bluuebunny/crossref_metadata_2025 huggingface dataset:
Introducing Artificial Intelligence in Interpretation of Foetal Cardiotocography: Medical Dataset Curation and Preliminary Coding—An Interdisciplinary Project
Authors: Aeberhard Jasmin Leonie , Radan Anda-Petronela , Soltani Ramin Abolfazl , Strahm Karin Maya , Schneider Sophie , Carrié Adriana , Lemay Mathieu , Krauss Jens , Delgado-Gonzalo Ricard , Surbek Daniel
Published: January 2024

Artificial intelligence (AI) is gaining increasing interest in the field of medicine because of its capacity to process big data and pattern recognition. Cardiotocography (CTG) is widely used for the assessment of foetal well-being and uterine contractions during pregnancy and labour. It is characterised by inter- and intraobserver variability in interpretation, which depends on the observers’ experience. Artificial intelligence (AI)-assisted interpretation could improve its quality and, thus, intrapartal care. Cardiotocography (CTG) raw signals from labouring women were extracted from the database at the University Hospital of Bern between 2006 and 2019. Later, they were matched with the corresponding foetal outcomes, namely arterial umbilical cord pH and 5-min APGAR score. Excluded were deliveries where data were incomplete, as well as multiple births. Clinical data were grouped regarding foetal pH and APGAR score at 5 min after delivery. Physiological foetal pH was defined as 7.15 and above, and a 5-min APGAR score was considered physiologic when reaching ≥7. With these groups, the algorithm was trained to predict foetal hypoxia. Raw data from 19,399 CTG recordings could be exported. This was accomplished by manually searching the patient’s identification numbers (PIDs) and extracting the corresponding raw data from each episode. For some patients, only one episode per pregnancy could be found, whereas for others, up to ten episodes were available. Initially, 3400 corresponding clinical outcomes were found for the 19,399 CTGs (17.52%). Due to the small size, this dataset was rejected, and a new search strategy was elaborated. After further matching and curation, 6141 (31.65%) paired data samples could be extracted (cardiotocography raw data and corresponding maternal and foetal outcomes). Of these, half will be used to train artificial intelligence (AI) algorithms, whereas the other half will be used for analysis of efficacy. Complete data could only be found for one-third of the available population. Yet, to our knowledge, this is the most exhaustive and second-largest cardiotocography database worldwide, which can be used for computer analysis and programming. A further enrichment of the database is planned.
Phogrin Regulates High-Fat Diet-Induced Compensatory Pancreatic β-Cell Growth by Switching Binding Partners
Authors: Kubota Chisato , Torii Ryoko , Hosaka Masahiro , Takeuchi Toshiyuki , Gomi Hiroshi , Torii Seiji
Published: January 2024

The receptor protein tyrosine phosphatase phogrin primarily localizes to hormone secretory granules in neuroendocrine cells. Concurrent with glucose-stimulated insulin secretion, phogrin translocates to pancreatic β-cell plasma membranes, where it interacts with insulin receptors (IRs) to stabilize insulin receptor substrate 2 (IRS2) that, in turn, contributes to glucose-responsive β-cell growth. Pancreatic β-cell development was not altered in β-cell-specific, phogrin-deficient mice, but the thymidine incorporation rate decreased in phogrin-deficient islets with a moderate reduction in IRS2 protein expression. In this study, we analyzed the β-cell response to high-fat diet stress and found that the compensatory expansion in β-cell mass was significantly suppressed in phogrin-deficient mice. Phogrin–IR interactions occurred only in high-fat diet murine islets and proliferating β-cell lines, whereas they were inhibited by the intercellular binding of surface phogrin under confluent cell culture conditions. Thus, phogrin could regulate glucose-stimulated compensatory β-cell growth by changing its binding partner from another β-cell phogrin to IR in the same β-cells.
Lifestyle Interventions in an Aged Population: Challenges and Opportunities from a Public Health Perspective
Authors: Cano-Ibáñez Naomi , Bueno-Cavanillas Aurora
Published: January 2024

In recent decades, the prevalence of non-communicable diseases (NCDs) such as obesity, type 2 diabetes mellitus, cancer and cardiovascular disease has increased worldwide [...]
Preparing Collared Peccary (Pecari tajacu Linnaeus, 1758) for Reintroduction into the Wild: A Screening for Parasites and Hemopathogens of a Captive Population
Authors: Silveira Júlia Angélica Gonçalves da , Moreira Simone Magela , Nascimento Ariane Flávia do , Oliveira Marco Miguel de , Santos Hudson Andrade dos , Estevam Letícia Gracielle Tôrres de Miranda , Pereira Carine Rodrigues , Oliveira Anna Gabriela Guimarães , D’Elia Mirella Lauria , Araujo Andreina de Carvalho , Silva Juliana Macedo Magnino
Published: January 2024

