bluuebunny/crossref_metadata_2025 huggingface dataset:
Topography, Diversity, and Forest Structure Attributes Drive Aboveground Carbon Storage in Different Forest Types in Northeast China
Authors: Jia Bo , Guo Weiwei , He Jingyuan , Sun Minggang , Chai Lei , Liu Jiarong , Wang Xinjie
Published: March 2022

Forests regulate air quality and respond to climate change by storing carbon. Assessing the driving factors of forest aboveground carbon (AGC) storage is of great importance for forest management. We assumed that different forest types would affect the relationship between species richness, stand density, individual tree size variation, and AGC. In order to test and verify it, we analyzed the inventory data of 206 fixed plots (20 m × 20 m) of Jingouling Forest Farm, taking advantage of the piecewise structural equation model (pSEM) to explore the effects of species diversity, stand structure attributes, and topography on the AGC storage in the Wangqing Forest in Jilin Province. In addition, in this study, we aimed to investigate whether the fixed factors (species diversity, stand structure attributes, and topography) influenced AGC storage more significantly than the random factor (forest type). According to the results of pSEM, the selected factors jointly explain the impact on 33% of AGC storage. The relationship between stand density and AGC is positive, and the impact of individual tree size variation on AGC storage is negative. Species richness has direct and indirect impacts on AGC storage, and the indirect impact is more significant through individual tree size variation. Both elevation and slope are significantly negatively associated with AGC storage. Forest type explains the impact on 12% of AGC storage, which means the relationship between AGC and predictors varies across forest types. The results provide a scientific basis for the protection and management decision of natural forests in northeastern China.
CPU-GPU-Memory DVFS for Power-Efficient MPSoC in Mobile Cyber Physical Systems
Authors: Dey Somdip , Isuwa Samuel , Saha Suman , Singh Amit Kumar , McDonald-Maier Klaus
Published: March 2022

Most modern mobile cyber-physical systems such as smartphones come equipped with multi-processor systems-on-chip (MPSoCs) with variant computing capacity both to cater to performance requirements and reduce power consumption when executing an application. In this paper, we propose a novel approach to dynamic voltage and frequency scaling (DVFS) on CPU, GPU and RAM in a mobile MPSoC, which caters to the performance requirements of the executing application while consuming low power. We evaluate our methodology on a real hardware platform, Odroid XU4, and the experimental results prove the approach to be 26% more power-efficient and 21% more thermal-efficient compared to the state-of-the-art system.
Application of Antibody and Immunoassay for Food Safety
Authors: Lei Hongtao , Wang Zhanhui , Eremin Sergei A. , Liu Zhiwei
Published: March 2022

This Special Issue of Foods, Application of Antibody and Immunoassay for Food Safety, contains ten papers that were refereed and selected in accordance with the usual editorial standards of the journal [...]
Consumers’ Perception and Preference for the Consumption of Wild Game Meat among Adults in Poland
Authors: Czarniecka-Skubina Ewa , Stasiak Dariusz M. , Latoch Agnieszka , Owczarek Tomasz , Hamulka Jadwiga
Published: March 2022

Wild game meat can be a healthier, safer, and more environmentally friendly alternative to meat from farm animals. The aims of this study were to know the preferences and opinions of Polish consumers regarding game meat and its use in their diet, and to identify consumer segments based on differences in individual game meat choices, concerns, and eating habits related to game meat. The survey was conducted using the platform for online surveys among 1261 adult Poles. Six clusters characterizing the behavior of game consumers were identified (casual consumers, occasional game gourmets, indifferent consumers, occasional consumers, accidental consumers, wild game lovers) and four clusters among those who do not eat game (uninterested, restricted, dislikers, fearful). It has been found that wild game is more often eaten by hunters and their family or friends. The most common reasons for not consuming game are high prices, low availability, no family tradition, and unacceptable taste. Many positive respondents eat game because of its nutritional value but are concerned about the potential health risks and lack of cooking skills. The results of this study indicate the need for information programs for consumers about this meat. They will provide guidance to meat companies about consumer preferences for game and allow them to develop appropriate marketing strategies.
Natural Hydrogel-Based Bio-Inks for 3D Bioprinting in Tissue Engineering: A Review
Authors: Fatimi Ahmed , Okoro Oseweuba Valentine , Podstawczyk Daria , Siminska-Stanny Julia , Shavandi Amin
Published: March 2022

Three-dimensional (3D) printing is well acknowledged to constitute an important technology in tissue engineering, largely due to the increasing global demand for organ replacement and tissue regeneration. In 3D bioprinting, which is a step ahead of 3D biomaterial printing, the ink employed is impregnated with cells, without compromising ink printability. This allows for immediate scaffold cellularization and generation of complex structures. The use of cell-laden inks or bio-inks provides the opportunity for enhanced cell differentiation for organ fabrication and regeneration. Recognizing the importance of such bio-inks, the current study comprehensively explores the state of the art of the utilization of bio-inks based on natural polymers (biopolymers), such as cellulose, agarose, alginate, decellularized matrix, in 3D bioprinting. Discussions regarding progress in bioprinting, techniques and approaches employed in the bioprinting of natural polymers, and limitations and prospects concerning future trends in human-scale tissue and organ fabrication are also presented.
The Role of NEDD4 E3 Ubiquitin–Protein Ligases in Parkinson’s Disease
Authors: Conway James A. , Kinsman Grant , Kramer Edgar R.
Published: March 2022

