bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Omics To Predict Changes During Cold Pressor Test
Authors: Kogelman Lisette J.A. , Ernst Madeleine , Falkenberg Katrine , Mazzoni Gianluca , Courraud Julie , Lundgren Li Peng , Laursen Susan Svane , Cohen Arieh , Olesen Jes , Hansen Thomas Folkmann
Published: March 2022

Background: The cold pressor test (CPT) is a widely used pain provocation test to investigate both pain tolerance and cardiovascular responses. We hypothesize, that performing multi-omic analyses during CPT gives the opportunity to home in on molecular mechanisms involved. Twenty-two females were phenotypically assessed before and after a CPT, and blood samples were taken. RNA-Sequencing, steroid profiling and untargeted metabolomics were performed. Each ‘omic level was analyzed separately at both single-feature and systems-level (principal component [PCA] and partial least squares [PLS] regression analysis) and all ‘omic levels were combined using an integrative multi-omics approach, all using the paired-sample design. Results: We showed that PCA was not able to discriminate time points, while PLS did significantly distinguish time points using metabolomics and/or transcriptomic data, but not using conventional physiological measures. Transcriptomic and metabolomic data revealed at feature-, systems- and integrative- level biologically relevant processes involved during CPT, e.g. lipid metabolism and stress response. Conclusion: Multi-omics strategies should be exploited in intervention studies, such as pain provocation tests, to gain knowledge on the biological mechanisms involved in complex traits.
ImageJ Assessment of Argon Laser Efficacy in Treatment of Mild Lower Eyelid Ectropion with Punctal Eversion
Authors: Salamah Moustafa , Enany Hesham , Hegab Mohammed Ahmed , bialy Hani AL
Published: March 2022

Purpose: To describe a new, simple and minimally invasive technique for correction of mild medial lower lid ectropion with punctal evrsion and measuring the efficacy of the technique by measuring the tear film meniscus pre and post argon laser treatment using imagej software.Methods: This study included 23 eyes, 19 patients with lower eyelid punctal eversion. All patients were treated by argon laser of lower medial conjunctiva. Tear film thickness was assessed using imagej software pre and post argon laser treatment.Results: There was a highly significant difference between before and after argon laser treatment as regarding the height of tear film which was found to be significantly lower after argon laser treatment compared to before it (81.1pixels versus 193.1pixels respectively) (p< 0.001). Conclusion: In conclusion, argon laser to lower medial palpebral conjunctiva is a simple, easy, safe, and effective procedure which can be applied as a sole treatment in early cases of punctal eversion with no or mild medial canthal tendon laxity.Clinical Trials Registration no clinical trial registration.
Microarray analysis of mRNA expression profiles in liver of ob/ob mice with real-time atmospheric PM2.5 exposure
Authors: Lin Lisen , tian li , Li tianyu , sun mengqi , duan junchao , Yu Yang , sun zhiwei
Published: March 2022

Epidemiological studies have demonstrated the association between exposure to fine particulate matter (PM2.5) and the onset of Non-alcoholic fatty liver disease (NAFLD). However, the potential biological mechanism is largely unknown. Our study was aimed to explore the impact of PM2.5 on the transcriptome level in the liver of ob/ob mice by atmosphere PM2.5 whole-body dynamic exposure system, and meanwhile preliminarily investigated the effects of metformin intervention in this process. 3,574 differentially expressed genes (DEGs) was screened out by microarray analysis (p<0.05, FC>1.5). KEGG pathway enrichment analysis showed that these DEGs were mainly enriched in cancers, infectious diseases and signal transduction, and the most significant pathway were thyroid hormone signaling pathway, chronic myeloid leukemia and metabolic pathways. Then, 12 hub genes were gained through weighted gene correlation network analysis (WGCNA) and verified by qRT-PCR. The expression of 5 genes in darkslateblue module (cd53, fcer1g, cd68, ctss, laptm5) increased after PM2.5 exposure, and decreased after metformin intervention. They were related to insulin resistance, glucose and lipid metabolism and other liver metabolism, and also neurodegenerative diseases. This study provided valuable clues and possible protective measures to the liver damage in ob/ob mice caused by PM2.5 exposure, and further research is needed to explore the related mechanism in detail.
Genetic dissection of behavioral traits related to successful training in drug-detection dogs
Authors: Matsumoto Yuki , Konno Akitsugu , Ishihara Genki , Inoue-Murayama Miho
Published: March 2022

Drug detection dogs play integral roles in society; however, the interplay between their behaviors and genetic characteristics remains uninvestigated. To profile the genetic traits associated with various behaviors related to the successful training of drug detection dogs, we collected and analyzed more than 230,000 genetic variants from 326 dogs belonging to the German Shepherd or Labrador Retriever breeds. Behavioral breed differences were observed in ‘friendliness to humans’ and ‘tolerance to dogs’. A strong positive correlation was observed in the genomic heritability of behavioral traits between breeds (Pearson’s r = 0.964, P = 0.008), indicating a similar degree of genetic influence on the behavioral traits between breeds. A genome-wide association study identified 18 single nucleotide polymorphisms potentially associated with drug detection abilities and three behavioral traits (interest in the dummy, tolerance to dogs, and friendliness to humans) related to drug detection abilities. Among them, 61 protein coding genes, including those associated with anxiety-related or exploration behavior in mice, such as Atat1 and Pfn2, were located surrounding the candidate polymorphisms. These findings highlight genetic characteristics associated with behavioral traits that are important for the successful training of drug detection dogs, which might support improved breeding and training of these dogs.
Highly Integrated Bionic Prostheses Resolve The Thermal Asymmetry Between Residual Amputated And Contralateral Limbs
Authors: Lang Victoria , Munoz-Novoa Maria , Ortiz-Catalan Max
Published: March 2022

