bluuebunny/crossref_metadata_2025 huggingface dataset:
Alzheimer’s Dementia: The Emerging Role of Positron Emission Tomography
Authors: Tripathi Shailendra Mohan , Murray Alison D.
Published: March 2021

Alzheimer’s disease (AD) is the most common cause of dementia and accounts for approximately 50% to 80% of all cases of dementia. The diagnosis of probable AD is based on clinical criteria and overlapping clinical features pose a challenge to accurate diagnosis. However, neuroimaging has been included as a biomarker in various published criteria for the diagnosis of probable AD, in the absence of a confirmatory diagnostic test during life. Advances in neuroimaging techniques and their inclusion in diagnostic and research criteria for the diagnosis of AD includes the use of positron emission tomography (PET) imaging as a biomarker in various therapeutic and prognostic studies in AD. The development and application of a range of PET tracers will allow more detailed assessment of people with AD and will improve diagnostic specificity and targeted therapy of AD. The aim of this review is to summarize current evidence on PET imaging using the non-specific tracer [18F]fluorodeoxyglucose and specific tracers that target amyloid and tau pathology in people with AD.
Plasmodium falciparum Malaria Parasites in Ghana Show Signatures of Balancing Selection at Artemisinin Resistance Predisposing Background Genes
Authors: Tandoh Kwesi Z , Amenga-Etego Lucas , Quashie Neils B , Awandare Gordon , Wilson Michael , Duah-Quashie Nancy O
Published: March 2021

Sub-Saharan Africa is courting the risk of artemisinin resistance (ARTr) emerging in Plasmodium falciparum malaria parasites. Current molecular surveillance efforts for ARTr have been built on the utility of P. falciparum kelch13 ( pfk13) validated molecular markers. However, whether these molecular markers will serve the purpose of early detection of artemisinin-resistant parasites in Ghana is hinged on a pfk13 dependent evolution. Here, we tested the hypothesis that the background pfk13 genome may be present before the pfk13 ARTr-conferring variant(s) is selected and that signatures of balancing selection on these genomic loci may serve as an early warning signal of ARTr. We analyzed 12 198 single nucleotide polymorphisms (SNPs) in Ghanaian clinical isolates in the Pf3K MalariaGEN dataset that passed a stringent filtering regimen. We identified signatures of balancing selection in 2 genes (phosphatidylinositol 4-kinase and chloroquine resistance transporter) previously reported as background loci for ARTr. These genes showed statistically significant and high positive values for Tajima’s D, Fu and Li’s F, and Fu and Li’s D. This indicates that the biodiversity required to establish a pfk13 background genome may have been primed in clinical isolates of P. falciparum from Ghana as of 2010. Despite the absence of ARTr in Ghana to date, our finding supports the current use of pfk13 for molecular surveillance of ARTr in Ghana and highlights the potential utility of monitoring malaria parasite populations for balancing selection in ARTr precursor background genes as early warning molecular signatures for the emergence of ARTr.
Vibration characteristic analyses of medium-and small-span girder bridge groups in highway systems based on machine learning models
Authors: Lu Guanya , Wang Kehai , Guo Weizuo
Published: March 2021

There are large amounts of small-and medium-span girder bridges which bear structural similarity, while the large-scale bridge structures are generally limited in the timely applications of structural vibration characteristics. Therefore, in this study a framework based on machine learning models was proposed to analyze the vibration characteristics of specific line bridge groups. The probability distributions of structural, geometric, and material properties of bridge groups in specific lines were obtained using statistical tools and a Latin hypercube sampling method was used to generate reasonable sample sets for the bridges group, and parameterized finite element models of the bridges were established. Then, the optimal models were tuned and determined to predict fundamental mode and period by the 10-fold cross-validation method applying the numerical simulation results. This study’s results showed that the random forest models divided the vibration modes of the bridge groups into the longitudinal vibrations of the main girders and the longitudinal vibrations of the adjacent spans and side piers with a classification accuracy of greater than 90%, while the artificial neural network models exhibited the lowest normalized mean square error for the periods. The periods mainly ranged between 0.7 and 1.5 s. Furthermore, the bearing settings, ratios of the pier height to section diameters, and boundary types were determined to be the most significant properties influencing the fundamental modes and periods of the examined bridges, by respectively observing the reduced value of the random forest Gini indices and distribution of the generalized weight value of the input variables in artificial neural networks. This study provides an intelligent and efficient method for obtaining vibration characteristics of bridges group for a specific network.
Adapting to the unpredictable: The story of a Norwegian-Russian study of NGOs in the Murmansk region during a pandemic
Authors: Sørly Rita , Kårtveit Bård , Nygaard Vigdis , Normann Anne Katrine , Ivanova Ludmila , Britvina Svetlana , Riabova Larissa
Published: March 2021

This essay aims to contribute to the understanding of the COVID-19 pandemic’s impact on a Norwegian- Russian social work research project. The reflexive act of writing about the project’s disrupted startup is an exercise of flexibility and dynamics in the research process. Well known grips for those of us working with qualitative inquiry. Still, the unpredictable landscape, uncertainty of what would come next, and the insecurity in the present moved us as researchers towards new local and contextual knowledge, forced by the pandemic’s extensive effects.
“Thanks for hearing me out”: Voices of social work students during COVID-19
Authors: Cole Sarah J , Mitra Samantha S , Robinson Jennifer A , Jen Sarah , Paceley Megan S , Carr Kortney A , Riquino Michael , Wright Kelechi
Published: March 2021

