bluuebunny/crossref_metadata_2025 huggingface dataset:
The association between pediatric injury risks and parenting behaviours
Authors: Jaques M. L. , Weaver T. L. , Weaver N. L. , Willoughby L.
Published: October 2017

Background

Unintentional injuries are the leading cause of death in children ages 1–18 years. Many of these injuries to young children occur in their own homes. Although research has explored injury risk prevention strategies, historically, much of this research has focused on environmental changes and teaching safety practices. Currently, there appears to be a gap in current research exploring how parenting influences children's risk of injury.

Methods

Mothers (n = 119) of children 5 years and younger were recruited from a paediatric clinic as a part of a larger study and completed measures of parenting challenges, developmentally sensitive parenting, child neglect, parental efficacy, and risk of potential injury situations. Hierarchical logistic regression was used to explore the extent to which developmentally insensitive parenting behaviours put parents at higher risk for behaviours that lead to unintentional injury in children and whether developmentally sensitive parenting behaviours protects children from injury. The association between demographic characteristics and injury risk behaviours was also examined.

Results

Parents who reported more frequent insensitive parenting behaviours (i.e., yelling, spanking, and putting child in time out) were more likely to report putting their child in an incorrect car seat or taking their child out of a car seat while the car is still moving. In addition, younger parents were at greater risk of storing cleaners and medications unsafely.

Conclusion

Results from this study highlight the importance of supporting younger mothers and educating parents on effective parenting strategies when trying to prevent unintentional injury risks.
Scaling Theory of Mind in a Small‐Scale Society: A Case Study From Vanuatu
Authors: Dixson Henry G. W. , Komugabe‐Dixson Aimée F. , Dixson Barnaby J. , Low Jason
Published: October 2017

Although theory of mind (ToM) is argued to emerge between 3 and 5 years of age, data from non‐Western, small‐scale societies suggest diversity. Deeper investigations into these settings are warranted. In the current study, over 400 Melanesian children from Vanuatu (range = 3–14 years), growing up in either urban or rural remote environments, completed culturally tailored ToM batteries. Results show a marked delay in false belief (FB) performance, particularly among participants from rural villages. By further investigating a diverse range of concepts beyondFB, we illustrate two unique cultural sequences for a suite of mental state concepts among urban and rural ni‐Vanuatu children. Implications for social and cultural influences on the development of ToM are discussed.
Dentists’ opinions on using digital technologies in dental practice
Authors: van der Zande Marieke M. , Gorter Ronald C. , Bruers Josef J. M. , Aartman Irene H. A. , Wismeijer Daniel
Published: October 2017

Objectives

To investigate which opinions among dentists are associated with level of technology use, when characteristics of the dentist and dental practice, as well as motivating work aspects are taken into account.

Methods

A total of 1000 general dental practitioners in the Netherlands received a questionnaire on digital technologies they use, opinions on using technologies and related motivating work aspects. Questions were derived from expert interviews, the Dentists’ Experienced Job Resources Scale and literature on technology implementation. Technology use was measured as the number of technologies used, and divided into three technology user groups: low (using 0‐4 technologies, mostly administrative and radiographic technologies), intermediate (using 5‐7, more varied technologies) and high technology users (using 8‐12, including more innovative diagnostic technologies). Opinions on technology use and motivating work aspects were analysed using principal components analysis (PCA) and exploratory factor analysis. Scores on all components and factors were calculated for each respondent by computing the mean of all valid responses on the underlying items. Differences in these scale scores on opinions among the technology user groups were assessed using one‐way analysis of variance and Kruskal–Wallis tests. A multiple linear regression analysis assessed the association of scale scores about opinions on technology use with the sum of technologies used, taking into account motivating work aspects and characteristics of the dentist and dental practice.

Results

The response rate was 31%. Dentists who were high technology users perceived technologies as yielding more improvements in quality of care, adding more value to the dental practice and being easier to use, than low technology users. High technology users thought technologies added more value to their work and they reported higher skills and resources. They also focused more on technologies and thought these are more ready to use than low technology users. High technology users derived more motivation from “Immediate results” and “Craftsmanship” than low technology users. Personal and practice characteristics, motivating work aspects, and the opinion scales “Focus” and “Added value to dentist” explained 50% of the variance in the number of technologies a dentist uses.

Conclusion

Opinions on digital technologies among dentists and motivating work aspects vary with level of technology use. Being more focused on technologies and perceiving a higher added value from using them are associated with using more digital dental technologies, when taking into account motivating work aspects and characteristics of the dentist and dental practice.
MicroRNA-126 deficiency enhanced the activation and function of CD4+T cells by elevating IRS-1 pathway
Authors: Chu F , Hu Y , Zhou Y , Guo M , Lu J , Zheng W , Xu H , Zhao J , Xu L
Published: October 2017

