bluuebunny/crossref_metadata_2025 huggingface dataset:
Uczniowie jednego Mistrza. Klasyczne instytucje postępowania administracyjnego w dobie przemian
Authors: Adamiak Barbara , Bąkowski Tomasz , Bogusz Mariusz , Chlebny Jacek , Chmielarz-Grochal Anna , Dańczak Paweł , Dębski Maciej , Dolnicki Bogdan , Duniewska Zofia , Furmanek Tomasz , Jagielski Jacek , Ciesielska Agnieszka , Wyporska-Frankiewicz Joanna , Sułkowski Jarosław , Wierzbowski Marek , Jagodziński Waldemar , Olechniewicz Elżbieta , Ziółkowski Dawid , Jaśkowska Małgorzata , Kabat Andrzej , Kijowski Dariusz R. , Knysiak-Sudyka Hanna Judyta , Kopacz Marta , Korzeniowski Piotr , Sieniuć Magdalena , Król Monika A. , Łaszczyca Grzegorz , Matan Andrzej , Maszewski Łukasz , Rączka Piotr , Mikołajczyk Joanna , Nędzarek Andrzej , Niczyporuk Janusz , Nykiel Włodzimierz , Piątek Wojciech , Romańska Marta , Sawuła Robert , Żukowski Ludwik , Skoczylas Andrzej , Skotnicki Krzysztof , Skóra Agnieszka , Sobieralski Krzysztof , Żywicka Agnieszka , Wilbrandt-Gotowicz Martyna , Zimmermann Jan
Published: April 2024

Niniejsza Księga jubileuszowa powstała dzięki wsparciu Partnerów: Okręgowej Izby Radców Prawnych w Łodzi, Okręgowej Rady Adwokackiej w Łodzi, Krajowej Izby Doradców Podatkowych i Izby Notarialnej w Łodzi. Partnerzy wydania przekazali nadto Jubilatom listy gratulacyjne z życzeniami zdrowia, wszelkiej pomyślności, a także wielu dalszych sukcesów zawodowych. Wyrażając uznanie dla stanowiącego nieocenioną pomoc w wykonywaniu zawodów prawniczych dorobku naukowego Panów Profesorów, a także dla Ich twórczego wkładu w kształtowanie orzecznictwa sądów administracyjnych, złożyli podziękowania za pasję naukową i społecznikowską, za trud nauczania młodych adeptów sztuki prawniczej, za podejmowanie działań na rzecz integrowania środowiska naukowego z ludźmi pracującymi w zawodach prawniczych i ekonomicznych, za tworzenie atmosfery sprzyjającej współpracy różnych grup prawników, za sympatię okazywaną samorządom zawodów prawniczych. Komitet Organizacyjny Jubileuszu składa Partnerom serdeczne podziękowania za okazaną pomoc i wsparcie.
Monilia and Monilinia : In silico genetic analysis of plant pathogenic fungi
Authors: Gyulai Gábor Zs. , Malone Renée P. , Gyulai Gábor , Ali Ajmal M. , Tóth Zoltán
Published: April 2024

Species of phytopathogenic fungi Monilia [J. Hill, 1751] and Monilinia [E.E. Honey, 1936] of the phylum Ascomycota have two host plant families of stone- and pome fruit trees of Rosaceae; and bushes of Ericaceae (e.g., blueberry, cranberry, heather, etc.). They infect both plants, and human (i.e., moniliasis). Due to the lack of sexual developmental stage (i.e., imperfect fungi; anamorphic fungi) the species of the order Moniliales spread by vegetative spores (i.e., conidia) and not by sexual (i.e., meiotic) ascospores. Here we report in silico data mining carried out by analyses of DNA, RNA, and protein sequences to reveal genetic distances in -and-among Monilia and Monilinia species. Sequences of the (+)ssRNA genomes of mitoviruses, which hyper parasitize the fungi cells, which parasitize the plant cells, during the ecocycles, are also analyzed. Genome size analysis of Monilinia were found to be between 30-55 x 106 bp; the cytochrome-b (cytb) genes (1543 bp of mtDNA); the transcribed cytb-mRNAs; the translated cytb proteins (381-391 aa); and the (+)ssRNA genomes of mitoviruses have showed that Botrytis cinerea (‘grey mold’) has the closest molecular similarity to Monilia and Monilinia clade (‘brown rot’). The cladogram of transcribed cytb-mRNAs (1175 nt) grouped Monilinia vaccinii-corymbosi in a distinct clade, which indicate a far genetic linkage. The application of mitovirus-infected hypovirulent phytopathogenic fungi for plant protection (i.e., virocontrol / biocontrol) is discussed.
A small garage on the sidelines of a corporation: Should innovative projects be isolated?
Authors: Kamiński Robert , Rosłoń Robert
Published: April 2024

The article aims to attempt to answer the question of whether innovative projects should be isolated in an organisation. A critical analysis of the literature on the subject reveals a divergence of views on the proper placement of social or technological innovation projects. The research hypothesis formulated holds that the more conducive the innovative project team is to interact with the rest of the organisation and the norms and values within the team are oriented toward learning, the stronger the learning of the project team. The culture metaphor of an organisation was used to verify the research hypothesis. It has been assumed that project team learning means that project team members transform the norms and values previously adhered to in the organisational culture. On the basis of the survey results, it was impossible to accept the research hypothesis. Despite the increase in the intensity of the factors that foster interactions between teams implementing innovative projects and the rest of the organisation, team learning did not increase as originally expected.
Consumers of different generations towards innovative technologies in customer service in retail
Authors: Kucharska Barbara
Published: April 2024

The aim of the paper is to identify the importance of innovative technologies used in retail during the pandemic for consumers and the willingness of different generations of consumers to use these solutions in the future. The article was prepared on the basis of the literature on the subject, research reports, websites of companies preparing or implementing modern technologies in retail, and the results of direct research conducted in 2022 using the online survey technique among 1,100 consumers.

