bluuebunny/crossref_metadata_2025 huggingface dataset:
A tomographic approach to assessing the possibility of ring shake presence in standing chestnut trees
Authors: Proto Andrea R. , Cataldo Maria F. , Costa Corrado , Papandrea Salvatore F. , Zimbalatti Giuseppe
Published: September 2020

Ring shake is a widespread phenomenon affecting a great number of species of both softwood and hardwood and is found in trees grown in temperate and tropical climates. Chestnut (Castanea sativaMill.) represents one of the most important hardwood timbers that is very often affected by ring shake. This defect seems to be the only real limit to the spread and use of chestnut wood worldwide on a scale closer to the availability of this wood. The aim of this study was to examine the potential of tomographic measurement as a non-destructive method for predicting the possibility of the presence of ring shake in standing chestnut trees. For this reason, the experiments were carried out in a chestnut coppice stand where one hundred chestnut standards were monitored using an acoustic tomographic device, and subsequently harvested by a local company and cross-sectioned corresponding to the acoustic tests. This work proposed an applied approach to predicting and determining wood quality (sound wood vs. defective wood) from tomographic data. The model, based on a non-linear approach, showed that sonic tomography can identify ring shake in a tree trunk without affecting its biological activity, overcoming the difficulties of predicting ring shake using only visual inspection.
A Hitchhiker’s guide to the potato wart disease resistance galaxy
Authors: Prodhomme Charlotte , van Arkel Gert , Plich Jarosław , Tammes Jasper E. , Rijk Johan , van Eck Herman J. , Visser Richard G. F. , Vossen Jack H.
Published: September 2020

Key message

Two novel major effect loci (Sen4 and Sen5) and several minor effect QTLs for potato wart disease resistance have been mapped. The importance of minor effect loci to bring full resistance to wart disease was investigated. Using the newly identified and known wart disease resistances, a panel of potato breeding germplasm and Solanum wild species was screened. This provided a state-of-the-art “hitch-hikers-guide” of complementary wart disease resistance sources.

Potato wart disease, caused by the obligate biotrophic soil-born fungus Synchytrium endobioticum, is the most important quarantine disease of potato. Because of its huge impact on yield, the lack of chemical control and the formation of resting spores with long viability, breeding for resistant varieties combined with strict quarantine measures are the only way to efficiently and durably manage the disease. In this study, we set out to make an inventory of the different resistance sources. Using a Genome-Wide Association Study (GWAS) in the potato breeding genepool, we identified Sen4, associated with pathotypes 2, 6 and 18 resistance. Associated SNPs mapped to the south arm of chromosome 12 and were validated to be linked to resistance in one full-sib population. Also, a bulked segregant analysis combined with a Comparative Subsequence Sets Analysis (CoSSA) resulted in the identification of Sen5, associated with pathotypes 2, 6 and 18 resistance, on the south arm of chromosome 5. In addition to these two major effect loci, the GWAS and CoSSA allowed the identification of several quantitative trait loci necessary to bring full resistance to certain pathotypes. Panels of varieties and Solanum accessions were screened for the presence of Sen1, Sen2, Sen3, Sen4 and Sen5. Combined with pedigree analysis, we could trace back some of these genes to the ancestral resistance donors. This analysis revealed complementary resistance sources and allows elimination of redundancy in wart resistance breeding programs.
Bone marrow stimulation for talar osteochondral lesions at long-term follow-up shows a high sports participation though a decrease in clinical outcomes over time
Authors: Lambers Kaj T. A. , Dahmen Jari , Altink J. Nienke , Reilingh Mikel L. , van Bergen Christiaan J. A. , Kerkhoffs Gino M. M. J.
Published: September 2020

Purpose

Although bone marrow stimulation (BMS) as a treatment for osteochondral lesions of the talus (OCLT) shows high rates of sport resumption at short-term follow-up, it is unclear whether the sports activity is still possible at longer follow-up. The purpose of this study was, therefore, to evaluate sports activity after arthroscopic BMS at long-term follow-up.

Methods

Sixty patients included in a previously published randomized-controlled trial were analyzed in the present study. All patients had undergone arthroscopic debridement and BMS for OCLT. Return to sports, level, and type were assessed in the first year post-operative and at final follow-up. Secondary outcome measures were assessed by standardized questionnaires with use of numeric rating scales for pain and satisfaction and the Foot and Ankle Outcome Score (FAOS).

Results

The mean follow-up was 6.4 years (SD ± 1.1 years). The mean level of activity measured with the AAS was 6.2 pre-injury and 3.4 post-injury. It increased to 5.2 at 1 year after surgery and was 5.8 at final follow-up. At final follow-up, 54 patients (90%) participated in 16 different sports. Thirty-three patients (53%) indicated they returned to play sport at their pre-injury level. Twenty patients (33%) were not able to obtain their pre-injury level of sport because of ankle problems and eight other patients (13%) because of other reasons. Mean NRS for pain during rest was 2.7 pre-operative, 1.1 at 1 year, and 1.0 at final follow-up. Mean NRS during activity changed from 7.9 to 3.7 to 4.4, respectively. The FAOS scores improved at 1 year follow-up, but all subscores significantly decreased at final follow-up.