Parkinson’s disease (PD) is a debilitating neurodegenerative disease that causes a great clinical burden. However, its exact molecular pathologies are not fully understood. Whilst there are a number of avenues for research into slowing, halting, or reversing PD, one central idea is to enhance the clearance of the proposed aetiological protein, oligomeric α-synuclein. Oligomeric α-synuclein is the main constituent protein in Lewy bodies and neurites and is considered neurotoxic. Multiple E3 ubiquitin-protein ligases, including the NEDD4 (neural precursor cell expressed developmentally downregulated protein 4) family, parkin, SIAH (mammalian homologues of Drosophila seven in absentia), CHIP (carboxy-terminus of Hsc70 interacting protein), and SCFFXBL5 SCF ubiquitin ligase assembled by the S-phase kinase-associated protein (SKP1), cullin-1 (Cul1), a zinc-binding RING finger protein, and the F-box domain/Leucine-rich repeat protein 5-containing protein FBXL5), have been shown to be able to ubiquitinate α-synuclein, influencing its subsequent degradation via the proteasome or lysosome. Here, we explore the link between NEDD4 ligases and PD, which is not only via α-synuclein but further strengthened by several additional substrates and interaction partners. Some members of the NEDD4 family of ligases are thought to crosstalk even with PD-related genes and proteins found to be mutated in familial forms of PD. Mutations in NEDD4 family genes have not been observed in PD patients, most likely because of their essential survival function during development. Following further in vivo studies, it has been thought that NEDD4 ligases may be viable therapeutic targets in PD. NEDD4 family members could clear toxic proteins, enhancing cell survival and slowing disease progression, or might diminish beneficial proteins, reducing cell survival and accelerating disease progression. Here, we review studies to date on the expression and function of NEDD4 ubiquitin ligases in the brain and their possible impact on PD pathology.
Black Children’s Lives Matter: Representational Violence against Black Children
Authors: Lester Neal A.
Published: March 2022

Black children have never been exempt from the violence and abuse that have beset Black adults. Any comprehensive attention to and understanding of systemic racism, anti-Blackness, and intergenerational Black trauma must consider the historical violence literally, representationally, and fictionally against Black children and youth. For each news story headline about violence against Black children, there is a comparable Black adult story, underscoring the interchangeability of Black adult and Black children subjected to racial violence. This essay is not a history of violence against Black children in literature but, rather, an effort to understand and demonstrate that Black children’s lives have not always mattered and that to address true racial justice in this country, systemic assaults on Black children and, by extension, on Black children’s families and communities, must be included in any justice conversation and work. This essay looks at representative children’s literature that normalizes violence against Black children.
Patient Active Approaches in Osteopathic Practice: A Scoping Review
Authors: Lunghi Christian , Baroni Francesca , Amodio Andrea , Consorti Giacomo , Tramontano Marco , Liem Torsten
Published: March 2022

Background: In the field of manual therapies there is a growing interest in moving from passive hands-on approaches to patient active approaches. In the osteopathic field there are both active and passive methods described as integrated in the process of care. However, this prospective linkage has not been formally explored and is not well shared in the community of practice. The present review aims to appraise the relevant literature on the functioning and principles of Patient active osteopathic approaches (PAOAs) and explore a prospective model for selecting the different types of PAOA, highlighting their integration into patient management strategies. Methods: A scoping review was conducted to analyze the relevant literature on the functioning and the different principles of PAOA and to obtain a comprehensive perspective on the phenomenon. Results: The eligible articles provide insights into the mechanisms of functioning and principles of application of active approaches to be integrated with hands-on approaches. These results provide new insights into the relevance of PAOA to clinical practice. Conclusions: The proposal, emerging from the review, may promote discussions in the community of practice and provide a road map for research towards achieving an evidence-based structure for PAOA.
Two-Month Individually Supervised Exercise Therapy Improves Walking Speed, Step Length, and Temporal Gait Symmetry in Chronic Stroke Patients: A before–after Trial
Authors: Yoshioka Kiyoshi , Watanabe Tatsunori , Maruyama Norikazu , Yoshioka Mizuki , Iino Keita , Honda Kimikazu , Hayashida Koshiro
Published: March 2022

Gait asymmetry is common after stroke and is a major risk factor for falls. In particular, temporal gait asymmetry often remains in the chronic stage of stroke. However, health insurance does not cover rehabilitation for patients with chronic stroke in many countries. Accordingly, it is undetermined whether individually supervised exercise therapy has beneficial effects on chronic hemiparetic gait. Patients with stroke (n = 25) more than 6 months after onset performed 70 min of individually supervised exercise twice weekly for 2 months in 16 sessions with qualified personnel. The intervention significantly reduced the pre-swing phase on the paretic side (mean = 91.8%, 95%CI, 84.8–98.8). In addition, there was a significant improvement in pre-swing phase symmetry in those with great asymmetry prior to the intervention (p = 0.022). Step length significantly increased after the intervention on both sides (non-paretic, p = 0.029; paretic, p = 0.0055). Walking time at both comfortable and maximum speeds was significantly shortened (comfortable, p = 0.0041; maximum, p < 0.0001). Our findings suggest that there remains scope to improve gait ability with individually supervised exercise therapy in patients with chronic stroke, whose functional recovery is often considered unlikely. This type of intervention may be a simple and effective option to improve gait parameters, including temporal asymmetry, even in patients with chronic stroke.
The Optimization of HIV Testing in Eastern Indonesia: Findings from the 2017 Indonesian Demographic and Health Survey
Authors: Ibrahim Kusman , Arifin Hidayat , Fitri Siti Ulfah Rifa’atul , Herliani Yusshy Kurnia , Harun Hasniatisari , Setiawan Agus , Lee Bih-O
Published: March 2022

