bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Hydroxyselenomethionine with Symmetrical and Chelated Chemical Structure on Lactation Performances, Anti-Oxidative Status and Immunities, Selenium Transfer Efficiencies for Early-Lactating Dairy Cows
Authors: Li Yong , Zhang Weixian , Zhou Hualin , Zhu Jinfeng , Pan Chunmei
Published: May 2022

The current study was conducted to investigate effects of hydroxyselenomethionine (HMBSe) with symmetrical and chelated chemical structure, a novel organic selenium (Se) source, on lactation performance, anti-oxidative status and immunities, and transfer efficiencies for early lactation dairy cows compared with that of sodium selenite (SS). Forty-five multiparous early-lactating dairy cows with similar days in milk, 56.0 d and milk yield 36.1 kg/d, were fed with same basal diet containing 0.04 mg of Se/kg of dry matter (DM) basis. They were assigned to 1 of 3 treatments according to one-way ANOVA design: control (basal diet, without Se supplementation), SS (0.30 mg of Se/kg of DM), or HMBSe (0.30 mg of Se/kg of DM). The experiment lasted for 9 weeks, with the first week as adaptation. Results showed that the organic HMBSe cows increased the milk yield, 4% fat-corrected milk yield, the numbers of red blood cells in whole blood, Se concentrations in milk and serum, ratio of milk to serum significantly (P < 0.01); feed efficiency, energy-corrected milk yield, contents of superoxide dismutase in serum, hemoglobin, and the numbers of white blood cells in whole blood significantly (P < 0.05) compared to control and SS. Moreover, HMBSe cows had trends to increase glutathione peroxidase activities (P = 0.09), total antioxidant capacity (P = 0.06), and had trends to decrease the contents of malonaldehyde (P = 0.07) in serum compared to control and SS. In conclusion, HMBSe was more effective on the lactation performances, anti-oxidative status, and immunities and Se transfer efficiencies for early-lactating dairy cows compared to control and SS, which was very meaningful to develop the enriched Se milk products.
A Boundedly Rational Decision-Making Model Based on Weakly Consistent Preference Relations
Authors: Wu Xinlin , Xiao Haiyan
Published: May 2022

Completeness is one of the basic assumptions about the rational preference relation in classical decision theory. Strongly and weakly consistent preferences are presented by abandoning the completeness of the rational preference relation. Some expansion and contraction conditions are proposed and the relationships between these conditions of rationality are discussed. The relationships between the conditions of rationality and boundedly rational choice behavior based on strongly and weakly consistent preferences are analyzed and discussed. Furthermore, an example about the choices of chocolates with interval ordinal numbers is given to explain some of the main conclusions in this paper. The results can be used as references for the study of boundedly rational decisions.
Predictive Modelling of Statistical Downscaling Based on Hybrid Machine Learning Model for Daily Rainfall in East-Coast Peninsular Malaysia
Authors: Sulaiman Nurul Ainina Filza , Shaharudin Shazlyn Milleana , Ismail Shuhaida , Zainuddin Nurul Hila , Tan Mou Leong , Abd Jalil Yusri
Published: May 2022

In recent years, climate change has demonstrated the volatility of unexpected events such as typhoons, flooding, and tsunamis that affect people, ecosystems and economies. As a result, the importance of predicting future climate has become even direr. The statistical downscaling approach was introduced as a solution to provide high-resolution climate projections. An effective statistical downscaling scheme aimed to be developed in this study is a two-phase machine learning technique for daily rainfall projection in the east coast of Peninsular Malaysia. The proposed approaches will counter the emerging issues. First, Principal Component Analysis (PCA) based on a symmetric correlation matrix is applied in order to rectify the issue of selecting predictors for a two-phase supervised model and help reduce the dimension of the supervised model. Secondly, two-phase machine learning techniques are introduced with a predictor selection mechanism. The first phase is a classification using Support Vector Classification (SVC) that determines dry and wet days. Subsequently, regression estimates the amount of rainfall based on the frequency of wet days using Support Vector Regression (SVR), Artificial Neural Networks (ANNs) and Relevant Vector Machines (RVMs). The comparison between hybridization models’ outcomes reveals that the hybrid of SVC and RVM reproduces the most reasonable daily rainfall prediction and considers high-precipitation extremes. The hybridization model indicates an improvement in predicting climate change predictions by establishing a relationship between the predictand and predictors.
Asymmetric Free-Hand Interaction on a Large Display and Inspirations for Designing Natural User Interfaces
Authors: Lou Xiaolong , Chen Ziye , Hansen Preben , Peng Ren
Published: May 2022

Hand motion sensing-based interaction, abbreviated as ‘free-hand interaction’, provides a natural and intuitive method for touch-less interaction on a large display. But due to inherent usability deficiencies of the unconventional size of the large display and the kinematic limitations of the user’s arm joint movement, a large display-based free-hand interaction is suspected to have different performance across the whole areas of the large display. To verify this, a multi-directional target pointing and selection experiment was designed and conducted based on the ISO 9241-9 evaluation criteria. Results show that (1) free-hand interaction in display areas close to the center of the body had a higher accuracy than that in peripheral-body areas; (2) free-hand interaction was asymmetric at the left side and the right side of the body. More specifically, left-hand interaction in the left-sided display area was more efficient and accurate than in the right-sided display area. For the right-hand interaction, the result was converse; moreover, (3) the dominant hand generated a higher interaction accuracy than the non-dominant hand. Lessons and strategies are discussed for designing user-friendly natural user interfaces in large displays-based interactive applications.
Analytically Pricing Formula for Contingent Claim with Polynomial Payoff under ECIR Process
Authors: Nualsri Fukiat , Mekchay Khamron
Published: May 2022

