bluuebunny/crossref_metadata_2025 huggingface dataset:
Microsite Influence on Woody Plant Regeneration in a Pinus palustris Woodland Following Catastrophic Disturbance
Authors: Logan Alexandra T. , Goode Jonathan D. , Keellings David J. , Hart Justin L.
Published: May 2020

Information and material biological legacies that persist after catastrophic forest disturbance collectively constitute the ecological memory of the system and may strongly influence future stand development. Catastrophic disturbances often result in an influx of coarse woody debris (CWD), and this material legacy may provide beneficial microsites that affect successional and structural developmental pathways. We examined how microenvironmental characteristics influence the regeneration of woody plants in a subtropical woodland that experienced a large influx of CWD from a catastrophic wind disturbance. Specifically, we asked (1) what microenvironmental factors best explain woody plant density, richness, and height in the regeneration layer and (2) does woody plant density, richness, and height benefit from the large influx of CWD to a degree that competition dynamics and succession may be modified? Data were collected in a Pinus palustris woodland that had experienced an EF3 tornado and was subjected to a four-year prescribed fire rotation. We documented live woody plants <5 cm diameter at breast height, soil, and site characteristics and tested for differences in seedling and sapling density, species richness, and height in relation to CWD proximity. We used a random forest machine learning algorithm to examine the influence of microenvironmental conditions on the characteristics of woody plants in the regeneration layer. Woody plant density and species richness were not significantly different by proximity to CWD, but plants near CWD were slightly taller than plants away from CWD. The best predictors of woody plant density, richness, and height were abiotic site characteristics including slope gradient and azimuth, organic matter depth and weight, and soil water content. Results indicated that the regeneration of woody plants in this P. palustris woodland was not strongly influenced by the influx of CWD, but by other biological legacies such as existing root networks and soil characteristics. Our study highlights the need to consider ecological memory in forest management decision-making after catastrophic disturbance. Information and material legacies shape recovery patterns, but, depending on the system, some legacies will be more influential on successional and developmental pathways than others.
Intake of Radionuclides in the Trees of Fukushima Forests 3. Removal of Radiocesium from Stem Wood, Cryptomeria Japonica (L.f.) D. Don.
Authors: Seyama Tomoko , Arakawa Ryohei , Machida Shogo , Yoshida Sota , Maru Akihiko , Baba Kei’ichi , Kobayashi Yoshinori , Kaida Rumi , Taji Teruaki , Sakata Yoichi , Iijima Tomoaki , Hayashi Takahisa
Published: May 2020

Nuclear power plant accidents have dispersed radiocesium into the atmosphere to contaminate trees with no turnover in heartwood, as occurred in Fukushima, and as has persisted for over 30 years around Chernobyl. Here we employ the ponding method, in which radiocesium can be flushed out from the cross-cut edges of Japanese cedar, Cryptomeria japonica (L.f.) D. Don., stem with water due to xyloglucan degradation in tracheids. Furthermore, lab-scale ponding experiments have shown that a non-detectable level of radiocesium has been observed not only in the pool water used for 575 days but also in the water containing recombinant xyloglucanase. This traditional technology is now a new biotechnology.
Effects of Hormones and Epigenetic Regulation on the Callus and Adventitious Bud Induction of Fraxinus mandshurica Rupr.
Authors: Yu Lei , Li Xinyu , Tian Hongmei , Liu Hualing , Xiao Ying , Liang Nansong , Zhao Xingtang , Zhan Yaguang
Published: May 2020

Fraxinus mandshurica Rupr. (hereafter “F. mandshurica”) is known as one of northeast China′s important, valuable hardwood timber species. However, tissue culture and micropropagation of the species are difficult and have low efficiency, limiting asexual propagation. In this manuscript, stem explants were utilized to establish an effective regeneration system through adventitious bud organogenesis. The factors influencing callus regeneration in vitro were determined, and callus regeneration technology was established. The mechanism of adventitious bud formation was analyzed. Thidiazuron (TDZ) played a crucial role in the formation of adventitious buds. Elevated concentrations of TDZ were beneficial to callus induction and low concentrations of 6-benzyladenine (BA) led to loose state callus formation. The order of callus induction rates for different explants was stem cotyledon (100%) > segment (98.54%) > hypocotyl (92.56%) > root (50.71%). The effects of exogenous addition of 6-BA and TDZ on the endogenous hormone content of plants during the regeneration of adventitious buds were also assessed, as well as the expression characteristics of genes related to the regeneration pathway. The comprehensive analysis results showed that the suitable medium for callus induction and adventitious bud differentiation was c12 medium (MSB5 + 30 g/L sucrose + 7 g/L Agar + 5 mg/L 6-BA + 8 mg/L TDZ + 2 mg/L glycine + 0.1 mg/L IBA + 5% coconut water). The induction rates of callus and adventitious buds were 99.15% and 33.33%. The addition of 2.4 mg/L of the DNA demethylation reagent 5-azacytidine (5-aza) and 0.15 mg/L of the histone deacetylase inhibitor trichostatin A (TSA) increased the rates of adventitious bud induction by 17.78% over the control. This further laid the foundation for large-scale cultivation of excellent varieties and genetic transformation techniques.
Roads Impact Tree and Shrub Productivity in Adjacent Boreal Peatlands
Authors: Saraswati Saraswati , Bhusal Yubraj , Trant Andrew J. , Strack Maria
Published: May 2020

