bluuebunny/crossref_metadata_2025 huggingface dataset:
Leveraging single-cell transcriptomic data to uncover immune suppressive cancer cell subsets in triple-negative canine breast cancers
Authors: Kim Myung-Chul , Borcherding Nicholas , Song Woo-Jin , Kolb Ryan , Zhang Weizhou
Published: September 2024

Introduction

Single-cell RNA sequencing (scRNA-seq) has become an essential tool for uncovering the complexities of various physiological and immunopathological conditions in veterinary medicine. However, there is currently limited information on immune-suppressive cancer subsets in canine breast cancers. In this study, we aimed to identify and characterize immune-suppressive subsets of triple-negative canine breast cancer (TNBC) by utilizing integrated scRNA-seq data from published datasets.

Methods

Published scRNA-seq datasets, including data from six groups of 30 dogs, were subjected to integrated bioinformatic analysis.

Results

Immune modulatory TNBC subsets were identified through functional enrichment analysis using immune-suppressive gene sets, including those associated with anti-inflammatory and M2-like macrophages. Key immune-suppressive signaling, such as viral infection, angiogenesis, and leukocyte chemotaxis, was found to play a role in enabling TNBC to evade immune surveillance. In addition, interactome analysis revealed significant interactions between distinct subsets of cancer cells and effector T cells, suggesting potential T-cell suppression.

Discussion

The present study demonstrates a versatile and scalable approach to integrating and analyzing scRNA-seq data, which successfully identified immune-modulatory subsets of canine TNBC. It also revealed potential mechanisms through which TNBC promotes immune evasion in dogs. These findings are crucial for advancing the understanding of the immune pathogenesis of canine TNBC and may aid in the development of new immune-based therapeutic strategies.
Computed tomography radiomics models of tumor differentiation in canine small intestinal tumors
Authors: Jeong Jeongyun , Choi Hyunji , Kim Minjoo , Kim Sung-Soo , Goh Jinhyong , Hwang Jeongyeon , Kim Jaehwan , Cho Hwan-Ho , Eom Kidong
Published: September 2024

Radiomics models have been widely exploited in oncology for the investigation of tumor classification, as well as for predicting tumor response to treatment and genomic sequence; however, their performance in veterinary gastrointestinal tumors remains unexplored. Here, we sought to investigate and compare the performance of radiomics models in various settings for differentiating among canine small intestinal adenocarcinoma, lymphoma, and spindle cell sarcoma. Forty-two small intestinal tumors were contoured using four different segmentation methods: pre- or post-contrast, each with or without the inclusion of intraluminal gas. The mesenteric lymph nodes of pre- and post-contrast images were also contoured. The bin settings included bin count and bin width of 16, 32, 64, 128, and 256. Multinomial logistic regression, random forest, and support vector machine models were used to construct radiomics models. Using features from both primary tumors and lymph nodes showed significantly better performance than modeling using only the radiomics features of primary tumors, which indicated that the inclusion of mesenteric lymph nodes aids model performance. The support vector machine model exhibited significantly superior performance compared with the multinomial logistic regression and random forest models. Combining radiologic findings with radiomics features improved performance compared to using only radiomics features, highlighting the importance of radiologic findings in model building. A support vector machine model consisting of radiologic findings, primary tumors, and lymph node radiomics features with bin count 16 in post-contrast images with the exclusion of intraluminal gas showed the best performance among the various models tested. In conclusion, this study suggests that mesenteric lymph node segmentation and radiological findings should be integrated to build a potent radiomics model capable of differentiating among small intestinal tumors.
Rapid Lactic Acid Content Detection in Secondary Fermentation of Maize Silage Using Colorimetric Sensor Array Combined with Hyperspectral Imaging
Authors: Xue Xiaoyu , Tian Haiqing , Zhao Kai , Yu Yang , Xiao Ziqing , Zhuo Chunxiang , Sun Jianying
Published: September 2024

