bluuebunny/crossref_metadata_2025 huggingface dataset:
The Dilemma of Misclassification Rates in Senior Patients With Prostate Cancer, Who Were Treated With Robot-Assisted Radical Prostatectomy: Implications for Patient Counseling and Diagnostics
Authors: Liakos Nikolaos , Witt Joern H. , Rachubinski Pawel , Leyh-Bannurah Sami-Ramzi
Published: February 2022

Objectives

There is a recent paradigm shift to extend robot-assisted radical prostatectomy (RARP) to very senior prostate cancer (PCa) patients based on biological fitness, comorbidities, and clinical PCa assessment that approximates the true risk of progression. Thus, we aimed to assess misclassification rates between clinical vs. pathological PCa burden.

Materials and Methods

We compared senior patients with PCa ≥75 y (n = 847), who were propensity score matched with younger patients <75 y (n = 3,388) in a 1:4 ratio. Matching was based on the number of biopsy cores, prostate volume, and preoperative Cancer of the Prostate Risk Assessment (CAPRA) risk groups score. Multivariable logistic regression models (LRMs) predicted surgical CAPRA (CAPRA-S) upgrade, which was defined as a higher risk of the CAPRA-S in the presence of lower-risk preoperative CAPRA score. LRM incorporated the same variables as propensity score matching. Moreover, patients were categorized as low-, intermediate-, and high-risk, preoperative and according to their CAPRA and CAPRA-S scores.

Results

Surgical CAPRA risk strata significantly differed between the groups. Greater proportions of unfavorable intermediate risk (39 vs. 32%) or high risk (30 vs. 28%; p < 0.001) were observed. These proportions are driven by greater proportions of International Society of Urological Pathology (ISUP) Gleason Grade Group 4 or 5 (33 vs. 26%; p = 0.001) and pathological tumor stage (≥T3a 54 vs. 45%; p < 0.001). Increasing age was identified as an independent predictor of CAPRA-S-based upgrade (age odds ratio [OR] 1.028 95% CI 1.02–1.037; p < 0.001).

Conclusion

Approximately every second senior patient has a misclassification in (i.e., any up or downgrade) and each 4.5th senior patient specifically has an upgrade in his final pathology that directly translates to an unfavorable PCa prognosis. It is imperative to take such substantial misclassification rates into account for this sensitive PCa demographic of senior men. Future prospective studies are warranted to further optimize PCa workflow and diagnostics, such as to incorporate modern imaging, molecular profiling and implement these into biopsy strategies to identify true PCa burden.
New Insights into the Use of Rhizobia to Mitigate Soil N2O Emissions
Authors: Hénault Catherine , Barbier Elodie , Hartmann Alain , Revellin Cécile
Published: February 2022

Agriculture is a major anthropogenic source of the greenhouse gas N2O, which is also involved in stratospheric ozone depletion. While the use of rhizobial inoculants has already been reported as an emerging option for mitigating soil N2O emissions, this study presents an in situ abatement of 70% of soil N2O emission using the strain nosZ+ G49 vs. nosZ− USDA138 in association with soybean. Therefore, we consider that the choice of the inoculant strain of a leguminous crop should take into account the capacity of strains to reduce nitrous oxide in addition to their N fixation capacity. This study also clearly suggests that this mitigation option could be considered not only for soybean but also for different leguminous crops, with emphasis currently placed on lupin because of the potential of its association with the nosZ+ LL200 strain. The clear demonstration of the N2O reduction capacity of clover symbiotic strains suggests that opportunities for mitigation might also occur on grassland.
Yield and Quality of Three Cultivars of Dark Fire-Cured (Kentucky) Tobacco (Nicotiana tabacum L.) Subjected to Organic (Compost) and Mineral Nitrogen Fertilization
Authors: Sifola Maria Isabella , Cozzolino Eugenio , Di Mola Ida , Ottaiano Lucia , del Piano Luisa , Mori Mauro
Published: February 2022

A biennial experiment (2009 and 2010) was conducted at Calvi (Benevento, Southern Italy) to evaluate the effect of compost by organic fraction municipal solid waste (OFMSW), in combination with mineral nitrogen (N) fertilization, on yield and quality of three Dark Fire-cured (Kentucky) tobacco cultivars commonly cultivated at Benevento province (Campania region, Southern Italy). Six N fertilization treatments (N0 = soil N reserves available for plant growth; MIN = 135 kg ha−1 of N applied as mineral fertilizer; C10 = 10 Mg d.w. ha−1 compost; C10N = 10 Mg d.w. ha−1 compost + 50% MIN; C20 = 20 Mg d.w. ha−1 compost; C20N = 20 Mg d.w. ha−1 compost + 50% MIN) were combined with the following cultivars: (i) Foiano, medium early maturing; (ii) Riccio Beneventano (local ecotype), medium maturing; (iii) SKL, medium maturing. Yield of cured leaves (Mg ha−1) and growth components (number of leaves per plant, mean individual leaf area, leaf area per plant, specific leaf weight, stem diameter and height) and color parameters (L*, a*/b*) were measured. Leaf quality traits (nitrates, total N and alkaloids contents, score) and N use efficiency were also determined. The best growth and yield performance was reached in 2010 when plants were taller, developed both stems that were more robust and leaves having greater individual leaf area, and showed a higher leaf area per plant than in the first year. Regardless of the form of applied N (compost, mineral fertilizer, or a combination of both), tobacco plants appeared to be directly and positively influenced by increasing quota of readily available N received by each treatment, which was determined at the beginning of field growth by N soil balance and taking into account the percentage of N supplied by organic (compost) and mineral fertilizers. Results obtained with compost treatments, particularly when combined with mineral fertilizer (at C10N more than C20N), appeared comparable or sometimes better than those of full mineral fertilization although N fertilization by synthetic products was applied at very low doses.
Animal Welfare and the Acknowledgment of Cultural Differences
Authors: Garcia Arlene , McGlone John J.
Published: February 2022

