bluuebunny/crossref_metadata_2025 huggingface dataset:
Automatic Segmentation of the Jaws Used in Guided Insertion of Orthodontic Mini Implants to Improve Their Stability and Precision
Authors: David Andra Patricia , Brad Silviu , Rusu Laura-Cristina , David Ovidiu Tiberiu , Samoila Christian , Leretter Marius Traian
Published: October 2024

Background and Objectives: With the goal of identifying regions with bicortical bone and avoiding root contact, the present study proposes an innovative technique for the simulation of the insertion of mini orthodontic implants using automatic jaw segmentation. The simulation of mini implants takes place in 3D rendering visualization instead of Multi-Planar Reconstruction (MPR) sections. Materials and Methods: The procedure involves utilizing software that automatically segments the jaw, teeth, and implants, ensuring their visibility in 3D rendering images. These segmented files are utilized as study models to determine the optimum location for simulating orthodontic implants, in particular locations characterized by limited distances between the implant and the roots, as well as locations where the bicortical structures are present. Results: By using this method, we were able to simulate the insertion of mini implants in the maxilla by applying two cumulative requirements: the implant tip needs to be positioned in a bicortical area, and it needs to be situated more than 0.6 mm away from the neighboring teeth’s roots along all of their axes. Additionally, it is possible to replicate the positioning of the mini implant in order to distalize the molars in the mandible while avoiding the mandibular canal and the path of molar migration. Conclusions: The utilization of automated segmentation and visualization techniques in 3D rendering enhances safety measures during the simulation and insertion of orthodontic mini implants, increasing the insertion precision and providing an advantage in the identification of bicortical structures, increasing their stability.
Exploring the Father’s Role in Determining Neonatal Birth Weight: A Narrative Review
Authors: Libretti Alessandro , Savasta Federica , Nicosia Anthony , Corsini Christian , De Pedrini Alberto , Leo Livio , Laganà Antonio Simone , Troìa Libera , Dellino Miriam , Tinelli Raffaele , Sorrentino Felice , Remorgida Valentino
Published: October 2024

Birth weight, which exhibits variability across different populations, is influenced by a mix of genetic, environmental, and dietary factors originating from both the mother and father. Maternal characteristics, including age, socioeconomic status, prior pregnancies, weight, height, and weight increase throughout pregnancy, have a substantial influence on fetal growth and the health of the infant. On the other hand, the influence of paternal characteristics on the weight of newborns is still not fully comprehended in a consistent manner. Birth weight is an important factor that can help predict various maternal complications, such as the probability of having a C-section, experiencing postpartum hemorrhage or infections. It can also indicate future health challenges like asthma, cognitive impairment, and chronic diseases such as hypertension and diabetes. Nineteen publications were found through a thorough search of the Medline, PubMed, and Scopus databases, which provide insights into how paternal variables contribute to variations in birth weight. Significantly, the age of the father was found to be associated with higher chances of preterm birth and having a smaller size for gestational age in premature infants, while full-term children were more likely to have a larger size for gestational age. In addition, there is a constant correlation between the height of the father and the birth weight of the child. Taller dads are more likely to have babies with a higher birth weight and a lower likelihood of being small for gestational age (SGA). Although there were some discrepancies in the data about the weight and BMI of fathers, it was found that the height of fathers played a significant role in determining the size of the fetus and the weight of the newborn. While there may be differences in the conducted studies, these findings provide valuable insights into the complex connection between parental characteristics and fetal development. This data can be utilized to enhance clinical treatment strategies and enhance our comprehension of outcomes for neonates. Further homogeneous investigations are required to conclusively validate and build upon these findings.
Breast Reconstruction in Patients with Prior Breast Augmentation: Searching for the Optimal Reconstructive Option
Authors: Tedeschi Pasquale , Elia Rossella , Gurrado Angela , Nacchiero Eleonora , Angelelli Alessia , Testini Mario , Giudice Giuseppe , Maruccia Michele
Published: October 2024

Background and Objectives: Breast cancer in patients with prior breast augmentation poses unique challenges for detection, diagnosis, and management. Mastectomy rates are increasing, and patients with prior augmentation often have a lower body mass index, making autologous techniques unsuitable. This study aims to assess the best reconstructive option in patients with a history of subglandular or dual-plane breast augmentation. Materials and methods: A prospective analysis was conducted on patients who underwent breast reconstruction after mastectomy. Patients with subglandular or dual-plane breast augmentation were included. Patients were divided into submuscular breast reconstruction (Group 2) or prepectoral breast reconstruction (Group 1) groups. Demographic and surgical data were collected. Results: A total of 47 patients were included, with 23 in Group 1 and 24 in Group 2. Complications occurred in 11 patients (23.4%), with significant differences between groups. The most common complication was seroma formation. Implant loss occurred in 4.3% of cases in Group 1, while no implant loss was observed in Group 2. Patient-reported satisfaction scores were similar between groups at 12 months postoperatively. Conclusions: Subpectoral breast reconstruction with a tissue expander seems a safer and effective technique for patients with prior breast augmentation. It resulted in fewer complications. This approach should be considered as an option for breast reconstruction after mastectomy in this cohort of patients.
Corrosion Behaviour of Heat-Treated Cold Spray Nickel Chromium/Chromium Carbides
Authors: Tan Cedric , Krishnan Kannoorpatti , Elumalai Naveen Kumar
Published: October 2024