Peatlands in the western boreal plains of Canada are important ecosystems as they store over two percent of global terrestrial carbon. However, in recent decades, many of these peatlands have been fragmented by access roads constructed for resource extraction and transportation, challenging their carbon storage potential. To investigate how roads have been impacting tree and shrub growth and productivity in these peatlands, this study was conducted in a forested bog and woody fen in Carmon Creek, Alberta, Canada. In 2017, vegetation surveys were conducted along 20 m transects that extended on both sides of the road with 4 m2 circular plots at 2, 6 and 20 m distance from the road and were followed by disc or core collection from woody stems. Within 20 m of the road at the bog site, we observed a shift towards significantly larger radial growth of trees in the downstream areas (t = 3.23, p = 0.006) where water table position was deeper, while at the fen site, radial growth of tall shrubs had little response to the road. Combining the effects of direct tree clearing and hydrology induced shifts in growth, aboveground net primary productivity (NPPag) post-road construction was reduced significantly in areas where vegetation was cleared during the road construction (i.e., upstream areas of the bog: t = 5.21, p < 0.0001 and downstream areas of the fen: t = 2.64, p = 0.07). Substantially lower NPPag around the road construction areas compared to reference areas shows tremendous loss of carbon sink potential of trees and shrubs after road construction through peatlands. Altogether, roads constructed through peatlands perpendicular to the water flow may shift long-term carbon sinks into sources of carbon, at least for the initial few years following road construction.
The Effect of Topography on Long-Term Spontaneous Development of Soil and Woody Cover on Graded and Untreated Overburden
Authors: Vicentini Fabio , Hendrychova Markéta , Tajovský Karel , Pižl Václav , Frouz Jan
Published: May 2020

We studied the development of soil and soil fauna, as well as selected vegetation parameters important for litter input into soil, in two chronosequences of postmining sites after opencast lignite mining near the town of Most (Czechia). Both chronosequences did not have tree planting. On the first chronosequence, no leveling or any other measures had taken place after heaping, and the site kept the wavelike appearance created by the dumping process. The second chronosequence was formed by sites levelled by earthmoving machinery and in which shallow topsoil layers were spread. Both chronosequences were about 30 years old, and consisted of 8 and 11 sites for wavelike and levelled sites, respectively. In addition, samples were taken from a birch site outside of the heaps. Results showed differences in the development of tree and herb layers in both chronosequences. In the levelled sites, herb and tree cover increased after levelling, and woody cover developed much slower. In ungraded sites, woody cover developed faster, and the herb layer slower. Soil chemistry showed a similar pattern in both sites over time, characterized by a decrease in pH and sodium ion content. Soil macrofauna in levelled sites showed random oscillations, while it gradually increased with time in wavelike sites. The study suggested that site levelling alters long-term ecosystem development in postmining sites. Wavelike sites tend to develop towards pioneer forests, while leveled sites tend to be covered by grass and herb vegetation.
Spruce Galactoglucomannan-Stabilized Emulsions Enhance Bioaccessibility of Bioactive Compounds
Authors: Zhao Hongbo , Mikkonen Kirsi S. , Kilpeläinen Petri O. , Lehtonen Mari I.
Published: May 2020

The increasing public awareness of health and sustainability has prompted the development of functional foods rich in health-promoting ingredients. Processing technologies and sustainable multifunctional ingredients are needed for structuring these formulations. Spruce galactoglucomannan (GGM), the main hemicelluloses in softwood cell walls, are an abundantly available, emerging sustainable food hydrocolloid that have the ability to efficiently emulsify and stabilize oil-in-water emulsions. In this study, we illustrate how this lignocellulosic stabilizer affects the digestion of polyunsaturated fatty acids (PUFAs) in vitro. A 100% decrease in the initial TAG content was observed during the in vitro digestion, suggesting that complete hydrolysis of the TAGs was achieved by the digestive enzymes. Besides, no release of mono-, di-, and oligosaccharides or phenolic compounds from GGM was detected. Our results demonstrate that the GGM-stabilized emulsion could potentially deliver lipophilic bioactive ingredients and enhance their bioaccessibility. In addition, this bio-stabilizer itself would remain stable in the upper gastrointestinal track and serve as a prebiotic for gut microbiota. We anticipate GGM to complement or even replace many of the conventional carriers of bioactive components in future health care products and functional foods.
Heterogeneous Fenton Degradation of Patulin in Apple Juice Using Carbon-Encapsulated Nano Zero-Valent Iron (CE-nZVI)
Authors: Silwana Notemba , Calderón Blanca , Ntwampe Seteno Karabo Obed , Fullana Andrés
Published: May 2020

Patulin (PAT), a mycotoxin found mainly in matured apples, is produced by different species of fungi, mainly Penicillium expansum, and is found in various fruits and vegetables used to produce juice. Little focus has been placed on nano-technological methods for the mitigation of this problem. In this work, carbon-encapsulated nano-zero valent iron (CE-nZVI) particles were synthesized and used as heterogeneous Fenton agents for the degradation of PAT in apple juice. The particles were found to have a spherical shape with a diameter of 130 ± 50 nm. In a heterogeneous Fenton degradation (involving CE-nZVI) process, a concentration of 0.05 g/L CE-nZVI with 0.5 mM H2O2 was used. Since the Fenton oxidation process is pH-dependent, placebo degradation was observed at varying pH conditions with an average percentage of PAT degradation of 27.8%, 87.0%, 98.0%, and 99.75% at pH 6, 5, 4.5, and 3.5 respectively, between 1 min to 4 h in a water matrix. In a juice matrix, at the regular pH of juice (3.6), percentage PAT degradation of 72% and 89% was obtained after a 2-h treatment using heterogeneous Fenton oxidation (CE-nZVI/H2O2) systems, using 0.5 mM H2O2 and 1 mM H2O2, respectively.
Covid-19 and the Subsequent Lockdown Modified Dietary Habits of Almost Half the Population in an Italian Sample
Authors: Scarmozzino Federico , Visioli Francesco
Published: May 2020

