bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting the Immune Microenvironment and Prognosis with a NETosis‐Related lncRNA Signature in Head and Neck Squamous Cell Carcinoma
Authors: He Xiaohua , Xiao Yinglu , Liu Shan , Deng Ruyan , Li Zhiming , Zhu Xianying
Published: September 2022

Background. The mechanistic aspects of the involvement of long noncoding RNAs (lncRNAs) in NETosis, the process of neutrophil extracellular trap (NET) formation in head and neck squamous cell carcinoma (HNSCC), lack comprehensive elucidation. The involvement of these molecules in the immune microenvironment and plausible HNSCC prognosis remain to see the light of the day. The plausible functioning of NETosis‐related lncRNAs with their plausible prognostic impact in HNSCC was probed in this work. Methods. The scrutiny of lncRNAs linked to NETosis entailed the probing of twenty‐four genes associated with the process employing Pearson’s correlation analysis on HNSCC patients’ RNA sequencing data from The Cancer Genome Atlas (TCGA) database. The application of univariate, least absolute shrinkage and selection operator (LASSO), and multivariate Cox regression analyses yielded a NETosis‐related lncRNA signature that was subjected to probing for its suitability in prognosis employing survival and nomogram analyses. Results. The NETosis‐related lncRNA signature inclusive of five lncRNAs facilitated patients to be segregated as high‐risk and low‐risk groups with the former documenting a poor prognosis. Regression unearthed that the risk score was an independent factor for prognosis. The receiver operating characteristic (ROC) or receiver operating characteristic curve analysis documented a one‐year area under time‐dependent ROC curve (AUC) value of 0.711 that is corroborative of the accuracy of this signature. Additional probing documented an evident enriching of immune‐linked pathways in the low‐risk patients, while the high‐risk patients documented an immunologically “cold” profile as per the infiltration of immune cells. We verified lncRNA expression from our NETosis‐related lncRNA signature in vitro, which reflects the reliability of our model to a certain extent. Moreover, we also verified the function of the lncRNA. We found that LINC00426 contributes to the innate immune cGAS‐STING signaling pathway, which explain to some extent the role of our prognostic model in predicting “hot” and “cold” tumors. Conclusions. The plausible prognostic relevance of the NETosis‐related lncRNA signature (with five lncRNAs) emerges that is suggestive of its promise in targeting HNSCC.
[Retracted] Water Environment Governance of Urban and Rural Spaces Integrating Natural Ecological Landscape Design Method
Authors: Li Jun
Published: September 2022

The water environment is one of the basic elements that constitute the environment. It is an important place for the survival and development of human society, and it is also the most seriously disturbed and damaged area by humans. The pollution and destruction of water environment has become one of the major environmental problems in the world today. The essence of urban water space landscape design under the concept of integrating people’s ecological design is the ecological landscape design of urban water spaces, while the development of ecological landscape design in the field of urban water space landscape design is still in its infancy, and the interpretation of its concept is also different. The ecological design of the landscape reflects a new dream of human beings, a new aesthetics and value: the true cooperative and fraternal relationship between man and nature. At present, the ecological design of urban water space landscape has not put forward a more accurate concept, clear principles and standards, and a complete and systematic theoretical basis, which requires further research, discussion, and continuous practice by this generation of designers to improve it. To this end, this paper proposed a research method on the integration of water environment governance in urban and rural spaces with natural ecological landscape design. This paper mainly talked about the status quo of water environment and its network sensor algorithm research and analyzed its coverage area one by one. Then, the water quality extraction is introduced in detail. And finally, the data analysis of the Beijing river waters, the analyzer rainfall, water quality, and so on are carried out in the experimental part. It could be seen from the experimental results that there were currently 22 reclaimed water plants in six urban areas of Beijing, with a daily water treatment capacity of 4.08 million cubic meters and a sewage treatment rate of 98%. As of 2016, 440 million cubic meters of reclaimed water has been reused. With the commissioning of the new reclaimed water system, the proportion of reclaimed water in the river and lake environment will continue to increase.
Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer
Authors: Wang Jingmiao , Zhang Haizhong , Yin Xiaoyan , Bian Yanrui
Published: September 2022

Background. Cisplatin resistance is observed in patients with laryngeal cancer. The present study was designed to explore the efficacy of oxaliplatin on laryngeal cancer and elucidate the underlying mechanisms. Methods. Cell viability was determined by using MTT assays. Cell apoptosis was determined by using annexin V and propidium iodide (PI) staining. Flow cytometry and immunofluorescence were applied to determine the levels of calreticulin (CALR) and DiD (1,1-dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine). Flow cytometry was applied to analyze the levels of CD83, CD86, IFN-γ-producing CD8+ T cells, and CD4+CD25+FoxP3+ Tregs. The levels of adenosine triphosphate (ATP) were determined by using a chemiluminescent ATP kit and cytokines were determined by using specific enzyme-linked immunosorbent assays (ELISAs). The levels of HMGB1 were determined by using Western blot and ELISA, respectively. The xenograft animal model was constructed to evaluate the antitumor effects of oxaliplatin. Results. Oxaliplatin inhibited cell growth, promoted cell apoptosis, and induced the levels of CALR, ATP, and high mobility group box protein 1 (HMGB1) in Hep-2 cells. Oxaliplatin-treated Hep-2 cells increased the intensity of DiD and the levels of CD83 and CD86 in dendritic cells (DCs), as well as induced the supernatant IL-6 and TNF-α. Oxaliplatin-treated primary laryngeal cancer cell-pulsed DCs increased the IFN-γ-producing CD8+ T cells and suppressed CD4+CD25+FoxP3+ Tregs. In vivo data showed that oxaliplatin suppressed tumor growth and increased the populations of CD86+CD80+ and CD8+CD45+ cells in the tumor tissues. Conclusion. Treatment with oxaliplatin inhibited laryngeal cancer cells by inducing immunogenic cell death.
Therapeutic Effect of Curcumol on Chronic Atrophic Gastritis (CAG) and Gastric Cancer Is Achieved by Downregulating SDF-1α/CXCR4/VEGF Expression
Authors: Ma Xuehui , Zhang Zhengbo , Qin Xiayu , Kong Lingjing , Zhu Wen , Xu Lingzhi , Zhou Xin
Published: September 2022

