bluuebunny/crossref_metadata_2025 huggingface dataset:
Rosmarinus officinalis and Methylphenidate Exposure Improves Cognition and Depression and Regulates Anxiety-Like Behavior in AlCl3-Induced Mouse Model of Alzheimer’s Disease
Authors: Malik Nishat , Amber Sanila , Zahid Saadia
Published: August 2022

Alzheimer’s disease (AD) is a neurological illness that causes severe cognitive impairment. AD patients also experience at least one of the neuropsychiatric symptoms including apathy, depression, and anxiety during the course of their life. Acetylcholine esterase inhibitors are the available treatment options to alleviate cognitive deficits, whereas methylphenidate (MPH), a psychostimulant, is considered for the treatment of apathy in AD patients. Rosmarinus officinalis, a perennial herb, has been potentially known to have antioxidant and anti-inflammatory properties. The present study investigated the potential effects of MPH and R. officinalis in comparison with the standard drug, Donepezil, on cognition, anxiety, and depression in the AlCl3-induced mouse model of AD. The animals were divided into eight groups (n = 8, each). The results revealed that the MPH- and R. officinalis-treated groups significantly improved memory impairment, whereas R. officinalis substantially reduced depression and anxiety as compared with other treatment groups. MPH treatment induced an antidepressant effect and increased anxiety-like behavior. Moreover, the AlCl3 exposure led to the formation of amyloid beta (Aβ) plaques in mice hippocampus; however, none of the tested drugs caused a significant reduction in amyloid burden at the selected doses. The present study suggested the potential of R. officinalis to improve memory as well as neuropsychiatric symptoms in AD. Although R. officinalis improved cognitive abilities, it did not reduce the amyloid plaque burden, which indicates that the memory-enhancing effects of R. officinalis are due to some alternate mechanism that needs to be explored further.
Design, synthesis, and biological characterization of a potent STAT3 degrader for the treatment of gastric cancer
Authors: Li Haobin , Wang Lingling , Cao Fei , Yu Dehua , Yang Jing , Yu Xuefei , Dong Jinyun , Qin Jiang-Jiang , Guan Xiaoqing
Published: August 2022

Gastric cancer is a common malignant tumor that threatens human health, and its occurrence and development mechanism is a complex process involving multiple genes and multiple signals. Signal transducer and activator of transcription 3 (STAT3) has been elucidated as a promising target for developing anticancer drugs in gastric cancer. However, there is no FDA-approved STAT3 inhibitor yet. Herein, we report the design and synthesis of a class of STAT3 degraders based on proteolysis-targeting chimeras (PROTACs). We first synthesized an analog of the STAT3 inhibitor S3I-201 as a ligand, using the cereblon (CRBN)/cullin 4A E3 ligase ligand pomalidomide to synthesize a series of PROTACs. Among them, the SDL-1 achieves the degradation of STAT3 protein in vitro, and exhibits good anti-gastric cancer cell proliferation activity, inhibits invasion and metastasis of MKN1 cell, and induces MKN1 cell apoptosis and arrests cell cycle at the same time. Our study shows that SDL-1 is a potent STAT3 degrader and may serve as a potential anti-gastric cancer drug, providing ideas for further development of drugs for clinical use.
Contribution of muscle satellite cells to sarcopenia
Authors: Huo Fengjiao , Liu Qing , Liu Hailiang
Published: August 2022

Sarcopenia, a disorder characterized by age-related muscle loss and reduced muscle strength, is associated with decreased individual independence and quality of life, as well as a high risk of death. Skeletal muscle houses a normally mitotically quiescent population of adult stem cells called muscle satellite cells (MuSCs) that are responsible for muscle maintenance, growth, repair, and regeneration throughout the life cycle. Patients with sarcopenia are often exhibit dysregulation of MuSCs homeostasis. In this review, we focus on the etiology, assessment, and treatment of sarcopenia. We also discuss phenotypic and regulatory mechanisms of MuSC quiescence, activation, and aging states, as well as the controversy between MuSC depletion and sarcopenia. Finally, we give a multi-dimensional treatment strategy for sarcopenia based on improving MuSC function.
Modulation of neuromuscular excitability in response to acute noxious heat exposure has no additional effects on central and peripheral fatigability
Authors: Eimantas Nerijus , Ivanove Soneta , Baranauskiene Neringa , Solianik Rima , Brazaitis Marius
Published: August 2022

Background: Whole-body hyperthermia (WBH) has an adverse effect on the nervous system and neurophysiological performance. In the present study, we examined whether short-duration whole-body immersion in 45°C water (HWI-45°C), which produces a strong neural and temperature flux without inducing WBH, can increase or impair neurophysiological performance in humans.

Methods: Fifteen men (aged 25 ± 6 years) were enrolled in this study and participated in three experiments: 1) a brief (5-min) immersion of the whole body in 37°C water (WI-37°C); 2) a brief (5-min) HWI-45°C; and 3) a control trial in a thermoneutral condition at an ambient temperature of 24°C and 60% relative humidity. Before and after the immersions, neuromuscular function (electromyographic activity, reflexes, electrically and voluntary induced torque production, voluntary muscle activation level) were tested. To provoke central inhibition, the participants performed a sustained 2-min maximal voluntary contraction (MVC).

