bluuebunny/crossref_metadata_2025 huggingface dataset:
Deviation Tolerance Performance Evaluation and Experiment of Picking End Effector for Famous Tea
Authors: Zhu Yingpeng , Wu Chuanyu , Tong Junhua , Chen Jianneng , He Leiying , Wang Rongyang , Jia Jiangming
Published: February 2021

Accurately obtaining the posture and spatial position of tea buds through machine vision and other technologies is difficult due to the small size, different shapes, and complex growth environment of tea buds. Therefore, end effectors are prone to problems, such as picking omission and picking error. This study designs a picking end effector based on negative pressure guidance for famous tea. This end effector uses negative pressure to guide tea buds in a top-down manner, thereby correcting their posture and spatial position. Therefore, the designed end effector has deviation tolerance performance that can improve the picking success rate. The pre-experiment is designed, the tip of apical bud is referred to as the descent position, and the negative pressure range is determined to be 0.6 to 0.9 kPa. A deviation tolerance orthogonal experiment is designed. Experimental results show that various experimental factors are ranked in terms of the significance level of the effect on the average success rate, and the significance ranking is as follows: negative pressure (P) > pipe diameter (D) > descent speed (V). An evaluation method of deviation tolerance performance is presented, and the optimal experiment factor-level combination is determined as: P = 0.9 kPa, D = 34 mm, V = 20 mm/s. Within the deviation range of a 10 mm radius, the average success rate of the negative pressure guidance of the end effector is 97.36%. The designed end effector can be applied to the intelligent picking of famous tea. This study can provide a reference for the design of similar picking end effectors for famous tea.
Correlation Analysis of High-Throughput Fruit Phenomics and Biochemical Profiles in Native Peppers (Capsicum spp.) from the Primary Center of Diversification
Authors: González-López Jorge , Rodríguez-Moar Simón , Silvar Cristina
Published: January 2021

The main goal of this work was to investigate the relationship between the fruit morphology and biochemical composition of peppers (Capsicum spp.). For that purpose, one hundred native varieties from the Andean region, where the genus Capsicum has its origin, were analysed for different phytochemical compounds. In addition, pepper fruits were assessed with the highly precise phenomics tool Tomato Analyzer. The collection showed a broad variability which was more evident within the C. annuum group. On average, C. frutescens accessions displayed the highest levels of solid soluble content, pH, polyphenols and antioxidant activity. The Tomato Analyzer descriptors under the categories of size, shape index, and latitudinal section, mostly contributed to the variance among Capsicum groups. C. annuum hold the larger fruits, whereas C. frutescens comprised fruits of smaller sizes. The correlation analysis revealed that biochemical traits were negatively correlated with the fruit parameters related to size, suggesting that huger fruits contain lower amounts of chemical metabolites. The multivariate approximations demonstrated that Andean peppers assorted according to morphometric and colorimetric characteristics, but independently of their species or geographical origin. Groups of valuable native varieties carrying promising traits were identified.
High-Oleic Palm Oil (HOPO) Production from Parthenocarpic Fruits in Oil Palm Interspecific Hybrids Using Naphthalene Acetic Acid
Authors: Romero Hernán Mauricio , Daza Edison , Ayala-Díaz Iván , Ruiz-Romero Rodrigo
Published: February 2021

Interspecific OxG hybrids of African palm Elaeis guineensis Jacq. and the American palm Elaeis oleifera Cortes produce high-oleic palm oil (HOPO) with low saturated fatty acid content. OxG hybrids are highly productive, grow slowly, and are resistant to bud rot disease. However, OxG hybrid pollen presents low viability and germinability, so assisted pollination is a must. Hybrids can produce parthenocarpic or seedless fruits, with the exogenous application of plant growth regulators. Thus, naphthalene acetic acid (NAA) effects on parthenocarpic fruits induction, bunch formation, and oil quality were evaluated. The OxG hybrid Coari x La Mé was used. NAA doses, frequency, number of applications, and the phenological stages for the treatments were defined. A total dose of 1200 mg L−1 NAA applied three or four times produced bunches with better fruit set, similar average bunch weight, and oil to dry mesocarp than those obtained with assisted pollination. At a semi-commercial scale, 1200 mg L−1 NAA induced bunches that consisted of 93% or more of seedless fruits. Bunch number (2208 ± 84 versus 1690 ± 129) and oil to bunch (32.2 ± 0.7 versus 25.3 ± 0.8) were higher in the NAA induced bunches than in the assisted pollination. However, the average bunch weight was lower (12.2 ± 0.4 versus 14.9 ± 0.6). NAA increased oil to bunch in 36% (8.7 ± 0.1 versus 6.4 ± 0.3). Thus, with this technology, it is plausible to reach more than 10 tons per hectare per year of HOPO. Potentially, without increasing the planted oil palm area, OxG hybrids and NAA applications could alone meet the world’s fats and oil demands.
Enhanced Carbon Sequestration in Marginal Land Upon Shift towards Perennial C4Miscanthus × giganteus: A Case Study in North-Western Czechia
Authors: Al Souki Karim Suhail , Burdová Hana , Trubač Jakub , Štojdl Jiří , Kuráň Pavel , Kříženecká Sylvie , Machová Iva , Kubát Karel , Popelka Jan , Malinská Hana Auer , Nebeská Diana , Ust’ak Sergej , Honzík Roman , Trögl Josef
Published: February 2021

