bluuebunny/crossref_metadata_2025 huggingface dataset:
Lessons from two series by physicians and caregivers’ self-reported data, and DNA methylation profile in DDX3X-Related Disorders
Authors: Geneviève David , Ruault Valentin , Burger Pauline , Gradels-Hauguel Johanna , Ruiz-Pallares Nathalie , Association Xtraordinaire , Jamra Rami Abou , Afenjar Alexandra , Alembik Yves , Alessandri Jean-Luc , Stéphanie Arpin , Barcia Giulia , Bendová Šárka , Bruel Ange-Line , Charles Perrine , Chatron Nicolas , Chopra Maya , Conrad Solène , Cormier-Daire Valérie , Cospain Auriane , Coubes Christine , Coursimault Juliette , Delahaye-Duriez Andrée , Doco-Fenzy Martine , Dufour William , Durand Benjamin , ENGEL Camille , Faivre Laurence , Ferroul Fanny , FRADIN Mélanie , Frenkiel Hélène , Fusco Carlo , Garavelli Livia , Garde Aurore , Gérard Bénédicte , Germanaud David , Goujon Louise , Gouronc Aurélie , Ginglinger Emmanuelle , Goldenberg Alice , Hancarova Miroslava , Hancarova Miroslava , Héron Delphine , Isidor Bertrand , Marçais Nolwenn Jean , Keren Boris , Koch-Hogrebe Margarete , Kuentz Paul , Lamure Victoria , Lebre Anne-Sophie , Lecoquierre François , Lehman Natacha , Lesca Gaetan , Lyonnet Stanislas , Martin Delphine , Mignot Cyril , Neuhann Teresa , Nicolas Gaël , Nizon Mathilde , Petit Florence , Philippe Christophe , Piton Amélie , Pollazzon Marzia , Prchalova Darina , Putoux Audrey , RIO Marlène , Rondeau Sophie , Rossi Massimiliano , Sabbagh Quentin , Saugier-Veber Pascale , Schmetz Ariane , Steffann Julie , Thauvin-Robinet Christel , Toutain Annick , Tran-Mau-Them Frédéric , Trimarchi Gabriele , Vincent Marie , Vlckova Marketa , Wieczorek Dagmar , Willems Marjolaine , yauy kevin , Zelinová Michaela , Ziegler Alban , Chaumette Boris , Sadikovic Bekim , Mandel Jean-Louis
Published: April 2023

We report two series of individuals with DDX3X variations, one (48 individuals) from physicians and one (44 individuals) from caregivers. These two series include several symptoms in common, with fairly similar distribution, which suggests that caregivers’ data are close to physicians’ data. For example, both series identified early childhood symptoms that were not previously described: feeding difficulties, mean walking age and age at first words. Each of the two datasets provide complementary knowledge. We confirmed that symptoms are similar to those in the literature and provide more details on feeding difficulties. Caregivers considered that the symptom attention-deficit/hyperactivity disorder was most worrisome. Both series also reported sleep disturbance. Recently, anxiety has been reported in individuals with DDX3X variants. We strongly suggest that attention-deficit/hyperactivity disorder, anxiety and sleep disorders need to be treated. In addition, we demonstrate preliminary evidence of a mild genome-wide DNA methylation profile in patients carrying mutations in DDX3X.
Image restoration technology of Tang Dynasty tomb murals based on adversarial edge learning
Authors: Yan Yao , Zhang Rui , He Hao , Lei Tong , Ma Li , Wang Wei , Jiang Chao
Published: April 2023

Xi'an, China houses a vast collection of valuable Tang dynasty (618AD-907AD) tomb murals that have experienced various degrees of damage over time due to weathering. This research proposes an adversarial edge learning-based mural restoration technique that provides a fast and accurate automatic repair of the murals. The method uses generative adversarial networks to learn how to repair the edge contour of the damaged mural and restore the missing content. The contour restoration network and the mural restoration network are trained and updated simultaneously to enhance the feature extraction and restoration coordination performance of the network. The experiments were conducted on a self-built Tang dynasty tomb mural painting restoration dataset and compared to several existing algorithms. The proposed algorithm showed a 1.3 increase in Peak Signal to Noise Ratio (PSNR) and a 1.43% increase in Structural Similarity (SSIM) value, providing a precise restoration of the structure and contour of the mural content. The algorithm successfully repaired damaged murals with fractures and small parts breaking off. The suggested method can be useful for both the technical repair and conservation of significant national visual cultural property and the digital restoration of historic murals.
Enhancing the nutritional management of boron in oil palm crops through a comprehensive evaluation of its dynamics in different plant organs
Authors: Viégas Ismael de Jesus Matos , Silva Washington Duarte Silva da , Ferreira Eric Victor de Oliveira , Pereira Greice Leal , Costa Milton Garcia , Silva Alasse Oliveira da , Conceição Heráclito Eugênio Oliveira da
Published: April 2023