The paper presents the issues related to the possibility of using modern technologies in the customer service process, specifically those that allow for even greater customer involvement in the purchasing process and increase their independence and self-service. A special emphasis was given to the self-service store, currently considered one of the greatest achievements (“milestones”) in brick-and-mortar retail trade, which is crucial for the issues presented. The paper shows the results of direct research on selected elements of purchasing behavior of different generations of consumers that are related to the use of innovative solutions in retail trade and intentions to use these solutions in the future.
DESENVOLVIMENTO DE UM SISTEMA PARA O MONITORAMENTO DO OXIGÊNIO DISSOLVIDO E DA PRESSÃO EM UM BIORREATOR DE BANCADA
Authors: Wagster Carlos Ataide Tavares  , Crema Cruz Leandra Cristina , Cruz Pedro Alexandre da
Published: April 2024

O Arduino é uma plataforma aberta e de baixo custo que permite a interação com dispositivos que apresentam diferentes funcionalidades, como sensores, módulos, entre outros. Para o monitoramento de um biorreator de bancada, sensores de oxigênio, pH, pressão, temperatura, entre outros são exemplos de dispositivos que podem ser utilizados em bioprocessos. O microcontrolador Arduino UNO R3 foi utilizado para a criação de um sistema para a obtenção de dados de oxigênio dissolvido e de pressão no biorreator. Foram desenvolvidos um circuito eletrônico e rotinas para a calibração dos sensores, assim como um código de programação que permitisse a obtenção e o armazenamento dos dados provenientes dos sensores. Um esboço foi elaborado pelo Autodesk AutoCAD para desenvolver um aparato experimental para a calibração do sensor transdutor de pressão, que juntamente com um manômetro padrão formarão um único sistema interligados recebendo as mesmas informações de pressão exercida na coluna de líquido. A metodologia empregada para calibração do sensor de oxigênio dissolvido demonstrou ser eficaz. O código de programação desenvolvido para o sensor de pressão será validado a partir de experimentos utilizando o aparato proposto no presente trabalho.
MICROBIAL FUEL CELLS AS AN ALTERNATIVE FOR THE TREATMENT OF WASTEWATER IN CONNECTION WITH ENERGY GENERATION
Authors: Freitas Fábio Rodrigo , Souza Elki Cristina
Published: April 2024

Concerns over environmental protection have increased in recent years, leading to a search for new renewable energy sources for minimizing anthropological damage to both atmosphere and water bodies. Microbial Fuel Cells are inserted in such a context, since they can reduce the organic load of an effluent concomitantly with the production of bioelectricity. This study investigated three different sources of microorganisms, evaluating parameters such as carbon source concentration and temperature in energy efficiency. The electrical current generated by microbial activity in the oxidation of organic matter was monitored additionally with ionic conductivity and pH of the medium. Chemical Oxygen Demand was also determined towards an evaluation of the removal of organic matter. The Microbial Fuel Cell inoculated with Activated Sludge showed higher electrical current in comparison to other studies from the literature and a greater generation of electrical current and a high influence of conductivity on that efficiency was observed at 36 °C. As a conclusion, Microbial Fuel Cells can operate at both 4.0 gL-1 (COD mgO2 3,886.728) and 3.0 gL-1 (COD mgO2 3,124.573).
Assembly Theory of Binary Messages
Authors: Łukaszyk Szymon , Bieniawski Wawrzyniec
Published: April 2024

Using assembly theory, we investigate the assembly pathways of binary strings of length N formed by joining bits present in the assembly pool and the strings that entered the pool as a result of previous joining operations. We show that the string assembly index is bounded from below by the shortest addition chain for N, and we conjecture about the form of the upper bound. We define the degree of causation for the minimum assembly index and show that for certain N it features regularities that can be used to determine a shortest addition chain. We show that a string with the smallest assembly index for N can be assembled by a binary program of length equal to this index if the length of this string is expressible as a product of Fibonacci numbers. We conjecture that there is no binary program that has a length shorter than the length of the string having the largest assembly index for N that could assemble this string. The results confirm that four Planck areas provide a minimum information capacity that corresponds to a minimum thermodynamic (Bekenstein-Hawking) entropy. Knowing that the problem of determining the assembly index is at least NP-complete, we conjecture that this problem is NP-complete, while the problem of creating the string so that it would have a predetermined largest assembly index is NP-hard. The proof of this conjecture would imply P ≠ NP, since every computable problem and every computable solution can be encoded as a finite binary string.
Could SP-A and SP-D Serum Levels Predict COVID-19 Severity?
Authors: Maddaloni Luca , Zullino Veronica , Bugani Ginevra , Lazzaro Alessandro , Brisciani Matteo , Mastroianni Claudio , Santinelli Letizia , Ruberto Franco
Published: April 2024

