bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Synthesis and Anticancer Potential of 1,4-Dihydropyridines-Based Triazole Derivatives: In Silico and In Vitro Study
Authors: Bijani Sabera , Iqbal Danish , Mirza Sheefa , Jain Vicky , Jahan Sadaf , Alsaweed Mohammed , Madkhali Yahya , Alsagaby Suliman A. , Banawas Saeed , Algarni Abdulrahman , Alrumaihi Faris , Rawal Rakesh M. , Alturaiki Wael , Shah Anamik
Published: April 2022

A library of 1,4-dihydropyridine-based 1,2,3-triazol derivatives has been designed, synthesized, and evaluated their cytotoxic potential on colorectal adenocarcinoma (Caco-2) cell lines. All compounds were characterized and identified based on their 1H and 13C NMR (Nuclear Magnetic Resonance) spectroscopic data. Furthermore, molecular docking of best anticancer hits with target proteins (protein kinase CK2α, tankyrase1, and tankyrase2) has been performed. Our results implicated that most of these compounds have significant antiproliferative activity with IC50 values between 0.63 ± 0.05 and 5.68 ± 0.14 µM. Moreover, the mechanism of action of most active compounds 13ab′ and 13ad′ suggested that they induce cell death through apoptosis in the late apoptotic phase as well as dead phase, and they could promote cell cycle arrest at the G2/M phase. Furthermore, the molecular docking study illustrated that 13ad′ possesses better binding interaction with the catalytic residues of target proteins involved in cell proliferation and antiapoptotic pathways. Based on our in vitro and in silico study, 13ad′ was found to be a highly effective anti-cancerous compound. The present data indicate that dihydropyridine-linked 1,2,3-triazole conjugates can be generated as potent anticancer agents.
Novel Coronavirus (SARS-CoV-2) in Water and Environment—A Scoping Review
Authors: Warsi Taufique , Arora Tanvi , Rizvi Syed Shams , Moosvi Ali Raza , Aslam M. A. Mohammed , Khan Mohammad Muqtada Ali , Mohammed Arifullah
Published: April 2022

A pneumonia outbreak was primarily reported in the fall of 2019 in Wuhan, Hubei province, China, with the identity SARS-CoV-2, a novel coronavirus. It quickly grew from a local epidemic to a global pandemic and was declared a public health emergency by the WHO. A total of three prominent waves were identified across the globe, with a slight temporal variability as per the geographical locations, and has impacted several sectors which connect the world. By March 2022, the coronavirus had infected 444.12 million people and claimed 6.01 million human lives worldwide, and these numbers have not yet stabilized. Our paper enlightens readers on the seven strains of human coronaviruses, with special emphasis on the three severe deadliest outbreaks (SARS-2002, MERS-2012, and COVID-19). This work attempts a comprehensive understanding of the coronavirus and its impact on the possible sectors that link the world through the economic chain, climate conditions, SDGs, recycling of the event, and mitigations. There are many points that are raised by the authors in the possible sectors, which are emerging or are as yet unnoticed and thus have not been taken into consideration. This comprehension will leave sets of new challenges and opportunities for the researchers in various streams, especially in earth sciences. Science-integrated research may help to prevent upcoming disasters as a by-product of (existing) epidemics in the form of coronavirus.
Comparative Study of Rheological Effects of Vegetable Oil-Lubricant, TiO2, MWCNTs Nano-Lubricants, and Machining Parameters’ Influence on Cutting Force for Sustainable Metal Cutting Process
Authors: Okokpujie Imhade P. , Tartibu Lagouge K. , Sinebe Jude E. , Adeoye Adeyinka O. M. , Akinlabi Esther T.
Published: April 2022

Nano-lubricant machining of Aluminum 8112 alloy is the art of sustainable manufacturing of mechanical components used for defense technology and aerospace application. However, machining aluminum alloys generates excess heat, which tends to increase the cutting force (F.C.), due to the material adhesion of the workpiece on the cutting tool. The challenge has drawn researchers’ attention to introducing nano-lubrication processes. This study focused on the comparative assessment of eco-friendly vegetable oil-based-TiO2 and MWCNTs nano-lubricant on cutting force during the machining of the Aluminum 8112 alloy. Nanoparticles were implemented on the base oil using an ultrasonic vibrator and magnetic stirrer before the application in the machining, via the minimum quantity lubrication process. Quadratic central composite designs were employed to carry out the experiment, using five factors at five levels, having experimental runs of 50. The input parameters are helix angle (H.A.), spindle speed (S.S.), axial depth of cut (ADOC), feed rate (F.R.), and length of cut (LOC). The results show that the application of the nanoparticle increases the performance of the vegetable oil on the cutting force. TiO2 nano-lubricant reduces the cutting force by 0.26%, compared with the MWCNTs, and 6% compared with the vegetable oil. Furthermore, the MWCNT nano-lubricant reduces the cutting force by 5% compared with the vegetable oil lubrication environment.
Features of PABIGnx 3D Polymer Gel as an Ionising Radiation Dosimeter
Authors: Kozicki Marek , Jaszczak Malwina , Maras Piotr
Published: April 2022

