bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on Diesel Low-Nitrogen or Nitrogen-Free Combustion Performance in Constant Volume Combustion Vessels and Contributory
Authors: Tan Qinming , Hu Yihuai , Tan Zhiwen
Published: July 2021

This paper studies the combustion performance of diesel in constant volume combustion vessels under different conditions of mixed low-nitrogen (O2 and N2) or non-nitrogen (O2 and CO2) in varying proportions. The high-speed camera is used to shoot the combustion flame in the constant volume combustion vessel. The process and morphology of the combustion flame are amplified in both time and space to study and analyze the effects of different compositions and concentrations in gases on the combustion performance of diesel and conduct a study on the contributory factors in the performance of diesel with no nitrogen. According to the study, in the condition of low nitrogen, the O2 concentration is more than 60%, the ignition delay period is shortened, the combustion flame is bright and slender, it spreads quickly, and the blue flame appears when the O2 concentration reaches 70%; While for nitrogen-free combustion, only when the O2 concentration reaches 30% is the combustion close to the air condition; when the O2 concentration reaches 40%, the combustion condition is optimized obviously and the combustion flame is relatively slender compared to the air working condition. Similarly, with the increase of the O2 concentration, the ignition delay period of nitrogen-free diesel is shortened, the duration is extended, and the combustion performance is optimized. In addition, when the O2 concentration reaches 50%, with the decrease of the initial temperature, the ignition delay period is prolonged, and the duration is shortened obviously. When the temperature is lower than 700 K, there is no ignition. The increase of the diesel injection pressure is beneficial to optimize the ignition performance of diesel non-nitrogen combustion and shorten its ignition delay period and combustion duration. Related research has important guiding significance to optimize nitrogen-free combustion technology, which produces no NOx of the diesel engine.
Analysis and Investigation of Thermal Runaway Propagation for a Mechanically Constrained Lithium-Ion Pouch Cell Module
Authors: Aiello Luigi , Hanzu Ilie , Gstrein Gregor , Ewert Eduard , Ellersdorfer Christian , Sinz Wolfgang
Published: July 2021

In this paper, tests and analysis of thermal runaway propagation for commercial modules consisting of four 41 Ah Li-ion pouch cells are presented. Module samples were tested at 100% state-of-charge and mechanically constrained between two steel plates to provide thermal and mechanical contact between the parts. Voltage and temperature of each cell were monitored during the whole experiment. The triggering of the exothermal reactions was obtained by overheating one cell of the stack with a flat steel heater. In preliminary studies, the melting temperature of the separator was measured (from an extracted sample) with differential scanning calorimetry and thermogravimetric analysis techniques, revealing a tri-layers separator with two melting points (≈135 °C and ≈170 °C). The tests on module level revealed 8 distinct phases observed and analyzed in the respective temperature ranges, including smoking, venting, sparkling, and massive, short circuit condition. The triggering temperature of the cells resulted to be close to the melting temperature of the separator obtained in preliminary tests, confirming that the violent exothermal reactions of thermal runaway are caused by the internal separator failure. Postmortem inspections of the modules revealed the internal electrical failure path in one cell and the propagation of the internal short circuit in its active material volume, suggesting that the expansion of the electrolyte plays a role in the short circuit propagation at the single cell level. The complete thermal runaway propagation process was repeated on 5 modules and ended on average 60 s after the first thermal runaway triggered cell reached a top temperature of 1100 °C.
Towards Accelerated Autolysis? Dynamics of Phenolics, Proteins, Amino Acids and Lipids in Response to Novel Treatments and during Ageing of Sparkling Wine
Authors: Gnoinski Gail B. , Close Dugald C. , Schmidt Simon A. , Kerslake Fiona L.
Published: July 2021

Premium sparkling wine produced by the traditional method (analogous to the French méthode champenoise) is characterised by the development of aged wine character as a result of a second fermentation in the bottle with lees contact and lengthy ageing. Treatments (microwave, ultrasound, or β-glucanase enzymes) were applied to disrupt the cell wall of Saccharomyces cerevisiae and added to the tirage liquor for the second fermentation of Chardonnay-Pinot Noir base wine cuvée and compared to a control, to assess effects on the release of phenolics, proteins, amino acids, and lipids at 6, 12 and 18 months post-tirage. General responses to wine ageing included a 60% increase in the total phenolic content of older sparkling wines relative to younger wines and an increase in protein concentration from 6 to 12 months bottle age. Microwave and β-glucanase enzyme treatments of yeast during tirage preparation were associated with a 10% increase in total free amino acid concentration and a 10% increase in proline concentration at 18 months bottle age, compared to control and ultrasound treatment. Furthermore, microwave treatment was associated with elevated asparagine content in wine at 18 months bottle age, relative to the control and the other wines. The β-glucanase enzyme and ultrasound treatments were associated with significant accumulation of total lipids, which were driven by 2-fold increases in the phospholipid and monoacylglycerol components in wine at 18 months bottle age and, furthermore, the microwave treatment was associated with elevated triacylglycerol at 18 months bottle age. This study demonstrates that the use of yeast treatments at the tirage stage of sparkling wine production presents an opportunity to manipulate wine composition.
De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate Model
Authors: Gonzalez Brittany A. , Perez Gonzalez Marcos , Scholl Frank , Bibevski Steven , Ladich Elena , Bibevski Jennifer , Morales Pablo , Lopez Jesus , Casares Mike , Brehier Vincent , Hernandez Lazaro , Ramaswamy Sharan
Published: July 2021