Bioenergy crops such as Miscanthus × giganteus are foreseeable as an alternative source to replace fossil fuel and reduce greenhouse gas emissions. They are also assessed as an environment-friendly solution for polluted, marginal and low-quality agricultural soils. Several studies had been launched on soil organic carbon sequestration potentials of miscanthus culture along with its impacts on restoring soil functionality, most of which focus on the long-term basis of the plant’s cultivation. Nevertheless, information concerning the short term impacts as well as the situation in Czechia is still scarce. In this context, a field experiment was launched in 2017 in a poor-quality agricultural land in the city of Chomutov (North-Western Czechia) to compare the impacts of the perennial C4 miscanthus with an annual C3 forage crop (wheat) on the soil carbon stocks as well as enhancing its functionality. Results through the 0–30 cm soil profile examination showed that miscanthus plants played a role in improving the studied soil physico-chemical (bulk density and soil organic carbon concentrations) and biological (Phospholipid fatty acids stress indicator, basal respiration and fluorescein diacetate hydrolytic activity) parameters. The naturally occurring δ13C concentrations were used to evaluate the direct plant contribution to the total soil organic carbon (SOC) stocks and revealed considerable miscanthus contribution all over the detected soil layers (1.98 ± 0.21 Mg C. ha−1 yr−1) after only 3 growing seasons. It is thus suggested that the C4 perennial miscanthus possess remarkable prospects for SOC sequestration and restoring degraded lands.
Characteristics of Cowsheds in Vietnamese Smallholder Dairy Farms and Their Associations with Microclimate—A Preliminary Study
Authors: Bang Nguyen N. , Gaughan John B. , Hayes Ben J. , Lyons Russell E. , Chanh Nguyen V. , Trach Nguyen X. , Khang Duong N. , McNeill David M.
Published: January 2021

In smallholder dairy farms (SDFs), farmers often build cowsheds using local materials and based on self-accumulated experience without due consideration to reducing the risk of heat stress. This study aimed to characterise the heat stress abatement strategies and microclimate within SDF cowsheds from four typical dairy regions of Vietnam (south lowland, south highland, north lowland and north highland) and identify the housing parameters most associated with the microclimate. The study was conducted on 32 SDFs (eight SDFs per region) in autumn 2017. Twelve housing management variables, illustrating cowshed design and heat stress abatement methods of each SDF, were collected. Six microclimate parameters, collected within the cowshed, were temperature (AT), humidity, air speed (AS), heat load index (HLI), Temperature-humidity index (THI) and accumulated heat load units (AHLU) during a day (06:00 h to 18:00 h). Factor analysis and cluster analysis was applied to group cowsheds of SDFs into clusters where SDFs in the cluster had the same cowshed characteristics. Multivariable linear models were applied to define the parameters most likely to inform future research into heat stress mitigation on SDF. Averaged from 08:00 h to 18:00 h, microclimate inside the cowsheds was considered hot (HLI > 79) in the highland and very hot (HLI > 86) in the lowland regions. Cows in the lowland regions accumulated high heat load (AHLU > 50) by 18:00 h. Cowsheds of SDFs varied widely and grouped into seven cowshed types, but no type was more effective than others in reducing heat stress conditions within cowsheds. Using roof soakers together with fans decreased AT and HLI by 1.3 °C and 3.2 units, respectively, at 14:00 h compared to 11:00 h. Each 100 m increase in altitude was associated with decreases of 0.4 °C in AT, 1.3 units in HLI and 0.8 units in THI (p < 0.001). Each meter increase in the eave height of the cowshed roof was associated with decreases of 0.87 °C in AT, 3.31 units in HLI and 1.42 units in THI, and an increase of 0.14 m/s in AS (p < 0.05). The cowshed parameters that should be prioritised for future research into the amelioration of heat stress in SDF cows include using the roof soakers together with fans, increasing altitude, eave roof height and floor area per cow.
Pedigree and Molecular Analyses in the Assessment of Genetic Variability of the Polish Greyhound
Authors: Goleman Małgorzata , Balicki Ireneusz , Radko Anna , Rozempolska-Rucińska Iwona , Zięba Grzegorz
Published: January 2021

The aim of the study was to assess the genetic variability of the Polish Greyhound population based on pedigree analysis and molecular DNA testing and to determine the degree of relatedness among individuals in the population. Pedigree data of 912 Polish Greyhounds recorded in pedigree books since they were opened for this breed were analyzed. For molecular testing, DNA was obtained from cheek swabs taken from 235 dogs of the tested breed. A panel of 21 markers (Short Tandem Repeat—STR) was used. The mean inbreeding determined for the Polish Greyhound population based on pedigree analyses was low and amounted to 11.8%, but as many as 872 individuals of the 912 dogs in the studied population were inbred. A total of 83 founders (at least one unknown parent) were identified, among which 27 founders had both unknown parents. Full-sibling groups consisted of 130 individuals, with a minimum and maximum litter size of 2 and 16, respectively. The average litter size was 5.969. Gene diversity calculated based on the mean kinship matrix was 0.862 and the population mean kinship was 0.138. The founder genome equivalent based on the mean kinship matrix was 3.61; the founder genome surviving level was 12.34; the mean Ne was estimated at 21.76; and the Ne/N ratio was 0.135. The FIS inbreeding coefficient for 21 STR was negative, and the mean FIS value for all loci had a low negative value (−0.018). These values suggest a low level of inbreeding in the examined breed as well as the avoidance of mating related animals.
Bioactive Coatings Based on Hydroxyapatite, Kanamycin, and Growth Factor for Biofilm Modulation
Authors: Gherasim Oana , Grumezescu Alexandru Mihai , Grumezescu Valentina , Negut Irina , Dumitrescu Marius Florin , Stan Miruna Silvia , Nica Ionela Cristina , Holban Alina Maria , Socol Gabriel , Andronescu Ecaterina
Published: February 2021