The Covid-19 pandemic led to lockdowns in several parts of the world and, hence, changed some daily habits, including social interactions, the ability to perform sports, and—possibly—diet. The Italian government established and promulgated lockdown policies on 9 March 2020. We aim at assessing the effects of Covid-19-induced confinement policies on self-reported food consumption of self-selected Italians by means of a questionnaire that was created and diffused by the Internet. Nearly half, i.e., 49.6% of responders did not substantially modify their diet during the lockdown; however, 46.1% of them reported that they were eating more during confinement, and 19.5% gained weight. In particular, we report an increase in “comfort food” consumption, notably chocolate, ice-cream, and desserts (42.5%) and salty snacks (23.5%). In addition, 42.7% percent of this cohort attributed this increase to higher anxiety levels. Related to this, 36.8% of responders reported a decrease in alcohol consumption, even though 10.1% of them reported an increase. Interestingly, 21.2% of responders increased their consumption of fresh fruit and vegetables. Only 33.5% of those who declared decreased consumption attributed this change of diet to lower availability and ease of purchasing such items. Equally interesting, over half of responders, i.e., 56.2%, admitted that fruit and vegetables did not appeal to them while in lockdown. Purchases of ready-made meals were reduced by nearly 50%. Future large-scale similar studies should be undertaken worldwide and will help public health authorities shape their reactions to future, unavoidable pandemics.
Yield and Quality Characteristics of Brassica Microgreens as Affected by the NH4:NO3 Molar Ratio and Strength of the Nutrient Solution
Authors: Palmitessa Onofrio Davide , Renna Massimiliano , Crupi Pasquale , Lovece Angelo , Corbo Filomena , Santamaria Pietro
Published: May 2020

Microgreens are gaining more and more interest, but little information is available on the effects of the chemical composition of the nutrient solution on the microgreen yield. In this study, three Brassica genotypes (B. oleracea var. italica, B. oleracea var. botrytis, and Brassica rapa L. subsp. sylvestris L. Janch. var. esculenta Hort) were fertigated with three modified strength Hoagland nutrient solutions (1/2, 1/4, and 1/8 strength) or with three modified half-strength Hoagland nutrient solutions with three different NH4:NO3 molar ratios (5:95, 15:85, and 25:75). Microgreen yields and content of inorganic ions, dietary fiber, proteins, α-tocopherol, and β-carotene were evaluated. Micro cauliflower showed the highest yield, as well as a higher content of mineral elements and α-tocopherol (10.4 mg 100 g−1 fresh weight (FW)) than other genotypes. The use of nutrient solution at half strength gave both a high yield (0.23 g cm−2) and a desirable seedling height. By changing the NH4:NO3 molar ratio in the nutrient solution, no differences were found on yield and growing parameters, although the highest β-carotene content (6.3 mg 100 g−1 FW) was found by using a NH4:NO3 molar ratio of 25:75. The lowest nitrate content (on average 6.8 g 100 g−1 dry weight) was found in micro broccoli and micro broccoli raab by using a nutrient solution with NH4:NO3 molar ratios of 25:75 and 5:95, respectively. Micro cauliflower fertigated with a NH4:NO3 molar ratio of 25:75 showed the highest dry matter (9.8 g 100 g−1 FW) and protein content (4.2 g 100 g−1 FW).
Emulsifying and Anti-Oxidative Properties of Proteins Extracted from Industrially Cold-Pressed Rapeseed Press-Cake
Authors: Östbring Karolina , Nilsson Kajsa , Ahlström Cecilia , Fridolfsson Anna , Rayner Marilyn
Published: May 2020

One of the functional proteins in rapeseed—the amphiphilic protein oleosin—could be used to stabilize emulsions. The objectives of this study were to extract oleosins from cold-pressed rapeseed press-cake, optimize the extraction process, and investigate their emulsifying and anti-oxidative capacity. The proteins were recovered from industrially cold-pressed rapeseed press-cake at different alkali pHs. Emulsifying properties and oxidation rates were assessed. Oleosin extracted at pH 9 stabilized smaller emulsion droplets than oleosin extracted at pH 12, although the protein yield was higher at pH 12. Emulsions were formulated from flaxseed oil and corn oil and were stabilized by oleosin, bovine serum albumin, de-oiled lecithin and Tween 20 h and the emulsions were stored in accelerated conditions (30 °C) for 12 days. Oleosin stabilized emulsions to the same extent as commercial food-grade emulsifiers. Flaxseed oil emulsions stabilized by oleosin had a significantly lower concentration of malondialdehyde (MDA) which indicates a lower oxidation rate compared to BSA, de-oiled lecithin and Tween 20. For corn oil emulsions, oleosin and BSA had a similar capacity to delay oxidation and were significantly more efficient compared to de-oiled lecithin and Tween 20. Rapeseed oleosin recovered from cold-pressed rapeseed press-cake could be a suitable natural emulsifier with anti-oxidation properties.
Applicability of Confocal Raman Microscopy to Observe Microstructural Modifications of Cream Cheeses as Influenced by Freezing
Authors: Alinovi Marcello , Mucchetti Germano , Andersen Ulf , Rovers Tijs A. M. , Mikkelsen Betina , Wiking Lars , Corredig Milena
Published: May 2020

