bluuebunny/crossref_metadata_2025 huggingface dataset:
Co-exposure to lead, mercury, and cadmium induces neurobehavioral impairments in mice by interfering with dopaminergic and serotonergic neurotransmission in the striatum
Authors: Pyatha Sarita , Kim Haesoo , Lee Daeun , Kim Kisok
Published: November 2023

Humans are exposed to lead (Pb), mercury (Hg), and cadmium (Cd) through various routes, including drinking water, and such exposure can lead to a range of toxicological effects. However, few studies have investigated the toxic effects of exposure to mixtures of metals, particularly in relation to neurotoxicity. In this study, 7-week-old male mice were exposed to Pb, Hg, and Cd individually or in combination through their drinking water for 28 days. The mice exposed to the metal mixture exhibited significantly reduced motor coordination and impaired learning and memory abilities compared to the control group and each of the single metal exposure groups, indicating a higher level of neurotoxicity of the metal mixture. The dopamine content in the striatum was significantly lower in the metal mixture exposure group than in the single metal exposure groups and the control group. Furthermore, compared to the control group, the metal mixture exposure group showed a significantly lower expression level of tyrosine hydroxylase (TH) and significantly higher expression levels of dopamine transporter (DAT), tryptophan hydroxylase 1 (TPH1), and serotonin reuptake transporter (SERT). Notably, there were no significant differences in SERT expression between the single metal exposure groups and the control group, but SERT expression was significantly higher in the metal mixture exposure group than in the single metal and control groups. These findings suggest that the key proteins involved in the synthesis and reuptake of dopamine (TH and DAT, respectively), as well as in the synthesis and reuptake of serotonin (TPH1 and SERT, respectively), play crucial roles in the neurotoxic effects associated with exposure to metal mixtures. In conclusion, this study demonstrates that simultaneous exposure to different metals can impact key enzymes involved in dopaminergic and serotonergic neurotransmission processes, leading to disruptions in dopamine and serotonin homeostasis and consequently a range of detrimental neurobehavioral effects.
Assessment of heavy metals among auto workers in metropolitan city: a case study
Authors: Khan Kaleem , Room Shahzada Amani , Bacha Aziz-Ur-Rahim , Nabi Iqra , Ahmad Shabir , Younas Muhammad , Ullah Zahid , Iqbal Akhtar , Alrefaei Abdulwahed Fahad , Almutairi Mikhlid H. , Chang Jung-Wei , Chi Kai Hsien
Published: November 2023

In recent decades, heavy metals (HMs) have emerged as a global health concern. Unfortunately, in Pakistan, there is a general lack of awareness regarding the potential health risks associated with HMs pollution among automobile workers. Herein, we investigated the concentration of heavy metals such as lead (Pb), cadmium (Cd), and chromium (Cr) among automobile workers who were occupationally exposed in Mingora City, Khyber Pakhtunkhwa, Pakistan. Three different automobile groups, i.e., battery recyclers, spray painters, and mechanics were studied in detail. A total of 40 blood samples were collected from automobile workers groups while 10 blood samples were collected as control individuals from different locations in the study area. We investigated heavy metals concentration with a standard method using an atomic absorption spectrometer AAS (PerkinElmer Analyst 700, United States). Based on our findings, the battery recycling group displayed the most elevated Pb levels (5.45 ± 2.11 μg/dL), exceeding those of both the spray painters’ group (5.12 ± 1.98 μg/dL) and the mechanics’ group (3.79 ± 2.21 μg/dL). This can be attributed to their higher exposure to Pb pollution resulting from the deterioration, dismantling, grinding, or crushing of old batteries. In the context of chromium (Cr) exposure, a similar trend was observed among the battery recycling group, as well as the spray painters and mechanics groups. However, in the case of cadmium (Cd), the mechanics’ group exhibited the highest level of exposure (4.45 ± 0.65 μg/dL), surpassing the battery recycling group (1.17 ± 0.45 μg/dL) and the spray painters’ group (1.35 ± 0.69 μg/dL), which was attributed to their greater exposure to welding fumes and other activities in their workplace. We believe that our findings will encourage regulatory measures to improve the health of automobile workers. However, further work is needed to determine various health-related issues associated with heavy metal exposure among automobile workers.
Causal association between air pollution and frailty: a Mendelian randomization study
Authors: Xiao Haixia , Huang Shan , Yang Wei , Zhang Wenni , Xiao Huanshun , Cai Shuangming
Published: November 2023

Backgrounds

Frailty is a significant problem for older persons since it is linked to a number of unfavorable consequences. According to observational researches, air pollution may raise the risk of frailty. We investigated the causal association between frailty and air pollution (including PM2.5, PM2.5–10, PM10, nitrogen dioxide, and nitrogen oxides) using Mendelian randomization approach.

Methods

We conducted MR analysis using extensive publically accessible GWAS (genome-wide association studies) summary data. The inverse variance weighted (IVW) method was employed as the primary analysis method. The weighted median model, MR-Egger, simple model, and weighted model approaches were chosen for quality control. The Cochran’s Q test was utilized to evaluate heterogeneity. Pleiotropy is found using the MR-Egger regression test. The MR-PRESSO method was used to recognize outliers. The leave-one-out strategy was used to conduct the sensitivity analysis.