Results: Thermophysiological strain was greater after HWI-45°C than after WI-37°C. Electrophysiological modulations of motor drive transmission and peripheral modulations of muscle contractility properties in response to HWI-45°C seemed to have little effect on central activation of the exercising muscles and no effect on MVC production.

Conclusion: Although exposure to acute noxious heat was effective in evoking neuromuscular excitability, the increases in core temperature (∼0.2°C) and muscle temperature (∼0.6°C) did not induce moderate or severe WBH. These changes did not seem to affect central structures; that is, there were no additional increases in central and/or peripheral fatigue during a sustained 2-min MVC.
Real-time metabolic monitoring under exhaustive exercise and evaluation of ventilatory threshold by breathomics: Independent validation of evidence and advances
Authors: Pugliese Giovanni , Trefz Phillip , Weippert Matthias , Pollex Johannes , Bruhn Sven , Schubert Jochen K. , Miekisch Wolfram , Sukul Pritam
Published: August 2022

Breath analysis was coupled with ergo-spirometry for non-invasive profiling of physio-metabolic status under exhaustive exercise. Real-time mass-spectrometry based continuous analysis of exhaled metabolites along with breath-resolved spirometry and heart rate monitoring were executed while 14 healthy adults performed ergometric ramp exercise protocol until exhaustion. Arterial blood lactate level was analyzed at defined time points. Respiratory-cardiac parameters and exhalation of several blood-borne volatiles changed continuously with the course of exercise and increasing workloads. Exhaled volatiles mirrored ventilatory and/or hemodynamic effects and depended on the origin and/or physicochemical properties of the substances. At the maximum workload, endogenous isoprene, methanethiol, dimethylsulfide, acetaldehyde, butanal, butyric acid and acetone concentrations decreased significantly by 74, 25, 35, 46, 21, 2 and 2%, respectively. Observed trends in exogenous cyclohexadiene and acetonitrile mimicked isoprene profile due to their similar solubility and volatility. Assignment of anaerobic threshold was possible via breath acetone. Breathomics enabled instant profiling of physio-metabolic effects and anaerobic thresholds during exercise. Profiles of exhaled volatiles indicated effects from muscular vasoconstriction, compartmental distribution of perfusion, extra-alveolar gas-exchange and energy homeostasis. Sulfur containing compounds and butyric acid turned out to be interesting for investigations of combined diet and exercise programs. Reproducible metabolic breath patterns have enhanced scopes of breathomics in sports science/medicine.
Photosynthetic product allocations of Pinus massoniana seedlings inoculated with ectomycorrhizal fungi along a nitrogen addition gradient
Authors: Pengfei Sun , Yafei Shen , Lijun Wang , Tian Chen , Meng Zhang , Wenfa Xiao , Ruimei Cheng
Published: August 2022

Quantifying the allocation of photosynthetic products among different carbon (C) pools is critical for understanding and predicting plant C turnover response to climate change. A field experiment with ectomycorrhizal fungi (EMF) and nitrogen (N) was established to investigate the effects on allocation of photosynthetic products in Pinus massoniana (Lamb.) seedlings given increased N deposition. Seedlings were subjected to N addition and symbiosis with EMF, and the short-term allocation of a 13C photosynthetic pulse into leaves, branches, stems, roots, and soil was traced. Photosynthetic rate and root respiration were measured. It was found that N addition changed the allocation pattern of photosynthetic products in various organs of P. massoniana. Furthermore, N addition, mycorrhizal symbiosis, and interaction of N and EMF, all increased the amount of C produced by photosynthesis. N application less than 60 kg N hm–1 a–1 could promote the transfer and allocation of photosynthetic products in P. massoniana organs, which peaks at 60 kg N hm–1 a–1, and the highest N treatment began to decrease at 90 kg N hm–1 a–1. EMF inoculation could expand the absorption area of plant roots to obtain more nutrients and synthesize more C and N compounds for promoting the growth of itself and the host plant, improving the net photosynthetic rate and the distribution of C produced by photosynthesis in various organs. This forms a benign C and N cycle, thereby reducing the effect of high N addition on plants. The optimal N addition concentration was 60 kg N hm–1 a–1, and the optimal EMF was Pt, which provides a theoretical basis for inoculating EMF during increasing N deposition in the future climate change scenario. This enables plants to distribute more photosynthetic products to their roots, thus affecting their own C distribution for promoting growth.
Field rice panicle detection and counting based on deep learning
Authors: Wang Xinyi , Yang Wanneng , Lv Qiucheng , Huang Chenglong , Liang Xiuying , Chen Guoxing , Xiong Lizhong , Duan Lingfeng
Published: August 2022