Recent evidence has shown that microRNA-126 (miR-126) has been involved in the development and function of immune cells, which contributed to the pathogenesis of related clinical diseases. However, the potential role of miR-126 in the development and function of CD4+ T cells remains largely unknown. Here we first found that the activation and proliferation, as well as the expression of interferon (IFN)-γ, of CD4+T cells from miR-126 knock-down (KD) mice using the miRNA-sponge technique were enhanced significantly in vitro, compared with those in CD4+ T cells from wild-type (WT) mice. To monitor further the possible effect of miR-126 deficiency on the function of CD4+ T cells in vivo, we used dextran sulphate sodium (DSS)-induced murine model of acute autoimmune colitis and found that miR-126 deficiency could elevate the pathology of colitis. Importantly, the proportion of CD4+ T cells in splenocytes increased significantly in miR-126KD mice. Moreover, the expression levels of CD69 and CD44 on CD4+ T cells increased significantly and the expression level of CD62L decreased significantly. Of note, adoptive cell transfer assay showed that the pathology of colitis was more serious in carboxyfluorescein succinimidyl ester (CFSE)-labelled miR-126KD CD4+ T cell-transferred group, compared with that in the CFSE-labelled WT CD4+ T cells transferred group. Consistently, the expression levels of CD69 and CD44 on CFSE+ cells increased significantly. Furthermore, both the proliferation and IFN-γ secretion of CFSE+ cells also increased significantly in the CFSE-labelled miR-126KD CD4+ T cell-transferred group. Mechanistic evidence showed that the expression of insulin receptor substrate 1 (IRS-1), as a functional target of miR-126, was elevated in CD4+ T cells from miR-126KD mice, accompanied by altered transduction of the extracellular regulated kinase, protein B (AKT) and nuclear factor kappa B (NF-κB) pathway. Our data revealed a novel role in which miR-126 was an intrinsic regulator in the function of CD4+ T cells, which provided preliminary basis for exploring further the role of miR-126 in the development, function of CD4+ T cells and related clinical diseases.
Rational Inference of Beliefs and Desires From Emotional Expressions
Authors: Wu Yang , Baker Chris L. , Tenenbaum Joshua B. , Schulz Laura E.
Published: October 2017

We investigated people's ability to infer others’ mental states from their emotional reactions, manipulating whether agents wanted, expected, and caused an outcome. Participants recovered agents’ desires throughout. When the agent observed, but did not cause the outcome, participants’ ability to recover the agent's beliefs depended on the evidence they got (i.e., her reaction only to the actual outcome or to both the expected and actual outcomes; Experiments 1 and 2). When the agent caused the event, participants’ judgments also depended on the probability of the action (Experiments 3 and 4); when actions were improbable given the mental states, people failed to recover the agent's beliefs even when they saw her react to both the anticipated and actual outcomes. A Bayesian model captured human performance throughout (rs ≥ .95), consistent with the proposal that people rationally integrate information about others’ actions and emotional reactions to infer their unobservable mental states.
Redefining “Learning” in Statistical Learning: What Does an Online Measure Reveal About the Assimilation of Visual Regularities?
Authors: Siegelman Noam , Bogaerts Louisa , Kronenfeld Ofer , Frost Ram
Published: October 2017

From a theoretical perspective, most discussions of statistical learning (SL) have focused on the possible “statistical” properties that are the object of learning. Much less attention has been given to defining what “learning” is in the context of “statistical learning.” One major difficulty is that SL research has been monitoring participants’ performance in laboratory settings with a strikingly narrow set of tasks, where learning is typically assessed offline, through a set of two‐alternative‐forced‐choice questions, which follow a brief visual or auditory familiarization stream. Is that all there is to characterizing SL abilities? Here we adopt a novel perspective for investigating the processing of regularities in the visual modality. By tracking online performance in a self‐paced SL paradigm, we focus on the trajectory of learning. In a set of three experiments we show that this paradigm provides a reliable and valid signature of SL performance, and it offers important insights for understanding how statistical regularities are perceived and assimilated in the visual modality. This demonstrates the promise of integrating different operational measures to our theory of SL.
Development and evaluation of ‘Pure Rush’: An online serious game for drug education
Authors: Stapinski Lexine A. , Reda Bill , Newton Nicola C. , Lawler Siobhan , Rodriguez Daniel , Chapman Catherine , Teesson Maree
Published: October 2017

Introduction and Aims

Learning is most effective when it is active, enjoyable and incorporates feedback. Past research demonstrates that serious games are prime candidates to utilise these principles, however the potential benefits of this approach for delivering drug education are yet to be examined in Australia, a country where drug education in schools is mandatory.

Design and Methods

The serious game ‘Pure Rush’ was developed across three stages. First, formative consultation was conducted with 115 students (67% male, aged 15–17 years), followed by feasibility and acceptability testing of a prototype of the game (n = 25, 68% male). In the final stage, 281 students (62% female, aged 13–16 years) were randomly allocated to receive a lesson involving Pure Rush or an active control lesson. The lessons were compared in terms of learning outcomes, lesson engagement and future intentions to use illicit drugs.

Results

Students enjoyed playing Pure Rush, found the game age‐appropriate and the information useful to them. Both the Pure Rush and the active control were associated with significant knowledge increase from pre to post‐test. Among females, multi‐level mixed‐effects regression showed knowledge gain was greater in the Pure Rush condition compared to control (β = 2.36, 95% confidence interval 0.36–4.38). There was no evidence of between condition differences in lesson engagement or future intentions to use illicit drugs.

