bluuebunny/crossref_metadata_2025 huggingface dataset:
Acceptability of Edible Food Packaging in Slovakia: A Case Study on Young Generation
Authors: Horská Elena , Šedík Peter , Mušinská Kristína , Savitskaya Tatsiana , Grinshpan Dmitrij , Kačániová Miroslava
Published: December 2021

The increasing problem of plastic pollution from food packaging created a new market potential for inventing environmental-friendly packaging in the food market including edible food packaging. The main objective of the study was to identify attitudes and perceptions of young people toward edible food packaging. Primary research was conducted via a questionnaire survey. The research sample involved 22 respondents between 19 and 30 years from Slovakia. A questionnaire survey was interconnected with sensory analysis where respondents tested 23 samples of edible food packaging. The results showed that consumers perceive as the most important aspect of food packaging the possibilities of their recycling, followed by the protective function and ecological aspect, while the least important aspect was the design of the packaging. The best rating based on the taste was obtained by sample no. 22 (Mentha × Piperita) while the worst rating was obtained by sample no. 3 (Zingiber officinale). The majority of tested edible food packaging had an indifferent taste.
The Impact of Preoperative Nutritional Status on the Survival of Patients With Esophageal Squamous Cell Carcinoma
Authors: Chen Shao-bin , Liu Di-tian , Chen Yu-ping
Published: December 2021

Background: The goal of this study was to investigate the impact of different nutritional parameters in patients with esophageal squamous cell carcinoma (ESCC) who underwent surgical resection.

Methods: A total of 620 patients with ESCC who underwent esophagectomy were analyzed. A receiver operating characteristic curve was constructed to set the appropriate cutoff points for five nutritional parameters: serum albumin (SA), body mass index (BMI), geriatric nutritional risk index (GNRI), prognostic nutritional index (PNI), and a new modified nutritional risk index (mNRI). Survival analyses were performed to calculate overall survival and investigate the independent prognostic factors.

Results: The median preoperative BMI, SA, GNRI, PNI, and mNRI values were 20.90, 42.75, 102.95, 51.90, and 63.90, respectively. The corresponding optimal cutoff points were 18.75 for BMI, 43.05 for SA, 98.5 for GNRI, 51.45 for PNI, and 61.45 for mNRI. All nutritional parameters were significantly correlated with tumor length and pT category. Decreased nutritional parameters were significantly correlated with poor survival in univariate analysis; however, only the mNRI was an independent prognostic factor in multivariate analysis (P = 0.041).

Conclusions: Nutritional parameters are convenient and valuable prognostic factors in ESCC patients who undergo surgical resection. The new mNRI parameter may be superior to the other nutritional parameters.
Development of a Recombinase Polymerase Amplification Fluorescence Assay for the Detection of Canine Adenovirus 2
Authors: Xiao Li , Zhang Mengdi , Tian Zhige , Ge Ye , Zhang Tongyuan , Yi Li , Cong Feng
Published: December 2021

Canine adenovirus type 2 (CAdV-2) is often found in co-infections with other pathogens causing canine infectious respiratory disease (CIRD). Rapid, efficient, and convenient pathogen detection is the best approach for early confirmatory diagnosis. In this study, we developed and evaluated a rapid real-time recombinase polymerase amplification (RPA) assay for detection of canine adenovirus 2 (CAV), which can detect CAV within 15 min at 39°C. The detection limit that assay was 214 copies/μl DNA molecules per reaction. The specificity was indicated by a lack of cross-reaction with canine distemper virus (CDV), canine coronavirus (CCV), and canine parvovirus (CPV). Field and clinical applicability of this assay were evaluated using 86 field samples. The coincidence rate of the detection results for clinical samples between CAV-RPA and qPCR was 97.7%. In summary, the real-time CAV-RPA analysis provides an efficient, rapid and sensitive detection method for CAV.
Genomic Rearrangement and Recombination of Porcine Circovirus Type 2 and Porcine Circovirus-Like Virus P1 in China
Authors: Wen Libin , He Kongwang
Published: December 2021

Porcine circovirus type 2 (PCV2) belongs to the genus Circovirus of the family Circoviridae, and it has been associated with porcine circovirus (associated) disease (PCVD or PCVAD) in pigs. PCVAD is the generic term for a series of disease syndromes that have caused economic losses to the pig industry worldwide. Since the discovery of PCV2 in the late 1990s, the virus has continued to evolve, and novel genotypes have continued to appear. Moreover, there has been recombination between different genotypes of PCV2. This review attempts to illustrate some progress concerning PCV2 in genome rearrangement and genomic recombination with non-PCV2-related nucleic acids, particularly focusing on the porcine circovirus-like virus P1 formed by the recombination of PCV2. The presence of rearranged PCV2 genomes can be demonstrated both in vivo and in vitro, and these subviral molecules ranged from 358 to 1,136 bp. Depending on whether it has the ability to encode a protein, the agents formed by PCV2 recombination can be divided into two categories: porcine circovirus-like viruses and porcine circovirus-like mini agents. We mainly discuss the porcine circovirus-like virus P1 regarding genomic characterization, etiology, epidemiology, and pathogenesis. Further research needs to be conducted on the pathogenicity of other porcine circovirus-like viruses and porcine circovirus-like mini agents and the effects of their interactions with PCV2, especially for the porcine circovirus-like mini agents that do not have protein-coding functions in the genome.
An Automated Canine Line-Up for Detection Dog Research
Authors: Aviles-Rosa Edgar O. , Gallegos Shawna F. , Prada-Tiedemann Paola A. , Hall Nathaniel J.
Published: December 2021

