bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Flexible Algorithm for the Optimization of Slot Filling Factors in Electrical Machines
Authors: Dietz Armin , Di Tommaso Antonino Oscar , Marignetti Fabrizio , Miceli Rosario , Nevoloso Claudio
Published: February 2020

The continuous development in the field of industrial automation and electric mobility has led to the need for more efficient electrical machines with a high power density. The improvement of electrical machines’ slot filling factors is one of the measures to satisfy these requirements. In recent years, this topic has aroused greater interest in the industrial sector, since the evolution of the winding technological manufacturing processes allows an economically sustainable realization of ordered winding arrangements, rather than random ones. Moreover, the manufacture of electrical machines’ windings must be preceded by an accurate design phase in which it is possible to evaluate the maximum slot filling factor obtainable for a given wire shape and for its dimensions. For this purpose, this paper presents an algorithmic approach for the evaluation of maximum slot filling factors in electrical machines under an ideal geometric premise. In particular, this algorithm has a greater degree of flexibility with respect to the algorithm approaches found in the literature, since the study has been extended to round, rectangular and hexagonal wire sections. Furthermore, the slot filling factor calculation was carried out both for standard and non-standard slots. The algorithmic approach proposed can be considered as an additional useful tool for the fast design of electrical machine windings.
Regulated Emissions of Biogas Engines—On Site Experimental Measurements and Damage Assessment on Human Health
Authors: Macor Alarico , Benato Alberto
Published: February 2020

Despite biogas renewability, it is mandatory to experimentally assess its combustion products in order to measure their pollutants content. To this purpose, the Authors selected six in-operation biogas plants fed by different substrates and perform an on-site experimental campaign for measuring both biogas and engines exhausts composition. Firstly, biogas measured compositions are compared among them and with data available in literature. Then, biogas engines’ exhaust compositions are compared among them, with data available in literature and with measurements obtained from an engine characterised by the same design power but fuelled with natural gas. Finally, the Health Impact Assessment analysis is used to estimate the damage on human health caused by both biogas and natural gas engines emissions. Results show that biogas causes a damage on human health three times higher than the natural gas one. But, this approach does not consider biogas renewability. So, to include this important aspect, also an analysis which considers Global Warming categories is carried out. Results highlight that natural gas is twice harmful than biogas.
A Multicriteria Methodology to Select the Best Installation of Solar Thermal Power in a Family House
Authors: Košičan Jaroslav , Pardo Miguel Ángel , Vilčeková Silvia
Published: February 2020

Solar thermal power is nowadays one of the trendiest topics in the construction industry, and it represents a valuable energy source of heating that reduces energy consumption. As solar panels produce heating during the day and consumers demand calefaction during the whole day, we use standby tanks (for domestic hot water) and buffer tanks (for heating) for storage. The latest developments improved the efficiency and useful life while reducing the volume of tanks. So, the presented research work deals with analyzing the solar thermal power in a family house. This work presents a method to create a decision support system to compare solar energy systems in houses from economical, technical, availability, and environmental concerns. The weights of the criteria selected considering the analytical hierarchy process are computed. Parameters required for energy production calculations (location, temperature, etc.) and energy consumption (inhabitants, outdoor temperature, etc.) are summarized. It can be stated that a universal best solar thermal scheme does not exist, as energy consumption depends on the other features and limits as well as energy production, geographical latitude of the location, and so forth. According to results, Case 3 (a gas boiler and a combination tank) is the best alternative for reducing the energy required, CO2 emitted, the best energy efficiency of the installation, and the lowest transmission losses. In other scenarios when the economic criteria are not so relevant, this should be the best case in the prioritization scheme.
Lignocellulosic Ethanol in a Greenhouse Gas Emission Reduction Obligation System—A Case Study of Swedish Sawdust Based-Ethanol Production
Authors: Haus Sylvia , Björnsson Lovisa , Börjesson Pål
Published: February 2020

A greenhouse gas (GHG) emission reduction obligation system has been implemented in the Swedish road transport sector to promote the use of biofuels. For transportation fuel suppliers to fulfil this obligation, the volume of biofuel required decreases with decreasing life cycle GHG emission for the biofuel, linking lower GHG emission to higher economic value. The aim of this study was to investigate how the economic competitiveness of a Swedish emerging lignocellulosic-based ethanol production system would be influenced by the reduction obligation. The life cycle GHG emission for sawdust-based ethanol was calculated by applying the method advocated in the EU Renewable Energy Directive (RED II). The saving in GHG emissions, compared with fossil liquid transportation fuels, was 93% for a potential commercial production system in southern Sweden. This, in turn, will increase the competitiveness of sawdust-based ethanol compared to the mainly crop-based ethanol currently used in the Swedish biofuel system, which has an average GHG emission saving of 68%, and will allow for an almost 40% higher price of sawdust-based ethanol, compared to the current price of ethanol at point of import. In a future developed, large-scale market of advanced ethanol, today’s GHG emission reduction obligation system in Sweden seems to afford sufficient economic advantage to make lignocellulosic ethanol economically viable. However, in a short-term perspective, emerging lignocellulosic-based ethanol production systems are burdened with economic risks and therefore need additional economic incentives to make a market introduction possible.
Comparative Analysis of Energy Consumption between Green Building Certified and Non-Certified Buildings in Korea
Authors: No Sangtae , Won Chungyeon
Published: February 2020

