bluuebunny/crossref_metadata_2025 huggingface dataset:
Ginsenoside Rg1 Prevents and Treats Acute Pulmonary Injury Induced by High-Altitude Hypoxia
Authors: Chen Junru , Zhang Zhuo , Huang Mingyue , Yan Jiayi , Gao Rong , Cui Jialu , Gao Yue , Ma Zengchun
Published: November 2024

This study aimed to investigate the protective effects of ginsenoside Rg1 on high-altitude hypoxia-induced acute lung injury (ALI) and elucidated its molecular targets and related pathways, specifically its association with the fluid shear stress pathway. Using a combination of bioinformatics analysis and both in vivo and in vitro experiments, we assessed the role of ginsenoside Rg1 in mitigating physiological and biochemical disturbances induced by hypoxia. In the in vivo experiments, we measured arterial blood gas parameters, levels of inflammatory cells and cytokines, erythrocyte and platelet parameters, and conducted histological analysis in rats. The in vitro experiments utilized human pulmonary microvascular endothelial cells (HPMECs) and A549 cells to examine cell viability, intracellular reactive oxygen species (ROS) and Ca2⁺ levels, and mitochondrial function. The results of the in vivo experiments demonstrate that ginsenoside Rg1 significantly increased arterial blood oxygen partial pressure and saturation, elevated arterial blood glucose levels, and stabilized respiratory and metabolic functions in rats. It also reduced inflammatory cells and cytokines, such as tumor necrosis factor-α and interleukin-6, and improved erythrocyte and platelet abnormalities, supporting its protective role through the regulation of the fluid shear stress pathway. Histological and ultrastructural analyses revealed that Rg1 significantly protected lung tissue structure and organelles. In vitro experiments further confirmed that Rg1 improved cell viability in HPMEC and A549 cells under hypoxic conditions, decreased intracellular ROS and Ca2⁺ levels, and enhanced mitochondrial function. These findings collectively demonstrate that ginsenoside Rg1 exerts significant protective effects against high-altitude hypoxia-induced ALI by enhancing oxygen delivery and utilization, reducing inflammatory responses, and maintaining cellular metabolism and vascular function. Notably, the protective effects of Rg1 are closely associated with the regulation of the fluid shear stress pathway, suggesting its potential for treating high-altitude hypoxia-related diseases.
The Possible Roles of Glucosamine-6-Phosphate Deaminases in Ammonium Metabolism in Cancer
Authors: Lara-Lemus Roberto , Castillejos-López Manuel , Aquino-Gálvez Arnoldo
Published: November 2024

Nearly 5% of the glucose-6-phosphate (Glc6P) in cells is diverted into the hexosamine biosynthetic pathway (HBP) to synthesize glucosamine-6-phosphate (GlcN6P) and uridine diphosphate N-acetyl-glucosamine-6-phosphate (UDP-GlcN6P). Fructose-6-phosphate (Fru6P) is a common intermediary between glycolysis and the HBP. Changes in HBP regulation cause abnormal protein N-glycosylation and O-linked-N-acetylglucosamine modification (O-GlcNAcylation), affecting protein function and modifying cellular responses to signals. The HBP enzymes glucosamine-6-phosphate deaminases 1 and 2 (GNPDA1 and 2) turn GlcN6P back into Fru6P and ammonium, and have been implicated in cancer and metabolic diseases. Despite the plentiful literature on this topic, the mechanisms involved are just beginning to be studied. In this review, we summarize, for the first time, the current knowledge regarding the possible roles of the isoenzymes of both GNPDAs in the pathogenesis and development of metabolic diseases and cancer from a molecular point of view, highlighting their importance not only in supplying carbon from glycolysis, but also in ammonia metabolism.
Structural and Functional Biology of Mammalian ALOX Isoforms with Particular Emphasis on Enzyme Dimerization and Their Allosteric Properties
Authors: Zhuravlev Alexander , Gavrilyuk Viktor , Chen Xin , Aksenov Vladislav , Kuhn Hartmut , Ivanov Igor
Published: November 2024

The human genome involves six functional arachidonic acid (AA) lipoxygenase (ALOX) genes, and the corresponding enzymes (ALOX15, ALOX15B, ALOX12, ALOX12B, ALOXE3, ALOX5) have been implicated in cell differentiations and in the pathogenesis of inflammatory, hyperproliferative, metabolic, and neurological disorders. Humans express two different AA 15-lipoxygenating ALOX isoforms, and these enzymes are called ALOX15 (15-LOX1) and ALOX15B (15-LOX2). Chromosomal localization, sequence alignments, and comparison of the enzyme properties suggest that pig and mouse ALOX15 orthologs (leukocyte-type 12-LOX) on the one hand and rabbit and human ALOX15 orthologs on the other (reticulocyte-type 15-LOX1) belong to the same enzyme family despite their different reaction specificities with AA as a substrate. In contrast, human ALOX12 (platelet-type 12-LOX), as well as pig and mouse ALOX15 (leukocyte-type 12-LOX), belong to different enzyme families, although they exhibit a similar reaction specificity with AA as a substrate. The complex multiplicity of mammalian ALOX isoforms and the controversial enzyme nomenclatures are highly confusing and prompted us to summarize the current knowledge on the biological functions, enzymatic properties, and allosteric regulation mechanisms of mammalian ALOX15, ALOX15B, and ALOX12 orthologs that belong to three different enzyme sub-families.
Kallmann Syndrome: Functional Analysis of a CHD7 Missense Variant Shows Aberrant RNA Splicing
Authors: Carriço Josianne Nunes , Gonçalves Catarina Inês , Aragüés José Maria , Lemos Manuel Carlos
Published: November 2024

