bluuebunny/crossref_metadata_2025 huggingface dataset:
EVALUASI ON GOING DANA ALOKASI KHUSUS FISIK BIDANG PENDIDIKAN DALAM MASA PANDEMI CORONA VIRUS DISEASE-19 BERBASIS INTELLIGENCE MEDIA MANAGEMENT (IMM)
Authors: Pambudi Andi Setyo
Published: January 2022

Alokasi DAK bidang pendidikan selalu tersedia setiap tahun dengan jumlah anggaran yang besar dibandingkan bidang yang lain, bahkan di masa pandemi COVID-19. Pada level pemerintah daerah, bidang pendidikan adalah prioritas. Kebutuhan pendanaan pemerintah daerah melalui DAK Fisik diharapkan selaras dengan keinginan pemerintah pusat. Pelaksanaan DAK Fisik Bidang Pendidikan sebagai instrumen pendanaan ke daerah pada tahun 2020 menghadapi tantangan mewabahnya COVID-19 pada bulan Maret yang mempengaruhi optimalisasi proses pembangunan. Pemerintah mengambil opsi kebijakan relaksasi untuk mempermudah implementasi DAK di lapangan. Hal ini memerlukan evaluasi on-going dengan metode sesuai keadaan saat itu sebagai masukan untuk perencanaan tahun berikutnya. Implementasi DAK Fisik dan segala menu-menunya mendorong pentingnya informasi capaian maupun kendala lapangan tetap aktual serta dapat dipertanggungjawabkan secara ilmiah. Penelitian bertujuan menyajikan kendala, informasi dan hal-hal terkait pelaksanaan DAK Fisik Bidang Pendidikan tahun 2020 saat masa pandemi COVID-19 sebagai respons kondisi terkini tanpa harus melakukan kunjungan lapangan. Metode yang digunakan adalah studi literatur dan analisis statistik berbasis big data sebagai opsi yang relevan dengan situasi saat ini dalam rangka landasan memberikan rekomendasi perencanaan pembangunan. Analisis big data memanfaatkan tools Intelligence Media Management (IMM) untuk melihat persepsi publik terkait pelaksanaan DAK Fisik Bidang Pendidikan saat pandemi COVID-19. Hasil penelitian menunjukkan bahwa sebanyak 40 persen pemberitaan terkait DAK Bidang Pendidikan selama Pandemi COVID-19 berupa sentimen positif, 17 persen negatif dan 43 persen sentimen netral. Dalam sentimen negatif, pemberitaan yang disorot adalah tuntutan agar pengelola DAK dapat segera menyelesaikan pembangunan sesuai petunjuk teknis di lapangan.  Top issue pemberitaan DAK Pendidikan sebagai masukan perbaikan pembangunan adalah tentang temuan-temuan ketidaksesuaian data pokok pendidikan (DAPODIK) dengan fakta di lapangan yang pada akhirnya berdampak pada kurang tepatnya sasaran penerima DAK.

Kata Kunci: Big Data, DAK, Evaluasi, Pendidikan
PERAN APARATUR DALAM MENGATASI PENULARAN PANDEMI COVID-19 DI KABUPATEN BANDUNG BARAT PROVINSI JAWA BARAT
Authors: Mulianingsih Sunasih
Published: January 2022

Abstrak

Maksud dan tujuan dari penelitian ini adalah untuk mendiskripsikan dan  mendapatkan data, sejauhmana peran aparatur Kabupaten Bandung Barat dalam menangani pendemi covid 19, Kabupaten Bandung Barat saat ini termasuk wilayah yang terdampak penyebaran dan penularan covid 19, dimana sebahagian Masyarakat dan jajaran aparatur terkonfimasi positif terkena covid 19. Sehingga peneliti ingin mengetahui sejauhmana peran aparatur Kabupaten Bandung Barat dalam mengatasi penularan pandemi covid 19. Penelitian ini menggunakan metode kualitatif diskriptif dengan pendekatan induktif, dan dapat diperoleh melalui data sekunder yang bersumber dari media cetak media elektronik dan dokumentasi-dokumentasi terkait dengan covid 19. Simpulan bahwa dalam implementasinya peran aparatur sangat besar dalam menangani msibah kesehatan ini, tim gugus tugas yang dibentuk oleh Bupati, mengawasi jalannya operasional sehari-hari. Hasil nya Kabupaten Bandung Barat dapat mengendalikan angka penularan dan yang sembuh semakin meningkat. Walaupun saat ini covid 19 belum melandai, tetapi khususnya di Kabupaten Bandung Barat dapat dikendalikan.

 

Kata Kunci : Peran, Aparatur, Penularan dan Covid 19.

 

Abstract

The purpose and objective of this study is to describe and obtain data, to what extent is the role of the West Bandung Regency apparatus in dealing with the COVID-19 pandemic, currently West Bandung Regency is an area affected by the spread and transmission of COVID-19, where some of the community and apparatus have been confirmed positive for COVID-19. 19. So that researchers want to know the extent of the role of the West Bandung Regency apparatus in overcoming the transmission of the covid 19 pandemic. This study uses descriptive qualitative methods with an inductive approach, and can be obtained through secondary data sourced from print media, electronic media and documentation related to covid 19. The conclusion is that in its implementation the role of the apparatus is very large in handling this health problem, the task force team formed by the Regent oversees the day-to-day operations. As a result, West Bandung Regency was able to control the number of infections and those who recovered were increasing. Even though at this time Covid 19 has not been sloping, but especially in West Bandung Regency, it can be controlled.

