bluuebunny/crossref_metadata_2025 huggingface dataset:
Construction of the CRISPR/Cas9 module and genetic transformation of aromatic rice cv. Mentik Wangi for developing bacterial leaf blight resistance
Authors: RIFHANI NURUL FARIDAH , APRIANA ANIVERSARI , SISHARMINI ATMITRI , SANTOSO TRI JOKO , TRIJATMIKO KURNIAWAN RUDI , SLAMET-LOEDIN INEZ H. , YUNUS AHMAD
Published: July 2023

Abstract. Rifhani NF, Apriana A, Sisharmini A, Santoso TJ, Trijatmiko KR, Slamet-Loedin IH, Yunus A. 2023. Construction of the CRISPR/Cas9 module and genetic transformation of aromatic rice cv. Mentik Wangi for developing bacterial leaf blight resistance. Biodiversitas 24: 3258-3268.Rice cv. Mentik Wangi is a local aromatic rice that is susceptible to pests and diseases, such as bacterial leaf blight (BLB) caused by Xanthomonas oryzae(Xoo). This bacteria can cause damage to plants thereby reducing crop yields. This study aimed to obtain the CRISPR/Cas9 module construct as well as introducing the construct into rice cv. Mentik Wangi for developing BLB resistance. The manufacture of the CRISPR/Cas9 module was carried out using the Golden Gate method, and the introduction of that construct into rice cv. Mentik Wangi was carried out using Agrobacterium tumefaciens. Constructing a CRISPR/Cas9 module with multiple gRNA of OsSWEET11 and OsSWEET14 genes has succeeded, 129 putative transformant lines of T0 generation produced with the regeneration and transformation efficiency values ??are respectively 9.4% and 9.8%. The results showed that 36 lines were positive for the hpt gene, indicating that the CRISPR/Cas9-gRNAOsSWEET module construct was successfully entered into rice cv. Mentik Wangi. Further analysis is needed to identify mutagenesis in the target gene region of TI generation transgenic plants as well as phenotypic tests for BLB resistance.
Is women’s household decision-making autonomy associated with their higher dietary diversity in Bangladesh? Evidence from nationally representative survey
Authors: Shourove Jahid Hasan , Meem Fariha Chowdhury , Rahman Mustafizur , Islam G. M. Rabiul
Published: July 2023

In Bangladesh, a low-quality repetitive diet characterized by starchy staple foods is typical, leading to disorders associated with micronutrient deficiencies, particularly among mothers and their children. The purpose of the study was to validate the link between women’s decision-making autonomy and higher dietary diversity score. Participants were ever married women aged 15–49 years old with comprehensive dietary information (n = 17,842), selected from the Bangladesh Demographic and Health Survey, 2014. The dietary diversity score (DDS) was obtained from a 24-h recall of dietary intake from nine food groups, categorized into lower DDS (DDS ≤ 4) and higher DDS (DDS ≥ 5). Descriptive analysis, bivariate and multivariate logistic regression were conducted using STATA version 15. Almost all women consumed starchy foods, flesh (83.86%), and fruits (67.30%). Using logistic regression, the odds of achieving dietary diversity score were higher among women who participated in household purchases (OR 2.40; 95% CI: 1.52–3.83; p = 0.022). Women who had higher and secondary education were 2.72 (95% CI: 1.49–3.02; p = 0.025) and 1.31 (95% CI: 0.58–2.18; p = 0.029) times more likely to achieve higher DDS than women having no education, as well as women in the richest quintile (OR 6.49; 95% CI: 4.12–8.5; p = 0.037) compared to women in the lowest quintile. This study highlighted the association of several socioeconomic conditions of ever married women and their dietary diversity score in Bangladesh. Therefore, promoting the women’s education status, improving the socioeconomic conditions, and prioritizing their decisions are recommended for the attainment of higher dietary diversity score.
Some Elements in The Fruit: An Assessment of Gingerbread Plum from Sokoto, Nigeria
Authors: Imam Aminu Umar , Shehu Sambo Umar , Sarkingobir Yusuf , Miya Yusuf Yahaya , Adili Shafiu Ismaila , Shehu Abubakar , Adamu Abdullahi Alhaji
Published: July 2023

A region like Sokoto that is been challenged by series of problems such as double burden of diseases, malnutrition, and food insecurity should utilized the available fruits for their inherent nutrient’s compositions. Therefore, the objective of this study was to assess the proximate and elemental contents (sodium, phosphorus, potassium, calcium, and magnesium) in gingerbread plum (Neocarya macrophylla) fruit which are essential for normal growth and development of body tissues for the human health. Neocarya macrophylla fruit is screened for certain nutritionally needed elements and proximate compositions using standard methods. Results obtained show that, crude protein with (2.70 ± 0.0057%) has the lowest concentration, the moisture content was 5.83 ± 0.57 percent, ash content was 5.33 ± 1.54 percent, crude lipid was 5.23 ± 0.15 percent, and crude fiber was 13.33 ± 4.48 percent. The determined levels of mineral elements (calcium, magnesium, phosphorus, sodium, and potassium) reveal 30.25000 ppm, 4.9001 ppm, 90. 1000 ppm, 40.563 ppm, and 67.5601 ppm, respectively. Therefore, the plant is nutritious and vital for metabolic processes.
Impact of cross border reverse migration in Delhi–UP region of India during COVID-19 lockdown
Authors: Dwivedi Shubhangi , Perumal Saravana Keerthana , Kumar Sumit , Bhattacharyya Samit , Kumari Nitu
Published: July 2023

