bluuebunny/crossref_metadata_2025 huggingface dataset:
105 Chromium propionate improves insulin sensitivity in horses following oral concentrate consumption
Authors: Spears Jerry W , Siciliano Paul , Pratt-Phillips Shannon , Lloyd Karen , Goertzen Ellen , Krafka Kristi , Hyda Jill , Rounds Whitney
Published: November 2020

Forty-eight Quarter Horse geldings (3–8 yr of age) were used to determine the effects of dietary chromium (Cr), in the form of Cr propionate (Cr Prop), on insulin sensitivity. Horses were blocked by age, body condition score, and glucose response to concentrate feeding on d 0, and randomly assigned to treatments. Treatments consisted of 0, 2, 4, or 8 mg Cr/d from Cr Prop. Horses were fed daily a concentrate mix at a rate of 0.2 kg/100 kg BW and grass hay at 1.75 to 2.0 kg/100 kg BW. After an overnight fast, jugular blood samples were obtained at 0, 2, and 4 h after concentrate feeding on d 0 and 28 for determination of glucose and insulin. Plasma glucose on d 28 was affected (P < 0.05) by treatment, time, and treatment x time. Horses fed 4 mg Cr/d had lesser (P < 0.05) plasma glucose concentrations than those in the other treatments at 0 h. At 2 h post feeding glucose concentrations were greater (P < 0.05) in control horses than in those given 4 mg Cr. Horses fed 2 mg Cr/d had lesser (P < 0.05) plasma glucose at 4 h post feeding compared to controls. Plasma glucose did not differ among horses receiving 2 or 4 mg Cr/d at 2 or 4 h. Insulin concentrations were greater (P < 0.05) in horses fed 0 or 2 mg Cr/d than in those given 4 or 8 mg Cr at 0 h. At 4 h post feeding insulin concentrations were greater (P < 0.05) in control horses than in those fed 2 or 4 mg Cr/d. Results of this study indicate that 2 or 4 mg Cr/d from Cr Prop increased insulin sensitivity in adult horses following oral carbohydrate consumption.
363 Meat Quality Matters: Trends and Mitigation Strategies for Muscle Myopathies in the Broiler Meat Industry
Authors: Owens Casey M , Jackson Ashunti Ria
Published: November 2020

Broiler breast myopathies, including woody breast (WB), white striping (WS), and spaghetti breast (SB), negatively impact the industry. Broiler breast myopathy trends show that myopathies generally increase as the birds get older and larger, and they also increase as breast yield increases to higher levels. Gender can have some impact on myopathies, as males tend to have a higher frequency of WB and females have a higher frequency of SB. Mitigation strategies in both plant and live production are needed. In the short term, it is possible to sort carcasses/fillets manually in processing plants and technology is being developed to detect these issues automatically. These online methods may include image analysis of carcass/fillet shape or dimension features, or evaluating compression force to determine hardness. Fillets determined to be severe for these defects may be diverted into products such as comminuted ones, that are less impacted by appearance or reduced functionality relative to whole fillets. In live production, evaluating strategies to slow down growth rate overall (e.g. management or nutrition), or at strategic times, may be worth investigating, though this would be a short-term mitigation strategy. In the long term, strategies should focus on genetic programs. Breeder companies have always been committed in producing exceptional products. A multitude of methods for early detection of myopathies have tested over the years. Currently, innovative techniques centered around machine learning and more structured breeding programs have been leading the way in further improving meat quality without sacrificing yield. These and other intriguing approaches centralizing on the live bird will drive a positive trend in the quality and quantity of protein produced in the broiler industry.
121 Effects of DON on the porcine growth performance and intestinal microbiota and potential remediation by a novel modified HSCAS adsorbent
Authors: Yang Jia-Cheng , Lei Zhang , Liu Shuai , Chu Xiao-Han , Zhang Ni-Ya , Sun Lv-hui
Published: November 2020

The objective of the current study was to evaluate the effects of deoxynivalenol (DON) on growth performance and intestinal microbiota in weaning piglets, and potential efficacy of a modified hydrated sodium calcium aluminosilicate (HSCAS) binder to detoxify DON toxicity. Four groups of 21-day-old male piglets (n = 7/group) were fed either a control diet, or diet adding 1.0 or 3.0 mg/kg DON, or 3.0 mg/kg DON plus 0.05% modified HSCAS for 28 d. Compared to the control, dietary DON at 1.0 and/or 3.0 mg/kg decreased (P < 0.05) the feed intake (18.1–38.7%) and body weight gain (16.0–60.8%) during the whole experiment, and increased (P < 0.05) the feed/gain ratio (12.8–33.8%) during d 1–14. Worse results were obtained at 3.0 mg/kg DON in comparison to 1.0 mg/kg for most of these variables. DON exposure reshaped gut microbial structure by drastically dysregulating the abundance of several bacterial phyla, families, and genera, such as dysbiosis of Actinobacteria, Cyanobacteria, Firmicutes, and Proteobacteria in small intestine. Notably, dietary modified HSCAS binder supplementation mitigated the negative effects of DON on growth performance of piglets and improved the intestinal flora disorder. Therefore, this study has revealed that the modified HSCAS binder can mitigate DON-induced adverse effects and could be used as a promising countermeasure for detoxifying DON toxicity.
148 Effects of managing mature beef bulls on divergent planes of nutrition on body composition and concentrations of hormones and metabolites
Authors: Dahlen Carl R , Underdahl Sarah R , Crouse Matthew S , McCarthy Kacie L , Kassetas Cierrah J , Baumgaertner Friederike , Kirsch James D , Dorsam Sheri T , Sedivec Kevin K , Ward Alison K , Caton Joel
Published: November 2020

