bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatiotemporal Dynamics of Wetland in Dongting Lake Based on Multi-Source Satellite Observation Data during Last Two Decades
Authors: Xing Liwei , Chi Liang , Han Shuqing , Wu Jianzhai , Zhang Jing , Jiao Cuicui , Zhou Xiangyang
Published: October 2022

Monitoring the dynamics of wetland resources has practical value for wetland protection, restoration and sustainable utilization. Dongting Lake wetland reserves are well known for both their intra-annual and inter-annual dynamic changes due to the effects of natural or human factors. However, most wetland monitoring research has failed to consider the seasonal wetlands, which is the most fragile wetland type, requiring more attention. In this study, we used multi-source time series remote sensing data to monitor three Dongting Lake wetland reserves between 2000 and 2020, and the seasonal wetlands were separated from permanent wetlands. Multispectral and indices time series were generated at 30 m resolution using a two-month composition strategy; the optimal features were then selected using the extension of the Jeffries–Matusita distance (JBh) and random forest (RF) importance score; yearly wetland maps were identified using the optimal features and the RF classifier. Results showed that (1) the yearly wetland maps had good accuracy, and the overall accuracy and kappa coefficients of all wetland maps from 2000 to 2020 were above 89.6% and 0.86, respectively. Optimal features selected by JBh can improve both computational efficiency and classification accuracy. (2) The acreage of seasonal wetlands varies greatly among multiple years due to inter-annual differences in precipitation and evaporation. (3) Although the total wetland area of the three Dongting Lake wetland reserves remained relatively stable between 2000 and 2020, the acreage of the natural wetland types still decreased by 197.0 km2, and the change from natural wetland to human-made wetland (paddy field) contributed the most to this decrease. From the perspective of the ecological community, the human-made wetland has lower ecological function value than natural wetlands, so the balance between economic development and ecological protection in the three Dongting Lake wetland reserves requires further evaluation. The outcomes of this study could improve the understanding of the trends and driving mechanisms of wetland dynamics, which has important scientific significance and application value for the protection and restoration of Dongting Lake wetland reserves.
Effects of Vaping Prevention Messages on Electronic Vapor Product Beliefs, Perceived Harms, and Behavioral Intentions among Young Adults: A Randomized Controlled Trial
Authors: Villanti Andrea C. , Wackowski Olivia A. , LePine S. Elisha , West Julia C. , Stevens Elise M. , Unger Jennifer B. , Mays Darren
Published: October 2022

Youth have been the focus of electronic vapor product (EVP) prevention efforts though young adults had similar increases in current EVP use from 2015–2019. This study tested messages to reduce EVP use in young adults. Eight messages on vaping related harms and addictiveness combined with themes on social use and flavors were selected for inclusion in an online randomized controlled trial. Vermont young adults aged 18–24 (n = 569) were randomized to view the eight vaping prevention messages (n = 295) or eight messages on sun safety (n = 274). After completing baseline measures, participants viewed study messages and completed measures on message perceptions and perceived message effectiveness (PME), EVP-related beliefs, and EVP-related harm perceptions. Participants completed EVP-related beliefs and harm perception measures again at 1-month follow-up, as well as measures on tobacco and EVP-related behavioral intentions and behavior (ever and past 30-day use). Intervention participants reported positive impacts on vaping-related message responses. However, findings suggested no effect of vaping prevention messages on EVP-related beliefs, harm perceptions, or behaviors in the full sample. Exploratory analyses in the intervention condition showed that greater PME was associated with lower odds to intent to try cigarettes in the next year at follow-up.
Outcomes of Transvenous Extraction of Leads Older Than 20 and 30 Years—A Large Cohort Study
Authors: Kutarski Andrzej , Głowniak Andrzej , Kosior Jarosław , Jacheć Wojciech , Nowosielecka Dorota , Czajkowski Marek , Polewczyk Anna
Published: October 2022

Background: There is limited knowledge on outcome of transvenous lead extraction (TLE) of leads being 20 and 30 years old. Methods: Retrospective single center large database analysis containing 3673 TLE procedures performed from 2006 to 2020 was analysed. We aimed to compare procedure complexity and the incidence of the TLE major complications (MC) in groups where extracted leads were under 10 years, 10–20 years, 20–30 years (old) and over 30 years (very old). Results: Rate of removal of old and very old leads almost doubles with successive five-year periods (3–6-10%). In patients with old and very old leads there is an accumulation of risk factors for major complications of TLE (young age, female, multiple and/or abandoned leads, multiple previous procedures). The removal of old and very old leads was more labour-consuming, more difficult, and much more often required second-line (advanced) tools and complex techniques. Incidence of all MC grew parallel to age of removed leads from 0.6 to 18.2%; haemopericardium—from 0.3 to 12.1%, severe tricuspid valve damage—from 0.2 to 2.1%, need for rescue cardiac surgery—from 0.4 to 9.1%. Notably, there was no procedure-related death when old or very old lead was extracted. The percentages of clinical and procedural success decreased with increasing age of the removed leads from 99.2 and 97.8% to 90.9 and 81.8%. The risk of MC during extraction of leads aged 10–20 years increases 6.7 times, aged 20–30 years—14.3 times (amounting to 8.4%), and aged 30 and more years—20.4 times, amounting to 18.2%. Removal of ventricular leads is associated with a greater complexity of the procedure but not with more frequent MC. Removal of the atrial leads is associated with a higher incidence of MC, especially haemopericardium, regardless of the age of the leads, although the tendency becomes less pronounced with the oldest leads. Conclusions: 1. Extraction of old and very old leads is a rising challenge, since the rate of removal of leads aged 20-and-more years almost doubles with successive five-year periods. 2. Procedure difficulty, complexity and the risk of major complications increases along with the age of extracted lead. TLE is more time-consuming, difficult and much more often requires advanced tools and complex techniques. 3. TLE of old (≥20 years) or very old (≥30 years) leads can be performed with satisfactory success rate and safety profile when conducted at high-volume centre by an experienced operator under optimal safety conditions.
Designing Innovative Assistive Technology Devices for Tourism
Authors: Zabłocki Marek , Branowski Bogdan , Kurczewski Przemysław , Gabryelski Jarosław , Sydor Maciej
Published: October 2022

