bluuebunny/crossref_metadata_2025 huggingface dataset:
Copper Ore Identification using Spectral Similarity Measurement from Hyperion Image, Mapping of Porphyry Copper Mineralized Zone
Authors: Banerjee Krishnendu , Jain Manish Kumar
Published: February 2018

This work illustrates the effectiveness of hyperspectal image spectroscopy and lab spectroscopy in identiûcation techniques of minerals in alteration zone of ore body. The adopted procedure involves testing of Hyperion image spectra, their processing for noise, spectral matching and spectral similarity measurement with selected library spectra. Average weighted spectral similarity; visual and statistical matching techniques were used to select end-members from image spectra. Minimum noise fraction and pixel purity index technique were used to retrieve end-member spectra from hyperion image. Hyperspectral image like hyperion has the capability to deliver laboratory standard spectroscopic result. This paper illustrates the capability of hyperion image spectra in copper ore identification and mapping of chalcopyrite outcrop. A systematic approach has been made in this paper. This approach describes how image end member spectra and laboratory spectra can be co-related to fetch accurate spectral form of chalcopyrite ores. Thus, statistical and graphical comparison has been carried out between image derived end member spectra and laboratory spectra of chalcopyrite for better accuracy. The visual measurements is satisfactory, R = 0.973 for fine and 0.976 for medium grained chalcopyrite ore. Excellent statistical signiûcance levels (90–97%) are found while comparing these spectra. There are many success stories of sub-pixel and N-dimensional feature space methods to separate the hydrothermal alteration zones from iron oxide mixed ore bodies. Thus, unmixing methods are very useful for mapping of most dominating mineral parts of a pixel from hyperspectral images which have coarse spatial resolution. Finally, mapping of mineralized zone has been achieved through sub-pixel based classifiers like spectral angle mapper (SAM), constrained energy minimization (CEM) and adaptive coherence estimator (ACE) techniques.
Lexical verbs in a medical case-report wordlist
Authors: Canziani Tatiana , Mungra Philippa
Published: February 2018

Clinical case reports or clinical cases (CRs) are, perhaps, the most widely read text type in medicine, since they contain a detailed description of the patient’s medical history and symptoms and thus furnish ample teaching material for physicians-in-training. For non-native speakers of English in medicine, autonomous learning is often restricted because of a lack of medical lexicon, poor academic vocabulary, and weak lexical verb use. Here, we present the results of an investigation of lexical verbs: their distribution, classification, and contextual use in the different sections of the genre CRs. We suggest that lexical verbs with contextual use should be included in medical dictionaries to aid vocabulary development for all levels of English language competence. We found that relational and reportingverbs predominate in CRs and are used to describe and contextualize author observations. Stative verbs are generally found to describe patient data, while change of state verbs generally refers to patient response to therapy. Contextual analysis suggests that lexical verbs categories might be related to the moves of this genre, useful for teaching the structure of medical publications. We give some applications of this investigation to dictionary building and in integrating corpora in teaching and eventually in testing activities.
Proximal Hamstring Avulsion Injury in an Ultimate Frisbee Player: Case Report and Literature Review
Authors: Suero Eduardo M. , Mohamed Omar , Mustafa Citak , Musa Citak , Aaron Daluiski , Pearle Andrew D.
Published: February 2018

There are few studies describing the occurrence of injuries in ultimate—commonly known as Ultimate Frisbee. We present the case of an elite ultimate player who suffered a proximal hamstring avulsion injury. A 26-year-old male athlete was evaluated in our office 2 weeks after being involved in a collision during an ultimate game. Magnetic resonance imaging of the hamstrings showed an extensive, high-grade right-sided tear with a 5–6 cm of tendon retraction and moderate signal in the muscle extending to the thigh. Delayed open repair of the avulsed tendon was performed through a posterior approach under general anaesthesia. Twelve months after surgery, the patient had no complaints, and his range of motion and strength had recovered to preinjury levels. The patient has since competed at the national club level and the professional level. This case reports on the success of the primary repair even after a delay in treatment of several weeks and significant tendon retraction.
Exploring Regional Patterns of Agritourism in the U.S.: What's Driving Clusters of Enterprises?
Authors: Van Sandt Anders , Low Sarah A. , Thilmany Dawn
Published: February 2018

Agritourism is a consumer-driven innovation that producers are exploring as a means to diversify and grow farm-based revenues. In order to help guide management and policy decisions, we conduct an exploratory spatial data analysis and find that travel infrastructure, region and rurality, characteristics of the local economy, and proximity to outdoor attractions are all significantly associated with the probability of a county being an agritourism hot spot. Mapping our primary spatial analysis’ residuals, we further identify counties with unique agritourism market conditions as a starting point to identify best practices that other regions interested in agritourism development might follow.
What Kenyan Youth Want and Why It Matters for Peace
Authors: King Elisabeth
Published: February 2018

The international aid community presents education and employment programs as the keys to mitigating youth participation in violence. Yet, existing evidence suggests that faith in such programs may be misplaced. This study investigates this disconnect between faith and evidence. It argues that education and employment programs are commonly built on an economically-focused “dominant discourse” that makes presumptions about youth and their interests. Based on qualitative research with youth in Nairobi, Kenya, it further argues that this dominant discourse overlooks self-identity and social connectedness factors that are crucial to youth, as well as the limitations imposed by governance and structural conditions.
The legislative representation of public opinion policy priorities in Italy
Authors: Visconti Francesco
Published: February 2018

