bluuebunny/crossref_metadata_2025 huggingface dataset:
One-pot universal NicE-seq: all enzymatic downstream processing of 4% formaldehyde crosslinked cells for chromatin accessibility genomics
Authors: Vishnu Udayakumar S. , Estève Pierre-Olivier , Chin Hang Gyeong , Pradhan Sriharsa
Published: December 2021

Background

Accessible chromatin landscape allows binding of transcription factors, and remodeling of promoter and enhancer elements during development. Chromatin accessibility along with integrated multiomics approaches have been used for determining molecular subtypes of cancer in patient samples.

Results

One-pot Universal NicE-seq (One-pot UniNicE-seq) is an improved accessible chromatin profiling method that negate DNA purification and incorporate sonication free enzymatic fragmentation before library preparation and is suited to a variety of mammalian cells. One-pot UniNicE-seq is versatile, capable of profiling 4% formaldehyde fixed chromatin in as low as 25 fixed cells. Accessible chromatin profile is more efficient on formaldehyde-fixed cells using one-pot UniNicE-seq compared to Tn5 transposon mediated methods, demonstrating its versatility.

Conclusion

One-pot UniNicE-seq allows the entire process of accessible chromatin labeling and enrichment in one pot at 4% formaldehyde cross-linking conditions. It doesn’t require enzyme titration, compared to other technologies, since accessible chromatin is labelled with 5mC incorporation and deter degradation by nicking enzyme, thus opening the possibility for automation.
A method for monitoring the coupling evolution of microservice-based architectures
Authors: Apolinário Daniel R.F. , de França Breno B.N.
Published: December 2021

The microservice architecture is claimed to satisfy ongoing software development demands, such as resilience, flexibility, and velocity. However, developing applications based on microservices also brings some drawbacks, such as the increased software operational complexity. Recent studies have also pointed out the lack of methods to prevent problems related to the maintainability of these solutions. Disregarding established design principles during the software evolution may lead to the so-called architectural erosion, which can end up in a condition of unfeasible maintenance. As microservices can be considered a new architecture style, there are few initiatives to monitoring the evolution of software microservice-based architectures. In this paper, we introduce the SYMBIOTE method for monitoring the coupling evolution of microservice-based systems. More specifically, this method collects coupling metrics during runtime (staging or production environments) and monitors them throughout software evolution. The longitudinal analysis of the collected measures allows detecting an upward trend in coupling metrics that could represent signs of architectural degradation. To develop the proposed method, we performed an experimental analysis of the coupling metrics behavior using artificially generated data. The results of these experiment revealed the metrics behavior in different scenarios, providing insights to develop the analysis method for the identification of architectural degradation. We evaluated the SYMBIOTE method in a real-case open source project called Spinnaker. The results obtained in this evaluation show the relationship between architectural changes and upward trends in coupling metrics for most of the analyzed release intervals. Therefore, the first version of SYMBIOTE has shown potential to detect signs of architectural degradation during the evolution of microservice-based architectures.
The value of MRI compared to conventional radiography in analysing morphologic changes in the spine in axial spondyloarthritis
Authors: Adelsmayr Gabriel , Haidmayer Andreas , Spreizer Christopher , Janisch Michael , Quehenberger Franz , Klocker Eva , Graninger Winfried , Fuchsjäger Michael , Hermann Josef
Published: December 2021

Background

Imaging of morphologic changes in the vertebral spine in axial spondyloarthritis (SpA) is routinely performed with conventional radiography limited by superposition in the thoracic segments and radiation exposure. The objective was to assess the reliability of MRI compared to conventional radiography in depicting morphologic vertebral lesions in patients with axial SpA. Forty patients diagnosed with axial SpA were included in this cross-sectional study. Patients underwent MRI of the whole spine with T1-weighted and TIRM sequences in the sagittal plane and conventional radiography of the cervical and lumbar spine in lateral projections. Morphologic changes (syndesmophytes and erosions) in the anterior vertebral endplates on MRI and conventional radiography were independently evaluated by two radiologists. Inter-modality and interobserver agreement were calculated using Cohen’s Kappa.

Results

Inter-modality agreement was low for cervical and lumbar syndesmophytes and erosions (κ ≤ 0.2 ± 0.07–0.1). Interobserver agreement on conventional radiography was highest for cervical and lumbar anterior syndesmophytes/bridging (κ = 0.92 ± 0.02–0.03). Syndesmophytes in thoracic anterior vertebral units were the most frequent MRI finding with a high interobserver agreement (κ = 0.83 ± 0.05).

Conclusions

In imaging morphologic changes in the spine in patients with axial SpA, MRI was shown to be not an equivalent substitute but a complementary imaging modality to conventional radiography. Conventional radiography seems superior to depict morphologic cervical and lumbar vertebral changes compared to MRI, whereas MRI may visualise morphologic lesions in the thoracic spine.
CMR Characteristics, gene variants and long-term outcome in patients with left ventricular non-compaction cardiomyopathy
Authors: Zhou Di , Li Shijie , Sirajuddin Arlene , Wu Weichun , Huang Jinghan , Sun Xiaoxin , Zhao Shihua , Pu Jielin , Lu Minjie
Published: December 2021

Background

As the paucity of data focusing on evaluating cardiac structure and function in patients with or without gene mutation, this study was sought to investigate the correlation between genotype and cardiac magnetic resonance (CMR) phenotype in patients with left ventricular non-compaction cardiomyopathy (LVNC) and to explore prognostic relevance in this cohort if possible.

