bluuebunny/crossref_metadata_2025 huggingface dataset:
Kajian Literatur: Dampak Penerapan HACCP dan Sistem Mutu dalam Peningkatan Daya Saing pada Industri Pengolahan Ayam
Authors: Barki Khotimatul
Published: February 2024

Trade competition in the era of globalization is getting tougher, but food processing businesses from both agricultural and animal raw materials still have a good chance to survive. Products that have good quality and food safety can compete in domestic and international markets. To maintain food safety, business owners can implement quality assurance systems such as HACCP (Hazard Analysis Critical Control Point). HACCP needs to be implemented to further improve competitiveness and be able to run a business sustainably. The method used in this research is a systematic literature review. The result of this study is the application of HACCP is expected to improve quality and be able to increase sustainable competitiveness. Most of the chicken processing industry has implemented HACCP by the elements in the appropriate HACCP program. The application of HACCP ensures the quality of food quality and avoids health hazards in the final product. The implementation of HACCP requires continuous training and education on HACCP to ensure a more stable system is implemented and the need for the use of complete personal protective equipment (PPE) to maintain hygiene during the production process.
Roman Culture in the Ottonian World
Authors: Sarti Laury
Published: February 2024

Roman culture outlived its empire in the West. Any study aiming to assess its relevance in the medieval period must consider that related conceptions and cultural features may change over time. A significantly different definition may have applied to medieval periods compared to what we know from the time of the Roman Principate. This paper studies Roman culture in the Ottonian world by considering the significance and role attributed to the culture of the Roman past and the Byzantine present. The first section focuses on cultural elements related to politics and the exertion of power, the second deals with Roman culture in the material heritage, and the third discusses related non-material aspects. The study argues that although the Ottonians called themselves emperors of a Roman empire, elements that may have been conceived as Roman or were related to the Byzantine world were far from abundant and largely limited to the court. The Roman empire was part of their western heritage, while Roman and Byzantine models represented the sole known sets of adequate examples or sources of inspiration to represent western emperorship. These models had already been adopted and adapted by the Carolingians, who remained an important reference among their imperial successors. Still, while influences from Byzantine court culture were present at the Ottonian court, monastic and popular Byzantine culture was largely absent from the Ottonian world.

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In the Intimacy of the Dār al-Nisā’: The Residential Spaces of the Nasrid Sultanas at the Alhambra of Granada (Thirteenth-Fifteenth Centuries)
Authors: Boloix-Gallardo Barbara
Published: February 2024

Like many other dynasties in the History of Islam in the Middle Ages, the Banū Naṣr (1232-1492) created their own palaces as both their seat of power and royal residence at the Alhambra in Granada, the city chosen as the capital of their kingdom in 1238. During its long building process, which lasted from the thirteenth to the fifteenth centuries, the Alhambra became the dwelling of the different members of the Nasrid family, including not only the men but also the many women who made up the harem of this dynasty. This article aims to identify the residential spaces in the Alhambra where the daily life of the women of the Nasrid dynasty took place. To start with, we will reflect on the feminine nature of the Alhambra and the concept of the harem in this final phase of Andalusi history, also analysing the composition of the household of the Nasrid sultanas. We will then try to locate the specific areas of the Alhambra where the Dār al-nisā’ (or “house of women”) may have been located, gathering the sparse textual anecdotes reflecting the presence of women in different parts of this royal palace and resorting to personal interpretations due this scarcity of textual sources. For this, we will revisit the available Arabic sources and material culture from a gender perspective. With this study, we aim to turn the Alhambra into an academic field of debate and reflection on how the segregation of spaces according to gender category was organized in the context of both Islamic society and architecture.
Hanley, Two Houses, Two Kingdoms: A History of France and England, 1100–1300 (London: Yale University Press, 2022)
Authors: Storey Gabrielle
Published: February 2024

Review of: Catherine Hanley, Two Houses, Two Kingdoms: A History of France and England, 1100–1300 (London: Yale University Press, 2022)
Molecular Insights of Excessive Water Cut during Cyclic Gas Injection in Liquid-Rich Shale Reservoirs: Contributions of Hydrocarbon Condensation and Water Trapping
Authors: Chen Fangxuan , Wang Shihao , Nasrabadi Hadi
Published: February 2024

In a recent pilot test of cyclic gas injection (huff ‘n’ puff) in a Permian shale reservoir, excessive water product was observed, the reason for which remains unclear. In this work, we analyze the mechanisms of gas huff ‘n’ puff processes using molecular dynamics (MD) simulations and explain the reason for the high water-cut phenomenon. We aim to investigate the hydrocarbon-water-rock interactions during the gas injection as well as production within a shale rock in the pore scale. To mimic the heterogeneous pore structure of the shale rock, we have designed a pore system, including a bulk pore, a pore throat, and a dead-end pore. We simulate the distribution of different fluids during the initial equilibrium stage, the primary depletion stage, and the huff ’n’ puff stage. The results show that an excessive amount of water is trapped by the condensation mechanism in the larger pores during the primary depletion stage. The water is then recovered with the injection of working (lean) gases. Moreover, we have analyzed the effect of different injection gases (IGs) and found that carbon dioxide (CO2) yields a higher water cut compared with methane (C1). Moreover, our findings have revealed the trapping mechanisms of hydrocarbon-water mixtures in shale rocks and have highlighted the impacts of pore structures on the recovery of shale reservoirs. As such, we have provided a potential explanation of the observed phenomenon.
A Transfer Learning-Based Deep Learning Model for Automated Breast Cancer Identification in Mammograms
Authors: katiyar preeti
Published: February 2024

