bluuebunny/crossref_metadata_2025 huggingface dataset:
Advances in Adjoint Functions of Connection Number in Water Resources Complex Systems: A Systematic Review
Authors: Zhou Liangguang , Jin Juliang , Zhou Rongxing , Cui Yi , Wu Chengguo , Zhou Yuliang , Dai Shibao , Zhang Yuliang
Published: April 2024

The adjoint function of connection number has unique advantages in solving uncertainty problems of water resource complex systems, and has become an important frontier and research hotspot in the uncertainty research of water resource complex problems. However, in the rapid evolution of the adjoint function, some problems greatly limit the application of the adjoint function in the research of water resources. Therefore, based on bibliometric analysis, development, practical application issues, and prospects of the hot directions are analyzed. It is found that the development of the connection number of water resource set pair analysis can be divided into three stages: (1) relatively sluggish development before 2005, (2) a period of rapid advancement in adjoint function research spanning from 2005 to 2017, and (3) a subsequent surge post-2018. The introduction of the adjoint function of connection number promotes the continuous development of set pair analysis of water resources. Set pair potential and partial connection number are the crucial research directions of the adjoint function. Subtractive set pair potential has rapidly developed into a relatively independent and important trajectory. The research on connection entropy is comparatively less, which needs to be further strengthened, while that on adjacent connection number is even less. The adjoint function of set pair potential can be divided into three major categories: division set pair potential, exponential set pair potential, and subtraction set pair potential. The subtraction set pair potential, which retains the original dimension and quantity variation range of the connection number, is widely used in water resources and other fields. Coupled with the partial connection number, a series of new connection number adjoint functions have been developed. The partial connection number can be mainly divided into two categories: total partial connection number, and semi-partial connection number. Among these, the calculation expression and connotation of total partial connection numbers have not yet reached a consensus, accompanied by the slow development of high-order partial connection numbers. Semi-partial connection number can describe the mutual migration movement between different components of the connection number, which develops rapidly. With the limitations and current situation described above, promoting the exploration and application of the adjoint function of connection number in the field of water resources and other fields of complex systems has become the focus of future research.
The Mechanism Study of Fracture Porosity in High-Water-Cut Reservoirs
Authors: Zhang Ning , Yin Daiyin , Cao Guangsheng , Li Tong
Published: April 2024

Many onshore oil fields currently adopt water flooding as a means to supplement reservoir energy. However, due to reservoir heterogeneity, significant differences in permeability exist not only between different reservoirs but also within the same reservoir across different planar orientations. After prolonged fluid flushing in the near-wellbore zone of injection wells, the resulting increased flow resistance between layers exacerbates inefficient and ineffective circulation. A considerable amount of remaining oil is left unexploited in untouched areas, significantly impacting the overall recovery. To investigate the multiscale plugging mechanisms of fracture-dominated pore channels in high-water-cut oil reservoirs and achieve efficient management of fractured large channels, this study explores the formation of the fracture-flushing zone-low saturation oil zone. A physical experimental model with fractures and high-intensity flushing is established to analyze changes in pore structure, mineral composition, residual oil distribution, and other characteristics at different positions near the fractures. The research aims to clarify the mechanism behind the formation of large channels with fracture structures. The results indicate that under high-intensity water flushing, cementing materials are washed away by the flowing water, clay particles are carried to the surface with the injected fluid, and permeability significantly increases, forming high-permeability zones with fracture structures. In the rock interior away from the fracture end, channels, corners, and clustered oil content noticeably decrease, while the content of film-like oil substantially increases, and clay minerals are not significantly washed away. Under strong flushing conditions, the number of residual clay particles near the fracture end is mainly influenced by flow velocity and flushing time; thus, the greater the flushing intensity, the faster the water flow, and the longer the flushing time, the fewer residual clay particles near the fracture end.
Artificial Intelligence-Centric Low-Enthalpy Geothermal Field Development Planning
Authors: Clemens Torsten , Chiotoroiu Maria-Magdalena , Corso Anthony , Zechner Markus , Kochenderfer Mykel J.
Published: April 2024

Low-enthalpy geothermal energy can make a major contribution towards reducing CO2 emissions. However, the development of geothermal reservoirs is costly and time intensive. In particular, high capital expenditures, data acquisition costs, and long periods of time from identifying a geothermal resource to geothermal heat extraction make geothermal field developments challenging. Conventional geothermal field development planning follows a linear approach starting with numerical model calibrations of the existing subsurface data, simulations of forecasts for geothermal heat production, and cost estimations. Next, data acquisition actions are evaluated and performed, and then the models are changed by integrating the new data before being finally used for forecasting and economics. There are several challenges when using this approach and the duration of model rebuilding with the availability of new data is time consuming. Furthermore, the approach does not address sequential decision making under uncertainty as it focuses on individual data acquisition actions. An artificial intelligence (AI)-centric approach to field development planning substantially improves cycle times and the expected rewards from geothermal projects. The reason for this is that various methods such as machine learning in data conditioning and distance-based generalized sensitivity analysis assess the uncertainty and quantify its potential impact on the final value. The use of AI for sequential decision making under uncertainty results in an optimized data acquisition strategy, a recommendation of a specific development scenario, or advice against further investment. This approach is illustrated by applying AI-centric geothermal field development planning to an Austrian low-enthalpy geothermal case. The results show an increase in the expected value of over 27% and a reduction in data acquisition costs by more than 35% when compared with conventional field development planning strategies. Furthermore, the results are used in systematic trade-off assessments of various key performance indicators.
Solar- and Wind-Energy Utilization in the Kingdom of Saudi Arabia: A Comprehensive Review
Authors: Suliman Fakher Eldin M.
Published: April 2024