The reintroduction of captive animals to the wild helps restore endangered species, but it risks pathogen transmission, harming wild populations. Such transmission can impact the genetic diversity and long-term viability of these populations. This study assessed parasite diversity and load in captive Pecari tajacu, a species native to the Americas and culturally significant to Brazilian indigenous culture, prior to reintroduction. Samples from 24 peccaries were analyzed for ectoparasites, hemopathogens, and stool parasites with direct and molecular analysis. Findings showed that various parasites were present. Two peccaries (8.3%) were infested by the adult tick Amblyomma sculptum. Six (25.0%) tested positive for Trypanosoma evansi, four (16.7%) for hemobacteria of the family Anaplasmataceae, twelve (50.0%) for hemotropic Mycoplasma, and seven (29.2%) for Leishmania braziliensis. Stool samples indicated multiple parasites, with sixteen (66.7%) peccaries infected by Strongylida order parasites, Spiruridae in three (12.5%), and Ascaris suum in one (4.2%) animal. Cysts of Balantidium sp. were found in twenty (83.3%), Entamoeba polecki in five (20.8%), and Iodamoeba bütschlii in two (8.3%) peccaries. To our current knowledge, this is the first global report of Leishmania braziliensis, Iodamoeba bütschlii, and Entamoeba polecki in P. tajacu, irrespective of the environment, including both captivity and wild conditions. Some of these parasites are common in domestic animals, and others are zoonotic, indicating potential interspecies pathogen transmission.
The Efficacy and Safety of Polyethylene Glycol Cholesterol- and Tocopherol Polyethylene Glycol 1000 Succinate-Modified Transforming Growth Factor β1 Small Interfering RNA Lipid Nanoparticles in the Treatment of Paclitaxel-Resistant Non-Small-Cell Lung Cancer
Authors: Zeng Zhaowu , Zeng Xianglong , Li Xinyi , Feng Yuxin , Kan Yue , Liu Xingyan , Zeng Yiying
Published: January 2024

The aim of this study was to explore the efficacy and safety of TGFβ1 siRNA lipid nanoparticles (LNPs) modified with different PEG derivatives (PEG5000 cholesterol, abbreviated as CE; tocopherol polyethylene glycol 1000 succinate, abbreviated as TPGS) in the treatment of paclitaxel-resistant non-small-cell lung cancer. Three kinds of TGFβ1 siRNA LNPs were prepared via microfluidics technology, using different PEG derivatives and dosages (CE1.5, CE2.5, TPGS2.5) as variables. Their particle size, zeta potential, contents, and encapsulation efficiencies were determined. The inhibition of TGFβ1 mRNA and protein expression and the effects of the three kinds of LNPs on the proliferation of paclitaxel-resistant non-small-cell lung cancer cells (A549/T cell) were characterized. The distributions of the three siRNA LNPs in nude mice bearing A549/T tumors, especially at the tumor site, were observed using in vivo mouse imaging technology, and their corresponding efficacies were evaluated. The average particle size of the three kinds of TGFβ1 siRNA LNPs was about 70–80 nm, and they were capable of charge flipping. All three siRNA LNPs could effectively inhibit the expression of TGFβ1 mRNA and protein in A549/T cells and inhibit the proliferation of A549/T cells in vitro. The results of in vivo mice imaging showed that the three kinds of siRNA LNPs, when labeled with cypate, retain strong fluorescence in the tumor at 24 h. The pharmacodynamic results, such as for relative tumor volumes and tumor inhibition rates, reveal that TGFβ1 siRNA LNPs modified with CE1.5, CE2.5, or TPGS2.5 can be used to effectively treat paclitaxel-resistant lung adenocarcinoma. The histopathological results showed that the three kinds of LNPs have a certain toxicity but are relatively safe compared to common forms of chemotherapy such as cabazitaxel. TGFβ1 siRNA LNPs modified with CE1.5, CE2.5, and TPGS2.5 can inhibit TGFβ1 mRNA and protein expression in A549/T cells in vitro and can accumulate and play a role in the tumor tissue of nude mice, features that can be exploited for treating paclitaxel-resistant lung adenocarcinoma.
Total Soil CO2 Efflux from Drained Terric Histosols
Authors: Vigricas Egidijus , Čiuldienė Dovilė , Armolaitis Kęstutis , Valujeva Kristine , Laiho Raija , Jauhiainen Jyrki , Schindler Thomas , Bārdule Arta , Lazdiņš Andis , Butlers Aldis , Kazanavičiūtė Vaiva , Belova Olgirda , Kamil-Sardar Muhammad , Soosaar Kaido
Published: January 2024

Histosols cover about 8–10% of Lithuania’s territory and most of this area is covered with nutrient-rich organic soils (Terric Histosols). Greenhouse gas (GHG) emissions from drained Histosols contribute more than 25% of emissions from the Land Use, Land Use Change and Forestry (LULUCF) sector. In this study, as the first step of examining the carbon dioxide (CO2) fluxes in these soils, total soil CO2 efflux and several environmental parameters (temperature of air and topsoil, soil chemical composition, soil moisture, and water table level) were measured in drained Terric Histosols under three native forest stands and perennial grasslands in the growing seasons of 2020 and 2021. The drained nutrient-rich organic soils differed in terms of concentrations of soil organic carbon and total nitrogen, as well as soil organic carbon and total nitrogen ratio. The highest rate of total soil CO2 efflux was found in the summer months. Overall, the rate was statistically significant and strongly correlated only with soil and air temperature. A trend emerged that total soil CO2 efflux was 30% higher in perennial grassland than in forested land. Additional work is still needed to estimate the net CO2 balance of these soils.
Irrigation and Fertilization Scheduling for Peanut Cultivation under Mulched Drip Irrigation in a Desert–Oasis Area
Authors: Dong Jianshu , Shen Xiaojun , Li Qiang , Xue Zhu , Hou Xianfei , Miao Haocui , Ning Huifeng
Published: January 2024