The occurrence of opportunistic local infections and improper integration of metallic implants results in severe health conditions. Protective and tunable coatings represent an attractive and challenging selection for improving the metallic devices’ biofunctional performances to restore or replace bone tissue. Composite materials based on hydroxyapatite (HAp), Kanamycin (KAN), and fibroblast growth factor 2 (FGF2) are herein proposed as multifunctional coatings for hard tissue implants. The superior cytocompatibility of the obtained composite coatings was evidenced by performing proliferation and morphological assays on osteoblast cell cultures. The addition of FGF2 proved beneficial concerning the metabolic activity, adhesion, and spreading of cells. The KAN-embedded coatings exhibited significant inhibitory effects against bacterial biofilm development for at least two days, the results being superior in the case of Gram-positive pathogens. HAp-based coatings embedded with KAN and FGF2 protein are proposed as multifunctional materials with superior osseointegration potential and the ability to reduce device-associated infections.
Protective Effects of Kirenol against Lipopolysaccharide-Induced Acute Lung Injury through the Modulation of the Proinflammatory NFκB Pathway and the AMPK2-/Nrf2-Mediated HO-1/AOE Pathway
Authors: Lin Frank Cheau-Feng , Lee Shiuan-Shinn , Li Yi-Ching , Ho Yung-Chuan , Chen Wen-Ying , Chen Chun-Jung , Lee Min-Wei , Yeh Kun-Lin , Tsai Stella Chin-Shaw , Kuan Yu-Hsiang
Published: January 2021

Acute lung injury (ALI) is an acute and life-threatening inflammatory disease of the lung parenchyma that is associated with high mortality worldwide. No therapeutic strategies have been developed for the mitigation of the proinflammatory response that characterizes ALI. Kirenol has anti-inflammatory, antiarthritic, and immunoregulatory effects. In the present study, we investigated the protective effects of kirenol against lipopolysaccharides (LPS)-induced ALI in mice. Kirenol reduced the LPS-induced histopathology changes involving edema and thickening of the interstitial or alveolar walls, infiltration of leukocytes, formation of hyaline membrane. Pretreatment with kirenol reduced leukocytes infiltration in bronchoalveolar lavage fluid (BALF), the alveolar-capillary barrier disruption and lipid peroxidation in lung tissues induced by LPS. Kirenol significantly inhibited the secretion of cytokines, IL-1β, IL6, and TNFα, into the BALF of the mice with LPS-induced ALI through NFκB activation. Moreover, kirenol attenuated the downregulation of the antioxidant enzymes, superoxide dismutase, glutathione peroxidase, and catalase that was induced by LPS. HO-1 expression and the phosphorylation of Nrf2 and AMPK2 were also induced by kirenol. The results indicate that kirenol can be developed as a treatment strategy for ALI, and its effects are induced through the inhibition of the NF-κB proinflammatory pathway and promotion of AMPK2/Nrf2-mediated HO-1 and antioxidant enzymes (AOE) activation.
Blunted Reducing Power Generation in Erythrocytes Contributes to Oxidative Stress in Prepubertal Obese Children with Insulin Resistance
Authors: González-Domínguez Álvaro , Visiedo Francisco , Domínguez-Riscart Jesus , Ruiz-Mateos Beatriz , Saez-Benito Ana , Lechuga-Sancho Alfonso M. , Mateos Rosa María
Published: February 2021