Conclusion

At long-term follow-up (mean 6.4 years) after BMS for OCLT, 90% of patients still participate in sports activities, of whom 53% at pre-injury level. The AAS of the patients participating in sports remains similar pre-injury and post-operatively at final follow-up. A decrease over time in clinical outcomes was, however, seen when the follow-up scores at 1 year post-operatively were compared with the final follow-up.

Level of evidence

Level II.
Higher treatment effect after total knee arthroplasty is associated with higher patient satisfaction
Authors: Lützner Jörg , Beyer Franziska , Günther Klaus-Peter , Huber Jörg
Published: September 2020

Purpose

The aim of this study was to investigate what influence the treatment effect after total knee arthroplasty (TKA) had on patient satisfaction.

Methods

Prospective registry data of a University-based arthroplasty centre were used. 582 patients with unilateral bicondylar TKA were analyzed. Treatment effect (TE) was deduced from Oxford Knee Score (OKS) before and one year after surgery. Positive values correspond to improved symptoms (maximum 1.0 reflect no symptoms at all) and negative values correspond to deterioration of symptoms. Satisfaction on a visual-analogue scale from 0 to 10 and the willingness to undergo TKA surgery again was assessed one year after surgery.

Results

The mean OKS improved from 22.1 before to 36.7 one year after TKA. Treatment effects ranged from 1.0 to –0.62 with a mean TE of 0.56. Taking an individual treatment effect of 0.2 as a cut-off between responder and non-responder, a total of 85.8% would be classified as responder after TKA. The mean satisfaction score with the TKA was 8.1. There was a significant correlation between the individual treatment effect and satisfaction after TKA (p < 0.001). The majority of patients (84.5%) would undergo surgery again. Patients not willing to undergo surgery again or those uncertain about this had lower satisfaction scores, a lower treatment effect and were more often female compared to patients who would undergo surgery again.

Conclusion

Higher individual treatment effects resulted in higher patient satisfaction and willingness to undergo surgery again. However, some patients with a relatively low treatment effect were highly satisfied, which indicates the need for both information.

Level of evidence

II.
In situ monitoring of photocatalyzed isomerization reactions on a microchip flow reactor by IR-MALDI ion mobility spectrometry
Authors: Prüfert Chris , Urban Raphael David , Fischer Tillmann Georg , Villatoro José , Riebe Daniel , Beitz Toralf , Belder Detlev , Zeitler Kirsten , Löhmannsröben Hans-Gerd
Published: September 2020

The visible-light photocatalytic E/Z isomerization of olefins can be mediated by a wide spectrum of triplet sensitizers (photocatalysts). However, the search for the most efficient photocatalysts through screenings in photo batch reactors is material and time consuming. Capillary and microchip flow reactors can accelerate this screening process. Combined with a fast analytical technique for isomer differentiation, these reactors can enable high-throughput analyses. Ion mobility (IM) spectrometry is a cost-effective technique that allows simple isomer separation and detection on the millisecond timescale. This work introduces a hyphenation method consisting of a microchip reactor and an infrared matrix-assisted laser desorption ionization (IR-MALDI) ion mobility spectrometer that has the potential for high-throughput analysis. The photocatalyzed E/Z isomerization of ethyl-3-(pyridine-3-yl)but-2-enoate (E-1) as a model substrate was chosen to demonstrate the capability of this device. Classic organic triplet sensitizers as well as Ru-, Ir-, and Cu-based complexes were tested as catalysts. The ionization efficiency of the Z-isomer is much higher at atmospheric pressure which is due to a higher proton affinity. In order to suppress proton transfer reactions by limiting the number of collisions, an IM spectrometer working at reduced pressure (max. 100 mbar) was employed. This design reduced charge transfer reactions and allowed the quantitative determination of the reaction yield in real time. Among 14 catalysts tested, four catalysts could be determined as efficient sensitizers for the E/Z isomerization of ethyl cinnamate derivative E-1. Conversion rates of up to 80% were achieved in irradiation time sequences of 10 up to 180 s. With respect to current studies found in the literature, this reduces the acquisition times from several hours to only a few minutes per scan.
Gadoxetic acid-enhanced MRI in primary sclerosing cholangitis: added value in assessing liver function and monitoring disease progression
Authors: Elkilany Aboelyazid , Geisel Dominik , Müller Tobias , Fischer Andreas , Denecke Timm
Published: September 2020

Purpose

To investigate the added value of gadoxetic acid-enhanced MRI in monitoring liver function and disease progression in patients with primary sclerosing cholangitis (PSC).

Methods

We retrospectively identified 104 consecutive patients (75 males; mean age 41.98 ± 12.5 years) with confirmed diagnosis of PSC who underwent 227 gadoxetic acid-enhanced MRI examinations between January 2008 and May 2019. Relative enhancement (RE) of the liver was correlated with the results of liver function tests (LFTs), scoring models (Model for End-Stage Liver Disease (MELD) score, Mayo Risk Score (MRS), Amsterdam-Oxford model (AOM)), and qualitative MRI findings. In addition, results were analyzed separately for excretory MRI examinations (n = 164) and nonexcretory examinations (n = 63) depending on excretion of gadoxetic acid into the common bile duct in the hepatobiliary phase (HBP).