Normative democratic theory requires political actors in parliament and government to represent not only the citizens’ policy preferences, but also their issue priorities. This article investigates Italian dynamic agenda representation – the transmission of public priorities into the policy priorities of the Italian political system. To assess the public’s policy priorities, data on the Most Important Problem from the Eurobarometer polls are used, while the legislative agendas of the members of parliament (MPs) and government are built following the rules of the Comparative Agendas Project. The results of longitudinal analyses across 10 policy areas and 20 semesters (2003–13) suggest a persistent link between the public’s agenda and the prioritization of legislation by the Italian parliament, majority MPs, and government. Contrary to expectations, the opposition does not seem to be responsive to public opinion policy problems when introducing bills.
First fossil crabs (Crustacea, Decapoda, Brachyura) from the Kerguelen Islands (Miocene) with exceptionally preserved gills
Authors: Robin Ninon , Van Bakel Barry W.M. , Blanc-Valleron Marie-Madeleine , Noël Pierre Y. , Lemaire Laurent , Charbonnier Sylvain
Published: February 2018

Kerguelen Islands are predominantly volcanic lands, thus fossil references are very uncommon. However, its Miocene fossils are of specific interest for understanding migration routes of some taxa during the Cenozoic, given the intermediary position of Kerguelen with various continents. Despite this fossil rarity, we studied herein hundreds of nodules corresponding to the sole known fossil brachyurans (and unique Decapoda) from Kerguelen both for their systematics and their preservation. Indeed, these crabs display some internal fragile structures that are rarely fossilized, such as the gills’ branchial lamellae, preserved in volume. The preservation of these gills and their diagenetic features were documented through traditional imagery (SEM), including morphological comparison to modern gills, and with petrographic and geochemical analyses (EDS, X-ray diffractometry). Some cheilostome bryozoans were observed as probable foulers of the crabs carcasses. The fossil material corresponds to a new cancrid crab (Romaleon franciscaen. sp.) and its occurrence may imply a novel route from South America westward for the geographic migration of the genusRomaleon, since its Cretaceous emergence. The cup-shape and the number and the organization of the gills in these fossil Cancridae specimens are similar to those observed in their extant representatives. Gill preservation in 3D is linked to very early phosphatization of the system during diagenesis, as shown by the nodule matrix, and likely to deposition of a thin clayey cover on the soft-tissues. The implication of intrinsic phosphorous in the differential phosphatization of the crabs’ anatomy remains difficult to determine.
Is There Convergence in National Alcohol Consumption Patterns? Evidence from a Compositional Time Series Approach
Authors: Mills Terence C.
Published: February 2018

Holmes and Anderson (2017a) introduce two extensive data sets on world alcohol consumption and expenditure and with them investigate, among other things, the possible convergence of national alcohol consumption patterns using wine, beer, and spirit shares. Such share data define a composition, on which conventional statistical analysis using covariances and correlations is invalid. This note reanalyses the data using techniques appropriate for a composition and introduces a statistic that can validly track the variation in national shares around the global mean through time. This variability statistic shows that such convergence of national alcohol patterns has clearly taken place over the period 1961 to 2014 and thus confirms Holmes and Anderson's findings using a valid statistical approach. (JEL Classifications: C18, D12, L66)
EFFECTS OF FERTILIZATION ON POREWATER NUTRIENTS, GREENHOUSE-GAS EMISSIONS AND RICE PRODUCTIVITY IN A SUBTROPICAL PADDY FIELD
Authors: WANG WEIQI , SARDANS JORDI , WANG CHUN , TONG CHUAN , JI QINYANG , PEÑUELAS JOSEP
Published: February 2018

Suitable fertilization is crucial for the sustainability of rice production and for the potential mitigation of global warming. The effects of fertilization on porewater nutrients and greenhouse-gas fluxes in cropland, however, remain poorly known. We studied the effects of no fertilization (control), standard fertilization and double fertilization on the concentrations of porewater nutrients, greenhouse-gas fluxes and emissions, and rice yield in a subtropical paddy in southeastern China. Double fertilization increased dissolved NH4+in porewater. Mean CO2and CH4emissions were 13.5% and 7.4%, and 20.4% and 39.5% higher for the standard and double fertilizations, respectively, than the control. N2O depositions in soils were 61% and 101% higher for the standard and double fertilizations, respectively, than the control. The total global warming potentials (GWPs) for all emissions were 14.1% and 10.8% higher for the standard and double fertilizations, respectively than the control, with increasing contribution of CH4with fertilization and a CO2contribution > 85%. The total GWPs per unit yield were significantly higher for the standard and double fertilizations than the control by 7.3% and 10.9%, respectively. The two levels of fertilization did not significantly increase rice yield. Prior long-term fertilization in the paddy (about 20 years with annual doses of 95 kg N ha−1, 70 kg P2O5ha−1and 70 kg K2O ha−1) might have prevented these fertilizations from increasing the yield. However, fertilizations increased greenhouse-gas emissions. This situation is common in paddy fields in subtropical China, suggesting a saturation of soil nutrients and the necessity to review current fertilization management. These areas likely suffer from unnecessary nutrient leaching and excessive greenhouse-gas emissions. These results provide a scientific basis for continued research to identify an easy and optimal fertilization management solution.
English spelling: Adding /ʃǝn/ (or /ʒǝn/) to base-words and changing from -tion to -sion
Authors: Achiri–Taboh Blasius
Published: February 2018