Confocal Raman microscopy is a promising technique to derive information about microstructure, with minimal sample disruption. Raman emission bands are highly specific to molecular structure and with Raman spectroscopy it is thus possible to observe different classes of molecules in situ, in complex food matrices, without employing fluorescent dyes. In this work confocal Raman microscopy was employed to observe microstructural changes occurring after freezing and thawing in high-moisture cheeses, and the observations were compared to those obtained with confocal laser scanning microscopy. Two commercially available cream cheese products were imaged with both microscopy techniques. The lower resolution (1 µm/pixel) of confocal Raman microscopy prevented the observation of particles smaller than 1 µm that may be part of the structure (e.g., sugars). With confocal Raman microscopy it was possible to identify and map the large water domains formed during freezing and thawing in high-moisture cream cheese. The results were supported also by low resolution NMR analysis. NMR and Raman microscopy are complementary techniques that can be employed to distinguish between the two different commercial formulations, and different destabilization levels.
Sequence Analysis and FISH Mapping of Four Satellite DNA Families among Cervidae
Authors: Vozdova Miluse , Kubickova Svatava , Cernohorska Halina , Fröhlich Jan , Martínková Natália , Rubes Jiri
Published: May 2020

Centromeric and pericentromeric chromosome regions are occupied by satellite DNA. Satellite DNAs play essential roles in chromosome segregation, and, thanks to their extensive sequence variability, to some extent, they can also be used as phylogenetic markers. In this paper, we isolated and sequenced satellite DNA I-IV in 11 species of Cervidae. The obtained satellite DNA sequences and their chromosomal distribution were compared among the analysed representatives of cervid subfamilies Cervinae and Capreolinae. Only satI and satII sequences are probably present in all analysed species with high abundance. On the other hand, fluorescence in situ hybridisation (FISH) with satIII and satIV probes showed signals only in a part of the analysed species, indicating interspecies copy number variations. Several indices, including FISH patterns, the high guanine and cytosine (GC) content, and the presence of centromere protein B (CENP-B) binding motif, suggest that the satII DNA may represent the most important satellite DNA family that might be involved in the centromeric function in Cervidae. The absence or low intensity of satellite DNA FISH signals on biarmed chromosomes probably reflects the evolutionary reduction of heterochromatin following the formation of chromosome fusions. The phylogenetic trees constructed on the basis of the satellite I-IV DNA relationships generally support the present cervid taxonomy.
Association Analysis and Meta-Analysis of Multi-Allelic Variants for Large-Scale Sequence Data
Authors: Jiang Yu , Chen Sai , Wang Xingyan , Liu Mengzhen , Iacono William G. , Hewitt John K. , Hokanson John E. , Krauter Kenneth , Laakso Markku , Li Kevin W. , Lutz Sharon M. , McGue Matthew , Pandit Anita , Zajac Gregory J.M. , Boehnke Michael , Abecasis Goncalo R. , Vrieze Scott I. , Jiang Bibo , Zhan Xiaowei , Liu Dajiang J.
Published: May 2020

There is great interest in understanding the impact of rare variants in human diseases using large sequence datasets. In deep sequence datasets of >10,000 samples, ~10% of the variant sites are observed to be multi-allelic. Many of the multi-allelic variants have been shown to be functional and disease-relevant. Proper analysis of multi-allelic variants is critical to the success of a sequencing study, but existing methods do not properly handle multi-allelic variants and can produce highly misleading association results. We discuss practical issues and methods to encode multi-allelic sites, conduct single-variant and gene-level association analyses, and perform meta-analysis for multi-allelic variants. We evaluated these methods through extensive simulations and the study of a large meta-analysis of ~18,000 samples on the cigarettes-per-day phenotype. We showed that our joint modeling approach provided an unbiased estimate of genetic effects, greatly improved the power of single-variant association tests among methods that can properly estimate allele effects, and enhanced gene-level tests over existing approaches. Software packages implementing these methods are available online.
Integrated Geodetic and Hydrographic Measurements of the Yacht Port for Nautical Charts and Dynamic Spatial Presentation
Authors: Makar Artur , Specht Cezary , Specht Mariusz , Dąbrowski Paweł , Szafran Maciej
Published: May 2020

The use of geodetic and hydrographic systems, methods and measuring devices to obtain geospatial data enables integrated modeling of 3D space and related objects. Factors such as the seafloor relief of the marina and land infrastructure need to be considered to ensure safe stay of vessels in the marina. The article presents the results of bathymetric and geodetic measurements of the yacht port of the National Sailing Centre in Gdańsk. They were made with the use of several measuring methods such as a hydrographic unmanned surface vessel, GNSS positioning, enabling geodetic inventory of hydrotechnical structures and port infrastructure, as well as laser scanning that resulted in three-dimensional presentation of land objects. As a result of research and analysis, a two-dimensional bathymetric chart of the yacht port was made. Thanks to the integration of geodetic and hydrographic data, three-dimensional visualization of the marina area was obtained. The charts can be used to ensure ship navigation safety when maneuvering in port and mooring at quays.
The Conceptual and Methodological Mayhem of “Screen Time”
Authors: K. Kaye Linda , Orben Amy , A. Ellis David , C. Hunter Simon , Houghton Stephen
Published: May 2020