Lactic acid content is a crucial indicator for evaluating maize silage quality, and its accurate detection is essential for ensuring product quality. In this study, a quantitative prediction model for the change of lactic acid content during the secondary fermentation of maize silage was constructed based on a colorimetric sensor array (CSA) combined with hyperspectral imaging. Volatile odor information from maize silage samples with different days of aerobic exposure was obtained using CSA and recorded by a hyperspectral imaging (HSI) system. Subsequently, the acquired spectral data were subjected to preprocessing through five distinct methods before being modeled using partial least squares regression (PLSR). The coronavirus herd immunity optimizer (CHIO) algorithm was introduced to screen three color-sensitive dyes that are more sensitive to changes in lactic acid content of maize silage. To minimize model redundancy, three algorithms, such as competitive adaptive reweighted sampling (CARS), were used to extract the characteristic wavelengths of the three dyes, and the combination of the characteristic wavelengths obtained by each algorithm was used as an input variable to build an analytical model for quantitative prediction of the lactic acid content by support vector regression (SVR). Moreover, two optimization algorithms, namely grid search (GS) and crested porcupine optimizer (CPO), were compared to determine their effectiveness in optimizing the parameters of the SVR model. The results showed that the prediction accuracy of the model can be significantly improved by choosing appropriate pretreatment methods for different color-sensitive dyes. The CARS-CPO-SVR model had better prediction, with a prediction set determination coefficient (RP2), root mean square error of prediction (RMSEP), and a ratio of performance to deviation (RPD) of 0.9617, 2.0057, and 5.1997, respectively. These comprehensive findings confirm the viability of integrating CSA with hyperspectral imaging to accurately quantify the lactic acid content in silage, providing a scientific and novel method for maize silage quality testing.
Real-Time ConvNext-Based U-Net with Feature Infusion for Egg Microcrack Detection
Authors: Shi Chenbo , Li Yuejia , Jiang Xin , Sun Wenxin , Zhu Changsheng , Mo Yuanzheng , Yan Shaojia , Zhang Chun
Published: September 2024

Real-time automatic detection of microcracks in eggs is crucial for ensuring egg quality and safety, yet rapid detection of micron-scale cracks remains challenging. This study introduces a real-time ConvNext-Based U-Net model with Feature Infusion (CBU-FI Net) for egg microcrack detection. Leveraging edge features and spatial continuity of cracks, we incorporate an edge feature infusion module in the encoder and design a multi-scale feature aggregation strategy in the decoder to enhance the extraction of both local details and global semantic information. By introducing large convolution kernels and depth-wise separable convolution from ConvNext, the model significantly reduces network parameters compared to the original U-Net. Additionally, a composite loss function is devised to address class imbalance issues. Experimental results on a dataset comprising over 3400 graded egg microcrack image patches demonstrate that CBU-FI Net achieves a reduction in parameters to one-third the amount in the original U-Net, with an inference speed of 21 ms per image (1 million pixels). The model achieves a Crack-IoU of 65.51% for microcracks smaller than 20 μm and a Crack-IoU and MIoU of 60.76% and 80.22%, respectively, for even smaller cracks (less than 5 μm), achieving high-precision, real-time detection of egg microcracks. Furthermore, on the publicly benchmarked CrackSeg9k dataset, CBU-FI Net achieves an inference speed of 4 ms for 400 × 400 resolution images, with an MIoU of 81.38%, proving the proposed method’s robustness and generalization capability across various cracks and complex backgrounds.
Assessing Economic Viability of Resilient Sheep Foraging Alternatives in Lowland Regions of Romania
Authors: Chetroiu Rodica , Rodino Steliana , Dragomir Vili , Ilie Diana Maria , Marin Ancuța
Published: September 2024

Sheep farming is an important branch of the animal husbandry sector in Europe. In 2023, Romania’s sheep and goat herds ranked third in the European Union, with more than 10 million heads, contributing substantially to the production of meat, traditional cheeses, and wool. However, in the current climate context, with extreme weather events and especially long periods of drought, providing optimal fodder ratio becomes a challenge for farmers. As animal nutritionists provide valid alternatives, consistent with the nutritional requirements of the species, the present work aims to present some economically efficient fodder alternatives to use for milk production in sheep farms of different sizes. The study brings added value to research in the field of using fodder alternatives in animal nutrition through the economic aspects researched because profitability is pursued in any activity, thus completing the technical arguments of previous studies in the literature in the field. Several economic indicators were calculated, such as the total value by categories of expenses, the value of production, the level of profit, and the rate of gross and net return. The analysis demonstrated that regardless of their size, the sheep farms located in lowland areas can reach positive values of profitability indicators when using alternative fodder in animal feeding.
Infection Process of Alfalfa Root Rot Caused by Fusarium acuminatum
Authors: Wang Le , Yang Jianfeng , Jia Ruifang , Chen Zhengqiang , Wang Na , Wu Jie , Chen Fangqi , Zhang Yuanyuan , Lin Kejian
Published: September 2024

