic carbon (SOC). There was no significant change in the diversity of the bacterial community, whereas the diversity of the fungal community displayed a single-peaked trend. The effect of slope orientation on microbial communities was not as significant as the effect of elevation on them. The number of bacterial communities with significant differences showed a unimodal trend, while the number of fungal communities showed a decreasing trend. The co-occurrence network of fungal communities exhibits greater intricacy than that of bacterial communities, and bacterial communities are more complex in soils with sunny slopes compared to soils with shady slopes, and the opposite is true for fungal communities. The identification of the main factors that control soil microbial diversity and composition in this study, provided the groundwork for investigating the soil microbial response and adaptation to environmental changes in subalpine meadows.
Soft Measurement of Rare Earth Multi-Element Component Content Based on Multi-LightVGG Modeling
Authors: Li Zhen , Xiao Jun , Zhang Qihan , Liu Kunming , Li Jinhui
Published: November 2023

The current hardware equipment used to detect the content of each element component in the rare earth extraction process has a complex structure and high maintenance cost. A modeling method for the soft measurement of rare earth multi-element component content is proposed to address this issue. This method uses the Multi-LightVGG multi-tasking learning model and the Multi Gradient Descent Algorithm based on Optimized Upper Bound (MGDA-OUB) to optimize the model for each prediction task and find the Pareto optimal solution. After conducting several experiments, the Multi-LightVGG model loaded with MGDA-OUB has lower MRE, RMSE for Pr, Nd prediction, and MAX(|error|) for Nd prediction than the Multi-LightVGG model without MGDA-OUB by 0.3778%, 0.5208%, 0.0015, 0.0015, and 0.1985%, respectively; and the MRE and RMSE of the Multi-LightVGG model for Pr and Nd prediction under the same optimization conditions are lower than those of Multi-ResNet18 by 0.3297%, 0.5423%, 0.0019, and 0.002, respectively, thus indicating that MGDA-OUB can effectively solve multiple task-specific Pareto solutions to avoid possible conflicts between specific tasks, while the Multi-LightVGG model, compared to the Multi-Resnet18 model, has a backbone network that can effectively capture the abstract representations in the images of the rare earth-extraction mixed solution, which in turn improves the prediction accuracy of the content of each elemental component.
Preliminary Study on the Surface Modification of Lignite and Bioflotation by White-Rot Fungi Hypocrea lixii AH
Authors: He Huan , Cao Mingjun , Zhan Di , Xia Wencheng , Chen Songjiang , Tao Xiuxiang , Huang Zaixing
Published: November 2023

The efficient utilization of lignite is a crucial area of research for the sustainable management of existing coal resources. One potential technique for cost-effective and environmentally friendly coal processing is the application of microbes or their derivatives to modify the surface of lignite for bioflotation. However, the precise process of surface modification between microbes and coal remains largely unknown. In this study, we focused on the use of a white-rot fungus called Hypocrea lixii AH and its various components, including spores, hyphae, extracellular polymer substances (EPSs), and culture solution, as biosurfactants for lignite modification. By employing techniques such as zeta potential analysis, induction time measurement, contact angle measurement, and Fourier infrared spectroscopy, we investigated the changes in the surface properties of raw and modified lignite. Furthermore, we conducted a preliminary bioflotation test using biosurfactants as collectors in order to explore the potential application of fungal modification in this context. Our results revealed that all biosurfactants were effective in improving the surface properties of lignite, with the EPS demonstrating the most prominent effect, followed by the culture solution, hyphae, and spores. The zeta potential and induction time of the modified lignite decreased, indicating enhanced hydrophilicity, while the contact angle exhibited a slight increase, suggesting a minor increase in hydrophobicity. Analysis of the Fourier infrared spectra indicated that EPS treatment resulted in the highest abundance of functional groups, including carboxyl, hydroxyl, and amidogen groups. Although fungal cells were found to improve the hydrophobicity of coal, they did not exhibit a significant effect on the flotation of lignite. Nonetheless, our findings suggest that fungal cells and their derivatives have the potential to remove or transform minerals present in lignite, particularly those containing sulfur. While they may not serve as effective bio-collectors in microflotation, their capability in mineral alteration makes them valuable candidates for lignite processing with a focus on mineral reduction.
Lewis Acid-Base Adducts of α-Amino Acid-Derived Silaheterocycles and N-Methylimidazole
Authors: Seidel Anne , Gericke Robert , Kutzner Beate , Wagler Jörg
Published: November 2023