Contingent claims, such as bonds, swaps, and options, are financial derivatives whose payoffs depend on uncertain future real values of underlying assets which emphasize various real-world applications. In general, valuations for contingent claims can be derived from the conditional expectations of underlying assets. For a simple process, the moments are usually directly obtained from its transition probability density function (PDF). However, if the transition PDF is unavailable in simple form, the derivations of the moments and the contingent claim prices will not be accessible in closed forms. This paper provides a closed-form formula for pricing contingent claims with nonlinear payoff under a no-arbitrage principle when underlying assets follow the extended Cox–Ingersoll–Ross (ECIR) process with the symmetry properties of the Brownian motion. The formula proposed here is a consequence of successfully solving an explicit solution for a system of recurrence partial differential equations in which its solution subtly depends on the conditional moments. For the particular CIR process, we obtain simple closed-form formulas by solving the Riccati differential equation. Furthermore, we carry out a complete investigation of the convergent case for those formulas. In case such as that of the unsolvable Riccati differential equation, ECIR case, a numerical method for numerically evaluating the mentioned analytical formulas and numerical validations for the formulas are examined. The validity and efficiency of the formulas are numerically demonstrated by comparison with results from Monte Carlo simulations for various modeling parameters. Finally, the proposed formula is applied to the value contingent claims such as coupon bonds, interest rate swaps, and arrears swaps.
Using Machine Learning to Profile Asymmetry between Spiral Galaxies with Opposite Spin Directions
Authors: Shamir Lior
Published: May 2022

Spiral galaxies can spin clockwise or counterclockwise, and the spin direction of a spiral galaxy is a clear visual characteristic. Since in a sufficiently large universe the Universe is expected to be symmetric, the spin direction of a galaxy is merely the perception of the observer, and therefore, galaxies that spin clockwise are expected to have the same characteristics of galaxies spinning counterclockwise. Here, machine learning is applied to study the possible morphological differences between galaxies that spin in opposite directions. The dataset used in this study is a dataset of 77,840 spiral galaxies classified by their spin direction, as well as a smaller dataset of galaxies classified manually. A machine learning algorithm was applied to classify between images of clockwise galaxies and counterclockwise galaxies. The results show that the classifier was able to predict the spin direction of the galaxy by its image in accuracy higher than mere chance, even when the images in one of the classes were mirrored to create a dataset with consistent spin directions. That suggests that galaxies that seem to spin clockwise to an Earth-based observer are not necessarily fully symmetric to galaxies that spin counterclockwise; while further research is required, these results are aligned with previous observations of differences between galaxies based on their spin directions.
Spontaneous Retroperitoneal Hematoma Treated with Percutaneous Transarterial Embolization in COVID-19 Era: Diagnostic Findings and Procedural Outcome
Authors: Tiralongo Francesco , Seminatore Salvatore , Di Pietro Stefano , Distefano Giulio , Galioto Federica , Vacirca Francesco , Giurazza Francesco , Palmucci Stefano , Venturini Massimo , Scaglione Mariano , Basile Antonio
Published: May 2022

(1) Background: Spontaneous retroperitoneal hematomas are a relatively common occurrence in hospitalized patients with COVID-19 related pneumonia, and endovascular treatment of trans-arterial embolization (TAE) may be a life-saving procedure after failure of medical and supportive therapy. The aim of our study was to evaluate spontaneous retroperitoneal hematomas in the COVID-19 era, focusing on their imaging features at CTA and DSA and on the safety, as well as technical and clinical success, of TAE, comparing patients affected by COVID-19 and non-COVID-19 patients. (2) Materials and Methods: We retrospectively enrolled 24 patients with spontaneous retroperitoneal hematoma who underwent TAE; of these, 10 were hospitalized for COVID-19-related pneumonia, while the other 14 were without COVID-19 infection. We evaluated the demographic data, hemoglobin values before and after the procedure, preprocedural aPTT, preprocedural INR, diagnostic and interventional imaging findings, procedural outcome (technical success) and survival periprocedural (clinical success), and major and minor complications. (3) Results: The mean age of the study population was 72.7 ± 11.2 years. CTA revealed signs of active bleeding in 20 patients (83%). DSA showed signs of active bleeding in 20 patients (83%). In four patients (17%), blind embolization was performed. The overall technical success rate was 100%. Clinical success was achieved in 17 patients (71%), while seven patients (29%) rebled within 96 h, and all of them were retreated. No major periprocedural complication was reported. The comparison between the two groups did not show statistically significant differences for gender, mean age, mean pre- and postprocedural hemoglobin, aPTT and INR, mean hematoma volume (cm3), or mean delay between CT and DSA. Active bleeding at CTA was detected in 90% of COVID-19 patients and 79% of non-COVID-19 patients (p = 0.61). At DSA, active bleeding was assessed in eight out of 10 (80%) patients in the COVID-19 group and 12 out of 14 (86%) patients in the non-COVID-19 group (p = 1). Technical success was obtained in 100% of patients in both groups. Clinical success rates were 70% for COVID-19 group and 71% for the non-COVID-19 group. We found no statistical significance between the clinical success rates of retroperitoneal spontaneous hematoma embolization in patients with or without SARS-CoV-2 infection. (4) Conclusions: We suggest that, similar to what has been reported in other studies in non-COVID-19 patients, TAE should be considered an important safe, effective, and potentially life-saving option for the management and the treatment of patients affected by COVID-19 who present with spontaneous retroperitoneal hematoma and who could not benefit from conservative treatment.
The H Index Myth: A Form of Fanaticism or a Simple Misconception?
Authors: Quaia Emilio , Vernuccio Federica
Published: May 2022

