bluuebunny/crossref_metadata_2025 huggingface dataset:
Value Co-Creation on TV Talent Shows: Cases from Mainland China, Taiwan and Hong Kong
Authors: Ng Wai-Kit , Yang Cheng-Ming , Chen Chun-Liang
Published: January 2024

Through the actions and interactions of video platform users, talent shows have expanded from the entertainment sphere to the social sphere and become an everyday part of life. Watching talent shows on online platforms, especially through participation in multi-platform interaction, is an ever developing and innovative field in many regions. This study adopts a multiple case analysis approach. We analyze and compare three cases of talent shows, examining aspects of their value co-creation, digital platform, dynamic capability and value network through an exploration of a series of creative activities on digital video platforms. Talent shows provide a unique environment in which different actors interact, co-exist and co-create value, i.e., another form of O2O marketing. These actors include producers, entertainment companies, sponsors and fans, and fan value co-creation currently takes many different forms, which are experienced, engaged and interacted with through different platforms. The findings contribute to examining the underlying dynamics of TV talent shows, in addition to explaining how they are achieving sustainable advantages in the media market. Furthermore, this study aims to understand the service ecosystem of network talent shows from the perspective of industrial innovation strategy; consequently, this research can help to promote the implications of this new form of digital content services and its innovation strategies.
Tolerance of Tall Fescue (Festuca arundinacea Schreb.) Growing in Extensive Green Roof Systems to Saline Water Irrigation with Varying Leaching Fractions
Authors: Ntoulas Nikolaos , Papaioannou Georgios , Bertsouklis Konstantinos , Nektarios Panayiotis A.
Published: January 2024

As urbanization intensifies environmental challenges in contemporary cities, widespread green roof installations emerge as a potential solution. This study explores irrigating tall fescue (Festuca arundinacea Schreb.) turfgrass with saline water in extensive green roof systems, aiming to conserve freshwater resources. The objectives include determining the period of saline water tolerance and identifying the leachate electrical conductivity threshold affecting tall fescue’s green coverage. This greenhouse study comprised 24 lysimeters equipped with extensive green roof layering. Treatments included three NaCl irrigation solutions with an electrical conductivity of 3 dS m−1, 6 dS m−1, and 9 dS m−1, while tap water served as the control. Additionally, irrigation treatments were applied at two different regimes, resulting in an average leaching fraction of 0.3 for the low irrigation regime and 0.5 for the high irrigation regime. Tall fescue’s tolerance to saline water was evaluated through the determination of green turf cover (GTC) as well as the clipping dry weight and the leachate electrical conductivity (ECL) draining from the lysimeters. It was found that tall fescue turfgrass growing in extensive green roof systems can tolerate irrigation with water of electrical conductivity up to 9 dS m−1 for extended periods, approximating three months, without GTC declining below 90%, provided that a minimum leaching of 30% is maintained. Furthermore, irrigating with water at 9 dS m−1 resulted in a 24.5% reduction in cumulative clipping dry weight over the four-month study period. The regression analysis between GTC and ECL highlighted a substantial decline in GTC when ECL surpassed the critical threshold of 12.5 dS m−1.
Analysis of Aging Characteristics of Umbrella Skirts of Composite Insulators Operating under the Influence of a Wind and Sand Environment in South Xinjiang
Authors: Zhang Xiaojun , Kuang Shilong , Wu Suzhou , Zhuang Wenbing , He Chunqing
Published: January 2024

To study the influence of multi-factors, such as long sunshine, sand erosion, and so on, in southern Xinjiang, we selected two kinds of composite insulators for the transmission lines in southern Xinjiang to study the aging characteristics of the umbrella skirt surface. The results of scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS) show that the surface roughness of the umbrella skirt is high and there are a large number of micron inorganic particles. The skirt has the characteristics of low C/O element ratio and high Al element content. The results of thermogravimetric analysis and micro infrared test show that the aging depth of the Myli Line skirt after 19 years of operation is 160~190 µm and that of Yuhe Line 1 after 14 years of operation is greater than 180 µm. The plasma discharge method was used to simulate the corona discharge in the actual operation to accelerate the aging of the surface of the umbrella skirt and the hydrophobic recovery of the umbrella skirt was investigated. The results show that the temperature has a great influence on the surface hydrophobic recovery performance after plasma treatment. These results may provide some theoretical guidance and technical support for the selection, operation, and maintenance of composite insulators in Xinjiang.
EPA and DHA Alleviated Chronic Dextran Sulfate Sodium Exposure-Induced Depressive-like Behaviors in Mice and Potential Mechanisms Involved
Authors: Wang Xi-Yu , He Shu-Sen , Zhou Miao-Miao , Li Xiao-Ran , Wang Cheng-Cheng , Zhao Ying-Cai , Xue Chang-Hu , Che Hong-Xia
Published: January 2024