One tricky problem with English spelling is the variation in the nominalization suffix often represented in discourse as ‘shun’, mainly between -tion and -sion. Current ELT textbooks have generally not discussed rules for its spelling. However, following online resources, some basic rules are in current debate, with two main schools of thought, each falling in line with one of two approaches that can be called the ‘word-based model’ and the ‘base-word model’. In this article, I show the base-word model to be preferred, determine the actual suffix and its underlying form, and elaborate on base-word ending clues to yield a general synchronic rule for changing from -tion to -sion, albeit with exceptions.
Transmission of respiratory and gastrointestinal infections in German households with children attending child care
Authors: Schlinkmann K. M. , Bakuli A. , Karch A. , Meyer F. , Dreesman J. , Monazahian M. , Mikolajczyk R.
Published: February 2018

Transmission of acute respiratory infections (ARI) and acute gastroenteritis (AGE) often occurs in households. The aim of this study was to assess which proportion of ARI and AGE is introduced and transmitted by children in German households with children attending child care. We recruited families with children aged 0–6 years in Braunschweig (Germany), for a 4 months prospective cohort study in the winter period 2014/2015. Every household member was included in a health diary and used nasal swabs for pathogen identification in case of ARI. We defined a transmission if two persons had overlapping periods with symptoms and used additional definitions for sensitivity analyses. In total, 77 households participated with 282 persons. We observed 277 transmission events for ARI and 23 for AGE. In most cases, the first infected person in a household was a child (ARI: 63%, AGE: 53%), and the risk of within-household transmission was two times higher when the index case was a child. In 26 ARI-transmission events, pathogens were detected for both cases; hereof in 35% (95% confidence interval (17–56%)) the pathogens were different. Thus, symptomatic infections in household members, apparently linked in time, were in 2/3 associated with the same pathogens.
Morphophysiological seed dormancy inHeptacodium
Authors: Geneve Robert L. , Kester Sharon T.
Published: February 2018

Heptacodium miconiodesis an endangered, monotypic genus in the Caprifoliaceae endemic to China. Species within the Caprifoliaceae have been shown to have morphological or morphophysiological dormancy.Heptacodiumseeds had an underdeveloped embryo at the time of fruit dispersal with an embryo that occupied approximately 12% of the seed length. Cold (8 weeks at 5°C) and warm (8 weeks at 20°C) stratification was effective for dormancy release, but embryo growth prior to germination only occurred at warm temperatures (20°C). Gibberellic acid treatment partially substituted for cold stratification. Final seed germination percentage was not different after warm or cold stratification; however, seeds initially exposed to cold stratification germinated faster and more uniformly. Cold stratified seeds reached 50% final germination approximately 55 days sooner than warm stratified seeds. Prior to radicle emergence, embryos grew to fill approximately 60% of the seed through an endosperm channel that occupied the centre portion of the endosperm. Cells in the endosperm channel had thinner cell walls and fewer storage vesicles compared with other endosperm cells. Channel cells formed a dissolution zone ahead of embryo elongation assumed to be involved with enzymatic hydrolysis of storage reserves. Based on these results, it was concluded thatHeptacodiumdisplays the characteristics of seeds with non-deep simple morphophysiological dormancy.
Development and Evaluation of a Cognitive Behavioural Intervention for Chronic Post-Stroke Insomnia
Authors: Herron Katie , Farquharson Lorna , Wroe Abigail , Sterr Annette
Published: February 2018

Background:Cognitive behavioural therapy for insomnia (CBTI) has been successfully applied to those with chronic illness. However, despite the high prevalence of post-stroke insomnia, the applicability of CBTI for this population has not been substantially researched or routinely used in clinical practice.Aims:The present study developed a ‘CBTI+’ protocol for those with post-stroke insomnia and tested its efficacy. The protocol also incorporated additional management strategies that considered the consequences of stroke.Method:A single-case experimental design was used with five community-dwelling individuals with post-stroke insomnia. Daily sleep diaries were collected over 11 weeks, including a 2-week baseline, 7-week intervention and 2-week follow-up. The Insomnia Severity Index, Dysfunctional Attitudes and Beliefs About Sleep Scale, Epworth Sleepiness Scale, Fatigue Severity Scale and Stroke Impact Scale were administered pre- and post-treatment, as well as at 2-week follow-up.Results:At post-treatment, three participants no longer met diagnostic criteria for insomnia and all participants showed improvements on two or more sleep parameters, including sleep duration and sleep onset latency. Three participants showed a reduction in daytime sleepiness, increased quality of life and reduction in unhelpful beliefs about sleep.Conclusions:This study provides initial evidence that CBTI+ is a feasible and acceptable intervention for post-stroke insomnia. Furthermore, it indicates that sleep difficulties in community-dwelling stroke populations are at least partly maintained by unhelpful beliefs and behaviours. The development and delivery of the CBTI+ protocol has important clinical implications for managing post-stroke insomnia and highlights directions for future research.
A Poetic Record of the Rajput Rebellion, c. 1680
Authors: TALBOT CYNTHIA
Published: February 2018

