bluuebunny/crossref_metadata_2025 huggingface dataset:
Air Quality and Atmospheric Emissions from the Operation of the Main Mexican Port in the Gulf of Mexico from 2019 to 2020
Authors: González Rivera Ana Isabel , Mugica-Álvarez Violeta , Sosa Echeverría Rodolfo , Sánchez Álvarez Pablo , Magaña Rueda Víctor , Vázquez Cruz Gustavo , Retama Armando
Published: January 2023

Pollutant emissions into the atmosphere derived from port activities can be transported to surrounding regions and cities depending on wind speed and direction, having an impact on air quality. In this research, emissions of atmospheric pollutants (NOx, CO, NMHCs, CO2, SO2, TSP, PM2.5 and PM10) were estimated for: tanks, container, roll-on/roll-off (RO-RO), bulk carriers and general cargo ships, using emission factors in the hoteling and maneuvering stage in the port area of Veracruz, Mexico, during 2019 and 2020 despite the suspension period of activities due to the SARS-CoV-2/COVID-19 pandemic. Among the total estimated emissions, CO2 presented the highest values for 2019 (31,177 kg/year) and 2020 (29,003 kg/year), whereas CH4 presented the lowest values with 0.294 kg/year for 2019 and 0.273 kg/year for 2020. The highest estimated emissions for CO2, NOx and SO2 occurred in the maneuvering stage in 2019 for bulk carriers, tanks and container ships. Likewise, the highest estimated emissions were during the hoteling stage of the container ships in 2020. This study will provide an updated ship emissions inventory for the Gulf of Mexico region where the Port of Veracruz is located. In addition, SO2 and PM2.5 measurements were performed from October 2019 to December 2020. PM2.5 concentrations exceeded the Mexican Ambient Air Quality Standard (MAAQS) value of 45 µg m−3 for the 24-h average concentration several times, on the opposite, SO2 exhibited concentrations up to 20 times lower than the 24-h MAAQS value of 40 ppb. Results showed that pollutant emissions in the port of Veracruz exhibited a seasonal variability, modifying their dispersion and the possible effects. Our main conclusion is that current port area is the major source of pollutant emissions (SO2 and PM2.5) throughout the year, whereas the expansion area of the port of Veracruz does not represent still a significant rise of pollutant emissions, but it is expected that the growth of port activity will directly increase the concentrations of pollutants emitted.
Progress on the Impact of Persistent Pollutants on Marine Turtles: A Review
Authors: Arienzo Michele
Published: January 2023

The review examines the most recent advances on the effect of persistent pollutants on sea turtles. Research on this topic is still limited, especially that related to toxicity, since they are protected species and in vivo toxicity studies are difficult, with most studies carried out on deceased individuals. Besides toxicology, the review considers direct links to pollution sources, reproduction impact, health effects and biomarkers of pollution exposure. Little attempts have been made so far to gather data that would provide insight into the causes of the observed health trends. Considering this, studies correlating PPs accumulation with health parameters were also discussed. The review synthesizes the recent progress of the research on these topics and indicates the main urgent need of investigation to limit threats from anthropic pressure.
Long-Term Kinetics of Serum Galactomannan during Treatment of Complicated Invasive Pulmonary Aspergillosis
Authors: Tragiannidis Athanasios , Linke Christina , Correa-Martinez Carlos L. , Herbrüggen Heidrun , Schaumburg Frieder , Groll Andreas H.
Published: January 2023

Several studies have evaluated the serum galactomannan (GM) antigen assay in pediatric patients, and there is convincing evidence for its usefulness as a diagnostic tool for invasive Aspergillus infections in patients with acute leukemias or post allogeneic hematopoietic cell transplantation (HCT). Less is known about the utility of the assay in monitoring responses to treatment in patients with established invasive aspergillosis (IA). Here, we present the long-term kinetics of serum galactomannan in two severely immunocompromised adolescents with invasive pulmonary aspergillosis (IPA) who were cured after complicated clinical courses. We also review the utility of the GM antigen assay in serum as a prognostic tool around the time of diagnosis of IA and as a biomarker to monitor disease activity in patients with established IA and assess responses to systemic antifungal therapy.
Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
Authors: Martin-Souto Leire , Antoran Aitziber , Areitio Maialen , Aparicio-Fernandez Leire , Martín-Gómez María Teresa , Fernandez Roberto , Astigarraga Egoitz , Barreda-Gómez Gabriel , Schwarz Carsten , Rickerts Volker , Hernando Fernando L. , Rementeria Aitor , Buldain Idoia , Ramirez-Garcia Andoni
Published: January 2023

The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium/Lomentospora in patients with CF.
Maintaining Agricultural Production by Building Local Distribution Systems in the Northern Area of Japan
Authors: Kawasaki Noriaki , Washio Tamaki , Nakamura Katsunori , Nagahama Ken-Ichiro
Published: January 2023

