bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical utility of plasma Aβ42/40 ratio by LC-MS/MS in Alzheimer’s disease assessment
Authors: Weber Darren M. , Taylor Steven W. , Lagier Robert J. , Kim Jueun C. , Goldman Scott M. , Clarke Nigel J. , Vaillancourt David E. , Duara Ranjan , McFarland Karen N. , Wang Wei-en , Golde Todd E. , Racke Michael K.
Published: March 2024

Introduction

Plasma Aβ42/40 ratio can help predict amyloid PET status, but its clinical utility in Alzheimer’s disease (AD) assessment is unclear.

Methods

Aβ42/40 ratio was measured by LC-MS/MS for 250 specimens with associated amyloid PET imaging, diagnosis, and demographic data, and for 6,192 consecutive clinical specimens submitted for Aβ42/40 testing.

Results

High diagnostic sensitivity and negative predictive value (NPV) for Aβ-PET positivity were observed, consistent with the clinical performance of other plasma LC-MS/MS assays, but with greater separation between Aβ42/40 values for individuals with positive vs. negative Aβ-PET results. Assuming a moderate prevalence of Aβ-PET positivity, a cutpoint was identified with 99% NPV, which could help predict that AD is likely not the cause of patients’ cognitive impairment and help reduce PET evaluation by about 40%.

Conclusion

High-throughput plasma Aβ42/40 LC-MS/MS assays can help identify patients with low likelihood of AD pathology, which can reduce PET evaluations, allowing for cost savings.
Optimal dose of fenfluramine in adjuvant treatment of drug-resistant epilepsy: evidence from randomized controlled trials
Authors: Xu Yingchun , Chen Deng , Liu Ling
Published: March 2024

Objective

Several clinical trials have suggested that fenfluramine (FFA) is effective for the treatment of epilepsy in Dravet syndrome (DS) and Lennox–Gastaut syndrome (LGS). However, the exploration of its optimal target dose is ongoing. This study aimed to summarize the best evidence to inform this clinical issue.

Materials and methods

We searched PubMed, Embase (via Ovid), and Web of Science for relevant literature published before December 1st, 2023. Randomized, double-blind, placebo-controlled studies that evaluated the efficacy, safety, and tolerability of FFA in DS and LGS were identified and meta-analysis was performed according to doses. The study was registered with PROSPERO (CRD42023392454).

Results

Six hundred and twelve patients from four randomized controlled trials were enrolled. The results demonstrated that FFA at 0.2, 0.4, or 0.7 mg/kg/d showed significantly greater efficacy compared to placebo in terms of at least 50% reduction (p < 0.001, p < 0.001, p < 0.001) and at least 75% reduction (p < 0.001, p = 0.007, p < 0.001) in monthly seizure frequency from baseline. Moreover, significantly more patients receiving FFA than placebo were rated as much improved or very much improved in CGI-I by both caregivers/parents and investigators (p < 0.001). The most common treatment-emergent adverse events were decreased appetite, diarrhea, fatigue, and weight loss, with no valvular heart disease or pulmonary hypertension observed in any participant. For dose comparison, 0.7 mg/kg/d group presented higher efficacy on at least 75% reduction in seizure (p = 0.006) but not on at least 50% reduction. Weight loss (p = 0.002), decreased appetite (p = 0.04), and all-cause withdrawal (p = 0.036) were more common in 0.7 mg/kg/d group than 0.2 mg/kg/d. There was no statistical difference in other safety parameters between these two groups.

Conclusion

The higher range of the licensed dose achieves the optimal balance between efficacy, safety, and tolerability in patients with DS and LGS.

Clinical trial registration

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42023392454.
Can molecular hydrogen supplementation reduce exercise-induced oxidative stress in healthy adults? A systematic review and meta-analysis
Authors: Li Yiting , Bing Renjie , Liu Meng , Shang Zhangyuting , Huang Yan , Zhou Kaixiang , Bao Dapeng , Zhou Junhong
Published: March 2024

Objective

Exercise-induced oxidative stress affects multiple neurophysiological processes, diminishing the exercise performance. Hydrogen (H2) can selectively reduce excessive free radicals, but studies observed its “dual effects” on exercise-induced oxidative stress, that is, increasing or decreasing the oxidative stress. Therefore, we here conducted a systematic review and meta-analysis to quantitatively assess the influence of H2 on exercise-induced oxidative stress in healthy adults.

Methods

We conducted a systematic review of publications across five databases. The following keywords were used for search strategy: [“hydrogen”[Mesh] or “molecular hydrogen” or “hydrogen rich water” or “hydrogen-rich water” or “hydrogen rich saline”] and [“Oxidative Stress”[Mesh] or “Antioxidative Stress” or “Oxidative Damage” or “Oxidative Injury” or “Oxidative Cleavage”] and [“randomized controlled trial”[Mesh] or “randomized” or “RCT”]. We included trials reporting the effects of H2 on exercise-induced oxidative stress and potential antioxidant capacity post-exercise in healthy adults. Additionally, subgroup analyses were conducted to explore how various elements of the intervention design affected those outcomes.

