bluuebunny/crossref_metadata_2025 huggingface dataset:
Growth and bile tolerance ofLactobacillus brevisstrains isolated from Japanese pickles in artificial digestive juices and contribution of cell-bound exopolysaccharide to cell aggregation
Authors: Suzuki Shigenori , Honda Hiroyuki , Suganuma Hiroyuki , Saito Tadao , Yajima Nobuhiro
Published: January 2014

Cell-bound exopolysaccharide (EPS) of the aggregable strain Lactobacillus brevis KB290 isolated from traditional Japanese pickles has been reported to protect against the effects of bile. However, there are no reports of bile tolerance mechanisms for other L. brevis strains that have aggregability. To elucidate the mechanism of bile tolerance of L. brevis KB290, we found 8 aggregable L. brevis strains out of 121 L. brevis strains isolated from traditional Japanese fermented pickles. We estimated their growth in artificial digestive juice and the amount of cell-bound EPS. We found 3 types of aggregation for these strains: filiform (<1 mm), medium floc (1–5 mm), or large floc (>5 mm). There was no significant difference in growth between nonaggregable and aggregable strains in the artificial digestive juice. The large floc strains selected from the aggregation strains showed significantly higher growth in the artificial digestive juice than nonaggregable strains. In medium and large floc strains, cell-bound EPS, mainly consisting of glucose, N-acetylglucosamine, and N-acetylmannosamine, were observed. The amount of EPS and each strain’s growth index showed a positive correlation. We conclude that aggregable L. brevis strains were also protected by cell-bound EPS.
Probing periodic oscillations in a silane dusty plasma in a very high-frequency plasma enhanced chemical vapor deposition process
Authors: Mohan A. , van der Wel C.M. , Schropp R.E.I. , Rath J.K.
Published: January 2014

To estimate the dust formation time scale in a silane–hydrogen plasma, optical and electrical plasma diagnostics are performed. We report a periodic fluctuation in emission intensity and electric current in a dusty plasma. The trends of the frequency of fluctuations with varying substrate temperatures and gas flows are studied. However, no such fluctuation is observed in the nondusty plasma. It is hypothesized that this fluctuation arises from the periodic formation and ejection of a dust cloud via the void formation when a critical dust size is reached.
A NOVEL CRM PFC CONTROL METHOD FOR REDUCING INDUCTOR
Authors: SHI LING-FENG , GUAN HUI-LI , LI QIN-QIN , LAI XIN-QUAN
Published: January 2014

A novel control method for the critical conduction mode (CRM) power factor correction (PFC) converter is presented, which reduces the size of the boost inductor in the system with wide input-voltage range and improves the efficiency of the system with low input voltage. By introducing the following boost circuit, the output voltage in the application circuit varies with the input root mean square (RMS) voltage to reduce the demand for the large size of the inductor and the efficiency of the system keeps high under the low input voltage. A novel CRM PFC control system with smaller size inductor and higher efficiency is achieved by applied the following boost method to the core control circuits. Experiment results show that the inductance value of the boost inductor is 430 μH using the presented PFC control system and 700 μH using the traditional PFC control system when the input voltage varies from 85 V to 265 V. The novel control method decreases the inductor's value at 38.2%, and the efficiency of the system improves at 1.62% under the input voltage of 85 V.
USING AN IMPROVED BEE MEMORY DIFFERENTIAL EVOLUTION ALGORITHM FOR PARAMETER ESTIMATION TO SIMULATE BIOCHEMICAL PATHWAYS
Authors: CHONG CHUII KHIM , MOHAMAD MOHD SABERI , DERIS SAFAAI , SHAMSIR MOHD SHAHIR , CHAI LIAN EN , CHOON YEE WEN
Published: January 2014

When analyzing a metabolic pathway in a mathematical model, it is important that the essential parameters are estimated correctly. However, this process often faces few problems like when the number of unknown parameters increase, trapping of data in the local minima, repeated exposure to bad results during the search process and occurrence of noisy data. Thus, this paper intends to present an improved bee memory differential evolution (IBMDE) algorithm to solve the mentioned problems. This is a hybrid algorithm that combines the differential evolution (DE) algorithm, the Kalman filter, artificial bee colony (ABC) algorithm, and a memory feature. The aspartate and threonine biosynthesis pathway, and cell cycle pathway are the metabolic pathways used in this paper. For three production simulation pathways, the IBMDE managed to robustly produce the estimated optimal kinetic parameter values with significantly reduced errors. Besides, it also demonstrated faster convergence time compared to the Nelder–Mead (NM), simulated annealing (SA), the genetic algorithm (GA) and DE, respectively. Most importantly, the kinetic parameters that were generated by the IBMDE have improved the production rates of desired metabolites better than other estimation algorithms. Meanwhile, the results proved that the IBMDE is a reliable estimation algorithm.
IMPLEMENTATION OF THE LORENTZ–DRUDE MODEL INCORPORATED FDTD METHOD ON MULTIPLE GPUs FOR PLASMONICS APPLICATIONS
Authors: AHMED IFTIKHAR , GOH RICK SIOW MONG , KHOO ENG HUAT , LEE KIM HUAT , ZHONG SIAW KIAN , LI ERPING , HUNG TERENCE
Published: January 2014

