bluuebunny/crossref_metadata_2025 huggingface dataset:
Bacterial enrichment prior to third-generation metagenomic sequencing improves detection of BRD pathogens and genetic determinants of antimicrobial resistance in feedlot cattle
Authors: Herman Emily K. , Lacoste Stacey R. , Freeman Claire N. , Otto Simon J. G. , McCarthy E. Luke , Links Matthew G. , Stothard Paul , Waldner Cheryl L.
Published: May 2024

Introduction

Bovine respiratory disease (BRD) is one of the most important animal health problems in the beef industry. While bacterial culture and antimicrobial susceptibility testing have been used for diagnostic testing, the common practice of examining one isolate per species does not fully reflect the bacterial population in the sample. In contrast, a recent study with metagenomic sequencing of nasal swabs from feedlot cattle is promising in terms of bacterial pathogen identification and detection of antimicrobial resistance genes (ARGs). However, the sensitivity of metagenomic sequencing was impeded by the high proportion of host biomass in the nasal swab samples.

Methods

This pilot study employed a non-selective bacterial enrichment step before nucleic acid extraction to increase the relative proportion of bacterial DNA for sequencing.

Results

Non-selective bacterial enrichment increased the proportion of bacteria relative to host sequence data, allowing increased detection of BRD pathogens compared with unenriched samples. This process also allowed for enhanced detection of ARGs with species-level resolution, including detection of ARGs for bacterial species of interest that were not targeted for culture and susceptibility testing. The long-read sequencing approach enabled ARG detection on individual bacterial reads without the need for assembly. Metagenomics following non-selective bacterial enrichment resulted in substantial agreement for four of six comparisons with culture for respiratory bacteria and substantial or better correlation with qPCR. Comparison between isolate susceptibility results and detection of ARGs was best for macrolide ARGs in Mannheimia haemolytica reads but was also substantial for sulfonamide ARGs within M. haemolytica and Pasteurella multocida reads and tetracycline ARGs in Histophilus somni reads.

Discussion

By increasing the proportion of bacterial DNA relative to host DNA through non-selective enrichment, we demonstrated a corresponding increase in the proportion of sequencing data identifying BRD-associated pathogens and ARGs in deep nasopharyngeal swabs from feedlot cattle using long-read metagenomic sequencing. This method shows promise as a detection strategy for BRD pathogens and ARGs and strikes a balance between processing time, input costs, and generation of on-target data. This approach could serve as a valuable tool to inform antimicrobial management for BRD and support antimicrobial stewardship.
Cardiovascular disease risk exacerbates brain aging among Hispanic/Latino adults in the SOL-INCA-MRI Study
Authors: Stickel Ariana M. , Tarraf Wassim , Gonzalez Kevin A. , Paredes Alejandra Morlett , Zeng Donglin , Cai Jianwen , Isasi Carmen R. , Kaplan Robert , Lipton Richard B. , Daviglus Martha L. , Testai Fernando D. , Lamar Melissa , Gallo Linda C. , Talavera Gregory A. , Gellman Marc D. , Ramos Alberto R. , Ivanovic Vladimir , Seiler Stephan , González Hector M. , DeCarli Charles
Published: May 2024

Background

Cardiovascular disease (CVD) risk factors are highly prevalent among Hispanic/Latino adults, while the prevalence of MRI infarcts is not well-documented. We, therefore, sought to examine the relationships between CVD risk factors and infarcts with brain structure among Hispanic/Latino individuals.

Methods

Participants included 1,886 Hispanic/Latino adults (50–85 years) who underwent magnetic resonance imaging (MRI) as part of the Study of Latinos—Investigation of Neurocognitive Aging-MRI (SOL-INCA-MRI) study. CVD risk was measured approximately 10.5 years before MRI using the Framingham cardiovascular risk score, a measure of 10-year CVD risk (low (<10%), medium (10- < 20%), and high (≥20%)). MR infarcts were determined as present or absent. Outcomes included total brain, cerebral and lobar cortical gray matter, hippocampal, lateral ventricle, and total white matter hyperintensity (WMH) volumes. Linear regression models tested associations between CVD risk and infarct with MRI outcomes and for modifications by age and sex.

Results

Sixty percent of participants were at medium or high CVD risk. Medium and high CVD risk were associated with lower total brain and frontal gray matter and higher WMH volumes compared to those with low CVD risk. High CVD risk was additionally associated with lower total cortical gray matter and parietal volumes and larger lateral ventricle volumes. Men tended to have greater CVDRF-related differences in total brain volumes than women. The association of CVD risk factors on total brain volumes increased with age, equal to an approximate 7-year increase in total brain aging among the high-CVD-risk group compared to the low-risk group. The presence of infarct(s) was associated with lower total brain volumes, which was equal to an approximate 5-year increase in brain aging compared to individuals without infarcts. Infarcts were also associated with smaller total cortical gray matter, frontal and parietal volumes, and larger lateral ventricle and WMH volumes.

Conclusion

The high prevalence of CVD risk among Hispanic/Latino adults may be associated with accelerated brain aging.
ECG-based estimation of respiration-induced autonomic modulation of AV nodal conduction during atrial fibrillation
Authors: Plappert Felix , Engström Gunnar , Platonov Pyotr G. , Wallman Mikael , Sandberg Frida
Published: May 2024

Introduction: Information about autonomic nervous system (ANS) activity may offer insights about atrial fibrillation (AF) progression and support personalized AF treatment but is not easily accessible from the ECG. In this study, we propose a new approach for ECG-based assessment of respiratory modulation in atrioventricular (AV) nodal refractory period and conduction delay.