Fifteen mature beef bulls (BW = 800.4 ± 17.4 kg) were used in a 112-d experiment to evaluate effects of divergent planes of nutrition on body composition and concentrations of hormones and metabolites. Bulls were ranked by BW and randomly assigned to one of two treatments; 1) managed on a positive plane of nutrition (POS, n = 8), or 2) managed on a negative plane of nutrition (NEG, n = 7). Bulls were fed a common diet with deliveries into Insentec feeders adjusted biweekly to achieve targeted weight loss or gain (~12.5% of original BW). Blood samples were collected on d 0, 56, and 112. By design, bull BW was influenced by a treatment × day interaction (P < 0.0001), and POS bulls gained 1.27 ± 0.08 kg/d while NEG lost 0.93 ± 0.08 kg/d. Body condition score and scrotal circumference were similarly impacted by treatment × day interactions (P < 0.0001), both starting similar among treatments, then greater for POS than NEG thereafter. To achieve targeted weight divergence POS bulls (13.8 ± 0.45 kg/d) ate more (P < 0.0001) than NEG (5.1 ± 0.45 kg/d). Body composition was impacted (P ≤ 0.02), with rump fat, rib fat, loin muscle area, and intramuscular fat each increasing in POS and decreasing in NEG. Concentrations of NEFA in serum were greater in NEG bulls at d 56 and 112 compared with POS (treatment × day; P < 0.0001), concentrations of glucose (treatment × day; P = 0.02) were greater for POS bulls, and concentrations of urea were not impacted (P = 0.60). Additionally, concentrations of T3 and T4 on d 112 were greater (P ≤ 0.004) for POS. Our model resulted in altered body composition and profiles of hormones and metabolites which could influence semen on functional, morphological, and molecular levels.
153 Do pre and postnatal nutrition interact to program reproductive phenotype in sexually mature heifers?
Authors: Maia Tatiane S , Guimarães Higor R , Franco Gessica A , Pohler Ky G , Cardoso Rodolfo C , Williams Gary L
Published: November 2020

Objectives were to test the hypothesis that nutritional extremes during pregnancy in the bovine female negatively affect phenotypic characteristics of the estrous cycle of sexually mature offspring, and to determine whether these effects interact with postnatal diets during the juvenile period. Beginning at 90 d of pregnancy, Brangus and Braford (n = 108) beef cows with a female fetus were fed to achieve body condition scores of 7.5–8 (H, obese), 5–5.5 (M, moderate) or 3–3.5 (L, very thin) by the start of the third trimester. Heifer offspring were weaned and fed to gain at either a high (H; 1 kg/d) or low (L; 0.5 kg/d) rate between 4 and 8 months of age, then fed a common growth diet until puberty. Estrous cycles of a subgroup (n = 53) were synchronized using 2 injections of prostaglandin F2α(PGF). Transrectal ultrasonography was employed during the follicular phase of two estrous cycles to evaluate antral follicle count(AFC), rate of growth and size of the ovulatory follicle, size of corpus luteum(CL), and endometrial thickness. Blood samples were collected daily for assay of plasma concentrations of progesterone(P4) and estradiol-17β(E2) by RIA. Preliminary data (n = 35) were analyzed using ANOVA and mixed model procedures. Least Squares Means estrous cycle length (20.9 ± 0.2 days), AFC (19.7 ± 1.0), follicular growth rate (0.81 ± 0.11), ovulatory follicle size (12.8 ± 0.2 mm), CL size (16.8 ± 0.6 mm), and endometrial thickness (14.6 ± 0.3 mm) did not differ among treatments. Heifers in postnatal H group tended (P < 0.1) to have a greater area (arbitrary units) under the E2 curve (12.6 ± 0.6) than postnatal L (11.3 ± 0.5) during the follicular phase. Normalized AUC for P4 was greater (P < 0.03) in L/H (56.94 ± 3.22) than both M/H (42.98 ± 3.61) and L/L (43.66 ± 3.22) groups. Despite marked pre- and postnatal nutritional contrasts, independent and interactive effects of nutritional treatments have been of minor significance or undetectable to date.
152 Impact of chorionic somatomammotropin RNA interference on uterine blood flow and placental glucose uptake in the absence of intrauterine growth restriction
Authors: Tanner Amelia R , Ali Asghar , Winger Quinton A , Rozance Paul J , Anthony Russell V
Published: November 2020

Chorionic somatomammotropin (CSH) is one of the most abundant hormones produced by the sheep placenta, yet the exact function of CSH has been elusive. Previously we reported the use of in vivo RNA interference (RNAi) to assess the impact of CSH deficiency on placental and fetal growth in sheep. Near-term (135 dGA), there are two distinct CSH RNAi phenotypes: 1) pregnancies with intrauterine growth restriction (IUGR), and 2) pregnancies with normal fetal and placental weights. This study describes physiological changes in the latter phenotype. To generate the CSH RNAi pregnancies, the trophectoderm of hatched blastocysts (9 dGA) were infected with lentiviral-constructs expressing either a scrambled control (NTS) or CSH-specific shRNA (CSH RNAi), prior to transfer into synchronized recipient ewes. At 120 dGA, 6 NTS and 6 CSH RNAi pregnancies were fitted with maternal and fetal catheters. Uterine and umbilical blood flows were measured utilizing the 3H2O transplacental diffusion technique at 132 dGA, and nutrient uptakes were calculated by the Fick principle. Resulting data were analyzed by Student’s t-test and significance was set at P ≤ 0.05. CSH RNAi tended (P ≤ 0.10) to reduce placentome weight with no effect on fetal weight. Absolute (ml/min) and relative (ml/min/kg fetus) uterine blood flows were reduced (P ≤ 0.05) in CSH RNAi pregnancies, but umbilical flows were not impacted. The uterine artery-to-vein glucose gradient (mmol/l) was significantly (P ≤ 0.05) increased, whereas the gradients for taurine and glycine were reduced (P ≤ 0.05). Uteroplacental glucose uptake (mmol/min/kg placenta) was increased 27% (P ≤ 0.05), whereas umbilical glucose uptake (mmol/min/kg fetus) was reduced 13%. This cohort demonstrates that even in the absence of IUGR, CSH deficiency has significant physiological ramifications, and the investigation of CSH RNAi pregnancies exhibiting both IUGR and non-IUGR phenotypes may help determine the direct effects of CSH and its potential impact on fetal programming. Supported by NIH R01 HD093701.
158 Provision of artificial shade before weaning influences performance of Angus and Brangus cow-calf pairs
Authors: da Silva Gleise Medeiros , Schulmeister Tessa M , Podversich Federico , Tarnonsky Federico , Zamora Maria E , Urrutia Juan I , Laporta Jimena , Batista Dubeux Jose Carlos , Gonella-Diaza Angela M , DiLorenzo Nicolas
Published: November 2020

