bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of α-Naphthylacetic Acid on Cadmium Stress and Related Factors of Tomato by Regulation of Gene Expression
Authors: Guan Xiaoxi , Sui Changling , Luo Kecui , Chen Zhifeng , Feng Chaoyang , Dong Xiufen , Zeng Boping , Dong Xian , Liu Xiaofang
Published: September 2022

Cadmium (Cd) is absorbed and accumulated by crops, and it adversely affects plant growth and development. To explore the effect of exogenous auxin on Cd stress, we applied different concentrations of α-naphthaleneacetic acid (NAA) and the auxin transport inhibitor 2,3,5-triiodobenzoic acid (TIBA) to tomato plants exposed to Cd stress in a hydroponic system. NAA and TIBA at different concentrations were used under Cd stress. Plant growth, root morphology, and auxin distribution were observed. Lipid peroxidation and antioxidant enzyme activities in leaves, cadmiumcontent, and migration coefficient of plants were determined. Transcriptome sequencing and qRT-PCR were used to analyze the differentially expressed genes. Results showed that auxin was concentrated in the leaf veins, stem base, and roots in P5::GUS “Chico III” transgenic tomato, indicating NAA polar transport to the roots and promotion of root growth under Cd stress. Cd was absorbed by the roots and transported to the shoots. It then inhibited plant growth and promoted antioxidant enzyme activities, O2− production, H2O2 accumulation, and membrane lipid peroxidation. Treatment with 0.5 μM NAA improved antioxidant enzyme activities, reduced reactive oxygen, maintained membrane permeability, and decreased malondialdehyde and proline contents. Transcriptome analysis revealed that NAA activated a large number of genes in the roots: 1998 genes were differentially expressed in response to Cd or NAA treatment, and 1736 genes were specifically expressed in response to NAA treatment under Cd stress. Among the differentially expressed genes, tomato metallocarboxypeptidase inhibitor TCMP-2 (2A11) and Solanum lycopersicum heavy metal-associated isoprenylated plant protein (HIPP) 7-like (LOC101264884), which are closely related to plant response to heavy metal stress, may be the key sites of NAA. In conclusion, the NAA-mediated response to Cd stress was closely associated with “defense response” genes in shoots and “oxidoreductase activity, oxidizing metal ions” and “response to auxin” genes in roots.
Identification and Quantification of Actual Evapotranspiration Using Integrated Satellite Data for Sustainable Water Management in Dry Areas
Authors: Gamal Rania , El-Shirbeny Mohamed , Abou-Hadid Ayman , Swelam Atef , El-Gindy Abdel-Ghany , Arafa Yasser , Nangia Vinay
Published: September 2022

Evapotranspiration (ET) is a significant consumer of irrigation water and precipitation on cropland. Global and regional interest in the sustainable management of limited freshwater supplies to meet the rapidly increasing population and food demands has resulted in advanced scientific research on ET measurement, rapid water accounting, and irrigation schedules in the NENA region. The primary goal of this paper is to compare actual daily evapotranspiration (ET) collected by a remote sensing model and validated by Energy Balance (EB) flux tower field measurements. The flux tower was installed in a wheat field in Sids Agricultural Research Station in Beni Suef Governorate. Through the integration of Moderate Resolution Imaging Spectroradiometer (MODIS) Terra and Sentinel-2 data, a new remote sensing-based ET model is built on two parties: Thermal condition factor (TCF) and vegetation condition fraction (VCF). The remote sensing-based ET estimation model was evaluated using ET field measurements from the Energy Balance flux tower. The land use and land cover maps were created to assist the interpretation of remotely sensed ET data. Field data for five categories were collected to test the accuracy of the land use and cover maps: Water bodies (93 points), urban areas (252 points), trees (104 points), other field crops (227 points), and wheat (249 points), for a total of 925 ground points. The Google Earth Engine (GEE) imported sentinel-2 datasets and filtered the necessary dates and regions. From 1 October 2020 to 30 May 2021, sentinel-2 data were processed and transformed into the Normalized Difference Vegetation Index (NDVI), Normalized Difference Water Index (NDWI), and Normalized Difference Built-up Index (NDBI), which were then combined. The composite layer data were classified using the Random Forest (RF) method on the GEE platform, and the results showed an overall accuracy of 91 percent. The validation factors revealed good indices when RS-based ET results were compared to ground-measured ET. The Root Mean Square Error (RMSE) was 0.84 mm/day. The ‘r’ and ‘d’ values indicated satisfactory results, where ‘r’ yielded a value of 0.785, which indicates that the correlation between predicted and reference results is robust. The analysis of d values revealed a high degree of correlation between predicted (RS-based ET) and reference results (measured ET). The d value was found to be 0.872. Between 21 November 2020 and 30 April 2021, RS-based accumulated ET was 418 mm/season, while ground-measured ET was 376 mm/season. The new RS-based ET model produced acceptable daily and seasonal results.
Variation in Physical-Chemical Parameters and Phenolic Compounds in Fruits of Four Calafate Clones
Authors: Pinto-Morales Fernando , Retamal-Salgado Jorge , López María Dolores , Zapata Nelson , Vergara-Retamales Rosa , Palma Daniela
Published: September 2022