Results

MR results suggested that PM2.5 was statistically significantly associated with frailty [odds ratio (OR) = 1.33; 95%confidence interval (CI) = 1.12–1.58, p = 0.001] in IVW method. We observed no statistical association between PM2.5–10(OR = 1.00, 95% CI = 0.79–1.28, p = 0.979), PM10(OR = 0.91, 95% CI = 0.75–1.11, p = 0.364), nitrogen dioxide (OR = 0.98, 95% CI = 0.85–1.12, p = 0.730), nitrogen oxides (OR = 1.15, 95% CI = 0.98–1.36, p = 0.086) and frailty. There was no pleiotropy in the results. The sensitivity analysis based on the leave-one-out method showed that the individual single nucleotide polymorphisms (SNPs) did not affect the robustness of the results.

Conclusion

The current MR investigation shows a causal association between PM2.5 and frailty. Frailty’s detrimental progression may be slowed down with the help of air pollution prevention and control.
Change in urban forest age structure affects the value of ecosystem services provided
Authors: Mänttäri Miia M. , Lindén Leena , Tuhkanen Eeva-Maria
Published: November 2023

To achieve resilience goals, urban planners and decision-makers need accurate information on the benefits provided by urban trees and on the effects that management may have on them. This study investigates the impacts of management and disturbances on urban forest structure and function in Turku, Finland. Using a comprehensive urban tree database and the i-Tree software suite, we assessed the current structure and estimated the value of ecosystem services provided by Turku's urban forest. Additionally, we simulated changes in the urban forest over a 50-year period, considering different tree planting scenarios and the potential outbreak of the Asian longhorned beetle (ALB). Turku's urban forest comprised 38,438 public trees, dominated by Acer platanoides, Pinus sylvestris, Tilia × europaea, and Betula pendula. The estimated carbon storage was 12,336 t, valued at 1.98 million €, with an annual sequestration rate of 284 t (45,549 €/year). The trees also removed 8.97 t of pollutants annually, with an estimated value of 153,273 €. At the current rate of tree planting, the number of trees would decline over the course of 50 years resulting in a gradual decrease in the provision of ecosystem services. Although doubling the tree planting rate could slowly increase carbon storage and sequestration even under moderate ALB attack, it was insufficient to offset the damage caused by ALB if tree mortality rate reaches 50%. Compared to carbon storage and sequestration, changes in urban forest age structure had a more immediate impact on the removal of air pollution. These findings emphasize the importance of prioritizing investments in urban forests on grounds of their capacity to provide diverse ecosystem services. Incorporating these findings into decision-making processes would promote sustainable and resilient urban environments.
Challenges faced by the municipal water works management in improving water supply adequacy and distribution in Bontoc, Philippines
Authors: Magwilang Epiphania B. , Paredes Annie Lourie Yawan , Armas Francisco C. , Bugnay Helen Grace P. , Dagupen Rose D.
Published: November 2023

Domestic water is indispensable for daily use, yet its effective management encounters numerous challenges that impact household consumers. This study aims to identify the challenges leading to supply inadequacy and uneven distribution, while proposing interventions to enhance water supply for households. The study employed surveys, interviews, and focus group discussions to gather comprehensive data on domestic water supply issues in rural communities in Bontoc, Philippines. The findings reveal two primary issues in these rural communities: supply inadequacy and unequal distribution. Supply inadequacy is attributed to factors such as wasteful water use, water scarcity during prolonged dry seasons, limited water sources, and population growth. Uneven distribution results from factors like landslides, illegal tapping, irregular water quality, insufficient monitoring of quantity and pressure, and inadequacies in the water distribution network layout. The study suggests several crucial actions for the local government unit (LGU) of Bontoc. These include augmenting water sources, implementing regular water supply monitoring, ensuring timely repairs, replacing old pipes, optimizing distribution pipeline layouts, enhancing water pressure, and rigorously enforcing municipal water ordinances. Furthermore, the study emphasizes the importance of household water management practices, such as responsible consumption, supply conservation, and recycling. The effective implementation of these interventions, through collaboration between the LGU and households, has the potential to ameliorate the constraints in domestic water supply and distribution. This collaborative approach is essential for improving supply management and addressing the current challenges faced by domestic water consumers.
Statistical analysis of equatorial electrojet responses to the transient changes of solar wind conditions
Authors: Zhang Jiawei , Li Qiaoling , Li Shuhan , Liu Jing
Published: November 2023

Introduction: Prior case studies have indicated that changes in solar wind conditions have a significant impact on equatorial ionospheric electrodynamics. However, there have been limited statistical studies on this topic, impairing our understanding of the coupling between solar wind, magnetosphere, and equatorial ionosphere electrodynamics.

Methods: In this study, we conducted a superposed epoch analysis of long-term data from the South America equatorial electrojet (EEJ) spanning from 2001 to 2021 examining the responses of the equatorial ionospheric electric field to step-like changes in solar wind velocity, density, dynamic pressure, and interplanetary magnetic field (IMF) Bz.

