bluuebunny/crossref_metadata_2025 huggingface dataset:
Apolipoprotein A-I priming via SR-BI and ABCA1 receptor binding upregulates mitochondrial metabolism to promote insulin secretion in INS-1E cells
Authors: Lyons Claire L. , Cowan Elaine , Nilsson Oktawia , Mohar Manca , Peña-Martínez Pablo , Eliasson Lena , Lagerstedt Jens O.
Published: November 2024

Apolipoprotein A-I (ApoA-I), the primary component of high-density lipoprotein (HDL) cholesterol primes β-cells to increase insulin secretion, however, the mechanisms involved are not fully defined. Here, we aimed to confirm ApoA-I receptors in β-cells and delineate ApoA-I-receptor pathways in β-cell insulin output. An LRC-TriCEPS experiment was performed using the INS-1E rat β-cell model and ApoA-I for unbiased identification of ApoA-I receptors. Identified targets, alongside ATP binding cassette transporter A1 (ABCA1) (included control) were silenced in the same cells, and insulin secretion (ELISA) and mitochondrial metabolism (seahorse) were assessed with/without ApoA-I priming. Human β-cell expression data was used to investigate ApoA-I receptor pathways in type 2 diabetes (T2D). Scavenger receptor B1 (SR-BI) and regulator of microtubule dynamics 1 were identified as ApoA-I targets. SR-BI or ABCA1 silencing abolished ApoA-I induced increases in insulin secretion. ApoA-I priming increased mitochondrial OXPHOS, however this was greatly attenuated with SR-BI or ABCA1 silencing. Supporting this, human β-cell expression data investigations found SR-BI and ABCA1 to be correlated with genes associated with mitochondrial pathways. In all, SR-BI and ABCA1 correlated with 73 and 3 genes differentially expressed in T2D, respectively. We confirm that SR-BI and ABCA1 are the primary β-cell ApoA-I receptors and demonstrate that ApoA-I priming enhances β-cell insulin secretion via the upregulation of mitochondrial metabolism through ApoA-I-SR-BI and ApoA-I-ABCA1 pathways. We propose that SR-BI relies on mitochondrial and exocytotic pathways, while ABCA1 depends solely on mitochondrial pathways. Our findings uncover new targets in ApoA-I β-cell mechanism for T2D therapies.
Decoding lymphangiogenesis in oral squamous cell carcinoma: Emphasis on clinical and histopathological determinants of regional metastasis
Authors: Tandon Ankita , Sandhya Kumari , Singh Narendra Nath , Gulati Nikita , Kumar Amit , Sethi Kanika
Published: November 2024

Purpose

The growth and metastasis of solid epithelial tumors is lymphangiogenesis dependent. The most important lymphangiogenic inducers facilitating this progression is Vascular endothelial Growth Factor C (VEGF-C). The recent D2-40 (Podoplanin) antibody is specific for lymphatic epithelium and allows its objective assessment. Also, lymphovascular invasion (LVI) is a risk factor for lymph node metastases (LNM) and indicates a significant influx of tumor cells into the lymphatics, causing regional metastasis. Thus, the following study was conducted to assess and correlate VEGF-C and D2-40 immunoexpressions with clinical and histopathologic lymph node status in Oral Squamous Cell Carcinoma (OSCC) cases and also to estimate the impact of intratumoral (ILVD) and peritumoral lymphatic vessel density (PLVD) in such cases.

Methodology

A total of 128 OSCC cases, divided as Group I: Cases with clinically and histopathologically negative lymph nodes (n = 64) and Group II: Cases with clinically negative but histopathologically positive lymph nodes (n = 64) were immunoscored for VEGF-C (Anti VEGF-C antibody) (PA5-29772, Invitrogen) and D2-40 (Anti D2-40 antibody) (IR072/8072, Dako) using standard protocols. The data was statistically evaluated using STATA 18.0 with p≤0.05 considered statistically significant throughout the study.

Result

21.88% Group I cases and 40.62% Group II cases showed highest immuno-positivity for VEGF-C (p = 0.00). The mean D2-40 score for Intratumoral Lymphatic Vessel Density (ILVD) and Peritumoral Lymphatic Vessel Density (PLVD) was higher for group II cases (i.e., 41.5±13.73 and 35.95±8.27 respectively at 95% CI, p = 0.00) suggesting a direct correlation between Lymphatic Vessel Density (LVD) and LNM.

Conclusion

Lymphangiogenesis is a true determinant of the biologic potential of OSCC and obtaining an objective data in terms of LVD through D2-40 could be impactful in OSSC diagnosis and guiding treatment decisions by clinicians.
LincROR promotes tumor growth of colorectal cancer through the miR-145/WNT2B/WNT10A/Wnt/β-catenin regulatory axis
Authors: Deng Li-Qiang , Li Shi-Ying , Xie Tian , Zeng Wei-Qiang , Wang Yu-Yan , Shi Chuan-Jian , Jin-Fang Zhang
Published: November 2024