Discussion and Conclusions

Pure Rush is an innovative online drug education game that is well received by students and feasible to implement in schools. [Stapinski LA, Reda B, Newton NC, Lawler S, Rodriguez D, Chapman C, Teesson M. Development and evaluation of ‘Pure Rush’: An online serious game for drug education. Drug Alcohol Rev 2017]
Care maps for children with medical complexity
Authors: Adams Sherri , Nicholas David , Mahant Sanjay , Weiser Natalie , Kanani Ronik , Boydell Katherine , Cohen Eyal
Published: October 2017

Aim

Children with medical complexity require multiple providers and services to keep them well and at home. A care map is a patient/family‐created diagram that pictorially maps out this complex web of services. This study explored what care maps mean for families and healthcare providers to inform potential for clinical use.

Method

Parents (n=15) created care maps (hand drawn n=10 and computer‐generated n=5) and participated in semi‐structured interviews about the process of developing care maps and their perceived impact. Healthcare providers (n=30) reviewed the parent‐created care maps and participated in semi‐structured interviews. Data were analysed for themes and emerging theory using a grounded theory analytical approach.

Results

Data analysis revealed 13 overarching themes that were further categorized into three domains: features (characteristics of care maps), functions (what care maps do), and emerging outcomes (benefits of care map use). These domains further informed a definition and a theoretical model of how care maps work.

Interpretation

Our findings suggest that care maps may be a way of supporting patient‐ and family‐centred care by graphically identifying and integrating experiences of the family as well as priorities for moving forward.

What this paper adds

Care maps were endorsed as a useful tool by families and providers.

They help healthcare providers better understand parental priorities for care.

Parents can create care maps to demonstrate the complex burden of care.

They are a unique visual way to incorporate narrative medicine into practice.
Disentangling vegetation and climate as drivers of Australian vertebrate richness
Authors: Coops Nicholas C. , Rickbeil Gregory J. M. , Bolton Douglas K. , Andrew Margaret E. , Brouwers Niels C.
Published: October 2017

Determining drivers of species richness is recognised as highly complex, involving many synergies and interactions. We examine the utility of newly available remote sensing representations of vegetation productivity and vegetation structure to examine drivers of species richness at continental and regional scales. We related richness estimates derived from stacked species distribution models for birds, mammals, amphibians, and reptiles to estimates of actual and potential evapotranspiration (AET and PET), forest structure, and forest productivity across Australia as a whole as well as by bioclimatic zones. We used structural equation modeling to partition correlations between climate energy and vegetation attributes and their subsequent associations with species richness. Continentally, vertebrate richness patterns were strongly related to patterns of energy availability. Richness of amphibians, mammals, and birds were positively associated with AET. However, reptile richness was most strongly associated with PET. Regionally, forest structure and productivity associations with bird, mammal, and amphibian richness were strongest. Again, reptile richness associated most strongly with PET. Our results suggest that a hierarchy of drivers of broad‐scale vertebrate richness patterns exist (reptiles excluded): 1) climate energy is most important at the continental scale; next, 2) vegetation productivity and vegetation structure are most important at the regional scale; except 3) at low extremes of climate energy when energy becomes limiting.
The effect of fragment area on site‐level biodiversity
Authors: Phillips Helen R. P. , Halley John M. , Urbina‐Cardona J. Nicolas , Purvis Andy
Published: October 2017

Habitat fragmentation accompanies habitat loss, and drives additional biodiversity change; but few global biodiversity models explicitly analyse the effects of both fragmentation and loss. Here we propose and test the hypothesis that, as fragment area increases, species density (the number of species in a standardised plot) will scale with an exponent given by the difference between the exponents of the species–area relationships for islands (z ~ 0.25) and in contiguous habitat (z ~ 0.15), and test whether scaling varies between land uses. We also investigate the scaling of overall abundance and rarefaction‐based richness, as some mechanisms make different predictions about how fragment area should affect them. The relevant data from the taxonomically and geographically broad PREDICTS database were used to model the three diversity measures, testing their scaling with fragment area and whether the scaling exponent varied among land uses (primary forest, secondary forest, plantation forest, cropland and pasture). In addition, the consistency of the response of species density to fragment area was tested across three well represented taxa (Magnoliopsida, Hymenoptera and ‘herptiles’). Species density and total abundance showed area‐scaling exponents of 0.07 and 0.16, respectively, and these exponents did not vary significantly among land uses; rarefaction‐based richness by contrast did not increase consistently with area. These results suggest that the area‐scaling of species density is driven by the area‐scaling of total abundance, with additive edge effects (species moving into the small fragments from the surroundings) opposing – but not fully overcoming – the effect of fragment area on overall density of individuals. The interaction between fragment area and higher taxon (plants, vertebrates and invertebrates), which remained in the rarefied richness model, indicates that mechanisms may vary among groups.
Improved species‐occurrence predictions in data‐poor regions: using large‐scale data and bias correction with down‐weighted Poisson regression and Maxent
Authors: El‐Gabbas Ahmed , Dormann Carsten F.
Published: October 2017