 

Keywords: Role, Apparatur, Transmission and Covid 19
Analisis Dampak Pengembangan Karir PNS Pasca Pelaksanaan Alih Jabatan Struktural ke Jabatan Fungsional
Authors: Marthalina Marthalina
Published: January 2022

Penyederhanaan birokrasi berupa alih jabatan struktural ke jabatan fungsional pada Kementerian PAN RB bertujuan menciptakan iklim birokrasi yang lincah, dinamis dan profesional dalam rangka meningkatkan efektivitas dan efisiensi kinerja pemerintahan dalam pelayanan publik. Dalam pelaksanaannya, ada beberapa penyesuaian yang harus dihadapi oleh ASN dan organisasi. Tujuan penelitian adalah untuk memotret dampak dari implementasi perampingan birokrasi ini terhadap perkembangan karir ASN pasca pergantian jabatan. Subjek penelitian ini adalah ASN yang melaksanakan pengalihan jabatan administrator atau eselon III dan jabatan pengawas atau eselon IV menjadi jabatan fungsional. Metode penelitian menggunakan analisis deskriptif melalui tahap orientasi, tahap eksplorasi dan akhirnya tahap pemeriksaan anggota dengan sumber daya penelitian menggunakan orang, kertas dan tempat. Akibatnya, pelaksanaan alih jabatan struktural ke jabatan fungsional tidak berdampak langsung pada perkembangan karier ASN di organisasi Kementerian PAN RB selama setahun terakhir. Hal ini dikarenakan beberapa tantangan perubahan yang masih perlu disesuaikan oleh ASN dan organisasi Kementerian PAN RB. Meskipun secara konseptual pola pengembangan karir ASN umum telah dibuat, tetapi tidak semua pola pengembangan karir jabatan fungsional untuk jenis jabatan tersedia. Hal lain yang menjadi pertimbangan untuk pengembangan karier di jabatan fungsional adalah desain organisasi yang diharapkan gesit atau lincah yang akan mempengaruhi bagaimana perkembangan karier ASN di Kementerian PAN RB ke depan.
Pengaruh Kinerja Aparatur Terhadap Kualitas Pelayanan Pembuatan Kartu Keluarga di Kecamatan Tambun Utara Kabupaten Bekasi
Authors: Rachman Muhammad Widigdo , Kurniansyah Dadan , Ramdani Rachmat , Hapsari Rian
Published: January 2022

This study aims to determine the effect of apparatus performance on the service quality of making family cards in Tambun Utara district, Bekasi district. This research is a quantitative research through survey method with correlation regression data analysis technique. Using the Performance questionnaire instrument to measure the performance of the apparatus includes 4 aspects, namely: quality, quantity, use of time, cooperation and the service quality questionnaire covers 5 aspects, namely: physical form (responsiable), reliability (reability), responsiveness (responsiviness), assurance (assurance. ) and empathy (emphaty). The sample consisted of 122 family heads who took care of making family cards in North Tambun District in 2019-2010. The results showed that there is a significant influence between the performance of the apparatus on the quality of service for making family cards in Tambun Utara District, Bekasi Regency, which is equal to 52.8%, the remaining 47.2% is influenced by other factors outside the performance of the apparatus. Based on the results of the above research, it is concluded that there is a strong influence between the performance of the apparatus on the quality of service for making family cards in Tambun Utara District, Bekasi Regency.
ANALISIS JABATAN DALAM PENEMPATAN PEGAWAI DI BIRO PEMERINTAHAN DAN OTONOMI DAERAH SEKRETARIAT PROVINSI SULAWESI UTARA
Authors: Tamara Claudio V.D. , Johanes Ayu Widowati , Marlina Lina , Hendra Khansa Aulia
Published: January 2022

Abstrak

 

Dalam penyelenggaraan manajemen sumber daya pegawai sektor publik, terdapat bahasan yang menarik untuk dikaji yaitu tentang penempatan pegawai berdasarkan kompetensinya sehingga dapat melakukan pekerjaaannya dengan efektif. Penelitian berfokus untuk untuk mengkaji dan menjelaskan analisis jabatan dalam menempatkan pegawai di Biro Pemerintahan Dan Otonomi Daerah, Sekretariat Provinsi Sulut. Penelitian ini menggunakan metode deskritif kualitatif. Data diperoleh dari objek penelitian yang terdiri dari para pejabat struktural, fungsional dan staf biro pemerintahan dan otonomi daerah pada pemerintah Provinsi Sulut. Analisis jabatan pada Biro Pemerintahan dan Otonomi Daerah di Pemerintah Provinsi Sulut telah dilaksanakan namun belum maksimal hal ini dapat dilihat masih banyak posisi jabatan yang kosong (belum terisi) dan ada beberapa ketidaksesuaian antara pendidikan pemangku jabatan dan klasifikasi jabatan.

 

Kata Kunci: Analisis Jabatan, Penempatan Pegawai, Pengembangan Kompetensi
EVALUASI KEBIJAKAN PROVINSI SUMATERA BARAT TENTANG PENDIRIAN BUMD PT. BALAIRUNG CITRAJAYA SUMBAR
Authors: Putra Hadi , Koeswara Hendri , Syamsurizaldi Syamsurizaldi
Published: January 2022

Salah satu bentuk kebijakan publik pemerintah daerah yaitu kebijakan Pendirian Badan Usaha Milik Daerah. Tujuan penelitian ini adalah mengevaluasi kebijakan Pendirian Badan Usaha Milik Daerah PT. Balairung Citrajaya Sumbar.Teori yang digunakan adalah evaluasi kebijakan Althaus et al. dengan indikator: input, proses, output, dan outcome. Pendekatan penelitian yang digunakan deskriptif kualitatif dengan pengumpulan data telaah dokumen, wawancara dan observasi. Hasil penelitian menunjukkan bahwa kebijakan pendirian PT. Balairung Citrajaya Sumbar belum efektif dan tidak efisien berdasarkan; (1) Input, bahan dasar kebijakan tidak terdapat studi kelayakan, sumber daya manusia sudah memadai, sumberdaya keuangan belum seluruh penyertaan modal diterima dari kabupaten/kota sesuai perjanjian kerjasama. (2) Proses, pengimplementasian kebijakan sudah mencapai sasaran yaitu terbentuk dan beroperasinya PT. Balairung Citrajaya Sumbar. Implementor kebijakan sudah menjalankan peran sesuai fungsi masing-masing. Namun belum efektif dalam pencapaian tujuan kebijakan serta tidak efisien mengelola sumberdaya menjadi output,(3) Output, hasil kebijakan sudah diterima berupa deviden, namun rasionya masih sangat kecil dibandingkan input dan belum diterima secara kontiniu setiap tahun. (4) Outcome, tujuan formal kebijakan untuk peningkatan pembangunan masih belum signifikan karena kecilnya kontribusi untuk PAD. Dampak positif lainnya yaitu secara sosial budaya Hotel Balairung menjadi icon dan meeting point masyarakat Sumatera Barat di Jakarta. Sedangkan dampak negatifnya antara lain image Pemerintah Provinsi Sumatera Barat tidak mampu mengelola BUMD dengan baik dan penambahan beban APBD untuk sewa kantor Badan Penghubung Provinsi Sumatera Barat di Jakarta, serta berkurangnya fungsi Balairung sebagai tempat bernaungnya orang Minang di Jakarta.
Upaya Badan Kepegawaian Daerah Mengimplementasikan Kebijakan Disiplin ASN dalam Mengelola Keuangan Daerah di Kabupaten Agam
Authors: Dal Zaini Juniansyah
Published: January 2022