Methods

Patients with LVNC who underwent CMR and targeted gene sequencing between 2006 and 2016 were retrospectively evaluated. Demographic data, clinical presentation, genetic analysis, CMR data and follow-up data of all participants were collected.

Results

Compared to negative genotype (G−) group, patients with positive genotype (G+) had larger left atrial volume (LAV), and carriers of multiple variants had lower left ventricular (LV) ejection fraction and cardiac index, increased LV fibrosis, larger LA volume, reduced LV global circumferential strain, LA reservoir strain and booster pump strain (allp < 0.05). LA volume was able to discriminate patients with G + (allp < 0.05), as well as those with multiple genetic mutation (allp < 0.01). During a median follow-up of 5.1 years, Kaplan–Meier survival analysis revealed worse primary endpoint-free survival among carriers of multiple variants compared to G− group.

Conclusions

CMR feature tracking is a remarkable tool to evaluate implication, genetics cascade screen and predict outcome in LVNC population. LA volume is a sensitive and robust indicator for genetic mutational condition, of which facilities to guide clinical management and intensity of follow-up for patients and their relatives.
Intensity of arterial structure acquired by Silent MRA estimates cerebral blood flow
Authors: Hwang Zhen-An , Li Chia-Wei , Lin Chien-Yuan Eddy , Chen Jyh-Horng , Chen Chia-Yuen , Chan Wing P.
Published: December 2021

Background

Cerebral blood flow (CBF) and the morphology of the cerebral arteries are important for characterizing cerebrovascular disease. Silent magnetic resonance angiography (Silent MRA) is a MRA technique focusing on arterial structural delineation. This study was conducted to investigate the correlation between Silent MRA and CBF quantification, which has not yet been reported.

Methods

Both the Silent MRA and time-of-flight magnetic resonance angiography scans were applied in seventeen healthy participants to acquire the arterial structure and to find arterial intensities. Phase-contrast MRA (PC-MRA) was then used to perform the quantitative CBF measurement of 13 cerebral arteries. Due to different dataset baseline signal level of Silent MRA, the signal intensities of the selected 13 cerebral arteries were normalized to the selected ROIs of bilateral internal carotid arteries. The normalized signal intensities were used to determine the relationship between Silent MRA and CBF.

Results

The image intensity distribution of arterial regions generated by Silent MRA showed similar laminar shape as the phase distribution by PC-MRA (correlation coefficient > 0.62). Moreover, in both the results of individual and group-leveled analysis, the intensity value of arterial regions by Silent MRA showed positively correlation with the CBF by PC-MRA. The coefficient of determination (R2) of individual trends ranged from 0.242 to 0.956, and the R2 of group-leveled result was 0.550.

Conclusions

This study demonstrates that Silent MRA provides valuable CBF information despite arterial structure, rendering it a potential tool for screening for cerebrovascular disease.
Bone marrow derived-mesenchymal stem cell improves diabetes-associated fatty liver via mitochondria transformation in mice
Authors: Bi Youkun , Guo Xuejun , Zhang Mengqi , Zhu Keqi , Shi Chentao , Fan Baoqi , Wu Yanyun , Yang Zhiguang , Ji Guangju
Published: December 2021

Background

Non-alcoholic fatty liver disease (NAFLD) has become a global epidemic disease. Its incidence is associated with type 2 diabetes mellitus (T2DM). Presently, there is no approved pharmacological agents specially developed for NAFLD. One promising disease-modifying strategy is the transplantation of stem cells to promote metabolic regulation and repair of injury.

Method

In this study, a T2DM model was established through 28-week high-fat diet (HFD) feeding resulting in T2DM-associated NAFLD, followed by the injection of bone marrow mesenchymal stem cells (BMSCs). The morphology, function, and transfer of hepatocyte mitochondria were evaluated in both vivo and in vitro.

Results

BMSC implantation resulted in the considerable recovery of increasing weight, HFD-induced steatosis, liver function, and disordered glucose and lipid metabolism. The treatment with BMSC transplantation was accompanied by reduced fat accumulation. Moreover, mitochondrial transfer was observed in both vivo and vitro studies. And the mitochondria-recipient steatotic cells exhibited significantly enhanced OXPHOS activity, ATP production, and mitochondrial membrane potential, and reduced reactive oxygen species levels, which were not achieved by the blocking of mitochondrial transfer.

