bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of Cyanotoxin Episodes in Freshwater: A Case Study on Microcystin and Saxitoxin in the Lobo Reservoir, São Paulo State, Brazil
Authors: Moraes Munique de Almeida Bispo , Rodrigues Raphaella de Abreu Magalhães , Podduturi Raju , Jørgensen Niels Ole Gerslev , Calijuri Maria do Carmo
Published: August 2023

Freshwater reservoirs constitute an important source of drinking water, but eutrophication and higher temperatures increase the risk of more frequent blooms of cyanobacteria, including species that produce toxins. To improve the prediction of cyanotoxin episodes, we studied the annual occurrence of potential microcystin (MC) and saxitoxin (STX)-producing cyanobacteria in the Lobo reservoir, São Paulo State, Brazil. Relationships among environmental variables, cyanobacterial biomass, numbers of the mcyE and sxtA genotypes (genes encoding production of MC and STX, respectively), and concentrations of MC and STX were determined to address variables applicable for monitoring and predicting the dynamics of cyanobacteria and cyanotoxins in the reservoir. Microscopy confirmed the presence of potentially toxin-producing cyanobacteria at all sampling times, and qPCR detection showed the occurrence of both mcyE and sxtA in most samples. Concentrations of MC and STX were generally low (MC 0–1.54 µg L−1; STX 0.03–0.21 µg L−1). The highest MC level exceeded the recommended limit for human intake of 1 µg L−1. The abundance of the mcyE and sxtA genes, as well as the toxin concentrations, were positively correlated with the biomass of Phormidium and Raphidiopsis. Among environmental variables, the abundance of potential toxic cyanobacteria was mainly affected by P limitation (high TN:TP ratios). Our data show that detection of the mcyE and sxtA genotypes serves as a useful and reliable predictor of toxin episodes but might be combined with chemical toxin detection to form an environmental toolbox for cyanotoxin monitoring.
Retrospective Assessment of Greenhouse Gas Emissions from the Beef Sector in Greece and Potential Mitigation Scenarios
Authors: Azoukis Stephanos , Akamati Konstantina , Bizelis Iosif , Laliotis George P.
Published: August 2023

Although beef production is one of the most valuable drivers of the global livestock economy, it is considered the main contributor to GHG emissions derived from livestock. This study’s objectives were to estimate the GHG emissions (expressed in carbon dioxide equivalents; CO2-eq) from the beef sector in Greece at the national and prefecture levels during the period 2011–2021 and to explore potential mitigation scenarios. The Tier 1 and 2 methodologies were implemented to estimate the GHG emissions. The total estimated emissions increased over the study period. Although both methodologies captured similar trends in the changes in GHG emissions, the Tier 2 estimations revealed lower emissions and fluctuations due to the different and more precise computational approaches. At the prefecture level, fluctuations in emissions were also noted. However, specific regions showed higher increases in emissions. The observed increase in emissions, in terms of either absolute values (Gg CO2-eq) or intensities (Kg CO2-eq/Kg produced beef carcass), is of utmost importance, and further mitigation strategies should be considered. The regression analysis showed a good predictive ability for emissions, using the number of livestock animals as the input. The equations derived from this analysis could be further used as first-approach tools for capturing future emissions at the national level before proceeding with more elaborate approaches. The different scenarios examined in response to the sector’s challenges showed moderate changes in GHG emissions. Depending on national priorities, such scenarios could serve as pilot case studies, which may assist stakeholders in improving the sustainability of the sector in the future.
A New Method to Calibrate Cardinal Temperatures for Eucalyptus Plantation
Authors: Queiroz Túlio Barroso , Montes Cristian Rodrigo , Campoe Otávio Camargo
Published: August 2023

Developing a good understanding of the interactions between forest plantation growth and climate is essential for predicting the impact of climate change on terrestrial ecosystems and for assessing the adaptation and vulnerability of tree species. One such interaction, the response in growth rate of a forest stand to changes in temperature, may be described mathematically. Some models that run on monthly time steps assume a yearly optimum, minimum, and maximum temperature for simplicity, which may not represent well to actual forest growth. Here, we developed a finer-resolution methodology that encompasses monthly growth rates and temperature limits to calibrate the parameters for an envelope curve in Eucalyptus plantations in South America. Several polynomial curves were tested to determine temperature patterns, and their yearly tree growth patterns demonstrated that responses to temperature differed by as much as 10 °C among seasons. The best curve was a second-degree polynomial curve, whose extreme values indicated the optimum temperature and whose real roots limited the minimum and maximum temperatures for growth. This polynomial was fitted every month to describe yearly changes in optimum, maximum, and minimum temperatures. When fitted to annual data, it determined 7 °C, 19 °C, and 31 °C as the minimum, optimum, and maximum temperatures for tree growth, respectively. The monthly model predictions indicated that the minimum, optimum, and maximum temperatures lay between 8 °C and 16 °C, 18 °C and 22 °C, and 27 °C and 30 °C, respectively. These monthly temperature ranges can improve the estimation of productivity in process-based models. Our results contribute to the understanding of tree growth dynamics and its relationship to changes in temperature. Accurate ranges of temperature can be used to improve productivity predictions in new expanding planting regions with no previous information or to suggest a regionalization for potential species.
Antifreeze Evaluation of Two Dehydrin Proteins from Pseudotsuga menziesii and Larix principis-rupprechtii Mayr
Authors: Liu Xiao , Zhao Qihong , Li Mengtian , Zhang Junkang , Wang Lei , Xu Jichen
Published: August 2023

