bluuebunny/crossref_metadata_2025 huggingface dataset:
(4Z)-Lachnophyllum Lactone, an Acetylenic Furanone from Conyza bonariensis, Identified for the First Time with Allelopathic Activity against Cuscuta campestris
Authors: Fernández-Aparicio Mónica , Soriano Gabriele , Masi Marco , Carretero Pilar , Vilariño-Rodríguez Susana , Cimmino Alessio
Published: May 2022

Cuscuta species are obligate parasitic plants that infect the stems of a wide range of hosts including many crop and weed species causing severe agricultural problems. Using in vitro experiments to screen organic extracts prepared from fifteen autotrophic weed species found in agricultural fields infested with Cuscuta campestris, we have identified for the first time a strong phytotoxic activity in Conyza bonariensis extract against C. campestris. Additional pot experiments revealed that seven day-old Cuscuta seedlings had reduced capacity to coil and properly attach on Conyza plants, leading to reduced parasitic weed infection. Via activity-guided fractionation of Conyza extracts, we isolated and identified the acetylenic furanone (4Z)-lachnophyllum lactone as the major active component, with a concentration required to achieve reduction of 50% Cuscuta seedling growth (IC50) of 24.8 µg/mL. The discovery of (4Z)-lachnophyllum lactone bioactivity could aid the development of efficient and sustainable management strategies for C. campestris, whose control is limited or non-existent.
Proposed Light Wavelengths during Healing of Grafted Tomato Seedlings Enhance Their Adaptation to Transplant Shock
Authors: Melissas Christos , Bantis Filippos , Dangitsis Christodoulos , Kostas Stefanos , Koukounaras Athanasios
Published: May 2022

Tomato, which is mainly established with grafted seedlings, is one of the most popular vegetables worldwide with a high nutritional value,. Market demand for grafted seedlings is high in specific seasons; thus, commercial nurseries face a problem of limited space availability during the healing stage. Light quality is an essential parameter during healing that can adjust seedling development towards desirable traits and lead to time and space saving during seedling production. Moreover, transplant shock constitutes another challenge that could limit crop yield. The objective of this study was to evaluate the overall quality of grafted tomato seedlings and their potential adjustment to transplant shock as affected by different light spectra during healing in a chamber. Evaluations were conducted immediately after exiting the healing chamber and after transplantation into pots. Light wavelengths were used from fluorescent lamps (FL) or light-emitting diodes with red (R), blue (B), red–blue combinations with 12 and 24% blue (12B and 24B), and white (W) emitting 11% blue. W enhanced the dry shoot biomass and the root architecture before and after transplantation. 24B led to an increased stem diameter, root development, and phenolic and antioxidant accumulation at both phases of the experiment. 12B enhanced the leaf area before transplantation and root development after transplantation. FL, R and B induced inferior seedling growth compared to the red–blue-containing LEDs, with B performing poorly in almost all tested parameters. Overall, red, including 11–24% blue, provides the optimum light conditions during the healing stage for the production of high-quality grafted tomato seedlings, with advanced capabilities of abiotic stress adaptation to transplant shock.
3D Assessment of Vine Training Systems Derived from Ground-Based RGB-D Imagery
Authors: Moreno Hugo , Bengochea-Guevara José , Ribeiro Angela , Andújar Dionisio
Published: May 2022

In the field of computer vision, 3D reconstruction of crops plays a crucially important role in agriculture. On-ground assessment of geometrical features of vineyards is of vital importance to generate valuable information that enables producers to take the optimum actions in terms of agricultural management. A training system of vines (Vitis vinifera L.), which involves pruning and a trellis system, results in a particular vine architecture, which is vital throughout the phenological stages. Pruning is required to maintain the vine’s health and to keep its productivity under control. The creation of 3D models of vineshoots is of crucial importance for management planning. Volume and structural information can improve pruning systems, which can increase crop yield and improve crop management. In this experiment, an RGB-D camera system, namely Kinect v2, was used to reconstruct 3D vine models, which were used to determine shoot volume on eight differentiated vineyard training systems: Lyre, GDC (Geneva Double Curtain), Y-Trellis, Pergola, Single Curtain, Smart Dyson, VSP (Vertical Shoot Positioned), and the head-trained Gobelet. The results were compared with dry biomass ground truth-values. Dense point clouds had a substantial impact on the connection between the actual biomass measurements in four of the training systems (Pergola, Curtain, Smart Dyson and VSP). For the comparison of actual dry biomass and RGB-D volume and its associated 3D points, strong linear fits were obtained. Significant coefficients of determination (R2 = 0.72 to R2 = 0.88) were observed according to the number of points connected to each training system separately, and the results revealed good correlations with actual biomass and volume values. When comparing RGB-D volume to weight, Pearson’s correlation coefficient increased to 0.92. The results reveal that the RGB-D approach is also suitable for shoot reconstruction. The research proved how an inexpensive optical sensor can be employed for rapid and reproducible 3D reconstruction of vine vegetation that can improve cultural practices such as pruning, canopy management and harvest.
Grass Pea (Lathyrus sativus L.)—A Sustainable and Resilient Answer to Climate Challenges
Authors: Gonçalves Letice , Rubiales Diego , Bronze Maria R. , Vaz Patto Maria C.
Published: May 2022