Panicle number is directly related to rice yield, so panicle detection and counting has always been one of the most important scientific research topics. Panicle counting is a challenging task due to many factors such as high density, high occlusion, and large variation in size, shape, posture et.al. Deep learning provides state-of-the-art performance in object detection and counting. Generally, the large images need to be resized to fit for the video memory. However, small panicles would be missed if the image size of the original field rice image is extremely large. In this paper, we proposed a rice panicle detection and counting method based on deep learning which was especially designed for detecting rice panicles in rice field images with large image size. Different object detectors were compared and YOLOv5 was selected with MAPE of 3.44% and accuracy of 92.77%. Specifically, we proposed a new method for removing repeated detections and proved that the method outperformed the existing NMS methods. The proposed method was proved to be robust and accurate for counting panicles in field rice images of different illumination, rice accessions, and image input size. Also, the proposed method performed well on UAV images. In addition, an open-access and user-friendly web portal was developed for rice researchers to use the proposed method conveniently.
Multiteam Systems in Long Duration Exploration Missions: A Qualitative Analysis of Key Characteristics and Challenges
Authors: Verhoeven Dana C. , Kramer William S. , Shuffler Marissa L.
Published: August 2022

Given the unprecedented environment of long duration space exploration (LDSE), success simply cannot occur without the coordinated efforts of multiple teams, both in flight and on the ground. These multiteam systems (MTSs) are needed to achieve the complex and dynamic tasks of spaceflight missions that will be longer and more uncertain than any previously experienced. Accordingly, research is limited in terms of how to best coordinate these teams and their dynamics—and in particular, how to best prepare LDSE teams to work across time and space effectively. To begin to address these critical questions systematically, qualitative data was extracted from a series of ten interviews with experts in spaceflight and long duration analog environments. Using thematic analysis techniques, we identified several consistent themes for affective, behavioral, and cognitive elements of teamwork occurring within and between teams. We examine each of these in detail, to identify the dynamics of what is currently known and where research needs to go to provide guidance for spaceflight organizations as well as others attempting to successfully implement MTSs in novel, complex environments.
The relationship between childhood trauma and post-traumatic growth among college students: The role of acceptance and positive reappraisal
Authors: Quan Lijuan , Lü Bijun , Sun Jialei , Zhao Xintong , Sang Qingsong
Published: August 2022

Objective

To examine the relationship among childhood trauma, acceptance, positive reappraisal and post-traumatic growth (PTG) among college students.

Methods

Research participants were selected by random cluster sampling. 1,028 college students (62.6% female, 30.5% only-children) from 8 universities were investigated using manuscript-pencil survey versions of Childhood Trauma Questionnaire-Short Form (CTQ-SF), Cognitive emotion regulation questionnaire–Chinese version (CERQ-C) and Post traumatic Growth Inventory (PTGI).

Results

Traumatic childhood experience significantly negatively predicts post traumatic growth in college students. Exposure to traumatic experiences in childhood can directly negatively predict post-traumatic growth and indirectly positively predict post traumatic growth via acceptance.

Conclusion

Acceptance plays a mediating role between childhood traumatic experience and post traumatic growth. The mediating effect of acceptance is moderated by the positive reappraisal. When individuals have a lower level of positive reappraisal, the mediating effect between traumatic experience and post traumatic growth is significant. Several clinical implications for clinical psychology and psychological intervention are highlighted. Starting with changing individual cognition and helping individuals adopt positive cognitive emotion regulation strategies can help individuals actively reevaluate traumatic experience, so as to gain better and faster counseling results.
The relationship between resilience, anxiety, and depression in Chinese collegiate athletes
Authors: Lyu Chengjie , Ma Rong , Hager Ronald , Porter Dave
Published: August 2022

Anxiety disorder is becoming increasingly prevalent across college campuses, particularly among collegiate athletes who face different stressors and challenges than their non-athlete peers. Anxiety and depression are well documented comorbidities. Furthermore, the number of Chinese collegiate athletes suffering from anxiety and depression is increasing. Psychologists have recently begun to take a more prophylactic approach to defend against these disorders through resilience education. Resilience education focuses on developing greater capacity to cope when mental and emotional challenges arise. The purpose of this study was to analyze the relationship between resilience, depression, and anxiety in Chinese collegiate athletes. This study provides additional evidence of the value of incorporating an effective resilience educational module amongst collegiate athletes.

Research design

The study employed a survey research design to explore the complex relationship between depression, anxiety, and resilience for collegiate student athletes.

Data analysis strategy

Structural Equation Modeling (SEM) was employed to account for any measurement error between the observed items (responses on the instruments) and the latent variables (anxiety, depression, and resilience). The theoretical hypothesized relationship for this study is an inverse relationship between anxiety and depression, on the one hand, and resilience on the other hand. The SEM statistical analysis from this study supported this theoretical model.

Effective sample size

599 collegiate athletes from six different universities in the province of Guangdong, China P.R. participated in this study.
Time management disposition and relevant factors among new nurses in Chinese tertiary hospitals: A cross-sectional study
Authors: Xie Jianfei , Wu Xiaoqi , Li Jie , Li Xiaolian , Xiao Panpan , Wang Sha , Zhong Zhuqing , Ding Siqing , Yan Jin , Li Lijun , Cheng Andy S. K.
Published: August 2022

Introduction

New nurses struggled with time management, which was a prominent theme in safety care for patients. However, the transition training of time management for new nurses was complicated and ignored by clinical managers. The purpose of this study was to understand the level of new nurses’ TMD from a nationwide perspective and detect the influencing factors of the TMD.

