bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Scale Audio Spectrogram Transformer for Classroom Teaching Interaction Recognition
Authors: Liu Fan , Fang Jiandong
Published: February 2023

Classroom interactivity is one of the important metrics for assessing classrooms, and identifying classroom interactivity through classroom image data is limited by the interference of complex teaching scenarios. However, audio data within the classroom are characterized by significant student–teacher interaction. This study proposes a multi-scale audio spectrogram transformer (MAST) speech scene classification algorithm and constructs a classroom interactive audio dataset to achieve interactive teacher–student recognition in the classroom teaching process. First, the original speech signal is sampled and pre-processed to generate a multi-channel spectrogram, which enhances the representation of features compared with single-channel features; Second, in order to efficiently capture the long-range global context of the audio spectrogram, the audio features are globally modeled by the multi-head self-attention mechanism of MAST, and the feature resolution is reduced during feature extraction to continuously enrich the layer-level features while reducing the model complexity; Finally, a further combination with a time-frequency enrichment module maps the final output to a class feature map, enabling accurate audio category recognition. The experimental comparison of MAST is carried out on the public environment audio dataset and the self-built classroom audio interaction datasets. Compared with the previous state-of-the-art methods on public datasets AudioSet and ESC-50, its accuracy has been improved by 3% and 5%, respectively, and the accuracy of the self-built classroom audio interaction dataset has reached 92.1%. These results demonstrate the effectiveness of MAST in the field of general audio classification and the smart classroom domain.
Evaluation of the Mechanical Properties of Lightweight Foamed Concrete at Varying Elevated Temperatures
Authors: Othuman Mydin Md Azree
Published: February 2023

Lightweight foamed concrete (LFC) made from cementitious materials with air pores entrapped in the matrix by mechanically entrained foam in the mortar slurry has several economic and environmental benefits. Most recently, LFC has been heralded as the next generation of lightweight construction industry concrete because of its versatility and technological advancements. Owing to its many desirable qualities, including low density, low cost, low thermal conductivity, low dimensional change, amazing load-bearing capacity, great workability, and low weight, it is considered an adaptable and flexible construction material. Given that LFC is a brittle building material and since fire is among the most frequent catastrophes to affect structures, it is crucial to consider the structural performance of LFC subjected to high temperatures. Hence, this experiment attempts to ascertain the effect of varying elevated temperatures on the LFC’s strength properties. Three LFC densities of 500, 1000 and 1500 kg/m3 were prepared. The LFC specimens were exposed to predetermined ambient and elevated temperatures of 20, 100, 200, 300, 400, 500, 600, 700 and 800 °C, and the LFC samples were assessed for porosity, compressive and flexural strengths. The outcomes of this investigation showed that, regardless of density, the loss of LFC stiffness exposed to elevated temperatures happened primarily after 90 °C. This shows that the underlying process triggering stiffness loss is internal cracking, that transpires when water grows and dissolves from a porous medium. Lowering the LFC dry density diminishes its strength and rigidity. The LFC-normalized strength and stiffness–temperature relationships of various dry densities, on the other hand, are very comparable. From ambient temperature up until 400 °C, all densities exhibit a moderate and constant loss in strength and stiffness. Nevertheless, the decline is faster up to 600 °C or 800 °C, at which point it loses its ability to support any given weight. This study emphasized the necessity for more study and codes’ provisions that take into consideration various LFC constituent types and cutting-edge construction material technologies.
Implementation of Chemometrics and Other Techniques as Means of Authenticity and Traceability to Detect Adulteration in Foods for the Protection of Human Health
Authors: Varzakas Theodoros
Published: February 2023

The authenticity of foods of plant and animal origin is key to safeguarding both quality and safety aspects without jeopardizing consumers’ health [...]
Development of a Halochromic, Antimicrobial, and Antioxidant Starch-Based Film Containing Phenolic Extract from Jaboticaba Peel
Authors: Luz Rafaela F. , Ferreira Richard D. R. , Silva Cassio N. S. , Miranda Bruna M. , Piccoli Roberta H. , Silva Monique S. , Paula Ladyslene C. , Leles Maria Inês G. , Fernandes Kátia F. , Cruz Maurício V. , Batista Karla A.
Published: February 2023

In this study, the antioxidant, antimicrobial, mechanical, optical, and barrier attributes of Solanum lycocarpum starch bio-based edible films incorporated with a phenolic extract from jaboticaba peel were investigated. Aiming to determine the effect of the polymers and the phenolic extract on the properties of the films, a three-factor simplex-lattice design was employed, and the formulation optimization was based on the produced films’ antioxidant potential. The optimized formulation of the starch-PEJP film showed a reddish-pink color with no cracks or bubbles and 91% antioxidant activity against DPPH radical. The optimized starch-PEJP film showed good transparency properties and a potent UV-blocking action, presenting color variation as a function of the pH values. The optimized film was also considerably resistant and highly flexible, showing a water vapor permeability of 3.28 × 10−6 g m−1 h−1 Pa−1. The microbial permeation test and antimicrobial evaluation demonstrated that the optimized starch-PEJP film avoided microbial contamination and was potent in reducing the growth of Escherichia coli, Staphylococcus aureus, and Salmonella spp. In summary, the active starch-PEJP film showed great potential as an environmentally friendly and halochromic material, presenting antioxidant and antimicrobial properties and high UV-protecting activity.
Evaluation of Immune Modulation by β-1,3; 1,6 D-Glucan Derived from Ganoderma lucidum in Healthy Adult Volunteers, A Randomized Controlled Trial
Authors: Chen Shiu-Nan , Nan Fan-Hua , Liu Ming-Wei , Yang Min-Feng , Chang Ya-Chih , Chen Sherwin
Published: February 2023

