bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting Retinopathy of Prematurity Risk Using Plasma Levels of Insulin-like Growth Factor 1 (IGF1), Tumor Necrosis Factor-Alpha (TNF-Alpha), and Neonatal Parameters
Authors: Cioboata Daniela Mariana , Costescu Oana Cristina , Manea Aniko Maria , Doandes Florina Marinela , Zaharie Mihaela , Popa Zoran Laurentiu , Costescu Sergiu , Stoica Florina , Boia Marioara
Published: August 2024

Background/Objectives: Retinopathy of Prematurity (ROP) remains a leading cause of vision impairment in premature infants, especially those with Respiratory Distress Syndrome (RDS) necessitating respiratory support. This study aimed to identify correlations between plasma levels of Insulin-like Growth Factor 1 (IGF1) and Tumor Necrosis Factor-alpha (TNF-alpha), and the risk of developing ROP. Additionally, it explored the association of ROP severity grades with plasma levels of glucose, lactate dehydrogenase (LDH), creatin phosphokinase (CPK), and other biomarkers, aiming to uncover predictive markers for ROP risk and severity in this population. Methods: This prospective study included premature neonates admitted with RDS requiring respiratory support, conducted over 18 months at the Neonatal Intensive Care Unit of the Louis Turcanu Emergency Clinical Hospital for Children, Timisoara. Plasma levels of IGF1 and TNF-alpha were measured on days 1 and 14 post-birth, alongside the initial assessment of glucose, LDH, and CPK levels. Results: Significant correlations were observed between lower gestational age and elevated LDH levels on day 7–10 (rho = −0.341, p = 0.0123) and between TNF-alpha levels at 2 weeks and ROP severity (rho = 0.512, p = 0.0004). Elevated IGF1 levels were protective against ROP, with Beta coefficients of 0.37 (p = 0.0032) for the first collection and 0.32 (p = 0.0028) for the second, suggesting their potential as biomarkers for ROP risk assessment. Higher levels of TNF-alpha at 2 weeks were associated with an increased risk of ROP (Beta = −0.45, p = 0.0014), whereas higher IGF1 levels offered protective effects against ROP, with Beta coefficients of 0.37 (p = 0.0032) for the first collection and 0.32 (p = 0.0028) for the second. Elevated LDH levels on day 7–10 post-birth were linked to an increased risk of ROP (Beta = 0.29, p = 0.0214). Conclusions: These findings highlight the potential of IGF1 and TNF-alpha as predictive biomarkers for ROP, offering avenues for early intervention and improved management strategies in this high-risk group.
Investigation of Optical Properties of Complex Cr-Based Hard Coatings Deposited through Unbalanced Magnetron Sputtering Intended for Real Industrial Applications
Authors: Chitanov Vasiliy , Kolaklieva Lilyana , Kakanakov Roumen , Cholakova Tetiana , Pashinski Chavdar , Kolchev Stefan , Zlatareva Ekaterina , Atanasova Genoveva , Tsanev Alexander , Hingerl Kurt
Published: August 2024

A preliminary investigation of the optical properties of complex Cr-based hard coatings and their connection with the compound structure of the coatings is presented. Cr/CrN, Cr/CrN/CrAlN, Cr/CrN/CrTiAlN, and Cr/CrN/CrAlTiN coating structures were deposited through unbalanced magnetron sputtering. Their mechanical properties, especially nanohardness, coefficient of friction, and adhesion, were measured. The structure and composition of the coatings were characterised through SEM, FIB, XPS, and TEM. Their optical properties were investigated by UV-VIS ellipsometry. The optical parameters’ phase difference Δ, the amplitude ratio Ψ, the frequency-dependent real (ε1(ω)) and imaginary (ε2(ω)) part of the dielectric function, and the corresponding refractive and extinction indexes n(ω) and k(ω) were measured and modelled via regression analysis implemented using the WVASE© software version 3.686. Satisfactory coincidence was achieved between the measured optical data and the corresponding fitting models. The physical polycrystalline optical constants of the industrial samples were extracted from modelling data with roughness. The analysis of the data showed that ellipsometric characterisation can be used in order to determine the physical properties of Cr-based hard coatings with complex structure and composition, as well as for repeatability control of the deposition process. However, more detailed research is needed to predict the composition based on measured optical properties.
Extreme High-Speed DED of AISI M2 Steel for Coating Application and Additive Manufacturing
Authors: Ko Min-Uh , Cüppers Julius , Schopphoven Thomas , Häfner Constantin
Published: August 2024

This work focuses on the development of the 3D Extreme High-Speed DED process (EHLA3D), a variant of the laser-based Directed Energy Deposition (DED-LB), for the processing of the material HSS M2. Characteristics for the EHLA3D process are feed rates of >20 m/min, high cooling rates, and layer thicknesses in the range of 100 µm. This work covers the three subsequent stages: (1) a process parameter study on single-track deposition, (2) development of coating parameters, and (3) development of parameters for AM. In scope of stage 2, a coating parameter with a powder mass flow of ṁ = 1.9 kg/h was achieved. A variation in the deposition angles indicates that the coating process is feasible within a tilted deviation of up to 20°. In stage 3, a process parameter with a deposition rate of ṁ = 0.4 kg/h was developed. The hardness results of the as-built specimen with 67 HRC exceeds the hardness of conventionally manufactured and heat-treated M2 steel. The results of this work indicate that the EHLA3D process can be potentially utilized for the additive manufacturing with the material M2 as well as for the productive deposition of anti-wear coatings on free-form surfaces.
Research on the Corrosion Resistance of Reactive Powder Concrete with Straw Fibers under Chloride Environment
Authors: Wang An , Sun Weixiang , Lu Miao , Peng Xi , Wang Hui , Ji Yao
Published: August 2024