The aim of this study was to investigate the impact of water and nitrogen regulation on the characteristics of water and fertilizer demands and the yield, quality, and efficiencies of the water and nitrogen utilization of peanuts cultivated under mulched drip irrigation in a desert–oasis region. The experiment, conducted in Urumqi, Xinjiang, centered on elucidating the response mechanisms governing peanut growth, yield, quality, water consumption patterns, and fertilizer characteristics during the reproductive period under the influence of water and nitrogen regulation. In the field experiments, three irrigation levels were implemented, denoted as W1 (irrigation water quota of 22.5 mm), W2 (irrigation water quota of 30 mm), and W3 (irrigation water quota of 37.5 mm). Additionally, two nitrogen application levels, labeled N1 (nitrogen application rate of 77.5 kg·ha−1) and N2 (a nitrogen application rate of 110 kg·ha−1), were applied, resulting in seven treatments. A control treatment (CK), which involved no nitrogen application, was also included in the experimental design. The results indicate a direct correlation between the increment in the irrigation quota and increases in farmland water-related parameters, including water consumption, daily water consumption intensity, and water consumption percentage. The nitrogen harvest index (NHI) demonstrated a higher value in the absence of nitrogen application compared to the treatment with elevated nitrogen levels. The application of nitrogen resulted in an elevation in both nitrogen accumulation and nitrogen absorption efficiency within pods and plants. When subjected to identical nitrogen application conditions, irrigation proved to be advantageous in enhancing water-use efficiency (WUE), nitrogen partial factor productivity (NPFP), and the yield of peanut pods. The contribution rate of water to pod yield and WUE exceeded that of nitrogen, while the contribution rate of nitrogen to nitrogen-use efficiency (NUE) was higher. The total water consumption for achieving a high yield and enhanced water- and nitrogen-use efficiencies in peanuts cultivated under drip irrigation with film mulching was approximately 402.57 mm. Taking into account yield, quality, and water- and nitrogen-used efficiencies, the use of an irrigation quota of 37.5 mm, an irrigation cycle of 10–15 days, and a nitrogen application rate of 110 kg·ha−1 can be regarded as an appropriate water and nitrogen management approach for peanut cultivation under mulched drip irrigation in Xinjiang.
Optimization of Low-Tech Protected Structure and Irrigation Regime for Cucumber Production under Hot Arid Regions of India
Authors: Kumar Pradeep , Khapte Pratapsingh S. , Singh Akath , Saxena Anurag
Published: January 2024

Water scarcity and climate variability impede the realization of satisfactory vegetable yields in arid regions. It is imperative to delve into high-productivity and water-use-efficient protected cultivation systems for the sustained supply of vegetables in harsh arid climates. A strenuous effort was made to find suitable protected structures and levels of irrigation for greenhouse cucumber production in hot arid zones of India. In this endeavor, the effects of three low-tech passively ventilated protected structures, i.e., naturally ventilated polyhouse (NVP), insect-proof screenhouse (IPS) and shade screenhouse (SHS), as well as three levels of irrigation (100%, 80% and 60% of evapotranspiration, ET) were assessed for different morpho-physiological, yield and quality traits of the cucumber in a two-year study. Among the low-tech protected structures, NVP was found superior to IPS and SHS for cucumber performance, as evidenced by distinctly higher fruit yields (i.e., 31% and 121%, respectively) arising as a result of higher fruit number/plants and mean fruit weights under NVP. The fruit yield decreased in response to the degree of water shortage in deficit irrigation across all protected structures. However, the interaction effect of the protected structure and irrigation regime reveals that plants grown under moderate deficit (MD, 20% deficit) inside NVP could provide higher yields than those obtained under well-watered (WW, 100% of ET) conditions inside IPS or SHS. Plant growth indices such as vine length, node number/plant, and shoot dry mass were also measured higher under NVP. The greater performance of cucumber under NVP was attributed to a better plant physiological status (i.e., higher photosystem II efficiency, leaf relative water content and lower leaf water potential). The water deficit increased water productivity progressively with its severity; it remained higher in NVP, as reflected by 20% and 94% higher water productivity than those recorded in IPS and SHS, respectively, across different irrigation levels. With the exception of total soluble solids and fruit dry matter content (which were recorded higher), fruit quality parameters were reduced under water deficit conditions. The findings of this study emphasize the importance of considering suitable low-tech protected structures (i.e., NVP) and irrigation levels (i.e., normal rates for higher yields and moderate deficit (−20%) for satisfactory yields) for cucumber in hot arid regions. The results provide valuable insights for growers as well as researchers aiming to increase vegetable production under harsh climates and the water-limiting conditions of arid regions.
Preparation and Performance of Bitumen Modified by Melt-Blown Fabric of Waste Mask Based on Grey Relational and Radar Chart Analysis
Authors: Cheng Peifeng , Zheng Chunmeng , Zhang Zhanming , Li Yiming , Huang Kai , Yu Dezhong , Ji Yongcheng
Published: January 2024