Fungi-derived β-glucan, a type of glucopolysaccharide, has been shown to possess immune-modulatory properties in clinical settings. Studies have indicated that β-glucan derived from Ganoderma lucidum (commonly known as Reishi) holds particular promise in this regard, both in laboratory and in vivo settings. To further investigate the efficacy and safety of Reishi β-glucan in human subjects, a randomized, double-blinded, placebo-controlled clinical trial was conducted among healthy adult volunteers aged 18 to 55. Participants were instructed to self-administer the interventions or placebos on a daily basis for 84 days, with bloodwork assessments conducted at the beginning and end of the study. The results of the trial showed that subjects in the intervention group, who received Reishi β-glucan, exhibited a significant enhancement in various immune cell populations, including CD3+, CD4+, CD8+ T-lymphocytes, as well as an improvement in the CD4/CD8 ratio and natural killer cell counts when compared to the placebo group. Additionally, a statistically significant difference was observed in serum immunoglobulin A levels and natural killer cell cytotoxicity between the intervention and placebo groups. Notably, the intervention was found to be safe and well tolerated, with no statistically significant changes observed in markers of kidney or liver function in either group. Overall, the study provides evidence for the ability of Reishi β-glucan to modulate immune responses in healthy adults, thereby potentially bolstering their defense against opportunistic infections.
Identification and Molecular Binding Mechanism of Novel α-Glucosidase Inhibitory Peptides from Hot-Pressed Peanut Meal Protein Hydrolysates
Authors: Yang Xinyu , Wang Dan , Dai Yangyong , Zhao Luping , Wang Wentao , Ding Xiuzhen
Published: February 2023

Hot-pressed peanut meal protein hydrolysates are rich in Arg residue, but there is a lack of research on their α-glucosidase inhibitory activity. In this study, different proteases were used to produce hot-pressed peanut meal protein hydrolysates (PMHs) to evaluate the α-glucosidase inhibitory activity. All PMHs showed good α-glucosidase inhibitory activity with the best inhibition effect coming from the dual enzyme system of Alcalase and Neutrase with an IC50 of 5.63 ± 0.19 mg/mL. The fractions with the highest inhibition effect were separated and purified using ultrafiltration and cation exchange chromatography. Four novel α-glucosidase inhibitory peptides (FYNPAAGR, PGVLPVAS, FFVPPSQQ, and FSYNPQAG) were identified by nano-HPLC-MS/MS and molecular docking. Molecular docking showed that peptides could occupy the active pocket of α-glucosidase through hydrogen bonding, hydrophobic interaction, salt bridges, and π-stacking, thus preventing the formation of complexes between α-glucosidase and the substrate. In addition, the α-glucosidase inhibitory activity of PMHs was stable against hot, pH treatment and in vitro gastrointestinal digestion. The study demonstrated that PMHs might be used as a natural anti-diabetic material with the potential to inhibit α-glucosidase.
Influence of Ultrasound-Assisted Vacuum Drying on Physicochemical Characteristics, Antioxidant Activity, and α-Glucosidase Inhibition Activity of Flos Sophorae Immaturus
Authors: Gong Yuhong , Li Jun , Li Jinwei , Fan Liuping , Wang Li
Published: February 2023

Flos Sophorae Immaturus (FSI) contains a large number of bioactive substances with antioxidant and hypoglycaemic activity. However, a feasible drying process plays an important role in the retention of its biological activity. The present work investigated the effects of ultrasound-assisted vacuum drying (UAVD) on FSI samples in terms of drying time, colour, microstructure, and total flavonoid content (TFC). Meanwhile, the antioxidant activity and α-glucosidase inhibition activity were also evaluated. The results show that the drying time of UVAD samples was decreased by 40% compared to that of the single vacuum-dried (VD) samples (600 W for 10 min). The cellular porous structures of FSI tissue were formed by UAVD, which promoted the migration of water from the inside to the outside. Furthermore, samples treated by UAVD exhibited better antioxidant activities and α-glucosidase and α-amylase inhibition capacities, with DPPH (81.86%), ABTS (88.61%), FRAP (83.05%), α-glucosidase inhibition capacity (89%), α-amylase (85%), drying time (3 h), and total aberration (ΔE) (1.63) being the highest characteristic traits. In this condition, the highest levels of total flavonoid content (TFC), rutin, quercetin, kaempferol, isorhamnetin, and genistein were obtained with 266.94, 239.46, 35.56, 8.54, 10.37, and 5.64 mg/g DW, respectively. The results confirm that UAVD is a novel method that significantly reduced the VD time and promoted the release of the bioactive substances of FSI.
Analysis of a Fractional Variational Problem Associated with Cantilever Beams Subjected to a Uniformly Distributed Load
Authors: Suechoei Apassara , Sa Ngiamsunthorn Parinya , Chatanin Waraporn , Athisakul Chainarong , Chucheepsakul Somchai , Songsanga Danuruj
Published: February 2023