Reactive powder concrete (RPC) is widely used in large-scale bridges, and its durability in coastal areas has become a significant concern. Straw fibers have been evidenced to improve the mechanical properties of concrete, while research on their influence on the chloride corrosion resistance of RPC is deficient. Therefore, it is essential to establish the relationships between the quantities and parameters of straw fibers and the properties of the resulting concrete. In this study, the mass loss rates (MLRs), the relative dynamic modulus of elasticity (RDME), the electrical resistance (R), the AC impedance spectrum (ACIS), and the corrosion rates of steel-bar-reinforced RPC mixed with 0%–4% straw fibers by volume of RPC were investigated. A scanning electron microscope (SEM) and X-ray diffraction (XRD) were used to analyze the corrosion of steel bars. The reinforced RPC specimens were exposed to a 3% NaCl dry-wet alternations (D-As) and 3% NaCl freeze-thaw cycles (F-Cs) environment. The results show that, after adding 1%–4% straw fibers, the setting time and slump flow of fresh RPC were reduced by up to 16.92% and 12.89%. The MLRs were −0.44%–0.43% and −0.38%–0.42%, respectively, during the D-As and F-Cs. The relationship between the RDME and the fiber volume ratio was the quadratic function, and it was improved by 9.34%–13.94% and 3.01%–5.26% after 10 D-As and 100 F-Cs, respectively. Incorporating 4% straw fibers reduced the R values of the reinforced RPC specimens by up to 22.90% and decreased the corrosion rates after 10 D-As and 100 F-Cs by 26.08% and 82.29%, respectively. The impedance value was also increased. Moreover, a dense, ultra-fine iron layer and α-FeO(OH) were observed in the rust of rebars by SEM and XRD, as the corrosion resistance of rebars was enhanced. The results indicate that straw fibers improved the corrosion resistance of RPC, which can serve as a protective material to inhibit concrete cracking and thereby prevent rebar oxidation. This study provides theoretical support for the investigation of surface phenomena in reinforced RPC with straw fibers.
Unbleached Nanofibrillated Cellulose as Additive and Coating for Kraft Paper
Authors: Lengowski Elaine Cristina , Bonfatti Júnior Eraldo Antonio , Simon Leonardo Coelho , Izidio Vitória Maria Costa , Andrade Alan Sulato de , Nisgoski Silvana , Muniz Graciela Inês Bolzon de
Published: August 2024

Although paper packages are biodegradable, their applications in food packaging are limited due to high affinity for absorbing moisture and the high permeability of gases and liquids with surroundings. Therefore, exploring the use biodegradable coatings such as nanocellulose to improve barrier is a relevant strategy. This study assessed the efficacy of unbleached nanofibrillated cellulose (NFC) as an additive to paper and coatings. Using NFC derived from unbleached eucalyptus pulp, a 5% mass addition to the paper pulp and a 2 mm wet nanocellulose coating to kraft paper handsheets made from pine pulp were investigated. In addition, nanocellulose films of similar thicknesses were appraised. The physical, morphological, mechanical, and thermal characteristics of the resulting papers were assessed. Incorporating NFC notably enhanced the morphology area of the paper by padding its pores, thus increasing its density by up to 48% and improving its water barrier properties by up to 50%. The mechanical strength showed significant enhancements, particularly in bursting and tensile strength with increases of up to 134% and 50%, respectively. Anyhow, the films exhibited lower bursting indices and no improvement in the tearing index. Nonetheless, the thermal stability of the handsheets with NFC coating meets the minimum requirements for food packaging.
Tensile Behavior and Microstructure of the 6082 Alloy Sheet with High-Temperature Aging Treatments
Authors: Xia Erli , Ye Tuo , Wang Lijun , Qiu Sawei , Tian Yiran , Wu Changping , Luo Jing , Wu Yuanzhi
Published: August 2024

The present study investigates the tensile behavior and microstructure evolution of the 6082 aluminum alloy aged with high temperature. A universal testing machine was applied to explore the tensile behavior, while features of the fracture surface were characterized via scanning electron microscopy (SEM). The microstructural evolution was assessed through optical microscopy (OM) and transmission electron microscopy (TEM). The findings illustrate that the 6082 alloy sheet achieves peak strength following treatment at 180 °C for 8 h for the 0° orientation specimen, with the yield strength and tensile strength reaching 345 MPa and 373 MPa, respectively. An increase in aging temperature results in a decline in strength, accompanied by an improvement in elongation. After the treatment at 330 °C for 0.5 h, the corresponding yield strength falls below 150 MPa, with elongation exceeding 12%. The alloy sheet consistently exhibits ductile fracture characteristics with various aging treatments. The aging processes have no obvious influence on grain morphology. The fibrous grain structure is responsible for the anisotropic mechanical properties. The alloy aged at 180 °C for 8 h demonstrates the greatest precipitate density with the smallest precipitate size. As the aging temperature increases, the precipitate distribution becomes less uniform, and the precipitates grow coarser, leading to a decline in the precipitate density and corresponding strength of the alloy. Furthermore, it is noted that smaller precipitates are more effective in suppressing the mechanical anisotropy of the alloy.
Deposition of W Nanoparticles by Magnetron Sputtering Gas Aggregation Using Different Amounts of H2/Ar and Air Leaks
Authors: Acsente Tomy , Matei Elena , Marascu Valentina , Bonciu Anca , Satulu Veronica , Dinescu Gheorghe
Published: August 2024

