bluuebunny/crossref_metadata_2025 huggingface dataset:
Temperature-dependent spawning behaviour and larval thermohaline associations of Bering Sea groundfish
Authors: Vary L , Rogers L , Harte M , Howard R , Ciannelli L
Published: July 2023

The Bering Sea is a productive large marine ecosystem that supports numerous commercial fisheries, while climate change is introducing rapid warming and freshening, especially in coastal water. The success of early life stages of marine fish can impact adult abundance levels; little is known about how behavioural or physiological plasticity in relation to environmental changes at one stage (e.g. spawning) may affect survival during subsequent stages (e.g. larvae), nor whether trade-offs exist that affect how a species demonstrates such plasticity. We utilized a statistical approach to examine phenological and geographical flexibility in spawning behaviour for four species of groundfish. In situ sea surface temperature (SST) and salinity (SSS) associations were also estimated by statistical models for six species of groundfish larvae. All species exhibited greater spawning geography flexibility than phenological flexibility during the egg stage. All larval stages exhibited specific temperature and salinity associations across unique combinations of SST and SSS. These species-specific patterns, in the context of potential climate change impacts, suggest that flexibility in spawning behaviour may not adequately compensate for the presence of unfavourable habitats at the larval stage.
Parallel And Divergent Morphological Adaptations Underlying The Evolution of Jumping Ability in Ants
Authors: Aibekova L , Keller R A , Katzke J , Allman D M , Hita-Garcia F , Labonte D , Narendra A , Economo E P
Published: July 2023

Synopsis

Jumping is a rapid locomotory mode widespread in terrestrial organisms. However, it is a rare specialization in ants. Forward jumping has been reported within four distantly related ant genera: Gigantiops, Harpegnathos, Myrmecia, and Odontomachus. The temporal engagement of legs/body parts during jump, however, varies across these genera. It is unknown what morphological adaptations underlie such behaviors and whether jumping in ants is solely driven directly by muscle contraction or additionally relies on elastic recoil mechanism. We investigated the morphological adaptations for jumping behavior by comparing differences in the locomotory musculature between jumping and non-jumping relatives using X-ray micro-CT and 3D morphometrics. We found that the size-specific volumes of the trochanter depressor muscle (scm6) of the middle and hind legs are 3–5 times larger in jumping ants, and that one coxal remotor muscle (scm2) is reduced in volume in the middle and/or hind legs. Notably, the enlargement in the volume of other muscle groups is directly linked to the legs or body parts engaged during the jump. Furthermore, a direct comparison of the muscle architecture revealed two significant differences between jumping vs. non-jumping ants: First, the relative Physiological Cross-Sectional Area (PCSA) of the trochanter depressor muscles of all three legs were larger in jumping ants, except in the front legs of Odontomachus rixosus and Myrmecia nigrocincta; second, the relative muscle fiber length was shorter in jumping ants compared to non-jumping counterparts, except in the front legs of O. rixosus and M. nigrocincta. These results suggest that the difference in relative muscle volume in jumping ants is largely invested in the area (PCSA), and not in fiber length. There was no clear difference in the pennation angle between jumping and non-jumping ants. Additionally, we report that the hind leg length relative to body length was longer in jumping ants. Based on direct comparison of the observed vs. possible work and power output during jumps, we surmise that direct muscle contractions suffice to explain jumping performance in three species, except for O. rixosus, where the lack of data on jumping performance prevents us from drawing definitive conclusions for this particular species. We suggest that increased investment in jumping-relevant musculature is a primary morphological adaptation that separates jumping from non-jumping ants. These results elucidate the common and idiosyncratic morphological changes underlying this rare adaptation in ants.

まとぅみ (Okinawan language—Uchinaaguchi)

要旨 (Japanese)

РЕЗЮМЕ (Kazakh)

ZUSAMMENFASSUNG (German)
Diagnostic and antibiotic stewardship lessons: an outpatient assessment of symptomatic reflex urinalysis ordering accuracy using an electronic best-practice alert
Authors: Nguyen Hien M , Flerchinger Shaun , Smith Jeffrey R , Felcher Andrew H , Turley Marianne , Mcnamara Michael
Published: July 2023

Background

It is not well known how reliably clinicians order reflex urinalysis to microscopy and culture (rUA-cx) for outpatient urinary tract infection (UTI) workup. Antibiotic appropriateness cannot be fully appreciated until the prevalence of UTIs and asymptomatic bacteriuria (ASB) are realized.

Objective

This quality improvement study has two major aims, first to determine UTI symptom accuracy for rUA-cx ordering and second, to confirm UTI and ASB cases by integrating rUA-cx and cascaded urinalysis results. Antibiotic utilization and diagnostic coding were secondarily linked to UTIs and ASB.

Methods

An electronic best-practice alert informed the ordering of two rUA-cx options: symptomatic- rUA-cx specifically for dysuria, frequency, urgency, costovertebral pain, suprapubic pain or fever versus non-specific-rUA-cx for vague complaints. UTI symptoms were verified by chart review. Confirmed UTI was defined as a significant culture with UTI symptoms and ASB as a significant culture without UTI symptoms.

Results

rUA-cx (2065) were prospectively collected over 6 months from female patients at risk for uncomplicated UTIs. Symptomatic-rUA-cx and non-specific-rUA-cx were associated with UTI symptoms for 53% (809/1527) and 20% (107/538), respectively. Overall, 44% (916/2065) of all rUA-cx had UTI symptoms. rUA-cx were overordered by a factor of 9 (2065/225) for every confirmed UTI. The UTI-to-ASB relative ratio was 2.6 (225/86). Regarding UTI-relevant antibiotics, 39% (214/553) were appropriately associated with UTI whereas only 22% (74/339) of inappropriate antibiotics were captured by the ASB definition, underestimating the problem 4-fold.

