bluuebunny/crossref_metadata_2025 huggingface dataset:
First-in-human study to investigate the safety and pharmacokinetics of salvianolic acid A and pharmacokinetic simulation using a physiologically based pharmacokinetic model
Authors: Chen Jinliang , Ruan Zourong , Lou Honggang , Yang Dandan , Shao Rong , Xu Yichao , Hu Xinhua , Jiang Bo
Published: November 2022

Salvianolic acid A (SAA) is a water-soluble phenolic acid component from Salvia miltiorrhiza Bunge currently under development for myocardial protection treatment for coronary heart disease (CHD). We investigated the safety, tolerability, and pharmacokinetics of single and multiple ascending doses of SAA. Additionally, a physiologically based pharmacokinetic (PBPK) model was developed to simulate the pharmacokinetics of SAA. This was a first-in-human (FIH), randomized, double-blind, placebo-controlled, single, and multiple-dose study in 116 healthy Chinese subjects with the range of 10–300 mg and 60–200 mg SAA, respectively. SAA was well tolerated at all dose levels, following both single and multiple doses, with a low overall incidence of treatment-emergent adverse events (TEAEs) which appeared to be no dose-related. The main pharmacokinetic parameter of SAA, assessed by the power model, was the lack of proportionality with the dose range after single dosing. The 90% CIs of the slope β of Cmax (1.214 [1.150–1.278]) and AUC0-t (1.222 [1.156–1.288]) were not within the predefined acceptance range, and the direction of the deviation was higher than expected. PBPK modeling suggested the transfer ability saturation of hepatic organic anion-transporting polypeptide 1B1 (OATP1B1) and P-glycoprotein (P-gp) might result in a relatively low distribution rate at higher doses. Clinical plasma concentrations observed were in good agreement with PBPK prediction. SAA showed well-characterized pharmacokinetics and was generally well tolerated in the dose range investigated. The PBPK model provides valuable pharmacokinetic knowledge for further clinical development.
YOLOX-based blue laser weeding robot in corn field
Authors: Zhu Huibin , Zhang Yuanyuan , Mu Danlei , Bai Lizhen , Zhuang Hao , Li Hui
Published: November 2022

A YOLOX convolutional neural network-based weeding robot was designed for weed removal in corn seedling fields, while verifying the feasibility of a blue light laser as a non-contact weeding tool. The robot includes a tracked mobile platform module, a weed identification module, and a robotic arm laser emitter module. Five-degree-of-freedom robotic arm designed according to the actual weeding operation requirements to achieve precise alignment of the laser. When the robot is in operation, it uses the texture and shape of the plants to differentiate between weeds and corn seedlings. The robot then uses monocular ranging to calculate the coordinates of the weeds using the triangle similarity principle, and it controls the end actuator of the robotic arm to emit the laser to kill the weeds. At a driving speed of 0.2 m·s-1 on flat ground, the weed robot’s average detection rate for corn seedlings and weeds was 92.45% and 88.94%, respectively. The average weed dry weight prevention efficacy was 85%, and the average seedling injury rate was 4.68%. The results show that the robot can accurately detect weeds in corn fields, and the robotic arm can precisely align the weed position and the blue light laser is effective in removing weeds.
A comparison of classical and machine learning-based phenotype prediction methods on simulated data and three plant species
Authors: John Maura , Haselbeck Florian , Dass Rupashree , Malisi Christoph , Ricca Patrizia , Dreischer Christian , Schultheiss Sebastian J. , Grimm Dominik G.
Published: November 2022

Genomic selection is an integral tool for breeders to accurately select plants directly from genotype data leading to faster and more resource-efficient breeding programs. Several prediction methods have been established in the last few years. These range from classical linear mixed models to complex non-linear machine learning approaches, such as Support Vector Regression, and modern deep learning-based architectures. Many of these methods have been extensively evaluated on different crop species with varying outcomes. In this work, our aim is to systematically compare 12 different phenotype prediction models, including basic genomic selection methods to more advanced deep learning-based techniques. More importantly, we assess the performance of these models on simulated phenotype data as well as on real-world data from Arabidopsis thaliana and two breeding datasets from soy and corn. The synthetic phenotypic data allow us to analyze all prediction models and especially the selected markers under controlled and predefined settings. We show that Bayes B and linear regression models with sparsity constraints perform best under different simulation settings with respect to explained variance. Further, we can confirm results from other studies that there is no superiority of more complex neural network-based architectures for phenotype prediction compared to well-established methods. However, on real-world data, for which several prediction models yield comparable results with slight advantages for Elastic Net, this picture is less clear, suggesting that there is a lot of room for future research.
Effects of post-silking low temperature on the starch and protein metabolism, endogenous hormone contents, and quality of grains in waxy maize
Authors: Guo Jian , Qu Lingling , Wei Qi , Lu Dalei
Published: November 2022

