bluuebunny/crossref_metadata_2025 huggingface dataset:
Health Literacy and Active Transport in Austria: Results from a Rural Setting
Authors: Hofer-Fischanger Kathrin , Fuchs-Neuhold Bianca , Müller Alexander , Grasser Gerlinde , van Poppel Mireille N.M.
Published: February 2020

Health literacy (HL) has been determined for the general population and for subgroups, though the relationship between HL and active transport in rural areas was not explored. The aim of our study is to investigate HL among citizens in an Austrian rural region and to explore the associations between HL and active transport. This cross-sectional telephone survey included 288 adults (171 women) with a mean age of 57.8 (SD 0.9). HL was assessed using the HLS-EU-Q16 questionnaire. Active transport was measured as the minutes per week spent on walking or cycling from A to B. After descriptive analysis, the association between HL and active transport was assessed using linear regression models. The mean HL score for all participants was 37.1 (SD 7.7). Among all subjects, 6.9% showed inadequate HL, 25.7% problematic HL, 38.9% sufficient HL, and 28.5% excellent HL. HL was significantly higher among citizens with high education (p = 0.04) and training/employment in healthcare (p = 0.001). Active transport was not associated with HL (p = 0.281). Active transport in rural areas might be influenced by other predictors like distance to work, street connectivity, and accessible facilities for walking and biking. This needs to be explored further for rural areas.
Space-Time Clustering Characteristics of Tuberculosis in Khyber Pakhtunkhwa Province, Pakistan, 2015–2019
Authors: Ullah Sami , Daud Hanita , Dass Sarat , Fanaee-T Hadi , Kausarian Husnul , Alamgir
Published: February 2020

The number of tuberculosis (TB) cases in Pakistan ranks fifth in the world. The National TB Control Program (NTP) has recently reported more than 462,920 TB patients in Khyber Pakhtunkhwa province, Pakistan from 2002 to 2017. This study aims to identify spatial and space-time clusters of TB cases in Khyber Pakhtunkhwa province Pakistan during 2015–2019 to design effective interventions. The spatial and space-time cluster analyses were conducted at the district-level based on the reported TB cases from January 2015 to April 2019 using space-time scan statistics (SaTScan). The most likely spatial and space-time clusters were detected in the northern rural part of the province. Additionally, two districts in the west were detected as the secondary space-time clusters. The most likely space-time cluster shows a tendency of spread toward the neighboring districts in the central part, and the most likely spatial cluster shows a tendency of spread toward the neighboring districts in the south. Most of the space-time clusters were detected at the start of the study period 2015–2016. The potential TB clusters in the remote rural part might be associated to the dry–cool climate and lack of access to the healthcare centers in the remote areas.
Determination of Propane-1,3-sultone in Workplace Air for Occupational Exposure Assessment
Authors: Jeżewska Anna
Published: February 2020

Propane-1,3-sultone (PS) is an alkylating substance used in the production of polymers, fungicides, insecticides, dyes, and detergents. It is absorbed into the human body by inhalation, digestion, and through the skin; it is also a possible carcinogen. Occupational exposure to this substance may occur on industrial or laboratory contact. In Poland, the maximum allowable concentration (MAC) for PS in workplace air is 7 µg/m3. The paper presents a method for determination of PS in workplace air using a gas chromatograph coupled with a mass spectrometer (GC-MS). Air containing PS is passed through a glass tube containing a glass fiber filter and two layers of silica gel. The substance is washed with acetonitrile and the solution obtained analysed using GC-MS. The measuring range for an air sample of 360 L is 0.7 ÷ 14 µg/m3. The limit of detection (LOD) is 13 ng/m3, limit of quantification (LOQ) is 40 ng/m3.
Assessing Similarities and Differences between Males and Females in Visual Behaviors in Spatial Orientation Tasks
Authors: Dong Weihua , Zhan Zhicheng , Liao Hua , Meng Liqiu , Liu Jiping
Published: February 2020

Spatial orientation is an important task in human wayfinding. Existing research indicates sex-related similarities and differences in performance and strategies when executing spatial orientation behaviors, but few studies have investigated the similarities and differences in visual behaviors between males and females. To address this research gap, we explored visual behavior similarities and differences between males and females using an eye-tracking method. We recruited 40 participants to perform spatial orientation tasks in a desktop environment and recorded their eye-tracking data during these tasks. The results indicate that there are no significant differences between sexes in efficiency and accuracy of spatial orientation. In terms of visual behaviors, we found that males fixated significantly longer than females on roads. Males and females had similar fixation counts in building, signpost, map, and other objects. Males and females performed similarly in fixation duration for all five classes. Moreover, fixation duration was well fitted to an exponential function for both males and females. The base of the exponential function fitted by males’ fixation duration was significantly lower than that of females, and the coefficient difference of exponential function was not found. Females were more effective in switching from maps to signposts, but differences of switches from map to other classes were not found. The newfound similarities and differences between males and females in visual behavior may aid in the design of better human-centered outdoor navigation applications.
Complexity Level of People Gathering Presentation on an Animated Map—Objective Effectiveness Versus Expert Opinion
Authors: Medyńska-Gulij Beata , Wielebski Łukasz , Halik Łukasz , Smaczyński Maciej
Published: February 2020