Pigs are considered sentient beings that have a mental capability that warrants attention to their welfare. Cultural values towards animal welfare differ in world regions. Still, authors have argued for worldwide harmonization of animal welfare rules. At the same time, the focus of many animal welfare rules do not address the most significant problems on modern commercial pig farms. The foci of animal welfare rules are often on space (quantity and quality), acute painful practices, equipment, and caretaker behavior. However, most serious animal welfare issues are related to episodic events such as compromised pigs, lack of appropriately skilled staff, and human behavior (or lack thereof) towards animals. Modern technologies such as image, sound, and building oversight by automated systems can potentially provide better individual pig care. The future should bring us solutions to identify and resolve episodic negative animal welfare events. The other issues of space and painful practices are best improved by using science-based solutions. We propose that science be the key resource to making animal welfare decisions, but with a healthy appreciation and respect for cultural differences in our views of animals and the economic impact of rules. Colonialism is not viewed positively today, and economic neo-colonialism should not be allowed to replace it. Respect for cultural differences should play a role in animal welfare rules within and among countries.
Morphological Characteristics of a Horse Discovered in an Avar-Period Grave from Sâncraiu de Mureș (Alba County, Romania)
Authors: Gudea Alexandru , Martonos Cristian , Cosma Călin , Stan Florin
Published: February 2022

Archeozoological studies provide an insight into human–environment relations, bringing important information on the morphology of the animal and the role of the animal and its functions. The purpose of this study was to reveal the morphological characteristics of the horse identified in an 8th century BC Avar cemetery dated, as it resulted from the investigation carried out on the materials presented to the Anatomy Lab of Department of Comparative Anatomy. The cleaning and conditioning of the materials were performed in the lab, followed by anatomical and osteometrical study. The identification of the species, the osteometrical interpretation and the assessment of age at death constituted the basis of the main conclusions. Based on the morphological and metrical data, we concluded that the fragments originated from a single young horse individual (Equus caballus) no older than 2.5 (2–2.5 years age span) included into class 5 of height (Vitt scale) with slender extremities. An overview of the available data (horse morphology) from similar sites in Romania and neighboring territories (Hungary and Croatia) is presented, with the intention of a general framing for the characteristics of the horses used by the Avar populations.
Livestock–Carnivore Coexistence: Moving beyond Preventive Killing
Authors: Chinchilla Skarleth , Berghe Eric van den , Polisar John , Arévalo Constanza , Bonacic Cristian
Published: February 2022

Livestock predation is a global problem and constitutes the main source of conflict between large carnivores and human interests. In Latin America, both jaguar and puma are known to prey on livestock, yet studies in Mesoamerica have been scattered and few have been carried out in Honduras. We interviewed ranchers in a biosphere reserve where jaguars and pumas are present. Local indigenous communities reported livestock predation (average annual loss of 7% from 2010–2019), with preventive and retaliatory killing as their main actions against predation by the jaguar and puma. Other sources of cattle loss included diseases and theft. The extensive management system (free grazing) lets cattle access forests where predators are more common. We found that livestock predation is not random, but rather, related to landscape variables and human influence. Sites farther from human influence and closer to forest cover were more susceptible to predation. Jaguar and puma persistence in the biosphere reserve will require measures that facilitate human–carnivore coexistence and comply with Sustainable Development Goals (SDG) 2 and 15 (zero hunger and biodiversity conservation). We propose management practices to mitigate livestock predation in the presence of large carnivores based on examples of proven human–carnivore coexistence in Venezuela, Brazil, Paraguay, and Nicaragua, such as improving the spatial arrangement of livestock (maintaining a distance from forest areas) and the incorporation of confinement pens for young calves (at least the first three months of life) and their mothers. If the pens are built close to the property’s house and have constant surveillance and/or dogs, the results are likely to be more effective. Deploying these proven tools may help change the current negative perception of ranchers towards large carnivores that is essential to conservation under the aims of SDG 15. We recommend government policies and support aimed to strengthen livestock health to increase productivity and to reduce their vulnerability to predation. Finally, this study represents a baseline to understand the magnitude of the human–carnivore conflict over cattle in one of the largest biosphere reserves in Mesoamerica.
The Association between Blood Β-Hydroxybutyric Acid Concentration in the Second Week of Lactation and Reproduction Performance of Lithuanian Black and White Cows
Authors: Mečionytė Indrė , Palubinskas Giedrius , Anskienė Lina , Antanaitis Ramūnas , Yilmaz Ayhan , Tapio Ilma , Žilaitis Vytuolis
Published: February 2022

