bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of High Performance Self-Consolidating Concrete through an Experimental and Analytical Multi-Parameter Approach
Authors: Ahmed Ghafur H. , Ahmed Hawreen , Ali Babar , Alyousef Rayed
Published: February 2021

High-performance self-consolidating concrete is one of the most promising developments in the construction industry. Nowadays, concrete designers and ready-mix companies are seeking optimum concrete in terms of environmental impact, cost, mechanical performance, as well as fresh-state properties. This can be achieved by considering the mentioned parameters simultaneously; typically, by integrating conventional concrete systems with different types of high-performance waste mineral admixtures (i.e., micro-silica and fly ash) and ultra-high range plasticizers. In this study, fresh-state properties (slump, flow, restricted flow), hardened-state properties (density, water absorption by immersion, compressive strength, splitting tensile strength, flexural strength, stress-strain relationship, modulus of elasticity, oven heating test, fire-resistance, and freeze-thaw cycles), and cost of high-performance self-consolidating concrete (HPSCC) prepared with waste mineral admixtures, were examined and compared with three different reference mixes, including normal strength-vibrated concrete (NSVC), high-strength self-compacted concrete (HSSCC), and high-performance highly-viscous concrete (HPVC). Then, a multi parameter analytical approach was considered to identify the optimum concrete mix in terms of cost, workability, strength, and durability.
Lactoferrin/Calcium Phosphate-Modified Porous Ti by Biomimetic Mineralization: Effective Infection Prevention and Excellent Osteoinduction
Authors: Chen Song , He Yuanli , Zhong Linna , Xie Wenjia , Xue Yiyuan , Wang Jian
Published: February 2021

The surface modification of titanium (Ti) can enhance the osseointegration and antibacterial properties of implants. In this study, we modified porous Ti discs with calcium phosphate (CaP) and different concentrations of Lactoferrin (LF) by biomimetic mineralization and examined their antibacterial effects and osteogenic bioactivity. Firstly, scanning electron microscopy (SEM), the fluorescent tracing method, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and the releasing kinetics of LF were utilized to characterize the modified Ti surface. Then, the antibacterial properties against S. sanguis and S. aureus were investigated. Finally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell differentiation, extracellular matrix mineralization, and cytotoxicity. The results showed that the porous Ti discs were successfully modified with CaP and LF, and that the LF-M group (200 μg/mL LF in simulated body fluid) could mildly release LF under control. Further, the LF-M group could effectively inhibit the adhesion and proliferation of S. sanguis and S. aureus and enhance the osteogenic differentiation in vitro with a good biocompatibility. Consequently, LF-M-modified Ti may have potential applications in the field of dental implants to promote osseointegration and prevent the occurrence of peri-implantitis.
A Proposal of Quantum-Inspired Machine Learning for Medical Purposes: An Application Case
Authors: Pomarico Domenico , Fanizzi Annarita , Amoroso Nicola , Bellotti Roberto , Biafora Albino , Bove Samantha , Didonna Vittorio , Forgia Daniele La , Pastena Maria Irene , Tamborra Pasquale , Zito Alfredo , Lorusso Vito , Massafra Raffaella
Published: February 2021

Learning tasks are implemented via mappings of the sampled data set, including both the classical and the quantum framework. Biomedical data characterizing complex diseases such as cancer typically require an algorithmic support for clinical decisions, especially for early stage tumors that typify breast cancer patients, which are still controllable in a therapeutic and surgical way. Our case study consists of the prediction during the pre-operative stage of lymph node metastasis in breast cancer patients resulting in a negative diagnosis after clinical and radiological exams. The classifier adopted to establish a baseline is characterized by the result invariance for the order permutation of the input features, and it exploits stratifications in the training procedure. The quantum one mimics support vector machine mapping in a high-dimensional feature space, yielded by encoding into qubits, while being characterized by complexity. Feature selection is exploited to study the performances associated with a low number of features, thus implemented in a feasible time. Wide variations in sensitivity and specificity are observed in the selected optimal classifiers during cross-validations for both classification system types, with an easier detection of negative or positive cases depending on the choice between the two training schemes. Clinical practice is still far from being reached, even if the flexible structure of quantum-inspired classifier circuits guarantees further developments to rule interactions among features: this preliminary study is solely intended to provide an overview of the particular tree tensor network scheme in a simplified version adopting just product states, as well as to introduce typical machine learning procedures consisting of feature selection and classifier performance evaluation.
Dental Care in Times of the COVID-19 Pandemic: A Review
Authors: Shamsoddin Erfan , DeTora Lisa M. , Tovani-Palone Marcos Roberto , Bierer Barbara E.
Published: February 2021

Given the dynamic relationship between oral and general health, dental care must not be neglected even during a public health emergency. Nevertheless, the fear of contracting the infection appears to have caused instances of dental treatment avoidance. In these times of uncertainty, regulatory and public health organizations have made numerous and sometimes controversial recommendations to practitioners and to the public about how to secure their oral health care needs. Dentists, as advocates of oral health, should actively maintain their practices while considering local epidemiological reports and recommendations regarding prevention of SARS-CoV-2 infection. Providing appropriate safety measures, accurate triage and prioritization of patients, notice to susceptible communities, remote health care delivery when appropriate, and epidemiological reports of COVID-19 (whenever possible) are all critical considerations for dental practitioners.
Failure Mechanism of Gun Barrel Caused by Peeling of Cr Layer and Softening of Bore Matrix
Authors: Wang Zi-meng , Hu Chun-dong , Wang Yang-xin , Lu Heng-chang , Li Jun-song , Dong Han
Published: February 2021

