bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of fecal microRNA stability in healthy cats
Authors: Lyngby Janne G. , Kristensen Annemarie T. , Fredholm Merete , Nielsen Lise N. , Cirera Susanna
Published: June 2019

Background

Gastrointestinal (GI) cancer accounts for 14% of feline malignancies. There is a great need for reliable noninvasive diagnostic biomarkers to reach a timely diagnosis and initiate treatment. Fecal microRNAs (miRNAs) could be such a biomarker and have shown great potential in colorectal screening in people but have yet to be investigated in cats.

Objectives

We aimed to evaluate the presence and stability of feline fecal miRNA under different storage conditions (room temperature [RT], 4, and −20°C) and to evaluate the expression levels of specific fecal miRNAs collected on three separate days (days 1, 4, and 7) in healthy cats.

Methods

Healthy cats were prospectively recruited. Fecal samples were collected, aliquoted, and stored for 24 hours at RT and then transferred to −20°C, stored for 24 hours at 4°C and then transferred to −20°C, or were immediately placed at −20°C on day 1 or at −20°C on days 4 and 7 postcollection. Expression of 22 miRNAs was investigated using quantitative real‐time PCR.

Results

Ten miRNA assays worked well, and one, let‐7b, was used for normalization. No differences in miRNA expression were seen between the three storage temperatures for the nine miRNAs investigated. Only miR‐26a showed a significant increase in expression between samples of days 1 and 7. The rest of the miRNAs levels were stable over time.

Conclusions

Fecal miRNA can be isolated from healthy cats. The expression was stable at different temperatures and for most of the miRNAs over time. Prospective studies evaluating fecal miRNA as biomarkers in cats with GI neoplasia are warranted.
Assessment of hydromorphone and dexmedetomidine for emesis induction in cats
Authors: Nystrom Michael R. , Odunayo Adesola , Okafor Chika C.
Published: June 2019

Objective

To compare the efficacy of hydromorphone and dexmedetomidine at inducing emesis in cats.

Design

Prospective, blinded, randomized crossover study.

Setting

Veterinary university teaching hospital.

Animals

12 healthy purpose‐bred cats.

Interventions

Cats were randomly assigned to receive hydromorphone (0.1 mg/kg, subcutaneously) or dexmedetomidine (7 μg/kg, IM). Following administration, the incidences of emesis, number of emetic events, signs of nausea (hypersalivation, lip licking), temperature, heart rate, respiratory rate, and sedation score were recorded for 6 hours.

Measurements and Main Results

Emesis was successful in 9 of 12 (75%) cats when treated with hydromorphone and in 7 of 12 (58%) cats when treated with dexmedetomidine (P = 0.67). Dexmedetomidine was more likely to cause sedation than hydromorphone (P < 0.001). Heart rate in cats was significantly decreased at 1 and 2 hours post‐hydromorphone (P = 0.003, 0.014, respectively) and at 1, 2, 3, 5, 6 hours post‐dexmedetomidine (P = 0.001, 0.003, 0.038, 0.013, 0.001, respectively). Cats were more likely to develop an increase in body temperature with hydromorphone administration although this was not clinically significant.

Conclusions

Results of the present study indicate that hydromorphone is an effective alternative to dexmedetomidine for the induction of emesis in cats. Hydromorphone appears to cause less sedation and less decrease in heart rate. Further investigation into the most adequate dose of hydromorphone for optimizing emesis is warranted.
SOLUTION PARAMETER EFFECT ON POLYSULFONE FIBERS VIA ELECTROSPINNING: FABRICATION, CHARACTERIZATION AND WATER FLUX PROPERTY
Authors: Yong Chee Tan , Mohd Yusoff Abdull Rahim , Rahmat Nur Atika , Malek Nik Ahmad Nizam Nik
Published: June 2019

This research investigates the solution parameters, i.e., polymer concentration and addition of manganese (IV) oxide (MnO2) nanoparticles, for the fabrication of polysulfone (PSF) fibers via electrospinning. Initially, PSF was dissolved in N,N-dimethylformamide (DMF) solvent and electrospun fibers with different morphologies were obtained using the range of PSF concentration of 10% (w/v) to 25% (w/v). Subsequently, PSF with the concentration of 20% (w/v) (denoted as PSF(20%)) was chosen to blend with 0.2% (w/v) of MnO2 as it gave the most stable electrospinnability and uniform fiber diameter. The fabricated electrospun PSF(20%) and PSF(20%)-MnO2 fibrous membranes were characterized to determine the morphology, wettability property, zeta potential, and tensile strength. The presence of MnO2 improved tensile strength as it reduced the fiber diameter that eventually made a more compact fiber mat membrane. The results of contact angle confirmed that the fabricated fiber exhibited more hydrophobic property in the presence of MnO2 nanoparticles. Thus, it reduced the pure water flux of PSF fiber membrane. The more hydrophobic nature of the proposed nanofiber might be useful in enhancing the application of PSF fiber in oil-water separation process.
FUNCTIONAL ANALYSIS OF THE PERSICARIA MINOR SESQUITERPENE SYNTHASE GENE PROMOTER IN TRANSGENIC ARABIDOPSIS THALIANA
Authors: Omar Aimi Farehah , Ismail Ismanizan
Published: June 2019