Waxy maize has many excellent characteristics in food and nonfood industries. However, post-silking low temperature (LT) has severe limitations on its grain yield and quality. In this study, field and pot trials were conducted to investigate the effects of post-silking LT on the physiological, biochemical, and functional characteristics of two waxy maize grains. The field and pot trials were performed with sowing date and artificial climate chamber, respectively, for LT treatment from silking stage to maturity. Results in pot trial were used to explain and validate the findings in field trial. Compared with the ambient treatment, the LT treatment significantly reduced kernel weight during the grain filling stage (P < 0.05). LT treatment in both environments resulted in an average decrease in dry weight of SYN5 and YN7 at maturity by 36.6% and 42.8%, respectively. Enzymatic activities related to starch and protein biosynthesis decreased under the LT treatment during the filling stage, accompanied by a decrease in the accumulation amounts and contents of soluble sugar and starch, and a decrease in protein accumulation amount. Meanwhile, the contents of abscisic acid, indole-3-acetic acid, and gibberellin 3 in grains decreased under the LT treatment during the filling stage. Peak, trough, breakdown, final, and setback viscosities of grains decreased by LT. LT treatment decreased the gelatinization enthalpy of grains and increased the retrogradation percentage. In conclusion, post-silking LT stress altered the content of grain components by inhibiting the production of phytohormones and down-regulating the enzymatic activities involved in starch and protein metabolism, which resulted in the deterioration of grain pasting and thermal properties.
Creativity and (global, ethnic, host) cultural identifications: An examination in migrant and host national samples
Authors: Soler Pastor Elia , Bobowik Magdalena , Benet Martínez Verónica
Published: November 2022

We live in an era of unprecedented interconnectivity and challenges (e.g., climate change, pandemics) that require global mindsets and creative approaches. While research on global identification has increased in recent years, the question of whether it can facilitate creativity remains largely unexplored. Moreover, despite the evidence linking multicultural experiences and global identities, migrant populations have been overly underrepresented in this area of research. We examine the association between global culture identification and creativity in the Alternate Uses Test, across two different samples residing in Spain: a host national and majorly student sample (N = 326) and a culturally diverse immigrant sample (N = 122). Additionally, we test the predictive value of ethnic identification (in both samples) and host culture identification (in the immigrant sample). Regression analyses reveal that global culture identification positively predicts creativity among host national participants, and host culture identification predicts creativity among immigrant participants. Our results suggest that developing a cultural identity that transcends the one acquired through enculturation (i.e., global culture identification for the host national sample, host culture identification for the immigrant sample) has the potential of facilitating creative behavior.
Teachers’ demographic and occupational attributes predict feelings of hopelessness during the COVID-19 pandemic
Authors: Ghasemi Farshad
Published: November 2022

The outbreak of the COVID-19 pandemic has resulted in many emotional consequences for teachers, including feelings of isolation, loneliness, and hopelessness. However, evidence on the prevalence of hopelessness and the associated factors in teachers during the pandemic is limited. The purpose of this research was to examine the prevalence of hopelessness in public school teachers and identify risk factors associated with it. A sample of 168 teachers aged 25–49 years participated in the study by completing the Socio-Demographic Questionnaire, the Beck Hopelessness Scale (BHS), and the Multi-Dimensional Scale of Perceived Social Support (MSPSS). The results revealed a moderate level of experienced hopelessness in teachers. Hopelessness prevalence was also significantly different across teacher gender (males = 79%), age groups (>40 = 77%), socioeconomic status (poor socioeconomic status = 70%), educational level (high school teachers = 79%), professional experience (experienced teachers = 82%), and perceived social support (low perceived social support = 79%). The results of a logistic regression analysis confirmed the effects of these demographic and occupational attributes on hopelessness by explaining ~71% of the variance in hopelessness feelings. Higher odds ratios were associated with age, socioeconomic status, and perceived social support, signifying the prominence of these factors in predicting hopelessness. The study contributes to identifying and screening teachers at risk of hopelessness in public schools and recommends promoting collegial/superior support as well as a positive school climate as the protective factors against hopelessness.
A study on the correlation between work stressors and the coping styles of outpatients and emergency nurses in 29 pediatric specialty hospitals across China
Authors: Song Nan , Wang Chun-Li , Zhang Lin-Qi , Wang Xu-Mei
Published: November 2022

Objective

This study aimed to better understand the current situation involving work stressors and the coping styles of outpatient and emergency nurses in 29 pediatric specialty hospitals across China. The study analyzed this correlation to provide a reference for the occupational stress management of pediatric nurses.

Methods

From June to September 2020, 1,457 outpatient and emergency nurses in 29 pediatric specialty hospitals across China were selected as study participants, and a questionnaire survey was conducted using the Basic Information Questionnaire, the Chinese version of the Work Stressor Scale for Nurses, and the Simple Coping Style Scale.