Background: given the various clinical manifestations that characterize coronavirus disease 2019 (COVID-19), the scientific community is constantly searching for biomarkers with prognostic value. Surfactant protein A (SP-A) and D (SP-D) play a crucial role in ensuring proper alveolar function and an alteration of their serum levels have been reported in several pulmonary diseases characterized by acute respiratory distress syndrome (ARDS) and pulmonary fibrosis. Consid-ering that such clinical manifestations can also occur during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, we wondered if these collectins could act as prognostic markers. Methods: in this regard, serum levels of SP-A and SP-D were measured by enzyme immunoassay in patients with SARS-CoV-2 infection (n=51) at admission (T0) and after 7 days (T1) and compared with healthy donors (n=11). Results: SP-D increased in COVID-19 patients com-pared to healthy controls during the early phases of infection, while a significant reduction was observed at T1. Stratifying SARS-CoV-2 patients according to disease severity, increased serum SP-D levels were observed in severe compared to mild patients. Conclusions: In the light of these results SP-D, but not SP-A, seems to be an eligible marker of COVID-19 pneumonia and the early detection of SP-D serum levels could be crucial for a preventive clinical management.
Symphony in the Brain
Authors: Mikheenko Pavlo
Published: April 2024

Despite impressive progress in quantum biology, many questions about perception, cognition, memory, and their relation to processing of information remain unanswered. Here is an attempt to draw a unified picture starting with the basic quantum-mechanical phenomenon of nano-superconductivity in the microtubules and extending it to quantum processing of information, and generation of coherent Josephson radiation. A model for the generation in the axons of neurons is suggested. Like in a symphony, but on frequencies ten orders of magnitude higher, the radiation forms resonant standing electromagnetic waves, which are defined by the anatomy, synchronize all the functions in organism, and are behind the memory, perception, and cognition of a living being. It is true that we are made of atoms, but we are coherent electromagnetic waves.
Perspectives for Photocatalytic Decomposition of Environmental Pollutants and Pathogens on Photoactive Particles of Soil Minerals
Authors: Sosnowska Agnieszka , Hęclik Kinga I. , Kisała Joanna B. , Celuch Monika , Pogocki Dariusz
Published: April 2024

The literature shows that both in laboratory and in industrial conditions, photocatalytic oxidation method copes quite well with the degradation of the most organic substances, including environmental toxins and molecular components of pathogenic microorganisms. However, the effective utilization of photocatalytic processes for environmental decontamination and disinfection require significant technological advancement both in the area of semiconductor material synthesis and its application. Here we focused on the presence and ‘photocatalytic capability‘ of photocatalysts among soil minerals, and their potential contribution to the environmental decontamination in vitro and in vivo. (The issue is sketched from the perspective of chemists and environmental scientist.) Reactions caused by sunlight on the soil surface are involved in its normal redox activity. It appears that some of them may take part in the soil decontamination. However, their importance for decontamination in vivo cannot be overstated due to diversity of soils on the Earth caused by the environmental conditions, such as climate, parent material, relief, vegetation etc. The solar-light induced reactions are just a part of extremely complicated processes dependent on a plethora of environmental determinates. Such multiplicity of affecting factors, which we tried to sketch from the perspective of chemists and environmental scientist, makes us rather sceptical about the effectiveness of the photocatalytic processes in vivo. On the other hand, there is a huge potential of the soils as the alternative source of useful photocatalytic materials of unique properties
An Empirical Examination of Bitcoin’s Halving Effects: Assessing Cryptocurrency Sustainability within the Landscape of Financial Technologies
Authors: Fabuš Juraj , Kremeňová Iveta , Stalmašeková Natália , Kvasnicová-Galovičová Terézia
Published: April 2024

This article delves into the complex landscape of Bitcoin halving events, analyzing the historical context and implications of these pivotal occurrences within the cryptocurrency ecosystem and on its sustainability within the landscape of financial technologies. Through an examination of past halving events in 2012, 2016, and 2020, this study meticulously scrutinizes the conditions and prices of Bitcoin before and after each halving. By synthesizing data and trends from previous events, this article aims to uncover patterns and insights that illuminate the impact of Bitcoin halving on market dynamics and sustainability, movement of the price level, reached peaks and price troughs. Furthermore, this research presents a comparative analysis of past halving events, drawing parallels and distinctions to elucidate the evolving nature of Bitcoin's price movements and market sentiment surrounding these milestone events. Looking towards the future, this study estimates predictions and expectations for the upcoming Bitcoin halving. By unraveling the intricate relationship between Bitcoin halving events and market behavior, this article seeks to provide a comprehensive understanding of the broader implications for Bitcoin's trajectory and the evolving landscape of the cryptocurrency market and its sustainability.
Exploring the Impact of Maternal Gatekeeping on Paternal Parenting and Adolescent Aggression
Authors: Wang Huaiyu , Xu Peijing , Jiang Yali
Published: April 2024

Based on the traditional Chinese cultural belief of "male breadwinner, female homemaker," as well as the systemic and interactive characteristics of families, this study aims to explore the influence of maternal gatekeeping behavior on the quality and quantity of paternal parenting, as well as adolescent aggressive behavior. A total of 483 seventh-grade students completed questionnaires on maternal gatekeeping behavior, paternal involvement, parenting styles and aggressive behavior. Latent profile analysis identified four parenting combinations: positive, negative, mixed, and neglectful. Adolescents under negative parenting exhibited the highest aggression, while those under positive and neglectful parenting showed the least aggression. Maternal gatekeeping behavior correlated with paternal negative parenting and adolescent aggression. Paternal negative parenting mediated the relationship between maternal gatekeeping and aggression, while paternal involvement moderated this relationship. These findings highlight the role of parental interaction in adolescent behavior and support family-based interventions.
Optimization of Geothermal Heat Pump Systems for Sustainable Urban Development in Southeast Asia
Authors: Chanchayanon Thiti , Chaiprakaikeow Susit , Jotisankasa Apiniti , Inazumi Shinya
Published: April 2024