In the field of vegetable farming, it has become a common approach for farmers to advance into the secondary and tertiary industries to increase their income, an initiative known as the sixth industrialization. Under these circumstances, a growing trend is to outsource a part of the sixth industrialization activities in order to improve consumer satisfaction, strengthen market competitiveness, and avoid investment risks. However, owing to a mismatch between farmers and processors, there are few cases that result in collaboration. Under such circumstances, a new distribution channel called local distribution systems have been born, and its importance is increasing in Japan. This paper demonstrates how a local distribution system for farmers living in rural areas could address this distortion. The concept of local distribution systems has been used since the 1990s, and yet, its significance and importance are still increasing in relevancy in today’s Japanese agriculture. In this study, the subject is an intermediary (Company A) that originated from farmers, so it was able to understand the behavioral principles of farmers and to identify businesses that could not be covered by the management resources of farmers themselves. Through the entrustment of the business, company A could support the production and sales activities of the farmers. The following conclusions were drawn: (1) the company does not directly involve members in the decision-making of sales methods but instead provides a number of options for decision-making, and (2) the needs on the production side will match those on the consumer side and play the role of communication. By building such a collaboration system, the company succeeded in establishing a local distribution system. In the distribution of vegetables, which is characterized as perishable items, it is essential to pursue efficiency and rationality through a wholesale market system to distribute the products from producers to a large number of consumers. However, constraints in the wholes system limit the extent to which this local distribution functions. This paper demonstrates how a local distribution system for farmers living in rural areas could address this distortion. The concept of local distribution systems has been used since the 1990s, yet its significance and importance are still increasing in relevancy in today’s Japanese agriculture.
Urban Distribution and Evolution of the Yangtze River Economic Belt from the Perspectives of Urban Area and Night-Time Light
Authors: Xu Huimin , Hu Shougeng , Li Xi
Published: January 2023

Research on urban development patterns and urban sprawl in the Yangtze River Economic Belt (YREB) has received wide attention. However, existing research has always made use of statistical data, which are not often available. Considering the high availability of satellite data, this study attempts to combine two satellite-acquired indexes, including urban area and night-time light, to evaluate the urban development of the YREB during 2012–2019. The methods included using growth index, rank-size law, and the Markov transition matrix, as well as constructing urban night-time light density and unbalanced index of night-time light, derived from the Gini Index. Some important patterns were revealed. Firstly, the three reaches (Upper Reaches, Middle Reaches, and Lower Reaches) in the YREB have all shown rapid growth in urban area and night-time light, and they all have increased in urban density. Secondly, from the perspective of regional disparity, the Upper Reaches have the highest growth rate of the urban area, while the Middle Reaches have the highest growth rate of night-time light; and the Upper Reaches have more urban sprawl, while the Middle Reaches have shown more compact growth. Thirdly, higher urban density is related to more balanced development across cities. Our study suggests new knowledge can be obtained by combining the two indexes for understanding urban development in the YREB.
Exploring the Spatial–Temporal Variation in Cultivated Land Quality and Influential Factors in the Lower Reaches of the Yangtze River from 2017 to 2020
Authors: Wang Zixuan , Fei Xufeng , Sheng Meiling , Xiao Rui
Published: January 2023

Cultivated land quality is directly related to national food security; hence, it is necessary to determine the spatial–temporal characteristics and factors that influence its variation. This study analyzed the soil properties and cultivated land quality in the Hang-Jia-Hu Plain, the most important grain production base in Zhejiang Province, located in the lower reaches of the Yangtze River, from 2017 to 2020. In addition, the factors that influenced cultivated land quality variation were explored. The results showed that soil pH and soil organic matter (SOM) significantly improved from 6.32 and 29.07 g/kg in 2017 to 6.38 and 31.54 g/kg in 2020, whereas the variations in available phosphorus (AP) and available potassium (AK) were not significant. More than 60% of the cultivated land still had the potential for soil nutrient status improvement. The cultivated land quality indicator (CLQI) calculated based on the national standard (GB/T 33469-2016) significantly increased from 0.90 in 2017 to 0.91 in 2020. According to the CLQI classification, approximately three quarters of the cultivated land was defined as high-yielding fields. Although the spatial pattern for CLQI was similar between 2017 and 2020, more than 75% of the cultivated land quality showed an increasing trend that was mainly located in the northeastern and central areas. The results of influential factor detection indicated that the improvement in SOM and available soil nutrients including AP and AK was the main reason for the CLQI increase, whereas the climate, topography, and socioeconomic factors had little influence on the change in CLQI. In addition, when influential factors interacted, a significant increase in the explanatory ability for CLQI was obtained, especially for the interaction of SOM and AP variation, which explained 41% of the CLQI variation. This study provides basic foundations and references for cultivated land quality monitoring and improvement in the lower reaches of the Yangtze River, China.
Laser Ablated Albumin Functionalized Spherical Gold Nanoparticles Indicated for Stem Cell Tracking
Authors: Dziedzic Dilcele Silva Moreira , Mogharbel Bassam Felipe , Irioda Ana Carolina , Stricker Priscila Elias Ferreira , Woiski Thiago Demetrius , Machado Thiago Neves , Bezerra Jr Arandi Ginane , Athayde Teixeira de Carvalho Katherine
Published: January 2023