Results

The assessed stress level of outpatient and emergency nurses in 29 tertiary pediatric specialty hospitals nationwide is lower than the results of the survey of the 2007 domestic norm, p < 0.05. The stressors related to nurses’ expectations, family conflicts, the nature of nursing work, patient factors, and workload were lower compared with the national norm (p < 0.05). The positive coping style score on the Simple Coping Style Scale for pediatric outpatient nurses was (36.66 ± 6.16), and work stressors were positively associated with negative coping styles (p < 0.01). Multiple regression analysis showed that the influencing factors of work stressors among pediatric outpatient and emergency nurses correlated with the authorized size, age, working years of nurses, work department, and negative coping styles.

Conclusion

Negative coping styles were present among pediatric outpatient and emergency nurses and were associated with work stressors. The influencing factors of stressors mainly correlated with the clinical establishment, age, years of employment as a nurse, work department, and negative coping styles.
The identity construction of Iranian English students learning translated L1 and L2 short stories: Aspiration for language investment or consumption?
Authors: Shahidzade Farangis , Mazdayasna Golnar
Published: November 2022

A large number of investigations have highlighted the importance of incorporating literary texts into English language teaching programs. Nevertheless, there are scarce studies on how short stories from L1 and L2 literature play a role in reconstructing learner identity in tertiary contexts. The present research study examines the identities of four non-native undergraduate students concerning aspirations for language investment or consumption. Data collection instruments were semi-structured interviews, open-ended questionnaires, and diary writings. The materials taught in the course consisted of three translated Persian and four target fictions related to loyalty and justice. The researchers used qualitative content analysis to explore the language learners’ self-images of initial, story-based, and prospective learning stages. Nearly all the participants regarded language learning as language consumption for pleasure in different stages except for the future aspiration stage; they did not aspire for personal economic benefits. The findings may enlighten curriculum designers in EFL contexts to incorporate literary texts from the native and target cultures into language education materials to provide opportunities for learners to experience diverse identities and meet individual preferences.
The directionality of anxiety and gaming disorder: An exploratory analysis of longitudinal data from an Australian youth population
Authors: Kim Seungyeon , Champion Katrina E. , Gardner Lauren A. , Teesson Maree , Newton Nicola C. , Gainsbury Sally M.
Published: November 2022

Gaming activities among adolescents have increased during the COVID-19 pandemic, bringing with it a growing concern for the potential harms of excessive gaming and its risk factors. Anxiety is frequently linked with gaming disorder, but studies investigating this association were mostly cross-sectional in design. Longitudinal studies that explore risk factors associated with gaming disorder are sparse and the trajectories of gaming disorder remain unclear. To address this paucity, the present study analyzed a large longitudinal dataset with a 12-month follow-up of 4,968 Australian adolescents (ages 13–14) during the pandemic. Logistic regression and multiple regression analyses were conducted to investigate the temporal relationships between anxiety, gaming frequency, the amount of money spent within video games, and gaming disorder. Prevalence rates for gaming disorder in adolescents aged 13 and 14 were 15 and 16%, respectively. The regression models indicated a bidirectional relationship between anxiety and gaming disorder symptoms, where higher levels of anxiety were associated with higher levels of gaming disorder 12 months later and vice versa. The study also found that the interaction between anxiety and higher gaming frequency could predict gaming disorder symptoms. Overall, the findings suggest that young adolescents may be more vulnerable to developing gaming disorder and highlight the importance of addressing the interactions between risk factors and gaming disorder in treatment approaches.
An analysis of patient motivation for seeking online treatment for binge eating disorder—A mixed methods study combining systematic text condensation with sentiment analysis
Authors: Holmberg Trine Theresa , Sainte-Marie Maxime , Jensen Esben Kjems , Linnet Jakob , Runge Eik , Lichtenstein Mia Beck , Tarp Kristine
Published: November 2022

Objective

Online treatment for binge eating disorder (BED) is an easily available option for treatment compared to most standard treatment procedures. However, little is known about how motivation types characterize this population and how these impact treatment adherence and effect in an online setting. Therefore, we aimed to investigate a sample of written motivation statements from BED patients, to learn more about how treatment and online treatment in particular, presents in this population.

Methods

Using self-determination theory in a mixed methods context, we investigated which types of motivation were prevalent in our sample, how this was connected with patient sentiment, and how these constructs influence treatment and adherence.

Results

Contrary to what most current literature suggests, we found that in our sample (n = 148), motivation type was not connected with treatment outcome. We did find a strong association between sentiment scores and motivation types, indicating the model is apt at detecting effects. We found that when comparing an adult and young adult population, they did not differ in motivation type and the treatment was equally effective in young adults and adults. In the sentiment scores there was a difference between sentiment score and adherence in the young adult group, as the more positive the young adults were, the less likely they were to complete the program.