This study aimed to evaluate the rates of B accumulation, immobilization, recycling, and exportation in oil palm plants at different cultivation ages and in various organs. The study was conducted in oil palm plantations owned by Agropalma S/A in the municipality of Tailândia, Pará State, Brazil. A completely randomized design was used, with four replications and seven treatments, representing plant ages ranging from 2 to 8 years of planting. The dynamics of B were evaluated in different plant organs, including petioles, rachis, leaflets, cabbage (palm heart), stipe, peduncles, arrows, male inflorescences, spikelets, and fruits. The findings showed that cabbage had the highest B concentration in the vegetative organs, while male inflorescences had the highest levels in the reproductive organs. Notably, stipe and fruits were the vegetative and reproductive organs with the highest B accumulation, which increased with plant age. In older plants, the largest amount of B exported occurred in bunches. Furthermore, the rates of B immobilization and recycling were higher than the amounts of B exported by oil palm trees. The results of this study have important implications for the nutritional management of B in oil palm crops. Given that B is the most limiting micronutrient in palm oil crop in the Amazon. In conclusion, this study provides a comprehensive understanding of the dynamics of B in oil palm plants, which could help unlock their full potential and promote sustainable crop management. This research highlights the importance of nutrient management to increase crop yield and sustainability.
Genome sequence and characterization of a novel ourmia-like virus infecting Botryosphaeria dothidea in China
Authors: Song Xinzheng , Wang Yanfen , Li Changshui , Xie Shunpei , Yin Xinming , Guo Yashuang , Geng Yuehua , Guo Lihua , Wu Haiyan , Zhang Meng
Published: April 2023

Here, we describe the complete genome sequence and molecular characterization of a novel ourmia-like virus, tentatively named “Botryosphaeria dothidea ourmia-like virus 3” (BdOLV3), isolated from the phytopathogenic fungus Botryosphaeria dothidea strain ZM190712-1, associated with branch dieback of walnut in Henan Province, China. The complete BdOLV3 genome is 2247 nucleotides (nt) in length, with a content of 31.55% G, 16.11% A, 23.54% U, and 28.79% C. The BdOLV3 genome contains a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) of 612 amino acids (aa) with a molecular mass of 67.68 kDa. This RdRp protein contains a catalytic core domain associated with ourmia-like virus in the family Botourmiaviridae. A phylogenetic tree revealed that the novel ourmia-like virus clustered with members of the genus Magoulivirus and was separate from members of the other eleven genera of the family Botourmiaviridae.
Effect of climate change on stratification of a small, monomictic, temperate, karstic lake (Lake Kozjak, Croatia)
Authors: Šarović Kristina , Klaić Zvjezdana Bencetić
Published: April 2023

As delicate closed systems, lakes are extremely vulnerable to climate change. In this paper, we examine Lake Kozjak, Croatia, a small karstic, monomictic lake that belongs to the Plitvice Lake system. The Plitvice Lakes represent a special hydrogeological karstic phenomenon that is closely dependent on a delicate biochemical balance necessary for tufa formation. We apply a simple one-dimensional model for monomictic lakes, SIMO v.1.0, to predict the water temperature at Lake Kozjak in three future scenarios (RCP2.6, RCP4.5 and RCP8.5) for the period between 2006 and 2100. The model captures the seasonal stratification and deepening of the thermocline as well as convective mixing. The results show that the significant increase in the lake water temperature that is expected in the more pessimistic scenarios is not accompanied by significant stratification strengthening due to significant heating of not only the epilimnion but also the hypolimnion. However, significant lengthening of the stratification period is observed even for the most stringent scenario, RCP2.6.
Predicting phenotype to mechanotype relationships in cells based on intra-cellular signaling network
Authors: Katira Parag , Karabay Esra , Turnlund Amy , Grear Jessica , Fraley Stephanie
Published: April 2023

Cells originating from the same tissue can respond differently to external signals depending on the genotypic and phenotypic state of the cell and its local environment. We have developed a semi-quantitative-computational model to analyze the intracellular signaling network and its outcome in the presence of multiple external signals including growth factors, hormones, and extracellular matrix. We use this model to analyze the cell’s mechanical response to external stimuli and identify the key internal elements of the network that drive specific outcomes within the response space. The model is built upon the Boolean approach to network modeling, where the state of any given node is determined using the state of the connecting nodes and Boolean logic. This allows us to analyze the network behavior without the need to estimate all the various interaction rates between different cellular components. However, such an approach is limited in its ability to predict network dynamics and temporal evolution of the cell state. So, we introduce modularity in the model and incorporate dynamical aspects, mass-action kinetics, and chemo-mechanical effects on only certain transition rates within specific modules as required, creating a Boolean-Hybrid-Modular (BoHyM) signal transduction model. We present this model as a comprehensive, cell-type agnostic, user-modifiable tool to investigate how extra-and intra-cellular signaling can regulate cellular cytoskeletal components and consequently influence cell-substrate interactions, force generation, and migration. Using this tool, we show how slight changes in signaling network architectures due to phenotypic changes can alter cellular response to stress hormone signaling in an environment-dependent manner. The tool also allows isolating effector proteins driving specific cellular mechanical responses. Ultimately, we show the utility of the tool in analyzing transient chemo-mechanical dynamics of cells in response to time-varying chemical stimuli.
Ecological and evolutionary success in a parthenogenetic praying mantis: population genetics and reproductive strategy
Authors: Hurd Lawrence , Cooper Gregory J. , Cabe Paul R.
Published: April 2023

