bluuebunny/crossref_metadata_2025 huggingface dataset:
Pasture Access Effects on the Welfare of Dairy Cows Housed in Free-Stall Barns
Authors: Blaga Petrean Anamaria , Daina Sorana , Borzan Mihai , Macri Adrian M. , Bogdan Liviu , Lazar Eva A. , Popescu Silvana
Published: January 2024

Despite considerable research regarding the benefits of natural living conditions on several aspects of the health and well-being of dairy cows, the effects of pasture access on their overall welfare are less studied. In this comparative study, the Welfare Quality® protocol was applied in 22 zero-grazing and 17 grazing access farms with an ulterior statistical exploration of the differences found. Moreover, correlations were calculated between pasture access and animal-based welfare measures. Aside from the multiple benefits of pasturing identified within the welfare measures, criteria, and principles, in the overall classification, the farms with permanent confinement ranked lower than the grazing farms. Although both systems used free-stall barns, allowing the cows’ movement, the grazing animals showed improved overall welfare. Yet, the origin-related adaptation of the animals could play a role. The authors recommend research-based tailoring whenever these conditions are intended to be transposed in technology, especially in intensive systems.
The Influence of Surrounding Arable Fields on the Species Diversity and Composition of Isolated Mountain Mesic Grassland Patches
Authors: Pruchniewicz Daniel , Żołnierz Ludwik , Dradrach Agnieszka
Published: January 2024

This study aimed to determine the impact of arable fields on the diversity and species composition of isolated meadow patches of the order Arrhenatheretalia. The study was conducted in the Sowie Mountains and the adjacent hills (SW Poland). The species composition of the vegetation was analyzed along transects designated from the edge up to 33 m into the meadow patches. The species composition varied significantly in zones directly affected by arable fields, proving their strong negative impact on the vegetation of the mesic grasslands examined. The highest values of the diversity index were recorded 9 m away from the edge, while the lowest values were found in the direct influence zone of arable fields—2 m from the edge. In the case of apophytes, synanthropic species of local origin, the lowest values were recorded within 1 m and the highest values were recorded within 9 m. A significant change in the percentage of graminoids and dicotyledonous herbs was found between 9 m and 33 m from the edge. The study found no significant differences in habitat parameters measured with ecological indicators. Species of the Artemisietea vulgaris class effectively penetrate deep into grassland patches and can visibly degrade their vegetation.
Soil Phosphorus Forms in Saline Soil after the Application of Biomass Materials
Authors: Guan Xuewei , Chen Jinlin , Liu Guangming , Wang Xiuping
Published: January 2024

Although the application of biological materials has shown potential in improving the environment of salinized soil, the impact on the transformation of soil phosphorus forms in salinized soil, especially when combined with B. mucilaginosus (BM), has rarely been studied. Therefore, this study investigated soil’s properties and phosphorus forms, as well as their relationship, in salinized soil after the application of different biological materials, including rice straw (RS), biochar (B), acidified biochar (AB), BM, RS+BM, B+BM, and AB+BM. A sequential extraction procedure was employed to determine the soil organic/inorganic phosphorus forms (Po/Pi). In our study, the contents of the soil’s resin-P, NaHCO3-Pi, NaOH-Pi, and HCl-P increased by 50–341.66%, 4.08–184.63%, 37.45–163.45%, and 10.19–38.29%, respectively, with the application of the biological materials compared to with conventional fertilization (CK1). However, the contents of the soil’s NaHCO3-Po, NaOH-Po, and residual-P significantly decreased in comparison to with CK1, particularly in the soils that received a combined application with biological materials and BM. Furthermore, the results from the statistical analysis revealed that the application of biological materials could increase the level of soil stable phosphorus, facilitate the transformation from soil stable phosphorus and soil unstable Po to soil unstable Pi, and enhance the effectiveness of soil phosphorus, especially when combined with BM, particularly in soil with AB+BM.
Fostering Agricultural Transformation through AI: An Open-Source AI Architecture Exploiting the MLOps Paradigm
Authors: Cob-Parro Antonio Carlos , Lalangui Yerhard , Lazcano Raquel
Published: January 2024

As the global population is expected to reach 10 billion by 2050, the agricultural sector faces the challenge of achieving an increase of 60% in food production without using much more land. This paper explores the potential of Artificial Intelligence (AI) to bridge this “land gap” and mitigate the environmental implications of agricultural land use. Typically, the problem with using AI in such agricultural sectors is the need for more specific infrastructure to enable developers to design AI and ML engineers to deploy these AIs. It is, therefore, essential to develop dedicated infrastructures to apply AI models that optimize resource extraction in the agricultural sector. This article presents an infrastructure for the execution and development of AI-based models using open-source technology, and this infrastructure has been optimized and tuned for agricultural environments. By embracing the MLOps culture, the automation of AI model development processes is promoted, ensuring efficient workflows, fostering collaboration among multidisciplinary teams, and promoting the rapid deployment of AI-driven solutions adaptable to changing field conditions. The proposed architecture integrates state-of-the-art tools to cover the entire AI model lifecycle, enabling efficient workflows for data scientists and ML engineers. Considering the nature of the agricultural field, it also supports diverse IoT protocols, ensuring communication between sensors and AI models and running multiple AI models simultaneously, optimizing hardware resource utilization. Surveys specifically designed and conducted for this paper with professionals related to AI show promising results. These findings demonstrate that the proposed architecture helps close the gap between data scientists and ML engineers, easing the collaboration between them and simplifying their work through the whole AI model lifecycle.
The Effect of Using Elements of Sustainable Agrotechnology in Spring Wheat (Triticum aestivum L.) Monoculture
Authors: Kotwica Karol , Gałęzewski Lech , Kubiak Waldemar
Published: January 2024