Research on failure mechanism is essential for the prolonging of gun barrel lifetime. To explore the gun barrel failure mechanism, the damage characteristics of a machine gun barrel were characterized. The results show that the failure of the gun barrel is correlated with the peeling of the Cr layer on the bore surface and the softening of the bore matrix. The damage of the Cr layer varies along the axial direction. From the gun tail to the muzzle, the damage mode of the Cr layer changes from peeling to wearing. The damage rate in both the tail and the muzzle is higher than that in the middle of the barrel. The matrix close to the bore surface is softened due to the high temperatures caused by stress–relief–annealing and shooting. The gun tail suffers from higher temperatures, thus being softened more seriously than the other parts. The softened matrix results in an increase in the tendency of plastic deformation of the bore surface and an increment in the bore diameter, which leads to a decrease in the firing accuracy.
Effect of Mn Addition on Hot-Working Behavior and Microstructure of Hot-Rolled Medium-Mn Steels
Authors: Skowronek Adam , Woźniak Dariusz , Grajcar Adam
Published: February 2021

Hot plastic working behavior and microstructure evolution were investigated during a production process of four medium-Mn steels, which differed in Mn (3 and 5%) and Nb contents. The production process started with casting, followed by hot forging, rough hot-rolling and concluded with final thermomechanical processing, which was performed to obtain multiphase bainite-based alloys with some fractions of retained austenite. The rough rolling was composed of four passes with total true strain of 0.99 and finishing rolling temperature of 850 °C, whereas thermomechanical processing contained five passes and total true strain of 0.95 at a finishing rolling temperature of 750 °C. During the process, the force parameters were recorded, which showed that the rolling forces for steels containing 3% Mn are higher compared to the 5% Mn alloys. There was no significant influence of Nb on the rolling parameters. The produced as-cast microstructures were composed of dendritic bainitic-martensitic phases. A positive effect of Nb micro-addition on a refinement of the as-cast structure was noticed. The thermomechanical processed steels showed fine multiphase microstructures with some fractions of retained austenite, the fraction of which depended on the Mn content in steel. The steels containing 3% Mn generated higher forces both during rough and thermomechanical rolling, which is related to slower recrystallization softening in these alloys compared to the steels containing 5% Mn.
Hydrogen Embrittlement of Medium Mn Steels
Authors: Cho Lawrence , Kong Yuran , Speer John G. , Findley Kip O.
Published: February 2021

Recent research efforts to develop advanced–/ultrahigh–strength medium-Mn steels have led to the development of a variety of alloying concepts, thermo-mechanical processing routes, and microstructural variants for these steel grades. However, certain grades of advanced–/ultrahigh–strength steels (A/UHSS) are known to be highly susceptible to hydrogen embrittlement, due to their high strength levels. Hydrogen embrittlement characteristics of medium–Mn steels are less understood compared to other classes of A/UHSS, such as high Mn twinning–induced plasticity steel, because of the relatively short history of the development of this steel class and the complex nature of multiphase, fine-grained microstructures that are present in medium–Mn steels. The motivation of this paper is to review the current understanding of the hydrogen embrittlement characteristics of medium or intermediate Mn (4 to 15 wt pct) multiphase steels and to address various alloying and processing strategies that are available to enhance the hydrogen-resistance of these steel grades.
In Vitro Analysis of the Antioxidant and Antiviral Activity of Embelin against Herpes Simplex Virus-1
Authors: Elias Tony , Lee Lee H. , Rossi Miriam , Caruso Francesco , Adams Sandra D.
Published: February 2021

Herpes simplex virus-1 (HSV-1) causes a wide range of infections from mild to life-threatening in the human population. There are effective treatments for HSV-1 infections that are limited due HSV-1 latency and development of resistance to current therapeutics. The goal of this study was to investigate the antioxidant and antiviral effects of embelin on HSV-1 in cultured Vero cells. Oxidative stress was verified by an extensive production of a reactive oxygen species (ROS) H2O2. Vero cells were infected with a recombinant strain of HSV-1 and antiviral assays, time course attachment, penetration, and post penetration assays, confocal microscopy, qPCR, and antioxidant assays were conducted. Our results lead to the conclusion that embelin is noncytotoxic at concentrations tested ranging from 20 to 70 µM. Treatment of HSV-1 virions with embelin resulted in 98.7–100% inhibition and affected the early stage of HSV-1 infection of Vero cells, by inhibiting the attachment and penetration of HSV-1 virions to host cells. Treatment of virions with concentrations of embelin ranging from 35 to 60 µM significantly reduced the production of H2O2. In conclusion, embelin reduces oxidative damage caused by HSV-1 infection and is an effective antiviral to reduce the infection of HSV-1 in cultured Vero cells. Further studies are needed to explore the possibility of embelin as a medicinal agent.
RORα Regulates Odontoblastic Differentiation and Mediates the Pro-Odontogenic Effect of Melatonin on Dental Papilla Cells
Authors: Kang Jun , Chen Haoling , Zhang Fuping , Yan Tong , Fan Wenguo , Jiang Liulin , He Hongwen , Huang Fang
Published: February 2021