Patients with ulcerative colitis (UC) have higher rates of depression. However, the mechanism of depression development remains unclear. The improvements of EPA and DHA on dextran sulfate sodium (DSS)-induced UC have been verified. Therefore, the present study mainly focused on the effects of EPA and DHA on UC-induced depression in C57BL/6 mice and the possible mechanisms involved. A forced swimming test and tail suspension experiment showed that EPA and DHA significantly improved DSS-induced depressive-like behavior. Further analysis demonstrated that EPA and DHA could significantly suppress the inflammation response of the gut and brain by regulating the NLRP3/ASC signal pathway. Moreover, intestine and brain barriers were maintained by enhancing ZO-1 and occludin expression. In addition, EPA and DHA also increased the serotonin (5-HT) concentration and synaptic proteins. Interestingly, EPA and DHA treatments increased the proportion of dominant bacteria, alpha diversity, and beta diversity. In conclusion, oral administration of EPA and DHA alleviated UC-induced depressive-like behavior in mice by modulating the inflammation, maintaining the mucosal and brain barriers, suppressing neuronal damage and reverting microbiota changes.
Fluid Flow Dynamics in Partially Saturated Paper
Authors: Kumar Ashutosh , Hatayama Jun , Soucy Alex , Carpio Ethan , Rahmani Nassim , Anagnostopoulos Constantine , Faghri Mohammad
Published: January 2024

This study presents an integrated approach to understanding fluid dynamics in Microfluidic Paper-Based Analytical Devices (µPADs), combining empirical investigations with advanced numerical modeling. Paper-based devices are recognized for their low cost, portability, and simplicity and are increasingly applied in health, environmental monitoring, and food quality analysis. However, challenges such as lack of flow control and the need for advanced detection methods have limited their widespread adoption. To address these challenges, our study introduces a novel numerical model that incorporates factors such as pore size, fiber orientation, and porosity, thus providing a comprehensive understanding of fluid dynamics across various saturation levels of paper. Empirical results focused on observing the wetted length in saturated paper substrates. The numerical model, integrating the Highly Simplified Marker and Cell (HSMAC) method and the High Order accuracy scheme Reducing Numerical Error Terms (HORNET) scheme, successfully predicts fluid flow in scenarios challenging for empirical observation, especially at high saturation levels. The model effectively mimicked the Lucas–Washburn relation for dry paper and demonstrated the increasing time requirement for fluid movement with rising saturation levels. It also accurately predicted faster fluid flow in Whatman Grade 4 filter paper compared with Grade 41 due to its larger pore size and forecasted an increased flow rate in the machine direction fiber orientation of Whatman Grade 4. These findings have significant implications for the design and application of µPADs, emphasizing the need for precise control of fluid flow and the consideration of substrate microstructural properties. The study’s combination of empirical data and advanced numerical modeling marks a considerable advancement in paper-based microfluidics, offering robust frameworks for future development and optimization of paper-based assays.
Effect of Bi2O3–CuO Flux on the Microstructure, Soft Magnetic Properties, and Gyromagnetic Properties of NiCuZn Ferrites for LTCC Devices
Authors: Lu Xiaoling , Zhang Lei
Published: January 2024

In this work, the electromagnetic properties of Ni0.22Cu0.31Zn0.47Fe2O4 (NiCuZn) ferrites doped with 0.3 wt% Bi2O3 + xCuO flux (x = 0.2, 0.4, 0.6, and 0.8 wt%) were studied. Doping resulted in a reduction in the sintering temperature to 900 °C. The doped ferrites were synthesized via the solid-state method. XRD patterns revealed that the prepared ferrites had a cubic spinel structure; thus, a moderate addition of flux did not change the crystal structure. The SEM images, as well as the density and grain size distribution of the samples, showed that the NiCuZn ferrites had densified, homogenized, and contained fully grown grains for x = 0.6 wt%. The sample exhibited good soft magnetic properties, with μ′ reaching the maximum value of 245.4 for x = 0.6 wt% and ε′, Ms, and Hc reaching the maximum values of 23.1, 28.06 emu/g, and 45.86 Oe for x = 0.8 wt%, respectively. Furthermore, the ferrites exhibited good gyromagnetic properties, with 4πMs reaching the maximum value of 1744 Gauss for x = 0.8 wt% and ΔH reaching the minimum value of 228 Oe for x = 0.6 wt%. NiCuZn ferrites were successfully sintered at a lower temperature (900 °C) by adding Bi2O3–CuO flux through LTCC technology and exhibited good soft magnetic properties and gyromagnetic properties. We envisage that these ferrites could be used in multilayer devices.
Instant Candida albicans Detection Using Ultra-Stable Aptamer Conjugated Gold Nanoparticles
Authors: Clack Kimberley , Sallam Mohamed , Muyldermans Serge , Sambasivam Prabhakaran , Nguyen Cong Minh , Nguyen Nam-Trung
Published: January 2024

Fungal pathogens such as Candida albicans have significant impacts on women’s health and the economy worldwide. Current detection methods often require access to laboratory facilities that are costly, inconvenient, and slow to access. This often leads to self-diagnosis, self-treatment and eventual antifungal resistance. We have created a rapid (within five minutes), cost-effective, and user-friendly method for the early detection of Candida albicans. Our platform utilises aptamer-tagged-gold-core-shell nanoparticles for Candida albicans detection based on the presence of 1,3-β-d glucan molecules. Nanoparticle aggregation occurs in the presence of Candida albicans fungal cells, causing a redshift in the UV-visible absorbance, turning from pink/purple to blue. This colour change is perceptible by the naked eye and provides a “yes”/“no” result. Our platform was also capable of detecting Candida albicans from individual yeast colonies without prior sample processing, dilution or purification. Candida albicans yeast cells were detected with our platform at concentrations as low as 5 × 105 cells within a 50 μL sample volume. We believe that this technology has the potential to revolutionise women’s health, enabling women to test for Candida albicans accurately and reliably from home. This approach would be advantageous within remote or developing areas.
Design and Implementation of a Flexible Electromagnetic Actuator for Tunable Terahertz Metamaterials
Authors: Zhou Shengru , Liang Chao , Mei Ziqi , Xie Rongbo , Sun Zhenci , Li Ji , Zhang Wenqiang , Ruan Yong , Zhao Xiaoguang
Published: January 2024