Debates concerning the impacts of screen time are widespread. Existing research presents mixed findings, and lacks longitudinal evidence for any causal or long-term effects. We present a critical account of the current shortcomings of the screen time literature. These include poor conceptualisation, the use of non-standardised measures that are predominantly self-report, and issues with measuring screen time over time and context. Based on these issues, we make a series of recommendations as a basis for furthering academic and public debate. These include drawing on a user-focused approach in order to seek the various affordances gained from “screen use”. Within this, we can better understand the way in which these vary across time and context, and make distinction between objective measures of “screen time” compared to those more subjective experiences of uses or affordances, and the differential impacts these may bring.
An Exploration of a Synthetic Construction Land Use Quality Evaluation Based on Economic-Social-Ecological Coupling Perspective: A Case Study in Major Chinese Cities
Authors: Cui Xufeng , Yang Sheng , Zhang Guanghong , Liang Bin , Li Fei
Published: May 2020

Recently, with the rapid increase of urban population and industrial agglomeration, the price of construction land has increased, and construction land has become increasingly scarce. Therefore, how to improve the construction land use quality (CLUQ) becomes more and more important. The purpose of the study is to evaluate CLUQ in China’s major cities and to analyze the dominant obstacle factors for quality improvement in order to provide policy advice for construction land management. This study adapts the data from 2014 to 2016 and constructs the evaluation framework of CLUQ involving economic quality, social quality, and ecological quality of construction land to evaluate and analyze CLUQ with the synthetic evaluation model, coupling evaluation model, and obstacle diagnosis model (ECO model). This study shows that the synthetic CLUQ of 23 cities out of 36 major cities in China shows a general increasing state. The economic quality of 26 cities out of 36 major cities in China has increased, while the social and ecological quality of 20 out of 36 major cities in China has decreased. In terms of spatial characteristics, the synthetic quality in the east and southwest of China is relatively high; in terms of spatial trend, the synthetic quality in longitude increases from west to east, and it shows an inverted U-shaped state in latitude. Moreover, economic development is the main obstacle factor for the improvement of CLUQ in Hohhot, Lanzhou, Urumqi, and Changchun. Social development results in the CLUQ lagging in Beijing, Guiyang, Shanghai, Xining, and Chongqing. Ecological development has a negative impact in that of Harbin, Qingdao, and Wuhan. Furthermore. The improvement of CLUQ lies in the coupling and coordinated development of economic, social, and ecological quality. For those with a low coupling degree, the targeted suggestions are given for different types based on city’s quadrant distribution.
Potential Benefits and Harms of Novel Antidiabetic Drugs During COVID-19 Crisis
Authors: Mirabelli Maria , Chiefari Eusebio , Puccio Luigi , Foti Daniela Patrizia , Brunetti Antonio
Published: May 2020

Patients with diabetes have been reported to have enhanced susceptibility to severe or fatal COVID-19 infections, including a high risk of being admitted to intensive care units with respiratory failure and septic complications. Given the global prevalence of diabetes, affecting over 450 million people worldwide and still on the rise, the emerging COVID-19 crisis poses a serious threat to an extremely large vulnerable population. However, the broad heterogeneity and complexity of this dysmetabolic condition, with reference to etiologic mechanisms, degree of glycemic derangement and comorbid associations, along with the extensive sexual dimorphism in immune responses, can hamper any patient generalization. Even more relevant, and irrespective of glucose-lowering activities, DPP4 inhibitors and GLP1 receptor agonists may have a favorable impact on the modulation of viral entry and overproduction of inflammatory cytokines during COVID-19 infection, although current evidence is limited and not univocal. Conversely, SGLT2 inhibitors may increase the likelihood of COVID-19-related ketoacidosis decompensation among patients with severe insulin deficiency. Mindful of their widespread popularity in the management of diabetes, addressing potential benefits and harms of novel antidiabetic drugs to clinical prognosis at the time of a COVID-19 pandemic deserves careful consideration.
Italian Public Health Response to the COVID-19 Pandemic: Case Report from the Field, Insights and Challenges for the Department of Prevention
Authors: Torri Emanuele , Sbrogiò Luca Gino , Di Rosa Enrico , Cinquetti Sandro , Francia Fausto , Ferro Antonio
Published: May 2020

The coronavirus disease (COVID-19) outbreak is rapidly progressing globally, and Italy, as one of the main pandemic hotspots, may provide some hard lessons for other countries. In this paper, we summarize the current organizational capacity and provide a pragmatic and narrative account of strategies and activities implemented by the Department of Prevention (Dipartimento di Prevenzione)—the regional entity of the Local Health Authority of the Italian National Health Service in charge of public health—since the beginning of the outbreak. We conduct a preliminary analysis of general strengths, weaknesses, opportunities, and threats (SWOT) of the response strategies from a local perspective. Furthermore, we provide firsthand insights on future directions and priorities to manage this unprecedented pandemic. Our case report gives a qualitative view of the healthcare response, based on the experience of frontline professionals, with the aim to generate hypotheses about factors which may promote or hinder the prevention and management of a pandemic locally. We highlight the importance of a public health approach for responding to COVID-19 and reshaping healthcare systems.
Valproic Acid Impacts the Growth of Growth Plate Chondrocytes
Authors: Fan Hueng-Chuen , Wang Shih-Yu , Peng Yi-Jen , Lee Herng-Sheng
Published: May 2020