Currently, there is a need to develop technology that facilitates and improves detection dog research. The aim of this research was to develop an automated computer-driven olfactory line-up task. The apparatus consisted of three olfactometers. Each olfactometer was equipped with flow meters to regulate air flow and dilution and six solenoid valves connected to odor jars. Each olfactometer generated an odor which was carried to an odor port where the dogs sample it. The olfactometer's valves were activated by a microcontroller, and a Python program was built to control each olfactometer and randomize and balance the odor presentation. Dogs (N = 12) received one or two 40-trial training sessions in a day where they progressed through a series of training phases where they learned to detect and alert to double-base smokeless powder (SP). An “alert” consisted of a 4-s nose hold. This was measured by infrared sensors in the ports. For each trial, the apparatus recorded dogs' search latency, sniff time, port entries, and response. All this information was automatically recorded in a csv file. A photoionization detector (PID) and solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME-GC/MS) were used to evaluate the odor dynamics and to instrumentally verify odor presence and clearance. A control test was conducted at the end of the training to ensure dogs were alerting exclusively to the odorant. All 12 dogs readily learned to operate the apparatus within 23 days, and all exceeded 85% accuracy. Control tests indicated dogs were leveraging only olfactory cues and not unintentional cues such as auditory cues from the apparatus. Analytical data showed that odor was detected in the port immediately after the activation of a valve and that odor clearance occurred immediately after the valve was closed. The apparatus developed was easy to operate by the dogs and allowed substantial data collection using double-blind testing procedures in a very short period at an affordable cost point for research equipment (~$5,000 USD). The apparatus may prove to be a useful research tool to provide optimal odor stimuli control, ensure double-blind conditions, reduce labor, and significantly increase the amount of data collected.
Multi-Mode Interferometry: Application to TiO2–SiO2 Sol-Gel Waveguide-Based Sensing in the Aerospace Domain
Authors: Royon Maxime , Blanchet Thomas , Adnan Muhammad , Jamon Damien , Royer François , Vocanson Francis , Marin Emmanuel , Morana Adriana , Boukenter Aziz , Ouerdane Youcef , Jourlin Yves , Evenblij Rolf , Van Leest Thijs , Wankhade Aditya , De Smet Marie-Anne , Atherton Kathryn , Girard Sylvain
Published: December 2021

The optimization of a 2D optical sensor based on TiO2–SiO2 sol-gel waveguides for damage detection in the aerospace domain was performed in the framework of the ADD-ON European project. The sensor is based on the transportation of visible light along numerous waveguides, and damage is detected and localized through the monitoring of the output light from the waveguide grid. In this work, we have developed an architecture, inspired by a multi-mode interferometer (MMI), allowing us to efficiently multiply the number of waveguides that can be probed by a single optical source. For this, the beam propagation method (BPM) was used to model a rectangular MMI coupler (40 × 5624 µm2) operating in the visible region (600 nm), ensuring the propagation of light into three waveguides. The conceived device was then manufactured by UV photolithography (direct laser writing technique). The simulations and experimental results show that light transport into this architecture allows for the successful simultaneous probing of three waveguides. By complexifying the device structure, successful MMI couplers were easily manufactured, allowing us to probe 9, 15, or 45 TiO2–SiO2 waveguides with a unique light source. Finally, a further investigation regarding 24 consecutive thermal cycles from −40 °C to 60 °C, representative of the temperature changes during aircraft cruising, was performed. This study reveals that TiO2–SiO2 sol-gel waveguides are not mechanically damaged by temperature changes, while the light guidance remains unaffected, confirming that this sensor is very promising for aerospace applications. Since a single source can monitor several guides, the production of more compact, low-cost, and less intrusive sensors can be achieved by fulfilling structural health monitoring requirements.
Influence of Variety and Nitrogen Fertilization on the Technological Parameters of Special Malts Prepared from Naked and Hulled Oat Varieties
Authors: Błażewicz Józef , Kawa-Rygielska Joanna , Leszczyńska Danuta , Grabiński Jerzy , Gasiński Alan
Published: December 2021

Grains of four naked oat varieties (Amant, Maczo, Polar and Siwek) and one hulled oat variety (Kozak) from three consecutive years (2018, 2019 and 2020), grown under three different nitrogen fertilization regimes (40, 60 and 80 kg of nitrogen per hectare) were malted and then mashed in the laboratory conditions for the first time; this was carried out to determine whether hulled and naked oat grains possess favourable properties as a raw material for the production of malt. Most of the analysed samples possess a favourable Kolbach index (39.06–46.82%), good extractivity (81.07–81.97%) and rather good saccharification time (13.33–26.67 min); however, some disadvantages of the produced malts could be pointed out. During the congress mashing, the filtration time of the worts produced from the hulled and naked oat malts was long (96.67–110 min) and the wort volume was not satisfactory (155–228.53 mL). Subsequent studies over oat malting and mashing need to be performed to maximize oat potential as the raw material for the production of the malt.
Effects of Nitrogen Rates on the Productivity and Nutritive Value of Forage Grass Grown under Extreme Climatic Conditions
Authors: Marcinkevičienė Aušra , Velička Rimantas , Kosteckas Robertas , Rudinskienė Aušra , Adamonytė Inga , Kriaučiūnienė Zita
Published: December 2021