Colorectal cancer (CRC) is a prevalent form of malignant tumor, and the current clinical treatments are far from satisfactory. Identifying new therapeutic targets is therefore essential for clinical practices. The long intergenic non-protein coding RNA lincROR has been shown to play a significant role in the tumorigenesis of various cancers. However, the molecular mechanism underlying lincROR-mediated CRC tumorigenesis remains unclear. In the present study, we found that knockdown of lincROR significantly inhibited cell viability in vitro, while its overexpression promoted tumor growth in vivo. Mechanistically, lincROR acted as a miRNA sponge for miR-145, thereby elevating the expression of the target genes WNT2B and WNT10A. The overexpression of WNT2B and WNT10A definitely activated the Wnt/β-catenin pathway, thus led to promoting tumorigenesis in CRC. In summary, our findings identified lincROR as a novel activator of the Wnt/β-catenin pathway by serving as a miRNA sponge for miR-145 and facilitating tumorigenesis, which suggests that lincROR may be a potential therapeutic target for CRC patients.
Effect of poor glycemic control on the prevalence and determinants of anemia and chronic kidney disease among type 2 diabetes mellitus patients in Jordan: An observational cross-sectional study
Authors: Al-Dwairi Ahmed , Al-Shboul Othman , Al-U’datt Doa’a G. F. , Saadeh Rami , AlQudah Mohammad , Khassawneh Adi , Alfaqih Mahmoud , Albtoush Alhakam , Hweidi Aysam , Alnemer Abdulaziz
Published: November 2024

Background and objectives

Anemia and chronic kidney disease (CKD) are common findings in diabetic patients. Lack of glycemic control is associated with increased risk of diabetic complications. This study aimed to determine the effect of poor glycemic control on the prevalence and determinants of anemia and CKD among type 2 diabetes mellitus (T2DM) patients in Jordan.

Methods

A cross-sectional study design was used in this research. T2DM patients with controlled diabetes (HbA1c ≤7.0%, n = 120) and age-, gender- and body mass index–matched uncontrolled diabetic patients (HbA1c >7.0%, n = 120) were recruited. Blood sample for HbA1c and serum insulin measurement were obtained. Complete blood count and kidney function test results were obtained from the patient’s medical records. Anemia was determined according to World Health Organization criteria. A binomial logistic regression was performed to ascertain the effects of age, gender, CKD and glycemic control on the likelihood that participants have anemia.

Results

The prevalence of anemia was significantly higher in the uncontrolled T2DM compared to controlled T2DM patients (40% vs 27.5%, OR: 2.14, 95% CI: 1.23, 3.71, P = 0.006). Female patients with uncontrolled T2DM had significantly greater prevalence of anemia compared to male patients with uncontrolled T2DM. The binomial logistic regression analysis showed that age, female gender, and CKD were positively associated with anemia in the multivariate model, while in the univariate model, lack of glycemic control increases the odds of anemia by 1.74 (95% CI: 1.01, 2.99, P = 0.046).

Conclusion

Anemia is commonly present among T2DM patients in Jordan and is associated with poor glycemic control especially in females. These results emphasize the necessity of including anemia screening in standard diabetes care to enable early detection and treatment of anemia and to enhance the overall care of diabetic patients.
Manufacturing exosomes for wound healing: Comparative analysis of culture media
Authors: Dao Huy Hoang , Nguyen Thu-Huyen , Hoang Diem Huong , Vu Bach Duong , Tran Minh-Anh , Le Mai Thi , Hoang Nhung Thi My , Bui Anh Viet , Than Uyen Thi Trang , Nguyen Xuan-Hung
Published: November 2024

Mesenchymal stem cell (MSC)-derived exosomes (EXs) have emerged as promising therapeutic agents for wound healing. However, the optimal conditions for manufacturing MSC-derived EXs that maximize their wound-healing potential have yet to be established. Hence, we compared the efficacy of five different MSC culture media, including three different serum-free, a platelet-supplemented, and a fetal bovine serum-supplemented media, in exosome manufacturing for wound healing applications. Although umbilical cord-derived MSCs (UCMSCs) cultured in these media exhibited similar proliferation, morphology, MSC surface marker expression, and stemness, EXs derived from UCMSCs cultured in different culture media displayed varying levels of growth factors and cytokines. Notably, EXs derived from platelet-supplemented media (DM-PLT_EXs) exhibited significantly higher concentrations of keratinocyte growth factor (KGF), vascular endothelial growth factor (VEGF-A), platelet-derived growth factor (PDGF-BB), interleukin 6 (IL-6), interleukin 7 (IL-7), and interleukin 8 (IL-8) than EXs from other media. These differences correlated with the superior capability of DM-PLT_EXs to promote human skin fibroblast proliferation and stimulate angiogenesis of human umbilical vein endothelial cells, making them a more suitable choice for wound healing applications. Our findings emphasize the significance of the culture medium selection in tailoring the therapeutic potential of UCMSC-derived EXs for wound healing.
The feasibility of combining greening schoolyards and nutrition education in primary schools: A qualitative study
Authors: van Wijk Geertje , Antwerpen Lisa van , Hoefnagels Femke A. , Staps Sjef J. J. M. , Battjes-Fries Marieke C. E.
Published: November 2024

Objective

An increasing number of children in the Netherlands is overweight or obese, which is largely attributable to an unhealthy lifestyle and unhealthy living environment. Nutrition education and greening the schoolyard, for example with a vegetable garden, have independently been studied and are shown to be effective in teaching children a healthy lifestyle and providing a healthy living environment. However, the feasibility of combining nutrition education and greening the schoolyard has not been studied yet. Therefore, this study aimed to provide insight into primary schools’ vision on making this combination, and the feasibility of doing so.

Methods

In this study a qualitative research design was used. The theoretical frameworks of Proctor et al. and Sekhon et al. were used to develop the interview guide. Twelve semi-structured interviews were conducted with members of school teams and school directors. The interviews were transcribed verbatim and analyzed using the thematical analysis of Braun and Clarke.

