bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Figure 6 from NEDD9 Regulates Actin Dynamics through Cortactin Deacetylation in an AURKA/HDAC6–Dependent Manner
Authors: Kozyreva Varvara K. , McLaughlin Sarah L. , Livengood Ryan H. , Calkins Robin A. , Kelley Laura C. , Rajulapati Anuradha , Ice Ryan J. , Smolkin Matthew B. , Weed Scott A. , Pugacheva Elena N.
Published: April 2023

PDF - 608K, Supplementary Figure 6. A. Representative BLI of lungs dissected from 4 groups of mice after 2 weeks of treatment as in Fig.7A-B. B. Quantification of BLI of dissected lungs from mice as in (A); 3 independent experiments, n=6/group, Average radiance +/-S.E.M. One-way ANOVA with pairwise comparison *p=0.0005 for Vehicle/MLN; **p=0.0157 for Vehicle/TubA; ***p=0.002 for Vehicle/MLN+TubA; ns (p=0.1052) for TubA/TubA+MLN. C. Representative images of immunofluorescent staining of MDA-MB-231 cells treated with vehicle (control), Trichostatin A (TSA), Tubastatin A (TubA) and ACY-1215 (ACY) with anti-acetylated tubulin antibodies (yellow) and DNA (DAPI, blue). Final concentrations as indicated in the figure. Scale bar is 10μm. D. Top: Immunoprecipitation of acetylated CTTN with anti-acetylated lysine antibodies or non specific rabbit IgG (control) from lysates of cells treated as in C; control (no treatment or DMSO treated), Trichostatin A (TSA, 1mg/ml), Tubacin (20μM), Tubastatin A (TubA, 20μm), ACY1215 (ACY, 2μm). Wester blot with anti-CTTN antibodies. Bottom: WB of WCL used for IP with anti-CTTN and GAPDH antibodies. E. Representative BLI of lungs dissected from 4 groups of mice intravenously injected with the MDA-MB-231LN cells after 2 weeks of treatment. F. Quantification of BLI of dissected lungs from mice as in (E); 3 independent experiments, n=5/group, Average radiance +/-S.E.M. One-way ANOVA with pairwise comparison *p=0.0121 for Vehicle/MLN; **p=0.0203 for Vehicle/TubA; ***p=0.0121 for Vehicle/MLN+TubA; ns (p=0.8153) for TubA/TubA+MLN. G. Quantification of the number of metastases in the lungs of animals at 2 weeks post treatment as in F using serial sections of the lungs and H&E staining. Results are a mean number of metastases per mm2 +/-S.E.M, one-way ANOVA *p<0.0001 for Vehicle/MLN, **p=0.0017 Vehicle/TubA, ***p<0.0001 for Vehicle/MLN+TubA, ****p=0.0002 for TubA/MLN+TubA.
Supplementary Figure 1 from Prognostic Relevance of Tumor Purity and Interaction with MGMT Methylation in Glioblastoma
Authors: Schulze Heuling Eva , Knab Felix , Radke Josefine , Eskilsson Eskil , Martinez-Ledesma Emmanuel , Koch Arend , Czabanka Marcus , Dieterich Christoph , Verhaak Roel G. , Harms Christoph , Euskirchen Philipp
Published: April 2023

Tumor purity of matched tumor/cell line pairs: Tumor purity of GBM bulk tumor samples and matched gliomasphere cell lines was determined either using ESTIMATE for deconvolution of gene expression profiles (A) or pTERT MAF (B, C). Connected dots indicate matched pairs.
Supplementary Figures S1 and S2 from Biochemical and Structural Analysis of Common Cancer-Associated KRAS Mutations
Authors: Hunter John C. , Manandhar Anuj , Carrasco Martin A. , Gurbani Deepak , Gondi Sudershan , Westover Kenneth D.
Published: April 2023

Supplementary Figures S1 and S2. Figure S1. -GTP hydrolysis rate is stimulated by P120GAP. Figure S2. - Model of Intrinsic GTP hydrolysis.
Supplementary Figure 1 from NEDD9 Regulates Actin Dynamics through Cortactin Deacetylation in an AURKA/HDAC6–Dependent Manner
Authors: Kozyreva Varvara K. , McLaughlin Sarah L. , Livengood Ryan H. , Calkins Robin A. , Kelley Laura C. , Rajulapati Anuradha , Ice Ryan J. , Smolkin Matthew B. , Weed Scott A. , Pugacheva Elena N.
Published: April 2023

PDF - 86K, Supplementary Figure 1. Kymography measurements procedure. For each lamellipodia following measurements are acquired and calculated based on the following formulas: Protrusion max extension =X*P, !m; Persistence of protrusion =(Y1+Y2)*(sec/frames), sec/frames; Protrusion velocity =(X/Y1)*(frames/sec)*P, !m/sec; Where: X, Y1, Y2 as indicated on the image, P- is 1 pixel length =0.21!m, sec- is movie total time (=7200 sec), frames- is number of frames captured (=121 frames).
Supplementary Figures S3 and S4 from Key Survival Factor, Mcl-1, Correlates with Sensitivity to Combined Bcl-2/Bcl-xL Blockade
Authors: Williams Michelle M. , Lee Linus , Hicks Donna J. , Joly Meghan M. , Elion David , Rahman Bushra , McKernan Courtney , Sanchez Violeta , Balko Justin M. , Stricker Thomas , Estrada Monica Valeria , Cook Rebecca S.
Published: April 2023

S3. Representative images of cells grown in 3D Matrigel for 14D and treated with ABT-263 (1.0 uM). S4. Whole cell lysates from cells treated with ABT-263 (1.0uM) for 0=24 hours.
Supplementary Figure S5 from Sulfiredoxin Promotes Colorectal Cancer Cell Invasion and Metastasis through a Novel Mechanism of Enhancing EGFR Signaling
Authors: Jiang Hong , Wu Lisha , Chen Jing , Mishra Murli , Chawsheen Hedy A. , Zhu Haining , Wei Qiou
Published: April 2023

Ectopical expression of FlagSrx in HT29 cells promotes the C-terminal tyrosine phosphorylation of EGFR and activates downstream MAPK signaling. Cells were serum starved for overnight and then treated with or without 50 ng/ml of EGF containing medium for indicated times. Results from a single membrane western blot were shown in (A) and quantitative data from three independent experiments were shown in (B). Compared to control cells, * p< 0.05 (paired t test, n = 3).
Table S9 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Analysis of the metabolites found and missed in FFPE human prostate sample categorized by superclass, class, subclass, substituent, physical/chemical properties, and pathways.
Supplementary Figure S4 from Sulfiredoxin Promotes Colorectal Cancer Cell Invasion and Metastasis through a Novel Mechanism of Enhancing EGFR Signaling
Authors: Jiang Hong , Wu Lisha , Chen Jing , Mishra Murli , Chawsheen Hedy A. , Zhu Haining , Wei Qiou
Published: April 2023

