bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlation between Carbapenem Consumption and Carbapenems Susceptibility Profiles of Acinetobacter baumannii and Pseudomonas aeruginosa in an Academic Medical Center in Thailand
Authors: Paiboonvong Taniya , Tedtaisong Phatchareeporn , Montakantikul Preecha , Gorsanan Sarun , Tantisiriwat Woraphot
Published: January 2022

The emergent issue of carbapenem-resistant Acinetobacter baumannii (A. baumannii) and Pseudomonas aeruginosa (P. aeruginosa) is a major problem in Thailand. The wide use of carbapenems can increase selective pressure of bacterial resistance. The objective of this study was to determine the relationship between carbapenem consumption and the susceptibility rates of A. baumannii and P. aeruginosa, including multi-drug resistance (MDR) strains. This was a retrospective study. Carbapenem consumption and susceptibility profiles were collected from 2007 to 2013 at the Her Royal Highness Princess Maha Chakri Sirindhorn Medical Center, Thailand. We found that the susceptibility rate of A. baumannii to imipenem and meropenem from the sputum and the bronchoalveolar lavage (BAL) specimens was significantly decreased during the study period, but no significant change was found in the P. aeruginosa data. The relationship between carbapenem consumption and the susceptibility rate of A. baumannii had a clear association with the use of ertapenem. We found a statistically significant negative correlation between ertapenem consumption and the susceptibility rate of A. baumannii to imipenem (r = −0.91; p = 0.004) and meropenem (r = −0.97; p = 0.000) in the data from the non-ICU wards. In addition, imipenem use had a moderate negative correlation with the MDR P. aeruginosa data but no statistical significance (r = −0.714; p > 0.05). In conclusion, our study suggested there is an association between carbapenem use and the susceptibility of A. baumannii and P. aeruginosa. Notwithstanding this, information on ecological factors should be considered for further study. These findings showed the need to optimize the carbapenem prescription policy. Avoiding carbapenem overuse and rethinking the appropriate initial therapy might decrease the rate of resistant organisms.
Inokosterone from Gentiana rigescens Franch Extends the Longevity of Yeast and Mammalian Cells via Antioxidative Stress and Mitophagy Induction
Authors: Liu Yanan , Liu Qian , Chen Danni , Matsuura Akira , Xiang Lan , Qi Jianhua
Published: January 2022

In the present study, replicative lifespan and chronological lifespan assays of yeast were used to double-screen antiaging compounds from Gentiana rigescens Franch, a Chinese herb medicine. Inokosterone from G. rigescens Franch extended not only the replicative lifespan of K6001 yeast but also the chronological lifespan of YOM36 yeast. Furthermore, it can enhance the survival ability of mammalian cells. In order to understand the mechanism of action of this compound, this study focused on antioxidative stress and autophagy when performing the analysis. The increased cell survival rate under oxidative stress conditions, antioxidant enzyme activity and gene expression were observed in the inokosterone-treated groups. Meanwhile, the reactive oxygen species (ROS) and lipid peroxidation of yeast were obviously decreased. Additionally, the macroautophagy and mitophagy in YOM38-GFP-ATG8 yeast were increased upon inokosterone treatment, respectively. At the same time, the cleavage-free GFP from GFP-ATG8 in the cytoplasm and the ubiquitin of the mitochondria at the protein level were markedly enhanced after incubation with inokosterone. Furthermore, we investigated the effect of inokosterone on antioxidative stress and autophagy in mammalian cells, and the relationship between ROS and autophagy. The ROS, malondialdehyde (MDA) were significantly decreased, and the autophagosomes in mammalian cells were obviously increased after inokosterone treatment. The autophagosomes in ∆sod1 yeast with a K6001 background had no obvious changes, and the ROS and MDA of ∆sod1 yeast were increased compared with K6001 yeast. The increase of autophagosomes and the reduction of ROS and MDA in ∆sod1 yeast were observed after treatment with inokosterone. Meanwhile, the reduction of the ROS level and the increase of the SOD1 gene expression of K6001 yeast lacking autophagy were observed after treatment with inokosterone. In order to indicate whether the genes related to antioxidant enzymes and autophagy were involved in the antiaging effect of inokosterone, mutants of K6001 yeast were constructed to conduct a lifespan assay. The replicative lifespans of ∆sod1, ∆sod2, ∆uth1, ∆skn7, ∆gpx, ∆cat, ∆atg2, and ∆atg32 of K6001 yeast were not affected by inokosterone. These results suggest that inokosterone exerted an antiaging activity via antioxidative stress and increased autophagy activation; autophagy affected the ROS levels of yeast via the regulation of SOD1 gene expression.
Anti-Osteoporotic Mechanisms of Polyphenols Elucidated Based on In Vivo Studies Using Ovariectomized Animals
Authors: Niwano Yoshimi , Kohzaki Hidetsugu , Shirato Midori , Shishido Shunichi , Nakamura Keisuke
Published: January 2022