Discussion

Because motivation type does not influence online treatment to the same degree as it would in face-to-face treatment it indicates that the typical barriers to treatment may be less crucial in an online setting. This should be considered during intake; as less motivated patients may be able to adhere better to online treatment, because the latter imposes fewer barriers of the kind that only strong motivation can overcome. The fact that motivation type and sentiment score of the written texts are strongly associated, indicate a potential for automated models to detect motivation based on sentiment.
Multidimensional analysis of job advertisements for medical record information managers
Authors: Dai Pingping , Zou Tongkang , Cheng Haiwei , Xin Zirui , Ouyang Wei , Peng Xiaoqing , Luo Aijing , Xie Wenzhao
Published: November 2022

Objective

The rapid growth of the medical industry has resulted in a tremendous increase in medical record data, which can be utilized for hospital management, aiding in diagnosis and treatment, medical research, and other purposes. For data management and analysis, medical institutions require more qualified medical record information managers. In light of this, we conducted an analysis of the qualifications, abilities, and job emphasis of medical record information managers in order to propose training recommendations.

Materials and methods

From online job posting sites, a sample of 241 job advertisements for medical record information management positions posted by Chinese healthcare institutions were collected. We conducted word frequency and keyword co-occurrence analysis to uncover overall demands at the macro level, and job analysis to investigate job-specific disparities at the micro level. Based on content analysis and job analysis, a competency framework was designed for medical record information managers.

Results

The most frequent keywords were “code,” “job experience,” and “coding certification,” according to the word frequency analysis. The competency framework for managers of medical record information is comprised of seven domains: essential knowledge, medical knowledge, computer expertise, problem-solving skills, leadership, innovation, and attitude and literacy. One of the fundamental skills required of medical record information managers is coordination and communication. Similarly, knowledge and skill requirements emphasize theoretical knowledge, managerial techniques, performance enhancement, and innovation development.

Conclusion

According to organization type and job differences, the most crucial feature of the job duties of medical record information managers is cross-fertilization. The findings can be utilized by various healthcare organizations for strategic talent planning, by the field of education for medical record information managers for qualification and education emphasis adjustment, and by job seekers to enhance their grasp of the profession and self-evaluation.
Acceptance of COVID-19 vaccine and associated factors among health care workers at public hospitals in Eastern Ethiopia using the health belief model
Authors: Getachew Tamirat , Lami Magarsa , Eyeberu Addis , Balis Bikila , Debella Adera , Eshetu Bajrond , Degefa Meron , Mesfin Sinetibeb , Negash Abraham , Bekele Habtamu , Turiye Getahun , Tamiru Dawit , Nigussie Kabtamu , Asfaw Henock , Dessie Yadeta , Alemu Addisu , Sertsu Addisu
Published: November 2022

Introduction

Acceptance of COVID-19 vaccination among Health Care Workers is mandatory to lessen and curve the spread of transmission of COVID-19. Even though the Health Belief Model is one of the most widely used models for understanding vaccination behavior against COVID-19 disease, COVID-19 vaccine acceptance among Health Care Workers in Ethiopia was not adequately explored by using the Health Belief Model domains.

Purpose

This study aimed to assess COVID-19 vaccine acceptance and associated factors among Health care workers in eastern, Ethiopia.

Methods

Institutional-based cross-sectional study design was used among 417 health care workers selected by a systematic random sampling method from June 1- 30/2021. The data were collected by face-to-face interviews using semi-structured questionnaires and analyzed using STATA version 14 statistical software. Multivariable binary logistic regression analysis with a 95% confidence interval was carried out to identify factors associated with willingness to COVID-19 vaccine acceptance and a statistical significance was declared at a P-value < 0.05.

Results

The willingness of health care workers to accept the COVID-19 vaccine was 35.6%. Age 30-39 (AOR = 4.16;95% CI: 2.51, 6.88), age ≥ 40 years (AOR = 3.29;95% CI: 1.47, 7.39), good attitude (AOR = 1.97; 95% CI: 1.00, 3.55), perceived susceptibility (AOR = 1.93; 95% CI: 1.12, 3.32), and perceived severity (AOR = 1.78; 95% CI: 1.03, 3.10) were factors significantly associated with Health Care Workers acceptance of COVID-19 vaccine.

Conclusion

The willingness to accept the COVID-19 vaccine among HCWs was low. Factors significantly associated with the willingness to accept the COVID-19 vaccine were age, good attitude, perceived susceptibility, and perceived severity of the disease. The low willingness of Health Care Workers to accept the COVID-19 vaccine was alarming and it needs more emphasis from the government in collaboration with other stakeholders to provide reliable information to avert misconceptions and rumors about the vaccine to improve the vaccine status of Health Care Workers to protect the communities.
Transmission of SARS-CoV-2 during indoor clubbing events: A clustered randomized, controlled, multicentre trial protocol
Authors: Goupil de Bouillé Jeanne , Luong Nguyen Liem Binh , Crépey Pascal , Garlantezec Ronan , Doré Véronique , Dumas Audrey , Ben Mechlia Mohamed , Tattevin Pierre , Gaudart Jean , Spire Bruno , Lert France , Yazdanpanah Yazdan , Delaugerre Constance , Noret Marion , Zeggagh Jeremy
Published: November 2022

Introduction

The SARS-CoV-2 pandemic led to the implementation of several non-pharmaceutical interventions (NPIs), from closings of bars and restaurants to curfews and lockdowns. Vaccination campaigns started hoping it could efficiently alleviate NPI. The primary objective of the “Indoor Transmission of COVID-19” (ITOC) study is to determine among a fully vaccinated population the relative risk of SARS-CoV-2 transmission during one indoor clubbing event. Secondary objectives are to assess the transmission of other respiratory viruses, risk exposure, and attitudes toward COVID-19 vaccination, health pass, and psychological impact of indoor club closing.