Results

According to the interviewees, three facets are essential to make the combination of greening schoolyards and nutrition education feasible and successful. Firstly, the interviewees mentioned that the school team and school directors of schools must be able to see the connection between greening the schoolyard and nutrition education. Additionally, support is needed among the parents, school team, pupils and local community. Finally, the interviewees stated that schools must be able to integrate greening the schoolyard and nutrition education into their existing curriculum.

Conclusions

The results of this study showed that combining greening schoolyards and nutrition education in primary schools is feasible and successful when vision, support and integration are present. Future research should investigate the vision of the local community and parents on making the combination, and the effects of the combination on pupils and their environment.
The T4bSS of Legionella features a two-step secretion pathway with an inner membrane intermediate for secretion of transmembrane effectors
Authors: Malmsheimer Silke , Grin Iwan , Bohn Erwin , Franz-Wachtel Mirita , Macek Boris , Sahr Tobias , Smollich Fabian , Chetrit David , Meir Amit , Roy Craig , Buchrieser Carmen , Wagner Samuel
Published: November 2024

To promote intracellular survival and infection, Legionella spp. translocate hundreds of effector proteins into eukaryotic host cells using a type IV b protein secretion system (T4bSS). T4bSS are well known to translocate soluble as well as transmembrane domain-containing effector proteins (TMD-effectors) but the mechanisms of secretion are still poorly understood. Herein we investigated the secretion of hydrophobic TMD-effectors, of which about 80 were previously reported to be encoded by L. pneumophila. A proteomic analysis of fractionated membranes revealed that TMD-effectors are targeted to and inserted into the bacterial inner membranes of L. pneumophila independent of the presence of a functional T4bSS. While the T4bSS chaperones IcmS and IcmW were critical for secretion of all tested TMD-effectors, they did not influence inner membrane targeting of these proteins. As for soluble effector proteins, translocation of all investigated TMD-effectors depended on a C-terminal secretion signal. A deeper analysis of the TMD-effector SidF showed that this signal needed to be presented towards the cytoplasmic side of the inner membrane and that a small periplasmic loop was required for efficient translocation. We propose that strongly hydrophobic TMD-effectors are secreted in a two-step secretion process: Initially, an inner membrane intermediate is formed, that is extracted towards the cytoplasmic side, possibly by the help of the type IV coupling protein complex and subsequently secreted into eukaryotic host cells by the T4bSS core complex. Overall, our study highlights the amazing versatility of T4bSS to secrete soluble and TMD-effectors from different subcellular locations of the bacterial cell.
El análisis del discurso político: discurso populista, discursos alternativos y accidentes discursivos. Introducción
Authors: Bolívar Adriana
Published: November 2024

El análisis del discurso político: discurso populista, discursos alternativos y accidentes discursivos. Introducción.
La Retórica Reaccionaria en Acción: Un análisis de las fórmulas de persuasión desplegadas por los críticos del multiculturalismo
Authors: Faúndez García Rocío
Published: November 2024

El artículo tiene por objetivo identificar, desde una mirada teórico-política, cuáles son las principales fórmulas retóricas empleadas por los críticos a las políticas multiculturales centradas en los inmigrantes. Para ello se revisan algunos textos críticos destacados, aplicando el marco de análisis desarrollado por Hirschman (1991), quien identifica tres grandes tesis que articulan en forma casi exhaustiva el discurso de los sectores reaccionarios, en su dimensión persuasiva: la tesis de la perversidad, la tesis de la futilidad y la tesis del riesgo. El análisis muestra que las dos tesis más socorridas por los críticos contemporáneos del multiculturalismo son la tesis de la perversidad (según la cual las políticas multiculturales impactan de forma negativa, precisamente las situaciones que pretendían mejorar: perjudican a los inmigrantes, y producen conflicto intercultural); y la tesis del riesgo (según la cual estas políticas amenazan mortalmente algunos logros fundamentales de las sociedades democráticas: el estado de derecho, la ciudadanía, el Estado de Bienestar, y, en último término, la misma Modernidad y la civilización occidental). Ambas son empleadas, además, de forma conjunta, potenciando así su potencial persuasivo. Desde el punto de vista de su retórica, por tanto, los autores críticos del multiculturalismo se asemejan a representantes del pensamiento reaccionario que, a lo largo de los últimos tres siglos, han procurado resistir en su momento reformas como la introducción del sufragio universal o de sistemas de protección social.
‘Políticamente correcto’: la política de la lengua y la cultura
Authors: Fairclough Norman , Ghio Elsa
Published: November 2024

En este artículo enfoco la polémica acerca de la ‘corrección política’ (CP) en relación con tres cuestiones: una pregunta socio-histórica, una pregunta teórica y una pregunta política. (1) ¿A qué responde el aparentemente creciente interés político por producir cambios sociales y políticos a través de cambios culturales y lingüísticos? ¿Qué ha ocurrido en la cultura que pueda explicar este ‘giro cultural’ y este ‘giro lingüístico’ en la política, en la teoría social y política y en otros dominios de la práctica social? (2) ¿Cómo podemos comprender las relaciones entre la cultura, el lenguaje y otros elementos de la vida social y de las prácticas sociales – cómo tenemos que entender la relación entre el cambio en la cultura y en la lengua y el cambio social? (3) Para quienes están políticamente involucrados con el cambio político y social sustantivo (tanto desde la derecha como desde la izquierda), ¿qué lugar puede ocupar en una política centrada en torno a la cultura y el lenguaje una estrategia política que pueda tener alguna posibilidad de éxito? El artículo concluye con una consideración acerca de las tácticas y estrategias discusión para cuestionar a las críticas de la ‘CP’.
Batallas internas en la comunicación política. Estructura retórica y construcción de los participantes en un discurso de Esperanza Aguirre
Authors: Duque Eladio
Published: November 2024