Results

Six studies, encompassing seven experiments with a total of 76 participants, were included in our analysis. Among these studies, hydrogen-rich water, hydrogen bathing, and hydrogen-rich gas were three forms used in H2 administration. The H2 was applied in different timing, including before, during, or after exercise only, both before and after exercise, and repeatedly over days. Single-dose, multi-dose within 1 day and/or multiple-dose over days were implemented. It was observed that compared to placebo, the effects of H2 on oxidative stress (diacron-reactive oxygen metabolites, d-ROMs) was not significant (SMD = −0.01, 95%CI-0.42 to 0.39, p = 0.94). However, H2 induced greater improvement in antioxidant potential capacity (Biological Antioxidant Potential, BAP) (SMD = 0.29, 95% CI 0.04 to 0.54, p = 0.03) as compared to placebo. Subgroup analyses revealed that H2 supplementation showed greater improvement (SMD = 0.52, 95%CI 0.16 to 0.87, p = 0.02) in the antioxidant potential capacity of intermittent exercises than continuous exercise.

Conclusion

H2 supplementation can help enhance antioxidant potential capacity in healthy adults, especially in intermittent exercise, but not directly diminish the levels of exercise-induced oxidative stress. Future studies with more rigorous design are needed to examine and confirm these findings.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=364123, Identifier CRD42022364123.
The anatomy, neurophysiology, and cellular mechanisms of intradental sensation
Authors: Ronan Elizabeth A. , Nagel Maximilian , Emrick Joshua J.
Published: March 2024

Somatosensory innervation of the oral cavity enables the detection of a range of environmental stimuli including minute and noxious mechanical forces. The trigeminal sensory neurons underlie sensation originating from the tooth. Prior work has provided important physiological and molecular characterization of dental pulp sensory innervation. Clinical dental experiences have informed our conception of the consequence of activating these neurons. However, the biological role of sensory innervation within the tooth is yet to be defined. Recent transcriptomic data, combined with mouse genetic tools, have the capacity to provide important cell-type resolution for the physiological and behavioral function of pulp-innervating sensory neurons. Importantly, these tools can be applied to determine the neuronal origin of acute dental pain that coincides with tooth damage as well as pain stemming from tissue inflammation (i.e., pulpitis) toward developing treatment strategies aimed at relieving these distinct forms of pain.
Field testing of recombinant subunit vaccines against Teladorsagia circumcincta in lambing ewes demonstrates a lack of efficacy in the face of a multi-species parasite challenge
Authors: Nisbet Alasdair J. , McNeilly Tom N. , Price Daniel R. G. , Bartley Yvonne , Oliver Margaret , McBean Dave , Andrews Leigh , Mitchell Gillian , Duncan Rachael , Brocklehurst Sarah , Kenyon Fiona
Published: March 2024

Introduction

We previously demonstrated efficacy of an 8-antigen recombinant subunit vaccine against a single species homologous Teladorsagia circumcincta challenge in lambs and in lambing ewes in pen trials. We subsequently demonstrated efficacy of a simplified, 2-antigen, version of this vaccine in lambs in pen trials. Here, we test both vaccines in lambing ewes in a field setting.

Methods

In the work presented here, 12 adjacent plots were seeded with a mixed infection of several common species of parasitic nematodes of sheep in temperate regions, including T. circumcincta. Ewes (n = 144), in groups of 12, grazed for 2 years on these plots and, in the first year, six of these groups of ewes were vaccinated with a 2-antigen prototype vaccine against T. circumcincta prior to mating and then again prior to lambing. In the following year these ewes were immunised again, this time with the 8-antigen prototype vaccine against T. circumcincta prior to mating and then prior to lambing. Throughout both seasons antigen-specific serum antibody levels in ewes and faecal worm egg counts (FEC) in ewes and their lambs were monitored, along with nematode species diversity at lambing.

Results

Immunised ewes produced elevated serum antibody levels to each of the vaccine antigens following immunisation but their FEC levels were not statistically significantly impacted by vaccination with either vaccine. FEC levels were also not impacted in lambs co-grazing the pastures with these immunised ewes. Nematode species diversity was not significantly impacted by vaccination in either year.

Discussion

The immunosuppressive effects of co-infecting gastrointestinal nematodes, the absence of vaccine cross-protection against co-infecting species and the influence of the periparturient relaxation in immunity probably all contributed to the inability of either vaccine to protect against T. circumcincta infection in field trials in the work presented here.
Ocular pharmacological and biochemical profiles of 6-thioguanine: a drug repurposing study
Authors: Trotta Maria Consiglia , Gesualdo Carlo , Lepre Caterina Claudia , Russo Marina , Ferraraccio Franca , Panarese Iacopo , Marano Ernesto , Grieco Paolo , Petrillo Francesco , Hermenean Anca , Simonelli Francesca , D’Amico Michele , Bucolo Claudio , Lazzara Francesca , De Nigris Filomena , Rossi Settimio , Platania Chiara Bianca Maria
Published: March 2024

Introduction:

The purine analog 6-thioguanine (6TG), an old drug approved in the 60s to treat acute myeloid leukemia (AML), was tested in the diabetic retinopathy (DR) experimental in vivo setting along with a molecular modeling approach.

Methods:

A computational analysis was performed to investigate the interaction of 6TG with MC1R and MC5R. This was confirmed in human umbilical vein endothelial cells (HUVECs) exposed to high glucose (25 mM) for 24 h. Cell viability in HUVECs exposed to high glucose and treated with 6TG (0.05–0.5–5 µM) was performed. To assess tube formation, HUVECs were treated for 24 h with 6TG 5 µM and AGRP (0.5–1–5 µM) or PG20N (0.5–1–5–10 µM), which are MC1R and MC5R antagonists, respectively. For the in vivo DR setting, diabetes was induced in C57BL/6J mice through a single streptozotocin (STZ) injection. After 2, 6, and 10 weeks, diabetic and control mice received 6TG intravitreally (0.5–1–2.5 mg/kg) alone or in combination with AGRP or PG20N. Fluorescein angiography (FA) was performed after 4 and 14 weeks after the onset of diabetes. After 14 weeks, mice were euthanized, and immunohistochemical analysis was performed to assess retinal levels of CD34, a marker of endothelial progenitor cell formation during neo-angiogenesis.