The aim of the following study was to present three alternative methods of visualization on animated maps illustrating the movement of people gathered at an open-air event recorded on photographs taken by a drone. The effectiveness of an orthorectified low-level aerial image (a so-called orthophoto), a dot distribution map, and a buffer map was tested in an experiment featuring experts, and key significance was attached to the juxtaposition of objective responses with subjective opinions. The results of the study enabled its authors to draw conclusions regarding the importance of visualizing topographic references (stable objects) and people (mobile objects) and the usefulness of the particular elements of animated maps for their analysis and interpretation.
Fusion of Sentinel-1 with Official Topographic and Cadastral Geodata for Crop-Type Enriched LULC Mapping Using FOSS and Open Data
Authors: Hütt Christoph , Waldhoff Guido , Bareth Georg
Published: February 2020

Accurate crop-type maps are urgently needed as input data for various applications, leading to improved planning and more sustainable use of resources. Satellite remote sensing is the optimal tool to provide such data. Images from Synthetic Aperture Radar (SAR) satellite sensors are preferably used as they work regardless of cloud coverage during image acquisition. However, processing of SAR is more complicated and the sensors have development potential. Dealing with such a complexity, current studies should aim to be reproducible, open, and built upon free and open-source software (FOSS). Thereby, the data can be reused to develop and validate new algorithms or improve the ones already in use. This paper presents a case study of crop classification from microwave remote sensing, relying on open data and open software only. We used 70 multitemporal microwave remote sensing images from the Sentinel-1 satellite. A high-resolution, high-precision digital elevation model (DEM) assisted the preprocessing. The multi-data approach (MDA) was used as a framework enabling to demonstrate the benefits of including external cadastral data. It was used to identify the agricultural area prior to the classification and to create land use/land cover (LULC) maps which also include the annually changing crop types that are usually missing in official geodata. All the software used in this study is open-source, such as the Sentinel Application Toolbox (SNAP), Orfeo Toolbox, R, and QGIS. The produced geodata, all input data, and several intermediate data are openly shared in a research database. Validation using an independent validation dataset showed a high overall accuracy of 96.7% with differentiation into 11 different crop-classes.
The Indoor Localization of a Mobile Platform Based on Monocular Vision and Coding Images
Authors: Liu Fei , Zhang Jixian , Wang Jian , Li Binghao
Published: February 2020

With the extensive development and utilization of urban underground space, coal mines, and other indoor areas, the indoor positioning technology of these areas has become a hot research topic. This paper proposes a robust localization method for indoor mobile platforms. Firstly, a series of coding graphics were designed for localizing the platform, and the spatial coordinates of these coding graphics were calculated by using a new method proposed in this paper. Secondly, two spatial resection models were constructed based on unit weight and Tukey weight to localize the platform in indoor environments. Lastly, the experimental results show that both models can calculate the position of the platform with good accuracy. The space resection model based on Tukey weight correctly identified the residuals of the observations for calculating the weights to obtain robust positioning results and has a high positioning accuracy. The navigation and positioning method proposed in this study has a high localization accuracy and can be potentially used in localizing practical indoor space mobile platforms.
Automatic Threat Detection for Historic Buildings in Dark Places Based on the Modified OptD Method
Authors: Błaszczak-Bąk Wioleta , Suchocki Czesław , Janicka Joanna , Dumalski Andrzej , Duchnowski Robert , Sobieraj-Żłobińska Anna
Published: February 2020

Historic buildings, due to their architectural, cultural, and historical value, are the subject of preservation and conservatory works. Such operations are preceded by an inventory of the object. One of the tools that can be applied for such purposes is Light Detection and Ranging (LiDAR). This technology provides information about the position, reflection, and intensity values of individual points; thus, it allows for the creation of a realistic visualization of the entire scanned object. Due to the fact that LiDAR allows one to ‘see’ and extract information about the structure of an object without the need for external lighting or daylight, it can be a reliable and very convenient tool for data analysis for improving safety and avoiding disasters. The main goal of this paper is to present an approach of automatic wall defect detection in unlit sites by means of a modified Optimum Dataset (OptD) method. In this study, the results of Terrestrial Laser Scanning (TLS) measurements conducted in two historic buildings in rooms without daylight are presented. One location was in the basement of the ruins of a medieval tower located in Dobre Miasto, Poland, and the second was in the basement of a century-old building located at the University of Warmia and Mazury in Olsztyn, Poland. The measurements were performed by means of a Leica C-10 scanner. The acquired dataset of x, y, z, and intensity was processed by the OptD method. The OptD operates in such a way that within the area of interest where surfaces are imperfect (e.g., due to cracks and cavities), more points are preserved, while at homogeneous surfaces (areas of low interest), more points are removed (redundant information). The OptD algorithm was additionally modified by introducing options to detect and segment defects on a scale from 0 to 3 (0—harmless, 1—to the inventory, 2—requiring repair, 3—dangerous). The survey results obtained proved the high effectiveness of the modified OptD method in the detection and segmentation of the wall defects. The values of area of changes were calculated. The obtained information about the size of the change can be used to estimate the costs of repair, renovation, and reconstruction.
A New Approach to Refining Land Use Types: Predicting Point-of-Interest Categories Using Weibo Check-in Data
Authors: Zhang Xucai , Sun Yeran , Zheng Anyao , Wang Yu
Published: February 2020