Residual limbs after amputation present colder temperatures than unaffected contralateral limbs. This temperature asymmetry has been attributed to autonomic and cognitive factors, such as changes in body representation. An ideal limb replacement should restore the body representation and resolve the temperature asymmetry, but conventional prostheses, commonly characterized as disembodied, fail to do so. Neuromusculoskeletal prostheses are a new concept of artificial limbs that directly interface with the user’s nerves, muscles, and skeleton, and are operated in daily life by bidirectionally transferring control and somatosensory information. Here, we show that the temperature asymmetry is resolved in neuromusculoskeletal but not conventional prostheses. Increased ownership and agency inducing embodiment of the neuromusculoskeletal prostheses and could be a potential explanation of the phenomenon, suggesting a cognitive neural basis of the temperature asymmetry commonly found with amputation.
Financial inclusion and their role in renewable energy and non-renewable energy consumption in China: Exploring the transmission channels
Authors: Li Yukun , Chen Jian , Sohail Muhammad Tayyab
Published: March 2022

China is the most polluted economy in the world, facing the challenges of increased CO2 emissions. In this way, energy consumption is a key factor in CO2 emissions. To this end, this study empirically examines the effects of financial inclusion and education on energy consumption employing the ARDL approach for China during the period 1995–2019. The results suggest that financial inclusion has a positive effect on renewable energy consumption, but a negative impact on renewable energy and total energy consumption. The results show that education reduces non-renewable consumption and increases renewable energy consumption in the long-term. Long-run and short-run findings are also consistent and robust in the sensitivity analysis. Based on findings, China should invest in the education sector and increase financial inclusion to reduce energy consumption to meet environmental sustainability.
First observation of the proton emitter 116La: Evidence for strong neutron-proton pairing from proton separation energies and emission probabilities
Authors: Zhang Wei , Cederwall Bo , AKTAS Ozge , Liu Xiaoyu , Ertoprak Aysegül , Nyberg Ayse , Auranen Kalle , Alayed Betool , Badran Hussam , Boston Helen , Doncel Maria , Forsberg Ulrika , Grahn Tuomas , Greenlees Paul , GUO Song , Heery Jacob , Hilton Joshua , Jenkins David , Julin Rauno , Juutinen Sakari , Luoma Minna , Neuvonen Olavi , Ojala Joonas , Page Robert , Pakarinen Janne , Paul Edward , Petrache Costel , Rahkila Panu , Ruotsalainen Panu , Sandzelius Mikael , Sarén Jan , Szwec Stuart , Tann Holly , Uusitalo Juha , Wadsworth Robert
Published: March 2022

The discovery of the new proton emitter $^{116}_{~57}$La$_{59}$, 23 neutrons away from the only stable La isotope, $^{139}_{~57}$La$_{82}$, is reported. The $^{116}$La nuclei were synthesised in the fusion-evaporation reaction $^{58}$Ni($^{64}$Zn, p4n)$^{116}$La and identified via their proton radioactivity using the MARA mass spectrometer and the silicon detectors placed at its focal plane. Comparisons of the measured proton energy ($E_{p} = 718 \pm 9 $ keV) and half-life ($T_{1/2} = 50 \pm 22$ ms) with values calculated using %microscopic nuclear barrier penetration theory
the Universal Decay Law approach indicate that the proton is emitted with an orbital angular momentum $l=2$ and that its emission probability is enhanced relative to its closest, less exotic, odd-even lanthanum isotope ($^{117}_{~57}$La$_{60}$) while the proton-emission $Q$-value is lower. We propose this to be a signature for the presence of strong neutron-proton pair correlations in this exotic, neutron deficient system. The observations of $\gamma$ decays from isomeric states in $^{116}$La and $^{117}$La are also reported.
Accountability in healthcare in the Netherlands: A scoping review
Authors: Kerkvoorden Dennis Van , Ettema Roelof , Minkman Mirella
Published: March 2022

Accountability in health services is necessary for legitimacy, transparency, financial and managerial justification. To achieve the best possible outcomes for people at an affordable cost, healthcare services are continuously evolving. One development is the movement to person-centred integrated care to better serve people’s individual needs. This requires inter-organisational cooperation and service provision by domain-overarching networks and alliances. Accountability traditionally focuses on professionals and organisations. In the development towards inter-organisational networks, it is relevant to explore which accountability approaches are appropriate for local inter-organisational healthcare governance. Therefore, in this study we provide insight into the state of play of accountability in Dutch healthcare.MethodsFor systematically mapping and addressing the exploratory nature of the topic of accountability in healthcare in the Netherlands, we executed a scoping review. Databases were searched from January 2009 to March 2021. The following aspects of accountability are mapped: keywords of accountability, domains, stakeholders and target audiences, the aim (why), content (what) and method (how) of accountability, who is accountable to whom, and reported issues in accountability in Dutch healthcare practice. ResultsForty-one studies were included, of which twenty were in the hospital domain. Definitions of accountability were mostly absent. Involved stakeholders include mainly medical and managerial professionals in practice and supervisory boards. Most frequently reported issues were the relationship between performance management and accountability, different views of stakeholders about both accountability and perverse effects. Accountability covering integrated care was rarely described.ConclusionsAccountability is not only a method for legitimacy or transparency. It relates to how to judge performance, on what basis and towards whom. Current, accountability practice does not focus on or comply with the characteristics of integrated service delivery and is merely single profession or single organisation focussed. Issues like perverse effects, absence of trust, and preventing bureaucracy are relevant for further development of effective accountability. New guiding principles for accountability that suit integrated health-service delivery in the Netherlands should be developed.
Aldehyde Dehydrogenase 2 Activator Augments the Beneficial Effects of Empagliflozin in Diabetes Aassociated HFpEF
Authors: Pan Guodong , Roy Bipradas , Giri Shailendra , Lanfear David E , Thandavarayan Rajarajan A. , Guha Ashrith , Ortiz Pablo , Palaniyandi Suresh Selvaraj
Published: March 2022