Sesquiterpene synthase is an enzyme involved in sesquiterpene biosynthesis which catalyzed sesquiterpene formation from farnesyl diphosphate (FDP). In this research, the sesquiterpene synthase promoter (PmSS) was isolated from Persicaria minor (P.minor) to identify the functional region of the promoter and possible cis - regulatory element involved in the regulation of sesquiterpene synthase gene. Various putative cis - regulatory element involved in environmental stress and hormones were identified on PmSS promoter. The PmSS promoter and three series of deletion promoter were fused to β-glucuronidase (gus) gene and transformed into Arabidopsis thaliana. This study showed PmSS promoter was regulated in a developmental -specific manner and response to wounding, drought, heat, abscisic acid (ABA) and methyl jasmonate (MeJa) treatment. The results revealed the existence of cis regulatory elements that control the regulation of promoter activity in a developmental specific manner at -1758 to -1078 promoter sequences. The presence of cis-element acting as a repressor is expected to be present at the promoter between -1540 to -1078 bp. The region from -1078 to -855 was critical for maximal PmSS promoter activity. Deletion of promoter region from -1758 to -855 induced regulation of promoter in an organ-specific manner. Drought stress treatment did not induced GUS activity in deleted ABRE motif construct, suggested that ABRE motif is essential cis element during drought stress.
FOAM STABILITY PERFORMANCE ENHANCED WITH RICE HUSK ASH NANOPARTICLES
Authors: Kai Jie Chuah , Jaafar Mohd Zaidi , Wan Sulaiman Wan Rosli
Published: June 2019

Rice husk ash (RHA) has been recently used as a source of silica (SiO2) production due to its high silica content. Besides, high purity silica nano-powder has been successfully synthesized from RHA and employed in various industries including electronic component manufacturing and fillers in polymers. Meanwhile, silica nanoparticles has been widely used in the application of Enhanced Oil Recovery (EOR). This is due to its ability in enhancing the foam stability besides modifying the wettability of the rocks in the formation. However, the synthesis of silica nanoparticles from RHA for the application in big scale operation such as EOR using conventional method is energy and time consuming. Therefore, the objective of this work is to study the effectiveness of using nano-sized rice husk ash (nano-RHA) as an additive to stabilize normal gas generated surfactant foam used in EOR. In order to decrease the size of the RHA into nano range, planetary ball mill was used in both dry grinding and wet grinding. Different surfactants including anionic and non-ionic were then used to study the polydispersity index of the dispersion and the hydrodynamic diameter using dynamic light scattering in dilute suspension. Besides, the nano-RHA was also characterized using FESEM, EDX, XRD and the change in specific area after grinding process was studied using BET. The foamability of different surfactants were then studied using minor concentration of nano-RHA. Next, the concentration of the nano-RHA was varied from 0.1wt% to 0.9wt% in normal gas bulk foam stability test using the suitable surfactant, the texture of foam was observed as well. Apart from that, the effect of oil on bulk foam was also studied. Finally, the result was compared using pure silica nanoparticles as the foam addictive at the same variation of concentrations. Dispersion stability tests showed that both anionic and non-ionic surfactants can be used to disperse nano-RHA in water. Moreover, in the presence of 0.9wt% of nano-RHA concentration, the bulk foam stability test results revealed that the sodium dodecyl sulfate (SDS) bulk foam half-life increased by 17.9% without the presence of oil, and gave an increment of 20.7% half-life in the presence of oil. Therefore, the study showed a potential of utilizing nano-RHA in stabilizing bulk foam.
Reviewer Acknowledgements
Authors: Johnson Patricia
Published: June 2019

Studies in Media and Communication (SMC) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether SMC publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 7, Number 1Adriana Car-Mihec, University of Rijeka, CroatiaAndreas Veglis, Aristotle University of Thessaloniki, GreeceAntonio García Gómez, University of Alcalá de Henares, SpainAslı Sezgin, Osmaniye Korkut Ata University, TurkeyBegoña Montero-Fleta, Universitat Poltècnica de València, SpainCarmen Pérez-Sabater, Universitat Politècnica de València, SpainDiego Santos Vieira de Jesus, International Relations, BrazilJavier Serrano Puche, University of Navarra, SpainJoerg Tropp, Pforzheim University, GermanyNuran E. Isik, Izmir University of Economics, TurkeyWilliam Thomas Howe, University of Oklahoma, USAYoung Joon Lim, University of Texas Rio Grande Valley, USAYuan Gao, Curtin University, Australia Patricia JohnsonEditorial AssistantOn behalf of,The Editorial Board of Studies in Media and CommunicationRedfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USAURL: http://smc.redfame.com
Stimulation of nicotinic receptors in the suprachiasmatic nucleus results in a higher number of growing follicles and ova shed
Authors: Vieyra Elizabeth , Ramírez Deyra A. , Linares Rosa , Rosas Gabriela , Domínguez Roberto , Morales‐Ledesma Leticia
Published: June 2019

New Findings

What is the central question of this study?

What is the role of the nicotinic system of the suprachiasmatic nucleus (SCN) in the regulation of follicular growth and ovulation?

What is the main finding and its importance?

The stimulation of the nicotinic system of the pro‐oestrus rat SCN results in an increase in the number of ova shed, in the number of growing ovarian follicles and in the secretion of oestradiol.