As social work educators and students, the COVID-19 pandemic impacted our teaching and learning in challenging ways. We embarked on a qualitative research study to better understand the ways in which the pandemic was affecting the social work students in our program. Three faculty mentors worked collaboratively with five social work students across BSW, MSW, and PhD programs to interview 66 BSW and MSW students about their experiences, challenges, and hopes during the early months of the pandemic. BSW and MSW students led the analysis and early dissemination for the project. This essay describes the unique experiences of social work students by using a research poem to capture the emotional and experiential aspects of the students we interviewed.
Liberation-based social work theory in progress: Time to practice what I teach
Authors: Jemal Alexis
Published: March 2021

Social work education integrates theory and practice to bridge the micro-macro divide. The theoretical framework of intersecting identities reveals hidden inequities related to health consequences. The global pandemic, reflecting a colliding of personal and professional worlds, interrupted an elective social work course designed to: 1) develop transformative potential (i.e., critical consciousness of and critical action against white supremacy, anti-blackness, and racial oppression of Black, Indigenous, People of Color (BIPOC)); 2) model liberation-based social work education and practice; and 3) prepare students to be critical social workers in the field. The pandemic created an in-class opportunity for the professor, also the course’s designer, to practice what she teaches. This self-reflexive essay details the pandemic's impact on a teaching experience and follows the professor’s journey to more fully understand systems, inequity, and her own transformative potential. The transformative potential development process included many learning experiences in the areas of relationship and community building; transformative consciousness development; accountability and responsibility; efficacy; and, critical action. The unforeseen global pandemic presented the professor with opportunities for deep reflection about liberation-based social work education and practice. By bringing the reality of how macro processes create micro consequences into the classroom in real time, the professor’s responses were tested against oppressive norms, standards and values versus those that honor a person’s humanity. An important discovery is that a critical social work educator teaches in ways that spark radical imagination to disrupt the oppressive status quo camouflaged as personal choice and business as usual.
Active learning in Japan: Breaking barriers at individual, institutional, and policy levels
Authors: Ito Hiroshi , Takeuchi Shinichi
Published: March 2021

Active learning has emerged as a means of developing generic employability skills that enable students to prepare for an uncertain future. In this context, many universities have encouraged their instructors to practice active learning. While some scholars indicate challenges with adopting this approach, few empirical studies have examined perceptions of instructors related to its application. This current study addresses this research gap—how instructors perceive the issues regarding the application of active learning—by surveying 157 instructors from across numerous institutions in Japan. Identifying issues at the individual level is important because it can provide guidance for institutional managers and policymakers on the strategies necessary for effective implementation of active learning. While the findings of this study largely confirm those of the extant literature, they also revealed which issues are more pertinent than others. Japanese instructors did not adopt active learning because they were indifferent and reluctant toward active learning and prioritized students’ knowledge acquisition over application. We conclude with a discussion of the findings and suggestions to address the perceived issues in implementing active learning at all levels, using the theory of planned behavior.
A community-health partnership response to mitigate the impact of the COVID-19 pandemic on Travellers and Roma in Ireland
Authors: Villani Jacopo , Daly Petra , Fay Ronnie , Kavanagh Lynsey , McDonagh Sandra , Amin Nurul
Published: March 2021

Irish Travellers and Roma are two ethnic minorities experiencing high levels of health inequities. These communities are at greater risk of developing COVID-19 and of suffering more severe symptoms due to poor living environments and higher rates of comorbidities. This study explores the strategies adopted by community-health partnerships and NGOs to minimise the potential widening of Travellers’ and Roma’s health inequities during the initial response to the COVID-19 pandemic in Ireland. A descriptive qualitative approach was employed to provide a detailed account of three different community and partnership-led responses. Data were gathered from multiple sources and through first-hand participation in the COVID-19 responses. Data were analysed using thematic analysis. This study found that the main pandemic mitigation interventions implemented were public health measures, culturally sensitive communications, lobbying for policy change and economic and social support. These interventions, supported by the health promotion strategies of partnership, advocacy and empowerment, have proven to be extremely important to reduce potential inequities in exposure to the virus and in access to healthcare. The findings suggest that community-health partnerships between minority groups’ organizations and healthcare professionals represent a viable approach to mitigate the disproportionate effects of a pandemic on Travellers and Roma.
Factors Influencing Creatine Kinase Response in Youth National Team Soccer Players
Authors: Schuth Gabor , Szigeti Gyorgy , Dobreff Gergely , Revisnyei Peter , Pasic Alija , Toka Laszlo , Gabbett Tim , Pavlik Gabor
Published: March 2021

Background:

Previous studies have examined the relationship between external training load and creatine kinase (CK) response after soccer matches in adults. This study aimed to build training- and match-specific CK prediction models for elite youth national team soccer players.

Hypothesis:

Training and match load will have different effects on the CK response of elite youth soccer players, and there will be position-specific differences in the most influential external and internal load parameters on the CK response.

Study Design:

Prospective cohort study.

Level of Evidence:

Level 4.

Methods:

Forty-one U16-U17 youth national team soccer players were measured over an 18-month period. Training and match load were monitored with global positioning system devices. Individual CK values were measured from whole blood every morning in training camps. The dataset consisted of 1563 data points. Clustered prediction models were used to examine the relationship between external/internal load and consecutive CK changes. Clusters were built based on the playing position and activity type. The performance of the linear regression models was described by the R2 and the root-mean-square error (RMSE, U/L for CK values).

