bluuebunny/crossref_metadata_2025 huggingface dataset:
Preclinical Evaluation of Long-Term Neuroprotective Effects of BDNF-Engineered Mesenchymal Stromal Cells as Intravitreal Therapy for Chronic Retinal Degeneration in Rd6 Mutant Mice
Authors: Lejkowska Renata , Kawa Miłosz Piotr , Pius-Sadowska Ewa , Rogińska Dorota , Łuczkowska Karolina , Machaliński Bogusław , Machalińska Anna
Published: February 2019

This study aimed to investigate whether the transplantation of genetically engineered bone marrow-derived mesenchymal stromal cells (MSCs) to overexpress brain-derived neurotrophic factor (BDNF) could rescue the chronic degenerative process of slow retinal degeneration in the rd6 (retinal degeneration 6) mouse model and sought to identify the potential underlying mechanisms. Rd6 mice were subjected to the intravitreal injection of lentivirally modified MSC-BDNF or unmodified MSC or saline. In vivo morphology, electrophysiological retinal function (ERG), and the expression of apoptosis-related genes, as well as BDNF and its receptor (TrkB), were assessed in retinas collected at 28 days and three months after transplantation. We observed that cells survived for at least three months after transplantation. MSC-BDNF preferentially integrated into the outer retinal layers and considerably rescued damaged retinal cells, as evaluated by ERG and immunofluorescence staining. Additionally, compared with controls, the therapy with MSC-BDNF was associated with the induction of molecular changes related to anti-apoptotic signaling. In conclusion, BDNF overexpression observed in retinas after MSC-BDNF treatment could enhance the neuroprotective properties of transplanted autologous MSCs alone in the chronically degenerated retina. This research provides evidence for the long-term efficacy of genetically-modified MSC and may represent a strategy for treating various forms of degenerative retinopathies in the future.
Molecular Research in Chronic Thromboembolic Pulmonary Hypertension
Authors: Opitz Isabelle , Kirschner Michaela B.
Published: February 2019

Chronic Thromboembolic Pulmonary Hypertension (CTEPH) is a debilitating disease, for which the underlying pathophysiological mechanisms have yet to be fully elucidated. Occurrence of a pulmonary embolism (PE) is a major risk factor for the development of CTEPH, with non-resolution of the thrombus being considered the main cause of CTEPH. Polymorphisms in the α-chain of fibrinogen have been linked to resistance to fibrinolysis in CTEPH patients, and could be responsible for development and disease progression. However, it is likely that additional genetic predisposition, as well as genetic and molecular alterations occurring as a consequence of tissue remodeling in the pulmonary arteries following a persistent PE, also play an important role in CTEPH. This review summarises the current knowledge regarding genetic differences between CTEPH patients and controls (with or without pulmonary hypertension). Mutations in BMPR2, differential gene and microRNA expression, and the transcription factor FoxO1 have been suggested to be involved in the processes underlying the development of CTEPH. While these studies provide the first indications regarding important dysregulated pathways in CTEPH (e.g., TGF-β and PI3K signaling), additional in-depth investigations are required to fully understand the complex processes leading to CTEPH.
A Control-Theoretic Model of Atherosclerosis
Authors: Formanowicz Dorota , Krawczyk Jacek B. , Perek Bartłomiej , Formanowicz Piotr
Published: February 2019

We propose a control-theoretic aggregate model of the progression of atherosclerosis plaque, a chronic inflammatory disease of the arterial wall, to study the basic features of this disease. In the model, we exploit the role of inflammation in the disease progression, and use statins—drugs commonly recommended in atherosclerosis—to control this progression. We use a logistic function to allow for constrained growth of plaque. In the model, both the patient’s age and overall health impact the plaque growth and its sensitivity to statins. The model parameters are estimated using original data, or calibrated using published research as well as our own clinical and laboratory studies. We contend that our model helps to gauge the statins’ impact on a patient’s plaque thickness, hence the disease’s progression and cardiovascular risk, without requiring artery scans.
Clonal Hematopoiesis with Oncogenic Potential (CHOP): Separation from CHIP and Roads to AML
Authors: Valent Peter , Kern Wolfgang , Hoermann Gregor , Milosevic Feenstra Jelena D. , Sotlar Karl , Pfeilstöcker Michael , Germing Ulrich , Sperr Wolfgang R. , Reiter Andreas , Wolf Dominik , Arock Michel , Haferlach Torsten , Horny Hans-Peter
Published: February 2019

The development of leukemia is a step-wise process that is associated with molecular diversification and clonal selection of neoplastic stem cells. Depending on the number and combinations of lesions, one or more sub-clones expand/s after a variable latency period. Initial stages may develop early in life or later in adulthood and include premalignant (indolent) stages and the malignant phase, defined by an acute leukemia. We recently proposed a cancer model in which the earliest somatic lesions are often age-related early mutations detectable in apparently healthy individuals and where additional oncogenic mutations will lead to the development of an overt neoplasm that is usually a preleukemic condition such as a myelodysplastic syndrome. These neoplasms may or may not transform to overt acute leukemia over time. Thus, depending on the type and number of somatic mutations, clonal hematopoiesis (CH) can be divided into CH with indeterminate potential (CHIP) and CH with oncogenic potential (CHOP). Whereas CHIP mutations per se usually create the molecular background of a neoplastic process, CHOP mutations are disease-related or even disease-specific lesions that trigger differentiation and/or proliferation of neoplastic cells. Over time, the acquisition of additional oncogenic events converts preleukemic neoplasms into secondary acute myeloid leukemia (sAML). In the present article, recent developments in the field are discussed with a focus on CHOP mutations that lead to distinct myeloid neoplasms, their role in disease evolution, and the impact of additional lesions that can drive a preleukemic neoplasm into sAML.
Endogenous Retroviruses Function as Gene Expression Regulatory Elements During Mammalian Pre-implantation Embryo Development
Authors: Fu Bo , Ma Hong , Liu Di
Published: February 2019

