bluuebunny/crossref_metadata_2025 huggingface dataset:
Technology-based CBT in Reducing Symptoms of OCD in Children: A Systematic Review
Authors: Samson Francine , Tafuto Barbara , Jose Nadina , Kim Lisa Palladino
Published: September 2021

Background: Obsessive Compulsive Disorder (OCD) is an anxiety disorder affecting up to 3% of children and adolescent in the United States. Cognitive behavioral therapy (CBT) is the first-line treatment for mild to moderate OCD in children. Despite the benefits of CBT in treatment of OCD, only one-third of clinicians regularly use CBT compared to psychotropic medication due to lack of access. The COVID-19 pandemic has resulted in increased relapses in OCD severity and further limited access to traditional face-to-face CBT treatment due to health and safety precautions.

Purpose: This systematic review aims to demonstrate the efficacy of technology-based CBT by evaluating results of clinical trials and its comparability to traditional CBT methods.

Methods: An evidence-based search was conducted using terms such as “Obsessive-Compulsive disorder” AND “ internet” AND “cognitive behavior therapy” and “children”. A total of 716 articles were identified.

Results: After screening titles, abstracts, and full articles for relevance, 7 studies with a total of 254 subjects and four different programs involving technology-based CBT were included in this systematic review. Information gathered in this review support the use of technology-based CBT as an effective treatment in reducing the severity of OCD symptoms as shown by statistically significant reductions in Children Yale-Brown Obsessive-Compulsive Scale scores. Additionally, the results from this review support previous clinical studies demonstrating that effects of technology-based CBT were non-inferior to traditional CBT methods.

Conclusion: The results of this systematic review support the use of technology-based CBT for the treatment of OCD in children and adolescents, especially in times where access to in-person therapist sessions are not possible due to geographical or global concerns. Additional research is needed to understand the impact and acceptability of new CBT methods on daily-life as well as the effectiveness of technology-based methods on more severe cases of OCD.
Use of the Codon Table to Quantify the Evolutionary Role of Random Mutations
Authors: Mezei Mihaly
Published: September 2021

The various biases affecting RNA mutations during evolution is the subject of intense research, leaving the extent of the role of random mutations undefined. To remedy this lacuna, using the codon table, the number of codons representing each amino acid was correlated with the amino acid frequencies in different branches of the evolutionary tree. The correlations were seen to increase as evolution progressed. Furthermore, the number of RNA mutations that resulted in a given amino acid mutation were found to be correlated with several widely used amino acid similarity tables (used in sequence alignments). These correlations were seen to increase when the observed codon usage was factored in.
Long-Term EEG Component Analysis Method Based on Lasso Regression
Authors: Bo Hongjian , Li Haifeng , Wu Boying , Li Hongwei , Ma Lin
Published: September 2021

At present, there are very few analysis methods for long-term electroencephalogram (EEG) components. Temporal information is always ignored by most of the existing techniques in cognitive studies. Therefore, a new analysis method based on time-varying characteristics was proposed. First of all, a regression model based on Lasso was proposed to reveal the difference between acoustics and physiology. Then, Permutation Tests and Gaussian fitting were applied to find the highest correlation. A cognitive experiment based on 93 emotional sounds was designed, and the EEG data of 10 volunteers were collected to verify the model. The 48-dimensional acoustic features and 428 EEG components were extracted and analyzed together. Through this method, the relationship between the EEG components and the acoustic features could be measured. Moreover, according to the temporal relations, an optimal offset of acoustic features was found, which could obtain better alignment with EEG features. After the regression analysis, the significant EEG components were found, which were in good agreement with cognitive laws. This provides a new idea for long-term EEG components, which could be applied in other correlative subjects.
How Neurons in Deep Models Relate with Neurons in the Brain
Authors: Pavone Arianna , Plebe Alessio
Published: September 2021

In dealing with the algorithmic aspects of intelligent systems, the analogy with the biological brain has always been attractive, and has often had a dual function. On the one hand, it has been an effective source of inspiration for their design, while, on the other hand, it has been used as the justification for their success, especially in the case of Deep Learning (DL) models. However, in recent years, inspiration from the brain has lost its grip on its first role, yet it continues to be proposed in its second role, although we believe it is also becoming less and less defensible. Outside the chorus, there are theoretical proposals that instead identify important demarcation lines between DL and human cognition, to the point of being even incommensurable. In this article we argue that, paradoxically, the partial indifference of the developers of deep neural models to the functioning of biological neurons is one of the reasons for their success, having promoted a pragmatically opportunistic attitude. We believe that it is even possible to glimpse a biological analogy of a different kind, in that the essentially heuristic way of proceeding in modern DL development bears intriguing similarities to natural evolution.
Neuroevolutionary Control for Autonomous Soaring
Authors: Kim Eric J. , Perez Ruben E.
Published: September 2021