Results:

The computational analysis evidenced a more stable binding of 6TG binding at MC5R than MC1R. This was confirmed by the tube formation assay in HUVECs exposed to high glucose. Indeed, the anti-angiogenic activity of 6TG was eradicated by a higher dose of the MC5R antagonist PG20N (10 µM) compared to the MC1R antagonist AGRP (5 µM). The retinal anti-angiogenic effect of 6TG was evident also in diabetic mice, showing a reduction in retinal vascular alterations by FA analysis. This effect was not observed in diabetic mice receiving 6TG in combination with AGRP or PG20N. Accordingly, retinal CD34 staining was reduced in diabetic mice treated with 6TG. Conversely, it was not decreased in diabetic mice receiving 6TG combined with AGRP or PG20N.

Conclusion:

6TG evidenced a marked anti-angiogenic activity in HUVECs exposed to high glucose and in mice with DR. This seems to be mediated by MC1R and MC5R retinal receptors.
The patterns of inbreeding depression in food-deceptive Dactylorhiza orchids
Authors: Wróblewska Ada , Ostrowiecka Beata , Brzosko Emilia , Jermakowicz Edyta , Tałałaj Izabela , Mirski Paweł
Published: March 2024

Introduction

Inbreeding depression (ID) in food-deceptive plants have been reported previously, however, it has not been often proven that selfed seeds germinate better than outbred ones or that selfing affects ID. To resolved these issues, food-deceptive related Dactylorhiza majalis, D. incarnata var. incarnata and D. fuchsii orchids were investigated.

Methods

Hand pollination treatments and control pollination were conducted. Fruit set, number of seeds per fruit, seed length, number of well-developed seeds per fruit, and proportion of in vitro asymbiotic germination seeds, were analyzed in relation to inflorescence levels and used as fitness indicators for these orchids. The ID and pollen limitation were measured.

Results

The lowest ID (δ = −1.000) was in D. majalis, and present in combination with a high pollen limitation in its populations. D. fuchsii showed higher ID (δ = 0.366), and D. incarnata var. incarnata weak ID (δ = 0.065), although ID varied between its fitness components. The seed number per fruit differed significantly between the treatments and the inflorescence levels in treatments.

Discussion

This study emphasizes that the breeding system rather than the flower position on the inflorescence shaped the quality and quantity of reproductive output. The ID and its effect on germination of food-deceptive orchid seeds undoubtedly played an important role.
Shallow wet irrigation reduces nitrogen leaching loss rate in paddy fields by microbial regulation and lowers rate of downward migration of leaching water: a 15N-tracer study
Authors: Chen Tianyi , Yang Xiaoming , Zuo Zheng , Xu Huijuan , Yang Xingjian , Zheng Xiangjian , He Shuran , Wu Xin , Lin Xueming , Li Yongtao , Zhang Zhen
Published: March 2024

China consumes 35% of the world’s fertilizer every year; however, most of the nitrogen fertilizers, which are essential for rice cultivation, are not used effectively. In this study, factors affecting the nitrogen leaching loss rate were studied in typical soil and rice varieties in South China. The effects of various irrigation measures on rice growth and nitrogen leaching loss were investigated by conducting experiments with eight groups. These groups included traditional irrigation (TI) and shallow wet irrigation (SWI). The TI is a common irrigation method for farmers in South China, maintaining a water layer of 5-8 cm depth. For SWI, after establishing a shallow water layer usually maintaining at 1-2 cm, paddy is irrigated when the field water level falls to a certain depth, then this process is then repeat as necessary. The nitrogen distribution characteristics were determined using 15N isotope tracing. In addition, the effects of nitrification, denitrification, and microbial composition on soil nitrogen transformation at different depths were studied by microbial functional gene quantification and high-throughput sequencing. The results revealed that in the SWI groups, the total nitrogen leaching loss rate reduced by 0.3-0.8% and the nitrogen use efficiency (NUE) increased by 2.18-4.43% compared with those in the TI groups. After the 15N-labeled nitrogen fertilizer was applied, the main pathways of nitrogen were found to be related to plant absorption and nitrogen residues. Furthermore, paddy soil ammonia-oxidizing archaea were more effective than ammonia-oxidizing bacteria for soil ammonia oxidation by SWI groups. The SWI measures increased the relative abundance of Firmicutes in paddy soil, enhancing the ability of rice to fix nitrogen to produce ammonium nitrogen, thus reducing the dependence of rice on chemical fertilizers. Moreover, SWI enhanced the relative abundance of nirS and nosZ genes within surface soil bacteria, thereby promoting denitrification in the surface soil of paddy fields. SWI also promoted ammonia oxidation and denitrification by increasing the abundance and activity of Proteobacteria, Nitrospirae, and Bacteroidetes. Collectively, SWI effectively reduced the nitrogen leaching loss rate and increase NUE.
GRIK phosphorylates and activates KIN10 which also promotes its degradation
Authors: Sun Jing , Liu Hui , Blanford Jantana K. , Cai Yingqi , Zhai Zhiyang , Shanklin John
Published: March 2024