In chloroform solution, the reaction of bis(tert-butylamino)dimethylsilane ((tBuNH)2SiMe2) and an α-amino acid (α-amino isobutyric acid, H2Aib; D-phenylglycine, H2Phg; L-valine, H2Val) in the presence of N-methylimidazole (NMI) gave rise to the formation of the pentacoordinate silicon complexes (Aib)SiMe2-NMI, (Phg)SiMe2-NMI and (Val)SiMe2-NMI, respectively. Therein, the amino acid building block was a di-anionic bidentate chelator at the silicon atom. In solution, the complexes were involved in rapid coordination–dissociation equilibria between the pentacoordinate Si complex (e.g., (Aib)SiMe2-NMI) and its constituents NMI and a five-membered silaheterocycle (e.g., (Aib)SiMe2), as shown by 29Si NMR spectroscopy. The energetics of the Lewis acid-base adduct formation and the competing solvation of the NMI molecule by chloroform were assessed with the aid of computational methods. In CDCl3 solution, deuteration of the silaheterocycle NH group proceeded rapidly, with more than 50% conversion within two days. Upon cooling to −44 °C, the chloroform solvates of the adducts (Aib)SiMe2-NMI and (Phg)SiMe2-NMI crystallized from their parent solutions and allowed for their single-crystal X-ray diffraction analyses. In both cases, the Si atom was situated in a distorted trigonal bipyramidal coordination sphere with equatorial Si–C bonds and an equatorial Si–N bond (the one of the silaheterocycle). The axial positions were occupied by a carboxylate O atom of the silaheterocycle and the NMI ligand’s donor-N-atom.
Actinidia arguta (Sieb. et Zucc.) Planch. ex Miq.: A Review of Phytochemistry and Pharmacology
Authors: Zhang Haifeng , Teng Kun , Zang Hao
Published: November 2023

Actinidia arguta (Siebold & Zucc.) Planch ex Miq. (A. arguta) is a highly valued vine plant belonging to the Actinidia lindl genus. It is extensively utilized for its edible and medicinal properties. The various parts of A. arguta serve diverse purposes. The fruit is rich in vitamins, amino acids, and vitamin C, making it a nutritious and flavorful raw material for producing jam, canned food, and wine. The flowers yield volatile oils suitable for essential oil extraction. The leaves contain phenolic compounds and can be used for tea production. Additionally, the roots, stems, and leaves of A. arguta possess significant medicinal value, as they contain a wide array of active ingredients that exert multiple pharmacological and therapeutic effects. These effects include quenching thirst, relieving heat, stopping bleeding, promoting blood circulation, reducing swelling, dispelling wind, and alleviating dampness. Comprehensive information on A. arguta was collected from scientific databases covering the period from 1970 to 2023. The databases used for this review included Web of Science, PubMed, ProQuest, and CNKI. The objective of this review was to provide a detailed explanation of A. arguta from multiple perspectives, such as phytochemistry and pharmacological effects. By doing so, it aimed to establish a solid foundation and propose new research ideas for further exploration of the plant’s potential applications and industrial development. To date, a total of 539 compounds have been isolated and identified from A. arguta. These compounds include terpenoids, flavonoids, phenolics, phenylpropanoids, lignin, organic acids, volatile components, alkanes, coumarins, anthraquinones, alkaloids, polysaccharides, and inorganic elements. Flavonoids, phenolics, alkaloids, and polysaccharides are the key bioactive constituents of A. arguta. Moreover, phenolics and flavonoids in A. arguta exhibit remarkable antioxidant, anti-inflammatory, and anti-tumor properties. Additionally, they show promising potential in improving glucose metabolism, combating aging, reducing fatigue, and regulating the immune system. While some fundamental studies on A. arguta have been conducted, further research is necessary to enhance our understanding of its mechanism of action, quality evaluation, and compatibility mechanisms. A more comprehensive investigation is highly warranted to explore the mechanism of action and expand the range of drug resources associated with A. arguta. This will contribute to the current hot topics of anti-aging and anti-tumor drug research and development, thereby promoting its further development and utilization.
Preparation and Self-Cleaning Performance of High-Strength Double-Layer PVDF-PVC-Nano-Graphite/PVDF-PVC Super-Hydrophobic Composite Membrane
Authors: Zhang Dashuai , Sun Shishu , Zhang Yan , Zhang Xiaopeng , Li Chen , Sun Tianyi , Zhu Linhua , Shi Zaifeng
Published: November 2023