To effectively utilize waste mask materials in road engineering and minimize resource waste, the melt-blown fabric (MBF) of waste masks was utilized to modify the virgin bitumen. The preparation process of MBF-modified bitumen was investigated, and the physical and rheological properties of bitumen were measured. Subsequently, the blending mechanism during preparation and the dispersion morphology of the modifier were explored. Finally, the pavement performance of the mixture was investigated, and a radar chart analysis was performed to quantitatively assess the effects of MBF modification. Results suggested that the recommended preparation process of shear time, shear rate, and shear temperature was 170 °C, 4000 r/min, and 15 min, respectively. MBF enhanced the high-temperature stability of the binder and weakened the temperature susceptibility. The modification was primarily a physical process. No network structure and agglomeration formed in the bitumen after modification. The addition of MBF significantly improved the resistance of the asphalt mixture to a high-temperature deformation and water damage but harmed its low-temperature crack resistance. The comprehensive assessment results of 0% (f1), 1% (f2), 3% (f3), and 5% (f4) MBF to improve the properties of the mixture were in the following order: f3>f4>f2>f1, where the impact of 3% MBF was the most significant, followed by 5% and 1% MBF.
Influence of Electrospinning Parameters on the Morphology of Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Fibrous Membranes and Their Application as Potential Air Filtration Materials
Authors: Liu Yaohui , Wang Yanming , Lee Cheng-Hao , Kan Chi-Wai , Lu Xiaoying
Published: January 2024

A large number of non-degradable materials have severely damaged the ecological environment. Now, people are increasingly pursuing the use of environmentally friendly materials to replace traditional chemical materials. Polyhydroxyalkonates (PHAs) are receiving increasing attention because of the unique biodegradability and biocompatibility they offer. However, the applications of PHAs are still limited due to high production costs and insufficient study. This project examines the optimal electrospinning parameters for the production of PHA-based fibrous membranes for air filtration. A common biodegradable polyester, Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), was electrospun into a nanofibrous membrane with a well-controlled surface microstructure. In order to produce smooth, bead-free fibers with micron-scale diameters, the effect of the process parameters (applied electric field, solution flow rate, inner diameter of hollow needle, and polymer concentration) on the electrospun fiber microstructure was optimized. The well-defined fibrous structure was optimized at an applied electric field of 20 kV, flow rate of 0.5 mL/h, solution concentration of 12 wt.%, and needle inner diameter of 0.21 mm. The morphology of the electrospun PHBV fibrous membrane was observed by scanning electron microscopy (SEM). Fourier transform infrared (FTIR) and Raman spectroscopy were used to explore the chemical signatures and phases of the electrospun PHBV nanofiber. The ball burst strength (BBS) was measured to assess the mechanical strength of the membrane. The small pore size of the nanofiber membranes ensured they had good application prospects in the field of air filtration. The particle filtration efficiency (PFE) of the optimized electrospun PHBV fibrous membrane was above 98% at standard atmospheric pressure.
In Situ Polymerization of Antibacterial Modification Polyamide 66 with Au@Cu2O-ZnO Ternary Heterojunction
Authors: Li Xiang , Zheng Mi , Zhao Shikun , Cao Zhiwen , Pan Kai , Feng Xinxing , Zhang Hua , Zheng Min , Wang Cheng
Published: January 2024

In situ polymerization has proven to be an effective route through which to introduce function materials into polyamide materials. In this work, a nano-heterojunction material was evenly dispersed in PA66 via in situ polymerization methods to yield the antimicrobial PA66. The composites showed excellent antibacterial activity against Staphylococcus aureus and Escherichia coli, with strong mechanical properties. Fourier transform infrared spectroscopy (FTIR) showed that metal ions reacted with oxygen-containing functional groups. In addition, the shift of oxygen peaks in XPS spectra confirmed the occurrence of a complexation reaction. X-ray diffraction (XRD) and differential scanning calorimetry (DSC) confirmed the effect of nano-heterojunction, which induced crystallization. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed uniform dispersion of heterojunctions in PA66. Tensile testing revealed decreased toughness with higher loadings. The nanocomposite polyamide material has good processing properties which can be processed into thin films, molds, and wires without changing the morphology, and can be widely used in a variety of fields.
Pyrolytic Modification of Heavy Coal Tar by Multi-Polymer Blending: Preparation of Ordered Carbonaceous Mesophase
Authors: Zhang Lei , Liu Chunjiang , Jia Yang , Mu Yidan , Yan Yao , Huang Pengcheng
Published: January 2024

In order to achieve the high-value utilization of heavy tar for the production of enhanced-performance graphite foam carbon, the carbon mesophase was ready from the heavy component of low-temperature coal tar, and the coal tar was modified by styrene-butadiene-styrene (SBS), polyethylene (PE) and ethylene-vinyl-acetate (EVA) copolymers. The order degree of the carbonite mesophase was analyzed using a polarizing microscope test, Fourier transform infrared spectroscopy and X-ray diffraction to screen out the most suitable copolymer type and addition amount. Furthermore, the mechanism of modification by this copolymer was analyzed. The results showed that adding SBS, PE and EVA to coal tar would affect the order of carbonaceous mesophase; however, at an addition rate of 10.0 wt.%, the linear-structure SBS copolymer with a styrene/butadiene ratio (S/B) of 30/70 exhibited the optimal degree of ordering in the carbonaceous mesophase. Its foam carbon prepared by polymer modification is the only one that forms a graphitized structure, with d002 of 0.3430 nm, and the maximum values of Lc and La are 3.54 nm and 2.22 nm, respectively. This is because, under elevated pressure and high-temperature conditions, SBS underwent chain scission, releasing a more significant number of methyl and other free radicals that interacted with the coal tar constituents. As a result, it reduced the affinity density of heavy coal tar molecules, enhanced fluidity, promoted the stacking of condensed aromatic hydrocarbons and increased the content of soluble carbonaceous mesophase, ultimately leading to a more favorable alignment of the carbonaceous mesophase.
Protein-Aided Synthesis of Copper-Integrated Polyaniline Nanocomposite Encapsulated with Reduced Graphene Oxide for Highly Sensitive Electrochemical Detection of Dimetridazole in Real Samples
Authors: Behera Kartik , Mutharani Bhuvanenthiran , Chang Yen-Hsiang , Kumari Monika , Chiu Fang-Chyou
Published: January 2024