CAG is an essential procession of the transformation from gastritis into gastric cancer. A series of timely moves of diagnosis, treatment, and monitoring towards CAG to anticipate the potential population at risk of gastric cancer is an effective means to prevent gastric cancer occurrence. The main active monomer in Fuzheng Huowei Decoction is Curcumol, which is an indispensable ingredient in the treatment to CAG and gastric cancer. In this study, the CAG model, in vitro cultured gastric cancer cells, and participating nude mice were treated with Curcumol, and alterations in SDF-1α/CXCR4/VEGF expression were estimated using the assays of immunohistochemistry and Western blot. MTT, flow cytometry, transwell, HE staining, and tumor volume determination were applied for the verification of the regulatory effects of Curcumol on CAG and gastric cancer cells. The results showed that the expressions of VEGF, SDF-1α, CXCR4, and CD34 decreased in our CAG model with Curcumol treatment. Curcumol is in procession of an inhibitory effect toward the activity, migration, and invasion of gastric cancer cells, and it would also result in gastric cancer cells’ apoptosis. We subsequently added SDF-1α overexpressing lentivirus to the Curcumol-treated group and found that the expressions of SDF-1α, CXCR4, and VEGF protein increased, and the inhibitory effect of Curcumol on gastric cancer cells was withdrawn. Our nude mouse experiment showed that Curcumol + SDF-1α group ended up with the largest tumor volume, while Fuzheng Huowei + NC group was with the smallest tumor volume. In conclusion, Curcumol is able to effectively protect the gastric tissue and suppress gastric cancer cells’ viability. Curcumol functions as a therapeutic factor in chronic atrophic gastritis and gastric cancer by downregulating SDF-1α/CXCR4/VEGF expression.
[Retracted] Progress and Emotion Expression of Campus Cultural Innovation Goods Based on Data Mining under Culture Shock Environment
Authors: Zhang Liyuan
Published: September 2022

With the rapid progress of economy and globalization, the characteristic culture of universities is inevitably eroded by the strong mainstream culture from outside. The best way to maintain culture is to turn it into goods. Productization can make culture vivid and vivid and circulate in use. Universities are the carrier of education and the progress of excellent culture. Campus culture refers to all tangible and intangible objective existence created artificially and formed for a long time in a specific school environment, which can deeply reflect the characteristics of the school. This article proposes an optimized design scheme for the progress and emotional expression research of campus cultural innovative goods in universities. It constructs the graphics recognition of campus cultural goods through the data mining system and then focuses on the problem of graphics recognition based on DL (deep learning). An enhanced DL model and arithmetic are proposed to enhance the accuracy and efficiency of graphics recognition. Finally, the simulation test and analysis are carried out. The simulation results show that the proposed arithmetic has certain accuracy, which is 10.23% higher than the traditional DL arithmetic. Campus culture can also play a powerful role in emotional edification and personality shaping for teachers, students, and campus workers. Excellent cultural and innovative goods can inherit the history, culture, and spirit of universities and can directly reflect the cultural heritage, characteristics, and graphics of universities. In addition to promoting the precipitation and inheritance of the long history and culture of universities and the spirit of the times, they can also strengthen the cultural exchange and spiritual communication among college level, alumni, teachers, and students, and people from all walks of life in universities, so as to actively promote the cultural and spiritual construction of universities.
Extracellular Vesicles in Trypanosoma cruzi Infection: Immunomodulatory Effects and Future Perspectives as Potential Control Tools against Chagas Disease
Authors: Cortes-Serra Nuria , Gualdron-Lopez Melisa , Pinazo Maria-Jesus , Torrecilhas Ana Claudia , Fernandez-Becerra Carmen
Published: September 2022

Chagas disease, caused by the protozoa parasite Trypanosoma cruzi, is a neglected tropical disease and a major public health problem affecting more than 6 million people worldwide. Many challenges remain in the quest to control Chagas disease: the diagnosis presents several limitations and the two available treatments cause several side effects, presenting limited efficacy during the chronic phase of the disease. In addition, there are no preventive vaccines or biomarkers of therapeutic response or disease outcome. Trypomastigote form and T. cruzi-infected cells release extracellular vesicles (EVs), which are involved in cell-to-cell communication and can modulate the host immune response. Importantly, EVs have been described as promising tools for the development of new therapeutic strategies, such as vaccines, and for the discovery of new biomarkers. Here, we review and discuss the role of EVs secreted during T. cruzi infection and their immunomodulatory properties. Finally, we briefly describe their potential for biomarker discovery and future perspectives as vaccine development tools for Chagas Disease.
Effect of Coronavirus Disease (COVID‐19) on Human Semen: No Evidence of Coronavirus in Semen of Patients
Authors: Vahidi Serajoddin , Nabi Ali , Alipoor Hojat , Karami Hormoz , Rahavian Amirhossein , Ayatollahi Alireza , Marvast Laleh Dehghan , Abouei Saeid
Published: September 2022