Bibliometry represents a branch of library and information sciences that uses statistical methods to analyse publications [...]
Oxidative Stress and Histopathological Changes in Gills and Kidneys of Cyprinus carpio following Exposure to Benzethonium Chloride, a Cationic Surfactant
Authors: Gheorghe Stefania , Stan Miruna S. , Mitroi Daniel N. , Staicu Andrea C. , Cicirma Marius , Lucaciu Irina E. , Nita-Lazar Mihai , Dinischiotu Anca
Published: May 2022

One cationic surfactant with a wide spectrum of microbiocidal activity is benzethonium chloride (BEC). Despite being widely used, the toxicity data on vertebrate organisms are limited. Therefore, we aimed to evaluate within this study the acute toxicity of BEC on the gills and kidneys of Cyprinus carpio (European carp). An alteration of the antioxidant enzymes activities (glutathione reductase, glutathione peroxidase and glutathione S-transferase) was noticed after 96 h of exposure, along with an elevation of lipid peroxidation and decreased concentration of reduced glutathione, which confirmed that BEC was able to induce toxicity to these tissues. These metabolic effects were correlated with unspecific structural changes observed in gills and kidneys, having moderate degree of severity (such as an increase of melanomacrophages aggregation incidence and cytoplasm vacuolation of goblet cells in collecting tubules) and generally being compatible with life for the exposure time studied. The most severe structural effects were observed in gills after 96 h, noticing a lamellar aneurysm, hemorrhages and lamellar epithelium disruption due to the blood vessels and pillar cells damages and increased blood flow inside the lamellae. By our research we can confirm the utility of biochemical and histological analyses in the fish organs as tools for monitoring the water quality and ecotoxicological potential of chemicals.
Environmental Occurrence and Predicted Pharmacological Risk to Freshwater Fish of over 200 Neuroactive Pharmaceuticals in Widespread Use
Authors: Sumpter John P. , Margiotta-Casaluci Luigi
Published: May 2022

There is a growing concern that neuroactive chemicals released into the environment can perturb wildlife behaviour. Among these chemicals, pharmaceuticals such as antidepressants and anxiolytics have been receiving increasing attention, as they are specifically prescribed to modify behavioural responses. Many laboratory studies have demonstrated that some of these compounds can affect various aspects of the behaviour of a range of aquatic organisms; however, these investigations are focused on a very small set of neuroactive pharmaceuticals, and they often consider one compound at a time. In this study, to better understand the environmental and toxicological dimension of the problem, we considered all pharmaceuticals explicitly intended to modulate the central nervous system (CNS), and we hypothesised that these compounds have higher probability of perturbing animal behaviour. Based on this hypothesis, we used the classification of pharmaceuticals provided by the British National Formulary (based on their clinical applications) and identified 210 different CNS-acting pharmaceuticals prescribed in the UK to treat a variety of CNS-related conditions, including mental health and sleep disorders, dementia, epilepsy, nausea, and pain. The analysis of existing databases revealed that 84 of these compounds were already detected in surface waters worldwide. Using a biological read-across approach based on the extrapolation of clinical data, we predicted that the concentration of 32 of these neuroactive pharmaceuticals in surface waters in England may be high enough to elicit pharmacological effects in wild fish. The ecotoxicological effects of the vast majority of these compounds are currently uncharacterised. Overall, these results highlight the importance of addressing this environmental challenge from a mixture toxicology and systems perspective. The knowledge platform developed in the present study can guide future region-specific prioritisation efforts, inform the design of mixture studies, and foster interdisciplinary efforts aimed at identifying novel approaches to predict and interpret the ecological implications of chemical-induced behaviour disruption.
Risk Assessment of Dietary Exposure to Organophosphorus Flame Retardants in Children by Using HBM-Data
Authors: Plichta Veronika , Steinwider Johann , Vogel Nina , Weber Till , Kolossa-Gehring Marike , Murínová Lubica Palkovičová , Wimmerová Soňa , Tratnik Janja Snoj , Horvat Milena , Koppen Gudrun , Govarts Eva , Gilles Liese , Rodriguez Martin Laura , Schoeters Greet , Covaci Adrian , Fillol Clémence , Rambaud Loïc , Jensen Tina Kold , Rauscher-Gabernig Elke
Published: May 2022