Dimetridazole (DMZ) is a derivative of nitroimidazole and is a veterinary drug used as an antibiotic to treat bacterial or protozoal infections in poultry. The residues of DMZ cause harmful side effects in human beings. Thus, we have constructed a superior electrocatalyst for DMZ detection. A copper (Cu)-integrated poly(aniline) (PANI) electrocatalyst (PANI-Cu@BSA) was prepared by using a one-step method of biomimetic mineralization and polymerization using bovine serum albumin (BSA) as a stabilizer. Then, the synthesized PANI-Cu@BSA was encapsulated with reduced graphene oxide (rGO) using an ultrasonication method. The PANI-Cu@BSA/rGO nanocomposite had superior water dispersibility, high electrical conductivity, and nanoscale particles. Moreover, a PANI-Cu@BSA/rGO nanocomposite-modified, screen-printed carbon electrode was used for the sensitive electrochemical detection of DMZ. In phosphate buffer solution, the PANI-Cu@BSA/rGO/SPCE displayed a current intensity greater than PANI-Cu@BSA/SPCE, rGO/SPCE, and bare SPCE. This is because PANI-Cu@BSA combined with rGO increases fast electron transfer between the electrode and analyte, and this synergy results in analyte–electrode junctions with extraordinary conductivity and active surface areas. PANI-Cu@BSA/rGO/SPCE had a low detection limit, a high sensitivity, and a linear range of 1.78 nM, 5.96 μA μM−1 cm−2, and 0.79 to 2057 μM, respectively. The selective examination of DMZ was achieved with interfering molecules, and the PANI-Cu@BSA/rGO/SPCE showed excellent selectivity, stability, repeatability, and practicability.
Integral Valorization of Posidonia oceanica Balls: An Abundant and Potential Biomass
Authors: Mnafki Rim , Morales Amaia , Sillero Leyre , Khiari Ramzi , Moussaoui Younes , Labidi Jalel
Published: January 2024

Posidonia oceanica balls (POB), a kind of seagrass, are a significant environmental issue since they are annually discharged onto beaches. Their current usefulness limits interest in their management and enhances the environmental problem. Therefore, in this research, the potential of this lignocellulosic biomass was studied from a holistic biorefinery point of view. To this end, an in-depth study was carried out to select the best pathway for the integral valorization of POBs. First, an autohydrolysis process was studied for the recovery of oligosaccharides. Then, a delignification stage was applied, where, in addition to studying different delignification methods, the influence of the autohydrolysis pre-treatment was also investigated. Finally, cellulose nanofibers (CNFs) were obtained through a chemo-mechanical treatment. The results showed that autohydrolysis not only improved the delignification process and its products, but also allowed the hemicelluloses to be valorized. Acetoformosolv delignification proved to be the most successful in terms of lignin and cellulose properties. However, alkaline delignification was able to extract the highest amount of lignin with low purity. CNFs were also successfully produced from bleached solids. Therefore, the potential of POB as a feedstock for a biorefinery was confirmed, and the pathway should be chosen according to the requirements of the desired end products.
Regulation of Hippocampal GABAergic Transmission by Fluoxetine and Its Metabolite Norfluoxetine
Authors: Vázquez-Gómez Elizabeth , Hernández-Abrego Andy , Mejía-Piedras Jassiel , García-Colunga Jesús
Published: January 2024

Major depression is related to dysfunction of the GABAergic pathway. Interestingly, the antidepressant fluoxetine modifies GABAergic neurotransmission in human and animal models of depression. However, the effects of norfluoxetine (the main metabolite of fluoxetine) on GABAergic neurotransmission have not yet been studied. Therefore, we explored whether fluoxetine and/or norfluoxetine may regulate GABAergic transmission and whether these substances interact with GABAA receptors in hippocampal CA1 stratum radiatum interneurons. For these purposes, we recorded the firing profile, GABAergic spontaneous inhibitory postsynaptic currents (sIPSCs), and currents induced by GABA puffs in stratum radiatum interneurons using both whole-cell current- and voltage-clamp techniques. Interneurons were selected according with their high firing profile. We found that both fluoxetine and norfluoxetine (at 20 µM) significantly decreased the frequency of sIPSCs without modifying their amplitude and decreased the amplitude of GABA-induced currents. These results indicate that fluoxetine and norfluoxetine decrease GABA release from neurons contacting stratum radiatum interneurons and negatively modulate GABAA receptors in these interneurons, resulting in their disinhibition, which in turn may contribute to increasing the inhibition of hippocampal CA1 pyramidal neurons.
From Ecotheology to Ecospirituality in Laudato sí—Ecological Spirituality beyond Christian Religion
Authors: Messias Teresa
Published: January 2024

