bluuebunny/crossref_metadata_2025 huggingface dataset:
Knowledge and Practices on Antibiotic Use and Antibiotic Resistance Among Smallholder Pig Farmers in Timor-Leste
Authors: Ting Shawn , Pereira Abrao , Davis Steven , Vong da Silva Paulo Gabriel , Alves Amalia , Dos Santos Cristibela , Toribio Jenny-Ann L. M. L. , Morais Olavio , da Costa Jong Joanita Bendita , Barnes Tamsin S.
Published: January 2022

Antibiotic resistance is an emerging global health threat which is linked to the overuse and misuse of antibiotics. This study was conducted to understand the knowledge and practices of smallholder pig farmers on antibiotic use and resistance in Timor-Leste. A cross-sectional study using a structured face-to-face interview was conducted in three municipalities. The interview was piloted and implemented in the local Tetun language. This study found that knowledge of antibiotics was very poor as only 12.7% (95% CI: 6.3–23.9) of farmers reported knowing what antibiotics were, and of these only one was able to correctly explain how an antibiotic worked. None of the farmers knew about antibiotic resistance and were able to explain the concept correctly. After the definition of antibiotic was explained to the farmer, only 3.6% (95% CI: 0.8–14.9) reported that their pigs had ever received antibiotics, and the majority of farmers whose pigs had not received antibiotics reported the lack of access to veterinary services. When used, antibiotics were only used for treatment with no reported use for disease prevention or growth promotion. None of the commonly used antibiotics were critically important antimicrobials. Compliance with withdrawal periods was not routinely followed. There is a need to improve access to government veterinary services for farmers in Timor-Leste, while addressing identified knowledge gaps on antibiotics and promoting prudent use practices. The findings from this study serve as baseline information to inform future interventions.
Quaternion versus Rotation Matrix Feedback for Spacecraft Attitude Stabilization Using Magnetorquers
Authors: Celani Fabio
Published: January 2022

The purpose of this paper is to compare performances between stabilization algorithms of quaternion plus attitude rate feedback and rotation matrix plus attitude rate feedback for an Earth-pointing spacecraft with magnetorquers as the only torque actuators. From a mathematical point of view, an important difference between the two stabilizing laws is that only quaternion feedback can exhibit an undesired behavior known as the unwinding phenomenon. A numerical case study is considered, and two Monte Carlo campaigns are carried out: one in nominal conditions and one in perturbed conditions. It turns out that quaternion feedback compares more favorably in terms of the speed of convergence in both campaigns, and it requires less energy in perturbed conditions.
Guidelines for the LTO Noise Assessment of Future Civil Supersonic Aircraft in Conceptual Design
Authors: Piccirillo Grazia , Viola Nicole , Fusaro Roberta , Federico Luigi
Published: January 2022

One of the most critical regulatory issues related to supersonic flight arises from limitations imposed by community noise acceptability. The most efficient way to ensure that future supersonic aircraft will meet low-noise requirements is the verification of noise emissions from the early stages of the design process. Therefore, this paper suggests guidelines for the Landing and Take-Off (LTO) noise assessment of future civil supersonic aircraft in conceptual design. The supersonic aircraft noise model is based on the semi-empirical equations employed in the early versions of the Aircraft NOise Prediction Program (ANOPP) developed by NASA, whereas sound attenuation due to atmospheric absorption has been considered in accordance with SAE ARP 866 B. The simulation of the trajectory leads to the prediction of the aircraft noise level on ground in terms of several acoustic metrics (LAmax, SEL, PNLTM and EPNL). Therefore, a dedicated validation has been performed, selecting the only available supersonic aircraft of the Aircraft Noise and Performance database (ANP), that is, the Concorde, through the matching with Noise Power Distance (NPD) curves for LAmax and SEL, obtaining a maximum prediction error of ±2.19%. At least, an application to departure and approach procedures is reported to verify the first noise estimations with current noise requirements defined by ICAO at the three certification measurement points (sideline, flyover, approach) and to draw preliminary considerations for future low-noise supersonic aircraft design.
Effectiveness of Cover Crop Termination Methods on No-Till Cantaloupe
Authors: Kornecki Ted S. , Kichler Corey M.
Published: January 2022

In a no-till system, there are many different methods available for terminating cover crops. Mechanical termination, utilizing rolling and crimping technology, is one method that injures the plant without cutting the stems. Another popular and commercially available method is mowing, but this can cause problems with cover crop re-growth and loose residue interfering with the planter during cash crop planting. A field experiment was conducted over three growing seasons in northern Alabama to determine the effects of different cover crops and termination methods on cantaloupe yield in a no-till system. Crimson clover, cereal rye, and hairy vetch cover crops were terminated using two different roller-crimpers, including a two-stage roller-crimper for four-wheel tractors and a powered roller-crimper for a two-wheel walk-behind tractor. Cover crop termination rates were evaluated one, two, and three weeks after termination. Three weeks after rolling, a higher termination rate was found for flail mowing (92%) compared to lower termination rates for a two-stage roller (86%) and powered roller-crimper (85%), while the control termination rate was only 49%. There were no significant differences in cantaloupe yield among the rolling treatments, which averaged 38,666 kg ha−1. However, yields were higher for cereal rye and hairy vetch cover crops (41,785 kg ha−1 and 42,000 kg ha−1) compared to crimson clover (32,213 kg ha−1).
Using Live and Video Stimuli to Localize Face and Object Processing Regions of the Canine Brain
Authors: Gillette Kirsten D. , Phillips Erin M. , Dilks Daniel D. , Berns Gregory S.
Published: January 2022