A range of bone abnormalities including short stature have been reported to be associated with the use of antiepileptic drugs (AEDs) in children. Exactly how AEDs impact skeletal growth, however, is not clear. In the present study, rat growth plate chondrocytes were cultured to study the effects of AEDs, including valproic acid (VPA), oxcarbazepine (OXA), levetiracetam (LEV), lamotrigine (LTG), and topiramate (TPM) on the skeletal growth. VPA markedly reduced the number of chondrocytes by apoptosiswhile other AEDs had no effect. The apoptosis associated noncleaved and cleaved caspase 3, and caspases were increased by exposure to VPA, which up-regulated cyclooxygenase 2 (COX-2) mRNA and protein levels likely through histone acetylation. The COX-2 inhibitor NS-398 attenuated the effects of VPA up-regulating COX-2 expression and decreased VPA-induced caspase 3 expression. The use of VPA in children should be closely monitored or replaced, where appropriate, by AEDs which do not apparently affect the growth plate chondrocytes.
Association Between Gout and Injury Risk: A National Retrospective Cohort Study
Authors: Ou Shih-Hsiang , Chou Chu-Lin , Lin Chia-Wei , Chien Wu-Chien , Fang Te-Chao , Lu Kuo-Cheng , Chen Jin-Shuen
Published: May 2020

The association between gout and injury remains unclear. This study aimed to investigate the injury risk in patients with gout. Using the Longitudinal Health Insurance Database (LHID) from 2000 to 2010 in Taiwan, patients with gout (group CFG) and those without gout (group C) were enrolled for further analysis. The CFG group was separated into two subgroups (with and without medication) to determine whether the risk of injury was reduced with drug intervention. The follow-up period was defined as the time from the initial diagnosis of gout to the date of injury. A total of 257,442 individuals were enrolled in this study, with 85,814 people in group CFG and 171,628 people in group C. Using Cox regression analysis, group CFG showed a significant increase in the risk of injury. Traffic injuries, poisoning, falls, crushing/cutting/piercing injury, and suicides were prominent among these injuries. Furthermore, when urate-lowing drugs were used to treat the CFG group, there were no significant differences in the occurrence of injury. Patients with gout had an increased risk of injury overall, and drug intervention did not lower the risk of injury in these patients.
The Benefits of Physical Exercise on Mental Disorders and Quality of Life in Substance Use Disorders Patients. Systematic Review and Meta-Analysis
Authors: Giménez-Meseguer Jorge , Tortosa-Martínez Juan , Cortell-Tormo Juan
Published: May 2020

Physical exercise seems to have a promising effect on numerous variables related to the recovery of drug-dependent patients. However, some contradictions are found in the literature. The aim of this study was to perform a systematic review and meta-analysis in order to identify the effect of physical exercise on mental disorders, quality of life, abstinence, and craving, and make a comparison of the effect of exercise depending on the type of program. A search for articles was conducted using PubMed, Web of Science, and Scopus databases. Studies were selected that measured the acute effects or long-term effect (≥2 weeks) of exercise in patients who met criteria for alcohol use disorders or substance use disorders. A total of 59 studies were included. An effect of exercise on quality of life and mental disorders was identified. Subgroup analysis revealed an effect of exercise on stress (SMD = 1.11 (CI: 0.31, 1.91); z = 2.73; p = 0.006), anxiety (SMD = 0.50 (CI: 0.16, 0.84); z = 2.88; p = 0.004) and depression (SMD = 0.63 (CI: 0.34, 0.92); z = 4.31; p < 0.0001), and an effect of exercise on the eight variables included in the SF36 test. The results also showed a trend towards a positive effect on craving (SMD = 0.89 (CI: −0.05, 1.82); z = 1.85, p = 0.06). Body–mind activities and programs based on improving physical conditions produced similar results in mental disorders and quality of life. Available evidence indicates that physical exercise, both body–mind and physical fitness programs, can be effective in improving mental disorders, craving, and quality of life in drug-dependent patients.
The Psychometric Characteristic of the Taekwondo Electronic Protector Cognition Scale: The Application of the Rasch Model
Authors: Cho Eun-Hyung , Jang Chang-Yong , Kwak Yi-Sub , Kim Eung-Joon
Published: May 2020

This research was to investigate the psychometric characteristics of the electronic protector cognition scale by the infit and outfit of taekwondo athletes. Participants were 216 athletes (male = 109; female = 117) from 19 countries competed at the 19th Taekwondo World Championships. The electronic protector cognition scale consisting of 24-item with four subscales was utilized. The electronic protector cognition scale used a five-point Likert grading with 1 (not at all) to 5 (very likely). Analysis using IBM SPSS STATISTICS version 23 (IBM SPSS, Inc., Chicago, IL, USA) was conducted for the 226 data sets collected. WINSTEPS 3.74 (Linacre, 2015) was used for calculating subject reliability, item goodness-of-fit, scale propriety, and item level of difficulty, in order to apply the item response theory to the psychometric characteristics of electronic protectors. The research results showed that it was suitable for subject infit/outfit in taekwondo electronic protector cognition scale as 1.00~1.01 and the input/output of taekwondo electronic protector cognition scale as 1.00~1.01. Secondly, five-point scales were reviewed to be suitable for scale propriety, resulting from stage index judgment. Thirdly, 8 items showed problems in item goodness-of-fit. Finally, scale propriety was reported to be suitable considering the ability distribution of taekwondo players and the level of scale difficulty.
Organic Air Quality Markers of Indoor and Outdoor PM2.5 Aerosols in Primary Schools from Barcelona
Authors: van Drooge Barend L. , Rivas Ioar , Querol Xavier , Sunyer Jordi , Grimalt Joan O.
Published: May 2020