This vegetative experiment was carried out at the greenhouse of Vytautas Magnus University Agriculture Academy Open Access Joint Research Centre of Agriculture and Forestry (Lithuania) in 2020–2021. The aim of these studies was to determine the effect of different nitrogen rates on the productivity and nutritional quality of forage grasses (a mixture of red clover and timothy) under the most common extremes of climate change, i.e., soil moisture deficiency and surplus. Under drought and waterlogging stresses, fertilization of the red clover and timothy mixture with high N rates was ineffective. The clover and timothy mixture recovery after drought took 21 days. The aboveground dry biomass of the clover and timothy mixture grown under drought conditions was significantly lower by 36.3 to 47.2% compared to that formed under optimum soil moisture and waterlogging conditions. The root biomass of forage grass mixtures was lowest under drought conditions when fertilized at the highest N rate (N25+120). The aboveground biomass of clover grown under different soil moisture conditions depended on the number of plants (r2 = 0.78, p < 0.01) and assimilating leaf area (r2 = 0.83, p < 0.01), and that of timothy on the number of vegetative tillers (r2 = 0.46, p < 0.05). Under drought simulation conditions, increasing the N rate increased the crude protein and crude fibre contents in the aboveground biomass of the clover and timothy mixture, while the crude ash content decreased.
Daisychain: Search and Interactive Visualisation of Homologs in Genome Assemblies
Authors: Schliebs Oliver , Chan Chon-Kit Kenneth , Bayer Philipp E. , Petereit Jakob , Singh Ajit , Hassani-Pak Keywan , Batley Jacqueline , Edwards David
Published: December 2021

Daisychain is an interactive graph visualisation and search tool for custom-built gene homology databases. The main goal of Daisychain is to allow researchers working with specific genes to identify homologs in other annotation releases. The gene-centric representation includes local gene neighborhood to distinguish orthologs and paralogs by local synteny. The software supports genome sequences in FASTA format and GFF3 formatted annotation files, and the process of building the homology database requires a minimum amount of user interaction. Daisychain includes an integrated web viewer that can be used for both data analysis and data publishing. The web interface extends KnetMaps.js and is based on JavaScript.
Germinated Chickpea and Lupin as Promising Ingredients for Breadmaking—Rheological Features
Authors: Atudorei Denisa , Ungureanu-Iuga Mădălina , Codină Georgiana Gabriela , Mironeasa Silvia
Published: December 2021

Improving the alpha-amylase activity of wheat flour represents an opportunity to valorize wheat grains of low baking quality. In this sense, germinated legumes can be used to increase enzymatic activity, giving superior final product characteristics at the same time. The aim of this study was to underline the effects of chickpea (CGF) and lupin germinated flours (LGF) added simultaneously to white wheat flour on the rheological behavior of dough and to evaluate an optimal product microstructure. For this purpose, the falling number, dough rheological properties during mixing, 3D-deformation and fermentation, and the visco-elastic behavior were evaluated, the effects of factors (CGF and LGF levels) and their optimization have been studied by applying a full factorial design and response surface methodology (RSM). The LGF sample had a composition of 39.4% protein, 10.3% moisture, 6.9% fat, and 3.4% ash, whereas the CGF presented 21.1 % protein, 9.4% moisture, 5.2% fat, and 3.6% ash. The results showed that CGF and LGF determined the decrease of the falling number, dough water absorption, tolerance to kneading, dough consistency at 250 and 450 s, extensibility, the maximum height of the gas release curve, volume of gas retained by the dough at the end of the test, total volume of CO2 production, visco-elastic moduli, and gelatinization temperatures. On the other hand, dough elasticity and alveograph curve ratio increased proportionally to the increase of CGF and LGF addition levels. The optimal combination considering the rheological properties of dough was found to be 8.57% CGF, 5.31% LGF, and 86.12% wheat flour, with enhanced alpha-amylase activity being obtained compared to the control. These results provide valuable information on the possibility of using germinated legumes such as chickpeas and lupin in breadmaking to enhance wheat flour technological properties (besides traditionally used barley malt flour).
The Effect of Titanium Organic Complex on Pollination Process and Fruit Development of Apple cv. Topaz
Authors: Bieniasz Monika , Konieczny Anna
Published: December 2021

Optimal weather conditions are necessary for the proper course of the flowering process and high activity of pollinators, which is the most important factor affecting pollination. Because weather conditions do not always favour effective pollination and because pollinators’ activities are decreasing, the application of compound stimulating pollination and fertilization may be a good perspective for increasing yield. Titanium is considered a beneficial element for plants. Preliminary studies have indicated the positive effect of titanium organic complex on pollen adhesion to the stigma and pollen germination on the stigma of tomato and cucumber. Therefore, a 2-year experiment was designed to determine the effect of titanium organic complex application on the pollination process and fruit development of apple cv. Topaz (Malus domestica Borkh.) The experiment demonstrated the positive effect of titanium organic complex on fertilization of ovules because of effective pollination, seed setting, and fruit development of apple. Application of titanium organic complex improved pollen adhesion to the stigma and pollen germination on the stigma. In addition, titanium organic complex increased the number of pollen tubes growing through the pistil style, which resulted in fertilization that was more effective, as confirmed by the higher number of seeds set in fruits. Higher numbers of seeds set in fruits positively affected their weight and size. Therefore, fruit harvested from trees to which titanium organic complex was applied were characterized by greater weight, length, and diameter compared to fruits obtained from the trees to which titanium organic complex was not applied.
Agronomic Efficiency Losses by Ammonia Emission from Staple Crops in China as Response to Various Mitigation Strategies: A Meta-Analysis Study
Authors: Abdo Ahmed I. , Sun Daolin , El-Sobky El-Sayed E. A. , Wei Hui , Zhang Jiaen
Published: December 2021