Result: Our study shows that step-like changes in solar wind velocity, density, and dynamic pressure can trigger changes in EEJ within ∼20–40 min. EEJ exhibits the highest sensitivity to variations in solar wind velocity while being relatively less sensitive to changes in dynamic pressure. Furthermore, the response of EEJ shows greater responsiveness to northward IMF Bz compared to southward IMF Bz.

Discussion: Our work provides statistical evidence of how changes in solar wind can lead to changes in low-latitude ionospheric EEJ. We inferred that the changes in solar wind conditions cause magnetospheric deformation and changes in magnetic reconnection rates, leading to the fluctuations of the ionospheric electric field and the resultant EEJ variations.
A Recommendation System Supporting the Implementation of Sustainable Risk Management Measures in Airport Operations
Authors: Carpitella Silvia , Brentan Bruno , Certa Antonella , Izquierdo Joaquín
Published: November 2023

This paper introduces a recommendation system aimed at enhancing the sustainable process of risk management within airport operations, with a special focus on Occupational Stress Risks (OSRs). The recommendation system is implemented via a flexible Python code that offers seamless integration into various operational contexts. It leverages Fuzzy Cognitive Maps (FCMs) to conduct comprehensive risk assessments, subsequently generating prioritized recommendations for predefined risk management measures aimed at preventing and/or reducing the most critical OSRs. The system’s reliability has been validated by iterating the procedure with diverse input data (i.e., matrices of varying sizes) and measures. This confirms the system’s effectiveness across a broad spectrum of engineering scenarios.
Efficient and Lightweight Automatic Wheat Counting Method with Observation-Centric SORT for Real-Time Unmanned Aerial Vehicle Surveillance
Authors: Chen Jie , Hu Xiaochun , Lu Jiahao , Chen Yan , Huang Xin
Published: November 2023

The number of wheat ears per unit area is crucial for assessing wheat yield, but automated wheat ear counting still faces significant challenges due to factors like lighting, orientation, and density variations. Departing from most static image analysis methodologies, this study introduces Wheat-FasterYOLO, an efficient real-time model designed to detect, track, and count wheat ears in video sequences. This model uses FasterNet as its foundational feature extraction network, significantly reducing the model’s parameter count and improving the model’s inference speed. We also incorporate deformable convolutions and dynamic sparse attention into the feature extraction network to enhance its ability to capture wheat ear features while reducing the effects of intricate environmental conditions. To address information loss during up-sampling and strengthen the model’s capacity to extract wheat ear features across varying feature map scales, we integrate a path aggregation network (PAN) with the content-aware reassembly of features (CARAFE) up-sampling operator. Furthermore, the incorporation of the Kalman filter-based target-tracking algorithm, Observation-centric SORT (OC-SORT), enables real-time tracking and counting of wheat ears within expansive field settings. Experimental results demonstrate that Wheat-FasterYOLO achieves a mean average precision (mAP) score of 94.01% with a small memory usage of 2.87MB, surpassing popular detectors such as YOLOX and YOLOv7-Tiny. With the integration of OC-SORT, the composite higher order tracking accuracy (HOTA) and counting accuracy reached 60.52% and 91.88%, respectively, while maintaining a frame rate of 92 frames per second (FPS). This technology has promising applications in wheat ear counting tasks.
Effect of Legumes Intercropped with Maize on Biomass Yield and Subsequent Biogas Production
Authors: Kintl Antonín , Huňady Igor , Vítěz Tomáš , Brtnický Martin , Sobotková Julie , Hammerschmiedt Tereza , Vítězová Monika , Holátko Jiří , Smutný Vladimír , Elbl Jakub
Published: November 2023

The presented study deals with the use of legumes intercropped with maize for the production of biogas from silage. The main goal was to find out whether silages made from mixed cultures can be used in biogas production and how the use of such silages affects qualitative and quantitative parameters of the fermentation process compared with the pure maize silage. Variants prepared were pure cultures of maize, bean, lupin, and white sweet clover. In addition, mixed cultures were prepared of maize and individual legumes. Measured values showed that in terms of dry matter (DM) yield, mixed culture silages are almost of the same or even better quality than silage made from the maize monosubstrate. Compared with the maize monoculture silage, the presence of white lupine, white sweet clover, and broad bean in silages statistically significantly increased the content of DM, ash, and acid detergent fiber (by more than 5%). Bean and lupine in mixed silages with maize significantly increased the content of lipids (on average by more than 1.2%). Legumes in silages were significantly decreasing contents of neutral detergent fiber, crude protein, and starch. Production of biogas from silages of maize monosubstrates and mixed substrates of maize with white lupin, maize with white sweet clover, and maize with broad bean was directly proportional to the content of CAR and starch in these substrates. A perspective variant was the mixed substrate of maize and sweet clover from which biogas production was only 6% lower than that from conventional maize silage. The highest yield was recorded in the maize monosubstrate (0.923 m3/kgVS). Variants of mixed substrates had a yield ranging from 0.804 to 0.840 m3/kgVS.
Input Selection Drives Molecular Logic Gate Design
Authors: Souto Francielly T. , Dias Gleiston G.
Published: November 2023