The sensor kinase Sucrose Non-fermenting-1-Related Kinase 1 (SnRK1) plays a central role in energy and metabolic homeostasis. KIN10 is a major catalytic (α) kinase subunit of SnRK1 regulated by transcription, posttranslational modification, targeted protein degradation, and its subcellular localization. Geminivirus Rep Interacting Kinase 1 and 2 (GRIK1 and 2) are immediate upstream kinases of KIN10. In the transient protein expression assays carried out in Nicotiana benthamiana (N. benthamiana) leaves, GRIK1 not only phosphorylates KIN10 but also simultaneously initiates its degradation. Posttranslational GRIK-mediated KIN10 degradation is dependent on both GRIK kinase activity and phosphorylation of the KIN10 T-loop. KIN10 proteins are significantly enriched in the grik1-1 grik2-1 double mutant, consistent with the transient assays in N. benthamiana. Interestingly. Among the enriched KIN10 proteins from grik1-1 grik2-1, is a longer isoform, putatively derived by alternative splicing which is barely detectable in wild-type plants. The reduced stability of KIN10 upon phosphorylation and activation by GRIK represents a mechanism that enables the KIN10 activity to be rapidly reduced when the levels of intracellular sugar/energy are restored to their set point, representing an important homeostatic control that prevents a metabolic overreaction to low-sugar conditions. Since GRIKs are activating kinases of KIN10, KIN10s in the grik1 grik2 double null mutant background remain un-phosphorylated, with only their basal level of activity, are more stable, and therefore increase in abundance, which also explains the longer isoform KIN10L which is a minor isoform in wild type is clearly detected in the grik1 grik2 double mutant.
Unraveling the genetics of heat tolerance in chickpea landraces (Cicer arietinum L.) using genome-wide association studies
Authors: Danakumara Thippeswamy , Kumar Neeraj , Patil Basavanagouda Siddanagouda , Kumar Tapan , Bharadwaj Chellapilla , Jain Pradeep Kumar , Nimmy Manduparambil Subramanian , Joshi Nilesh , Parida Swarup Kumar , Bindra Shayla , Kole Chittaranjan , Varshney Rajeev K.
Published: March 2024

Chickpea, being an important grain legume crop, is often confronted with the adverse effects of high temperatures at the reproductive stage of crop growth, drastically affecting yield and overall productivity. The current study deals with an extensive evaluation of chickpea genotypes, focusing on the traits associated with yield and their response to heat stress. Notably, we observed significant variations for these traits under both normal and high-temperature conditions, forming a robust basis for genetic research and breeding initiatives. Furthermore, the study revealed that yield-related traits exhibited high heritability, suggesting their potential suitability for marker-assisted selection. We carried out single-nucleotide polymorphism (SNP) genotyping using the genotyping-by-sequencing (GBS) method for a genome-wide association study (GWAS). Overall, 27 marker–trait associations (MTAs) linked to yield-related traits, among which we identified five common MTAs displaying pleiotropic effects after applying a stringent Bonferroni-corrected p-value threshold of <0.05 [−log10(p) > 4.95] using the BLINK (Bayesian-information and linkage-disequilibrium iteratively nested keyway) model. Through an in-depth in silico analysis of these markers against the CDC Frontier v1 reference genome, we discovered that the majority of the SNPs were located at or in proximity to gene-coding regions. We further explored candidate genes situated near these MTAs, shedding light on the molecular mechanisms governing heat stress tolerance and yield enhancement in chickpeas such as indole-3-acetic acid–amido synthetase GH3.1 with GH3 auxin-responsive promoter and pentatricopeptide repeat-containing protein, etc. The harvest index (HI) trait was associated with marker Ca3:37444451 encoding aspartic proteinase ortholog sequence of Oryza sativa subsp. japonica and Medicago truncatula, which is known for contributing to heat stress tolerance. These identified MTAs and associated candidate genes may serve as valuable assets for breeding programs dedicated to tailoring chickpea varieties resilient to heat stress and climate change.
Exploring the prevalence and predictors of low resilience and likely PTSD in residents of two provinces in Canada during the 2023 wildfires
Authors: Adu Medard K. , Shalaby Reham , Agyapong Belinda , Dias Raquel da Luz , Agyapong Vincent I. O.
Published: March 2024

Background

The recent wildfires in Canada serve as a stark example of the substantial and enduring harm they cause to the health of individuals and communities. Assessing the prevalence and correlates of Post-traumatic stress disorder (PTSD) and low resilience is valuable for policymakers in public health.

Objectives

The study aimed to assess the prevalence and predictors of low resilience and likely PTSD among subscribers of Text4Hope, an e-mental health program that delivered daily supportive messages to residents of Nova Scotia (NS) and Alberta(AB) during the recent wildfires.

Method

Data collection was through a self-administered online survey completed by residents of the affected regions of NS and AB from May 14 to June 23, 2023. Data were analyzed using Statistical Package for the Social Sciences.

Results

Out of 298 respondents, the prevalence of low resilience and likely PTSD in our sample were 52.0 and 39.3%, respectively. Unemployed respondents were about 3 times more likely to experience both low resilience and PTSD symptoms compared to those employed. Respondents with a history of mental health diagnosis were about 4 times more likely to experience likely PTSD compared to those with no history of mental health diagnosis.