Dental papilla cells (DPCs), precursors of odontoblasts, are considered promising seed cells for tissue engineering. Emerging evidence suggests that melatonin promotes odontoblastic differentiation of DPCs and affects tooth development, although the precise mechanisms remain unknown. Retinoid acid receptor-related orphan receptor α (RORα) is a nuclear receptor for melatonin that plays a critical role in cell differentiation and embryonic development. This study aimed to explore the role of RORα in odontoblastic differentiation and determine whether melatonin exerts its pro-odontogenic effect via RORα. Herein, we observed that RORα was expressed in DPCs and was significantly increased during odontoblastic differentiation in vitro and in vivo. The overexpression of RORα upregulated the expression of odontogenic markers, alkaline phosphatase (ALP) activity and mineralized nodules formation (p < 0.05). In contrast, odontoblastic differentiation of DPCs was suppressed by RORα knockdown. Moreover, we found that melatonin elevated the expression of odontogenic markers, which was accompanied by the upregulation of RORα (p < 0.001). Utilising small interfering RNA, we further demonstrated that RORα inhibition attenuated melatonin-induced odontogenic gene expression, ALP activity and matrix mineralisation (p < 0.01). Collectively, these results provide the first evidence that RORα can promote odontoblastic differentiation of DPCs and mediate the pro-odontogenic effect of melatonin.
Efficient Regioselective Synthesis of Novel Water-Soluble 2H,3H-[1,4]thiazino[2,3,4-ij]quinolin-4-ium Derivatives by Annulation Reactions of 8-quinolinesulfenyl Halides
Authors: Potapov Vladimir A. , Ishigeev Roman S. , Amosova Svetlana V.
Published: February 2021

Regioselective synthesis of novel 2H,3H-[1,4]thiazino[2,3,4-ij]quinolin-4-ium derivatives has been developed by annulation reactions of 8-quinolinesulfenyl halides with vinyl chalcogenides (vinyl ethers, divinyl sulfide, divinyl selenide and phenyl vinyl sulfide) and tetravinyl silane. The novel reagent 8-quinolinesulfenyl bromide was used in the annulation reactions. The influence of the substrate structure and the nature of heteroatoms on the direction of the reactions and on product yields has been studied. The opposite regiochemistry was observed in the reactions with vinyl chalcogenides and tetravinyl silane. The obtained condensed heterocycles are novel water-soluble functionalized compounds with promising biological activity.
Solution-Processed OLEDs Based on Thermally Activated Delayed Fluorescence Copper(I) Complexes with Intraligand Charge-Transfer Excited State
Authors: Teng Teng , Xiong Jinfan , Cheng Gang , Zhou Changjiang , Lv Xialei , Li Kai
Published: February 2021

A new series of tetrahedral heteroleptic copper(I) complexes exhibiting efficient thermally-activated delayed fluorescence (TADF) in green to orange electromagnetic spectral regions has been developed by using D-A type N^N ligand and P^P ligands. Their structures, electrochemical, photophysical, and electroluminescence properties have been characterized. The complexes exhibit high photoluminescence quantum yields (PLQYs) of up to 0.71 at room temperature in doped film and the lifetimes are in a wide range of 4.3–24.1 μs. Density functional theory (DFT) calculations on the complexes reveal the lowest-lying intraligand charge-transfer excited states that are localized on the N^N ligands. Solution-processed organic light emitting diodes (OLEDs) based on one of the new emitters show a maximum external quantum efficiency (EQE) of 7.96%.
Nonlinear Optical Response of Gold Nanobipyramids for a Doubly Q-Switched Ho-Doped Laser at a Wavelength of 2.1 µm
Authors: Zhang Cheng , Wang Dongzhou , Huang Shengjun , Yang Jimin , Liu Jie , Fang Jing
Published: February 2021

Gold nanobipyramids (Au-NBPs) were successfully fabricated using the seed-mediated growth method. The saturable absorption performance of the Au-NBPs at a 2-μm band wavelength was characterized. Using excellent-quality, mature Ho:YLF crystals, a doubly Q-switched (DQS) laser joining an acousto-optic modulator (AOM) with an Au-NBP saturable absorber (SA) was achieved. When the modulation rate of the AOM was 1 kHz, the shortest pulse width (54 ns) was attained, corresponding to the highest peak power (3.87 kW). This was compared with a singly Q-switched laser joining an AOM with an Au-NBP SA, whereby the maximum pulse width compression ratio was 15.2 and the highest peak power enhancement factor was 541.3. Our study has shown that Au-NBPs are a potential saturable absorption nanomaterial, and the DQS laser has the benefit of compressing the pulse width and increasing the peak power at a wavelength of 2.1 μm.
Preparation and Characterization of Electrospun Fluoro-Containing Poly(imide-benzoxazole) Nano-Fibrous Membranes with Low Dielectric Constants and High Thermal Stability
Authors: Huangfu Meng-Ge , Shen Deng-Xiong , Zhi Xin-Xin , Zhang Yan , Jia Yan-Jiang , An Yuan-Cheng , Wei Xin-Ying , Liu Jin-Gang
Published: February 2021