The timing of the preovulatory luteinizing hormone surge that leads to ovulation depends to a large extent on a functional circadian clock that is localized in the suprachiasmatic nucleus (SCN). The activities of the SCN are regulated by several neurotransmitter systems, including the muscarinic system. Given that acetylcholine binds to muscarinic (mAChRs) and nicotinic (nAChRs) receptors, in the present study, we analysed the effects of unilaterally stimulating nAChRs in the left or right SCN. Stimulation treatment was administered in rats in pro‐oestrus at 09.00 or 19.00 h by injecting 0.3 µl of a nicotine solution (200 µm). The effects of the stimulation were assessed by evaluating the number of ova shed, the number of ovarian follicles, and the levels of oestradiol and progesterone in serum 24 h after treatment. We observed that regardless of the time (4 h after lights on, 09.00 h, or immediately after lights off, 19.00 h) or the side of the SCN treated, the unilateral microinjection of nicotine resulted in a higher number of ova shed and higher number of growing follicles in the ovaries as well as higher oestradiol serum levels. When the nicotine microinjection treatment failed to reach the SCN, the oestradiol levels in serum were similar to those of animals treated with vehicle solution. Based on the current results, we suggest that during pro‐oestrus, the nicotinic neuronal information in the SCN modulates follicular growth and ovulation in a stimulatory manner.
Comments on some Typhlodrominae from Pakistan (Acari: Phytoseiidae): re-descriptions, new synonymies, new combinations, and a key to the species
Authors: Kamran Muhammad , Halliday Bruce , Bashir Muhammad Hamid , Honey Sabyan Faris , Afzal Muhammad
Published: June 2019

The fauna of Typhlodrominae in Pakistan (Acari: Phytoseiidae) includes five genera and 54 nominal species, reported from different agroecosystems and climatic zones of the country. The most diverse genera are Typhlodromus Scheuten (37 species) and Kuzinellus Wainstein (13 species). Two species of Chanteius Wainstein, and one species each of the genera Cydnoseius Muma and Neoseiulella Muma have been reported. Two species previously placed in Typhlodromus are here transferred to Chanteius, due to the absence of seta s6 and presence of Z1. Two species, T. (Anthoseius) hadii Chaudhri and T. (Anthoseius) maracus (Chaudhri), are re-described with improved descriptions and illustrations. Four species previously placed in Typhlodromus are considered as synonyms of Cydnoseius negevi (Swirski & Amitai 1961). Typhlodromus (Anthoseius) divergentis (Chaudhri, Akbar & Rasool) is considered as a synonym of T. (Anthoseius) egypticus El-Badry, and T. husaini Khan is considered as species inquirenda. A provisional key to the species of Typhlodrominae in Pakistan is provided.
Effective dynamic properties of random complex media with spherical particles
Authors: Alam M. Mahbub , Pinfield Valerie J. , Luppé Francine , Maréchal Pierre
Published: June 2019

The effective dynamic bulk modulus and density are presented for random media consisting of particles in a viscous host fluid, using a core-shell, self-consistent effective medium model, under the large compressional wavelength assumption. These properties are relevant to acoustic or dynamic processes in nano- and micro-particle fluids including particle density determination, resonant acoustic mixing, and acoustic characterisation. Analytical expressions are obtained for the effective bulk modulus and mass density, incorporating the viscous nature of the fluid host into the core-shell model through wave mode conversion phenomena. The effective density is derived in terms of particle concentration, particle and host densities, particle size, and the acoustic and shear wavenumbers of the liquid host. The analytical expressions obtained agree with prior known results in the limit of both static and inviscid cases; the ratio of the effective bulk modulus to that of the fluid is found to be quasi-static. Numerical calculations demonstrate the dependence of the effective mass density on frequency, particle size (from nano- to micro-regime), and concentration. Herein it is demonstrated both theoretically and numerically that the viscosity, often neglected in the literature, indeed plays a significant role in the effective properties of nanofluids.
Integrating vision and echolocation for navigation and perception in bats
Authors: Danilovich S. , Yovel Y.
Published: June 2019

Egyptian fruit bats integrate vision and echolocation in a task-dependent manner.
Computing hubs in the hippocampus and cortex
Authors: Clawson Wesley , Vicente Ana F. , Ferraris Maëva , Bernard Christophe , Battaglia Demian , Quilichini Pascale P.
Published: June 2019

Neurons in an ensemble perform distinct information processing operations but dynamically switch their roles across states.
A soft ring oscillator
Authors: Preston Daniel J. , Jiang Haihui Joy , Sanchez Vanessa , Rothemund Philipp , Rawson Jeff , Nemitz Markus P. , Lee Won-Kyu , Suo Zhigang , Walsh Conor J. , Whitesides George M.
Published: June 2019

An elastomeric device converts a constant input pressure into multiple oscillating, temporally offset output pressures.
From micrographs to microsatellites in one bold step
Authors: Naxerova Kamila
Published: June 2019

Machine learning predicts microsatellite instability from histology.
Tyrosine Phosphorylation as a Widespread Regulatory Mechanism in Prokaryotes
Authors: Getz Landon J. , Runte Cameron S. , Rainey Jan K. , Thomas Nikhil A.
Published: June 2019

Phosphorylation events modify bacterial and archaeal proteomes, imparting cells with rapid and reversible responses to specific environmental stimuli or niches. Phosphorylated proteins are generally modified at one or more serine, threonine, or tyrosine residues. Within the last ten years, increasing numbers of global phosphoproteomic surveys of prokaryote species have revealed an abundance of tyrosine-phosphorylated proteins.
Genome Sequences of Escherichia Bacteriophages Isolated from Raw Wastewater
Authors: Keely Scott P. , Herrmann Michael P. , Korajkic Asja , Brinkman Nichole E. , McMinn Brian R. , Fout G. Shay , Villegas Eric N.
Published: June 2019

Somatic coliphages are alternative indicators of fecal pollution and attractive surrogates for viral pathogens. Here, we report the draft genome sequences of three replicate plaques from a novel
Myoviridae
bacteriophage isolated from raw wastewater. These genomes were similar to felix01virus phage and are predicted to contain up to 148 protein-coding genes.
Draft Whole-Genome Sequences of 16 Campylobacter jejuni Isolates Obtained from Wild Birds
Authors: Carraro Lisa , Marotta Francesca , Janowicz Anna , Patavino Claudio , Piccirillo Alessandra
Published: June 2019