Objectives: To ameliorate diabetes mellitus associated heart failure with preserved ejection fraction (HFpEF), we plan to lower diabetes-mediated oxidative stress-induced 4-hydroxy-2-nonenal (4HNE) accumulation by pharmacological agents that either decrease 4HNE generation or increase its detoxification.Background: 4HNE, a cellular reactive carbonyl species (RCS), was significantly increased in diabetic hearts due to a diabetes-induced decrease in 4HNE detoxification by aldehyde dehydrogenase (ALDH)2, a cardiac mitochondrial enzyme that metabolizes 4HNE. Therefore, hyperglycemia-induced 4HNE is critical for diabetes-mediated cardiotoxicity and we hypothesize that lowering 4HNE ameliorates diabetes associated HFpEF. Methods: We fed high-fat diet to ALDH2*2 mice which have intrinsically low ALDH2 activity to induce type-2 diabetes. After 4 months of diabetes, the mice exhibited features of HFpEF and along with increased 4HNE adducts and we treated them with vehicle, empagliflozin (EMP) (3mg/kg/d) to reduce 4HNE and Alda-1 (10mg/kg/d), an ALDH2 activator to enhance ALDH2 activity as well as a combination of EMP + Alda-1 (E + A), via subcutaneous osmotic pumps. After 2 months of treatments, cardiac function was assessed by conscious echocardiography before and after exercise stress. Results: EMP + Alda-1 improved exercise tolerance, diastolic and systolic function, 4HNE detoxification and cardiac liver kinase B1 (LKB1)-AMP-activated protein kinase (AMPK) pathway in ALDH2*2 mice with diabetes associated HFpEF. This combination was even more effective than EMP alone. Conclusions: Our data indicate that ALDH2 activation along with the treatment of hypoglycemic agents may be a salient strategy to alleviate diabetes associated HFpEF.
Effects of Parenting Mode on Student Adaptability: The Mediating Effect of Irrational Beliefs
Authors: Kong Hua , Hongwang Xu , Yujian Deng , Xuefeng Wang , Wei Zhang
Published: March 2022

Backgrounds: When facing the new growth field of university, some college students will quickly form good university adaptation, while others will have maladaptation behavior. The students’ adaptations of to the growth environment in universities and smooth transition to adulthood are the important topics in recent years. Methods：Present study introduces irrational beliefs to investigate the effects of parenting mode on maladaptation of university students. The questionnaires based on simplified parenting mode (Chinese), irrational belief and adaptability were administered in a survey of 510 university students in Zhanjiang on October, 2021, by using the random sampling method. Parenting mode was used as the independent variable, while the emotionally warm, overprotective and rejecting types were used as the indices. Further, the irrational beliefs including summary comments, awful beliefs and low tolerance to setbacks as well as maladaptation were included in the mediation model for analysis.Results: The results showed that emotionally warm parenting mode was negatively correlated with summary comments (r = -0.334), awful beliefs (r = -0.108) and low tolerance to setbacks (r = -0.153). The rejection parenting mode was negatively related with absolute requirements (r = -0.143), and learning motivation (r = -0.157), interpersonal adaptation (r = -0.283) and physical and psychological adjustment(r=-0.083). Overprotection was negatively correlated with absolute requirements (r = -0.042) and interpersonal adaptation (r = -0.042). The mediating effect of irrational beliefs (low tolerance to setbacks, awful beliefs and absolute requirements), the lower and upper limits of Bootstrap confidence interval were 0.141 and 0.532, respectively, excluding 0, which indicated that the mediating effect is true. Conclusion: Through the analysis of the data, this study believes that irrational beliefs such as low tolerance to setbacks, awful beliefs and absolute requirements mediate the effects on school adjustment. Parenting mode can influence adaptability of university students. Emotionally warm parents strengthen the adaptability of university students. By contrast, negative parenting modes such as overprotection and rejection inculcate irrational beliefs, resulting in maladaptation of university students.
Application of Machine Learning for Inter Turn Fault Detection in Pumping System
Authors: Dutta Nabanita , Kaliannan Palanisamy , Shanmugam Paramasivam
Published: March 2022

