bluuebunny/crossref_metadata_2025 huggingface dataset:
Examining Ferromagnetic Materials Subjected to a Static Stress Load Using the Magnetic Method
Authors: Chady Tomasz , Łukaszuk Ryszard
Published: June 2021

This paper discusses the experimental examination of anisotropic steel-made samples subjected to a static stress load. A nondestructive testing (NDT) measurement system with a transducer, which enables observation of local hysteresis loops and detection of samples’ inhomogeneity, is proposed. Local hysteresis loops are measured on two perpendicular axes, including one parallel to the rolling direction of the samples. The results confirm that the selected features of the local hysteresis loops provide important information about the conditions of ferromagnetic materials. Furthermore, it is shown that the selected parameters of the statistical analysis of the achieved measurements are beneficial for evaluating stress and fatigue changes induced in the material.
Building-Information-Modeling Based Approach to Simulate Strategic Location of Shelter in Place and Its Strengthening Method
Authors: Kim Young-Hwi , Choi Jin-Seok , Yuan Tian-Feng , Yoon Young-Soo
Published: June 2021

It is important to consider establishing a shelter in place (SIP) using existing facilities to prepare for unpredictable and no-notice disasters. In this study, we evaluate the building-information-modeling (BIM)-based approach to simulate the strategic location of SIP and its strengthening method. BIM software was used to model a light rail station and analyze the elements of the facility that can affect the evacuation time to reach the SIP. The purpose of this study was to understand the effects of structural standards on the design of SIPs using a direct simulation. The differences between domestic and overseas standards were analyzed. An analysis was carried out to evaluate whether national specifications are satisfactory. As the proposed evacuation method is based on a rational human behavior analysis through a direct simulation, it was going to be a safer and faster route of evacuation in the case of physical terror attack situations for existing infrastructure, Furthermore, the SIP design is considered where reinforcement of the SIP structure is necessary. Three types of reinforcing were considered. Here, the use of high-strength, high-ductility concrete proved to be an effective method to improve the impact resistance of reinforced concrete walls and recommended for strengthening reinforced concrete members.
Effect of a Sulfur Precursor on the Hydrothermal Synthesis of Cu2MnSnS4
Authors: Waluś Edyta , Manecki Maciej , Cios Grzegorz , Tokarski Tomasz
Published: June 2021

Cu2MnSnS4 (CMTS) is acknowledged as an alternative to traditional semiconductors. The structure and microstructure of synthetic CMTS depend on, among other things, the types of sulfur sources used. Traditionally obtained CMTS mostly has a tetragonal structure. In this study, the effect of using thiourea (Tu) or Na2S as a sulfur source on the product structure was compared using hydrothermal synthesis at 190 °C for 7 days (ethylene glycol with water in the presence of poly(vinylpyrollidone) was used as a solvent). When Tu was used, CMTS precipitated in the form of concentric microspheres, 1–1.5 µm in size, consisting of hexagonal (in the cores) and tetragonal (the rims) forms. Most probably, the rapidly formed hexagonal nucleus was later surrounded by a slower-forming rim with a tetragonal structure. In contrast, when Na2S was used as a precursor, microspheres were not formed and a fine crystalline material with a homogeneous tetragonal structure was obtained. This allowed for the choice of micromorphology and product structure during synthesis.
Nanoparticle Tracing during Laser Powder Bed Fusion of Oxide Dispersion Strengthened Steels
Authors: Yang Yangyiwei , Doñate-Buendía Carlos , Oyedeji Timileyin David , Gökce Bilal , Xu Bai-Xiang
Published: June 2021

The control of nanoparticle agglomeration during the fabrication of oxide dispersion strengthened steels is a key factor in maximizing their mechanical and high temperature reinforcement properties. However, the characterization of the nanoparticle evolution during processing represents a challenge due to the lack of experimental methodologies that allow in situ evaluation during laser powder bed fusion (LPBF) of nanoparticle-additivated steel powders. To address this problem, a simulation scheme is proposed to trace the drift and the interactions of the nanoparticles in the melt pool by joint heat-melt-microstructure–coupled phase-field simulation with nanoparticle kinematics. Van der Waals attraction and electrostatic repulsion with screened-Coulomb potential are explicitly employed to model the interactions with assumptions made based on reported experimental evidence. Numerical simulations have been conducted for LPBF of oxide nanoparticle-additivated PM2000 powder considering various factors, including the nanoparticle composition and size distribution. The obtained results provide a statistical and graphical demonstration of the temporal and spatial variations of the traced nanoparticles, showing ∼55% of the nanoparticles within the generated grains, and a smaller fraction of ∼30% in the pores, ∼13% on the surface, and ∼2% on the grain boundaries. To prove the methodology and compare it with experimental observations, the simulations are performed for LPBF of a 0.005 wt % yttrium oxide nanoparticle-additivated PM2000 powder and the final degree of nanoparticle agglomeration and distribution are analyzed with respect to a series of geometric and material parameters.
Improved Process Efficiency in Laser-Based Powder Bed Fusion of Nanoparticle Coated Maraging Tool Steel Powder
Authors: Pannitz Oliver , Großwendt Felix , Lüddecke Arne , Kwade Arno , Röttger Arne , Sehrt Jan Torsten
Published: June 2021