Knockdown of Srx in RKO cells reduces the C-terminal tyrosine phosphorylation of EGFR and inhibits downstream MAPK signaling. Cells were serum starved for overnight and then treated with or without 50 ng/ml of EGF containing medium for indicated times. Results from a single membrane western blot were shown in (A) and quantitative data from three independent experiments were shown in (B). Compared to control cells, * p< 0.05 (paired t test, n = 3).
Table S8 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Patient clinical characteristics and FFPE sample features.
Table S6 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Metabolite score values of reliability.
Table S5 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Analysis of the metabolites found in FF and missed in FFPE cell sample categorized by superclass, class, subclass, substituent, physical/chemical properties, and pathways.
Table S4 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Analysis of the metabolites found and missed in FF cell samples categorized by superclass, class, subclass, substituent, physical/chemical properties, and pathways.
Table S15 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Correlation analysis between metabolite intensity and percentage of epithelial tissue.
Supplementary Table 1 from Novel Potent and Selective Inhibitors of p90 Ribosomal S6 Kinase Reveal the Heterogeneity of RSK Function in MAPK-Driven Cancers
Authors: Aronchik Ida , Appleton Brent A. , Basham Stephen E. , Crawford Kenneth , Del Rosario Mercedita , Doyle Laura V. , Estacio William F. , Lan Jiong , Lindvall Mika K. , Luu Catherine A. , Ornelas Elizabeth , Venetsanakos Eleni , Shafer Cynthia M. , Jefferson Anne B.
Published: April 2023

XLSX file - 27K, Competition binding of LJH685, LJI308, BI-D1870-AE, and FMK-PA at 10 microM concentration to either 96 or 492 kinases.
Table S13 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Mean, standard deviation, and significance of the signal intensity ratio between taurine and cytidine 5'-diphosphocholine, and dihomo-linolenate and cytidine 5'-diphosphocholine acroos frozen and FFPE samples.
Table S12 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Ranking of the housekeeping metabolites.
Supplementary Figures 1 - 4 from Novel Potent and Selective Inhibitors of p90 Ribosomal S6 Kinase Reveal the Heterogeneity of RSK Function in MAPK-Driven Cancers
Authors: Aronchik Ida , Appleton Brent A. , Basham Stephen E. , Crawford Kenneth , Del Rosario Mercedita , Doyle Laura V. , Estacio William F. , Lan Jiong , Lindvall Mika K. , Luu Catherine A. , Ornelas Elizabeth , Venetsanakos Eleni , Shafer Cynthia M. , Jefferson Anne B.
Published: April 2023

PDF file - 650K, Figure S1. RSK N-terminal Kinase Directly Phosphorylates YB1 on Ser102. COS7 cells either (A) untransfected or (B) transiently expressing WT RSK2 or T493M RSK2 were placed under serum starvation conditions for 20 hrs, followed by 3 hour treatment with 1microM FMK-PA and subsequently stimulated with 1ng/ml EGF for 10 minutes. The levels of phosphorylated and total RSK2 and YB1 proteins were assessed in total cell lysates using Western Blot. β-actin protein levels were assessed for gel loading control. Figure S2. LJH685 modulates YB1 and BAD phosphorylation in either attached or anchorageindependent setting to similar extent and at similar concentrations of LJH685. MDA-MB-231 cells were treated with the indicated kinase inhibitors for 4 hours. Cell lysates were analyzed by Western blot with indicated antibodies. Figure S3. MDA-MB-231 cells were plated in Matrigel with DMSO or LJH685 for 6 days and colony morphology was examined using phase microscopy. Figure S4. MDA-MB-231 and H358 cells were plated on regular and ultra-low bind tissue culture plates for 5 days in presence of 10 microM LJH685 or DMSO. Cell Cycle distribution was assessed using FACS analysis of propidium iodide stained nuclei.
Table S10 from Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues
Authors: Cacciatore Stefano , Zadra Giorgia , Bango Clyde , Penney Kathryn L. , Tyekucheva Svitlana , Yanes Oscar , Loda Massimo
Published: April 2023

Analysis of the metabolites found and missed in FFPE cell and human prostate sample categorized by superclass, class, subclass, substituent, physical/chemical properties, and pathways.
Supplementary Table 7 from Gene Silencing Associated with SWI/SNF Complex Loss during NSCLC Development
Authors: Song Shujie , Walter Vonn , Karaca Mehmet , Li Ying , Bartlett Christopher S. , Smiraglia Dominic J. , Serber Daniel , Sproul Christopher D. , Plass Christoph , Zhang Jiren , Hayes D. Neil , Zheng Yanfang , Weissman Bernard E.
Published: April 2023

XLSX file - 71K, Genes upregulated by 5dAzaC or TSA.
Supplementary Table 6 from Gene Silencing Associated with SWI/SNF Complex Loss during NSCLC Development
Authors: Song Shujie , Walter Vonn , Karaca Mehmet , Li Ying , Bartlett Christopher S. , Smiraglia Dominic J. , Serber Daniel , Sproul Christopher D. , Plass Christoph , Zhang Jiren , Hayes D. Neil , Zheng Yanfang , Weissman Bernard E.
Published: April 2023

XLSX file - 63K, Genes upregulated by any treatment.
Supplementary Table 5 from Gene Silencing Associated with SWI/SNF Complex Loss during NSCLC Development
Authors: Song Shujie , Walter Vonn , Karaca Mehmet , Li Ying , Bartlett Christopher S. , Smiraglia Dominic J. , Serber Daniel , Sproul Christopher D. , Plass Christoph , Zhang Jiren , Hayes D. Neil , Zheng Yanfang , Weissman Bernard E.
Published: April 2023

XLSX file - 68K, Genes upregulated by BRG1 or TSA.
Supplementary Table 4 from Gene Silencing Associated with SWI/SNF Complex Loss during NSCLC Development
Authors: Song Shujie , Walter Vonn , Karaca Mehmet , Li Ying , Bartlett Christopher S. , Smiraglia Dominic J. , Serber Daniel , Sproul Christopher D. , Plass Christoph , Zhang Jiren , Hayes D. Neil , Zheng Yanfang , Weissman Bernard E.
Published: April 2023

XLSX file - 90K, Genes upregulated by BRG1 or 5dAzaC.
Supplementary Materials from PAI-1 Leads to G1-Phase Cell-Cycle Progression through Cyclin D3/cdk4/6 Upregulation
Authors: Giacoia Evan Gomes , Miyake Makito , Lawton Adrienne , Goodison Steve , Rosser Charles J.
Published: April 2023

PDF file - 87KB
Supplementary Table 1 from Gene Silencing Associated with SWI/SNF Complex Loss during NSCLC Development
Authors: Song Shujie , Walter Vonn , Karaca Mehmet , Li Ying , Bartlett Christopher S. , Smiraglia Dominic J. , Serber Daniel , Sproul Christopher D. , Plass Christoph , Zhang Jiren , Hayes D. Neil , Zheng Yanfang , Weissman Bernard E.
Published: April 2023

