bluuebunny/crossref_metadata_2025 huggingface dataset:
High-speed observation of bending fracture process of carbon fiber reinforced plastic composite/Nomex™ honeycomb sandwich panel
Authors: Ogasawara Toshio , Yoshinaga Hajime , Oiwa Mae , Oguri Tsuyoshi , Matsuda Takamasa , Kusano Hideaki , Yoshimura Akinori
Published: February 2020

Failure mechanism of sandwich panels is very complicated. The objective of this study is to demonstrate the usefulness of high-speed observation to elucidate the failure mechanism of sandwich panels. Failure processes of carbon fiber reinforced plastic composite/Nomex™ honeycomb sandwich panels were observed using two synchronized high-speed cameras with an 8 µs frame rate. The sandwich panel was subjected to a four-point bending load in accordance with ASTM-D7249. Digital image correlation was also adopted for measuring the surface strain. Results show fracture initiation: (1) local (intra-cell or wrinkling) buckling of the face sheet at the edge (Type I and III), or (2) local buckling (intra-cell or wrinkling) of the face sheet at the middle of the specimen (Type II). Following local buckling of the face sheet, debonding between the face sheet and the honeycomb core occurred within 10–20 µs. Subsequently, crack propagation of the face sheet occurred, leading to final failure of the sandwich panel. It was about 150–200 µs from the local buckling of face sheet to the final failure of sandwich panel. Results demonstrated that high-speed observations are effective to elucidate failure mechanisms of the sandwich panel subjected to a bending load.
Combined central retinal vein occlusion and branch retinal artery occlusion treated with intravitreal dexamethasone implant: A case report
Authors: Arrigo Alessandro , Knutsson Karl Anders , Rajabjan Firuzeh , Augustin Victor A , Bandello Francesco , Parodi Maurizio Battaglia
Published: February 2020

Purpose:

To report a case of combined central retinal vein occlusion and branch retinal artery occlusion in a 51-year-old male with a very good response to dexamethasone implant therapy.

Methods:

This is a descriptive case report based on data from clinical records, patient observation and follow-ups, and analysis of acquired diagnostic tests.

Results:

A 51-year-old man presented with sudden vision loss and best-corrected visual acuity of 20/40 in his left eye. A pale inferotemporal arterial branch course area along with increased vascular tortuosity, retinal hemorrhages, optic disk swelling, and macular edema were observed on slit lamp biomicroscopy examination. Right eye was normal. Diagnosis of combined central retinal vein occlusion and branch retinal artery occlusion in left eye was confirmed by fluorescein angiography and color fundoscopy, respectively. Optical coherence tomography confirmed subretinal fluid and intraretinal cysts with a prominent middle-limiting membrane in the inner synaptic portion of the outer plexiform layer, corresponding to areas of paracentral acute middle maculopathy. Intravitreal dexamethasone implant was administered to the patient. One month later, visual acuity was recovered with complete absorption of macular edema. Functional and anatomical stabilization were confirmed after 24 months.

Conclusion:

Combined central retinal vein occlusion and branch retinal artery occlusion represents a rare condition, with variable functional outcomes due to the long-term complications such as macular edema. We hypothesize that prompt diagnosis and immediate intravitreal corticosteroid implant therapy reduced macular edema, thus contributing to arterial perfusion improvement, which in this case lead to a full sustainable recovery with limited functional and anatomical damage.
A miRNA- and mRNA-seq-Based Feature Selection Approach for Kidney Cancer Biomakers
Authors: Kim Shinuk
Published: February 2020

Microarray data sets have been used for predicting cancer biomarkers. Yet, replication of the prediction has not been fully satisfied. Recently, new data sets called deep sequencing data sets have been generated, with an advantage of less noise in computational analysis. In this study, we analyzed the kidney miRNA and mRNA sequence data sets for predicting cancer markers using 5 different statistical feature selection methods. In the results, we obtained 3 mRNA- and 27 miRNA-based cancer biomarkers to compare with the normal samples. In addition, we clustered the kidney cancer subtypes using a nonnegative matrix factorization method and obtained significant results of survival analysis from the 2 separate groups including miRNA-342 and its target eukaryotic translation initiation factor 5A ( EIF5A).
Global Mental Health: Interdisciplinary challenges for a field in motion
Authors: Bemme Dörte , Kirmayer Laurence J
Published: February 2020

In recent years, efforts in Global Mental Health (GMH) have evolved alongside critical engagement with the field's claims and interventions. GMH has shifted its agenda and epistemological underpinnings, increased its evidence base, and joined other global policy platforms such as the Sustainable Development Goals. This editorial introduction to a thematic issue traces the recent shifts in the GMH agenda and discusses the changing construct of “mental health” as GMH moves away from a categorical biomedical model toward dimensional and transdiagnostic approaches and embraces digital technologies. We highlight persistent and emerging lines of inquiry and advocate for meaningful interdisciplinary engagement. Taken together, the articles in this special issue of Transcultural Psychiatry provide a snapshot of current interdisciplinary work in GMH that considers the socio-cultural and historical dimensions of mental health important and proposes reflexive development of interventions and implementation strategies.
An ethnic-group comparison of caregiver beliefs about early psychotic illness in a UK sample: Implications for evidence-based caregiver interventions
Authors: Smith Lindsay M , Onwumere Juliana , Craig Tom KJ , Kuipers Elizabeth
Published: February 2020

