bluuebunny/crossref_metadata_2025 huggingface dataset:
What's The Next Era Of Immune Checkpoint Inhibitors Of Renal Cell Carcinoma In Clinical Practice: An Evidence From Extended Bibliometric Analysis
Authors: Yin Zhenjie , Zhu Lifeng , You Bingyong , Sun Yingming , Zhao Yu , Bai Yuanyuan , Chen Daoxun , Chen Yongmei , Liao Shangfan , Lu Dongming , Zhang Fabiao , Wu Yongyang
Published: March 2022

Objectives: To use bibliometric analysis to articles regarding RCC treating by ICIs, extended analysis effect, and safety of enrolled paper to interpret the current situation and future trend of clinical trials. Methods: We searched in the WoSCC Database on May 30, 2021. Articles were ranked in descending order based on their total citations. Also, we investigated the uncovered trials registered in ClinicalTrials.gov to analyze the trend of the future of ICI treatment. Results: The top 30 most cited articles were identified and analyzed. The most productive year was 2020, with 12 articles published. The USA and Memorial Sloan Kettering Cancer Center were the most effective country and institutions. The 15 journals contributed to these articles, and the top five keywords were survival, therapy, nivolumab, everolimus, and immunotherapy. The 13 original articles registered on ClinicalTrials.gov: monotherapy was 38.5% and combined therapy was 61.5%. The ORR of monotherapy and combined therapy was 24.05% vs 49.03% (P< 0.001). The mPFS of monotherapy was shorter than combined therapy (4.01 mo vs 14.51 mo, P< 0.001). The incidence of grade 3/4 adverse events in single-agent was significantly lower than combined therapy (19.76% vs 59.38%, P< 0.001). 91 grey data were extracted from ClinicalTrials.gov, which included single-agent (11%) and combination therapy (89%). Conclusions: Although the side effect is unfavorable, the combined therapy will replace the single-agent ICI treatment in the future clinical trial design. The effect of treatment based on PD-1, PD-L1, and CTLA-4 has almost reached the bottleneck, the scientist should focus on new targets of checkpoints in clinical research.
Prone Positioning During Veno-Venous Or Veno-Arterial Extracorporeal Membrane Oxygenation: Feasibility And Complications After Cardiothoracic Surgery
Authors: Genty Thibaut , Cherel Quentin , Thès Jacques , Bouteau Astrid , Roman Calypso , Stéphan François
Published: March 2022

Extracorporeal membrane oxygenation (ECMO) is a standard treatment for refractory hypoxaemia (veno-venous ECMO, VV-ECMO) and cardiogenic shock (veno-arterial ECMO, VA-ECMO). Severe hypoxaemia may persist despite ECMO. Prone positioning (PP) can improve outcomes of acute respiratory distress syndrome (ARDS)(1,2). However, few data exist on PP in hypoxaemic patients receiving VV-ECMO or VA-ECMO, particularly after cardio-thoracic surgery. Here, we evaluated oxygenation and complications seen with PP during ECMO.
Development and evaluation of Recombinase Polymerase Amplification assay for diagnosis of Canine Leptospirosis
Authors: Kuppusamy senthilkumar , Nirmala K. , Tirumurugaan K.G. , Aravindbabu R.P. , Senthilkumar T.M.A. , Ravikumar G.
Published: March 2022

Canine Leptospirosis pose a public health risk in addition to life threatening disease to dogs. The molecular methods enable faster and sensitive diagnosis of leptospirosis at early stage of infection in contrast to dark field microscopy and culture. The Recombinase Polymerase Amplification (RPA) assay is a versatile alternative to polymerase chain reaction by its simple, fast, inherent enzymatic amplification of nucleic acid at constant temperature. The RPA assay to detect Leptospira DNA was optimized with Leptospira reference strains and its performance characteristic such as analytical, diagnostics and reproducibility were assessed. The limit of detection of RPA assay was estimated as 10 2 copies of genomic DNA was similar to that of PCR assay and it is specific to amplify the pathogenic Leptospira strain rather than non-pathogenic Leptospira strains and other bacterial strains. Out of 150 dog samples screened Leptospira DNA was detected in 42.6% (64/150) by RPA assay and 44.6% (67/150) by PCR. The diagnostic sensitivity and specificity of RPA assay was 92.5% (83.44% to 97.53%, 95% CI) and 97.59% (91.57% to 99.71%, 95% CI) respectively, in comparison with PCR. The RPA assay has a good diagnostic agreement with a kappa value of 0.905 (0.837 to 0.974 at 95% CI). The performance of the assay on different occasion to amplify the DNA with mean concentration of 42.24 ng/µl ± 1.44 with a coefficient of variation of 5.9% showed its good repeatability. The reproducibility assessment with the third-party testing laboratory, revealed better agreement on the performance of the assay with a kappa value of 0.81(0.547 to 1.00 at 95% CI). Since this method is simple, rapid and less expensive enables to perform at resource limited laboratories or point of care testing at field level.
Effect of MWCNTS on Spectroscopic and Thermal properties of PVB Nanocomposite films
Authors: A RAJESHWAR REDDY , N Narsimlu
Published: March 2022