The draft genome sequences of 16
Campylobacter jejuni
isolates obtained from wild birds are presented in this study. These genomes provide insights into the genetic features of
C. jejuni
isolates from wild birds, which are considered common hosts of this microorganism but have scarcely been investigated to date.
Draft Genome Sequence of Zygosaccharomyces mellis CA-7, Isolated from Honey
Authors: Shiwa Yuh , Kanesaki Yu , Ishige Taichiro , Mura Kiyoshi , Hori Taichi , Tamura Tomoko
Published: June 2019

In this study, we report the draft genome sequence of
Zygosaccharomyces mellis
CA-7, isolated from purchased honey imported from Canada. The 10.19-Mb genome contains 4,963 gene models. To our knowledge, this annotated genome sequence is the first from the species
Z. mellis
and will contribute to a better understanding of the osmotolerance of microorganisms in high-sugar products.
Using biomarkers to predict clinical outcomes in multiple sclerosis
Authors: Castle Daniel , Wynford-Thomas Ray , Loveless Sam , Bentley Emily , Howell Owain W , Tallantyre Emma C
Published: June 2019

Long-term outcomes in multiple sclerosis (MS) are highly varied and treatment with disease-modifying therapies carries significant risks. Finding tissue biomarkers that can predict clinical outcomes would be valuable in individualising treatment decisions for people with MS. Several candidate biomarkers—reflecting inflammation, neurodegeneration and glial pathophysiology—show promise for predicting outcomes. However, many candidates still require validation in cohorts with long-term follow-up and evaluation for their independent contribution in predicting outcome when models are adjusted for known demographic, clinical and radiological predictors. Given the complexity of MS pathophysiology, heterogeneous panels comprising a combination of biomarkers that encompass the various aspects of neurodegenerative, glial and immune pathology seen in MS, may enhance future predictions of outcome.
Enhanced needle visibility by microbubbles generated with negative pressure using an in-plane technique
Authors: Liu Yong , Sun Xingxing , Qian Wei , Liu Wantao , Mei Wei
Published: June 2019

Background and objectives

Our previous work found that needle visibility could be improved by introducing microbubbles into needles. The primary aim of this study was to test the hypothesis that the negative pressure method is superior to two other methods for enhancing needle visibility by introducing microbubbles into needles. The secondary aim was to evaluate the impacts of three factors on the visibility of microbubble-filled needles.

Methods

In the first phase, three methods, including the negative pressure method, the mixing method and commercialized microbubbles, were applied to generate microbubbles inside needles for comparison of visibility in a porcine meat model. In the second phase, three factors were tested with a 2×5×5 factorial design to explore their influence on the visibility of microbubble-filled needles. The three factors included types of needles, insertion angles and types of contents inside needles. Needles filled with saline without microbubbles were used as the control in both phases. Insertion videos were recorded, and ultrasound images of needles were captured for the objective visibility analysis.

Results

Needle visibility was highest in the negative pressure method group (p<0.001). Needle visibility was mainly determined by insertion angles (p<0.001). Microbubble-filled needles were more visible than control needles at 40°, 50° and 60° (p<0.001, p<0.001 and p<0.001, respectively).

Conclusions

Needle visibility can be significantly improved by microbubbles generated with the negative pressure method when insertion angles are 40°, 50° and 60° in porcine meat.
Characteristics of CO2, water vapor, and energy exchanges at a headwater wetland ecosystem of the Qinghai Lake
Authors: Cao Shengkui , Cao Guangchao , Chen Kelong , Han Guangzhao , Liu Ying , Yang Yufan , Li Xiaodong
Published: June 2019

Ecosystem carbon dioxide (CO2), water vapor, and heat exchanges in alpine wetlands on the Qinghai–Tibetan Plateau are not comprehensively understood. Thus, we studied variability of net ecosystem CO2 exchange (NEE), ecosystem respiration (Re), gross primary production (GPP), evapotranspiration (ET), and heat fluxes over a headwater wetland ecosystem in the Qinghai Lake region. Results showed that the headwater wetland ecosystem was net CO2 absorption on the annual scale, in which monthly NEE, GPP, and Re in two consecutive years varied from −165.16 to 93 g CO2 m−2 mo−1, 6.66 to 384.45 g CO2 m−2 mo−1, and 6.9 to 232.02 g CO2 m−2 mo−1, respectively. The monthly ET from June to September was smaller than precipitation; these results reversed in the remaining months. Annual ET was 362.1 mm in 2015 and 324.96 mm in 2016. The net radiation (Rn), sensible heat (H), latent heat (LE), and ground heat (G) fluxes showed similar monthly patterns. Values of monthly average half-hour Rn, H, LE, and G at the daytime showed Rn > LE > H > G, and the time of the monthly half-hour G peak obviously lagged the Rn, H, and LE. Monthly average Bowen ratios were <1 from May to October, but it reversed in the rest of the months.
Performance evaluation of hybrid modulation techniques for high speed radio over fiber communication system
Authors: Shantha Selva Kumari R. , Seyatha K.
Published: June 2019