Chromium carbide powder agglomerated with nickel/chrome was deposited using a cold spray process onto a mild steel substrate. The deposits were heat-treated at 650 °C and 950 °C in ambient conditions to reduce porosity and improve adhesion between powder particles. The corrosion behaviour of these cold-sprayed materials was studied in artificial seawater conditions using electrochemical techniques. Heat treatment at 650 °C was found to best improve corrosion resistance, while the 950 °C treatment performed better than the as-sprayed condition but lower than the 650 °C sample. Microstructural analysis revealed complex phase transformations and structural refinements with increasing heat treatment temperature. The crystallite size of both Cr3C2 and NiCr phases decreased, while microstrain and dislocation density increased due to heat treatment. The formation of and subsequent reduction in Cr23C6 content indicated a complex sequence of carbide dissolution, transformation, and precipitation processes. The 650 °C heat-treated sample demonstrated superior corrosion resistance, evidenced by the highest corrosion potential, lowest passive current, and largest charge transfer resistance. This enhanced performance was attributed to the formation of a more stable and protective passive film, optimal carbide dissolution, and a homogeneous microstructure. Meanwhile, the 950 °C treatment led to excessive carbide dissolution and formed increased interfaces between the carbide and matrix. Mechanical property changes were also observed, with carbide hardness significantly decreasing after corrosion testing. These findings highlight the critical role of controlled heat treatment in optimising the performance of cold-sprayed Cr3C2-NiCr coatings, demonstrating that achieving superior corrosion resistance requires a delicate balance between microstructural refinement, phase transformations, and preservation of coating integrity.
Plantar Load System Analysis Using FSR Sensors and Interpolation Methods
Authors: Trujillo-Hernández Gabriel , Ortiz-Villaseñor Dayanna , Rodríguez-Quiñonez Julio C. , Ramírez-Hernández Luis Roberto , Murrieta-Rico Fabian N. , Mercado-Herrera Abelardo , Raygoza-Limón María E. , Orduño-Osuna Jesús Heriberto
Published: October 2024

The foot is considered a wonder of biological engineering due to its structure, formed by bones, ligaments, and tendons that collaborate to ensure stability and mobility. A key area often examined by medical professionals in patients with diabetic feet is the plantar surface, due to the risk of ulcer development. If left untreated, these ulcers can lead to severe complications, including amputation of the toe, foot, or even the limb. Interpolation methods are used to find areas with overloads in a system of sensor maps that are based on capacitive, load cells, or force-sensitive resistors (FSRs). This manuscript presents the assessment of linear, nearest neighbors, and bicubic methods in comparison with ground truth to calculate the root mean square error (RMSE) in two assessments using a dataset of eight healthy subjects, four men and four women, with an average age of 25 years, height of 1.63 m, and weight of 72 kg with shoe sizes from 7.3 USA using FSR map with 48 sensors. Additionally, this paper describes the conditioning circuit development to implement a plantar surface system that enables interpolating loads on the plantar surface. The proposed system’s results show that the first assessment indicates an RMSE of 0.089, 0.126, and 0.089 for linear, nearest neighbor, and bicubic methods, while the second assessment shows a mean RMSE for linear, nearest neighbor, and bicubic methods of 0.114, 0.159, and 0.112.
Characterization of Microplastics in Bees and Their Products in Urban and Rural Areas of the Sabana De Bogotá, Colombia
Authors: Gómez-Méndez Luis David , Robles-Camargo Jorge E. , Vera-Bravo Ricardo , Moncaleano-Niño Angela M. , Devia Castillo Carlos A. , Ospina-Torres Rodulfo , Escobar-Cortés Yisela , Camacho-Erazo Mariana , Amarillo-Suárez Angela R.
Published: October 2024

Microplastics, plastic fragments smaller than 5 mm present in the environment due to the decomposition of larger plastics, can cause damage to various ecosystems and species of pollinating insects, such as Apis mellifera bees. These bees play a crucial role in the ecology and production of honey and pollen, also serving as bioindicators of environmental quality as they are sensitive to contaminants such as microplastics. In this study, we evaluated the presence of microplastics in these insects and their products—pollen, and honey—collected in August 2021 and August 2023 in rural areas (Tabio and Guasca) of Cundinamarca, Colombia, and urban areas (Universidad Nacional de Colombia and Pontificia Universidad Javeriana) of Bogotá, Colombia. Each year, 24 bees, 10 g of honey, and 5 g of pollen were collected per sampling point. Microplastics in bees and their products were identified and quantified by stereomicroscopy, with or without hydrogen peroxide digestion pretreatment. Microplastics were found in bees, pollen, and honey in both periods, with an increase in their quantity observed over time due to increasing environmental pollution. Blue fibers were the most common microplastics, with a greater amount recorded in 2023 compared to 2021.
Inferring the Orientations of the Foliation and Lineation Defined by Quartz c-Axis Fabrics: Methods and Applications
Authors: Takeshita Toru
Published: October 2024