Hyperketonemia is a very common metabolic state in dairy cows, which result in lower milk production, impaired fertility, and increased frequency of other diseases. In this study, we aimed to determine the influence of season, parity, and milk yield of cows on beta-hydroxybutyrate (BHB) concentration in the second week of lactation (WK 2) and establish the relationship between BHB concentration in WK 2 and reproduction performance traits such as insemination rate and first insemination day of Lithuanian Black and White dairy cows. The study included clinically healthy Lithuanian Black and White cows (n = 692). Blood BHB concentration was measured using capillary blood samples collected after morning milking when cows were 7–10 DIM. The impact of WK 2 blood BHB concentration on the insemination rate and first insemination day were investigated. The effect of BHB was evaluated according to the season, parity, and milk yield per lactation (305 DIM). Significant differences were observed in BHB concentration in WK 2 due to season and parity, but no statistically significant differences were observed for milk yields (305 d). Increased blood BHB concentration in WK 2 negatively affected insemination rate (p < 0.001) and first insemination day (p < 0.001). The study findings indicate that BHB concentration in WK 2 depends on season and parity, while the milk yield is not associated with BHB concentration. High BHB concentration in WK 2 increases insemination rate and delays the first insemination day for high milk-yielding Lithuanian Black and White dairy cows.
BcGR1.1, a Cytoplasmic Localized Glutathione Reductase, Enhanced Tolerance to Copper Stress in Arabidopsis thaliana
Authors: Li Yan , Huang Feiyi , Tao Yu , Zhou Ying , Bai Aimei , Yu Zhanghong , Xiao Dong , Zhang Changwei , Liu Tongkun , Hou Xilin , Li Ying
Published: February 2022

Copper is a mineral element, which is necessary for the normal growth and development of plants, but high levels of copper will seriously damage plants. Studies have shown that AtGR1 improves the tolerance of Arabidopsis to aluminum and cadmium stress. However, the role of GR in the copper stress response of plants is still unclear. Here, we identified four genes (named BcGR1.1, BcGR1.2, BcGR2.1 and BcGR2.2, respectively) encoding glutathione reductase (GR) in non-heading Chinese cabbage (Brassica campestris (syn. Brassica rapa) ssp. chinensis), which could be divided into two types based on the subcellular localization. Among them, BcGR1.1, which belonged to the cytoplasmic localization type, was significantly upregulated under copper stress. Compared to WT (the wild type), Arabidopsis thaliana heterologously overexpressed BcGR1.1 had longer roots, higher fresh weight, higher GSH levels and GSH/GSSG (oxidized form of GSH) ratio, and accumulated more superoxide dismutase and peroxidase under copper stress. However, in the AsA-GSH cycle under copper stress, the contents of AsA and AsA/DHA were significantly downregulated, and the contents of DHA and T-AsA (total AsA) were upregulated, in the BcGR1.1-overexpressing Arabidopsis. Therefore, BcGR1.1 could improve the scavenging ability of reactive oxygen species (ROS) by increasing the activity of GR, antioxidant enzymes and the utilization of AsA, and then enhance the copper stress tolerance of plants.
Effect of Wind Loads on Collapse Performance and Seismic Loss for Steel Ordinary Moment Frames
Authors: Kim Taeo , Han Sang Whan , Cho Soo Ik
Published: February 2022

The aim of this study is to investigate the effect of wind loads on the seismic collapse performance and seismic loss for steel ordinary moment frames (OMFs). For this purpose, 9-, 12-, 15-, and 18-story steel OMFs are repeatedly designed for (1) gravity load + seismic load, (2) gravity load + seismic load + wind load (wind speed = 44 m/s), and (3) gravity load + seismic load + wind load (wind speed = 55 m/s). The seismic collapse performance and seismic loss of OMFs are evaluated using the procedures in FEMA P695 (FEMA, 2009) and FEMA P58 (FEMA, 2018), respectively. Steel OMFs designed with consideration of wind loads have larger member sections than corresponding steel OMFs designed without consideration of wind loads as expected. Although member sections are increased when wind loads are considered, the growth in the maximum base shear force and lateral stiffness of OMFs are insignificant. Unlike our expectation, OMFs designed with consideration of wind loads have higher expected annual loss (EAL) than corresponding OMFs designed without consideration of wind loads.
CRCSI: A Generic Block Interleaver for the Next Generation Terrestrial Broadcast Systems
Authors: Li Ruijia , Tian Jinfeng , Bian Xin , Li Mingqi
Published: February 2022

In order to combat the impulse noise and channel time-frequency selective fading, it is essential to use channel interleavers. Among them, the block interleaver has been widely used in terrestrial broadcasting systems such as DVB-T2 and ATSC 3.0, since it can better reflect the mapping relationship of data units on time-frequency resources. Due to the limited value of the interleaving structure parameter, the existing block interleavers cannot fully exploit time and frequency diversities, which leads to a loss of partial diversity gains. To address this problem, this paper proposes a generic block interleaver for channel interleaving through jointly considering the time and frequency diversities. First, row crossing is performed according to a certain interval, which will increase the interleaving distance; then, cyclic shift is used to achieve a greater time diversity gain. The proposed method can achieve flexible parameter configuration, which can effectively increase the scalability of the parameters and make the interleaver more versatile. Theoretical analysis shows that a larger interleaving distance is obtained and the multiplicity of minimum distance is significantly reduced. The simulation results of the fixed and mobile reception scenarios in the terrestrial broadcasting systems demonstrate the effectiveness of proposed interleaver compared to existing block interleavers.
Quantitative Effects of Climate Change on Vegetation Dynamics in Alpine Grassland of Qinghai-Tibet Plateau in a County
Authors: Liu Hui , Song Xiaoyu , Wen Wang , Jia Qiong , Zhu Deming
Published: February 2022

