bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the mutant selection window of danofloxacin against Actinobacillus pleuropneumoniae in an in vitro dynamic model
Authors: Zhang Longfei , Wang Hongjuan , Bai Yilin , Wang Lei , Bai Yueyu , Hu Jianhe
Published: January 2023

Introduction

The rapid emergence and widespread spread of multidrug-resistant bacteria is a serious threat to the health of humans and animals. The pharmacokinetic/pharmacodynamic (PK/PD) integration model based on mutant selection window (MSW) theory is an important method to optimize the dosage regimen to prevent the emergence and spread of drug-resistant bacteria. Actinobacillus pleuropneumoniae (AP) is a pathogen that can cause pleuropneumonia in pigs.

Methods

We employed an in vitro dynamic infection model (DIM) to study the prevention of drug-resistant mutations of danofloxacin against AP. A peristaltic pump was applied to establish an in vitro DIM to simulate the PK of danofloxacin in plasma, and to study the MSW of danofloxacin against AP. A peristaltic-pump in vitro infection model was established to simulate dynamic changes in the danofloxacin concentration in pig plasma. PK and PD data were obtained. Then, the relationship between PK/PD parameters and antibacterial activity was analyzed by the sigmoid Emax model.

Results and discussion

The area under the curve during 24 h/ the minimum concentration that inhibits colony formation by 99% (AUC24h/MIC99) had the best-fitting relationship with antibacterial activity. The AUC24h/MIC99 values for a bacteriostatic effect, bactericidal effect, and eradication effect were 2.68, 33.67, and 71.58 h, respectively. We hope these results can provide valuable guidance when using danofloxacin to treat AP infection.
Assessing the Impact of Universities’ Entrepreneurial Activity on Regional Competitiveness
Authors: Brás Gonçalo Rodrigues , Torres Preto Miguel , Daniel Ana Dias , Teixeira Aurora A. C.
Published: January 2023

The aim of this study is to test the multidimensional construct of the Entrepreneurial University (EU), and therefore to confirm whether EU factors make a positive contribution to regional competitiveness. Data were collected from ten Portuguese Public Universities (PPUs) through a self-administered questionnaire. First- and second-order confirmatory factor analyses (CFA) were performed through factor and multiple linear regression analyses. The main findings show that EU related factors—perceived and combined with actual regional metrics—especially entrepreneurial supporting measures, positively contributed to regional competitiveness. This study shows policy makers that universities are not merely cost centres but provide knowledge spillovers that can have a positive influence on regional competitiveness.
Generation Z “Life Skills” Acquired and Enhanced through Internships before and during COVID-19 Pandemic
Authors: Shtembari Eriona , Elgün Riza Feridun
Published: January 2023

The paper explores “life skills” acquired and enhanced during an internship in preparation for the work of the future, identifies challenges faced and gaps between academic preparation and internship requirements, and explores the impact of the COVID 19 pandemic on the internship experiences of Generation Z students. This qualitative study involves cohorts from 2016–2021, including 50 students before the pandemic, and 47 students during the pandemic, majoring in the field of Economy and Business. Data are gathered through 97 semi-structured interviews and written reports from the students. Interestingly, the findings showed that job placement is not the main reason why students pursue an internship; acquiring skills and experience was identified as the major motivating factor. “Communication” was the top enhanced skill from internships in both periods before and during the pandemic, while “relationship building” and “ethical issues” ranked as the second and third most important skills before the pandemic, to be replaced by “critical-thinking and problem-solving” and “analytical and technical” skills during the pandemic. Lack of accountability and COVID-19 stress were reported as the main challenges experienced during internships, while lacking hands-on experience and social skills were identified as gaps. The foremost pieces of advice for future interns revealed in this study are to get involved seriously and show respect and integrity.
Grape Wine Cultivation Carbon Footprint: Embracing a Life Cycle Approach across Climatic Zones
Authors: Hefler Yuval Tamar , Kissinger Meidad
Published: January 2023

Ongoing climate change processes and increasing environmental pressure suggest the need to adjust the wine production systems worldwide to the new conditions while reducing their environmental pressure. The grapes’ cultivation phase may be influenced by expected changes. It follows that existing grape wine cultivation systems should be analyzed to identify major ‘hotspots’ and opportunities for change. Several studies have analyzed materials, energy inputs, and related emissions along the grape wine life cycle. However, most research focuses on traditional grape wine growing areas, and no study has yet focused on grape wine grown in unconventional desert areas. The research presented in this paper analyzed the carbon footprint (CF) of grapes grown in the Mediterranean, semi-arid, and arid climatic regions in the state of Israel. It revealed that, on average, a ton of grapes generates 342 kg CO2 eq from the cradle to the farm gate. The product was analyzed using a life-cycle approach, with the aim of studying the CF of each phase according. Most emissions were found to be related to the use of fertilizers (37%), fuel for transportation and mechanization (19%), and water supply (17%). The CF of grapes in the arid region was found to be the highest at 361 kg CO2 eq compared to 317 kg CO2 eq in the semi-arid region and 346 kg CO2 eq in the Mediterranean region. The analysis emphasizes the arid and semi-arid potential to reduce its CF by implementing farm management practices, including the choice of grape varieties, changing vineyard infrastructure, fertilizers, water management, and more. As presented here, understanding cropping systems in these regions can promote a better adaptation of the cropping systems to the changing conditions around the world.
Agricultural Transformation in the Rural Farmer Communities of Stung Chrey Bak, Kampong Chhnang Province, Cambodia
Authors: Kimkong Ham , Promphakping Buapun , Hudson Harri , Day Samantha C. J.
Published: January 2023