In this manuscript, I introduce a convenient method to convert the Euler angles (complete crystal orientations) obtained with EBSD (electron back-scattered diffraction) to the azimuth and inclination angle of crystallographic axes to reconstruct the pole figures. This method, which is subsequently coupled with the rotation of the pole figures, is particularly useful for the analyses of quartz c-axis fabrics in the deformed rocks, where the foliation and/or lineation is not clear or unknown. Although we arbitrarily choose the sample coordinates (Xs-Ys-Zs) in such cases, it is often possible that we can rotate the quartz c-axis pole figures so that they will exhibit a well-defined intrinsic symmetry in the rotated sample frame. The rotated XsYs-plane and Xs-direction can be now called the foliation and lineation, respectively, inversely defined by the quartz c-axis fabrics. On the other hand, the foliation and lineation clearly defined by the shape-preferred orientations (SPOs) of platy or columnar minerals can be oblique to those defined by the quartz c-axis fabrics. In this case, the former foliation and lineation could represent the total strain, while the latter ones could represent the last incremental strain, indicating triclinic deformation symmetry (e.g., triclinic transpression).
Hybrid Modeling and Simulation of the Grinding and Classification Process Driven by Multi-Source Compensation
Authors: Yang Jiawei , Zou Guobin , Zhou Junwu , Wang Qingkai , Song Tao , Li Kang
Published: October 2024

The grinding process is a key link in mineral processing production and a typical complex, controlled process. The steady-state model is limited by its model structure and thus difficult to applyied in a control system. A hybrid modeling method driven by multi-source compensation is proposed in this paper based on the mechanism model using key equipment in the grinding and classification process, addressing the uncertainties which affect the stability of the control systems. This method combines the relevant multi-source signals with uncertainties by using a priori knowledge, extracts the nonlinear feature vector in the signal through an unsupervised depth network, and constructs a compensation model based on dynamic radial basis function network to realize the integration of mechanism modeling and data-driven compensation modeling. The simulation results show that the model has a high degree of fit to the real physical system; the industrial validation was conducted at a gold concentrator, the grinding product quality was predicted and controlled with the model.
Dissolution Kinetics of Carbonates in Low-Grade Microgranular Phosphate Ore Using Organic Acids as Leaching Agents
Authors: Raiymbekov Yerkebulan , Abdurazova Perizat , Nazarbek Ulzhalgas
Published: October 2024

The present study investigates the process of selective leaching of low-grade phosphate ore of the Karatau basin using organic acids such as formic and citric acids. Chemical and instrumental analyses of the investigated phosphate ore were carried out, including X-ray diffraction (XRD), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX), and Fourier-transform infrared spectroscopy (FTIR). Based on experimental data, reaction rate constants were calculated, and the obtained activation energies for each of the reagents were used. The reaction rate constants indicate that formic acid led to a more gradual increase in P2O5 concentration over time, while citric acid demonstrates a more significant increase in phosphorus concentration at all temperatures, especially at 70 °C. The activation energy for formic acid is 14.69 kJ/mol, indicating a diffusion-controlled reaction mechanism, whereas the activation energy for citric acid is higher, i.e., 35.78 kJ/mol, suggesting a more complex mechanism involving both diffusion and chemical processes. The present study highlights the importance of selecting appropriate reaction conditions to achieve maximum efficiency for the leaching of phosphate ore, depending on both temperature and reagent used.
Modeling of a Fluid with Pressure-Dependent Viscosity in Hele-Shaw Flow
Authors: Calusi Benedetta , Palade Liviu Iulian
Published: October 2024

We investigate the Hele-Shaw flow of fluids whose viscosity depends on pressure, i.e., piezo-viscous fluids, near the tip of a sharp edge. In particular, we consider both cases of two-dimensional symmetric and antisymmetric flows. To obtain the pressure field, we provide a procedure that is based on the method of separation of variables and does not depend on a specific choice of the expression for the pressure-dependent viscosity. Therefore, we show the existence of a general procedure to investigate the behavior of piezo-viscous fluids in Hele-Shaw flow and its solution near a sharp corner. The results are applied to the case of an exponential dependence of viscosity on pressure as an example of exact solutions for the pressure field.
Development of a UHPLC-MS/MS Method for the Determination of Moxidectin in Rat Plasma and Its Application in Pharmacokinetics
Authors: Zhang Hongjuan , Yang Zhen , Hao Baocheng , Wu Di , Shao Dan , Liu Yu , Pu Wanxia , Yi Shouli , Shang Ruofeng , Wang Shengyi
Published: October 2024

The aim of the present study was to establish a simple and reliable ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method and apply it for the determination of pharmacokinetics of moxidectin-loaded microspheres (MOX-MS) in rats. Plasma samples were processed using a simplified liquid–liquid extraction method and were separated using an Agilent Zorbax Eclipse Plus C18 column (50 mm × 2.1 mm, 1.8 μm) with a mobile phase consisting of a 10 mM ammonium formate solution with 0.1% formic acid (A) and acetonitrile (B) at a flow rate of 0.4 mL/min for 5 min. Avermectin B1a was used as an internal standard (IS). The sample was injected at a volume of 10 μL with a column temperature of 35 °C and detected in a positive ion mode. A good linear response across the concentration range of 1.00–200 ng/mL (r2 > 0.99) and a lower limit of quantification (LLOQ) of 1.00 ng/mL were achieved. The extraction recovery of moxidectin exceeded 94.1%, the matrix effect was between 91.2% and 96.2%, the accuracy ranged from 100.1 to 103.6%, and the relative standard deviation (RSD) did not exceed 15% for the intra- and inter-day accuracy and precision. The pharmacokinetic results showed that MOX-MS significantly decreased Cmax, prolonged T1/2, and improved bioavailability. The developed method significantly reduced the assay volume, shortened detection time, simplified sample processing methods and saved assay costs, which may contribute to the development of the new antiparasitic drug.
Carbon Dots and Their Films with Narrow Full Width at Half Maximum Orange Emission
Authors: Wu Jiangchen , Liu Jianan , Liu Xinghua , Zheng Jingxia , Chen Lin , Yang Yongzhen , Su Chunhui
Published: October 2024