Conclusions

UTI and ASB remain challenging to categorize despite a meticulous method that applied acceptable criteria.
Structural insights into endothelin receptor signalling
Authors: Shihoya Wataru , Sano Fumiya K , Nureki Osamu
Published: July 2023

Endothelins and their receptors, type A (ETA) and type B (ETB), modulate vital cellular processes, including growth, survival, invasion and angiogenesis, through multiple G proteins. This review highlights the structural determinations of these receptors by X-ray crystallography and cryo-electron microscopy, and their activation mechanisms by endothelins. Explorations of the conformational changes upon receptor activation have provided insights into the unique G-protein coupling feature of the endothelin receptors. The review further delves into the binding modes of the clinical antagonist and the inverse agonists. These findings significantly contribute to understanding the mechanism of G-protein activation and have potential implications for drug development, particularly in the context of vasodilatory antagonists and agonists targeting the endothelin receptors.
Successful establishment, spread, and impact of the introduced parasitoid Spathius galinae (Hymenoptera: Braconidae) on emerald ash borer (Coleoptera: Buprestidae) populations in postinvasion forests in Michigan
Authors: Duan Jian J , Schmude Jonathan M , Petrice Toby R , Bauer Leah S , Poland Therese M , Chandler Jennifer L , Crandall Ryan , Elkinton Joseph S , Van Driesche Roy
Published: July 2023

Spathius galinae is a larval parasitoid native to the Russian Far East that was approved for release in the United States in 2015 for biological control of the emerald ash borer (EAB), Agrilus planipennis, an invasive beetle from Asia responsible for widespread mortality of ash trees (Fraxinus spp.) in North America. From 2015 to 2017, 1,340–1,445 females of S. galinae along with males were released into each release plot, paired with a nonrelease control plot (1–12.5 km apart), at 6 postinvasion forested sites containing abundant pole-sized ash trees in Michigan. By 2018, S. galinae had spread to all but one control plot. Based on the first year that S. galinae was found in trees in each control plot and the distances of those trees to the parasitoid release point within each site, we estimated that S. galinae spread at 3.7 (±1.9) km per year after its initial releases in 2015. The proportion of sampled trees with S. galinae broods, brood densities within sampled trees, and parasitism of EAB larvae increased sharply in both control and release plots after the last field releases in 2017, with the highest parasitism rates (42.8–60.3%) in 2020. Life table analysis showed that S. galinae alone reduced EAB’s net population growth rate by 35–55% across sites from 2018 to 2020. These results demonstrate that S. galinae has established an increasing population in Michigan and now plays a significant role in reducing EAB populations in the area.
Forced Migration and Local Public Policies: Evidence from Post-War West Germany
Authors: Chevalier Arnaud , Elsner Benjamin , Lichter Andreas , Pestel Nico
Published: July 2023

We study the effect of forced migration on public policy setting in the migrant-receiving country. After World War II, eight million expelled Germans arrived in West Germany within 5 years. We use regional variation in the population share of forced migrants across West German cities to estimate the effect of this inflow on cities’ taxation and spending decisions. To identify a causal effect, we pursue an instrumental variable strategy that leverages push factors of the expulsions while being orthogonal to local conditions in the destination regions. Our results show that cities with high inflows of forced migrants increased spending on welfare and education, decreased spending on infrastructure, raised local taxes, and incurred more debt. Part of these effects can be attributed to shifts in political preferences. The migrants held voting rights upon arrival and supported parties that explicitly catered to their interests and needs.
Towards a Shared Practice of Encampment: An Historical Investigation of UNRWA and the UNHCR to 1967
Authors: Robson Laura
Published: July 2023

This article reconsiders refugee studies’ longstanding commitment to the notion of a near-total separation between the UNHCR and UNRWA through an historical investigation of both organizations’ early approaches to refugee encampment. Without disputing the importance of the historical and institutional divisions between the two, it seeks to point out that with respect to the practice of encampment, the two agencies gradually drew nearer to each other during their first two decades of operation, as the UNHCR extended its reach into the decolonizing world and UNRWA expanded its operations across the Middle East. The parallels became particularly clear in 1967, when the ‘Protocol on the Status of Refugees’ formalized the UNHCR’s encampment-focused expansion into the decolonizing world at roughly the same time that the second Arab-Israeli war created the conditions for UNRWA to enshrine encampment as a more or less permanent institutional strategy.
Development and validation of a new quantitative reverse transcription PCR assay for the diagnosis of human sporotrichosis
Authors: Marques de Macedo Priscila , Sturny-Leclère Aude , Freitas Dayvison Francis Saraiva , Ghelfenstein-Ferreira Theo , Gutierrez-Galhardo Maria Clara , Almeida Marcos de Abreu , Rodrigues Anderson Messias , Pautet Thierry , Hamane Samia , Almeida-Paes Rodrigo , Zancopé-Oliveira Rosely Maria , Alanio Alexandre
Published: July 2023