XLSX file - 623K, Genes that are upregulated or downregulated by BRG1.
Supplementary Figure 1 from Gene Silencing Associated with SWI/SNF Complex Loss during NSCLC Development
Authors: Song Shujie , Walter Vonn , Karaca Mehmet , Li Ying , Bartlett Christopher S. , Smiraglia Dominic J. , Serber Daniel , Sproul Christopher D. , Plass Christoph , Zhang Jiren , Hayes D. Neil , Zheng Yanfang , Weissman Bernard E.
Published: April 2023

PDF file - 4305K, Validation of gene expression for microarray and RGLS analyses; RGLS analysis of the A427 cell line.
Supplementary Figure 1 from PAI-1 Leads to G1-Phase Cell-Cycle Progression through Cyclin D3/cdk4/6 Upregulation
Authors: Giacoia Evan Gomes , Miyake Makito , Lawton Adrienne , Goodison Steve , Rosser Charles J.
Published: April 2023

PDF file - 704KB, PAI-1 overexpression in human cervical tumors.
Supplementary Materials and Methods from AKT-Induced Tamoxifen Resistance Is Overturned by RRM2 Inhibition
Authors: Shah Khyati N. , Mehta Kshama R. , Peterson David , Evangelista Marie , Livesey John C. , Faridi Jesika S.
Published: April 2023

PDF file - 135 KB
Supplementary Figure Legend from SGEF Is Regulated via TWEAK/Fn14/NF-κB Signaling and Promotes Survival by Modulation of the DNA Repair Response to Temozolomide
Authors: Ensign Shannon P. Fortin , Roos Alison , Mathews Ian T. , Dhruv Harshil D. , Tuncali Serdar , Sarkaria Jann N. , Symons Marc H. , Loftus Joseph C. , Berens Michael E. , Tran Nhan L.
Published: April 2023

Supplementary Figure Legend
Supplementary Figure 5 from SGEF Is Regulated via TWEAK/Fn14/NF-κB Signaling and Promotes Survival by Modulation of the DNA Repair Response to Temozolomide
Authors: Ensign Shannon P. Fortin , Roos Alison , Mathews Ian T. , Dhruv Harshil D. , Tuncali Serdar , Sarkaria Jann N. , Symons Marc H. , Loftus Joseph C. , Berens Michael E. , Tran Nhan L.
Published: April 2023

Supplementary Figure 5. SGEF is required for BRCA1 recruitment to H2A.X foci following TMZ treatment.
Supplementary Figures 1-5 from A Genome-Wide Loss-of-Function Screen Identifies SLC26A2 as a Novel Mediator of TRAIL Resistance
Authors: Dimberg Lina Y. , Towers Christina G. , Behbakht Kian , Hotz Taylor J. , Kim Jihye , Fosmire Susan , Porter Christopher C. , Tan Aik-Choon , Thorburn Andrew , Ford Heide L.
Published: April 2023

Supplemental Figure 1: TRAIL-resistant BJAB-LexR cells are not resistant to other drugs. Supplemental Figure 2: TRAIL-resistant MDA-MB-231 cells are not resistant to other drugs. Supplemental Figure 3: Knock-down of SLC26A2 does not affect sensitivity tovdoxorubicin and etoposide in TRAIL resistant MDA-MB-231. Supplemental Figure 4: BJAB-LexR cells have increased SLC26A2 and decreased DR4 and DR5. Supplemental Figure 5: SLC26A2 expression correlates with Metastasis prognosis.
Supplementary Figure 3 from SGEF Is Regulated via TWEAK/Fn14/NF-κB Signaling and Promotes Survival by Modulation of the DNA Repair Response to Temozolomide
Authors: Ensign Shannon P. Fortin , Roos Alison , Mathews Ian T. , Dhruv Harshil D. , Tuncali Serdar , Sarkaria Jann N. , Symons Marc H. , Loftus Joseph C. , Berens Michael E. , Tran Nhan L.
Published: April 2023

Supplementary Figure 3. SGEF depletion in glioma cells display impaired DNA double strain break repair kinetics.
Supplementary Figure 2 from SGEF Is Regulated via TWEAK/Fn14/NF-κB Signaling and Promotes Survival by Modulation of the DNA Repair Response to Temozolomide
Authors: Ensign Shannon P. Fortin , Roos Alison , Mathews Ian T. , Dhruv Harshil D. , Tuncali Serdar , Sarkaria Jann N. , Symons Marc H. , Loftus Joseph C. , Berens Michael E. , Tran Nhan L.
Published: April 2023

Supplementary Figure 2. SGEF knockdown enhances glioma cell susceptibility to TMZ-induced cell death.
Supplemental Legends from A Genome-Wide Loss-of-Function Screen Identifies SLC26A2 as a Novel Mediator of TRAIL Resistance
Authors: Dimberg Lina Y. , Towers Christina G. , Behbakht Kian , Hotz Taylor J. , Kim Jihye , Fosmire Susan , Porter Christopher C. , Tan Aik-Choon , Thorburn Andrew , Ford Heide L.
Published: April 2023

Supplemental Legends 1-5
Supplementary Table 2 from A Monotonic and Prognostic Genomic Signature from Fibroblasts for Colorectal Cancer Initiation, Progression, and Metastasis
Authors: Berdiel-Acer Mireia , Cuadras Daniel , Díaz-Maroto Natalia Guillén , Sanjuan Xavier , Serrano Teresa , Berenguer Antoni , Moreno Victor , Gonçalves-Ribeiro Samuel , Salazar Ramon , Villanueva Alberto , Molleví David G.
Published: April 2023

PDF file - 55KB, Public databases used in the study.
RT-PCR analysis of EGFR extracellular domain mutations in the remaining CBRC NB tumors from A Novel EGFR Extracellular Domain Mutant, EGFRΔ768, Possesses Distinct Biological and Biochemical Properties in Neuroblastoma
Authors: Keller James , Nimnual Anjaruwee S. , Varghese Mathew S. , VanHeyst Kristen A. , Hayman Michael J. , Chan Edward L.
Published: April 2023

S1. All PCR products were confirmed to be either EGFRÎ"768 or EGFRvIII by bidirectional sequencing. Gel purified EGFRvIII and EGFRÎ"768 cDNA were positive controls. H2O was negative control.
Data Supplement from Targeted Deletion and Lipidomic Analysis Identify Epithelial Cell COX-2 as a Major Driver of Chemically Induced Skin Cancer
Authors: Jiao Jing , Ishikawa Tomo-O , Dumlao Darren S. , Norris Paul C. , Magyar Clara E. , Mikulec Carol , Catapang Art , Dennis Edward A. , Fischer Susan M. , Herschman Harvey R.
Published: April 2023