This article discusses the notion of ecotheology, its origins and the conceptual framework of meaning, particularly within Christian theology, in order to establish its relation to the notion of ecospirituality. The article researches how ecological theology may ground an ecological spirituality. Then, it uses both notions to (1) analyze Pope Francis’ Encyclical Letter Laudato sí, (2) identify what models of ecotheology are used in his approach, (3) trace significant ecotheological dimensions, proposals, practices and lifestyles conveyed, (4) investigate if it is possible to identify the presence of an ecospirituality, concluding affirmatively. Finally, it asks whether Laudato sí’s ecospirituality is only meaningful and operative within a Christian spiritual framework or if, in many respects, it may be also inspirational and significant beyond a Christian realm of meaning. It concludes that Laudato sí’s ecological spirituality offers common ground for synergies with other religious, non-religious and secular ecospiritualities which can be synergic contributors to the creation of networks of action and meaning that can be implemented in order to develop a common passionate and effective protection of our world on environmental matters. The methodology used is a critical and hermeneutical documental analysis by means of reviewing significant literature in this area of knowledge.
Chinese Philosophical Practice toward Self-Cultivation: Integrating Confucian Wisdom into Philosophical Counseling
Authors: Ding Xiaojun , Fu Sirui , Jiao Chengcheng , Yu Feng
Published: January 2024

Traditional Chinese philosophy has always paid much attention to the cultivation of one’s body and mind, speeches and behaviors. The contemporary rise of philosophical practice, especially in the context of philosophical counseling, presents an opportunity to integrate the rich legacy of Confucian self-cultivation. This paper endeavors to delineate the practical applications of Confucian principles in philosophical counseling, with special emphasis on the actionable concepts of “gongfu” and “jingjie” and their synthesis in the “unity of knowledge and action”. Through a series of illustrative examples and proposed session outlines, we offer a vivid portrayal of how Confucian thought can be operationalized within a counseling framework to address the existential and ethical dilemmas faced by individuals in ancient and modern times. We argue that the nuanced understanding and practical implementation of these Confucian concepts can significantly enhance the efficacy of philosophical counseling. Additionally, we outline the requisite qualifications for becoming a Confucian philosophical counselor, suggesting a certification process that ensures counselors are both philosophically adept and exemplars of moral cultivation. Thus, this paper not only expounds on the theoretical underpinnings of Confucian thought in philosophical practice but also provides a clear blueprint for its application in contemporary settings.
Factors Associated with Uptake of No-Cost Safety Modifications to Home Access Steps: Implications for Equity and Policy
Authors: Keall Michael D. , Pierse Nevil , Cunningham Chris W. , Baker Michael G. , Bierre Sarah , Howden-Chapman Philippa
Published: January 2024

(1) Background: Fall injuries in the home present a major health burden internationally for all age groups. One effective intervention to prevent falls is home modification, but safety is only increased if opportunities to install safety modifications are taken up. This study sought to identify factors that may lead to a higher uptake of no-cost home modifications when these are offered to people living in the community. (2) Methods: We studied 1283 houses in the New Zealand cities of New Plymouth and Wellington. Using logistic regression, we modelled the odds of uptake according to the number of access steps, the provider who was undertaking the modifications, occupant ethnicity, prior fall injury history, and age group. (3) Results: Homes with older residents (age 65+) had higher uptake rates (35% vs. 27% for other homes). Larger numbers of access steps were associated with higher uptake rates. There was indicative evidence that homes with Indigenous Māori occupants had a higher uptake of the modifications for one of the two providers, but not the other. (4) Conclusions: No-cost home safety modifications offered via cold calling are likely to have relatively low uptake rates but the households that do consent to the modifications may be those who are more likely to benefit from the concomitant increased safety.
Migrant Perceptions of Their Social Inclusion, Social Networks, and Satisfaction with Life in Northern Spain
Authors: Roman Etxebarria Gorka , Berasategi Sancho Naiara , Idoiaga-Mondragon Nahia , Legorburu Fernandez Idoia
Published: January 2024

This paper aims to analyse the individual perceptions of belonging to social networks among migrants living in northern Spain, exploring various dimensions such as perceived inclusion and life satisfaction. A quantitative analysis was employed with data collected through a survey of 373 migrants from different ethnic backgrounds. The findings indicate that (1) women have higher levels of perceived satisfaction with their life and social networks; (2) young migrants have higher levels of friendship networks; (3) the highest levels of perceived inclusion were found among Central Europeans, followed by individuals from Latin America, Asia, Africa and, finally, Eastern Europe; and (4) each social network under analysis was positively correlated with perceived inclusion and satisfaction with life. In summary, the results emphasise that a greater presence of networks is associated with higher levels of perceived inclusion and life satisfaction.
The Shifting Stress of Working Parents: An Examination of Dual Pandemic Disruptions—Remote Work and Remote Schooling
Authors: Fan Wen , Moen Phyllis
Published: January 2024