Se propone un análisis de las estrategias lingüístico-discursivas de construcción de los principales participantes en el discurso que la presidenta de la Comunidad de Madrid pronuncia en el Foro del diario Abc el 7 de abril de 2008. El discurso tuvo una gran repercusión mediática y por él, se anunció que Esperanza Aguirre podría presentarse como candidata a la presidencia del partido en el congreso que el Partido Popular celebraría en julio. Al estudio de los diferentes mecanismos por los que opera la estrategia global de toda ideología (la auto-presentación positiva y la presentación negativa de los grupos ajenos), se le añade las posibilidades persuasivas de sus localizaciones dentro de la estructura retórica del discurso (Mann y Thompson 1992 y Mann 2005). Los resultados confirman la habitual presentación de partidos políticos polarizados. La construcción de los participantes de sectores enfrentados dentro del partido se realiza recurriendo a mecanismos lingüísticos más sutiles. Por otra parte, la importancia persuasiva de la construcción de los participantes se manifiesta en su repercusión en la estructura discursiva. Los resultados muestran cómo sus dinámicas de construcción -los despliegues y transformaciones de los participantes a lo largo del texto-, sostienen la argumentación del discurso.
Análisis del discurso tecnocientífico que construye la muerte encefálica en un manual de coordinación de trasplantes
Authors: Flores-Pons Gemma , Iñiguez-Rueda Lupicinio
Published: November 2024

La muerte encefálica fue definida en 1968 por el ad hoc Committee of the Harvard Medical School to Examine the Definition of Brain Death. Se trata de una conceptualización de la muerte inexistente antes de la introducción de ventiladores mecánicos, a mitad del s XX, y de la evolución de las medidas de resucitación. En este artículo presentamos la muerte encefálica como controversia tecnocientífica y hacemos un análisis retórico y un análisis de la función, la construcción y la variación del discurso que construye la muerte encefálica en un manual de coordinación de trasplantes de la Organización Nacional de Trasplantes (ONT, España). Los resultados elaborados presentan cuatro construcciones distintas de muerte encefálica en uso en el texto: la muerte encefálica como diagnóstico, como consecuencia, como causa y como proceso y estado. La función de estas construcciones es la constitución de la muerte encefálica como objeto factual, a la vez que erige a las profesionales sanitarias especializadas como sujetos conocedores a través de los protocoles establecidos. Se trata de un texto que a pesar de tener una función retórica de consenso, no cierra las discusiones que mantienen la controversia abierta. El interés en el análisis de la controversia de la muerte encefálica reside en su potencial para el estudio de las relaciones de poder que actúan en la regulación de los procesos de salud y enfermedad, centrales en las sociedades actuales.
Methodology for assessing Repligen ProStream® and HyStream® Tangential Flow Filtration Cassettes in an Antibody-Drug-Conjugate Process
Authors:   Sanofi, ADC Conjugation Development, Ha Benjamin ,   13 quai Jules Guesde, 94400 Vitry-Sur-Seine, France, Gulyás Zoltán ,   Repligen, Sr. Field Applications Scientist, DSP | EMEA West/South.
Published: November 2024

Antibody-drug conjugates (ADCs) is a growing field requiring specific handling due to the highly potent drug or highly active pharmaceutical ingredient (HAPI). The generic ADC process is composed of chemical conjugation steps followed by purification (removal of small molecules) and buffer exchange steps through Tangential Flow Filtration (TFF). In this article, we investigate the use of the ProStream® and HyStream® TFF cassettes from Repligen in comparison to reference cassettes in 1) a side-by-side comparison of a standard process and 2) in a challenging process (high load and high concentration factor). We obtained comparable process performance results (processing time and yield) and product quality attributes (including but not limited to Drug-to-Antibody Ratio (DAR), High Molecular Weight (HMW) and Low Molecular Weight (LMW) levels, residual organic solvent and free drug content) as in the reference processes. Thus, this evaluation provides encouraging results to support ProStream and HyStream cassette use in ADC processes.
Chord Painting
Authors: LI Xiaohong
Published: November 2024

Music and painting are the most common chord phenomena, based on the physical and mathematical forms of chords, distributed as chord fields (quantum fields), respectively expressing time, space and their mirror relations, both of which are physical events and spiritual events.

Strings are the spatial (geometric) semantics of chords, and chords are the characteristic spectrum of strings: different chords contain different spatial semantics: major triads = closed chords (contour lines), minor triads = open chords (facet lines), proportional sequence chords = membrane chords (non-linear space), the three types of strings form a spatial field (spatial language) and produce all spatial states and interactions.