Cell tracking in cell-based therapy applications helps distinguish cell participation among paracrine effect, neovascularization, and matrix deposition. This preliminary study examined the cellular uptake of gold nanoparticles (AuNPs), observing cytotoxicity and uptake of different sizes and AuNPs concentrations in Adipose-derived stromal cells (ASCs). ASCs were incubated for 24 h with Laser ablated Albumin functionalized spherical AuNPs (LA-AuNPs), with average sizes of 2 nm and 53 nm in diameter, in four concentrations, 127 µM, 84 µM, 42 µM, and 23 µM. Cytotoxicity was examined by Live/Dead assay, and erythrocyte hemolysis, and the effect on the cytoskeleton was investigated by immunocytochemistry for β-actin. The LA-AuNPs were internalized by the ASCs in a size and concentration-dependent manner. Clusters were observed as dispersed small ones in the cytosol, and as a sizeable perinuclear cluster, without significant harmful effects on the cells for up to 2 weeks. The Live/Dead and hemolysis percentage results complemented the observations that the larger 53 nm LA-AuNPs in the highest concentrated solution significantly lowered cell viability. The demonstrated safety, cellular uptake, and labelling persistency with LA-AuNPs, synthesized without the combination of chemical solutions, support their use for cell tracking in tissue engineering applications.
Investigations for Material Tracing in Selective Laser Sintering: Part Ι: Methodical Selection of a Suitable Marking Agent
Authors: Eggers Tom , von Lacroix Frank , van de Kraan Fridolin , Reichler Ann-Kathrin , Hürkamp André , Dröder Klaus
Published: January 2023

Selective laser sintering (SLS) with polymers is currently at the transition stage for the production of functional components and holds great potential to revolutionize conventional production processes. Nevertheless, its application capability is confronted by newly imposed requirements regarding reliability and reproducibility. To safeguard these requirements, a deeper process understanding of material aging mechanisms in polymeric materials is needed. In order to enable the traceability of the materials as well as the identification of defective components with subsequent tracing of the cause, the use of a material marking process represents an alternative. SLS in combination with material marking is proving to be an efficient option for reproducible, high-quality manufacturing based on an increased understanding of the process. In this study, the idea of a marker-based traceability methodology for the purpose of process optimization is presented. Fundamental to the subsequent experimental investigation of the marking agent suitability, this work first focuses on the systematic selection of a suitable marking agent for use in SLS. Based on an analysis of the sinter material to be marked and a set of marking technologies, as well as using the selection methodology, the modified polymer marking technology was evaluated as the most suitable marking technology.
Formamidinium Lead Iodide Perovskite Thin Films Formed by Two-Step Sequential Method: Solvent–Morphology Relationship
Authors: Cimrová Věra , Guesmi Mariem , Eom Sangwon , Kang Youngjong , Výprachtický Drahomír
Published: January 2023

Thin films made of formamidinium lead iodide (FAPbI3) perovskites prepared by a two-step sequential deposition method using various solvents for formamidinium iodide (FAI) - isopropanol, n-butanol and tert-butanol, were studied with the aim of finding a correlation between morphology and solvent properties to improve film quality. They were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) and their photophysical properties were studied by means of absorption and photoluminescence (PL) spectroscopies. XRD patterns, absorption and PL spectra proved α-phase formation for all selected solvents. An excessive amount of PbI2 found in perovskite films prepared with n-butanol indicates incomplete conversion. Thin film morphology, such as grain and crystallite size, depended on the solvent. Using tert-butanol, thin films with a very large grain size of up to several micrometers and with preferred crystallite orientation were fabricated. The grain size increased as follows: 0.2–0.5, 0.2–1 and 2–5 µm for isopropanol, n-butanol and tert-butanol, respectively. A correlation between the grain size and viscosity, electric permittivity and polarizability of the solvent could be considered. Our results, including fabrication of perovskite films with large grains and fewer grain boundaries, are important and of interest for many optoelectronic applications.
Generation of Boxes and Permutations Using a Bijective Function and the Lorenz Equations: An Application to Color Image Encryption
Authors: Silva-García Víctor Manuel , Flores-Carapia Rolando , Cardona-López Manuel Alejandro , Villarreal-Cervantes Miguel Gabriel
Published: January 2023