Sporotrichosis is an emergent public health problem. The mycological diagnosis of this infection is based on culture, which is fastidious and may represent a biohazard for technicians. Although not widely implemented in routine diagnosis, molecular methodologies are fast, have good accuracy, and can be easily standardized, aiding in the early diagnosis of neglected mycoses. This study aimed at implementing a new pan-Sporothrix quantitative reverse transcription PCR (RT-qPCR) assay, and then validating it on clinical samples from confirmed human sporotrichosis cases. A total of 68 human samples with culture-confirmed diagnosis of sporotrichosis were collected from 64 patients followed at a Brazilian reference center for endemic mycoses. These samples were submitted to whole nucleic acid extraction, followed by an RT-qPCR protocol. The limit of detection was 244 fg, the efficiency was 2.0 (100%), and the assay could amplify the genetic material of the three major clinically relevant species of the genus Sporothrix. Among the 68 samples analyzed, 62 were positive in RT-qPCR, showing an overall sensitivity of 91.18%, which variated according to the type of biological sample: 96.72% in skin samples (n = 61) and 100% in respiratory samples (n = 3), whereas all cerebrospinal fluid specimens (n = 4) were negative. The specificity was 100% when tested in 25 samples from patients with other mycoses and tuberculosis. In addition, DNA from 93 fungal species did not yield positive results, confirming the high specificity of this test. Our RT-qPCR presented high sensitivity and specificity, representing an excellent tool for a fast and reliable diagnosis of human sporotrichosis.
Aspergillus sensitisation detection using point-of-care lateral flow assay in moderate to severe asthma
Authors: Wang Ran , Eades Chris , Palmer Maisie , Platt Gareth , Fowler Stephen J , Kosmidis Chris
Published: July 2023

Allergic fungal airway diseases are associated with asthma exacerbations and poor control. However, the early identification of allergic Aspergillus airway diseases can be challenging, especially in resource-poor countries. We aimed to evaluate the clinical utility of the point-of-care Aspergillus IgG–IgM lateral flow assay in diagnosing Aspergillus airway diseases in patients with moderate–severe asthma. Patients with moderate–severe asthma, severe asthma with fungal sensitisation (SAFS) and allergic bronchopulmonary aspergillosis (ABPA) were recruited. Clinical information was extracted from clinical records. Blood samples were collected for serological tests. Serum samples were evaluated using Aspergillus immunochromatographic test (ICT). A total of 65 patients were recruited into the study, of whom 23.1% had clinical diagnosis of ABPA, 18.5% had SAFS and 58.5% had moderate-to-severe asthma who did not fit ABPA or SAFS criteria. The ICT test gave a sensitivity of 69 [95% confidence interval: 51–88]% and a specificity of 77 [60–88]% in predicting a positive Aspergillus IgG test. The sensitivity and specificity for a positive Aspergillus IgE were 77 [59–88]% and 86 [71–94]%, respectively. The majority (sensitivity: 87 [62–96]%) of patients with ABPA had positive ICT results, with a specificity of 70%. The negative predictive value was high (95 [82–99]%) with a low negative likelihood ratio (< 0.2), making it potentially useful in ruling out ABPA. The ICT assay may be valuable in ruling out ABPA in resource-limited countries where serological investigations are less feasible. The ICT assay may be particularly useful in ruling out ABPA and warrants further validation.
Decoding the compositions of four bright r-process-enhanced stars
Authors: Saraf Pallavi , Prieto Carlos Allende , Sivarani Thirupathi , Bandyopadhyay Avrajit , Beers Timothy C , Susmitha A
Published: July 2023

There has been a concerted effort in recent years to identify the astrophysical sites of the r-process that can operate early in the galaxy. The discovery of many r-process-enhanced (RPE) stars (especially by the R-process Alliance collaboration) has significantly accelerated this effort. However, only limited data exist on the detailed elemental abundances covering the primary neutron-capture peaks. Subtle differences in the structure of the r-process pattern, such as the relative abundances of elements in the third peak, in particular, are expected to constrain the r-process sites further. Here, we present a detailed elemental-abundance analysis of four bright RPE stars selected from the HESP–GOMPA survey. Observations were carried out with the 10-m class telescope Gran Telescopio Canarias (GTC), Spain. The high spectral signal-to-noise ratios obtained allow us to derive abundances for 20 neutron-capture elements, including the third r-process peak element osmium (Os). We detect thorium (Th) in two stars, which we use to estimate their ages. We discuss the metallicity evolution of Mg, Sr, Ba, Eu, Os, and Th in r-II and r-I stars, based on a compilation of RPE stars from the literature. The strontium (Sr) abundance trend with respect to europium (Eu) suggests the need for an additional production site for Sr (similar to several earlier studies); this requirement could be milder for yttrium (Y) and zirconium (Zr). We also show that there could be some time delay between r-II and r-I star formation, based on the Mg/Th abundance ratios.
Evidence for mass-dependent peculiar velocities in compact object binaries: towards better constraints on natal kicks
Authors: Zhao Yue , Gandhi Poshak , Dashwood Brown Cordelia , Knigge Christian , Charles Phil A , Maccarone Thomas J , Nuchvanichakul Pornisara
Published: July 2023