This paper examines the processes of agricultural transformation and their impacts within six rice farming communities in Cambodia. For this, we explored four drivers of agricultural transformation: (1) market integration, (2) modern technologies, (3) household assets, and (4) institutional-policy processes. The paper employs qualitative methods, using document analysis on the policy literature and datasets, field observations, focus groups, and key informant interviews in six rice farming communities in the Stung Chrey Bak Commune, Kampong Chhnang Province. Herein, we analyze the processes of agricultural transformation that shape farmer livelihoods and contribute to the literature regarding the dynamic and uneven politics of implementing the green revolution. Our findings show that agricultural transformation in the six rice farming communities has had mixed results. While the transitions have improved household income, they have also led to insecurity, with potential impacts on the long-term sustainability of the rice-production sector. These include higher input costs, fluctuating rice revenues, and environmental impacts from increased chemical use. We show that greater support is needed in these farming communities in order to achieve sustainable rice production going forward, particularly in light of climate change, indebtedness, and the migration of young labor to off-farm employment, leading to aging farmer populations.
Cold Tolerance Mechanisms in Mungbean (Vigna radiata L.) Genotypes during Germination
Authors: Manasa Lekshmi S. , Panigrahy Madhusmita , Panigrahi Kishore Chandra , Mishra Gayatri , Panda Sanjib Kumar , Rout Gyana Ranjan
Published: January 2023

Mungbean or greengram (Vigna radiata) is an important legume crop well known for its high protein with nitrogen-fixing abilities. However, the severe yield loss in mungbean occurs due to susceptibility to low temperatures at all stages of plant growth including germination and is a serious concern for its cultivation and productivity. To select cold-tolerant genotypes, a germination-based screening at 10 °C was performed in a total of 204 germplasms. The study showed that cold stress of the initial 8-days during seedling establishment imposed a negative impact throughout the life of mungbean genotypes, which were reflected in the vegetative and reproductive phase (plant height, days to 50% flowering and pods/plant, seeds/pod, yield/plant, and 100-seed weight). The biplot analysis showed that parameters such as germination rate index, Timson’s index, mean germination time, and coefficient of the velocity of germination are the key influential germination parameters for identifying cold tolerance in the seedling stage. Identified cold-tolerant genotype (PAU911) retained higher rootlet number, leaf area, and increased chlorophyll, carotenoid, and malondialdehyde (MDA) content at 10 °C. Based on the confocal microscopic study, it is noticed that the stomatal density, open pore percentage, and trichome density were significant differences in seedlings exposed to cold stress as compared to non-stress. On the basis of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis, it is observed that a new protein identified as TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE1 (TTL1) (UNIPROT Identifier: LOC106762419) which highly correlated with the cold stress response of in the cold-tolerant genotype. Our study identifies a noble member, TTL1, whose expression has a positive role in cold tolerance response at the protein level in V. radiata. This study will help breeding programs with regard to the sustainable growth of mungbean.
The Influence of Konik Horses Grazing and Meteorological Conditions on Wetland Communities
Authors: Chodkiewicz Anna , Stypiński Piotr , Studnicki Marcin , Borawska-Jarmułowicz Barbara
Published: January 2023

Extensive management is one of the methods used in the conservation of fen meadows. The aim of the study was to characterize grassland communities grazed by Konik horses in the Biebrza National Park and to assess the influence of short-term grazing on the sward. During a 3-year period, phytosociological studies, measurements of the sward cover and height, as well as an assessment of the botanical composition of meadows were conducted in places grazed and excluded from grazing. The grasslands were mowed once a year at the turn of July and August, with the exception of mid-forest meadows. The area studied was dominated by communities of the Molinion alliance. Horses kept at a very low stocking rate reduced the height and cover of the sward. In the short-term, weather conditions, especially the duration of floods, had an even greater impact on the species composition of communities than grazing. Therefore, in order to protect the Molinia meadows, apart from extensive mowing, it is necessary to maintain appropriate habitat conditions, especially in terms of controlling the moisture, and conduct its regular monitoring.
Drying Process Modeling and Quality Assessments Regarding an Innovative Seed Dryer
Authors: Arsenoaia Vlad Nicolae , Roșca Gheorghe Radu , Cârlescu Petru , Băetu Marius , Rațu Roxana , Veleșcu Ionuț , Țenu Ioan
Published: January 2023

The dehydration of agricultural products is a topic of research covering the preservation and conservation of the quality of agricultural and food items. The actual tasks of environmental protection include creating novel solutions to reduce the energy needs and exothermic consequences of the bulk of industrial processes. Due to the fact that air temperature and velocity in the seed layer are not uniform, the currently employed seed dryers have significant energy requirements and generate a lot of heat. A device that addresses this problem was developed and is now the subject of a patent application. The Coandă effect-based tronconic plates used in the dryer enable warm air to be dispersed uniformly throughout the product mass. Mathematical modeling was used for the design, operation, and optimization of the baffled drying unit. The investigations, which were conducted by modifying and observing the operational parameters (the velocity and temperature of the warm air) used four distinct types of seed with three different beginning moisture concentrations. Following the completion of the CFD simulation, the unit’s design was created in SolidWorks. The seed and air humidity were measured using sensors, and the air speed was measured using an anemometer. The current lines and fields were used to express the speed and temperature results. The unit with deflectors reduced the amount of heat released by up to 15.38% and reduced the amount of energy used by up to 14%.
PlantStereo: A High Quality Stereo Matching Dataset for Plant Reconstruction
Authors: Wang Qingyu , Wu Dihua , Liu Wei , Lou Mingzhao , Jiang Huanyu , Ying Yibin , Zhou Mingchuan
Published: January 2023

