bluuebunny/crossref_metadata_2025 huggingface dataset:
A Unidirectional DC-DC Autotransformer for DC Grid Application
Authors: Zhou Meng , Xiang Wang , Zuo Wenping , Lin Weixing , Wen Jinyu
Published: March 2018

Conventional unidirectional DC-DC converters for DC grid application employ DC-AC-DC two-stage conversion technology and suffer from high converter cost and power loss. To solve these issues, a unidirectional step-up DC-DC autotransformer (UUDAT) and a unidirectional step-down DC-DC autotransformer (DUDAT) are studied. The UUDAT and DUDAT are composed of a series connection of diode bridges and voltage source converters. Topologies of UUDAT and DUDAT are detailed. The harmonic and un-controllability issues are discussed. Control and possible application scenarios for UUDAT and DUDAT are depicted. DC fault isolation mechanism and the methods of dimensioning the voltage and power ratings of the components in UUDAT and DUDAT are studied. Extensive simulations on power system level and experiments on a UUDAT and DUDAT prototype verified their technical feasibility.
Gardening the City: Addressing Sustainability and Adapting to Global Warming through Urban Agriculture
Authors: Mancebo François
Published: March 2018

This article envisions urban agriculture as a solution to address global warming by decreasing the urban heat island effect while also addressing many other urban sustainability issues, such as multi-functionality, creating new commons, amenities and ecosystem services, reinventing urbanity, encouraging community building by growing local food, and enhanced water management. This article examines how urban design and planning can promote this solution to reconfigure more sustainable and resilient cities. A crucial aspect is that urban planning should evolve from its traditional prescriptive form to adaptive planning. An important point in adaptive planning is that anybody concerned should be associated with the decision-making process, which requires the involvement of citizens in the decisions that affect them.
High Resolution Site Index Prediction in Boreal Forests Using Topographic and Wet Areas Mapping Attributes
Authors: Bjelanovic Ivan , Comeau Philip , White Barry
Published: March 2018

The purpose of this study was to evaluate the relationships between environmental factors and the site index (SI) of trembling aspen, lodgepole pine, and white spruce based on the sampling of temporary sample plots. LiDAR generated digital elevation models (DEM) and wet areas mapping (WAM) provided data at a 1 m resolution for the study area in Alberta. Six different catchment areas (CA), ranging from 0.5 ha to 10 ha, were tested to reveal optimal CA for calculation of the depth-to-water (DTW) index from WAM. Using different modeling methods, species-specific SI models were developed for three datasets: (1) topographic and wet area variables derived from DEM and WAM, (2) only WAM variables, and (3) field measurements of soil and topography. DTW was selected by each statistical method for each species and, in most cases, DTW was the strongest predictor in the model. In addition, differences in strength of relationships were found between species. Models based on remotely-sensed information predicted SI with a root mean squared error (RMSE) of 1.6 m for aspen and lodgepole pine, and 2 m for white spruce. This approach appears to adequately portray the variation in productivity at a fine scale and is potentially applicable to forest growth and yield modeling and silviculture planning.
Dairy Fats and Cardiovascular Disease: Do We Really Need to Be Concerned?
Authors: Lordan Ronan , Tsoupras Alexandros , Mitra Bhaskar , Zabetakis Ioannis
Published: March 2018

Cardiovascular diseases (CVD) remain a major cause of death and morbidity globally and diet plays a crucial role in the disease prevention and pathology. The negative perception of dairy fats stems from the effort to reduce dietary saturated fatty acid (SFA) intake due to their association with increased cholesterol levels upon consumption and the increased risk of CVD development. Institutions that set dietary guidelines have approached dairy products with negative bias and used poor scientific data in the past. As a result, the consumption of dairy products was considered detrimental to our cardiovascular health. In western societies, dietary trends indicate that generally there is a reduction of full-fat dairy product consumption and increased low-fat dairy consumption. However, recent research and meta-analyses have demonstrated the benefits of full-fat dairy consumption, based on higher bioavailability of high-value nutrients and anti-inflammatory properties. In this review, the relationship between dairy consumption, cardiometabolic risk factors and the incidence of cardiovascular diseases are discussed. Functional dairy foods and the health implications of dairy alternatives are also considered. In general, evidence suggests that milk has a neutral effect on cardiovascular outcomes but fermented dairy products, such as yoghurt, kefir and cheese may have a positive or neutral effect. Particular focus is placed on the effects of the lipid content on cardiovascular health.
The Influence of Polymer and Ion Solvation on the Conformational Properties of Flexible Polyelectrolytes
Authors: Chremos Alexandros , Douglas Jack
Published: March 2018

The study of the coupling between the conformational properties of a polyelectrolyte chain and the distribution of counter-ions surrounding the chain is important in developing predictive theories for more complex polymer materials, such as polyelectrolyte gels. We investigated the influence of solvent affinity to counter-ions and the polyelectrolyte backbone on the conformational properties of highly charged flexible polymer chains using molecular dynamics simulations that include both ions and an explicit solvent. We find that the solvation of the polyelectrolyte backbone can be achieved by either increasing the solvent affinity for the polyelectrolyte segments or by increasing the solvent affinity for the counter-ions. However, these two mechanisms influence the conformational properties of the polyelectrolyte chain in rather different ways, suggesting the inadequacy of polyelectrolyte solution models that treat the solvent as a continuum medium.
Wnt Signalling in Intestinal Stem Cells: Lessons from Mice and Flies
Authors: Perochon Jessica , Carroll Lynsey , Cordero Julia
Published: March 2018