The double-layer PVDF-PVC (D-PP/PP) super-hydrophobic composite membrane was prepared by the coating immersion phase separation method to enhance the mechanical properties of the composite membrane. The D-PP/PP super-hydrophobic membrane was prepared using the casting solution concentration of 12 wt% PVDF-PVC composite membrane as basement and 4% casting of PVDF-PVC coating. The contact angle of the D-PP/PP membrane was 150.4 ± 0.3°, and the scanning electron microscope showed that the surface of the D-PP/PP membrane was covered by a cross-linked micro–nano microsphere. The mechanical properties showed that the maximum tensile force of the D-PP/PP composite membrane was 2.34 N, which was 19.4% higher than that of PVDF-PVC (1.96 N). Nano-graphite was added to the coating layer in the experiment. The prepared double-layer PVDF-PVC-nano-graphite/PVDF-PVC (D-PPG/PP) composite membrane reached 153.7 ± 0.5°, the contact angle increasing by 3.3°. The SEM comparison showed that the D-PPG/PP composite membrane had a more obvious micro–nano level microsphere layer. The mechanical properties are also superior. By preparing the D-PP/PP membrane, the mechanical properties of the membrane were improved, and the super-hydrophobic property of the coating was also obtained. At the same time, it was found that adding nano-graphite to the coating layer can better improve the hydrophobic, mechanical, and self-cleaning properties of the D-PP/PP composite membrane.
Phase Transition Behaviors of Poly(N-isopropylacrylamide) Nanogels with Different Compositions Induced by (−)-Epigallocatechin-3-gallate and Ethyl Gallate
Authors: Deng Ke , Wang Yafei , Wang Lei , Fan Xianli , Wu Zhenyu , Wen Xue , Xie Wen , Wang Hong , Zhou Zheng , Chen Pengfei , Chen Xianggui
Published: November 2023

Phase transition behaviors of poly(N-isopropylacrylamide) nanogels with different compositions induced by (−)-epigallocatechin-3-gallate (EGCG) and ethyl gallate (EG) has been investigated systematically. Monodisperse poly(N-isopropylacrylamide-co-N-hydroxymethyl acrylamide) (P(NIPAM-co-NMAM)) and poly(N-isopropylacrylamide-co-2-hydroxyethyl methacrylate) (P(NIPAM-co-HEMA)) nanogels with different feeding monomer ratios were prepared by emulsion polymerization. P(NIPAM-co-NMAM) nanogels exhibit rapid isothermal phase transition behavior in EGCG solutions with low concentration (10−3 mol/L) in less than 10 minutes. The thermosensitive phase transition behaviors of nanogels are affected not only by the copolymerized monomers but also by the concentrations of EGCG and EG in aqueous solutions. Nanogels remain in a shrunken state and do not exhibit thermosensitive phase transition behaviors in EGCG solutions (≥5 mmol/L), whereas they display thermo-responsive phase transition behaviors in EG solutions. The volume phase transition temperature (VPTT) shifts to lower temperatures with increasing EG concentration. The diameters of P(NIPAM-co-NMAM) nanogels decrease with increasing EG concentration at temperatures between 29 and 33 °C. In contrast, the diameters of P(NIPAM-co-HEMA) nanogels increase with increasing EGCG concentration at temperatures between 37 and 45 °C. The results demonstrate the potential of nanogels for simple detection of EG and EGCG concentrations in aqueous solutions over a wide temperature range, and EGCG can serve as a signal for the burst-release of drugs from the P(NIPAM-co-NMAM)-based carriers at physiological temperature.
Physical Properties and Release Profiles of Chitosan Mixture Films Containing Salicin, Glycerin and Hyaluronic Acid
Authors: Lewandowska Katarzyna , Sionkowska Alina , Kurzawa Marzanna
Published: November 2023