Painting is a chord space event, including the natural laws of space and physical and mathematical forms, and is also the observation basis and application field of string theory, which is the main content of this book.
CONCEPTUAL ASPECTS OF ORGANIZING THE OPERATION OF MOBILE COMPONENTS OF ENVIRONMENTAL MONITORING SYSTEMS
Authors:   Southern Scientific Centre of Russian Academy of Sciences, Rostov-on-Don, Russian Federation, Kapustyan S.G. , Melnik E.V.
Published: November 2024

To solve the problems of monitoring large remote areas and regions, it is advisable to use groups of mobile robots as mobile components of monitoring systems. The article discusses conceptual issues of organizing the autonomous functioning of such groups of robots. The effectiveness of solving monitoring problems by a group of mobile robots is largely determined by its composition. The paper proposes an approach to solving the problem of group control of mobile robots and, in particular, solving the subtask of forming the composition of groups of mobile robots for monitoring large areas, based on the principles of self-organization of technical systems, technologies of multi-agent systems and a distributed registry. The study demonstrates that to solve this problem, intelligent multi-agent systems can be used, which include task agents and mobile robot agents. The application of the proposed approach makes it possible to form, if not optimal, then close to optimal, a group composition capable of effectively solving the problems of monitoring large areas.
Derecho procesal de ayer, de hoy y de siempre
Authors: Pérez Daudí Vicente
Published: November 2024

En esta obra se abordan los grandes conceptos del Derecho procesal: Acción. Jurisdicción. Proceso. Juicio Jurisdiccional, analizando los nuevos retos digitales a los que se enfrenta la Justicia. El análisis, en clave actual de los tres grandes conceptos del Derecho procesal era esencial en este tiempo de transición digital, pues todas las obras existentes en el mercado carecen, precisamente, de esta doble cualidad: la modernidad (sin menoscabo de los fundamentos clásicos, también aquí trabajados) y la digitalización. En esta monografía se ofrece el abordaje de los tres conceptos clásicos -Acción, Jurisdicción y Proceso- en un marco de modernidad, digitalización y nueva concepción de la Justicia como “servicio público”.
The Influence of Dynamic Capabilities, Entrepreneurial Orientation, and Quality of Strategy on Increasing Business Performance (Empirical Study on Batik MSMEs in Semarang City)
Authors: Shofwani Siti Aniqoh , Santoso Aprih , Armiella Adestya Ayu , Wahdi Nir Setyo , Nurhidayah Fitriyah
Published: November 2024

This study aims to examine and analyze the effect of dynamic capabilities, entrepreneurial orientation, and strategic quality on improving business performance (Empirical Study on Batik SMEs in Semarang City). The 3,256 MSME actors are the population. The 97 respondents selected were batik MSME actors who were active for a period of 5 years. The dynamic capability variable partially has a positive and significant effect on business performance. This is evidenced by the value of the regression coefficient value of 0.231 (positive) with a significance value of 0.000 <0.05, then H1 is accepted. The entrepreneurial orientation variable partially has a positive and significant effect on business performance. This is evidenced by the regression coefficient value of 0.345 (positive) with a significance value of 0.000 <0.05, then H2 is accepted. The strategic quality variable partially has a positive and significant effect on business performance. This is evidenced by the value of the regression coefficient of 0.179 (positive) with a significance value of 0.014 <0.05, then H3 is accepted. The variables of dynamic capability, entrepreneurial orientation and strategic quality simultaneously affect business performance. This is evidenced by the calculated F value of 14.328 > F table of 3.09 and a significance value (Sig.) 0.000 <0.05, then H4 is accepted.
The Influence of Organizational Culture, and Work Motivation on Employee Performance with Job Satisfaction as an Intervening Variable
Authors: Faturrahman Satria , Yuniawan Ahyar
Published: November 2024

This study investigates the critical link between human capital and organizational success by examining the influence of organizational culture and work motivation on employee performance. We hypothesize that job satisfaction plays a  mediating role in this interplay. Employing a quantitative approach with 90 respondents at Hotel Grandhika Pemuda Semarang, Indonesia, we analyze data using multiple linear regression. Our findings reveal a significant positive influence of both organizational culture and work motivation on job satisfaction. Furthermore, all three variables demonstrate a robust impact on employee performance. Notably, job satisfaction emerges as a significant mediator, underscoring its crucial role in translating positive organizational factors into improved performance. These findings highlight the multifaceted nature of human capital management. To optimize employee performance, organizations like Hotel Grandhika Pemuda Semarang should prioritize fostering a positive organizational culture, nurturing employee motivation, and cultivating a sense of job satisfaction among their workforce.
The Effect of Service Quality, Product Quality, and Pricing on the Level of Customer Satisfaction at PT. IT Matsuoka Winner Industry
Authors: Wulandari Romadona Ayu , Yuniawan Ahyar
Published: November 2024

This study aims to understand the level of customer satisfaction with service quality, product quality, and price at PT. Ti Matsuoka Industry Winner. This study uses variables of service quality, product quality and price as independent variables and consumer satisfaction as the dependent variable. The population in this study used all consumer data of PT. Ti Matsuoka Industry Winner. With a sample of 118 individuals using the purposive sampling method. The data analysis method used in this study is multiple linear regression technique. The results of the analysis show that the variables of service quality, product quality and price have a positive and significant effect on consumer satisfaction at PT. Ti Matsuoka Winner Industry. And in this study price has a dominant influence on consumer satisfaction.
The Influence of Brand Equity, Brand Experience, and Brand Reputation on Brand Loyalty (Study of DANA E-Wallet Application users in Semarang City)
Authors: Pangestika Teta Kirana , Indriani Farida
Published: November 2024

The increasingly rapid development of technology has an impact on the use of e-wallet transactions. E-wallet is an innovation supported by technological developments as a digital wallet that can be accessed easily and cheaply. One e-wallet that is often used by people is DANA. This application provides many benefits to its users such as providing free transfer services and payments to various other platforms. The aim of this research is to determine the influence of brand equity, brand experience and brand reputation on brand loyalty among DANA e-wallet users. The method used in this research is a quantitative method with a sample of 100 respondents. The data analysis method used is multiple linear regression analysis. The results of this research show that brand equity has a significant influence on brand loyalty as evidenced by the calculated t value being greater than the t table (8.011>1.660). Likewise, brand experience has a significant influence on brand loyalty as evidenced by the calculated t value being greater than the t table (4.004>1.660). The influence of independent variables such as brand equity and brand experience simultaneously influences dependent variables such as brand loyalty by 73%, while 27% is influenced by factors not discussed in this research.
Analisis Beban dan Status Pencemaran Sungai Banjir Kanal Timur, Semarang, Segmen Tengah hingga Hilir
Authors: Wahyuni Fidya Ridha , Haeruddin Haeruddin , Rahman Arif
Published: November 2024

