bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence of Epiretinal Membrane among Subjects in a Health Examination Program in Japan
Authors: Shimizu Hiroshi , Asaoka Ryo , Omoto Takashi , Fujino Yuri , Mitaki Shingo , Onoda Keiichi , Nagai Atsushi , Yamaguchi Shuhei , Tanito Masaki
Published: January 2021

The prevalence of an epiretinal membrane (ERM) was elucidated using a dataset from a health examination program database in Japan. From the cohort database, 5042 eyes of 2552 subjects were included. The presence of an ERM, cellophane macular reflex (CMR), or preretinal macular fibrosis (PMF) was detected using color fundus photographs, and crude and age-standardized prevalence were obtained. To further assess the possible risk factors of ERM, background parameters were compared between ERM+ and − groups, and multiple logistic regression analysis was performed. ERM was detected in fundus photographs of 275 eyes (eye-based prevalence of 5.5%) from 217 subjects (subject-based prevalence of 8.5%). CMR was detected in 169 eyes (3.4%) of 138 subjects (5.4%), and PMF was detected in 106 eyes (2.1%) of 97 subjects (3.8%). By univariate analyses, compared with ERM− eyes or subjects, higher Scheie’s H grade (p < 0.0001), S grade (p < 0.0001), and glaucoma prevalence (p = 0.0440) were found in ERM+ eyes, and older age (p < 0.0001), more frequent histories of hypertension (p = 0.0033) and hyperlipidemia (p = 0.0441), and more frequent uses of medication for hypertension (p = 0.0034) and hyperlipidemia (p = 0.0074), shorter body height (p = 0.0122), and higher systolic blood pressure (p = 0.0078), and thicker intimal medial thickness (p = 0.0318) were found in ERM+ subjects. By multivariate analyses, older age (p < 0.0001, estimate = 0.05/year) was the only significant factor of ERM prevalence. Age-standardized prevalence of ERM was calculated to be 2.4%, 6.7%, and 13.3% for all ages, subjects older than 40 years, and subjects older than 65 years, respectively. We reported the prevalence of ERM and its subclasses in Japanese subjects. Since its prevalence is remarkably high in older subjects, an ERM can be seen as an important cause of visual impairment in Japan and in areas of the world where individuals live to an advanced age.
Cryptococcal Immune Reconstitution Inflammatory Syndrome: From Blood and Cerebrospinal Fluid Biomarkers to Treatment Approaches
Authors: Brienze Vânia Maria Sabadoto , André Júlio César , Liso Elisabete , Vlasova-St. Louis Irina
Published: January 2021

Immune reconstitution inflammatory syndrome (IRIS) presents as an exaggerated immune reaction that occurs during dysregulated immune restoration in immunocompromised patients in late-stage human immunodeficiency virus (HIV) infection who have commenced antiretroviral treatments (ART). Virtually any opportunistic pathogen can provoke this type of immune restoration disorder. In this review, we focus on recent developments in the identification of risk factors for Cryptococcal IRIS and on advancements in our understanding of C-IRIS immunopathogenesis. We overview new findings in blood and cerebrospinal fluid which can potentially be useful in the prediction and diagnosis of cryptococcal meningitis IRIS (CM-IRIS). We assess current therapeutic regimens and novel treatment approaches to combat CM-IRIS. We discuss the utility of biomarkers for clinical monitoring and adjusting treatment modalities in acquired immunodeficiency syndrome (AIDS) patients co-infected with Cryptococcus who have initiated ART.
Towards a Notion of Basis for Knowledge-Based Systems—Applications
Authors: Aranda-Corral Gonzalo A. , Borrego-Díaz Joaquín , Galán-Páez Juan , Rodríguez-Chavarría Daniel
Published: January 2021

In the paradigm of Knowledge-Based Systems (KBS), the design of methods to simplify the reasoning leads to more efficient processes. A point of view that provides valuable insights is the algebraic one. In this work, a notion of basis (and dimension) for Knowledge Bases in Propositional Logic associated with knowledge forgetting is introduced. It is based on ideas that come from the translation of such logic in (Computer) Algebra, particularly from the interpretation of variable forgetting. In this paper, the concept of weak base is defined as a set of variables sufficient to decide the consistency using variable forgetting. Several applications of weak bases are presented in order to show their usefulness in KBS reasoning and to justify their study and use in solving problems within this topic.
Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer
Authors: Gargouch Nesrine , Elleuch Fatma , Karkouch Ines , Tabbene Olfa , Pichon Chantal , Gardarin Christine , Rihouey Christophe , Picton Luc , Abdelkafi Slim , Fendri Imen , Laroche Céline
Published: January 2021

Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs. However, lot of them remain to be discovered and tested. In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells. Results have shown that all EPS samples present some biological activity. For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain. For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration. Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%. Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.
Efficacy of Lesion Specific Portals in Endoscopic Treatment of Calcaneal Bone Cyst: A Case Report and Literature Review
Authors: Yi Young , Lee Jeong Seok , Kim Jahyung , Jin So Young , Won Sung Hun , Cho Jaeho , Chun Dong-Il
Published: January 2021