Through comparison of three poetic texts describing the career of Rana Raj Singh of Mewar (r. 1652–1680), this paper demonstrates how representations of Aurangzeb could vary dramatically even when they were produced for the same Rajput court. Much of the paper focuses on Rāj-vilās, a vernacular-language work with a lengthy account of conflict between Aurangzeb and the Rajput lords of Marwar and Mewar. Rāj-vilās is also noteworthy for its negative portrayal of the Mughal emperor, whom it castigates as a wicked killer of kin who was duplicitous and vengeful. Sometimes thought to be modern constructions, the criticisms of Aurangzeb found in Rāj-vilās reveal that certain ideas about Indian historical figures have continued to be deployed and repurposed over the centuries. Yet Rajput views during Aurangzeb's lifetime were not uniformly unfavourable, as the Sanskrit texts Rāja-ratnākara and Rāja-praśasti attest. Although these two works resembled Rāj-vilās in covering the reign of Rana Raj Singh and were written at roughly the same time, they cast Aurangzeb in a considerably more positive light. This difference can be attributed to the fluctuating political relationship between the Mughal empire and the Mewar kingdom in the decade between 1677 and 1687, underscoring the need to carefully identify the historical contexts within which representations of Aurangzeb were produced and circulated.
AN APPROPRIATE APPROACH TO PRICING EUROPEAN-STYLE OPTIONS WITH THE ADOMIAN DECOMPOSITION METHOD
Authors: KE ZIWIE , GOARD JOANNA , ZHU SONG-PING
Published: February 2018

We study the numerical Adomian decomposition method for the pricing of European options under the well-known Black–Scholes model. However, because of the nondifferentiability of the pay-off function for such options, applying the Adomian decomposition method to the Black–Scholes model is not straightforward. Previous works on this assume that the pay-off function is differentiable or is approximated by a continuous estimation. Upon showing that these approximations lead to incorrect results, we provide a proper approach, in which the singular point is relocated to infinity through a coordinate transformation. Further, we show that our technique can be extended to pricing digital options and European options under the Vasicek interest rate model, in both of which the pay-off functions are singular. Numerical results show that our approach overcomes the difficulty of directly dealing with the singularity within the Adomian decomposition method and gives very accurate results.
Non-State Actors and the Implementation of the World Heritage Convention in Asia: Achievements, Problems, and Prospects
Authors: CHECHI Alessandro
Published: February 2018

The philosophy underlying the UNESCO World Heritage Convention of 1972 [WHC] consists in promoting a system of international co-operation in the context of which the States Parties commit to preserving the cultural treasures of “outstanding universal value” located within their territories. However, it is a fact that today many properties inscribed on the List set under the WHC are endangered. This paper will focus on the role played by “non-state actors” in the enforcement of the WHC. It will thus dwell upon the relationships between public and private interests, on the one hand, and between international and domestic legal orders, on the other. Its purpose is to map out and discuss the most salient problems about the involvement of non-state actors—particularly non-governmental organizations [NGOs] and private companies—in the monitoring and implementation of the WHC.
Den transgenerationalen Kreislauf der Gewalt durchbrechen
Authors: Vasileva Mira , Petermann Franz , Nitkowski Dennis , Petermann Ulrike
Published: February 2018

Zusammenfassung. Die langfristigen Folgen des transgenerationalen Kreislaufs der Gewalt belegen die Notwendigkeit für evidenzbasierte Interventionen, um aggressiv-dissoziales Verhalten von Jugendlichen mit Misshandlungs- und Gewalterfahrungen abzubauen. Es erfolgt ein Überblick über solche Therapieansätze und ihre Wirksamkeit. Es werden etablierte Programme dargestellt, deren Wirksamkeit in randomisierten Kontrollgruppenstudien überprüft wurde. Es existieren wenige manualisierte Therapierogramme, die den Abbau aggressiven Verhaltens bei Jugendlichen mit Misshandlungs- und Gewalterfahrungen evaluieren. Solche Therapierogramme bauen aggressives Verhalten bei Jugendlichen mit traumatischen Erfahrungen effektiv ab. Ihre Effektivität wurde in sehr unterschiedlichen Kontexten beurteilt, was einen Vergleich erschwert. Komponenten, die wiederholt erfolgreich angewandt wurden, waren die Aufklärung und Sensibilisierung gegenüber dem Thema „Gewalt“ und die Aufarbeitung einer Narration, die darauf abzielt, die traumatische Erfahrung in das autobiographische Gedächtnis einzuordnen und eine Habituierung zu fördern. Daraus wird geschlossen, dass eine Bearbeitung von Traumata häufig im Vorfeld notwendig ist, um aggressiv-dissoziales Verhalten zu verringern oder abzubauen. Für einen langfristigen Erfolg im Rahmen des Abbaus von aggressiv-dissozialem Verhalten sind jedoch Therapieverfahren notwendig, die sich spezifisch auf aggressiv-dissoziales Verhalten als primäres und nicht sekundäres Ziel (neben der Traumabewältigung) beziehen.
Aufbau der Ärgerkontrolle
Authors: Fern Julia , Petermann Franz
Published: February 2018