Active tourism improves human health and well-being regardless of age or disabilities. The paper analyses and describes current issues concerning the tourism of people with disabilities. The starting point is the currently insufficient availability of tourist offers for individuals with considerable motor dysfunctions. One of the causes for these limitations stems from deficiencies in transport means for people with disabilities. It was found that for a disabled passenger using public transport, it is crucial to consider its accessibility in the context of the entire transport system. Another cause is the limited popularity of innovative, atypical assistive equipment for people with disabilities. Those insights point out that novel assistive technologies need to be developed, as it is necessary to more effectively support the activity of people with disabilities in all areas of life, including tourism, as this enhances their social rehabilitation. This paper indicates the needs and describes and analyses examples of own original, innovative devices supporting the areas mentioned above of activity for people with disabilities. These analyses resulted in developing an algorithm to design innovative equipment, considerably expanding the tourism potential of people with motor disabilities. This design process focuses on the needs of people with disabilities and facilitates the development of novel classes of assistive technologies, thus promoting new areas of activity for all.
The Use of Infrared Thermography in the Assessment of Thermal Reaction of Patients Treated with Radiotherapy after Breast-Conserving Procedures
Authors: Plaza Dominika , Baic Agnieszka , Lange Barbara , Michalecki Łukasz , Ślosarek Krzysztof , Stanek Agata , Cholewka Armand
Published: October 2022

The aim of the study was to assess the usefulness of the infrared thermography method in the assessment of the body’s thermal reaction in patients during radiotherapy. Studies have shown how the temperature distribution changes with the dose of radiation used in each treatment week. Fifty-four patients participated in the study. The control group consisted of healthy patients, among whom the difference in mean temperatures between the breasts was checked. The study group included patients after conserving procedures qualified for radiotherapy. Measurements were taken and analyzed for each patient during each week of treatment. The target area (marked by a doctor) and the 30 Gy, 20 Gy and 10 Gy isodoses (generated from the treatment plan) were transferred on thermograms and then analyzed. This way of defining the observed areas is the most accurate and individually adjusted to each treated patient. The analysis showed an increase in temperature in the tested body surface areas, whereas the highest average temperature value was achieved during the third week of treatment. The observations may be used to evaluate the skin reaction from radiation; however, they require further studies and new quantitative parameters.
The Removal of Cr(VI) from Aqueous Solutions with Corn Stalk Biochar
Authors: Yang Wenling , Lei Gao , Quan Shujing , Zhang Longfei , Wang Baitao , Hu Hong , Li Liangliang , Ma Huan , Yin Chaohui , Feng Fei , Jing Yanyan
Published: October 2022

The discharge of wastewater containing hexavalent chromium (Cr(VI)) into the environment is very harmful to living things. Therefore, before effluent that contains Cr(VI) can be discharged into the environment, this toxin should be removed from the contaminated water. In this study, corn stalk biochar was investigated to evaluate the Cr(VI) removal efficiency from an aqueous solution. The effects of pH (2–10), biochar concentration (0.5 to 10 g/L), Cr(VI) concentration (10–500 mg/L), and contact time (10–1440 min) were studied. The actual experimental value of the Cr(VI) removal efficiency was 28.67%, largely consistent with the predicted model value of 29.31%, under the optimal conditions of a Cr(VI) concentration of 60 g/L, pH 4, contact time of 270 min, and a biochar concentration of 4.5 g/L. A significant interaction between the Cr(VI) concentration and pH was observed, along with significance in the interaction between Cr(VI) concentration and biochar concentration, which had a greater impact on the removal of Cr(VI). Biosorption onto corn stalk biochar is an affordable and economical adsorption process to treat wastewater contaminated with Cr(VI). The aim of this study is to provide data to serve as a basis for future studies on the use of raw agricultural waste to remove Cr(VI).
Chasing the Youth Dividend in Nigeria, Malawi and South Africa: What Is the Role of Poverty in Determining the Health and Health Seeking Behaviour of Young Women?
Authors: Mkwananzi Sibusiso , Baruwa Ololade Julius
Published: October 2022