In this paper, we investigate the existence and uniqueness of minimizers of a fractional variational problem generalized from the energy functional associated with a cantilever beam under a uniformly distributed load. We apply the fractional Euler–Lagrange condition to formulate the minimization problem as a boundary value problem and obtain existence and uniqueness results in both L2 and L∞ settings. Additionally, we characterize the continuous dependence of the minimizers on varying loads in the energy functional. Moreover, an approximate solution is derived via the homotopy perturbation method, which is numerically demonstrated in various examples. The results show that the deformations are larger for smaller orders of the fractional derivative.
RBF-Based Local Meshless Method for Fractional Diffusion Equations
Authors: Kamran Kamran , Irfan Muhammad , Alotaibi Fahad M. , Haque Salma , Mlaiki Nabil , Shah Kamal
Published: February 2023

The fractional diffusion equation is one of the important recent models that can efficiently characterize various complex diffusion processes, such as in inhomogeneous or heterogeneous media or in porous media. This article provides a method for the numerical simulation of time-fractional diffusion equations. The proposed scheme combines the local meshless method based on a radial basis function (RBF) with Laplace transform. This scheme first implements the Laplace transform to reduce the given problem to a time-independent inhomogeneous problem in the Laplace domain, and then the RBF-based local meshless method is utilized to obtain the solution of the reduced problem in the Laplace domain. Finally, Stehfest’s method is utilized to convert the solution from the Laplace domain into the real domain. The proposed method uses Laplace transform to handle the fractional order derivative, which avoids the computation of a convolution integral in a fractional order derivative and overcomes the effect of time-stepping on stability and accuracy. The method is tested using four numerical examples. All the results demonstrate that the proposed method is easy to implement, accurate, efficient and has low computational costs.
Determination of Three-Dimensional Brinkman—Forchheimer-Extended Darcy Flow
Authors: Tao Zhengwang , Yang Xin-Guang , Lin Yan , Guo Chunxiao
Published: February 2023

The aim of this study is to determine a 3D incompressible Brinkman–Forchheimer-extended Darcy fluid flow. Based on global well-posedness and regularity of solutions with a periodic boundary condition, the determining modes for weak and regular solutions is achieved via the generalized Grashof number for a 3D non-autonomous Brinkman–Forchheimer-Darcy fluid flow in porous medium. Furthermore, the asymptotic determination of the complete trajectories inside an attractor via Fourier functionals is shown for a 3D autonomous Brinkman–Forchheimer-extended Darcy model.
The Hydrogen Color Spectrum: Techno-Economic Analysis of the Available Technologies for Hydrogen Production
Authors: Arcos Jose M. Marín , Santos Diogo M. F.
Published: February 2023

Hydrogen has become the most promising energy carrier for the future. The spotlight is now on green hydrogen, produced with water electrolysis powered exclusively by renewable energy sources. However, several other technologies and sources are available or under development to satisfy the current and future hydrogen demand. In fact, hydrogen production involves different resources and energy loads, depending on the production method used. Therefore, the industry has tried to set a classification code for this energy carrier. This is done by using colors that reflect the hydrogen production method, the resources consumed to produce the required energy, and the number of emissions generated during the process. Depending on the reviewed literature, some colors have slightly different definitions, thus making the classifications imprecise. Therefore, this techno-economic analysis clarifies the meaning of each hydrogen color by systematically reviewing their production methods, consumed energy sources, and generated emissions. Then, an economic assessment compares the costs of the various hydrogen colors and examines the most feasible ones and their potential evolution. The scientific community and industry’s clear understanding of the advantages and drawbacks of each element of the hydrogen color spectrum is an essential step toward reaching a sustainable hydrogen economy.
Efficacy of 5% Aminolaevulinic Acid and Red Light on Enterococcus faecalis in Infected Root Canals
Authors: Carlesi Teocrito , Dotta Tatiane Cristina , Pierfelice Tania Vanessa , D’Amico Emira , Lepore Stefania , Tripodi Domenico , Piattelli Adriano , D’Ercole Simonetta , Petrini Morena
Published: February 2023

Background: In this ex vivo study, the aim was to evaluate the effects of ALAD and red light on Enterococcus faecalis in infected root canals using a special intracanal fiber. Methods: A total of 70 extracted, single-rooted teeth were used. The teeth were decoronated at the length of the roots to approximately 15 mm and then instrumented. The apical foramen was sealed by composite resin, and the root canals were infected with a pure culture of E. faecalis ATCC 29212 for eight days at 37 °C. Following the contamination period, the roots were divided into seven groups, including the positive and negative control groups, and treated as follows: ALAD 45 min; red light activation 7 min; ALAD 45 min and red-light activation 7 min; sodium hypochlorite 2.5% 15 min; sodium hypochlorite 1% 15 min. The samples were taken by three sterile paper points, transferred to tubes containing 1 mL of PBS, and immediately processed for the number of colony-forming units and the cell viability by using live/dead. Results: The best treatment is obtained with 2.5% NaOCl. Except for ALAD + red light vs. 1% NaOCl, a statistically significant difference is recorded for all treatments. The combination of 2.5% NaOCl and ALAD + 7 min irradiation produces an evident killing effect on the E. faecalis cells. On the other hand, 1% NaOCl is ineffective for the viability action, with 25% of dead cells stained in red. Conclusions: This ex vivo study shows that ALAD gel with light irradiation is an efficacious protocol that exerts a potent antibacterial activity against E. faecalis in infected root canals.
A New Design of Poly(N-Isopropylacrylamide) Hydrogels Using Biodegradable Poly(Beta-Aminoester) Crosslinkers as Fertilizer Reservoirs for Agricultural Applications
Authors: Balçık Tamer Yasemin
Published: February 2023