Kallmann syndrome is a rare disorder characterized by hypogonadotropic hypogonadism and an impaired sense of smell (anosmia or hyposmia) caused by congenital defects in the development of the gonadotropin-releasing hormone (GnRH) and olfactory neurons. Mutations in several genes have been associated with Kallmann syndrome. However, genetic testing of this disorder often reveals variants of uncertain significance (VUS) that remain uninterpreted without experimental validation. The aim of this study was to analyze the functional consequences of a heterozygous missense VUS in the CHD7 gene (c.4354G>T, p.Val1452Leu), in a patient with Kallmann syndrome with reversal of hypogonadism. The variant, located in the first nucleotide of exon 19, was analyzed using minigene assays to determine its effect on ribonucleic acid (RNA) splicing. These showed that the variant generates two different transcripts: a full-length transcript with the missense change (p.Val1452Leu), and an abnormally spliced transcript lacking exon 19. The latter results in an in-frame deletion (p.Val1452_Lys1511del) that disrupts the helicase C-terminal domain of the CHD7 protein. The variant was reclassified as likely pathogenic. These findings demonstrate that missense variants can exert more extensive effects beyond simple amino acid substitutions and underscore the critical role of functional analyses in VUS reclassification and genetic diagnosis.
The Carcinogenesis of the Human Scalp: An Immunometabolic-Centered View
Authors: Kaplan Baruch , von Dannecker Rebecca , Arbiser Jack L.
Published: November 2024

The human scalp is a common site of skin cancer in humans, with nonmelanoma skin cancer being exceedingly common. In this review, two dermatologists with extensive experience in cutaneous oncology will discuss unique features of the epidemiology of cancer of the scalp. Clinical observations on these common skin cancers lead to insight into the pathogenesis and potential prevention and treatment of cutaneous scalp neoplasia. Our hypothesis is that the presence of hair protects against the development of skin cancer but not by serving as a physical shield but rather by providing continuous IL-17-biased immunosurveillance. The loss of hair allows for a release from immunosurveillance, resulting in the expansion of neoplastic cells towards skin cancer. Both hair follicles and metabolic changes in stroma allow for permissiveness for tumor promotion.
A New Probiotic Formulation Promotes Resolution of Inflammation in a Crohn’s Disease Mouse Model by Inducing Apoptosis in Mucosal Innate Immune Cells
Authors: De Salvo Carlo , Osme Abdullah , Ghannoum Mahmoud , Cominelli Fabio , Di Martino Luca
Published: November 2024

The interaction between gut-residing microorganisms plays a critical role in the pathogenesis of Crohn’s disease (CD), where microbiome dysregulation can alter immune responses, leading to unresolved local inflammation. The aim of this study is to analyze the immunomodulatory properties of a recently developed probiotic + amylase blend in the SAMP1/YitFc (SAMP) mouse model of CD-like ileitis. Four groups of SAMP mice were gavaged for 56 days with the following treatments: 1) probiotic strains + amylase (0.25 mg/100 µL PBS); 2) only probiotics; 3) only amylase; PBS-treated controls. Ilea were collected for GeoMx Digital Spatial Profiler (DSP) analysis and histological evaluation. Histology assessment for inflammation indicated a significantly reduced level of ileitis in mice administered the probiotics + amylase blend. DSP analysis showed decreased abundance of neutrophils and increased abundance of dendritic cells, regulatory T cells, and macrophages, with a significant enrichment of five intracellular pathways related to apoptosis, in probiotics + amylase-treated mice. Increased apoptosis occurrence was confirmed by (TdT)- deoxyuridine triphosphate (dUTP)-biotin nick end labeling assay. Our data demonstrate a beneficial role of the probiotic and amylase blend, highlighting an increased apoptosis of innate immunity-associated cell subsets, thus promoting the resolution of inflammation. Hence, we suggest that the developed probiotic enzyme blend may be a therapeutic tool to manage CD and therefore is a candidate formulation to be tested in clinical trials.
Can Novel Biomarkers Effectively Predict Acute Kidney Injury in Liver or Kidney Transplant Recipients?
Authors: Zywno Hubert , Figiel Wojciech , Grat Michal , Nazarewski Slawomir , Galazka Zbigniew , Malyszko Jolanta
Published: November 2024

Acute kidney injury (AKI) constitutes a common complication associated with liver or kidney transplantation, which may significantly impact the graft condition and perioperative mortality. Current AKI diagnostic criteria based on serum creatinine (sCr) and urine output alterations are widely utilized in routine clinical practice. However, the diagnostic value of sCr may be limited by various confounding factors, including age, sex, reduced or increased muscle mass, and pre-existing chronic kidney disease (CKD). Furthermore, sCr is rather a late indicator of AKI, as its concentration tends to increase only when the severity of the injury is enough to decrease the estimated glomerular filtration rate (eGFR). Recent expertise highlights the need for novel biomarkers in post-transplantation AKI diagnosis, prediction of event-associated mortality, or evaluation of indications for renal replacement treatment (RRT). Over the last decade, the diagnostic performance of various AKI biomarkers has been assessed, among which some showed the potential to outperform sCr in AKI diagnosis. Identifying susceptible individuals, early diagnosis, and prompt intervention are crucial for successful transplantation, undisturbed graft function in long-term follow-up, and decreased mortality. However, the research on AKI biomarkers in transplantation still needs to be explored. The field lacks consistent results, rigorous study designs, and external validation. Considering the rapidly growing prevalence of CKD and cirrhosis that are associated with the transplantation at their end-stage, as well as the existing knowledge gap, the aim of this article was to provide the most up-to-date review of the studies on novel biomarkers in the diagnosis of post-transplantation AKI.
Unraveling Macrophage Polarization: Functions, Mechanisms, and “Double-Edged Sword” Roles in Host Antiviral Immune Responses
Authors: Yao Meng , Li Meilin , Peng Dingkun , Wang Yijing , Li Su , Zhang Ding , Yang Bo , Qiu Hua-Ji , Li Lian-Feng
Published: November 2024

