bluuebunny/crossref_metadata_2025 huggingface dataset:
Exchange Bias Effect in LaFeO3: La0.7Ca0.3MnO3 Composite Thin Films
Authors: Wang Feng , Fu Wei , Jiang Chengming , Li Junxiao , Huang Jijie
Published: September 2021

Composite thin films arouse great interests owing to the multifunctionalities and heterointerface induced physical property tailoring. The exchange bias effect aroused from the ferromagnetic (FM)–antiferromagnetic (AFM) heterointerface is applicable in various applications such as magnetic storage. In this work, (LaFeO3)x:(La0.7Ca0.3MnO3)1−x composite thin films have been deposited via pulsed laser deposition (PLD) and the exchange bias effect was investigated. In such system, LaFeO3 (LFO) is an antiferromagnet while La0.7Ca0.3MnO3 (LCMO) is a ferromagnet, which results in the exchange bias interfacial coupling at the FM/AFM interface. The composition variation of the two phases could lead to the exchange bias field (HEB) tuning in the composite system. This work demonstrates a new composite thin film system with FM-AFM interfacial exchange coupling, which could be applied in various spintronic applications.
Ecofriendly Ultrasonic Rust Removal: An Empirical Optimization Based on Response Surface Methodology
Authors: Zhang Lijie , He Bing , Wang Shengnan , Wang Guangcun , Yuan Xiaoming
Published: September 2021

This study shows that the hard-to-remove rust layer on the guide sleeve surface of a used cylinder can be removed using a specially developed, environmentally friendly formula for cleaning rust. Furthermore, we studied the rust removal technology that is based on ultrasonic cavitation and chemical etching. The surface morphology and structural components of the rust layer were observed using an electron microscope and an X-ray powder diffractometer. These tools were used to explore the mechanism of combined rust removal. Using response surface methodology (RSM) and central composite design (CCD), with the rust removal rate as our index of evaluation, data were analyzed to establish a response surface model that can determine the effect of cleaning temperature and ultrasonic power interaction on the rate of rust removal. Results showed that the main components of the rust layer on a 45 steel guide sleeve were α-FeOOH, γ-FeOOH, and Fe3O4. The rust was unevenly distributed with a loose structure, which was easily corroded by chemical reagents and peeled off under ultrasonic cavitation. With the increase in the cleaning temperature, the chemical reaction effect was intensified, and the cleaning ability was enhanced. With the increase in ultrasonic power, the cavitation effect was aggravated, the ultrasonic agitation was enhanced, and the rust removal rate was improved. According to response surface analysis and the application scope of the rust remover, we determined that the optimal cleaning temperature is 55 °C, and that the optimal ultrasonic power is 2880 W. The descaling rate under these parameters is 0.15 g·min−1·m−2.
Nanomechanical Concepts in Magnetically Guided Systems to Investigate the Magnetic Dipole Effect on Ferromagnetic Flow Past a Vertical Cone Surface
Authors: Usman Auwalu Hamisu , Shah Zahir , Kumam Poom , Khan Waris , Humphries Usa Wannasingha
Published: September 2021

Because of the floating magnetic nanomaterial, ferrofluids have magneto-viscous properties, enabling controllable temperature changes as well as nano-structured fluid characteristics. The study’s purpose is to evolve and solve a theoretical model of bioconvection nanofluid flow with a magnetic dipole effect in the presence of Curie temperature and using the Forchheimer-extended Darcy law subjected to a vertical cone surface. The model also includes the nonlinear thermal radiation, heat suction/injection, viscous dissipation, and chemical reaction effects. The developed model problem is transformed into nonlinear ordinary differentials, which have been solved using the homotopy analysis technique. In this problem, the behavior of function profiles are graphically depicted and explained for a variety of key parameters. For a given set of parameters, tables representthe expected numerical values and behaviors of physical quantities. The nanofluid velocity decreases as the ferrohydrodynamic, local inertia, and porosity parameters increase and decrease when the bioconvection Rayleigh number increases. Many key parameters improved the thermal boundary layer and temperature. The concentration is low when the chemical reaction parameter and Schmidt number rises. Furthermore, as the bioconvection constant, Peclet and Lewis numbers rise, so does the density of motile microorganisms.
Device for Suppression of Aerosol Transfer in Close Proximity Settings
Authors: Bao Yicheng , Anderegg Loïc , Burchesky Sean , Doyle John M.
Published: September 2021

Here we present a device that suppresses transfer of aerosol between nearby seating areas through the use of optically transparent, sound transmitting barriers and HEPA fan filter unit (FFU). A potential application of this device is to lower the risk of respiratory disease transmission in face-to-face, maskless meetings between individuals in a university setting. We evaluate overall aerosol transmission between users of the device. This is done for two different physical settings: a large space, such as a library, and a small space, such as an enclosed study room. We find that the device can provide lower aerosol transmission compared to the typical transmission between two individuals wearing surgical face masks separated by six feet.
Detection of Cervical Cancer Cells in Whole Slide Images Using Deformable and Global Context Aware Faster RCNN-FPN
Authors: Li Xia , Xu Zhenhao , Shen Xi , Zhou Yongxia , Xiao Binggang , Li Tie-Qiang
Published: September 2021