We compile a catalogue of low-mass and high-mass X-ray binaries, some recently reported binaries that likely host a neutron star (NS) or a black hole (BH), and binary pulsars (a pulsar and a non-degenerated companion) that have measured systemic radial velocities (γ). Using Gaia and radio proper motions together with γ, we integrate their Galactic orbits and infer their post-supernova (post-SN) 3D peculiar velocities ($v_\mathrm{pec }^{z=0}$ at Galactic plane crossing); these velocities bear imprints of the natal kicks that compact objects received at birth. With the sample totalling 85 objects, we model the overall distribution of $v_\mathrm{pec }^{z=0}$ and find a two-component Maxwellian distribution with a low- ($\sigma _v \approx 21\, \mathrm{km s^{-1}}$) and a high-velocity ($\sigma _v \approx 107\, \mathrm{km s^{-1}}$) component. A further comparison between distributions of binary subgroups suggests that binaries hosting high-mass donors/luminous companions mostly have $v_\mathrm{pec }^{z=0}\lesssim 100\, \mathrm{km s^{-1}}$, while binaries with low-mass companions exhibit a broader distribution that extends up to $\sim 400\, \mathrm{km s^{-1}}$. We also find significant anticorrelations of $v_\mathrm{pec }^{z=0}$ with binary total mass (Mtot) and orbital period (Porb), at over 99 per cent confidence. Specifically, our fit suggests $v_\mathrm{pec }^{z=0}\propto M_\mathrm{tot}^{-0.5}$ and $v_\mathrm{pec }^{z=0}\propto P_\mathrm{orb}^{-0.2}$. Discussions are presented on possible interpretation of the correlations in the context of kinematics and possible biases. The sample should enable a range of follow-up studies on compact object binary kinematics and evolution.
Rotational and radio emission properties of PSR J0738−4042 over half a century
Authors: Lower M E , Johnston S , Karastergiou A , Brook P R , Bailes M , Buchner S , Deller A T , Dunn L , Flynn C , Kerr M , Manchester R N , Mandlik A , Oswald L S , Parthasarathy A , Shannon R M , Sobey C , Weltevrede P
Published: July 2023

We present a comprehensive study of the rotational and emission properties of PSR J0738−4042 using a combination of observations taken by the Deep Space Network, Hartebeesthoek, Parkes (Murriyang) and Molonglo observatories between 1972 and 2023. Our timing of the pulsar is motivated by previously reported profile/spin-down events that occurred in 2005 September and 2015 December, which result in an anomalously large braking index of n = 23 300 ± 1800. Using a Gaussian process regression framework, we develop continuous models for the evolution of the pulsar spin-down rate ($\dot{\nu }$) and profile shape. We find that the pulse profile variations are similar regardless of radio observing frequency and polarization. Small-scale differences can be ascribed to changes in the interstellar medium along the line of sight and frequency-dependent changes in magnetospheric radio emission height. No new correlated spin-down or profile events were identified in our extended data set. However, we found that the disappearance of a bright emission component in the leading edge of archival profiles between 1981 and 1988 was not associated with a substantial change in $\dot{\nu }$. This marks a notable departure from the previous profile/spin-down events in this pulsar. We discuss the challenges these observations pose for physical models and conclude that interactions between the pulsar and in-falling asteroids or a form of magnetospheric state-switching with a long periodicity are plausible explanations.
On the likelihoods of finding very metal-poor (and old) stars in the Milky Way’s disc, bulge, and halo
Authors: Sotillo-Ramos Diego , Bergemann Maria , Friske Jennifer K S , Pillepich Annalisa
Published: July 2023

Recent observational studies have uncovered a small number of very metal-poor (VMP) stars with cold kinematics in the Galactic disc and bulge. However, their origins remain enigmatic. We select a total of 138 Milky Way (MW) analogues from the TNG50 cosmological simulation based on their z = 0 properties: discy morphology, stellar mass, and local environment. In order to make more predictive statements for the MW, we further limit the spatial volume coverage of stellar populations in galaxies to that targeted by the upcoming 4MOST high-resolution survey of the Galactic disc and bulge. We find that across all galaxies, ∼20 per cent of VMP ([Fe/H] < −2) stars belong to the disc, with some analogues reaching 30 per cent. About 50 ± 10 per cent of the VMP disc stars are, on average, older than 12.5 Gyr and ∼70 ± 10 per cent come from accreted satellites. A large fraction of the VMP stars belong to the halo (∼70) and have a median age of 12 Gyr. Our results with the TNG50 cosmological simulation confirm earlier findings with simulations of fewer individual galaxies, and suggest that the stellar disc of the MW is very likely to host significant amounts of very- and extremely-metal-poor stars that, although mostly of ex situ origin, can also form in situ, reinforcing the idea of the existence of a primordial Galactic disc.
Nucleosome retention by histone chaperones and remodelers occludes pervasive DNA–protein binding
Authors: Jonas Felix , Vidavski Matan , Benuck Eli , Barkai Naama , Yaakov Gilad
Published: July 2023

DNA packaging within chromatin depends on histone chaperones and remodelers that form and position nucleosomes. Cells express multiple such chromatin regulators with overlapping in-vitro activities. Defining specific in-vivo activities requires monitoring histone dynamics during regulator depletion, which has been technically challenging. We have recently generated histone-exchange sensors in Saccharomyces cerevisiae, which we now use to define the contributions of 15 regulators to histone dynamics genome-wide. While replication-independent exchange in unperturbed cells maps to promoters, regulator depletions primarily affected gene bodies. Depletion of Spt6, Spt16 or Chd1 sharply increased nucleosome replacement sequentially at the beginning, middle or end of highly expressed gene bodies. They further triggered re-localization of chaperones to affected gene body regions, which compensated for nucleosome loss during transcription complex passage, but concurred with extensive TF binding in gene bodies. We provide a unified quantitative screen highlighting regulator roles in retaining nucleosome binding during transcription and preserving genomic packaging.
A comprehensive review and meta-regression analysis of randomized controlled trials examining the impact of vitamin B12 supplementation on homocysteine levels
Authors: Sohouli Mohammad Hassan , Almuqayyid Faisal , Alfardous Alazm Aya , Ziamanesh Fateme , Izze da Silva Magalhães Elma , Bagheri Seyedeh Elaheh , Rodrigues de Oliveira Bianca , Alfardous Alazm Mahmoud , Adi Abdul Rahman , Alomar Sara , Abu-Zaid Ahmed
Published: July 2023

Context

Although some research suggests that vitamin B12 (hereafter, B12) supplements can lower homocysteine (Hcy) levels and treat hyperhomocysteinemia, these results are still ambiguous when B12 is taken as an isolated supplement.