Conclusion

This study established that unemployment and a history of mental health diagnosis predicted likely PTSD, while unemployment was associated with low resilience during the wildfire. These findings offer insights for clinical interventions and the creation of psychosocial support programs for vulnerable populations.
The Mediating Role of Attitudes towards Performing Well between Ethical Leadership, Technological Innovation, and Innovative Performance
Authors: Aslam Hassan Danial , Căpușneanu Sorinel , Javed Tasawar , Rakos Ileana-Sorina , Barbu Cristian-Marian
Published: March 2024

The business sector is rife with unethical managerial practices, such as blaming subordinates for organizational failings, along with the exploitation of colleagues, favoritism, and conflicts of leadership. In light of this, numerous researchers have endeavored to explain the phenomenon of ethical perspectives and concerns. It is recommended that organizations in the SME sector adopt ethical practices in order to achieve their long-term objectives. This study stresses the influence of ethical leadership and technological innovation, particularly the mediating role of a positive attitude in encouraging good performance. This study, which was conducted in the SME sector of Malaysia in the state of Selangor, employed a convenience sampling technique for data collection and reports that ethical leadership, technological innovation, and attitude significantly influence performance. A mediating role of attitude in relation to technological innovation and innovative performance is hereby reported, and attitude towards performance was found to have an insignificant effect on ethical leadership and innovative performance. This study contributes to the existing body of knowledge by explaining the role of ethical leadership and technological innovation in expressing and assessing the revolutionary business practices required to ensure organizational success.
Comparative Study of Numerical Schemes for Granular Combustion of Boron Potassium Nitrate
Authors: Elizabeth Annie Rose , Sarma Sumit , Jayachandran T. , Ramakrishna P. A. , Borthakur Mondeep
Published: March 2024

Multiple applications in aerospace utilize pyrotechnic charges for their operation, and these charges are predominantly in the form of granules. One of the most used charges is boron potassium nitrate (BPN), and the present study focuses on mathematically modeling granular combustion, its experimental recreation, and carrying out a comparative study on three upwind schemes for its numerical simulation. A customized version of the seven-equation compressible multifluid formulation is presented in this paper to model granular combustion mathematically. Three upwind schemes, namely HLLC, AUSM+-up, and HLLC-AUSM, are used for the numerical comparison. Utilizing these, an axisymmetric code is developed for the comparative study. To experimentally replicate granular combustion, granular BPN is fired in a closed bomb test facility, and the experimental pressure history is used for the numerical comparisons. The developed code can adequately predict the physics of granular combustion, and all three schemes are equally capable of numerical prediction.
A Nonlinear Beam Finite Element with Bending–Torsion Coupling Formulation for Dynamic Analysis with Geometric Nonlinearities
Authors: Patuelli Cesare , Cestino Enrico , Frulla Giacomo
Published: March 2024

Vibration analysis of wing-box structures is a crucial aspect of the aeronautic design to avoid aeroelastic effects during normal flight operations. The deformation of a wing structure can induce nonlinear couplings, causing a different dynamic behavior from the linear counterpart, and nonlinear effects should be considered for more realistic simulations. Moreover, composite materials and aeroelastic tailoring require new simulation tools to include bending–torsion coupling effects. In this research, a beam finite element with bending–torsion coupling formulation is used to investigate the effects of the deflection of beam structures with different aspect ratios. The nonlinear effects are included in the finite element formulation. The geometrical effect is considered, applying a deformation dependent transformation matrix. Stiffness effects are introduced in the stiffness matrix with Hamilton’s Principle and a perturbation approach. The results obtained with the beam finite element model are compared with numerical and experimental evidence.
Parametric Design Method and Lift/Drag Characteristics Analysis for a Wide-Range, Wing-Morphing Glide Vehicle
Authors: Jin Zikang , Yu Zonghan , Meng Fanshuo , Zhang Wei , Cui Jingzhi , He Xiaolong , Lei Yuedi , Musa Omer
Published: March 2024

The parametric design method is widely utilized in the preliminary design stage for hypersonic vehicles; it ensures the fast iteration of configuration, generation, and optimization. This study proposes a novel parametric method for a wide-range, wing-morphing glide vehicle. The whole configuration, including a waverider fuselage, a rotating wing, a blunt leading edge, rudders, etc., can be easily described using 27 key parameters. In contrast to the typical parametric method, the new method takes internal payloads into consideration during the shape optimization process. That is, the vehicle configuration can be flexibly adjusted depending on the internal payloads; these payloads may be of random amounts and have different shapes. The code for the new parametric design method is developed using the secondary development tools of UG (UG 10.0) commercial software. The lift and drag characteristics over a wide operational range (H = 6–25 km, M = 2.5–8.5, AOA = 0–10°) were numerically investigated, as was the influence of the retracting angle of the morphing wings. It was found that, for the mode of the fully deployed wings, the lift-to-drag ratio (L/D) remained at a high level (≥4.7) over a Mach range of 4.0–8.5 and an AOA range of 4–7°. For the mode of the fully retracted wings, the drag coefficient remained smaller than 0.02 over a Mach range of 4.0–8.5 and an AOA range of 0–5°. A wide L/D of 0.3–4.7 could be achieved by controlling the retracting angle of the wings, thus demonstrating a good potential for flight maneuverability. The flexible change in L/D proved to be a combined result of varying pressure distribution and edge-flow spillage. This will aid in the further optimization of lift/drag characteristics.
Enhancing Wheat Sprout Attributes Using “Intensification of Vaporization by Decompression to the Vacuum”, an Innovative Drying–Texturizing Technology
Authors: Francis Helga , Debs Espérance , Maroun Richard G. , Louka Nicolas
Published: March 2024