Research and development in the field of metal-based additive manufacturing are advancing steadily every year. In order to increase the efficiency of powder bed fusion of metals using a laser beam system (PBF LB/M), machine manufacturers have implemented extensive optimizations with regard to the laser systems and build volumes. However, the optimization of metallic powder materials using nanoparticle additives enables an additional improvement of the laser–material interaction. In this work, tool steel 1.2709 powder was coated with silicon carbide (SiC), few-layer graphene (FLG), and iron oxide black (IOB) on a nanometer scale. Subsequently, the feedstock material and the modified powder materials were analyzed concerning the reflectance of the laser radiation and processed by PBF-LB/M in a systematic and consistent procedure to evaluate the impact of the nano-additivation on the process efficiency and mechanical properties. As a result, an increased build rate is achieved, exhibiting a relative density of 99.9% for FLG/1.2709 due to a decreased reflectance of this modified powder material. Furthermore, FLG/1.2709 provides hardness values after precipitation hardening with only aging comparable to the original 1.2709 material and is higher than the SiC- and IOB-coated material. Additionally, the IOB coating tends to promote oxide-formation and lack-of-fusion defects.
The Thickness Effect of PSF Nanofibrous Mat on Fracture Toughness of Carbon/Epoxy Laminates
Authors: Saghafi Hamed , Nikbakht Ali , Mohammadi Reza , Zarouchas Dimitrios
Published: June 2021

The geometrical features of nanofibers, such as nanomat thickness and the diameter of nanofibers, have a significant influence on the toughening behavior of composite laminates. In this study, carbon/epoxy laminates were interleaved with polysulfone (PSF) nanofibrous mats and the effect of the PSF nanomat thickness on the fracture toughness was considered for the first time. For this goal, the nanofibers were first produced by the electrospinning method. Then, double cantilever beam (DCB) specimens were manufactured, and mode-I fracture tests were conducted. The results showed that enhancing the mat thickness could increase the fracture toughness considerably (to about 87% with the maximum thickness). The toughening mechanism was also considered by presenting a schematic picture. Micrographs were taken using a scanning electron microscope (SEM).
Spray Flame Synthesis (SFS) of Lithium Lanthanum Zirconate (LLZO) Solid Electrolyte
Authors: Ali Md Yusuf , Orthner Hans , Wiggers Hartmut
Published: June 2021

A spray-flame reaction step followed by a short 1-h sintering step under O2 atmosphere was used to synthesize nanocrystalline cubic Al-doped Li7La3Zr2O12 (LLZO). The as-synthesized nanoparticles from spray-flame synthesis consisted of the crystalline La2Zr2O7 (LZO) pyrochlore phase while Li was present on the nanoparticles’ surface as amorphous carbonate. However, a short annealing step was sufficient to obtain phase pure cubic LLZO. To investigate whether the initial mixing of all cations is mandatory for synthesizing nanoparticulate cubic LLZO, we also synthesized Li free LZO and subsequently added different solid Li precursors before the annealing step. The resulting materials were all tetragonal LLZO (I41/acd) instead of the intended cubic phase, suggesting that an intimate intermixing of the Li precursor during the spray-flame synthesis is mandatory to form a nanoscale product. Based on these results, we propose a model to describe the spray-flame based synthesis process, considering the precipitation of LZO and the subsequent condensation of lithium carbonate on the particles’ surface.
Dynamic Recrystallization and Its Effect on Superior Plasticity of Cold-Rolled Bioabsorbable Zinc-Copper Alloys
Authors: Jarzębska Anna , Maj Łukasz , Bieda Magdalena , Chulist Robert , Wojtas Daniel , Wątroba Maria , Janus Karol , Rogal Łukasz , Sztwiertnia Krzysztof
Published: June 2021

High plasticity of bioabsorbable stents, either cardiac or ureteral, is of great importance in terms of implants’ fabrication and positioning. Zn-Cu constitutes a promising group of materials in terms of feasible deformation since the superplastic effect has been observed in them, yet its origin remains poorly understood. Therefore, it is crucial to inspect the microstructural evolution of processed material to gain an insight into the mechanisms leading to such an extraordinary property. Within the present study, cold-rolled Zn-Cu alloys, i.e., Zn with addition of 1 wt.% and 5 wt.% of Cu, have been extensively investigated using scanning electron microscopy as well as transmission electron microscopy, so as to find out the possible explanation of superior plasticity of the Zn-Cu alloys. It has been stated that the continuous dynamic recrystallization has a tremendous impact on superior plasticity reported for Zn-1Cu alloy processed by rolling to 90% of reduction rate. The effect might be supported by static recrystallization, provoking grain growth and thereby yielding non-homogeneous microstructures. Such heterogeneous microstructure enables better formability since it increases the mean free path for dislocation movement.
Changes in the Strength Properties and Phase Transition of Gypsum Modified with Microspheres, Aerogel and HEMC Polymer
Authors: Ciemnicka Justyna , Prałat Karol , Koper Artur , Makomaski Grzegorz , Majewski Łukasz , Wójcicka Karolina , Buczkowska Katarzyna Ewa
Published: June 2021