The utility of implanting a bioscaffold mitral valve consisting of porcine small intestinal submucosa (PSIS) in a juvenile baboon model (12 to 14 months old at the time of implant; n = 3) to assess their in vivo tissue remodeling responses was investigated. Our findings demonstrated that the PSIS mitral valve exhibited the robust presence of de novo extracellular matrix (ECM) at all explantation time points (at 3-, 11-, and 20-months). Apart from a significantly lower level of proteoglycans in the implanted valve’s annulus region (p < 0.05) at 3 months compared to the 11- and 20-month explants, there were no other significant differences (p > 0.05) found between any of the other principal valve ECM components (collagen and elastin) at the leaflet, annulus, or chordae tendinea locations, across these time points. In particular, neochordae tissue had formed, which seamlessly integrated with the native papillary muscles. However, additional processing will be required to trigger accelerated, uniform and complete valve ECM formation in the recipient. Regardless of the specific processing done to the bioscaffold valve, in this proof-of-concept study, we estimate that a 3-month window following bioscaffold valve replacement is the timeline in which complete regeneration of the valve and integration with the host needs to occur.
Efficacy of Immunohistochemistry for SDHB in the Screening of Hereditary Pheochromocytoma–Paraganglioma
Authors: Choi Hye-Ryeon , Koo Ja-Seung , Lee Cho-Rok , Lee Jan-Dee , Kang Sang-Wook , Jo Young-Seok , Chung Woong-Youn
Published: July 2021

The most common genetic backgrounds of hereditary paraganglioma and pheochromocytoma (PPGL) are SDHx germline mutations. Given the fact that the immunohistochemistry (IHC) result for SDHB is always negative regardless of the type of SDHx mutation, we aimed to evaluate the efficacy of using SDHB IHC for screening SDHx mutations in PPGL cases. In total, 52 patients who underwent surgery for PPGL treatment between 2006 and 2020 and underwent genetic analysis at diagnosis were included. Tissue microarrays (TMAs) were constructed with PPGL tissues and IHC for SDHB was performed on TMA sections. All 10 patients with SDHB-negative IHC contained SDHB or SDHD mutations. The genetic test results of patients with SDHB-weakly positive IHC varied (one SDHB, two RET, one VHL, and three unknown gene mutations). There were no SDHx mutations in the SDHB-positive IHC group. Six patients with weakly positive SDHB IHC with primarily unknown genetic status were re-called and underwent next-generation sequencing. None of them had SDHx mutations. In conclusion, SDHB-negative IHC is a cost-effective and reliable method to predict SDHx mutations. However, in the case of weakly positive SDHB staining, an additional gene study should be considered.
Origin and Potential Expansion of the Invasive Longan Lanternfly, Pyrops candelaria (Hemiptera: Fulgoridae) in Taiwan
Authors: Lin You-Sheng , Liao Jhih-Rong , Shiao Shiuh-Feng , Ko Chiun-Cheng
Published: July 2021

The longan lanternfly Pyrops candelaria is a new invasive species on the main island of Taiwan. The introduction of an invasive species may negatively influence the native fauna, flora and environment. Thus, this study aimed to infer the invasion history, predict habitat suitability and potential expansion and assess the risk to crop cultivation areas in Taiwan. Genetic structures of P. candelaria from the main island of Taiwan and related regions were analyzed based on partial COI and ND2 sequences. Additionally, machine learning MaxEnt was utilized to study habitat suitability. The results suggested that the Taiwanese populations may originate from the Kinmen Islands and the plain areas of Taiwan are considered to have high habitat suitability. Furthermore, most of the cultivation areas of longan and pomelo crops showed high habitat suitability.
SWATH-MS Quantitative Proteomic Analysis of Deer Antler from Two Regenerating and Mineralizing Sections
Authors: López-Pedrouso María , Lorenzo José , Landete-Castillejos Tomás , Chonco Louis , Pérez-Barbería Francisco , García Andrés , López-Garrido María-Pilar , Franco Daniel
Published: July 2021

Antlers are the only organ in the mammalian body that regenerates each year. They can reach growth rates of 1–3 cm/day in length and create more than 20 cm2/day of skin in the antler tips (their growth centers). Previous proteomic studies regarding antlers have focused on antler growth centers (tips) compared to the standard bone to detect the proteins involved in tissue growth. However, proteins of cell differentiation and regeneration will be more accurately detected considering more growing tissues. Thus, we set out to compare proteins expressed in antler tips (the highest metabolism rate and cell differentiation) vs. middle sections (moderate cell growth involving bone calcification), using ribs as controls. Samples were obtained in mid-June with antlers’ phenology corresponding to the middle of their growth period. Quantitative proteomic analysis identified 259 differentially abundant proteins mainly associated with antioxidant metabolic mechanisms, protein formation and Wnt signalling pathway, meanwhile, the mid antler section was linked to blood proteins. The high metabolic rate and subsequent risk of oxidative stress also seem to have resulted in strong antioxidant mechanisms. These results suggest that redox regulation of proteins is a key factor in the model of deer antler regeneration.
The Role of Monk Parakeets as Nest-Site Facilitators in Their Native and Invaded Areas
Authors: Hernández-Brito Dailos , Carrete Martina , Blanco Guillermo , Romero-Vidal Pedro , Senar Juan Carlos , Mori Emiliano , White Thomas H. , Luna Álvaro , Tella José L.
Published: July 2021