Background: Calcaneal bone cysts rarely occur and most of them are known to be benign. Among them, simple bone cysts (SBCs) third most commonly occur in the calcaneus and of the many surgical treatment options, endoscopic curettage is recently gaining popularity among surgeons due to its advantages of minimal invasiveness and optimal visualization. As for portal placement for endoscopy, two lateral portals are considered a standard technique, but no rationale has been established for SBCs with abnormal geometry. This case report suggests an SBC with secondary aneurysmal change located outside the Ward’s triangle, as well as an appropriate endoscopic approach. Case Presentation: An 18-year-old male high school student presented with a main complaint of pain at the hind foot level for the past one year, without significant improvement from conservative treatment. An endoscopic curettage through the lesion specific two posterior portals and bone graft using allogeneic cancellous bone were performed. SBC with a secondary aneurysmal bone cyst was diagnosed on pathology. At a one-year follow-up, the patient was painless and had returned to his regular activities. Physical and radiographic examinations revealed that the lesion was completely healed without any evidence of recurrence. Conclusion: For calcaneal bone cysts located at the posterior aspect of the calcaneus, eccentrically medial and abnormally long anterior-posteriorly, we suggest an endoscopic procedure using lesion specific portals such as two posterior portals.
Yeast Cell Cake Characterization in Alcohol Solution for Efficient Microfiltration
Authors: Katagiri Nobuyuki , Tomimatsu Keisuke , Date Keiichi , Iritani Eiji
Published: January 2021

Microfiltration is widely used to remove microbial cells from the fermentation broth in the downstream processing of biotechnological products. Because filtration behaviors are strongly affected by the characteristics of the microbial cell cake formed on the surface of the membrane, insights into the cake structure facilitate the design and operation of filter equipment and membranes. In the alcohol fermentation process using a yeast strain, the cake characteristics are considered to be complicated because yeast cells are strongly influenced by external factors such as filtration pressure and alcohol concentration. In this study, we evaluated the membrane filtration properties, in particular the cake characteristics of a yeast suspension containing alcohol. Microfiltration experiments were performed in the dead-end filtration mode using yeast suspensions with several ethanol concentrations (0–20 wt%) under constant pressure. Flux decline behaviors caused by yeast cake were put in a similar form for 0–15 wt% ethanol concentrations. In contrast, a severe flux decline was observed for the suspension with 20 wt% ethanol concentration. It was also observed that in the membrane filtration of yeast cells with 20 wt% ethanol concentration, the cake structure became denser and the filtration resistance remarkably increased because of cellular destruction. Furthermore, the yeast cake exhibited a high compressibility in the solution containing a 20 wt% ethanol concentration. Therefore, the filtration rate of the alcoholic fermentation broth is not significantly improved by increased pressure due to the increase in the cake resistance.
Glucagon-Like Peptide-1 Receptor Agonists for Treatment of Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis: An Updated Meta-Analysis of Randomized Controlled Trials
Authors: Mantovani Alessandro , Petracca Graziana , Beatrice Giorgia , Csermely Alessandro , Lonardo Amedeo , Targher Giovanni
Published: January 2021

To assess the efficacy of glucagon-like peptide-1 receptor agonists (GLP-1 RAs) for treatment of nonalcoholic fatty liver disease (NAFLD) or steatohepatitis (NASH), we performed a systematic review and meta-analysis of randomized controlled trials (RCTs). Three large electronic databases were systematically searched (up to 15 December 2020) to identify placebo-controlled or active-controlled RCTs using different GLP-1 RAs. We included eleven placebo-controlled or active-controlled phase-2 RCTs (involving a total of 936 middle-aged individuals) that used liraglutide (n = 6 RCTs), exenatide (n = 3 RCTs), dulaglutide (n = 1 RCT) or semaglutide (n = 1 RCT) to specifically treat NAFLD or NASH, detected by liver biopsy (n = 2 RCTs) or imaging techniques (n = 9 RCTs). Compared to placebo or reference therapy, treatment with GLP-1 RAs for a median of 26 weeks was associated with significant reductions in the absolute percentage of liver fat content on magnetic resonance-based techniques (pooled weighted mean difference: −3.92%, 95% confidence intervals (CI) −6.27% to −1.56%) and serum liver enzyme levels, as well as with greater histological resolution of NASH without worsening of liver fibrosis (pooled random-effects odds ratio 4.06, 95% CI 2.52–6.55; for liraglutide and semaglutide only). In conclusion, treatment with GLP-1 RAs (mostly liraglutide and semaglutide) is a promising treatment option for NAFLD or NASH that warrants further investigation.
Moxifloxacin Activates the SOS Response in Mycobacterium tuberculosis in a Dose- and Time-Dependent Manner
Authors: Iacobino Angelo , Piccaro Giovanni , Pardini Manuela , Fattorini Lanfranco , Giannoni Federico
Published: January 2021

Previous studies on Escherichia coli demonstrated that sub-minimum inhibitory concentration (MIC) of fluoroquinolones induced the SOS response, increasing drug tolerance. We characterized the transcriptional response to moxifloxacin in Mycobacterium tuberculosis. Reference strain H37Rv was treated with moxifloxacin and gene expression studied by qRT-PCR. Five SOS regulon genes, recA, lexA, dnaE2, Rv3074 and Rv3776, were induced in a dose- and time-dependent manner. A range of moxifloxacin concentrations induced recA, with a peak observed at 2 × MIC (0.25 μg/mL) after 16 h. Another seven SOS responses and three DNA repair genes were significantly induced by moxifloxacin. Induction of recA by moxifloxacin was higher in log-phase than in early- and stationary-phase cells, and absent in dormant bacilli. Furthermore, in an H37Rv fluoroquinolone-resistant mutant carrying the D94G mutation in the gyrA gene, the SOS response was induced at drug concentrations higher than the mutant MIC value. The 2 × MIC of moxifloxacin determined no significant changes in gene expression in a panel of 32 genes, except for up-regulation of the relK toxin and of Rv3290c and Rv2517c, two persistence-related genes. Overall, our data show that activation of the SOS response by moxifloxacin, a likely link to increased mutation rate and persister formation, is time, dose, physiological state and, possibly, MIC dependent.
Bacteriophages as a Potential 360-Degree Pathogen Control Strategy
Authors: D’Accolti Maria , Soffritti Irene , Mazzacane Sante , Caselli Elisabetta
Published: January 2021