Poly(N-isopropylacrylamide) (P(NIPAAm)) hydrogels were prepared by free-radical polymerization with biodegradable poly (β-amino ester) (PBAE) crosslinkers at 1 wt% and 3 wt% ratio, and compared with conventional N,N′-methylene bisacrylamide (MBA)-crosslinked hydrogel. The influence of the type, molecular weight, and diacrylate/amine ratio of the crosslinker on the crosslink density, compressive strength, and swelling and biodegradation behavior of the hydrogels was investigated. The hydrogels synthesized with lower molecular weight PBAE crosslinkers showed higher crosslinking degrees and compressive strength and lower swelling ratios. To reveal the controlled release behavior of the fertilizer, KNO3 was used as the model, and its loading and release behavior from these hydrogels was also examined. The N/T5/1 sample with 1.5/1.0 diacrylate/amine molar ratio and 1 wt% PBAE ratio demonstrated the most controlled release of KNO3 with 66.9% after 18 days in soil. In addition, the hydrogel with the porosity of 71.65% and crosslinking degree of 2.85 × 10−5 mol cm−3 showed a swelling ratio of 69.44 g/g, biodegradation rate of 23.9%, and compressive strength of 1.074 MPa. Thus, it can be concluded that the new designed biodegradable P(NIPAAm) hydrogels can be promising materials as nitrate fertilizer reservoirs and also for controlled fertilizer release in soil media for agricultural applications.
Integrating Parental Phenotypic Data Enhances Prediction Accuracy of Hybrids in Wheat Traits
Authors: Montesinos-López Osval A. , Bentley Alison R. , Saint Pierre Carolina , Crespo-Herrera Leonardo , Salinas Ruiz Josafhat , Valladares-Celis Patricia Edwigis , Montesinos-López Abelardo , Crossa José
Published: February 2023

Genomic selection (GS) is a methodology that is revolutionizing plant breeding because it can select candidate genotypes without phenotypic evaluation in the field. However, its practical implementation in hybrid prediction remains challenging since many factors affect its accuracy. The main objective of this study was to research the genomic prediction accuracy of wheat hybrids by adding covariates with the hybrid parental phenotypic information to the model. Four types of different models (MA, MB, MC, and MD) with one covariate (same trait to be predicted) (MA_C, MB_C, MC_C, and MD_C) or several covariates (of the same trait and other correlated traits) (MA_AC, MB_AC, MC_AC, and MD_AC) were studied. We found that the four models with parental information outperformed models without parental information in terms of mean square error by at least 14.1% (MA vs. MA_C), 5.5% (MB vs. MB_C), 51.4% (MC vs. MC_C), and 6.4% (MD vs. MD_C) when parental information of the same trait was used and by at least 13.7% (MA vs. MA_AC), 5.3% (MB vs. MB_AC), 55.1% (MC vs. MC_AC), and 6.0% (MD vs. MD_AC) when parental information of the same trait and other correlated traits were used. Our results also show a large gain in prediction accuracy when covariates were considered using the parental phenotypic information, as opposed to marker information. Finally, our results empirically demonstrate that a significant improvement in prediction accuracy was gained by adding parental phenotypic information as covariates; however, this is expensive since, in many breeding programs, the parental phenotypic information is unavailable.
Global Distribution of Founder Variants Associated with Non-Syndromic Hearing Impairment
Authors: Aboagye Elvis Twumasi , Adadey Samuel Mawuli , Wonkam-Tingang Edmond , Amenga-Etego Lucas , Awandare Gordon A. , Wonkam Ambroise
Published: February 2023

The genetic etiology of non-syndromic hearing impairment (NSHI) is highly heterogeneous with over 124 distinct genes identified. The wide spectrum of implicated genes has challenged the implementation of molecular diagnosis with equal clinical validity in all settings. Differential frequencies of allelic variants in the most common NSHI causal gene, gap junction beta 2 (GJB2), has been described as stemming from the segregation of a founder variant and/or spontaneous germline variant hot spots. We aimed to systematically review the global distribution and provenance of founder variants associated with NSHI. The study protocol was registered on PROSPERO, the International Prospective Register of Systematic Reviews, with the registration number “CRD42020198573”. Data from 52 reports, involving 27,959 study participants from 24 countries, reporting 56 founder pathogenic or likely pathogenic (P/LP) variants in 14 genes (GJB2, GJB6, GSDME, TMC1, TMIE, TMPRSS3, KCNQ4, PJVK, OTOF, EYA4, MYO15A, PDZD7, CLDN14, and CDH23), were reviewed. Varied number short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs) were used for haplotype analysis to identify the shared ancestral informative markers in a linkage disequilibrium and variants’ origins, age estimates, and common ancestry computations in the reviewed reports. Asia recorded the highest number of NSHI founder variants (85.7%; 48/56), with variants in all 14 genes, followed by Europe (16.1%; 9/56). GJB2 had the highest number of ethnic-specific P/LP founder variants. This review reports on the global distribution of NSHI founder variants and relates their evolution to population migration history, bottleneck events, and demographic changes in populations linked with the early evolution of deleterious founder alleles. International migration and regional and cultural intermarriage, coupled to rapid population growth, may have contributed to re-shaping the genetic architecture and structural dynamics of populations segregating these pathogenic founder variants. We have highlighted and showed the paucity of data on hearing impairment (HI) variants in Africa, establishing unexplored opportunities in genetic traits.
CD99 and the Chicken Alloantigen D Blood System
Authors: Fulton Janet E. , Drobik-Czwarno Wiola , Lund Ashlee R. , Schmidt Carl J. , Taylor Robert L.
Published: February 2023