Polyphenols are widely known for their antioxidant activity, i.e., they have the ability to suppress oxidative stress, and this behavior is mediated by the autoxidation of their phenolic hydroxyl groups. Postmenopausal osteoporosis is a common health problem that is associated with estrogen deficiency. Since oxidative stress is thought to play a key role in the onset and progression of osteoporosis, it is expected that polyphenols can serve as a safe and suitable treatment in this regard. Therefore, in this review, we aimed to elucidate the anti-osteoporotic mechanisms of polyphenols reported by in vivo studies involving the use of ovariectomized animals. We categorized the polyphenols as resveratrol, purified polyphenols other than resveratrol, or polyphenol-rich substances or extracts. Literature data indicated that resveratrol activates sirtuin 1, and thereafter, suppresses osteoclastogenic pathways, such as the receptor activator of the nuclear factor kappa B (RANK) ligand (RANKL) pathway, and promotes osteoblastogenic pathways, such as the wingless-related MMTV integration site pathway. Further, we noted that purified polyphenols and polyphenol-rich substances or extracts exert anti-inflammatory and/or antioxidative effects, which inhibit RANKL/RANK binding via the NF-κB pathway, resulting in the suppression of osteoclastogenesis. In conclusion, antioxidative and anti-inflammatory polyphenols, including resveratrol, can be safe and effective for the treatment of postmenopausal osteoporosis based on their ability to regulate the imbalance between bone formation and resorption.
Compact Broadband Antenna with Vicsek Fractal Slots for WLAN and WiMAX Applications
Authors: Benkhadda Omaima , Ahmad Sarosh , Saih Mohamed , Chaji Kebir , Reha Abdelati , Ghaffar Adnan , Khan Salahuddin , Alibakhshikenari Mohammad , Limiti Ernesto
Published: January 2022

This paper aims to design a compact broadband antenna for wireless local area network (WLAN) and worldwide interoperability for microwave access (WIMAX) applications. The suggested antenna consists of an octagonal radiator with Vicsek fractal slots and a partial ground plane, it is printed on FR-4 dielectric substrate, and its global dimension is 50 × 50 × 1.6 mm3. The antenna is designed and constructed using both CST MICROWAVE STUDIO® and CADFEKO electromagnetic solver, and in order to validate the acquired simulation results, the antenna is manufactured and tested using vector network analyzer E5071C. The measurement results show that the designed antenna attains a broadband bandwidth (S11 < −10 dB) from 2.48 to 6.7 GHz resonating at 3.6 and 5.3 GHz, respectively. The broadband bandwidth covers the two required bands: WiMAX at the frequencies 2.3/2.5/3.3/3.5/5/5.5 GHz and WLAN at the frequencies 3.6/2.4–2.5/4.9–5.9 GHz. In addition, the suggested antenna provides good gains of 2.78 dBi and 5.32 dBi, omnidirectional measured radiation patterns in the E-plane and the H-plane and high efficiencies of 88.5% and 84.6% at the resonant frequencies. A close agreement of about 90% between simulation and measurement results is noticed.
Probiotic Molecules That Inhibit Inflammatory Diseases
Authors: Zamora-Pineda Jesus , Kalinina Olga , Osborne Barbara A. , Knight Katherine L.
Published: January 2022

Consumption of probiotics for health purposes has increased vastly in the past few decades, and yet the scientific evidence to support health benefits from probiotics is only beginning to emerge. As more probiotics are studied, we are beginning to understand the mechanisms of action by which they benefit human health, as well as to identify the bacterial molecules responsible for these benefits. A new era of therapeutics is on the horizon in which purified molecules from probiotics will be used to prevent and treat diseases. In this review, we summarize the active molecules from probiotic bacteria that have been shown to affect innate and adaptive immunity and have health benefits in experimental settings. We focus particularly on the cellular and molecular mechanisms of the probiotic Bacillus subtilis and its active molecule, exopolysaccharide (ESPBs).
Enhanced Proton Acceleration from Laser Interaction with a Tailored Nanowire Target
Authors: Chao Yue , Cao Lihua , Zheng Chunyang , Liu Zhanjun , He Xiantu
Published: January 2022

Target normal sheath field acceleration via laser interaction with structured solid targets has been widely studied for its potential use in a wide range of applications. Here, a novel nanowire target with a corrugated front surface is proposed to improve the proton acceleration by a target normal sheath field. Two-dimensional particle-in-cell simulations demonstrated that with the existence of the corrugated surface, the cut-off energy of accelerated protons nearly doubles compared to the planar nanowire target. When interacting with the corrugated surface, the incident laser pulse is reflected multiple times, focused and reinforced in each cavity near the front surface, which leads to suppression of the reflectivity and an improvement in the absorption rate. Electrons are heated more efficiently and the sheath field at the target rear side is naturally enhanced. To further investigate the performance of this novel target, a series of simulations with various laser intensities and target sizes were also carried out. This simple target design may provide insights for experiments in the future and should arouse interest because of its wide application.
The Effect of Under-Drilling and Osseodensification Drilling on Low-Density Bone: A Comparative Ex Vivo Study
Authors: Seo Dong-Jun , Moon Seong-Yong , You Jae-Seek , Lee Won-Pyo , Oh Ji-Su
Published: January 2022

The aim of this study is to identify a method that can maximize implant primary stability (IPS) and bone density under the controlled drilling conditions of the same diameter and length in low-density bones through an ex vivo study. A total of 87 dental implants were placed with standard drilling, under-drilling, and osseodensification drilling in 13 fresh porcine sternums. The Periotest value and the implant stability quotient were measured to evaluate the primary stability. The difference in the Hounsfield unit (HU) between the hole and peripheral bone up to a distance of 1 mm was measured. Osseodensification and under-drilling technique increased the IPS, compared with conventional drilling technique with statistical significance under the drilling conditions of the same diameter and length. Osseodensification technique with the counter-clockwise direction had higher HU gaps than the standard drilling and osseodensification technique with clockwise direction. Due to the effect of bone densification, the gap of HU was increased by a minimum of 43 HU and a maximum of 180 HU. Within the limitations of this ex vivo study, it was found that the osseodensification technique with counter-clockwise direction is effective to increase IPS and bone mineral density in low-density bone.
Application of VR Technology to the Training of Paramedics
Authors: Boros Martin , Sventekova Eva , Cidlinova Anna , Bardy Marek , Batrlova Katerina
Published: January 2022

