bluuebunny/crossref_metadata_2025 huggingface dataset:
How Does the Environment Affect Wheat Yield and Protein Content Response to Drought? A Meta-Analysis
Authors: Wan Chenxi , Dang Pengfei , Gao Licheng , Wang Jiale , Tao Jincai , Qin Xiaoliang , Feng Baili , Gao Jinfeng
Published: June 2022

Wheat (Triticum aestivum L.) is one of the most significant cereal crops grown in the semi-arid and temperate regions of the world, but few studies comprehensively explore how the environment affects wheat yield and protein content response to drought by means of meta-analysis. Therefore, we collected data about grain yield (GY), grain protein yield (GPY), grain protein content (GPC), and grain nitrogen content (GNC), and conducted a meta-analysis on 48 previously published data sets that originate from 15 countries. Our results showed that drought significantly decreased GY and GPY by 57.32 and 46.04%, but significantly increased GPC and GNC by 9.38 and 9.27%, respectively. The responses of wheat GY and GNC to drought were mainly related to the drought type, while the GPY was mainly related to the precipitation. The yield reduction due to continuous drought stress (CD, 83.60%) was significantly greater than that of terminal drought stress (TD, 26.43%). The relationship between the precipitation and GPY increased in accordance with linear functions, and this negative drought effect was completely eliminated when the precipitation was more than 513 mm. Sandy soils and high nitrogen application level significantly mitigated the negative effects of drought, but was not the main factor affecting the drought response of wheat. Compared with spring wheat, the drought resistance effect of winter wheat was more obvious. Evaluation of these models can improve our quantitative understanding of drought on wheat yield and food security, minimizing the negative impact of drought on crop production.
Resistance to Powdery Mildew in Qingke Involves the Accumulation of Aromatic Phenolamides Through Jasmonate-Mediated Activation of Defense-Related Genes
Authors: Xu Congping , Zhan Chuansong , Huang Sishu , Xu Qijun , Tang Tang , Wang Yulin , Luo Jie , Zeng Xingquan
Published: June 2022

Powdery mildew (PM) leads to severe yield reduction in qingke (Hordeum vulgare L. var. nudum). Although studies have focused on identifying PM-related resistance genes, mechanistic insights into the metabolic regulation networks of resistance against PM have rarely been explored in qingke. Here, we integrated transcriptomic, proteomic and metabolomic data using PM-susceptible (G72) and PM-resistant (K69) accessions to systemically explore the mechanisms of PM resistance. The integrated results show that a rapidly transduction of jasmonic acid (JA) and (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile), and importantly, a inducing accumulation of aromatic PAs conferred qingke-specific resistance for PM stress. Functional analysis revealed that the four BAHD N-acyltransferase genes were responsible for the synthesis of aliphatic and aromatic PAs. The expression of the four genes are induced by methyl jasmonate (MeJA) and PM treatment. Co-expression network analysis shows that a histone lysine demethylase, JMJ705 gene, also induced by MeJA and PM treatment, had highly correlation with PAs biosynthesis. Chromatin immunoprecipitation (ChIP)-seq assays revealed that the level of trimethylated histone H3 lysine 27 (H3K27me3) of the four genes in MeJA and PM-treated plants was significantly reduced. Overall, our results suggest that a novel strategy for jasmonic acid signal-mediated demethylation controlling the accumulation of aromatic PAs to enhance plant immune resistance through removal of H3K27me3 and activating defense-related gene expression.
Differential Responses of Wheat (Triticum aestivum L.) and Cotton (Gossypium hirsutum L.) to Nitrogen Deficiency in the Root Morpho-Physiological Characteristics and Potential MicroRNA-Mediated Mechanisms
Authors: Xue Huiyun , Liu Jia , Oo Sando , Patterson Caitlin , Liu Wanying , Li Qian , Wang Guo , Li Lijie , Zhang Zhiyong , Pan Xiaoping , Zhang Baohong
Published: June 2022

Understanding the mechanism of crop response to nitrogen (N) deficiency is very important for developing sustainable agriculture. In addition, it is unclear if the microRNA-mediated mechanism related to root growth complies with a common mechanism in monocots and dicots under N deficiency. Therefore, the root morpho-physiological characteristics and microRNA-mediated mechanisms were studied under N deficiency in wheat (Triticum aestivum L.) and cotton (Gossypium hirsutum L.). For both crops, shoot dry weight, plant dry weight and total leaf area as well as some physiological traits, i.e., the oxygen consuming rate in leaf and root, the performance index based on light energy absorption were significantly decreased after 8 days of N deficiency. Although N deficiency did not significantly impact the root biomass, an obvious change on the root morphological traits was observed in both wheat and cotton. After 8 days of treatment with N deficiency, the total root length, root surface area, root volume of both crops showed an opposite trend with significantly decreasing in wheat but significantly increasing in cotton, while the lateral root density was significantly increased in wheat but significantly decreased in cotton. At the same time, the seminal root length in wheat and the primary root length in cotton were increased after 8 days of N deficiency treatment. Additionally, the two crops had different root regulatory mechanisms of microRNAs (miRNAs) to N deficiency. In wheat, the expressions of miR167, miR319, miR390, miR827, miR847, and miR165/166 were induced by N treatment; these miRNAs inhibited the total root growth but promoted the seminal roots growth and lateral root formation to tolerate N deficiency. In cotton, the expressions of miR156, miR167, miR171, miR172, miR390, miR396 were induced and the expressions of miR162 and miR393 were inhibited; which contributed to increasing in the total root length and primary root growth and to decreasing in the lateral root formation to adapt the N deficiency. In conclusion, N deficiency significantly affected the morpho-physiological characteristics of roots that were regulated by miRNAs, but the miRNA-mediated mechanisms were different in wheat and cotton.
Genomic Prediction Strategies for Dry-Down-Related Traits in Maize
Authors: Ni Pengzun , Anche Mahlet Teka , Ruan Yanye , Dang Dongdong , Morales Nicolas , Li Lingyue , Liu Meiling , Wang Shu , Robbins Kelly R.
Published: June 2022