Results:

The prediction models fitted similarly during games and training sessions ( R2 = 0.38-0.88 vs 0.6-0.77), but there were large differences based on playing positions. In contrast, the accuracy of the models was better during training sessions (RMSE = 81-135 vs 79-209 U/L). Position-specific differences were also found in the external and internal load parameters, which best explained the CK changes.

Conclusion:

The relationship between external/internal load parameters and CK changes are position specific and might depend on the type of session (training or match). Morning CK values also contributed to the next day’s CK values.

Clinical Relevance:

The relationship between position-specific external/internal load and CK changes can be used to individualize postmatch recovery strategies and weekly training periodization with a view to optimize match performance.
Adenosine-induced transient circulatory arrest in transvenous embolization of cerebral arteriovenous malformations
Authors: Ghorbani Mohammad , Griessenauer Christoph J , Wipplinger Christoph , Jabbour Pascal , Asl Mahdi Kadkhodazadeh , Rahbarian Farhad , Mortazavi Abolghasem
Published: March 2021

Due to advances in interventional techniques, the transvenous approach may present an effective treatment option for embolization of brain arteriovenous malformations (AVMs). Contrary to the transarterial method, the transvenous approach can only be utilized in a specific subset of patients and is not suitable as a standard procedure for all AVM lesions. While this technique can be helpful in certain patients, careful patient selection to ensure patient safety and favorable clinical outcomes is important. However, especially in high-flow AVMs, targeted deposition of embolic materials through a transvenous access can be challenging. Therefore, a temporary flow arrest may prove helpful. Transient cardiac arrest by use of adenosine has been applied in cerebrovascular surgery but is not common for endovascular embolization. Adenosine-induced arrest and systemic hypotension may be a feasible, safe method to reduce flow and help endovascular transvenous embolization of certain AVMs. Our study evaluated the efficiency and safety of adenosine-induced circulatory arrest for transvenous embolization of cerebral AVMs.
A case of durvalumab-induced lichenoid eruption evolving to bullous eruption after phototherapy: A case report
Authors: Manko Svitlana , Côté Benoît , Provost Nathalie
Published: March 2021

Immune checkpoint inhibitor therapy nowadays became a treatment for a wide range of cancers, and may be responsible for various dermatologic adverse effects, including bullous eruptions. In our report, we present a case of late-onset immunotherapy-induced eruption in a 62-year-old woman treated with anti-programmed cell death-L1 agent durvalumab for metastatic squamous cell carcinoma. Diagnosed as lichenoid dermatitis upon initial presentation, this eruption evolved into necrotic bullous dermatitis after several weeks of phototherapy, with histology and direct immunofluorescence study favoring lichen planus pemphigoides. Thus, this case may be regarded as durvalumab-induced lichenoid dermatitis with phototherapy-triggered progression to necrotic lichen planus pemphigoides-like eruption. The patient’s eruption responded to oral prednisone and immunotherapy interruption. Interestingly, durvalumab reintroduction in this patient led to recurrent lichenoid dermatitis without bullous component. This case of immunotherapy skin toxicity is rather distinctive by its clinical and histopathologic features, with phototherapy as an additional triggering factor.
Radiological Assessment of Postoperative Paraspinal Muscle Changes After Lumbar Interbody Fusion With or Without Minimally Invasive Techniques
Authors: Kameyama Keigo , Ohba Tetsuro , Endo Tomoka , Katsu Marina , Koji Fujita , Kensuke Koyama , Oda Kotaro , Tanaka Nobuki , Haro Hirotaka
Published: March 2021

Study Design:

Retrospective cohort study.

Background:

Percutaneous pedicle screws (PPS) have the advantage of being able to better preserve the paraspinal muscles when compared with a traditional open approach. However, the nature of changes in postoperative paraspinal muscle after damage by lumbar fusion surgery has remained largely unknown. It is clinically important to clarify and compare changes in paraspinal muscles after the various surgeries.

Objective:

(1) To determine postoperative changes of muscle density and cross-sectional area using computed tomography (CT), and (2) to compare paraspinal muscle changes after posterior lumbar interbody fusion (PLIF) with traditional open approaches and minimally invasive lateral lumbar interbody fusions (LLIF) with PPS.

Methods:

We included data from 39 consecutive female patients who underwent open PLIF and 23 consecutive patients who underwent single-staged treatment with LLIF followed by posterior PPS fixation at a single level (L4-5). All patients underwent preoperative, 6 months postoperative, and 1-year postoperative CT imaging. Measurements of the cross-sectional area (CSA) and muscle densities of paraspinal muscles were obtained using regions of interest defined by manual tracing.

Results:

We did not find any decrease of CSA in any paraspinal muscles. We did find a decrease of muscle density in the multifidus at 1 year after surgery in patients in the PILF group, but not in those in LLIF/PPS group.

Conclusions:

One year after surgery, a significant postoperative decrease of muscle density of the multifidi was observed only in patients who underwent open PLIF, but not in those who underwent LLIF/PPS.
The Effect of Prolonged Pre-Operative Halo Gravity Traction for Severe Spinal Deformities on the Cervical Spine Radiographs
Authors: Yankey Kwadwo Poku , Duah Henry Ofori , Sacramento-Domínguez Cristina , Tutu Henry Osei , Owiredu Mabel Adobea , Mahmud Rufai , Wulff Irene , Ofori-Amankwah Gerhard , Akoto Harry , Boachie-Adjei Oheneba ,   FOCOS Spine Research Group
Published: March 2021

Study Design:

Retrospective review of consecutive series.