In sustainable cultivation technologies, the method of managing crop residues and the microbiological activity of the soil, supported by the application of effective microorganisms, is of particular importance. Unfortunately, wheat monocultures are still common. Therefore, there is also a need to introduce elements of sustainable agrotechnics to such crops. The aim of the research was to compare the effect of 18 spring wheat (Triticum aestivum L.) cultivation technologies in a monoculture. Therefore, a four-year two-factor experiment was carried out with a spring wheat monoculture. Six ways managing the organic matter before sowing and tillage (first factor) and the application of microbiological preparations (second factor) were tested, leasing to a total of 18 experimental objects. The parameterized weed infestation, chlorophyll index, and leaf area index, elements of the yield structure, and spring wheat yield were determined through tillage technology. In most cases, the application of biopreparations was not found to have a significant impact on the tested features. The highest yields of spring wheat were obtained through the following technologies: application of EM or UGmax microbiological preparations on the shredded straw of the forecrop; mixing the forecrop with the soil using a grubber immediately after harvest; sowing the white mustard catch crop; winter plowing.
Improving the Key Enzyme Activity, Conversion Intensity, and Nitrogen Supply Capacity of Soil through Optimization of Long-Term Oilseed Flax Rotation Planting Patterns in Dry Areas of the Loess Plateau of China
Authors: Gao Yuhong , Zhang Yong , Wang Haidi , Wu Bing , Li Yue , Yan Bin , Wang Yifan , Lu Peina , Wang Ruijun , Wen Ming , Ma Xingkang , Xu Peng , Xue Wenfang , Chao Changyan , Wen Zedong
Published: January 2024

Various crop rotation patterns can result in differences in nutrient consumption and the accumulation of toxic substances in the soil, indirectly impacting the soil environment and its nutrient supply capacity. Implementing optimized crop planting practices is beneficial for maintaining the favorable physical and chemical properties of farmland soil in the arid area of northwestern China. This study aimed to establish a crop rotation pattern to improve key enzyme activities and soil nitrogen conversion efficiency, as well as understand the underlying mechanism for enhancing nitrogen supply capacity. A field experiment was conducted to study the effect of four flax planting patterns, which included 13 crop rotation patterns with different crop frequencies: 100% Flax (Cont F), 50% Flax (I) (WFPF, FPFW, PFWF, FWFP), 50% Flax (II) (FWPF, WPFF, PFFW, FFWP), 25% Flax (WPWF, PWFW, WFWP, FWPW), on the key enzyme activities and the rate of soil nitrogen conversion, as well as the nitrogen supply capacity. Here, F, P, and W represent oilseed flax, potato, and wheat, respectively. The results indicated that the wheat stubble significantly increased the intensity of soil ammonification and denitrification before planting. Additionally, the activity levels of soil nitrate reductase and nitrite reductase under wheat stubble were significantly increased by 66.67% to 104.55%, while soil urease activity significantly decreased by 27.27–133.33% under wheat stubble compared to other stubbles. After harvest, the activities of soil nitrate reductase and nitrite reductase under the wheat stubble decreased significantly, and the intensity of soil ammonification, nitrification, and denitrification reduced significantly by 7.83–27.72%. The WFWP and FWFP treatments led to a significant increase in soil nitrogen fixation intensity under various crop rotations after harvest and significantly increased the levels of inorganic nitrogen in the soil before the planting of the next crop. This study suggests that the long-term rotation planting patterns WFWP and FWFP can significantly enhance the key enzyme activities of soil nitrogen conversion and significantly improve soil nitrogen conversion before crop sowing. This may increase the rate of soil nitrogen transfer and raise the available nitrogen content of the soil. These findings are crucial for reducing soil nitrogen loss and improving soil nitrogen nutrient supply capacity in dry areas of the Loess Plateau of China.
Assessing the Impact of Genotype-by-Environment Interactions on Agronomic Traits in Elite Cowpea Lines across Agro-Ecologies in Nigeria
Authors: Popoola Bosede Olufunke , Ongom Patrick Obia , Mohammed Saba B. , Togola Abou , Ishaya Daniel Jockson , Bala Garba , Fatokun Christian , Boukar Ousmane
Published: January 2024

The yield of cowpea varieties is affected by environmental variability. Hence, candidate varieties must be tested for yield stability before release. This study assessed the impacts of genotypes, environments, and their interaction on the performance of elite cowpea lines for key adaptive, grain yield, and associated traits across different locations. A total of 42 elite genotypes were evaluated in five Nigerian environments, representing various savanna ecologies, during the 2021 growing season. The experimental design employed was an alpha lattice arrangement, with each genotype replicated three times. The results revealed significant differences among genotypes, environments, and genotype-by-environment interaction (G × E) for most traits, including days to maturity, 100-seed weight, and grain yield. The genotype and genotype-by-environment interaction (GGE) biplot showed G21 (IT14K-2111-2) and G25 (IT15K-2386-1) as the most stable genotypes across the five environments, G41 (IT11K-61-82) was best adapted to Ibadan and Shika, G5 (245-1) was best adapted to Bagauda and Gumel, and G30 (IT16K-2365-1) was best adapted to Bauchi. G21 (IT14K-2111-2) and G25 (IT15K-2386-1) could be recommended across the five test environments, whereas G41 (IT11K-61-82), G30 (IT16K-2365-1), and G5 (245-1) were specific to the adapted environments.
Quantitative Trait Loci Mapping for Adult-Plant Stripe Rust Resistance in Chinese Wheat Cultivar Weimai 8
Authors: Yan Xiaocui , Zhang Xiaoling , Kou Mengyun , Gebrewahid Takele Weldu , Xi Jiaxin , Li Zaifeng , Yao Zhanjun
Published: January 2024