Conclusion

Mitochondrial transfer from BMSCs is a feasible process to combat NAFLD via rescuing dysfunction mitochondria, and has a promising therapeutic effect on metabolism-related diseases.
ChemTables: a dataset for semantic classification on tables in chemical patents
Authors: Zhai Zenan , Druckenbrodt Christian , Thorne Camilo , Akhondi Saber A. , Nguyen Dat Quoc , Cohn Trevor , Verspoor Karin
Published: December 2021

Chemical patents are a commonly used channel for disclosing novel compounds and reactions, and hence represent important resources for chemical and pharmaceutical research. Key chemical data in patents is often presented in tables. Both the number and the size of tables can be very large in patent documents. In addition, various types of information can be presented in tables in patents, including spectroscopic and physical data, or pharmacological use and effects of chemicals. Since images of Markush structures and merged cells are commonly used in these tables, their structure also shows substantial variation. This heterogeneity in content and structure of tables in chemical patents makes relevant information difficult to find. We therefore propose a new text mining task of automatically categorising tables in chemical patents based on their contents. Categorisation of tables based on the nature of their content can help to identify tables containing key information, improving the accessibility of information in patents that is highly relevant for new inventions. For developing and evaluating methods for the table classification task, we developed a new dataset, calledChemTables, which consists of 788 chemical patent tables with labels of their content type. We introduce this data set in detail. We further establish strong baselines for the table classification task in chemical patents by applying state-of-the-art neural network models developed for natural language processing, including TabNet, ResNet and Table-BERT onChemTables. The best performing model, Table-BERT, achieves a performance of 88.66 micro-averaged$$F_1$$F1score on the table classification task. TheChemTablesdataset is publicly available athttps://doi.org/10.17632/g7tjh7tbrj.3, subject to the CC BY NC 3.0 license. Code/models evaluated in this work are in a Github repositoryhttps://github.com/zenanz/ChemTables.
Processing binding data using an open-source workflow
Authors: Samuel Errol L. G. , Holmes Secondra L. , Young Damian W.
Published: December 2021

The thermal shift assay (TSA)—also known as differential scanning fluorimetry (DSF), thermofluor, and Tm shift—is one of the most popular biophysical screening techniques used in fragment-based ligand discovery (FBLD) to detect protein–ligand interactions. By comparing the thermal stability of a target protein in the presence and absence of a ligand, potential binders can be identified. The technique is easy to set up, has low protein consumption, and can be run on most real-time polymerase chain reaction (PCR) instruments. While data analysis is straightforward in principle, it becomes cumbersome and time-consuming when the screens involve multiple 96- or 384-well plates. There are several approaches that aim to streamline this process, but most involve proprietary software, programming knowledge, or are designed for specific instrument output files. We therefore developed an analysis workflow implemented in the Konstanz Information Miner (KNIME), a free and open-source data analytics platform, which greatly streamlined our data processing timeline for 384-well plates. The implementation is code-free and freely available to the community for improvement and customization to accommodate a wide range of instrument input files and workflows.

Graphical Abstract
Low-cost production and application of lipopeptide for bioremediation and plant growth by Bacillus subtilis SNW3
Authors: Umar Aiman , Zafar Aneeqa , Wali Hasina , Siddique Meh Para , Qazi Muneer Ahmed , Naeem Afshan Hina , Malik Zulfiqar Ali , Ahmed Safia
Published: December 2021

At present time, every nation is absolutely concern about increasing agricultural production and bioremediation of petroleum-contaminated soil. Hence, with this intention in the current study potent natural surfactants characterized as lipopeptides were evaluated for low-cost production by Bacillus subtilis SNW3, previously isolated from the Fimkessar oil field, Chakwal Pakistan. The significant results were obtained by using substrates in combination (white beans powder (6% w/v) + waste frying oil (1.5% w/v) and (0.1% w/v) urea) with lipopeptides yield of about 1.17 g/L contributing 99% reduction in cost required for medium preparation. To the best of our knowledge, no single report is presently describing lipopeptide production by Bacillus subtilis using white beans powder as a culture medium. Additionally, produced lipopeptides display great physicochemical properties of surface tension reduction value (SFT = 28.8 mN/m), significant oil displacement activity (ODA = 4.9 cm), excessive emulsification ability (E24 = 69.8%), and attains critical micelle concentration (CMC) value at 0.58 mg/mL. Furthermore, biosurfactants produced exhibit excellent stability over an extensive range of pH (1–11), salinity (1–8%), temperature (20–121°C), and even after autoclaving. Subsequently, produced lipopeptides are proved suitable for bioremediation of crude oil (86%) and as potent plant growth-promoting agent that significantly (P < 0.05) increase seed germination and plant growth promotion of chili pepper, lettuce, tomato, and pea maximum at a concentration of (0.7 g/100 mL), showed as a potential agent for agriculture and bioremediation processes by lowering economic and environmental stress.

Graphical Abstract
Single-cell RNA-sequencing reveals distinct immune cell subsets and signaling pathways in IgA nephropathy
Authors: Zeng Honghui , Wang Le , Li Jiajia , Luo Siweier , Han Qianqian , Su Fang , Wei Jing , Wei Xiaona , Wu Jianping , Li Bin , Huang Jingang , Tang Patrick , Cao Chunwei , Zhou Yiming , Yang Qiongqiong
Published: December 2021

Background

IgA nephropathy (IgAN) is the most common primary glomerulonephritis globally. Increasing evidence suggests the importance of host immunity in the development of IgAN, but its dynamics during the early stage of IgAN are still largely unclear.

Results

Here we successfully resolved the early transcriptomic changes in immune cells of IgAN by conducting single-cell RNA-sequencing (scRNA-seq) with peripheral blood mononuclear cells. The differentially expressed genes (DEGs) between control and IgAN were predominantly enriched in NK cell-mediated cytotoxicity and cell killing pathways. Interestingly, we discovered that the number and cytotoxicity of NK cells are significantly reduced in IgAN patients, where both the number and marker genes of NK cells were negatively associated with the clinical parameters, including the levels of urine protein creatinine ratio (UPCR), serum galactose-deficient IgA1 and IgA. A distinctive B cell subset, which had suppressed NFκB signaling was predominantly in IgAN and positively associated with disease progression. Moreover, the DEGs of B cells were enriched in different viral infection pathways. Classical monocytes also significantly changed in IgAN and a monocyte subset expressing interferon-induced genes was positively associated with the clinical severity of IgAN. Finally, we identified vast dynamics in intercellular communications in IgAN.