The rapid development of advanced high-frequency mobile communication techniques has advanced urgent requirements for polymer materials with high-temperature resistance and good dielectric properties, including low dielectric constants (low-Dk) and low dielectric dissipation factors (low-Df). The relatively poor dielectric properties of common polymer candidates, such as standard polyimides (PIs) greatly limited their application in high-frequency areas. In the current work, benzoxazole units were successfully incorporated into the molecular structures of the fluoro-containing PIs to afford the poly(imide-benzoxazole) (PIBO) nano-fibrous membranes (NFMs) via electrospinning fabrication. First, the PI NFMs were prepared by the electrospinning procedure from organo-soluble PI resins derived from 2,2′-bis(3,4-dicarboxy-phenyl)hexafluoropropane dianhydride (6FDA) and aromatic diamines containing ortho-hydroxy-substituted benzamide units, including 2,2-bis[3-(4-aminobenzamide)-4-hydroxylphenyl]hexafluoropropane (p6FAHP) and 2,2-bis[3-(3-aminobenzamide)-4-hydroxyphenyl]hexafluoropropane (m6FAHP). Then, the PI NFMs were thermally dehydrated at 350 °C in nitrogen to afford the PIBO NFMs. The average fiber diameters (dav) for the PIBO NFMs were 1225 nm for PIBO-1 derived from PI-1 (6FDA-p6FAHP) precursor and 816 nm for PIBO-2 derived from PI-2 (6FDA-m6FAHP). The derived PIBO NFMs showed good thermal stability with the glass transition temperatures (Tgs) over 310 °C and the 5% weight loss temperatures (T5%) higher than 500 °C in nitrogen. The PIBO NFMs showed low dielectric features with the Dk value of 1.64 for PIBO-1 and 1.82 for PIBO-2 at the frequency of 1 MHz, respectively. The Df values were in the range of 0.010~0.018 for the PIBO NFMs.
Cardiorespiratory Fitness Predicted by Fibrinogen and Leptin Concentrations in Children with Obesity and Risk for Diabetes: A Cross-Sectional Study and a ROC Curve Analysis
Authors: Tsiroukidou Kyriaki , Hatziagorou Elpis , Grammatikopoulou Maria G. , Vamvakis Anastasios , Kontouli Kalliopi , Tzimos Christos , Tsanakas John , Spiliotis Bessie E.
Published: February 2021

Obesity is defined as abnormal or excessive fat accumulation that presents a risk to health. The ability to exercise is affected by adiposity, and this mechanism involves low-grade chronic inflammation and homeostatic stress produced mainly in adipocytes, which can result in abnormal adipokine secretion. To date, the gold standard for cardiorespiratory fitness assessment is considered to be the maximum oxygen uptake (VO2max). The aim of the present study was to assess the prognostic value of hematological parameters of childhood obesity, as potential predictors of cardiorespiratory fitness (VO2max), using a sample of children and adolescents with obesity and risk for diabetes. A total of 84 clinically healthy children and adolescents were recruited, of which 21 were considered lean, 22 overweight and 41 obese, with a mean age of 12.0 ± 1.9, 11.4 ± 2.0, and 11.2 ± 2.1 years old, in each weight status category, respectively. Age and sex did not differ between groups. Hematologic testing was performed after 12 h of fasting including glucose, serum lipids, insulin, hc-CRP, adiponectin, leptin and fibrinogen levels. Cardiorespiratory capacity for exercise was assessed to determine VO2max, using a cycle ergometer. The VO2max was negatively correlated with progressive strength to the BMIz (−0.656, p ≤ 0.001), hs-CRP (r = −0.341, p ≤ 0.002), glucose (r = −0.404, p ≤ 0.001) and insulin levels (r = −0.348, p ≤ 0.001), the homeostasis model assessment of insulin resistance (HOMA-IR) (r = −0.345, p ≤ 0.002), as well as to the leptin (r = −0.639, p ≤ 0.001) and fibrinogen concentrations (r = −0.520, p ≤ 0.001). The multivariate analysis revealed that only leptin and fibrinogen concentrations could predict the VO2max adjusted for the BMIz of participants. The receiver operating characteristic (ROC) curve for the diagnostic accuracy of leptin, hs-CRP and fibrinogen concentrations for the prediction of VO2max revealed a good diagnostic ability for all parameters, with leptin being the most promising one (area under the curve (AUC): 99%). The results verify that in children with obesity, VO2max may be predicted from hematological parameters (leptin and fibrinogen), possibly bypassing more invasive methods.
ACE Inhibitory and Antihypertensive Activities of Wine Lees and Relationship among Bioactivity and Phenolic Profile
Authors: López-Fernández-Sobrino Raúl , Soliz-Rueda Jorge R. , Margalef Maria , Arola-Arnal Anna , Suárez Manuel , Bravo Francisca I. , Muguerza Begoña
Published: February 2021

Wine lees (WL) are by-products generated in the winemaking process. The aim of this study was to investigate the angiotensin-converting enzyme inhibitory (ACEi) activity, and the blood pressure (BP) lowering effect of WL from individual grape varieties. The relationship among their activities and phenolic profiles was also studied. Three WL, from Cabernet, Mazuela, and Garnacha grape varieties, were firstly selected based on their ACEi properties. Their phenolic profiles were fully characterized by UHPLC-ESI-Q-TOF-MS. Then, their potential antihypertensive effects were evaluated in spontaneously hypertensive rats (SHR). BP was recorded before and after their oral administrations (2, 4, 6, 8, 24, and 48 h) at a dose of 5 mL/kg bw. Cabernet WL (CWL) exhibited a potent antihypertensive activity, similar to that obtained with the drug Captopril. This BP-lowering effect was related to the high amount of anthocyanins and flavanols present in these lees. In addition, a potential hypotensive effect of CWL was discarded in normotensive Wistar–Kyoto rats. Finally, the ACEi and antihypertensive activities of CWL coming from a different harvest were confirmed. Our results suggest the potential of CWL for controlling arterial BP, opening the door to commercial use within the wine industry.
Nosocomial Infections: Do Not Forget the Parasites!
Authors: Fürnkranz Ursula , Walochnik Julia
Published: February 2021

