bluuebunny/crossref_metadata_2025 huggingface dataset:
Shapeshifting the Scottish Borders: A Geopoetic Dance of Place
Authors: Pençak Claire
Published: August 2019

In this paper, I unite dance theory and practice and geopoetics in order to reflect on edges, peripheries and borders in a geographic region, the Scottish Borders, where the dominant cultural narrative is and has historically been based on rivalry. I draw here on the writing of the Scottish poet-philosopher Kenneth White, the practices of specific dancers and choreographers and on relational accounts of place and more-than-human perspectives. Rather than ‘sense of place’, my interest is in sensing place and thinking through sites. Threaded throughout are descriptions of perception practices exploring woodland, stone and riverways, which take the reader into the more experiential realm of embodied knowing. These passages are an invitation to be present with more-than-human others, to be in contact with the vitality of materials and to allow for being shaped, rather than being the shaping force. The intention is to bring different bodies of knowledge into contact as a way of revealing other vocabularies within place, which suggest alternative cultural narratives and help create the conditions for place—making a more collaborative, ethical and less anthropocentric endeavour, open to the influence and organising principles of the more-than-human.
An Electrocatalytic Screen-Printed Amperometric Sensor for the Selective Measurement of Thiamine (Vitamin B1) in Food Supplements
Authors: Smart Amy , Westmacott Kelly L. , Crew Adrian , Doran Olena , Hart John P.
Published: August 2019

An electrocatalytic screen-printed sensor has been investigated for the measurement of the biologically important biomolecule vitamin B1 (thiamine) for the first time in food supplements. Under basic conditions, the vitamin was converted to its electrochemically active thiolate anion species. It was shown that an electrocatalytic oxidation reaction occurred with the screen-printed carbon electrode containing the mediator cobalt phthalocyanine (CoPC-SPCE). This had the advantage of producing an analytical response current at an operating potential of 0 V vs. Ag/AgCl compared to +0.34 V obtained with plain SPCEs. This resulted in improved selectivity and limit of detection. Detailed studies on the underlying mechanism occurring with the sensor are reported in this paper. A linear response was obtained between 0.1 and 20 µg mL−1, which was suitable for the quantification of the vitamin in two commercial products containing vitamin B1. The mean recovery for a multivitamin tablet with a declared content of 5 mg was 101% (coefficient of variation (CV) of 9.6%). A multivitamin drink, which had a much lower concentration of vitamin B1 (0.22 mg/100 mL), gave a mean recovery of 93.3% (CV 7.2%). These results indicate that our sensor holds promise for quality control of food supplements and other food types.
The Biological and Clinical Relevance of Inhibitor of Growth (ING) Genes in Non-Small Cell Lung Cancer
Authors: Smolle Elisabeth , Fink-Neuboeck Nicole , Lindenmann Joerg , Smolle-Juettner Freyja , Pichler Martin
Published: August 2019

Carcinogenic mutations allow cells to escape governing mechanisms that commonly inhibit uncontrolled cell proliferation and maintain tightly regulated homeostasis between cell death and survival. Members of the inhibition of growth (ING) family act as tumor suppressors, governing cell cycle, apoptosis and cellular senescence. The molecular mechanism of action of ING genes, as well as their anchor points in pathways commonly linked to malignant transformation of cells, have been studied with respect to a variety of cancer specimens. This review of the current literature focuses specifically on the action mode of ING family members in lung cancer. We have summarized data from in vitro and in vivo studies, highlighting the effects of varying levels of ING expression in cancer cells. Based on the increasing insight into the function of these proteins, the use of ING family members as clinically useful biomarkers for lung cancer detection and prognosis will probably become routine in everyday clinical practice.
Cancer-Derived VEGF-C Increases Chemokine Production in Lymphatic Endothelial Cells to Promote CXCR2-Dependent Cancer Invasion and MDSC Recruitment
Authors: Chen Jing-Yi , Lai You-Syuan , Chu Pei-Yi , Chan Shih-Hsuan , Wang Lu-Hai , Hung Wen-Chun
Published: August 2019

Breast cancer-derived vascular endothelial growth factor-C (VEGF-C) has been shown to enhance lymphangiogenesis in lymph nodes to accelerate cancer metastasis. However, the remodeling of lymph node microenvironments by VEGF-C remains elusive. By in vivo selection, we established a subline (named as “LC”) with strong lymphatic tropism and high VEGF-C expression from the human MDA-MB-231 breast cancer cell line. Co-culture with LC cells or treatment with LC-conditioned medium upregulated the expression of CXC chemokines in lymphatic endothelial cells (LECs), which could be inhibited by pre-incubation with VEGF-C-neutralizing antibodies and VEGFR3 inhibitors. The chemokines produced by LECs enhanced recruitment of myeloid-derived suppressor cells (MDSCs) to tumor-draining and distant lymph nodes in tumor-bearing mice. Treatment with a CXCR2 inhibitor after tumor cell inoculation dramatically decreased the number of MDSCs in lymph nodes, suggesting the importance of the chemokine/CXCR2 signaling axis in MDSC recruitment. In addition, LEC-released chemokines also stimulated the expression of serum amyloid A1 (SAA1) in cancer cells, enhancing their lymphatic invasion by increasing VE-cadherin phosphorylation, junction disruption, and vascular permeability of LECs. Clinical sample validation confirmed that SAA1 expression was associated with increased lymph node metastasis. Collectively, we reveal a novel mechanism by which cancer cell-derived VEGF-C remodels lymphovascular microenvironments by regulating chemokine production in LECs to promote cancer invasion and MDSC recruitment. Our results also suggest that inhibition of CXCR2 is effective in treating lymphatic metastasis.
Novel Strategies for Cancer Treatment: Highlights from the 55th IACR Annual Conference
Authors: Charmsaz Sara , Collins Denis , Perry Antoinette , Prencipe Maria
Published: August 2019