Actuators play a crucial role in microelectromechanical systems (MEMS) and hold substantial potential for applications in various domains, including reconfigurable metamaterials. This research aims to design, fabricate, and characterize structures for the actuation of the EMA. The electromagnetic actuator overcomes the lack of high drive voltage required by other actuators. The proposed actuator configuration comprises supporting cantilever beams with fixed ends, an integrated coil positioned above the cantilever’s movable plate, and a permanent magnet located beneath the cantilever’s movable plate to generate a static magnetic field. Utilizing flexible polyimide, the fabrication process of the EMA is simplified, overcoming limitations associated with silicon-based micromachining techniques. Furthermore, this approach potentially enables large-scale production of EMA, with displacement reaching up to 250 μm under a 100 mA current, thereby expanding their scope of applications in manufacturing. To demonstrate the function of the EMA, we integrated it with a metamaterial structure to form a compact, tunable terahertz absorber, demonstrating a potential for reconfigurable electromagnetic space.
Characterization of Sensitivity of Time Domain MEMS Accelerometer
Authors: Li Enfu , Jian Jiaying , Yang Fan , Ma Zhiyong , Hao Yongcun , Chang Honglong
Published: January 2024

This paper characterizes the sensitivity of a time domain MEMS accelerometer. The sensitivity is defined by the increment in the measured time interval per gravitational acceleration. Two sensitivities exist, and they can be enhanced by decreasing the amplitude and frequency. The sensitivity with minor nonlinearity is chosen to evaluate the time domain sensor. The experimental results of the developed accelerometer demonstrate that the sensitivities span from −68.91 μs/g to −124.96 μs/g and the 1σ noises span from 8.59 mg to 6.2 mg (amplitude of 626 nm: −68.91 μs/g and 10.21 mg; amplitude of 455 nm: −94.51 μs/g and 7.76 mg; amplitude of 342 nm: −124.96 μs/g and 6.23 mg), which indicates the bigger the amplitude, the smaller the sensitivity and the bigger the 1σ noise. The adjustable sensitivity provides a theoretical foundation for range self-adaption, and all the results can be extended to other time domain inertial sensors, e.g., a gyroscope or an inclinometer.
Cohort Profile: The Zurich Primary HIV Infection Study
Authors: Freind Matt C. , Tallón de Lara Carmen , Kouyos Roger D. , Wimmersberger David , Kuster Hebert , Aceto Leonardo , Kovari Helen , Flepp Markus , Schibli Adrian , Hampel Benjamin , Grube Christina , Braun Dominique L. , Günthard Huldrych F.
Published: January 2024

The Zurich Primary HIV Infection (ZPHI) study is a longitudinal cohort study established in 2002, aiming to study the clinical, epidemiological, and biological characteristics of primary HIV infection. The ZPHI enrolls individuals with documented primary HIV-1 infection. At the baseline and thereafter, the socio-demographic, clinical, and laboratory data are systematically collected, and regular blood sampling is performed for biobanking. By the end of December 2022, 486 people were enrolled, of which 353 were still undergoing active follow-up. Of the 486 participants, 86% had an acute infection, and 14% a recent HIV-1 infection. Men who have sex with men accounted for 74% of the study population. The median time from the estimated date of infection to diagnosis was 32 days. The median time from diagnosis to the initiation of antiretroviral therapy was 11 days, and this has consistently decreased over the last two decades. During the seroconversion phase, 447 (92%) patients reported having symptoms, of which only 73% of the patients were classified as having typical acute retroviral syndrome. The ZPHI study is a well-characterized cohort belonging to the most extensively studied primary HIV infection cohort. Its findings contribute to advancing our understanding of the early stages of HIV infection and pathogenesis, and it is paving the way to further improve HIV translational research and HIV medicine.
Harnessing the Biocontrol Potential of Bradyrhizobium japonicum FCBP-SB-406 to Manage Charcoal Rot of Soybean with Increased Yield Response for the Development of Sustainable Agriculture
Authors: Khalid Umar , Aftab Zill-e-Huma , Anjum Tehmina , Bokhari Najat A. , Akram Waheed , Anwar Waheed
Published: January 2024

Plant growth-promoting bacteria (PGPRs) have the potential to act as biofertilizers and biopesticides. This study was planned to explore indigenously isolated PGPRs as a potential candidate to control charcoal rot that affects various crops including soybean. Among the four different tested species of PGPRs, Bradyrhizobium japonicum (FCBP-SB-406) showed significant potential to enhance growth and control soil borne pathogens such as Macrophomina phaseolina. Bacillus subtilis (FCBP-SB-324) followed next. Bradyrhizobium japonicum (FCBP-SB-406) reduced disease severity up to 81.25% in comparison to the control. The strain showed a strong fertilizing effect as a highly significant increase in biomass and other agronomic parameters was recorded in plants grown in its presence. The same was supported by the Pearson’s correlation and principal component analysis. A decrease in disease incidence and severity may be due to the induced resistance imparted by the bacterium. This resulted in significant increments in quantities of defense enzymes, including catalase, peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD). A significant production of proteases, catalases and hydrogen cyanide by B. japonicum (FCBP-SB-406) can also be associated to mycoparasitism. The establishment of PGPRs in treated soils also showed positive effects on soil health. Total metabolite profiling of treated plants in comparison to the control showed the upregulation of many flavonoids, isoflavonoids and amino acids. Many of these compounds have been well reported with antimicrobial activities. Bradyrhizobium japonicum (FCBP-SB-406) can be employed for the production of a potential formulation to support sustainable agriculture by reducing the input of synthetic pesticides and fertilizers.
Polymer Nanoparticles with 2-HP-β-Cyclodextrin for Enhanced Retention of Uptake into HCE-T Cells
Authors: Qin Zhenmiao , Li Baohua , Deng Qiyi , Wen Yifeng , Feng Shiquan , Duan Chengcheng , Zhao Beicheng , Li Hailong , Gao Yanan , Ban Junfeng
Published: January 2024