Grass pea (Lathyrus sativus L.) is an annual cool-season grain legume widely cultivated in South Asia, Sub-Saharan Africa, and in the Mediterranean region. It is a stress-resilient crop with high nutritional value, considered a promising source of traits to breed for adaptation/mitigation of climate change effects. It is also reported as a suitable crop for more sustainable production systems such as intercropping. In this review, we elaborate an integrative perspective including not only an agronomic-based but also a variety-breeding-based strategy in grass pea to deal with climate change impacts, summarizing the current knowledge on grass pea biotic/abiotic stress resistance. Additionally, we highlight the importance of implementing fundamental techniques to create diversity (as interspecific hybridization or gene editing) and increase genetic gains (as speed breeding or the efficient identification of breeding targets via genomics) in the development of multiple stress-resistant varieties that simultaneously provide yield and quality stability under climate vulnerable environments.
Co-Inoculations with Plant Growth-Promoting Bacteria in the Common Bean to Increase Efficiency of NPK Fertilization
Authors: Mortinho Emariane Satin , Jalal Arshad , da Silva Oliveira Carlos Eduardo , Fernandes Guilherme Carlos , Pereira Nathália Cristina Marchiori , Rosa Poliana Aparecida Leonel , do Nascimento Vagner , de Sá Marco Eustáquio , Teixeira Filho Marcelo Carvalho Minhoto
Published: May 2022

Given the hypothesis that co-inoculation with plant growth-promoting bacteria (PGPB) enhances the beneficial effects of Rhizobium tropici with greater mineral nutrition, optimizes biological nitrogen fixation and reduces use of fertilizers in bean plants, the objective of this research was to evaluate the synergistic effects of Rhizobium tropici associated with Azospirillum brasilense, Bacillus subtilis, Pseudomonas fluorescens and their combinations, on increasing the efficiency of NPK fertilization to obtain high winter yields of the (irrigated) common bean in the Cerrado region. The experiment was carried out in the field over two years in a Rhodic Hapludox under a no-till system in Selvíria, Brazil. The experimental design comprised complete randomized blocks with four replications in a 3 × 7 factorial scheme. The treatments consisted of three doses of NPK fertilizer (control—0 kg ha−1 (control); 50% of the recommended dose; 100% of the recommended dose in two parts) and seven doses of inoculation or co-inoculation (control; Rhizobium tropici; R. tropici + Azospirillum brasilense; R. tropici + Bacillus subtilis; R. tropici + Pseudomonas fluorescens; R. tropici + A. brasilense + B. subtilis; R. tropici + A. brasilense + P. fluorescens). The PGPB in the co-inoculations increased the hundred-grain weight, the grain pod−1, the grain plant−1 and the grain yield following the NPK doses. The grain yield of the common bean was increased by co-inoculation with R. tropici + A. brasilense + P. fluorescens without NPK treatments, co-inoculation with R. tropici + P. fluorescens and R. tropici + A. brasilense + B. subtilis with the 50% dose of NPK and co-inoculation with R. tropici + B. subtilis with the recommended dose of NPK fertilizer (100%).
Application of Biocat G, Selenium, and Chitosan to Counteract the Negative Effects of Cd in Broccoli Plants Grown in Soilless Culture
Authors: Pérez-Millán Rafael , Cámara-Zapata Jose Maria , Fernández-Zapata Juan Carlos , Simón-Grao Silvia , Alfosea-Simón Marina , Zavala-González Ernesto Alejandro , Shahid Muhammad Adnan , García-Sánchez Francisco
Published: May 2022

The accumulation of cadmium in plants produces phytotoxic damage and a decrease in crop yield. To avoid this effect, it is necessary to prevent its absorption by roots and reduce its toxicity in plant tissues. The current study was aimed to evaluate the effect of the exogenous applications of Biocat G (fulvic/humic acids), selenium (Se), and chitosan to roots and leaves of broccoli plants exposed to Cd stress. The applied treatments were: (i) T1: Hoagland nutrient solution (NS), (ii) T2: NS + Cd at 3 mg L-1 (NS + Cd), (iii) T3: NS + Cd + root application of Biocat G (NS + Cd + BioG), (iv) T4: NS + Cd + foliar application of Se (NS + Cd + Se1), (v) T5: NS + Cd + root application of Se (NS + Cd + Se2), (vi) T6: NS + Cd + foliar application of chitosan (NS + Cd + chitosan1), and (vii) T7: NS + Cd + root application of chitosan (NS + Cd + chitosan2). The results showed that the exogenous application of Biocat G and Se (T3 and T5) ameliorated the adverse effects caused by Cd toxicity and significantly improved plant growth rate by decreasing Cd toxicity; besides, Biocat G was able to limit the transport of Cd from the leaves to the inflorescences, reducing the content of Cd in the edible part. These treatments (T3 and T5) yielded the best results, act on the plants by deactivating Cd toxicity, but they did not affect its accumulation in the plant tissue. In addition, Biocat G limits the transport of Cd from the non-edible to the edible part.
Effects of Ground Cover Management, Landscape Elements and Local Conditions on Carabid (Coleoptera: Carabidae) Diversity and Vine Vitality in Temperate Vineyards
Authors: Porter Lauren , Khalil Sarhan , Forneck Astrid , Winter Silvia , Griesser Michaela
Published: May 2022