This study aimed to investigate potential ethnic differences in cognitive responses to caregiving in psychosis that might be relevant to the delivery of caregiver interventions for difficulties related to psychosis. We compared cross-sectional outcomes across early-stage caregivers who self-identified as white British ( n = 37) and those who self-identified as black African or Caribbean ( n = 41) using United Kingdom (UK) census ethnicity criteria. Self-report questionnaires were used to examine caregiver beliefs about psychosis, including the perceived causes, consequences, timeline, the degree of control that patients have over their difficulties, as well as their appraisals of caregiving. Caregivers from black African or Caribbean backgrounds reported a significantly shorter expected duration of illness than white British caregivers. They were also more likely to cite psychosocial causes (e.g., relationship issues), and less likely to cite biological and genetic causes, as their principal explanations for problems. However, overall differences in perceived causes of illness between ethnicities were not significant, despite the power in this sample to detect medium-sized effects. Factors associated with ethnicity may contribute to individual differences in explanatory models of illness and in experiences of caregiving. A degree of sensitivity to the range of views that people might hold about psychosis may help to engage caregivers from diverse ethnic backgrounds.
Appearance of congenital hand anomalies
Authors: Nietosvaara Noora N. , Sommarhem Antti J. , Puhakka Jani M. , Tan Ruth E. S. , Schalamon Johannes , Nietosvaara Aarno Y.
Published: February 2020

Background and objective:

Impact of appearance of congenital hand anomalies has not previously been reported. The purpose of this study was to describe the common perception about how different congenitally malformed hands look.

Methods:

We developed a questionnaire in a game format to evaluate the appearance of different hands. Altogether 1450 (954 females) 4- to 84-year-old residents (296 children) of two European and one Asian (n = 102) country were asked to rate the appearance of different looking hands on a five-point pictorial Likert-type scale. Standardized photographs of the dorsal aspect of 17 different congenitally malformed non-operated hands and a normal hand were presented to respondents. Significance of age, gender, nationality, and profession of the respondents was assessed.

Results:

The respondents’ ranking order of the hands was nearly consistent. The normal hand (mean = 4.43, standard deviation = 0.85, Md = 5) and clinodactyly (mean = 4.37, standard deviation = 0.86, Md = 5) were perceived to have the best appearance. Symbrachydactyly (mean = 1.42, standard deviation = 0.68, Md = 1) and radial club hand (mean = 1.40, standard deviation = 0.68, Md = 1) received the lowest scores. Adults rated the appearance of hands higher than children regarding 14 hands, females higher than men regarding 15 hands, and Europeans higher than Asians in 4 hands (p < 0.05, respectively). Europeans rated four-finger hand (mean = 3.21, standard deviation = 1.18, Md = 3) better looking than six-finger hand (mean = 2.92, standard deviation = 1.18, Md = 3, p < 0.005), whereas Asians gave higher scores to six-finger hand (mean = 2.66, standard deviation = 1.26, Md = 3) compared to four-finger hand (mean = 2.51, standard deviation = 1.14, Md = 2). Medical doctors and nurses gave higher scores compared to the other profession groups, school children, and high school students in five hands (p < 0.05).

Conclusions:

A normal hand is perceived distinctly better looking than most congenitally different hands. Different malformations’ appearance was ranked very coherently in the same order despite of participants’ age, gender, nationality, or profession. Asians seem to prefer an additional digit to a four-finger hand.
De-colonizing public spaces in Malaysia: dating in Kuala Lumpur
Authors: Nawratek Krzysztof , Mehan Asma
Published: February 2020

This article discusses places and practices of young heterosexual Malaysian Muslims dating in non-private urban spaces. It is based on research conducted in Kuala Lumpur in two consecutive summers 2016 and 2017. Malaysian law (Khalwat law) does not allow for two unrelated people (where at least one of them is Muslim) of opposite sexes to be within ‘suspicious proximity’ of one another in public. This law significantly influences behaviors and activities in urban spaces in KL. In addition to the legal framework, the beliefs of Malaysian Muslims significantly influence the way they perceive space and how they behave in the city. The article discusses the empirical theme, beginning with the participants’ narratives of their engagement with the dominant sexual and gender order in non-private spaces of KL. Utilizing questionnaires, interviews and observations, this article draws upon a qualitative research project and questions the analytical usefulness of the notion of public space (as a Western construct) in the context of an Islamic, post-colonial, tropical, global city.
Medical Malpractice in Nerve Injury of the Upper Extremity
Authors: Krauss Emily M. , Shankar Vikram , Patterson Jennifer Megan Mackinnon , Mackinnon Susan E.
Published: February 2020