Brunneria borealis, a praying mantid endemic to North America, is wingless, and among more than 2400 worldwide is the only one consisting entirely of parthenogenetic females. In spite of the putative handicaps associated with low vagility and parthenogenetic reduction in genetic variation, this mantid is both abundant and widely distributed along the coastline of North America from eastern Texas to North Carolina. We sampled populations across the mantid’s geographic range to discover clues to its ecological and evolutionary success: (1) the specific genetic mechanism of parthenogenesis, and (2) reproductive output. We found no variation in the mitochondrial COI gene among specimens collected over the extensive geographic range, suggesting recent establishment of this range. Surprisingly, observed heterozygosity in nuclear DNA was higher than expected for most loci, and together with SNP data suggest automictic thelytoky with central fusion for populations originating recently from a single individual. Reproduction was remarkable for its long duration: oviposition of multiple oothecae from a single female (up to six oothecae over 56 days), and time between successive emergences of nymphs from individual oothecae (mean = 31.6+4.75 days) would spread out life history stages in a population, potentially mitigating low egg viability (mean = 14+4.0% of eggs hatched per ootheca) by ensuring survival in a seasonal environment with temporally irregular availability of suitable prey.
Distributed Out-of-Memory NMF on CPU/GPU Architectures
Authors: Boureima Ismael , Bhattarai Manish , Eren Maksim , Skau Erik , Romero Philip , Eidenbenz Stephan , Alexandrov Boian
Published: April 2023

We propose an efficient distributed out-of-memory implementation of the Non-negative Matrix Factorization (NMF) algorithm for heterogeneous high-performance-computing (HPC) systems. The proposed implementation is based on prior work on NMFk, which can perform automatic model selection and extract latent variables and patterns from data. In this work, we extend NMFk by adding support for dense and sparse matrix operation on multi-node, multi-GPU systems. The resulting algorithm is optimized for out-of-memory (OOM) problems where the memory required to factorize a given matrix is greater than the available GPU memory. Memory complexity is reduced by batching/tiling strategies, and sparse and dense matrix operations are significantly accelerated with GPU cores (or tensor cores when available). Input/Output (I/O) latency associated with batch copies between host and device is hidden using CUDA streams to overlap data transfers and compute asynchronously, and latency associated with collective communications (both intra-node and inter-node) is reduced using optimized NVIDIA Collective Communication Library (NCCL) based communicators. Benchmark results show significant improvement, from 32X to 76x speedup, with the new implementation using GPUs over the CPU-based NMFk. Good weak scaling was demonstrated on up to 4096 multi-GPU cluster nodes with approximately 25,000 GPUs when decomposing a dense 340 Terabyte-size matrix and an 11 Exabyte-size sparse matrix of density 10^{-6}.
Cancer- related protein profile of patient-derived and commercial glioblastoma cell lines exposed to Temozolomide
Authors: Wajdman Anna Maria Bielecka , Machnik Grzegorz , Linnebacher Michael , Linnebacher Christina , Obuchowicz Ewa
Published: April 2023

Purpose
Since recurrence is observed in almost all glioma patients deeper insight into mechanisms responsible for therapy resistance and identification of new biomarkers is urgently required.
In this study were analyzed differences in expression of 84 cancer- related proteins in three GBM cell lines: the commercial T98G cells and two patient-derived cell lines.
Materials and Methods
Influence of temozolomide (TMZ) on changes in proteins expression, cell morphology and migration was investigated. Analyzed lines were characterized by different remarkable plasticity of proteins expression and proteomic alterations induced by TMZ. Among 10 proteins expressed in all lines, 5 (Cathepsin b, FGF, Survivin, AXL, Osteopontin) were modulated by TMZ administration.
Results
As a result of TMZ exposition in both HROG02 and T98G cell lines proteins involved in chemoresistance and invasion (TIE-2, Thrombospondin) were detected. This suggests that TMZ promoted their malignant phenotype even further. In control culture (not subjected to TMZ) of HROG17 cells proteins involved in metabolism were strongly suppressed.
Conclusion
The presented data shed a new light on the immunometabolic profile of glioma proteome and its plasticity in response to Temozolomide interventions. Cathepsin b, FGF, Survivin, AXL and Osteopontin seem to be promising targets for a multimodal treatment that could be applied to inhibit GBM recurrence in the future.
Iron-oxide embemded lipid nanoparticles against ductal infiltration carcinoma as photodynamic therapy using red diode laser irradiation technique: Apoptosis , ferroptosis , EAC model
Authors: V sandhiya , K Senthilkumaran K , U Ubaidulla
Published: April 2023

The purpose of the current study is to examine the effectiveness of folic acid-tagged, PLGA/PEGylated iron oxide nanoparticles in overcoming multidrug resistance when used in PDT therapy. The raw medicine and produced nanoparticles were analysed using techniques like FTIR, DSC, XRD, TGA, and Raman spectra. The nanoparticles were produced using the emulsion solvent evaporation method, and they were examined using AFM, SEM, TEM, DLS, in-vitro release, in-vitro cytotoxicity analysis (MTT-assay), cell cycle analysis, in-vitro morphological alterations (PDT), in-vivo research, and other methods.The results of pre-formulation experiments showed that the drug and polymer are compatible, and the nano-formulation makes sure that the drug is well enclosed within the polymer matrix. According to the morphological examination, the nanoparticles had bilayer covering and were round and spherical in shape. Due to their capacity to incorporate into membranes and then translocate into cells where they affect a variety of cellular processes like mitochondrial respiration, ATP synthesis, and activity of drug efflux transporters, MTT-assay results show that PF68-loaded nanoparticles have a higher inhibition rate than other types. Cell cycle analysis research shows that when concentration increases, apoptosis initially increases, and necrosis then increases as concentration increases. According to an in-vitro photodynamic study, nanoparticles treated with lasers cause more cell death than nanoformulations do in a dose- and time-dependent manner. PDT therapy has greater anticancer activity than GANPs, according to an animal study. Conclusion: When compared to polyvinyl alcohol (PVA) loaded nanoparticles, the drug formulated as a nanocarrier with PF68 may have greater bioavailability and anticancer activity. PDT has been used as a co-therapy to ensure that tumour cells undergo morphological changes and to accelerate the rate of cell death. Finally, it was predicted that in the future, it would be a highly effective and well-thought-out treatment for breast cancer.
Exact analytical solution of the lateral spread of a particle beam in matter within a nonsmall angle limit
Authors: IKEGAMI Seiji
Published: April 2023