The virtual world has long been a focus not only of the gaming sphere, but also of the manufacturing and educational industries. The virtual world and its technology have many advantages, the basic ones being, for example, the use of experiential learning, with which the human brain can remember some things better and faster. It was due to the advantages of virtual reality technology that we decided to create an educational system on safety and health at work, and we focused on the healthcare segment due to the COVID-19 pandemic. Thanks to the cooperation of a professional consortium, we created an educational system for safety and health at work and carried out several extensive laboratory measurements, the results of which we followed up in practical measurements with medical staff. The created system is inherently unique and applicable and can be used across several industries. The article presents three basic types of scenarios as well as an evaluation of satisfaction with the proposed system from test participants, i.e., nurses.
Influence of MnO2-Birnessite Microstructure on the Electrochemical Performance of Aqueous Zinc Ion Batteries
Authors: López María Luisa , Álvarez-Serrano Inmaculada , Giraldo David Agustin , Almodóvar Paloma , Rodríguez-Aguado Elena , Rodríguez-Castellón Enrique
Published: January 2022

KxMnO2 materials with birnessite-type structure are synthetized by two different methods which make it possible to obtain manganese oxides with different degrees of crystallinity. The XPS results indicate that the sample obtained at high temperature (KMn8) exhibits a lower oxidation state for manganese ions as well as a denser morphology. Both characteristics could explain the lower capacity value obtained for this electrode. In contrast, the sample obtained at low temperature (KMn4) or by hydrothermal method presents a manganese oxidation state close to 4 and a more porous morphology. Indeed, in this case higher capacity values are obtained. At current density of 30 mA g−1, the KMn8, KMn4, and HKMn samples display a capacity retention of 88, 82, and 68%, respectively. The higher capacity loss obtained for the HKMn compound could be explained considering that the incorporation of Zn2+ in the structure gives rise to the stabilization of a ZnMn2O4 spinel-type phase. This compound is obtained in the discharge process but remains in the charge stage. Thus, when this spinel-type phase is obtained the capacity loss increases. Moreover, the stabilization of this phase is more favorable at low current rates where 100% of retention for all samples, before 50 cycles, was observed.
Analyses of Xylem Vessel Size on Grapevine Cultivars and Relationship with Incidence of Esca Disease, a Threat to Grape Quality
Authors: Foglia Renzo , Landi Lucia , Romanazzi Gianfranco
Published: January 2022

Esca disease is one of the most important grapevine trunk diseases. It seriously reduces the quality and quantity of grapevine production, and results in a shorter vineyard lifespan. Previous studies have suggested that wide xylem vessel diameter favours development on grapevine of Phaeomoniella chlamydospora, one of the fungi involved in esca, thus affecting disease susceptibility. In this study, cultivars mainly originated from European countries, 27 white-berried and 24 red-berried grapevine cultivars, were grown in the same experimental vineyard and were analysed for xylem vessel sizes (as diameter and frequency) for correlation with esca incidence. In this study, the cultivars showed significant differences in the xylem vessel parameters. However, no relationship was detected between vessel size and esca incidence in the field. Overall, white-berried cultivars showed wider vessel diameters than red-berried cultivars. The relationship between xylem vessel size in the red-berried and white-berried cultivars and incidence of esca symptoms is discussed. We suggest that vessel anatomy profiles can provide useful information for further investigations on grapevine genotype structure–esca incidence relationships.
Grapevine DMR6-1 Is a Candidate Gene for Susceptibility to Downy Mildew
Authors: Pirrello Carlotta , Malacarne Giulia , Moretto Marco , Lenzi Luisa , Perazzolli Michele , Zeilmaker Tieme , Van den Ackerveken Guido , Pilati Stefania , Moser Claudio , Giacomelli Lisa
Published: January 2022

Grapevine (Vitis vinifera) is a valuable crop in Europe for both economical and cultural reasons, but highly susceptible to Downy mildew (DM). The generation of resistant vines is of critical importance for a sustainable viticulture and can be achieved either by introgression of resistance genes in susceptible varieties or by mutation of Susceptibility (S) genes, e.g., by gene editing. This second approach offers several advantages: it maintains the genetic identity of cultivars otherwise disrupted by crossing and generally results in a broad-spectrum and durable resistance, but it is hindered by the poor knowledge about S genes in grapevines. Candidate S genes are Downy mildew Resistance 6 (DMR6) and DMR6-Like Oxygenases (DLOs), whose mutations confer resistance to DM in Arabidopsis. In this work, we show that grapevine VviDMR6-1 complements the Arabidopsis dmr6-1 resistant mutant. We studied the expression of grapevine VviDMR6 and VviDLO genes in different organs and in response to the DM causative agent Plasmopara viticola. Through an automated evaluation of causal relationships among genes, we show that VviDMR6-1, VviDMR6-2, and VviDLO1 group into different co-regulatory networks, suggesting distinct functions, and that mostly VviDMR6-1 is connected with pathogenesis-responsive genes. Therefore, VviDMR6-1 represents a good candidate to produce resistant cultivars with a gene-editing approach.
Marine Origin Ligands of Nicotinic Receptors: Low Molecular Compounds, Peptides and Proteins for Fundamental Research and Practical Applications
Authors: Kasheverov Igor , Kudryavtsev Denis , Shelukhina Irina , Nikolaev Georgy , Utkin Yuri , Tsetlin Victor
Published: January 2022