Objective

This study sought to determine how existing randomized controlled trials (RCTs) could be used to examine the effects of B12 supplementation on Hcy.

Data Sources

To find pertinent RCTs up to June 2022, databases, including PubMed/Medline, Web of Science, Scopus, Cochrane Library, and Embase, were searched.

Data Extraction

All selected RCTs investigated the impact of B12 supplements on Hcy. A meta-analysis of the eligible studies was performed using the random-effects model.

Data Analysis

This review included a total of 21 RCTs (N = 1625 participants). Hcy levels were significantly lower after B12 supplementation compared with the control group (pooled weighted mean difference, –4.15 μmol/L; 95% confidence interval, –4.86, –3.45; P < 0.001), and this reduction was even greater with intervention durations ≥12 weeks and doses >500 µg/d. Furthermore, the effect of B12 supplementation in the form of hydroxocobalamin on the reduction of Hcy level was greater compared with other forms.

Conclusion

In conclusion, this meta-analysis shows that B12 supplementation has a positive impact on lowering blood Hcy levels, particularly when administered for a longer period and at a larger dose.

Systematic Review Registration

PROSPERO registration no. CRD42022364066
The psychological basis of hunger and its dysfunctions
Authors: Stevenson Richard J
Published: July 2023

This article describes a new and emerging psychological perspective on hunger, together with the implications of that perspective, which is based upon learning and memory. Hunger is a psychological state characterized by a desire to eat. Historically, conceptions of hunger have largely been expressed in terms of physiology (eg, biological process X causes hunger). However, physiology neither offers a psychological account of hunger nor explains why memory impairment can eliminate hunger. Two forms of hunger are identified – specific and general. Specific hunger is for particular palatable foods. It involves recollecting episodic memories of eating that food, when an associated cue is encountered (eg, an advert). General hunger is a desire to eat triggered by temporal (eg, it is lunchtime) or interoceptive (eg, tummy rumble) cues. It involves semantic memory retrieval, which then augments the expected – remembered – pleasure for any food. Both hungers are supported by the medial temporal lobe memory system. Damage to this system can occur from eating a Western-style diet and, longer-term, from obesity and its consequences. Medial temporal lobe memory damage may cause deficits in specific hunger, but most especially in general hunger, resulting in little motivation to eat foods that the individual considers to be of low-to-moderate palatability, such as fruit and vegetables. The implications of this account for teaching people hunger, for how hunger is affected by diet, for public education, and pharmaceutical intervention, are discussed. Psychological concepts of hunger are widely used in nutritional practice. This article provides a new and emerging perspective on the psychological basis of hunger and its implications.
Activation of Strigolactone Biosynthesis by the DWARF14-LIKE/KARRIKIN-INSENSITIVE2 Pathway in Mycorrhizal Angiosperms, but Not in Arabidopsis, a Non-mycorrhizal Plant
Authors: Mashiguchi Kiyoshi , Morita Ryo , Tanaka Kai , Kodama Kyoichi , Kameoka Hiromu , Kyozuka Junko , Seto Yoshiya , Yamaguchi Shinjiro
Published: July 2023

Strigolactones (SLs) are a class of plant hormones that regulate many aspects of plant growth and development. SLs also improve symbiosis with arbuscular mycorrhizal fungi (AMF) in the rhizosphere. Recent studies have shown that the DWARF14-LIKE (D14L)/KARRIKIN-INSENSITIVE2 (KAI2) family, paralogs of the SL receptor D14, are required for AMF colonization in several flowering plants, including rice. In this study, we found that (−)-GR5, a 2′S-configured enantiomer of a synthetic SL analog (+)-GR5, significantly activated SL biosynthesis in rice roots via D14L. This result is consistent with a recent report, showing that the D14L pathway positively regulates SL biosynthesis in rice. In fact, the SL levels tended to be lower in the roots of the d14l mutant under both inorganic nutrient-deficient and -sufficient conditions. We also show that the increase in SL levels by (−)-GR5 was observed in other mycorrhizal plant species. In contrast, the KAI2 pathway did not upregulate the SL level and the expression of SL biosynthetic genes in Arabidopsis, a non-mycorrhizal plant. We also examined whether the KAI2 pathway enhances SL biosynthesis in the liverwort Marchantia paleacea, where SL functions as a rhizosphere signaling molecule for AMF. However, the SL level and SL biosynthetic genes were not positively regulated by the KAI2 pathway. These results imply that the activation of SL biosynthesis by the D14L/KAI2 pathway has been evolutionarily acquired after the divergence of bryophytes to efficiently promote symbiosis with AMF, although we cannot exclude the possibility that liverworts have specifically lost this regulatory system.
Trends in cause-specific mortality: deaths of despair in Spain, 1980–2019
Authors: Piñeiro Bárbara , Spijker Jeroen J A , Trias-Llimós Sergi , Blanes Llorens Amand , Permanyer Iñaki
Published: July 2023

Background

Research from various countries has shown increases in alcohol- and drug-related deaths and suicide, known as ‘deaths of despair’ over recent decades, particularly among low-educated middle-aged individuals. However, little is known about trends in death-of-despair causes in Spain. Therefore, we aim to descriptively examine this among 25–64-year-olds from 1980 to 2019 and by educational attainment for the years 2017–19.