Breast cancer is a severe health issue that affects women worldwide, underscoring the need for reliable and effective screening techniques. The early detection, diagnosis, and treatment of breast cancer are made possible by computer-aided diagnostic (CAD) systems that rely on mammograms. This study introduces a unique deep learning model that uses transfer learning to identify and categorize breast cancer automatically. Several recent studies have shown that deep convolutional neural networks (DCNNs) can be used to diagnose breast cancer in mammograms with performances comparable to or even superior to those of human experts. To extract attributes from the Mammographic Image Analysis Society (MIAS) dataset, the proposed model uses pretrained convolutional neural network (CNN) architectures such as ResNet50 and Visual Geometry Group networks (VGG)-16. This novel deep learning model holds significant potential for enhancing the efficiency and accuracy of breast cancer detection and classification.
A preprint has previously been published [1]
Dietary intake data for patients with and without colorectal cancer: A logistic lasso regression analysis
Authors: He Lulu , Wu Yufei , Chen Yan , Zhao Chenyi , Li Wenjing , Lu Yujie , Guo Feng
Published: February 2024

Colorectal cancer is a global health challenge with high morbidity and mortality, but its causative factors remain unclear. In recent years, associations between various dietary patterns and colorectal cancer have been identified, but no studies have examined the association between macro- and micronutrient intake and colorectal cancer. This study analyzed the association between colorectal cancer and dietary intake using the logistic least absolute shrinkage and selection operator (LASSO). The data were derived from national data from the 1999–2010 National Health and Nutrition Examination Survey (NHANES) cycle. These data were further filtered to select those aged 50 years or older who self-reported having colorectal cancer (n = 168) and those who did not self-report having colorectal cancer (n = 649). LASSO regression is a new statistical shrinkage technique based on the R statistical software. In this study, LASSO was used to analyze the association between colorectal cancer and the variables from which the most relevant variables were selected. These variables included currently recognized risk factors for colorectal cancer and nutrients related to dietary intake. Age, sex, and race, which are recognised risk factors, still showed a significant association with colorectal cancer after LASSO regression shrinkage. For dietary intake of macro- and micronutrients, only thiamine (beta = 0.003) and zinc (beta = 0.0007) were positively associated with colorectal cancer. The results suggest that thiamine and zinc may be strongly associated with colorectal cancer. However, the results of the LASSO regression are based on statistically derived propensities and have not been validated by ex vivo experiments.
Characterization of Synoptic Systems Triggering Disasters in the State of Espírito Santo, Brazil
Authors: Silva Lindsay , Seluchi Marcelo Enrique
Published: February 2024

Despite the large amount of research about hydrometeorological disasters in southern Brazil, only a tiny part covers the State of Espírito Santo. The State is frequently affected by disasters of this nature. Therefore, this work aims to determine and characterize the types of synoptic systems that produce heavy rainfall and cause disasters in ES. Between 2013 and 2021, the S2ID database, synoptic charts from CPTEC, images from GOES 13 and 16 satellites, and precipitation data from INMET were used to select the dates and characterize the meteorological situation. Additionally, the ERA5 reanalysis was used for the construction of composite. It was found that disasters that affect ES occur mainly during the summer, which agrees with a thermodynamically more unstable atmosphere. The main systems identified can be described as follows: 1- Intense Frontal Systems related to blocking configuration in previous days that allow humid air advection from the Atlantic Ocean, through the presence of an anticyclone together with colder air at medium levels; 2- SACZ related to a warm anomalous anticyclone in the Atlantic and an intense low-pressure center located to the north, also showing a blocking pattern; 3- Troughs with slow displacement and low baroclinicity associated with high convective instability, acting as an extension of the Chaco Low and Thermo-Orographic Low, and 4- Cold core Cyclones with barotropic characteristics, located over ES, linked to warm blocking anticyclones, positioned south of about 40–45°S. In general, these patterns could be identified at least 48 hours in advance, observing disturbances at higher latitudes.
Analysis of Perfluoroalkyl Carboxylates and Perfluoroalkyl Sulfonates in Environmental Water by In-Port Arylation Gas Chromatography-Electron Impact Ionization-Mass Spectrometry
Authors: Tashiro Yutaka , Harada Kouji H. , Ikehara Takanori , Ito Takuma
Published: February 2024

Per- and polyfluoroalkyl substances (PFASs) are persistent environmental pollutants that have been used for various purposes. Although PFASs can pollute the environment in a variety of areas related to use, storage, and disposal of their products, there are insufficient data on the extent of PFASs pollution outside industrialized countries with their manufacturing facilities. Most of analyses depend on high-cost liquid chromatography-tandem mass spectrometry. In this study, we established a method to analyze anionic PFASs using gas chromatography-electron impact ionization-mass spectrometry with in-port arylation by diaryl iodonium. Extraction of PFASs from water samples was performed using solid phase extraction with reverse phase sorbent. Fourteen PFAS compounds could be detected from spiked water samples, and the detection limit ranged from 3.1 to 8.1 ng L− 1. Using this method, we analyzed groundwater samples from Okinawa Island, Japan, and detected PFASs up to a total concentration of 1.9 µg L− 1. This method uses relatively inexpensive analytical equipment, hence it can possibly enable surveys on PFAS contaminations in a wide range of regions and opportunities.
Mitochondria-synthesize melatonin to improve the quality and in vitro maturation as well as the embryonic development of porcine oocytes
Authors: Zhu Tianqi , Yan laiqing , ma Wenkui , xia Fan , Wang Likai , Ma Xiao , Li Guangdong , shen Zixia , wang Yiwei , Fu Yao , Ji Pengyun , wang Bingyuan , Zhang Lu , Liu Guoshi
Published: February 2024