The Lorentz–Drude model incorporated Maxwell equations are simulated by using the three-dimensional finite difference time domain (FDTD) method and the method is parallelized on multiple graphics processing units (GPUs) for plasmonics applications. The compute unified device architecture (CUDA) is used for GPU parallelization. The Lorentz–Drude (LD) model is used to simulate the dispersive nature of materials in plasmonics domain and the auxiliary differential equation (ADE) approach is used to make it consistent with time domain Maxwell equations. Different aspects of multiple GPUs for the FDTD method are presented such as comparison of different numbers of GPUs, transfer time in between them, synchronous, and asynchronous passing. It is shown that by using multiple GPUs in parallel fashion, significant reduction in the simulation time can be achieved as compared to the single GPU.
Porphyrins as substrates in CuAAC — exclusion of unwanted copper insertion into the macrocyclic core
Authors: Chromiński Mikołaj , Zieleniewska Anna , Karczewski Maksymilian , Gryko Dorota
Published: January 2014

Unwanted copper insertion into a free base porphyrin starting material during Cu -catalyzed [1,3] azide-alkyne dipolar cycloaddition ( CuAAC ) is not observed when using (1,10-phenanthroline)-bis(triphenylphosphine)copper(I) nitrate as a catalyst. Reactions give 1,4-disubstituted 1,2,3-triazoles in good yields for a broad range of porphyrins. 1,5-Disubstituted 1,2,3-triazole cycloadducts can be generated in the presence of Cp*RuCl ( COD ).
Prevalence and Predictors of Maternal Anemia during Pregnancy in Gondar, Northwest Ethiopia: An Institutional Based Cross-Sectional Study
Authors: Melku Mulugeta , Addis Zelalem , Alem Meseret , Enawgaw Bamlaku
Published: January 2014

Background. Anaemia is a global public health problem which has an eminence impact on pregnant mother. The aim of this study was to assess the prevalence and predictors of maternal anemia.Method. A cross-sectional study was conducted from March 1 to April 30, 2012, on 302 pregnant women who attended antenatal care at Gondar University Hospital. Interview-based questionnaire, clinical history, and laboratory tests were used to obtain data. Bivariate and multivariate logistic regression was used to identify predictors.Result. The prevalence of anemia was 16.6%. Majority were mild type (64%) and morphologically normocytic normochromic (76%) anemia. Anemia was high at third trimester (18.9%). Low family income (AOR [95% CI] = 3.1 [1.19, 8.33]), large family size (AOR [95% CI] = 4.14 [4.13, 10.52]),hookworminfection (AOR [95% CI] = 2.72 [1.04, 7.25]), andHIVinfection (AOR [95% CI] = 5.75 [2.40, 13.69]) were independent predictors of anemia.Conclusion. The prevalence of anemia was high; mild type and normocytic normochromic anemia was dominant. Low income, large family size,hookworminfection, and HIV infection were associated with anemia. Hence, efforts should be made for early diagnosis and management ofHIVandhookworminfection with special emphasis on those having low income and large family size.
The Incidence and Predictors of Thromboembolic Events in Patients with Lung Cancer
Authors: Kadlec Bohdan , Skrickova Jana , Merta Zdenek , Dusek Ladislav , Jarkovsky Jiri
Published: January 2014

Patients with lung cancer experience elevated risk of venous thromboembolism. Cancer patients with thrombosis have a shorter life expectancy and the occurrence of VTE worsens the quality of life and may delay, interrupt, or completely halt the cancer therapy. In a large cohort of lung cancer patients we monitored the incidence of venous thromboembolism and we identified groups of patients with the highest risk of venous thromboembolism suitable for antithrombotic prophylaxis, which could favourably affect their morbidity and mortality.
CC Chemokine Receptor 5: The Interface of Host Immunity and Cancer
Authors: Oliveira Carlos Eduardo Coral de , Oda Julie Massayo Maeda , Losi Guembarovski Roberta , Oliveira Karen Brajão de , Ariza Carolina Batista , Neto Jamil Soni , Banin Hirata Bruna Karina , Watanabe Maria Angelica Ehara
Published: January 2014

Solid tumors are embedded in a stromal microenvironment consisting of immune cells, such as macrophages and lymphocytes, as well as nonimmune cells, such as endothelial cells and fibroblasts. Chemokines are a type of small secreted chemotactic cytokine and together with their receptors play key roles in the immune defense. Critically, they regulate cancer cellular migration and also contribute to their proliferation and survival. The CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. Additionally, CCR5 may have an indirect effect on cancer progression by controlling the antitumor immune response, since it has been demonstrated that its expression could promote tumor growth and contribute to tumor metastasis, in different types of malignant tumors. Furthermore, it was demonstrated that a CCR5 antagonist may inhibit tumor growth, consisting of a possible therapeutic target. In this context, the present review focuses on the establishment of CCR5 within the interface of host immunity, tumor microenvironment, and its potential as a targeting to immunotherapy.
The NOMAD Experiment at CERN
Authors: Vannucci F.
Published: January 2014

The purpose of this paper is to review the experimental apparatus and some physics results from the NOMAD (neutrino oscillation magnetic detector) experiment which took data in the CERN wide-band neutrino beam from 1995 to 1998. It collected and reconstructed more than one million charged current (CC)νμevents with an accuracy which was previously obtained only with bubble chambers. The main aim of the experiment was to search for the oscillationνμintoντ, in a region of mass compatible with the prescriptions of the hot dark matter hypothesis, which predicted aντmass in the range of 1–10 eV/c2. This was done by searching forντCC interactions, observing the production of theτlepton through its various decay modes by using kinematical criteria. In parallel, NOMAD also strongly contributed to the study of more conventional processes: quasielastic events, strangeness production and charm dimuon production, single photon production, and coherent neutral pion production. Exotic searches were also investigated. The paper reviews the neutrino beam, the detector setup, the detector performances, the neutrino oscillation results, the strangeness production, the dimuon charm production, and summarizes other pieces of research.
Lithium Improves Survival of PC12 Pheochromocytoma Cells in High-Density Cultures and after Exposure to Toxic Compounds
Authors: Fabrizi Cinzia , De Vito Stefania , Somma Francesca , Pompili Elena , Catizone Angela , Leone Stefano , Lenzi Paola , Fornai Francesco , Fumagalli Lorenzo
Published: January 2014