Methods

We obtained mortality and population data from the National Institute of Statistics to estimate age-standardized mortality rates and assess educational inequalities using the relative index of inequality (RII).

Results

Deaths of despair as a share of total mortality slightly increased from 2000 onwards, particularly among 25–64-year-old men (from 9 to 10%). Only alcohol-related mortality declined relatively more since 1980 compared with all-cause mortality. Regarding educational differences, low-educated men presented higher mortality rates in all death-of-despair causes (alcohol-related: RII 3.54 (95% CI: 2.21–5.66); drug-related: RII 3.49 (95% CI: 1.80–6.77); suicide: RII 1.97 (95% CI: 1.49–2.61)). Women noteworthy differences were only observed for alcohol-related (RII 3.50 (95% CI: 2.13–5.75)).

Conclusions

Findings suggest an increasing proportion of deaths of despair among 25–64-year-olds since 2000, particularly among men. Public health policies are needed to reduce and prevent these premature and preventable causes of mortality.
Patient skin dose measurement and risk of deterministic effect during fluoroscopy cardiac procedures
Authors: Nana Ruth Njantang , Inkoom Stephen , Moyo Maurice Ndontchueng , Mvoufo Fokou , Hasford Francis , Owusu-Manteaw Philip , Motapon Ousmanou
Published: July 2023

This study aimed at assessing patient’s peak skin doses (PSD) during fluoroscopy cardiac procedures and proposed a look up table to enhance patient’s dose management. Perspex phantom and thermoluminescent dosemeters (TLD) were irradiated for different dose levels with X-ray equipment (Philips Azurion 7). It was found that PSD measures were higher than the kerma at the interventional reference point [K (IRP)] reported with factors 1.55, 1.75 and 2.88 for anterior posterior (AP0o), left anterior oblique (LAO45o) and right anterior oblique (RAO45o), respectively. The equations describing the correlation between the PSD measured kerma area product and cumulative air kerma were found with R-square values of 0.98 and 0.99, respectively. The statistical analysis shows a strong linear correlation between PSD and K (IRP) (P-value = 0.05). It was also found that 27% of the patients population considered in this work, received a skin dose higher than the threshold of deterministic effect of 2 Gy and a look up table with the equation of fitness were proposed to be implemented in the facility for K (IRP) higher than 500 mGy.
Midfrontal theta as an index of conflict strength in approach–approach vs avoidance–avoidance conflicts
Authors: Levy Ariel , Enisman Maya , Perry Anat , Kleiman Tali
Published: July 2023

The seminal theory of motivational conflicts distinguishes between approach–approach (AP-AP) conflicts, in which a decision is made between desirable alternatives, and avoidance–avoidance (AV-AV) conflicts, in which a decision is made between undesirable alternatives. The behavioral differences between AP-AP and AV-AV conflicts are well documented: abundant research showed that AV-AV conflicts are more difficult to resolve than AP-AP ones. However, there is little to no research looking into the neural underpinnings of the differences between the two conflict types. Here, we show that midfrontal theta, an established neural marker of conflict, distinguished between the two conflict types such that midfrontal theta power was higher in AV-AV conflicts than in AP-AP conflicts. We further demonstrate that higher midfrontal theta power was associated with shorter decision times on a single-trial basis, indicating that midfrontal theta played a role in promoting successful controlled behavior. Taken together, our results show that AP-AP and AV-AV conflicts are distinguishable on the neural level. The implications of these results go beyond motivational conflicts, as they establish midfrontal theta as a measure of the continuous degree of conflict in subjective decisions.
Determining the phosphorus release curve for Smizyme TS G5 2,500 phytase from 500 to 2,500 FTU/kg in nursery pig diets
Authors: Gaffield Katelyn N , Williams Hadley R , Becker Larissa L , DeRouchey Joel M , Woodworth Jason C , Tokach Mike D , Goodband Robert D , Gebhardt Jordan T , Faser Jill M
Published: July 2023

A total of 320 pigs (Line 241 × 600, DNA, Columbus, NE; initially 11.9 ± 0.22 kg) were used in a 21-d growth study to determine the available P (aP) release curve for Smizyme TS G5 2,500 (Barentz, Woodbury, MN). At approximately 19 d of age, pigs were weaned, randomly allotted to pens, and fed common starter diets. Pigs were blocked by average pen body weight (BW) and randomly allotted to one of eight dietary treatments on day 18 postweaning, considered day 0 of the study. Dietary treatments were derived from a single basal diet and ingredients including phytase, monocalcium P, limestone, and sand were added to create the treatment diets. Treatments included three diets containing increasing inorganic P from monocalcium P (0.11%, 0.20%, and 0.28% aP), or five diets with increasing phytase (500, 1,000, 1,500, 2,000, or 2,500 FTU/kg) added to the diet containing 0.11% aP. All diets were corn–soybean meal–canola meal-based and were formulated to contain 1.24% standardized ileal digestibility Lys, 0.30% phytate P, and an analyzed Ca:P ratio of 1.10:1. Prior to the beginning of the study, all pigs were fed a diet containing 0.11% aP for a 2-d period (days 16 to 18 postweaning). At the conclusion of the study, one pig, closest to the mean weight of each pen, was euthanized and the right fibula, rib, and metacarpal were collected to determine bone ash, density, and total bone P. Bones were weighed while suspended in a vessel of water and the weights used to calculate bone density (Archimedes’ principle). For bone ash, bones were processed using the non-defatted method. For the overall experimental period, pigs fed increasing inorganic P had increased (quadratic, P ≤ 0.033) average daily gain (ADG), average daily feed intake (ADFI), and final BW and a tendency for increased (quadratic, P ≤ 0.090) gain:feed ratio (G:F). Pigs fed increasing phytase had increased (quadratic, P ≤ 0.004) ADG, G:F, and final BW and increased (linear, P = 0.019) ADFI. For fibula, rib, and metacarpal characteristics, pigs fed increasing aP from inorganic P had increased (linear, P < 0.001) bone ash weight, percentage bone ash, bone density, and bone P concentration. Additionally, pigs fed increasing phytase had increased (linear or quadratic, P < 0.05) bone ash weight, percentage bone ash, bone density, and bone P. TheaP release curve generated for Smizyme TS G5 2,500 for percentage bone ash using data generated from all three bones is aP = (0.228 × FTU/kg) ÷ (998.065 + FTU/kg).
Engaging Mature-Age Workers Through Mature-Age Practices: Examining the Roles of Focus on Opportunities and Work Centrality
Authors: Zhou Lian , Zhan Yujie , Peng Jiamin , Chen Jian
Published: July 2023