To obtain carbon dots (CDs) with narrow full width at half maximum (FWHM) and long-wavelength emission, carbon sources with high conjugate sizes and abundant functional groups can be employed to synthesize CDs. In this study, orange-emissive carbon dots (OCDs) were synthesized with phloroglucinol and rhodamine B as precursors. When the molar ratio of them was 30:1, and ethanol was served as the solvent, OCDs with optimized emission wavelength at approximately 580 nm, an FWHM of 30 nm, and a quantum yield (QY) of 27.31% were obtained. Subsequently, the OCDs were incorporated into polyvinyl alcohol (PVA) to fabricate solid-state OCD/PVA fluorescent films, which exhibited an FWHM of 47 nm. The PVA matrix facilitated the dispersion of OCDs, thereby suppressing non-radiative energy transfer among the OCDs and enhancing luminescence efficiency. Consequently, compared with OCDs, the OCD/PVA film exhibited significant luminescent enhancement, and the QY of the composite film was increased to 84.74%. Moreover, OCD/PVA film showed good transmittance and thermal stability. This research offers a solid theoretical and experimental foundation for the potential applications of CDs in the field of solid-state lighting.
Electron Density and Molecular Orbital Analyses of the Nature of Bonding in the η3-CCH Agostic Rhodium Complexes Preceding the C–C and C–H Bond Cleavages
Authors: Efremenko Irena
Published: October 2024

In our recent work, we revisited C–H and C–C bond activation in rhodium (I) complexes of pincer ligands PCP, PCN, PCO, POCOP, and SCS. Our findings indicated that an η3-Csp2Csp3H agostic intermediate acts as a common precursor to both C–C and C–H bond activation in these systems. We explore the electronic structure and bonding nature of these precleavage complexes using electron density and molecular orbital analyses. Using NBO, IBO, and ESI-3D methods, the bonding in the η3-CCH agostic moiety is depicted by two three-center agostic bonds: Rh–Csp2–Csp3 and Rh–Csp3–H, with all three atoms datively bound to Rh(I). IBO analysis specifically highlights the involvement of three orbitals (CC→Rh and CH→Rh σ donation, plus Rh→CCH π backdonation) in both C–C and C–H bond cleavages. NCIPLOT and QTAIM analyses highlight anagostic (Rh–H) or β-agostic (Rh–Csp2–H) interactions and the absence of Rh–Csp3 interactions. QTAIM molecular graphs suggest bond path instability under dynamic conditions due to the nearness of line and ring critical points. Several low-frequency and low-force vibrational modes interconvert various bonding patterns, reinforcing the dynamic η3-CCH agostic nature. The kinetic preference for C–H bond breaking is attributed to the smaller reduced mass of C–H vibrations compared to C–C vibrations.
Rapid and Ultrasensitive Detection of H. aduncum via the RPA-CRISPR/Cas12a Platform
Authors: Wang Xiaoming , Chen Xiang , Xu Ting , Jin Xingsheng , Jiang Junfang , Guan Feng
Published: October 2024

Hysterothylacium aduncum is one of six pathogens responsible for human anisakiasis. Infection with H. aduncum can cause acute abdominal symptoms and allergic reactions and is prone to misdiagnosis in clinical practice. This study aims to enhance the efficiency and accuracy of detecting H. aduncum in food ingredients. We targeted the internal transcribed spacer 1 (ITS 1) regions of Anisakis to develop a visual screening method for detecting H. aduncum using recombinase polymerase amplification (RPA) combined with the CRISPR/Cas12a system. By comparing the ITS 1 region sequences of eight nematode species, we designed specific primers and CRISPR RNA (crRNA). The specificity of RPA primers was screened and evaluated, and the CRISPR system was optimized. We assessed its specificity and sensitivity and performed testing on commercial samples. The results indicated that the alternative primer ADU 1 was the most effective. The final optimized concentrations were 250 nM for Cas12a, 500 nM for crRNA, and 500 nM for ssDNA. The complete test procedure was achievable within 45 min at 37 °C, with a limit of detection (LOD) of 1.27 pg/μL. The amplified product could be directly observed using a fluorescence microscope or ultraviolet lamp. Detection results for 15 Anisakis samples were entirely consistent with those obtained via Sanger sequencing, demonstrating the higher efficacy of this method for detecting and identifying H. aduncum. This visual detection method, characterized by simple operation, visual results, high sensitivity, and specificity, meets the requirements for food safety testing and enhances monitoring efficiency.
Hydrodynamic Cavitation as a Method of Removing Surfactants from Real Carwash Wastewater
Authors: Lebiocka Magdalena , Montusiewicz Agnieszka , Grządka Elżbieta , Pasieczna-Patkowska Sylwia , Montusiewicz Jerzy , Szaja Aleksandra
Published: October 2024