Stereo matching is a depth perception method for plant phenotyping with high throughput. In recent years, the accuracy and real-time performance of the stereo matching models have been greatly improved. While the training process relies on specialized large-scale datasets, in this research, we aim to address the issue in building stereo matching datasets. A semi-automatic method was proposed to acquire the ground truth, including camera calibration, image registration, and disparity image generation. On the basis of this method, spinach, tomato, pepper, and pumpkin were considered for experiment, and a dataset named PlantStereo was built for reconstruction. Taking data size, disparity accuracy, disparity density, and data type into consideration, PlantStereo outperforms other representative stereo matching datasets. Experimental results showed that, compared with the disparity accuracy at pixel level, the disparity accuracy at sub-pixel level can remarkably improve the matching accuracy. More specifically, for PSMNet, the EPE and bad−3 error decreased 0.30 pixels and 2.13%, respectively. For GwcNet, the EPE and bad−3 error decreased 0.08 pixels and 0.42%, respectively. In addition, the proposed workflow based on stereo matching can achieve competitive results compared with other depth perception methods, such as Time-of-Flight (ToF) and structured light, when considering depth error (2.5 mm at 0.7 m), real-time performance (50 fps at 1046 × 606), and cost. The proposed method can be adopted to build stereo matching datasets, and the workflow can be used for depth perception in plant phenotyping.
Biomass Fuel Production through Cultivation of Microalgae Coccomyxa dispar and Scenedesmus parvus in Palm Oil Mill Effluent and Simultaneous Phycoremediation
Authors: Ooi Wen Ching , Dominic Debbie , Kassim Mohd Asyraf , Baidurah Siti
Published: January 2023

Palm oil mill effluent (POME) is a potential alternative sources of biomass fuel upon phycoremediation treatment using microorganisms. In this study, Coccomyxa dispar and Scenedesmus parvus, as acidophilic microalgae, were used to investigate growth and the production of biomass fuel from the cultivation of POME, as well the effectiveness of removing contaminants from POME. Individual cultivation was conducted at 26 ± 3 °C for 14 days under three growth modes (mixotrophic, heterotrophic, and autotrophic). To elucidate the potential phycoremediation properties, the characteristics of treated POME were compared, such as optical density (OD), cell dry weight (CDW), calorific energy values (CEV), chemical oxygen demand (COD), biochemical oxygen demand (BOD), carbon, hydrogen, and nitrogen (CHN) elemental analysis, including oil and grease content. S. parvus exhibits an outstanding growth profile for all growth modes compared to C. dispar, with measurements of 228.8, 37.08, and 118.2 mg/L observed at day 14 of cultivation. The highest CEV is 32.30 MJ/kg, which was obtained from S. parvus in the mixotrophic mode. Maximum removal efficiency for COD and BOD was 81% and 19% in the mixotrophic growth mode with S. parvus. These results pinpoint that S. parvus has the potential to be utilized for biomass fuel production with high CEV and effective POME phycoremediation.
Salinity Threshold of Tall Wheatgrass for Cultivation in Coastal Saline and Alkaline Land
Authors: Li Hongwei , Li Wei , Zheng Qi , Zhao Maolin , Wang Jianlin , Li Bin , Li Zhensheng
Published: January 2023

Tall wheatgrass (Elytrigia elongata) has the potential to be utilized on marginal land, such as coastal saline-alkaline soils, to meet rising ruminant feed demand. However, the salinity threshold for cultivation of tall wheatgrass remains unclear, which restricts its extensive application. Here, a tall wheatgrass line, Zhongyan 1, was grown in saline-alkaline soils in the Yellow River Delta region to determine its salinity threshold. The results showed that the soil salinity of AM = 1.23, measured with a PNT3000 activity meter, led to only 5% dead plants of tall wheatgrass. Four grades of seedling plants were classified according to the morphological response of Zhongyan 1 to saline soils. The soil salinity declined while the survival rate and forage yield increased from grade 1 to grade 4 plants. Plant height and dry matter yield were negatively related to soil salinity. When the salinity in the soil depth of 0–10 cm was over 1%, the survival rate of tall wheatgrass declined dramatically with the increase in soil salinity. Under saline-alkaline stress, the plant height during 12–31 May was positively related to forage yield, which can be used as an indicator of productivity. The tall type (70–120 cm) produced 5627.2 kg ha−1 of dry matter, which was 3.32 times that of the dwarf type (20–69 cm). The forage yield of tall wheatgrass in saline-alkaline land was largely affected by the proportion of highly saline soil. Collectively, the soil salinity of 1% at a depth of 0–10 cm and the AM values of 1.23 measured with a PNT3000 activity meter can be used as the salinity threshold for cultivation of tall wheatgrass in coastal saline-alkaline land.
Rice Production Chain: Environmental and Social Impact Assessment—A Review
Authors: Vinci Giuliana , Ruggieri Roberto , Ruggeri Marco , Prencipe Sabrina Antonia
Published: January 2023