This work presents the synthesis of tungsten nanoparticles (W NPs) using a cluster source based on magnetron sputtering combined with gas aggregation (MSGA), operated with up to 81% H2 in the hydrogen/argon mixture used as a working gas. The results show that, with up to 41% H2 in discharge, the synthesis rate increases by more than 60 times, rapidly decreasing for over 50% H2 in discharge. The W dust is still produced for H2-dominated discharges (81%), and its deposition rate is small but not negligible (0.02 mg/h). The obtained W NPs are isolated, with the diameter decreasing from 50 nm to 15 nm when the amount of H2 in discharge is smaller than 41%. Over this value, the particles tend to agglomerate, forming structures similar to film-like deposits. Also, the diameter of the dust spots deposited on substrates depends on the H2 content of the discharge. This allows the efficient coating of substrates up to 26 mm wide by translating them in front of the MSGA cluster source exit aperture. Additionally, for 41% H2 in discharge, the influence of synthetic air leaks (0%–8.2%) in discharge was investigated. The deposition rate decreases rapidly (ceasing for around 6% air in discharge), and the obtained nanoparticles tend to agglomerate on the substrate (at 3.3% air content, the dust deposit has the aspect of a near-continuous film). Chemical composition investigations show a pronounced tendency for oxidation, nitridation, and oxynitride formation in the presence of air leaks.
Navigating the Challenges of Commodity Traps and Platform Economies: An Assessment in the Context of the Northern Black Forest Region and Future Directions
Authors: Koelmel Bernhard , Fischer Leon , Juraschek Emilia , Peuker Levi , Stemmler Noah , Vielsack Anton , Bulander Rebecca , Hinderer Henning , Kilian-Yasin Katharina , Brugger Tanja , Kühn Ansgar , Brysch Tanja
Published: August 2024

The transition to battery electric vehicles (BEVs) poses significant challenges for automotive suppliers, particularly in the Northern Black Forest Region, Germany’s largest industrial area. This study examines the risk of falling into the commodity trap and the impact of platform economics on these suppliers. A VUCA (volatility, uncertainty, complexity, ambiguity) analysis is used to assess the consequences of the open platform approach promoted by the Mobility in Harmony (MIH) Consortium. The methodology is based on a comprehensive literature review on commodity traps and platform economies, as well as an analysis of the MIH Consortium’s strategies for standardizing BEV components. The results show that while the MIH Consortium’s modularization and standardization efforts can reduce costs and facilitate mass production, they also intensify competition and limit differentiation, threatening the profit margins of smaller suppliers. The study highlights the importance of strategic positioning and innovation to counter these risks. It concludes with recommendations on how automotive suppliers can adapt to the evolving environment and be agile in responding to new technological standards and market dynamics.
Numerical Investigation of a UHPC Connection Detail for Simple for Dead Load and Continuous for Live Load Steel Bridges in Seismic Areas
Authors: Khodayari Abbas , Sadeghnejad Amir , Azizinamini Atorod
Published: August 2024

The simple for dead load and continuous for live load (SDCL) steel bridge system offers an accelerated construction solution for steel bridges. The available details for the SDCL steel bridge system consist of a cast-in-place normal strength concrete (NSC) diaphragm. This paper presents a study on developing a continuity detail SDCL system in seismic areas using ultra-high-performance concrete (UHPC) as the diaphragm to simplify construction, improve durability, and increase tolerances. This paper is part of a large study on the non-seismic and seismic application of the SDCL steel bridge system using the UHPC diaphragm. The numerical investigation and validated modeling techniques developed in the study were used in this paper to focus on the detail required for the seismic areas. A series of numerical models were developed and subjected to types of loadings that would occur in the bridge under earthquake excitations. Based on the analysis results, a set of design recommendations was developed. The UHPC connection includes simple girder-end detail, the straight development of deck reinforcement in the diaphragm, and partial use of UHPC in the diaphragm. The developed detail can improve the on-site construction time, durability, and constructability of SDCL steel bridge systems.
Changes in the Frequency of Dental Clinic Visits, Expenses, and Treatment Type during the COVID-19 Pandemic in Japan
Authors: Morita Ichizo , Sakuma Shigemitsu , Kondo Kanae
Published: August 2024

Few studies have reported nationwide changes in dental visit patterns during the COVID-19 pandemic. This study aimed to clarify the impact of the COVID-19 pandemic on the type of dental treatment in Japan based on trends in the frequency of dental clinic visits per month and dental expenses per month or day. An interrupted time-series analysis was performed to examine changes in data from the national database before and after the first declaration of a state of emergency. Between March and April 2020, the monthly dental expenses per patient (MDEPP) increased by approximately JPY 950 (JPY) (p < 0.001) in patients aged ≤64 years and ≥65 years. Dental expenses per treatment (DEPT) increased by approximately JPY 300–400 (p < 0.001) in both age groups. After April 2020, the slope of the regression line for DEPT significantly increased in those aged ≤64 years (p < 0.001) and in those ≥65 years (p = 0.024). The number of treatments per month (NTPM) increased after the declaration of a state of emergency. However, the decreasing trend in the regression line for NTPM was stronger in those aged ≤64 years (p < 0.001). The MDEPP, DEPT, and NTPM showed an increasing trend during the first state of emergency compared to those observed in the previous year. The proportion of patients with relatively severe disease may have increased because patients with mild disease refrained from undergoing dental examinations. These results suggest changes in the type of dental treatment during the COVID-19 pandemic.
Evaluation of Psychosomatic, Respiratory, and Neurocognitive Health in COVID-19 Survivors 12 Months after ICU Discharge
Authors: Germann Nicolas , Amozova Daria , Göhl-Freyn Kristina , Fischer Tim , Frischknecht Manuel , Kleger Gian-Reto , Pietsch Urs , Filipovic Miodrag , Brutsche Martin H. , Frauenfelder Thomas , Kahlert Christian R. , Schmid Dagmar A. , Albrich Werner C.
Published: August 2024