Bacteriophages are viruses that exclusively kill bacteria and are the most ubiquitous organisms on the planet. Since their discovery, bacteriophages have been considered an important weapon to fight human and animal infections of bacterial origin due to their specific ability to attack the associated target bacteria. With the discovery of antibiotics, phage treatment was progressively abandoned in Western countries. However, due to the recent emergence of growing antimicrobial resistance (AMR) to antibiotics, interest in phage use in human therapy has once again grown. Similarly, at the environmental level, the extensive use of disinfectants based on chemicals, including biocides in agriculture, has been associated with the emergence of resistance against disinfectants themselves, besides having a high environmental impact. Due to these issues, the applications of phages with biocontrol purposes have become an interesting option in several fields, including farms, food industry, agriculture, aquaculture and wastewater plants. Notably, phage action is maintained even when the target bacteria are multidrug resistant (MDR), rendering this option extremely interesting in counteracting AMR emergence both for therapeutical and decontamination purposes. Based on this, bacteriophages have been interestingly proposed as environmental routine sanitizers in hospitals, to counteract the spread of the pathogenic MDR bacteria that persistently contaminate hard surfaces. This review summarizes the studies aimed at evaluating the potential use of phages as decontaminants, with a special focus on hospital sanitation.
Rare-Metal (In, Bi, Te, Se, Be) Mineralization of Skarn Ores in the Pitkäranta Mining District, Ladoga Karelia, Russia
Authors: Ivashchenko Vasily I.
Published: January 2021

The results of the study of rare-metal (Bi, Te, Se. Be, In) mineralization of skarn deposits (Sn, Zn) in the Pitkäranta Mining District, genetically related to the Salmi anorthosite-rapakivi granite batholiths of Early Riphean age are reported. Minerals and their chemical composition were identified on the base of optical microscopy as well as electron microanalysis. The diversity of rare-metal ore mineralization (native metals, oxides, and hydroxides, carbonates, tellurides, selenides, sulfides, sulphosalts, borates, and silicates) in Pitkäranta Mining District ores is indicative of considerable variations in the physicochemical conditions of their formation controlled by the discrete-pulse-like supply of fluids. Bismuth, wittichenite, and matildite are the most common rare-metal minerals. Sulfosalts of the bismuthinite-aikinite series are represented only by its end-members. The absence of solid solution exsolution structures in sulfobismuthides suggests that they crystallized from hydrothermal solutions at low temperatures. Be (>10 minerals) and In (roquesite) minerals occur mainly in aposkarn greisens. Roquesite in Pitkäranta Mining District ores formed upon greisen alteration of skarns with In released upon the alteration of In-bearing solid sphalerite (Cu1+ In3+) ↔ (Zn2+, Fe2+) and chalcopyrite In3+ ↔ Fe3+ and 2Fe3+ ↔ (Fe2+, Zn2+) Sn4+ solutions. Sphalerite with an average In concentration of 2001 ppm, is a major In-bearing mineral in the ores.
Compositional Variability of Monazite–Cheralite–Huttonite Solid Solutions, Xenotime, and Uraninite in Geochemically Distinct Granites with Special Emphasis to the Strongly Fractionated Peraluminous Li–F–P-Rich Podlesí Granite System (Erzgebirge/Krušné Hory Mts., Central Europe)
Authors: Breiter Karel , Förster Hans-Jürgen
Published: January 2021

A comprehensive study of monazite–cheralite–huttonite solid solutions (s.s.) and xenotime from the highly evolved, strongly peraluminous P–F–Li-rich Podlesí granite stock in the Krušné Hory Mts., Czech Republic, indicates that, with the increasing degree of magmatic and high-T early post-magmatic evolution, the content of the cheralite component in monazite increases and the relative dominance of middle rare earth elements (MREE) in xenotime becomes larger. Considering the overall compositional signatures of these two accessory minerals in the late Variscan granites of the Erzgebirge/Krušné Hory Mts., three types of granites can be distinguished: (i) chemically less evolved F-poor S(I)- and A-type granites contain monazite with a smooth, mostly symmetric chondrite-normalized (CN) rare-earth elements (REE) pattern gradually declining from La to Gd; associated xenotime is Y-rich (˃0.8 apfu Y) with a flat MREE–HREE (heavy rare earth elements) pattern; (ii) fractionated A-type granites typically contain La-depleted monazite with Th accommodated as the huttonite component, combined with usually Y-poor (0.4–0.6 apfu Y) xenotime characterized by a smoothly inclining, Yb–Lu-dominant CN-REE pattern; (iii) fractionated peraluminous Li-mica granites host monazite with a flat, asymmetric (kinked at La and Nd) CN-LREE pattern, with associated xenotime distinctly MREE (Gd–Tb–Dy)-dominant. Monazite and xenotime account for the bulk of the REE budgets in all types of granite. In peraluminous S(I)-type granites, which do not bear thorite, almost all Th is accommodated in monazite–cheralite s.s. In contrast, Th budgets in A-type granites are accounted for by monazite–huttonite s.s. together with thorite. The largest portion of U is accommodated in uraninite, if present.
Design and Synthesis of π-Extended Resveratrol Analogues and In Vitro Antioxidant and Anti-Inflammatory Activity Evaluation
Authors: Nam Ki Yoon , Damodar Kongara , Lee Yeontaek , Park Lee Seul , Gim Ji Geun , Park Jae Phil , Jeon Seong Ho , Lee Jeong Tae
Published: January 2021