The declaration of a nationwide lockdown in India led to millions of migrant workers, particularly from Uttar Pradesh (UP) and Bihar, returning to their home states without proper transportation and social distancing from cities such as Delhi, Mumbai, and Hyderabad. This unforeseen migration and social mixing accelerated the transmission of diseases across the country. To analyze the impact of reverse migration on disease progression, we have developed a disease transmission model for the neighboring Indian states of Delhi and UP. The model’s essential mathematical properties, including positivity, boundedness, equilibrium points (EPs), and their linear stability, as well as computation of the basic reproduction number

(

R

0

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\left({R}_{0})

, are studied. The mathematical analysis reveals that the model with active reverse migration cannot reach a disease-free equilibrium, indicating that the failure of restrictive mobility intervention caused by reverse migration kept the disease propagation alive. Further, PRCC analysis highlights the need for effective home isolation, better disease detection techniques, and medical interventions to curb the spread. The study estimates a significantly shorter doubling time for exponential growth of the disease in both regions. In addition, the occurrence of synchronous patterns between epidemic trajectories of the Delhi and UP regions accentuates the severe implications of migrant plight on UP’s already fragile rural health infrastructure. By using COVID-19 incidence data, we quantify key epidemiological parameters, and our scenario analyses demonstrate how different lockdown plans might have impacted disease prevalence. Based on our observations, the transmission rate has the most significant impact on COVID-19 cases. This case study exemplifies the importance of carefully considering these issues before implementing lockdowns and social isolation throughout the country to combat future outbreaks.
Indentation creep behavior of Fe–8Ni–xZr oxide dispersion strengthened alloys
Authors: Tekin Mustafa , Muhaffel Faiz , Kotan Hasan , Baydoğan Murat
Published: July 2023

This study was conducted to understand the creep behavior of two oxide dispersion strengthened alloys containing Zr as the alloying addition by performing indentation creep tests at room temperature. The oxide dispersion strengthened alloys were Fe–8Ni–xZr (x = 1 and 4 at.%, i.e., Zr-1 and Zr-4 alloys, respectively), which had been previously fabricated by mechanical alloying; followed by consolidation via equal channel angular extrusion at 1000 °C. The indentation tests were conducted under a maximum load of 100 mN with the loading rates at 300 and 400 mN min−1. The hardness was calculated by the Oliver–Pharr method, and the creep properties, such as the creep displacement, creep strain rate, creep stress, and stress exponent n, were determined. The results showed that the Zr-4 alloy was harder than the Zr-1 alloy. However, the creep resistance of the Zr-1 alloy was better than that of the Zr-4 alloy. It was further demonstrated that both the hardness and creep resistance depended on the loading rate. Moreover, a possible creep mechanism was proposed. Although the tests were performed at room temperature, they can provide insight into the effect of an oxide dispersion strengthened alloys microstructure on creep at higher temperatures.
LAS PROTESTAS JUVENILES EN LA LIMA DEL BICENTENARIO
Authors: Orietta Marquina Veja
Published: July 2023

En noviembre del 2020, en plena pandemia del COVID19, bajo lemas como ¡Se metieron con la generación equivocada! ¡Merino no me representa! ¡Merino no es mi presidente! los jóvenes limeños y de muchas ciudades del interior del Perú salieron a las calles a expresar su protesta y a defender una institucionalidad que hasta el momento no habían sentido suya. Analizo los dispositivos estéticos intervinientes en los procesos de subjetivación política que confguran el protagonismo juvenil en las acciones colectivas en el espacio público limeño de hoy. Ellos reﬂejan su dimensión expresiva como espacio de acción convergente de las fuerzas de visibilización y enunciación que confguran su condición juvenil. Las prácticas desplegadas por los jóvenes con los dispositivos estéticos observadosdurante las protestas han sido relegadas a lo incidental o marginal, como si estas ocurrieran junto a lo político, de modo accesorio, y no como si fueran parte de undiscurso político mismo. Busco identifcar las narrativas políticas sobre la llamadaGeneración del Bicentenario que cuentan los dispositivos estéticos utilizados porlos jóvenes limeños en las protestas de noviembre 2020.
Understanding the Revival of Tehrik-i-Taliban Pakistan since FATA’s Merger
Authors: Younus Aisha , Hussain Tasawar , Khan Shehryar
Published: July 2023

How can we understand the resurgence of Tehrik-i-Taliban Pakistan (TTP) in the former Federally Administered Tribal Areas (FATA)? Framing FATA as a Zomian space, we argue that its “friction of terrain” provided nonstate spaces for the emergence as well as the resurgence of TTP. The literature describes TTP as an outcome of global political and security compulsions, Islamabad’s regional policies, and ideological associations with the Afghan Taliban. The present study adds terrain to the discussion. Islamabad, after militarily destroying TTP, announced the FATA merger in 2018. However, TTP’s revival not only problematizes the state’s role but also affirms the continuation of Zomian character: the nonstate spaces that facilitated TTP’s ability to regroup and thrive again. James C. Scott’s Zomia framework is tested here against empirical realities with the help of both primary and secondary data sources.
Effect of thermal treatment on the chemical composition and mechanical properties of birch and aspen
Authors: Kocaefe Duygu , Poncsak Sandor , Boluk Yaman
Published: July 2023