The chicken D blood system is one of 13 alloantigen systems found on chicken red blood cells. Classical recombinant studies located the D blood system on chicken chromosome 1, but the candidate gene was unknown. Multiple resources were utilized to identify the chicken D system candidate gene, including genome sequence information from both research and elite egg production lines for which D system alloantigen alleles were reported, and DNA from both pedigree and non-pedigree samples with known D alleles. Genome-wide association analyses using a 600 K or a 54 K SNP chip plus DNA from independent samples identified a strong peak on chicken chromosome 1 at 125–131 Mb (GRCg6a). Cell surface expression and the presence of exonic non-synonymous SNP were used to identify the candidate gene. The chicken CD99 gene showed the co-segregation of SNP-defined haplotypes and serologically defined D blood system alleles. The CD99 protein mediates multiple cellular processes including leukocyte migration, T-cell adhesion, and transmembrane protein transport, affecting peripheral immune responses. The corresponding human gene is found syntenic to the pseudoautosomal region 1 of human X and Y chromosomes. Phylogenetic analyses show that CD99 has a paralog, XG, that arose by duplication in the last common ancestor of the amniotes.
Pharmacological Support for the Treatment of Obesity—Present and Future
Authors: Kosmalski Marcin , Deska Kacper , Bąk Bartłomiej , Różycka-Kosmalska Monika , Pietras Tadeusz
Published: February 2023

Obesity is a growing civilization problem, associated with a number of negative health consequences affecting almost all tissues and organs. Currently, obesity treatment includes lifestyle modifications (including diet and exercise), pharmacologic therapies, and in some clinical situations, bariatric surgery. These treatments seem to be the most effective method supporting the treatment of obesity. However, they are many limitations to the options, both for the practitioners and patients. Often the comorbidities, cost, age of the patient, and even geographic locations may influence the choices. The pharmacotherapy of obesity is a fast-growing market. Currently, we have at our disposal drugs with various mechanisms of action (directly reducing the absorption of calories—orlistat, acting centrally—bupropion with naltrexone, phentermine with topiramate, or multidirectional—liraglutide, dulaglutide, semaglutide). The drugs whose weight-reducing effect is used in the course of the pharmacotherapy of other diseases (e.g., glucose-sodium cotransporter inhibitors, exenatide) are also worth mentioning. The obesity pharmacotherapy is focusing on novel therapeutic agents with improved safety and efficacy profiles. These trends also include an assessment of the usefulness of the weight-reducing properties of the drugs previously used for other diseases. The presented paper is an overview of the studies related to both drugs currently used in the pharmacotherapy of obesity and those undergoing clinical trials, taking into account the individual approach to the patient.
Synergistic Interactions between the Hypomethylating Agent Thio-Deoxycytidine and Venetoclax in Myelodysplastic Syndrome Cells
Authors: Hu Xiaoyan , Li Lin , Nkwocha Jewel , Sharma Kanika , Zhou Liang , Grant Steven
Published: February 2023

Interactions between the novel hypomethylating agent (HMA) thio-deoxycytidine (T-dCyd) and the BCL-2 antagonist ABT-199 (venetoclax) have been examined in human myelodysplastic syndrome (MDS) cells. The cells were exposed to agents alone or in combination, after which apoptosis was assessed, and a Western blot analysis was performed. Co-administration of T-dCyd and ABT-199 was associated with the down-regulation of DNA methyltransferase 1 (DNMT1) and synergistic interactions documented by a Median Dose Effect analysis in multiple MDS-derived lines (e.g., MOLM-13, SKM-1, and F-36P). Inducible BCL-2 knock-down significantly increased T-dCyd’s lethality in MOLM-13 cells. Similar interactions were observed in the primary MDS cells, but not in the normal cord blood CD34+ cells. Enhanced killing by the T-dCyd/ABT-199 regimen was associated with increased reactive oxygen species (ROS) generation and the down-regulation of the anti-oxidant proteins Nrf2 and HO-1, as well as BCL-2. Moreover, ROS scavengers (e.g., NAC) reduced lethality. Collectively, these data suggest that combining T-dCyd with ABT-199 kills MDS cells through an ROS-dependent mechanism, and we argue that this strategy warrants consideration in MDS therapy.
Genome-Wide Analysis of the AP2/ERF Family in Oily Persimmon (Diospyros oleifera) and Their Preliminary Roles Exploration in Response to Polyamines for Adventitious Root Formation in Cultivated Persimmon (D. kaki)
Authors: Wang Yanbo , Du Xiaoyun , Liu Minxiao , Liu Xueqing , Zhao Lingling , Cao Luyao , Zhang Shuo , Song Laiqing , Sun Yanxia , Liu Daliang , Yu Xiaoli , Xu Junchi
Published: February 2023