The research on resveratrol (1) has been conducted intensively over a long time due to its proven antioxidant activity and disease-fighting capabilities. Many efforts have also been made to increase these biological effects. In the present study, six new extended aromatic resveratrol analogues containing naphthalene (2) and its bioisosteres quinoline (3 and 4), isoquinoline (5) quinoxaline (6) and quinazoline (7) scaffolds were designed and synthesized using an annulation strategy. The antioxidant and anti-inflammatory activities of these compounds were investigated. All compounds showed better antioxidant activity than resveratrol in ABTS assay. As for the anti-inflammatory test, 5 and 7 exhibited better activity than resveratrol. It is worth noting that nitrogen substitution on the extended aromatic resveratrol analogues has a significant impact on cell viability. Taking the antioxidant activities and NO inhibition activities into consideration, we conclude that isoquinoline analogue 5 may qualify for the further investigation of antioxidant and anti-inflammatory therapy. Furthermore, our study results suggest that in order to improve the biological activity of polyphenolic compounds, extended aromaticity and nitrogen substitution strategy could be a viable method for the design of future drug candidates.
Augmentation of the Female Reproductive System Using Honey: A Mini Systematic Review
Authors: Ismail Nur Hilwani , Ibrahim Siti Fatimah , Jaffar Farah Hanan Fathihah , Mokhtar Mohd Helmy , Chin Kok Yong , Osman Khairul
Published: January 2021

Phytochemical contents of honey are presumed to be beneficial to the female reproductive system (FRS). However, the biological effects of honey supplementation (HS) in vivo on the FRS remain unclear. This review aims to investigate the current literature on the effects of HS on the FRS, particularly on the sex hormone profile and reproductive organs (uterus and vagina). A systematic literature search using Scopus, MEDLINE via Ovid and Cochrane Library databases was conducted. Records were screened and identified for preclinical and clinical studies addressing the effects of HS on the FRS. Data on populations, interventions, outcomes and methodological quality were extracted. Studies were synthesised using tables and written summaries. Of the 198 identified records, six fulfilled the inclusion criteria. All six records were used for data extraction: two experimental studies using rats as the model organism and four human clinical studies of honey on female reproductive health. HS elevated the progesterone levels, restrained body weight increase, prevented uterine and vaginal atrophies in ovariectomised rats, attenuated symptoms of candidiasis and improved oxidative status in patients. Current evidence shows that short-term HS following surgical or physiological menopause exerts an oestrogenic, antioxidant and anti-inflammatory effect on the FRS. However, insufficient long-term studies preclude any definitive conclusions.
Druggable Transient Pockets in Protein Kinases
Authors: Umezawa Koji , Kii Isao
Published: January 2021

Drug discovery using small molecule inhibitors is reaching a stalemate due to low selectivity, adverse off-target effects and inevitable failures in clinical trials. Conventional chemical screening methods may miss potent small molecules because of their use of simple but outdated kits composed of recombinant enzyme proteins. Non-canonical inhibitors targeting a hidden pocket in a protein have received considerable research attention. Kii and colleagues identified an inhibitor targeting a transient pocket in the kinase DYRK1A during its folding process and termed it FINDY. FINDY exhibits a unique inhibitory profile; that is, FINDY does not inhibit the fully folded form of DYRK1A, indicating that the FINDY-binding pocket is hidden in the folded form. This intriguing pocket opens during the folding process and then closes upon completion of folding. In this review, we discuss previously established kinase inhibitors and their inhibitory mechanisms in comparison with FINDY. We also compare the inhibitory mechanisms with the growing concept of “cryptic inhibitor-binding sites.” These sites are buried on the inhibitor-unbound surface but become apparent when the inhibitor is bound. In addition, an alternative method based on cell-free protein synthesis of protein kinases may allow the discovery of small molecules that occupy these mysterious binding sites. Transitional folding intermediates would become alternative targets in drug discovery, enabling the efficient development of potent kinase inhibitors.
Induction of Cytokines by Nucleic Acid Nanoparticles (NANPs) Depends on the Type of Delivery Carrier
Authors: Avila Yelixza I. , Chandler Morgan , Cedrone Edward , Newton Hannah S. , Richardson Melina , Xu Jie , Clogston Jeffrey D. , Liptrott Neill J. , Afonin Kirill A. , Dobrovolskaia Marina A.
Published: January 2021