Background. In December 2019, a severe acute respiratory syndrome (SARS‐COV‐2) was found in China. The coronavirus can impact different organs, as shown by the virus having been detected in urine, blood, oropharyngeal, and feces. This study was done to assess the impact of COVID‐19 on semen analysis and to evaluate the existence of the virus in the semen of infected men. Methodology. Forty fertile men with COVID‐19 were confirmed by an oropharyngeal sample. The men were divided into two groups. The semen of twenty men in the acute stage of COVID‐19 and twenty men in the clinical recovery stage was analyzed, and the parameters of semen were compared between two groups. In addition, a PCR test of patients’ semen was done. Result. The analysis showed that all patients’ semen specimens tested negative. Semen analysis revealed no significant difference in sperm count, concentration, or motility, and the sperm of both groups was found to be normal. However, viability and morphology parameters were significantly lower in men with the acute disease. Conclusion. Coronavirus (COVID‐19) was not secreted in the semen of infected men but had a negative effect on the morphology and viability of the sperm of men in the acute stage.
Power Tracking and Energy Balancing of Energy Storage Systems under Unreliable Communication Network
Authors: Cai He , He Yaqi , Gao Huanli
Published: September 2022

In this paper, we consider the distributed power tracking and energy balancing problem of a general energy storage system subject to unreliable switching communication network. In order to deal with the uncertainty of the network topology, a distributed observer-based approach has been proposed. First, an adaptive distributed observer is employed to recover the reference power for the entire energy storage system for each energy storage unit. Second, based on the estimated reference power, a certainty equivalent control law is synthesized to simultaneously achieve power tracking and energy balancing. Numerical simulations are provided to validate the proposed control approach.
HLA‐DRA Gene Polymorphisms Are Associated with Graves’ Disease as an Autoimmune Thyroid Disease
Authors: Du Peng , Zhu Jiaoli , Yao Qiuming , Cai Tiantian , Xu Jianbin , Fang Yudie , Wu Yuqing , Zhang Wen , Zhang Jin-an
Published: September 2022

Background. Autoimmune thyroid disease (AITD), one of the most prevalent organ‐specific autoimmune diseases, mainly includes Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). This study was aimed at researching the association between AITD and single nucleotide polymorphisms (SNPs) of the HLA‐DRA gene. Methods. Using Hi‐SNP high‐throughput sequencing technology, we detected the distribution of three SNPs (rs3177928, rs7197, and rs3129878) of HLA‐DRA genotypes in 1033 AITD patients (634 GD and 399 HT ones) and 791 healthy volunteers in Chinese Han Population. Chi‐square test, multivariate logistic regression, and haplotype analysis were performed by SPSS and Haploview software to analyze the relationship between HLA‐DRA gene polymorphisms and AITD. Results. The results show that allele frequency and genotype distribution of rs3177928 and rs7197 were correlated with AITD and GD compared with the healthy control group, but not with HT. Rs3177928 and rs7197 were correlated with AITD and HT in the allele model, dominant model, and overdominant model before and after gender and age adjustment, but not with HT. In addition, we found that two loci (rs3177928 and rs7197) constituted a linkage disequilibrium (LD) region, and haplotype AA was associated with AITD and GD. However, we found no association between rs3129878 and AITD. Conclusion. Our study is the first to find that rs3177928 and rs7197 of HLA‐DRA are significantly correlated with AITD and GD in the Chinese Han population. This will help further reveal the pathogenesis of AITD and provide new candidate genes for the prediction or treatment of AITD.
Construction of Mathematical Model of Logistics Delivering Based on Intelligent Mobilization
Authors: Liang Haijun , Guo Jing
Published: September 2022

Distribution process is the core of logistics enterprise system. Efficient distribution process is the key for enterprises to improve logistics service level, gain competitive advantage, and win customers. In order to reduce the design error of the system and ensure the effective operation of the system, this paper establishes the system model and analyzes the properties of the model through the evaluation and analysis of various resources of the distribution system. The future development of intelligent logistics system is not only the key for logistics enterprises to win competition, but also a new measure to promote China’s economic development. This is not only a typical NP hard problem, but also a major challenge for the rational and scientific development of the intelligent logistics industry. Based on the previous theoretical research results, this paper intends to explore the intelligent logistics distribution route selection scheme by using mathematical modeling methods such as particle swarm optimization, so as to provide new technologies and methods for logistics distribution operation and management decision-making. The results show that compared with the traditional genetic algorithm, the accuracy of the improved particle swarm optimization algorithm is improved by 10.36%. This method effectively improves the operation cycle and link efficiency and achieves the purpose of optimization. The improved particle swarm optimization algorithm proposed in this paper is not only more suitable and effective for enterprise decision makers to deal with subjective judgments in an imprecise environment. It is also based on the evaluation sequence of each indicator of the alternative obtained from the evaluation and the comprehensive evaluation of all indicators. By weighting and considering the results, the best solution for enterprise logistics and distribution is selected.
Nanocurcumin Improves Lipid Status, Oxidative Stress, and Function of the Liver in Aluminium Phosphide‐Induced Toxicity: Cellular and Molecular Mechanisms
Authors: Salimi Ali , Kheiripour Nejat , Fathi Jouzdani Ali , Ghasemi Hassan , Soleimani Asl Sara , Ghafouri-Khosrowshahi Abolfazl , Ranjbar Akram
Published: September 2022