Zusammenfassung. Ärgerkontrolle bezeichnet die Fähigkeit, das Erleben und die Äußerung von Ärgergefühlen so zu gestalten, dass die Belastungen der sozialen Umwelt und der eigenen Person minimal ausgeprägt sind. Eine mangelhaft ausgeprägte Ärgerkontrolle kennzeichnet vor allem Kinder mit reaktiv-aggressivem Verhalten und beeinträchtigt die psychische Gesundheit und den Sozialkontakt eines Kindes stark. Obwohl aggressives Verhalten oft von Ärgergefühlen moderiert oder als die Folge von intensiven Ärgergefühlen betrachtet wird, liegt der Schwerpunkt von Therapieprogrammen für Sechs- bis 12-jährige mit aggressivem Verhalten auf dem Aufbau sozial kompetenten Verhaltens. Am Beispiel von drei deutschsprachigen verhaltenstherapeutischen Programmen wird der Frage nachgegangen, inwiefern und in welcher Form die Bewältigung von Ärger und Wutimpulsen in diesen Therapieprogrammen für Kinder mit aggressivem Verhalten berücksichtigt sind. Es zeigt sich, dass neben Techniken zur Steuerung von Ärgergefühlen insbesondere eine differenzierte Wahrnehmung des Ärgererlebens und die Vermittlung geeigneter Strategien zur Ärgerkontrolle wichtige Bestandteile der Therapieprogramme aggressiven Verhaltens im Kindesalter bilden.
Spin-orbit interaction of light induced by transverse spin angular momentum engineering
Authors: Shao Zengkai , Zhu Jiangbo , Chen Yujie , Zhang Yanfeng , Yu Siyuan
Published: February 2018

The investigations on optical angular momenta and their interactions have broadened our knowledge of light’s behavior at sub-wavelength scales. Recent studies further unveil the extraordinary characteristics of transverse spin angular momentum in confined light fields and orbital angular momentum in optical vortices. Here we demonstrate a direct interaction between these two intrinsic quantities of light. By engineering the transverse spin in the evanescent wave of a whispering-gallery-mode-based optical vortex emitter, a spin-orbit interaction is observed in generated vortex beams. Inversely, this unconventional spin-orbit interplay further gives rise to an enhanced spin-direction locking effect in which waveguide modes are unidirectionally excited, with the directionality jointly controlled by the spin and orbital angular momenta states of light. The identification of this previously unknown pathway between the polarization and spatial degrees of freedom of light enriches the spin-orbit interaction phenomena, and can enable various functionalities in applications such as communications and quantum information processing.
CryoEM structure of the human SLC4A4 sodium-coupled acid-base transporter NBCe1
Authors: Huynh Kevin W. , Jiang Jiansen , Abuladze Natalia , Tsirulnikov Kirill , Kao Liyo , Shao Xuesi , Newman Debra , Azimov Rustam , Pushkin Alexander , Zhou Z. Hong , Kurtz Ira
Published: February 2018

Na+-coupled acid–base transporters play essential roles in human biology. Their dysfunction has been linked to cancer, heart, and brain disease. High-resolution structures of mammalian Na+-coupled acid–base transporters are not available. The sodium-bicarbonate cotransporter NBCe1 functions in multiple organs and its mutations cause blindness, abnormal growth and blood chemistry, migraines, and impaired cognitive function. Here, we have determined the structure of the membrane domain dimer of human NBCe1 at 3.9 Å resolution by cryo electron microscopy. Our atomic model and functional mutagenesis revealed the ion accessibility pathway and the ion coordination site, the latter containing residues involved in human disease-causing mutations. We identified a small number of residues within the ion coordination site whose modification transformed NBCe1 into an anion exchanger. Our data suggest that symporters and exchangers utilize comparable transport machinery and that subtle differences in their substrate-binding regions have very significant effects on their transport mode.
Protecting tropical forests from the rapid expansion of rubber using carbon payments
Authors: Warren-Thomas Eleanor M. , Edwards David P. , Bebber Daniel P. , Chhang Phourin , Diment Alex N. , Evans Tom D. , Lambrick Frances H. , Maxwell James F. , Nut Menghor , O’Kelly Hannah J. , Theilade Ida , Dolman Paul M.
Published: February 2018

Expansion of Hevea brasiliensis rubber plantations is a resurgent driver of deforestation, carbon emissions, and biodiversity loss in Southeast Asia. Southeast Asian rubber extent is massive, equivalent to 67% of oil palm, with rapid further expansion predicted. Results-based carbon finance could dis-incentivise forest conversion to rubber, but efficacy will be limited unless payments match, or at least approach, the costs of avoided deforestation. These include opportunity costs (timber and rubber profits), plus carbon finance scheme setup (transaction) and implementation costs. Using comprehensive Cambodian forest data, exploring scenarios of selective logging and conversion, and assuming land-use choice is based on net present value, we find that carbon prices of $30–$51 per tCO2 are needed to break even against costs, higher than those currently paid on carbon markets or through carbon funds. To defend forests from rubber, either carbon prices must be increased, or other strategies are needed, such as corporate zero-deforestation pledges, and governmental regulation and enforcement of forest protection.
Genomic evidence of speciation reversal in ravens
Authors: Kearns Anna M. , Restani Marco , Szabo Ildiko , Schrøder-Nielsen Audun , Kim Jin Ah , Richardson Hayley M. , Marzluff John M. , Fleischer Robert C. , Johnsen Arild , Omland Kevin E.
Published: February 2018

Many species, including humans, have emerged via complex reticulate processes involving hybridisation. Under certain circumstances, hybridisation can cause distinct lineages to collapse into a single lineage with an admixed mosaic genome. Most known cases of such ‘speciation reversal’ or ‘lineage fusion’ involve recently diverged lineages and anthropogenic perturbation. Here, we show that in western North America, Common Ravens (Corvus corax) have admixed mosaic genomes formed by the fusion of non-sister lineages (‘California’ and ‘Holarctic’) that diverged ~1.5 million years ago. Phylogenomic analyses and concordant patterns of geographic structuring in mtDNA, genome-wide SNPs and nuclear introns demonstrate long-term admixture and random interbreeding between the non-sister lineages. In contrast, our genomic data support reproductive isolation between Common Ravens and Chihuahuan Ravens (C. cryptoleucus) despite extensive geographic overlap and a sister relationship between Chihuahuan Ravens and the California lineage. These data suggest that the Common Raven genome was formed by secondary lineage fusion and most likely represents a case of ancient speciation reversal that occurred without anthropogenic causes.
Resource acquisition and reproductive strategies of tropical forest in response to the El Niño–Southern Oscillation
Authors: Detto Matteo , Wright S. Joseph , Calderón Osvaldo , Muller-Landau Helene C.
Published: February 2018