Numerous viruses that propagate through the respiratory tract may be initially engulfed by macrophages (Mφs) within the alveoli, where they complete their first replication cycle and subsequently infect the adjacent epithelial cells. This process can lead to significant pathological damage to tissues and organs, leading to various diseases. As essential components in host antiviral immune systems, Mφs can be polarized into pro-inflammatory M1 Mφs or anti-inflammatory M2 Mφs, a process involving multiple signaling pathways and molecular mechanisms that yield diverse phenotypic and functional features in response to various stimuli. In general, when infected by a virus, M1 macrophages secrete pro-inflammatory cytokines to play an antiviral role, while M2 macrophages play an anti-inflammatory role to promote the replication of the virus. However, recent studies have shown that some viruses may exhibit the opposite trend. Viruses have evolved various strategies to disrupt Mφ polarization for efficient replication and transmission. Notably, various factors, such as mechanical softness, the altered pH value of the endolysosomal system, and the homeostasis between M1/M2 Mφs populations, contribute to crucial events in the viral replication cycle. Here, we summarize the regulation of Mφ polarization, virus-induced alterations in Mφ polarization, and the antiviral mechanisms associated with these changes. Collectively, this review provides insights into recent advances regarding Mφ polarization in host antiviral immune responses, which will contribute to the development of precise prevention strategies as well as management approaches to disease incidence and transmission.
SARS-CoV-2 Infection and Alpha-Synucleinopathies: Potential Links and Underlying Mechanisms
Authors: Motyl Joanna Agata , Gromadzka Grażyna , Czapski Grzegorz Arkadiusz , Adamczyk Agata
Published: November 2024

Alpha-synuclein (α-syn) is a 140-amino-acid, intrinsically disordered, soluble protein that is abundantly present in the brain. It plays a crucial role in maintaining cellular structures and organelle functions, particularly in supporting synaptic plasticity and regulating neurotransmitter turnover. However, for reasons not yet fully understood, α-syn can lose its physiological role and begin to aggregate. This altered α-syn disrupts dopaminergic transmission and causes both presynaptic and postsynaptic dysfunction, ultimately leading to cell death. A group of neurodegenerative diseases known as α-synucleinopathies is characterized by the intracellular accumulation of α-syn deposits in specific neuronal and glial cells within certain brain regions. In addition to Parkinson’s disease (PD), these conditions include dementia with Lewy bodies (DLBs), multiple system atrophy (MSA), pure autonomic failure (PAF), and REM sleep behavior disorder (RBD). Given that these disorders are associated with α-syn-related neuroinflammation—and considering that SARS-CoV-2 infection has been shown to affect the nervous system, with COVID-19 patients experiencing neurological symptoms—it has been proposed that COVID-19 may contribute to neurodegeneration in PD and other α-synucleinopathies by promoting α-syn misfolding and aggregation. In this review, we focus on whether SARS-CoV-2 could act as an environmental trigger that facilitates the onset or progression of α-synucleinopathies. Specifically, we present new evidence on the potential role of SARS-CoV-2 in modulating α-syn function and discuss the causal relationship between SARS-CoV-2 infection and the development of parkinsonism-like symptoms.
Mining of Candidate Genes Associated with Leaf Shape Traits in Grapes
Authors: Zhang Chuan , Yadav Vivek , Cui Liwen
Published: November 2024

As the most important organ for photosynthesis, leaves provide the main energy source for plant growth. Leaf traits affect light energy utilization and, thus, plant development and biomass. Given the high morphological variability of leaves between and within grape genotypes, phenotypic analysis is challenging. This study first evaluated leaf shape trait parameters using a specific leaf profile and area analyzer, along with genome-wide association study (GWAS) analyses, to identify additional candidate genes related to grape leaf shape traits. In the two-year analysis, 89 single-nucleotide polymorphisms (SNPs) were found to be significantly associated with leaf shape traits. These SNP loci were distributed on 18 chromosomes, of which chromosome 15 had the most relevant SNPs. We found that leaf shape-associated genes included mainly plant hormone-, ubiquitin ligase-, serine/threonine protein kinase-, transcription factor-, and cell wall metabolism-related genes. By analyzing the expression of these candidate genes on the chip, we found that they exhibited diverse expression levels in leaves at different developmental stages (young, mature, and senescent). This suggests that these genes could be considered candidates for grape leaf improvement.
Comprehensive Characterization of Anti-HLA and Non-HLA Antibodies in Patients on Kidney Transplant Waiting List and Evaluation of Their Impact on Alloimmunization Risk and Dialysis Treatment
Authors: Mujić Franić Aida , Lilić Marko , Katalinić Nataša , Glavaš-Obrovac Ljubica
Published: November 2024