Penelitian ini dilatarbelakangi oleh rendahnya tingkat disiplin Pegawai Negeri Sipil (PNS). Penelitian ini dengan tujuan guna meningkatkan pemahaman betapa berartinya ketertiban untuk Aparatur Sipil Negara selaku penyelenggaraan pemerintahan dalam rangka mewujudkan pemerintahan yang baik sebab tegak ataupun runtuhnya negara bergantung dari sumber daya manusianya ialah Aparatur Negara. Guna riset ini diharapkan bisa membagikan kontribusi dalam pengembangan pemberdayaan disiplin ASN selaku aparatur pemerintah, abdi negara serta abdi masyarakat jua bisa membagikan kontribusi pemikiran serta wacana dalam penyelenggaraan pemerintahan yang bersih serta berwibawa khususnya dalam perihal penerapan peraturan kepegawaian untuk ASN. Teknik pengumpulan data dilakukan melalui metode studi pustaka dan studi dokumen serta terjun langsung kelapangan dimana  yang diteliti berupa aturan-aturan yang tertulis dalam undang-undang yang berlaku, dengan pengamatan secara langsung melalui wawancara terhadap responden. Hasil penelitian ini terbukti kalau masih adanya pelanggaran disiplin yang dilakukan oleh ASN yang mempengaruhi citra reformasi birokrasi dinegara Indonesia. Upaya untuk meningkatkan disiplin  pegawai akan terus dilakukan sehingga ASN bisa lebih baik kedepannya.
İslam Hukukunda Yaşlı Bakım Mükellefiyeti
Authors: Atalay Algan Nazan
Published: January 2022

Yaşam süresinin uzadığı ve demografik yapının yaşlılar lehine tavana doğru genişleme gösterdiği günümüzde yaşlı nüfus sayıca kalabalık bir kesimi oluşturmaktadır. Buna karşın üretici-genç nüfusun azalması, bakımda aktif rol oynayan kadınların çalışma hayatına girerek evden uzaklaşmaları, bireysel yaşam tarzının yaygınlaşarak dinî ve ahlâkî değerlerden uzaklaşılması ve modernleşme gibi faktörler günümüzde ve gelecekte kitlesel bir tehdit oluşturan bakım meselesinin yeniden ele alınmasını gerekli kılmıştır. 20. asrın ikinci yarısından itibaren yaşlı bakımı meselesi pek çok kesimin dikkatini çekmiş ve farklı bilim dalları tarafından çeşitli açılardan incelenmeye çalışılmıştır. Konunun dinî bir mükellefiyeti de içermesi nedeniyle meselenin İslam hukuku açısından incelenmesi uygun görülmüş ve konuyla alakalı bir nebze de olsa alana katkı sunulmak istenmiştir. Bu çerçevede İslam hukukuna göre yaşlıların bakılma hakkının olup olmadığı, varsa bu haklara dayanak teşkil eden esasların ve bakımla sorumlu olan kimselerin tespiti amaçlanmıştır. Araştırma sonucunda hayatın her aşamasında insanın bakım ve gözetiminin emredildiği ve yaşlı bakım mükellefiyetinin kişinin kendisinden başlayarak tüm toplumu ve devleti içine alacak şekilde genişlediği sonucuna varılmıştır.
PEMANFAATAN MEDIA PEMBELAJARAN DARING DALAM PROSES BELAJAR MENGAJAR
Authors: Wiradharma Gunawan , Ruliana Poppy , Prisanto Guntur Freddy
Published: January 2022

Pandemi Covid-19 telah berdampak ke semua aspek kehidupan, seperti bidang pendidikan. Penyelenggaraan perkuliahan pada tahun 2020 dilakukan secara daring sehingga memerlukan media pembelajaran daring, baik bersifat sinkronus maupun asinkronus sesuai kebijakan Kementerian Pendidikan dan Kebudayaaan. Tujuan penelitian ini adalah untuk mengetahui dan menganalisis persepsi pemanfaatan media pembelajaran daring yang digunakan dalam proses belajar mengajar selama pandemi. Teori yang digunakan dalam penelitian ini adalah Technology Acceptance Model dari Davis. Penelitian ini dilakukan dengan survei kepada 225 mahasiswa semester 3 tahun ajaran 2020/2021. Metode penelitian menggunakan pendekatan kuantitatif dengan melakukan survei online dengan menggunakan pengisian kuesioner melalui google form terhadap responden yang merupakan mahasiswa program studi ilmu komunikasi Universitas Pakuan, Bogor. Analisis data menggunakan analisis deskriptif dan structural equation model (SEM) partial least square (PLS). Hasil penelitian ini menunjukkan bahwa terdapat pengaruh nyata variabel persepsi kegunaan menggunakan teknologi (perceived usefulness) terhadap variabel persepsi kemudahan dalam menggunakan teknologi (perceived ease of use); terdapat pengaruh nyata persepsi kemudahan dalam menggunakan teknologi (perceived ease of use) terhadap variabel sikap (attitude); dan terdapat pengaruh nyata variabel sikap (attitude) terhadap penerimaan media (intention to use).
Strategi Komunikasi Krisis Pengelola Desa Wisata Dam Jati Dalam Menghadapi Pandemi Covid-19
Authors: Putri Rizqi Nur Arieska , Andhita Pundra Rengga
Published: January 2022