The purpose of our review is to briefly show what different compounds of marine origin, from low molecular weight ones to peptides and proteins, offer for understanding the structure and mechanism of action of nicotinic acetylcholine receptors (nAChRs) and for finding novel drugs to combat the diseases where nAChRs may be involved. The importance of the mentioned classes of ligands has changed with time; a protein from the marine snake venom was the first excellent tool to characterize the muscle-type nAChRs from the electric ray, while at present, muscle and α7 receptors are labeled with the radioactive or fluorescent derivatives prepared from α-bungarotoxin isolated from the many-banded krait. The most sophisticated instruments to distinguish muscle from neuronal nAChRs, and especially distinct subtypes within the latter, are α-conotoxins. Such information is crucial for fundamental studies on the nAChR revealing the properties of their orthosteric and allosteric binding sites and mechanisms of the channel opening and closure. Similar data are provided by low-molecular weight compounds of marine origin, but here the main purpose is drug design. In our review we tried to show what has been obtained in the last decade when the listed classes of compounds were used in the nAChR research, applying computer modeling, synthetic analogues and receptor mutants, X-ray and electron-microscopy analyses of complexes with the nAChRs, and their models which are acetylcholine-binding proteins and heterologously-expressed ligand-binding domains.
Yuanhuacin and Related Anti-Inflammatory and Anticancer Daphnane Diterpenes from Genkwa Flos—An Overview
Authors: Bailly Christian
Published: January 2022

The dried flower buds of the plant Daphne genkwa Sieb. et Zucc. have been largely used in traditional Chinese medicine for the treatment of inflammatory diseases. Numerous diterpenoids have been isolated from the Genkwa Flos (yuanhua in Chinese), including a series of daphnane-type diterpene designated as yuanhuacin (YC, often improperly designated as yuanhuacine) and analogues with a patronymic name. The series includes ten daphnane-type diterpenes: yuanhuacin, yuanhuadin (YD), yuanhuafin (YF), yuanhuagin (YG), yuanhuahin (YH), yuanhuajin (YJ), yuanhualin (YL), yuanhuamin (YM), yuanhuapin (YP), and yuanhuatin (YT). They are distinct from the rare flavonoid yuanhuanin. The series comprises several anticancer agents, such as the lead compound YC, which has revealed potent activity in vitro and in vivo against models of lung and breast cancers. The main signaling pathways implicated in the antitumor effects have been delineated. Protein kinase C is a key factor of activity for YC, but in general the molecular targets at the origin of the activity of these compounds remain little defined. Promising anticancer effects have been reported with analogues YD and YT, whereas compounds YF and YP are considered more toxic. The pharmacological activity of each compound is presented, as well as the properties of Genkwa Flos extracts. The potential toxic effects associated with the use of these compounds are also underlined.
Photodynamic Effects with 5-Aminolevulinic Acid on Cytokines and Exosomes in Human Peripheral Blood Mononuclear Cells
Authors: Espeland Kristian , Kleinauskas Andrius , Juzenas Petras , Brech Andreas , Darvekar Sagar , Vasovic Vlada , Warloe Trond , Christensen Eidi , Jahnsen Jørgen , Peng Qian
Published: January 2022

Photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA), a precursor to the potent photosensitizer, protoporphyrin IX (PpIX), is an established modality for several malignant and premalignant diseases. This treatment is based on the light-activated PpIX in targeted lesions. Although numerous studies have confirmed the necrosis and apoptosis involved in the mechanism of action of this modality, little information is available for the change of exosome levels after treatment. We report from the first study on the effects of ALA-PDT on cytokines and exosomes of human healthy peripheral blood mononuclear cells (PBMCs). The treatment reduced the cytokines and exosomes studied, although there was variation among individual PBMC samples. This reduction is consistent with PDT-mediated survivals of subsets of PBMCs. More specifically, the ALA-PDT treatment apparently decreased all pro-inflammatory cytokines included, suggesting that this treatment may provide a strong anti-inflammatory effect. In addition, the treatment has decreased the levels of different types of exosomes, the HLA-DRDPDQ exosome in particular, which plays an important role in the rejection of organ transplantation as well as autoimmune diseases. These results may suggest future therapeutic strategies of ALA-PDT.
Synergy of Human Platelet-Derived Extracellular Vesicles with Secretome Proteins Promotes Regenerative Functions
Authors: Gomes Fausto Gueths , Andrade André Cronemberger , Wolf Martin , Hochmann Sarah , Krisch Linda , Maeding Nicole , Regl Christof , Poupardin Rodolphe , Ebner-Peking Patricia , Huber Christian G. , Meisner-Kober Nicole , Schallmoser Katharina , Strunk Dirk
Published: January 2022