The information of land use plays an important role in urban planning and optimizing the allocation of resources. However, traditional land use classification is imprecise. For instance, the type of commercial land is highly filled with the categories of shopping, eating, etc. The number of mixed-use lands is increasingly growing nowadays, and these lands sometimes are too mixed to be well investigated by conventional approaches such as remote sensing technology. To address this issue, we used a new social sensing approach to classify land use according to human mobility and activity patterns. Previous studies used other social sensing approaches to predict land use types at the parcel or the area level, whilst fine-grained point-of-interest (POI)-level land use data are likely to more useful in urban planning. To abridge this research gap, we proposed a new social sensing approach dedicated to classifying land use at a finer scale (i.e., POI-level or building level) according to human mobility and activity patterns reflected by location-based social network (LBSN) data. Specifically, we firstly investigated spatial and temporal patterns of human mobility and activity behavior using check-in data from a popular Chinese LBSN named Sina Weibo and subsequently applied those patterns to predicting the category of POI to refine urban land use classification in Guangzhou, China. In this study, we applied three classification methods (i.e., naive Bayes, support vector machines, and random forest) to recognize category of a certain POI by spatial and temporal features of human mobility and activity behavior as well as POIs’ locational characteristics. Random forest outperformed the other two methods and obtained an overall accuracy of 72.21%. Apart from that, we compared the results of the different rules in filtering check-in samples. The comparison results show that a reasonable rule to select samples is essential for predicting the category of POI. Moreover, the approach proposed in this study can be potentially applied to identifying functions of buildings according to visitors’ mobility and activity behavior and buildings’ locational characteristics.
Studies of Non-Protective Autophagy Provide Evidence that Recovery from Therapy-Induced Senescence is Independent of Early Autophagy
Authors: Saleh Tareq , Tyutyunyk-Massey Liliya , H. Patel Nipa , K. Cudjoe Emmanuel , Alotaibi Moureq , A. Gewirtz David
Published: February 2020

Autophagy and senescence, predominant responses that may dictate cell fate after chemotherapy or radiation, often occur in tandem. Cells in states of senescence and/or autophagy are frequently growth arrested. We have previously reported that tumor cells induced into senescence by therapy can re-emerge from the growth-arrested state, a phenomenon termed proliferative recovery. The current work shows that, while tumor cells collaterally induced into senescence and autophagy by etoposide, doxorubicin, or radiation undergo proliferative recovery, neither pharmacological nor genetic inhibition of early autophagy alter the extent of senescence or the ability of cells to recover from senescence. These findings confirm and extend our previous observations, essentially dissociating senescence from autophagy, and further indicate that re-emergence from senescence does not appear to be facilitated by or dependent on autophagy. Our results also provide additional evidence for the promotion of the non-protective form of autophagy by both chemotherapeutic drugs and radiation, which may complicate current efforts to inhibit autophagy for therapeutic benefit.
Calcium-Involved Action of Phytochemicals: Carotenoids and Monoterpenes in the Brain
Authors: Rzajew Jowita , Radzik Tomasz , Rebas Elzbieta
Published: February 2020

Background: Neurodegenerative and mood disorders represent growing medical and social problems, many of which are provoked by oxidative stress, disruption in the metabolism of various neurotransmitters, and disturbances in calcium homeostasis. Biologically active plant compounds have been shown to exert a positive impact on the function of calcium in the central nervous system. Methods: The present paper reviews studies of naturally occurring terpenes and derivatives and the calcium-based aspects of their mechanisms of action, as these are known to act upon a number of targets linked to neurological prophylaxis and therapy. Results: Most of the studied phytochemicals possess anticancer, antioxidative, anti-inflammatory, and neuroprotective properties, and these have been used to reduce the risk of or treat neurological diseases. Conclusion: The neuroprotective actions of some phytochemicals may employ mechanisms based on regulation of calcium homeostasis and should be considered as therapeutic agents.
Efficient Prodrug Activator Gene Therapy by Retroviral Replicating Vectors Prolongs Survival in an Immune-Competent Intracerebral Glioma Model
Authors: Chen Shih-Han , Sun Jui-Ming , Chen Bing-Mao , Lin Sheng-Che , Chang Hao-Fang , Collins Sara , Chang Deching , Wu Shu-Fen , Lu Yin-Che , Wang Weijun , Chen Thomas C. , Kasahara Noriyuki , Wang Hsin-Ell , Tai Chien-Kuo
Published: February 2020

