bluuebunny/crossref_metadata_2025 huggingface dataset:
Medical Applications of Artificial Intelligence (Legal Aspects and Future Prospects)
Authors: Laptev Vasiliy Andreevich , Ershova Inna Vladimirovna , Feyzrakhmanova Daria Rinatovna
Published: December 2021

Background: Cutting-edge digital technologies are being actively introduced into healthcare. The recent successful efforts of artificial intelligence in diagnosing, predicting and studying diseases, as well as in surgical assisting demonstrate its high efficiency. The AI’s ability to promptly take decisions and learn independently has motivated large corporations to focus on its development and gradual introduction into everyday life. Legal aspects of medical activities are of particular importance, yet the legal regulation of AI’s performance in healthcare is still in its infancy. The state is to a considerable extent responsible for the formation of a legal regime that would meet the needs of modern society (digital society). Objective: This study aims to determine the possible modes of AI’s functioning, to identify the participants in medical-legal relations, to define the legal personality of AI and circumscribe the scope of its competencies. Of importance is the issue of determining the grounds for imposing legal liability on persons responsible for the performance of an AI system. Results: The present study identifies the prospects for a legal assessment of AI applications in medicine. The article reviews the sources of legal regulation of AI, including the unique sources of law sanctioned by the state. Particular focus is placed on medical-legal customs and medical practices. Conclusions: The presented analysis has allowed formulating the approaches to the legal regulation of AI in healthcare.
Restoration of Hip Biomechanics after a Hemiarthroplasty for a Femoral Neck Fracture—Who Does It Better?
Authors: Tanzer Dylan , Baradaran Aslan , Hart Adam , Tanzer Michael
Published: December 2021

Introduction: The restoration of the preoperative biomechanics of the hip, in particular leg length and femoral offset, are critical in restoring normal function and diminishing the risk of dislocation following hip arthroplasty. This study compares the consistency of arthroplasty and non-arthroplasty orthopedic surgeons in restoring the normal biomechanics of the hip when performing a hemiarthroplasty for the treatment of a femoral neck fracture. Methods: We retrospectively reviewed the preoperative and postoperative digital radiographs of 175 hips that had a modular hemiarthroplasty for the treatment of a displaced femoral neck fracture at a Level 1 academic hospital. Fifty-two hips were treated by one of the three fellowship-trained arthroplasty surgeons (Group A), and 123 were treated by one of the nine non-arthroplasty fellowship-trained orthopedic surgeons (Group B). Results: Patients in Group A were more likely to have their femoral offset restored to normal than patients in Group B, both with respect to under correcting the offset (p = 0.031) and overcorrecting the offset (p = 0.010). Overall, there was no difference in restoration of leg lengths between the two groups (p = 0.869). Conclusions: Following a hemiarthroplasty for a displaced femoral neck fracture, the normal biomechanics of the hip are more likely to be restored by an arthroplasty-trained surgeon than by a non-arthroplasty-trained surgeon. Identifying the inconsistency of non-arthroplasty surgeons and, to a lesser degree, arthroplasty surgeons in restoring hip biomechanics is important for sensitizing surgeons to rectify this in the future with appropriate templating and femoral implant selection.
Metal Content and Structure of Textiles in Textile Metal Threads in Croatia from 17th to 20th Century
Authors: Šimić Kristina , Soljačić Ivo , Mudronja Domagoj , Petrović Leš Tihana
Published: December 2021

Textile metal threads were used to decorate historical Croatian textiles. There are three basic types of metal threads usually used on historical textiles in Croatia. These are narrow stripes, wires, and combined metal textile yarn called “srma”, made of metal thread spirally wrapped around the nonmetal textile yarn. Textile yarns were made of silk, linen, wool, or cotton. Metal threads were primarily made of gold, silver, and copper, and different alloys of these metals or threads are layered in the structure. Analysis of metal threads with three different methods was made and the most adequate method for the analysis of metal threads from historical textiles was established. Metal thread analysis was performed with scanning electron microscopy with an energy-dispersive X-ray detector (SEM-EDX), which was determined to be the most suitable for the analysis of historical textiles if cross-section analysis of metal threads is also performed. Textile threads from combined metal textile threads were analysed with a light microscope. This information of the metal threads’ content and structure as well as the composition of textile thread can lead to an understanding of the technology of production threads and also temporal and spatial dating of textile objects which is helpful to conservators and restorers of valuable historical textiles.
Modeling Semiarid River–Aquifer Systems with Bayesian Networks and Artificial Neural Networks
Authors: Maldonado Ana D. , Morales María , Navarro Francisco , Sánchez-Martos Francisco , Aguilera Pedro A.
Published: December 2021

In semiarid areas, precipitations usually appear in the form of big and brief floods, which affect the aquifer through water infiltration, causing groundwater temperature changes. These changes may have an impact on the physical, chemical and biological processes of the aquifer and, thus, modeling the groundwater temperature variations associated with stormy precipitation episodes is essential, especially since this kind of precipitation is becoming increasingly frequent in semiarid regions. In this paper, we compare the predictive performance of two popular tools in statistics and machine learning, namely Bayesian networks (BNs) and artificial neural networks (ANNs), in modeling groundwater temperature variation associated with precipitation events. More specifically, we trained a total of 2145 ANNs with different node configurations, from one to five layers. On the other hand, we trained three different BNs using different structure learning algorithms. We conclude that, while both tools are equivalent in terms of accuracy for predicting groundwater temperature drops, the computational cost associated with the estimation of Bayesian networks is significantly lower, and the resulting BN models are more versatile and allow a more detailed analysis.
Low Molecular Weight, 4-O-Sulfation, and Sulfation at Meta-Fucose Positively Promote the Activities of Sea Cucumber Fucoidans on Improving Insulin Resistance in HFD-Fed Mice
Authors: Hu Shiwei , Chen Sichun , Zhu Hongli , Du Mengyu , Jiang Wei , Liu Yu , Gao Xiang , Su Laijin , Xu Yangli
Published: December 2021

