bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Experimental Setup and Methodology for the Investigation of Corrosion Fatigue in Metallic Biodegradable Implant Materials
Authors: Schumacher Lukas , Cetin Ikra-Nur , Bielefeldt Sira , Rupp Frank , Roehler Ariadne
Published: October 2024

Biodegradable implants as bone fixations may present a safe alternative to traditional permanent implants, reducing the risk of infections, promoting bone healing, and eliminating the need for removal surgeries. Structural integrity is an important consideration when choosing an implant material. As a biodegradable implant is being resorbed, until the natural bone has regrown, the implant material needs to provide mechanical stability. However, the corrosive environment of the human body may affect the fatigue life of the material. Conversely, mechanical stress can have an effect on electrochemical corrosion processes. This is known as corrosion fatigue. In the presented work, an experimental setup and methodology was established to analyze the corrosion fatigue of experimental bioresorbable materials while simultaneously monitoring the electrochemical processes. A double-walled measurement cell was constructed for a three-point bending test in Dulbecco‘s Phosphate-Buffered Saline (DPBS− −), which was used as simulated body fluid (SBF), at 37 ± 1 °C. The setup was combined with a three-electrode setup for corrosion measurements. Rod-shaped zinc samples were used to validate the setup’s functionality. Preliminary static and dynamic bending tests were carried out as per the outlined methodology to determine the test parameters. Open-circuit as well as potentiostatic polarization measurements were performed with and without mechanical loading. For the control, fatigue tests were performed in an air environment. The tested zinc samples were inspected via scanning electron microscopy (SEM). Based on the measured mechanical and electrochemical values as well as the SEM images, the effects of the different environments were investigated, and the setup’s functionality was verified. An analysis of the data showed that a comprehensive investigation of corrosion fatigue characteristics is feasible with the outlined approach. Therefore, this novel methodology shows great potential for furthering our understanding of the effects of corrosion on the fatigue of biodegradable implant materials.
Impact of Speed Sintering on Translucency, Opalescence and Microstructure of Dental Zirconia with a Combination of 5 mol% and 3 mol% Yttria-Stabilized Zirconia
Authors: Cho Mi-Hyang , Seol Hyo-Joung
Published: October 2024

Optical characteristics and microstructure of multilayered zirconia with different yttria contents in each layer can be influenced differently with a layer after speed sintering. The layer-wise translucency and opalescence of dental zirconia (E.max, E.max ZirCAD prime; Cercon, Cercon ht ML) after conventional (control) and speed sintering were analyzed using a spectrophotometer (n = 5). Specimens were subjected to microstructural analyses (n = 2) using field-emission scanning electron microscopy (FE-SEM) and phase analyses (n = 1) using high-resolution X-ray diffraction (HRXRD) and Rietveld refinement. The translucency parameter (TP) and opalescence parameter (OP) were analyzed using a 3-way ANOVA, followed by Scheffé’s post hoc test (α = 0.05). The average grain size was analyzed using the Welch’s t-test and Kruskal–Wallis test, followed by the Bonferroni–Dunn post hoc test (α = 0.05). Changes to the TP and OP after speed sintering were only observed in the dentin layers. Although the TP of E.max increased (p < 0.05), the difference was below the 50:50% perceptibility threshold (ΔE00 = 0.8). The OP of E.max decreased slightly, whereas that of Cercon increased slightly (p < 0.05). The microstructure and phase fraction of both zirconia barely changed. Therefore, speed sintering is considered to have a negligible clinical impact on the optical characteristics and microstructure.
Wireless PID-Based Control for a Single-Legged Rehabilitation Exoskeleton
Authors: Andersson Rabé , Cronhjort Mikael , Chilo José
Published: October 2024

The demand for remote rehabilitation is increasing, opening up convenient and effective home-based therapy for the sick and elderly. In this study, we use AnyBody simulations to analyze muscle activity and determine key parameters for designing a rehabilitation exoskeleton, as well as selecting the appropriate motor torque to assist patients during rehabilitation sessions. The exoskeleton was designed with a PID control mechanism for the precise management of motor positions and joint torques, and it operates in both automated and teleoperation modes. Hip and knee movements are monitored via smartphone-based IMU sensors, enabling real-time feedback. Bluetooth communication ensures seamless control during various training scenarios. Our study demonstrates that remotely controlled rehabilitation systems can be implemented effectively, offering vital support not only during global health crises such as pandemics but also in improving the accessibility of rehabilitation services in remote or underserved areas. This approach has the potential to transform the way physical therapy can be delivered, making it more accessible and adaptable to the needs of a larger patient population.
Neural Network-Based Design of a Buck Zero-Voltage-Switching Quasi-Resonant DC–DC Converter
Authors: Hinov Nikolay , Gilev Bogdan
Published: October 2024

In this paper, a design method using a neural network of a zero-voltage-switching buck quasi-resonant DC–DC converter is presented. The use of this innovative approach is justified because the design of quasi-resonant DC–DC converters is more complex compared to that of classical DC–DC converters. The converter is a piecewise linear system mathematically described by Kirchhoff’s laws and represented through switching functions. In this way, a mathematical model is used to generate data on the behavior of the state variables obtained under various design parameters. The obtained data are appropriately normalized, and a neural network is trained with them, which in practice serves as the inverse model of the device. An example is considered to demonstrate how this network can be used to design the converter. The key advantages of the proposed methodology include reducing the development time, improving energy efficiency, and the ability to automatically adapt to different loads and input conditions. This approach offers new opportunities for the design of advanced DC–DC converters in industries with high efficiency and performance requirements, such as the automotive industry and renewable energy sources.
Optimal Control of a Domestic Frost-Free Refrigerator Inlet Freezer from the Perspective of Reducing Energy Consumption
Authors: Sellila Nacer , Mouhali Waleed , Louaked Mohammed , Mechkour Houari
Published: October 2024