Alpine grassland in the Qinghai-Tibet Plateau is known to be sensitive to climate change. To quantify the impacts of climate change on alpine ecosystems at small scale, Wulan County in Qinghai Province was taken as the research object, and the relationships between vegetation dynamics and climate changes and the direct and indirect effects of climate factors on vegetation dynamics were analyzed using the methods of ordinary least squares, Pearson correlation analysis and path analysis, on the basis of MOD13A3 data and meteorological data from 2001 to 2020. The results showed that the Normalized Difference Vegetation Index (NDVI) in the growing season of the county and 5 vegetation types showed similar fluctuation processes and relationships with climate factors during the study period. The growing season NDVI (GSN) of shrubland and desert steppe respectively were the highest and lowest. The yearly mean values of GSN over the county ranged from 0.151 to 0.264, and increased extremely significantly with years at a rate of 0.0035 yr−1. Spatially, GSN gradually decreased from northeast to southwest, and 97.2% of the county area showed an increasing trend in GSN. With years, growing season evaporation (GSE) decreased extremely significantly at a rate of 29.6 mm yr−1, while growing season average relative humidity (GSARH) showed a significant increasing trend at a rate of 0.16% yr−1. The correlations and effects of GSE, GSARH, and growing season precipitation (GSP) on vegetation dynamics were weakened in turn. GSE was the main direct effect factor, and the latter two were the indirect effect factors through GSE. The total contribution rates of GSE, GSARH and GSP to vegetation dynamics was about 78.0%. However, growing season average temperature (GSAT) had little effect on vegetation dynamics. This study provides information for understanding the characteristics of vegetation dynamics of alpine grassland in the Qinghai-Tibet Plateau at a small scale.
Chromatic Bacteria v.2-A Himar1 Transposon-Based Delivery Vector to Extend the Host Range of a Toolbox to Fluorescently Tag Bacteria
Authors: Stocks Christian , Schlechter Rudolf O. , Remus-Emsermann Mitja N. P.
Published: February 2022

A recent publication described the construction and utility of a comprehensive “Chromatic Bacteria” toolbox containing a set of genetic tools that allows for fluorescently tagging a variety of Proteobacteria. In an effort to expand the range of bacteria taggable with the Chromatic Bacteria toolbox, a series of Himar1 transposon vectors was constructed to mediate insertion of fluorescent protein and antibiotic resistant genes. The Himar1 transposon was chosen as it is known to function in a wide range of bacterial species. To test the suitability of the new Himar1 Chromatic Bacteria plasmid derivatives, conjugations were attempted on recently isolated non-model organisms. Although we were unsuccessful in delivering the plasmids into Gram-positive bacterial isolates, we successfully modified previously recalcitrant isolates to the first set of the Chromatic Bacteria toolbox, such as Sphingomonas sp. Leaf357 and Acidovorax sp. Leaf84. This manuscript reports on the currently available plasmids and transposition success in different bacteria.
The Effect of Daylight Illumination in Nursing Buildings on Reading Comfort of Elderly Persons
Authors: Fu Yao , Wu Yue , Gao Weijun , Hui Rong
Published: February 2022

Reading is one of the popular activities among elderly persons. A reasonable level of daylight illumination can ensure the visual comfort of reading for elderly persons. State arousal level and subjective comfort report are important parameters reflecting the effect of daylight illumination on visual comfort of reading in elderly persons. In this study, daylight illumination measurements were conducted in a nursing institution of Shenyang, China. Moreover, the methods of electrodermal activity (EDA) physiological index measurement and questionnaire scoring were used to compare and analyze the state arousal level and visual comfort of elderly persons under different illumination conditions. The results show that when elderly persons were involved in their daily reading activity, the acceptable daylight illumination range was between 300 and 1000 lx. When the daylight illumination was between 600 and 800 lx, the state arousal level and visual comfort was high; when it was 700 lx, the state arousal level and visual comfort was the highest. Although 500 and 900 lx both indicated neutral illumination, the evaluations were more consistent at 500 lx than at 900 lx. At 300, 400, and 1000 lx, visual comfort was poor and the state arousal level was low. At 300 lx, visual comfort was the worst and the state arousal level was the lowest. This study provides a reliable reference for architects to design the daylight conditions of the living spaces of the elderly.
Optimizing Shading and Thermal Performances of Vertical Green Wall on Buildings in a Hot Arid Region
Authors: Freewan Ahmed A. , Jaradat Neda’a M. , Amaireh Ikrima A.
Published: February 2022