Conclusions

We dissected the immune landscape of IgAN at the single-cell resolution, which provides new insights in developing novel biomarkers and immunotherapy against glomerulonephritis.
NOMA resource allocation method in IoV based on prioritized DQN-DDPG network
Authors: He Mengli , Li Yue , Wang Xiaofei , Liu Zelong
Published: December 2021

To meet the demands of massive connections in the Internet-of-vehicle communications, non-orthogonal multiple access (NOMA) is utilized in the local wireless networks. In NOMA technique, various optimization methods have been proposed to provide optimal resource allocation, but they are limited by computational complexity. Recently, the deep reinforcement learning network is utilized for resource optimization in NOMA system, where a uniform sampled experience replay algorithm is used to reduce the correlation between samples. However, the uniform sampling ignores the importance of sample. To this point, this paper proposes a joint prioritized DQN user grouping and DDPG power allocation algorithm to maximize the system sum rate. At the user grouping stage, a prioritized sampling method based on TD-error (temporal-difference error) is proposed. At the power allocation stage, to deal with the problem that DQN cannot process continuous tasks and needs to quantify power into discrete form, a DDPG network is utilized. Simulation results show that the proposed algorithm with prioritized sampling can increase the learning rate and perform a more stable training process. Compared with the previous DQN algorithm, the proposed method improves the sum rate of the system by 2% and reaches 94% and 93% of the exhaustive search algorithm and optimal iterative power optimization algorithm, respectively. Although the sum rate is improved by only 2%, the computational complexity is reduced by 43% and 64% compared to the exhaustive search algorithm and the optimal iterative power optimization algorithm, respectively.
Bergman spaces with exponential type weights
Authors: Arroussi Hicham
Published: December 2021

For $1\le p<\infty $
1
≤
p
<
∞
, let $A^{p}_{\omega }$

A
ω
p

be the weighted Bergman space associated with an exponential type weight ω satisfying
$$ \int _{{\mathbb{D}}} \bigl\vert K_{z}(\xi ) \bigr\vert \omega (\xi )^{1/2} \,dA(\xi ) \le C \omega (z)^{-1/2}, \quad z\in {\mathbb{D}}, $$

∫
D

|

K
z

(
ξ
)
|
ω

(
ξ
)

1
/
2

d
A
(
ξ
)
≤
C
ω

(
z
)

−
1
/
2

,

z
∈
D
,
where $K_{z}$

K
z

is the reproducing kernel of $A^{2}_{\omega }$

A
ω
2

. This condition allows us to obtain some interesting reproducing kernel estimates and more estimates on the solutions of the ∂̅-equation (Theorem 2.5) for more general weight $\omega _{*}$

ω
∗

. As an application, we prove the boundedness of the Bergman projection on $L^{p}_{\omega }$

L
ω
p

, identify the dual space of $A^{p}_{\omega }$

A
ω
p

, and establish an atomic decomposition for it. Further, we give necessary and sufficient conditions for the boundedness and compactness of some operators acting from $A^{p}_{\omega }$

A
ω
p

into $A^{q}_{\omega }$

A
ω
q

, $1\le p,q<\infty $
1
≤
p
,
q
<
∞
, such as Toeplitz and (big) Hankel operators.
Existence of groundstates for Choquard type equations with Hardy–Littlewood–Sobolev critical exponent
Authors: Li Xiaowei , Wang Feizhi
Published: December 2021

In this paper, we consider a class of Choquard equations with Hardy–Littlewood–Sobolev lower or upper critical exponent in the whole space $\mathbb{R}^{N}$

R
N

. We combine an argument of L. Jeanjean and H. Tanaka (see (Proc. Am. Math. Soc. 131:2399–2408, 2003) with a concentration–compactness argument, and then we obtain the existence of ground state solutions, which extends and complements the earlier results.
What evidence exists on the effects of competition on trees’ responses to climate change? A systematic map protocol
Authors: Magalhães Juliana G. de S. , Amoroso Mariano M. , Larson Bruce C.
Published: December 2021

Background

Projections of climate change impacts upon forests are likely inaccurate if based on the premise that only climate controls tree growth. Species interactions control growth, but most research has ignored these effects on how trees respond to climate change. Climate change is inducing natural species selection. However, this selection does not occur at the community level. Species selection starts with competition amongst individual trees. Competition is an individual-to-individual antagonistic interaction that, if severe, can constrain the presence of trees within a particular environment. Thus, climate change impacts individual tree selection within forests. Projecting climate change impacts on forests should account for the effects of climate on tree growth and the effects of competition. The inclusion of competition can increase the predictive power of simulations.