Pump fault diagnosis is essential for maintenance and safety of the device as it is an important appliance used in various major sectors. Fault diagnosis at proper time can reduce maintenance cost and save energy. In this article a Simulink model based on mathematical equations has been built for analyzing the effects of parameter estimation of three phase induction motor based centrifugal pump in inter turn fault condition. The inter turn fault causes huge increase of current which severely affects the parameters of both motor and pump and these have been analysed by simulation through Matlab Simulink model. Later, the results are verified by hardware in loop (HIL) based simulator. In this paper machine learning (ML) based artificial neural network (ANN) and ANFIS (ANN and Fuzzy) models have been applied for fault detection ANN and ANFIS based models provide a satisfactory level of accuracy. These models provide accurate training and testing results. Based on root mean square error (RMSE), R2, prediction accuracy and mean validation value these models are compared to find out which is more suitable for this experiment. Various supervised algorithms are compared with ANN, ANFIS and lastly found which is the most suitable for this experiment.
Cell surface accumulation of intracellular leucine proline-enriched proteoglycan 1 enhances odontogenic potential of human dental pulp stem cells
Authors: Kang Kyung-Jung , Min Tae-Jun , Choi Min-Jeong , You Tae Min , Lee Gyutae , Ko Seon-Yle , Jang Young-Joo
Published: March 2022

Primary dental pulp cells can be differentiated into odontoblasts, which are responsible for dentin formation and mineralization. Successful differentiation has been verified using a few markers. However, odontoblast-specific cell surface markers have not been fully studied yet. Previously, we have constructed monoclonal antibodies to identify cell surface molecules of odontoblasts by decoy immunization. Mass spectrometry confirmed that one of these antibodies, the anti-OD40 antibody, recognized LEucine PRoline-Enriched Proteoglycan 1 (LEPRE1) as a specific antigen. Among three alternative splicing isoforms of LEPRE1, this new antibody showed the most stable antigenic affinity for LEPRE1a, a canonical transcript. Anti-OD40 antibody could strongly bind to the cell surface as odontogenic differentiation progressed. Intracellular and cell surface accumulation of this protein completely disappeared during dentin maturation and mineralization. Overexpression and knock-down experiments showed that intracellular LEPRE1/OD40 should not accumulate too much and that it should move from ER to the cell surface for efficient odontogenic differentiation. Indeed, when LEPRE1/OD40 already located on the cell surface was blocked with antibody, odontogenic differentiation of human dental pulp cells was inhibited. In addition to its role in differentiation, our new antibody which only recognizes LEPRE1a isoform specifically will be useful in studying differentiated functions between LEPRE1a and other isoforms found in osteogenesis imperfecta and dentinogenesis imperfecta.
Perceptions and attitudes about antibiotic resistance in the general public and general practitioners in France
Authors: MENARD Colette , FEGUEUX Sophie , HERITAGE Zoë , NION-HUANG Michèle , BERGER-CARBONNE Anne , BONMARIN Isabelle
Published: March 2022

Background
During the last 20 years, France has taken important steps to tackle antibiotic resistance. These include national awareness campaigns for the general public, and supporting changes in terms of antibiotic prescription for healthcare practitioners. We conducted two surveys prior to the commencement of the upcoming 2022/2023 campaign, to explore the general public’s knowledge, attitudes and behaviours regarding antibiotics and also the perceptions and practices of general practitioners (GPs).
Methods
In 2019, we conducted a telephone survey in a national representative quota sample of 1204 persons aged over 15 years old living in metropolitan France, including an over-sample of 332 parents of children six years old or younger. In 2020, an online survey was conducted in a national representative sample of 388 GPs. Comparisons were made with similar telephone and online surveys conducted in 2010.
Results
Twenty-seven percent of respondents reported taking antibiotics during the previous year (31% in 2010). Sixty-five percent of GPs declared prescribing fewer antibiotics during the previous five years. However, 92% of GPs reported pressure from their patients for an antibiotic prescription (88% in 2010). The pressure from elderly patients increased notably between the two study periods. Twenty-three percent of respondents reported putting pressure on their GP (15% in 2010). All respondents expressed total trust in their GP irrespective of whether s/he had prescribed them antibiotics. Half knew that antibiotics act only on bacteria, and 38% understood precisely what antibiotic resistance is.
Conclusion
Although antibiotic use is decreasing in France, patient pressure on GPs to prescribe antibiotics has increased. GPs are key ambassadors in reducing antibiotic use. Awareness campaigns must target elderly patients in particular.
Pentatrichomonas hominis and other intestinal parasites in school-aged children: Clinical and coproscopic survey
Authors: Abdo Sarah Mohamed , Ghallab Marwa , Elhawary Nagwa , Elhadad Heba
Published: March 2022

Pentatrichomonas hominis (P.hominis) is a large intestinal flagellated protozoan infecting humans. Little is known about the epidemiology of P.hominis in Egypt, its association with gastrointestinal symptoms and the co-infection with other parasites. Demographic and clinical data were collected from 180 school-aged children. Parasitological examination of fecal samples was done using direct wet mount, formalin ethyl-acetate, Kato-Katz and cultivation on Jones' medium to detect P.hominis and associated parasitic infection. The diagnosis of P.hominis was confirmed using Giemsa stain and scanning electron microscopy. The prevalence of P. hominis was 13.8% (25 out of 180 children). The prevalence of parasitic co-infection was significantly higher in P.hominis infected (84%, 21 participants) than in non-infected children (56%, 87 participants).The presence of abdominal pain and diarrhea in P.hominis infected children was higher than in non-infected children (84% and 32% vs. 76% and 18%), respectively. The difference was not statistically significant. This is the first report of P.hominis in Egypt. The significant association between P.hominis and other intestinal parasites may highlight the pathogenicity of P.hominis. Further studies are needed to understand the epidemiology and pathogenicity of P.hominis.
A Green Approach to the Removal of Malachite Green Dye from Aqueous Medium Using Chitosan/Cellulose blend
Authors: Shetty Bhavyashri , R Yashodha S , Johns Jobish
Published: March 2022