Results

There was a significant correlation of RE with MRS (r = − 0.652), MELD score (r = − 0.474), AOM (r = − 0.468), and LFTs (P < 0.001). RE and albumin were significantly higher in the excretory group whereas scoring models, bilirubin, aspartate aminotransferase, alkaline phosphatase, and international normalized ratio were lower (P < 0.001). RE was lower in segments with absent HBP gadoxetic acid excretion into dilated bile ducts, reduced HBP parenchymal enhancement, atrophy, T2 hyperintensity, and bile duct abnormalities (P < 0.001).

Conclusion

Relative enhancement of the liver in gadoxetic acid-enhanced MRI can be used to evaluate global and regional liver function and monitor disease progression in patients with PSC. Hepatobiliary phase gadoxetic acid biliary excretion appears to be a reproducible qualitative parameter for evaluating disease severity that can be easily integrated into routine clinical practice.
Colorectal cancer in Crohn’s colitis is associated with advanced tumor invasion and a poorer survival compared with ulcerative colitis: a retrospective dual-center study
Authors: Vetter Leonie E. , Merkel Susanne , Bénard Alan , Krautz Christian , Brunner Maximilian , Mittelstädt Anke , Schlegel Nicolas , Wiegering Armin , Germer Christoph-Thomas , Weber Klaus , Grützmann Robert , Weber Georg F.
Published: September 2020

Purpose

Colorectal cancer is a well-recognized complication of inflammatory bowel diseases (IBD), such as ulcerative colitis (UC) and Crohn’s colitis (CC). In this study, we assess the clinico-pathological features and outcomes of patients with colorectal cancer from UC in comparison with CC.

Methods

Data of all patients with colitis-associated cancer (CAC) who underwent surgery at Erlangen or Würzburg University Clinic between 1995 and 2015 were selected. Clinical, histopathological, and survival data were analyzed retrospectively.

Results

Of all 88 patients with CAC, 20 patients had Crohn’s colitis and 68 patients had ulcerative colitis. We observed a young median age at tumor diagnosis (49.5 years UC; 45.5 years CC, p = 0.208) in both diseases and a long median disease duration before CAC (19 years UC; 18 years CC; p = 0.840). Patients with CC suffered more often from rectal cancer (14 (70.0%) in CC; 23 (33.8%) in UC; p = 0.005) and advanced tumor stages (8 (47.0%) pT4 in CC; 14 (25.0%) pT4/ypT4 in UC; p = 0.008). Five-year overall survival rate was 39.3% for CC and 67.1% for UC (p = 0.009 for difference between the groups). Survival did not differ significantly between UC and CC in the multivariate analysis after correction for UICC tumor stage.

Conclusion

CAC in CC showed advanced tumor stages associated with reduced survival compared with CAC in UC. This may be explained by less intense surveillance in patients with CC leading to delayed cancer diagnosis.
Intra-individual physiological response of recreational runners to different training mesocycles: a randomized cross-over study
Authors: Düking Peter , Holmberg Hans-Christer , Kunz Philipp , Leppich Robert , Sperlich Billy
Published: September 2020

Purpose

Pronounced differences in individual physiological adaptation may occur following various training mesocycles in runners. Here we aimed to assess the individual changes in performance and physiological adaptation of recreational runners performing mesocycles with different intensity, duration and frequency.

Methods

Employing a randomized cross-over design, the intra-individual physiological responses [i.e., peak ($${\dot{\text V}}{\text O}_{2 {\rm peak}}$$V˙O2peak) and submaximal ($${\dot{\text V}}{\text O}_{2 {\rm submax}}$$V˙O2submax) oxygen uptake, velocity at lactate thresholds(V2, V4)] and performance (time-to-exhaustion (TTE)) of 13 recreational runners who performed three 3-week sessions of high-intensity interval training (HIIT), high-volume low-intensity training (HVLIT) or more but shorter sessions of HVLIT (high-frequency training; HFT) were assessed.

Results

$${\dot{\text V}}{\text O}_{2 {\rm submax}}$$V˙O2submax, V2,V4and TTE were not altered by HIIT, HVLIT or HFT (p > 0.05).$${\dot{\text V}}{\text O}_{2 {\rm peak}}$$V˙O2peakimproved to the same extent following HVLIT (p = 0.045) and HFT (p = 0.02). The number of moderately negative responders was higher following HIIT (15.4%); and HFT (15.4%) than HVLIT (7.6%). The number of very positive responders was higher following HVLIT (38.5%) than HFT (23%) or HIIT (7.7%). 46% of the runners responded positively to two mesocycles, while 23% did not respond to any.

Conclusion

On a group level, none of the interventions altered$${\dot{\text V}}{\text O}_{2 {\rm submax}}$$V˙O2submax, V2, V4or TTE, while HVLIT and HFT improved$${\dot{\text V}}{\text O}_{2 {\rm peak}}$$V˙O2peak. The mean adaptation index indicated similar numbers of positive, negative and non-responders to HIIT, HVLIT and HFT, but more very positive responders to HVLIT than HFT or HIIT. 46% responded positively to two mesocycles, while 23% did not respond to any. These findings indicate that the magnitude of responses to HIIT, HVLIT and HFT is highly individual and no pattern was apparent.
The provision of nutritional advice and care for cancer patients: a UK national survey of healthcare professionals
Authors: Murphy Jane L. , Munir Fehmidah , Davey Fiona , Miller Laura , Cutress Ramsey , White Rhys , Lloyd Megan , Roe Justin , Granger Carol , Burden Sorrel , Turner Lesley
Published: September 2020

Purpose

People living with and beyond cancer often experience nutrition-related issues and should receive appropriate advice on nutrition that is consistent and evidence based. The aim of this study was to investigate current practice for the provision of nutritional care by healthcare professionals (HCPs) from a UK national survey produced by the National Institute for Health Research (NIHR) Cancer and Nutrition Collaboration.