Utilizing renewable energy (RE) sources can provide a realistic answer to the problem of many nations’ energy needs. This paper reviews the current status of using RE to produce electricity in the Kingdom of Saudi Arabia (KSA). The primary aim of the review is to identify and analyze the solar- and wind-energy utilization, problems, and future trends in KSA while taking into account all relevant aspects, associated development hurdles, and suggested solutions. All involved parties will receive useful results and conclusions from a thorough examination and analysis of the pertinent literature that can assist them in understanding the potential of solar and wind energy, considering all the linked issues.
Landscape Ecological Risk Assessment of Saihanba under the Change in Forest Landscape Pattern
Authors: Kang Jiemin , Yang Jinyu , Qing Yunxian , Lu Wei
Published: April 2024

Examining the Saihanba Mechanical Forest Farm, this study utilized Landsat remote sensing data from 1987, 1997, 2001, 2013, and 2020 to interpret land use from the Support Vector Machine (SVM) method, and to decipher evolving land use patterns over the last four decades. Grounded in landscape ecology theory, an innovative evaluation index for landscape ecological risk was introduced, leading to the delineation of 382 ecological risk evaluation units. Employing landscape pattern indices and a method of spatial autocorrelation, we analyzed the spatial and temporal distribution characteristics and spatial correlation patterns of landscape ecological risk across five distinct periods. Geostatistical approaches were used to explore the driving factors of landscape risk. The results indicate that since 1987, there have been significant changes in land use types, especially in forest landscapes, their proportion increasing from 23.19% to 74.55%. In 1987, the proportion of high-risk areas was 72.30%, but in 2020, high-risk areas had significantly decreased and clustered in specific locations. The landscape ecological risks in each period of the study area showed a positive spatial correlation and tended to gather in space. After comprehensive exploration using a geographic detector, we found that landscape type, temperature, and vegetation coverage are the main risk factors. Among them, landscape type has the greatest impact on the landscape and works together with slope, aspect, and precipitation. In forest farm management, only the adaptation and adjustment of single factors are often paid attention to, while the compound effects of multiple factors are ignored. The results of this study bring important reference value to the operation and development of forest farms.
Effect of Amine, Carboxyl, or Thiol Functionalization of Mesoporous Silica Particles on Their Efficiency as a Quercetin Delivery System in Simulated Gastrointestinal Conditions
Authors: Matadamas-Ortiz Alexis , Pérez-Robles Juan F. , Reynoso-Camacho Rosalía , Amaya-Llano Silvia L. , Amaro-Reyes Aldo , Di Pierro Prospero , Regalado-González Carlos
Published: April 2024

Quercetin (Q) dietary supplements exhibit poor oral bioavailability because of degradation throughout gastrointestinal digestion (GD), which may be overcome using mesoporous silica particles (MSPs) as an oral delivery system (ODS). This study aimed to elucidate the effect of the functionalization of MSPs with amine-(A-MSP), carboxyl-(C-MSP), or thiol-(T-MSP) groups on their efficiency as a quercetin ODS (QODS). The type and degree of functionalization (DF) were used as factors in an experimental design. The Q-loaded F-MSP (F-MSP/Q) was characterized by gas physisorption analysis, loading capacity (LC), and dynamic light scattering and kinetics of Q release at gastric and intestinal pHs. Antioxidant capacity and Q concentration of media containing F-MSP/Q were evaluated after simulated GD. A-MSP showed the highest LC (19.79 ± 2.42%). C-MSP showed the lowest particle size at pH 1.5 or 7.4 (≈200 nm). T-MSP exhibited the maximum Q release at pH 7.4 (11.43%). High DF of A-MSP increased Q retention, regardless of pH. A-MSP preserved antioxidant capacity of Q-released gastric media (58.95 ± 3.34%). Nonetheless, MSP and F-MSP did not protect antioxidant properties of Q released in intestinal conditions. C-MSP and T-MSP showed essential features for cellular uptake and Q release within cells that need to be assessed.
Blood Cell Ratios Unveiled: Predictive Markers of Myocardial Infarction Prognosis
Authors: Jercălău Cosmina Elena , Andrei Cătălina Liliana , Darabont Roxana Oana , Guberna Suzana , Staicu Arina Maria , Rusu Cătălin Teodor , Ceban Octavian , Sinescu Crina Julieta
Published: April 2024