While most of the knowledge on invasive species focuses on their impacts, little is known about their potential positive effects on other species. Invasive ecosystem engineers can disrupt recipient environments; however, they may also facilitate access to novel resources for native species. The monk parakeet (Myiopsitta monachus) is a worldwide invader and the only parrot that builds its own communal nests, which can be used by other species. However, the ecological effects of these interspecific interactions are barely known. We compared the role of the monk parakeet as a nest-site facilitator in different rural and urban areas, both invaded and native, across three continents and eight breeding seasons. A total of 2690 nests from 42 tenant species, mostly cavity-nesting birds, were recorded in 26% of 2595 monk parakeet nests. Rural and invaded areas showed the highest abundance and richness of tenant species. Multispecies communal nests triggered interspecific aggression between the monk parakeet host and its tenants, but also a cooperative defense against predators. Despite the positive effects for native species, monk parakeets also facilitate nesting opportunities to other non-native species and may also transmit diseases to tenants, highlighting the complexity of biotic interactions in biological invasions.
Celecoxib Analogues for Cancer Treatment: An Update on OSU-03012 and 2,5-Dimethyl-Celecoxib
Authors: Sobolewski Cyril , Legrand Noémie
Published: July 2021

Cyclooxygenase-2 (COX-2) is an important enzyme involved in prostaglandins biosynthesis from arachidonic acid. COX-2 is frequently overexpressed in human cancers and plays a major tumor promoting function. Accordingly, many efforts have been devoted to efficiently target the catalytic site of this enzyme in cancer cells, by using COX-2 specific inhibitors such as celecoxib. However, despite their potent anti-tumor properties, the myriad of detrimental effects associated to the chronic inhibition of COX-2 in healthy tissues, has considerably limited their use in clinic. In addition, increasing evidence indicate that these anti-cancerous properties are not strictly dependent on the inhibition of the catalytic site. These findings have led to the development of non-active COX-2 inhibitors analogues aiming at preserving the antitumor effects of COX-2 inhibitors without their side effects. Among them, two celecoxib derivatives, 2,5-Dimethyl-Celecoxib and OSU-03012, have been developed and suggested for the treatment of viral (e.g., recently SARS-CoV-2), inflammatory, metabolic diseases and cancers. These molecules display stronger anti-tumor properties than celecoxib and thus may represent promising anti-cancer molecules. In this review, we discuss the impact of these two analogues on cancerous processes but also their potential for cancer treatment alone or in combination with existing approaches.
Sesquiterpene Lactones with the 12,8-Guaianolide Skeleton from Algerian Centaurea omphalotricha
Authors: Kebbi Sara , Ciavatta Maria Letizia , Mahmoud Ali Mokhtar , Carbone Marianna , Ligresti Alessia , Seghiri Ramdane , Gavagnin Margherita
Published: July 2021

In continuing our investigation on the chemical diversity of Algerian plants, we examined Centaurea omphalotricha, whose chemical composition has been poorly studied. The present work was aimed at characterizing the secondary metabolite pattern of the CHCl3 extract of the aerial parts of this plant that displayed antiproliferative properties in a preliminary screening on HeLa cell line. The chemical analysis led us to characterize the bioactive oxygenated terpenoid fraction which includes, within major known metabolites, two new minor sesquiterpene lactones, centaurolide-A (1) and centaurolide-B (2). The structures of two compounds exhibiting the 12,8-guaianolide skeleton were determined by spectroscopic methods as well as by chemical correlation with inuviscolide (3), a well-known bioactive guaianolide isolated from Dittrichia (=Inula) viscosa. Centaurolides A and B represent the first report of 8,12-guaianolide skeleton in Centaurea genus. The effect of new compounds 1 and 2 and inuviscolide (3) on HeLa cell has also been evaluated.
Wrestling and Wrapping: A Perspective on SUMO Proteins in Schwann Cells
Authors: Fergani Iman F. , Frick Luciana R.
Published: July 2021

Schwann cell development and peripheral nerve myelination are finely orchestrated multistep processes; some of the underlying mechanisms are well described and others remain unknown. Many posttranslational modifications (PTMs) like phosphorylation and ubiquitination have been reported to play a role during the normal development of the peripheral nervous system (PNS) and in demyelinating neuropathies. However, a relatively novel PTM, SUMOylation, has not been studied in these contexts. SUMOylation involves the covalent attachment of one or more small ubiquitin-like modifier (SUMO) proteins to a substrate, which affects the function, cellular localization, and further PTMs of the conjugated protein. SUMOylation also regulates other proteins indirectly by facilitating non-covalent protein–protein interaction via SUMO interaction motifs (SIM). This pathway has important consequences on diverse cellular processes, and dysregulation of this pathway has been reported in several diseases including neurological and degenerative conditions. In this article, we revise the scarce literature on SUMOylation in Schwann cells and the PNS, we propose putative substrate proteins, and we speculate on potential mechanisms underlying the possible involvement of this PTM in peripheral myelination and neuropathies.
Glucose-Dependent Insulinotropic Polypeptide Suppresses Foam Cell Formation of Macrophages through Inhibition of the Cyclin-Dependent Kinase 5-CD36 Pathway
Authors: Terasaki Michishige , Yashima Hironori , Mori Yusaku , Saito Tomomi , Shiraga Yoshie , Kawakami Raichi , Ohara Makoto , Fukui Tomoyasu , Hirano Tsutomu , Yamada Yuichiro , Seino Yutaka , Yamagishi Sho-ichi
Published: July 2021