Fucoidans from sea cucumber (SC-FUC) have been proven to alleviate insulin resistance in several species. However, there are few studies that clarify the relationship between their structure and bioactivity. The present study evaluated the influence of molecular weight (Mw), sulfation concentrations (Cs), and sulfation position on improving insulin resistance using SC-FUC. Results showed that fucoidans with lower Mw exerted stronger effects. Having a similar Mw, Acaudina molpadioides fucoidans (Am-FUC) with lower Cs and Holothuria tubulosa fucoidans with higher Cs showed similar activities. However, Isostichopus badionotus fucoidans (higher Cs) activity was superior to that of low-Mw Thelenota ananas fucoidans (Ta-LFUC, lower Cs). Eliminating the effects of Mw and Cs, the bioactivity of Am-FUC with sulfation at meta-fucose exceeded that of Ta-FUC with sulfation at ortho-position. Moreover, the effects of Pearsonothuria graeffei fucoidans with 4-O-sulfation were superior to those of Am-LFUC with 2-O-sulfation. These data indicate that low Mw, 4-O-sulfation, and sulfation at meta-fucose contributed considerably to insulin resistance alleviation by SC-FUC, which could accelerate the development of SC-FUC as a potential food supplement to alleviate insulin resistance.
Formulation of New Media from Dairy and Brewery Wastes for a Sustainable Production of DHA-Rich Oil by Aurantiochytrium mangrovei
Authors: Russo Giovanni L. , Langellotti Antonio L. , Verardo Vito , Martín-García Beatriz , Di Pierro Prospero , Sorrentino Angela , Baselice Marco , Oliviero Maria , Sacchi Raffaele , Masi Paolo
Published: December 2021

Mozzarella stretching water (MSW) is a dairy effluent generated from mozzarella cheese production that does not have a real use and is destined to disposal, causing environmental problems and representing a high disposal cost for dairy producers. Spent brewery yeast (SBY) is another promising food waste produced after brewery manufacturing that could be recycled in new biotechnological processes. Aurantiochytrium mangrovei is an aquatic protist known as producer of bioactive lipids such as omega 3 long chain polyunsaturated fatty acids (ω3 LC-PUFA), in particular docosahexaenoic acid (DHA). In this work MSW and SBY have been used to formulate new sustainable growth media for A. mangrovei cultivation and production of DHA in an attempt to valorize these effluents. MSW required an enzymatic hydrolysis to enhance the biomass production. The new media obtained from hydrolysed MSW was also optimized using response surface methodologies, obtaining 10.14 g L−1 of biomass in optimized medium, with a DHA content of 1.21 g L−1.
Compression Stockings Improve Cardiac Output and Cerebral Blood Flow during Tilt Testing in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) Patients: A Randomized Crossover Trial
Authors: van Campen C. , Rowe Peter , Visser Frans
Published: December 2021

Background and Objectives: Orthostatic intolerance (OI) is a clinical condition in which symptoms worsen upon assuming and maintaining upright posture and are ameliorated by recumbency. OI has a high prevalence in patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). Limited data are available to guide the treatment of OI in ME/CFS patients. We and others have previously described patient-reported subjective improvement in symptoms using compression stockings. We hypothesized that these subjective reports would be accompanied by objective hemodynamic improvements. Materials and Methods: We performed a randomized crossover trial in 16 ME/CFS patients. Each underwent two 15-min head-up tilt table tests, one with and one without wearing knee-high compression stockings that provided 20–25 mm Hg compression. The order of the tests was randomized. We measured heart rate and blood pressure as well as cardiac output and cerebral blood flow (CBF) using extracranial Doppler of the internal carotid and vertebral arteries. Results: There were no differences in supine measurements between the 2 baseline measurements. There were no differences in heart rate and blood pressure at either end-tilt testing period. Compared to the test with the stockings off, the mean percentage reduction in cardiac output during the test with compression stockings on was lower, 15 (4)% versus 27 (6)% (p < 0.0001), as was the mean percentage CBF reduction, 14 (4)% versus 25 (5)% (p < 0.0001). Conclusion: In ME/CFS patients with orthostatic intolerance symptoms, cardiac output and CBF are significantly reduced during a tilt test. These abnormalities were present without demonstrable heart rate and blood pressure changes and were ameliorated by the use of compression stockings.
The Use of Targeted Monoclonal Antibodies in the Treatment of ABPA—A Case Series
Authors: O’Reilly Aoife , Dunican Eleanor
Published: December 2021

