Abstract 2832: Comparison of bacteriome in plaque dental, saliva and tumor tissue in oral squamus carcinoma
Authors: Robayo Dabeiba A. García , Erira Herlinto Alveiro Tupaz , Jaimes Fredy Omar Gamboa 
Published: August 2019

Introduction: Bacterial microbiota found in the oral cavity, facilitates or promotes its carcinogenesis to Oral Squamous Cell Carcinoma (OSCC), by persistent inflammatory and production of acetaldehyde.

Objective: To determine and compare the microbiome and functional potential of dental plaque, saliva and tumor tissue of patients with OSCC with that found in dental plaque and saliva of healthy people.

Methods: After signed the informed consent 10 patients diagnosed with OSCC were selected. Samples were saliva, dental plaque and tumor tissue; sociodemographic data were collected from each patient, which were used to search for their corresponding control as age, gender, alcohol and tobacco consumptions. Subsequently, DNA was extracted from all the samples and 16S amplification and sequencing by ion-torrent. Once the data assembled paired-end reads were clustered into Operational Taxonomic Units (OTUs) algorithm with the open reference OTU picking pipeline in QIIME 1.9.1. The taxonomic assignment was Greengenes Database version 13.8. The α diversity and β diversity were calculated. To determine the metabolic potential of the oral microbiota, we used the PICRUSt significance was less 0.05.

Results: It was observed that the greatest bacterial diversity in dental plaque samples compared to the samples of saliva and tumor tissue. Multiple comparisons were made between the microbiome present between the different types of samples and between the two groups (cancer vs control). A total of 25 bacterial genera were found in exclusively in patients with OSCC and in more than 30% of patients who were Geobacillus, Anaerotruncus, Comamonas, Sphingomonas, Sphaerochaeta, Dorea, Delftia, Pseudoxanthomonas, Luteibacter, Pseudomonas, Peptoniphilus, Novosphingobium, Bradyrhizobium, Faecalibacterium, Clostridium, Dechloromonas, Methylobacterium, Proteus, Finegoldia, Enterococcus, Turicibacter, Desulfovibrio, Pyramidobacter, Morganella and Staphylococcus, and 2 those found exclusively in healthy people in more than 30% of controls that were Odoribacter and Helicobacter. Regarding the functional potential, statistically significant differences were observed between patients with OSCC and healthy people, increased ion-coupled transporter signaling pathways in patients with OSCC and the signaling pathways related to increased replication, recombination and repair in the group of healthy people.

Conclusions: Bacterial genera associated with OSCC and associated with health were described and propose them as non-invasive biomarkers. Additionally, signaling pathways increased by the microbiota associated with oral cancer are related to transporters associated with ions, while signaling pathways increased by the microbiota associated with health are related to replication, recombination and repair.

Citation Format: Dabeiba A. García Robayo, Herlinto Alveiro Tupaz Erira, Fredy Omar Gamboa Jaimes. Comparison of bacteriome in plaque dental, saliva and tumor tissue in oral squamus carcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 2832.