Although interest in and the importance of green building certification have increased, it is difficult to determine how much less energy the buildings actually consume after obtaining sustainability related certification, such as LEED in the USA, and G-SEED in Korea. Focusing on building energy evaluation, we compared and analyzed the energy evaluation items of LEED and G-SEED. In addition, from the Korean public data portal site, this study investigated the annual site energy consumption (electricity and gas) per floor area of non-residential buildings that obtained G-SEED certification in Korea. The energy consumption of non-certified general buildings was compared with the energy consumption of certified buildings. As a result of examining 84 samples of this study, non-residential buildings with G-SEED certification showed energy use intensity that was at least (35.5 to 48.9)% lower than that of uncertified buildings.
Carrier-Phase-Shifted Rotation Pulse-Width-Modulation Scheme for Dynamic Active Power Balance of Modules in Cascaded H-Bridge STATCOMs
Authors: Xu Chen , Chen Jingjing , Dai Ke
Published: February 2020

In the medium-voltage power distribution system, cascaded H-bridge (CHB) static synchronous compensators (STATCOMs) are widely utilized to solve power quality issues by injecting the controlled reactive current into the system. The carrier-phase-shifted (CPS) pulse-width-modulation (PWM) scheme is preferred for CHB-STATCOMs, because it can minimize the compensating current distortion and realize the relative active power balance among the H-bridge modules. This paper reveals the influence of the carrier phase difference on the module active power balance, and proposes a carrier rotation technique with a CPS-PWM scheme to address this drawback. The rotation rules were analyzed, and the rotation time was especially designed to enhance the robustness of the system. With the proposed method, the natural dynamic active power balance of each module could be achieved, and the capacitor voltage could maintain balance without the individual capacitor voltage control or the auxiliary circuits in theory. The experimental results acquired from a downscaled CHB-STATCOM prototype demonstrated the feasibility of the proposed CPS rotation PWM scheme.
Public-Private or Master-Servant? Examining the Implementation of the Serious Disease Insurance Scheme in China
Authors: Ma Yingying , Liu Zhuojun , Shen Shuguang
Published: February 2020

China’s Serious Disease Insurance Scheme (SDIS) was set up to relieve the financial burdens on serious disease patients. It is a crucial part of the national basic medical insurance scheme, which is regarded as one of the largest government-funded social security programs in the world. The most significant institutional innovation of the SDIS is that the approach of a public–private partnership (PPP) is applied in an attempt to facilitate the efficiency of its implementation. The objective of this paper is to evaluate the implementation of the SDIS in China through PPPs, and to identify the problems to be tackled if the Chinese government intends to make such a plan work better for the majority of urban and rural residents. With the effective support from local officials and practitioners, the authors of this paper collected copies of SDIS contracts of multiple cities in Guangdong, one of the most developed provinces of China. Guided by a research framework drawn from the PPP literature, details of contract enforcement were also examined. The authors discovered that the role of local states is rather dominant; they have manipulated contract drafting and implementation. Additionally, current mechanisms for profit sharing, risk sharing, and information exchange have placed insurance companies in a rather disadvantageous situation. To achieve the sustainable development of the SDIS, the authors suggest that a further reform on implementation of a PPP must be pushed forward.
Understanding the Experiences and Needs of Migrant Women Affected by Female Genital Mutilation Using Maternity Services in Australia
Authors: Turkmani Sabera , Homer Caroline , Dawson Angela
Published: February 2020

Female genital mutilation (FGM) is a cultural practice defined as the partial or total removal of the external female genitalia for non-therapeutic reasons. Changing patterns of migration in Australia and other high-income countries has meant that maternity care providers and health systems are caring for more pregnant women affected by this practice. The aim of the study was to identify strategies to inform culturally safe and quality woman-centred maternity care for women affected by FGM who have migrated to Australia. An Appreciative Inquiry approach was used to engage women with FGM. We conducted 23 semi-structured interviews and three focus group discussions. There were four themes identified: (1) appreciating the best in their experiences; (2) achieving their dreams; (3) planning together; and (4) acting, modifying, improving and sustaining. Women could articulate their health and cultural needs, but they were not engaged in all aspects of their maternity care or considered active partners. Partnering and involving women in the design and delivery of their maternity care would improve quality care. A conceptual model, underpinned by women’s cultural values and physical, emotional needs, is presented as a framework to guide maternity services.
Hyperlipidemia and Statins Use for the Risk of New Diagnosed Sarcopenia in Patients with Chronic Kidney: A Population-Based Study
Authors: Lin Min-Hua , Chiu She-Yu , Chang Pei-Hsuan , Lai Yu-Liang , Chen Pau-Chung , Ho Wen-Chao
Published: February 2020