Methods

We propose a protocol to systematically map the available literature on climate change impacts on forests and produce a comprehensive list of methods applied to measure competition and model the competition effects on tree growth responses to climate change. This systematic map is not limited to any country or continent or specific tree species or forest type. The scope of the search focuses on time (when the evidence was published), location (geographic location of the evidence) and research design (competition indices and modelling methods). We will evaluate articles at three levels: title, abstract and full text. We will conduct a full-text assessment on all articles that pass a screening at the title and abstract stages. We will report the extracted evidence in a narrative synthesis to summarize the evidence’s trends and report knowledge gaps.
Estimating ecotoxicological effects of chemicals on tropical reef-building corals; a systematic review protocol
Authors: Ouédraogo Dakis-Yaoba , Perceval Olivier , Ferrier-Pagès Christine , Domart-Coulon Isabelle , Hédouin Laetitia , Burga Karen , Guillaume Mireille M. M. , Calvayrac Christophe , Castelin Magalie , Reyjol Yorick , Sordello Romain
Published: December 2021

Background

Tropical coral reefs cover only ca. 0.1% of the Earth’s surface but host an outstanding biodiversity and provide important ecosystem services to millions of people living nearby. They are currently threatened by global (e.g., climate change) and local (e.g., chemical pollution) stressors that interact in different ways. While global stressors cannot be mitigated by local actions alone, local stressors can be reduced through ecosystem management. A systematic map on the impacts of chemicals arising from anthropogenic activities on tropical reef-building corals, which are the main engineer species of reef ecosystems, was published in 2021. This systematic map gathered an abundant literature (908 articles corresponding to 7937 studies), and identified four well-represented subtopics, amenable to relevant full syntheses. Here, we focused on one of the four subtopics: we aimed to systematically review the evidence on the ecotoxicological effects of chemicals on tropical reef-building corals.

Methods

The evidence will be identified from the recent systematic map on the impacts of chemicals arising from anthropogenic activities on tropical reef-building corals. Especially, all studies in the map database corresponding to the knowledge cluster “evidence on the ecotoxicological effects of chemicals on corals” will be selected. To identify the evidence produced since then, a search update will be performed using a subset of the search string used for the systematic map, and titles, abstracts and full-texts will be screened according to the criteria defining the selected cluster of the map. In addition, as the eligibility criteria for the systematic review are narrower than those used to define the cluster in the systematic map, additional screening will be carried out. The included studies will then be critically appraised and a low, medium, or high risk of bias will be assigned to each study. Data will be extracted from studies and synthesised according to a strategy depending on the type of exposure and outcome. Synthesis will be mainly quantitative but also narrative, aiming to identify toxicity thresholds of chemicals for corals.
A fresh look at the role of spiramycin in preventing a neglected disease: meta-analyses of observational studies
Authors: Montoya Jose G. , Laessig Katherine , Fazeli Mir Sohail , Siliman Gaye , Yoon Sophie S. , Drake-Shanahan Elizabeth , Zhu Chengyue , Akbary Akbar , McLeod Rima
Published: December 2021

Purpose

We aimed to investigate the effect of antepartum treatment with spiramycin with or without subsequent pyrimethamine–sulfonamide–folinic acid, compared to no treatment, on the rate of mother-to-child transmission (MTCT) of Toxoplasma gondii (T. gondii) and incidence/severity of sequelae in the offspring.

Methods

Embase and PubMed were searched for literature on spiramycin in pregnant women suspected/diagnosed with T. gondii infection. Meta-analyses were performed using random-effects model.

Results

Thirty-three studies (32 cohorts and 1 cross-sectional study), with a total of 15,406 mothers and 15,250 offspring, were pooled for analyses. The MTCT rate for all treated patients was significantly lower than the untreated [19.5% (95% CI 14–25.5%) versus 50.7% (95% CI 31.2–70%), p < 0.001]. The transmission rate in patients on spiramycin monotherapy was also significantly lower than untreated [17.6% (95% CI 9.9–26.8%) versus 50.7% (95% CI 31.2–70%), p < 0.001].

Conclusion

Results indicate significant reduction in MTCT rates following spiramycin treatment of suspected/diagnosed maternal T. gondii infection.
Update on pathogenesis and diagnosis flow of normoalbuminuric diabetes with renal insufficiency
Authors: Deng Le , Li Wenjie , Xu Gaosi
Published: December 2021

In recent decades, the prevalence of diabetic kidney disease has remained stable and appears to be a wide heterogeneity. Normoalbuminuric diabetes with renal insufficiency, which is characterized by a decline in the glomerular filtration rate in the absence of albuminuria, has been identified as an albuminuria-independent phenotype of diabetic kidney disease. Epidemiological data demonstrate that normoalbuminuric phenotype is prevalent. Compared to albuminuric phenotype, normoalbuminuric phenotype has distinct clinical characteristics and a wide heterogeneity of pathological features. Currently, the pathogenesis of normoalbuminuric phenotype remains unclear. Additionally, the flow of diagnosing normoalbuminuric phenotype is not perfect. In this article, we review the latest studies addressing the epidemiology, clinical characteristics, and pathology of normoalbuminuric phenotype. Based on the studies of clinical features and renal histopathologic changes, we attempt to propose an underlying pathogenesis model and a flow chart for diagnosing normoalbuminuric phenotype.
Findings from a web content analysis of resources targeting sporting coaches aimed at educating or upskilling on eating disorders and disordered eating in athletes
Authors: Haslam Rebecca L. , Clarke Erin D. , Gray Scarlett , Gearon Rachel , Pursey Kirrilly
Published: December 2021

Background

Eating disorders (ED) and disordered eating (DE) are highly prevalent in athletes. Coaches can play a role in the prevention of EDs and DE behaviours and are well placed to support athletes with an ED. However, coaches feel under-qualified and lack time and resources for supporting athletes and it is unclear the quality of training and resources available to upskill coaches in this space. Therefore, a web-based content analysis was undertaken to determine the type and source of online education resources currently available to coaches to help identify, prevent, manage and refer on for ED/DE behaviours.