Given the global trend of labor force aging and the ongoing challenge of engaging mature-age workers, researchers have begun to explore human resource practices that are tailored to the needs of mature-age workers. However, knowledge about how such practices influence older individuals’ motivation at work is limited. Drawing upon signaling theory, we developed and examined a model that specifies why and when mature-age practices are helpful in engaging mature-age workers. Using time-lagged data from 135 Chinese workers aged 40 years or above, we found that mature-age practices are associated with mature-age workers’ focus on opportunities. Moreover, mature-age practices had a positive indirect effect on mature-age workers’ work engagement through their focus on opportunities. This positive indirect effect of mature-age practices on work engagement via focusing on opportunities was stronger for mature-age workers with lower rather than higher work centrality. The findings are discussed in terms of their theoretical implications for the aging workforce management literature and practical implications are provided for managers seeking to engage mature-age workers.
Ocular involvement in allergic drug reactions
Authors: Fernandez Edward , Phillips Elizabeth , Saeed Hajirah N.
Published: July 2023

Purpose of review

Many systemic medications have been observed to cause ocular toxicity. A subset of these reactions is thought to involve immunomodulation or a hypersensitivity reaction. As new medications are developed, ocular adverse effects are becoming increasingly prevalent. Herein we review immune-mediated drug reactions affecting they eye with special attention to the hypersensitivity mechanisms leading to ocular toxicity.

Recent findings

Recent work has focused on mechanisms and risk of immune-mediated ocular adverse drug reactions including genetic susceptibility and loss of ocular immune privilege.

Given the consequences of immune-mediated ocular adverse drug reactions, clinicians must be aware of these to facilitate early recognition and management. The prompt involvement of an ophthalmologist for diagnosis and management is often essential to preserve vision and avoid long-term morbidity.
Education via a mobile application improves health literacy after orthopaedic trauma
Authors: Brostowitz Norah J. , Heimke Isabella M. , Alejo Andrew , Vallier Heather A.
Published: July 2023

Objectives:

Health literacy is often poor among trauma patients. We introduced an educational mobile application to newly injured patients. We hypothesized that patients who utilized the app would be more informed about their injury and treatment recommendations, and that app users would demonstrate greater adherence to treatment recommendations.

Design:

Prospective, cohort study

Setting:

Level 1 trauma center

Patients/participants: Adult patients with acute orthopaedic injury

Intervention:

App with educational information about injuries, treatment, and recovery. bit.ly/traumaapp

Main Outcome Measurements:

Survey regarding knowledge about injury and treatment, and adherence to recommendations

Results:

339 patients were enrolled: 187 received information about the app; 105 of them used the app. Another 234 patients had no prior knowledge about the app. App users were more often female (56.2% vs. 37.6%, p=0.002). App users were more likely to have completed college (34.3% vs. 13.7%, p<0.0001). Better health literacy was noted among app users in all measurements, including knowledge of: fracture location (69% vs. 39%, p<0.0001), treatment (100% vs. 93%, p=0.002), weightbearing status (96% vs. 88%, p=0.001), estimated healing time (63% vs. 26%, p<0.0001), and DVT prophylaxis (93% vs. 81%, p=0.003).

Conclusions:

Patients who used the app were more likely to have accurate knowledge in all assessed categories, with over 99% reporting adherence to weightbearing recommendations. It is probable that this level of knowledge and engagement will enhance various outcomes after injury.

Level of evidence:

Prognostic Level II. See Instructions for Authors for a complete description of levels of evidence.
Soft Tissue Releases With Simultaneous Guided Growth Decrease Risk of Spastic Hip Displacement Recurrence
Authors: Hsu Cheng-Min , Sheu Huan , Lee Wei-Chun , Kao Hsuan-Kai , Yang Wen-E , Chang Chia-Hsieh
Published: July 2023

Background:

Soft tissue release (STR) is an established treatment for spastic hip displacement, but recurrence of hip displacement is not uncommon. This study aims to (1) evaluate the recurrence of hip displacement after STR, (2) define associated factors of recurrence, and (3) elucidate the effects of guided growth on hip displacement recurrence.

Methods:

The study subjects included 66 individuals with spastic cerebral palsy treated by STR with or without guided growth for hip displacement. The treatment goal was the maintenance of migration percentage (MP) to <40%. Recurrence was defined by a rebound of the MP by 5% or more after the first postoperative year. Children with recurrence were compared with those without recurrence using the Mann-Whitney U test and the χ2 test. The risk factors for recurrence were evaluated using multiple logistic regression analysis.

