bluuebunny/crossref_metadata_2025 huggingface dataset:
Mesencephalic Locomotor Region and Presynaptic Inhibition during Anticipatory Postural Adjustments in People with Parkinson’s Disease
Authors: Silva-Batista Carla , Lira Jumes , Coelho Daniel Boari , de Lima-Pardini Andrea Cristina , Nucci Mariana Penteado , Mattos Eugenia Casella Tavares , Magalhaes Fernando Henrique , Barbosa Egberto Reis , Teixeira Luis Augusto , Amaro Junior Edson , Ugrinowitsch Carlos , Horak Fay B.
Published: February 2024

Individuals with Parkinson’s disease (PD) and freezing of gait (FOG) have a loss of presynaptic inhibition (PSI) during anticipatory postural adjustments (APAs) for step initiation. The mesencephalic locomotor region (MLR) has connections to the reticulospinal tract that mediates inhibitory interneurons responsible for modulating PSI and APAs. Here, we hypothesized that MLR activity during step initiation would explain the loss of PSI during APAs for step initiation in FOG (freezers). Freezers (n = 34) were assessed in the ON-medication state. We assessed the beta of blood oxygenation level-dependent signal change of areas known to initiate and pace gait (e.g., MLR) during a functional magnetic resonance imaging protocol of an APA task. In addition, we assessed the PSI of the soleus muscle during APA for step initiation, and clinical (e.g., disease duration) and behavioral (e.g., FOG severity and APA amplitude for step initiation) variables. A linear multiple regression model showed that MLR activity (R2 = 0.32, p = 0.0006) and APA amplitude (R2 = 0.13, p = 0.0097) explained together 45% of the loss of PSI during step initiation in freezers. Decreased MLR activity during a simulated APA task is related to a higher loss of PSI during APA for step initiation. Deficits in central and spinal inhibitions during APA may be related to FOG pathophysiology.
Environmental Refuges during Summertime Heat and Elevated Ozone Levels: A Preliminary Case Study of an Urban “Cool Zone” Building
Authors: Mendoza Daniel L. , Crosman Erik T. , Anderson Corbin , Gonzales Shawn A.
Published: February 2024

The combination of extreme heat waves and ozone pollution is a major health hazard for urban populations in the summertime, particularly for the most sensitive groups such as children, the elderly, the unsheltered, and those with pre-existing health conditions. The “Cool Zone Program”, operated by the Salt Lake County Aging and Adult Services, identifies areas in the county and Salt Lake City facilities where members of the public can escape the summer heat, hydrate, and learn about available programs. We measured indoor and outdoor temperature and ozone for a pilot study at a designated Cool Zone location during the 22 August–6 September 2019 period and found that the building provided substantial heat relief and protection from more than 75% of the outdoor ozone. We observed a nearly 35 min delay for the outdoor ozone to be reflected on the indoor readings, providing an action window for ventilation scheduling changes to protect against the highest ozone levels during the day. Our findings show that it is critical to re-think and formulate action plans to protect vulnerable populations from excessive heat and pollution events during the summer.
Variant Allele Frequency Analysis of Circulating Tumor DNA as a Promising Tool in Assessing the Effectiveness of Treatment in Non-Small Cell Lung Carcinoma Patients
Authors: Galant Natalia , Nicoś Marcin , Kuźnar-Kamińska Barbara , Krawczyk Paweł
Published: February 2024

Despite the different possible paths of treatment, lung cancer remains one of the leading causes of death in oncological patients. New tools guiding the therapeutic process are under scientific investigation, and one of the promising indicators of the effectiveness of therapy in patients with NSCLC is variant allele frequency (VAF) analysis. VAF is a metric characterized as the measurement of the specific variant allele proportion within a genomic locus, and it can be determined using methods based on NGS or PCR. It can be assessed using not only tissue samples but also ctDNA (circulating tumor DNA) isolated from liquid biopsy. The non-invasive characteristic of liquid biopsy enables a more frequent collection of material and increases the potential of VAF analysis in monitoring therapy. Several studies have been performed on patients with NSCLC to evaluate the possibility of VAF usage. The research carried out so far demonstrates that the evaluation of VAF dynamics may be useful in monitoring tumor progression, remission, and recurrence during or after treatment. Moreover, the use of VAF analysis appears to be beneficial in making treatment decisions. However, several issues require better understanding and standardization before VAF testing can be implemented in clinical practice. In this review, we discuss the difficulties in the application of ctDNA VAF analysis in clinical routine, discussing the diagnostic and methodological challenges in VAF measurement in liquid biopsy. We highlight the possible applications of VAF-based measurements that are under consideration in clinical trials in the monitoring of personalized treatments for patients with NSCLC.
Radiation Therapy for Stage IIA/B Seminoma: Modeling Secondary Cancer Risk for Protons and VMAT versus 3D Photons
Authors: Pursley Jennifer , Remillard Kyla , Depauw Nicolas , Lee Grace , Grassberger Clemens , Paganetti Harald , Efstathiou Jason A. , Kamran Sophia C.
Published: February 2024