This work presents the features of the PABIGnx 3D polymer gel dosimeter. It consists of two cross-linkers: poly(ethylene glycol) diacrylate (PEGDA), as one biacrylic component, and N,N′-methylenebisacrylamide (MBA), which is another cross-linker often used in 3D dosimeters. Additionally, it contains oxygen scavenges of copper sulfate pentahydrate and ascorbic acid. All ingredients are embedded in a physical gel matrix of gelatine. Upon irradiation, the biacrylic cross-linking agents (PEGDA and MBA) undergo radical polymerisation and cross-linking, which is manifested by the appearance of the opacity of the intensity related to the absorbed dose. PABIGnx was irradiated with an oncological source of ionising radiation, and analysed by using a nuclear magnetic resonance (0.5 T). The following characteristics were obtained: (i) linear and dynamic dose-response of 0.5 to ~18 Gy and 40 Gy, respectively, (ii) dose sensitivity of 0.071 ± 0.001 Gy−1 s−1, (iii) integral 3D dose distribution for at least 24 days after irradiation, (iv) adequate batch-to-batch reproducibility, (v) dose-response independent of irradiation with 6 MV photons, 15 MV photons, 6 MV photons FFF of 0.0168–0.1094 Gy/s dose rates, and (vi) soft tissue equivalence. The study showed that the features of PABIGnx confirm its suitability for use in 3D radiotherapy dosimetry.
Stability of Spin-Wave Solitons in Bose-Einstein Condensates of Magnons: A Possible Application in Ferromagnetic Films
Authors: Pereira Lucas Carvalho , do Nascimento Valter Aragão
Published: April 2022

In this paper, we theoretically investigate the stability of spin-wave solitons in Bose-Einstein condensates of repulsive magnons, confined by an inhomogeneous external magnetic field described by a Gaussian well. For this purpose, we use the quasi-one-dimensional Gross-Pitaevskii equation to describe the behavior of the condensate. In order to solve the Gross-Pitaevskii equation, we used two different approaches: one analytical (variational method) and another numerical (split-step Crank-Nicolson method). The stability of the solutions and the validation of the numerical results were confirmed, respectively, through the anti-VK criterion and the virial theorem. Furthermore, the simulations described the behavior of physical quantities of interest such as chemical potential, energy per magnon and central density as a function of the nonlinearity of the model (magnon-magnon interactions). The theoretical results provide subsidies for a better understanding of the nonlinear phenomena related to the Bose-Einstein condensates of magnons in ferromagnetic films.
Diamond Structures for Tuning of the Finesse Coefficient of Photonic Devices
Authors: Kosowska Monika , Mallik Awadesh K. , Rycewicz Michał , Haenen Ken , Szczerska Małgorzata
Published: April 2022

Finesse coefficient is one of the most important parameters describing the properties of a resonant cavity. In this research, a mathematical investigation of the application of diamond structures in a fiber-optic Fabry–Perot measurement head to assess their impact on the finesse coefficient is proposed. We present modeled transmission functions of cavities utilizing a nitrogen-doped diamond, a boron-doped diamond, nanocrystalline diamond sheet and a silver mirror. The diamond structures were deposited using a microwave plasma-assisted chemical vapor deposition system. A SEM investigation of surface morphology was conducted. The modeling took into consideration the fiber-optic Fabry–Perot setup working in a reflective mode, with an external cavity and a light source of 1550 nm. A comparison of the mathematical investigation and experimental results is presented.
Evaluation of Antimicrobial Efficacy of UVC Radiation, Gaseous Ozone, and Liquid Chemicals Used for Disinfection of Silicone Dental Impression Materials
Authors: Wezgowiec Joanna , Wieczynska Anna , Wieckiewicz Mieszko , Czarny Anna , Malysa Andrzej , Seweryn Piotr , Zietek Marek , Paradowska-Stolarz Anna
Published: April 2022

Effective disinfection of dental impressions is an indispensable requirement for the safety of dental personnel and patients. The ideal method should be not only effective but also convenient, cheap, and environmentally friendly. This study aimed to reliably evaluate the efficacy of ultraviolet C (UVC) radiation, gaseous ozone, and commercial liquid chemicals used for silicone dental impressions disinfection. These methods were applied to two types of elastomeric impression materials: condensation silicones and addition silicones of various consistency (putty, medium, and light). The antimicrobial effectiveness against Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans was evaluated in vitro by counting colony-forming units (CFU) on the surface of samples. The one-way ANOVA with a Tukey HSD test or the Kruskal–Wallis with a Dunn’s test was performed. The results obtained revealed the efficacy of the proposed methods for disinfection of both C-silicones and A-silicones in most of the studied groups. Only one material (Panasil initial contact Light) was not effectively disinfected after UVC irradiation or ozone application. In conclusion, the potential of each disinfection method should be evaluated separately for each material. Moreover, in further research, the possible influence of the proposed methods on the physical properties of the impression materials should be thoroughly investigated.
The Effect of Mask Style and Fabric Selection on the Comfort Properties of Fabric Masks
Authors: Gericke Adine , Militký Jiří , Venkataraman Mohanapriya , Steyn Hester , Vermaas Jana
Published: April 2022