Alloimmunization remains a major obstacle to successful kidney transplantation, mainly due to the formation of anti-HLA antibodies. In recent years, non-HLA antibodies have emerged as additional immunologic factors that can potentially contribute to graft rejection. The aim of this study was to investigate the prevalence and specificity of both anti-HLA and non-HLA antibodies in patients with end-stage renal disease on a waiting list for kidney transplantation. Serum samples from 74 patients were analyzed using complement-dependent cytotoxicity and solid-phase assays. IgG anti-HLA antibodies were identified in 43.2% of participants, while IgG non-HLA antibodies were detected in 91.9%. The most frequent non-HLA antibodies included anti-ENO1 (28.4%), anti-FIBR1 (23.0%) and anti-PRKCZ (23.0%). A significant difference was found between the number of distinct IgG anti-HLA and IgG non-HLA antibody specificities. However, no significant correlation was found between the number of IgG non-HLA antibody specificities and previous alloimmunization events or dialysis treatments. These results suggest that non-HLA antibodies, although often overlooked, can sometimes play a critical role in transplant outcomes. Routine testing for non-HLA antibodies, in addition to mandatory anti-HLA antibody screening and identification, could improve immunologic risk assessment in transplant patients and post-transplant care.
Cytokines and Madness: A Unifying Hypothesis of Schizophrenia Involving Interleukin-22
Authors: Sfera Adonis , Thomas Kyle A. , Anton Jacob
Published: November 2024

Schizophrenia is a severe neuropsychiatric illness of uncertain etiopathogenesis in which antipsychotic drugs can attenuate the symptoms, but patients rarely return to the premorbid level of functioning. In fact, with each relapse, people living with schizophrenia progress toward disability and cognitive impairment. Moreover, our patients desire to live normal lives, to manage their daily affairs independently, date, get married, and raise and support a family. Those of us who work daily with schizophrenia patients know that these objectives are rarely met despite the novel and allegedly improved dopamine blockers. We hypothesize that poor outcomes in schizophrenia reflect the gray matter volume reduction, which continues despite antipsychotic treatment. We hypothesize further that increased gut barrier permeability, due to dysfunctional aryl hydrocarbon receptor (AhR), downregulates the gut barrier protectors, brain-derived neurotrophic factor (BDNF), and interleukin-22 (IL-22), facilitating microbial translocation into the systemic circulation, eventually reaching the brain. Recombinant human IL-22 could ameliorate the outcome of schizophrenia by limiting bacterial translocation and by initiating tissue repair. This short review examines the signal transducer and transcription-three (STAT3)/AhR axis and downregulation of IL-22 and BDNF with subsequent increase in gut barrier permeability. Based on the hypothesis presented here, we discuss alternative schizophrenia interventions, including AhR antagonists, mitochondrial transplant, membrane lipid replacement, and recombinant human IL-22.
A Systematic Review of Dietary Lifestyle Interventions for Neuropathic Pain
Authors: Klowak Michael , Lau Rachel , Mohammed Mariyam N. , Birago Afia , Samson Bethel , Ahmed Layla , Renee Camille , Meconnen Milca , Sam Mahmud , Boggild Andrea K.
Published: November 2024

Background/Objectives: Chronic severe neuropathic pain (NP) affects one in 10 individuals over the age of 30 in North America. Standard pharmacological interventions are associated with significant side effects and have limited effectiveness. Diets seeking to improve physiological health, support gut barrier integrity, and decrease systemic inflammation have recently emerged as powerful tools conferring neuroprotective and anti-inflammatory effects, potentially reducing the overall morbidity and mortality of multiple neurological and metabolic diseases. This systematic review aimed to synthesize the literature around NP outcomes following dietary interventions compared to routine standard of care. Methods: Following PRISMA guidelines, an initial search yielded 15,387 records after deduplication. Six interventional trials specifically assessing dietary interventions for neuropathic pain were included and analyzed. The dietary lifestyle interventions included low-fat plant-based, plant-based fasting-mimicking, low-calorie, potassium-reduced, gluten-free, and intermittent high-protein/Mediterranean diets. Results: The included studies described some statistically significant improvements in pain severity on objective quantitative sensory testing, electrophysiology, imaging, and subjective questionnaires. The overall risk of bias was moderate, with only one trial demonstrating a low risk of bias across all assessed domains. No serious adverse events were identified, and dietary interventions were generally well tolerated. Conclusions: The data collected and synthesized in this systematic review indicate that dietary lifestyle interventions may offer a low-risk, low-cost, low-tech option for chronic neuropathic pain management, potentially improving quality of life and reducing overall morbidity. However, given substantial variability across studies and a moderate risk of bias, further research is warranted to substantiate these findings.
Intraocular Pressure Reduction Following Phacoemulsification in Patients with Exfoliation: A Systematic Review and Meta-Analysis
Authors: Benekos Konstantinos , Katsanos Andreas , Laspas Panagiotis , Vagiakis Iordanis , Haidich Anna-Bettina , Konstas Anastasios G.
Published: November 2024