Rice is the most widely used cereal for human consumption, attributing its production as one of the most important activities for the global population. Therefore, given its economic and nutritional value, assessing the sustainability of this production process could be worth noting. In this regard, this research aims to investigate the most recent literature related to Life Cycle Assessment (LCA) of rice primary production, to clarify the extent to which Life Cycle Thinking (LCT) and thus the three pillars of sustainability have been applied in the rice sector, as well as to highlight possible research gaps. Thus, 40 articles (2012–2022) were analyzed. The main research gaps that were found were, firstly, that there was a lesser tendency to consider multiple functional units, highlighting how little multifunctionality is considered. As to be expected, there was also a great difference in methodological choices, which often leads to a great variability of results, making evaluations and comparisons of impacts uncertain. These were also highly dependent on soil and climate conditions in the various countries, which could in turn affect input utilization, and results. The study of the impacts of primary rice production was then addressed by a few countries, among which some of the largest producers were absent, while the least considered aspects were related to the depletion of abiotic resources and the promotion of organic farming. Finally, sustainability assessments in rice production had little focus on the socio-economic dimension, showing how little LCT is considered. Therefore, based on this consideration, a Social Life Cycle Assessment was integrated into the study, the results of which show that the countries with medium to high social impacts could be India, Sri Lanka, Thailand, and Bangladesh.
Soil Microbes from Saline–Alkali Farmland Can Form Carbonate Precipitates
Authors: Liu Zhen , Li Jing , Zhang Yitao , Gong Huarui , Hou Ruixing , Sun Zhigang , Ouyang Zhu
Published: January 2023

The formation of soil inorganic carbon in saline–alkali lands is of great significance for enhancing soil carbon sequestration. As for the formation mechanisms, in addition to the discovered abiotic mechanisms, the microbial mechanisms remain unclear. To address this, soil microbes were isolated from the saline–alkali farmland of the Yellow River Delta in north China. Then, their capacity for carbonate precipitation formation was determined. Ten microbial strains were obtained from the soil. Of these, seven strains (four bacterial strains and three fungal strains), belonging to Rhodococcus sp., Pseudomonas sp., Bacillus sp., Streptomyces sp., Aspergillus sp., Cladosporium sp., and Trichoderma sp., formed carbonate precipitates in the range of 89.77~383.37 mg. Moreover, the formation of carbonate precipitates was related to specific metabolisms by which microbes can raise the pH (from 7.20 to >8.00), suggesting that soil microbes that can enhance pH values by specific metabolisms containing the function of carbonate formation. Although an in situ experiment is needed to confirm such capacity, these results showed that soil bacteria and fungi existing in the saline–alkali farmland soil can form carbonate precipitates. The present study provided a microbial perspective for the mechanism of soil inorganic carbon formation, further implying a microbial potential of soil carbon sequestration in saline–alkali farmlands.
Real-Time Measurement of Atmospheric CO2, CH4 and N2O above Rice Fields Based on Laser Heterodyne Radiometers (LHR)
Authors: Li Jun , Xue Zhengyue , Li Yue , Bo Guangyu , Shen Fengjiao , Gao Xiaoming , Zhang Jian , Tan Tu
Published: January 2023

High-precision observations provide an efficient way to calculate greenhouse gas emissions from agricultural fields and their spatial and temporal distributions. Two high-resolution laser heterodyne radiometers (LHRs) were deployed in the suburb of Hefei (31.9°N 117.16°E) for the remote sensing of atmospheric CO2, CH4 and N2O above rice paddy fields. The atmospheric transmittance spectra of CO2, CH4 and N2O were measured simultaneously in real time, and the atmospheric total column abundance was retrieved from the measured data based on the optimal estimation algorithm, with errors of 0.7 ppm, 4 ppb and 2 ppb, respectively. From July to October, the abundance of CO2 in the atmospheric column that was influenced by emissions from rice fields increased by 0.7 ppm CH4 by 30 ppb, and by 4 ppb N2O. During the rice growth season, rice paddy fields play a role in carbon sequestration. CH4 and N2O emissions from paddy fields are negatively correlated. The method of baking rice paddy fields reduces CH4 emissions from rice fields, but N2O emissions from rice fields are usually subsequently increased. The measurement results showed that LHRs are highly accurate in monitoring atmospheric concentrations and have promising applications in monitoring emissions from rice paddy fields. In the observation period, rice paddy fields can sequester carbon, and CH4 and N2O emissions from rice fields are negatively correlated. The LHRs have strong application prospects for monitoring emissions from agricultural fields.
Screening of the Acaricidal Activity of Essential Oils against Panonychus citri (McGregor) (Acari: Tetranychidae)
Authors: Zhu Yijing , Wu Taoqi , Xie Yongjian , Wu Yixuan , Deng Jianyu , Cao Lidong , Zhang Zhijun , Rao Qiong
Published: January 2023

The citrus red mite, Panonychus citri (McGregor), is an important pest of citrus in China, where pesticides are commonly used in citrus orchards. In order to reduce the use of chemical pesticides against P. citri and the development of resistance, the screening of biological control agents has attracted the attention of students. In this study, seven plant essential oils with high toxicity were selected from 40 plant essential oils by the leaf-dip bioassay, including plant essential oils of lemongrass, patchouli, juniper berry, sage, clove, frankincense, and citrus. The LC50 after 72 h treatment were 3.198 μL/mL, 8.312 μL/mL, 3.244 μL/mL, 6.701 μL/mL, 8.350 μL/mL, 21.953 μL/mL, and 8.788 μL/mL, respectively. According to the LT50, the essential oils’ acute toxicity to P. citri from high to low were lemongrass, juniper berry, patchouli, citrus, sage, vetiver, and frankincense essential oils. In general, lemongrass and juniper berry essential oils have the best acaricidal effect and have high application value for the biological control of P. citri, which provides a basis for the development of botanical acaricides.
A Scoping Review of Non-Structural Airway Disease as a Cause of Poor Performance in Racehorses
Authors: Cullinane Ann , Garvey Marie , Walsh Cathal , Gibbons James , Creighton Alan
Published: January 2023