There is a concerning increase in human immunodeficiency virus (HIV) incidence in eastern Indonesia. HIV testing rates in this area are the lowest in the country. This study aimed to analyze the determinants of HIV testing in the Public Health Centers (PHCs) in eastern Indonesia. A cross-sectional study design was utilized using secondary data from the 2017 Indonesian Demographic and Health Survey (IDHS). We focused the survey on eastern Indonesia (Sulawesi, Maluku, and Papua) with PHC settings. After we weighted and removed missing data, we obtained 2118 surveys (425 males and 1693 females aged 14–54 years). Chi-square and binary logistic regression were used to analyze the determinants of HIV testing optimization in eastern Indonesia with a significance level of p < 0.05. The HIV testing coverage at the PHCs in eastern Indonesia was found to be 28.28%. From the survey, we found that respondents from the province of Papua (AOR: 1.45; 95% CI: 1.09–1.91), those who were female (AOR: 2.37; 95% CI: 1.75–3.12), and those with more information on HIV (AOR: 1.88; 95% CI: 1.41–2.51) were more likely to undergo HIV testing at the PHCs. Meanwhile, the wealthiest respondents (AOR: 0.61; 95% CI: 0.42–0.89) and the respondents who engaged in the perpetuation of stigmatization (AOR: 0.65; 95% CI: 0.42–1.02) were less likely to undergo HIV testing at the PHCs. The coverage of HIV testing in eastern Indonesia needs special attention from the Indonesian government. Increasing equity, distributing information regarding HIV and acquired immunodeficiency syndrome (AIDS) through social media, and creating accessible HIV testing in rural areas are vital for developing appropriate interventions.
Comparison of Aroma Trait of the White-Fleshed Peach ‘Hu Jing Mi Lu’ and the Yellow-Fleshed Peach ‘Jin Yuan’ Based on Odor Activity Value and Odor Characteristics
Authors: Liu Wenjing , Zhang Yuanyuan , Ma Ruijuan , Yu Mingliang
Published: March 2022

Peach flesh colors and aromas impact greatly on consumer behaviors and these two traits are closely associated in white- and yellow-fleshed peaches. However, current understanding of their aromas is rather limited and confined to the concentration differences of some volatiles. Therefore, this study aims to compare the overall aromas of the white-fleshed peach ‘Hu Jing Mi Lu’ (HJML) and yellow-fleshed peach ‘Jin Yuan’ (JY), two fresh cultivars with intense aromas and industrial influence by applications such as HS-SPME/GC-MS analysis, odor activity value evaluations, and odor note analysis. The significant contributions of 26 odor-active compounds to their aromas were revealed. Among which, 15 compounds showed no concentration differences and contributed to the fruity, floral, sweet, etc., odors in both HJML and JY; (E)-2-nonenal, 1-pentanol, and styrene showed significantly higher concentrations in HJML and conveyed much stronger fusel-like and balsamic odors; likewise, (Z)-3-hexenyl acetate, octanal, nonanal, and 3,5-octadien-2-one showed significantly higher concentrations in JY and conveyed much stronger banana, citrus-like, and honey odors; besides, benzyl alcohol, 1-heptanol, 1-octen-3-ol, and 3-octanone with woody, earthy, mushroom, and lavender odors were exclusively detected in HJML. Overall, apart from the common and stronger specific odors in either the white- or yellow-fleshed peach cultivar, the white-fleshed peach was endowed with a unique aroma.
MicroRNA-21 Expression as a Prognostic Biomarker in Oral Cancer: Systematic Review and Meta-Analysis
Authors: Dioguardi Mario , Spirito Francesca , Sovereto Diego , Alovisi Mario , Troiano Giuseppe , Aiuto Riccardo , Garcovich Daniele , Crincoli Vito , Laino Luigi , Cazzolla Angela Pia , Caloro Giorgia Apollonia , Di Cosola Michele , Lo Muzio Lorenzo
Published: March 2022

Oral carcinoma represents one of the main carcinomas of the head and neck region, with a 5-year survival rate of less than 50%. Smoking and tobacco use are recognized risk factors. Prognostic survival biomarkers can be a valid tool for assessing a patient’s life expectancy and directing therapy towards specific targets. Among the biomarkers, the alteration of miR-21 expression in tumor tissues is increasingly reported as a valid prognostic biomarker of survival for oral cancer. The purpose of this meta-analysis was, therefore, to investigate and summarize the results in the literature concerning the potential prognostic expression of tissue miR-21 in patients with OSCC. Methods: The systematic review was conducted following the PRISMA guidelines using electronic databases, such as PubMed, Scopus, and the Cochrane Central Register of Controlled Trials, with the use of combinations of keywords, such as miR-21 AND oral cancer, microRNA AND oral cancer, and miR-21. The meta-analysis was performed using the RevMan 5.41 software. Results: At the end of the article-selection process, 10 studies were included in the meta-analysis, and the result for the main outcome was a pooled HR per overall survival (OS) of 1.29 (1.16–1.44) between high and low expression of miR-21. Conclusions: The data in the literature and the results emerging from the systematic review indicate that miR-21 can provide a prognostic indication in oral cancer.
Continuous VOCs Monitoring in Saturated and Unsaturated Zones Using Thermal Desorber and Gas Chromatography: System Development and Field Application
Authors: An Jinsung , Baek Dong-Jun , Hong Jiseok , Choi Eunsoo , Kim Ijung
Published: March 2022