Methods: A 1-dimensional convolutional neural network (1D-CNN) was trained to estimate respiratory modulation of AV nodal conduction properties from 1-minute segments of RR series, respiration signals, and atrial fibrillatory rates (AFR) using synthetic data that replicates clinical ECG-derived data. The synthetic data were generated using a network model of the AV node and 4 million unique model parameter sets. The 1D-CNN was then used to analyze respiratory modulation in clinical deep breathing test data of 28 patients in AF, where an ECG-derived respiration signal was extracted using a novel approach based on periodic component analysis.

Results: We demonstrated using synthetic data that the 1D-CNN can estimate the respiratory modulation from RR series alone with a Pearson sample correlation of r = 0.805 and that the addition of either respiration signal (r = 0.830), AFR (r = 0.837), or both (r = 0.855) improves the estimation.

Discussion: Initial results from analysis of ECG data suggest that our proposed estimate of respiration-induced autonomic modulation, aresp, is reproducible and sufficiently sensitive to monitor changes and detect individual differences. However, further studies are needed to verify the reproducibility, sensitivity, and clinical significance of aresp.
Overexpression of stress granule protein TZF1 enhances salt stress tolerance by targeting ACA11 mRNA for degradation in Arabidopsis
Authors: He Siou-Luan , Li Bin , Zahurancik Walter J. , Arthur Henry C. , Sidharthan Vaishnavi , Gopalan Venkat , Wang Lei , Jang Jyan-Chyun
Published: May 2024

Tandem CCCH zinc finger (TZF) proteins play diverse roles in plant growth and stress response. Although as many as 11 TZF proteins have been identified in Arabidopsis, little is known about the mechanism by which TZF proteins select and regulate the target mRNAs. Here, we report that Arabidopsis TZF1 is a bona-fide stress granule protein. Ectopic expression of TZF1 (TZF1 OE), but not an mRNA binding-defective mutant (TZF1H186Y OE), enhances salt stress tolerance in Arabidopsis. RNA-seq analyses of NaCl-treated plants revealed that the down-regulated genes in TZF1 OE plants are enriched for functions in salt and oxidative stress responses. Because many of these down-regulated mRNAs contain AU- and/or U-rich elements (AREs and/or UREs) in their 3’-UTRs, we hypothesized that TZF1—ARE/URE interaction might contribute to the observed gene expression changes. Results from RNA immunoprecipitation-quantitative PCR analysis, gel-shift, and mRNA half-life assays indicate that TZF1 binds and triggers degradation of the autoinhibited Ca2+-ATPase 11 (ACA11) mRNA, which encodes a tonoplast-localized calcium pump that extrudes calcium and dampens signal transduction pathways necessary for salt stress tolerance. Furthermore, this salt stress-tolerance phenotype was recapitulated in aca11 null mutants. Collectively, our findings demonstrate that TZF1 binds and initiates degradation of specific mRNAs to enhance salt stress tolerance.
Phyto-synthesized silver nanoparticles from Sargassum subrepandum: anticancer, antimicrobial, and molluscicidal activities
Authors: El-Sayed Heba , Abdelsalam Asmaa , Morad Mostafa Y. , Sonbol Hana , Ibrahim Amina M. , Tawfik Eman
Published: May 2024

In the realm of nanotechnology, the use of algae to produce nanoparticles is an environmentally friendly, sustainable, and economically viable strategy. In the present study, the brown macroalgae Sargassum subrepandum was utilized to effectively produce silver nanoparticles (AgNPs). Through various characterization techniques, the AgNPs’ structural integrity was confirmed. AgNPs exhibited significant antimicrobial activity against Pseudomonas aeruginosa and Fusarium equiseti. AgNPs showed cytotoxic effects on the MCF-7 breast adenocarcinoma cell line with an IC50 of 12.5 µg/ml. Treatment with AgNPs resulted in a marked reduction in cell viability, alongside evident apoptotic and necrotic morphological changes in the cancer cells. Through molecular docking studies, a deeper understanding of the interaction between AgNPs and crucial proteins related to cancer has been achieved, AgNPs showed a promising molluscicidal action on Biomphalaria alexandrina snails, a Schistosoma mansoni intermediate host. The half-lethal dose (LC50) of AgNPs was determined to be 0.84 mg/L. The potential consequences of its administration include potential disruptions to the glycolysis profile, as well as potential impacts on the steroidal hormone’s estrogen and testosterone and certain kidney function tests. This study highlights the diverse uses of algae-synthesized AgNPs, ranging from healthcare to environmental management, demonstrating their importance in advancing nano-biotechnological solutions.
Association among attention-deficit hyperactivity disorder, restless legs syndrome, and peripheral iron status: a two-sample Mendelian randomization study
Authors: Xiao Guoqiang , Shi Hongting , Lan Qiaoyu , Hu Jiajia , Guan Jincheng , Liang Zhuoji , Zhou Chumeng , Huang Zitong , Chen Yongyuan , Zhou Borong
Published: May 2024

Background

Epidemiological evidence indicates a high correlation and comorbidity between Attention Deficit Hyperactivity Disorder (ADHD) and Restless Legs Syndrome (RLS).

Objective

We aimed to investigate the causal relationship and shared genetic architecture between ADHD and RLS, as well as explore potential causal associations between both disorders and peripheral iron status.