Methods

An online survey sent to professional groups and networks included questions on discussing nutrition, providing information, awareness of guidelines, confidence in providing nutritional advice, training and strategies for improving nutritional management.

Results

There were 610 HCPs who responded including nurses (31%), dietitians (25%), doctors (31%) and speech and language therapists (9%). The majority of HCPs discusses nutrition (94%) and provides information on nutrition (77%). However, only 39% of HCPs reported being aware of nutritional guidelines, and just 20% were completely confident in providing nutritional advice. Awareness of guidelines varied between the different professional groups with most but not all dietitians reporting the greatest awareness of guidelines and GPs the least (p = 0.001). Those HCPs with a greater awareness of guidelines had received training (p = 0.001) and were more likely to report complete confidence in providing nutritional advice (p = 0.001).

Conclusion

Whilst HCPs discuss nutrition with cancer patients and may provide information, many lack an awareness of guidelines and confidence in providing nutritional advice. To ensure consistency of practice and improvements in patient care, there is scope for enhancing the provision of appropriate nutrition education and training.
The silent transition from curative to palliative treatment: a qualitative study about cancer patients’ perceptions of end-of-life discussions with oncologists
Authors: Kitta A. , Hagin A. , Unseld M. , Adamidis F. , Diendorfer T. , Masel E. K. , Kirchheiner K.
Published: September 2020

Objective

The aims of the study were to examine patients’ experiences of end-of-life (EOL) discussions and to shed light on patients’ perceptions of the transition from curative to palliative care.

Methods

This study was based on a qualitative methodology; we conducted semi-structured interviews with advanced cancer patients admitted to the palliative care unit (PCU) of the Medical University of Vienna. Interviews were recorded digitally and transcribed verbatim. Data were analyzed based on thematic analysis, using the MAXQDA software.

Results

Twelve interviews were conducted with patients living with terminal cancer who were no longer under curative treatment. The findings revealed three themes: (1) that the medical EOL conversation contributed to the transition process from curative to palliative care, (2) that patients’ information preferences were ambivalent and modulated by defense mechanisms, and (3) that the realization and integration of medical EOL conversations into the individual’s personal frame of reference is a process that needs effort and information from different sources coming together.

Conclusions

The results of the present study offer insight into how patients experienced their transition from curative to palliative care and into how EOL discussions are only one element within the disease trajectory. Many patients struggle with their situations. Therefore, more emphasis should be put on repeated offers to have EOL conversations and on early integration of aspects of palliative care into the overall treatment.
Can Heating Induce Borehole Closure?
Authors: Xie Xiyang , Bauer Andreas , Stenebråten Jørn F. , Bakheim Sigurd , Lavrov Alexandre , Fjær Erling , Kristiansen Tron G.
Published: September 2020

The current study shows that heating a cased borehole in low-permeability shale rock can induce plastic deformation, leading to the closure of the casing annulus and decreasing annulus connectivity. The thermally induced borehole closure is interesting for the field operation of plug and abandonment (P&A), as it potentially saves operation cost and time by avoiding cutting casing and cementing. Lab experiments and numerical simulations are implemented to investigate the thermally induced borehole closure. Pierre shale and a field shale are tested. The lab experiments are performed by heating the borehole wall in a 10-cm-OD hollow cylinder specimen. Here, a novel experimental setup is applied, allowing for measuring temperature and pore pressure at different radii inside the specimen. Both the experimental data and the post-test CT images of the rock samples indicate the rock failure by borehole heating, and under certain conditions, heating results in an annulus closure. The decrease of hydraulic conductivity through the casing annulus is observed, but this decrease is not enough to form the hydraulic-sealed annulus barrier, based on the results obtained so far. Lab-scale finite-element simulations aim to match the lab results to obtain poro-elastoplastic parameters. Then the field-scale simulations assess the formation of shale barriers by heating in field scenarios. Overall, (i) the lab experiments show that heating a borehole can increase the pore pressure in shale and hence induce rock failure; (ii) the numerical simulations match the experimental results reasonably well and indicate that the heating-induced borehole closure can sufficiently seal the casing annulus in the field-scale simulation.
A Spherical Indentation Study on the Mechanical Response of Selected Rocks in the Range from Very Hard to Soft with Particular Interest to Drilling Application
Authors: Saadati M. , Weddfelt K. , Larsson P.-L.
Published: September 2020