Due to global concerns about energy issues, global warming, and urban quality, vertical greening systems (VGS) are receiving more attention in construction and design research. Therefore, VGS has become part of building envelope design as a passive technique for saving energy in building sectors. The current study aimed to investigate shading and energy performances of VGS in buildings in hot climate regions and to optimize VGS design as a building design element. The study was conducted through simulation and field experiments in a student housing building at a university campus (Irbid, Jordan). Field measurements were taken to assess the thermal effect of the green wall and daylight performance as well as the efficiency of the typical green wall design configuration. Furthermore, a methodology for accurately representing green walls was established and used. Both simulation and experimentation demonstrated that the thickness of the air cavity and the percentage of foliage coverage can have a substantial impact on the performance of the green wall system. Results showed that green wall systems are effective natural sunscreens and shading systems. A green wall helped to reduce the exterior wall surface temperatures by a range of 6 to 11 °C compared to the base case of the wall without a VGS on different days. In addition, it decreased the interior surface temperature of the investigated southern façade by an average of 5 °C compared to the base case. Green wall design configurations for hot climate regions, such as Jordan, will help designers to use the VGS as a design element. Our findings indicate that GW could help to improve the thermal and daylight environment and thus the results could be taken as indicative for GW wall design in other areas or buildings.
Risk of Second Primary Thyroid Cancer in Women with Breast Cancer
Authors: Cieszyńska Monika , Kluźniak Wojciech , Wokołorczyk Dominika , Cybulski Cezary , Huzarski Tomasz , Gronwald Jacek , Falco Michał , Dębniak Tadeusz , Jakubowska Anna , Derkacz Róża , Marciniak Wojciech , Lener Marcin , Woronko Karolina , Mocarz Dominika , Baszuk Piotr , Bryśkiewicz Marta , Narod Steven A. , Lubiński Jan
Published: February 2022

The goal of this study was to estimate the risk of thyroid cancer following breast cancer and to identify therapeutic and genetic risk factors for the development of thyroid cancer after breast cancer. We followed 10,832 breast cancer patients for a mean of 14 years for new cases of thyroid cancer. All women were genotyped for three Polish founder mutations in BRCA1 (C61G, 4153delA, 5382insC) and four mutations in CHEK2 (1100delC, IVS2 + 1G/A, del5395, I157T). Information was collected on chemotherapy, radiotherapy, hormonal therapies, and oophorectomy. Of the 10,832 women, 53 (0.49%) developed a second primary thyroid cancer. Based on Polish population statistics, the expected number was 12.4 (SIR = 4.3). The ten-year risk of developing thyroid cancer was higher in women who carried a CHEK2 mutation (1.5%) than in women who carried no mutation (0.9%). The age-adjusted hazard ratio for developing thyroid cancer was 1.89 (0.46–7.79; p = 0.38) for those with a CHEK2 protein-truncating mutation and 2.75 (1.29–5.85; p = 0.009) for those with a CHEK2 missense mutation.
Patient-Derived Ovarian Cancer Spheroids Rely on PI3K-AKT Signaling Addiction for Cancer Stemness and Chemoresistance
Authors: Parashar Deepak , Geethadevi Anjali , Mittal Sonam , McAlarnen Lindsey , George Jasmine , Kadamberi Ishaque , Gupta Prachi , Uyar Denise , Hopp Elizabeth , Drendel Holli , Bishop Erin , Bradley William , Bone Kathleen , Rader Janet , Pradeep Sunila , Chaluvally-Raghavan Pradeep
Published: February 2022

Ovarian cancer is the most lethal gynecological malignancy among women worldwide and is characterized by aggressiveness, cancer stemness, and frequent relapse due to resistance to platinum-based therapy. Ovarian cancer cells metastasize through ascites fluid as 3D spheroids which are more resistant to apoptosis and chemotherapeutic agents. However, the precise mechanism as an oncogenic addiction that makes 3D spheroids resistant to apoptosis and chemotherapeutic agents is not understood. To study the signaling addiction mechanism that occurs during cancer progression in patients, we developed an endometrioid subtype ovarian cancer cell line named ‘MCW-OV-SL-3’ from the ovary of a 70-year-old patient with stage 1A endometrioid adenocarcinoma of the ovary. We found that the cell line MCW-OV-SL-3 exhibits interstitial duplication of 1q (q21–q42), where this duplication resulted in high expression of the PIK3C2B gene and aberrant activation of PI3K-AKT-ERK signaling. Using short tandem repeat (STR) analysis, we demonstrated that the cell line exhibits a unique genetic identity compared to existing ovarian cancer cell lines. Notably, the MCW-OV-SL-3 cell line was able to form 3D spheroids spontaneously, which is an inherent property of tumor cells when plated on cell culture dishes. Importantly, the tumor spheroids derived from the MCW-OV-SL-3 cell line expressed high levels of c-Kit, PROM1, ZEB1, SNAI, VIM, and Twist1 compared to 2D monolayer cells. We also observed that the hyperactivation of ERK and PI3K/AKT signaling in these cancer cells resulted in resistance to cisplatin. In summary, the MCW-OV-SL3 endometrioid cell line is an excellent model to study the mechanism of cancer stemness and chemoresistance in endometrioid ovarian cancer.
Modeling the Role of Cancer-Associated Fibroblasts in Tumor Cell Invasion
Authors: Poon Stephanie , Ailles Laurie E.
Published: February 2022