Dehydrins exist widely in plants and play an important role in abiotic stress resistance. Two low-temperature-induced dehydrin-like genes, PmCAP and LpCAP, from the pine species Pseudotsuga menziesii and Larix principis-rupprechtii Mayr were cloned and found to contain 576 bp and 687 bp, encoding 191 and 228 amino acids, respectively. Both genes were individually assembled into prokaryotic expression vectors and transferred into E. coli cells. When transgenic stains were cultured at −5 °C, the lethal time 50% (LT50) was 50 h and 54 h for PmCAP and LpCAP, respectively, compared with 32 h for the control. When cultured at −20 °C, the LT50 was 38 h, 41 h, and 25 h for PmCAP, LpCAP, and the control. Thermal hysteresis (TH) testing of PmCAP and LpCAP proteins revealed TH values of 0.27 °C and 0.72 °C, respectively, relative to 0.05 °C for the BSA control. These results indicate that the two pine dehydrin proteins have antifreeze characteristics and that their antifreeze levels were well in relation to the environmental conditions of pine growth (Larix principis-rupprechtii Mayr mostly grows in cold and high-altitude zones, while Pseudotsuga menziesii grows in temperate and low-altitude zones). LpCAP, especially, could be a better gene resource for the molecular breeding of plant cold resistance.
Genome-Wide Identification and Analysis of the DGAT Gene Family in Lindera glauca and Expression Analysis during Fruit Development Stages
Authors: Bai Xue , Yang Yongyi , Xie Lun , Li Qingqing , Xiong Biao
Published: August 2023

Diacylglycerol acyltransferase (DGAT) is a vital and sole rate-limiting enzyme involved in triacylglycerol synthesis. Identifying DGAT genes in Lindera glauca is essential for studying lipid metabolism pathways and developing novel oil crops with enhanced value. In the study reported in this paper, 15 LgDGAT family genes were first obtained from the L. glauca genome via bioinformatics analysis. We comprehensively analyzed their chromosome distribution, gene structure, subcellular localization, promoter prediction, phylogenetic relationships, tissue-specific expression, and expression patterns during different stages of fruit development. Our findings revealed that LgDGATs can be classified into DGAT1, DGAT2, DGAT3, and WSD (wax ester synthase/acyl-CoA: diacylglycerol acyltransferase) subfamilies distributed across chromosome 3, 5, 6, 8 and 11. LgDGATs’ promoter region showed abundant elements linked to the light response and plant hormone response. Forms of LgDGAT1, LgDGAT2, and LgDGAT3 were primarily expressed in the early and late phases of fruit development, indicating their potential function in the growth and development of L. glauca, particularly in oil accumulation. Conversely, LgWSDs exhibited predominant expression in stems and leaves. This paper elucidates the gene structure and expression patterns of LgDGATs, providing a theoretical foundation for understanding the functionality of DGAT genes in Lindera species.
Plant–Soil Feedback of Companion Species during Grassland Community Succession
Authors: Zhang Li , Zhang Linhui , Huang Lulu , Zhou Huiling , Xue Sha , Wang Minggang , Xu Hongwei
Published: August 2023

The responses of dominant species to plant–soil feedback (PSF) are well established; however, the changes in the PSF of companion species remain unclear. This study aims to assess the direction and intensity of PSF, determine the main factors influencing it, and interpret the ecological significance of PSF in companion species within the context of grassland community succession. Three typical companion species, namely Artemisia sacrorum, Artemisia capillaris, and Artemisia giraldii, were planted in soils at three grassland community succession stages (early, middle, and late) on the Loess Plateau. Our results indicate that during both plant growth periods, the shoot biomass of A. sacrorum, A. capillaris, and A. giraldii in early- and late-stage soils was higher than that in the middle-stage soil, suggesting consistent growth of the three companion species during the two growth periods. However, plant growth simultaneously led to a reduction in soil nutrient content and microbial biomass, resulting in an overall decrease in the biomass of the three species, indicating a negative PSF effect in companion species. In conclusion, the negative PSF observed in all three associated species explains the temporary dominance of companion species during succession. This study enhances our understanding of the mechanisms driving PSF in community succession.
Properties of Plywood Made of Thermally Treated Veneers Bonded with Castor Oil-Based Polyurethane Adhesive
Authors: Galdino Danilo Soares , Silva Maria Fernanda Felippe , Arroyo Felipe Nascimento , Rangel Elidiane Cipriano , Caraschi José Cláudio , Santos Herisson Ferreira dos , de Freitas Ludmila , Christoforo André Luis , de Campos Cristiane Inácio
Published: August 2023

Wood industries use thermal and thermomechanical treatments as ecological approaches to increase the durability of wood products, avoiding the need for chemical additives. In this regard, the aim of this study was to compare the physical and mechanical properties of plywood made from veneers treated at different temperatures using thermal and thermomechanical processes, with untreated panels serving as a control. The treatment process involved Pinus taeda veneers submitted to treatment in a hot press at 1.0 MPa in a laboratory oven at temperatures of 160 °C, 180 °C, and 200 °C for 30 min. For bonding the veneers, a vegetable-based polyurethane resin derived from castor oil with a grammage of 395 g/m2 was used, applying pressing conditions at 90 °C, 0.6 MPa, and 10 min. Our results indicate that temperature significantly influences plywood properties, playing a key role in the choice of equipment for the treatment process. Regardless of the method employed, the treatment resulted in an improvement in the hydrophobicity of the veneers due to the decrease in hemicellulose content. Notably, the reduction in strength and stiffness caused by the loss of cell wall polymers was not statistically significant. The treatment was successful in softening the wood material, reducing roughness, and increasing wettability. Despite a minimum of 20% reduction in glue line tension, the samples still surpassed the 1 MPa mark, showing satisfactory results. This demonstrates the feasibility of adjusting treatment variables to ensure the proper use of this adhesive.
An Assessment of the Impact of the Mining Industry on Soil and Plant Contamination by Potentially Toxic Elements in Boreal Forests
Authors: Gololobova Anna , Legostaeva Yana
Published: August 2023