Background
The in vitro maturation efficiency of porcine oocytes is relatively low and this limits the production of in vitro porcine embryos. Since melatonin is involved in the mammalian reproductive physiology, in this study, we have explored whether the endogenously produced melatonin can help the porcine oocyte in vitro maturation.
Results
We have found for the first time, that mitochondria are the major sites for melatonin biosynthesis in porcine oocytes. This mitochondrially originated melatonin reduces ROS production increases the activity of mitochondrial respiratory electron transport chain, mitochondrial biogenesis, and mitochondrial membrane potential and ATP production. Therefore, melatonin improves the quality of oocytes and their in vitro maturation. In contrast, the reduced melatonin level caused by siRNA to knockdown AANAT (siAANAT) is associated abnormal distribution of mitochondria and decreased the ATP level porcine oocytes and inhibits their in vitro maturation. These abnormalities can be rescued by melatonin supplementation. in addition, we found that siAANAT can switches the mitochondrial oxidative phosphorylation to glycolysis, a Warberg effect. This metabolic alteration can also be corrected by melatonin supplementation. All these activities of melatonin appear to be mediated by its membrane receptors since the non-selective melatonin receptor antagonist luzindole can blunt melatonin’s effects.
Conclusions
Taken together, the mitochondria of porcine oocyte can synthesize melatonin and improve the quality of oocyte maturation. The mitochondria of porcine oocyte can synthesize melatonin and improve the quality of oocyte maturation.These results provide an insight from novel aspect to study the oocyte maturation in the in vitro condition.
Neonatal meningitis induces ADHD-like behaviors via modulation of dopaminergic inputs by macrophages
Authors: Tanabe Shogo , Muramatsu Rieko
Published: February 2024

Neonatal meningitis often causes neurodevelopmental disorders, including attention deficit hyperactivity disorder (ADHD), in adulthood. However, the molecular mechanism by which neonatal meningitis induces brain dysconnectivity, leading to neurodevelopmental disorders, remains unclear. To investigate the pathological mechanisms of neonatal meningitis-induced neurodevelopmental disorders, we established mouse model of neonatal meningitis by injecting carrageenan, and found that mice with neonatal meningitis exhibited ADHD-like behaviors later in life and neural hyperactivation in the nucleus accumbens due to the increase in dopaminergic inputs induced by macrophages. RNA-sequencing revealed macrophages in carrageenan-treated mice express increased level of Semaphorin 4A (Sema4A), and treatment with a Sema4A neutralizing antibody prevented ADHD-like behaviors after carrageenan treatment. In addition, Sema4A concentration was increased in the cerebrospinal fluid of patients with meningitis, and Sema4A promoted dopaminergic synapse formation in human neurons. Collectively, these findings suggest macrophages increase dopaminergic inputs by producing Sema4A, which is a critical factor in the development of neonatal meningitis-induced ADHD-like behaviors.
Could long-term application of biomass improve the main driver’s soil attributes responsible for agricultural sustainability in the Amazonian periphery?
Authors: Moura Emanoel Gomes , Dadalto Danúbia Lemes , Coelho Marta Jordana Arruda , Carvalho Cristina Silva , Silva Lincon Matheus Araujo , Ramos ⁠Karen Alessandra Castro , Saraiva Wenner Vinicius Araújo , Rolim Anna Isabelle Lima , de Araújo Myrella Katlhen da Cunha , Vieira Kesia Rodrigues Silva , Gomes Gustavo Henrique Furtado , Barbosa Ester Paiva Alves , Barbosa Werlen Araújo , Aguiar Alana das Chagas Ferreira
Published: February 2024

The productivity and sustainability of many tropical agricultural systems must be increased to meet sustainability requirements to prevent deforestation of new non-agricultural areas. The availability and uptake efficiency of crop nutrients are fundamental considerations for any attempt to achieve this increase in productivity. We hypothesised that leguminous residues modify soil attributes through chemical and biological changes that affect plant growth and the long-term sustainability of agroecosystems in humid tropical regions. The present study aimed to determine the effects of leguminous biomass on the variations of soil organic matter fractions and soil basic cation contents and to evaluate the effects of these changes on the proportions of soil phosphorus (P) in different pools in a cropping system. A randomised block design with four replications and the following treatments was used for the modified alley cropping: Leucaena + Clitoria (L + C); Leucaena + Gliricidia (L + G); Leucaena + Acacia (L + A); Gliricidia + Acacia (G + A); Gliricidia + Clitoria (G + C), and no residues (NR). Our results confirmed the positive effects of the long-term application of leguminous biomass on those soil attributes that are the main drivers of the sustainability of tropical agroecosystems. However, such effects can differ depending on the biomass quality used. Indeed, the biomass of lower quality was more efficient in redirecting the fluxes of P towards the organic P pool, decreasing the less available Pi fractions. While the use of high-quality biomass from leucaena + gliricidia was superior to increase the stabilised carbon fraction in association with basic cations content. Ultimately, it can be concluded that to prevent cultivated land degradation and to avoid deforestation of new areas in the Amazonian periphery, production and application of high-quality biomass is an adequate strategy.
Functional partitioning of sentence processing and emotional prosody in the right perisylvian cortex after perinatal stroke
Authors: Martin Kelly C. , Seydell-Greenwald Anna , Turkeltaub Peter E. , Chambers Catherine E. , Gaillard William D. , Newport Elissa L.
Published: February 2024