Background. The present study aimed to evaluate the effect of nanocurcumin and curcumin on liver transaminases, lipid profile, oxidant and antioxidant system, and pathophysiological changes in aluminium phosphide (ALP) induced hepatoxicity. Material and Methods. In this experimental study, thirty‐six male Wistar rats were randomly divided into six groups curcumin (Cur), nanocurcumin (Nanocur), ALP, ALP+Cur, and ALP+Nanocur. All treatments were performed by oral gavage for seven days. After treatment, animals were sacrificed, and liver and blood samples were taken. Serum levels of aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (AP), total bilirubin, cholesterol, triglyceride, high‐density lipoprotein (HDL), low‐density lipoprotein (LDL), and very‐low‐density lipoprotein (VLDL) were measured by photometric methods. Total antioxidant capacity (TAC) and malondialdehyde (MDA) as parameters of oxidative stress and mRNA expression of the nonenzyme protein including Sirtuin 1 (STR1), Forkhead box protein O1 (FOXO1) and protein O3 (FOXO3), catalase (CAT), and glutathione peroxidase (GPX) as the enzyme protein in homogenized tissues have been investigated. A histologist analyzed liver tissue sections after staining with hematoxylin‐eosin. Results. In the aluminium phosphide group, there was a significant increase in MDA, ALT, AST, and AP and total bilirubin, cholesterol, triglyceride, LDL, and VLDL; AST, ALT, total bilirubin, LDL, VLDL, cholesterol, and MDA were significantly decreased; and HDL and TAC were significantly increased compared to ALP (P < 0.05). In the ALP+Nanocur group, ALT, AST, ALP, total bilirubin, cholesterol, LDL, VLDL, triglyceride, and MDA were significantly decreased and HDL and TAC were increased significantly (P < 0.05). The effect of nanocurcumin on controlling serum levels of LDL, VLDL, triglyceride, and MDA in ALP‐poisoned rats was significantly more than curcumin (P < 0.05). The ALP group had significant changes in genes SIRT1, FOXO1a, FOXO3a, CAT, and GPX compared to healthy controls (P < 0.05). Nanocurcumin mice expressed more SIRT1, FOXO1a, CAT, and GPX genes than controls, and curcumin‐treated mice expressed more SIRT1 and FOXO1a genes (P < 0.05). Histopathological findings also indicated a more significant protective effect of nanocurcumin relative to curcumin against ALP‐induced hepatotoxicity. Conclusion. Nanocurcumin significantly protects the liver against aluminum phosphide toxicity. It is suggested that nanocurcumin‐based drugs be developed to reduce the toxic effects of ALP in poisoned patients.
Regulatory Role of B Cells and Its Subsets in Hepatitis E Virus Infection
Authors: Sharma Meenal , Tripathy Anuradha S.
Published: September 2022

Antibodies as well as memory B cells are the potential correlates of a protective immune response against hepatitis E virus (HEV) infection. Literature on the role of B regulatory cells (Bregs) in acute viral infections is limited. We have evaluated the role of IL‐10 expressing Bregs in HEV infection. A total of 108 acute hepatitis E patients, 55 hepatitis E recovered individuals and 128 HEV naïve healthy controls were enrolled. The percentages of peripheral CD19+, immature CD19+CD24hiCD38hi, mature CD19+CD24intCD38int and memory CD19+CD24hiCD38- B cells were analyzed by flowcytometry. Intracellular cytokine staining for IL‐10 and TGF‐β, HEV‐rORF2p specific T cell response (IFN‐γ expression) pre/post IL‐10/IL‐10R blocking and CD19+IL‐10+ B cells‐depletion based assays were carried out to assess the functionality of Bregs. The percentage of HEV‐rORF2p specific immature B cell phenotype was significantly higher in acute hepatitis E patients compared to hepatitis E recovered individuals and controls. Significantly higher IL‐10 expression on B and HEV‐rORF2p stimulated immature B cells of acute hepatitis E patients compared to controls indicated that Bregs are functional and HEV‐rORF2p specific. Enhanced IFN‐γ expression on CD8+ T cells upon IL‐10/IL‐10R blocking and also post CD19+IL‐10+ B cells depletion suggested that CD3+CD8+IFN‐γ+ T cells corroborate the regulatory potential of Bregs via IL‐10 dependent mechanism. We have identified HEV specific functional, immature CD19+CD24hiCD38hi B cells having IL‐10 mediated regulatory activities and a potential to modulate IFN‐γ mediated T cell response in Hepatitis E. The prognostic/pathogenic role of Bregs in recovery from severe hepatitis E needs evaluation.
Investigation of Applying Machine Learning and Hyperparameter Tuned Deep Learning Approaches for Arrhythmia Detection in ECG Images
Authors: Shanmugavadivel Kogilavani , Sathishkumar V. E. , Kumar M. Sandeep , Maheshwari V. , Prabhu J. , Allayear Shaikh Muhammad
Published: September 2022