Autophagy is an evolutionary conserved mechanism that allows for the degradation of long-lived proteins and entire organelles which are driven to lysosomes for digestion. Different kinds of stressful conditions such as starvation are able to induce autophagy. Lithium and rapamycin are potent autophagy inducers with different molecular targets. Lithium stimulates autophagy by decreasing the intracellular myo-inositol-1,4,5-triphosphate levels, while rapamycin acts through the inhibition of the mammalian target of rapamycin (mTOR). The correlation between autophagy and cell death is still a matter of debate especially in transformed cells. In fact, the execution of autophagy can protect cells from death by promptly removing damaged organelles such as mitochondria. Nevertheless, an excessive use of the autophagic machinery can drive cells to death via a sort of self-cannibalism. Our data show that lithium (used within its therapeutic window) stimulates the overgrowth of the rat Pheochromocytoma cell line PC12. Besides, lithium and rapamycin protect PC12 cells from toxic compounds such as thapsigargin and trimethyltin. Taken together these data indicate that pharmacological activation of autophagy allows for the survival of Pheochromocytoma cells in stressful conditions such as high-density cultures and exposure to toxins.
Successful Gastric Volvulus Reduction and Gastropexy Using a Dual Endoscope Technique
Authors: Jamil Laith H. , Huang Brian L. , Kunkel David C. , Jayaraman Vijay , Soffer Edy E.
Published: January 2014

Gastric volvulus is a life threatening condition characterized by an abnormal rotation of the stomach around an axis. Although the first line treatment of this disorder is surgical, we report here a case of gastric volvulus that was endoscopically managed using a novel strategy. An 83-year-old female with a history of pancreatic cancer status postpylorus-preserving Whipple procedure presented with a cecal volvulus requiring right hemicolectomy. Postoperative imaging included a CT scan and upper GI series that showed a gastric volvulus with the antrum located above the diaphragm. An upper endoscopy was advanced through the pylorus into the duodenum and left in this position to keep the stomach under the diaphragm. A second pediatric endoscope was advanced alongside and used to complete percutaneous endoscopic gastrostomy (PEG) placement for anterior gastropexy. The patient’s volvulus resolved and there were no complications. From our review of the literature, the dual endoscopic technique employed here has not been previously described. Patients who are poor surgical candidates or those who do not require emergent surgery can possibly benefit the most from similar minimally invasive endoscopic procedures as described here.
Approximate Solution of Urysohn Integral Equations Using the Adomian Decomposition Method
Authors: Singh Randhir , Nelakanti Gnaneshwar , Kumar Jitendra
Published: January 2014

We apply Adomian decomposition method (ADM) for obtaining approximate series solution of Urysohn integral equations. The ADM provides a direct recursive scheme for solving such problems approximately. The approximations of the solution are obtained in the form of series with easily calculable components. Furthermore, we also discuss the convergence and error analysis of the ADM. Moreover, three numerical examples are included to demonstrate the accuracy and applicability of the method.
Global Stability of Multigroup SIRS Epidemic Model with Varying Population Sizes and Stochastic Perturbation around Equilibrium
Authors: Fan Xiaoming
Published: January 2014

We discuss multigroup SIRS (susceptible, infectious, and recovered) epidemic models with random perturbations. We carry out a detailed analysis on the asymptotic behavior of the stochastic model; when reproduction numberℛ0>1, we deduce the globally asymptotic stability of the endemic equilibrium by measuring the difference between the solution and the endemic equilibrium of the deterministic model in time average. Numerical methods are employed to illustrate the dynamic behavior of the model and simulate the system of equations developed. The effect of the rate of immunity loss on susceptible and recovered individuals is also analyzed in the deterministic model.
Comparison of Two Powder Processing Techniques on the Properties of Cu-NbC Composites
Authors: Long B. D. , Othman R. , Zuhailawati Hussain , Umemoto M.
Published: January 2014

Anin situCu-NbC composite was successfully synthesized from Cu, Nb, and C powders using ball milling and high pressure torsion (HPT) techniques. The novelty of the new approach, HPT, is the combination of high compaction pressure and large shear strain to simultaneously refine, synthesize, and consolidate composite powders at room temperature. The HPTed Cu-NbC composite was formed within a short duration of 20 min without Fe contamination from the HPT’s die. High porosity of 3–9%, Fe and niobium oxidations, from grinding media and ethanol during ball milling led to low electrical conductivity of the milled Cu-NbC composite. The electrical conductivity of the HPTed Cu-NbC composite showed a value 50% higher than that of milled Cu-NbC composite of the same composition.
Calcium Hydroxide Nanoparticles and Hypogeum Environment: Test to Understand the Best Way of Application
Authors: Macchia Andrea , Bettucci Ottavia , Gravagna Elisa , Ferro Daniela , Albini Romana , Mazzei Barbara , Campanella Luigi
Published: January 2014