Prodrug activator gene therapy mediated by murine leukemia virus (MLV)-based retroviral replicating vectors (RRV) was previously shown to be highly effective in killing glioma cells both in culture and in vivo. To avoid receptor interference and enable dual vector co-infection with MLV-RRV, we have developed another RRV based on gibbon ape leukemia virus (GALV) that also shows robust replicative spread in a wide variety of tumor cells. We evaluated the potential of GALV-based RRV as a cancer therapeutic agent by incorporating yeast cytosine deaminase (CD) and E. coli nitroreductase (NTR) prodrug activator genes into the vector. The expression of CD and NTR genes from GALV-RRV achieved highly efficient delivery of these prodrug activator genes to RG-2 glioma cells, resulting in enhanced cytotoxicity after administering their respective prodrugs 5-fluorocytosine and CB1954 in vitro. In an immune-competent intracerebral RG-2 glioma model, GALV-mediated CD and NTR gene therapy both significantly suppressed tumor growth with CB1954 administration after a single injection of vector supernatant. However, NTR showed greater potency than CD, with control animals receiving GALV-NTR vector alone (i.e., without CB1954 prodrug) showing extensive tumor growth with a median survival time of 17.5 days, while animals receiving GALV-NTR and CB1954 showed significantly prolonged survival with a median survival time of 30 days. In conclusion, GALV-RRV enabled high-efficiency gene transfer and persistent expression of NTR, resulting in efficient cell killing, suppression of tumor growth, and prolonged survival upon CB1954 administration. This validates the use of therapeutic strategies employing this prodrug activator gene to arm GALV-RRV, and opens the door to the possibility of future combination gene therapy with CD-armed MLV-RRV, as the latter vector is currently being evaluated in clinical trials.
NADPH Oxidase Overactivity Underlies Telomere Shortening in Human Atherosclerosis
Authors: Pejenaute Álvaro , Cortés Adriana , Marqués Javier , Montero Laura , Beloqui Óscar , Fortuño Ana , Martí Amelia , Orbe Josune , Zalba Guillermo
Published: February 2020

Telomere shortening and oxidative stress are involved in the pathogenesis of atherosclerosis. Different studies have shown that phagocytic NADPH oxidase is associated with this disease. This study aimed to investigate the association between phagocytic NADPH oxidase and telomere shortening in human atherosclerosis. To assess this potential association, telomere length and phagocytic NADPH oxidase activity were determined by PCR and chemiluminescence, respectively, in a population of asymptomatic subjects free of overt clinical atherosclerosis. We also measured serum 8-hydroxy-2-deoxyguanosine (8-OHdG) levels (an index of oxidative stress) and carotid intima-media thickness (IMT), a surrogate marker of atherosclerosis. After adjusting them for age and sex, telomere length inversely correlated (p < 0.05) with NADPH oxidase-mediated superoxide production, with 8-OHdG values, and with carotid IMT. Interestingly, the asymptomatic subjects with plaques have a lower telomere length (p < 0.05), and higher values of plasma 8-OHdG and superoxide production (p < 0.05). These data were confirmed in a second population in which patients with coronary artery disease showed lower telomere length and higher 8-OHdG and superoxide production than the asymptomatic subjects. In both studies, NADPH oxidase-dependent superoxide production in phagocytic cells was only due to the specific expression of the Nox2 isoform. In conclusion, these findings suggest that phagocytic NADPH oxidase may be involved in oxidative stress-mediated telomere shortening, and that this axis may be critically involved in human atherosclerosis.
Rice OsAAA-ATPase1 is Induced during Blast Infection in a Salicylic Acid-Dependent Manner, and Promotes Blast Fungus Resistance
Authors: Liu Xinqiong , Inoue Haruhiko , Tang Xianying , Tan Yanping , Xu Xin , Wang Chuntai , Jiang Chang-Jie
Published: February 2020

Fatty acids (FAs) have been implicated in signaling roles in plant defense responses. We previously reported that mutation or RNAi-knockdown (OsSSI2-kd) of the rice OsSSI2 gene, encoding a stearoyl acyl carrier protein FA desaturase (SACPD), remarkably enhanced resistance to blast fungus Magnaporthe oryzae and the leaf-blight bacterium Xanthomonas oryzae pv. oryzae (Xoo). Transcriptomic analysis identified six AAA-ATPase family genes (hereafter OsAAA-ATPase1–6) upregulated in the OsSSI2-kd plants, in addition to other well-known defense-related genes. Here, we report the functional analysis of OsAAA-ATPase1 in rice’s defense response to M. oryzae. Recombinant OsAAA-ATPase1 synthesized in Escherichia coli showed ATPase activity. OsAAA-ATPase1 transcription was induced by exogenous treatment with a functional analogue of salicylic acid (SA), benzothiadiazole (BTH), but not by other plant hormones tested. The transcription of OsAAA-ATPase1 was also highly induced in response to M. oryzae infection in an SA-dependent manner, as gene induction was significantly attenuated in a transgenic rice line expressing a bacterial gene (nahG) encoding salicylate hydroxylase. Overexpression of OsAAA-ATPase1 significantly enhanced pathogenesis-related gene expression and the resistance to M. oryzae; conversely, RNAi-mediated suppression of this gene compromised this resistance. These results suggest that OsAAA-APTase1 plays an important role in SA-mediated defense responses against blast fungus M. oryzae.
Functions of Jasmonic Acid in Plant Regulation and Response to Abiotic Stress
Authors: Wang Jia , Song Li , Gong Xue , Xu Jinfan , Li Minhui
Published: February 2020

