bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of consuming diets containing various fats or citrus flavanones on plasma lipid and urinary F2-isoprostane concentrations in overweight cats
Authors: Jeusette Isabelle , Torre Celina , Salas Anna , Iraculis Neus , Compagnucci Marco , Romano Victor , Kirschvink Nathalie
Published: September 2010

Objective—To compare in overweight cats the effects of feeding moderate-energy diets with moderate fat content but with saturated fat (beef tallow), saturated fat plus citrus flavanones, or monounsaturated fat (olive oil) on plasma lipids and urinary F2-isoprostane concentrations.

Animals—20 overweight cats with mean ± SD body weight of 5.2 ± 0.2 kg and mean body condition score of 7.8 ± 0.2 (9-point scale).

Procedures—Body weight, plasma total cholesterol and triacylglycerol concentrations, and urinary F2-isoprostane concentration (as marker of oxidative stress) were measured at the beginning of the study, when the cats were fed a maintenance diet, and after 1, 3, and 5 months of consuming test diets.

Results—In overweight cats, citrus flavanones supplementation of the saturated fat diet was associated with lower energy intake and with lower plasma lipids and urinary F2-isoprostane concentrations than in cats fed the saturated fat alone. Monounsaturated fat feeding resulted in lower food intake than in cats fed saturated fat. However, plasma lipids concentrations remained within reference limits throughout the study.

Conclusions and Clinical Relevance—Although the clinical relevance of these findings is unknown, the significant differences detected indicated that lower energy intake with citrus flavanones supplementation or with substitution of saturated fat for monounsaturated fat could be good strategies for decreasing plasma lipids concentration and oxidative stress in overweight cats, even before considerable loss of body weight is observed. (Am J Vet Res 2010;71:1039–1044)
Risk factors for the incidence of calcium oxalate uroliths or magnesium ammonium phosphate uroliths for dogs in Ontario, Canada, from 1998 to 2006
Authors: Wisener Lee V. , Pearl David L. , Houston Doreen M. , Reid-Smith Richard J. , Moore Andrew E.
Published: September 2010

Objective—To investigate individual- and community-level contextual variables as risk factors for submission of calcium oxalate (CaOx) uroliths or magnesium ammonium phosphate (ie, struvite) uroliths for dogs to a national urolith center, as determined on the basis of urolith submission patterns.

Sample Population—Records of 7,297 dogs from Ontario, Canada, with CaOx or struvite uroliths submitted to the Canadian Veterinary Urolith Centre from 1998 through 2006.

Procedures—Data were analyzed via multilevel multivariable logistic regression.

Results—Individual-level main effects and interactions significantly associated with the risk of submission of CaOx uroliths rather than struvite uroliths included age, sex, breed group, neuter status, body condition, dietary moisture content, diet type, sex-neuter status interaction, sex-age interaction, body condition-age interaction, and breed group—dietary moisture content interaction. In addition, median community family income and being located within a major urban center (ie, Toronto) were significant risk factors for submission of CaOx uroliths, compared with submission of struvite uroliths.

Conclusions and Clinical Relevance—Individual-level and dietary factors for dogs affected the risk of submission of CaOx uroliths, relative to that of struvite uroliths. Interactions among these variables need to be considered when assessing the impact of these risk factors. In addition, community-level or contextual factors (such as community family income and residing in a densely populated area of Ontario) also affected submission patterns, although most of the variance in the risk for submission of CaOx uroliths, compared with the risk for submission of struvite uroliths, was explained by individual-level factors. (Am J Vet Res 2010;71:1045–1054)
Images of Desire
Authors: Jones Carla
Published: September 2010

The recent and highly visible rise of Islamic consumer culture in contemporary urban Indonesia is a source of both pleasure and anxiety for many Indonesians, figuring in debates about the appeal of a new piety there in the past decade. At the center of these debates is the image of the piously dressed woman. Simultaneously a consumer and a sign of piety, modest yet attractive, she seems to blur assumptions about the boundaries between image and substance, and in so doing generates anxiety. A booming Islamic fashion industry and Islamic fashion media traffic in this space, turning virtue into value and vice versa by deploying the image of the pious feminine to incite consumer desire while denying accusations that this is simply capitalism with a religious face. Based on research and interviews with the editorial staff of one Islamic fashion magazine, NooR, this article traces how Indonesia’s rising Islamic fashion industry and lifestyle media have placed women at the center of broader cultural debates about the relationship between devotion and consumption.
Giant posterior fossa cavernous malformations in 2 infants with familial cerebral cavernomatosis: the case for early screening
Authors: Lew Sean M.
Published: September 2010

The author reports the details in 2 cases of infants with familial cerebral cavernomatosis who presented in dire condition from hemorrhagic posterior fossa cavernous malformations.