The energy efficiency and flight endurance of small unmanned aerial vehicles (SUAVs) can be improved through the implementation of autonomous soaring strategies. Biologically inspired flight techniques such as dynamic and thermal soaring offer significant energy savings through the exploitation of naturally occurring wind phenomena for thrustless flight. Recent interest in the application of artificial intelligence algorithms for autonomous soaring has been motivated by the pursuit of instilling generalized behavior in control systems, centered around the use of neural networks. However, the topology of such networks is usually predetermined, restricting the search space of potential solutions, while often resulting in complex neural networks that can pose implementation challenges for the limited hardware onboard small-scale autonomous vehicles. In exploring a novel method of generating neurocontrollers, this paper presents a neural network-based soaring strategy to extend flight times and advance the potential operational capability of SUAVs. In this study, the Neuroevolution of Augmenting Topologies (NEAT) algorithm is used to train efficient and effective neurocontrollers that can control a simulated aircraft along sustained dynamic and thermal soaring trajectories. The proposed approach evolves interpretable neural networks in a way that preserves simplicity while maximizing performance without requiring extensive training datasets. As a result, the combined trajectory planning and aircraft control strategy is suitable for real-time implementation on SUAV platforms.
The Effect of Feeding Hens a Peanut Skin-Containing Diet on Hen Performance, and Shell Egg Quality and Lipid Chemistry
Authors: Toomer Ondulla , Vu Thien , Wysocky Rebecca , Moraes Vera , Malheiros Ramon , Anderson Kenneth
Published: September 2021

Peanut skins are a considerable waste product with little current economic value or use. We aimed to determine the dietary effects of peanut skins on layer production performance and egg quality and chemistry of the eggs produced. Two hundred commercial hens were randomly assigned to four treatments (five replicates) and fed ad libitum for 8 weeks: conventional control diet, diet containing 24% high-oleic peanut (HOPN), diet containing 3% peanut skin (PN Skin), and a diet with 2.5% oleic acid (OA). Hens fed the HOPN diet had significantly reduced body weights relative to the control and PN Skin treatments, producing fewer total eggs over the 8-week experimental period. Eggs weights were similar between the control and PN Skin treatments at weeks 2 and 4, while eggs from the PN Skin treatment group were heavier than other treatments at weeks 6 and 8 of the experiment. Eggs produced from the HOPN treatment had reduced saturated fatty acid (FA) content in comparison to the other treatment groups, while similar between PN Skin and control eggs at week 8 of the experiment. This study suggests that PN skins may be a suitable alternative layer feed ingredient.
Continuous Practice of Conservation Agriculture for 3–5 Years in Intensive Rice-Based Cropping Patterns Reduces Soil Weed Seedbank
Authors: Hossain Mohammad Mobarak , Begum Mahfuza , Hashem Abul , Rahman Md. Moshiur , Haque Md. Enamul , Bell Richard W.
Published: September 2021

When farmers first shift from conventional tillage (CT) to conservation agriculture (CA) practices, the control of weeds may be more difficult, due to the absence of tillage. However, continuous CA, over several years, may alter the weed seedbank. The nature of the weed seedbank changes over time, in intensively cropped rice-based rotations that are typical of the Eastern Gangetic Plain, are not well understood. Two on-farm CA experiments were sampled (in Beluapara after 3 years and Digram after 5 years) in Bangladesh for the effects of strip planting (SP) and bed planting (BP) at both the sites, plus no-tillage (NT) in Beluapara, and increased retention of the residue of previous crops (20% vs. 50%). The conventional tillage (CT) and 20% residue was the control treatment. The weed seedbank in 0–15 cm soil was quantified by assessing the emergence of weeds from soils collected from the field after irrigation, (Boro) rice in Digram and wheat in Beluapara, and they were allowed to emerge in trays in a shade-house experiment. The year-round count of emerged weeds at both the locations revealed the fewest number of weed species (especially broadleaf weeds), and the lowest weed density and biomass in SP, followed by CT, BP, and NT, with 50% crop residue mulch. Relative to CT, the SP, BP, and NT produced relatively more perennials weeds, as follows: Alternanthera denticulata ((R.) Brown.), Cyperus rotundus (L.), Dentella repens (L.), Jussia deccurence (Walt.), Leersia hexandra (L.), and Solanum torvum (Sw.), which was the opposite of CT that was enriched with the following annual weeds: Cyperus iria (L.), Digitaria sanguinalis (L.), Euphorbia parviflora (L.), Fimbristylis miliacea (L.), Lindernia antipoda (L.), L. hyssopifolia (L.), and Monochoria hastata (L.). The soil weed seed bank reduced by 13% in SP, while it increased by 19% and 76% in BP and NT, respectively, compared with CT. The species diversity reduced in SP and NT, by 24% and 11%, respectively, but increased by 2% in BP. In 50% residue, the soil weed seed bank and species diversity reduced by 16% and 14%, respectively, relative to that of 20% residue. The continuous practice of CA, for 3 or more years, in two rice-based crop rotations, decreased the size of the weed seedbank, but increased the relative proliferation of specific perennial weeds.
Determination and Metabolite Profiling of Mixtures of Triterpenoid Saponins from Seeds of Chilean Quinoa (Chenopodium quinoa) Germplasm
Authors: Pandya Archis , Thiele Björn , Zurita-Silva Andres , Usadel Björn , Fiorani Fabio
Published: September 2021

The seed pericarp of Chenopodium quinoa Willd. (quinoa) contains a mixture of triterpenoid saponins conferring undesired organoleptic properties. In this study, we evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions. Relative saponin content ranged from 0.22 to 15.04 mg/g of seed dry weight among the genotypes studied and the genotype effect was significant (p < 0.001). About 75% of the genotypes could be classified as low-saponin content lines which is promising in view of ongoing plant breeding efforts. In addition to the quantitative determination of saponins, metabolic profiling was performed by LC-FTICR-MS and LC-MS/MS. We obtained highly accurate mass estimation from ion spectra allowing the identification of twelve saponins of the oleanane type. These differ in their aglycons and in the number and type of glycoside units. Interestingly, we identified a saponin compound that, to our knowledge, had not been reported previously. Our study highlights that there is considerable variability concerning saponin content in quinoa, which contributes to the valorization of genetic resources towards the identification of genotypes that could be utilized in current and future quinoa breeding programs.
Critical Evaluation of Two Commercial Biocontrol Agents for Their Efficacy against B. cinerea under In Vitro and In Vivo Conditions in Relation to Different Abiotic Factors
Authors: Tut Gurkan , Magan Naresh , Brain Philp , Xu Xiangming
Published: September 2021