The purpose of fabric masks in the prevention of the spread of COVID-19 often requires that the masks be worn for extended periods without removal. The management of the conditions in the micro-climate inside the masks is important to keep the wearer comfortable and enhance user compliance. In this study, the effect of mask design and fabric type on the micro-climate was investigated using thermocron iButtons to record the temperature and humidity inside the masks. It was found that the mask style, and its effect on the amount of air incorporated in the micro-climate, had a significant influence on the factors that determine the temperature and humidity levels. In the shaped masks, the impact of the mask design on the results was stronger than the effect of fabric type. In the folded masks that fit snugly around the face, the effect of fabric type was significant, and both fibre composition and fabric structure contributed to the differences in the performance of the three fabrics tested. In the case of the masks with an inserted filter, a significant amount of trapped still air in the fabric layers and the increased mask stiffness had the strongest effect on the temperature and humidity inside the masks. Significant differences were also found in the temperatures recorded in the different time segments, highlighting the importance of conducting comfort evaluations over a long enough time to prevent false interpretations. The results of this study emphasize the importance of considering all the components of mask design, namely style, fibre type, and fabric structure, in the development of masks to enhance user compliance.
Effects of Temperature and Applied Potential on the Stress Corrosion Cracking of X80 Steel in a Xinzhou Simulated Soil Solution
Authors: Cheng Yuan , Liu Peng , Yang Mengmeng
Published: April 2022

In this research, the stress corrosion cracking (SCC) behavior of X80 pipeline steel in a Xinzhou soil environment at different temperatures and applied potentials was studied with a slow strain rate test (SSRT), potentiodynamic polarization curve measurements, and scanning electron microscopy (SEM). When a higher anodic potential was applied, anodic dissolution occurred at the crack tip and on the crack wall. The cracking mechanism of X80 steel in Xinzhou soil solution is anodic dissolution (AD). At positive cathodic potentials, X80 steel is under an anodic polarization state at the crack tip and under a cathodic polarization state at the crack wall. The SCC of X80 steel is affected by the combined effects of anodic dissolution (AD) and hydrogen embrittlement (HE). At more negative cathodic potentials, both crack tips and crack walls are under cathodic polarization. The SCC of X80 steel is dominated by hydrogen embrittlement (HE). SCC susceptibility has the same variation trend with potentials at different temperatures. The susceptibility to SCC increases notably as the temperature increases at weak cathodic potentials and open circuit potential due to the effect of temperature on the corrosion potential and the diffusion of atoms.
Hemp and Its Derivatives as a Universal Industrial Raw Material (with Particular Emphasis on the Polymer Industry)—A Review
Authors: Tutek Karol , Masek Anna
Published: April 2022

This review article provides basic information about cannabis, its structure, and its impact on human development at the turn of the century. It also contains a brief description of the cultivation and application of these plants in the basic branches of the economy. This overview is also a comprehensive collection of information on the chemical composition of individual cannabis derivatives. It contains the characteristics of the chemical composition as well as the physicochemical and mechanical properties of hemp fibers, oil, extracts and wax, which is unique compared to other review articles. As one of the few articles, it approaches the topic in a holistic and evolutionary way, moving through the plant’s life cycle. Its important element is examples of the use of hemp derivatives in polymer composites based on thermoplastics, elastomers and duroplasts and the influence of these additives on their properties, which cannot be found in other review articles on this subject. It indicates possible directions for further technological development, with particular emphasis on the pro-ecological aspects of these plants. It indicates the gaps and possible research directions in basic knowledge on the use of hemp in elastomers.
Fibroblasts Mediate Ectopic Bone Formation of Calcium Phosphate Ceramics
Authors: Fu Liangliang , Zhao Qin , Li Jiaojiao , Zhao Zifan , Wang Min , Sun Huifang , Xia Haibin
Published: April 2022

Heterogeneity of fibroblasts directly affects the outcome of tissue regeneration; however, whether bioactive ceramics regulate bone regeneration through fibroblasts is unclear. Ectopic bone formation model with biphasic calcium phosphate (BCP) implantation was used to investigate the temporal and spatial distribution of fibroblasts around ceramics. The effect of BCP on L929 fibroblasts was evaluated by EdU assay, transwell assay, and qRT-PCR. Further, the effect of its conditioned medium on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was confirmed by ALP staining. SEM and XRD results showed that BCP contained abundant micro- and macro-pores and consisted of hydrogen-apatite (HA) and β-tricalcium phosphate (β-TCP) phases. Subsequently, BCP implanted into mice muscle successfully induced osteoblasts and bone formation. Fibroblasts labelled by vimentin gathered around BCP at 7 days and peaked at 14 days post implantation. In vitro, BCP inhibited proliferation of L929 fibroblast but promoted its migration. Moreover, expression of Col1a1, Bmp2, and Igf1 in L929 treated by BCP increased significantly while expression of Tgfb1 and Acta did not change. ALP staining further showed conditioned media from L929 fibroblasts treated by BCP could enhance osteogenic differentiation of BMSCs. In conclusion, fibroblasts mediate ectopic bone formation of calcium phosphate ceramics.
Total Knee Replacement with an Uncemented Porous Tantalum Tibia Component: A Failure Analysis
Authors: Fokter Samo K. , Gubeljak Nenad , Punzón-Quijorna Esther , Pelicon Primož , Kelemen Mitja , Vavpetič Primož , Predan Jožef , Ferlič Luka , Novak Igor
Published: April 2022