Materials and methods

A cross-sectional study design with a stratified sampling method was sampled in China. Six hundred and seventy new nurses within the first year of employment were recruited. New nurses’ time management disposition, job stressors, self-efficacy, clinical communication competence, and safety behavior were measured by corresponding scales.

Results

New nurses showed the best sense of time’s value, followed by the sense of time efficacy and time monitoring view for time management disposition. The related factors of time management disposition were communication skills, safety behavior, job stressors, and being without a preceptor. New nurses’ time management disposition was at a moderate level and they performed worse in time allocation. The highest education, with or without a preceptor, the experience of part-time jobs, and class cadre were significantly influencing the time management disposition of new nurses.

Conclusion

Nursing managers should pay attention to new nurses’ time management disposition. Reducing the job stressors, improving communication ability, and safe behavior were important measures to improve the time management disposition.
Nutrition literacy and its related demographic factors among workers of Taraz Steel company, Chaharmahal and Bakhtiari, Iran
Authors: Yarmohammadi Parastoo , Morowatisharifabad Mohammad Ali , Rahaei Zohreh , Khayyatzadeh Sayyed Saeid , Madadizadeh Farzan
Published: August 2022

Background

Nutrition is critical to prevent some chronic diseases. Nutrition literacy refers to ability to gain, understand and evaluate nutrition facts to choose appropriate foods. Nutrition literacy has recently drawn the attention of professionals with respect to health promotion. The purpose of this study was to investigate nutrition literacy and potentially related demographic factors among workers of a steel company in Chaharmahal and Bakhtiari province, southwest Iran.

Methods

In this descriptive-analytical study in 141 workers of Taraz Steel company in 2021, participants were selected by convenience sampling and a self-report nutrition literacy scale nativized to Iranians was used to collect data. Data were analyzed by SPSS 22 using Mann-Whitney U test, Kruskal-Wallis test and Spearman's correlation coefficient.

Results

About 75% of workers had adequate nutrition literacy and around 24% inadequate nutrition literacy. The highest percentage of mean score was obtained for determination of food groups (85.4%) and the lowest for calculation of food units (47%). The mean score of nutrition literacy was significantly higher in people with higher education (P = 0.020). Also, people with adequate monthly salary attained a higher mean score on determination of food groups (P = 0.021) and higher overall nutrition literacy (P = 0.003) compared to other people. No relationship was observed between nutrition literacy and body mass index as well.

Conclusion

Most workers have adequate nutrition literacy but their scores on calculation of food units are relatively low. It is essential for policymakers to collect information on the level of nutrition literacy in different populations, especially Iranian workers, to reduce the prevalence of nutrition-related chronic diseases.
In the eyes of the beholder: Race, place and health
Authors: Velasquez Alfredo J. , Douglas Jason A. , Guo Fangqi , Robinette Jennifer W.
Published: August 2022

Racial and ethnic health disparities are fundamentally connected to neighborhood quality. For example, as a result of historical systemic inequities, racial and ethnic minorities are more likely to live in neighborhoods with signs of physical disorder (e.g., graffiti, vandalism), and physically disordered environments have been noted to associate with increased risk for chronic illness. Degree of exposure to neighborhood disorder may alter peoples' perception of their neighborhoods, however, with those most exposed (e.g., historically marginalized racial/ethnic groups) perhaps perceiving less threat from signs of neighborhood disorder. The purpose of the present study was to examine the complex interrelationships between people and place by investigating whether exposure to neighborhood physical disorder relates to residents' (1) perceptions of neighborhood safety and (2) perceptions of their health, and (3) examining whether these links vary by race/ethnicity. Using 2016–2018 Health and Retirement Study (HRS) data, a representative sample of US adults aged 51 years and older (n = 9,080, mean age 68 years), we conducted a series of weighted linear regressions to examine the role of neighborhood disorder in relation to both perceived neighborhood safety and self-rated health. Results indicated that greater neighborhood physical disorder was statistically significantly related to feeling less safe among non-Hispanic Whites and Hispanics, but not non-Hispanic Blacks. Regarding self-rated health, neighborhood physical disorder was statistically significantly related to poorer health among all racial/ethnic groups. These findings suggest that, despite differential interpretation of neighborhood disorder as a threat to safety, this modifiable aspect of peoples' environment is related to poor health regardless of one's race/ethnicity.
Identifying conditions for a third dose intention of COVID-19 vaccination in college students: A fuzzy-set qualitative comparative analysis
Authors: Gao Wanqin , Zhang Yulin , Yin Gang
Published: August 2022

Background

During the pandemic, a third dose of the COVID-19 vaccine effectively reduces the proportion of severe cases in those infected, but vaccine hesitancy impedes this reasonable prevention method. Epidemic control in China is being tested due to the large population base, especially in crowded places like college campuses. This study aimed to explore the configuration paths of psychological antecedents for college students to receive a third COVID-19 vaccine.