The high temperature treatment of wood is one of the alternatives to chemical treatment. During this process, the wood is heated to higher temperatures than those of conventional drying. The wood structure changes due to decomposition of hemicelluloses, ramification of lignin, and crystallization of cellulose. The wood becomes less hygroscopic. These changes improve the dimensional stability of wood, increase its resistance to micro-organisms, darken its color, and modify its hardness. However, wood also might loose some of its elasticity. Consequently, the heat treatment conditions have to be optimized. Therefore, it is important to understand the transformation of the chemical structure of wood caused by the treatment. In this study, the modification of the surface composition of the wood was followed with Fourier transform infrared spectroscopy (FTIR) and inverse gas chromatography (IGC) under different experimental conditions. The effect of maximum treatment temperatures on the chemical composition of Canadian birch and aspen as well as the correlations between their chemical transformation and different mechanical properties are presented. FTIR analysis results showed that the heat treatment affected the chemical composition of birch more compared to that of aspen. The results of IGC tests illustrated that the surfaces of the aspen and birch became more basic with heat treatment. The mechanical properties were affected by degradation of hemicellulose, ramification of lignin and cellulose crystallization.
Configuración estética en Gabriel García Márquez. Arte afirmativo: prejuicio y aplicación
Authors: Laverde Ospina Alfredo
Published: July 2023

Este artículo pretende mostrar cómo en algunas obras de Gabriel García Márquez se impone el carácter afirmativo del arte (en el sentido de H. Marcuse). Por una parte, por estar inscrito en una sociedad capitalista cuyos ideales burgueses tardíos ubican las elaboraciones críticas en el ámbito incontaminado de lo cultural, y, por otro, por la no resolución de continuidad fomentada por la tan socorrida autonomía del arte. Si bien no se niega la dimensión crítica presente en las obras del autor, la presentación de “valores auténticos” en el ámbito de lo estético “ideal” parece denunciar la imposibilidad de incidir en la transformación de la praxis social.
Editorial: El Caribe e Hispanoamérica: la literatura en los bordes
Authors: Caballero De la Hoz Amilkar
Published: July 2023

El número 32 de Cuadernos de Literatura del Caribe e Hispanoamérica incluye trabajos que analizan manifestaciones estéticas que se ubican en la liminalidad.Se trata de textos que se sitúan en un in-between espacial, temporal y temático, y que antes que afirmar e instituir verdades o nociones, expresan la vaguedad  y la indeterminación.
Hombres que la chupan: El vampiro de la colonia Roma (1979), masculinidades y vampirismo en América Latina
Authors: Quintero Santiago
Published: July 2023

El siguiente artículo presenta una mirada histórica y analítica de las narrativas sobre vampiros y su conexión con el problema de la masculinidad en América Latina. Partiendo de una lectura analógica de la novela El vampiro de la colonia Roma (1979), de Luis Zapata, exploro de qué manera las diferentes narrativas de vampiros en América Latina hacen visibles los saberes, los discursos y los dominios –biotecnológicos y culturales– acerca de los que se ha producido y continúa produciéndose la identidad masculina en el continente.
Propuesta de tradición discursiva de la narrativa de homosexualidad en México
Authors: Torres Valencia Ignacio
Published: July 2023

Con base en las tradiciones discursivas de Johannes Kabatek y mediante la lectura analítica de las novelas 41 o el muchacho que soñaba en fantasmas (1963), de Paolo Po; El diario de José Toledo (1964), de Miguel Barbachano Ponce; Los inestables (1968), de Alberto X. Teruel y Después de todo (1969), de José Ceballos Maldonado, se propone la existencia de una tradición discursiva de novelas de homosexualidad en México, partiendo de una decena de aspectos que se repiten en la mayoría de los textos mencionados, lo que pone de manifiesto una relación entre la conservación y la renovación de la manera de narrar estas historias.
Escritura geosimbólica en Vereda del norte (1937), de José U. Escobar, novela queer de la frontera norte mexicana
Authors: Urani Montiel Carlos
Published: July 2023

La novela Vereda del norte, del polifacético escritor mexicano José U. Escobar (1889-1958), se mantuvo inédita por más de medio siglo. Compuesta hacia 1937 en Ciudad Juárez y publicada en 2005, es una pieza clave de las letras norfronterizas mexicanas, particularmente, y de la literatura homoerótica latinoamericana, en general. El presente artículo analiza el texto mencionado a partir de cuatro ejes de estudio: los lazos afectivos de la pareja protagónica, los geosímbolos homoeróticos, el posicionamiento político desde la ficción histórica y la tradición de la escritura queer en la zona fronteriza de Ciudad Juárez-El Paso.
Editorial
Authors: Caballero De la Hoz Amilkar , Bolaño Sandoval Adalberto
Published: July 2023

Editorial
La visión del inocente
Authors: Chico Quintana Rafael Arturo
Published: July 2023