Sustainable vineyard management in inter-rows may improve biodiversity and ecosystem service provision in landscapes with a high density of vineyards. The current work investigates the effect of three inter-row ground cover treatments (bare soil by tillage, alternating and complete vegetation cover) on carabid beetle communities and vine vitality, in relation to climatic, soil and landscape parameters. Pitfall traps were used to collect carabids in the spring and autumn of 2016 from nine Austrian vineyards, with all three ground cover treatments established in each vineyard. Additionally, grape berry samples were collected before harvest in order to determine juice quality parameters. Generalized linear mixed models revealed that complete vegetation cover, the most extensive vineyard inter-row management, decreased both carabid density and species richness. The variables hours of sunshine, vineyard cover at the landscape scale and mesofauna abundance had negative impacts on species richness. The largest differentiator of carabid communities was the sampling timepoint, and we observed clustering associated to vineyard manager, whereas ground cover treatment played no significant role. The importance of treatment on vine vitality parameters was low; however, complete vegetation cover was detrimental to vine vegetative growth and berry weight. On the basis of our results, we conclude that although community composition may be influenced by pedo-climatic conditions and landscape components, alternating vegetation cover is an option for maintaining both carabid diversity and high-quality berries in vineyards.
Overexpression of OsPHT1;4 Increases Phosphorus Utilization Efficiency and Improves the Agronomic Traits of Rice cv. Wuyunjing 7
Authors: Hu Zhi , Huang Xu , Wang Xiaowen , Xia Huihuang , Liu Xiuli , Sun Yafei , Sun Shubin , Hu Yibing , Cao Yue
Published: May 2022

Inorganic phosphate (Pi) is taken up by plant roots and translocated via phosphate transporters. Previously, we showed that phosphate transporter OsPHT1;4 in the PHT1 family participates in phosphate acquisition and mobilization; it facilitates the embryo development of Japonica rice Nipponbare. This study investigated the potential of manipulating the expression of OsPHT1;4 to increase Pi acquisition efficiency and crop productivity in rice cv. Wuyunjing 7 (WYJ 7), a cultivar widely grown in Yangtze River Delta of China. The OsPHT1;4 overexpression lines and wild-type WYJ 7 were treated under different Pi conditions in hydroponic and field experiments. Quantitative real-time RT-PCR analysis and the transgenic plants expressing GUS reporter gene indicate strong expression of OsPHT1;4 in roots and leaf collars of cv. WYJ 7. The total P contents in shoots of the OsPHT1;4-overexpressing plants were significantly higher under Pi-deficient hydroponic conditions than the wild type under Pi sufficiency and deficiency. 33Pi uptake and translocation assays confirmed the results. In the field condition, OsPHT1;4 overexpression lines had a higher P concentration in tissues than the wild type control, and the panicle performance of the overexpression lines including the grain yield was improved as well. Taken together, our results show that OsPHT1;4 plays an important role in the acquisition and mobilization of Pi in WYJ 7, especially under Pi deficiency. The study highlights the importance of OsPHT1;4 in improving the agronomic traits of the widely grown rice cultivar in China.
Alternative Mulches for Sustainable Greenhouse Tomato Production
Authors: Marín-Guirao Jose Ignacio , Martín-Expósito Emilio , García-García María del Carmen , de Cara-García Miguel
Published: May 2022

Soil mulching has advantages for horticultural crops, from both agronomic and phytosanitary points of view. The most common material used is polyethylene (PE); however, promising alternatives from the circular economy exist, such as straw (ST) and biodegradable biopolymers (BBs). The effect of the three aforementioned mulches was evaluated and compared to non-mulched soil in a Mediterranean greenhouse for two years of an organic tomato crop. Physical (moisture and temperature) and physicochemical properties of the soil, in addition to crop yield and the effect of the mulches on weed control, were assessed. Additionally, the deterioration of plastic mulches was assessed. The temperature was higher in the mulched soils, but few differences were found between soil and BB at the end of the second cycle. Evaporation was lower in mulched soil, in general, without big differences among the types of mulch. Crop yield did not show differences. At the end of the trials, of the 16 physicochemical variables evaluated, only a slight increase in pH was detected in the ST-mulched plots. BB film degradation reached 5.6% and 6.7% of the total surface at the end of the first and second cycles, respectively. Weeds were equally limited for PE, BB, and ST mulches, but cereal seeds contained within the straw germinated randomly all over the crop cycle. In summary, straw and biodegradable plastic mulches offered the same benefits as conventional PE mulch. Therefore, they can be considered a feasible and more sustainable option, in addition to being consistent with the principles of the bioeconomy.
Sublethal Effects of Three Insecticides on Development and Reproduction of Spodoptera frugiperda (Lepidoptera: Noctuidae)
Authors: Wu Hui-Ming , Feng Hang-Li , Wang Guo-Di , Zhang Li-Li , Zulu Lovemore , Liu Ya-Hui , Zheng Yong-Li , Rao Qiong
Published: May 2022

Spodoptera frugiperda is a serious invasive pest, which has attracted attention globally in recent years. Research on the sublethal effects of common insecticides on S. frugiperda is important for its comprehensive management in the field. In this paper, the sublethal effects have been studied for S. frugiperda exposed to the LC30 concentration of three insecticides, chlorantraniliprole, dinotefuran, and beta-cypermethrin. The results showed that the pupation rates of the treatment groups were significantly lower than that of control group, but there were no significant differences in the eclosion rate. Chlorantraniliprole and beta-cypermethrin significantly inhibited fecundity of the F0 and F1 generations, and the number of eggs laid per female decreased by 67.4% and 43.1%, respectively, and that of the F1 generation decreased by 28.0% and 21.7%, respectively. The intrinsic growth rate (rm), net growth rate (R0), and weekly growth rate (λ) of the F0 generation in the chlorantraniliprole and beta-cypermethrin treatment groups were significantly lower than those in the control group. Additionally, dinotefuran had a certain role in promoting oviposition in the F0 and F1 generations, which may stimulate the growth of offspring population and cause the resurgence of pests. The results provide a reference for the effective implementation of the IPM plan in the field to control S. frugiperda.
Biochemical Analyses of Ten Cyanobacterial and Microalgal Strains Isolated from Egyptian Habitats, and Screening for Their Potential against Some Selected Phytopathogenic Fungal Strains
Authors: Senousy Hoda H. , El-Sheekh Mostafa M. , Saber Abdullah A. , Khairy Hanan M. , Said Hanan A. , Alhoqail Wardah. A. , Abu-Elsaoud Abdelghafar M.
Published: May 2022