Economic losses and environmental hazards are meaningful problems of emitted ammonia induced by extensive use of synthetic nitrogen fertilizers. The concept presented as yield scaled fertilizer productivity (YSFP) in our meta-analysis in addition to nitrogen use efficiency (NUE), nitrogen agronomic efficiency (NAE), and productivity of applied nitrogen (PAN) were used to weight ammonia emission (AV)-induced reductions in wheat, maize, and rice production. The comprehensive meta-analysis was used to weight the reductions in these parameters by AV as the difference between observed means of the collected studies and their adjusted means using AV factor. There were higher reductions in agronomic production induced by AV in rice than maize and wheat. AV-induced reductions in PAN of rice, maize, and wheat were decreased by 4.99, 3.71, and 2.42 (kg grains kg−1 N), respectively. YSFP and PAN recorded the highest sensitivity to AV in wheat (R2 = 0.88 for both) and rice (R2 = 0.92 and 0.89, respectively), while NUE was the most efficient parameter in weighting AV induced agronomic effects in maize (R2 = 0.81). Slow-released N fertilizers resulted in the lowest reductions in AV induced agronomic losses followed by organic amendments and then urea while using other synthetic fertilizers recorded the highest reductions by 3.90, 6.40, 1.41, and 4.70 in YSFP, NUE, NAE, and PAN, respectively. Inhibitors had the highest effect on mitigating AV induced agronomic losses compared with biochar and mulching and affected the parameters following that order as percentages of no amendments, YSFP (52.63%) > PAN (47.18%) > NUE (40.83%) > NAE (38.75%). This study outlines the reductions in agronomic production induced by AV and weights the efficiency of various mitigation strategies under various agronomic conditions. The results proved the efficiency of YSFP with NUE parameters to weight the effect of AV on crop yield, while suggesting to find out more applicable parameters in further studies.
Browse from Three Tree Legumes Increases Forage Production for Cattle in a Silvopastoral System in the Southwest Amazon
Authors: Dablin Lucy , Lewis Simon L. , Milliken William , Monro Alexandre , Lee Mark A.
Published: December 2021

Assessing the palatability of forage from locally adapted trees could improve the sustainability of livestock production systems. However, grasses continue to dominate livestock feed across the Amazon. We established a silvopastoral cattle farming system in Peru, comparing three different forage tree species with grass monocultures using a randomised block design. Trees were arranged in alleys of 0.5 × 7.5 m, planted alongside grass, and were directly browsed by cattle. Browse removal was estimated by three methods: destructive sampling, canopy measurements and leaf counts. We found that all three tree species were palatable to cattle. Plots containing trees and grass produced more available forage (mean > 2.2 Mg ha−1) for cattle than the grass monocultures (mean = 1.5 Mg ha−1). Destructive sampling below 1.6 m demonstrated that cattle consumed 99% of the available Erythrina berteroana forage, 75% of the available Inga edulis forage and 80% of the available Leucaena leucocephala forage in 8 days. This research demonstrates methodologies to estimate the intake of locally adapted browse species by cattle and highlights the potential benefits of silvopastoral systems in the Amazon. Planting trees could also benefit animal health and provide ecosystem services such as soil regeneration, enhanced nutrient cycling and carbon capture.
Dynamics of Known Long Non-Coding RNAs during the Maternal-to-Zygotic Transition in Rabbit
Authors: Shi Yu , Cai Mingcheng , Du Kun , Bai Xue , Tang Lipeng , Jia Xianbo , Chen Shiyi , Wang Jie , Lai Songjia
Published: December 2021

The control of pre-implantation development in mammals undergoes a maternal-to-zygotic transition (MZT) after fertilization. The transition involves maternal clearance and zygotic genome activation remodeling the terminal differentiated gamete to confer totipotency. In the study, we first determined the profile of long non-coding RNAs (lncRNAs) of mature rabbit oocyte, 2-cell, 4-cell, 8-cell, and morula embryos using RNA-seq. A total of 2673 known rabbit lncRNAs were identified. The lncRNAs exhibited dynamic expression patterns during pre-implantation development. Moreover, 107 differentially expressed lncRNAs (DE lncRNAs) were detected between mature oocyte and 2-cell embryo, while 419 DE lncRNAs were detected between 8-cell embryo and morula, consistent with the occurrence of minor and major zygotic genome activation (ZGA) wave of rabbit pre-implanted embryo. This study then predicted the potential target genes of DE lncRNAs based on the trans-regulation mechanism of lncRNAs. The GO and KEGG analyses showed that lncRNAs with stage-specific expression patterns promoted embryo cleavage and synchronic development by regulating gene transcription and translation, intracellular metabolism and organelle organization, and intercellular signaling transduction. The correlation analysis between mRNAs and lncRNAs identified that lncRNAs ENSOCUG00000034943 and ENSOCUG00000036338 may play a vital role in the late-period pre-implantation development by regulating ILF2 gene. This study also found that the sequential degradation of maternal lncRNAs occurred through maternal and zygotic pathways. Furthermore, the function analysis of the late-degraded lncRNAs suggested that these lncRNAs may play a role in the mRNA degradation in embryos via mRNA surveillance pathway. Therefore, this work provides a global view of known lncRNAs in rabbit pre-implantation development and highlights the role of lncRNAs in embryogenesis regulation.
Administration of Dietary Microalgae Ameliorates Intestinal Parameters, Improves Body Weight, and Reduces Thawing Loss of Fillets in Broiler Chickens: A Pilot Study
Authors: Šefcová Miroslava Anna , Santacruz Francisco , Larrea-Álvarez César Marcelo , Vinueza-Burgos Christian , Ortega-Paredes David , Molina-Cuasapaz Gabriel , Rodríguez Jessica , Calero-Cáceres William , Revajová Viera , Fernández-Moreira Esteban , Larrea-Álvarez Marco
Published: December 2021