This study is dedicated to the development of a mathematical model based on shape optimal control of the inlet domestic frost-free refrigerator in the context of energy consumption reduction. A three-dimensional thermofluid model is established for the numerical studies. As the physical system (with shelves and fruits) can be seen as a porous medium, the coupled state equations of the transport mechanism (for velocity and temperature fields) are governed by Navier–Stokes–Forchheimer/Fourier equations. Then, based on the finite element method, the numerical scheme computation is made with FreeFem++ software (Version 4.6). Numerical results are shown for three different cases: empty without shelves, empty with shelves, and loaded with foods. For each case, the velocity and temperature fields’ results are discussed for the optimal configurations. The characterization of these physical parameters would help engineers in domestic frost-free refrigerator design.
OLG Model Analysis of Delayed Retirement and Social Pension Effects on Family-Based Elderly Care in China
Authors: Yu Wenguang , Gao Yixin , Wang Rui , Feng Xiaohan , Sun Rong , Huang Yujuan
Published: October 2024

Driven by the traditional concept of “Raising Children to Ensure Old-age Security”, China’s elderly care system has long relied on a family-centered approach, with intergenerational financial transfers (IFTs) serving as a major source of income for the elderly. However, as China’s population ages, the government’s plan to implement delayed retirement from 2025, along with pension reforms, is expected to reshape family-based eldercare and intergenerational financial dynamics. This paper employs an Overlapping Generation (OLG) model, grounded in unified growth theory, which incorporates a delayed retirement policy under the constraint of pension fund balance to examine the impact of adjusting the pension contribution rates (PCRs) and raising retirement age (RA) on IFT rates. The research findings indicate a negative correlation between RA, PCR, and IFT. Both policies have the potential to alleviate the burden on family-based eldercare. The integrated implementation of these two policies not only creates room for a phased reduction in pension contribution rates but also enables the effective utilization of senior workers’ expertise and experience.
Pediatric Microlaryngoscopy Experiences in a Tertiary Hospital: A Retrospective Analyses of 105 Procedures
Authors: Ocmen Elvan , Erdost Hale Aksu , Ersoy Sezin , Akdeniz Idris , Erdag Taner
Published: October 2024

Background and Objectives: Anesthesia for pediatric microlaryngoscopy/direct laryngoscopy and bronchoscopy (DLB) is very challenging. Airway management methods can vary from tubeless techniques to endotracheal intubation. In this study, we analyzed the pediatric DLB patients operated on in our tertiary hospital. Materials and Methods: After the ethics committee’s approval, we retrospectively searched the pediatric DLB patients operated on between 2018 and 2023. Demographic data, airway management, pathology, operation time, and complications were analyzed. Results: Fifty-seven pediatric patients and 105 procedures were analyzed. The most common pathology was subglottic stenosis (29.1%). More than half of the patients were younger than one year old (56.1%). The airway management was performed with intermittent mask ventilation (37.2%), endotracheal tube (33.3%), and tracheostomy cannula (29.5%). Intermittent mask ventilation was the airway management type in 66.0% of the infants. Conclusions: Here, we report our pediatric DLB experiences. Airway management is challenging and is dependent on the age and pathology of the child, and planned surgery. Excessive attention is required during airway surgeries such as DLB.
Influence of Nitride Coatings on Corrosion Resistance and the Biocompatibility of Titanium Alloy Products
Authors: Sotova Catherine , Yanushevich Oleg , Krikheli Natella , Kramar Olga , Vereschaka Alexey , Shehtman Semen , Milovich Filipp , Zhylinski Valery , Seleznev Anton , Peretyagin Pavel
Published: October 2024

The bioadhesion of bacteria to the surface of samples with Ti–TiN, Zr–ZrN, Zr–(Zr, Nb)N, and Zr–(Zr, Hf)N coatings was studied via incubation with gram-positive strains of Staphylococcus aureus. The samples were kept at 25 °C for 30 days in a 3% NaCl solution. The deposition of coatings slows, whereas oxidation processes intensify. The oxygen content on the TiN and (Zr, Nb)N coating surfaces was higher than that of the Ti sample without a coating. Samples with ZrN and, especially, (Zr, Hf)N coatings resist oxidation better. Regarding bioactivity toward S. aureus, the highest density of biological forms was observed on the surfaces of TiN and (Zr, Hf)N coatings. The lowest density was on the surfaces of uncoated, ZrN-coated, and (Zr, Nb)N-coated samples. On Ti–TiN, Zr–ZrN, and Zr–(Zr, Nb)N coatings, the formation of surface biostructures of a filamentary type was observed. In the uncoated sample, the biostructures have an island character, and in the sample with a Zr–(Zr, Hf)N coating, the formation of extensive areas of biostructures was observed. Between the biostructures and coating, a layer 5 to 15 nm thick was observed, presumably associated with bacterial adhesion. The presence of biostructures on the coating surface can activate or slow oxidation processes.
Effects of Stretch-Bending Straightening on the Tensile Properties of Cold Rolled Packaging Steel
Authors: Möller Sebastian , Nouskalis Dimitrios , Ehmke Björn , Matusch Dirk , Dresbach Christian , Altenbach Holm
Published: October 2024