Background: Medical malpractice accounts for more than $55 billion of annual health care costs. Updated malpractice risk to surgeons and physicians related to upper extremity peripheral nerve injury has not been published. Methods: A comprehensive database analysis of upper extremity nerve injury claims between 1995 and 2014 in the United States was conducted using the Medical Professional Liability Association Data Sharing Project, representing 24 major insurance companies. Results: Nerve injury in the upper extremity accounted for 614 (0.3%) malpractice claims (total of 188 323). Common presenting diagnoses included carpal tunnel syndrome (41%), upper extremity fractures (19%), and traumatic nerve injuries to the shoulder or upper limb (8%). Improper performance (49% of total claims) and claims without evidence of medical error (19%) were the most common malpractice suits. Orthopedic surgeons were the most frequently targeted specialists (42%). In all, 65% of nerve injury claims originated from operative procedures in a hospital, 59% of claims were dismissed or withdrawn prior to trial, and 30% resulted in settlements. Thirty-three percent of claims resulted in an indemnity payment to an injured party, with an average payout of $203 592 per successful suit. Only 8% of claims resulted in a completed trial and verdict, and verdicts were overwhelmingly in favor of the defendant (83%). Conclusions: Most malpractice claims from peripheral nerve injuries in the United States arise from the management of common diagnoses, occur in the operating room, and allege improper performance. Strategies to reduce malpractice risk should emphasize the management of common conditions and patient-physician communication.
Optimal Position of the Suture Button Suspensionplasty (TightRope) for Thumb Basal Joint Arthritis
Authors: Hozack Bryan A. , Fram Brianna , Ilyas Asif M. , Rivlin Michael , Liss Frederic E. , Jones Christopher M.
Published: February 2020

Background: Surgical treatment of basal joint arthritis commonly consists of trapeziectomy followed by various suspensionplasty techniques to provide stability to the thumb ray. Our study goal was to assess the motion and stability of the thumb ray after trapeziectomy and placement of a suture button (Mini TightRope®, Arthrex, Naples, Florida) in a high- or low-angle trajectory. We hypothesized that a low-angle trajectory would yield the greatest stability while providing maximal motion of the thumb. Methods: Eleven fresh-frozen cadaver arms were imaged fluoroscopically in anterior-posterior and lateral views before and after trapeziectomy, and after placement of low- and high-angle suture buttons. The intermetacarpal angle between the thumb and index metacarpals was measured after application of a standard force. Radial abduction, opposition, subsidence, palmar abduction, adduction, and subsidence were measured. Results: Compared to posttrapeziectomy constructs, low- and high-angle TightRope constructs demonstrated less subsidence, low-angle TightRopes had less palmar abduction, and high-angle TightRope constructs had less radial abduction and adduction. High-angle TightRopes allowed more palmar abduction than low-angle constructs. The high-angle TightRopes trended toward more subsidence than low-angle constructs, although it was not significant. Conclusions: Both TightRope constructs provided improved axial stability after trapeziectomy while not excessively limiting any one motion of the thumb. Compared to the high-angle trajectory, the low-angle TightRope placement provided a more stable construct with respect to subsidence and angular motion. Given the concern for excessive motion of the first metacarpal base with the high-angle construct, we recommend a low-angle trajectory TightRope placement.
Screening for Potential Biomarkers in Peripheral Blood From Miners Exposed to Radon Radiation
Authors: Sun Lu , Pan Yan , Wang Xiaochun , Gao Gang , Wu Lina , Piao Chunnan , Ruan Jianlei , Liu Jianxiang
Published: February 2020

In this cohort study of 144 miners, 72 miners worked underground (the study group) and 72 miners worked aboveground (the control group). Based on questionnaire data and of radon concentration measurements, the cumulative radon exposure dose was calculated for each miner using the parameters recommended in International Commission on Radiological Protection Publication 137. Hematological parameters such as lymphocyte count (LYM) and neutrophil count (NE) were assessed, cell cycle phases and regulatory proteins were detected by flow cytometry, and microRNA (miRNA) microarray screening and real-time polymerase chain reaction (PCR) were used to detect miRNAs in plasma. The interrelationships between various potential biomarkers were analyzed using bioinformatics and statistical methods. The mean cumulative exposure dose of underground miners and controls was 982 and 48 mSv, respectively. Hematological parameters (such as LYM and NE) were significantly lower in the underground group. Cyclin-dependent kinase (CDK)-2, CDK4, CDK6, CyclinA2, CyclinD1, and CyclinE1 were significantly higher in the underground group. MicroRNA microarray screening showed that 5 miRNAs were downregulated (fold-change >2) in the underground group. The real-time PCR detection results of miR-19a, miR-30e, miR-335, and miR-451a were consistent with the screening results. LYM, NE, CDK2, CDK4, CDK6, Cyclin A2, Cyclin D1, Cyclin E1, miR-19a, miR-30e, miR-335, and miR451a are potential biomarkers of radon radiation damage.
Vascular deformation in human atherosclerotic carotid artery evaluated by 2D analysis of ultrasonography
Authors: Rafati Mehravar , Rafati-Rahimzadeh Mehrdad , Moladoust Hassan , Sehhati Mohammadreza
Published: February 2020

Objectives

Common carotid artery (CCA) remodelling in the atherosclerosis process is an inherent necessary element that decreases the progress of significant lumen compromise. The present study used a semi-automated method to assess relationships of intima-media thickness (IMT), lumen diameter (LD) and inter-adventitial diameter (IAD) using ultrasound B-mode images of atherosclerotic carotid artery.