Africa’s new source of hope lies in harnessing Demographic Dividend, which may create a window of economic opportunity as fertility levels decrease if the correct policies and programmes are put in place. It has been shown that the health status of young people should be optimal for the realisation of the demographic dividend. This study examined the association between poverty and the health status and health seeking behaviour of young women (15–25), using the Demographic Health surveys of Nigeria 2013, Malawi 2015–2016, and South Africa 2015–2016. Interest variables were household and community-levels of poverty, while the outcomes were pregnancy and HIV testing and health services seeking. Results showed that in Malawi only household poverty was associated with a higher likelihood of pregnancy, while in South Africa household- and community-levels of poverty were associated with a higher likelihood of pregnancy. In Nigeria, household- and community-levels of poverty were not significantly linked to pregnancy but were associated with a lower likelihood of HIV testing and health seeking behaviour. The study shows empirical evidence of the construction of negative health outcomes in poor households and communities in Nigeria, Malawi, and South Africa. Therefore, if the demographic dividend is to be a reality in the near future, it is imperative to ensure that poverty-alleviation urgently occurs.
Effectiveness of Virtual Reality on Balance and Risk of Falls in People with Multiple Sclerosis: A Systematic Review and Meta-Analysis
Authors: Castellano-Aguilera Ana , Biviá-Roig Gemma , Cuenca-Martínez Ferran , Suso-Martí Luis , Calatayud Joaquín , Blanco-Díaz María , Casaña José
Published: October 2022

The aim of this study was to systematically review the scientific evidence related to the physiotherapy interventions in neurorehabilitation that utilize virtual reality (VR) for balance training and risk of falls in people with multiple sclerosis (MS). A search was conducted in Medline (PubMed), PEDro, and Google Scholar to identify all the relevant studies. Clinical trials assessing the effects of VR in people with MS were included. Risk of bias was evaluated using the Cochrane Risk of Bias Tool and PEDro scale. Qualitative analysis was performed according to the GRADE. In total, 16 studies (n = 663) were included. The meta-analysis showed statistically significant differences for the VR intervention in comparison with conventional treatment for balance, with a moderate clinical effect in eight studies (SMD: 0.63; 95% CI 0.34–0.92; p < 0.05). In addition, the meta-analysis showed statistically significant differences for the VR intervention in comparison with conventional treatment for risk of falls, with a small clinical effect in six studies (SMD: −0.55; 95% CI −1.07–0.04; p < 0.05). VR-based treatments are more effective than non-intervention in improving balance and fall risk in people with MS, with a very low certainty of evidence. In addition, they also show to be more effective than conventional rehabilitation, with a very low certainty of evidence.
Dietitians’ Experiences of Providing Oral Health Promotion to Clients with an Eating Disorder: A Qualitative Study
Authors: Patterson-Norrie Tiffany , Ramjan Lucie , Sousa Mariana S. , George Ajesh
Published: October 2022

(1) Background: Eating disorders (EDs) can seriously impact oral health, leading to irreversible dental damage. Dietitians play a key role in the care of people with an ED and are well-placed to promote oral health. However, there is currently little understanding of how dietitians perceive their role in this space. This study aimed to explore the perceptions and role of dietitians in providing oral health promotion to their clients in an ED clinical setting. (2) Methods: This descriptive qualitative study used semi-structured interviews to explore the perceptions of 14 registered dietitians practicing across seven states in Australia. Participants were recruited using a combination of purposive and snowball sampling. A hybrid thematic analysis approach was undertaken to identify and describe the key themes generated from the data. (3) Results: Generally, dietitians were insightful and knowledgeable of the oral health issues that clients with an ED may be experiencing. However, dietitians’ practices across education, screening, and referrals were inconsistent. Challenges such as inadequate training, unknown referral pathways, and clear guidelines were cited as significant barriers to practice. (4) Conclusions: The results reinforce the need for education and the development of targeted strategies that address challenges to oral health promotion in dietetic practice.
Time-Dependent Effects of Physical Activity on Cardiovascular Risk Factors in Adults: A Systematic Review
Authors: Liu Hengxu , Liu Shiqi , Wang Kun , Zhang Tingran , Yin Lian , Liang Jiaqi , Yang Yi , Luo Jiong
Published: October 2022

Purpose: Physical activity is an important non-drug-related method to prevent and treat cardiovascular diseases, but how exercise duration affects the cardiovascular metabolic risk factors in adults remains uncertain. This review systematically examines the time-dependent effects of physical activity on cardiovascular risk factors in adults and aims to further the understanding of the temporal therapeutics of exercise. Methods: Following the PRISMA guidelines, the PubMed, Web of Science, EMBASE, and CNKI databases were systematically searched for relevant scientific studies from January 2000 to June 2022. Results: A total of 16 studies met the inclusion criteria and were included in the systematic review. The sample size ranged from 11–275 participants who were diagnosed with obesity, hypertension, diabetes mellitus type 2 (T2DM), and Coronary Heart Disease (CAD), while the subjects in four studies did not report any metabolic or cardiovascular disease. Four studies conducted trials of acute exercise interventions, while the remaining intervention periods ranged from 12 days to 12 weeks. The exercise interventions included aerobic training, resistance training, aerobic training that was combined with resistance training, compound exercise, and high-intensity interval exercise, and the training frequency varied from 2–5 times/week. Conclusions: Overall, this review found some evidence that the cardiovascular risk factors in adults may be time-dependent in response to physical activity. However, it is limited by the small sample size for each of the outcomes and several methodological issues, leading to poor comparability between studies. A randomized controlled trial with a larger sample size is supposed to be designed for the relevant population to completely test whether synchronizing the exercise time point in the day with the individual’s circadian rhythm can amplify the benefits of the exercise for improving cardiovascular health.
Effects of Multicomponent Exercise Training on the Health of Older Women with Osteoporosis: A Systematic Review and Meta-Analysis
Authors: Linhares Diego Gama , Borba-Pinheiro Claudio Joaquim , Castro Juliana Brandão Pinto de , Santos Andressa Oliveira Barros dos , Santos Luciano Lima dos , Cordeiro Lilliany de Souza , Drigo Alexandre Janotta , Nunes Rodolfo de Alkmim Moreira , Vale Rodrigo Gomes de Souza
Published: October 2022