This study examines the optimization of ground source heat pump (GSHP) systems and energy piles for sustainable urban development, with a focus on Southeast Asia. GSHPs, which utilize geothermal energy for indoor HVAC needs, offer a sustainable alternative to traditional systems by utilizing consistent subsurface temperatures for heating and cooling. The study highlights the importance of understanding thermal movement within the soil, especially in soft marine clays prevalent in Southeast Asia, to improve GSHP system efficiency. Using a one-dimensional finite-difference model, the study examines the effects of soil thermal conductivity and density on system performance. The results show that GSHP systems, especially when integrated with energy piles, significantly reduce electricity consumption and greenhouse gas emissions, underscoring their potential to mitigate the urban heat island effect in densely populated areas. Despite challenges posed by the region's hot and humid climate, which could affect long-term effectiveness, the study highlights the need for further study, including field experiments and advanced modeling techniques, to optimize GSHP configurations and fully exploit geothermal energy in urban environments. The study's insights into soil thermal dynamics and system design optimization contribute to the advancement of sustainable urban infrastructure development.
Experimental and Numerical Investigation of the Use of Ultrasonic Waves to Assist Laser Welding
Authors: Salimi Mohamad , Teyeb Ahmed , Elmasri Evelyne , Hoque Samiul , Carr Phil , Balachandran Wamadeva , Gan Tat-Hean
Published: April 2024

This study explores the use of remote ultrasound vibration to enhance welding strength. It examines the effects of 20 kHz, and 40 kHz High Power Ultrasound Transducers (HPUT) in contact and contactless modes during ultrasonic assistance laser welding. Employing shear tests and microscopy for assessment, the research evaluates ultrasound's impact on welding strength and identifies the optimal transducer. Theoretical and numerical analyses investigate vibration transmission into the welding area, particularly examining the vibration distribution and cavitation initiation within the molten pool area. Results indicate that ultrasound-assisted welding increases pull test performance, including the resistance force and strain, and improves microscopic structural integrity. Numerical simulations suggest that narrow-band vibration intensifies pressure in specific welding zones. Contactless ultrasound application also shows enhanced welding quality, offering a promising alternative to current methods. This work contributes to electric car technology by suggesting improvements in welding techniques for power battery systems.
Influence of Canteens on the Eating Behavior of Private School Students
Authors: Lourenço Rayane Lorena Melo , Sá Clara Cecília Ribeiro , Moura Beatriz Gouveia , Sales Joana Medeiros , Borges Luiza Delazari , Silva José Rodrigo Santos , Mendes Larissa Loures , Fagundes Andhressa Araújo
Published: April 2024

The school food environment is a suitable place to promote healthy habits, as it is a space for learning and constant social interaction, where children spend at least a third of their day, and have one to two meals. This study aimed to analyze the sale of food in the canteens of private schools in a capital in the Northeast of Brazil and verify the influence of it on students’ eating behavior. A cross-sectional, quantitative, multicenter study was carried out, taking place simultaneously in twenty capitals in Brazil. A checklist with 87 questions was applied to those responsible for the canteens in a representative sample of schools in each city. Frequencies and associations were performed using Pearson's Chi-Square test. The analysis of the foods sold, was based on the NOVA food classification from the Dietary Guidelines for the Brazilian Population, regarding the degree and extent of processing. Latent Class Analysis was carried out to group the canteens into similar response patterns, modeled with covariates, and the association was calculated with the Kruskal-Wallis and Duun tests or Fisher's Exact test. The results showed that students were very frequently offered ultra-processed, sugar-rich foods with low nutritional value; 88.7% of such foods had been chosen by managers as part of the menu because they are “students’ food preferences”. There is an average of 2.3 fresh foods in canteens compared to 7.5 varieties of ultra-processed foods. There must be stricter control over what is sold, to minimize student’s exposure to unhealthy foods.
Mindfulness, Gut‐Brain Axis and Health Related Quality of Life: The Paradigm of IBD Patients
Authors: Christodoulou Efstratios , Mpali Tsambika , Dimitriadou Maroula-Eleni , Koutelidakis Antonios E.
Published: April 2024

Health-Related Quality of Life (HRQoL) encompasses the comprehensive evaluation of an indi-vidual's health and well-being across multiple dimensions. Mindfulness, recognized as a scientifi-cally supported strategy for anxiety management, plays a role in enhancing both physical and mental health. The Gut-Brain Axis (GBA) serves as the bidirectional communication link between the gastrointestinal system and the central nervous system. This study aims to explore the associa-tion between Mindfulness and factors influencing the GBA in both healthy individuals and those with Inflammatory Bowel Diseases (IBD). A cross-sectional investigation involved 338 adults, in-cluding 50 IBD patients, utilizing validated Greek scales for Mindfulness (MAAS-15), Mediterra-nean Diet (14-MEDAS), and HRQoL (EQ-5D-5L). The questionnaire gathered demographic, an-thropometric, and lifestyle data. Among healthy participants, EQ-5D-5L showed a moderate cor-relation with the MAAS-15 scale (r=0.389, p
Reliability Assessment of Wireless Sensor Networks by strain-based Region Analysis for Redundancy Estimation in Measurements on the Example of an Aircraft Wing Box
Authors: Meyer zu Westerhausen Sören , Raveendran Gurubaran , Lauth Thorben-Hendrik , Meyer Ole , Rosemann Daniel , Wawer Max Leo , Stauß Timo , Wurst Johanna , Lachmayer Roland
Published: April 2024