In Case 1, a 4-month-old boy presented with opisthotonos, gaze palsy, and lethargy. Magnetic resonance imaging revealed a multilobulated cavernous malformation in the fourth ventricle with evidence of bleeding and obstructive hydrocephalus. In Case 2, a 7-month-old girl presented with lethargy, followed by rapid neurological decline. Imaging demonstrated a large lesion involving both the brainstem and cerebellum, with obstructive hydrocephalus. Both patients required immediate surgical intervention, and external ventricular drainage and posterior fossa craniotomies were performed. Both patients made excellent recoveries. These cases suggest that infants in families with suspected or confirmed familial cerebral cavernomatosis should be screened at an early age.
Surgical approaches to brainstem cavernous malformations
Authors: Abla Adib A. , Turner Jay D. , Mitha Alim P. , Lekovic Gregory , Spetzler Robert F.
Published: September 2010

Brainstem cavernous malformations (CMs) are low-flow vascular lesions in eloquent locations. Their presentation is often marked with symptomatic hemorrhages that appear to occur more frequently than hemorrhage from supratentorial cavernomas. Brainstem CMs can be removed using 1 of the 5 standard skull-base approaches: retrosigmoid, suboccipital (with or without telovelar approach), supracerebellar infratentorial, orbitozygomatic, and far lateral.

Patients being referred to a tertiary institution often have lesions that are aggressive with respect to bleeding rates. Nonetheless, the indications for surgery, in the authors' opinion, are the same for all lesions: those that are symptomatic, those that cause mass effect, or those that abut a pial surface. Patients often have relapsing and remitting courses of symptoms, with each hemorrhage causing a progressive and stepwise decline. Many patients experience new postoperative deficits, most of which are transient and resolve fully. Despite the risks associated with operating in this highly eloquent tissue, most patients have had favorable outcomes in the authors' experience. Surgical treatment of brainstem CMs protects patients from the potentially devastating effects of rehemorrhage, and the authors believe that the benefits of intervention outweigh the risks in patients with the appropriate indications.
The pathogenetic features of cerebral cavernous malformations: a comprehensive review with therapeutic implications
Authors: Krisht Khaled M. , Whitehead Kevin J. , Niazi Toba , Couldwell William T.
Published: September 2010

Cerebral cavernous malformations (CCMs) are common vascular lesions of the CNS that may lead to seizures, focal neurological deficits, and fatal hemorrhagic stroke. Human genetic studies have identified 3 genes associated with CCM, and biochemical and molecular studies in mice have elucidated signaling pathways with important therapeutic implications. In this review, the authors shed light on the 3 discovered CCM genes as well as their protein products, with particular emphasis on their signal transduction pathways and their interaction with one another. Close focus is directed at mice model studies involving the Ccm2 gene product signaling pathway, revealing an important role for the use of simvastatin or other RhoA inhibitors as a therapeutic modality in the treatment of CCM. The remaining challenges to creating a more faithful CCM animal model as well as future clinical and research implications are reviewed.
Intramedullary spinal cord cavernous malformations
Authors: Gross Bradley A. , Du Rose , Popp A. John , Day Arthur L.
Published: September 2010

Although originally the subject of rare case reports, intramedullary spinal cord cavernous malformations (CMs) have recently surfaced in an increasing number of case series and natural history reports in the literature. The authors reviewed 27 publications with 352 patients to consolidate modern epidemiological, natural history, and clinical and surgical data to facilitate decision making when managing these challenging vascular malformations. The mean age at presentation was 42 years without a sex predilection. Thirty-eight percent of the cases were cervical, 57% thoracic, 4% lumbar, and 1% unspecified location. Nine percent of the patients had a family history of CNS CMs. Twenty-seven percent of the patients had an associated cranial CM. On presentation 63% of the patients had motor deficits, 65% had sensory deficits, 27% had pain, and 11% had bowel or bladder dysfunction. Presentation was acute in 30%, recurrent in 16%, and progressive in 54% of cases. An overall annual hemorrhage rate was calculated as 2.5% for 92 patients followed up for a total of 2571 patient-years. Across 24 reviewed surgical series, a 91% complete resection rate was found. Transient morbidity was seen in 36% of cases. Sixty-one percent of patients improved, 27% were unchanged, and 12% were worse at the long-term follow-up. Using this information, the authors review surgical nuances in treating these lesions and propose a management algorithm.
Positive and Negative Donor Impurity in an InAs/AlAs Quantum Wire
Authors: Arunachalam N. , Peter A. J.
Published: September 2010