In this paper, we demonstrate a simple fabrication process for polyvinyl butyral (PVB) based nanocomposite films reinforced with MWCNTS. The solution casting method was used to prepare pure PVB and composite films. XRD, FTIR, SEM and DSC methods were used to study the effect of multiwalled carbon nanotubes (MWCNTS) on the spectroscopic and thermal properties of polyvinyl butyral (PVB) composite films. The structural, spectroscopic and thermal properties of PVB based nanocomposite films were significantly altered after being reinforced with the MWCNTS. The chemical structure and crystalline phase of the composites were studied using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD), respectively. The surface morphology (SEM) was characterized using scanning electron microscopy. The XRD patterns and SEM images show the MWCNTS are well dispersed in the PVB matrix. The glass transition temperature (Tg) and melting temperature (Tm) of PVB nanocomposite films were determined using DSC analysis (from 37 ° C to 250 ° C). With increasing MWCNTS weight percentage, the glass transition temperature and the melting point of composite films have been found to enhance.
Proton Pump Inhibitors Use and Risks of Cardiovascular Disease and Mortality in Patients with Type 2 Diabetes: A Prospective Study in the UK Biobank
Authors: Geng Tingting , Chen Junxiang , Zhou Yan-Feng , Lu Qi , Wan Zhenzhen , Liu Liegang , Pan An , Liu Gang
Published: March 2022

Background: Proton pump inhibitors (PPIs) are widely used drugs for gastric-acid-related diseases, which may have an impact on the gut microbiome. We aimed to evaluate the associations of PPIs use with risks of cardiovascular disease (CVD) and all-cause mortality in patients with type 2 diabetes (T2D).Methods: We analysed the associations of PPIs use with risks of coronary artery disease (CAD), myocardial infarction (MI), heart failure (HF), stroke, and all-cause mortality in 19,229 adults with T2D using data from the UK Biobank study.Results: During a median follow-up of 10.9-11.2 years, we documented a total of 2,971 CAD, 1,827 MI, 1,192 HF, and 738 stroke cases, along with 2,297 total deaths. PPIs use was significantly associated with higher risks of CAD (HR, 1.27; 95% CI, 1.15-1.40), MI (HR, 1.34; 95% CI, 1.18-1.52), HF (HR, 1.35; 95% CI, 1.16-1.57) and all-cause mortality (HR, 1.30; 95% CI, 1.16-1.45). No significant association was observed between PPIs use and stroke (HR, 1.11; 95% CI, 0.90-1.36). The results were consistent in the subgroup analyses stratified by factors including indications of PPIs, anti-diabetic medication use, and antiplatelet drug use. Analyses in a 1:1 propensity score-matched cohort of PPIs users versus non-users yielded consistent results. Conclusion: Our data suggested that PPIs use was associated with higher risks of CVD events and mortality among patients with T2D. Prescription of PPIs among patients with T2D should be cautious, and monitoring of adverse cardiovascular events during PPIs therapy should be enhanced.
Radix Actinidiae chinensis induces autophagy in renal cell carcinoma 786-O and A498 cells by regulating PI3K/AKT/mTOR pathway
Authors: Liu Biao , Zhang Liang
Published: March 2022

Objective: Renal cell carcinoma (RCC) is a malignant tumor. This study investigated the mechanism of Radix Actinidiae chinensis (RAC) inducing autophagy in RCC cell.Methods: Cell viability and IC50 value in 786-O and A498 cells were detected by CCK8 assays. The proliferation, cell cycle, migration and invasion were detected by cloning, flow cytometry and cell invasion assays, respectively. The cell apoptosis, cycle, autophagy and related protein levels were detected by flow cytometry, AOPI assay and western blot, respectively. The number of autophagolysosomes was detected by electron microscopy. In addition, the proteins related to the PI3K/AKT/mTOR pathway were detected by western blot.Results: IC50 values of RAC in 786-O and A498 cells were 14.76 mg/mL and 13.09 mg/mL, respectively. RAC treatment reduced cell viability, proliferation, migration and invasion, blocked the S phase cells, and promoted apoptosis and autophagyin 786-O and A498 cells. It also increased the autophagy-related proteins LC3II/I and Beclin-1, and decreased the level of p62. In addition, RAC activated the levels of apoptosis-related proteins and reduce the levels of PI3K/AKT/mTOR pathway-related proteins. However, the addition of autophagy inhibitor 3-MA attenuated the effect of RAC on apoptosis, autophagy and the expression of related proteins in RCC cells.Conclusion: RAC can induce autophagy in RCC by blocking PI3K/AKT/mTOR pathway, to inhibit the progression of RCC cells.
Transdermal nanolipoplex simultaneously inhibits subcutaneous melanoma growth and suppresses systemically metastatic melanoma by activating host immunity
Authors: Chen Chia Hung , Weng Tzu-Han , Chuang Cheng-Hsun , Huang Kai-Yao , Huang Sih-Cheng , Chen Pin-Rong , Huang Hsiao-Hsuan , Huang Ling-Ya , Shen Pei-Chun , Chuang Po-Ya , Huang Hsiao-Yen , Wu Yi-Syuan , Weng Shun-Long , Liao Kuang-Wen
Published: March 2022