Porous tantalum has been extensively used in orthopaedic surgery, including uncemented total knee arthroplasty (TKA). Favourable results were reported with earlier monobloc tibial components and the design evolved to modular implants. We aimed to analyse possible causes for extensive medial tibia bone loss, resulting in modular porous tantalum tibia baseplate fracture after primary TKA. Retrieved tissue samples were scanned with 3 MeV focused proton beam for Proton-Induced X-ray Emission (micro-PIXE) elemental analysis. Fractographic and microstructural analysis were performed by stereomicroscopy. A full 3D finite-element model was made for numerical analysis of stress–strain conditions of the tibial baseplate. Histological examination of tissue underneath the broken part of the tibial baseplate revealed dark-stained metal debris, which was confirmed by micro-PIXE to consist of tantalum and titanium. Fractographic analysis and tensile testing showed that the failure of the tibial baseplate fulfilled the criteria of a typical fatigue fracture. Microstructural analysis of the contact surface revealed signs of bone ingrowth in 22.5% of the surface only and was even less pronounced in the medial half of the tibial baseplate. Further studies are needed to confirm the responsibility of metal debris for an increased bone absorption leading to catastrophic tibial tray failure.
Comparative Study of Effects of Air-Entraining Plasticizing Admixture and Lime on Physical and Mechanical Properties of Masonry Mortars and Plasters
Authors: Gołaszewska Małgorzata , Gołaszewski Jacek , Bochen Jerzy , Cygan Grzegorz
Published: April 2022

This article presents research on selected physical and mechanical properties of cement-based plasters and masonry mortars with consistency-improving additives, namely, traditional hydrated lime and a plasticizing and aerating mixture (APA), which, in practice, is often considered to be a lime substitute. Comparative analysis of the properties of mortars with alternative additives—lime or APA—was carried out, taking into consideration possible effects of cement, as two types of Portland cement were used for the research. For fresh mortar, mixture consistency, air content, resistance to segregation, and water retention were determined. Tests on hardened mortars included tests of porosity and impermeability, depth of penetration of water under pressure, drying shrinkage, as well as compressive and bending strength, modulus of elasticity, and adhesion of mortars to the base. In addition, research has shown that cement–lime mortars and cement mortars with APA admixture of similar consistency in the fresh state are characterized by significantly different properties. The results show, in most of the features analyzed, more favorable properties of mortars with the use of traditional lime. For shrinkage only, the use of admixture turned out to be more advantageous.
Improving the Shape Memory Effect of a Fe-Mn-Si-Cr-Ni Alloy through Shot Peening
Authors: Yang Huanping , Yan Wenbin , Deng Xuyang , Zhang Mengqi , Wang Yaomian
Published: April 2022

To improve the shape memory effect, the solutionized Fe-24Mn-6Si-9Cr-6Ni alloy was shot peened and subsequently annealed. The phase constituent was examined using the X-ray diffraction method. Microstructure evolution was characterized using an optical microscope and the electronic backscatter diffraction method, and the shape memory effect was evaluated using a bending test. The results show that α′-martensite and ε-martensite were introduced into the shot-peened surface layer. The α′-martensite remained after annealing even at 850 °C. Microstructure of the surface layer was refined through shot peening and subsequent annealing. Compared with those of the solutionized specimen, the shape recovery ratio and recovery strain of the specimens that are shot peened and subsequently annealed are significantly improved at different prestrains.
CFD Analysis of Wind Distribution around Buildings in Low-Density Urban Community
Authors: Hnaien Nidhal , Hassen Walid , Kolsi Lioua , Mesloub Abdelhakim , Alghaseb Mohammed A. , Elkhayat Khaled , Abdelhafez Mohamed Hssan Hassan
Published: April 2022

The computational fluid dynamics (CFDs) models based on the steady Reynolds-averaged Navier–Stokes equations (RANSs) using the k−ω two-equation turbulence model are considered in order to estimate the wind flow distribution around buildings. The present investigation developed a micro-scale city model with building details for the Hail area (Saudi Arabia) using ANSYS FLUENT software. Based on data from the region’s meteorological stations, the effect of wind speed (from 2 to 8 m/s) and wind direction (north, east, west, and south) was simulated. This study allows us to identify areas without wind comfort such as the corner of the building and the zones between adjacent buildings, which make this zone not recommended for placement of restaurants, pedestrian passages, or gardens. Particular attention was also paid to the highest building (Hail Tower, 67 m) in order to estimate, along the tower height, the wind speed effect on the turbulence intensity, the turbulent kinetic energy (TKE), the friction coefficient, and the dynamic pressure.
Prophesying the Short-Term Dynamics of the Crude Oil Future Price by Adopting the Survival of the Fittest Principle of Improved Grey Optimization and Extreme Learning Machine
Authors: Das Asit Kumar , Mishra Debahuti , Das Kaberi , Mallick Pradeep Kumar , Kumar Sachin , Zymbler Mikhail , El-Sayed Hesham
Published: April 2022