Adult stem cells play critical roles in the basal maintenance of tissue integrity, also known as homeostasis, and in tissue regeneration following damage. The highly conserved Wnt signalling pathway is a key regulator of stem cell fate. In the gastrointestinal tract, Wnt signalling activation drives homeostasis and damage-induced repair. Additionally, deregulated Wnt signalling is a common hallmark of age-associated tissue dysfunction and cancer. Studies using mouse and fruit fly models have greatly improved our understanding of the functional contribution of the Wnt signalling pathway in adult intestinal biology. Here, we summarize the latest knowledge acquired from mouse and Drosophila research regarding canonical Wnt signalling and its key functions during stem cell driven intestinal homeostasis, regeneration, ageing and cancer.
The High-Throughput Analyses Era: Are We Ready for the Data Struggle?
Authors: D’Argenio Valeria
Published: March 2018

Recent and rapid technological advances in molecular sciences have dramatically increased the ability to carry out high-throughput studies characterized by big data production. This, in turn, led to the consequent negative effect of highlighting the presence of a gap between data yield and their analysis. Indeed, big data management is becoming an increasingly important aspect of many fields of molecular research including the study of human diseases. Now, the challenge is to identify, within the huge amount of data obtained, that which is of clinical relevance. In this context, issues related to data interpretation, sharing and storage need to be assessed and standardized. Once this is achieved, the integration of data from different -omic approaches will improve the diagnosis, monitoring and therapy of diseases by allowing the identification of novel, potentially actionably biomarkers in view of personalized medicine.
Using Introspection to Collect Provenance in R
Authors: Lerner Barbara , Boose Emery , Perez Luis
Published: March 2018

Data provenance is the history of an item of data from the point of its creation to its present state. It can support science by improving understanding of and confidence in data. RDataTracker is an R package that collects data provenance from R scripts (https://github.com/End-to-end-provenance/RDataTracker). In addition to details on inputs, outputs, and the computing environment collected by most provenance tools, RDataTracker also records a detailed execution trace and intermediate data values. It does this using R’s powerful introspection functions and by parsing R statements prior to sending them to the interpreter so it knows what provenance to collect. The provenance is stored in a specialized graph structure called a Data Derivation Graph, which makes it possible to determine exactly how an output value is computed or how an input value is used. In this paper, we provide details about the provenance RDataTracker collects and the mechanisms used to collect it. We also speculate about how this rich source of information could be used by other tools to help an R programmer gain a deeper understanding of the software used and to support reproducibility.
Spin-Crossover Complexes
Authors: Takahashi Kazuyuki
Published: March 2018

Spin-crossover (SCO) is a spin-state switching phenomenon between a high-spin (HS) and low-spin (LS) electronic configurations in a transition metal center.
Superconducting Strips: A Concept in Thermal Neutron Detection
Authors: Merlo Vittorio
Published: March 2018

In the never-ending quest for better detection efficiency and spatial resolution, various thermal neutron detection schemes have been proposed over the years. Given the presence of some converting layers (typically boron, but 6LiF is also widely used nowadays), the shift towards concepts based on solid state detectors has been steadily increasing and ingenious schemes thereby proposed. However, a trade-off has been always sought for between efficiency and spatial resolution; the problem can be (at least partially) circumvented using more elaborate geometries, but this complicates the sample preparation and detector construction. Thus, viable alternatives must be found. What we proposed (and verified experimentally) is a detection scheme based on the superconducting to normal transition. More precisely, using a boron converting layer, the α particles (generated in the (n, α) reaction) crossing a low critical temperature superconducting strip some 10 µm wide have been detected; the process, bolometric in nature and based on the ionization energy loss, is intrinsically fast and the spatial resolution very appealing. In this work, some of the work done so far will be illustrated, together with the principles of the measurement and various related problems. The realization of the detector is based on industrial deposition and photolitographic techniques well within the grasp of a condensed matter laboratory, so that there is substantial room for improvement over our elementary strip geometry. Some of the plans for future work will also be presented, together with some improvements both in the choice of the materials and the geometry of the detector.
Cruciform Specimen Design for Biaxial Tensile Testing of SMC
Authors: Schemmann Malte , Lang Juliane , Helfrich Anton , Seelig Thomas , Böhlke Thomas
Published: March 2018

This paper presents an investigation of different cruciform specimen designs for the characterization of sheet molding compound (SMC) under biaxial loading. The considered material is a discontinuous glass fiber reinforced thermoset. We define various (material-specific) requirements for an optimal specimen design. One key challenge represents the achievement of a high strain level in the center region of the cruciform specimen in order to observe damage, at the same time prevention of premature failure in the clamped specimen arms. Starting from the ISO norm for sheet metals, we introduce design variations, including two concepts to reinforce the specimens’ arms. An experimental evaluation includes two different loading scenarios, uniaxial tension and equi-biaxial tension. The best fit in terms of the defined optimality criteria, is a specimen manufactured in a layup with unidirectional reinforcing outer layers where a gentle milling process exposed the pure SMC in the center region of the specimen. This specimen performed superior for all considered loading conditions, for instance, in the uniaxial loading scenario, the average strain in the center region reached 87 % of the failure strain in a uniaxial tensile bone specimen.
Prediction and Optimization of Drilling Parameters in Drilling of AISI 304 and AISI 2205 Steels with PVD Monolayer and Multilayer Coated Drills
Authors: Ahmed Yassmin , Youssef Helmi , El-Hofy Hassan , Ahmed Mahmoud
Published: March 2018