Background: Melanoma is a malignant cancer with high rates of recurrence, metastasis, and immunosurveillance evasion. Benefit for clinical treatments of melanoma, the transdermal neoadjuvant therapy could reduce surgery region and increase immunotherapy efficacy. Using cationic lipoplex (LPPC) encapsulated doxorubicin (DOX) and carrying CpG oligodeoxynucleotide; the nano-liposomal drug complex (LPPC-DOX-CpG) would have higher cytotoxicity and immunostimulatory activity than the originally unencapsulated DOX and CpG. In addition, CpG adsorption did not interfere with the release of drugs from the complexes. The liposomal-drug complexes were transdermally administered, allowing them to easily penetrate the skin within 12 h. After the application of the treatments, subcutaneous melanoma growth and systemic metastasis were monitored to assess therapeutic efficacy. Then, next-generation sequencing (NGS) data were used to explore the underlying mechanism. Results: Transdermal administration of LPPC-DOX-CpG dramatically suppressed subcutaneous melanoma growth. LPPC-DOX-CpG induced tumor cell apoptosis and recruited immune cells into the tumor area. Interestingly, animal studies also showed that the colonization and growth of spontaneously metastatic melanoma cells in the liver and lung were suppressed by transdermal treatment with LPPC-DOX-CpG. NGS analysis further revealed the activation of the exogenous apoptotic pathway as the major mechanism responsible for the therapeutic effect of LPPC-DOX-CpG. The chemo-attractant, IFN-g and NF-kB pathways were also triggered to recruit and activate the antigen presenting cells and effecter cells. Conclusions: Finally, we have successfully proved transdermal LPPC-DOX-CpG as a promising penetrative carrier to activate anti-tumor immunity against subcutaneous and metastatic tumor.
A smart insole system capable of identifying proper heel raise posture for chronic ankle instability rehabilitation
Authors: Kim Seung-Jong , Kim Jaewook , Kang Seonghyun
Published: March 2022

Heel raise is widely prescribed to patients with chronic ankle instability in order to strengthen the Peroneus Longus muscle (PL) which supports the weakened lateral collateral ligaments. While the exercise itself is intuitive, ankle orientation is of particular importance because heel raises performed with inversion do not well recruit the PL. This implies that proper execution is imperative and a means to assess heel raise training sessions is needed. In this study we present a smart insole system is capable of identifying heel raise events and its corresponding rise, hold and drop phases, which allows for a more descriptive analysis. The results from our heel raise sessions, which consist of four different variants performed by five healthy subjects, suggest that medial-lateral foot pressure distribution and foot orientation are needed to differentiate heel raises performed with ankle eversion and inversion. We go further and substantiate that proper execution, detected by our system, indeed leads to increased PL activation by analyzing the electromyography signals. We believe that the proposed system may provide clinicians with invaluable information regarding onsite as well as at-home training and possibly with biofeedback serve as foundation for software as a medical device.
The association between multiple meteorological factors and seasonal influenza A and B virus transmission in Macau
Authors: Ng HoiMan , Li Yusi , Zhang Teng , Lu Yiping , Wong ChioHang , Ni JinLiang , Zhao Qi
Published: March 2022

Background and objectives: Few studies evaluated the influence of meteorological factors on different influenza types activity in subtropical regions. The study aimed to explore the association between meteorological factors and the onset of influenza A (Flu-A) and B (Flu-B) in Macau.Methods: Daily influenza cases data were collected from Kiang Wu Hospital from January 1st, 2014 to December 31st, 2018 in Macau. Daily meteorological data were obtained from Macau Meteorological Service. The distributed lag non-linear model (DLNM) was used to estimate the effects of meteorological factors on the seasonal influenza outbreak.Results：Mean temperature had the peaking risks of both types at 4.0°C and 28.0°C. For the diurnal temperature range (DTR), the peaks of the cumulative relative risk (RR) of Flu-A were at 1.0°C and 5.0°C, while the cumulative RR of Flu-B increased as the DTR decreased. The association between influenza and relative humidity were showed a U-shape curve. The risk of influenza increased when the relative humidity was below 50% or above 90%. Both types increased significantly when the sunshine duration was below about 3.5 hours. Taking the median value as the reference, a significant cold effect was observed along 16-24 lag days in Flu-A. The lag effects were found in low-DTR, humid and short sunshine conditions in both types.Conclusions：This study revealed the complex non-linear association between meteorological factors and the different influenza types activity in Macau. The finding helped to develop an early warning system to improve the response capacity to influenza epidemics.
Dual Age-dependent function of KLF4 in Wnt regulation linked with TERT and Ctnnb1 in two differentiated and undifferentiated populations of SSCs
Authors: Sojoudi Kiana , Azizi Hossein , Skutella Thomas
Published: March 2022

KLF4 is a transcription factor involved in proliferation, differentiation, apoptosis and somatic cell reprogramming; thus, it has a critical role in accurate spermatogenesis. Currently, how KLF4 regulates the Wnt cascade has been discussed and suggested that this connection be related to TERT and Ctnnb1, but its regulatory mechanisms are still very vague. Contrary to the data of many researchers who have identified KLF4 as a factor in maintaining stemness, we have stated that KLF4 is a differentiating factor that its expression increases during spermatogenesis. Furthermore, we show how the regulatory loops of KLF4, TERT and Ctnnb1 through the Wnt pathway is age-dependent. Data collected during immunohistochemistry, immunocytochemistry and Fluidigm real-time RT-PCR besides in silico analyzes indicate that the performance of KLF4 is context-dependent.
A Novel Automated Planning Approach for Multi-Anatomical Sites Cancer in Raystation Treatment Planning System
Authors: Lou ZhaoYang , Mao RongHu , Li DingJie , Tian LingLing , Li Bing , Lei HongChang , Ge Hong
Published: March 2022