Achieving perfect flatness, tension-free surfaces, and exceptional resistance to spring back are important characteristics of packaging steel, setting the standard for high-quality material performance. To guarantee these crucial parameters, the implementation of the stretch-bending process at the final stage of the production route is indispensable. Besides improving the flatness properties, the induced plastic deformation results in an accompanying change in the mechanical properties. This investigation focuses on understanding this change in mechanical properties due to different stretch-bending straightening process parameters. A multivariate predictive model is created to calculate a process window for achieving the desired flatness and also mechanical properties in the production of packaging steel. This model is validated by experiments with a laboratory facility.
Correction: Wang et al. CeO2-Supported TiO2−Pt Nanorod Composites as Efficient Catalysts for CO Oxidation. Molecules 2023, 28, 1867
Authors: Wang Haiyang , Yao Ruijuan , Zhang Ruiyin , Ma Hao , Gao Jianjing , Liang Miaomiao , Zhao Yuzhen , Miao Zongcheng
Published: October 2024

Following publication, concerns were raised regarding the peer-review process related to the publication of this article [...]
Correction: Yu et al. Nitrogen Doped Porous Biochar/β-CD-MOFs Heterostructures: Bi-Functional Material for Highly Sensitive Electrochemical Detection and Removal of Acetaminophen. Molecules 2023, 28, 2437
Authors: Yu Qi , Zou Jin , Yu Chenxiao , Peng Guanwei , Fan Guorong , Wang Linyu , Chen Shangxing , Lu Limin , Wang Zongde
Published: October 2024

Following publication, concerns were raised regarding the peer-review process related to the publication of this article [...]
Stimuli-Responsive Vesicles and Hydrogels Formed by a Single-Tailed Dynamic Covalent Surfactant in Aqueous Solutions
Authors: Xu Chunlin , Sun Na , Li Huaixiu , Han Xingchen , Zhang Ailing , Sun Panpan
Published: October 2024

Controlling the hierarchical self-assembly of surfactants in aqueous solutions has drawn much attention due to their broad range of applications, from targeted drug release, preparation of smart material, to biocatalysis. However, the synthetic procedures for surfactants with stimuli-responsive hydrophobic chains are complicated, which restricts the development of surfactants. Herein, a novel single-tailed responsive surfactant, 1-methyl-3-(2-(4-((tetradecylimino) methyl) phenoxy) ethyl)-3-imidazolium bromides (C14PMimBr), was facilely fabricated in situ by simply mixing an aldehyde-functionalized imidazolium cation (3-(2-(4-formylphenoxy) ethyl)-1-methyl imidazolium bromide, BAMimBr) and aliphatic amine (tetradecylamine, TDA) through dynamic imine bonding. With increasing concentration, micelles, vesicles, and hydrogels were spontaneously formed by the hierarchical self-assembly of C14PMimBr in aqueous solutions without any additives. The morphologies of vesicles and hydrogels were characterized by cryogenic transmission electron microscopy and scanning electron microscopy. The mechanical properties and microstructure information of hydrogels were demonstrated by rheological measurement, X-ray diffraction, and density functional theory calculation. In addition, the vesicles could be disassembled and reassembled with the breakage and reformation of imine bonds by adding acid/bubbling CO2 and adding alkali. This work provides a simple method for constructing stimuli-responsive surfactant systems and shows great potential application in targeted drug release, drug delivery, and intelligent materials.
Two Co(II) Isostructural Bifunctional MOFs via Mixed-Ligand Strategy: Syntheses, Crystal Structure, Photocatalytic Degradation of Dyes, and Electrocatalytic Water Oxidation
Authors: Yue Siyu , Tuo Mengqi , Sheng Yemeng , Guo Xinyu , Lu Jiufu , Wang Dong
Published: October 2024

The solvothermal reactions involving cobalt ions with 5-methylisophthalic acid (H2MIP) and 1,3-bis(2-methylimidazol)propane (BMIP) yielded two cobalt(II) organic frameworks: {[Co4(MIP)4(BMIP)3]·1/2DMA}n (SNUT-31) and {[Co4(MIP)4(BMIP)3]·(EtOH)2·H2O]}n (SNUT-32) where DMA represents N,N-dimethylacetamide and EtOH signifies ethyl alcohol. Single-crystal X-ray diffraction analyses reveal that SNUT-31 and SNUT-32 possess an isomorphic structure, featuring a unique 2-fold interpenetration of 3D frameworks in a parallel manner. Notably, both SNUT-31 and SNUT-32 demonstrate remarkable performance in electrocatalytic oxygen evolution reactions and exhibit exceptional photocatalytic degradation capabilities against a model comprising three distinct dyes: rhodamine B, methyl orange, and methyl blue.
Geographical Origin Traceability of Atractylodis Macrocephalae Rhizoma Based on Chemical Composition, Chromaticity, and Electronic Nose
Authors: Yang Ruiqi , Wang Yushi , Wang Jiayu , Guo Xingyu , Zhao Yuanyu , Zhu Keyao , Zhu Xintian , Zou Huiqin , Yan Yonghong
Published: October 2024