For efficient mechanical harvesting, low grain moisture content at harvest time is essential. Dry-down rate (DR), which refers to the reduction in grain moisture content after the plants enter physiological maturity, is one of the main factors affecting the amount of moisture in the kernels. Dry-down rate is estimated using kernel moisture content at physiological maturity and at harvest time; however, measuring kernel water content at physiological maturity, which is sometimes referred as kernel water content at black layer formation (BWC), is time-consuming and resource-demanding. Therefore, inferring BWC from other correlated and easier to measure traits could improve the efficiency of breeding efforts for dry-down-related traits. In this study, multi-trait genomic prediction models were used to estimate genetic correlations between BWC and water content at harvest time (HWC) and flowering time (FT). The results show there is moderate-to-high genetic correlation between the traits (0.24–0.66), which supports the use of multi-trait genomic prediction models. To investigate genomic prediction strategies, several cross-validation scenarios representing possible implementations of genomic prediction were evaluated. The results indicate that, in most scenarios, the use of multi-trait genomic prediction models substantially increases prediction accuracy. Furthermore, the inclusion of historical records for correlated traits can improve prediction accuracy, even when the target trait is not measured on all the plots in the training set.
Fine Mapping of Two Major Quantitative Trait Loci for Rice Chalkiness With High Temperature-Enhanced Additive Effects
Authors: Yang Weifeng , Hao Qingwen , Liang Jiayan , Tan Quanya , Luan Xin , Lin Shaojun , Zhu Haitao , Bu Suhong , Liu Zupei , Liu Guifu , Wang Shaokui , Zhang Guiquan
Published: June 2022

Chalkiness is a crucial determinant of rice quality. During seed filling period, high temperature usually increases grain chalkiness, resulting in poor grain quality. Rice chalkiness was controlled by quantitative trait loci (QTLs) and influenced by environmental conditions. In this study, we identified two single-segment substitution lines (SSSLs) 22–05 and 15–06 with significantly lower percentage of grain chalkiness (PGC) than recipient Huajingxian 74 (HJX74) over 6 cropping seasons. Two major QTLs for chalkiness, qPGC5 and qPGC6, were located by substitution mapping of SSSLs 22–05 and 15–06, respectively. qPGC5 was located in the 876.5 kb interval of chromosome 5 and qPGC6 was located in the 269.1 kb interval of chromosome 6. Interestingly, the PGC of HJX74 was significantly different between the two cropping seasons per year, with 25.8% in the first cropping season (FCS) and 16.6% in the second cropping season (SCS), while the PGC of SSSLs 22–05 and 15–06 did not significantly differ between FCS and SCS. The additive effects of qPGC5 and qPGC6 on chalkiness in the SSSLs were significantly greater in FCS than in SCS. These results showed that qPGC5 and qPGC6 had major effects on chalkiness and the SSSL alleles were more effective in reducing chalkiness under high temperature condition in FCS. The fine-mapping of the two QTLs will facilitate the cloning of genes for chalkiness and provide new genetic resources to develop new cultivars with low chalkiness even under high temperature condition.
Different Levels of Context-Specificity of Teacher Self-Efficacy and Their Relations With Teaching Quality
Authors: Thommen Désirée , Grob Urs , Lauermann Fani , Klassen Robert M. , Praetorius Anna-Katharina
Published: June 2022

On the basis of Bandura’s social cognitive theory, researchers often assume that a teachers’ self-efficacy (TSE) will have a positive effect on teaching quality. However, the available empirical evidence is mixed. Building on previous research into TSE, we examined whether assessing class-/task-specific TSE gives a more accurate indication of the associations between TSE assessments and student-rated teaching quality. The analyses were based on the English sample of the Teaching and Learning International Survey (TALIS) Video Study. Mathematics teachers (N= 86) rated their self-efficacy beliefs using generalized task-specific TSE items and class-/task-specific TSE items. Their students (N= 1,930) rated the quality of teaching in their math class. Multilevel regression analyses revealed stronger associations between student-rated teaching quality and class-/task-specific TSE than generalized task-specific TSE. We discuss possible reasons for these results and outline the potential benefits of using class-specific assessments for future TSE research.
Impact of Immediacy of Feedback on Continuous Intentions to Use Online Learning From the Student Perspective
Authors: Yu Rong , Cai Xuerui
Published: June 2022