Cervical cancer is a worldwide public health problem with a high rate of illness and mortality among women. In this study, we proposed a novel framework based on Faster RCNN-FPN architecture for the detection of abnormal cervical cells in cytology images from a cancer screening test. We extended the Faster RCNN-FPN model by infusing deformable convolution layers into the feature pyramid network (FPN) to improve scalability. Furthermore, we introduced a global contextual aware module alongside the Region Proposal Network (RPN) to enhance the spatial correlation between the background and the foreground. Extensive experimentations with the proposed deformable and global context aware (DGCA) RCNN were carried out using the cervical image dataset of “Digital Human Body” Vision Challenge from the Alibaba Cloud TianChi Company. Performance evaluation based on the mean average precision (mAP) and receiver operating characteristic (ROC) curve has demonstrated considerable advantages of the proposed framework. Particularly, when combined with tagging of the negative image samples using traditional computer-vision techniques, 6–9% increase in mAP has been achieved. The proposed DGCA-RCNN model has potential to become a clinically useful AI tool for automated detection of cervical cancer cells in whole slide images of Pap smear.
Environmental Pressures on Top-Down and Bottom-Up Forces in Coastal Ecosystems
Authors: Blum Michael J.
Published: September 2021

Global change is manifesting new and potent pressures that may determine the relative influence of top-down and bottom-up forces on the productivity of plants that undergird coastal ecosystems. Here, I present a meta-analysis conducted to assess how herbivory, nitrogen enrichment, and elevated salinity influence plant productivity according to the salinity regimes of coastal ecosystems. An examination of 99 studies representing 288 effect sizes across 76 different plant species revealed that elevated salinity negatively affected productivity across all environments, but particularly in freshwater ecosystems. Nitrogen enrichment, on the other hand, positively affected productivity. In agreement with the plant stress hypothesis, herbivory had the greatest negative impact in saline habitats. This trend, however, appears to reverse with nitrogen enrichment, with maximum losses to herbivory occurring in brackish habitats. These findings demonstrate that multiple stressors can yield complex, and sometimes opposite outcomes to those arising from individual stressors. This study also suggests that trophic interactions will likely shift as coastal ecosystems continue to experience nutrient enrichment and sea level rise.
Development of Two-Tube Loop-Mediated Isothermal Amplification Assay for Differential Diagnosis of Plasmodium falciparum and Plasmodium vivax and Its Comparison with Loopamp™ Malaria
Authors: Azam Mudsser , Upmanyu Kirti , Gupta Ratan , Sruthy Karugatharayil Sasi , Matlani Monika , Savargaonkar Deepali , Singh Ruchi
Published: September 2021

To strengthen malaria surveillance, field-appropriate diagnostics requiring limited technical resources are of critical significance. Loop-mediated isothermal amplification (LAMP) based malaria diagnostic assays are potential point-of-care tests with high sensitivity and specificity and have been used in low-resource settings. Plasmodium vivax–specific consensus repeat sequence (CRS)-based and Plasmodium falciparum–specific 18S rRNA primers were designed, and a two-tube LAMP assay was developed. The diagnostic performance of a closed-tube LAMP assay and Loopamp™ Malaria Detection (Pan/Pf, Pv) kit was investigated using nested PCR confirmed mono- and co-infections of P. vivax and P. falciparum positive (n = 149) and negative (n = 67) samples. The closed-tube Pv LAMP assay showed positive amplification in 40 min (limit of detection, LOD 0.7 parasites/µL) and Pf LAMP assay in 30 min (LOD 2 parasites/µL). Pv LAMP and Pf LAMP demonstrated a sensitivity and specificity of 100% (95% CI, 95.96–100% and 89.85–100%, respectively). The LoopampTM Pan/Pf Malaria Detection kit demonstrated a sensitivity and specificity of 100%, whereas LoopampTM Pv showed a sensitivity of 98.36% (95% CI, 91.28–99.71%) and specificity of 100% (95% CI, 87.54–100%). The developed two-tube LAMP assay is highly sensitive (LOD ≤ 2 parasite/µL), demonstrating comparable results with the commercial Loopamp™ Malaria Detection (Pf/pan) kit, and was superior in detecting the P. vivax co-infection that remained undetected by the Loopamp™ Pv kit. The developed indigenous two-tube Pf/Pv malaria detection can reliably be used for mass screening in resource-limited areas endemic for both P. falciparum and P. vivax malaria.
Development of Online Virtual Laboratory Platform for Supporting Real Laboratory Experiments in Multi Domains
Authors: Ahmed Mohamed Elsayed , Hasegawa Shinobu
Published: September 2021

The increasing use of online virtual laboratories (OVLs) in educational institutions as a recent educational technology application necessitates developing a new educational platform for assisting instructors in using such technology in the teaching process without web programming obstacles. The OVLs are online environments that provide students with several types of content such as simulations, videos, scientific images, and infographics related to real laboratory experiments. This article proposes a unified online virtual laboratory platform (OVLP) to support instructors who teach real laboratory experiments in multi-domains. To evaluate the proposed platform, five university instructors and five experts of ICT in education have participated in this study. The data were collected using online questionnaires for both specialists, respectively. Regarding the results, they confirm that the proposed platform is acceptable for teaching real laboratory experiments, especially in the tested domains.
A Systematic Literature Review of Student’ Performance Prediction Using Machine Learning Techniques
Authors: Albreiki Balqis , Zaki Nazar , Alashwal Hany
Published: September 2021