Atractylodis Macrocephalae Rhizoma (AMR) is a traditional Chinese medicine used for gastrointestinal diseases. With increased demand, there are more and more places of cultivation for AMR. However, the quality of AMR varies from place to place, and there is no good way to distinguish AMR from different origins at present. In this paper, we determined the content of eight chemical components including 60% ethanol extracts, essential oil, polysaccharides, atractylenolides, and atractylone, obtained the color parameters of AMR powder by colorimetry, and odor information was captured by the electronic nose, all of which were combined with machine learning to establish a rapid origin traceability method. The results of the principal component analysis of the chemical components revealed that Zhejiang AMR has a high comprehensive score and overall better quality. The Kruskal–Wallis test demonstrated that there are varying degrees of differences in chemical composition and color parameters across the different origin. However, the accuracy of the classification model is low (less than 80%), making it difficult to distinguish between different origins of AMR. The electronic nose demonstrated excellent classification performance in the traceability of AMR from different origins, with accuracy reaching more than 90% (PLS-DA: 96.88%, BPNN: 96.88%, PSO-SVM: 100%). Overall, this study clarified the quality differences of AMR among different origins, and a rapid and precise method combining machine learning was developed to trace the origin of AMR.
Optimization of the Process of Extracting Polysaccharides from Agrocybe aegerita and In Vitro Antioxidant and Anti-Aging Tests
Authors: Wang Yuhan , Yang Jingyi , Li Xiang , Yang Jingshuo , Wang Honglei
Published: October 2024

The extraction process of crude polysaccharides from Agrocybe aegerita was optimized, and the antioxidant and anti-aging effects of the crude polysaccharides were evaluated. The optimal extraction parameters for the polysaccharide were identified using the response surface methodology. The DPPH, hydroxy radical, and superoxide anion radical scavenging capacities were evaluated to determine the antioxidant properties of the AAPs. The effects of the AAPs on the lifespan, head-swing frequency, swallowing frequency, body-bending frequency, and stress resistance of Caenorhabditis elegans were determined. The optimal extraction conditions included a solid-to-liquid ratio that resulted in an extraction solution concentration of 0.034 g/mL, an extraction temperature of 92.64 °C, and an extraction time of 2.82 h. Under these conditions, the yield of the AAPs was 11.325% ± 0.996%. The IC50 of the AAPs for superoxide anion radical scavenging was 4.693 mg/mL. The AAPs reached their maximum activity at concentrations of about 2 mg/mL for DPPH and 5 mg/mL for the hydroxyl radical. The AAPs could prolong the lifespan and improve locomotion and the stress resistance of C. elegans. Our findings illustrate the potential of AAPs as an anti-aging and antioxidant agent, highlighting the use of this natural compound in the fields of food and pharmacology.
Layered Double Hydroxides as Systems for Capturing Small-Molecule Air Pollutants: A Density Functional Theory Study
Authors: Mohebbi Elaheh , Minnelli Cristina , Pavoni Eleonora , Sisti Laura , Laudadio Emiliano , Stipa Pierluigi
Published: October 2024

Air pollutants are usually formed by easily spreading small molecules, representing a severe problem for human health, especially in urban centers. Despite the efforts to stem their diffusion, many diseases are still associated with exposure to these molecules. The present study focuses on modeling and designing two-dimensional systems called Layered Double Hydroxides (LDHs), which can potentially trap these molecules. For this purpose, a Density Functional Theory (DFT) approach has been used to study the role of the elemental composition of LDHs, the type of counterion, and the ability of these systems to intercalate NO2 and SO2 between the LDH layers. The results demonstrated how the counterion determines the different possible spacing between the layers, modulating the internalization capacity of pollutants and determining the stability degree of the system for a long-lasting effect. The variations in structural properties, the density of states (DOS), and the description of the charge transfer have been reported, thus allowing the investigation of aspects that are difficult to observe from an experimental point of view and, at the same time, providing essential details for the effective development of systems that can counteract the spread of air pollutants.
The Adaptative Modulation of the Phosphinito–Phosphinous Acid Ligand: Computational Illustration Through Palladium-Catalyzed Alcohol Oxidation
Authors: Membrat Romain , Kondo Tété Etonam , Agostini Alexis , Vasseur Alexandre , Nava Paola , Giordano Laurent , Martinez Alexandre , Nuel Didier , Humbel Stéphane
Published: October 2024

The phosphinito–phosphinous acid ligand (PAP) is a singular bidentate-like self-assembled ligand exhibiting dissymmetric but interchangeable electronic properties. This unusual structure has been used for the generation of active palladium hydride through alcohol oxidation. In this paper, we report the first theoretical highlight of the adaptative modulation ability of this ligand within a direct H-abstraction path for Pd and Pt catalyzed alcohol oxidation. A reaction forces study revealed rearrangements in the ligand self-assembling system triggered by a simple proton shift to promote the metal hydride generation via concerted six-center mechanism. We unveil here the peculiar behavior of the phosphinito–phosphinous acid ligand in this catalysis.
Machine Learning as a “Catalyst” for Advancements in Carbon Nanotube Research
Authors: Chen Guohai , Tang Dai-Ming
Published: October 2024

The synthesis, characterization, and application of carbon nanotubes (CNTs) have long posed significant challenges due to the inherent multiple complexity nature involved in their production, processing, and analysis. Recent advancements in machine learning (ML) have provided researchers with novel and powerful tools to address these challenges. This review explores the role of ML in the field of CNT research, focusing on how ML has enhanced CNT research by (1) revolutionizing CNT synthesis through the optimization of complex multivariable systems, enabling autonomous synthesis systems, and reducing reliance on conventional trial-and-error approaches; (2) improving the accuracy and efficiency of CNT characterizations; and (3) accelerating the development of CNT applications across several fields such as electronics, composites, and biomedical fields. This review concludes by offering perspectives on the future potential of integrating ML further into CNT research, highlighting its role in driving the field forward.
Redefining Infarction Size for Small-Vessel Occlusion in Acute Ischemic Stroke: A Retrospective Case–Control Study
Authors: Huang Yen-Chu , Weng Hsu-Huei , Lin Leng-Chieh , Lee Jiann-Der , Yang Jen-Tsung , Tsai Yuan-Hsiung , Chen Chao-Hui
Published: October 2024