Some images that contain sensitive information and travel through the network require security. Therefore, a symmetric cryptosystem that encrypts images and resists known attacks is developed. Subsequently, in this work, an encryption algorithm known as Image Cipher utilizing Lorenz equation and a Bijective Function—ICLEBF are proposed. In the proposal, the Lorenz equations and the Bijective function are used to generate boxes, the permutation, and schedule keys, considering that all these elements are different in each encryption process. The encryption procedure consists of 14 rounds, where a different box is applied in each round. In this type of algorithm, the impact of quantum computers will be less forceful and can be useful for that epoch. On the other hand, the quality of the encrypted images and the loss of sharpness in decoded images with damage are measured. In addition, an attack from five types of noise (one of which is a developed proposal) is carried out by applying it to encrypted images. Finally, the results of the proposed ICLEBF are compared with other recent image encryption algorithms, including the Advanced Encryption Standard. As a result, this proposal resists known attacks and others that the current standard does not support.
When Fairness Meets Consistency in AHP Pairwise Comparisons
Authors: Dodevska Zorica , Radovanović Sandro , Petrović Andrija , Delibašić Boris
Published: January 2023

We propose introducing fairness constraints to one of the most famous multi-criteria decision-making methods, the analytic hierarchy process (AHP). We offer a solution that guarantees consistency while respecting legally binding fairness constraints in AHP pairwise comparison matrices. Through a synthetic experiment, we generate the comparison matrices of different sizes and ranges/levels of the initial parameters (i.e., consistency ratio and disparate impact). We optimize disparate impact for various combinations of these initial parameters and observed matrix sizes while respecting an acceptable level of consistency and minimizing deviations of pairwise comparison matrices (or their upper triangles) before and after the optimization. We use a metaheuristic genetic algorithm to set the dually motivating problem and operate a discrete optimization procedure (in connection with Saaty’s 9-point scale). The results confirm the initial hypothesis (with 99.5% validity concerning 2800 optimization runs) that achieving fair ranking while respecting consistency in AHP pairwise comparison matrices (when comparing alternatives regarding given criterium) is possible, thus meeting two challenging goals simultaneously. This research contributes to the initiatives directed toward unbiased decision-making, either automated or algorithm-assisted (which is the case covered by this research).
Secondary Metabolites with Anti-Inflammatory Activity from Laurencia majuscula Collected in the Red Sea
Authors: Tammam Mohamed A. , Daskalaki Maria G. , Tsoureas Nikolaos , Kolliniati Ourania , Mahdy Aldoushy , Kampranis Sotirios C. , Tsatsanis Christos , Roussis Vassilios , Ioannou Efstathia
Published: January 2023

The chemical investigation of the organic extract of the red alga Laurencia majuscula collected from Hurghada reef in the Red Sea resulted in the isolation of five C15 acetogenins, including four tricyclic ones of the maneonene type (1–4) and a 5-membered one (5), 15 sesquiterpenes, including seven lauranes (6–12), one cuparane (13), one seco-laurane (14), one snyderane (15), two chamigranes (16, 17), two rearranged chamigranes (18, 19) and one aristolane (20), as well as a tricyclic diterpene (21) and a chlorinated fatty acid derivative (22). Among them, compounds 1–3, 5, 7, 8, 10, 11 and 14 are new natural products. The structures and the relative configurations of the isolated natural products have been established based on extensive analysis of their NMR and MS data, while the absolute configuration of maneonenes F (1) and G (2) was determined on the basis of single-crystal X-ray diffraction analysis. The anti-inflammatory activity of compounds 1, 2, 4–8, 10, 12–16, 18 and 20–22 was evaluated by measuring suppression of nitric oxide (NO) release in TLR4-activated RAW 264.7 macrophages in culture. All compounds, except 6, exhibited significant anti-inflammatory activity. Among them, metabolites 1, 4 and 18 did not exhibit any cytostatic activity at the tested concentrations. The most prominent anti-inflammatory activity, accompanied by absence of cytostatic activity at the same concentration, was exerted by compounds 5 and 18, with IC50 values of 3.69 μM and 3.55 μΜ, respectively.
Could the Microbiota Be a Predictive Factor for the Clinical Response to Probiotic Supplementation in IBS-D? A Cohort Study
Authors: Marchix Justine , Quénéhervé Lucille , Bordron Philippe , Aubert Philippe , Durand Tony , Oullier Thibauld , Blondeau Claude , Ait Abdellah Samira , Bruley des Varannes Stanislas , Chaffron Samuel , Coron Emmanuel , Neunlist Michel
Published: January 2023

Background: Increasing evidence suggests the beneficial effects of probiotics in irritable bowel syndrome (IBS), but little is known about how they can impact the gut microbiota. Our objective was to evaluate the effects of a multistrain probiotic on IBS symptoms, gut permeability and gut microbiota in patients with diarrhoea-predominant IBS (IBS-D). Methods: Adults with IBS-D were enrolled in an open-label trial to receive a multistrain probiotic for 4 weeks. Abdominal pain, stool frequency, quality of life, gut permeability, and the luminal and adherent microbiota from colonic biopsies were evaluated before and after supplementation. Results: Probiotics significantly improved symptoms and quality of life, despite having no impact on permeability in the global population. In the population stratified by the response, the diarrhoea responders displayed reduced colonic permeability after supplementation. The luminal and adherent microbiota were specifically altered depending on the patients’ clinical responses regarding pain and diarrhoea. Interestingly, we identified a microbial signature in IBS-D patients that could predict a response or lack of response to supplementation. Conclusions: The multistrain probiotic improved the symptoms of IBS-D patients and induced distinct effects on the gut microbiota according to the patient’s clinical response and initial microbiota composition. Our study further supports the need to develop individualised probiotic-based approaches regarding IBS.
Genomic Analysis and In Vitro Investigation of the Hop Resistance Phenotype of Two Novel Loigolactobacillus backii Strains, Isolated from Spoiled Beer
Authors: Kiousi Despoina Eugenia , Bucka-Kolendo Joanna , Wojtczak Adrian , Sokołowska Barbara , Doulgeraki Agapi I. , Galanis Alex
Published: January 2023