Childhood obesity, and specifically its metabolic complications, are related to deficient antioxidant capacity and oxidative stress. Erythrocytes are constantly exposed to multiple sources of oxidative stress; hence, they are equipped with powerful antioxidant mechanisms requiring permanent reducing power generation and turnover. Glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) are two key enzymes on the pentose phosphate pathway. Both enzymes supply reducing power by generating NADPH, which is essential for maintaining the redox balance within the cell and the activity of other antioxidant enzymes. We hypothesized that obese children with insulin resistance would exhibit blunted G6PDH and 6PGDH activities, contributing to their erythrocytes’ redox status imbalances. We studied 15 control and 24 obese prepubertal children, 12 of whom were insulin-resistant according to an oral glucose tolerance test (OGTT). We analyzed erythroid malondialdehyde (MDA) and carbonyl group levels as oxidative stress markers. NADP+/NADPH and GSH/GSSG were measured to determine redox status, and NADPH production by both G6PDH and 6PGDH was assayed spectrophotometrically to characterize pentose phosphate pathway activity. Finally, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) activities were also assessed. As expected, MDA and carbonyl groups levels were higher at baseline and along the OGTT in insulin-resistant children. Both redox indicators showed an imbalance in favor of the oxidized forms along the OGTT in the insulin-resistant obese group. Additionally, the NADPH synthesis, as well as GR activity, were decreased. H2O2 removing enzyme activities were depleted at baseline in both obese groups, although after sugar intake only metabolically healthy obese participants were able to maintain their catalase activity. No change was detected in SOD activity between groups. Our results show that obese children with insulin resistance present higher levels of oxidative damage, blunted capacity to generate reducing power, and hampered function of key NADPH-dependent antioxidant enzymes.
Minimum Relevant Features to Obtain Explainable Systems for Predicting Cardiovascular Disease Using the Statlog Data Set
Authors: Porto Roberto , Molina José M. , Berlanga Antonio , Patricio Miguel A.
Published: January 2021

Learning systems have been focused on creating models capable of obtaining the best results in error metrics. Recently, the focus has shifted to improvement in the interpretation and explanation of the results. The need for interpretation is greater when these models are used to support decision making. In some areas, this becomes an indispensable requirement, such as in medicine. The goal of this study was to define a simple process to construct a system that could be easily interpreted based on two principles: (1) reduction of attributes without degrading the performance of the prediction systems and (2) selecting a technique to interpret the final prediction system. To describe this process, we selected a problem, predicting cardiovascular disease, by analyzing the well-known Statlog (Heart) data set from the University of California’s Automated Learning Repository. We analyzed the cost of making predictions easier to interpret by reducing the number of features that explain the classification of health status versus the cost in accuracy. We performed an analysis on a large set of classification techniques and performance metrics, demonstrating that it is possible to construct explainable and reliable models that provide high quality predictive performance.
Facial Expression Recognition Based on Multi-Features Cooperative Deep Convolutional Network
Authors: Wu Haopeng , Lu Zhiying , Zhang Jianfeng , Li Xin , Zhao Mingyue , Ding Xudong
Published: February 2021

This paper addresses the problem of Facial Expression Recognition (FER), focusing on unobvious facial movements. Traditional methods often cause overfitting problems or incomplete information due to insufficient data and manual selection of features. Instead, our proposed network, which is called the Multi-features Cooperative Deep Convolutional Network (MC-DCN), maintains focus on the overall feature of the face and the trend of key parts. The processing of video data is the first stage. The method of ensemble of regression trees (ERT) is used to obtain the overall contour of the face. Then, the attention model is used to pick up the parts of face that are more susceptible to expressions. Under the combined effect of these two methods, the image which can be called a local feature map is obtained. After that, the video data are sent to MC-DCN, containing parallel sub-networks. While the overall spatiotemporal characteristics of facial expressions are obtained through the sequence of images, the selection of keys parts can better learn the changes in facial expressions brought about by subtle facial movements. By combining local features and global features, the proposed method can acquire more information, leading to better performance. The experimental results show that MC-DCN can achieve recognition rates of 95%, 78.6% and 78.3% on the three datasets SAVEE, MMI, and edited GEMEP, respectively.
Blood Wettability of Different Dental Implant Surfaces after Different Pre-Treatments: Ultrasonic Instrumentation, Platelet-Rich Fibrin Coating, and Acid Etching. An In Vitro Study
Authors: Bevilacqua Lorenzo , Faccioni Francesco , Porrelli Davide , Faccioni Paolo , Rusin Francesco , Frassetto Andrea , Maglione Michele
Published: February 2021

Background: The blood wettability of titanium implant surfaces favors primary osseointegration and tissue healing in peri-implantitis. The current study aims to evaluate this property on the main different kinds of surfaces, brand new and after treatments. Methods: grade IV titanium disks were used, five machined, five laser-treated, five sandblasted. The experiment was on four steps. Between each step, specimens were sterilized. At the end of each step, a 4 µL blood drop was put on the samples, and contact angles were calculated. The steps consisted of—1: no treatment; 2: surface instrumentation with an ultrasonic titanium tip; 3: platelet-rich fibrin (PRF) coating and drying with sterile gauze; 4: etching with phosphoric acid, rinse and saline solution and air-drying. At the end of each phase, a blood drop was placed on the surfaces. Blood for PRF preparation and for calculation of contact angles was collected from a single source. Results: average wettability decreased after instrumentation, and increased after the PRF application to a superior level both to the first and second steps. The highest wettability was obtained after etching. This trend is statistically significant for machined and sandblasted surfaces. Conclusions: In the clinical environment, PRF and phosphoric acid used for conditioning exposed implant surfaces can be used for the healing of peri-implant tissues.
Optimization of Brake Calipers Using Topology Optimization for Additive Manufacturing
Authors: Tyflopoulos Evangelos , Lien Mathias , Steinert Martin
Published: February 2021