Triamcinolone acetonide (TA), a medium-potency synthetic glucocorticoid, is primarily employed to treat posterior ocular diseases using vitreous injection. This study aimed to design novel ocular nanoformulation drug delivery systems using PLGA carriers to overcome the ocular drug delivery barrier and facilitate effective delivery into the ocular tissues after topical administration. The surface of the PLGA nanodelivery system was made hydrophilic (2-HP-β-CD) through an emulsified solvent volatilization method, followed by system characterization. The mechanism of cellular uptake across the corneal epithelial cell barrier used rhodamine B (Rh-B) to prepare fluorescent probes for delivery systems. The triamcinolone acetonide (TA)-loaded nanodelivery system was validated by in vitro release behavior, isolated corneal permeability, and in vivo atrial hydrodynamics. The results indicated that the fluorescent probes, viz., the Rh-B-(2-HP-β-CD)/PLGA NPs and the drug-loaded TA-(2-HP-β-CD)/PLGA NPs, were within 200 nm in size. Moreover, the system was homogeneous and stable. The in vitro transport mechanism across the epithelial barrier showed that the uptake of nanoparticles was time-dependent and that NPs were actively transported across the epithelial barrier. The in vitro release behavior of the TA-loaded nanodelivery systems revealed that (2-HP-β-CD)/PLGA nanoparticles could prolong the drug release time to up to three times longer than the suspensions. The isolated corneal permeability demonstrated that TA-(2-HP-β-CD)/PLGA NPs could extend the precorneal retention time and boost corneal permeability. Thus, they increased the cumulative release per unit area 7.99-fold at 8 h compared to the suspension. The pharmacokinetics within the aqueous humor showed that (2-HP-β-CD)/PLGA nanoparticles could elevate the bioavailability of the drug, and its Cmax was 51.91 times higher than that of the triamcinolone acetonide aqueous solution. Therefore, (2-HP-β-CD)/PLGA NPs can potentially elevate transmembrane uptake, promote corneal permeability, and improve the bioavailability of drugs inside the aqueous humor. This study provides a foundation for future research on transocular barrier nanoformulations for non-invasive drug delivery.
The Degradation of Aqueous Oxytetracycline by an O3/CaO2 System in the Presence of HCO3−: Performance, Mechanism, Degradation Pathways, and Toxicity Evaluation
Authors: Li Zedian , Xiang Liangrui , Pan Shijia , Zhu Dahai , Li Shen , Guo He
Published: January 2024

This research constructed a novel O3/CaO2/HCO3− system to degrade antibiotic oxytetracycline (OTC) in water. The results indicated that CaO2 and HCO3− addition could promote OTC degradation in an O3 system. There is an optimal dosage of CaO2 (0.05 g/L) and HCO3− (2.25 mmol/L) that promotes OTC degradation. After 30 min of treatment, approximately 91.5% of the OTC molecules were eliminated in the O3/CaO2/HCO3− system. A higher O3 concentration, alkaline condition, and lower OTC concentration were conducive to OTC decomposition. Active substances including ·OH, 1O2, ·O2−, and ·HCO3− play certain roles in OTC degradation. The production of ·OH followed the order: O3/CaO2/HCO3− > O3/CaO2 > O3. Compared to the sole O3 system, TOC and COD were easier to remove in the O3/CaO2/HCO3− system. Based on DFT and LC-MS, active species dominant in the degradation pathways of OTC were proposed. Then, an evaluation of the toxic changes in intermediates during OTC degradation was carried out. The feasibility of O3/CaO2/HCO3− for the treatment of other substances, such as bisphenol A, tetracycline, and actual wastewater, was investigated. Finally, the energy efficiency of the O3/CaO2/HCO3− system was calculated and compared with other mainstream processes of OTC degradation. The O3/CaO2/HCO3− system may be considered as an efficient and economical approach for antibiotic destruction.
Chemical Epigenetic Regulation Secondary Metabolites Derived from Aspergillus sydowii DL1045 with Inhibitory Activities for Protein Tyrosine Phosphatases
Authors: Shi Xuan , Li Xia , He Xiaoshi , Zhang Danyang , Quan Chunshan , Xiu Zhilong , Dong Yuesheng
Published: January 2024