The immediacy of feedback in media is emerging to enhance the interactivity of online experience for users. There is a gap in the study to explore the impacts of the immediacy of feedback on continuous intentions to use online learning from the student perspective. This study aims to fill the gap to investigate the impacts of the immediacy of feedback on students’ continuous intentions to use online learning. This study utilizes the technology acceptance model (TAM) and expectation theory model (ETM) to conceptualize the effect of the immediacy of feedback on student continuous intentions to use online learning in terms of the mediation effect of Perceived Ease of Use (PEOU), Perceived Usefulness (PU), satisfaction, and attitude of students for continuous intentions to use online learning. An online survey of higher education students with experience in online learning is conducted to test the proposed hypothesis. The collected data are analyzed by using structural equation modeling (SEM) to establish the proposed hypothesis. The findings reveal that the immediacy of feedback from the media has a strong association with PEOU, PU, students’ attitudes, students’ satisfaction, and ultimately toward the continuous intentions to use online line learning in future. The study set key theoretical and practical insights to pave the way for future research.
A Corpus Study of Lexical Bundles Used Differently in Dissertations Abstracts Produced by Chinese and American PhD Students of Linguistics
Authors: Bao Kai , Liu Meihua
Published: June 2022

This study examined lexical bundles (LBs) used differently by Chinese and American PhD students of linguistics in their dissertation abstracts. Two corpora were built, with each having 700 dissertation abstracts produced by Chinese and American PhD students of linguistics, respectively. The study then used lexical analysis software to retrieve frequently used three-word LBs, from which LBs having different frequencies at a significant level across the two corpora were identified and termed as bundles used differently (BUDs). BUDs were then categorized and analyzed manually in terms of structure, function, and distribution in rhetorical moves. The major findings were: (1) 57.14% of the frequently used LBs were BUDs, of which 90.67% had occurrences in both corpora. The BUDs distributed inequivalently across categories and moves, with the text-oriented category and the move of Result having the most BUDs; and (2) BUDs exhibited two major patterns: the Chinese and American students filled different constituents into structurally and functionally similar constructions, and used LBs of dissimilar functions to fulfill the same communicative purposes. These findings indicate that variations in LB use have a high pedagogic value and confirm the need for using corpora to identify and teach core genre-specific vocabularies to second/foreign language learners.
Which Seat Facilitates the Detection of Off-Seat Behaviours? An Inattentional Blindness Test on Location Effect in the Classroom
Authors: Cao Shuqin , Wei Xiuying , Hu Jiangbo , Zhang Hui
Published: June 2022

Off-seat behaviour refers to students leaving their seats and walking out of a classroom without the teacher noticing. This behaviour occurs in special education for students with certain special needs, which would lead to serious safety problems. This study carried out an inattentional blindness test to explore whether the location of seats in classrooms would impact teachers’ detection rate regarding off-seat behaviours. The participants were 126 pre-service teachers (Mage= 18.72 ± 0.723; 92% female) who were invited to perform the primary task of counting students raising their hands up whilst the disappearance of one of the students was introduced as an unexpected occurrence. The results show that peripheral seats were more detectable than the central ones for the teachers to notice the “missing student.” Meanwhile, the left and below oriented seats were more likely to be ignored compared to those that were right and upper oriented. These results suggest the existence of a location effect in the classroom that is associated with teachers’ attention regarding off-seat behaviour. This study has implications for classroom management in terms of arranging students’ seats appropriately to assist in increasing teachers’ identification of this hazard.
The Impact of Psychological Health on Patient Recovery After Arthroplasty
Authors: Zhang Zhen , Xing Qiqi , Zhong Da , Pan Yixiao , He Tailai , Hu Yihe , Wang Long
Published: June 2022

Purpose

The purpose of this study was to determine the relationship between psychological health and postoperative recovery and satisfaction in patients undergoing total joint arthroplasty (TJA).

Methods

We prospectively enrolled patients undergoing TJA from July 2019 to December 2020. A psychological evaluation was conducted according to the Hospital Anxiety and Depression Scale (HADS). Based on the preoperative HADS scores, we grouped the patients into two groups: the symptomatic group and the asymptomatic group. Data on the Harris Hip Score (HHS), Knee Society Knee Scoring System (KSS), Forgotten Joint Score-12 (FJS-12), Short Form-12 (SF-12), and Numeric Rating Scale (NRS) for pain in these two groups were collected preoperatively and postoperatively. Then, these data were analyzed by Statistical Package for Social Sciences (SPSS) version 19.

Results

The final cohort consisted of 80 patients. Patients undergoing TJA had significantly decreased HADS and NRS scores and improved HHS, KSS, SF-12, and FJS-12 scores (all p < 0.001). Compared with the symptomatic group, the asymptomatic group showed better postoperative recovery (p < 0.05), especially after total knee arthroplasty (TKA) (p < 0.05). Good postoperative recovery positively impacted the patients’ postoperative psychological state.

Conclusion

Finally, the psychological state can affect recovery after TJA, and successful TJA can help improve patients’ psychological states, especially after TKA.
An Eye Tracking and Event-Related Potentials Study With Visual Stimuli for Adolescents Emotional Issues
Authors: Wang Quan , Wei Xiaojie , Dang Ruochen , Zhu Feiyu , Yin Shaokang , Hu Bingliang
Published: June 2022

Background

Psychological issues are common among adolescents, which have a significant impact on their growth and development. However, the underlying neural mechanisms of viewing visual stimuli in adolescents are poorly understood.

Materials and Methods

This study applied the Chinese version of the DSM-V self-assessment scales to evaluate 73 adolescents’ psychological characteristics for depressive and manic emotional issues. Combined with eye-tracking and event-related potential (ERP), we explored the characteristics of their visual attention and neural processing mechanisms while freely viewing positive, dysphoric, threatening and neutral visual stimuli.