Objective:

The study sought to assess the effect of prolonged pre-operative halo gravity traction (HGT) on the c-spine radiographs

Methods:

Data of 37 pediatric and adult patients who underwent ≥ 12wks pre-op HGT prior to definitive spine surgery from 2013-2015 at a single site in West Africa was reviewed. Radiographic assessment of the c-spine including ADI, SVA and C2-C7 Lordosis were done at pre HGT and at 4 weekly intervals. Paired T-Test was performed to evaluate changes in these parameters during HGT.

Results:

37pts, 18/19 (F/M). Average age 18.2yrs. Diagnoses: 22 idiopathic, 6 congenital, 3 Post TB, 2 NM and 4 NF. Average duration of HGT: 125 days. Baseline coronal Cobb:130 deg, corrected 30% in HGT; baseline sagittal Cobb:146 deg, corrected 32% post HGT. Baseline ADI (3.17 ± 0.63 mm) did not change at 4wks ( P > 0.05) but reduced at 8wks (2.80 ± 0.56 mm) and 12wks (2.67 ± 0.51 mm) post HGT ( P < 0.05). Baseline HGT SVA (20.7 ± 14.98 mm) significantly improved at 4wks (11.55 ± 10.26 mm), 8wks (7.54 ± 6.78 mm) and 12wks (8.88 ± 4.5 mm) ( P < 0.05). Baseline C2-C7 lordosis (43 ± 20.1 deg) reduced at 4wks (26 ± 16.37 deg), 8wks (17.8 ± 14.77 deg) and 12wks (16.7 ± 11.33 deg) post HGT ( P < 0.05). There was no incidence of atlanto-axial instability on flexion extension radiographs at any interval.

Conclusion:

Prolonged HGT, while providing partial correction of severe spine deformities, also appeared to have no adverse effect on atlanto-axial stability or cervical alignment. Therefore, HGT can be safely applied for several weeks in the preoperative management of severe spine deformities in pediatric/adult patients.
Circulating mitochondrial DNA is a proinflammatory DAMP in sickle cell disease
Authors: Tumburu Laxminath , Ghosh-Choudhary Shohini , Seifuddin Fayaz T. , Barbu Emilia A. , Yang Simon , Ahmad Maliha M. , Wilkins Lauren H. W. , Tunc Ilker , Sivakumar Ishwarya , Nichols James S. , Dagur Pradeep K. , Yang Shutong , Almeida Luis E. F. , Quezado Zenaide M. N. , Combs Christian A. , Lindberg Eric , Bleck Christopher K. E. , Zhu Jun , Shet Arun S. , Chung Jay H. , Pirooznia Mehdi , Thein Swee Lay
Published: March 2021

The pathophysiology of sickle cell disease (SCD) is driven by chronic inflammation fueled by damage associated molecular patterns (DAMPs). We show that elevated cell-free DNA (cfDNA) in patients with SCD is not just a prognostic biomarker, it also contributes to the pathological inflammation. Within the elevated cfDNA, patients with SCD had a significantly higher ratio of cell-free mitochondrial DNA (cf-mtDNA)/cell-free nuclear DNA compared with healthy controls. Additionally, mitochondrial DNA in patient samples showed significantly disproportionately increased hypomethylation compared with healthy controls, and it was increased further in crises compared with steady-state. Using flow cytometry, structured illumination microscopy, and electron microscopy, we showed that circulating SCD red blood cells abnormally retained their mitochondria and, thus, are likely to be the source of the elevated cf-mtDNA in patients with SCD. Patient plasma containing high levels of cf-mtDNA triggered the formation of neutrophil extracellular traps (NETs) that was substantially reduced by inhibition of TANK-binding kinase 1, implicating activation of the cGAS-STING pathway. cf-mtDNA is an erythrocytic DAMP, highlighting an underappreciated role for mitochondria in sickle pathology. These trials were registered at www.clinicaltrials.gov as #NCT00081523, #NCT03049475, and #NCT00047996.
Genome wide association mapping for heat tolerance in sub-tropical maize
Authors: Longmei Ningthaipuilu , Gill Gurjit Kaur , Zaidi Pervez Haider , Kumar Ramesh , Nair Sudha Krishnan , Hindu Vermuri , Vinayan Madhumal Thayil , Vikal Yogesh
Published: March 2021

Background

Heat tolerance is becoming increasingly important where maize is grown under spring season in India which coincide with grain filling stage of crop resulting in tassel blast, reduced pollen viability, pollination failure and barren ears that causes devastating yield losses. So, there is need to identify the genomic regions associated with heat tolerance component traits which could be further employed in maize breeding program.

Results

An association mapping panel, consisting of 662 doubled haploid (DH) lines, was evaluated for yield contributing traits under normal and natural heat stress conditions. Genome wide association studies (GWAS) carried out using 187,000 SNPs and 130 SNPs significantly associated for grain yield (GY), days to 50% anthesis (AD), days to 50% silking (SD), anthesis-silking interval (ASI), plant height (PH), ear height (EH) and ear position (EPO) were identified under normal conditions. A total of 46 SNPs strongly associated with GY, ASI, EH and EPO were detected under heat stress conditions. Fifteen of the SNPs was found to have common association with more than one trait such as two SNPs viz. S10_1,905,273 and S10_1,905,274 showed colocalization with GY, PH and EH whereas S10_7,132,845 SNP associated with GY, AD and SD under normal conditions. No such colocalization of SNP markers with multiple traits was observed under heat stress conditions. Haplotypes trend regression analysis revealed 122 and 85 haplotype blocks, out of which, 20 and 6 haplotype blocks were associated with more than one trait under normal and heat stress conditions, respectively. Based on SNP association and haplotype mapping, nine and seven candidate genes were identified respectively, which belongs to different gene models having different biological functions in stress biology.