In healthy adults different language abilities—sentence processing versus emotional prosody—are supported by the left (LH) versus the right hemisphere (RH), respectively. However, after LH stroke in infancy, RH regions support both abilities with normal outcomes. We investigated how these abilities co-exist in RH regions after LH perinatal stroke by evaluating the overlap in the activation between two fMRI tasks that probed auditory sentence processing and emotional prosody processing. We compared the overlap for these two functions in the RH of individuals with perinatal stroke with the symmetry of these functions in the LH and RH of their healthy siblings. We found less activation overlap in the RH of individuals with LH perinatal stroke than would be expected if both functions retained their typical spatial layout, suggesting that their spatial segregation may be an important feature of a functioning language system.
Multiomics approaches disclose very-early molecular and cellular switches during insect-venom allergen-specific immunotherapy
Authors: Hefeng Feng , Pogorelov Dimitrii , Bode Sebastian , He Xin , Ramiro-Garcia Javier , Hedin Fanny , Ammerlaan Wim , Konstantinou Maria , Capelle Christophe , Zeng Ni , Poli Aurélie , Domingues Olivia , Montamat Guillem , Hunewald Oliver , Cire Séverine , Baron Alexandre , Longworth Joseph , Neuberger-Castillo Lorie , Revets Dominique , Guyonnet Léa , Demczuk Agnieszka , Delhalle Sylvie , Zimmer Jacques , Benes Vladimir , Codreanu-Morel Françoise , Lehners-Weber Christiane , Weets Ilse , Alper Pinar , Brenner Dirk , Gutermuth Jan , Guérin Coralie , Morisset Martine , Hentges François , Schneider Reinhard , Shamji Mohamed , Betsou Fay , Wilmes Paul , Glaab Enrico , Goncalves Jorge , Cosma Antonio , Ollert Markus
Published: February 2024

Allergen-specific immunotherapy (AIT) induces immune tolerance, showing the highest success rate (>95%) for insect venom while a much lower chance for pollen allergy. However, the molecular switches leading to successful durable tolerance restoration remain elusive. Here we applied a multilayer-omics approach to reveal dynamic peripheral immune landscapes during AIT-initiation phase in venom allergy patients (VAP) versus pollen-allergic and healthy controls. Already at baseline, VAP exhibited altered abundances of several cell types, including antigen-presenting cells (APC) and hybrid types, especially pDC-mDC hybrids. At 8-24h following AIT launch in VAP, we identified a uniform AIT-elicited pulse of late-transitional/IL-10-producing B cells, IL-6 signaling within Th2 cells and non-inflammatory serum-IL-6 levels. Sequential induction of activation and survival protein markers also immediately occurred. A disequilibrium between serum IL-6 and APC in VAP baseline was restored at day seven following AIT launch. Our longitudinal analysis discovers molecular switches during initiation-phase insect-venom AIT that secure long-term outcomes.
Dipyridamole Reduces Lung Injury in a Rat Lung Ischemia and Reperfusion Model
Authors: Özgür Yücel , Altun Şenel , Işık Reyhan , Kılıçkaya Refika , Özcan Burcu , Şahin Nurettin , Köksal Bülent
Published: February 2024

Background: In this study, the effect of Dipyridamole (DIP) on lung tissue in lung ischemia/reperfusion (I/R) injury in rats was investigated.
Methods: A total of 24 Wistar rats were divided into four equal groups with six rats in each group: Group Sham, Group I/R (ischemia/reperfusion), and Group I/R+ 10mg/kg DIP, Group I/R+ 100mg/kg DIP. 45 minutes of ischemia and 120 minutes of reperfusion were applied.
Results: TNF-α value was decreased in Groups 10mg/kg DIP and I/R+ 100mg/kg DIP compared to Group I/R. TNF-α value value was lower in Groups 10mg/kg DIP and 100mg/kg DIP. The difference in TNF-α value was statistically significant between the groups (p=0.002). Statistically significant results were observed between Group I/R-DIP 100 mg/kg and Group I/R for TNF-α. (p=0.038).
MDA value in the blood was higher in Group I/R than in Group Sham. MDA value was decreased in Groups 10mg/kg DIP and 100mg/kg DIP compared to Group I/R. However, the difference in MDA value was not statistically significant between the groups (p=0.071). Catalase level in the blood was higher in Group I/R than Group Sham.). The difference in CAT activity was statistically significant between the groups (p=0.025). Statistically significant results were observed between Group I/R-DIP 100 and Group I/R for CAT. (p=0.047).
According to histopathological examination, lung tissue neutrophil infiltration, pulmonary edema, interstitial edema, alveolar hemorrhage and total score levels were significantly higher in Group I/R than Groups I/R-10mg/kg DIP and I/R-100mg/kg DIP(p=0.02, p=0.03, p=0.01, respectively, p=0.03 and p=0.01). Lung tissue pulmonary edema, interstitial edema, neutrophil infiltration, alveolar hemorrhage and total score levels were significantly lower in Group 10mg/kg DIP compared to Group I/R (p=0.03, p=0.01, p=0.02, p=0.03 and p=0.01, respectively). ). Lung tissue pulmonary edema, interstitial edema, neutrophil infiltration, alveolar hemorrhage and total score levels were significantly lower in Group I/R-100mg/kg DIP compared to Group I/R (p=0.009, p=0.002, p=0.001, p=0.002 and p=0.002, respectively). ).
Conclusion: Lung tissue may be affected by ischemia/reperfusion injury, and this damage can be reversed with the use of dipyridamole.
Astrocyte morphogenesis requires self-recognition
Authors: Zipursky S. , Lee John , Sergeeva Alina , Ahlsen Goran , Mannepalli Seetha , Bahna Fabiana , Goodman Kerry , Khakh Baljit , Weiner Joshua , Shapiro Lawrence , Honig Barry
Published: February 2024