Highly valued for their nutritional benefits, sprouts are characterized by high water content, which promotes microbial proliferation, potentially leading to toxicity and a reduced shelf life. To address this challenge, the present study explores the application of a novel drying–texturizing approach, named IVDV (Intensification of Vaporization by Decompression to the Vacuum), to sprouts. This technique would enable faster drying of the sprouts and better preservation of their nutritional content, compared to traditional hot-air drying. Using Response Surface Methodology, optimal IVDV parameters (saturated steam pressure P, processing time t, and water content W) for wheat sprouts were defined, with a focus on preserving vitamins, proteins, and lipids, and optimizing the expansion ratio. This optimization process identified optimal experimental conditions at 5.5 bars, a duration of 15 s, and 8.8% d.b. water content. Under these conditions, the use of IVDV endowed the expanded sprouts with a crunchier and more friable texture. In addition, it significantly improved the preservation of vitamins B6 and E by 412% and 42%, respectively, compared to traditional mild hot-air drying, without significantly affecting vitamin B2, proteins, and lipids. When combined with conventional hot-air drying, IVDV not only enhanced the preservation of the sprouts’ nutritional content but also reduced drying time and energy consumption. This marks a significant advancement in sprouts preservation techniques, paving the way for novel potential applications.
Next-Generation Biofertilizers: Nanoparticle-Coated Plant Growth-Promoting Bacteria Biofertilizers for Enhancing Nutrient Uptake and Wheat Growth
Authors: Karunakaran Anagha , Fathima Yaraa , Singh Pallavi , Beniwal Rahul , Singh Jyoti , Ramakrishna Wusirika
Published: March 2024

Contemporary agricultural practices rely heavily on synthetic fertilizers to provide essential nutrients for crops, contributing to diminished soil fertility and environmental pollution. An innovative solution lies in the strategic combination of nanoparticles and biofertilizers, as a unique and environmentally friendly technology, enhancing soil enzyme activity and the availability of essential plant nutrients. The goal of this study was to show the efficacy of this technology and identify the best combination of nanoparticles and PGPB for plant growth promotion, nutrient uptake, and soil health. This study investigated the efficacy of nanobiofertilizers generated by combining two plant growth-promoting bacteria (PGPB), (Bacillus sp.) CP4 and AHP3, along with mesoporous silica nanoparticles (MS NPs), zinc oxide nanoparticles (ZnO NPs), and copper oxide nanoparticles (CuO NPs) in different combinations. A greenhouse study employing two wheat varieties, NABI MG11 (black wheat) and HD3086, was conducted. There were 15 treatments, including treatments consisting of only bacteria, treatments consisting of the combination of nanoparticles and nanobiofertilizers, and 1 control treatment, and each treatment had three replicates. In evaluating plant growth characteristics, the synergy between ZnO NPs and CP4 demonstrated the most favorable outcomes in terms of overall plant growth and various traits. Similarly, MS NPs, in conjunction with both PGPB, exhibited enhancements in plant growth traits, including fresh weight, chlorophyll content, proline levels, and nitrogen content. Over half of the combination treatments with nanoparticles and PGPB did not show a significant improvement in plant growth promotion traits and soil health when compared to nanoparticles alone. The findings of this study underscore the potential of nanobiofertilizers as an innovative and robust tool for promoting sustainable agriculture.
Transcript Analysis Reveals Positive Regulation of CA12g04950 on Carotenoids of Pigment Pepper Fruit under Nitrogen Reduction
Authors: Shen Lingfeng , Zhang Chenfei , Xia Yahui , Yang Shasha , Chang Tian , Ullah Saleem , Ji Xuehua
Published: March 2024

This study investigates the relationship between nitrogen fertilization and pepper fruit color by employing five different nitrogen treatments (N1: 750 kg/hm2, N2: 562.5 kg/hm2, N3: 375 kg/hm2, N4: 187.5, and N0: 0 kg/hm2). Fruits were harvested at 30 (S1: green ripening stage), 45 (S2: color transition stage), and 60 days (S3: red ripening stage) after flowering. Subsequently, pigment content, carotenoid component content, carotenoid enzyme activity, and transcriptome sequence were analyzed, and CA12g04950 function was validated through virus-induced gene silencing (VIGS). The results indicate that a reduction in nitrogen application led to an earlier onset of fruit color breakdown, and increased the contents of total carotenoid, capsanthin, phytoene and PSY (phytoene synthase) activity, LCYB (lycopene β-cyclase) activity and CCS (capsanthin/capsorubin synthase) activity. The analysis of different expression genes indicated that the most differently expressed genes were enriched in the N1 vs. N4 comparison, with 18 genes involved in carotenoid metabolism and 16 genes involved in nitrogen metabolism. Most DE genes were enriched in the pathways of photosynthesis, porphyrin, carotenoid biosynthesis, seleno-compounds, and nitrogen metabolism. There were numerous differential transcription factor families, including ERF, bHLH, MYB, C2H2, and NAC. Pearson correlation analysis revealed a significant positive correlation between CA12g04950 expression and 11 carotenoid genes in the N4 treatment. Subsequent silencing of CA12g04950 using VIGS resulted in delayed color ripening while a significant decrease in total carotenoid content and the expression levels of carotenoid genes. In conclusion, nitrogen reduction led to an increase in carotenoid content in pigment pepper fruits. Furthermore, under nitrogen reduction, CA12g04950 positively influenced the redness of the fruits.
Non-Destructive Classification of Maize Seeds Based on RGB and Hyperspectral Data with Improved Grey Wolf Optimization Algorithms
Authors: Bi Chunguang , Zhang Shuo , Chen He , Bi Xinhua , Liu Jinjing , Xie Hao , Yu Helong , Song Shaozhong , Shi Lei
Published: March 2024