Nosocomial infections (NIs) pose an increasing threat to public health. The majority of NIs are bacterial, fungal, and viral infections; however, parasites also play a considerable role in NIs, particularly in our increasingly complex healthcare environment with a growing proportion of immunocompromised patients. Moreover, parasitic infections acquired via blood transfusion or organ transplantation are more likely to have severe or fatal disease outcomes compared with the normal route of infection. Many of these infections are preventable and most are treatable, but as the awareness for parasitic NIs is low, diagnosis and treatment are often delayed, resulting not only in higher health care costs but, importantly, also in prolonged courses of disease for the patients. For this article, we searched online databases and printed literature to give an overview of the causative agents of parasitic NIs, including the possible routes of infection and the diseases caused. Our review covers a broad spectrum of cases, ranging from widely known parasitic NIs, like blood transfusion malaria or water-borne cryptosporidiosis, to less well-known NIs, such as the transmission of Strongyloides stercoralis by solid organ transplantation or nosocomial myiasis. In addition, emerging NIs, such as babesiosis by blood transfusion or person-to-person transmitted scabies, are described.
Staphylococcus aureus Internalization in Osteoblast Cells: Mechanisms, Interactions and Biochemical Processes. What Did We Learn from Experimental Models?
Authors: Stracquadanio Stefano , Musso Nicolò , Costantino Angelita , Lazzaro Lorenzo Mattia , Stefani Stefania , Bongiorno Dafne
Published: February 2021

Bacterial internalization is a strategy that non-intracellular microorganisms use to escape the host immune system and survive inside the human body. Among bacterial species, Staphylococcus aureus showed the ability to interact with and infect osteoblasts, causing osteomyelitis as well as bone and joint infection, while also becoming increasingly resistant to antibiotic therapy and a reservoir of bacteria that can make the infection difficult to cure. Despite being a serious issue in orthopedic surgery, little is known about the mechanisms that allow bacteria to enter and survive inside the osteoblasts, due to the lack of consistent experimental models. In this review, we describe the current knowledge about S. aureus internalization mechanisms and various aspects of the interaction between bacteria and osteoblasts (e.g., best experimental conditions, bacteria-induced damages and immune system response), focusing on studies performed using the MG-63 osteoblastic cell line, the best traditional (2D) model for the study of this phenomenon to date. At the same time, as it has been widely demonstrated that 2D culture systems are not completely indicative of the dynamic environment in vivo, and more recent 3D models—representative of bone infection—have also been investigated.
Isolation and Characterization of Kingella bonacorsii sp. nov., A Novel Kingella Species Detected in a Stable Periodontitis Subject
Authors: Antezack Angéline , Boxberger Manon , Rolland Clara , Monnet-Corti Virginie , La Scola Bernard
Published: February 2021

Members of the genus Kingella are mostly commensals of the oral cavity, but some of them are involved in invasive infections, especially in young children. This study provides new knowledge on the diversity of this genus by describing a novel species of Kingella isolated from a dental plaque sample from a 51-year-old man with a history of periodontitis. Morphological and chemotaxonomic characteristic were investigated using different growth conditions, pH and temperature. Cellular fatty acid methyl ester (FAME) analysis was performed by gas chromatography/mass spectrometry (GC/MS). Phylogenetic analysis based on 16S rRNA, orthologous average nucleotide identity (OrthoANI) and digital DNA–DNA hybridization (dDDH) relatedness were also performed. Strain Marseille-Q4569T was found to be a facultative aerobic, nonmotile and non-spore-forming rod-shaped bacterium that grows at 28–41.5 °C (optimum 37 °C), pH 5.5–8.5 (optimum pH 7.5) and 5–15 g/L of NaCl. The major fatty acids were Hexadecanoic acid (32.7%), 11-Octadecenoic acid (26.1 %) and 9-Hexadecenoic acid (21.3 %). Despite high 16S rRNA gene sequence similarity (98.72%) between strain Marseille-Q4569T and Kingella oralis strain UB-38T, the degree of OrthoANI was at the limit of the cutoff (95.83%), and the degree of dDDH was lower (63.6%) than thresholds used to delineate prokaryotic species. Therefore, it is proposed that strain Marseille-Q4569T represents a novel species of the genus Kingella, for which the name Kingella bonacorsii sp. nov. is proposed (=CSUR Q4569).
Towards the Development of Long Circulating Phosphatidylserine (PS)- and Phosphatidylglycerol (PG)-Enriched Anti-Inflammatory Liposomes: Is PEGylation Effective?
Authors: Klein Miriam E. , Rieckmann Max , Sedding Daniel , Hause Gerd , Meister Annette , Mäder Karsten , Lucas Henrike
Published: February 2021