Skin F4/80+ macrophage from Cox-2deltaM (Cox-2flox/flox;LysMCre+) mice undergo effective targeted Cox-2 gene deletion. Macrophages were isolated from the skin of 7-9 week old Cox-2deltaM and Cox-2fl/fl mice that were treated one time with TPA to induce COX-2 expression. Skin specimens were treated with 3 mg/ml Dispase for 16 hrs at 4oC; the epidermis was then separated from the dermis. The dermal layer was further digested, at 37 oC for 1.5 hrs, in RPMI1640 medium containing 1mg/ml collagenase I (Invitrogen, Carlsbad, CA). The dissociated cells were stained with PE-conjugated anti-F4/80 antibody, and F4/80+ cells were isolated by FACS. (A) FACS plots show, in red, the sorted F4/80+ populations from Cox-2deltaM and Cox-2fl/fl dissociated dermal layers. (B) DNAs from the isolated F4/80+ cell populations were extracted and the presence of the Cox-2fl, Cox-2del, and Cre alleles were examined by PCR. PCR analysis demonstrates the presence of both the Cre and the Cox-2del alleles in F4/80+ cells isolated from Cox-2deltaMmice. (C) Total RNA was isolated with an RNeasy Mini Kit (Qiagen, Valencia, CA), from sorted F4/80+ cell populations. RNAs were reverse transcribed into cDNA with SuperScript III First-Strand Synthesis System for qRT-PCR (Invitrogen, Carlsbad, CA), and quantitative PCR was done in the iQ thermal cycler (Bio-Rad) using the iQSYBR Green Supermix (Bio-Rad). Cox-2 forward primer: 5'-TGA TCGAAGACTACGTGCAA-3'; reverse primer: 5'-GTGAGTCCATGTTCCAGGAG-3'. The graph shows significantly decreased Cox-2 gene expression in F4/80+ Cox-2deltaM cells when compared to F4/80+ Cox-2fl/fl cells. β-Actin was used as the reference gene. Error bars are SD. * indicates p<0.05.
Figure S5 from Distinct Effects of Adipose-Derived Stem Cells and Adipocytes on Normal and Cancer Cell Hierarchy
Authors: Anjanappa Manjushree , Burnett Riesa , Zieger Michael A. , Merfeld-Clauss Stephanie , Wooden William , March Keith , Tholpady Sunil , Nakshatri Harikrishna
Published: April 2023

Prognostic value of HGF/IFNgamma ratio in breast cancer
Supplementary Figure S6 from Defining ATM-Independent Functions of the Mre11 Complex with a Novel Mouse Model
Authors: Balestrini Alessia , Nicolas Laura , Yang-lott Katherine , Guryanova Olga A. , Levine Ross L. , Bassing Craig H. , Chaudhuri Jayanta , Petrini John H.J.
Published: April 2023

Immunohistochemical characterization of thymic lymphomas.
SUPPLEMENTARY MATERIALS from Defining ATM-Independent Functions of the Mre11 Complex with a Novel Mouse Model
Authors: Balestrini Alessia , Nicolas Laura , Yang-lott Katherine , Guryanova Olga A. , Levine Ross L. , Bassing Craig H. , Chaudhuri Jayanta , Petrini John H.J.
Published: April 2023

EXTENDED EXPERIMENTAL PROCEDURES and SUPPLEMENTARY FIGURE LEGENDS
Supplementary Figure Legend from Actin-Binding Protein, Espin: A Novel Metastatic Regulator for Melanoma
Authors: Yanagishita Takeshi , Yajima Ichiro , Kumasaka Mayuko , Kawamoto Yoshiyuki , Tsuzuki Toyonori , Matsumoto Yoshinari , Watanabe Daisuke , Kato Masashi
Published: April 2023

PDF file - 89K
Data Supplement from Bioactive Lipids, LPC and LPA, Are Novel Prometastatic Factors and Their Tissue Levels Increase in Response to Radio/Chemotherapy
Authors: Schneider Gabriela , Sellers Zachariah Payne , Abdel-Latif Ahmed , Morris Andrew J. , Ratajczak Mariusz Z.
Published: April 2023

Supplementary Table 1. Sequences of primers employed for RT-PCR and qRT-PCR analyses.
Data Supplement from Bioactive Lipids, LPC and LPA, Are Novel Prometastatic Factors and Their Tissue Levels Increase in Response to Radio/Chemotherapy
Authors: Schneider Gabriela , Sellers Zachariah Payne , Abdel-Latif Ahmed , Morris Andrew J. , Ratajczak Mariusz Z.
Published: April 2023

Supplementary Figure 1. Wound healing assay indicates that LPA and LPC may induce chemokinesis of RMS cells. Panel A - Representative pictures taken during wound healing assay. Panel B - Statistical analysis of number of cells that migrate into wound.
Data Supplement from Bioactive Lipids, LPC and LPA, Are Novel Prometastatic Factors and Their Tissue Levels Increase in Response to Radio/Chemotherapy
Authors: Schneider Gabriela , Sellers Zachariah Payne , Abdel-Latif Ahmed , Morris Andrew J. , Ratajczak Mariusz Z.
Published: April 2023

Supplementary Figure 3. Ki16425 is not toxic against RMS cells. Panel E and F - Images of cells that were incubated in the absence (vehicle) or presence of Ki16425. Panel E - cells incubated with medium supplemented with 10% FBS, Panel F - cells incubated in medium supplemented with 0.5% BSA. Representative images are shown. Panel G - Chemotaxis to LPA of RH30 cells pretreated for 1h with 10 μM Ki16425 Results are presented as a percentage Ki16425. of cells migrating to LPA gradient as compared to control cells pretreated with vehicle only. Based on fact that chemotaxis assay was run for 24 hours, exposure of RMS cells to Ki16425 significantly affect responsiveness of RMS cells to LPA for at least 36 hours [24 hours (time of assay) + 12 hours (cells kept in culture before performing assay)]. The recovery of responsiveness to LPA gradient after 24 hours indicates transient but not toxic effect of Ki16425.
Supplementary Figure Legends from Stromal Senescence By Prolonged CDK4/6 Inhibition Potentiates Tumor Growth
Authors: Guan Xiangnan , LaPak Kyle M. , Hennessey Rebecca C. , Yu Christina Y. , Shakya Reena , Zhang Jianying , Burd Christin E.
Published: April 2023

Supplementary Figure Legends
Figure S4 from p120-Catenin Downregulation and PIK3CA Mutations Cooperate to Induce Invasion through MMP1 in HNSCC
Authors: Kidacki Michal , Lehman Heather L. , Green Michelle V. , Warrick Joshua I. , Stairs Douglas B.
Published: April 2023

Immunohistochemistry of EPC1 3D organotypic cultures. A, 3D cultures were stained for P120CTN and B, E-cadherin. Scale bars = 50μm. n=3.
Dataset S1 from Stromal Senescence By Prolonged CDK4/6 Inhibition Potentiates Tumor Growth
Authors: Guan Xiangnan , LaPak Kyle M. , Hennessey Rebecca C. , Yu Christina Y. , Shakya Reena , Zhang Jianying , Burd Christin E.
Published: April 2023