The association between poor performance and respiratory disease in Thoroughbred racehorses that do not have a structural abnormality of the respiratory tract, is often based on anecdotal evidence. The objective of this scoping review was to examine the scientific evidence for such associations. Publications were selected based on a search of three databases (PubMed, Scopus, and CAB Direct), in English and without date restriction, followed by a screening process to exclude non-relevant papers, duplicates, and reviews. This process identified 996 publications of which 20 were analysed using the Quality in Prognosis Studies (QUIPS) tool. The results indicated that the evidence supporting the relationship between proposed diagnostic indicators and poor performance is variable. There is a need for better quality evidence. In particular, there are conflicting reports relating to the impact of equine asthma and EIPH on athletic performance. Furthermore, a lack of standardisation in the measurement of racehorse performance makes it difficult to compare findings from different studies. The industry would benefit from high-level guidance concerning the design of controlled performance studies in Thoroughbred racehorses to collect comprehensive data and facilitate targeted interventions.
Effects of Supplementation with Bee Pollen and Propolis on Growth Performance and Serum Metabolites of Rabbits: A Meta-Analysis
Authors: Sierra-Galicia María Inés , Rodríguez-de Lara Raymundo , Orzuna-Orzuna José Felipe , Lara-Bueno Alejandro , Ramírez-Valverde Rodolfo , Fallas-López Marianela
Published: January 2023

The objective of this study was to evaluate the effects of bee pollen (BP) and propolis (PRO) supplementation on rabbits’ productive performance and serum metabolites through a meta-analysis. Sixteen peer-reviewed publications were included in the data set. The rabbit strains used in the studies included in the data set were New Zealand White, V-line, Rex, and V-line crosses. Weighted mean differences (WMD) between treatments supplemented with BP or PRO and control treatments were used to assess the magnitude of the effect. BP supplementation decreased (p < 0.001) daily feed intake (DFI) and feed conversion ratio (FCR); however, increased (p < 0.001) average daily gain (ADG) and hot carcass yield (HCY). PRO supplementation reduced DFI (p = 0.041) and FCR (p < 0.001), and increased ADG (p < 0.001) and HCY (p = 0.005). In blood serum, BP supplementation increased total antioxidant capacity (TAC; p = 0.002) and decreased serum creatinine concentration (p = 0.049). Likewise, decreased serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA) were detected in response to BP supplementation (p < 0.05). PRO supplementation increased the TAC in blood serum (p = 0.018); however, decreased serum concentrations of AST, ALT, and MDA were observed (p < 0.05). In conclusion, BP or PRO supplementation can be used as a natural growth promoter in rabbits, and both can also improve rabbits' antioxidant status. However, BP or PRO supplementation does not affect rabbits' renal or hepatic health status.
Spatial Learning by Using Non-Visual Geometry and a Visual 3D Landmark in Zebrafish (Danio rerio)
Authors: Baratti Greta , Boffelli Sara , Potrich Davide , Sovrano Valeria Anna
Published: January 2023

Fish conjoin environmental geometry with conspicuous landmarks to reorient towards foraging sites and social stimuli. Zebrafish (Danio rerio) can merge a rectangular opaque arena with a 2D landmark (a blue-colored wall) but cannot merge a rectangular transparent arena with a 3D landmark (a blue cylinder) without training to “feel” the environment thanks to other-than-sight pathways. Thus, their success is linked to tasks differences (spontaneous vs. rewarded). This study explored the reorientation behavior of zebrafish within a rectangular transparent arena, with a blue cylinder outside, proximal to/distal from a target corner position, on the short/long side of the arena. Adult males were extensively trained to distinguish the correct corner from the rotational one, sharing an equivalent metric–sense relationship (short surface left, long surface right), to access food and companions. Results showed that zebrafish’s reorientation behavior was driven by both the non-visual geometry and the visual landmark, partially depending on the landmark’s proximity and surface length. Better accuracy was attained when the landmark was proximal to the target corner. When long-term experience was allowed, zebrafish handled non-visual and visual sensory stimulations over time for reorienting. We advance the possibility that multisensory processes affect fish’s reorientation behavior and spatial learning, providing a link through which to investigate animals’ exploratory strategies to face situations of visual deprivation or impairments.
Dietary Corn Silk (Stigma maydis) Extract Supplementation Modulate Production Performance, Immune Response and Redox Balance in Corticosterone-Induced Oxidative Stress Broilers
Authors: Nassar Farid S. , Alsahlawi Abdulaziz M. , Abdellatif Hasan A. E. , Kamel Nancy N. , Abbas Ahmed O.
Published: January 2023