This study was conducted in the territory of the industrial site of the Udachny Mining and Processing Division (Yakutia, northeast Russia). The objects of study were permafrost soils and two species of shrubs (Betula middendorffii T. and Duschekia fruticose R.). Soil and plant samples were analyzed using atomic absorption spectrometry for the presence of PTEs (Pb, Ni, Mn, Cd, Co, Cr, Zn, Cu, and As). The bioaccumulation factor (BAF), frequency of occurrence (Hi), pollution index (PI), and pollution load index (PLI) were calculated. The PI and PLI are calculated for both soil and two plant species for the first time in this study. The results showed that the soils have a high Ni, Cr, Co, As, and Mn content. It has been established that high soil pollution naturally leads to an increase in the concentration of elements in the leaves of shrubs. The soils and vegetation are dominated by elements associated with trap magmatism—Cr, Co, Cu, and dolerite dikes—Mn and Zn. For Betula middendorffii, the PLI was classified as unpolluted to moderately polluted, and Duschekia fruticosa. was classified as unpolluted. The high level of contamination is typical for areas located near industrial sites, such as waste dumps, kimberlite pipes, tailings ponds, and roads. The BAF results confirmed that the leaves of Betula middendorffii are able to accumulate more PTEs and have the highest level of resistance to PTE contamination in mining environments. This analysis showed that the consistent application of the PI, PLI, and BAF indices is very efficient in the ecological and biogeochemical assessment of the situation in industrial development areas.
Characterization of a Metallic-Ions-Independent L-Arabinose Isomerase from Endophytic Bacillus amyloliquefaciens for Production of D-Tagatose as a Functional Sweetener
Authors: Shehata Hoda M. , Abd El-Ghany Mohamed N. , Hamdi Salwa A. , Abomughaid Mosleh M. , Ghaleb Khaled I. , Kamel Zeinat , Farahat Mohamed G.
Published: August 2023

D-Tagatose is a low-calorie sugar substitute that has gained increased attention as a functional sweetener owing to its nutraceutical and prebiotic properties. Traditionally, D-tagatose is produced via the enzymatic conversion of L-galactose to D-tagatose by L-arabinose isomerase (L-AI). Nonetheless, the most reported L-AI enzymes are ion-dependent enzymes requiring Mn2+ and/or Co2+ as cofactors for their reactions, which limits their application due to safety and health concerns. Herein, we addressed the facile bioconversion of L-galactose to D-tagatose using a novel recombinant metallic-ions-independent L-AI derived from endophytic Bacillus amyloliquefaciens CAAI isolated from cantaloupe fruits. The ORF (1500 bp) of the L-arabinose isomerase gene (araA) was cloned and over-expressed in Escherichia coli. The recombinant enzyme (BAAI) was purified to homogeneity using Ni-NTA affinity chromatography, yielding a single distinct band with an apparent molecular mass of approximately 59 kDa as deduced from SDS-PAGE analysis. The purified enzyme showed optimum activity at pH and temperature of 7.5 and 45 °C, respectively, with obvious enzymatic activity in the presence of ethylenediaminetetraacetic acid (EDTA), indicating the metallic-ions independence from BAAI. The Km values of BAAI for D-galactose and L-arabinose were 251.6 mM and 92.8 mM, respectively. The catalytic efficiency (kcat/Km) values for D-galactose and L-arabinose were found to be 2.34 and 46.85 mM–1 min–1, respectively. The results revealed the production of 47.2 g/L D-tagatose from D-galactose (100 g/L) with 47.2% bioconversion efficiency in a metallic-ions-free reaction system that could be implemented in safe-production of food-grade low-calorie sweetener, D-tagatose.
Changes in the Concentration of Amino Acids and Bacterial Community in the Rumen When Feeding Artemisia absinthium and Cobalt Chloride
Authors: Ryazanov Vitaly , Tarasova Ekaterina , Duskaev Galimzhan , Kolpakov Vladimir , Miroshnikov Ivan
Published: August 2023