For a long time the conservation of archaeological artefacts has been based on the principles of compatibility and minimal intervention. This involves a series of partially unsolved problems, concerning the products used for deteriorated structures consolidation. The choice of materials depends on several factors such as: microclimatic conditions, application methods, and reaction time of products. Recently the employment of nanolime in the consolidation treatments of decorative carbonate matrix surfaces had a great development, thanks to multifunctional use in calcium standard‐sized particles treatments. However, while the use of the nanostructured materials is described in several specialized papers, the information about the best conditions of applicability of the nanolime and its related potentiality for the consolidation in hypogeum environment is rarely considered. The present work is devoted to represent a case study with the aim to give useful elements in order to evaluate the application of nanolime. The funerary inscriptions coming from St. Callixtus Catacombs have been the object of the research carried out in situ and in laboratory, checking indirectly in the short run and in the long run the porosity variation in the materials. The present study intends to indicate the best suspension concentration on consolidation in relationship with hypogeum environment.
Development of Hybrid Braided Composite Rods for Reinforcement and Health Monitoring of Structures
Authors: Rana Sohel , Zdraveva Emilija , Pereira Cristiana , Fangueiro Raul , Correia A. Gomes
Published: January 2014

In the present study, core-reinforced braided composite rods (BCRs) were developed and characterized for strain sensing capability. A mixture of carbon and glass fibre was used in the core, which was surrounded by a braided cover of polyester fibres. Three compositions of core with different carbon fibre/glass fibre weight ratios (23/77, 47/53, and 100/0) were studied to find out the optimum composition for both strain sensitivity and mechanical performance. The influence of carbon fibre positioning in BCR cross-section on the strain sensing behaviour was also investigated. Strain sensing property of BCRs was characterized by measuring the change in electrical resistance with flexural strain. It was observed that BCRs exhibited increase (positive response) or decrease (negative response) in electrical resistance depending on carbon fibre positioning. The BCR with lowest amount of carbon fibre was found to give the best strain sensitivity as well as the highest tensile strength and breaking extension. The developed BCRs showed reversible strain sensing behaviour under cyclic flexural loading with a maximum gauge factor of 23.4 at very low strain level (0.55%). Concrete beams reinforced with the optimum BCR (23/77) also exhibited strain sensing under cyclic flexural strain, although the piezoresistive behaviour in this case was irreversible.
An Investigation into the Performance of Particle Swarm Optimization with Various Chaotic Maps
Authors: Arasomwan Akugbe Martins , Adewumi Aderemi Oluyinka
Published: January 2014

This paper experimentally investigates the effect of nine chaotic maps on the performance of two Particle Swarm Optimization (PSO) variants, namely, Random Inertia Weight PSO (RIW‐PSO) and Linear Decreasing Inertia Weight PSO (LDIW‐PSO) algorithms. The applications of logistic chaotic map by researchers to these variants have led to Chaotic Random Inertia Weight PSO (CRIW‐PSO) and Chaotic Linear Decreasing Inertia Weight PSO (CDIW‐PSO) with improved optimizing capability due to better global search mobility. However, there are many other chaotic maps in literature which could perhaps enhance the performances of RIW‐PSO and LDIW‐PSO more than logistic map. Some benchmark mathematical problems well‐studied in literature were used to verify the performances of RIW‐PSO and LDIW‐PSO variants using the nine chaotic maps in comparison with logistic chaotic map. Results show that the performances of these two variants were improved more by many of the chaotic maps than by logistic map in many of the test problems. The best performance, in terms of function evaluations, was obtained by the two variants using Intermittency chaotic map. Results in this paper provide a platform for informative decision making when selecting chaotic maps to be used in the inertia weight formula of LDIW‐PSO and RIW‐PSO.
Neutrino Oscillations
Authors: Bellini G. , Ludhova L. , Ranucci G. , Villante F. L.
Published: January 2014

In the last decades, a very important breakthrough has been brought about in the elementary particle physics by the discovery of the phenomenon of the neutrino oscillations, which has shown neutrino properties beyond the Standard Model. But a full understanding of the various aspects of the neutrino oscillations is far to be achieved. In this paper the theoretical background of the neutrino oscillation phenomenon is described, referring in particular to the paradigmatic models. Then the various techniques and detectors which studied neutrinos from different sources are discussed, starting from the pioneering ones up to the detectors still in operation and to those in preparation. The physics results are finally presented adopting the same research path which has been crossed by this long saga. The problems not yet fixed in this field are discussed, together with the perspectives of their solutions in the near future.
Micromelanomas: A Review of Melanomas≤2 mm and a Case Report
Authors: Paul Sharad P.
Published: January 2014

The ABCD acronym used to screen pigmented lesions for melanoma obviously was not designed to contend with melanomas that are under 2 mm in diameter. Previously, views ranged that such small lesions could not be melanomas until a few reports of such “micromelanomas” emerged. The author presents a 2 mm melanoma in situ presenting as an insignificant pigmented lesion in a 60-year-old patient with no previous history of melanoma or multiple nevi—which is usually the norm in cases of small melanoma. This paper reiterates the fact that when it comes to a melanoma, size does not matter. In this paper, the term “micromelanoma” is used by the author to represent melanomas under 2 mm. Dermatoscopy and histopathology findings are discussed in this case, along with a review of small melanomas.
In VitroConservation of Sweet Potato Genotypes
Authors: Arrigoni-Blank Maria de Fátima , Tavares Fernanda Ferreira , Blank Arie Fitzgerald , Santos Maria Clézia dos , Menezes Thays Saynara Alves , Santana Aléa Dayane Dantas de
Published: January 2014