Dampak destruktif dari pandemi covid-19 telah menyentuh berbagai sektor kehidupan manusia, salah satunya adalah sektor pariwisata. Hal inilah yang juga dialami oleh pengelola Wisata Dam Jati yang terletak di desa Goranggareng, Nguntoronadi, Kabupaten Magetan. Krisis yang diakibatkan pandemi covid-19 berpotensi besar berdampak pada krisis fiannasia yang akan berimplikasi juga pad akesejahteraan masyarakat skeitar. Penelitian ini menggunakan metode deskriptif kualitatif dengan teknik pengumpulan data yang menitikberatkan pada wawancara mendalam, observasi dan studi literasi. Penelitian ini menitikberatkan pada strategi komunikasi krisis yang dilakukan pengelola Wisata Dam Jati dalam menghadapi pandemi covid-19 dengan pendekatan Situational Crisis Communication Theory (SCCT). Hasil penelitian menunjukkan bahwa dalam menyikpai krisis, pengelola tempat Wisata Dam Jati melakukan pendekatan SCCT yang diawali dengan pemetaan terhadap jenis krisis, penyusunan strategi melalui justification dan compensation hingga pada soliditas pengelolaan internal organisasi. Melalui penelusuran terhadap ketiganya pengelola Wisata Dam Jati berhasil menuai hasil positif. Ini terlihat dari adanya kenaikan jumlah pengunjung dalam rentang waktu tertentu. Kenaikan itu juga yang berimplikasi pada pendapatan finansial organisasi. Dengan adanya pendapatan finansial, pengelola Wisata Dam Jati berhasil mencegah potensi destruktif lain yang diakibatkan pandemi covid-19. Namun untuk menjaga simultansi ketahanan organisasi dalam menghadapi krisis, pengelola sudah harus mulai membangun komunikasi yang intensif dan masif dengan berbagai pihak di luar organisasi seperti pemerintah, swasta dan media massa. Ini penting karena hasil akhir dari pandemi covid-19 belum bisa diprediksi dengan tepat. Pelibatan berbagai pihak dapat meringankan dampak krisis yang dialami oleh organsasi.
DINAMIKA NEGOSIASI ORGANISASI HYBRID KEWIRAUSAHAAN SOSIAL AGRADAYA DALAM MELAKUKAN AKTIVITAS COMMUNITY DEVELOPMENT
Authors: Praharsiwi Caecilia Santi
Published: January 2022

Tulisan ini berupaya mengkaji dinamika social enterprise (SE) sebagai organisasi hybrid dalam bernegosiasi dengan komunitas yang dibina. AGRADAYA yang menjadi subjek dalam penelitian ini masuk dalam kategori SE dimana setiap proses produksi barangnya bergantung pada relasi mereka dengan komunitas petani rempah yang dibina menjadi supplier. Model bisnis ini membuat relasi SE dengan komunitasnya bukan hanya berwujud filantropis, melainkan juga bercampur dengan bisnis. Organisasi hybrid yaitu SE dengan misi profit dan non-profit mengalami dinamika yang unik ketika dalam melaksanakan program pendampingan kepada komunitas petani, harus menghadapi dinamika negosiasi demi mendapatkan sumber daya rempah kering yang dibutuhkan. Penelitian ini menemukan bahwa dinamika negosiasi yang muncul kemudian dipengaruhi oleh posisi komunitas petani sebagai stakeholder dengan atribut dominant yang memiliki power dan legitimacy sehingga Agradaya harus hati-hati agar relasinya tetap terjaga. Meskipun SE berusaha menerapkan kesetaraan dalam menjalin relasinya, pemberdayaan komunitas masih menjadi tantangan besar bagi organisasi hybrid untuk terus berjalan dengan misi profit, people, and planet.
KONTEN MEDIA EDUKASI ANAK BERBASIS SELF CONCEPT THEORY
Authors: Pratiwi Mutia Rahmi , Hening Y. Dzuha
Published: January 2022

Anak-anak memiliki masa emas atau golden age yang idealnya merupakan masa tumbuh optimal anak dengan dukungan adaptasi lingkungan yang baik. Pada kenyataannya jumlah angka kekerasan seksual pada anak di Indonesia terus meningkat mencapai lebih dari 600 kasus di tahun 2019. Berbagai langkah preventif telah dilakukan dari sisi pemerintah hingga di tataran akademisi melalui ragam media edukasi sebagai media pembelajaran pada anak akan bahaya tindak kekerasan anak. Penelitian ini bertujuan untuk menganalisis media edukasi terkait perlindungan anak yang telah dibuat oleh peneli sebelumnya berbasis pada self concept theory. Jenis penelitian ini adalah kualitatif deskriptif dengan metode analisis isi. Hasil penelitian menunjukan bahwa pada media edukasi sebelumnya baik berupa teks maupun audio visual, mengedepankan dimensi internal yaitu behavioral self dan judging self. Pada dimensi eksternal, muncul keberagaman esensi utama media edukasi yang sudah dipublikasikan, yaitu: ethical moral self, physical self, family self dan social self. Maka dapat disimpulkan bahwa dimensi self yang tidak ditampilkan dalam media edukasi sebelumnya adalah self identity dan personal self. Temuan ini akan menjadi dasar akan dibentuknya perancangan media edukasi anak yang konsep utamanya adalah mengajarkan anak untuk memperkuat konsep dirinya sehingga menjadi fondasi kuat bagi anak dalam melindungi diri dari berbagai tindak kekerasan.
PENGUATAN PUBLIK DALAM PEMBAHASAN RANCANGAN UNDANG-UNDANG PENYIARAN
Authors: Herman Herman herman
Published: January 2022

Pembahasan Rancangan Undang-Undang Nomor. 32/2002 mengenai penyiaran berlangsung dari 2017 hingga saat ini, DPR sebagai pengusul berkomitmen RUU tersebut mutlak disahkan periode DPR 2019-2024. Berlarut-larutnya pembahasan terletak pada tata cara perpindahan dari analog ke digital, dimana masing-masing fraksi memiliki kepentingan. Selain itu DPR juga berpegang pada Undang-Undang Nomor 12 Tahun 2011 terkait Pembentukan Peraturan Perundang-Undangan, sehingga perdebatan antara kepentingan dan peraturan perundang-undangan di DPR menjadi faktor yang sulit disatukan. Melalui studi kualitatif ke lapangan didapatkan bahwa berlarut-larutnya pembahasan RUU terletak pada regulasi di DPR dan perbedaan pandangan terkait penyelenggara multiplexing. Melalui Teori Strukturasi Anthony Giddens dan stategi penelitian yang ditetapkan dapat dilihat peran struktur dan agen dalam pembentukan RUU tersebut, dimana strukturasi dapat dibaca bahwa tindakan-tindakan sosial itu selalu bergulir dari waktu ke waktu, kapanpun dan dimanapun, sehingga menimbulkan perubahan-perubahan sosial dan tindakan sosial, hal tersebut dapat diamati dari dua konsep besar, yakni agen dan struktur. Agen bisa individu bisa institusi bisa apapun, sedangkan struktur terdiri dari komponen rule dan resources
PENGARUH PEMBERITAAN KASUS TUDUHAN EKSPLOITASI ANAK TERHADAP CITRA PT DJARUM INDONESIA
Authors: Maulida Rizkiya Ayu , Uljanatunnisa Uljanatunnisa , Sevilla Vinta
Published: January 2022