Conclusions

The present study identified significant SNPs and haplotype blocks associated with yield contributing traits that help in selection of donor lines with favorable alleles for multiple traits. These results provided insights of genetics of heat stress tolerance. The genomic regions detected in the present study need further validation before being applied in the breeding pipelines.
Tolerance of Yarrowia lipolytica to inhibitors commonly found in lignocellulosic hydrolysates
Authors: Konzock Oliver , Zaghen Simone , Norbeck Joakim
Published: March 2021

Background

Lignocellulosic material is a suitable renewable carbon and energy source for microbial cell factories, such as Yarrowia lipolytica. To be accessible for microorganisms, the constituent sugars need to be released in a hydrolysis step, which as a side effect leads to the formation of various inhibitory compounds. However, the effects of these inhibitory compounds on the growth of Y. lipolytica have not been thoroughly investigated.

Results

Here we show the individual and combined effect of six inhibitors from three major inhibitor groups on the growth of Y. lipolytica. We engineered a xylose consuming strain by overexpressing the three native genes XR, XDH, and XK and found that the inhibitor tolerance of Y. lipolytica is similar in glucose and in xylose. Aromatic compounds could be tolerated at high concentrations, while furfural linearly increased the lag phase of the cultivation, and hydroxymethylfurfural only inhibited growth partially. The furfural induced increase in lag phase can be overcome by an increased volume of inoculum. Formic acid only affected growth at concentrations above 25 mM. In a synthetic hydrolysate, formic acid, furfural, and coniferyl aldehyde were identified as the major growth inhibitors.

Conclusion

We showed the individual and combined effect of inhibitors found in hydrolysate on the growth of Y. lipolytica. Our study improves understanding of the growth limiting inhibitors found in hydrolysate and enables a more targeted engineering approach to increase the inhibitor tolerance of Y. lipolytica. This will help to improve the usage of Y. lipolytica as a sustainable microbial cell factory.
Improved node culture methods for rapid vegetative propagation of switchgrass (Panicum virgatum L.)
Authors: Wang Yongqin , Dong Weihong , Saha Malay C. , Udvardi Michael K. , Kang Yun
Published: March 2021

Background

Switchgrass (Panicum virgatum L.) is an important bioenergy and forage crop. The outcrossing nature of switchgrass makes it infeasible to maintain a genotype through sexual propagation. Current asexual propagation protocols in switchgrass have various limitations. An easy and highly-efficient vegetative propagation method is needed to propagate large natural collections of switchgrass genotypes for genome-wide association studies (GWAS).

Results

Micropropagation by node culture was found to be a rapid method for vegetative propagation of switchgrass. Bacterial and fungal contamination during node culture is a major cause for cultural failure. Adding the biocide, Plant Preservative Mixture (PPM, 0.2%), and the fungicide, Benomyl (5 mg/l), in the incubation solution after surface sterilization and in the culture medium significantly decreased bacterial and fungal contamination. In addition, “shoot trimming” before subculture had a positive effect on shoot multiplication for most genotypes tested. Using the optimized node culture procedure, we successfully propagated 330 genotypes from a switchgrass GWAS panel in three separate experiments. Large variations in shoot induction efficiency and shoot growth were observed among genotypes. Separately, we developed an in planta node culture method by stimulating the growth of aerial axillary buds into shoots directly on the parent plants, through which rooted plants can be generated within 6 weeks. By circumventing the tissue culture step and avoiding application of exterior hormones, the in planta node culture method is labor- and cost-efficient, easy to master, and has a high success rate. Plants generated by the in planta node culture method are similar to seedlings and can be used directly for various experiments.

Conclusions

In this study, we optimized a switchgrass node culture protocol by minimizing bacterial and fungal contamination and increasing shoot multiplication. With this improved protocol, we successfully propagated three quarters of the genotypes in a diverse switchgrass GWAS panel. Furthermore, we established a novel and high-throughput in planta node culture method. Together, these methods provide better options for researchers to accelerate vegetative propagation of switchgrass.
Use of volume controlled vs. pressure controlled volume guaranteed ventilation in elderly patients undergoing laparoscopic surgery with laryngeal mask airway
Authors: Wang Ping , Zhao Shihao , Gao Zongbin , Hu Jun , Lu Yao , Chen Jinbao
Published: March 2021

Background

The peak inspiratory pressure (PIP) is crucial in mechanical ventilation with supraglottic airway device (SAD). Pressure-controlled ventilation volume-guaranteed (PCV-VG), delivering a preset tidal volume with the lowest required airway pressure, is being increasingly used during general anesthesia. In this study, we compared respiratory mechanics and circulatory parameters between volume-controlled ventilation (VCV) and PCV-VG in elderly patients undergoing laparoscopic surgery using the laryngeal mask airway supreme (LMA).