Loigolactobacillus backii is an important beer-spoiling species, exhibiting high hop tolerance. Here, we present the annotated whole genome sequence of two recently isolated strains, Lg. backii KKP 3565 and KKP 3566. Firstly, to study the genetic basis of the persistence of the two isolates in beer, a comprehensive bioinformatic analysis ensued. Their chromosome map was constructed, using whole-genome sequencing and assembly, revealing that the two strains carry genomes with a length of 2.79 Mb with a GC content of 40.68%. An average nucleotide identity (ANI) analysis demonstrated that the novel strains possess unique genomic sequences, also confirming their classification into the Lg. backii species. Their genome harbors numerous insertion sequences and plasmids, originating from other beer-spoiling species. Regarding their adaptation in brewery environment, homologous genes that confer resistance to hop were spotted, while the impact of hop bitters and pure beer on bacterial growth was investigated, in vitro. In brief, low hop concentrations were found to induce the proliferation of strains, while a higher concentration negatively affected their growth. Nonetheless, their ability to survive in pure beer indicated their tolerance to high hop concentrations. These results offer insight into the capacity of Lg. backii KKP 3566 and Lg. backii KKP 3566 to tolerate the extreme conditions prevalent in the brewery environment.
Comparing the Efficacy of MALDI-TOF MS and Sequencing-Based Identification Techniques (Sanger and NGS) to Monitor the Microbial Community of Irrigation Water
Authors: Surányi Botond Bendegúz , Zwirzitz Benjamin , Mohácsi-Farkas Csilla , Engelhardt Tekla , Domig Konrad J.
Published: January 2023

In order to intensify and guarantee the agricultural productivity and thereby to be able to feed the world’s rapidly growing population, irrigation has become very important. In parallel, the limited water resources lead to an increase in usage of poorly characterized sources of water, which is directly linked to a higher prevalence of foodborne diseases. Therefore, analyzing the microorganisms or even the complete microbiome of irrigation water used for food production can prevent the growing numbers of such cases. In this study, we compared the efficacy of MALDI-TOF Mass spectrometry (MALDI TOF MS) identification to 16S rRNA gene Sanger sequencing of waterborne microorganisms. Furthermore, we analyzed the whole microbial community of irrigation water using high-throughput 16S rRNA gene amplicon sequencing. The identification results of MALDI-TOF MS and 16S rRNA gene Sanger sequencing were almost identical at species level (66.7%; 64.3%). Based on the applied cultivation techniques, Acinetobacter spp., Enterobacter spp., Pseudomonas spp., and Brevundimonas spp. were the most abundant cultivable genera. In addition, the uncultivable part of the microbiome was dominated by Proteobacteria followed by Actinobacteria, Bacteroidota, Patescibacteria, and Verrucomicrobiota. Our findings indicate that MALDI-TOF MS offers a fast, reliable identification method and can act as an alternative to 16S rRNA gene Sanger sequencing of isolates. Moreover, the results suggest that MALDI-TOF MS paired with 16S rRNA gene amplicon sequencing have the potential to support the routine monitoring of the microbiological quality of irrigation water.
Measuring the Diameter of Single-Wall Carbon Nanotubes Using AFM
Authors: Vobornik Dusan , Chen Maohui , Zou Shan , Lopinski Gregory P.
Published: January 2023

In this work, we identify two issues that can significantly affect the accuracy of AFM measurements of the diameter of single-wall carbon nanotubes (SWCNTs) and propose a protocol that reduces errors associated with these issues. Measurements of the nanotube height under different applied forces demonstrate that even moderate forces significantly compress several different types of SWCNTs, leading to errors in measured diameters that must be minimized and/or corrected. Substrate and nanotube roughness also make major contributions to the uncertainty associated with the extraction of diameters from measured images. An analysis method has been developed that reduces the uncertainties associated with this extraction to <0.1 nm. This method is then applied to measure the diameter distribution of individual highly semiconducting enriched nanotubes in networks prepared from polyfluorene/SWCNT dispersions. Good agreement is obtained between diameter distributions for the same sample measured with two different commercial AFM instruments, indicating the reproducibility of the method. The reduced uncertainty in diameter measurements based on this method facilitates: (1) determination of the thickness of the polymer layer wrapping the nanotubes and (2) measurement of nanotube compression at tube–tube junctions within the network.
Infection of the Ex Vivo Tonsil Model by HTLV-1 Envelope-Pseudotyped Viruses
Authors: Langlois Mélanie , Bounou Salim , Tremblay Michel J. , Barbeau Benoit
Published: January 2023

