bluuebunny/crossref_metadata_2025 huggingface dataset:
Overexpression of Hepatocyte Chemerin-156 Lowers Tumor Burden in a Murine Model of Diethylnitrosamine-Induced Hepatocellular Carcinoma
Authors: Haberl Elisabeth M. , Pohl Rebekka , Rein-Fischboeck Lisa , Feder Susanne , Sinal Christopher J. , Bruckmann Astrid , Hoering Marcus , Krautbauer Sabrina , Liebisch Gerhard , Buechler Christa
Published: January 2020

The tumor inhibitory potential of the highly active chemerin-156 isoform was described in orthotopic models of hepatocellular carcinoma (HCC). The majority of HCC arises in the fibrotic liver, which was not reproduced in these studies. Here, a potential therapeutic activity of chemerin-156 was evaluated in diethylnitrosamine (DEN)-induced liver cancer, which mimics fibrosis-associated HCC. Mice were infected with adeno-associated virus (AAV) six months after DEN injection to overexpress chemerin-156 in the liver, and animals injected with non-recombinant-AAV served as controls. Three months later, the animals were killed. Both groups were comparable with regard to liver steatosis and fibrosis. Of note, the number of very small tumors was reduced by chemerin-156. Anyhow, the expression of inflammatory and profibrotic genes was similar in larger tumors of control and chemerin-156-AAV-infected animals. Although genes with a role in lipid metabolism, like 3-hydroxy-3-methylglutaryl-coenzym-A--reductase, were overexpressed in tumors of animals with high chemerin-156, total hepatic cholesterol, diacylglycerol and triglyceride levels, and distribution of individual lipid species were normal. Chemerin-156-AAV-infected mice had elevated hepatic and systemic chemerin. Ex vivo activation of the chemerin receptor chemokine-like receptor 1 increased in parallel with serum chemerin, illustrating the biological activity of the recombinant protein. In the tumors, chemerin-155 was the most abundant variant. Chemerin-156 was not detected in tumors of the controls and was hardly found in chemerin-156-AAV infected animals. In conclusion, the present study showed that chemerin-156 overexpression caused a decline in the number of small lesions but did not prevent the growth of pre-existing neoplasms.
Emerging Role of Non-Coding RNAs in Esophageal Squamous Cell Carcinoma
Authors: Feng Qingqing , Zhang Hongli , Yao Denglin , Chen Wei-Dong , Wang Yan-Dong
Published: January 2020

Esophageal squamous cell carcinoma (ESCC) is a highly prevalent tumor and is associated with ethnicity, genetics, and dietary intake. Non-coding RNAs (ncRNAs), specifically microRNAs (miRNAs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs) have been reported as functional regulatory molecules involved in the development of many human cancers, including ESCC. Recently, several ncRNAs have been detected as oncogenes or tumor suppressors in ESCC progression. These ncRNAs influence the expression of specific genes or their associated signaling pathways. Moreover, interactions of ncRNAs are evident in ESCC, as miRNAs regulate the expression of lncRNAs, and further, lncRNAs and circRNAs function as miRNA sponges to compete with the endogenous RNAs. Here, we discuss and summarize the findings of recent investigations into the role of ncRNAs (miRNAs, lncRNAs, and circRNAs) in the development and progression of ESCC and how their interactions regulate ESCC development.
Cryptotanshinone from the Salvia miltiorrhiza Bunge Attenuates Ethanol-Induced Liver Injury by Activation of AMPK/SIRT1 and Nrf2 Signaling Pathways
Authors: Nagappan Arulkumar , Kim Ji-Hyun , Jung Dae Young , Jung Myeong Ho
Published: January 2020

Cryptotanshinone (CT), a diterpene that is isolated from Salvia miltiorrhiza Bunge, exhibits anti-cancer, anti-oxidative, anti-fibrosis, and anti-inflammatory properties. Here, we examined whether CT administration possess a hepatoprotective effect on chronic ethanol-induced liver injury. We established a chronic alcohol feeding mouse model while using C57BL/6 mice, and examined the liver sections with hematoxylin-eosin (H&E) and Oil Red O (ORO) staining. Further, we analyzed the lipogenesis, fatty acid oxidation, oxidative stress, and inflammation genes by using quantitative polymerase chain reaction (qPCR) and immunoblotting in in vivo, and in vitro while using HepG2 and AML-12 cells. CT treatment significantly ameliorated ethanol-promoted hepatic steatosis, which was consistent with the decreased hepatic triglyceride levels. Interestingly, CT activated the phosphorylation of AMP-activated protein kinase (AMPK), sirtuin 1 (SIRT1), and nuclear factor E2-related factor 2 (Nrf2) proteins. Importantly, compound C (AMPK inhibitor) significantly blocked the CT-mediated reduction in TG accumulation, but not Ex52735 (SIRT1 inhibitor), which suggested that CT countering ethanol-promoted hepatic steatosis is mediated by AMPK activation. Furthermore, CT significantly inhibited cytochrome P450 2E1 (CYP2E1) and enhanced both the expression of antioxidant genes and hepatic glutathione levels. Finally, CT inhibited the ethanol-induced inflammation in ethanol-fed mice and HepG2 cells. Overall, CT exhibits a hepatoprotective effect against ethanol-induced liver injury by the inhibition of lipogenesis, oxidative stress, and inflammation through the activation of AMPK/SIRT1 and Nrf2 and the inhibition of CYP2E1. Therefore, CT could be an effective therapeutic agent for treating ethanol-induced liver injury.
Neutrophil Metabolic Shift during Their Lifecycle: Impact on Their Survival and Activation
Authors: Injarabian Louise , Devin Anne , Ransac Stéphane , Marteyn Benoit S.
Published: January 2020