The aim of the study was to analyze the effect of a plant feed additive based on Artemisia absinthium and the metal trace element CoCl2 (cobalt chloride II) on the metabolism of amino acids closely related to the energy of feed and the bacterial community of the rumen of Kazakh white-headed bulls. Animals were divided into four groups: (A)—the control group of animals received the basic diet (BD), (B)—animals of the experimental group I were additionally given A. absinthium at a dose of 2.0 g/kg of dry matter (DM), (C)—II experimental group A. absinthium at a dose of 2.0 g/kg DM with additional CoCl2 (1.5 mg/kg/DM), and (D)—III experimental group was given only CoCl2 (1.5 mg/kg/DM) to study the rumen metabolism of amino acids and bacterial diversity of animals, rumen cannula were installed, the experiment was carried out using a 4 × 4 Latin square. It was found that additional feeding of A. absinthium, both separately and in combination with CoCl2, led to a change in the indices of the alpha biodiversity of the bacterial community. Correlation analysis revealed a linear relationship between the concentration of amino acids and the rumen bacterial community (p ≤ 0.05). The relationship between the values of amino acid concentrations and certain OTUs was established, with a possible percentile probability of 95% for the genera unclassified Lachnospiraceae, unclassified Clostridiales, unclassified Bacteroidales, Fibrobacter, Ihubacter, Phocaeicola, Paludibacter, Akkermansia, Vampirovibrio, unclassified Ruminococcaceae, and Alistipes. Thus, the use of A. absinthium and CoCl2 as feed additives, both in combination and without, leads to a change in the taxonomic structure affecting the concentration of amino acids. However, further research is needed to better understand the effectiveness and safety of these supplements.
Non-Saccharomyces Yeast Strains, Aromatic Compounds and Sensory Analysis of Italy and Negra Criolla Pisco from the Moquegua Region of Peru
Authors: Napa-Almeyda César Augusto , Criado Celia , Mayta-Hancco Jhony , Silva-Jaimes Marcial , Condezo-Hoyos Luis , Pozo-Bayón María Ángeles
Published: August 2023

In this study, we aimed to isolate and phenotypically characterize non-Saccharomyces yeast strains (NSYSs) from the skin of aromatic (Italy) and non-aromatic (Negra Criolla) grapes from vineyards in Moquegua, Peru, typically used for the production of pisco. Our second objective was to characterize the volatile compounds and sensory attributes of pisco made from these grapes. Pichia terricola (56%), Metschnikowia pulcherrima (31%), and Naganishia vaughanmartiniae (13%) were the main NSYSs isolated from the skin of aromatic Italy grapes and identified; meanwhile, Vishniacozyma carnescens (50%), Vishniacozyma heimaeyensis (30%), and Aureobasidium pullulans (20%) were identified on the skin of the non-aromatic Negra Criolla grapes. These NSYSs showed different capacities in terms of carbohydrate fermentation, polygalacturonase activity, ethanol tolerance, sulphite production, and nitrogen consumption. Moreover, the pisco resulting from these varieties of grape had different volatile profiles. Terpene alcohols such as citronellol, geraniol, linalool, and nerol were found in pisco made from Italy grapes, while higher contents of 2-phenylacetate and ethyl esters were found in Negra Criolla Pisco. Intermediate levels of both 1-hexanol and bencyl alcohol were also found in all the pisco. Sensory analysis performed by a trained pisco tasting panel showed that citric, floral, alcohol, and syrup descriptors were more marked in Italy Pisco, while nuts, syrup, alcohol, and floral were the most intense attributes of Negra Criolla Pisco. These results will contribute to determining the potential of indigenous grape yeasts from the Moquegua region as fermentation starters to improve the typical sensory qualities of the pisco produced in this region, which deserves further study.
The Effect of Yeast Inoculation Methods on the Metabolite Composition of Sauvignon Blanc Wines
Authors: Pinu Farhana R. , Stuart Lily , Topal Taylan , Albright Abby , Martin Damian , Grose Claire
Published: August 2023

Evidence from the literature suggests that different inoculation strategies using either active dry yeast (ADY) or freshly prepared yeast cultures affect wine yeast performance, thus altering biomass and many primary and secondary metabolites produced during fermentation. Here, we investigated how different inoculation methods changed the fermentation behaviour and metabolism of a commercial wine yeast. Using a commercial Sauvignon blanc (SB) grape juice, fermentation was carried out with two different inoculum preparation protocols using Saccharomyces cerevisiae X5: rehydration of commercial ADY and preparation of pre-inoculum in a rich laboratory medium. We also determined the effect of different numbers of yeast cells inoculation (varying from 1 × 106 to 1 × 1012) and successive inoculation on fermentation and end-product formation. The yeast inoculation method and number of cells significantly affected the fermentation time. Principal component analysis (PCA) using 60 wine metabolites showed a separation pattern between wines produced from the two inoculation methods. Inoculation methods influenced the production of amino acids and different aroma compounds, including ethyl and acetate esters. Varietal thiols, 3-mercaptohexanol (3MH), and 4-methyl-4-mercaptopentan-2-one (4MMP) in the wines were affected by the inoculation methods and numbers of inoculated cells, while little impact was observed on 3-mercaptohexyl acetate (3MHA) production. Pathway analysis using these quantified metabolites allowed us to identify the most significant pathways, most of which were related to central carbon metabolism, particularly metabolic pathways involving nitrogen and sulphur metabolism. Altogether, these results suggest that inoculation method and number of inoculated cells should be considered in the production of different wine styles.
Isolation and Characterization of Enterococcus faecium from Fermented Korean Soybean Paste with Antibacterial Effects
Authors: Han Kiseok , Park Soyoung , Sathiyaseelan Anbazhagan , Wang Myeong-Hyeon
Published: August 2023

In the present study, a total of eight Enterococcus faecium (OQ940301, OQ940302, OQ940303, OQ940304, OQ940305, OQ940308, OQ940309, and OQ940310) were isolated from soybean paste, a traditional Korean fermented food, and evaluated for their probiotic properties. The results showed that all the E. faecium strains survived in simulated human gastrointestinal conditions (4.1–5.59 log10 CFU/mL). In addition, the range of auto-aggregation was 5–25%, the hydrophobicity was around 94%, and it exhibited significant co-aggregation ability with Salmonella enterica and Staphylococcus aureus. However, all the isolates were shown to be resistant to Gentamycin. The bacterial cell-free supernatant showed antibacterial activity against S. enterica, Escherichia coli, Bacillus cereus, Listeria monocytogenes, and S. aureus. Furthermore, E. faecium exhibited potent anti-oxidant activity by scavenging 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radicals. In addition, safety was confirmed by evaluating the hemolytic activity in blood agar; none of the bacterial isolates showed hemolysis. These results demonstrated that E. faecium (OQ940301 and OQ940309) isolated from soybean paste showed a higher probiotic potential.
A Flame-Detection Algorithm Using the Improved YOLOv5
Authors: Xie Xingang , Chen Ke , Guo Yiran , Tan Botao , Chen Lumeng , Huang Min
Published: August 2023