Perseteruan antara KPAI (Komisi Perlindungan Anak Indonesia) dengan Djarum Foundation mengenai tuduhan eksploitasi anak pada audisi PB Djarum telah memberikan publisitas yang kurang baik kepada PT Djarum. Terlebih, kegiatan tanggung jawab sosial perusahaan pada perusahaan rokok sering dipandang hanya sebagai upaya semata untuk menutupi kerugian yang disebabkan oleh produk rokok. Publisitas merupakan salah satu alat untuk membangun citra dari perusahaan. Kegiatan publisitas dapat dikatakan adalah upaya untuk membentuk citra melalui dukungan pihaak ketiga, yaitu media massa. Akan tetapi, pada kasus ini, publisitas yang muncul adalah justru berasal dari berita yang kurang baik mengenai perusahaan. Pada penelitian ini, tim peneliti ingin menggali pengaruh publisitas yang diperoleh dari konflik antara KPAI (Komisi Perlindungan Anak Indonesia) dan Djarum Foundation terhadap citra PT Djarum sebagai perusahaan rokok. Penelitian ini merupakan penelitian kuantitatif dengan metode surveI. Responden dari penelitian ini adalah mahasiswa S1 Prodi Ilmu Komunikasi, UPN Veteran Jakarta, angkatan 2017-2019. Hasil penelitian menunjukkan bahwa pemberitaan mengenai perseteruan KPAI dengan Djarum justru memberikan pengaruh positif pada citra yang dibangun oleh PT Djarum. Hal ini menunjukkan bahwa publisitas yang kurang baik mengenai perusahaan tidak selalu memberikan dampak negatif terhadap citra perusahaan.
Peran Media Massa dalam Mendukung Upaya Diplomasi Indonesia terhadap Isu Perbatasan dengan Cina
Authors: Kusumajanti Kusumajanti , Nashier Asep Kamaluddin
Published: January 2022

Kepulauan Natura yang berada di wilayah Kabupaten Natuna, Provinsi Kepulauan Riau masih menjadi menjadi perbincangan dunia dikrenakan Cina mengklaim secara sepihak sebagian wilayah Natuna merupakan kedaulatannya. Penelitian ini bertujuan untuk melihat peran media massa dalam mendukung upaya diplomasi Indonesia terhadap Isu Perbatasan dengan Cina. Wilayah perbatasan Indonesia di Laut Natuna Utara belakangan ini menjadi perbincangan dunia. Peristiwa masuknya kapal-kapal Cina ke Zona Ekonomi Ekslusif (ZEE) di Laut Natuna Utara menjadi peringatan bahwa penguatan kapasitas pemerintah kabupaten Natuna guna menghadapi tantangan di wilayah perbatasan. Media Nasional maupun internasional banyak memberitakan tentang permasalahan ini. Framing yang dilakukan oleh media-media Indonesia secara online dapat menjadi salah satu upaya untuk memperkuat diplomasi perbatasan yang dilakukan oleh pemerintah daerah maupun pemerintah pusat.
Early Phenotype Programming in Birds by Temperature and Nutrition: A Mini-Review
Authors: Andrieux Charlotte , Petit Angélique , Collin Anne , Houssier Marianne , Métayer-Coustard Sonia , Panserat Stéphane , Pitel Frédérique , Coustham Vincent
Published: January 2022

Early development is a critical period during which environmental influences can have a significant impact on the health, welfare, robustness and performance of livestock. In oviparous vertebrates, such as birds, embryonic development takes place entirely in the egg. This allows the effects of environmental cues to be studied directly on the developing embryo. Interestingly, beneficial effects have been identified in several studies, leading to innovative procedures to improve the phenotype of the animals in the long term. In this review, we discuss the effects of early temperature and dietary programming strategies that both show promising results, as well as their potential transgenerational effects. The timing, duration and intensity of these procedures are critical to ensure that they produce beneficial effects without affecting animal survival or final product quality. For example, cyclic increases in egg incubation temperature have been shown to improve temperature tolerance and promote muscular growth in chickens or fatty liver production in mule ducks. In ovo feeding has also been successfully used to enhance digestive tract maturation, optimize chick development and growth, and thus obtain higher quality chicks. In addition, changes in the nutritional availability of methyl donors, for example, was shown to influence offspring phenotype. The molecular mechanisms behind early phenotype programming are still under investigation and are probably epigenetic in nature as shown by recent work in chickens.
The GDF11 Promotes Nerve Regeneration After Sciatic Nerve Injury in Adult Rats by Promoting Axon Growth and Inhibiting Neuronal Apoptosis
Authors: Lin Junhao , Shi Jie , Min Xiang , Chen Si , Zhao Yunpeng , Zhang Yuanqiang , Cheng Lei
Published: January 2022

Introduction: Sciatic nerve injury is a common injury of the nervous system. Stem cell-based therapies, drug-based therapies and rehabilitation physiotherapy therapies are currently available, but their limited therapeutic efficacy limits their use. Here, we aimed to explore a novel lentiviral-based gene therapeutic strategy and to elaborate its mechanism.

Materials and Methods: Recombinant GDF11 protein was used for the in vitro treatment of dorsal root ganglion (DRG) cells. Lentivirus was used to construct a vector system for the in vivo expression of GDF11. The nerve conduction function was detected using action-evoked potentials at different time periods, and the regulatory effect of nerves on target organs was detected by weighing the gastrocnemius muscle. Immunofluorescence of NF200 and S100 was used to show the regeneration of the sciatic nerve, and myelin and Nissl staining were performed to observe the pathological features of the tissue. Western was used to validate signaling pathways. The expression of related genes was observed by qPCR and Western blotting, and cell apoptosis was detected by flow cytometry.