Radiation therapy (RT) is an effective treatment for stage IIA and select stage IIB seminomas. However, given the long life expectancy of seminoma patients, there are concerns about the risk of secondary cancers from RT. This study assessed differences in secondary cancer risk for stage II seminoma patients following proton pencil-beam scanning (PBS) and photon VMAT, compared to 3D conformal photon RT. Ten seminoma patients, five with a IIA staging who received 30 GyRBE and five with a IIB staging who received 36 GyRBE, had three RT plans generated. Doses to organs at risk (OAR) were evaluated, and secondary cancer risks were calculated as the Excess Absolute Risk (EAR) and Lifetime Attributable Risk (LAR). PBS reduced the mean OAR dose by 60% on average compared to 3D, and reduced the EAR and LAR for all OAR, with the greatest reductions seen for the bowel, liver, and stomach. VMAT reduced high doses but increased the low-dose bath, leading to an increased EAR and LAR for some OAR. PBS provided superior dosimetric sparing of OAR compared to 3D and VMAT in stage II seminoma cases, with models demonstrating that this may reduce secondary cancer risk. Therefore, proton therapy shows the potential to reduce acute and late side effects of RT for this population.
Splenectomy as Part of Maximal-Effort Cytoreductive Surgery in Advanced Epithelial Ovarian Cancer
Authors: Pergialiotis Vasilios , Zachariou Eleftherios , Lygizos Vasilios , Vlachos Dimitrios Efthymios , Stamatakis Emmanouil , Angelou Kyveli , Daskalakis Georgios , Thomakos Nikolaos , Haidopoulos Dimitrios
Published: February 2024

Introduction: A splenectomy is frequently performed during debulking surgery for advanced ovarian cancer. Its impact on perioperative and survival outcomes remains questionable as current evidence is conflicting. In the present study, we sought to determine the factors that affect survival rates in ovarian cancer patients that undergo a splenectomy as part of maximal-effort cytoreduction. Patients and methods: A retrospective chart review was conducted that included all epithelial ovarian cancer patients that had surgical cytoreduction for advanced epithelial ovarian cancer. Differences among splenectomized and non splenectomized patients were evaluated as well as the impact of known risk factors on survival outcomes of splenectomized patients. Results: Overall, 245 patients were identified and 223 were included in the present series, of whom 91 had a splenectomy. Recurrence rates as well as death rates were comparable among splenectomized and non-splenectomized patients; however, both the disease-free survival (log-rank = 0.001), as well as the overall survival of splenectomized patients (log-rank = 0.006), was shorter. Thrombotic events as well as rates of pulmonary embolism were comparable. Sepsis was more common among splenectomized patients. The site of splenic metastases did not influence patients’ survival. Among splenectomized patients, those offered primary debulking had longer progression-free survival (log-rank = 0.042), although their overall survival did not differ compared to patients submitted to interval debulking. Complete debulking significantly improved the overall survival compared to optimal debulking (log-rank = 0.047). Splenectomized patients that developed sepsis had worse overall survival (log-rank = 0.005). Discussion: The findings of our study support the feasibility of splenectomy in advanced epithelial ovarian cancer; however, its impact on patients’ survival is considerable. Therefore, every effort should be made to avoid splenic injury which will result in unintended splenectomy for non-oncological reasons.
Male Fertility and Fatherhood in Chronic Myeloid Leukemia: Current Understanding and Future Perspectives
Authors: Elsabagh Ahmed Adel , Benkhadra Maria , Elmakaty Ibrahim , Elsayed Abdelrahman , Elsayed Basant , Elmarasi Mohamed , Abutineh Mohammad , Qasem Nabeel Mohammad , Ali Elrazi , Yassin Mohamed
Published: February 2024

Chronic myeloid leukemia (CML), while traditionally a disease of the elderly, has recently risen in incidence among younger patients. Hence, fertility concerns have emerged considering the disease process and treatments, especially with the current scarce and conflicting recommendations. This review explores the impact of CML treatments including the first-line tyrosine kinase inhibitors (TKIs) and other treatments on male fertility in chronic myeloid leukemia (CML) patients. The aim of this review was to compile the available evidence on male fertility to ultimately tailor treatment plans for male CML patients for whom fertility and future chances for conception pose a concern. The data available on the conventional and newer TKIs to address fertility concerns were reviewed, particularly the potential long- and short-term effects. Also, the possible side effects on subsequent generations were a crucial focus point of this review to reach a more comprehensive CML management approach. We found and compared the evidence on TKIs approved to treat CML. We also reported the effects of hydroxyurea, interferon, and transplantation, which are considered second-line treatments. Our findings suggest that these drugs might have an undiscovered effect on fertility. More research with larger sample sizes and longer follow-up periods is essential to solidify our understanding of these effects.
Clinical Evaluation of the Pancreatic Cancer Microenvironment: Opportunities and Challenges
Authors: Szczepanski Julianne M. , Rudolf Mark A. , Shi Jiaqi
Published: February 2024

Advances in our understanding of pancreatic ductal adenocarcinoma (PDAC) and its tumor microenvironment (TME) have the potential to transform treatment for the hundreds of thousands of patients who are diagnosed each year. Whereas the clinical assessment of cancer cell genetics has grown increasingly sophisticated and personalized, current protocols to evaluate the TME have lagged, despite evidence that the TME can be heterogeneous within and between patients. Here, we outline current protocols for PDAC diagnosis and management, review novel biomarkers, and highlight potential opportunities and challenges when evaluating the PDAC TME as we prepare to translate emerging TME-directed therapies to the clinic.
Morphological Changes of 3T3 Cells under Simulated Microgravity
Authors: Tran Minh Thi , Ho Chi Nguyen Quynh , Hoang Son Nghia , Doan Chung Chinh , Nguyen Minh Thai , Van Huy Duc , Ly Cang Ngoc , Le Cuong Phan Minh , Hoang Huy Nghia Quang , Nguyen Han Thai Minh , Truong Han Thi , To Quan Minh , Nguyen Tram Thi Thuy , Le Long Thanh
Published: February 2024