Educational Data Mining plays a critical role in advancing the learning environment by contributing state-of-the-art methods, techniques, and applications. The recent development provides valuable tools for understanding the student learning environment by exploring and utilizing educational data using machine learning and data mining techniques. Modern academic institutions operate in a highly competitive and complex environment. Analyzing performance, providing high-quality education, strategies for evaluating the students’ performance, and future actions are among the prevailing challenges universities face. Student intervention plans must be implemented in these universities to overcome problems experienced by the students during their studies. In this systematic review, the relevant EDM literature related to identifying student dropouts and students at risk from 2009 to 2021 is reviewed. The review results indicated that various Machine Learning (ML) techniques are used to understand and overcome the underlying challenges; predicting students at risk and students drop out prediction. Moreover, most studies use two types of datasets: data from student colleges/university databases and online learning platforms. ML methods were confirmed to play essential roles in predicting students at risk and dropout rates, thus improving the students’ performance.
Observer Design for a Variable Moment of Inertia System
Authors: Baranowski Jerzy
Published: September 2021

Variable moment of inertia systems are common, and a popular laboratory system of this type is the “ball-and-beam”. Such systems are, however, nonlinear and often unstable. Efficient control requires full state information (or at least partial velocities), which are generally difficult to measure. That is why the design of state observers is a relevant problem. In this paper, a new design of an observer is proposed. This new nonlinear observer uses partial output injection and the circle criterion to ensure semiglobal stability. Moreover, we present a complete modeling of the system and systematic testing of the observer in comparison to a baseline in the form of a linear observer. The results show that the designed observer outperforms its linear counterpart and does not impede control.
Home Energy Management Systems with Branch-and-Bound Model-Based Predictive Control Techniques
Authors: Bot Karol , Laouali Inoussa , Ruano António , Ruano Maria da Graça
Published: September 2021

At a global level, buildings constitute one of the most significant energy-consuming sectors. Current energy policies in the EU and the U.S. emphasize that buildings, particularly those in the residential sector, should employ renewable energy and storage and efficiently control the total energy system. In this work, we propose a Home Energy Management System (HEMS) by employing a Model-Based Predictive Control (MBPC) framework, implemented using a Branch-and-Bound (BAB) algorithm. We discuss the selection of different parameters, such as time-step, to employ prediction and control horizons and the effect of the weather in the system performance. We compare the economic performance of the proposed approach against a real PV-battery system existing in a household equipped with several IoT devices, concluding that savings larger than 30% can be obtained, whether on sunny or cloudy days. To the best of our knowledge, these are excellent values compared with existing solutions available in the literature.
Study of Flow and Heat Transfer for the Supercritical Hydrogen in Spallation-Type Cylindrical Neutron Moderator
Authors: Tong Jianfei , Zhu Lingbo , Lu Yiping , Liang Tianjiao , Lu Youlian , Wang Songlin , Yu Chaoju , Dong Shikui , Tan Heping
Published: September 2021

Pipe height in cylindrical neutron moderator is an important factor to flow pattern, temperature distribution and even the neutron characters. In this paper, the steady-state thermal analysis of cold neutron moderator is carrying out with different heights, conjugated heat transfer method and one-way coupled with a neutron transfer software. The different pipe heights, which is the jet-to-surface distances (H/D = 0.5~6), were compared using a 2D moderator model. The results show that vortex size and velocity gradient from container wall to vortex center vary with H/D, the center of recirculation zone nearly remain constant, and heat transfer effect is weakened on the target bottom surface. With H/D increasing, the velocity at bottom target surface is progressively decreased, and cooling effect is poor, leading to the rise in temperature. The optimal range cooling performance is (H/D) = 0.5~1 at Re = 1.7 × 105, and the enhancement of beam power further strengthens the thermal deposition difference between container and liquid hydrogen. The results can be applied to moderator component design and optimization in the future spallation neutron source.
Off-Grid Rural Electrification in India Using Renewable Energy Resources and Different Battery Technologies with a Dynamic Differential Annealed Optimization
Authors: Kumar Polamarasetty P , Suresh Vishnu , Jasinski Michal , Leonowicz Zbigniew
Published: September 2021

Several families in India live in remote places with no access to grid-connected power supply due to their remoteness. The study area chosen from the Indian state of Odisha does not have an electrical power supply due to its distant location. As a result, this study analyzed the electrification process using Renewable Energy (RE) resources available in the locality. However, these RE resources are limited by their dependency on weather conditions and time. So, a robust battery storage system is needed for a continuous power supply. Hence, the Nickel Iron (Ni-Fe), Lithium-Ion (Li-Ion) and Lead Acid (LA) battery technologies have been analyzed to identify a battery technology that is both technologically and economically viable. Using the available RE resources in the study area, such as photovoltaic and biomass energy resources, as well as the various battery technologies, three configurations have been modelled, such as Photovoltaic Panels (PVP)/Biomass Generator(BIOMG)/BATTERY(Ni-Fe), PV/BIOMG/BATTERY(Li-Ion) and PVP/BMG/BATTERY(LA). These three configurations have been examined using nine prominent metaheuristic algorithms, in which the PVP/BIOMG/BATTERY(Ni-Fe) configuration provided the optimal Life Cycle Cost value of 367,586 USD. Among the all metaheuristic algorithms, the dynamic differential annealed optimization algorithm was given the best Life Cycle Cost values for all of the three configurations.
Complex Design Method of Filtration Station Considering Harmonic Components
Authors: Kus Vaclav , Skala Bohumil , Drabek Pavel
Published: September 2021