While conventional cancer treatments, such as surgery, radiotherapy and chemotherapy, have been combined for decades in an effort to treat cancer patients, the emergence of novel fields of cancer research have led to a renewed interest in combining conventional treatments with more innovative approaches. The realisation that cancer progression is not exclusively due to changes in the cancer epithelial cells, but also involves changes in the tumour microenvironment, has opened new avenues for combination treatments. Here we discuss the use of combination therapies presented at the 55th Irish Association for Cancer Research (IACR) Annual Conference, highlighting examples of novel therapeutic strategies which, combined with conventional therapies, may greatly enhance not only the overall outcome for patients, but also the quality of life for cancer survivors. Among the novel treatment strategies, immune metabolism, epigenetic therapies and physical exercise are presented. In addition, novel technologies in the field of precision medicine, which will be useful to discover new therapeutics and to stratify patients for combination treatments, are also discussed.
Catalytic Dehydration of Ethanol over WOx Nanoparticles Supported on MFI (Mobile Five) Zeolite Nanosheets
Authors: Kim Haneul , Numan Muhammad , Jo Changbum
Published: August 2019

Ethylene can be synthesized in a renewable manner by dehydrating bioethanol over supported metal oxide nanoparticle catalysts. Here, a series of nanoparticulate tungsten oxides supported on MFI (Mobil five) zeolite nanosheets was prepared at different W loadings (1 to 6 mol %) using the incipient wetness method and investigated with respect to the ability to catalyze the dehydration of ethanol. The resulting samples were characterized by X-ray diffraction, electron microscopy, N2 isotherms, X-ray absorption fine structures, and by the temperature-programmed desorption of NH3. The results obtained showed that WOx nanoparticles were homogeneously distributed over the entire void space of nanosheet samples up to a loading of 2 mol %, after which large WOx nanoparticles with needle-like morphology were formed on the surface of the zeolite nanosheet beyond 2mol%. The number of acid sites increased with WOx loading and, as a result, EtOH conversion progressively increased with WOx loading up to 6 mol %. At reaction temperatures of >390 °C, homogeneously distributed WOx nanoparticles showed slightly higher ethylene selectivity than nano-needle structured WOx. However, nano-needle structured WOx exhibited greater catalytic stability. In terms of ethylene yield over 8 h, needle-like WOx nanoparticles were found to be more suitable for the acid-catalyzed dehydration of ethanol than small-sized WOx nanoparticles.
DeliveryCoin: An IDS and Blockchain-Based Delivery Framework for Drone-Delivered Services
Authors: Ferrag Mohamed Amine , Maglaras Leandros
Published: August 2019

In this paper, we propose an intrusion detection system (IDS) and Blockchain-based delivery framework, called DeliveryCoin, for drone-delivered services. The DeliveryCoin framework consists of four phases, including system initialization phase, creating the block, updating the blockchain, and intrusion detection phase. To achieve privacy-preservation, the DeliveryCoin framework employs hash functions and short signatures without random oracles and the Strong Diffie–Hellman (SDH) assumption in bilinear groups. To achieve consensus inside the blockchain-based delivery platform, we introduce a UAV-aided forwarding mechanism, named pBFTF. We also propose an IDS system in each macro eNB (5G) for detecting self-driving network attacks as well as false transactions between self-driving nodes. Furthermore, extensive simulations are conducted, and results confirm the efficiency of our proposed DeliveryCoin framework in terms of latency of blockchain consensus and accuracy.
Twenty Years of Quantum State Teleportation at the Sapienza University in Rome
Authors: De Martini Francesco , Sciarrino Fabio
Published: August 2019

Quantum teleportation is one of the most striking consequence of quantum mechanics and is defined as the transmission and reconstruction of an unknown quantum state over arbitrary distances. This concept was introduced for the first time in 1993 by Charles Bennett and coworkers, it has then been experimentally demonstrated by several groups under different conditions of distance, amount of particles and even with feed forward. After 20 years from its first realization, this contribution reviews the experimental implementations realized at the Quantum Optics Group of the University of Rome La Sapienza.
Beta Distribution-Based Cross-Entropy for Feature Selection
Authors: Dai Weixing , Guo Dianjing
Published: August 2019