The anionic phospholipids (PLs) phosphatidylserine (PS) and phosphatidylglycerol (PG) are endogenous phospholipids with anti-inflammatory and immunomodulatory activity. A potential clinical use requires well-defined systems and for several applications, a long circulation time is desirable. Therefore, we aimed the development of long circulating liposomes with intrinsic anti-inflammatory activity. Hence, PS- and PG-enriched liposomes were produced, whilst phosphatidylcholine (PC) liposomes served as control. Liposomes were either formulated as conventional or PEGylated formulations. They had diameters below 150 nm, narrow size distributions and composition-dependent surface charges. Pharmacokinetics were assessed non-invasively via in vivo fluorescence imaging (FI) and ex vivo in excised organs over 2 days. PC liposomes, conventionally formulated, were rapidly cleared from the circulation, while PEGylation resulted in prolongation of liposome circulation robustly distributing among most organs. In contrast, PS and PG liposomes, both as conventional or PEGylated formulations, were rapidly cleared. Non-PEGylated PS and PG liposomes did accumulate almost exclusively in the liver. In contrast, PEGylated PS and PG liposomes were observed mainly in liver and spleen. In summary, PEGylation of PS and PG liposomes was not effective to prolong the circulation time but caused a higher uptake in the spleen.
On Absorption Modeling and Food Effect Prediction of Rivaroxaban, a BCS II Drug Orally Administered as an Immediate-Release Tablet
Authors: Kushwah Varun , Arora Sumit , Tamás Katona Miklós , Modhave Dattatray , Fröhlich Eleonore , Paudel Amrit
Published: February 2021

The present work evaluates the food effect on the absorption of rivaroxaban (Riva), a BCS II drug, from the orally administered commercial immediate-release tablet (Xarelto IR) using physiologically based pharmacokinetic (PBPK) and conventional in vitro–in vivo correlation (IVIVC) models. The bioavailability of Riva upon oral administration of Xarelto IR tablet is reported to exhibit a positive food effect. The PBPK model for Riva was developed and verified using the previously reported in vivo data for oral solution (5 and 10 mg) and Xarelto IR tablet (5 and 10 mg dose strength). Once the PBPK model was established, the in vivo performance of the tablet formulation with the higher dose strength (Xarelto IR tablet 20 mg in fasted and fed state) was predicted using the experimentally obtained data of in vitro permeability, biorelevant solubility and in vitro dynamic dissolution data using United States Pharmacopeia (USP) IV flow-through cell apparatus. In addition, the mathematical IVIVC model was developed using the in vitro dissolution and in vivo profile of 20 mg strength Xarelto IR tablet in fasted condition. Using the developed IVIVC model, the pharmacokinetic (PK) profile of the Xarelto IR tablet in fed condition was predicted and compared with the PK parameters obtained via the PBPK model. A virtual in vivo PK study was designed using a single-dose, 3-treatment cross-over trial in 50 subjects to predict the PK profile of the Xarelto® IR tablet in the fed state. Overall, the results obtained from the IVIVC model were found to be comparable with those from the PBPK model. The outcome from both models pointed to the positive food effect on the in vivo profile of the Riva. The developed models thus can be effectively extended to establish bioequivalence for the marketed and novel complex formulations of Riva such as amorphous solid dispersions.
Joint Estimation of Symbol Rate and Chromatic Dispersion Using Delayed Multiplier for Optical Performance Monitoring
Authors: Ba Junhao , Huang Zhiping , Shen Fangqi , Wei Junyu
Published: February 2021

Symbol rate and chromatic dispersion (CD) are very important for optical performance monitoring. The CD, however, hinders the symbol rate detection. In this paper, we proposed a joint estimation of symbol rate and chromatic dispersion. We show that, when the signal conjugates and multiplies with the delayed replica, the spectral line can be restored. The proposed method provides a fast and simple solution for joint estimation as traditional tentative CD scanning is time consuming. The simulation shows that the root-mean-squared error (RMSE) for CD was 39.5 ps/nm and the symbol rate was 2.4 MHz. For the squared-root-raised-cosine (SRRC) pulse shape with a roll-off factor of 0.1, the experimental results show that 25,000 input samples were needed for an error-free estimation. The RMSE is 105.6 ps/nm and 63.5 kHz for CD and symbol rate, respectively.
Identification and Detection of Botryosphaeria dothidea from Kiwifruit (Actinidia chinensis) in China
Authors: Wang Li , Hou Hui , Zhou Zengqiang , Tu Hongtao , Yuan Hongbo
Published: February 2021

Kiwifruit is very popular among consumers due to its high nutritional value. The increasing expansion in kiwifruit cultivation has led to the spread of rot diseases. To identify the pathogens causing kiwifruit ripe rots in China, 24 isolates were isolated from the diseased fruit and wart in trees. Botryosphaeria dothidea was recognized as the pathogen causing kiwifruit ripe rot and wart in the tree through internal transcribed spacer (ITS) sequencing, pathogenicity testing, morphological and microscopic characteristics. The rapid and accurate detection of this pathogen will lead to better disease monitoring and control efforts. A loop-mediated isothermal amplification (LAMP) method was then developed to rapidly and specifically identify B. dothidea. These results offer value to further research into kiwifruit ripe rot, such as disease prediction, pathogen rapid detection, and effective disease control.
Nano/Micro Hybrid Bamboo Fibrous Preforms for Robust Biodegradable Fiber Reinforced Plastics
Authors: Bang Junsik , Lee Hyunju , Yang Yemi , Oh Jung-Kwon , Kwak Hyo Won
Published: February 2021

The focus on high-strength and functional natural fiber-based composite materials is growing as interest in developing eco-friendly plastics and sustainable materials increases. An eco-friendly fibrous composite with excellent mechanical properties was prepared by applying the bamboo-derived nano and microfiber multiscale hybridization phenomenon. As a result, the cellulose nanofibers simultaneously coated the micro-bamboo fiber surface and adhered between them. The multiscale hybrid phenomenon implemented between bamboo nano and microfibers improved the tensile strength, elongation, Young’s modulus, and toughness of the fibrous composite. The enhancement of the fibrous preform mechanical properties also affected the reinforcement of biodegradable fiber-reinforced plastic (FRP). This eco-friendly nano/micro fibrous preform can be extensively utilized in reinforced preforms for FRPs and other green plastic industry applications.
GYMEE: A Global Field-Scale Crop Yield and ET Mapper in Google Earth Engine Based on Landsat, Weather, and Soil Data
Authors: Jaafar Hadi , Mourad Roya
Published: February 2021