Jasmonic acid (JA) is an endogenous growth-regulating substance, initially identified as a stress-related hormone in higher plants. Similarly, the exogenous application of JA also has a regulatory effect on plants. Abiotic stress often causes large-scale plant damage. In this review, we focus on the JA signaling pathways in response to abiotic stresses, including cold, drought, salinity, heavy metals, and light. On the other hand, JA does not play an independent regulatory role, but works in a complex signal network with other phytohormone signaling pathways. In this review, we will discuss transcription factors and genes involved in the regulation of the JA signaling pathway in response to abiotic stress. In this process, the JAZ-MYC module plays a central role in the JA signaling pathway through integration of regulatory transcription factors and related genes. Simultaneously, JA has synergistic and antagonistic effects with abscisic acid (ABA), ethylene (ET), salicylic acid (SA), and other plant hormones in the process of resisting environmental stress.
Influence of Diet and Nutrition on Prostate Cancer
Authors: Matsushita Makoto , Fujita Kazutoshi , Nonomura Norio
Published: February 2020

The incidence of prostate cancer (PCa) displays widespread regional differences, probably owing to differences in dietary habits. Nutrients, including fat, protein, carbohydrates, vitamins (vitamin A, D, and E), and polyphenols, potentially affect PCa pathogenesis and progression, as previously reported using animal models; however, clinical studies have reported controversial results for almost all nutrients. The effects of these nutrients may be manifested through various mechanisms including inflammation, antioxidant effects, and the action of sex hormones. Dietary patterns including the Western and Prudent patterns also influence the risk of PCa. Recent studies reported that the gut microbiota contribute to tumorigenesis in some organs. Diet composition and lifestyle have a direct and profound effect on the gut bacteria. Human studies reported an increase in the abundance of specific gut bacteria in PCa patients. Although there are few studies concerning their relationship, diet and nutrition could influence PCa, and this could be mediated by gut microbiota. An intervention of dietary patterns could contribute to the prevention of PCa. An intervention targeting dietary patterns may thus help prevent PCa.
Exposure to Chemotherapy During Childhood or Adulthood and Consequences on Spermatogenesis and Male Fertility
Authors: Delessard Marion , Saulnier Justine , Rives Aurélie , Dumont Ludovic , Rondanino Christine , Rives Nathalie
Published: February 2020

Over the last decade, the number of cancer survivors has increased thanks to progress in diagnosis and treatment. Cancer treatments are often accompanied by adverse side effects depending on the age of the patient, the type of cancer, the treatment regimen, and the doses. The testicular tissue is very sensitive to chemotherapy and radiotherapy. This review will summarize the epidemiological and experimental data concerning the consequences of exposure to chemotherapy during the prepubertal period or adulthood on spermatogenic progression, sperm production, sperm nuclear quality, and the health of the offspring. Studies concerning the gonadotoxicity of anticancer drugs in adult survivors of childhood cancer are still limited compared with those concerning the effects of chemotherapy exposure during adulthood. In humans, it is difficult to evaluate exactly the toxicity of chemotherapeutic agents because cancer treatments often combine chemotherapy and radiotherapy. Thus, it is important to undertake experimental studies in animal models in order to define the mechanism involved in the drug gonadotoxicity and to assess the effects of their administration alone or in combination on immature and mature testis. These data will help to better inform cancer patients after recovery about the risks of chemotherapy for their future fertility and to propose fertility preservation options.
Mechanisms and Therapeutic Regulation of Pyroptosis in Inflammatory Diseases and Cancer
Authors: Zheng Zhaodi , Li Guorong
Published: February 2020

Programmed Cell Death (PCD) is considered to be a pathological form of cell death when mediated by an intracellular program and it balances cell death with survival of normal cells. Pyroptosis, a type of PCD, is induced by the inflammatory caspase cleavage of gasdermin D (GSDMD) and apoptotic caspase cleavage of gasdermin E (GSDME). This review aims to summarize the latest molecular mechanisms about pyroptosis mediated by pore-forming GSDMD and GSDME proteins that permeabilize plasma and mitochondrial membrane activating pyroptosis and apoptosis. We also discuss the potentiality of pyroptosis as a therapeutic target in human diseases. Blockade of pyroptosis by compounds can treat inflammatory disease and pyroptosis activation contributes to cancer therapy.
Homeostasis Imbalance of Microglia and Astrocytes Leads to Alteration in the Metabolites of the Kynurenine Pathway in LPS-Induced Depressive-Like Mice
Authors: Tao Xue , Yan Mingzhu , Wang Lisha , Zhou Yunfeng , Wang Zhi , Xia Tianji , Liu Xinmin , Pan Ruile , Chang Qi
Published: February 2020