In Radio over Fiber (RoF) technology, the optical network capacity is integrated with the wireless network. The main objective of this work is to enhance the performance behavior of optical fiber communication system in terms of BER, Received Power and Q-Factor. The proposed combined modulation technologies, such as Pulse Amplitude Frequency Modulation (PAFM), Differential Phase Shift Keying Amplitude Modulation (DPSKA), Offset Quadrature Phase Shift Keying Amplitude Modulation (OQPSKA), Quadrature Amplitude Frequency Modulation (QAFM), Frequency Phase Modulation (FPM) and Pulse Amplitude Phase Modulation (PAPM), are used to meet this objective. The input data are used for modulating the electrical carrier signal with a frequency of 250 and 255[Formula: see text]GHz. This hybrid modulation technique obtained the minimum BER of about [Formula: see text] in FPM technique. The maximum Q-Factor of 33 is obtained in QAFM technique. The obtained maximum received power is 0.79[Formula: see text]mW in OQPSKA technique.
Approximating Robust Parameterized Submodular Function Maximization in Large-Scales
Authors: Yang Ruiqi , Xu Dachuan , Jiang Yanjun , Wang Yishui , Zhang Dongmei
Published: June 2019

We study a robust parameterized submodular function maximization inspired by [Mitrović, S, I Bogunovic, A Norouzi-Fard and Jakub Tarnawski (2017). Streaming robust submodular maximization: A partitioned thresholding approach. In Proc. NIPS, pp. 4560–4569] and [Bogunovic, I, J Zhao and V Cevher (2018). Robust maximization of nonsubmodular objectives. In Proc. AISTATS, pp. 890–899]. In our setting, given a parameterized set function, there are two additional twists. One is that elements arrive in a streaming style, and the other is that there are at most [Formula: see text] items deleted from the algorithm’s memory when the stream is finished. The goal is to choose a robust set from the stream such that the robust ratio is maximized. We propose a two-phase algorithm for maximizing a normalized monotone robust parameterized submodular function with a cardinality constraint and show the robust ratio is close to a constant as [Formula: see text]. In the end, we empirically demonstrate the performance of our algorithm on deletion robust support selection problem.
Asynchronous Algorithms for Computing Equilibrium Prices in a Capital Asset Pricing Model
Authors: Tong Jun , Hu Jian-Qiang , You Jianxin
Published: June 2019

In this paper, we extend the work of [Tong, J, J Hu and J Hu (2017). Computing equilibrium prices for a capital asset pricing model with heterogeneous beliefs and margin-requirement constraints. European Journal of Operational Research, 256(1), 24–34] and develop various asynchronous algorithms to calculate the equilibrium asset prices in a heterogeneous capital asset pricing model. These asynchronous algorithms are based on different asynchronous updating schemes such as delayed updating, cyclic updating, fixed-length updating and random updating. In addition to potential benefits of improving computational efficiency, these asynchronous updating schemes also reflect several scenarios in financial markets in which investors may receive asset pricing information with various degrees of delays and their preferences on how and when to rebalance their portfolios may also be different. The proofs for the convergence of these algorithms are given. Numerical experiments are also provided to compare these algorithms and they show that these asynchronous algorithms work quite well.
Real-Time Tracking of Structural Stiffness Reduction with Unknown Inputs, Using Self-Adaptive Recursive Least-Square and Curvature-Change Techniques
Authors: Askari Mohsen , Yu Yang , Zhang Chunwei , Samali Bijan , Gu Xiaoyu
Published: June 2019

In this paper, a new computationally efficient algorithm is developed for online and real-time identification of time, location, and severity of abrupt changes in structural stiffness as well as the unknown inputs such as earthquake signal. The proposed algorithm consists of three stages and is based on self-adaptive recursive least-square (RLS) and curvature-change approaches. In stage 1 (intact structure), a simple compact RLS is hired to estimate the unknown parameters and input of the structure such as stiffness and earthquake. Once the damage has occurred, its time and location are identified in stage 2, using two robust damage indices which are based on the structural jerk response and the error between measured and estimated responses of structure from RLS. Finally, the damage severity as well as the unknown excitations are identified in the third stage (damaged structure), using a self-adaptive multiple-forgetting-factor RLS. The method is validated through numerical and experimental case studies including linear and nonlinear buildings, a truss structure, and a three-story steel frame with different excitations and damage scenarios. Results show that the proposed algorithm can effectively identify the time-varying structural stiffness as well as unknown excitations with high computational efficiency, even when the measured data is contaminated with different levels of noise. In addition, as no optimization method is used here, it can be applied to real-time applications with computational efficiency.
The attractor of piecewise expanding maps of the interval
Authors: Magno Gianluigi Del , Dias João Lopes , Duarte Pedro , Gaivão José Pedro
Published: June 2019

We consider piecewise expanding maps of the interval with finitely many branches of monotonicity and show that they are generically combinatorially stable, i.e. the number of ergodic attractors and their corresponding mixing components do not change under small perturbations of the map. Our methods provide a topological description of the attractor and give an elementary proof of the density of periodic orbits.
New insights on subsurface energy resources in the Southern North Sea Basin area
Authors: Doornenbal J. C. , Kombrink H. , Bouroullec R. , Dalman R. A. F. , De Bruin G. , Geel C. R. , Houben A. J. P. , Jaarsma B. , Juez-Larré J. , Kortekaas M. , Mijnlieff H. F. , Nelskamp S. , Pharaoh T. C. , Ten Veen J. H. , Ter Borgh M. , Van Ojik K. , Verreussel R. M. C. H. , Verweij J. M. , Vis G.-J.
Published: June 2019