OpenArray® profiling results.
Supplementary Data from Quantitative Proteome Heterogeneity in Myeloproliferative Neoplasm Subtypes and Association with JAK2 Mutation Status
Authors: Socoro-Yuste Nuria , Čokić Vladan P. , Mondet Julie , Plo Isabelle , Mossuz Pascal
Published: April 2023

Table S1. Deregulated proteins between JAK2(+) PV and JAK2(-) ET/PMF. Table S2. Deregulated proteins between JAK2(+) ET and JAK2(-) ET/PMF. Table S3. Deregulated proteins between JAK2(+) PMF and JAK2(-) ET/PMF. Table S4. Deregulated proteins between JAK2(+) PV and JAK2(+) PMF. Table S5. Deregulated proteins between JAK2(+) ET and JAK2(+) PMF. Table S6. Deregulated proteins between JAK2(+) PV and JAK2(+) ET. Table S7. Deregulated pathways between JAK2(+) PV and JAK2(-) ET/PMF. Table S8. Deregulated pathways between JAK2(+) ET and JAK2(-) ET/PMF. Table S9. Deregulated pathways between JAK2(+) PMF and JAK2(-) ET/PMF. Table S10. Deregulated pathways between JAK2(+) PV and JAK2(+) PMF. Table S11. Deregulated pathways between JAK2(+) ET and JAK2(+) PMF. Table S12. Deregulated pathways between JAK2(+) PV and JAK2(+) ET.
Supplementary Table 4 from RNA-seq Reveals Aurora Kinase–Driven mTOR Pathway Activation in Patients with Sarcomatoid Metastatic Renal Cell Carcinoma
Authors: Pal Sumanta K. , He Miaoling , Tong Tommy , Wu Huiqing , Liu Xueli , Lau Clayton , Wang Jin-Hui , Warden Charles , Wu Xiwei , Signoretti Sabina , Choueiri Toni K. , Karam Jose A. , Jones Jeremy O.
Published: April 2023

Significantly different genes sarcomatoid vs. clear cell.
Data Supplement from Cyclosporin A Promotes Tumor Angiogenesis in a Calcineurin-Independent Manner by Increasing Mitochondrial Reactive Oxygen Species
Authors: Zhou Alice Yao , Ryeom Sandra
Published: April 2023

Supplemental Figure 1: Calcineurin inhibitors have opposing effects on tumorigenesis and endothelial cells.
Supplementary Table 2 from RNA-seq Reveals Aurora Kinase–Driven mTOR Pathway Activation in Patients with Sarcomatoid Metastatic Renal Cell Carcinoma
Authors: Pal Sumanta K. , He Miaoling , Tong Tommy , Wu Huiqing , Liu Xueli , Lau Clayton , Wang Jin-Hui , Warden Charles , Wu Xiwei , Signoretti Sabina , Choueiri Toni K. , Karam Jose A. , Jones Jeremy O.
Published: April 2023

Forward and reverse primers.
Video 3 from Therapeutic Targeting of Nuclear γ-Tubulin in RB1-Negative Tumors
Authors: Lindström Lisa , Villoutreix Bruno O. , Lehn Sophie , Hellsten Rebecka , Nilsson Elise , Crneta Enisa , Olsson Roger , Alvarado-Kristensson Maria
Published: April 2023

Video 3. Differential Interference Contrast (DIC)/fluorescence time-lapse microscopy images of a mitotic MCF10A treated with DMSO (vehicle). Hoechst 33258 was used to stain chromatin. Images were collected every 30 s.
Supplemental Material from The Cytoskeletal Adapter Protein Spinophilin Regulates Invadopodia Dynamics and Tumor Cell Invasion in Glioblastoma
Authors: Cheerathodi Mujeeburahiman , Avci Naze G. , Guerrero Paola A. , Tang Leung K. , Popp Julia , Morales John E. , Chen Zhihua , Carnero Amancio , Lang Frederick F. , Ballif Bryan A. , Rivera Gonzalo M. , McCarty Joseph H.
Published: April 2023

Supplementary Table 1. Peptides identified by LC-MS/MS and SEQUEST searching for integrin Î±v, integrin Î²8, and Spinophilin. Supplemental Figure 1. Analysis of Î²8 integrin-Spn binding and Spn protein localization in vitro and in cultured cells. Supplemental Figure 2. Spn protein is expressed in recurrent human GBM samples. Supplemental Figure 3. Spn mRNA is differentially expressed in GBM regions, but expression levels do not correlate with patient survival. Supplemental Figure 4. Experimental strategy to generate mosaic mouse models of the brain cancer astrocytoma. Supplemental Figure 5. Genetically targeting Spn in human GBM cells using RNAi leads to enhanced tumor cell invasion. Supplemental Figure 6. Microscopic analysis of Spn-dependent invasive growth in LN229 tumors. Supplemental Figure 7. Immunohistochemical analysis of xenograft tumors derived from LN229 cells expressing control or Spn shRNAs. Supplemental Figure 8. Analysis of invadopodia formation in GBM cells. Supplemental Videos 1 and 2. Imaging Rac1 GTPase activities in live cells by FRET.
Supplementary figure S5 from Therapeutic Targeting of Nuclear γ-Tubulin in RB1-Negative Tumors
Authors: Lindström Lisa , Villoutreix Bruno O. , Lehn Sophie , Hellsten Rebecka , Nilsson Elise , Crneta Enisa , Olsson Roger , Alvarado-Kristensson Maria
Published: April 2023

Supplementary figure S5. The effect of CDA on tumor growth of xenografted U1690 cells in the presence or absence of RB1 expression.
Supplementary Figure 7 from Mcl-1 Mediates TWEAK/Fn14-Induced Non–Small Cell Lung Cancer Survival and Therapeutic Response
Authors: Whitsett Timothy G. , Mathews Ian T. , Cardone Michael H. , Lena Ryan J. , Pierceall William E. , Bittner Michael , Sima Chao , LoBello Janine , Weiss Glen J. , Tran Nhan L.
Published: April 2023

PDF file - 53K, Pharmacologic inhibition of Mcl-1 abrogates TWEAK-mediated cell survival.
Supplementary figure S3 from Therapeutic Targeting of Nuclear γ-Tubulin in RB1-Negative Tumors
Authors: Lindström Lisa , Villoutreix Bruno O. , Lehn Sophie , Hellsten Rebecka , Nilsson Elise , Crneta Enisa , Olsson Roger , Alvarado-Kristensson Maria
Published: April 2023