«Inmolar un animal / Y en silencio, en un terrible rezo de tu carne / devorar lentamente su ímpetu, su ansia / Saberte impuro entonces y gemir / en un terrible rezo de tu alma / Ahora, una vez más / nunca podrás perdonarte», sentencia el sujeto lírico del poema Ritual (Bustos, 2016, p. 196), y los versos bien parecieran un anuncio de Muerte de Dios y poesía moderna en Colombia, y es que esta última obra de Rómulo Bustos hace eco de estos versos al reflexionar sobre las consecuencias de una herida, ahora connatural a la cultura occidental y sobre todo moderna. La muerte de Dios, que fuera profesada como una nueva victoriosa o reclamo por la libertad de ser humano por-y-para-sí-mismo, en realidad tomó la forma de una problemática digerida con dificultad, una veces con renuencia, otras con negación, otras con aceptación acompañada de fervor disoluto, otras con ateísmo cientificista, pero secretamente místico, levantando cultos de capilla a ciertos predicadores de alguna versión del evangelio de la Razón. Es así que, con una  mirada menos pasiva que la de un testigo, pero más inocente que la de un hurgador de verdades cuya voz ha sido empeñada a las obligaciones de grupos o instituciones, Bustos hace arqueología del problema en su primer capítulo, titulado muy ilustrativamente Los avatares del Dios de la modernidad. Allí, la historia de esta problemática de larga data es reescrita entre los intersticios del discurso oficializado, y no hablo de deconstrucción, sino de una racionalidad sincera que rastrea orígenes, pero también indaga en sus proyecciones: desde Jean Paul Richter a Nietzsche, desde el romanticismo hasta su legado para la modernidad, desde el libertino hasta el poeta maldito… desde el Discurso del Cristo muerto, el cual, desde lo alto del edificio del mundo, proclama que Dios no existe (J. P. Richter) hasta sus resonancias futuras, y es gracias a ello que la voz decidida de un Bustos, sin el menor asomo de temor frente los azarosos rostros de una defunción de lutos prolongados, nos lleva comprender una de las causas profundas del constante desencanto, pesimismo, melancolía y dilatada y reincidente rebeldía de mucha de la poesía moderna hasta nuestros días, habitada de sujetos líricos cuya voz sólo sabe enunciar con disonancia, incertidumbre y enigmatismo. Con esa forma de decir que el trabajo clásico de Hugo Friedrich supo describir, pero que ahora Rómulo Bustos nos explica, estudiando el limo desde el que crece y se alimenta.
Nueva Poesía de Colombia (1970-1980)
Authors: García Quintero Felipe
Published: July 2023

Por tratarse de unas poéticas la mayoría en configuración, de obras en marcha, herederas de una tradición insular, la ruta crítica seguida en este artículo orienta al lector sobre el devenir de la poesía colombiana en sus actuales tendencias y corrientes. La muestra poética adjunta de 20 autores es su prueba. Una muestra de la más reciente generación de poetas colombianos del siglo XXI, conformada por autores nacidos entre 1970 y 1980. La reflexión final y la compilación de obras llaman la atención del lugar que ocupan las voces femeninas en esta generación, caracterizada por la pluralidad temática y diversidad de estilos.
Bajo el brillo de la luna: de edvard munch a la poesía de Nelson Romero Guzmán
Authors: Gaitán Bayona Jorge Ladino
Published: July 2023

El objetivo de este artículo es analizar la intertextualidad entre poesía y pintura en el libro Bajo el brillo de la luna, a partir del concepto de ecfrasis. Se toman planteamientos teóricos y metodológicos de William Mitchell, Michael Rifaterre, Pedro Agudelo y Luz Pimentel. La hipótesis es que el poeta recrea pinturas de Edvard Munch para explorar el proceso de la creación artística.  Se concluye que el libro aporta a la lírica colombiana basada en la pintura. Sus poemas reinventan a Munch y exaltan el arte como morada digna cuando el autor sufre locura, desencanto y miedo a la muerte.
Piedad Bonnett en la poesía colombiana
Authors: Rodriguez Peña Adriana
Published: July 2023

Este artículo propone una visión comparativa de la poesía para ubicar la propuesta estética de Bonnett en la poesía colombiana, así como las características  y representantes de su generación.
Editorial
Authors: Caballero De la Hoz Amilkar
Published: July 2023

Editorial
La obra literaria de Fernández de Lizardi ante sus críticos contemporáneos: entre amigos y enemigos
Authors: Ortiz Sánchez Ma. de Lourdes
Published: July 2023

En la Nueva España privó la discriminación social y racial. A finales del XVIII surgió Lizardi, quien enfrentó críticas entre personales y académicas por sus ideas. El objetivo es estudiar la recepción de sus obras entre sus contemporáneos, la pregunta eje es ¿actuaban de manera justificada, sus críticas son académicas o bien las impulsó la ira y el deseo de ridiculizar? El método de análisis es la lectura simultánea, de acuerdo al orden de la escritura; se partirá del contexto colonial de pugnas y después se confrontará al Pensador con sus críticos; se concluye que en general acometieron sin considerar las aportaciones.
De la imagen a la imaginación acústica: Pausada percusión y otras memorias de Julio César Goyes Narváez
Authors: Rojas Ramírez Carlos
Published: July 2023

En Youth, de Paolo Sorrentino, Michael Caine interpreta a un compositor y director de orquesta que es capaz de hacer hablar a la naturaleza con sus propias manos. Su mirada, en la mayoría de las escenas, refleja un tempo abisal, mientras persiste un cierto equilibrio entre sonido y silencio que anega cada de uno de los cuadros de la película, como si fuese una mancha tipográfica que derramase palabras entre blancos y negros.
Panorama continental de la intervención cultural y los efectos políticos de la práctica crítica. Caso especial de Hernando Téllez y José Lins do Rego
Authors: Laverde Ospina Alfredo
Published: July 2023

La crítica literaria cultivada en América Latina durante las décadas del 40 y 50 del siglo XX tuvo como una de sus funciones principales la intervención en la definición de políticas culturales. De ahí que este artículo de reflexión se inicie con la presentación breve de un panorama de la crítica literaria latinoamericana y haga especial énfasis en dos escritores y críticos literarios, el brasileño José Lins do Rego y el colombiano Hernando Téllez S. En términos generales, mediante el comentario crítico de ensayos de los autores, el objetivo primordial de este artículo es resaltar la existencia de una peculiar autonomía de la crítica literaria continental.
Locas de felicidad, cronicas travestis y otros relatos, a la luz de Pedro Lemebel
Authors: Rivera Pinilla Diana
Published: July 2023