Recent insights into the immunostimulatory properties of nucleic acid nanoparticles (NANPs) have demonstrated that variations in the shape, size, and composition lead to distinct patterns in their immunostimulatory properties. While most of these studies have used a single lipid-based carrier to allow for NANPs’ intracellular delivery, it is now apparent that the platform for delivery, which has historically been a hurdle for therapeutic nucleic acids, is an additional means to tailoring NANP immunorecognition. Here, the use of dendrimers for the delivery of NANPs is compared to the lipid-based platform and the differences in resulting cytokine induction are presented.
Kinetics Growth and Recovery of Valuable Nutrients from Selangor Peat Swamp and Pristine Forest Soils Using Different Extraction Methods as Potential Microalgae Growth Enhancers
Authors: Yaacob Nor Suhaila , Ahmad Mohd Fadzli , Kawasaki Nobuyuki , Maniyam Maegala Nallapan , Abdullah Hasdianty , Hashim Emi Fazlina , Sjahrir Fridelina , Wan Mohd Zamri Wan Muhammad Ikram , Komatsu Kazuhiro , Kuwahara Victor S.
Published: January 2021

Soil extracts are useful nutrients to enhance the growth of microalgae. Therefore, the present study attempts for the use of virgin soils from Peninsular Malaysia as growth enhancer. Soils collected from Raja Musa Forest Reserve (RMFR) and Ayer Hitam Forest Reserve (AHFR) were treated using different extraction methods. The total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and dissolved organic carbon (DOC) concentrations in the autoclave methods were relatively higher than natural extraction with up to 132.0 mg N/L, 10.7 mg P/L, and 2629 mg C/L, respectively for RMFR. The results of TDN, TDP, and DOC suggested that the best extraction methods are autoclaved at 121 °C twice with increasing 87%, 84%, and 95%, respectively. Chlorella vulgaris TRG 4C dominated the growth at 121 °C twice extraction method in the RMRF and AHRF samples, with increasing 54.3% and 14%, respectively. The specific growth rate (µ) of both microalgae were relatively higher, 0.23 d−1 in the Ayer Hitam Soil. This extract served well as a microalgal growth promoter, reducing the cost and the needs for synthetic medium. Mass production of microalgae as aquatic feed will be attempted eventually. The high recovery rate of nutrients has a huge potential to serve as a growth promoter for microalgae.
Prenylated Trans-Cinnamic Esters and Ethers against Clinical Fusarium spp.: Repositioning of Natural Compounds in Antimicrobial Discovery
Authors: Oufensou Safa , Casalini Stefano , Balmas Virgilio , Carta Paola , Chtioui Wiem , Dettori Maria A. , Fabbri Davide , Migheli Quirico , Delogu Giovanna
Published: January 2021

Onychomycosis is a common nail infection mainly caused by species belonging to the F. oxysporum, F. solani, and F. fujikuroi species complexes. The aim of this study was to evaluate the in vitro susceptibility of six representative strains of clinically relevant Fusarium spp. toward a set of natural-occurring hydroxycinnamic acids and their derivatives with the purpose to develop naturally occurring products in order to cope with emerging resistance phenomena. By introducing a prenylated chain at one of the hydroxy groups of trans-cinnamic acids 1–3, ten prenylated derivatives (coded 4–13) were preliminarily investigated in solid Fusarium minimal medium (FMM). Minimal inhibitory concentration (MIC) and lethal dose 50 (LD50) values were then determined in liquid FMM for the most active selected antifungal p-coumaric acid 3,3′-dimethyl allyl ester 13, in comparison with the conventional fungicides terbinafine (TRB) and amphotericin B (AmB), through the quantification of the fungal growth. Significant growth inhibition was observed for prenylated derivatives 4–13, evidencing ester 13 as the most active. This compound presented MIC and LD50 values (62–250 µM and 7.8–125 µM, respectively) comparable to those determined for TRB and AmB in the majority of the tested pathogenic strains. The position and size of the prenylated chain and the presence of a free phenol OH group appear crucial for the antifungal activity. This work represents the first report on the activity of prenylated cinnamic esters and ethers against clinical Fusarium spp. and opens new avenues in the development of alternative antifungal compounds based on a drug repositioning strategy.
Efficient Adsorption of Methylene Blue by Porous Biochar Derived from Soybean Dreg Using a One-Pot Synthesis Method
Authors: Ying Zhiwei , Chen Xinwei , Li He , Liu Xinqi , Zhang Chi , Zhang Jian , Yi Guofu
Published: January 2021

Soybean dreg is a by-product of soybean products production, with a large consumption in China. Low utilization value leads to random discarding, which is one of the important sources of urban pollution. In this work, porous biochar was synthesized using a one-pot method and potassium bicarbonate (KHCO3) with low-cost soybean dreg (SD) powder as the carbon precursor to investigating the adsorption of methylene blue (MB). The prepared samples were characterized with scanning electron microscopy (SEM), transmission electron microscopy (TEM), elemental analyzer (EA), Brunauer-Emmett-Teller (BET), X-ray diffractometer (XRD), Raman spectroscopy (Raman), Fourier transform infrared spectrometer (FTIR), and X-ray photoelectron spectroscopy (XPS). The obtained SDB-K-3 showed a high specific surface area of 1620 m2 g−1, a large pore volume of 0.7509 cm3 g−1, and an average pore diameter of 1.859 nm. The results indicated that the maximum adsorption capacity of SDB-K-3 to MB could reach 1273.51 mg g−1 at 318 K. The kinetic data were most consistent with the pseudo-second-order model and the adsorption behavior was more suitable for the Langmuir isotherm equation. This study demonstrated that the porous biochar adsorbent can be prepared from soybean dreg by high value utilization, and it could hold significant potential for dye wastewater treatment in the future.
Interparticle Spacing Effect among Quantum Dots with High-Pressure Regulation
Authors: Cheng Ji-Chao , Pan Ling-Yun , Huang Xiao-Li , Huang Yan-Ping , Wang Ying-Hui , Xu Shu-Ping , Li Fang-Fei , Men Zhi-Wei , Cui Tian
Published: January 2021