Polymorphonuclear neutrophils (PMNs) are innate immune cells, which represent 50% to 70% of the total circulating leukocytes. How PMNs adapt to various microenvironments encountered during their life cycle, from the bone marrow, to the blood plasma fraction, and to inflamed or infected tissues remains largely unexplored. Metabolic shifts have been reported in other immune cells such as macrophages or lymphocytes, in response to local changes in their microenvironment, and in association with a modulation of their pro-inflammatory or anti-inflammatory functions. The potential contribution of metabolic shifts in the modulation of neutrophil activation or survival is anticipated even though it is not yet fully described. If neutrophils are considered to be mainly glycolytic, the relative importance of alternative metabolic pathways, such as the pentose phosphate pathway, glutaminolysis, or the mitochondrial oxidative metabolism, has not been fully considered during activation. This statement may be explained by the lack of knowledge regarding the local availability of key metabolites such as glucose, glutamine, and substrates, such as oxygen from the bone marrow to inflamed tissues. As highlighted in this review, the link between specific metabolic pathways and neutrophil activation has been outlined in many reports. However, the impact of neutrophil activation on metabolic shifts’ induction has not yet been explored. Beyond its importance in neutrophil survival capacity in response to available metabolites, metabolic shifts may also contribute to neutrophil population heterogeneity reported in cancer (tumor-associated neutrophil) or auto-immune diseases (Low/High Density Neutrophils). This represents an active field of research. In conclusion, the characterization of neutrophil metabolic shifts is an emerging field that may provide important knowledge on neutrophil physiology and activation modulation. The related question of microenvironmental changes occurring during inflammation, to which neutrophils will respond to, will have to be addressed to fully appreciate the importance of neutrophil metabolic shifts in inflammatory diseases.
Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets
Authors: Alshevskaya Alina , Lopatnikova Julia , Zhukova Julia , Chumasova Oksana , Shkaruba Nadezhda , Sizikov Aleksey , Evsegneeva Irina , Gladkikh Victor , Karaulov Aleksander , Sennikov Sergey V.
Published: January 2020

Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, and by number, density, and co-expression of its membrane-bound receptors type 1 and 2 (TNFR1 and TNFR2). The aim of this study was to reveal associations between TNFR1/2 co-expression profile parameters and RA disease activity indicators. Methods: PBMC were analyzed from 46 healthy donors and 64 patients with RA using flow cytometry. Patients were divided according to the disease activity score (DAS) 28 index into groups with high (n = 22, 34.4%), moderate (n = 30, 46.9%), and low (n = 12, 18.8%) disease activity. Co-expression of TNFR1 and TNFR2 was studied by evaluating the percentage of cells, with different receptors, and by counting the number of receptors of each type per cell, using QuantiBritePE beads. Associations between disease severity and activity indicators and parameters of TNFα receptor expression in subpopulations of immune cells were studied. Results: T cell subsets from RA patients were characterized by co-expression of TNFR1 and TNFR2, and were found to differ significantly compared with healthy donors. Memory cells both among T helper cells and cytotoxic T cells demonstrated the most significant differences in TNFR-expression profile. Multivariable logistic regression revealed model to identified RA patients from healthy individual based on the TNFR1/2 co-expression parameters. Conclusion: The profile of TNFR1\2 co-expression differs in RA comparing with health. Proportion of TNFR1+TNFR2- cells increased significantly among memory T helper cells and activated cytotoxic T cells, and decreased significantly among naïve cytotoxic T cells and T regulatory cells as compared with health. The parameters of TNFR1\2 co-expression in RA are associated with clinical and laboratory indicators of disease activity.
Identification of Novel Pathways Associated with Patterned Cerebellar Purkinje Neuron Degeneration in Niemann-Pick Disease, Type C1
Authors: Martin Kyle B. , Williams Ian M. , Cluzeau Celine V. , Cougnoux Antony , Dale Ryan K. , Iben James R. , Cawley Niamh X. , Wassif Christopher A. , Porter Forbes D.
Published: January 2020

Niemann-Pick disease, type C1 (NPC1) is a lysosomal disease characterized by progressive cerebellar ataxia. In NPC1, a defect in cholesterol transport leads to endolysosomal storage of cholesterol and decreased cholesterol bioavailability. Purkinje neurons are sensitive to the loss of NPC1 function. However, degeneration of Purkinje neurons is not uniform. They are typically lost in an anterior-to-posterior gradient with neurons in lobule X being resistant to neurodegeneration. To gain mechanistic insight into factors that protect or potentiate Purkinje neuron loss, we compared RNA expression in cerebellar lobules III, VI, and X from control and mutant mice. An unexpected finding was that the gene expression differences between lobules III/VI and X were more pronounced than those observed between mutant and control mice. Functional analysis of genes with anterior to posterior gene expression differences revealed an enrichment of genes related to neuronal cell survival within the posterior cerebellum. This finding is consistent with the observation, in multiple diseases, that posterior Purkinje neurons are, in general, resistant to neurodegeneration. To our knowledge, this is the first study to evaluate anterior to posterior transcriptome-wide changes in gene expression in the cerebellum. Our data can be used to not only explore potential pathological mechanisms in NPC1, but also to further understand cerebellar biology.
Docker4Circ: A Framework for the Reproducible Characterization of circRNAs from RNA-Seq Data
Authors: Ferrero Giulio , Licheri Nicola , Coscujuela Tarrero Lucia , De Intinis Carlo , Miano Valentina , Calogero Raffaele Adolfo , Cordero Francesca , De Bortoli Michele , Beccuti Marco
Published: January 2020