Methods

In the cross-sectional study, 120 male subjects (age range: 40–60 years) were classified into four research groups namely control, mild, moderate, and severe stenosis. The maximum near and far wall IMT, mean of both walls’ IMT and IAD, and also LD of the left CCA were extracted for all participants. Pearson correlation coefficient was utilized to investigate relationships of IMT, LD, and IAD.

Results

Results revealed that the maximum far and near wall IMT, mean of both walls’ IMT and IAD in the CCA were significantly different in stenosis patients and the control group ( p< 0.001). However, there were no significant differences among the four studied groups in terms of LD of CCA ( p = 0.65). There was a stronger correlation between mean of both walls’ IMT and IAD in comparison with mean far wall IMT and IAD ( p < 0.001).

Conclusions

Results indicated that changes of IAD in the left CCA were associated with carotid deformation, and thus it can be considered as a predictor of atherosclerosis process.
Synthesis of N-substituted-4-methylene-oxazolidinones via base-catalyzed cyclization of propargylic alcohols with p-toluenesulfonyl isocyanate
Authors: Pi Shao-Feng , Guo Yue-Meng , Zhou Zheng-Rui , Sun Han-zhou , Yi Bing
Published: February 2020

A practical method is developed for the synthesis of oxazolidinone derivatives, an important class of heterocyclic compounds. The effect of bases and solvents on this cyclization reaction is discussed and a simple new base–solvent system (triethylamine in toluene) is found to be the most effective. The reaction conditions developed here are mild and no by-products are observed. Moreover, using optimized conditions, a number of differently substituted propargylic alcohols are cyclized to the corresponding N-substituted-4-methylene-oxazolidinones in yields of up to 99%.
The yellow vests and the communicative constitution of a protest movement
Authors: Clifton Jonathan , de la Broise Patrice
Published: February 2020

Contemporary protest movements are skeptical of mainstream media outlets, and so to communicate, they make extensive use of social media such as YouTube, Instagram and Twitter. Most research to date has considered how protest movements, as preexistent entities, use such social media to communicate with stakeholders, but little, if any research, has considered how a protest movement is constituted in and through communication. Using the Montreal School’s ventriloquial approach to communication and using YouTube video footage of the gilets jaunes – a contemporary French protest movement – in action, the purpose of this article is to explicate how a protest movement that resists the state’s authority is constituted in and through a textual artifact (a video clip on YouTube). Findings indicate that the protest movement is not only discursively constructed through the commentary that accompanies the video, but it is also constituted by non-human actants such as space, buildings and clothing. The protest movement mobilizes networks of human and non-human actants that invoke a moral authority that resists legally authorized state-sponsored networks which are also made up of human and non-human actants.
The Benefit of Insulin Degludec/Liraglutide (IDegLira) Compared With Basal-Bolus Insulin Therapy is Consistent Across Participant Subgroups With Type 2 Diabetes in the DUAL VII Randomized Trial
Authors: Billings Liana K. , Agner Bue F. Ross , Altuntas Yuksel , Grøn Randi , Halladin Natalie , Klonoff David C. , Tentolouris Nikolaos , Jódar Esteban
Published: February 2020

Background:

Insulin degludec/liraglutide (IDegLira) results in glycated hemoglobin (HbA1c) levels comparable with basal-bolus (BB) therapy. Here, we assessed the effect of once-daily IDegLira compared with BB (once-daily insulin glargine 100 U/mL and insulin aspart ≤4 times/day) across subgroups with varying characteristics.

Materials and Methods:

DUAL VII trial participants (type 2 diabetes [T2D], HbA1c 53-86 mmol/mol [7.0%-10.0%]) were subgrouped post hoc based on the following baseline characteristics: HbA1c (≤58.5, >58.5 to ≤69.4, and >69.4 mmol/mol; ≤7.5%, >7.5 to ≤8.5%, and >8.5%), body mass index (<30, ≥30 to <35, and ≥35 kg/m2), age (18 to <65 and ≥65 years), duration of diabetes (≥0 to 10 and ≥10 years), total pretrial daily basal insulin dose (20 to <30, ≥30 to <40, and ≥40 to ≤50 U), and fasting plasma glucose (<7.2 mmol/L/<130 mg/dL and ≥7.2 mmol/L/≥130 mg/dL).

Results:

Compared with BB, and in all subgroups, IDegLira treatment consistently gave similar HbA1c reductions, less severe or blood glucose-confirmed hypoglycemia, lower end-of-trial (EOT) total daily insulin dose, and weight loss. In all subgroups, mean EOT HbA1c was ≤53 mmol/mol (≤7.0%). The greatest HbA1c reduction occurred in the highest baseline HbA1c subgroup. Overall, mean EOT daily insulin dose was 0.43 to 0.52 U/kg with IDegLira and 0.74 to 1.07 U/kg with BB. More participants achieved the triple composite endpoint (HbA1c <53 mmol/mol [<7.0%] without weight gain or hypoglycemia) with IDegLira vs BB across the baseline HbA1c subgroups (≤58.5 mmol/mol [44.6% vs 7.0%], >58.5 to ≤69.4 mmol/mol [41.1% vs 8.3%], and >69.4 mmol/mol [23.8% vs 3.4%]).