In this study, we used Landsat Earth observations and gridded weather data along with global soil datasets available in Google Earth Engine (GEE) to estimate crop yield at 30 m resolution. We implemented a remote sensing and evapotranspiration-based light use efficiency model globally and integrated abiotic environmental stressors (temperature, soil moisture, and vapor deficit stressors). The operational model (Global Yield Mapper in Earth Engine (GYMEE)) was validated against actual yield data for three agricultural schemes with different climatic, soil, and management conditions located in Lebanon, Brazil, and Spain. Field-level crop yield data on wheat, potato, and corn for 2015–2020 were used for assessment. The performance of GYMEE was statistically evaluated through root-mean-square error (RMSE), mean absolute error (MAE), mean bias error (MBE), relative error (RE), and index of agreement (d). The results showed that the absolute difference between the modeled and predicted field-level yield was within ±16% for the analyzed crops in both Brazil and Lebanon study sites and within ±15% in the Spain site (except for two fields). GYMEE performed best for wheat crop in Lebanon with a low RMSE (0.6 t/ha), MAE (0.5 t/ha), MBE (−0.06 t/ha), and RE (0.83%). A very good agreement was observed for all analyzed crop yields, with an index of agreement (d) averaging at 0.8 in all studied sites. GYMEE shows potential in providing yield estimates for potato, wheat, and corn yields at a relative error of ±6%. We also quantified and spatialized the soil moisture stress constraint and its impact on reducing biomass production. A showcasing of moisture stress impact on two emphasized fields from the Lebanon site revealed that a 12% difference in soil moisture stress can decrease yield by 17%. A comparison between the 2017 and 2018 seasons for the potato culture of Lebanon showed that the 2017 season with lower abiotic stresses had higher light use efficiency, above-ground biomass, and yield by 5%, 10%, and 9%, respectively. The results show that the model is of high value for assessing global food production.
Low-Cost Sensors and Biological Signals
Authors: Dierick Frédéric , Buisseret Fabien , Eggermont Stéphanie
Published: February 2021

Low-cost sensors, i [...]
Economic Development, Informal Land-Use Practices and Institutional Change in Dongguan, China
Authors: Huang Yingmin , Xue Desheng , Huang Gengzhi
Published: February 2021

This paper is engaged with the critical perspective that highlights the role of the state in the production of urban informality by examining the dynamics of informal land-use practices in Dongguan, China since 1978. Based on in-depth interviews and archival analysis, the relationship between informal land development, the state, and land institution change has been revealed. Our findings show that informal land development is practiced by village collectives from below in Dongguan as a response to the absence and limitation of the national land law. The local government handles the informality in a pragmatic way that serves the goal of economic development in different historical conditions by actions of encouraging, tolerating, and authorizing, suggesting that the definition of informality is not a neutral classification. It is argued that while informality represents people’s creativity in dealing with practical problems, when and to what extent it can be tolerated, formalized, and absorbed depends on the intention of the state in a specific historical context.
An Integrated Approach to Assess Potential and Sustainability of Handmade Carpet Production in Different Areas of the East Azerbaijan Province of Iran
Authors: Pourmoradian Samereh , Vandshoari Ali , Omarzadeh Davoud , Sharifi Ayyoob , Sanobuar Naser , Samad Hosseini Seyyed
Published: February 2021

A handmade carpet is one of the most well-known handcrafts around the world. Iranian handmade carpets are known as luxury products in domestic and international markets due to their strength and product value. The main objective of this research is to apply a geographical information system (GIS)-based, spatially-explicit approach to assess the sustainability of handmade carpet production in the East Azerbaijan Province of Iran, which is internationally famous for the diversity and quality of its handmade carpets. To achieve this goal, we employed 23 criteria in four main clusters: population characteristics, education status, employment status, and business activities related to the carpet industry. In order to determine the significance of each criterion, an integrated approach of fuzzy and network analysis processes was applied. Accordingly, the GIS aggregation function was employed to map and identify the areas that are suitable and of high potential for handmade carpet production. The results indicate that there is a very high potential for handmade carpet production in some areas of Tabriz, Osku, Marageh, Heris, and Meyaneh counties. However, high sustainability also extends to some areas in Marand, Bonab, and Kalaybar counties. The obtained maps present the potential of each city and village for handmade carpet production. The research also aims to evaluate and suggest relevant policies and practices to overcome the identified challenges in order to promote, develop, and preserve the handmade carpet sector. The results of this research are of great importance for different stakeholders in the handmade production and for decision makers and authorities in the East Azerbaijan Province. The results can be used to determine the potential of each area for handmade carpet production and to highlight potential challenges. This research also presents a new approach for sustainability assessments in studies on handcrafts and, in particular, carpets.
Accentuating the Role of Nitrogen to Phosphorus Ratio on the Growth and Yield of Wheat Crop
Authors: Mussarat Maria , Shair Muhammad , Muhammad Dost , Mian Ishaq Ahmad , Khan Shadman , Adnan Muhammad , Fahad Shah , S. Dessoky Eldessoky , EL Sabagh Ayman , Zia Afia , Khan Bushra , Shahzad Haroon , Anwar Shazma , Ilahi Haroon , Ahmad Manzoor , Bibi Hamida , Adnan Muhammad , Khan Farmanullah
Published: February 2021