The weight optimization of a structure can be conducted by using fewer and downsized components, applying lighter materials in production, and removing unwanted material. Topology optimization (TO) is one of the most implemented material removal processes. In addition, when it is oriented towards additive manufacturing (AM), it increases design flexibility. The traditional optimization approach is the compliance optimization, where the material layout of a structure is optimized by minimizing its overall compliance. However, TO, in its current state of the art, is mainly used for design inspiration and not for manufacturing due to design complexities and lack of accuracy of its design solutions. The authors, in this research paper, explore the benefits and the limitations of the TO using as a case study the housings of a front and a rear brake caliper. The calipers were optimized for weight reduction by implementing the aforementioned optimization procedure. Their housings were topologically optimized, partially redesigned, prepared for 3D printing, validated, and 3D printed in titanium using selective laser melting (SLM). The weight of the optimized calipers reduced by 41.6% compared to commercial calipers. Designers interested in either TO or in automotive engineering can exploit the findings in this paper.
Testing as-Built Quality of Free-Form Panels: Lessons Learned from a Case Study and Mock-up Panel Tests
Authors: Ock Jong-Ho
Published: February 2021

Constructing free-form buildings is very complex due to the difficulty in fabricating the curved façade. To install the façade, the complex geometric shapes of the façade need to be divided into panels. The panels developed are classified into three categories in terms of their curvatures, i.e., planar, single-curved, double-curved panels. The quality of the curved façade is determined by the geometric difference between as-built and as-designed panel shapes. Among the three types of curved panels, the double-curved panel is very difficult to form, showing greater quality discrepancy than the other two panel types. Ensuring the as-built quality of the curved façade is for contractors. The main objective of this study is to enhance small/mid-size contractors’ capacity of managing the as-built quality of the double-curved panel. To meet the study objectives, a case study of a small free-form building and empirical mock-up tests of curved panels were performed and beneficial lessons for the contractors were identified through the tests. Among diverse materials, aluminum and glass-fiber-reinforced concrete (GFRC) were utilized for the mock-up tests. Three-dimensional laser scanning technology was employed to foster the as-built data of the case study project and the mocked-up double-curved panels. The data superimposition method was used to measure the deviation between the as-designed and the as-built data of the case study.
Emergency Repair Scheduling Model for Road Network Integrating Rescheduling Feature
Authors: Liu Shu-Shun , Arifin Muhammad , Chen Wei , Huang Ying-Hua
Published: February 2021

When a natural disaster occurs, road maintenance departments always face the challenge of how to assign repair resources properly to recover damaged road segments as soon as possible. From the literature review, most studies treat such problems as a vehicle routing problem (VRP). In those studies, repair resources are always dispatched as complete crews, and cannot be divided into smaller scales. Furthermore, each disaster point is only allowed one group of resources to recover it, without considering the possibility of accelerating the production rate subjected to specific objectives. Such limitation restricts required resources in an inflexible manner. Therefore, this study defines all repair works as an emergency repair project and adopts the framework of the Resource-Constrained Project Scheduling Problem (RCPSP), which can resolve such complicated resource assignment issue. A novel emergency repair scheduling model for the road network is proposed based on Constraint Programming (CP) as the searching algorithm to facilitate model formulation. According to the RCPSP concepts, disaster points are set as repair activities and resource travel routes between disaster points are set as transit activities. All the repair activities are linked by transit activities and the required resources are assigned accordingly. In order to consider the second-wave hazard events of where new disaster points may occur, and new resources may be added into emergency repair projects, a rescheduling feature is integrated into the proposed model. Through two case studies, research findings show that this model can be easily modulated to adapt to different situations satisfying practical disaster management goals and solving emergency repair scheduling problems for road networks efficiently.
A Serious Gaming Approach for Crowdsensing in Urban Water Infrastructure with Blockchain Support
Authors: Predescu Alexandru , Arsene Diana , Pahonțu Bogdan , Mocanu Mariana , Chiru Costin
Published: February 2021

This paper presents the current state of the gaming industry, which provides an important background for an effective serious game implementation in mobile crowdsensing. An overview of existing solutions, scientific studies and market research highlights the current trends and the potential applications for citizen-centric platforms in the context of Cyber–Physical–Social systems. The proposed solution focuses on serious games applied in urban water management from the perspective of mobile crowdsensing, with a reward-driven mechanism defined for the crowdsensing tasks. The serious game is designed to provide entertainment value by means of gamified interaction with the environment, while the crowdsensing component involves a set of roles for finding, solving and validating water-related issues. The mathematical model of distance-constrained multi-depot vehicle routing problem with heterogeneous fleet capacity is evaluated in the context of the proposed scenario, with random initial conditions given by the location of players, while the Vickrey–Clarke–Groves auction model provides an alternative to the centralized task allocation strategy, subject to the same evaluation method. A blockchain component based on the Hyperledger Fabric architecture provides the level of trust required for achieving overall platform utility for different stakeholders in mobile crowdsensing.
A Review of Forward-Dynamics Simulation Models for Predicting Optimal Technique in Maximal Effort Sporting Movements
Authors: McErlain-Naylor Stuart A. , King Mark A. , Felton Paul J.
Published: February 2021