Previous research to localize face areas in dogs’ brains has generally relied on static images or videos. However, most dogs do not naturally engage with two-dimensional images, raising the question of whether dogs perceive such images as representations of real faces and objects. To measure the equivalency of live and two-dimensional stimuli in the dog’s brain, during functional magnetic resonance imaging (fMRI) we presented dogs and humans with live-action stimuli (actors and objects) as well as videos of the same actors and objects. The dogs (n = 7) and humans (n = 5) were presented with 20 s blocks of faces and objects in random order. In dogs, we found significant areas of increased activation in the putative dog face area, and in humans, we found significant areas of increased activation in the fusiform face area to both live and video stimuli. In both dogs and humans, we found areas of significant activation in the posterior superior temporal sulcus (ectosylvian fissure in dogs) and the lateral occipital complex (entolateral gyrus in dogs) to both live and video stimuli. Of these regions of interest, only the area along the ectosylvian fissure in dogs showed significantly more activation to live faces than to video faces, whereas, in humans, both the fusiform face area and posterior superior temporal sulcus responded significantly more to live conditions than video conditions. However, using the video conditions alone, we were able to localize all regions of interest in both dogs and humans. Therefore, videos can be used to localize these regions of interest, though live conditions may be more salient.
Impact of Dietary Sodium Butyrate and Salinomycin on Performance and Intestinal Microbiota in a Broiler Gut Leakage Model
Authors: Naghizadeh Mohammad , Klaver Laura , Schönherz Anna A. , Rani Sundas , Dalgaard Tina Sørensen , Engberg Ricarda Margarete
Published: January 2022

Unfavorable alterations of the commensal gut microbiota and dysbacteriosis is a major health problem in the poultry industry. Understanding how dietary intervention alters the microbial ecology of broiler chickens is important for prevention strategies. A trial was conducted with 672 Ross 308 day-old male broilers fed a basic diet (no additives, control) or the basic diet supplemented with 500 mg/kg encapsulated butyrate or 68 mg/kg salinomycin. Enteric challenge was induced by inclusion of 50 g/kg rye in a grower diet and oral gavage of a 10 times overdose of a vaccine against coccidiosis. Compared to control and butyrate-supplemented birds, salinomycin supplementation alleviated growth depression. Compared to butyrate and non-supplemented control, salinomycin increased potentially beneficial Ruminococcaceae and reduced potentially pathogenic Enterobacteriaceae and counts of Lactobacillus salivarius and Clostridium perfringens. Further, salinomycin supplementation was accompanied by a pH decrease and succinic acid increase in ceca, while coated butyrate (0.5 g/kg) showed no or limited effects. Salinomycin alleviated growth depression and maintained intestinal homeostasis in the challenged broilers, while butyrate in the tested concentration showed limited effects. Thus, further investigations are required to identify optimal dietary inclusion rates for butyrate used as alternative to ionophore coccidiostats in broiler production.
ACSL4 Directs Intramuscular Adipogenesis and Fatty Acid Composition in Pigs
Authors: Ren Hongyan , Zhang Haoyuan , Hua Zaidong , Zhu Zhe , Tao Jiashu , Xiao Hongwei , Zhang Liping , Bi Yanzhen , Wang Heng
Published: January 2022

The intramuscular fat is a major quality trait of meat, affecting sensory attributes such as flavor and texture. Several previous GWAS studies identified Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4) gene as the candidate gene to regulate intramuscular fat content in different pig populations, but the underlying molecular function of ACSL4 in adipogenesis within pig skeletal muscle is not fully investigated. In this study, we isolated porcine endogenous intramuscular adipocyte progenitors and performed ACSL4 loss- and gain-of-function experiments during adipogenic differentiation. Our data showed that ACSL4 is a positive regulator of adipogenesis in intramuscular fat cells isolated from pigs. More interestingly, the enhanced expression of ACSL4 in pig intramuscular adipocytes could increase the cellular content of monounsaturated and polyunsaturated fatty acids, such as gamma-L eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA). The above results not only confirmed the function of ACSL4 in pig intramuscular adipogenesis and meat quality attributes, but also provided new clues for the improvement of the nutritional value of pork for human health.
Length at Maturity, Sex Ratio, and Proportions of Maturity of the Giant Electric Ray, Narcine entemedor, in Its Septentrional Distribution
Authors: Pérez-Palafox Xchel Aurora , Morales-Bojórquez Enrique , Aguirre-Villaseñor Hugo , Cruz-Escalona Víctor Hugo
Published: January 2022