Analysis of high-dimensional data is a challenge in machine learning and data mining. Feature selection plays an important role in dealing with high-dimensional data for improvement of predictive accuracy, as well as better interpretation of the data. Frequently used evaluation functions for feature selection include resampling methods such as cross-validation, which show an advantage in predictive accuracy. However, these conventional methods are not only computationally expensive, but also tend to be over-optimistic. We propose a novel cross-entropy which is based on beta distribution for feature selection. In beta distribution-based cross-entropy (BetaDCE) for feature selection, the probability density is estimated by beta distribution and the cross-entropy is computed by the expected value of beta distribution, so that the generalization ability can be estimated more precisely than conventional methods where the probability density is learnt from data. Analysis of the generalization ability of BetaDCE revealed that it was a trade-off between bias and variance. The robustness of BetaDCE was demonstrated by experiments on three types of data. In the exclusive or-like (XOR-like) dataset, the false discovery rate of BetaDCE was significantly smaller than that of other methods. For the leukemia dataset, the area under the curve (AUC) of BetaDCE on the test set was 0.93 with only four selected features, which indicated that BetaDCE not only detected the irrelevant and redundant features precisely, but also more accurately predicted the class labels with a smaller number of features than the original method, whose AUC was 0.83 with 50 features. In the metabonomic dataset, the overall AUC of prediction with features selected by BetaDCE was significantly larger than that by the original reported method. Therefore, BetaDCE can be used as a general and efficient framework for feature selection.
Entropic Characterization of Quantum States with Maximal Evolution under Given Energy Constraints
Authors: Majtey Ana P. , Valdés-Hernández Andrea , Maglione César G. , Plastino Angel R.
Published: August 2019

A measure D [ t 1 , t 2 ] for the amount of dynamical evolution exhibited by a quantum system during a time interval [ t 1 , t 2 ] is defined in terms of how distinguishable from each other are, on average, the states of the system at different times. We investigate some properties of the measure D showing that, for increasing values of the interval’s duration, the measure quickly reaches an asymptotic value given by the linear entropy of the energy distribution associated with the system’s (pure) quantum state. This leads to the formulation of an entropic variational problem characterizing the quantum states that exhibit the largest amount of dynamical evolution under energy constraints given by the expectation value of the energy.
A Multi-Agent Social Gamification Model to Guide Sustainable Urban Photovoltaic Panels Installation Policies
Authors: Olszewski Robert , Pałka Piotr , Wendland Agnieszka , Kamiński Jacek
Published: August 2019

The paper presents a holistic and quantitative model of social gamification in a smart city, which is likely to stimulate the photovoltaic panels installation. The coupling of multi-agent systems, GIS tools, demographic data, and a spatial knowledge base made it possible to develop and calibrate a computable model of social interaction in a “model smart city,” as well as to quantitatively evaluate the deployment of photovoltaic panels. It also enabled the analysis of factors affecting the efficiency of this process, e.g., the photovoltaic potential of solar roofs, the ownership of buildings, the type of building development, the level of social trust, institutional and social incentives, and the development of an information society. The devised model is tested on the city of Warsaw, utilizing spatial and descriptive data provided by city authorities.
Multi-Objective Optimization of Off-Grid Hybrid Renewable Energy Systems in Buildings with Prior Design-Variable Screening
Authors: Conti Paolo , Lutzemberger Giovanni , Schito Eva , Poli Davide , Testi Daniele
Published: August 2019

This work presents an optimization strategy and the cost-optimal design of an off-grid building served by an energy system involving solar technologies, thermal and electrochemical storages. Independently from the multi-objective method (e.g., utility function) and algorithm used (e.g., genetic algorithms), the optimization of this kind of systems is typically characterized by a high-dimensional variables space, computational effort and results uncertainty (e.g., local minimum solutions). Instead of focusing on advanced optimization tools to handle the design problem, the dimension of the full problem has been reduced, only considering the design variables with a high “effect” on the objective functions. An off-grid accommodation building is presented as test case: the original six-variable design problem consisting of about 300,000 possible configurations is reduced to a two-variable problem, after the analysis of 870 Monte Carlo simulations. The new problem includes only 220 possible design alternatives with a clear benefit for the multi-objective optimization algorithm. The energy-economy Pareto frontiers obtained by the original and the reduced problems overlap, showing the validity of the proposed methodology. The No-RES (no renewable energy sources) primary energy consumption can be reduced up to almost 0 kWh/(m2yr) and the net present value (NPV) after 20 years can reach 70 k€ depending on the number of photovoltaic panels and electrochemical storage size. The reduction of the problem also allows for a plain analysis of the results and the drawing of handy decision charts to help the investor/designer in finding the best design according to the specific investment availability and target performances. The configurations on the Pareto frontier are characterized by a notable electrical overproduction and a ratio between the two main design variables that goes from 4 to 8 h. A sensitivity analysis to the unitary price of the electrochemical storage reveals the robustness of the sizing criterion.
Life Cycle Assessment of Fuel Cell Vehicles Considering the Detailed Vehicle Components: Comparison and Scenario Analysis in China Based on Different Hydrogen Production Schemes
Authors: Chen Yisong , Hu Xu , Liu Jiahui
Published: August 2019