Platelet-rich plasma is a promising regenerative therapeutic with controversial efficacy. We and others have previously demonstrated regenerative functions of human platelet lysate (HPL) as an alternative platelet-derived product. Here we separated extracellular vesicles (EVs) from soluble factors of HPL to understand the mode of action during skin-organoid formation and immune modulation as model systems for tissue regeneration. HPL-EVs were isolated by tangential-flow filtration (TFF) and further purified by size-exclusion chromatography (SEC) separating EVs from (lipo)protein-enriched soluble fractions. We characterized samples by tunable resistive pulse sensing, western blot, tandem mass-tag proteomics and super-resolution microscopy. We evaluated EV function during angiogenesis, wound healing, organoid formation and immune modulation. We characterized EV enrichment by TFF and SEC according to MISEV2018 guidelines. Proteomics showed three major clusters of protein composition separating TSEC-EVs from HPL clustering with TFF soluble fractions and TFF-EVs clustering with TSEC soluble fractions, respectively. HPL-derived TFF-EVs promoted skin-organoid formation and inhibited T-cell proliferation more efficiently than TSEC-EVs or TSEC-soluble fractions. Recombining TSEC-EVs with TSEC soluble fractions re-capitulated TFF-EV effects. Zeta potential and super-resolution imaging further evidenced protein corona formation on TFF-EVs. Corona depletion on SEC-EVs could be artificially reconstituted by TSEC late fraction add-back. In contrast to synthetic nanoparticles, which commonly experience reduced function after corona formation, the corona-bearing EVs displayed improved functionality. We conclude that permissive isolation technology, such as TFF, and better understanding of the mechanism of EV corona function are required to realize the complete potential of platelet-based regenerative therapies.
Differential Expression of Genes Involved in Metabolism and Immune Response in Diffuse and Intestinal Gastric Cancers, a Pilot Ptudy
Authors: Perrot-Applanat Martine , Pimpie Cynthia , Vacher Sophie , Bieche Ivan , Pocard Marc , Baud Véronique
Published: January 2022

Gastric cancer (GC) is one of the major causes of cancer-related mortality worldwide. The vast majority of GC cases are adenocarcinomas including intestinal and diffuse GC. The incidence of diffuse GCs, often associated with poor overall survival, has constantly increased in USA and Europe The molecular basis of diffuse GC aggressivity remains unclear. Using mRNA from diffuse and intestinal GC tumor samples of a Western cohort, this study reports the expression level of the immunomodulatory aryl-hydrocarbon receptor (AhR), and genes involved in immune suppression (PD1, PD-L1, PD-L2) and the early steps of tryptophan metabolism (IDO1, IDO2, TDO2). Strongly increased expression of IDO1 (p < 0.001) and PD1 (p < 0.003) was observed in the intestinal sub-type. The highest expression of IDO1 and PDL1 correlated with early clinical stage and absence of lymphatic invasion (×25 p = 0.004, ×3 p = 0.04, respectively). Our results suggest that kynurenine, produced by tryptophan catabolism, and AhR activation play a central role in creating an immunosuppressive environment. Correspondingly, as compared to intestinal GCs, expression levels of IDO1-TDO2 and PD-L1 were less prominent in diffuse GCs which also had less infiltration of immune cells, suggesting an inactive immune response in the advanced diffuse GC. Confirmation of these patterns of gene expression will require a larger cohort of early and advanced stages of diffuse GC samples.
Resilience in People with Lewy Body Disorders and Their Care Partners: Association with Mental Health, Relationship Satisfaction, and Care Burden
Authors: Vatter Sabina , Leroi Iracema
Published: January 2022

The emergence of cognitive impairment and dementia in people with Lewy body spectrum disorders (LBS) significantly impacts the quality of life of the individual and their care partner. Coping well with the condition may depend, in part, on the degree of psychological resilience or capacity to ‘bounce back’ from adversity. We explored resilience in people with Parkinson’s disease mild cognitive disorder or dementia, or dementia with Lewy bodies, and their care partners, and its relation to outcomes related to their mental well-being and quality of life. This exploratory, cross-sectional study recruited 76 participant-dyads. Resilience, quality of life, depression, anxiety, and relationship satisfaction were evaluated in both members of the dyad. In care partners, care burden and stress were also assessed. Over 70% of both care partners and recipients reported high levels of resilience. Lower resilience in both members of the dyad was associated with higher anxiety and lower quality of life. Additionally, lower resilience in care partners was associated with lower well-being, relationship satisfaction, and higher burden and stress. Resilience in persons with LBS and their care partners is important to consider when assessing mental health, relationship, and care burden outcomes, acting as a focus of intervention to support positive outcomes.
A Study on the Mechanical Properties and Microcosmic Mechanism of Basalt Fiber Modified Rubber Ceramsite Concrete
Authors: Bu Changming , Zhu Dongxu , Liu Lei , Lu Xinyu , Sun Yi , Yan Zhitao , Yu Linwen , Wei Qike
Published: January 2022

In order to solve the problem of black pollution in the 21st century, a new type of rubber ceramsite concrete was prepared by pulverizing waste rubber tires into granules and adding aggregate to the ceramsite concrete. In order to reduce the weakening of mechanical properties of rubber, basalt fiber was used to modify the rubber. In this study, the compressive strength and splitting tensile strength of concrete specimens at days 3 and 28 were measured, the variation rule of compressive strength was explained based on the appearance of the microstructure, and the internal porosity of cubic specimens was characterized by the water absorption size. At the same time, an ultrasonic wave velocity test was used to test the uniformity and pore distribution of the block. The results showed that, with the increase in the rubber content, the compressive strength of rubber ceramsite concrete increases first and then decreases. The addition of basalt fiber can improve the compressive strength of the concrete. Water absorption is negatively correlated with compressive strength. Porosity is also negatively correlated with ultrasonic wave velocity. The basalt fiber length has no significant effect on the splitting tensile strength.
Design Optimization of Hyperboloid Wooden House Concerning Structural, Cost, and Daylight Performance
Authors: Khidmat Rendy Perdana , Fukuda Hiroatsu , Kustiani
Published: January 2022