Objectives: The objective of this systematic review and meta-analysis is to evaluate the existing evidence and estimate the impact of phacoemulsification and intraocular lens implantation on the intraocular pressure (IOP) of subjects with exfoliation syndrome (XFS) or exfoliative glaucoma (XFG). Methods: In July 2024, an in-depth literature review across three databases was undertaken. This study focused only on adult patients with exfoliation who had not undergone previous ocular surgery. The primary outcome of interest was the mean IOP reduction at 6 and 12 months after uncomplicated phacoemulsification surgery. Results: This meta-analysis included one randomized controlled trial and eight observational studies, comprising 220 patients at 6 months and 430 patients at the 12-month time point, respectively. The mean IOP reduction after surgery was 3.43 mmHg (95% CI: −4.77 to −2.09) after 6 months and 2.75 mmHg (95% CI: −4.24 to −1.26) after 12 months. In both time points, there was no heterogeneity (I2 = 0), but the certainty of evidence following the GRADE evaluation was very low. Conclusions: The present meta-analysis demonstrates that phacoemulsification can significantly reduce IOP in exfoliation subjects 6 and 12 months after surgery. Nevertheless, significant limitations in included studies do not allow a precise and certain estimate of the magnitude of postoperative IOP lowering in exfoliation patients. Additional research is needed to confirm these results.
Effect of Mechanically Exfoliated Graphite Flakes on Morphological, Mechanical, and Thermal Properties of Epoxy
Authors: Gül Ayşenur , Kamali Ali Reza
Published: November 2024

Carbon-reinforced polymer composites form an important category of advanced materials, and there is an increasing demand to enhance their performance using more convenient and scalable processes at low costs. In the present study, graphitic flakes were prepared by the mechanical exfoliation of synthetic graphite electrodes and utilized as an abundant and potentially low-cost filler to fabricate epoxy-based composites with different additive ratios of 1–10 wt.%. The morphological, structural, thermal, and mechanical properties of these composites were investigated. It was found that the thermal conductivity of the composites increases by adding graphite, and this increase mainly depends on the ratio of the graphite additive. The addition of graphite was found to have a diverse effect on the mechanical properties of the composites: the tensile strength of the composites decreases with the addition of graphite, whilst their compressive strength and elastic modulus are enhanced. The results demonstrate that incorporating 5 wt% of commercially available graphite into epoxy not only raises the thermal conductivity of the material from 0.223 to 0.485 W/m·K, but also enhances its compressive strength from 66 MPa to 72 MPa. The diverse influence of graphite provides opportunities to prepare epoxy composites with desirable properties for different applications.
Comparative Study of Random Forest and Support Vector Machine for Land Cover Classification and Post-Wildfire Change Detection
Authors: Tan Yan-Cheng , Duarte Lia , Teodoro Ana Cláudia
Published: November 2024

The land use land cover (LULC) map is extensively employed for different purposes. Machine learning (ML) algorithms applied in remote sensing (RS) data have been proven effective in image classification, object detection, and semantic segmentation. Previous studies have shown that random forest (RF) and support vector machine (SVM) consistently achieve high accuracy for land classification. Considering the important role of Portugal’s Serra da Estrela Natural Park (PNSE) in biodiversity and nature conversation at an international scale, the availability of timely data on the PNSE for emergency evaluation and periodic assessment is crucial. In this study, the application of RF and SVM classifiers, and object-based (OBIA) and pixel-based (PBIA) approaches, with Sentinel-2A imagery was evaluated using Google Earth Engine (GEE) platform for the land cover classification of a burnt area in the PNSE. This aimed to detect the land cover change and closely observe the burnt area and vegetation recovery after the 2022 wildfire. The combination of RF and OBIA achieved the highest accuracy in all evaluation metrics. At the same time, a comparison with the Normalized Difference Vegetation Index (NDVI) map and Conjunctural Land Occupation Map (COSc) of 2023 year indicated that the SVM and PBIA map resembled the maps better.
Enhancement of Magnetic Shielding Based on Low-Noise Materials, Magnetization Control, and Active Compensation: A Review
Authors: Liu Yijin , Yang Jianzhi , Cao Fuzhi , Zhang Xu , Zheng Shiqiang
Published: November 2024

Magnetic-shielding technologies play a crucial role in the field of ultra-sensitive physical measurement, medical imaging, quantum sensing, etc. With the increasing demand for the accuracy of magnetic measurement, the performance requirements of magnetic-shielding devices are also higher, such as the extremely weak magnetic field, gradient, and low-frequency noise. However, the conventional method to improve the shielding performance by adding layers of materials is restricted by complex construction and inherent materials noise. This paper provides a comprehensive review about the enhancement of magnetic shielding in three aspects, including low-noise materials, magnetization control, and active compensation. The generation theorem and theoretical calculation of materials magnetic noise is summarized first, focusing on the development of spinel ferrites, amorphous, and nanocrystalline. Next, the principles and applications of two magnetization control methods, degaussing and magnetic shaking, are introduced. In the review of the active magnetic compensation system, the forward and inverse design methods of coil and the calculation method of the coupling effect under the ferromagnetic boundary of magnetic shield are explained in detail, and their applications, especially in magnetocardiography (MCG) and magnetoencephalogram (MEG), are also mainly described. In conclusion, the unresolved challenges of different enhancement methods in materials preparation, optimization of practical implementation, and future applications are proposed, which provide comprehensive and instructive references for corresponding research.
Tests of Uniaxial Compression of Single Grains
Authors: Radosz Iwona , Pietrzak Magdalena , Kaczmarek Leszek M.
Published: November 2024

Tests of the uniaxial compression of single grains were performed in a specially designed press, which allowed the recording of an applied load in regard to the time and observation of occurring phenomena in a polarization assay. Three types of grains were tested: quartz sand, glass granules, and crushed glass. The strength tests showed different mechanisms of grain damage depending on the type of grain. In addition, the formation and spread of interference fringes, forming “chains of force” in samples with a large number of grains, were observed by testing glass grains under the polarization assay. A more detailed understanding of the strength characteristics of single grains will allow the verification of the models most commonly used in DEM.
Penalty 4-Node Quadrilateral Element Formulation for Axisymmetric Couple Stress Problems
Authors: Jiang Yongkang , Shang Yan
Published: November 2024