Background: Previous research found that statins, in addition to its efficiency in treating hyperlipidemia, may also incur adverse drug reactions, which mainly include myopathies and abnormalities in liver function. Aim: This study aims to assess the risk for newly onset sarcopenia among patients with chronic kidney disease using statins. Material and Method: In a nationwide retrospective population-based cohort study, 75,637 clinically confirmed cases of chronic kidney disease between 1997 and 2011were selected from the National Health Insurance Research Database of Taiwan. The selection of the chronic kidney disease cohort included a discharge diagnosis with chronic kidney disease or more than 3 outpatient visits with the diagnosis of chronic kidney disease found within 1 year. After consideration of patient exclusions, we finally got a total number of 67,001 cases of chronic kidney disease in the study. The Cox proportional hazards model was used to perform preliminary analysis on the effect of statins usage on the occurrence of newly diagnosed sarcopenia; the Cox proportional hazards model with time-dependent covariates was conducted to take into consideration the individual temporal differences in medication usage, and calculated the hazard ratio (HR) and 95% confidence interval after controlling for gender, age, income, and urbanization. Results: Our main findings indicated that patients with chronic kidney disease who use statins seem to effectively prevent patients from occurrences of sarcopenia, high dosage of statins seem to show more significant protective effects, and the results are similar over long-term follow-up. In addition, the risk for newly diagnosed sarcopenia among patients with lipophilic statins treatment was lower than that among patients with hydrophilic statins treatment. Conclusion: It seems that patients with chronic kidney disease could receive statin treatment to reduce the occurrence of newly diagnosed sarcopenia. Additionally, a higher dosage of statins could reduce the incidence of newly diagnosed sarcopenia in patients with chronic kidney disease.
Characterization of Esophageal Cancer and Its Association with Influencing Factors in Guangzhou City, China
Authors: Cui Cheng , Dong Hang , Ren Hongyan , Lin Guozhen , Zhao Lu
Published: February 2020

Epidemiological features of esophageal cancer (EC), as well as their associations with potential influencing factors in a city, have seldom been seldom explored on a fine scale. The EC death cases in Guangzhou city during 2012−2017 were collected to describe the epidemiological characteristics such as EC mortality rate (ECMR) and health-seeking behaviors of deaths. Potential influencing factors, including socioeconomic conditions (population density, gross domestic product density), medical resources, and ageing degree were also gathered for exploring their relationships with the epidemiological characteristics of EC. A total of 2,409 EC deaths were reported during 2012−2017 in Guangzhou with an age-standardized ECMR of 3.18/105. The prevalence of EC in Guangzhou was spatially featured and was divided into three regions with obvious differentiated ECMR (ECMR of 6.41/105 in region A, ECMR of 5.51/105 in region B, ECMR of 2.56/105 in region C). The street/town-level ECMR was spatially clustered in Guangzhou city, especially two clusters of streets/towns with high ECMR were highlighted in region A and B respectively. Meanwhile, demographic features including gender gap, death age, temporal interval between diagnosis and death, health-seeking behaviors were remarkably different among the three regions. Moreover, health-seeking behaviors (e.g., the proportion of hospital deaths) of the EC deaths were obviously influenced by medical institution occupancy rate and socioeconomic conditions at street/town level. In addition, the street/town-level ECMR was significantly associated with ageing degree across Guangzhou city (r = 0.466, p < 0.01), especially in region A (r = 0.565, p < 0.01). In contrast, the ECMR in region B was closely related to population density (r = −0.524, p < 0.01) and gross domestic product density (r = −0.511, p < 0.01) when the ageing degree was controlled, while these associations were weak in region C. The epidemiological characteristics of EC in Guangzhou city were spatially featured and potentially associated with socioeconomic conditions, medical resources and ageing degree on a fine scale across Guangzhou city. This study could provide scientific basis for local authorities to implement more targeted EC interventions.
Health Advertising on Short-Video Social Media: A Study on User Attitudes Based on the Extended Technology Acceptance Model
Authors: Zhao Jie , Wang Jianfei
Published: February 2020

The rapid development of short-video social network platforms provides us with an opportunity to conduct health-related advertising and recommendation. However, so far, there are no empirical evidence on whether users are willing to accept health-related short-video advertisements. Here, acceptance refers to purchase intention, meaning that users will read short-video ads, share ads with others, or even open the product link embedded in ads to purchase the product. In this paper, we make the first attempt to model and quantify user acceptance of health-related short-video advertisements. Particularly, we propose a new research model that enhances the Technology Acceptance Model (TAM) with two new designs. First, we propose four new antecedents including social interaction, intrusiveness, informativeness, and relevance into the original TAM to reflect the features of short-video social networks. Second, we introduce two mediator variables including perceived usefulness and attitude so that we can better study how different factors affect user acceptance of health-related short-video ads. We perform a survey on the Internet and conduct an empirical analysis of the surveyed data. The results show that the four antecedents as well as the perceived ease of use have significant influences on perceived usefulness, attitude, and purchase intention. Further, perceived usefulness plays a valid mediating role in attitude and purchase intention. We also found that users’ perceived ease of use on health-related short-video ads cannot significantly predict users’ attitudes toward ads. This is a new finding in social media-oriented ads. Finally, we integrate the empirical findings and present reasonable suggestions for advertisers and marketers to promote health-related short-video ads.
Loss of Histone Locus Bodies in the Mature Hemocytes of Larval Lymph Gland Result in Hyperplasia of the Tissue in mxc Mutants of Drosophila
Authors: Kurihara Masanori , Komatsu Kouyou , Awane Rie , Inoue Yoshihiro H.
Published: February 2020