Result: GDF11 promotes the axonal growth of DRG cells and inhibits DGR cell apoptosis in vitro. GDF11 acts by activating the Smad pathway. GDF11 promotes the recovery of damaged sciatic nerve function in rats, the regeneration of damaged sciatic nerves in rats, and myelin regeneration of damaged sciatic nerves in rats. GDF11 also exerts a protective effect on neuronal cells in rats.

Conclusion: Based on the present study, we conclude that GDF11 promotes axonal growth and inhibits DRG cell apoptosis in vitro through the Smad pathway, and lentivirus-mediated GDF11 overexpression in vivo can promote the recovery of sciatic nerves after transection by promoting axonal growth and inhibiting neuronal apoptosis in the spinal cord.
The Wnt Signaling Pathway in Diabetic Nephropathy
Authors: Wang Haiying , Zhang Ran , Wu Xinjie , Chen Yafen , Ji Wei , Wang Jingsuo , Zhang Yawen , Xia Yong , Tang Yiqun , Yuan Jinxiang
Published: January 2022

Diabetic nephropathy (DN) is a serious kidney-related complication of both type 1 and type 2 diabetes mellitus (T1DM, T2DM) and the second major cause of end-stage kidney disease. DN can lead to hypertension, edema, and proteinuria. In some cases, DN can even progress to kidney failure, a life-threatening condition. The precise etiology and pathogenesis of DN remain unknown, although multiple factors are believed to be involved. The main pathological manifestations of DN include mesangial expansion, thickening of the glomerular basement membrane, and podocyte injury. Eventually, these pathological manifestations will lead to glomerulosclerosis, thus affecting renal function. There is an urgent need to develop new strategies for the prevention and treatment of DN. Existing evidence shows that the Wnt signaling cascade plays a key role in regulating the development of DN. Previous studies focused on the role of the Wnt canonical signaling pathway in DN. Subsequently, accumulated evidence on the mechanism of the Wnt non-canonical signaling indicated that Wnt/Ca2+ and Wnt/PCP also have essential roles in the progression of DN. In this review, we summarize the specific mechanisms of Wnt signaling in the occurrence and development of DN in podocyte injury, mesangial cell injury, and renal fibrosis. Also, to elucidate the significance of the Wnt canonical pathway in the process of DN, we uncovered evidence supporting that both Wnt/PCP and Wnt/Ca2+ signaling are critical for DN development.
RNA Secondary Structurome Revealed Distinct Thermoregulation in Plasmodium falciparum
Authors: Qi Yanwei , Zhang Yuhong , Mu Quankai , Zheng Guixing , Zhang Mengxin , Chen Bingxia , Huang Jun , Ma Changling , Wang Xinhua
Published: January 2022

The development of Plasmodium parasites, a causative agent of malaria, requests two hosts and the completion of 11 different parasite stages during development. Therefore, an efficient and fast response of parasites to various complex environmental changes, such as ambient temperature, pH, ions, and nutrients, is essential for parasite development and survival. Among many of these environmental changes, temperature is a decisive factor for parasite development and pathogenesis, including the thermoregulation of rRNA expression, gametogenesis, and parasite sequestration in cerebral malaria. However, the exact mechanism of how Plasmodium parasites rapidly respond and adapt to temperature change remains elusive. As a fundamental and pervasive regulator of gene expression, RNA structure can be a specific mechanism for fine tuning various biological processes. For example, dynamic and temperature-dependent changes in RNA secondary structures can control the expression of different gene programs, as shown by RNA thermometers. In this study, we applied the in vitro and in vivo transcriptomic-wide secondary structurome approach icSHAPE to measure parasite RNA structure changes with temperature alteration at single-nucleotide resolution for ring and trophozoite stage parasites. Among 3,000 probed structures at different temperatures, our data showed structural changes in the global transcriptome, such as S-type rRNA, HRPII gene, and the erythrocyte membrane protein family. When the temperature drops from 37°C to 26°C, most of the genes in the trophozoite stage cause significantly more changes to the RNA structure than the genes in the ring stage. A multi-omics analysis of transcriptome data from RNA-seq and RNA structure data from icSHAPE reveals that the specific RNA secondary structure plays a significant role in the regulation of transcript expression for parasites in response to temperature changes. In addition, we identified several RNA thermometers (RNATs) that responded quickly to temperature changes. The possible thermo-responsive RNAs in Plasmodium falciparum were further mapped. To this end, we identified dynamic and temperature-dependent RNA structural changes in the P. falciparum transcriptome and performed a comprehensive characterization of RNA secondary structures over the course of temperature stress in blood stage development. These findings not only contribute to a better understanding of the function of the RNA secondary structure but may also provide novel targets for efficient vaccines or drugs.
Isothiocyanate From Moringa oleifera Seeds Inhibits the Growth and Migration of Renal Cancer Cells by Regulating the PTP1B-dependent Src/Ras/Raf/ERK Signaling Pathway
Authors: Xie Jing , Qian Ying-Yan , Yang Yang , Peng Lin-Jie , Mao Jia-Ying , Yang Ming-Rong , Tian Yang , Sheng Jun
Published: January 2022