Flame recognition is an important technique in firefighting, but existing image flame-detection methods are slow, low in accuracy, and cannot accurately identify small flame areas. Current detection technology struggles to satisfy the real-time detection requirements of firefighting drones at fire scenes. To improve this situation, we developed a YOLOv5-based real-time flame-detection algorithm. This algorithm can detect flames quickly and accurately. The main improvements are: (1) The embedded coordinate attention mechanism helps the model more precisely find and detect the target of interest. (2) We advanced the detection layer for small targets to enhance the model’s associated identification ability. (3) We introduced a novel loss function, α-IoU, and improved the accuracy of the regression results. (4) We combined the model with transfer learning to improve its accuracy. The experimental results indicate that the enhanced YOLOv5′s mAP can reach 96.6%, 5.4% higher than the original. The model needed 0.0177 s to identify a single image, demonstrating its efficiency. In summary, the enhanced YOLOv5 network model’s overall efficiency is superior to that of the original algorithm and existing mainstream identification approaches.
Development of a Multiplex Polymerase Chain Reaction Method for Rapid and Accurate Identification of Girella punctata and G. leonina (Teleostei: Girellidae)
Authors: Kim Eun-Mi , Lee Mi-Nan , Dong Chun-Mae , Noh Jae-Koo , Noh Eun-Soo , Kim Woo-Jin , Nam Bo-Hye , Kim Young-Ok
Published: August 2023

Girella punctata and Girella leonina are economically important species found in the East Sea; along the southern coast of Korea; south of Hokkaido, Japan; around Taiwan; and in the East China sea. In Korea, these two species hold high value, particularly on Jeju Island. These species have similar appearances, and it is challenging to distinguish them, particularly during the seed period. We detected genetic differences in the mtDNA (COI gene) of G. punctata and G. leonina, which are morphologically indistinguishable, and developed species-specific genetic markers for their identification. In total, 16 and 4 haplotypes of the COI genes were obtained from G. punctata (n = 164) and G. leonina (n = 36), respectively. The haplotype diversity (Hd) and nucleotide diversity (Pi, %) of the COI were 0.359 and 0.054 for G. punctata and 0.560 and 0.078 for G. leonina, respectively. We designed a Girella species common primer (control) and species-specific primer sets (experimental) for the two species. Amplicon sizes of 991, 579, and 391 bp were obtained for common primers of the two Girella species G. punctata and G. leonina. To confirm multiple targets in a single reaction, multiplex PCR conditions were optimized to adjust its resolution and accuracy. The detection levels of the multiplex PCR were confirmed to be 0.01 ng/µL for the two Girella species. The multiplex PCR was not associated with cross-reactivity between G. punctata and G. leonina. This multiplex species-specific PCR method provides a simple and rapid technique for the identification of two Girella species, thus increasing the efficiency and quality of Girella species stock management and forensic identification to prevent species misidentification.
Molecular Cloning and Gene Expression of Type I Suppressors of Cytokine Signaling 6 and 7 (SOCS6 and SOCS7) in Whiteleg Shrimp (Litopenaeus vannamei)
Authors: Wikumpriya Gunasekara Chathura , Prabhatha Madhuranga Walawedurage Srinith , Lee Jiye , Kim Chan-Hee
Published: August 2023

Suppressor of cytokine signaling (SOCS) genes are essential negative regulators that modulate cytokine signaling and play key roles in numerous biological processes, including immune responses. In this study, we cloned the complementary DNA (cDNA) sequences of two SOCS genes, designated as LvSOCS6 and LvSOCS7, from the whiteleg shrimp, Litopenaeus vannamei. LvSOCS6 encoded a polypeptide of 463 amino acids (aas), spanning 1392 base pairs (bps), while LvSOCS7 encoded a significantly larger polypeptide of 955 aas, encompassing 2868 bps. Both LvSOCS proteins exhibited conserved domains associated with SOCS, including a centralized Src homology 2 (SH2) domain and a C-terminal SOCS box. Phylogenetic analysis revealed that the deduced aa sequences of LvSOCS6 and LvSOCS7 clustered within the invertebrate type I SOCS family, indicating their evolutionary relatedness. Tissue distribution analysis demonstrated ubiquitous expression of both LvSOCS genes across all examined tissues, with LvSOCS6 showing heightened expression in the gills and LvSOCS7 in the gills and stomach. Notably, mRNA expression patterns of LvSOCS genes following LPS and poly (I:C) stimulations exhibited significant upregulations, while PGN stimulation yielded incongruous results across the examined tissues. Interestingly, concurrent with the diminished expression of LvSOCS6 and LvSOCS7, there was significant elevation in mRNA expression levels of LvSTAT, a vital component of the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. These findings shed light on the potential involvement of the newly discovered type I SOCS genes in regulating the JAK/STAT pathways and playing pivotal roles in orchestrating the innate immune responses in L. vannamei defense mechanisms.
Sustainable Preparation Behavior for Kitchen Staff in Order to Limit Food Waste
Authors: Lu Min-Yen , Ko Wen-Hwa
Published: August 2023