Methods

An anonymous cross-sectional survey was carried out in five universities in Wuhan using convenience sampling. A long version of the 5C 7-point Likert scale was used to measure college students' intention and psychological antecedents on the third dose of the COVID-19 vaccine. A fuzzy-set Qualitative Comparative Analysis (QCA) approach was performed to explore the configuration of conditions to the vaccination willingness.

Results

31.67% of respondents surveyed did not receive their third dose of the COVID-19 vaccine. The score of intention to get the vaccine for college students who did not receive the booster vaccine was 4.93 (±1.68). Average scores of 5.19 (±1.24), 4.35 (±1.75), 4.02 (±1.45), 5.84 (±1.23), and 4.61(±1.32) were reported for confidence, complacency, constraints, calculation, and collective responsibility in them. QCA showed high confidence and collective responsibility playing a central role in third dose vaccination intention. Meanwhile, low confidence and collective responsibility are the core conditions of low vaccination willingness.

Conclusion

Eliminating vaccine hesitancy necessitates focusing on the psychological antecedents of vaccination intentions to identify critical targets for policy and interventions. This study identified trust and collective responsibility are core elements of the psychological antecedents of college students' intention to receive the booster vaccine for COVID-19. To achieve herd immunity as soon as possible, health administration and campus can start with vaccine confidence-building and collective responsibility cultivation to take appropriate actions and measures to improve coverage of the booster vaccination.
Effective leaders(hip) in community-academic health partnership projects: An inductive, qualitative study
Authors: Chak Choiwai Maggie , Carminati Lara
Published: August 2022

To deepen our understanding of how project leaders can lead effectively in different community-academic health partnerships (CAHPs), we conducted an inductive, qualitative study through semi-structured interviews (N = 32) and analyzed the data with Grounded Theory approaches. By presenting a process model illustrating the cycle of effective leaders(hip) in CAHP projects, we contribute to the literature on CAHP, leadership development, and complexity leadership theory in three ways. Firstly, the model depicts the strategies enabling leaders to navigate typical project challenges and perform leadership tasks effectively. Secondly, we distill four beneficial qualities (i.e., adopting a proactive attitude, having an open and adaptive mindset, relying on peer learning and support, and emphasizing self-growth and reflexivity) which CAHP project leaders require to develop themselves into effective leaders. Thirdly, we illustrate leaders' dynamic developmental logics and processes of effective leadership and their contributions to better project functioning in diverse CAHPs.
Optimizing bowel preparation for colonoscopy: A cross-sectional study of the Chinese population
Authors: Luo Li , Liu Yuan , Zhang Lingling , Lai Yihuan , Li Yansheng , Liu Kejia , Gong Houwu , Jiang Dapeng , Wang Erchuan
Published: August 2022

Background

The quality of bowel preparation is an important factor in the success of colonoscopy. However, the quality of bowel preparation is often affected by multiple factors. The main objective of this study was to explore the specific factors that affect the quality of bowel preparation.

Methods

Patients were consecutively recruited from the gastroenterology department in Union Hospital, Tongji Medical College, Huazhong University of Science and Technology in Wuhan from May 2018 to December 2018. All patients were undergoing colonoscopy. Bowel preparation was evaluated by the Ottawa Bowel preparation Scale (OBPS) and all patients were categorized into 2 groups according to the OBPS. Multivariate analysis was conducted to identify the factors associated with bowel preparation quality.

Results

A total of 910 patients were included in the analysis with an average age of 48.62 ± 13.57 years. Patient source (P < 0.001) and the preparation method (P = 0.029) were correlated with OBPS adequacy. In addition, after stratified by age, preparation method (P = 0.022) was a significant factor among patients under 50 years old; whereas waiting time (P = 0.005) was a significant factor among patients over 50 years old.

Conclusion

Bowel preparation should be tailored based on the age of the patients to determine the most appropriate plan, including the most appropriate waiting time and the most appropriate purgative combination. Doctors should also focus more on the quality of bowel preparation in inpatients, who are more likely than outpatients to have an inadequate bowel preparation.
Assessing Crop and Corn Silage Profile in Beef Cattle Farms in Southern Brazil: Ten Years’ Results
Authors: Horst Egon Henrique , Neumann Mikael
Published: August 2022

This work aimed to investigate corn crop management practices and the quality of corn silage in beef cattle farms in southern Brazil during ten years of sampling. A total of 63 farms, located in 16 municipalities in Paraná state, Brazil, participated in the study of corn crop characterization, while the corn silages were obtained from 65 farms located in 18 municipalities in the same state. A total of 49 corn hybrids were identified, with a preference for early cycle hybrids (E) and semi-hard grain type (SH). The dataset was obtained between 2011 and 2020, and the average cycle of the identified hybrids ranged from 150 ± 14 days in 2012 to 134 ± 12 days in 2019, with a maximum amplitude of 182 days in 2012 and a minimum of 99 days in 2019 and 2020. The DM content of the plant at harvest ranged from 40.1% to 45.9% between the average of the years evaluated, with an average dry biomass yield of 25 t ha−1. The analyzed silages had average levels of aNDF and ADF within the expectation (45.6% and 26.2%, respectively) but with CP content below that commonly observed in corn silages (6.9%) due to the stage of advanced maturity at harvest. Based on this monitoring, we emphasize that the production of corn silage on beef cattle farms in southern Brazil faces specific challenges, which include outsourcing the provision of harvesting services. Above all, it was clear that there were no significant advances in the results obtained over ten years, raising concerns about interventions in the process.
The Effect of Paracrine Factors Released by Irradiated Peripheral Blood Mononuclear Cells on Neutrophil Extracellular Trap Formation
Authors: Klas Katharina , Ondracek Anna S. , Hofbauer Thomas M. , Mangold Andreas , Pfisterer Karin , Laggner Maria , Copic Dragan , Direder Martin , Bormann Daniel , Ankersmit Hendrik Jan , Mildner Michael
Published: August 2022