Optical detection devices have become an analytical tool of interest in diverse fields of science. The search for methods to identify and quantify different compounds has transposed this curiosity into a necessity, since some constituents threaten the safety of life in all its forms. In this context, 30 years ago, Prof. Prasanna de Silva presented the idea of sensors as Molecular Logic Gates (MLGs): a molecule that performs a logical operation based on one or more inputs (analytes) resulting in an output (optical modification such as fluorescence or absorption). In this review, we explore the implementation of MLGs based on the interference of a second input (second analyte) in suppressing or even blocking a first input (first analyte), often resulting in INHIBIT-type gates. This approach is interesting because it is not related to attached detecting groups in the MLG but to the relation between the first and the second input. In this sense, flexible and versatile MLGs can be straightforwardly designed based on input selection. To illustrate these cases, we selected examples seeking to diversify the inputs (first analytes and interfering analytes), outputs (turn on, turn off), optical response (fluorescent/colorimetric), and applicability of these MLGs.
Effect of Different Host Plants on Life Type Characteristics of Three Spider Mite Pests (Acari: Prostigmata: Tetranychidae)
Authors: Mushtaq Hafiz Muhammad Saqib , Ali Hafiz Muhammad Sajid , Kamran Muhammad , Alatawi Fahad Jaber
Published: November 2023

The present study evaluated the host plant effect on life type characteristics of three important spider mite pest species, Tetranychus urticae Koch, Eutetranychus orientalis (Klein), and E. palmatus Attiah (Acari: Prostigmata: Tetranychidae), based on both field and laboratory observations. The polyphagous species, T. urticae with complicated web (CW-u) life type, occupying unstable habitats, showed variations in the sites for quiescence (SQ), sites for oviposition (SO), sites for defecation (SD), and webbing density (WD) on different annual/perennial host plants. The SQ, SO, and SD of T. urticae were observed either on the leaf, web threads, or trichomes. Tetranychus urticae constructed the lowest WD on tomato plants and the highest WD on maize/mulberry plants. Two spider mite species of the genus Eutetranychus Banks, the polyphagous E. orientalis and the oligophagous E. palmatus, inhabit stable host plants, depicted in the little web (LW-j) life types with persistency in all characteristics on different plants. It is concluded that polyphagous spider mites have restricted their life types, showing their high adaptability to utilize the resources of different host plants for survival with slight variation in some important life type characteristics.
Effects of Hemp Seed on the Production, Fatty Acid Profile, and Antioxidant Capacity of Milk from Goats Fed Hay or a Mixed Shrubs–Grass Rangeland
Authors: Mierlita Daniel , Mierlita Stefania , Struti Danut Ioan , Mintas Olimpia Smaranda
Published: November 2023

The research objective was to evaluate the effect of dietary incorporation of hemp seeds in goats fed with hay or mixed shrubs–grass rangeland on the production, FA profile and health-related lipid indices, antioxidant content and total antioxidant capacity of milk, with the purpose to increase the content of beneficial ingredients in milk and to improve its functional activity. Forty indigenous Carpathian goats were allocated into two groups according to the type of basic forage in their diet: hay (H) or mixed shrubs–grass rangeland (SG); each of them was further divided into two subgroups according to the presence of Hs in the concentrate mixture (250 g/kg) or not. Milk production was determined, and milk samples were collected and analyzed for fat, protein, lactose, and cholesterol content, as well as FA profile, lipophilic antioxidant content (α-tocopherol and retinol), and milk TAC. SG goats gave less milk (p < 0.01) but with higher milk fat (p < 0.001) and lower cholesterol content (p < 0.01) than H goats, while milk protein and lactose contents were not affected. Supplementing the diet with Hs caused a significant increase in milk production (p < 0.05) and milk fat content (p < 0.001) and a decrease in cholesterol content (p < 0.05). Grazing compared to indoor feeding but also supplementing the diet with Hs had the effect of decreasing the proportion of SFAs and increasing the concentrations of polyunsaturated FA (PUFA) in goat milk fat (p < 0.01). Fats in the milk of SG goats compared to H, but also in those supplemented with Hs, showed significantly higher proportions of vaccenic acid (VA), rumenic acid (CLA c-9,t-11) and omega-3 FA (α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosapentaenoic acid (DPA)) which are considered healthy for consumers. The feeding system based on SG and the diet supplementation with Hs ensured the best nutritional and functional quality of milk, confirmed by the FA profile, antioxidant content, and by the value of health-related lipid indices (n-6/n-3 FA ratio and hypo-/hypercholesterolemia, atherogenic index (AI), thrombogenic index (TI), and health promotion index (HPI)). The results of our work will be useful for the development of optimal nutritional strategies that improve the FA profile and the antioxidants content in goat milk, with beneficial effects on human health.
Endothelial Notch Signaling Regulates the Function of the Retinal Pigment Epithelial Barrier via EC Angiocrine Signaling
Authors: Niu Yali , Xi Yixuan , Jing Yutong , Zhou Ziyi , Sun Xiaojia , Zhang Guoheng , Yuan Tianhao , Chang Tianfang , Dou Guorui
Published: November 2023