A completely randomized design study with a 2 × 2 factorial arrangement of treatments evaluated the impact of artificial shade (SHADE or NO SHADE) and breed (ANGUS vs. BRANGUS) on performance of cows, nursing calves, and subsequent offspring. Twenty-four Angus and 24 Brangus black-hided pregnant cows (579 ± 8 kg BW; 6.5 yr; approximately 85 d of gestation) and their nursing calves were randomly allocated to 12 ‘Pensacola’ bahiagrass pastures (Paspalum notatum Flüggé; 1.3 ha; n = 4 pairs/pasture), with or without access to artificial shade (NO SHADE BRANGUS [NSB], NO SHADE ANGUS [NSA], SHADE BRANGUS [SB], and SHADE ANGUS [SA]) for 56 d during summer. Body condition score (BCS) of cows and BW of pairs were obtained on d -1, 0, 55, and 56 (weaning weight). Following weaning, calves were randomly allocated to 4 pens (n = 12/pen) equipped with GrowSafe feed bunks for 14 d to measure feed intake (DMI) and efficiency (G:F). A shade × breed interaction (P < 0.05) was observed for average daily gain (ADG) and BCS of cows, with SB being greatest (P ≤ 0.05). Pre-weaning calf ADG tended to be greater (P = 0.10) for SHADE vs. NO SHADE. Weaning weight and BW 14-d post-weaning were lesser for NSB vs. NSA, SA, and SB, whereas no differences in DMI, ADG, or G:F were observed (P > 0.11). Gestation length was greater for SHADE vs. NO SHADE cows (292 vs. 274; P = 0.02), but calf birth weight was not different. Providing artificial shade to pregnant-lactating beef cows positively impacted the growth of Brangus but not Angus cows. However, weaning BW of calves from Angus cows regardless of shade access did not differ from that of Brangus calves with shade. Further research should investigate the potential long-term effects of shade on the subsequent offspring.
377 Validation and improvement of a monitoring system overtime and its overall capabilities
Authors: Jennings Tracy D , Nickell Jason
Published: November 2020

Automated animal monitoring is a growing segment in animal agriculture. These technologies capture biometric data from numerous parameters such as facial recognition, rumination, body temperature, and animal movement. The goal of automated animal monitoring technologies is to provide a 24 h perspective on an individual animal. These devices can offer valuable information pertaining to animal health, location, locomotion, animal behavior, phenotypic traits, and reproductive cycles. Devices collect data in various forms, such as counting steps, G.P.S. coordinates, triangulation, or XYZ coordinates. Raw data (e.g. step counts, XYZ coordinates) are then analyzed with proprietary algorithms to provide information in a usable format to the consumer. However, the development of these algorithms require thousands of hours of observation. For example, thorough visual observation of individual animal behaviors (e.g. rumination and eating time) can be identified based upon accelerometer movement. However, these calculated behaviors must then be validated through observation to optimize accuracy. Accelerometer-based devices are common in the dairy industry and growing in popularity in the beef industry for monitoring health and detecting estrus. Twenty-four-hour observation provides livestock producers greater insight than does once or twice daily observations. However, the usefulness or reliability of a technology requires evaluation of outcomes based on sensitivity and specificity of the test. The choice to optimize sensitivity or specificity is dependent on the desired outcome of the end user. For example, by increasing sensitivity in an animal health monitoring system, more truly ill animals will be identified, but more false positives will be generated, resulting in more animal treatments and elevated treatments costs. Conversely, increasing specificity, sick animals will be missed, but treatment costs will be reduced. Ultimately, developing an automated animal monitoring system that meets consumer expectation is the goal; however, information interpretation and evaluation will still be necessary by the end user.
202 Dietary supplementation with a blend of essential oils and a proprietary cobalt source along monensin may be antagonistic to growth performance of Holstein yearling bulls
Authors: Wu Jianping , Bai Yan , Lang Xia , Wang Cailian , Shi Xiaolei , Zhang Liping , Liu Haibo , Liu Ting , Gong Xuyin , Liang Tingyu , Zhang Rui , Casper David P
Published: November 2020