The El Niño–Southern Oscillation (ENSO) is the largest source of interannual climate variability in much of the tropics. We hypothesize that tropical plants exhibit interannual variation in reproduction and resource acquisition strategies driven by ENSO that mirrors their seasonal responses. We analyze the relationship of leaf and seed fall to climate variation over 30 years in a seasonally dry tropical forest in Panama where El Niño brings warm, dry, and sunny conditions. Elevated leaf fall precedes the onset of El Niño, and elevated seed production follows, paralleling associations with dry seasons. Our results provide evidence of a shift in allocation from leafing to fruiting in response to a warming phase of ENSO. This shift may enable plants to take advantage of higher light availability, while coping with higher atmospheric water demand and lower water supply. These findings might be an indicator of adaptive strategies to optimize reproduction and resource acquisition.
A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
Authors: Chin Willy , Zhong Guansheng , Pu Qinqin , Yang Chuan , Lou Weiyang , De Sessions Paola Florez , Periaswamy Balamurugan , Lee Ashlynn , Liang Zhen Chang , Ding Xin , Gao Shujun , Chu Collins Wenhan , Bianco Simone , Bao Chang , Tong Yen Wah , Fan Weimin , Wu Min , Hedrick James L. , Yang Yi Yan
Published: February 2018

Polymyxins remain the last line treatment for multidrug-resistant (MDR) infections. As polymyxins resistance emerges, there is an urgent need to develop effective antimicrobial agents capable of mitigating MDR. Here, we report biodegradable guanidinium-functionalized polycarbonates with a distinctive mechanism that does not induce drug resistance. Unlike conventional antibiotics, repeated use of the polymers does not lead to drug resistance. Transcriptomic analysis of bacteria further supports development of resistance to antibiotics but not to the macromolecules after 30 treatments. Importantly, high in vivo treatment efficacy of the macromolecules is achieved in MDR A. baumannii-, E. coli-, K. pneumoniae-, methicillin-resistant S. aureus-, cecal ligation and puncture-induced polymicrobial peritonitis, and P. aeruginosa lung infection mouse models while remaining non-toxic (e.g., therapeutic index—ED50/LD50: 1473 for A. baumannii infection). These biodegradable synthetic macromolecules have been demonstrated to have broad spectrum in vivo antimicrobial activity, and have excellent potential as systemic antimicrobials against MDR infections.
Spin-controlled atom–ion chemistry
Authors: Sikorsky Tomas , Meir Ziv , Ben-shlomi Ruti , Akerman Nitzan , Ozeri Roee
Published: February 2018

Quantum control of chemical reactions is an important goal in chemistry and physics. Ultracold chemical reactions are often controlled by preparing the reactants in specific quantum states. Here we demonstrate spin-controlled atom–ion inelastic (spin-exchange) processes and chemical (charge-exchange) reactions in an ultracold Rb-Sr+ mixture. The ion’s spin state is controlled by the atomic hyperfine spin state via spin-exchange collisions, which polarize the ion’s spin parallel to the atomic spin. We achieve ~ 90% spin polarization due to the absence of strong spin-relaxation channel. Charge-exchange collisions involving electron transfer are only allowed for (RbSr)+ colliding in the singlet manifold. Initializing the atoms in various spin states affects the overlap of the collision wave function with the singlet molecular manifold and therefore also the reaction rate. Our observations agree with theoretical predictions.
Transcriptome analysis provides insights into xylogenesis formation in Moso bamboo (Phyllostachys edulis) shoot
Authors: Zhang Hui , Ying Ye-qing , Wang Jie , Zhao Xian-hai , Zeng Wei , Beahan Cherie , He Jun-bo , Chen Xiao-yang , Bacic Antony , Song Li-li , Wu Ai-min
Published: February 2018

Maturation-related changes in cell wall composition and the molecular mechanisms underlying cell wall changes were investigated from the apical, middle and basal segments in moso bamboo shoot (MBS). With maturation extent from apical to basal regions in MBS, lignin and cellulose content increased, whereas heteroxylan exhibited a decreasing trend. Activities of phenylalanine amonnialyase (PAL), cinnamyl alcohol dehydrogenase (CAD) and cinnamate-4-hydroxylase (C4H), which are involved in lignin biosynthesis, increased rapidly from the apex to the base sections. The comparative transcriptomic analysis was carried out to identify some key genes involved in secondary cell walls (SCW) formation underlying the cell wall compositions changes including 63, 8, 18, and 31 functional unigenes encoding biosynthesis of lignin, cellulose, xylan and NAC-MYB-based transcription factors, respectively. Genes related to secondary cell wall formation and lignin biosynthesis had higher expression levels in the middle and basal segments compared to those in the apical segments. Furthermore, the expression profile of PePAL gene showed positive relationships with cellulose-related gene PeCESA4, xylan-related genes PeIRX9 and PeIRX10. Our results indicated that lignification occurred in the more mature middle and basal segments in MBS at harvest while lignification of MBS were correlated with higher expression levels of PeCESA4, PeIRX9 and PeIRX10 genes.
In vivo virulence of Mycobacterium tuberculosis depends on a single homologue of the LytR-CpsA-Psr proteins
Authors: Malm S. , Maaß S. , Schaible U. E. , Ehlers S. , Niemann S.
Published: February 2018