Pre-implantation embryo development encompasses several key developmental events, especially the activation of zygotic genome activation (ZGA)-related genes. Endogenous retroviruses (ERVs), which are regarded as “deleterious genomic parasites”, were previously considered to be “junk DNA”. However, it is now known that ERVs, with limited conservatism across species, mediate conserved developmental processes (e.g., ZGA). Transcriptional activation of ERVs occurs during the transition from maternal control to zygotic genome control, signifying ZGA. ERVs are versatile participants in rewiring gene expression networks during epigenetic reprogramming. Particularly, a subtle balance exists between ERV activation and ERV repression in host–virus interplay, which leads to stage-specific ERV expression during pre-implantation embryo development. A large portion of somatic cell nuclear transfer (SCNT) embryos display developmental arrest and ZGA failure during pre-implantation embryo development. Furthermore, because of the close relationship between ERV activation and ZGA, exploring the regulatory mechanism underlying ERV activation may also shed more light on the enigma of SCNT embryo development in model animals.
Response of Tibetan Wild Barley Genotypes to Drought Stress and Identification of Quantitative Trait Loci by Genome-Wide Association Analysis
Authors: Zhang Mian , Fu Man-Man , Qiu Cheng-Wei , Cao Fangbin , Chen Zhong-Hua , Zhang Guoping , Wu Feibo
Published: February 2019

Tibetan wild barley has been identified to show large genetic variation and stress tolerance. A genome-wide association (GWA) analysis was performed to detect quantitative trait loci (QTLs) for drought tolerance using 777 Diversity Array Technology (DArT) markers and morphological and physiological traits of 166 Tibetan wild barley accessions in both hydroponic and pot experiments. Large genotypic variation for these traits was found; and population structure and kinship analysis identified three subpopulations among these barley genotypes. The average LD (linkage disequilibrium) decay distance was 5.16 cM, with the minimum on 6H (0.03 cM) and the maximum on 4H (23.48 cM). A total of 91 DArT markers were identified to be associated with drought tolerance-related traits, with 33, 26, 16, 1, 3, and 12 associations for morphological traits, H+K+-ATPase activity, antioxidant enzyme activities, malondialdehyde (MDA) content, soluble protein content, and potassium concentration, respectively. Furthermore, 7 and 24 putative candidate genes were identified based on the reference Meta-QTL map and by searching the Barleymap. The present study implicated that Tibetan annual wild barley from Qinghai–Tibet Plateau is rich in genetic variation for drought stress. The QTLs detected by genome-wide association analysis could be used in marker-assisting breeding for drought-tolerant barley genotypes and provide useful information for discovery and functional analysis of key genes in the future.
Diversity of Termite Breeding Systems
Authors: Vargo Edward L.
Published: February 2019

Termites are social insects that live in colonies headed by reproductive castes. The breeding system is defined by the number of reproductive individuals in a colony and the castes to which they belong. There is tremendous variation in the breeding system of termites both within and among species. The current state of our understanding of termite breeding systems is reviewed. Most termite colonies are founded by a primary (alate-derived) king and queen who mate and produce the other colony members. In some species, colonies continue throughout their life span as simple families headed by the original king and queen. In others, the primary king and queen are replaced by numerous neotenic (nymph- or worker-derived) reproductives, or less commonly primary reproductives, that are descendants of the original founding pair leading to inbreeding in the colony. In still others, colonies can have multiple unrelated reproductives due to either founding the colonies as groups or through colony fusion. More recently, parthenogenetic reproduction has shown to be important in some termite species and may be widespread. A major challenge in termite biology is to understand the ecological and evolutionary factors driving the variation in termite breeding systems.
Sickly Sweet: Insecticidal Polyols Induce Lethal Regurgitation in Dipteran Pests
Authors: Díaz-Fleischer Francisco , Arredondo José , Lasa Rodrigo , Bonilla Carlos , Debernardi Diana , Pérez-Staples Diana , Williams Trevor
Published: February 2019

Polyols are commonly used in food and medicines as sweeteners and preservatives but may also have insecticidal properties against some species of Diptera. Here we compared the insecticidal activity and feeding response of glycerol and propylene glycol (PG) on two tephritids: Anastrepha ludens and Anastrepha obliqua, and the drosophilid Drosophila suzukii. First, flies were exposed to solutions of 50% sucrose and the two polyols at concentrations of 1.67 M, 2.78 M and 4.18 M for 24 h and then observed at 24 h intervals for a period of three days. Both polyols elicited strong regurgitation behavior in the three flies and killed them. Regurgitation apparently also reduced flies’ body weight, and this was particularly apparent in insects that fed on 4.18 M PG solutions. A high percentage of individuals exposed to PG solutions perished after 72 h. The number of proboscis extensions, which is associated with feeding preference, was lower in the 4.18 M polyols + sucrose mixtures than in the 50% sucrose solution. Glycerol had a lower insecticidal effect in Anastrepha spp. and very little insecticidal effect in D. suzukii. Finally, elevated regurgitation and mortality was confirmed in A. ludens treated with 1.0–2.78 M of erythritol plus sucrose. Our results demonstrate that PG, and to a lower extent glycerol, have the potential for being used as a safer method of insect pest control. The hyper-regurgitation response may contribute to the insecticidal properties of these polyols in Diptera.
NT-ProBNP Predicts Total Mortality, Emergency Department Visits, Hospitalization, Intensive-Care Unit Admission, and Cardiovascular Events in Hemodialysis Patients
Authors: Chen Yi-Hsin , Fu Yun-Ching , Wu Ming-Ju
Published: February 2019