Results

Compared to controls, adolescents with depressive emotional tendencies showed more concentrated looking behavior with fixation distribution index than the controls, while adolescents with manic emotional tendencies showed no such trait. ERP data revealed individuals with depressive tendencies showed lower arousal levels toward emotional stimuli in the early stage of cognitive processing (N1 amplitude decreased) and with prolonged reaction time (N1 latency increased) than the control group. We found no significant difference between the manic group and the control group. Furthermore, the depression severity scores of the individuals with depressive tendencies were negatively correlated with the total fixation time toward positive stimuli, were negatively correlated with the fixation distribution index toward threatening stimuli, and were positively correlated with the mean N1 amplitudes while viewing dysphoric stimuli. Also, for the individuals with depressive tendencies, there was a positive correlation between the mean N1 amplitudes and the fixation time on the area of interest (AOI) while viewing dysphoric stimuli. For the individuals with manic tendencies, the manic severity scores of the individuals with manic tendencies were positively correlated with the total fixation time toward the positive stimuli. However, no significant correlations were found between the manic severity scores and N1 amplitudes, and between N1 amplitudes and eye-tracking output variables.

Conclusion

This study proposes the application of eye-tracking and ERP to provide better biological evidence to alter the neural processing of emotional stimuli for adolescents with emotional issues.
Development Work in Swedish Eldercare: Resources for Trustworthy, Integrated Managerial Work During the COVID-19 Pandemic
Authors: Dellve Lotta , Williamsson Anna
Published: June 2022

The extensive needs for developments of eldercare addressing working conditions, care quality, influence, and safety was highlighted during the pandemic. This mixed-method study contribute with knowledge about capability-strengthening development work and its importance for trustworthy managerial work, before and during the COVID-19 pandemic. Questionnaire data and narratives from first-line managers immediately before (n = 284) and 16 months into the pandemic (n = 189), structured interviews with development leaders (n = 25), and documents were analyzed. The results identify different focuses of development work. Strategic-level development leaders focused the strengthening of old adults' capabilities. While operational-level leaders approached strengthening employees' capability. First-line managers' rating of their trustworthy managerial work decreased during the pandemic and was associated with their workload, development support and capability-strengthening projects focusing employees' resources. The study demonstrates the gap between strategic and the operational levels regarding understanding of capability set and needed resources for strengthening capabilities and trustworthy, integrated managerial work regarding safety, influence, and quality conditions for old adults and employees.
The Association Between Long Working Hours of Parents and Dyslipidemia in Their Children
Authors: Ahn Joonho , Lee Dong-Wook , Kang Mo-Yeol , Myong Jun-Pyo , Chung Mi Hae , Kim Hyoung-Ryoul , Lee Jongin
Published: June 2022

The purpose of our study is to examine the association between children's low-density lipoprotein cholesterol (LDL-C) levels and their parents' working hours. We used data from the 2010–2018 Korea National Health and Nutrition Examination Survey in which lipid profile samples of 3,799 children were eligible. Logistic regression analyses were used with an outcome variable of the dichotomous LDL-C group and an exposure variable of the father's and mother's working hours, respectively. In logistic regression models adjusted for age, sex, household income, education level of children and parents, and working hours of the parents, mothers' working hours more than 52 h per week were significantly associated with their children's dyslipidemia [OR 2.14, 95% confidence interval (CI) 1.33–3.47] compared to working 40 h or less, whereas fathers' working hours did not show statistical significance (OR 1.08, 95% CI 0.71–1.66) in the same manner. The association was greatest for elementary school students for mothers working more than 52 h per week (OR 3.42, 95% CI 1.64–7.14) compared to those working hours 40 h per week or less. Mothers' long working hours were associated with a higher prevalence of dyslipidemia in their children. The association was strongest for elementary school students. Proper working time of parents is required for their children's health.
Individual Factors Associated With COVID-19 Infection: A Machine Learning Study
Authors: Ramírez-del Real Tania , Martínez-García Mireya , Márquez Manlio F. , López-Trejo Laura , Gutiérrez-Esparza Guadalupe , Hernández-Lemus Enrique
Published: June 2022

The fast, exponential increase of COVID-19 infections and their catastrophic effects on patients' health have required the development of tools that support health systems in the quick and efficient diagnosis and prognosis of this disease. In this context, the present study aims to identify the potential factors associated with COVID-19 infections, applying machine learning techniques, particularly random forest, chi-squared, xgboost, and rpart for feature selection; ROSE and SMOTE were used as resampling methods due to the existence of class imbalance. Similarly, machine and deep learning algorithms such as support vector machines, C4.5, random forest, rpart, and deep neural networks were explored during the train/test phase to select the best prediction model. The dataset used in this study contains clinical data, anthropometric measurements, and other health parameters related to smoking habits, alcohol consumption, quality of sleep, physical activity, and health status during confinement due to the pandemic associated with COVID-19. The results showed that the XGBoost model got the best features associated with COVID-19 infection, and random forest approximated the best predictive model with a balanced accuracy of 90.41% using SMOTE as a resampling technique. The model with the best performance provides a tool to help prevent contracting SARS-CoV-2 since the variables with the highest risk factor are detected, and some of them are, to a certain extent controllable.
Comparison of the First and Second Wave of Infections by SARS-CoV-2: A Retrospective and Longitudinal Study From a Primary Health Care Center in Santiago of Chile
Authors: Acuña-Castillo Claudio , Inostroza-Molina Ailen , Castro Sergio A. , Molina-Cabrera Sonia , Leiva-Salcedo Elías , Riquelme Denise , Luraschi Roberto , Barrera-Avalos Carlos , Vallejos-Vidal Eva , Mella-Torres Andrea , Valdés Daniel , Torres Claudio , Maisey Kevin , Escobar Alejandro , Reyes-Cerpa Sebastián , Toro-Ascuy Daniela , Imarai Mónica , Reyes-López Felipe E. , Sandino Ana María
Published: June 2022