The paper deals with a new methodology for calculating the filter parameters. The basis is respect for the fact that the real filter current consists of other harmonic components, which filter is tuned. The proposed methodology was used to design filters for traction substation 25 kV/50 Hz. The operation of the locomotives in the AC supply systems of 25 kV/50 Hz leads to a rising of higher order harmonic currents. Due to the 1-phase supply system, these are mainly the 3rd and 5th harmonics. By simulation and subsequent measurement of the proposed traction power station filters the proposed methodology was verified. Thus, the filter design can also be used for filter compensating stations of the standard 3-phase distribution network. The described method presents an optimal filter design without unnecessary oversizing. This fact reduces the size and cost of the filter. It is shown that it is possible to design a filter that meets the requirements for power quality under extreme load and to minimise distortion of line voltage.
Effect of Martensitic Transformation and Grain Misorientation on Surface Roughening Behavior of Stainless Steel Thin Foils
Authors: Aziz Abdul , Yang Ming , Shimizu Tetsuhide , Furushima Tsuyoshi
Published: September 2021

The surface roughening (Ra), martensitic phase transformation (MPT), and grain misorientation (GMO) behavior of stainless steel 304 and 316 in various grain sizes (Dg) were studied experimentally, including five cycles of sequential uniaxial tensile stress testing and Scanning Electron Microscope-Electron Back Scattered Diffraction (SEM-EBSD) investigation. The MPT and GMO characteristics were sequentially investigated using tensile testing and SEM-EBSD analysis. The correlation between MPT, GMO, martensitic volume fraction (Mf), and Ra behavior were investigated. The experimental results showed that increasing the total strain from 5.0% to 25.0% increased the MPT, GMO, and Mf, which were transformed from the metastable austenitic phase in stainless steel (SUS) 304. The increasing total strain increased Ra for all kinds of Dg. Furthermore, SUS 304 and SUS 316 were used to compare the roughening mechanism. The MPT was very high and spread uniformly in fine grain of SUS 304 thin foil, but the MPT was low and not uniform in coarse grain of SUS 304 thin foil. There was no MPT in SUS 316 thin foil, both in coarse and fine grain. The GMO in fine grains, both in SUS 304 and SUS 316 thin foils, spread uniformly. The GMO in coarse grains, both in SUS 304 and SUS 316 thin foils, did not spread uniformly. Surface roughness increased higher in coarse grain than fine grain for both of SUS 304 and SUS 316 thin foil. SUS 304 increased higher than SUS 316 thin foil. The effect of inhomogeneous deformation due to the MPT is a more important factor than GMO in coarse grain.
Cone-Bearing Branches of Pinus koraiensis Are Not Carbon Autonomous during Cone Development
Authors: Wu Haibo , Yin Dongsheng , Salomón Roberto Luis , Rodríguez-Calcerrada Jesús , Zhang Jianying , Zhang Peng , Shen Hailong
Published: September 2021

Cone development in conifer species is crucial to ensure sexual regeneration. A better understanding of carbon (C) source-sink relations at the branch level can guide strategies for improving resource allocation to reproduction. In particular, the evaluation of C relations between vegetative and reproductive branches is helpful to test whether tree branches are carbon autonomous. With this aim, we integrated girdling and defoliation treatments with 13C pulse labeling in situ to evaluate C autonomy in cone-bearing branches of P. koraiensis during the growing season. Girdling significantly reduced branch volumetric development, branch biomass, and non-structural carbohydrates across foliar, twig, and cone tissues; it also arrested cone development. Defoliation effects on these variables were minor, although they tended to increase with defoliation intensity. In addition, 13C increased by 4.5% and 45.4% after 4 h and 24 h of 13C labeling in unlabeled cone-bearing branches, respectively, indicating the C translocation from labeled vegetative branches. These results indicate that the cone-bearing branches are not C autonomous and that the development of female cones relies to a great extent on C import from neighboring branches. However, the amount of C translocated was largely dependent on manipulative alterations of the source-sink balance, thereby denoting extensive plasticity in the degree of branch C autonomy. These results shed light on the reproductive physiology of P. koraiensis.
Preference and Performance of the Pine-Tree Lappet Dendrolimus pini on Various Pine Species
Authors: Łukowski Adrian , Giertych Marian J. , Adamczyk Dawid , Mąderek Ewa , Karolewski Piotr
Published: September 2021