In this paper, we explore whether interparticle spacing affects steady-state and transient-state optical properties by comparing close-packed CdSe/ZnS–quantum dots (QDs) and CdSe/ZnS–QDs dispersed in polymethyl methacrylate (PMMA). High–pressure is an effective physical means to adjust the interparticle spacing of QDs, which may artificially expand the application of QDs further. The results under high–pressure indicate that it is the reduced interparticle spacing rather than the enhanced quantum confinement effect with volume compression that has a stronger effect on exciton relaxation of CdSe/ZnS–QDs. This work is hoped to help us further understand the effect of interparticle spacing among QDs in various integrated environments.
Room Temperature Processed Double Electron Transport Layers for Efficient Perovskite Solar Cells
Authors: Huang Wen , Zhang Rui , Xia Xuwen , Steichen Parker , Liu Nanjing , Yang Jianping , Chu Liang , Li Xing’ao
Published: January 2021

Zinc Oxide (ZnO) has been regarded as a promising electron transport layer (ETL) in perovskite solar cells (PSCs) owing to its high electron mobility. However, the acid-nonresistance of ZnO could destroy organic-inorganic hybrid halide perovskite such as methylammonium lead triiodide (MAPbI3) in PSCs, resulting in poor power conversion efficiency (PCE). It is demonstrated in this work that Nb2O5/ZnO films were deposited at room temperature with RF magnetron sputtering and were successfully used as double electron transport layers (DETL) in PSCs due to the energy band matching between Nb2O5 and MAPbI3 as well as ZnO. In addition, the insertion of Nb2O5 between ZnO and MAPbI3 facilitated the stability of the perovskite film. A systematic investigation of the ZnO deposition time on the PCE has been carried out. A deposition time of five minutes achieved a ZnO layer in the PSCs with the highest power conversion efficiency of up to 13.8%. This excellent photovoltaic property was caused by the excellent light absorption property of the high-quality perovskite film and a fast electron extraction at the perovskite/DETL interface.
Fe3O4@C Nanoparticles Synthesized by In Situ Solid-Phase Method for Removal of Methylene Blue
Authors: Xiang Hengli , Ren Genkuan , Zhong Yanjun , Xu Dehua , Zhang Zhiye , Wang Xinlong , Yang Xiushan
Published: January 2021

Fe3O4@C nanoparticles were prepared by an in situ, solid-phase reaction, without any precursor, using FeSO4, FeS2, and PVP K30 as raw materials. The nanoparticles were utilized to decolorize high concentrations methylene blue (MB). The results indicated that the maximum adsorption capacity of the Fe3O4@C nanoparticles was 18.52 mg/g, and that the adsorption process was exothermic. Additionally, by employing H2O2 as the initiator of a Fenton-like reaction, the removal efficiency of 100 mg/L MB reached ~99% with Fe3O4@C nanoparticles, while that of MB was only ~34% using pure Fe3O4 nanoparticles. The mechanism of H2O2 activated on the Fe3O4@C nanoparticles and the possible degradation pathways of MB are discussed. The Fe3O4@C nanoparticles retained high catalytic activity after five usage cycles. This work describes a facile method for producing Fe3O4@C nanoparticles with excellent catalytic reactivity, and therefore, represents a promising approach for the industrial production of Fe3O4@C nanoparticles for the treatment of high concentrations of dyes in wastewater.
Ultracentrifugation Techniques for the Ordering of Nanoparticles
Authors: Xu Xufeng , Cölfen Helmut
Published: January 2021

A centrifugal field can provide an external force for the ordering of nanoparticles. Especially with the knowledge from in-situ characterization by analytical (ultra)centrifugation, nanoparticle ordering can be rationally realized in preparative (ultra)centrifugation. This review summarizes the work back to the 1990s, where intuitive use of centrifugation was achieved for the fabrication of colloidal crystals to the very recent work where analytical (ultra)centrifugation is employed to tailor-make concentration gradients for advanced materials. This review is divided into three main parts. In the introduction part, the history of ordering microbeads in gravity is discussed and with the size of particles reduced to nanometers, a centrifugal field is necessary. In the next part, the research on the ordering of nanoparticles in analytical and preparative centrifugation in recent decades is described. In the last part, the applications of the functional materials, fabricated from centrifugation-induced nanoparticle superstructures are briefly discussed.
Nursing Education in a Real-Life Context: The Teaching Ward Round
Authors: Martínez-Galiano Juan Miguel , Parra-Anguita Laura , Delgado-Rodríguez Miguel , González-Cabrera Manuel
Published: January 2021