Self-recognition is a fundamental cellular process across evolution and forms the basis of neuronal self-avoidance1-4. Clustered protocadherins (Pcdh), comprising a large family of isoform-specific homophilic recognition molecules, play a pivotal role in neuronal self-avoidance required for mammalian brain development5-7. The probabilistic expression of different Pcdh isoforms confers unique identities upon neurons and forms the basis for neuronal processes to discriminate between self and non-self5,6,8. Whether this self-recognition mechanism exists in astrocytes, the other predominant cell type of the brain, remains unknown. Here, we report that a specific isoform in the Pcdhγ cluster, γC3, is highly enriched in human and murine astrocytes. Through genetic manipulation, we demonstrate that γC3 acts autonomously to regulate astrocyte morphogenesis in the mouse visual cortex. To determine if γC3 proteins act by promoting recognition between processes of the same astrocyte, we generated pairs of γC3 chimeric proteins capable of heterophilic binding to each other, but incapable of homophilic binding. Co-expressing complementary heterophilic binding isoform pairs in the same γC3 null astrocyte restored normal morphology. By contrast, chimeric γC3 proteins individually expressed in single γC3 null mutant astrocytes did not. These data establish that self-recognition is essential for astrocyte development in the mammalian brain and that, by contrast to neuronal self-recognition, a single Pcdh isoform is both necessary and sufficient for this process.
Transparent and High-porosity Aluminum Alkoxide Network-forming Glasses
Authors: Zhao Yingbo , Zhang Zihui
Published: February 2024

Metal-organic network-forming glasses are an emerging type of material capable of combining the modular design and high porosity of metal-organic frameworks and the high processability and optical transparency of glasses. However, a generalizable strategy for achieving both high porosity and high glass forming ability in modularly designed metal-organic networks has yet to be developed. Herein, we developed a new series of metal-organic network-forming glasses, aluminum alkoxide glasses, by linking aluminum-oxo clusters with alcohol linkers in the presence of a modulator template. These glasses exhibit well-defined glass transitions and high surface areas up to 500 m2/g, making them one of the most porous glassy materials. The aluminum alkoxide glasses also have optical transparency and fluorescent properties, and their structures were elucidated by pair-distribution functions and compositional analysis. A systematic glass transition study suggested that progressive increase in network connectivity during the evaporation of a coordinatively competitive solvent is key to the bottom-up glass synthesis. Aluminum alkoxide glass can also encapsulate crystalline MOFs to yield composite materials with higher porosities. These findings could significantly expand the library of microporous metal-organic network-forming glasses and enable their future applications.
Study on hydraulic fracture propagation of strong heterogeneous shale based on stress-seepage damage coupling model
Authors: Liu Wei , Xiao Lei , Ke Yunbin , Dong Kangxing
Published: February 2024

The developed laminar structure of shale makes it have obvious anisotropic characteristics, and the anisotropic characteristics are one of the important factors leading to the strong and weak heterogeneous properties of shale, and there is a common phenomenon that the propagation of hydraulic fracture heights is limited in the hydraulic fracturing process of strong heterogeneous shale reservoirs. In order to clarify the reasons for the limited height propagation of hydraulic fractures in strongly heterogeneous shale reservoirs, numerical simulation methods were used to study the propagation patterns of hydraulic fractures in shale reservoirs under different anisotropy, flow rate, viscosity, and stress differences. The results show that as the anisotropy value increases and the heterogeneity becomes stronger, the more difficult it is for hydraulic fractures to expand along the fracture height direction, and the larger the fracture width is at the fracture opening. For the strong heterogeneous shale reservoir, the hydraulic fracture height can be increased within a certain range by increasing the flow rate, increasing the viscosity and increasing the stress difference. When the upper limit value is exceeded, the hydraulic fracture height of the strong heterogeneous shale reservoir gradually becomes stable, and the increasing trend is no longer obvious. The strong or weak heterogeneity caused by the inherent anisotropic characteristics of shale reservoirs will affect the development and transformation effectiveness of shale reservoirs. The research in this article will provide some reference and guidance for the efficient development of shale reservoirs.
Hallmarks of a Genomically Distinct Subclass of Head and Neck Cancer
Authors: Muijlwijk Tara , Nauta Irene , Lee Anabel van der , Grünewald Kari , Brink Arjen , Ganzevles Sonja , de Jong Robert Baatenburg , Atanesyan Lilit , s.savola@mrcholland.com Suvi , de Wiel Mark van , Peferoen Laura , Bloemena Elisabeth , de Ven Rieneke van , Leemans C. , Poell Jos , Brakenhoff Ruud
Published: February 2024