The use of parametric and multi-objective optimization (MOO) as a new way of approaching architectural design has been growing in line with current breakthroughs in computational architecture. Wood, on the other hand, is a living and unique building material that provides durability, manufacturing flexibility, and local availability. One of the structure types that provides high structural stability is the hyperboloid. However, the exploration of hyperboloid structures in building design, together with the building daylight objective, is still limitedly reported. This paper presents the application of the parametric approach and multi-objective optimization in optimizing the structure and daylight objectives of a hyperboloid two-story wooden house in Japan, made of 105 mm × 105 mm × 4000 mm Japanese timber. The method involves iterating dynamic parameters such as radius bottom, offset distance, timber members, twisting level, building height, radius-top, and roof slope to optimize the structural objective of minimizing normal force average, displacement, and cost while simultaneously maximizing building volume. Regarding daylight objectives, unit movement and glazing ratio that control the glazing strategies were explored to optimize useful daylight illumination (UDI) in summer and winter. The optimization and exploration yielded 10,098 solutions in structural analysis and 406 solutions in daylight exploration. Based on the data analysis, the proposed methodology has successfully produced the best design solution, discovering the balance between the objective trade-offs. In addition, the most influential parameter that shapes the value of design objectives has been identified. The findings of this research were expected to contribute to and enhance the performance-based design optimization, and support design decision-making process in the early design stage of a wooden house with a hyperboloid structure.
Additive Intralesional Interleukin-2 Improves Progression-Free Survival in a Distinct Subgroup of Melanoma Patients with Prior Progression under Immunotherapy
Authors: Rafei-Shamsabadi David , Lehr Saskia , Behrens Max , Meiss Frank
Published: January 2022

A considerable amount of melanoma patients show primary resistance to PD-1 and CTLA-4 inhibitors. We have previously reported a beneficial role of intralesional Interleukin-2 (IL-2) in 9 melanoma patients developing new locoregional metastases under immunotherapy. We have now expanded this retrospective cohort to 27 patients. Patients were evaluated for their tumor characteristics, treatment response and progression-free and overall survival (PFS/OS). In 16 patients, tumor biopsies before and under IL-2 treatment were evaluated for immune markers. The median follow-up time was 16 (1–59) months from start of IL-2 treatment. Treatment response of locoregional metastases was seen in 74% of all patients and response of distant organ metastases in 37% of stage IV patients, respectively. A prolonged PFS and OS was significantly associated with absence of active distant metastases (p = 0.008), response of locoregional metastases (p = 0.002), increase of absolute eosinophil count (AEC) (p < 0.001) and an influx of CD8+ tumor infiltrating lymphocytes (TILs) (p = 0.003). Additional intralesional treatment with IL-2 in patients with locoregional progression under immunotherapy is a well-tolerated, easily feasible therapeutic option especially in patients lacking active distant metastases. A careful patient selection can lead to an improved PFS and OS.
Combined Fluorescence-Guided Resection and Intracavitary Thermotherapy with Superparamagnetic Iron-Oxide Nanoparticles for Recurrent High-Grade Glioma: Case Series with Emphasis on Complication Management
Authors: Schwake Michael , Müther Michael , Bruns Ann-Katrin , Zinnhardt Bastian , Warneke Nils , Holling Markus , Schipmann Stephanie , Brokinkel Benjamin , Wölfer Johannes , Stummer Walter , Grauer Oliver
Published: January 2022

Background: Concepts improving local tumor control in high-grade glioma (HGG) are desperately needed. The aim of this study is to report an extended series of cases treated with a combination of 5-ALA-fluorescence-guided resection (FGR) and intracavitary thermotherapy with superparamagnetic iron oxide nanoparticles (SPION). Methods: We conducted a single-center retrospective review of all recurrent HGG treated with FGR and intracavitary thermotherapy (n = 18). Patients underwent six hyperthermia sessions in an alternating magnetic field and received additional adjuvant therapies on a case-by-case basis. Results: Nine patients were treated for first tumor recurrence; all other patients had suffered at least two recurrences. Nine patients received combined radiotherapy and thermotherapy. The median progression-free survival was 5.5 (95% CI: 4.67–6.13) months and median overall survival was 9.5 (95% CI: 7.12–11.79) months. No major side effects were observed during active treatment. Thirteen patients (72%) developed cerebral edema and more clinical symptoms during follow-up and were initially treated with dexamethasone. Six (33%) of these patients underwent surgical removal of nanoparticles due to refractory edema. Conclusions: The combination of FGR and intracavitary thermotherapy with SPION provides a new treatment option for improving local tumor control in recurrent HGG. The development of cerebral edema is a major issue requiring further refinements of the treatment protocol.
Slip versus Slop: A Head-to-Head Comparison of UV-Protective Clothing to Sunscreen
Authors: Berry Elizabeth G. , Bezecny Joshua , Acton Michael , Sulmonetti Taylor P. , Anderson David M. , Beckham Haskell W. , Durr Rebecca A. , Chiba Takahiro , Beem Jennifer , Brash Douglas E. , Kulkarni Rajan , Cassidy Pamela B. , Leachman Sancy A.
Published: January 2022