The level of patient’s illness is determined by diagnosing the problem through different methods like physically examining patients, lab test data, and history of patient and by experience. To treat the patient, proper diagnosis is very much important. Arrhythmias are irregular variations in normal heart rhythm, and detecting them manually takes a long time and relies on clinical skill. Currently machine learning and deep learning models are used to automate the diagnosis by capturing unseen patterns from datasets. This research work concentrates on data expansion using augmentation technique which increases the dataset size by generating different images. The proposed system develops a medical diagnosis system which can be used to classify arrhythmia into different categories. Initially, machine learning techniques like Support Vector Machine (SVM), Naïve Bayes (NB), and Logistic Regression (LR) are used for diagnosis. In general deep learning models are used to extract high level features and to provide improved performance over machine learning algorithms. In order to achieve this, the proposed system utilizes a deep learning algorithm known as Convolutional Neural Network-baseline model for arrhythmia detection. The proposed system also adopts a novel hyperparameter tuned CNN model to acquire optimal combination of parameters that minimizes loss function and produces better result. The result shows that the hyper-tuned model outperforms other machine learning models and CNN baseline model for accurate classification of normal and other five different arrhythmia types.
Evaluation of the Modern Value of Imperial Examination Culture in the Context of Cultural Confidence Based on Deep Learning Models
Authors: Zhao Xiu
Published: September 2022

National prosperity, national spiritual independence, and cultural security greatly impact the cultural self-confidence. It is also a new era issue that must be answered in building a socialist cultural power. In this paper, we scientifically define the core concepts of the imperial examination system, imperial examination culture, and imperial examination cultural heritage. We systematically discuss the connotation and extension of the imperial examination cultural heritage, analyze the positive energy and negative effects of the imperial examination culture, propose the construction of a new type of examination culture system, and innovate the content and form of the examination. We conduct an in-depth study on the modern value of the imperial examination culture in the context of cultural confidence and then construct appropriate evaluation indicators to use the deep learning model to evaluate the value. The technical principle and performance optimization method of DBN neural network are introduced and then the modern value evaluation system of imperial examination culture is constructed. The parameters of the DBN model are optimally selected through experiments. After the optimal model is trained, the sample data are input to obtain the results. The results are compared with the evaluation results of human experts to judge the performance of the model. In many cases, the results are very close to, or the same as the expert results, in some of the experiments, the accuracy of our model varied from the expert results by a small margin of up to 0.4%.
The Tumor Microenvironment of Clear-Cell Ovarian Cancer
Authors: Devlin Michael-John , Miller Rowan , Laforets Florian , Kotantaki Panoraia , Garsed Dale W. , Kristeleit Rebecca , Bowtell David D. , McDermott Jacqueline , Maniati Eleni , Balkwill Frances R.
Published: September 2022

Some patients with advanced clear-cell ovarian cancer (CCOC) respond to immunotherapy; however, little is known about the tumor microenvironment (TME) of this relatively rare disease. Here, we describe a comprehensive quantitative and topographical analysis of biopsies from 45 patients, 9 with Federation Internationale des Gynaecologistes et Obstetristes (FIGO) stage I/II (early CCOC) and 36 with FIGO stage III/IV (advanced CCOC). We investigated 14 immune cell phenotype markers, PD-1 and ligands, and collagen structure and texture. We interrogated a microarray data set from a second cohort of 29 patients and compared the TMEs of ARID1A-wildtype (ARID1Awt) versus ARID1A-mutant (ARID1Amut) disease. We found significant variations in immune cell frequency and phenotype, checkpoint expression, and collagen matrix between the malignant cell area (MCA), leading edge (LE), and stroma. The MCA had the largest population of CD138+ plasma cells, the LE had more CD20+ B cells and T cells, whereas the stroma had more mast cells and αSMA+ fibroblasts. PD-L2 was expressed predominantly on malignant cells and was the dominant PD-1 ligand. Compared with early CCOC, advanced-stage disease had significantly more fibroblasts and a more complex collagen matrix, with microarray analysis indicating “TGFβ remodeling of the extracellular matrix” as the most significantly enriched pathway. Data showed significant differences in immune cell populations, collagen matrix, and cytokine expression between ARID1Awt and ARID1Amut CCOC, which may reflect different paths of tumorigenesis and the relationship to endometriosis. Increased infiltration of CD8+ T cells within the MCA and CD4+ T cells at the LE and stroma significantly associated with decreased overall survival.
Cerebellar Cortex Stimulation for Acquired Dystonia: A Case Report and Review of Its Role in Modern Surgical Practice
Authors: Stroud Anna , Tisch Stephen , Jonker Benjamin P.
Published: September 2022

Background: Cerebral palsy (CP) is a common cause of acquired dystonia, which can lead to significant interference with quality of life and societal participation. In the last two decades, the surgical treatment of dystonia has primarily focused on deep brain stimulation targeting the basal ganglia and thalamic circuits. However, stimulation of the basal ganglia has generally been less effective in acquired combined forms of dystonia, including dystonic CP. These limitations, along with growing evidence for the role of the cerebellum in the pathophysiology of dystonia, have led to renewed interest in the cerebellum as a target for therapeutic stimulation in dystonia. Nevertheless, there are very few contemporary studies demonstrating its use. We present the case of a patient with generalized dystonia due to dyskinetic CP who was successfully treated with stimulation of the cerebellar cortex in the modern era. We also review the evidence underpinning targeting of the cerebellum in surgical therapy for dystonia and examine the latest reports of this approach in the surgical literature. Summary: The patient derived significant improvement in the control of her dystonic symptoms, with a reduction in her BFMDRS score from 83 to 25. No complications were observed during more than 3 years of postoperative follow-up. Since the turn of the 21st century, there have been only 7 reports of cerebellar stimulation for dystonia, recruiting a total of 18 patients. These studies have exclusively targeted deep brain structures, making the present report of cortical cerebellar stimulation particularly unique. Key Messages: In the 21st century, cerebellar stimulation has predominantly been a second-line treatment for dystonia, after the failure of DBS targeting more mainstream loci within the thalamus and globus pallidus. However, there is increasing recognition of the role of the cerebellum in movement disorders, with multiple convergent lines of evidence supporting its involvement in dystonia pathophysiology. The cerebellum is worthy of greater consideration as a target for neurostimulation in dystonia, particularly in cases of acquired etiology.
The Growth Factors: Potential Biomarkers and Therapeutic Targets in Kidney Diseases
Authors: Tang Wei , Zhang Yufeng , Cui Sijia , Yi Fan
Published: September 2022