Our previous work has indicated that feeding a blend of essential oils and a proprietary cobalt source (EOC) in combination with monensin (MON) may not be mutually beneficial to dairy calf growth performance. To evaluate this observation further, a 240 d long-term growth experiment was conducted using 12 young growing Holstein bulls using a 2 × 2 factorial treatment arrangement. Main factors were EOC (Rum-A-Fresh, Ralco, Inc., Marshall, MN) and monensin (MON; Norvikon Biotechnology Co., Hunan, China) arranged in 4 treatments: 1) Control: ration fed without EOC or MON; 2) EOC: EOC fed at 26 mg/kg DM; 3) MON: MON fed at 25 mg/kg DM; and 4) EOC+MON: EOC and MON fed in combination. Holstein bulls were 70 d of age and similar in BW (93.3 ± 4.54 kg) and individually fed for 280 d. Body weights, frame measurements, and blood samples were collected monthly. Interactions of EOC by MON were detected for BW, BW gain, ADG, and a trend for feed conversion. Bulls fed EOC or MON demonstrated greater final BW (367.7, 385.2, 381.2, and 357.5 kg for Control, EOC, MON, and EOC+MON, respectively), while BW gains (277.2, 291.5, 284.8, and 265.2 kg) and ADG (1.16, 1.22, 1.19, 1.11 kg/d) were greatest for bulls fed EOC or MON compared with bulls fed EOC+MON, with bulls fed Control being intermediate and similar to bulls fed MON. Intake of DM was greater for bulls fed EOC (6.55, 6.99, 6.60, and 6.42 kg/d) compared with bulls fed remaining treatments. Feeding EOC or MON separately can improve growth performance of growing Holstein bulls. Why the combination of EOC and MON is antagonistic to growth performance of Holstein bulls is unknown.
192 Abundance of rumen methanogens did not differ in cattle that differed in residual feed intake
Authors: Freetly Harvey C , Lindholm-Perry Amanda K , Foote Andrew P , Bennett Sarah , Wells Jim E
Published: November 2020

Methane represents an energetic loss, and it has been proposed that its reduction may be associated with improved feed efficiency. Rumen methanogens produce most of the methane. Forty-nine crossbred heifers were individually fed a ration that consisted of 67.75% rolled corn, 20% wet distillers grain with solubles, 8% chopped alfalfa and 4.25% mineral/vitamin/rumensin mix for 84 d. Residual feed intake was the residual of the observed – predicted FI for the multivariate regression of FI on ADG and mid-metabolic BW for the population. Six heifers with the greatest RFI (1.75 ± 0.31 kg DM/d) and 6 with least RFI (-1.48 ± 0.31 kg DM/d) were selected. Rumen fluid was sampled from a tube passed through the mouth into the rumen. Rumen fluid was flash frozen in liquid N2 and stored at -80°C. After the first collection, heifers with greater RFI were pair fed to the less RFI heifers and rumen fluid was sampled 5 wk later. DNA was isolated from the rumen fluid and primer sets targeting total methanogens, Methanomicrobiales, Methanobacteriales, Methanosarcina, Methanobacterium, Methanobrevibacter ruminantium + Mbb. Cuticularis, and Methanobrevibacter smithii + Mbb. wolinii + Mbb. thaueri + Mbb. gottschalkii + Mbb. Woesei were amplified with real-time quantitative PCR (qPCR). Standard curves were generated to quantify copy number. There were no differences in copy number for the interaction of RFI classifications and sample period for all primer sets (P > 0.18). There were no differences in (P > 0.15) in RFI classification for all qPCR primer sets. There were fewer copies for the Methanobrevibacter ruminantium + Mbb. Cuticularis qPCR for samples collected in the first week compared to the 5 wk sample (P = 0.03); however, there were no difference for collection week for the other qPCR assays (P > 0.16). USDA is an equal opportunity provider and employer.
211 Evaluation of CLOSTAT-500® on growth performance and fecal Salmonella prevalence in yearling beef steers during the feedlot receiving phase
Authors: Smith Zachary K , Broadway Paul Rand , Sanchez Nicole C Burdick , Carroll Jeff A , Lafleur Doug , Hergenreder Jerilyn
Published: November 2020

Two-hundred and thirty eight crossbred beef steers (initial BW = 419 ± 32.4 kg) were used to evaluate the influence of a patented probiotic on growth performance responses and fecal Salmonella prevalence during a 28-d feedlot receiving period at the South East Research Farm (SERF) feedlot located near Beresford, SD. Steers were allotted to 24 pens (9 to 10 steers/pen) and assigned to one of two treatments (12 pens/treatment): no probiotic (CON) or 0.5 g·steer-1·d-1 of a Bacillus subtilis PB6 probiotic (CLOSTAT-500®, Kemin Industries, Des Moines, IA; CLO). Steers were transitioned from a 70% concentrate diet (DM basis) to a 92% concentrate diet (DM basis) over a 14-d period. The final diet contained (DM basis): 12.5% CP, 2.08 Mcal/kg of NEm, 1.40 Mcal/kg of NEg, 30 g/907-kg of monensin sodium, and 0.45 mg/kg of chromium propionate (KemTRACE® Chromium, Kemin Industries, Des Moines, IA). Fecal samples were collected on study d 1 and 28 (6 and 34 d following arrival to the SERF, respectively) from sentinel steers (n = 5/pen) via rectal palpation and composited by pen for determination of Salmonella prevalence using selective enrichment and culture medias. Steers were fed once daily and bunks were managed according to a slick bunk management approach. Data were analyzed as a randomized complete block design; pen served as the experimental unit and an α of 0.05 determined significance. No differences were detected (P ≥ 0.25) between treatments for ADG, DMI, or gain efficiency during the initial 28-d feedlot receiving phase. Additionally, no Salmonella was recovered in any fecal samples collected. These data indicate that Bacillus subtilis PB6 had no influence on receiving phase growth performance, and fecal Salmonella prevalence was not observed in yearling steers placed on feed in March in southeastern South Dakota.
196 Screening several phytogenic compounds for their effect on ruminal fermentation parameters in cattle fed high grain diet
Authors: Ricci Sara , Petri Renee M , Castillo-Lopez Ezequias , Rivera-Chacon Raul , Reisinger Nicole , Zebeli Qendrim
Published: November 2020