Recent improvements in cost-effectiveness of high-throughput technologies has allowed RNA sequencing of total transcriptomes suitable for evaluating the expression and regulation of circRNAs, a relatively novel class of transcript isoforms with suggested roles in transcriptional and post-transcriptional gene expression regulation, as well as their possible use as biomarkers, due to their deregulation in various human diseases. A limited number of integrated workflows exists for prediction, characterization, and differential expression analysis of circRNAs, none of them complying with computational reproducibility requirements. We developed Docker4Circ for the complete analysis of circRNAs from RNA-Seq data. Docker4Circ runs a comprehensive analysis of circRNAs in human and model organisms, including: circRNAs prediction; classification and annotation using six public databases; back-splice sequence reconstruction; internal alternative splicing of circularizing exons; alignment-free circRNAs quantification from RNA-Seq reads; and differential expression analysis. Docker4Circ makes circRNAs analysis easier and more accessible thanks to: (i) its R interface; (ii) encapsulation of computational tasks into docker images; (iii) user-friendly Java GUI Interface availability; and (iv) no need of advanced bash scripting skills for correct use. Furthermore, Docker4Circ ensures a reproducible analysis since all its tasks are embedded into a docker image following the guidelines provided by Reproducible Bioinformatics Project.
Granulosa-Lutein Cell Sirtuin Gene Expression Profiles Differ between Normal Donors and Infertile Women
Authors: González-Fernández Rebeca , Martín-Ramírez Rita , Rotoli Deborah , Hernández Jairo , Naftolin Frederick , Martín-Vasallo Pablo , Palumbo Angela , Ávila Julio
Published: January 2020

Sirtuins are a family of deacetylases that modify structural proteins, metabolic enzymes, and histones to change cellular protein localization and function. In mammals, there are seven sirtuins involved in processes like oxidative stress or metabolic homeostasis associated with aging, degeneration or cancer. We studied gene expression of sirtuins by qRT-PCR in human mural granulosa-lutein cells (hGL) from IVF patients in different infertility diagnostic groups and in oocyte donors (OD; control group). Study 1: sirtuins genes’ expression levels and correlations with age and IVF parameters in women with no ovarian factor. We found significantly higher expression levels of SIRT1, SIRT2 and SIRT5 in patients ≥40 years old than in OD and in women between 27 and 39 years old with tubal or male factor, and no ovarian factor (NOF). Only SIRT2, SIRT5 and SIRT7 expression correlated with age. Study 2: sirtuin genes’ expression in women poor responders (PR), endometriosis (EM) and polycystic ovarian syndrome. Compared to NOF controls, we found higher SIRT2 gene expression in all diagnostic groups while SIRT3, SIRT5, SIRT6 and SIRT7 expression were higher only in PR. Related to clinical parameters SIRT1, SIRT6 and SIRT7 correlate positively with FSH and LH doses administered in EM patients. The number of mature oocytes retrieved in PR is positively correlated with the expression levels of SIRT3, SIRT4 and SIRT5. These data suggest that cellular physiopathology in PR’s follicle may be associated with cumulative DNA damage, indicating that further studies are necessary.
Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes
Authors: Singh Rupesh Kumar , Martins Viviana , Soares Bruno , Castro Isaura , Falco Virgílio
Published: January 2020

Despite the numerous beneficial properties and uses of chitosan in agriculture, the molecular mechanisms behind its elicitation potential are still unclear. This study aimed at understanding the effect of chitosan application in the levels of phenolic compounds of Vitis vinifera L. red grapes berry skin (cv. Tinto Cão) during veraison. Grapevines were treated with chitosan (0.01% in 0.01% acetic acid) while control grapevines were sprayed with 0.01% acetic acid. Results showed that several monomeric anthocyanins increased significantly in berry skins after treatment with chitosan. Additionally, Catechin, Rutin and Querecetin-3-O-galactoside were also recorded in higher amount upon chitosan treatment. Besides modulating the phenolic content, chitosan treatment also induced modifications in several target genes encoding key enzymes and transporters involved in secondary metabolic pathways. For instance, the genes PAL, CHS, F3H, ANR, UFGT, ABCC1, GST, MATE1 were upregulated in leaves and berry skins at veraison cessation in response to chitosan treatment. Overall, the results demonstrated that chitosan has a stimulatory effect on the accumulation of phenolic compounds, including anthocyanins, mediated by modifications in the transcription of key genes involved in their biosynthesis and transport in grape berries.
In Situ Proteolysis Condition-Induced Crystallization of the XcpVWX Complex in Different Lattices
Authors: Zhang Yichen , Wang Shu , Jia Zongchao
Published: January 2020