Numerous studies concerning the life cycle assessment of fuel cell vehicles (FCVs) have been conducted. However, little attention has been paid to the life cycle assessment of an FCV from the perspective of the detailed vehicle components. This work conducts the life cycle assessment of Toyota Mirai with all major components considered in a Chinese context. Both the vehicle cycle and the fuel cycle are included. Both comprehensive resources and energy consumption and comprehensive environmental emissions of the life cycles are investigated. Potential environmental impacts are further explored based on CML 2001 method. Then different hydrogen production schemes are compared to obtain the most favorable solution. To explore the potential of the electrolysis, the scenario analysis of the power structure is conducted. The results show that the most mineral resources are consumed in the raw material acquisition stage, the most fossil energy is consumed in the use stage and global warming potential (GWP) value is fairly high in all life cycle stages of Toyota Mirai using electrolyzed hydrogen. For hydrogen production schemes, the scenario analysis indicates that simply by optimizing the power structure, the environmental impact of the electrolysis remains higher than other schemes. When using the electricity from hydropower or wind power, the best choice will be the electrolysis.
Influence of Cambial Age and Axial Height on the Spatial Patterns of Xylem Traits in Catalpa bungei, a Ring-Porous Tree Species Native to China
Authors: Li Shan , Li Xin , Link Roman , Li Ren , Deng Liping , Schuldt Bernhard , Jiang Xiaomei , Zhao Rongjun , Zheng Jingming , Li Shuang , Yin Yafang
Published: August 2019

Studying how cambial age and axial height affects wood anatomical traits may improve our understanding of xylem hydraulics, heartwood formation and axial growth. Radial strips were collected from six different heights (0–11.3 m) along the main trunk of three Manchurian catalpa (Catalpa bungei) trees, yielding 88 samples. In total, thirteen wood anatomical vessel and fiber traits were observed usinglight microscopy (LM) and scanning electron microscopy (SEM), and linear models were used to analyse the combined effect of axial height, cambial age and their interaction. Vessel diameter differed by about one order of magnitude between early- and latewood, and increased significantly with both cambial age and axial height in latewood, while it was positively affected by cambial age and independent of height in earlywood. Vertical position further had a positive effect on earlywood vessel density, and negative effects on fibre wall thickness, wall thickness to diameter ratio and length. Cambial age had positive effects on the pit membrane diameter and vessel element length, while the annual diameter growth decreased with both cambial age and axial position. In contrast, early- and latewood fiber diameter were unaffected by both cambial age and axial height. We further observed an increasing amount of tyloses from sapwood to heartwood, accompanied by an increase of warty layers and amorphous deposits on cell walls, bordered pit membranes and pit apertures. This study highlights the significant effects of cambial age and vertical position on xylem anatomical traits, and confirms earlier work that cautions to take into account xylem spatial position when interpreting wood anatomical structures, and thus, xylem hydraulic functioning.
Application of Image Analysis for the Identification of Prehistoric Ceramic Production Technologies in the North Caucasus (Russia, Bronze/Iron Age)
Authors: Park Ki Suk , Milke Ralf , Rybacki Erik , Reinhold Sabine
Published: August 2019

The recent advances in microscopy and scanning techniques enabled the image analysis of archaeological objects in a high resolution. From the direct measurements in images, shapes and related parameters of the structural elements of interest can be derived. In this study, image analysis in 2D/3D is applied to archaeological ceramics, in order to obtain clues about the ceramic pastes, firing and shaping techniques. Images were acquired by the polarized light microscope, scanning electron microscopy (SEM) and 3D micro X-ray computed tomography (µ-CT) and segmented using Matlab. 70 ceramic sherds excavated at Ransyrt 1 (Middle-Late Bronze Age) and Kabardinka 2 (late Bronze–early Iron Age), located in in the North Caucasian mountains, Russia, were investigated. The size distribution, circularity and sphericity of sand grains in the ceramics show site specific difference as well as variations within a site. The sphericity, surface area, volume and Euler characteristic of pores show the existence of various pyrometamorphic states between the ceramics and within a ceramic. Using alignments of pores and grains, similar pottery shaping techniques are identified for both sites. These results show that the image analysis of archaeological ceramics can provide detailed information about the prehistoric ceramic production technologies with fast data availability.
Geospatial Analysis of Mass-Wasting Susceptibility of Four Small Catchments in Mountainous Area of Miyun County, Beijing
Authors: Cao Chen , Chen Jianping , Zhang Wen , Xu Peihua , Zheng Lianjing , Zhu Chun
Published: August 2019

Driven by the pull of gravity, mass-wasting comprises all of the sedimentary processes related to remobilization of sediments deposited on slopes, including creep, sliding, slumping, flow, and fall. It is vital to conduct mass-wasting susceptibility mapping, with the aim of providing decision makers with management advice. The current study presents two individual data mining methods—the frequency ratio (FR) and information value model (IVM) methods—to map mass-wasting susceptibility in four catchments in Miyun County, Beijing, China. To achieve this goal, nine influence factors and a mass-wasting inventory map were used and produced, respectively. In this study, 71 mass-wasting locations were investigated in the field. Of these hazard locations, 70% of them were randomly selected to build the model, and the remaining 30% of the hazard locations were used for validation. Finally, a receiver operating characteristic (ROC) curve was used to assess the mass-wasting susceptibility maps produced by the above-mentioned models. Results show that the FR had a higher concordance and spatial differentiation, with respective values of 0.902 (area under the success rate) and 0.883 (area under the prediction rate), while the IVM had lower values of 0.865 (area under the success rate) and 0.855 (area under the prediction rate). Both proposed methodologies are useful for general planning and evaluation purposes, and they are shown to be reasonable models. Slopes of 6–21° were the most common thresholds that controlled occurrence of mass-wasting. Farmland terraces were mainly composed of gravel, mud, and clay, which are more prone to mass-wasting. Mass-wasting susceptibility mapping is feasible and potentially highly valuable. It could provide useful information in support of environmental health policies.
Development and Validation of an Interdisciplinary Worker’s Health Approach Instrument (IWHAI)
Authors: Viterbo Lilian Monteiro Ferrari , Dinis Maria Alzira Pimenta , Costa André Santana , Vidal Diogo Guedes
Published: August 2019