Subsurface VOC monitoring has been mainly based on manual sampling, transport, and analysis, which would require a sufficient amount of samples to ensure data accuracy and reliability, and additional costs to ensure sample quality. Therefore, a continuous on-site monitoring system is desirable for accurate measurement and subsequent risk assessment. In this study, benzene, toluene, ethylbenzene, and xylene (BTEX) were continuously monitored by the system based on a thermal desorber (TD) and gas chromatography (GC) in an oil-contaminated site that consisted of saturated and unsaturated zones. For the saturated zone, fully automated groundwater sampling and purging processes were performed, and the gasified samples were applied to the TD–GC system. For the unsaturated zone, the gaseous sample in the site was directly applied to the TD–GC system. After verifying the accuracy and precision of the monitoring system, the continuous monitoring system was successfully operated for more than a month in the field. The monitoring system used in this study is applicable to other sites for continuous monitoring, thus providing a scientific background for advanced risk assessment and policy development.
“Yeah, I Mean, You’re Going to Handball, so You Want to Use Balls as Much as Possible at Training”: End-Users’ Perspectives of Injury Prevention Training for Youth Handball Players
Authors: Moesch Karin , Bunke Sofia , Linnéll Jennie , Brodin Eva M. , Donaldson Alex , Ageberg Eva
Published: March 2022

Young handball players experience high injury rates. Specific injury prevention programs reduce injury rates but are not well implemented into youth players’ training. The ‘Implementing injury Prevention training ROutines in TEams and Clubs in youth Team handball (I-PROTECT)’ project addresses this challenge. The aim of this study was to investigate how youth handball coaches and players experienced the recently developed I-PROTECT GO pilot program, by focusing on barriers and facilitators. Three focus group interviews were conducted with coaches and players, and their answers were analyzed using a general inductive approach. The participants appreciated the program and found it useful for their context. The participants’ statements about facilitators and barriers centered around the categories of resources, exercises, program design, and learning. Facilitators that emerged were motivating exercises (e.g., handball-specific), a helpful set-up (e.g., variation), having a clear purpose of exercises, the possibility to fulfil basic psychological needs while training, receiving instructions and feedback, and having role models. Barriers that emerged were limited space and material, difficulties with exercises, an unhelpful set-up (e.g., too repetitive), and undisciplined training. It is important to address perceived barriers and facilitators among coaches and players when developing injury prevention training programs to enhance the uptake of such training.
Are We Still a Sexist Society? Primary Socialisation and Adherence to Gender Roles in Childhood
Authors: Cerbara Loredana , Ciancimino Giulia , Tintori Antonio
Published: March 2022

Background: The internalisation of gender stereotypes has long-term impacts on the aspirations, opportunities and psychosocial well-being of people. The main objective of this study is to measure the adherence to gender roles among children, analysing the link between their roles’ internalisation, the family context and the socioeconomic environment. Method: During the Spring 2021, a survey was carried in Rome on children aged 8–11 through a structured questionnaire. The explanatory dimensions of the analysed topics were identified and a survey questionnaire with an ad hoc administration method were developed. Results: The results show a widespread internalisation of traditional gender roles among the respondents and differences by sex were found, since their acceptance is higher among boys for male roles and among girls for female roles. As the age increases, the adherence to male roles decreases for both boys and girls, while high levels of prosociality resulted in a lower adherence to female roles among boys. No significant relations were found with family and environmental variables. Conclusions: These findings show how the internalisation of gender stereotypes is already traceable at this age, and due to a different path of primary socialisation, boys and girls develop their gender identity consistent with social expectations. The lack of significant relations with environmental variables could be related to the age of the respondents, as the process of primary socialisation imbued with gender stereotypes still does not overlap secondary socialisation. These trends should be monitored during late childhood since at this age children are cognitively plastic, but also vulnerable and influenceable by surrounding stimuli. This research approach, especially if extended to a wider geographical scale, can provide important knowledge to support the relational well-being of children and equal opportunities of society as a whole.
A Very Rare Basidiobolomycosis Case Presented with Cecal Perforation and Concomitant Hepatic Involvement in an Elderly Male Patient: A Case Study
Authors: Abduh Maisa S. , Aldaqal Saleh M. , Almaghrabi Jaudah , Aljiffry Murad M. , Elbadrawy Hany A. , Alsahafi Majid A.
Published: March 2022

This is a case report of Basidiobolomycosis in a 65-year-old male patient from Jizan presenting with colonic perforation and concomitant liver involvement from February 2021 to July 2021. To control the infection, the patient underwent colonic resection and segmental liver resection, as well as three antifungal drugs. The treatment was successful, and the condition was completely resolved.
Health, Psychological and Demographic Predictors of Depression in People with Fibromyalgia and Osteoarthritis
Authors: Van Dyne Angelina , Moy Jason , Wash Kalila , Thompson Linda , Skow Taylor , Roesch Scott C. , Cronan Terry
Published: March 2022