This pilot investigation aimed at studying the feasibility of using a low dose (0.2%) of dietary microalgae as a means of improving intestinal morphometry, body weight, and selected meat quality parameters in broilers. A total of 72 one-day-old ROSS 308 male chicks were randomly separated into four groups; three experimental pens in which the birds were fed with biomass from Tysochrysis lutea, Tetraselmis chuii, and Porphyridium cruentum over 30 days and a control group. T. chuii and P. cruentum had a positive effect with regard to body weight. In treated animals, duodenal and ileal sections showed characteristic tall and thin villi, with serrated surfaces and goblet cell differentiation. In both sections, values of the villus-height-to-crypt-depth ratio were increased by microalgae ingestion. The thawing weight loss of fillets was reduced in T. chuii-fed animals. The positive effects exerted by T. chuii and P. cruentum on intestinal architecture were associated with the improved body weight. Arguably, these outcomes exhibit the potential of using these species to enhance growth performance in broiler chickens by promoting gut homeostasis and thus nutrient absorption.
Sharp Interface Capturing in Compressible Multi-Material Flows with a Diffuse Interface Method
Authors: Nandan Shambhavi , Fochesato Christophe , Peybernes Mathieu , Motte Renaud , De Vuyst Florian
Published: December 2021

Compressible multi-materialflows are encountered in a wide range of natural phenomena and industrial applications, such as supernova explosions in space, high speed flows in jet and rocket propulsion, underwater explosions, and vapor explosions in post accidental situations in nuclear reactors. In the numerical simulations of these flows, interfaces play a crucial role. A poor numerical resolution of the interfaces could make it difficult to account for the physics, such as material separation, location of the shocks and contact discontinuities, and transfer of the mass, momentum and heat between different materials/phases. Owing to such importance, sharp interface capturing remains an active area of research in the field of computational physics. To address this problem in this paper we focus on the Interface Capturing (IC) strategy, and thus we make use of a newly developed Diffuse Interface Method (DIM) called Multidimensional Limiting Process-Upper Bound (MLP-UB). Our analysis shows that this method is easy to implement, can deal with any number of material interfaces, and produces sharp, shape-preserving interfaces, along with their accurate interaction with the shocks. Numerical experiments show good results even with the use of coarse meshes.
Role of the Mn-Catalase in Aerobic Growth of Lactobacillus plantarum ATCC 14431
Authors: Peacock Trent , Hassan Hosni M.
Published: December 2021

Lactobacilli are Gram-positive aerotolerant organisms that comprise the largest genus of Lactic Acid Bacteria (LAB). Most lactobacilli are devoid of the antioxidant enzymes, superoxide dismutases, and catalases, required for protection against superoxide radicals and hydrogen peroxide (H2O2), respectively. However, some lactobacilli can accumulate millimolar concentrations of intracellular manganese and spare the need for superoxide dismutase, while others possess non-heme catalases. L. plantarum is associated with plant materials and plays an important role in fermented foods and gut microbiomes. Therefore, understanding the effects of the environment on the growth and survival of this organism is essential for its success in relevant industrial applications. In this report, we investigated the physiological role of Mn-catalase (MnKat) in Lactobacillus plantarum ATCC 14431. To this end, we compared the physiological and morphological properties of a ΔMnkat mutant strain and its isogenic parental strain L. plantarum ATCC 14431. Our data showed that the MnKat is critical for the growth of L. plantarum ATCC 14431 in the presence of oxygen and resistance to H2O2. The aerobic growth of the mutant in presence or absence of H2O2 was improved in the Mn-rich medium (APT) as compared to the growth in MRS medium. Furthermore, under aerobic conditions the mutant strain possessed atypical cellular morphology (i.e., shorter, and fatter). In conclusion, the MnKat of L. plantarum ATCC 14431 is important for aerobic growth, protection against H2O2, and maintenance of the rod-shaped cell morphology under aerobic conditions.
Environmental Benefits of Ultra-Low Emission (ULE) Technology Applied in China
Authors: Jiao Xiaomiao , Ni Ruijing , Chen Lulu , Adeniran Jamiu Adetayo , Weng Hongjian , Wang Jingxu , Chen Youfan , Ren Shihua , Liu Xiao
Published: December 2021