Fusarium spp. can cause root rot in alfalfa, leading to the death of the whole plant, which seriously affects the yield and quality of alfalfa. This study used a Fusarium acuminatum strain labeled with green fluorescent protein (GFP) to observe the infection process of F. acuminatum on alfalfa by confocal fluorescence microscopy. The aim of this study was to reveal the infection mechanism of alfalfa Fusarium root rot at the cellular histological level. The results showed that conidia of F. acuminatum attached to the surface of the root and germinated at one day post-inoculation, the mycelium then entered the vascular bundle tissue of the alfalfa root at 5 days post-inoculation, reached the base of the plant stem at 14 days post-inoculation, and colonized the stem of the first and second compound leaf at 28 and 49 days post-inoculation, respectively. Moreover, the experiment, which sprayed a spore suspension, showed that the conidia of F. acuminatum could spread through the air to infect the pericarp and seed coat tissue of the pod. For the first time, we report the infection process of alfalfa Fusarium root rot caused by F. acuminatum and clarify that F. acuminatum can initially infect the root tissue of alfalfa, colonize the bottom stem of the plant through systematic infection, and eventually cause the plant to wilt and die. The results reveal the infection mechanism of F. acuminatum at the cell level via histology and provide theoretical support for the development of control strategies and key control technologies for alfalfa root rot.
A Comprehensive Benefit Evaluation of the Model of Salt-Tolerant Crops Irrigated by Mariculture Wastewater Based on a Field Plot Experiment
Authors: Chen Dan , Lu Huimin , Fu Ze , Luo Zhaohui , Duan Jiaming
Published: September 2024

The intensified development of aquaculture and excessive use of agricultural fertilizers pose a threat to natural resource availability and deteriorate the environment. Utilizing aquaculture wastewater from mariculture for agricultural irrigation can mitigate pollution and alleviate the pressure on natural resources. This study investigated the comprehensive benefits of using freshwater and mariculture wastewater for irrigation, employing two concentration levels of mariculture wastewater in a pot experiment with saline-tolerant rice. Furthermore, we quantitively assessed the integrated benefits for farmland by utilizing an ecosystem service function value assessment and emergy value theory. The results indicate a significant enhancement in the growth and yield of saline-tolerant rice when irrigated with mariculture wastewater. Specifically, the theoretical yield increased by 9.01% relative to freshwater irrigation. Irrigation using aquaculture wastewater significantly enhanced the nutrient concentrations in the soil, including soil organic carbon (SOC), avail-K (AK), Olsen-P (OP), and alkali-N (AN). Additionally, the uptake of these nutrients by salt-tolerant crops during their late reproductive stage effectively mitigated the rise in soil salinity induced by the wastewater irrigation practice. Under experimental conditions, wastewater irrigation conferred superior ecological benefits compared with freshwater irrigation. The comprehensive benefits of wastewater irrigation, valued at 104,439.10 RMB/hm2, exceeded those of freshwater irrigation by 188.8%. The utilization of mariculture wastewater effectively enhances the coastal environment, augments crop yields, and diminishes treatment costs. From the perspectives of revenue enhancement, environmental compatibility, and sustainability, the model of utilizing salt-tolerant crops irrigated with mariculture wastewater holds substantial promotional and practical significance.
Medium-Term Monitoring of Greenhouse Gases above Rice-Wheat Rotation System Based on Mid-Infrared Laser Heterodyne Radiometer
Authors: Xue Zhengyue , Li Jun , Shen Fengjiao , Zhang Sheng , Hu Xueyou , Tan Tu
Published: September 2024