Depression is common in people with fibromyalgia (FM) and osteoarthritis (OA) and has been linked to adverse health outcomes in these conditions. The purpose of this study was to examine differences in predictors of depression among individuals with FM and OA using a range of health, demographic, and psychological variables. Of the total 963 participants, 600 were diagnosed with FM, and 363 with OA. The Quality of Well-Being Scale (QWB) was used to assess health status. The Fibromyalgia Impact Questionnaire (FIQ) and the Arthritis Impact Measurement Scale (AIMS) were used to measure disease-specific impact. Additionally, participants completed self-efficacy and helplessness assessments. Depression was measured using the Center for Epidemiological Studies Scale (CES-D). The results of a moderated linear regression showed that higher depression scores were associated with lower health status and a greater condition impact, especially in the FM group. Self-efficacy and helplessness predicted depression in both groups, but more strongly in FM. White participants with OA were more depressed than their non-White counterparts, while the opposite was true for FM. These findings indicate that improving health status and psychological well-being might alleviate depression in both FM and OA.
Traumatic Impact Assessment of CPR Load on a Human Ribcage
Authors: Aguilar-Pérez Luis Antonio , Torres-SanMiguel Christopher René , Ceccarelli Marco , Urriolagoitia-Calderón Guillermo Manuel
Published: March 2022

Chest compression is a parameter of injury criteria assessment for human beings. Additionally, it is used to find the external compression response as a result of vehicle crashes, falls, or sporting impacts. This behavioral feature is described by many deterministic models related to specific experimental tests, hindering distinct scenarios. The present study evaluates the energy absorbed as a function of rib compression. The proposed model was obtained from three different computed tomography (CT) studies. The anthropometric values are interpolated to obtain a parametric curve for a human rib’s average size. The computed results are compared against an STL-DICOM® file used to obtain a virtual reconstruction of one rib. A numerical model of the behavior of the thorax displacement expressed injury in the human rib model’s stiffness. The proposed model is used to determine the correlation of the input payload versus the numerical stiffness value. The outcome is confirmed by numerical analyses applied to a virtual human rib reconstruction.
Empowering Children as Agents of Change to Foster Resilience in Community: Implementing “Creative Health” in Primary Schools after the Fukushima Nuclear Disaster
Authors: Goto Aya , Lloyd Williams Alison , Okabe Satoko , Koyama Yohei , Koriyama Chihaya , Murakami Michio , Yui Yumiya , Nollet Kenneth E.
Published: March 2022

The “Creative Heath” project, a participatory school activity to foster community resilience, was implemented in Fukushima, Japan, and children’s experiences of the project were assessed both quantitatively and qualitatively. The project consists of three workshops: BODY, FOOD, and ACT, with activities to facilitate students’ scientific and creative thinking, working in teams, presenting, and expressing their opinions. The first two schools participated with 105 students aged 9–11 years old. Before and after each workshop, students were given questionnaires to rate their satisfaction with their own health (BODY), local foods (FOOD), and the community at large (ACT) on a five-level scale, with space to add free comments. Ratings for BODY and FOOD changed significantly, and the proportion of students who increased their rating of an evaluation indicator after each workshop was 25% for BODY, 28% for FOOD, and 25% for ACT. Text analysis of free comments showed that students in the “increased” group appreciated presenting, measuring, learning connections between nutrition and health, and working collaboratively with peers. Children perceived their health and the foods in their community more positively after participating. Moreover, the Creative Health project could be a way to enhance children’s creativity and autonomy as agents of change in the community.
Literature Cases Summarized Based on Their Polysomnographic Findings in Rett Syndrome
Authors: Zhang Xin-Yan , Spruyt Karen
Published: March 2022

Rett syndrome (RTT) is a severe and rare neurodevelopmental disorder affecting mostly girls. In RTT, an impaired sleep pattern is a supportive criterion for the diagnosis, yet little is known regarding the sleep structure and sleep respiratory events. Aiming to delineate sleep by aggregating RTT case (series) data from published polysomnographic studies, seventy-four RTT cases were collected from eleven studies up until 6 February 2022 (PROSPERO: CRD 42020198099). We compared the polysomnographic data within RTT stratifications and to a typically developing population. MECP2 cases demonstrated shortened total sleep time (TST) with increased stage N3 and decreased REM sleep. In cases with CDKL5 mutations, TST was longer and they spent more time in stage N1 but less in stage N3 than those cases affected by MECP2 mutations and a typically developing population. Sleep-disordered breathing was confirmed by the abnormal apnea/hypopnea index of 11.92 ± 23.67/h TST in these aggregated cases. No association of sleep structure with chronological age was found. In RTT, the sleep macrostructure of MECP2 versus CDKL5 cases showed differences, particularly regarding sleep stage N3. A severe REM sleep propensity reduction was found. Aberrant sleep cycling, possibly characterized by a poor REM ‘on switch’ and preponderance in slow and high-voltage sleep, is proposed.
Association of Body Weight and Physical Fitness during the Elementary School Years
Authors: Drenowatz Clemens , Chen Si-Tong , Cocca Armando , Ferrari Gerson , Ruedl Gerhard , Greier Klaus
Published: March 2022

