bluuebunny/crossref_metadata_2025 huggingface dataset:
Immune, Oxidative, and Morphological Changes in the Livers of Tibetan Sheep after Feeding Resveratrol and β-Hydroxy-β-methyl Butyric Acid: A Transcriptome–Metabolome Integrative Analysis
Authors: Chen Xuan , Zhang Fengshuo , Raza Sayed Haidar Abbas , Wu Zhenling , Su Quyangangmao , Ji Qiurong , He Tingli , Zhu Kaina , Zhang Yu , Hou Shengzhen , Gui Linsheng
Published: September 2024

This study investigated the effects of dietary resveratrol (RES) and β-Hydroxy-β-methyl butyric acid (HMB) on immune, oxidative, and morphological changes in the livers of Tibetan sheep using transcriptomics and metabolomics. One hundred and twenty male Tibetan lambs of a similar initial weight (15.5 ± 0.14 kg) were randomly divided into four groups with thirty lambs per treatment: (1) H group (basal diet without RES or HMB); (2) H-RES group (1.5 g/day of RES); (3) H-HMB group (1250 mg/day of HMB); (4) H-RES-HMB group (1.5 g/day of RES and 1250 mg/day of HMB). The experiment was conducted for 100 days, including a pre-test period of 10 days and a formal period of 90 days. The results showed significantly increased concentrations of glutathione peroxidase, superoxide dismutase, and IgM in the H-RES-HMB group (p < 0.05), while the malondialdehyde levels were significantly decreased (p < 0.05). The glycolytic indices including creatinine kinase (CK), malate dehydrogenase (MDH), and succinate dehydrogenase (SDH) were significantly increased in the H-RES-HMB group compared with the others (p < 0.05). A histological analysis showed that the hepatic plate tissue in the H-RES-HMB group appeared normal with multiple cells. The transcriptomic analysis showed that the expression of genes associated with the calcium signaling pathway (MYLK2, CYSLTR2, ADCY1, HRH1, ATP2B2, NOS2, HRC, ITPR1, and CAMK2B) and the NF-κB signaling pathway (BCL2 and CARD14) in the H-RES-HMB group were upregulated. The key differential metabolites (d-pyroglutamic acid, DL-serine, DL-threonine, fumarate, and glyceric acid) were enriched in the pathways associated with D-amino acid metabolism, the citrate cycle (TCA cycle), and carbon metabolism. The combined transcriptomic and non-targeted metabolomic analyses showed the co-enrichment of differential genes (NOS2 and GLUD1) and metabolites (fumarate) in arginine biosynthesis-regulated glycolytic activity, whereas the differential genes (ME1, SCD5, FABP2, RXRG, and CPT1B) and metabolites (Leukotriene b4) co-enriched in the PPAR signaling pathway affected the immune response by regulating the PI3K/AKT and cGMP/PKG signaling. In conclusion, the dietary RES and HMB affected the hepatic antioxidant capacity, immune response, and glycolytic activity through modulating the transcriptome (BCL2, CAMK2B, ITPR1, and IL1R1) and metabolome (DL-serine, DL-threonine, fumaric acid, and glycolic acid).
Recycling of Sewage Sludge: Synthesis and Application of Sludge-Based Activated Carbon in the Efficient Removal of Cadmium (II) and Lead (II) from Wastewater
Authors: Aljubiri Salha M. , Younes Ayman A. O. , Alosaimi Eid H. , Abdel Daiem Mahmoud M. , Abdel-Salam Enas T. , El-Shwiniy Walaa H.
Published: September 2024

The limited supply of drinking water has aroused people’s curiosity in recent decades. Adsorption is a popular method for removing hazardous substances from wastewater, especially heavy metals, as it is cheap, highly efficient, and easy to use. In this work, a new sludge-based activated carbon adsorbent (thickened samples SBAC1 and un-thickened samples SBAC2) was developed to remove hazardous metals such as cadmium (Cd+2) and lead (Pb+2) from an aqueous solution. The chemical structure and surface morphology of the produced SBAC1 and SBAC2 were investigated using a range of analytical tools such as CHNS, BET, FT-IR, XRD, XRF, SEM, TEM, N2 adsorption/desorption isothermal, and zeta potential. BET surface areas were examined and SBAC2 was found to have a larger BET surface area (498.386 m2/g) than SBAC1 (336.339 m2/g). While the average pore size was 10–100 nm for SBAC1 and 45–50 nm for SBAC2. SBAC1 and SBAC2 eliminated approximately 99.99% of Cd+2 and Pb+2 out the water under all conditions tested. The results of the adsorption of Cd+2 and Pb+2 were in good agreement with the pseudo-second-order equation (R2 = 1.00). Under the experimental conditions, the Cd+2 and Pb+2 adsorption equilibrium data were effectively linked to the Langmuir and Freundlich equations for SBAC1 and SBAC2, respectively. The regeneration showed a high recyclability for the fabricated SBAC1 and SBAC2 during five consecutive reuse cycles. As a result, the produced SBAC1 and SBAC2 are attractive adsorbents for the elimination of heavy metals from various environmental and industrial wastewater samples.
The Role of Mutated Calreticulin in the Pathogenesis of BCR-ABL1-Negative Myeloproliferative Neoplasms
Authors: Vadeikienė Roberta , Jakštys Baltramiejus , Laukaitienė Danguolė , Šatkauskas Saulius , Juozaitytė Elona , Ugenskienė Rasa
Published: September 2024