Working remotely at least some of the time has long been seen as promoting a better integration of work and care obligations, even though prepandemic research is mixed as to the extent to which parents benefit emotionally from remote work. We exploit dual social experiments in schooling and work spawned by the COVID-19 pandemic to understand any stress-reducing effects of working from home under different school-closing state policy contexts. The pandemic led to an unprecedented shift to (and subsequent away from) remote and hybrid work but also to the implementation of various containment policies, most notably school closures driving a shift to remote learning that were put into effect to different degrees across U.S. states. Drawing on parents’ data from a U.S. nationally representative panel survey of workers who spent at least some time working from home since the pandemic onset, we use mixed-effects models to examine whether and in what ways cross-state and over-time variations in school closure policies shape any stress-reducing impacts of remote/hybrid work. Results show that when schools were not mandated to close, remote/hybrid work largely reduces parents’—especially mothers’—stress. However, an opposite pattern emerges in the face of closing mandates. These patterns are especially pronounced among white mothers and are not observed among nonparents.
Transforming Diabetes Supplies in the Prison System: An Example of Environmental Social Innovation
Authors: Moura Pedro Henrique Macedo , Silva Deise Maria Rego Rodrigues , Silva Eloia Emanuelly Dias , de Souza Jessiane Bispo , Barreto Marina dos Santos , Santos Ronaldy Santana , de Jesus Pamela Chaves , Santos Leticia Milena Machado dos , Santana Lucas Alves da Mota , Guimarães Adriana Gibara , Borges Lysandro Pinto
Published: January 2024

Background: The disposal of plastic material has been gaining negative attention due to its impact on the environment and people’s health. Insulin pens used by people living with diabetes are disposed of through landfills or incineration, negatively impacting the environment and generating costs for the state. Methods: In an attempt to reduce the disposal of plastic in the environment and reduce disposal costs, the “Insulinadiamor” project was created, which uses handicrafts as a means of re-socializing women deprived of their liberty. In this project, female inmates in the state of Sergipe turn insulin pens into ballpoint pens. Results: During the year 2022, 2000 insulin pens were transformed into ballpoint pens. We estimate a loss of around USD 37,150.28 for the state due to the inappropriate use of medicines, which was reflected in the return of complete insulins. In addition, by avoiding landfill or incineration of the plastic content collected, we contributed to an estimated saving of almost USD 603.91 for the state and more than 80 kg of CO2-equivalent emissions. Conclusions: This project brings a sustainable and transformative approach involving financial savings, rehabilitation of inmates, and contribution to the environment. It also raises the importance of creating more sustainable initiatives to reduce plastic waste in the health sector and other areas.
The Sustainable Production and Well-Being of Employees as a Derivative of the Concept of Sustainable Production
Authors: Niewiadomski Przemysław , Stachowiak Agnieszka
Published: January 2024

The main purpose of this work is to indicate the effects of the implementation of the concept of sustainable production from the perspective of the employee’s well-being. The subject of the research was manufacturers operating in the agricultural technical means of transport sector (production of parts and subassemblies). The research leads to the identification of benefits from the well-being of employees resulting from the implementation of the concept of sustainable production, described in the literature, that should be included in the evaluation model in the analysed sector (research question 1). Moreover, it enables the recognition of benefits resulting from the well-being of employees in the surveyed enterprises (level of fulfilment) and shortcomings highlighted by the companies surveyed in the category of benefits resulting from the well-being of employees (low level of fulfilment). The results of this work are the list of benefits structured into categories and assessed by the representatives of agricultural means of transport manufacturers. The list is useful from both academic and utilitarian perspectives.
Does the Energy-Consumption Permit Trading Scheme Improve Carbon Emission Performance? Evidence from a Quasi-Natural Experiment in China
Authors: He Qi , Jiang Hongli
Published: January 2024

The Chinese government launched the Energy-Consumption Permit Trading Scheme (ECPT) in 2016 as a pilot policy initiative to achieve energy conservation and emissions reduction goals. To generate a quasi-natural experiment, this study used a difference-in-differences (DID) approach with panel data from 2006 to 2020 in China. The goal was to explore the impact of the ECPT policy on the carbon emission performance (CEP) as well as analyze the underlying influence processes. The findings indicate that applying the ECPT has a considerable positive influence on the CEP. In comparison to provinces that did not engage in the pilot program, those that did reported a 4.5% improvement in CEP. We can safely declare that the results remain consistent and trustworthy after undergoing a battery of rigorous tests to assess the robustness of our findings. The mechanism’s evaluation revealed that the ECPT has the potential to improve the CEP, mostly via changing energy consumption patterns and stimulating technological innovation. Furthermore, when we considered heterogeneity, we discovered that factors such as geographical location, the level of green financing development, and the level of environmental regulation may affect the ECPT policy effects.
Chemo-Enzymatic Synthesis of Bioactive Carbazole Derivatives
Authors: Alrashdi Saad , Casolari Federica , Kyeremeh Kwaku , Deng Hai
Published: January 2024