Background
Previous automated planning approach studies for Raystation were limited to a specific anatomical site cancer or a single treatment technique. The purpose of this study is to develop an automated planning approach in Raystation and evaluate its feasibility and performance in multiple clinical application scenarios.
Methods
Sixty patients, including 20 nasopharyngeal carcinoma (NPC), 20 esophageal carcinoma (ESCA) and 20 rectal cancer (RECA), who were treated with volumetric-modulated arc therapy for NPC and RECA and intensity-modulated radiotherapy for ESCA, were retrospectively enrolled in this study. An automated planning approach (Ruiplan), consisting of five key modules: prescription and OAR identification module, auxiliary structure generation module, dose prediction and objectives setting module, beam configuration module, plan fine-turning module, was developed by using the scripting platform of Raystation. Radiotherapy plans were re-generated both automatically by using Ruiplan and manually by experienced physicists. Target coverage, organs at risk (OARs) sparing, and planning efficiency of the automated plans (APs) and manual plans (MPs) were statistically compared using paired student t-test.
Results
For target coverage, APs yielded superior dose homogeneity in NPC and RECA, compared to MPs, while maintaining similar dose conformity for all studied anatomical sites. For OARs sparing, APs led to significant improvement in most OARs sparing, compared to MPs. The max dose of the lens, eyes and optic nerves in NPC, the V20 and mean dose of lungs, the V30, V40 and mean dose of heart in ESCA, the V30, V50 of bowel and V30 of femoral heads in CRCA were significantly decreased on average in APs compared to MPs (P < 0.05). The average planning time required for APs was reduced by more than 43% compared with MPs. Despite the increased monitor units (MUs) for NPC and RECA in APs, the beam-on time of APs and MPs had no statistical difference. Both the MUs and beam-on time of APs were significantly lower than that of MPs in ESCA (P < 0.05).
Conclusions
The developed Ruiplan was capable of generating high-quality treatment plans that were comparable to the MPs created by experienced physicists, in a variety of treatment techniques and cancer sites studied.
Single-cell sequencing reveals the landscape of the tumor microenvironment in malignant fibrous histiocytoma
Authors: Xia Yuan-Zheng , Yuan Liu-Liu , Chen Zhong , Qin Jian , Qin Cheng-Jiao , Bian Jing , Dong Rui-Fang , Yuan Tang-Bo , Xu Yi-Ting , Kong Ling-Yi
Published: March 2022

Background: Malignant fibrous histiocytoma (MFH) is an invasive pleomorphic soft tissue sarcoma with a high degree of malignancy and poor prognoses, and is prone to recurrence and metastasis. The pathophysiology remains elusive and its therapeutic options are limited. The 5-year recurrence rate of patients is 36% to 61%. Progress in single-cell RNA sequencing (scRNA-seq) provides an opportunity to dissect the pathophysiology of human diseases at unprecedented resolution, particularly in the diseases lacking animal models, such as MFH.Methods: We performed scRNA-seq on tumor tissues and adjacent muscle tissues from a patient with MFH. We identified Cell types and the corresponding marker genes by single-cell RNA sequencing. Malignant cells of fibroblasts were evaluated by CopyKAT analysis and differentially expressed genes of sequencing.Results: We identified PDCD1, CTLA4 and TIGIT as potential targets. We further showed that C2_Exhausted CD4+ Treg and C1_Exhausted CD8+ T cell highly expressed PDCD1, CTLA4 and TIGIT. Intervention via PD-1 immune checkpoint inhibitor (tirelizumab) enabled disease control and reduced tumor immunosuppression. Conclusion: Thus, scRNA-seq analyses guide a successful therapeutic intervention in the MFH patient, improve our understanding of complex human diseases and provide an alternative approach to personalized medicine.
NTN4 as a prognostic marker and associated with immune infiltrates in breast cancer
Authors: Yi Lili , Lei Yongqiang , Yuan Fengjiao , Tian Conghui , Chai Jian , Gu Mingliang
Published: March 2022

Netrin-4 (NTN4), a member of neurite guidance factor family, can promote neurite growth and elongation. NTN4 participates in breast cancer, however, whether NTN4 correlates with prognosis and immune infiltration in breast cancer remain unclear. This study aims to investigate prognostic landscape of NTN4 in breast cancer and relationship of NTN4 expression with immune infiltration. Breast carcinoma tissue were used to IHC. Expression pattern and prognostic value of NTN4 were explored in breast cancer using multiple databases, including UALCAN, TIMER, Kaplan-Meier Plotter and Prognoscan. Using TIMER database, relationships of NTN4 expression with tumor immune invasion and immune cell surface markers were evaluated. Transcription and survival of NTN4 were investigated in breast cancer based on cBioPortal database. STRING database was explored to identify molecular functions and signaling pathways downstream of NTN4. NTN4 expression was significantly lower in invasive breast carcinoma compared with adjacent non-malignant tissues. Promoter methylation of NTN4 in breast cancer exhibited different patterns. Low NTN4 expression was associated with poorer survival. NTN4 expression was significantly positively related to infiltration of CD8+ T cells, macrophages and neutrophils, whereas significantly negatively related to B cells and tumor purity. Association patterns were different from different subtypes. Different patterns of association between NTN4 expression and immune cell surface markers were revealed. Different subtypes of breast cancer carried different gene alterations. NTN4 was involved in mediating multiple biological processes including morphogenesis and migration.
The effects of ethyl lauroyl arginine hydrochloride( ELAH) in nasal spray on SARS-C0V-2
Authors: Gaffar Abdul , Thacore HR , Yun Seiyoung , Chemine Agnes , Ferrari Maria , Pal Ranagit , Wattay Lauren , Peterson Marnie L
Published: March 2022