Chitosan (CS) has gained considerable attention due to its distinctive properties and its broad spectrum of potential applications, spanning cosmetics, pharmaceuticals, and biomedical uses. In this study, we characterized thin films comprising chitosan mixtures containing salicin (SAL) and glycerin (GLY), both with and without hyaluronic acid (HA) as active ingredients. Characterization was achieved through release studies of SAL, infrared spectroscopy, microscopy techniques (AFM and SEM), and thermogravimetric analysis (TGA). CS/GLY/SAL and CS/GLY/SAL/HA mixture films were fabricated using the solvent evaporation technique. We probed interactions between the components in the chitosan mixtures via infrared analysis. The concentration of released salicin was monitored at various time intervals in a phosphate buffer (PBS) at pH 5.5 using HPLC. The linear regression analysis for the calibration graph showed a good linear relationship (R2 = 0.9996) in the working concentration range of 5–205 mg/dm3. Notably, the release of SAL reached its peak after 20 min. Furthermore, the introduction of HA caused changes in the films’ morphology, but their roughness remained largely unchanged. The results obtained were compared, indicating that the release of SAL in the CS mixture films is sufficient for diverse applications, including wound-healing materials and cosmetic beauty masks.
Advanced Nanostructured Coatings Based on Doped TiO2 for Various Applications
Authors: Gartner Mariuca , Szekeres Anna , Stroescu Hermine , Mitrea Daiana , Covei Maria
Published: November 2023

For many years, TiO2-based materials and improving their properties in order to expand their application areas have been the focus of numerous research groups. Various innovative approaches have been proposed to improve the photocatalytic and gas-sensing properties of TiO2 nanostructures. In this review, we aim to synthesize the available information in the literature, paying special attention to the sol–gel technology, which is one of the most frequently used methods for TiO2 synthesis. The influence of dopants on the structural, morphological, optical, and electrical properties of TiO2 and the way to modify them in a controlled manner are briefly discussed. The role of shallow and/or deep energy levels within the TiO2 bandgap in the electron transport behavior of doped TiO2 is emphasized. Selected research on photocatalytic applications in water disinfection, wastewater treatment, and self-sterilizing coatings that contribute to improving the quality of human life and environmental preservation is highlighted. A survey of biosensors that are closely related to medical applications such as cancer detection, implantology, and osteogenesis is also provided. Finally, the pressing problems that need to be solved in view of the future development of TiO2-based nanostructures are listed.
Network Analysis and Experimental Verification of the Mechanisms of Hydroxysafflor Yellow A in Ischemic Stroke Following Atherosclerosis
Authors: Han Xi , Zhou Huifen , Yin Junjun , Zhu Jiaqi , Yang Jiehong , Wan Haitong
Published: November 2023

Hydroxysafflor yellow A (HSYA) is derived from Carthamus tinctorius L. (Honghua in Chinese) and is used to treat cardiovascular and cerebrovascular disease. However, the mechanism by which HSYA treats ischemic stroke following atherosclerosis (ISFA) remains unclear. The targets and pathways of HSYA against ISFA were obtained using network analysis. A total of 3335 potential IFSA-related targets were predicted using the GenCards and Drugbank databases, and a total of 88 potential HSYA-related targets were predicted using the Swiss Target Prediction database. A total of 62 HSYA-related targets against IFSA were obtained. The network was composed of HSYA, 62 targets, and 20 pathways. The top 20 targets were constructed via the protein–protein interaction (PPI) network. Gene Ontology analysis revealed that the targets were involved in signal transduction, protein phosphorylation, the cytoplasm, the plasma membrane, the cytosol, zinc ion binding, ATP binding, protein kinase binding/activity, and enzyme binding. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the pathways were associated with cancer, inflammatory mediator regulation of the transient receptor potential channels, and microRNA in cancer. Additionally, molecular docking indicated that HSYA mainly interacts with five targets, namely interleukin 1 beta (IL-1β), signal transducer and activator of transcription 3 (STAT3), E1A-binding protein p300 (EP300), protein kinase C alpha (PRKCA), and inhibitor of nuclear factor kappa B kinase subunit beta (IKBKB). In animal experiments, HSYA administration ameliorated the infarct size, neurological deficit score, histopathological changes, carotid intima-media thickness (IMT), and blood lipid level (total cholesterol and triglycerides). Immunochemistry and quantitative PCR showed that HSYA intervention downregulated the expression of STAT3, EP300, PRKCA, and IKBKB, and the enzyme-linked immunoassay showed reduced IL-1β levels. The findings of this study provide a reference for the development of anti-ISFA drugs.
Electrical Conductivity of Ionic Liquids 1-Hexyl-3-Methylimidazolium Chloride (HMIM) and 1-Methyl-3-Octylimidazolium Chloride (OMIM) in Mixtures with Selected Alkoxy Alcohols over a Wide Temperature Range
Authors: Kinart Zdzisław
Published: November 2023