Background/Objectives: Small-vessel occlusion, previously referred to as lacunar infarcts, accounts for approximately one-third of all ischemic strokes, using an axial diameter of less than 20 mm on diffusion-weighted imaging. However, this threshold may not adequately differentiate small-vessel occlusion from other pathologies, such as branch atheromatous disease (BAD) and embolism. This study aimed to assess the clinical significance and pathological implications of acute small subcortical infarctions (SSIs) based on infarct diameter. Methods: We conducted a retrospective case–control study using data from stroke patients recorded between 2016 and 2021 of the Stroke Registry in Chang Gung Healthcare System. Patients with acute SSIs in penetrating artery territories were included. Key variables such as patient demographics, stroke severity, and medical history were collected. Infarcts were categorized based on size, and the presence of early neurological deterioration (END) and favorable functional outcomes were assessed. Results: Among the 855 patients with acute SSIs, the median age was 70 years and the median National Institutes of Health Stroke Scale (NIHSS) score at arrival was four. END occurred in 97 patients (11.3%). Those who experienced END were significantly less likely to achieve a favorable functional outcome compared to those who did not (18.6% vs. 59.9%, p < 0.001). The incidence of END increased progressively with infarct sizes of 15 mm or larger, with the optimal threshold for predicting END identified as 15.5 mm and for BAD, it was 12.1 mm. A multiple logistic regression analysis revealed that motor tract involvement [adjusted odds ratio (aOR) 2.3; 95% confidence interval (CI) 1.1–4.7], an initial heart rate greater than 90 beats per minute (aOR 2.3; 95% CI 1.2–4.3), and a larger infarct size (15 mm to less than 20 mm vs. 10 mm to less than 15 mm; aOR 3.0; 95% CI 1.4–6.3) were significantly associated with END. Conclusions: Our findings suggest that setting the upper limit for small-vessel occlusion at 15 mm would be more effective in distinguishing it from BAD. However, these findings should be interpreted in the context of the retrospective design and study population. Further multi-center research utilizing high-resolution vessel wall imaging is necessary to refine this threshold and enhance diagnostic accuracy.
Bridging the Gap: Improving Acute Ischemic Stroke Outcomes with Intravenous Thrombolysis Prior to Mechanical Thrombectomy
Authors: Seetge Jessica , Cséke Balázs , Karádi Zsófia Nozomi , Bosnyák Edit , Szapáry László
Published: October 2024

Background/Objectives: Current guidelines recommend intravenous thrombolysis (IVT) followed by mechanical thrombectomy (MT) for patients with acute ischemic stroke (AIS) caused by large vessel occlusion (LVO). This combined approach, known as bridging therapy (BT), is believed to increase the likelihood of a favorable functional outcome when administered within 4.5 h of symptom onset. However, the benefits of BT over direct mechanical thrombectomy (d-MT) remain debated. This study aimed to compare the outcomes of AIS-LVO patients undergoing MT within 6 h of symptom onset, with and without prior IVT. Methods: Within the prospective Transzlációs Idegtudományi Nemzeti Laboratórium (TINL) STROKE-registry, AIS-LVO patients admitted to the Department of Neurology, University of Pécs between February 2023 and June 2024 were investigated. The primary endpoint was the proportion of patients reaching functional independence at 90 days, defined as a modified Rankin Scale (mRS) score of 0–2. Secondary endpoints included clinical improvement at 72 h (National Institute of Health Stroke Scale [NIHSS] score of ≤1 or a change from baseline [ΔNIHSS] of ≥4) and successful recanalization (modified Thrombolysis in Cerebral Infarction [mTICI] score ≥ 2). Safety outcomes were evaluated based on thrombus migration and intracranial hemorrhage (ICH). Results were compared using linear and logistic regression analyses adjusted for baseline variables. Results: Of 82 patients, 51 (62.2%) received BT, while 31 (37.8%) underwent d-MT. The BT group showed a significantly higher rate of functional independence (45.7% vs. 17.2%, p = 0.014) and a lower 90-day mortality rate (13.7% vs. 35.5%, p = 0.029). Multivariate analysis revealed that IVT was independently associated with favorable functional outcomes (p = 0.011) and reduced mortality (p = 0.021). No significant differences were observed in terms of clinical improvement at 72 h, successful recanalization, thrombus migration, or hemorrhagic transformation between the groups. Conclusions: This study supports current guidelines recommending BT for thrombectomy-eligible AIS-LVO patients, offering new insights into the ongoing clinical debate.
ZIP8 Is Upregulated in the Testis of Zip14-/- Mice
Authors: Vungutur Varalakshmi , McCabe Shannon M. , Zhao Ningning
Published: October 2024