The present work aimed to evaluate whether the use of an innovative method such as hydrodynamic cavitation (HC) is suitable for the simultaneous removal of surfactants of different chemical natures (non-ionic, anionic and cationic) from actual car wash wastewater at different numbers of passes through the cavitation zone and different inlet pressures. An additional novelty was the use of multi-criteria decision support, which enabled the selection of optimal HC conditions that maximized the removal of each group of surfactants and chemical oxygen demand (COD) with minimal energy input. For the optimal HC variants, Fourier transform infrared spectroscopy (FT-IR/ATR) as well as investigations of surface tension, zeta potential, specific conductivity, system viscosity and particle size were carried out. The highest reduction of non-ionic surfactants was found at 5 bar inlet pressure and reached 35.5% after 120 min. The most favourable inlet pressure for the removal of anionic surfactants was 3 bar and the removal efficiency was 77.2% after 120 min, whereas the most favourable inlet pressure for cationic surfactant removal was 3 bar, with the highest removal of 20% after 120 min. The obtained results clearly demonstrate that HC may constitute an effective, fast and cost-efficient method for removing surfactants from real industrial wastewater.
SARS-CoV-2 Nucleocapsid Protein Antagonizes GADD34-Mediated Innate Immune Pathway through Atypical Foci
Authors: Liu Jie , Guan Guanwen , Wu Chunxiu , Wang Bingbing , Chu Kaifei , Zhang Xu , He Su , Zhang Naru , Yang Geng , Jin Zhigang , Zhao Tiejun
Published: October 2024

The integrated stress response, especially stress granules (SGs), contributes to host immunity. Typical G3BP1+ stress granules (tSGs) are usually formed after virus infection to restrain viral replication and stimulate innate immunity. Recently, several SG-like foci or atypical SGs (aSGs) with proviral function have been found during viral infection. We have shown that the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein induces atypical N+/G3BP1+ foci (N+foci), leading to the inhibition of host immunity and facilitation of viral infection. However, the precise mechanism has not been well clarified yet. In this study, we showed that the SARS-CoV-2 N (SARS2-N) protein inhibits dsRNA-induced growth arrest and DNA damage-inducible 34 (GADD34) expression. Mechanistically, the SARS2-N protein promotes the interaction between GADD34 mRNA and G3BP1, sequestering GADD34 mRNA into the N+foci. Importantly, we found that GADD34 participates in IRF3 nuclear translocation through its KVRF motif and promotes the transcription of downstream interferon genes. The suppression of GADD34 expression by the SARS2-N protein impairs the nuclear localization of IRF3 and compromises the host’s innate immune response, which facilitates viral replication. Taking these findings together, our study revealed a novel mechanism by which the SARS2-N protein antagonized the GADD34-mediated innate immune pathway via induction of N+foci. We think this is a critical strategy for viral pathogenesis and has potential therapeutic implications.
MUF-n-Octadecane Phase-Change Microcapsules: Effects of Core pH and Core–Wall Ratio on Morphology and Thermal Properties of Microcapsules
Authors: Lin Lin , Li Ziqi , Zhang Jian , Ma Tonghua , Wei Renzhong , Zhang Qiang , Shi Junyou
Published: October 2024

Phase change energy storage microcapsules were synthesized in situ by using melamine-formaldehyde–urea co-condensation resin (MUF) as wall material, n-octadecane (C18) as core material and styryl-maleic anhydride copolymer (SMA) as emulsifier. Fourier transform infrared spectroscopy, scanning electron microscopy, differential scanning calorimetry and thermogravimetric analysis were used to study the effects of emulsifier type, emulsifier dosage, core–wall ratio and pH on the morphology and thermal properties of microcapsules. The results show that the pH of core material and the ratio of core to wall have a great influence on the performance of microcapsules. SMA emulsifiers and MUF are suitable for the encapsulation of C18. When the pH is 4.5 and the core–wall ratio is 2/1, the latent heat and encapsulation efficiency of phase transition reaches 207.3 J g−1 and 84.7%, respectively. The prepared phase-change microcapsules also have good shape stability and thermal stability.
Study on the Influence of Thermoplastic Microcapsules on the Sulfate Resistance and Self-Healing Performance of Limestone Calcined Clay Cement Concrete
Authors: Du Wei , Jiang Lu , Liu Quantao , Chen Wei , Ding Qingjun
Published: October 2024