Microalgae and cyanobacteria are rich sources of numerous phytochemical compounds with intrinsic antifungal potential. This research aimed to screen the phytochemical compounds and contents, as well as the antioxidant profiles, in eight cyanobacterial and two microalgal strains isolated from soil and brackish water habitats in Egypt. Our study also evaluated their antifungal activities against three phytopathogenic fungi—namely, Pythium ultimum, Fusarium solani, and Botryodiplodia theobromae, which are known to cause severe plant loss. The biochemical compounds were obtained from the cyanobacterial and algal methanolic extracts, and were identified through comparative phytochemical analyses related to the inhibition of the fungal pathogens. Comparative qualitative analyses of alkaloids, steroids, glycosides, and saponins were also carried out. The quantitative phytochemical screening of the cyanobacterial and algal strains investigated revealed the presence of xylanase, glucanase, and chitinase enzymes, along with some bioactive compounds, such as phenolics, flavonoids, proteins, neutral sugars, and carotenoids, which were species-dependent and detected in variable amounts in the extracts. The unicellular green microalgal strain Dunaliella sp. HSSASE13 displayed the highest level of antioxidant activity. However, the highest antifungal activities were shown by the heterocystous cyanobacterial strain Anabaena sp. HSSASE11 (83.94%), followed by Dunaliella sp. HSSASE13 (81.94%) and the non-heterocystous cyanobacterial strain Oscillatoria nigro-viridis HSSASE 15 (63.42%), against the three fungal pathogens B. theobromae, F. solani, and P. ultimum, respectively. Our results indicate that the highest significant and positive correlations of flavonoids (r = 0.854), phenolics (r = 0.785), DPPH scavenging activity (r = 0.876), total proteins (r = 0.808), xylanase activity (r = 0.876), glucanase activity (r = 0.746), and total neutral sugars (r = 0.764), in terms of their antifungal activities, were recorded against F. solani. Conclusively, the cyanobacterial and algal strains tested in the present study can be useful agents for the management and biocontrol of plant-infecting fungal pathogens.
A Case Study in Desertified Area: Soybean Growth Responses to Soil Structure and Biochar Addition Integrating Ridge Regression Models
Authors: Ma Hua , Li Qirui , Egamberdieva Dilfuza , Bellingrath-Kimura Sonoko Dorothea
Published: May 2022

Desertified land covers one-fourth of the world’s total land area. Meeting the high food demands in areas affected by desertification is a major problem. This case study provided fundamental information to demonstrate the potential for utilizing the desertified land. The soybean trial was established in two sandy clay loam soils (desertified land) and one silty clay loam soil. Two types of biochar were applied as treatments. We aimed to investigate the response of soybean plants to soil structure, soil nutrient condition, and biochar amendment in the two types of soil. In addition, ridge regression was employed to model the plant growth indicators by soil structure, soil nutrients condition, soil water content, and biochar amendment. We conclude that (1) overall soil productivity in sandy clay loam soil is lower than in silty clay loam soil. The sandy clay loam soil may have high efficacy for crop production due to its higher harvest index. (2) Aggregate size 0.5–1 mm, 1–2 mm, and 2–3 mm indicated more important in plant biomass formation in silty clay loam soil. The low aggregate stability of sandy clay loam soil made the field more vulnerable to wind erosion in the semi-arid monsoon climate. (3) Cob biochar and wood biochar increased soybean shoot biomass by 48.7% and 45.0% in silty clay loam soil. (4) The higher N-fixing ability of nodules in sandy clay loam soil indicates an advantage to reduce the use of N-fertilizers in desertified areas. (5) Exponential polynomial regression ameliorated the accuracy of prediction of plant growth indicators in comparison to linear regression.
The Course of Physiological Processes, Yielding, and Grain Quality of Hybrid and Population Wheat as Affected by Integrated and Conventional Cropping Systems
Authors: Jańczak-Pieniążek Marta , Buczek Jan , Kwiatkowski Cezary A. , Harasim Elżbieta
Published: May 2022

At present, under the conditions of climate change, for mainly environmental but also economic reasons, especially in the case of new wheat genotypes, alternative cropping systems are recommended in addition to the common conventional system. The aim of this study was to determine the effect of the integrated system (INTEG) and conventional system (CONV) on the physiological parameters, yield, and mineral composition of the grain, as well as the amount and quality of protein of winter wheat Hymalaya (hybrid cv.) and Formacja (population cv.) against the background of changing hydrothermal conditions in the years of the study. The field experiment was carried out in 2016–2019 in Przecław (50°11′00″ N, 21°29′00″ E), Poland. More favorable values of physiological parameters and grain yield were found in the CONV system than in the INTEG system. A more efficient course of the photosynthesis process in cv. Hymalaya effected a higher grain yield, which was similar in the INTEG system to that of cv. Formacja from the CONV system. The use of the CONV system effected an increase in the grain quality traits as well as the sum of gliadins and glutenins, including the subunits γ gliadins, LMW glutenins, and HMW glutenins. Grain of cv. Hymalaya from the INTEG system had higher contents of Fe, Mn, and Mg and more favorable composition of glutenin proteins and their HMW/LMW ratio than cv. Formacja. Higher values of quality traits and gluten protein fractions and subunits, along with a reduction in the grain yield of wheat cultivars, were favored by periods with rainfall deficit in the wheat ripening period, where low hydrothermal coefficients were recorded.
Modeling of Chilled/Supercooled Pork Storage Quality Based on the Entropy Weight Method
Authors: Zhao Songsong , Lin Hengxun , Li Shuangqing , Liu Chenghao , Meng Junhong , Guan Wenqiang , Liu Bin
Published: May 2022