In the pathology-oriented study of depression, inflammation hypothesis has received increasing attention for recent years. To mimic the depressive state caused by inflammation, rodents injected intraperitoneally with lipopolysaccharide (LPS) are usually used to stimulate an immune response. However, the dose of LPS that causes depressive-like behavior varies widely across many literatures. Previous study has uncovered the non-linearity in the dose-effect relationship for the depressive-like behavior induced by LPS administration, while the reason for this is still unclear. The present study aims to investigate the underlying mechanisms of this non-linear dose-dependent relationship. Four groups of mice were injected intraperitoneally with different doses of LPS (0, 0.32, 0.8, and 2 mg/kg). The tail suspension test was conducted to evaluate the depressive-like behavior within 23–25 h after the LPS administration. The neuroplasticity was assessed by the levels of related proteins, TrkB and PSD-95, and by the quantification of neurons using Nissl staining. The levels of the two metabolites of the kynurenine (KYN) pathway, 3-hydroxykynurenine (3-HK) and kynurenic acid (KYNA), in the brain were analyzed by LC-MS/MS. Activation of microglia and astrocytes in the brain were also determined by immunohistochemistry and western blotting, respectively. The results showed that, compared with the control group, the mice in the 0.8 mg/kg LPS-treated group exhibited a remarkable increase of immobility time in the tail suspension test. The neuroplasticity of mice in the 0.8 mg/kg LPS-treated group was also significantly reduced. The neurotoxic metabolite, 3-HK, was accumulated significantly in the hippocampus of the 0.8 mg/kg LPS-treated mice. Surprisingly, the 2 mg/kg LPS-treated mice did not exhibit a remarkable change of 3-HK but expressed increased KYNA significantly, which is neuroprotective. Furthermore, the activation of microglia and astrocytes, which were recognized as the primary source of 3-HK and KYNA, respectively, corresponded to the content of these two metabolites of the KYN pathway in each group. Consequently, it was speculated that the homeostasis of different glial cells could lead to a non-linear dose-dependent behavior by regulating the KYN pathway in the LPS-induced depressive-like mice.
WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury
Authors: Chang Chu-Yuan , Liang Min-Zong , Wu Ching-Chih , Huang Pei-Yuan , Chen Hong-I , Yet Shaw-Fang , Tsai Jin-Wu , Kao Cheng-Fu , Chen Linyi
Published: February 2020

The treatment of traumatic brain injury (TBI) remains a challenge due to limited knowledge about the mechanisms underlying neuronal regeneration. This current study compared the expression of WNT genes during regeneration of injured cortical neurons. Recombinant WNT3A showed positive effect in promoting neuronal regeneration via in vitro, ex vivo, and in vivo TBI models. Intranasal administration of WNT3A protein to TBI mice increased the number of NeuN+ neurons without affecting GFAP+ glial cells, compared to control mice, as well as retained motor function based on functional behavior analysis. Our findings demonstrated that WNT3A, 8A, 9B, and 10A promote regeneration of injured cortical neurons. Among these WNTs, WNT3A showed the most promising regenerative potential in vivo, ex vivo, and in vitro.
Interactome and F-Actin Interaction Analysis of Dictyostelium discoideum Coronin A
Authors: Fabrice Tohnyui Ndinyanka , Fiedler Thomas , Studer Vera , Vinet Adrien , Brogna Francesco , Schmidt Alexander , Pieters Jean
Published: February 2020

Coronin proteins are evolutionary conserved WD repeat containing proteins that have been proposed to carry out different functions. In Dictyostelium, the short coronin isoform, coronin A, has been implicated in cytoskeletal reorganization, chemotaxis, phagocytosis and the initiation of multicellular development. Generally thought of as modulators of F-actin, coronin A and its mammalian homologs have also been shown to mediate cellular processes in an F-actin-independent manner. Therefore, it remains unclear whether or not coronin A carries out its functions through its capacity to interact with F-actin. Moreover, the interacting partners of coronin A are not known. Here, we analyzed the interactome of coronin A as well as its interaction with F-actin within cells and in vitro. Interactome analysis showed the association with a diverse set of interaction partners, including fimbrin, talin and myosin subunits, with only a transient interaction with the minor actin10 isoform, but not the major form of actin, actin8, which was consistent with the absence of a coronin A-actin interaction as analyzed by co-sedimentation from cells and lysates. In vitro, however, purified coronin A co-precipitated with rabbit muscle F-actin in a coiled-coil-dependent manner. Our results suggest that an in vitro interaction of coronin A and rabbit muscle actin may not reflect the cellular interaction state of coronin A with actin, and that coronin A interacts with diverse proteins in a time-dependent manner.
Hybridization with Insect Cecropin A (1–8) Improve the Stability and Selectivity of Naturally Occurring Peptides
Authors: Yang Yang , Wu Di , Wang Chenxi , Shan Anshan , Bi Chongpeng , Li Yanbing , Gan Wenping
Published: February 2020

Antimicrobial peptides (AMPs) offer great hope and a promising opportunity to overcome the rapid development of drug-resistant pathogenic microbes. However, AMPs often lack the stability required for a successful systemic drug. Hybridizing different AMPs is a simple and effective strategy to obtain novel peptides. N-terminal fragment of cecropin A (CA (1-8)) is often used to hybridize with other AMPs to reduce cytotoxicity. However, hybridizing with CA (1-8) in improving the stability of AMPs is not clear. Therefore, a series of peptides were designed by combining with CA (1–8) and their antibacterial activity and stability in the presence of salts and human serum were evaluated. The resultant α-helical hybrid peptide CA-FO composed of CA (1-8) and the most potent region of Fowlicidin-2 (FO (1–15)) exhibited excellent antibacterial activity (2-8 μM) and cell selectivity toward bacterial over mammalian cells. Moreover, CA-FO still retained vigorous antimicrobial activity in the presence of human serum and salts at physiological concentrations. CA-FO exhibited effective antibacterial activity by increasing membrane permeability and damaging membrane integrity. In conclusion, these results indicated the success of hybridization in designing and optimizing AMPs with improved stability and selectivity and the peptide CA-FO can be further evaluated as peptide-therapy to treat bacterial infections.
The Role of the RNA-RNA Interactome in the Hepatitis C Virus Life Cycle
Authors: Romero-López Cristina , Berzal-Herranz Alfredo
Published: February 2020