Background: Cells are sensitive to changes in gravity, especially the cytoskeletal structures that determine cell morphology. The aim of this study was to assess the effects of simulated microgravity (SMG) on 3T3 cell morphology, as demonstrated by a characterization of the morphology of cells and nuclei, alterations of microfilaments and microtubules, and changes in cycle progression. Methods: 3T3 cells underwent induced SMG for 72 h with Gravite®, while the control group was under 1G. Fluorescent staining was applied to estimate the morphology of cells and nuclei and the cytoskeleton distribution of 3T3 cells. Cell cycle progression was assessed by using the cell cycle app of the Cytell microscope, and Western blot was conducted to determine the expression of the major structural proteins and main cell cycle regulators. Results: The results show that SMG led to decreased nuclear intensity, nuclear area, and nuclear shape and increased cell diameter in 3T3 cells. The 3T3 cells in the SMG group appeared to have a flat form and diminished microvillus formation, while cells in the control group displayed an apical shape and abundant microvilli. The 3T3 cells under SMG exhibited microtubule distribution surrounding the nucleus, compared to the perinuclear accumulation in control cells. Irregular forms of the contractile ring and polar spindle were observed in 3T3 cells under SMG. The changes in cytoskeleton structure were caused by alterations in the expression of major cytoskeletal proteins, including β-actin and α-tubulin 3. Moreover, SMG induced 3T3 cells into the arrest phase by reducing main cell cycle related genes, which also affected the formation of cytoskeleton structures such as microfilaments and microtubules. Conclusions: These results reveal that SMG generated morphological changes in 3T3 cells by remodeling the cytoskeleton structure and downregulating major structural proteins and cell cycle regulators.
Protective Potential of a Botanical-Based Supplement Ingredient against the Impact of Environmental Pollution on Cutaneous and Cardiopulmonary Systems: Preclinical Study
Authors: Peno-Mazzarino Laurent , Radionov Nikita , Merino Marián , González Sonia , Mullor José L. , Jones Jonathan , Caturla Nuria
Published: February 2024

Air pollution is a growing threat to human health. Airborne pollution effects on respiratory, cardiovascular and skin health are well-established. The main mechanisms of air-pollution-induced health effects involve oxidative stress and inflammation. The present study evaluates the potential of a polyphenol-enriched food supplement ingredient comprising Lippia citriodora, Olea europaea, Rosmarinus officinalis, and Sophora japonica extracts in mitigating the adverse effects of environmental pollution on skin and cardiopulmonary systems. Both in vitro and ex vivo studies were used to assess the blend’s effects against pollution-induced damage. In these studies, the botanical blend was found to reduce lipid peroxidation, inflammation (by reducing IL-1α), and metabolic alterations (by regulating MT-1H, AhR, and Nrf2 expression) in human skin explants exposed to a mixture of pollutants. Similar results were also observed in keratinocytes exposed to urban dust. Moreover, the ingredient significantly reduced pollutant-induced ROS production in human endothelial cells and lung fibroblasts, while downregulating the expression of apoptotic genes (bcl-2 and bax) in lung fibroblasts. Additionally, the blend counteracted the effect of urban dust on the heart rate in zebrafish embryos. These results support the potential use of this supplement as an adjuvant method to reduce the impact of environmental pollution on the skin, lungs, and cardiovascular tissues.
Polypyrrole/α-Fe2O3 Hybrids for Enhanced Electrochemical Sensing Performance towards Uric Acid
Authors: Wang Renjie , Liu Shanshan , Song Xudong , Jiang Kai , Hou Yaohui , Cheng Qiaohuan , Miao Wei , Tian Li , Ren Ying , Xu Sankui
Published: February 2024

Uric acid, a metabolite formed by the oxidation of purines in the human body, plays a crucial role in disease development when its metabolism is altered. Various techniques have been employed for uric acid analysis, with electrochemical sensing emerging as a sensitive, selective, affordable, rapid, and simple approach. In this study, we developed a polymer-based sensor (PPy/α-Fe2O3) for the accurate determination of uric acid levels. The PPy/α-Fe2O3 hybrids were synthesized using an uncomplicated in situ growth technique. Characterization of the samples was performed using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The electrochemical sensing performance towards uric acid was evaluated through cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The obtained results demonstrated that the sensor exhibited excellent sensitivity towards uric acid detection within a wide range of 5–200 μM with a limit of detection (LOD) as low as 1.349 μM. Furthermore, this work elucidated the underlying sensing mechanism and highlighted the pivotal role played by PPy/α-Fe2O3 hybrids in enabling efficient uric acid sensing applications using electrochemical sensors.
Status of Marine Debris Damage to Adult and Young Black-Tailed Gulls (Larus crassirostris) in Their Breeding Colonies in South Korea
Authors: Hong Mi-Jin , Yun Seongho , Yang Min-Seung , Jeon Hye-Jeong , Yoo Jeong-Chil , Lee Who-Seung
Published: February 2024