Species distribution modelling (SDM) has become an essential method in ecology and conservation. In the absence of survey data, the majority of SDMs are calibrated with opportunistic presence‐only data, incurring substantial sampling bias. We address the challenge of correcting for sampling bias in the data‐sparse situations. We modelled the relative intensity of bat records in their entire range using three modelling algorithms under the point‐process modelling framework (GLMs with subset selection, GLMs fitted with an elastic‐net penalty, and Maxent). To correct for sampling bias, we applied model‐based bias correction by incorporating spatial information on site accessibility or sampling efforts. We evaluated the effect of bias correction on the models’ predictive performance (AUC and TSS), calculated on spatial‐block cross‐validation and a holdout data set. When evaluated with independent, but also sampling‐biased test data, correction for sampling bias led to improved predictions. The predictive performance of the three modelling algorithms was very similar. Elastic‐net models have intermediate performance, with slight advantage for GLMs on cross‐validation and Maxent on hold‐out evaluation. Model‐based bias correction is very useful in data‐sparse situations, where detailed data are not available to apply other bias correction methods. However, bias correction success depends on how well the selected bias variables describe the sources of bias. In this study, accessibility covariates described bias in our data better than the effort covariate, and their use led to larger changes in predictive performance. Objectively evaluating bias correction requires bias‐free presence–absence test data, and without them the real improvement for describing a species’ environmental niche cannot be assessed.
The importance of phenotypic plasticity and local adaptation in driving intraspecific variability in thermal niches of marine macrophytes
Authors: King Nathan G. , McKeown Niall J. , Smale Dan A. , Moore Pippa J.
Published: October 2017

Climate change is driving the redistribution of species at a global scale and documenting and predicting species' responses to warming is a principal focus of contemporary ecology. When interpreting and predicting their responses to warming, species are generally treated as single homogenous physiological units. However, local adaptation and phenotypic plasticity can result in intraspecific differences in thermal niche. Therefore, population loss may also not only occur from trailing edges. In species with low dispersal capacity this will have profound impacts for the species as a whole, as local population loss will not be offset by immigration of warm tolerant individuals. Recent evidence from terrestrial forests has shown that incorporation of intraspecific variation in thermal niche is vital to accurately predicting species responses to warming. However, marine macrophytes (i.e. seagrasses and seaweeds) that form some of the world's most productive and diverse ecosystems have not been examined in the same context. We conducted a literature review to determine how common intraspecific variation in thermal physiology is in marine macrophytes. We find that 90% of studies identified (n = 42) found clear differences in thermal niche between geographically separated populations. Therefore, non‐trailing edge populations may also be vulnerable to future warming trends and given their limited dispersal capacity, such population loss may not be offset by immigration. We also explore how next generation sequencing (NGS) is allowing unprecedented mechanistic insight into plasticity and adaptation. We conclude that in the ‘genomic era’ it may be possible to link understanding of plasticity and adaptation at the genetic level through to changes in populations providing novel insights on the redistribution of populations under future climate change.
Functional compensation by insular scavengers: the relative contributions of vertebrates and invertebrates vary among islands
Authors: Sugiura Shinji , Hayashi Masakazu
Published: October 2017

When the extinction of some species results in loss of ecosystem functions, other species may be able to compensate for this loss. Functional compensation has recently been observed on islands where species are likely to become extinct; however, few studies have analysed functional compensation by scavengers in insular environments. Here, we investigated the ecosystem functions of vertebrate and invertebrate scavengers on Honshu, the main island of Japan, and offshore islands (four of the Oki Islands). The Oki Islands lack several native vertebrate scavengers (raccoon dogs, boars, martens, and foxes) that are abundant on Honshu. We experimentally placed mouse carcasses on the forest floors of Honshu (12 sites) and the Oki Islands (7 sites on Dōgo, 4 sites on Nishinoshima, 4 sites on Nakanoshima, and 3 sites on Chiburijima) in September–October 2014 and 2015. Nearly all of the mouse carcasses (95–100%) placed on the forest floor disappeared within 1 week. However, the relative importance of vertebrate and invertebrate scavengers varied among islands. Vertebrate and invertebrate scavengers contributed equally on Honshu (carcass removal by vertebrates, 47%), whereas vertebrate scavengers rarely contributed to carcass removal on three of the Oki Islands (carcass removal by vertebrates: 17% on Dōgo, 30% on Nishinoshima, and 5% on Nakanoshima). Consequently, invertebrate scavengers (Nicrophorus burying beetles) functionally compensated for the low species diversity of vertebrate scavengers on the three islands (carcass burial by Nicrophorus beetles, 65–95%). However, on the small island of Chiburijima, introduced raccoon dogs contributed to nearly all of the removals (93%), suggesting that introduced scavengers can compensate for the functional reduction. This functional compensation by scavengers may help stabilise the functions and/or services of scavengers in island environments.
Extinction‐driven changes in frugivore communities on oceanic islands
Authors: Heinen Julia H. , van Loon E. Emiel , Hansen Dennis M. , Kissling W. Daniel
Published: October 2017