Myeloproliferative neoplasms (MPNs) are characterized by increased proliferation of myeloid lineages in the bone marrow. Calreticulin (CALR) 52 bp deletion and CALR 5 bp insertion have been identified in essential thrombocythemia (ET) and primary myelofibrosis (PMF). There is not much data on the crosstalk between mutated CALR and MPN-related signaling pathways, such as JAK/STAT, PI3K/Akt/mTOR, and Hedgehog. Calreticulin, a multifunctional protein, takes part in many cellular processes. Nevertheless, there is little data on how mutated CALR affects the oxidative stress response and oxidative stress-induced DNA damage, apoptosis, and cell cycle progression. We aimed to investigate the role of the CALR 52 bp deletion and 5 bp insertion in the pathogenesis of MPN, including signaling pathway activation and functional analysis in CALR-mutated cells. Our data indicate that the JAK/STAT and PI3K/Akt/mTOR pathways are activated in CALR-mutated cells, and this activation does not necessarily depend on the CALR and MPL interaction. Moreover, it was found that CALR mutations impair calreticulin function, leading to reduced responses to oxidative stress and DNA damage. It was revealed that the accumulation of G2/M-CALR-mutated cells indicates that oxidative stress-induced DNA damage is difficult to repair. Taken together, this study contributes to a deeper understanding of the specific molecular mechanisms underlying CALR-mutated MPNs.
Mass Spectrometry Advancements and Applications for Biomarker Discovery, Diagnostic Innovations, and Personalized Medicine
Authors: Son Ahrum , Kim Woojin , Park Jongham , Park Yongho , Lee Wonseok , Lee Sangwoon , Kim Hyunsoo
Published: September 2024

Mass spectrometry (MS) has revolutionized clinical chemistry, offering unparalleled capabilities for biomolecule analysis. This review explores the growing significance of mass spectrometry (MS), particularly when coupled with liquid chromatography (LC), in identifying disease biomarkers and quantifying biomolecules for diagnostic and prognostic purposes. The unique advantages of MS in accurately identifying and quantifying diverse molecules have positioned it as a cornerstone in personalized-medicine advancement. MS-based technologies have transformed precision medicine, enabling a comprehensive understanding of disease mechanisms and patient-specific treatment responses. LC-MS has shown exceptional utility in analyzing complex biological matrices, while high-resolution MS has expanded analytical capabilities, allowing the detection of low-abundance molecules and the elucidation of complex biological pathways. The integration of MS with other techniques, such as ion mobility spectrometry, has opened new avenues for biomarker discovery and validation. As we progress toward precision medicine, MS-based technologies will be crucial in addressing the challenges of individualized patient care, driving innovations in disease diagnosis, prognosis, and treatment strategies.
QYOLO: Contextual Query-Assisted Object Detection in High-Resolution Images
Authors: Gao Mingyang , Wang Wenrui , Mao Jia , Xiong Jun , Wang Zhenming , Wu Bo
Published: September 2024

High-resolution imagery captured by drones can detect critical components on high-voltage transmission towers, providing inspection personnel with essential maintenance insights and improving the efficiency of power line inspections. The high-resolution imagery is particularly effective in enhancing the detection of fine details such as screws. The QYOLO algorithm, an enhancement of YOLOv8, incorporates context queries into the feature pyramid, effectively capturing long-range dependencies and improving the network’s ability to detect objects. To address the increased network depth and computational load introduced by query extraction, Ghost Separable Convolution (GSConv) is employed, reducing the computational expense by half and further improving the detection performance for small objects such as screws. The experimental validation using the Transmission Line Accessories Dataset (TLAD) developed for this project demonstrates that the proposed improvements increase the average precision (AP) for small objects by 5.5% and the F1-score by 3.5%. The method also enhances detection performance for overall targets, confirming its efficacy in practical applications.
Pruning Policy for Image Classification Problems Based on Deep Learning
Authors: Pachon Cesar G. , Pinzon-Arenas Javier O. , Ballesteros Dora
Published: September 2024

In recent years, several methods have emerged for compressing image classification models using CNNs, for example, by applying pruning to the convolutional layers of the network. Typically, each pruning method uses a type of pruning distribution that is not necessarily the most appropriate for a given classification problem. Therefore, this paper proposes a methodology to select the best pruning policy (method + pruning distribution) for a specific classification problem and global pruning rate to obtain the best performance of the compressed model. This methodology was applied to several image datasets to show the influence not only of the method but also of the pruning distribution on the quality of the pruned model. It was shown that the selected pruning policy affects the performance of the pruned model to different extents, and that it depends on the classification problem to be addressed. For example, while for the Date Fruit Dataset, variations of more than 10% were obtained, for CIFAR10, variations were less than 5% for the same cases evaluated.
Effects of Global Climate Warming on the Biological Characteristics of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)
Authors: Fu Chun , Liu Zhiqian , Xu Danping , Peng Yaqin , Liu Biyu , Zhuo Zhihang
Published: September 2024