Protein tyrosine phosphatases (PTPs) are ubiquitous in living organisms and are promising drug targets for cancer, diabetes/obesity, and autoimmune disorders. In this study, a histone deacetylase inhibitor called suberoylanilide hydroxamic acid (SAHA) was added to a culture of marine fungi (Aspergillus sydowii DL1045) to identify potential drug candidates related to PTP inhibition. Then, the profile of the induced metabolites was characterized using an integrated metabolomics strategy. In total, 46% of the total SMs were regulated secondary metabolites (SMs), among which 20 newly biosynthesized metabolites (10% of the total SMs) were identified only in chemical epigenetic regulation (CER) broth. One was identified as a novel compound, and fourteen compounds were identified from Aspergillus sydowii first. SAHA derivatives were also biotransformed by A. sydowii DL1045, and five of these derivatives were identified. Based on the bioassay, some of the newly synthesized metabolites exhibited inhibitory effects on PTPs. The novel compound sydowimide A (A11) inhibited Src homology region 2 domain-containing phosphatase-1 (SHP1), T-cell protein tyrosine phosphatase (TCPTP) and leukocyte common antigen (CD45), with IC50 values of 1.5, 2.4 and 18.83 μM, respectively. Diorcinol (A3) displayed the strongest inhibitory effect on SHP1, with an IC50 value of 0.96 μM. The structure–activity relationship analysis and docking studies of A3 analogs indicated that the substitution of the carboxyl group reduced the activity of A3. Research has demonstrated that CER positively impacts changes in the secondary metabolic patterns of A. sydowii DL1045. The compounds produced through this approach will provide valuable insights for the creation and advancement of novel drug candidates related to PTP inhibition.
Biochemical Profiling and Physicochemical and Biological Valorization of Iraqi Honey: A Comprehensive Analysis
Authors: Hameed Omar Mohammed , Shaker Ohood Mzahim , Ben Slima Ahlem , Makni Mohamed
Published: January 2024

Our study aimed to analyze five monovarietal honeys from the Salah Eddine region in Iraq, focusing on physicochemical, antioxidant, and antimicrobial properties and polyphenolic compounds. Our objective was to evaluate the strengths and qualities of Iraqi honeys, ensuring compliance with the Codex Alimentarius standard for honey. The spectrophotometric analysis included assessments of reduced sugar (75.8–77.7%), fructose-to-glucose ratio (0.7–0.9%), sucrose (2.2–2.9%), HMF (17.23–18.87 mg/kg), and melanoidin content (0.25–0.44), which were all determined. The electrical conductivity (0.39–0.46 mS/cm) using a conductivity meter, pH (4.02–4.31), and mineral composition were determined in all samples using atomic absorption spectrometry. Antioxidant activities were spectrophotometrically determined, through DPPH free radical scavenging (7.87–95.62 mg/mL), as was the total antioxidant activity (14.26–22.15 mg AAE/g), with correlations established with biochemical constituents such as the total phenol content, highlighting the significant presence of Coumaric acid (0.38–2.34 µg/mL), Catechin (1.80–2.68 µg/mL), and Quercetin (0.30 µg/mL) using HPLC. The study also observed notable antimicrobial activities using Escherichia coli, Staphylococcus aureus, and Candida albicans on Mueller–Hinton agar as well as through diffusion technique. In conclusion, our findings, including the antioxidant and antimicrobial strengths, underscore the substantial potential of Iraqi honeys in mitigating damage and preventing the onset of various diseases, affirming their good quality and adherence to international honey standards.
Assessment of Selected Surface and Electrochemical Properties of Boron and Strontium-Substituted Hydroxyapatites
Authors: Kolmas Joanna , Samoilov Pavlo , Jaguszewska Aneta , Skwarek Ewa
Published: January 2024

Tissue engineering is an interdisciplinary field of science that has been developing very intensively over the last dozen or so years. New ways of treating damaged tissues and organs are constantly being sought. A variety of porous structures are currently being investigated to support cell adhesion, differentiation, and proliferation. The selection of an appropriate biomaterial on which a patient’s new tissue will develop is one of the key issues when designing a modern tissue scaffold and the associated treatment process. Among the numerous groups of biomaterials used to produce three-dimensional structures, hydroxyapatite (HA) deserves special attention. The aim of this paper was to discuss changes in the double electrical layer in hydroxyapatite with an incorporated boron and strontium/electrolyte solution interface. The adsorbents were prepared via dry and wet precipitation and low-temperature nitrogen adsorption and desorption methods. The specific surface area was characterized, and the surface charge density and zeta potential were discussed.
Assessment of Epicardial Fat in Children: Its Role as a Cardiovascular Risk Factor and How It Is Influenced by Lifestyle Habits
Authors: Calcaterra Valeria , Cena Hellas , Garella Vittoria , Loperfido Federica , Chillemi Claudia , Manuelli Matteo , Mannarino Savina , Zuccotti Gianvincenzo
Published: January 2024

Epicardial adipose tissue (EAT) stands out as a distinctive repository of visceral fat, positioned in close anatomical and functional proximity to the heart. EAT has emerged as a distinctive reservoir of visceral fat, intricately interlinked with cardiovascular health, particularly within the domain of cardiovascular diseases (CVDs). The aim of our overview is to highlight the role of EAT as a marker for cardiovascular risk in children. We also explore the influence of unhealthy lifestyle habits as predisposing factors for the deposition of EAT. The literature data accentuate the consequential impact of lifestyle choices on EAT dynamics, with sedentary behavior and unwholesome dietary practices being contributory to a heightened cardiovascular risk. Lifestyle interventions with a multidisciplinary approach are therefore pivotal, involving a nutritionally balanced diet rich in polyunsaturated and monounsaturated fatty acids, regular engagement in aerobic exercise, and psychosocial support to effectively mitigate cardiovascular risks in children. Specific interventions, such as high-intensity intermittent training and circuit training, reveal favorable outcomes in diminishing the EAT volume and enhancing cardiometabolic health. Future clinical studies focusing on EAT in children are crucial for advancing our understanding and developing targeted strategies for cardiovascular risk management in this population.
Pathophysiological-Based Nutritional Interventions in Cirrhotic Patients with Sarcopenic Obesity: A State-of-the-Art Narrative Review
Authors: Santangeli Ernestina , Abbati Chiara , Chen Rusi , Di Carlo Alma , Leoni Simona , Piscaglia Fabio , Ferri Silvia
Published: January 2024