The study evaluated the dose–response relationship of two commercial microbial biocontrol agents, Bacillus subtilis and Gliocladium catenulatum, against Botrytis cinerea both in vitro and in vivo. Inoculum doses, formulation, temperature and foliar leaf part all affected the control achieved by the two BCAs. In vitro competition assays on modified PDA plates tested a range of BCA doses (log10 3–10 CFUs or spores/droplet) at 4, 10 and 20 °C on the development of B. cinerea colonies. The dose–response relationship was influenced by both the BCA formulation and temperature. In vivo studies on lettuce plants in semi-commercial greenhouses examined the BCA dose (log10 5–9 CFUs or spores/mL) for controlling B. cinerea with a high inoculum (log10 6 spores/mL). Leaf disc assays showed that the dose–response relationship was influenced by the leaf parts sampled. These results suggest that the dose–response relationship between a BCA and specific pathogen will be significantly influenced by environmental conditions, formulation and plant phyllosplane tissue.
Management of Green Waste Streams from Different Origins: Assessment of Different Composting Scenarios
Authors: Guilabert Francisco J. , Barber Xavier , Pérez-Murcia María Dolores , Agulló Enrique , Andreu-Rodríguez Francisco Javier , Moral Raúl , Bustamante María Ángeles
Published: September 2021

The organic wastes of plant origin and, in particular, those coming from sources related to tourism activities, such as those generated from golf courses and touristic coasts, constitute an increasing concern due to the rise in their production and their unsuitable management. Thus, this work aimed to assess the use of different composting strategies to manage these specific green wastes, such as grass clippings and pruning waste from a golf course and marine plant debris, mainly from posidonia (Posidonia oceanica L.). To this end, two composting scenarios were established: the first only considered green wastes in the composition of the composting mixtures, and the second used sewage sludge as a co-composting agent. The temperature of the piles was monitored, and physicochemical and chemical parameters were also studied throughout the process. The results obtained showed that composting is a feasible method to manage and recycle this type of green waste, obtaining end products with suitable physicochemical and chemical characteristics. However, proportions of sea plant wastes in the composting mixture higher than 30% can compromise the fertilizing value of the final compost. Moreover, the use of an additional co-composting agent (sewage sludge) improved the characteristics of the end products obtained, provided that this co-composting agent had suitable initial characteristics.
Heat Waves and Broomrape Are the Major Constraints for Lentil Cultivation in Southern Spain
Authors: Rubiales Diego , Moral Ana , Flores Fernando
Published: September 2021

There is potential for expanding lentil cultivation to dry and warm Mediterranean rain-fed environments at low altitudes, where early sowings are recommended to profit from winter rains and escape drought and excessive heat at the grain filling stage. In cooler areas, frost might be a problem in the early sowings, however, in warmer areas such as our low altitude warm southern Spanish environments the most detrimental factor on lentil seed yield appeared to be high temperatures at grain-filling stage, particularly heat waves of more than 5 days with Tmax > 30 °C. This was followed by broomrape infection, the combination of both being dramatic. We detected variation for stress tolerance, with S17 and R7 accessions outstanding for all stress indexes used, followed by S23, Nsir, S6, and S12. Broomrape infection ranked second risk in the area. No complete resistance to broomrape was identified, but there was a significant variation in the level of infection, with accessions S14 and R17 being the more resistant across environments. This offers prospects for combining heat tolerance and broomrape resistance by breeding.
Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry
Authors: Lima Juliana França , Dias Maria Inês , Pereira Carla , Ivanov Marija , Soković Marina , Steinmacher Nádia Cristiane , Ferreira Isabel C. F. R. , Barros Lillian
Published: September 2021

Consumers have given preferences to food products that, in addition to the nutritional properties, also present bioactive characteristics with beneficial health effects. The use of Nonconventional Food Plants (NCFP) has been an asset for the food industry, not only due to its abundance but, also, because it does not compete with other vegetable matrices used for human consumption for its nutritional properties, chemical and bioactive potentiality. The present work aimed to study the granulometry and water absorption index of three seeds of unconventional food plants: niger, millet and birdseed, followed by their nutritional value, content in free sugars, fatty acids, organic acids, tocopherols and phenolic compounds by chromatographic methods and, also, the evaluation of the antioxidant, hepatotoxic and antimicrobial potential in their hydroethanolic extracts. Bakery products were developed with a partial replacement of wheat flour, using the centroid simplex method to understand the effect of applying NCFP flours in the final physical–chemical characteristics. The high granulometry associated with the high water absorption index indicates that the use of NCFP flours would have to be complemented with other flours for baking. The chemical composition of niger seed stood out the most; however, all seeds presented relatively low IC50 and MIC values for the inhibition of lipid peroxidation and antimicrobial activity, respectively. Finally, breads made with a mixture of millet and birdseed flour showed the greatest similarity to the control bread. Considering their composition in bioactive compounds, the use of these seeds is highly advisable in the context of a fortified diet, being sources of compounds of high nutritional value and with beneficial health effects for the final consumer.
Seed Priming with Sulfhydral Thiourea Enhances the Performance of Camelina sativa L. under Heat Stress Conditions
Authors: Waraich Ejaz Ahmad , Ahmad Muhammad , Soufan Walid , Manzoor Muhammad Taimoor , Ahmad Zahoor , Habib-Ur-Rahman Muhammad , Sabagh Ayman EL
Published: September 2021