Although prevalent in the determination of protein structures; crystallography always has the bottleneck of obtaining high-quality protein crystals for characterizing a wide range of proteins; especially large protein complexes. Stable fragments or domains of proteins are more readily to crystallize; which prompts the use of in situ proteolysis to remove flexible or unstable structures for improving crystallization and crystal quality. In this work; we investigated the effects of in situ proteolysis by chymotrypsin on the crystallization of the XcpVWX complex from the Type II secretion system of Pseudomonas aeruginosa. Different proteolysis conditions were found to result in two distinct lattices in the same crystallization solution. With a shorter chymotrypsin digestion at a lower concentration; the crystals exhibited a P3 hexagonal lattice that accommodates three complex molecules in one asymmetric unit. By contrast; a longer digestion with chymotrypsin of a 10-fold higher concentration facilitated the formation of a compact P212121 orthorhombic lattice with only one complex molecule in each asymmetric unit. The molecules in the hexagonal lattice have shown high atomic displacement parameter values compared with the ones in the orthorhombic lattice. Taken together; our results clearly demonstrate that different proteolysis conditions can result in the generation of distinct lattices in the same crystallization solution; which can be exploited in order to obtain different crystal forms of a better quality
Non-Coding RNAs Participate in the Regulation of CRY-DASH in the Growth and Early Development of Saccharina japonica (Laminariales, Phaeophyceae)
Authors: Yang Xiaoqi , Li Lu , Wang Xiuliang , Yao Jianting , Duan Delin
Published: January 2020

CRY-DASH, a new cryptochrome blue light receptor, can repair damaged DNA and regulate secondary metabolism and development of fungus. However, its role in regulation during the growth of Saccharina japonica is still unclear. After cloning the full-length of CRY-DASH from S. japonica (sjCRY-DASH), we deduced that its open reading frame was 1779 bp long and encoded 592 amino acids. sjCRY-DASH transcription was rapidly upregulated within 30 min in response to blue light and exhibited 24 h periodicity with different photoperiods. Moreover, sjCRY-DASH maintained the same periodicity in suitable growth temperature, suggesting a close relationship between this periodicity and circadian rhythm regulation. Novel-m3234-5p, which was targeted to sjCRY-DASH, decreased with increasing sjCRY-DASH transcription, acting as a negative modulator of sjCRY-DASH. Six long non-coding RNAs classified as long intergenic non-coding RNAs (lincRNAs) exhibited co-expression with sjCRY-DASH. A miRNA sjCRY DASH lincRNA network was consequently identified. By predicting the endogenous competing mRNAs of novel-m3234-5p, we found that sjCRY-DASH indirectly participated in the regulation of DNA damage repair, protein synthesis and processing, and actin transport. In conclusion, our results revealed that non-coding RNAs participate in the regulation of sjCRY-DASH, which played vital roles in the growth and early development of S. japonica.
GSK-3β-Targeting Fisetin Promotes Melanogenesis in B16F10 Melanoma Cells and Zebrafish Larvae through β-Catenin Activation
Authors: Molagoda Ilandarage Menu Neelaka , Karunarathne Wisurumuni Arachchilage Hasitha Maduranga , Park Sang Rul , Choi Yung Hyun , Park Eui Kyun , Jin Cheng-Yun , Yu Haiyang , Jo Wol Soon , Lee Kyoung Tae , Kim Gi-Young
Published: January 2020

Fisetin is found in many fruits and plants such as grapes and onions, and exerts anti-inflammatory, anti-proliferative, and anticancer activity. However, whether fisetin regulates melanogenesis has been rarely studied. Therefore, we evaluated the effects of fisetin on melanogenesis in B16F10 melanoma cell and zebrafish larvae. The current study revealed that fisetin slightly suppressed in vitro mushroom tyrosinase activity; however, molecular docking data showed that fisetin did not directly bind to mushroom tyrosinase. Unexpectedly, fisetin significantly increased intracellular and extracellular melanin production in B16F10 melanoma cells regardless of the presence or absence of α-melanocyte stimulating hormone (α-MSH). We also found that the expression of melanogenesis-related genes such as tyrosinase and microphthalmia-associated transcription factor (MITF), were highly increased 48 h after fisetin treatment. Pigmentation of zebrafish larvae by fisetin treatment also increased at the concentrations up to 200 µM and then slightly decreased at 400 µM, with no alteration in the heart rates. Molecular docking data also revealed that fisetin binds to glycogen synthase kinase-3β (GSK-3β). Therefore, we evaluated whether fisetin negatively regulated GSK-3β, which subsequently activates β-catenin, resulting in melanogenesis. As expected, fisetin increased the expression of β-catenin, which was subsequently translocated into the nucleus. In the functional assay, FH535, a Wnt/β-catenin inhibitor, significantly inhibited fisetin-mediated melanogenesis in zebrafish larvae. Our data suggested that fisetin inhibits GSK-3β, which activates β-catenin, resulting in melanogenesis through the revitalization of MITF and tyrosinase.
Conformational Plasticity of the Active Site Entrance in E. coli Aspartate Transcarbamoylase and Its Implication in Feedback Regulation
Authors: Lei Zhen , Wang Nan , Tan Hongwei , Zheng Jimin , Jia Zongchao
Published: January 2020