The usage of cost effective, ecofriendly and highly efficient adsorbent to the removal of dye from aqueous solution is presented in this paper. This work focusses on the prospective use of Chitosan/Cellulose blend to remove the Malachite green (MG) from aqueous medium. Optimization of experimental conditions to find the maximum dye adsorption is studied in detail. Results revealed that the maximum adsorption of dye occurs within 30 minutes of the experiment. The SEM images exhibited a change in its surface morphology upon the adsorption of dye. The adsorption isotherm of MG clearly fits the Langmuir and Freundlich equations and follows pseudo-second-order rate equation. Freundlich isotherm exhibited the maximum adsorption capacity of 115.1 mg/g when compared to Langmuir isotherm. 98.65% of dye degradation is observed at room temperature for an adsorbent dosage of 0.2g per 30 ml initial dye concentration. Current analysis and its comparison studies with other reports on adsorbents concludes that, Chitosan/Cellulose blend can be considered as a cost effective choice for the removal of MG.
Study On The Causes Of Coal Mine Production Safety Risks
Authors: Zhang Ru , Jia Baoshan , Shen Zhuo , Su Guorui
Published: March 2022

This study analyzed the influence of risk factors of coal mine safety based on WSR (Wili-Shili-Renli) theory. We established a coal mine safety risk index system of the “human-machine-ring-tube” by applying the decision-making experimental analysis method to calculate the centrality of coal mine safety risk factors. Interpretative structural modeling was applied to determine the relationships among the coal mine safety risk factors. The improved CRITIC empowerment method was then applied to establish a coal mine safety risk cause model, and the degree of comprehensive impact was determined. The results showed that the main risk factors affecting coal mine safety were: three violations rates of personnel, safety inspection rate, safety management system, hidden danger rectification, equipment reliability rate, safety monitoring system operation status, comprehensive quality of personnel, personnel operation and safety awareness, and video monitoring system operation status.
Origin of thyrotropin-releasing hormone neurons that innervate the tuberomamillary nuclei
Authors: Sánchez-Jaramillo Edith , Wittmann Gábor , Menyhért Judit , Singru Praful , González Gabriela Berenice Gómez , Islas Eduardo Sánchez , Recendis Nashiely Yáñez , Cabrera Jaime Arturo Pimentel , Olea Martha León , Gereben Balazs , Fekete Csaba , Charli Jean Louis , Lechan Ronald Michael
Published: March 2022

Hypophysiotropic TRH neurons function as metabolic sensors that regulate the thyroid axis and energy homeostasis. Less is known about the role of other hypothalamic TRH neurons. As central administration of TRH decreases food intake and increases histamine in the tuberomammillary nuclei (TMN), and TMN histamine neurons are densely innervated by TRH fibers from an unknown origin, we mapped the location of TRH neurons that project to the TMN. The retrograde tracer, cholera toxin B subunit (CTB), was injected into the TMN E1-E2, E4-E5 subdivisions of adult Sprague Dawley male rats. TMN projecting neurons were observed in the septum, preoptic area, BNST, perifornical area, anterior PVN, peduncular and tuberal lateral hypothalamus (TuLH), suprachiasmatic nucleus and medial amygdala. However, CTB/pro-TRH178-199 double-labeled cells were only found in the TuLH. The specificity of the retrograde tract-tracing result was confirmed by administering the anterograde tracer, Phaseolus vulgaris leucoagglutinin (PHAL) into the TuLH. Double labeled PHAL-pro-TRH boutons were identified in all subdivisions of the TMN. TMN neurons double labeled for histidine decarboxylase ( Hdc )/PHAL, Hdc / Trhr , and Hdc / Trh . Further confirmation of a TuLH TRH neuronal projection to the TMN was established in a transgenic mouse that expresses Cre-recombinase in TRH-producing cells following microinjection of a Cre-recombinase–dependent AAV that expresses mCherry into the TuLH. We conclude that in rodents, the TRH innervation of TMN originates in part from TRH neurons in the TuLH and that this TRH population may contribute to regulate energy homeostasis through histamine Trhr- positive neurons of the TMN.
Plastic Impurities In Biowaste Treatment: Environmental And Economic Life Cycle Assessment Of A Composting Plant
Authors: Bottausci Sara , Magrini Chiara , Tuci Giulia Adele , Bonoli Alessandra
Published: March 2022

The study focuses on an Italian composting plant and aims to investigate the impact of the presence of plastic impurities in the collected biowaste on the environmental and economic performance of the plant. The study is divided into two main steps: firstly, a material flow analysis was conducted to quantify the number of impurities (e.g., conventional plastics and biodegradable plastics) before and after the composting process. Secondly, a Life Cycle Assessment (LCA) and a complementary Life Cycle Costing (LCC) of the composting process were conducted. The results of the material flow analysis confirmed the initial assumption that conventional plastic remains almost constant before and after the composting treatment, while biodegradable plastic almost disappears. As far as the life cycle analyses are concerned, the most environmentally damaging phases of the process were the shredding and mixing phases, while the operating costs (OPEX) contributed the most to the total annual costs of the company. Finally, a further scenario analysis was performed, assuming that the plastic contaminants in the treated biowaste consisted exclusively of biodegradable plastics. The comparison with this ideal scenario can support decision-makers to understand the potential improvements achievable by addressing the presence of plastic impurities in the biowaste. The results show that the treatment of plastic impurities cause relevant environmental and economic impacts, being responsible for 46% of the total waste to treat at the end of the process, almost 7% of the total annual costs covered by the plant owners and about 30% of all negative externalities.
Ion imprinted sodium alginate hydrogel beads enhanced with carboxymethyl cellulose and β-cyclodextrin to improve adsorption for Cu2+
Authors: Fan Yajun , Shen Dianling , Yan Yu , Hu Xiaopeng , Guo Yaping , Zhong Yujun , Li Zhiyang , Xie Lianwu
Published: March 2022