Phytogenic compounds, e.g. secondary plant compounds and essential oils, are often used as supplements in ruminant nutrition to modulate microbial fermentation under ruminal dysbiosis associated with high grain feeding. The aim of this study was to evaluate the short-term effects of phytogenic compounds on volatile fatty acids (VFA) production and composition in cows affected by subacute ruminal acidosis (SARA). Nine substances were tested at low and high dosage (1x and 10x respectively) using nine non-lactating ruminally cannulated Holstein cows, adapted to a 65% concentrate and 35% silage (grass and corn) ration (DM basis). Feeding was restricted for nine hours before providing the substance mixed with five kg of TMR. Each substance and dosage was tested individually on four cows (n = 4/compound/dose) over a period of four hours. Rumen and reticulum fluid were collected before the treatment, and four hours after feeding. VFA were measured with gas chromatography. Ruminal pH was continuously recorded (eCow indwelling pH systems). Data were analyzed with the Proc Mixed procedure of SAS. Seven out of nine compounds showed an effect or a trend on VFA measured, both in rumen and reticulum. Acetic acid in rumen fluid decreased with garlic oil, while low dose mint oil increased its concentration (P = 0.06 and P = 0.01, respectively). Low dose mint oil (P < 0.01) and L-menthol (P = 0.03) both increased the A:P ratio, whereas garlic oil tended towards a decrease in the A:P ratio. Garlic oil and ginger showed negative correlations between total VFA and mean ruminal pH (P = 0.03 and P < 0.01, respectively). Our results suggest that phytogenic compounds can modulate microbial activity within four hours of feeding. The use of phytogenic compounds may have beneficial effects on microbial fermentation under low pH conditions associated with high grain feeding.
217 Assessment of the dissolution characteristics of Rumensin® 90 and Monovet® 90
Authors: Fuller Gary B , Foust Laura , Krabel Beverly , Brown Mike S
Published: November 2020

Continued evaluation of physical attributes of monensin Type A medicated articles is needed to keep customers apprised of product characteristics. Two randomly chosen lots of Rumensin® 90 (P1 and P2) and Monovet® 90 (A1 and A2) with dates of manufacture between May and September 2019 were used to evaluate dissolution properties according to US Pharmacopeia in vitro procedures and FDA guidance for interpretation of dissolution data. A synthetic rumen fluid (lacking microorganisms) that simulated an animal fed a high-forage diet (pH = 6.8) was used in Exp. 1, whereas a synthetic rumen fluid simulating an animal fed a high-concentrate diet (pH = 5.5) was used in Exp. 2. Samples were added to dissolution vessels containing 1 L of the appropriate media and maintained at 39°C with constant stirring (250 rpm). Aliquots (10 mL) were collected after 15, 30, 45, 60, 90, 120, 240, 360, and 480 min, filtered, and subsequently analyzed for monensin by HPLC procedures. The Rumensin lot with the lowest dissolution was designated as the reference (P2); A1 and A2 were compared to this reference using f1 and f2 test statistics computed following FDA guidance. Dissolution profile comparisons were considered statistically different if the f1 statistic was > 15 and the f2 statistic was < 50. In both experiments, Monovet dissolution profiles were lower than Rumensin profiles. In Exp. 1, f1 = 45 and 50 and f2 = 23 and 20 for A1 and A2, respectively. Likewise in Exp. 2, f1 = 41 and 27 and f2 = 31 and 41 for A1 and A2, respectively. Although the Monovet and Rumensin dissolution profiles were deemed similar in the studies used for FDA approval of Monovet, these data indicate that they are not similar using the most recent industry-standard procedures.
230 Effects of encapsulated calcium-ammonium nitrate on performance and methane production of beef steers grazing mixed winter forage
Authors: Doblado Andrea M Osorio , Mejia-Turcios Sebastian E , Stotz Miranda K , Vargas David , de Araujo Rafael Canonenco , DiLorenzo Nicolas , Henry Francine , Sarturi Jhones Onorino , Henry Darren D
Published: November 2020

The effects of encapsulated calcium-ammonium nitrate (eCAN) on in vivo methane production and performance were evaluated. A generalized randomized block design was used with beef steers grazing a mixed winter forage (Triticum aestivum, Triticosecale rimpaui, and Secale cereale) for 49 d. Thirty-six Angus-crossbred steers (332 ± 53 kg) were blocked by BW and randomly assigned to 1 of 3 treatments: 1) winter pasture + corn (WC), 2) WC + 328 mg/kg of BW eCAN (WCN) and 3) WC + 124 mg/kg of BW UREA (WCU), all supplemented with corn (0.3% BW treatments). Treatments WCN and WCU were isonitrogenous. A 14d adaptation period was used to adapt cattle to treatments. Methane emissions were measured using the sulfur hexafluoride tracer technique. Blood samples and BW were taken at d 0, 24, 35, and 49. Supplemental corn and NPN orts were collected daily. Data were analyzed using the MIXED procedure of SAS with the fixed effect of treatment and random effect of pasture (block); BUN, supplement intake and CP intake were analyzed with repeated measures. Steer was considered the experimental unit. Methane production was not different (P > 0.05) considering g/d, g/kg of BW, g/kg of MBW, or g/kg of ADG. Treatments did not affect ADG (P = 0.941). Supplement intake was affected by treatment (P < 0.001), with WC (0.979 kg) being greater compared to WCU (0.887 kg) and WCN (0.706 kg). Total CP intake increased (P < 0.001) with WCU (0.155 kg) and WCN (0.116 kg), compared to WC (0.074 kg), which did not have a non-protein source. Blood urea nitrogen was affected by day, with d 24 (18.598 mg/dL) being greater compared to d 0 (8.215 mg/dL), 35 (10.549 mg/dL) and 49 (14.5744 mg/dL). The eCAN did not effectively replace urea as a NPN source to mitigate enteric methane.
392 Vitamin C and transit stress in beef cattle
Authors: Deters Erin L , Beenken Aubree M , Hansen Stephanie L
Published: November 2020