Spodoptera frugiperda (J.E. Smith) is a significant economic pest that has recently invaded Africa and Asia. However, much of the information regarding its ecological capabilities in these newly invaded environments remains largely unknown. In this study, the life history traits of the fall armyworm under conditions of increased temperature, different photoperiods, and varying humidity levels were systematically evaluated. Among 43 studies, a total of 20 studies were included in the analysis by passing the screening criteria, and random-effects meta-analysis, fixed-effects meta-analysis, and meta-regression were conducted. It has been found that with the increase in temperature above 20 °C, various physiological indicators of the fall armyworm are significantly enhanced. When the temperature reaches 32 °C, the physiological activities of S. frugiperda are at their highest point. As the temperature increases, the duration of each developmental stage of the fall armyworm decreases significantly, accompanied by an increase in oviposition quantity and period in females. Additionally, the pupal development time is shortened, which leads to an increase in the lifespan of the adult moth. Using temperature and relative humidity as environmental variables, the optimal survival conditions for each insect state of the fall armyworm were calculated. These findings can assist in predicting the population dynamics of the fall armyworm and in formulating appropriate and practical management strategies.
The Belowground–Aboveground Interactions of Zucchini: The Effects of Trichoderma afroharzianum Strain T22 on the Population and Behavior of the Aphid Aphis gossypii Glover and Its Endoparasitoid Aphidius colemani Viereck
Authors: Battaglia Donatella , Mang Stefania Mirela , Caccavo Vittoria , Fanti Paolo , Forlano Pierluigi
Published: September 2024

Fungi belonging to the genus Trichoderma have received high consideration in agriculture due to their beneficial effects on crops from their plant promotion effects and protection from disease. A role of Trichoderma fungi in triggering plant defense mechanisms against insect pests, either directly or by natural enemy attraction, has been proposed, even if the results in different studies are controversial. In this present study, using zucchini plants as a model species, we investigated the effects of Trichoderma afroharzianum strain T22 plant inoculation on the cotton aphid Aphis gossypii and its endoparasitoid Aphidius colemani. Our results showed that the inoculation with T. afroharzianum T22 promotes A. gossypii population growth and makes zucchini more attractive to the aphid. The higher abundance of aphids on Trichoderma-inoculated zucchini was compensated for by a higher presence of the mummies of Aphidius colemani. In this present study, we recorded a higher zucchini biomass, thereby confirming that Trichoderma can act as a plant growth inducer.
Evaluating the Efficacy of Plant Extracts in Managing the Bruchid Beetle, Callosobruchus maculatus (Coleoptera: Bruchidae)
Authors: Akbar Rasheed , Faheem Brekhna , Aziz Tariq , Ali Amjad , Ullah Asmat , Khan Imtiaz Ali , Sun Jianfan
Published: September 2024

An estimated 2000 plant species have been employed for pest control worldwide. The use of these botanical derivatives is thought to be one of the most cost-effective and sustainable options for pest management in stored grain. The present study was designed to assess the efficacy of five plant extracts viz; Nicotiana tabacum L., Nicotiana rustica L., Azadirachta indica A. Juss., Thuja orientalis L., and Melia azedarach L. against Callosobruchus maculatus L. Plant species extracts were applied at six different concentrations, i.e., 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0% in four replications. The phytochemical analyses of ethanolic extracts of five plant species showed variable amounts of phytochemicals i.e., alkaloids, flavonoids, saponins, diterpenes, phytosterol, and phenols. Total phenolic and flavonoid compounds were also observed. The efficacy of A. indica was highest, characterized by the lowest infestation rate (16.65%), host seed weight loss (7.85%), mean oviposition (84.54), and adult emergence (58.40%). In contrast, T. orientalis was found to be the least effective against C. maculatus, with the highest infestation rate of 25.60%, host seed weight loss of 26.73%, mean oviposition of 117.17, and adult emergence rate of 82.01%. Probit analysis was performed by estimating LC50 and LC90. The toxicity percentages of N. tabacum (LC50 = 0.69%, LC90 = 14.59%), N. rustica (LC50 = 0.98%, LC90 = 22.06%), and A. indica (LC50 = 1.09%, LC90 = 68.52%) were notable in terms of the lower LC50 and LC90 values after the 96-h exposure period against C. maculatus. Repellency was assessed by using the area preference and filter paper method. The repellency of C. maculatus on plant extracts increased with the increasing dose and time, such that it was the highest after 48 h. Likewise, at a 3% concentration, A. indica demonstrated 100.00% (Class-V) repellency followed by N. tabacum (96.00%, Class-V), N. rustica (74%, Class-IV), M. azedarach (70.00%, Class-IV), and T. orientalis (68.00%, Class-IV). Based on the findings of this study, we recommend integrating N. rustica, N. tabacum, A. indica, and M. azedarach for effective management of C. maculatus and highlight the potential of these plant species in the formulation of new biocidal agents.
Extended Lichtenstein Repair for an Additional Femoral Canal Hernia
Authors: De Gols Johan , Berkmans Evelien , Timmers Mieke , Vanluyten Cedric , Ceulemans Laurens J. , Deferm Nathalie P.
Published: September 2024