Background/Objectives: Manganese is an essential nutrient involved in various biological processes, including reproductive health, yet the mechanisms regulating its homeostasis in the testis remain poorly understood. Methods and Results: In this study, we investigated the expression and regulation of key manganese transporters—ZIP8, ZIP14, and ZnT10—in mouse testes. Immunoblotting analyses revealed that ZIP8 is expressed in the testes, while ZIP14 and ZnT10 were undetectable. Using Zip14 knockout (Zip14-/-) mice, which exhibit systemic manganese overload, we discovered a significant increase in manganese levels in the testis, accompanied by an upregulation of ZIP8. Importantly, the levels of other essential metals, such as iron, zinc, and copper, remained unchanged. Conclusions: Our findings suggest that ZIP8 plays a critical role in manganese transport in the testis, and its increased expression may contribute to manganese accumulation in the absence of ZIP14. This study advances our understanding of manganese homeostasis in the testis and its potential impact on male reproductive health.
Iodine Bioavailability and Biochemical Effects of Brassica oleracea var. sabellica L. Biofortified with 8-Hydroxy-7-iodo-5-quinolinesulfonic Acid in Wistar Rats
Authors: Krzemińska Joanna , Piątkowska Ewa , Kopeć Aneta , Smoleń Sylwester , Leszczyńska Teresa , Koronowicz Aneta
Published: October 2024

Background: Iodine is one of the essential trace elements for human life. The main objective of the biofortification of plants with iodine is to obtain food with a higher content of this element compared to conventional food. Biofortification of plants with iodine can increase the intake of this trace element by different populations. In addition, it may reduce the risk of iodine deficiency diseases. Objectives: The aim of the study was to investigate the effect of kale biofortified with 8-hydroxy-7-iodo-5-quinolinesulfonic acid (8-OH-7-I-5QSA) on iodine bioavailability and biochemical effects in Wistar rats. Methods: Kale biofortified with (8-OH-7-I-5QSA) was tested for iodine levels in urine, feces, and selected tissues using the ICP-MS/MS technique. The feeding experiment was designed to investigate potential changes in selected thyroid-regulated biochemical parameters in blood serum of Wistar rats. Results: The dietary intake of Wistar rats fed kale biofortified with (8-OH-7-I-5QSA) from both the “Oldenbor F1” and “Redbor F1” cultivars for 8 weeks resulted in significantly (p ≤ 0.05) higher iodine concentrations in the urine and kidneys of rats, which proves iodine bioavailability. Rats’ diets with “Oldenbor F1” and “Redbor F1” kale non- and -biofortified with 8-OH-7-I-5QSA had a significantly (p ≤ 0.05) lower or a tendency for lower concentration of TSH, triglyceride, total and direct bilirubin, TBARs, uric acid, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations in serum. Dietary intake of “Oldenbor F1” and “Redbor F1” kale biofortified with 8-OH-7-I-5QSA significantly (p ≤ 0.05) increased the total antioxidant status (TAS). Conclusions: Our study confirms that kale biofortified with iodine in organic form iodoquinoline 8-OH-7-I-5QSA is bioavailable and well absorbed by the Wistar rat and has a positive effect on selected biochemical parameters. The results obtained in this study may be highly predictive for further studies in humans.
Link Between Metabolic Syndrome, Blood Lipid Markers, Dietary Lipids, and Survival in Women with Early-Stage Breast Cancer
Authors: Bobin-Dubigeon Christine , Campion Loic , Bossard Clémence , Rossignol Elsa , Frenel Jean-Sébastien , Campone Mario , Bard Jean-Marie
Published: October 2024

Background/Objectives: Nearly 10% of cancers could be prevented through dietary changes. In addition, breast cancer (BC) is the most common cancer in women worldwide. Inadequate diet may lead to several metabolic abnormalities, including metabolic syndrome (MS). The goal of our study is to evaluate the link between survival after BC and MS, as well as diet lipids and circulating lipids. Methods: This study was performed in an early-stage BC cohort (n = 73): MS, dietary lipids, and circulating biological parameters, including leucocyte expression in cholesterol carriers (ATP-binding cassette transporter ABCA1, ABCG1), were determined before any medication intervention. The data of each patient were analyzed using univariate logistic regression and are expressed by HR, 95%CI [5th–95th]. All these parameters were explored with survival parameters using Cox regression analyses. Results: Overall survival (OS) and invasive disease-free survival (iDFS) were significantly longer for the women without metabolic syndrome with HR 4.7 [1.11–19.92] and p = 0.036, and 3.58 [1.23–10.44] and p = 0.019, respectively. The expression of ABCG1 in peripheral leucocytes, an ATP-binding cassette transporter involved in cholesterol and phospholipid trafficking, is significantly associated with iDFS (1.38 [1.1–1.9], p = 0.0048). MS is associated with more pejorative survival parameters in early-stage breast cancer. Paraoxonase (or PON) activities differ according to PON gene polymorphism, but also diet. A link between PON activities and survival parameters was suggested and needs to be clarified. Conclusions: This study emphasizes the link between survival parameters of early-stage breast cancer, metabolic syndrome, and some parameters related to lipid metabolism.
Malnutrition Diagnosis and Food Consumption in Subacute Post-Stroke Patients During Rehabilitation
Authors: Siotto Mariacristina , Guerrini Alessandro , Cocco Carola , Germanotta Marco , Fasano Alessio , Cipollini Valeria , Cortellini Laura , Pavan Arianna , Insalaco Sabina , Antonacci Erika , Ruco Elisabetta , Mosca Rita , Graziosi Adriana , Spatola Piera , Malanga Maria Rosaria , Campana Biagio , Aprile Irene Giovanna
Published: October 2024