The paper presents an assessment of the impact of using additives on the strength of a binding material, i.e., building gypsum, and also the phase transformation that takes place in it. Microspheres, aerogel and polymer (HEMC) additives were added to a building gypsum slurry with a water to gypsum ratio of 0.75. In order to investigate their influence on bending strength, compressive strength, and the effect of high temperatures, differential scanning calorimetry (DSC), as well as tests of the multicomponent binder, were carried out in accordance with the applicable PN-EN 13279-2:2005 standard. The obtained test results allowed to determine that the used additives influenced the strength parameters of the obtained composites. It was shown that the applied additives decreased the compressive and bending strength of the modified gypsum. Despite these properties, the obtained gypsum materials are environmentally friendly because they reuse wastes, such as microspheres. Out of all the applied additives, the use of microspheres in an amount of 10% caused a decrease in the bending strength by only 10%, and an increase in the compressive strength by 4%.
Towards the Bisbenzothienocarbazole Core: A Route of Sulfurated Carbazole Derivatives with Assorted Optoelectronic Properties and Applications
Authors: Bujaldón Roger , Puigdollers Joaquim , Velasco Dolores
Published: June 2021

Ladder-type molecules, which possess an extended aromatic backbone, are particularly sought within the optoelectronic field. In view of the potential of the 14H-bis[1]benzothieno[3,2-b:2’,3’-h]carbazole core as a p-type semiconductor, herein we studied a set of two derivatives featuring a different alkylation patterning. The followed synthetic route, involving various sulfurated carbazole-based molecules, also resulted in a source of fluorophores with different emitting behaviors. Surprisingly, the sulfoxide-containing fluorophores substantially increased their blue fluorescence with respect to the nearly non-emitting sulfur counterparts. On this basis, we could shed light on the relationship between their chemical structure and their emission as an approach for future applications. Considering the performance in organic thin-film transistors, both bisbenzothienocarbazole derivatives displayed p-type characteristics, with hole mobility values up to 1.1 × 10−3 cm2 V−1 s−1 and considerable air stability. Moreover, the role of the structural design has been correlated with the device performance by means of X-ray analysis and the elucidation of the corresponding single crystal structures.
Recovery of Industrial Wastes as Fillers in the Epoxy Thermosets for Building Application
Authors: Hodul Jakub , Mészárosová Lenka , Drochytka Rostislav
Published: June 2021

Epoxy resins are currently used in many areas of construction, such as resistant coatings, anchors, fibre-reinforced polymer (FRP) composites, grouts, etc. This paper deals mainly with epoxy composites that can be applied during the rehabilitation of concrete constructions. The influence of a filler type on epoxy thermoset composites was monitored, whilst three different types of epoxy resin were used in order to achieve a better representation and confirmation of the results. During the testing of fillers, these were mainly secondary raw materials, including pre-treated hazardous waste (neutralisation sludge), representing various shapes and sizes of particle, while their amount in the epoxy matrix was chosen with regard to optimal viscosity and workability. Physical and mechanical parameters, like compressive and flexural strengths, cohesion with the concrete and thermal expansion of the epoxy composites containing various fillers were determined. The microstructure of epoxy composites with a different filler type and chemical resistance against chemical aggressive media were all monitored. The microstructure of epoxy composites was monitored using scanning electron microscopy (SEM) supported by energy-dispersive X-ray spectroscopy (EDX). Computed tomography (CT) was also used for the evaluation of the cohesion of the epoxy composites with concrete and dispersion of the filler in the epoxy matrix.
On the Superconductivity Suppression in Eu1−xPrxBa2Cu3O7−δ
Authors: Pęczkowski Paweł , Zachariasz Piotr , Jastrzębski Cezariusz , Piętosa Jarosław , Drzymała Elżbieta , Gondek Łukasz
Published: June 2021

This article reports on the non-trivial suppression of superconductivity in the Eu1−xPrxBCO cuprates. As non-magnetic Eu3+ ions are replaced by Pr3+ carrying a magnetic moment, spin-related superconductivity loss can be expected. The research shows that the superconductivity disappearance for x > 0.4 results from depletion of the carriers and their localization. The above conclusion was drawn by low-temperature X-ray diffraction analysis showing increased characteristic phonon frequencies with Pr content. This mechanism should promote electron–phonon coupling, at least for acoustic phonons. However, the inverse phenomenon was detected. Namely, there is a gradual deterioration of the optical phonons responsible for vibration of the Cu–O bonds with Pr increasing, as evidenced by Raman spectroscopy. Furthermore, the results of X-ray absorption spectroscopy precisely showed the location of the carriers for Pr-rich specimens. Finally, a schematic diagram for Eu1−xPrxBCO is proposed to consolidate the presented research.
Simulation of Slip-Oxidation Process by Mesh Adaptivity in a Cohesive Zone Framework
Authors: Sedlak Mosesson Michal , Alfredsson Bo , Efsing Pål
Published: June 2021

Adaptive oxide thickness was developed in a cohesive element based multi-physics model including a slip-oxidation and diffusion model. The model simulates the intergranular stress corrosion cracking (IGSCC) in boiling water reactors (BWR). The oxide thickness was derived from the slip-oxidation and updated in every structural iteration to fully couple the fracture properties of the cohesive element. The cyclic physics of the slip oxidation model was replicated. In the model, the thickness of the oxide was taken into consideration as the physical length of the cohesive element. The cyclic process was modelled with oxide film growth, oxide rupture, and re-passivation. The model results agreed with experiments in the literature for changes in stress intensity factor, yield stress representing cold work, and environmental factors such as conductivity and corrosion potential.
First Molecular Superconductor with the Tris(Oxalato)Aluminate Anion, β″-(BEDT-TTF)4(H3O)Al(C2O4)3·C6H5Br, and Isostructural Tris(Oxalato)Cobaltate and Tris(Oxalato)Ruthenate Radical Cation Salts
Authors: Blundell Toby James , Brannan Michael , Mburu-Newman Joey , Akutsu Hiroki , Nakazawa Yasuhiro , Imajo Shusaku , Martin Lee
Published: June 2021