The entropy weight method (EWM) was developed and used to integrate multiple quality indexes of pork to generate a comprehensive measure of quality. The Arrhenius equation and chemical kinetic reaction were used to fit and generate the shelf life prediction model. The pork was stored at the temperatures of 7 °C, 4 °C, 1 °C and −1 °C. Quality indexes, such as drip loss, color, shear force, pH, TAC, TVB-N and TBARS were measured. The results show that low temperatures effectively delay microbial growth and lipid oxidation. The regression coefficients (R2) for the comprehensive scores at each temperature were greater than 0.973 and the activation energy Ea was 9.7354 × 104 kJ mol−1. The predicted shelf life of pork stored at 7 °C, 4 °C, 1 °C and −1 °C was 4.35 d, 6.85 d, 10.88 d and 14.90 d, respectively. In conclusion, EWM is an effective method to predict the shelf life of chilled/supercooled pork.
Predicting Antibiotic Effect of Vancomycin Using Pharmacokinetic/Pharmacodynamic Modeling and Simulation: Dense Sampling versus Sparse Sampling
Authors: Kim Yong Kyun , Lee Jae Ha , Jang Hang-Jea , Zang Dae Young , Lee Dong-Hwan
Published: May 2022

This study aimed to investigate the effect of a structural pharmacokinetic (PK) model with fewer compartments developed following sparse sampling on the PK parameter estimation and the probability of target attainment (PTA) prediction of vancomycin. Two- and three-compartment PK models of vancomycin were used for the virtual concentration–time profile simulation. Datasets with reduced blood sampling times were generated to support a model with a lesser number of compartments. Monte Carlo simulation was conducted to evaluate the PTA. For the two-compartment PK profile, the total clearance (CL) of the reduced one-compartment model showed a relative bias (RBias) and relative root mean square error (RRMSE) over 90%. For the three-compartment PK profile, the CL of the reduced one-compartment model represented the largest RBias and RRMSE, while the steady-state volume of distribution of the reduced two-compartment model represented the largest absolute RBias and RRMSE. A lesser number of compartments corresponded to a lower predicted area under the concentration–time curve of vancomycin. The estimated PK parameters and predicted PK/PD index from models built with sparse sampling designs that cannot support the PK profile can be significantly inaccurate and unprecise. This might lead to the misprediction of the PTA and selection of improper dosage regimens when clinicians prescribe antibiotics.
Antibiotic Use, Incidence and Risk Factors for Orthopedic Surgical Site Infections in a Teaching Hospital in Madhya Pradesh, India
Authors: Skender Kristina , Machowska Anna , Singh Vivek , Goel Varun , Marothi Yogyata , Lundborg Cecilia Stålsby , Sharma Megha
Published: May 2022

Orthopedic surgeries contribute to the overall surgical site infection (SSI) events worldwide. In India, SSI rates vary considerably (1.6–38%); however, there is a lack of a national SSI surveillance system. This study aims to identify the SSI incidence, risk factors, antibiotic prescription and susceptibility patterns among operated orthopedic patients in a teaching hospital in India. Data for 1205 patients were collected from 2013 to 2016. SSIs were identified based on the European Centre for Disease Prevention and Control guidelines. The American Society for Anesthesiologists classification system was used to predict patients’ operative risk. Univariable and multivariable backward stepwise logistic regressions were performed. Overall, 7.6% of patients developed SSIs over three years. The most common SSIs causative microorganism was Staphylococcus aureus (7%), whose strains were resistant to penicillin (100%), erythromycin (80%), cotrimoxazole (80%), amikacin (60%) and cefoxitin (60%). Amikacin was the most prescribed antibiotic (36%). Male sex (OR 2.64; 95%CI 1.32–5.30), previous hospitalization (OR 2.15; 95%CI 1.25–3.69), antibiotic prescription during hospitalization before perioperative antibiotic prophylaxis (OR 4.19; 95%CI 2.51–7.00) and postoperative length of stay > 15 days (OR 3.30; 95%CI 1.83–5.95) were identified as significant risk factors. Additionally, preoperative shower significantly increased the SSI risk (OR 4.73; 95%CI 2.72–8.22), which is unconfirmed in the literature so far.
In Vivo Assessment of Antioxidant Potential of Human Milk Treated by Holder Pasteurization or High Hydrostatic Pressure Processing: A Preliminary Study on Intestinal and Hepatic Markers in Adult Mice
Authors: Wemelle Eve , Marousez Lucie , Lesage Jean , De Lamballerie Marie , Knauf Claude , Carneiro Lionel
Published: May 2022