Temperature is a key factor influencing plant growth and productivity; however, temperature fluctuations can cause detrimental effects on crop growth. This study aimed to assess the effect of seed priming on Camelina sativa L. under heat stress. Experimental treatments were comprised of; seed priming including, no-priming, hydropriming (distilled water priming), and osmopriming (thiourea applications at 500 ppm), heat stress (control = 20 °C and heat stress = 32 °C), and camelina varieties (7126 and 8046). Heat stress hammered crop growth as relative water content and photosynthetic rate were reduced by 35.9% and 49.05% in 7126, respectively, and 25.6% and 41.2% in 8046 as compared with control-no thiourea applied. However, osmopriming with thiourea improved the root and shoot length, and biomass production compared to control–no application under heat stress, with more improvement in variety 8046 as compared with 7126. Moreover, the maximum values of gas exchange and water relations were recorded at thiourea priming and no stress as compared with no-priming under heat stress that helped to improve seed yield by 12% in 7126 and 15% in 8046, respectively. Among the varieties, camelina variety 8046 showed better performance than 7126 by producing higher seed yield especially when subjected to thiourea priming. In conclusion, thiourea seed priming helped the plants to mitigate the adverse effects of heat stress by upregulating plant physiological attributes that lead to maintain camelina seed yield.
Synthesis and Characterization of Nano Fe and Mn (hydr)oxides to Be Used as Natural Sorbents and Micronutrient Fertilizers
Authors: Cieschi María Teresa , de Francisco Marina , Herrero Paula , Sánchez-Marcos Jorge , Cuevas Jaime , Esteban Elvira , Lucena Juan José , Yunta Felipe
Published: September 2021

Fe and Mn (hydr)oxides are widely used as contaminant sorbents in water/wastewater systems but their potential use as micronutrient fertilizers is still poorly known. In this research, four nano-metal (hydr)oxides (amorphous Mn oxide (AMO), Fe-Mn binary oxide (FMBO), two-line ferrihydrite (2L-Fh) and goethite) were successfully synthesized and completely characterized (infrared and Mössbauer spectroscopy, X-ray diffraction particle size, specific surface area, point of zero charge). AMO, FMBO and 2L-Fh were introduced to interact with AgNO3 (20.0 µM) and TlNO3 (100.0 µM) diluted solutions for three days to check their potential capability as potential Ag+ and Tl+ adsorbents. AMO and FMBO (4% w/w) were tested as nanofertilizers by arranging a hydroponic bioassay for 35 days on white lupin culture as a Mn-hyperaccumulator plant model. AMO structure was identified as an amorphous mixture of Mn oxides while FMBO was an Fe dopped birnessite. Both materials were efficient in extracting Ag+ and Tl+ although large Mn concentration was released from FMBO to the solutions. AMO and FMBO promoted Fe and Mn nutrition in plants. Synthetic iron chelate (Fe-EDDHA), present in the nutrient dissolution, could be adsorbed onto AMO surface by producing Fe and Mn accumulation in roots and increasing Mn uptake rate without toxicity symptoms. Therefore, AMO and FMBO not only demonstrated their efficiency as adsorbents, but also displayed they would be promising nanomaterials as micronutrient fertilizers.
Demodex crocidurae, a New Demodecid Mite (Acariformes: Prostigmata) Parasitizing the Lesser White-Toothed Shrew and a Redescription of Demodex talpae from European Mole with Data on Parasitism in Soricomorpha
Authors: Cierocka Karolina , Izdebska Joanna N. , Rolbiecki Leszek
Published: September 2021

Only six parasitic species of Demodecidae mite have thus far been described from the Soricomorpha, these being associated with the common shrew Sorex araneus Linnaeus, 1758, and the Mediterranean water shrew Neomys anomalus Cabrera, 1907 (two species from each host), and with the lesser white-toothed shrew Crocidura suaveolens (Pallas, 1811) and the European mole Talpa europaea Linnaeus, 1758 (one from each host species). Presently, Demodex crocidurae, a new species, has been described from the territory of Poland for C. suaveolens; in order to confirm its validity, it was necessary to redescribe D. talpae Hirst, 1921, from T. europaea, a demodecid species first described by Hirst in 1921 from England and then noted only in Poland. Both species colonized the hairy skin of the body in their hosts, where no disease symptoms of infestation were observed. However, D. crocidurae showed higher infection parameters (prevalence 100%, mean intensity 11.7, intensity range 3–26 individuals) than those of D. talpae (30.0%, 4.7, 2.0–8.0), possibly due to different host biology.
Enterocytes in Food Hypersensitivity Reactions
Authors: Krstić Ristivojević Maja , Apostolović Danijela , Smiljanić Katarina
Published: September 2021