N-terminal pro b-type natriuretic peptide (NT-proBNP) was considered a prognostic factor for mortality in hemodialysis patients in previous studies. However, NT-proBNP has not been fully explored in terms of predicting other clinical outcomes in hemodialysis patients. This study aimed to investigate if NT-proBNP could predict emergency department (ED) visits, hospitalization, admission to intensive-care unit (ICU), and cardiovascular incidents in hemodialysis patients. Serum NT-proBNP and other indicators were collected in 232 hemodialysis patients. Patients were followed up for three years or until mortality. Outcomes included mortality, number of ED visits, hospitalizations, admissions to ICU, and cardiovascular events. NT-proBNP was found to predict recurrent ER visits, hospitalization, admission to ICU, cardiovascular events, and mortality, after adjusting for covariates. Time-dependent area under the curve (AUC) was used to evaluate the NT-proBNP predicting ability. Using time-dependent AUC, NT-proBNP has good predictive ability for mortality, ED visit, hospitalization, ICU admission, and cardiovascular events with the best predictive ability occurring at approximately 1 year, and 5th, 62nd, 63rd, and 63rd days respectively. AUC values for predicting mortality, hospitalization, and ICU admission decreased significantly after one year. NT-proBNP can be applied in predicting ED visits but is only suitable for the short-term. NT-proBNP may be used for predicting mortality in the long term.
Troponin Testing for Assessing Sepsis-Induced Myocardial Dysfunction in Patients with Septic Shock
Authors: Kim June-Sung , Kim Muyeol , Kim Youn-Jung , Ryoo Seung Mok , Sohn Chang Hwan , Ahn Shin , Kim Won Young
Published: February 2019

(1) Background: Myocardial dysfunction in patients with sepsis is not an uncommon phenomenon, yet reported results are conflicting and there is no objective definition. Measurement of troponin may reflect the state of the heart and may correlate with echocardiographically derived data. This study aimed to evaluate the role of admission and peak troponin-I testing for the identification of sepsis-induced myocardial dysfunction (SIMD) by transthoracic echocardiography (TTE). (2) Methods: This was a retrospective cohort study using a prospective registry of septic shock at an Emergency Department from January 2011 and April 2017. All 1,776 consecutive adult septic shock patients treated with protocol-driven resuscitation bundle therapy and tested troponin-I were enrolled. SIMD was defined as left ventricular (LV) systolic/diastolic dysfunction, right ventricular (RV) diastolic dysfunction, or global/regional wall motion abnormalities (WMA). (3) Results: Of 660 (38.4%) septic shock patients with an elevated hs-TnI (≥0.04 ng/mL) at admission, 397 patients underwent TTE and 258 cases (65%) showed SIMD (LV systolic dysfunction (n = 163, 63.2%), LV diastolic dysfunction (n = 104, 40.3%), RV dysfunction (n = 97, 37.6%), and WMA (n = 186, 72.1%)). In multivariate analysis, peak hs-TnI (odds ratio 1.03, 95% confidence interval 1.01–1.06, p = 0.008) and ST-T wave changes in the electrocardiogram (odds ratio 1.82, 95% confidence interval 1.04–2.39, p = 0.013) were associated with SIMD, in contrast to hs-TnI level at admission. The area under the curve of peak hs-TnI was 0.668. When the peak hs-TnI cutoff value was 0.634 ng/mL, the sensitivity and specificity for SIMD were 58.6% and 59.1%, respectively. 4) Conclusions: About two-thirds of patients with an elevated hs-TnI level have various cardiac dysfunctions in terms of TTE. Rather than the initial level, the peak hs-TnI and ST-T change may be considered as a risk factor of SIMD.
Carbon Fiber Reinforced PEEK Composites Based on 3D-Printing Technology for Orthopedic and Dental Applications
Authors: Han Xingting , Yang Dong , Yang Chuncheng , Spintzyk Sebastian , Scheideler Lutz , Li Ping , Li Dichen , Geis-Gerstorfer Jürgen , Rupp Frank
Published: February 2019