Mutations in the multi sex combs (mxc) gene in Drosophila results in malignant hyperplasia in larval hematopoietic tissues, called lymph glands (LG). mxc encodes a component of the histone locus body (HLB) that is essential for cell cycle-dependent transcription and processing of histone mRNAs. The mammalian nuclear protein ataxia-telangiectasia (NPAT) gene, encoded by the responsible gene for ataxia telangiectasia, is a functional Mxc orthologue. However, their roles in tumorigenesis are unclear. Genetic analyses of the mxc mutants and larvae having LG-specific depletion revealed that a reduced activity of the gene resulted in the hyperplasia, which is caused by hyper-proliferation of immature LG cells. The depletion of mxc in mature hemocytes of the LG resulted in the hyperplasia. Furthermore, the inhibition of HLB formation was required for LG hyperplasia. In the mutant larvae, the total mRNA levels of the five canonical histones decreased, and abnormal forms of polyadenylated histone mRNAs, detected rarely in normal larvae, were generated. The ectopic expression of the polyadenylated mRNAs was sufficient for the reproduction of the hyperplasia. The loss of HLB function, especially 3′-end processing of histone mRNAs, is critical for malignant LG hyperplasia in this leukemia model in Drosophila. We propose that mxc is involved in the activation to induce adenosine deaminase-related growth factor A (Adgf-A), which suppresses immature cell proliferation in LG.
Sonic Hedgehog Signaling and Tooth Development
Authors: Hosoya Akihiro , Shalehin Nazmus , Takebe Hiroaki , Shimo Tsuyoshi , Irie Kazuharu
Published: February 2020

Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages. A number of studies have analyzed the function of Shh signaling at different stages of tooth development and have revealed that Shh signaling regulates the formation of various tooth components, including enamel, dentin, cementum, and other soft tissues. In addition, dental mesenchymal cells positive for Gli1, a downstream transcription factor of Shh signaling, have been found to have stem cell properties, including multipotency and the ability to self-renew. Indeed, Gli1-positive cells in mature teeth appear to contribute to the regeneration of dental pulp and periodontal tissues. In this review, we provide an overview of recent advances related to the role of Shh signaling in tooth development, as well as the contribution of this pathway to tooth homeostasis and regeneration.
Genetic Factors of Nitric Oxide’s System in Psychoneurologic Disorders
Authors: Nasyrova Regina F. , Moskaleva Polina V. , Vaiman Elena E. , Shnayder Natalya A. , Blatt Nataliya L. , Rizvanov Albert A.
Published: February 2020

According to the recent data, nitric oxide (NO) is a chemical messenger that mediates functions such as vasodilation and neurotransmission, as well as displaying antimicrobial and antitumoral activities. NO has been implicated in the neurotoxicity associated with stroke and neurodegenerative diseases; neural regulation of smooth muscle, including peristalsis; and penile erections. We searched for full-text English publications from the past 15 years in Pubmed and SNPedia databases using keywords and combined word searches (nitric oxide, single nucleotide variants, single nucleotide polymorphisms, genes). In addition, earlier publications of historical interest were included in the review. In our review, we have summarized information regarding all NOS1, NOS2, NOS3, and NOS1AP single nucleotide variants (SNVs) involved in the development of mental disorders and neurological diseases/conditions. The results of the studies we have discussed in this review are contradictory, which might be due to different designs of the studies, small sample sizes in some of them, and different social and geographical characteristics. However, the contribution of genetic and environmental factors has been understudied, which makes this issue increasingly important for researchers as the understanding of these mechanisms can support a search for new approaches to pathogenetic and disease-modifying treatment.
Monocarboxylate Transporter 1 (MCT1) in Liver Pathology
Authors: Droździk Marek , Szeląg-Pieniek Sylwia , Grzegółkowska Justyna , Łapczuk-Romańska Joanna , Post Mariola , Domagała Pawel , Miętkiewski Janusz , Oswald Stefan , Kurzawski Mateusz
Published: February 2020

Membrane monocarboxylate transporter 1 (SLC16A1/MCT1) plays an important role in hepatocyte homeostasis, as well as drug handling. However, there is no available information about the impact of liver pathology on the transporter levels and function. The study was aimed to quantify SLC16A1 mRNA (qRT-PCR) and MCT1 protein abundance (liquid chromatography–tandem mass spectrometry (LC¬¬–MS/MS)) in the livers of patients diagnosed, according to the standard clinical criteria, with hepatitis C, primary biliary cirrhosis, primary sclerosing hepatitis, alcoholic liver disease (ALD), and autoimmune hepatitis. The stage of liver dysfunction was classified according to Child–Pugh score. Downregulation of SLC16A1/MCT1 levels was observed in all liver pathology states, significantly for ALD. The progression of liver dysfunction, from Child–Pugh class A to C, involved the gradual decline in SLC16A1 mRNA and MCT1 protein abundance, reaching a clinically significant decrease in class C livers. Reduced levels of MCT1 were associated with significant intracellular lactate accumulation. The MCT1 transcript and protein did not demonstrate significant correlations regardless of the liver pathology analyzed, as well as the disease stage, suggesting posttranscriptional regulation, and several microRNAs were found as potential regulators of MCT1 abundance. MCT1 membrane immunolocalization without cytoplasmic retention was observed in all studied liver pathologies. Overall, the study demonstrates that SLC16A1/MCT1 is involved in liver pathology, especially in ALD.
Agribusiness Facing Its Limits: The Re-Design of Neoliberalization Strategies in the Exporting Agriculture Sector in Chile
Authors: Panez Alexander , Roose Ilka , Faúndez Rodrigo
Published: February 2020