The major cause of cancer-related deaths can be attributed to the metastatic spread of tumor cells—a dynamic and complex multi-step process beginning with tumor cells acquiring an invasive phenotype to allow them to travel through the blood and lymphatic vessels to ultimately seed at a secondary site. Over the years, various in vitro models have been used to characterize specific steps in the cascade to collectively begin providing a clearer picture of the puzzle of metastasis. With the discovery of the TME’s supporting role in activating tumor cell invasion and metastasis, these models have evolved in parallel to accommodate features of the TME and to observe its interactions with tumor cells. In particular, CAFs that reside in reactive tumor stroma have been shown to play a substantial pro-invasive role through their matrix-modifying functions; accordingly, this warranted further investigation with the development and use of invasion assays that could include these stromal cells. This review explores the growing toolbox of assays used to study tumor cell invasion, from the simple beginnings of a tumor cell and extracellular matrix set-up to the advent of models that aim to more closely recapitulate the interplay between tumor cells, CAFs and the extracellular matrix. These models will prove to be invaluable tools to help tease out the intricacies of tumor cell invasion.
Prospect of Prostate Cancer Treatment: Armed CAR-T or Combination Therapy
Authors: Jiang Yao , Wen Weihong , Yang Fa , Han Donghui , Zhang Wuhe , Qin Weijun
Published: February 2022

The incidence rate of prostate cancer is higher in male cancers. With a hidden initiation of disease and long duration, prostate cancer seriously affects men’s physical and mental health. Prostate cancer is initially androgen-dependent, and endocrine therapy can achieve good results. However, after 18–24 months of endocrine therapy, most patients eventually develop castration-resistant prostate cancer (CRPC), which becomes metastatic castration resistant prostate cancer (mCRPC) that is difficult to treat. Chimeric Antigen Receptor T cell (CAR-T) therapy is an emerging immune cell therapy that brings hope to cancer patients. CAR-T has shown considerable advantages in the treatment of hematologic tumors. However, there are still obstacles to CAR-T treatment of solid tumors because the physical barrier and the tumor microenvironment inhibit the function of CAR-T cells. In this article, we review the progress of CAR-T therapy in the treatment of prostate cancer and discuss the prospects and challenges of armed CAR-T and combined treatment strategies. At present, there are still many obstacles in the treatment of prostate cancer with CAR-T, but when these obstacles are solved, CAR-T cells can become a favorable weapon for the treatment of prostate cancer.
Roles of Tumor Markers in Central Nervous System Germ Cell Tumors Revisited with Histopathology-Proven Cases in a Large International Cohort
Authors: Takami Hirokazu , Graffeo Christopher S. , Perry Avital , Giannini Caterina , Nakazato Yoichi , Saito Nobuhito , Matsutani Masao , Nishikawa Ryo , Ichimura Koichi , Daniels David J.
Published: February 2022

The central nervous system germ cell tumor (CNS GCT) is a rare and incompletely understood disease. A major outstanding question in the 2015 consensus document for CNS GCT management was the utility and interpretation of the tumor markers human chorionic gonadotropin (HCG) and alpha fetoprotein (AFP) in the diagnosis of malignant non-germinomatous GCTs (hereafter NGGCTs) prior to treatment. In the current study, we assembled two geographically and ethnically different clinical cohorts from the Mayo Clinic (1988–2017) and the intracranial GCT Genome Analysis Consortium (iGCT Consortium) in Japan to address this question. Patients with both histopathological diagnosis and tumor markers available were eligible for inclusion (n = 162). Biopsy and surgical resection were performed in 85 and 77 cases, respectively. Among 77 resections, 35 demonstrated positivity for HCG, AFP, or both (45%). Seventeen of the marker-positive cases had no malignant non-germinomatous component identified on histopathology, but they were composed strictly of germinoma, teratoma, or both (49%). One embryonal carcinoma was the only marker-negative NGGCT in the study sample. Among 85 biopsies, 18 were marker positive (21%). Seven of these patients had no malignant non-germinomatous component on histopathology, suggesting the potential limitations of limited tissue sample volumes. Neither histopathological diagnosis nor tumor markers alone reliably diagnose NGGCTs due to the secretion of HCG and AFP by germinomas and teratomas. Treatment planning should incorporate integrated histopathological and laboratory-based diagnosis to optimize diagnostic and treatment strategies for this unusual and histologically heterogeneous tumor.
Proximity Ligation Mapping of Microcephaly Associated SMPD4 Shows Association with Components of the Nuclear Pore Membrane
Authors: Piët Alexandra C. A. , Post Marco , Dekkers Dick , Demmers Jeroen A. A. , Fornerod Maarten
Published: February 2022

SMPD4 is a neutral sphingomyelinase implicated in a specific type of congenital microcephaly. Although not intensively studied, SMPD4 deficiency has also been found to cause cell division defects. This suggests a role for SMPD4 in cell-cycle and differentiation. In order to explore this role, we used proximity ligation to identify the partners of SMPD4 in vivo in HEK293T cells. We found that these partners localize near the endoplasmic reticulum (ER) and the nuclear membrane. Using mass spectrometry, we could identify these partners and discovered that SMPD4 is closely associated with several nucleoporins, including NUP35, a nucleoporin directly involved in pore membrane curvature and pore insertion. This suggests that SMPD4 may play a role in this process.
Knowledge, Attitudes, and Practices toward First Aid Management of Skin Burns in Saudi Arabia
Authors: Al Dhafiri Mahdi , Kaliyadan Feroze , Alghadeer Mohammed A. , AL-Jaziri Zainab Y. , Alabdulmuhsin Zainab A. , Alaithan Zainab A.
Published: February 2022