Marine debris from fishing-related paraphernalia poses a threat to the survival of marine organisms, especially seabirds. Although the detrimental effects of marine debris on seabirds have been documented, studies on the extent of damage inflicted by marine debris on the seabird breeding population are scarce. Here, marine debris ingestion and entanglement damage to black-tailed gulls (Larus crassirostris) residing in South Korea were quantified. The five breeding colonies of black-tailed gulls were visited, and the frequency of ingestion and entanglement damage in adults and young were recorded. A total of 25 cases of marine debris damage were confirmed. As a result, damage by marine debris to gulls varied depending on breeding colonies. More adults suffered from entanglement damage than the young, and their most damaged parts were usually their legs. Fishing lines and hooks caused the most damage. We suggest that marine debris damage acquired in breeding colonies could affect breeding success.
Creative Thinking in Art and Design Education: A Systematic Review
Authors: Samaniego Mariela , Usca Nancy , Salguero José , Quevedo William
Published: February 2024

This study aims to identify and analyze relevant characteristics associated with creative thinking, particularly in arts and design education. A systematic literature review was conducted following the PRISMA protocol, utilizing the Scopus database, where 292 studies were retrieved through search strings. From these, 187 studies were selected for the final analysis. The results highlight an emphasis on experiential learning, STEAM (Science, Technology, Engineering, Arts, and Mathematics), and interdisciplinary approaches as prevalent educational methodologies for fostering creative thinking. The identified techniques include interdisciplinary projects, artistic practices, nature-based activities, and the use of digital tools. The core skills identified include originality, fluency, flexibility, and elaboration. Furthermore, it was observed that most of the studies were conducted in higher education institutions. The study underscores the urgency of promoting research in specific regions, such as Latin America, to contribute to advancing and enriching the educational landscape in these areas. Additionally, it emphasizes the importance of fostering creativity from an early age. The significance of this study lies in its contribution to more effective pedagogical practices for the development of creative thinking that positively impacts education and prepares individuals for the challenges of the 21st century.
The Role of Second Language Reading Proficiency in Moderating Second Language Word Recognition
Authors: Li Xiaomeng , Chen Tianxu
Published: February 2024

Drawing upon the division of labor between orthographic and phonological information, this study investigated whether and how L2 reading proficiency moderates learners’ reliance on phonological and orthographic information in retrieving word meanings. A total of 136 Chinese collegiate students who learned English as a foreign language (EFL) completed English reading proficiency tests and were divided into higher and lower reading proficiency groups using an extreme-group approach. Behavioral tasks were used to measure the participants’ sensitivity to and processing skills of orthographic and phonological information. The analysis showed that the reliance on phonological and orthographic information differed significantly across L2 reading proficiency groups: The higher reading proficiency group was sensitive to both phonological and orthographic information within words, while the lower reading proficiency group was only sensitive to orthographic information; only orthographic processing skills significantly contributed to the word meaning retrieval of individuals in the higher reading proficiency group, while phonological processing skills were the only predictor for the lower reading proficiency group. These results suggest that the use of phonological and orthographic information vary as a function of L2 learners’ English reading proficiency. Implications regarding the changing patterns of L1 influences and the language-universal and language-specific aspects of word recognition were discussed.
Research on Multi-DAG Satellite Network Task Scheduling Algorithm Based on Cache-Composite Priority
Authors: Liu Zhiguo , Zhang Luxi , Wang Lin , Dong Xiaoqi , Rong Junlin
Published: February 2024

The problem of multiple DAGs sharing satellite constellation resources has gradually attracted widespread attention. Due to the limited computing resources and energy consumption of satellite networks, it is necessary to formulate a reasonable multi-DAG task scheduling scheme to ensure the fairness of each workflow under the premise of considering latency and energy consumption. Therefore, in this paper, we propose a multi-DAG satellite network task scheduling algorithm based on cache-composite priority under the Software-Defined Networking satellite network architecture. The basic idea of this algorithm lies in the DAG selection phase, where not only are task priorities computed but also the concept of fair scheduling is introduced, so as to prevent the excessively delayed scheduling of low-priority DAG tasks. In addition, the concept of public subtasks is introduced to reduce the system overhead caused by repetitive tasks. The experimental results show that the hybrid scheduling strategy proposed in this paper can meet the demand of DAG scheduling and improve the degree of task completion while effectively reducing the task latency and energy consumption.
A Triboelectric Nanogenerator Based on Bamboo Leaf for Biomechanical Energy Harvesting and Self-Powered Touch Sensing
Authors: Xu Zhantang , Chang Yasheng , Zhu Zhiyuan
Published: February 2024