Moringa oleifera Lam. is a tropical and subtropical plant that has been used for centuries as both food and traditional medicine. 4-[(α-L-Rhamnosyloxy) benzyl] isothiocyanate (MIC-1) is an active substance in M. oleifera, with anti-cancer activity. However, whether MIC-1 exerts anti-renal cancer effects is unknown. Therefore, the aim of the present study was to evaluate the effects of MIC-1 on the growth and migration of renal cell carcinoma (RCC) cells and to identify the putative underlying mechanism. We found that, among 30 types of cancer cells, MIC-1 exerted the strongest growth inhibitory effects against 786-O RCC cells. In addition, MIC-1 (10 μM) significantly inhibited the growth of five RCC cell lines, including 786-O, OSRC-2, 769-P, SK-NEP-1, and ACHN cells, but was not toxic to normal renal (HK2) cells. Also, MIC-1 suppressed 786-O and 769-P cell migration and invasion abilities, and reduced the expression of matrix metalloproteinase (MMP)-2 and MMP-9. Furthermore, MIC-1 induced apoptosis and cell cycle arrest, increased Bax/Bcl-2 ratio, and decreased cell cycle-related protein expression in 786-O cells and 769-P cells. Molecular docking and small-molecule interaction analyses with PTP1B both showed that MIC-1 inhibited PTP1B activity by binding to its active site through hydrogen bonding and hydrophobic interactions. Additionally, MIC-1 could suppress the growth and migration of 786-O cells by inhibiting PTP1B-mediated activation of the Src/Ras/Raf/ERK signaling pathway. In vivo experiments further showed that MIC-1 markedly inhibited the growth of xenograft tumors in mice, and greatly increased Bax/Bcl-2 ratio in tumor tissues. In addition, MIC-1 had no effect on the PTP1B-dependent Src/Ras/Raf/ERK signaling pathway in HCT-116 cells, Hep-G2 cells, and A431 cells. Overall, our data showed that MIC-1 could be a promising, non-toxic, natural dietary supplement for the prevention and treatment of renal cancer.
Scenes From Tick Physiology: Proteins of Sialome Talk About Their Biological Processes
Authors: Fernández-Ruiz Natalia , Estrada-Peña Agustín
Published: January 2022

Ticks are blood-sucking parasites with different strategies of feeding depending on the tick family. The major families are Ixodidae or Argasidae, being slow or fast feeders, respectively. In the recent years, the advances in molecular sequencing techniques have enabled to gain knowledge about the proteome of the tick’s salivary glands. But an holistic view of the biological processes underlying the expression of the sialome has been neglected. In this study we propose the use of standard biological processes as a tool to draw the physiology of the tick’s salivary glands. We used published data on the sialome of Rhipicephalus sanguineus s.l. (Ixodidae) and Ornithodoros rostratus (Argasidae). A partial set of proteins obtained by these studies were used to define the biological process(es) in which proteins are involved. We used a directed network construction in which the nodes are proteins (source) and biological processes (target), separately for the low-level processes (“children”) and the top-level ones (“parents”). We applied the method to feeding R. sanguineus at different time slices, and to different organs of O. rostratus. The network connects the proteins and the processes with a strength directly proportional to the transcript per millions of each protein. We used PageRank as a measure of the importance of each biological process. As suggested in previous studies, the sialome of unfed R. sanguineus express about 30% less biological processes than feeding ticks. Another decrease (25%) is noticed at the middle of the feeding and before detachment. However, top-level processes are deeply affected only at the onset of feeding, demonstrating a redundancy in the feeding. When ixodid-argasid are compared, large differences were observed: they do not share 91% of proteins, but share 90% of the biological processes. However, caution must be observed when examining these results. The hypothesis of different proteins linked to similar biological process(es) in both ticks is an extreme not confirmed in this study. Considering the limitations of this study, carried out with a selected set of proteins, we propose the networks of proteins of sialome linked to their biological processes as a tool aimed to explain the biological processes behind families of proteins.
Establishment of a Risk Score Model for Early Prediction of Severe H1N1 Influenza
Authors: Lin Siran , Peng YuBing , Xu Yuzhen , Zhang Wei , Wu Jing , Zhang Wenhong , Shao Lingyun , Gao Yan
Published: January 2022

H1N1 is the most common subtype of influenza virus circulating worldwide and can cause severe disease in some populations. Early prediction and intervention for patients who develop severe influenza will greatly reduce their mortality. In this study, we conducted a comprehensive analysis of 180 PBMC samples from three published datasets from the GEO DataSets. Differentially expressed gene (DEG) analysis and weighted correlation network analysis (WGCNA) were performed to provide candidate DEGs for model building. Functional enrichment and CIBERSORT analyses were also performed to evaluate the differences in composition and function of PBMCs between patients with severe and mild disease. Finally, a risk score model was built using lasso regression analysis, with six genes (CX3CR1, KLRD1, MMP8, PRTN3, RETN and SCD) involved. The model performed moderately in the early identification of patients that develop severe H1N1 disease.
Targeting Epigenetic Mechanisms in Vascular Aging
Authors: Lin Zhongxiao , Ding Qian , Li Xinzhi , Feng Yuliang , He Hao , Huang Chuoji , Zhu YiZhun
Published: January 2022

Environment, diseases, lack of exercise, and aged tendency of population have becoming crucial factors that induce vascular aging. Vascular aging is unmodifiable risk factor for diseases like diabetes, hypertension, atherosclerosis, and hyperlipidemia. Effective interventions to combat this vascular function decline is becoming increasingly urgent as the rising hospitalization rate caused by vascular aging-related diseases. Fortunately, recent transformative omics approaches have enabled us to examine vascular aging mechanisms at unprecedented levels and precision, which make our understanding of slowing down or reversing vascular aging become possible. Epigenetic viz. DNA methylation, histone modifications, and non-coding RNA-based mechanisms, is a hallmark of vascular aging, its deregulation leads to aberrant transcription changes in tissues. Epigenetics mechanisms by mediating covalent modifications to DNA and histone proteins, consequently, influence the sensitivity and activities of signaling pathways in cells and tissues. A growing body of evidence supports correlations between epigenetic changes and vascular aging. In this article, we will provide a comprehensive overview of epigenetic changes associated with vascular aging based on the recent findings with a focus on molecular mechanisms of action, strategies to reverse epigenetic changes, and future perspectives.
The Diagnostic Accuracy of One-Step Nucleic Acid Amplification for Lymph Node Metastases of Papillary Thyroid Carcinoma： A Systematic Review and Meta-Analysis
Authors: Wang Xiaofei , Zheng Xun , Zhu Jingqiang , Li Zhihui , Wei Tao
Published: January 2022

Background

One-step nucleic acid amplification (OSNA) analysis is a molecular diagnostic technique for lymph node metastases (LNMs) by quantifying cytokeratin 19(CK 19) mRNA. We aim to evaluate the intraoperative diagnostic accuracy of OSNA assay for LNMs of papillary thyroid carcinoma (PTC).

Methods

PubMed, Embase, Cochrane Library, and Web of Science databases were searched to retrieve related literature. A meta-analysis was performed using STATA11.0, Meta-Disc 1.4 and RevMan 5.3.