Methods

We performed two-sample Mendelian randomization (MR) analyses using summary statistics from genome-wide meta-analyses of ADHD, RLS, and peripheral iron status (serum iron, ferritin, transferrin saturation, and total iron binding capacity). Additionally, we employed linkage disequilibrium score regression (LDSC) to assess genetic correlations between ADHD and RLS using genetic data.

Results

Our MR results supports a causal effect from ADHD (as exposure) to RLS (as outcome) (inverse variance weighted OR = 1.20, 95% CI: 1.08-1.34, p = 0.001). Conversely, we found no a causal association from RLS to ADHD (inverse variance weighted OR = 1.04, 95% CI: 0.99-1.09, p = 0.11). LDSC analysis did not detect a significant genetic correlation between RLS and ADHD (Rg = 0.3, SE = 0.16, p = 0.068). Furthermore, no evidence supported a causal relationship between peripheral iron deficiency and the RLS or ADHD onset. However, RLS may have been associated with a genetic predisposition to reduced serum ferritin levels (OR = 1.20, 95% CI: 1.00-1.04, p = 0.047).

Conclusion

This study suggests that ADHD is an independent risk factor for RLS, while RLS may confer a genetic predisposition to reduced serum ferritin levels.

Limitations

The GWAS summary data utilized originated from populations of European ancestry, limiting the generalizability of conclusions to other populations.

Clinical implications

The potential co-occurrence of RLS in individuals with ADHD should be considered during diagnosis and treatment. Moreover, iron supplementation may be beneficial for alleviating RLS symptoms.
Evaluating the effectiveness of a mobile app-based self-guided psychological interventions to reduce relapse in substance use disorder: protocol for a randomized controlled trial
Authors: Redeł Anna , Binkowska Alicja Anna , Obarska Katarzyna , Marcowski Przemysław , Szymczak Karol , Lewczuk Karol , Solich Katarzyna , Banaszak Maria , Woronowicz Bohdan , Nowicka Małgorzata , Skorko Maciej , Gola Mateusz , Bielecki Maksymilian
Published: May 2024

Background

Substance Use Disorder (SUD) persists as a significant public health challenge worldwide, with an estimated prevalence of approximately 10-15% across the global populace. This condition is characterized by a notably high risk of lapses and relapses, even subsequent to treatment interventions. Mobile health interventions, owing to their widespread accessibility, emerge as a promising approach to diminish the risk of relapse post-treatment and to broaden the scope of care, especially in regions with a scarcity of trained medical professionals.

Method

This study is designed to assess the effectiveness of mobile interventions in mitigating cravings and preventing lapses among individuals diagnosed with SUD. Employing a two-armed, randomized controlled trial framework, the study will evaluate a self-administered psychological intervention delivered through a mobile application, Nałogometr 2.0. Over a period of three months, participants will engage with intervention modules that primarily incorporate mindfulness techniques and Cognitive Behavioral Therapy (CBT) principles. Ecological Momentary Assessment (EMA) will be utilized to gather longitudinal data on a range of variables that are indicative of craving intensity and the risk of lapse. In addition to this, a monthly-administered battery of questionnaires will be employed to gauge the severity of substance dependence, as well as to measure levels of anxiety, depression, and overall life satisfaction.

Results

Results will be submitted for publication in peer-reviewed journals.

Clinical trial registration

https://clinicaltrials.gov/, identifier [NCT05730504].
Methylphenidate, but not citalopram, decreases impulsive choice in rats performing a temporal discounting task
Authors: Koloski Miranda F. , Terry Alyssa , Lee Noelle , Ramanathan Dhakshin S.
Published: May 2024

Introduction

Drugs targeting monoamine systems remain the most common treatment for disorders with impulse control impairments. There is a body of literature suggesting that drugs affecting serotonin reuptake and dopamine reuptake can modulate distinct aspects of impulsivity – though such tests are often performed using distinct behavioral tasks prohibiting easy comparisons.

Methods

Here, we directly compare pharmacologic agents that affect dopamine (methylphenidate) vs serotonin (citalopram) manipulations on choice impulsivity in a temporal discounting task where rats could choose between a small, immediate reward or a large reward delayed at either 2 or 10s. In control conditions, rats preferred the large reward at a small (2s) delay and discounted the large reward at a long (10s) delay.

Results

Methylphenidate, a dopamine transport inhibitor that blocks reuptake of dopamine, dose-dependently increased large reward preference in the long delay (10s) block. Citalopram, a selective serotonin reuptake inhibitor, had no effect on temporal discounting behavior. Impulsive behavior on the temporal discounting task was at least partially mediated by the nucleus accumbens shell. Bilateral lesions to the nucleus accumbens shell reduced choice impulsivity during the long delay (10s) block. Following lesions, methylphenidate did not impact impulsivity.