Peter Day’s research group reported the first molecular superconductor containing paramagnetic metal ions in 1995, β″-(BEDT-TTF)4(H3O)Fe(C2O4)3·C6H5CN. Subsequent research has produced a multitude of BEDT-TTF-tris(oxalato)metallate salts with a variety of structures and properties, including 32 superconductors to date. We present here the synthesis, crystal structure, and conducting properties of the newest additions to the Day series including the first superconductor incorporating the diamagnetic tris(oxalato)aluminate anion, β″-(BEDT-TTF)4(H3O)Al(C2O4)3·C6H5Br, which has a superconducting Tc of ~2.5 K. β″-(BEDT-TTF)4(H3O)Co(C2O4)3·C6H5Br represents the first example of a β″ phase for the tris(oxalato)cobaltate anion, but this salt does not show superconductivity.
A Constrained Markovian Diffusion Model for Controlling the Pollution Accumulation
Authors: Escobedo-Trujillo Beatris Adriana , López-Barrientos José Daniel , Garrido-Meléndez Javier
Published: June 2021

This work presents a study of a finite-time horizon stochastic control problem with restrictions on both the reward and the cost functions. To this end, it uses standard dynamic programming techniques, and an extension of the classic Lagrange multipliers approach. The coefficients considered here are supposed to be unbounded, and the obtained strategies are of non-stationary closed-loop type. The driving thread of the paper is a sequence of examples on a pollution accumulation model, which is used for the purpose of showing three algorithms for the purpose of replicating the results. There, the reader can find a result on the interchangeability of limits in a Dirichlet problem.
A Novel Methodology for Adaptive Coordination of Multiple Controllers in Electrical Grids
Authors: Tapia-Olvera Ruben , Beltran-Carbajal Francisco , Valderrabano-Gonzalez Antonio , Aguilar-Mejia Omar
Published: June 2021

This proposal is aimed to overcome the problem that arises when diverse regulation devices and controlling strategies are involved in electric power systems regulation design. When new devices are included in electric power system after the topology and regulation goals were defined, a new design stage is generally needed to obtain the desired outputs. Moreover, if the initial design is based on a linearized model around an equilibrium point, the new conditions might degrade the whole performance of the system. Our proposal demonstrates that the power system performance can be guaranteed with one design stage when an adequate adaptive scheme is updating some critic controllers’ gains. For large-scale power systems, this feature is illustrated with the use of time domain simulations, showing the dynamic behavior of the significant variables. The transient response is enhanced in terms of maximum overshoot and settling time. This is demonstrated using the deviation between the behavior of some important variables with StatCom, but without or with PSS. A B-Spline neural networks algorithm is used to define the best controllers’ gains to efficiently attenuate low frequency oscillations when a short circuit event is presented. This strategy avoids the parameters and power system model dependency; only a dataset of typical variable measurements is required to achieve the expected behavior. The inclusion of PSS and StatCom with positive interaction, enhances the dynamic performance of the system while illustrating the ability of the strategy in adding different controllers in only one design stage.
MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents
Authors: Pistelli Luigi , Sansone Clementina , Smerilli Arianna , Festa Marco , Noonan Douglas M. , Albini Adriana , Brunet Christophe
Published: June 2021

Photochemoprevention can be a valuable approach to counteract the damaging effects of environmental stressors (e.g., UV radiations) on the skin. Pigments are bioactive molecules, greatly attractive for biotechnological purposes, and with promising applications for human health. In this context, marine microalgae are a valuable alternative and eco-sustainable source of pigments that still need to be taken advantage of. In this study, a comparative in vitro photochemopreventive effects of twenty marine pigments on carcinogenic melanoma model cell B16F0 from UV-induced injury was setup. Pigment modulation of the intracellular reactive oxygen species (ROS) concentration and extracellular release of nitric oxide (NO) was investigated. At the cell signaling level, interleukin 1-β (IL-1β) and matrix metallopeptidase 9 protein (MMP-9) protein expression was examined. These processes are known to be involved in the signaling pathway, from UV stress to cancer induction. Diatoxanthin resulted the best performing pigment in lowering MMP-9 levels and was able to strongly lower IL-1β. This study highlights the pronounced bioactivity of the exclusively aquatic carotenoid diatoxanthin, among the others. It is suggested increasing research efforts on this molecule, emphasizing that a deeper integration of plant ecophysiological studies into a biotechnological context could improve the exploration and exploitation of bioactive natural products.
Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity
Authors: Conde Tiago A. , Neves Bruna F. , Couto Daniela , Melo Tânia , Neves Bruno , Costa Margarida , Silva Joana , Domingues Pedro , Domingues M. Rosário
Published: June 2021