Glucose-dependent insulinotropic polypeptide (GIP) has been reported to have an atheroprotective property in animal models. However, the effect of GIP on macrophage foam cell formation, a crucial step of atherosclerosis, remains largely unknown. We investigated the effects of GIP on foam cell formation of, and CD36 expression in, macrophages extracted from GIP receptor-deficient (Gipr−/−) and Gipr+/+ mice and cultured human U937 macrophages by using an agonist for GIP receptor, [D-Ala2]GIP(1–42). Foam cell formation evaluated by esterification of free cholesterol to cholesteryl ester and CD36 gene expression in macrophages isolated from Gipr+/+ mice infused subcutaneously with [D-Ala2]GIP(1–42) were significantly suppressed compared with vehicle-treated mice, while these beneficial effects were not observed in macrophages isolated from Gipr−/− mice infused with [D-Ala2]GIP(1–42). When macrophages were isolated from Gipr+/+ and Gipr−/− mice, and then exposed to [D-Ala2]GIP(1–42), similar results were obtained. [D-Ala2]GIP(1–42) attenuated ox-LDL uptake of, and CD36 gene expression in, human U937 macrophages as well. Gene expression level of cyclin-dependent kinase 5 (Cdk5) was also suppressed by [D-Ala2]GIP(1–42) in U937 cells, which was corelated with that of CD36. A selective inhibitor of Cdk5, (R)-DRF053 mimicked the effects of [D-Ala2]GIP(1–42) in U937 cells. The present study suggests that GIP could inhibit foam cell formation of macrophages by suppressing the Cdk5-CD36 pathway via GIP receptor.
Role of PITRM1 in Mitochondrial Dysfunction and Neurodegeneration
Authors: Brunetti Dario , Catania Alessia , Viscomi Carlo , Deleidi Michela , Bindoff Laurence A. , Ghezzi Daniele , Zeviani Massimo
Published: July 2021

Mounting evidence shows a link between mitochondrial dysfunction and neurodegenerative disorders, including Alzheimer Disease. Increased oxidative stress, defective mitodynamics, and impaired oxidative phosphorylation leading to decreased ATP production, can determine synaptic dysfunction, apoptosis, and neurodegeneration. Furthermore, mitochondrial proteostasis and the protease-mediated quality control system, carrying out degradation of potentially toxic peptides and misfolded or damaged proteins inside mitochondria, are emerging as potential pathogenetic mechanisms. The enzyme pitrilysin metallopeptidase 1 (PITRM1) is a key player in these processes; it is responsible for degrading mitochondrial targeting sequences that are cleaved off from the imported precursor proteins and for digesting a mitochondrial fraction of amyloid beta (Aβ). In this review, we present current evidence obtained from patients with PITRM1 mutations, as well as the different cellular and animal models of PITRM1 deficiency, which points toward PITRM1 as a possible driving factor of several neurodegenerative conditions. Finally, we point out the prospect of new diagnostic and therapeutic approaches.
Alginate-Agarose Hydrogels Improve the In Vitro Differentiation of Human Dental Pulp Stem Cells in Chondrocytes. A Histological Study
Authors: Oliver-Ferrándiz María , Milián Lara , Sancho-Tello María , Martín de Llano José Javier , Gisbert Roca Fernando , Martínez-Ramos Cristina , Carda Carmen , Mata Manuel
Published: July 2021

Matrix-assisted autologous chondrocyte implantation (MACI) has shown promising results for cartilage repair, combining cultured chondrocytes and hydrogels, including alginate. The ability of chondrocytes for MACI is limited by different factors including donor site morbidity, dedifferentiation, limited lifespan or poor proliferation in vitro. Mesenchymal stem cells could represent an alternative for cartilage regeneration. In this study, we propose a MACI scaffold consisting of a mixed alginate-agarose hydrogel in combination with human dental pulp stem cells (hDPSCs), suitable for cartilage regeneration. Scaffolds were characterized according to their rheological properties, and their histomorphometric and molecular biology results. Agarose significantly improved the biomechanical behavior of the alginate scaffolds. Large scaffolds were manufactured, and a homogeneous distribution of cells was observed within them. Although primary chondrocytes showed a greater capacity for chondrogenic differentiation, hDPSCs cultured in the scaffolds formed large aggregates of cells, acquired a rounded morphology and expressed high amounts of type II collagen and aggrecan. Cells cultured in the scaffolds expressed not only chondral matrix-related genes, but also remodeling proteins and chondrocyte differentiation factors. The degree of differentiation of cells was proportional to the number and size of the cell aggregates that were formed in the hydrogels.
High-Density Lipoprotein Subfractions: Much Ado about Nothing or Clinically Important?
Authors: Lappegård Knut Tore , Kjellmo Christian Abendstein , Hovland Anders
Published: July 2021

High-density lipoproteins (HDL) are a heterogenous group of plasma molecules with a large variety in composition. There is a wide specter in lipid content and the number of different proteins that has been associated with HDL is approaching 100. Given this heterogeneity and the fact that the total amount of HDL is inversely related to the risk of coronary heart disease (CHD), there has been increasing interest in the function of specific HDL subgroups and in what way measuring and quantifying these subgroups could be of clinical importance in determining individual CHD risk. If certain subgroups appear to be more protective than others, it may also in the future be possible to pharmacologically increase beneficial and decrease harmful subgroups in order to reduce CHD risk. In this review we give a short historical perspective, summarize some of the recent clinical findings regarding HDL subclassifications and discuss why such classification may or may not be of clinical relevance.
Pitavastatin Ameliorates Lipopolysaccharide-Induced Blood-Brain Barrier Dysfunction
Authors: Fujimoto Takashi , Morofuji Yoichi , Kovac Andrej , Erickson Michelle A. , Deli Mária A. , Niwa Masami , Banks William A.
Published: July 2021