Education in nursing is continually changing. The didactic methods used in other fields may be useful for closing the gap between theoretical learning and the reality of practical nursing. This study aimed to determine the association between a teaching model centered on the reality of nursing care, which is individualized to each context, and knowledge acquisition. A controlled experimental study was conducted with random allocation to two groups of students in their second year of a nursing degree (University of Jaén). The control group undertook practical work placements according to the traditional model. The intervention group participated in a “teaching round” during their practical placements. Knowledge tests were conducted after the placements. No significant differences were found for age or education level between the students of the control group (n = 46) and the intervention group (n = 48). In terms of the association between participation in the teaching round and the knowledge test (maximum score of 10), the mean grade in the intervention group was 8.83 ± 0.22, while it was 7.68 ± 0.23 in the control group (p = 0.001). The teaching round increased the student’s acquisition of knowledge, even though this was not reflected in the global grade of the course.
Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays
Authors: Bonelli Piero , Dei Giudici Silvia , Peruzzu Angela , Mura Lorena , Santucciu Cinzia , Maestrale Caterina , Masala Giovanna
Published: January 2021

Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis in animals and humans. Different E. granulosuss.l. genotypes exhibit great diversity in their life cycle, host selectivity and pathogenicity. For this reason, the study of genetic variation within Echinococcus species is of importance for their epidemiological implication. We employed two SNP genotyping technologies to distinguish G1 and G3 E. granulosus sensu stricto (s.s.). genotypes. The genotypes of DNA samples (n = 28) extracted from hydatid cysts of different animal species were identified by amplification and sequencing of a fragment of the mitochondrial nad5 gene. Two SYBR green and three TaqMan real time PCR assays were developed for targeting of three nad5 informative positions (SNP758, 1123, and 1380) known to be able to discriminate G1 from G3. Genotyping by SYBR Green PCR based on cycle threshold (Ct) with melting temperature (Tm) analysis and performed on SNP1123 and SNP1380 failed to identify one DNA sample. TaqMan assays for SNP758, 1123 and 1380 effectively confirmed genotype identification obtained by Sanger sequencing. Our results demonstrated that the combination of the three Taqman assays developed in this study represents a valuable and cost effective tool alternative to DNA sequencing for E. granulosus s.s. genotyping.
Acinetobacter baumannii: Its Clinical Significance in Human and Veterinary Medicine
Authors: Nocera Francesca Paola , Attili Anna-Rita , De Martino Luisa
Published: January 2021

Acinetobacter baumannii is a Gram-negative, opportunistic pathogen, causing severe infections difficult to treat. The A. baumannii infection rate has increased year by year in human medicine and it is also considered as a major cause of nosocomial infections worldwide. This bacterium, also well known for its ability to form biofilms, has a strong environmental adaptability and the characteristics of multi-drug resistance. Indeed, strains showing fully resistant profiles represent a worrisome problem in clinical therapeutic treatment. Furthermore, A. baumannii-associated veterinary nosocomial infections has been reported in recent literature. Particularly, carbapenem-resistant A. baumannii can be considered an emerging opportunistic pathogen in human medicine as well as in veterinary medicine.
Antibiotic Resistance, Biofilm Formation, and Presence of Genes Encoding Virulence Factors in Strains Isolated from the Pharmaceutical Production Environment
Authors: Ratajczak Magdalena , Kaminska Dorota , Dlugaszewska Jolanta , Gajecka Marzena
Published: January 2021

The spread of bacterial resistance to antibiotics affects various areas of life. The aim of this study was to assess the occurrence of Pseudomonas aeruginosa, and other bacteria mainly from orders Enterobacterales and Staphylococcus in the pharmaceutical production sites, and to characterize isolated strains in the aspects of antibiotic resistance, biofilm formation, and presence of genes encoding virulence factors. Genes encoding selected virulence factors were detected using PCR techniques. Antimicrobial susceptibility testing was applied in accordance with the EUCAST recommendations. A total of 46 P. aeruginosa strains were isolated and 85% strains showed a strong biofilm-forming ability. The qualitative identification of genes taking part in Quorum Sensing system demonstrated that over 89% of strains contained lasR and rhlI genes. An antimicrobial susceptibility testing revealed nine strains resistant to at least one antibiotic, and two isolates were the metallo-β-lactamase producers. Moreover, the majority of P. aeruginosa strains contained genes encoding various virulence factors. Presence of even low level of pathogenic microorganisms or higher level of opportunistic pathogens and their toxic metabolites might result in the production inefficiency. Therefore, the prevention of microbial contamination, effectiveness of sanitary and hygienic applied protocols, and constant microbiological monitoring of the environment are of great importance.
Acknowledgment to Reviewers of Pharmacy in 2020
Authors:   Pharmacy Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Pharmacy maintains its standards for the high quality of its published papers [...]
Single Headlamp with Low- and High-Beam Light
Authors: Ying Shang-Ping , Chen Bing-Mau , Fu Han-Kuei , Yeh Chen-Yu
Published: January 2021

Generally, automobiles are typically equipped with separate headlamp lanterns for generating low- and high-beam light. Compared with separate headlamp lanterns, a single headlamp producing both low- and high-beam light can be more compact and have less mechanical complexity. The single headlamp structure has become a main emphasis of research that manufacturers will continue to focus great efforts on in the future. A novel design of a single headlamp generating both low- and high-beam light is proposed in this study. The proposed headlamp consists of a compound ellipsoidal reflector, a baffle plate, a condenser lens, and LED array devices generating low- and high-beam light. The compound ellipsoidal reflector comprises a primary ellipsoidal reflector for generating low-beam light and a secondary ellipsoidal reflector for generating high-beam light. Monte Carlo ray tracing simulations were performed to confirm the optical characteristics of the proposed design. A prototype of the proposed headlamp was also fabricated and assembled to verify the design’s effectiveness. The simulated and measured illuminance distributions of the low-beam and high-beam light had the desired light patterns. Moreover, all the simulated and measured illuminances of each point and line met the ECE R112 regulation for low-beam and high-beam light. The proposed headlamp in this study is feasible for the application of single headlamp generating both low- and high-beam light.
First Restoration Experiment for Gongolaria barbata in Slovenian Coastal Waters. What Can Go Wrong?
Authors: Orlando-Bonaca Martina , Pitacco Valentina , Slavinec Petra , Šiško Milijan , Makovec Tihomir , Falace Annalisa
Published: January 2021