Global commercial and recreational transport may lead to the unintentional invasion of insect species, which in turn may pose a threat to native organisms. In this study, we aimed to assess whether the economically important pest of Pinus sylvestris L., moth Dendrolimus pini L. (DP), is able to feed on nine other pine species, and how this will affect its survival, performance, growth, and development. We carried out food choice tests and a no-choice laboratory feeding experiment. We found that this insect mostly preferred its prime host, but also Pinus cembra L., Pinus contorta Douglas ex Loudon, Pinus nigra J.F.Arnold, and Pinus ponderosa Douglas ex C.Lawson. The performance test revealed a host-specific response of DP to the host plant. This response was manifested in a large variation in body mass as well as in a decrease or increase in life-history traits, such as fecundity, and wing morphology parameters. However, the larvae’s choice of particular hosts corresponded to the results of the performance test. Larvae more willingly selected food allowing better results in their performance. Larvae achieved better values of growth and development when fed on European and North American pine species or on species with two- and three-needle fascicles. In addition, attractants and repellents in needles of different pine species were chemically analyzed. Variations in the secondary metabolite composition as well as the specific leaf area of different pine species effectively explained the results found in the insects, but the content of sugars and nitrogen remains to be elucidated. We speculate that DP poses a serious threat to large areas of pine forests, if transferred, as it can survive and develop on many economically important tree species in North America and Europe.
Durability and Fire Performance of Charred Wood Siding (Shou Sugi Ban)
Authors: Hasburgh Laura E. , Zelinka Samuel L. , Bishell Amy B. , Kirker Grant T.
Published: September 2021

Shou sugi ban, also known as yakisugi, or just sugi ban, is an aesthetic wood surface treatment that involves charring the surface of dimensional lumber, such as exterior cladding. The goal of this research is to examine the effect of shou sugi ban on the flammability and decay resistance of wood. Several species and variants of commercially available sugi ban were tested. The flammability was examined from the heat release rate curves using the oxygen consumption method and cone calorimeter. Durability was examined with a soil block assay for one white-rot fungus and one brown-rot fungus. The testing showed that the shou sugi ban process did not systematically improve the flammability or durability of the siding.
Reader–Tag Commands via Modulation Cutoff Intervals in RFID Systems
Authors: Alma’aitah Abdallah Y. , Massad Mohammad A.
Published: September 2021

Radio frequency identification (RFID) technology facilitates a myriad of applications. In such applications, an efficient reader–tag interrogation process is crucial. Nevertheless, throughout reader–tag communication, significant amounts of time and power are consumed on inescapable simultaneous tag replies (i.e., collisions) due to the lack of carrier sensing at the tags. This paper proposes the modulation cutoff intervals (MCI) process as a novel reader–tag interaction given the lack of carrier sensing constraints in passive RFID tags. MCI is facilitated through a simple digital baseband modulation termination (DBMT) circuit at the tag. DBMT detects the continuous-wave cutoff by the reader. In addition, DBMT provides different flags based on the duration of the continuous-wave cutoff. Given this capability at the tag, the reader cuts off its continuous-wave transmission for predefined intervals to indicate different commands to the interrogated tag(s). The MCI process is applied to tag interrogation (or anti-collision) and tag-counting protocols. The MCI process effect was evaluated by the two protocols under high and low tag populations. The performance of such protocols was significantly enhanced with precise synchronization within time slots with more than 50% and more than 55.6% enhancement on time and power performance of anti-collision and counting protocols, respectively. Through the MCI process, fast and power-efficient tag identification is achieved in inventory systems with low and high tag mobility; alternatively, in addition to the rapid and power efficient interaction with tags, anonymous tag counting is conducted by the proposed process.
Food on the Move: The Impact of Implied Motion in Pictures on Food Perceptions through Anticipated Pleasure of Consumption
Authors: Mulier Lana , Meersseman Eva , Vermeir Iris , Slabbinck Hendrik
Published: September 2021

To tackle obesity, upgrading the image of healthy food is increasingly relevant. Rather than focusing on long-term benefits, an effective way to promote healthy food consumption through visual advertising is to increase its pleasure perception. We investigate whether implied motion, a popular trend in food pictures, affects food perceptions through anticipated consumption pleasure. Prior research shows that motion affects food perceptions, but these studies focused on limited food categories, using experiments with a single food stimulus, and mainly showing unhealthy food effects. Therefore, we aim to (1) replicate prior findings on the effects of food in motion on appeal, tastiness, healthiness, and freshness perceptions; (2) examine whether these effects differ for healthy and unhealthy food; and (3) investigate whether anticipated pleasure of consumption drives the effects of implied motion on food perceptions. Three between-subjects experiments (N = 626) reveal no evidence for the effectiveness of motion (vs. no motion) across a large variety of food products. We further show no differential effects for healthy versus unhealthy foods. Moreover, implied motion does not increase appeal or taste perceptions through anticipated pleasure. Considering the current replication crisis, these findings provide more nuanced insights into the effectiveness of motion in visual food advertising.
Polycyclic Aromatic Hydrocarbon Risk Assessment and Analytical Methods Using QuEchERS Pretreatment for the Evaluation of Herbal Medicine Ingredients in Korea
Authors: Hwang Hee-Jeong , Lee Sae-Ha , Kim Yong-Yeon , Shin Han-Seung
Published: September 2021

Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic and mutagenic compounds that are often formed during the thermal processing of herbal medicine ingredients. In this study, the concentrations of four PAHs (PAH4) in various herbal medicine ingredients were monitored. Further, the QuEChERS method was used to replace conventional pretreatment, a more complex and cumbersome approach. The recovery range of the QuEChERS method ranged between 89.65–118.59%, and the average detection levels of benzo[a]anthracene (BaA), chrysene (CHR), benzo[b]fluoranthene (BbF), and Benzo[a]pyrene (BaP) in 50 herbal medicine ingredients were 0.18, 0.27, 1.13, and 0.17 μg/kg, respectively. The BaP and PAH4 levels in all tested samples were deemed safe according to risk characterization analyses based on European Union and Korean guidelines. Therefore, our findings indicated that the QuEChERS method could be used as an effective alternative to conventional sample pretreatment for the analysis of herbal medicine ingredients.
Thermophysical Investigation of Oldroyd-B Fluid with Functional Effects of Permeability: Memory Effect Study Using Non-Singular Kernel Derivative Approach
Authors: Riaz Muhammad Bilal , Awrejcewicz Jan , Rehman Aziz-Ur , Akgül Ali
Published: September 2021

It is well established fact that the functional effects, such as relaxation and retardation of materials, can be measured for magnetized permeability based on relative increase or decrease during magnetization. In this context, a mathematical model is formulated based on slippage and non-slippage assumptions for Oldroyd-B fluid with magnetized permeability. An innovative definition of Caputo-Fabrizio time fractional derivative is implemented to hypothesize the constitutive energy and momentum equations. The exact solutions of presented problem, are determined by using mathematical techniques, namely Laplace transform with slipping boundary conditions have been invoked to tackle governing equations of velocity and temperature. The Nusselt number and limiting solutions have also been persuaded to estimate the heat emission rate through physical interpretation. In order to provide the validation of the problem, the absence of retardation time parameter led the investigated solutions with good agreement in literature. Additionally, comprehensively scrutinize the dynamics of the considered problem with parametric analysis is accomplished, the graphical illustration is depicted for slipping and non-slipping solutions for temperature and velocity. A comparative studies between fractional and non-fractional models describes that the fractional model elucidate the memory effects more efficiently.
A Curious Novel Combination of Nucleophosmin (NPM1) Gene Mutations Leading to Aberrant Cytoplasmic Dislocation of NPM1 in Acute Myeloid Leukemia (AML)
Authors: Venanzi Alessandra , Rossi Roberta , Martino Giovanni , Annibali Ombretta , Avvisati Giuseppe , Mameli Maria Grazia , Sportoletti Paolo , Tiacci Enrico , Falini Brunangelo , Martelli Maria Paola
Published: September 2021

Nucleophosmin (NPM1) mutations occurring in acute myeloid leukemia (AML) (about 50 so far identified) cluster almost exclusively in exon 12 and lead to common changes at the NPM1 mutants C-terminus, i.e., loss of tryptophans 288 and 290 (or 290 alone) and creation of a new nuclear export signal (NES), at the bases of exportin-1(XPO1)-mediated aberrant cytoplasmic NPM1. Immunohistochemistry (IHC) detects cytoplasmic NPM1 and is predictive of the molecular alteration. Besides IHC and molecular sequencing, Western blotting (WB) with anti-NPM1 mutant specific antibodies is another approach to identify NPM1-mutated AML. Here, we show that among 382 AML cases with NPM1 exon 12 mutations, one was not recognized by WB, and describe the discovery of a novel combination of two mutations involving exon 12. This appeared as a conventional mutation A with the known TCTG nucleotides insertion/duplication accompanied by a second event (i.e., an 8-nucleotide deletion occurring 15 nucleotides downstream of the TCTG insertion), resulting in a new C-terminal protein sequence. Strikingly, the sequence included a functional NES ensuring cytoplasmic relocation of the new mutant supporting the role of cytoplasmic NPM1 as critical in AML leukemogenesis.
Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice
Authors: Anaclerio Federico , Ferrante Rossella , Mandatori Domitilla , Antonucci Ivana , Capanna Matteo , Damiani Verena , Tomo Pamela Di , Ferrante Roberto , Ranaudo Marianna , De Laurenzi Vincenzo , Stuppia Liborio , De Fabritiis Simone
Published: September 2021

A considerable effort has been devoted in all countries to react to the COVID-19 pandemic by tracing infected individuals, containing the spread of the disease, identifying therapies, and producing and distributing vaccines. Currently, a significant concern is the appearance of variants of the virus that may frustrate these efforts by showing increased transmissibility, increased disease severity, reduced response to therapy or vaccines, and ability to escape diagnosis. All countries have therefore devoted a massive attempt to the identification and tracking of these variants, which requires a vast technological effort to sequence a large number of viral genomes. In this paper, we report our experience as one of the Italian laboratories involved in SARS-CoV-2 variant tracing. We summarize the different approaches used, and outline a potential model combining several techniques to increase tracing ability while at the same time minimizing costs.
The Role of Drosophila CtIP in Homology-Directed Repair of DNA Double-Strand Breaks
Authors: Yannuzzi Ian , Butler Margaret A. , Fernandez Joel , LaRocque Jeannine R.
Published: September 2021