Allergic bronchopulmonary aspergillosis (ABPA) is a pulmonary disorder occurring in response to Aspergillus fumigatus that can complicate the course of asthma and cystic fibrosis. Here we present a case of acute ABPA without central bronchiectasis, a case of chronic active ABPA with central bronchiectasis, and a case of severe relapsing ABPA with central bronchiectasis. All three were initially treated with corticosteroids and antifungal agents but had an incomplete response. These patients were then treated with anti-IgE therapy with omalizumab before being switched to the anti-IL5R agent benralizumab. They responded well to both agents. These case reports highlight the potential role of omalizumab and benralizumab in the treatment of ABPA, but further studies are required to evaluate the effectiveness of these medications. Longer follow-up periods and objective measurements of the impact of treatment are necessary.
Egg Yolk Oil as a Plasticizer for Polylactic Acid Films
Authors: Carpintero María , Marcet Ismael , Rendueles Manuel , Díaz Mario
Published: December 2021

Polylactic acid (PLA) is known to be one of the most extensively used biodegradable thermoplastic polyesters, with the potential to replace conventional petroleum-based packaging materials; however, the low flexibility of films prepared using PLA has limited the applications of this biopolymer. In this study, in order to improve the mechanical properties of PLA films and to provide them with antioxidant properties, egg yolk oil was used as a biobased plasticizer. For this purpose, PLA films with increasing concentrations of egg yolk oil were prepared and the effects of this oil on the light transmission, transparency, colour, water vapour permeability, solubility, antioxidant activity and mechanical properties of the films were characterized. In addition, electron microscopy of the structure of the transverse section of the films was also performed. Results showed that the formulations with higher concentrations of egg yolk oil increased the films’ elasticity, and their light barrier and antioxidant properties. Finally, in order to test the films as a packaging material for food applications, extra virgin olive oil and resveratrol, both photosensitive compounds, were packed and exposed to ambient light. Overall, the results show the potential of egg yolk oil as an environmentally friendly plasticizer that can improve the flexibility of PLA films and provide them with additional photoprotective properties.
Design, Simulation and Optimization of an Additive Laser-Based Manufacturing Process for Gearbox Housing with Reduced Weight Made from AlSi10Mg Alloy
Authors: Magerramova Liubov , Isakov Vladimir , Shcherbinina Liana , Gukasyan Suren , Petrov Mikhail , Povalyukhin Daniil , Volosevich Darya , Klimova-Korsmik Olga
Published: December 2021

The gas turbine engine’s (GTE) development aims for the increasing the efficiency, strength, reliability and safety of its components. To create competitive engines, housing parts and components with high functionality and reduced weight are needed. Especially difficult in the design and production are the gearboxes for aviation GTE. Traditional technologies based on precision casting or material forming operations have significant limitations due to the complexity of fulfilling multiple different requirements. Nowadays, one of the progressive production techniques is additive manufacturing. The article presents the results of computational and experimental studies that substantiate the applicability of laser additive technology to reduce the mass of body parts by up to 15% while ensuring their strength properties. The physical and mechanical characteristics of aluminum alloys acceptable for the manufacturing of housing parts were analyzed. The necessary characteristics of the powder alloy of the Al-Si system and the technological parameters of the L-PBF of the modified housing of the gear reducer are established. Using the finite element method (FEM) the L-PBF process was numerically simulated and the technological modes for synthesis of the AlSi10Mg alloy powder were optimized. With the help of a serial 3D printer ProX320DMP, the prototype of a gear housing was manufactured.
Lightweight Multi-Scale Asymmetric Attention Network for Image Super-Resolution
Authors: Zhang Min , Wang Huibin , Zhang Zhen , Chen Zhe , Shen Jie
Published: December 2021

Recently, with the development of convolutional neural networks, single-image super-resolution (SISR) has achieved better performance. However, the practical application of image super-resolution is limited by a large number of parameters and calculations. In this work, we present a lightweight multi-scale asymmetric attention network (MAAN), which consists of a coarse-grained feature block (CFB), fine-grained feature blocks (FFBs), and a reconstruction block (RB). MAAN adopts multiple paths to facilitate information flow and accomplish a better balance of performance and parameters. Specifically, the FFB applies a multi-scale attention residual block (MARB) to capture richer features by exploiting the pixel-to-pixel correlation feature. The asymmetric multi-weights attention blocks (AMABs) in MARB are designed to obtain the attention maps for improving SISR efficiency and readiness. Extensive experimental results show that our method has comparable performance with fewer parameters than the current advanced lightweight SISR.
Bacterial Succession during Vermicomposting of Silver Wattle (Acacia dealbata Link)
Authors: Rosado Daniela , Pérez-Losada Marcos , Aira Manuel , Domínguez Jorge
Published: December 2021