The rice-wheat rotation system is a major agricultural practice in China as well as an important source of greenhouse gas (GHG) emissions. In this study, the developed mid-infrared laser heterodyne radiometer (MIR-LHR) was used for the remote sensing of atmospheric CH4 and N2O concentrations above the rice-wheat rotation system. From April 2019 to May 2022, the atmospheric column concentrations of CH4 and N2O above the rice-wheat rotation system were continuously observed in Hefei, China. The peak values of the N2O column concentration appeared 7~10 days after wheat seasonal fertilization, with additional peaks during the drainage period of rice cultivation. During the three-year rice-wheat crop rotation cycle, a consistent trend was observed in the CH4 column concentrations, which increased during the rice-growing season and subsequently decreased during the wheat-growing season. The data reveal different seasonal patterns and the impact of agricultural activities on their emissions. During the observation period, the fluctuations in the CH4 and N2O column concentrations associated with the rice-wheat rotation system were about 40 ppbv and 6 ppbv, respectively. The MIR-LHR developed for this study shows great potential for analyzing fluctuations in atmospheric column concentrations caused by GHG emissions in the rice-wheat rotation system.
Optimization of Clamping and Conveying Parameters for Spinach Orderly Harvesting with Low Damage by Simulation and Experiment
Authors: Wang Huankun , Qi Chong , Luo Qiaojun , Chen Minglin , Ma Yidong , Wang Xianlong
Published: September 2024

The leaves of spinach are delicate and easily injured during harvesting. To reduce the spinach damage rate and increase the conveyance success rate, an orderly harvester was designed and manufactured, and the key conveying parameters of the harvester were optimized by simulation and experiments. The compression damage stress of spinach was determined by compression tests. Then, a finite element simulation model for spinach clamping was established, and the influence of different clamping heights on the spinach deformation and equivalent stress were simulated and analyzed. Finally, response surface Box–Behnken experiments were conducted to optimize the combinations of the twisting angle, clamping distance, and height difference. The results of the compression tests showed that the compression damage stresses of spinach leaves, stems, and their connection points were 8.04 × 10−2 MPa, 7.85 × 10−2 MPa, and 11.63 × 10−2 MPa, respectively. The optimal clamping height of spinach for orderly conveyance was obtained to be 20 mm according to the finite element simulation. The response surface experimental results indicated that the significance order of factors affecting the extrusion force was the clamping distance, the height difference, and the twisting angle. The significance order of factors affecting the conveyance success rate was the clamping distance, the twisting angle, and the height difference. The optimal parameter combination was ae twisting angle of 60°, clamping distance of 24 mm, and a height difference of 20 cm. The experimental validation of the optimization results from the finite element simulation and response surface tests demonstrated that the extrusion force and conveyance success rate were 2.37 N and 94%, respectively, with a conveying damage rate of 3% for spinach, meeting the requirements for the low-damage and orderly harvesting of spinach.
Variation in Starch Properties in Ratoon Rice and Their Relationship with Rice Quality
Authors: Jiang Chenqian , Dai Shuaijun , Yang Dingguo , Huang Liying , Tian Xiaohai , Jin Zhaoqiang , Shi Shijie , Liu Ke , Zhang Yunbo
Published: September 2024

Ratoon rice is characterized by higher production combined with enhanced rice quality. However, the relationship between the differences in the structure and properties of the starch and variations in the ratoon season and rice quality is still unclear. In this study, we conducted a field experiment in 2023 to assess the relationship between rice quality differences and starch characteristics of four different hybrid rice varieties (Longjingyou1212: LJY1212; Taoyouxiangzhan: TYXZ; Liangyou6326: LY6326; Fengliangyouxiang1: FLYX1) in the ratoon season. In terms of appearance, the chalkiness degrees and chalky grain percentage of LJY1212 (0.6%, 2.3%) and TYXZ (1.2%, 6.0%) were 53.5% and 53.9% lower than those of LY6326 (2.4%, 10.0%) and FLYX1 (1.6%, 7.8%), indicating that the appearances of LJY1212 and TYXZ were better than those of LY6326 and FLYX1. The average amylose contents of LJY1212 (18.5%) and TYXZ (19.0%) were 10.8% higher than those of LY6326 (17.7%) and FLYX1 (15.7%), and there was no significant difference in protein content between the varieties in the ratoon season. Regarding the starch microstructure, the starch granules of LJY1212 and TYXZ in the ratoon season were arranged more closely. The average ratios of 1045/1022 cm−1 of LJY1212 (0.77) and TYXZ (0.76) were 7.0% higher than those of LY6326 (0.71) and FLYX1 (0.72), indicating that the internal orders of LJY1212 and TYXZ’s starch were better than those of LY6326 and FLYX1. The average relative crystallinities of LJY1212 (24.8%) and TYXZ (24.5%) were 5.2% lower than those of LY6326 (25.7%) and FLYX1 (26.6%). The relative crystallinity of starch decreased with the increase in amylose content, while the short-range order degree, gelatinization temperature, and gelatinization enthalpy increased with the increase in amylose content. Therefore, the LJY1212 and TYXZ varieties, with better rice qualities, are recommended for planting in southern China.
Development of Welfare Protocols at Slaughter in Farmed Fish
Authors: Mercogliano Raffaelina , Avolio Alessandro , Castiello Floriana , Ferrante Maria Carmela
Published: September 2024