The present study aimed to develop and validate an Interdisciplinary Worker’s Health Approach Instrument (IWHAI). The development stage comprised a group of 10 professionals, including physicians, nurses, nutritionists, dentists and physical educators, as well as a judges’ committee, composed by 19 recognized experts in the area of worker’s health (WH). For the validation of the IWHAI, the Spearman’s correlation coefficient (rs) was calculated, the factor analysis to the instrument was applied, and the Cronbach’s alpha (α) and the Intraclass correlation coefficient (ICC) were calculated. The IWHAI was structured in five dimensions, integrating 43 health indicators, on a scale of 0–4, totalling 215 sub-indices with closed response coding. The instrument was validated with a Kappa coefficient (KAPPA) (k), with excellent agreement for all attributes, i.e., k = 0.88 for applicability, k = 0.80 for clarity and k = 0.82 for relevance. p > 0.05 results reveal moderate to strong positive correlations between some variables, i.e., pests, vectors and air quality/drinking water quality (rs = 0.69). A total of 14 components of the factor analysis, explaining 62.6% of the data variance, were extracted. α value is considered moderate to high, α = 0.61, the ICC value also being considered moderate to high, with ICC = 0.61. The IWHAI is considered validated, constituting a technological innovation for an interdisciplinary approach in the field of WH, enabling the prevention and integral promotion of health.
End-Users and Caregivers’ Involvement in Health Interventional Research Carried Out in Geriatric Facilities: A Systematic Review
Authors: Ahouah Mathieu , Rothan-Tondeur Monique
Published: August 2019

Public involvement (PI) is of great interest. However, little is known about this topic in the design, development, and/or implementation of health interventions in geriatric facilities. This study aimed to provide a critical overview of the involvement of caregivers and end-users in interventions in these facilities, based on Rifkin’s analytical framework. This systematic review, supplemented by a questionnaire to the corresponding authors, covered non-drug intervention reports targeting nurses, doctors, residents, and their relatives. Articles were published in Pubmed, Medline, Scopus, and Cinahl, from January 2016 to April 2018. Ninety-seven articles were included. The review shows a low level or partial PI in geriatric facilities where it exists. These results are further supported by the authors’ responses to the questionnaire. PI remains uncommon in geriatric institutions and consists of a consumerist model, suggesting the need for improved practices. More efforts are needed to experiment with recommendations to meet the challenges of PI and enhance the public ownership of interventions. The protocol was registered on Prospero under the number CRD42018098504.
The Characteristics of Polycyclic Aromatic Hydrocarbons in Different Emission Source Areas in Shenyang, China
Authors: Yang Lu , Suzuki Genki , Zhang Lulu , Zhou Quanyu , Zhang Xuan , Xing Wanli , Shima Masayuki , Yoda Yoshiko , Nakatsubo Ryohei , Hiraki Takatoshi , Sun Baijun , Fu Wenhua , Qi Hongye , Hayakawa Kazuichi , Toriba Akira , Tang Ning
Published: August 2019

Particulate matter (PM) was collected in three different areas, SY-1, SY-2, and SY-3, in Shenyang, China, during the warm and cold seasons from 2012 to 2014. SY-1 was located beside a thermal power plant, far from the central area. SY-2 was near a coal heating boiler on the main road, close to the central area. SY-3 was on the main road, without fixed emission sources. Nine PM-bound polycyclic aromatic hydrocarbons (PAHs) were analyzed. The results showed that the mean concentration of total PAHs was higher in the cold season (92.6–316 ng m−3) than in the warm season (18.4–32.2 ng m−3). Five- and six-ring PAHs occupied a large percentage at all sites in the warm season, and four-ring PAHs were the dominant components in the cold season. Several diagnostic PAH ratios indicated that the main sources of PAHs in Shenyang in the warm and cold seasons were not only coal burning but also vehicle emission. In this study, we suggest that a benzo[a]pyrene/benzo[ghi]perylene ratio ([BaP]/[BgPe]) of 0.6 was a useful indicator to speculate the relative significance of coal burning and vehicle exhaust. Although the Shenyang government has undertaken actions to address air pollution, the PM and PAH concentrations did not decrease significantly compared to those in our previous studies. The cancer risk calculated from the BaP equivalent total concentration at all three sites in the warm and cold seasons exceeded the acceptable limit established by the US EPA.
Recent Progress in the Regeneration of Spinal Cord Injuries by Induced Pluripotent Stem Cells
Authors: Csobonyeiova Maria , Polak Stefan , Zamborsky Radoslav , Danisovic Lubos
Published: August 2019