Supplementary figure S3. Dimethyl fumarate interacts with the GTP/GDP binding pocket of ÃŽÂ³-tubulin.
Supplementary Figure 5 from Mcl-1 Mediates TWEAK/Fn14-Induced Non–Small Cell Lung Cancer Survival and Therapeutic Response
Authors: Whitsett Timothy G. , Mathews Ian T. , Cardone Michael H. , Lena Ryan J. , Pierceall William E. , Bittner Michael , Sima Chao , LoBello Janine , Weiss Glen J. , Tran Nhan L.
Published: April 2023

PDF file - 98K, Bcl-xL depletion sensitizes lung cancer cells to therapeutic insult but does not fully rescue TWEAK-induced cell survival.
Supplementary Figure 4 from Mcl-1 Mediates TWEAK/Fn14-Induced Non–Small Cell Lung Cancer Survival and Therapeutic Response
Authors: Whitsett Timothy G. , Mathews Ian T. , Cardone Michael H. , Lena Ryan J. , Pierceall William E. , Bittner Michael , Sima Chao , LoBello Janine , Weiss Glen J. , Tran Nhan L.
Published: April 2023

PDF file - 35K, TWEAK exposure enhances Mcl-1 and Bcl-xL mRNA expression.
Supplemental Figure Legend from HuR Contributes to TRAIL Resistance by Restricting Death Receptor 4 Expression in Pancreatic Cancer Cells
Authors: Romeo Carmella , Weber Matthew C. , Zarei Mahsa , DeCicco Danielle , Chand Saswati N. , Lobo Angie D. , Winter Jordan M. , Sawicki Janet A. , Sachs Jonathan N. , Meisner-Kober Nicole , Yeo Charles J. , Vadigepalli Rajanikanth , Tykocinski Mark L. , Brody Jonathan R.
Published: April 2023

Supplemental Figure Legend
Supplementary Figure 2 from mTOR Inhibition Potentiates HSP90 Inhibitor Activity via Cessation of HSP Synthesis
Authors: Acquaviva Jaime , He Suqin , Sang Jim , Smith Donald L. , Sequeira Manuel , Zhang Chaohua , Bates Richard C. , Proia David A.
Published: April 2023

PDF file - 624K, Kinetics of HSP70 gene regulation.
Video S6 from Dual Targeting of Mesenchymal and Amoeboid Motility Hinders Metastatic Behavior
Authors: Jones Brandon C. , Kelley Laura C. , Loskutov Yuriy V. , Marinak Kristina M. , Kozyreva Varvara K. , Smolkin Matthew B. , Pugacheva Elena N.
Published: April 2023

Ibidi chemotactic invasion of HCC1143 cells with NEDD9 depletion
Supplemental Figures 1-14 from Depleting Tumor-NQO1 Potentiates Anoikis and Inhibits Growth of NSCLC
Authors: Madajewski Brian , Boatman Michael A. , Chakrabarti Gaurab , Boothman David A. , Bey Erik A.
Published: April 2023

Supplemental Figure 1: Tumor-NQO1 overexpression leads to poor prognosis in lung cancer patients. Supplemental Figure 2: shNQO1-B knockdown of NQO1 leads to decreased growth in soft agar. Supplemental Figure 3: shNQO1-B knockdown of NQO1 in H292 cells leads to decreased growth in soft agar. Supplemental Figure 4: Dicoumarol inhibits growth of A549 cells in soft agar. Supplemental Figure 5: Mac220 inhibits growth of A549 cells in soft agar. Supplemental Figure 6: Stable overexpression of NQO1 in H596 (NQO1 null) lung cancer cells causes increased growth in soft agar. Supplemental Figure 7: Transient knockdown of NQO1 in H596 LPC-NQO1 cells causes decreased growth in soft agar. Supplemental Figure 8: shNQO1-B knockdown of NQO1 in A549 cells decreases cell invasion. Supplemental Figure 9: Stable overexpression of NQO1 in H596 NQO1 null cells causes increased invasion. Supplementary Figure 10: Transient knockdown of NQO1 using siRNA inhibits invasion of HCC1171 cells. Supplemental Figure 11: Stable knockdown of NQO1 in A549 cells causes loss of PARP- 1 protein expression. Supplemental Figure 12: Knockdown of NQO1 reduces ALDH (high) activity in Pancreas cancer cells. Supplemental Figure 13: Knockdown of NQO1 reduces ALDH (high) activity in Prostate cancer cells. Supplemental Figure 14: NQO1 knockdown does not inhibit short-term viability our long-term survival of non-transformed human bronchial epithelial cells.
Fig S1 from Exploiting AR-Regulated Drug Transport to Induce Sensitivity to the Survivin Inhibitor YM155
Authors: Nyquist Michael D. , Corella Alexandra , Burns John , Coleman Ilsa , Gao Shuai , Tharakan Robin , Riggan Luke , Cai Changmeng , Corey Eva , Nelson Peter S. , Mostaghel Elahe A.
Published: April 2023

Fig S1. Androgen Mediated Growth Suppression in LnCaP
Supplemental Excel File 6 from A New Role for ERα: Silencing via DNA Methylation of Basal, Stem Cell, and EMT Genes
Authors: Ariazi Eric A. , Taylor John C. , Black Michael A. , Nicolas Emmanuelle , Slifker Michael J. , Azzam Diana J. , Boyd Jeff
Published: April 2023

Supplemental Excel File 6 - Consensus ERalpha Inversely-related Genes
Data Supplement from PTEN Deficiency Mediates a Reciprocal Response to IGFI and mTOR Inhibition
Authors: Patel Mukund , Gomez Nicholas C. , McFadden Andrew W. , Moats-Staats Billie M. , Wu Sam , Rojas Andres , Sapp Travis , Simon Jeremy M. , Smith Scott V. , Kaiser-Rogers Kathleen , Davis Ian J.
Published: April 2023

Supplementary Figure Legends
Data Supplement from SMAD4 Suppresses AURKA-Induced Metastatic Phenotypes via Degradation of AURKA in a TGFβ-Independent Manner
Authors: Jia Lina , Lee Hun Seok , Wu Chun Fu , Kundu Juthika , Park Sang Gyu , Kim Ryong Nam , Wang Li-Hui , Erkin Özgür Cem , Choi Jong-Sun , Chae Seoung Wan , Yang Ho Bin , Choi Yoon-La , Shin Young Kee
Published: April 2023

Supplemental Figure Legend
Supplementary Figures from Abituzumab Targeting of αV-Class Integrins Inhibits Prostate Cancer Progression
Authors: Jiang Yuan , Dai Jinlu , Yao Zhi , Shelley Greg , Keller Evan T.
Published: April 2023