The aim of this study was to develop a protocol for thein vitroconservation of sweet potato genotypes using the slow growth technique. The first experiment was conducted in a4×5×2factorial scheme, testing four genotypes (IPB-007, IPB-052, IPB-072, and IPB-137), five concentrations of abscisic acid (ABA) (0.0, 1.0, 2.0, 4.0, and 8.0 mg·L−1), and two temperatures (18 and 25°C). The second experiment was conducted in a4×3×3factorial scheme at 18°C, testing four genotypes (IPB-007, IPB-052, IPB-072, and IPB-137), three variations of MS salts (50, 75, and 100%), and three concentrations of sucrose (10, 20, and 30 g·L−1). Every three months, we evaluated the survival (%), shoot height, and shoot viability.In vitroconservation of the sweet potato genotypes IPB-052 and IPB-007 was obtained over three and six months, respectively, using MS medium plus 2.0 mg·L−1of ABA at either 18 or 25°C. Genotypes IPB-072 and IPB-137 can be kept for three and six months, respectively, in MS medium without ABA at 18°C. It is possible to store IPB-052 and IPB-072 for six months and IPB-007 and IPB-137 for nine months using 30 g·L−1of sucrose and 50% MS salts.
Antimicrobial Activity of Artemisinin and Precursor Derived fromIn VitroPlantlets ofArtemisia annuaL.
Authors: Appalasamy Suganthi , Lo Kiah Yann , Ch'ng Song Jin , Nornadia Ku , Othman Ahmad Sofiman , Chan Lai-Keng
Published: January 2014

Artemisia annuaL., a medicinal herb, produces secondary metabolites with antimicrobial property. In Malaysia due to the tropical hot climate,A. annuacould not be planted for production of artemisinin, the main bioactive compound. In this study, the leaves of threein vitro A. annuaL. clones were, extracted and two bioactive compounds, artemisinin and a precursor, were isolated by thin layer chromatography. These compounds were found to be effective in inhibiting the growth of Gram-positive and Gram-negative bacteria but notCandida albicans. Their antimicrobial activity was similar to that of antibactericidal antibiotic streptomycin. They were found to inhibit the growth of the tested microbes at the minimum inhibition concentration of 0.09 mg/mL, and toxicity test using brine shrimp showed that even the low concentration of 0.09 mg/mL was very lethal towards the brine shrimps with 100% mortality rate. This study hence indicated thatin vitrocultured plantlets ofA. annuacan be used as the alternative method for production of artemisinin and its precursor with antimicrobial activities.
On Angular Limits of Normal Meromorphic Functions: A Geometric Aspect
Authors: Pavicevic Zarko
Published: January 2014

We will prove the assertions which give necessary and sufficient conditions for a normal meromorphic function on the open unit disk to have an angular limit. The results obtained show that the conditions from the classical Lindelöf theorem, as well as the theorems of Lehto and Virtanen and Bagemihl and Seidel, concerning angular limit values of meromorphic functions, can be weakened.
The Preoperative Maximum Standardized Uptake Value Measured by 18F-FDG PET/CT as an Independent Prognostic Factor of Overall Survival in Endometrial Cancer Patients
Authors: Walentowicz-Sadlecka Malgorzata , Malkowski Bogdan , Walentowicz Pawel , Sadlecki Pawel , Marszalek Andrzej , Pietrzak Tomasz , Grabiec Marek
Published: January 2014

Purpose. The aim of this study was to determine if the preoperative maximum standardized uptake value (SUVmax) measured by 18F-FDG PET/CT in the primary tumor has prognostic value in the group of patients with endometrial cancer.Patients, Materials, and Methods. A total of one hundred one consecutive endometrial cancer patients, age range 40–82 years (mean 62 years) and FIGO I–IV stage, who underwent 18-FDG-PET/CT within two weeks prior radical surgery, were enrolled to the study. The maximum SUV was measured and compared with the clinicopathologic features of surgical specimens. The relationship between SUVmax and overall survival was analyzed.Results. The mean preoperative SUVmax was 14.34; range (3.90–33.80) and was significantly lower for FIGO I than for higher stages (P=0.0012), as well as for grade 1 than for grade 2 and 3 (P=0.018), deep myometrial invasion (P=0.0016) and for high risk group (P=0.0004). The analysis of survival ROC curve revealed SUVmax cut-off value of 17.7 to predict high risk of recurrence. Endometrial cancer patients with SUVmax higher than 17.7 characterized by lower overall survival.Conclusion. The preoperative SUVmax measured by 18F-FDG PET/CT is considered as an important indicator reflecting tumor aggressiveness which may predict poor prognosis. High value of SUVmax would be useful for making noninvasive diagnoses and deciding the appropriate therapeutic strategy for patients with endometrial cancer.
Construction of a Novel Liver-Targeting Fusion Interferon by Incorporation of aPlasmodiumRegion I-Plus Peptide
Authors: Lu Xuemei , Jin Xiaobao , Huang Yanting , Wang Jie , Shen Juan , Chu Fujiang , Mei Hanfang , Ma Yan , Zhu Jiayong
Published: January 2014