Methods

Three major search engines were searched using a combination of the following terms: (1) DE or ED resource and (2) coaches or sport. Included websites were specific for DE/EDs in athletes; targeted at coaches or sporting organisations; written in the English language; and published by a reputable site.

Results

Twenty four out of 600 websites met inclusion criteria. The main reasons for exclusion were irreputable sites and websites not targeting coaches. The majority of included webpages were from professional bodies (n = 17) and targeted coaches (n = 24) and sporting organisations (n = 15), with an average quality rating of 4.2 out of 6. All websites provided educational resources but none provided official training. The most common topics discussed on these websites was ED/DE signs and symptoms (n = 17), and the effects of ED/DE on performance, mental and physical health (n = 11).

Conclusion

Few reputable online resources were identified in the current review. There is a need for more comprehensive education and training resources aimed at coaches and athletic organisations to help prevent, identify, manage and refer on for ED/DE behaviours.
Patho-mechanisms of the origins of bronchopulmonary dysplasia
Authors: Sahni Mitali , Bhandari Vineet
Published: December 2021

Bronchopulmonary dysplasia (BPD) continues to be one of the most common complications of prematurity, despite significant advancement in neonatology over the last couple of decades. The new BPD is characterized histopathologically by impaired lung alveolarization and dysregulated vascularization. With the increased survival of extremely preterm infants, the risk for the development of BPD remains high, emphasizing the continued need to understand the patho-mechanisms that play a role in the development of this disease. This brief review summarizes recent advances in our understanding of the maldevelopment of the premature lung, highlighting recent research in pathways of oxidative stress-related lung injury, the role of placental insufficiency, growth factor signaling, the extracellular matrix, and microRNAs.
Dispersal distance is driven by habitat availability and reproductive success in Northern Great Plains piping plovers
Authors: Swift Rose J. , Anteau Michael J. , Ellis Kristen S. , Ring Megan M. , Sherfy Mark H. , Toy Dustin L.
Published: December 2021

Background

Dispersal is a critical life history strategy that has important conservation implications, particularly for at-risk species with active recovery efforts and migratory species. Both natal and breeding dispersal are driven by numerous selection pressures, including conspecific competition, individual characteristics, reproductive success, and spatiotemporal variation in habitat. Most studies focus on dispersal probabilities, but the distance traveled can affect survival, fitness, and even metapopulation dynamics.

Methods

We examined sources of variation in dispersal distances with 275 natal dispersal and 1335 interannual breeding events for piping plovers (Charadrius melodus) breeding in the Northern Great Plains between 2014 and 2019.

Results

Natal dispersal was on average longer (mean: 81.0 km, median: 53 km) than adult breeding movements (mean: 23.7 km, median: 1 km). Individuals moved the shortest distances when hatched, previously nested, or settling on river habitats. When more habitat was available on their natal area than in the year prior, hatch-year birds moved shorter distances to their first breeding location. Similarly, adults also moved shorter distances when more habitat was available at the settling site and when in closer proximity to other known nesting areas. Additionally, adult movement distance was shorter when successfully hatching a nest the year prior, retaining a mate, or initiating a current nest earlier.

Conclusion

Habitat availability appears to be associated with dispersal distance for both hatch-year and adult piping plovers. Conservation efforts that integrate dispersal distances may benefit from maintaining nesting habitat within close proximity to other areas for adults and a network of clustered sites spread out across a larger landscape for natal dispersal.
Migration distance affects how closely Eurasian wigeons follow spring phenology during migration
Authors: van Toor Mariëlle L. , Kharitonov Sergey , Švažas Saulius , Dagys Mindaugas , Kleyheeg Erik , Müskens Gerard , Ottosson Ulf , Žydelis Ramunas , Waldenström Jonas
Published: December 2021

Background

The timing of migration for herbivorous migratory birds is thought to coincide with spring phenology as emerging vegetation supplies them with the resources to fuel migration, and, in species with a capital breeding strategy also provides individuals with energy for use on the breeding grounds. Individuals with very long migration distances might however have to trade off between utilising optimal conditions en route and reaching the breeding grounds early, potentially leading to them overtaking spring on the way. Here, we investigate whether migration distance affects how closely individually tracked Eurasian wigeons follow spring phenology during spring migration.

Methods

We captured wigeons in the Netherlands and Lithuania and tracked them throughout spring migration to identify staging sites and timing of arrival. Using temperature-derived indicators of spring phenology, we investigated how maximum longitude reached and migration distance affected how closely wigeons followed spring. We further estimated the impact of tagging on wigeon migration by comparing spring migratory timing between tracked individuals and ring recovery data sets.