Calafate (Berberis microphylla G. Forst) is an evergreen shrub with blue berries that grows naturally in Patagonia, in South America. It has beneficial nutraceutical characteristics for human health. The objective of the research was to evaluate the effect of different harvest dates of calafate clones in the south-central zone of Chile on the polyphenolic content, antioxidant capacity, quality parameters and fruit yield. To meet this objective, during three consecutive years, four wild calafate clones located in the town of San Ignacio, Chile, were selected. Where a harvest period was established from 110 to 140 days after full bloom (DAFBs), each of the harvests carried out were used for the following measurements: antioxidant capacity, determination of anthocyanin content, concentration of polyphenols, phenolic compounds, soluble solids, total titratable acidity, pH, fruit yield and quality. Among the main results, it can be highlighted that clone 2 was the one that obtained the highest concentration of soluble solids, with 38.0 °Brix at 140 DAFBs. Together, it was the one that obtained the highest content of total polyphenols and concentration of anthocyanins, with 1121 g GAE kg−1 fw and 714 g cy-3-glu 100 g−1 fw, respectively.
A New Fertilization Approach for Bread Wheat in the Mediterranean Environment: Effects on Yield and Grain Protein Content
Authors: Mathlouthi Fakir , Ruggeri Roberto , Rossini Angelo , Rossini Francesco
Published: September 2022

Plant biostimulants represent an innovative and sustainable solution to address the challenges of the future agriculture, especially when they are used to improve yield and quality of staple crops. The objective of this research was to study, over three consecutive seasons, the effect of a novel fertilization plan (Thesis 2, T2) on the productivity and protein content of bread wheat (Triticum aestivum L.), as compared to the traditional fertilization pattern (Thesis 1, T1), commonly used in Tunisia. T2 was based on the use of a pre-sowing soil bioenhancer (SBE, commercially known as ‘Terios’) and a topdressing with foliar bio-stimulant (FBS, commercially known as ‘Celerios’), obtained by nanotechnology transformation of Y-CaCO3 minerals (called ‘vaterite’); while T1 was based on the use of diammonium phosphate (DAP) at pre-sowing and ammonium nitrate (AN) during the growing season. FBS was applied two times each season and at one rate (3 kg ha−1). In each farm and experimental year, the following traits were recorded: plant height (cm), whole aerial biomass (t DM ha−1), grain yield (t ha−1, 13% moisture content), harvest index, grain weight (mg), spike density (number of spikes per m2), grain protein content (%). T2 protocol slightly, but significantly, increased yield, yield components and grain protein content, while it decreased plant height at harvest. These results suggest that the use of FBS could be of great interest for the cultivation of bread wheat under Mediterranean climatic conditions, as it can make plant nutrients rapidly available even when the uptake from the soil is hindered by water scarcity.
Influence of Micro-Furrow Depth and Bottom Width on Surface Water Flow and Irrigation Performance in the North China Plain
Authors: Zai Songmei , Feng Xuefang , Wang Donglin , Zhang Yan , Wu Feng
Published: September 2022

Improving traditional surface irrigation technology and vigorously promoting water-saving surface irrigation are important ways to improve the efficiency of water resource utilization. In our study, we propose a new technology of surface irrigation, micro-furrow irrigation, which combines the advantages of furrow irrigation and border irrigation. The objective of this experiment was to evaluate the effects of micro-furrow depth and bottom width on surface water flow and irrigation performance. Field experiments were conducted from 2019 to 2020 in Zhengzhou City, northern China. This work designed three bottom widths, BW1 (18 cm), BW2 (12 cm), and BW3 (6 cm), respectively, and three depths, D1 (15 cm), D2 (10 cm), and D3 (5 cm), respectively. Moreover, border irrigation was set as control treatment (CK). Additionally, field experiments were validated and simulated using the WinSRFR 5.1 model (Arid-Land Agricultural Research Center, USA). The results showed a significant negative correlation between depth and advance time and between depth and recession time. However, no significant correlation was found between bottom width and advance time, nor between bottom width and recession time. The advance times of micro-furrow irrigation were 1.23–4.77 min less than those of border irrigation. Concerning irrigation performance, compared to that of border irrigation, the application efficiency and distribution uniformity increased by 8–30% and −5–18%, respectively. However, the requirement efficiency decreased by 0–40%. Compared to that of border irrigation, the irrigation quota increased 21.61% under BW3D3 but decreased by 10.46–57.94% under other treatments. Therefore, micro-furrow irrigation can meet irrigation requirements despite low irrigation quota. Comprehensively considering the advance time, recession time, irrigation performance, and irrigation quota, we recommend a micro-furrow shape with a depth of 10 cm or 15 cm and bottom width of 6 cm.
In Vitro Effects of Short-Term and Long-Term Heat Exposures on the Immune Response and Prostaglandin Biosynthesis in Bovine Endometrial Cells
Authors: Chotimanukul Sroisuda , Suwimonteerabutr Junpen , Techakumphu Mongkol , Swangchan-Uthai Theerawat
Published: September 2022