In 2019, a coronavirus caused an outbreak of severe respiratory syndrome in Wuhan, China, and the virus was identified as severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The aim of the study was to investigate the broad-spectrum antiviral effects of ethyl lauroyl arginine hydrochloride (ELAH) against SARS-CoV-2 in vitro and in cell cultures. In vitro treatment of pseudovirus VSV-SARS-CoV-2 expressing the receptor binding domain of SARS-CoV-2 for 10 minutes at 37°C with 15 µg/ml and 110 µg/ml ELAH inhibited virus replication in Vero cells by 55% and over 90%, respectively. Intranasal administration of Syrian golden hamsters with SARS-CoV-2 pretreatedin vitro with ELAH for 10 minutes at 37°C not only inhibited the disease symptoms typically caused by SARS-CoV-2 in hamsters but also concomitantly reduced the total RNA viral load and the subgenomic RNA compared with the control non-ELAH group. In vitro treatment of the Delta variant of SARS-CoV-2 at 37°C for 10 minutes with 200 µg/ml ELAH inhibited replication in Vero E6 TMPRS-2 cells by 2 logs. To elucidate the mechanism of the action of ELAH, MRC-5 cells were pretreated with various concentrations of ELAH followed by infection with HCoV-229E. The data presented show that ELAH inhibited the cytopathic effects induced by the virus in MRC-5 cells. High-resolution electron microscopy of MRC-5 cells pretreated with ELAH prior to infection showed significantly reduced progeny virions on the surface of the infected cells. These results suggest that the primary mode of action of ELAH in MRC-5 cells is most likely due to the lack of attachment of the virus to the aminopeptidase N (APN) receptor, thus affecting the entry and subsequent replication of the virus in infected cells. The results presented in this study suggest that ELAH in nasal spray formula could be a promising virucidal agent for the prevention of coronavirus infections, including SARS-CoV-2 infections through the nasopharynx.
Assessment of level of compliance with the use of condoms among discordant HIV-infected couples in Kaduna State, Nigeria.
Authors: Danboyi Daniel Abba , Danjuma Lawal , Abdullahi Mas'ud , Sani Nura Muhamma
Published: March 2022

Background: The use of condoms is key in the global fight against HIV and AIDS. This helps to reduce the rate of infection and disease burden in the general population. Furthermore, it underscores the need to intermittently study or assess the level of compliance with the use of condoms as a preventive measure. Methods: The research was conducted to assess the level of compliance with condom use among HIV- infected discordant couples in Kaduna State Nigeria. The study was conducted in Antiretroviral (ART) treatment centers of 9 Local Government Areas of the 23 Local Government Areas in Kaduna State Nigeria. A total of 158 couples or 317 individuals were recruited for the study. A detailed questionnaire was used to collect data on demographics, the use of condoms, and the practice of unprotected sex.Results: The results obtained in the study showed that the majority of the respondents 224(70.7%) were unemployed. Most of the respondents 230(72.6%) never used a condom while 87(27.4%) did use condoms. The study also revealed that the Educational status of respondents had a strong association with their perceived attitude towards the use of condoms during sex (p-value 0.009) while attitude towards the practice of unprotected sex was statistically insignificant (p-value 0.404). There is a need to improve on the educational coverage for the entire population to mitigate the scourge of HIV transmission.
Bifurcated sleep in night shift workers alters circadian relationships in cardiovascular and temperature rhythms
Authors: Gupta Neelu Jain , Yadav Shivani , Malik Sweekriti
Published: March 2022

Shift workers are faced with sleep deficits and circadian disruption, a risk factor for hypertension. We recorded daily rhythms of body temperature and blood pressure (BP) of 28 healthy healthcare workers using thermometers and ambulatory BP monitors, respectively. Participants worked in in regular day (D) shift, regular night (N) shift or rotational shift (RS, i.e., RD/RN during day/night shift) work routines. Different shift regimes had variable effects on sleep quality and duration, thus affecting the waveform of daily BP/temperature rhythms. Therefore, this study aimed to assess circadian misalignment of BP/temperature rhythms under shift-work regimes. In D, 8-h night sleep and robust bimodal rhythmic patterns in SBP, DBP and temperature were observed. Among shift workers, N exhibited bifurcated sleep with long work-hour naps commensurate to the daily peak and dip in BP/temperature. The amplitude of the daily rhythm of BP and temperature was dampened in RS. Overall, cardiovascular rhythm disruption was greater in SBP than in DBP. RS exhibited higher temperatures throughout day-night shifts. Sleep bifurcation, although it immediately recompenses sleep deficits, may be associated with altered circadian waveforms of physiological rhythms, rendering long-term health consequences.
Potential of Professional Gowth of Foreign Language Teachers
Authors:   Education Development of Transbaikal Region, Elena V. Pasynkova
Published: March 2022