Neutrophil extracellular trap (NET)-formation represents an important defence mechanism for the rapid clearance of infections. However, exaggerated NET formation has been shown to negatively affect tissue-regeneration after injury. As our previous studies revealed the strong tissue-protective and regenerative properties of the secretome of stressed peripheral blood mononuclear cells (PBMCsec), we here investigated the influence of PBMCsec on the formation of NETs. The effect of PBMCsec on NET formation was assessed ex vivo in ionomycin stimulated neutrophils derived from healthy donors using flow cytometry, image stream analysis, and quantification of released extracellular DNA. The effect of PBMCsec on molecular mechanisms involved in NET formation, including Ca-flux, protein kinase C activity, reactive oxygen species production, and protein arginine deiminase 4 activity, were analysed. Our results showed that PBMCsec significantly inhibited NET formation. Investigation of the different biological substance classes found in PBMCsec revealed only a partial reduction in NET formation, suggesting a synergistic effect. Mechanistically, PBMCsec treatment did not interfere with calcium signalling and PKC-activation, but exerted anti-oxidant activity, as evidenced by reduced levels of reactive oxygen species and upregulation of heme oxygenase 1 and hypoxia inducible-factor 1 in PBMCsec-treated neutrophils. In addition, PBMCsec strongly inhibited the activation of protein arginine deiminase 4 (PAD4), ultimately leading to the inhibition of NET formation. As therapeutics antagonizing excessive NET formation are not currently available, our study provides a promising novel treatment option for a variety of conditions resulting from exaggerated NET formation.
Plant-Derived Bioactive Compounds in Colorectal Cancer: Insights from Combined Regimens with Conventional Chemotherapy to Overcome Drug-Resistance
Authors: Gavrilas Laura Ioana , Cruceriu Daniel , Mocan Andrei , Loghin Felicia , Miere Doina , Balacescu Ovidiu
Published: August 2022

Acquired drug resistance represents a major clinical problem and one of the biggest limitations of chemotherapeutic regimens in colorectal cancer. Combination regimens using standard chemotherapeutic agents, together with bioactive natural compounds derived from diet or plants, may be one of the most valuable strategies to overcome drug resistance and re-sensitize chemoresistant cells. In this review, we highlight the effect of combined regimens based on conventional chemotherapeutics in conjunction with well-tolerated plant-derived bioactive compounds, mainly curcumin, resveratrol, and EGCG, with emphasis on the molecular mechanisms associated with the acquired drug resistance.
β2-Adrenergic Receptor Expression and Intracellular Signaling in B Cells Are Highly Dynamic during Collagen-Induced Arthritis
Authors: Honke Nadine , Wiest Clemens J. , Pongratz Georg
Published: August 2022

The sympathetic nervous system (SNS) has either a pro-inflammatory or anti-inflammatory effect, depending on the stage of arthritis. In the past, treatment of arthritic B cells with a β2-adrenergic receptor (β2-ADR) agonist has been shown to attenuate arthritis. In this study, the expression and signaling of β2-ADR in B cells during collagen-induced arthritis (CIA) were investigated to provide an explanation of why only B cells from arthritic mice are able to improve CIA. Splenic B cells were isolated via magnetic-activated cell sorting (MACS). Adrenergic receptors on B cells and intracellular β2-ADR downstream molecules (G protein-coupled receptor kinase 2 (GRK-2), β-Arrestin 2, p38 MAPK, extracellular signal-regulated kinase 1/2 (ERK1/2) and cAMP response element-binding protein (CREB)) were analyzed at different time points in naïve and arthritic B cells with and without stimulation of β2-ADR agonist terbutaline by flow cytometry. β2-ADR-expressing B cells increase during CIA without a change in receptor density. Moreover, we observed a profound downregulation of GRK-2 shortly after induction of arthritis and an increase in β-Arrestin 2 only at late stage of arthritis. The second messengers studied (p38, ERK1/2 and CREB) followed a biphasic course, characterized by a reduction at onset and an increase in established arthritis. Stimulation of CIA B cells with the β-ADR agonist terbutaline increased pp38 MAPK independent of the timepoint, while pERK1/2 and pCREB were enhanced only in the late phase of arthritis. The phosphorylation of p38 MAPK, ERK1/2 and CREB in the late phase of arthritis was associated with increased IL-10 produced by B10 cells. The change of β2-ADR expression and signaling during sustained inflammation might be an integral part of the switch from pro- to anti-inflammatory action of sympathetic mechanisms in late arthritis.
Testicular Germ Cell Tumours—The Role of Conventional Ultrasound
Authors: Belfield Jane , Findlay-Line Charlotte
Published: August 2022