Patients who survive critical COVID-19 frequently report post-acute sequelae of COVID-19 (PASC) such as psychosomatic and neurocognitive health problems. The goal of this study was to identify clinical risk factors and other predictors for such long-term consequences in severely ill COVID-19 patients. Adult COVID-19 intensive care unit (ICU) survivors from August 2020 to May 2021 were enrolled. A broad range of clinical, laboratory and chest computed tomography (CT) data was collected during their ICU stays. The association between ICU predictors and psychosomatic, respiratory, and neurocognitive assessments 12 months after ICU discharge was analyzed using univariate regression analysis. In 17 patients (mean age 58.9 ± 11.4 years), laboratory markers (CRP, lymphocytes, hemoglobin), ICU severity (SOFA, SAPS II, need for mechanical ventilation), complications (ARDS), and lung CT data (ground-glass opacity) were promising predictors of depressive and anxiety symptoms, fatigue, and sleep problems. Recovery of psychosomatic health such as fatigue, depression, and anxiety correlated with lower levels of inflammation and high hemoglobin levels. ARDS, mechanical ventilation, and worse SOFA and SAPS II scores were further risk factors for depressive and anxiety symptoms. Our study identified novel associations such as pulmonary ground-glass opacity being positively associated with depression, anxiety, fatigue, and insomnia levels.
Book Review: Atella, V.; Scandizzo, P.L. COVID-19 Disruption and the Global Health Challenges; Elsevier Inc.: London, UK, 2023; ISBN: 978-0-443-18576-2
Authors: Novelli Giuseppe
Published: August 2024

The COVID-19 pandemic constituted an important watershed for modern society. The experience of the pandemic influenced all economic and social activities, suggesting the need to review and rethink new ways of managing and controlling zoonotic infections. The book provides a complete and accurate analysis of the pandemic event, providing answers to many questions about its origin, spread, and management by government systems, and finally proposes some solutions to prevent and help us be better prepared for the recurrence of new pandemic crises. Above all, it provides methods to manage pandemics more effectively.
RNA-Seq Unveils Cross-Talk among Stress Response Mechanisms during Leaf Color Transformation in ALS Resistant Sorghums
Authors: Weerasooriya Dilooshi K. , Bandara Ananda Y. , Liu Sanzhen , Tesso Tesfaye T.
Published: August 2024

Acetolactate synthase (ALS) inhibitor herbicides are among widely marketed herbicide chemistries that act both against grass and broad-leaved weeds. Sorghum (Sorghum bicolor (L.) Moench) variants carrying resistance to ALS inhibitor herbicides were developed as a post-emergence weed control solution in sorghum. However, some ALS-resistant lines exhibit noticeable interveinal chlorosis at seedling stage, leading to reduced vigor. Although the plants eventually recover at an advanced growth stage, this may be a source of concern for growers and can undermine adoption of the technology. This study was initiated to identify mechanisms related to the manifestation of this phenotype. Two ALS-resistant genotypes, one displaying a yellow phenotype and the other a normal green phenotype, were cultivated, and tissue samples were collected at four time intervals, with the final sampling occurring after the genotypes had fully re-greened. RNA was extracted from the tissue samples and subjected to RNA-Seq analysis. Differential gene expression analysis was carried out using DESeq2, and a selected set of genes were confirmed via qRT-PCR. Gene Ontology enrichment and SorghumCyc pathway analysis uncovered notable regulatory changes in genes associated with chloroplasts, plant defense responses, and hormonal networks in the yellow genotypes. The pattern of gene expression strongly mimicked responses under abiotic stresses. In addition, the findings offer new insights into the potential for sorghum genotypes resistant to environmental stresses to also exhibit tolerance to a range of additional stresses.
Fast One-Step Microwave-Assisted Synthesis of Iron-Doped ZnS for Photocatalytic Applications
Authors: Bailón-Ruiz Sonia J. , Cedeño-Mattei Yarilyn , Núñez-Colón Angelie M. , Torres-Torres Kerianys
Published: August 2024

Semiconductor Zn-based nanomaterials have emerged as promising agents for the photocatalytic degradation of organic pollutants in wastewater treatment. However, achieving efficient synthesis protocols capable of rapidly producing small structures directly in aqueous environments remains challenging. Microwave-assisted synthesis presents a viable solution by enabling one-step particle generation swiftly and directly in water through increased pressure, thereby easily elevating the boiling point. This study investigates the microwave-assisted one-step synthesis of pure and iron-doped ZnS nanoparticles and assesses their efficacy in photodegrading Quinoline Yellow (QY) in aqueous suspensions. The results demonstrate a significant degradation of QY in the presence of 1% iron-doped ZnS nanoparticles, achieving approximately 66.3% degradation with 500 ppm of doped nanoparticles after 270 min. These findings highlight the considerable potential of 1% iron-doped ZnS nanoparticles as effective nanocatalysts.
Experimental Validation for Mechanically Tunable Defect Bands of a Reconfigurable Phononic Crystal with Permanent Magnets
Authors: Yang Jeonggyu , Jo Soo-Ho
Published: August 2024