The size at which a certain fraction of a fish population reaches sexual maturity is an important parameter of life history. The estimation of this parameter based on logistic or sigmoid models could provide different ogives and values of length at maturity, which must be analyzed and considered as a basic feature of biological reproduction for the species. A total of 305 individuals of Narcine entemedor (N. entemedor) were obtained from artisanal fisheries in the Bahía de La Paz, Mexico. For the organisms sampled, sexes were determined and total length (TL) in cm was measured from October 2013 to December 2015. The results indicated that the females were larger, ranging from 48.5 cm to 84 cm TL, while males varied from 41.5 cm to 58.5 cm TL. The sex ratio was dominated by males ranging from 45–55 cm TL, while females were more abundant from 60 to 85 cm TL. Mature females were present all year long, exhibiting a continuous annual reproductive cycle. The length at maturity data were described by the Gompertz model with value of 55.87 cm TL. The comparison between models, and the model selection between them, showed that the Gompertz model had maximum likelihood and smaller Akaike information criterion, indicating that this model was a better fit to the maturity proportion data of N. entemedor.
Owner-Perception of the Effects of Two Long-Lasting Dog-Appeasing Pheromone Analog Devices on Situational Stress in Dogs
Authors: Nicolas Céline S. , Espuña Gemma , Girardin Aurélie , Fatjó Jaume , Bowen Jonathan , Monginoux Patricia
Published: January 2022

Devices that release a synthetic analog of the canine-appeasing pheromone can help to relax dogs during stressful situations, but they usually last for only one month. Two new devices with this analog were tested by owners of dogs showing signs of stress in a range of everyday situations: Zenidog™ collar, lasting three months, and Zenidog™ diffusing gel, lasting two months (Virbac, Carros, France). They were compared against reference products that last for one month. In the three-month study with collars, one group received Zenidog™ collar, one received the reference collar, and one group of dogs wore an antiparasitic collar alongside a Zenidog™ collar. In the two-month study with diffusers, groups received either the unpowered Zenidog™ gel diffuser or the reference electric diffuser. Owners regularly completed a questionnaire that assessed seventeen general behaviors and sources of fear and eleven specific signs of stress. Global scores for these two main scales were calculated, and the evolution of scores was compared between groups. Non-parametric tests with a Bonferroni correction were used for statistical analysis. An improvement of all global scores was observed in all groups (p < 0.001), including in puppies, and there was no difference between groups. Zenidog™ devices were as effective as the reference devices and lasted longer.
Supplementation with a Highly Concentrated Docosahexaenoic Acid (DHA) in Non-Proliferative Diabetic Retinopathy: A 2-Year Randomized Double-Blind Placebo-Controlled Study
Authors: Piñas García Purificación , Hernández Martínez Francisco Javier , Aznárez López Núria , Castillón Torre Luis , Tena Sempere Mª Eugenia
Published: January 2022

We assessed the effect of a 2-year supplementation with a highly concentrated docosahexaenoic acid (DHA) product with antioxidant activity on non-proliferative diabetic retinopathy (NPDR) in a randomized double-blind placebo-controlled study. A total of 170 patients with diabetes were randomly assigned to the DHA group (n = 83) or the placebo group (n = 87). NPDR was diagnosed using non-contact slit lamp biomicroscopy examination, and classified into mild, moderate, and severe stages. Patients in the DHA group received a high rich DHA triglyceride (1050 mg/day) nutritional supplement, and those in the placebo group received olive oil capsules. The percentages of mild NPDR increased from 61.7% at baseline to 75.7% at the end of the study in the DHA group, and from 61.9% to 73.1% in the placebo group. Moderate NPDR stages decreased from 35.1% at baseline to 18.7% at the end of the study in the DHA group, and from 36.8% to 26.0% in the placebo group. In the DHA group, there were five eyes with severe NPDR at baseline, which increased to one more at the end of the study. In the placebo group, of two eyes with severe NPDR at baseline, one eye remained at the end of the study. Changes in visual acuity were not found. There were improvements in the serum levels of HbA1c in both groups, but significant differences between the DHA and the placebo groups were not found. In this study, the use of a DHA triglyceride nutraceutical supplement for 2 years did not appear to influence the slowing of the progression of NPDR.
The Concentration-Dependent Effects of Essential Oils on the Growth of Fusarium graminearum and Mycotoxins Biosynthesis in Wheat and Maize Grain
Authors: Gwiazdowska Daniela , Marchwińska Katarzyna , Juś Krzysztof , Uwineza Pascaline Aimee , Gwiazdowski Romuald , Waśkiewicz Agnieszka , Kierzek Roman
Published: January 2022

The presence of Fusarium fungi and their toxic metabolites in agricultural crops contributes to significant quantitative and qualitative losses of crops, causing a direct threat to human and animal health and life. Modern strategies for reducing the level of fungi and mycotoxins in the food chain tend to rely on natural methods, including plant substances. Essential oils (EOs), due to their complex chemical composition, show high biological activity, including fungistatic properties, which means that they exhibit high potential as a biological plant protection factor. The aim of this study was to determine the fungistatic activity of three EOs against F. graminearum, and the reduction of mycotoxin biosynthesis in corn and wheat grain. All tested EOs effectively suppressed the growth of F. graminearum in concentrations of 5% and 10%. Cinnamon and verbena EOs also effectively reduced the ergosterol (ERG) content in both grains at the concentration of 1%, while at the 0.1% EO concentration, the reduction in the ERG amount depended on the EO type as well as on the grain. The degree of zearalenone (ZEA) reduction was consistent with the inhibition of ERG biosynthesis, while the reduction in deoxynivalenol (DON) was not consistent with this parameter.
GSV-NET: A Multi-Modal Deep Learning Network for 3D Point Cloud Classification
Authors: Hoang Long , Lee Suk-Hwan , Lee Eung-Joo , Kwon Ki-Ryong
Published: January 2022