Sungai Banjir Kanal Timur (BKT) merupakan salah satu sistem drainase di Semarang. BKT melintasi pemukimanpadat penduduk, daerah industri mikro, serta hilirnya dijadikan tempat sandar dan transportasi kapal nelayan.Hal tersebut menghasilkan limbah yang berpotensi masuk ke sungai dan mempengaruhi kualitas air sungai.Tujuan penelitian ini untuk mengetahui konsentrasi TSS, BOD, dan COD sebagai variabel utama penelitian,mengetahui beban dan status pencemaran, serta rasio BOD/COD Sungai Banjir Kanal Timur. Penelitiandilaksanakan pada Februari-Maret 2024. Metode yang digunakan adalah purposive sampling berdasarkan sumberpencemar. Sampel diambil di 3 stasiun, 3 titik, dengan 3 kali pengulangan. Pengukuran secara insitu dilakukanuntuk variabel suhu, pH, debit sungai, salinitas, dan DO. Pengukuran TSS dan BOD dilakukan di LaboratoriumPengelolaan Sumber Daya Ikan dan Lingkungan (PSDIL), Departemen Sumber Daya Akuatik, UniversitasDiponegoro. Pengukuran COD dilakukan di Laboratorium Teknik Lingkungan, Universitas Diponegoro. Hasilpenelitian pada stasiun I-III menunjukkan bahwa konsentrasi TSS berkisar antara 12-48 mg/L, konsentrasi BODberkisar antara 7,9-43,4 mg/L, dan konsentrasi COD berkisar antara 45,68-97,26 mg/L. Sungai Banjir Kanal Timurmenghasilkan beban pencemaran TSS, BOD, dan COD secara berurutan sebesar 487,30-18.035,14 kg/hari,375,41-37.272,61 kg/hari, dan 2.368,05-43.756,68 kg/hari. Sungai Banjir Kanal Timur telah tercemar kategoricemar ringan dengan nilai Indeks Pencemaran berkisar antara 2,98-3,86. Polutan di Sungai Banjir Kanal Timurbersifat non-biodegradable pada stasiun I dan II, dan diperlukan treatment pada stasiun III dengan RasioBOD/COD berkisar antara 0,23-0,38.
ITB Synthetic Unit Hydrograph Method with Exact and Numerical Peak Discharge Factor (Kp) and Normalized Unit Rainfall Duration (Tr)
Authors: Natakusumah Dantje Kardana
Published: November 2024

The Synthetic Unit Hydrograph (SUH) method is a very important method in water resource planning and flood discharge analysis in river basins This method is very useful, especially for flood simulations in river basins that experience changes in land use. However, The HSS method generally has shortcomings, including difficulties in explaining the origin of peak discharge formulas and inaccuracies in comparing hydrograph volumes with effective rainfall. Additionally, there are challenges in adapting the method for different rainfall durations, and only a few methods can be calibrated. To overcome these challenges, the ITB SUH method has been developed with several innovative features. This method follows the mass conservation principle and simplifies the process of deriving peak discharge formulas, making it an effective educational tool. The ITB method combines exact and numerical approaches in calculating the Peak Discharge Factor (Kp), improving the accuracy of peak discharge estimation, which is very important in flood management. This method also introduces the Normalized Unit Rainfall Duration (Tn), a dimensionless factor that provides flexibility in adjusting the hydrograph duration, making it easier to convert to shorter durations. In addition, the ITB method includes a calibration mechanism that allows users to adjust the hydrograph with measurement values. The ITB SUH method's innovative features have been successfully demonstrated in various cases, yielding highly positive outcomes.
Seasonal variation in dietary intake and its association with obesity-related chronic diseases in northeast China
Authors: Wang Cheng , Li Zican , Guan Dongwei , Fu Hongxin , Feng Rennan
Published: November 2024

Objective

The objective of this study was to assess seasonal changes in dietary and nutrient intake of residents (18-75 years old) in Northeast China during summer and winter, and to explore the associations between fatty acids, phytosterols, and the prevalence of obesity-related chronic diseases, particularly obesity, hyperlipidemia, and NAFLD.

Methods

A total of 4773 participants from the Internet-based Dietary Questionnaire for Chinese (IDQC) were included in this study. Dietary intake information was collected using a validated food frequency questionnaire. Student’s t-test or Mann-Whitney U-test was used to analyze continuous variables, while Chi-squared tests were used to compare categorical variables. Multivariable logistic regression was employed to assess the relationship between fatty acids, phytosterols, and obesity-related chronic diseases.