Ionic liquids have been the subject of intense research because of their unique electrochemical properties and potential applications in various fields. In this article, we analyze the electrical conductivity of two selected ionic liquids, 1-hexyl-3-methylimidazolium chloride (HMIM) and 1-eethyl-3-octylimidazolium chloride (OMIM), in various alkoxy alcohols such as 2-methoxyethanol, 2-ethoxyethanol, 2-propoxyethanol and 2-butoxyethanol. Our research focuses on attempting to analyze the impact of the molecular structure of both the ionic liquids and alkoxy alcohols on their electrical conductivity properties. The results of our study can be highly beneficial in the design of advanced electrochemical materials and their various applications.
Coupled Precipitation of Dual-Nanoprecipitates to Optimize Microstructural and Mechanical Properties of Cast Al–Cu–Mg–Mn Alloys via Modulating the Mn Contents
Authors: Zhang Han , Hao Qitang , Li Xinlei , Yu Wentao , Xue Yanqing
Published: November 2023

The effect of Mn content on the microstructure evolution and mechanical properties of Al–Cu–Mg–x Mn alloys at ambient temperature was investigated. The findings show that in the Mn-containing alloys at the as-cast state, the blocky primary T(Al20Cu2Mn3) phase coexisting with the Al2Cu phase appeared. With the increase in Mn content, the majority of the Al2Cu phase dissolved, nd a minor amount of the T phase remained at the grain boundary after solution treatment. The rod-like TMn (Al20Cu2Mn3) nanoprecipitate was simultaneously distributed at grain boundaries and the interiors, while a high density of needle-like θ″ (Al3Cu) nanoprecipitate was also observed in the T6 state. Further increases in Mn content promoted the dispersion of the TMn phase and inhibited the growth and transformation of the θ″ phase. Tensile test results show that 0.7 wt.% Mn alloy had excellent mechanical properties at ambient temperature with ultimate tensile strength, yield strength, and fracture elongation of 498.7 MPa, 346.2 MPa, and 19.2%, respectively. The subsequent calculation of strengthening mechanisms elucidates that precipitation strengthening is the main reason for the increase in yield strength of Mn-containing alloys.
Validation of the Childhood Family Mealtime Questionnaire in Mexican Adolescents with Obesity and Their Caregivers
Authors: Silva-Uribe Melissa , Máynez-López Fernanda , Denova-Gutiérrez Edgar , Muñoz-Guerrero Brenda , Omaña-Guzmán Isabel , Messiah Sarah E. , Ruíz-Arroyo Arturo , Lozano-González Emilia , Villanueva-Ortega Eréndira , Muñoz-Aguirre Paloma , Garibay-Nieto Nayely
Published: November 2023

Background: Childhood obesity is a significant public health concern in Mexico, with far-reaching implications for the nation’s healthcare system and economy. In light of this challenge, our study sought to validate the Childhood Family Mealtime Questionnaire (CFMQ) in Mexican adolescents living with obesity and their primary caregivers. Methods: A sample of 56 adolescents ages 13 to 17 years and their primary caregivers from one pediatric obesity clinic participated in the study. We conducted a comprehensive assessment of the CFMQ’s consistency, reliability, and construct validity among all participants. Internal consistency was determined using Cronbach’s α, and the questionnaire’s reliability was assessed through test–retest and intraclass correlation coefficients. Construct validity was assessed through an exploratory factor analysis. Results: Our findings confirmed strong internal consistency and reliability for both adolescents and caregivers. Construct validity was established through exploratory factor analysis, refining the questionnaire while preserving its original seven dimensions. This validation of the CFMQ highlights its applicability in evaluating family mealtime experiences in this context, providing valuable insights into the dynamics that influence adolescent nutrition and health. Conclusion: The CFMQ proves to be a reliable tool for assessing family mealtime experiences in Mexican adolescents living with obesity and their caregivers who seek care at third-level public hospitals.
Relationship between Fear of COVID-19, Intolerance of Uncertainty, and Coping Strategies on University Students’ Mental Health
Authors: Charbonnier Elodie , Montalescot Lucile , Puechlong Cécile , Goncalves Aurélie , Le Vigouroux Sarah
Published: November 2023