The authors have requested that this preprint be removed from Research Square.
New Variable Precision Reduction Algorithm for Decision Tables
Authors: Li Xu , Xiao Hongping , Tang Jianguo
Published: April 2023

Variable precision reduction (VPR) and positive region reduction (PRR) are common definitions in attribute reduction. The compacted decision table is an extension of a decision table. In this paper, we propose another extension, called the weighted decision table. In both types of decision tables, VPR is defined, and the corresponding discernibility matrices for the PRR are proposed. Then, algorithms for obtaining the PRR from the discernibility matrices are presented. In both types of decision tables, the relationship between VPR and PRR is established by comparing the corresponding discernibility matrices. If the precision of the VPR meets the given conditions, then the PRR algorithms can be used to obtain the results after modifying some decision values in the decision tables. An analysis of the modification process of the decision values and the compression process of decision tables is used to propose a new algorithm for VPR in decision tables that ensures credibility. The effectiveness of the proposed algorithm was evaluated by an experimental comparison with existing VPR algorithms.
Forecasting infection fatality rate of COVID-19, Measuring the effciency of several hybrid models.
Authors: SEBA Djillali
Published: April 2023

The main goal of this study is to study a significant index in epidemiology, the daily infection fatality rate in COVID-19. To do this, we put into application several hybrid models. We use machine learning tools such as Support Vector Machine, Relevance Vector Machine, Random Forest , and statistical models such as ARIMA, BATS and exponential smoothing. We measure the models’ performance using RMSE, MSE, and MAE
Train rescheduling with the consideration of re-servicing during a severe disruption
Authors: Zhao Junduo , Li Haiying , Meng Lingyun , Luan Xiaojie
Published: April 2023

Train dispatching is critical for the punctuality and reliability of passenger transportation, especially in railway systems with ticketing reservations required. In such a system, e.g., the high-speed railway in China, the service frequency is usually low on most lines, and passengers often have very limited route options (no diversity and flexibility). In the presence of disruptions, e.g., segment blockage, trains services may be canceled due to insufficient infrastructure capacity, which can cause big troubles to passengers since they are very unlikely to find alternative, consequently being stranded. Thus, transporting these stranded passengers becomes the most important but complicated task. In this study, we propose to add extra stops to the running trains, i.e., re-servicing, in order to effectively utilize the reduced capacity of tracks. We identify and formulate the complex relation between track utilization, trains stop adding, and passenger rerouting, based on a time-space network. Local rerouting of trains is represented, and the tracks in each station are categorized by main track (stopping with no passenger activities) and siding track (train passing without a stop is not allowed). An integer linear programming (ILP) model is constructed to solve the integrated problem of train rescheduling and passenger rerouting with the consideration of re-servicing, which includes train cancelation and extra stop adding. Finally, a Lagrangian solution framework is introduced to solve the NP-hard problem, and the method is test on a real-world network of Chinese high-speed railway, conducting several experiments to demonstrate the reasonability, effectiveness, and efficiency of the proposed method.
PORPHYRIA CUTANEATARDA: A CASE REPORT
Authors: Bakali E. El , Kerrouch H. , Chafi R. El , Hanafi T. , Zemmez Y. , Frikh R. , Hjira N.
Published: April 2023

Porphyria cutaneatarda (PCT) is a rare disease of porphyrin metabolism, related to a deficiency of Uroporphyrinogen decarboxylase activity. Clinically, it manifests itself by a skin fragility in photo-exposed areas. The characteristic biochemical profile of PCT, with elevated levels of urinary and plasma porphyrins, establishes the diagnosis. Treatment is based on phlebotomy, hydroxychloroquine (100 to 200 mg twice weekly) and control of susceptibility factors. We report a case of porphyria cutaneatarda in a male coast guard by profession.
STRATEGY FOR IMPLEMENTING THE FUNCTIONS OF VILLAGE CONSULTANCY AGENCY IN DISTRIBUTING COMMUNITY ASPIRATIONS
Authors:   Assistant Professor, Faculty of Law, Wisnuwardhana University., Soleh Mukhammad
Published: April 2023

The Village Consultative Body is an institution that carries out government functions whose members are representatives of the Village people based on regional representation and are determined democratically. The purpose of this research is to: First: Find a model accommodate and channel the aspirations of the village community. Second: Find the factors that hinder accommodating and channeling the aspirations of the village community. Third: Find a strategy to overcome the inhibiting factors in accommodating and channeling the aspirations of the village community. The research method used is empirical legal research with studies in Asrikaton Village, Pakis District, Malang Regency. The results of the study found that legally the implementation of the functions of the Village Consultative Body in accommodating and channeling the aspirations of the village community has been running according to regulations, but has not been optimal in carrying out the mandate of statutory regulations. The inhibiting factor is the lack of coordination and communication between the village government and the village consultative body, the human resources of the members of the village consultative body are still not professional. This strategy increases quality and professionalism as well as improves facilities and infrastructure.
A REVIEW OF CORRELATION AMONG THE ECONOMIC GROWTH, ECONOMIC UNCERTAINTY AND SUSTAINABLE DEVELOPMENT IN INDIA
Authors:   Assistant Professor, Department of Economics, BSNV PG College, Lucknow., Kumar Gupta Pawan
Published: April 2023