Preterm infants are highly susceptible to oxidative stress due to an imbalance between endogenous oxidant and antioxidant systems. In addition, these newborns are frequently fed with donor milk (DM) treated by Holder pasteurization (HoP) at 62.5 °C for 30 min, which is known to alter numerous heat-sensitive factors, including some antioxidants. High hydrostatic pressure (HHP) processing was recently proposed as an innovative method for the treatment of DM. The present study aimed to measure the redox balance of HoP- and HHP-DM and to study, in vivo, the effects of HoP- and HHP-DM on the gut and liver. H2O2, vitamin A and vitamin E (α- and γ-tocopherols) concentrations, as well as the total antioxidant capacity (TAC), were measured in raw-, HoP- and HHP-DM. The gene expression level of antioxidant systems and inflammatory response were quantified in the ileum and liver of adult mice after 7 days of oral administration of HoP- or HHP-DM. HoP reduced the γ-tocopherol level, whereas HHP treatment preserved all vitamins close to the raw milk level. The milk H2O2 content was reduced by HHP but not by HoP. The total antioxidant capacity of DM was reduced after HHP processing measured by PAOT-Liquid® technology but was unaffected after measurement by ORAC assay. In mice, HHP-DM administration induced a stimulation of antioxidant defenses and reduced some inflammatory markers in both the ileum and liver compared to HoP-DM treatment. Our preliminary study suggests that the HHP processing of DM may better protect preterm infants from gut and liver pathologies compared to HoP, which is currently used in most human milk banks.
N-Acetylcysteine Enhances the Recovery of Ischemic Limb in Type-2 Diabetic Mice
Authors: Zhu Qiang , Liu Xuanyou , Zhu Qingyi , Liu Zehao , Yang Chunlin , Wu Hao , Zhang Linfang , Xia Xiujuan , Wang Meifang , Hao Hong , Cui Yuqi , Zhang Guangsen , Hill Michael A. , Flaker Gregory C. , Zhou Shenghua , Liu Zhenguo
Published: May 2022

Critical limb ischemia (CLI) is a severe complication of diabetes mellitus that occurs without effective therapy. Excessive reactive oxygen species (ROS) production and oxidative stress play critical roles in the development of diabetic cardiovascular complications. N-acetylcysteine (NAC) reduces ischemia-induced ROS production. The present study aimed to investigate the effect of NAC on the recovery of ischemic limb in an experimental model of type-2 diabetes. TALLYHO/JngJ diabetic and SWR/J non-diabetic mice were used for developing a CLI model. For NAC treatment, mice received NAC (1 mg/mL) in their drinking water for 24 h before initiating CLI, and continuously for the duration of the experiment. Blood flow, mechanical function, histology, expression of antioxidant enzymes including superoxide dismutase (SOD)-1, SOD-3, glutathione peroxidase (Gpx)-1, catalase, and phosphorylated insulin receptor substrate (IRS)-1, Akt, and eNOS in ischemic limb were evaluated in vivo or ex vivo. Body weight, blood glucose, plasma advanced glycation end-products (AGEs), plasma insulin, insulin resistance index, and plasma TNF-a were also evaluated during the experiment. NAC treatment effectively attenuated ROS production with preserved expressions of SOD-1, Gpx-1, catalase, phosphorylated Akt, and eNOS, and enhanced the recovery of blood flow and function of the diabetic ischemic limb. NAC treatment also significantly decreased the levels of phosphorylated IRS-1 (Ser307) expression and plasma TNF-α in diabetic mice without significant changes in blood glucose and AGEs levels. In conclusion, NAC treatment enhanced the recovery of blood flow and mechanical function in ischemic limbs in T2D mice in association with improved tissue redox/inflammatory status and insulin resistance.
Antioxidative Effects of Standardized Aronia melanocarpa Extract on Reproductive and Metabolic Disturbances in a Rat Model of Polycystic Ovary Syndrome
Authors: Rudic Jovan , Jakovljevic Vladimir , Jovic Nikola , Nikolic Maja , Sretenovic Jasmina , Mitrovic Slobodanka , Bolevich Sergey , Bolevich Stefani , Mitrovic Miroslav , Raicevic Sasa , Andric Kristina , Dimkic Milenkovic Andjela , Rakic Dejana , Joksimovic Jovic Jovana
Published: May 2022

Polycystic ovary syndrome (PCOS) represents the most common endocrinopathy among childbearing-age women, with oxidative stress (OS) underlying its etiopathogenesis. Metformin (MET) represents a frequently used agent in PCOS. However, weak results encourage alternative treatments. We aimed to investigate isolated and synergistic effects of Standardized Aronia melanocarpa extract (SEA) and MET for alleviating reproductive and metabolic PCOS abnormalities. PCOS induction was followed by 28-day treatment with MET, SAE, or MET + SEA. Bodyweight (BW), cyclicity, histological, and ultrasonographical ovarian analyses were performed. Hormonal, glycemic, and lipid profiles were accessed, as well as systemic and ovarian oxidative status; BW, cyclicity, ovarian histomorphology, ovarian volume, testosterone and progesterone levels, as well as LDL, triglycerides, and total cholesterol levels were aggravated after PCOS-induction and improved after MET, SEA, and MET + SEA treatment. MET + SEA had the greatest impact on glycoregulation. Alterations in OS parameters (TBARS, O2−, H2O2, catalase, superoxide dismutase, and reduced glutathione) could be responsible for observed differences; (4) Conclusions: Our findings confirmed that SAE alone or along with MET was capable of ameliorating reproductive and metabolic disturbances in the PCOS rat model, with the restoration of OS parameters. SAE alone did not alter the protective effects of MET in PCOS.
The Effects of Dietary Arthrospira platensis on Oxidative Stress Response and Pigmentation in Yellow Catfish Pelteobagrus fulvidraco
Authors: Liu Cui , Liu Haokun , Zhu Xiaoming , Han Dong , Jin Junyan , Yang Yunxia , Xie Shouqi
Published: May 2022