Phononic crystals (PnCs) have garnered significant attention due to their unique ability to control elastic waves in unconventional ways. One area of research focuses on utilizing defects within PnCs. Defects create new pass bands within band gaps, leading to concentrated wave energy within the defects. However, defect-mode-enabled wave localization is effective only at specific frequencies, limiting its usefulness when the frequencies of incident waves vary. Existing methods to mechanically tune defect bands involve changing the geometries of unit cells or defects or attaching elastic foundations, which necessitates the detachment and reattachment of certain structures depending on the engineering situation. Considering these challenges, this study introduces a novel approach that utilizes the reconfigurable PnC design, incorporating permanent magnets and ferromagnetic materials. The case study involves a one-dimensional PnC consisting of a long metal beam with rectangular block-shaped permanent magnets periodically arranged and attached to the beam by magnetic forces. A defect is created by shifting a subset of these block-shaped permanent magnets in parallel. The extent of this parallel movement alters the vibrating characteristics of the defect, facilitating the mechanical control of the defect bands in the defective PnC. The effectiveness of this approach is experimentally validated.
Recommendations for the Treatment of Multiple Sclerosis in Family Planning, Pregnancy and Lactation in Switzerland: Immunotherapy
Authors: Graber Michael , Panchaud Alice , Legardeur Helene , Derfuss Tobias , Friedli Christoph , Gobbi Claudio , Zecca Chiara , Granziera Cristina , Jelcic Ilijas , Hammer Helly Noemi , Bigi Sandra , Diem Lara , Kamber Nicole , Kana Veronika , Kuhle Jens , Müller Stefanie , Salmen Anke , Hoepner Robert , Do Canto Philipp , Théaudin Marie , Surbek Daniel , Pot Caroline , Chan Andrew
Published: August 2024

A large number of disease-modifying immunotherapies are available for the treatment of people with multiple sclerosis. Many disease-modifying immunotherapies show scarce or no safety data in pregnancy and breastfeeding and are labeled as being contraindicated during these periods in the Swiss summary of product characteristics. Some disease-modifying immunotherapies also have restrictions for male patients. Hence, family planning should always be considered in treatment decisions. If clinically necessary, the continuation of immunotherapy during pregnancy can be considered for some substances. In these situations, the “Good Off-Label Use Practice”, careful consideration of the benefit–risk profile, and interprofessional cooperation between the treating neurologist, obstetrician–gynecologist, and pharmacist/pharmacologist, ideally with the involvement of experienced centers, is necessary. Here, we present an update on disease-modifying immunotherapies in multiple sclerosis with a focus on family planning, pregnancy, and breastfeeding and provide consensus recommendations of the Medico-Scientific Advisory Board of the Swiss Multiple Sclerosis Society, the Swiss Neurological Society, and the Swiss Society for Gynecology and Obstetrics (represented by the Academy of Fetomaternal Medicine). These unified national recommendations are necessary, as guidelines from other countries differ and because of separate approval/reimbursement situations in Switzerland.
Population Biology of the Non-Indigenous Rayed Pearl Oyster (Pinctada radiata) in the South Evoikos Gulf, Greece
Authors: Pafras Dimitris , Theocharis Alexandros , Kondylatos Gerasimos , Conides Alexis , Klaoudatos Dimitris
Published: August 2024

The Atlantic pearl oyster Pinctada radiata (Leach, 1814), the first documented Lessepsian bivalve species to enter the Mediterranean basin, is present in various coastal areas in Greece, and constitutes, almost exclusively, a domestic commercial bivalve resource. The present study aimed to contribute to the limited information available on P. radiata population structure and dynamics in Hellenic waters, especially following the recent enforcement of legislation for regulation of its fishery. A total of 703 individuals were collected using scuba diving from the South Evoikos Gulf. The male-to-female ratio (1:1.70) significantly departed from 1:1. A higher probability for female prevalence was exhibited for shell heights over 50.77 mm. Significant differences were exhibited in the shell height–total weight relationship between the sexes. The fourth-year class was the dominant cohort, comprising 50.09% of the population, out of the seven age classes identified. Asymptotic length was estimated at 109.1 mm and growth index at 3.35, respectively. Longevity was estimated at 15.7 years, with natural mortality (M) at 0.39 and total mortality (Z) at 0.76. The probability of capture (LC50) was estimated at 50.72 mm at 2.8 years. Biological reference points FMSY and EMSY were higher than the fishing mortality and current exploitation rate, respectively, indicating the potential for further population exploitation.
Physicochemical Characteristics of Commercially Available Greek Yoghurts
Authors: Pappa Eleni C. , Kondyli Efthymia , Pappas Athanasios C. , Kyriakaki Panagiota , Zoidis Evangelos , Papalamprou Lida , Karageorgou Agori , Simitzis Panagiotis , Goliomytis Michael , Tsiplakou Eleni , Georgiou Constantinos A.
Published: August 2024