Immunosuppression is a serious consequence of oxidative stress exposure that negatively affects the productivity and profitability of birds, as well as their well-being. Thus, the present investigation was designed to evaluate the potential of corn silk extract (CSE) supplementation to overcome the negative impacts of oxidative stress induced by corticosterone administration (CORT) in broiler chickens. A total of 280 one day old Cobb 500 male chicks were divided into four groups in 2 × 2 factorial arrangements. The experimental groups included CSE supplementation (0 or 500 mg/kg diet, from 20 to 35 days of age) and CORT administration (0 or 25 mg/kg diet, from 22 to 35 days of age) as independent factors. At the end of week five of age, production performance parameters were measured. The humoral and cell-mediated immune response parameters, redox status, and stress markers were determined. Data revealed deleterious effects of CORT administration on the broilers’ body weight, body weight gain, and feed conversion ratio. Moreover, an exponential increase in stress marker levels, in addition to immunosuppression and redox imbalance, were associated with CORT administration. However, CSE supplementation, with its high total phenols content, partially alleviated the negative impacts of CORT administration, as shown by a significant improvement in immune response parameters and antioxidant activity, as well as a reduction in stress marker levels. Furthermore, CSE supplementation to non-stressed birds even significantly improved total antioxidant activity, total white blood cells (TWBCs) count, T-lymphocyte stimulating index, and wattle thickness. It can be concluded that, under stress conditions in commercial broiler farms, dietary CSE supplementation can strongly be recommended to modulate the negative impacts of stress. Therefore, CSE can be used as an effective immunomodulator and antioxidant agent to increase commercial broiler farm productivity and profitability.
Characterization of Autochthonous Strains from the Cecal Content of Creole Roosters for a Potential Use as Probiotics
Authors: Melara Elvia Guadalupe , Avellaneda Mavir Carolina , Rondón Ana Julia , Rodríguez Marlen , Valdivié Manuel , Martínez Yordan
Published: January 2023

Five strains (CLP2, CLP3, CLP4, CLP5, and CLP6) were isolated from the cecal content of Creole roosters fed without antibiotic growth promoters. Biochemical and morphological tests (negative catalase and oxidase) confirmed the presence of lactic acid bacteria. Additionally, considering the 16s RNA, Lactobacillus vaginalis (CLP2, CLP3, CLP5, and CLP6) and Lactobacillus reuteri (CLP4) were identified. All strains (mainly CLP4 and CLP5) showed variable and significant growth (p < 0.001) at different levels of pH. Likewise, all bacterial cultures were quantified at 42 °C, although only strains CLP4 and CLP5 managed to grow at 30 °C. Additionally, the CLP4, CLP5, and CLP6 strains grew from 0.05 to 0.30% of biliary salts. However, only the CLP4 isolate grew at different concentrations of NaCl (2–10%), and CLP5 grew at 2% NaCl. The CLP4 strain was able to inhibit the in vitro growth of enterobacteria such as Escherichia coli ATCC® 11775TM, Salmonella Typhimurium ATCC® 14028TM, and Clostridium perfringens ATCC® 13124TM. In addition, CLP4 had lower sensitivity in the presence of amoxicillin and tetracycline compared to these pathogenic bacteria. Considering these in vitro results, it is necessary to carry out in vivo studies with the CLP4 strain to test the hypothesis of its probiotic effect in poultry.
Comparative Metabolome Analyses of Ivermectin-Resistant and -Susceptible Strains of Haemonchus contortus
Authors: Tuersong Waresi , Liu Xin , Wang Yifan , Wu Simin , Qin Peixi , Zhu Shengnang , Liu Feng , Wang Chunqun , Hu Min
Published: January 2023

Resistance to anthelmintics such as ivermectin (IVM) is currently a major problem in the treatment of Haemonchus contortus, an important parasitic nematode of small ruminants. Although many advances have been made in understanding the IVM resistance mechanism, its exact mechanism remains unclear for H. contortus. Therefore, understanding the resistance mechanism becomes increasingly important for controlling haemonchosis. Recent research showed that the metabolic state of bacteria influences their susceptibility to antibiotics. However, little information is available on the roles of metabolites and metabolic pathways in IVM resistance of H. contortus. In this study, comparative analyses of the metabolomics of IVM-susceptible and -resistant adult H. contortus worms were carried out to explore the role of H. contortus metabolism in IVM resistance. In total, 705 metabolites belonging to 42 categories were detected, and 86 differential metabolites (17 upregulated and 69 downregulated) were identified in the IVM-resistant strain compared to the susceptible one. A KEGG pathway analysis showed that these 86 differential metabolites were enriched in 42 pathways that mainly included purine metabolism; the biosynthesis of amino acids; glycine, serine, and threonine metabolism; and cysteine and methionine metabolism. These results showed that amino acid metabolism may be mediated by the uptake of IVM and related with IVM resistance in H. contortus. This study contributes to our understanding of the mechanisms of IVM resistance and may provide effective approaches to manage infection by resistant strains of H. contortus.
An Evaluation of Laminarin Additive in the Diets of Juvenile Largemouth Bass (Micropterus salmoides): Growth, Antioxidant Capacity, Immune Response and Intestinal Microbiota
Authors: Wu Youjun , Cheng Yan , Qian Shichao , Zhang Wei , Huang Mengmeng , Yang Shun , Fei Hui
Published: January 2023