Background: Even if the management and treatment of patients with non-ST-elevation myocardial infarction (NSTEMI) have significantly evolved, it is still a burgeoning disease, an active volcano with very high rates of morbidity and mortality. Therefore, novel management and therapeutic strategies for this condition are urgently needed. Lately, theories related to the role of various blood cells in NSTEMI have emerged, with most of this research having so far been focused on correlating the ratios between various leukocyte types (neutrophil/lymphocyte ratio-NLR, neutrophil/monocyte ratio-NMR). But what about erythrocytes? Is there an interaction between these cells and leukocytes, and furthermore, can this relationship influence NSTEMI prognosis? Are they partners in crime? Methods: Through the present study, we sought, over a period of sixteen months, to evaluate the neutrophil/red blood cell ratio (NRR), monocyte/red blood cell ratio (MRR) and lymphocyte/red blood cell ratio (LRR), assessing their potential role as novel prognostic markers in patients with NSTEMI. Results: There was a statistically significant correlation between the NRR, LRR, MRR and the prognosis of NSTEMI patients. Conclusions: These new predictive markers could represent the start of future innovative therapies that may influence crosstalk pathways and have greater benefits in terms of cardiac repair and the secondary prevention of NSTEMI.
Factors Affecting Abdominal Obesity: Analyzing National Data
Authors: Kim Gwihyun , Woo Hyekyung , Ji Young-A
Published: April 2024

The purpose of this study is to understand the factors affecting abdominal obesity. A secondary data analysis was conducted to analyze 5262 individuals’ data from the 2020 Korea National Health and Nutrition Examination Survey. The prevalence of obesity was slightly higher in men than women, while abdominal obesity was more prevalent in women. A higher correlation with obesity was observed in young and middle-aged individuals, unmarried individuals, urban residents, those with good subjective health, low-stress perception, moderate alcohol consumption, nonsmokers, regular aerobic exercisers, and those getting more than seven hours of sleep. In contrast, middle-aged and elderly individuals, married individuals, rural residents, those with an elementary school or lower education level, those with low-to-moderate income, those with fair or poor subjective health, high stress perception, nondrinkers, smokers, nonregular aerobic exercisers, and those getting less than seven hours of sleep had a higher correlation with abdominal obesity. Health education suggests that everyone should maintain healthy lifestyle habits, such as getting sufficient sleep, exercise, smoking cessation, and moderate drinking. Specifically, diverse health management support focusing on population groups with demographic factors related to the risk of obesity and abdominal obesity is necessary.
Length of Hospitalization and Mortality among Stroke Patients before and after the Implementation of a Specialized Unit: A Retrospective Cohort Study Using Real-World Data from One Reference Hospital in Southern Brazil
Authors: Poll Marcia , Martins Rodrigo Targa , Anschau Fernando , Jotz Geraldo Pereira
Published: April 2024

Stroke constitutes a significant global cause of mortality and disability. The implementation of stroke units influences hospital quality indicators, guiding care management. We aimed to compare hospital length of stay (LOS), in-hospital mortality, and post-discharge mortality between stroke patients admitted in the pre- and post-implementation periods of a stroke unit in a public hospital in southern Brazil. This retrospective cohort study used real-world data from one reference hospital, focusing on the intervention (stroke unit) and comparing it to the general ward (control). We analyzed the electronic medical records of 674 patients admitted from 2009 to 2012 in the general ward and 766 patients from 2013 to 2018 in the stroke unit. Admission to the stroke unit was associated with a 43% reduction in the likelihood of prolonged hospitalization. However, there was no significant difference in the risk of in-hospital mortality between the groups (Hazard ratio = 0.90; Interquartile range = 0.58 to 1.39). The incidence of death at three, six and twelve months post-discharge did not differ between the groups. Our study results indicate significant improvements in care processes for SU patients, including shorter LOS and better adherence to treatment protocols. However, our observations revealed no significant difference in mortality rates, either during hospitalization or after discharge, between the SU and GW groups. While SU implementation enhances efficiency in stroke care, further research is needed to explore long-term outcomes and optimize management strategies.
Identification of AURKA as a Biomarker Associated with Cuproptosis and Ferroptosis in HNSCC
Authors: Jia Xiao , Tian Jiao , Fu Yueyue , Wang Yiqi , Yang Yang , Zhang Mengzhou , Yang Cheng , Liu Yijin
Published: April 2024

Cuproptosis and ferroptosis represent copper- and iron-dependent forms of cell death, respectively, and both are known to play pivotal roles in head and neck squamous cell carcinoma (HNSCC). However, few studies have explored the prognostic signatures related to cuproptosis and ferroptosis in HNSCC. Our objective was to construct a prognostic model based on genes associated with cuproptosis and ferroptosis. We randomly assigned 502 HSNCC samples from The Cancer Genome Atlas (TCGA) into training and testing sets. Pearson correlation analysis was utilized to identify cuproptosis-associated ferroptosis genes in the training set. Cox proportional hazards (COX) regression and least absolute shrinkage operator (LASSO) were employed to construct the prognostic model. The performance of the prognostic model was internally validated using single-factor COX regression, multifactor COX regression, Kaplan–Meier analysis, principal component analysis (PCA), and receiver operating curve (ROC) analysis. Additionally, we obtained 97 samples from the Gene Expression Omnibus (GEO) database for external validation. The constructed model, based on 12 cuproptosis-associated ferroptosis genes, proved to be an independent predictor of HNSCC prognosis. Among these genes, the increased expression of aurora kinase A (AURKA) has been implicated in various cancers. To further investigate, we employed small interfering RNAs (siRNAs) to knock down AURKA expression and conducted functional experiments. The results demonstrated that AURKA knockdown significantly inhibited the proliferation and migration of HNSCC cells (Cal27 and CNE2). Therefore, AURKA may serve as a potential biomarker in HNSCC.
Effects of Resveratrol on In Vivo Ovarian Cancer Cells Implanted on the Chorioallantoic Membrane (CAM) of a Chicken Embryo Model
Authors: Chitcholtan Kenny , Singh Melanie , Tino Alex , Garrill Ashley , Sykes Peter
Published: April 2024