Results

The mean consumption of legumes, vegetables, fruits, nuts, dairy products, fish, condiments, energy, protein, fat, and carbohydrate differed significantly between summer and winter (P < 0.05). Significant inverse associations were found between both fatty acids and phytosterols and obesity-related chronic diseases in multivariate adjusted models. Summer polyunsaturated fatty acid (PUFA) intake was negatively associated with the prevalence of hyperlipidemia (Q4, OR, 0.515; 95%CI, 0.283-0.921; P < 0.05) and non-alcoholic fatty liver disease (NAFLD) (Q4, OR, 0.331; 95%CI, 0.176-0.599; P < 0.001). Phytosterols intake was negatively associated with the prevalence of obesity (Q4, OR, 0.603; 95%CI, 0.414-0.873; P < 0.05), hyperlipidemia (Q4, OR, 0.420; 95%CI, 0.233-0.731; P < 0.001), and NAFLD (Q4, OR, 0.206; 95%CI, 0.111-0.360; P < 0.001) during the summer.

Conclusions

Higher PUFA intake was associated with a lower prevalence of obesity, hyperlipidemia, and NAFLD. Phytosterol intake was inversely associated with the prevalence of hyperlipidemia and NAFLD. These findings suggest that the associations between PUFA and phytosterols and the prevalence of obesity-related chronic diseases may be influenced by seasonal differences in food intake.
LCN2 aggravates diabetic cataracts by promoting ferroptosis in lens epithelial cells
Authors: Zhang Jiayue , Sun Liyao , Yu Xiaohan , Yang Chen , An Qi , Wei Chaoqun , Ge Hongyan
Published: November 2024

Background

Cataracts are the leading cause of reversible blindness worldwide. Diabetic cataract (DC), a prevalent complication of diabetes mellitus, is characterized by its high occurrence, rapid progression, and severe impact. The prevalence of diabetes varies greatly between the northern and southern regions, with higher rates observed among northern residents. DC-induced lens opacity is mainly attributed to oxidative stress. However, it remains unclear whether ferroptosis, a form of regulated cell death, occurs in crystalline epithelial cells during the pathogenesis, which may represent a novel mechanism contributing to DC.

Methods

Transmission electron microscopy, quantitative assays for iron levels and reactive oxygen species (ROS), real-time quantitative polymerase chain reaction (RT-qPCR), western blotting, immunofluorescence, and immunohistochemistry were used to detect ferroptosis. Gene editing techniques were utilized to study the regulatory relationships among lipocalin 2 (LCN2), glutathione peroxidase 4 (GPX4), and ferritin heavy chain (FTH). Local knockdown of the LCN2 gene in B-3 cells and the eyes of Sprague Dawley (SD) rats was performed to verify and further explore the role and regulatory mechanisms of LCN2 in DC-associated ferroptosis.

Results

An in vitro model using high glucose levels and an in vivo model with streptozotocin-induced diabetes in SD rats were successfully established. Ferroptosis was observed in both in vitro and in vivo experiments. LCN2 protein was normally expressed in human and rat lens epithelial cells, but its expression significantly increased during ferroptosis. The ferroptosis inhibitor, ferrostatin-1 (Fer-1) effectively inhibited ferroptosis and reduced LCN2 protein expression. Notably, local knockdown of LCN2 via gene editing protected lens epithelial cells from ferroptosis in vitro and slowed the progression of DC in SD rats in vivo.

Conclusion

Our findings underscore the significant role of ferroptosis in the pathogenesis of DC, suggesting that selectively targeting LCN2 activation and enhancing ferroptosis resistance may offer a novel therapeutic approach for treating DC.
Experimental research on mechanically and thermally activation of nano-kaolin to improve the properties of ultra-high-performance fiber-reinforced concrete
Authors: Althoey Fadi , Zaid Osama , Yasir Muhammad , Abuhussain Mohammed Awad , Dodo Yakubu , Mohamed Abdullah
Published: November 2024

The rising demand for ultra-high-performance concrete (UHPC) necessitates innovations in sustainable materials. This study explores the substitution of ordinary Portland cement (OPC) with thermally and mechanically activated nano-kaolin in varying proportions from 0.5 to 0.25%. A uniform quantity of double-hooked end steel fibers was added to all the mixes. Activated nano-kaolin variants showed significant enhancement in UHPC properties. Specifically, UHPC with 0.20% thermally activated kaolin (B3-TAK-20) exhibited a 21.6% increase in compressive strength and a 25.5% increase in modulus of elasticity at 90 days, with the modulus of rupture doubling compared to the reference mix. These improvements are attributed to the amorphous nature of thermally activated nano-kaolin, resulting in a denser concrete matrix and reduced porosity. Beyond the optimal 0.20% kaolin replacement, an increase to 0.25% diminished compressive strength. Durability tests showed enhanced acid resistance, with only a 6.7% mass loss for the thermally activated nano-kaolin mix and a consistent reduction in water absorption by 14.4% as kaolin proportions increased from 0.5 to 0.25%. The study also noted a decrease in water absorption by 22.9 and 12.3% at 56 and 90 days, respectively, indicating the thermally activated nano-kaolin’s enhanced performance. This research underscores the potential of activated kaolin as a viable alternative to OPC, paving the way for more sustainable UHPC production.
Effect of Alkalization Time on the Toughness and Strength of Jute Sack Waste Lamina Composite as an Alternative Car Bumper Material
Authors: Yudiono Heri , Parlaungan Siregar Januar , Asri Sarwi , Hidayat Hanif , Chikam David Badi’ul , Aprilian Johan Andric , Supriyadi Toni Agung , Cionita Tezara , Fitriyana Deni Fajar
Published: November 2024