Light Detection and Ranging (LiDAR), which applies light in the formation of a pulsed laser to estimate the distance between the LiDAR sensor and objects, is an effective remote sensing technology. Many applications use LiDAR including autonomous vehicles, robotics, and virtual and augmented reality (VR/AR). The 3D point cloud classification is now a hot research topic with the evolution of LiDAR technology. This research aims to provide a high performance and compatible real-world data method for 3D point cloud classification. More specifically, we introduce a novel framework for 3D point cloud classification, namely, GSV-NET, which uses Gaussian Supervector and enhancing region representation. GSV-NET extracts and combines both global and regional features of the 3D point cloud to further enhance the information of the point cloud features for the 3D point cloud classification. Firstly, we input the Gaussian Supervector description into a 3D wide-inception convolution neural network (CNN) structure to define the global feature. Secondly, we convert the regions of the 3D point cloud into color representation and capture region features with a 2D wide-inception network. These extracted features are inputs of a 1D CNN architecture. We evaluate the proposed framework on the point cloud dataset: ModelNet and the LiDAR dataset: Sydney. The ModelNet dataset was developed by Princeton University (New Jersey, United States), while the Sydney dataset was created by the University of Sydney (Sydney, Australia). Based on our numerical results, our framework achieves more accuracy than the state-of-the-art approaches.
Analysis of the Working Performance of a Back-to-Back Geosynthetic-Reinforced Soil Wall
Authors: Yang Guangqing , Zhao Yunfei , Wang He , Wang Zhijie
Published: January 2022

Back-to-back geosynthetic-reinforced soil walls (BBGRSWs) are commonly used in embankments approaching bridges and narrow spaces. However, the available literature and design guidelines for BBGRSWs are limited. The aims of this research were to develop a greater understanding of the working performance of BBGRSWs and to optimize the design method of a BBGRSW to ensure the cost-efficiency as well as the stability of the structure. On the basis of a monitored BBGRSW structure located in China, we established a numerical model. The parameters of the materials used in the actual project were determined through triaxial and tensile tests. The numerical results were compared with the measured results in the field to verify the correctness of the selected parameters. Two parameters were investigated by the FEM method: the reinforcement length and the arrangement. The FEM analysis indicated that post-construction deformations such as displacement and settlement could be reduced by reinforcing the same layer on both sides. Longer reinforcements were needed to achieve the same performance if the reinforcements were cross-arranged. Thus, BBGRSWs can have a superior performance if the reinforcements are connected in the middle from both sides. Even with longer reinforcements, the safety factor of the wall with a cross-arranged reinforcement was smaller than that with same-layered reinforcements.
Securing SCADA Energy Management System under DDos Attacks Using Token Verification Approach
Authors: Yang Yu-Sheng , Lee Shih-Hsiung , Chen Wei-Che , Yang Chu-Sing , Huang Yuen-Min , Hou Ting-Wei
Published: January 2022

The advanced connection requirements of industrial automation and control systems have sparked a new revolution in the Industrial Internet of Things (IIoT), and the Supervisory Control and Data Acquisition (SCADA) network has evolved into an open and highly interconnected network. In addition, the equipment of industrial electronic devices has experienced complete systemic integration by connecting with the SCADA network, and due to the control and monitoring advantages of SCADA, the interconnectivity and working efficiency among systems have been tremendously improved. However, it is inevitable that the SCADA system cannot be separated from the public network, which indicates that there are concerns over cyber-attacks and cyber-threats, as well as information security breaches, in the SCADA network system. According to this context, this paper proposes a module based on the token authentication service to deter attackers from performing distributed denial-of-service (DDoS) attacks. Moreover, a simulated experiment has been conducted in an energy management system in the actual field, and the experimental results have suggested that the security defense architecture proposed by this paper can effectively improve security and is compatible with real field systems.
Model to Program and Blockchain Approaches for Business Processes and Workflows in Finance
Authors: Kherbouche Meriem , Pisoni Galena , Molnár Bálint
Published: January 2022

Business process modeling and verification have become an essential way to control and assure organizational evolution. We overview the opportunities for the application of blockchain in Business Process Management and Modeling in Finance and we focus on in-depth analysis of claim process in insurance as a use case. We investigate the utilization of blockchain technology for model checking of Workflow, Business Processes to ensure consistency, integrity, and security in a dynamically changing business environment. We create a UML profile for the blockchain, then we combine it with a UML activity diagram followed by a verification using Petri nets to guarantee a distributed computing system and scalable with mutable data. Our paper creates a unified picture of the approaches towards business processes modeling used in the financial industry organized around the set of premises intending to develop a future research agenda for blockchain business process modeling, specifically for the financial industry domain.
The Controlling of the Subtropical High Leading Modes on the Spatial Pattern of Tropical Cyclone Genesis in the Western North Pacific and Tracks Landing on the East Coast of China
Authors: Fan Tingting , Yang Yuxing , Xu Shibin
Published: January 2022