Wheat stripe rust, triggered by Puccinia striiformis f. sp. tritici, is among the most widespread and damaging wheat (Triticum L.) diseases. The development of cultivars harboring adult plant resistance (APR) to stripe rust is a better approach to control the disease. The current study aimed to map APR to stripe rust via the QTL mapping of 165 F2–6 recombinant inbred lines (RILs), derivatives of Weimai 8/Zhengzhou 5389. The collection of phenotypic data for the stripe-rust resistance of both parents and all 165 RILs were conducted at Baoding, Hebei Province, during the 2016–2017 cropping seasons, and at Mianyang, Sichuan Province, during the 2017–2018 and 2018–2019 sowing seasons. The RIL populations and parents were also genotyped with 860 pairs of simple-sequence-repeat (SSR) primers to map APR QTLs to stripe-rust resistant. Moreover, a 55K SNP chip was used for small group bulk segregant analysis conducted to locate the genetic map location and concentration of the SNP markers on the wheat genome. Inclusive composite interval mapping (IciMapping 3.2) software identified four QTLs of stripe-rust resistance on chromosomes 1B, 2AS, 2BS, and 7DS, named QYr.wmy-1B, QYr.wmy-2AS, QYr.wmy-2BS, and QYr.wmy-7DS, which significantly explained 11.56–16.64%, 9.35–12.70%, 7.27–9.95%, and 11.49–15.07% of the phenotypic variation, respectively. All these QTLs were found from the resistant parent, Weimai 8. Meanwhile, the QTLs located on chromosomes 1B and 2AS were found close to Yr18 and Yr9, respectively. Furthermore, the results indicated that QYr.wmy-1B is possibly Yr9 and QYr.wmy-2AS is similar to Yr18 or might be a new QTL, whilst QYr.wmy-2BS and QYr.wmy-7DS were found to be different from previously reported stripe-rust-resistance QTLs and are possibly new QTLs. Overall, the QTLs and their closely associated molecular markers detected in this study could be a great source of input for marker-assisted selection to adult plant stripe-rust resistance in wheat-breeding programs.
Tillage with Crop Residue Returning Management Increases Soil Microbial Biomass Turnover in the Double-Cropping Rice Fields of Southern China
Authors: Tang Haiming , Li Chao , Shi Lihong , Wen Li , Li Weiyan , Cheng Kaikai , Xiao Xiaoping
Published: January 2024

The variety of soil microbial biomass carbon (SMBC), soil microbial biomass nitrogen (SMBN) content, and the flux turnover rate of SMBC and SMBN for 0–10 cm and 10–20 cm layers in a paddy field in southern China with different tillage practices were studied. The tillage experiment included conventional tillage and crop residue returning (CT), rotary tillage and crop residue returning (RT), no–tillage and crop residue returning (NT), and rotary tillage with all crop residues removed from the paddy field as a control (RTO). The result showed that the SMBC and SMBN contents at 0–10 cm and 10–20 cm layers in the paddy field with CT, RT, and NT treatments were significantly increased. This result indicates that the flux turnover rate of SMBC and SMBN for 0–10 cm and 10–20 cm layers in the paddy field with CT treatment were increased by 65.49%, 39.61%, and 114.91%, 119.35%, compared with the RTO treatment, respectively. SMBC and SMBN contents and the flux turnover rate of SMBC and SMBN for the 0–10 cm layer were higher than that of the 10–20 cm layer in paddy fields under the same tillage condition. Therefore, applying rotary tillage or conventional tillage and crop residue returning produced beneficial management for increasing soil microbial biomass content and its turnover under a double–cropping rice system in southern China.
Enhanced Cotton Yield and Fiber Quality by Optimizing Irrigation Amount and Frequency in Arid Areas of Northwest China
Authors: Jia Yaoyu , Yang Beifang , Han Yingchun , Wang Guoping , Su Tianle , Li Xiaofei , Lei Yaping , Zhi Xiaoyu , Xiong Shiwu , Xin Minghua , Li Yabing , Feng Lu
Published: January 2024

Optimizing irrigation strategies is crucial for sustaining cotton production in the face of growing water scarcity. The three-year experimental study (2020–2022) focused on the impact of varying irrigation amounts (320, 370, and 420 mm) and frequencies (4-, 8-, and 12-day intervals) on cotton growth, yield, yield components, and fiber quality in southern Xinjiang. Employing a completely randomized design with three replications, the results indicated higher irrigation amounts resulted in numerically 2.5–7.5% higher lint yields compared to those under medium and low irrigation amounts, notably significant in 2021. Boll density emerged as the primary yield component influencing yield loss due to irrigation amount, followed by seed cotton weight boll−1. Increased boll density was associated with a greater total number of fruiting sites. Additionally, the increased seed cotton weight boll−1 could be linked to an increased seed number boll−1 and a decreased boll fraction at the lower fruiting branches with lower seed cotton weight boll−1. Higher irrigation frequency improved lint yield by increasing boll density, though a significant effect was observed only in 2020. Increased irrigation amounts generally led to longer fiber lengths but lower micronaire values, while increased irrigation frequency resulted in longer, stronger fibers and reduced micronaire values. Furthermore, the highest yield stability was observed under the condition of high irrigation amount and high frequency. This study holds certain guiding significance for water resource management in cotton production in arid regions.
Environmental Impact of Various Rice Cultivation Methods in Northeast China through Life Cycle Assessment
Authors: Wang Yu , He Wenqing , Yan Changrong , Gao Haihe , Cui Jixiao , Liu Qin
Published: January 2024