Due to their extensive usage, organophosphorus flame retardants (OPFRs) have been detected in humans and in the environment. Human are exposed to OPFRs via inhalation of indoor air, dust uptake or dietary uptake through contaminated food and drinking water. Only recently, few studies addressing dietary exposure to OPFRs were published. In this study, we used human biomonitoring (HBM) data of OPFRs to estimate how much the dietary intake may contribute to the total exposure. We estimated by reverse dosimetry, the daily intake of tris (2-chloroethyl) phosphate (TCEP), tris (1-chloro-2-propyl) phosphate (TCIPP), tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) for children using HBM data from studies with sampling sites in Belgium, Denmark, France, Germany, Slovenia and Slovakia. For estimating the dietary exposure, a deterministic approach was chosen. The occurrence data of selected food categories were used from a published Belgium food basket study. Since the occurrence data were left-censored, the Lower bound (LB)—Upper bound (UB) approach was used. The estimated daily intake (EDI) calculated on the basis of urine metabolite concentrations ranged from 0.03 to 0.18 µg/kg bw/d for TDCIPP, from 0.05 to 0.17 µg/kg bw/d for TCIPP and from 0.02 to 0.2 µg/kg bw/d for TCEP. Based on national food consumption data and occurrence data, the estimated dietary intake for TDCIPP ranged from 0.005 to 0.09 µg/kg bw/d, for TCIPP ranged from 0.037 to 0.2 µg/kg bw/d and for TCEP ranged from 0.007 to 0.018 µg/kg bw/d (summarized for all countries). The estimated dietary intake of TDCIPP contributes 11–173% to the EDI, depending on country and LB-UB scenario. The estimated dietary uptake of TCIPP was in all calculations, except in Belgium and France, above 100%. In the case of TCEP, it is assumed that the dietary intake ranges from 6 to 57%. The EDI and the estimated dietary intake contribute less than 3% to the reference dose (RfD). Therefore, the estimated exposure to OPFRs indicates a minimal health risk based on the current knowledge of available exposure, kinetic and toxicity data. We were able to show that the dietary exposure can have an impact on the general exposure based on our underlying exposure scenarios.
Updated Pathways in Cardiorenal Continuum after Kidney Transplantation
Authors: Laučytė-Cibulskienė Agnė , Biglarnia Ali-Reza , Wallquist Carin , Christensson Anders
Published: May 2022

Cardiovascular disease (CVD) remains one of the leading causes for increased morbidity and mortality in chronic kidney disease (CKD). Kidney transplantation is the preferred treatment option for CKD G5. Improved perioperative and postoperative care, personalized immunosuppressive regimes, and refined matching procedures of kidney transplants improves cardiovascular health in the early posttransplant period. However, the long-term burden of CVD is considerable. Previously underrecognized, the role of the complement system alongside innate immunity, inflammaging, structural changes in the glomerular filtration barrier and early vascular ageing also seem to play an important role in the posttransplant management. This review provides up-to-date knowledge on these pathways that may influence the cardiovascular and renal continuum and identifies potential targets for future therapies. Arterial destiffening strategies and the applicability of sodium-glucose cotransporter 2 inhibitors and their role in cardiovascular health after kidney transplantation are also addressed.
Risk Factors for Groin Pain in Male High School Soccer Players Undergoing an Injury Prevention Program: A Cluster Randomized Controlled Trial
Authors: Fujisaki Kazuki , Akasaka Kiyokazu , Otsudo Takahiro , Hattori Hiroshi , Hasebe Yuki , Hall Toby
Published: May 2022

Little is known about the risk factors for developing groin pain in high school soccer players. Therefore, the purpose of the study is to investigate the risk factors for developing inguinal pain in high school soccer players who are undergoing an injury prevention program. A cluster randomized controlled trial was conducted on 202 high school soccer players. Players were allocated to either group A (3 schools, 66 players) receiving the Copenhagen adduction exercise (CAE) alone, or group B (2 schools, 73 players) receiving the CAE and Nordic hamstrings exercise, or group C, the control group without any intervention (2 schools, 63 players). Hip range of motion (ROM) and strength measures were assessed prior to a groin injury prevention program and used in univariate and multivariate analysis to predict development of groin pain. Logistic regression analysis identified that hip abduction ROM and eccentric adductor strength of the dominant leg were factors in the development of groin pain. Increased abduction ROM and decreased eccentric adductor muscle strength of the dominant leg were risk factors for the development of groin pain.
Penetrating Cardiac Injuries: Outcome of Treatment from a Level 1 Trauma Centre in South Africa
Authors: Makhadi Shumani , Moeng Maeyane Stephens , Ede Chikwendu , Jassat Farhana , Palweni Sechaba Thabo
Published: May 2022

Background: Penetrating cardiac injuries are rare in South African and international literature. Penetrating cardiac injuries are regarded as one of the most lethal injuries in trauma patients. The mechanism of injury varies across the world. In developing countries, stab wounds cause the majority of penetrating cardiac injuries. These injuries remain clinically challenging and are associated with high mortalities. Aim: To describe our experience with penetrating cardiac injuries and the outcome of their management at a level 1 trauma unit in Johannesburg, South Africa. Materials and methods: We retrospectively reviewed all patients who presented with penetrating cardiac injuries over a period of four years (1 January 2016 to 31 December 2019). The patients were identified using the hospital database. The patient’s demographics, mechanism of injury, injury severity score, vital signs, investigation findings, final diagnosis, type of operation, length of hospital stay, morbidities, and mortalities were recorded. Results: There was a total of 167 patients with penetrating cardiac injuries identified. There were 151 (90.4%) males, with an overall median age of 29 years (IQR 24–34). Stab wounds accounted for 77.8% of the injuries, while gunshot wounds (GSW) accounted for 22.2%. The median injury severity score (ISS) and revised trauma score (RTS) were 25 and 7.1, respectively. The right ventricle was the most injured chamber (34.7%), followed by the left ventricle (29.3%), right auricle (13.2%), right atrium (10.2%), and combined injuries accounted for 7% of injuries. A commonly used incision was a sternotomy (51.5%), left anterior-lateral thoracotomy (26.9%), emergency room thoracotomy (19.2%), and clamshell thoracotomy (2.4%). The overall mortality rate was 40.7%, with a 29.2% mortality in the stab wounds. Twenty-four (14.4%) patients died in the emergency department, sixteen (9.6%) patients died on the table in theatre, and the remaining twenty-eight (16.7%) died in the intensive care unit or wards. Gunshot wounds, other associated injuries, right ventricle injuries, a high ISS, low RTS, and low Glasgow coma scale were all significantly more likely to result in death (p < 0.001). Conclusions: Penetrating cardiac injuries are often fatal, but the mortality can be improved with appropriate resuscitation and a work-up. The injuries to the heart can be safely managed by trauma/general surgeons in our setting. The physiology in presentation and other associated injuries determines outcomes in patients with penetrating cardiac injury.
Effective Actions for Regge Piecewise Flat Quantum Gravity
Authors: Miković Aleksandar
Published: May 2022