Due to their high ductility, high durability, and excellent corrosion resistance, stainless steels are attractive materials for a variety of applications. However, high work hardening, low thermal conductivity, and high built-up edge (BUE) formation make these materials difficult to machine. Rapid tool wear and high cutting forces are the common problems encountered while machining these materials. In the present work, the application of Taguchi optimization methodology has been used to optimize the cutting parameters of the drilling process for machining two stainless steels: austenitic AISI 304 and duplex AISI 2205 under dry conditions. The machining parameters which were chosen to be evaluated in this study are the tool material, cutting speed, and feed rate, while, the response factors to be measured are the tool life (T), cutting force (Fc), and specific cutting energy (ks). Additionally, empirical models were created for predicting the T, Fc and ks using linear regression analysis. The results of this study show that AISI 2205 stainless steel has a shorter tool life, a higher cutting force, and a higher specific cutting energy than AISI 304 stainless steel. In addition, the Taguchi method determined that A3B1C1 and A3B3C1 (A3 = TiN-coated twist drill, B1 = 13 m/min, B3 = 34 m/min, C1 = 0.12 mm/rev) are the optimized combination of levels for the best tool life and the lowest cutting force, respectively. Meanwhile, the optimized combination of levels for all three control factors from the analysis, which provides the lowest specific cutting energy, was found to be A3B1C3 (A3 = TiN-coated twist drill, B1 = 13 m/min, C3 = 0.32 mm/rev) for both stainless steels.
Ocean Thermal Energy Conversion Using Double-Stage Rankine Cycle
Authors: Ikegami Yasuyuki , Yasunaga Takeshi , Morisaki Takafumi
Published: March 2018

Ocean Thermal Energy Conversion (OTEC) using non-azeotropic mixtures such as ammonia/water as working fluid and the multistage cycle has been investigated in order to improve the thermal efficiency of the cycle because of small ocean temperature differences. The performance and effectiveness of the multistage cycle are barely understood. In addition, previous evaluation methods of heat exchange process cannot clearly indicate the influence of the thermophysical characteristics of the working fluid on the power output. Consequently, this study investigated the influence of reduction of the irreversible losses in the heat exchange process on the system performance in double-stage Rankine cycle using pure working fluid. Single Rankine, double-stage Rankine and Kalina cycles were analyzed to ascertain the system characteristics. The simple evaluation method of the temperature difference between the working fluid and the seawater is applied to this analysis. From the results of the parametric performance analysis it can be considered that double-stage Rankine cycle using pure working fluid can reduce the irreversible losses in the heat exchange process as with the Kalina cycle using an ammonia/water mixture. Considering the maximum power efficiency obtained in the study, double-stage Rankine and Kalina cycles can improve the power output by reducing the irreversible losses in the cycle.
Microstructures and Mechanical Properties of Commercially Pure Ti Processed by Rotationally Accelerated Shot Peening
Authors: Huang Zhaowen , Cao Yang , Nie Jinfeng , Zhou Hao , Li Yusheng
Published: March 2018

Gradient structured materials possess good combinations of strength and ductility, rendering the materials attractive in industrial applications. In this research, a surface nanocrystallization (SNC) technique, rotationally accelerated shot peening (RASP), was employed to produce a gradient nanostructured pure Ti with a deformation layer that had a thickness of 2000 μm, which is thicker than those processed by conventional SNC techniques. It is possible to fabricate a gradient structured Ti workpiece without delamination. Moreover, based on the microstructural features, the microstructure of the processed sample can be classified into three regions, from the center to the surface of the RASP-processed sample: (1) a twinning-dominated core region; (2) a “twin intersection”-dominated twin transition region; and (3) the nanostructured region, featuring nanograins. A microhardness gradient was detected from the RASP-processed Ti. The surface hardness was more than twice that of the annealed Ti sample. The RASP-processed Ti sample exhibited a good combination of yield strength and uniform elongation, which may be attributed to the high density of deformation twins and a strong back stress effect.
Regulating the Emission Spectrum of CsPbBr3 from Green to Blue via Controlling the Temperature and Velocity of Microchannel Reactor
Authors: Tang Yong , Lu Hanguang , Rao Longshi , Li Zongtao , Ding Xinrui , Yan Caiman , Yu Binhai
Published: March 2018

The ability to precisely obtain tunable spectrum of lead halide perovskite quantum dots (QDs) is very important for applications, such as in lighting and display. Herein, we report a microchannel reactor method for synthesis of CsPbBr3 QDs with tunable spectrum. By adjusting the temperature and velocity of the microchannel reactor, the emission peaks of CsPbBr3 QDs ranging from 520 nm to 430 nm were obtained, which is wider than that of QDs obtained in a traditional flask without changing halide component. The mechanism of photoluminescence (PL) spectral shift of CsPbBr3 QDs was investigated, the result shows that the supersaturation control enabled by the superior mass and heat transfer performance in the microchannel is the key to achieve the wide range of PL spectrum, with only a change in the setting of the temperature controller required. The wide spectrum of CsPbBr3 QDs can be applied to light-emitting diodes (LEDs), photoelectric sensors, lasers, etc.
Computation of Topological Indices of Some Special Graphs
Authors: Salaheldeen Abdelgader Mohammed , Wang Chunxiang , Abdalrhman Mohammed Sarra
Published: March 2018