Aspartate transcarbamoylase (ATCase) has been studied for decades and Escherichia coli ATCase is referred as a “textbook example” for both feedback regulation and cooperativity. However, several critical questions about the catalytic and regulatory mechanisms of E. coli ATCase remain unanswered, especially about its remote feedback regulation. Herein, we determined a structure of E. coli ATCase in which a key residue located (Arg167) at the entrance of the active site adopted an uncommon open conformation, representing the first wild-type apo-form E. coli ATCase holoenzyme that features this state. Based on the structure and our results of enzymatic characterization, as well as molecular dynamic simulations, we provide new insights into the feedback regulation of E. coli ATCase. We speculate that the binding of pyrimidines or purines would affect the hydrogen bond network at the interface of the catalytic and regulatory subunit, which would further influence the stability of the open conformation of Arg167 and the enzymatic activity of ATCase. Our results not only revealed the importance of the previously unappreciated open conformation of Arg167 in the active site, but also helped to provide rationalization for the mechanism of the remote feedback regulation of ATCase.
Protein Stability and Functional Characterization of Intra-Melanosomal Domain of Human Recombinant Tyrosinase-Related Protein 1
Authors: Dolinska Monika B. , Young Kenneth L. , Kassouf Claudia , Dimitriadis Emilios K. , Wingfield Paul T. , Sergeev Yuri V.
Published: January 2020

Pigmentation is the result of a complex process by which the biopolymer melanin is synthesized and packed into melanosomes of melanocytes. Various types of oculocutaneous albinism (OCA), a series of autosomal recessive disorders, are associated with reduced pigmentation in the skin, eyes, and hair due to genetic mutations of proteins involved in melanogenesis. Human tyrosinase (Tyr) and tyrosinase-related protein 1 (Tyrp1) drives the enzymatic process of pigment bio-polymerization. However, within the melanogenic pathway, Tyrp1 has catalytic functions not clearly defined and distinct from Tyr. Here, we characterize the biochemical and biophysical properties of recombinant human Tyrp1. For this purpose, we purified and analyzed the intra-melanosomal domain (Tyrp1tr) for protein stability and enzymatic function in conditions mimicking the environment within melanosomes and the endoplasmic reticulum. The study suggests that Tyrp1tr is a monomeric molecule at ambient temperatures and below (<25 °C). At higher temperatures, >31 °C, higher protein aggregates form with a concurrent decrease of monomers in solution. Also, Tyrp1tr diphenol oxidase activity at pH 5.5 rises as both the pre-incubation temperature and the higher molecular weight protein aggregates formation increases. The enhanced protein activity is consistent with the volume exclusion change caused by protein aggregates.
Network Medicine Approach for Analysis of Alzheimer’s Disease Gene Expression Data
Authors: Cohen David , Pilozzi Alexander , Huang Xudong
Published: January 2020

Alzheimer’s disease (AD) is the most widespread diagnosed cause of dementia in the elderly. It is a progressive neurodegenerative disease that causes memory loss as well as other detrimental symptoms that are ultimately fatal. Due to the urgent nature of this disease, and the current lack of success in treatment and prevention, it is vital that different methods and approaches are applied to its study in order to better understand its underlying mechanisms. To this end, we have conducted network-based gene co-expression analysis on data from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database. By processing and filtering gene expression data taken from the blood samples of subjects with varying disease states and constructing networks based on that data to evaluate gene relationships, we have been able to learn about gene expression correlated with the disease, and we have identified several areas of potential research interest.
Gluconacetobacter diazotrophicus Changes The Molecular Mechanisms of Root Development in Oryza sativa L. Growing Under Water Stress
Authors: Silva Renata , Filgueiras Luanna , Santos Bruna , Coelho Mariana , Silva Maria , Estrada-Bonilla Germán , Vidal Marcia , Baldani José Ivo , Meneses Carlos
Published: January 2020

Background: Inoculation with Gluconacetobacter diazotrophicus has shown to influence root development in red rice plants, and more recently, the induced systemic tolerance (IST) response to drought was also demonstrated. The goal of this study was to evaluate the inoculation effect of G. diazotrophicus strain Pal5 on the amelioration of drought stress and root development in red rice (Oryza sativa L.). Methods: The experimental treatments consist of red rice plants inoculated with and without strain Pal5 in presence and absence of water restriction. Physiological, biochemical, and molecular analyses of plant roots were carried out, along with measurements of growth and biochemical components. Results: The plants showed a positive response to the bacterial inoculation, with root growth promotion and induction of tolerance to drought. An increase in the root area and higher levels of osmoprotectant solutes were observed in roots. Bacterial inoculation increased the drought tolerance and positively regulated certain root development genes against the water deficit in plants. Conclusion: G. diazotrophicus Pal5 strain inoculation favored red rice plants by promoting various root growth and developmental mechanisms against drought stress, enabling root development and improving biochemical composition.
A Parameter-Free Outlier Detection Algorithm Based on Dataset Optimization Method
Authors: Wang Liying , Shi Lei , Xu Liancheng , Liu Peiyu , Zhang Lindong , Dong Yanru
Published: January 2020

Recently, outlier detection has widespread applications in different areas. The task is to identify outliers in the dataset and extract potential information. The existing outlier detection algorithms mainly do not solve the problems of parameter selection and high computational cost, which leaves enough room for further improvements. To solve the above problems, our paper proposes a parameter-free outlier detection algorithm based on dataset optimization method. Firstly, we propose a dataset optimization method (DOM), which initializes the original dataset in which density is greater than a specific threshold. In this method, we propose the concepts of partition function (P) and threshold function (T). Secondly, we establish a parameter-free outlier detection method. Similarly, we propose the concept of the number of residual neighbors, as the number of residual neighbors and the size of data clusters are used as the basis of outlier detection to obtain a more accurate outlier set. Finally, extensive experiments are carried out on a variety of datasets and experimental results show that our method performs well in terms of the efficiency of outlier detection and time complexity.
Smart Thermomechanochemical Composite Materials Driven by Different Forms of Electromagnetic Radiation
Authors: Riberi Kevin , Bongiovanni Abel Silvestre , Martinez María V. , Molina María A. , Rivarola Claudia R. , Acevedo Diego F. , Rivero Rebeca , Cuello Emma Antonia , Gramaglia Romina , Barbero Cesar A.
Published: January 2020