In recent decades, following the spread of obesity, metabolic dysfunction has come to represent the leading cause of liver disease. The classical clinical presentation of the cirrhotic patient has, therefore, greatly changed, with a dramatic increase in subjects who appear overweight or obese. Due to an obesogenic lifestyle (lack of physical activity and overall malnutrition, with an excess of caloric intake together with a deficit of proteins and micronutrients), these patients frequently develop a complex clinical condition defined as sarcopenic obesity (SO). The interplay between cirrhosis and SO lies in the sharing of multiple pathogenetic mechanisms, including malnutrition/malabsorption, chronic inflammation, hyperammonemia and insulin resistance. The presence of SO worsens the outcome of cirrhotic patients, affecting overall morbidity and mortality. International nutrition and liver diseases societies strongly agree on recommending the use of food as an integral part of the healing process in the comprehensive management of these patients, including a reduction in caloric intake, protein and micronutrient supplementation and sodium restriction. Based on the pathophysiological paths shared by cirrhosis and SO, this narrative review aims to highlight the nutritional interventions currently advocated by international guidelines, as well as to provide hints on the possible role of micronutrients and nutraceuticals in the treatment of this multifaceted clinical condition.
Hybrid Molecules of Azithromycin with Chloramphenicol and Metronidazole: Synthesis and Study of Antibacterial Properties
Authors: Volynkina Inna A. , Bychkova Elena N. , Karakchieva Anastasiia O. , Tikhomirov Alexander S. , Zatonsky George V. , Solovieva Svetlana E. , Martynov Maksim M. , Grammatikova Natalia E. , Tereshchenkov Andrey G. , Paleskava Alena , Konevega Andrey L. , Sergiev Petr V. , Dontsova Olga A. , Osterman Ilya A. , Shchekotikhin Andrey E. , Tevyashova Anna N.
Published: January 2024

The sustained rise of antimicrobial resistance (AMR) causes a strong need to develop new antibacterial agents. One of the methods for addressing the problem of antibiotic resistance is through the design of hybrid antibiotics. In this work, we proposed a synthetic route for the conjugation of an azithromycin derivative with chloramphenicol and metronidazole hemisuccinates and synthesized two series of new hybrid molecules 4a–g and 5a–g. While a conjugation did not result in tangible synergy for wild-type bacterial strains, new compounds were able to overcome AMR associated with the inducible expression of the ermC gene on a model E. coli strain resistant to macrolide antibiotics. The newly developed hybrids demonstrated a tendency to induce premature ribosome stalling, which might be crucial since they will not induce a macrolide-resistant phenotype in a number of pathogenic bacterial strains. In summary, the designed structures are considered as a promising direction for the further development of hybrid molecules that can effectively circumvent AMR mechanisms to macrolide antibiotics.
Sulfadiazine Exerts Potential Anticancer Effect in HepG2 and MCF7 Cells by Inhibiting TNFα, IL1b, COX-1, COX-2, 5-LOX Gene Expression: Evidence from In Vitro and Computational Studies
Authors: Gomaa Mohamed , Gad Wael , Hussein Dania , Pottoo Faheem Hyder , Tawfeeq Nada , Alturki Mansour , Alfahad Dhay , Alanazi Razan , Salama Ismail , Aziz Mostafa , Zahra Aboelnasr , Hanafy Abeer
Published: January 2024

Drug repurposing is a promising approach that has the potential to revolutionize the drug discovery and development process. By leveraging existing drugs, we can bring new treatments to patients more quickly and affordably. Anti-inflammatory drugs have been shown to target multiple pathways involved in cancer development and progression. This suggests that they may be more effective in treating cancer than drugs that target a single pathway. Cell viability was measured using the MTT assay. The expression of genes related to inflammation (TNFa, IL1b, COX-1, COX-2, and 5-LOX) was measured in HepG2, MCF7, and THLE-2 cells using qPCR. The levels of TNFα, IL1b, COX-1, COX-2, and 5-LOX were also measured in these cells using an ELISA kit. An enzyme binding assay revealed that sulfadiazine expressed weaker inhibitory activity against COX-2 (IC50 = 5.27 μM) in comparison with the COX-2 selective reference inhibitor celecoxib (COX-2 IC50 = 1.94 μM). However, a more balanced inhibitory effect was revealed for sulfadiazine against the COX/LOX pathway with greater affinity towards 5-LOX (IC50 = 19.1 μM) versus COX-1 (IC50 = 18.4 μM) as compared to celecoxib (5-LOX IC50 = 16.7 μM, and COX-1 IC50 = 5.9 μM). MTT assays revealed the IC50 values of 245.69 ± 4.1 µM and 215.68 ± 3.8 µM on HepG2 and MCF7 cell lines, respectively, compared to the standard drug cisplatin (66.92 ± 1.8 µM and 46.83 ± 1.3 µM, respectively). The anti-inflammatory effect of sulfadiazine was also depicted through its effect on the levels of inflammatory markers and inflammation-related genes (TNFα, IL1b, COX-1, COX-2, 5-LOX). Molecular simulation studies revealed key binding interactions that explain the difference in the activity profiles of sulfadiazine compared to celecoxib. The results suggest that sulfadiazine exhibited balanced inhibitory activity against the 5-LOX/COX-1 enzymes compared to the selective COX-2 inhibitor, celecoxib. These findings highlight the potential of sulfadiazine as a potential anticancer agent through balanced inhibitory activity against the COX/LOX pathway and reduction in the expression of inflammatory genes.
In Vitro Characterization of Reversine-Treated Gingival Fibroblasts and Their Safety Evaluation after In Vivo Transplantation
Authors: Marto Carlos Miguel , Laranjo Mafalda , Gonçalves Ana Cristina , Paula Anabela , Jorge Joana , Caetano-Oliveira Rui , Sousa Maria Inês , Oliveiros Bárbara , Ramalho-Santos João , Sarmento-Ribeiro Ana Bela , Marques-Ferreira Manuel , Cabrita António , Botelho Maria Filomena , Carrilho Eunice
Published: January 2024