There are several chemical indices that have been introduced in theoretical chemistry to measure the properties of molecular topology, such as distance-based topological indices, degree-based topological indices and counting-related topological indices. Among the degree-based topological indices, the atom-bond connectivity ( A B C ) index and geometric–arithmetic ( G A ) index are the most important, because of their chemical significance. Certain physicochemical properties, such as the boiling point, stability and strain energy, of chemical compounds are correlated by these topological indices. In this paper, we study the molecular topological properties of some special graphs. The indices ( A B C ) , ( A B C 4 ) , ( G A ) and ( G A 5 ) of these special graphs are computed.
Mixing Enhancement in Serpentine Micromixers with a Non-Rectangular Cross-Section
Authors: Clark Joshua , Kaufman Miron , Fodor Petru
Published: March 2018

In this numerical study, a new type of serpentine micromixer involving mixing units with a non-rectangular cross-section is investigated. Similar to other serpentine/spiral shaped micromixers, the design exploits the formation of transversal vortices (Dean flows) in pressure-driven systems, associated with the centrifugal forces experienced by the fluid as it is confined to move along curved geometries. In contrast with other previous designs, though, the use of non-rectangular cross-sections that change orientation between mixing units is exploited to control the center of rotation of the transversal flows formed. The associated extensional flows that thus develop between the mixing segments complement the existent rotational flows, leading to a more complex fluid motion. The fluid flow characteristics and associated mixing are determined numerically from computational solutions to Navier–Stokes equations and the concentration-diffusion equation. It is found that the performance of the investigated mixers exceeds that of simple serpentine channels with a more consistent behavior at low and high Reynolds numbers. An analysis of the mixing quality using an entropic mixing index indicates that maximum mixing can be achieved at Reynolds numbers as small as 20 in less than four serpentine mixing units.
A Fluid Inclusion Study of the Brookbank Deposit, Northwestern Ontario: A Transition from Mesothermal to Epithermal Gold Deposition
Authors: Kowalski Barbara , Kissin Stephen
Published: March 2018

The Brookbank and Cherbourg Zone gold deposits are located in the Beardmore–Geraldton greenstone belt of the Wabigoon Subprovince of the Archean Superior Province of the Canadian Shield. Brookbank is a shear zone deposit, whereas the Cherbourg Zone, joined by a shear zone to the Brookbank, is a fissure vein deposit, presumably formed by the same ore-depositing fluid. A total of 375 fluid inclusions, all with low salinity (generally <1 °C freezing point depression) and presence of CO2, from the two deposits were studied. Cross-cutting relationships delineated three stages of vein formation: (1) pre-ore, (2) ore stage and (3) post-ore. Stage 1 inclusions homogenized at ca. 230 °C with low salinity, although at Brookbank a high temperature episode at ca. 360 °C was noted. Stage 2 homogenized at ca. 266 °C at the Cherbourg Zone and over a range 258–269 °C with somewhat higher salinity. Eutectic temperatures indicate that the fluid composition was within the MgCl2-NaCl-H2O system. Fluid immiscibility was prominent in that inclusions homogenized to liquid and vapor at the same temperature were observed. The majority of inclusions from Brookbank homogenized to vapor, whereas those from the Cherbourg Zone homogenized to liquid, marking the transition from mesothermal to epithermal lode gold deposition. Stage 3 fluid inclusions from the Cherbourg Zone homogenized at slightly elevated temperatures and contained fluid possibly representing metastable hydrates of KCl. These post-ore fluids may have been the source of potassium feldspar alteration that overprinted earlier alteration assemblages.
Platy Galena from the Viburnum Trend, Southeast Missouri: Character, Mine Distribution, Paragenetic Position, Trace Element Content, Nature of Twinning, and Conditions of Formation
Authors: Hagni Richard
Published: March 2018

The Viburnum Trend of southeast Missouri is one of the world’s largest producers of lead. The lead occurs as galena, predominantly in two crystallographic forms, octahedrons and cubes. Many studies have shown that octahedral galena is paragentically early, the more abundant of the two crystal forms, and is commonly modified in the cube. Those studies also have shown that the cubic form is paragenetically later, less abundant than the octahedrons, and may exhibit minor octahedral modifications. Viburnum Trend galena crystals that exhibit a platy form have received almost no study. The reason for their lack of study is the rarity of their occurrence. This communication discusses their character, mine distribution, paragenetic position, trace element contents, nature of twinning, and speculated conditions of formation. It also compares their character to similar platy galena occurrences in Germany, Bulgaria, Russia, Mexico, and notes their occurrence at the Pine Point District in the Northwest Territories of Canada and at the Black Cloud mine in Colorado. Flat, platy galena crystals have been recognized to occur in very small amounts in the Magmont, Buick, Fletcher, Brushy Creek, and Sweetwater mines in the Viburnum Trend. In contrast, platy galena has never been observed to occur at the Casteel, West Fork, #27, #28, and #29 mines in the Trend. The platy crystals have formed early in the paragenetic sequence of the ores, prior to and coated by subsequently deposited druzy quartz and cubic galena. Spinel twinning of the octahedron produces flat platy crystals. The platy galena crystals of the Viburnum Trend are very similar in crystal morphology to platy galena crystals interpreted to be spinel twins in the Gonderbach Ag mine in NW Germany, the Dalnegorsk Pb-Zn (skarn deposit) mine in SE Russia, the Madan ore field of skarn Pb-Zn-Ag deposits of southern Bulgaria, and the large Naica skarn Pb mine of northern Mexico. The crystallization of certain crystal forms of galena has been ascribed to the incorporation of elevated contents of trace elements in some lead districts. Analysis of Viburnum platy galena crystals shows that they contain very low levels of trace elements: 3.1 ppm Ag, <2 ppm Bi, <2 ppm Sb, and <2 ppm As. Thus, elevated trace element content is not the cause for the development of Viburnum platy galena. It is speculated that the Viburnum spinel-twinned galena crystals were the result of rapid crystallization from oversaturated hydrothermal ore fluids.
The Multivariate Regression Statistics Strategy to Investigate Content-Effect Correlation of Multiple Components in Traditional Chinese Medicine Based on a Partial Least Squares Method
Authors: Peng Ying , Li Su-ning , Pei Xuexue , Hao Kun
Published: March 2018