The article is concerned with the actual problem of the organization of continuing education of teachers in the Russian system of professional development of teachers, taking into account the requirements of regulatory documents: the Federal Law “On Education in the Russian Federation”, Federal State Educational Standards and the Professional Standard of a teacher. The author focuses on various forms of professional development of teachers of foreign languages, implemented by the State Educational Institution “Institute of Education Development of Transbaikal Region”, aimed at personal growth and self-realization of the teacher, as well as significant in the certification of the qualification category. Professional continuing education of teachers is considered by the author in specific types of activities, as well as components of the system of continuous professional development of teachers. The author of the article analyzes the leading trends and promising directions for the development of continuing education, the set of psychological and pedagogical competencies developed in the process of professional development and retraining, as well as in the course of innovation activities, the implementation of creative projects, and other inter-course activities. Special attention is paid to the need to organize language-oriented courses for the development of language competence of foreign language teachers. Updated information about informal models of professional development, new forms of events organized by the Institute of Education Development of Transbaikal Region are presented in the article.
ACCOUNTABILITY OF NORTH LAMPUNG PRIVATE ISLAMIC COLLEGE OF RELIGION
Authors:   Post-Graduate Program of Islamic Education Management Study Program, Raden Intan State Islamic University of Lampung, Indonesia., Sajdah Meilisa , Basyar Syaripudin ,   Post-Graduate Program of Islamic Education Management Study Program, Raden Intan State Islamic University of Lampung, Indonesia., Fakhri Jamal ,   Post-Graduate Program of Islamic Education Management Study Program, Raden Intan State Islamic University of Lampung, Indonesia., Dermawan Oki ,   Post-Graduate Program of Islamic Education Management Study Program, Raden Intan State Islamic University of Lampung, Indonesia.
Published: March 2022

PrivateIslamic Higher Education according to constitutionnumber4 of 2014are required to present information on the implementation of activities in an open, fast and precise manner to the public. For that t goal study this is for analyze about accountability, transparency, efficiency, effectiveness and participation Private Islamic Higher Education in North Lampung. Research method this is descriptive qualitative that collects primary and secondary data with method observation, interview and documentation. After that done data analysis with method reduction,display and verification of data so that could Becomes conclusion at the end analysis after inspection data validity. Research results this show that 1) Accountability of Private Islamic HigherEducationin North Lampung is running well and truly in accordance with the principles of good governance. 2) Transparency of universities so far in carrying out their duties has been getting better. 3) Implementation efficiency already walk with ok, staff academic understand will duties and responsibilities the answer supported with availability computers and facilitation other. Staff Academics are also responsible answer on service that doesnt appropriate, 4) Effectiveness of Private Islamic HigherEducationin North Lampung all staff, employees already know, understand the contents of the rules of behavior/ethical standards, punitive sanctions for violations of the rules of conduct/ethical standards. 5) Community participation with Universities is quite good in implementing higher education governance. Good Governance although it is still simple based on local values with objective conditions in Higher Education. increased accountability and community participation can be guaranteed in higher education.
THE ROLE OF EDUCATION AND SCIENTIFIC RESEARCH IN DEVELOPMENT IN PALESTINE
Authors:   The Role Of Education And Scientific Research In Development in Palestine., Gawdat Mahmoud Qabaha Mahmoud , Mahmoud Othman Othman ,   The Role Of Education And Scientific Research In Development in Palestine.
Published: March 2022

One of the most important duties of education is to enable individuals to develop and advance their societies through scientific research, especially the great acceleration in the era of knowledge, information and technology and its outputs. Therefore, this study sought to identify the role of education and scientific research in development in Palestine. During the study, the study concluded several results, most notably That education and scientific research in Palestine still suffers from some scientific, financial, administrative and social obstacles, and it is in dire need of development in all its branches, and that education and scientific research have an effective role in achieving development goals of all kinds in general and Palestine in particular, and that the amount of spending on it in Palestine is not It is still far below the level required globally, and in light of this result, the study made several recommendations, the most prominent of which were: the need to work to raise awareness of the importance of education and scientific research and apply its results in achieving comprehensive development in society, in addition to developing a strategy for the State of Palestine with regard to education and scientific research after Determining research priorities, leading to linking research with comprehensive development, working to provide the necessary assistance by universities and research centers to researchers, developing mechanisms for marketing the results of scientific research, publicizing its results and outputs, and strengthening coordination between research centers, institutions and government agencies.
COMMUNITY BASED REHABILITATION: PROVIDE BARRIER FREEENVIRONMENT AND DEVELOPMENTAL OPPORTUNITIES FOR DISABLED PEOPLE
Authors:   Prof. Rajendra Singh (Rajju Bhaiya) University, Prayagraj., Gautam Kavita
Published: March 2022

Community-based rehabilitation approach providesrehabilitation reduce poverty, equal opportunities, and social insertion for differently abled people at community level. Objective of CBR is to meet the needs of people with disabilitieswith the respect to health, educat ion, employment, social and economic empowerment issues (WHO 2010). Statement of the problems: I am going to find out the barriers which affect the available facilities for disabled people. Society treats disabled people in a different way as pity, feelings of sympathy, isolated or rejected. People often maintain social distance and consider disabled as outsiders. Many disable people feel discriminated at various levels in the family as well as in the community. Sometimes their families strongly supported them and even sometimes they might be over-protected by the family members. At the same time they might be rejected and left out from normal community life because of society humiliation and discrimination. In that conditions disabled people have very restricted and limited opportunities and choices for their own development. Objectives: The main focus of the study to find out how community based rehabilitation programme strategies will be effective to remove barriers in all stages and createmultiple opportunities and choices for the betterment of life of disabled people. In this backdrop the paper aims to find out how CBR helpful to provide and strengthentheir mental and physical abilities so they can understand themselves as normal human being in the society. Methodology: The research paper envisioned to find out the exercise of CBR service facility to PWDs, regulate whether differently abled have access to community based rehabilitation facilities, and recognize the CBR implementation challenges from the opinion ofcaregiversand parents, service providers. This whole paper is based on theoretical and literary approach. Data is collected from secondary sources such as books, research papers, journals, and reports etc.
THE VALIDITY OF VERBAL AGREEMENT ON THE PLANTATION PROFIT SHARING AGREEMENT IN THE VILLAGE OF NALAHIA, CENTRAL MALUKU
Authors: Berlianty Teng
Published: March 2022