Seven scenarios were designed to study the national environmental benefits of ULE in coal-fired power plants (CPPs), ULE in industrial coal burning (ICB) and NH3 emission reduction by using the GEOS-Chem model. The results showed that although the CPPs have achieved the ULE transformation target, the PM2.5 concentration across the country has decreased by 4.8% (1.4 μg/m3). Due to the complex non-linear chemical competition mechanism among nitrate and sulfate, the average concentration of nitrate in the country has increased by 1.5% (0.1 μg/m3), which has reduced the environmental benefits of the power plant emission reduction. If the ULE technology is applied to the ICB to further reduce NOx and SO2, although the PM2.5 concentration can be reduced by 10.1% (2.9 μg/m3), the concentration of nitrate will increase by 2.7% (0.2 μg/m3). Based on the CPPs-ULE, NH3 emissions reduced by 30% and 50% can significantly reduce the concentration of ammonium and nitrate, so that the PM2.5 concentration is decreased by 11.5% (3.3 μg/m3) and 16.5% (4.7 μg/m3). Similarly, based on the CPPs-ICB-ULE, NH3 emissions can be reduced by 30% and 50% and the PM2.5 concentration reduced by 15.6% (4.4 μg/m3) and 20.3% (5.8 μg/m3). The CPPs and ICB use the ULE technology to reduce NOx and SO2, thereby reducing the concentration of ammonium and sulfate, causing the PM2.5 concentration to decline, and NH3 reduction is mainly achieved through reducing the concentration of ammonium and nitrate to reduce the concentration of PM2.5. In order to better reduce the concentration of PM2.5, NOx, SO2 and NH3 emission reduction control measures should be comprehensively considered in different regions of China. By comprehensively considering the economic cost and environmental benefits of ULE in ICB and NH3 emission reduction, an optimal haze control scheme can be determined.
Adsorption of Gas-Phase Cyclohexanone on Atmospheric Water Films
Authors: Hao Shangpeng , Sun Chao , Zhang Yuanpeng , Wang Haitao , Zhao Wenbo , Wang Xiaolu , Li Jinghai
Published: December 2021

The fate of atmospheric volatile organic compounds (VOCs) strongly depends on the partitioning processes on the surface of aerosols, which are coated with a thin water film. However, the behavior of VOCs in the aqueous film of aerosols is difficult to measure. In this work, the interfacial partition constant of cyclohexanone was determined using a novel flow-tube reactor. A thin, aqueous film placed in the reactor was exposed to cyclohexanone gas. The subsequent partitioning was measured using chromatography techniques. The quality control tests were first conducted to ensure the accuracy of the adsorption experiments. The cyclohexanone concentration was then plotted as a function of film thickness to obtain the partitioning constants. As the thickness of the water film decreased, the aqueous concentration of cyclohexanone increased, indicating that surface adsorption played a dominant role in the uptake of cyclohexanone. According to the temperature dependence of the interfacial partition constant, the solvation enthalpy and entropy of cyclohexanone were obtained. The results of this study would help to elucidate the effect of atmospheric water film on the gas–aerosol partitioning of VOCs, and thus can help to better understand the fate of VOCs in the atmosphere.
Wiman’s Type Inequality in Multiple-Circular Domain
Authors: Kuryliak Andriy , Skaskiv Oleh
Published: December 2021

In the paper we prove for the first time an analogue of the Wiman inequality in the class of analytic functions f∈A0p(G) in an arbitrary complete Reinhard domain G⊂Cp, p∈N represented by the power series of the form f(z)=f(z1,⋯,zp)=∑‖n‖=0+∞anzn with the domain of convergence G. We have proven the following statement: If f∈Ap(G) and h∈Hp, then for a given ε=(ε1,…,εp)∈R+p and arbitrary δ>0 there exists a set E⊂|G| such that ∫E∩Δεh(r)dr1⋯drpr1⋯rp<+∞ and for all r∈Δε∖E we have Mf(r)≤μf(r)(h(r))p+12lnp2+δh(r)lnp2+δ{μf(r)h(r)}∏j=1p(lnerjεj)p−12+δ. Note, that this assertion at p=1,G=C,h(r)≡const implies the classical Wiman–Valiron theorem for entire functions and at p=1, the G=D:={z∈C:|z|<1},h(r)≡1/(1−r) theorem about the Kővari-type inequality for analytic functions in the unit disc D; p>1 implies some Wiman’s type inequalities for analytic functions of several variables in Cn×Dk, n,k∈Z+,n+k∈N.
Temperature-Induced Precipitation of V2O5 in Vanadium Flow Batteries—Revisited
Authors: Kirk Emil Holm , Fenini Filippo , Oreiro Sara Noriega , Bentien Anders
Published: December 2021

The maximum operation temperature of the vanadium solution in vanadium flow batteries is typically limited to 40 °C to prevent the damaging thermal precipitation of V2O5. Therefore, the operation of batteries at high ambient temperatures is an important aspect to tackle for stationary storage. In the present work, a comprehensive study of the high temperature stability of redox solutions for vanadium flow batteries was performed. In particular, focus was placed on a comparison between batch and in operando precipitation experiments. It was found that, despite being a widely used method in the literature, caution should be taken when assessing the precipitation through capacity fade due to the large influence of external oxidation and cycling parameters, plausibly leading to an incorrect interpretation of the results. The in operando experiments consistently show a precipitation temperature almost 10–20 °C higher than in the batch tests at a 100% state of charge for the same time lapse.
The Battery Life Estimation of a Battery under Different Stress Conditions
Authors: Andrenacci Natascia , Vellucci Francesco , Sglavo Vincenzo
Published: December 2021