Background: Stroke survivors frequently encounter malnutrition, adversely impacting clinical outcomes. Nevertheless, malnutrition and food consumption in post-stroke patients have not been frequently assessed, and their correlation with rehabilitation outcomes remains inadequately explored. The objective of this observational study was to evaluate malnutrition at admission in these patients, assess food consumption during a six-week rehabilitation program, and analyze their correlation with rehabilitation outcomes. Methods: Subacute post-stroke patients were evaluated at admission (T0) and after a six-week rehabilitation treatment (T1). At T0, we assessed clinical and demographic characteristics, and we diagnosed malnutrition according to the Global Leadership Initiative on Malnutrition (GLIM) criteria. Weight, BMI, hematochemical parameters, and activities of daily living with the modified Barthel Index (mBI) were evaluated at both T0 and T1; recovery was registered as a change in the mBI (ΔmBI = mBIT1 − mBIT0). Patients’ food consumption was recorded through visual plate waste estimation of three meals a day, 5 days a week, for six weeks of hospitalization for rehabilitation. Results: A total of 109 patients completed the study (51 women, mean age 69 ± 11). According to the GLIM criteria, 105 of these patients were at risk of malnutrition, while 43 were malnourished, with 15 severely malnourished. Malnourished patients wasted more food, with respect to non-malnourished patients, as measured by visual plate waste of total meals (25 ± 17% vs. 15 ± 14%; p = 0.001) and reached a lower ΔmBI. A linear regression analysis found a significant correlation between the ΔmBI, the waste of a “second dish”, which contained mainly protein, and serum albumin at admission, even after controlling for age. Conclusions: Malnutrition assessed with the GLIM criteria at admission and food consumption are two important nutritional parameters to evaluate in post-stroke patients hospitalized for rehabilitation due to their association with recovery.
Impact of Sanitation on Rodent Pullulation and Plague Status in an Informal Settlement on the Outskirts of Mahajanga (Madagascar)
Authors: Rahelinirina Soanandrasana , Razafiarimanga Zara Nomentsoa , Rajerison Minoarisoa , Djedanem Medard , Handschumacher Pascal , Jambou Ronan
Published: October 2024

Plague is a zoonotic disease caused by Yersinia pestis, and it is endemic in Madagascar. The plague cycle involves wild and commensal rodents and their fleas; humans are an accidental host. Madagascar is the country where plague burden is the highest. Plague re-emerged in Mahajanga, the western coast of Madagascar, in the 1990s and infected populations in the popular and insalubrious zones. Sanitation is considered a primary barrier to infection by excluding pathogens from the environment and reservoirs. Poor housing and hygiene and proximity to rodents and fleas in everyday life are major and unchanged risk factors of plague. The aim of this study was to measure the impact of sanitation on Yersinia pestis bacteria in human and small mammal reservoirs and flea vectors. This study was conducted on 282 households within 14 neighborhoods. Two sessions of sampling were conducted in 2013 and 2016. Small mammals were trapped inside and around houses using live traps. Fleas, blood and spleen were sampled to detect Y. pestis infection and antibodies and determine the level of plague circulation before and after the installation of sanitation in order to assess the impact of sanitation improvement on inhabitant health. Two major types of housing can be described, i.e., formal and informal (traditional), scattered in all the suburbs. Among the small mammals captured, 48.5% were Suncus murinus, and 70% of houses were infested. After sanitation, only 30% of houses remained infested, and most of them were located around the market. Fleas were mostly Xenopsylla cheopis. Before and after intervention, the overall prevalence of fleas was the same (index 4.5) across the 14 suburbs. However, the number of houses with fleas drastically decreased, and the flea index increased significantly in rodent-infested houses. Rodent abundance also decreased from 17.4% to 6.1% before and after intervention, respectively. A serology study highlights that plague is still circulating in Mahajanga, suggesting that small mammals maintain enzootic plague transmission in the city.
A Comprehensive Multi-Omics Study of Serum Alterations in Red Deer Infected by the Liver Fluke Fascioloides magna
Authors: Kuleš Josipa , Bujanić Miljenko , Rubić Ivana , Šimonji Karol , Konjević Dean
Published: October 2024

Liver fluke infections are acknowledged as diseases with global prevalence and significant implications for both veterinary and public health. The large American liver fluke, Fascioloides magna, is a significant non-native parasite introduced to Europe, threatening the survival of local wildlife populations. The aim of this study was to analyze differences in the serum proteome and metabolome between F. magna-infected and control red deer. Serum samples from red deer were collected immediately following regular hunting operations, including 10 samples with confirmed F. magna infection and 10 samples from healthy red deer. A proteomics analysis of the serum samples was performed using a tandem mass tag (TMT)-based quantitative approach, and a metabolomics analysis of the serum was performed using an untargeted mass spectrometry-based metabolomics approach. A knowledge-driven approach was applied to integrate omics data. Our findings demonstrated that infection with liver fluke was associated with changes in amino acid metabolism, energy metabolism, lipid metabolism, inflammatory host response, and related biochemical pathways. This study offers a comprehensive overview of the serum proteome and metabolome in response to F. magna infection in red deer, unveiling new potential targets for future research. The identification of proteins, metabolites, and related biological pathways enhances our understanding of host–parasite interactions and may improve current tools for more effective liver fluke control.
MicroRNAs as Promising Therapeutic Agents Against Prostate Cancer Resistant to Castration—Where Are We Now?
Authors: Ferreira Mariana , Morais Mariana , Medeiros Rui , Teixeira Ana Luísa
Published: October 2024