Supplemental Figure 1. Anti-Integrin alphaV antibody EPR16800 inhibits prostate cancer motility and invasiveness. Supplemental Figure 2. Anti-Integrin alphaV antibody EPR16800 inhibits expression of alphaV integrin and phosphorylation of FAK, Akt and ERK in LNCaP and C4-2B cells.
Supplementary Figure 3 from Loss of Keratinocytic RXRα Combined with Activated CDK4 or Oncogenic NRAS Generates UVB-Induced Melanomas via Loss of p53 and PTEN in the Tumor Microenvironment
Authors: Coleman Daniel J. , Chagani Sharmeen , Hyter Stephen , Sherman Anna M. , Löhr Christiane V. , Liang Xiaobo , Ganguli-Indra Gitali , Indra Arup K.
Published: April 2023

Supplementary Figure S3: Controls for IHC experiments used in this study. (A) For all IHC studies, a liquid-repellant slide marker pen was used to separate out a single section on each slide that does not receive primary antibody but otherwise carried through the same labeling protocol; for use as a negative control to demonstrate that signals observed were specific. (B) PEP1 antibody (anti-TYRP1) and PEPS antibody (anti-TYRP2) give similar specific staining patterns for melanocytic cells in RxraL2/L2 neonatal skin following irradiation with a single UVB dose. (C) Fluorescent IHC for p-AKT and PTEN on chronically-irradiated TAN skin showing similar staining pattern to chromogenic IHC shown in Figure 5. (D,E) Non-UVB treated skin showed reduced staining intensity of PTEN, p-AKT, and p53 compared to irradiated skin. Unlike skin treated with chronic UVB, no discernable difference in expression pattern between RxraL2/L2 and bi enic Rxraep-/- mice was observed. E=E idermis, D=Dermis, scale bars =1 00 J.Jm.
Supplementary Methods, Figures 1 - 3, Tables 1 - 2 from DNA Damage-Binding Complex Recruits HDAC1 to Repress Bcl-2 Transcription in Human Ovarian Cancer Cells
Authors: Zhao Ran , Han Chunhua , Eisenhauer Eric , Kroger John , Zhao Weiqiang , Yu Jianhua , Selvendiran Karuppaiyah , Liu Xingluo , Wani Altaf A. , Wang Qi-En
Published: April 2023

PDF file - 440K, Supplementary Figure 1. DDB2 expression is inversely correlated with Bcl-2 expression in human ovarian cancer cells and tissues. Supplementary Figure 2. Recruitment of transcription factor E2F1 and Sp1 to the bcl-2 promoter. Supplementary Figure 3: Purification of DDB1, DDB2 and GST-HDAC1. Table S1:Antibodies used for ChIP, IP and Immunoblotting. Table S2: Primers for ChIP and RT-PCR.
Supplementary Figure 2 from Active FOXO1 Is a Key Determinant of Isoform-Specific Progesterone Receptor Transactivation and Senescence Programming
Authors: Diep Caroline H. , Knutson Todd P. , Lange Carol A.
Published: April 2023

Differential transcriptional regulation of PR isoform-specific target genes in ovarian and breast cancer cells.
Supplementary Figure 1 from Active FOXO1 Is a Key Determinant of Isoform-Specific Progesterone Receptor Transactivation and Senescence Programming
Authors: Diep Caroline H. , Knutson Todd P. , Lange Carol A.
Published: April 2023

Gene Ontology (GO) analysis of significantly regulated genes in PR-expressing ovarian cancer cells.
Supplemental Data from Oncofetal Chondroitin Sulfate Glycosaminoglycans Are Key Players in Integrin Signaling and Tumor Cell Motility
Authors: Clausen Thomas Mandel , Pereira Marina Ayres , Al Nakouzi Nader , Oo Htoo Zarni , Agerbæk Mette Ø , Lee Sherry , Ørum-Madsen Maj Sofie , Kristensen Anders Riis , El-Naggar Amal , Grandgenett Paul M. , Grem Jean L. , Hollingsworth Michael A. , Holst Peter J. , Theander Thor , Sorensen Poul H. , Daugaard Mads , Salanti Ali
Published: April 2023

Figure legends for the supplemental figures and supplemental table 1
Supplementary Materials and Methods from FOXO3a and Posttranslational Modifications Mediate Glucocorticoid Sensitivity in B-ALL
Authors: Consolaro Francesca , Ghaem-Maghami Sadaf , Bortolozzi Roberta , Zona Stefania , Khongkow Mattaka , Basso Giuseppe , Viola Giampietro , Lam Eric W.-F.
Published: April 2023

Supplementary Materials and Methods
Supplementary Figure S5 from FOXO3a and Posttranslational Modifications Mediate Glucocorticoid Sensitivity in B-ALL
Authors: Consolaro Francesca , Ghaem-Maghami Sadaf , Bortolozzi Roberta , Zona Stefania , Khongkow Mattaka , Basso Giuseppe , Viola Giampietro , Lam Eric W.-F.
Published: April 2023

Protein expression of P-FOXO3a (S7) was analyzed at baseline conditions in five poor responder patients (PPR) and 9 glucocorticoid good responder patients (PGR) by western blot analysis. Equal amounts of protein were loaded. The first 10 patients are as in Fig. 1.
Supplementary Figure S3 from FOXO3a and Posttranslational Modifications Mediate Glucocorticoid Sensitivity in B-ALL
Authors: Consolaro Francesca , Ghaem-Maghami Sadaf , Bortolozzi Roberta , Zona Stefania , Khongkow Mattaka , Basso Giuseppe , Viola Giampietro , Lam Eric W.-F.
Published: April 2023

Sirtinol but not EX527 or PDF-170 significantly enhances ithe cytotoxic and cytostatic function of dexamethasone in B-ALL cell lines REH and RS4;11 cells were treated with the a range of concentrations of SIRT inhibitors (0-100μM) and/or dexamethasone. Cell viability analysis was performed after 24, 48 and 72 h using MTT assay. A) Sintinol (SIRT1/2/6 inhibitor); B) EX527 (SIRT1 inhibitor); C) PDF-170 (SIRT2 inhibitor). (t-test: Sirtinol with 1μM dexamethasone verses dexamethasone at 1μM) *significant p<0.05, ** very significant p<0.01 and no marker: not significant.
Table S1 from S1P Stimulates Proliferation by Upregulating CTGF Expression through S1PR2-Mediated YAP Activation
Authors: Cheng Jung-Chien , Wang Evan Y. , Yi Yuyin , Thakur Avinash , Tsai Shu-Huei , Hoodless Pamela A.
Published: April 2023

Primers for RT-qPCR
Supplementary Figure S1 from FOXO3a and Posttranslational Modifications Mediate Glucocorticoid Sensitivity in B-ALL
Authors: Consolaro Francesca , Ghaem-Maghami Sadaf , Bortolozzi Roberta , Zona Stefania , Khongkow Mattaka , Basso Giuseppe , Viola Giampietro , Lam Eric W.-F.
Published: April 2023