In aquaculture, fish are often exposed to several stress conditions, which will cause oxidative disorder and bring about health and quality problems. Arthrospira platensis contains abundant bioactive ingredients, which are beneficial for animal health. This study was conducted to investigate the effects of A. platensis on pigmentation, antioxidant capacity, and stress response after air exposure of fish. A total of 120 yellow catfish Pelteobagrus fulvidraco (initial weight 70.19 ± 0.13 g) were divided into three tanks per treatment and fed diets supplemented with 0 g kg−1 A. platensis (CON) and 20 g kg −1 A. platensis (AP) for 65 days. The results indicated that dietary A. platensis had no effects on the growth of yellow catfish. The AP diet significantly reduced lactic acid (LD) and cortisol levels stimulated by air exposure stress (p < 0.05). Dietary A. platensis significantly increased plasma superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities and glutathione (GSH) contents, and the relative expression levels of sod and cat, to protect against oxidative stress caused by air exposure (p < 0.05). The AP diet significantly improved the relative expression level of nrf2 (nuclear factor erythroid-2 related factor 2), while the relative expression level of keap1 (kelch-like ECH associated protein 1) was downregulated, and the protein levels of liver Nrf2 were significantly increased after air exposure stimuli (p < 0.05). Dietary A. platensis significantly increased skin lutein contents, increased skin redness, yellowness and chroma (p < 0.05), and improved body color abnormalities after oxidative stress caused by air exposure stimuli. Skin yellowness was associated with lutein contents and the expression levels of some antioxidant genes to varying degrees. Overall, dietary A. platensis could be utilized as a feed additive to activate the antioxidant response, as well as alleviate oxidative stress and pigmentation disorder induced by air exposure.
GNSS/INS Integration Based on Machine Learning LightGBM Model for Vehicle Navigation
Authors: Li Bangxin , Chen Guangwu , Si Yongbo , Zhou Xin , Li Pengpeng , Li Peng , Fadiji Tobi
Published: May 2022

To solve the problem of data accuracy degradation of vehicle GNSS/INS integrated navigation systems when the GNSS signal is unavailable or there is a GNSS outage, this paper improves the existing GNSS/INS integration methodology for land vehicle navigation based on the AI method. First, a GNSS/INS integration methodology for land vehicle navigation based on position update architecture (PUA) using LightGBM regression for predicting the position of a vehicle during a GNSS outage is presented. It uses LightGBM to model the relationship between INS data and vehicle position changes. On-board INS and GNSS data are collected when the GNSS signal is available and are used to train the PUA-LightGBM model; in the event of a GNSS outage, INS data are used as the input to the PUA-LightGBM to predict the change in vehicle position. Second, a vehicle navigation data acquisition system was designed for model validation. This included a self-developed GNSS/INS integrated navigation system and a Novatel pwrpak7-e1 GNSS/INS integrated navigation system for data acquisition on six road segments. Finally, the collected data were used for machine learning training of the PUA-LightGBM model and the existing PUA-RandomForest model. As a result, the PUA-LightGBM predicts the vehicle position with less error in the event of a GNSS outage and takes less time to train. It was also demonstrated that by allowing the model to be dynamically trained or updated while the vehicle is moving the PUA-LightGBM could adapt perfectly to the predictions of vehicle position changes in different complex road segments.
In Silico and In Vitro Assessment of Antimicrobial and Antibiofilm Activity of Some 1,3-Oxazole-Based Compounds and Their Isosteric Analogues
Authors: Apostol Theodora-Venera , Chifiriuc Mariana Carmen , Nitulescu George Mihai , Olaru Octavian Tudorel , Barbuceanu Stefania-Felicia , Socea Laura-Ileana , Pahontu Elena Mihaela , Karmezan Crina Maria , Marutescu Luminita Gabriela
Published: May 2022

In this paper, we report on the antimicrobial activity assessment of 49 compounds previously synthesized as derivatives of alanine or phenylalanine that incorporate a 4-(4-X-phenylsulfonyl)phenyl fragment (X = H, Cl, or Br), namely 21 acyclic compounds (6 × N-acyl-α-amino acids, 1 × N-acyl-α-amino acid ester, and 14 × N-acyl-α-amino ketones) and 28 pentatomic heterocycles from the oxazole-based compound class (6 × 4H-1,3-oxazol-5-ones, 16 × 5-aryl-1,3-oxazoles, and 6 × ethyl 1,3-oxazol-5-yl carbonates). Both in silico and in vitro qualitative and quantitative assays were used to investigate the antimicrobial potential of these derivatives against planktonic and biofilm-embedded microbial strains. Some of the tested compounds showed promising antimicrobial and antibiofilm activity depending on their chemical scaffold and lipophilic character.
Vulnerability Analysis of LTE-R Train-to-Ground Communication Time Synchronization
Authors: Chen Yong , Zhan Zhixian , Niu Kaiyu
Published: May 2022

The time synchronization of LTE-R train-to-ground communication systems plays an important role in ensuring the safety of high-speed railways. In the LTE-R time synchronization process, existing problems, such as the time synchronization message broadcast address and LTE-R all-IP architecture, are vulnerable to attack. In order to analyze the impact of these problems, we propose a new vulnerability analysis method of LTE-R time synchronization based on stochastic Petri nets. Firstly, we construct a stochastic Petri net model of an LTE-R time synchronization process under attack. Secondly, steady-state probability expressions are obtained using the model isomorphism Markov chain. Finally, bychanging the firing rate of several key vulnerable nodes, the relationship curve between the firing rate and the steady-state probability of the clock node is obtained. Simulations show that the vulnerability of LTE-R time synchronization is most affected by the attack on eNodeB of the LTE-R base station. The results can provide a certain theoretical basis for the evolution of high-speed railway GSM-R communication systems to LTE-R.
Fractional Flow Reserve (FFR) Estimation from OCT-Based CFD Simulations: Role of Side Branches
Authors: Gamage Peshala T. , Dong Pengfei , Lee Juhwan , Gharaibeh Yazan , Zimin Vladislav N. , Bezerra Hiram G. , Wilson David L. , Gu Linxia
Published: May 2022