DNA double-strand breaks (DSBs) are a particularly genotoxic type of DNA damage that can result in chromosomal aberrations. Thus, proper repair of DSBs is essential to maintaining genome integrity. DSBs can be repaired by non-homologous end joining (NHEJ), where ends are processed before joining through ligation. Alternatively, DSBs can be repaired through homology-directed repair, either by homologous recombination (HR) or single-strand annealing (SSA). Both types of homology-directed repair are initiated by DNA end resection. In cultured human cells, the protein CtIP has been shown to play a role in DNA end resection through its interactions with CDK, BRCA1, DNA2, and the MRN complex. To elucidate the role of CtIP in a multicellular context, CRISPR/Cas9 genome editing was used to create a DmCtIPΔ allele in Drosophila melanogaster. Using the DSB repair reporter assay direct repeat of white (DR-white), a two-fold decrease in HR in DmCtIPΔ/Δ mutants was observed when compared to heterozygous controls. However, analysis of HR gene conversion tracts (GCTs) suggests DmCtIP plays a minimal role in determining GCT length. To assess the function of DmCtIP on both short (~550 bp) and long (~3.6 kb) end resection, modified homology-directed SSA repair assays were implemented, resulting in a two-fold decrease in SSA repair in both short and extensive end resection requirements in the DmCtIPΔ/Δ mutants compared to heterozygote controls. Through these analyses, we affirmed the importance of end resection on DSB repair pathway choice in multicellular systems, described the function of DmCtIP in short and extensive DNA end resection, and determined the impact of end resection on GCT length during HR.
Cosmogenic Exposure Dating (36Cl) of Landforms on Jan Mayen, North Atlantic, and the Effects of Bedrock Formation Age Assumptions on 36Cl Ages
Authors: Anjar Johanna , Akҫar Naki , Larsen Eiliv A. , Lyså Astrid , Marrero Shasta , Mozafari Nasim , Vockenhuber Christof
Published: September 2021

Jan Mayen is a small volcanic island situated 550 km north of Iceland. Glacial sediments and landforms are relatively common on the island but, so far, only a few of them have been dated. In this study, we present and discuss 89 36Cl dates of primarily glacial and volcanic events on Jan Mayen. Calculations of sample exposure ages were complicated by young exposure ages, young rock formation age, and high native Cl contents, leading to updates in CRONUScalc to enable accurate exposure age calculations. The samples provide good evidence against an equilibrium assumption when subtracting background production (e.g., 36Cl produced by neutron capture from fission of U or Th) for samples on young bedrock, with younger exposure ages most significantly affected. Exposure ages were calculated with a range of assumptions of bedrock formation ages appropriate for Jan Mayen, including the assumption that the rock formation age equaled the exposure age (i.e., the youngest age it could possibly have), and we found that although the effect on most of the ages was small, the calculated ages of 25 of the samples increased by more than 1 standard deviation from the age calculated assuming equilibrium background production, with a maximum deviation of 6.1 ka. Due to the very young bedrock on Jan Mayen, we consider the nonequilibrium ages to be the most reliable ages from the island and conclude that large-scale deglaciation on the south and central, lower-lying, parts of the island, started around 20 ka and lasted until ~7 ka. On northern Jan Mayen, the slopes of the 2277 m high stratovolcano Beerenberg are currently partly glaciated; however, outside of the Little Ice Age moraines, all but two samples give ages between 14 and 5.7 ka.
Optimizing Seed Germination and Seedling Growth in Different Kiwifruit Genotypes
Authors: Maghdouri Maryam , Ghasemnezhad Mahmood , Rabiei Babak , Golmohammadi Morteza , Atak Arif
Published: September 2021

The presence of dormant embryos in seeds and nonuniformity in seedling growth are the main limiting factors for kiwifruit propagation. Studies on the germination of hybrid genotypes are limited, especially at different ploidy levels. Low germination percentages and nonuniformity in seedling growth are two of the limiting factors of kiwifruit breeding, especially new and imported germplasm. The effect of cold moist stratification and growing substances on seed germination and seedling growth, respectively, on different kiwifruit seed populations were evaluated in this study. The effect of cold moist stratification (3, 4 and 5 weeks at 4 °C) and growing substrate composition (peat, perlite and coco peat) on seed germination and seedling growth of different Actinidia genotypes were assessed. In general, increasing stratification period duration often increased germination speed and uniformity. It was also found that increasing the ratio of peat moss improved the vegetative growth parameters of all kiwifruit seedlings. Stratification treatment, light and temperature fluctuation and consequently suitable substrate can accelerate and increase the production of seedlings and reduce seedling losses. It was also found that peat–perlite (1:1) medium was the best medium for kiwifruit seedling growth.
Can the Caper (Capparis spinosa L.) Still Be Considered a Difficult-to-Propagate Crop?
Authors: Sottile Francesco , Caltagirone Chiara , Peano Cristiana , Del Signore Maria Beatrice , Barone Ettore
Published: September 2021