Discussion

Our results suggest that striatal dopaminergic systems modulate choice impulsivity via actions within the nucleus accumbens shell, whereas serotonin systems may regulate different aspects of behavioral inhibition/impulsivity.
Broadband Noise Reduction of a Two-Stage Fan with Wavy Trailing-Edge Blades
Authors: Gao Ruibiao , Chen Weijie , Tong Hang , Lian Jianxin , Qiao Weiyang
Published: May 2024

In this paper, a numerical investigation is performed to study the broadband noise of a fan stage with wavy trailing-edge blades. A study of the wavelength and ratio of amplitude to wavelength (H/L) is conducted to better understand the noise reduction effect of wavy trailing-edge blades. A rotor–stator interaction broadband noise prediction method based on the result of a Reynolds-averaged Navier–Stokes equation is used. The results show that all wavy trailing-edge configurations reduce the sound power level of the fan stage. The noise reduction effect of H20L10 is the best among all the wavy trailing-edge configurations, and the sound power level is reduced by 2.4 dB at 1000 Hz. When the H/L remains unchanged, the noise reduction effect of the wavy trailing-edge configuration increases with the increase in wavelength. When the wavelength remains unchanged, the noise reduction effect of the wavy trailing-edge configuration with an H/L of 2 is the best. The use of wavy trailing-edge configurations reduces the turbulent kinetic energy and turbulent integral length scale upstream of the stator by changing the wake of the rotor, thereby reducing the rotor–stator interaction broadband noise of the fan stage.
Study and Experiment on Screen Surface Homogenization Technology of Dislodged Material Based on Longitudinal Flow Threshing
Authors: Ming Jiarui , He Qinghao , Yue Dong , Ma Jie , Wang Yanan , Yin Jianning , Cui Yipeng , Geng Duanyang
Published: May 2024

Aiming at the problems of uneven distribution of dislodged material on the screen surface of longitudinal axial flow grain combine harvester, a large difference in material clearing time, and large clearing loss, a dislodged material homogenizing device that can realize dislodged material return and homogenization at the rear of longitudinal axial flow was developed. (1) The structure and motion parameters of the reflux plate were determined, and simulation tests were carried out to verify them; (2) A test bench was set up, and the Box-Behnken test method was adopted to determine the influence law of each factor on the operating effect and the optimal parameter combination, and the results showed that the tilt angle of the return plate, motor speed, and amplitude had a significant influence on the distribution uniformity of the material on the screen surface; it was determined that the optimal combination of the angle of the return plate configuration was 28.7°, the speed of the motor was 247 r/min, the amplitude of the return plate was 18.3 mm, and the seed contamination rate was 0.48%. The optimum combination was determined to be 28.7°, 247 r/min, 18.3 mm, and 0.48% impurity rate; (3) under the conditions of the field test validation, the validation error is less than 5%, proving that it can effectively improve the performance of the clearing and reduce the rate of impurity content.
Appropriate Application of Organic Fertilizer Can Effectively Improve Soil Environment and Increase Maize Yield in Loess Plateau
Authors: Xing Yingying , Li Yuan , Zhang Fan , Wang Xiukang
Published: May 2024

Fertilization has a significant impact on soil nutrients and microbiological properties, which, in turn, affect crop yield. However, the specific effects of organic and inorganic fertilizers on soil fertility and microbial characteristics in maize fields, as well as the key soil factors influencing changes in crop yield, remain largely unknown. A study was conducted over two years (2021–2022) to investigate the impact of various nitrogen fertilization rates and types on maize yield and soil properties in the Loess Plateau. Field experiments with five nitrogen levels (60, 90, 120, 150, and 180 kg N ha−1) and two fertilizer types (chemical and organic) were conducted. The optimal yield was achieved with 150 kg N ha−1, significantly surpassing that of other treatments by 4.5−45.7%. Compared with the organic fertilizers, the chemical fertilizers increased soil salt and catalase levels, with soil nitrate and ammonium content being higher at the jointing stage but lower at maturity. Organic fertilization improved soil potassium, organic matter, urease and phosphatase activities, and microbial populations. Yield correlated with several soil indicators, including salt content, nitrate, available potassium, and enzyme activities. Notably, soil nitrate also correlated with actinomyces quantity. A principal component analysis showed that the organic fertilizer was more beneficial to soil health than the chemical fertilizer. Consequently, this study recommends 150 kg N ha−1 of organic fertilizer for sustainable maize farming and soil health in China’s northwest arid region, providing a theoretical framework for agricultural practices.
Robotics Perception: Intention Recognition to Determine the Handball Occurrence during a Football or Soccer Match
Authors: Hassan Mohammad Mehedi , Karungaru Stephen , Terada Kenji
Published: May 2024

In football or soccer, a referee controls the game based on the set rules. The decisions made by the referee are final and can’t be appealed. Some of the decisions, especially after a handball event, whether to award a penalty kick or a yellow/red card can greatly affect the final results of a game. It is therefore necessary that the referee does not make an error. The objective is therefore to create a system that can accurately recognize such events and make the correct decision. This study chose handball, an event that occurs in a football game (Not to be confused with the game of Handball). We define a handball event using object detection and robotic perception and decide whether it is intentional or not. Intention recognition is a robotic perception of emotion recognition. To define handball, we trained a model to detect the hand and ball which are primary objects. We then determined the intention using gaze recognition and finally combined the results to recognize a handball event. On our dataset, the results of the hand and the ball object detection were 96% and 100% respectively. With the gaze recognition at 100%, if all objects were recognized, then the intention and handball event recognition were at 100%.
Quantification of Recycled PET in Commercial Bottles by IR Spectroscopy and Chemometrics
Authors: Zappi Alessandro , Biancolillo Alessandra , Kassouf Nicholas , Marassi Valentina , Morozzi Pietro , Tositti Laura , Melucci Dora
Published: May 2024