Cancer is caused by an accumulation of somatic mutations and copy number alterations (CNAs). Besides mutations, these copy number changes are key characteristics of cancer development, but nonetheless some tumors show hardly any CNAs, a remarkable phenomenon in oncogenesis. Head and neck squamous cell carcinomas (HNSCCs) arise by either exposure to carcinogens, or infection with the human papillomavirus (HPV). HPV-negative HNSCCs are generally characterized by many CNAs and frequent mutations in CDKN2A, TP53, FAT1 and NOTCH1. Here we present the hallmarks of the distinct subgroup of HPV-negative HNSCC with no or few CNAs (CNA-quiet) by genetic profiling of 802 oral squamous cell carcinomas (OCSCCs). In total, 73 OCSCC (9.1%) were classified as CNA-quiet and 729 as CNA-other. The CNA-quiet group was characterized by wild-type TP53, frequent CASP8 and HRAS mutations, and a less immunosuppressed tumor immune microenvironment with lower density of regulatory T cells. Patients with CNA-quiet OCSCC were older, more often women, less frequently current smokers and had a better 5-year overall survival compared to CNA-other OCSCC. This study demonstrates that CNA-quiet OCSCC should be considered as a distinct, clinically relevant subclass. Given the clinical characteristics, the patient group with these tumors will rapidly increase in the aging population.
Response surface approach of Propylene Glycol and Water (75:25)/Graphene+MWCNT nanofluid viscosity
Authors: Surakasi Raviteja , Manyala Gangadhar Rao , Ch Polayya
Published: February 2024

Response surface methodology (RSM) was used to study a Propylene Glycol-water nanofluid. The nano powder content was varied to make three PG + W (75:25) samples. Graphene and MWCNT nano particles were blended in base fluids at 0.25% and 0.5% using four-ball milling. Temperature decreases viscosity, and the combination PG + W (75:25) + 0.5% G + MWCNT has the lowest viscosity at 0.32 m2/s. The laboratory parameters in this study include a 40–120 ◦C temperature range and a 0 to 0.5% weight percentage. Finding the best prediction model and solution is the goal. The statistical-mathematical investigation of modelers' performance, contrast, and motives and the inconsistency between laboratory behaviour and real-world applications prompted this study. Some tested models are 2FI, quadratic, cubic, and quartic. Analysis of these modelling functions' statistical properties is a novel contribution to the area. Statistical research shows that the cubic model depicts nanofluids twice as accurately as other models. The R2 coefficient, C.V%, and P-value are used to evaluate models. The cubic model indices are 0.9936, 3.54%, and 0.0001. At 109.661 C, nanofluids should have 0.401 m2/sec viscosity and 0.278 weight percentage.
Associations of Helicobacter Pylori with Anemia: A Bidirectional Two-Sample Mendelian Randomization Study
Authors: Xia Chuqi , Lei Wanyang , Yang Yongyu , Sun Guowu , Zhang Yang , Li Xinyue , Xu Ning , Liang Daoming
Published: February 2024

Background
Observational studies have reported a correlation between Helicobacter pylori infection and an increased incidence of anemia, iron deficiency anemia (IDA), and vitamin B12 deficiency anemia. However, the fundamental cause remains elusive. This study aimed to ascertain the causal relationship between H. pylori infection and anemia.
Method
Using data from genome-wide association studies (GWAS), we executed a bidirectional Mendelian randomization (MR) study utilizing two-sample GWAS. The principal analytical method was the inverse variance weighted (IVW) method. We employed a conservative Bonferroni-corrected threshold (p < 2.38 × 10− 3). Sensitivity analyses were implemented with Cochran’s Q test and MR-Egger regression.
Results
Genetically predicted H. pylori infection, anti-H. pylori CagA, VacA, and IgG seropositivity was not causally associated with anemia, IDA, or vitamin B12 deficiency anemia (p > 2.38 × 10− 3). Furthermore, reverse MR analysis did not reveal evidence for a causal effect of anemia on H. pylori infection (p > 0.05).
Conclusions
Utilizing genetic data, MR analysis demonstrated the absence of evidence for a causal association between the seroprevalence of H. pylori infection and anemia. Further research is needed to elucidate the relationship between H. pylori infection and anemia.
Streamline topologies and bifurcation analysis for electroosmosis peristaltic flow of FENE-P fluid through an asymmetric channel
Authors: Ullah Kaleem , Shahzad Hasan , Algarni Salem , Alqahtani Talal , Irshad Kashif
Published: February 2024

The bifurcation of stagnation points and streamlines topologies of various flow phenomena for the transportation of FENE-P fluid due to electro-osmotic peristaltic activity have been investigated via dynamical system approach. The study focuses on the flow within an asymmetric channel, using well-known approximations that assume long wavelength and an extremely low Reynolds number. Autonomous differential equations have been established to point out the stagnation points. Three various flow distributions: backward, trapping and augmented flow are cropped up in the flow. The transition of these phenomena correspond two critical (bifurcation) conditions. Variations of the involved parameters have a notable impact on the streamline topologies in these flow distributions, as well as on the bifurcations of stagnation points. The trapping region is found to expand by enlarging the electric potential on the channel walls. However, increments in the Helmholtz-Smoluchowski velocity and electro-osmotic parameter reduces the trapping phenomenon in the flow. The findings acquired undergo examination through global bifurcation diagrams. This analysis could form the basis for constructing a mathematical framework. Such a framework would be useful in investigating the quantitative influences of the parameters involved in the diverse flow phenomena.
Real-World Effectiveness of First-Line Azacitidine or Decitabine with or without Venetoclax in AML Patients Unfit for Intensive Therapy
Authors: Acker Fabian , Chromik Jörg , Tiedjen Emily , Wolf Sebastian , Vischedyk Jonas B. , Makowka Philipp , Enßle Julius C. , Kouidri Khouloud , Sebastian Martin , Steffen Björn , Oellerich Thomas , Serve Hubert , Neubauer Andreas , Schäfer Jonas A. , Bittenbring Jörg T.
Published: February 2024