Fused deposition modeling (FDM) is a rapidly growing three-dimensional (3D) printing technology and has great potential in medicine. Polyether-ether-ketone (PEEK) is a biocompatible high-performance polymer, which is suitable to be used as an orthopedic/dental implant material. However, the mechanical properties and biocompatibility of FDM-printed PEEK and its composites are still not clear. In this study, FDM-printed pure PEEK and carbon fiber reinforced PEEK (CFR-PEEK) composite were successfully fabricated by FDM and characterized by mechanical tests. Moreover, the sample surfaces were modified with polishing and sandblasting methods to analyze the influence of surface roughness and topography on general biocompatibility (cytotoxicity) and cell adhesion. The results indicated that the printed CFR-PEEK samples had significantly higher general mechanical strengths than the printed pure PEEK (even though there was no statistical difference in compressive strength). Both PEEK and CFR-PEEK materials showed good biocompatibility with and without surface modification. Cell densities on the “as-printed” PEEK and the CFR-PEEK sample surfaces were significantly higher than on the corresponding polished and sandblasted samples. Therefore, the FDM-printed CFR-PEEK composite with proper mechanical strengths has potential as a biomaterial for bone grafting and tissue engineering applications.
Automatic Shoreline Position and Intertidal Foreshore Slope Detection from X-Band Radar Images Using Modified Temporal Waterline Method with Corrected Wave Run-up
Authors: Kumar Dipankar , Takewaka Satoshi
Published: February 2019

Automatic and accurate shoreline position and intertidal foreshore slope detection are challenging and significantly important for coastal dynamics. In the present study, a time series shoreline position and intertidal foreshore slope have been automatically detected using modified Temporal Waterline Method (mTWM) from time-averaged X-band radar images captured throughout the course of two-week tidal cycle variation over an area spanning 5.6 km on the Hasaki coast between 12 April 2005 and 31 December 2008. The methodology is based on the correlation map between the pixel intensity variation of the time-averaged X-band radar images and the binary signal of the tide level ranging from −0.8 m to 0.8 m. In order to ensure the binary signal represented each of the water levels in the intertidal shore profile, determining the water level direction-wise bottom elevation is considered as the modification. Random gaps were detected in the captured images owing to the unclear or oversaturation of the waterline signal. A horizontal shift in the detected shoreline positions was observed compared to the survey data previously collected at Hasaki Oceanographical Research Station (HORS). This horizontal shift can be attributed to wave breaking and high wave conditions. Wave set-up and run-up are the effects of wave breaking and high wave conditions, respectively. The correction of the wave set-up and run-up is considered to allow the upward shift of the water level position, as well as shoreline position, to the landward direction. The findings indicate that the shoreline positions derived by mTWM with the corrected wave run-up reasonably agree with the survey data. The mean absolute bias (MAB) between the survey data and the shoreline positions detected using mTWM with the corrected wave run-up is approximately 5.9 m, which is theoretically smaller than the spatial resolution of the radar measurements. The random gaps in the mTWM-derived shoreline positions are filled by Garcia’s data filling algorithm which is a Penalized Least Squares regression method by means of the Discrete Cosine Transform (PLS-DCT). The MAB between survey data and the gap filled shoreline positions detected using TWM with corrected wave run-up is approximately 5.9 m. The obtained results indicate the accuracy of the mTWM with corrected wave run-up integrated with Garcia’s method compared to the survey observations. The executed approach in this study is considered as an efficient and robust tool to automatically detect shoreline positions and intertidal foreshore slopes extracted from X-band radar images with the consideration of wave run-up correction.
The Anisotropic Distortional Yield Surface Constitutive Model Based on the Chaboche Cyclic Plastic Model
Authors: Chen Jianyun , Zhang Keshi , Kuang Zheng , Hu Guijuan , Song Qiao , Chang Yanjun
Published: February 2019

Considering the cross effect in the evolution of subsequent yield surfaces for metals, an anisotropic distortional yield surface constitutive model is developed. By introducing an anisotropic distortional hardening function into the isotropic hardening part of the classical Chaboche rate-dependent constitutive model, the plastic-deformation-induced distortional and anisotropic hardening behaviors of subsequent yield surfaces are characterized. The experimental data of distortional yield surfaces for T2 pure copper under three different loading paths, including pre-tension, pre-torsion, and pre-tension-torsion proportional loading of 45-degree, are simulated by implementing the models into a numerical user defined material (UMAT) procedure based on the ABAQUS finite element package. To validate the anisotropic plastic model, the simulated yield surfaces are compared with experimental observations and predicted results for a crystal plasticity model and good agreement are noted. The simulations demonstrate that the proposed model can accurately capture the characteristics of the distortional yield surface and the anisotropic hardening process of the yield surface. Moreover, the distortional shapes of experimental subsequent yield surfaces in loading direction and opposite direction can be better revealed by the anisotropic plastic constitutive model than the crystal plastic constitutive model.
Microstructural Tuning of a Laser-Cladding Layer by Means of a Mix of Commercial Inconel 625 and AISI H13 Powders
Authors: Muro Maider , Leunda Josu , Artola Garikoitz , Soriano Carlos
Published: February 2019

The aim of this work is to evaluate the microstructural evolutions developed by mixing a corrosion-resistant and high-performance material with a high-hardness material in a coating obtained by laser-cladding technology. In this paper, five different mixtures of Inconel 625 alloy and AISI H13 steel powders have been deposited on a plate of 42CrMo4 steel using a 2.2 kW diode pumped Nd:YAG laser. The effect of adding tool steel to a Ni-based superalloy has been analyzed by the characterization of each cladded sample using optical microscopy and scanning electron microscopy (SEM). The precipitates observed in the samples have been analyzed by energy dispersive X-ray spectroscopy (EDS X-ray). SEM micrographs and EDS analysis indicate the existence of Laves phase. It has been observed that the presence of these precipitates is stabilized in a certain range of AISI H13 addition.
Effect of YAl2 Particles on the Corrosion Behavior of Mg–Li Matrix Composite in NaCl Solution
Authors: Chen Zihan , Bao Chonggao , Wu Guoqing , Jian Yongxin , Zhang Li
Published: February 2019