Testicular tumours are the most common tumours found in young males and germ cell tumours account for 95% of testicular tumours. Ultrasound is the first-line radiological investigation for imaging of the testis. This article outlines how to undertake an ultrasound examination, including optimal patient position, scanning technique and imaging parameters. Classification of germ cell tumours is provided, and salient imaging features of different tumours are described. Difficulties and pitfalls of ultrasound are described, including tumours found after a trauma presentation, orchitis causing diagnostic difficulties and imaging of small testicular lesions. Other uses of ultrasound are outlined, including looking for a primary testicular tumour following the discovery of retroperitoneal lymph nodes, imaging when tumour-makers increase, local recurrence in the scrotum, and for solid organ biopsy in metastatic disease. Conclusion: Ultrasound remains the first-line of investigation for imaging of the testis, and conventional ultrasound still plays a large role in imaging. On ultrasound alone, accurate morphological characterisation of tumours remains a challenge, despite the imaging features that can be seen in different tumour types. Therefore, histology following orchidectomy of a germ cell tumour remains the gold standard for accurate tumour characterisation.
Are Columnar Cell Lesions the Earliest Non-Obligate Precursor in the Low-Grade Breast Neoplasia Pathway?
Authors: Strickland Sarah , Turashvili Gulisa
Published: August 2022

Columnar cell lesions (CCLs) of the breast comprise a spectrum of morphologic alterations of the terminal duct lobular unit involving variably dilated and enlarged acini lined by columnar epithelial cells. The World Health Organization currently classifies CCLs without atypia as columnar cell change (CCC) and columnar cell hyperplasia (CCH), whereas flat epithelial atypia (FEA) is a unifying term encompassing both CCC and CCH with cytologic atypia. CCLs have been increasingly recognized in stereotactic core needle biopsies (CNBs) performed for the assessment of calcifications. CCLs are believed to represent the earliest non-obligate precursor of low-grade invasive breast carcinomas as they share molecular alterations and often coexist with entities in the low-grade breast neoplasia pathway. Despite this association, however, the risk of progression of CCLs to invasive breast carcinoma appears low and may not exceed that of concurrent proliferative lesions. As the reported upgrade rates of pure CCL/FEA when identified as the most advanced high-risk lesion on CNB vary widely, the management of FEA diagnosed on CNB remains controversial. This review will include a historical overview of CCLs and will examine histologic diagnostic criteria, molecular alterations, prognosis and issues related to upgrade rates and clinical management.
Annona cherimola Mill. Leaf Extracts Affect Melanoma Cells Growth and Progression
Authors: Iacopetta Domenico , Fazio Alessia , La Torre Chiara , Barbarossa Alexia , Ceramella Jessica , Francomano Fabrizio , Saturnino Carmela , El-Kashef Hussein , Alcaro Stefano , Sinicropi Maria Stefania
Published: August 2022

Cancer represents one of the major causes of mortality worldwide; indeed, 19.3 million new cases and almost 10.0 million deaths were estimated last year. Among the different type of cancers, malignant melanoma represents the most aggressive and deadly skin cancer. Unfortunately, the long-term efficacy of melanoma treatments is limited by the lack of clinical efficacy, onset of side effects and resistance. The latter is a major obstacle for the success of the melanoma therapy; thus, the exploration of new potent and safer anticancer agents is of great importance. Recently, numerous plant species, used for therapeutic purposes and containing various non-toxic nutraceuticals have been widely studied. Herein, we investigated the antioxidant and anticancer properties on melanoma cells of the ethanolic, methanolic and aqueous Annona cherimola leaf extracts (ACE, ACM and ACW, respectively). The ethanolic extract showed higher anticancer activity, mostly against the malignant A2058 melanoma cell line (IC50 = 5.6 ± 0.8 ng/mL), together with a very low activity on the normal cells. It blocks the melanoma cells migration process, and induces a clear disorganization of cytoskeleton, triggering cell apoptosis. Finally, some bioactive compounds were identified in the studied extracts.
Updating Distribution, Ecology, and Hotspots for Three Amphibian Species to Set Conservation Priorities in a European Glacial Refugium
Authors: Bernabò Ilaria , Cittadino Viviana , Tripepi Sandro , Marchianò Vittoria , Piazzini Sandro , Biondi Maurizio , Iannella Mattia
Published: August 2022