The identification of optimum technique for maximal effort sporting tasks is one of the greatest challenges within sports biomechanics. A theoretical approach using forward-dynamics simulation allows individual parameters to be systematically perturbed independently of potentially confounding variables. Each study typically follows a four-stage process of model construction, parameter determination, model evaluation, and model optimization. This review critically evaluates forward-dynamics simulation models of maximal effort sporting movements using a dynamical systems theory framework. Organismic, environmental, and task constraints applied within such models are critically evaluated, and recommendations are made regarding future directions and best practices. The incorporation of self-organizational processes representing movement variability and “intrinsic dynamics” remains limited. In the future, forward-dynamics simulation models predicting individual-specific optimal techniques of sporting movements may be used as indicative rather than prescriptive tools within a coaching framework to aid applied practice and understanding, although researchers and practitioners should continue to consider concerns resulting from dynamical systems theory regarding the complexity of models and particularly regarding self-organization processes.
The Opto-Electronic Functional Devices Based on Three-Dimensional Lead Halide Perovskites
Authors: Liu Henan , Zhang Hao , Xu Xunling , Zhang Lin
Published: February 2021

These days, opto-electronic functional devices based on three-dimensional lead halide perovskites (LHPs) are emerging. LHPs could be spin-coated to other materials, making it very convenient to combine LHPs with different categories of materials including metals, semiconductors, and polymers and achieve high-level performances. In this review, we will discuss the development in the LHP-based functional devices in recent years. After a brief presentation of the LHP’s properties, we will focus on the functional devices including lasers, photodetectors, and modulators. Then the fabrication of the LHP-based devices will be presented, which is followed by the summary and outlook.
Promising Immobilization of Industrial-Class Phospholipase A1 to Attain High-Yield Phospholipids Hydrolysis and Repeated Use with Optimal Water Content in Water-in-Oil Microemulsion Phase
Authors: Hayakawa Yusuke , Nakayama Ryoichi , Namiki Norikazu , Imai Masanao
Published: February 2021

In this study, we maximized the reactivity of phospholipids hydrolysis with immobilized industrial-class phospholipase A1 (PLA1) at the desired water content in the water-in-oil (W/O) microemulsion phase. The optimal hydrophobic-hydrophilic condition of the reaction media in a hydrophobic enzyme reaction is critical to realize the maximum yields of enzyme activity of phospholipase A1. It was attributed to enzymes disliking hydrophobic surroundings as a special molecular structure for reactivity. Immobilization of PLA1 was successfully achieved with the aid of a hydrophobic carrier (Accurel MP100) combination with the treatment using glutaraldehyde. The immobilized yield was over 90% based on simple adsorption. The hydrolysis reaction was kinetically investigated through the effect of glutaraldehyde treatment of carrier and water content in the W/O microemulsion phase. The initial reaction rate increased linearly with an increasing glutaraldehyde concentration and then leveled off over a 6% glutaraldehyde concentration. The initial reaction rate, which was predominantly driven by the water content in the organic phase, changed according to a typical bell-shaped curve with respect to the molar ratio of water to phospholipid. It behaved in a similar way with different glutaraldehyde concentrations. After 10 cycles of repeated use, the reactivity was well sustained at 40% of the initial reaction rate and the creation of the final product. Accumulated yield after 10 times repetition was sufficient for industrial applications. Immobilized PLA1 has demonstrated potential as a biocatalyst for the production of phospholipid biochemicals.
Multi-Compartment Lymph-Node-on-a-Chip Enables Measurement of Immune Cell Motility in Response to Drugs
Authors: Hallfors Nicholas , Shanti Aya , Sapudom Jiranuwat , Teo Jeremy , Petroianu Georg , Lee SungMun , Planelles Lourdes , Stefanini Cesare
Published: January 2021

Organs On-a-Chip represent novel platforms for modelling human physiology and disease. The lymph node (LN) is a relevant immune organ in which B and T lymphocytes are spatially organized in a complex architecture, and it is the place where the immune response initiates. The present study addresses the utility of a recently designed LN-on-a-chip to dissect and understand the effect of drugs delivered to cells in a fluidic multicellular 3D setting that mimics the human LN. To do so, we analyzed the motility and viability of human B and T cells exposed to hydroxychloroquine (HCQ). We show that the innovative LN platform, which operates at a microscale level, allows real-time monitoring of co-cultured B and T cells by imaging, and supports cellular random movement. HCQ delivered to cells through a constant and continuous flow induces a reduction in T cell velocity while promotes persistent rotational motion. We also find that HCQ increases the production of reactive oxygen species in T cells. Taken together, these results highlight the potential of the LN-on-a-chip to be applied in drug screening and development, and in cellular dynamics studies.
Protective Role of Galanin during Chemically Induced Inflammation in Zebrafish Larvae
Authors: Nowik Natalia , Przyborowska Anna , Sienkiewicz Waldemar , Podlasz Piotr
Published: January 2021

During a pathological condition, many different systems are involved in the response of an affected organism. Galanin is considered to be a neuropeptide that plays an important role in the central nervous system; however, it is involved in many other biological processes, including the immune response. During our studies, we showed that galanin became upregulated in zebrafish larvae when exposed to copper sulfate. Moreover, the presence of normal levels of galanin, administration of a galanin analog NAX 5055 or galanin overexpression led to lowered lateral line damage and enhanced expression of inflammatory markers compared to the knockout larvae. The results showed that the neuroendocrine system acts multifunctionally and should be considered as a part of the complex neuro–immune–endocrine axis.
The Dynamics of the Bacterial Community of the Photobioreactor-Cultivated Green Microalga Haematococcus lacustris during Stress-Induced Astaxanthin Accumulation
Authors: Chekanov Konstantin , Zaytseva Anna , Mamedov Ilgar , Solovchenko Alexei , Lobakova Elena
Published: February 2021