Worldwide heat stress (HS) conditions have a negative impact on dairy cow fertility. However, understanding of the effect of heat stress on endometrial functions is still unclear. The present study aimed to investigate the effects of differential heat exposure conditions on the immune response and prostaglandin biosynthesis of bovine endometrium challenged with bacterial lipopolysaccharide (LPS). Cultures of endometrial cells were grown to confluence at 37 °C (control) and 40.4 °C for 24 h after confluence (short-term heat exposure) and 40.4 °C for 8 days from the beginning of the culture (long-term heat exposure), prior to a challenge by 100 ng/mL LPS for 12 h. LPS altered ALOX12, IL8, IL1B, S100A8, PTGES and AKR1B1 expressions, as well as secretory IL8 and PGF2α. Short-term heat exposure decreased S100A8, IL8 and PGF2α compared with the control temperature, while long-term heat exposure decreased S100A8 and PGF2α. In contrast, HSPA5 expression was not altered by heat exposure or LPS. Indeed, the short-term heat treatment was insufficient for accomplishing the responses of the endometrium to LPS treatment for IL8, S100A8 and PTGES expressions when compared with other temperature conditions. Our findings showed that heat exposure could compromise endometrium immune response and prostaglandin biosynthesis in different ways based on elevated temperature duration, which could reduce subsequent fertility.
Characterization of Gut Microbiome in the Mud Snail Cipangopaludina cathayensis in Response to High-Temperature Stress
Authors: Wu Yang-Yang , Cheng Chun-Xing , Yang Liu , Ye Quan-Qing , Li Wen-Hong , Jiang Jiao-Yun
Published: September 2022

The mud snail Cipangopaludina cathayensis is a widely distributed species in China. Particularly in Guangxi province, mud snail farming contributes significantly to the economic development. However, global warming in recent decades poses a serious threat to global aquaculture production. The rising water temperature is harmful to aquatic animals. The present study explored the effects of high temperature on the intestinal microbiota of C. cathayensis. Snail intestinal samples were collected from the control and high-temperature groups on days 3 and 7 to determine the gut microbiota composition and diversity. Gut bacterial community composition was investigated using high-throughput sequencing of the V3–V4 region of bacterial 16S rRNA genes. Our results suggested that thermal stress altered the gut microbiome structure of C. cathayensis. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were dominant in C. cathayensis gut microbiota. The T2 treatment (32 ± 1 °C, day 7) significantly decreased the relative abundance of Firmicutes, Actinobacteria, and Deinococcus-Thermus. In T2, the abundance of several genera of putatively beneficial bacteria (Pseudomonas, Aeromonas, Rhodobacter, and Bacteroides) decreased, whereas the abundance of Halomonas—a pathogenic bacterial genus—increased. The functional prediction results indicated that T2 treatment inhibited some carbohydrate metabolism pathways and induced certain disease-related pathways (e.g., those related to systemic lupus erythematosus, Vibrio cholerae infection, hypertrophic cardiomyopathy, and shigellosis). Thus, high temperature profoundly affected the community structure and function of C. cathayensis gut microbiota. The results provide insights into the mechanisms associated with response of C. cathayensis intestinal microbiota to global warming.
Differences in Agonistic Behavior and Energy Metabolism between Male and Female Swimming Crab Portunus trituberculatus Based on the Analysis of Boldness
Authors: Su Xianpeng , Zhu Boshan , Ren Ziwen , Wang Fang
Published: September 2022

Individual differences in metabolism and agonistic behavior have been a key research area in evolution and ecology recently. In this study, we investigated the boldness of swimming crabs Portunus trituberculatus and explored the agonistic behavior between female and male crabs, specifically examining competitions between bold females vs. bold males (BF–BM), bold females vs. shy males (BF–SM), shy females vs. shy males (SF–SM), and shy females vs. bold males (SF_BM) and its relationship with energy metabolism. The main results revealed the following: There was no significant difference in boldness between females and males, while there were more bold individuals than shy in both females and males. Bold individuals initiated significantly more fights than shy individuals, and male initiators won significantly more fights than female initiators. The duration and intensity of fight between bold individuals was significantly higher than fights between shy individuals. For males, the concentration of glucose in the hemolymph was significantly higher in shy crabs than bold crabs, while there was no significant difference between shy and bold individuals in females. After fighting, the concentration of glycogen in claws was lower than that before fighting, and the concentrations of glucose and lactate in hemolymph were significantly higher after fighting than before. We found that the fighting willingness and ability were higher in male crabs than females and higher in bold crabs than shy. Fighting ability varied between sexes and was influenced by boldness and energy state.
Genetic Diversity and Selection Signatures in Jianchang Black Goats Revealed by Whole-Genome Sequencing Data
Authors: Sun Xueliang , Guo Jiazhong , Li Li , Zhong Tao , Wang Linjie , Zhan Siyuan , Lu Juan , Wang Decheng , Dai Dinghui , Liu George E. , Zhang Hongping
Published: September 2022

Understanding the genetic composition of indigenous goats is essential to promote the scientific conservation and sustainable utilization of these breeds. The Jianchang Black (JC) goat, a Chinese native breed, is solid black and exhibits crude feed tolerance, but is characterized by a low growth rate and small body size. Based on the whole-genome sequencing data for 30 JC, 41 Jintang Black (JT), and 40 Yunshang Black (YS) goats, and 21 Bezoar ibexes, here, we investigated the genetic composition of JC goats by conducting analyses of the population structure, runs of homozygosity (ROH), genomic inbreeding, and selection signature. Our results revealed that JT and YS showed a close genetic relationship with a non-negligible amount of gene flows but were genetically distant from JC, apart from Bezoars. An average of 2039 ROHs were present in the autosomal genome per individual. The ROH-based inbreeding estimates in JC goats generally showed moderate values ranging from 0.134 to 0.264, mainly due to rapid declines in the effective population size during recent generations. The annotated genes (e.g., IL2, IL7, and KIT) overlapping with ROH islands were significantly enriched in immune-related biological processes. Further, we found 61 genes (e.g., STIM1, MYO9A, and KHDRBS2) under positive selection in JC goats via three complementary approaches, which may underly genetic adaptations to local environmental conditions. Our findings provided references for the conservation and sustainable utilization of JC goats.
First Steps into Ruminal Microbiota Robustness
Authors: Costa-Roura Sandra , Villalba Daniel , Balcells Joaquim , De la Fuente Gabriel
Published: September 2022