Recently, natural material-based triboelectric nanogenerators (TENGs) have increasingly attracted attention in academic circles. In this work, we have developed an innovative triboelectric nanogenerator (BL-TENG) utilizing bamboo leaves to capture biomechanical energy. Bamboo leaf, as a natural plant material, possesses a diverse array of applications due to its remarkable durability, which surpasses that of many other types of trees. Furthermore, bamboo leaf has the advantages of low cost, widely distributed, non-toxic and environmentally protected. The output power of the BL-TENG (size: 5 cm × 5 cm) is able to generate approximately 409.6 µW and the internal resistance of the BL-TENG is 40 MΩ. Furthermore, the BL-TENG can realize an open-circuit voltage (Voc) of 191 V and a short-circuit current (Isc) of 5 µA, respectively. The biomechanical energy harvesting effect of the BL-TENG device means that it can drive 18 commercial light-emitting diodes (LEDs) through the full-wave bridge rectifier. Furthermore, the BL-TENG can also serve as a self-powered touch sensor to reflect hand touch states. This study proposed a novel plant-based TENG device that can enhance the development of green TENG devices and self-powered sensing systems.
Synchronizing TSN Devices via 802.1AS over 5G Networks
Authors: Muslim Anas Bin , Tönjes Ralf , Bauschert Thomas
Published: February 2024

The 3GPP release 16 integrates TSN functionality into 5G and standardizes various options for TSN time synchronization over 5G such as transparent mode and bridge mode. The time domains for the TSN network and the 5G network are kept separate with an option to synchronize either of the networks to the other. The TSN time synchronization over 5G is possible either by using the IEEE 1588 generalized Precision Time Protocol (gPTP) based on UDP/IP multicast or via IEEE 802.1AS based on Ethernet PDUs. The INET and Simu5G simulation frameworks, which are both based on the OMNeT++ discrete event simulator, are widely used for simulating TSN and 5G networks. The INET framework comprises the 802.1AS based time synchronization mechanism, and Simu5G provides the 5G user plane carrying IP PDUs. We modified the 802.1AS-based synchronization model of INET so that it works over UDP/IP. With that, it is possible to synchronize TSN slaves (connected to 5G UEs), across a 5G network, with a TSN master clock, present within a TSN network, that is connected to the 5G core network. Our simulation results show that 500 microseconds of synchronization accuracy can be achieved with the corrected asymmetric propagation delay of uplink and downlink between the gNodeB (gNB) and the User Equipment (UE). Furthermore, the synchronization accuracy can be improved if the delay difference between uplink and downlink is known.
Design and Implementation of a UMLRPAsec-Extension for Robotic Process Automation
Authors: Kurylets Anastasiya , Goranin Nikolaj
Published: February 2024

Ensuring RPA (robotic process automation) security is a critical aspect when developing and operating automated software robots. One of the key steps for developing secure software robots is the design stage: the identification and specification of the requirements for the security of the system, the description of system precedents, the interaction between the classes involved in the robot being created, etc. Designs using security-oriented formal modeling languages, such as the UMLsec extension of UML, involve not only a visual representation of diagrams but also the possibility to focus the attention on security issues. However, currently, in the scientific community, there is no possibility of using stereotypes specialized for robots—a mechanism for expanding the unified modeling language that would explicitly reflect a specific problem in the subject area. In this article, we propose that the UMLRPAsec-extension for RPA can be used to model security in the RPA context.
Analysis of a Graphene FET-Based Frequency Doubler for Combined Sensing and Modulation through Compact Model Simulation
Authors: La Mura Monica , Lamberti Patrizia , Tucci Vincenzo
Published: February 2024

The ambipolar conduction property of graphene field-effect transistors (GFETs) and the inherent square-like dependence of the drain current on the gate voltage, enable the development of single-device architectures for analog nonlinear radiofrequency (RF) circuits. The use of GFETs in novel RF component topologies allows leveraging graphene’s attractive thermal and mechanical properties to improve the miniaturization and weight reduction of electronic components. These features are specifically appealing for integrated sensing, modulation, and transmission systems. However, given the innovative nature of emerging graphene-based technology, a complete performance analysis of any novel electronic component is essential for customizing the operating conditions accordingly. This paper presents a comprehensive circuital analysis of a GFET-based frequency doubler, exploiting a compact model for GFET circuit simulation to assess the device’s performance parameters, including power conversion gain bandwidth and saturation. The performed analysis proposes to support the design of GFET-based harmonic transponders, offering integrated sensing and signal manipulation capabilities.
A Crypto Yield Model for Staking Return
Authors: Riposo Julien , Gupta Maneesh
Published: February 2024

We introduce a model that derives a metric to answer the question: what is the expected gain of a staker? We calculate the rewards as the staking return in a Proof-of-Stake (PoS) consensus context. For each period of block validation and by a forward approach, we prove that the interest is given by the ratio of the average staking gain to the total staked coins. Some additional PoS features are considered in the model, such as slash rate and Maximal Extractable Value (MEV), which marks the originality of this approach. In particular, we prove that slashing diminishes the rewards, reflecting the fact that the blockchain can consider stakers to potentially validate incorrectly. Regarding MEV, the approach we have sheds light on the relation between transaction fees and the average staking gain. We illustrate the developed model with Ethereum 2.0 and apply a similar process in a Proof-of-Work consensus context.
The Role of Financial Sanctions and Financial Development Factors on Central Bank Digital Currency Implementation
Authors: Mohammed Medina Ayta , De-Pablos-Heredero Carmen , Montes Botella José Luis
Published: February 2024