Amultivariate regression statisticstrategy was developed to clarify multi-components content-effect correlation ofpanaxginseng saponins extract and predict the pharmacological effect by components content. In example 1, firstly, we compared pharmacological effects between panax ginseng saponins extract and individual saponin combinations. Secondly, we examined the anti-platelet aggregation effect in seven different saponin combinations of ginsenoside Rb1, Rg1, Rh, Rd, Ra3 and notoginsenoside R1. Finally, the correlation between anti-platelet aggregation and the content of multiple components was analyzed by a partial least squares algorithm. In example 2, firstly, 18 common peaks were identified in ten different batches of panax ginseng saponins extracts from different origins. Then, we investigated the anti-myocardial ischemia reperfusion injury effects of the ten different panax ginseng saponins extracts. Finally, the correlation between the fingerprints and the cardioprotective effects was analyzed by a partial least squares algorithm. Both in example 1 and 2, the relationship between the components content and pharmacological effect was modeled well by the partial least squares regression equations. Importantly, the predicted effect curve was close to the observed data of dot marked on the partial least squares regression model. This study has given evidences that themulti-component content is a promising information for predicting the pharmacological effects of traditional Chinese medicine.
Effects of Styrene-Acrylic Sizing on the Mechanical Properties of Carbon Fiber Thermoplastic Towpregs and Their Composites
Authors: Bowman Sean , Jiang Qiuran , Memon Hafeezullah , Qiu Yiping , Liu Wanshuang , Wei Yi
Published: March 2018

Thermoplastic towpregs are convenient and scalable raw materials for the fabrication of continuous fiber-reinforced thermoplastic matrix composites. In this paper, the potential to employ epoxy and styrene-acrylic sizing agents was evaluated for the making of carbon fiber thermoplastic towpregs via a powder-coating method. The protective effects and thermal stability of these sizing agents were investigated by single fiber tensile test and differential scanning calorimetry (DSC) measurement. The results indicate that the epoxy sizing agent provides better protection to carbon fibers, but it cannot be used for thermoplastic towpreg processing due to its poor chemical stability at high temperature. The bending rigidity of the tows and towpregs with two styrene-acrylic sizing agents was measured by cantilever and Kawabata methods. The styrene-acrylic sized towpregs show low torque values, and are suitable for further processing, such as weaving, preforming, and winding. Finally, composite panels were fabricated directly from the towpregs by hot compression molding. Both of the composite panels show superior flexural strength (>400 MPa), flexural modulus (>63 GPa), and interlaminar shear strength (>27 MPa), indicating the applicability of these two styrene-acrylic sizing agents for carbon fiber thermoplastic towpregs.
iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties
Authors: Li Shumei , Su Xueqiang , Jin Qing , Li Guohui , Sun Yanming , Abdullah Muhammad , Cai Yongping , Lin Yi
Published: March 2018

Most pears in Anhui Province are a kind of self-incompatible fruit whose quality is strongly influenced by the male pollen. The proteomic variation of Dangshan Su pollinated by different varieties was analysed using the isobaric tag for relative and absolute quantitation (iTRAQ) to investigate the effect of pollination by different varieties on the pear lignin pathway. Among the 3980 proteins identified from the two samples, 139 proteins were identified as differentially expressed proteins (DEPs). Of these proteins, laccase-4 (LAC4), was found to be related with lignin synthesis, and β-glucosidase 15 (BGLU15) and peroxidase 47 (PER47) were involved in the phenylpropanoid pathway. Moreover, the lignin and stone cell contents were lower in DW (Dangshan Su pollinated by Wonhwang) than those in DJ (Dangshan Su pollinated by Jingbaili). The effect of pollination on the synthesis of lignin through the regulation of the expression of PER47, BGLU15 and LAC4 ultimately affects the formation of stone cells and the fruit quality. We report for the first time that different pollinations influence the protein expression profile in the Dangshan Su pear, and this result provides some new epididymal targets for regulating the synthesis of lignin, regulating the content of stone cells and improving the quality of the pears.
Trimethylamine-N-Oxide (TMAO)-Induced Impairment of Cardiomyocyte Function and the Protective Role of Urolithin B-Glucuronide
Authors: Savi Monia , Bocchi Leonardo , Bresciani Letizia , Falco Angela , Quaini Federico , Mena Pedro , Brighenti Furio , Crozier Alan , Stilli Donatella , Del Rio Daniele
Published: March 2018