Standard frontline treatment in patients with acute myeloid leukemia (AML) unfit for intensive therapy is the combination of a hypomethylating agent (HMA) with venetoclax (VEN). However, retrospective data confirming the benefits of this regimen outside of clinical trials are sparse and have shown conflicting results. Thus, we performed a multicenter retrospective analysis of outcomes with HMA-VEN compared to HMA alone in patients with newly diagnosed AML unfit for intensive treatment. A total of 213 patients were identified from 3 German tertiary care centers. Of those, 125 were treated with HMA-VEN and 88 with HMA alone. Median overall survival (OS) in the HMA-VEN cohort was 7.9 months (95% confidence interval [CI], 5.1–14.7) compared to 4.9 months (3.1–7.1) with HMA alone. After 1 year, 42% (95% CI, 33–54) and 19% (12–30) of patients were alive, respectively. The hazard ratio (HR) for death was 0.64 (95% CI, 0.46–0.88; p = 0.006). After adjusting for age, NCCN cytogenetic risk, NPM1, RUNX1, and TP53 status, ECOG performance status, baseline leukocytes, and type of HMA, treatment with HMA-VEN remained significantly associated with a prolonged survival (HR, 0.48; 95% CI, 0.29–0.77). Accordingly, time to next treatment (TTNT) was longer with HMA-VEN with a HR of 0.63 (95% CI, 0.47–0.85). Patients who achieved recovery of peripheral blood counts had a favorable prognosis (HR for death, 0.52; 95% CI, 0.33–0.84). These data align with findings from the pivotal VIALE-A trial and support the use of HMA-VEN in patients unfit for intensive therapy.
Serum cytokines and creatinine/cystatin C ratio as prognostic biomarkers in advanced cancer patients treated with anti-PD-1/PD-L1 therapy
Authors: Jin Shan-xiu , Liu Bo-Na , Ji juan , Wu Jing-ran , Li Bao-lei , Gao Xiao-li , Li Na , Zheng Zhen-dong , Du Cheng
Published: February 2024

Objective
Immune checkpoint inhibitors (ICIs), specifically targeting the programmed cell death protein-1 or its ligand (PD-1/PDL-1), have been extensively used in the treatment of a spectrum of malignancies, although the predictive biomarkers remain to be elucidated. This study aims to investigate the association between baseline circulating levels of cytokines and the creatinine/cystatin C ratio (CCR) with the treatment outcomes of ICIs in patients with advanced cancer.
Methods
The pre-treatment circulating levels of 10 cytokines (PD-L1, CTLA4, CXCL10, LAG3, HGF, CCL2, MIG, GRANB, IL-18, and IL-6) were measured via automated capillary-based immunoassay platform in the serum of 65 advanced cancer patients treated with anti-PD-1/PD-L1-based systemic therapy and 10 healthy volunteers. The levels of cytokines and CCR were quantified and categorized into high and low groups based on the median value. The associations of serum cytokines and CCR with response to treatment, survival, and immune-related adverse events were assessed.
Results
Elevated circulating levels of 6 cytokines (PD-L1, CXCL10, HGF, CCL2, MIG, and IL-6) were observed in cancer patients compared with that in healthy volunteers. In the cancer cohort (N = 65), low circulating HGF (P = 0.023, P = 0.029), low IL-6 (P = 0.002, P < 0.001), and high CCR (P = 0.031, P = 0.008) were associated with significantly improved progression-free survival (PFS) and overall survival (OS). Multi-variable COX analyses adjusted for clinicopathological factors revealed that low HGF, low IL-6, and high CCR were independent favorable prognostic factors for PFS (P = 0.028, P = 0.010, and P = 0.015, respectively) and OS (P = 0.043, P = 0.003, and P = 0.026, respectively). Grade 2 irAEs occurred more frequently in patients with low levels of circulating CCL2 and LAG3.
Conclusions
Pre-treatment circulating levels of serum IL-6, HGF, and CCR may serve as independent predictive and prognostic biomarkers in advanced cancer patients treated with ICIs-based systemic therapy. These findings might help to identify potential patients who would benefit from these therapies.
Characterization of antibiotic resistomes and pathobiomes of soil antibiotic-degrading microbial by stable isotope probing and metagenomics
Authors: Wang Fang , Mei Zhi , He Chao , Fu Yuhao , Virta Marko , Liao Maoyuan , Hashsham Syed , Jiang Xin , Jia Zhongjun , Zhang Tong , Tiedje James
Published: February 2024

The presence and spread of antimicrobial resistance genes (ARGs) in soil represent a significant threat to human health. However, the ARGs harbored by antibiotic-degrading microorganisms in soil are inadequately characterized. Employing diverse molecular techniques, we investigate the dynamics of ARGs, mobile genetic elements, and virulence factors in soils contaminated with the antibiotic 13C-sulfadiazine (SDZ), enabling the identification of active SDZ-degrading bacteria. We find that the addition of biochar-based biofilm to various soil types reduces the abundance of several ARGs and virulence factors associated with active SDZ-degrading bacteria. Furthermore, our results reveal a marked increase in the relative abundance of specific genes within associated microorganisms during SDZ metabolism in the soil. This study elucidates the distribution and composition of antimicrobial resistance in active SDZ-degrading bacteria across diverse soils, suggesting that the utilization of biochar-based biofilm represents an effective strategy for mitigating antimicrobial resistance in soil within the One Health framework.
Role of HMGB1 in posttraumatic epilepsy and cognitive decline among traumatic brain injury patients: A prospective longitudinal study in Kuala Lumpur
Authors: Ngadimon Irma Wati , Mohan Devi , Shaikh Mohd. Farooq , Khoo Ching Soong , Tan Hui Jan , Lee Yu Mey , Chamhuri Nor Syazwani , Fadzil Farizal , Zolkafli Nursyazwana , Arulsamy Alina , Thanabalan Jegan , Aledo-Serrano Angel , Cheong Wing Loong
Published: February 2024