In the present study, the physicochemical characteristics of 108 yoghurts purchased from the Greek market have been assessed. Generally, the range of the mean pH values of samples was 3.58–4.64, of fat 0–10.8%, of protein 3.29–10.05%, of total solids 10.75–25.18%, and of ash 0.5–1.17%. Samples were categorized as strained and non-strained (traditional with a crust or plain without a crust). The milk origin was classified as being from sheep, goats, cows, mixture of sheep–goat–cow milk, or cow–donkey milk combination. A significant effect of species origin was determined for yoghurt physicochemical attributes, whereas geographical origin, mainland or island, affected yoghurt acidity only. Discriminant analysis revealed MDA, pH, acidity, syneresis, fat, and protein content and color lightness and redness as the traits responsible for the discrimination of yoghurts into milk-type classes, whereas fat, protein, and ash content, pH, and syneresis attributes were responsible for the discrimination into fat content classes. Yoghurt samples were sufficiently clustered according to their fat content, whereas protein content and species origin discriminated yoghurts to a lesser extent. This first in-depth descriptive research on a wide range of samples of the renowned Greek yoghurt showed that several physicochemical characteristics can be used for sample discrimination.
Design of Marine Cyclodepsipeptide Analogues Targeting Candida albicans Efflux Pump CaCdr1p
Authors: Ribeiro Ricardo , Fortes Sara , Costa Lia , Palmeira Andreia , Pinto Eugénia , Sousa Emília , Fernandes Carla
Published: August 2024

Fungal infections are a significant threat to human health and the environment. The emergence of multidrug-resistant strains of fungi and the growing prevalence of azole resistance in invasive fungal infections exacerbate the problem, with efflux pumps being a major cause of antifungal resistance and a prime target for several counteractive strategies. In Candida albicans, the ATP-binding cassette superfamily transporter CaCdr1p is the predominant efflux pump involved in azole resistance. Marine organisms have unique phenotypic characteristics to survive in challenging environments, resulting in biologically active compounds. The cyclodepsipeptides unnarmicin A and C have shown promising results as inhibitors of rhodamine 6G efflux in cells expressing CaCdr1p. Herein, a series of unnarmicin analogues were designed and docked against a CaCdr1p efflux pump based on the cryogenic electron microscopy structure available to select the most promising compounds. Analogue 33 was predicted to be the best considering its high affinity for the efflux pump and pharmacokinetic profile. These results pave the way for further synthesis and in vitro biological studies of novel unnarmicins seeking a synergistic effect with fluconazole.
Potential Impact of Metabolic Syndrome Control on Cardiovascular Risk in Elderly Patients with Diabetes: A Cross-Sectional Study
Authors: Nascimento Tânia , Espírito-Santo Margarida , Gonçalves Adriana , Pinto Ezequiel , De Sousa-Coelho Ana Luísa , Estêvão Maria Dulce
Published: August 2024

Metabolic syndrome (MS), a complex pathology with features like abnormal body fat distribution, insulin resistance, and dyslipidaemia, contributes to higher cardiovascular (CV) risk. A cross-sectional study including 87 individuals assessed CV risk score in elderly patients with type 2 diabetes and MS in Algarve, Portugal. The 10-year CV risk score was estimated using the ADVANCE risk score calculator. The reductions in CV risk score were estimated by adjusting the data inputted on the online tool to achieve systolic blood pressure (SBP) <130 or <120 mmHg, and LDL cholesterol <70 mg/dL Beyond waist circumference, the mean number of clinical features of MS was 3.14 ± 0.84, without significant sex differences. The mean CV risk score was 22.5% (CI: 20.3–24.7). Sex-specific analysis showed higher risk score in males (24.2%, CI: 21.3–27.0) vs. females (19.7%, CI: 16.2–23.3; p = 0.028). Hypothetical risk score reductions show that lowering SBP to <130 mmHg could significantly lower the risk score by an average of 9.2% (CI: 7.7–10.7), whereas 34.5% of the participants would be out of the diagnostic criteria for MS. When comparing each potential intervention with current risk score, all interventions significantly reduce the 10-year CV risk score. The study highlights the potential of blood pressure control in reducing CV risk score and the importance of multifaceted risk score reduction strategies.
Fluoroscopic-Guided vs. Multislice Computed Tomography (CT) Biopsy Mode-Guided Percutaneous Radiologic Gastrostomy (PRG)—Comparison of Interventional Parameters and Billing
Authors: Brönnimann Michael P. , Kulagowska Jagoda , Gebauer Bernhard , Auer Timo A. , Collettini Federico , Schnapauff Dirk , Magyar Christian T. J. , Komarek Alois , Krokidis Miltiadis , Heverhagen Johannes T.
Published: August 2024

Background: This study investigated and compared the efficacy, safety, radiation exposure, and financial compensation of two modalities for percutaneous radiologic gastrostomy (PRG): multislice computed tomography biopsy mode (MS-CT BM)-guided and fluoroscopy-guided (FPRG). The aim was to provide insights into optimizing radiologically assisted gastrostomy procedures. Methods: We conducted a retrospective analysis of PRG procedures performed at a single center from January 2018 to January 2024. The procedures were divided into two groups based on the imaging modality used. We compared patient demographics, intervention parameters, complication rates, and procedural times. Financial compensation was evaluated based on the tariff structure for outpatient medical services in Switzerland (TARMED). Statistical differences were determined using Fisher’s exact test and the Mann–Whitney U test. Results: The study cohort included 133 patients: 55 with MS-CT BM-PRG and 78 with FPRG. The cohort comprised 35 women and 98 men, with a mean age of 64.59 years (±11.91). Significant differences were observed between the modalities in effective dose (MS-CT BM-PRG: 10.95 mSv ± 11.43 vs. FPRG: 0.169 mSv ± 0.21, p < 0.001) and procedural times (MS-CT BM-PRG: 41.15 min ± 16.14 vs. FPRG: 28.71 min ± 16.03, p < 0.001). Major complications were significantly more frequent with FPRG (10% vs. 0% in MS-CT BM-PRG, p = 0.039, φ = 0.214). A higher single-digit number of MS-CT BM-guided PRG was required initially to reduce procedure duration by 10 min. Financial comparison revealed that only 4% of MS-CT BM-guided PRGs achieved reimbursement equivalent to the most frequent comparable examination, according to TARMED. Conclusions: Based on our experience from a retrospective, single-center study, the execution of a PRG using MS-CT BM, as opposed to FPRG, is currently justified in challenging cases despite a lower incidence of major complications. However, further well-designed prospective multicenter studies are needed to determine the efficacy, safety, and cost-effectiveness of these two modalities.
Distinguishing Childhood Asthma Exacerbations from Stable Asthma: The Utility of Inflammatory White Blood Cell Biomarkers
Authors: Asseri Ali Alsuheel
Published: August 2024