A novel approach for the quantification of recycled polyethylene terephthalate (r-PET) in commercial bottles is presented. Fifty-eight bottle samples from several brands and producers containing different percentages of r-PET were purchased from the market. Samples were analyzed by two spectroscopic methods: near-infrared (NIR) spectroscopy and attenuated total reflection (ATR) spectroscopy in the mid-infrared (MIR) region. No chemical pre-treatment was applied before analyses. The spectra were analyzed by partial-least squares (PLS) regression, and two models for NIR and MIR data were computed. Then, a multi-block regression was applied to join the two datasets. All models were validated by cross-validation and by excluding and projecting onto the model the replicated spectra of one sample at a time. Results demonstrated the potential of this approach, especially considering the variability of commercial samples in terms of additives, shape, or thickness of the bottles: for samples close to the centroids of the models (i.e., from 10 to 50% r-PET), the predictions of multi-block method seldom departed from the expected values of ±10%. Only for samples with 0% declared r-PET, the models showed poor prediction abilities.
Reproductive Activity of Socorro Island Merino Ewes and Their Crosses with Pelibuey under Heat Stress Conditions
Authors: García-Casillas Arturo César , Prado-Rebolledo Omar Francisco , Carrillo-Díaz María Isabel , Zepeda-Batista José Luis , Barajas-Saucedo Carlos Eduardo , Hernández-Rivera Juan Augusto
Published: May 2024

An experiment was carried out to evaluate the effect of spring and autumn seasons on the reproductive activity of Merino Socorro Island ewes and their crosses with Pelibuey under heat stress (HS) conditions in the tropics. All ewes (n = 80) were randomly assigned to one of two breeds during the first and second periods, respectively: (1) Twenty Socorro Island Merino ewes (SIM) and (2) 20 Pelibuey Crossbred ewes (PBC). Animals were fed the same diet and given water ad libitum. All statistical analyses were performed using SAS statistical software 9.12 procedures. In both seasons, a mean of more than 80 U of maximum THI was obtained, while in spring and autumn, the minimum THI exceeded 30 and 40 U, respectively. All animals were in oestrus and ovulated in both seasons. The frequency of animals in spring during the first 48 h of oestrus expression was greater (p < 0.05) than 48–55 h but similar (p > 0.05) than 55–65 h; in autumn during the first 48 h and 48–55 h were similar (p > 0.05), but different (p < 0.05) than 55–65 h. The duration of oestrus expression was longer in the spring than in the autumn (p < 0.05). The frequency of animals was higher (p < 0.05) in SIM than in PBC ewes during the first oestrus cycle (1–17 d) and was also higher (p < 0.05) in PBC than in SIM ewes during the second oestrus cycle (18–35 d). The SIM ewes produced more (p < 0.05) progesterone (P4) than the PBC ewes. During the sampling days of the oestrus cycle, more P4 was created in autumn than in spring (p < 0.05). Both breeds showed severe HS. In the future, ewes treated under assisted reproductive programs in the tropics may improve reproductive efficiency.
The Expression and Epigenetic Characteristics of the HSF2 Gene in Cattle-Yak and the Correlation with Its Male Sterility
Authors: Yang Qinhui , Xie Yumian , Pan Bangting , Cheng Yuying , Zhu Yanjin , Fei Xixi , Li Xupeng , Yu Jun , Chen Zhuo , Li Jian , Xiong Xianrong
Published: May 2024

Aberrant expression of the heat shock proteins and factors was revealed to be closely associated with male reproduction. Heat shock factor 2 (HSF2) is a transcription factor that is involved in the regulation of diverse developmental pathways. However, the role and the corresponding molecular mechanism of HSF2 in male cattle-yak sterility are still poorly understood. Therefore, the aim of this study was to obtain the sequence and the biological information of the cattle-yak HSF2 gene and to investigate the spatiotemporal expression profiles of the locus during the development of cattle-yak testes. Additionally, the differential expression was analyzed between the cattle-yak and the yak, and the methylation of corresponding promoter regions was compared. Our results showed an additional 54 bp fragment and a missense mutation (lysine to glutamic acid) were presented in the cattle-yak HSF2 gene, which correlated with enriched expression in testicular tissue. In addition, the expression of the HSF2 gene showed dynamic changes during the growth of the testes, reaching a peak in adulthood. The IHC indicated that HSF2 protein was primarily located in spermatocytes (PS), spermatogonia (SP), and Sertoli cells (SC) in cattle-yak testes, compared with the corresponding cells of cattle and the yak. Furthermore, bisulfite-sequencing PCR (BSP) revealed that the methylated CpG sites in the promoter region of the cattle-yak HSF2 were more numerous than in the yak counterpart, which suggests hypermethylation of this region in the cattle-yak. Taken together, the low expression abundance and hypermethylation of HSF2 may underpin the obstruction of spermatogenesis, which leads to male cattle-yak infertility. Our study provided a basic guideline for the HSF2 gene in male reproduction and a new insight into the mechanisms of male cattle-yak sterility.
Seasonal and Spatial Distribution Characteristics of Sepia esculenta in the East China Sea Region: Transfer of the Central Distribution from 29° N to 28° N
Authors: Xu Min , Yang Linlin , Liu Zunlei , Zhang Yi , Zhang Hui
Published: May 2024