LytR-cpsA-Psr (LCP) domain containing proteins fulfil important functions in bacterial cell wall synthesis. In Mycobacterium tuberculosis complex (Mtbc) strains, the causative agents of tuberculosis (TB), the genes Rv3484 and Rv3267 encode for LCP proteins which are putatively involved in arabinogalactan transfer to peptidoglycan. To evaluate the significance of Rv3484 for Mtbc virulence, we generated a deletion mutant in the Mtbc strain H37Rv and studied its survival in mice upon aerosol infection. The deletion mutant failed to establish infection demonstrating that Rv3484 is essential for growth in mice. Following an initial phase of marginal replication in the lungs until day 21, the Rv3484 deletion mutant was almost eliminated by day 180 post-infectionem. Interestingly, the mutant also showed higher levels of resistance to meropenem/clavulanate and lysozyme, both targeting peptidoglycan structure. We conclude that Rv3484 is essential for Mtbc virulence in vivo where its loss of function cannot be compensated by Rv3267.
Deletion of the Syncytin A receptor Ly6e impairs syncytiotrophoblast fusion and placental morphogenesis causing embryonic lethality in mice
Authors: Langford Michael B. , Outhwaite Jennifer E. , Hughes Martha , Natale David R. C. , Simmons David G.
Published: February 2018

Fetal growth and survival is dependent on the elaboration and propinquity of the fetal and maternal circulations within the placenta. Central to this is the formation of the interhaemal membrane, a multi-cellular lamina facilitating exchange of oxygen, nutrients and metabolic waste products between the mother and fetus. In rodents, this cellular barrier contains two transporting layers of syncytiotrophoblast, which are multinucleated cells that form by cell-cell fusion. Previously, we reported the expression of the GPI-linked cell surface protein LY6E by the syncytial layer closest to the maternal sinusoids of the mouse placenta (syncytiotrophoblast layer I). LY6E has since been shown to be a putative receptor for the fusogenic protein responsible for fusion of syncytiotrophoblast layer I, Syncytin A. In this report, we demonstrate that LY6E is essential for the normal fusion of syncytiotrophoblast layer I, and for the proper morphogenesis of both fetal and maternal vasculatures within the placenta. Furthermore, specific inactivation ofLy6ein the epiblast, but not in placenta, is compatible with embryonic development, indicating the embryonic lethality reported forLy6e−/−embryos is most likely placental in origin.
High Sensitivity of T-Ray for Thrombus Sensing
Authors: Sun Chi-Kuang , Chen Hui-Yuan , Tseng Tzu-Fang , You Borwen , Wei Ming-Liang , Lu Ja-Yu , Chang Ya-Lei , Tseng Wan-Ling , Wang Tzung-Dau
Published: February 2018

Atherosclerotic plaque rupture or erosion and subsequent development of platelet-containing thrombus formation is the fundamental cause of cardiovascular disease, which is the most common cause of death and disability worldwide. Here we show the high sensitivity of 200–270 GHz T-ray to distinguish thrombus formation at its early stage from uncoagulated blood. A clinical observational study was conducted to longitudinally monitor the T-ray absorption constant of ex-vivo human blood during the thrombus formation from 29 subjects. Compared with the control group (28 subjects) with uncoagulated blood samples, our analysis indicates the high sensitivity of 200–270 GHz T-Ray to detect thrombus with a low p-value < 10−5. Further analysis supports the significant role of platelet-activated thrombotic cascade, which modified the solvation dynamics of blood and occurred during the early coagulation stage, on the measured T-Ray absorption change. The ability to sense the thrombus formation at its early stage would hold promise for timely identification of patients at risk of various atherothrombotic disorders and save billions of lives.
Green synthesis of gold nanoparticles by thermophilic filamentous fungi
Authors: Molnár Zsófia , Bódai Viktória , Szakacs George , Erdélyi Balázs , Fogarassy Zsolt , Sáfrán György , Varga Tamás , Kónya Zoltán , Tóth-Szeles Eszter , Szűcs Rózsa , Lagzi István
Published: February 2018

Alternative methods, including green synthetic approaches for the preparation of various types of nanoparticles are important to maintain sustainable development. Extracellular or intracellular extracts of fungi are perfect candidates for the synthesis of metal nanoparticles due to the scalability and cost efficiency of fungal growth even on industrial scale. There are several methods and techniques that use fungi-originated fractions for synthesis of gold nanoparticles. However, there is less knowledge about the drawbacks and limitations of these techniques. Additionally, identification of components that play key roles in the synthesis is challenging. Here we show and compare the results of three different approaches for the synthesis of gold nanoparticles using either the extracellular fraction, the autolysate of the fungi or the intracellular fraction of 29 thermophilic fungi. We observed the formation of nanoparticles with different sizes (ranging between 6 nm and 40 nm) and size distributions (with standard deviations ranging between 30% and 70%) depending on the fungi strain and experimental conditions. We found by using ultracentrifugal filtration technique that the size of reducing agents is less than 3 kDa and the size of molecules that can efficiently stabilize nanoparticles is greater than 3 kDa.
Improved isolation strategies to increase the yield and purity of human urinary exosomes for biomarker discovery
Authors: Gheinani Ali Hashemi , Vögeli Mike , Baumgartner Ulrich , Vassella Erik , Draeger Annette , Burkhard Fiona C. , Monastyrskaya Katia
Published: February 2018