Ovarian cancer poses a significant threat to patients in its advanced stages, often with limited treatment options available. In such cases, palliative management becomes the primary approach to maintaining a reasonable quality of life. Therefore, the administration of any medication that can benefit patients without a curative option holds potential. Resveratrol, a natural compound known for its in vitro anticancer activities, has generated contrasting results in vivo and human studies. In this study, we aimed to assess the anticancer effects of resveratrol on ovarian cancer cells grown on the chorioallantoic membrane (CAM) of chicken embryos. Two ovarian cancer cell lines, OVCAR-8 and SKOV-3, were cultured in collagen scaffolds for four days before being implanted on the CAM of chicken embryos on day 7. Different doses of resveratrol were applied to the CAM every two days for six days. Subsequently, CAM tissues were excised, fixed, and subjected to histological analysis. Some CAM tumours were extracted to analyse proteins through Western blotting. Our findings indicate that specific doses of resveratrol significantly reduce angiogenic activities, pNF-κB levels, and SLUG protein levels by using immunohistochemistry. These results suggest that resveratrol may have the potential to impact the behaviour of ovarian cancer CAM tumours, thereby warranting further consideration as a complementary treatment option for women with incurable ovarian cancer.
The Inhibition of NS2B/NS3 Protease: A New Therapeutic Opportunity to Treat Dengue and Zika Virus Infection
Authors: Starvaggi Josè , Previti Santo , Zappalà Maria , Ettari Roberta
Published: April 2024

In the global pandemic scenario, dengue and zika viruses (DENV and ZIKV, respectively), both mosquito-borne members of the flaviviridae family, represent a serious health problem, and considering the absence of specific antiviral drugs and available vaccines, there is a dire need to identify new targets to treat these types of viral infections. Within this drug discovery process, the protease NS2B/NS3 is considered the primary target for the development of novel anti-flavivirus drugs. The NS2B/NS3 is a serine protease that has a dual function both in the viral replication process and in the elusion of the innate immunity. To date, two main classes of NS2B/NS3 of DENV and ZIKV protease inhibitors have been discovered: those that bind to the orthosteric site and those that act at the allosteric site. Therefore, this perspective article aims to discuss the main features of the use of the most potent NS2B/NS3 inhibitors and their impact at the social level.
Molecular Mechanisms Responsible for the Therapeutic Potential of Mesenchymal Stem Cell-Derived Exosomes in the Treatment of Lung Fibrosis
Authors: Harrell Carl Randall , Djonov Valentin , Volarevic Ana , Arsenijevic Aleksandar , Volarevic Vladislav
Published: April 2024

Mesenchymal stem cell-derived exosomes (MSC-Exos) are nano-sized extracellular vesicles which contain various MSC-sourced anti-fibrotic, immunoregulatory and angio-modulatory proteins (growth factors, immunoregulatory cytokines, chemokines), lipids, and nucleic acids (messenger RNA and microRNAs). Due to their lipid envelope, MSC-Exos easily by-pass all barriers in the body and deliver their cargo directly in target cells, modulating their viability, proliferation, phenotype and function. The results obtained in recently published experimental studies demonstrated beneficial effects of MSC-Exos in the treatment of lung fibrosis. MSC-Exos reduced activation of fibroblasts and prevented their differentiation in myofibroblasts. By delivering MSC-sourced immunoregulatory factors in lung-infiltrated monocytes and T cells, MSC-Exos modulate their function, alleviating on-going inflammation and fibrosis. MSC-Exos may also serve as vehicles for the target delivery of anti-fibrotic and immunomodulatory agents, enabling enhanced attenuation of lung fibrosis. Although numerous pre-clinical studies have demonstrated the therapeutic potential of MSC-Exos in the treatment of pulmonary fibrosis, there are several challenges that currently hinder their clinical implementation. Therefore, in this review article, we summarized current knowledge and we discussed future perspectives regarding molecular and cellular mechanisms which were responsible for the anti-fibrotic, anti-inflammatory and immunoregulatory properties of MSC-Exos, paving the way for their clinical use in the treatment of lung fibrosis.
The Development of Toxoplasma gondii Recombinant Trivalent Chimeric Proteins as an Alternative to Toxoplasma Lysate Antigen (TLA) in Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Immunoglobulin G (IgG) in Small Ruminants
Authors: Ferra Bartłomiej Tomasz , Chyb Maciej , Sołowińska Karolina , Holec-Gąsior Lucyna , Skwarecka Marta , Baranowicz Karolina , Gatkowska Justyna
Published: April 2024