Rice, a crucial staple in China, is cultivated through various techniques, including seedling transplanting, dry direct seeding, and film mulching. Despite its significance, rice production is a considerable environmental burden. Using a life cycle assessment (LCA) methodology, this study aimed to evaluate the environmental impacts of four rice cultivation methods (transplanting rice, dry direct-seeding rice, dry direct-seeding rice with polyethylene film (PE), and dry direct-seeding rice with biodegradable film) in Northeast China. The results indicate that the magnitude of environmental impacts among treatments was consistent across years. The potential values of all environmental impacts of the four different cultivation methods of rice in the 2021 field trial were smaller than the results of the same cultivation method of rice system in the 2022 field trial. Among the four rice cultivation methods, the consumption of energy showed inconsistency over the two years, with the highest energy consumption in the first year being for dry seeding with PE film and in the second year for dry seeding without film. Additionally, transplanting exhibited the highest impact on water resource consumption and climate change. Dry direct-seeding rice displayed the highest eutrophication and ecotoxicity. Dry direct-seeding rice with a biodegradable film had the least impact in terms of acidification. Moreover, dry direct-seeding rice with a biodegradable film minimized water consumption and greenhouse gas emissions without compromising yield.
Analysis and Prediction of Land Use/Land Cover Changes in Korgalzhyn District, Kazakhstan
Authors: Alipbeki Onggarbek , Alipbekova Chaimgul , Mussaif Gauhar , Grossul Pavel , Zhenshan Darima , Muzyka Olesya , Turekeldiyeva Rimma , Yelubayev Dastan , Rakhimov Daniyar , Kupidura Przemysław , Aliken Eerassyl
Published: January 2024

Changes occurring because of human activity in protected natural places require constant monitoring of land use (LU) structures. Therefore, Korgalzhyn District, which occupies part of the Korgalzhyn State Natural Reserve territory, is of considerable interest. The aim of these studies was to analyze changes in the composition of the land use/land cover (LULC) of Korgalzhyn District from 2010 to 2021 and predict LU transformation by 2030 and 2050. Landsat image classification was performed using Random Forest on the Google Earth Engine. The combined CA-ANN model was used to predict LULC changes by 2030 and 2050, and studies were carried out using the MOLUSCE plugin. The results of these studies showed that from 2010 to 2021, there was a steady increase in the share of ploughable land and an adequate reduction in grassland. It is established that, in 2030 and 2050, this trend will continue. At the same time, there will be no drastic changes in the composition of other land classes. The obtained results can be helpful for the development of land management plans and development policies for the Korgalzhyn District.
A Bilateral Acetabular Physeal Fracture Treated with External Fixation in an Immature Cat
Authors: Flores Jose Antonio , Rovesti Gian Luca , Rodriguez-Quiros Jesus
Published: January 2024

This study aimed to assess the outcome of a bilateral acetabular physeal fracture treated with external fixation in an immature cat, a surgical technique not usually employed in immature patients. The fixator took 40 days, and it was removed after radiographic bone healing was achieved. No significant complications related to the technique were identified, and the outcome was classified as good based on the functional assessment and pain scales employed. The use of external fixation for stabilising acetabular fractures in immature cats should be considered a viable technical option, especially for minimally invasive stabilisation.
Rye Bran as a Component in the Diets of Lactating Sows—Effects on Sow and Piglet Performance
Authors: Homann Christian , Wilke Volker , Eckey Isabell , Chuppava Bussarakam , Kaltschmitt Martin , Zimmermann Andreas , Visscher Christian
Published: January 2024

From a cost and sustainability perspective, the use of by-products such as rye bran in sow diets is of particular interest. Rye bran has valuable ingredients that have potential benefits for the gut health of sows. The aim of this study was to investigate the effects of including 15% rye bran in the sows’ feed on the performance of sows and piglets. The feeding started one week before the farrowing date and ended at weaning. Performance was evaluated by measuring sow (n = 175) and piglet body weight (n = 1372) and sows’ backfat thickness (n = 80). These data were additionally used to calculate the colostrum intake of the suckling piglets and the sows’ milk production. It was found that there were no differences in the performance parameters between the experimental and control groups. However, this study showed that the piglets with light birth weight (LBW (<1000 g)) and medium birth weight (MBW (1000–1500 g) consumed more colostrum when the sows were fed rye bran (LBW: C/R 203.0 ± 39.2 g/214.3 ± 35.9 g; MBW: 291.3 ± 39.0 g/298.5 ± 36.4 g). It can be concluded that including 15% rye bran in the feed of lactating sows has no obvious negative effects on the performance of sows and piglets. Further studies are needed to evaluate the possible positive effects of rye bran.
STC2 Inhibits Hepatic Lipid Synthesis and Correlates with Intramuscular Fatty Acid Composition, Body Weight and Carcass Traits in Chickens
Authors: Cao Yuzhu , Jia Qihui , Xing Yuxin , Ma Chenglin , Guan Hongbo , Tian Weihua , Kang Xiangtao , Tian Yadong , Liu Xiaojun , Li Hong
Published: January 2024