The core neoliberal strategy of Chilean agrarian politics has lasted now for more than 30 years. Despite minor reforms, its fundamental pillars remain in place. While members of the agribusiness sector consider this strategy to be a role-model for food production leading to explosive economic growth, the last decade exposed its socio-ecological limits, such as declining water availability and increased conflicts over land. Taking critical literature on neoliberalization as a theoretical approach, we used law and literature reviews as well as qualitative interviews with actors from the public and private sectors to reveal the details of the strategies in the exporting agriculture sector in Chile. From the understanding of neoliberalization as a multi-layered process, we analyzed the data, focusing on three dimensions of agribusiness in Chile: (a) regulation, (b) spatial fix, and (c) ideological paradigms. In doing so, we uncovered how far the coping strategies chosen by the state and private sector have re-designed and strengthened the process of agriculture neoliberalization in order to push its own socio-ecological limits.
Biological Reactions to Metal Particles and Ions in the Synovial Layer of Mice
Authors: Cheng Xiangyun , Dirmeier Sabine C. , Haßelt Sandra , Baur-Melnyk Andrea , Kretzer Jan Philippe , Bader Rainer , Utzschneider Sandra , Paulus Alexander C.
Published: February 2020

Metal particles and ions released from implants not only have a fundamental effect on the longevity of total joint replacements, but can also be disseminated to remote organs. Periprosthetic tissues harvested during revision surgeries mainly reflect end-stage failure but may not adequately reveal initial biological reactions and systemic side effects. Therefore, primary reactions caused by metal particles and ions were investigated in an established murine model. Left knee joints in three groups, each consisting of ten female BALB/c mice, received injections of metal ions (MI), metal particles (MP) and phosphate-buffered saline (PBS) (control). Seven days after the injection, immunohistochemical analyses of the synovial layer were performed with respect to some biological markers including Tumor necrosis factor -α (TNF-α), Interleukin-6 (IL-6), Interleukin-1β (IL-1β), Cluster of Differentiation 45 (CD45), Cluster of Differentiation 68 (CD68) and Cluster of Differentiation 3(CD3). The MP group showed significantly enhanced proinflammatory cytokine expression (TNF-α, IL-6 and IL-1β) compared with the other groups (p < 0.05). Interestingly, CD3, as a marker for T lymphocytes, did not increase in any of the groups. The MI group showed a significantly increased expression of CD45 compared with the control group (p < 0.05). Therefore, during the primary process, metal particles have stronger pro-inflammatory potential than metal ions, and T lymphocytes did not seem to be activated in our murine model. Systemic reactions caused by metal particles and ions were found by observing the untreated right knees.
Study of Si and Ge Atoms Termination Using H-Dilution in SiGe:H Alloys Deposited by Radio Frequency (13.56 MHz) Plasma Discharge at Low Temperature
Authors: Cosme Ismael , Kosarev Andrey , Zarate-Galvez Saraí , Martinez Hiram E. , Mansurova Svetlana , Kudriavtsev Yuri
Published: February 2020

In this work, we present the study of the atomic composition in amorphous SiXGeY:HZ films deposited by radio frequency (RF—13.56 MHz) plasma discharge at low deposition temperature. A study and control of Si and Ge atoms termination using H-dilution in SiGe:H alloys deposited by RF plasma discharge was conducted and we made a comparison with low-frequency plasma discharge studies. Solid contents of the main elements and contaminants were determined by SIMS technique. It was found that for low dilution rates from RH = 9 to 30, the germanium content in the solid phase strongly depends on the hydrogen dilution and varies from Y = 0.49 to 0.68. On the other hand, with a higher presence of hydrogen in the mixture, the germanium content does not change and remains close to the value of Y = 0.69. The coefficient of Ge preferential incorporation depended on RH and varied from PGe = 0.8 to 4.3. Also, the termination of Si and Ge atoms with hydrogen was studied using FTIR spectroscopy. Preferential termination of Si atoms was observed in the films deposited with low RH < 20, while preferential termination of Ge atoms was found in the films deposited with high RH > 40. In the range of 20 < RH < 40, hydrogen created chemical bonds with both Si and Ge atoms without preference.
Poly(Oxyethylene)-Amidoamine Based Gemini Cationic Surfactants for Oilfield Applications: Effect of Hydrophilicity of Spacer Group
Authors: Hussain S. M. Shakil , Mahboob Ahmad , Kamal Muhammad Shahzad
Published: February 2020

Thermal stability, salt tolerance, and solubility in normal and high salinity brine are the major requirements for any surfactant designed for oilfield applications because the surfactant stays in a non-ambient environment inside the reservoir for a long period of time. Herein, a series of new gemini cationic surfactants (GSs) with varying spacer hydrophilicity were synthesized and elucidated using MALDI-ToF-MS, NMR (1H, 13C), as well as FTIR spectroscopy. GSs found to be soluble in normal as well as high salinity brine and aqueous stability tests revealed that GSs possess the ability to retain their structural integrity at high salinity and high temperature conditions because no suspension formation or precipitation was detected in the oven aged sample of GSs at 90 °C for 30 days. Thermal gravimetric analysis displayed a higher decomposition temperature than the real reservoir temperature and the GS with a secondary amine spacer exhibited high heat stability. The significant reduction in surface tension and critical micelle concentration was observed using 1 M NaCl solution in place of deionized water. The difference in surface tension and critical micelle concentration was insignificant when the 1 M NaCl solution was replaced with seawater. The synthesized surfactants can be utilized for oilfield applications in a challenging high temperature high salinity environment.
Extraction, Identification, Modification, and Antibacterial Activity of Histone from Immature Testis of Atlantic salmon
Authors: Fu Boyu , Lin Hong , Ramesh Pavase Tushar , Mi Nasha , Sui Jianxin
Published: February 2020