Crude oil market analysis has become one of the emerging financial markets and the volatility effect of the market is paramount and has been considered as an issue of utmost importance. This study examines the dynamics of this volatile market of crude oil by employing a hybrid approach based on an extreme learning machine (ELM) as a regressor and the improved grey wolf optimizer (IGWO) for prophesying the crude oil rate for West Texas Intermediate (WTI) and Brent crude oil datasets. The datasets are augmented using technical indicators (TIs) and statistical measures (SMs) to obtain better insight into the forecasting ability of this proposed model. The differential evolution (DE) strategy has been used for evolution and the survival of the fittest (SOF) principle has been used for elimination while implementing the GWO to achieve better convergence rate and accuracy. Whereas, the algorithmic simplicity, use of less parameters, and easy implementation of DE efficiently decide the evolutionary patterns of wolves in GWO and the SOF principle updates the wolf pack based on the fitness value of each wolf, thereby ensuring the algorithm does not fall into local optimum. Furthermore, the comparison and analysis of the proposed model with other models, such as ELM–DE, ELM–Particle Swarm Optimization (ELM–PSO), and ELM–GWO shows that the predictability evidence obtained substantially achieves better performance for ELM–IGWO with respect to faster error convergence rate and mean square error (MSE) during training and testing phases. The sensitivity study of the proposed ELM–IGWO provides better results in terms of the performance measures, such as Theil’s U, mean absolute error (MAE), average relative variance (ARV), mean average percentage error (MAPE), and minimal computational time.
Exploring the Diversity of Red Microalgae for Exopolysaccharide Production
Authors: Borjas Esqueda Aldo , Gardarin Christine , Laroche Céline
Published: April 2022

Microalgae constitute a remarkable biological diversity but a limited number of them have been the object of study for their ability to produce exoplysaccharides (EPS). Among them, the red marine microalgae Porphyridium or Rhodella produce sulphated EPS, exhibiting some biological activities with potential interest in the pharmaceutical and cosmetic industries. EPS from Porphyridium and Rhodella being relatively similar in their composition, it has long been considered that all the red microalgae produced similar EPS and no attention was paid to other red microalgae. The objective of our work was then to explore the diversity of red microalgae for the production of EPS, focusing in this first step on the screening of the strains for their ability to produce EPS and preliminary structural characterization. The study was conducted with 11 microalgae strains belonging to the proteorhodophytina subphylum. All microalgae were able to produce EPS, released in the culture medium (strains belonging to Porphyridiophyceae and Rhodellophyceae classes) or remaining bound to the cells (strains from Stylonematophyceae class). The analysis of monosaccharides composition was found significantly different, with for instance high levels of glucuronic acids in the EPS from C. japonica and N. cyanea, but also strong differences in the sulphation degrees of polymers (between 1.2 and 28.7% eq. SO4).
Pan-Genomic and Transcriptomic Analyses of Marine Pseudoalteromonas agarivorans Hao 2018 Revealed Its Genomic and Metabolic Features
Authors: Jv Yuhao , Xi Chenxiang , Zhao Yanqiu , Wang Wei , Zhang Yiling , Liu Kai , Liu Wenlin , Shan Kai , Wang Chunlei , Cao Ruiwen , Dai Cunxi , Jv Yiting , Zhu Wenxing , Wang Haiyong , He Qiuxia , Hao Lujiang
Published: April 2022

The genomic and carbohydrate metabolic features of Pseudoalteromonas agarivorans Hao 2018 (P. agarivorans Hao 2018) were investigated through pan-genomic and transcriptomic analyses, and key enzyme genes that may encode the process involved in its extracellular polysaccharide synthesis were screened. The pan-genome of the P. agarivorans strains consists of a core-genome containing 2331 genes, an accessory-genome containing 956 genes, and a unique-genome containing 1519 genes. Clusters of Orthologous Groups analyses showed that P. agarivorans harbors strain-specifically diverse metabolisms, probably representing high evolutionary genome changes. The Kyoto Encyclopedia of Genes and Genomes and reconstructed carbohydrate metabolic pathways displayed that P. agarivorans strains can utilize a variety of carbohydrates, such as d-glucose, d-fructose, and d-lactose. Analyses of differentially expressed genes showed that compared with the stationary phase (24 h), strain P. agarivorans Hao 2018 had upregulated expression of genes related to the synthesis of extracellular polysaccharides in the logarithmic growth phase (2 h), and that the expression of these genes affected extracellular polysaccharide transport, nucleotide sugar synthesis, and glycosyltransferase synthesis. This is the first investigation of the genomic and metabolic features of P. agarivorans through pan-genomic and transcriptomic analyses, and these intriguing discoveries provide the possibility to produce novel marine drug lead compounds with high biological activity.
Prevalence of Cigarette Smoking and Influence of Associated Factors among Students of the University of Banja Luka: A Cross-Sectional Study
Authors: Todorović Ivana , Cheng Feng , Stojisavljević Stela , Marinković Sonja , Kremenović Stefan , Savić Pane , Golić-Jelić Ana , Stojaković Nataša , Stoisavljević-Šatara Svjetlana , Igić Rajko , Škrbić Ranko
Published: April 2022