Sustainability of the natural resources is based on human efforts and the economic development is the base of the sustainable development of any country. But an economys development would increase when its economic uncertainty is at a low level. There are two objectives of the present research paper the first is to find the correlation among sustainable development, economic growth and economic uncertainty in India and the second is to access the impact of economic growth and economic uncertainty on the sustainable development of India. The present research is taking the period of 2000-2022 to access the correlation and impact of economic growth and economic uncertainty on sustainable development. The explorative, descriptive and correlation-regression methods are used to analyse the all shreds of evidence. The research statements are rejected with strong statistical evidences and both the objectives of the present research paper are achieved. As the economic uncertainty is reduced due to strong economic policies, the economic development takes place and makes the easy sustainable development path in India.
Academic management in war conditions: Chronicles of aggression and resistance experience of Ukrainian universities
Authors: Kozmenko Serhiy , Danko Yuriy , Kozlovskyi Serhii
Published: April 2023

The full-scale aggression unleashed by the russian invaders against Ukraine on February 24, 2022, became an unexpected horror that struck the life of this European country. The war radically changed absolutely everything and for everyone. It has crippled the fate of millions of innocent people, claimed the lives of tens of thousands.The crazy decisions of the russian leadership and the influx of its hordes have turned a huge number of Ukrainian cities and towns into hell. Destroyed or damaged urban infrastructure, homes and businesses, hospitals and schools.These monsters did not spare the universities either.
Distance learning as a tool for enhancing university academic management processes during the war
Authors: Kuzheliev Mykhailo , Zherlitsyn Dmytro , Nechyporenko Alina , Lutkovska Svitlana , Mazur Hennadii
Published: April 2023

The ongoing war in Ukraine has posed unprecedented challenges to traditional education systems, disrupting learning and affecting education quality. As universities adapt to these challenges, the growing reliance on distance learning strategies becomes crucial for maintaining academic management processes. This paper investigates the role of distance learning tools in addressing wartime challenges and enhancing university academic management.Utilizing a mixed-methods approach, the study combines quantitative data analysis of student performance with qualitative insights from educators and students affected by the war. The results prove the effectiveness of distance learning tools in maintaining education quality during the war while also addressing the unique challenges faced by universities in conflict areas.The findings reveal that distance learning tools serve as a valuable resource for universities to mitigate the negative impact of the war on education quality as part of academic management processes. However, specific challenges such as developing digital competencies, ensuring access to technology, and designing effective distance learning materials must be addressed in war-related disruptions.The quantitative analysis of student performance data highlights the potential of innovative distance learning tools in maintaining education quality during crises and wars. However, the efficiency of their use during the large-scale war in Ukraine has shown a decline and thus necessitates further research. Nevertheless, these insights provide valuable guidance for educators and academician managers to support students and educators during challenging times.
Taras Shevchenko National University of Kyiv: Navigating education as a frontline during times of war
Authors: Bugrov Volodymyr , Gozhyk Andrii , Starostina Alla , Bilovodska Olena , Kochkina Nataliia
Published: April 2023

This study aims to highlight the resilience and innovation of Taras Shevchenko National University of Kyiv (KNU) in the face of internal and external risks, particularly during times of war and political instability. KNU’s commitment to advancing knowledge is evident through its successful maintenance of educational, scientific, and economic activities and its proactive measures to counter propaganda and misinformation. The university has prioritized the psychological and emotional support of its students and staff and has minimized student losses by implementing flexible forms of education and individual counseling. KNU has also strengthened its international partnerships and grant initiatives. Despite significant losses, the university has maintained its material and technical infrastructure by leveraging various funding sources and developing a strategic trajectory toward an entrepreneurial model. KNU’s strategic objectives include transforming the university into the core of innovation clusters, establishing a risk management system, and monitoring the labor market. KNU’s experience could provide valuable insights for other universities facing similar challenges, emphasizing the importance of effective risk management, crisis communication, and psychological support for the academic community.
AcknowledgmentThe authors express their gratitude to the Armed Forces of Ukraine for their bravery and resilience in the fight for the country’s freedom and independence against new threats in the 21st century. They also thank the Taras Shevchenko National University of Kyiv staff for their unwavering commitment, selflessness, and psychological fortitude, enabling maintaining and expanding the university’s resources despite challenging circumstances. Overall, the authors are optimistic about the future and confident in the prospect of victory.
GESTÃO DE RESÍDUOS NOS LABORATÓRIOS DA UNIVERSIDADE DE ÉVORA (2007-2021)
Authors: Capelo Sofia , Siquenique Guilhermina , Morgado João
Published: April 2023