Background: Asthma is a chronic inflammatory condition characterized by episodes of acute asthma exacerbations (AAEs), in addition to chronic airway inflammation, which has a huge impact on both the affected patients and their parents. The main objective of this study was to explore the utility of available white-blood-cell-derived inflammatory markers in diagnosing AAEs and identifying children at risk for severe exacerbations requiring admission to the pediatric intensive care unit (PICU). Methods: This study was a retrospective cohort study. The medical records of 128 children diagnosed with asthma exacerbation and 131 children with stable asthma between the ages of 2 and 12 years were reviewed. Results: A total of 259 participants were enrolled. Children with AAE demonstrated significantly higher white blood cell counts (WBC: 10.0 ± 4.2 × 103/μL vs. 7.1 ± 2.2 × 103/μL, p < 0.001), absolute neutrophil counts (ANC: 7398.5 ± 4600 cells/μL vs. 2634.8 ± 1448 cells/μL, p < 0.001), and neutrophil-to-lymphocyte ratios (NLR: 7.0 ± 6.8 vs. 0.9 ± 0.7, p < 0.001) but significantly lower absolute lymphocyte counts (ALC: 1794.1 ± 1536 × 103/μL vs. 3552.9 ± 1509 × 103/μL, p < 0.001). Interestingly, blood eosinophil count displayed an opposite trend: children with stable asthma had significantly more eosinophils compared to those experiencing an exacerbation (370.1 ± 342.7 cells/mm3 vs. 0.9 ± 1.9 cells/mm3, p < 0.001). Two criteria that are indicative of AAE were identified: NLR values greater than 1.2, with good discriminative ability (area under the curve [AUC] 0.90; 95% confidence interval [CI] 0.85–0.94; sensitivity 82.5%; specificity 79.5%), and ANC values exceeding 3866, with moderate discriminative ability (AUC 0.86; 95% CI 0.81–0.91; sensitivity 75.0%; specificity 82.3%). Moreover, a comparative analysis of these markers (NLR, ANC, PLR, WBC, AEC, and ALC) in patients with AAE did not demonstrate significant differences between those requiring PICU admission and those who did not require it. Conclusions: This study contributes two major findings. The first is that NLR, ANC, WBC, and PLR are significantly higher in AAE patients compared to those with stable asthma. The second is that children with stable asthma have higher AEC and ALC levels compared to those with AAE. Furthermore, this study has revealed that the studied markers (NLR, ANC, PLR, WBC, AEC, and ALC) did not differentiate between AAE patients requiring PICU admission and those managed in the general ward, suggesting a need for alternative predictive factors.
Artificial Intelligence (AI) Applications for Point of Care Ultrasound (POCUS) in Low-Resource Settings: A Scoping Review
Authors: Kim Seungjun , Fischetti Chanel , Guy Megan , Hsu Edmund , Fox John , Young Sean D.
Published: August 2024

Advancements in artificial intelligence (AI) for point-of-care ultrasound (POCUS) have ushered in new possibilities for medical diagnostics in low-resource settings. This review explores the current landscape of AI applications in POCUS across these environments, analyzing studies sourced from three databases—SCOPUS, PUBMED, and Google Scholars. Initially, 1196 records were identified, of which 1167 articles were excluded after a two-stage screening, leaving 29 unique studies for review. The majority of studies focused on deep learning algorithms to facilitate POCUS operations and interpretation in resource-constrained settings. Various types of low-resource settings were targeted, with a significant emphasis on low- and middle-income countries (LMICs), rural/remote areas, and emergency contexts. Notable limitations identified include challenges in generalizability, dataset availability, regional disparities in research, patient compliance, and ethical considerations. Additionally, the lack of standardization in POCUS devices, protocols, and algorithms emerged as a significant barrier to AI implementation. The diversity of POCUS AI applications in different domains (e.g., lung, hip, heart, etc.) illustrates the challenges of having to tailor to the specific needs of each application. By separating out the analysis by application area, researchers will better understand the distinct impacts and limitations of AI, aligning research and development efforts with the unique characteristics of each clinical condition. Despite these challenges, POCUS AI systems show promise in bridging gaps in healthcare delivery by aiding clinicians in low-resource settings. Future research endeavors should prioritize addressing the gaps identified in this review to enhance the feasibility and effectiveness of POCUS AI applications to improve healthcare outcomes in resource-constrained environments.
Mindful Eating Approaches to Cardiometabolic Risk Factors: Systematic Review and Meta-Analysis of Intervention Studies
Authors: Iaccarino Idelson Paola , D’Elia Lanfranco , Strazzullo Pasquale
Published: August 2024