A 28 day feeding trial was conducted to investigate the growth performance, immune response and intestinal microbiota of laminarin (LAM) supplemented diets in juvenile largemouth bass (Micropterus salmoides). Four hundred and eighty fish (initial average weight: 0.72 ± 0.04 g) were randomly divided into four groups (40 fish per tank with three replicates in each group) Four diets were prepared with LAM supplementation at the doses of 0 (control), 5 g Kg−1 (LL), 10 g Kg−1 (ML) and 15 g Kg−1 (HL), respectively. No significant difference in the specific growth rate (SGR) and hepatosomatic index (HSI) was observed in fish among the four groups, or in the lipid and ash content of fish flesh. In addition, fish in the LL group exhibited much higher antioxidant capacity (p < 0.05), while the diets with the inclusion of 5 and 10 g Kg−1 LAM remarkably decreased the antioxidant capacity of fish (p > 0.05). Dietary LAM at the dose of 5 g Kg−1 inhibited the transcription of interleukin-1β (il-1β) and tumor necrosis factor-α (tnf-α), while promoting the expression of transforming growth factor-β (tgf-β) in fish intestine. Moreover, the beneficial intestinal bacteria Bacteroide, Comamonas and Mycoplasma abundance significantly increased in fish from the LL group, while the content of opportunistic pathogens Plesiomonas, Aeromonas and Brevinema in fish of the HL group was substantially higher than the control group. Overall, the appropriate dose of supplemented LAM in the diet was 5 g Kg−1, while an excessive supplementation of LAM in the diet led to microbial community instability in largemouth bass.
Epigenetic Alterations in Canine Malignant Lymphoma: Future and Clinical Outcomes
Authors: Montaner-Angoiti Esperanza , Marín-García Pablo Jesús , Llobat Lola
Published: January 2023

Canine malignant lymphoma is a common neoplasia in dogs, and some studies have used dogs as a research model for molecular mechanisms of lymphomas in humans. In two species, chemotherapy is the treatment of choice, but the resistance to conventional anticancer drugs is frequent. The knowledge of molecular mechanisms of development and progression of neoplasia has expanded in recent years, and the underlying epigenetic mechanisms are increasingly well known. These studies open up new ways of discovering therapeutic biomarkers. Histone deacetylases and demethylase inhibitors could be a future treatment for canine lymphoma, and the use of microRNAs as diagnosis and prognosis biomarkers is getting closer. This review summarises the epigenetic mechanisms underlying canine lymphoma and their possible application as treatment and biomarkers, both prognostic and diagnostic.
Non-Lethal Dose-Response Models Replace Lethal Bioassays for Predicting the Hazard of Para-Aminopropiophenone to Australian Wildlife
Authors: Marks Clive A. , Allen Lee , Lindeberg Heli
Published: January 2023

Para-aminopropiophenone (PAPP) is a potent methaemoglobin (MetHb) forming agent used for the lethal control of exotic carnivores and mustelids. To assess the sensitivity of Australian wildlife to PAPP we developed an in vivo assay that did not use death as an endpoint. Sub-lethal dose-response data were modelled to predict PAPP doses required to achieve an endpoint set at 80% MetHb (MetHb80). The comparative sensitivity of non-target mammals referenced to this endpoint was found to be highly variable, with southern brown bandicoots (Isoodon obesulus) the most sensitive species (MetHb80 = 6.3 mg kg−1) and bush rats (Rattus fuscipes) the most tolerant (MetHb80 = 1035 mg kg−1). Published LD50 estimates were highly correlated with PAPP doses modelled to achieve the MetHb80 endpoint (r2 = 0.99, p < 0.001). Most dose-response data for native mammals were collected in the field or in semi-natural enclosures, permitting PAPP and placebo dosed animals to be fitted with tracking transmitters and transponders and released at their point of capture. A protracted morbidity and mortality was observed only in Australian ravens (Corvus coronoides). The combination of sub-lethal dose-response assay and survival data collected in the field provided more relevant information about the actual hazard of pest control agents to non-target wildlife species than laboratory-based lethal-dose bioassays. We discuss the need to replace lethal-dose data with biologically meaningful insights able to define a continuum of toxicological hazards that better serve the needs of conservation and veterinary scientists and wildlife managers.
Disposable Airway Pressure Manometers for Endotracheal Tube Cuff Inflation
Authors: Klonner Moriz Ettore , Mattaliano Giorgio , Casoria Vincenzo , Vogl Claus , Braun Christina
Published: January 2023

This study aimed to assess the performance, accuracy, precision and repeatability of two single-use airway pressure manometers as a cost-effective alternative for inflation of endotracheal tubes with high-volume, low-pressure cuffs. The manometers were tested in a bench top model against a U-tube manometer. Eighteen units of each device were tested. Three consecutive measurements were performed at pressures of 20, 25 and 30 cmH2O each. The mean ± SD of the recorded pressures and maximum deviation from the target pressures were calculated for each device and each target pressure. For device A, the mean ± SD pressures were 19.6 ± 0.7, 23.6 ± 0.8 and 28.3 ± 0.8 cmH2O; for device B, the mean ± SD pressures were 19.3 ± 0.6, 24.3 ± 0.9 and 29.2 ± 0.67 cmH2O for target pressures of 20, 25 and 30 cmH2O, respectively. The bias for device A was −0.4, −1.4, and −1.7 cmH2O and for device B, −0.7, −0.7, and −0.8 cmH2O for target pressures of 20, 25, and 30 cmH2O, respectively. Both devices showed results comparable to those reported for commercial cuff manometers. They represent inexpensive tools that provide clinically sufficient accuracy, precision and repeatability for ETT cuff inflation between pressures of 20 and 30 cmH2O.
Placental Development and Physiological Changes in Pregnant Ewes in Silvopastoral and Open Pasture Systems during the Summer
Authors: Dada Julia Morgana Vieira , Santos Matheus Luquirini Penteado dos , Dani Ana Paula Schneiders , Dammann Cecília Paulina Johann , Pinto Letícia , Vieira Frederico Márcio Corrêa , Barros Flávia Regina Oliveira de
Published: January 2023