Limestone calcined clay cement (LC3), enhanced through reactions with volcanic ash and the interaction between limestone and clay, significantly improves the performance of cementitious materials. It has the potential to cut CO2 emissions by up to 30% and energy consumption in cement manufacture by 15% to 20%, providing a promising prospect for the large-scale production of low-carbon cement with a lower environmental effect. To effectively manufacture LC3 concrete, this study utilized limestone (15%), calcined clay (30%), and gypsum (5%) as supplementary cementitious materials (SCMs), replacing 50% of ordinary Portland cement (OPC). However, in regions abundant in sulfate, sulfate attack can cause interior cracking of concrete, reducing the longevity of the building. To address this issue, microcapsules containing microcrystalline wax, ceresine wax, and nano-CaCO3 encapsulated in epoxy resin were prepared and successfully incorporated into LC3 concrete. Sulfate resistance tests were conducted through sulfate dry–wet cycles, comparing samples with and without microcapsules. The findings revealed that the initial mechanical and permeability properties of LC3 concrete did not significantly differ from OPC concrete. LC3 concrete with added microcapsules (SP4) exhibited enhanced resistance to sulfate attack, reducing mass loss and compressive strength degradation. SEM images displayed a mesh-like structure of repair products in SP4. After 14 days of self-repair, SP4 exhibited a 44.2% harmful pore ratio, 98.1% compressive strength retention, 88.7% chloride ion diffusion coefficient retention, 91.12 mV maximum amplitude, and 9.14 mV maximum frequency amplitude. The experimental results indicate that the presence of microcapsules enhances the sulfate attack self-healing performance of LC3 concrete.
A Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to Quantify Wine Polyphenols
Authors: Flores Damien , Meudec Emmanuelle , Dias Aécio Luís de Sousa , Sommerer Nicolas
Published: October 2024

Polyphenols are responsible for wine colour and astringency, and, as antioxidants, they also have beneficial health properties. In this work, we developed a robust full-scan high-resolution mass spectrometry method for the quantification of 90 phenolic compounds in wine samples (either red, rosé, or white wine), using a UHPLC-OrbitrapTM system. With this method, we could conduct a detailed analysis of phenolic compounds in red, rosé, and white wines with great selectivity due to sub-ppm mass accuracy. Moreover, accessing the full-scan spectrum enabled us to monitor all the other compounds detected in the sample, facilitating the adaptability of this method to new phenolic compounds if needed.
Unsupervised Learning for the Automatic Counting of Grains in Nanocrystals and Image Segmentation at the Atomic Resolution
Authors: Sohn Woonbae , Kim Taekyung , Moon Cheon Woo , Shin Dongbin , Park Yeji , Jin Haneul , Baik Hionsuck
Published: October 2024

Identifying the grain distribution and grain boundaries of nanoparticles is important for predicting their properties. Experimental methods for identifying the crystallographic distribution, such as precession electron diffraction, are limited by their probe size. In this study, we developed an unsupervised learning method by applying a Gabor filter to HAADF-STEM images at the atomic level for image segmentation and automatic counting of grains in polycrystalline nanoparticles. The methodology comprises a Gabor filter for feature extraction, non-negative matrix factorization for dimension reduction, and K-means clustering. We set the threshold distance and angle between the clusters required for the number of clusters to converge so as to automatically determine the optimal number of grains. This approach can shed new light on the nature of polycrystalline nanoparticles and their structure–property relationships.
Palladium-Functionalized Nanostructured Nickel–Cobalt Oxide as Alternative Catalyst for Hydrogen Sensing Using Pellistors
Authors: Yurchenko Olena , Benkendorf Mike , Diehle Patrick , Schmitt Katrin , Wöllenstein Jürgen
Published: October 2024

To meet today’s requirements, new active catalysts with reduced noble metal content are needed for hydrogen sensing. A palladium-functionalized nanostructured Ni0.5Co2.5O4 catalyst with a total Pd content of 4.2 wt% was synthesized by coprecipitation to obtain catalysts with an advantageous sheet-like morphology and surface defects. Due to the synthesis method and the reducible nature of Ni0.5Co2.5O4 enabling strong metal-metal oxide interactions, the palladium was highly distributed over the metal oxide surface, as determined using scanning transmission electron microscopy and energy-dispersive X-ray investigations. The catalyst tested in planar pellistor sensors showed high sensitivity to hydrogen in the concentration range below the lower flammability limit (LFL). At 400 °C and in dry air, a sensor response of 109 mV/10,000 ppm hydrogen (25% of LFL) was achieved. The sensor signal was 4.6-times higher than the signal of pristine Ni0.5Co2.5O4 (24.6 mV/10,000 ppm). Under humid conditions, the sensor responses were reduced by ~10% for Pd-functionalized Ni0.5Co2.5O4 and by ~27% for Ni0.5Co2.5O4. The different cross-sensitivities of both catalysts to water are attributed to different activation mechanisms of hydrogen. The combination of high sensor sensitivity to hydrogen and high signal stability over time, as well as low cross-sensitivity to humidity, make the catalyst promising for further development steps.
Eco-Friendly Facile Conversion of Waste Eggshells into CaO Nanoparticles for Environmental Applications
Authors: Adaikalam Kathalingam , Hussain Sajjad , Anbu Periasamy , Rajaram Arulmozhi , Sivanesan Iyyakkannu , Kim Hyun-Seok
Published: October 2024