Methods and analysis

Four thousand four hundred healthy volunteers aged 18–49 years and fully vaccinated will be included in Paris region. The intervention is an 8-hour indoor clubbing event with no masks, no social distance, maximum room capacity, and ventilation. A reservation group of up to 10 people will recruit participants, who will be randomized 1:1 to either the experimental group (2,200 volunteers in two venues with capacities of 1,000 people each) or the control group (2,200 volunteers asked not to go to the club). All participants will provide a salivary sample on the day of the experiment and 7 days later. They also will answer several questionnaires. Virological analyses include polymerase chain reaction (PCR) of salivary samples and air of the venue, investigating SARS-CoV-2 and 18 respiratory viruses.

Ethics and dissemination

Ethical clearance was first obtained in France from the institutional review board (Comité de Protection des Personnes Ile de France VII - CPP), and the trial received clearance from the French National Agency for Medicines and Health Products (Agence National de Sécurité du Médicament - ANSM). The trial is supported and approved by The Agence Nationale Recherche sur le SIDA, les hépatites et maladies émergences (ANRS-MIE). Positive, negative, and inconclusive results will be published in peer-reviewed scientific journals.

Trial registration number

IDR-CB 2021-A01473-38. Clinicaltrial.gov, identifier: NCT05311865.
Initial adaptation of the OnTrack coordinated specialty care model in Chile: An application of the Dynamic Adaptation Process
Authors: Le PhuongThao D. , Choe Karen , Burrone María Soledad , Bello Iruma , Velasco Paola , Arratia Tamara , Tal Danielle , Mascayano Franco , Jorquera María José , Schilling Sara , Ramírez Jorge , Arancibia Diego , Fader Kim , Conover Sarah , Susser Ezra , Dixon Lisa , Alvarado Rubén , Yang Lawrence H. , Cabassa Leopoldo J.
Published: November 2022

Background

In 2005, Chile became the first country in Latin America to guarantee universal free access for the diagnosis and treatment of schizophrenia. A cluster randomized control trial utilizing the Dynamic Adaptation Process framework is underway to adapt and test the OnTrack coordinated specialty care model to provide recovery-oriented, person-centered care by a multidisciplinary team for individuals with first episode psychosis (FEP) in Chile.

Methods

A qualitative formative research study was conducted to inform the initial adaptation of the OnTrack Chile (OTCH) program. We conducted key informant interviews (n = 17) with various stakeholders (policymakers; directors/managers of community mental health centers; mental health professionals) and focus group discussions (n = 6) with individuals with FEP and caregivers (n = 35 focus group participants total). Data was analyzed using thematic analysis, organized by participants' perspectives on the benefits, barriers, and recommendations for the key principles, multidisciplinary team, psychosocial components, and the training and supervision model of OnTrack.

Results

Participants expressed enthusiasm and support for OnTrack's recovery-oriented and person-centered principles of care. While many participants lauded the emphasis on shared decision-making and family involvement, some reported reticence, citing that it is culturally normative for patients and families to adopt a passive role in treatment. Peer specialists, and the family psychoeducation and support and supported education and employment components were perceived as aspects that could encourage the promotion of personhood and autonomy development. However, implementation challenges, including the prevailing biomedical approach, professional hierarchy, and the lack of infrastructure, human, and financial resources necessitate some modifications to these aspects. Some mental health professionals further conveyed reservations regarding the perceived hierarchical structure of the supervision model.

Conclusion

OnTrack represents a shift from a biomedical model to a valued, aspirational, person-centered and culturally responsive model that focuses on recovery, shared decision-making and psychosocial care. With the appropriate governmental and agency-level provision of resources and modifications to some of the program components, particularly regarding the shared decision-making framework, peer specialist, family engagement, and the training supervision model, OTCH could be a transformative program for a more comprehensive, evidence-based care for individuals with FEP in Chile.
Identifying wild bee visitors of major crops in North America with notes on potential threats from agricultural practices
Authors: Rondeau Sabrina , Willis Chan D. Susan , Pindar Alana
Published: November 2022