The demand for sustainable and environmentally friendly food sources and food ingredients is increasing, and microalgae are promoted as a sustainable source of essential and bioactive lipids, with high levels of omega-3 fatty acids (ω-3 FA), comparable to those of fish. However, most FA screening studies on algae are scattered or use different methodologies, preventing a true comparison of its content between microalgae. In this work, we used gas-chromatography mass-spectrometry (GC-MS) to characterize the FA profile of seven different commercial microalgae with biotechnological applications (Chlorella vulgaris, Chlorococcum amblystomatis, Scenedesmus obliquus, Tetraselmis chui, Phaeodactylum tricornutum, Spirulina sp., and Nannochloropsis oceanica). Screening for antioxidant activity was also performed to understand the relationship between FA profile and bioactivity. Microalgae exhibited specific FA profiles with a different composition, namely in the ω-3 FA profile, but with species of the same phylum showing similar tendencies. The different lipid extracts showed similar antioxidant activities, but with a low activity of the extracts of Nannochloropsis oceanica. Overall, this study provides a direct comparison of FA profiles between microalgae species, supporting the role of these species as alternative, sustainable, and healthy sources of essential lipids.
Bioactivity of Chitosan-Based Particles Loaded with Plant-Derived Extracts for Biomedical Applications: Emphasis on Antimicrobial Fiber-Based Systems
Authors: Antunes Joana C. , Domingues Joana M. , Miranda Catarina S. , Silva A. Francisca G. , Homem Natália C. , Amorim M. Teresa P. , Felgueiras Helena P.
Published: June 2021

Marine-derived chitosan (CS) is a cationic polysaccharide widely studied for its bioactivity, which is mostly attached to its primary amine groups. CS is able to neutralize reactive oxygen species (ROS) from the microenvironments in which it is integrated, consequently reducing cell-induced oxidative stress. It also acts as a bacterial peripheral layer hindering nutrient intake and interacting with negatively charged outer cellular components, which lead to an increase in the cell permeability or to its lysis. Its biocompatibility, biodegradability, ease of processability (particularly in mild conditions), and chemical versatility has fueled CS study as a valuable matrix component of bioactive small-scaled organic drug-delivery systems, with current research also showcasing CS’s potential within tridimensional sponges, hydrogels and sutures, blended films, nanofiber sheets and fabric coatings. On the other hand, renewable plant-derived extracts are here emphasized, given their potential as eco-friendly radical scavengers, microbicidal agents, or alternatives to antibiotics, considering that most of the latter have induced bacterial resistance because of excessive and/or inappropriate use. Loading them into small-scaled particles potentiates a strong and sustained bioactivity, and a controlled release, using lower doses of bioactive compounds. A pH-triggered release, dependent on CS’s protonation/deprotonation of its amine groups, has been the most explored stimulus for that control. However, the use of CS derivatives, crosslinking agents, and/or additional stabilization processes is enabling slower release rates, following extract diffusion from the particle matrix, which can find major applicability in fiber-based systems within ROS-enriched microenvironments and/or spiked with microbes. Research on this is still in its infancy. Yet, the few published studies have already revealed that the composition, along with an adequate drug release rate, has an important role in controlling an existing infection, forming new tissue, and successfully closing a wound. A bioactive finishing of textiles has also been promoting high particle infiltration, superior washing durability, and biological response.
Antiviral Nanomaterials for Designing Mixed Matrix Membranes
Authors: Alayande Abayomi Babatunde , Kang Yesol , Jang Jaewon , Jee Hobin , Lee Yong-Gu , Kim In S. , Yang Euntae
Published: June 2021

Membranes are helpful tools to prevent airborne and waterborne pathogenic microorganisms, including viruses and bacteria. A membrane filter can physically separate pathogens from air or water. Moreover, incorporating antiviral and antibacterial nanoparticles into the matrix of membrane filters can render composite structures capable of killing pathogenic viruses and bacteria. Such membranes incorporated with antiviral and antibacterial nanoparticles have a great potential for being applied in various application scenarios. Therefore, in this perspective article, we attempt to explore the fundamental mechanisms and recent progress of designing antiviral membrane filters, challenges to be addressed, and outlook.
The Det.Belt Server: A Tool to Visualize and Estimate Amphipathic Solvent Belts around Membrane Proteins
Authors: Zampieri Veronica , Hilpert Cécile , Garnier Mélanie , Gestin Yannick , Delolme Sébastien , Martin Juliette , Falson Pierre , Launay Guillaume , Chaptal Vincent
Published: June 2021

Detergents wrap around membrane proteins to form a belt covering the hydrophobic part of the protein serving for membrane insertion and interaction with lipids. The number of detergent monomers forming this belt is usually unknown to investigators, unless dedicated detergent quantification is undertaken, which for many projects is difficult to setup. Yet, having an approximate knowledge of the amount of detergent forming the belt is extremely useful, to better grasp the protein of interest in interaction with its direct environment rather than picturing the membrane protein “naked”. We created the Det.Belt server to dress up membrane proteins and represent in 3D the bulk made by detergent molecules wrapping in a belt. Many detergents are included in a database, allowing investigators to screen in silico the effect of different detergents around their membrane protein. The input number of detergents is changeable with fast recomputation of the belt for interactive usage. Metrics representing the belt are readily available together with scripts to render quality 3D images for publication. The Det.Belt server is a tool for biochemists to better grasp their sample.
Microstructure and Texture Development in Thermomechanically Processed Leaded Brass
Authors: Al-Fadhalah Khaled J. , Aleem Rafeeq Muhammad , Thomas Nicky
Published: June 2021