Food hypersensitivity reactions are adverse reactions to harmless dietary substances, whose causes are hidden within derangements of the complex immune machinery of humans and mammals. Until recently, enterocytes were considered as solely absorptive cells providing a physical barrier for unwanted lumen constituents. This review focuses on the enterocytes, which are the hub for innate and adaptive immune reactions. Furthermore, the ambiguous nature of enterocytes is also reflected in the fact that enterocytes can be considered as antigen-presenting cells since they constitutively express major histocompatibility complex (MHC) class II molecules. Taken together, it becomes clear that enterocytes have an immense role in maintaining oral tolerance to foreign antigens. In general, the immune system and its mechanisms underlying food hypersensitivity are still unknown and the involvement of components belonging to other anatomical systems, such as enterocytes, in these mechanisms make their elucidation even more difficult. The findings from studies with animal models provide us with valuable information about allergic mechanisms in the animal world, while on the other hand, these models are used to extrapolate results to the pathological conditions occurring in humans. There is a constant need for studies that deal with this topic and can overcome the glitches related to ethics in working with animals.
Branched-Chain Amino Acid Supplementation Alters the Abundance of Mechanistic Target of Rapamycin and Insulin Signaling Proteins in Subcutaneous Adipose Explants from Lactating Holstein Cows
Authors: Liang Yusheng , Cardoso Fabiana F. , Parys Claudia , Cardoso Felipe C. , Loor Juan J.
Published: September 2021

The objective of this study was to investigate changes in protein abundance of mTOR and insulin signaling pathway components along with amino acid (AA) transporters in bovine s.c. adipose (SAT) explants in response to increased supply of Leu, Ile, or Val. Explants of SAT from four lactating Holstein cows were incubated with high-glucose serum-free DMEM, to which the 10 essential AAs were added to create the following treatments: ideal mix of essential AA (IPAA; Lys:Met 2.9:1; Lys:Thr 1.8:1; Lys:His 2.38:1; Lys:Val 1.23:1; Lys:Ile 1.45:1; Lys:Leu 0.85:1; Lys:Arg 2.08:1) or IPAA supplemented with Ile, Val, or Leu to achieve a Lys:Ile of 1.29:1 (incIle), Lys:Val 1.12:1 (incVal), or Lys:Leu (incLeu) 0.78:1 for 4 h. Compared with IPAA, incLeu or incIle led to greater activation of protein kinase B (AKT; p-AKT/total AKT) and mTOR (p-mTOR/total mTOR). Total EAA in media averaged 7.8 ± 0.06 mmol/L across treatments. Incubation with incLeu, incIle, or incVal led to greater protein abundance of solute carrier family 38 member 1 (SLC38A1), a Gln transporter, and the BCAA catabolism enzyme branched-chain α-keto acid dehydrogenase kinase (BCKDK) compared with IPAA. Activation of eukaryotic elongation factor 2 (eEF2; p-eEF2/total eEF2) was also greater in response to incLeu, incIle, or incVal. Furthermore, compared with incLeu or incIle, incVal supplementation led to greater abundance of SLC38A1 and BCKDK. BCKDK is a rate-limiting enzyme regulating BCAA catabolism via inactivation and phosphorylation of the BCKD complex. Overall, data suggested that enhanced individual supplementation of BCAA activates mTOR and insulin signaling in SAT. Increased AA transport into tissue and lower BCAA catabolism could be part of the mechanism driving these responses. The potential practical applications for enhancing post-ruminal supply of BCAA via feeding in rumen-protected form support in vivo studies to ascertain the role of these AAs on adipose tissue biology.
Partial and Total Replacement of Soybean Meal with Full-Fat Black Soldier Fly (Hermetia illucens L.) Larvae Meal in Broiler Chicken Diets: Impact on Growth Performance, Carcass Quality and Meat Quality
Authors: Murawska Daria , Daszkiewicz Tomasz , Sobotka Wiesław , Gesek Michał , Witkowska Dorota , Matusevičius Paulius , Bakuła Tadeusz
Published: September 2021

The aim of this study was to compare selected growth performance parameters and slaughter characteristics in broiler chickens fed diets with a different content of full-fat Hermetia illucens L. (HI) larvae meal. The experiment was performed on 384 male broiler chickens (Ross 308) reared to 42 d of age and assigned to four dietary treatments (HI0—control diet and diets where soybean meal protein (SBM) was replaced with HI protein in 50%, 75% and 100%, respectively). The final body weights of chickens were as follows: 3010.0 g (HI0), 2650.0 g (HI50), 2590.0 g (HI75) and 2375.0 g (H100, p < 0.05). The carcasses of chickens from the experimental groups contained less meat and more abdominal fat. The feed conversion ratio for the entire experimental period was similar in groups HI0, HI50 and HI75 and more desirable than in group HI100 (p < 0.05). The meat of broiler chickens from groups HI75 and HI100 was characterized by significantly (p < 0.05) lower juiciness and taste intensity than the meat of birds from groups HI0 and HI50. The replacement of SBM protein with full-fat HI larvae meal in broiler diets exceeding 50% significantly compromised the growth performance of birds and the carcass and meat quality.
Effect of Linseeds and Hemp Seeds on Milk Production, Energy and Nitrogen Balance, and Methane Emissions in the Dairy Goat
Authors: Rapetti Luca , Colombini Stefania , Battelli Giovanna , Castiglioni Bianca , Turri Federica , Galassi Gianluca , Battelli Marco , Crovetto Gianni Matteo
Published: September 2021