Using Cu2+ as template ion, three sodium alginate hydrogel beads including SA, SAC, and SAB beads were prepared with sodium alginate blending with different precursors of carboxymethyl cellulose and β-cyclodextrin, respectively. The effect of pH, adsorption time, initial concentration of Cu2+, adsorbent dosage, and coexisting ions on the adsorption efficiency of three hydrogel beads was investigated. The results indicated that adding carboxymethyl cellulose and β-cyclodextrin into skeleton of beads increased the toughness of the beads and improved the adsorption capacity of Cu2+. The adsorption rate of Cu2+ on SA beads was conformed to the pseudo-first order kinetic equation, while the adsorption behavior of Cu2+ on SAC or SAB beads could be confirmed to the pseudo-second-order kinetic equation. Compared with the saturated adsorption capacity 510 mg/g of Cu2+ on SA, the saturated adsorption capacity of Cu2+ on SAB and SAC reached 817 mg/g and 822 mg/g, respectively, with their Cu2+ adsorption efficiency over 95% at 25°C. Thus, SAC and SAB beads could be used as adsorption material for detecting and removing Cu2+ from wastewater.
Severe Neuro-COVID is associated with peripheral immune signatures, autoimmunity and neurodegeneration: a prospective cross-sectional study
Authors: Etter Manina , Martins Tomás , Kulsvehagen Laila , Pössnecker Elisabeth , Duchemin Wandrille , Hogan Sabrina , Sanabria-Diaz Gretel , Müller Jannis , Chiappini Alessio , Rychen Jonathan , Eberhard Noëmi , Guzman Raphael , MarianI Luigi , Keller Emanuela , Melie-Garcia Lester , Jelcic Ilijas , Pargger Hans , Siegemund Martin , Kuhle Jens , Oechtering Johanna , Eich Caroline , Tzankov Alexandar , Matter Matthias , Yaldizli Özgür , Lieb Johanna , Psychogios Marios , Berkemeier Caroline , Leuzinger Karoline , Hirsch Hans , Granziera Cristina , Pröbstel Anne-Katrin , Hutter Gregor
Published: March 2022

Growing evidence suggests that coronavirus disease 2019 (COVID-19) is associated with acute and long-term neurological sequelae. However, the underlying pathophysiological mechanisms resulting in central nervous system (CNS) derogation remain unclear, posing both diagnostic and therapeutic challenges. Here, we performed a cross-sectional study (NCT04472013) and multidimensional characterization of cerebrospinal fluid (CSF) and plasma-targeted proteomics in different Neuro-COVID severity classes with corresponding clinical and imaging data. COVID-19 patients displayed a plasma cytokine storm but a non-inflammatory CSF profile. Severely affected patients displayed signs of blood-brain barrier (BBB) impairment, elevated microglia activation markers and a polyclonal B cell response targeting self- and non-self antigens. Also, COVID-19 patients had decreased regional brain volumes associated with specific CSF and plasma parameters. We provide a multiparametric framework of Neuro-COVID severity classifiers. Collectively, this data identified several potentially actionable targets that may be addressed to prevent COVID-19-related neurological sequelae.
Postoperative Outcomes Of Minimally Invasive Adrenalectomy - Do Body Mass Index And Tumor Size Matter? A Single Center Experience
Authors: Giron Felipe , Rey Carlos , Rodriguez Lina , Conde Danny , Vanegas Marco , Toledo Sara , Nassar Ricardo M
Published: March 2022

Background: Since Gagner performed the first laparoscopic adrenalectomy in 1992, laparoscopy has become the gold-standard procedure in the treatment of adrenal surgical diseases. Literature indicates that the rate of intra- and postoperative complications are not negligible. This study aims to describe the single-center experience of adrenalectomies; and explore the associations between body mass index (BMI) and tumor volume in main postoperative outcomes. Methods: Retrospective observational study with a prospective database in which we described patients who underwent adrenalectomy between January 2015 and December 2020. Operative time, intraoperative blood loss, conversion rate, complications, length of hospital stay, and comparison of the number of antihypertensive drugs used before and after surgery were analyzed. Analysis of BMI and tumor volume with postoperative outcomes such as anti-hypertensive change (AHC) in drug usage and pre-operative conditions were performed. Results: 45 adrenalectomies were performed, all of them were carried out laparoscopically. 4 were performed as a robot-assisted laparoscopy approach. 19 were women and 26 were men. Mean age was 54.9 ± 13.8 years. The mean tumor volume was 95.698 mm3 (Range 3.75 - 1010.87). Mean operative time was shorter in right tumors (2.64 ± 0.75 hours ) than in left tumors (3.33 ± 2.73 hours). Pearson correlation was performed to assess the relationship between BMI and AHC showing a direct relationship between increased BMI and anti-hypertensive drugs usage decrease r(45)= 0.92, p > 0.05 CI 95%. Operative time, Higher tumor volume showed a longer operative time, r(45)=0.6 (p=0.000 CI 95%). Conclusions: Minimally invasive adrenalectomy appears to have an increased impact in patients with higher BMI in terms of postoperative anti-hypertensive usage. Tumor volume seems to be associated with higher operative time and blood loss leading possibly to increased rates of morbidity. Nevertheless, due to our limited number of patients, more studies are needed to replicate and validate these results.
The Effect of Tohoku Earthquake on Japan’s Bilateral Trade Distribution: Case Study of African Markets
Authors: BIYIK Onur
Published: March 2022