Ultraviolet radiation (UVR) exposure is the most important modifiable risk factor for skin cancer development. Although sunscreen and sun-protective clothing are essential tools to minimize UVR exposure, few studies have compared the two modalities head-to-head. This study evaluates the UV-protective capacity of four modern, sun-protective textiles and two broad-spectrum, organic sunscreens (SPF 30 and 50). Sun Protection Factor (SPF), Ultraviolet Protection Factor (UPF), Critical Wavelength (CW), and % UVA- and % UVB-blocking were measured for each fabric. UPF, CW, % UVA- and % UVB-blocking were measured for each sunscreen at 2 mg/cm2 (recommended areal density) and 1 mg/cm2 (simulating real-world consumer application). The four textiles provided superior UVR protection when compared to the two sunscreens tested. All fabrics blocked erythemogenic UVR better than the sunscreens, as measured by SPF, UPF, and % UVB-blocking. Each fabric was superior to the sunscreens in blocking full-spectrum UVR, as measured by CW and % UVA-blocking. Our data demonstrate the limitations of sunscreen and UV-protective clothing labeling and suggest the combination of SPF or UPF with % UVA-blocking may provide more suitable measures for broad-spectrum protection. While sunscreen remains an important photoprotective modality (especially for sites where clothing is impractical), these data suggest that clothing should be considered the cornerstone of UV protection.
Outcome of SARS-CoV-2-Infected Polish Patients with Chronic Lymphocytic Leukemia
Authors: Puła Bartosz , Pruszczyk Katarzyna , Pietrusza Ewa , Morawska Marta , Piszczek Weronika , Kalicińska Elżbieta , Szeremet Agnieszka , Tryc-Szponder Jagoda , Wąsik-Szczepanek Ewa , Drozd-Sokołowska Joanna , Krzemień Helena , Rejus Aleksandra , Gajewska Małgorzata , Wiśniewski Kamil , Wysocki Maciej , Majeranowski Alan , Paszkiewicz-Kozik Ewa , Steckiewicz Paweł , Szukalski Łukasz , Bołkun Łukasz , Długosz-Danecka Monika , Giannopoulos Krzysztof , Jamroziak Krzysztof , Lech-Marańda Ewa , Hus Iwona
Published: January 2022

Background. The severe acute respiratory syndrome coronavirus (SARS-CoV-2) has become the cause of a worldwide pandemic, and its clinical infection course in patients with hematological malignancies may be severe. Methods. We performed a retrospective study on 188 chronic lymphocytic leukemia patients (CLL) with COVID-19 infection. Results. At the time of infection 51 patients (27.1%) were treated with Bruton tyrosine kinase inhibitor (BTKi), 46 (24.5%) with anti-CD20 antibodies while 37 patients (19.7%) received venetoclax. In total, 111 patients (59.0%) required hospitalization and 50 patients (26.5%) died due to COVID-19. Patients with poor performance status (ECOG >1; p = 0.02), advanced age (>65 years; p = 0.04), low hemoglobin concentration (≤10 g/dl; p = 0.0001), low platelets (<100 × 109/L; p = 0.003), and elevated lactate dehydrogenase level (LDH; p = 0.014) had an increased risk of death due to COVID-19. Neither CLL treatment status (treatment naïve vs. treated) nor the type of CLL-directed treatment had impact on the SARS-CoV-2 related risk of death. The multivariate survival analysis showed that advanced age (p = 0.009) and low platelet count (p = 0.0001) were associated with significantly shorter patients’ overall survival. Conclusions. SARS-CoV-2 infection in CLL patients is associated with poor outcome regardless of administered CLL-directed treatment.
A Phase II Study to Compare the Safety and Efficacy of Direct Oral Anticoagulants versus Subcutaneous Dalteparin for Cancer-Associated Venous Thromboembolism in Patients with Advanced Upper Gastrointestinal, Hepatobiliary and Pancreatic Cancer: PRIORITY
Authors: Kim Jwa Hoon , Yoo Changhoon , Seo Seyoung , Jeong Jae Ho , Ryoo Baek-Yeol , Kim Kyu-pyo , Lee Jung Bok , Lee Keun-Wook , Kim Ji-Won , Kim Il-Hwan , Kang Myoungjoo , Ryu Hyewon , Cheon Jaekyung , Park Sook Ryun
Published: January 2022