Despite its central role in ruminant nutrition, little is known about ruminal microbiota robustness, which is understood as the ability of the microbiota to cope with disturbances. The aim of the present review is to offer a comprehensive description of microbial robustness, as well as its potential drivers, with special focus on ruminal microbiota. First, we provide a briefing on the current knowledge about ruminal microbiota. Second, we define the concept of disturbance (any discrete event that disrupts the structure of a community and changes either the resource availability or the physical environment). Third, we discuss community resistance (the ability to remain unchanged in the face of a disturbance), resilience (the ability to return to the initial structure following a disturbance) and functional redundancy (the ability to maintain or recover initial function despite compositional changes), all of which are considered to be key properties of robust microbial communities. Then, we provide an overview of the currently available methodologies to assess community robustness, as well as its drivers (microbial diversity and network complexity) and its potential modulation through diet. Finally, we propose future lines of research on ruminal microbiota robustness.
Diurnal and Nocturnal Behaviour of Cheetahs (Acinonyx jubatus) and Lions (Panthera leo) in Zoos
Authors: Seyrling Isabel , Dierkes Paul Wilhelm , Burger Anna Lena
Published: September 2022

Mammals are constantly exposed to exogenous and endogenous influences that affect their behaviour and daily activity. Light and temperature, as well as anthropogenic factors such as husbandry routines, visitors, and feeding schedules are potential influences on animals in zoological gardens. In order to investigate the effects of some of these factors on animal behaviour, observational studies based on the analyses of activity budgets can be used. In this study, the daily and nightly activity budgets of six lions (Panthera leo) and five cheetahs (Acinonyx jubatus) from four EAZA institutions were investigated. Focused on the influencing factor light and feeding, we analysed these activity budgets descriptively. Behaviour was recorded and analysed during the winter months over an observation period of 14 days and 14 nights using infrared-sensitive cameras. Our results show that lions and cheetahs exhibit activity peaks at crepuscular and feeding times, regardless of husbandry. Thus, lions in captivity shift nocturnal behaviour familiar from the wild to crepuscular and diurnal times. In cheetahs, in contrast, captive and wild individuals show similar 24 h behavioural rhythms. The resting behaviour of both species is more pronounced at night, with cheetahs having a shorter overall sleep duration than lions. This study describes the results of the examined animals and is not predictive. Nevertheless, the results of this study make an important contribution to gaining knowledge about possible factors influencing the behaviour of lions and cheetahs in zoos and offer implications that could be useful for improving husbandry and management.
A Practical Application of Genomic Predictions for Mastitis Resistance in Italian Holstein Heifers
Authors: Moretti Riccardo , Chessa Stefania , Sartore Stefano , Soglia Dominga , Giaccone Daniele , Cannizzo Francesca Tiziana , Sacchi Paola
Published: September 2022

Heifers are a fundamental resource on farms, and their importance is reflected in both farm management and economy. Therefore, the selection of heifers to be reared on a farm should be carefully performed to select only the best animals. Genomic selection is available nowadays to evaluate animals in a fast and economic way. However, it is mainly used on the sire line and on performance traits. Ten farms were selected based on their 5-year records of average somatic cell count and evenly classified into high (>300,000 cells/mL) and low somatic cell count (<150,000 cells/mL). Genomic indexes (regarding both wellness and productive traits) were evaluated in 157 Italian Holstein heifers reared in the selected ten farms (90 from high-cells farms and 67 from low-cells ones). Linear mixed models were fitted to analyze the effects of the abovementioned genomic indexes on related phenotypes. Results have shown that farms classified into low somatic cell count had an overall better animal genomic pool compared to high somatic cell count ones. Additionally, the results shown in this study highlighted a difference in wellness genomic indexes in animals from farms with either a high or a low average somatic cell count. Applying genomic tools directly to heifer selection could improve economic aspects related to herd turnover.
Vitamin D3 Metabolic Enzymes in Plateau Zokor (Myospalax baileyi) and Plateau Pika (Ochotona curzoniae): Expression and Response to Hypoxia
Authors: Chen Xiaoqi , An Zhifang , Wei Linna , Zhang Jiayu , Li Jimei , Wang Zhijie , Gao Conghui , Wei Dengbang
Published: September 2022

Vitamin D3 (D3) is produced endogenously from 7-dehydrocholesterol by irradiation and is an important secosteroid for the absorption of calcium and phosphate. Lithocholic acid (LCA) increases intestinal paracellular calcium absorption in a vitamin D receptor-dependent manner in vitamin D-deficient rats. The plateau zokor (Myospalax baileyi), a strictly subterranean species, and plateau pika are endemic to the Qinghai-Tibet Plateau. To verify whether the zokors were deficient in D3 and reveal the effects of hypoxia on D3 metabolism in the zokors and pikas, we measured the levels of 25(OH)D3, calcium, and LCA, and quantified the expression levels of D3 metabolism-related genes. The results showed an undetectable serum level of 25(OH)D3 and a significantly higher concentration of LCA in the serum of plateau zokor, but its calcium concentration was within the normal range compared with that of plateau pika and Sprague-Dawley rats. With increasing altitude, the serum 25(OH)D3 levels in plateau pika decreased significantly, and the mRNA and protein levels of CYP2R1 (in the liver) and CYP27B1 (in the kidney) in plateau pika decreased significantly. Our results indicate that plateau zokors were deficient in D3 and abundant in LCA, which might be a substitution of D3 in the zokor. Furthermore, hypoxia suppresses the metabolism of D3 by down-regulating the expression of CYP2R1 and CYP27B1 in plateau pika.
Positional Behavior of Introduced Monk Parakeets (Myiopsitta monachus) in an Urban Landscape
Authors: Granatosky Michael C. , Young Melody W. , Herr Victoria , Chai Chloe , Raidah Anisa , Kairo Judy Njeri , Anaekwe Adaeze , Havens Allison , Zou Bettina , Ding Billy , Chen Christopher , De Leon David , Shah Harshal , Valentin Jordan , Hildreth Lucas , Castro Taylor , Li Timothy , Yeung Andy , Dickinson Edwin , Youlatos Dionisios
Published: September 2022