The Calabrian Peninsula (Southern Italy) has acted as a glacial refugium and is now considered a hotspot for the genetic diversity of several species. Even if it hosts the highest diversity of many Italian endemic amphibian species, the distribution of some of these needs an update to address conservation measures. We took advantage of a vast dataset for three Italian species (Bombina pachypus, Salamandrina terdigitata, Triturus carnifex), two of which are endemic, deriving from a 40-year field surveys dataset (1982–2022), to update their distribution and basic ecological requirements. We evaluated changes in their distribution, projecting them on a broader spatial scale through a kernel density estimation, inferring statistically-significant hotspots using Corine Land Cover patches, and assessing the protected areas’ coverage. We confirmed that Pollino, Catena Costiera, Sila and Aspromonte massifs are the main statistically-significant hotspots. Kernel densities showed a diversified pattern of gains/losses, sometimes overlapping, depending on the species. The whole outcomes obtained allow us to pinpoint specific areas where effective conservation measures need to be applied. Ousr findings reveal that local-scale monitoring and management should be planned, especially within the existing nationally-designated protected areas, which have been shown to protect far less with respect to the Natura 2000 sites.
Comparison of Selected Mathematical Programming Models Used for Sustainable Land and Farm Management
Authors: Moulogianni Christina
Published: August 2022

The aim of this study is to compare three mathematical programing models used for sustainable land and farm management. The sample for the comparison was 219 agricultural holdings participating as beneficiaries of the measure ‘Modernization of agricultural holdings’ in the Rural Development Plan at the Region of Central Macedonia in Greece. Using the crop plan of the agricultural land of these farms the mathematical programming models calculate the optimum solution under different and conflicting goals. The results of the methodologies of Linear Programming (LP), of Positive Mathematical Programming (PMP) and Weighted Goal Programming (WGP), are compared in terms of the proposed agricultural land changes. The sustainability of farms is measured with the use of eleven economic, social, and environmental indicators. Each model has some unique advantages and disadvantages that can enable it to be implemented in particular situations. In the conclusions to this research the characteristics of each model are highlighted.
The Interaction between Urban and Rural Areas: An Updated Paradigmatic, Methodological and Bibliographic Review
Authors: Delgado-Viñas Carmen , Gómez-Moreno María-Luisa
Published: August 2022

The relationships and interactions between rural and urban spaces have long been of interest in the territorial sciences. However, the approaches taken to these questions have evolved in line with the changing characteristics of the two types of territories, reflecting new relationships and structures. From these premises, we update the concept of rural–urban interaction by means of an extensive bibliographic review, which, among other results, highlights: (1) the profound change that has taken place in recent years in rural–urban interaction through processes such as de-agrarianisation, the tertiarisation of the economy and improvements in transport and communication infrastructures; (2) the resulting obsolescence of earlier typologies and procedures focused on discrimination between rural and urban environments, rather than on the interaction between them; (3) the difficulty of establishing valid, widely applicable typologies, given the profound differences in terms of (a) the scale and content of the statistics available in each country and (b) the territorial background in terms of economic functions and the characteristics, ancient and modern, of human settlement; (4) the predominance of an urban-centric approach, to the detriment of more traditional rural functions, such as agriculture, the importance of which is diluted by its low relative weight in terms of employed population and contribution to GDP. Consideration of these findings leads us to propose a new approach to the question of rural–urban interaction, reflecting the multifunctionality of rural spaces, and we identify useful areas for future research.
CFD Investigation on Oil Injection Lubrication of Meshing Spur Gears via Lattice Boltzmann Method
Authors: Hu Xiaozhou , Li Pengfei , Quan Can , Wang Jianing
Published: August 2022

The meshless Lattice Boltzmann Method (LBM) is introduced and employed to solve the complex two-phase flow problem of jet lubrication of meshing spur gears. Computational fluid dynamics (CFD) simulations based on LBM are carried out using the model of an oil jet impacting rotating gear presented by available experiments, which reveals how the liquid column is broken throughout the tooth tip cutting off the oil jet. Typical oil flow phenomena obtained by simulations are compared with experiments, demonstrating good qualitative agreement, which validates the feasibility of LBM to simulate the air–oil–structure interaction problems involved in the jet lubrication of spur gears. A three-dimensional (3D) simulation model of a spur gear pair lubricated by an oil jet is established, and simulations with different operating conditions are conducted. The evolution process of the oil jet while injecting into the meshing zone is captured, and the effects of jet velocities, jet heights and jet angles on the lubrication performance are investigated.
Design and Mechanical Behavior of a Custom Adapter for Dimensional Stone Mining
Authors: Virág Zoltán , Szirbik Sándor
Published: August 2022

The paper deals with the development of a dimensional rock sawing machine to optimize the production of small-volume dimensional stone products in limestone quarries by re-thinking the use of production and transport equipment. A low-volume mining production and transportation adapter to be developed for both production and transport can reduce the number of mining machines to be used in the mine. After cutting around the block stone, the rock material can be moved with the help of the machine. Cutting tests were performed to determine the winning properties of the limestone. The first objective of the paper was to calculate the cutting forces and cutting powers and eliminate the failure possibilities and vibrations. In this study, the eigenvalue extraction used to calculate the natural frequencies and mode shapes was based on the Lanczos iteration methods using the Abaqus software to identify any potentially dangerous frequencies. By taking the load cases from modelling of the extraction of limestone and the transport of block stones, the deformation and strength properties of the adapter were also examined using FE analysis for stress/displacement analysis. Drawing the conclusion from finite element analysis, the construction of the adapter can be developed and checked. The results can be used with great confidence, so the main dimensions of the concept serve as the basis for the dimensions of the adapter.