Interferon alpha (IFNα) exerts a multiplicity of biological actions including antiviral, immunomodulatory, and antiproliferative effects. Administration of IFNαis the current treatment for chronic hepatitis B; however, therapy outcome has not been completely satisfactory. The systemic effects of IFNαmay account for its lowin vivobiological activity and multiple adverse events. The purpose of this study was to design a novel liver-targeting fusion interferon (IFN-CSP) by fusing IFNα2b with aPlasmodiumregion I-plus peptide, thus targeting the drug specifically to the liver. The DNA sequence encoding IFN-CSP was constructed using improved splicing by overlapping extension-PCR method, and then cloned into the pET-21b vector for protein expression inE. coliBL21 (DE3). The recombinant protein was expressed as a His-tagged protein and purified using a combination of Ni affinity and HiTrap affinity chromatography at a purity of over 95%. The final yield of biologically active IFN-CSP was up to 270 mg/L culture. The purified recombinant protein showed anti-HBV activity and liver-targeting potentialityin vitro. These data suggests that the novel fusion interferon IFN-CSP may be an excellent candidate as a liver-targeting anti-HBV agent.
Pain Management in the Elderly: Transdermal Fentanyl for the Treatment of Pain Caused by Osteoarthritis of the Knee and Hip
Authors: Mordarski Sylwester
Published: January 2014

This study was designed to evaluate the utility of transdermal fentanyl (transdermal fentanyl, TDF) for the treatment of pain due to osteoarthritis (osteoarthritis, OA) of the knee and hip, which was not adequately controlled by nonopioid analgesics or weak opioids. WOMAC is a reliable, valid, and responsive multidimensional, self-administrated outcome measure designed specifically to evaluate patients with OA of the knee or hip. TDF significantly increased pain control and improved functioning and quality of life. Metoclopramide appeared to be of limited value in preventing nausea and vomiting.
Synchronized Analysis of FTIR Spectra and GCMS Chromatograms for Evaluation of the Thermally Degraded Vegetable Oils
Authors: Sim Siong Fong , Lee Terri Zhuan Ean , Mohd Irwan Lu Nurul Aida Lu , Samling Benedict
Published: January 2014

Fourier Transform Infrared (FTIR) and Gas Chromatography Mass Spectrometry (GCMS) are two common instruments used for analysis of edible oils. The output signal is often analysed on the software attached to the workstations. The processing software is usually individualised for a specific source. The output of GCMS cannot be analysed on the FTIR hence analysts often need to juggle between instruments when multiple techniques are employed. This could become exhaustive when a large dataset is involved. This paper reports a synchronised approach for analysis of signal from FTIR and GCMS. The algorithm is demonstrated on a dataset of edible oils to investigate the thermal degradation of seven types of edible oils treated at 100°C and 150°C. The synchronised routines identify peaks present in FTIR and GCMS spectra/chromatograms where the information is subsequently extracted onto peak tables for further analysis. In this study, it is found that palm based products and corn oils were relatively more stable with higher content of antioxidants tocopherols and squalene. As a conclusion, this approach allows simultaneous analysis of signal from multiple sources and samples enhancing the efficiency of the signal processing process.
On Generalization Based on Bi et al. Iterative Methods with Eighth-Order Convergence for Solving Nonlinear Equations
Authors: Lotfi Taher , Cordero Alicia , Torregrosa Juan R. , Amir Abadi Morteza , Mohammadi Zadeh Maryam
Published: January 2014

The primary goal of this work is to provide a general optimal three-step class of iterative methods based on the schemes designed by Bi et al. (2009). Accordingly, it requires four functional evaluations per iteration with eighth-order convergence. Consequently, it satisfies Kung and Traub’s conjecture relevant to construction optimal methods without memory. Moreover, some concrete methods of this class are shown and implemented numerically, showing their applicability and efficiency.
Antioedematous and Analgesic Properties of Fertile Fronds of Drynaria quercifolia
Authors: Anuja G. I. , Latha P. G. , Shine V. J. , Suja S. R. , Shikha P. , Satheesh Kumar K. , Rajasekharan S.
Published: January 2014

Inflammation is a complex biological response of tissue cells to harmful stimuli including trauma, tissue necrosis, and infections which plays a key role in the pathophysiology of many deadly diseases. In ethnomedicine Drynaria quercifolia fronds are used to treat inflammation as poultice on swellings and as antibacterial, hepatoprotective, and antipyretic agent. Herein, we have evaluated the antioedematous, antiproliferative, and analgesic properties of the ethanolic extract of fertile fronds of D. quercifolia (FF) by standard procedures. Oral administration of FF produced significant inhibition of carrageenan and histamine induced paw oedema in Wistar rats. FF significantly reduced both wet weight and dry weight of granuloma tissue which shows the inhibitory effect on exudative and proliferative phases of inflammation. FF significantly attenuated acute and delayed phases of formalin induced pain, acetic acid-induced writhing, capsaicin-induced nociception, and hot plate test in mice. Phytochemical analysis revealed the presence of coumarins, flavonoids, glycosides, phenolics, saponins, steroids, tannins, and terpenoids. Total phenolic content was 186 mg/g equivalent of gallic acid. The HPLC estimation showed flavanone glycoside naringin (1.2%) and its aglycone naringenin (0.02%). The presence of potent anti-inflammatory and analgesic principles in FF and their synergistic action may be the reason for the proposed therapeutic effects.
Search for the Anomalous Interactions of Up-Type Heavy Quarks inγγCollision at the LHC
Authors: Köksal M. , İnan S. C.
Published: January 2014

We investigate the anomalous interactions of heavy up-type quarkt′in aγγcollision at the LHC. We have obtained 95% confidence level (CL) limit oft′qγ(q=u,c) anomalous coupling by taking into account three forward detector acceptances:0.0015<ξ<0.15,0.0015<ξ<0.5, and0.1<ξ<0.5.
X-Ray of One-Sided “White Lung” after Central Venous Catheterization
Authors: Casanova Michel , Ummenhofer Wolfgang
Published: January 2014