Stanniocalcin 2 (STC2) is a secreted glycoprotein involved in multiple biological processes. To systemically study the biological role of STC2 in chickens, phylogenetic tree analysis and conservation analysis were conducted. Association analysis between variations in the STC2 gene and the economic traits of Gushi-Anka F2 was conducted. The tissue expression patterns of STC2 expression in different chicken tissues and liver at different stages were detected. The biological role of STC2 in chicken liver was investigated through overexpression and interfering methods in the LMH cell line. Correlation analyses between STC2 expression and lipid components were conducted. (1) The phylogenetic tree displayed that chicken STC2 is most closely related with Japanese quail and most distantly related with Xenopus tropicalis. STC2 has the same identical conserved motifs as other species. (2) rs9949205 (T > C) found in STC2 intron was highly significantly correlated with chicken body weight at 0, 2, 4, 6, 8, 10 and 12 weeks (p < 0.01). Extremely significant correlations of rs9949205 with semi-evisceration weight (SEW), evisceration weight (EW), breast muscle weight (BMW), leg muscle weight (LMW), liver weight and abdominal fat weight (AFW) were revealed (p < 0.01). Significant associations between rs9949205 and abdominal fat percentage, liver weight rate, breast muscle weight rate and leg muscle weight rate were also found (p < 0.05). Individuals with TT or TC genotypes had significantly lower abdominal fat percentage and liver weight rate compared to those with the CC genotype, while their body weight and other carcass traits were higher. (3) STC2 showed a high expression level in chicken liver tissue, which significantly increased with the progression of age (p < 0.05). STC2 was observed to inhibit the content of lipid droplets, triglycerides (TG) and cholesterol (TC), as well the expression level of genes related to lipid metabolism in LMH cells. (4) Correlation analysis showed that the STC2 gene was significantly correlated with 176 lipids in the breast muscle (p < 0.05) and mainly enriched in omega-3 and omega-6 unsaturated fatty acids. In conclusion, the STC2 gene in chicken might potentially play a crucial role in chicken growth and development, as well as liver lipid metabolism and muscle lipid deposition. This study provides a scientific foundation for further investigation into the regulatory mechanism of the STC2 gene on lipid metabolism and deposition in chicken liver.
Alterations in Rumination, Eating, Drinking and Locomotion Behavior in Dairy Cows Affected by Subclinical Ketosis and Subclinical Acidosis
Authors: Antanaitis Ramūnas , Džermeikaitė Karina , Krištolaitytė Justina , Ribelytė Ieva , Bespalovaitė Agnė , Bulvičiūtė Deimantė , Rutkauskas Arūnas
Published: January 2024

This study delves into the effects of subclinical ketosis (SCK) and subclinical acidosis (SCA) on various parameters related to dairy cow rumination, eating, drinking and locomotion behavior. The research hypothesized that these subclinical metabolic disorders could affect behaviors such as rumination, feeding, and locomotion. A total of 320 dairy cows, with a focus on those in their second or subsequent lactation, producing an average of 12,000 kg/year milk in their previous lactation, were examined. These cows were classified into three groups: those with SCK, those with SCA, and healthy cows. The health status of the cows was determined based on the milk fat–protein ratio, blood beta-hydroxybutyrate, and the results of clinical examinations performed by a veterinarian. The data collected during the study included parameters from the RumiWatch sensors. The results revealed significant differences between the cows affected by SCK and the healthy cows, with reductions observed in the rumination time (17.47%) and various eating and chewing behaviors. These changes indicated that SCK had a substantial impact on the cows’ behavior. In the context of SCA, the study found significant reductions in Eating Time 2 (ET2) of 36.84% when compared to the healthy cows. Additionally, Eating Chews 2 (EC2) exhibited a significant reduction in the SCA group, with an average of 312.06 units (±17.93), compared to the healthy group’s average of 504.20 units (±18.87). These findings emphasize that SCA influences feeding behaviors and chewing activity, which can have implications for nutrient intake and overall cow health. The study also highlights the considerable impact of SCK on locomotion parameters, as the cows with SCK exhibited a 27.36% reduction in the walking time levels. These cows also displayed reductions in the Walking Time (WT), Other Activity Time (OAT), and Activity Change (AC). In conclusion, this research underscores the critical need for advanced strategies to prevent and manage subclinical metabolic disorders within the dairy farming industry. The study findings have far-reaching implications for enhancing the well-being and performance of dairy cattle. Effective management practices and detection methods are essential to mitigate the impact of SCK and SCA on dairy cow health and productivity, ultimately benefiting the dairy farming sector.
Status Quo of Feline Leukaemia Virus Infection in Turkish Cats and Their Antigenic Prevalence
Authors: Korkulu Emrah , Şenlik Elif İrem , Adıgüzel Ece , Artut Fatma Gökçe , Çetinaslan Hüseyin Doğukan , Erdem-Şahinkesen Eda , Oğuzoğlu Tuba Çiğdem
Published: January 2024