Background: the mental health of students was particularly affected by the COVID-19 pandemic. The present study therefore examined the relationships between anxiety and depressive symptoms, eating-related problems, coping, fear of COVID-19, and intolerance of uncertainty. Methods: 2139 French students of 54 universities were recruited in the different regions of France during a French lockdown (between 21 April and 3 May 2021). Six variables were measured: fear of COVID-19, intolerance of uncertainty, coping, anxiety and depressive symptoms, and eating-related problems. To explore the directions of the relationships between our variables of interest, we calculated a directed acyclic graph. Results: our data highlighted the central roles of intolerance of uncertainty in students’ anxiety and depressive symptoms, and the direct role of prospective intolerance of uncertainty on eating-related problems. Conclusions: these findings indicate that intolerance of uncertainty should be targeted by interventions designed to help students with high levels of anxiety, depressive symptoms, and/or eating-related problems.
Properties of Dietary Flavone Glycosides, Aglycones, and Metabolites on the Catalysis of Human Endoplasmic Reticulum Uridine Diphosphate Glucuronosyltransferase 2B7 (UGT2B7)
Authors: Xu Ting , Lv Yangjun , Cui Yuhan , Liu Dongchen , Xu Tao , Lu Baiyi , Yang Xuan
Published: November 2023

Flavone glycosides, their aglycones, and metabolites are the major phytochemicals in dietary intake. However, there are still many unknowns about the cellular utilization and active sites of these natural products. Uridine diphosphate glucuronosyltransferases (UGTs) in the endoplasmic reticulum have gene polymorphism distribution in the population and widely mediate the absorption and metabolism of endogenous and exogenous compounds by catalyzing the covalent addition of glucuronic acid and various lipophilic chemicals. Firstly, we found that rutin, a typical flavone O-glycoside, has a stronger UGT2B7 binding effect than its metabolites. After testing a larger number of flavonoids with different aglycones, their aglycones, and metabolites, we demonstrated that typical dietary flavone O-glycosides generally have high binding affinities towards UGT2B7 protein, but the flavone C-glycosides and the phenolic acid metabolites of flavones had no significant effect on this. With the disposition of 4-methylumbelliferone examined by HPLC assay, we determined that 10 μM rutin and nicotifiorin could significantly inhibit the activity of recombinant UGT2B7 protein, which is stronger than isovitexin, vitexin, 3-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid. In addition, in vitro experiments showed that in normal and doxorubicin-induced lipid composition, both flavone O-glycosides rutin and flavone C-glycosides isovitexin at 10 μM had no significant effect on the expression of UGT1A1, UGT2B4, UGT2B7, and UGT2B15 genes for 24 h exposure. The obtained results enrich the regulatory properties of dietary flavone glycosides, aglycones, and metabolites towards the catalysis of UGTs and will contribute to the establishment of a precise nutritional intervention system based on lipid bilayers and theories of nutrients on endoplasmic reticulum and mitochondria communication.
A Narrative Review on Strategies for the Reversion of Prediabetes to Normoglycemia: Food Pyramid, Physical Activity, and Self-Monitoring Innovative Glucose Devices
Authors: Rondanelli Mariangela , Barrile Gaetan Claude , Cavioni Alessandro , Donati Paolo , Genovese Elisa , Mansueto Francesca , Mazzola Giuseppe , Patelli Zaira , Pirola Martina , Razza Claudia , Russano Stefano , Sivieri Claudia , Tartara Alice , Valentini Eugenio Marzio , Perna Simone
Published: November 2023