Background: We evaluated the safety and efficacy of direct oral anticoagulants (DOACs) versus subcutaneous dalteparin for cancer-associated venous thromboembolism (CA-VTE) in patients with advanced upper gastrointestinal (GI) tract, hepatobiliary, or pancreatic cancer. Methods: This was a multicenter, randomized, open-label, phase II trial in five centers. Patients randomly received rivaroxaban (15 mg twice daily for 3 weeks, then 20 mg once daily)/apixaban (10 mg twice daily for the first 7 days, then 5 mg twice daily) or dalteparin (200 IU/kg once daily for the first month, then 150 IU/kg once daily). Randomization was stratified by the Eastern Cooperative Oncology Group Performance Status, primary cancer type, active chemotherapy, and participating centers. The primary endpoint was the rates of clinically relevant bleeding (CRB) in the full analysis set (FAS). Results: A total of 90 patients were randomly assigned to the DOAC (n = 44) and dalteparin groups (n = 46) in FAS. CRB and major bleeding (MB) rates were 34.1% and 13.0% (p = 0.018) and 18.2% and 4.3% (p = 0.047) for the DOAC and dalteparin groups, respectively. Time to CRB and MB was higher in the DOAC group than in the dalteparin group (hazard ratio [HR] 2.83; p = 0.031 and HR 4.32; p = 0.064). Cancer involvement at the GI mucosa was also a significant risk factor for CRB. Recurrent CA-VTE occurred in 2.3% and 2.2% of patients given DOAC and dalteparin, respectively (p = 1.000). Conclusion: DOAC therapy further increased the risk of bleeding compared with dalteparin in patients with active advanced upper GI tract, hepatobiliary, or pancreatic cancer, suggesting that extra caution should be taken when selecting anticoagulants for CA-VTE.
Oral Contraceptive Use and Breast Cancer Risk According to Molecular Subtypes Status: A Systematic Review and Meta-Analysis of Case-Control Studies
Authors: Barańska Agnieszka , Dolar-Szczasny Joanna , Kanadys Wiesław , Kinik Wiktoria , Ceglarska Dorota , Religioni Urszula , Rejdak Robert
Published: January 2022

We conducted a systematic review and meta-analysis to investigate the effect of oral contraceptives (OCs) on risk of breast cancer (BrCa) by status of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). We searched the MEDLINE (PubMed), Embase and the Cochrane Library database and bibliographies of pertinent articles published up to 2020. Therein, we identified nineteen eligible case-control studies which provided data by breast cancer subtypes: ER-positive (ER+), ER-negative (ER−), HER2-positive (HER2+) and Triplet-negative (TN). Summary risk estimates (pooled OR [pOR]) and 95% confidence intervals (CIs) were calculated using fixed/random effects models. The summary meta-analysis showed that over-use of OCs led to significant increased risk of TNBrCa (OR = 1.37, 95% CI; 1.13 to 1.67, p = 0.002), as well as of ER−BrCa (OR = 1.20, 95% CI: 1.03 to 1.40, p = 0.019). There was also a significant reduction in the risk of ER+BrCa (OR = O.92, 95% CI: 0.86 to 0.99, p = 0.026,) and a slight reduction in the risk of HER2+BrCa (OR = 0.95, 95% CI; 0.79 to 1.14, p = 0.561) after taking OCs. Meta-analysis indicated that OC use has different impacts on risk of breast cancer subtypes defined by receptor status. The identified differences between individual subtypes of breast cancer may reflect different mechanisms of carcinogenesis.
A Short Review of Aerobic Oxidative Desulfurization of Liquid Fuels over Porous Materials
Authors: Yuan Bo , Li Xiaolin , Sun Yinyong
Published: January 2022

Oxidative desulfurization (ODS) has attracted much attention owing to the mild working conditions and effective removal of the aromatic sulfur-containing compounds which are difficult to desulfurize using the industrial hydrodesulfurization (HDS) technique. Molecular oxygen in ambient air have been recognized as an ideal oxidant in ODS due to its easy availability, non-toxicity and low cost in recent years. However, molecular oxygen activation under mild operating conditions is still a challenge. Porous materials and their composites have drawn increasing attention due to their advantages, such as high surface area and confined pore space, along with their stability. These merits contribute to the fast diffusion of oxygen molecules and the formation of more exposed active sites, which make them ideal catalysts for aerobic oxidation reactions. The confined space pore size offers a means of catalytic activity and durability improvement. This gives rise to copious attention toward the porous catalysts in AODS. In this review, the progress in the characteristics and AODS catalytic activities of porous catalysts is summarized. Then, emphasis on the molecular oxygen activation mechanism is traced. Finally, the breakthroughs and challenges of various categories of porous catalysts are concluded.
Metal–Organic Frameworks (MOFs) and Materials Derived from MOFs as Catalysts for the Development of Green Processes
Authors: Valdebenito Gonzalo , Gonzaléz-Carvajal Marco , Santibañez Luis , Cancino Patricio
Published: January 2022

This review will be centered around the work that has been reported on the development of metal–organic frameworks (MOFs) serving as catalysts for the conversion of carbon dioxide into short-chain hydrocarbons and the generation of clean energies starting from biomass. MOFs have mainly been used as support for catalysts or to prepare catalysts derived from MOFs (as sacrifice template), obtaining interesting results in the hydrogenation or oxidation of biomass. They have presented a good performance in the hydrogenation of CO2 into light hydrocarbon fuels. The common patterns to be considered in the performance of the catalysts are the acidity of MOFs, metal nodes, surface area and the dispersion of the active sites, and these parameters will be discussed in this review.
Golgi Apparatus Regulates Plasma Membrane Composition and Function
Authors: Agliarulo Ilenia , Parashuraman Seetharaman
Published: January 2022

Golgi apparatus is the central component of the mammalian secretory pathway and it regulates the biosynthesis of the plasma membrane through three distinct but interacting processes: (a) processing of protein and lipid cargoes; (b) creation of a sharp transition in membrane lipid composition by non-vesicular transport of lipids; and (c) vesicular sorting of proteins and lipids at the trans-Golgi network to target them to appropriate compartments. We discuss the molecules involved in these processes and their importance in physiology and development. We also discuss how mutations in these molecules affect plasma membrane composition and signaling leading to genetic diseases and cancer.