Wireless Sensor networks (WSNs) are gaining more and more research interest due to their ability to monitor large areas more or less independent. However, their reliability is an important issue, since their system reliability is influenced from a hardware, a data and an energetic point of view due to different factors like loading conditions, signal attenuation and battery lifetime. Regarding these issues, the positions of sensor nodes have to be chosen correctly to maximize the system reliability, which has to be considered during the development of new products equipped with WSNs. In this paper, an approach to estimate a WSNs system reliability during the development phase based on the analysis of the measurements on the example of strain measurements in finite elements (FE) models is presented. For that, the part under consideration is divided into regions with similar strains by the use of a Region Growing Algorithm (RGA). Afterwards, the WSN’s configuration is analyzed regarding its reliability in accordance to the data paths and measurement redundancy due to the sensor positions in the found measuring regions. This methodology is tested on an exemplary WSN configuration at an aircraft wing box under bending load and was found to work very well regarding the estimation of the hardware perspective on the system reliability. Therefore, the methodology and the developed algorithm show potentials for optimizations in sensor node positions for better reliability results.
Urban Sustainability in Unconventional Food, Case of Edible Insects in Kinshasa: Diversity and Entomophagy
Authors: Célestin ADEITO MAVUNDA , Kanda Madjouma , Folega Fousseni , BAWA Demirel Maza-esso , Dourma Marra , Akpagana Koffi
Published: April 2024

The consumption of insects in the Democratic Republic of Congo is an ancient practice. The taxonomic identification and characterization of “entomophagy” seem urgent in Kinshasa following the related anthropogenic pressures (demographic pressure, uncontrolled urbanization) leading to a food crisis. This study aims to assess the diversity of edible insects and their consumption by the population of Kinshasa. A total of 360 people were surveyed to identify the insect species consumed in Kinshasa, the ecosystems that provide them, and the preference for species in relation to ethnolinguistic groups, churches (faith) and household size. Three species of insects most consumed in Kinshasa are: Gonimbrasia jamesonii, Cirina forda and Brachytrupes membranaceus. 41.9% of these insects are consumed by the Kongo ethno-linguistic group, 23.6% by the Luba, 20.6% by the Ngala and 13.9% by the Swahili. Inhabitants of the communes of Kassa Vubu (57%), Maluku (54%), Masina (54%) and Mont-Ngafula (39%) mainly consume C. forda and G. jamesonii. Luba and Kongo ethnolinguistic groups mainly consume C. forda while the Swahili and Ngala consume G. jamesonii. Catholic, Revivalist Church, Kimbagu, Islam, Protestant and Johovah's Witness believers mainly consume C. forda and G. jamesonii. This study made it possible to understand the socio-cultural influence on the degradation (pressure) of biodiversity and; contributes to SDGs 2 and 12 by 2023..
TF-Target Finder: An R Web Application Bridging Multiple Predictive Models for Decoding Transcription Factor-Target Interactions
Authors: Wang Jin
Published: April 2024

Transcription factors (TFs) are crucial in modulating gene expression and sculpting cellular and organismal phenotypes. The identification of TF-target gene interactions is pivotal for comprehending molecular pathways and disease etiologies but has been hindered by the demanding nature of traditional experimental approaches. This paper introduces a novel web application, utilizing the R programming language, which predicts TF-target gene relationships and vice versa. Our application integrates the predictive power of various bioinformatic tools, leveraging their combined strengths to provide robust predictions. It merges databases for enhanced precision, incorporates gene expression correlation for accuracy, and employs pan-tissue correlation analysis for context-specific insights. The application also enables the integration of user data with established resources to analyze TF-target gene networks. Despite its current limitation to human data, it provides a platform for exploring gene regulatory mechanisms comprehensively. This integrated, systematic approach offers researchers an invaluable tool for dissecting the complexities of gene regulation, with the potential for future expansions to include a broader range of species.
SARS-CoV-2 and Influenza Co-Infection: Fair Competition or Sinister Combination?
Authors: Narasaraju Teluguakula , Chow Vincent TK , MD Pandareesh , Dasegowda Venkatesha , Kurrapotula Vidyasagar , MG Shivaramu , Radic Marko
Published: April 2024

The COVID-19 pandemic remains a serious public health problem globally. During winter flu seasons, more aggressive SARS-CoV-2 infections with fatalities have been documented, indicating that influenza co-infections may significantly impact the disease outcome of COVID-19. Both influenza and SARS-CoV-2 viruses share many similarities in their transmission and cellular tropism for their replication in the human respiratory tract. However, it is still unclear how the two pathogens interplay to ensure their survival in the same lung microenvironment. In addition, clinical studies on influenza co-infections in COVID-19 patients do not provide conclusive evidence on how influenza co-infection mechanistically modifies the disease outcome in COVID-19. This mini-review discusses various viral as well as host factors that potentially influence the survival or synergism of these two respiratory pathogens in the infected lung microenvironment.
Updated Gene Prediction of Cucumber Genome through Manual Annotation
Authors: Du Weixuan , Xia Lei , Li Rui , Zhao Xiaokun , Jin Danna , Wang Xiaoning , Pei Yun , Zhou Rong , Chen Jinfeng , Yu Xiaqing
Published: April 2024