Global change and human expansion have resulted in many species extinctions worldwide, but the geographic variation and determinants of extinction risk in particular guilds still remain little explored. Here, we quantified insular extinctions of frugivorous vertebrates (including birds, mammals and reptiles) across 74 tropical and subtropical oceanic islands within 20 archipelagos worldwide and investigated extinction in relation to island characteristics (island area, isolation, elevation and climate) and species’ functional traits (body mass, diet and ability to fly). Out of the 74 islands, 33 islands (45%) have records of frugivore extinctions, with one third (mean: 34%, range: 2–100%) of the pre‐extinction frugivore community being lost. Geographic areas with more than 50% loss of pre‐extinction species richness include islands in the Pacific (within Hawaii, Cook Islands and Tonga Islands) and the Indian Ocean (Mascarenes, Seychelles). The proportion of species richness lost from original pre‐extinction communities is highest on small and isolated islands, increases with island elevation, but is unrelated to temperature or precipitation. Large and flightless species had higher extinction probability than small or volant species. Across islands with extinction events, a pronounced downsizing of the frugivore community is observed, with a strong extinction‐driven reduction of mean body mass (mean: 37%, range: –18–100%) and maximum body mass (mean: 51%, range: 0–100%). The results document a substantial trophic downgrading of frugivore communities on oceanic islands worldwide, with a non‐random pattern in relation to geography, island characteristics and species’ functional traits. This implies severe consequences for ecosystem processes that depend on mutualistic plant–animal interactions, including ecosystem dynamics that result from the dispersal of large‐seeded plants by large‐bodied frugivores. We suggest that targeted conservation and rewilding efforts on islands are needed to halt the defaunation of large and non‐volant seed dispersers and to restore frugivore communities and key ecological interactions.
Applying species distribution models to caves and other subterranean habitats
Authors: Mammola Stefano , Leroy Boris
Published: October 2017

Over the last two decades there has been an exponential increase in the use of correlative species distribution models (SDMs) to address a variety of topics in ecology, biogeography, evolution, and conservation biology. Conversely, the use of these statistical methods to study the potential distribution of subterranean organisms has lagged behind, relative to their above‐ground (epigean) counterparts. The reason for this is possibly related to a number of peculiarities of subterranean systems, which pose important limits, but also opportunities, for these correlative models. The aim of this forum is to explore the caveats that need to be made when generalizing these statistical techniques to caves and other subterranean habitats. We focus on the typical bias in spatial datasets of cave‐dwelling species, and provide advice for selecting the model calibration area. In parallel, we discuss the potential use of different large scale surface variables to represent the subterranean condition. A more widespread adoption of these statistical techniques in subterranean biology is highly attractive and has great potential in broadening our knowledge on a variety of ecological topics, especially in the fields of climate change and biodiversity conservation. Their use would especially benefit the study of the biogeographic patterns of subterranean fauna and the impact of past and future climate change on subterranean ecosystems.
Generalist predator's niche shifts reveal ecosystem changes in an experimentally fragmented landscape
Authors: Resasco Julian , Tuff Kika T. , Cunningham Saul A. , Melbourne Brett A. , Hicks Andrew L. , Newsome Seth D. , Davies Kendi F.
Published: October 2017

Habitat fragmentation can alter the trophic structure of communities and environmental conditions, thus driving changes in biodiversity and ecosystem functions. Quantifying niches of generalist predators can reveal how fragmentation alters ecosystems. In a habitat fragmentation experiment, we used stable isotopes of a generalist predator skink to test predictions from spatial theory on trophic structure and to quantify abiotic changes associated with fragmentation among continuous forest, fragments, and matrix habitats. We predicted that in fragments and the matrix, isotopic niches would shift due to decreases in skink trophic positions (δ15N) from reductions in trophic structure of arthropod food webs and abiotic changes over time (δ13C) relative to continuous forest. Contrary to theoretical predictions, we did not find evidence of reductions in trophic structure with fragmentation. In fact, skink δ15N values were higher in the matrix and fragments than continuous forest, likely due to changes in distributions of a detritivorous prey species. In addition, δ13C values in the matrix decreased over years since fragmentation due to abiotic changes associated with matrix tree maturation. We show how isotopic niches are influenced by fragmentation via shifts in biotic and abiotic processes. The potential for either or both spatial and abiotic effects of fragmentation present a challenge for theory to better predict ecological changes in fragmented landscapes.
Limited effect of organic matter on soil available water capacity
Authors: Minasny B. , McBratney A. B.
Published: October 2017