Considering the critical importance of insect pollination to food security and documented declines in wild bee populations, it is imperative to develop effective conservation and management strategies that promote the health of wild bee communities associated with agroecosystems. Identifying wild bee visitors of crops, including crop-flower visitors and species that nest within cropping areas, may prove critical to this endeavor as optimal conservation strategies may differ among bee species and/or guilds, regions, and cropping systems. Although lists of bee species that are associated with North American crops are scattered throughout the literature, there is a need for a comprehensive compilation of those species by crop, region, and nesting guild. Here, we searched the literature to compile a list of wild bee species associated with 33 major crops in North America and assessed the overlap in bee species communities among crops and regions. Of the 739 crop-associated bee species retrieved, 405 species (54.8%) were ground nesters, and 438 species (59.3%) were identified as crop-flower visitors of at least one crop. Because of their nesting and foraging behavior, we argue that these species are more likely to be exposed to agricultural pesticide residues than bee species that do not nest in the ground or feed directly on crop flowers. We further compiled lists of wild bees that have been found to be associated with all of the four most surveyed perennial fruit crops (apple, blueberry, cranberry, strawberry) and all of the three most surveyed cucurbit crops (cucumber, Cucurbita, watermelon) in eastern North America. These lists of bee species can be used to focus attention on species needing better protection strategies within agroecosystems, especially for these two important North American crop groups and can also inform the development of multi-species pesticide risk assessment schemes.
Case report: A case report and literature review of extrapancreatic solid pseudopapillary neoplasm
Authors: Liu Hang , Xu Zhiquan , Wang Yaxu , Gu Haitao , Tang Yunhao , Wu Dabin , Wang Jijian , Zhang Jianbo
Published: November 2022

Background

Solid pseudopapillary neoplasm (SPN) is a rare tumor with low malignant potential, which typically occurs in the pancreas. Extrapancreatic SPN is also extremely rare worldwide.

Case presentation

We report a case of a 70-year-old woman hospitalized with abdominal pain and bloating. The patient did not have any underlying diseases, such as diabetes, coronary heart disease, or hypertension. More than 30 years ago, the patient underwent surgery for “ectopic pregnancy”. The patient had no family history of hereditary disease, nor did any immediate family members have a history of cancer. Laboratory tests showed that her hemoglobin and albumin levels were low and she had a high level of cancer antigen 125 (CA125). Enhanced computed tomography (CT) showed a large tumor in the abdomen and pelvis. The patient subsequently underwent surgery, and it was found that the tumor was attached to the terminal ileum. Pathological findings suggested that the tumor was an extrapancreatic SPN, with an ectopic pancreas found in the tumor tissue. The patient did not receive chemotherapy or radiotherapy after surgery. After 13 months of follow-up, the patient was admitted again with abdominal pain. CT showed tumor recurrence with extensive systemic metastases. The patient and her family refused reoperation and biopsy, and the patient was discharged after the abdominal pain and anemia resolved.

Conclusion

We report a rare case of extrapancreatic SPN of ileal origin, which could be the first report worldwide. It had aggressive biological features, with recurrence and metastasis 13 months after surgery. For extrapancreatic SPN, the risk of recurrence should be assessed, and for tumors suspected of malignant behavior, a longer follow-up after discharge may be needed. Although SPN generally has a good prognosis after surgery, there is no consensus on whether postoperative chemotherapy and other treatments are needed for patients with high recurrence risk.
The predictors and surgical outcomes of different distant metastases patterns in upper tract urothelial carcinoma: A SEER-based study
Authors: Hu Xuan-han , Miao Jia , Qian Lin , Zhang Da-hong , Wei Hai-bin
Published: November 2022

The purpose of this study was to investigate the predictors of metastatic patterns of upper tract urothelial carcinoma (UTUC) and to analyze the surgical outcomes of different metastatic patterns of UTUC. Data on patients with UTUC from 2010 to 2017 were retrieved from the Surveillance, Epidemiology, and End Results Program (SEER) database. Kaplan–Meier analysis was applied to compare the patients' survival distributions. Univariate and multivariate logistic regression was used to assess the specific predictors of site-specific metastases, while competitive risk regression was applied to estimate the predictors of cancer-specific mortality in patients with metastases. A total of 9,436 patients were enrolled from the SEER database, of which 1,255 patients had distant metastases. Lung metastasis (42.5%) was most common and patients with single distant lymph node metastasis had a better prognosis. Clinical N stage (N1, N2, N3) was the strongest predictors of the site specific metastatic sites. Renal pelvis carcinoma was more prone to develop lung metastases (OR = 1.67, P < 0.01). Resection of the primary tumor site is beneficial for the prognosis of patients with metastatic UTUC, whether local tumor resection (HR = 0.72, P < 0.01) or nephroureterectomy (HR = 0.64, P < 0.01). Patients with single distant lymph node metastasis have the greatest benefit in nephroureterectomy compared to other specific-site metastases (median survival 19 months vs. 8 months). An understanding of distant metastatic patterns and surgical outcomes in patients with UTUC is important in clinical settings and helpful in the design of personalized treatment protocols.
Liver tissue remodeling following ablation with irreversible electroporation in a porcine model
Authors: Monleón Eva , Lucía Óscar , Güemes Antonio , López-Alonso Borja , Arribas Dolores , Sarnago Héctor , Hernaez Alba , Burdío José Miguel , Junquera Concepción
Published: November 2022