Thorough and precise gene structure annotations are essential for maximizing the benefits of genomic data and unveiling valuable genetic insights. The cucumber genome was first released in 2009 and updated in 2019. To increase the accuracy of predicted gene models, 64 published RNA-seq data and 9 new strand-specific RNA-seq data from multiple tissues were used for manual comparison with the gene models. The updated annotation file (version 2.0) contains increased 24,145 predicted genes from previous version (24317 genes) with a higher BUSCO value of 96.9%. 6231 and 1490 transcripts were adjusted and newly added, respectively, accounting for 31.99% of the overall gene tally. These newly added and adjust genes were renamed (CsaV3.1_XGXXXXX), while genes remaining unaltered preserve their original des ignations. A random selection of 21 modified/added genes were validated using RT-PCR analyses. Additionally, tissue-specific patterns of gene expression were examined using newly obtained transcriptome data with the revised gene prediction model. This improved annotation of cucumber genome will provide essential and accurate resources for studies in cucumber.
Development of an Italian National Epidemiological Register on Endometriosis based on Administrative Data
Authors: Maraschini Alice , Ceccarelli Emiliano , Giangreco Manuela , Monasta Lorenzo , Manno Valerio , Catelan Dolores , Stoppa Giorgia , Biggeri Annibale , Ricci Giuseppe , Buonomo Francesca , Minelli Giada , Ronfani Luca
Published: April 2024

Background: Endometriosis is a female chronic inflammatory disease in which endometrial tissue develops outside the uterine cavity. It is a complex pathology, that significantly contributes to morbidity in premenopausal women, leading to chronic pain, infertility, and subfertility negatively impacting the physical, and emotional well-being and overall quality of life. The public health burden of endometriosis remains elusive and challenging to determine, and this uncertainty can lead to inadequate healthcare services and treatments. Objective: To estimate the incidence and prevalence of endometriosis in Italy using the hospital discharge records database. Population-based retrospective study. Nationwide between 2011 and 2020. Methods: From the National Hospital Discharge Database were selected all admissions with a diagnosis of endometriosis (ICD-9-CM, codes 617.x), supported by the presence of a procedure code of laparoscopy or any other surgical procedure allowing direct visualisation of the lesions. Main outcomes measures Incidence and prevalence of endometriosis were estimated for the entire 2010-2020 period and by individual year, analysing the time trend and variability in different geographical areas of Italy. Results: A total of 134,667,646 women aged 15–50 years with one or more hospitalisations for endometriosis in all Italian hospitals. The incidence of endometriosis in Italy during this period was 0.839 per 1000 women (CI95% 0.834-0.844), exhibiting a statistically significant decreasing trend over the years. A discernible north-south gradient was observed, with higher rates documented in the northern regions. The prevalence rate stood at 14.0 per 1000 during the same period, and a similar north-south geographical gradient was identifiable in the prevalence rates as well. Conclusions: The utilization of national-level hospital data enables the generation of incidence and prevalence data for endometriosis without variations in methods and definitions, facilitating the evaluation of temporal trends and regional comparisons. Understanding and quantifying this phenomenon is essential for appropriate healthcare planning in various Italian regions.
Finding a Direct method for a Dynamic process: the DD (Direct and Dynamic) Cell-Tox method
Authors: Madorran Eneko , Kocbek Šaherl Lidija , Rakuša Mateja , Munda Mihael
Published: April 2024

The main focus of the in vitro toxicity assessment methods is on the viability of the cells, which is usually based on metabolism changes. Yet, when exposed to toxic substances, the cell triggers multiple signals in response. With this in mind, we have developed a promising cell-based toxicity method that observes various cell responses when exposed to toxic substances (either death, division or remaining viable). Based on the collective cell response, we observed and predicted the dynamics of the cell population to determine the toxicity of the toxicant. The method was tested with two different conformations: In the first conformation, we exposed a monoculture model of blood macrophages to UV light, hydrogen peroxide, nutrient deprivation, tetrabromobisphenol A, fatty acids and 5-fluorouracil. In the second, we exposed a coculture liver model consisting of hepatocytes, hepatic stellate cells, Kupffer cells and liver sinusoidal endothelial cells to rifampicin, ibuprofen and 5-fluorouracil. The method showed good accuracy compared to established toxicity assessment methods. In addition, this approach provided more representative information on the toxic effects of the compounds as it considers the different cellular responses induced by toxic agents.
Isoquinoline Alkaloids Interaction With Plasma Proteins
Authors: Marciniak Aleksandra , Kotynia Aleksandra , Krzyżak Edward , Czyżnikowska Żaneta , Zielińska Sylwia , Kozłowska Weronika , Białas Marcel , Matkowski Adam , Jezierska-Domaradzka Anna
Published: April 2024