The Lichtenstein procedure is one of the most performed surgeries worldwide. However, proper examination to exclude a femoral hernia is often not performed, resulting in a high number of missed hernias. For patients in whom a femoral hernia is suspected pre- or intraoperatively, we describe a novel surgical technique of a femoral extension to the classic Lichtenstein repair. We aim to investigate its safety and clinical outcome. Methods: The femoral–extended Lichtenstein is applied when a femoral hernia is suspected. The fascia transversalis is opened, the lacunar ligament incised, and the hernia reduced. A self-gripping mesh covers the femoral orifice equally on all sides. In a prospective single-center study, we compared 50 consecutive femoral–extended to 50 classic Lichtenstein repairs, evaluating operative time, patient-reported pain (intensity, duration), and recurrence. Results: The technique seems feasible and safe. Apart from 3 min additional surgical time, no difference in pain scoring or hernia recurrence was observed between both groups. Conclusions: We successfully introduced a femoral-extended Lichtenstein repair for patients with suspected femoral herniation.
Myoinositol and Metformin in the Prevention of Gestational Diabetes in High-Risk Patients: A Narrative Review
Authors: Romeo Paola , D’Anna Rosario , Corrado Francesco
Published: September 2024

Our hypothesis is that myoinositol and metformin in pregnant women with high-risk factors for glucose intolerance would reduce insulin resistance and consequently lower the incidence of gestational diabetes mellitus (GDM), a metabolic disorder of pregnancy characterized by maternal hyperglycemia due to deficient response to physiological insulin resistance, which may have a negative impact on perinatal outcome and long-term sequelae. In recent years, this pathology has become increasingly important given the global obesity epidemic and the delay in becoming pregnant, especially in industrialized countries. For this reason, the attempt to prevent, rather than cure, gestational diabetes is particularly important. In addition to lifestyle changes (especially diet and doing more exercise), myoinositol and metformin are the most promising factors at the moment, although not all RCTs published so far agree on their real effectiveness. A review of the articles published so far allows us to assume, albeit with some distinctions, that they can play a positive role.
Clinical Characteristics of Punctate Hyperfluorescence Spots in the Fellow Eye of Patients with Unilateral Macular Neovascularization with No Drusen
Authors: Kamao Hiroyuki , Goto Katsutoshi , Date Yuto , Hiraki Ryutaro , Mizukawa Kenichi , Miki Atsushi
Published: September 2024

Objectives: To assess the clinical characteristics of patients with macular neovascularization (MNV) with no drusen in the fellow eye, we investigated the incidence of MNV in fellow eyes and the outcomes of intravitreal aflibercept (IVA) monotherapy in MNV eyes of patients with unilateral MNV with a punctate hyperfluorescence spot (PHS) in the fellow eye. Methods: We retrospectively studied 58 treatment-naïve patients with unilateral MNV with no drusen in the fellow eye. Patients were classified into a PHS group (n = 29) or no-PHS group (n = 29) based on the presence of PHS. We evaluated the incidence of MNV in the fellow eye, and the retreatment rate after initiation of three monthly aflibercept injections over one year. Results: Fellow eyes in the PHS group had a thicker choroid (p < 0.05) and higher prevalence of pachychoroid pigment epitheliopathy (PPE) (p < 0.001). MNV eyes in the PHS group had a thicker choroid (p = 0.09). The PHS group had a lower retreatment rate (p < 0.05) and required fewer injections (p < 0.05) than the no-PHS group. MNV developed in one eye in both the PHS and no-PHS groups, and both cases occurred in areas of hypofluorescence on indocyanine green angiography within the PPE area before the onset of MNV. Conclusions: The PHS group frequently exhibited pachychoroid disease characteristics and responded better to IVA monotherapy than the no-PHS group. These groups may represent distinct populations of patients with unilateral MNV with no drusen in the fellow eye.
Health-Related Quality of Life with Six Domains: A Comparison of Healthcare Providers without Chronic Diseases and Participants with Chronic Diseases
Authors: Bujang Mohamad Adam , Hon Yoon Khee , Lai Wei Hong , Yap Eileen Pin Pin , Tiong Xun Ting , Ratnasingam Selvasingam , Kim Alex Ren Jye , Husin Masliyana , Jee Yvonne Yih Huan , Ahmad Nurul Fatma Diyana , Chew Cheng Hoon , Tan Clare Hui Hong , Khoo Sing Yee , Johari Fazalena , Fong Alan Yean Yip
Published: September 2024

Background/Objectives: This study aims to compare the health-related quality of life (HRQOL) between healthcare providers without chronic diseases and participants with chronic diseases presenting with one of the four different primary diagnoses on the health-related quality of life with six domains (HRQ-6D) scale. Methods: This is a cross-sectional study to compare the HRQOL between healthcare providers without chronic diseases and participants with chronic diseases. Data collection was performed from May 2022 to May 2023. Data for the comparison group were taken from healthcare providers without chronic diseases, and for the participant group with chronic diseases, the data were collected from actual patients with one of four types of primary diagnoses who were recruited from specialist cardiology, oncology, psychiatry, and nephrology clinics. All the participants of this study filled in the HRQ-6D. Results: There were 238 (58.6%) healthcare providers without chronic diseases who participated in this study, as well as 41 (10.1%) patients with end-stage renal disease (ESRD), 48 (11.8%) patients with cancer, and 40 (9.9%) patients who were depressed, and the remaining patients had heart disease. The means (SD) of HRQ-6D scores among healthcare providers without chronic diseases for pain, physical strength, emotion, mobility, self-care, perception of future health, and overall HRQ-6D score were 75.3% (19.8), 74.5% (21.1), 85.6% (18.4%), 93.0% (12.3), 91.6% (13.9), 74.2% (23.3), and 82.4% (13.6), respectively. In comparisons between healthcare providers without chronic diseases and participants with chronic diseases, all mean differences of the overall HRQ-6D score and its domains and dimensions were statistically significant (p < 0.001). Conclusions: The overall score of the HRQ-6D, as well as its domains and dimensions are sensitive in detecting the study participants with chronic diseases from among those without chronic diseases. Therefore, the HRQ-6D is a reliable and valid scale to measure HRQOL. Future studies may use this scale for interventional, observational, and cost-effectiveness studies.
Synergistic Effect in MoS2 Nanosheets–Biochar Nanocomposites with Enhanced Surface Area and Electrical Conductivity for Energy Storage Applications
Authors: Pandiselvi Thangaraj , Praveena Chithiraiselvan , Sridevi Venkatachalam , Venmathi Maran Balu Alagar , Kimura Masanari
Published: September 2024