Background: Kidney diseases are a prevalent health problem worldwide. Although substantial progress has been made in understanding the pathophysiology of kidney disease, currently there is no satisfactory clinical treatment available to prevent or treat kidney disease. Therefore, strategies to establish early diagnosis, identify the key molecules, and develop novel therapeutic interventions to slow the progression of kidney diseases and reduce their complications are encouraged. Summary: The growth factors play a crucial role in the development of kidney diseases. The altered levels of growth factors are usually detected in circulation and urine in the disease course. A growing body of studies has suggested that growth factors, receptors, and related regulators are promising biomarkers for the diagnosis and/or prognosis and potential therapeutic targets for the treatment of kidney diseases. In this review, we summarize recent advances in the potential applications of growth factors for diagnostic biomarkers and therapeutic targets in kidney diseases and highlight their performances in clinical trials. Key Messages: Most diagnostic and therapeutic strategies targeting growth factors are still far from clinical implementation. The better understanding of growth factor-regulated pathophysiology and the progress of new intervention approaches are expected to facilitate the clinical translation of growth factor-based diagnosis and therapy of kidney diseases.
Care4AD: A Technology-Driven Platform for Care Coordination and Management: Acceptability Study in Dementia
Authors: Mishra Ram Kinker , Park Catherine , Momin Anmol Salim , Rafaei Nesreen El , Kunik Mark , York Michele K. , Najafi Bijan
Published: September 2022

Introduction: The technology-driven solution can reduce the caregiving burden; however, the needs of dementia caregiving are unique, and attitudes towards adopting technology from the perspectives of all the stakeholders involved in dementia caregiving are unclear. This study aims to assess the acceptability and feasibility of a technology-driven platform to facilitate care coordination platform, Care4AD, from the end-user perspective. Methods: Care4AD includes three components: (1) Care4AD app: the app is used by caregivers to coordinate care, monitor physical activity, and schedule reminders; (2) Care4AD tablet: a smart tablet is used by the care recipient to display scheduled reminders; and (3) Care4AD tags: a series of wireless sensor tags attached to various objects of daily care to facilitate monitoring instrumental activities of daily living (IADL) and adherence to scheduled tasks. Stakeholders in caregiving, including 11 experts in dementia care (age: 53.3 ± 8, 73% female), 10 individuals with dementia (IWD) (age: 76.1 ± 7.3, 50% female), and 14 caregivers (age: 66.9 ± 10.6, 75% female) were interviewed to determine perceived ease of use, attitude towards use, and perceived usefulness, based on the technology acceptance model (TAM) questionnaire. Additionally, we assessed technology anxiety and concerns with data sharing by caregivers and IWD. The interviews were conducted through videoconferencing or in-person meetings. The interview was composed of open-ended questions, a demonstration of the proposed Care4AD platform, and a survey based on TAM. Results: Compared to the neutral response, stakeholders showed significantly higher acceptance (70–100% satisfied to highly satisfied, p < 0.05) for all components of the TAM. Among IWD, age (r = −0.68, p = 0.03) and for caregivers the perceived ease of use (r = 0.73, p < 0.01) were significant predictors of attitude towards using the technology. Interestingly, neither concerns about data sharing nor educational level were limiting factors in the acceptability of the system in our sample. Conclusion: Overall, the results support a high perception of usefulness, ease of use, and attitude towards using Care4AD. The key barriers to adopting such technology are the age of IWD and the caregiver’s perception of ease of use. Future studies are warranted to explore the effectiveness of such a platform to reduce caregiver stress and improve the quality of life and independence of IWD.
Endoscopic Management of a Chronic Gastrocutaneous Fistula after Bariatric Revisional Surgery Using a Novel Cardiac Septal Occluder
Authors: Kumaira Fonseca Mariana , Coelho Nelson Heitor Vieira , Manica João Luiz Langer , Ramblo Rafael Ramos , Spier Ingrid Elisa , Seabra Artur Pacheco
Published: September 2022

Introduction: Endoscopic techniques are now considered the first-line approach for the management of bariatric surgery-related fistulas. The off-label use of cardiac septal defect occluders (CSDO) is an emerging technique that has demonstrated favorable outcomes for the closure of extravascular defects, including gastrointestinal (GI) disruptions. Previous case reports have reported similar results with the CSDO Amplatzer™ for the management of GI disruptions following bariatric surgery. However, the use of similar alternative devices for this purpose has not yet been described. Case Presentation: This case report presents the first reported use of the Occlutech® CSDO for the treatment of a chronic gastrocutaneous fistula after bariatric revisional surgery. Despite apparent initial success – no extravasation of contrast material through the device in the contrast study after the CSDO placement – fistula closure failed due to partial dislodgement of the device. The placement of a second device between the discs of the former one ultimately sealed the fistulous orifice. Discussion: In chronic GI fistulas, the mature tract is often not liable to the application of standard endoscopic methods, leading to failed closure attempts. A new application of Occlutech® CSDO can obviate the clinical burden of a high-risk laparotomy in these cases. Appropriate endoscopic equipment as well as the involvement of a multidisciplinary team are prime conditions to ensure successful patient outcomes.
Language in Healthy Ageing: A Comparison across Language Domains
Authors: Biran Michal , Gvion Aviah , Shmuely-Samuel Shira
Published: September 2022