Irreversible electroporation (IRE) is a method of non-thermal focal tissue ablation characterized by irreversibly permeabilizing the cell membranes while preserving the extracellular matrix. This study aimed to investigate tissue remodeling after IRE in a porcine model, especially focusing on the extracellular matrix and hepatic stellate cells. IRE ablation was performed on 11 female pigs at 2,000 V/cm electric field strength using a versatile high-voltage generator and 3 cm diameter parallel-plate electrodes. The treated lobes were removed during surgery at 1, 3, 7, 14, and 21 days after IRE. Tissue remodeling and regeneration were assessed by histopathology and immunohistochemistry. Throughout the treated area, IRE led to extensive necrosis with intact collagenous structures evident until day 1. From then on, the necrosis progressively diminished while reparative tissue gradually increased. During this process, the reticulin framework and the septal fibrillar collagen remained in the necrotic foci until they were invaded by the reparative tissue. The reparative tissue was characterized by a massive proliferation of myofibroblast-like cells accompanied by a complete disorganization of the extracellular matrix with the disappearance of hepatic architecture. Hepatic stellate cell markers were associated with the proliferation of myofibroblast-like cells and the reorganization of the extracellular matrix. Between 2 and 3 weeks after IRE, the lobular architecture was almost completely regenerated. The events described in the present study show that IRE may be a valid model to study the mechanisms underlying liver regeneration after extensive acute injury.
Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow
Authors: Fernandes Kamila Peruchi , Alfieri Amauri Alcindo , Darold Gabriela Molinari , Boabaid Fabiana Marques , Dall Agnol Alais Maria , Lunardi Michele
Published: November 2022

Bovine papillomavirus (BPV) infection can induce neoplastic lesions in both cutaneous and mucosal epithelia in cattle. This study describes the BPV types associated with proliferative lesions with diverse histopathological features present in the upper alimentary tract of a dairy cow suffering from chronic diarrhea from Midwestern Brazil. At autopsy, warts and plaques composed of multiple spherical nodules were observed in the esophageal mucosa, the areas surrounding and constricting the opening of the cardia, and the rumen pillars. One esophageal papillomatous proliferative lesion and a smooth-surfaced proliferative lesion located at the rumen entrance were evaluated by histopathological and molecular analyses. PCR amplification of partial fragments of the BPV L1 and E1 genes was performed followed by sequencing of the obtained amplicons. Upon histopathological evaluation, the esophageal lesion was classified as a squamous papilloma, whereas the other ruminal proliferative lesion consisted of a fibropapilloma. Direct sequencing of PCR products obtained from ruminal fibropapilloma DNA revealed the presence of BPV2. Sequencing of inserts from selected clones containing partial fragments of the BPV L1 and E1 genes revealed a mixed infection of BPV types 2 and 4 in the esophageal squamous papilloma. The findings reported in our investigation reinforce the association of BPV with benign lesions of the bovine alimentary tract in both single and mixed infections, as previously demonstrated to occur in a buffalo. In addition, this report represents the documentation of the occurrence of massive alimentary papillomatosis associated with BPV types 2 and 4 in cattle raised on lands without infestation by bracken fern in Midwestern Brazil.
MicroRNA and circular RNA profiling in the deposited fat tissue of Sunite sheep
Authors: He Xige , Wu Rihan , Yun Yueying , Qin Xia , Huang Yajuan , Chen Lu , Han Yunfei , Wu Jindi , Sha Lina , Borjigin Gerelt
Published: November 2022

As the most typical deposited fat, tail fat is an important energy reservoir for sheep adapted to harsh environments and plays an important role as a raw material in daily life. However, the regulatory mechanisms of microRNA (miRNA) and circular RNA (circRNA) in tail fat development remain unclear. In this study, we characterized the miRNA and circRNA expression profiles in the tail fat of sheep at the ages of 6, 18, and 30 months. We identified 219 differentially expressed (DE) miRNAs (including 12 novel miRNAs), which exhibited a major tendency to be downregulated, and 198 DE circRNAs, which exhibited a tendency to be upregulated. Target gene prediction analysis was performed for the DE miRNAs. Functional analysis revealed that their target genes were mainly involved in cellular interactions, while the host genes of DE circRNAs were implicated in lipid and fatty acid metabolism. Subsequently, we established a competing endogenous RNA (ceRNA) network based on the negative regulatory relationship between miRNAs and target genes. The network revealed that upregulated miRNAs play a leading role in the development of tail fat. Finally, the ceRNA relationship network with oar-miR-27a_R-1 and oar-miR-29a as the core was validated, suggesting possible involvement of these interactions in tail fat development. In summary, DE miRNAs were negatively correlated with DE circRNAs during sheep tail fat development. The multiple ceRNA regulatory network dominated by upregulated DE miRNAs may play a key role in this developmental process.
Hybrid InceptionV3-SVM-Based Approach for Human Posture Detection in Health Monitoring Systems
Authors: Ogundokun Roseline Oluwaseun , Maskeliūnas Rytis , Misra Sanjay , Damasevicius Robertas
Published: November 2022