Conclusion:

These results support initiating IDegLira in patients with varying baseline characteristics and uncontrolled T2D on basal insulin.

ClinicalTrials.gov registration:

NCT02420262
Allyship and solidarity, not therapy, in child and youth mental health: Lessons from a participatory action research project with psychiatrized youth
Authors: Liegghio Maria
Published: February 2020

While globally advances have been made to recognize children as social actors in their own right, for psychiatrized young people their experiences of distress are often seen as a limitation and thus used as a justification for denying their meaningful participation in matters of concern to their lives. However, what would it mean if ‘mental illness’ was not seen as a ‘limitation’, but rather as an ‘epistemological position’ from which the social world is experienced, understood and acted upon? What would it mean if our theories about ‘distress’ and ‘helping’ were premised on the subjugated knowledges of psychiatrized children and youth? The consumer/survivor-led research movement has made significant gains in answering these questions for the adult, but not necessarily for the child and youth mental health field. The purpose of this article is to critically examine the significance of psychiatrized young people setting and executing their own research and, ultimately, practice agendas. Presented are the outcomes of an evaluation of a participatory action research project examining the stigma of mental illness conducted with seven psychiatrized youth, 14 to 17 years old. The outcomes suggest our roles as practitioners and researchers need to shift from being ‘agents’ working on behalf of to ‘allies’ working in solidarity with young people to change the social conditions of their marginalization. The article concludes with the limits of consumer/survivor-led research for addressing adultism and, instead, ends with a call for decolonizing children’s mental health.
‘Looping effects’ related to young people’s mental health: How young people transform the meaning of psychiatric concepts
Authors: Lindholm Sofia Kvist , Wickström Anette
Published: February 2020

Over the past decades, reports from official authorities and the media have suggested that there is a ‘crisis’ in young people’s mental health. However, there is considerable uncertainty regarding how to interpret the data referred to in these alarming reports. The present article draws on ‘the minority voices’ of young people and theories developed by Ian Hacking to undertake a critical analysis of the conceptualisation of young people’s mental health. According to Hacking, systems of classifications formulate general truths about people and frame the suffering of individuals in specific ways. Classification changes people. However, young people are social actors who interact with classifications of their mental health and by doing so they could cause classifications to be redrawn. Hacking refers to these feedback effects as ‘looping effects’. Based on 51 interviews with 15-year-olds, this article explores how young people interact with psychiatric labels associated with their wellbeing such as anxiety and depression. We demonstrate how the participants gave new meaning to these psychiatric labels, devalued and gave nuance to them, and by doing so transformed them into cultural categories rather than diagnostic categories. We discuss the potential looping effects related to young people’s mental health and how the present findings can inform policy practice.
Troubling the ‘troubled teen’ industry: Adult reflections on youth experiences of therapeutic boarding schools
Authors: Golightley Sarah
Published: February 2020

In the United States, thousands of young people reside in private schools aimed at reforming ‘troubled teens’. These ‘troubled teens’ are young people who are considered to have emotional, behavioural and/or substance misuse problems. Therapeutic boarding schools are programmes that combine educational classes and group therapy in a self-contained residential facility that runs year-round. Case study interviews with former US-based therapeutic boarding school students demonstrate the role of sanism, adultism and epistemic injustice in constructing and regulating the ‘troubled teen’. The schools’ strict structure and surveillance culture could not override students will and their ability to find means to resist. The article’s central aim is to centre the perspectives of former students and critique social control of young people in therapeutic boarding schools.
Prune-belly syndrome in Africa: An analysis and systematic review of cases, etiology, treatment, and outcomes
Authors: Keet Kerri , Henry Brandon Michael , Tubbs R Shane
Published: February 2020

Background:

Prune-belly syndrome is a rare congenital disorder characterized by a spectrum of three anomalies: bilateral undescended testes, dilated urinary tract, and anterior abdominal muscle deficiency.

Objectives:

In developing countries, inadequate access to health care may affect treatment and outcomes of prune-belly syndrome. This study’s goal was to review the anatomical features, etiology, genetics, management, and outcomes of cases in Africa.

Methods:

PubMed was searched to identify case reports and case studies describing prune-belly syndrome in Africa. Data collected from each study included the number of cases, age at diagnosis, sex, description of the abdominal muscles, testes, and urinary tract, as well as associated anomalies, management, and long-term outcomes.

Results:

A total of 16 publications that reported 58 cases in African countries were included. The prevalence of female patients (15.5%) was higher than in developed countries (3%). The abdominal muscles were deficient in all cases, and bilateral cryptorchidism was present in nearly all males (96%). Distension of the bladder was common, with normal anatomy reported in only one case. Bilateral hydroureters and hydronephrosis also were present in the majority of cases. Only six cases (10.3%) had no associated anomalies, such as musculoskeletal or cardiovascular. Karyotyping was performed in only three cases (5.2%) because of limited hospital facilities. Six parents (10.3%) declined treatment for their children, 12 cases (20.7%) were managed conservatively, and 25 (43.1%) received surgical intervention. Patients’ mortality rate was higher than in developed countries.