Positional behaviors have been broadly quantified across the Order Primates, and in several other mammalian lineages, to contextualize adaptations to, and evolution within, an arboreal environment. Outside of Mammalia, however, such data are yet to be reported. In this study, we present the first quantitative report of positional behavior within Aves, presenting 11,246 observations of scan sampling data from a colony of Monk Parakeets (Myiopsitta monachus) from Brooklyn, New York City. Each scan recorded locomotor and postural behavior and information about weather condition, temperature, and substrate properties (e.g., type, size, orientation). A distinction was also recorded between natural and artificial substrates. Parrots exhibited a strong preference for small and terminal branches, a selection which may reflect targeted foraging of new fruit growth and leaf-buds. We further observed that the gait transition from walking to sidling appears primarily driven by substrate size, with the former preferred on the ground and on large, broad substrates and the latter used to navigate smaller branches. Finally, we observed an increase in locomotor diversity on artificial versus naturally occurring substrates. This demonstrates the importance of a flexible behavioral repertoire in facilitating a successful transition towards an urban landscape in introduced species.
Impact of an Antimicrobial Stewardship Program Intervention Associated with the Rapid Identification of Microorganisms by MALDI-TOF and Detection of Resistance Genes in ICU Patients with Gram-Negative Bacteremia
Authors: Campos Aléia Faustina , Arantes Tiago , Cambiais Amanda Magalhães Vilas Boas , Cury Ana Paula , Tiroli Camila Guimarães , Rossi Flávia , Malbouisson Luiz Marcelo Sa , Costa Silvia Figueiredo , Guimarães Thaís
Published: September 2022

Combination of strategies for rapid diagnostics tests (RDT) with real-time intervention could improve patient outcomes. We aimed to assess the impact on clinical outcomes, antimicrobial consumption, and costs in patients with gram-negative bacteremia. We designed a quasi-experimental study among 216 episodes of gram-negative bacteremia using RDT (MALDI-TOF and detection of resistance genes) directly from blood culture bottles combined with real-time communication of results. Our study did not demonstrate impact on 30-day mortality (25% vs. 35%; p = 0.115). Hospital and ICU length of stay were significantly lower in the intervention period ((44 days vs. 39 days; p = 0.005) and (17 days vs. 13 days; p = 0.033)), respectively. The antimicrobial consumption was 1381 DOT/1000 days in the pre-intervention period compared to 1262 DOT/1000 days in the intervention period (p = 0.032). Antimicrobials against gram-positive and carbapenems had a significantly reduced consumption in the intervention period. Our intervention showed no impact on 30 days-mortality, but demonstrated an impact on hospital and ICU length of stay, as well as antimicrobials consumption and costs. Knowledge of resistance genes adds value and information for safe decision making that can result in direct and indirect benefits related to the economic burden of antibiotic overuse and bacterial resistance.
Predictors of Vancomycin-Resistant Enterococcus spp. Intestinal Carriage among High-Risk Patients in University Hospitals in Serbia
Authors: Janjusevic Ana , Cirkovic Ivana , Minic Rajna , Stevanovic Goran , Soldatovic Ivan , Mihaljevic Biljana , Vidovic Ana , Markovic Denic Ljiljana
Published: September 2022

The predictors of intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high-risk patients in the counties of the Southeast Europe Region are insufficiently investigated, yet they could be of key importance in infection control. The aim of the study was to identify risk factors associated with fecal VRE colonization among high-risk inpatients in university hospitals in Serbia. The study comprised 268 inpatients from three university hospitals. Data on patient demographics and clinical characteristics, length of hospital stay, therapy, and procedures were obtained from medical records. Chi-squared tests and univariate and multivariate logistic regressions were performed. Compared to the hemodialysis departments, stay in the geriatric departments, ICUs, and haemato-oncology departments increased the risk for VRE colonization 7.6, 5.4, and 5.5 times, respectively. Compared to inpatients who were hospitalized 48 h before stool sampling for VRE isolation, inpatients hospitalized 3–7, 8–15, and longer than 16 days before sampling had 5.0-, 4.7-, and 6.6-fold higher risk for VRE colonization, respectively. The use of cephalosporins and fluoroquinolones increased the risk for VRE colonization by 2.2 and 1.9 times, respectively. The age ≥ 65 years increased the risk for VRE colonization 2.3 times. In comparison to the University Clinical Centre of Serbia, the hospital stays at Zemun and Zvezdara University Medical Centres were identified as a protector factors. The obtained results could be valuable in predicting the fecal VRE colonization status at patient admission and consequent implementation of infection control measures targeting at-risk inpatients where VRE screening is not routinely performed.
SER-109: An Oral Investigational Microbiome Therapeutic for Patients with Recurrent Clostridioides difficile Infection (rCDI)
Authors: Khanna Sahil , Sims Matthew , Louie Thomas J. , Fischer Monika , LaPlante Kerry , Allegretti Jessica , Hasson Brooke R. , Fonte Allyson T. , McChalicher Christopher , Ege David S. , Bryant Jessica A. , Straub Timothy J. , Ford Christopher B. , Henn Matthew R. , Wang Elaine E. L. , von Moltke Lisa , Wilcox Mark H.
Published: September 2022