Photo-thermo-mechanochemical (P-T-MCh) nanocomposites provide a mechanical and/or chemical output (MCh) in response to a photonic (P) input, with the thermal (T) flux being the coupling factor. The nanocomposite combines a photon absorbing nanomaterial with a thermosensitive hydrogel matrix. Conjugated (absorbing in the near infrared (NIR, 750–850 nm) wavelength range) polymer (polyaniline, PANI) nanostructures are dispersed in cross-linked thermosensitive (poly(N-isopropylacrylamide), PNIPAM) hydrogel matrices, giving the nanocomposite P-T-MCh properties. Since PANI is a conductive polymer, electromagnetic radiation (ER) such as radiofrequency (30 kHz) and microwaves (2.4 GHz) could also be used as an input. The alternating electromagnetic field creates eddy currents in the PANI, which produces heat through the Joule effect. A new kind of “product” nanocomposite is then produced, where ER drives the mechanochemical properties of the material through thermal coupling (electromagnetic radiation thermomechanochemical, ER-T-MCh). Both optical absorption and conductivity of PANI depend on its oxidation and protonation state. Therefore, the ER-T-MCh materials are able to react to the surroundings properties (pH, redox potential) becoming a smart (electromagnetic radiation thermomechanochemical) (sER-T-MCh) material. The volume changes of the sER-T-MCh materials are reversible since the size and shape is recovered by cooling. No noticeable damage was observed after several cycles. The mechanical properties of the composite materials can be set by changing the hydrogel matrix. Four methods of material fabrication are described.
Phenolic Resin Foam Composites Reinforced by Acetylated Poplar Fiber with High Mechanical Properties, Low Pulverization Ratio, and Good Thermal Insulation and Flame Retardant Performance
Authors: Liu Jian , Wang Liuliu , Zhang Wei , Han Yanming
Published: January 2020

Phenolic foam composites (PFs) are of substantial interest due to their uniform closed-cell structure, low thermal conductivity, and good thermal insulation performance. However, their disadvantages of a high pulverization rate and poor mechanical properties restrict their application in building exterior insulation. Therefore, the toughening of these composites is necessary. In this paper, poplar fiber was treated with an acetylation reagent, and the acetylated fiber was used to prepare modified phenolic foams (FTPFs); this successfully solved the phenomenon of the destruction of the foam structure due to the agglomeration of poplar fiber in the resin substrate. The foam composites were comprehensively evaluated via the characterization of their chemical structures, surface morphologies, mechanical properties, thermal conductivities, and flame retardant properties. It was found that the compressive strength and compressive modulus of FTPF-5% respectively increased by 28.5% and 37.9% as compared with those of PF. The pulverization ratio was reduced by 32.3%, and the thermal insulation performance and flame retardant performance (LOI) were improved. Compared with other toughening methods for phenolic foam composites, the phenolic foam composites modified with surface-compatibilized poplar fiber offer a novel strategy for the value-added utilization of woody fiber, and improve the toughness and industrial viability of phenolic foam.
Effects of Functionalized Nano-TiO2 on the Molecular Motion in Epoxy Resin-Based Nanocomposites
Authors: Wang Shihang , Yu Shihu , Li Jianying , Li Shengtao
Published: January 2020

Epoxy resin-based nanocomposites have been widely researched for being potential insulating materials in high voltage power equipment. In this paper, nano-TiO2 particles were chosen and surface-modified by a silane coupling agent containing an epoxy group. The effect of functionalized nano-TiO2 doping on the physical properties of epoxy resin was studied. The results of differential scanning calorimetry show that Tg increased significantly and can be increased by up to 35 °C. Therefore, it is believed that the suppression of molecular motion by the addition of nanofillers works effectively in the case of this functionalized nano-TiO2 and a strong interaction between the epoxy resin and the nano-TiO2 was formed after surface modification. Consequently, dynamic mechanical properties, thermal conductivity, electrical conductivity, and trap characteristics of epoxy resin are all adjusted after introducing functionalized nano-TiO2. All of these physical properties were analyzed from the perspective of suppression of molecular motion, and it is of significance to establish the theory of a nanocomposite dielectric. Besides, the results show that the epoxy/TiO2 nanocomposite is expected to be applied in the insulation system of electrical equipment.
Material Characterization of PMC/TBC Composite Under High Strain Rates and Elevated Temperatures
Authors: Golewski Przemysław , Rusinek Alexis , Sadowski Tomasz
Published: January 2020

Polymer matrix composites (PMC), despite their many advantages, have limited use at elevated temperatures. To expand the scope of their uses, it becomes necessary to use thermal barrier coatings (TBC). In addition to elevated temperatures, composite structures, and thus TBC barriers, can be exposed to damage from impacts of foreign objects. Therefore, before using the thermal barrier in practice, knowledge about its behavior under high-speed loads is necessary. The paper presents results for samples with the PMC/TBC system subjected to dynamic compression using a split Hopkinson pressure bar (SHPB). The substrate was made of CFRP (carbon reinforced polymer) with epoxy matrix and twill fabric. TBC was made of ceramic mat saturated by commercial hardener from Vitcas company. The tests were carried out at ambient temperature and elevated temperature—55 °C and 90 °C. Tests at ambient temperature were carried out for three pressure levels: 1, 1.5, and 2 bar. Only the pressure of 1 bar was used for the elevated temperature. Studies have shown that the limit load is 1 bar for ambient temperature. At 1.5 bar, cracks occurred in the TBC structure. Increased temperature also adversely affects the TBC barrier strength and it is damaged at a pressure of 1 bar.
Searching for Rheological Conditions for FFF 3D Printing with PVC Based Flexible Compounds
Authors: Calafel I. , Aguirresarobe R. H. , Peñas M. I. , Santamaria A. , Tierno M. , Conde J. I. , Pascual B.
Published: January 2020