Soil water‐holding capacity is an important component of the water and energy balances of the terrestrial biosphere. It controls the rate of evapotranspiration, and is a key to crop production. It is widely accepted that the available water capacity in soil can be improved by increasing organic matter content. However, the increase in amount of water that is available to plants with an increase in organic matter is still uncertain and may be overestimated. To clarify this issue, we carried out a meta‐analysis from 60 published studies and analysed large databases (more than 50 000 measurements globally) to seek relations between organic carbon (OC) and water content at saturation, field capacity, wilting point and available water capacity. We show that the increase in organic carbon in soil has a small effect on soil water content. A 1% mass increase in soil OC (or 10 g C kg−1 soil mineral), on average, increases water content at saturation, field capacity, wilting point and available water capacity by: 2.95, 1.61, 0.17 and 1.16 mm H2O 100 mm soil−1, respectively. The increase is larger in sandy soils, followed by loams and is least in clays. Overall the increase in available water capacity is very small; 75% of the studies reported had values between 0.7 and 2 mm 100 mm−1 with an increase of 10 g C kg−1 soil. Compared with reported annual rates of carbon sequestration after the adoption of conservation agricultural systems, the effect on soil available water is negligible. Thus, arguments for sequestering carbon to increase water storage are questionable. Conversely, global warming may cause losses in soil carbon, but the effects on soil water storage and its consequent impact on hydrological cycling might be less than thought previously.

Highlights

We investigated how available water capacity can be increased with a 1% increase in soil organic carbon.

We analysed data from 60 published studies and global databases with more than 50 000 measurements.

The increase in organic carbon in soil has a small effect on soil water retention.

A 1% mass increase in soil OC on average increased available water capacity by 1.16%, volumetrically.
RNA‐seq analysis of Lgr6+ stem cells and identification of an Lgr6 isoform
Authors: van de Glind Gerline C. , Bastidas Torres Armando N. , Zoutman Willem H. , van der Zeeuw Sander A. J. , Kiełbasa Szymon M. , de Gruijl Frank R. , Tensen Cornelis P.
Published: October 2017

We studied Lgr6+ stem cells in experimental UV carcinogenesis in hairless mice. For further characterization through RNA‐seq, these stem cells were isolated by FACS from transgenic hairless mice bearing an EGFP‐Ires‐CreERT2 reporter cassette inserted into exon 1 of the Lgr6 gene (purity confirmed by human ERT2 expression). Between Lgr6/EGFP+ and Lgr6/EGFP− basal cells (Tg/wt), 682 RNAs were differentially expressed, indicating stemness and expression of cancer‐related pathways in Lgr6/EGFP+ cells. We discovered that suspected “Lgr6 null” mice (Tg/Tg) expressed RNA of an Lgr6 isoform (delta‐Lgr6, lacking 74 N‐terminal aa) which could be functional and explain the lack of a phenotype.
Crystal structure of Mycobacterium tuberculosis VapC20 toxin and its interactions with cognate antitoxin, VapB20, suggest a model for toxin–antitoxin assembly
Authors: Deep Amar , Kaundal Soni , Agarwal Sakshi , Singh Ramandeep , Thakur Krishan Gopal
Published: October 2017

VapBCs, virulence‐associated proteins, are the most abundant type II toxin–antitoxin (TA) systems in prokaryotes. Under normal conditions, toxin and antitoxin interact to form a heterooctameric complex, which upon binding to operator sites, inhibits their own expression. Under stress conditions, the VapB antitoxin is degraded by cellular proteases to release a free VapC toxin, which in turn inhibits cell growth mainly by targeting protein translation. However, the intermediate steps involved in the assembly of the heterooctameric complex have not been resolved. Here, we report a 1.75 Å resolution crystal structure of VapC20, a Sarcin‐Ricin loop cleaving toxin from type II TA system of Mycobacterium tuberculosis. Using analytical ultracentrifugation (AUC) studies, we show that VapC20 exists as a homodimer in solution. The structural analysis of VapC homologs further suggests that VapCs form homodimers. We demonstrate that VapC20 is an obligate homodimer, and its self‐association is critical for its folding and activity. Surface plasmon resonance experiments suggest that VapC20 interacts with its cognate antitoxin VapB20 to form a stable complex with nanomolar affinity. A high association rate coupled with a very slow dissociation rate ensures minimal toxicity under normal growth conditions. AUC studies reveal that VapB20 also exists as a homodimer in solution and further associates with VapC20 dimers to form heterotetramers and heterooctamers in a concentration‐dependent manner. The results presented here provide valuable insights into the assembly of VapBC family of toxins which is essential for their function and regulation.

Database

Structural data are available in the PDB under the accession numbers 5WZF and 5WZ4.
Escherichia coli DNA polymerase I can disrupt G‐quadruplex structures during DNA replication
Authors: Teng Fang‐Yuan , Hou Xi‐Miao , Fan San‐Hong , Rety Stephane , Dou Shuo‐Xing , Xi Xu‐Guang
Published: October 2017