The computational fluid dynamic method has been widely used to quantify the hemodynamic alterations in a diseased artery and investigate surgery outcomes. The artery model reconstructed based on optical coherence tomography (OCT) images generally does not include the side branches. However, the side branches may significantly affect the hemodynamic assessment in a clinical setting, i.e., the fractional flow reserve (FFR), defined as the ratio of mean distal coronary pressure to mean aortic pressure. In this work, the effect of the side branches on FFR estimation was inspected with both idealized and optical coherence tomography (OCT)-reconstructed coronary artery models. The electrical analogy of blood flow was further used to understand the impact of the side branches (diameter and location) on FFR estimation. Results have shown that the side branches decrease the total resistance of the vessel tree, resulting in a higher inlet flowrate. The side branches located at the downstream of the stenosis led to a lower FFR value, while the ones at the upstream had a minimal impact on the FFR estimation. Side branches with a diameter larger than one third of the main vessel diameter are suggested to be considered for a proper FFR estimation. The findings in this study could be extended to other coronary artery imaging modalities and facilitate treatment planning.
Performance of Artificial Intelligence Models Designed for Diagnosis, Treatment Planning and Predicting Prognosis of Orthognathic Surgery (OGS)—A Scoping Review
Authors: Khanagar Sanjeev B. , Alfouzan Khalid , Awawdeh Mohammed , Alkadi Lubna , Albalawi Farraj , Alghilan Maryam A.
Published: May 2022

The technological advancements in the field of medical science have led to an escalation in the development of artificial intelligence (AI) applications, which are being extensively used in health sciences. This scoping review aims to outline the application and performance of artificial intelligence models used for diagnosing, treatment planning and predicting the prognosis of orthognathic surgery (OGS). Data for this paper was searched through renowned electronic databases such as PubMed, Google Scholar, Scopus, Web of science, Embase and Cochrane for articles related to the research topic that have been published between January 2000 and February 2022. Eighteen articles that met the eligibility criteria were critically analyzed based on QUADAS-2 guidelines and the certainty of evidence of the included studies was assessed using the GRADE approach. AI has been applied for predicting the post-operative facial profiles and facial symmetry, deciding on the need for OGS, predicting perioperative blood loss, planning OGS, segmentation of maxillofacial structures for OGS, and differential diagnosis of OGS. AI models have proven to be efficient and have outperformed the conventional methods. These models are reported to be reliable and reproducible, hence they can be very useful for less experienced practitioners in clinical decision making and in achieving better clinical outcomes.
Machine Learning-Based Models for Detection of Biomarkers of Autoimmune Diseases by Fragmentation and Analysis of miRNA Sequences
Authors: Ali Nehal M. , Shaheen Mohamed , Mabrouk Mai S. , Aborizka Mohamed
Published: May 2022

Thanks to high-throughput data technology, microRNA analysis studies have evolved in early disease detection. This work introduces two complete models to detect the biomarkers of two autoimmune diseases, multiple sclerosis and rheumatoid arthritis, via miRNA analysis. Based on work the authors published previously, both introduced models involve complete pipelines of text mining methods, integrated with traditional machine learning methods, and LSTM deep learning. This work also studies the fragmentation of miRNA sequences to reduce the needed processing time and computational power. Moreover, this work studies the impact of obtaining two different library preparation kits (NEBNEXT and NEXTFLEX) on the detection accuracy for rheumatoid arthritis. Additional experiments are applied to the proposed models based on three different transcriptomic datasets. The results denote that the transcriptomic fragmentation model reported a biomarker detection accuracy of 96.45% on a sequence fragment size of 0.2, indicating a significant reduction in execution power while retaining biomarker detection accuracy. On the other hand, the LSTM model obtained a promising detection accuracy of 72%, implying savings in feature engineering processing. Additionally, the fragmentation model and the LSTM model reported 22.4% and 87.5% less execution time than work in the literature, respectively, denoting a considerable execution power reduction.
Antidepressant-Like Effect of Traditional Medicinal Plant Carthamus Tinctorius in Mice Model through Neuro-Behavioral Tests and Transcriptomic Approach
Authors: Alegiry Mohamed H. , El Omri Abdelfatteh , Bayoumi Ahmed Atef , Alomar Mohammed Y. , Rather Irfan A. , Sabir Jamal S. M.
Published: May 2022

Major depression disorder (MDD) has become a common life-threatening disorder. Despite the number of studies and the introduced antidepressants, MDD remains a major global health issue. Carthamus tinctorius (safflower) is traditionally used for food and medical purposes. This study investigated the chemical profile and the antidepressant-like effect of the Carthamus tincto-rius hot water extract in male mice and its mechanism using a transcriptomic analysis. The antidepressant effect of hot water extract (50 mg/kg and 150 mg/kg) was investigated in mice versus the untreated group (saline) and positive control group (fluoxetine 10 mg/kg). Hippocampus transcriptome changes were investigated to understand the Carthamus tinctorius mechanism of action. The GC-MS analysis of Carthamus tinctorius showed that hot water extract yielded the highest amount of oleamide as the most active ingredient. Neuro-behavioral tests demonstrated that the safflower treatment significantly reduced immobility time in TST and FST and improved performance in the YMSAT compared to the control group. RNA-seq analysis revealed a significant differential gene expression pattern in several genes such as Ube2j2, Ncor1, Tuba1c, Grik1, Msmo1, and Casp9 related to MDD regulation in 50 mg/kg safflower treatment as compared to untreated and fluoxetine-treated groups. Our findings demonstrated the antidepressant-like effect of safflower hot water extract and its bioactive ingredient oleamide on mice, validated by a significantly shortened immobility time in TST and FST and an increase in the percentage of spontaneous alternation.