The effect of whole linseeds or hemp seeds on milk production, energy and nitrogen balance, and methane emission was studied in 12 Alpine goats using respiration chambers. Diets tested were a control diet (C) and two diets supplemented with whole linseeds (L) or hemp seeds (H) at 9.3% on a dry matter (DM) basis. DM intake was similar among treatments, whereas DM and organic matter digestibility were lower for L compared to C. Milk yield (2.30 kg/d on average) and rumen fermentation profile were not affected by treatments. Treatment also did not affect the milk composition, with the exception of fat, which was higher in H and L compared to C (4.21, 3.94, and 3.20%, respectively). Oilseed supplementation caused a reduction in the concentration of de novo fatty acids (FA) (41.1, 48.8, and 64.1% of FA, for L, H, and C, respectively). Moreover, L and H diets reduced the sum of saturated FA, and increased monounsaturated FA, whereas only the L diet increased the concentration of polyunsaturated FA. Regarding methane production, and nitrogen and energy balances, no differences were registered among the diets. Our research indicates that including whole linseeds and hemp seeds in the dairy goat diet is an effective strategy for increasing milk fat content and positively modifying the milk FA composition, without a change in nitrogen and energy balances, but also without a reduction in enteric methane emission.
Tracking Devices for Pets: Health Risk Assessment for Exposure to Radiofrequency Electromagnetic Fields
Authors: Klune Judith , Arhant Christine , Windschnurer Ines , Heizmann Veronika , Schauberger Günther
Published: September 2021

Every year, approximately 3% of cats and dogs are lost. In addition to passive methods for identifying pets, radiofrequency tracking devices (TDs) are available. These TDs can track a pet’s geographic position, which is transmitted by radio frequencies. The health risk to the animals from continuous exposure to radiofrequency electromagnetic fields (RF-EMFs) was reviewed. Fourteen out of twenty-one commercially available TDs use 2G, 3G, or 4G mobile networks, and the others work with public frequencies, WLAN, Bluetooth, etc. The exposure of pets to RF-EMFs was assessed, including ambient exposure (radios, TVs, and base stations of mobile networks), exposure from indoor devices (DECT, WLAN, Bluetooth, etc.), and the exposure from TDs. The exposure levels of the three areas were found to be distinctly below the International Commission on Non-Ionising Radiation Protection (ICNIRP) reference levels, which assure far-reaching protection from adverse health effects. The highest uncertainty regarding the exposure of pets was related to that caused by indoor RF-emitting devices using WLAN and DECT. This exposure can be limited considerably through a reduction in the exposure time and an increase in the distance between the animal and the RF-emitting device. Even though the total RF-EMF exposure level experienced by pets was found to be below the reference limits, recommendations were derived to reduce potential risks from exposure to TDs and indoor devices.
Procalcitonin to Guide Antibacterial Prescribing in Patients Hospitalised with COVID-19
Authors: Hughes Stephen , Mughal Nabeela , Moore Luke S. P.
Published: September 2021

Antibacterial prescribing in patients presenting with COVID-19 remains discordant to rates of bacterial co-infection. Implementing diagnostic tests to exclude bacterial infection may aid reduction in antibacterial prescribing. (1) Method: A retrospective observational analysis was undertaken of all hospitalised patients with COVID-19 across a single-site NHS acute Trust (London, UK) from 1 December 2020 to 28 February 2021. Electronic patient records were used to identify patients, clinical data, and outcomes. Procalcitonin (PCT) serum assays, where available on admission, were analysed against electronic prescribing records for antibacterial prescribing to determine relationships with a negative PCT result (<25 mg/L) and antibacterial course length. (2) Results: Antibacterial agents were initiated on admission in 310/624 (49.7%) of patients presenting with COVID-19. A total of 33/74 (44.5%) patients with a negative PCT on admission had their treatment stopped within 24 h. A total of 6/49 (12.2%) patients were started on antibacterials, but a positive PCT saw their treatment stopped. Microbiologically confirmed bacterial infection was low (19/594; 3.2%) and no correlation was seen between PCT and culture positivity (p = 1). Lower mortality (15.6% vs. 31.4%; p = 0.049), length of hospital stay (7.9 days vs. 10.1 days; p = 0.044), and intensive care unit (ICU) admission (13.9% vs. 40.8%; p = 0.001) was noted among patients with low PCT. (3) Conclusions: This retrospective analysis of community acquired COVID-19 patients demonstrates the potential role of PCT in excluding bacterial co-infection. A negative PCT on admission correlates with shorter antimicrobial courses, early cessation of therapy, and predicts lower frequency of ICU admission. Low PCT may support decision making in cessation of antibacterials at the 48–72 h review.
Local Trends of Antibiotic Prescriptions for Necrotizing Fasciitis Patients in Two Tertiary Care Hospitals in Central Malaysia
Authors: Rampal Sanjiv , Ganesan Thanusha , Sisubalasingam Narresh , Neela Vasantha Kumari , Tokgöz Mehmet Ali , Arunasalam Arun , Ab Halim Mohd Asyraf Hafizuddin , Shamsudin Zulfahrizzat Bin , Kumar Suresh , Sinniah Ajantha
Published: September 2021