RNA virus genomes are multifunctional entities endowed with conserved structural elements that control translation, replication and encapsidation, among other processes. The preservation of these structural RNA elements constraints the genomic sequence variability. The hepatitis C virus (HCV) genome is a positive, single-stranded RNA molecule with numerous conserved structural elements that manage different steps during the infection cycle. Their function is ensured by the association of protein factors, but also by the establishment of complex, active, long-range RNA-RNA interaction networks-the so-called HCV RNA interactome. This review describes the RNA genome functions mediated via RNA-RNA contacts, and revisits some canonical ideas regarding the role of functional high-order structures during the HCV infective cycle. By outlining the roles of long-range RNA-RNA interactions from translation to virion budding, and the functional domains involved, this work provides an overview of the HCV genome as a dynamic device that manages the course of viral infection.
The Influence of Statins on the Aerobic Metabolism of Endothelial Cells
Authors: Broniarek Izabela , Dominiak Karolina , Galganski Lukasz , Jarmuszkiewicz Wieslawa
Published: February 2020

Endothelial mitochondrial dysfunction is considered to be the main cause of cardiovascular disease. The aim of this research was to elucidate the effects of cholesterol-lowering statins on the aerobic metabolism of endothelial cells at the cellular and mitochondrial levels. In human umbilical vein endothelial cells (EA.hy926), six days of exposure to 100 nM atorvastatin (ATOR) induced a general decrease in mitochondrial respiration. No changes in mitochondrial biogenesis, cell viability, or ATP levels were observed, whereas a decrease in Coenzyme Q10 (Q10) content was accompanied by an increase in intracellular reactive oxygen species (ROS) production, although mitochondrial ROS production remained unchanged. The changes caused by 100 nM pravastatin were smaller than those caused by ATOR. The ATOR-induced changes at the respiratory chain level promoted increased mitochondrial ROS production. In addition to the reduced level of mitochondrial Q10, the activity of Complex III was decreased, and the amount of Complex III in a supercomplex with Complex IV was diminished. These changes may cause the observed decrease in mitochondrial membrane potential and an increase in Q10 reduction level as a consequence, leading to elevated mitochondrial ROS formation. The above observations highlight the role of endothelial mitochondria in response to potential metabolic adaptations related to the chronic exposure of endothelial cells to statins.
Design and Evaluation of an Augmented Reality Game for Cybersecurity Awareness (CybAR)
Authors: Alqahtani Hamed , Kavakli-Thorne Manolya
Published: February 2020

The number of damaging cyberattacks is increasing exponentially due in part to lack of user awareness of risky online practices, such as visiting unsafe websites, ignoring warning messages, and communicating with unauthenticated entities. Although research has established the role that game-based learning can play in cognitive development and conceptual learning, relatively few serious mobile games have been developed to educate users about different forms of cyberattack and ways of avoiding them. This paper reports the development of an effective augmented reality (AR) game designed to increase cybersecurity awareness and knowledge in an active and entertaining way. The Cybersecurity Awareness using Augmented Reality (CybAR) game is an AR mobile application that teaches not only cybersecurity concepts, but also demonstrates the consequences of actual cybersecurity attacks through feedback. The design and evaluation of the application are described in detail. A survey was conducted to verify the effectiveness of the game received positive responses from 91 participants. The results indicate that CybAR is useful for players to develop an understanding of cybersecurity attacks and vulnerabilities.
Comparative Antennal Morphology of Agriotes (Coleoptera: Elateridae), with Special Reference to the Typology and Possible Functions of Sensilla
Authors: Faucheux Michel J. , Németh Tamás , Kundrata Robin
Published: February 2020

Species of the click-beetle genus Agriotes Eschscholtz are economically important crop pests distributed mainly in the Northern Hemisphere. They can inflict considerable damage on various field crops. Therefore, the detection, monitoring, and control of Agriotes include the adult trapping using species-specific sex pheromones, which is a critical component of pest research. To obtain a better understanding of the detailed antennal morphology as background information for subsequent chemical ecology research, we conducted a scanning electron microscopy study of the antennal sensilla of both sexes in 10 European Agriotes species. We identified 16 different sensilla in Agriotes, belonging to six main types: sensilla chaetica (subtypes C1 and C2), sensilla trichodea, sensilla basiconica (subtypes B1–B9), dome-shaped sensilla (subtypes D1 and D2), sensilla campaniformia, and Böhm sensilla. We discuss their possible functions and compare the sensilla of Agriotes with those of other Elateridae in order to consolidate the sensillum nomenclature in this family. Additionally, our study reveals the remarkable interspecific variability in sensillar equipment of Agriotes and identifies several characters of potential importance for future use in systematic studies. The present study provides a strong preliminary framework for subsequent research on the antennal morphology of this crop pest on a wider scale.
Towards a Low-Cost Precision Viticulture System Using Internet of Things Devices
Authors: Spachos Petros
Published: February 2020