To address the issue of size effects in axisymmetric deformation of small-scale solids, this work proposes a 4-node 12-DOF element for axisymmetric problems based on the consistent couple stress theory (CCST), following the framework of the unsymmetric finite element method. With the use of the penalty function method, an independently assumed rotational field is introduced into the virtual work principle to approximate the physical rotation, ensuring the satisfaction of the C1 continuity requirement of the CCST in a weak form. As a benefit, the enriched C0 isoparametric-based interpolation is employed to construct the test functions for displacement and rotation. Furthermore, the force-stress field that satisfies the equilibrium equations related to axisymmetric deformation is employed as the element’s force-stress trial function. In order to circumvent locking issues, reduced integration is employed in the penalty stiffness integration process. The numerical results demonstrate that the new element exhibits high computational accuracy and convergence rate in both static and modal analysis problems, effectively capturing size-dependent phenomena.
An Experimental Comparison of Self-Adaptive Differential Evolution Algorithms to Induce Oblique Decision Trees
Authors: Rivera-López Rafael , Mezura-Montes Efrén , Canul-Reich Juana , Cruz-Chávez Marco-Antonio
Published: November 2024

This study addresses the challenge of generating accurate and compact oblique decision trees using self-adaptive differential evolution algorithms. Although traditional decision tree induction methods create explainable models, they often fail to achieve optimal classification accuracy. To overcome these limitations, other strategies, such as those based on evolutionary computation, have been proposed in the literature. In particular, we evaluate the use of self-adaptive differential evolution variants to evolve a population of oblique decision trees encoded as real-valued vectors. Our proposal includes (1) an alternative initialization strategy that reduces redundant nodes and (2) a fitness function that penalizes excessive leaf nodes, promoting smaller and more accurate decision trees. We perform a comparative performance analysis of these differential evolution variants, showing that while they exhibit similar statistical behavior, the Single-Objective real-parameter optimization (jSO) method produces the most accurate oblique decision trees and is second best in compactness. The findings highlight the potential of self-adaptive differential evolution algorithms to improve the effectiveness of oblique decision trees in machine learning applications.
Association Between Sarcopenia and Acupressure Testing in Older Adults Requiring Long-Term Care
Authors: Shiba Takahiro , Sawaya Yohei , Sato Ryo , Hirose Tamaki , Yin Lu , Shiraki Masataka , Ishizaka Masahiro , Kubo Akira , Urano Tomohiko
Published: November 2024

Background and Objectives: This study analyzed the relationship between pressure pain test outcomes and sarcopenia in elderly patients and explored possible clinical applications. Materials and Methods: The participants included 143 older adults requiring long-term care who could be diagnosed with sarcopenia. Along with sarcopenia diagnosis, the participants underwent acupressure testing symmetrically at nine sites (occiput, lower cervical, trapezius, supraspinatus, second rib, lateral epicondyle, gluteus, greater trochanter, and knee), totaling 18 sites. The analyses included comparisons of sarcopenia status and total tender points between the groups and a multivariable analysis. The association between sarcopenia and the number of tender points were examined based on correlations between the number of tender points and grip strength, walking speed, and skeletal muscle mass index (SMI). Intergroup comparisons and multivariable analysis of tender points with and without sarcopenia were performed to investigate specific tender points associated with sarcopenia. Results: An independent association was observed between sarcopenia and the number of tender points (p = 0.001). Furthermore, the number of tender points was correlated with grip strength (ρ = −0.536, p < 0.001), walking speed (ρ = −0.200, p = 0.028), and SMI (ρ = −0.394, p < 0.001). The supraspinatus (p = 0.029, 95% confidence interval: 1.221–35.573) and lower cervical (p = 0.039, 95% confidence interval: 1.050–7.245) regions were identified as specific tender points. Conclusions: In older adult patients requiring long-term care, sarcopenia is associated with an increased number of tender points throughout the body, with the supraspinatus and lower cervical regions potentially being specific tender points. Acupressure testing for tenderness may be a useful assessment parameter in sarcopenia patients.
Enhancing the Separation Performance of Chitosan Membranes Through the Blending with Deep Eutectic Solvents for the Pervaporation of Polar/Non-Polar Organic Mixtures
Authors: Galiano Francesco , Msahel Asma , Russo Francesca , Rovella Natalia , Policicchio Alfonso , Hamouda Sofiane Ben , Hafiane Amor , Castro-Muñoz Roberto , Figoli Alberto
Published: November 2024

This study explores the development of chitosan-based membranes blended with three distinct deep eutectic solvents (DESs) for the pervaporation separation of methanol and methyl tert-butyl ether. DESs were selected for their eco-friendly properties and their potential to enhance membrane performance. The chitosan (CS) membranes, both crosslinked and non-crosslinked, were characterized in terms of morphology, chemical composition, wettability, mechanical resistance, and solvent uptake. Pervaporation tests revealed that incorporating DESs significantly enhanced the membranes’ selective permeability toward methanol, with up to a threefold increase in separation efficiency compared to pristine CS membranes. The membranes demonstrated a strong dependence on feed temperature, with higher temperatures improving permeation flux but reducing separation factor. Crosslinking with glutaraldehyde further increased membrane selectivity by reducing free volume into the polymer matrix. These findings underscore the potential of DESs as green additives for improving the performance of biopolymer membranes, making them promising candidates for efficient and eco-friendly organic–organic separations.
Metallic Metamaterials with Auxetic Properties: Re-Entrant Structures
Authors: Plewa Julian , Płońska Małgorzata , Junak Grzegorz
Published: November 2024