Background and Objectives: Cigarette smoking among the youth population has increased significantly in developing countries, including Bosnia and Herzegovina. However, no extant literature assesses the prevalence of tobacco use, nor identifies factors associated with smoking. This study determined the prevalence of cigarette smoking among a specific cohort of students and assessed factors related to tobacco use in this population. Materials and Methods: This cross-sectional study included 1200 students at all faculties of Banja Luka University. Data were collected from questionnaires adapted from the Global Youth Tobacco Survey (GYTS) and the Global Health Professional Student Survey (GHPSS) standardized questionnaires and were analyzed using descriptive statistics, Pearson’s χ2 test, and logistic regression. Results: When the prevalence of cigarette smoking within the last thirty days was recorded, we found that 34.1% of students smoked within this period. Nearly three-quarters (74.9%) of the student population had smoked or experimented with cigarette smoking. However, medical students were 27.2% less likely to smoke than their counterparts from other faculties. Overall, 87% of all students were aware of the harmful effects of cigarette smoking, 79% were aware of the harmful effects of secondhand smoke, and 65% reported that it was difficult to quit. Increased spending of personal money was associated with a higher probability of smoking, while exposure to secondhand smoke increased the odds of smoking by 62%. Conclusion: Policies, strategies, and action plans should be introduced in order to reduce the prevalence of smoking among university students and to create a smoke-free environment at the various universities involved.
A Novel Hybrid Reactor of Pressure-Retarded Osmosis Coupling with Activated Sludge Process for Simultaneously Treating Concentrated Seawater Brine and Wastewater and Recovering Energy
Authors: Ding Jiaxin , Zhou Qian , Zhou Zixun , Chu Wenyu , Jiang Yao , Lai Wei , Zhao Pin , Wang Xinhua
Published: April 2022

As an attractive way to deal with fresh water shortage, membrane-based desalination technologies are receiving increased interest. However, concentrated seawater brine, in needing further treatment, remains a main obstacle for desalination via membrane technology. Here, a hybrid technology integrating pressure-retarded osmosis with activated sludge process (PRO-MBR) was applied for simultaneously treating concentrated seawater brine and municipal wastewater. Performance of the PRO-MBR, including water flux, power density, contaminants removal, and membrane fouling was evaluated and compared at two different membrane orientations (i.e., active layer facing feed solution (AL-FS) mode and active layer facing draw solution (AL-DS) mode). During the PRO-MBR process, the municipal wastewater was completely treated regardless of the membrane orientation, which means that there was no concentrated sewage needing further treatment, owing to the biodegradation of microorganisms in the bioreactor. In the meantime, the concentrated brine of seawater desalination was diluted into the salinity level of seawater, which met the standard of seawater discharge. Owing to the high rejection of forward osmosis (FO) membrane, the removal efficiency of total organic carbon (TOC), total phosphorus (TP), ammonia nitrogen (NH4+-N), and total nitrogen (TN) was higher than 90% at both modes in the PRO-MBR. In addition, the PRO-MBR can simultaneously recover the existing osmotic energy between the municipal wastewater and the seawater brine at both modes. Compared with the AL-DS mode, the AL-FS mode took a shorter time and achieved a bigger power density to reach the same terminal point of the PRO-MBR owing to a better water flux performance. Furthermore, the membrane fouling was much more severe in the AL-DS mode. In conclusion, the current study demonstrated that the PRO-MBR at the AL-FS mode can be a promising and sustainable brine concentrate and municipal wastewater treatment technology for its simultaneous energy and water recovery.
Dynamic Distribution of ASIC1a Channels and Other Proteins within Cells Detected through Fractionation
Authors: Salinas Castellanos Libia Catalina , Gatto Rodolfo Gabriel , Menchón Silvia Adriana , Blaustein Matías , Uchitel Osvaldo Daniel , Weissmann Carina
Published: April 2022

Proteins in eukaryotic cells reside in different cell compartments. Many studies require the specific localization of proteins and the detection of any dynamic changes in intracellular protein distribution. There are several methods available for this purpose that rely on the fractionation of the different cell compartments. Fractionation protocols have evolved since the first use of a centrifuge to isolate organelles. In this study, we described a simple method that involves the use of a tabletop centrifuge and different detergents to obtain cell fractions enriched in cytosolic (Cyt), plasma membrane (PM), membranous organelle (MO), and nuclear (Nu) proteins and identify the proteins in each fraction. This method serves to identify transmembrane proteins such as channel subunits as well as PM-embedded or weakly associated proteins. This protocol uses a minute amount of cell material and typical equipment present in laboratories, and it takes approximately 3 h. The process was validated using endogenous and exogenous proteins expressed in the HEK293T cell line that were targeted to each compartment. Using a specific stimulus as a trigger, we showed and quantified the shuttling of a protein channel (ASIC1a, acid sensing ion channel) from the MO fraction to the PM fraction and the shuttling of a kinase from a cytosolic location to a nuclear location.
In Vitro Corrosion Performance of As-Extruded Mg–Gd–Dy–Zr Alloys for Potential Orthopedic Applications
Authors: Liu Chen , Wang Junsheng , Xu Yongdong , Fu Yu , Han Jungang , Cao Zhaoxun , Chen Xiaohu , Zhao Shuming , Zhu Xiurong
Published: April 2022