Statins have neuroprotective effects on neurological diseases, including a pleiotropic effect possibly related to blood–brain barrier (BBB) function. In this study, we investigated the effects of pitavastatin (PTV) on lipopolysaccharide (LPS)-induced BBB dysfunction in an in vitro BBB model comprising cocultured primary mouse brain endothelial cells, pericytes, and astrocytes. LPS (1 ng/mL, 24 h) increased the permeability and lowered the transendothelial electrical resistance of the BBB, and the co-administration of PTV prevented these effects. LPS increased the release of interleukin-6, granulocyte colony-stimulating factor, keratinocyte-derived chemokine, monocyte chemotactic protein-1, and regulated on activation, normal T-cell expressed and secreted from the BBB model. PTV inhibited the LPS-induced release of these cytokines. These results suggest that PTV can ameliorate LPS-induced BBB dysfunction, and these effects might be mediated through the inhibition of LPS-induced cytokine production. Clinically, therapeutic approaches using statins combined with novel strategies need to be designed. Our present finding sheds light on the pharmacological significance of statins in the treatment of central nervous system diseases.
Biochemical Biomarkers and Neurodegenerative Diseases
Authors: Ciaccio Marcello
Published: July 2021

Neurodegenerative diseases (ND) are a heterogeneous group of disorders characterized by progressive dysfunction and loss of neurons in different areas of the central nervous system or peripheral nervous system [...]
Contemporary Odeon Buildings as a Sustainable Environment for Culture
Authors: Butelski Kazimierz Leonard
Published: July 2021

The subject of this study is contemporary odeons in Poland, where 11 covered amphitheaters (odeons) have been built since 2005. The odeons were selected from a wider collection of 57 functioning amphitheaters. The study collected data on location, form, function, and construction. The data sources included the literature, archival research, design documentation, and competition entries. Descriptive and graphical comparative analyses of the phenomena, based on the statistics for completed structures and on design experiments in the case of unbuilt structures, were the two main research methods used in this study. The emergence and development directions of the typology of open cultural spaces from amphitheaters to odeons are presented in a global and regional context. Their interrelationships, affecting form and function, were also analyzed. The influence of high-end materials that were used to create these complex, large-scale spatial structures, and their impact on the environment, has been presented. The contemporary roofs covering the entertainment and stage complex were analyzed in relation to environmental factors, determining the location of the odeons. The functional aspects of these buildings and their cultural significance on a local, regional and global scale were discussed. The odeon in Biała Podlaska, built in 2019, was chosen as a case study to show, in detail, the complexity of the formation of contemporary odeons. In the discussion on the direction of the further evolution of open spaces for culture, an example of an unrealized competition design proposal of mobile roofing forms for the eighteenth-century amphitheater in the Royal Baths Park in Warsaw, Poland, was presented. The conclusions emphasize the environmental, spatial, functional, social and economic values of the establishment and functioning of contemporary odeons as open spaces of culture that are compliant with the principles of sustainable development.
Application of Advanced Building Techniques to Enhance the Environmental Performance of Interior Components
Authors: Celadyn Magdalena , Celadyn Waclaw
Published: July 2021

This paper discusses the impact of advanced building techniques, in tune with selected building materials and their physical attributes, applied to complete constitutive interiors components on these components’ environmental performance and aesthetics. There is an understanding of technological practices as essential for the effective management of the design process; still, the creative introduction of advanced building techniques is not commonly recognized by interior architects. The objective of the research is to indicate the possible multidimensional consequences of the analysis of materials’ physical attributes and the consistent application of advanced building techniques to complete interior components. The basis for this study formed the design concepts of aesthetic functionalism, place attachment, and a content-context model of the association between interior components and the building fabric. Some theoretical frameworks were used for a qualitative evaluation of interior components of selected cultural facilities completed in the last decade in Poland. The performance of these components was measured in the function of applied innovative building techniques and specified building materials. Research findings have proved the impact of building techniques on the performance of interior components as instruments to increase interior functional use, formal uniformity, and aesthetic cohesion of buildings and their inner spaces, as well as the scale of multisensorial effectiveness.
Fused Filament Fabrication Three-Dimensional Printing Multi-Functional of Polylactic Acid/Carbon Black Nanocomposites
Authors: Vidakis Nectarios , Petousis Markos , Velidakis Emmanuel , Mountakis Nikolaos , Fischer-Griffiths Peder Erik , Grammatikos Sotirios , Tzounis Lazaros
Published: July 2021