The outer blood–retina barrier (oBRB), comprises tightly connected retinal pigment epithelium (RPE) cells, Bruch’s membrane, and choroid blood vessels, and is essential for retinal health and normal visual function. Disruption of the RPE barrier and its dysfunction can lead to retinal disorders such as age-related macular degeneration (AMD). In the present study, we investigated the essential role of choroid endothelial cells (ECs) in the RPE barrier formation process and its dysfunction. We discovered that ECs promoted RPE barrier formation through angiocrine signaling. Through blocking or activating endothelial Notch signaling and conducting experiments in vitro and in vivo, we confirmed that endothelial Notch signaling regulated the expression of heparin-binding epidermal growth factor (HBEGF) and consequently impacted the expression and activity of matrix metalloproteinases (MMP)-9 in RPE cells. This modulation influenced the RPE extracellular matrix deposition, tight junctions and RPE barrier function. In in vivo experiments, the intravitreal administration of recombinant HBEGF (r-HBEGF) alleviated the RPE barrier disruption induced by subretinal injection (SI) or laser treatment and also rescued RPE barrier disruption in endothelial Notch-deficient mice. Our results showed that the endothelial Notch signaling drove HBEGF expression through angiocrine signaling and effectively improved RPE barrier function by regulating the MMP-9 expression in RPE cells. It suggests that the modulation of Notch signaling in the choroidal endothelium may offer a novel therapeutic strategy for retinal degenerative diseases.
Effect of Sonication Associated with Pasteurization on the Inactivation of Methicillin-Resistant Staphylococcus aureus in Milk Cream
Authors: Nascimento Joselene Conceição Nunes , Salgado Madian Johel Galo , Gutierrez Alzate Katherine , de Alencar Joseane Cardoso Gomes , Rosario Iuri Lima dos Santos , da Silva José Givanildo , Paulino Bruno Nicolau , da Costa Marion Pereira
Published: November 2023

Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant challenge to the dairy industry, necessitating robust strategies to ensure food safety. This study focuses on the efficacy of thermosonication, a novel technology combining ultrasound and heat, in reducing MRSA in milk cream. Comparative analysis is conducted with conventional pasteurization, the industry standard. Results indicate that thermosonication effectively reduces MRSA counts by up to 4.72 log CFU/mL, akin to pasteurization’s reduction of 4.82 log CFU/mL. This finding highlights the potential of thermosonication as a rapid, energy-efficient alternative to pasteurization in the dairy industry, significantly reducing processing time while maintaining microbial safety. Further exploration and optimization of these techniques promise enhanced food safety and quality control in dairy products, addressing the growing concern of antibiotic-resistant strains like MRSA. This research lays a foundation for innovative approaches and underscores the significance of quantitative data in food safety research.
Fracture Network Analysis of Karstified Subis Limestone Build-Up in Niah, Sarawak
Authors: Ong Poh Yee , Jamaludin Siti Nur Fathiyah
Published: November 2023

Understanding complex carbonate fracture networks and karstification at various geological scales is challenging, especially with limited multi-scale datasets. This paper aims to reduce uncertainty in the fracture architecture of Central Luconia karstified reservoirs by narrowing observational gaps between seismic and well data by using the discrete fracture models of exposed limestone outcrops as analogues for the subsurface carbonate reservoir. An outcrop-based fracture network characterisation of a near-surface paleo-karst at Subis Limestone combined with lineament analysis was conducted to extract fracture parameters. The karst structure was first delineated using a digital elevation map and outcrop examination. Then, topology analysis was performed, following the creation of two-dimensional discrete fracture models. Two main fracture sets oriented northeast–southwest and northwest–southeast and 79 potential dolines were identified. Fracture intersections, northeast–southwest major orientations, and drainage systems highly influenced the karst features. The Subis Limestone fracture model revealed that the highest number of fractures and total length of fractures were concentrated in the northern part of the Subis Limestone build-up (X: 250–350, Y: 150–250) and became denser towards the northwest direction of the outcrop (X: 600–800). The fractures in the Subis paleo-karsts appear isolated, with I-nodes ranging from 0.74 to 0.94. Hence, it is crucial to incorporate matrix porosity into multiple scales of fracture network modelling to improve upscaling and the modelling of fracture–vug networks, as well as to minimise the underestimation of discrete fracture networks in fractured and karstified limestone.
Sampling-Based Path Planning Algorithm for a Plug & Produce Environment
Authors: Ramasamy Sudha , Eriksson Kristina M. , Danielsson Fredrik , Ericsson Mikael
Published: November 2023

The purpose of this article is to investigate a suitable path planning algorithm for a multi-agent-based Plug & Produce system that can run online during manufacturing. This is needed since in such systems, resources can move around frequently, making it hard to manually create robot paths. To find a suitable algorithm and verify that it can be used online in a Plug & Produce system, a comparative study between various existing sampling-based path planning algorithms was conducted. Much research exists on path planning carried out offline; however, not so much is performed in online path planning. The specific requirements for Plug & Produce are to generate a path fast enough to eliminate manufacturing delays, to make the path energy efficient, and that it run fast enough to complete the task. The paths are generated in a simulation environment and the generated paths are tested for robot configuration errors and errors due to the target being out of reach. The error-free generated paths are then tested on an industrial test bed environment, and the energy consumed by each path was measured and validated with an energy meter. The results show that all the implemented optimal sampling-based algorithms can be used for some scenarios, but that adaptive RRT and adaptive RRT* are more suitable for online applications in multi-agent systems (MAS) due to a faster generation of paths, even though the environment has more constraints. For each generated path the computational time of the algorithm, move-along time and energy consumed are measured, evaluated, compared, and presented in the article.
A New Model for Cleaning Small Cuttings in Extended-Reach Drilling Based on Dimensional Analysis
Authors: Jin Liping , Wang Gan , Deng Jingen , Li Ze , Zhu Mingchi , Wang Rong
Published: November 2023