Rheology is proposed as a tool to explore plasticized poly(vinyl chloride) (PVC) formulations to be used in the fused filament fabrication (FFF) 3D printing process and so manufactures flexible and ductile objects by this technique. The viscoelastic origin of success/failure in FFF of these materials is investigated. The analysis of buckling of the filament is based on the ratio between compression modulus and viscosity, but for a correct approach the viscosity should be obtained under the conditions established in the nozzle. As demonstrated by small amplitude oscillatory shear (SAOS) measurements, PVC formulations have a crystallites network that provokes clogging in the nozzle. This network restricts printing conditions, because only vanishes at high temperatures, at which thermal degradation is triggered. It is observed that the analysis of the relaxation modulus G(t) is more performing than the G″/G′ ratio to get conclusions on the quality of layers welding. Models printed according to the established conditions show an excellent appearance and flexibility, marking a milestone in the route to obtain flexible objects by FFF.
High Specific Capacitance of the Electrodeposited MnO2 on Porous Foam Nickel Soaked in Alcohol and its Dependence on Precursor Concentration
Authors: Zhang Ming , Dai Xiaoli , Zhang Cuixian , Fuan Yuanwu , Yang Dingyu , Li Jitao
Published: January 2020

In this work, we used the mixed solution of manganese acetate and sodium sulfate to deposit manganese dioxide on the three-dimensional porous nickel foam that was previously soaked in alcohol, and then the effects of solution concentrations on their capacitance properties were investigated. The surface morphology, microstructure, elemental valence and other information of the material were observed by scanning electron microscope (SEM), Transmission Electron Microscope (TEM), X-ray photoelectron spectroscopy (XPS), etc. The electrochemical properties of the material were tested by Galvanostatic charge-discharge (GCD), Cyclic Voltammetry (CV), Chronoamperometry (CA), Electrochemical impedance spectroscopy (EIS), etc. The MnO2 electrode prepared at lower concentrations can respectively reach a specific capacitance of 529.5 F g−1 and 237.3 F g−1 at the current density of 1 A g−1 and 10 A g−1, and after 2000 cycles, the capacity retention rate was still 79.8% of the initial capacitance, and the energy density can even reach 59.4 Wh Kg−1, while at the same time, it also has a lower electrochemical impedance (Rs = 1.18 Ω, Rct = 0.84 Ω).
In Vitro Corrosion Behavior of Biodegradable Iron Foams with Polymeric Coating
Authors: Gorejová Radka , Oriňaková Renáta , Orságová Králová Zuzana , Baláž Matej , Kupková Miriam , Hrubovčáková Monika , Haverová Lucia , Džupon Miroslav , Oriňak Andrej , Kaľavský František , Kovaľ Karol
Published: January 2020

Research in the field of biodegradable metallic scaffolds has advanced during the last decades. Resorbable implants based on iron have become an attractive alternative to the temporary devices made of inert metals. Overcoming an insufficient corrosion rate of pure iron, though, still remains a problem. In our work, we have prepared iron foams and coated them with three different concentrations of polyethyleneimine (PEI) to increase their corrosion rates. Scanning electron microscopy (SEM) coupled with energy dispersive X-ray analysis (EDX), Fourier-transform infrared spectroscopy (FT-IR), and Raman spectroscopy were used for characterization of the polymer coating. The corrosion behavior of the powder-metallurgically prepared samples was evaluated electrochemically using an anodic polarization method. A 12 weeks long in vitro degradation study in Hanks’ solution at 37 °C was also performed. Surface morphology, corrosion behavior, and degradation rates of the open-cell foams were studied and discussed. The use of PEI coating led to an increase in the corrosion rates of the cellular material. The sample with the highest concentration of PEI film showed the most rapid corrosion in the environment of simulated body fluids.
Influence of Recycled High-Performance Aggregate on Deformation and Load-Carrying Capacity of Reinforced Concrete Beams
Authors: Sadowska-Buraczewska Barbara , Barnat-Hunek Danuta , Szafraniec Małgorzata
Published: January 2020

The use of recycled concrete aggregates (RCA) in high performance concrete (HPC) was analyzed. The paper presents the experimental studies of model reinforced concrete beams with a rectangular section using high-performance recycled aggregates. Two variable contents of recycled aggregate concrete were used in this study: 50% and 100%. The experimental analyses conducted as immediate studies concerned the following issues: short time loads-deflection, load-carrying capacity of beams, deformation of concrete, cracks, and long-term loads-deflection. The comparative analysis involves the behavior of beams made of high performance concrete-high strength concrete (HPC-HSC) recycled aggregates with model control elements made of regular concrete based on natural aggregates. The deflection values for the recycled aggregate beams were 20% higher than in the case of the control beams made of HPC-HSC exclusively. Replacement of aggregate with recycled concrete aggregate resulted in a large decrease in the value of these two parameters, i.e., compression strength by about 42% and modulus of elasticity by about 33%.
Optimizing Gradation Design for Ultra-Thin Wearing Course Asphalt
Authors: Cui Wentian , Wu Kuanghuai , Cai Xu , Tang Haizhu , Huang Wenke
Published: January 2020