Vermicomposting is the process of organic waste degradation through interactions between earthworms and microbes. A variety of organic wastes can be vermicomposted, producing a nutrient-rich final product that can be used as a soil biofertilizer. Giving the prolific invasive nature of the Australian silver wattle Acacia dealbata Link in Europe, it is important to find alternatives for its sustainable use. However, optimization of vermicomposting needs further comprehension of the fundamental microbial processes. Here, we characterized bacterial succession during the vermicomposting of silver wattle during 56 days using the earthworm species Eisenia andrei. We observed significant differences in α- and β-diversity between fresh silver wattle (day 0) and days 14 and 28, while the bacterial community seemed more stable between days 28 and 56. Accordingly, during the first 28 days, a higher number of taxa experienced significant changes in relative abundance. A microbiome core composed of 10 amplicon sequence variants was identified during the vermicomposting of silver wattle (days 14 to 56). Finally, predicted functional profiles of genes involved in cellulose metabolism, nitrification, and salicylic acid also changed significantly during vermicomposting. This study, hence, provides detailed insights of the bacterial succession occurring during vermicomposting of the silver wattle and the characteristics of its final product as a sustainable plant biofertilizer.
Genetic and Comparative Genome Analysis of Exiguobacterium aurantiacum SW-20, a Petroleum-Degrading Bacteria with Salt Tolerance and Heavy Metal-Tolerance Isolated from Produced Water of Changqing Oilfield, China
Authors: Su Zhaoying , Wang Shaojing , Yang Shicheng , Yin Yujun , Cao Yunke , Li Guoqiang , Ma Ting
Published: December 2021

The genome of Exiguobacterium aurantiacum SW-20 (E. aurantiacum SW-20), a salt-tolerant microorganism with petroleum hydrocarbon-degrading ability isolated from the Changqing Oilfield, was sequenced and analyzed. Genomic data mining even comparative transcriptomics revealed that some genes existed in SW-20 might be related to the salt tolerance. Besides, genes related to petroleum hydrocarbon degradation discovered in genomic clusters were also found in the genome, indicating that these genes have a certain potential in the bioremediation of petroleum pollutants. Multiple natural product biosynthesis gene clusters were detected, which was critical for survival in the extreme conditions. Transcriptomic studies revealed that some genes were significantly up-regulated as salinity increased, implying that these genes might be related to the salt tolerance of SW-20 when living in a high salt environment. In our study, gene clusters including salt tolerance, heavy metal tolerance and alkane degradation were all compared. When the same functional gene clusters from different strains, it was discovered that the gene composition differed. Comparative genomics and in-depth analysis provided insights into the physiological features and adaptation strategies of E. aurantiacum SW-20 in the oilfield environment. Our research increased the understanding of niches adaption of SW-20 at genomic level.
Transcriptome Analysis Unveils the Effects of Proline on Gene Expression in the Yeast Komagataella phaffii
Authors: Rumyantsev Andrey , Sidorin Anton , Volkov Artemii , Al Shanaa Ousama , Sambuk Elena , Padkina Marina
Published: December 2021

Komagataella phaffii yeast is one of the most important biocompounds producing microorganisms in modern biotechnology. Optimization of media recipes and cultivation strategies is key to successful synthesis of recombinant proteins. The complex effects of proline on gene expression in the yeast K. phaffii was analyzed on the transcriptome level in this work. Our analysis revealed drastic changes in gene expression when K. phaffii was grown in proline-containing media in comparison to ammonium sulphate-containing media. Around 18.9% of all protein-encoding genes were differentially expressed in the experimental conditions. Proline is catabolized by K. phaffii even in the presence of other nitrogen, carbon and energy sources. This results in the repression of genes involved in the utilization of other element sources, namely methanol. We also found that the repression of AOX1 gene promoter with proline can be partially reversed by the deletion of the KpPUT4.2 gene.
Effect of Fluidized Bed Drying, Matrix Constituents and Structure on the Viability of Probiotic Lactobacillus paracasei ATCC 55544 during Storage at 4 °C, 25 °C and 37 °C
Authors: Poddar Devastotra , Palmer Jon , Das Shantanu , Gaare Manju , Nag Arup , Singh Harjinder
Published: December 2021

The stabilization of probiotics for application in non-refrigerated food products is a challenging task. In the present study, probiotic Lactobacillus paracasei (Lacticaseibacillus paracasei) ATCC 55544 cells were immobilized in a dairy matrix comprising of whole milk powder, skim milk powder, or milk protein isolate using fluidized bed drying technology. The samples were taken out at different drying stages, with an apparent water activity (aw) of aw 0.5, aw 0.4, and aw 0.3, respectively, and vacuum-packed to maintain the aw and stored at three different temperatures of 4 °C, 25 °C, and 37 °C. The study evaluated the impact of matrix constituents, milk fat, protein, and carbohydrate on the viability of encapsulated probiotic L . paracasei ATCC 55544 during storage for 1 month. The whole milk powder matrix provided superior protection to the bacteria. Confocal Laser Scanning Microscopy (CLSM) was used to investigate the structure of the immobilizing matrix and the location of the probiotic L. paracasei cells embedded within the matrix. The CLSM study revealed that the probiotic bacterial cells are mostly embedded as clusters beneath the top layer. We hypothesize that the biofilm-like structure, together with the protective whole milk powder matrix, helps to retain the superior viability of probiotic cells during storage at non-refrigerated storage conditions of 25 °C and 37 °C.
Characterization of a Chromium-Bearing Carbon Steel Electric Arc Furnace Slag after Magnetic Separation to Determine the Potential for Iron and Chromium Recovery
Authors: Bru Kathy , Seron Alain , Morillon Agnieszka , Algermissen David , Lerouge Catherine , Menad Nourredine
Published: December 2021