Introduction: Life expectancy has been increasing in recent decades. Therefore, it is important to understand the functional changes during healthy ageing. Most research has mainly focused on one linguistic domain at a time. The current study aimed at investigating whether changes in language performance in healthy ageing occur in some language domains more than in others. Methods: Twenty-three older healthy Hebrew-speaking adults, exhibiting no cognitive decline, were examined on tasks aimed at testing their performance in different language and cognitive domains: lexical retrieval, complementation information, syntax, theory of mind (a domain related to pragmatic aspects of language), and short-term memory. We compared their performance at both the group and the individual levels. Comparisons were made between the performance of the older adults and control data of young adults, and between the older adults’ performance in the different linguistic domains. In addition, correlations between the older adults’ phonological short-term memory abilities and their performance in various linguistic domains were examined. Results: A decline was found in several linguistic domains among the older adults, while in other domains no decline was found. However, no unequivocal decline in linguistic functioning was found due to relatively large variance in their performance. Conclusion: Not all linguistic domains are equally vulnerable in ageing, and not all older adults are equally affected. The research has both clinical and theoretical implications.
Criação do Museu do Instituto de Química da Universidade Federal do Rio Grande do Sul (UFRGS)
Authors: Cuty Jeniffer Alves , Schrekker Henri Stephan , Bertotto Márcia Regina
Published: September 2022

Este artigo aborda a criação do Museu do Instituto de Química (MIQ) da Universidade Federal do Rio Grande do Sul (UFRGS), propondo a problematização acerca dos desafios institucionais e do âmbito coletivo e social de implementação de um museu. Apresentamos a trajetória de trabalho multidisciplinar nesta constituição, que envolve docentes, técnicos e discentes dos cursos de Química, Museologia, Arquivologia, História e Publicidade e Propaganda. Contamos com a adesão de profissionais do patrimônio, parceria que está se constituindo nas ações firmadas por sua direção. Debatemos a eficácia da Rede de Museus e Acervos da UFRGS no apoio técnico e político, bem como propomos uma reflexão sobre o papel do MIQ em tempos de pandemia e crise multidimensional no Brasil. Operamos com conceitos centrais da Museologia, da Gestão de Museus, da teoria simmeliana relativa à crise e ao conflito em grupos sociais, da noção de “necropolítica” e de aproximações às noções de ethos do campo de conhecimento em questão e de suas linguagens específicas.
Reavaliando o acervo de artes aplicadas no MAC USP: a experiência do grupo de pesquisa “Narrativas da Arte do século XX” com a mostra “Projetos para um Cotidiano Moderno no Brasil, 1920-1960”
Authors: Magalhães Ana Gonçalves , Freitas Patrícia Martins Santos , Rocco Renata Dias Ferraretto Moura , Brognara Gustavo , Ronqui Andrea Augusto , Silva Mariana Leão , Vallego Rachel , Murari Victor Tuon , Faria Breno Marques Ribeiro de , Caffé Juliana , Salles Milena de
Published: September 2022

Este artigo tem por objetivo apresentar os processos de pesquisa, curadoria e reflexões do grupo “Narrativas da Arte do século XX” – coordenado pela Profa. Dra. Ana Gonçalves Magalhães – que geraram a mostra Projetos para um cotidiano moderno no Brasil, 1920-1960, ocorrida no Museu de Arte Contemporânea da Universidade de São Paulo (MAC-USP) entre agosto de 2021 e julho de 2022. O artigo se divide em duas partes: na primeira, todas as etapas do processo são descritas, desde o nascimento da ideia da exposição até seu desenvolvimento e quais agentes e em quais momentos foram envolvidos – pesquisadores internos e externos ao MAC-USP, outras áreas do museu, instituições e colecionadores particulares; na segunda parte são apresentados os textos que foram desenvolvidos para a exposição, ou seja, fruto da investigação apresentada na primeira parte.
Coleções biológicas da Universidade Federal de Rondônia: o Programa de Visitação na Socialização do Conhecimento Científico
Authors: Silveira Maria Aurea Pinheiro de Almeida , Messias Mariluce Rezende , Bígio Narcísio Costa , Silveira Antônio Laffayete Pires da , Andriolo Aline , Doria Carolina Rodrigues da Costa
Published: September 2022