Precision Agriculture (PA) is an ever-expanding field that takes modern technological advancements and applies it to farming practices to reduce waste and increase output. One advancement that can play a significant role in achieving precision agriculture is wireless technology, and specifically the Internet of Things (IoT) devices. Small, inch scale and low-cost devices can be used to monitor great agricultural areas. In this paper, a system for precision viticulture which uses IoT devices for real-time monitoring is proposed. The different components of the system are programmed properly and the interconnection between them is designed to minimize energy consumption. Wireless sensor nodes measure soil moisture and soil temperature in the field and transmit the information to a base station. If the conditions are optimal for a disease or pest to occur, a drone flies towards the area. When the drone is over the node, pictures are captured and then it returns to the base station for further processing. The feasibility of the system is examined through experimentation in a realistic scenario.
Unveiling the Secrets of Escher’s Lithographs
Authors: Coltelli Primo , Barsanti Laura , Gualtieri Paolo
Published: February 2020

An impossible structure gives us the impression of looking at a three-dimensional object, even though this object cannot exist, since it possesses parts that are spatially non-connectable, and are characterized by misleading geometrical properties not instantly evident. Therefore, impossible artworks appeal to our intellect and challenge our perceptive capacities. We analyzed lithographs containing impossible structures (e.g., the Necker cube), created by the famous Dutch painter Maurits Cornelis Escher (1898–1972), and used one of them (The Belvedere, 1958) to unveil the artist’s hidden secrets by means of a discrete model of the human retina based on a non-uniform distribution of receptive fields. We demonstrated that the ability of Escher in composing his lithographs by connecting spatial coherent details into an impossible whole lies in drawing these incoherent fragments just outside the zone in which 3D coherence can be perceived during a single fixation pause. The main aspects of our paper from the point of view of image processing and image understanding are the following: (1) the peculiar and original digital filter to process the image, which simulates the human vision process, by producing a space-variant sampling of the image; (2) the software for the filter, which is homemade and created for our purposes. The filtered images resulting from the processing are used to understand impossible figures. As an example, we demonstrate how the impossible figures hidden in Escher’s paintings can be understood.
Best Practices for Shale Core Handling: Transportation, Sampling and Storage for Conduction of Analyses
Authors: Basu Sudeshna , Jones Adrian , Mahzari Pedram
Published: February 2020

Drill core shale samples are critical for palaeoenvironmental studies and potential hydrocarbon reservoirs. They need to be preserved carefully to maximise their retention of reservoir condition properties. However, they are susceptible to alteration due to cooling and depressurisation during retrieval to the surface, resulting in volume expansion and formation of desiccation and micro fractures. This leads to inconsistent measurements of different critical attributes, such as porosity and permeability. Best practices for core handling start during retrieval while extracting from the barrel, followed by correct procedures for transportation and storage. Appropriate preservation measures should be adopted depending on the objectives of the scientific investigation and core coherency, with respect to consolidation and weathering. It is particularly desirable to maintain a constant temperature of 1 to 4 °C and a consistent relative humidity of >75% to minimise any micro fracturing and internal moisture movement in the core. While core re-sampling, it should be ensured that there is no further core compaction, especially while using a hand corer.
Assessing Different Flood Risk and Damage Approaches: A Case of Study in Progreso, Yucatan, Mexico
Authors: Rey Wilmer , Martínez-Amador Miranda , Salles Paulo , Mendoza E. Tonatiuh , Trejo-Rangel Miguel A. , Franklin Gemma L. , Ruiz-Salcines Pablo , Appendini Christian M. , Quintero-Ibáñez Julián
Published: February 2020

This study applies three different methods to assess the flood risk and damage from the strongest high-pressure cold front (locally known as ‘Norte’) event in terms of the residual tide from 30 years (1979–2008) of data for Progreso, Yucatan. The most important difference between the three methods is the estimation of flood vulnerability for Progreso. The first method, proposed by Mexico’s National Center for the Prevention of Disasters (CENAPRED) and used by the Mexican government is based mostly on economic asset (household goods) values and flood impacts. The second (CENAPREDv2) and third (FRI) methods are proposals for assessing risk that include 17 socioeconomic indicators. The former includes economic asset values, as is the case for CENAPRED, while the latter does not. The main results of this study show that the modeled ‘Norte’ event flooded 25% of Progreso’s city blocks, with an estimated economic flood risk of $USD 16,266 (CENAPRED) and $USD 223,779 (CENAPREDv2), and flood damage of $USD 48,848 and $USD 671,918, respectively. When calculating flood risk (FRI) and flood damage (FRI_FD) without monetary terms, the risk categories along the back-barrier behind Progreso varied spatially from ‘very low’ to ‘high’, while areas along the coastal side presented a ‘low’ and ‘very low’ risk. These categories increased for the flood damage because the exceedance probability of the flood was not considered as it was for flood risk in the three methodologies. Therefore, flood damage provides the losses caused by a given flood event without considering how probable that loss may be. In conclusion, this study proposes that the selection of the applied method depends on the main objectives and specific interests when assessing flood risk. For instance, if economic damage is the main concern, then the CENAPRED method should be used as it identifies where the larger economic impacts could occur; when a socioeconomic approach is needed then the FRI should be applied, but if both economic damage and socioeconomic aspects are needed, the CENAPREDv2 is recommended. Besides considering economic aspects, the FRI method also includes social variables that can help to map the most vulnerable population in terms of mobility, education, communication access and others. Therefore, the proposed FRI method is very relevant for disaster risk managers and other stakeholders interested in disaster risk reduction.