Mindful eating is the direct application of mindfulness to eating related issues. This approach has been developed to reduce suffering due to food and body image, to improve the capacity to follow the stimuli of hunger and satiety and to minimize the use of food as psychological compensation. The aim of this review is to analyze the results of clinical trials adopting a mindful eating approach to address cardiometabolic risk factors partly related to dysfunctional eating behavior. The selection of literature included articles published until 31 December 2023. The inclusion criteria were controlled randomized clinical trials, an intervention duration ≥4 weeks and indication of a clinical outcome. Fourteen studies were included. The sample sizes ranged from 18 to 194 participants, and the interventions lasted between 4 and 24 weeks. Their effects on body weight, BMI, waist circumference, serum glucose, glycated hemoglobin and C-reactive protein were assessed using a meta-analysis. Mindful eating was as effective as other recognized types of interventions regarding the clinical outcomes examined, but in many cases, they were more effective in terms of factors which may lead to improvement over a longer period. In particular, our analysis showed that mindful eating effectively reduced suffering related to food and body image.
8-OxodG: A Potential Biomarker for Chronic Oxidative Stress Induced by High-LET Radiation
Authors: Kumar Kamendra , Fornace Albert J. , Suman Shubhankar
Published: August 2024

Oxidative stress-mediated biomolecular damage is a characteristic feature of ionizing radiation (IR) injury, leading to genomic instability and chronic health implications. Specifically, a dose- and linear energy transfer (LET)-dependent persistent increase in oxidative DNA damage has been reported in many tissues and biofluids months after IR exposure. Contrary to low-LET photon radiation, high-LET IR exposure is known to cause significantly higher accumulations of DNA damage, even at sublethal doses, compared to low-LET IR. High-LET IR is prevalent in the deep space environment (i.e., beyond Earth’s magnetosphere), and its exposure could potentially impair astronauts’ health. Therefore, the development of biomarkers to assess and monitor the levels of oxidative DNA damage can aid in the early detection of health risks and would also allow timely intervention. Among the recognized biomarkers of oxidative DNA damage, 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-OxodG) has emerged as a promising candidate, indicative of chronic oxidative stress. It has been reported to exhibit differing levels following equivalent doses of low- and high-LET IR. This review discusses 8-OxodG as a potential biomarker of high-LET radiation-induced chronic stress, with special emphasis on its potential sources, formation, repair mechanisms, and detection methods. Furthermore, this review addresses the pathobiological implications of high-LET IR exposure and its association with 8-OxodG. Understanding the association between high-LET IR exposure-induced chronic oxidative stress, systemic levels of 8-OxodG, and their potential health risks can provide a framework for developing a comprehensive health monitoring biomarker system to safeguard the well-being of astronauts during space missions and optimize long-term health outcomes.
Genome Assembly and Annotation of Vietnamese Rice Lines with Diverse Life-Cycle Durations
Authors: Franco Ortega Sara , Thi Thuy Luu , Trong Khanh Nguyen , Thu Hang Le , Thi Yen Tran , Thi Ngoan Le , Thi Thanh Le , Thien Thanh Pham , Ouyang Xinhao , Tao Wenjing , James Sally , Gilbert Lesley , Davis Amanda M. , Gomez Leonardo D. , Harper Andrea L. , McQueen-Mason Simon J. , Xuan Tu Duong , Davis Seth Jon
Published: August 2024

This study begins by examining phenotypic variations in field growth among four parental Vietnamese rice lines, consisting of two Indica (PD211/GL37) and two Japonica (J23/SRA2-1) cultivars, which differ in life-cycle durations. Their phenotypic observations revealed both similarities and differences in growth patterns and field responses, setting the stage for further genomic investigation. We then focused on the sequencing and de novo genome assembly of these lines using high-coverage Illumina sequencing and achieving pseudochromosome assemblies ranging between 379 Mbp and 384 Mbp. The assemblies were further enhanced by annotation processes, designating between 44,427 and 48,704 gene models/genome. A comparative genomic analysis revealed that the Japonica varieties (J23/SRA2-1) exhibited more genetic similarity than the Indica varieties (PD211/GL37). From this, a phylogenetic analysis on the phytochrome C (phyC) gene distinctly positions the Indica and Japonica lines within their respective clades, affirming their genetic diversity and lineage accuracy. These genomic resources will pave the way for identifying quantitative trait loci (QTLs) critical for developing rice cultivars with shorter life cycles, thus enhancing resilience to adverse climatic impacts in Vietnam. This study provides a foundational step towards leveraging genomic data for rice breeding programs aimed at ensuring food security in the face of climate change.
ERRT-GA: Expert Genetic Algorithm with Rapidly Exploring Random Tree Initialization for Multi-UAV Path Planning
Authors: Xu Hong , Niu Zijing , Jiang Bo , Zhang Yuhang , Chen Siji , Li Zhiqiang , Gao Mingke , Zhu Miankuan
Published: August 2024

In unmanned aerial vehicle (UAV) path planning, evolutionary algorithms are commonly used due to their ability to handle high-dimensional spaces and wide generality. However, traditional evolutionary algorithms have difficulty with population initialization and may fall into local optima. This paper proposes an improved genetic algorithm (GA) based on expert strategies, including a novel rapidly exploring random tree (RRT) initialization algorithm and a cross-variation process based on expert guidance and the wolf pack search algorithm. Experimental results on baseline functions in different scenarios show that the proposed RRT initialization algorithm improves convergence speed and computing time for most evolutionary algorithms. The expert guidance strategy helps algorithms jump out of local optima and achieve suboptimal solutions that should have converged. The ERRT-GA is tested for task assignment, path planning, and multi-UAV conflict detection, and it shows faster convergence, better scalability to high-dimensional spaces, and a significant reduction in task computing time compared to other evolutionary algorithms. The proposed algorithm outperforms most other methods and shows great potential for UAV path planning problems.