Feline leukaemia virus (FeLV) is a member of the Gammaretrovirus genus, which has two genotypes in cats: endogenous (replication-defective provirus) and exogenous (replication-competent). In this study, 550 cats were examined, and 112 of them (20.36%) were found to have the endogenous FeLV (enFeLV) genotype. EnFeLV-positive animals were also tested for additional viral infections, and 48 cats (42.85%) were discovered to be co-infected with other viruses. According to co-infection data, these cats were infected with feline coronavirus (FCoV) (27/112, 24.1%), feline panleukopenia virus (FPV) (14/112, 12.5%), feline immunodeficiency virus (FIV) (0/112, 0%), and domestic cat hepadnavirus (DCH) (13/112, 11.6%). Their age, sex, breed, clinical state, lifestyle (in/outdoor), and immunization data against FeLV were also evaluated. In line with our results, the prevalence of enFeLV and co-infection with other pathogens in cats admitted to the clinic for various reasons were discussed. The majority of positive animals in terms of FeLV (94/112, 83.93%) had clinical findings. We emphasized that the FeLV-positive situation of cats should be taken into consideration by veterinarians when planning treatment and vaccination programs. Additionally, in this study, we questioned the group in which our enFeLVs were phylogenetically located. Therefore, we performed a phylogenetic analysis based on a comparison with global FeLV sequences obtained from the GenBank database. The sequenced positive samples were in the AGTT subgroup within Group-II.
A New Benzo[6,7]oxepino[3,2-b] Pyridine Derivative Induces Apoptosis in Canine Mammary Cancer Cell Lines
Authors: Jianpraphat Natamon , Supsavhad Wachiraphan , Ngernmeesri Paiboon , Siripattarapravat Kannika , Soontararak Sirikul , Akrimajirachoote Nattaphong , Phaochoosak Napasorn , Jermnak Usuma
Published: January 2024

CMC is the most frequently diagnosed cancer and one of the leading causes of death in non-spayed female dogs. Exploring novel therapeutic agents is necessary to increase the survival rate of dogs with CMC. MPOBA is a BZOP derivative that has a significant anticancer effect in a human cell line. The main goal of this study was to investigate the anticancer properties of MPOBA against two CMC cell lines (REM134 and CMGT071020) using a 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, a wound healing assay, a transwell migration assay, an Annexin V-FITC apoptosis assay with a flow cytometry analysis, a mRNA expression analysis using quantitative real-time PCR (qRT-PCR), and an immunohistochemistry (IHC). According to the accumulated studies, MPOBA caused significant concentration- and time-dependent reductions in cell proliferation and cell migration and induced apoptosis in both CMC cell lines. In gene expression analysis, nine canine genes, including TP53, BCL-2, BAX, epidermal growth factor receptor (EGFR), snail transcription factor (SNAIL), snail-related zinc-finger transcription factor (SLUG), TWIST, E-cadherin, and N-cadherin, were investigated. The mRNA expression results revealed that MPOBA induced upregulation of TP53 and overexpression of the pro-apoptotic gene BAX, together with an inhibition of BCL-2. Moreover, MPOBA also suppressed the mRNA expression levels of SNAIL, EGFR, and N-cadherin and induced upregulation of E-cadherin, crucial genes related to the epithelial-to-mesenchymal transition (EMT). However, there was no significant difference in the IHC results of the expression patterns of vimentin (VT) and cytokeratin (CK) between MPOBA-treated and control CMC cells. In conclusion, the results of the present study suggested that MPOBA exhibited significant anticancer activity by inducing apoptosis in both CMCs via upregulation of TP53 and BAX and downregulation of BCL-2 relative mRNA expression. MPOBA may prove to be a potential candidate drug to be further investigated as a therapeutic agent for CMC.
An Evaluation of Paddy Rice as an Alternative Energy Source in Protein-Restricted Diets for Growing, Early-Finishing, and Late-Finishing Pigs
Authors: Wu Zijuan , Li Wenli , Wang Huixia , Li Yali
Published: January 2024

Three experiments were conducted to evaluate paddy rice as an alternative energy feedstuff in low-protein diets for pigs. In Experiment 1, a total of 400 growing pigs (20.68 ± 0.29 kg initial bodyweight), were randomly allocated four dietary treatments with 0, 10, 15, and 20% paddy rice for 30 days. Feeding 10% or 15% paddy rice had no adverse impacts on average daily gain (ADG) and feed to gain ratio (F:G), while the inclusion of 20% rice in diets significantly influenced the growth performance of pigs. In Experiment 2, 364 early-finishing pigs (42.25 ± 0.47 kg) were divided into four treatments with 0, 15, 20, and 25% paddy rice for 35 days. Feeding 15% or 20% paddy rice had no negative consequences on growth performance, while pigs fed with 25% rice had the lowest ADG and the greatest F:G. In Experiment 3, 364 late-finishing pigs (79.52 ± 1.28 kg) were divided into four treatments with 0, 20, 25, and 30% paddy rice for 60 days. Paddy rice can be included at up to 30% in diets without compromising growth performance, while feeding with 25% rice significantly improved the performance for pigs compared with the corn-fed control.
Seasonal Diet Changes and Trophic Links of Cold-Water Fish (Coregonus albula) within a Northern Lake Ecosystem
Authors: Berezina Nadezhda A. , Terentjev Piotr M. , Zubova Elena M. , Tsurikov Sergey M. , Maximov Alexey A. , Sharov Andrey N.
Published: January 2024