Circulating miRNAs are detected in extracellular space and body fluids such as urine. Circulating RNAs can be packaged in secreted urinary extracellular vesicles (uEVs) and thus protected from degradation. Urinary exosome preparations might contain specific miRNAs, relevant as biomarkers in renal and bladder diseases. Major difficulties in application of uEVs into the clinical environment are the high variability and low reproducibility of uEV isolation methods. Here we used five different methods to isolate uEVs and compared the size distribution, morphology, yield, presence of exosomal protein markers and RNA content of uEVs. We present an optimized ultracentrifugation and size exclusion chromatography approach for highly reproducible isolation for 50–150 nm uEVs, corresponding to the exosomes, from 50 ml urine. We profiled the miRNA content of uEVs and total urine from the same samples with the NanoString platform and validated the data using qPCR. Our results indicate that 18 miRNAs, robustly detected in uEVs were always present in the total urine. However, 15 miRNAs could be detected only in the total urine preparations and might represent naked circulating miRNA species. This is a novel unbiased and reproducible strategy for uEVs isolation, content normalization and miRNA cargo analysis, suitable for biomarker discovery studies.
Trichostatin A reverses the chemoresistance of lung cancer with high IGFBP2 expression through enhancing autophagy
Authors: Tang Dongfang , Yao Ruyong , Zhao Dandan , Zhou Lin , Wu Yun , Yang Yang , Sun Yifeng , Lu Liming , Gao Wen
Published: February 2018

Insulin-like growth factor (IGF) signaling plays an important role in tumorigenesis and metastasis. Here, we analyzed insulin-like growth factor (IGF) binding protein-2 (IGFBP2) expression in 81 lung cancer patients and 36 controls consisting of healthy and benign pulmonary lesion participants for comparison, then validated the IGFBP2 expression in additional 84 lung cancer patients, and evaluated the prognostic and chemoresistant significance of IGFBP2 in two cohorts respectively. Next we detected the reversal effect of trichostatin A (TSA) on chemoresistance in cell lines with high IGFBP2 expression. As a result, the mean expression of IGFBP2 in lung cancer patients was significantly higher than that in controls and increased with lung cancer progressed to advanced stage. In addition, high IGFBP2 expression was independently predictive for chemoresistance; over-expressed IGFBP2 enhances cell activity and TSA can reverse the chemoresistance induced by high IGFBP2 expression through enhancing autophagy. Furthermore, multivariate analysis showed that lung cancer patients whose blood IGFBP2 was higher had a poor survival outcome, with a hazard ratio of 8.22 (95%CI 1.78–37.92, P = 0.007) after adjustment for stage, histopathology, EGFR mutation, age, smoking and surgery.
Ferromagnetic domain walls as spin wave filters and the interplay between domain walls and spin waves
Authors: Chang Liang-Juan , Liu Yen-Fu , Kao Ming-Yi , Tsai Li-Zai , Liang Jun-Zhi , Lee Shang-Fan
Published: February 2018

Spin waves (SW) are low energy excitations of magnetization in magnetic materials. In the promising field of magnonics, fundamental SW modes, magnons, are accessible in magnetic nanostructure waveguides and carry information. The SW propagates in both metals and insulators via magnetization dynamics. Energy dissipation through damping can be low compared to the Joule heating in conventional circuits. We performed simulations in a quasi-one-dimensional ferromagnetic strip and found that the transmission of the propagating SW across the domain wall (DW) depends strongly on the tilt angle of the magnetization at low frequencies. When the SW amplitude is large, the magnetization tilt angle inside the DW changes due to the effective fields. The SW transmission, the DW motion, and the magnetization tilt angle couple to each other, which results in complex DW motion and SW transmission. Both SW filtering and DW motions are key ingredients in magnonics.
Genome-wide identification and expression analysis of GRAS family transcription factors in tea plant (Camellia sinensis)
Authors: Wang Yong-Xin , Liu Zhi-Wei , Wu Zhi-Jun , Li Hui , Wang Wen-Li , Cui Xin , Zhuang Jing
Published: February 2018

GRAS proteins are important transcription factors that play multifarious roles in regulating the growth and development as well as stress responses of plants. Tea plant is an economically important leaf -type beverage crop. Information concerning GRAS family transcription factors in tea plant is insufficient. In this study, 52 CsGRAS genes encoding GRAS proteins were identified from tea plant genome database. Phylogenetic analysis of the identified GRAS proteins from tea plant, Arabidopsis, and rice divided these proteins into at least 13 subgroups. Conserved motif analysis revealed that the gene structure and motif compositions of the proteins were considerably conserved among the same subgroup. Functional divergence analysis indicated that the shifted evolutionary rate might act as a major evolutionary force driving subfamily-specific functional diversification. Transcriptome analysis showed that the transcriptional levels of CsGRAS genes under non-stress conditions varied among different tea plant cultivars. qRT-PCR analysis revealed tissue and development stage-specific expression patterns of CsGRAS genes in tea plant. The expression patterns of CsGRAS genes in response to abiotic stresses and gibberellin treatment suggested the possible multiple functions of these genes. This study provides insights into the potential functions of GRAS genes.