As a perennial xerophytic shrub, characterized by plesiomorphic features, the caper (Capparis spinosa L.) is naturally spread throughout the Mediterranean basin and occupies an important ecological role, as well as an economic one, in traditional and specialized systems for commercial production. This species, in spite of its wide diffusion, is currently considered at risk of genetic erosion, mainly due to overgrazing and overharvesting for domestic uses and for trade. This situation is made more serious because of the lack of efficient propagation techniques, determining the caper as a “difficult-to-propagate species”. In this review, we report the main available sexual and vegetative propagation techniques with the aim of assessing whether, and to what extent, this criticality is still true for caper as a horticultural crop. In terms of seed propagation, germination rates have generally been considered quite low or unsatisfactory, and are also affected by hybridization phenomena that are likely to occur among both the wild and cultivated forms. The seeds show a physiological dormancy that can be lowered by adopting hormonal treatments, but in situ germination remains a critical phase. Vegetative propagation appears quite effective, mostly as related to in vitro techniques that allow caper cultivation that is no longer affected by propagation for an economic dissemination of the species in more intensive orchards. The research needs for Caper spinosa L. as a horticultural crop, especially in the field of genetic improvement and breeding, are also underlined.
A Reliable Regeneration Method in Genome-Editable Bell Pepper ‘Dempsey’
Authors: Won Kang-Hee , Park Sung-il , Choi Jisun , Kim Hyun Hee , Kang Byoung-Cheorl , Kim Hyeran
Published: September 2021

A reliable regeneration technique is critical for the improvement of pepper traits in the genome editing era. Recently, we reported that peppers were successfully and specifically edited using CRISPR tools, CRISPR/Cas9 and CRISPR/Cas12a (LbCpf1). Although genome-editing tools can be applied to modify peppers at the cellular level, feasible pepper regeneration techniques have not been developed. Therefore, we studied a pepper regeneration protocol for Capsicum annuum L. ‘Dempsey’, a bell pepper species that has been proven to be genome-editable. Three explant types were used in this study, including the first leaves, cotyledons and hypocotyls of pepper seedlings. The shoot buds of the tested explants were produced using 8 mg/L 6-benzylaminopurine (BAP)- and 6 mg/L indole-3-acetic acid (IAA)-containing shoot induction medium (SIM). The first leaves of the ‘Dempsey’ seedlings showed an average shooting rate of 69.8%, whereas the hypocotyls and cotyledons had approximately 25.5% and 19.5% shooting rates, respectively. The regenerated ‘Dempsey’ plants exhibited no alterations in fruit and fertile seed phenotypes. Furthermore, the parent ‘Dempsey’ and progenies of the regenerants were cytogenetically stable with the same chromosome numbers (2n = 24). Therefore, this regeneration protocol enables the precise molecular breeding of ‘Dempsey’ peppers when coupled with CRISPR tools.
Physicochemical Changes, Peel Colour, and Juice Attributes of Blood Orange Cultivars Stored at Different Temperatures
Authors: Habibi Fariborz , Guillén Fabián , Serrano María , Valero Daniel
Published: September 2021

Changes in physicochemical traits, peel colour, and juice attributes of four blood orange cultivars (‘Moro’, ‘Tarocco’, ‘Sanguinello’, and ‘Sanguine’) were evaluated during 180 days at 2 and 5 °C plus 2 days at 20 °C for shelf life. ‘Tarocco’ had the lowest weight and firmness losses at both temperatures during storage. Titratable acidity (TA) at 5 °C was higher than 2 °C, with ‘Sanguinello’ and ‘Tarocco’ showing the highest and lowest TA, respectively. Juice content decreased during storage at both temperatures, although ‘Sanguinello’ had the highest juice content among the tested cultivars. Peel colour parameters including L* (lightness), b*, hue angle (h°), and chroma (C*) decreased during cold storage, while a* and citrus colour index (CCI) increased in all cultivars at both temperatures. The order for CCI was ‘Tarocco’ > ‘Moro’ > ‘Sanguinello’ > ‘Sanguine’. Overall, prolonged storage at 5 °C was considered as optimum temperature for all cultivars, although ‘Sanguinello’ cultivar had a better aptitude for the citrus juice industry.
Tuberculosis Death Epidemiology and Its Associated Risk Factors in Sabah, Malaysia
Authors: Avoi Richard , Liaw Yau Chun
Published: September 2021

Tuberculosis (TB) is a leading killer from a single infectious agent globally. In 2019, Malaysia’s TB incidence rate was 92 per 100,000 population, and the TB mortality rate was estimated at 4 cases per 100,000 population per year. However, the state of Sabah had a higher burden of TB with a notification rate of 128 per 100,000 population and a TB case fatality rate of 8% compared to the national figure. This study aims to provide a comprehensive report on TB deaths epidemiology and its associated factors at a sub-national level. This nested case-control study used Sabah State Health Department TB surveillance data from the Malaysia national case-based TB registry (MyTB) between 2014 and 2018. Cases were defined as all-cause TB deaths that occurred before anti-TB treatment completion from the time of TB diagnosis. Controls were randomly selected from TB patients who completed anti-TB treatment. The TB mortality rate had increased significantly from 9.0/100,000 population in 2014 to 11.4/100,000 population in 2018. The majority of TB deaths occurred in the first two months of treatment. TB-related deaths were primarily due to advanced disease or disseminated TB, whereas non-TB-related deaths were primarily due to existing comorbidities. Many important independent risk factors for TB deaths were identified which are useful to address the increasing TB mortality rate.