Results

This meta-analysis included six studies involving 987 lymph nodes from 194 patients. The pooled sensitivity, specificity, and area under the summary receiver-operating characteristic curve (AUC) of OSNA for detecting LNM were 0.88, 0.90, and 0.95, respectively.

Conclusion

OSNA assay is an accurate molecular diagnosis for intraoperative detection of lymph node metastasis in PTC.
Metabolic Syndrome and Its Components Are Associated With Altered Amino Acid Profile in Chinese Han Population
Authors: Sun Shuiya , He Dongjuan , Luo Cheng , Lin Xihua , Wu Jiahua , Yin Xueyao , Jia Chengfang , Pan Qianqian , Dong Xuehong , Zheng Fenping , Li Hong , Zhou Jiaqiang
Published: January 2022

Objective

Recent studies have found that the levels of plasma amino acids, such as branched-chain amino acids and aromatic amino acids, were associated with visceral obesity, insulin resistance, future development of diabetes and cardiovascular diseases. However, few studies have involved a Chinese Han population. This study aimed to examine the association between amino acid profile and metabolic syndrome (MetS) and its components in the Chinese Han population.

Methods

This is a cross-sectional study, which enrolled a cohort of 473 participants from a community. We employed the isotope internal standard method to determine the plasma concentrations of 28 amino acids using high-performance liquid chromatography-tandem mass spectrometry (LC/MS). Participants were divided into MetS (n = 72) and non-MetS groups (n = 401) to analyze the association between amino acids and MetS and its components.

Results

The prevalence of MetS was 15.2% according to the criteria. Plasma concentrations of isoleucine (Ile), leucine (Leu), valine (Val), tyrosine (Tyr), tryptophan (Trp), phenylalanine (Phe), glutamic acid (Glu), aspartic acid (Asp), alanine (Ala), histidine (His), methionine (Met), asparagine (Asn), and proline (Pro) were significantly higher in the MetS group than those in the non-MetS group (P < 0.05), but taurine (Tau) was significantly lower (P < 0.05). When MetS components were increased, the concentrations of these 13 amino acids significantly increased (P < 0.05), but Tau concentration was significantly decreased (P < 0.05). We extracted the amino acid profile by principal component analysis (PCA), PC1 and PC2, which extracted from the 14 amino acids, were significantly associated with MetS (odds ratio, 95% confidence interval: 1.723, 1.325–2.085 and 1.325, 1.043–1.684, respectively). A total of 260 non-MetS participants were followed up effectively, and 42 participants developed new-onset MetS within 5 years. We found that the amino acid profile of PC1 was linked to the occurrence of future MetS. Decreased Tau was correlated with the future development of MetS.

Conclusion

Participants with MetS exhibit an abnormal amino acid profile, and its components gradually increase when these amino acids are altered. Amino acid PCA profile can be employed for assessing and monitoring MetS risk. Finally, decreased Tau may be linked to the future development of MetS.
Relationship Among Traditional Semen Parameters, Sperm DNA Fragmentation, and Unexplained Recurrent Miscarriage: A Systematic Review and Meta-Analysis
Authors: Dai Yanpeng , Liu Junjie , Yuan Enwu , Li Yushan , Shi Ying , Zhang Linlin
Published: January 2022

Several studies have explored the relationship among traditional semen parameters, sperm DNA fragmentation (SDF), and unexplained recurrent miscarriage (RM); however, the findings remain controversial. Hence, we conducted a meta-analysis to explore the relationship among traditional semen parameters, SDF, and unexplained RM. Multiple databases, including PubMed, Google Scholar, MEDLINE, Embase, Cochrane Library, Web of Science, and China National Knowledge Infrastructure (CNKI), were searched to identify relevant publications. From the eligible publications, data were extracted independently by two researchers. A total of 280 publications were identified using the search strategy. According to the inclusion/exclusion criteria, 19 publications were eligible. A total of 1182 couples with unexplained RM and 1231 couples without RM were included in this meta-analysis to assess the relationship among traditional semen parameters, SDF, and unexplained RM. Our results showed that couples with unexplained RM had significantly increased levels of SDF and significantly decreased levels of total motility and progressive motility compared with couples without RM, although significant differences were not observed in the semen volume, sperm concentration, and total sperm count between couples with and without RM. The SDF assay may be considered for inclusion in evaluations of couples with unexplained RM.
Habitation Sites Influence Tree Community Assemblages in the Great Bear Rainforest, British Columbia, Canada
Authors: Schang Kyle , Cox Kieran , Trant Andrew J.
Published: January 2022

Identifying how past human actions have influenced their environment is essential for understanding the ecological factors that structure contemporary ecosystems. Intertidal resource use by Indigenous Peoples for thousands of years has led to habitation sites containing vast shell midden deposits and facilitating long-term impacts on soil chemistry and drainage. Here we examine how these shell middens have impacted various forest metrics, such as species diversity, community composition, canopy height, and regeneration recruitment to determine if forests on habitation sites differ from the surrounding matrix. We surveyed known habitation sites with archeological evidence indicating past year-round human occupation, within the Hakai Lúxvbálís Conservancy on Calvert and Hecate Islands within the Great Bear Rainforest along British Columbia’s Central Coast. Our results demonstrate that habitation sites exhibit lower tree species richness, less relative species abundances, as such, displayed lower Shannon diversity and inverse Simpson values. The composition of tree communities on habitation sites was statistically different, with western hemlock and western redcedar densities increasing on non-habitation sites. Conversely, regeneration diversity at habitation sites was more even and exhibited elevated Shannon diversity and inverse Simpson values. The community composition of regeneration was more consistent among habitation and non-habitation sites; however, western redcedar, western hemlock and Sitka spruce were more abundant at habitation sites. For all tree species, maximum height was higher within the habitation sites; however, this trend was the most notable in western redcedar and Sitka spruce, which increased by an average of 4.8 m relative to non-habitation sites. Collectively, our findings suggest that long-term habitation alters forest community compositions. The landscape alterations within habitation sites promote conditions needed to support diverse, even, and abundant regeneration communities and consequently increase the height of the dominant coastal tree species. Thus, our results offer evidence that long-term influence by Indigenous communities have a persistent influence on coastal forests.