As a prime circulation system, the western Pacific subtropical high (WPSH) significantly impacts tropical cyclone (TC) activities over the western North Pacific (WNP), especially TCs landing on the east coast of China; however, the associated mechanism is not firmly established. This study investigates the underlying dynamic impact of the first two empirical orthogonal function (EOF) modes of the WPSH on the interannual variability in the genesis and number of TCs landing over the WNP. The results show that these two dominant modes control the WNP TC activity over different subregions via different environmental factors. The first mode (EOF1) affects the TC genesis number over region I (105°–128° E, 5°–30° N) (r = −0.49) and region II (130°–175° E, 17°–30° N) (r = −0.5) and controls the TCs landing on the east coast of China, while the second mode (EOF2) affects the TC genesis number over region III (128°–175° E, 5°–17° N) (r = −0.69). The EOF1 mode, a southwest-northeast-oriented enhanced pattern, causes the WPSH to expand (retreat) along the southwest-northeast direction, which makes both mid-low-level relative humidity and low-level vorticity unfavorable (favorable) for TC genesis in region I and region II and steers fewer (more) TC tracks to land on the coast of China. The EOF2 mode features a strengthened WPSH over the southeast quarter of the WNP region. The active (inactive) phases of this mode control the low-level vorticity and vertical wind shear in region III, which lead to less (more) TC genesis over this region. The prediction equations combining the two modes of the WPSH for the total number of TCs and TCs that make landfall show high correlation coefficients. Our findings verify the high prediction skill of the WPSH on WNP TC activities, provide a new way to predict TCs that will make landfall on the east coast of China, and help to improve the future projection of WNP TC activity.
A Characterization and Cell Toxicity Assessment of Particulate Pollutants from Road Traffic Sites in Kano State, Nigeria
Authors: Sadiq Aishatu , Khardi Salah , Lazar Adina-Nicoleta , Bello Imam , Salam Samson , Faruk Aisha , Alao Mitchell , Catinon Mickaël , Vincent Michel , Trunfio-Sfarghiu Ana-Maria
Published: January 2022

Emerging African countries are characterized by explosive population growth and urbanization, which threaten environmental sustainability. This study comparatively characterized ambient aerosols and assessed cytotoxicity to facilitate improving health and environmental policy. Twenty-four air samples were collected at high and low-density traffic sites in Kano State using polysulfone and stainless steel filters attached to an automated pump. The physico-chemical properties of particulate matter were determined using scanning electron microscopy and energy dispersive X-ray analysis (SEM-EDX). In vitro, their potential toxicity was assessed using macrophages and cell fixation with staining. Results showed 51.7% of particles as PM2.5, with the highest particle concentration in mixed sites (urban and industrial). Particle classification into four groups by elemental composition and structure showed: sand particles (Si, Al, Fe, Ca, Mg, K, Na, Mo, Sr, Zr) 30–51%; other fibers 0–3%; other particles (Si, Fe, S, Mo, Zn, and other metals) 22–40%; and silicone-based fibres 23–34%. The abundant elements are: Si, Al, Ca, Ce, Ti, Fe, Cl, Pb, and Mn. The lowest viability on cytotoxicity assessment was recorded in mixed site M2. The majority of households were located within 50 m of air sampling sites. Proximity to traffic sites worsens health, as evidenced in cytotoxicity findings. We recommend improved urban planning and intensification of emissions control.
Sensory Profiling and External Preference Mapping of Pre-Thickened Water Products for Dysphagia
Authors: Chambers Edgar , Garcia Jane Mertz , Li Han
Published: January 2022

Dysphagia is a dysfunction of the swallowing system and thickened beverages are widely prescribed as a treatment. The objective of this study was to determine the sensory characteristics of pre-thickened water products and determine sensory drivers of liking for those types of products. Pre-thickened water products (two starch-based and two gum-based) were tested in both nectar and honey thick versions. Based on product availability, one product was neutral water, and the others were water with lemon flavoring added. The sensory characteristics of the products were evaluated with a highly trained descriptive panel, and the viscosity of the products was evaluated with both the Bostwick consistometer and the line spread test. Previously published consumer data was used to determine preference maps with the descriptive data. All lemon-flavored products had similar flavor characteristics, except the Thick & Easy Clear that had a less lemon-related flavor than the others. After excluding the lemon-related flavor characteristics, all pre-thickened products had similar “base flavor” notes, no matter whether the products were starch-based or gum-based. This contrasts with literature, which reports differences in flavor of thickened beverages prepared using thickening agents. The thickness of the products varied between nectar and honey thickness, but also varied among products that were stated to be at the same level of thickness. External preference mapping showed that both lemon-related flavor and a thinner beverage texture are potential drivers of consumers acceptance of the product, which was expected based on other studies. In conclusion, pre-thickened beverages are good alternatives for self-mixed thickening agents but a standard for thickness should be agreed on and used by manufacturers.
Characterization of Musts, Wines, and Sparkling Wines Based on Their Elemental Composition Determined by ICP-OES and ICP-MS
Authors: Granell Biel , Izquierdo-Llopart Anaïs , Sahuquillo Àngels , López-Sánchez José F. , Saurina Javier
Published: January 2022