En torno a la investigación con enfoque en género e identidad sobre el libro Locas de felicidad. Crónicas travestis y otros relatos (2009) del escritor colombiano John Better Armella, se ha propuesto una intertextualidad directa entre este texto y el proyecto literario del escritor chileno Pedro Lemebel. Dicha intertextualidad respalda, como se observará en el presente artículo, la construcción de una comunidad literaria que alimenta una tradición homoerótica latinoamericana, al descubrir una simbiosis artística, estética y axiológica que se presenta entre la obra de Better y obras como Loco afán: crónicas de sidario (1996) o Tengo miedo torero (2001).
Reflections on 50 Years of Experience in Educational Technology
Authors: SPECTOR Jonathan Michael
Published: July 2023

I came to the world of educational technology in the 1980s from a position as a computer science professor at a regional state university in Alabama where I had been practicing as an educational technologist without awareness that I was doing so. In the intervening years, I have come to realize the complexities and challenge of educational technology and instructional design, thanks to mentors such as Robert Gagné, David Merrill, Robert Tennyson, and many others too numerous to name. Those learning experiences were largely unplanned and happened more or less by coincidence as I left teaching computer science to become the senior scientist for instructional systems at the Air Force Human Recourses Laboratory (a.k.a. Armstrong Laboratory) in San Antonio, Texas. I have also had the pleasure of being a tenured professor at the University of Bergen, Syracuse University, Florida State University, the University of Georgia, and the University of North Texas, where I have tried to apply the lessons learned along the way. Regretfully, what I see now happening in the broad domain of educational technology is a return to the beliefs I had when I entered this field so many years ago. Those unaware of the complexities and challenges and untrained in educational technology and learning science but adept with one or more technologies believe they can apply their technology knowledge to solve any educational problem that arises. If these remarks have any benefit, it is to help those technophiles understand that the emphasis and focus in educational technology and learning science should be placed initially and primarily on the learner and not on a particular technology nor the content to be learned. Technologies come and go, and those with keep knowledge in a content domain have already indicated what can be learned in that domain. As a result of my focus on the individual learner in this paper, this will not be a typical academic research paper. Rather it will be a plea to consider what really matters – not one’s own prominence or advancement but helping others develop their own thoughts and understanding. Onward through the fog (a slogan borrowed from Oat Willie’s in Austin, Texas).
Suspicion of a teenager with Duchenne disease in the pediatrics department of Sainte Thérèse De Hinche Hospital
Authors: Berley Alphonse , Michelande Elien , Edisond Florial , Andorie Samanda Labonte , Chrismy Augustin , Phélès Dagerus
Published: July 2023

Duchenne disease is caused by a deletion of the gene coding for dystrophin. The absence of this protein is responsible for the myonecrosis observed during the evolution of the pathology. It is X-linked recessive which explains its occurrence, especially in boys. The manifestation of the disease begins in 3 years - 5 years, and the life expectancy is 20 years - 30 years. In the classic clinic, we have gait disorders, kyphoscoliosis, pseudo hypertrophy of the calves, and a positive Gower’s sign. Cardiorespiratory impairment is often incriminated as the cause of death in these patients. Management is based on physiotherapy and corticosteroid therapy. In the event of a cardio-respiratory manifestation, the use of positive pressure ventilation, and anti-hypertensives such as ACE inhibitors and beta-blockers may be necessary. We will discuss the 10 years old patients who present with the classic symptoms of Duchenne disease and how we managed it in a low-income country.
Nanopore Influenza A Virus WGS using one step RT/PCR with RBK barcoding v1
Authors: Williams Tom
Published: July 2023

A protocol to generate Influenza A Virus sequences from RNA extracts in under 24 hours with less than two hours of hands on time. Based on: King, J., Harder, T., Beer, M., & Pohlmann, A. (2020). Rapid multiplex MinION nanopore sequencing workflow for Influenza A viruses.BMC Infectious Diseases,20(1). https://doi.org/10.1186/s12879-020-05367-y Zhou, B., Donnelly, M. E., Scholes, D. T., st. George, K., Hatta, M., Kawaoka, Y., & Wentworth, D. E. (2009). Single-Reaction Genomic Amplification Accelerates Sequencing and Vaccine Production for Classical and Swine Origin Human Influenza A Viruses.Journal of Virology,83(19), 10309–10313. https://doi.org/10.1128/jvi.01109-09 Tested with up to 20 RNA extracts from samples per run, alongside: Negative control of nuclease-free water Positive control of H1 Positive control of H3 Positive control of lambda phage, introduced at barcoding stage (i.e. not amplified), as an assessment of barcode crosstalk
A Study on the Impact of Artificial Intelligence Anxiety on the Innovation-Oriented Behaviours of Employees
Authors: ÖZTIRAK Mesut
Published: July 2023