This study presents an evaluation of seventeen newly produced recombinant trivalent chimeric proteins (containing the same immunodominant fragment of SAG1 and SAG2 of Toxoplasma gondii antigens, and an additional immunodominant fragment of one of the parasite antigens, such as AMA1, GRA1, GRA2, GRA5, GRA6, GRA7, GRA9, LDH2, MAG1, MIC1, MIC3, P35, and ROP1) as a potential alternative to the whole-cell tachyzoite lysate (TLA) used in the detection of infection in small ruminants. These recombinant proteins, obtained by genetic engineering and molecular biology methods, were tested for their reactivity with specific anti-Toxoplasma IgG antibodies contained in serum samples of small ruminants (192 samples of sheep serum and 95 samples of goat serum) using an enzyme-linked immunosorbent assay (ELISA). The reactivity of six recombinant trivalent chimeric proteins (SAG1-SAG2-GRA5, SAG1-SAG2-GRA9, SAG1-SAG2-MIC1, SAG1-SAG2-MIC3, SAG1-SAG2-P35, and SAG1-SAG2-ROP1) with IgG antibodies generated during T. gondii invasion was comparable to the sensitivity of TLA-based IgG ELISA (100%). The obtained results show a strong correlation with the results obtained for TLA. This suggests that these protein preparations may be a potential alternative to TLA used in commercial tests and could be used to develop a cheaper test for the detection of parasite infection in small ruminants.
Alteration of Gut Microbiota Composition in the Progression of Liver Damage in Patients with Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)
Authors: Zazueta Alejandra , Valenzuela-Pérez Lucía , Ortiz-López Nicolás , Pinto-León Araceli , Torres Verónica , Guiñez Danette , Aliaga Nicolás , Merino Pablo , Sandoval Alexandra , Covarrubias Natalia , Pérez de Arce Edith , Cattaneo Máximo , Urzúa Alvaro , Roblero Juan Pablo , Poniachik Jaime , Gotteland Martín , Magne Fabien , Beltrán Caroll Jenny
Published: April 2024

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a complex disorder whose prevalence is rapidly growing in South America. The disturbances in the microbiota–gut–liver axis impact the liver damaging processes toward fibrosis. Gut microbiota status is shaped by dietary and lifestyle factors, depending on geographic location. We aimed to identify microbial signatures in a group of Chilean MASLD patients. Forty subjects were recruited, including healthy controls (HCs), overweight/obese subjects (Ow/Ob), patients with MASLD without fibrosis (MASLD/F−), and MASLD with fibrosis (MASLD/F+). Both MASLD and fibrosis were detected through elastography and/or biopsy, and fecal microbiota were analyzed through deep sequencing. Despite no differences in α- and β-diversity among all groups, a higher abundance of Bilophila and a lower presence of Defluviitaleaceae, Lachnospiraceae ND3007, and Coprobacter was found in MASLD/F− and MASLD/F+, compared to HC. Ruminococcaceae UCG-013 and Sellimonas were more abundant in MASLD/F+ than in Ow/Ob; both significantly differed between MASLD/F− and MASLD/F+, compared to HC. Significant positive correlations were observed between liver stiffness and Bifidobacterium, Prevotella, Sarcina, and Acidaminococcus abundance. Our results show that MASLD is associated with changes in bacterial taxa that are known to be involved in bile acid metabolism and SCFA production, with some of them being more specifically linked to fibrosis.
QTL Analysis for Rice Quality-Related Traits and Fine Mapping of qWCR3
Authors: Liu Jun , Zhang Hao , Wang Yingying , Liu Enyu , Shi Huan , Gao Guanjun , Zhang Qinglu , Lou Guangming , Jiang Gonghao , He Yuqing
Published: April 2024

The quality of rice, evaluated using multiple quality-related traits, is the main determinant of its market competitiveness. In this study, two japonica rice varieties with significant differences in quality-related traits were used as parents to construct two populations, BC3F2 and BC3F2:3, with Kongyu131 (KY131) as the recurrent parent. A genetic linkage map was constructed using the BC3F2 population based on 151 pairs of SSR/InDel polymorphic markers selected between the parents. Grain-shape-related traits (grain length GL, grain width GW, and length-to-width ratio LWR), chalkiness-related traits (white-core rate WCR, white-belly rate WBR, white-back rate BR, and chalkiness rate CR), and amylose content (AC) were investigated in the two populations in 2017 and 2018. Except for BR and CR, the traits showed similar characteristics with a normal distribution in both populations. Genetic linkage analysis was conducted for these quality-related traits, and a total of 37 QTLs were detected in the two populations. Further validation was performed on the newly identified QTLs with larger effects, and three grain shape QTLs and four chalkiness QTLs were successfully validated in different environments. One repeatedly validated QTL, qWCR3, was selected for fine mapping and was successfully narrowed down to a 100 kb region in which only two genes, LOC_0s03g45210 and LOC_0s03g45320, exhibited sequence variations between the parents. Furthermore, the variation of LOC_Os03g45210 leads to a frameshift mutation and premature protein termination. The results of this study provide a theoretical basis for positional cloning of the qWCR3 gene, thus offering new genetic resources for rice quality improvement.
A Large-Crack Image-Stitching Method with Cracks as the Regions of Interest
Authors: Kao Szu-Pyng , Lin Jhih-Sian , Wang Feng-Liang , Hung Pen-Shan
Published: April 2024