Conductive Polymer Composites (CPCs) have recently gained an extensive scientific interest as feedstock materials in Fused Filament Fabrication (FFF) Three-dimensional (3D) printing. Polylactic Acid (PLA), widely used in FFF 3D printing, as well as its Carbon Black (CB) nanocomposites at different weight percentage (wt.%) filler loadings (0.5, 1.0, 2.5 and 5.0 wt.%), were prepared via a melt mixing filament extrusion process in this study and utilized to manufacture FFF 3D printed specimens. The nanocomposites were examined for their electrical conductivity. The highest loaded 3D printed CPC (5.0 wt.%) was tested as an electrothermal Joule heating device. Static tensile, flexural, Charpy’s impact and Vickers microhardness mechanical properties were investigated for the neat and PLA/CB 3D printed nanocomposites. Dynamic Mechanical Analysis (DMA) revealed a stiffening mechanism for the PLA/CB nanocomposites. Scanning Electron Microscopy (SEM) elucidated the samples’ internal and external microstructural characteristics. The PLA/CB 5.0 wt.% nanocomposite demonstrated also antibacterial properties, when examined with a screening process, against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). It can be envisaged that the 3D printed PLA/CB CPCs exhibited a multi-functional performance, and could open new avenues towards low-cost personalized biomedical objects with complex geometry, amongst others, i.e., surgery tools, splints, wearables, etc.
DNA Methylation and Intra-Clonal Heterogeneity: The Chronic Myeloid Leukemia Model
Authors: Lebecque Benjamin , Bourgne Céline , Vidal Véronique , Berger Marc G.
Published: July 2021

Chronic Myeloid Leukemia (CML) is a model to investigate the impact of tumor intra-clonal heterogeneity in personalized medicine. Indeed, tyrosine kinase inhibitors (TKIs) target the BCR-ABL fusion protein, which is considered the major CML driver. TKI use has highlighted the existence of intra-clonal heterogeneity, as indicated by the persistence of a minority subclone for several years despite the presence of the target fusion protein in all cells. Epigenetic modifications could partly explain this heterogeneity. This review summarizes the results of DNA methylation studies in CML. Next-generation sequencing technologies allowed for moving from single-gene to genome-wide analyses showing that methylation abnormalities are much more widespread in CML cells. These data showed that global hypomethylation is associated with hypermethylation of specific sites already at diagnosis in the early phase of CML. The BCR-ABL-independence of some methylation profile alterations and the recent demonstration of the initial intra-clonal DNA methylation heterogeneity suggests that some DNA methylation alterations may be biomarkers of TKI sensitivity/resistance and of disease progression risk. These results also open perspectives for understanding the epigenetic/genetic background of CML predisposition and for developing new therapeutic strategies.
Correlation of Body Mass Index with Oncologic Outcomes in Colorectal Cancer Patients: A Large Population-Based Study
Authors: Chiu Chong-Chi , Ho Chung-Han , Hung Chao-Ming , Chao Chien-Ming , Lai Chih-Cheng , Chen Chin-Ming , Liao Kuang-Ming , Wang Jhi-Joung , Wu Yu-Cih , Shi Hon-Yi , Lee Po-Huang , Lee Hui-Ming , Yeh Li-Ren , Soong Tien-Chou , Chiang Shyh-Ren , Cheng Kuo-Chen
Published: July 2021

It has been acknowledged that excess body weight increases the risk of colorectal cancer (CRC); however, there is little evidence on the impact of body mass index (BMI) on CRC patients’ long-term oncologic results in Asian populations. We studied the influence of BMI on overall survival (OS), disease-free survival (DFS), and CRC-specific survival rates in CRC patients from the administrative claims datasets of Taiwan using the Kaplan–Meier survival curves and the log-rank test to estimate the statistical differences among BMI groups. Underweight patients (<18.50 kg/m2) presented higher mortality (56.40%) and recurrence (5.34%) rates. Besides this, they had worse OS (aHR:1.61; 95% CI: 1.53–1.70; p-value: < 0.0001) and CRC-specific survival (aHR:1.52; 95% CI: 1.43–1.62; p-value: < 0.0001) rates compared with those of normal weight patients (18.50–24.99 kg/m2). On the contrary, CRC patients belonging to the overweight (25.00–29.99 kg/m2), class I obesity (30.00–34.99 kg/m2), and class II obesity (≥35.00 kg/m2) categories had better OS, DFS, and CRC-specific survival rates in the analysis than the patients in the normal weight category. Overweight patients consistently had the lowest mortality rate after a CRC diagnosis. The associations with being underweight may reflect a reverse causation. CRC patients should maintain a long-term healthy body weight.
Assessing Versatile Machine Learning Models for Glioma Radiogenomic Studies across Hospitals
Authors: Kawaguchi Risa K. , Takahashi Masamichi , Miyake Mototaka , Kinoshita Manabu , Takahashi Satoshi , Ichimura Koichi , Hamamoto Ryuji , Narita Yoshitaka , Sese Jun
Published: July 2021

Radiogenomics use non-invasively obtained imaging data, such as magnetic resonance imaging (MRI), to predict critical biomarkers of patients. Developing an accurate machine learning (ML) technique for MRI requires data from hundreds of patients, which cannot be gathered from any single local hospital. Hence, a model universally applicable to multiple cohorts/hospitals is required. We applied various ML and image pre-processing procedures on a glioma dataset from The Cancer Image Archive (TCIA, n = 159). The models that showed a high level of accuracy in predicting glioblastoma or WHO Grade II and III glioma using the TCIA dataset, were then tested for the data from the National Cancer Center Hospital, Japan (NCC, n = 166) whether they could maintain similar levels of high accuracy. Results: we confirmed that our ML procedure achieved a level of accuracy (AUROC = 0.904) comparable to that shown previously by the deep-learning methods using TCIA. However, when we directly applied the model to the NCC dataset, its AUROC dropped to 0.383. Introduction of standardization and dimension reduction procedures before classification without re-training improved the prediction accuracy obtained using NCC (0.804) without a loss in prediction accuracy for the TCIA dataset. Furthermore, we confirmed the same tendency in a model for IDH1/2 mutation prediction with standardization and application of dimension reduction that was also applicable to multiple hospitals. Our results demonstrated that overfitting may occur when an ML method providing the highest accuracy in a small training dataset is used for different heterogeneous data sets, and suggested a promising process for developing an ML method applicable to multiple cohorts.
Chemotherapy-Induced Myopathy: The Dark Side of the Cachexia Sphere
Authors: Campelj Dean G. , Goodman Craig A. , Rybalka Emma
Published: July 2021