Methods

Eighty participants scheduled for laparoscopic surgery were enrolled in this prospective, randomized clinical trial. The participants were randomly assigned to receive VCV or PCV-VG. PIP, dynamic compliance (Cdyn) and mean inspiratory pressure (Pmean) were recorded at 5 min after induction of anesthesia (T1), 5 min after pneumoperitoneum(T2), 30 and 60 min after pneumoperitoneum (T3 and T4). Data including other respiratory variables, hemodynamic variables, and arterial blood gases were also collected. The difference in PIP between VCV and PCV-VG was assessed as the primary outcome.

Results

PIP was significantly lower at T2, T3, and T4 in both groups compared with T1 (allP <  0.0001), and it was significantly lower in the PCV-VG group than the VCV group at T2, T3, and T4 (allP <  0.001). Cydn was decreased at T2, T3, and T4 in two groups compared with T1 (allP <  0.0001), but it was higher in PCV-VG group than in VCV group at T2, T3, and T4 (allP <  0.0001). There were on statistically significant differences were found between the groups for other respiratory and hemodynamic variables.

Conclusion

In elderly patients who underwent laparoscopic surgery using an LMA, PCV-VG was superior to VCV in its ability to provide ventilation with lower peak inspiratory pressure and greater dynamic compliance.
Multi-centre implementation of an Educational program to improve the Cardiac Arrest diagnostic accuracy of ambulance Telecommunicators and survival outcomes for sudden cardiac arrest victims: the EduCATe study design and methodology
Authors: Vaillancourt Christian , Charette Manya , Naidoo Sarika , Taljaard Monica , Church Matthew , Hodges Stephanie , Leduc Shannon , Christenson Jim , Cheskes Sheldon , Dainty Katie , Feldman Michael , Goldstein Judah , Tallon John , Helmer Jennie , Sibley Aaron , Spidel Matthew , Blanchard Ian , Garland Jim , Cyr Kathryn , Brehaut Jamie , Dorian Paul , Lacroix Colette , Zambon Sandra , Thiruganasambandamoorthy Venkatesh
Published: March 2021

Background

Sudden cardiac death remains a leading cause of mortality in Canada, resulting in more than 35,000 deaths annually. Most cardiac arrest victims collapse in their own home (85% of the time) and 50% are witnessed by a family member or bystander. Survivors have a quality of life similar to the general population, but the overall survival rate for out-of-hospital cardiac arrest (OHCA) rarely exceeds 8%. Victims are almost four times more likely to survive when receiving bystander CPR, but bystander CPR rates have remained low in Canada over the past decade, not exceeding 15–25% until recently. Telecommunication-assisted CPR instructions have been shown to significantly increase bystander CPR rates, but agonal breathing may be misinterpreted as a sign of life by 9–1-1 callers and telecommunicators, and is responsible for as much as 50% of missed OHCA diagnoses.

We sought to improve the ability and speed with which ambulance telecommunicators can recognize OHCA over the phone, initiate timely CPR instructions, and improve survival.

Methods

In this multi-center national study, we will implement and evaluate an educational program developed for ambulance telecommunicators using a multiple baseline interrupted time-series design. We will compare outcomes 12 months before and after the implementation of a 20-min theory-based educational video addressing barriers to recognition of OHCA while in the presence of agonal breathing. Participating Canadian sites demonstrated prior ability to collect standardized data on OHCA. Data will be collected from eligible 9–1-1 recordings, paramedic documentation and hospital medical records. Eligible cases will include suspected or confirmed OHCA of presumed cardiac origin in patients of any age with attempted resuscitation.

Discussion

The ability of telecommunication-assisted CPR instructions to improve bystander CPR and survival rates for OHCA victims is undeniable. The ability of telecommunicators to recognize OHCA over the phone is unequivocally impeded by relative lack of training on agonal breathing, and reluctance to initiate CPR instructions when in doubt. Our pilot data suggests the potential impact of this project will be to increase absolute OHCA recognition and bystander CPR rates by at least 10%, and absolute out-of-hospital cardiac arrest survival by 5% or more.

Trial registration

Prospectively registered on March 28, 2019 at ClinicalTrials.gov identifier: NCT03894059.
The impact of symptoms on quality of life before and after diagnosis of coeliac disease: the results from a Polish population survey and comparison with the results from the United Kingdom
Authors: Majsiak Emilia , Choina Magdalena , Golicki Dominik , Gray Alastair M. , Cukrowska Bożena
Published: March 2021

Background

Coeliac disease (CD) is characterised by diverse clinical symptoms, which may cause diagnostic problems and reduce the patients’ quality of life. A study conducted in the United Kingdom (UK) revealed that the mean time between the onset of coeliac symptoms and being diagnosed was above 13 years. This study aimed to analyse the diagnostic process of CD in Poland and evaluate the quality of life of patients before and after CD diagnosis. In addition, results were compared to the results of the original study conducted in the UK.

Methods

The study included 2500 members of the Polish Coeliac Society. The patients were asked to complete a questionnaire containing questions on socio-demographic factors, clinical aspects and quality of life, using the EQ-5D questionnaire. Questionnaires received from 796 respondents were included in the final analysis.

Results

The most common symptoms reported by respondents were bloating (75%), abdominal pain (72%), chronic fatigue (63%) and anaemia (58%). Anaemia was the most persistent symptom, with mean duration prior to CD diagnosis of 9.2 years, whereas diarrhoea was observed for the shortest period (4.7 years). The mean duration of any symptom before CD diagnosis was 7.3 years, compared to 13.2 years in the UK. CD diagnosis and the introduction of a gluten-free diet substantially improved the quality of life in each of the five EQ-5D-5L health dimensions: pain and discomfort, anxiety and depression, usual activities, self-care and mobility (p < 0.001), the EQ-Index by 0.149 (SD 0.23) and the EQ-VAS by 30.4 (SD 28.3) points.