While crack detection is crucial for maintaining concrete structures, existing methods often overlook the analysis of large cracks that span multiple images. Such analyses typically rely on image stitching to create a complete image of a crack. Current stitching methods are not only computationally demanding but also require manual adjustments; thus, a fast and reliable solution is still lacking. To address these challenges, we introduce a stitching method that leverages the advantages of crack image-segmentation models. This method first utilizes the Mask R-CNN model for the identification of crack regions as regions of interest (ROIs) within images. These regions are then used to calculate keypoints of the scale-invariant feature transform (SIFT), and descriptors for these keypoints are computed with the original images for image matching and stitching. Compared with traditional methods, our approach significantly reduces the computational time; by 98.6% in comparison to the Brute Force (BF) matcher, and by 58.7% with respect to the Fast Library for Approximate Nearest Neighbors (FLANN) matcher. Our stitching results on images with different degrees of overlap or changes in shooting posture show superior structural similarity index (SSIM) values, demonstrating excellent detail-matching performance. Moreover, the ability to measure complete crack images is indicated by the relative error of 7%, which is significantly better than that of traditional methods.
Methods for Indirect Treatment Comparison: Results from a Systematic Literature Review
Authors: Macabeo Bérengère , Quenéchdu Arthur , Aballéa Samuel , François Clément , Boyer Laurent , Laramée Philippe
Published: April 2024

Introduction: Health technology assessment (HTA) agencies express a clear preference for randomized controlled trials when assessing the comparative efficacy of two or more treatments. However, an indirect treatment comparison (ITC) is often necessary where a direct comparison is unavailable or, in some cases, not possible. Numerous ITC techniques are described in the literature. A systematic literature review (SLR) was conducted to identify all the relevant literature on existing ITC techniques, provide a comprehensive description of each technique and evaluate their strengths and limitations from an HTA perspective in order to develop guidance on the most appropriate method to use in different scenarios. Methods: Electronic database searches of Embase and PubMed, as well as grey literature searches, were conducted on 15 November 2021. Eligible articles were peer-reviewed papers that specifically described the methods used for different ITC techniques and were written in English. The review was performed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Results: A total of 73 articles were included in the SLR, reporting on seven different ITC techniques. All reported techniques were forms of adjusted ITC. Network meta-analysis (NMA) was the most frequently described technique (in 79.5% of the included articles), followed by matching-adjusted indirect comparison (MAIC) (30.1%), network meta-regression (24.7%), the Bucher method (23.3%), simulated treatment comparison (STC) (21.9%), propensity score matching (4.1%) and inverse probability of treatment weighting (4.1%). The appropriate choice of ITC technique is critical and should be based on the feasibility of a connected network, the evidence of heterogeneity between and within studies, the overall number of relevant studies and the availability of individual patient-level data (IPD). MAIC and STC were found to be common techniques in the case of single-arm studies, which are increasingly being conducted in oncology and rare diseases, whilst the Bucher method and NMA provide suitable options where no IPD is available. Conclusion: ITCs can provide alternative evidence where direct comparative evidence may be missing. ITCs are currently considered by HTA agencies on a case-by-case basis; however, their acceptability remains low. Clearer international consensus and guidance on the methods to use for different ITC techniques is needed to improve the quality of ITCs submitted to HTA agencies. ITC techniques continue to evolve quickly, and more efficient techniques may become available in the future.
Sponge Communities of Submarine Caves and Tunnels on the Fernando de Noronha Archipelago, Northeast Brazil
Authors: Muricy Guilherme , Lage Anaíra , Sandes Joana , Klautau Michelle , Pinheiro Ulisses , Laport Marinella Silva , de Oliveira Bruno Francesco Rodrigues , Pequeno Carolline Braga , Lopes Matheus Vieira
Published: April 2024

Submarine caves are important biodiversity reservoirs, but there is little information about the biota of marine caves in the Southwestern Atlantic. Here, we describe three submarine cavities and their sponge communities on the Fernando de Noronha Archipelago, Northeast Brazil. The underwater cavities were explored and collections were made through scuba diving from 5 to 18 m depths. Sapata Cave has a wide semi-dark zone near the entrance, a narrow transition zone, and a dark chimney, which is closed at the top. Ilha do Meio Cave is narrower and shallower than Sapata Cave, but has a long passage that leads to two completely dark rooms. Pedras Secas Tunnel has only a semi-dark zone with high water movement. The sponge communities in the semi-dark zones of the three cavities are rich and dominated by the classes Demospongiae and Homoscleromorpha, but Calcarea are also common. The transition zones of both caves are dominated by a desma-bearing sponge, thinly encrusting spirastrellids, and small Homoscleromopha and Calcarea. The dark zone in Ilha do Meio Cave is almost azoic, with only three species. This study has increased the number of sponge species known in submarine cavities on Fernando de Noronha from 29 to 69, highlighting the great richness of the sponge communities in these cryptic environments.
The Diversification Benefits of Foreign Real Estate: Evidence from 40 Years of Data
Authors: Conover C. Mitchell , Farizo Joseph D. , Friday H. Swint , North David S.
Published: April 2024