A wellbore’s cleanliness affects drilling efficiency, economy and drilling safety directly in extended-reach drilling operations. Wellbore cleaning in extended-reach drilling has always been a tough problem. Field experience has shown that inefficient transport of small cuttings is a main factor for excessive drag and torque during extended-reach drilling. However, very little is known about the transport of small cuttings. In this paper, we use the data fitting method of dimensional analysis and regression analysis to establish a wellbore cleaning model for high-inclination sections, then use water and Polyanionic Cellulose (PAC) as a drilling fluid to analyze the settlement cleaning of small cuttings in the wellbore. The data from the example wells were used for field simulations to finally derive many factors like drilling fluid density, drilling fluid displacement, drill pipe rotation speed, diameter of cuttings and annulus hydraulic diameter, which affect the thickness of the dimensionless cutting bed. The results show that the dimensionless cutting thickness decreases with increasing drilling fluid density, drilling fluid displacement and drill pipe rotation speed; and increases with increasing cutting size and annular hydraulics diameter. Meanwhile, the effect of drilling fluid discharge, drill pipe rotation speed and cutting size on the thickness of the dimensionless cutting bed decreases as their values increase. This model can be utilized to design parameters in drilling and to predict the transport of cuttings in high-inclination sections. The successful establishment of the wellbore cleaning model for small cuttings in high-inclination sections of extended-reach drilling wells is highly innovative, which is a successful combination of theory and practice and has important guiding significance for promoting the development of wellbore cleaning technology.
War and Contemporary Georgian Theatre
Authors: Chkhartishvili Lasha
Published: November 2023

How is war and its consequences reflected in the theatre? How, in particular, has the Georgian theatre reacted to war, and to what degree does its presence impact Georgian theatre directors and audiences? When and under what circumstances do theatre companies stage plays on the theme of war? Since war never loses its relevance for Georgians, new texts are written continually on this topic and subsequently turned into plays, primarily by young directors. These productions grapple with the experience of war and its impact on the nation and its people, who are radically transformed by their individual histories. In addition, sometimes, they help to reveal hidden passions.
Enhancement of Repeat-Mediated Deletion Rearrangement Induced by Particle Irradiation in a RecA-Dependent Manner in Escherichia coli
Authors: Hou Zhiyang , Xu Zelin , Wu Mengying , Ma Liqiu , Sui Li , Bian Po , Wang Ting
Published: November 2023

Repeat-mediated deletion (RMD) rearrangement is a major source of genome instability and can be deleterious to the organism, whereby the intervening sequence between two repeats is deleted along with one of the repeats. RMD rearrangement is likely induced by DNA double-strand breaks (DSBs); however, it is unclear how the complexity of DSBs influences RMD rearrangement. Here, a transgenic Escherichia coli strain K12 MG1655 with a lacI repeat-controlled amp activation was used while taking advantage of particle irradiation, such as proton and carbon irradiation, to generate different complexities of DSBs. Our research confirmed the enhancement of RMD under proton and carbon irradiation and revealed a positive correlation between RMD enhancement and LET. In addition, RMD enhancement could be suppressed by an intermolecular homologous sequence, which was regulated by its composition and length. Meanwhile, RMD enhancement was significantly stimulated by exogenous λ-Red recombinase. Further results investigating its mechanisms showed that the enhancement of RMD, induced by particle irradiation, occurred in a RecA-dependent manner. Our finding has a significant impact on the understanding of RMD rearrangement and provides some clues for elucidating the repair process and possible outcomes of complex DNA damage.
Relationship between Copper, Zinc, and Copper-to-Zinc Ratio in Hair and Severity of Coronary Artery Disease according to the SYNTAX Score
Authors: Dziedzic Ewelina A. , Gąsior Jakub S. , Tuzimek Agnieszka , Czestkowska Ewa , Beck Joanna , Jaczewska Beata , Zgnilec Elżbieta , Osiecki Andrzej , Kwaśny Mirosław , Dąbrowski Marek J. , Kochman Wacław
Published: November 2023