Results

Wigeons migrated to locations between 300 and 4000 km from the capture site, and migrated up to 1000 km in a single day. We found that wigeons migrating to more north-easterly locations followed spring phenology more closely, and increasingly so the greater distance they had covered during migration. Yet we also found that despite tags equalling only around 2% of individual’s body mass, individuals were on average 11–12 days slower than ring-marked individuals from the same general population.

Discussion

Overall, our results suggest that migratory strategy can vary dependent on migration distance within species, and even within the same migratory corridor. Individual decisions thus depend not only on environmental cues, but potentially also trade-offs made during later life-history stages.
Clinically aggressive pediatric spinal ependymoma with novel MYC amplification demonstrates molecular and histopathologic similarity to newly described MYCN-amplified spinal ependymomas
Authors: Shatara Margaret , Schieffer Kathleen M. , Klawinski Darren , Thomas Diana L. , Pierson Christopher R. , Sribnick Eric A. , Jones Jeremy , Rodriguez Diana P. , Deeg Carol , Hamelberg Elizabeth , LaHaye Stephanie , Miller Katherine E. , Fitch James , Kelly Benjamin , Leraas Kristen , Pfau Ruthann , White Peter , Magrini Vincent , Wilson Richard K. , Mardis Elaine R. , Abdelbaki Mohamed S. , Finlay Jonathan L. , Boué Daniel R. , Cottrell Catherine E. , Ghasemi David R. , Pajtler Kristian W. , Osorio Diana S.
Published: December 2021

Primary spinal cord tumors contribute to ≤ 10% of central nervous system tumors in individuals of pediatric or adolescent age. Among intramedullary tumors, spinal ependymomas make up ~ 30% of this rare tumor population. A twelve-year-old male presented with an intradural, extramedullary mass occupying the dorsal spinal canal from C6 through T2. Gross total resection and histopathology revealed a World Health Organization (WHO) grade 2 ependymoma. He recurred eleven months later with extension from C2 through T1-T2. Subtotal resection was achieved followed by focal proton beam irradiation and chemotherapy. Histopathology was consistent with WHO grade 3 ependymoma. Molecular profiling of the primary and recurrent tumors revealed a novel amplification of the MYC (8q24) gene, which was confirmed by fluorescence in situ hybridization studies. Although MYC amplification in spinal ependymoma is exceedingly rare, a newly described classification of spinal ependymoma harboring MYCN (2p24) amplification (SP-MYCN) has been defined by DNA methylation-array based profiling. These individuals typically present with a malignant progression and dismal outcomes, contrary to the universally excellent survival outcomes seen in other spinal ependymomas. DNA methylation array-based classification confidently classified this tumor as SP-MYCN ependymoma. Notably, among the cohort of 52 tumors comprising the SP-MYCN methylation class, none harbor MYC amplification, highlighting the rarity of this genomic amplification in spinal ependymoma. A literature review comparing our individual to reported SP-MYCN tumors (n = 26) revealed similarities in clinical, histopathologic, and molecular features. Thus, we provide evidence from a single case to support the inclusion of MYC amplified spinal ependymoma within the molecular subgroup of SP-MYCN.
Environmental and anthropogenic gravity contributions at the Þeistareykir geothermal field, North Iceland
Authors: Forster Florian , Güntner Andreas , Jousset Philippe , Reich Marvin , Männel Benjamin , Hinderer Jacques , Erbas Kemal
Published: December 2021

Continuous high-resolution gravimetry is increasingly used to monitor mass distribution changes in volcanic, hydrothermal or other complex geosystems. To quantify the often small target signals, gravity contributions from, e.g. atmospheric mass changes, global and local hydrology should be accounted for. We set up three iGrav superconducting gravity meters for continuous monitoring of the Þeistareykir geothermal field in North Island. Additionally, we installed a set of hydrometeorological sensors at each station for continuous observation of local pressure changes, soil moisture, snow and vertical surface displacement. We show that the contribution of these environmental parameters to the gravity signal does not exceed 10 µGal (1 µGal = 10–8 m s−2), mainly resulting from vertical displacement and snow accumulation. The seasonal gravity contributions (global atmosphere, local and global hydrology) are in the order of ± 2 µGal at each station. Using the environmental observations together with standard gravity corrections for instrumental drift and tidal effects, we comprehensively reduced the iGrav time-series. The gravity residuals were compared to groundwater level changes and geothermal mass flow rates (extraction and injection) of the Þeistareykir power plant. The direct response of the groundwater levels and a time-delayed response of the gravity signal to changes in extraction and injection suggest that the geothermal system is subject to a partially confined aquifer. Our observations indicate that a sustainable “equilibrium” state of the reservoir is reached at extraction flow rates below 240 kg s−1and injection flow rates below 160 kg s−1. For a first-order approximation of the gravity contributions from extracted and injected masses, we applied a simplified forward gravity model. Comparison to the observed gravity signals suggest that most of the reinjected fluid is drained off through the nearby fracture system.
Measuring the impact of risk on LCOE (levelized cost of energy) in geothermal technology
Authors: Park Soojin , Langat Antony , Lee Kyuhwan , Yoon Yongbeum
Published: December 2021