We review the construction of the path integral and the corresponding effective action for the Regge formulation of General Relativity under the assumption that the short-distance structure of the spacetime is not a smooth 4-manifold, but a piecewise linear manifold based on a triangulation of a smooth 4-manifold. We point out that the exponentially damped 4-volume path-integral measure does not give a finite path integral, although it can be used for the construction of the perturbative effective action. We modify the 4-volume measure by multiplying it by an inverse power of the product of the edge-lengths such that the new measure gives a finite path integral while it retains all the nice features of the unmodified measure.
Understanding the Urban Middle-Class and Its Housing Characteristics—Case Study of Casablanca, Morocco
Authors: Mharzi Alaoui Hicham , Radoine Hassan , Chenal Jérôme , Yakubu Hassan , Bajja Salwa
Published: May 2022

With the rapid urbanization occurring across African cities, the emergence of a middle class is exerting its influence on the urban form and structure. Matching their social status and drawing on global influences, the housing characteristics of this class are distinctive in spatial organization, material choice, and location, among others. Understanding these emerging typologies is critical for urban housing policy to be responsive to the needs and preferences of this class. The present paper aims first to develop a new approach for defining the middle class in the African context and second, to analyze its housing typology through a multidimensional analysis based on housing attributes and socio-economic characteristics in Casablanca City. A data-driven approach based on principal component analysis (PCA) has been used to define multidimensionally the middle class and its housing typology. Through the construction of a multidimensional composite index to measure middle class, the study highlighted that middle-class housing is characterized by an adequate condition, a suitable size (two to three rooms), and affordability between 8000 and 11,000 Moroccan dirhams (MAD) per square meter. In addition, although the Moroccan modern houses (two-floor single-family housing) and apartments (four floors or greater) are the most occupied by the middle class, the study showed that as income and social mobility increase, the apartment building and the villa are increasingly preferred to the Moroccan modern house.
The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1
Authors: Davis Carly M. , Ruest Marta K. , Cole Jamie H. , Dennis Jonathan J.
Published: May 2022

Bacteriophage JC1 is a Podoviridae phage with a C1 morphotype, isolated on host strain Burkholderia cenocepacia Van1. Phage JC1 is capable of infecting an expansive range of Burkholderia cepacia complex (Bcc) species. The JC1 genome exhibits significant similarity and synteny to Bcep22-like phages and to many Ralstonia phages. The genome of JC1 was determined to be 61,182 bp in length with a 65.4% G + C content and is predicted to encode 76 proteins and 1 tRNA gene. Unlike the other Lessieviruses, JC1 encodes a putative helicase gene in its replication module, and it is in a unique organization not found in previously analyzed phages. The JC1 genome also harbours 3 interesting moron genes, that encode a carbon storage regulator (CsrA), an N-acetyltransferase, and a phosphoadenosine phosphosulfate (PAPS) reductase. JC1 can stably lysogenize its host Van1 and integrates into the 5′ end of the gene rimO. This is the first account of stable integration identified for Bcep22-like phages. JC1 has a higher global virulence index at 37 °C than at 30 °C (0.8 and 0.21, respectively); however, infection efficiency and lysogen stability are not affected by a change in temperature, and no observable temperature-sensitive switch between lytic and lysogenic lifestyle appears to exist. Although JC1 can stably lysogenize its host, it possesses some desirable characteristics for use in phage therapy. Phage JC1 has a broad host range and requires the inner core of the bacterial LPS for infection. Bacteria that mutate to evade infection by JC1 may develop a fitness disadvantage as seen in previously characterized LPS mutants lacking inner core.
Genomic and In Vitro Phenotypic Comparisons of Epidemic and Non-Epidemic Getah Virus Strains
Authors: Mohamed-Romai-Noor Noor-Adila , Sam Sing-Sin , Teoh Boon-Teong , Hamim Zur-Raiha , AbuBakar Sazaly
Published: May 2022