Layered molybdenum disulfide (MoS2), a transition metal dichalcogenide, shows distinct optical, electrical, and physical properties at a few-layer thickness. MoS2 nanosheets (NSs) widely explored for energy and environmental applications but have limitations with respect to their electrical conductivity and charge transfer characteristics due to their low surface area. These limitations can be overcome by combining MoS2 NSs with carbon-based materials like graphene, carbon nanotubes, and biochar, which can enhance the properties in a synergistic way. In this study, biochar (BC), a carbon-rich material prepared from vegetable biomass through low-temperature pyrolysis has been combined with bulk MoS2 in various ratios using an aqueous phase exfoliation method to form MoS2 NSs–biochar nanocomposites. The spectroscopic, structural, and morphological studies confirmed the synergistic interaction between MoS2 and BC, which is well reflected in the facile exfoliation process and the formation of few layered MoS2 NSs on the surface of the BC without any agglomeration. The electrochemical studies prove that incorporating biochar into MoS2 enhances the capacitive behavior and reduces the charge transfer resistance compared to pristine MoS2 NSs and pristine biochar. This study provides ample scope for the composite to be explored for energy storage applications, especially towards the development of electrode materials due to the synergistic effect between MoS2 NSs and biochar.
Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
Authors: Moura Joana A. , Barlow Hugh J. , Doak Shareen H. , Hawkins Karl , Muller Iris , Clift Martin J. D.
Published: September 2024

A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the capacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells, the migration area of spheroids formed with the HULEC cell line was measured. The cells were first allowed to form a spheroid using the hanging drop technique before being encapsulated in the fibrin gel. The cells’ migration area was then measured after two days of embedding in the fibrin gel. Various conditions affecting fibrin gel polymerization, such as different concentrations of fibrinogen and thrombin, were evaluated alongside rheology, porosity, and fiber thickness analysis to understand how these factors influenced cell behavior within the composite biomaterial. Data point toward thrombin’s role in governing fibrin gel polymerization; higher concentrations result in less rigid gels (loss tangent between 0.07 and 0.034) and increased cell migration (maximum concentration tested: 5 U/mL). The herein presented method allows for a more precise determination of the crosslinking conditions of fibrin gel that can be used to stimulate angiogenic sprouting.
Bioprospecting of Mangrove Filamentous Fungi for the Biodegradation of Polyethylene Microplastics
Authors: Aguiar Arthur , Gama Letícia , Fornari Milene , Neto Almir , de Souza Rodrigo , Perna Rafael , Castro Laura , Kovacs Stella , Simões Marta Filipa , Ferreira Nelson , Domínguez Yoannis , de Castro Leandro , Ottoni Cristiane
Published: September 2024

The accumulation of microplastics (MPs) in the environment has been a bottleneck for scientific society. Several approaches have been described as possibilities for reducing MPs in aquatic and terrestrial ecosystems; however, most of them are not environmentally friendly. Filamentous fungi (Ff) cells are currently considered a promising solution as a treatment for MPs. Therefore, the present study reports the potential ability of Ff isolated from mangrove sediments to biodegrade low-density polyethylene MPs (LDPEMPs). Six Ff strains were grown in batch cultures for 28 days, and one of them, Aspergillus sp. (AQ3A), showed the most prominent profile to biodegrade polymeric compounds. After morphological and molecular analysis, all strains were identified as belonging to the genera Aspergillus (MQ1C, AQ2A and AQ3A), Penicillium (MQ1A), and Trichoderma (MQ1B and MQ2A). The strain Aspergillus sp. (AQ3A) showed the most promising results with a LDPEMPs reduction rate of 47% and biomass formation of 0.0890 g·mL−1. Complementary studies with Aspergillus sp. (AQ3A) using Fourier-transform infrared spectroscopy (FTIR) highlighted changes in the molecular structure of LDPEMPs. These results indicate that Ff can contribute to the biodegradation of LDPEMPs. However, other parameters, mainly associated with the enzymes that are involved in this biodegradation process, need to be explored.
Journal of Pharmaceutical and BioTech Industry: A New Open Access Journal
Authors: Bilgili Ecevit
Published: September 2024

The pharmaceutical and biotechnology industry continues to be one of the most important industry sectors for various reasons [...]
Prevalence of Online Political Incivility: Mediation Effects of Cognitive and Affective Involvement
Authors: Javeed Anam , Khan Muhammad Yar , Alomair Abdulrahman , Al Naim Abdulaziz S.
Published: September 2024