Transportation of cattle by road is unavoidable in beef production due to segmentation of the industry. Psychological and physical stress associated with transit can negatively impact cattle health and performance upon arrival at their destination. Thus, investigation of resiliency or recovery-based nutritional strategies to mitigate transit stress are warranted. Because oxidative stress is likely linked to transportation stress, one such strategy is antioxidants such as vitamin C (VC). Seventy-two Angus-cross steers (356 ± 18 kg) were used to determine the effects of a pre- versus post-transit VC injection on 56 d feedlot performance. Steers were randomly assigned to intramuscular injection treatments (24 steers/treatment): saline pre- and post-transit (CON), VC (Vet One; 250 mg sodium ascorbate/mL; 5 g sodium ascorbate/steer) pre-transit and saline post-transit (PRE), or saline pre-transit and VC post-transit (POST). Steers were transported for ~18 h (1,675 km) prior to sorting into pens equipped with GrowSafe bunks (6 steers/pen). Blood was collected from 12 steers/treatment on d 0, 1, 2, and 7 for analysis of plasma ascorbate concentrations. Plasma ascorbate concentrations were decreased by ~10% immediately post-transit for CON and POST-steers but increased for PRE-steers; regardless of treatment, concentrations were similar to pre-transit values by d 7 (treatment × day; P < 0.01). Steers that received VC at either timepoint (pre- or post-transit) exhibited greater dry matter intake from d 31–57 and d 1–57 compared to CON-steers (P ≤ 0.02). However, PRE-steers exhibited the greatest average daily gain from d 7–31 and d 1–57 (P ≤ 0.05), resulting in PRE-steers being heaviest on d 30/31 (P = 0.03) and tending to be heaviest on d 56/57 (P = 0.07). Based on these data, timing of nutritional intervention is vital for optimal effectiveness. Furthermore, nutritional strategies that improve antioxidant status prior to long-distance transit events may positively influence post-transit performance.
401 Gut microbiome, health, and growth performance in pigs
Authors: Farzan Vahab
Published: November 2020

As pigs grow, their intestinal microbiome evolves until establishing a stable rich and diverse microbial population (climax community) to maintain the health and growth performance. This evolution (microbial succession) is in fact a multifactorial phenomenon involving environmental parameters (farm practice measures, heard health status, drug use), host (genetics, immune system, biomarkers), sow (milk, colostrum, vaginal, and rectal microbiome) and nutrition. The multifactorial nature of microbial succession and the consequence disruption results in a considerable variation in the pig gut mirobiome at the individual and herd level. This needs to be taken into account when exploring the core bacterial population associated with health and growth performance in pigs under commercial farming conditions.
251 Impacts of different levels of dietary zinc oxide on mitochondrial energy metabolism and oxidative stress conditions in post-weaned piglets
Authors: Lapointe Jerome , Roy Caroline , Deschene Karine , Novais Aliny , Audet Isabelle , Martel-Kennes Yan , Guay Frédéric , Talbot Guylaine , Lessard Martin , Dalto Danyel B , Matte J Jacques
Published: November 2020

Zinc oxide (ZnO) is commonly added to weanling piglet diets for its documented benefits as growth promoting agent and usefulness for preventing diarrhea. We have recently reported the occurrence of mitochondrial energetic deficiencies and oxidative stress conditions in post-weaned piglets. In cells, Zn is known to be primarily located in mitochondria, where it is involved in mitochondrial function. The objective of this study was to determine the impacts of different levels of dietary ZnO on mitochondrial function and oxidative stress. One hundred twenty suckling piglets (5.65 ± 0.68 kg) were selected at d14. After weaning (d21), they were fed a basal diet supplemented with 100 mg/kg (low; L), 1000 mg/kg (medium; M) or 3000 mg/kg (high; H) of ZnO until d42 (n = 40/treatment). Piglets were sacrificed at d21, d23, d35, and d42 of age (n = 10/treatment/d) for sample collection. Mitochondrial respiration was evaluated using an extracellular flux analyzer and our results showed higher oxygen consumption rates in liver mitochondria from H group (P < 0.05) at d42. The cellular energy status was characterized by measuring liver adenosine 5’-triphosphate (ATP) content, and lower levels were reported in H group (P < 0.01) at d42. These observations were concomitant with a decrease in activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) in cytosol and mitochondria. Quantitative RT-PCR analyses revealed that expression of genes implicated in oxidative stress response such as glutathione synthase (GSS), heme oxygenase 1 (HO1) and metallothionein 3 (MT3) is increased in H group at d42 (P < 0.05). In plasma, GPx activity and oxidative damage to proteins (carbonyls) was higher in H group (P < 0.05). These findings indicate that ZnO affects mitochondrial function and oxidative stress in post-weaned piglets and provide insights to guide the prophylactic practices using high levels of dietary ZnO.
267 Supplementing Ca salts of soybean oil to late-gestating beef cows: Impacts on performance and physiological responses of the offspring
Authors: Brandão Alice , Cooke Reinaldo F , Schubach Kelsey , Rett Bruna , Souza Osvaldo , Pohler Ky G , Bohnert David , Marques Rodrigo
Published: November 2020