The AP2/ERF (APETALA2/Ethylene−Responsive element binding factor) family genes play crucial roles in plant growth and development, and responses to environmental factors; however, this family has not been characterized in Diospyros species. In Diospyros, the diploid Oily persimmon (D. oleifera, 2n = 2x = 30) has been released with complete genome assembly, which makes it possible for genome-wide gene family identification and exploration of molecular function in cultivated persimmon (D. kaki, 2n = 6x = 90). Here, we identified the AP2/ERF family in Oily persimmon for the first time and investigated its classification, main physicochemical properties, structural characteristic, chromosome distribution, gene replication and collinearity, cis-factor binding sites deduction, GO term annotation, and PPI interaction, as well as its expression profiles in different tissue and under the treatment of polyamines. A total of 157 AP2/ERF genes, including four subfamilies (AP2, RAV, Soloist, and ERF), were identified with distribution on all 15 chromosomes. DkAP2/ERF gene expression patterns were extensive and diverse. They were detected expression in every examined tissue, with the highest number of DkAP2/ERF genes expressed in the root. DkAP2/ERF gene expression analysis in adventitious root generation and elongation of polyamines showed their different responses to the action of polyamines, and more pairs of DkAP2/ERF genes with high correlation in gene expression were obtained. In addition, some DkAP2/ERF genes were detected remarkably correlated with genes related to polyamine synthesis and cell metabolism, including S-adenosyl-L-methionine Decarboxyla2 (SAMDC2), D-type cyclin1 (CYCD1), and D-type cyclin2 (CYCD2) genes,. indicating that DkAP2/ERF genes may play a synergistic role in adventitious root development This study was the first to analyze the AP2/ERF gene comprehensively in Diospyros on a genome−wide scale and will provide insights into the application of adventitious root formation in cultivated persimmon.
Effects of Gamma Amino Butyric Acid (GABA) on Nutrient Uptake of Loquat [Eriobotrya japonica (Thunb.) Lindl.] Seedlings
Authors: Yang Zhiwu , Xu Yaxin , Song Panhui , Li Xinyu , Zhou Jiayun , Lin Lijin , Xia Hui , Liang Dong , Luo Xian , Zhang Huifen , Deng Qunxian , Wang Yongqing
Published: February 2023

Gamma amino butyric acid (GABA), an important free amino acid in plant tissues, plays an essential role in all stages of plant growth and development. In this study, we aimed to explore the effects of GABA on the nutrient absorption of loquat [Eriobotrya japonica (Thunb.) Lindl.] seedlings. The effects of applying exogenous GABA in different concentrations (0.0, 0.5, 1.0, 1.5, and 2 g L−1) on the nutrient uptake of loquat seedlings were studied. GABA increased the biomass (dry weight) and contents of photosynthetic pigments in loquat seedlings to a certain extent. GABA concentration exhibited a quadratic polynomial regression relationship with the biomass. Exogenous GABA in different concentrations increased the total nitrogen (N) and phosphorus (P) contents in loquat seedlings, whereas only 0.5 and 1.0 g L−1 of GABA increased the potassium (K) content. Similarly, GABA concentration also had a polynomial regression relationship with the total N, P, and K contents. Compared to the control, 0.5, 1.0, 1.5, and 2.0 g L−1 of GABA increased the shoot total N content by 27.30, 32.99, 15.41, and 12.93%, respectively, and also increased the shoot total P content by 26.12, 37.52, 21.99, and 9.61%, respectively. Furthermore, correlation and grey relational analyses showed that the carotenoid content, root biomass, and soil alkali-hydrolyzable N concentration were the indicators most closely associated with the uptakes of N, P, and K in shoots. This study shows that exogenous GABA can promote the growth and nutrient uptake of loquat seedlings at an optimum concentration of 1.0 g L−1.
Photosynthesis Characteristics of Tomato Plants and Its’ Responses to Microclimate in New Solar Greenhouse in North China
Authors: Liu Haijun , Shao Mengxuan , Yang Li
Published: February 2023

With the increasing demand for vegetable fruits, vegetable plants are moved to protected structures for achieving high production and economic revenue, especially in undesirable seasons. In North China, tomato crops, as widely consumed vegetables, are now increasingly planted in solar greenhouses (GH), especially in the winter period. To improve the microclimate inside GH in winter, a sunken solar greenhouse was used recently. This study was to evaluate the photosynthetic characteristics of tomato plants and its responses to the inside microclimate in this new GH. In this experiment, the plant transpiration (E) and photosynthesis (Pn) rates of healthy and diseased plants were measured from July to December for three growth seasons in a commercial GH in North China. Results show both E and Pn were positively related to inside radiation and vapor pressure deficit. The stomata conductance to E (gsw) and Pn (gtc) performed relatively constant during daytime, and weakly related to inside microclimate. The parameters of E, Pn, gsw and gtc were greatly reduced for diseased plants in summer because of the heat shock. The water use efficiency at the leaf level, the ratio of Pn to E, was higher for solar radiation of 400–500 W m−2, temperature of 20–30 °C, relative humidity of higher than 80%, and vapor pressure deficit of less than 2.0 kPa. The results of this study could help farmers in the region of 30 to 40 degrees north latitude to enhance the growth of tomato crops in winter by using this sunken solar greenhouse.
Hydrolate and EO Application to Reduce Decay of Carica papaya during Storage
Authors: Culmone Alessandra , Mirabile Giulia , Tinebra Ilenia , Michelozzi Marco , Carrubba Alessandra , Bellardi Maria Grazia , Farina Vittorio , Romanazzi Gianfranco , Torta Livio
Published: February 2023