Background: Necrotizing fasciitis (NF) is a rapidly progressive inflammatory infection of the soft tissue (also known as the fascia) with a secondary necrosis of the subcutaneous tissues, leading to a systemic inflammatory response syndrome (SIRS), shock and eventually death despite the availability of current medical interventions. The clinical management of this condition is associated with a significant amount of morbidity with a high rate of mortality. The prognosis of the disease is affected by multiple factors, which include the virulence of the causative pathogen, local host immunity, local wound factors and empirical antibiotics used. The local trends in the prescription of empirical antibiotics are often based on clinical practice guidelines (CPG), the distribution of the causative microorganism and the cost-effectiveness of the drug. However, there appears to be a paucity of literature on the empirical antibiotic of choice when dealing with necrotizing fasciitis in the clinical setting. This paper will outline common causative microorganisms and current trends of prescription in two tertiary centres in Central Malaysia. Methods: This was a cross-sectional study using retrospective data of patients treated for NF collected from two tertiary care hospitals (Hospital Seremban and Hospital Ampang) in Central Malaysia. A total of 420 NF patients were identified from the five years of retrospective data obtained from the two hospitals. Results: The top three empirical antibiotics prescribed are ampicillin + sulbactam (n = 258; 61.4%), clindamycin (n = 55; 13.1%) and ceftazidime (n = 41; 9.8%). The selection of the antibiotic significantly impacts the outcome of NF. The top three causative pathogens for NF are Streptococcus spp. (n = 79; 18.8%), Pseudomonas aeruginosa (n = 61; 14.5%) and Staphylococcus spp. (n = 49; 11.7%). The patients who received antibiotics had 0.779 times lower chances of being amputated. Patients with a lower laboratory risk indicator for necrotizing fasciitis (LRINEC) score had 0.934 times lower chances of being amputated. Conclusions: In this study, the most common empirical antibiotic prescribed was ampicillin + sulbactam followed by clindamycin and ceftazidime. The antibiotics prescribed lower the risk of having an amputation and, hence, a better prognosis of the disease. Broad-spectrum empirical antibiotics following surgical debridement reduce the mortality rate of NF.
Genomic Investigation of Methicillin-Resistant Staphylococcus aureus ST113 Strains Isolated from Tertiary Care Hospitals in Pakistan
Authors: Ullah Nimat , Dar Hamza Arshad , Naz Kanwal , Andleeb Saadia , Rahman Abdur , Saeed Muhammad Tariq , Hanan Fazal , Bae Taeok , Ali Amjad
Published: September 2021

Methicillin-resistant Staphylococcus aureus (MRSA) is a multi-drug resistant and opportunistic pathogen. The emergence of new clones of MRSA in both healthcare settings and the community warrants serious attention and epidemiological surveillance. However, epidemiological data of MRSA isolates from Pakistan are limited. We performed a whole-genome-based comparative analysis of two (P10 and R46) MRSA strains isolated from two provinces of Pakistan to understand the genetic diversity, sequence type (ST), and distribution of virulence and antibiotic-resistance genes. The strains belong to ST113 and harbor the SCCmec type IV encoding mecA gene. Both the strains contain two plasmids, and three and two complete prophage sequences are present in P10 and R46, respectively. The specific antibiotic resistance determinants in P10 include two aminoglycoside-resistance genes, aph(3’)-IIIa and aad(6), a streptothrin-resistance gene sat-4, a tetracycline-resistance gene tet(K), a mupirocin-resistance gene mupA, a point mutation in fusA conferring resistance to fusidic acid, and in strain R46 a specific plasmid associated gene ant(4’)-Ib. The strains harbor many virulence factors common to MRSA. However, no Panton-Valentine leucocidin (lukF-PV/lukS-PV) or toxic shock syndrome toxin (tsst) genes were detected in any of the genomes. The phylogenetic relationship of P10 and R46 with other prevailing MRSA strains suggests that ST113 strains are closely related to ST8 strains and ST113 strains are a single-locus variant of ST8. These findings provide important information concerning the emerging MRSA clone ST113 in Pakistan and the sequenced strains can be used as reference strains for the comparative genomic analysis of other MRSA strains in Pakistan and ST113 strains globally.
Antibiotic Prescribing Patterns in Ghana, Uganda, Zambia and Tanzania Hospitals: Results from the Global Point Prevalence Survey (G-PPS) on Antimicrobial Use and Stewardship Interventions Implemented
Authors: D’Arcy Nikki , Ashiru-Oredope Diane , Olaoye Omotayo , Afriyie Daniel , Akello Zainab , Ankrah Daniel , Asima Derrick Mawuena , Banda David C. , Barrett Scott , Brandish Claire , Brayson Joseph , Benedict Peter , Dodoo Cornelius C. , Garraghan Frances , Hoyelah Josephyn , Jani Yogini , Kitutu Freddy Eric , Kizito Ismail Musoke , Labi Appiah-Korang , Mirfenderesky Mariyam , Murdan Sudaxshina , Murray Caoimhe , Obeng-Nkrumah Noah , Olum William J’Pathim , Opintan Japheth Awuletey , Panford-Quainoo Edwin , Pauwels Ines , Sefah Israel , Sneddon Jacqueline , St. Clair Jones Anja , Versporten Ann
Published: September 2021

Antimicrobial resistance (AMR) remains an important global public health issue with antimicrobial misuse and overuse being one of the main drivers. The Global Point Prevalence Survey (G-PPS) of Antimicrobial Consumption and Resistance assesses the prevalence and the quality of antimicrobial prescriptions across hospitals globally. G-PPS was carried out at 17 hospitals across Ghana, Uganda, Zambia and Tanzania. The overall prevalence of antimicrobial use was 50% (30–57%), with most antibiotics prescribed belonging to the WHO ‘Access’ and ‘Watch’ categories. No ‘Reserve’ category of antibiotics was prescribed across the study sites while antimicrobials belonging to the ‘Not Recommended’ group were prescribed infrequently. Antimicrobials were most often prescribed for prophylaxis for obstetric or gynaecological surgery, making up between 12 and 18% of total prescriptions across all countries. The most prescribed therapeutic subgroup of antimicrobials was ‘Antibacterials for systemic use’. As a result of the programme, PPS data are now readily available for the first time in the hospitals, strengthening the global commitment to improved antimicrobial surveillance. Antimicrobial stewardship interventions developed included the formation of AMS committees, the provision of training and the preparation of new AMS guidelines. Other common interventions included the presentation of findings to clinicians for increased awareness, and the promotion of a multi-disciplinary approach to successful AMS programmes. Repeat PPS would be necessary to continually monitor the impact of interventions implemented. Broader participation is also encouraged to strengthen the evidence base.
Over-the-Counter Sale of Antibiotics in India: A Qualitative Study of Providers’ Perspectives across Two States
Authors: Kotwani Anita , Joshi Jyoti , Lamkang Anjana Sankhil
Published: September 2021