Non‐canonical four‐stranded G‐quadruplex (G4) DNA structures can form in G‐rich sequences that are widely distributed throughout the genome. The presence of G4 structures can impair DNA replication by hindering the progress of replicative polymerases (Pols), and failure to resolve these structures can lead to genetic instability. In the present study, we combined different approaches to address the question of whether and how Escherichia coli Pol I resolves G4 obstacles during DNA replication and/or repair. We found that E. coli Pol I‐catalyzed DNA synthesis could be arrested by G4 structures at low protein concentrations and the degree of inhibition was strongly dependent on the stability of the G4 structures. Interestingly, at high protein concentrations, E. coli Pol I was able to overcome some kinds of G4 obstacles without the involvement of other molecules and could achieve complete replication of G4 DNA. Mechanistic studies suggested that multiple Pol I proteins might be implicated in G4 unfolding, and the disruption of G4 structures requires energy derived from dNTP hydrolysis. The present work not only reveals an unrealized function of E. coli Pol I, but also presents a possible mechanism by which G4 structures can be resolved during DNA replication and/or repair in E. coli.
Decoupling the direct and indirect effects of climate on plant litter decomposition: Accounting for stress‐induced modifications in plant chemistry
Authors: Suseela Vidya , Tharayil Nishanth
Published: October 2017

Decomposition of plant litter is a fundamental ecosystem process that can act as a feedback to climate change by simultaneously influencing both the productivity of ecosystems and the flux of carbon dioxide from the soil. The influence of climate on decomposition from a postsenescence perspective is relatively well known; in particular, climate is known to regulate the rate of litter decomposition via its direct influence on the reaction kinetics and microbial physiology on processes downstream of tissue senescence. Climate can alter plant metabolism during the formative stage of tissues and could shape the final chemical composition of plant litter that is available for decomposition, and thus indirectly influence decomposition; however, these indirect effects are relatively poorly understood. Climatic stress disrupts cellular homeostasis in plants and results in the reprogramming of primary and secondary metabolic pathways, which leads to changes in the quantity, composition, and organization of small molecules and recalcitrant heteropolymers, including lignins, tannins, suberins, and cuticle within the plant tissue matrix. Furthermore, by regulating metabolism during tissue senescence, climate influences the resorption of nutrients from senescing tissues. Thus, the final chemical composition of plant litter that forms the substrate of decomposition is a combined product of presenescence physiological processes through the production and resorption of metabolites. The changes in quantity, composition, and localization of the molecular construct of the litter could enhance or hinder tissue decomposition and soil nutrient cycling by altering the recalcitrance of the lignocellulose matrix, the composition of microbial communities, and the activity of microbial exo‐enzymes via various complexation reactions. Also, the climate‐induced changes in the molecular composition of litter could differentially influence litter decomposition and soil nutrient cycling. Compared with temperate ecosystems, the indirect effects of climate on litter decomposition in the tropics are not well understood, which underscores the need to conduct additional studies in tropical biomes. We also emphasize the need to focus on how climatic stress affects the root chemistry as roots contribute significantly to biogeochemical cycling, and on utilizing more robust analytical approaches to capture the molecular composition of tissue matrix that fuel microbial metabolism.
Hide and seek – Infection rates and alkaloid concentrations of Epichloë festucae var. lolii in Lolium perenne along a land‐use gradient in Germany
Authors: König J. , Fuchs B. , Krischke M. , Mueller M. J. , Krauss J.
Published: October 2017

The common forage grass Lolium perenne has evolved with the systemic fungal endophyte Epichloë festucae var. lolii. The endophyte provides herbivore resistance to the grass due to defensive alkaloids, some of which are toxic to grazing livestock. In this field study, we determine whether distribution of the endophyte‐grass association changes along a land‐use intensity gradient on 87 managed grasslands in three German regions. Endophyte infections were detected in 66% of the studied sites and infection rates within infected sites ranged from 1% to 95%. Alkaloid concentrations of lolitrem B (vertebrate toxin) exceeded the toxicity thresholds in 50 (14%) of 351 infected plants and of peramine (invertebrate deterrent/toxin) in 12 (3%) of 351 plants. Infection rates and alkaloid concentrations were not significantly affected by land‐use intensity and region, but alkaloid concentrations were higher in summer compared to spring. We conclude that risks for livestock intoxication are currently low, as (i) average alkaloid concentrations per grassland were always below toxicity thresholds and as (ii) none of the grasslands was dominated by L. perenne. We suggest avoidance of grass monocultures in Europe to keep intoxication risks for livestock low; we also recommend regular examination of seeds and grasslands, as seed producers might accidentally distribute infected seeds, and as climate warming might further enhance the distribution of Epichloë endophytes in European grasslands.
Utility of Phox2b immunohistochemical stain in neural crest tumours and non‐neural crest tumours in paediatric patients
Authors: Warren Mikako , Matsuno Ryosuke , Tran Henry , Shimada Hiroyuki
Published: October 2017

Aims

This study evaluated the utility of Phox2b in paediatric tumours. Previously, tyrosine hydroxylase (TH) was the most widely utilised sympathoadrenal marker specific for neural crest tumours with neuronal/neuroendocrine differentiation. However, its sensitivity is insufficient. Recently Phox2b has emerged as another specific marker for this entity.