We investigate the potential of global real estate to improve the long-term performance of a US equity portfolio, utilizing a recent dataset of 40 years’ worth of US stocks, US real estate, 13 foreign stock markets, and 13 foreign real estate markets across diverse regions. Despite a modest performance in terms of risk and return, foreign real estate has consistently lower correlations with US stocks compared to foreign equities. Rolling correlation analysis indicates that foreign real estate markets remain relatively segmented compared to foreign equity, despite increasing financial market correlations over time. Efficient frontier analysis demonstrates that portfolios including foreign real estate consistently outperform those limited to US stocks and US real estate or those including foreign stocks, indicating the importance of foreign real estate in optimizing portfolio performance. Subperiod analysis reveals that foreign real estate retains its diversification benefits even in the latter, more integrated period. Our results are robust when using Conditional Value-at-Risk as a measure of risk. Overall, our findings highlight the persistent diversification benefits and superior risk-adjusted returns from incorporating foreign real estate into US equity portfolios.
Characterization of CuAg Alloys with Low Ag Concentrations
Authors: Mosesso Lorenzo , Macis Salvatore , D’Arco Annalisa , Marcelli Augusto , Notargiacomo Andrea , Pea Marialilia , Spataro Bruno , Stagno Vincenzo , Lupi Stefano
Published: April 2024

Copper-based alloys designed to combine high electronic and thermal conductivities with high mechanical strength find a wide range of applications in different fields. Among the principal representatives, strongly diluted CuAg alloys are of particular interest as innovative materials for the realization of accelerating structures when the use of high-gradient fields requires increasingly high mechanical and thermal performances to overcome the limitations induced by breakdown phenomena. This work reports the production and optical characterization of CuAg crystals at low Ag concentrations, from 0.028% wt to 0.1% wt, which guarantee solid solution hardening while preserving the exceptional conductivity of Cu. By means of Fourier Transform Infrared (FTIR) micro-spectroscopy experiments, the low-energy electrodynamics of the alloys are compared with that of pure Cu, highlighting the complete indistinguishability in terms of electronic transport for such low concentrations. The optical data are further supported by Raman micro-spectroscopy and SEM microscopy analyses, allowing the demonstration of the full homogeneity and complete solubility of solid Ag in copper at those concentrations. Together with the solid solution hardening deriving from the alloying process, these results support the advantage of strongly diluted CuAg alloys over conventional materials for their application in particle accelerators.
Valorization of Water Treatment Sludge for Applications in the Construction Industry: A Review
Authors: Mattoso Ana Paula , Cunha Sandra , Aguiar José , Duarte António , Lemos Helena
Published: April 2024

To address the growing global water demand, it is imperative to implement advanced treatment systems and sustainable alternatives for managing the large amount of waste generated during the water purification process, known as water treatment sludge (WTS). Worldwide, researchers and companies are exploring alternatives and methods for the valorization of WTS as a raw material in other processes. It is urgent that all productive sectors, which contribute significantly to greenhouse gas emissions, adopt this management principle to ensure more sustainable production, contributing to the global goal of climate neutrality. Notably, in civil construction, incorporating WTS as a supplementary cementitious material (SCM) shows great promise, considering that the industrial waste currently used for this purpose is increasingly restricted. The use of WTS as a raw material in the cement industry not only contributes to the reduction of the carbon footprint, but also reduces the high waste load still disposed of in landfills. The emerging applications for WTP sludge are reviewed, with emphasis on its valorization in the civil construction as an SCM. The main characteristics of this waste and their impacts on the environment are also addressed.
Extrinsic-Riveting Friction Stir Lap Welding of Al/Steel Dissimilar Materials
Authors: Meng Xiangchen , Gao Jiaze , Xie Yuming , Huang Tifang , Dong Jihong , Ma Xiaotian , Wang Naijie , Huang Yongxian
Published: April 2024

To obtain high-quality joints of Al/steel dissimilar materials, a new extrinsic-riveting friction stir lap welding (ERFSLW) method was proposed combining the synthesis advantages of mechanical riveting and metallurgical bonding. SiC-reinforced Al matrix composite bars were placed in the prefabricated holes in Al sheets and steel sheets, arranged in a zigzag array. The bars were stirred and mixed with Al sheets under severe plastic deformation (SPD), forming composite rivets to strengthen the mechanical joining. SiC particles were uniformly dispersed in the lower part of the welding nugget zone (WNZ). The smooth transition between the SiC mixed zone and extrinsic-riveting zone (ERZ) ensured the metallurgical bonding. The maximum tensile shear load of the joints reached 7.8 kN and the maximum load of the weld per unit length was 497 N/mm. The fracture occurred at the interface between the rivets and steel sheets rather than the conventional Al/steel joining interface. Moreover, ERFSLW can prolong the service life of joints due to three fracture stages. This method can be further extended to the welding of other dissimilar materials that conform to the model of “soft/hard”.
High-Throughput Screening Method Using Escherichia coli Keio Mutants for Assessing Primary Damage Mechanism of Antimicrobials
Authors: Martínez-Álvarez José A. , Vicente-Gómez Marcos , García-Contreras Rodolfo , Wood Thomas K. , Ramírez Montiel Fátima Berenice , Vargas-Maya Naurú Idalia , España-Sánchez Beatriz Liliana , Rangel-Serrano Ángeles , Padilla-Vaca Felipe , Franco Bernardo
Published: April 2024