Physical fitness and body weight are key correlates of health. Nevertheless, an increasing number of children display poor physical fitness and high body weight. The aim of this study was to examine the prospective association of physical fitness with body weight throughout the elementary school years with a special emphasis on children with high body weight or poor physical fitness at baseline. A total of 303 Austrian children (55.1% male) completed the German motor test up to eight times over a 4-year time span (between the ages 6 and 10 years). Physical fitness did not differ across quartiles of body weight at baseline. A more pronounced weight gain, however, was associated with an impaired development of physical fitness and this association was more pronounced in children with higher baseline body weight. In addition, the detrimental effects of an impaired development of physical fitness on subsequent body weight were more pronounced in children with higher baseline body weight. No differences in the longitudinal association between body weight and physical fitness, on the other hand, were observed across quartiles of baseline fitness. These results emphasize the importance of the promotion of physical fitness, particularly in children with increased body weight, to ensure future health.
Analysis of Peri-Implant Bone Loss with a Convergent Transmucosal Morphology: Retrospective Clinical Study
Authors: Costa Castillo María , Laguna Martos Martín , Marco Pitarch Rocío , García Selva Marina , del Cid Rodríguez Silvia , Fons-Badal Carla , Agustín Panadero Rubén
Published: March 2022

Objective: The aim of this study was to analyze the peri-implant bone loss of infracrestal, supracrestal, and crestal implants from the day of placement and up to 1 year of prosthetic loading. Material and methods: A retrospective clinical study was carried out. The sample consisted of 30 implants placed on 30 patients. It was divided into three groups: infracrestal (n = 10), crestal (n = 10), and supracrestal (n = 10) implants. Results: Following the statistical analysis, it was observed that, 4 months after implant placement, the mean values of total peri-implant bone loss were 0.04 mm in infracrestal implants, 0.26 mm in crestal implants, and 0.19 mm in supracrestal implants. At the end of one year of prosthetic loading, the peri-implant bone loss was 0.12 mm in infracrestal implants, 1.04 mm in crestal implants, and 0.27 mm in supracrestal implants. It was determined that peri-implant bone loss in crestal implants was significantly higher than in supracrestal implants, and these in turn were significantly higher than in infracrestal implants. Conclusions: The implants that obtained a better biological behavior on peri-implant bone tissue were the infracrestal implants with a converging transmucosal abutment.
Can Teledentistry Replace Conventional Clinical Follow-Up Care for Minor Dental Surgery? A Prospective Randomized Clinical Trial
Authors: Heimes Diana , Luhrenberg Philipp , Langguth Nils , Kaya Sebahat , Obst Christine , Kämmerer Peer W.
Published: March 2022

(1) Background: Born out of necessity, the implementation of digital processes experienced significant increase during the COVID-19 pandemic. Here, telemedicine offered a bridge to care and now an opportunity to reinvent virtual and hybrid care models, with the goal of improved healthcare access, outcomes, and affordability. The aim of this monocentric prospective, randomized trial was to compare conventional to telephone follow-up after minor dentoalveolar surgery on the basis of special aftercare questionnaires. (2) Methods: Sixty patients who underwent dentoalveolar surgery under local anesthesia were randomly assigned to both groups. After an average of four days, either telephone follow-up (test) or conventional personal aftercare (control) was performed. Based on the questionnaire, the following subject areas were evaluated: symptoms, complications, satisfaction with practitioner, travel, and waiting time, as well as the preferred form of follow-up care. (3) Results: There was no statistically significant difference regarding frequency of symptoms or complication rate. Patients who were assigned to the test group showed a clear tendency to prefer telephone follow-up (83.3%) to conventional aftercare (16.7%, p = 0.047). (4) Conclusions: The data suggest high acceptance of telephone-only follow-up after dentoalveolar surgery. The implementation of telemedicine could be a time- and money-saving alternative for both patients and healthcare professionals and provide healthcare access regardless of time and space.
The Effects of Playground Interventions on Accelerometer-Assessed Physical Activity in Pediatric Populations: A Meta-Analysis
Authors: Pfledderer Christopher D. , Kwon Sunku , Strehli Ildiko , Byun Wonwoo , Burns Ryan D.
Published: March 2022

Playgrounds are designed to be a safe, enjoyable, and effective means to promote physical activity in children and adolescents. The purpose of this study was to conduct a meta-analysis to determine the effectiveness of playground interventions for improving accelerometer-assessed ambulatory moderate-to-vigorous physical activity (MVPA) and to identify common aspects of playground interventions that may be beneficial to promote behavior change. An internet database search was performed. The final analyzed sample of studies was obtained from several criteria, including being a playground-based intervention targeting children or adolescents, having a control or comparison group, having an accelerometer-assessed MVPA outcome target variable, and reporting of the mean difference scores’ variability. A random-effects model meta-analysis was employed to obtain pooled effect sizes. Ten studies (n = 10) were analyzed from the internet search. The weighted pooled effect (Hedges’ g) across all studies was Hedges’ g = 0.13, 95% CI: 0.02–0.24, p = 0.023. There was moderate study heterogeneity (I2 = 55.3%) but no evidence for publication bias (p = 0.230). These results suggest that school-based playground interventions have a small effect on increasing accelerometer-assessed MVPA within the pediatric population. The playground should still be an environmental target during school or community-based interventions aimed at providing opportunities to promote MVPA.
Species Discrimination of Three Odontomachus (Formicidae: Ponerinae) Species in Thailand Using Outline Morphometrics
Authors: Samung Yudthana , Chaiphongpachara Tanawat , Ruangsittichai Jiraporn , Sriwichai Patchara , Phayakkaphon Anon , Jaitrong Weeyawat , Dujardin Jean-Pierre , Sumruayphol Suchada
Published: March 2022