This study aimed to analyze the effects of multicomponent exercise training in older women with osteoporosis. We conducted a systematic review following the PRISMA guidelines and registered on PROSPERO (number CRD42022331137). We searched MEDLINE (via PubMed), Web of Science, Scopus, and CINHAL databases for randomized experimental trials that analyzed the effects of physical exercise on health-related variables in older women with osteoporosis. The risk of bias in the studies was verified using the Cochrane Collaboration tool and the Jadad scale was used to assess the methodological quality of the studies. Fourteen randomized controlled trials were included, with a total of 544 participants in the experimental group and 495 in the control group. The mean age of all participants was 68.4 years. The studies combined two to four different exercise types, including strength, aerobic, balance, flexibility, and/or functional fitness training. The practice of multicomponent training with an average of 27.2 weeks, 2.6 sessions per week, and 45 min per session showed improvements in strength, flexibility, quality of life, bone mineral density, balance, and functional fitness and reduced the risk of falls in older women with osteoporosis. Multicomponent training was shown to be effective in improving health-related variables in older women with osteoporosis.
Restoring Coal Mining-Affected Areas: The Missing Ecosystem Services
Authors: Krzemień Alicja , Álvarez Fernández Juan José , Riesgo Fernández Pedro , Fidalgo Valverde Gregorio , Garcia-Cortes Silverio
Published: October 2022

Multi-criteria decision analysis and cost-benefit analysis, either individually or in combination, have been used as the preferred tools to develop ecosystem services valuation, presenting significant discrepancies and variations between the calculated values. To counteract this problem, a new framework was developed based on a hierarchical weighting of the non-provisioning ecosystem services, using biodiversity as the reference ecosystem service since it is the easiest to apprehend. Their monetisation was made using the average price of EU carbon dioxide emission allowances during 2019 and 2020, obtaining reasonable and comparable results in line with what was expected for the study region. However, the revised EU Emissions Trading System Directive, which will apply from 2021–2030, generated a price escalation of carbon allowances, making it necessary to adjust or rethink the proposed framework. To achieve this goal, the paper proposes the introduction of new vectors or “missing ecosystem services” to counterbalance efforts to eliminate carbon dioxide emissions without necessarily removing humans from the equation: welfare and human health. As the linkages regarding ecosystem health, ecological restoration and human health are not well known, only welfare was incorporated into the framework. The results were highly satisfactory, in line with what was expected for the study region and the ones obtained before the price escalation of carbon allowances that started in 2021.
A Chlamydia psittaci Outbreak in Psittacine Birds in Sardinia, Italy
Authors: Muroni Gaia , Pinna Luigia , Serra Elisa , Chisu Valentina , Mandas Daniela , Coccollone Annamaria , Liciardi Manuel , Masala Giovanna
Published: October 2022

Chlamydia psittaci is an intracellular bacterium belonging to the Chlamydiaceae family. It is the ethiologic agent of psittacosis, an occupational zoonotic disease that mainly concerns people who work in close contact with birds that represent the main infection route for human transmission. In Italy, information about this disease is lacking. This study is the first case of avian chlamydiosis reported from a pet shop in Sardinia, Italy. Chlamydia psittaci detected in psittacine birds by molecular analysis, direct immunofluorescence test together with anatomo-pathological observed lesions, highlighted the importance of focusing the attention over this underestimated zoonosis in a “One Health” prospective.
Effect of Home-Based Training with a Daily Calendar on Preventing Frailty in Community-Dwelling Older People during the COVID-19 Pandemic
Authors: Nakamura Misa , Ohki Masataka , Mizukoshi Riku , Takeno Itsuki , Tsujita Taira , Imai Ryota , Imaoka Masakazu , Takeda Masatoshi
Published: October 2022