This paper examines the Japanese International Bilateral Trade Flow (JIBTF) in relation to the economic crisis, such as the Tohoku earthquake, and focuses on changes in the Distribution Networks of JIBTF (DNoJ) concerning African countries and Japanese Major Trade Partners (JMTP). The Harmonized System 6-digit level (“total” and product level) panel data from 2001 to 2019 are analyzed by employing the (structural) gravity method. The results of the study find that the Tohoku earthquake affects the DNoJ among Japanese trade partners. Specifically, Japanese trade intention with African markets decreases relatively. In other words, exporting to African countries has a negative interaction effect after 2015. On the contrary, this interaction effect on JMTP comparatively increases; thus, Japan relocates its export to other markets such as JMTP. The Tohoku earthquake shaped the JIBTF to be more integrated with the Asia-Pacific and the EU regions, instead of African markets.
A highly sensitive and versatile fluorescent biosensor for pathogen nucleic acid detection based on toehold-mediated strand displacement initiated primer exchange reaction
Authors: Zhang Ye , Li Zihua , Su Wanting , Zhong Guangzhi , Zhang Xiaohe , Wu Yuan , Situ Bo , Xiao Yunju , Yan Xiaohui H , Zheng Lei
Published: March 2022

Existing methods for pathogen nucleic acid detection, such as PCR, require complicated operation and expensive facility. Here, we report a simple and versatile strategy for highly sensitive detection of pathogen nucleic acid based on toehold-mediated strand displacement initiated primer exchange amplification (t-PER). In the presence of target, the blocked hairpin substrate would be released by toehold-mediated strand displacement, which triggered the primer exchange reaction amplification. Then, multiple long tandem-repeat single-strands generated by PER could open the molecular beacon to recover the fluorescence signal. Based on the target selection and conditions optimization, the developed platform could detect down to 18 fM of target in 1 hour of process time with high discrimination factor. It is also successful applied for direct detection of HPV from clinical cervical swab samples, the results were consistent with those obtained from RT-PCR. Moreover, the versatility and clinical feasibility of this method was further confirmed by measuring Epstein-Barr virus, Hepatitis B virus, and Ureaplasma Urealyticum from different clinical samples (serum samples and urine samples). This simple platform enables specific and sensitive detection of pathogen nucleic acid in a format that might hold great potential for point-of-care infection diagnosis.
Rubus idaeus extract improves symptoms in knee osteoarthritis patients: results from a double blind randomized clinical trial
Authors: Henrotin Yves , Cozannet Romain Le , Fança-Berthon Pascale , Truillet Romain , Cohen-Solhal Martine , DunnGalvin Gillian , Grouin Jean-Marie , Doolan Andrea
Published: March 2022

BackgroundOsteoarthritis (OA) is the most frequent cause of disability in elderly people. In daily practice, the main objective of the physician is to reduce patient symptoms using treatments without adverse effects. However, the most prescribed treatment to manage OA symptoms remains nonsteroidal anti-inflammatory drugs which are associated with severe adverse effects. Therefore, we need a safe alternative to managing OA. One candidate is Rubus idaeus leaf extracts known to inhibit inflammatory responses.ObjectiveThis study aimed to investigate the effects of a 12-weeks intervention with an ethanolic extract from Rubus idaeus leaf on symptoms of knee osteoarthritis.MethodThe study was a randomized, double-blind, placebo-controlled, monocentric trial of 198 participants with femorotibial osteoarthritis. Participants were randomized equally to receive one daily during 3 months either 1 capsule of Rubus idaeus leaf extract 400 mg, 1 capsule of Rubus idaeus leaf extract 200 mg, or 1 capsule of placebo. The primary endpoint was an absolute change of the Western Ontario McMaster osteoarthritis index (WOMAC) pain subscale. The secondary endpoints were WOMAC global score, stiffness and function sub-scales, knee pain VAS score at walking, the Short Form (SF)-36, the Short Physical Performance Battery (SPPB), the 20-meter walk test, and the International Physical Activity Questionnaire (IPAQ) and Outcome Measures in Rheumatology Clinical Trials and Osteoarthritis Research Society International (OMERACT-OARSI) responders rate.ResultsIn the ITT population, WOMAC pain was not significantly modified by Rubus idaeus leaf extract compared to placebo. In contrast, Rubus idaeus leaf extract 400 mg after 12 weeks of treatment significantly reduced pain measured by the VAS. The mean pain decrease induced by Rubus ideaus leaf extract was over − 7 mm which is clinically relevant and reached clinically statistical difference compared to placebo with the highest dose.ConclusionRubus idaeus leaf extract was well tolerated and effective to relieve pain in a patient with knee osteoarthritis.Trial registration: NCT03703024 https://clinicaltrials.gov/ct2/show/NCT03703024 (11/10/2018).
Research through action: research protocol on the implementation of an integrated place-based primary intervention in a multicultural context.
Authors: Maillet Lara , Manceau Luiza Maria , Desjardins France , Gagnon Suzanne , Naraziah Lavanyah , Malham Sabina Abou
Published: March 2022