Airborne particulate matter with an aerodynamic diameter smaller than 2.5 µg, PM2.5 was regularly sampled in classrooms (indoor) and playgrounds (outdoor) of primary schools from Barcelona. Three of these schools were located downtown and three in the periphery, representing areas with high and low traffic intensities. These aerosols were analyzed for organic molecular tracers and polycyclic aromatic hydrocarbons (PAHs) to identify the main sources of these airborne particles and evaluate the air quality in the urban location of the schools. Traffic emissions were the main contributors of PAHs to the atmospheres in all schools, with higher average concentrations in those located downtown (1800–2700 pg/m3) than in the periphery (760–1000 pg/m3). The similarity of the indoor and outdoor concentrations of the PAH is consistent with a transfer of outdoor traffic emissions to the indoor classrooms. This observation was supported by the hopane and elemental carbon concentrations in PM2.5, markers of motorized vehicles, that were correlated with PAHs. The concentrations of food-related markers, such as glucoses, sucrose, malic, azelaic and fatty acids, were correlated and were higher in the indoor atmospheres. These compounds were also correlated with plastic additives, such as phthalic acid and diisobutyl, dibutyl and dicyclohexyl phthalates. Clothing constituents, e.g., adipic acid, and fragrances, galaxolide and methyl dihydrojasmonate were also correlated with these indoor air compounds. All these organic tracers were correlated with the organic carbon of PM2.5, which was present in higher concentrations in the indoor than in the outdoor atmospheres.
A Study of Using Natural Sorbent to Reduce Iron Cations from Aqueous Solutions
Authors: Pandová Iveta , Rimár Miroslav , Panda Anton , Valíček Jan , Kušnerová Milena , Harničárová Marta
Published: May 2020

Iron is an essential trace element, but at high doses, this element may pose a health risk. Wastewater from iron ore mining, steel production, and metal processing, among other heavy metals, also contains high concentrations of iron (Fe3+). The use of sorption on natural materials is a potential alternative to conventional methods for removing iron ions, also because of low cost. The methods presented in this article are based on the study of kinetic properties and the acquisition of adsorption isotherms, which are one of the most important characteristics of adsorption mechanisms. The course of sorption is analyzed according to the Freundlich sorption isotherm model. Isotherm parameters are evaluated using experimental results of ferric cation sorption. The results presented relate to the investigation of natural zeolite-clinoptilolite as a ferric cation sorbent, providing a measurement of the sorption kinetics as well as the observed sorption parameters of iron cations from aqueous media. The optimal time for equilibrium in the adsorption system is determined from the kinetic dependencies. The dependence of the achieved equilibrium concentration on the initial concentration of the solution was also expressed, both graphically and analytically. The new prediction model was compared with the traditional Freundlich model. Finally, adsorption isotherms tested under laboratory conditions for a practical application can be recommended for the preliminary examination of the possible technological use of natural zeolite in the wastewater treatment process.
Effects of the Use of Air Purifier on Indoor Environment and Respiratory System among Healthy Adults
Authors: Yoda Yoshiko , Tamura Kenji , Adachi Sho , Otani Naruhito , Nakayama Shoji F. , Shima Masayuki
Published: May 2020

Air purifiers have become popular among ordinary families. However, it remains controversial whether indoor air purification improves the respiratory health of healthy adults. A randomized crossover intervention study was conducted with 32 healthy individuals. The subjects were categorized into two groups. One group continuously used true air purifiers, and the other followed with sham air purifiers for 4 weeks. Following this first intervention, all the subjects underwent a 4-week washout period and continued with the second 4-week intervention with the alternate air purifiers. We collected fine particulate matter (PM) ≤ 2.5 µm in aerodynamic diameter (PM2.5), coarse particulate matter between 2.5 and 10 µm in aerodynamic diameter (PM10–2.5) and ozone (O3). The subjects’ pulmonary function and fractional exhaled nitric oxide (FeNO) were measured during the study period. The indoor PM2.5 concentrations decreased by 11% with the true air purifiers compared to those with sham air purifiers. However, this decrease was not significant (p = 0.08). The air purification did not significantly improve the pulmonary function of the study subjects. In contrast, an increase in the indoor PM10–2.5 and O3 concentration led to a significant decrease in the forced expiratory volume in one second (FEV1.0)/forced vital capacity (FVC) and maximal mid-expiratory flow (MMEF), respectively. In conclusion, air purification slightly improved the indoor PM2.5 concentrations in ordinary homes but had no demonstrable impact on improving health.
Exposure to Environmental Tobacco Smoke (ETS) among Employees of Hospitality Venues in the Light of Changes in Anti-Tobacco Legislation in Poland
Authors: Krakowiak Emilia , Sygit Katarzyna , Sygit Marian , Cipora Elżbieta , Krakowiak Jan
Published: May 2020