The strength of Mg–Li alloy is greatly improved by the composite strengthening of intermetallic compound YAl2 particles, but the low corrosion resistance of Mg–Li alloy is still the main factor that restricts the application of the alloy and its composites. In this paper, the effect of YAl2 particles on the corrosion behavior of Mg–Li alloy was systematically investigated. The results showed that the corrosion resistance of YAl2p/LA143 composite could be significantly improved, accounting for the formation of a transitional interface layer by adding YAl2 particles. The diffusion of yttrium and aluminum atoms from YAl2 particulates improved the stability of the surface film and enhanced the adhesion between the corrosion products and the substrate, which hindered further expansion of pitting.
Optimal Derivative-Free Root Finding Methods Based on Inverse Interpolation
Authors: Junjua Moin-ud-Din , Zafar Fiza , Yasmin Nusrat
Published: February 2019

Finding a simple root for a nonlinear equation f ( x ) = 0 , f : I ⊆ R → R has always been of much interest due to its wide applications in many fields of science and engineering. Newton’s method is usually applied to solve this kind of problems. In this paper, for such problems, we present a family of optimal derivative-free root finding methods of arbitrary high order based on inverse interpolation and modify it by using a transformation of first order derivative. Convergence analysis of the modified methods confirms that the optimal order of convergence is preserved according to the Kung-Traub conjecture. To examine the effectiveness and significance of the newly developed methods numerically, several nonlinear equations including the van der Waals equation are tested.
Lung Ultrasound B-lines Occurrence in Relation to Left Ventricular Function and Hydration Status in Hemodialysis Patients
Authors: Pardała Agnieszka , Lupa Mariusz , Chudek Jerzy , Kolonko Aureliusz
Published: February 2019

Background and objective: Reliable assessment of the fluid status in hemodialysis (HD) patients is often difficult. A lung ultrasound with an assessment of the B-lines (“lung comets” (LCs)) number is a novel hydration status measure. However, the occurrence of left ventricular dysfunction may have a significant effect on pulmonary congestion and further modulate the LC number. The aim of this study was to analyze to what extent left ventricular dysfunction, pulmonary hypertension, and hypervolemia affect the occurrence of LC in a cohort of prevalent HD patients. Material and methods: This cross-sectional study included 108 assessments performed in 54 patients who attended thrice weekly outpatient HD. Each patient’s fluid status was evaluated twice, prior to HD sessions, using echocardiography, LC number assessment, measurement of inferior vena cava (IVC) diameters, and bioelectric impedance analysis (BIA). Patients were stratified into three subgroups according to their LC number. Results: There were 76 separate assessments with mild (<14), 16 with moderate (14–30), and 16 with severe (>30) LC occurrence. There was a negative correlation between the LC number and left ventricular ejection fraction (LVEF), and positive correlations between the LC number and mitral gradient, and the left and right atrium area and volume, but not with the BIA-derived relative fluid overload. Multivariate linear regression analysis revealed that the LC number was proportionally related to the mitral gradient (β = 0.407 (0.247–0.567), p < 0.001) and IVC max diameter (β = 0.219 (0.060–0.378), p < 0.01), and was inversely related to LVEF (β = −0.431 (−0.580 to −0.282), p < 0.001). Conclusions: The number of LCs appears to reflect both overhydration and left ventricular dysfunction in our HD patients cohort. Therefore, heart failure must be considered as an important factor limiting the usefulness of LCs number assessment in this population.
Measuring Ocular Aberrations Sequentially Using a Digital Micromirror Device
Authors: Carmichael Martins Alessandra , Vohnsen Brian
Published: February 2019

The Hartmann–Shack wavefront sensor is widely used to measure aberrations in both astronomy and ophthalmology. Yet, the dynamic range of the sensor is limited by cross-talk between adjacent lenslets. In this study, we explore ocular aberration measurements with a recently-proposed variant of the sensor that makes use of a digital micromirror device for sequential aperture scanning of the pupil, thereby avoiding the use of a lenslet array. We report on results with the sensor using two different detectors, a lateral position sensor and a charge-coupled device (CCD) scientific camera, and explore the pros and cons of both. Wavefront measurements of a highly aberrated artificial eye and of five real eyes, including a highly myopic subject, are demonstrated, and the role of pupil sampling density, CCD pixel binning, and scanning speed are explored. We find that the lateral position sensor is mostly suited for high-power applications, whereas the CCD camera with pixel binning performs consistently well both with the artificial eye and for real-eye measurements, and can outperform a commonly-used wavefront sensor with highly aberrated wavefronts.
Recovery of Li(Ni0.33Mn0.33Co0.33)O2 from Lithium-Ion Battery Cathodes: Aspects of Degradation
Authors: Sieber Tim , Ducke Jana , Rietig Anja , Langner Thomas , Acker Jörg
Published: February 2019