Leaded brass alloys with high zinc content are classified as dual-phase (α-β′) brass alloys. For these alloys, α-β′ phase transformation induced by thermomechanical processing (TMP) is expected to play a significant role not only in the formation of α and β′ phases and uniformity of lead particles but also in the development of Σ3 boundaries and their variants in the α phase. To assess such impact, the current study has employed two TMP schemes, with each scheme consisting of four cycles of compression and annealing. Samples of the first scheme (TMP-1) were annealed at 670 °C to promote phase transformation from α to β′, as compared to the second scheme (TMP-2) samples annealed at 525 °C. Microstructure examination indicated that TMP-1 scheme fostered a remarkable growth in the area fraction of β′ phase, accompanied by grain coarsening of α and β′ phases. This resulted in the isolation of α grains and less interaction of Σ3 boundaries at trip junctions, and therefore, a reduction in the fraction of Σ3 boundaries occurred in the α phase. On the other hand, the TMP-2 scheme successfully enhanced cluster formation of α grains, enabling further generation of Σ3 boundaries via strain-induced boundary migration mechanism and ultimately leading to breakup of the random boundary network. In addition, the two schemes resulted in limited coarsening of lead particles. Most lead particles were located at the α-βʹ interfaces, which suppressed grain boundary migration via the particle-dragging effect. For both TMP schemes, the initial texture of tension fibers was largely preserved after four cycles for both α and βʹ phases. Nevertheless, the TMP-2 scheme produced texture randomization of the α phase due to increasing the fraction of Σ3 boundaries.
Forecasting Model of Silicon Content in Molten Iron Using Wavelet Decomposition and Artificial Neural Networks
Authors: Diniz Ana P. Miranda , Côco Klaus Fabian , Gomes Flávio S. Vitorino , Salles José L. Félix
Published: June 2021

Silicon content forecasting models have been requested by the operational team to anticipate necessary actions during the blast furnace operation when producing molten iron, to control the quality of the product and reduce costs. This paper proposed a new algorithm to perform the silicon content time series up to 8 h ahead, immediately after the molten iron chemical analysis is delivered by the laboratory. Due to the delay of the laboratory when delivering the silicon content measurement, the proposed algorithm considers a minimum useful forecasting horizon of 3 h ahead. In a first step, it decomposes the silicon content time series into different subseries using the Maximal Overlap Discrete Wavelet Packet Transform (MODWPT). Next, all subseries forecasts were determined through Nonlinear Autoregressive (NAR) networks, and finally, these forecasts were summed to furnish the long-term forecast of silicon content. Using data from a real industry, we showed that the prediction error was within an acceptable range according to the blast furnace technical team.
Low-Cycle Fatigue Behavior of Hot-Bent Basal Textured AZ31B Wrought Magnesium Alloy
Authors: Nischler Anton , Denk Josef , Saage Holger , Klaus Hubert , Huber Otto
Published: June 2021

In the recent past, several researchers have successfully modeled the complex fatigue behavior of planar twin-roll cast AZ31B alloy sheets. Complex components are usually hot-bent, whereby the microstructure in the hot-bent areas changes significantly. However, studies on the fatigue behavior of hot-bent magnesium alloys are currently lacking. Therefore, a novel, uniaxial hot-bent specimen was developed and optimized with finite element method simulations. Microstructural analyses with the electron backscatter diffraction method reveal that the hot-bending process changes the texture and increases the Schmid factor for basal slip in rolling and transverse direction of the sheet. In the subsequent quasi-static tension and compression tests, anisotropic and asymmetric yield stresses, lower Young’s moduli compared with the as-received material and macroscopic bands of twinned grains are obtained. Finally, the study proves that the recently proposed concept of highly strained volume can accurately estimate the lifetime, even by combining the as-received and hot-bent material in one fatigue model.
Analysis of Host Jejunum Transcriptome and Associated Microbial Community Structure Variation in Young Calves with Feed-Induced Acidosis
Authors: Gaowa Naren , Li Wenli , Gelsinger Sonia , Murphy Brianna , Li Shengli
Published: June 2021

Diet-induced acidosis imposes a health risk to young calves. In this study, we aimed to investigate the host jejunum transcriptome changes, along with its microbial community variations, using our established model of feed-induced ruminal acidosis in young calves. Eight bull calves were randomly assigned to two diet treatments beginning at birth (a starch-rich diet, Aci; a control diet, Con). Whole-transcriptome RNA sequencing was performed on the jejunum tissues collected at 17 weeks of age. Ribosomal RNA reads were used for studying microbial community structure variations in the jejunum. A total of 853 differentially expressed genes were identified (402 upregulated and 451 downregulated) between the two groups. The cell cycle and the digestion and absorption of protein in jejunal tissue were affected by acidosis. Compared to the control, genera of Campylobacter, Burkholderia, Acidaminococcus, Corynebacterium, and Olsenella significantly increased in abundance in the Aci group, while Lachnoclostridium and Ruminococcus were significantly lower in the Aci group. Expression changes in the AXL gene were associated with the abundance variations of a high number of genera in jejunum. Our study provided a snapshot of the transcriptome changes in the jejunum and its associated meta-transcriptome changes in microbial communities in young calves with feed-induced acidosis.
Nanoscale Vacuum Diode Based on Thermionic Emission for High Temperature Operation
Authors: Shen Zhihua , Li Qiaoning , Wang Xiao , Tian Jinshou , Wu Shengli
Published: June 2021