Regeneration of injuries occurring in the central nervous system, particularly spinal cord injuries (SCIs), is extremely difficult. The complex pathological events following a SCI often restrict regeneration of nervous tissue at the injury site and frequently lead to irreversible loss of motor and sensory function. Neural stem/progenitor cells (NSCs/NPCs) possess neuroregenerative and neuroprotective features, and transplantation of such cells into the site of damaged tissue is a promising stem cell-based therapy for SCI. However, NSC/NPCs have mostly been induced from embryonic stem cells or fetal tissue, leading to ethical concerns. The pioneering work of Yamanaka and colleagues gave rise to the technology to induce pluripotent stem cells (iPSCs) from somatic cells, overcoming these ethical issues. The advent of iPSCs technology has meant significant progress in the therapy of neurodegenerative disease and nerve tissue damage. A number of published studies have described the successful differentiation of NSCs/NPCs from iPSCs and their subsequent engraftment into SCI animal models, followed by functional recovery of injury. The aim of this present review is to summarize various iPSC- NPCs differentiation methods, SCI modelling, and the current status of possible iPSC- NPCs- based therapy of SCI.
Role of Carbonic Anhydrases and Inhibitors in Acid–Base Physiology: Insights from Mathematical Modeling
Authors: Occhipinti Rossana , Boron Walter F.
Published: August 2019

Carbonic anhydrases (CAs) catalyze a reaction fundamental for life: the bidirectional conversion of carbon dioxide (CO2) and water (H2O) into bicarbonate (HCO3−) and protons (H+). These enzymes impact numerous physiological processes that occur within and across the many compartments in the body. Within compartments, CAs promote rapid H+ buffering and thus the stability of pH-sensitive processes. Between compartments, CAs promote movements of H+, CO2, HCO3−, and related species. This traffic is central to respiration, digestion, and whole-body/cellular pH regulation. Here, we focus on the role of mathematical modeling in understanding how CA enhances buffering as well as gradients that drive fluxes of CO2 and other solutes (facilitated diffusion). We also examine urinary acid secretion and the carriage of CO2 by the respiratory system. We propose that the broad physiological impact of CAs stem from three fundamental actions: promoting H+ buffering, enhancing H+ exchange between buffer systems, and facilitating diffusion. Mathematical modeling can be a powerful tool for: (1) clarifying the complex interdependencies among reaction, diffusion, and protein-mediated components of physiological processes; (2) formulating hypotheses and making predictions to be tested in wet-lab experiments; and (3) inferring data that are impossible to measure.
Epigenetic Biomarkers in Colorectal Cancer Patients Receiving Adjuvant or Neoadjuvant Therapy: A Systematic Review of Epidemiological Studies
Authors: Barchitta Martina , Maugeri Andrea , Li Destri Giovanni , Basile Guido , Agodi Antonella
Published: August 2019

Colorectal cancer (CRC) represents the third-most common cancer worldwide and one of the main challenges for public health. Despite great strides in the application of neoadjuvant and adjuvant therapies for rectal and colon cancer patients, each of these treatments is still associated with certain adverse effects and different response rates. Thus, there is an urgent need for identifying novel potential biomarkers that might guide personalized treatments for specific subgroups of patients. However, until now, there are no biomarkers to predict the manifestation of adverse effects and the response to treatment in CRC patients. Herein, we provide a systematic review of epidemiological studies investigating epigenetic biomarkers in CRC patients receiving neoadjuvant or adjuvant therapy, and their potential role for the prediction of outcomes and response to treatment. With this aim in mind, we identified several epigenetic markers in CRC patients who received surgery with adjuvant or neoadjuvant therapy. However, none of them currently has the robustness to be translated into the clinical setting. Thus, more efforts and further large-size prospective studies and/or trials should be encouraged to develop epigenetic biomarker panels for personalized prevention and medicine in CRC cancer.
Gene Modules Co-regulated with Biosynthetic Gene Clusters for Allelopathy between Rice and Barnyardgrass
Authors: Sultana Most. Humaira , Liu Fangjie , Alamin Md. , Mao Lingfeng , Jia Lei , Chen Hongyu , Wu Dongya , Wang Yingying , Fu Fei , Wu Sanling , Wang Weidi , Ye Chuyu , Zhu Qian-Hao , Qiu Jie , Fan Longjiang
Published: August 2019

Allelopathy is a central process in crop–weed interactions and is mediated by the release of allelochemicals that result in adverse growth effects on one or the other plant in the interaction. The genomic mechanism for the biosynthesis of many critical allelochemicals is unknown but may involve the clustering of non-homologous biosynthetic genes involved in their formation and regulatory gene modules involved in controlling the coordinated expression within these gene clusters. In this study, we used the transcriptomes from mono- or co-cultured rice and barnyardgrass to investigate the nature of the gene clusters and their regulatory gene modules involved in the allelopathic interactions of these two plants. In addition to the already known biosynthetic gene clusters in barnyardgrass we identified three potential new clusters including one for quercetin biosynthesis and potentially involved in allelopathic interaction with rice. Based on the construction of gene networks, we identified one gene regulatory module containing hub transcription factors, significantly positively co-regulated with both the momilactone A and phytocassane clusters in rice. In barnyardgrass, gene modules and hub genes co-expressed with the gene clusters responsible for 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) biosynthesis were also identified. In addition, we found three genes in barnyardgrass encoding indole-3-glycerolphosphate synthase that regulate the expression of the DIMBOA cluster. Our findings offer new insights into the regulatory mechanisms of biosynthetic gene clusters involved in allelopathic interactions between rice and barnyardgrass, and have potential implications in controlling weeds for crop protection.
Complexity of Generating Mouse Models to Study the Upper Motor Neurons: Let Us Shift Focus from Mice to Neurons
Authors: Genc Baris , Gozutok Oge , Ozdinler P. Hande
Published: August 2019