In 2019, “Nutrition Therapy for Adults with Diabetes or Prediabetes: A Consensus Report” was published. This consensus report, however, did not provide an easy way to illustrate to subjects with prediabetes (SwPs) how to follow a correct dietary approach. The purpose of this review is to evaluate current evidence on optimum dietary treatment of SwPs and to provide a food pyramid for this population. The pyramid built shows that everyday consumption should consist of: whole-grain bread or potatoes eaten with their skins (for fiber and magnesium) and low glycemic index carbohydrates (GI < 55%) (three portions); fruit and vegetables (5 portions), in particular, green leafy vegetables (for fiber, magnesium, and polyphenols); EVO oil (almost 8 g); nuts (30 g, in particular, pistachios and almonds); three portions of dairy products (milk/yogurt: 300–400 g/day); mineral water (almost 1, 5 L/day for calcium intake); one glass of wine (125 mL); and three cups of coffee. Weekly portions should include fish (four portions), white meat (two portions), protein plant-based food (four portions), eggs (egg portions), and red/processed meats (once/week). At the top of the pyramid, there are two pennants: a green one means that SwPs need some personalized supplementation (if daily requirements cannot be satisfied through diet, vitamin D, omega-3, and vitamin B supplements), and a red one means there are some foods and factors that are banned (simple sugar, refined carbohydrates, and a sedentary lifestyle). Three to four times a week of aerobic and resistance exercises must be performed for 30–40 min. Finally, self-monitoring innovative salivary glucose devices could contribute to the reversion of prediabetes to normoglycemia.
Effect of a Depolymerase Encoded by Phage168 on a Carbapenem-Resistant Klebsiella pneumoniae and Its Biofilm
Authors: Sun Xu , Pu Bingchun , Qin Jinhong , Xiang Jun
Published: November 2023

Infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are becoming increasingly common within clinical settings, requiring the development of alternative therapies. In this study, we isolated, characterized, and sequenced the genome of a CRKP phage, Phage168. The total genomic DNA of Phage168 was 40,222 bp in length, encoding 49 predicted proteins. Among these proteins, Dep40, the gene product of ORF40, is a putative tail fiber protein that exhibits depolymerase activity based on the result of bioinformatics analyses. In vitro, we confirmed that the molecular weight of the Phage168 depolymerase protein was about 110 kDa, the concentration of the produced phage 168 depolymerase protein was quantified as being 1.2 mg/mL, and the depolymerase activity was still detectable after the dilution of 1.2 µg/mL. This recombinant depolymerase exhibited enzyme activity during the depolymerization of the formed CRKP biofilms. We also found that depolymerase, when combined with polymyxin B, was able to enhance the bactericidal effect of polymyxin B on CRKP strains by disrupting their biofilm. When recombinant depolymerase was used in combination with human serum, it enhanced the sensitivity of the CRKP strain UA168 to human serum, and the synergistic bactericidal effect reached the strongest level when the ratio of depolymerase to human serum was 3:1. Our results indicated that depolymerase encoded by Phage168 may be a promising strategy for combating infections caused by drug-resistant CRKP formed within the biofilm.
Anti-Inflammatory Activity of the Constituents from the Leaves of Perilla frutescens var. acuta
Authors: Youn Isoo , Han Sujin , Jung Hee Jin , Noh Sang Gyun , Chung Hae Young , Koo Yean Kyoung , Shin Sunhye , Seo Eun Kyoung
Published: November 2023

Perilla frutense var. acuta (Lamiaceae) has been used to treat indigestion, asthma, and allergies in traditional medicine. In this study, luteolin 7-O-diglucuronide (1), apigenin 7-O-diglucuronide (2), and rosmarinic acid (3) were isolated from the leaves of P. frutescens var. acuta through various chromatographic purification techniques. Several approaches were used to investigate the anti-inflammatory activity of the constituents (1–3) and their working mechanisms. In silico docking simulation demonstrated that 1–3 would work as a PPAR-α/δ/γ agonist, and in vitro PPAR-α/δ/γ transcriptional assay showed that the Perilla water extract (PWE) and 3 increased PPAR-α luciferase activity (1.71 and 1.61 times of the control (PPAR-α + PPRE, p < 0.001)). In the NF-κB luciferase assay, 1 suppressed NF-κB activity the most (56.83% at 5 µM; 74.96% at 10 µM; 79.86% at 50 µM). In addition, 1 and 2 inhibited the mRNA expression of NF-κB target genes, including Il6, Mcp1, and Tnfa, at 50 µM, and 3 suppressed the genes at the mRNA level in a dose-dependent manner. We report that 1 and 2 exert anti-inflammatory effects through NF-κB inhibition, and the PPAR-α/NF-κB signaling pathway is related to the anti-inflammatory activity of 3.