It has been reported that marked decreases in physical activity including social activities, deterioration in eating habits and mental health, and an increase in frailty have occurred during the COVID-19 pandemic. This study aimed to devise a method to prevent the onset and progression of frailty during the COVID-19 pandemic and to verify its effect. The subjects were 111 community-dwelling older people who answered questionnaires before and after the intervention. A calendar incorporating 31 different tasks, one for each day, was created as an intervention tool with the aim of improving motor, oral, and cognitive functions. The intervention group (n = 49) participants performed these tasks every day for 3 months. The primary outcome was the Kihon checklist (KCL) score. When the amount of change in the KCL score before and after 3 months was compared between the two groups, no difference in the total score was observed between the two groups; however, the intervention group showed significantly improved cognitive function in the KCL sub-domain. In the intervention group, the number of pre-frailty and frailty patients decreased significantly after the intervention compared to before the intervention. These results suggest that the use of the calendar created in this study during the COVID-19 pandemic could prevent decreased cognitive function in the KCL sub-domain and could help prevent the onset and progression of pre-frailty and frailty.
Inappropriate Corporate Strategies: Latin American Companies That Increase Their Value by Short-Term Liabilities
Authors: Feregrino Jorge , Espinosa-Cristia Juan Felipe , Lay Nelson , Leyton Luis
Published: October 2022

This study seeks to understand the financing strategy used by companies listed on the Mexican Stock Exchange (BVM), the São Paulo Stock Exchange (VVSP), and the Santiago Stock Exchange (BCS). To this end, the data observed in the Economática database for a sample of 29 companies were considered. Then, through a long panel data model, the study concludes that in the organizations reviewed, there is a degree of association between the variables “short-term liabilities” and “share price”, as the former increases by 1%, and the value of the shares increases by 0.09% in the subsequent period. This confirms a procyclical financial leverage.
Machine Recognition of Map Point Symbols Based on YOLOv3 and Automatic Configuration Associated with POI
Authors: Zhang Huili , Zhou Xiaowen , Li Huan , Zhu Ge , Li Hongwei
Published: October 2022

This study is oriented towards machine autonomous mapping and the need to improve the efficiency of map point symbol recognition and configuration. Therefore, an intelligent recognition method for point symbols was developed using the You Only Look Once Version 3 (YOLOv3) algorithm along with the Convolutional Block Attention Module (CBAM). Then, the recognition results of point symbols were associated with the point of interest (POI) to achieve automatic configuration. To quantitatively analyze the recognition effectiveness of this study algorithm and the comparison algorithm for map point symbols, the recall, precision and mean average precision (mAP) were employed as evaluation metrics. The experimental results indicate that the recognition efficiency of point symbols is enhanced compared to the original YOLOv3 algorithm, and that the mAP is increased by 0.55%. Compared to the Single Shot MultiBox Detector (SSD) algorithm and Faster Region-based Convolutional Neural Network (Faster RCNN) algorithm, the precision, recall rate, and mAP all performed well, achieving 97.06%, 99.72% and 99.50%, respectively. On this basis, the recognized point symbols are associated with POI, and the coordinate of point symbols are assigned through keyword matching and enrich their attribute information. This enables automatic configuration of point symbols and achieves a relatively good effect of map configuration.
Use of a MODIS Satellite-Based Aridity Index to Monitor Drought Conditions in the Pearl River Basin from 2001 to 2021
Authors: Niu Kunlong , Qiu Junliang , Cai Shirong , Zhang Wenxin , Mu Xiaolin , Park Edward , Yang Xiankun
Published: October 2022

In recent decades, global climate change has made natural hazards increasingly prevalent. Droughts, as a common natural hazard, have been a hot study topic for years. Most studies conducted drought monitoring in arid and semi-arid regions. In humid and sub-humid regions, due to climate change, seasonal droughts and seasonal water shortages were often observed too, but have not been well studied. This study, using a MODIS satellite-based aridity index (SbAI), investigated spatiotemporal changes in drought conditions in the subtropical Pearl River Basin. The study results indicated that the inter-annual SbAI exhibited a significant decreasing trend, illustrating a wetter trend observed in the basin in the past two decades. The decreasing trend in the SbAI was statistically significant in the dry season, but not in the monsoon season. The drought conditions displayed an insignificant expansion in the monsoon season, but exhibited statistically significant shrinking in the dry season. The Pearl River Basin has become wetter over past two decades, probably due to the results of natural impacts and human activities. The areas with increased drought conditions are more likely impacted by human activities such as water withdrawal for irrigation and industrial uses, and fast urbanization and increased impervious surfaces and resultant reduction in water storage capacity. This study provided a valuable reference for drought assessment across the Pearl River Basin.
The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta
Authors: Manojlović-Stojanoski Milica , Borković-Mitić Slavica , Nestorović Nataša , Ristić Nataša , Trifunović Svetlana , Stevanović Magdalena , Filipović Nenad , Stojsavljević Aleksandar , Pavlović Slađan
Published: October 2022

The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.
Combined Application of Aminoglycosides and Ascorbic Acid in the Elimination of Proteus mirabilis Rods Responsible for Causing Catheter-Associated Urinary Tract Infections (CAUTIs)—A Molecular Approach
Authors: Stolarek Paulina , Bernat Przemysław , Różalski Antoni
Published: October 2022