The seasonal feeding patterns of the cold-adapted fish, Coregonus albula, are poorly studied in high-latitude lakes but could provide insight for predicting the effects of global warming. We examined vendace’s diet composition, traced the carbon and nitrogen isotope ratios from producers to consumers in the food web, and estimated vendace’s trophic position in a subarctic lake (the White Sea basin). Results showed the vendace to be a typical euryphagous fish, but clear seasonal differences were found in the relative importance of plankton and benthos in the diet. The vendace consumed primarily benthic amphipods in the summer, planktonic cladocerans in the autumn, and copepods in the winter–spring (under ice); larvae of aquatic insects were the second-most important food items throughout the year. Because of the substantial proportion of fish embryos in its diet, the vendace had a trophic position similar to that of a predatory fish (perch). The Bayesian food source-mixing model revealed that the majority of vendace energy derives from planktonic copepods. The dominant Cyclops had the lowest carbon isotope values, suggesting a carbon-depleted diet typical for methanotrophic bacteria, as its probable food source was in a lake under ice. Understanding the feeding patterns of vendace provides information to better predict the potential biotic effects of environmental change on lake ecosystems.
Effects of Elevating Zinc Supplementation on the Health and Production Parameters of High-Producing Dairy Cows
Authors: Oconitrillo Maria , Wickramasinghe Janaka , Omale Shedrack , Beitz Donald , Appuhamy Ranga
Published: January 2024

This study’s objective was to determine the effects of increasing the dietary added zinc (Zn) on the milk production, milk somatic cell count (SCC), and immunoglobulin and antioxidant marker concentrations in the blood of dairy cows. Twelve Holstein cows (67 ± 2.5 days in milk) were assigned randomly to (1) a diet containing Zn–methionine at 76 mg/kg of DM (CTL) or (2) CTL top-dressed with about 21 mg/kg of DM extra Zn–methionine (+Zn) for 70 d. The concentrations of reduced (GSH) and oxidized (GSSG) glutathione, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and immunoglobulins in the blood were measured on d 0, 35, and 70. Compared to CTL, +Zn decreased the dry matter intake (DMI) throughout the trial and the milk yield (MY) during the first phase of feeding (0–35 d). It, however, increased the milk yield during the last phase (36–70 d). The +Zn tended to have lower and greater milk protein yields than CTL during the first and last feeding phases, respectively. The +Zn tended to decrease the SCC and was associated with lower plasma GSH: GSSG and lower serum SOD concentrations relative to CTL. The +Zn did not affect the immunoglobulins, MDA, or CAT. Despite the early DMI and MY reduction, the prolonged Zn–methionine supplementation at about 100 mg/kg of DM improved the milk yield, possibly as a result of the improved udder health of dairy cows.
In Vitro Assessment of Antifungal and Antibiofilm Efficacy of Commercial Mouthwashes against Candida albicans
Authors: Korbecka-Paczkowska Marzena , Karpiński Tomasz M.
Published: January 2024

Candida albicans is the most critical fungus causing oral mycosis. Many mouthwashes contain antimicrobial substances, including antifungal agents. This study aimed to investigate the in vitro activity of 15 commercial mouthwashes against 12 strains of C. albicans. The minimal inhibitory concentrations (MICs), minimal fungicidal concentrations (MFCs), and anti-biofilm activity were studied. MICs were determined by the micro-dilution method using 96-well plates, and MFCs were determined by culturing MIC suspensions on Sabouraud dextrose agar. Anti-biofilm activity was evaluated using the crystal violet method. The mouthwashes containing octenidine dihydrochloride (OCT; mean MICs 0.09–0.1%), chlorhexidine digluconate (CHX; MIC 0.12%), and CHX with cetylpyridinium chloride (CPC; MIC 0.13%) exhibited the best activity against C. albicans. The active compound antifungal concentrations were 0.5–0.9 µg/mL for OCT products and 1.1–2.4 µg/mL for CHX rinses. For mouthwashes with CHX + CPC, concentrations were 1.56 µg/mL and 0.65 µg/mL, respectively. Products with polyaminopropyl biguanide (polyhexanide, PHMB; MIC 1.89%) or benzalkonium chloride (BAC; MIC 6.38%) also showed good anti-Candida action. In biofilm reduction studies, mouthwashes with OCT demonstrated the most substantial effect (47–51.1%). Products with CHX (32.1–41.7%), PHMB (38.6%), BAC (35.7%), Scutellaria extract (35.6%), and fluorides + essential oils (33.2%) exhibited moderate antibiofilm activity. The paper also provides an overview of the side effects of CHX, CPC, and OCT. Considering the in vitro activity against Candida albicans, it can be inferred that, clinically, mouthwashes containing OCT are likely to offer the highest effectiveness. Meanwhile, products containing CHX, PHMB, or BAC can be considered as promising alternatives.
Coumarins in Spirit Beverages: Sources, Quantification, and Their Involvement in Quality, Authenticity and Food Safety
Authors: Oliveira-Alves Sheila , Lourenço Sílvia , Fernandes Tiago A. , Canas Sara
Published: January 2024