The Southern North Sea Basin area, stretching from the UK to the Netherlands, has a rich hydrocarbon exploration and production history. The past, present and expected future hydrocarbon and geothermal exploration trends in this area are discussed for eight key lithostratigraphic intervals, ranging from the Lower Carboniferous to Cenozoic. In the period between 2007 and 2017, a total of 95 new hydrocarbon fields were discovered, particularly in Upper Carboniferous, Rotliegend and Triassic reservoirs. Nineteen geothermal systems were discovered in the Netherlands onshore, mainly targeting aquifers in the Rotliegend and Upper Jurassic/Lower Cretaceous formations. Although the Southern North Sea Basin area is mature in terms of hydrocarbon exploration, it is shown that with existing and new geological insights, additional energy resources are still being proven in new plays such as the basal Upper Rotliegend (Ruby discovery) for natural gas and a new Chalk play for oil. It is predicted that hydrocarbon exploration in the Southern North Sea Basin area will probably experience a slight growth in the coming decade before slowing down, as the energy transition further matures. Geothermal exploration is expected to continue growing in the Netherlands onshore as well as gain more momentum in the UK.
Pyroptosis engagement and bladder urothelial cell-derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after uropathogenic E. coli infection
Authors: Wu Zonglong , Li Yan , Liu Qinggang , Liu Yaxiao , Chen Lipeng , Zhao Hongda , Guo Hongda , Zhu Kejia , Zhou Nan , Chai Toby C. , Shi Benkang
Published: June 2019

The specific regulatory mechanism of bladder urothelial barrier dysfunction after infection with uropathogenic Escherichia coli (UPEC) is still unclear. The cross talk between bladder urothelial cells and mast cells may play an important role during UPEC infection. In this study, the pyroptosis of urothelial cells was investigated after UPEC infection both in vivo and in vitro. The levels of IL-1β and IL-18 in exosomes derived from bladder urothelial cells after UPEC infection were detected. The role of these processes in the recruitment and activation of mast cells was measured. The mechanism of mast cell-induced disruption of bladder epithelial barrier function was also assessed. We found that UPEC infection induced pyroptosis of bladder urothelial cells and led to the release of IL-1β and IL-18 in the form of exosomes, which promoted the migration of mast cells. Tryptase secreted by mast cells aggravated the damage to the barrier function of the bladder urothelium by acting on protease-activated receptor 2 (PAR2). Inhibition of pyroptosis or the tryptase-PAR2 axis reduced the disruption of bladder urothelial barrier function and decreased the bacterial burden. The present study supports a novel mechanism by which pyroptosis-dependent release of exosomes from bladder urothelial cells activates mast cells and regulates bladder urothelial barrier function during UPEC infection.
Neurohumoral interactions contributing to renal vasoconstriction and decreased renal blood flow in heart failure
Authors: Ramchandra Rohit , Xing Daniel T. , Matear Marcus , Lambert Gavin , Allen Andrew M. , May Clive N.
Published: June 2019

In heart failure (HF), increases in renal sympathetic nerve activity (RSNA), renal norepinephrine spillover, and renin release cause renal vasoconstriction, which may contribute to the cardiorenal syndrome. To increase our understanding of the mechanisms causing renal vasoconstriction in HF, we investigated the interactions between the increased activity of the renal nerves and the renal release of norepinephrine and renin in an ovine pacing-induced model of HF compared with healthy sheep. In addition, we determined the level of renal angiotensin type-1 receptors and the renal vascular responsiveness to stimulation of the renal nerves and α1-adrenoceptors. In conscious sheep with mild HF (ejection fraction 35%–40%), renal blood flow (276 ± 13 to 185 ± 18 mL/min) and renal vascular conductance (3.8 ± 0.2 to 3.1 ± 0.2 mL·min−1·mmHg−1) were decreased compared with healthy sheep. There were increases in the burst frequency of RSNA (27%), renal norepinephrine spillover (377%), and plasma renin activity (141%), whereas the density of renal medullary angiotensin type-1 receptors decreased. In anesthetized sheep with HF, the renal vasoconstrictor responses to electrical stimulation of the renal nerves or to phenylephrine were attenuated. Irbesartan improved the responses to nerve stimulation, but not to phenylephrine, in HF and reduced the responses in normal sheep. In summary, in HF, the increases in renal norepinephrine spillover and plasma renin activity are augmented compared with the increase in RSNA. The vasoconstrictor effect of the increased renal norepinephrine and angiotensin II is offset by reduced levels of renal angiotensin type-1 receptors and reduced renal vasoconstrictor responsiveness to α1-adrenoceptor stimulation.
The contribution of collecting duct NOS1 to the concentrating mechanisms in male and female mice
Authors: Mendoza Luciano D. , Hyndman Kelly A.
Published: June 2019

The collecting duct (CD) concentrates the urine, thereby maintaining body water volume and plasma osmolality within a normal range. The endocrine hormone arginine vasopressin acts in the CD to increase water permeability via the vasopressin 2 receptor (V2R)-aquaporin (AQP) axis. Recent studies have suggested that autocrine factors may also contribute to the regulation of CD water permeability. Nitric oxide is produced predominantly by nitric oxide synthase 1 (NOS1) in the CD and acts as a diuretic during salt loading. The present study sought to determine whether CD NOS1 regulates diuresis during changes in hydration status. Male and female control and CD NOS1 knockout (CDNOS1KO) mice were hydrated (5% sucrose water), water deprived, or acutely challenged with the V2R agonist desmopressin. In male mice, water deprivation resulted in decreased urine flow and increased plasma osmolality, copeptin concentration, and kidney AQP2 abundance independent of CD NOS1. In female control mice, water deprivation reduced urine flow, increased plasma osmolality and copeptin, but did not significantly change total AQP2; however, there was increased basolateral AQP3 localization. Surprisingly, female CDNOS1KO mice while on the sucrose water presented with symptoms of dehydration. Fibroblast growth factor 21, an endocrine regulator of sweetness preference, was significantly higher in female CDNOS1KO mice, suggesting that this was reducing their drive to drink the sucrose water. With acute desmopressin challenge, female CDNOS1KO mice failed to appropriately concentrate their urine, resulting in higher plasma osmolality than controls. In conclusion, CD NOS1 plays only a minor role in urine-concentrating mechanisms.
Stiffness as a control factor for object manipulation
Authors: Kennedy Scott D. , Schwartz Andrew B.
Published: June 2019