Clostridioides difficile infection (CDI) is classified as an urgent health threat by the Centers for Disease Control and Prevention (CDC), and affects nearly 500,000 Americans annually. Approximately 20–25% of patients with a primary infection experience a recurrence, and the risk of recurrence increases with subsequent episodes to greater than 40%. The leading risk factor for CDI is broad-spectrum antibiotics, which leads to a loss of microbial diversity and impaired colonization resistance. Current FDA-approved CDI treatment strategies target toxin or toxin-producing bacteria, but do not address microbiome disruption, which is key to the pathogenesis of recurrent CDI. Fecal microbiota transplantation (FMT) reduces the risk of recurrent CDI through the restoration of microbial diversity. However, FDA safety alerts describing hospitalizations and deaths related to pathogen transmission have raised safety concerns with the use of unregulated and unstandardized donor-derived products. SER-109 is an investigational oral microbiome therapeutic composed of purified spore-forming Firmicutes. SER-109 was superior to a placebo in reducing CDI recurrence at Week 8 (12% vs. 40%, respectively; p < 0.001) in adults with a history of recurrent CDI with a favorable observed safety profile. Here, we discuss the role of the microbiome in CDI pathogenesis and the clinical development of SER-109, including its rigorous manufacturing process, which mitigates the risk of pathogen transmission. Additionally, we discuss compositional and functional changes in the gastrointestinal microbiome in patients with recurrent CDI following treatment with SER-109 that are critical to a sustained clinical response.
Derivation of a Precise and Consistent Timeline for Antibiotic Development
Authors: Stennett Henry L. , Back Catherine R. , Race Paul R.
Published: September 2022

Antibiotic resistance is a global health crisis. New classes of antibiotics that can treat drug-resistant infections are urgently needed. To communicate this message, researchers have used antibiotic development timelines, but these are often contradictory or imprecise. We conducted a systematic literature review to produce an antibiotic timeline that incorporates the dates of discovery, first use, and initial reports of the emergence of resistance for the 38 classes of clinically used antibiotics. From our timeline, we derive lessons for identifying new antibiotics that are less prone to resistance. These include a required focus on molecules that exhibit multiple modes of action, possess unusually long ‘resistance windows’, or those that engage cellular targets whose molecular architectures are at least in part decoupled from evolutionary pressures. Our analysis also further highlights the importance of safeguarding antibiotics as a mechanism for mitigating the development of resistance. We have made our data and sources freely available so that the research community can adapt them to their own needs.
Genome-Wide Identification and Characterization of Chinese Cabbage S1fa Transcription Factors and Their Roles in Response to Salt Stress
Authors: Anwar Ali , Zhang Shu , Wang Li-Xia , Wang Fengde , He Lilong , Gao Jianwei
Published: September 2022

The S1fa transcription factor is part of a small family involved in plant growth and development and abiotic stress tolerance. However, the roles of the S1fa genes in abiotic stress tolerance in Chinese cabbage are still unclear. In this study, four S1fa genes in the Chinese cabbage genome were identified and characterized for abiotic stress tolerance. Tissue-specific expression analysis suggested that three of these four S1fa genes were expressed in all tissues of Chinese cabbage, while Bra006994 was only expressed in the silique. Under Hg and Cd stresses, the S1fa genes were significantly expressed but were downregulated under NaCl stresses. The Bra034084 and Bra029784 overexpressing yeast cells exhibited high sensitivity to NaCl stresses, which led to slower growth compared with the wild type yeast cells (EV) under 1 M NaCl stress. In addition, the growth curve of the Bra034084 and Bra029784 overexpressing cells shows that the optical density was reduced significantly under salt stresses. The activities of the antioxidant enzymes, SOD, POD and CAT, were decreased, and the MDA, H2O2 and O2− contents were increased under salt stresses. The expression levels of cell wall biosynthesis genes Ccw14p, Cha1p, Cwp2p, Sed1p, Rlm1p, Rom2p, Mkk1p, Hsp12p, Mkk2p, Sdp1p and YLR194c were significantly enhanced, while Bck1p, and Ptc1p were downregulated under salt stresses. These results suggest that the Bra034084 and Bra029784 genes regulate cell wall biosynthesis and the defense regulatory system under salt stresses. These findings provide a fundamental basis for the further investigation of crop genetic modification to improve crop production and abiotic stress tolerance in Chinese cabbage.
Exogenous Betaine Enhances the Protrusion Vigor of Rice Seeds under Heat Stress by Regulating Plant Hormone Signal Transduction and Its Interaction Network
Authors: Mo Xu , Qian Jingya , Liu Peng , Zeng Hongli , Chen Guanghui , Wang Yue
Published: September 2022