This comprehensive review is devoted to an under-exploited family of phenolic compounds, the coumarins, and the most relevant strands in which they are involved in some spirit beverages—wine spirit, brandy, whiskey, sugar-cane spirits (rum and cachaça) and grape marc spirit—with great importance worldwide in terms of production, trade and consumption. It gathers the key discoveries on the topic, considering the production process of each spirit beverage, and the related sources of coumarins (different kinds of wood used in the ageing stage), along with the factors that govern them and can influence the sensory properties of the aged beverages. An overview of the analytical methods available for their identification/quantification is also included, as well as the corresponding trends for the advancement of knowledge in this field. Moreover, the remarkable role of coumarins as nutraceuticals, their importance as chemical markers for authenticity purposes, and their relationship with the food safety of these spirit beverages are also addressed, highlighting the current gaps and issues, and providing clues for future research.
Factors That Impact the Dynamics and Effectiveness of Work Teams during the Implementation of Continuous Improvement Tools in the Manufacturing Industry: A Systematic Literature Review
Authors: Ramírez-Zavala Miriam Rubí , López-Guerrero Aída , Olivares-Fong Luz del Consuelo , Velázquez-Victorica Karla Isabel , Montoya-Alcaraz Marco
Published: January 2024

Currently, because of global competition, companies within the manufacturing industry must implement new organizational and production techniques to compete and stay active. The use of continuous improvement tools has become an opportunity and an effective strategy to achieve this. However, there is evidence that many continuous improvement programs are not successfully implemented due to a lack of information related to human management during the implementation of these projects in the manufacturing industry. In this context, the objective of this research is to identify factors influencing the dynamics and effectiveness of work teams for the implementation and development of continuous improvement tools in the manufacturing industry. A literature review was conducted using the PRISMA method, considering scientific articles related to the main factors that affect the dynamics and effectiveness of work teams in relation to the implementation of continuous improvement tools. From the review and evaluation of the studies, 60 factors were identified that affect the effectiveness of work teams in relation to continuous improvement within the manufacturing industry. Subsequently, a Pareto analysis was conducted on the critical success factors based on the number of occurrences in the analyzed literature, identifying 32 critical success factors considered vital for the implementation of continuous improvement projects. It is concluded that the factors arise from two common elements, the involvement of senior management and the company’s own culture. Therefore, to ensure the effectiveness of work teams and their continuous improvement projects, management must focus attention on this cultural change mainly by providing the necessary resources for the development of the project, establishing an adequate and effective reward system, and, most importantly, directing efforts towards staff empowerment.
AIoT Monitoring Technology for Optimal Fill Dam Installation and Operation
Authors: Kong Suk-Min , Yoo Chanho , Park Jaeim , Park Jae-Hyun , Lee Seong-Won
Published: January 2024

Fill dam structures are generally considered safe, but frequent heavy rainfall in recent years due to climate change has increased their risk of collapse. Technologies for the monitoring and safety management of these structures have attracted considerable attention, and methods to utilize technologies such as artificial intelligence (AI) and Internet of Things (IoT) for maintenance have been investigated. However, the measurement and communication processes of sensors used in the IoT technology are prone to measurement errors. Moreover, the communication environment and frequency range used by unlicensed operators vary within a country. Technologies for accurate interpretation of measurement results and optimal communication are required to address these issues. In this study, a technology for dam safety management and communication environment optimization was developed using AIoT (AI+IoT), and its field applicability was verified.
A Task-Adaptive Parameter Transformation Scheme for Model-Agnostic-Meta-Learning-Based Few-Shot Animal Sound Classification
Authors: Moon Jaeuk , Kim Eunbeen , Hwang Junha , Hwang Eenjun
Published: January 2024

Deep learning models that require vast amounts of training data struggle to achieve good animal sound classification (ASC) performance. Among recent few-shot ASC methods to address the data shortage problem regarding animals that are difficult to observe, model-agnostic meta-learning (MAML) has shown new possibilities by encoding common prior knowledge derived from different tasks into the model parameter initialization of target tasks. However, when the knowledge on animal sounds is difficult to generalize due to its diversity, MAML exhibits poor ASC performance due to the static initialization setting. In this paper, we propose a novel task-adaptive parameter transformation scheme called few-shot ASC. TAPT generates transformation variables while learning common knowledge and uses the variables to make parameters specific to the target task. Owing to this transformation, TAPT can reduce overfitting and enhance adaptability, training speed, and performance in heterogeneous tasks compared to MAML. In experiments on two public datasets on the same backbone network, we show that TAPT outperforms the existing few-shot ASC schemes in terms of classification accuracy, and in particular a performance improvement of 20.32% compared to the state-of-the-art scheme. In addition, we show that TAPT is robust to hyperparameters and efficient for training.
Design and Development of Rice Pot-Seedling Transplanting Machinery Based on a Non-Circular Gear Mechanism
Authors: Yang Jiajia , Zhou Maile , Yin Daqing , Yin Jianjun
Published: January 2024

Transplanting rice pot seedlings without damaging the roots, which promotes early tillering, is an effective measure to enhance rice yield and quality. This study aimed to obtain the mechanized-transplanting trajectory and attitude of rice pot seedlings by utilizing non-circular planetary-gear trains, focusing on the three key actions of rice pot-seedling transplanting: seedling picking, conveying, and planting. A lightweight and simplified rice pot-seedling transplanting machinery was designed, referring to the motion characteristics of artificially transplanting rice pot seedlings by first pulling them out and then planting them. Key technologies such as non-circular gear trains, the rice seedling supply system, the transmission system, and the rice seedling-picking device were studied, and their key components were designed and manufactured, resulting in the creation of two physical model machines: an ordinary ride type and a high-speed type. The seedling-picking test and field-transplanting test showed that the rice pot-seedling transplanting mechanism can accomplish the rice pot-seedling picking, rice conveying, and planting actions. The designed operation efficiency yielded a planting-depth qualification rate of over 92%, a seedling injury rate of less than 1.2%, and a missed-transplanting rate of less than 2%.