One of the most recently proposed candidates as a potential trigger for cardiovascular diseases is trimethylamine-N-oxide (TMAO). Possible direct effects of TMAO on myocardial tissue, independent of vascular damage, have been only partially explored so far. In the present study, we assessed the detrimental direct effects of TMAO on cardiomyocyte contractility and intracellular calcium dynamics, and the ability of urolithin B-glucuronide (Uro B-gluc) in counteracting TMAO-induced cell damage. Cell mechanics and calcium transients were measured, and ultrastructural analysis was performed in ventricular cardiomyocytes isolated from the heart of normal adult rats. Cells were either untreated, exposed to TMAO, or to TMAO and Uro B-gluc. TMAO exposure worsened cardiomyocyte mechanics and intracellular calcium handling, as documented by the decrease in the fraction of shortening (FS) and the maximal rate of shortening and re-lengthening, associated with reduced efficiency in the intracellular calcium removal. Ultrastructurally, TMAO-treated cardiomyocytes also exhibited glycogen accumulation, a higher number of mitochondria and lipofuscin-like pigment deposition, suggesting an altered cellular energetic metabolism and a higher rate of protein oxidative damage, respectively. Uro B-gluc led to a complete recovery of cellular contractility and calcium dynamics, and morphologically to a reduced glycogen accumulation. We demonstrated for the first time a direct negative role of TMAO on cardiomyocyte functional properties and the ability of Uro B-gluc in counteracting these detrimental effects.
Rapid Determination of Active Compounds and Antioxidant Activity of Okra Seeds Using Fourier Transform Near Infrared (FT-NIR) Spectroscopy
Authors: Xia Fangbo , Li Chenchen , Zhao Ning , Li He , Chang Qi , Liu Xinmin , Liao Yonghong , Pan Ruile
Published: March 2018

Okra seeds (OSD) have been proved to possess significantly anti-fatigue activity and due to their high contents of flavonoids and polyphenols. While, the quality of OSD is easily affected by harvest time, region and other factors. In this research, the rapid method based on Fourier transform near infrared (FT-NIR) spectroscopy was developed for quality assessment of okra seeds. Firstly, 120 samples’ spectra were acquired, and quantification of isoquercitrin, quercetin-3-O-gentiobioside, total phenols (TP) and antioxidant assays including 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, ferric reducing antioxidant power (FRAP) were conducted. Next, partial least squares (PLS) regression and full cross-validation were applied to develop calibration models for these data, and external validation was used to determine models’ quality. The coefficient of determination for calibration ( R c 2 ), the root mean square error of cross validation (RMSECV) and the corresponding determination coefficients for cross-validation ( R cv 2 ) proved all these models have excellent precision. Besides, the residual predictive deviation (RPD) of models (4.07 for isoquercitrin, 4.04 for quercetin-3-O-gentiobioside, 9.79 for TP, 4.58 for DPPH and 4.12 for FRAP) also demonstrated that these models possessed good predicative ability. All these results showed that FT-NIR spectroscopy could be used to rapidly determine active compounds and antioxidant activity of okra seeds.
Quantitative 1H-NMR Spectroscopy for Profiling Primary Metabolites in Mulberry Leaves
Authors: Liang Qianqian , Wang Qiuying , Wang Yuan , Wang Ya-nan , Hao Jia , Jiang Miaomiao
Published: March 2018

The primary metabolites in aqueous extract of mulberry (Morus alba L.) leaves were characterized by using proton nuclear magnetic resonance (1H-NMR) spectroscopy. With the convenience of resonance assignment, GABA together with the other 10 primary metabolites was simultaneously identified and quantified in one 1H-NMR spectrum. In this study, external calibration curves for metabolites were employed to calculate the concentrations of interests. The proposed quantitative approach was demonstrated with good linearity (r2 ranged in the interval of 0.9965–0.9999), precision, repeatability, stability (RSD values in the ranges of 0.35–4.89%, 0.77–7.13% and 0.28–2.33%, respectively) and accuracy (recovery rates from 89.2% to 118.5%). The established 1H-NMR method was then successfully applied to quantify 11 primary metabolites in mulberry leaves from different geographical regions within a rapid analysis time and a simple sample preparation procedure.
Enzyme Kinetics and Molecular Docking Studies on Cytochrome 2B6, 2C19, 2E1, and 3A4 Activities by Sauchinone
Authors: Gong Eun , Chea Satya , Balupuri Anand , Kang Nam , Chin Young-Won , Choi Young
Published: March 2018

Sauchinone, an active lignan isolated from the aerial parts of Saururus chinensis (Saururaceae), exhibits anti-inflammatory, anti-obesity, anti-hyperglycemic, and anti-hepatic steatosis effects. As herb–drug interaction (HDI) through cytochrome P450s (CYPs)-mediated metabolism limits clinical application of herbs and drugs in combination, this study sought to explore the enzyme kinetics of sauchinone towards CYP inhibition in in vitro human liver microsomes (HLMs) and in vivo mice studies and computational molecular docking analysis. In in vitro HLMs, sauchinone reversibly inhibited CYP2B6, 2C19, 2E1, and 3A4 activities in non-competitive modes, showing inhibition constant (Ki) values of 14.3, 16.8, 41.7, and 6.84 μM, respectively. Also, sauchinone time-dependently inhibited CYP2B6, 2E1 and 3A4 activities in vitro HLMs. Molecular docking study showed that sauchinone could be bound to a few key amino acid residues in the active site of CYP2B6, 2C19, 2E1, and 3A4. When sibutramine, clopidogrel, or chlorzoxazone was co-administered with sauchinone to mice, the systemic exposure of each drug was increased compared to that without sauchinone, because sauchinone reduced the metabolic clearance of each drug. In conclusion, when sauchinone was co-treated with drugs metabolized via CYP2B6, 2C19, 2E1, or 3A4, sauchinone–drug interactions occurred because sauchinone inhibited the CYP-mediated metabolic activities.
Calixarenes as High Temperature Matrices for Thermally Activated Delayed Fluorescence: C70 in Dihomooxacalix[4]arene
Authors: Palmeira Tiago , Miranda Alexandre , Marcos Paula , Berberan-Santos Mário
Published: March 2018