The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Many countries have reported the experience of at least two contagion waves, describing associated mortality rates and population behavior. The analysis of the effect of this pandemic in different localities can provide valuable information on the key factors to consider in the face of future massive infectious diseases. This work describes the first retrospective and comparative study about behavior during the first and second waves of the COVID-19 pandemic in Chile from a primary Healthcare Center. From 19,313 real-time quantitative PCR (RT-qPCR) tests assessed, the selected 1,694 positive diagnostics showed a decrease in mortality rate in the second wave (0.6%) compared with the first (4.6%). In addition, we observed that infections in the second wave were mainly in young patients with reduced comorbidities. The population with a complete vaccination schedule shows a decrease in the duration of symptoms related to the disease, and patients with more comorbidities tend to develop severe illness. This report provides evidence to partially understand the behavior and critical factors in the severity of the COVID-19 pandemic in the population of Santiago of Chile.
Epidemiological Characteristics of COVID-19 and Effective Public Health Interventions in Shenzhen, China
Authors: Li Guiyu , Lin Jiyong , Xu Danping
Published: June 2022

Objectives:

This study aims to analyze and summarize the epidemic characteristics of coronavirus disease 2019 (COVID-19), and the public heath interventions in Shenzhen from 1 January 2022 to 4 April 2022, hoping to provide useful reference for resurgence.

Methods

Data were extracted from the website of Shenzhen Municipal Health Commission from 1 January 2022 to 4 April 2022. The number of new indigenous patients, imported patients, symptomatic and asymptomatic patients, age, gender, regional distribution, screening routes, and clinical subtype were analyzed. The public health interventions were summarized and described.

Results

There have been 1,215 new indigenous cases and 1,447 imported cases in Shenzhen from 1 January 2022 to 4 April 2022. The age group of the indigenous cases range from 2 months to 92 years. The median age was 35.0. The male-to-female ratio was 1.13 (623:551). The number of symptomatic and asymptomatic patients were 930 (76.5%) and 285 (23.5%), respectively, without death. Shenzhen has experienced three outbreaks. Futian District has the large proportion of confirmed cases (55.8%), followed by Nanshan (13.5%), and Baoan District (13.5%). The indigenous confirmed cases were mainly screened from close contacts under quarantine observation (632 cases, 53.8%), key areas (304 cases, 25.9%), key crowds (93 cases, 7.9%), and communities (145, 12.4%). Among the imported cases outside the Chinses Mainland, China's Hong Kong had the largest number of confirmed cases (n = 1,368), followed by Singapore (n = 18), South Korea (n = 18), and Japan (n = 14). The Shenzhen government quickly implemented effective measures, including citywide screening, quarantine, tracking, classified management for different groups and the dividing epidemic-hit communities, villages into three regions (sealed area, controlled area, and prevention area), and expand the capacity of designated hospitals, etc., which effectively controlled the outbreaks. By 4 April 2022, no new local cases had been reported.

Conclusions

Three novel COVID-19 outbreaks occurred in Shenzhen between 1 January to 4 April 2022, linked to importation from outside the Chinese Mainland and subsequently caused the local transmission. The measures of citywide testing–tracking–classified management by risk level have effectively controlled the epidemic and should be continued to prevent resurgence.
Increasing Biodiversity and Land-Use Efficiency Through Pea (Pisum aestivum)-Canola (Brassica napus) Intercropping (Peaola)
Authors: Madsen Isaac J. , Parks Janice M. , Friesen Maren L. , Clark Robert E.
Published: June 2022

Intercropping is an ancient agricultural management practice quickly re-gaining interest in mechanized agricultural systems. Mechanized management practices have led to a decreased biodiversity at the macro- and micro-fauna levels. These agricultural practices have also resulted in the degradation of soil and long-term inefficiencies in land, water, and nutrients. The inland Pacific Northwest (iPNW) of the United States of America is a wheat-dominated cropping system. The integration of winter and spring legumes and oilseeds has improved the biodiversity and nutrient-use efficiency of the cropping systems. This article examines the feasibility of pea-canola (peaola) intercropping in dryland production systems of the iPNW. In two site years, small plot peaola trials were established near Davenport, WA. Overall, the land equivalence ratio (LER) of peaola was found to be 1.46, showing an increase in efficiency of the system. Increasing the N fertilizer application rates did not affect peaola yield, indicating that peaola has low demand for N inputs. The effects of peaola on insects and bacterial diversity were examined on replicated large scale strip trials. Peaola was found to have significantly greater numbers of beneficial insects than the monoculture controls. There were no significant differences between the diversity of the soil bacterial communities found in peaola vs. pea and canola monocultures. However, we found that the strict core soil bacterial microbiome of peaola was larger than the monocultures and included core members from both the canola and pea soil microbiomes. In conclusion, the widespread adoption of peaola would likely increase the biodiversity and increase the land use efficiency of dryland production systems in the iPNW.
Mitochondrial Gene Diversity and Host Specificity of Isospora in Passerine Birds
Authors: Kubiski Steven V. , Witte Carmel , Burchell Jennifer A. , Conradson Dylan , Zmuda Alexandra , Barbon Alberto Rodriguez , Vilches-Moure Jose G. , Felt Stephen A. , Rideout Bruce A.
Published: June 2022