The concepts of culinary sustainability and avoiding the waste of surplus food have become important sustainability trends today. How the handling of surplus food can be integrated into the catering industry is a topic of concern in the industry. Kitchen staff are the vital soul of any restaurant, and we intend to discuss how kitchen staff actually behave and explore factors that influence their behaviors in order to develop an implementation model for food waste prevention. Therefore, this study explored a model of ethical sustainability, professional competence, self-efficacy, sustainable food preparation objectives, and sustainable food promotion and behavior focused on limiting food waste, using structural equation modeling (SEM) to understand the relationship between various constructs. This study used a questionnaire and surveyed employees who had been employed for more than 6 months in Taiwan. From May to August 2022, 500 questionnaires were distributed; 415 valid questionnaires were retrieved, yielding a 90.2% recovery rate. According to the structural equation modeling analysis between the dimensions, ethical sustainability should have a positive influence on professional competence in food waste prevention and self-efficacy. Professional competence in food waste prevention affected self-efficacy and behavioral intentions during food preparation; self-efficacy also significantly affected behavioral intentions towards sustainable food preparation. Similarly, behavioral intention had a positive influence on promoting sustainable behaviors. There is a significant relationship between all constructs in this study. Professional competence in food waste prevention was found to be the mediating factor between ethical sustainability and behavioral intentions toward sustainable food preparation, and self-efficacy was the mediating factor between professional competence in food waste prevention and behavioral intentions toward sustainable food preparation.
Bioactive Potential of Aqueous Phenolic Extracts of Spices for Their Use in the Food Industry—A Systematic Review
Authors: Duque-Soto Carmen , Ruiz-Vargas Ana , Rueda-Robles Ascensión , Quirantes-Piné Rosa , Borrás-Linares Isabel , Lozano-Sánchez Jesús
Published: August 2023

The interest on the use of natural sources in the food industry has promoted the study of plants’ phenolic compounds as potential additives. However, the literature has been focusing on essential oils, with very few studies published regarding aqueous extracts, their phenolic composition, and bioactivity. A systematic review was conducted on different databases following PRISMA guidelines to evaluate the relevance of the phenolic content of different aromatic spices (oregano, rosemary, thyme, ginger, clove, and pepper), as related to their bioactivity and potential application as food additives. Although different extraction methods have been applied in the literature, the use of green approaches using ethanol and deep eutectic solvents has increased, leading to the development of products more apt for human consumption. The studied plants present an interesting phenolic profile, ranging from phenolic acids to flavonoids, establishing a correlation between their phenolic content and bioactivity. In this sense, results have proven to be very promising, presenting those extracts as having similar if not higher bioactivity than synthetic additives already in use, with associated health concerns. Nevertheless, the study of spices’ phenolic extracts is somehow limited to in vitro studies. Therefore, research in food matrices is needed for more understanding of factors interfering with their preservation activity.
Future Perspective and Technological Innovation in Cheese Making Using Artichoke (Cynara scolymus) as Vegetable Rennet: A Review
Authors: Bravo Bolívar Michael Steven , Pasini Federica , Marzocchi Silvia , Ravagli Cesare , Tedeschi Paola
Published: August 2023

Milk coagulation is a process used for the formulation of different dairy products such as cheese. In this process, milk undergoes changes in its chemical stability thanks to acidification or enzymatic reactions. Traditionally, milk coagulation has been carried out with rennet of animal origin, but recently, the research of new types of rennet such as microbial rennet and vegetable rennet has increased. This study aims to present an organized review of the most relevant information on lactic coagulation, its relationship with vegetable rennets, and the importance of the botanical genus Cynara in the extraction of vegetable rennets, focusing on the coagulant potential of artichoke (Cynara scolymus). We conducted this literature review and found that lactic coagulation and vegetable rennets are linked through the enzymatic activity of the latter. The results of the main studies demonstrated a strong relationship between vegetable rennets and protease enzymes as well as the presence of these enzymes in extracts of cardoon (Cynara scolymus) and artichoke (Cynara scolymus). In addition, studies highlight the presence of thistle extracts in artisanal cheese preparations in the Iberian Peninsula. Based on the results of the studies, a comparison between cheeses made with vegetable rennet and those made with traditional rennet was also carried out. Although the results show that the use of vegetable rennet in the manufacture of cheese can confer undesirable characteristics, the use of extracts from Cynara plants demonstrates that vegetable rennets have an industrial potential, especially the one obtained from artichoke (Cynara scolymus) due to its high availability. Nevertheless, specific studies are required for a better understanding and application of this rennet.
Prevalence and Types of Extended-Spectrum β-Lactamase-Producing Bacteria in Retail Seafood
Authors: Pearce Ryan , Conrady Beate , Guardabassi Luca
Published: August 2023