Complications during insertion of a subclavian central venous line are rare but potentially serious. This case report describes the radiological abnormality of a one-sided pleural effusion during a routine control directly after a difficult central venous catheterization. We illustrate the findings, the initial emergency management, and our procedure to rule out an iatrogenic hemothorax. Possible differential diagnoses and strategies for management of a suspected complication are discussed.
Modified Projection Algorithms for Solving the Split Equality Problems
Authors: Dong Qiao-Li , He Songnian
Published: January 2014

The split equality problem (SEP) has extraordinary utility and broad applicability in many areas of applied mathematics. Recently, Byrne and Moudafi (2013) proposed a CQ algorithm for solving it. In this paper, we propose a modification for the CQ algorithm, which computes the stepsize adaptively and performs an additional projection step onto two half-spaces in each iteration. We further propose a relaxation scheme for the self-adaptive projection algorithm by using projections onto half-spaces instead of those onto the original convex sets, which is much more practical. Weak convergence results for both algorithms are analyzed.
In SituPreparation of Polyether Amine Functionalized MWCNT Nanofiller as Reinforcing Agents
Authors: Yıldrım Ayber , Seçkin Turgay
Published: January 2014

In situpreparation of polyether amine functionalized cross-linked multiwalled carbon nanotube (MWCNT) nanofillers may improve the thermal and mechanical properties of the composites in which they are used as reinforcing agents. The reduction and functionalization of MWCNT using ethylenediamine in the presence of polyether amine produced stitched MWCNT's due to the presence of two amine (–NH2) functionalities on both sides of the polymer. Polyether amine was chosen to polymerize the carboxylated MWCNT due to its potential to form bonds with the amino groups and carboxyl groups of MWCNT which produces a resin used as polymeric matrix for nanocomposite materials. The attachment of the polyether amine (Jeffamine) groups was verified by TGA, FT-IR, XRD, SEM, and Raman spectroscopy. The temperature at which the curing enthalpy is maximum, observed by DSC, was shifted to higher values by adding functionalized MWCNT. SEM images show the polymer formation between MWCNT sheets.
Routine Follow-Up Cranial Computed Tomography for Deeply Sedated, Intubated, and Ventilated Multiple Trauma Patients with Suspected Severe Head Injury
Authors: Wurmb Thomas Erik , Schlereth Stefan , Kredel Markus , Muellenbach Ralf M. , Wunder Christian , Brederlau Jörg , Roewer Norbert , Kenn Werner , Kunze Ekkehard
Published: January 2014

Background. Missed or delayed detection of progressive neuronal damage after traumatic brain injury (TBI) may have negative impact on the outcome. We investigated whether routine follow-up CT is beneficial in sedated and mechanically ventilated trauma patients.Methods. The study design is a retrospective chart review. A routine follow-up cCT was performed 6 hours after the admission scan. We defined 2 groups of patients, group I: patients with equal or recurrent pathologies and group II: patients with new findings or progression of known pathologies.Results. A progression of intracranial injury was found in 63 patients (42%) and 18 patients (12%) had new findings in cCT 2 (group II). In group II a change in therapy was found in 44 out of 81 patients (54%). 55 patients with progression or new findings on the second cCT had no clinical signs of neurological deterioration. Of those 24 patients (44%) had therapeutic consequences due to the results of the follow-up cCT.Conclusion. We found new diagnosis or progression of intracranial pathology in 54% of the patients. In 54% of patients with new findings and progression of pathology, therapy was changed due to the results of follow-up cCT. In trauma patients who are sedated and ventilated for different reasons a routine follow-up CT is beneficial.
Matrix Effects on the Stability and Antioxidant Activity of Red Cabbage Anthocyanins under Simulated Gastrointestinal Digestion
Authors: Podsędek Anna , Redzynia Małgorzata , Klewicka Elżbieta , Koziołkiewicz Maria
Published: January 2014

Red cabbage is, among different vegetables, one of the major sources of anthocyanins. In the present study an in vitro digestion method has been used to assay the influence of the physiological conditions in the stomach and small intestine, as well as faecal microflora on anthocyanins stability in red cabbage and anthocyanin-rich extract. The recovery of anthocyanins during in vitro gastrointestinal digestion was strongly influenced by food matrix. The results showed that other constituents present in cabbage enhanced the stability of anthocyanins during the digestion. The amount of anthocyanins (HPLC method) and antioxidant capacity (ABTS and FRAP assays) strongly decreased after pancreatic-bile digestion in both matrices but total phenolics content (Folin-Ciocalteu assay) in these digestions was higher than in initial samples. Incubation with human faecal microflora caused further decline in anthocyanins content. The results obtained suggest that intact anthocyanins in gastric and products of their decomposition in small and large intestine may be mainly responsible for the antioxidant activity and other physiological effects after consumption of red cabbage.
Notch1 and 4 Signaling Responds to an Increasing Vascular Wall Shear Stress in a Rat Model of Arteriovenous Malformations
Authors: Tu Jian , Li Yang , Hu Zhiqiang
Published: January 2014