Binding energies of positive and negative charged donor impurities in an InAs/AlAs cylindrical quantum wire are investigated. Numerical calculations are performed using the variational procedure within the single band effective mass approximation. We assume that the impurity is located at the axis of the wire. The interband optical transition with and without the exciton is computed as a function of wire radius. The valence-band anisotropy is included in our theoretical model by using different hole masses in different spatial directions. Neutral shallow donors comprise a positively charged donor and a single bound electron. It is observed that (i) negative trions have a higher binding energy than positive trions, (ii) the binding energy of the heavy-hole exciton is much larger than that of the light-hole exciton due to different hole mass values (iii) the exciton binding energy and the interband emission energy are both increased when the radius of the cylindrical quantum wire is decreased and (iv) the effect of exciton influences the interband emission energy. Our results are in good agreement with the recent published results. Keywords: Quantum wire; Impurity level; Binding energy; Excitons. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i3.4715                 J. Sci. Res. 2 (3), 433-441 (2010)
HEROES AND VILLAINS: PAUL TAYLOR'S LE SACREDU PRINTEMPS (THE REHEARSAL) AND OTHER AMERICAN TALES
Authors: Kane Angela
Published: September 2010

Some of the ideas outlined below were first presented at the conference, Last Looks and First Words, in San Francisco in April 1999. The three-day conference coincided with a revival of Le Sacre du Printemps (The Rehearsal) at the Ycrba Buena Center for the Arts and it brought together dance critics, musicologists, and both current and former Taylor company personnel. I am indebted to many of the participants for their generous and insightful feedback during the conference, and most particularly to Ruth Andrien, Bettie de Jong, Richard Chen See, John Tomlinson and Mr. Taylor himself Also, I am extremely grateful to Lois Greenfield, Jack Mitchell and Jack Vartoogian for granting permission to use their photographs here and to my Rochampton colleague, Damian Day, who helped me to obtain a copy of the piano version of Sacre in London and to analyse the score.
Synthesis of 5-(6-hydroxy-7H-purine-8-ylthio)- 2-(N-hydroxyformamido)pentanoic acid
Authors: Zhang Yanmei , Elliot Greg , Saldanha Adrian , Tsigelny Igor , Carson Dennis , Wrasidlo Wolf
Published: September 2010

We have developed a synthetic route for the preparation of a hybrid bisubstrate small molecule based on a nucleoside. A prototype compound was designed and docked into the catalytic domain of the AdSS enzyme bridging the region between the magnesium center of the protein to the nucleoside region. The synthesis involves coupling a brominated peptide fragment capable of complexing magnesium to a thiolated nucleoside to obtain the hybrid model compound.
Effect of Terminalia catappa Fruit Meal Fermented by Aspergillus niger as Replacement of Maize on Growth Performance, Nutrient Digestibility, and Serum Biochemical Profile of Broiler Chickens
Authors: Apata David Friday
Published: September 2010

A feeding experiment was conducted to investigate the effect of fermented Terminalia catappa fruit meal (FTCM) with Aspergillus niger as replacement for maize on broiler growth performance, nutrient digestibility, and serum biochemical constituents. Dietary maize was replaced by FTCM at 0, 20, 40, 60, or 80%. One hundred and eighty one-day-old Shaver broiler chicks were randomly allocated to the five dietary treatments, three replicate groups of twelve chicks each for a 42-day period. There was no significant difference () in the feed intake, weight gain, and feed; gain ratio between the broilers fed on 40% FTCM diet and the control group. The apparent digestibilities of nitrogen, crude fibre, and fat decreased significantly in broilers fed higher levels (40%) of FTCM replacement diets compared with the control or lower FTCM diets. Serum concentrations of total protein, albumin, and globulin were decreased () on 80% FTCM fed broilers. Serum cholesterol, creatinine, and glucose were not significantly () altered among treatments. The activities of aspartate and alanine aminotransferases and alkaline phosphatase were significantly () increased with higher FTCM replacement. The results indicate that FTCM could replace up to 40% of dietary maize in the diets of broiler chickens without adverse effect on growth performance or serum constituents.
Health Coaching to Improve Healthy Lifestyle Behaviors: An Integrative Review
Authors: Olsen Jeanette M. , Nesbitt Bonnie J.
Published: September 2010

Objective:

Chronic diseases account for 70% of U.S. deaths. Health coaching may help patients adopt healthy lifestyle behaviors that prevent and control diseases. This integrative review analyzed health coaching studies for evidence of effectiveness and to identify key program features.