The main purpose of this study is to investigate the effect of artificial intelligence anxiety on innovation-oriented behaviours of employees. In addition, it was examined whether demographic characteristics differ on artificial intelligence anxiety and innovation-oriented behaviours. The universe of the research consists of private sector employees working in Istanbul in 2022. Using the convenience sampling method, 412 private sector employees were reached between 26.06.2022 and 26.08.2022. Data analysis was done with SPSS 24.0. T test and ANOVA test were used in the analysis of demographic variables. The relationship between the scale items was analysed with the Pearson correlation test. The effect between the scale items was analysed with the regression test. According to the results of the analysis, artificial intelligence anxiety has a positive and significant effect on the innovation-oriented behaviours of the employees. (p
Interleukin 6 Inhibition in Rheumatoid Arthritis: Highlight on Olokizumab
Authors: Feist Eugen ,   Helios Department for Rheumatology and Clinical Immunology, Vogelsang-Gommern, Germany, Nasonov Evgeny ,   V. A. Nasonova Research Institute of Rheumatology, Moscow, Russia,   Department of Rheumatology, Institute for Professional Education, I. M. Sechenov First Moscow State Medical University, Ministry of Health of Russia, Moscow, Russia
Published: July 2023

Rheumatoid arthritis (RA) is a chronic immunoinflammatory rheumatic disease, which manifests as progressive destruction of joints, systemic inflammation of visceral organs and a wide range of comorbidities associated with chronic inflammation. Among the cytokines involved in the pathogenesis of RA and certain other immunoinflammatory rheumatic diseases, the role of interleukin (IL) 6 is of special interest. The introduction of the monoclonal antibodies tocilizumab and later sarilumab, both of which block the IL-6 receptor, into clinical practice was an important achievement in the treatment of immunoinflammatory rheumatic diseases at the beginning of the 21st century. The humanized monoclonal antibody against IL-6, olokizumab, provides a new mode of action by direct inhibition of IL-6. This article reviews new data on the efficacy and safety of olokizumab in RA and the prospects of its use in rheumatology.
Strategies to Improve Adherence in Patients with Rheumatoid Arthritis
Authors: Sun Margaret Man-Ger ,   Schulich School of Medicine & Dentistry, London, ON, Canada,   Western University, London, ON, Canada,   Department of Medicine, Division of Rheumatology, St Josephs Health Care, London, ON, Canada, Pope Janet E ,   Schulich School of Medicine & Dentistry, London, ON, Canada,   Western University, London, ON, Canada,   Department of Medicine, Division of Rheumatology, St Josephs Health Care, London, ON, Canada
Published: July 2023

Rheumatoid arthritis is a chronic inflammatory condition that can significantly affect patients and society at large due to decreased quality of life, direct and indirect costs and subsequent socioeconomic burden. Medication adherence plays a big role in optimal disease management, as higher adherence is associated with lower disease activity. We examine the differences between intentional and non-intentional non-adherence and suggest potential solutions on how to integrate this understanding into clinical practice to improve medication adherence.
The Adaption and Validation Processes in Remission Criteria for Rheumatoid Arthritis
Authors: Studenic Paul ,   Division of Rheumatology, Department of Medicine (Solna), Karolinska Institutet, Stockholm, Sweden,   Division of Rheumatology, Department of Internal Medicine 3, Medical University of Vienna, Vienna, Austria
Published: July 2023

The standardization of disease activity assessment in patients with rheumatoid arthritis has improved the comparability of clinical trials. In 2011, the American College of Rheumatology and the European Alliance of Associations for Rheumatology provisionally endorsed the remission criteria for RA; since then, the criteria have been the subject of debate in terms of whether they are too lenient or too stringent. The Patient Global Assessment (PtGA) was one focus of the debate, with a higher PtGA threshold for the Boolean remission criteria being proposed. After validation in 2022, the revised Boolean criteria included a 2 cm PtGA cut-off, with the index-based definitions receiving full endorsement from the American College of Rheumatology and the European Alliance of Associations for Rheumatology.
A review on ebola virus
Authors: Sadgir Pallavi B , Pawar Pradnya R , Jondhale Prachi S , Sharmale Manisha N , Ambre Priya , Phalke Pallavi L
Published: July 2023

The Ebola virus of the Filoviridae family is the cause of Ebola virus disease (EVD), a deadly viral hemorrhagic sickness. Due to the prevalence of immigrants, the disease has become a global public health threat. The victims initially exhibit vague influenza-like symptoms before succumbing to shock and multiorgan failure. There is no established procedure for treating EVD; instead, only supportive and symptomatic therapy is used. The Ebola virus, including its clinical and oral symptoms, diagnostic tools, differential diagnoses, preventive measures, and management protocol, are thoroughly discussed in this review paper. Since then, the Ebola virus has occasionally started to infect humans, causing multiple epidemics. The expansion of the Ebola virus has resulted in the deadliest diseases for both animals and humans because of the growth of urbanization, invasion of forested areas, and intimate contact with wildlife creatures. The Ebola virus disease (EVD) has so far claimed the lives of numerous people, with an increased number of cases being seen throughout the African continent. Thus, a study was conducted to evaluate the efficacy and safety of medications approved for the treatment of EVD, trends in EVD outbreaks, morbidity and mortality among EVD patients, and other factors.
Tapentadol, an opioid as a strategy for the treatment of chronic pain? A narrative review
Authors: Silva Alessandra Oliveira , Marrafon Danielle Aparecida de Oliveira , Amorim Ana Flávia , Barros Carlos Marcelo de , Rascado Ricardo Radighieri , Ceron Carla Speroni , Reis Tiago Marques dos , Podestá Márcia Helena MIranda Cardoso , Almeida Daniel Augusto de Faria , Torres Larissa Helena , Pereira Marília Gabriella Alves Goulart
Published: July 2023