The prediction of capacity degradation, and more generally of the behaviors related to battery aging, is useful in the design and use phases of a battery to help improve the efficiency and reliability of energy systems. In this paper, a stochastic model for the prediction of battery cell degradation is presented. The proposed model takes its cue from an approach based on Markov chains, although it is not comparable to a Markov process, as the transition probabilities vary with the number of cycles that the cell has performed. The proposed model can reproduce the abrupt decrease in the capacity that occurs near the end of life condition (80% of the nominal value of the capacity) for the cells analyzed. Furthermore, we illustrate the ability of this model to predict the capacity trend for a lithium-ion cell with nickel manganese cobalt (NMC) at the cathode and graphite at the anode, subjected to a life cycle in which there are different aging factors, using the results obtained for cells subjected to single aging factors.
Assessing the Asynchrony Event Based on the Ventilation Mode for Mechanically Ventilated Patients in ICU
Authors: Muhamad Sauki Nur Sa’adah , Damanhuri Nor Salwa , Othman Nor Azlan , Chiew Meng Belinda Chong , Chiew Yeong Shiong , Mat Nor Mohd Basri
Published: December 2021

Respiratory system modelling can assist clinicians in making clinical decisions during mechanical ventilation (MV) management in intensive care. However, there are some cases where the MV patients produce asynchronous breathing (asynchrony events) due to the spontaneous breathing (SB) effort even though they are fully sedated. Currently, most of the developed models are only suitable for fully sedated patients, which means they cannot be implemented for patients who produce asynchrony in their breathing. This leads to an incorrect measurement of the actual underlying mechanics in these patients. As a result, there is a need to develop a model that can detect asynchrony in real-time and at the bedside throughout the ventilated days. This paper demonstrates the asynchronous event detection of MV patients in the ICU of a hospital by applying a developed extended time-varying elastance model. Data from 10 mechanically ventilated respiratory failure patients admitted at the International Islamic University Malaysia (IIUM) Hospital were collected. The results showed that the model-based technique precisely detected asynchrony events (AEs) throughout the ventilation days. The patients showed an increase in AEs during the ventilation period within the same ventilation mode. SIMV mode produced much higher asynchrony compared to SPONT mode (p < 0.05). The link between AEs and the lung elastance (AUC Edrs) was also investigated. It was found that when the AEs increased, the AUC Edrs decreased and vice versa based on the results obtained in this research. The information of AEs and AUC Edrs provides the true underlying lung mechanics of the MV patients. Hence, this model-based method is capable of detecting the AEs in fully sedated MV patients and providing information that can potentially guide clinicians in selecting the optimal ventilation mode of MV, allowing for precise monitoring of respiratory mechanics in MV patients.
Anthelmintic Activity and Cytotoxic Effects of Compounds Isolated from the Fruits of Ozoroa insignis Del. (Anacardiaceae)
Authors: Dube Mthandazo , Saoud Mohamad , Rennert Robert , Fotso Ghislain Wabo , Andrae-Marobela Kerstin , Imming Peter , Häberli Cécile , Keiser Jennifer , Arnold Norbert
Published: December 2021

Ozoroa insignis Del. is an ethnobotanical plant widely used in traditional medicine for various ailments, including schistosomiasis, tapeworm, and hookworm infections. From the so far not investigated fruits of Ozoroa insignis, the anthelmintic principles could be isolated through bioassay-guided isolation using Caenorhabditis elegans and identified by NMR spectroscopic analysis and mass spectrometric studies. Isolated 6-[8(Z)-pentadecenyl] anacardic (1), 6-[10(Z)-heptadecenyl] anacardic acid (2), and 3-[7(Z)-pentadecenyl] phenol (3) were evaluated against the 5 parasitic organisms Schistosoma mansoni (adult and newly transformed schistosomula), Strongyloides ratti, Heligmosomoides polygyrus, Necator americanus, and Ancylostoma ceylanicum, which mainly infect humans and other mammals. Compounds 1–3 showed good activity against Schistosoma mansoni, with compound 1 showing the best activity against newly transformed schistosomula with 50% activity at 1µM. The isolated compounds were also evaluated for their cytotoxic properties against PC-3 (human prostate adenocarcinoma) and HT-29 (human colorectal adenocarcinoma) cell lines, whereby compounds 2 and 3 showed antiproliferative activity in both cancer cell lines, while compound 1 exhibited antiproliferative activity only on PC-3 cells. With an IC50 value of 43.2 µM, compound 3 was found to be the most active of the 3 investigated compounds.
The Assignment of the Absolute Configuration of Non-Cyclic Sesquiterpenes by Vibrational and Electronic Circular Dichroism: The Example of Chiliadenus lopadusanus Metabolites
Authors: Mazzeo Giuseppe , Cimmino Alessio , Longhi Giovanna , Masi Marco , Evidente Antonio , Abbate Sergio
Published: December 2021