In this study, different contents of rare earth elements with high solid solubility (Gd and Dy) were added into Mg and fabricated through homogenization and hot extrusion processes that enable few second phase formation to efficaciously inhibit the galvanic corrosion. The microstructure and phase characterization of the as-extruded Mg–Gd–Dy–Zr alloys were analyzed by scanning electron microscopy, electron backscattered diffraction, and X-ray diffraction. The in vitro biodegradation behavior of the as-extruded Mg–Gd–Dy–Zr alloys was investigated via the electrochemical measurement and immersion test. The results revealed that all the as-extruded alloys with different RE additions exerted fully recrystallized microstructures. The average grain size was appropriately 20 μm to 30 μm for all alloys and gradually increased by adding more RE. Only a few tiny second-phase particles less than 5 μm dispersed for all the samples and the volume fraction of particles increased slightly with the increase in RE content. The as-extruded Mg–Gd–Dy–Zr alloys with low RE content (GD0.6) allowed for a satisfactory corrosion resistance in Hank’s solution with a controlled corrosion rate less than 0.5 mm/year, which is considered as the tolerance limit for the corrosion rate of orthopedic implants. This study provides a cost-effective choice for promoting biodegradable magnesium alloys for potential orthopedic applications with low rare earth content in Mg alloys.
A Study of Physical Modeling and Mathematical Modeling on Inclusion Behavior in a Planar Flow Casting Process
Authors: Liu Yu , Qiu Hao , He Zixu , Yu Yue , Liu Heping
Published: April 2022

Flow pattern and inclusion removal in a novel-designed crucible in the planar flow casting process were studied in the present paper. A color dye water experiment was used to show the flow field and validate the mathematical modeling. It was shown that the flow pattern predicted by the Large Eddy Simulation model is maximally consistent with that in the physical modeling. The validated mathematical model was used to predict the flow pattern and inclusion behavior in the crucible with various prototypes using combinations of multiple facilities. The results show that the flow field, by using the stopper, enhances compared to that using none of the facilities, which causes the inclusion removal to become even worse when the stopper is individually used. Furthermore, as the magnetic field is added, the electromagnetic brake weakens the flow field in the magnetic zone. The function of the electromagnetic brake to weaken the flow pattern is most efficient for inclusion removal.
A Genome-Scale Metabolic Model of Methanoperedens nitroreducens: Assessing Bioenergetics and Thermodynamic Feasibility
Authors: He Bingqing , Cai Chen , McCubbin Tim , Muriel Jorge Carrasco , Sonnenschein Nikolaus , Hu Shihu , Yuan Zhiguo , Marcellin Esteban
Published: April 2022

Methane is an abundant low-carbon fuel that provides a valuable energy resource, but it is also a potent greenhouse gas. Therefore, anaerobic oxidation of methane (AOM) is an essential process with central features in controlling the carbon cycle. Candidatus ‘Methanoperedens nitroreducens’ (M. nitroreducens) is a recently discovered methanotrophic archaeon capable of performing AOM via a reverse methanogenesis pathway utilizing nitrate as the terminal electron acceptor. Recently, reverse methanogenic pathways and energy metabolism among anaerobic methane-oxidizing archaea (ANME) have gained significant interest. However, the energetics and the mechanism for electron transport in nitrate-dependent AOM performed by M. nitroreducens is unclear. This paper presents a genome-scale metabolic model of M. nitroreducens, iMN22HE, which contains 813 reactions and 684 metabolites. The model describes its cellular metabolism and can quantitatively predict its growth phenotypes. The essentiality of the cytoplasmic heterodisulfide reductase HdrABC in the reverse methanogenesis pathway is examined by modeling the electron transfer direction and the specific energy-coupling mechanism. Furthermore, based on better understanding electron transport by modeling, a new energy transfer mechanism is suggested. The new mechanism involves reactions capable of driving the endergonic reactions in nitrate-dependent AOM, including the step reactions in reverse canonical methanogenesis and the novel electron-confurcating reaction HdrABC. The genome metabolic model not only provides an in silico tool for understanding the fundamental metabolism of ANME but also helps to better understand the reverse methanogenesis energetics and its thermodynamic feasibility.
Measurement of Conducted Supraharmonic Emissions: Quasi-Peak Detection and Filter Bandwidth
Authors: Frigo Guglielmo
Published: April 2022

In modern power systems, the integration of renewable energy sources relies on dedicated inverters whose power electronic circuitry switches at high frequencies and causes conducted emissions in the supraharmonic range, i.e., from 9 to 150 kHz. In this regard, the normative framework is still lacking a reference measurement method as well as a set of emission limits and performance requirements. From a metrological point of view, it is important to evaluate whether some of the power quality indices adopted for radiated emissions could be transposed also in this context. In particular, the paper considers a recent algorithm for the identification of supraharmonic components and discusses how its estimates affect the estimation of quasi-peak values. To this end, the paper describes the implementation of a fully digital approach and validates the results by means of an experimental comparison against a traditional quasi-peak detector. The proposed analysis confirms the potential of the considered approach and provides some interesting insights about the reliability of quasi-peak estimation in supraharmonic range.
Design and Dynamic Simulation of a Novel Traveling Wave Linear Ultrasonic Motor
Authors: Yang Lin , Yao Kaixi , Ren Weihao , Chen Liang , Yang Mojian , Zhao Rongcheng , Tang Siyu
Published: April 2022