The present article is an exploration of metamaterial structures exhibiting auxetic properties. The study shows the effect of three geometric parameters of re-entrant auxetic cells, namely, the internal initial cell angle (θ0), the strut length ratio h/l, and the degree of opening of the unit cells expressed by the change in the Δθ angle, on the value of the Poisson’s ratio. It combines theoretical insights into physical re-entrant auxetic structures with the demonstration of structures that can be subjected to cyclic loading without being damaged. The experimental section features the results of the compression tests of a symmetrical structure made up of four re-entrant cells and tensile tests of a flat mesh structure of size 4 × 4. In the mesh structure, a modification was applied to the re-entrant cells, creating arched strut connections. It was shown that the value of the maximum load for such structures depends on the bending angle and the length of the inclined strut. The mesh structure was created using torsion springs. Its cyclic tension for different amplitudes yielded Poisson’s ratio values in the range of −1.4 to −1.7. These modifications have enabled stable, elastic, and failure-free cyclical changes of the structure’s dimensions under load.
Electron Beam Additive Manufacturing of SS316L with a Stochastic Scan Strategy: Microstructure, Texture Evolution, and Mechanical Properties
Authors: Chaithanya Kumar K. N. , Sharma Shashank , Radhakrishnan Madhavan , Randhavan Rohit , Verma Krishna Kamlesh , Dowden Shelden , Hughes Zane Weldon , Banerjee Rajarshi , Dahotre Narendra B.
Published: November 2024

This study examines the microstructure, crystallographic texture evolution, and mechanical properties of stainless steel 316L fabricated through electron beam melting using a stochastic scan strategy at a preheat temperature of 1123 K. X-ray diffraction confirmed the presence of a pure austenitic phase in the fabricated material. Equiaxed cellular structures were observed in the center of the melt pool regions and elongated cellular structures observed at the melt pool overlap regions. A finite element-based numerical model was employed to estimate the thermal gradients and solidification rates within the melt pool of an electron beam spot. Microstructural analysis indicated a generation of columnar grains from the bottom to the top of the build owing to high thermal gradients. A crystallographic texture investigation showed a generation of strong <110> fiber texture along the build direction of the material and reported that the stress distributions within the melt pool led to a strong crystallographic texture driven by the stress evolution observed from thermokinetic computational modelling of the electron beam-melting process. Mechanical properties were assessed using profilometry-based indentation plastometry, demonstrating strain hardening at a high temperature of 773 K.
Granular Soils and Contaminant Modeling in Tailing Dams
Authors: Farhadian Hadi , Jodeiri Shokri Behshad , Mirzaghorbanali Ali
Published: November 2024

The granular soils of tailings, encompassing clay, gravel, sand, and silt, play a pivotal role in the behavior and stability of tailings dams. Different types of granular soils significantly influence the tailings material’s strength, compressibility, and permeability. This study highlights the importance of understanding the relationship between soil types and contaminant properties when analyzing solute transport through numerical modeling. Consequently, various soil types were incorporated into the initial tailings dam model to simulate contaminant transport based on solute transport analysis. The findings underscored the essential role of granular soils in contaminant dispersion within tailings dams. Finer particles, such as clay and silt, demonstrated higher adsorption capacities, which slow contaminant movement. In contrast, coarser materials, like sand and gravel, enable faster transport, increasing the potential for rapid dispersion.
Sodium-Glucose Cotransporter 2 Inhibitors Improve Body Composition by Increasing the Skeletal Muscle Mass/Fat Mass Ratio in Patients with Type 2 Diabetes: A 52-Week Prospective Real-Life Study
Authors: Volpe Sara , Vozza Alfredo , Lisco Giuseppe , Fanelli Margherita , Racaniello Davide , Bergamasco Alessandro , Triggiani Domenico , Pierangeli Giulia , De Pergola Giovanni , Tortorella Cosimo , Moschetta Antonio , Piazzolla Giuseppina
Published: November 2024

Background: Sodium-glucose cotransporter 2 inhibitors (SGLT2is) induce body weight loss, but their effect on skeletal muscle mass (SMM) and strength needs to be better elucidated. Objectives: This study aimed to evaluate the effects of SGLT2i on SMM in a real-life population setting of patients with type 2 diabetes (T2D). Secondary outcomes included changes in liver steatosis and in anthropometric and glucometabolic parameters. Methods: Seventy-one patients were treated with SGLT2is as an add-on to metformin for 52 consecutive weeks. Visits were scheduled at baseline (T0) and after 6 (T6) and 12 months of therapy (T12) and included the checking of laboratory tests, measurement of anthropometric parameters, bioimpedance analysis of body composition, and abdominal ultrasound (US). Results: Fat mass (FM) and visceral adipose tissue (VAT) progressively decreased compared to the baseline (FM: −2.9 ± 0.6 kg at T6; −2.8 ± 0.6 kg at T12; VAT: −0.3 ± 0.1 L at T6; −0.4 ± 0.1 L at T12; all p < 0.01). Changes in SMM were less pronounced (−0.4 ± 0.3 kg at T6, ns; −0.7 ± 0.4 kg at T12, p < 0.05), yielding a beneficial increase in the SMM/FM ratio (+0.3 ± 0.05 at T6 and +0.2 ± 0.05 at T12, all p < 0.01). No significant changes in sarcopenia, sarcopenic obesity, fat-free mass, muscle strength, and water compartments were observed at the end of the follow-up period. Anthropometric and glucometabolic parameters, insulin resistance, liver enzymes, and biometric indices and US grading of hepatic steatosis improved throughout this study. Conclusions: In a real-life setting, SGLT2i therapy is associated with weight loss attributable to FM rather than SMM loss without any relevant deterioration in muscle strength. In addition, SGLT2is proved to have beneficial effects on steatotic liver disease.
Effectiveness of Three Front-of-Pack Food Labels in Guiding Consumer Identification of Nutrients of Concern and Purchase Intentions in Kenya: A Randomized Controlled Trial
Authors: Mohamed Shukri F. , Karugu Caroline H. , Iddi Samuel , Ojiambo Veronica , Kirui Caliph , Asiki Gershim
Published: November 2024