Rice is an important food crop. Rice seedlings are mainly composed of root, coleoptile, mesocotyl and euphylla. The elongation of coleoptile and mesocotyl promotes the emergence of rice seedlings. Therefore, analyzing the mechanism of coleoptile and mesocotyl elongation is important for the cultivation of rice varieties. Due to global warming, heat stress is threatening rice yields. Betaine plays an important role in plant resistance to heat stress; however, we lack research on its regulation mechanism of rice seed germination under heat stress. Therefore, we explored the effects of soaking seeds with betaine at different concentrations on rice seed germination under heat stress. According to the results, soaking seeds with 10 mM of betaine could effectively improve the seeds’ germination potential and rate under heat stress to promote the germination of rice seeds. To clarify the mitigation mechanism of betaine in heat stress, we measured the antioxidant enzyme activity, malondialdehyde content, soluble protein content and endogenous hormone content of seed protrusion under heat stress. We constructed the cDNA library for transcriptome sequencing. According to the results, 10 mM of betaine improved the activities of the superoxide dismutase, peroxidase and catalase of seed protrusion under heat stress to reduce the malondialdehyde content and increase the soluble protein content to alleviate the effect of heat stress on rice seed germination. The detection of the endogenous hormone content showed that soaking seeds with 10 mM of betaine increased the content of gibberellin and decreased the contents of auxin and abscisic acid of seed protrusion under heat stress. According to the transcriptome analysis, betaine can induce the expressions of key genes in the biosynthesis and metabolism of auxin, abscisic acid and gibberellins in the seed coleoptile and mesocotyl elongation stage, regulate the signal transduction of three hormones and promote the germination of rice seeds under heat stress. This study revealed, for the first time, the physiological and molecular regulation mechanism of betaine promotion of seed germination under heat stress.
Nutritional Quality and Safety Characteristics of Imported Biscuits Marketed in Basrah, Iraq
Authors: Haider Noor N. , Altemimi Ammar B. , George Saher S. , Baioumy Ahmed Adel , El-Maksoud Ahmed Ali Abd , Pasqualone Antonella , Abedelmaksoud Tarek Gamal
Published: September 2022

The ingredients and the preparation methods influence biscuit quality and safety. In Iraq, biscuit imports are increasing every year, but no information is available in the scientific literature on their quality and safety features. This work analyzed three types of biscuits (cookies, crackers, and digestives) sampled in the Basrah markets (Iraq) but produced in Spain, Iran, Turkey, and United Arab Emirates. Nine different brands were considered for each country of origin (n = 36), with three replicates per sample. Moisture, ash, fat, proteins, fiber, water activity, peroxide value, 5-hydroxymethyl-2-furfural (HMF), acrylamide, heavy metals, and microbial load were analyzed. All the nutritional parameters were significantly influenced by the variables “Biscuit type” and “Country”. Cookies showed significantly higher fat content and lower protein content than crackers and digestives, as well as higher peroxide value (which was below the limit set by the FAO/WHO within the World Wood Program). Spanish samples had more fat and fewer proteins than biscuits made in other countries. Very high variability was observed in HMF (from not detected to 62.08 mg/kg) and AA content (reaching 1421.8 μg/kg). Cadmium was always absent, and lead was considerably below the allowed limit. Yeasts and molds were above the limits in five samples.
Combining Gamification and Augmented Reality to Raise Interest in Logistics Careers
Authors: Putz-Egger Lisa-Maria , Beil Denise , Dopler Silvia , Diephuis Jeremiah
Published: September 2022

The logistics and transport industry is currently facing the major challenge of having a global shortage of skilled workers. To address this challenge, this paper evaluates the application of gamification in combination with augmented reality (AR) as a new approach to attract the interest of people of all ages to the logistics sector. The aim of the paper is to determine whether a gamified AR-based application called Logistify is a feasible approach to make logistics jobs more attractive. We used a qualitative approach in three phases by collecting and analysing data from different perspectives of players, teachers, instructors, and programmers about the application: (1) analysing game characteristics with programmers and workshops instructors, (2) collecting feedback from players and teachers, and (3) evaluating game scores. The evaluation shows that gamification in combination with augmented reality is a promising tool to attract people to the logistics sector and to change their perception of logistics professions. It can be concluded that the gamified AR approach is capable of increasing interest in jobs in a particular sector.
Periodontal Disease Diagnosis in the Context of Oral Rehabilitation Approaches
Authors: Checherita Laura Elisabeta , Antohe Magda Ecaterina , Stamatin Ovidiu , Rudnic Ioana , Lupu Iulian Costin , Croitoru Irina , Surdu Amelia , Cioloca Daniel , Gradinaru Irina , Francu Laurian , Foia Iolanda , Vascu Bogdan Mihai , Fătu Ana Maria
Published: September 2022