Objectives: To assess prevalence and types of extended-spectrum β-lactamase (ESBL)-producing bacteria in retail seafood. Methods: A literature review was completed according to international guidelines for systematic reviews, except for being performed by a single reviewer. Kruskal–Wallis and Dunn tests were used to determine statistical differences between continents or seafood types. Results: Among 12,277 hits, 42 publications from 2011 to 2023 were deemed relevant to the review’s objectives. The median prevalence of ESBL-contaminated products was 19.4%. A significantly lower prevalence was observed in Europe (p = 0.006) and Africa (p = 0.004) compared to Asia. Amongst the 2053 isolates analyzed in the selected studies, 44.8% were ESBL-positive. The predominant type was CTX-M (93.6%), followed by TEM (6.7%) and SHV (5.0%). Only 32.6% and 18.5% of the CTX-M-positive isolates were typed to group and gene level, respectively. While group 1 (60.2%) was prevalent over group 9 (39.8%) among Enterobacterales, the opposite trend was observed in Vibrio spp. (60.0% vs. 40.0%). Information at gene level was limited to Enterobacterales, where CTX-M-15 was the most prevalent (79.2%). Conclusions: On average, one in five seafood products sold at retail globally is contaminated with ESBL-producing Enterobacterales of clinical relevance. Our findings highlight a potential risk for consumers of raw seafood, especially in Asia.
Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FRT7 from Chinese Paocai
Authors: Cai Hongying , Li Xuan , Li Daojie , Liu Weiwei , Han Yunsheng , Xu Xin , Yang Peilong , Meng Kun
Published: August 2023

Gamma-aminobutyric acid (GABA) is a widely available non-protein amino acid whose physiological importance goes beyond its role as an inhibitory neurotransmitter in mammals. The GABA synthesis ability of ten strains of Lactiplantibacillus plantarum was screened. They produced GABA ranging from 48.19 ± 3.44 to 100.75 ± 1.63 mg/L at 24 h-cultivation. Among them, Lp. plantarum FRT7 showed the highest GABA production. Therefore, FRT7 was chosen for GABA yield optimization. A one-factor-at-a-time strategy analysis of the GABA yield of FRT7 was performed, including the culture temperature, incubation time, inoculum volume, initial pH, the initial amount of monosodium glutamate (MSG), and pyridoxal 5’-phosphate (PLP) concentration, based on which the response surface methodology (RSM) was performed. After being cultured in an MRS culture medium supplemented with 3% MSG and 2 mmol/L of PLP at 40 °C with an initial pH of 7.0 for 48 h, the GABA reached a maximum yield of 1158.6 ± 21.22 mg/L. The results showed the experimental value of the GABA yield was in good agreement with the predicted values. Furthermore, the results from the RSM also indicated that the initial MSG addition, PLP concentration, and incubation time were significant variables. These results suggest that Lp. plantarum FRT7 has the potential to be a health-beneficial probiotic with commercial capabilities.
Relaunching a Traditional Durum Wheat Product: New Cultivars and Introgression Lines Identified for Frike Making in Turkey
Authors: Özberk Fethiye , Martínez-Moreno Fernando , Kuzmanović Ljiljana , Ceoloni Carla , Özberk İrfan
Published: August 2023

Frike is an ancient and traditional food product prepared from early harvested whole wheat grain, particularly durum wheat (DW). Due to its many health beneficial effects, frike is considered a functional food. It is also a lucrative commodity, produced in various West Asian and North African countries and typically in Southeastern Turkey. However, no systematic assessment of the most-suitable genotypes for frike production in the region is available. This study aimed to carry out such an evaluation, based on frike yield, quality traits, marketing price, and profitability, on a set of 20 DW cultivars and DW-Thinopyrum ponticum introgression lines (ILs). The results based on a field trial performed in Gölbaşı (Adıyaman, Turkey) in the 2021–2022 season revealed the Turkish varieties Tüten-2002, Edessa, Artuklu, and Perre, together with the R5 IL to have the highest frike yields measured on 3 kg of roasted fresh spikes. The highest marketing prices were obtained by Turkish varieties Sariçanak-98, Burgos, Sümerli, and Artuklu, along with the R112 IL, excelling in quality traits. Considering all parameters, the Turkish cultivars Artuklu, Firat-93, and Sariçanak-98, besides the R112 IL, resulted in being the most-convenient genotypes for frike making, thus representing good candidates for maintaining cultural and genetic diversity in food production from a staple crop such as DW.
Metal Load of Potentially Toxic Elements in Tuna (Thunnus albacares)—Food Safety Aspects
Authors: Lehel József , Papp Zita , Bartha András , Palotás Péter , Szabó Rita , Budai Péter , Süth Miklós
Published: August 2023

The consumption of marine fishes has a positive effect on a consumer’s health; however, it poses a potential risk due to their level of heavy metals in their body. Heavy metals can be naturally found in the environment, but their concentration can be increased with anthropogenic activities. Samples of tuna (Thunnus albacares) were collected at a fishery market. The potentially toxic elements (arsenic, cadmium, lead, and mercury) were determined with a validated method in the flesh of fish using inductively plasma optical emission spectrometry after microwave digestion. Generally, the average concentration of them was below the official limit values regulated by the European Union, except for lead. Based on the concentrations of arsenic (inorganic derivates: 0.05 ± 0.02 mg/kg) and cadmium (0.03 ± 0.01 mg/kg) in the tuna fish samples, and their calculated EDI values (As: 0.03–0.09 µg/kg/day; Cd: 0.05–0.07 µg/kg/day), the investigated food could be declared safe for human consumption. Generally, mercury content was below the official regulated limit, and the calculated EDI value was below the dietary reference value (0.3 μg/kg/day) in most of the samples (90%), exceeding it only in two samples (0.69 and 0.82 μg/kg/day); thus, they may not be harmful to the consumer. The concentration of lead above the official maximum limit (0.30 mg/kg) in 40% of tuna samples (0.30–1.59 mg/kg), as well as the exceeding of the dietary reference value for lead (adult: 0.16 μg/kg/day; children: 0.26 μg/kg/day) based on the calculated EDI values (0.28–1.49 μg/kg/day), draw attention to the importance of environmental pollution and the protection of consumers’ health.
Use of X-Irradiations in Reducing the Waste of Aflatoxin-Contaminated Pistachios and Evaluation of the Physicochemical Properties of the Irradiated Product
Authors: Hojjati Mohammad , Shahbazi Samira , Askari Hamed , Makari Mina
Published: August 2023