This study aimed to analyze the reproductive and physiological changes in ewes subjected to heat stress during pregnancy at UTFPR-Brazil. Twenty-four pregnant crossbred ewes were kept in a silvopastoral system (SP) or an open pasture system (OP) throughout the final trimester of pregnancy. Both systems were stressful, but the SP system had lower air temperature than the OP system (26.0 ± 0.38 and 26.9 ± 0.41 °C, respectively; p = 0.0288). Moreover, the radiant thermal load of the two groups presented a difference of 34 Wm−2 (p = 0.0288), and the grass temperature was also lower in the SP system compared to that in the OP system (23.4 ± 0.37 and 25.6 ± 0.44 °C, respectively; p = 0.0043). The respiratory and heart rates of animals from the OP group were higher than those from the SP group (p < 0.001), but no difference was observed in the mobilization of white blood cells (p = 0.4777), and the neutrophil count was only affected by time (p < 0.0001). As regards placental biometry, placentas in twin pregnancies had a greater membrane area (p = 0.0223), but no differences between the systems were observed in placental weight (p = 0.1522) and the number of cotyledons (p = 0.5457). We concluded that the type of rearing system used affects the thermal comfort of pregnant ewes, and that an SP system can offer more amenable microclimatic conditions, which result in greater comfort for the ewes.
Effects of Perinatal Antibiotic Exposure and Neonatal Gut Microbiota
Authors: Morreale Chiara , Giaroni Cristina , Baj Andreina , Folgori Laura , Barcellini Lucia , Dhami Amraj , Agosti Massimo , Bresesti Ilia
Published: January 2023

Antibiotic therapy is one of the most important strategies to treat bacterial infections. The overuse of antibiotics, especially in the perinatal period, is associated with long-lasting negative consequences such as the spread of antibiotic resistance and alterations in the composition and function of the gut microbiota, both of which negatively affect human health. In this review, we summarize recent evidence about the influence of antibiotic treatment on the neonatal gut microbiota and the subsequent negative effects on the health of the infant. We also analyze the possible microbiome-based approaches for the re-establishment of healthy microbiota in neonates.
Impact of Antibiotic Consumption on Antimicrobial Resistance to Invasive Hospital Pathogens
Authors: Medic Deana , Bozic Cvijan Bojana , Bajcetic Milica
Published: January 2023

The aim of our investigation is to correlate the wholesale data on antibiotic consumption expressed in daily doses per 1000 inhabitants per day (DID) with the resistance rate of invasive pathogen bacteria from 2017 to 2021. The data on antimicrobial resistance were collected from an analysis of the primary isolates of hospitalized patients. According to the CAESAR manual, the selected pathogens isolated from blood culture and cerebrospinal fluids were tested. The consumption of antibiotics for systematic use showed a statistically significant increasing trend (β = 0.982, p = 0.003) from 21.3 DID in 2017 to 34.5 DID in 2021. The ratio of the utilization of broad-spectrum to narrow-spectrum antibiotics increased by 16% (β = 0.530, p = 0.358). The most consumed antibiotic in 2021 was azithromycin (15% of total consumption), followed by levofloxacin (13%) and cefixime (12%). A statistically positive significant correlation was discovered between the percentage of resistant isolates of K. pneumoniae and consumption of meropenem (r = 0.950; p = 0.013), ertapenem (r = 0.929; p = 0.022), ceftriaxone (r = 0.924; p = 0.025) and levofloxacin (r = 0.983; p = 0.003). Additionally, the percentage of resistant isolates of E. coli and consumption of ertapenem showed significant correlation (r = 0.955; p = 0.011). Significant correlation with consumption of the antibiotics widely used at the community level, such as levofloxacin, and resistance isolated in hospitals indicates that hospital stewardship is unlikely to be effective without a reduction in antibiotic misuse at the community level.
Therapeutic Options and Outcomes for the Treatment of Children with Gram-Positive Bacteria with Resistances of Concern: A Systematic Review
Authors: Chiusaroli Lorenzo , Liberati Cecilia , Rulli Luigi , Barbieri Elisa , De Pieri Marica , Di Chiara Costanza , Mengato Daniele , Giaquinto Carlo , Donà Daniele
Published: January 2023

Background: Methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant coagulase-negative Staphylococci (MR-CoNS), and vancomycin-resistant Enterococci (VRE) are increasing worldwide and represent a threat for the limited treatment options in pediatric patients and neonates compared to adults. Recommendations in pediatrics are mainly extrapolated from adults’ studies. Methods: A literature search for the treatment of these pathogens in children (<18 years old) was conducted in Embase, MEDLINE, and Cochrane Library. Studies reporting data on single-patient-level outcomes related to a specific antibiotic treatment for multidrug resistant (MDR) Gram-positive bacterial infection in children were included. Studies reporting data from adults and children were included if single-pediatric-level information could be identified (PROSPERO registration: CRD42022383867). Results: The search identified 11,740 studies (since January 2000), of which 48 fulfilled both the inclusion and the exclusion criteria and were included in the analysis: 29 for MRSA, 20 for VRE, and seven for MR-CoNS. Most studies were retrospective studies. Vancomycin was mainly used as a comparator, while linezolid and daptomycin were the most studied antimicrobials showing good efficacy. Conclusions: Linezolid showed a safety and efficacy profile in a neonatal setting; daptomycin is increasingly used for MRSA, but the evidence is scarce for VRE.