Ensuring the security of germplasm resources is of great significance for the sustainable development of agriculture and ecological balance. By combining the morphological characteristics of maize seeds with hyperspectral data, maize variety classification has been achieved using machine learning algorithms. Initially, the morphological data of seeds are obtained from images, followed by the selection of feature subsets using Recursive Feature Elimination (RFE) and Select From Model (SFM) methods, indicating that features selected by RFE exhibit better performance in maize seed classification. For hyperspectral data (350–2500 nm), Competitive Adaptive Re-weighted Sampling (CARS) and the Successive Projections Algorithm (SPA) are employed to extract feature wavelengths, with the SPA algorithm demonstrating superior performance in maize seed classification tasks. Subsequently, the two sets of data are merged, and a Random Forest (RF) classifier optimized by Grey Wolf Optimization (GWO) is utilized. Given the limitations of GWO, strategies such as logistic chaotic mapping for population initialization, random perturbation, and final replacement mechanisms are incorporated to enhance the algorithm’s search capabilities. The experimental results show that the proposed ZGWO-RF model achieves an accuracy of 95.9%, precision of 96.2%, and recall of 96.1% on the test set, outperforming the unimproved model. The constructed model exhibits improved identification effects on multi-source data, providing a new tool for non-destructive testing and the accurate classification of seeds in the future.
Quantifying High-Temperature-Induced Injury in Nanfeng Tangerine Plants: Insights from Photosynthetic and Biochemical Mechanisms
Authors: Xu Chao , Wang Yuting , Yang Huidong , Tang Yuqing , Liu Xincheng , Liu Buchun , Hu Xinlong , Hu Zhongdong
Published: March 2024

High temperatures significantly injure the flowering, pollination, fruit growth, and quality of plants. Photosynthesis, the fundamental process supporting plant life, is crucial. Nevertheless, the quantitative evaluation of the physiological activity of the photosynthetic system of Nanfeng tangerine (NT) plants under high-temperature conditions remains a challenge. This research utilized NT plants, a distinctive citrus variety in Jiangxi Province, as the experimental subject. The study investigated the effects of varying degrees of high-temperature stress and duration on 16 photosynthetic physiological parameters of NT plants. The study examined the impact of four varying high-temperature treatment levels (32/22 °C, 35/25 °C, 38/28 °C, and 41/31 °C) for durations of 2, 4, 6, and 8 days, respectively. Principal component analysis was utilized to identify the key indicators of photosynthetic physiological activity in NT plants, with Fv/Fm, Pmax, LCP, H2O2, MDA, and POD being selected as key parameters. The high-temperature stress index model previously constructed was used to calculate the high-temperature stress index value of the NT plants exposed to varying degrees and durations of high temperature, in order to provide a comprehensive assessment of the photosynthetic system of NT plants under high-temperature stress. Subsequently, the high-temperature stress levels were categorized into five levels based on the calculated values: Level 0 for 0 < HSI ≤ 2, Level 1 for 2 < HSI ≤ 4, Level 2 for 4 < HSI ≤ 6, Level 3 for 6 < HSI ≤ 8, and Level 4 for HSI > 8. The research results provide valuable data for agricultural meteorological departments to carry out disaster risk zoning and risk assessment in the future.
Combined Application of Chemical and Organic Fertilizers: Effects on Yield and Soil Nutrients in Spring Wheat under Drip Irrigation
Authors: Chang Xiangjie , He Hao , Cheng Liyang , Yang Xiaojuan , Li Shuai , Yu Mengmeng , Zhang Jifeng , Li Junhua
Published: March 2024

In this study, we established a feasible fertilization programming method for wheat production by exploring the effects of the combined application of chemical and organic fertilizers on wheat yield, nutrient uptake, soil nutrient content, and fertilizer utilization. Six treatments, no fertilizer (CK), conventional fertilizer (CF), optimized fertilizer (with reduced fertilizer amount) (RF), chemical fertilizer with organic fertilizer extract (RPAE), partial replacement of chemical fertilizer with raw amino acid powder (RAF), and partial replacement of chemical fertilizer with raw humic acid powder (RHF), were set up for a field experiment. The fertilizer application rates for the RF treatment were calculated based on fertilization-monitoring techniques (30.3% nitrogen and 24.8% phosphorus reductions in 2022 and 23.0% nitrogen and 1.5% phosphorus reductions in 2023). The effects of different fertilizer treatments on yield, dry matter accumulation, plant nutrient accumulation, soil nutrients, and nutrient utilization in wheat were investigated. The results showed that, on the basis of 23% nitrogen and 1.5% phosphorus reductions, there was no significant difference in wheat yield between the RF and CF treatments and that the utilization rate of nitrogen fertilizer was improved. The application of organic fertilizer promoted dry matter accumulation in different organs of wheat; increased plant nutrient accumulation; improved soil nutrient content, nutrient utilization rate, nutrient partial productivity, and nutrient agronomic use efficiency; and ensured stable and increased crop yield. Specifically, compared with CF, the RPAE, RAF, and RHF organic fertilizer treatments increased wheat yield by 3.85%, 1.97%, and 0.67%, respectively, and the utilization of nitrogen and phosphorus fertilizers induced by these treatments significantly increased by 40.46%, 39.28%, and 37.46% (nitrogen) and by 9.83%, 8.91%, and 7.46% (phosphorus), respectively. As a result of our experiment, we concluded that RPAE exerted the best effects among the three organic fertilizer treatments (RPAE, RAF, and RHF) and that its use can result in a higher wheat yield and fertilizer utilization rate in drip-irrigated wheat fields. The results of this study provide a theoretical basis for the combined application of chemical and organic fertilizers, which is conducive to sustainable agriculture development.
Phytophthora Infection Reorients the Composition of Rhizospheric Microbial Assembly in Khasi Mandarin (Citrus reticulata Blanco)
Authors: Handique Mridupol , Bora Popy , Ziogas Vasileios , Srivastava Anoop Kumar , Jagannadham Prasanth Tej Kumar , Das Asish Kumar
Published: March 2024