Geothermal technology has a high level of uncertainty and, thus, requires thorough risk analysis for economic decisions. The levelized cost of energy (LCOE) is a basic economic analysis widely used in determining an investment or energy mix. Many reputable institutions and government agencies provide LCOE, to which they apply different levels of discount rates to reflect project risk. To this end, the weighted average cost of capital (WACC) is frequently used as a proxy for project risk-adjusted discount rate. However, whether using a higher discount rate for a riskier project is appropriate in calculating LCOE has not been scrutinized. The purpose of this paper is to propose a certainty equivalent method of LCOE as an alternative way for considering risk. We present a theoretical background and formula based on the utility theory, improving the probabilistic LCOE estimation methodology of previous studies. We also perform scenario analysis to show how the certainty equivalent model changes LCOE reflecting different level of risk of the individual variables, which traditional LCOE does not. Additionally, we suggest that the traditional LCOE should be used prudently, recognizing it can distort the result when an individual project has a different level of risk from the industry average.
Biochemical response and nutrient uptake of two arbuscular mycorrhiza-inoculated chamomile varieties under different osmotic stresses
Authors: Ebrahimi Fatemeh , Salehi Amin , Movahedi Dehnavi Mohsen , Mirshekari Amin , Hamidian Mohammad , Hazrati Saeid
Published: December 2021

Background

Water-deficit stress is known as one of the most severe environmental stresses affecting the growth of plants through marked reduction of water uptake, which leads to osmotic stress by lowering water potential. Adopting appropriate varieties using soil microorganisms, such as arbuscular mycorrhiza (AM) fungi, can significantly reduce the adverse effects of water deficiency. This study aimed to evaluate the role of Funneliformis mosseae on nutrient uptake and certain physiological traits of two chamomile varieties, namely Bodgold (Bod) and Soroksári (Sor) under osmotic stress. For pot culture, a factorial experiment was performed in a completely randomized design with three factors: osmotic stress (PEG 6000) was applied along with Hoagland solution at three levels (0, -0.4 and -0.8 MPa), two German chamomile varieties (Bodgold (Bod) and Soroksari (Sor)), and AM inoculation (Funneliformis mosseae species (fungal and non-fungal)) at four replications in perlite substrate.

Results

Osmotic stress significantly reduced the uptake of macro-nutrients (N and P) and micro-nutrients (Fe, Cu, Mn, and Zn) in the shoots and roots. Moreover, the level of osmolytes (total soluble sugars and proline) and the activity of antioxidant enzymes in the shoots of both varieties increased under osmotic stress. Regarding the Sor variety, the level of these compounds was more satisfactory. AM improved plant nutrition uptake and osmolyte contents while enhancing antioxidant enzymes and reducing the adverse effects of osmotic stress. Under osmotic stress, the growth and total dry weight were improved upon AM inoculation.

Conclusions

In general, inoculation of chamomile with AM balanced the uptake of nutrients and increased the level of osmolytes and antioxidant enzymes; hence, it improved plant characteristics under osmotic stress in both varieties. However, it was found to be more effective in reducing stress damages in the Sor variety.
Review: the effect of light on the key pigment compounds of photosensitive etiolated tea plant
Authors: Yue Cuinan , Wang Zhihui , Yang Puxiang
Published: December 2021

Background

Light is the ultimate energy source of plant photosynthesis, which has an important impact on the growth, development, physiology and biochemistry of tea plant. Photosensitive etiolated tea plant belongs to a kind of colored leaf plant, which is a physiological response to light intensity. Compared with conventional green bud and leaf of tea plant, the accumulation of pigment compounds (chlorophyll and carotenoids, etc.) closely related to a series of reactions of photosynthesis in photosensitive etiolated tea plant is reduced, resulting in the difference of leaf color of tea. This specific tea resource has high application value, among which high amino acid is one of its advantages. It can be used to process high-quality green tea with delicious taste and attractive aroma, which has been widely attention. The mechanism of the color presentation of the etiolated mutant tea leaves has been given a high topic and attention, especially, what changes have taken place in the pigment compounds of tea leaves caused by light, which makes the leaves so yellow. At present, there have been a lot of research and reports.

Purpose of the review

We describe the metabolism and differential accumulation of key pigment compounds affecting the leaf color of photosensitive etiolated tea that are triggered by light, and discuss the different metabolism and key regulatory sites of these pigments in different light environments in order to understand the “discoloration” matrix and mechanism of etiolated tea resources, answer the scientific question between leaf color and light. It provides an important strategy for artificial intervention of discoloration of colored tea plant.

Conclusion

The differential accumulation of pigment compounds in tea plant can be induced phytochrome in response to the change of light signal. The synthesis of chlorophyll in photoetiolated tea plants is hindered by strong light, among which, the sites regulated by coproporphyrinogen III oxidase and chlorophyllide a oxidase is sensitive to light and can be inhibited by strong light, resulting in the aggravation of leaf etiolation. The phenomenon can be disappeared or weakened by shading or reducing light intensity, and the leaf color is greenish, but the increase of chlorophyll-b accumulation is more than that of chlorophyll-a. The synthesis of carotenoids is inhibited strong light, and high the accumulation of carotenoids is reduced by shading. Most of the genes regulating carotenoids are up-regulated by moderate shading and down-regulated by excessive shading. Therefore, the accumulation of these two types of pigments in photosensitive etiolated tea plants is closely related to the light environment, and the leaf color phenotype shape of photosensitive etiolated tea plants can be changed by different light conditions, which provides an important strategy for the production and management of tea plant.