Nitrogen (N) and Phosphorus (P) deficiency is a major yield limiting factor across the globe and their proper management plays a vital role in optimizing crop yield. This field experiment was conducted to assess the impact of soil and plant nitrogen N and P ratio on the growth and yield of wheat (Triticum aestivum L.) in alkaline calcareous soil. The study consisted of various levels of nitrogen (0, 40, 80, and 160 kg ha−1 as urea) and phosphorus (0, 30, 60, and 90 kg P2O5 ha−1 as diammonium phosphate), and was carried out in randomized complete block design (RCBD) with factorial arrangement having three replications. The result showed that the addition of 160 kg N ha−1 significantly improved biological yield (10,052 kg ha−1), grain weight (3120 kg ha−1), chlorophyll content at tillering stage soil plant analysis development (SPAD) value (35.38), N uptake in straw (33.42 kg ha−1), and K uptake in straw (192 kg ha−1) compared to other N levels. In case of P, 90 kg P2O5 ha−1 had resulted maximum biological yield (9852 kg ha−1), grain yield (3663 kg ha−1), chlorophyll content at tillering stage (SPAD value 34.36), P (6.68 mg kg−1) and K (171 kg ha−1) uptake in straw. The sole use of N and P have positively influenced the biological and grain yield but their interaction didn’t response to biological yield. The present study reveals that SPAD value (chlorophyll meter) is the better choice for determining plant N and P concentrations to estimate the yield potential.
An Analytic Hierarchy Process Approach for Prioritisation of Strategic Objectives of Sustainable Development
Authors: Szabo Zsuzsanna Katalin , Szádoczki Zsombor , Bozóki Sándor , Stănciulescu Gabriela C. , Szabo Dalma
Published: February 2021

Sustainability is one of the world’s fundamental objectives, and a wide variety of information types, parameters, and uncertainties need to be appraised and managed to assess it. In the present paper, Multi-Criteria Decision Analysis (MCDA) is used to prioritise the criteria of sustainable development based on regularly published indicators. In line with most approaches in the literature, the main criteria are Economy, Society and Environment. Complex criteria are decomposed into subcriteria until the performance with respect to them can be measured directly. Weights of importance are calculated by the Analytic Hierarchy Process (AHP), in decision support system PriEsT. The model is flexible to both the modification of criteria and re-weighting, and the PriEsT file is supplemented to the paper. Moreover, the results can also be applied in decisions on resource allocation. The proposed methodology has the potential of resulting in a new composite index to measure, compare or rank countries and regions regarding sustainable development or one of its subcriteria, as well as to track, year by year, the improvements or the impact of the policies introduced.
The Spatial and Mechanism Difference in the Export Evolution of Product Space in Global Countries
Authors: Li Shan , Li Xun , Lang Wei , Chen Haohui , Huang Xiaoguang
Published: February 2021

This study focuses on investigating the changing export patterns, evolution characteristics, and influencing trade mechanisms of countries on a global scale. Based on comprehensive customs data, our study found that core location and export types, including machinery and chemical products, both play positive roles in promoting countries’ economic development. Developed countries are more likely to be at the core of the product space and to export machinery and chemical products. Countries’ R&D investment can affect the export location and types regardless of their economy, while high education matters in developed countries, and FDI (Foreign Direct Investment) is critical in developing countries. It indicates that technological benefits created by human capital can promote the export economy. Nevertheless, developing countries are not able to release strong knowledge spillover effects through their education systems, and they are relying more on the introduction of foreign investment to bring new technology.
Stock Market Reactions to Pollution Information Disclosure: New Evidence from the Pollution Blacklist Program in China
Authors: Zhou Yalin , Cao Jing , Feng Yujia
Published: February 2021

Public disclosure of environmental information has been widely used as an important instrument in green finance. In this paper, we examine a blacklist program of polluting firms and conduct an event study to evaluate how the stock market responds to the pollution news. Our results show that the pollution disclosure indeed had a significant negative effect on the stock market performance of listed companies on the blacklists, but only when the overall market was under downward shocks, suggesting that the shareholders were more sensitive to the pollution news in bad times. When the stock market performed well or was relatively stable, the blacklist effects were not evident. Our heterogeneity analyses further revealed that the magnitude of the cumulative abnormal returns depended on the firm size. That is, the larger the firms are, the less they suffer from the pollution news release. Our findings show that pollution disclosure does penalize the polluting firms through stock market response mechanisms.
Sustainable Tourism Development and Economic Growth: Bibliometric Review and Analysis
Authors: León-Gómez Ana , Ruiz-Palomo Daniel , Fernández-Gámez Manuel A. , García-Revilla Mercedes Raquel
Published: February 2021

Over the past decade, there has been a growing interest in studying the impact of sustainable tourism development on economic growth. However, despite its recent scope, the scientific literature published so far has not evaluated the performance of the scientific activity of this relationship. Consequently, this study analyzes the 668 articles published to date in the Web of Science database on the effect that sustainable tourism development has on the overall long-term progress of the economy. To this end, we carry out an analysis of the most recognized authors, regions with the highest percentage of scientific production, most influential organizations, the co-occurrence of keywords, most prominent citations, publications, and co-authorship among the most recognized authors. The results obtained show the trend and impact of the literature published to date and the established and emerging research groups. Also, they identify key research topics in a way that provides a planning framework for further research in this field.