Motor neuron circuitry is one of the most elaborate circuitries in our body, which ensures voluntary and skilled movement that requires cognitive input. Therefore, both the cortex and the spinal cord are involved. The cortex has special importance for motor neuron diseases, in which initiation and modulation of voluntary movement is affected. Amyotrophic lateral sclerosis (ALS) is defined by the progressive degeneration of both the upper and lower motor neurons, whereas hereditary spastic paraplegia (HSP) and primary lateral sclerosis (PLS) are characterized mainly by the loss of upper motor neurons. In an effort to reveal the cellular and molecular basis of neuronal degeneration, numerous model systems are generated, and mouse models are no exception. However, there are many different levels of complexities that need to be considered when developing mouse models. Here, we focus our attention to the upper motor neurons, which are one of the most challenging neuron populations to study. Since mice and human differ greatly at a species level, but the cells/neurons in mice and human share many common aspects of cell biology, we offer a solution by focusing our attention to the affected neurons to reveal the complexities of diseases at a cellular level and to improve translational efforts.
The Expression Profile and Prognostic Significance of Metallothionein Genes in Colorectal Cancer
Authors: Hung Kuo-Chen , Huang Tsui-Chin , Cheng Chia-Hsiung , Cheng Ya-Wen , Lin Ding-Yen , Fan Jhen-Jia , Lee Kuen-Haur
Published: August 2019

Colorectal cancer (CRC) is a heterogeneous disease resulting from the combined influence of many genetic factors. This complexity has caused the molecular characterization of CRC to remain uncharacterized, with a lack of clear gene markers associated with CRC and the prognosis of this disease. Thus, highly sensitive tumor markers for the detection of CRC are the most essential determinants of survival. In this study, we examined the simultaneous downregulation of the mRNA levels of six metallothionein (MT) genes in CRC cell lines and public CRC datasets for the first time. In addition, we detected downregulation of these six MT mRNAs’ levels in 30 pairs of tumor (T) and adjacent non-tumor (N) CRC specimens. In order to understand the potential prognostic relevance of these six MT genes and CRC, we presented a four-gene signature to evaluate the prognosis of CRC patients. Further discovery suggested that the four-gene signature (MT1F, MT1G, MT1L, and MT1X) predicted survival better than any combination of two-, three-, four-, five-, or six-gene models. In conclusion, this study is the first to report that simultaneous downregulation of six MT mRNAs’ levels in CRC patients, and their aberrant expression together, accurately predicted CRC patients’ outcomes.
Photoactive Liposomal Formulation of PVP-Conjugated Chlorin e6 for Photodynamic Reduction of Atherosclerotic Plaque
Authors: Kałas Wojciech , Wysokińska Edyta , Przybyło Magdalena , Langner Marek , Ulatowska-Jarża Agnieszka , Biały Dariusz , Wawrzyńska Magdalena , Zioło Ewa , Gil Wojciech , Trzeciak Anna M. , Podbielska Halina , Kopaczyńska Marta
Published: August 2019

Background: Liposomes serve as delivery systems for biologically active compounds. Existing technologies inefficiently encapsulate large hydrophilic macromolecules, such as PVP-conjugated chlorin e6 (Photolon). This photoactive drug has been widely tested for therapeutic applications, including photodynamic reduction of atherosclerotic plaque. Methods: A novel formulation of Photolon was produced using “gel hydration technology”. Its pharmacokinetics was tested in Sus scrofa f. domestica. Its cellular uptake, cytotoxicity, and ability to induce a phototoxic reaction were demonstrated in J774A.1, RAW264.7 macrophages, and vascular smooth muscle (T/G HA-VSMC) as well as in vascular endothelial (HUVEC) cells. Results: Developed liposomes had an average diameter of 124.7 ± 0.6 nm (polydispersity index (PDI) = 0.055) and contained >80% of Photolon). The half-life of formulation in S. scrofa was 20 min with area under the curve (AUC) equal to 14.7. The formulation was noncytotoxic in vitro and was rapidly (10 min) and efficiently accumulated by macrophages, but not T/G HA-VSMC or HUVEC. The accumulated quantity of photosensitizer was sufficient for induction of phototoxicity in J774A.1, but not in T/G HA-VSMC. Conclusions: Due to the excellent physical and pharmacokinetic properties and selectivity for macrophages, the novel liposomal formulation of Photolon is a promising therapeutic candidate for use in arteriosclerosis treatment when targeting macrophages but not accompanying vascular tissue is critical for effective and safe therapy.
An Adaptable Device for Scalable Electrospinning of Low- and High-Viscosity Solutions
Authors: McCarty Ryan J. , Giapis Konstantinos P.
Published: August 2019