In recent years, ultra-thin wearing course asphalt mixture has been widely used in the reconstruction of old road surfaces and the functional layer of new road surfaces due to its good road performance. To improve the rutting resistance of ultra-thin wearing course asphalt mixture, this research presents an Ultra-thin Wearing Course-10 (UTWC-10) asphalt mixture with good high-temperature stability and skid resistance based on the Taylor system standard mesh specifications. The Course Aggregate Void Filling (CAVF) method is used to design the UTWC-10 asphalt mixture, which is compared with two other traditional ultra-thin wearing course asphalt mixtures on the basis of different laboratory performance tests. The high-temperature rutting test data shows that the rutting dynamic stability (DS) index of the UTWC-10 asphalt mixture is much higher than that of traditional wearing course asphalt mixtures, as it has better high-temperature stability. Moreover, anti-sliding performance attenuation tests are conducted by a coarse aggregate polishing machine. The wear test results show that the skid resistance of the UTWC-10 asphalt mixture is promising. The anti-sliding performance attenuation test can effectively reflect the skid resistance attenuation trend of asphalt pavement at the long-term vehicle load. It is verified that the designed UTWC-10 asphalt mixture shows excellent high-temperature rutting resistance and skid resistance, as well as better low temperature crack resistance and water stability than the traditional wearing course asphalt mixtures.
Insight into the Effect of TDMs on the Tribological Behaviors of the Ionic Liquid Composite Films
Authors: Qi Ya’e , Zhang Ling , Wang Yongxia
Published: January 2020

Ionic liquid (IL) combined with 2D materials has evoked considerable attention in the field of lubrication applications because of their speical structure and outstanding lubrication properties. However, the ambiguous effect of the 2D materials on the friction and anti-wear properties of the IL needs futher study. Here, we have obtained two families of IL composite films with additives of MoS 2 and graphene via a combined process of spin-coated and curing, and the distinction of the effects of two additives on the tribological performance of the IL films was studied. The friction tests showed that the friction coefficient and anti-wear life of the IL films were greatly enhanced after the addition of MoS 2 or graphene, which could be attributed to the improved load-carrying capacity and the second lubrication phase. Under a low addition content, graphene had more advantages to reduce the friction of the films, and MoS 2 was more beneficial to the tribological properties with the additional content increased. The films with low friction and good anti-wear properties may be valuable for the rational design of lubrication films for the practical engineering applications.
Enhanced Magnetoelectric Coupling in BaTiO3-BiFeO3 Multilayers—An Interface Effect
Authors: Hohenberger Stefan , Jochum Johanna K. , Van Bael Margriet J. , Temst Kristiaan , Patzig Christian , Höche Thomas , Grundmann Marius , Lorenz Michael
Published: January 2020

Combining various (multi-)ferroic materials into heterostructures is a promising route to enhance their inherent properties, such as the magnetoelectric coupling in BiFeO3 thin films. We have previously reported on the up-to-tenfold increase of the magnetoelectric voltage coefficient α ME in BaTiO3-BiFeO3 multilayers relative to BiFeO3 single layers. Unraveling the origin and mechanism of this enhanced effect is a prerequisite to designing new materials for the application of magnetoelectric devices. By careful variations in the multilayer design we now present an evaluation of the influences of the BaTiO3-BiFeO3 thickness ratio, oxygen pressure during deposition, and double layer thickness. Our findings suggest an interface driven effect at the core of the magnetoelectric coupling effect in our multilayers superimposed on the inherent magnetoelectric coupling of BiFeO3 thin films, which leads to a giant α ME coefficient of 480 Vc m − 1 Oe − 1 for a 16 × (BaTiO3-BiFeO3) superlattice with a 4.8 nm double layer periodicity.
Effect of Process Orientation on the Mechanical Behavior and Piezoelectricity of Electroactive Paper
Authors: Yoon Sean , Kim Jung Woong , Kim Hyun Chan , Kim Jaehwan
Published: January 2020

This paper reports the effect of process orientation on the mechanical behavior and piezoelectricity of electroactive paper (EAPap) made from natural cotton pulp. EAPap is fabricated by a casting and wet drawing of cellulose film after dissolving cotton with LiCl and DMAc solvent. During the fabrication, permanent wrinkles, a possible factor for performance deterioration, were found in the films. Finite element method was introduced to identify the formation mechanism behind the wrinkles. The simulation results show that the wrinkles were caused by buckling and are inevitable under any conditions. The tensile and piezoelectric tests show that the orientation dependency of the stretched EAPap gives the anisotropic characteristics on both mechanical and piezoelectric properties. In this research, the anisotropic elastic moduli and Poisson’s ratios are reported. The piezoelectric charge constant of EAPap in the linear elastic is calculated. The piezoelectric charge constants of EAPap are associated with the alignment angle in the order of 45° > 0° > 90° due to the strong shear effect. The higher stretching ratio gives the higher piezoelectricity due to the alignment of the molecular chains and the microstructure in EAPap. The highest piezoelectric charge constant is found to be 12 pC/N at a stretching ratio of 1.6 and aligning angle of 45°.