Nickel–manganese–cobalt oxides, with LiNi0.33Mn0.33Co0.33O2 (NMC) as the most prominent compound, are state-of-the-art cathode materials for lithium-ion batteries in electric vehicles. The growing market for electro mobility has led to a growing global demand for Li, Co, Ni, and Mn, making spent lithium-ion batteries a valuable secondary resource. Going forward, energy- and resource-inefficient pyrometallurgical and hydrometallurgical recycling strategies must be avoided. We presented an approach to recover NMC particles from spent lithium-ion battery cathodes while preserving their chemical and morphological properties, with a minimal use of chemicals. The key task was the separation of the cathode coating layer consisting of NMC, an organic binder, and carbon black, from the Al substrate foil. This can be performed in water under strong agitation to support the slow detachment process. However, the contact of the NMC cathode with water leads to a release of Li+ ions and a fast increase in the pH. Unwanted side reactions may occur as the Al substrate foil starts to dissolve and Al(OH)3 precipitates on the NMC. These side reactions are avoided using pH-adjusted solutions with sufficiently high buffer capacities to separate the coating layer from the Al substrate, without precipitations and without degradation of the NMC particles.
Ups and Downs of Water Photodecolorization by Nanocomposite Polymer Nanofibers
Authors: Homaeigohar Shahin , Botcha Niharika Krishna , Zarie Eman. S. , Elbahri Mady
Published: February 2019

Given the exponentially expanding water pollution causing water scarcity, there is an urgent need for operative nanotechnological systems that can purify water, with insignificant energy consumption, and rapidly. Here, we introduce a nanocomposite system based on TiO2 nanoparticles (NPs) and PES nanofibers (NFs) that can adsorb and then photodecompose organic water pollutants such as dye molecules. We evaluate pros and cons of this system with respect to its purification efficiency and structural properties that can be impacted by the photocatalytic activity of the nanofillers. While the material is superhydrophilic and able to remove 95% methylene blue (MB) from water via adsorption/photodecomposition, its thermomechanical properties decline upon UV irradiation. However, these properties still remain at the level of the neat NFs. The removal behavior is modeled by the first- and second-order kinetic models from the kinetic point of view. The nanocomposite NFs’ removal behavior complies much better with the second-order kinetic model. Overall, such feedbacks implied that the nanocomposite can be effectively applied for water treatment and the structural properties are still as reliable as those of the neat counterpart.
Maternal Choline Supplementation Modulates Placental Markers of Inflammation, Angiogenesis, and Apoptosis in a Mouse Model of Placental Insufficiency
Authors: King Julia H. , Kwan Sze Ting (Cecilia) , Yan Jian , Jiang Xinyin , Fomin Vladislav G. , Levine Samantha P. , Wei Emily , Roberson Mark S. , Caudill Marie A.
Published: February 2019

Dlx3 (distal-less homeobox 3) haploinsufficiency in mice has been shown to result in restricted fetal growth and placental defects. We previously showed that maternal choline supplementation (4X versus 1X choline) in the Dlx3+/− mouse increased fetal and placental growth in mid-gestation. The current study sought to test the hypothesis that prenatal choline would modulate indicators of placenta function and development. Pregnant Dlx3+/− mice consuming 1X (control), 2X, or 4X choline from conception were sacrificed at embryonic (E) days E10.5, E12.5, E15.5, and E18.5, and placentas and embryos were harvested. Data were analyzed separately for each gestational day controlling for litter size, fetal genotype (except for models including only +/− pups), and fetal sex (except when data were stratified by this variable). 4X choline tended to increase (p < 0.1) placental labyrinth size at E10.5 and decrease (p < 0.05) placental apoptosis at E12.5. Choline supplementation decreased (p < 0.05) expression of pro-angiogenic genes Eng (E10.5, E12.5, and E15.5), and Vegf (E12.5, E15.5); and pro-inflammatory genes Il1b (at E15.5 and 18.5), Tnfα (at E12.5) and Nfκb (at E15.5) in a fetal sex-dependent manner. These findings provide support for a modulatory effect of maternal choline supplementation on biomarkers of placental function and development in a mouse model of placental insufficiency.
Mineral Nutrition and the Risk of Chronic Diseases: A Mendelian Randomization Study
Authors: Cheng Wen-Wen , Zhu Qiang , Zhang Hong-Yu
Published: February 2019

We applied Mendelian randomization analyses to investigate the potential causality between blood minerals (calcium, magnesium, iron, copper, and zinc) and osteoporosis (OP), gout, rheumatoid arthritis (RA), type 2 diabetes (T2D), Alzheimer’s disease (AD), bipolar disorder (BD), schizophrenia, Parkinson’s disease and major depressive disorder. Single nucleotide polymorphisms (SNPs) that are independent (r2 < 0.01) and are strongly related to minerals (p < 5 × 10−8) are selected as instrumental variables. Each standard deviation increase in magnesium (0.16 mmol/L) is associated with an 8.94-fold increase in the risk of RA (p = 0.044) and an 8.78-fold increase in BD (p = 0.040) but a 0.10 g/cm2 increase in bone density related to OP (p = 0.014). Each per-unit increase in copper is associated with a 0.87-fold increase in the risk of AD (p = 0.050) and BD (p = 0.010). In addition, there is suggestive evidence that calcium is positively correlated (OR = 1.36, p = 0.030) and iron is negatively correlated with T2D risk (OR = 0.89, p = 0.010); both magnesium (OR = 0.26, p = 0.013) and iron (OR = 0.71, p = 0.047) are negatively correlated with gout risk. In the sensitivity analysis, causal estimation is not affected by pleiotropy. This study supports the long-standing hypothesis that magnesium supplementation can increase RA and BD risks and decrease OP risk and that copper intake can reduce AD and BD risks. This study will be helpful to address some controversial debates on the relationships between minerals and chronic diseases.
Improved Morphine-Loaded Hydrogels for Wound-Related Pain Relief
Authors: Mateus Dinis , Marto Joana , Trindade Patrícia , Gonçalves Humberto , Salgado Ana , Machado Paula , Melo-Gouveia António , Ribeiro Helena M. , Almeida António J.
Published: February 2019