Human T-cell leukemia virus type 1 (HTLV-1) is the causal agent of adult T-cell leukemia/lymphoma and HTLV-1-associated myelopathy/tropical spastic paraparesis. Its tropism is known to be broad in cultured cell lines, while in vivo data support a more selective transmission toward CD4+ T cells and the limited targeting of other hematopoietic cell types. An essential condition for HTLV-1 infection is cell-to-cell contact, to which both virological synapse and viral biofilm have been suggested to strongly contribute. As cell lines and animal models each present their own limitations in studying HTLV-1 replication, we have explored the use of an ex vivo model based on the secondary lymphoid tonsillar tissue. HIV-1 luciferase-expressing pseudotyped viruses bearing the HTLV-1 envelope protein at their surface were first shown to recapitulate the wide spectrum of infectivity of HTLV-1 toward various cell lines. Tonsil fragments were next exposed to pseudotyped viruses and shown to be reproducibly infected. Infection by HTLV-1 Env-pseudotyped viruses was blocked by different anti-gp46 antibodies, unlike infection by HIV-1 virions. The dose-dependent infection revealed a gradual increase in luciferase activity, which was again sensitive to anti-gp46 antibodies. Overall, these results suggest that the ex vivo tonsil model represents a reliable alternative for studying HTLV-1 replication and potentially viral latency, as well as early clonal formation.
Robustness Evaluation Process for Scheduling under Uncertainties
Authors: Himmiche Sara , Marangé Pascale , Aubry Alexis , Pétin Jean-François
Published: January 2023

Scheduling production is an important decision issue in the manufacturing domain. With the advent of the era of Industry 4.0, the basic generation of schedules becomes no longer sufficient to face the new constraints of flexibility and agility that characterize the new architecture of production systems. In this context, schedules must take into account an increasingly disrupted environment while maintaining a good performance level. This paper contributes to the identified field of smart manufacturing scheduling by proposing a complete process for assessing the robustness of schedule solutions: i.e., its ability to resist to uncertainties. This process focuses on helping the decision maker in choosing the best scheduling strategy to be implemented. It aims at considering the impact of uncertainties on the robustness performance of predictive schedules. Moreover, it is assumed that data upcoming from connected workshops are available, such that uncertainties can be identified and modelled by stochastic variables This process is supported by stochastic timed automata for modelling these uncertainties. The proposed approach is thus based on Stochastic Discrete Event Systems models and model checking techniques defining a highly reusable and modular process. The solution process is illustrated on an academic example and its performance (generecity and scalability) are deeply evaluated using statistical analysis. The proposed application of the evaluation process is based on the technological opportunities offered by the Industry 4.0.
Evaluating the Effectiveness of Modern Forecasting Models in Predicting Commodity Futures Prices in Volatile Economic Times
Authors: Vancsura László , Tatay Tibor , Bareith Tibor
Published: January 2023

The paper seeks to answer the question of how price forecasting can contribute to which techniques gives the most accurate results in the futures commodity market. A total of two families of models (decision trees, artificial intelligence) were used to produce estimates for 2018 and 2022 for 21- and 125-day periods. The main findings of the study are that in a calm economic environment, the estimation accuracy is higher (1.5% vs. 4%), and that the AI-based estimation methods provide the most accurate estimates for both time horizons. These models provide the most accurate forecasts over short and medium time periods. Incorporating these forecasts into the ERM can significantly help to hedge purchase prices. Artificial intelligence-based models are becoming increasingly widely available, and can achieve significantly better accuracy than other approximations.
Smartphones and Threshold-Based Monitoring Methods Effectively Detect Falls Remotely: A Systematic Review
Authors: Torres-Guzman Ricardo A. , Paulson Margaret R. , Avila Francisco R. , Maita Karla , Garcia John P. , Forte Antonio J. , Maniaci Michael J.
Published: January 2023

In the US, at least one fall occurs in at least 28.7% of community-dwelling seniors 65 and older each year. Falls had medical costs of USD 51 billion in 2015 and are projected to reach USD 100 billion by 2030. This review aims to discuss the extent of smartphone (SP) usage in fall detection and prevention across a range of care settings. A computerized search was conducted on six electronic databases to investigate the use of remote sensing technology, wireless technology, and other related MeSH terms for detecting and preventing falls. After applying inclusion and exclusion criteria, 44 studies were included. Most of the studies targeted detecting falls, two focused on detecting and preventing falls, and one only looked at preventing falls. Accelerometers were employed in all the experiments for the detection and/or prevention of falls. The most frequent course of action following a fall event was an alarm to the guardian. Numerous studies investigated in this research used accelerometer data analysis, machine learning, and data from previous falls to devise a boundary and increase detection accuracy. SP was found to have potential as a fall detection system but is not widely implemented. Technology-based applications are being developed to protect at-risk individuals from falls, with the objective of providing more effective and efficient interventions than traditional means. Successful healthcare technology implementation requires cooperation between engineers, clinicians, and administrators.
The Luminescence Hypothesis of Olfaction
Authors: Willeford Kenneth
Published: January 2023