Isosporainfections are common in both wild and captive passerine species. Many bird species have been shown to have co-evolved with a particular species ofIsospora. Disease can range from subclinical to severe and fatal, making infection and transmission of this parasite a concern for birds under managed care, particularly in institutions housing endangered species for breeding and reintroduction purposes. Whether birds in mixed-species enclosures represent a risk factor for severe isosporiasis due to infection with non-host-adapted strains is of concern for institutions managing these populations. To begin answering this question, we sought to characterize the host-specificity ofIsosporaspp. in a large number of passerine birdsviaretrospective sequencing of mitochondrial gene cytochromecoxidase subunit I (COI). Despite outliers,Isosporasequences largely grouped by host species and/or host family. Additional research is warranted into the degree of interspecies transmission and host-switching ofIsosporaparasites, and risk factors for the development of severe disease in passerine birds.
Genome-Wide Characterization of QYYZ-Like PRRSV During 2018–2021
Authors: Xu Hu , Xiang Lirun , Tang Yan-Dong , Li Chao , Zhao Jing , Gong Bangjun , Sun Qi , Leng Chaoliang , Peng Jinmei , Wang Qian , Zhou Guohui , An Tongqing , Cai Xuehui , Tian Zhi-Jun , Zhang Hongliang , Song Mingxin
Published: June 2022

In the last decade, the emergence of QYYZ-like porcine reproductive and respiratory syndrome virus (PRRSV) has attracted increasing attention due to the high incidence of PRRSV mutation and recombination. However, the endemic status and genomic characteristics of the QYYZ-like strains are unclear. From 2018 to October 2021, 24 QYYZ-like PRRSV isolates were obtained from 787 PRRSV-positive clinical samples. Only one QYYZ-like positive sample was from a northern province, and the rest were from central and southern provinces. We selected 9 samples for whole-genome sequencing, revealing genome lengths of 15,008–15,316 nt. We retrieved all the available whole-genome sequences of QYYZ-like PRRSVs isolated in China from 2010 to 2021 (n = 28) from GenBank and analyzed them together with the new whole-genome sequences (n = 9). Phylogenetic tree analysis based on the ORF5 gene showed that all QYYZ-like PRRSV strains belonged to sublineage 3.5 but were clustered into three lineages (sublineage 1.8, sublineage 3.5, and sublineage 8.7) based on whole-genome sequences. Genomic sequence alignment showed that QYYZ-like strains, have characteristic amino acids insertions or deletions in the Nsp2 region (same as NADC30, JXA1 and QYYZ) and that thirteen strains also had additional amino acid deletions, mostly between 468 and 518 aa. Moreover, QYYZ-like strains (sublineage 3.5) have seven identical characteristic amino acid mutations in ORF5. Recombination analysis revealed that almost all QYYZ-like complete genome sequences (36/37) were products of recombination and mainly provided structural protein fragments (GP2-N) for the recombinant strains. Overall, QYYZ-like strains were mainly prevalent in central and southern China from 2018 to 2021, and these strains provided recombinant fragments in the PRRSV epidemic in China.
Safety Analysis, Design and Evaluation of 2-DOF Parallel Lifting Actuator on Stereo Parking Robot
Authors: Jiang Jingang , Tan Qiyun , He Tianhua , Sun Jianpeng , Zhang Jiawei
Published: June 2022

Stereo garage technology can effectively alleviate the problem of parking difficulties, but the safety problems of its actuators, via which the core of stereo parking function can be realized, seriously affect its promotion and further development. In this paper, a two degrees of freedom (2-DOF) parallel lifting actuator for a stereo parking robot is designed by researching the type synthesis of the mechanism based on the screw theory. The limb constrained triangle method, the flexibility of limb constrained triangle, and the failure probability are proposed to determine the final configuration of the parallel lifting actuator. Then, this paper completes the dimensional optimization of the parallel lifting actuator based on the multi-motion performance indexes and kinematic analysis, which improves the safety and stability of the actuator. Finally, this paper verifies the validity of the parallel lifting actuator by establishing a parallel lifting actuator verification model system. By verifying the dynamic characteristics of the model mobile platform under different load conditions, it is proven that the kinematic stability of the mobile platform decreases with the increase of load mass under load conditions. Additionally, through practical application experiment, it is proven that the parallel lifting mechanism can effectively alleviate the parking difficulty problem.
Real-Time Temperature Distribution Monitoring in Chinese Solar Greenhouse Using Virtual LAN
Authors: Yang Shiye , Liu Xin , Liu Shengyan , Chen Xinyi , Cao Yanfei
Published: June 2022