Getah virus is an emerging mosquito-borne animal pathogen. Four phylogenetic groups of GETV, Group I (GI), GII, GIII and GIV, were identified. However, only the GETV GIII was associated with disease epidemics suggesting possible virulence difference in this virus group. Here, we compared the genetic and in vitro phenotypic characteristics between the epidemic and non-epidemic GETV. Our complete coding genome sequence analyses revealed several amino acid substitutions unique to the GETV GIII and GIV groups, which were found mainly in the hypervariable domain of nsP3 and E2 proteins. Replication kinetics of the epidemic (GIII MI-110 and GIII 14-I-605) and non-epidemic GETV strains (prototype GI MM2021 and GIV B254) were compared in mammalian Vero cells and mosquito C6/36 and U4.4 cells. In all cells used, both epidemic GETV GIII MI-110 and GIII 14-I-605 strains showed replication rates and mean maximum titers at least 2.7-fold and 2.3-fold higher than those of GIV B254, respectively (Bonferroni posttest, p < 0.01). In Vero cells, the epidemic GETV strains caused more pronounced cytopathic effects in comparison to the GIV B254. Our findings suggest that higher virus replication competency that produces higher virus titers during infection may be the main determinant of virulence and epidemic potential of GETV.
A Novel Sandwich ELASA Based on Aptamer for Detection of Largemouth Bass Virus (LMBV)
Authors: Zhang Xinyue , Zhang Zemiao , Li Junrong , Huang Xiaohong , Wei Jingguang , Yang Jiahui , Guan Lingfeng , Wen Xiaozhi , Wang Shaowen , Qin Qiwei
Published: May 2022

Largemouth bass virus (LMBV) is a major viral pathogen in largemouth bass culture, usually causing high mortality and heavy economic losses. Accurate and early detection of LMBV is crucial for diagnosis and control of the diseases caused by LMBV. Previously, we selected the specific aptamers, LA38 and LA13, targeting LMBV by systematic evolution of ligands by exponential enrichment (SELEX). In this study, we further generated truncated LA38 and LA13 (named as LA38s and LA13s) with high specificity and affinities and developed an aptamer-based sandwich enzyme-linked apta-sorbent assay (ELASA) for LMBV diagnosis. The sandwich ELASA showed high specificity and sensitivity for the LMBV detection, without cross reaction with other viruses. The detection limit of the ELASA was as low as 1.25 × 102 LMBV-infected cells, and the incubation time of the lysate and biotin labeled aptamer was as short as 10 min. The ELASA could still detect LMBV infection in spleen lysates at dilutions of 1/25, with good consistency of qRT-PCR. For the fish samples collected from the field, the sensitivity of ELASA was 13.3% less than PCR, but the ELASA was much more convenient and less time consuming. The procedure of ELASA mainly requires washing and incubation, with completion in approximately 4 h. The sandwich ELASA offers a useful tool to rapidly detect LMBV rapidly, contributing to control and prevention of LMBV infection.
Role of Microbiota in Viral Infections and Pathological Progression
Authors: Mizutani Taketoshi , Ishizaka Aya , Koga Michiko , Tsutsumi Takeya , Yotsuyanagi Hiroshi
Published: May 2022

Viral infections are influenced by various microorganisms in the environment surrounding the target tissue, and the correlation between the type and balance of commensal microbiota is the key to establishment of the infection and pathogenicity. Some commensal microorganisms are known to resist or promote viral infection, while others are involved in pathogenicity. It is also becoming evident that the profile of the commensal microbiota under normal conditions influences the progression of viral diseases. Thus, to understand the pathogenesis underlying viral infections, it is important to elucidate the interactions among viruses, target tissues, and the surrounding environment, including the commensal microbiota, which should have different relationships with each virus. In this review, we outline the role of microorganisms in viral infections. Particularly, we focus on gaining an in-depth understanding of the correlations among viral infections, target tissues, and the surrounding environment, including the commensal microbiota and the gut virome, and discussing the impact of changes in the microbiota (dysbiosis) on the pathological progression of viral infections.
Seroprevalence of SARS-CoV-2 IgG Antibodies and Factors Associated with SARS-CoV-2 IgG Neutralizing Activity among Primary Health Care Workers 6 Months after Vaccination Rollout in France
Authors: Decarreaux Dorine , Pouquet Marie , Souty Cecile , Vilcu Ana-Maria , Prévot-Monsacre Pol , Fourié Toscane , Villarroel Paola Mariela Saba , Priet Stephane , Blanché Hélène , Sebaoun Jean-Marc , Deleuze Jean-François , Turbelin Clément , Werner Andréas , Kochert Fabienne , Grosgogeat Brigitte , Rabiega Pascaline , Laupie Julien , Abraham Nathalie , Guerrisi Caroline , Noël Harold , Van der Werf Sylvie , Carrat Fabrice , Hanslik Thomas , Charrel Remi , De Lamballerie Xavier , Blanchon Thierry , Falchi Alessandra
Published: May 2022

We aimed to investigate the immunoglobulin G response and neutralizing activity against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) among primary health care workers (PHCW) in France and assess the association between the neutralizing activity and several factors, including the coronavirus disease 2019 (COVID-19) vaccination scheme. A cross-sectional survey was conducted between 10 May 2021 and 31 August 2021. Participants underwent capillary blood sampling and completed a questionnaire. Sera were tested for the presence of antibodies against the nucleocapsid (N) protein and the S-1 portion of the spike (S) protein and neutralizing antibodies. In total, 1612 PHCW were included. The overall seroprevalences were: 23.6% (95% confidence interval (CI) 21.6–25.7%) for antibodies against the N protein, 94.7% (93.6–95.7%) for antibodies against the S protein, and 81.3% (79.4–83.2%) for neutralizing antibodies. Multivariate regression analyses showed that detection of neutralizing antibodies was significantly more likely in PHCW with previous SARS-CoV-2 infection than in those with no such history among the unvaccinated (odds ratio (OR) 16.57, 95% CI 5.96–59.36) and those vaccinated with one vaccine dose (OR 41.66, 95% CI 16.05–120.78). Among PHCW vaccinated with two vaccine doses, the detection of neutralizing antibodies was not significantly associated with previous SARS-CoV-2 infection (OR 1.31, 95% CI 0.86–2.07), but was more likely in those that received their second vaccine dose within the three months before study entry than in those vaccinated more than three months earlier (OR 5.28, 95% CI 3.51–8.23). This study highlights that previous SARS-CoV-2 infection and the time since vaccination should be considered when planning booster doses and the design of COVID-19 vaccine strategies.
Antiviral Drug Discovery for the Treatment of COVID-19 Infections
Authors: Ng Teresa I. , Correia Ivan , Seagal Jane , DeGoey David A. , Schrimpf Michael R. , Hardee David J. , Noey Elizabeth L. , Kati Warren M.
Published: May 2022