In the present study, histone from immature testis of Atlantic salmon was extracted and identified, and its antibacterial activity after enzymolysis was investigated. Histone extracted from Atlantic salmon (Salmo salar) testis using the acid extraction method was successfully identified by LC-MS/MS, and revealed significant inhibitory activity on both the Gram-negative and Gram-positive bacteria. With a low concentration of 10 mg/mL, the observed inhibitory zone diameter (IZD) could significantly reach up to 15.23 mm. After modification of enzymatic hydrolysis by pepsin, histone could be digested to three fragments, while the antibacterial activity increased up to 57.7%. All the results suggested the leftovers from commercial fishing could be utilized for the extraction of antimicrobial peptides.
Alpha-Glucosidase- and Lipase-Inhibitory Phenalenones from a New Species of Pseudolophiostoma Originating from Thailand
Authors: Macabeo Allan Patrick G. , Pilapil Luis Agustin E. , Garcia Katherine Yasmin M. , Quimque Mark Tristan J. , Phukhamsakda Chayanard , Cruz Allaine Jean C. , Hyde Kevin D. , Stadler Marc
Published: February 2020

The alpha-glucosidase- and lipase-inhibitory activities of three phenalenones (1–3) and one phenylpropanoid (4) from the ethyl acetate extracts of a Pseudolophiosptoma sp. are described. They represent the first secondary metabolites reported from the genus Pseudolophiostoma. Scleroderolide (1) and sclerodione (2) exhibited potent α-glucosidase- and porcine-lipase-inhibitory activity during primary screening, with better IC50 values compared to the positive controls, N-deoxynojirimycin and orlistat. In silico techniques were employed to validate the probable biological targets and elucidate the mechanism of actions of phenalenones 1 and 2. Both compounds exhibited strong binding affinities to both alpha-glucosidase and porcine lipase through H-bonding and π–π interactions. Interestingly, favorable in silico ADME (absorption, distribution, metabolism, and excretion) properties such as gastrointestinal absorption were also predicted using software.
Benzoxazole Derivative K313 Induces Cell Cycle Arrest, Apoptosis and Autophagy Blockage and Suppresses mTOR/p70S6K Pathway in Nalm-6 and Daudi Cells
Authors: Zhong Wenying , Tang Xinwen , Liu Yang , Zhou Chunyu , Liu Pan , Li Enhui , Zhong Peilin , Lv Haoxue , Zou Qiang , Wang Maolin
Published: February 2020

Benzoxazole derivative K313 has previously been reported to possess anti-inflammatory effects in lipopolysaccharide-induced RAW264.7 macrophages. To date, there have been no related reports on the anticancer effects of K313. In this study, we found that K313 reduced the viability of human B-cell leukemia (Nalm-6) and lymphoma (Daudi) cells in a dose-dependent manner without affecting healthy peripheral blood mononuclear cells (PBMCs) and induced moderate cell cycle arrest at the G0/G1 phase. Meanwhile, K313 mediated cell apoptosis, which was accompanied by the activation of caspase-9, caspase-3, and poly ADP-ribose polymerase (PARP). Furthermore, cells treated with K313 showed a significant decrease in mitochondrial membrane potential (MMP), which may have been caused by the caspase-8-mediated cleavage of Bid, as detected by Western blot analysis. We also found that K313 led to the downregulation of p-p70S6K protein, which plays an important role in cell survival and cell cycle progression. In addition, treatment of these cells with K313 blocked autophagic flux, as reflected in the accumulation of LC3-II and p62 protein levels in a dose- and time-dependent manner. In conclusion, K313 decreases cell viability without affecting normal healthy PBMCs, induces cell cycle arrest and apoptosis, reduces p-p70S6K protein levels, and mediates strong autophagy inhibition. Therefore, K313 and its derivatives could be developed as potential anticancer drugs or autophagy blockers in the future.
Periodic Mesoporous Organosilica Nanoparticles with BOC Group, towards HIFU Responsive Agents
Authors: Li Hao , Gascó Carolina , Delalande Anthony , Charnay Clarence , Raehm Laurence , Midoux Patrick , Pichon Chantal , Pleixats Roser , Durand Jean-Olivier
Published: February 2020

Periodic Mesoporous Organosilica Nanoparticles (PMONPs) are nanoparticles of high interest for nanomedicine applications. These nanoparticles are not composed of silica (SiO2). They belong to hybrid organic–inorganic systems. We considered using these nanoparticles for CO2 release as a contrast agent for High Intensity Focused Ultrasounds (HIFU). Three molecules (P1–P3) possessing two to four triethoxysilyl groups were synthesized through click chemistry. These molecules possess a tert-butoxycarbonyl (BOC) group whose cleavage in water at 90–100 °C releases CO2. Bis(triethoxysilyl)ethylene E was mixed with the molecules Pn (or not for P3) at a proportion of 90/10 to 75/25, and the polymerization triggered by the sol-gel procedure led to PMONPs. PMONPs were characterized by different techniques, and nanorods of 200–300 nm were obtained. These nanorods were porous at a proportion of 90/10, but non-porous at 75/25. Alternatively, molecules P3 alone led to mesoporous nanoparticles of 100 nm diameter. The BOC group was stable, but it was cleaved at pH 1 in boiling water. Molecules possessing a BOC group were successfully used for the preparation of nanoparticles for CO2 release. The BOC group was stable and we did not observe release of CO2 under HIFU at lysosomal pH of 5.5. The pH needed to be adjusted to 1 in boiling water to cleave the BOC group. Nevertheless, the concept is interesting for HIFU theranostic agents.
Asymmetric Transfer Hydrogenation of Arylketones Catalyzed by Enantiopure Ruthenium(II)/Pybox Complexes Containing Achiral Phosphonite and Phosphinite Ligands
Authors: Claros Miguel , de Julián Eire , Díez Josefina , Lastra Elena , Gamasa M. Pilar
Published: February 2020