India has one of the highest rates of antimicrobial resistance (AMR) worldwide. Despite being prescription drugs, antibiotics are commonly available over-the-counter (OTC) at retail pharmacies. We aimed to gain insight into the OTC sale of antibiotics at retail pharmacies and to elucidate its underlying drivers. We conducted face-to-face, in-depth interviews using convenience sampling with 22 pharmacists and 14 informal dispensers from 36 retail pharmacies across two Indian states (Haryana and Telangana). Thematic analysis revealed that antibiotics were often dispensed OTC for conditions e.g., fever, cough and cold, and acute diarrhea, which are typically viral and self-limiting. Both Access and Watch groups of antibiotics were dispensed for 1–2 days. Respondents had poor knowledge regarding AMR and shifted the blame for OTC practices for antibiotics onto the government, prescribers, informal providers, cross practice by alternative medicine practitioners, and consumer demand. Pharmacists suggested the main drivers for underlying OTC dispensing were commercial interests, poor access to public healthcare, economic and time constraints among consumers, lack of stringent regulations, and scanty inspections. Therefore, a comprehensive strategy which is well aligned with activities under the National Action Plan-AMR, including stewardship efforts targeting pharmacists and evidence-based targeted awareness campaigns for all stakeholders, is required to curb the inappropriate use of antibiotics.
Understanding Antibiotic Usage on Small-Scale Dairy Farms in the Indian States of Assam and Haryana Using a Mixed-Methods Approach—Outcomes and Challenges
Authors: Kumar Naresh , Sharma Garima , Leahy Eithne , Shome Bibek R. , Bandyopadhyay Samiran , Deka Ram Pratim , Shome Rajeswari , Dey Tushar Kumar , Lindahl Johanna Frida
Published: September 2021

The use and misuse of antibiotics in both humans and animals contributes to the global emergence of antimicrobial resistant (AMR) bacteria, a threat to public health and infection control. Currently, India is the world’s leading milk producer but antibiotic usage within the dairy sector is poorly regulated. Little data exists reflecting how antibiotics are used on dairy farms, especially on small-scale dairy farms in India. To address this lack of data, a study was carried out on 491 small-scale dairy farms in two Indian states, Assam and Haryana, using a mixed method approach where farmers were interviewed, farms inspected for the presence of antibiotics and milk samples taken to determine antibiotic usage. Usage of antibiotics on farms appeared low only 10% (95% CI 8–13%) of farmers surveyed confirmed using antibiotics in their dairy herds during the last 12 months. Of the farms surveyed, only 8% (6–11%) had milk samples positive for antibiotic residues, namely from the novobiocin, macrolides, and sulphonamide classes of antibiotics. Of the farmers surveyed, only 2% (0.8–3%) had heard of the term “withdrawal period” and 53% (40–65%) failed to describe the term “antibiotic”. While this study clearly highlights a lack of understanding of antibiotics among small-scale dairy farmers, a potential factor in the emergence of AMR bacteria, it also shows that antibiotic usage on these farms is low and that the possible role these farmers play in AMR emergence may be overestimated.
Genome-Wide Characterization of Glutathione Peroxidase (GPX) Gene Family in Rapeseed (Brassica napus L.) Revealed Their Role in Multiple Abiotic Stress Response and Hormone Signaling
Authors: Li Wei , Huai Xuemin , Li Peitao , Raza Ali , Mubarik Muhammad Salman , Habib Madiha , Fiaz Sajid , Zhang Binbin , Pan Jun , Khan Rao Sohail Ahmad
Published: September 2021

Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H2O2 homeostasis and normalize plant reaction to abiotic stress conditions. To understand the major roles of the GPX gene family in rapeseed (Brassica napus L.), for the first time, a genome-wide study identified 25 BnGPX genes in the rapeseed genome. The phylogenetic analysis discovered that GPX genes were grouped into four major groups (Group I–Group IV) from rapeseed and three closely interrelated plant species. The universal investigation uncovered that the BnGPXs gene experienced segmental duplications and positive selection pressure. Gene structure and motifs examination recommended that most of the BnGPX genes demonstrated a comparatively well-maintained exon-intron and motifs arrangement within the identical group. Likewise, we recognized five hormones-, four stress-, and numerous light-reactive cis-elements in the promoters of BnGPXs. Five putative bna-miRNAs from two families were also prophesied, targeting six BnGPXs genes. Gene ontology annotation results proved the main role of BnGPXs in antioxidant defense systems, ROS, and response to stress stimulus. Several BnGPXs genes revealed boosted expression profiles in many developmental tissues/organs, i.e., root, seed, leaf, stem, flower, and silique. The qRT-PCR based expression profiling exhibited that two genes (BnGPX21 and BnGPX23) were suggestively up-regulated against different hormones (ABA, IAA, and MeJA) and abiotic stress (salinity, cold, waterlogging, and drought) treatments. In short, our discoveries provide a basis for additional functional studies on the BnGPX genes in future rapeseed breeding programs.