All members of the ant genus Odontomachus Latreille, 1804 are venomous ants. Four species in this genus have been identified from Thailand: Odontomachus latidens Mayr, 1867; O. monticola Emery, 1892; O. rixosus Smith, 1757; and O. simillimus Smith, 1758. The three latter species are available and have been used for an outline morphometric study. They display similar morphology, which makes their distinction very difficult except for highly qualified individuals. A total of 80 worker specimens were studied, exploring the contour shapes of their head and pronotum as possible taxonomic characters. The size of each body part was estimated determining the contour perimeter, the values for which were largely overlapping between O. rixosus and O. simillimus; most O. monticola specimens exhibited a significantly larger size. In contrast to the size, each contour shape of the head or pronotum established O. rixosus as the most distinct species. An exploratory data analysis disclosed the higher taxonomic signal of the head contour relative to the pronotum one. The scores obtained for validated reclassification were much better for the head (99%) than for the pronotum (82%). This study supports outline morphometrics of the head as a promising approach to contribute to the morphological identification of ant species, at least for monomorphic workers.
Geographical Variation of Honey Bee (Apis mellifera L. 1758) Populations in South-Eastern Morocco: A Geometric Morphometric Analysis
Authors: Aglagane Abdessamad , Tofilski Adam , Er-Rguibi Omar , Laghzaoui El-Mustapha , Kimdil Latifa , El Mouden El Hassan , Fuchs Stefan , Oleksa Andrzej , Aamiri Ahmed , Aourir Mohamed
Published: March 2022

In Morocco, there are two well-recognised honey bee (Apis mellifera L.) subspecies: A. m. intermissa in the north and A. m. sahariensis in the south-east. The latter subspecies is found in the arid and semiarid climates of the Sahara Desert. In this study, we used honey bees from four areas of south-eastern Morocco which are, to some degree, isolated by arid zones. We analysed the shape and size of the forewings, using the method of geometric morphometrics. The bees from the four areas of south-eastern Morocco differed significantly in terms of wing shape. Moreover, bees from traditional hives were smaller than those from modern hives. The bees from south-eastern Morocco were clearly different from the reference samples obtained from the Morphometric Bee Data Bank in Oberursel, Germany, representing most of the global variation in honey bees. Surprisingly, the bees were also different from A. m. sahariensis, which should occur in the study area, according to earlier studies. This difference could have been caused by introgression with non-native subspecies imported by beekeepers. The distinct honey bees from south-eastern Morocco deserve to be protected. We provide a method for identifying them, which can help protect them.
Distinct Functional Cortico-Striato-Thalamo-Cerebellar Networks in Genetic Generalized and Focal Epilepsies with Generalized Tonic-Clonic Seizures
Authors: Hsieh Hsinyu , Xu Qiang , Yang Fang , Zhang Qirui , Hao Jingru , Liu Gaoping , Liu Ruoting , Yu Qianqian , Zhang Zixuan , Xing Wei , Bernhardt Boris C. , Lu Guangming , Zhang Zhiqiang
Published: March 2022

This study aimed to delineate cortico-striato-thalamo-cerebellar network profiles based on static and dynamic connectivity analysis in genetic generalized and focal epilepsies with generalized tonic-clonic seizures, and to evaluate its potential for distinguishing these two epilepsy syndromes. A total of 342 individuals participated in the study (114 patients with genetic generalized epilepsy with generalized tonic-clonic seizures (GE-GTCS), and 114 age- and sex-matched patients with focal epilepsy with focal to bilateral tonic-clonic seizure (FE-FBTS), 114 healthy controls). Resting-state fMRI data were examined through static and dynamic functional connectivity (dFC) analyses, constructing cortico-striato-thalamo-cerebellar networks. Network patterns were compared between groups, and were correlated to epilepsy duration. A pattern-learning algorithm was applied to network features for classifying both epilepsy syndromes. FE-FBTS and GE-GTCS both presented with altered functional connectivity in subregions of the motor/premotor and somatosensory networks. Among these two groups, the connectivity within the cerebellum increased in the static, while the dFC variability decreased; conversely, the connectivity of the thalamus decreased in FE-FBTS and increased in GE-GTCS in the static state. Connectivity differences between patient groups were mainly located in the thalamus and cerebellum, and correlated with epilepsy duration. Support vector machine (SVM) classification had accuracies of 66.67%, 68.42%, and 77.19% when using static, dynamic, and combined approaches to categorize GE-GTCS and FE-GTCS. Network features with high discriminative ability predominated in the thalamic and cerebellar connectivities. The network embedding of the thalamus and cerebellum likely plays an important differential role in GE-GTCS and FE-FBTS, and could serve as an imaging biomarker for differential diagnosis.
Ethical Lessons from an Intensivist’s Perspective
Authors: Vincent Jean-Louis
Published: March 2022