Samples from the different processing stages in the elaboration of sparkling wine (cava)—including must, base wine, and sparkling wine—of Pinot Noir and Xarel·lo grape varieties from different vineyard qualities (A, B, C, D) have been analyzed by inductively coupled plasma (ICP) techniques to determine their elemental composition. The resulting data has been used to characterize these products according to oenological features and product qualities. For this purpose, box plot diagrams, bar charts, and principal components analysis (PCA) have been used. The study of the behavior of each given species has pointed out the relevance of some elements as markers or descriptors of winemaking processes. Among others, Cu and K are abundant in musts and their concentrations progressively decrease through the cava production process. S levels suddenly increase at the base wine step (and further decay) due to the addition of sulfites as preserving agents. Finally, concentrations of Na, Ca, Fe, and Mg increase from the first fermentation due to the addition of clarifying agents such as bentonite. PCA has been applied to try to extract solid and global conclusions on trends and chemical markers within the groups of samples more easily and efficiently than more conventional approaches.
NOG-Derived Peptides Can Restore Neuritogenesis on a CRASH Syndrome Cell Model
Authors: Gasparotto Matteo , Hernandez Gomez Yuriko Suemi , Peterle Daniele , Grinzato Alessandro , Zen Federica , Pontarollo Giulia , Acquasaliente Laura , Scapin Giorgia , Bergantino Elisabetta , De Filippis Vincenzo , Filippini Francesco
Published: January 2022

Homo- and heterophilic binding mediated by the immunoglobulin (Ig)-like repeats of cell adhesion molecules play a pivotal role in cell-cell and cell-extracellular matrix interactions. L1CAM is crucial to neuronal differentiation, in both mature and developing nervous systems, and several studies suggest that its functional interactions are mainly mediated by Ig2–Ig2 binding. X-linked mutations in the human L1CAM gene are summarized as L1 diseases, including the most diagnosed CRASH neurodevelopmental syndrome. In silico simulations provided a molecular rationale for CRASH phenotypes resulting from mutations I179S and R184Q in the homophilic binding region of Ig2. A synthetic peptide reproducing such region could both mimic the neuritogenic capacity of L1CAM and rescue neuritogenesis in a cellular model of the CRASH syndrome, where the full L1CAM ectodomain proved ineffective. Presented functional evidence opens the route to the use of L1CAM-derived peptides as biotechnological and therapeutic tools.
Clinical Genetics of Inherited Arrhythmogenic Disease in the Pediatric Population
Authors: Martínez-Barrios Estefanía , Cesar Sergi , Cruzalegui José , Hernandez Clara , Arbelo Elena , Fiol Victoria , Brugada Josep , Brugada Ramon , Campuzano Oscar , Sarquella-Brugada Georgia
Published: January 2022

Sudden death is a rare event in the pediatric population but with a social shock due to its presentation as the first symptom in previously healthy children. Comprehensive autopsy in pediatric cases identify an inconclusive cause in 40–50% of cases. In such cases, a diagnosis of sudden arrhythmic death syndrome is suggested as the main potential cause of death. Molecular autopsy identifies nearly 30% of cases under 16 years of age carrying a pathogenic/potentially pathogenic alteration in genes associated with any inherited arrhythmogenic disease. In the last few years, despite the increasing rate of post-mortem genetic diagnosis, many families still remain without a conclusive genetic cause of the unexpected death. Current challenges in genetic diagnosis are the establishment of a correct genotype–phenotype association between genes and inherited arrhythmogenic disease, as well as the classification of variants of uncertain significance. In this review, we provide an update on the state of the art in the genetic diagnosis of inherited arrhythmogenic disease in the pediatric population. We focus on emerging publications on gene curation for genotype–phenotype associations, cases of genetic overlap and advances in the classification of variants of uncertain significance. Our goal is to facilitate the translation of genetic diagnosis to the clinical area, helping risk stratification, treatment and the genetic counselling of families.
Experimental Volumetric Hydrogen Uptake Determination at 77 K of Commercially Available Metal-Organic Framework Materials
Authors: Villajos Jose A.
Published: January 2022

Storage is still limiting the implementation of hydrogen as an energy carrier to integrate the intermittent operation of renewable energy sources. Among different solutions to the currently used compressed or liquified hydrogen systems, physical adsorption at cryogenic temperature in porous materials is an attractive alternative due to its fast and reversible operation and the resulting reduction in storage pressure. The feasibility of cryoadsorption for hydrogen storage depends mainly on the performance of the used materials for the specific application, where metal-organic frameworks or MOFs are remarkable candidates. In this work, gravimetric and volumetric hydrogen uptakes at 77 K and up to 100 bar of commercially available MOFs were measured since these materials are made from relatively cheap and accessible building blocks. These materials also show relatively high porous properties and are currently near to large-scale production. The measuring device was calibrated at different room temperatures to calculate an average correction factor and standard deviation so that the correction deviation is included in the measurement error for better comparability with different measurements. The influence of measurement conditions was also studied, concluding that the available adsorbing area of material and the occupied volume of the sample are the most critical factors for a reproducible measurement, apart from the samples’ preparation before measurement. Finally, the actual volumetric storage density of the used powders was calculated by directly measuring their volume in the analysis cell, comparing that value with the maximum volumetric uptake considering the measured density of crystals. From this selection of commercial MOFs, the materials HKUST-1, PCN-250(Fe), MOF-177, and MOF-5 show true potential to fulfill a volumetric requirement of 40 g·L−1 on a material basis for hydrogen storage systems without further packing of the powders.
Contribution of LAT1-4F2hc in Urological Cancers via Toll-like Receptor and Other Vital Pathways
Authors: Zhao Xue , Sakamoto Shinichi , Maimaiti Maihulan , Anzai Naohiko , Ichikawa Tomohiko
Published: January 2022