The coronavirus disease 2019 (COVID-19) pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a recently emerged human coronavirus. COVID-19 vaccines have proven to be successful in protecting the vaccinated from infection, reducing the severity of disease, and deterring the transmission of infection. However, COVID-19 vaccination faces many challenges, such as the decline in vaccine-induced immunity over time, and the decrease in potency against some SARS-CoV-2 variants including the recently emerged Omicron variant, resulting in breakthrough infections. The challenges that COVID-19 vaccination is facing highlight the importance of the discovery of antivirals to serve as another means to tackle the pandemic. To date, neutralizing antibodies that block viral entry by targeting the viral spike protein make up the largest class of antivirals that has received US FDA emergency use authorization (EUA) for COVID-19 treatment. In addition to the spike protein, other key targets for the discovery of direct-acting antivirals include viral enzymes that are essential for SARS-CoV-2 replication, such as RNA-dependent RNA polymerase and proteases, as judged by US FDA approval for remdesivir, and EUA for Paxlovid (nirmatrelvir + ritonavir) for treating COVID-19 infections. This review presents an overview of the current status and future direction of antiviral drug discovery for treating SARS-CoV-2 infections, covering important antiviral targets such as the viral spike protein, non-structural protein (nsp) 3 papain-like protease, nsp5 main protease, and the nsp12/nsp7/nsp8 RNA-dependent RNA polymerase complex.
Cell Lines for the Development of African Swine Fever Virus Vaccine Candidates: An Update
Authors: Meloni Dionigia , Franzoni Giulia , Oggiano Annalisa
Published: May 2022

African swine fever virus (ASFV) is the etiological agent of a highly lethal disease in both domestic and wild pigs. The virus has rapidly spread worldwide and has no available licensed vaccine. An obstacle to the construction of a safe and efficient vaccine is the lack of a suitable cell line for ASFV isolation and propagation. Macrophages are the main targets for ASFV, and they have been widely used to study virus–host interactions; nevertheless, obtaining these cells is time-consuming and expensive, and they are not ethically suitable for the production of large-scale vaccines. To overcome these issues, different virulent field isolates have been adapted on monkey or human continuous cells lines; however, several culture passages often lead to significant genetic modifications and the loss of immunogenicity of the adapted strain. Thus, several groups have attempted to establish a porcine cell line able to sustain ASFV growth. Preliminary data suggested that some porcine continuous cell lines might be an alternative to primary macrophages for ASFV research and for large-scale vaccine production, although further studies are still needed. In this review, we summarize the research to investigate the most suitable cell line for ASFV isolation and propagation.
Complete (Humoral and Cellular) Response to Vaccination against COVID-19 in a Group of Healthcare Workers-Assessment of Factors Affecting Immunogenicity
Authors: Morgiel Ewa , Szmyrka Magdalena , Madej Marta , Sebastian Agata , Sokolik Renata , Andrasiak Iga , Chodyra Maria , Walas-Antoszek Małgorzata , Korman Lucyna , Świerkot Jerzy
Published: May 2022

Vaccination is the best way to limit the extent of the COVID pandemic. Knowledge of the duration of the immune response will allow the planning of a vaccination protocol. This study aims to validate the complete (humoral and cellular) immune responses over time in large population groups following the full vaccination of healthcare professionals in real-life conditions and to assess the relationship between antibody levels and T-cell activity in relation to the characteristics of the study group. The samples for the study were obtained from volunteers (staff of two hospitals) on three occasions: before vaccination, T0, then 4–9 weeks after full vaccination (two doses BNT162b2), T1, and 7–9 months after vaccination, T2. The humoral response was investigated by the titre of anti-SARS-CoV-2 IgG antibodies to S1 protein. Assays were performed three times at intervals. The cellular response was assessed in a subgroup of 189 subjects by QuanT-Cell SARS-CoV-2 (IGRA). The assay was performed once. A group of 344 subjects fully vaccinated with the BNT162b2 vaccine were included in the study. The humoral response was observed in 100% of subjects at both 4–7 weeks and 7–9 months, but antibody titres fell by almost 90% in this interval. The cellular response was observed in 94% (177/189) of subjects 7–9 months after the second dose of vaccine. In subjects with a negative cellular response, eight out of 12 smoked. A factor associated with greater immunogenicity of vaccination was past SARS-CoV-2 infection. The administration of full BNT162b2 vaccination (two doses) induces humoral and cellular responses detectable even more than six months after vaccination. Smoking may be a factor associated with impaired cellular response to vaccination.
Mosquirix™ RTS, S/AS01 Vaccine Development, Immunogenicity, and Efficacy
Authors: Nadeem Aroosa Younis , Shehzad Adeeb , Islam Salman Ul , Al-Suhaimi Ebtesam A. , Lee Young Sup
Published: May 2022