Thermally activated delayed fluorescence (TADF) of 12C70 and 13C70 was observed up to 140 °C in a p-tert-butyldihomooxacalix[4]arene solid matrix, a temperature range significantly higher than that of previous TADF quantitative studies. An effective singlet–triplet energy gap of 29 kJ/mol and triplet formation quantum yields of 0.97 and 0.99 were measured for 12C70 and 13C70, respectively. The photophysical properties of the two fullerenes in this new matrix are comparable to those obtained in polystyrene at a lower temperature range. Calixarenes are proposed to be suitable matrices for high temperature TADF studies and applications.
Wedelolactone Enhances Osteoblastogenesis through ERK- and JNK-mediated BMP2 Expression and Smad/1/5/8 Phosphorylation
Authors: Zhu Di , Deng Xue , Han Xiao-Fei , Sun Xiao-Xin , Pan Tao-Wen , Zheng Lu-Ping , Liu Yan-Qiu
Published: March 2018

Our previous study showed that wedelolactone, a compound isolated from Ecliptae herba, has the potential to enhance osteoblastogenesis. However, the molecular mechanisms by which wedelolactone promoted osteoblastogenesis from bone marrow mesenchymal stem cells (BMSCs) remain largely unknown. In this study, treatment with wedelolactone (2 μg/mL) for 3, 6, and 9 days resulted in an increase in phosphorylation of extracellular signal-regulated kinases (ERKs), c-Jun N-terminal protein kinase (JNK), and p38. Phosphorylation of mitogen-activated protein kinases (MAPKs), ERK and JNK started to increase on day 3 of treatment, and p38 phosphorylation was increased by day 6 of treatment. Expression of bone morphogenetic protein (BMP2) mRNA and phosphorylation of Smad1/5/8 was enhanced after treatment of cells with wedelolactone for 6 and 9 days. The addition of the JNK inhibitor SP600125, ERK inhibitor PD98059, and p38 inhibitor SB203580 suppressed wedelolactone-induced alkaline-phosphatase activity, bone mineralization, and osteoblastogenesis-related marker genes including Runx2, Bglap, and Sp7. Increased expression of BMP2 mRNA and Smad1/5/8 phosphorylation was blocked by SP600125 and PD98059, but not by SB203580. These results suggested that wedelolactone enhanced osteoblastogenesis through induction of JNK- and ERK-mediated BMP2 expression and Smad1/5/8 phosphorylation.
Lipid-Coated Zinc Oxide Nanoparticles as Innovative ROS-Generators for Photodynamic Therapy in Cancer Cells
Authors: Ancona Andrea , Dumontel Bianca , Garino Nadia , Demarco Benjamin , Chatzitheodoridou Dimitra , Fazzini Walter , Engelke Hanna , Cauda Valentina
Published: March 2018

In the present paper, we use zinc oxide nanoparticles under the excitation of ultraviolet (UV) light for the generation of Reactive Oxygen Species (ROS), with the aim of further using these species for fighting cancer cells in vitro. Owing to the difficulties in obtaining highly dispersed nanoparticles (NPs) in biological media, we propose their coating with a double-lipidic layer and we evaluate their colloidal stability in comparison to the pristine zinc oxide NPs. Then, using Electron Paramagnetic Resonance (EPR) coupled with the spin-trapping technique, we demonstrate and characterize the ability of bare and lipid-coated ZnO NPs to generate ROS in water only when remotely actuated via UV light irradiation. Interestingly, our results reveal that the surface chemistry of the NPs greatly influences the type of photo-generated ROS. Finally, we show that lipid-coated ZnO NPs are effectively internalized inside human epithelial carcinoma cells (HeLa) via a lysosomal pathway and that they can generate ROS inside cancer cells, leading to enhanced cell death. The results are promising for the development of ZnO-based therapeutic systems.
Comparison of Growth of Healthy Term Infants Fed Extensively Hydrolyzed Protein- and Amino Acid-Based Infant Formulas
Authors: Borschel Marlene , Baggs Geraldine , Oliver Jeffery
Published: March 2018

The aim of this narrative review was to assess published growth data for healthy, term, infants consuming extensively hydrolyzed protein-based (EHF), or amino acid-based formulas (AAF). These data may be of use to clinicians managing infants with medical conditions consuming these products. A search was conducted using key terms: amino acid-based, hydrolysate, hydrolyzed, hydrolysed, infant formula, infant formulae or formulas, baby formula, or formulae or formulas, infant, infants, infantile, and growth. Seven controlled, randomized, prospective growth trials of healthy term infants fed EHFs or AAFs at similar time points during the first four months of age met these and other criteria, including that the trial was published in a peer-reviewed journal, subjects were enrolled by ≤14 days of age and were exclusively formula-fed at entry and throughout the duration of the trial, and infants were assessed at regular intervals with weight measures available ideally at 14 days, one, two, three, and four months of age. Results suggested that healthy infants receiving commonly available EHFs and AAFs do not appear to experience accelerated growth as reported for infants fed many standard formulas. Differences in growth patterns were observed with some formulas supporting normative growth patterns during the first four months but others appearing to support markedly lower growth patterns. These observations should be confirmed in well-designed prospective randomized trials. Until that time, it is recommended that EHFs and AAFs be chosen carefully with individual patient needs considered.
Influence of Chitosan Ascorbate Chirality on the Gelation Kinetics and Properties of Silicon-Chitosan-Containing Glycerohydrogels
Authors: Gegel Natalia , Zhuravleva Yulia , Shipovskaya Anna , Malinkina Olga , Zudina Irina
Published: March 2018