Phytophthora gummosis, foot rot, and root rot are considered major challenges to the citrus industry worldwide. Little is known about the Phytophthora–microbiome interaction, despite several studies demonstrating changes in the microbial composition of the rhizosphere following challenges by a pathogen. In the present study, we studied the microbial diversity and community structure in healthy rhizospheres and Phytophthora-infected rhizospheres of Khasi mandarin (Citrus reticulata Blanco), a commercial cultivar extensively grown in the northeast of India. An exploratory study was conducted to identify Phytophthora-infected orchards of Khasi mandarin, and the isolated pathogen was confirmed as P. nicotianae based on its morpho-cultural and molecular characteristics coupled with pathogenicity tests. This study on culturable microbes established the dominance of Trichoderma spp. in the healthy rhizosphere, while the diseased rhizosphere showed the presence of Fusarium spp. A metagenomic study further revealed that the rhizospheres of Phytophthora-infected plants were dominated by species such as Bacteroidia spp., Patescibacteria spp., and Pythium spp., while the healthy Khasi mandarin rhizospheres had a more diverse community predominantly represented by Trichoderma, Penicillium, Linnemannia, Mortierella, Talaromyces, Saitozyma, Bacteroidetes, Pseudomonas, Cytophagia, Cyanobacteria, Bacteroidia, Sphingobacteriia, Burkholderia, Bacillus, and Bradyrhizobium. Terrabacteria and FCB (Fibrobacterota, Chlorobiota, and Bacteroidota groups) were found to exist in higher relative abundance in disease-free soils than in Phytophthora-infected soils, while phylum Proteobacteria showed identical relative abundance in all soil types. The phyla represented by Pseudomonas, Flavobacteriia, Candidatus, Mycobacterium, Rhizobium, Mesorhizobium, Sphingomonas, and Cytophagia were the most common bacterial phyla in all soil samples, but healthy soil exhibited a greater abundance of Bacteroidetes, Pseudomonas, Cytophagia, Cyanobacteria, Bacteroidia, Sphingobacteriia, Burkholderia, Bacillus, and Bradyrhizobium. Our study suggests that the presence of Phytophthora spp. in the rhizosphere alters microbial community structure, having potentially strong implications for plant health and productivity. These rhizosphere microbiome-derived citrus responses shed light on exploring effective management strategies for Phytophthora gummosis disease ailing Khasi mandarin.
Elucidating the Response of Diverse Faba Bean Genotypes to Powdery Mildew Infection
Authors: Villegas-Fernández Ángel M. , García Lucía , Barilli Eleonora , Rispail Nicolas , Rubiales Diego
Published: March 2024

Faba bean (Vicia faba) is a temperate grain legume of major importance for food and feed. Powdery mildews are an important group of diseases in many crops, although in faba bean, it is still considered to be of only minor and local relevance. Here, we report the occurrence of powdery mildew in southern Spain, which was identified through ITS sequencing as Erysiphe trifolii. Resistance screenings allowed the identification of a wide range of responses to the disease, with accessions BPL-710 and ILB-4708 outstanding due to their high levels of resistance. Histological studies showed that the mechanisms of resistance may involve the inhibition of germination and impairment of fungal development, as shown by a limited number of primary and secondary hyphae compared to those of the susceptible accessions. This work permitted a better understanding of the interaction of faba bean and powdery mildew, laying the ground for breeding programs for resistance if needed in the future.
Current Status of Trypanosoma grosi and Babesia microti in Small Mammals in the Republic of Korea
Authors: Kim Hyun Jung , Han BoGyeong , Lee Hee-Il , Ju Jung-Won , Shin Hyun-Il
Published: March 2024

Small mammals, such as rodents and shrews, are natural reservoir hosts of zoonotic diseases, including parasitic protozoa. To assess the risk of rodent-borne parasitic protozoa in the Republic of Korea (ROK), this study investigated the status of parasitic protozoa, namely Trypanosoma, Babesia, and Theileria, in small mammals. In total, 331 blood samples from small mammals were analyzed for parasites using PCR and sequenced. Samples were positive for Trypanosoma grosi (23.9%; n = 79) and Babesia microti (10%; n = 33) but not Theileria. Small mammals from Seogwipo-si showed the highest infection rate of T. grosi (48.4%), while the highest B. microti infection rate was observed in those from Gangneung-si (25.6%). Sequence data revealed T. grosi to be of the AKHA strain. Phylogenetic analysis of B. microti revealed the US and Kobe genotypes. B. microti US-type–infected small mammals were detected throughout the country, but the Kobe type was only detected in Seogwipo-si. To our knowledge, this is the first nationwide survey that confirmed T. grosi and B. microti infections at the species level in small mammals in the ROK and identified the Kobe type of B. microti. These results provide valuable information for further molecular epidemiological studies on these parasites.