Posture detection targets toward providing assessments for the monitoring of the health and welfare of humans have been of great interest to researchers from different disciplines. The use of computer vision systems for posture recognition might result in useful improvements in healthy aging and support for elderly people in their daily activities in the field of health care. Computer vision and pattern recognition communities are particularly interested in fall automated recognition. Human sensing and artificial intelligence have both paid great attention to human posture detection (HPD). The health status of elderly people can be remotely monitored using human posture detection, which can distinguish between positions such as standing, sitting, and walking. The most recent research identified posture using both deep learning (DL) and conventional machine learning (ML) classifiers. However, these techniques do not effectively identify the postures and overfits of the model overfits. Therefore, this study suggested a deep convolutional neural network (DCNN) framework to examine and classify human posture in health monitoring systems. This study proposes a feature selection technique, DCNN, and a machine learning technique to assess the previously mentioned problems. The InceptionV3 DCNN model is hybridized with SVM ML and its performance is compared. Furthermore, the performance of the proposed system is validated with other transfer learning (TL) techniques such as InceptionV3, DenseNet121, and ResNet50. This study uses the least absolute shrinkage and selection operator (LASSO)-based feature selection to enhance the feature vector. The study also used various techniques, such as data augmentation, dropout, and early stop, to overcome the problem of model overfitting. The performance of this DCNN framework is tested using benchmark Silhouettes of human posture and classification accuracy, loss, and AUC value of 95.42%, 0.01, and 99.35% are attained, respectively. Furthermore, the results of the proposed technology offer the most promising solution for indoor monitoring systems.
Exploring Factors Affecting Sustainable Consumption Behaviour
Authors: Dimitrova Teofana , Ilieva Iliana , Angelova Mina
Published: November 2022

Business development policies cover both marketing and sales functions, as they are so intertwined in most firms. Thus, managers should comply with the factors that influence sustainable consumption behaviour. The study aims to investigate the effect of environmental knowledge (EK), materialism (MAT), environmental influences (EI), the promotion of sustainable consumption (PSC), and sustainable consumption behaviour intention (SCBI) on sustainable consumption behaviour (SCB). Although many studies have examined sustainability issues for various groups of countries, activities in this critical field in Bulgaria are severely limited, and integrated research on the subject is non-existent. This paper focuses on the importance of investigating various factors that influence sustainable consumer behaviour. A total of 489 complete and usable responses were collected from participants from all regions of Bulgaria between May 2022 and July 2022. The partial least square-structural equation modelling (PLS-SEM) and SmartPLS 4 software were employed to test the hypothesised relationships. The results indicated that EK and MAT significantly affected SCBI. Additionally, the analysis revealed the statistically significant impact of EK, MAT, EI, PSC, and SCBI on SCB. Moreover, this study demonstrated that SCBI significantly mediated the relationships between EK and SCB and between MAT and SCB. Finally, the outcomes of the moderation analysis showed that age moderated the relationship between SCBI and SCB.
Fish Processing and Digestion Affect Parvalbumins Detectability in Gilthead Seabream and European Seabass
Authors: Schrama Denise , Raposo de Magalhães Cláudia , Cerqueira Marco , Carrilho Raquel , Revets Dominique , Kuehn Annette , Engrola Sofia , Rodrigues Pedro M.
Published: November 2022

Consumption of aquatic food, including fish, accounts for 17% of animal protein intake. However, fish consumption might also result in several side-effects such as sneezing, swelling and anaphylaxis in sensitized consumers. Fish allergy is an immune reaction to allergenic proteins in the fish muscle, for instance parvalbumin (PV), considered the major fish allergen. In this study, we characterize PV in two economically important fish species for southern European aquaculture, namely gilthead seabream and European seabass, to understand its stability during in vitro digestion and fish processing. This information is crucial for future studies on the allergenicity of processed fish products. PVs were extracted from fish muscles, identified by mass spectrometry (MS), and detected by sandwich enzyme-linked immunosorbent assay (ELISA) after simulated digestion and various food processing treatments. Secondary structures were determined by circular dichroism (CD) after purification by anion exchange and gel filtration chromatography. In both species, PVs presented as α-helical and β-sheet structures, at room temperature, were shown to unfold at boiling temperatures. In European seabass, PV detectability decreased during the simulated digestion and after 240 min (intestinal phase) no detection was observed, while steaming showed a decrease (p < 0.05) in PVs detectability in comparison to raw muscle samples, for both species. Additionally, freezing (−20 °C) for up to 12 months continued to reduce the detectability of PV in tested processing techniques. We concluded that PVs from both species are susceptible to digestion and processing techniques such as steaming and freezing. Our study obtained preliminary results for further research on the allergenic potential of PV after digestion and processing.