Background Posttraumatic epilepsy (PTE) and cognitive impairment are severe complications following traumatic brain injury (TBI). Neuroinflammation likely contributes, but the role of specific inflammatory mediators requires clarification. High mobility group box 1 (HMGB1) is an inflammatory cytokine released after brain injury that may be involved. This prospective longitudinal study investigated whether serum HMGB1 levels are associated with PTE development and cognitive decline over 12 months post-TBI.Methods Serum samples were collected from 41 TBI patients at baseline, 6, and 12 months following TBI. HMGB1 was quantified by ELISA alongside interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α). Cognitive assessment using validated neuropsychological assessments were performed at 6 and 12 months. The occurrence of PTE was also tracked.Results HMGB1 remained elevated at 12 months post-TBI only in the subgroup (n = 6) that developed PTE (p = 0.026). Higher HMGB1 levels at 12 months correlated with a greater decline in Addenbrooke's Cognitive Examination scores (p < 0.05). Reductions in HMGB1 (p < 0.05), IL-1β (p < 0.05) and TNF-α (p < 0.001) levels from 6 to 12 months correlated with improvements in cognitive scores. Multivariate regression analysis confirmed that HMGB1 level changes was independently associated with cognitive trajectory post-TBI (p = 0.003).Conclusions Persistently elevated HMGB1 post-TBI associates specifically with epileptogenesis and exacerbates cognitive dysfunction. HMGB1 links acute neuroinflammatory responses to chronic neurological outcomes. Longitudinal tracking supports HMGB1-directed interventions as a strategy for improving prognosis after TBI. Additional multicenter studies can clarify HMGB1's utility as a prognostic biomarker.
Citation metrics and quality of the top cited systematic reviews in dentistry: a meta-research
Authors: Pereira-Cenci Tatiana , Cenci Jaisson , Franco Marina Christ , Daneris Ândrea Pires , Cenci Maximiliano Sérgio , Montagner Anelise Fernandes
Published: February 2024

Objective: This study evaluated the methodological quality and risk of bias of the most cited systematic reviews in dentistry.
Methods: An electronic search was performed in Scopus database to select the 50 most cited systematic reviews, including any type of study design as primary studies, with outcomes in dental sciences. Two reviewers independently selected the studies and extracted the data items. Reporting of general components was assessed according to the PRISMA Statement. The methodological quality was assessed using the AMSTAR-2 tool checklist. The risk of bias was assessed using the ROBIS tool. The correlation between the citation and the AMSTAR-2 and ROBIS scores was also investigated.
Results: The 50 most cited systematic reviews in dentistry varied from 252 to 980 citations and were published between 2002 and 2015. The adherence to PRISMA items varied, presenting low adherence rates for some items. Most reviews presented critically low (n=43; 86%) methodological quality, while 3 studies showed low quality (6%), 3 presented moderate quality (6%), and only 1 study was of high quality (2%). Risk of bias assessment showed a high risk of bias mainly for domains 1 (n=32, 64%), 2 (n=24 studies, 48%), and 4 (n= 25, 50%). Coefficients between the number of citations with AMSTAR-2 and ROBIS scores showed weak correlation (rho < 0.28).
Conclusions: The most cited systematic reviews in dentistry present critically low methodological quality and a high risk of bias for several domains. Reporting guidelines adherence is also questionable regarding the analysis of the available data.
Clinical Significance: A poor-quality systematic review can provide misleading conclusions that might affect decisions. The readers of systematic reviews should be aware about those possible falls.
Evaluation of Errors and Uncertainties in Debris Flow Modeling with RAMMS
Authors: Majid GALOIE , MOTAMEDI Artemis
Published: February 2024

The Rapid Mass Movement Simulation (RAMMS) which is one of the most commonly used tools for debris flow modeling, especially in Europe, requires input data such as Digital Elevation Model (DEM), the Voellmy-fluid friction coefficients, etc. The main aim of this study is to evaluate the uncertainties in RAMMS outputs using various input data. For this purpose, a real debris flow event was considered whose observed data were available. After the calibration of the model, various input data were used to assess the deviation of the outputs from the corresponding calibrated one. Different scenarios were defined, which included different DEM cell sizes (1, 2, 3, 4, 5, 10, 15, and 20 meters), different values of friction coefficient, different directions of inflow, and active or inactive consideration of terrain curvature. The final results showed that only by increasing the DEM cell size, holding all other parameters constant, the accuracy of the model outputs (e. g. overall inundation area) reduced intensively which was up to 724% for DEM-20 m in comparison to DEM-1 m. Also, the results showed that the impact of the Dry-Coulomb type friction coefficient (µ) was much greater than the viscous-turbulent friction coefficient (ξ) on the results. In addition, although the curvature of the debris flow path in this study was relatively sound, the effect of the terrain curvature activation on the results was not significant. Furthermore, changing in inflow direction to up to ± 50 degrees did not cause a significant change in the overall results.