Methods and results

Phox2b immunohistochemistry (IHC) was performed on 159 paediatric tumours, including (group 1) 65 neural crest tumours with neuronal differentiation [peripheral neuroblastic tumours (pNT)]: 15 neuroblastoma undifferentiated (NB‐UD), 10 NB poorly differentiated (NB‐PD), 10 NB differentiating (NB‐D), 10 ganglioneuroblastoma intermixed (GNBi), 10 GNB nodular (GNBn) and 10 ganglioneuroma (GN); (group 2) 23 neural crest tumours with neuroendocrine differentiation [pheochromocytoma/paraganglioma (PCC/PG)]; (group 3) 27 other neural crest tumours including one composite rhabdomyosarcoma/neuroblastoma; and (group 4) 44 non‐neural crest tumours. TH IHC was performed on groups 1, 2 and 3. Phox2b was expressed diffusely in pNT (n = 65 of 65), strongly in NB‐UD and NB‐PD and with less intensity in NB‐D, GNB and GN. Diffuse TH was seen in all NB‐PD, NB‐D, GNB and GN, but nine of 15 NB‐UD and a nodule in GNBn did not express TH (n = 55 of 65). PCC/PG expressed diffuse Phox2b (n = 23 of 23) and diffuse TH, except for one tumour (n = 22 of 23). In composite rhabdomyosarcoma, TH was expressed only in neuroblastic cells and Phox2b was diffusely positive in neuroblastic cells and focally in rhabdomyosarcoma. All other tumours were negative for Phox2b (n = none of 44).

Conclusion

Phox2b was a specific and sensitive marker for pNT and PCC/PG, especially useful for identifying NB‐UD often lacking TH. Our study also presented a composite rhabdomyosarcoma/neuroblastoma of neural crest origin.
Dental visiting behaviours among primary schoolchildren: Application of the health belief model
Authors: Lee C‐Y , Ting C‐C , Wu J‐H , Lee K‐T , Chen H‐S , Chang Y‐Y
Published: October 2017

Objectives

This study aimed to develop and validate a new instrument based on the health belief model and to use the instrument to investigate the determinants of regular dental attendance among primary schoolchildren.

Methods

A cross‐sectional study was conducted using a newly developed measurement scale based on the HBM, 4 health‐promoting schools participated in the study and 958 students studying in grades 4–6 completed the questionnaire. The psychometric properties of the instrument were analysed, and a path analysis model was used to identify the determinants of regular dental attendance.

Results

The instrument had good internal consistency (Cronbach's α = 0.826–0.925) and a factor structure identical to HBM. Overall, the schoolchildren's health beliefs on caries treatment were positive. The determinants of regular dental visit were school location (β = −0.13), mother's education level (β = 0.15), susceptibility (β = −0.18) and barriers (β = −0.11).

Conclusion

This study provided evidence that HBM is applicable to children's dental visiting behaviour and their health beliefs towards adherence to caries treatment. Although children had a positive attitude towards dental visits, environmental obstacles would interfere with dental visits. The newly developed instrument could be used to identify high‐risk children and help design oral health interventions for these children. Moreover, policy makers should increase the accessibility of dental resources to enhance the utilization of dental care among schoolchildren.
Determinants of how individuals choose, eat and waste: Providing common ground to enhance sustainable food consumption out‐of‐home
Authors: Lorenz Bettina A. , Langen Nina
Published: October 2017

The transformation of food consumption in wealthy economies is regarded as an essential measure to reach global sustainability goals. However, existing policies and research activities to change food consumption in the increasingly relevant out‐of‐home sector relate to a wide set of options on how to influence behaviours and may be criticized to lack a general focus. Against this background, our study provides a structured review of the existing research body on the determinants of individuals’ food choices and food consumption out‐of‐home. It structures the various research approaches and findings for 110 selected papers according to a general ecological framework where personal, social and environmental determinants for food behaviours are considered. By providing a collective overview and linking results for different behavioural aspects and settings, this study supports a more general understanding of consumer food behaviour in out‐of‐home settings. Consequently, it also provides a means to identify research gaps and to suggest relevant aspects for future research to draw from the combination of findings and to enhance sustainability in food consumption.
Diagnosis and management of diaper dermatitis in infants with emphasis on skin microbiota in the diaper area
Authors: Šikić Pogačar Maja , Maver Uroš , Marčun Varda Nataša , Mičetić‐Turk Dušanka
Published: October 2017

Diaper dermatitis is the most common inflammatory skin eruption within the diaper area in infants and toddlers. Although it rarely causes problems for prolonged periods of time, it causes considerable distress for both infants and parents. Diapered skin is exposed to friction, excessive hydration, varying pH, and is in constant contact with urine and feces, both of which are highly irritant to the skin. The three most common types of diaper dermatitis include chaffing dermatitis, irritant contact dermatitis, and diaper candidiasis. However, a variety of other skin conditions may manifest in the diaper area. Candida albicans and Staphylococcus aureus are most frequently isolated from the affected area and are thought to have a predominant role in diaper dermatitis. The review includes an overview of the epidemiology, etiology, and common skin microbiota in the diaper area. State‐of‐the‐art strategies for diagnosis, management, and prevention of diaper dermatitis are also included. Appropriate skin care can help in the prevention of diaper dermatitis and can aid the reparation of the skin. This review discusses common causes, skin microbiota within the diapered area, differential diagnosis, and finally possible prevention and treatment recommendations. Recent findings have proven that the key to efficient eradication of irritant diaper dermatitis lies in its prevention.