Cancer cachexia is a debilitating multi-factorial wasting syndrome characterised by severe skeletal muscle wasting and dysfunction (i.e., myopathy). In the oncology setting, cachexia arises from synergistic insults from both cancer–host interactions and chemotherapy-related toxicity. The majority of studies have surrounded the cancer–host interaction side of cancer cachexia, often overlooking the capability of chemotherapy to induce cachectic myopathy. Accumulating evidence in experimental models of cachexia suggests that some chemotherapeutic agents rapidly induce cachectic myopathy, although the underlying mechanisms responsible vary between agents. Importantly, we highlight the capacity of specific chemotherapeutic agents to induce cachectic myopathy, as not all chemotherapies have been evaluated for cachexia-inducing properties—alone or in clinically compatible regimens. Furthermore, we discuss the experimental evidence surrounding therapeutic strategies that have been evaluated in chemotherapy-induced cachexia models, with particular focus on exercise interventions and adjuvant therapeutic candidates targeted at the mitochondria.
Fabrication of Six Manganese Containing Polyoxometalate Modified Graphite C3N4 Nanosheets Catalysts Used to Catalyze Water Decomposition
Authors: Wu Yue , Yu Xiaoxia , Fu Zhijing , Pei Jianye , Bi Lihua
Published: July 2021

With the increase in gas population, the demand for clean and renewable energy is increasing. Hydrogen energy has a high combustion conversion energy while water is its combustion product. In recent years, a way to convert water into hydrogen and oxygen has been found by human beings inspired by plant photosynthesis. However, water decomposition consumes a significant amount of energy and is expensive. People expect to obtain a water decomposition catalyst with low cost and high efficiency. This work selected a six-manganese containing polyoxometalate with a similar structure characteristic to photosynthesizing PSII to fabricate with graphite C3N4 nanosheets for the construction of composite film (Mn6SiW/g-C3N4NSs) electrode via layer by layer self-assembly technology, which was used for the photo-electrochemical decomposition of water under visible light conditions. The binary composite film electrode displayed good catalytic efficiency. The photoelectric density of the composite electrode is 46 μA/cm2 (at 1.23 V vs. Ag/AgCl) and 239 μA/cm2 (at 1.5 V vs. Ag/AgCl). Compared with the g-C3N4NSs electrode alone, the photoelectric density of the composite electrode increased by 1 time. The reason is attributed to the fact that Mn6SiW has a similar structure characteristic to photosynthesizing PSII and high electron transferability. The construction of the composite film containing low-cost Mn6SiW to modify g-C3N4NSs can effectively improve the photocatalytic decomposition of water, thus this study provides valuable reference information for the development of low-cost and high-performance photo-electrocatalytic materials.
Direct Cross-Coupling of Alcohols with O-Nucleophiles Mediated by N-Iodosuccinimide as a Precatalyst under Mild Reaction Conditions
Authors: Ajvazi Njomza , Stavber Stojan
Published: July 2021

We report N-iodosuccinimide as the most efficient and selective precatalyst among the N-halosuccinimides for dehydrative O-alkylation reactions between various alcohols under high-substrate concentration reaction conditions. The protocol is non-metal, one-pot, selective, and easily scalable, with excellent yields; enhancing the green chemical profiles of these transformations.
Decomposition of Tars on a Nickel Honeycomb Catalyst
Authors: Woroszył-Wojno Joanna , Młotek Michał , Perron Michalina , Jóźwik Paweł , Ulejczyk Bogdan , Krawczyk Krzysztof
Published: July 2021

Biomass can be considered a renewable energy source. It undergoes a gasification process to obtain gaseous fuel, which converts it into combustible gaseous products such as hydrogen, carbon monoxide, and methane. The process also generates undesirable tars that can condense in gas lines and cause corrosion, and after processing, can be an additional source of combustible gases. This study focused on the processing of tar substances with toluene as a model substance. The effect of discharge power and carrier gas composition on toluene conversion was tested. The process was conducted in a plasma-catalytic system with a new Ni3Al system in the form of a honeycomb. The toluene conversion reached 90%, and small amounts of ethane, ethylene, acetylene, benzene, and C3 and C4 hydrocarbons were detected in the post-reaction mixture. Changes in the surface composition of the Ni3Al catalyst were observed throughout the experiments. These changes did not affect the toluene conversion.
A Thermophilic GH5 Endoglucanase from Aspergillus fumigatus and Its Synergistic Hydrolysis of Mannan-Containing Polysaccharides
Authors: Gu Xinxi , Lu Haiqiang , Zhang Lijuan , Meng Xiangchen
Published: July 2021