This study investigates the effects of electron beam irradiation (0, 1, 2, 4, and 6 kGy) on Aspergillus flavus, aflatoxin B1 (AFB1), and the physicochemical properties of pistachios. The findings suggested that e-beam significantly reduced the spore population of A. flavus and the concentration of AFB1 at doses of 4 and 6 kGy. Three AFB1 degradation products were detected via LC-MS analysis and their structures were presented. Total phenolic content was improved at a dose of 2 kGy, while antioxidant activity was decreased in all treatments in both DPPH and ABTS assays. The chlorophyll and carotenoid content declined and the color indices changed, leading to a darker color. E-beam at a dose of 2 kGy raised the soluble protein levels and changed the intensity and pattern of protein bands. Irradiation doses of up to 6 kGy enhanced the content of malondialdehyde and total saturated fatty acids while leading to a decline in unsaturated fatty acids. The quality features were adversely affected at doses > 4 kGy. The findings suggest that as an alternative method, e-beam at doses ≥ 2 kGy can effectively decrease fungal load and aflatoxin B1 contamination, and e-beam application at doses ≤ 2 kGy can maintain the physicochemical attributes of pistachios to an acceptable extent.
Influence of Post-Heating Treatment on the Sensory and Textural Properties of Stirred Fermented Milk
Authors: Gao Fei , Li Dongdong , Li Hongliang , Chen Han , Mao Xueying , Wang Pengjie
Published: August 2023

The purpose of this study was to investigate the post-heating induced changes in the textural and sensory characteristics of stirred fermented milk. The textural and rheological properties of post-heating fermented milk (55–85 °C, 25 s) with respect to viscoelastic behaviors, viscosity, textural parameters, etc., were monitored, and the friction behaviors and sensory attributes were assessed. Treatments below 65 °C/25 s increased the textural properties of fermented milk such as gel strength, firmness, and viscosity, due to the moderate aggregation and increased linkages of microgels. In this case, despite the size and amount their aggregates increased (~15–~21 μm), they exhibited similar frictional behaviors and sensory attributes. However, treatments above 65 °C/25 s degraded textural properties due to excessive aggregation (~46–~63 μm), accompanied by unacceptable grainy attributes, which could be characterized by their good correlations with tribological coefficients and particle size parameters. These findings could provide an understanding of the quality formation of post-heating fermented milk and a perspective to improve the textural defects of ambient fermented milk products.
Motivational Drivers behind the Consumption of Dietary Supplements by Leisure-Time Athletes
Authors: Kovács Ildikó , Liska Fanny , Veres Zoltán
Published: August 2023

The prevalence of dietary supplement use has been growing continuously worldwide. Nevertheless, limited research evidence is available on dietary supplement usage related to the segment of leisure-time athletes with the determinants of motivational drivers, sport types, and demographic characteristics. This research, which was conducted in Hungary among leisure-time athletes, aims to learn about the demographic and sports characteristics of consumers who identified themselves as active dietary supplement consumers in the survey. The motivational drivers for consuming dietary supplements and their differences, as well as the frequency of dietary supplement consumption in each sports category, are examined. The findings of the study provide valuable insights into the key motivational drivers, among which health preservation, improvement in physical well-being, and cartilage protection are predominant. The results show that there are significant differences between segments taking part in different leisure-time sport activities and age groups when it comes to dietary supplement usage. Three different segments were identified based on the motivational drivers examined. As a limitation, to note is that due to the sample size, the study can be considered as prospective. In practical terms, the results of the study can be used to support marketing projects that aim to reach leisure-time sport athletes.
Effect of Combined Infrared Hot Air Drying on Yam Slices: Drying Kinetics, Energy Consumption, Microstructure, and Nutrient Composition
Authors: Zhang Jikai , Zheng Xia , Xiao Hongwei , Li Yican , Yang Taoqing
Published: August 2023

Using hot air drying (HAD) and combined infrared hot air drying (IR-HAD) test devices, the drying kinetics, unit energy consumption, color difference values, rehydration rate, microstructure, and changes in polysaccharide and allantoin contents of yam slices were examined at various temperatures (50 °C, 55 °C, 60 °C, 65 °C, and 70 °C). The findings demonstrated that each of the aforementioned parameters was significantly impacted by the drying temperature. IR-HAD dries quicker and takes less time to dry than HAD. The Deff of IR-HAD is higher than that of HAD at the same temperature and increases with the increase in temperature. The activation energy required for IR-HAD (26.35 kJ/mol) is lower than that required for HAD (32.53 kJ/mol). HAD uses more energy per unit than IR-HAD by a factor of greater than 1.3. Yam slices treated with IR-HAD had higher microscopic porosity, better rehydration, lower color difference values, and higher polysaccharide and allantoin levels than HAD-treated yam slices. The IR-HAD at 60 °C had the greatest comprehensive rating after a thorough analysis of the dried yam slices using the coefficient of variation method. Three statistical indicators were used to evaluate six thin-layer drying models, and the Weibull model was most applicable to describe the variation of drying characteristics of yam slices.