Postharvest fruit loss is caused by the absence of advanced handling and storage technologies and the quiescent presence of fungal pathogens. Therefore, there is a growing demand for sustainable decisions for the planet. This study focused on the use of two types of edible coatings: one was based on the essential oil of Origanum vulgare L. subsp. viridulum with Aloe arborescens Mill. gel (EC1), and the other was based on the hydrolate only (EC2). These treatments were applied to provide defense against fungal infections in papaya (Carica papaya L. cv Solo), and the storage time was 25 days (T5 ± 1 °C). Fruits coated with EC1 were more contaminated with fungal pathogens than both control (CTR) and EC2 fruit. EC2 showed a statistically lower decay index than CTR and EC1 and maintained its organoleptic characteristics better, showing a 15% loss of firmness after 25 days of storage. Furthermore, the lowest decay index (1.14 after 25 days) was found for the EC1 and CTR. These findings suggest that the use of hydrolate can be useful for extending the shelf life and maintaining the quality of papaya fruit, representing an alternative to the use of synthetic fungicides for food safety.
Drone Delivery of Dehydro-Sulfurization Utilizing Doubly-Charged Negative Ions of Nanoscale Catalysts Inspired by the Biomimicry of Bee Species’ Bio-Catalysis of Pollen Conversion to Organic Honey
Authors: Suggs Kelvin L. , Samarakoon Duminda K. , Msezane Alfred Z.
Published: February 2023

The sulfur dioxide (SO2) compound is a primary environmental pollutant worldwide, whereas elemental sulfur (S) is a global commodity possessing a variety of industrial as well as commercial functions. The chemical relationship between poisonous SO2 and commercially viable elemental S has motivated this investigation using the Density Functional Theory calculation of the relative transition state barriers for the two-step dehydro-sulfurization oxidation–reduction reaction. Additionally, doubly-charged nanoscale platelet molybdenum disulfide (MoS2), armchair (6,6) carbon nanotube, 28-atom graphene nanoflake (GR-28), and fullerene C-60 are utilized as catalysts. The optimal heterogeneous and homogeneous catalysis pathways of the two-step oxidation–reduction from SO2 to elemental S are further inspired by the biomimicry of the honeybee species’ multi-step bio-catalysis of pollen conversion to organic honey. Potential applications include environmental depollution, the mining of elemental sulfur, and the functionalization of novel technologies such as the recently patented aerial and amphibious LynchpinTM drones.
Effect of Mobile Phone Addiction on Physical Exercise in University Students: Moderating Effect of Peer Relationships
Authors: Han Yahui , Qin Guoyou , Han Shanshan , Ke Youzhi , Meng Shuqiao , Tong Wenxia , Guo Qiang , Li Yaxing , Ye Yupeng , Shi Wenya
Published: February 2023

Objective: The influence of mobile phone addiction (MPA) on physical exercise in university students was explored, and peer relationships were introduced as a moderating variable. Methods: A cross-sectional study design was adopted, and an online survey questionnaire was conducted to investigate two universities in Nantong City, Jiangsu Province, and Chongzuo City, Guangxi Zhuang Autonomous Region. A total of 4959 university students completed the questionnaire. Measurement tools included the Mobile Phone Addiction Tendency Scale, the Physical Activity Rating Scale, and the Peer Rating Scale of university students. Results: University students scored 39.322 ± 15.139 for MPA and 44.022 ± 7.735 for peer relationships, with 87.8% of their physical exercise, in terms of exercise grade, being classified as medium or low intensity. The MPA of the university students was negatively correlated with peer relationships (r = −0.377, p < 0.001) and physical exercise behavior (r = −0.279, p < 0.001). The moderating effect of peer relationships on the MPA-physical exercise behavior relationship was significant (ΔR2 = 0.03, p < 0.001). Conclusions: The physical exercise of university students was at a medium or low intensity. The more serious the university students’ addiction to mobile phones was, the lower the amount of physical exercise. The physical activity of males was higher than that of females. MPA and peer relationships were the limiting factors of the physical exercise behavior of university students. Under the lower effect of peer relationship regulation, MPA had a greater negative impact on physical exercise behavior. The data from this research can provide theoretical support to improve the participation of university students in physical activities.
Assessment of Radiation Risk Perception and Interest in Tritiated Water among Returnees to and Evacuees from Tomioka Town within 20 km of the Fukushima Daiichi Nuclear Power Plant
Authors: Xiao Xu , Matsunaga Hitomi , Orita Makiko , Kashiwazaki Yuya , Taira Yasuyuki , Win Thu Zar , Lochard Jacques , Schneider Thierry , Takamura Noboru
Published: February 2023

In this study, we aimed to investigate radiation risk perception, mental health, and interest in tritiated water among evacuees from and returnees to Tomioka town, Japan, as well as to evaluate the intention to return (ITR) among evacuees living inside and outside Fukushima Prefecture. Of the 1728 respondents, 318 (18.4%) and 1203 (69.6%) participants reported living outside and inside Fukushima Prefecture, and 207 (12.0%) reported living in Tomioka. The ITR was not significantly different between those who lived inside and outside the prefecture among the evacuees. Similarly, there were no significant differences in radiation risk perception, mental health, and interest in tritiated water. However, the evacuees were independently associated with a motivation to learn about tritiated water (OR = 1.242, 95%Cl: 1.041–1.438, p = 0.016), reluctance to consume food from Tomioka (OR = 1.635, 95%Cl: 1.372–1.948, p < 0.001), and concern that adverse health effects would occur because of the Fukushima Daiichi Nuclear Power Plant accident (OR = 1.279, 95%Cl: 1.055–1.550, p = 0.012) compared to returnees, according to logistic regression analysis. Interestingly, the returnees were found to have better mental health but lower life satisfaction than the evacuees. These findings suggest the importance of ongoing risk communication about radiation exposure and tritiated water among residents regardless of their place of residency.
Appropriateness of the Prescription and Use of Medicines: An Old Concept but More Relevant than Ever
Authors: Casula Manuela , Ardoino Ilaria , Franchi Carlotta
Published: February 2023