MicroRNAs are a conserved class of small, tissue-specific, non-coding RNAs that regulate gene expression to preserve cellular homeostasis. Proper miRNA expression is crucial for physiological balance because it affects numerous genetic pathways, including cell cycle control, proliferation, and apoptosis, through gene expression targeting. Deregulated miRNA expression has been implicated in several cancer types, including prostate cancer (PC), acting as tumor suppressors or oncogenes. Despite the availability of promising therapies to control tumor growth and progression, effective diagnostic and therapeutic strategies for different types of cancer are still lacking. PC continues to be a significant health challenge, particularly its castration-resistant (CRPC) form, which presents major therapeutic obstacles because of its resistance to conventional androgen deprivation treatments. This review explores miRNAs’ critical roles in gene regulation and cancer biology, as well as various miRNA delivery systems, highlighting their potential and the challenges in effectively targeting cancer cells. It aims to provide a comprehensive overview of the status of miRNA research in the fight against CRPC, summarizing miRNA-based therapies’ successes and limitations. It also highlights the promise of miRNAs as therapeutic agents for CRPC, underlining the need for further research to overcome existing challenges and move these therapies toward clinical applications.
Vascular Graft Impregnation with a Fosfomycin/Oritavancin Combination to Prevent Early Infection
Authors: Cruz Inês , Di Bella Stefano , D’Oria Mario , Lagatolla Cristina , Martins M. Cristina L. , Monteiro Cláudia
Published: October 2024

Background/Objectives: Vascular graft infections (VGIs) represent a life-threatening complication, occurring in 0.2–6% of patients following aortic prosthetic placements. Historically, the primary focus for reducing VGIs has been on prevention. Currently, antimicrobial grafts are not available on the market. This study aimed to evaluate the efficacy of combining two antibiotics, fosfomycin and oritavancin, impregnated into the commercially available GelweaveTM vascular graft as a prophylactic alternative against the most commonly implicated bacteria responsible for VGI. Methods: The antimicrobial activity of fosfomycin and oritavancin was assessed using the broth microdilution method, and a synergistic effect was demonstrated using the checkerboard assay against Staphylococcus epidermidis, methicillin-resistant Staphylococcus aureus, and vancomycin-resistant Enterococcus faecium. The antibiotics were impregnated into the commercial vascular graft through immersion, and the antimicrobial efficacy of the fosfomycin/oritavancin-impregnated graft was assessed over a period of 7 days. Results: Eradication of all microorganisms tested was achieved using impregnation solutions with concentrations of 40 mg/mL of fosfomycin and 256 µg/mL of oritavancin. Conclusions: Impregnation with the combination of fosfomycin/oritavancin proved to be a promising approach to prevent VGIs. Vascular grafts with impregnated antibiotics are not yet available on the market, and this work represents an important step toward the development of a new class of antimicrobial vascular grafts.
Demythologising the Given: Schlick, Cornelius, and Adorno contra Husserl
Authors: Vrahimis Andreas
Published: October 2024

After the attempt at collaboration between the Frankfurt School and the Vienna Circle failed in the late 1930s, Adorno stood at the forefront of critical theory’s polemics against ‘positivism’. Given these later polemical exchanges, some of the tendencies common to both movements have remained overlooked. Among these is their opposition to the phenomenological tradition. This paper focusses on certain features common to Schlick’s and Adorno’s critical responses to Husserl. The Machians, including Adorno’s supervisor Hans Cornelius, were targeted by Husserl’s onslaught against psychologism in 1900. The young Schlick’s Machian background had motivated his contribution to the Psychologismusstreit, in the context of which he launched a series of objections against Husserl’s ‘independence theory of truth’. Adorno’s later doctoral dissertation under Cornelius was also motivated by the effort to defend his master against Husserl’s objections. Schlick’s criticisms intensified in later works, where Husserl’s epistemology of intuition is seen as yet another instance of the widespread confusion between knowledge and acquaintance. Schlick’s warnings against Husserl’s turn towards the irrationalist fashions of his day find their echo in Adorno’s ‘Metakritik’. Apart from their broad agreement in how they understand Husserl’s positioning within his context, Schlick and Adorno also develop a similar criticism of Husserl’s account of intuition as failing in its aspiration to discover unmediated givenness, which it confuses with mediated conceptual knowledge. Unfortunately, while Adorno explicitly acknowledges Schlick’s critique of Husserl, he also misconstrues it as a scientistic rejection of metaphysical nonsense, thus failing to acknowledge the proximity to his approach.
Effects of Microplastics, Fertilization and Pesticides on Alien and Native Plants
Authors: Shi Xiong , Yang Guilin , Zheng Yulong
Published: October 2024

Plastic mulches, fertilizers and pesticides have been extensively employed in agriculture to increase crop yields, though it has also led to the inadvertent accumulation of them over time. These accumulations have the potential to disrupt the soil ecological process and subsequently impact the plant community composition. Alien plants always benefit from environmental variability, thus whether the accumulation of fertilizer, plastic, and pesticide in soil promotes the dominance of alien plants in an invaded community. Here, five aliens and co-occurring natives were selected as study materials, and a full factorial experiment was conducted to answer this question. Our study found that microplastics promote the biomass production of native plants at higher nutrient availability while having marginal influence on growth of alien plants. Alien plants exhibited a lower root mass fraction (RMF) with increased nutrient availability and a higher specific leaf area (SLA) in response to the addition of nutrients and microplastics. Pesticide residues in the soil also significantly decreased the root mass fraction of three species, but there was no significant difference between the effects on alien and native species. Overall, our results revealed that alien species adjusted their functional traits more quickly, but native species gained more growth advantages in response to fertilization and microplastics.