The focus of this work is toward an investigation of the mechanical response of a variety of rocks to indentation loading as a close condition to drilling application. A rock classification method is introduced based on the mechanical response of the rock when loaded by a spherical indenter. Spherical shape is selected for the indenter as a common geometry, which to some extent represents most of the new or worn inserts in drill bits. Both of the force–penetration and fragmentation responses are studied and the results are categorized accordingly. Indentation loading of a quasi-brittle medium like rock contains a complex three-dimensional stress state, in which the compressive strength, tensile strength, compaction behavior and the brittleness of the rock all together are reflected in its mechanical response. Therefore, depending on the type of the rock and its properties, this response is also very diverse for different rocks. Eight types of rocks are investigated and the results from the force–penetration and fragmentation responses are summarized into three different classes.
Get secure soon: attachment in abused adolescents and young adults before and after trauma-focused cognitive processing therapy
Authors: Rimane Eline , Steil Regina , Renneberg Babette , Rosner Rita
Published: September 2020

Severe posttraumatic stress symptoms (PTSS) are connected to a variety of health-related and interpersonal problems, among them are the insecure attachment orientations. However, psychotherapy seems to improve not only PTSS but also attachment insecurities. In a large multicenter, randomized clinical trial, the attachment characteristics and PTSS of 85 adolescents and young adults (aged 14–21 years) with clinically relevant abuse-related PTSS were assessed at study entry, at the end of treatment, and 3 months after the end of treatment. Participants were randomized either to a developmentally adapted cognitive processing therapy (D-CPT) or to a wait-list with treatment advice (WL/TA). The purpose of the study was to analyze the association between PTSS and attachment at study entry as well as changes in attachment during the trial. We found that attachment-related avoidance (AR avoidance) was positively associated with PTSS from both self-reports and clinician ratings, whereas attachment-related anxiety (AR anxiety) was only related to self-reported PTSS (Pearson correlation coefficients between 0.37 and 0.46). Changes in AR anxiety occurred in both conditions at some point during the study (baseline to 3-month follow-up effect size wasd = 0.60 for D-CPT andd = 0.44 for WL/TA) whereas for AR avoidance, only participants in D-CPT improved significantly (baseline to 3-month follow-up effect size wasd = 0.75). The results indicate that PTSS and attachment are connected. Positive changes in attachment insecurities brought about by trauma-focused psychotherapy seem possible.

Trial registration: German Clinical Trials Register (DRKS); Germanctr.de; identifier: DRKS00004787; date of registration: 18 March 2013.
A new three-step hybrid approach is a safe procedure for incisional hernia: early experiences with a single centre retrospective cohort
Authors: van den Dop L. Matthijs , de Smet Gijs H. J. , Bus Michaël P. A. , Lange Johan F. , Koch Sascha M. P. , Hueting Willem E.
Published: September 2020

Purpose

In this study, a three-step novel surgical technique was developed for incisional hernia, in which a laparoscopic procedure with a mini-laparotomy is combined: so-called ‘three-step incisional hybrid repair’. The aim of this study was to reduce the risk of intestinal lacerations during adhesiolysis and recurrence rate by better symmetrical overlap placement of the mesh.

Objectives

To evaluate first perioperative outcomes with this technique.

Methods

From 2016 to 2020, 70 patients (65.7% females) with an incisional hernia of > 2 and ≤ 10 cm underwent a elective three-step incisional hybrid repair in two non-academic hospitals performed by two surgeons specialised in abdominal wall surgery. Intra- and postoperative complications, operation time, hospitalisation time and hernia recurrence were assessed.

Results

Mean operation time was 100 min. Mean hernia size was 4.8 cm; 45 patients (64.3%) had a hernia of 1–5 cm, 25 patients (35.7%) of 6–10 cm. Eight patients had a grade 1 complication (11.4%), five patients a grade 2 (7.1%), two patients (2.8%) a grade 4 complication and one patient (1.4%) a grade 5 complication. Five patients had an intraoperative complication (7.0%), two enterotomies, one serosa injury, one omentum bleeding and one laceration of an epigastric vessel. Mean length of stay was 3.3 days. Four patients (5.6%) developed a hernia recurrence during a mean follow-up of 19.5 weeks.

Conclusion

A three-step hybrid incisional hernia repair is a safe alternative for incisional hernia repair. Intraoperative complications rate was low.
Epigenetic regulation of the lineage specificity of primary human dermal lymphatic and blood vascular endothelial cells
Authors: Tacconi Carlotta , He Yuliang , Ducoli Luca , Detmar Michael
Published: September 2020

Lymphatic and blood vascular endothelial cells (ECs) share several molecular and developmental features. However, these two cell types possess distinct phenotypic signatures, reflecting their different biological functions. Despite significant advances in elucidating how the specification of lymphatic and blood vascular ECs is regulated at the transcriptional level during development, the key molecular mechanisms governing their lineage identity under physiological or pathological conditions remain poorly understood. To explore the epigenomic signatures in the maintenance of EC lineage specificity, we compared the transcriptomic landscapes, histone composition (H3K4me3 and H3K27me3) and DNA methylomes of cultured matched human primary dermal lymphatic and blood vascular ECs. Our findings reveal that blood vascular lineage genes manifest a more ‘repressed’ histone composition in lymphatic ECs, whereas DNA methylation at promoters is less linked to the differential transcriptomes of lymphatic versus blood vascular ECs. Meta-analyses identified two transcriptional regulators, BCL6 and MEF2C, which potentially govern endothelial lineage specificity. Notably, the blood vascular endothelial lineage markers CD34, ESAM and FLT1 and the lymphatic endothelial lineage markers PROX1, PDPN and FLT4 exhibited highly differential epigenetic profiles and responded in distinct manners to epigenetic drug treatments. The perturbation of histone and DNA methylation selectively promoted the expression of blood vascular endothelial markers in lymphatic endothelial cells, but not vice versa. Overall, our study reveals that the fine regulation of lymphatic and blood vascular endothelial transcriptomes is maintained via several epigenetic mechanisms, which are crucial to the maintenance of endothelial cell identity.
Shear-horizontal waves in periodic layered nanostructure with both nonlocal and interface effects
Authors: Tian Ru , Liu Jinxi , Pan E. N. , Wang Yuesheng
Published: September 2020