This study investigates the potential to recover iron and chromium from a chromium-bearing carbon steel Electric Arc Furnace (EAF) slag. This slag contains indeed about 30 wt.% Fe and 2.5 wt.% Cr. However, the minerals are intergrown at small scale (<100 µm) and iron and chromium are mostly contained in spinel phases which makes the separation challenging. Several methods including Mössbauer spectroscopy, X-ray Diffraction, Scanning Electron Microscopy (SEM) and electron microprobe analysis were used in order to fully characterize the products obtained after a low-intensity magnetic separation of this carbon steel EAF slag, with the objective to define a pre-treatment process allowing the recovery of iron-rich particles and of a chromium-upgraded fraction. The results show that even if the magnetic separation seems to be not efficient in a first approach for producing an iron-rich/chromium-poor fraction, this fraction can be obtained by adding an attrition step which means that some separation mechanisms still occurred during the magnetic separation. However, it was not possible to produce a chromium-rich fraction. The main bottleneck for reaching a good separation is most probably the very fine liberation size of the iron and chromium bearing minerals.
Mono- and Bimetallic Nanoparticles Stabilized by an Aromatic Polymeric Network for a Suzuki Cross-Coupling Reaction
Authors: Nikoshvili Linda Zh. , Shkerina Kristina N. , Bykov Alexey V. , Sidorov Alexander I. , Vasiliev Alexander L. , Sulman Mikhail G. , Kiwi-Minsker Lioubov
Published: December 2021

This work addresses the Suzuki cross-coupling between 4-bromoanisole (BrAn) and phenylboronic acid (PBA) in an environmentally benign ethanol–water solvent catalysed by mono- (Pd) and bimetallic (PdAu, PdCu, PdZn) nanoparticles (NPs) stabilised within hyper-cross-linked polystyrene (HPS) bearing tertiary amino groups. Small Pd NPs of about 2 nm in diameters were formed and stabilized by HPS independently in the presence of other metals. High catalytic activity and complete conversion of BrAn was attained at low Pd loading. Introduction of Zn to the catalyst composition resulted in the formation of Pd/Zn/ZnO NPs, which demonstrated nearly double activity as compared to Pd/HPS. Bimetallic core-shell PdAu/HPS samples were 3-fold more active as compared to Pd/HPS. Both Pd/HPS and PdAu/HPS samples revealed promising stability confirmed by catalyst recycling in repeated reaction runs.
Solvent-Induced Assembly of One-Patch Silica Nanoparticles into Robust Clusters, Wormlike Chains and Bilayers
Authors: Liu Bin , Ravaine Serge , Duguet Etienne
Published: December 2021

We report the synthesis and solvent-induced assembly of one-patch silica nanoparticles in the size range of 100–150 nm. They consisted, as a first approximation, of silica half-spheres of which the truncated face was itself concave and carried in its center a polymeric patch made of grafted polystyrene chains. The multistage synthesis led to 98% pure batches and allowed a fine control of the patch-to-particle size ratio from 0.69 to 1.54. The self-assembly was performed in equivolume mixtures of tetrahydrofuran and ethanol, making the polymeric patches sticky and ready to coalesce together. The assembly kinetics was monitored by collecting samples over time and analyzing statistically their TEM images. Small clusters, such as dimers, trimers, and tetramers, were formed initially and then evolved in part into micelles. Accordingly to previous simulation studies, more or less branched wormlike chains and planar bilayers were observed in the long term, when the patch-to-particle size ratio was high enough. We focused also on the experimental conditions that could allow preparing small clusters in a good morphology yield.
A Stretchable and Self-Healing Hybrid Nano-Generator for Human Motion Monitoring
Authors: Zhu Yongsheng , Sun Fengxin , Jia Changjun , Zhao Tianming , Mao Yupeng
Published: December 2021

Transparent stretchable wearable hybrid nano-generators present great opportunities in motion sensing, motion monitoring, and human-computer interaction. Herein, we report a piezoelectric-triboelectric sport sensor (PTSS) which is composed of TENG, PENG, and a flexible transparent stretchable self-healing hydrogel electrode. The piezoelectric effect and the triboelectric effect are coupled by a contact separation mode. According to this effect, the PTSS shows a wide monitoring range. It can be used to monitor human multi-dimensional motions such as bend, twist, and rotate motions, including the screw pull motion of table tennis and the 301C skill of diving. In addition, the flexible transparent stretchable self-healing hydrogel is used as the electrode, which can meet most of the motion and sensing requirements and presents the characteristics of high flexibility, high transparency, high stretchability, and self-healing behavior. The whole sensing system can transmit signals through Bluetooth devices. The flexible, transparent, and stretchable wearable hybrid nanogenerator can be used as a wearable motion monitoring sensor, which provides a new strategy for the sports field, motion monitoring, and human-computer interaction.
Insight into the Properties of Plasmonic Au/TiO2 Activated by O2/Ar Plasma
Authors: Deng Xiaoqing , Ding Yu , Wang Xiaobing , Jia Xiaojing , Zhang Shuo , Li Xiang
Published: December 2021