Data Source:

Multiple electronic databases were utilized, yielding a final sample of 15 documents.

Study Inclusion and Exclusion Criteria:

The search was limited to peer-reviewed research articles published between 1999 and 2008. Studies were further analyzed if they (1) specifically cited coaching as a program intervention, and (2) applied the intervention to research.

Data Extraction:

Articles describing various quantitative and qualitative methodologies were critically analyzed using a systematic method.

Data Synthesis:

Data were synthesized using a matrix format according to purpose, method, intervention, findings, critique, and quality rating.

Results:

All 15 studies utilized nonprobability sampling, 7 (47%) with randomized intervention and control groups. Significant improvements in one or more of the behaviors of nutrition, physical activity, weight management, or medication adherence were identified in six (40%) of the studies. Common features of effective programs were goal setting (73%), motivational interviewing (27%), and collaboration with health care providers (20%).

Conclusions:

Health coaching studies with well-specified methodologies and more rigorous designs are needed to strengthen findings; however, this behavioral change intervention suggests promise.
Uncertainties in using remote sensing for water use determination: a case study in a heterogeneous study area in South Africa
Authors: Gibson L. A. , Münch Z. , Engelbrecht J. , Conrad J. E.
Published: September 2010

Abstract. South Africa is a water scarce country where it is important for water managers to have accurate information on water resource occurrence and use. A remote sensing project highlighted many uncertainties in using complex remote sensing models to determine water use in a heterogeneous study area. The severity of the uncertainties was confirmed as the results across the catchment showed a higher total evapotranspiration than precipitation. This paper illustrates some of the uncertainties and limitations using the evapotranspiration component of the water balance as calculated by the Surface Energy Balance System (SEBS) model, as an example. The introduction of uncertainties in the derivation of evapotranspiration were identified as: (1) sensitivity to land surface and air temperature gradient; (2) the choice of fractional vegetation cover formula; (3) height of wind speed measurement in relation to displacement height indicating a maximum canopy height at which the SEBS model should be used; and (4) study area heterogeneity. Uncertainties and errors are compounded when considering that the SEBS model is a complex model, requiring several image processing sequences that are combined to produce the final result. It was shown how the production and propagation of errors in the SEBS model can contribute to uncertainties in flux estimation and ultimately to uncertainties in the estimation of actual evapotranspiration.
Egeson's (George's) transtridecadal weather cycling and sunspots
Authors: Halberg F. , Cornélissen G. , Bernhardt K.-H. , Sampson M. , Schwartzkopff O. , Sonntag D.
Published: September 2010

Abstract. In the late 19th century, Charles Egeson, a map compiler at the Sydney Observatory, carried out some of the earliest research on climatic cycles, linking them to about 33-year cycles in solar activity, and predicted that a devastating drought would strike Australia at the turn of the 20th century. Eduard Brückner and William J. S. Lockyer, who, like Egeson, found similar cycles, with notable exceptions, are also, like the map compiler, mostly forgotten. But the transtridecadal cycles are important in human physiology, economics and other affairs and are particularly pertinent to ongoing discusions of climate change. Egeson's publication of daily weather reports preceded those officially recorded. Their publication led to clashes with his superiors and his personal life was marked by run-ins with the law and, possibly, an implied, but not proven, confinement in an insane asylum and premature death. We here track what little is known of Egeson's life and of his bucking of the conventional scientific wisdom of his time with tragic results.
Spatial structures and directionalities in Monsoonal precipitation over South Asia
Authors: Malik N. , Marwan N. , Kurths J.
Published: September 2010

Abstract. Precipitation during the monsoon season over the Indian subcontinent occurs in form of enormously complex spatiotemporal patterns due to the underlying dynamics of atmospheric circulation and varying topography. Employing methods from nonlinear time series analysis, we study spatial structures of the rainfall field during the summer monsoon and identify principle regions where the dynamics of monsoonal rainfall is more coherent or homogenous. Moreover, we estimate the time delay patterns of rain events. Here we present an analysis of two separate high resolution gridded data sets of daily rainfall covering the Indian subcontinent. Using the method of event synchronization (ES), we estimate regions where heavy rain events during monsoon happen in some lag synchronised form. Further using the delay behaviour of rainfall events, we estimate the directionalities related to the progress of such type of rainfall events. The Active (break) phase of a monsoon is characterised by an increase(decrease) of rainfall over certain regions of the Indian subcontinent. We show that our method is able to identify regions of such coherent rainfall activity.
Laboratory Growth and Maintenance of Haemophilus influenzae
Authors: Johnston Jason W.
Published: September 2010