Our project specifically aims at improving the adaptation capacity of primary services to answer the complex needs of vulnerable people. The aim is to co-build, with our collaborators and partners, environment-based strategies enabling the coordination of primary services offered by health, social and community responders all the while ensuring the continuity of care (informational, relational, and contact) of vulnerable people, especially refugees. A particular attention is brought toward the experiential knowledge of the health system users. Therefore at least three citizen-partners are part of the research team since the beginning of the project.Goals: This research-action aims at implementing a “meshing innovation”, meaning an intervention that is integrated, lasting, and based on the needs of a community located in a Sherbrooke neighbourhood in order to improve the care of refugee people in a continuum of primary services. To reach this goal, we have 5 specific objectives. The overall goal of this project is to improve the services trajectory in the health and social services network for refugee people in Quebec. The implementation of a meshing innovation, through this pilot project, is a first step toward this overall goal. Method: This project falls within a participative research approach, based on the symmetry principle of the researchers and field stakeholders’ knowledge. To do so, we choose three distinct sources of data: (1) literature review, (2) focus group (N=9 twice), and (3) semi-structured interviews (N=10).Expected results: Concretely, an Implementation guide of establishment strategies for integrated interventions enabling the improvement of primary care for refugees will be written and will constitute the basis for an appropriation workshop for the members of the two joint research networks focused on primary practices (RRAPPL) (Université de Laval and Université de Sherbrooke) and, eventually, in other fields interested in adapting and implementing these strategies. In addition to this guide, we wish to facilitate the care of refugees by family practitioners of the territory; therefore, many of them are part of the project. Explicit ties will help raising awareness toward other family practitioners working in medical clinics of the territory while trying to facilitate a specific attribution to these practitioners through the Quebec Family Doctor Finder (GAMF). With this project, we strongly hope to promote the vulnerability factors of refugees to the Health and Social Services Department (MSSS) and to the Quebec General Practitioners Federation (FMOQ) to change the priority at the GAMF. Finally, a scientific publication will be written for an international journal.
Summary and Analysis of 13 RAS-Associated Autoimmune Leukoproliferative Disease Clinical Features
Authors: FU lingling , Ma Jie , Yao JiaFeng , Jiang Jin , Wei Yunyun , Yang Bixi , Wang Ruixin , Li Hongmin , Wu Runhui
Published: March 2022

Ras-associated autoimmune leukoproliferative disease (RALD) is a clinical syndrome, also known as autoimmune lymphoproliferative syndrome (ALPS) type IV, which is a primary immunodeficiency disease. However, there is still controversy about whether RALD is a chronic benign lymphoproliferative disease or a precancerous lesion. The clinical characteristics, laboratory examination results, treatment and prognosis of children with RALD treated in Beijing Children's Hospital were analyzed retrospectively. We reported 13 patients with RALD in our single center. The male-to-female ratio was 1.6:1, and the median age of onset was 6 months (range 2 months- 29 months). Eight patients (61.5%) had KRAS somatic mutations, and five patients (38.5%) had NRAS somatic mutations. The most common features were splenomegaly (12/13 cases), autoimmune cytopenia (9/13 cases), mononucleosis (10/13 cases), recurrent infection (7/13 cases), skin involvement (4/13 cases) and intestinal lesions (4/13 cases). Thirteen children were treated with immunotherapy, including glucocorticoids, intravenous immunoglobulin, cyclosporine, and sirolimus. The median follow-up time was 50 months (range 3 months-62 months). Four children died (2 cases of respiratory failure, 1 case of intestinal perforation and bleeding, and 1 case of sepsis with sudden respiratory and cardiac arrest). Nine children survived, seven children were treated with sirolimus, and no children developed malignant tumors of the hematopoietic system. In conclusion, RALD requires long-term follow-up to monitor malignant tumors, and sirolimus is effective for treatment.
Characterizing Disparities in Risky Alcohol Use, Tobacco Smoking, E-Cigarette Use and Physical Inactivity Among Cancer Survivors in the United States
Authors: Kim Jiyeong , Keegan Theresa H
Published: March 2022

Purpose.
Unhealthy lifestyle behaviors are associated with inferior health outcomes among cancer survivors, including increased mortality. It is crucial to identify vulnerable subgroups, yet investigations have been limited. Thus, this study aimed to examine sociodemographic and clinical characteristics associated with risky health behaviors among cancer survivors.
Methods.
We used national survey data (Health Information National Trends Survey, HINTS 2017–2020) for 2,579 cancer survivors. We calculated the prevalence of risky alcohol use, current tobacco smoking, e-cigarette use, and not meeting physical activity guidelines. We performed weighted logistic regression to obtain multivariable-adjusted odds ratios (OR) for the association between each unhealthy behavior with sociodemographic and clinical characteristics.
Results.
Overall, 25% showed risky alcohol use, 12% were current tobacco smokers, 3% were current e-cigarette users, and 68% did not meet physical activity guidelines. Cancer survivors who were males, non-Hispanic Whites or African Americans, without a college education, not married, with comorbidities or psychological distress were more likely to have unhealthy behaviors. Those with lung disease or depression were 2 times more likely to smoke tobacco or e-cigarettes and those with psychological distress were more 1.6 times more likely to be physically inactive. Moreover, risky drinkers (OR = 1.75, 95% CI = 1.22–2.52) and e-cigarette smokers (OR = 16.40, 95% CI 3.29–81.89) were more likely to be current tobacco smokers.
Conclusions
. We identified vulnerable subpopulations of cancer survivors with multiple unhealthy lifestyle behaviors.
Implications for Cancer Survivors
. Our findings inform clinicians and program and policy makers of the subgroups of cancer survivors to target for multiple health behavior interventions.