The performance of CO oxidation over plasmonic Au/TiO2 photocatalysts is largely determined by the electric discharge characteristics and physicochemical properties of discharge gas. To explore the activation mechanism of Au/TiO2, an O2 and Ar mixture gas as a discharge gas was employed to activate Au/TiO2. The photocatalytic activity in CO oxidation over activated Au/TiO2 was obtained, and the electric discharge characteristics, Au nanoparticle size, surface chemical state, optical property and CO chemisorption were thoroughly characterized. As the O2 content increases from 10% to 50%, the amplitude of the current pulses increases, but the number of pulses and the discharge power decrease. The photocatalytic activity of Au/TiO2 rises rapidly at first and then remains constant at 75% when the O2 content is above 50%. Compared with the discharge gas of 10% and 30% O2/Ar, the sample activated by 50% O2/Ar plasma possesses less metallic Au and more surface oxygen species and carbonate species by X-ray photoelectron spectroscopy, which is consistent with UV-vis diffuse reflectance spectra and CO chemisorption. The CO chemisorption capacities of the activated samples are the same at a long exposure time due to the approximate Au nanoparticle size observed by transmission electron microscopy. An increase in carbonate species generated from the oxygen species on the surface of TiO2 is discovered.
An Attempt to Assess the Impact of Pandemic Restrictions on the Lifestyle, Diet, and Body Mass Index of Children with Endocrine Diseases—Preliminary Results
Authors: Zachurzok Agnieszka , Wójcik Małgorzata , Gawlik Aneta , Starzyk Jerzy B. , Mazur Artur
Published: December 2021

Background: Home isolation during the coronavirus 2019 (COVID-19) pandemic lockdown strongly impacted everyday life, affecting, in particular, eating habits and everyday activity. The aim of this study was to analyze the impact of the pandemic on behaviors and subsequent changes in body mass index (BMI) in children from Southern Poland. Methods: The study included 206 participants (104 females and 102 males) with a complete analysis of 177 participants (96 females and 81 males) with a mean age of 12.8 ± 2.6 years admitted to three pediatric endocrinology clinics (Rzeszów, Kraków, and Katowice) due to simple obesity, type 1 diabetes mellitus, somatotropin pituitary deficiency on growth hormone replacement therapy, and other endocrine and metabolic disorders between June and September 2020. The study used a self-prepared questionnaire regarding eating habits, physical activity, screen time, and sleep before and during the lockdown. Anthropometric measurements were performed under clinical settings twice (before the pandemic in January–March 2020, and in June–September 2020). Results: During the lockdown, BMI z-scores increased over the whole group, especially in obese children (0.073 ± 0.18, p = 0.002). The number of children who declared low and high physical activity of more than 60 min per day declined from 41.2% and 18.6% to 31.1% and 6.2% (p = 0.03 and p < 0.001), respectively; sleep times over 8 h increased (46.9% vs. 60.4% p = 0.007); screen times over 5 h daily increased (14.7% to 46.9%, p < 0.001). Eating habits did not change significantly. Conclusions: Daily physical activity and sleep levels were affected by the pandemic leading to the increase of BMI, especially in obese patients with endocrine disorders. During the COVID-19 pandemic, forward-thinking strategies must be developed to prevent childhood obesity.
Cathepsin B-Overexpressed Tumor Cell Activatable Albumin-Binding Doxorubicin Prodrug for Cancer-Targeted Therapy
Authors: Cho Hanhee , Shim Man Kyu , Yang Suah , Song Sukyung , Moon Yujeong , Kim Jinseong , Byun Youngro , Ahn Cheol-Hee , Kim Kwangmeyung
Published: December 2021

Prodrugs are bioreversible medications that should undergo an enzymatic or chemical transformation in the tumor microenvironment to release active drugs, which improve cancer selectivity to reduce toxicities of anticancer drugs. However, such approaches have been challenged by poor therapeutic efficacy attributed to a short half-life and low tumor targeting. Herein, we propose cathepsin B-overexpressed tumor cell activatable albumin-binding doxorubicin prodrug, Al-ProD, that consists of a albumin-binding maleimide group, cathepsin B-cleavable peptide (FRRG), and doxorubicin. The Al-ProD binds to in situ albumin, and albumin-bound Al-ProD indicates high tumor accumulation with prolonged half-life, and selctively releases doxorubicin in cathepsin B-overexpressed tumor cells, inducing a potent antitumor efficacy. Concurrently, toxicity of Al-ProD toward normal tissues with innately low cathepsin B expression is significantly reduced by maintaining an inactive state, thereby increasing the safety of chemotherapy. This study offers a promising approach for effective and safe chemotherapy, which may open new avenues for drug design and translational medicine.
Profiling of Polyphenolic Compounds of Leontopodium alpinum Cass Callus Cultures Using UPLC/IM-HRMS and Screening of In Vitro Effects
Authors: Pralea Ioana-Ecaterina , Moldovan Radu-Cristian , Țigu Adrian-Bogdan , Petrache Alina-Maria , Hegheș Simona-Codruța , Mitoi Monica , Cogălniceanu Gina , Iuga Cristina-Adela
Published: December 2021

Leontopodium alpinum Cass. (edelweiss) is recognized as a frequent constituent of anti-aging skin care products, providing increased antioxidant and anti-inflammatory defense. Considering the growing demand and the protected status of edelweiss in many countries, alternative methods of production have been developed, one of them being callus culturing. This study reports the phytochemical composition of a methanolic extract of L. alpinum callus cultures, characterized by liquid chromatography coupled to ion-mobility high resolution mass spectrometry (UPLC/IM-HRMS). The methanolic extract exhibited strong free radical scavenging activity (122.19 ± 7.28 mg AAE/g dw), while the quantitative evaluation revealed that four major constituents (phenylpropanoid derivatives) represent 57.13% (m/m) of the extract. Consequently, a screening of antiproliferative effects was performed on ten cancer cell lines, representative of prostate, colon, lung and breast cancer, showing inhibition of colony formation in all cases. These results provide a comprehensive phytochemical characterization of L. alpinum callus cultures using advanced IM-HRMS, while the in vitro explorations confirmed the potent antioxidant properties of edelweiss which are worth exploring further in cancer prevention.
Metabolic Variation among Fruits of Different Chili Cultivars (Capsicum spp.) Using HPLC/MS
Authors: Zamljen Tilen , Medič Aljaž , Veberič Robert , Hudina Metka , Jakopič Jerneja , Slatnar Ana
Published: December 2021