The propagation of shear-horizontal (SH) waves in the periodic layered nanocomposite is investigated by using both the nonlocal integral model and the nonlocal differential model with the interface effect. Based on the transfer matrix method and the Bloch theory, the band structures for SH waves with both vertical and oblique incidences to the structure are obtained. It is found that by choosing appropriate interface parameters, the dispersion curves predicted by the nonlocal differential model with the interface effect can be tuned to be the same as those based on the nonlocal integral model. Thus, by propagating the SH waves vertically and obliquely to the periodic layered nanostructure, we could invert, respectively, the interface mass density and the interface shear modulus, by matching the dispersion curves. Examples are further shown on how to determine the interface mass density and the interface shear modulus in theory.
COVID-19 detection and disease progression visualization: Deep learning on chest X-rays for classification and coarse localization
Authors: Zebin Tahmina , Rezvy Shahadate
Published: September 2020

Chest X-rays are playing an important role in the testing and diagnosis of COVID-19 disease in the recent pandemic. However, due to the limited amount of labelled medical images, automated classification of these images for positive and negative cases remains the biggest challenge in their reliable use in diagnosis and disease progression. We implemented a transfer learning pipeline for classifying COVID-19 chest X-ray images from two publicly available chest X-ray datasets1,2. The classifier effectively distinguishes inflammation in lungs due to COVID-19 and Pneumonia from the ones with no infection (normal). We have used multiple pre-trained convolutional backbones as the feature extractor and achieved an overall detection accuracy of 90%, 94.3%, and 96.8% for the VGG16, ResNet50, and EfficientNetB0 backbones respectively. Additionally, we trained a generative adversarial framework (a CycleGAN) to generate and augment the minority COVID-19 class in our approach. For visual explanations and interpretation purposes, we implemented a gradient class activation mapping technique to highlight the regions of the input image that are important for predictions. Additionally, these visualizations can be used to monitor the affected lung regions during disease progression and severity stages.
Improving nutrient and water use efficiencies in multi-loop aquaponics systems
Authors: Goddek Simon , Keesman Karel J.
Published: September 2020

The sustainable development of agricultural systems where nutrients and water are recycled to a high degree is of enormous importance. Traditional aquaponics, where fish and plants are cultivated in one recirculating system, addresses these ecological challenges, but still struggles with its economical feasibility. Decoupled multi-loop aquaponics systems, in which the aquaculture and hydroponics subsystems are running autonomously, proved that they can keep up with the productivity of state-of-the-art hydroponics systems or even outscore them. Yet, a problem of such decoupled aquaponics systems was that plants require a high nutrient concentration, whereas fish prefer rather a clean water. In practice, the opposite is happening as the nutrients are added to the aquaculture units through the feed. This paper optimizes a recent approach showing that desalination technologies, such as reverse osmosis, can play an important role in reversing the concentrations within such systems without killing beneficial plant growth–promoting rhizobacteria thermally. The proposed integrated systems approach has the potential to make both periodical nutrient and water discharges and excessive fertilizer supplementation obsolete that would otherwise be necessary to maintain good water quality for the fish and an optimal nutrient solution for the plants.
Validity of the Draw a Person: A Quantitative Scoring System (DAP:QSS) for Clinically Evaluating Intelligence
Authors: Troncone Alda , Chianese Antonietta , Di Leva Alfonso , Grasso Maddalena , Cascella Crescenzo
Published: September 2020

To assess the psychometric properties of the Draw a Person: A Quantitative Scoring System (DAP:QSS), in 2543 children (M = 11.43 ± 3.06 years), correlations between drawings scores and Raven’s Matrices scores, age, and academic achievement were examined. Although older children (> 11 years) obtained higher drawing scores than younger ones (p < 0.001), age significantly correlated with DAP:QSS scores only in children younger than 11 years (r = 0.493, p < 0.001), indicating conflictive evidence for construct validity and a possible ceiling effect. No correlations emerged between DAP:QSS scores and grades (r = 0.056, p = 0.097). DAP:QSS scores were significantly associated with Raven’s Matrices score, but low correlation coefficients (0.156–0.498), low sensitivity (0.12), and high false negative (87.9%) and positive (82%) rates suggest poor DAP:QSS validity as an intelligence measure. The researchers concluded that DAP:QSS failed to produce a psychometrically sound assessment of children’s intellectual functioning.
Nintedanib and a bi-specific anti-VEGF/Ang2 nanobody selectively prevent brain metastases of lung adenocarcinoma cells
Authors: Kovalchuk Bogdana , Berghoff Anna S. , Karreman Matthia A. , Frey Katharina , Piechutta Manuel , Fischer Manuel , Grosch Julia , Heiland Sabine , Breckwoldt Michael O. , Hilberg Frank , Wick Wolfgang , Winkler Frank
Published: September 2020