In this study, we isolated and identified a thermophilic strain of Aspergillus fumigatus from the “Daqu” samples. Transcriptomic analysis of A. fumigatus identified 239 carbohydrate-active enzymes (CAZy)-encoding genes, including 167 glycoside hydrolase (GH)-encoding genes, 58 glycosyltransferase (GT)-encoding genes, 2 polysaccharide lyase (PLs)-encoding genes and 12 carbohydrate esterase (CEs)-encoding genes, which indicates that the strain has a strong potential for application for enzyme production. Furthermore, we also identified a novel endoglucanase gene (AfCel5A), which was expressed in Pichia pastoris and characterized. The novel endoglucanase AfCel5A exhibited the highest hydrolytic activity against CMC-Na and the optimal activity at 80 °C and pH 4.0 and also showed good stability at pH 3.0–11.0 and below 70 °C. The Km and Vmax values of AfCel5 were 0.16 ± 0.05 mg·mL−1 and 7.23 ± 0.33 mol mg−1·min−1, respectively, using CMC-Na as a substrate. Further, the endoglucanase exhibited a high tolerance toward NaCl as well as glucose. In addition, the finding that the endoglucanase AfCel5A in combination with β-mannanse (ManBK) clearly increased the release of total reducing sugars of glucomannan by up to 74% is significant.
A Review on Lactoferrin and Central Nervous System Diseases
Authors: Li Yu-Qi , Guo Chuang
Published: July 2021

Central nervous system (CNS) diseases are currently one of the major health issues around the world. Most CNS disorders are characterized by high oxidative stress levels and intense inflammatory responses in affected tissues. Lactoferrin (Lf), a multifunctional iron-binding glycoprotein, plays a significant role in anti-inflammatory, antibacterial, antiviral, reactive oxygen species (ROS) modulator, antitumor immunity, and anti-apoptotic processes. Previous studies have shown that Lf is abnormally expressed in a variety of neurological diseases, especially neurodegenerative diseases. Recently, the promotion of neurodevelopment and neuroprotection by Lf has attracted widespread attention, and Lf could be exploited both as an active therapeutic agent and drug nanocarrier. However, our understanding of the roles of Lf proteins in the initiation or progression of CNS diseases is limited, especially the roles of Lf in regulating neurogenesis. This review highlights recent advances in the understanding of the major pharmacological effects of Lf in CNS diseases, including neurodegenerative diseases, cerebrovascular disease, developmental delays in children, and brain tumors.
Protective Response in Experimental Paracoccidioidomycosis Elicited by Extracellular Vesicles Containing Antigens of Paracoccidioides brasiliensis
Authors: Baltazar Ludmila Matos , Ribeiro Gabriela Fior , Freitas Gustavo J. , Queiroz-Junior Celso Martins , Fagundes Caio Tavares , Chaves-Olórtegui Carlos , Teixeira Mauro Martins , Souza Daniele G.
Published: July 2021

Paracoccidioidomycosis (PCM) is a systemic disease caused by Paracoccidioides spp. PCM is endemic in Latin America and most cases are registered in Brazil. This mycosis affects mainly the lungs, but can also spread to other tissues and organs, including the liver. Several approaches have been investigated to improve treatment effectiveness and protection against the disease. Extracellular vesicles (EVs) are good antigen delivery vehicles. The present work aims to investigate the use of EVs derived from Paracoccidioides brasiliensis as an immunization tool in a murine model of PCM. For this, male C57BL/6 were immunized with two doses of EVs plus adjuvant and then infected with P. brasiliensis. EV immunization induced IgM and IgG in vivo and cytokine production by splenocytes ex vivo. Further, immunization with EVs had a positive effect on mice infected with P. brasiliensis, as it induced activated T lymphocytes and NKT cell mobilization to the infected lungs, improved production of proinflammatory cytokines and the histopathological profile, and reduced fungal burden. Therefore, the present study shows a new role for P. brasiliensis EVs in the presence of adjuvant as modulators of the host immune system, suggesting their utility as immunizing agents.
Sodium-Glucose Cotransporter-2 Inhibitors in Patients with Hereditary Podocytopathies, Alport Syndrome, and FSGS: A Case Series to Better Plan a Large-Scale Study
Authors: Boeckhaus Jan , Gross Oliver
Published: July 2021

Hereditary diseases of the glomerular filtration barrier are characterized by a more vulnerable glomerular basement membrane and dysfunctional podocytes. Recent clinical trials have demonstrated the nephroprotective effect of sodium-glucose cotransporter-2 inhibitors (SGLT2i) in chronic kidney disease (CKD). SGLT2-mediated afferent arteriole vasoconstriction is hypothesized to correct the hemodynamic overload of the glomerular filtration barrier in hereditary podocytopathies. To test this hypothesis, we report data in a case series of patients with Alport syndrome and focal segmental glomerulosclerosis (FSGS) with respect of the early effect of SGLT2i on the kidney function. Mean duration of treatment was 4.5 (±2.9) months. Mean serum creatinine before and after SGLT-2i initiation was 1.46 (±0.42) and 1.58 (±0.55) mg/dL, respectively, with a median estimated glomerular filtration rate of 64 (±27) before and 64 (±32) mL/min/1.73 m2 after initiation of SGLT2i. Mean urinary albumin-creatinine ratio in mg/g creatinine before SGLT-2i initiation was 1827 (±1560) and decreased by almost 40% to 1127 (±854) after SGLT2i initiation. To our knowledge, this is the first case series on the effect and safety of SGLT2i in patients with hereditary podocytopathies. Specific large-scale trials in podocytopathies are needed to confirm our findings in this population with a tremendous unmet medical need for more effective, early on, and safe nephroprotective therapies.