A comprehensive study of the interactions of human serum albumin (HSA) and α-1-acid glycoprotein (AAG) with two isoquinoline alkaloids: allocryptopine (ACP) and protopine (PP) was performed. The UV-Vis spectroscopy, molecular docking, competitive binding assays, and circular dichroism (CD) spectroscopy were used for the investigations. The results showed that ACP and PP form spontaneous and stable complexes with HSA and AAG, with ACP displaying a stronger affinity towards both proteins. Molecular docking studies showed also revealed the preferential binding of ACP and PP to specific sites within HSA, with site 2 (IIIA) being identified as the favored location for both alkaloids. This was supported by competitive binding assays using markers specific to HSA's drug binding sites. Similarly, for AAG, a decrease in fluorescence intensity upon addition of the alkaloids to AAG/ Quinaldine Red (QR) complexes indicated the replacement of the marker by the alkaloids, with ACP showing a greater extent of replacement than PP. CD spectroscopy showed that the proteins’ structures remained largely unchanged suggesting that the formation of complexes did not significantly perturb the overall spatial configuration of these macromolecules. These findings are crucial for advancing the knowledge on the natural products-protein interactions and the future design of isoquinoline alkaloid-based therapeutics.
From the Gut to the Brain: Is Microbiome a New Paradigm in Parkinson’s Disease Treatment?
Authors: Vilela Cristiana , Araújo Bruna , Soares-Guedes Carla , Caridade-Silva Rita , Martins-Macedo Joana , Teixeira Catarina , Gomes Eduardo D , Prudêncio Cristina , Vieira Mónica , Teixeira Fábio G.
Published: April 2024

Parkinson's disease (PD) is recognized as the second most prevalent primary chronic neurodegenerative disorder of the central nervous system. Clinically, PD is characterized as a movement disorder, exhibiting an incidence and mortality rate that is increasing faster than any other neurological condition. In recent years, there has been a growing interest concerning the role of the gut microbiome in the etiology and pathophysiology of PD. The establishment of a brain-gut microbiome axis is now real, with evidence denoting a bidirectional communication between the brain and the gut microbiota through metabolic, immune, neuronal, and endocrine mechanisms and pathways. Among these, the vagus nerve represents the most direct form of communication between the brain and the gut. Given the potential interactions between bacteria and drugs, it has been observed that the therapies for PD can have an impact on the composition of the microbiome. Therefore, in the scope of the present review, we will discuss the current understanding of gut microbiome on PD and whether this may be a new paradigm for treating this devastating disease.
Impact of Comorbidities and the Duration of Mechanical Venti-Lation on Outcomes of Surgical Patients with Isolated Candida Species in the Intensive Care Unit: A Cross-Sectional Study
Authors: Glavaš Tahtler Josipa , Kvolik Slavica
Published: April 2024

Abstract: Comorbidities of patients may be related to the duration of mechanical ventilation, survival, and Candida isolation during intensive care unit (ICU) stay. To examine the frequency of Candida isolation and significant comorbidities in the surgical intensive care unit (ICU) in Osijek University Hospital, Croatia, the data from the electronic database from May 2016 to June 30, 2023, were analyzed. In a cross-sectional study examining 15,790 microbiological samples, different strains of Candida were observed in 581 samples from 236 patients. The control group was 160 consecutive patients from March-May 2019, and 161 in the same months of 2020 (pre- and post-COVID-19). Comorbidities, duration of mechanical ventilation and survival were compared. Patients with isolated Candida were more often non-elective, had significantly more heart, kidney, liver diseases and sepsis (P<0.001). The duration of mechanical ventilation was 9.2 [2.2-9.24], 96 [24-146], 160 [19.5-343] and 224 [73.5-510] hours in controls, and patients with Candida albicans, non-albicans, and in patients with ³2 Candidas isolated. The mortality was significantly higher and reached 42% in patients with Candida isolated, while in the control group, it was 19% (P<0.001). In a multivariate analysis adjusted for patient’s age, SAPS II score, days of ICU, and type of admission, only sepsis was independent predictor of mortality (OR = 2.27).
Evaluation of Sentinel Lymph Nodes in Complex Atypical Endometrial Hyperplasia
Authors: Al Kallas Hala , Cooper Pamela , Varma Shruti , Peplinski Jenna , Kuo Yan Hong , Miller Brianna , Aikman Noelle , Borowsky Mark Eliot , Haggerty Ashley , ElSahwi Karim
Published: April 2024

Complex atypical endometrial hyperplasia (CAH) carries a high probability of cancer. Intraoperative evaluation of endometrial cancer in cases of CAH has not been reliable. The safety and sensitivity of sentinel lymph node (SLN) sampling has been validated. We aim to evaluate the efficacy and safety of SLN sampling in CAH managed by the da Vinci robotic platform. A total of 113 patients with a preoperative diagnosis of CAH were included in this retrospective cohort study. All underwent a robot assisted total laparoscopic hysterectomy and bilateral salpingo-oophorectomy with 69 patients undergoing SLN sampling. Statistical analysis calculated the probability of cancer, SLN map rate, and surgical complications. Predictors of cancer were evaluated. Forty-seven percent of the entire cohort were diagnosed with endometrial cancer. Median age was 63 years in the SLN cohort (N = 69) and 61 in the No SLN cohort (N = 44) (p= 0.363).  Median BMI was 34 Kg/m2 in the SLN cohort and 40 in the No SLN cohort (p= 0.004). Bilateral SLN map was 92.8% and unilateral SLN map rate was 7.2%. There were no grade 3-4 complications in the SLN cohort, and 4 grade 3-4 complications in the No SLN group (p=0.021). A preoperative diagnosis of CAH bordering on or cannot rule out cancer was the only predictor of cancer.
Supramolecular Systems Linked to Metal‐Organic Frameworks
Authors: Sánchez-Fernández José Antonio , Cue-Sampedro Rodrigo , Saade Hened
Published: April 2024