Conclusions

Duration of symptoms prior to the diagnosis of CD in Poland, although shorter than in the UK, was long with an average of 7.3 years from first CD symptoms. Faster CD diagnosis after the onset of symptoms in Polish respondents may be related to a higher percentage of children in the Polish sample. Introduction of a gluten-free diet improves coeliac patients’ quality of life. These results suggest that doctors should be made more aware of CD and its symptoms across all age groups.
Development and validation of a simple-to-use nomogram to predict liver metastasis in patients with pancreatic neuroendocrine neoplasms: a large cohort study
Authors: Pan Maoen , Yang Yuanyuan , Teng Tianhong , Lu Fengchun , Chen Yanchan , Huang Heguang
Published: March 2021

Background

Liver metastasis is an important prognostic factor for pancreatic neuroendocrine neoplasms (pNENs), but the relationship between the clinical features of patients with pNEN and liver metastasis remains undetermined. The aim of this study was to establish and validate an easy-to-use nomogram to predict liver-metastasis in patients with pNEN.

Methods

We obtained the clinicopathologic data of 2960 patients with pancreatic neuroendocrine neoplasms from the Surveillance, Epidemiology and End Results (SEER) database between 2010 and 2016. Univariate and multivariate logistic regression were done to screen out independent influencing factors to establish the nomogram. The calibration plots and the area under the receiver operating characteristic curve (AUC) were used to evaluate the performance of nomogram. Decision curve analysis (DCA) was applied to compare the novel model with the conventional predictive methods.

Results

A total of 2960 patients with pancreatic neuroendocrine neoplasms were included in the study. Among these, 1974 patients were assigned to the training group and 986 patients to the validation group. Multivariate logistic regression identified, tumor size, grade, other site metastasis, T stage and N stage as independent risk factors. The calibration plot showed good discriminative ability in the training and validation groups, with C-indexes of 0.850 for the training cohort and 0.846 for the validation cohort. The AUC values were 0.850 (95% CI 0.830–0.869) and 0.839 (95% CI 0.812–0.866), respectively. The nomogram total points (NTP) had the potential to stratify patients into low risk, medium risk and high risk (P < 0.001). Finally, comparing the nomogram with traditional prediction methods, the DCA curve showed that the nomogram had better net benefit.

Conclusions

Our nomogram has a good ability to predict liver metastasis of pancreatic neuroendocrine neoplasms, and it can guide clinicians to provide suitable prevention and treatment measures for patients with medium- and high-risk liver metastasis.
Association between in-hospital frailty and health-related quality of life after stroke: the Nor-COAST study
Authors: Wæhler Idunn Snorresdatter , Saltvedt Ingvild , Lydersen Stian , Fure Brynjar , Askim Torunn , Einstad Marte Stine , Thingstad Pernille
Published: March 2021

Background

Stroke survivors are known to have poorer health-related quality of life (HRQoL) than the general population, but less is known about characteristics associated with HRQoL decreasing through time following a stroke. This study aims to examine how in-hospital frailty is related to HRQoL from 3 to 18 months post stroke.

Method

Six hundred twenty-five participants hospitalised with stroke were included and followed up at 3 and/or 18 months post stroke. Stroke severity was assessed the day after admission with the National Institutes of Health Stroke Scale (NIHSS). A modified Fried phenotype was used to assess in-hospital frailty; measures of exhaustion, physical activity, and weight loss were based on pre-stroke status, while gait speed and grip strength were measured during hospital stay. HRQoL at 3- and 18-months follow-up were assessed using the five-level version of the EuroQol five-dimensional descriptive system (EQ-5D-5L) and the EuroQol visual analogue scale (EQ-5D VAS). We conducted linear mixed effect regression analyses unadjusted and adjusted for sex, age, and stroke severity to investigate the association between in-hospital frailty and post-stroke HRQoL.

Results

Mean (SD) age was 71.7 years (11.6); mean NIHSS score was 2.8 (4.0), and 263 (42.1%) were female. Frailty prevalence was 10.4%, while 58.6% were pre-frail. The robust group had EQ-5D-5L index and EQ-5D VAS scores at 3 and 18 months comparable to the general population. Also at 3 and 18 months, the pre-frail and frail groups had significantly lower EQ-5D-5L indices than the robust group (p <  0.001), and the frail group showed a larger decrease from 3 to 18 months in the EQ-5D-5L index score compared to the robust group (− 0.056; 95% CI − 0.104 to − 0.009; p = 0.021). There were no significant differences in change in EQ-5D VAS scores between the groups.

Conclusion

This study on participants mainly diagnosed with mild strokes suggests that robust stroke patients have fairly good and stable post-stroke HRQoL, while post-stroke HRQoL is impaired and continues to deteriorate among patients with in-hospital frailty. This emphasises the importance of a greater focus on frailty in stroke units.

Trial registration

ClinicalTrials.gov (NCT02650531).
Integrated bioinformatic analysis identifies UBE2Q1 as a potential prognostic marker for high grade serous ovarian cancer
Authors: Topno Rachel , Singh Ibha , Kumar Manoj , Agarwal Pallavi
Published: March 2021

Background

High grade serous ovarian cancer (HGSOC) accounts for nearly 60% of total cases of epithelial ovarian cancer. It is the most aggressive subtype, which shows poor prognosis and low patient survival. For better management of HGSOC patients, new prognostic biomarkers are required to facilitate improved treatment strategies and ensure suitable healthcare decisions.