A family of complexes of the formula trans-[RuCl2(L)(R-pybox)] (R-pybox = (S,S)-iPr-pybox, (R,R)-Ph-pybox, L = monodentate phosphonite, PPh(OR)2, and phosphinite, L = PPh2(OR), ligands) were screened in the catalytic asymmetric transfer hydrogenation of acetophenone, observing a strong influence of the nature of both the R-pybox substituents and the L ligand in the process. The best results were obtained with complex trans-[RuCl2{PPh2(OEt)}{(R,R)-Ph-pybox}] (2c), which provided high conversion and enantioselectivity (up to 96% enantiomeric excess, e.e.) for the reduction of a variety of aromatic ketones, affording the (S)-benzylalcohols.
The Effect of Thermomechanical Pretreatment on the Structure and Properties of Lignin-Rich Plant Biomass
Authors: Podgorbunskikh Ekaterina M. , Bychkov Aleksey L. , Ryabchikova Elena I. , Lomovsky Oleg I.
Published: February 2020

The cooperative thermomechanical properties of plant-derived polymers have been studied insufficiently, although this feedstock has a very high potential. In the present paper, we analyzed the changes in the structure and physicochemical properties of lignin-rich biomass induced by thermomechanical pretreatment. Low-temperature treatment allows one to retain the original supramolecular structure of the cell walls, while an appreciably high disintegration degree is reached. This increases the reactivity of the material in the subsequent heterogeneous reactions. Mechanical pretreatment at medium temperatures (10 °C), when almost all cell wall polymers except for low-molecular-weight lignin are in the glassy state, enhances the mobility of cell wall polymers and causes sufficient cellulose disordering, while the specific surface area is not significantly increased. High-temperature pretreatment of reed biomass is accompanied by pore formation and lignin release from the cell wall structure, which opens up new prospects for using this biomass as a matrix to produce core–shell-structured sorbents of heavy metals. The energy consumed by mechanochemical equipment for the activation of reed biomass was determined.
MicroRNA-155 Participates in Smoke-Inhalation-Induced Acute Lung Injury through Inhibition of SOCS-1
Authors: Zhang Yue , Xie Yifang , Zhang Leifang , Zhao Hang
Published: February 2020

Smoke inhalation causes acute lung injury (ALI), a severe clinical disease with high mortality. Accumulating evidence indicates that microRNA-155 (miR-155) and suppressor of cytokine signaling 1 (SOCS-1), as mediators of inflammatory response, are involved in the pathogenesis of ALI. In this paper, we explored the proinflammatory mechanism of miR-155 in smoke-inhalation-induced ALI. Our data revealed that smoke inhalation induces miR-155 expression, and miR-155 knockout (KO) significantly ameliorates smoke-inhalation-induced lung injury in mice. Neutrophil infiltration and myeloperoxidase (MPO), macrophage inflammatory protein 2 (MIP-2) and keratinocyte chemoattractant (KC) expressions were decreased in miR-155–/– mice after smoke inhalation as well. Real-time RT-PCR and immunoblotting results showed that SOCS-1 level was remarkably increased in miR-155–/– mice after smoke exposure. Furthermore, the experiments performed in isolated miR-155 KO pulmonary neutrophils demonstrated that the lack of SOCS-1 enhanced inflammatory cytokines (MIP-2 and KC) secretion in response to smoke stimulation. In conclusion, smoke induces increased expression of miR-155, and miR-155 is involved in inflammatory response to smoke-inhalation-induced lung injury by inhibiting the expression of SOCS-1.
The Protective Effect of the Polysaccharide Precursor, D-Isofloridoside, from Laurencia undulata on Alcohol-Induced Hepatotoxicity in HepG2 Cells
Authors: Yang Shengtao , Chen Mei-Fang , Ryu Bomi , Chen Jiali , Xiao Zhenbang , Hong Pengzhi , Sun Shengli , Wang Di , Qian Zhong-Ji , Zhou Chunxia
Published: February 2020

Alcoholic liver disease (ALD) threatens human health, so it is imperative that we find ways to prevent or treat it. In recent years, the study of polysaccharides has shown that they have different kinds of bioactivities. Among them are many biological effects that have been attributed to polysaccharide precursors. D-Isofloridoside (DIF) is one of the polysaccharide precursors from the marine red alga Laurencia undulata. This study evaluated the effect of DIF on alcohol-induced oxidative stress in human hepatoma cells (HepG2). As a result, DIF attenuated alcohol-induced cytotoxicity, reduced the amount of intracellular reactive oxygen species (ROS), and effectively reduced alcohol-induced DNA damage in HepG2 cells. In addition, a western blot showed that, after DIF treatment, the expression levels of glutathione (GSH), superoxide dismutase (SOD), and B-cell lymphoma-2 (bcl-2) increased, while the expression levels of γ-glutamyl transferase (GGT), BCL2-associated X (bax), cleaved caspase-3, and mitogen-activated protein kinase (p38 and c-Jun N-terminal kinase) signal transduction proteins reduced. This showed that DIF may protect cells by reducing the amount of intracellular ROS and inhibiting intracellular oxidative stress and apoptotic processes. Finally, molecular docking demonstrated that DIF can bind to SOD, GGT, B-cell lymphoma-2, and bax proteins. These results indicated that DIF can protect HepG2 cells from alcohol-induced oxidative stress damage, making it an effective potential ingredient in functional foods.
Selected Pharmaceuticals in Different Aquatic Compartments: Part I—Source, Fate and Occurrence
Authors: Pereira André , Silva Liliana , Laranjeiro Célia , Lino Celeste , Pena Angelina
Published: February 2020