This experiment compared performance and physiological responses of the offspring from cows supplemented with Ca salts of soybean oil (CSSO) or prilled saturated fat (CON) during late gestation. Non-lactating, pregnant Angus × Hereford cows (n = 104) that conceived during the same fixed-time artificial insemination protocol, using semen from 2 sires, were used in this experiment. Cows were ranked by pregnancy sire, body weight (BW), and body condition score (BCS). On d 180 of gestation (d -15), cows were randomly assigned to receive (dry matter basis) 415 g of soybean meal per cow daily in addition to 1) 195 g/cow daily of CSSO (n = 52) or 2) 170 g/cow daily of CON (n = 52). Cows were maintained in 2 pastures (26 cows/treatment per pasture), and received daily 12.7 kg/cow (dry matter basis) of grass-alfalfa hay. From d 0 until calving, cows were segregated into 1 of 24 feeding pens thrice weekly and received treatments individually. Cow BW and BCS were recorded, and blood samples were collected on d -15 of the experiment and within 12 h after calving. Calf BW was also recorded and blood sample collected within 12 h of calving. Calves were weaned on d 290 of the experiment, preconditioned for 35 d (d 291 to 325), and transferred to a feedyard where they remained until slaughter. Upon calving, CSSO cows and calves had greater (P < 0.01) plasma concentrations of linoleic acid and total ω - 6 FA compared with CON cohorts. No differences in calf birth BW, weaning BW, and final preconditioning BW were noted (P ≥ 0.36) between treatments. Average daily gain and final BW in the feedlot were greater (P ≤ 0.05) in steers from CSSO cows compared with CON. The incidence of calves diagnosed with BRD that required a second antimicrobial treatment was less (P = 0.03) in calves from CSSO cows, resulting in reduced (P = 0.05) need of treatments to regain health compared with CON (Table 5). Upon slaughter, longissimus muscle area was greater (P = 0.03) in calves from CSSO cows compared with CON. Collectively, these results suggest that supplementing CSSO to late-gestating beef cows stimulated programming effects on postnatal offspring growth and Page 2 of 15 For Peer Review health. Therefore, supplementing late-gestating beef cows with CSSO appears to optimize offspring welfare and productivity in beef production systems.
266 Administering an appeasing substance to beef calves at weaning to optimize welfare and productivity
Authors: Schubach Kelsey , Cooke Reinaldo F , Brandão Alice , Rett Bruna , Ferreira Vitor , Scatolin Giovanna , Colombo Eduardo , Daigle Courtney L , Pohler Ky G , Cappellozza Bruno I
Published: November 2020

This experiment evaluated the impacts of bovine appeasing substance (BAS) administration on performance, behavioral, and physiological responses of beef calves upon weaning. Eighty Angus-influenced calves (40 heifers, 40 steers) were weaned at 233 ± 2 d of age (d 0), ranked by age and body weight (BW), and assigned to receive BAS (Nutricorp, Araras, SP, Brazil; n = 40) or placebo (diethylene glycol monoethyl ether; CON; n = 40). Treatments (5 mL) were topically applied to the nuchal skin area of each animal. Within treatment, calves were allocated to 1 of 8 drylot pens (4 pens/treatment) and received a free-choice total mixed ration (TMR) from d 0 to 42, intake of which was assessed daily. On d 0, calves were fitted with a pedometer behind their right shoulder, and pedometer results were recorded weekly during the experiment (d 0 to 42) concurrently with full BW collection and temperament evaluation. Blood samples were collected on d 0, 3, 7, 14, 28, and 42, and hair samples were collected from the tail switch on d 0, 14, 28, and 42. Average daily gain from d 0 to 42 did not differ between treatments (P = 0.52), but was greater (P = 0.05) in BAS vs. CON calves from d 0 to 28. Intake of TMR was greater during the first week for BAS vs. CON calves (P = 0.05), and similar (P ≥ 0.44) from wk 2 to 6 (treatment × week; P = 0.08). Exit velocity was greater (P = 0.03) for CON vs. BAS calves on d 14 and tended (P = 0.08) to be greater for CON vs. BAS calves on d 7 (treatment × day; P = 0.04). Physical activity was greater for CON vs. BAS calves on d 1 (P < 0.01), but greater (P = 0.01) in BAS vs. CON on d 2 (treatment × day; P = 0.01). Mean plasma concentrations of haptoglobin were greater (P = 0.03) in CON vs. BAS calves during the experiment. Hair cortisol concentrations were greater (P = 0.0 5) in CON vs. BAS calves on d 14 (treatment × day interaction; P = 0.03). Results from this experiment indicate BAS calves habituated to their environment more rapidly through increased physical activity, which facilitated a lessened stress response associated with weaning, and accelerated adaptation to a novel environment. This was manifested through improved TMR intake and BW gain during the initial period of the experiment. Collectively, results suggest use of BAS as a strategy to improve calf welfare and productivity upon weaning.
261 Sorting strategy and effect of variation on profitability of a pen of feedlot steers
Authors: Harrison Meredith A , Oltjen James W
Published: November 2020