Background: Burns are considered a serious health affection that leads to several consequences affecting a person both physically and emotionally. Herbal and traditional medicine have become popular remedies among patients worldwide. Aim: This study explores common practices followed in burns as first aid management. Methods: A cross-sectional study was conducted in the Eastern province of Saudi Arabia using a designed questionnaire distributed electronically through different social media. The questionnaire consisted of socio-demographic details, history of burns, causative material, and practices followed in response to burns. Results: 461 individuals have participated in this study. The commonest reason for burns was hot water or steam. The majority of the respondents (63%) had a satisfactory response to self-aid alone at home. The most common first aid options for managing burns at home were cold water alone 195 (42%), any sort of cream alone 177 (38%), or both 317 (69%). Overall, the result shows no statistically significant difference between the outcome of burn injury and the most commonly used burn aids. Conclusions: Most people use creams and water as the first-aid management of burns, while a good number of people use traditional medicine. Overall, people who receive hospital treatment after getting first aid at home give a better outcome.
Phase Control Growth of InAs Nanowires by Using Bi Surfactant
Authors: Saleem Samra , Maryam Ammara , Fatima Kaneez , Noor Hadia , Javed Fatima , Asghar Muhammad
Published: February 2022

To realize practical applications of nanowire-based devices, it is critical, yet challenging, to control crystal structure growth of III-V semiconductor nanowires. Here, we demonstrate that controlled wurtzite and zincblende phases of InAs nanowires can be fabricated using bismuth (Bi) as a surfactant. For this purpose, catalyst free selective area epitaxial growth of InAs nanowires was performed using molecular beam epitaxy (MBE). During the growth, Bi was used which may act as a wetting agent influencing the surface energy at growth plane ends, promoting wurtzite crystal phase growth. For a demonstration, wurtzite and zincblende InAs nanowires were obtained with and without using Bi-flux. Photoluminescence spectroscopy (PL) analysis of the nanowires indicates a strong correlation between wurtzite phase and the Bi-flux. It is observed that the bandgap energy of wurtzite and zincblende nanowires are ∼0.50 eV and ∼0.42 eV, respectively, and agree well with theoretical estimated bandgap of corresponding InAs crystal phases. A blue shift in PL emission peak energy was found with decreasing nanowire diameter. The controlled wurtzite and zincblende crystal phase and its associated heterostructure growth of InAs nanowires on Si may open up new opportunities in bandgap engineering and related device applications integrated on Si. Furthermore, this work also illustrates that Bi as a surfactant could play a dynamic role in the growth mechanism of III-V compound semiconductors.
A Review of Recent Developments in Preparation Methods for Large-Area Perovskite Solar Cells
Authors: Hamukwaya Shindume Lomboleni , Hao Huiying , Zhao Zengying , Dong Jingjing , Zhong Tingting , Xing Jie , Hao Liu , Mashingaidze Melvin Mununuri
Published: February 2022

The recent rapid development in perovskite solar cells (PSCs) has led to significant research interest due to their notable photovoltaic performance, currently exceeding 25% power conversion efficiency for small-area PSCs. The materials used to fabricate PSCs dominate the current photovoltaic market, especially with the rapid increase in efficiency and performance. The present work reviews recent developments in PSCs’ preparation and fabrication methods, the associated advantages and disadvantages, and methods for improving the efficiency of large-area perovskite films for commercial application. The work is structured in three parts. First is a brief overview of large-area PSCs, followed by a discussion of the preparation methods and methods to improve PSC efficiency, quality, and stability. Envisioned future perspectives on the synthesis and commercialization of large-area PSCs are discussed last. Most of the growth in commercial PSC applications is likely to be in building integrated photovoltaics and electric vehicle battery charging solutions. This review concludes that blade coating, slot-die coating, and ink-jet printing carry the highest potential for the scalable manufacture of large-area PSCs with moderate-to-high PCEs. More research and development are key to improving PSC stability and, in the long-term, closing the chasm in lifespan between PSCs and conventional photovoltaic cells.
Surface and Tribological Properties of Oxide Films on Aluminium Alloy through Fly-Ash Reinforcement
Authors: Mat Tahir Noor Ayuma , Liza Shahira , Fukuda Kanao , Mohamad Syazwani , Hashimi Mohd Zakir Fathi , Yunus Mohd Saifulnizam Mohd , Yaakob Yazid , Othman Intan Sharhida
Published: February 2022

Hard anodizing has proven to be a helpful surface treatment for aluminium alloy and typically accompanied by the growth of a porous and highly flawed oxide layer. The presence of pores on the oxide surface can be taken as an advantage in improving the surface properties. Fly-ash particles are high in SiO2 and Al2O3 content and can be utilized as inexpensive strengthening particles, which can increase the wear resistance and microhardness of composite material. It was noticed that limited research had been carried out in utilizing fly-ash as reinforcement on composite oxide coating as a wear resistance candidate. Thus, this study focused on reinforcing fly-ash on oxide coating and investigating its tribological performance. The composite oxide coating was grown on AA2017 aluminium alloy through anodizing process. To understand the effect of anodizing time and fly-ash content, the parameters were varied from 5–60 min and 0–50 g/L, respectively. The findings suggested that 60 min of anodizing time provides the highest thickness and surface roughness at 35 µm and 6.5 µm, respectively. Interestingly, composite oxide coating with 50 g/L fly-ash provides the highest coating thickness but has the lowest roughness at 52 μm and 8.2 μm, respectively. The composite oxide coatings are observed to reduce friction only for a limited time, despite their potential in significantly reducing the wear rate. The wear mechanism observed was adhesion, micro-crack, and delamination. The findings of this study are believed to provide insight on the potential of fly-ash to be a reinforcement for wear-reduction materials.
A LiNbO3 Integrated Optics Sensor for Measurement of Pulsed Current
Authors: Zhang Jiahong , Li Qinkai , Li Yingna , Zhang Jing
Published: February 2022