This study investigates the influence of a country’s financial access and stability and the adoption of retail central bank digital currencies (CBDCs) across 71 countries. Using an ordinal logit model, we examine how individual financial access, the ownership of credit cards, financing accessibility by firms, offshore loans, financial sanctions, and the ownership structure of financial institutions influence the probability of CBDC adoption in nations. These findings reveal that nations facing financial sanctions and those with substantial offshore bank loans are more inclined to adopt CBDCs. Furthermore, a significant relationship is observed in countries where many people have restricted financial access, indicating heightened interest in CBDC adoption. Interestingly, no statistically significant relationship was found between the adoption of CBDCs and the percentage of foreign-owned banks in each country. The results show that countries with low financial stability and financial access adopt CBDCs faster. This study expands our knowledge of how a nation’s financial situation influences its adoption of CBDCs. The results provide important and relevant insights into the current discussion of the direction of global finance.
Xenopus Sox11 Partner Proteins and Functional Domains in Neurogenesis
Authors: Singleton Kaela S. , Silva-Rodriguez Pablo , Cunningham Doreen D. , Silva Elena M.
Published: February 2024

Sox11, a member of the SoxC family of transcription factors, has distinct functions at different times in neural development. Studies in mouse, frog, chick, and zebrafish show that Sox11 promotes neural fate, neural differentiation, and neuron maturation in the central nervous system. These diverse roles are controlled in part by spatial and temporal-specific protein interactions. However, the partner proteins and Sox11-interaction domains underlying these diverse functions are not well defined. Here, we identify partner proteins and the domains of Xenopus laevis Sox11 required for protein interaction and function during neurogenesis. Our data show that Sox11 co-localizes and interacts with Pou3f2 and Neurog2 in the anterior neural plate and in early neurons, respectively. We also demonstrate that Sox11 does not interact with Neurog1, a high-affinity partner of Sox11 in the mouse cortex, suggesting that Sox11 has species-specific partner proteins. Additionally, we determined that the N-terminus including the HMG domain of Sox11 is necessary for interaction with Pou3f2 and Neurog2, and we established a novel role for the N-terminal 46 amino acids in the specification of placodal progenitors. This is the first identification of partner proteins for Sox11 and of domains required for partner-protein interactions and distinct roles in neurogenesis.
Metabolic Endoscopy: Should Gastroenterologists Be Treating Type 2 Diabetes?
Authors: Norton Benjamin Charles , Papaefthymiou Apostolis , Telese Andrea , Mallik Ritwika , Murino Alberto , Johnson Gavin , Murray Charles , Makaronidis Janine , Haidry Rehan
Published: February 2024

Type 2 diabetes (T2D) is one of the most significant and fast-growing health challenges of the 21st century. Despite the variety of available glucose-lowering agents, many patients do not attain or maintain adequate glycaemic control. Bariatric surgery demonstrates a profound anti-diabetic effect, which is almost immediate and weight-loss independent. The significant improvement in diabetes after bariatric surgery together with the endoscopic accessibility of the duodenum has led to the development of new metabolic endoscopic procedures that capitalise on the importance of the proximal small bowel in glycaemic control. Clinical trials have shown a clear efficacy signal, and now, several devices are undergoing evaluation as primary T2D treatments. Establishing where these procedures fit into the treatment algorithms for T2D and how they can be combined with modern pharmacotherapies is needed in a rapidly changing landscape. Ultimately, through metabolic endoscopy, gastroenterologists are on the cusp of providing safe and effective treatments for the multidisciplinary management of T2D.
Comet Assay: Multifaceted Options for Studies of Plant Stress Response
Authors: Tyutereva Elena V. , Strizhenok Aleksei D. , Kiseleva Elizaveta I. , Voitsekhovskaja Olga V.
Published: February 2024

Contrarily to chronic stresses, acute (i.e., fast and dramatic) changes in environmental factors like temperature, radiation, concentration of toxic substances, or pathogen attack often lead to DNA damage. Some of the stress factors are genotoxic, i.e., they damage the DNA via physical interactions or via interference with DNA replication/repair machinery. However, cytotoxic factors, i.e., those that do not directly damage the DNA, can lead to secondary genotoxic effects either via the induction of the production of reactive oxygen, carbon, or nitrogen species, or via the activation of programmed cell death and related endonucleases. The extent of this damage, as well as the ability of the cell to repair it, represent a significant part of plant stress responses. Information about DNA damage is important for physiological studies as it helps to understand the complex adaptive responses of plants and even to predict the outcome of the plant’s exposure to acute stress. Single cell gel electrophoresis (Comet assay) provides a convenient and relatively inexpensive tool to evaluate DNA strand breaks in the different organs of higher plants, as well as in unicellular algae. Comet assays are widely used in ecotoxicology and biomonitoring applications; however, they are still relatively rarely used in physiological studies. In this review, we provide an overview of the basic principles and of useful variations of the protocols of Comet assays, as well as of their use in plant studies, in order to encourage plant physiologists to include this tool in the analysis of plant stress responses.
The Facile Synthesis and Application of Mesoporous Silica Nanoparticles with a Vinyl Functional Group for Plastic Recycling
Authors: Lee Jong-tak , Kang Misun , Bae Jae Young
Published: February 2024