Proteus mirabilis is a common cause of catheter-associated urinary tract infections (CAUTIs). In this study, we verified the effectiveness of amikacin or gentamicin and ascorbic acid (AA) co-therapy in eliminating uropathogenic cells, as well as searched for the molecular basis of AA activity by applying chromatographic and fluorescent techniques. Under simulated physiological conditions, a combined activity of the antibiotic and AA supported the growth (threefold) of the P. mirabilis C12 strain, but reduced catheter colonization (≤30%) in comparison to the drug monotherapy. Slight modifications in the phospholipid and fatty acid profiles, as well as limited (≤62%) 2’,7’-dichlorofluorescein fluorescence, corresponding to the hydroxyl radical level, allowed for the exclusion of the hypothesis that the anti-biofilm effect of AA was related to membrane perturbations of the C12 strain. However, the reduced (≤20%) fluorescence intensity of propidium iodide, as a result of a decrease in membrane permeability, may be evidence of P. mirabilis cell defense against AA activity. Quantitative analyses of ascorbic acid over time with a simultaneous measurement of the pH values proved that AA can be an effective urine acidifier, provided that it is devoid of the presence of urease-positive cells. Therefore, it could be useful in a prevention of recurrent CAUTIs, rather than in their treatment.
Caffeine and Its Antioxidant Properties—It Is All about Dose and Source
Authors: Ősz Bianca-Eugenia , Jîtcă George , Ștefănescu Ruxandra-Emilia , Pușcaș Amalia , Tero-Vescan Amelia , Vari Camil-Eugen
Published: October 2022

Caffeine is the most frequently used substance with a central nervous system stimulant effect, but its consumption is most often due to the intake of foods and drinks that contain it (coffee, tea, chocolate, food supplements with plant extracts of Guarana, Mate herba, Cola nuts). Due to its innocuity, caffeine is a safe xanthine alkaloid for human consumption in a wide range of doses, being used for its central nervous stimulating effect, lipolytic and diuresis-enhancing properties, but also as a permitted ergogenic compound in athletes. In addition to the mechanisms that explain the effects of caffeine on the targeted organ, there are many proposed mechanisms by which this substance would have antioxidant effects. As such, its consumption prevents the occurrence/progression of certain neurodegenerative diseases as well as other medical conditions associated with increased levels of reactive oxygen or nitrogen species. However, most studies that have assessed the beneficial effects of caffeine have used pure caffeine. The question, therefore, arises whether the daily intake of caffeine from food or drink has similar benefits, considering that in foods or drinks with a high caffeine content, there are other substances that could interfere with this action, either by potentiating or decreasing its antioxidant capacity. Natural sources of caffeine often combine plant polyphenols (phenol-carboxylic acids, catechins) with known antioxidant effects; however, stimulant drinks and dietary supplements often contain sugars or artificial sweeteners that can significantly reduce the effects of caffeine on oxidative stress. The objective of this review is to clarify the effects of caffeine in modulating oxidative stress and assess these benefits, considering the source and the dose administered.
The Deadly Toxin Arsenal of the Tree-Dwelling Australian Funnel-Web Spiders
Authors: Cardoso Fernanda C. , Pineda Sandy S. , Herzig Volker , Sunagar Kartik , Shaikh Naeem Yusuf , Jin Ai-Hua , King Glenn F. , Alewood Paul F. , Lewis Richard J. , Dutertre Sébastien
Published: October 2022

Australian funnel-web spiders are amongst the most dangerous venomous animals. Their venoms induce potentially deadly symptoms, including hyper- and hypotension, tachycardia, bradycardia and pulmonary oedema. Human envenomation is more frequent with the ground-dwelling species, including the infamous Sydney funnel-web spider (Atrax robustus); although, only two tree-dwelling species induce more severe envenomation. To unravel the mechanisms that lead to this stark difference in clinical outcomes, we investigated the venom transcriptome and proteome of arboreal Hadronyche cerberea and H. formidabilis. Overall, Hadronyche venoms comprised 44 toxin superfamilies, with 12 being exclusive to tree-dwellers. Surprisingly, the major venom components were neprilysins and uncharacterized peptides, in addition to the well-known ω- and δ-hexatoxins and double-knot peptides. The insecticidal effects of Hadronyche venom on sheep blowflies were more potent than Atrax venom, and the venom of both tree- and ground-dwelling species potently modulated human voltage-gated sodium channels, particularly NaV1.2. Only the venom of tree-dwellers exhibited potent modulation of voltage-gated calcium channels. H. formidabilis appeared to be under less diversifying selection pressure compared to the newly adapted tree-dweller, H. cerberea. Thus, this study contributes to unravelling the fascinating molecular and pharmacological basis for the severe envenomation caused by the Australian tree-dwelling funnel-web spiders.
Lamp1 Deficiency Enhances Sensitivity to α-Synuclein and Oxidative Stress in Drosophila Models of Parkinson Disease
Authors: Rahmani Zohra , Surabhi Satya , Rojo-Cortés Francisca , Dulac Amina , Jenny Andreas , Birman Serge
Published: October 2022