The internal air temperature of Chinese solar greenhouse (CSG) has the problem of uneven spatial and temporal distribution. To determine temperature distribution at different locations, we designed a greenhouse temperature real-time monitoring system based on virtual local area network (VLAN) and estimate, including interpolation estimation module, data acquisition, and transmission module. The temperature data were obtained from 24 sensors, and the Ordinary Kriging algorithm estimated the temperature distribution of the whole plane according to the data. The results showed that the real-time temperature distribution monitoring method established was fast and robust. In addition, data validity rate for VLAN technology deployed for data transmission was 2.64% higher than that of cellular network technology. The following results are obtained by interpolation estimation of temperature data using gaussian model. The average relative error (ARE) of estimate, mean absolute error (MAE), root mean square error (RMSE), and determination coefficient (R2) were −0.12 °C, 0.42 °C, 0.56 °C, and 0.9964, respectively. After simple optimization of the number of sensors, the following conclusions are drawn. When the number of sensors were decreased to 12~16, MAE, RMSE, and R2 were 0.40~0.60 °C, 0.60~0.80 °C, and >0.99, respectively. Furthermore, temperature distribution in the greenhouse varied in the east–west and north–south directions and had strong regularity. The calculation speed of estimate interpolation algorithm was 50~150 ms, and greenhouse Temperature Distribution Real-time Monitoring System (TDRMS) realized simultaneous acquisition, processing, and fast estimate.
Critical P, K and S Concentrations in Soil and Shoot Samples for Optimal Tedera Productivity and Nodulation
Authors: Real Daniel , Bennett Richard G. , Nazeri Nazanin K. , Weaver David M.
Published: June 2022

Tedera is a forage legume that can provide out-of-season green feed in Mediterranean climates. To date, growers have had no comprehensive soil nutrition guidelines to optimise tedera production. We undertook field and glasshouse studies to understand tedera’s macronutrient requirements. Three field experiments were sown with tedera cv. Lanza® at Cunderdin, Dandaragan and Three Springs in Western Australia. These experiments evaluated seven levels of phosphorus (P) (0–30 kg ha−1) and potassium (K) (0–80 kg ha−1) and two combined treatments with P and K. Glasshouse pot experiments were conducted using tedera cultivars Lanza® and Palma and lucerne cultivar SARDI Grazer. Ten concentrations of added P (0–256 mg kg−1), ten of K (0–256 mg kg−1) and ten of sulphur (S) (0–16 mg kg−1) were tested. There was no significant response to P or K in field soils at Cunderdin or Three Springs. There was no response to K at Dandaragan, but P produced a positive response in the July and October growing season cuts. In the glasshouse, tedera cultivars reached peak productivity at lower soil Colwell P (7.6 to 12 mg kg−1) than lucerne (22 mg kg−1). Lanza® had a moderate biomass response, and Palma did not show a significant response to Colwell K (0.8 to 142 mg kg−1) or soil S (1.3 to 12.5 mg kg−1). Nodulation was greatly reduced at the extremes in P and K treatments. For the first time, these field and glasshouse results have allowed us to establish guidelines for optimal soil nutrition for tedera that growers can use to benchmark the soil or shoot nutrient status of their tedera pastures and assess the economic benefit of correcting deficiencies.
Morphoagronomic Evaluation of Yellow Pitahaya (Selenicereus megalanthus Haw.) in Miraflores, Colombia
Authors: Morillo-Coronado Ana Cruz , Manjarres-Hernández Elsa Helena , Saenz-Quintero Óscar Javier , Morillo-Coronado Yacenia
Published: June 2022

Selenicereus megalanthus is a native fruit tree with broad phenotypic variations that has not been characterized. The objective of this research was to morphoagronomically evaluate yellow pitahaya genotypes in open fields and under cover in the municipality of Miraflores, Boyacá. A diagnostic census of the productive system was carried out. The morphoagronomic characterization used a completely random design with qualitative and quantitative descriptors for fruits and cladodes taken in situ and analyzed with frequency, descriptive, multivariate, conglomerate, and sperm correlation analyses. The pitahaya production system was based on empirical practices carried out by farmers. The weight of the largest fruit in open fields was 219.04 g on average; the average was 186.48 g with the covered system. The open-field systems had the largest genotypes in all the dimensions (length and width). The covered systems had the highest number of fruits per cladode (3.70) and the longest cladodes in the entire study (121.24 cm). Both production systems showed similar values for titratable acidity (0.20), and the soluble solids values were slightly higher in the open-field system than in the covered system (15.20 and 14.66 °Brix, respectively), desirable characteristics for the market. Genotypes 7 (under cover) and 3 (open field) presented outstanding morphological and agronomic characteristics. This study identified genotypes that can be included in selection programs for yellow pitahaya in Miraflores, Colombia.
Comparison of Pure and Crossbred Japanese Black Steers in Growth Performance and Metabolic Features from Birth to Slaughter at a Spanish Fattening Farm
Authors: Vázquez-Mosquera Juan M. , de Mercado Eduardo , Fernández-Novo Aitor , Gardón Juan C. , Pesántez-Pacheco José L. , Pérez-Solana María Luz , Revilla-Ruiz Ángel , Martínez Daniel , Villagrá Arantxa , Sebastián Francisco , Pérez-Garnelo Sonia S. , Astiz Susana
Published: June 2022