Background: Front-of-pack-labels (FOPLs) on packaged foods provide essential information to help consumers make informed dietary choices. However, evidence on their effectiveness, particularly in low- and middle-income countries like Kenya, is limited. Objective: This study assessed the effectiveness of three FOPLs in helping consumers identify nutrients of concern in packaged food products and influencing their purchase intention in Kenya. Methods: A total of 2198 shoppers from supermarkets in Nairobi, Mombasa, Kisumu, and Garissa were randomized into three groups: Red and Green Octagon label (RG), Red and Green Octagon with icons (RGI), and Black Octagon Warning label (WL). In the control phase, participants were shown unlabeled images of packaged foods, followed by questions. In the experimental phase, the same images were presented with one assigned FOPL, and participants responded again to the same set of questions. Differences in correct identification of nutrients of concern and changes in purchase intention were analyzed using frequency tables and Chi-Square tests, while modified Poisson regression assessed FOPL effectiveness. Results: FOPLs significantly improved correct nutrient identification and reduced the intention to purchase unhealthy foods, with the WL proving most effective. Conclusions: These findings highlight the potential of FOPLs, particularly the WL, as an effective regulatory tool for promoting healthier food choices in Kenya.
Body Composition in Cases with Normal Alanine Aminotransferase Values in Medical Health Checkups
Authors: Ushiro Kosuke , Fukuda Akira , Matsui Masahiro , Onishi Saori , Nishikawa Tomohiro , Asai Akira , Kim Soo Ki , Nishikawa Hiroki
Published: November 2024

Background and aims: We aimed to clarify the relationship between alanine aminotransferase (ALT) level and body composition in Japanese medical health checkups, especially in cases with ALT ≤ 30 IU/L (7569 men and 9497 women). Methods: We categorized our study cohort into four groups: type A (ALT value ≤ 10 IU/L), type B (11 ≤ ALT value ≤ 20 IU/L), type C (21 ≤ ALT value ≤ 30 IU/L) and type D (ALT value > 30 IU/L (ALT over 30)). We retrospectively compared body composition-related parameters (body mass index (BMI), waist circumference (WC), fat (F) index, fatty liver index (FLI), fat-free (FF) index and F-FF ratio) among the four types. Results: Type A/B/C/D in men and women was found in 262/3279/2107/1921 and 1549/5736/1495/717 (p < 0.0001). BMI, WC, F-index, FLI, FF index and F-FF ratio were all significantly stratified among the four types, regardless of whether they were male or female and over or under 50 years old. Conclusions: With a decrease in ALT level in medical health checkups, fat mass decreases, and F-FF ratio decreases, but a decrease in skeletal muscle mass cannot be overlooked.
Use of Carbohydrate (CHO), Gluten-Free, and FODMAP-Free Diets to Prevent Gastrointestinal Symptoms in Endurance Athletes: A Systematic Review
Authors: Montero-Carrasco Karen , Arias-Tellez Maria Jose , Soto-Sánchez Johana
Published: November 2024

Background: Gastrointestinal symptoms (GISs) can affect the performance of endurance athletes (EAs). This study aims to analyze the efficacy of carbohydrate (CHO), gluten-free, and low-mono-saccharide and polyol (FODMAP) diets in preventing GISs in adult EAs of both sexes. Methods: A systematic search was conducted prior to 30 June 2024 in accordance with the PRISMA statement. We searched for original studies from the last eight years, in English or Spanish, that looked at the effect of CHO, gluten-free, or FODMAP diets on the GISs of EAs. In PubMed, the MeSH (medical subject heading) categories were used. The search was repeated in EBSCO, Google Scholar, and Web of Science. The inclusion criteria were determined using the PICOS framework and the risk of bias in each paper was assessed using the PEDro scale quality criteria checklist (systematic review registration: INPLASY202490080). Results: Of 289 articles identified, only 3.5% met the eligibility criteria. All studies found that GISs are common in EAs. We found that 60% of the articles used an experimental method; moreover, based on 80% of the articles, following a bowel training diet, like CHO, reduced fiber and dairy products, or a low-FODMAP diet, has the potential to reduce gastrointestinal symptoms and improve the athletic performance of EA. Conclusions: We found that low-FODMAP diets, gut training with CHO intake, and decreased fiber and dairy intake may have favorable effects in preventing GISs. No studies support a gluten-free diet in reducing GISs in EAs.