The aim of this study is to explore the impact of political news posts, interactive discussion factors, and uncivil comments on participants’ online incivility. The moderating effects of cognitive and affective involvement have also been considered. The data have been gathered using self-administered questionnaires from 458 respondents. The results indicate that political news posts having interactive discussion factors positively impacts the online uncivil behaviour of the participants. The uncivil comments initiated by other users also significantly impact the participants’ incivility. The data have also been analysed for the mediation effects. The construct of affective involvement emerged as an effective mediator as compared to cognitive involvement. This study posits several theoretical implications for the literature. The combination of the variables used is also rare in the literature. The findings of this study are useful for behavioural scientists, as the outcomes have a potential to predict the online behaviour of Pakistani users.
Acoustic Analysis of Vowels in Australian Aboriginal English Spoken in Victoria
Authors: Loakes Debbie , Gregory Adele
Published: September 2024

(1) Background: Australian Aboriginal English (AAE) is a variety known to differ in various ways from the mainstream, but to date very little phonetic analysis has been carried out. This study is a description of L1 Aboriginal English in southern Australia, aiming to comprehensively describe the acoustics of vowels, focusing in particular on vowels known to be undergoing change in Mainstream Australian English. Previous work has focused on static measures of F1/F2, and here we expand on this by adding duration analyses, as well as dynamic F1/F2 measures. (2) Methods: This paper uses acoustic-phonetic analyses to describe the vowels produced by speakers of Aboriginal Australian English from two communities in southern Australia (Mildura and Warrnambool). The focus is vowels undergoing change in the mainstream variety–the short vowels in KIT, DRESS, TRAP, STRUT, LOT, and the long vowel GOOSE; focusing on duration, and static and dynamic F1/F2. As part of this description, we analyse the data using the sociophonetic variables gender, region, and age, and also compare the Aboriginal Australian English vowels to those of Mainstream Australian English. (3) Results: On the whole, for duration, few sociophonetic differences were observed. For static F1/F2, we saw that L1 Aboriginal English vowel spaces tend to be similar to Mainstream Australian English but can be analysed as more conservative (having undergone less change) as has also been observed for L2 Aboriginal English, in particular for KIT, DRESS, and TRAP. The Aboriginal English speakers had a less peripheral vowel space than Mainstream Australian English speakers. Dynamic analyses also highlighted dialectal differences between Aboriginal and Mainstream Australian English speakers, with greater F1/F2 movement in the trajectories of vowels overall for AAE speakers, which was more evident for some vowels (TRAP, STRUT, LOT, and GOOSE). Regional differences in vowel quality between the two locations were minimal, and more evident in the dynamic analyses. (4) Conclusions: This paper further highlights how Aboriginal Australian English is uniquely different from Mainstream Australian English with respect to certain vowel differences, and it also highlights some ways in which the varieties align. The differences, i.e., a more compressed vowel space, and greater F1/F2 movement in the trajectories of short vowels for AAE speakers, are specific ways that Aboriginal Australian English and Mainstream Australian English accents are different in these communities in the southern Australian state of Victoria.
Gresilient Supplier Evaluation and Selection under Uncertainty Using a Novel Streamlined Full Consistency Method
Authors: Hashemi-Tabatabaei Mohammad , Amiri Maghsoud , Keshavarz-Ghorabaee Mehdi
Published: September 2024

Background: Supply chain management (SCM) plays a fundamental role in the progress and success of organizations and has continuously evolved to better adapt to today’s complex business environments. Consequently, the issue of supplier evaluation and selection (SES), which is one of the most critical decisions in SCM, has gained special significance and has been examined from various perspectives. The concept of green and resilient (gresilient) SCM has emerged in response to recent concerns about environmentally friendly production and operations, as well as organizations’ ability to cope with crises and disasters. In the rapidly growing construction industry, applying gresilient principles can ensure green operations and help overcome future challenges. Methods: This study focuses on gresilient SES in a real-world construction case study, proposing a streamlined FUCOM (S-FUCOM) approach. The proposed method streamlines traditional FUCOM processes to solve decision-making problems in deterministic and uncertain environments. Several numerical examples are provided to illustrate its applicability. Results: the case study results identify air emissions, environmental management systems, and restorative capacity as the most critical gresilient SES criteria. Conclusions: The third supplier emerged as the top performer based on decision-making indicators. Finally, a sensitivity analysis was conducted across 20 scenarios, demonstrating that S-FUCOM is robust and provides stable results.
T-Cells Rich Classical Hodgkin Lymphoma, a Pathology Diagnostic Pitfall for Nodular Lymphocyte-Predominant Hodgkin Lymphoma; Case Series and Review
Authors: Al-Maghrabi Haneen , Mokhtar Ghadeer , Noorsaeed Ahmed
Published: September 2024