Supramolecular structures with specific applications are a pillar in several areas of science. Thus, from a contemporary point of view, there are several reasons to feel inclined to cover a systematic, or historic critique of the supramolecular concept itself. First, the idea of a supramolecular material seems more secure now than it was decades ago. Second, updating the interacting of MOFs and supramolecular chemical and conversely, supramolecularity to assemble MOFs and to make ef-ficient complex systems in multiple cutting-edge applications. Third, we should here simply confine to a consideration of how workers in this field have tried to correlate the notion of the idea of supramolecular systems linking self-assembly considerations. Thereby, this is clearly an ad-vantage that optimized geometries obtained on the conductive bases described that undoubtedly, some of these systems have substantial applications and create a significant practical interest. The outline of this review encompasses a general introduction to MOFs and supramolecularity, the key unit of the study presented here, followed by a survey of recent advances in supramolecular chemistry in confined space, relationships of MOFs with the supramolecular chemistry, electro-chemical synthesis of MOFs, and switchable MOFs. Finally, some discussion on possible future perspectives of this field is given.
2-Methoxyestradiol, an Endogenous 17β-Estradiol Metabolite, Induces Antimitogenic and Apoptotic Actions in Oli-Neu – Oligodendroglial Precursor Cells and Triggers Endoreduplication via p53 Pathway
Authors: Schaufelberger Sara. A. , Schaettin Martina , Azzarito Giovanna , Rosselli Marinella , Leeners Brigitte , Dubey Raghvendra Krishna
Published: April 2024

Abstract: Abnormal growth of oligodendrocyte precursor cells (OPCs) importantly contributes to the progression of glioblastoma tumors. Hence, molecules that block OPC growth may be of therapeutic importance in treating gliomas. 2-methoxyestradiol (2ME), an endogenous tubulin interacting metabolite of estradiol is effective against multiple proliferative disorders. Based on its anti-carcinogenic and anti-angiogenic actions it is undergoing phase II clinical trials. We hy-pothesize that 2ME may prevent glioma growth by targeting OPC growth. Here we tested this hypothesis by assessing the impact of 2ME on growth of OPC cell line “Oli-neu” and dissected the underlying mechanism(s). Treatment with 2ME inhibited OPC growth in a concentra-tion-dependent manner and accompanied with significant upregulation in expression of p21 and p27, negative cell cycle regulators. Moreover, treatment with 2ME altered OPC morphology from multi-arm processes to rounded cells. At concentrations of 1uM and greater 2ME induced apoptosis with increased expression of caspase 3, PARP and caspase-7 fragments, externalized phosphatidylserine staining / apopercentage and increased mitochondrial activity. Flow-cytometry and microscopic analysis demonstrated that 2ME triggers endoreduplication in a concentration-dependent fashion. Importantly, 2ME induced cyclin E, JNK1/2 and p53 expres-sion as well as OPC cell fusion, key mechanisms driving endoreduplication or whole-genome duplication. Importantly, inhibition of p53 with pfithrin-α rescued 2ME-induced endoreduplica-tion. The pro-apoptotic and endoreduplication actions of 2ME were accompanied with upregula-tion of survivin, cyclin A, Cyclin B, Cyclin D2, ppRB. Taken together our findings provide evi-dence that 2ME not only inhibits OPC growth and triggers apoptosis, but also activates OPCs in a survival - fight or flight mode leading to endoreduplication. This inherent survival character-istic of OPCs may, in part be responsible for drug resistance in gliomas as observed for many tubulin interacting drugs. Importantly, the fate of OPCs after 2ME treatment may depend on cell-cycle status of the individual cells. Combining tubulin-interfering molecules with drugs, such as pfithrin-α, that inhibit endoreduplication may help inhibit OPC/glioma growth and lim-it drug resistance.
A Comprehensive Review of Electric Vehicles Fast Charging Developments and Technology
Authors: Zentani Ahmed Taher , Almaktoof Ali M. A. , Kahn MTE
Published: April 2024

The electric vehicle (EV) industry is experiencing rapid growth, accompanied by continuous advancements in charging infrastructure to satisfy the rising need for fast and reliable charging. Particularly, the focus has shifted towards EV fast charging (EVFC) and its impact on the power grid. This paper presents a comprehensive analysis and survey of scholarly literature and projects conducted in this field over the past decade. The findings highlight the rising demand for EVFC and the need to address its potential adverse effects on the power grid. However, there is a noticeable lack of clear research guidance regarding charging time. To tackle these challenges and pave the way for future solutions, extensive research on DC fast charging, ultra-fast charging, and the integration of vehicle-to-grid (V2G) systems is required. This review paper thoroughly investigates the development of fast charging technology for electric vehicles (EVs), including its advantages and comparative analyses from various perspectives. Furthermore, it delves into the advancements in DC fast charging infrastructure, emphasizing charging standards and control modes. The study also investigates different categories of battery chargers for both on-board and off-board charging. Additionally, it explores the classification of DC-DC converters in the context of DC fast charging stations, discusses control strategies for EV systems, identifies existing challenges, and outlines future trends in EV fast charging.