Chilies are widely cultivated for their rich metabolic content, especially capsaicinoids. In our study, we determined individual sugars, organic acids, capsaicinoids, and total phenolic content in pericarp, placenta, and seeds of Capsicum annuum L., Capsicum chinense Jacq. and Capsicum baccatum L. by HPLC/MS. Dry weight varied in the cultivar ‘Cayenne’, with the first fruit having the lowest dry weight, with 4.14 g. The total sugar content and organic acid content did not vary among the fruits of all three cultivars. The cultivar ‘Cayenne’ showed differences in total phenolic and capsaicinoid content between fruits in the placenta, with the first fruit having the highest content of total phenolics (27.85 g GAE/kg DW) and total capsaicinoids (16.15 g/kg DW). Of the three cultivars studied, the cultivar ‘Habanero Orange’ showed the least variability among fruits in terms of metabolites. The content of dihydrocapsaicin, nordihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin in the seeds of the second fruit was higher than that of the first fruit of the cultivar ‘Bishop Crown’. The results of our study provided significant insight into the metabolomics of individual fruits of the same chili plant. We have thus increased our understanding of how certain metabolites are distributed between fruits at different levels of the same plant and different parts of the fruit. This could be further investigated when chilies are exposed to different environmental stresses.
Modulational Instability of Ion-Acoustic Waves in Pair-Ion Plasma
Authors: Jahan Sharmin , Shikha Rubaiya Khondoker , Mannan Abdul , Mamun A A
Published: December 2021

The modulational instability (MI) of ion-acoustic waves (IAWs) is examined theoretically in a four-component plasma system containing inertialess electrons featuring a non-thermal, non-extensive distribution, iso-thermal positrons, and positively as well as negatively charged inertial ions. In this connection, a non-linear Schrödinger equation (NLSE), which dominates the conditions for MI associated with IAWs, is obtained by using the reductive perturbation method. The numerical analysis of the NLSE reveals that the increment in non-thermality leads to a more unstable state, whereas the enhancement in non-extensivity introduces a less unstable state. It also signifies the bright (dark) ion-acoustic (IA) envelope solitons mode in the unstable (stable) domain. The conditions for MI and its growth rate in the unstable regime of the IAWs are vigorously modified by the different plasma parameters (viz., non-thermal, non-extensive q-distributed electron, iso-thermal positron, the ion charge state, the mass of the ion and positron, non-thermal parameter α, the temperature of electron and positron, etc.). Our findings may supplement and add to prior research in non-thermal, non-extensive electrons and iso-thermal positrons that can co-exist with positive as well as negative inertial ions.
Statistical Analysis of Synthesis Parameters to Fabricate PVDF/PVP/TiO2 Membranes via Phase-Inversion with Enhanced Filtration Performance and Photocatalytic Properties
Authors: Nascimben Santos Erika , Fazekas Ákos , Hodúr Cecilia , László Zsuzsanna , Beszédes Sándor , Scheres Firak Daniele , Gyulavári Tamás , Hernádi Klára , Arthanareeswaran Gangasalam , Veréb Gábor
Published: December 2021

Non-solvent induced phase-inversion is one of the most used methods to fabricate membranes. However, there are only a few studies supported by statistical analysis on how the different fabrication conditions affect the formation and performance of membranes. In this paper, a central composite design was employed to analyze how different fabrication conditions affect the pure water flux, pore size, and photocatalytic activity of polyvinylidene fluoride (PVDF) membranes. Polyvinylpyrrolidone (PVP) was used to form pores, and titanium dioxide (TiO2) to ensure the photocatalytic activity of the membranes. The studied bath temperatures (15 to 25 °C) and evaporation times (0 to 60 s) did not significantly affect the pore size and pure water flux of the membranes. The concentration of PVDF (12.5 to 17.5%) affected the viscosity, formation capability, and pore sizes. PVDF at high concentrations resulted in membranes with small pore sizes. PVP affected the pore size and should be used to a limited extent to avoid possible hole formation. TiO2 contents were responsible for the decolorization of a methyl orange solution (10−5 M) up to 90% over the period studied (30 h). A higher content of TiO2 did not increase the decolorization rate. Acidic conditions increased the photocatalytic activity of the TiO2-membranes.
Thermal Behaviour of Microgels Composed of Interpenetrating Polymer Networks of Poly(N-isopropylacrylamide) and Poly(acrylic acid): A Calorimetric Study
Authors: Franco Silvia , Buratti Elena , Nigro Valentina , Bertoldo Monica , Ruzicka Barbara , Angelini Roberta
Published: December 2021