Background: Some cases of classic Hodgkin lymphoma (CHL) display similarities to nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) in terms of architecture, leading to potential challenges in diagnosis. However, these difficulties can be overcome by conducting a thorough set of immunohistochemical examinations. Objective: To examine cases of T-cell-rich CHL that closely resemble the diagnosis of NLPHL, specifically pattern D, which can pose challenges in accurately determining the diagnosis even after conducting a thorough immunophenotypic assessment. Materials and methods: Histopathology slides of three cases of T-cell-rich CHL were retrieved and thoroughly examined to assess their clinical, immunomorphologic, and molecular features. Results: We present three cases containing cells that resembled lymphocyte predominant and Hodgkin Reed–Sternberg cells, expressing some B-cell antigens and CHL markers but all were lacking Epstein–Barr virus-encoded small RNA. All three cases were found in a background rich in T-cells with focal remaining follicular dendritic cell meshwork in one case. Only one case had few eosinophils while the other two had no background of eosinophils and plasma cells. Two patients presented with stage IIA and B-symptoms presented in one of them. Two patients were treated with four and six cycles of ABVD (doxorubicin, bleomycin, vinblastine, and dacarbazine), respectively. One patient planned to be treated with four cycles of ABVD plus Rituximab therapy. Conclusions: Some cases of Reed–Sternberg cells can show expression of both B-cell and CHL markers. This overlapping characteristic, which has not been extensively discussed in the existing literature, presents a unique challenge for treatment. Further research into these neoplasms may reveal valuable diagnostic and therapeutic implications.
Mechanism of Rock Mass Detachment Using Undercutting Anchors: A Numerical Finite Element Method (FEM) Analysis
Authors: Wójcik Andrzej , Jonak Kamil , Karpiński Robert , Jonak Józef , Kalita Marek , Prostański Dariusz
Published: September 2024

Undercutting anchors are structural elements used in construction and geotechnics to stabilize both structures and soils. Their main applications include stabilizing slopes and embankments, reinforcing foundations, and providing support during tunnel construction and other underground works. The authors propose the use of these anchors in rock mass detachment technology. This article presents a comprehensive analysis of the mechanism behind rock mass detachment using an undercutting anchor. Particular attention is given to the influence of parameters such as the fracture energy of the medium and the coefficient of friction between the medium and the anchor head on the detachment process of rock elements during anchor expansion in the drilled hole. Numerical FEM analysis was employed to model the effect of changes in the shape and size of failure cones under varying simulation conditions. The discussed problem is crucial for evaluating the effectiveness of this anchor design under non-standard conditions, particularly in the unconventional destruction of rock media.
Theory and Measurement of Heat Transport in Solids: How Rigidity and Spectral Properties Govern Behavior
Authors: Hofmeister Anne M.
Published: September 2024

Models of heat transport in solids, being based on idealized elastic collisions of gas molecules, are flawed because heat and mass diffuse independently in solids but together in gas. To better understand heat transfer, an analytical, theoretical approach is combined with data from laser flash analysis, which is the most accurate method available. Dimensional analysis of Fourier’s heat equation shows that thermal diffusivity (D) depends on length-scale, which has been confirmed experimentally for metallic, semiconducting, and electrically insulating solids. A radiative diffusion model reproduces measured thermal conductivity (K = DρcP = D × density × specific heat) for thick solids from ~0 to >1200 K using idealized spectra represented by 2–4 parameters. Heat diffusion at laboratory temperatures (conduction) proceeds by absorption and re-emission of infrared light, which explains why heat flows into, through, and out of a material. Because heat added to matter performs work, thermal expansivity is proportional to ρcP/Young’s modulus (i.e., rigidity or strength), which is confirmed experimentally over wide temperature ranges. Greater uptake of applied heat (e.g., cP generally increasing with T or at certain phase transitions) reduces the amount of heat that can flow through the solid, but because K = DρcP, the rate (D) must decrease to compensate. Laser flash analysis data confirm this proposal. Transport properties thus depend on heat uptake, which is controlled by the interaction of light with the material under the conditions of interest. This new finding supports a radiative diffusion mechanism for heat transport and explains behavior from ~0 K to above melting.
Diverse Strategies to Develop Poly(ethylene glycol)–Polyester Thermogels for Modulating the Release of Antibodies
Authors: Lipowska-Kur Daria , Otulakowski Łukasz , Szeluga Urszula , Jelonek Katarzyna , Utrata-Wesołek Alicja
Published: September 2024

In this work, we present basic research on developing thermogel carriers containing high amounts of model antibody immunoglobulin G (IgG) with potential use as injectable molecules. The quantities of IgG loaded into the gel were varied to evaluate the possibility of tuning the dose release. The gel materials were based on blends of thermoresponsive and degradable ABA-type block copolymers composed of poly(lactide-co-glycolide)-b-poly(ethylene glycol)-b-poly(lactide-co-glycolide) (PLGA–PEG–PLGA) or poly(lactide-co-caprolactone)-b-poly(ethylene glycol)-b-(lactide-co-caprolactone) (PLCL–PEG–PLCL). Primarily, the gels with various amounts of IgG were obtained via thermogelation, where the only factor inducing gel formation was the change in temperature. Next, to control the gels’ mechanical properties, degradation rate, and the extent of antibody release, we have tested two approaches. The first one involved the synergistic physical and chemical crosslinking of the copolymers. To achieve this, the hydroxyl groups located at the ends of the PLGA–PEG–PLGA chain were modified into acrylate groups. In this case, the thermogelation was accompanied by chemical crosslinking through the Michael addition reaction. Such an approach increased the dynamic mechanical properties of the gels and simultaneously prolonged their decomposition time. An alternative solution was to suspend crosslinked PEG–polyester nanoparticles loaded with IgG in a PLGA–PEG–PLGA gelling copolymer. We observed that loading IgG into thermogels lowered the gelation temperature (TGEL) value and increased the storage modulus of the gels, as compared with gels without IgG. The prepared gel materials were able to release the IgG from 8 up to 80 days, depending on the gel formulation and on the amount of loaded IgG. The results revealed that additional, chemical crosslinking of the thermogels and also suspension of particles in the polymer matrix substantially extended the duration of IgG release. With proper matching of the gel composition, environmental conditions, and the type and amount of active substances, antibody-containing thermogels can serve as effective IgG delivery materials.
Influence of Zinc Chloride Exposure on Microstructure and Mechanical Behavior of Age-Hardened AZ91 Magnesium Alloy
Authors: Konopík Pavel , Bucki Tomasz , Rzepa Sylwia , Melzer Daniel , Bolibruchová Dana , Li Ying , Džugan Jan
Published: September 2024