Tumor cells are known for their ability to proliferate. Nutrients are essential for rapidly growing tumor cells. In particular, essential amino acids are essential for tumor cell growth. Tumor cell growth nutrition requires the regulation of membrane transport proteins. Nutritional processes require amino acid uptake across the cell membrane. Leucine, one of the essential amino acids, has recently been found to be closely associated with cancer, which activate mTOR signaling pathway. The transport of leucine into cells requires an L-type amino acid transporter protein 1, LAT1 (SLC7A5), which requires the 4F2 cell surface antigen heavy chain (4F2hc, SLC3A2) to form a heterodimeric amino acid transporter protein complex. Recent evidence identified 4F2hc as a specific downstream target of the androgen receptor splice variant 7 (AR-V7). We stressed the importance of the LAT1-4F2hc complex as a diagnostic and therapeutic target in urological cancers in this review, which covered the recent achievements in research on the involvement of the LAT1-4F2hc complex in urinary system tumors. In addition, JPH203, which is a selective LAT1 inhibitor, has shown excellent inhibitory effects on the proliferation in a variety of tumor cells. The current phase I clinical trials of JPH203 in patients with biliary tract cancer have also achieved good results, which is the future research direction for LAT1 targeted therapy drugs.
Metabolic Reprogramming and Risk Stratification of Hepatocellular Carcinoma Studied by Using Gas Chromatography–Mass Spectrometry-Based Metabolomics
Authors: Fang Chengnan , Wang Hui , Lin Zhikun , Liu Xinyu , Dong Liwei , Jiang Tianyi , Tan Yexiong , Ning Zhen , Ye Yaorui , Tan Guang , Xu Guowang
Published: January 2022

Hepatocellular carcinoma (HCC) displays a high degree of metabolic and phenotypic heterogeneity and has dismal prognosis in most patients. Here, a gas chromatography–mass spectrometry (GC-MS)-based nontargeted metabolomics method was applied to analyze the metabolic profiling of 130 pairs of hepatocellular tumor tissues and matched adjacent noncancerous tissues from HCC patients. A total of 81 differential metabolites were identified by paired nonparametric test with false discovery rate correction to compare tumor tissues with adjacent noncancerous tissues. Results demonstrated that the metabolic reprogramming of HCC was mainly characterized by highly active glycolysis, enhanced fatty acid metabolism and inhibited tricarboxylic acid cycle, which satisfied the energy and biomass demands for tumor initiation and progression, meanwhile reducing apoptosis by counteracting oxidative stress. Risk stratification was performed based on the differential metabolites between tumor and adjacent noncancerous tissues by using nonnegative matrix factorization clustering. Three metabolic clusters displaying different characteristics were identified, and the cluster with higher levels of free fatty acids (FFAs) in tumors showed a worse prognosis. Finally, a metabolite classifier composed of six FFAs was further verified in a dependent sample set to have potential to define the patients with poor prognosis. Together, our results offered insights into the molecular pathological characteristics of HCC.
Secretory Autophagy Forges a Therapy Resistant Microenvironment in Melanoma
Authors: Bustos Silvina Odete , Leal Santos Nathalia , Chammas Roger , Andrade Luciana Nogueira de Sousa
Published: January 2022

Melanoma is the most aggressive skin cancer characterized by high mutational burden and large heterogeneity. Cancer cells are surrounded by a complex environment, critical to tumor establishment and progression. Thus, tumor-associated stromal components can sustain tumor demands or impair cancer cell progression. One way to manage such processes is through the regulation of autophagy, both in stromal and tumor cells. Autophagy is a catabolic mechanism that provides nutrients and energy, and it eliminates damaged organelles by degradation and recycling of cellular elements. Besides this primary function, autophagy plays multiple roles in the tumor microenvironment capable of affecting cell fate. Evidence demonstrates the existence of novel branches in the autophagy system related to cytoplasmic constituent’s secretion. Hence, autophagy-dependent secretion assembles a tangled network of signaling that potentially contributes to metabolism reprogramming, immune regulation, and tumor progression. Here, we summarize the current awareness regarding secretory autophagy and the intersection with exosome biogenesis and release in melanoma and their role in tumor resistance. In addition, we present and discuss data from public databases concerning autophagy and exosome-related genes as important mediators of melanoma behavior. Finally, we will present the main challenges in the field and strategies to translate most of the pre-clinical findings to clinical practice.
Recent Advances in the Diagnosis and Management of Multiple Primary Lung Cancer
Authors: Chiang Chi-Lu , Tsai Ping-Chung , Yeh Yi-Chen , Wu Yuan-Hung , Hsu Han-Shui , Chen Yuh-Min
Published: January 2022