The golden cuttlefish (Sepia esculenta) is an important cephalopod species with a lifespan of approximately one year. The species plays a crucial role in marine ecological support services and is commercially valuable in fisheries. In the seas around China, this species has emerged as the main target for cuttlefish fisheries, replacing Sepiella maindroni since the 1990s. Variations in oceanographic conditions associated with global warming could significantly impact the temporal-spatial distribution of the species. In this study, we performed bottom trawling surveys with four cruises during 2018–2019 in the East China Sea region to determine the current resource status and seasonal-spatial variations in S. esculenta. We found that the average individual weight (AIW) values were 4.87 and 519.00 g/ind at stations located at 30.50° N, 124.00° E and 30.50° N, 124.50° E, respectively, with the aggregation of larvae and parent groups in spring. The species was not distributed north of 32.00° N in summer. The catch per unit effort by weight (CPUEw) value decreased in the order of 2772.50→2575.20→503.29→124.36 g/h, corresponding to latitudes of 34.50° N→34.00° N→33.50° N→32.50° N 121.50° E in autumn. The most suitable fishing areas were the south of the East China Sea region in spring; the south of the East China Sea region extending to the center and outer parts of the East China Sea region in summer; the south of the Yellow Sea close to the Haizhou Bay fishing ground and the forbidden fishing line region of the Lusi and Dasha fishing grounds in autumn; and the south and center of the East China Sea region in winter. The most suitable sea bottom temperature (SBT) values from spring to winter were 14.76–20.53 °C, 19.54–22.98 °C, 11.79–17.64 °C, and 16.94–20.36 °C, respectively. The most suitable sea bottom salinity (SBS) values were 31.53–34.80‰ in spring, 32.95–34.68‰ in summer, 31.51–34.77‰ in autumn, and 33.82–34.51‰ in winter. We concluded the following: (1) the southern and northern areas of the East China Sea region are spawning and nursery grounds, respectively, in spring; (2) the central distribution is located at a latitude of 28.00° N in autumn and winter; and (3) the southern area of the Yangtze River to the north is a spawning ground in spring, and the areas located at 29.00–34.50° N, 124.00–124.50° E, and 28.00–30.50° N, 125.50–126.50° E are nursery grounds. The results of this study provide useful guidance for appropriate fisheries management, thereby avoiding a collapse in the S. esculenta population, which has been experienced in other species in this area.
Alcohol Triggers the Accumulation of Oxidatively Damaged Proteins in Neuronal Cells and Tissues
Authors: Mudyanselage Anusha W. , Wijamunige Buddhika C. , Kocoń Artur , Turner Ricky , McLean Denise , Morentin Benito , Callado Luis F. , Carter Wayne G.
Published: May 2024

Alcohol is toxic to neurons and can trigger alcohol-related brain damage, neuronal loss, and cognitive decline. Neuronal cells may be vulnerable to alcohol toxicity and damage from oxidative stress after differentiation. To consider this further, the toxicity of alcohol to undifferentiated SH-SY5Y cells was compared with that of cells that had been acutely differentiated. Cells were exposed to alcohol over a concentration range of 0–200 mM for up to 24 h and alcohol effects on cell viability were evaluated via MTT and LDH assays. Effects on mitochondrial morphology were examined via transmission electron microscopy, and mitochondrial functionality was examined using measurements of ATP and the production of reactive oxygen species (ROS). Alcohol reduced cell viability and depleted ATP levels in a concentration- and exposure duration-dependent manner, with undifferentiated cells more vulnerable to toxicity. Alcohol exposure resulted in neurite retraction, altered mitochondrial morphology, and increased the levels of ROS in proportion to alcohol concentration; these peaked after 3 and 6 h exposures and were significantly higher in differentiated cells. Protein carbonyl content (PCC) lagged behind ROS production and peaked after 12 and 24 h, increasing in proportion to alcohol concentration, with higher levels in differentiated cells. Carbonylated proteins were characterised by their denatured molecular weights and overlapped with those from adult post-mortem brain tissue, with levels of PCC higher in alcoholic subjects than matched controls. Hence, alcohol can potentially trigger cell and tissue damage from oxidative stress and the accumulation of oxidatively damaged proteins.
Study on Non-Stationary Wind Speed Models and Wind Load Design Parameters Based on Data from the Beijing 325 m Meteorological Tower, 1991–2020
Authors: Yang Shihan , Ji Xiaodong , Tao Yifan , Jia Jingjing , Zhang Zeyu , Jiang Li
Published: May 2024

As the capital of China and a densely populated major city, the characteristics of Beijing’s near-surface wind field change significantly with the increase in the density of underlying urban structures. The high randomness of natural wind makes it extremely difficult to develop a universally applicable wind-resistant load design code based on topographic factors and architectural features. This article takes the wind speeds recorded at 15 different height levels within the urban area by the 325 m meteorological tower in Beijing from 1991 to 2020 as the research subject. It quantifies the wind speed trends at different heights and introduces time-varying functions to establish a non-stationary wind speed model based on the optimal model. Additionally, it compares the basic wind speeds and wind pressure height variation coefficients obtained from measurements with the standards. The results show that, during the past 30 years of urbanization, the near-surface wind speed in the Beijing area has shown a decreasing trend. The model incorporating time-varying functions exhibited the best fit and demonstrated good predictive capabilities, with its calculated basic wind speeds being relatively high. The wind pressure height variation coefficient values in Beijing are between Class C and Class D terrains, being closer to Class C at lower altitudes. The conclusion reveals that urbanization has a significant impact on wind speeds, primarily concentrated at lower height levels, and that the basic wind speeds calculated based on standards underestimate the actual conditions when this impact is not considered. Although the average wind speed’s wind profile index across the entire time series is mostly greater than the fixed value of 0.3 given by Class D, this represents an overestimated wind profile index for maximum wind speeds.
Recommendations for the Model-Based Systems Engineering Modeling Process Based on the SysML Model and Domain Knowledge
Authors: Zhang Jia , Yang Shuqun
Published: May 2024