The AZ91 magnesium alloy was subjected to a complex treatment involving age hardening (supersaturation and artificial aging) and simultaneous surface layer modification. The specimens were supersaturated in contact with a mixture containing varying concentrations of zinc chloride, followed by cooling either in air or water. After supersaturation, the specimens were subjected to artificial aging and then air-cooled. This process resulted in the formation of a surface layer made of zinc-rich phases. The thickness and microstructure of the surface layer were influenced by the process parameters, namely, the zinc chloride content in the mixture and the cooling rate during supersaturation. The treated specimens exhibited favorable tensile strength and greater elongation compared to the as-cast AZ91 alloy, with values comparable to those of the alloy subjected to standard T6 tempering. No cracking of the layer was observed under moderate deformation, though greater deformation resulted in the formation of cracks, primarily in the areas containing the Mg5Al2Zn2 intermetallic phase. The produced layer demonstrated strong metallurgical bonding to the AZ91 substrate.
Low-Frequency Sound-Insulation Performance of Labyrinth-Type Helmholtz and Thin-Film Compound Acoustic Metamaterial
Authors: Hu Peizhou , Zhao Jingbo , Liu Hong , Zhang Xiaosheng , Zhang Guangjun , Yao Hong
Published: September 2024

This paper presents a type of acoustic metamaterial that combines a labyrinth channel with a Helmholtz cavity and a thin film. The labyrinth-opening design and thin-film combination contribute to the metamaterial’s exceptional sound-insulation performance. After comprehensive research, it is observed that in the frequency range of 20–1200 Hz, this acoustic metamaterial exhibits multiple sound-insulation peaks, showing a high overall sound-insulation quality. Specifically, the first sound-insulation peak is 26.3 Hz, with a bandwidth of 13 Hz and giving a transmission loss of 56.5 dB, showing excellent low-frequency sound-insulation performance. To further understand the low-frequency sound-insulation mechanism, this paper uses the equivalent model method to conduct an acoustic–electrical analogy, construct an equivalent model of the acoustic metamaterial, and delve into the sound-insulation mechanism at the first sound-insulation peak. To confirm the validity of the theoretical calculations, physical experiments are carried out by 3D printing experimental samples. The analysis of the experimental data has yielded results that are consistent with the simulation data, providing empirical evidence for the accuracy of the theoretical model. The material has significant practical application value. Finally, various factors are studied in depth based on the established equivalent model, which can provide valuable insights for the design and practical engineering application of acoustic metamaterials.
Theoretical Study of the Multiferroic Properties of Pure and Ion-Doped Pb5M3F19, M = Fe, Cr, Al
Authors: Apostolova Iliana N. , Apostolov Angel T. , Wesselinowa Julia M.
Published: September 2024

In a first theoretical investigation of the multiferroic properties of Pb5Fe3F19 (PFF) and Pb5Cr3F19 (PCF), we analyze their magnetic, ferroelectric, and dielectric characteristics as functions of temperature, magnetic field, and ion doping concentration using a microscopic model and Green’s function theory. The temperature-dependent polarization in PFF and PCF shows a distinctive kink at the magnetic Neel temperature TN, which vanishes when an external magnetic field is applied, indicating the multiferroic behavior of these two compounds. Ion doping effectively tunes the properties of PFF and PCF. In PFF, Cr ion doping leads to a decrease in the Neel temperature TN, while Cr and Al ion doping lowers the ferroelectric Curie temperature TC. In the case of PCF, we observe the enhancement of TC by Fe ion doping and the reduction by Al ion doping. The last result coincides well quantitatively with the experimental data. Additionally, the magnetodielectric coefficient of PFF is enhanced with the increasing magnetic field.
Distribution of Rare Earth Elements in Ash from Lignite Combustion in Polish Power Plants
Authors: Adamczyk Zdzisław , Komorek Joanna , Kokowska-Pawłowska Magdalena , Nowak Jacek
Published: September 2024

Rare earth elements are an essential critical raw material in the development of modern technologies and are highly sensitive to both supply chain disruptions and market turbulence. The presented study examines the characteristics of fuel, fly ash, and bottom ash from lignite combustion in power plant units. Also, we attempted to determine the amount of amorphous glass in the ashes and whether and to what extent the glass from the ash samples is bound to REY. The suitability of the ash was assessed as an alternative source of REY. The fuel and ash samples were acquired from power plants in Poland. The tests determined the fuel quality parameters, including the chemical and phase composition, of amorphous glass using ICP-MS and XRD methods, respectively. The study showed that all ash samples dissolved in 4% HF were enriched in REY. The efficiency of REY enrichment varied, and its presence in the residue samples was found to be in similar proportions compared to the raw sample. All ash residue samples were enriched in critical elements. The obtained values of the Coutl prospective coefficient allowed for the classification of some of the analyzed ashes and their residues after dissolution in 4% HF as prospective REY raw materials.