Amongst the many types of food waste, eggshells contain various minerals and bioactive materials, and they can become hazardous if not properly disposed of. However, they can be made useful for the environment and people by being converted to environmentally friendly catalytic materials or environmental purification agents. Simple calcination can enhance their properties and thereby render them suitable for catalytic and environmental applications. This work aimed to prepare CaO from waste eggshells and examine its effectiveness in photocatalytic pollution remediation, electrocatalytic activity, optical sensing, and antibacterial activities. As opposed to other techniques, this calcination process does not require any chemical reagents due to the high purity of CaCO3 in eggshells. Calcium oxide nanoparticles were prepared by subjecting waste eggshells (ES) to high-temperature calcination, and the synthesized CaO nanoparticles were characterized for their structural, morphological, chemical, optical, and other properties. Furthermore, their photocatalytic degradation of methylene blue dye and antibacterial efficiency against Escherichia coli and Staphylococcus aureus were investigated. It was found that the green-converted CaO can be efficiently used in environmental applications, showing good catalytic properties.
Ultrastable and Low-Threshold Two-Photon-Pumped Amplified Spontaneous Emission from CsPbBr3/Ag Hybrid Microcavity
Authors: Li Shulei , Zhang Yatao , Zhao Zhiran , Cheng Shiyi , Li Zixin , Liu Yuanyuan , Deng Quantong , Dai Jun , Zheng Yunbao , Lin Zhenxu
Published: October 2024

Halide perovskite materials have garnered significant research attention due to their remarkable performance in both photoharvesting photovoltaics and photoemission applications. Recently, self-assembled CsPbBr3 superstructures (SSs) have been demonstrated to be promising lasing materials. In this study, we report the ultrastable two-photon-pumped amplified stimulated emission from a CsPbBr3 SS/Ag hybrid microcavity with a low threshold of 0.8 mJ/cm2 at room temperature. The experimental results combined with numerical simulations show that the CsPbBr3 SS exhibits a significant enhancement in the electromagnetic properties in the hybrid microcavity on Ag film, leading to the uniform spatial temperature distribution under the irradiation of a pulsed laser, which is conducive to facilitate the recrystallization process of the QDs and improve their structural integrity and optical properties. This study provides a new idea for the application of CsPbBr3/Ag hybrid microcavity in photonic devices, demonstrating its potential in efficient optical amplification and upconversion lasers.
Implementation of Green Lean Six Sigma in Dutch Healthcare: A Qualitative Study of Healthcare Professionals’ Experiences
Authors: Sijm-Eeken Marieke , Greif Annick , Peute Linda , Jaspers Monique
Published: October 2024

Introduction: The healthcare sector significantly contributes to environmental degradation, highlighting the need for sustainable practices. Green Lean Six Sigma (GLSS) offers a relevant and impactful approach to reduce healthcare’s environmental footprint while improving efficiency. By incorporating environmental considerations into Lean Six Sigma, GLSS has the potential to mitigate healthcare’s environmental impact and promote environmental sustainability. This study aims to gain insight into healthcare professionals’ experiences with GLSS at their workplace. Materials and Methods: This qualitative exploratory study employed semi-structured surveys based on theory of training evaluation from Kirkpatrick with Dutch healthcare professionals in the first six to eight months after completing GLSS training. Results: Even though 76% (N = 16) of trained healthcare professionals applied GLSS at their workplace and 43% of them (N = 9) completed a project within the first six to eight months after training, they all experienced one or more barriers. The most frequently reported barriers were lack of time, difficulties with project selection and a lack of management support. GLSS project results included reduction of products, energy, costs and travel, green choices in procurement and sustainable food choices. GLSS also helped to create awareness on the environmental impact of healthcare and to optimize processes by reducing costs, waiting time, workload and defects. Discussion: This is the first study to report experiences from applying GLSS in healthcare. Furthermore, it is the first study presenting GLSS training evaluation results in terms of participant behaviour and organizational outcomes. Conclusions: Results of this study can be used to enhance GLSS deployment programs and to optimize organizational settings for successful GLSS implementation in healthcare.
The Effects of Elevated Temperatures on the Reproductive Biology of a Mediterranean Coral, Oculina patagonica
Authors: Shemesh Tamar , Levy Shani , Einbinder Abigail , Kolsky Itai , Bellworthy Jessica , Mass Tali
Published: October 2024

Global climate change is profoundly impacting coral ecosystems. Rising sea surface temperatures, in particular, disrupt coral reproductive synchrony, cause bleaching, and mortality. Oculina patagonica, a temperate scleractinian coral abundant across the Mediterranean Sea, can grow at a temperature range of 10–31 °C. Studies conducted three decades ago documented this species bleaching during the summer months, the same time as its gonads mature. However, the Eastern Mediterranean Sea is experiencing some of the fastest-warming sea surface temperatures worldwide. This study repeated the year-round in situ assessment of the reproductive cycle and gonad development and correlation to summer bleaching. In addition, thermal performance of the holobiont was assessed in an ex situ thermal stress experiment. In situ monitoring revealed no temporal changes in gonad development compared to previous studies, despite sea surface warming and concurrent bleaching. Experimental thermal performance curves indicated that photosynthetic rate peaked at 23 °C, bleached coral area was significant at 29 °C, and peaked at 34 °C. With local sea surface temperature reaching 31 °C, O. patagonica is exposed beyond its bleaching threshold during the summer months in situ. Despite this, O. patagonica maintains gonad development and physiologically recovers at the end of summer demonstrating resilience to current warming trends.
Mandibular Advancement after Pubertal Peak with Acrylic Splint Herbst Appliance Anchored to Four Miniscrews: A Retrospective Controlled Study
Authors: Manni Antonio , Boggio Andrea , Castellana Fabio , Gastaldi Giorgio , Cozzani Mauro
Published: October 2024