Due to growing concerns about environmental pollution from plastic waste, plastic recycling research is gaining momentum. Traditional methods, such as incorporating inorganic particles, increasing cross-linking density with peroxides, and blending with silicone monomers, often improve mechanical properties but reduce flexibility for specific performance requirements. This study focuses on synthesizing silica nanoparticles with vinyl functional groups and evaluating their mechanical performance when used in recycled plastics. Silica precursors, namely sodium silicate and vinyltrimethoxysilane (VTMS), combined with a surfactant, were employed to create pores, increasing silica’s surface area. The early-stage introduction of vinyl functional groups prevented the typical post-synthesis reduction in surface area. Porous silica was produced in varying quantities of VTMS, and the synthesized porous silica nanomaterials were incorporated into recycled polyethylene to induce cross-linking. Despite a decrease in surface area with increasing VTMS content, a significant surface area of 883 m2/g was achieved. In conclusion, porous silica with the right amount of vinyl content exhibited improved mechanical performance, including increased tensile strength, compared to conventional porous silica. This study shows that synthesized porous silica with integrated vinyl functional groups effectively enhances the performance of recycled plastics.
Antioxidant Efficacy of Hwangryunhaedok-tang through Nrf2 and AMPK Signaling Pathway against Neurological Disorders In Vivo and In Vitro
Authors: Bae Su-Jin , Lee Won-Yung , Bak Seon Been , Lee Seung Jin , Hwang Su-Jin , Kim Geun-Woo , Koo Byung-Soo , Park Sun-Dong , Yoo Hye-Hyun , Kim Choon-Ok , Kang Hyung Won , Oh Tae-Woo , Kim Young Woo
Published: February 2024

Alzheimer’s disease (AD) is a representative cause of dementia and is caused by neuronal loss, leading to the accumulation of aberrant neuritic plaques and the formation of neurofibrillary tangles. Oxidative stress is involved in the impaired clearance of amyloid beta (Aβ), and Aβ-induced oxidative stress causes AD by inducing the formation of neurofibrillary tangles. Hwangryunhaedok-tang (HHT, Kracie K-09®), a traditional herbal medicine prescription, has shown therapeutic effects on various diseases. However, the studies of HHT as a potential treatment for AD are insufficient. Therefore, our study identified the neurological effects and mechanisms of HHT and its key bioactive compounds against Alzheimer’s disease in vivo and in vitro. In a 5xFAD mouse model, our study confirmed that HHT attenuated cognitive impairments in the Morris water maze (MWM) test and passive avoidance (PA) test. In addition, the prevention of neuron impairment, reduction in the protein levels of Aβ, and inhibition of cell apoptosis were confirmed with brain tissue staining. In HT-22 cells, HHT attenuates tBHP-induced cytotoxicity, ROS generation, and mitochondrial dysfunction. It was verified that HHT exerts a neuroprotective effect by activating signaling pathways interacting with Nrf2, such as MAPK/ERK, PI3K/Akt, and LKB1/AMPK. Among the components, baicalein, a bioavailable compound of HHT, exhibited neuroprotective properties and activated the Akt, AMPK, and Nrf2/HO-1 pathways. Our findings indicate a mechanism for HHT and its major bioavailable compounds to treat and prevent AD and suggest its potential.
Differential Expression of Stress Adaptation Genes in a Diatom Ulnaria acus under Different Culture Conditions
Authors: Bayramova Elvira , Petrova Darya , Marchenkov Artyom , Morozov Alexey , Galachyants Yuri , Zakharova Yulia , Bedoshvili Yekaterina , Likhoshway Yelena
Published: February 2024

Diatoms are a group of unicellular eukaryotes that are essential primary producers in aquatic ecosystems. The dynamic nature of their habitat necessitates a quick and specific response to various stresses. However, the molecular mechanisms of their physiological adaptations are still underexplored. In this work, we study the response of the cosmopolitan freshwater diatom Ulnaria acus (Bacillariophyceae, Fragilariophycidae, Licmophorales, Ulnariaceae, Ulnaria) in relation to a range of stress factors, namely silica deficiency, prolonged cultivation, and interaction with an algicidal bacterium. Fluorescent staining and light microscopy were used to determine the physiological state of cells under these stresses. To explore molecular reactions, we studied the genes involved in the stress response—type III metacaspase (MC), metacaspase-like proteases (MCP), death-specific protein (DSP), delta-1-pyrroline-5-carboxylate dehydrogenase (ALDH12), and glutathione synthetase (GSHS). We have described the structure of these genes, analyzed the predicted amino acid sequences, and measured their expression dynamics in vitro using qRT-PCR. We demonstrated that the expression of UaMC1, UaMC3, and UaDSP increased during the first five days of silicon starvation. On the seventh day, it was replaced with the expression of UaMC2, UaGSHS, and UaALDH. After 45 days of culture, cells stopped growing, and the expression of UaMC1, UaMC2, UaGSHS, and UaDSP increased. Exposure to an algicidal bacterial filtrate induced a higher expression of UaMC1 and UaGSHS. Thus, we can conclude that these proteins are involved in diatoms’ adaptions to environmental changes. Further, these data show that the molecular adaptation mechanisms in diatoms depend on the nature and exposure duration of a stress factor.
High Frequencies of Genetic Variants in Patients with Atypical Femoral Fractures
Authors: del Real Álvaro , Cruz Raquel , Sañudo Carolina , Pérez-Castrillón José L. , Pérez-Núñez María I. , Olmos Jose M. , Hernández José L. , García-Ibarbia Carmen , Valero Carmen , Riancho Jose A.
Published: February 2024