Intensive care units (ICUs) around the world have been hugely impacted by the SARS-CoV-2 pandemic and the vast numbers of patients admitted with COVID-19, requiring respiratory support and prolonged stays. This pressure, with resulting shortages of ICU beds, equipment, and staff has raised ethical dilemmas as physicians have had to determine how best to allocate the sparse resources. Here, we reflect on some of the major ethical aspects of the COVID-19 pandemic, including resource allocation and rationing, end-of-life decision-making, and communication and staff support. Importantly, these issues are regularly faced in non-pandemic ICU patient management and useful lessons can be learned from the discussions that have occurred as a result of the COVID-19 situation.
Electrocardiogram Risk Score and Prevalence of Subclinical Atherosclerosis: A Cross-Sectional Study
Authors: Kang Minji , Chang Yoosoo , Kang Jeonggyu , Kim Yejin , Ryu Seungho
Published: March 2022

Integrated abnormal electrocardiogram (ECG) parameters predict the risk of cardiovascular disease (CVD); however, its relationship with subclinical CVD is unknown. We aimed to evaluate the association between the integrated ECG risk score and the prevalence of coronary artery calcium (CAC). A cross-sectional study comprised 134,802 participants with no known CVD who underwent ECG and CAC computed tomography. The ECG risk score was the sum of five ECG abnormalities: heart rate of >80 beats, QRS of >110 ms, left ventricular hypertrophy, T-wave inversion, and prolonged QTc. A multinomial regression model was used to estimate the prevalence ratios (PRs) and their 95% confidence intervals (CIs) for prevalent CAC. The prevalence of CAC progressively increased as the ECG risk score increased. After adjustment for conventional CVD risk factors and other confounders, the multivariable-adjusted PRs (95% CI) for a CAC of 1–100 in the 1, 2, and ≥3 ECG risk score groups were 1.06 (1.02–1.10), 1.12 (1.03–1.22), and 1.19 (1.00–1.42), respectively, while the corresponding PRs for a CAC of >100 were 1.03 (0.95–1.12), 1.44 (1.25–1.66), and 1.75 (1.33–2.29), respectively. Integrative ECG scoring may help identify individuals requiring lipid-lowering medications, even in young and asymptomatic populations.
Secondary Metabolites of Actinomycetales as Potent Quorum Sensing Inhibitors Targeting Gram-Positive Pathogens: In Vitro and In Silico Study
Authors: Desouky Said E. , Abu-Elghait Mohammed , Fayed Eman A. , Selim Samy , Yousuf Basit , Igarashi Yasuhiro , Abdel-Wahab Basel A. , Mohammed Alsuhaibani Amnah , Sonomoto Kenji , Nakayama Jiro
Published: March 2022

Anti-virulence agents are non-bacteriostatic and non-bactericidal emerging therapeutic options which hamper the production of virulence factors in pathogenic flora. In Staphylococcus aureus and Enterococcus faecalis, regulation of virulence genes’ expression occurs through the cyclic peptide-mediated accessory gene regulator (agr) and its ortholog fsr quorum sensing systems, respectively. In the present study, we screened a set of 54 actinomycetales secondary metabolites as novel anti-virulence compounds targeting quorum sensing system of the Gram-positive bacteria. The results indicated that four compounds, Phenalinolactones A–D, BU–4664LMe, 4,5-dehydrogeldamycin, and Questinomycin A, potentially inhibit the agr quorum sensing system and hemolytic activity of S. aureus. On the other hand, Decatromicin A and B, Okilactomycin, Rishirilide A, Abyssomicin I, and Rebeccamycin selectively blocked the fsr quorum sensing system and the gelatinase production in E. faecalis at sub-lethal concentrations. Interestingly, Synerazol uniquely showed the capability to inhibit both fsr and agr quorum sensing systems. Further, in silico molecular docking studies were performed which provided closer insights into the mode of action of these compounds and proposed that the inhibitory activity of these compounds could be attributed to their potential ability to bind to the ATP-active site of S. aureus AgrA. Taken together, our study highlights the potential of actinomycetales secondary metabolites with diverse structures as anti-virulence quorum sensing inhibitors.
Control Strategies of Clubroot Disease Caused by Plasmodiophora brassicae
Authors: Struck Christine , Rüsch Stefanie , Strehlow Becke
Published: March 2022

The clubroot disease caused by the soil-borne pathogen Plasmodiophora brassicae is one of the most important diseases of cruciferous crops worldwide. As with many plant pathogens, the spread is closely related to the cultivation of suitable host plants. In addition, temperature and water availability are crucial determinants for the occurrence and reproduction of clubroot disease. Current global changes are contributing to the widespread incidence of clubroot disease. On the one hand, global trade and high prices are leading to an increase in the cultivation of the host plant rapeseed worldwide. On the other hand, climate change is improving the living conditions of the pathogen P. brassicae in temperate climates and leading to its increased occurrence. Well-known ways to control efficiently this disease include arable farming strategies: growing host plants in wide crop rotations, liming the contaminated soils, and using resistant host plants. Since chemical control of the clubroot disease is not possible or not ecologically compatible, more and more alternative control options are being investigated. In this review, we address the challenges for its control, with a focus on biological control options.
Recent Research in Cell Stress and Microbial Infection
Authors: Trinh Quang Duy
Published: March 2022

Microbial infection, including bacterial, viral, fungal, and parasitic, is a common human disease leading to various cell stresses [...]