Haematococcus lacustris is a natural source of a valuable ketocarotenoid astaxanthin. Under autotrophic growth conditions, it exists in the form of a community with bacteria. The close coexistence of these microorganisms raises two questions: how broad their diversity is and how they interact with the microalga. Despite the importance these issues, little is known about microorganisms existing in Haematococcus cultures. For the first time, we characterize the dynamic of the H. lacustris microbiome of the microbiome of Haematococcus (a changeover of the bacterial associated species as function of the time) cultivated autotrophically in a photobioreactor based on 16S rRNA metabarcoding data. We found that Proteobacteria and Bacteroidetes are predominant phyla in the community. The Caulobacter bacterium became abundant during astaxanthin accumulation. These data were supported by microscopy. We discuss possible roles and interactions of the community members. These findings are of potential significance for biotechnology. They provide an insight into possible bacterial contamination in algal biomass and reveal the presence of bacteria essential for the algal growth.
Osteoclasts Differentiation from Murine RAW 264.7 Cells Stimulated by RANKL: Timing and Behavior
Authors: Lampiasi Nadia , Russo Roberta , Kireev Igor , Strelkova Olga , Zhironkina Oxana , Zito Francesca
Published: February 2021

The development of multi-nucleated cells is critical for osteoclasts (OCs) maturation and function. Our objective was to extend knowledge on osteoclastogenesis, focusing on pre-OC fusion timing and behavior. RAW 264.7 cells, which is a murine monocyte-macrophage cell line, provide a valuable and widely used tool for in vitro studies on osteoclastogenesis mechanisms. Cells were treated with the receptor activator of nuclear factor κ-B ligand (RANKL) for 1–4 days and effects on cell morphology, cytoskeletal organization, protein distribution, and OC-specific gene expression examined by TEM, immunofluorescence, and qPCR. Multinucleated cells began to appear at two days of Receptor Activator of Nuclear factor κ-B Ligand (RANKL) stimulation, increasing in number and size in the following days, associated with morphological and cytoskeletal organization changes. Interesting cellular extensions were observed in three days within cells labeled with wheat germ agglutinin (WGA)-Fluorescein isothiocyanate (FITC). The membrane, cytoplasmic, or nuclear distribution of RANK, TRAF6, p-p38, pERK1/2, and NFATc1, respectively, was related to OCs maturation timing. The gene expression for transcription factors regulating osteoclastogenesis (NFATc1, c-fos, RelA, MITF), molecules involved in RANKL-signaling transduction (TRAF6), cytoskeleton regulation (RhoA), fusion (DC-STAMP), migration (MMP9), and OC-specific enzymes (TRAP, CtsK), showed different trends related to OC differentiation timing. Our findings provide an integrated view on the morphological and molecular changes occurring during RANKL stimulation of RAW 264.7 cells, which are important to better understand the OCs’ maturation processes.
Comparison of the Acute Effects of Hold-Relax and Static Stretching among Older Adults
Authors: Nakamura Masatoshi , Sato Shigeru , Kiyono Ryosuke , Yahata Kaoru , Yoshida Riku , Fukaya Taizan , Konrad Andreas
Published: February 2021

Various stretching techniques are generally recommended to counteract age-related declines in range of motion (ROM) and/or increased muscle stiffness. However, to date, an effective stretching technique has not yet been established for older adults. Consequently, we compared the acute effects of hold relax stretching (HRS) and static stretching (SS) on dorsiflexion (DF) ROM and muscle stiffness among older adults. Overall, 15 elderly men and nine elderly women (70.2 ± 3.9 years, 160.8 ± 7.8 cm, 59.6 ± 9.7 kg) were enrolled, and both legs were randomized to either HRS or SS stretching. We measured DF ROM and muscle stiffness using a dynamometer and ultrasonography before and after 120 s of HRS or SS interventions. Our multivariate analysis indicated no significant interaction effects, but a main effect for DF ROM. Post-hoc tests revealed that DF ROM was increased after both HRS and SS interventions. Moreover, multivariate analysis showed a significant interaction effect for muscle stiffness. Post-hoc tests revealed that muscle stiffness was decreased significantly after only SS intervention. Taken together, our results indicated that both HRS and SS interventions are recommended to increase ROM, and SS is recommended to decrease muscle stiffness in older adults.
Different Aggregation Pathways and Structures for Aβ40 and Aβ42 Peptides
Authors: Wang Li , Eom Kilho , Kwon Taeyun
Published: January 2021