Malaria is a parasitic infection caused by bites from Plasmodium falciparum (P. falciparum)-infected mosquitoes with a present scale of symptoms ranging from moderate fever to neurological disorders. P. falciparum is the most lethal of the five strains of malaria, and is a major case of morbidity and mortality in endemic regions. Recent advancements in malaria diagnostic tools and prevention strategies have improved conjugation antimalarial therapies using fumigation and long-lasting insecticidal sprays, thus lowering malarial infections. Declines in the total number of infected individuals have been correlated with antimalarial drugs. Despite this, malaria remains a major health threat, affecting more than 30 million men, women, and children around the globe, and 20 percent of all children around the globe have malaria parasites in their blood. To overcome this life-threatening condition, novel therapeutic strategies, including immunization, are urgently needed to tackle this infection around the world. In line with this, the development of the RTS, S vaccine was a significant step forward in the fight against malaria. RTS, S is a vaccine for P. falciparum in which R specifies central repeat units, T the T-cell epitopes, and S indicates surface antigen. The RTS, S/AS01 malarial vaccine was synthesized and screened in several clinical trials between 2009 and 2014, involving thousands of young children in seven African countries, showing that children who received the vaccine did not suffer from severe malaria. Mosquirix™ was approved by the World Health Organization in 2021, indicating it to be safe and advocating its integration into routine immunization programs and existing malaria control measures. This paper examines the various stages of the vaccine’s development, including the evaluation of its immunogenicity and efficacy on the basis of a total of 2.3 million administered doses through a routine immunization program. The protection and effectiveness provided by the vaccine are strong, and evidence shows that it can be effectively delivered through the routine child immunization platform. The economic cost of the vaccine remains to be considered.
Influenza Viruses and Vaccines: The Role of Vaccine Effectiveness Studies for Evaluation of the Benefits of Influenza Vaccines
Authors: Trombetta Claudia Maria , Kistner Otfried , Montomoli Emanuele , Viviani Simonetta , Marchi Serena
Published: May 2022

Influenza is a vaccine preventable disease and vaccination remains the most effective method of controlling the morbidity and mortality of seasonal influenza, especially with respect to risk groups. To date, three types of influenza vaccines have been licensed: inactivated, live-attenuated, and recombinant haemagglutinin vaccines. Effectiveness studies allow an assessment of the positive effects of influenza vaccines in the field. The effectiveness of current influenza is suboptimal, being estimated as 40% to 60% when the vaccines strains are antigenically well-matched with the circulating viruses. This review focuses on influenza viruses and vaccines and the role of vaccine effectiveness studies for evaluating the benefits of influenza vaccines. Overall, influenza vaccines are effective against morbidity and mortality in all age and risk groups, especially in young children and older adults. However, the effectiveness is dependent on several factors such as the age of vaccinees, the match between the strain included in the vaccine composition and the circulating virus, egg-adaptations occurring during the production process, and the subject’s history of previous vaccination.
Poloxamer-188 Adjuvant Efficiently Maintains Adaptive Immunity of SARS-CoV-2 RBD Subunit Vaccination through Repressing p38MAPK Signaling
Authors: Chen Chao-Hung , Lin Yu-Jen , Cheng Li-Ting , Lin Chien-Hung , Ke Guan-Ming
Published: May 2022

Poloxamer-188 (P188) is a nonionic triblock linear copolymer that can be used as a pharmaceutical excipient because of its amphiphilic nature. This study investigated whether P188 can act as an adjuvant to improve the immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor binding domain (RBD) subunit vaccine. BALB/c mice were vaccinated twice with the RBD antigen alone or in combination with P188 or MF59 (a commercial adjuvant for comparison purposes). The resulting humoral and cellular immunity were assessed. Results showed that P188 helped elicit higher neutralizing activity than MF59 after vaccination. P188 induced significant humoral immune response, along with type 1 T helper (Th1) and type 2 T helper (Th2) cellular immune response when compared with MF59 due to repressing p38MAPK phosphorylation. Furthermore, P188 did not result in adverse effects such as fibrosis of liver or kidney after vaccination. In conclusion, P188 is a novel adjuvant that may be used for safe and effective immune enhancement of the SARS-CoV-2 RBD antigen.
A Way Forward for Electric Vehicle in Greater Bay Area: Challenges and Opportunities for the 21st Century
Authors: Lau Yui-Yip , Wu Andrew Yang , Yan Mak Wing
Published: May 2022

The Greater Bay Area (GBA) accounts for a high percentage of pollution due to the large number of internal combustion engines. In the past few decades, there has been a significant increase in internal combustion engines vehicles while electric vehicles have not taken off yet in GBA. To a certain extent, the acceptance of electric vehicles is still questionable from the industrial practitioners and local communities. As such, this research study aims to identify the challenges and opportunities of electric vehicles in GBA to address the future direction of electric vehicles in GBA. In this study, it identifies technology and economy as the main driving forces behind the development of electric vehicles. Furthermore, sustainability, safety, and the life of the batteries may induce the slow adoption of electric vehicles. As expected, the study develops a research agenda and contributes new knowledge in the field of electric vehicle.