Notch signaling is suggested to promote the development and maintenance of cerebral arteriovenous malformations (AVMs), and an increasing wall shear stress (WSS) contributes to AVM rupture. Little is known about whether WSS impacts Notch signaling, which is important for understanding the angiogenesis of AVMs. WSS was measured in arteriovenous fistulas (AVF) surgically created in 96 rats at different time points over a period of 84 days. The expression of Notch receptors 1 and 4 and their ligands, Delta1 and 4, Jagged1, and Notch downstream gene target Hes1 was quantified in “nidus” vessels. The interaction events between Notch receptors and their ligands were quantified using proximity ligation assay. There was a positive correlation between WSS and time (r=0.97;P<0.001). The expression of Notch receptors and their ligands was upregulated following AVF formation. There was a positive correlation between time and the number of interactions between Notch receptors and their ligands aftre AVF formation (r=0.62,P<0.05) and a positive correlation between WSS and the number of interactions between Notch receptors and their ligands (r=0.87,P<0.005). In conclusion, an increasing WSS may contribute to the angiogenesis of AVMs by activation of Notch signaling.
Direct Cell-Cell Contact between Mesenchymal Stem Cells and Endothelial Progenitor Cells Induces a Pericyte-Like Phenotype In Vitro
Authors: Loibl Markus , Binder Andreas , Herrmann Marietta , Duttenhoefer Fabian , Richards R. Geoff , Nerlich Michael , Alini Mauro , Verrier Sophie
Published: January 2014

Tissue engineering techniques for the regeneration of large bone defects require sufficient vascularisation of the applied constructs to ensure a sufficient supply of oxygen and nutrients. In our previous work, prevascularised 3D scaffolds have been successfully established by coculture of bone marrow derived stem cells (MSCs) and endothelial progenitor cells (EPCs). We identified stabilising pericytes (PCs) as part of newly formed capillary-like structures. In the present study, we report preliminary data on the interactions between MSCs and EPCs, leading to the differentiation of pericyte-like cells. MSCs and EPCs were seeded in transwell cultures, direct cocultures, and single cultures. Cells were cultured for 10 days in IMDM 10% FCS or IMDM 5% FCS 5% platelet lysate medium. Gene expression of PC markers, CD146, NG2,αSMA, and PDGFR-β, was analysed using RT-PCR at days 0, 3, 7, and 10. The upregulation of CD146, NG2, andαSMA in MSCs in direct coculture with EPCs advocates the MSCs’ differentiation towards a pericyte-like phenotype in vitro. These results suggest that pericyte-like cells derive from MSCs and that cell-cell contact with EPCs is an important factor for this differentiation process. These findings emphasise the concept of coculture strategies to promote angiogenesis for cell-based tissue engineered bone grafts.
Standardization ofTragopogon graminifoliusDC. Extract Based on Phenolic Compounds and Antioxidant Activity
Authors: Farzaei Mohammad Hosein , Khanavi Mahnaz , Moghaddam Ghazaleh , Dolatshahi Farzaneh , Rahimi Roja , Shams-Ardekani Mohammad Reza , Amin Gholamreza , Hajimahmoodi Mannan
Published: January 2014

Tragopogon graminifoliusDC. (TG), Compositae family, is traditionally used for the treatment of various diseases like gastrointestinal and hepatic disorders. The aim of the present study is to standardize extracts from TG used for preparation of different dosage forms in traditional Iranian medicine (TIM) based on phenolic compounds. For this purpose, total phenolic content and some phenolic compounds were determined in ethanolic extracts from aerial part and root of TG by HPLC method. Furthermore, antioxidant activity was evaluated using DPPH-HPLC methods. Caffeic acid, gallic acid,ρ-coumaric acid, ferulic acid, and catechin were detected in root and aerial part of TG.ρ-Coumaric acid (6.357 ± 0.014 mg·g−1) was dominant phenolic compound in aerial part followed by ferulic acid (1.24 ± 0.018 mg·g−1). Also,ρ-coumaric acid (2.685 ± 0.031 mg·g−1) was highly abundant in root, followed by catechin (2.067 ± 0.021 mg·g−1). Antioxidant activity of root extract (460.45 ± 0.78 µg Vit.E.E·mL−1) was better than that of aerial part. Generally, phenolic compounds are one of the major constituents of TG and could be used as markers for standardization of dosage forms prepared from this plant. Also, TG demonstrated significant antioxidant activity using DPPH-HPLC method. Phenolic compounds of TG may be responsible for its marked antioxidant properties.
Nonalcoholic Wernicke’s Encephalopathy Associated with Unintentional Weight Loss, Cholecystectomy, and Intractable Vomiting: The Role of Dual Thiamine and Corticosteroid Therapy
Authors: Verma Vivek , Donadee Chenell , Gomez Leslie , Zaretskaya Marina
Published: January 2014

A 23-year-old male with one month of intractable vomiting, subsequent cholecystitis status post cholecystectomy, and overall 40-pound weight loss over the last few months presented with altered mental status and seizures. MRI showed signal abnormalities involving the hypothalamus, periaqueductal gray matter, 4th ventricle, and bilateral thalami, indicative of Wernicke’s encephalopathy. The patient was started on empiric IV thiamine and methylprednisolone; thiamine levels were subsequently found to be low. Infectious disease workup was negative. Within a few days of this therapy, the patient’s neurological status steadily improved with increased responsiveness and communication. Repeat MRI 7 days after admission showed significant resolution of the signal abnormalities. Over the next several weeks the patient became fully conversational, cognitively intact, and increasingly ambulatory. Nonalcoholic Wernicke’s encephalopathy is rare; there have been reports relating it separately to vomiting and invasive surgery. In this case report, we associate it with both recurrent vomiting and minimally invasive cholecystectomy. We also discuss combinatorial therapy of thiamine and corticosteroids, which is poorly defined in the literature. Though there is no consensus-based optimal treatment of Wernicke’s encephalopathy, this adds to the discussion of using dual therapy and supports that the use of empiric corticosteroids does not harm the patient.