Vacuum diodes, based on field emission mechanisms, demonstrate a superior performance in high-temperature operations compared to solid-state devices. However, when considering low operating voltage and continuous miniaturization, the cathode is usually made into a tip structure and the gap between cathode and anode is reduced to a nanoscale. This greatly increases the difficulty of preparation and makes it difficult to ensure fabrication consistency. Here, a metal-insulator-semiconductor (MIS) structural nanoscale vacuum diode, based on thermionic emission, was numerically studied. The results indicate that this device can operate at a stable level in a wide range of temperatures, at around 600 degrees Kelvin above 260 K at 0.2 V voltage bias. Moreover, unlike the conventional vacuum diodes working in field emission regime where the emission current is extremely sensitive to the gap-width between the cathode and the anode, the emission current of the proposed diode shows a weak correlation to the gap-width. These features make this diode a promising alternative to vacuum electronics for large-scale production and harsh environmental applications.
Plasmodium matutinum Causing Avian Malaria in Lovebirds (Agapornis roseicollis) Hosted in an Italian Zoo
Authors: Cocumelli Cristiano , Iurescia Manuela , Diaconu Elena Lavinia , Galietta Valentina , Raso Caterina , Buccella Carmela , Stravino Fiorentino , Grande Francesco , Fiorucci Letizia , De Liberato Claudio , Caprioli Andrea , Battisti Antonio
Published: June 2021

Avian malaria is a worldwide distributed, vector-born disease of birds caused by parasites of the order Haemosporida. There is a lack of knowledge about the presence and pathogenetic role of Haemosporida in Psittacidae. Here we report a case of avian malaria infection in lovebirds (Agapornis roseicollis), with the genetic characterization of the Plasmodium species involved. The birds were hosted in a zoo located in Italy, where avian malaria cases in African penguins (Spheniscus demersus) were already reported. Animals (n = 11) were submitted for necropsy after sudden death and were subjected to further analyses including histopathology, bacteriology, and PCR for the research of haemosporidians. Clinical history, gross lesions and histopathological observation of schizonts, together with positive PCR results for Plasmodium spp., demonstrated that avian malaria was the cause of death for one animal and the possible cause of death for the other nine. The sequences obtained were compared using BLAST and analyzed for similarity to sequences available at the MalAvi database. Genetic analyses demonstrated a 100% nucleotide identity to Plasmodium matutinum LINN1 for all the obtained sequences. To our knowledge, this is the first report describing avian malaria in lovebirds.
Correction: Szopinska-Tokov et al. Investigating the Gut Microbiota Composition of Individuals with Attention-Deficit/Hyperactivity Disorder and Association with Symptoms. Microorganisms 2020, 8, 406
Authors: Szopinska-Tokov Joanna , Dam Sarita , Naaijen Jilly , Konstanti Prokopis , Rommelse Nanda , Belzer Clara , Buitelaar Jan , Franke Barbara , Bloemendaal Mirjam , Aarts Esther , Arias Vasquez Alejandro
Published: June 2021

The authors wish to make the following correction to this paper [...]
Procaryotic Diversity and Hydrogenotrophic Methanogenesis in an Alkaline Spring (La Crouen, New Caledonia)
Authors: Quéméneur Marianne , Mei Nan , Monnin Christophe , Postec Anne , Wils Laura , Bartoli Manon , Guasco Sophie , Pelletier Bernard , Erauso Gael
Published: June 2021

(1) Background: The geothermal spring of La Crouen (New Caledonia) discharges warm (42 °C) alkaline water (pH~9) enriched in dissolved nitrogen with traces of methane, but its microbial diversity has not yet been studied. (2) Methods: Cultivation-dependent and -independent methods (e.g., Illumina sequencing and quantitative PCR based on 16S rRNA gene) were used to describe the prokaryotic diversity of this spring. (3) Results: Prokaryotes were mainly represented by Proteobacteria (57% on average), followed by Cyanobacteria, Chlorofexi, and Candidatus Gracilibacteria (GN02/BD1-5) (each > 5%). Both potential aerobes and anaerobes, as well as mesophilic and thermophilic microorganisms, were identified. Some of them had previously been detected in continental hyperalkaline springs found in serpentinizing environments (The Cedars, Samail, Voltri, and Zambales ophiolites). Gammaproteobacteria, Ca. Gracilibacteria and Thermotogae were significantly more abundant in spring water than in sediments. Potential chemolithotrophs mainly included beta- and gammaproteobacterial genera of sulfate-reducers (Ca. Desulfobacillus), methylotrophs (Methyloversatilis), sulfur-oxidizers (Thiofaba, Thiovirga), or hydrogen-oxidizers (Hydrogenophaga). Methanogens (Methanobacteriales and Methanosarcinales) were the dominant Archaea, as found in serpentinization-driven and deep subsurface ecosystems. A novel alkaliphilic hydrogenotrophic methanogen (strain CAN) belonging to the genus Methanobacterium was isolated, suggesting that hydrogenotrophic methanogenesis occurs at La Crouen.
The Two-Component System 09 of Streptococcus pneumoniae Is Important for Metabolic Fitness and Resistance during Dissemination in the Host
Authors: Hirschmann Stephanie , Gómez-Mejia Alejandro , Kohler Thomas P. , Voß Franziska , Rohde Manfred , Brendel Max , Hammerschmidt Sven
Published: June 2021