The global decline of brown algal forests along rocky coasts is causing an exceptional biodiversity loss. Regardless of conservation efforts, different techniques have been developed for large-scale restoration strategies in the Mediterranean Sea. In this study we tested ex situ pilot restoration of Gongolaria barbata (=Treptacantha barbata) for the first time in Slovenian coastal waters. Healthy apical fronds of the species were collected and the development of recruits on clay tiles was followed under laboratory conditions for 20 days. Despite the experimental difficulties experienced, especially due to the lack of antibiotics to prevent the growth of the biofilm, G. barbata recruits were outplanted in the sea on two concrete plates with 48 tiles each, protected by purpose-built cages to avoid grazing by herbivorous fish. The high survival rate of juveniles after four months in the field (89% of the tiles on the plate that was constantly protected) suggests that outplanting G. barbata is an operable approach for restoration efforts in the northern Adriatic Sea. Our first experiment in Slovenian coastal waters provides new information for the optimization of the best practices during the laboratory cultivation and addresses the early steps of restoration and introduction of young thalli in the natural environment.
The Ubiquitin Switch in Plant Stress Response
Authors: Doroodian Paymon , Hua Zhihua
Published: January 2021

Ubiquitin is a 76 amino acid polypeptide common to all eukaryotic organisms. It functions as a post-translationally modifying mark covalently linked to a large cohort of yet poorly defined protein substrates. The resulting ubiquitylated proteins can rapidly change their activities, cellular localization, or turnover through the 26S proteasome if they are no longer needed or are abnormal. Such a selective modification is essential to many signal transduction pathways particularly in those related to stress responses by rapidly enhancing or quenching output. Hence, this modification system, the so-called ubiquitin-26S proteasome system (UPS), has caught the attention in the plant research community over the last two decades for its roles in plant abiotic and biotic stress responses. Through direct or indirect mediation of plant hormones, the UPS selectively degrades key components in stress signaling to either negatively or positively regulate plant response to a given stimulus. As a result, a tightly regulated signaling network has become of much interest over the years. The ever-increasing changes of the global climate require both the development of new crops to cope with rapid changing environment and new knowledge to survey the dynamics of ecosystem. This review examines how the ubiquitin can switch and tune plant stress response and poses potential avenues to further explore this system.
Centrality of Buddhist Religiosity Scale: Adaptation and Validation of the Centrality of Religiosity Scale in a Buddhist Sample in Vietnam
Authors: Nguyen Hang Thi Minh , Ackert Michael , Flückiger Christoph , Scheiblich Herbert
Published: January 2021

This paper describes an adaptation of the Centrality of Religiosity Scale to the Buddhist religious tradition (CBRS) and a validation in Vietnam. The sample included data from 421 Vietnamese Buddhists (300 females, 121 males), aged 17 to 71 years (M=35.03, SD=13.09). The results provided evidence for good psychometric properties of the short, intermediate, and long version: CBRS-5, CBRS-10, and CBRS-15 respectively. Specifically, exploratory and confirmatory factor analyses supported the measure’s original five-factor structure: intellect, ideology, public practice, private practice, and religious experience. Furthermore, the Centrality of Buddhist Religiosity has proven to be a stable psychological construct across the three versions of CBRS and was associated strongly with the Gordon Allport’s notion of the intrinsic religious orientation. The results also suggested that the Stefan Huber’s centrality of religiosity model can capture the Buddhist religiosity and that the CBRS can be used to measure the five theoretical defined core dimensions of religiosity in Buddhism.
A Predictive Vehicle Ride Sharing Recommendation System for Smart Cities Commuting
Authors: Anagnostopoulos Theodoros
Published: January 2021

Smart Cities (or Cities 2.0) are an evolution in citizen habitation. In such cities, transport commuting is changing rapidly with the proliferation of contemporary vehicular technology. New models of vehicle ride sharing systems are changing the way citizens commute in their daily movement schedule. The use of a private vehicle per single passenger transportation is no longer viable in sustainable Smart Cities (SC) because of the vehicles’ resource allocation and urban pollution. The current research on car ride sharing systems is widely expanding in a range of contemporary technologies, however, without covering a multidisciplinary approach. In this paper, the focus is on performing a multidisciplinary research on car riding systems taking into consideration personalized user mobility behavior by providing next destination prediction as well as a recommender system based on riders’ personalized information. Specifically, it proposes a predictive vehicle ride sharing system for commuting, which has impact on the SC green ecosystem. The adopted system also provides a recommendation to citizens to select the persons they would like to commute with. An Artificial Intelligence (AI)-enabled weighted pattern matching model is used to assess user movement behavior in SC and provide the best predicted recommendation list of commuting users. Citizens are then able to engage a current trip to next destination with the more suitable user provided by the list. An experimented is conducted with real data from the municipality of New Philadelphia, in SC of Athens, Greece, to implement the proposed system and observe certain user movement behavior. The results are promising for the incorporation of the adopted system to other SCs.