9-Hydroxynerolidol, 9-oxonerolidol, and chiliadenol B are three farnesane-type sesquiterpenoids isolated from Chiliadenus lopadusanus that have shown an interesting activity against human pathogens as Gram+ and Gram− bacteria resistant to antibiotics. However, the absolute configuration (AC) of these interesting sesquiterpenes has not been assigned so far. Vibrational and electronic circular dichroism spectra have been recorded and correlations are pointed out for the three compounds. Density functional theory (DFT) calculations are used in conjunction with Mosher’s method of investigation to assign AC. Statistical analysis is considered to quantitatively define the choice of AC from VCD spectra.
The Cross Marks the Spot: The Emerging Role of JmjC Domain-Containing Proteins in Myeloid Malignancies
Authors: Staehle Hans Felix , Pahl Heike Luise , Jutzi Jonas Samuel
Published: December 2021

Histone methylation tightly regulates chromatin accessibility, transcription, proliferation, and cell differentiation, and its perturbation contributes to oncogenic reprogramming of cells. In particular, many myeloid malignancies show evidence of epigenetic dysregulation. Jumonji C (JmjC) domain-containing proteins comprise a large and diverse group of histone demethylases (KDMs), which remove methyl groups from lysines in histone tails and other proteins. Cumulating evidence suggests an emerging role for these demethylases in myeloid malignancies, rendering them attractive targets for drug interventions. In this review, we summarize the known functions of Jumonji C (JmjC) domain-containing proteins in myeloid malignancies. We highlight challenges in understanding the context-dependent mechanisms of these proteins and explore potential future pharmacological targeting.
From Action to Cognition: Neural Reuse, Network Theory and the Emergence of Higher Cognitive Functions
Authors: Ptak Radek , Doganci Naz , Bourgeois Alexia
Published: December 2021

The aim of this article is to discuss the logic and assumptions behind the concept of neural reuse, to explore its biological advantages and to discuss the implications for the cognition of a brain that reuses existing circuits and resources. We first address the requirements that must be fulfilled for neural reuse to be a biologically plausible mechanism. Neural reuse theories generally take a developmental approach and model the brain as a dynamic system composed of highly flexible neural networks. They often argue against domain-specificity and for a distributed, embodied representation of knowledge, which sets them apart from modular theories of mental processes. We provide an example of reuse by proposing how a phylogenetically more modern mental capacity (mental rotation) may appear through the reuse and recombination of existing resources from an older capacity (motor planning). We conclude by putting arguments into context regarding functional modularity, embodied representation, and the current ontology of mental processes.
Rotation-Free Based Numerical Model for Nonlinear Analysis of Thin Shells
Authors: Smoljanović Hrvoje , Balić Ivan , Munjiza Ante , Hristovski Viktor
Published: December 2021

This paper presents a computationally efficient numerical model for the analysis of thin shells based on rotation-free triangular finite elements. The geometry of the structure in the vicinity of the observed triangular element is approximated through a controlled domain consisting of nodes of the observed finite element and nodes of three adjacent finite elements between which a second-order spatial polynomial is defined. The model considers large displacements, large rotations, small strains, and material and geometrical nonlinearity. Material nonlinearity is implemented by considering the von Mises yield criterion and the Levi–Mises flow rule. The model uses an explicit time integration scheme to integrate motion equations but an implicit radial returning algorithm to compute the plastic strain at the end of each time step. The presented numerical model has been embedded in the program Y based on the finite–discrete element method and tested on simple examples. The advantage of the presented numerical model is displayed through a series of analyses where the obtained results are compared with other results presented in the literature.
Assessing the Duration of the Lead Appointed Party Coordination Tasks and Evaluating the Appropriate Team Composition on BIM Projects
Authors: Mayer Pavol , Funtík Tomáš , Erdélyi Ján , Honti Richard , Cerovšek Tomo
Published: December 2021

This paper addresses critical success factors for the delivery of BIM projects. The lack of experience with BIM projects on both the demand and supply side often leads to insufficient project teams, unsatisfied clients, schedule, and cost overruns. In order to better structure and control the information delivery in BIM projects requirements, planning and delivery must be standardized. The latter was achieved by EIR (Exchange Information Requirements), new BIM roles, BEP (BIM Execution Plan), and specified digital handover, which must be supported by a common data environment (CDE). This paper provides an analysis of the characteristics of BIM project delivery and duration in Architectural and Engineering companies in Slovakia. The analysis is based on the web survey of BIM managers and coordinators, which reveals that a significant amount of BIM project efforts must be executed by BIM specialists. The results also graphically depict the scope of critical BIM activities across project phases. The presented study is relevant for various project stakeholders and allows for a deeper understanding of the resources needed for the successful delivery of BIM projects in terms of adequate project team capacity, capability, organization, and planning.
Physics-Based Ground Motion Simulations for the Prediction of the Seismic Vulnerability of Masonry Building Compounds in Mirandola (Italy)
Authors: Chieffo Nicola , Fasan Marco , Romanelli Fabio , Formisano Antonio , Mochi Giovanni
Published: December 2021

The current paper aims at investigating the seismic capacity of a masonry building aggregate in the historical centre of Mirandola based on a reliable ground motion simulation procedure. The examined clustered building is composed of eleven structural units (SUs) mutually interconnected to each other, which are made of brick walls and are characterized by wooden floors poorly connected to the vertical structures. Non-linear static analyses are performed by adopting the 3Muri software to characterize the seismic capacity of both the entire aggregate and the individual SUs. In this framework, a multi-scenario physics-based approach is considered for the definition of the seismic input in terms of broadband seismic signals inclusive of source and site effects. Finally, the incidence of the seismic input variability is discussed for the prediction of the global capacity response of the case study building.