Methods

We performed genome wide expression analysis of HGSOC patient samples to identify differentially expressed genes (DEGs) using R based Limma package, Clust and other statistical tools. The identified DEGs were subjected to weighted gene co-expression network analysis (WGCNA) to identify co-expression patterns of relevant genes. Module trait and gene ontology analyses were performed to establish important gene co-expression networks and their biological functions. Overlapping the most relevant DEG cluster 4 with prominent WGCNA cyan module identified strongest correlation of UBE2Q1 with ovarian cancer and its prognostic significance on survival probability of ovarian cancer patients was investigated. The predictive value of UBE2Q1 as a potential biomarker was analysed by correlating its expression with 12-months relapse free survival of patients in response to platin/taxane, the standard first-line chemotherapy for ovarian cancer, and analysing area under the ROC curve.

Results

An integrated gene expression analysis and WGCNA, identified UBE2Q1 as a potential prognostic marker associated with poor relapse-free survival and response outcome to platin/taxane treatment of patients with high grade serous ovarian cancer.

Conclusions

Our study identifies a potential UBE2Q1 – B4GALT3 functional axis in ovarian cancer, where only the E2 conjugating enzyme showed a poor prognostic impact on the disease.
Etiologic distribution and clinical characteristics of pediatric diabetes in 276 children and adolescents with diabetes at a single academic center
Authors: Kim Ja Hye , Lee Yena , Choi Yunha , Kim Gu-Hwan , Yoo Han-Wook , Choi Jin-Ho
Published: March 2021

Background

The prevalence of monogenic diabetes is estimated to be 1.1–6.3% of patients with diabetes mellitus (DM) in Europe. The overlapping clinical features of various forms of diabetes make differential diagnosis challenging. Therefore, this study investigated the etiologic distribution and clinical characteristics of pediatric diabetes, including monogenic diabetes, who presented at a single tertiary center over the last 20 years.

Methods

This study included 276 consecutive patients with DM diagnosed before 18 years of age from January 2000 to December 2019 in Korea. Clinical features, biochemical findings, β-cell autoantibodies, and molecular characteristics were reviewed retrospectively.

Results

Of the 276 patients, 206 patients (74.6%), 49 patients (17.8%), and 21 patients (7.6%) were diagnosed with type 1 DM, type 2 DM, and clinically suspected monogenic diabetes, respectively. Among 21 patients suspected to have monogenic diabetes, 8 patients had clinical maturity-onset diabetes of the young (MODY), and the remaining 13 patients had other types of monogenic diabetes. Among them, genetic etiologies were identified in 14 patients (5.1%) from 13 families, which included MODY 5, transient neonatal DM, developmental delay, epilepsy, and neonatal diabetes (DEND) syndrome, Wolfram syndrome, Donohue syndrome, immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome, Fanconi-Bickel syndrome, Wolcott-Rallison syndrome, cystic fibrosis-related diabetes, and maternally inherited diabetes and deafness.

Conclusions

Genetically confirmed monogenic diabetes accounted for 5.1% of patients evaluated at a single tertiary center over 20-year period. Based on the findings for our sample, the frequency of mutations in the major genes of MODY appears to be low among pediatric patients in Korea. It is critical to identify the genetic cause of DM to provide appropriate therapeutic options and genetic counseling.
Predictors of a placebo response in patients with hand osteoarthritis: post-hoc analysis of two randomized controlled trials
Authors: Park Jin Kyun , Ahn Se Han , Shin Kichul , Lee Yun Jong , Song Yeong Wook , Lee Eun Bong
Published: March 2021

Background

Placebo can have a significant therapeutic effect in patients with hand osteoarthritis (OA). This aim of the study is to identify factors associated with a clinically meaningful placebo response in patients with hand OA.

Methods

This post-hoc analysis of two double-blind, placebo-controlled, randomized trials (RCTs) investigating the efficacy of GCSB-5 or diacerein as treatments for hand OA analyzed the efficacy of a placebo. Clinical and laboratory factors associated with a clinically meaningful response, defined as an improvement in the Australian/Canadian Osteoarthritis Hand Index (AUSCAN) pain score > 10 at 4 weeks relative to baseline, were identified.

Results

The mean improvement in the AUSCAN pain score was − 6.0 ± 20.3, with marked variation between 143 hand OA patients (range: − 76.4 to 33.2). A clinically meaningful improvement was observed in 54 (37.8%) patients. Placebo responders had worse AUSCAN pain scores (55.7 ± 19.7 vs. 43.6 ± 21.6, p = 0.001) and a worse AUSCAN stiffness (68.2 ± 20.5 vs. 57.5 ± 24.5, p = 0.008) at baseline than non-responders. Improvements in pain correlated with the baseline pain level (Pearson r = − 427, p < 0.001). Structural joint changes such as tender, swollen, enlarged, or deformed joint counts did not differ between placebo responders and non-responders. In a multivariable analysis, only baseline AUSCAN pain was associated with a clinically meaningful placebo response (OR: 1.054, 95% CI [1.019–1.089], p = 0.002).

Conclusions

High levels of pain at baseline are predictive of a clinically meaningful placebo response in patients with hand OA. Further studies are needed to optimize and utilize the benefit of placebo responses in patients with hand OA.