Conclusion:

Diagnosis and treatment of prune-belly syndrome remains a challenge in Africa, in which multiple factors, such as access to health care and cultural beliefs, affect mortality rates and outcomes. Patient education and support groups may improve compliance with treatment.

Level of evidence:

Not applicable for this multicenter audit.
Nasal adenocarcinoma as a suspected secondary malignant neoplasm in a cat previously treated for nasal lymphoma
Authors: Callanan Gabrielle F , Curran Kaitlin M , Parachini-Winter Cyril , Bracha Shay , Spagnoli Sean , Russell Duncan S , Stieger-Vanegas Susanne M , Griffin Lynn , Leeper Haley
Published: February 2020

Case summary

A case of nasal adenocarcinoma as a suspected secondary malignant neoplasm following definitive radiation therapy and multiagent chemotherapy for nasal lymphoma is described. An 11-year-old spayed female domestic shorthair cat was presented for a 3-week history of progressive facial swelling located over the nasal planum and extending to the medial canthus of the right eye. The cat was previously diagnosed with nasal lymphoma and treated with chemotherapy and definitive radiation 2.5 years prior. Although a definitive diagnosis could not be obtained via cytology, recurrent lymphoma was suspected based on the cat’s history and recurrent clinical signs. A lymphoma-directed chemotherapy protocol was attempted, but no clinical response was achieved. The cat was euthanased owing to progressive clinical signs and a diagnosis of nasal adenocarcinoma was made on necropsy examination. Both the original diagnosis of nasal lymphoma and the secondary diagnosis of nasal adenocarcinoma were confirmed with immunohistochemistry.

Relevance and novel information

Secondary malignant neoplasm following radiation therapy is infrequently reported in the veterinary literature. In the few reports that exist, most have described sarcoma development in the dog following radiation therapy. In the present report, we describe a cat with a suspected radiation-induced nasal adenocarcinoma that developed 2.5 years after definitive radiation treatment for nasal lymphoma.
Risk Factors and Complications Following Arthroscopic Tenotomy of the Long Head of the Biceps Tendon
Authors: Mirzayan Raffy , McCrum Christopher , Butler Rebecca K. , Alluri Ram Kiran
Published: February 2020

Background:

Controversy exits regarding performing a tenotomy versus a tenodesis of the long head of the biceps tendon (LHBT).

Purpose:

To evaluate the complications after arthroscopic tenotomy of the LHBT and characterize the incidence of cosmetic deformity, cramping, subjective weakness, and continued anterior shoulder pain (ASP). Additionally, to identify patient-related factors that may predispose a patient to these complications.

Study Design:

Case-control study; Level of evidence, 3.

Methods:

Records of patients who underwent an arthroscopic LHBT tenotomy at an integrated health care system under the care of 55 surgeons were retrospectively reviewed. Exclusion criteria included LHBT tenodesis, arthroplasty, neoplastic, or fracture surgery; age younger than 18 years; incomplete documentation of physical examination; or incomplete operative reports. Characteristic data, concomitant procedures, LHBT morphology, and postoperative complications were recorded. Patients with and without postoperative complications—including cosmetic deformity, subjective weakness, continued ASP, and cramping—were analyzed by age, sex, dominant arm, body mass index (BMI), smoking status, workers’ compensation status, and intraoperative LHBT morphology to identify risk factors for developing these postoperative complications.

Results:

A total of 192 patients who underwent LHBT tenotomy were included in the final analysis. Tenotomy was performed with concomitant shoulder procedures in all but 1 individual. The mean ± SD patient age was 60.6 ± 9.5 years, and 55% were male. The overall complication rate was 37%. The most common postoperative complications include cosmetic (Popeye) deformity (14.1%), subjective weakness (10.4%), cramping (10.4%), and continued postoperative ASP over the bicipital groove (7.8%). Every 10-year increase in age was associated with 0.52 (95% CI, 0.28-0.94) times the odds of continued ASP and 0.59 (95% CI, 0.36-0.98) times the odds of cramping pain. Male patients had 3.9 (95% CI, 1.4-10.8) times the odds of cosmetic (Popeye) deformity. Patients who had active workers’ compensation claims had 12.5 (95% CI, 2.4-63.4) times the odds of having continued postoperative ASP. Tenotomy on the dominant arm, BMI, and active smoking status demonstrated no statistically significant association with postoperative complications.