This study aims to examine and analyze the legal position of the plantation profit sharing agreement in the form of an oral agreement and the validity of the oral agreement on the plantation production sharing agreement in the village of Nalahia, Central Maluku. This research is a sociolegal research, which is a combination research method between doctrinal law research methods and empirical legal research methods. This research was conducted in the village of Nalahia, NusalautSubdistrict, Central Maluku.This type of research data is primary data and secondary data obtained through literature study and interview techniques with respondents. Based on the results of the study, the plantation production sharing agreement in the village of Nalahia, NusalautSubdistrict, Central Maluku Regency met the legal requirements of the agreement as stated in Article 1320 of the Civil Code and was carried out in good faith. Therefore, this agreement is still considered valid and binding on both parties, namely the plantation owner and the sharecropper.
Identifikasi Keterampilan Gerak Manipulatif Anak Usia 6-7 Tahun Selama Masa Pandemi Covid- 19
Authors: Oktaria Anggita Dwi , Andika Windi Dwi
Published: March 2022

Penelitian ini bertujuan untuk mendeskripsikan keterampilan gerak manipulatif anak usia (6-7) tahun selama masa pandemi Covid-19 di Jalan Persada Ogan Ilir. Penelitian ini menggunakan jenis penelitian deskriptif kualitatif dengan empat subjek penelitian. Untuk teknik pengumpulan data peneliti menggunakan observasi, wawancara, dan dokumentasi. Analisis data yang digunakan adalah analisis data kualitatif Miles dan Huberman, meliputi pengumpulan data dari wawancara, observasi, dan dokumentasi, selanjutnya mereduksi data yang telah didapatkan dari lapangan, penyajian data dalam bentuk narasi singkat dan penarikan kesimpulan. Penelitian menggunakan empat subjek penelitian yaitu orang tua yang memiliki anak usia (6-7) tahun di Jalan Persada Ogan Ilir. Hasil penelitian menunjukkan bahwa keterampilan gerak manipulatif anak selama masa pandemi ini cukup baik dengan adanya kegiatan yang dapat merangsang gerak manipulatif anak seperti melempar, menangkap, memantulkan, menendang, menggelindingkan, dan mendorong.
Penerapan Pembelajaran Inklusi Pada Anak Usia Dini; Sebuah Solusi Layanan Pendidikan Khusus
Authors: Filasofa Lilif Muallifatul Khorida
Published: March 2022

Pembelajaran inklusi merupakan sistem layanan pendidikan yang terbuka dengan mengakomodasi semua peserta didik yang membutuhkan pendidikan layanan khusus tanpa diskriminatif dengan cara belajar bersama dalam suatu iklim dan proses pembelajaran sesuai dengan potensi, kemampuan, kondisi tanpa membeda-bedakan latar belakang kondisi sosial, ekonomi, suku bahasa serta perbedaan kondisi fisik maupun mental. Metode Penelitian adalah kualitatif dengan pendekatan fenomenologi, menggunakan analisis dokumen, observasi, wawancara, instrumen, dan dokumentasi.Hasil Penelitian Perencanaan pembelajaran yang dilakukan PAUD Inklusi di salah satu sekolah Kota Semarang adalah dengan membuat atau menyusun rencana pembelajaran harian yang disebut dengan lesson Plan, berupa Prota, Promes, RPPM, RPPH dan bulanan di setiap kelasnya.Pelaksanaan pembelajaran dilaksanakan secara halfday dengan diberikan stimulasi pendidikan oleh pendidik secara reguler. Pembelajaran menggunakan model klasikal dan kelompok, pada kegiatan pembukaan yaitu dengan circle time, kegiatan inti model privat individu, dilakukan di luar kelas di pendopo dan halaman. Faktor pendukung adanya sinergi kesinambungan kerjasama antara orang tua dengan pihak sekolah dalam menyukseskan pembelajaran, pendidik mendapatkan training dalam upaya memberikan layanan kepada anak dengan jenis hambatan yang berbeda-beda antara anak regular dan non regular.Faktor kendala adalah ketika orang tua tidak melaksanakan aturan-aturan yang telah disepakati bersama dan tidak menjalankan layanan ketika di rumah.
Complex network modeling of a river basin: an application to the Guadalquivir River in Southern Spain
Authors: Rodríguez-Alarcón R. , Lozano S.
Published: March 2022

In this paper, complex network analysis is used to analyze the structure of a whole river basin. A classification of nodes and edges that allow the river basin to be represented as a directed network is given. Both global and local characterization metrics are presented. Thus, centralization indexes, average path length, diameter, network efficiency, edge length, degree and strength distributions can be computed and interpreted. The closeness and betweenness centrality as well as the PageRank index of the different nodes can also be calculated and provide information on the relative position of each node in the network in terms of their average distance to upstream and downstream nodes, their reachability from other nodes, etc. In addition, a new centrality index based on the environmental impact of a potential spill or contaminant release on each node of the network has been applied. This type of centrality index is particularly useful in hydrologic networks and helps to identify key nodes from a contamination propagation point of view. The application of the proposed approach to the Guadalquivir River basin, in Southern Spain, is presented. Apart from validating the environmental impact centrality index, the most important finding is the scale-free character of the network and its consequences.
Scour model for circular compound bridge pier
Authors: Nimbalkar Padmakar , Rathod Praveen , Manekar Vivek , Bhalerao Anand
Published: March 2022