A new hypothesis for the mechanism of olfaction is presented. It begins with an odorant molecule binding to an olfactory receptor. This is followed by the quantum biology event of inelastic electron tunneling as has been suggested with both the vibration and swipe card theories. It is novel in that it is not concerned with the possible effects of the tunneled electrons as has been discussed with the previous theories. Instead, the high energy state of the odorant molecule in the receptor following inelastic electron tunneling is considered. The hypothesis is that, as the high energy state decays, there is fluorescence luminescence with radiative emission of multiple photons. These photons pass through the supporting sustentacular cells and activate a set of olfactory neurons in near-simultaneous timing, which provides the temporal basis for the brain to interpret the required complex combinatorial coding as an odor. The Luminescence Hypothesis of Olfaction is the first to present the necessity of or mechanism for a 1:3 correspondence of odorant molecule to olfactory nerve activations. The mechanism provides for a consistent and reproducible time-based activation of sets of olfactory nerves correlated to an odor. The hypothesis has a biological precedent: an energy feasibility assessment is included, explaining the anosmia seen with COVID-19, and can be confirmed with existing laboratory techniques.
A Sustainable Approach to Delivering Programmable Peer-to-Peer Offline Payments
Authors: Mainetti Luca , Aprile Matteo , Mele Emanuele , Vergallo Roberto
Published: January 2023

Payment apps and digital wallets are powerful tools used to exchange e-money via the internet. However, with the progressive disappearance of cash, there is a need for the digital equivalent of physical banknotes to guarantee the same level of anonymity of private payments. Few efforts to solve the double-spending problem exist in P2P payments (i.e., in avoiding the possibility of a payer retaining copies of digital coins in absence of a trusted third party (TTP)), and further research efforts are needed to explore options to preserve the privacy of payments, as per the mandates of numerous central bank digital currency (CBDC) exploratory initiatives, such as the digital euro. Moreover, generic programmability requirements and energetic impacts should be considered. In this paper, we present a sustainable offline P2P payment scheme to face the double-spending problem by means of a one-time program (OTP) approach. The approach consists of wiping the business logic out of a client’s app and allowing financial intermediaries to inject a certified payment code into the user’s device, which will execute (asynchronously and offline) at the time of payment. To do so, we wrap each coin in a program at the time of withdrawal. Then the program exploits the trusted execution environment (TEE) of modern smartphones to transfer itself from the payer to the payee via a direct IoT link. To confirm the validity of the approach, we performed qualitative and quantitative evaluations, specifically focusing on the energetic sustainability of the proposed scheme. Results show that our payment scheme is energetically sustainable as the current absorbed for sending one coin is, at most, ~1.8 mAh on an Apple smartphone. We advance the state-of-the-art because the scheme meets the programmability, anonymity, and sustainability requirements (at the same time).
Deep-Q-Network-Based Packet Scheduling in an IoT Environment
Authors: Fu Xing , Kim Jeong Geun
Published: January 2023

With the advent of the Internet of Things (IoT) era, a wide array of wireless sensors supporting the IoT have proliferated. As key elements for enabling the IoT, wireless sensor nodes require minimal energy consumption and low device complexity. In particular, energy-efficient resource scheduling is critical in maintaining a network of wireless sensor nodes, since the energy-intensive processing of wireless sensor nodes and their interactions is too complicated to control. In this study, we present a practical deep Q-network (DQN)-based packet scheduling algorithm that coordinates the transmissions of multiple IoT devices. The scheduling algorithm dynamically adjusts the connection interval (CI) and the number of packets transmitted by each node within the interval. Through various experiments, we verify how effectively the proposed scheduler improves energy efficiency and handles the time-varying nature of the network environment. Moreover, we attempt to gain insight into the optimized packet scheduler by analyzing the policy of the DQN scheduler. The experimental results show that the proposed scheduling algorithm can further prolong a network’s lifetime in a dynamic network environment in comparison with that in other alternative schemes while ensuring the quality of service (QoS).
Evaluation of the Possible Protective Role of Nobiletin against Arsenic-Induced Liver Damage in Male Albino Rats
Authors: Ijaz Muhammad , Ahmed Aqsa , Al-Ghanim Khalid , Al-Misned Fahad , Riaz Mian , Kaimkhani Zahid , Mahboob Shahid
Published: January 2023