Brain metastases (BM) are an ever-increasing challenge in oncology, threatening quality of life and survival of many cancer patients. The majority of BM originate from lung adenocarcinoma, and stage III patients have a risk of 40–50% to develop BM in the first years of disease onset. As therapeutic options are limited, prevention of their occurrence is an attractive concept. Here we investigated whether Nintedanib (BIBF 1120), a tyrosine kinase inhibitor (TKI) targeting the VEGF pathway approved for lung adenocarcinoma, and the dual anti-VEGF-A/Ang2 nanobody BI836880 have the potential to prevent BM formation. A mouse model of brain metastasis from lung adenocarcinoma was used in which tumor cells were injected intracardially. Metastases formation occurred inside and outside of the brain and was followed by MRI, IVIS, and immunohistochemistry. BM were reduced in volume and number by both Nintedanib and the dual anti-VEGF-A/Ang2 nanobody, which translated into improved survival. Both compounds were able to normalize cerebral blood vessels at the site of brain metastatic lesions. Extracranial metastases, however, were not reduced, and meningeal metastases only partially. Interestingly, unspecific control IgG also lead to brain vessel normalization and reduction of brain and meningeal metastases. This data indicates a brain-specific group effect of antiangiogenic compounds with respect to metastasis prevention, most likely by preventing an early angiogenic switch. Thus, Nintedanib and BI836880 are promising candidates for future BM preventive study concepts in lung adenocarcinoma patients.
The Cognitive Philosophy of Reflection
Authors: Stephens Andreas , Tjøstheim Trond A.
Published: September 2020

Hilary Kornblith argues that many traditional philosophical accounts involve problematic views of reflection (understood as second-order mental states). According to Kornblith, reflection does not add reliability, which makes it unfit to underlie a separate form of knowledge. We show that a broader understanding of reflection, encompassing Type 2 processes, working memory, and episodic long-term memory, can provide philosophy with elucidating input that a restricted view misses. We further argue that reflection in fact often does add reliability, through generalizability, flexibility, and creativity that is helpful in newly encountered situations, even if the restricted sense of both reflection and knowledge is accepted. And so, a division of knowledge into one reflexive (animal) form and one reflective form remains a plausible, and possibly fruitful, option.
Enabling Exit: Religious Association and Membership Contract
Authors: Rouméas Élise
Published: September 2020

This paper investigates the right of exit from religious associations. The liberal state has a compelling interest in overseeing exit, even if it implies some loss in religious group autonomy. Members should not be bound by rules they find unconscionable. They should be free to leave and able to do so. To enable exit, the paper advocates the use of membership contracts. Religious associations should issue a contract for members working for, residing in, or donating money to the association under a regime of legal exemptions. The membership contract publicises the right of exit and offers a basis for negotiating and contesting its terms. It makes exit less “unthinkable” to members and helps tackle unreasonable economic costs to exit.
Dynamic 15N{1H} NOE measurements: a tool for studying protein dynamics
Authors: Kharchenko Vladlena , Nowakowski Michal , Jaremko Mariusz , Ejchart Andrzej , Jaremko Łukasz
Published: September 2020

Intramolecular motions in proteins are one of the important factors that determine their biological activity and interactions with molecules of biological importance. Magnetic relaxation of 15N amide nuclei allows one to monitor motions of protein backbone over a wide range of time scales. 15N{1H} nuclear Overhauser effect is essential for the identification of fast backbone motions in proteins. Therefore, exact measurements of NOE values and their accuracies are critical for determining the picosecond time scale of protein backbone. Measurement of dynamic NOE allows for the determination of NOE values and their probable errors defined by any sound criterion of nonlinear regression methods. The dynamic NOE measurements can be readily applied for non-deuterated or deuterated proteins in both HSQC and TROSY-type experiments. Comparison of the dynamic NOE method with commonly implied steady-state NOE is presented in measurements performed at three magnetic field strengths. It is also shown that improperly set NOE measurement cannot be restored with correction factors reported in the literature.
Huckleberry Finn’s Conscience: Reckoning with the Evasion
Authors: Clarke Steve
Published: September 2020

Huck Finn’s struggles with his conscience, as depicted in Mark Twain’s famous novelThe Adventures of Huckleberry Finn(AHF) (1884), have been much discussed by philosophers; and various philosophical lessons have been extracted from Twain’s depiction of those struggles. Two of these philosophers stand out, in terms of influence: Jonathan Bennett and Nomy Arpaly. Here I argue that the lessons that Bennett and Arpaly draw are not supported by a careful reading of AHF. This becomes particularly apparent when we consider the final part of the book, commonly referred to, by literary scholars, as ‘the evasion’. During the evasion Huck behaves in ways that are extremely difficult to reconcile with the interpretations of AHF offered by Bennett and Arpaly. I extract a different philosophical lesson from AHF than either Bennett or Arpaly, which makes sense of the presence of the evasion in AHF. This lesson concerns the importance of conscious moral deliberation for moral guidance and for overcoming wrongful moral assumptions. I rely on an interpretation of AHF that is influential in literary scholarship. On it the evasion is understood as an allegory about US race relations during the 20-year period from the end of the US Civil War to the publication of AHF.
Government Revenue and Child and Maternal Mortality
Authors: Hall Stephen , Illian Janine , Makuta Innocent , McNabb Kyle , Murray Stuart , O’Hare Bernadette AM , Python Andre , Zaidi Syed Haider Ali , Bar-Zeev Naor
Published: September 2020