Periodontal diseases generally correspond to a disturbance in the balance between the host’s defense and the micro-organisms colonizing the periodontal environment. The exact mechanisms underlying the destruction of the periodontium remain to be fully elucidated. Our study aims to quantify the main bacteria pool involved in periodontal pathology and associate it with other factors involved in the onset of periodontal disease so that an accurate diagnosis with profound implications for the therapeutic algorithm can be developed. Micro-Ident tests, based on the polymerase chain reaction (PCR) technique, were used for the study group, chosen for their high specificity in identifying periodontopathogenic bacteria and determining their relative numbers. The results of our study indicate an increased concentration of 4.50 (number of strains) for Capnocytophaga, followed by Tannerella forsythia, in a concentration of 3.50; the next highest concentration percentages are for Treponemei denticola, and Prevotela intermedia, low concentrations were found for Fusobacterium nucleatum and Porphyromonas. The concentration of each type of bacteria is reflected in the clinical picture and constitutes the starting point for a targeted antibiotic therapy. Following the effects of antibiotic-targeted therapy obtained from the evaluation of the micro-IDent B test results on the periodontium of the supporting teeth, we observed that the values of the periodontal indices change slightly at 3-month intervals with a predominance of plaque, bleeding, and gingival indices, and less in the indices concerning the depth of the probing pocket and the loss of attachment on the buccal and oral surfaces. In conclusion, our study emphasizes a direct relationship between the subgingival tartar presence and the patients age, gingival recession, presence of periodontal pockets, dental mobility, as well as the periodontal indexes: plaque index, bleeding index, and gingival index. The correlation of negative values of periodontal indices with the nature of the involved bacteria materializes in relevant starting points in the elaboration of the periodontal diagnosis of the therapeutic plan and predictability of the prognosis of oral rehabilitation.
The Antioxidant Potential of Ficus Umbellata Vahl (Moraceae) That Accelerates In Vitro and the In Vivo Anti-Inflammatory Protective Effects
Authors: Silihe Kevine Kamga , Zingue Stephane , Kemegne Sipping Marius Trésor , Cazanevscaia Anna Busuioc , Dediu Botezatu Andreea Veronica , Njamen Dieudonne , Dinica Rodica Mihaela
Published: September 2022

Ficus umbellata Vahl (Moraceae), is a plant with health benefits involved in the management of menopause physiological disorders and cancers. This study aimed at investigating the antioxidant and anti-inflammatory activities of aqueous (FUAq) and methanolic (FUMeOH) extracts of Ficus umbellata. Their antioxidant activities were assayed by free radical scavenging using DPPH and ABTS assays, total antioxidant capacity, and ferrous reducing power (FRAP). Further, the effects of FUAq and FUMeOH on murine erythrocyte membrane hemolysis and protein denaturation were investigated. The in vivo anti-inflammatory activity was determined in Wistar rats with carrageenan-induced paw oedema. At tested concentrations, FUAq and FUMeOH demonstrated strong radical scavenging that was dose- and time-dependent, as well as total antioxidant capacity and ferrous ions reducing power. Moreover, they were able to stabilize murine red blood cell membranes against heat induced hemolysis and inhibit the denaturation of egg albumin at concentrations ranging from 0.125–2 mg/mL. Ficus umbellata methanolic extract at doses of 50 and 200 mg/kg endow antiedematous properties with edema inhibition percentages of 71.16 ± 1.72% and 72.98 ± 7.51%, respectively. Our findings shed light on the antioxidant and anti-inflammatory properties of Ficus umbellata that could be used in novel and safe strategies to overwhelm oxidative and inflammatory related diseases.
Towards Urban Sustainability: Developing Noise Prediction Model in an Informal Setting
Authors: Mohammed Murtala Uba , Badamasi Murtala M. , Usman Fahad , Zango Zakariyya Uba , Dennis John Ojur , Aljameel Abdul’aziz I. , Mohammed Ali Mohammed Khalil , Aldaghri Osamah A. , Ibnaouf Khalid Hassan , Hussein Tasneem Mohammed
Published: September 2022

Noise remains an important challenge, particularly in informal settings where planning and regulation are relatively weak. This study aims at developing a model to predict noise in a largely informal urban Kano, the second most populated city in Nigeria. Sound level meter (SLM) 200 TL was used to measure noise at locations covering different land use: residential, industrial, commercial, educational, and administrative areas. Data were collected for seven days, and each day measurements were taken for six hours: 8–10 a.m., 12–2 p.m. and 4–6 p.m. Land use, population density, residential division, traffic volume, and land cover were used to generate a noise model using weighted geographic regression. The findings revealed that noise in the area is higher than the permissible limits set by the WHO and Nigeria’s regulatory agency. The model identified population density as the most influencing factor, followed by land cover, traffic volume and distance to the road, then land use. Seventy three percent of the model’s residual are below five, indicating a significant association between noise and the variables used. The R2 ranges between 18% and 26% depending on the time of the day. Noise in the area can be effectively control by paying serious attention to city planning and enforcing traffic regulation measures.
Effect of Zinc Aluminum Magnesium Coating on Spot-Welding Joint Properties of HC340LAD + ZM Steel
Authors: Chen Xiang , Peng Xinjie , Yuan Xinjian , Xiong Ziliu , Lu Yue , Lu Shenghai , Peng Jian
Published: September 2022

Zn-Al-Mg (zinc, aluminum and magnesium)-coated steel is gradually replacing traditional hot-dip galvanized steel due to its excellent corrosion resistance, self-healing properties and good surface hardness. However, the effect of Zn-Al-Mg coating on the resistance spot-welding joint properties of HC340LAD + ZM steel plates is not clear, and there are few systematic studies on it. In this paper, L16 (43) orthogonal experiments were designed on Zn-Al-Mg-coated steel HC340LAD + ZM (thickness = 1 mm). In addition, taking the tensile shear force as the main evaluation standard, the optimal spot-welding process properties could be achieved when the welding current, the welding time and the electrode loading were 10 kA, 14 cycles and 2.6 kN, respectively. On this basis, the formation mechanism, microstructure and corrosion properties of two plates of steels, with or without zinc, aluminum and magnesium coating under different welding times, were studied. The presence of Zn-Al-Mg coating slightly affected the mechanical properties of welding joints. However, the corrosion current of the body material containing Zn-Al-Mg plating was 7.17 times that of the uncoated plate.