The use of morphine applied topically to painful wounds has potential advantages, such as dose reduction, fewer side effects and compound formulations, have been proposed for this purpose. Given the potential high impact of drug product quality on a patient’s health, the aim of the present study was to develop two stable sterile hydrogels containing morphine hydrochloride, intended for topical application on painful wounds. Two carboxymethylcellulose sodium-based hydrogels were prepared containing 0.125% w/w (F1-MH semi-solid formulation) and 1.0% w/w (F2-MH fluid formulation) morphine hydrochloride (MH), respectively. Studies included a risk assessment approach for definition of the quality target product profile (QTPP) and assessment of critical quality attributes (CQA) of the hydrogels to support product quality and safety. Safe, odourless, yellowish, translucent and homogeneous gels were obtained, with suitable microbiological and pharmaceutical characteristics. The active substance concentration was adapted according to the characteristics of the dose-metering device. Release profiles were investigated using Franz diffusion cells, and characterised by different kinetic models. Increasing gel viscosity prolonged drug release, with rates of 17.9 ± 2.2 μg·cm−2·h−1 (F1-MH) and 258.0 ± 30.4 μg·cm−2·h−1 (F2-MH), allowing for the reduction of the number of applications and improving patient compliance. The gels proved to be stable for up to 60 days at room temperature. The semi-solid and fluid MH-containing hydrogel formulations are safe, stable and suitable for use in hospital settings, which is rather important for wound-related pain management in cancer palliative care or burn patients.
PbCOP1.1 Contributes to the Negative Regulation of Anthocyanin Biosynthesis in Pear
Authors: Wu Meng , Si Min , Li Xieyu , Song Linyan , Liu Jianlong , Zhai Rui , Cong Liu , Yue Rongrong , Yang Chengquan , Ma Fengwang , Xu Lingfei , Wang Zhigang
Published: February 2019

The synthesis of anthocyanin in pear (Pyrus bretschneideri) fruit is regulated by light. However, little is known about the molecular mechanisms of pear fruit coloring mediated by upstream light-signaling regulators. Here, the photoresponse factors CONSTITUTIVE PHOTOMORPHOGENIC (COP) 1.1 and 1.2 were cloned from ‘Red Zaosu’ peel to study their functions in pear fruit coloring. The overexpression vectors pBI121-PbCOP1.1 and pBI121-PbCOP1.2 were constructed to analyze their effects on anthocyanin synthesis in pear fruit. A protein sequence alignment and phylogenetic tree analysis revealed that PbCOP1 proteins are highly homologous with those of other species. An analysis of tissue differential expression showed that the greatest expression levels of PbCOP1s occurred in the leaves. Their expression levels increased in the leaves during development, when the leaves changed from red to green. The overexpression of PbCOP1s in the peel resulted in reduced anthocyanin synthesis at the injection sites. A quantitative PCR analysis of the injection sites showed that PbCOP1.1 significantly inhibited the expression of the anthocyanin synthesis-related genes CHI, DFR, UFGT2, bHLH3, HY5 and GST. Based on the above results, we hypothesize that PbCOP1.1 is an anthocyanin synthetic inhibitory factor of pear coloration.
Traditional Medicine Plant, Onopordum acanthium L. (Asteraceae): Chemical Composition and Pharmacological Research
Authors: Garsiya Ekaterina Robertovna , Konovalov Dmitryi Alexeevich , Shamilov Arnold Alexeevich , Glushko Margarita Petrovna , Orynbasarova Kulpan Kenzhebaevna
Published: February 2019

For many years, plants have been used in the traditional medicine of different cultures. The biennial plant of the family Asteraceae, Onopordum acanthium L., also known as Scotch thistle, is used in traditional medicine as an anti-inflammatory, antitumor, and cardiotonic agent. The plant is widespread in the world; it grows in Europe and Asia and was introduced to America and Australia. Stems and buds of the first-year plant are used in cooking as an analogue of artichoke in European cuisine. Additionally, inflorescences contain a complex of proteolytic enzymes “onopordosin”, which may be used as a milk-clotting agent in the dairy industry. The chemical composition of the aerial part and roots of O. acanthium is represented by flavonoids, phenylpropanoids, lignans, triterpenoids, sesquiterpene lactones, and sterols. The anti-inflammatory, antiproliferative, and cardiotonic properties of the plant have been confirmed by pharmacological experiments with extracts and individual compounds using in silico, in vitro, and in vivo methods. This work is a review of information on the chemical composition and pharmacological studies of O. acanthium as a promising medicinal plant.
Nitric Oxide: Its Generation and Interactions with Other Reactive Signaling Compounds
Authors: Hancock John T. , Neill Steven J.
Published: February 2019