To overcome the problem of frequency consistency and simplify the design process of linear ultrasonic motor, a novel traveling wave linear ultrasonic motor with a ring-type stator is proposed in this paper. The combination of two orthogonal bending vibration modes with the same order is selected as the operating mode of the motor. A traveling wave along the side of the stator is utilized to drive the slider to move linearly. The stator adopts a ring symmetrical structure, which can effectively ensure that the resonance frequencies of the two vibration modes are consistent. Thus, we do not need to tune the frequencies of the two vibrations by constantly adjusting the shape of the stator or designing complex clamping parts to fix the stator without making any influence on the vibrations. Meanwhile, a three-dimensional finite element model of the motor is built. Using the model, we obtain the elliptical motion trajectories of the stator driving surface, the output performance of the motor, the sticking-slipping-separation contact characteristic between the stator and the slider and fabricate and measure a prototype of the proposed motor.
Effect of Pore Shape on Mechanical Properties of Porous Shape Memory Alloy
Authors: Liu Bingfei , Pan Yaxuan
Published: April 2022

Porous shape memory alloys (SMAs) have been widely used in the aerospace, military, medical, and health fields due to its unique mechanical properties such as superelasticity, biocompatibility, and shape memory effect. In this work, the pore shape was considered in the constitutive model of the porous SMAs by respectively introducing the parameter of aspect ratio and for different pore shapes including oblate, sphere, and prolate shapes, so the expression of Young’s modulus for the porous SMA can be derived. Then, the constitutive model for such a porous shape memory alloy was established. When the porosity was zero, the model can be degenerated to the dense case. The stress–strain curves for the porous SMA with a porosity of 13% with different aspect ratio are then given. Numerical results showed good agreement with the published experimental data that proved the validation of the model. Based on the proven constitutive model, the properties of porous SMA with different porosity and pore shapes are discussed. The results showed that the pore shapes and the porosities had a big effect on the stress–strain curves for the porous shape memory, while with the increasing porosities, the Young’s modulus and the hysteresis both decreased. With the same porosities, the Young’s modulus and hysteresis loop of SMA with round pores were the largest, while the Young’s modulus and hysteresis loop were the smallest when r=0.1, and they were greater when r=0.75 than when r=10. It can be seen that the closer to the circle, the better the performance of the material.
Enrichment of Anaerobic Microbial Communities from Midgut and Hindgut of Sun Beetle Larvae (Pachnoda marginata) on Wheat Straw: Effect of Inoculum Preparation
Authors: Schroeder Bruna Grosch , Logroño Washington , Rocha Ulisses Nunes da , Harms Hauke , Nikolausz Marcell
Published: April 2022

The Pachnoda marginata larva have complex gut microbiota capable of the effective conversion of lignocellulosic biomass. Biotechnological utilization of these microorganisms in an engineered system can be achieved by establishing enrichment cultures using a lignocellulosic substrate. We established enrichment cultures from contents of the midgut and hindgut of the beetle larva using wheat straw in an alkaline medium at mesophilic conditions. Two different inoculation preparations were used: procedure 1 (P1) was performed in a sterile bench under oxic conditions using 0.4% inoculum and small gauge needles. Procedure 2 (P2) was carried out under anoxic conditions using more inoculum (4%) and bigger gauge needles. Higher methane production was achieved with P2, while the highest acetic acid concentrations were observed with P1. In the enrichment cultures, the most abundant bacterial families were Dysgonomonadaceae, Heliobacteriaceae, Ruminococcaceae, and Marinilabiliaceae. Further, the most abundant methanogenic genera were Methanobrevibacter, Methanoculleus, and Methanosarcina. Our observations suggest that in samples processed with P1, the volatile fatty acids were not completely converted to methane. This is supported by the finding that enrichment cultures obtained with P2 included acetoclastic methanogens, which might have prevented the accumulation of acetic acid. We conclude that differences in the inoculum preparation may have a major influence on the outcome of enrichment cultures from the P. marginata larvae gut.
Effect of Chemical Corrosion and Axial Compression on the Dynamic Strength Degradation Characteristics of White Sandstone under Cyclic Impact
Authors: Xue Jinchun , Zhao Zhuyu , Dong Longjun , Jin Jiefang , Zhang Yingbin , Tan Li , Cai Ruoyan , Zhang Yihan
Published: April 2022

Both chemical corrosion and axial compression impose critical influences on the internal microstructure of rock. Meanwhile, chemical corrosion can change a rock’s mineral composition, which in turn affects the physical and mechanical properties of the rock. To investigate the dynamic strength characteristics of white sandstone under the coupling effect of axial load and chemical corrosion, a dynamic and static combined loading test device was adopted for performing cyclic impact tests on white sandstone immersed in chemical solution. The results show that with the increasing number of cycles under the same load, the peak strength of the rock presented a trend of ‘strengthening first and then weakening’. The strength of rock resistance to impact failure reached its maximum when the solution of pH was 7 and axial pressure was 12.6 MPa. Under the same axial pressure, the effect of solution pH on the initial dynamic strength of white sandstone is a normal distribution. Acidic and alkaline environments are harmful to rocks during the initial impact, while neutral environments exert little effect and the pH of the solution influences the particle size of impact crushing particles. In addition, the chemical solution has a significant effect on the deterioration of rock strength during the process of initial impact, and the effect is inconspicuous in the later period.