During manipulation, force is exerted with the expectation that an object will move in an intended manner. This prediction is a learned coordination between force and movement. Mechanically, impedance is a way to describe this coordination, and object interaction could be anticipated by setting impedance before the hand moves the object. This strategy would be especially important at the end of a reach, because feedback is ineffective for rapid force changes. Since mechanical impedance is not subject to the time delays of feedback, it can, if set properly, produce the desired motion on impact. We examined this possibility by instructing subjects to move a handle to a specific target position along a track. The handle was locked in place until the subject exerted enough force to cross a threshold; the handle was then released abruptly to move along the track. We hypothesized that this ballistic release task would encourage subjects to modify impedance in anticipation of the upcoming movement and found that one component of impedance, stiffness, varied in a way that matched the behavioral demands of the task. Analysis suggests that this stiffness was set before the handle moved and governed the subsequent motion. We also found separate components of muscle activity that corresponded to stiffness and to changes in force. Our results show that subjects used a robust and efficient strategy to coordinate force and displacement by modulating muscle activity in a way that was behaviorally relevant in the task.

NEW & NOTEWORTHY The arm can behave like a spring, and this mechanical behavior can be advantageous in situations requiring rapid changes in force and/or displacement. Selection of a proper “virtual” spring before the occurrence of a rapid transient could facilitate a desired responsive movement. We show that these spring-like arm mechanics, set in anticipation of an instantaneous force change, function as an efficient strategy to control movement when feedback is ineffective.
Analysis of NMR Spectrometer Receiver Noise Figure
Authors: Andris Peter , Emery Earl F. , Frollo Ivan
Published: June 2019

This article describes the measurement and evaluation of the system noise figure of a nuclear magnetic resonance spectrometer. A method was used which involved the console of the spectrometer, calibrated for real voltage (in volts), and used for noise signal digitisation and measurement. The resulting digitised signal was exported and processed, and the root mean square value calculated. This value was utilised for the noise power calculation, which was compared with the theoretical value and the noise figure calculated. The method presented enables analysis of the bandwidth of the noise. Many of the equations that are commonly used for signal processing have been derived for the specific task and require verification. We have verified the technique using a commercial console with two preamplifier pairs. The experimental data agree well with the theoretical values, confirming that the presented method is a valid, simple, and fast tool for the inspection of the spectrometer receiver.
Maneuverability Analysis of a Novel Portable Modular AUV
Authors: Wang Xiaoming , Liang Shengguo
Published: June 2019

ZFAUV is a novel portable modular AUV. There are four fixed thrusters at tail, and two tunnel thrusters are set at front. The maneuverability of ZFAUV is relatively high. It can turn around in situ, move lateral or move up/down vertical. The yaw and pitch can be controlled by tunnel thrusters or differential control of tail thrusters, but differential control will reduce the forward force. Different from propeller‐rudder AUVs, the turning radius is related to speed forward: the smaller the speed forward, the smaller the turning radius. The minimum turning radius tends to be zero. The mathematical model is built first; then CFD is used to predict the thrust and torque of tail thrusters and tunnel thrusters. Through numerical simulation, zigzag maneuver analysis in horizontal plane, and trapezoidal steering maneuver analysis in vertical plane, the maneuverability of ZFAUV is obtained. The maneuverability of ZFAUV becomes worse with the increase of speed. The maneuverability of differential control is better than that of tunnel control. In the case of specific thrust distribution of tail thrusters and tunnel thrusters, ZFAUV can turn around in situ (the maximum angular velocity is about 24.1°/s), move lateral or move up/down vertical (the maximum velocity is about 0.4m/s). Finally, an example, PID parameters tuning, is given to illustrate the application of maneuverability analysis. The dynamic performance of ZFAUV can be quickly and accurately analyzed by mathematical method, which has important guiding significance for the choice of control strategy and experiments and also has reference value for the later development of AUVs.
Kuntai Capsule plus Hormone Therapy vs. Hormone Therapy Alone in Patients with Premature Ovarian Failure: A Systematic Review and Meta-Analysis
Authors: Liu Weiping , Nguyen Truong-Nam , Tran Thi Thu-Van , Zhou Shaohu
Published: June 2019