Conclusion:

Patients experiencing complications after tenotomy were significantly younger and more likely to be male and to have a workers’ compensation injury. LHBT tenotomy may best be indicated for elderly patients, female patients, and those without active workers’ compensation claims.
Results and Recommendations From a National Public Health Workforce Development Systems Assessment Conducted in the United States
Authors: Welter Christina R. , Bekemeier Betty , McKeever Jennifer
Published: February 2020

Multiple public health workforce development assessments report individual worker knowledge and skill-based training needs. These assessments do not capture practitioners’ in-depth perceptions of complex public health challenges and whether workforce development approaches address these issues. To address this gap, the Public Health Learning Network—a national coalition of 10 Regional Public Health Training Centers located at United States accredited schools of public health, their partners, and the National Network of Public Health Institutes—conducted a public health workforce development assessment using a two-phased mixed-method design to explore systems-level gaps and opportunities for improving workforce development effectiveness. Phase 1 included a content analysis of major public health workforce development reports and peer-reviewed literature. Phase 2 included primary qualitative data collection of key informant interviews and focus groups via conference call with 43 participants representing 41 public health organizations at the local, state, and national levels. Results included a wide range of challenges with an emphasis on major systems changes, the shift in public health’s role to more effectively build community collective capacity, limited staff capacity and capability, and the need for more flexible and integrated training funding. Public health workforce development approaches recommended to address these challenges included improving pedagogical approaches toward more integrated, multimodal training delivered over time; increasing workforce capacity to address complex challenges such as racism and housing; and facilitating public health workforce development system coordination and alignment. Public Health Learning Network’s workforce assessment also identified opportunities for conceptualizing the definition and delivery of training toward ongoing learning.
Insider status: (Re)framing researcher positionality in international human resource management studies
Authors: Collins Heidi , McNulty Yvonne
Published: February 2020

Insider researchers in international human resource management study not only the organisations or communities they may be members of, but also the people they perceive closeness with or with whom they share identities. The research context for insiders is rich but rarely problem-free. The uncertainty and dynamism of insider research often leaves insider researchers grappling with methodological and ethical challenges, but with no explicit framework to guide how they can be addressed in research practice or research reporting. Indeed, the ‘sanitised’ methodologies appearing in published work often do not reflect the complexity of the authors’ experience. In this article, we call for researchers to give more explicit consideration of what it means to research from an insider position, regardless of the research paradigm from which they work. The article addresses some of the messy details of insider research, drawing on personal accounts of our own research practice. We offer a framework of researcher personae as a tool for reflecting upon researchers’ insider positionality before, during and after data have been collected and analysed. Overall, we encourage researchers working from insider positions to adopt three practices: (1) to engage in reflexive consideration of the effects of (changing) positionality on their work as a way to strengthen the ethical and theoretical outcomes of research practice; (2) to acknowledge and leverage, rather than conceal, insiderness as a key strategy for making feasible more research on sensitive and ‘taboo’ topics; and (3) to make positionality explicit in research reporting in order to enhance the quality of insider research as well as enhance fieldwork learning more generally in the international human resource management field. While the personal accounts we share in this article are based on our research within the field of international human resource management, the intention is that it may also be of value to researchers in other fields.
Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts
Authors: Hyder Iqbal , Eghbalsaied Shahin , Kues Wilfried A.
Published: February 2020

Background

Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited proliferative capacity. Improving the gene transfer by using square wave electroporation in difficult to transfect cells, like bovine fetal fibroblasts, is a prerequisite for transgenic and further downstream experiments.

Results

Here, bovine fetal fibroblasts were used for square-wave electroporation experiments in which the following parameters were systematically tested: electroporation buffer, electroporation temperature, pulse voltage, pulse duration, pulse number, cuvette type and plasmid DNA amount. For the experiments a commercially available square-wave generator was applied. Post electroporation, the bovine fetal fibroblasts were observed after 24 h for viability and reporter expression. The best results were obtained with a single 10 millisecond square-wave pulse of 400 V using 10 μg supercoiled plasmid DNA and 0.3 × 106 cells in 100 μl of Opti-MEM medium in 4 mm cuvettes. Importantly, the electroporation at room temperature was considerably better than with pre-cooled conditions.

Conclusions

The optimized electroporation conditions will be relevant for gene transfer experiments in bovine fetal fibroblasts to obtain genetically engineered donor cells for somatic cell nuclear transfer and for reprogramming experiments in this species.
Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice
Authors: Cui Yue , Jiang Nan , Xu Zhengjin , Xu Quan
Published: February 2020

Background

The heterotrimeric G protein complex, consisting of Gα, Gβ, and Gγ subunits, are conserved signal transduction mechanism in eukaryotes. Recent molecular researches had demonstrated that G protein signaling participates in the regulation of yield related traits. However, the effects of G protein genes on yield components and stress tolerance are not well characterized.

Results

In this study, we generated heterotrimeric G protein mutants in rice using CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) gene-editing technology. The effects of heterotrimeric G proteins on the regulation of yield components and stress tolerance were investigated. The mutants of gs3 and dep1 generated preferable agronomic traits compared to the wild-type, whereas the mutants of rga1 showed an extreme dwarf phenotype, which led to a dramatic decrease in grain production. The mutants showed improved stress tolerance, especially under salinity treatment. We found four putative extra-large G proteins (PXLG)1–4 that also participate in the regulation of yield components and stress tolerance. A yeast two hybrid showed that the RGB1 might interact with PXLG2 but not with PXLG1, PXLG3 or PXLG4.