Haemophilus influenzae is a non‐spore forming, non‐motile, pleomorphic Gram‐negative coccobacilli. H. influenzae is a strict human pathogen that can cause a variety of systemic and localized infections, depending on the strain. The three Basic Protocols in this unit will describe how to culture and prepare H. influenzae for the use in biological studies of the organism. Curr. Protoc. Microbiol. 18:6D.1.1‐6D.1.5. © 2010 by John Wiley & Sons, Inc.
Laboratory Maintenance of Streptomyces Species
Authors: Shepherd Micah D. , Kharel Madan K. , Bosserman Mary A. , Rohr Jürgen
Published: September 2010

This unit includes general protocols for the laboratory maintenance of Streptomyces species, including growth in liquid media, growth on solid agar, and short‐ and long‐term storage. Considerations for the handling of Streptomyces species and the morphology of the bacteria are also reviewed. Curr. Protoc. Microbiol. 18:10E.1.1‐10E.1.8. © 2010 by John Wiley & Sons, Inc.
Hamiltonian techniques for the problem of set‐membership state estimation
Authors: Kurzhanski A. B.
Published: September 2010

The problem of filtering under unknown input disturbances is addressed with set‐membership bounds on the uncertain items. The possibility of solving this problem is considered using techniques of dynamic programming in continuous time via the related Hamilton–Jacobi–Bellman equations. The exact solutions to this problem, given in set‐theoretic terms as ‘information sets’, are expressed as level sets to the solutions of some specific types of the HJB equation which are given in two alternative versions. The suggested equations apply not only to the linear but also to the nonlinear case. However, in the nonlinear case the equations are especially difficult to calculate. This paper presents an alternative approach, based on a comparison principle that avoids exact solutions in favor of their upper and lower bounds, which in many cases may suffice for solving the required problems. For systems with linear structure the comparison principle yields a parameterized array of ellipsoidal estimates, which ensure tight approximations of the convex information sets. It also indicates a deductive scheme for deriving these estimates in contrast with the earlier inductive schemes. Copyright © 2010 John Wiley & Sons, Ltd.
Highly efficient orthopalladated complexes of second and third benzyl amine for catalyzing the Suzuki cross‐coupling reaction
Authors: Karami Kazem , Salah Mina Mohamadi
Published: September 2010

In this work, ortho‐palladated complexes [Pd(µ‐Cl)(C6H4CH2 NRR′‐κ2‐C,N)]2 and [Pd(C6H4CH2NH2‐2‐C,N)Cl(Y)] were tested in the Suzuki–Miyaura cross‐coupling reaction. Cyclopalladated Pd(II) complexes as thermally stable catalysts can activate aryl bromides and chlorides. These complexes were active and efficient catalysts for the Suzuki–Miyaura reaction of aryl bromides and even less reactive aryl chlorides. The cross‐coupled products of a variety of aryl bromides and aryl chloride with phenylboronic acid in methanol as solvent at 60 °C were produced in excellent yields. Copyright © 2010 John Wiley & Sons, Ltd.
The Janus face of pluripotent stem cells – Connection between pluripotency and tumourigenicity
Authors: Wobus Anna M.
Published: September 2010

Pluripotent stem cells have gained special attraction because of their almost unlimited proliferation and differentiation capacity in vitro. These properties substantiate the potential of pluripotent stem cells in basic research and regenerative medicine. Here three types of in vitro‐cultured pluripotent stem cells (embryonic carcinoma, embryonic stem and induced pluripotent stem cells) are compared in their historical context with respect to their different origin and properties. It became evident that tumourigenicity is an inherent property of pluripotent cells based on p53 down‐regulation, expression of tumour‐related genes and high telomerase activity that allow unlimited proliferation. In addition, culture‐adapted genetic and epigenetic changes may induce tumourigenicity of pluripotent cells. The use of stem cells in regenerative medicine, however, requires non‐malignant cell types and strategies that circumvent stages of malignancy. Reprogramming strategies of adult somatic cells that avoid the tumourigenic state of pluripotency may offer alternatives for future biomedical application.
Quantitative influence of endogenous salicylic acid level on taxuyunnanine C biosynthesis in suspension cultures of Taxus chinensis
Authors: Zhou Xin , Zhong Jian‐Jiang
Published: September 2010