The two-component regulatory system 09 of Streptococcus pneumoniae has been shown to modulate resistance against oxidative stress as well as capsule expression. These data and the implication of TCS09 in cell wall integrity have been shown for serotype 2 strain D39. Other data have suggested strain-specific regulatory effects of TCS09. Contradictory data are known on the impact of TCS09 on virulence, but all have been explored using only the rr09-mutant. In this study, we have therefore deleted one or both components of the TCS09 (SP_0661 and SP_0662) in serotype 4 S. pneumoniae TIGR4. In vitro growth assays in chemically defined medium (CDM) using sucrose or lactose as a carbon source indicated a delayed growth of nonencapsulated tcs09-mutants, while encapsulated wild-type TIGR4 and tcs09-mutants have reduced growth in CDM with glucose. Using a set of antigen-specific antibodies, immunoblot analysis showed that only the pilus 1 backbone protein RrgB is significantly reduced in TIGR4ΔcpsΔhk09. Electron microscopy, adherence and phagocytosis assays showed no impact of TCS09 on the TIGR4 cell morphology and interaction with host cells. In contrast, in vivo infections and in particular competitive co-infection experiments demonstrated that TCS09 enhances robustness during dissemination in the host by maintaining bacterial fitness.
Effect of Fungicide Application on Lowbush Blueberries Soil Microbiome
Authors: Lloyd Austin W. , Percival David , Yurgel Svetlana N.
Published: June 2021

Lowbush blueberries (Vaccinium sp.) are perennial crops produced throughout eastern Canada and Maine through management of wild populations. Given the constraints of this cropping system, the application of fungicides is critical to reducing disease pressure and ensuring consistent yields. However, as plant health is intertwined with soil health, it is important to consider the impact of fungicides on microbial communities. To understand the effects of fungicides in this context, bacterial and fungal microbial communities from fungicide-treated plots, as well as untreated control plots (UTG) were analyzed using amplicon sequencing. The fungicides, considered collectively as a combined treatment group (CTG), lead to a loss in fungal richness. One family, Clavariaceae, had an increased abundance under prothioconazole relative to UTG. This finding may be significant as taxa in Clavariaceae have been thought to potentially form ericoid mycorrhizae with Vaccinium. Five functional pathways and 74 enzymes differed significantly in relative abundance between CTG and UTG including enzymes associated with soil nutrient cycles. Most notably, enzymes corresponding to the breakdown of halogen-organic compounds had an increased abundance in CTG, suggesting bacterial fungicide degradation. Some enzymes associated with soil nutrient cycles differed significantly, possibly implying changes to nutrient pathways due to fungicide treatment.
Reactive Transport Simulation of Low-pH Cement Interacting with Opalinus Clay Using a Dual Porosity Electrostatic Model
Authors: Jenni Andreas , Mäder Urs
Published: June 2021

Strong chemical gradients between clay and concrete porewater lead to diffusive transport across the interface and subsequent mineral reactions in both materials. These reactions may influence clay properties such as swelling behaviour, permeability or radionuclide retention, which are relevant for the safety of a radioactive waste repository. Different cement types lead to different interactions with Opalinus Clay (OPA), which must be understood to choose the most suitable material. The consideration of anion-depleted porosity due to electrostatic repulsion in clay modelling substantially influences overall diffusive transport and pore clogging at interfaces. The identical dual porosity model approach previously used to predict interaction between Portland cement and OPA is now applied to low-alkali cement—OPA interaction. The predictions are compared with corresponding samples from the cement-clay interaction (CI) experiment in the Mont Terri underground rock laboratory (Switzerland). Predicted decalcification of the cement at the interface (depletion of C–S–H and absence of ettringite within 1 mm from the interface), the Mg enrichment in clay and cement close to the interface (neoformation of up to 17 vol% Mg hydroxides in concrete, and up to 6 vol% in OPA within 0.6 mm at the interface), and the slightly increased S content in the cement 3–4 mm away from the interface qualitatively match the sample characterisation. Simulations of Portland cement—OPA interaction indicate a weaker chemical disturbance over a larger distance compared with low-pH cement—OPA. In the latter case, local changes in porosity are stronger and lead to predicted pore clogging.
Dusty Plasma in Inhomogeneous Magnetic Fields in a Stratified Glow Discharge
Authors: Dzlieva Elena S. , D’yachkov Lev G. , Novikov Leontiy A. , Pavlov Sergey I. , Karasev Viktor Y.
Published: June 2021

We study the dynamics of dust particles in a stratified glow discharge in inhomogeneous magnetic fields. Dust structures are formed in standing striations, in which traps for dust particles arise. When a magnetic field is applied, these structures begin to rotate. The observations were carried out in striations near the end of the solenoid, where the region of an inhomogeneous magnetic field begins. With an increase in the magnetic field, the dusty structure can be deformed. The rotation of a dusty structure in an inhomogeneous magnetic field has been studied in detail; it has its own peculiarities in comparison with rotation in a uniform field. We have considered the mechanisms of such rotation and estimated its velocity.