Scour is a complex phenomenon, whose complexity increases with the change in the geometry of the obstruction. Most investigations have been carried out on the scour process at a uniform pier. However, in reality, many bridge piers behave as non-uniform depending on the exposure of their foundation into the flow field. All the experimental investigations were carried out in the present study to understand the effect of pier geometry, the position of footing top with respect to bed level, and sediment mixtures (uniform and non-uniform) on local scour under clear water conditions. A total of 106 experiments were conducted in the present study with a different combination of pier models, sediment mixture, and footing top with respect to bed. A maximum scour depth model was developed using 182 data points consisting of experimental data (106) and extracted from the literature (76). To develop a model, a state-of-the-art artificial intelligence (AI) based modeling technique known as gene expression programming (GEP) was employed in this study. The GEP model was developed by using 130 data points and independent 52 data points for the model validation. The performance of the proposed scour model for the compound bridge pier was found to be satisfactory.
A review on the application of the DRASTIC method in the assessment of groundwater vulnerability
Authors: Patel Priyank , Mehta Darshan , Sharma Neeraj
Published: March 2022

Industrial and municipal wastes, agricultural contamination owing to pesticides and chemical hazards, seawater intrusion in coastal areas, and other factors damage groundwater. In several towns and industrial clusters across India, this is becoming a rising subject of concern. Groundwater is difficult to contaminate, but once contaminated, it is difficult to clean up. It is critical to attain this goal using a variety of aquifer vulnerability assessment approaches. All of these strategies rely on process models as well as statistical or overlay index methodologies. Groundwater vulnerability is a major topic of discussion due to declining groundwater levels and rising contamination, posing a serious threat to the environment and water sources. To identify the risk and to assess the vulnerability, extensive research has been carried out among all the methods based on different parameters and different indexes. The DRASTIC method is one of the most important and accurate of the overlay and index methods for the assessment of groundwater vulnerability. This research study is a systematic analysis of the available research articles on the applications of the DRASTIC and Modified DRASTIC (DRASTIC-L) performance management process on Geographical Information Systems (GIS). This research also reveals research gaps in the various groundwater vulnerability assessment approaches, as well as their limits and hypotheses. This study discovered that integrating GIS with DRASTIC is the most effective and accurate way for determining groundwater vulnerability. In agricultural, arid, semi-arid, and basaltic zones, the modified DRASTIC model also outperforms the traditional DRASTIC model.
EFFECT OF MODELED MICROGRAVITY ON THE LEVEL OF mRNA GENES ENCODING THE CYTOSKELETAL PROTEINS AND HISTONE ACETYLATION IN DROSOPHILA MELANOGASTER OVARIES
Authors:   Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Golubkova М.А. , Ogneva I.V. ,   Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow
Published: March 2022

Maintenance of the human reproductive health is vital in context of preserving living species in remote space missions. Changes in the gene expression due to microgravity can lead to degradation of fertility; however, the mechanism that triggers these changes is not fully understood. In our investigation we determined mRNA levels in the prime cytoskeletal proteins and analyzed histone methylation and acetylation activities in Drosophila melanogaster ovaries during 79-hr microgravity modeled with the use of a random positioning machine (RPM), and per os introduction of essential phospholipids. The results showed that the fruit flies exposure to modeled microgravity over the entire oogenesis cycle increased levels of mRNA genes encoding the cytoskeletal proteins in oocytes and that this can be, probably, associated with increased acetylation of the histone H3 Lys9 and Lys27. Essential phospholipids seem to prevent growth and/or initiate mRNA reductions in the majority of cytoskeleton elements in the flies exposed to modeled microgravity; however, the tubulin and lamin B binding proteins increased in the respective controls. Essential phospholipids leveled histone 3 Lys9 acetylation in modeled microgravity but enhanced Lys27 acetylation in the control.
La ciudad en expansión. Desigualdades urbanas en el acceso al agua en la Tercera Chica, San Luis Potosí, México
Authors: Gasca Moreno Claudia Teresa , Ávila Quijas Aquiles Omar
Published: March 2022

Este artículo presenta un acercamiento a la colonia Tercera Chica, ubicada en la ciudad de San Luis Potosí, en el estado del mismo nombre, en México. El objetivo es analizar las des-igualdades en el acceso al agua como resultado de la expansión de la ciudad. En este sentido, discutimos cómo estas se crean inherentes al conjunto de conceptos asociados a lo urbano y mostramos la adecuación de prácticas culturales en una comunidad periurbana que permiten hacerse del recurso a través de la implementación de estrategias diversas.
Un acercamiento al soneto contemporáneo en México
Authors: Pardo Ignacio Ballester
Published: March 2022

Este artículo trata de mostrar la evolución de la famosa composición poética llamada soneto en el país con más hispanohablantes, México. Para ello, como hilo conductor, traza los acentos rítmicos que tienen su origen en Italia y que ofrecen numerosas vertientes en la poesía mexi-cana contemporánea. Se concluye que la hipótesis del cambio de acento sáfico (en la cuarta sílaba) al enfático (en la sexta sílaba) demuestra que el ritmo que recaía al principio del verso va atrasándose en castellano por conversión de acentos rítmicos y antirrítmicos en pos de un patrón octasilábico castellano, que deja ver el análisis cualitativo. Asimismo, la vigencia que sigue teniendo el soneto después de ocho siglos evidencia las posibilidades que plantea un país en el que las vanguardias y neovanguardias del siglo XX ampliaron los límites de los catorce versos rimados, tan frecuentes pero tan poco estudiados en los últimos años.