Results:

Nineteen individuals (29%) had a recurrence of hip displacement after the first postoperative year. They sustained a 2-fold increase in the rate of treatment failure (P < 0.001) and reoperation (P = 0.04). Age, sex, motor function, and preoperative radiographic parameters were comparable between individuals with and without recurrence. The use of guided growth was associated with less risk of recurrence than without (5% and 39%, respectively, odds ratio = 0.01 to 0.45, respectively) despite the similar risk of failure (35% and 48%, respectively, odds ratio = 0.15 to 4.82).

Conclusions:

Recurrence of the MP >5% after the first postoperative year is an important early indicator for failure to control MP to <40% and reoperation. Guided growth not only decreases coxa valga but also reduces the risk of recurrent hip displacement after STR.

Level of Evidence:

Level III; case-control study.
Clinical and Radiographic Assessment of Adult Function After Blount Disease in Childhood: An Exercise in Futility
Authors: Makarov Marina R. , Polk Jordan L. , Shivers Claire R. , Jo Chan-Hee , Rathjen Karl E. , Birch John G.
Published: July 2023

Background:

Few studies evaluate long-term function of patients with Blount disease. We sought to document patient radiographic and functional status 20 to 30 years after sugical treatment for infantile or adolescent Blount disease.

Methods:

We reviewed the medical records and radiographs of patients operated at our institution for Blount disease between 1985 and 1995. Over a 4-year period, we recruited subjects for an IRB-approved call-back study.

Results:

One hundred five patients were eligible for the call-back study. Thirty-one (30%) had a criminal record, 18 of sufficient gravity to preclude invitation to return. Of the remaining 87, 40 (46%) could not be contacted. Of 47 with valid contact information, 10 (21%) were reported as deceased (although this could not be objectively confirmed), 20 (43%) did not respond or failed to show for assessment, and 1 (2%) declined to participate. 16 subjects returned at average age 36, 22 to 31 years post-index surgery. Body mass index (BMI) averaged 45.8 (range 23.9 to 67.6). Physical Score correlated most strongly and inversely with BMI (P<0.01). Satisfaction with life correlated strongly and inversely with mechanical axis deviation (P=0.02) and radiographic osteoarthritis of the knee (P=0.02), but not BMI. There also was no correlation between severity of radiographic osteoarthritis and mechanical axis deviation (P=0.46) or BMI (P=0.52).

Conclusions:

The small fraction of patients returning for evalutation minimize clinical conclusions that can be drawn from this study, despite an intensive 4-year effort to conduct it. Management of obesity and other socioeconomic characteristics are likely the most important aspects of treatment of patients with Blount disease. Our primary conclusion is that meaningful long-term functional studies of pediatric orthopaedic conditions will not be answered by retrospective call-back studies, and must be conducted within prospective registries and regular longitudinal follow-up.

Level of Evidence:

III—Case-controlled study
Computerized Cognitive Training and 24-Month Mortality in Heart Failure
Authors: Jung Miyeon , Smith Asa B. , Giordani Bruno , Clark David G. , Gradus-Pizlo Irmina , Wierenga Kelly L. , Lake Kittie Reid , Pressler Susan J.
Published: July 2023

Background

Cognitive dysfunction predicts mortality in heart failure (HF). Computerized cognitive training (CCT) has shown preliminary efficacy in improving cognitive function. However, the relationship between CCT and mortality is unclear. Aims were to evaluate (1) long-term efficacy of CCT in reducing 24-month mortality and (2) age, HF severity, global cognition, memory, working memory, depressive symptoms, and health-related quality of life as predictors of 24-month mortality among patients with HF.

Methods

In this prospective longitudinal study, 142 patients enrolled in a 3-arm randomized controlled trial were followed for 24 months. Logistic regression was used to achieve the aims.

Results

Across 24 months, 16 patients died (CCT, 8.3%; control groups, 12.8%). Computerized cognitive training did not predict 24-month mortality (odds ratio [OR], 0.65). Older age (OR, 1.08), worse global cognition (OR, 0.73), memory (OR, 0.81), and depressive symptoms (OR, 1.10) at baseline predicted 24-month mortality.

Conclusions

Efficacious interventions are needed to improve global cognition, memory, and depressive symptoms and reduce mortality in HF.
Factors Related to Burnout Among Perinatal Home Visiting Nurses
Authors: Greene Madelyne Z. , Gillespie Kate H.
Published: July 2023

Purpose:

Risks for burnout among nurses have been understudied in public and community health settings. This qualitative descriptive study aimed to describe the experiences of nurses working in a perinatal community health program for high-risk families with a focus on challenges and risks for burnout.

Methods:

We conducted field observation and semistructured individual interviews in 2019 with nurses at 2 sites implementing Prenatal Care Coordination (PNCC), a Medicaid benefit for high-risk pregnant people and infants in Wisconsin. A larger parent study assessed the implementation of PNCC overall through a thematic analysis process. Initial deductive coding was guided by the Consolidated Framework for Implementation Research.

Findings:

Emergent themes demonstrated that PNCC nurses face several significant barriers and known risks for burnout, including role strain related to scope of practice and training, discordant racial and socioeconomic identities leading to role conflict, and low control combined with high psychological demand.

Conclusions:

Given that the COVID-19 pandemic has exacerbated burnout risk among nurses in hospital settings, and that nurses addressing social determinants of health may be at increased risk for burnout, these findings represent a critical perspective on the experiences and needs of perinatal nurses in public and community health settings.