Conclusion

These findings will not only improve our understanding of the repertoire of heterotrimeric G proteins in rice but also contribute to the application of heterotrimeric G proteins in rice breeding.
Knowledge and attitudes towards E-cigarette use in Lebanon and their associated factors
Authors: Aghar Hanan , El-Khoury Nathalie , Reda Mahasen , Hamadeh Wissam , Krayem Hussein , Mansour Mohammad , Raouf Hawraa , Jaffa Miran A.
Published: February 2020

Background

Despite the misconceptions regarding E-cigarettes (ECs), only a few studies have been conducted in the Middle East that focused on this topic. This study assesses the knowledge of and attitudes towards ECs in Lebanon, determines how these two measures are associated, and identifies the variables that explain each of these measures.

Methods

A cross sectional study was conducted on a convenience sample of Lebanese pedestrians aged between 18 and 64 inclusive. A structured self-administered questionnaire comprising of knowledge and attitude scales, and questions on demographical, health and smoking characteristics was used.

Results

Scores for attitudes and knowledge of ECs were summed and dichotomized using a 75% cutoff, above which the participant was considered to have a positive attitude and good knowledge. Among the 352 participants (56.6% males, 43.3% females, mean age 30.3, 46.2% smokers), 63.3% exhibited a lower level of EC knowledge. More than 50% erroneously thought that ECs are not associated with lung and bladder cancer or impair lung and heart function. 65% falsely thought that it is harmless and not addictive. As for attitude, 43.3, 53.9, and 44.3% thought that it is socially acceptable, helps in smoking cessation, and is a good replacement for cigarettes and an enjoyable recreational device respectively. Our results revealed an inverse correlation between attitude and knowledge scores (Spearman’s correlation = −.30, p < .001). Predictors of knowledge included health-related occupation (p = .010), regular exercise (p = .016), healthy diet (p = .026), EC use (p = .026), perception that ECs are not harmful (p = .001), and help in smoking cessation (p = .017). Predictors of attitude included EC use (p = .008), sex (p = .010), and knowledge that most ECs are addictive (p = .006), harmful (p = .014), and impair heart and lung function (p = .047).

Conclusions

Our study revealed a gap in EC knowledge, especially among participants who displayed a positive attitude towards ECs. Hence, measures should be undertaken to regulate its use by instituting more stringent laws and holding nationwide awareness campaigns.
Occipitocervical fusion complicated with cerebellar abscess: a case report
Authors: Lee Cheng-Chi , Liu Yu-Tse
Published: February 2020

Background

Occipitocervical (OC) fusion is indicated for OC instability and other conditions. Surgical complications include infection, malunion, and instrument failure.

Case presentation

We described a patient who underwent OC fusion and subsequently developed complication of cerebellar abscess and obstructive hydrocephalus. A 63-year-old male patient had been suffering from long-term neck pain and limb numbness and weakness. Cervical spine examination revealed tight stenosis at C1 level and instability in the C1-C2 joints. A C1 laminectomy with OC fusion was performed, and the patient was discharged. Unfortunately, a few days later, he went to the emergency department and complained of persistent dizziness, vomiting, and unsteady gait. Computed tomography (CT) and magnetic resonance imaging (MRI) images revealed a suspicious cerebellar abscess formation and hydrocephalus. Furthermore, CT images indicated that the left screw was loose, and the diameter of the right screw hole was much larger than the size of the screw. Besides, inappropriate length of the screw penetrated the occipital bone and may cause the disruption of dura mater. The patient underwent external ventricular drainage first, followed by abscess drainage and C1-C2 fixation a few days later. He was discharged without any further neurological deficits or infectious problems. The patient recovered with intact consciousness, full muscle strength, and improved numbness throughout the extremities, with a Nurick grade of 1. A follow-up magnetic resonance imaging at 3 months after surgery revealed near total resolution of the abscess. Inform consent was obtained from this patient.

Conclusions

Carefully conducting the procedure using the most tailored approach is essential to successful surgery, but this rare complication should always be kept in mind.
The emerging roles of N6-methyladenosine (m6A) deregulation in liver carcinogenesis
Authors: Chen Mengnuo , Wong Chun-Ming
Published: February 2020

Liver cancer is a common cancer worldwide. Although the etiological factors of liver carcinogenesis are well defined, the underlying molecular mechanisms remain largely elusive. Epigenetic deregulations, such as aberrant DNA methylation and histone modifications, play a critical role in liver carcinogenesis. Analogous to DNA and core histone proteins, reversible chemical modifications on mRNA have recently been recognized as important regulatory mechanisms to control gene expression. N6-methyladenosine (m6A) is the most prevalent internal mRNA modification in mammalian cells. m6A modification is important for controlling many cellular and biological processes. Deregulation of m6A modification has been recently implicated in human carcinogenesis, including liver cancer. In this review, we summarize the recent findings on m6A regulation and its biological impacts in normal and cancer cells. We will focus on the deregulation of m6A modification and m6A regulators in liver diseases and liver cancers. We will highlight the clinical relevance of m6A deregulation in liver cancer. We will also discuss the potential of exploiting m6A modification for cancer diagnosis and therapeutics.