Coronary artery disease (CAD) continues to be a foremost contributor to global mortality, and the quest for modifiable risk factors could improve prophylactic strategies. Recent studies suggest a significant role of zinc (Zn) and copper (Cu) deficiency in atheromatous plaque formation. Furthermore, hair was previously described as a valuable source of information on elemental burden during the 6–8 week period before sampling. The aim of this study was to investigate the possibility of correlation between the extent of CAD evaluated with the SYNergy Between PCI With TAXUS and the Cardiac Surgery (SYNTAX) score with Cu and Zn content in hair samples, as well as with the Cu/Zn ratio in a cohort of 130 patients. Our findings describe a statistically significant inverse correlation between Cu content and the Cu/Zn ratio in hair samples and the extent of CAD. In contrast, no significant correlation was found between Zn content and the extent of CAD. Considering the scarcity of existing data on the subject, the analysis of hair samples could yield a novel insight into elemental deficiencies and their potential influence on CAD extent.
Plant Foliar Geometry as a Biomimetic Template for Antenna Design
Authors: Lozano Jose Ignacio , Panduro Marco A. , Méndez-Alonzo Rodrigo , Alonso-Arevalo Miguel A. , Conte Roberto , Reyna Alberto
Published: November 2023

Plant diversity includes over 300,000 species, and leaf structure is one of the main targets of selection, being highly variable in shape and size. On the other hand, the optimization of antenna design has no unique solution to satisfy the current range of applications. We analyzed the foliar geometries of 100 plant species and applied them as a biomimetic design template for microstrip patch antenna systems. From this set, a subset of seven species were further analyzed, including species from tropical and temperate forests across the phylogeny of the Angiosperms. Foliar geometry per species was processed by image processing analyses, and the resultant geometries were used in simulations of the reflection coefficients and the radiation patterns via finite differences methods. A value below −10 dB is set for the reflection coefficient to determine the operation frequencies of all antenna elements. All species showed between 3 and 15 operational frequencies, and four species had operational frequencies that included the 2.4 and 5 GHz bands. The reflection coefficients and the radiation patterns in most of the designs were equal or superior to those of conventional antennas, with several species showing multiband effects and omnidirectional radiation. We demonstrate that plant structures can be used as a biomimetic tool in designing microstrip antenna for a wide range of applications.
A Novel Combination Therapy Tβ4/VIP Protects against Hyperglycemia-Induced Changes in Human Corneal Epithelial Cells
Authors: Ebrahim Abdul Shukkur , Carion Thomas W. , Ebrahim Thanzeela , Win Jeff , Kani Hussein , Wang Yuxin , Stambersky Ashten , Ibrahim Ahmed S. , Sosne Gabriel , Berger Elizabeth A.
Published: November 2023

Despite the prevalence of diabetic retinopathy, the majority of adult diabetic patients develop visually debilitating corneal complications, including impaired wound healing. Unfortunately, there is limited treatment for diabetes-induced corneal damage. The current project investigates a novel, peptide-based combination therapy, thymosin beta-4 and vasoactive intestinal peptide (Tβ4/VIP), against high-glucose-induced damage to the corneal epithelium. Electric cell–substrate impedance sensing (ECIS) was used for real-time monitoring of barrier function and wound healing of human corneal epithelial cells maintained in either normal glucose (5 mM) or high glucose (25 mM) ± Tβ4 (0.1%) and VIP (5 nM). Barrier integrity was assessed by resistance, impedance, and capacitance measurements. For the wound healing assay, cell migration was also monitored. Corneal epithelial tight junction proteins (ZO-1, ZO-2, occludin, and claudin-1) were assessed to confirm our findings. Barrier integrity and wound healing were significantly impaired under high-glucose conditions. However, barrier function and cell migration significantly improved with Tβ4/VIP treatment. These findings were supported by high-glucose-induced downregulation of tight junction proteins that were effectively maintained similar to normal levels when treated with Tβ4/VIP. These results strongly support the premise that Tβ4 and VIP work synergistically to protect corneal epithelial cells against hyperglycemia-induced damage. In addition, this work highlights the potential for significant translational impact regarding the treatment of diabetic patients and associated complications of the cornea.
MiRNA-Responsive CRISPR-Cas System via a DNA Regulator
Authors: Yun Dayoung , Jung Cheulhee
Published: November 2023

Clustered regularly interspaced short palindromic repeats (CRISPR)- CRISPR-associated protein 9 (Cas9) genome editing technology is widely used for gene editing because it provides versatility in genetic manipulation. Several methods for regulating CRISPR activity already exist for accurate editing, but these require complex engineering. Thus, a simple and convenient regulatory system is required. In this study, we devised a CRISPR activation system using a DNA regulator that can be activated by miRNAs. The designed regulator was divided into two parts. The inhibition component consisted of the protospacer-adjacent motif (PAM) and seed sequence, which are important for Cas9 target recognition and bind to the ribonucleoprotein (RNP) complex for inhibition. The miRNA recognition component has a single-stranded toehold DNA for target miRNA binding and a partial double-stranded DNA complementary to the remaining miRNA sequence. In the presence of target miRNAs, the structure of the regulator is disrupted by the miRNAs, leading to its dissociation from the RNP complex and subsequent restoration of CRISPR activity. This method is easy to design and can be applied to various miRNAs via simple sequence manipulation. Therefore, this strategy provides a general platform for controlled genome editing.
Poor Oral Health Linked with Higher Risk of Alzheimer’s Disease
Authors: Kulkarni Mihir S. , Miller Brandi C. , Mahani Manan , Mhaskar Rahul , Tsalatsanis Athanasios , Jain Shalini , Yadav Hariom
Published: November 2023