Parkinson disease (PD) is a common neurodegenerative condition affecting people predominantly at old age that is characterized by a progressive loss of midbrain dopaminergic neurons and by the accumulation of α-synuclein-containing intraneuronal inclusions known as Lewy bodies. Defects in cellular degradation processes such as the autophagy-lysosomal pathway are suspected to be involved in PD progression. The mammalian Lysosomal-associated membrane proteins LAMP1 and LAMP2 are transmembrane glycoproteins localized in lysosomes and late endosomes that are involved in autophagosome/lysosome maturation and function. Here, we show that the lack of Drosophila Lamp1, the homolog of LAMP1 and LAMP2, severely increased fly susceptibility to paraquat, a pro-oxidant compound known as a potential PD inducer in humans. Moreover, the loss of Lamp1 also exacerbated the progressive locomotor defects induced by the expression of PD-associated mutant α-synuclein A30P (α-synA30P) in dopaminergic neurons. Remarkably, the ubiquitous re-expression of Lamp1 in a mutant context fully suppressed all these defects and conferred significant resistance towards both PD factors above that of wild-type flies. Immunostaining analysis showed that the brain levels of α-synA30P were unexpectedly decreased in young adult Lamp1-deficient flies expressing this protein in comparison to non-mutant controls. This suggests that Lamp1 could neutralize α-synuclein toxicity by promoting the formation of non-pathogenic aggregates in neurons. Overall, our findings reveal a novel role for Drosophila Lamp1 in protecting against oxidative stress and α-synuclein neurotoxicity in PD models, thus furthering our understanding of the function of its mammalian homologs.
CNS Pericytes Modulate Local T Cell Infiltration in EAE
Authors: Koch Kathrin , Lindner Maren , Fleck Ann-Katrin , Liebmann Marie , Eschborn Melanie , Zondler Lisa , Diéguez-Hurtado Rodrigo , Adams Ralf H. , Meyer zu Hörste Gerd , Zarbock Alexander , Kuhlmann Tanja , Wiendl Heinz , Klotz Luisa
Published: October 2022

Pericytes at the blood–brain barrier (BBB) are located between the tight endothelial cell layer of the blood vessels and astrocytic endfeet. They contribute to central nervous system (CNS) homeostasis by regulating BBB development and maintenance. Loss of pericytes results in increased numbers of infiltrating immune cells in the CNS in experimental autoimmune encephalomyelitis (EAE), the mouse model for multiple sclerosis (MS). However, little is known about their competence to modulate immune cell activation or function in CNS autoimmunity. To evaluate the capacity of pericytes to directly interact with T cells in an antigen-specific fashion and potentially (re)shape their function, we depleted major histocompatibility complex (MHC) class II from pericytes in a cell type-specific fashion and performed T cell-pericyte cocultures and EAE experiments. We found that pericytes present antigen in vitro to induce T cell activation and proliferation. In an adoptive transfer EAE experiment, pericyte-specific MHC II KO resulted in locally enhanced T cell infiltration in the CNS; even though, overall disease course of mice was not affected. Thus, pericytes may serve as non-professional antigen-presenting cells affecting states of T cell activation, thereby locally shaping lesion formation in CNS inflammation but without modulating disease severity.
The Spike Mutants Website: A Worldwide Used Resource against SARS-CoV-2
Authors: Romeo Isabella , Prandi Ingrid Guarnetti , Giombini Emanuela , Gruber Cesare Ernesto Maria , Pietrucci Daniele , Borocci Stefano , Abid Nabil , Fava Anna , Beccari Andrea R. , Chillemi Giovanni , Talarico Carmine
Published: October 2022

A large number of SARS-CoV-2 mutations in a short period of time has driven scientific research related to vaccines, new drugs, and antibodies to combat the new variants of the virus. Herein, we present a web portal containing the structural information, the tridimensional coordinates, and the molecular dynamics trajectories of the SARS-CoV-2 spike protein and its main variants. The Spike Mutants website can serve as a rapid online tool for investigating the impact of novel mutations on virus fitness. Taking into account the high variability of SARS-CoV-2, this application can help the scientific community when prioritizing molecules for experimental assays, thus, accelerating the identification of promising drug candidates for COVID-19 treatment. Below we describe the main features of the platform and illustrate the possible applications for speeding up the drug discovery process and hypothesize new effective strategies to overcome the recurrent mutations in SARS-CoV-2 genome.
Mining Transcriptomic Data to Uncover the Association between CBX Family Members and Cancer Stemness
Authors: Czerwinska Patrycja , Mackiewicz Andrzej Adam
Published: October 2022

Genetic and epigenetic changes might facilitate the acquisition of stem cell-like phenotypes of tumors, resulting in worse patients outcome. Although the role of chromobox (CBX) domain proteins, a family of epigenetic factors that recognize specific histone marks, in the pathogenesis of several tumor types is well documented, little is known about their association with cancer stemness. Here, we have characterized the relationship between the CBX family members’ expression and cancer stemness in liver, lung, pancreatic, and uterine tumors using publicly available TCGA and GEO databases and harnessing several bioinformatic tools (i.e., Oncomine, GEPIA2, TISIDB, GSCA, UALCAN, R2 platform, Enrichr, GSEA). We demonstrated that significant upregulation of CBX3 and downregulation of CBX7 are consistently associated with enriched cancer stem-cell-like phenotype across distinct tumor types. High CBX3 expression is observed in higher-grade tumors that exhibit stem cell-like traits, and CBX3-associated gene expression profiles are robustly enriched with stemness markers and targets for c-Myc transcription factor regardless of the tumor type. Similar to high-stemness tumors, CBX3-overexpressing cancers manifest a higher mutation load. On the other hand, higher-grade tumors are characterized by the significant downregulation of CBX7, and CBX7-associated gene expression profiles are significantly depleted with stem cell markers. In contrast to high-stemness tumors, cancer with CBX7 upregulation exhibit a lower mutation burden. Our results clearly demonstrate yet unrecognized association of high CBX3 and low CBX7 expression with cancer stem cell-like phenotype of solid tumors.
Enhanced Expression of ARK5 in Hepatic Stellate Cell and Hepatocyte Synergistically Promote Liver Fibrosis
Authors: You Yang , Gao Chongqing , Wu Junru , Qu Hengdong , Xiao Yang , Kang Ziwei , Li Jinying , Hong Jian
Published: October 2022