Carbazoles are key scaffolds of either antimicrobial/antiviral alkaloid natural products or therapeutics. As such, access to structurally diverse indole-containing carbazoles has attracted considerable attention. In this report, a pilot study is described using biotransformation to provide carbazoles that contain various acyl substituents. The biotransformation system contains the thiamine-diphosphate (ThDP)-dependent enzyme NzsH, the FabH-like 3-ketoacyl-ACP synthase NzsJ, and the aromatase/cyclase NzsI, encoded in the biosynthetic gene cluster (nzs) of the bacterial carbazole alkaloid natural product named neocarazostatin A. The utilization of a range of acyl-SNACs (synthetic acyl-thioester analogues of the native substrate) together with indole-3-pyruvate and pyruvate in the designed biotransformation system allows production of carbazole derivatives. Our results demonstrate that this three-enzyme system displays a considerable substrate profile toward acyl donors for production of carbazoles with different acyl substituents. Finally, two more enzymes were included in the biotransformation system: the tryptophan synthase stand-alone β-subunit variant, PfTrpB, generated from directed evolution in the literature, and a commercially available L-amino acid oxidase (LAAO). The addition of these two enzymes allows the transformation to start with indole building blocks to provide carbazoles with modifications in the indole ring system.
Generation of New Glycoanalogues of Polyene Antibiotics by Synthetic Biology—Testing Current Technical Boundaries
Authors: Hogan Mark , Song Yuhao , Muldoon Jimmy , Caffrey Patrick
Published: January 2024

A number of antifungal drugs are based on polyene macrolides that cause severe side effects. Most of these compounds contain a single aminodeoxysugar, D-mycosamine. Toxicity can be reduced by increasing the extent of glycosylation. The aromatic heptaene 67-121C and two analogues of the degenerate heptaene nystatin have a second sugar attached to the C4′ hydroxyl of mycosamine. Another nystatin analogue has L-digitoxose as a second sugar attached to C35 on the macrolactone ring. The pentaene selvamicin has 4-O-methyl-L-digitoxose at C27, the equivalent position. To assist the production of new antifungals by synthetic biology, we explore further the utility of three classes of polyene glycosyltransferase: extending glycosyltransferases that form disaccharide-containing polyenes, glycosyltransferases that add the L-digitoxose sugars of nystatin A3 and selvamicin, and mycosaminyltransferases that add the primary aminodeoxysugar. In addition, we combine enzymatic hyperglycosylation with a known chemical method for adding sugars to the C3′ amino group of mycosamine. This was used to convert the disaccharide-containing 67-121C heptaene to forms containing branched trisaccharide or tetrasaccharide chains. These analogues are of interest for testing as anti-Leishmania drugs.
Sensory Considerations for Emerging Textile Applications
Authors: Kay Emma , Levick Jessica , Machingura Tawanda , Bird Stephen
Published: January 2024

Textiles are increasingly playing a role as a therapeutic medium in the disability field as well as in everyday life. This paper aims to review the literature on the relationship between textiles and sensory integration or sensory preferences in the general population. A brief literature review was conducted using PubMed (MEDLINE), SCOPUS, and Google Scholar. The review of the current literature highlights some key themes in the literature, including the indication that adaptive and sensory clothing design requires consideration of the textiles and fabrics being used, the functionality and appearance of garments, and affordability and access. The evidence suggests that clothing design should utilize soft fabrics which are seamless, have limited external tags, support social participation and functional engagement in daily activities, and are accessible.
Safe Disposal of Accident Wastewater in Chemical Industrial Parks Using Non-Thermal Plasma with ZnO-Fe3O4 Composites
Authors: Li Aihua , Wang Chaofei , Qian Chengjiang , Wen Jinfeng , Guo He
Published: January 2024

Chemical wastewater has a high concentration of toxic and hazardous antibiotic pollutants, which not only devastates the ecological environment and disrupts the ecological balance, but also endangers human health. This research proposed a non-thermal plasma (NTP) combined with a ZnO-Fe3O4 nano-catalyst system to achieve the efficient degradation of ciprofloxacin (CIP) in chemical wastewater. Firstly, ZnO-Fe3O4 composite materials were prepared using hydrothermal method and characterized with scanning electron microscopy (SEM), transmission electron microscope (TEM), X-ray diffractometer (XRD), X-ray photoelectron spectroscopy (XPS), etc. With the sole NTP, NTP/ZnO, and NTP/ZnO-Fe3O4 systems, the removal efficiency of CIP can reach 80.1%, 88.2%, and 99.6%, respectively. The optimal doping amount of Fe3O4 is 14%. Secondly, the capture agent experiment verified that ·OH, ·O2−, and 1O2 all have a certain effect on CIP degradation. Then, liquid chromatography–mass spectrometry (LC-MS) was used to detect the intermediate and speculate its degradation pathway, which mainly included hydroxyl addition, hydroxyl substitution, and piperazine ring destruction. After treatment with the NTP/ZnO-Fe3O4 system, the overall toxicity of the product was reduced. Finally, a cyclic experiment was conducted, and it was found that the prepared ZnO-Fe3O4 catalyst has good reusability. The NTP/ZnO-Fe3O4 was also applied in practical pharmaceutical wastewater treatment and has practical applicability.
Pro-Inflammatory and Anti-Inflammatory Interleukins in Infectious Diseases: A Comprehensive Review
Authors: Al-Qahtani Arwa A. , Alhamlan Fatimah S. , Al-Qahtani Ahmed Ali
Published: January 2024

Interleukins (ILs) are signaling molecules that are crucial in regulating immune responses during infectious diseases. Pro-inflammatory ILs contribute to the activation and recruitment of immune cells, whereas anti-inflammatory ILs help to suppress excessive inflammation and promote tissue repair. Here, we provide a comprehensive overview of the role of pro-inflammatory and anti-inflammatory ILs in infectious diseases, with a focus on the mechanisms underlying their effects, their diagnostic and therapeutic potential, and emerging trends in IL-based therapies.