Introduction: Numerous studies conducted in Europe and worldwide have indicated that employees of hospitality venues are the most exposed professional group to environmental tobacco smoke (ETS) in the workplace. The purpose of this study was to assess the exposure of employees of hospitality venues to ETS in the light of changes in anti-tobacco legislation in Poland. Materials and methods: The study consisted of two stages. The first stage was conducted in 2010, while the second in 2015. The study was conducted among employees of 300 randomly selected hospitality venues in the city of Łódź (Poland). In total, 2607 questionnaires were analysed. The study used two survey questionnaires created and recommended by the Institute for Global Tobacco Control to study exposure to ETS. Statistical analysis was made with Statistica 13.1 PL (StatSoft, Poland). Results: In the group of all nonsmoking employees, individuals exposed to ETS at work in 2010 accounted for 72.6%; while in 2015 it was 51.8%. Factors affecting exposure to ETS in the workplace included, among others: age, marital status, education, position held, presence of a smoking room on the premises, and noncompliance with the provisions of the anti-tobacco laws. Conclusions: The prevalence of tobacco smoking among employees of hospitality venues decreased in 2010–2015, however, it remained high. More than half of nonsmoking employees were exposed to ETS at work.
The Impact of Cybervictimization on Psychological Adjustment in Adolescence: Analyzing the Role of Emotional Intelligence
Authors: Estévez Jesús F. , Cañas Elizabeth , Estévez Estefanía
Published: May 2020

Cybervictimization has been associated with serious emotional adjustment problems such as low self-concept and depressive symptomatology. In addition, these problems can negatively affect the well-being of the victims, manifesting in their levels of satisfaction with life. However, it should be noted that not all cybervictims develop these consequences with the same intensity. These differences seem to be related to the development of emotional intelligence (EI), as it can positively influence adolescents’ emotional adjustment and well-being even when problems arise. The objective of this work was to analyze the role of EI on cybervictimization and adolescents’ emotional adjustment, especially in self-concept, depression, and life satisfaction. The participants in the study were 1318 adolescents of both sexes and aged between 11 and 18 years (M = 13.8, SD = 1.32), from four secondary compulsory education centers in Spain. EI influences the relationship between self-concept and life satisfaction, and between depression and life satisfaction. In addition, the relationships of cybervictimization with self-concept and depression are influenced when introducing EI and its dimensions (emotional attention, clarity, regulation). These data support the idea that EI may affect the relationship between cybervictimization and adolescents’ emotional adjustment.
Opinions and Knowledge of Parents Regarding Preventive Vaccinations of Children and Causes of Reluctance toward Preventive Vaccinations
Authors: Lewandowska Anna , Lewandowski Tomasz , Rudzki Grzegorz , Rudzki Sławomir , Laskowska Barbara
Published: May 2020

Background: Despite the stability of global vaccination coverage, over 19 million children worldwide do not currently receive basic vaccines. Over the past several years, there has been a dramatic drop in the number of vaccinated children worldwide. The implementation of the vaccination program and the scope of protection depend on the parents or legal guardians, who decide whether to vaccinate their child or not. Studies were conducted to assess parents’ knowledge, attitudes, and beliefs about vaccines, as well as the role of healthcare providers in parents’ decisions. Methods: A population survey was conducted in 2018–2019. Parents or legal guardians of the children were invited to participate in the study during their visits to the clinic for healthy or sick children. The method used in the research was a diagnostic survey. Results: According to the conducted research, men and women constituted 45% and 55% of participants, respectively. The average age of men was 44, while, for women, it was 41. Internal research showed that as much as 71% of parents declared the need for vaccination, although 41% of parents vaccinated their children according to the vaccination calendar. The most frequently mentioned concerns included the possibility of adverse vaccination reactions (22%), the occurrence of autism (7%), and child death (6%). General practitioners had, by far, the greatest impact on the use of protective vaccination in children (73% women and 80% men), although there were cases of discouraging the performance of compulsory vaccinations (41%), and mentioning a doctor (38%) or nurse (3%). Conclusions: Modifiable determinants of the negative attitude toward vaccinations are caused mainly by the lack of knowledge. These obstacles in vaccinations can be overcome by improving health education in terms of the vaccination program.
Do Age, Gender and Poor Diet Influence the Higher Prevalence of Nomophobia among Young People?
Authors: Moreno-Guerrero Antonio-José , Aznar-Díaz Inmaculada , Cáceres-Reche Pilar , Rodríguez-García Antonio-Manuel
Published: May 2020

The use of Information and Communication Technologies (ICT) is generating the emergence of new pathologies such as nomophobia. The aim of this research was to analyze the prevalence of nomophobia among young people, as well as to check whether the level of nomophobia is higher in males or females and in those students who claim to have less healthy nutrition due to the use of their mobile phones. The research method was based on a correlational and predictive design with a quantitative methodology. The measurement tool used is the Nomophobia Questionnaire (NMP-Q). The participating sample was 1743 students between 12 and 20 years old from different educational stages of the Autonomous City of Ceuta (Spain). The results show that highest rates of nomophobia were found in relation to the inability to communicate and contact others immediately. About gender, women have higher rates of nomophobia than men. In relation to age, no significant differences were found; thus, the problem may affect all ages equally. Finally, students who think that their smartphone use is detrimental to their good nutrition show higher levels on the scale provided.