Alzheimer’s disease (AD) is a multifactorial neurodegenerative disease characterized by cognitive and behavioral changes in older adults. Emerging evidence suggests poor oral health is associated with AD, but there is a lack of large-scale clinical studies demonstrating this link. Herein, we used the TriNetX database to generate clinical cohorts and assess the risk of AD and survival among >30 million de-identified subjects with normal oral health (n = 31,418,814) and poor oral health (n = 1,232,751). There was a greater than two-fold increase in AD risk in the poor oral health cohort compared to the normal oral health group (risk ratio (RR): 2.363, (95% confidence interval: 2.326, 2.401)). To reduce potential bias, we performed retrospective propensity score matching for age, gender, and multiple laboratory measures. After matching, the cohorts had no significant differences in survival probability. Furthermore, when comparing multiple oral conditions, diseases related to tooth loss were the most significant risk factor for AD (RR: 3.186, (95% CI: 3.007, 3.376)). Our results suggest that oral health may be important in AD risk, regardless of age, gender, or laboratory measures. However, more large-scale cohort studies are necessary to validate these findings and further evaluate links between oral health and AD.
Electrophysiological Correlates of Vocal Emotional Processing in Musicians and Non-Musicians
Authors: Nussbaum Christine , Schirmer Annett , Schweinberger Stefan R.
Published: November 2023

Musicians outperform non-musicians in vocal emotion recognition, but the underlying mechanisms are still debated. Behavioral measures highlight the importance of auditory sensitivity towards emotional voice cues. However, it remains unclear whether and how this group difference is reflected at the brain level. Here, we compared event-related potentials (ERPs) to acoustically manipulated voices between musicians (n = 39) and non-musicians (n = 39). We used parameter-specific voice morphing to create and present vocal stimuli that conveyed happiness, fear, pleasure, or sadness, either in all acoustic cues or selectively in either pitch contour (F0) or timbre. Although the fronto-central P200 (150–250 ms) and N400 (300–500 ms) components were modulated by pitch and timbre, differences between musicians and non-musicians appeared only for a centro-parietal late positive potential (500–1000 ms). Thus, this study does not support an early auditory specialization in musicians but suggests instead that musicality affects the manner in which listeners use acoustic voice cues during later, controlled aspects of emotion evaluation.
Annual Comparison of the Atmospheric Urban Heat Island in Rome (Italy): An Assessment in Space and Time
Authors: De Cristo Edoardo , Evangelisti Luca , Battista Gabriele , Guattari Claudia , De Lieto Vollaro Roberto , Asdrubali Francesco
Published: November 2023

This study examined the atmospheric urban heat island (UHI) phenomenon within the city of Rome (Italy) and its effects on building energy demand. Weather data from 2020 and 2022 collected from six meteorological stations were considered. A Geographic Information System (GIS) was used to analyze the landscape, correlating the percentage of impermeable surfaces with UHI intensity values in each area. Dynamic simulations were conducted using different climatic data to estimate the heating and cooling energy demands for two representative residential buildings. The findings revealed significant differences in the climatic conditions between urban and rural areas, primarily due to temperature increases. The UHI intensities reached maximum values of 4.67 °C and 3.54 °C in 2020 and 2022. In urban areas, the UHI has positive effects on the heating energy demand but results in a significant increase in energy demand for cooling. Considering a building type constructed between 1900 and 1950, a variation of up to 33.03% in the heating energy demand in urban areas compared to rural areas was calculated, along with a variation of up to 81% for cooling. In contrast, considering a more recent building type constructed between 1991 and 2005, the corresponding values reached up to 36.47% and 75.7%.
Damage Behavior Study of Specimens with Double-Prefabricated Cracks under Dynamic–Static Coupling Loads
Authors: Guo Yujing , Xiong Ziming , Li Zhihao , Yan Fuhuai , Cheng Xiangzhen , Zhang Junnan
Published: November 2023

The surrounding rock of the deep-buried chamber contains high-ground stress and initial cracks. Under a dynamic load, cracks will develop and expand, leading to the fracture and collapse of the confining pressure. Therefore, it is essential to study the failure process of fissured surrounding rock under the joint action of static stress and a dynamic load. In this paper, samples with cracks are used to simulate the defective rock mass. Similar modeling tests and numerical simulation studies were carried out to reveal the damage process of cracked deep rock mass under dynamic disturbance and investigate the impact threshold of rock mass damage under a certain level of hydrostatic pressure. The model test investigated the damage behavior for specimens with double-prefabrication cracks under pressure from a dynamic–static coupling load. The influence of the mechanisms of the angle of a crack, the initial static pressure, and impact capacity on specimen damage was analyzed. It was perceived that, with an increase in the angle of the crack, the omen of specimen damage is less obvious, and the specimen is subjected to sudden damage. On this basis, the damage process of the specimen containing prefabricated cracks under combined dynamic and static loads is realized through numerical simulation, and tests verify the accuracy of the results. The analysis allowed us to come up with a variation rule for the single-disturbance energy threshold for specimens with a prefabricated crack angle and the initial static load level of the specimen containing double-prefabrication cracks. The study lays the foundation for the future analysis of any deep rock mass failure process under dynamic disturbance and the protection of a deeply buried chamber.