The aim of this study was to evaluate the efficacy and safety of Kuntai capsules (KTC) plus hormone therapy (HT) compared to HT alone for the treatment of premature ovarian failure (POF). Databases including PubMed, MEDLINE, Web of Science, China National Knowledge Infrastructure (CNKI), the Chinese BioMedical database (CBM), and the Wanfang database were searched up to October 2018 for randomized controlled trials (RCTs). After screening the studies, extracting the data, and assessing the study quality, Cochrane RevMan 5.3 software was used to conduct a meta-analysis. Twelve RCTs involving 1178 patients were included. Regarding the therapeutic effects, total effective treatment rate was higher for the KTC+HT groups compared to the HT-only groups. Furthermore, compared with HT, KTC+HR effectively altered endocrine indexes involving serum levels of luteinizing hormone (weighted mean difference [WMD]=-3.47, 95% CI [5.68, -1.26], P=0.002]), follicle-stimulating hormone [WMD=-8.15, 95% CI [-10.44, -5.86], P<0.00001], estrogen [WMD=17.21, 95% CI [10.16, 24.26], P<0.00001], and anti-Müllerian hormone [WMD=1.07, 95% CI [0.78, 1.36], P<0.00001]; blood lipid indexes involving serum levels of triglyceride (WMD=-0.55, 95% CI [-0.76, -0.43], P<0.00001), total cholesterol (WMD=-0.63, 95% CI [-0.74, -0.52], P<0.00001), and low-density lipoprotein cholesterol (WMD=-0.62, 95% CI [-0.75, -0.49], P<0.00001); and B-ultrasound results involving ovarian resistance index (WMD=-0.20, 95% CI [-0.35, -0.04], P=0.01), perfusion index (WMD=-0.41, 95% CI [-0.57, -0.24], P<0.00001), peak systolic velocity (WMD=2.43, 95% CI [1.52, 3.34], P<0.00001), antral follicle count (WMD=1.20, 95% CI [0.41, 2.00], P=0.003), and mean ovarian diameter in the plane containing the longest axis of the ovary (WMD=4.34, 95% CI [2.94, 5.74], P<0.00001). There were no serious adverse events in either group. There is evidence that KTC+HT is more effective and safer than HT alone for treating POF. However, the trials had low methodological quality and small samples, so further standardized research is required.
The Effect of Ginkgo Biloba Dropping Pills on Hemorheology and Blood Lipid: A Systematic Review of Randomized Trials
Authors: Chen Hong , Zhou Cihang , Yu Mingwei , Feng Shuo , Ma Yunfei , Liu Zhengrong , Zhang Jiahui , Ding Tongjing , Li Bo , Wang Xiaomin
Published: June 2019

Objective. A systematic review of randomized trials was performed to assess the effect of Ginkgo Biloba Dropping Pills (GBDP) on clinical hemorheology and blood lipid indicators. Methods. The data of the Embase, Cochrane Library, PubMed, Clinical Trials, China National Knowledge Infrastructure, the Wanfang database, the VIP database, and the Sinomed were retrieved by computers from the establishment of the database to March 27, 2018, and screened and extracted by two researchers according to inclusion and exclusion criteria. Cochrane 5.0 recommended bias risk assessment tool was used to evaluate the methodological quality of the included literature, and Revman 5.3 software were used for meta-analysis. Results. 10 literatures were finally selected in accordance with the standard. There were a total of 1201 cases, 608 cases in ginkgo biloba dropping pill group and 593 in routine treatment group. Compared with control group, GBDP significantly improved plasma viscosity [N=383, RR= - 0.45, 95%CI=(-0.86,-0.04), P=0.03], whole blood high shear [N=232, RR= - 0.92,95%CI=(-1.69, -0.16), P =0.02], whole blood low shear [N = 232, RR = - 2.22, 95% CI = (- 3.74, -0.7), P = 0.004], red blood cell specific volume [N =132, RR = - 4.55, 95% CI = (- 6.36, 2.73), P < 0.000 01], fibrinogen [N=243, RR=-0.60,95%CI=(-0.82,-0.39), P<0.00001], triglyceride [N=912, RR=-0.60,95%CI=(-1.12, -0.07), P =0.03], cholesterol [N=912, RR=-0.97,95%CI=(-1.41, -0.52), P <0.0001], low-density cholesterol [N=1100, RR=-0.72,95%CI=(-1.19, -0.25), P =0.003], and sensitivity analysis before and after of high-density cholesterol [N=1020, RR=0.08,95%CI=(-0.17,0.34), P =0.52] and [N=683, RR=0.27,95%CI=(0.13,0.42), P =0.0003]. And seven adverse reactions were reported. Conclusion. GBDP can improve hemorheology indexes, which is to reduce the blood viscosity, to improve blood lipid status, and to prevent and treat cardiocerebral and renal vascular diseases to a certain extent, with slight clinical adverse reactions. But our results were based on small amount of clinical studies with poor quality and insufficient evidence, which may lead to low credibility of conclusions. Therefore, more large-sample, multiple-center, randomized controlled clinical trials and related mechanisms researches are needed to obtain better clinical trial evidence in order to verify the further effectiveness and safety of GBDP on hemorheology.
GAAP: A Genome Assembly + Annotation Pipeline
Authors: Kong Jinhwa , Huh Sun , Won Jung-Im , Yoon Jeehee , Kim Baeksop , Kim Kiyong
Published: June 2019

Genomic analysis begins with de novo assembly of short-read fragments in order to reconstruct full-length base sequences without exploiting a reference genome sequence. Then, in the annotation step, gene locations are identified within the base sequences, and the structures and functions of these genes are determined. Recently, a wide range of powerful tools have been developed and published for whole-genome analysis, enabling even individual researchers in small laboratories to perform whole-genome analyses on their objects of interest. However, these analytical tools are generally complex and use diverse algorithms, parameter setting methods, and input formats; thus, it remains difficult for individual researchers to select, utilize, and combine these tools to obtain their final results. To resolve these issues, we have developed a genome analysis pipeline (GAAP) for semiautomated, iterative, and high-throughput analysis of whole-genome data. This pipeline is designed to perform read correction, de novo genome (transcriptome) assembly, gene prediction, and functional annotation using a range of proven tools and databases. We aim to assist non-IT researchers by describing each stage of analysis in detail and discussing current approaches. We also provide practical advice on how to access and use the bioinformatics tools and databases and how to implement the provided suggestions. Whole-genome analysis of Toxocara canis is used as case study to show intermediate results at each stage, demonstrating the practicality of the proposed method.