A LiNbO3 integrated optics sensor fusing with an optical waveguide Mache–Zehnder interferometer (MZI), a loop-antenna, and a segmented electrode has been proposed, designed, and fabricated for the measurement of pulsed current. The experiment results have demonstrated that, for measurement of the standard 8/20 μs lightning pulsed current, the average of the front time and duration time of the sensor detected pulsed current waveform are 7.00 μs and 19.78 μs, respectively, while those detected by the Person CT are 7.30 μs and 20.35 μs, respectively. From 100 A to 3300 A, the sensor shows good linear characteristics, and the correlation coefficient is 0.9989. Moreover, the minimum detectable pulsed current is about 66 A in the time domain. All these results reveal the sensor can provide a new and potential technology for the measurement of pulsed current in the time domain.
Cd4InO(BO3)3: A New Nonlinear Optical Crystal Exhibiting Strong Second Harmonic Generation Effect and Moderate Birefringence
Authors: Liu Runqing , Wu Hongping , Yu Hongwei , Hu Zhanggui , Wang Jiyang , Wu Yicheng
Published: February 2022

A new noncentrosymmetric cadmium indium borate, Cd4InO(BO3)3 (CIBO) has been successfully developed via a standard solid-state reaction. Its crystal structure was confirmed by the single crystal X-ray diffraction, which shows that CIBO belongs to the non-centrosymmetric and polar space group Cm. Its structure contains the distorted InO6 and CdOn (n = 6, 8) polyhedra, which link together by sharing an edge or corner to build a three dimensions framework with BO3 triangles accommodated in tunnels. Benefiting from the approximately parallel configuration of BO3 triangles, CIBO exhibited a strong second harmonic generation (SHG) effect (3 × KDP), and moderate birefringence of 0.077@1064 nm. Further optical and thermal characterizations suggest that CIBO possesses a wide transparent window and good thermal stability. Theoretical calculation reveals that the macroscopic SHG coefficients of CIBO results from the synergistic effect of the parallel arrangement of BO3 groups and d10 Cd2+ cation.
Analysis of Prognostic Risk Factors and Establishment of Prognostic Scoring System for Secondary Adult Hemophagocytic Syndrome
Authors: Zhang Qiaolei , Lin Youyan , Bao Yejiang , Jin Yuan , Ye Xiujin , Tan Yamin
Published: February 2022

Introduction: The objective of this paper is to identify the prognostic risk factors of secondary adult hemophagocytic syndrome (HLH) in hospitalized patients and establish a simple and convenient prognostic scoring system. Method:We reviewed 162 adult patients secondary with HLH treated in Zhejiang Cancer Hospital and the First Affiliated Hospital of Medical College of Zhejiang University from January 2014 to December 2018 were enrolled to form the test group; from January 2019 to February 2021, 162 adult patients in the hospitals constituted the validation group. The HLH prognosis scoring system was constructed according to the risk factors, and the patients were divided into three risk groups: low risk, medium risk, and high risk. The scoring system was verified by Kaplan–Meier method and log rank test survival analysis. The discrimination ability was evaluated according to the receiver operating characteristic (ROC) curve. Results: Univariate and multivariate analysis showed that the independent risk factors for the prognosis of HLH were male sex, activated partial prothrombin time (APTT) greater than 36 s, lactate dehydrogenase (LDH) greater than 1000 U/L, and C-reactive protein (CRP) greater than 100 mg/L. The area under the ROC curve was 0.754 (95% Cl: 0.678–0.829). The patients were divided into a low-risk group (0–1), a medium-risk group (2–4), and a high-risk group (5–6). The 5-year overall survival (OS) rate were 87.5%, 41.8% and 12.8%, respectively (p < 0.001). The area under ROC curve was 0.736 (95% Cl: 0.660–0.813) in the validation group, and the 2-year OS of patients in low-risk, medium-risk and high-risk groups were 88.0%, 45.1% and 16.7%, respectively (p < 0.001). Conclusion:The new prognostic scoring system can accurately predict the prognosis of secondary adult HLH and can further provide basis for the accurate treatment of secondary adult HLH.
Application of a Genetic Algorithm for Proactive Resilient Scheduling in Construction Projects
Authors: Milat Martina , Knezić Snježana , Sedlar Jelena
Published: February 2022

During the execution of construction projects, uncertain events, such as delays, prolongations and disruptions of project activities, have the potential to cause a significant deviation between the planned and realized state of a project. As a result, progress on important project objectives can decrease and this leads to critical delays as well as heavy profit loss. For this reason, we propose the implementation of the customized evolutionary algorithm to generate resilient baseline schedules which include a sufficient number of time floats to absorb the negative impact of uncertainty. This way, the baseline solution is searched as a trade-off between project duration, its final profit and the overall baseline stability. The proposed algorithm is applied to real construction project data and the results of the analysis suggest improved stability for resilient baseline schedules. Application of the genetic algorithm to solve the existing multi-objective problem enables practical implementation of new technologies and methods in construction management. Resilient baseline schedules can be used in an uncertain environment to achieve more accurate predictions and support decision making in the areas of construction scheduling and costing.