Intracellular signals are critical to secondary metabolite biosynthesis by plant cells, but their quantitative effects are not yet well understood in plant cell cultures. Using Taxus chinensis suspension culture, which has the potential to provide a sustainable supply of highly useful bioactive taxoids, this work investigated the impact of endogenous salicylic acid (SA) level on taxuyunnanine C (Tc) biosynthesis. It was observed that the Tc accumulation was strongly dependent on the endogenous SA level. Under elicitation with an endogenous SA of 97.1 ± 11.8 µg/g dry weight, a maximal Tc content of 10.3 ± 0.52 mg/g dry weight was obtained. The transcription levels of Tc biosynthetic genes of geranylgeranyl diphosphate synthase and taxadiene synthase were also analyzed, and they were increased with increase of internal SA level. The results demonstrated that manipulation of endogenous SA level could be an efficient strategy for improving secondary metabolite biosynthesis in plant cell culture. Biotechnol. Bioeng. 2011; 108:216–221. © 2010 Wiley Periodicals, Inc.
Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine
Authors: Qian Zhi‐Gang , Xia Xiao‐Xia , Lee Sang Yup
Published: September 2010

A five carbon linear chain diamine, cadaverine (1,5‐diaminopentane), is an important platform chemical having many applications in chemical industry. Bio‐based production of cadaverine from renewable feedstock is a promising and sustainable alternative to the petroleum‐based chemical synthesis. Here, we report development of a metabolically engineered strain of Escherichia coli that overproduces cadaverine in glucose mineral salts medium. First, cadaverine degradation and utilization pathways were inactivated. Next, L‐lysine decarboxylase, which converts L‐lysine directly to cadaverine, was amplified by plasmid‐based overexpression of the cadA gene under the strong tac promoter. Furthermore, the L‐lysine biosynthetic pool was increased by the overexpression of the dapA gene encoding dihydrodipicolinate synthase through the replacement of the native promoter with the strong trc promoter in the genome. The final engineered strain was able to produce 9.61 g L−1 of cadaverine with a productivity of 0.32 g L−1 h−1 by fed‐batch cultivation. The strategy reported here should be useful for the bio‐based production of cadaverine from renewable resources. Biotechnol. Bioeng. 2011; 108:93–103. © 2010 Wiley Periodicals, Inc.
Effects of solution environment on mammalian cell fermentation broth properties: Enhanced impurity removal and clarification performance
Authors: Westoby Matthew , Chrostowski James , de Vilmorin Philippe , Smelko John Paul , Romero Jonathan K.
Published: September 2010

The processing of recombinant proteins from high cell density, high product titer cell cultures containing mammalian cells is commonly performed using tangential flow microfiltration (MF). However, the increased cellular debris present in these complex feed streams can prematurely foul the membrane, adversely impacting MF capacity and throughput. In addition, high cell density cell culture streams introduce elevated levels of process‐related impurities, which increase the burden on subsequent purification operations to remove these complex media components and impurities. To address this challenge, an evaluation of mammalian cell culture broth buffer properties was examined to determine if enhanced impurity removal and clarification performance could be achieved. A framework is presented here for establishing optimized mammalian cell culture buffer conditions, involving trade‐offs between product recovery and purification and improved clarification at manufacturing‐scale production. A reduction in cell culture broth pH to 4.7–5.0 induced flocculation and impurity precipitation which increased the average feed particle‐size. These conditions led to enhanced impurity removal and improved MF throughput and filter capacity for several mammalian systems. Feed conditions were further optimized by controlling ionic composition along with pH to improve product recovery from high cell density/high product titer cell cultures. Biotechnol. Bioeng. 2011; 108:50–58. © 2010 Wiley Periodicals, Inc.
Determination of anthocyanins in the urine of patients with colorectal liver metastases after administration of bilberry extract
Authors: Cai Hong , Thomasset Sarah C. , Berry David P‐. , Garcea Giuseppe , Brown Karen , Steward William P. , Gescher Andreas J.
Published: September 2010

Anthocyanins possess cancer chemopreventive properties in preclinical models. Their clinical pharmacology is only poorly understood. In this pilot study, anthocyanins and their metabolites were analysed in the urine of two patients with colorectal liver metastases. They received a single dose of 1.88 g standardized bilberry extract (mirtoselect) via either nasogastric or nasojejunal tube intra‐operatively during liver resection. HPLC‐MS/MS and HPLC‐UV analysis showed there were more anthocyanins and metabolites in the urine of the patient who received mirtoselect via the stomach than via the jejunum. This result is consistent with information obtained in rodents which suggests the stomach is the predominant site for anthocyanin absorption. Copyright © 2010 John Wiley & Sons, Ltd.
An LC‐MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study
Authors: Zhou Dandan , Tong Ling , Wan Meixu , Wang Gangli , Ye Zhengliang , Wang Zhengtao , Lin Ruichao
Published: September 2010