The influence of the chirality of chitosan ascorbate on the gelation kinetics and the properties of hybrid silicon-chitosan-containing glycerohydrogels were studied with a deep estimation of the stereospecificity of chitosan polysalts with l- and d-ascorbic acid diastereomers and their biological effects. It has been established that l- and d-diastereomerically enriched chitosan ascorbates are characterized by a positive Cotton effect and differ in the wavelength of the maximum of the dichroic band (250 and 240 nm), as well as in the values of its specific ellipticity (21.8 × 105 and 39.2 × 105 deg·mL·dm−1·g−1), the sign of specific optical rotation (+ and −), the type of dispersion curves (anomalous and smooth), as well as the condensed phase morphology (anisodiametric particles with optical anisotropy and confocal domains of spherical shape, respectively). In the biomimetic sol-gel synthesis of silicon-chitosan-containing glycerohydrogels using silicon tetraglycerolate as a precursor, it was found that chitosan d-ascorbate retarded gelation. Thin congruent plates obtained from the corresponding glycerohydrogels based on chitosan d-ascorbate have higher mechanical strength and elasticity under uniaxial stretching and lower values of Young’s modulus. It has been shown that the systems based on chitosan d-ascorbate show the greatest antibacterial activity against Staphylococcus aureus 209P and Escherichia coli 113-13 and significantly promote the viability of normal human dermal fibroblasts. The results of our assessment of the biological properties of chitosan polysalts are unexpected, since ascorbic acid exhibits biological activity as its l-isomer only.
Humidity Induces Changes in the Dimensions of Hydrogel-Coated Wool Yarns
Authors: Wang Lanlan , Cavaco-Paulo Artur , Xu Bo , Martins Madalena
Published: March 2018

Polymeric hydrogel based on acrylic acid (AA) and N,N-dimethylacrylamide (DMAA) was prepared by photopolymerization reaction, using nano-alumina as the inorganic crosslinker. Hydrogel-coated wool yarns determine their dimensional changes under humidity conditions. Surface morphology of the hydrogel-coated wool yarns was carried out using SEM microscopy. The hydrogel was further characterized by Fourier transformer infrared spectrum (FTIR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), thermogravimetry (TG) and differential thermogravimetry (DTG). This contribution showed that UV-initiated polymerization coating wool yarns can change the functional properties of wool fibers.
Bleaching of Neutral Cotton Seed Oil Using Organic Activated Carbon in a Batch System: Kinetics and Adsorption Isotherms
Authors: Chetima Abba , Wahabou Abdoul , Zomegni Gaston , Ntieche Rahman Abdoul , Bup Nde Divine
Published: March 2018

In the processing of cotton and neem seeds to obtain oil for diverse uses, enormous quantities of seed husk are generated as waste, which when not properly disposed of, poses environmental problems. One way of reducing this waste is to use it for the production of activated carbon (AC) for its multiple applications. In this work, activated carbon was produced from cotton and neem seed husks by carbonization followed by acid activation. The prepared ACs were characterized for its porosity and surface properties as well as for its ability to bleach neutral cotton seed oil. The prepared ACs are very efficient in the decoloration process, as they removed about 96–98% of the pigments compared to 98.4% removal with commercial bleaching earth. Temperature had a pronounced effect on the bleaching of neutral cotton seed oil. Maximum adsorption was observed at 60 °C for a contact time of 45 min. The adsorption kinetics were modelled by the intra-particle and the pseudo-second order equations while the adsorption isotherms followed the Langmuir and Freundlich equations. It is concluded that the organic ACs are efficient in pigment removal from neutral cotton seed oil and therefore are potential bleaching agents for the vegetable oil industry.
Fuel Gas Network Synthesis Using Block Superstructure
Authors: Li Jianping , Demirel Salih , Hasan M.
Published: March 2018

Fuel gas network (FGN) synthesis is a systematic method for reducing fresh fuel consumption in a chemical plant. In this work, we address FGN synthesis problems using a block superstructure representation that was originally proposed for process design and intensification. The blocks interact with each other through direct flows that connect a block with its adjacent blocks and through jump flows that connect a block with all nonadjacent blocks. The blocks with external feed streams are viewed as fuel sources and the blocks with product streams are regarded as fuel sinks. An additional layer of blocks are added as pools when there exists intermediate operations among source and sink blocks. These blocks can be arranged in a I × J two-dimensional grid with I = 1 for problems without pools, or I = 2 for problems with pools. J is determined by the maximum number of pools/sinks. With this representation, we formulate FGN synthesis problem as a mixed-integer nonlinear (MINLP) formulation to optimally design a fuel gas network with minimal total annual cost. We revisit a literature case study on LNG plants to demonstrate the capability of the proposed approach.