Nitric oxide (NO) is an immensely important signaling molecule in animals and plants. It is involved in plant reproduction, development, key physiological responses such as stomatal closure, and cell death. One of the controversies of NO metabolism in plants is the identification of enzymatic sources. Although there is little doubt that nitrate reductase (NR) is involved, the identification of a nitric oxide synthase (NOS)-like enzyme remains elusive, and it is becoming increasingly clear that such a protein does not exist in higher plants, even though homologues have been found in algae. Downstream from its production, NO can have several potential actions, but none of these will be in isolation from other reactive signaling molecules which have similar chemistry to NO. Therefore, NO metabolism will take place in an environment containing reactive oxygen species (ROS), hydrogen sulfide (H2S), glutathione, other antioxidants and within a reducing redox state. Direct reactions with NO are likely to produce new signaling molecules such as peroxynitrite and nitrosothiols, and it is probable that chemical competitions will exist which will determine the ultimate end result of signaling responses. How NO is generated in plants cells and how NO fits into this complex cellular environment needs to be understood.
Phytochemical Content of Melissa officinalis L. Herbal Preparations Appropriate for Consumption
Authors: Papoti Vassiliki T. , Totomis Nikolaos , Atmatzidou Aikaterini , Zinoviadou Kyriaki , Androulaki Anna , Petridis Dimitris , Ritzoulis Christos
Published: February 2019

Melissa Officinalis L. (MOL) domestic preparations appropriate for consumption were studied by monitoring content in Na, K, Ca, Li, phenolic bioactives (total phenols, hydroxycinnamic acid derivatives and flavonols), and antioxidant activity (1,1-diphenyl-2-picrylhydrazyl radical inhibition (DPPH) and ferric reducing ability (FRAP)). The effects of practice applied, material to solvent ratio, time of preparation, and solvent were studied. MOL decoctions and infusions, commonly prepared at home, were better or of equal nutritional value to preparations upon ultrasounds or maceration concerning the studied parameters. Aqueous MOL preparations were richer in total phenols (704–1949 mg per 250 mL) and the examined macroelements (1.1–2.9, 30.5–288.4 and 50.1–176.1 mg Na, K and Ca per 250 mL, respectively) and showed better antioxidant activity compared to ethanol counterparts. The 25% w/v hydroethanolic MOL preparations, suitable for consumption, presented a significant content in phenolic antioxidants and in the examined minerals, too. MOL infusions were significantly richer in total phenols with respective chamomile and olive leaf ones, comparatively examined. Overall acceptance scores of aqueous MOL preparations indicated that bitterness has to be masked for efficient reception by the consumers.
Sensor Fault-Tolerant Control Design for Mini Motion Package Electro-Hydraulic Actuator
Authors: Van Nguyen Tan , Ha Cheolkeun
Published: February 2019

With the rapid development of computer science and information and communication technology (ICT), increasingly intelligent, and complex systems have been applied to industries as well as human life. Fault-tolerant control (FTC) has, therefore, become one of the most important topics attracting attention from both engineers and researchers to maintain system performances when faults occur. The ultimate goal of this study was to develop a sensor fault-tolerant control (SFTC) to enhance the robust position tracking control of a class of electro-hydraulic actuators called mini motion packages (MMPs), which are widely used for applications requiring large force-displacement ratios. First, a mathematical model of the MMP system is presented, which is then applied in the position control process of the MMP system. Here, a well-known proportional, integrated and derivative (PID) control algorithm is employed to ensure the positional response to the reference position. Second, an unknown input observer (UIO) is designed to estimate the state vector and sensor faults using a linear matrix inequality (LMI) optimization algorithm. Then an SFTC is used to deal with sensor faults of the MMP system. The SFTC is formed of the fault detection and the fault compensation with the goal of determining the location, time of occurrence, and magnitude of the faults in the fault signal compensation process. Finally, numerical simulations were run to demonstrate the superior performance of the proposed approach compared to traditional tracking control.
Mapping the Mangrove Forest Canopy Using Spectral Unmixing of Very High Spatial Resolution Satellite Images
Authors: Taureau Florent , Robin Marc , Proisy Christophe , Fromard François , Imbert Daniel , Debaine Françoise
Published: February 2019

Despite the low tree diversity and scarcity of the understory vegetation, the high morphological plasticity of mangrove trees induces, at the stand level, a very large variability of forest structures that need to be mapped for assessing the functioning of such complex ecosystems. Fully constrained linear spectral unmixing (FCLSU) of very high spatial resolution (VHSR) multispectral images was tested to fine-scale map mangrove zonations in terms of horizontal variation of forest structure. The study was carried out on three Pleiades-1A satellite images covering French island territories located in the Atlantic, Indian, and Pacific Oceans, namely Guadeloupe, Mayotte, and New Caledonia archipelagos. In each image, FCLSU was trained from the delineation of areas exclusively related to four components including either pure vegetation, soil (ferns included), water, or shadows. It was then applied to the whole mangrove cover imaged for each island and yielded the respective contributions of those four components for each image pixel. On the forest stand scale, the results interestingly indicated a close correlation between FCLSU-derived vegetation fractions and canopy closure estimated from hemispherical photographs (R2 = 0.95) and a weak relation with the Normalized Difference Vegetation Index (R2 = 0.29). Classification of these fractions also offered the opportunity to detect and map horizontal patterns of mangrove structure in a given site. K-means classifications of fraction indeed showed a global view of mangrove structure organization in the three sites, complementary to the outputs obtained from spectral data analysis. Our findings suggest that the pixel intensity decomposition applied to VHSR multispectral satellite images can be a simple but valuable approach for (i) mangrove canopy monitoring and (ii) mangrove forest structure analysis in the perspective of assessing mangrove dynamics and productivity. As with Lidar-based surveys, these potential new mapping capabilities deserve further physically based interpretation of sunlight scattering mechanisms within forest canopy.