Chronic pain affects approximately 30 % of the world population. Tapentadol can be an analgesic option for patients who do not respond adequately to commonly used opioids. This study reviewed the general aspects of Tapentadol pain treatment and its possible association with increased oxidative stress, as well as the benefits of its association with antioxidant substances. The search was carried out in the Medline (Pubmed), Scopus, Web of Science, and Google academic databases, including studies since the registration of the drug in 2008. The selected articles were those related to the use of Tapentadol for chronic moderate to severe pain, but not cancer-related pain, in adults and the elderly. Tapentadol is a µ opioid receptors agonist and inhibits noradrenaline reuptake. Although Tapentadol causes fewer adverse effects than other conventional opioids, studies have shown the induction of oxidative stress by this drug, but without having elucidated the mechanisms.
How Do Migrants Fare in Latin America and the Caribbean?: Mapping Socio-Economic Integration
Authors:   Inter American Development Bank,   Organization for Economic Cooperation and Development,   United Nations Development Programme
Published: July 2023

Over the last decade, the migration landscape in Latin America and the Caribbean (LAC) has changed significantly. In this context, the socio-economic integration of immigrants is an increasingly high priority on the regional development and policy agenda. For this reason, the Inter-American Development Bank (IDB), the Organisation for Economic Cooperation and Development (OECD), and the United Nations Development Programme (UNDP) have collaborated on this joint exercise that builds on OECDs previous experience in measuring migrant inclusion as well as IDBs expertise in building data around the state of migration in Latin America and the Caribbean, and UNDPs presence on the ground and experience working with national and local governments in the region to advance their development priorities. This report provides a general overview of the state of socio-economic integration of migrants in 12 LAC countries by 2021. It presents a series of quantitative indicators related with, for instance, labor market informality, self-employment, youth employment, school attendance, reading literacy and living conditions. This exercise also relies on selected policy indicators that shed light on the regulatory framework within which migrants integration takes place. The objective is to provide decisionmakers and policymakers in host countries with useful indicators to better understand where the gaps are in terms of migrants integration and to help them identify the areas where they should focus their efforts and scarce resources.
Robotic Appendicectomy
Authors: Arang Hossein , El Boghdady Michael
Published: July 2023

Acute appendicitis is one of the most common abdominal emergencies. There has been an increasing news of robotic abdominal surgery. However, it remains underutilised in emergency settings. This study aimed to systematically review robotic appendicectomy (RA) feasibility. A 20-year systematic review was performed, along with quality assessment. The research protocol was registered with PROSPERO. The search yielded 1,242 citations, including 9 articles. The mean quality score was 10.72 ± 2.56. The endpoints across the studies were rate of conversion to open surgery, length of hospital stay, blood loss and operative time. RA is a safe, feasible technique that can be performed in elective and emergency settings with minimal blood loss. The operative time and hospital stay were within acceptable limits. Robotic surgery’s major drawback is its high cost and limited availability. Future studies evaluating RA with a focus on its application during emergencies and its cost-effectiveness are recommended.
Keywords: Robot Surgery; Robotic-Assisted Surgery; Robot Enhanced Surgery; Robotic Surgical Procedure; Appendectomy; Appendicectomy; Gastrointestinal Surgical Procedure.
A puericultura e os desafios decorrentes da pandemia de COVID-19
Authors: Smaniotto Madeira Maria Eduarda , Wisniewski Setter Nathalia , Laschewitz Wamser Johanna , Marcon Leticia , Maysa de Souza Daniela
Published: July 2023

Com este estudo buscou-se analisar pela perspectiva médica, a evasão nas consultas de puericultura durante a pandemia de COVID-19. Trata-se de uma pesquisa qualitativa e descritiva, que foi realizada com cinco médicos atuantes nas Estratégias de Saúde da Família localizadas na cidade de Blumenau, do Médio Vale do Itajaí, em Santa Catarina. A coleta de dados foi realizada nos meses de julho e agosto de 2022, por meio de entrevista (gravada e transcrita), utilizando um questionário semiestruturado, por meio do método bola de neve, sendo escolhida a análise temática para análise dos dados. A pesquisa foi aprovada sob nº CAAE 58302622.0.0000.5370. A pandemia de COVID-19 impactou negativamente no acompanhamento das consultas de puericultura e as principais repercussões relacionam-se à diminuição das consultas; atraso no acompanhamento do desenvolvimento neuropsicomotor; aumento dos casos respiratórios com o retorno das crianças às creches e baixos índices de cobertura vacinal. Além disso, aspectos nutricionais foram evidenciados, como desmame precoce e mudanças dos hábitos alimentares (com aumento ou perda ponderal). Até o momento, as implicações acerca da COVID-19 na puericultura se tornaram inestimáveis, desta forma, os dados do estudo trazem dados relevantes para o planejamento de estratégias de fortalecimento das ações de puericultura, de promoção da saúde e prevenção de doenças.
Improvement of technological processes of cultivation of vegetable crops in the Far East. Review
Authors: Sakara N. A. , Tarasova T. S. , Oznobikhin V. I.
Published: July 2023