WASTE MANAGEMENT IN THE LABORATORIES OF THE UNIVERSITY OF ÉVORA (2007-2021). Waste generated in university laboratories is an important component of university waste management, especially regarding their hazardousness and, also, the cost involved in their forwarding. To make a good management of this waste, it is essential to know the amount generated and the composition. However, this type of information remains scarce. In this work, waste from the laboratories of the University of Évora (Portugal) was surveyed from 2007 to 2021, using existing records. In the 15 years under analysis, the laboratories of the University of Évora generated about 61 tonnes of waste, 88% of which were hazardous and 66% were in the liquid state. In addition, only 3% of this waste was subjected to recovery operations. Moreover, the COVID-19 pandemic contributed to a double increase in hospital waste generated and to a substantially decreased of hazardous liquid waste from the laboratory activity, especially in the first year of the pandemic. In 2021, the second year of the pandemic, there was a “return to normality”, reaching the highest value of waste generated in the laboratories, of 8.6 tonnes. Finally, possible solutions for improving laboratory waste management were also discussed.
Peran Kepala Madrasah dalam Pelaksanaan Gerakan Literasi Sekolah di MTsN 4 Kota Surabaya
Authors: Lista Nadiya Eka Fitriya Air , Ahwan Muh Ahlis
Published: April 2023

Penelitian ini bertujuan untuk mendeskripsikan peran kepala madrasah dalam Gerakan Literasi Sekolah (GLS) dan pelaksanaan GLS di MTsN 4 Kota Surabaya. Metode yang digunakan ialah kualitatif dengan pengumpulan data melalui observasi, wawancara, dan dokumentasi. Informan penelitian ini adalah kepala madrasah, kepala perpustakaan, guru Bahasa Indonesia, dan siswa di MTsN 4 Kota Surabaya. Analisis datanya menggunakan reduksi data, penyajian data, penarikan kesimpulan dan verifikasi. Hasil penelitian menunjukkan bahwa peran kepala MTsN 4 Kota Surabaya dalam gerakan literasi sekolah, antara lain sebagai educator, manager, leader, dan motivator. Pelaksanaan gerakan literasi sekolah di MTsN 4 Kota Surabaya dilakukan dengan tiga tahapan yaitu pembiasaan, pengembangan dan pembelajaran. Dengan adanya peran kepala sekolah dan terlaksananya GLS di MTsN 4 Kota Surabaya diharapkan pimpinan semakin menyadari akan hak dan kewajibannya sebagai figure yang mampu melaksanakan gerakan literasi di satuan pendidikan yang muaranya dapat menumbuhkan budi pekerti siswa, sehingga jangka panjangnya diharapkan dapat menghasilkan siswa yang memiliki kemampuan literasi tinggi.
Health risk assessment of trace elements in the Tonle Sap Great Lake and the Tonle Sap River in Cambodia during the rainy season
Authors: Phan Kongkea , Kim Kyoung-Woong
Published: April 2023

To investigate the potential health risk of trace elements in the Tonle Sap Great Lake system, lake (n = 37) and river (n = 14) water samples were collected and analyzed for 19 trace elements (Ag, Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Ga, Mn, Mo, Ni, Pb, Se, Tl, U and Zn) using inductively coupled plasma mass spectrometry. As a result, Cd was not detected in any river and lake water samples. Al, Fe and Mn in lake water exceeded the regulation limits of Cambodia, USEPA and WHO. Health risk assessment using the USEPA model indicated that male and female Cambodian residents are at minimal risk of non-carcinogenic effects from single and mixed trace elements through lake and river water consumption. Nevertheless, As, Tl, Co, Ba, Mn and Cr might pose high potential health risks to consumers which requires more attention. Therefore, regular monitoring and further studies are required to investigate the pollution trends and toxic behavior of these trace elements in the Tonle Sap Great Lake system.
Frequency and degradation of SARS-CoV-2 markers N1, N2, and E in sewage
Authors: Hart John J. , Jamison Megan N. , McNair James N. , Szlag David C.
Published: April 2023

Coronavirus disease 2019 (COVID-19) is an infectious disease that is mainly spread through aerosolized droplets containing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is excreted in feces by infected individuals. Sewage surveillance has been applied widely to obtain data on the prevalence of COVID-19 in whole communities. We used SARS-CoV-2 gene targets N1, N2, and E to determine the prevalence of COVID-19 at both municipal and building levels. Frequency analysis of wastewater testing indicated that single markers detected only 85% or less of samples that were detected as positive for SARS-CoV-2 with the three markers combined, indicating the necessity of pairing markers to lower the false-negative rate. The best pair of markers in both municipal and building level monitoring was N1 and N2, which correctly identified 98% of positive samples detected with the three markers combined. The degradation rates of all three targets were assessed at two different temperatures (25 and 35 °C) as a possible explanation for observed differences between markers in frequency. Results indicated that all three RNA targets degrade at nearly the same rate, indicating that differences in degradation rate are not responsible for the observed differences in marker frequency.
Dynamic population normalisation in wastewater-based epidemiology for improved understanding of the SARS-CoV-2 prevalence: a multi-site study
Authors: Sweetapple Chris , Wade Matthew J. , Melville-Shreeve Peter , Chen Albert S. , Lilley Chris , Irving Jessica , Grimsley Jasmine M.S. , Bunce Joshua T.
Published: April 2023