A sensitive and reliable LC‐ESI‐MS method for simultaneous determination of nine ginsenosides (Rh1, Rg2, Rg1, Rf, Re, Rd, Rc, Rb2 and Rb1) in rat plasma was developed and validated using saikosaponin A as an internal standard. The samples were extracted by solid‐phase extraction. Chromatographic separation was carried out on a Hypersil Gold C18 column (100 × 2.1 mm, 5 µm) by stepwise gradient elution with water (0.1% formic acid, v/v) and acetonitrile as the mobile phase. Detection was determined by selective ion monitoring mode using electrospray ionization in the negative ion mode. Good linearity over the investigated concentration ranges was observed with the values of r higher than 0.9900. The intra‐ and inter‐day precisions were all no more than 15% and the average recoveries varied from 71.8 to 91.7%. This quantitative measurement was successfully applied to a pharmacokinetic study of Yi‐Qi‐Fu‐Mai injection. Copyright © 2010 John Wiley & Sons, Ltd.
A rapid and simple assay for lamotrigine in serum/plasma by HPLC, and comparison with an immunoassay
Authors: Morgan Phillip E. , Fisher Danielle S. , Evers Richard , Flanagan Robert J.
Published: September 2010

Monitoring serum/plasma concentrations of lamotrigine may be useful under certain circumstances. An HPLC column packed with strong cation‐exchange (SCX)‐modified microparticulate silica together with a 100% methanol eluent containing an ionic modifier permits direct injection of sample extracts. An HPLC‐UV method developed using this principle for the measurement of serum/plasma lamotrigine is simple, sensitive and selective. The analysis time is less than 5 min. Intra‐ and inter‐assay precision and accuracy meet acceptance criteria, and sample stability, and potential interferences from other compounds have been evaluated. There was good agreement with consensus mean results from external quality assessment samples (n = 32). Analysis of patient samples (n = 115) using the HPLC method and the Seradyn QMS® Lamotrigine immunoassay showed that the immunoassay over‐estimated lamotrigine by 21% on average. Copyright © 2010 John Wiley & Sons, Ltd.
ChemInform Abstract: Low‐Pressure Chemical Vapor Deposition of Silicon Dioxide Using Diethylsilane.
Authors: LEVY R. A. , GROW J. M. , CHAKRAVARTHY G. S.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Preparation of Sodium Tungsten Bronze (cubic) by the Reduction of Tungstates with Hydrogen.
Authors: KONDO W. , MANABE T. , KUMAGAI T. , MIZUTA S.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: An Easy and Fast Way to Determine the Enantiomeric Purity of Substituted Cyclanones.
Authors: ALEXAKIS A. , FRUTOS J. C. , MANGENEY P.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reactions of Methyl(2,5,5‐trimethyl‐1‐cyclopenten‐1‐yl)carbene and the Photochemistry of 2‐(1‐Propynyl)‐2,5,5‐trimethylcyclopentanone.
Authors: VENUGOPAL D. , MARGARETHA P. , AGOSTA W. C.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Kinetics and Mechanism of Iridium(III) Catalyzed Oxidation of Aliphatic Amines by N‐Bromosuccinimide.
Authors: SHUKLA A. , UPADHYAY S. K.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: N‐Oxopiperidinium Salt‐Assisted Electrochemical and Chemical Oxidation of Alcohols in a Two‐Phase System: Synthesis of 4‐Chlorobutanal, Formylcyclopropanes, and m‐Phenoxybenzaldehyde.
Authors: OGIBIN YU. N. , KHUSID A. KH. , NIKISHIN G. I.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Amination of Nitrobenzene via Nucleophilic Aromatic Substitution for Hydrogen: Direct Formation of Aromatic Amide Bonds.
Authors: STERN M. K. , CHENG B. K.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reaction of 1‐Chloro‐2‐methyl‐1‐propenyllithium with a Selection of Organolithiums. The Development and Synthetic Utility of Novel Base/ Nucleophile Combinations.
Authors: NELSON D. J. , NAGARAJAN A.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Synthesis and Transition Temperatures of Some Difluorosubstituted Cyclohexanes.