Alkaline conditions, such as the concentration of Sodium Hydroxide (NaOH) and the duration of soaking time, significantly affect the mechanical properties of natural fiber and its composites. The objective of this study is to investigate the effect of alkali treatment on the impact toughness and tensile strength of laminated composites made from jute sack (burlap) waste woven fibers, which can potentially be used as a substitute material for vehicle bumpers. The experimental group comprised specimens that were alkalized by manipulating the duration of soaking and the concentration of alkali in the woven fibers of jute sack waste, with a fiber orientation pattern of 0°/+90°/0°/+90°/0°. The fiber immersion time is 24 hours, 48 hours, and 72 hours when using a 5% NaOH concentration. Epoxy resin serves as a composite matrix, specifically epoxy resin and epoxy hardener. Two sets of specimens underwent tests to measure their tensile and impact strengths. The result of the study reveals that the jute sack laminated epoxy composite with a fiber orientation of 0°/+90°/0°/+90°/0° had the maximum tensile strength and impact strength after a 48-hour alkaline treatment at 14.99 MPa and 0.010 J/mm2, respectively. The alkali treatment has successfully enhanced the tensile strength of the jute fiber by approximately 42.49% and 39.66% when compared to the untreated jute fiber. The study concludes that the utilization of alkaline process with NaOH improves the surface area of the fiber, compatibility with polymer matrix and mechanical strength.
Effects of expanded polytetrafluoroethylene porous membrane covering and biochar on nitrogen, phosphorus, and potassium contents in aerobic composting
Authors: Cao Jie , Li Ruirong , Qu Haoli , Wang Pengjun , Fu Jingjing , Gong Yan , Chen Mingjiang
Published: November 2024

The effects of covering technology and the combined membrane covering with biochar on nutritional elements content during the aerobic composting process of agricultural waste were studied. The results showed that the concentration effect of organic matter degradation during composting increased the nitrogen, phosphorus, and potassium contents in all treatment groups. The proportion of available phosphorus and available potassium also increased to varying degrees. Compared with composting without an expanded poly(tetrafluoroethylene) (PTFE) covering with 0.2 to 0.4 µm pores, the covered composting increased the total nitrogen, total phosphorus, total potassium, available phosphorus, and available potassium contents by 13.1%, 12.6%, 7.4%, 21.5%, and 16.6%, respectively, while the addition of biochar to polymer membrane-covered composting increased the total nitrogen, total phosphorus, total potassium, available phosphorus, and available potassium contents by 19.7%, 13.7%, 8.3%, 18.0%, and 20.2%, respectively. This study indicated that membrane covering technology can effectively increase the nutrient element contents in compost products and the availability of phosphorus and potassium and that the addition of biochar enhances these effects.
Studia Humanitatis – Ars Hermeneutica. Metodologie a theurgie hermeneutické interpretace X
Authors: Slivka Daniel , Bernacki Marek , Kapsová Eva , Ševčík Mimloš , Kotowski Bartłomiej , Jankechová Katarína , Blahutková Daniela , Sommerová Adéla , Vorel Jan , Kalnická Zdeňka , Mikulášek Miroslav
Published: November 2024

The dominants of the aesthetic and philosophic world of Belyj’s work rest on the effort to express the aiming to the Light, to the spiritual integrity, to the interconnection of the Macrocosm with the Microcosm, thus Belyj can be considered as a cosmic poet. All the world happening appears as a “cosmic cognitive act”, the act of creation by means of human cognitive activity of the New Cosmos in the frame of the present one. The history of the human culture forming is always seen as a gradual streaking of the “new light” into the human consciousness; the light that will re-integrate the human and its culture – whereas so far it had been tragically cleft in “head and soul” (the West and the East) – into one harmonic unity, a real organism. Although the tragic cleaving is, according to Belyj, still lasting, it is possible to discover the still-living spirit of the synthesis. The way to the spirit – the light consists of the creation of the real Cosmos. Thus it becomes a result of a creative soul. We can notice that in Belyj’s theories a “cult of the light” is in play. The light often, also in the novel Petersburg, stands for “the light of Christ”, penetrating the souls of the characters. Or it represents “the divine light”, being present at the birth of the Cosmos. In conclusion, Light symbolizes the re synthesis, the return of the human to the spiritual fundaments.
Soil organic carbon storage in different agroforestry systems associated with Coffee in Nariño, Colombia
Authors: Delgado-Vargas Ivan A. , Benavides Franco Natalia
Published: November 2024

Introduction. Anthropogenic activities are one of the causes of the increase in global temperature, which affects agro-environmental, ecological and socioeconomic processes. The contributions of agricultural production systems in the capture and storage of greenhouse gases have been little studied. Still, the literature indicates that the inclusion of trees on farms is an option to capture and store some greenhouse gases. Objective. To evaluate the relationship between floristic and structural diversity of coffee production systems and soil organic carbon storage (SOC). Materials and methods. The study was carried out during the period 2019-2020, in three localities Sandoná, San Pablo and Buesaco, Nariño-Colombia. Twelve agroecosystems with coffee were visited, where Shannon-Weiner and Simpson indices, tree density and complete soil analysis with samples at 30 cm depth were evaluated. An analysis of variance and principal components was sufficient for the analysis of the information. Results. The SOC was  not presented statistical differences (p>0.05), which ranged between 38.55 Mg ha-1 in the full sun coffee arrangement in Buesaco and 96.63 Mg ha-1 in the coffee arrangement with miscellaneous in the same municipality, which presented the highest species diversity. Conclusions. It was proved  that the coffee harvest associated with agroforestal systems (T3 and T4) with higher diversity and abundance of species, is higher than   carbon accumulated in the soil compared to the monoculture arrangement (80,43 Mg ha-1 vs 58,32 Mg ha-1, severally), this diversification give to the family and enviroment some services and goods, contributing to the mitigation of climate change.