Model-based systems engineering (MBSE) is a modeling approach used in industry to support the formalization, analysis, design, checking and verification of systems. In MBSE modeling, domain knowledge is the basis of the modeling. However, modeling does not happen overnight; it requires systematic training and a significant investment of resources. Unfortunately, many domain experts lack the expertise required for modeling, even though they know the domain well. The question arises about how to provide system modelers with domain knowledge at the right time to support the efficient completion of modeling. Since MBSE research that integrates AI is just beginning to take off, no public dataset is available. In this paper, aerospace SysML models are constructed based on spacecraft-related domain knowledge to form SysML model data. The validation rules are studied to validate the SysML model data, and combined with the concept of the recommended system, a recommendation method for the MBSE modeling process based on the knowledge and SysML model is proposed. A GLOVE language model is pre-trained by using domain knowledge and general knowledge; the model data are also used to fine-tune the GLOVE language model combined with the pre-training to recommend some domain development processes. The recommendation list is manually quality-verified and fed into the pre-training phase, while new requirement texts are continuously added in the fine-tuning phase, resulting in a more relevant and accurate recommendation list. Experiments show that the incremental recommender system can not only effectively recommend SysML models, but also improve the quality and efficiency of MBSE development.
Architects’ Perception of Quality of Life—Impact, Practice, and Barriers
Authors: Jacobi Stine Lea , Bjørner Thomas
Published: May 2024

This study intended to identify the perception of quality of life (QoL) among architects, how architecture can impact QoL, and which barriers architects perceive to impact QoL. Numerous studies have emphasized the significance of the built environment in determining QoL, especially in institutional buildings. However, there has been less focus on how architects perceive QoL and how the concept is applied in their planning and design for residential buildings. The contribution of this study is to provide an increasingly important awareness of how to improve the architects’ considerations to build for QoL. The study is based on qualitative data from in-depth interviews with ten architects and one workshop with seven architects. The participants were selected by quota sampling and were all partners or owners of Danish architectural firms that provide housing services and are representative of the Danish architectural industry. The results reveal that the perceptions of QoL among architects are linked to three primary dimensions: health, a sense of harmony, and the experience of enchantment. The participants perceived that architectural design could impact QoL in three primary dimensions: the environment, the experience of enchantment, and health. The most frequent perceived barriers are linked to the economy and resources, building codes and regulations, and knowledge and communication.
The Royal Chapel of Pedro I of Castile in the Christianised Mosque of Seville
Authors: Gumiel-Campos Pablo
Published: May 2024

Pedro I of Castile (1350–1369) founded a royal chapel in the Christianised Mosque of Seville. He intended to house there his body, that of Queen María de Padilla, and their son the Infant Alfonso (1359–1362). This mausoleum is well documented both in the king’s will and in the chronicles of López de Ayala; however, there are no material remains as it was demolished with the construction of the new cathedral in the 15th century. In this article, we seek to produce a state of the art history of the building, a compilation of all the documentary sources that exist for its analysis, and an approach to the problems that hinder its study. We have also tried, unsuccessfully, to put forward a hypothesis about its original location, but we have come up against a dead end. Despite this, we consider it essential to lay all the cards on the table and prevent the mausoleum from falling into oblivion.
Morphology and Composition of Lumbar Intervertebral Discs: Comparative Analyses of Manual Measurement and Computer-Assisted Algorithms
Authors: Cheng Yiting , Ma Yuyan , Li Kang , Gungor Celal , Sesek Richard , Tang Ruoliang
Published: May 2024

Background: The morphology and internal composition, particularly the nucleus-to-cross sectional area (NP-to-CSA) ratio of the lumbar intervertebral discs (IVDs), is important information for finite element models (FEMs) of spinal loadings and biomechanical behaviors, and, yet, this has not been well investigated and reported. Methods: Anonymized MRI scans were retrieved from a previously established database, including a total of 400 lumbar IVDs from 123 subjects (58 F and 65 M). Measurements were conducted manually by a spine surgeon and using two computer-assisted segmentation algorithms, i.e., fuzzy C-means (FCM) and region growing (RG). The respective results were compared. The influence of gender and spinal level was also investigated. Results: Ratios derived from manual measurements and the two computer-assisted algorithms (FCM and RG) were 46%, 39%, and 38%, respectively. Ratios derived manually were significantly larger. Conclusions: Computer-assisted methods provide reliable outcomes that are traditionally difficult for the manual measurement of internal composition. FEMs should consider the variability of NP-to-CSA ratios when studying the biomechanical behavior of the spine.
Are Young People with Turner Syndrome Who Have Undergone Treatment with Growth and Sex Hormones at Higher Risk of Metabolic Syndrome and Its Complications?
Authors: Krzyścin Mariola , Sowińska-Przepiera Elżbieta , Gruca-Stryjak Karolina , Soszka-Przepiera Ewelina , Syrenicz Igor , Przepiera Adam , Bumbulienė Žana , Syrenicz Anhelli
Published: May 2024