Authors: HIRD M. , TOYNE K. J. , SLANEY A. J. , GOODBY J. W. , GRAY G. W.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: α‐Methylenelactam Synthesis via Radical Cyclization of Propiolamide.
Authors: LEE E. , KANG T. S.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 3‐Triazenopyrroles: Synthesis and Antineoplastic Activity.
Authors: DATTOLO G. , CIRRINCIONE G. , ALMERICO A. M. , AIELLO E. , GRIMAUDO S. , DIANA P. , RAUSA L. , DUSONCHET L. , CROSTA L. , TOLOMEO M. , CANDILORO V. , MELI M.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Gem‐Dinitro Compounds in Organic Synthesis. Part 3. Syntheses of 4‐ Nitro‐1,2,3‐triazoles from gem‐Dinitro Compounds.
Authors: BARYSHNIKOV A. T. , ERASHKO V. I. , ZUBANOVA N. I. , UGRAK B. I. , SHEVELEV S. A. , FAINZIL'BERG A. A. , LAIKHTER A. L. , MEL'NIKOVA L. G. , SEMENOV V. V.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Local Anesthetic Activity of Alkylaminoacyl Derivatives of 2‐Amino‐1,3,4‐thiadiazole.
Authors: MAZZONE G. , PIGNATELLO R. , MAZZONE S. , PANICO A. , PENNISI G. , CASTANA R. , MAZZONE P. MAZZONE P.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Antimicrobial Activity of DNA‐Gyrase Inhibiting Derivatives of 4‐Oxo‐1,4‐dihydro‐3‐pyridinecarboxylic Acid.
Authors: BASSINI C. , BISMARA C. , CARLESSO R. , FERIANI A. , GAVIRAGHI G. , MARCHIORO C. , PERBONI A. , SHAW R. E. , TAMBURINI B. , TARZIA G. , XERRI L.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Selective Reactions Using N‐(tert‐Butoxycarbonyl)‐2‐(tert‐ butyldimethylsiloxy)pyrrole: Concise Asymmetric Syntheses of (+)‐1‐ Deoxy‐8‐epi‐castanospermine (VII) and Its Enantiomer.
Authors: CASIRAGHI G. , ULGHERI F. , SPANU P. , RASSU G. , PINNA L. , GASPARRI FAVA G. , BELICCHI FERRARI M. , PELOSI G.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Some New 1,2,4‐Triazines Containing Aminopyrimidine Moiety.
Authors: SEADA M. , ABDEL‐RAHMAN R. M. , HANAFY F.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Opioid Receptor Affinity of Bivalent Ligands Derived from 3,8‐Diazabicyclo(3.2.1)octanes.
Authors: BARLOCCO D. , FADDA P. , FRATTA W.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Spectral Studies of 3‐Aryloxy‐2‐ benzylnaphthoxazaphosphorine‐3‐oxides and 2‐(4‐Methylphenyl) naphthoxazaphosphorine‐3‐sulfides.
Authors: NAIDU M. S. R. , PRASAD M. V. S. R.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chiral Diphospholes. Part 3. Improved Synthesis and Coordination Chemistry of (R,R)‐DIPPOP.
Authors: BRUNET J.‐J. , GOMEZ M. , HAJOUJI H. , NEIBECKER D.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of 1‐Phosphaspiro(2.n)alkanes by Addition of Terminal Phosphinidene Complexes to Exocyclic Olefins.
Authors: HUNG J.‐T. , YANG S.‐W. , GRAY G. M. , LAMMERTSMA K.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Simple Cycloplatination of Triphenylphosphine.
Authors: SCHEFFKNECHT C. , RHOMBERG A. , MUELLER E. P. , PERINGER P.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Molecular Rearrangements of D:A‐Friedoolean‐6α,7α‐epoxide.
Authors: MUKHERJEE J. , DUTTAGUPTA A. , SAYEED A.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Analogues of Squalene as Potential Antifungal Agents.
Authors: DURIATI A. , JUNG M. , MANN J. , SMITH G. P.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Carboranyl Oligonucleotides. Part 1. Synthesis of Thymidine(3′,5′) thymidine (o‐Carboran‐1‐ylmethyl)phosphonate.
Authors: LESNIKOWSKI Z. J. , SCHINAZI R. F.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nomenclature for Liquid‐Liquid Distribution (Solvent Extraction).
Authors: RICE N. M. , IRVING H. M. N. H. , LEONHARD M. A.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nomenclature and Therminology for Analytical Pyrolysis.
Authors: UDEN P. C.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Studies on Proton Conducting Ceramics Based on Perovskite‐Type Oxides.
Authors: IWAHARA H. , HIBINO T. , YAJIMA T.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Crystal Structure of the Novel Cyclic Germanate K3.8Nb5(Ge3O9)O11.4.
Authors: BELOKONEVA E. L. , MILL' B. V. , BUTASHIN A. V.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.