The availability of drugs to treat diseases, control symptoms, or prevent their onset is one of the most important resources for maintaining health [...]
Development of a Framework for On-Demand Caesarean Section in Romania
Authors: Petre Ion , Barna Flavia , Cîtu Cosmin , Gorun Florin , Gorun Oana-Maria , Tomescu Laurentiu Cezar , Apostol Adrian , Bordianu Anca , Furau Cristian , Petre Izabella
Published: February 2023

Background: Caesarean section rates have continued to trend upward in most countries, including Romania, creating a number of economic challenges. In the public health system, there is no regulation for performing Caesarean sections on demand; it is often done unlawfully, and in private hospitals, it is a real business. Thus, this study aims to investigate the budgetary impact at a hospital level and the profit per procedure by introducing on-demand caesarean sections for a fee. Methods: This study was conducted in one of the largest maternity units in Western Romania—the “Bega” Maternity Clinic of the Timisoara County Emergency Hospital. For the analysis, the difference between a proposed occupancy rate (between 50 and 85%, increasing every 5 percent) and the actual occupancy rate was calculated. Considering that this difference can be used to admit patients to receive Caesarean sections on demand for a fee, the profit that could be obtained during the study period was calculated. Results: It is reported that between 238 (proposed occupancy rate of 50%) and 4683 patients (a proposed occupancy rate of 85%) could have benefited from on-demand caesarean section surgery in 2017–2019. Between RON 419,999 and RON 8,551,636 could be obtained in the 3 years of study by implementing caesarean section against payment. Conclusion: The implementation of a system of on-demand payment for caesarean sections in Romania would bring significant profits to the hospital budget.
Current Situations and Challenges in the Development of Health Information Literacy
Authors: Wang Qiulin , Tao Chunhua , Yuan Yuan , Zhang Song , Liang Jingyan
Published: February 2023

Health information literacy (HIL) is a significant concept that has gradually become known to the broader public in recent years. Although the definitions of HIL and health literacy seem to overlap, as an independent subconcept, HIL still shows a unique influence on improvements in people’s health and health education. Remarkable evidence indicates that online health information (OHI) can effectively enrich people’s knowledge and encourage patients to actively join the medical process, which is also accompanied by the emergence of various assessment tools. Although the current assessment tools, to a certain extent, can help people identify their shortcomings and improve their HIL, many studies have indicated that the deficiencies of the scales induce incomplete or unreal results of their HIL. In addition, continuing research has revealed an increasing number of influencing factors that have great effects on HIL and even regulate the different trends in doctor–patient relationships. Simultaneously, most of the uncensored OHI broadcasts have also affected the improvement in HIL in various ways. Thus, this review aims to summarize the assessment tools, influencing factors and current situations and challenges related to HIL. Further studies are required to provide more trusted and deeper references for the development of HIL.
Symptoms of Sarcopenia and Physical Fitness through the Senior Fitness Test
Authors: Lima Alex Barreto de , Baptista Fátima , Henrinques-Neto Duarte , Pinto André de Araújo , Gouveia Elvio Rúbio
Published: February 2023

Introduction: Physical fitness concerns a set of attributes related to the ability to perform physical activity that may justify the symptoms reported by the elderly in the context of sarcopenia. Objective: This study aimed to investigate the relationship between the perception (symptomatology) of physical functioning (what the person thinks they are capable of) and the capacity itself for physical functioning in elderly people in northern Brazil. Methods: Cross-sectional study that analyzed 312 elderly people (72.6 ± 7.8 years) from the city of Novo Aripuanã, Amazonas, Brazil. Sarcopenia symptomatology was assessed using the SARC-F, a 5-item questionnaire designed for screening sarcopenia in older individuals in five domains: strength, walking aids, difficulty getting up from a chair, difficulty climbing stairs, and falls. Physical fitness was assessed by the Senior Fitness Test (SFT) battery including balance evaluated with the short version of the Fullerton Advanced Balance scale (FAB). Results: ROC curve analysis revealed that the tests with the greatest ability to discriminate participants with significant symptoms for sarcopenia (≥4 points on SARC-F) were arm curl and 6 min walk: the probability of suspected sarcopenia increased exponentially with an arm curl < 11.5 reps for men (se = 71%; sp = 69%; AUC = 0.706, 95% CI: 0.612–0.788; p = 0.013) and women (se = 81%; sp = 51%; AUC = 0.671, 95% CI: 0.601–0.735; p ≤ 0.001) or with a 6-min walk <408.5 m for men (se = 71%; sp = 63%; AUC = 0.720, 95% CI: 0.628–0.690; p = 0.001) and <366.0 m for women (se = 69%; sp = 58%; AUC = 0.692, 95% CI: 0.623–0.755; p = 0.0001). Conclusions: Physical fitness assessed through the senior fitness test, particularly the 30-s-arm curl test and the 6-min walk test, can discriminate for suspected symptoms of sarcopenia.