With the wide application of computed tomography in lung cancer screening, the incidence of multiple primary lung cancer (MPLC) has been increasingly reported. Despite the established criteria, the differentiation between MPLC and intrapulmonary metastasis remains challenging. Although histologic features are helpful in some circumstances, a molecular analysis is often needed. The application of next-generation sequencing could aid in distinguishing MPLCs from intrapulmonary metastasis, decreasing ambiguity. For MPLC management, surgery with lobectomy is the main operation method. Limited resection does not appear to negatively affect survival, and it is a reasonable alternative. Stereotactic ablative radiotherapy (SABR) has become a standard of care for patients refusing surgery or for those with medically inoperable early-stage lung cancer. However, the efficacy of SABR in MPLC management could only be found in retrospective series. Other local ablation techniques are an emerging alternative for the control of residual lesions. Furthermore, systemic therapies, such as targeted therapy for oncogene-addicted patients, and immunotherapy have shown promising results in MPLC management after resection. In this paper, the recent advances in the diagnosis and management of MPLC are reviewed.
Comparative O-GlcNAc Proteomic Analysis Reveals a Role of O-GlcNAcylated SAM68 in Lung Cancer Aggressiveness
Authors: Lin Chia-Hung , Liao Chen-Chung , Wang Shu-Ying , Peng Chia-Yi , Yeh Yi-Chen , Chen Mei-Yu , Chou Teh-Ying
Published: January 2022

O-GlcNAcylation is a reversible and dynamic post-translational protein modification catalyzed by O-GlcNAc transferase (OGT). Despite the reported association of O-GlcNAcylation with cancer metastasis, the O-GlcNAc proteome profile for cancer aggressiveness remains largely uncharacterized. Here, we report our comparative O-GlcNAc proteome profiling of two differentially invasive lung adenocarcinoma cell lines, which identified 158 down-regulated and 106 up-regulated candidates in highly invasive cells. Among these differential proteins, a nuclear RNA-binding protein, SAM68 (SRC associated in mitosis of 68 kDa), was further investigated. Results showed that SAM68 is O-GlcNAcylated and may interact with OGT in the nucleus. Eleven O-GlcNAcylation sites were identified, and data from mutant analysis suggested that multiple serine residues in the N-terminal region are important for O-GlcNAcylation and the function of SAM68 in modulating cancer cell migration and invasion. Analysis of clinical specimens found that high SAM68 expression was associated with late cancer stages, and patients with high-OGT/high-SAM68 expression in their tumors had poorer overall survival compared to those with low-OGT/low-SAM68 expression. Our study revealed an invasiveness-associated O-GlcNAc proteome profile and connected O-GlcNAcylated SAM68 to lung cancer aggressiveness.
Glutamine-Derived Aspartate Biosynthesis in Cancer Cells: Role of Mitochondrial Transporters and New Therapeutic Perspectives
Authors: Gorgoglione Ruggiero , Impedovo Valeria , Riley Christopher L. , Fratantonio Deborah , Tiziani Stefano , Palmieri Luigi , Dolce Vincenza , Fiermonte Giuseppe
Published: January 2022

Aspartate has a central role in cancer cell metabolism. Aspartate cytosolic availability is crucial for protein and nucleotide biosynthesis as well as for redox homeostasis. Since tumor cells display poor aspartate uptake from the external environment, most of the cellular pool of aspartate derives from mitochondrial catabolism of glutamine. At least four transporters are involved in this metabolic pathway: the glutamine (SLC1A5_var), the aspartate/glutamate (AGC), the aspartate/phosphate (uncoupling protein 2, UCP2), and the glutamate (GC) carriers, the last three belonging to the mitochondrial carrier family (MCF). The loss of one of these transporters causes a paucity of cytosolic aspartate and an arrest of cell proliferation in many different cancer types. The aim of this review is to clarify why different cancers have varying dependencies on metabolite transporters to support cytosolic glutamine-derived aspartate availability. Dissecting the precise metabolic routes that glutamine undergoes in specific tumor types is of upmost importance as it promises to unveil the best metabolic target for therapeutic intervention.
Cause of Death, Mortality and Occult Blood in Colorectal Cancer Screening
Authors: Kaalby Lasse , Al-Najami Issam , Deding Ulrik , Berg-Beckhoff Gabriele , Steele Robert J. C. , Kobaek-Larsen Morten , Shaukat Aasma , Rasmussen Morten , Baatrup Gunnar
Published: January 2022

Fecal hemoglobin (f-Hb) detected by the guaiac fecal occult blood test (gFOBT) may be associated with mortality and cause of death in colorectal cancer (CRC) screening participants. We investigated this association in a randomly selected population of 20,694 participants followed for 33 years. We followed participants from the start of the Hemoccult-II CRC trial in 1985–1986 until December 2018. Data on mortality, cause of death and covariates were retrieved using Danish national registers. We conducted multivariable Cox regressions with time-varying exposure, reporting results as crude and adjusted hazard ratios (aHRs). We identified 1766 patients with at least one positive gFOBT, 946 of whom died in the study period. Most gFOBT-positive participants (93.23%) died of diseases unrelated to CRC and showed higher non-CRC mortality than gFOBT-negative participants (aHR: 1.20, 95% CI 1.10–1.30). Positive gFOBT participants displayed a modest increase in all-cause (aHR: 1.28, 95% CI: 1.18–1.38), CRC (aHR: 4.07, 95% CI: 3.00–5.56), cardiovascular (aHR: 1.22, 95% CI: 1.07–1.39) and endocrine and hematological mortality (aHR: 1.58, 95% CI: 1.19–2.10). In conclusion, we observed an association between positive gFOBT, cause of death and mortality. The presence of f-Hb in the gFOBT might indicate the presence of systemic diseases.