Stimuli-responsive microgels have recently attracted great attention in fundamental research as their soft particles can be deformed and compressed at high packing fractions resulting in singular phase behaviours. Moreover, they are also well suited for a wide variety of applications such as drug delivery, tissue engineering, organ-on-chip devices, microlenses fabrication and cultural heritage. Here, thermoresponsive and pH-sensitive cross-linked microgels, composed of interpenetrating polymer networks of poly(N-isopropylacrylamide) (PNIPAM) and poly(acrylic acid) (PAAc), are synthesized by a precipitation polymerization method in water and investigated through differential scanning calorimetry in a temperature range across the volume phase transition temperature of PNIPAM microgels. The phase behaviour is studied as a function of heating/cooling rate, concentration, pH and PAAc content. At low concentrations and PAAc contents, the network interpenetration does not affect the transition temperature typical of PNIPAM microgel in agreement with previous studies; on the contrary, we show that it induces a marked decrease at higher concentrations. DSC analysis also reveals an increase of the overall calorimetric enthalpy with increasing concentration and a decrease with increasing PAAc content. These findings are discussed and explained as related to emerging aggregation processes that can be finely controlled by properly changing concentration, PAAc content an pH. A deep analysis of the thermodynamic parameters allows to draw a temperature–concentration state diagram in the investigated concentration range.
ABC-Type Triblock Copolyacrylamides via Copper-Mediated Reversible Deactivation Radical Polymerization
Authors: Alsubaie Fehaid , Alothman Othman , Fouad Hassan , Mourad Abdel-Hamid
Published: December 2021

The aqueous Cu(0)-mediated reversible deactivation radical polymerization (RDRP) of triblock copolymers with two block sequences at 0.0 °C is reported herein. Well-defined triblock copolymers initiated from PHEAA or PDMA, containing (A) 2-hydroxyethyl acrylamide (HEAA), (B) N-isopropylacrylamide (NIPAM) and (C) N, N-dimethylacrylamide (DMA), were synthesized. The ultrafast one-pot synthesis of sequence-controlled triblock copolymers via iterative sequential monomer addition after full conversion, without any purification steps throughout the monomer additions, was performed. The narrow dispersities of the triblock copolymers proved the high degree of end-group fidelity of the starting macroinitiator and the absence of any significant undesirable side reactions. Controlled chain length and extremely narrow molecular weight distributions (dispersity ~1.10) were achieved, and quantitative conversion was attained in as little as 52 min. The full disproportionation of CuBr in the presence of Me6TREN in water prior to both monomer and initiator addition was crucially exploited to produce a well-defined ABC-type triblock copolymer. In addition, the undesirable side reaction that could influence the living nature of the system was investigated. The ability to incorporate several functional monomers without affecting the living nature of the polymerization proves the versatility of this approach.
Sustainability Evaluation of Polyhydroxyalkanoate Production from Slaughterhouse Residues Utilising Emergy Accounting
Authors: Shahzad Khurram , Rehan Mohammad , Rashid Muhammad Imtiaz , Ali Nadeem , Summan Ahmed Saleh , Ismail Iqbal Muhammad Ibrahim
Published: December 2021

High raw material prices and rivalry from the food industry have hampered the adoption of renewable resource-based goods. It has necessitated the investigation of cost-cutting strategies such as locating low-cost raw material supplies and adopting cleaner manufacturing processes. Exploiting waste streams as substitute resources for the operations is one low-cost option. The present study evaluates the environmental burden of biopolymer (polyhydroxyalkanoate) production from slaughtering residues. The sustainability of the PHA production process will be assessed utilising the Emergy Accounting methodology. The effect of changing energy resources from business as usual (i.e., electricity mix from the grid and heat provision utilising natural gas) to different renewable energy resources is also evaluated. The emergy intensity for PHA production (seJ/g) shows a minor improvement ranging from 1.5% to 2% by changing only the electricity provision resources. This impact reaches up to 17% when electricity and heat provision resources are replaced with biomass resources. Similarly, the emergy intensity for PHA production using electricity EU27 mix, coal, hydropower, wind power, and biomass is about 5% to 7% lower than the emergy intensity of polyethylene high density (PE-HD). In comparison, its value is up to 21% lower for electricity and heat provision from biomass.
Features of the Gas-Permeable Crystalline Phase of Poly-2,6-dimethylphenylene Oxide
Authors: Alentiev Alexander Yu , Levin Ivan S. , Belov Nikolay A. , Nikiforov Roman Yu , Chirkov Sergey V. , Bezgin Denis A. , Ryzhikh Victoria E. , Kostina Julia V. , Shantarovich Victor P. , Grunin Leonid Yu
Published: December 2021

Poly-2,6-dimethylphenylene oxide (PPO) film samples with varying degrees of crystallinity (from 0 to 69%) were obtained by means of different techniques. The films were studied by various physicochemical methods (Fourier-transform infrared spectroscopy, positron annihilation lifetime spectroscopy, X-ray diffraction, and 1H nuclear magnetic resonance relaxation). Solubility coefficients of gases in the PPO samples were measured via sorption isotherms of gases by volumetric technique with chromatographic detection. The apparent activation energy of permeation and the activation energy of diffusion of all gases were estimated based on temperature dependences of gas permeability and diffusivity for amorphous and semi-crystalline PPO in the range of 20–50 °C. The peculiarities of free volume, density, and thermal properties of gas transport confirm the nanoporosity of the gas-permeable crystalline phase of PPO. So, the PPO can be included in the group of organic molecular sieves.