The results of the selection work of the Primorsky vegetable experimental station, work in the field of seed production of vegetable crops are presented, the characteristics of the negative processes occurring in the arable soils of the region and their removal by the developed ridgeridge technology of cultivation by technical means with a wide base are given. A comparative characteristic of the former and the wide-row version in terms of energy consumption and fuel consumption is givenAuthors´ Contribution: All authors contributed to the planning and setting up the experiment, aswell as in the analysis of experimental data and writing of the article.Based on the results of the selection work of the Primorsky Vegetable Experimental Station for the period 1993-2023, the station bred and released 56 varieties and hybrids of 19 main vegetable crops. The experience of the Primorsky branch of the Federal scientific vegetable center allows organizing production at a modern level, taking into account the rich experience of other regions of Russia. In conditions of monsoon climate and soils of heavy granulometric composition, with a general lack of heat, their fertility is negatively affected by a large number of external factors. To grow vegetables, a set of measures is needed for serious drainage and irrigation reclamation (creation of polder systems with the laying of systematic plastic drainage) against the backdrop of ridge-ridge cultivation technology, fine irrigation system). The developed wide-cut technology and a set of machines make it possible to introduce a resource-saving technology that saves energy costs and fuel.
Using Photovoice to Illuminate Challenges Facing Children with Disabilities in Inclusive Education in Ghana
Authors: Asamoah Edward , Amaniampong Er-Menan , Manful Esmeranda , Gyasi-Boadu Nelson , Nana Mbrah Koomson-Yalley Elizabeth
Published: July 2023

Achieving inclusive and quality education for all children remains a significant problem amidst several policies in sub-Saharan Africa. This study explores challenges facing children with disabilities in mainstream inclusive education-based basic schools in a sub-Saharan Ghanaian context. A qualitative photovoice approach was employed to highlight the experiences of six (6) pupils with disabilities. Findings revealed three main themes: unfriendly environments, inaccessible physical structures, and lack of assistive equipment, reflecting significant challenges confronting children with disabilities in inclusive education settings. Social support from colleagues without disabilities, however, acted as a major coping resource for pupils with disabilities. The study has implications for policy an
Sıvı vermikompost ve mikrobiyal gübre uygulamalarının toprağın bazı kimyasal ve biyolojik özellikleri ile marul bitkisinin (Lactuca sativa L.) verimi üzerine etkileri
Authors: ERGÜN UZUNOĞULLARI Revna , OKUR Nur
Published: July 2023

Amaç: Bitki gelişimini uyaran rizobakterileri (PGPR) içeren bir mikrobiyal gübre ile sıvı vermikompost ve kimyasal gübre uygulamalarının marul bitkisinin kök gelişimi, verimi ve bazı toprak özellikleri üzerine etkilerinin incelenmesi amaçlanmıştır.
Materyal ve Yöntem: Deneme konuları: 1) NPK%100, 2) Sıvı Vermikompost, 3) Mikrobiyal Gübre+ Sıvı Vermikompost 4) Mikrobiyal Gübre, 5) NPK (%50) + Sıvı Vermikompost, 6) NPK (%50) + Mikrobiyal Gübre, 7) NPK (%50) + Mikrobiyal Gübre+ Sıvı Vermikompost. Bitkilerin 10 hafta sonra hasatları yapılarak pazarlanabilir baş ağırlıkları ve kök gelişimleri belirlenmiş ve toprak örneklerinde bazı mikrobiyolojik ve kimyasal analizler yapılmıştır.
Araştırma Bulguları: Uygulamaların toprağın kimyasal (pH, toplam tuz, organik madde, toplam N, alınabilir P ve K) ve mikrobiyolojik özellikleri (toplam genel bakteri sayısı ve toprak solunumu) ile marul bitkisinin kök gelişimi, verimi ve azot alımı üzerine etkisi istatistiki anlamda önemli bulunmuştur.
Sonuç: Mikrobiyel gübre ve sıvı vermikompost ile kombine edilen kimyasal gübrelerde %50 azalmanın verimde bir kayba neden olmadığı ve bu uygulamaların kimyasal gübre uygulamasına oranla verimi yaklaşık %26 oranında artırdığı saptanmıştır.
Strategies for integrating a web-based learning environment based on authentic learning in distance learning for elementary school students
Authors: Hidayati Abna , Eldarni  , Solfema  , Handrianto Ciptro , Sunarti Vevi
Published: July 2023

This research aimed to find a strategy to integrate web-based learning based on authentic learning by identifying the dominant factors regarding teachers' authentic activities. The research method was a teacher survey that used web-based learning. Questionnaires were given to 100 teachers regarding the use of authentic activities. The questionnaire was tested for validity and had a high load factor of more than 0.6. Authentic learning had an effect of 0.689. The data was processed by factorial analysis to obtain the dominant value. Furthermore, a strategy to integrate web-based learning was developed in learning. The results showed that the dominant authentic learning activities used were real-world activities, integrated assessment, various sources   and final product design. It can be concluded that using web-based learning applications based on authentic learning provides real-life experiences for students and supports learning to be more meaningful.
Effect of Cross‐Linking Agent on Curcumin Release from Chitosan‐Based Macroparticles
Authors: Pistone Alessandro , de Gaetano Annamaria , Piperopoulos Elpida , Abate Chiara
Published: July 2023

This work deals with the synthesis of bare and curcumin (CUR)-loaded chitosan (CS)-based macroparticles by ionic gelation using sodium hydroxide (NaOH) or sodium tripolyphosphate (TPP) as cross-linking agents. The resulting spherical-shaped macroparticles were studied using various characterization techniques, Scanning Electron Microscopy (SEM), Fourier Transformed Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), and Differential Scanning Calorimetry (DSC). The release of CUR from the CS-based particles with respect to time was analyzed, and the encapsulation efficiency and degree of swelling were studied. All formulations showed excellent CUR trapping efficiency, exceeding 90%. In particular, the TPP-crosslinked macrobeads released 34 wt% of the charged CUR within minutes, while the remaining 66 wt% was released slowly due to the biodegradation of CS. The results indicate that the correct choice of gelling agent and its concentration leads to spherical particles capable of encapsulating CUR and releasing it in a wide range of kinetics depending on the specific application.