Introduction: Metabolic syndrome (MetS), characterized by visceral obesity, glucose abnormalities, hypertension and dyslipidemia, poses a significant risk of diabetes and cardiovascular disease. Turner syndrome (TS), resulting from X chromosome abnormalities, carries health complications. Despite growing evidence of an increased risk of MetS in women with TS, its prevalence and risk factors remain under investigation. These considerations are further complicated by the varying timing and dosages of treatment with growth hormone and sex hormones. Methods: We conducted a cross-sectional study comparing 44 individuals with TS with 52 age-matched control subjects. Growth hormone treatment in the study group was administered for varying lengths of time, depending on clinical response. We collected anthropometric, metabolic, endocrine and body composition data. Statistical analyses included logistic regression. Results: Baseline characteristics, including age, BMI and height, were comparable between the TS and control groups. Hormonally, individuals with TS showed lower levels of testosterone, DHEA-S, and cortisol, as well as elevated FSH. Lipid profiles indicated an atherogenic profile, and the body composition analysis showed increased visceral adipose tissue in those with TS. Other metabolic abnormalities were common in individuals with TS too, including hypertension and impaired fasting glucose levels. The risk of MetS components was assessed in subgroups according to karyotypes: monosomy 45X0 vs. other mosaic karyotypes. Logistic regression analysis showed a significant association between increased visceral adipose tissue in subjects with TS. Those with metabolic complications tended to have less muscle strength compared to those without these complications in both the study and control groups. Conclusions: This study highlights the unique metabolic and cardiovascular risk profile of individuals with TS, characterized by atherogenic lipids, higher levels of visceral adipose tissue and increased metabolic abnormalities. These findings underscore the importance of monitoring metabolic health in individuals with TS, regardless of age, BMI or karyotype, and suggest the potential benefits of lifestyle modification, building more muscle strength, and weight control strategies. Further research is needed to better understand and address the metabolic challenges faced by women with TS.
Dysbiosis Signature of Fecal Microbiota in Patients with Pancreatic Adenocarcinoma and Pancreatic Intraductal Papillary Mucinous Neoplasms
Authors: Sidiropoulos Theodoros , Dovrolis Nikolas , Katifelis Hector , Michalopoulos Nikolaos V. , Kokoropoulos Panagiotis , Arkadopoulos Nikolaos , Gazouli Maria
Published: May 2024

Pancreatic cancer (PC) ranks as the seventh leading cause of cancer-related deaths, with approximately 500,000 new cases reported in 2020. Existing strategies for early PC detection primarily target individuals at high risk of developing the disease. Nevertheless, there is a pressing need to identify innovative clinical approaches and personalized treatments for effective PC management. This study aimed to explore the dysbiosis signature of the fecal microbiota in PC and potential distinctions between its Intraductal papillary mucinous neoplasm (IPMN) and pancreatic ductal adenocarcinoma (PDAC) phenotypes, which could carry diagnostic significance. The study enrolled 33 participants, including 22 diagnosed with PDAC, 11 with IPMN, and 24 healthy controls. Fecal samples were collected and subjected to microbial diversity analysis across various taxonomic levels. The findings revealed elevated abundances of Firmicutes and Proteobacteria in PC patients, whereas healthy controls exhibited higher proportions of Bacteroidota. Both LEfSe and Random Forest analyses indicated the microbiome’s potential to effectively distinguish between PC and healthy control samples but fell short of differentiating between IPMN and PDAC samples. These results contribute to the current understanding of this challenging cancer type and highlight the applications of microbiome research. In essence, the study provides clear evidence of the gut microbiome’s capability to serve as a biomarker for PC detection, emphasizing the steps required for further differentiation among its diverse phenotypes.
APPROACH: Sensitive Detection of Exosomal Biomarkers by Aptamer-Mediated Proximity Ligation Assay and Time-Resolved Förster Resonance Energy Transfer
Authors: Li Ying , Qian Meiqi , Liu Yongpeng , Qiu Xue
Published: May 2024

Exosomal biomarker detection holds great importance in the field of in vitro diagnostics, offering a non-invasive and highly sensitive approach for early disease detection and personalized treatment. Here, we proposed an “APPROACH” strategy, combining aptamer-mediated proximity ligation assay (PLA) with rolling circle amplification (RCA) and time-resolved Förster resonance energy transfer (TR-FRET) for the sensitive and semi-homogenous detection of exosomal biomarkers. PLA probes consisted of a cholesterol-conjugated oligonucleotide, which anchored to the membrane of an exosome, and a specific aptamer oligonucleotide that recognized a target protein of the exosome; the proximal binding of pairs of PLA probes to the same exosome positioned the oligonucleotides in the vicinity of each other, guiding the hybridization and ligation of two subsequently added backbone and connector oligonucleotides to form a circular DNA molecule. Circular DNA formed from PLA underwent rolling circle amplification (RCA) for signal amplification, and the resulting RCA products were subsequently quantified by TR-FRET. The limits of detection provided by APPROACH for the exosomal biomarkers CD63, PD-L1, and HER2 were 0.46 ng∙μL−1, 0.77 ng∙μL−1, and 1.1 ng∙μL−1, respectively, demonstrating excellent analytical performance with high sensitivity and quantification accuracy. Furthermore, the strategy afforded sensitive detection of exosomal CD63 with a LOD of 1.56 ng∙μL−1 in complex biological matrices, which underscored its anti-interference capability and potential for in vitro detection. The proposed strategy demonstrates wide-ranging applicability in quantifying diverse exosomal biomarkers while exhibiting robust analytical characteristics, including high sensitivity and accuracy.