Arsenic (As) is a toxic contaminant present in organic and inorganic forms in the environment. Nobiletin (NOB) is a polymethoxy flavone that has recently gained substantial consideration due to its curative impacts. The present experiment was conducted to assess the hepatoprotective efficiency of NOB on As-generated hepatotoxicity. Twenty-four adult rats were equally distributed into four groups and designated as control, As (50 mg/kg)-treated, As + NOB (50 mg/kg and 25 mg/kg, respectively), and NOB (25 mg/kg)-treated groups. After 30 days, experimental animals were decapitated, then blood and tissue samples were collected for further analysis. The group treated with As showed a significant decrease in the activity of antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione (GSH), glutathione reductase (GSR), and total antioxidant status (TAS), and a substantial increase in the accumulation of As in liver tissues, levels of total oxidant status (TOS), hydrogen peroxide (H2O2), and lipid peroxidation (TBARS). Significant increases in alanine aminotransferase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST) levels were observed in As-treated rats. Moreover, nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, interleukin (IL)-6, and cyclo-oxygenase (COX)-2 activity, as well as the levels of pro-apoptotic markers (Bax, Caspase-3, and Caspase-9) were increased on exposure to As. In contrast, the anti-apoptotic marker (Bcl-2) level was significantly decreased. As administration showed a significant disturbance in hepatic tissue histology. However, cotreatment of NOB with As considerably increased the antioxidant enzyme activity, with a noteworthy reduction in the deposition of As in hepatic tissues, TBARS, and H2O2 levels. NOB-administrated rats showed considerable recovery in terms of inflammation, apoptosis, and histological damage. Hence, NOB can be considered a useful curative compound due to its medicinal properties against As-prompted hepatotoxicity.
Safety Evaluation of Curcumol by a Repeated Dose 28-Day Oral Exposure Toxicity Study in Rats
Authors: Yang Zhaoxu , Wang Sheng , Hong Yawen , Gai Renhua , Hong Wenxiang , Tang Bingbing , Lin Chunqin , Wang Xiaomeng , Wang Qiaojing , Chen Chao , Wang Jiajia , Weng Qinjie
Published: January 2023

Curcumol, a natural product isolated from the traditional Chinese medicine Rhizoma curcumae, possesses various potential therapeutic values in many diseases. However, evidence of its toxicological profile is currently lacking. In this study, a repeated toxicity study of curcumol was conducted for the first time. SD rats were exposed to doses of 250, 500, 1000 mg/kg in a selected dose formulation for 28 days through oral administration. The potential toxic effects of curcumol on the blood system were observed and further validated in vivo and in vitro. Moreover, other hematology and biochemistry parameters as well as the weight of organs were altered, but no related histopathological signs were observed, indicating these changes were not regarded as toxicologically relevant. Our current findings provide a complete understanding of the safety profile of curcumol, which may contribute to its further study of investigational new drug application.
Biochar as Sustainable Alternative and Green Adsorbent for the Remediation of Noxious Pollutants: A Comprehensive Review
Authors: Jha Stuti , Gaur Rama , Shahabuddin Syed , Tyagi Inderjeet
Published: January 2023

The current water crisis necessitates the development of new materials for wastewater treatment. A variety of nanomaterials are continuously being investigated for their potential as adsorbents for environmental remediation. Researchers intend to develop a low-cost, simple, and sustainable material that can cater to removal of pollutants. Biochar derived from biowaste is a potential candidate for the existing problem of water pollution. The review focuses on the various aspects of biochar, such as its sources, preparation methods, mechanism, applications for wastewater treatment, and its regeneration. Compared with other adsorbents, biochar is considered as an environmentally friendly, sustainable, and cost-effective substitute for waste management, climate protection, soil improvement, wastewater treatment, etc. The special properties of biochar such as porosity, surface area, surface charge, and functional groups can be easily modified by various chemical methods, resulting in improved adsorption properties. Therefore, in view of the increasing environmental pollution and the problems encountered by researchers in treating pollutants, biochar is of great importance. This review also highlights the challenges and prospective areas that can be explored and studied in more detail in the future.
Research on Control Method of Dual-Motor Load Simulator
Authors: Liu Xiaolin , Li Jinkai
Published: January 2023

Aiming at the output torque error of a steering gear electric load simulator caused by excess torque and backlash interference, an electric load simulator based on double-motor loading is designed. The double-motor loading mode is adopted in the structure, the mathematical model is established, and the sources of excess torque and backlash interference are analyzed. In the control strategy, firstly, a torque controller is designed as a feedback controller based on the improved error symbol robust integral control method, and then a backlash interference compensator is designed as a feedforward controller based on the drive redundancy strategy. Finally, a dual motor speed synchronization controller is designed based on the improved cross coupling control method to ensure the stable operation of the torque controller and backlash compensator in the dual-motor system. The simulation results show that the compound control method can reduce the tracking error to 1.13%, 4.44% less than the PID control method. The tracking error is only 1.54% in the case of redundant torque, backlash, and different parameters of dual motors. The method proposed in this paper can still output loading torque with high accuracy.