JNK, p38 and ERK inhibitors do not substantially inhibit the cytotoxic and cytostatic function of dexamethasone in B-ALL cell lines REH and RS4;11 cells were treated with the a range of concentrations of (0-100μM) of JNK, p38 and ERK inhibitors, SP600125, SB202190 and PD98059 and/or dexamethasone. Cell viability analysis was performed after 48 h using MTT assay.
Figure S3 from S1P Stimulates Proliferation by Upregulating CTGF Expression through S1PR2-Mediated YAP Activation
Authors: Cheng Jung-Chien , Wang Evan Y. , Yi Yuyin , Thakur Avinash , Tsai Shu-Huei , Hoodless Pamela A.
Published: April 2023

S FIG. 3. HNF4α negatively regulates S1P-induced up-regulation of Ctgf in mouse HCC cells.
Supplementary Figure Legends from FOXO3a and Posttranslational Modifications Mediate Glucocorticoid Sensitivity in B-ALL
Authors: Consolaro Francesca , Ghaem-Maghami Sadaf , Bortolozzi Roberta , Zona Stefania , Khongkow Mattaka , Basso Giuseppe , Viola Giampietro , Lam Eric W.-F.
Published: April 2023

Supplementary Figure Legends
Supplemental Data from Differential Regulation of ZEB1 and EMT by MAPK-Interacting Protein Kinases (MNK) and eIF4E in Pancreatic Cancer
Authors: Kumar Krishan , Chow Christina R. , Ebine Kazumi , Arslan Ahmet D. , Kwok Benjamin , Bentrem David J. , Eckerdt Frank D. , Platanias Leonidas C. , Munshi Hidayatullah G.
Published: April 2023

Supplemental data related to Figs. 2 and 5 S1. Collagen-dependent MNK phosphorylation in chemoresistant PDAC cells. S2. Targeting eIF4E increases ZEB1 levels in AsPC1 and Panc1 cells. S3. eIF4E and MNK1/2 do not regulate expression of primary transcript of miR-200 (pri-miR-200) microRNAs A and B. S4. miR-141 and miR-200c regulate ZEB1 levels in CD18-CR cells A. S5. Targeting the MNK effector hnRNPA1 increases ZEB1 protein without increasing ZEB1 mRNA levels.
Supplementary Data from LIN28B Activation by PRL-3 Promotes Leukemogenesis and a Stem Cell–like Transcriptional Program in AML
Authors: Zhou Jianbiao , Chan Zit-Liang , Bi Chonglei , Lu Xiao , Chong Phyllis S.Y. , Chooi Jing-Yuan , Cheong Lip-Lee , Liu Shaw-Cheng , Ching Ying Qing , Zhou Yafeng , Osato Motomi , Tan Tuan Zea , Ng Chin Hin , Ng Siok-Bian , Wang Shi , Zeng Qi , Chng Wee-Joo
Published: April 2023

This file contains additional Methods and Results. S1. Real-time quantitative RT-PCR (qRT-PCR) validation of expression levels of some selected genes from microarray study (n = 3, mean {plus minus}SD). S2. Functional networks were generated by MetaCore program for the analysis of the genes differentially expressed 2 fold and above in TF1-hPRL3 vs TF1-pEGFP. S3. FACS analysis of CD34+CD38- populations in AML cell lines (A), AML primary bone marrow cells (B) and healthy control bone marrow cells (C). S4. Characterization of TF1-FUGW and TF1-FUGW-hPRL3 cell lines. S5. Histological characterization of diseased mice in serial dilution experiments (in vivo). S6. (A) Western blotting analysis of PRL-3 and LIN28B proteins in TF1 cells transfected with either pEGFP, wild type hPRL3 or catalytically inactive mutant hPRL3 (C104S). S7. Functional evaluation of let-7 miRNAs in MOLM-14 cells. Table S1. The full gene list of differentially expressed above 2 fold in TF1-hPRL3 cells vs TF1-pEGFP cells. Table S2. A list of 10 most upregulated and downregulated genes identified from comparison of TF1-hPRL-3 and TF1-pEGFP cells by microarray analysis. Table S3. In vivo limiting dilution analysis of TF1-hPRL3 cells. Table S4. Summary of LIN28B staining distribution with IHC method in bone marrow samples from patients with AML at diagnosis. Table S5. Comparison of clinical and molecular characteristics of 108 AML patients according to LIN28B expression levels. Table S6. Univariate and Multivariate Analysis of Disease-Free Survival in AML patients with LIN28B High or LIN28B Low Groups.
Supplementary Figures 1- 4 from miR-19–Mediated Inhibition of Transglutaminase-2 Leads to Enhanced Invasion and Metastasis in Colorectal Cancer
Authors: Cellura D. , Pickard K. , Quaratino S. , Parker H. , Strefford J.C. , Thomas G.J. , Mitter R. , Mirnezami A.H. , Peake N.J.
Published: April 2023

Supplementary Figures 1- 4. Supplementary figure 1: Differences in TG2 expression between SW480 and SW620 cells. Supplementary figure 2: Pro-inflammatory cytokine expression in SW480/SW620 cells. Supplementary figure 3: MMP expression in SW480/SW620 cells. Supplementary figure 4: regulation of TG2 by overexpression of miRNA-19 in SW480/SW620 cells.
Supplementary Material from EPAC–RAP1 Axis-Mediated Switch in the Response of Primary and Metastatic Melanoma to Cyclic AMP
Authors: Rodríguez Carlos I. , Castro-Pérez Edgardo , Prabhakar Kirthana , Block Laura , Longley B. Jack , Wisinski Jaclyn A. , Kimple Michelle E. , Setaluri Vijayasaradhi
Published: April 2023

Fig. S1. Characterization of melanoma tumors and tumor derived cells of BrafCA/Pten-/- mouse. Fig. S2. Status of cAMP signaling in metastatic melanoma. Fig S3. Effect of cAMP inducing agent on human metastatic melanoma cell lines. Fig. S4. Survival of metastatic melanoma cells to MAPK pathway inhibition and elevation of [cAMP]. Fig. S5. Effect of cAMP elevation in AKT and mTOR signaling in human melanoma cells. Fig. S6. Effect of PKA inhibition by H89 on sensitivity of melanoma cells to BRAF(V600E) inhibitor PLX4032. Fig. S7. Effect of CREB knockdown and cAMP elevation in human metastatic melanoma cells. Fig. S8. Expression and the role of EPAC and RAP1 in human melanoma cells. Fig. S9. Role of EPAC in melanoma survival and MAPK pathway inhibition. Fig. S10. Effect of EPAC inhibition on MITF and pigmentation related proteins in human melanoma cells. Fig. S11. RAP1B overexpression in human melanoma cells.
Supplementary figure legends from RAD51 and BRCA2 Enhance Oncolytic Adenovirus Type 5 Activity in Ovarian Cancer
Authors: Tookman Laura A. , Browne Ashley K. , Connell Claire M. , Bridge Gemma , Ingemarsdotter Carin K. , Dowson Suzanne , Shibata Atsushi , Lockley Michelle , Martin Sarah A. , McNeish Iain A.
Published: April 2023

Legends for supplementary figures