Wastewater-based epidemiology (WBE) is a valuable tool for monitoring the circulation of COVID-19. However, while variations in population size are recognised as major sources of uncertainty, wastewater SARS-CoV-2 measurements are not routinely population-normalised. This paper aims to determine whether dynamic population normalisation significantly alters SARS-CoV-2 dynamics observed through wastewater monitoring, and whether it is beneficial or necessary to provide an understanding of COVID-19 epidemiology. Data from 394 sites in England are used, and normalisation is implemented based on ammoniacal nitrogen and orthophosphate concentrations. Raw and normalised wastewater SARS-CoV-2 metrics are evaluated at the site and spatially aggregated levels are compared against indicators of prevalence based on the Coronavirus Infection Survey and Test and Trace polymerase chain reaction test results. Normalisation is shown, on average, to have a limited impact on overall temporal trends. However, significant variability in the degree to which it affects local-level trends is observed. This is not evident from previous WBE studies focused on single sites and, critically, demonstrates that while the impact of normalisation on SARS-CoV-2 trends is small on average, this may not always be the case. When averaged across many sites, normalisation strengthens the correlation between wastewater SARS-CoV-2 data and prevalence indicators; however, confidence in the improvement is low.
Investigation of water utility efficiency features: production variables and the DEA model choices
Authors: Gidion Dickson K.
Published: April 2023

Data envelopment analysis (DEA) has been used for decades to investigate utility efficiency- and management-specific features. DEA has been extended in various industries with different models that consume different types of production variables to meet industry-specific efficiencies. However, the discernment of production variables and proper DEA models is still a problem in the water industry. Ratio information has been used in DEA for absolute data to assess utility efficiency- and management-specific features; using ratios in DEA for absolute data produces wrong efficiency results because the convexity axiom of DEA for absolute data is not satisfied. This study presents a collection of information and an assessment of DEA for absolute and ratio data to identify the strengths of the production variables and the DEA model that are appropriate for assessing utility efficiency- and management-specific features. The analysis shows that the use of ratio variables in DEA for ratios improves utility economics, the working environment, and the social trust dimension.
Fitoplancton de la zona litoral del departamento del Atlántico, Colombia
Authors: De la Parra-Guerra Ana C. , García-Alzate Carlos , Gutiérrez-Moreno (†) Luis C.
Published: April 2023

Las comunidades biológicas están conformadas por grupos taxonómicos, donde el fitoplancton, como productor primario, tiene una relevancia ecológica: sirve para comprender la estructura y el funcionamiento de un ecosistema y la manera en la que los organismos interactúan con los parámetros fisicoquímicos. Con el fin de evaluar la riqueza y la abundancia del fitoplancton de la zona litoral del departamento del Atlántico y su asociación con las variables fisicoquímicas para conocer sus cambios espaciales y temporales, se realizaron monitoreos durante el año 2013. Se ubicaron cinco zonas a lo largo de la zona litoral como Astilleros, Santa Verónica, Puerto Velero, Puerto Colombia y Mallorquín. En cada una se establecieron tres estaciones, distribuidas en línea recta, con distancia entre ellas de 5 km. Para la recolecta de las muestras de fitoplancton y clorofila a (Cl-a), en cada una de las estaciones se filtraron 24 L de agua superficial, con un tambor cónico con malla de diámetro de poro de 24 µm y con botellas ámbar de 500 mL, respectivamente, las cuales se mantuvieron en oscuridad. Adicionalmente, se registraron in situ variables fisicoquímicas.  Se identificaron un total de 560   cél.mL-1 de fitoplancton, agrupadas en 47 especies y cinco clases. Las Bacillariophyceae presentaron las mayores densidad y riqueza, seguidas de las Dinophyceae, Cyanophyceae, Euglenophyceae y Chlorophyceae. Chaetoceros sp. (83 cél.mL-1) y Chaetoceros lorenzianus (64 cél.mL-1) fueron las especies más abundantes y más representativas, precisamente por su abundancia. El análisis de Jaccard mostró una disimilitud de un 80 % entre las estaciones 5A, 5B y 5C, lo que indica una composición diferente. En general, las estaciones de monitoreo se comportaron como sitios contaminados con alta influencia de las aguas del río Magdalena.
Diversidad de coleópteros acuáticos en la parte baja del Río Ancho, Dibulla, La Guajira, Colombia
Authors: Laguna Portela Carlos Farid , Granados-Martínez Cristian , Fuentes Reinés Juan M.
Published: April 2023

El presente estudio evaluó la diversidad de coleópteros acuáticos en un tramo de la parte baja del río Ancho en el municipio de Dibulla, La Guajira (Colombia). Los muestreos se realizaron entre agosto y noviembre del 2016 en distintos microhábitats (hojarasca, sedimento, macrófitas, piedras y raíces) con una red Surber de 250 μm de abertura de malla. También se midieron variables fisicoquímicas con el fin de determinar relaciones existentes con los individuos. Se recolectaron 621 coleópteros, distribuidos en seis familias y nueve géneros. Las familias más abundantes fueron Elmidae y Psephenidae. De los microhábitats muestreados, el sedimento reflejó la mayor abundancia. Se registraron por primera vez para la región los géneros Hydrocanthus (Dytiscidae), Hydrochus (Hydrochidae), Megadytes (Dytiscidae), Micrapate (Bostrichidae) y Psephenops (Psephenidae). En este estudio la familia Elmidae fue la que registró mayor diversidad de géneros, coincidiendo con lo hallado por otros autores. Las variables explicativas del análisis de correspondencia canónica (ACC) indican que existe preferencia de los géneros Heterelmis, Phanocerus, Psephenops y Macrelmis por los microhábitats macrófitas y raíces. Los resultados de este trabajo indican que el río Ancho constituye un importante reservorio de diversidad de coleópteros acuáticos para los ríos de la Sierra Nevada de Santa Marta (SNSM).
Tafonomía forense: estudio experimental del proceso de descomposición de un cuerpo sumergido en una ciénaga del Caribe colombiano
Authors: Toloza-Leones Anyely , Valverde-Castro César
Published: April 2023