Reversine is a purine derivative that has been investigated with regard to its biological effects, such as its anticancer properties and, mostly, its ability to induce the dedifferentiation of adult cells, increasing their plasticity. The obtained dedifferentiated cells have a high potential for use in regenerative procedures, such as regenerative dentistry (RD). Instead of replacing the lost or damaged oral tissues with synthetic materials, RD uses stem cells combined with matrices and an appropriate microenvironment to achieve tissue regeneration. However, the currently available stem cell sources present limitations, thus restricting the potential of RD. Based on this problem, new sources of stem cells are fundamental. This work aims to characterize mouse gingival fibroblasts (GFs) after dedifferentiation with reversine. Different administration protocols were tested, and the cells obtained were evaluated regarding their cell metabolism, protein and DNA contents, cell cycle changes, morphology, cell death, genotoxicity, and acquisition of stem cell characteristics. Additionally, their teratoma potential was evaluated after in vivo transplantation. Reversine caused toxicity at higher concentrations, with decreased cell metabolic activity and protein content. The cells obtained displayed polyploidy, a cycle arrest in the G2/M phase, and showed an enlarged size. Additionally, apoptosis and genotoxicity were found at higher reversine concentrations. A subpopulation of the GFs possessed stem properties, as supported by the increased expression of CD90, CD105, and TERT, the existence of a CD106+ population, and their trilineage differentiation capacity. The dedifferentiated cells did not induce teratoma formation. The extensive characterization performed shows that significant functional, morphological, and genetic changes occur during the dedifferentiation process. The dedifferentiated cells have some stem-like characteristics, which are of interest for RD.
A Surprising Repurposing of Central Nervous System Drugs against Squamous Cell Carcinoma of the Bladder, UM-UC-5
Authors: Gouveia Maria João , Ribeiro Eduarda , Vale Nuno
Published: January 2024

The potential benefits of drug repurposing have gained attention as an alternative to developing de novo drugs. The potential of using central nervous system (CNS) drugs as anticancer drugs has been explored in several types of human cancers, such as breast and colon cancer, among others. Here, we examine the effect of the CNS drugs sertraline, paroxetine, and chlorpromazine on human squamous carcinoma cells of the bladder (UM-UC-5). After exposing UM-UC-5 cells to increased concentrations of each drug for 48 h, we assessed their metabolic activity using an MTT assay. Based on those results, we calculated cell viability and the half-maximal inhibitory concentration (IC50) values. The results suggest that the CNS drugs were effective against UM-UC-5 in the order of potency of sertraline > chlorpromazine > paroxetine. Interestingly, sertraline was more potent than 5-fluorouracil (5-FU), a widely used anticancer drug. This study demonstrated, for the first time, the promising anticancer activity of CNS drugs on human bladder cancer cells in vitro and supports the repurposing of CNS drugs to improve cancer treatment. Nevertheless, further studies are necessary to understand their mechanism of action and in vivo activity.
Design and Optimization of a High-Efficiency 3D Multi-Tip Edge Coupler Based Lithium Niobate on Insulator Platform
Authors: Zhang Tian , Li Jinye , Li Mingxuan , Liu Jianguo
Published: January 2024

Fiber-chip edge couplers can minimize mode mismatch in integrated lithium niobate (LiNbO3) photonics via facilitating broad optical bandwidth coupling between optical fibers and waveguide circuits. We designed a high-efficiency multi-tip edge coupler utilizing the lithium niobate on insulator (LNOI) platform for achieving superior fiber-to-chip coupling. The device comprises a bilayer LN inversely tapered waveguide, three 3D inversely tapered waveguides, and a silicon oxynitride (SiON) cladding waveguide (CLDWG). Finite difference method (FDM) and eigenmode expansion (EME) simulations were utilized to simulate and optimize the edge coupler structure specifically within the 1550 nm band. This coupler demonstrates a low fiber-chip coupling loss of 0.0682/0.0958 dB/facet for TE/TM mode at 1550 nm when interfaced with a commercially cleaved single-mode fiber (SMF) with a mode field diameter (MFD) of approximately 8.2 μm. Moreover, the 1 dB bandwidth of the coupler is 270 nm for the TE mode and 288 nm for the TM mode. Notably, the coupler exhibits a relatively large tolerance for optical misalignment owing to its large mode spot size of up to 4 μm. Given its ultra-low loss, high-efficiency ultra-broadband capabilities, and substantial tolerance features, this proposed device provides a paradigm for fiber-to-chip edge coupling within lithium niobate photonics.
Rapid Access to Empirical Impact Ionization Cross Sections for Atoms and Ions across the Periodic Table
Authors: Fritzsche Stephan , Jiao Liguang , Visentin Giorgio
Published: January 2024