AMPK-related protein kinase 5 (ARK5) is involved in a broad spectrum of physiological and cell events, and aberrant expression of ARK5 has been observed in a wide variety of solid tumors, including liver cancer. However, the role of ARK5 in liver fibrosis remains largely unexplored. We found that ARK5 expression was elevated in mouse fibrotic livers, and showed a positive correlation with the progression of liver fibrosis. ARK5 was highly expressed not only in activated hepatic stellate cells (HSCs), but also in hepatocytes. In HSCs, ARK5 prevents the degradation of transforming growth factor β type I receptor (TβRI) and mothers against decapentaplegic homolog 4 (Smad4) proteins by inhibiting the expression of Smad ubiquitin regulatory factor 2 (Smurf2), thus maintaining the continuous transduction of the transforming growth factor β (TGF-β) signaling pathway, which is essential for cell activation, proliferation and survival. In hepatocytes, ARK5 induces the occurrence of epithelial-mesenchymal transition (EMT), and also promotes the secretion of inflammatory factors. Inflammatory factors, in turn, further enhance the activation of HSCs and deepen the degree of liver fibrosis. Notably, we demonstrated in a mouse model that targeting ARK5 with the selective inhibitor HTH-01-015 attenuates CCl4-induced liver fibrosis in mice. Taken together, the results indicate that ARK5 is a critical driver of liver fibrosis, and promotes liver fibrosis by synergy between HSCs and hepatocytes.
Aging of Liver in Its Different Diseases
Authors: Radonjić Tijana , Dukić Marija , Jovanović Igor , Zdravković Marija , Mandić Olga , Popadić Višeslav , Popović Maja , Nikolić Novica , Klašnja Slobodan , Divac Anica , Todorović Zoran , Branković Marija
Published: October 2022

The proportion of elderly people in the world population is constantly increasing. With age, the risk of numerous chronic diseases and their complications also rises. Research on the subject of cellular senescence date back to the middle of the last century, and today we know that senescent cells have different morphology, metabolism, phenotypes and many other characteristics. Their main feature is the development of senescence-associated secretory phenotype (SASP), whose pro-inflammatory components affect tissues and organs, and increases the possibility of age-related diseases. The liver is the main metabolic organ of our body, and the results of previous research indicate that its regenerative capacity is greater and that it ages more slowly compared to other organs. With age, liver cells change under the influence of various stressors and the risk of developing chronic liver diseases such as non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), alcoholic steatohepatitis (ASH) and hepatocellular carcinoma (HCC) increases. It has been proven that these diseases progress faster in the elderly population and in some cases lead to end-stage liver disease that requires transplantation. The treatment of elderly people with chronic liver diseases is a challenge and requires an individual approach as well as new research that will reveal other safe and effective therapeutic modalities.
Deep-Eutectic-Solvent-Based Mesoporous Molecularly Imprinted Polymers for Purification of Gallic Acid from Camellia spp. Fruit Shells
Authors: Shen Dianling , Yan Yu , Hu Xiaopeng , Zhong Yujun , Li Zhiyang , Guo Yaping , Xie Lianwu , Yuan Deyi
Published: October 2022

To produce antioxidant substances from agricultural waste Camellia spp. fruit shells before their further utilization, gallic acid from five kinds of Camellia spp. fruit shells was separated on specific recognition by deep eutectic solvent molecularly imprinted polymers (DES@MIPs), which were prepared by bulk polymerization using gallic acid as the template and deep eutectic solvents (α-methylacrylic acid and choline chloride) as functional monomers. The optimized DES@MIPs were characterized by scanning electron microscopy, particle size analysis, nitrogen sorption porosimetry, elemental analysis, Fourier transform infrared spectroscopy, and thermal gravimetric analysis. The adsorptive behavior of gallic acid on DES@MIPs was also investigated. The results indicated that DES@MIPs were successfully prepared as mesoporous materials with average pore diameter of 9.65 nm and total pore volume of 0.315 cm3 g−1, and the adsorption behavior was multilayer adsorption and pseudo-second-order kinetics with the saturation adsorptive capacity of gallic acid reaching 0.7110 mmol g−1. Although the content of gallic acid in five fruit shells was quite different, the purification recovery of gallic acid was high, ranging from 87.85–96.75% with a purity over 80%. Thus, the purification of gallic acid from Camellia spp. fruit shells could be realized feasibly using DES@MIPs with favorable economic and environmental benefits.