Most maternal and child deaths result from inadequate access to the critical determinants of health: clean water, sanitation, education and healthcare, which are also among the Sustainable Development Goals. Reasons for poor access include insufficient government revenue for essential public services. In this paper, we predict the reductions in mortality rates — both child and maternal — that could result from increases in government revenue, using panel data from 191 countries and a two-way fixed-effect linear regression model. The relationship between government revenue per capita and mortality rates is highly non-linear, and the best form of non-linearity we have found is a version of an inverse function. This implies that countries with small per-capita government revenues have a better scope for reducing mortality rates. However, as per-capita revenue rises, the possible gains decline rapidly in a non-linear way. We present the results which show the potential decrease in mortality and lives saved for each of the 191 countries if government revenue increases. For example, a 10% increase in per-capita government revenue in Afghanistan in 2002 ($24.49 million) is associated with a reduction in the under-5 mortality rate by 12.35 deaths per 1000 births and 13,094 lives saved. This increase is associated with a decrease in the maternal mortality ratio of 9.3 deaths per 100,000 live births and 99 maternal deaths averted. Increasing government revenue can directly impact mortality, especially in countries with low per- capita government revenues. The results presented in this study could be used for economic, social and governance reporting by multinational companies and for evidence-based policymaking and advocacy.
Convergence rates of Gaussian ODE filters
Authors: Kersting Hans , Sullivan T. J. , Hennig Philipp
Published: September 2020

A recently introduced class of probabilistic (uncertainty-aware) solvers for ordinary differential equations (ODEs) applies Gaussian (Kalman) filtering to initial value problems. These methods model the true solutionxand its firstqderivativesa priorias a Gauss–Markov process$${\varvec{X}}$$X, which is then iteratively conditioned on information about$${\dot{x}}$$x˙. This article establishes worst-case local convergence rates of order$$q+1$$q+1for a wide range of versions of this Gaussian ODE filter, as well as global convergence rates of orderqin the case of$$q=1$$q=1and an integrated Brownian motion prior, and analyses how inaccurate information on$${\dot{x}}$$x˙coming from approximate evaluations offaffects these rates. Moreover, we show that, in the globally convergent case, the posterior credible intervals are well calibrated in the sense that they globally contract at the same rate as the truncation error. We illustrate these theoretical results by numerical experiments which might indicate their generalizability to$$q \in \{2,3,\ldots \}$$q∈{2,3,…}.
Multivariate analysis of activated sludge community in full-scale wastewater treatment plants
Authors: Sobczyk Mateusz , Pajdak-Stós Agnieszka , Fiałkowska Edyta , Sobczyk Łukasz , Fyda Janusz
Published: September 2020

We investigated changes in protozoa and metazoa community in relation to process parameters in activated sludge from four wastewater treatment plants (WWTPs) throughout the period of 1 year. Principal component analysis (PCA) showed that activated sludge from investigated treatment plants had different dominating species representatives and community composition mainly depends on individual features of the treatment plants. Redundancy analysis (RDA) showed that the temperature in bioreactors was the most relevant factor explaining changes in the microorganism community, whereas reduction rate of chemical oxygen demand (COD), biological oxygen demand (BOD5), suspended solids (SS), and total nitrogen (TN) did not sufficiently explain the variation in protozoa and metazoan community composition. The results indicate that in stable working WWTP it is difficult to find a pronounced link between activated sludge species composition, process parameters, and plant configuration. Applied multivariate analysis can be a valuable tool for the exploration of the relations between community composition and WWTP process parameters.
Leisure Time Sports Activities and Life Satisfaction: Deeper Insights Based on a Representative Survey from Germany
Authors: Mutz Michael , Reimers Anne K. , Demetriou Yolanda
Published: September 2020

Observational and experimental studies show that leisure time sporting activity (LTSA) is associated with higher well-being. However, scholars often seem to assume that 1) LTSA fosters “general” life satisfaction, thereby ignoring effects on domain satisfaction; 2) the effect of LTSA on well-being is linear and independent of a person’s general activity level; 3) the amount of LTSA is more important than the repertoire of LTSA, i.e. the number of different activities; 4) all kinds of LTSA are equal in their effects, irrespective of spatial and organisational context conditions. Using data from the German SALLSA-Study (“Sport, Active Lifestyle and Life Satisfaction”), a large-scale CAWI-Survey (N = 1008) representing the population ≥ 14 years, the paper takes a closer look on these assumptions. Findings demonstrate that LTSA is associated with general life satisfaction and domain-specific satisfaction (concerning relationships, appearance, leisure, work and health), but that the relationship is most pronounced for leisure satisfaction. Associations of sport with life satisfaction, leisure satisfaction and subjective health are non-linear, approaching an injection point from which on additional LTSA is no longer beneficial. Moreover, findings lend support to the notion that diversity in LTSA matters, as individuals with higher variation in sports activities are more satisfied. Finally, results with regard to spatial and organizational context suggest that outdoor sports and club-organized sports have additional benefits.