En este trabajo se documenta el proceso de descomposición de un cadáver de cerdo en un ecosistema léntico, simulando la descomposición de un cuerpo humano, en la Ciénaga Inasika del municipio El Banco (Departamento del Magdalena) con el fin de estimar el intervalo postinmersión (ISPM). Para esto, se usó como biomodelo un cadáver de cerdo (Sus scrofa domesticus) sacrificado por contusión craneal; el cadáver fue enjaulado de tal manera que ingresaran los insectos, más no los grandes carroñeros. Posteriormente se sumergió en la ciénaga a 80 cm de profundidad con el fin de documentar los cambios post mortem y la sucesión entomológica. Se estableció un ISPM de 11 días y se documentaron seis fases de descomposición, en las cuales se recolectaron dípteros pertenecientes a las familias de Muscidae, Calliphoridae y Sarcophagidae. Las fases de descomposición observadasfueron: fresca, flotación temprana, descomposición flotante, deterioro de la hinchazón, restos flotantes y restos hundidos. Finalmente, dada la necesidad que tiene el país de expandir el conocimiento en ciencias forenses a causa del conflicto armado y la delincuencia común, buscamos desarrollar herramientas que faciliten los procesos legales, ayudando a esclarecer casos donde se tenga poca información sobre los hechos seguidos a la muerte de una persona.
Lentiviral Micro-dystrophin Gene Treatment into Late-stage mdx Mice
for Duchenne Muscular Dystrophy Disease
Authors: Eren Selen Abanuz , Tastan Cihan , Karadeniz Kevser Buse , Turan Raife Dilek , Cakirsoy Didem , Kancagi Derya Dilek , Yilmaz Sevdican Ustun , Oztatlici Mustafa , Oztatlici Hulya , Ozer Samed , Tumentemur Gamze , Baykal Ahmet Tarık , Ovali Ercument
Published: April 2023

Aim:

Duchenne Muscular Dystrophy (DMD) results in a deficiency of dystrophin expression
in patient muscle fibers, leading to progressive muscle degeneration. Treatment of DMD has undertaken
current transformation with the advancement of novel gene therapy and molecular biology
techniques, which are secure, well-tolerated, and effective therapeutic approaches.

Introduction:

DMD gene therapies have mainly focused on young DMD patients as in vivo animal
model trials have been performed in 0–1-month DMD mice. However, it has not yet been answered
how micro-dystrophin encoding lentiviral treatment affects Dystrophin expression and DMD symptoms
in 10-month mdx mice.

Methods:

We planned to integrate the micro-Dystrophin gene sequence into the muscle cells by viral
transfer, using micro-Dystrophin-encoding lentivirus to reduce the dystrophic pathology in late-stage
dmd mice. The histopathological and physiological-functional regeneration activities of the lentiviralmicro-
Dystrophin gene therapy methods were compared, along with changes in temporal Dystrophin
expression and their functionality, toxicity, and gene expression level.

Results:

Here, we showed that the micro-dystrophin transgene transfers intramuscularly and intraperitoneally
in late-stage dmd-mdx-4cv mice restored dystrophin expression in the skeletal and cardiac
muscle (p <0.001). Furthermore, motor performance analysis, including hanging and tracking tests,
improved statistically significantly after the treatment (p <0.05).

Conclusion:

Consequently, this study suggests that patients in the late stages of muscular dystrophy
can benefit from lentiviral micro-dystrophin gene therapies to present an improvement in dystrophic
muscle pathology.
Molecular Modeling of some 1,3,4-Oxadiazole Derivatives as EGFR Inhibitors for the Treatment of Cancer
Authors: Patil Shital M. , Bhandari Shashikant V. , Patil Varsha A. , Randive Vrushali , Mahadik Indrani
Published: April 2023

Background:

Cancer is a group of illnesses characterised by the impartial increase and unfolding
of somatic cells. A variety of natural compounds, such as curcumin, zingiberine and their composites,
and synthetic organic derivatives of imidazole, benzothiazole, oxadiazole, quinazoline etc., have been
developed as anticancer agents. But many of these show various side effects, drug resilience, and toxicity
to the normal cells of the host body. Therefore, there is still demand to develop new synthetic derivatives
to improve their pharmacological profile.

Objective:

The Aim of this study is to understand the binding mode and to check the drug likeliness of
numerous 1,3,4-oxadiazole derivatives as EGFR inhibitors for the treatment of cancer. The objective of
the study is to screen newly designed derivatives of 1,3,4-oxadiazole using molecular docking and
ADMET studies as EGFR inhibitors.

Methods:

In silico docking studies were performed using AutoDockVina software, and compounds were
further studied for ADME and toxicity using SwissADME and pkCSM software, respectively.

Results:

Considering the docking results, pharmacokinetic behaviour and toxicity profile, eight derivatives
(derivatives 2,3,4,5,10,13,16, and 17) showed potential as EGFR inhibitors.

Conclusion:

Compounds 2 and 3 showed the highest binding affinity in the pocket of EGFR and also
displayed a better pharmacokinetic profile. Therefore, these derivatives can be used in the management of
cancer and can be taken further for wet-lab studies.