Japanese Black (Wagyu) cattle produce high-quality beef. However, whether Wagyu steers can be profitably raised under conditions different than the traditional Japanese ones remains unclear. From 2018 to 2020, we raised 262 Wagyu purebred steers, 103 Wagyu-by-Angus (Wangus) crossbred steers, and 43 Angus-by-European (ACL) crossbred steers on a Spanish farm with high welfare standards and a locally sourced, high-olein diet. Factors and factors’ interactions impacting steer growth were analyzed using generalized linear models. ACL steers grew faster than the other two groups, with Wangus showing intermediate fattening and muscle development. Average daily weight gains (kg/day) were 0.916 for Wagyu, 1.046 for Wangus, and 1.293 for ACL during the weaning to growing period, and 0.628 for Wagyu, 0.64 for Wangus, and 0.802 for ACL during the growing to fattening phase. ACL showed the lowest marbling rates. Wagyu and Wangus usually showed higher cholesterol, triglycerides, and high-density lipoprotein than ACL. ACL calves may experience greater stress at weaning, as suggested by higher glucose, lactate, and β-hydroxybutyrate than the other groups. The results suggest that Wagyu and Wangus steers showed adequate growth, health, and metabolic development in this type of production system, with Wagyu purebreds probably being more profitable than Wangus crossbreeds.
Uterine Inflammation Changes the Expression of Cholinergic Neurotransmitters and Decreases the Population of AChE-Positive, Uterus-Innervating Neurons in the Paracervical Ganglion of Sexually Mature Gilts
Authors: Miciński Bartosz , Jana Barbara , Całka Jarosław
Published: June 2022

The focus of this study was based on examining the impact of endometritis on the chemical coding of the paracervical ganglion (PCG) perikaryal populations supplying pig uterus. Four weeks after the injection of Fast Blue retrograde tracer into uterine horns, either the Escherichia coli (E. coli) suspension or saline solution was applied to both horns. Laparotomy treatment was performed for the control group. Uterine cervices containing PCG were extracted on the eighth day after previous treatments. Subsequent macroscopic and histopathologic examinations acknowledged the severe form of acute endometritis in the E. coli-treated gilts, whereas double-labeling immunofluorescence procedures allowed changes to be analyzed in the PCG perikaryal populations coded with vesicular acetylcholine transporter (VAChT) and/or somatostatin (SOM), vasoactive intestinal polypeptide (VIP), a neuronal isoform of nitric oxide synthase (nNOS), galanin (GAL). The acetylcholinesterase (AChE) detection method was used to check for the presence and changes in the expression of this enzyme and further confirm the presence of cholinergic perikarya in PCG. Treatment with E. coli resulted in an increase in VAChT+/VIP+, VAChT+/VIP−, VAChT+/SOM+, VAChT+/SOM−, VAChT+/GAL− and VAChT+/nNOS− PCG uterine perikarya. An additional increase was noted in the non-cholinergic VIP-, SOM- and nNOS-immunopositive populations, as well as a decrease in the number of cholinergic nNOS-positive perikarya. Moreover, the population of cholinergic GAL-expressing perikarya that appeared in the E. coli-injected gilts and E. coli injections lowered the number of AChE-positive perikarya. The neurochemical characteristics of the cholinergic uterine perikarya of the PCG were altered and influenced by the pathological state (inflammation of the uterus). These results may indicate the additional influence of such a state on the functioning of this organ.
New Insights into the Diurnal Rhythmicity of Gut Microbiota and Its Crosstalk with Host Circadian Rhythm
Authors: Wang Hongyu , Zhang He , Su Yong
Published: June 2022

Unlike the strictly hierarchical organization in the circadian clock system, the gut microbiota rhythmicity has a more complex multilayer network of all taxonomic levels of microbial taxa and their metabolites. However, it is worth noting that the functionality of the gut microbiota rhythmicity is highly dependent on the host circadian clock and host physiological status. Here, we discussed the diurnal rhythmicity of the gut microbiota; its crucial role in host physiology, health, and metabolism; and the crosstalk between the gut microbial rhythmicity and host circadian rhythm. This knowledge lays the foundation for the development of chronotherapies targeting the gut microbiota. However, the formation mechanism, its beneficial effects on the host of gut microbial rhythmicity, and the dynamic microbial–host crosstalk are not yet clear and warrant further research.
Coral Hydrate, a Novel Antioxidant, Improves Alcohol Intoxication in Mice
Authors: Wu Hung-Tsung , Chao Ting-Hsing , Ou Horng-Yih , Tsai Liang-Miin
Published: June 2022

Alcohol-drinking culture may cause individuals to periodically experience unpleasant hangovers. In addition, ethanol catabolism stimulates the production of free radicals that may cause liver injury and further lead to the development of chronic alcoholic fatty liver disease. Although a number of studies have suggested that hydrogenated water may be consumed to act as free radical scavenger, its instability limits its application. In this study, we used coral hydrate (i.e., hydrogenated coral materials) as a more stable hydrogen source and evaluated its effects in a murine model of alcohol intoxication. In solution, coral hydrate exhibited much more stable redox potential than did hydrogenated water. Furthermore, administration of coral hydrate by oral gavage significantly prolonged the time to fall asleep and decreased the total sleep time in mice that received intraperitoneal injection of ethanol. The mice receiving coral hydrate also had lower plasma ethanol and acetaldehyde levels than controls. In line with this observation, hepatic expression of alcohol dehydrogenase, acetaldehyde dehydrogenase, catalase and glutathione peroxidase were all significantly increased by the treatment. Meanwhile, alcohol-induced upregulation of pro-inflammatory factors was attenuated by the administration of coral hydrate. Taken together, our data suggest that coral hydrate might be an effective novel treatment for alcohol intoxication.