This paper summarizes the design and construction of an adaptable electrospinner capable of spinning fluids over a large range of viscosities. The design accommodates needless electrospinning technologies and enables researchers to explore a large range of testing parameters. Modular parts can be exchanged for alternative versions that adapt to the research question at hand. A rotating drum electrode immersed halfway into a solution bath provides the liquid film surface from which electrospinning occurs. We tested and assessed several electrode designs and their electrospinning performance at higher (<500 poise) viscosities. Relative humidity was found to affect the onset of electrospinning of highly viscous solutions. We demonstrate robust device performance at applied voltage up to 90 kV between the electrospinning electrode and the collector. Design and fabrication aspects are discussed in practical terms, with the intent of making this device reproducible in an academic student machine shop.
Early Airway Pathological Changes in Children: New Insights into the Natural History of Wheezing
Authors: Bonato Matteo , Tiné Mariaenrica , Bazzan Erica , Biondini Davide , Saetta Marina , Baraldo Simonetta
Published: August 2019

Asthma is a heterogeneous condition characterized by reversible airflow limitation, with different phenotypes and clinical expressions. Although it is known that asthma is influenced by age, gender, genetic background, and environmental exposure, the natural history of the disease is still incompletely understood. Our current knowledge of the factors determining the evolution from wheezing in early childhood to persistent asthma later in life originates mainly from epidemiological studies. The underlying pathophysiological mechanisms are still poorly understood. The aim of this review is to converge epidemiological and pathological evidence early in the natural history of asthma to gain insight into the mechanisms of disease and their clinical expression.
Splitting Credit Risk into Systemic, Sectorial and Idiosyncratic Components
Authors: Novales Alfonso , Chamizo Alvaro
Published: August 2019

We provide a methodology to estimate a global credit risk factor from credit default swap (CDS) spreads that can be very useful for risk management. The global risk factor (GRF) reproduces quite well the different episodes that have affected the credit market over the sample period. It is highly correlated with standard credit indices, but it contains much higher explanatory power for fluctuations in CDS spreads across sectors than the credit indices themselves. The additional information content over iTraxx seems to be related to some financial interest rates. We first use the estimated GRF to analyze the extent to which the eleven sectors we consider are systemic. After that, we use it to split the credit risk of individual firms into systemic, sectorial, and idiosyncratic components, and we perform some analyses to test that the estimated idiosyncratic components are actually firm-specific. The systemic and sectorial components explain around 65% of credit risk in the European industrial and financial sectors and 50% in the North American sectors, while 35% and 50% of risk, respectively, is of an idiosyncratic nature. Thus, there is a significant margin for portfolio diversification. We also show that our decomposition allows us to identify those firms whose credit would be harder to hedge. We end up analyzing the relationship between the estimated components of risk and some synthetic risk factors, in order to learn about the different nature of the credit risk components.
Production and Tribological Characterization of Tailored Laser-Induced Surface 3D Microtextures
Authors: Voyer Joel , Zehetner Johann , Klien Stefan , Ausserer Florian , Velkavrh Igor
Published: August 2019

The aim of the present study was firstly to determine the manufacturing feasibility of a specific surface 3D-microtexturing on steel through an ultra-short pulsed laser, and secondly to investigate the tribological properties under 2 different lubrication conditions: oil-lubricated and antifriction coated. The selected 3D-microtexture consisted of 2 different levels of quadratic micropillars having side dimensions of approximately 45 µm, heights of about 35 µm and periods of 80 µm. It was shown that the production of specific 3D-microtextures on steel substrates using an ultra-short pulsed laser was feasible, and that the reproducibility of the texture dimensions over the entire textured region was extremely good. Frictional investigations have shown that, in comparison to the benchmark (untextured samples), the 3D-microtextured samples do not induce any significant improvements in the coefficient of friction (COF) under oil-lubricated conditions, but that under antifriction coated conditions, significant improvements in the friction coefficients may be achieved. Wear-based tribological tests have shown that the antifriction coating on benchmark samples was completely depleted, which greatly influenced their friction and wear behavior, since steel-steel contact occurred during testing. For the 3D-microtexture, the antifriction coating was also partially depleted; however, it accumulated itself in the microtexture which acted as a potential lubricant reservoir.
Nanotechnology in Transportation Vehicles: An Overview of Its Applications, Environmental, Health and Safety Concerns
Authors: Shafique Muhammad , Luo Xiaowei
Published: August 2019

Nanotechnology has received increasing attention and is being applied in the transportation vehicle field. With their unique physical and chemical characteristics, nanomaterials can significantly enhance the safety and durability of transportation vehicles. This paper reviews the state-of-the-art of nanotechnology and how this technology can be applied in improving the comfort, safety, and speed of transportation vehicles. Moreover, this paper systematically examines the recent developments and applications of nanotechnology in the transportation vehicle industry, including nano-coatings, nano filters, carbon black for tires, nanoparticles for engine performance enchantment and fuel consumption reduction. Also, it introduces the main challenges for broader applications, such as environmental, health and safety concerns. Since several nanomaterials have shown tremendous performance and have been theoretically researched, they can be potential candidates for applications in future environmental friendly transportation vehicles. This paper will contribute to further sustainable research and greater potential applications of environmentally friendly nanomaterials in healthier transportation vehicles to improve the transportation industry around the globe.