The Escherichia coli Keio mutant collection has been a tool for assessing the role of specific genes and determining their role in E. coli physiology and uncovering novel functions. In this work, specific mutants in the DNA repair pathways and oxidative stress response were evaluated to identify the primary targets of silver nanoparticles (NPs) and their mechanism of action. The results presented in this work suggest that NPs mainly target DNA via double-strand breaks and base modifications since the recA, uvrC, mutL, and nfo mutants rendered the most susceptible phenotype, rather than involving the oxidative stress response. Concomitantly, during the establishment of the control conditions for each mutant, the katG and sodA mutants showed a hypersensitive phenotype to mitomycin C, an alkylating agent. Thus, we propose that KatG catalase plays a key role as a cellular chaperone, as reported previously for the filamentous fungus Neurospora crassa, a large subunit catalase. The Keio collection mutants may also be a key tool for assessing the resistance mechanism to metallic NPs by using their potential to identify novel pathways involved in the resistance to NPs.
Isolation and Identification of Chicken-Derived Lactic Acid Bacteria: In Vitro Probiotic Properties and Antagonistic Effects against Salmonella pullorum, Staphylococcus aureus, and Escherichia coli
Authors: Tian Congcong , Wang Lei , Liu Mengjian , Liu Jiancheng , Qiu Mingxin , Chen Yong
Published: April 2024

The development of probiotics capable of quickly colonizing the intestines of animals is important in promoting the healthy growth of livestock. The aim of this study was to screen lactic acid bacteria (LAB) from the intestinal microbiota of chickens with potential applications, and to evaluate their probiotic properties and antagonistic abilities against Salmonella pullorum, Staphylococcus aureus, and Escherichia coli. The results showed that a total of 79 strains with the characteristics of LAB were isolated from the chicken cecum microbiota, of which 7 strains exhibited strong inhibitory activity against S. pullorum, S. aureus, and E. coli. Performing 16s rDNA sequencing revealed that these seven strains were Lactiplantibacillus pentosus (n = 1), Lactiplantibacillus plantarum (n = 3), Lactiplantibacillus paraplantarum (n = 1), Lactiplantibacillus argentoratensis (n = 1), and Lactiplantibacillus fabifermentans (n = 1). Among them, L. pentosus R26 and L. plantarum R32 exhibited superior antibacterial activity. These two strains demonstrated high lactic acid production ability, with survival rates of 86.29% and 87.99% after 3 h of treatment at pH 1.5, 86.66% and 85.52% after 3 h of treatment with 0.5% bile salts, 90.03% and 88.16% after 2 h of treatment with simulated gastric fluid, and 98.92% and 98.22% after 2 h of treatment with simulated intestinal fluid, respectively. Co-cultivation with L. pentosus R26 for 24 h resulted in 50% of the pathogens being antagonized, while almost complete inhibition was observed following 72 h of co-cultivation. In conclusion, L. pentosus R26 and L. plantarum R32 exhibited high antibacterial activity and acid production capability, while also demonstrating satisfactory tolerance to low pH values and high concentrations of bile salts and digestive fluid. The probiotic characteristics and stress resistance of L. pentosus R26 were slightly superior to those of L. plantarum R32, indicating its potential for development as a probiotic.
Whole Genome Analysis of Streptomyces spp. Strains Isolated from the Rhizosphere of Vitis vinifera L. Reveals Their Role in Nitrogen and Phosphorus Metabolism
Authors: Montes-Montes Gustavo , González-Escobedo Román , Muñoz-Castellanos Laila N. , Avila-Quezada Graciela D. , Ramírez-Sánchez Obed , Borrego-Loya Alejandra , Ortiz-Aguirre Ismael , Muñoz-Ramírez Zilia Y.
Published: April 2024

The rhizospheric microorganisms of agricultural crops play a crucial role in plant growth and nutrient cycling. In this study, we isolated two Streptomyces strains, Streptomyces sp. LM32 and Streptomyces sp. LM65, from the rhizosphere of Vitis vinifera L. We then conducted genomic analysis by assembling, annotating, and inferring phylogenomic information from the whole genome sequences. Streptomyces sp. strain LM32 had a genome size of 8.1 Mb and a GC content of 72.14%, while Streptomyces sp. strain LM65 had a genome size of 7.3 Mb and a GC content of 71%. Through ANI results, as well as phylogenomic, pan-, and core-genome analysis, we found that strain LM32 was closely related to the species S. coelicoflavus, while strain LM65 was closely related to the species S. achromogenes subsp. achromogenes. We annotated the functional categories of genes encoded in both strains, which revealed genes involved in nitrogen and phosphorus metabolism. This suggests that these strains have the potential to enhance nutrient availability in the soil, promoting agricultural sustainability. Additionally, we identified gene clusters associated with nitrate and nitrite ammonification, nitrosative stress, allantoin utilization, ammonia assimilation, denitrifying reductase gene clusters, high-affinity phosphate transporter and control of PHO regulon, polyphosphate, and phosphate metabolism. These findings highlight the ecological roles of these strains in sustainable agriculture, particularly in grapevine and other agricultural crop systems.