Potential risks associated with releases of human pharmaceuticals into the environment have become an increasingly important issue in environmental health. This concern has been driven by the widespread detection of pharmaceuticals in all aquatic compartments. Therefore, 22 pharmaceuticals, 6 metabolites and transformation products, belonging to 7 therapeutic groups, were selected to perform a systematic review on their source, fate and occurrence in different aquatic compartments, important issues to tackle the Water Framework Directive (WFD). The results obtained evidence that concentrations of pharmaceuticals are present, in decreasing order, in wastewater influents (WWIs), wastewater effluents (WWEs) and surface waters, with values up to 14 mg L−1 for ibuprofen in WWIs. The therapeutic groups which presented higher detection frequencies and concentrations were anti-inflammatories, antiepileptics, antibiotics and lipid regulators. These results present a broad and specialized background, enabling a complete overview on the occurrence of pharmaceuticals in the aquatic compartments.
Small Dimension—Big Impact! Nanoparticle-Enhanced Non-Invasive and Intravascular Molecular Imaging of Atherosclerosis In Vivo
Authors: Lenz Tobias , Nicol Philipp , Castellanos Maria Isabel , Engel Leif-Christopher , Lahmann Anna Lena , Alexiou Christoph , Joner Michael
Published: February 2020

Extensive translational research has provided considerable progress regarding the understanding of atherosclerosis pathophysiology over the last decades. In contrast, implementation of molecular in vivo imaging remains highly limited. In that context, nanoparticles represent a useful tool. Their variable shape and composition assure biocompatibility and stability within the environment of intended use, while the possibility of conjugating different ligands as well as contrast dyes enable targeting of moieties of interest on a molecular level and visualization throughout various imaging modalities. These characteristics have been exploited by a number of preclinical research approaches aimed at advancing understanding of vascular atherosclerotic disease, in order to improve identification of high-risk lesions prior to oftentimes fatal thromboembolic events. Furthermore, the combination of these targeted nanoparticles with therapeutic agents offers the potential of site-targeted drug delivery with minimized systemic secondary effects. This review gives an overview of different groups of targeted nanoparticles, designed for in vivo molecular imaging of atherosclerosis as well as an outlook on potential combined diagnostic and therapeutic applications.
Free Radical Mediated Oxidative Degradation of Carotenes and Xanthophylls
Authors: Mordi Raphael C. , Ademosun Olabisi T. , Ajanaku Christiana O. , Olanrewaju Ifedolapo O. , Walton John C.
Published: February 2020

This article reviews the excited-state quenching, pro-vitamin A activity and anticarcinogenicity of carotenes and xanthophylls in relation to their chemical structures. Excited-state quenching improved with the length of the conjugated chain structure. Pro-vitamin A activity was dependent on the presence of at least one beta-ionyl ring structure. The effectiveness of carotenoids as antioxidants depended on their ability to trap peroxyl radicals with production of resonance-stabilized carotenyl radicals. The products identified from oxidations of carotenes and xanthophylls with molecular oxygen and other oxidizing agents are presented. The free radical-mediated mechanisms that have been proposed to account for the different classes of products are reviewed.
Design of Biphenyl-Substituted Diarylpyrimidines with a Cyanomethyl Linker as HIV-1 NNRTIs via a Molecular Hybridization Strategy
Authors: Lei Yuan , Han Sheng , Yang Yang , Pannecouque Christophe , De Clercq Erik , Zhuang Chunlin , Chen Fen-Er
Published: February 2020

The key problems of human immunodeficiency virus (HIV) therapy are the rapid emergence of drug-resistant mutant strains and significant cumulative drug toxicities. Therefore, there is an urgent demand for new anti-HIV agents with low toxicity and broad-spectrum antiviral potency. A series of biphenyl-substituted diarylpyrimidines with a cyanomethyl linker were designed using a molecular hybridization strategy. The cell-based anti-HIV assay showed that most of the compounds exhibited moderate to good activities against wild-type HIV-1 and clinically relevant mutant strains with a more favorable toxicity, and the enzymatic assay showed they had nanomolar activity against reverse transcriptase (RT). Compound 10p exhibited the best activity against wild-type HIV-1 with an EC50 (50% HIV-1 replication inhibitory concentration) value of 0.027 µM, an acceptable CC50 (50% cytotoxic concentration) value of 36.4 µM, and selectivity index of 1361, with moderate activities against the single mutants (EC50: E138K, 0.17 µM; Y181C, 0.87 µM; K103N, 0.9 µM; L100I, 1.21 µM, respectively), and an IC50 value of 0.059 µM against the RT enzyme, which was six-fold higher than nevirapine (NVP). The preliminary structure–activity relationship (SAR) of these new compounds was concluded. The molecular modeling predicted the binding modes of the new compounds with RT, providing molecular insight for further drug design.