Variation in feedlot cattle has a negative effect on feedlot profitability and beef products. Alternative sorting strategies may help reduce variation within a pen of feedlot cattle and improve beef product uniformity. A dynamic, deterministic model was built to predict growth, body composition, and carcass value for individual steers. Individual steer measurements for initial body weight (BW), frame size, and body fat percent were obtained from a set of Angus-based steers (n = 62; BW = 293kg) and used as initial values in the model. The model predicted individual steer BW, Yield and Quality Grades, and carcass values for each day in the feeding period. Total steer profit was calculated as carcass value minus feed costs and steer purchase price. Profitability was evaluated using four sorting strategies: 1) steers purchased, fed, and marketed as a lot without sorting; 2) steers purchased, sorted by BW at initial processing, and fed and marketed as two independent pens; 3) steers purchased and fed as a lot until midway through the feeding period, and then were sorted by BW into two pens that were marketed independently; and 4) steers purchased and fed as a lot and each individual steer was sold at the optimal time. Optimal marketing time was defined as the day profit reached a maximum value. Sensitivity analyses were conducted, changing all prices ± 10% while all other variables were held constant. Scenario 4 was most profitable, with a mean profit of $161.78 ± 144.79 per steer, representing the greatest possible profit. Scenario 1, the practice commonly used by commercial feedlots, was the least profitable ($122.45 ± 139.65). Scenario 2 ranked third ($125.10 ± 137.42), and Scenario 3 ranked second ($127.85 ± 137.73), but mean profit per head only differed by $2.75, indicating steers grew at a relatively constant rate, and there was no additional benefit in sorting mid-way through the finishing period versus at initial processing. Body weight at harvest and carcass weight varied across scenarios, but mean quality grade was similar for all four scenarios. Mean profit per was most sensitive to grid base price. In scenario 1, a ± 10% change in grid base price resulted in a 145 and –136% profit change, respectively. Sorting feedlot steers into similar feeding and marketing groups reduces pen variation, increasing carcass value, decreasing feed costs, and improving overall profitability.
PSV-4 Correlation between superficial body temperatures measured with an infrared thermometer and rectal temperature in alpacas
Authors: Vilela Jorge L , Goycochea Gianella , Serna Allison , Veramendi Frank , Ascue Jorge , Callan Milagros , Jauregui Andrea , Miranda Almendra , Montalvo Pamela , Sandoval Aranza , Solis Sebastian
Published: November 2020

Temperature measurement is essential during physical examination since it helps to detect abnormalities in animal health. Moreover, measurement should be done within the shortest possible time to avoid stress. The objective of this study was to determine the correlation between rectal temperature and the superficial body temperature using a digital infrared thermometer. Ten Huacaya alpacas from the DESCOSUR Center of Alpaca Development in Toccra, located in the Arequipa region, Perú at 4,365 m above sea level (MASL), and eight Huacaya alpacas from the Zootechnical and Technological Unit of the Universidad Cientifica del Sur, in Lima at 5 MASL, were studied. Rectal temperatures were obtained using a veterinary clinical thermometer for one minute. Superficial body temperature measurement was carried out with a CENTER 350® infrared thermometer, at a distance between 10 to 25 cm from the measured site. Six measuring points were used. Rectal temperatures were measured at the same time as the superficial body temperatures. The Statistical Analysis System (SAS®) University Edition, 2018 was used to perform Pearson’s correlation coefficient and graphs were made using the CORRGRAM R software package. Correlations with rectal temperature were significantly (P < 0.01) higher with the middle zone of the ear and the outer zone of the nose (0.75 and 0.69, respectively). In conclusion, the results in the present study indicated a significant positive correlation between rectal and infrared temperatures at different anatomical points in alpacas. Highly significant correlations were observed between the rectal temperature and the middle zone of the ear and the outer zone of the nose. These results could be used to perform an alternative method to predict body temperature by infrared temperature in alpacas; however, further testing is required in the field to validate these estimates.
PSV-15 Influence of the temperament of water buffaloes on the serum lipid levels
Authors: Francisco Caroline L , Castilhos André M , da Silva Daiane C Marques , da Silva Fabiola Martinez , Aranha Aline S , Jacaúna Amanna G , Luz Patricia A C , Paula Tania V , Oliveira Camila S , Jorge Andre M
Published: November 2020

This study evaluated the effect of the temperament of 75 water buffaloes of three genetic groups (GG: Jafarabadi, Mediterranean, and Murrah; n = 25 for each GG) on serum lipid levels. The animals were classified for temperament through the temperament score (TSc) obtained by the mean of the sum of the scores of the entry time into the squeeze chute (1 to 5: 1=greater time spent for entry, and 5=less time spent for entry) and the exit velocity score (1 to 5: 1=lower speed, and 5=higher speed) calculated after the period of adaptation to the feedlot (d0). The animals were categorized into adequate (ADQ; TSc≤3) or excitable (EXC; TSc >3) temperaments. Blood samples were collected from the jugular vein on days -28, 0, and 84, and evaluated for serum levels of total cholesterol, triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) using colorimetric enzymatic analyses performed by commercial kits. Data were analyzed using MIXED procedure in SAS, considering the GG, temperament, day, and the resulting interactions as fixed effects. The term day was used for repeated measures in time. Correlations were estimated using the CORR procedure in SAS. There were no interactions for the variables evaluated (P ≥ 0.11). ADQ animals showed lower levels of total cholesterol (65.95 vs 70.96mg/dL for ADQ and EXC, respectively; SEM=2.33; P = 0.04) and LDL (26.74 vs 30.40mg/dL for ADQ and EXC, respectively; SEM=1.57; P = 0.02) than EXC animals. There was no effect of temperament for the triglycerides (P = 0.41), HDL (P = 0.47), and VLDL (P = 0.46). Positive correlations were verified between both the TSc and triglycerides (r=0.32; P < 0.01), and TSc and VLDL (r=0.33; P < 0.01). Tendency for a positive correlation was detected between TSc and LDL (r=0.20; P = 0.09). In conclusion, there are differences in serum lipid levels between water buffalo temperaments which deserves further investigation. Supported by FAPESP (#2018/25939-1; #2014/05473-7).