This study explores the genetic factors associated with atypical femoral fractures (AFF), rare fractures associated with prolonged anti-resorptive therapy. AFF are fragility fractures that typically appear in the subtrochanteric or diaphyseal regions of the femur. While some cases resemble fractures in rare genetic bone disorders, the exact cause remains unclear. This study investigates 457 genes related to skeletal homeostasis in 13 AFF patients by exome sequencing, comparing the results with osteoporotic patients (n = 27) and Iberian samples from the 1000 Genomes Project (n = 107). Only one AFF case carried a pathogenic variant in the gene set, specifically in the ALPL gene. The study then examined variant accumulation in the gene set, revealing significantly more variants in AFF patients than in osteoporotic patients without AFF (p = 3.7 × 10−5), particularly in ACAN, AKAP13, ARHGEF3, P4HB, PITX2, and SUCO genes, all of them related to osteogenesis. This suggests that variant accumulation in bone-related genes may contribute to AFF risk. The polygenic nature of AFF implies that a complex interplay of genetic factors determines the susceptibility to AFF, with ACAN, SUCO, AKAP13, ARHGEF3, PITX2, and P4HB as potential genetic risk factors. Larger studies are needed to confirm the utility of gene set analysis in identifying patients at high risk of AFF during anti-resorptive therapy.
Detailed Characterization of the Lung–Gut Microbiome Axis Reveals the Link between PD-L1 and the Microbiome in Non-Small-Cell Lung Cancer Patients
Authors: Ankudavicius Vytautas , Nikitina Darja , Lukosevicius Rokas , Tilinde Deimante , Salteniene Violeta , Poskiene Lina , Miliauskas Skaidrius , Skieceviciene Jurgita , Zemaitis Marius , Kupcinskas Juozas
Published: February 2024

Next-generation sequencing technologies have started a new era of respiratory tract research in recent years. Alterations in the respiratory microbiome between healthy and malignant conditions have been revealed. However, the composition of the microbiome varies among studies, even in similar medical conditions. Also, there is a lack of complete knowledge about lung–gut microbiome interactions in lung cancer patients. The aim of this study was to explore the lung–gut axis in non-small-cell lung cancer (NSCLC) patients and the associations between lung–gut axis microbiota and clinical parameters (CRP, NLR, LPS, CD8, and PD-L1). Lung tissue and fecal samples were used for bacterial 16S rRNA sequencing. The results revealed, for the first time, that the bacterial richness in lung tumor tissue gradually decreased with an increase in the level of PD-L1 expression (p < 0.05). An analysis of β-diversity indicated a significant positive correlation between the genera Romboutsia and Alistipes in both the lung tumor biopsies and stool samples from NSCLC patients (p < 0.05). Survival analysis showed that NSCLC patients with higher bacterial richness in their stool samples had prolonged overall survival (HR: 2.06, 95% CI: 1.025–4.17, p = 0.0426).
Impact of Cytomegalovirus (CMV) on an Academic Pediatric Infectious Diseases Outpatient Clinic Referral Population, 2005–2020: Will the Advent of Universal Congenital CMV (cCMV) Screening Change Clinical Practice Referral Patterns?
Authors: Rypka Katelyn J. , Schleiss Mark R.
Published: February 2024

Cytomegalovirus (CMV) infections exert a substantial impact on the practice of pediatric infectious diseases. Although most infections in children are minimally symptomatic, several populations are at risk for CMV-associated disease, including immunosuppressed children, children with HIV infection, and, most significantly, children with congenital CMV (cCMV) infection. In spite of the ubiquitous nature of CMV infection, few studies have quantified the impact of CMV-associated care in a pediatric outpatient clinic setting. We evaluated the impact of CMV on clinical care in an outpatient clinic setting over a fifteen-year period at the University of Minnesota (UMN) Masonic Children’s Hospital Pediatric Infectious Diseases (PID) Clinic. A retrospective review of clinic appointments identified 253 unique patients specifically evaluated over this time period for consideration of CMV infection. Of these, 242 were pediatric patients. The majority of the pediatric patients evaluated in the PID clinic were referred for either confirmed or suspected cCMV infection, including children referred for consideration of CMV as a potential reason for a failed newborn hearing screen (NHS) and/or for evaluation of CMV as a possible etiology for documented hearing loss. In total, 116 of the children evaluated during this time period (48%) were unequivocally confirmed as having cCMV infection, with an additional 37 (15%) presenting with presumed, probable, or possible cCMV infection. A total of 16 (7%) of the pediatric CMV cases were confirmed to be post-natally acquired infections. Of the 253 total patients, 11 (4%) of the referrals were for pregnant patients seeking advice about potential therapies in the setting of a known or suspected primary maternal infection during their pregnancies, with an attendant risk of fetal CMV infection. This overview of the demographics and referral patterns for patients evaluated for known or suspected CMV infections in a tertiary care center outpatient PID clinic will serve as a useful baseline assessment, even as future patterns of outpatient care are highly likely to evolve. We predict that PID clinic referrals for newborns identified by universal cCMV screening programs will result in a shift of the CMV outpatient population to healthier infants with clinically inapparent infections, and care will need to be taken by practitioners not to over-medicalize management for these asymptomatic newborns.