Electron-impact ionization (EII) processes are essential for modelling high-temperature plasma in quite different research areas, from astrophysics to material science to plasma and fusion research and in several places elsewhere. In most, if not all, of these fields, partial and total EII cross sections are required, and often for a good range of electron energies, in order to determine, for instance, the level population of ions and spectral line intensities in plasma under both local and non-local thermodynamic equilibrium conditions. To obey these needs, various kinds of semi-empirical EII cross sections have been applied in practice, often simply because of the large computational demands in dealing explicitly with two free electrons within the continuum. Here, we expand Jac, the Jena Atomic Calculator, to provide such empirical EII cross sections for (most) atoms and ions across the periodic table. Five empirical models from the recent literature have been implemented to support a simple and rapid access to the partial EII cross sections for electrons from a (partly filled) shell (nℓ)q as well as the total ionization cross sections. We here restrict ourselves to the direct part of the EII cross section, whereas the impact excitation of electrons with subsequent autoionization and the resonant electron capture with double autoionization have been left aside in this first implementation. Rapid access to the (direct) EII cross sections will help already to better understand the role of electron-impact processes in the diagnostics of fusion plasma or the interpretation of astrophysical spectra.
The Kinetic Study of the Influence of Common Modifiers on the Curing Process of Epoxy Vitrimers
Authors: Korotkov Roman , Shutov Vyacheslav , Orlov Alexey , Bornosuz Natalia , Kulemza Daria , Onuchin Denis , Shcherbina Anna , Gorbunova Irina , Sirotin Igor
Published: January 2024

An analysis of the influence of common modifiers on the kinetics of the curing process of epoxy-anhydride vitrimers was carried out. As common modifiers to enhance the “vitrimeric” nature of the material, zinc acetylacetonate as a transesterification catalyst and glycerol as a modifier of hydroxyl group content were chosen. The curing process of all obtained compositions was studied by differential scanning calorimetry (DSC) followed by the application of the isoconversional approach. It was shown that additives significantly affect the curing process. The resulting cured polymers were shown to be chemically recyclable by dissolution in the mixture of ethylene glycol and N-methylpirrolidone in a volume ratio of nine to one. The introduction of both zinc acethylacetonate and glycerol to the neat formulation led to a decrease in the dissolution time by 85.7% (from 35 h for the neat epoxy-anhydride formulation to 5 h for the modified formulation). In order to show the opportunity of the secondary use of recyclates, the mixtures based on the basic composition containing 10 wt. % of secondary polymers were also studied. The introduction of a recycled material to neat composition led to the same curing behavior as glycerol-containing systems.
Design of Novel Functional Conductive Structures and Preparation of High-Hole-Mobility Polymer Transistors by Green Synthesis Using Acceptor–Donor–Acceptor Strategies
Authors: Ren Shiwei , Wang Sichun , Chen Jinyang , Yi Zhengran
Published: January 2024

The design of novel acceptor molecular structures based on classical building blocks is regarded as one of the efficient ways to explore the application of organic conjugated materials in conductivity and electronics. Here, a novel acceptor moiety, thiophene-vinyl-diketopyrrolopyrrole (TVDPP), was envisioned and prepared with a longer conjugation length and a more rigid structure than thiophene-diketopyrrolopyrrole (TDPP). The brominated TVDPP can be sequentially bonded to trimethyltin-containing benzo[c][1,2,5]thiadiazole units via Suzuki polycondensation to efficiently prepare the polymer PTVDPP-BSz, which features high molecular weight and excellent thermal stability. The polymerization process takes only 24 h and eliminates the need for chlorinated organic solvents or toxic tin-based reagents. Density functional theory (DFT) simulations and film morphology analyses verify the planarity and high crystallinity of the material, respectively, which facilitates the achievement of high carrier mobility. Conductivity measurements of the polymeric material in the organic transistor device show a hole mobility of 0.34 cm2 V−1 s−1, which illustrates its potential for functionalized semiconductor applications.
Comparison of Mechanical Properties of Steam-Free and Steam-Based Specimens Made of Expanded Polypropylene Beads
Authors: Handtke Sören , Hain Jörg , Fischer Fabian , Ossowski Tim , Dröder Klaus
Published: January 2024

Reducing the CO2 emissions of plastic parts is crucial in terms of sustainable product and process designs. Approaches include the use of recycled materials and reducing the energy demands of processes through more efficient technologies. In this context, this study shows the potential of the steam-free processing of particle foam beads into thin-walled moulded parts. Expanded polypropylene (EPP) particle foam beads have been processed in both a steam-free and steam-based process. For this purpose, specimens with different part densities and thicknesses were produced, the mechanical properties were investigated, and the surface quality was discussed. Specimens made of EPP with a part thickness of 5 to 20 mm and part densities of 60 to 185 g/L were produced steam-free. Lower part thicknesses and higher densities increase the mechanical properties. As the density increased, the homogeneity of the surfaces of the steam-free specimens also increased. In comparison, specimens with a thickness of 10 mm and part densities of 35 to 90 g/L were produced on a steam-based process. The results of the mechanical test were compared with those of the steam-free specimens. The steam-based specimens showed higher mechanical properties for the same density.
Special Issue on “Applications of Chromatographic Separation Techniques in Food and Chemistry”
Authors: Pyka-Pająk Alina
Published: January 2024

Chromatographic techniques and methods are experiencing significant growth in various industries [...]