Objectives: The treatment of class II patients with mandibular retrusion often involves the use of a Herbst appliance, due to its efficiency and reduced need for compliance. Despite skeletal benefits, undesired dental effects, caused by anchorage loss, could reduce mandibular advancement, especially after the pubertal peak. Although the period between cervical vertebral maturation (CVM) 3 and CMV4 is considered the best choice to maximize the orthopedic outcome, clinicians cannot always treat patients during this ideal time window, as they present at their first visit later. The goal of this study is to evaluate whether the combination of an acrylic splint Herbst appliance with skeletal anchorage and elastic chains in both the upper and lower arch (Skeletal Therapy Manni Telescopic Herbst 4: STM4) could improve the efficiency of functional treatment also in CVM 5–6 patients. Methods: Lateral cephalograms of 10 consecutively treated patients taken at the beginning and at the end of the Herbst phase were analyzed and compared with those of 10 untreated patients of the same sex, age, malocclusion, and skeletal maturation. Results: According to the Pancherz analysis, the treated group of patients showed the significant advancement of the pogonion (4.75 mm), with a reduction in ANB (−3.3°) and Wits (−4.15 mm). The combination of miniscrews and elastic ligatures allowed the labial tipping of the upper incisors (+6.65°) and limited the labial flaring of the lower ones (+3.05°), maintaining the overjet needed for mandibular advancement. Conclusions: The STM4 technique might be an effective protocol for skeletal class II correction after the pubertal peak since it significantly reduces unfavorable dental compensations, increasing the skeletal effects and the esthetic outcome of the orthopedic treatment.
Investigating Anthrax-Associated Virulence Genes among Archival and Contemporary Bacillus cereus Group Genomes
Authors: Sabin Susanna J. , Beesley Cari A. , Marston Chung K. , Paisie Taylor K. , Gulvik Christopher A. , Sprenger Gregory A. , Gee Jay E. , Traxler Rita M. , Bell Melissa E. , McQuiston John R. , Weiner Zachary P.
Published: October 2024

Bacillus anthracis causes anthrax through virulence factors encoded on two plasmids. However, non-B. anthracis organisms within the closely related, environmentally ubiquitous Bacillus cereus group (BCG) may cause an anthrax-like disease in humans through the partial adoption of anthrax-associated virulence genes, challenging the definition of anthrax disease. To elucidate these phenomena and their evolutionary past, we performed whole-genome sequencing on non-anthracis BCG isolates, including 93 archival (1967–2003) and 5 contemporary isolates (2019–2023). We produced annotated genomic assemblies and performed a pan-genome analysis to identify evidence of virulence gene homology and virulence gene acquisition by linear inheritance or horizontal gene transfer. At least one anthrax-associated virulence gene was annotated in ten isolates. Most homologous sequences in archival isolates showed evidence of pseudogenization and subsequent gene loss. The presence or absence of accessory genes, including anthrax-associated virulence genes, aligned with the phylogenetic structure of the BCG core genome. These findings support the hypothesis that anthrax-associated virulence genes were inherited from a common ancestor in the BCG and were retained or lost across different lineages, and contribute to a growing body of work informing public health strategies related to anthrax surveillance and identification.
Multiple Benefits of Empagliflozin in PCOS: Evidence from a Preclinical Rat Model
Authors: Rakic Dejana , Jakovljevic Vladimir , Zivkovic Vladimir , Jakovljevic Uzelac Jovana , Jovic Nikola , Muric Maja , Pindovic Bozidar , Dimitrijevic Aleksandra , Arsenijevic Petar , Rakic Jovan , Mitrovic Slobodanka , Vulovic Tatjana , Joksimovic Jovic Jovana
Published: October 2024

Polycystic ovary syndrome (PCOS) is the most common complex endocrinological condition of women that is associated with infertility and metabolic disorders during the reproductive period. Recently, a great deal of research has focused on the etiopathogenesis of this disorder and the modulation of therapeutic approaches. There are still many controversies in the choice of therapy, and metformin is one of the most commonly used agents in the treatment of PCOS. Considering the link between metabolic disorders and PCOS, glycemic status is crucial in these patients, and sodium-glucose cotransporter type 2 inhibitors (SGLT2is) represent a potentially promising new therapeutic approach. These drugs have been shown to improve glucose metabolism, reduce adipose tissue, decrease oxidative stress, and protect the cardiovascular system. These data prompted us to investigate the effects of empagliflozin (EMPA) in a PCOS rat model and compare them with the effects of metformin. We confirmed that EMPA positively affects somatometric parameters, glucose and lipid metabolism, and the levels of sex hormones, as well as reduces oxidative stress and improves ovarian function and morphology. Administration of EMPA at doses of 5 mg/kg, 15 mg/kg, and 45 mg/kg during a 4-week treatment period improved, as induced by estradiol valerate and a high-fat diet, the metabolic and reproductive statuses in a PCOS rat model. The best effects, which were comparable to the effects of metformin, were achieved in groups receiving the middle and highest applied doses of EMPA. These results may prompt further clinical research on the use of EMPA in patients with PCOS.