A Universidade Federal de Rondônia é referência importante relacionada à biodiversidade da Amazônia em razão de suas coleções biológicas, que apresentam significativo acervo com foco na região sul-ocidental do bioma. Essas coleções têm papel relevante para que a Universidade se destaque no tripé ensino, pesquisa e extensão. Este artigo tem como objetivos descrever e refletir sobre o processo de implantação e implementação do Programa de Visitação às Coleções Biológicas da Unir, frente aos desafios de socialização do conhecimento da biodiversidade do estado de Rondônia; e relacionar as possiblidades e desafios para intensificação das atividades de extensão. As coleções e suas ações na área de extensão foram estabelecidas na última década por meio do programa de visitas orientadas por roteiro interpretativo à coleção didática e acervos, e se intensificaram nos últimos anos (mil alunos/ visitantes/ano). Os desafios para os próximos períodos envolvem produção de materiais didáticos mais interativos (físicos e virtuais), elaboração e promoção de atividades virtuais e curso de formação de monitores. Os resultados demonstram a importância de a ciência ir além dos muros da academia, e que ela deve ser transferida para toda a sociedade com metodologias apropriadas, em espaços formais e não formais de educação. Conclui-se que o conhecimento da biodiversidade local é favorecido com a presença de coleções biológicas e museus que podem ser acessadas pela população.
The Rudgea hostmanniana complex (Rubiaceae) in the Guiana Shield region
Authors: LACHENAUD OLIVIER , BRUNIERA CARLA P. , ZAPPI DANIELA C.
Published: September 2022

A taxonomic revision of the Rudgea hostmanniana complex in the Guiana shield region is presented. This group includes species with sheathing stipules bearing dorsal appendages, thick glabrous leaves lacking domatia, inflorescences thyrsoid to glomerulate but not regularly dichotomous, a calyx tube absent or very short, a corolla tube 2–7(–13) mm long, and fruits usually brownish when immature and turning red or orange at maturity. Eight species are recognized, among which Rudgea billietiae, endemic to Kaw Mountain in French Guiana, is newly described and illustrated; it is assessed as Endangered according to IUCN criteria. The other species are R. bolivarensis, R. cornigera, R. coussareoides, R. hostmanniana, R. maypurensis, R. pungens, and R. tanaosepala. A key to the species is presented, as well as a table summarising their diagnostic characters.
Taxonomic revision of Eunotia luna var. aequalis f. paucistriata Frenguelli and E. luna var. aequalis f. major Frenguelli: analysis of type material, lectotypification and current taxonomic status
Authors: GUERRERO JOSÉ M. , VOUILLOUD AMELIA A.
Published: September 2022

The valve morphology of the infraspecific taxa of Eunotia luna reported by J. Frenguelli for the Esteros del Iberá (Argentina) (E. luna var. aequalis, E. luna var. aequalis f. paucistriata and E. luna var. aequalis f. major) were examined to elucidate their taxonomic position. As result of the study with light and electron microscopy, we concluded that E. luna var. aequalis sensu Frenguelli and E. luna var. aequalis f. paucistriata are in fact the extremes of the range of variation of the same taxon, here considered as synonym of Eunotia deficiens. E. luna var. aequalis f. major shows a distinct valve morphology and it is therefore raised to the status of species as Eunotia silviasalae nom. nov. Since Frenguelli did not designate holotypes for E. luna var. aequalis f. paucistriata and E. luna var. aequalis f. major, we designate herein the corresponding lectotypes.
Two new genera of Sclerodermini (Hymenoptera: Bethylidae; Epyrinae) with large, scolebythid-like prosternums
Authors: AZEVEDO C. O.
Published: September 2022

The following new taxa are proposed, described and illustrated: Megaprosternum gen. nov. including M. longiceps sp. nov. from Fiji and M. pentagonal sp. nov. from Australia; and Solepyris gen. nov. including S. unicus sp. nov. from Brazil and Ecuador. Keys are provided for the genera of Sclerodermini and for the species of Megaprosternum .
Exaerete salsai sp. n. (Hymenoptera: Apidae): a new orchid bee from eastern Brazil
Authors: NEMÉSIO ANDRÉ
Published: September 2022

Exaerete salsai sp. n. (Hymenoptera: Apidae: Apini: Euglossina) is described from southern Bahia and northeastern Minas Gerais, eastern Brazil. Its relationships with the other members of the genus are discussed, and it is considered to be a close ally of the Amazonian Exaerete trochanterica (Friese, 1900) and the Central American Exaerete kimseyae Oliveira, 2011.
A new Leptobrachium (Anura: Megophryidae) from the highlands of southeastern Laos
Authors: STUART BRYAN L. , PHIMMACHAK SOMPHOUTHONE , SEATEUN SENGVILAY , SIVONGXAY NIANE
Published: September 2022

Twelve species of Leptobrachium are known from Vietnam, Laos and Cambodia, most of which have been described toscience in the past twelve years. Our fieldwork on the Dakchung Plateau of southeastern Laos discovered a small Lepto-brachium with a yellow upper iris that resembled L. buchardi, a species known only by the female holotype from the Bo-laven Plateau of southern Laos. We use our new collections to expand the original description of L. buchardi, and describethe Dakchung species as new. Leptobrachium xanthops sp. nov. is distinguished from its congeners by having small bodysize (males with SVL 44.7, females with SVL 38.8–45.2), the upper one-half of iris and scleral arc pale yellow, a dark venter, and sexually active males without spines on the upper lip.
A new Physoschistura from a Salween affluent in western Yunnan (Teleostei: Nemacheilidae)
Authors: ENDRUWEIT MARCO
Published: September 2022

Physoschistura absumbra, new species, is described from the watershed of the Salween in Cangyuan County, Yunnan Province, China. The combination of a complete lateral line, a continuous lower jaw, and the absence of a suborbital lobe distinguishes it from all other congeners. Further diagnostic characters are a long (dorsally 23–25% SL, laterally 26–28% SL) and deep head (at nape 13–14% SL), a shallow body (15–17% SL), a deep caudal peduncle (13–14% SL), 9–12 rather regular flank bars, a complete basicaudal bar, and a maximum adult size of about 44 mm SL.