Self-aggregation of amyloid-β (Aβ) peptides has been known to play a vital role in the onset stage of neurodegenerative diseases, indicating the necessity of understanding the aggregation process of Aβ peptides. Despite previous studies on the aggregation process of Aβ peptides, the aggregation pathways of Aβ isoforms (i.e., Aβ40 and Aβ42) and their related structures have not been fully understood yet. Here, we study the aggregation pathways of Aβ40 and Aβ42, and the structures of Aβ40 and Aβ42 aggregates during the process, based on fluorescence and atomic force microscopy (AFM) experiments. It is shown that in the beginning of aggregation process for both Aβ40 and Aβ42, a number of particles (i.e., spherical oligomers) are formed. These particles are subsequently self-assembled together, resulting in the formation of different shapes of amyloid fibrils. Our finding suggests that the different aggregation pathways of Aβ isoforms lead to the amyloid fibrils with contrasting structure.
ER Stress and Unfolded Protein Response in Leukemia: Friend, Foe, or Both?
Authors: Féral Kelly , Jaud Manon , Philippe Céline , Di Bella Doriana , Pyronnet Stéphane , Rouault-Pierre Kevin , Mazzolini Laurent , Touriol Christian
Published: January 2021

The unfolded protein response (UPR) is an evolutionarily conserved adaptive signaling pathway triggered by a stress of the endoplasmic reticulum (ER) lumen compartment, which is initiated by the accumulation of unfolded proteins. This response, mediated by three sensors-Inositol Requiring Enzyme 1 (IRE1), Activating Transcription Factor 6 (ATF6), and Protein Kinase RNA-Like Endoplasmic Reticulum Kinase (PERK)—allows restoring protein homeostasis and maintaining cell survival. UPR represents a major cytoprotective signaling network for cancer cells, which frequently experience disturbed proteostasis owing to their rapid proliferation in an usually unfavorable microenvironment. Increased basal UPR also participates in the resistance of tumor cells against chemotherapy. UPR activation also occurs during hematopoiesis, and growing evidence supports the critical cytoprotective role played by ER stress in the emergence and proliferation of leukemic cells. In case of severe or prolonged stress, pro-survival UPR may however evolve into a cell death program called terminal UPR. Interestingly, a large number of studies have revealed that the induction of proapoptotic UPR can also strongly contribute to the sensitization of leukemic cells to chemotherapy. Here, we review the current knowledge on the consequences of the deregulation of UPR signaling in leukemias and their implications for the treatment of these diseases.
Evaluation of Metaheuristic-Based Methods for Optimization of Truss Structures via Various Algorithms and Lèvy Flight Modification
Authors: Bekdaş Gebrail , Yucel Melda , Nigdeli Sinan Melih
Published: January 2021

Truss structures are one of the major civil engineering members studied in the optimization research area. In this area, various optimization applications such as topology, size, cost, weight, material usage, etc., can be conducted for different truss structure types. In this scope with the present study, various optimization processes were carried out concerning two different large-scale space trusses to minimize the structural weight. According to this state, three structural models provided via two different truss structures, including 25 bar and 72 bar truss models, were handled for evaluation of six different metaheuristics together with the modification of Lèvy flight for three of the algorithms using swarm intelligence by considering both constant and variable populations, and different ranges for iterations, too. Additionally, the effects of the Lèvy flight function and whether it is successful or not in terms of the target of optimization were also investigated by comparing with some documented studies. In this regard, some statistical calculations were also realized to evaluate the optimization method performance and detection of optimum values for any data stably and successfully. According to the results, the Jaya algorithm can handle the optimization process successfully, including the case, without grouping truss members. The positive effect of Lèvy flight on swarm-based algorithms can be seen especially for the gray wolf algorithm.
Structured Reporting Using CEUS LI-RADS for the Diagnosis of Hepatocellular Carcinoma (HCC)—Impact and Advantages on Report Integrity, Quality and Interdisciplinary Communication
Authors: Geyer Thomas , Rübenthaler Johannes , Marschner Constantin , von Hake Malte , Fabritius Matthias P. , Froelich Matthias F. , Huber Thomas , Nörenberg Dominik , Rückel Johannes , Weniger Maximilian , Martens Corinna , Sabel Laura , Clevert Dirk-André , Schwarze Vincent
Published: January 2021

Background: Our retrospective single-center study aims to evaluate the impact of structured reporting (SR) using a CEUS LI-RADS template on report quality compared to conventional free-text reporting (FTR) in contrast-enhanced ultrasound (CEUS) for the diagnosis of hepatocellular carcinoma (HCC). Methods: We included 50 patients who underwent CEUS for HCC staging. FTR created after these examinations were compared to SR retrospectively generated by using template-based online software with clickable decision trees. The reports were evaluated regarding report completeness, information extraction, linguistic quality and overall report quality by two readers specialized in internal medicine and visceral surgery. Results: SR significantly increased report completeness with at least one key feature missing in 31% of FTR vs. 2% of SR (p < 0.001). Information extraction was considered easy in 98% of SR vs. 86% of FTR (p = 0.004). The trust of referring physicians in the report was significantly increased by SR with a mean of 5.68 for SR vs. 4.96 for FTR (p < 0.001). SR received significantly higher ratings regarding linguistic quality (5.79 for SR vs. 4.83 for FTR (p < 0.001)) and overall report quality (5.75 for SR vs. 5.01 for FTR (p < 0.001)). Conclusions: Using SR instead of conventional FTR increases the overall quality of reports in CEUS examinations of HCC patients and may represent a valuable tool to facilitate clinical decision-making and improve interdisciplinary communication in the future.