The study investigated fish welfare at slaughter. Killing animals may induce suffering to the animals even under the best available technical conditions. Moreover, fish have different physiological characteristics and are slaughtered differently from terrestrial animals. The use of commercially available methods exposes farmed fish to pain and suffering during slaughter, which could lead to acute stress and post mortem changes in fish quality. The study aimed to discuss (i) the current knowledge and knowledge gaps on fish welfare related to stunning and killing methods; (ii) the variables that affect the post mortem changes in fish meat, and (iii) the indicators of welfare during slaughter. Application of welfare protocols at slaughter improves fish welfare. Specific protocols for fish are not provided in EC Regulation 1099/2009 on animal protection at killing. Detailed guidelines in the fish welfare assessment may allow the development of specific fish legislation. Developing humane technologies might have important effects on fish quality, consumer perception and aquaculture economics.
Longitudinal Study of Changes in Ammonia, Carbon Dioxide, Humidity and Temperature in Individually Ventilated Cages Housing Female and Male C57BL/6N Mice during Consecutive Cycles of Weekly and Bi-Weekly Cage Changes
Authors: Andersson Martina , Pernold Karin , Lilja Niklas , Frias-Beneyto Rafael , Ulfhake Brun
Published: September 2024

Housing conditions are essential for ensuring animal welfare and high-quality research outcomes. In this study, we continuously monitored air quality—specifically ammonia, carbon dioxide, relative humidity, and temperature—in Individually Ventilated Cages (IVCs) housing five female or male C57BL/6N mice. The cages were cleaned either weekly or bi-weekly, and the data were collected as the mice aged from 100 to 348 days. The survival rate remained above 96%, with body weight increasing by 35–52% during the study period. The ammonia levels rose throughout the cleaning cycle, but averaged below 25 ppm. However, in the older, heavier mice with bi-weekly cage cleaning, the ammonia levels reached between 25 and 75 ppm, particularly in the males. While circadian rhythms influenced the ammonia concentration only to a small extent, the carbon dioxide levels varied between 800 and 3000 ppm, increasing by 30–50% at night and by 1000 ppm with body weight. Humidity also correlated primarily with the circadian rhythms (10% higher at night) and, to a lesser extent, with body weight, reaching ≥70% in the middle-aged mice. The temperature variations remained minimal, within a 1 °C range. We conclude that air quality assessments in IVCs should be conducted during animals’ active periods, and both housing density and biomass must be considered to optimise welfare.
The Short-Term Impact of Educational Programs on Knowledge and Attitudes Regarding Antimicrobial Stewardship among Veterinary Students in Serbia
Authors: Kovačević Zorana , Čudina Nikola , Pećin Marko , Samardžija Marko , Pajić Marko , Pintarić Selma , Vlahek Ivan , Ružić Zoran , Vračar Vuk , Galić Ivan , Horvat Olga
Published: September 2024

Effective antimicrobial stewardship (AMS) is crucial for combating the rise of antimicrobial resistance (AMR), particularly in veterinary medicine. Educational programs targeting veterinary students can play a significant role in shaping their knowledge and attitudes toward antimicrobial use and stewardship. This study aimed to evaluate the impact of educational programs on the knowledge and attitudes regarding AMS among veterinary students in Serbia. A structured educational program on AMS was implemented for veterinary students at the Faculty of Agriculture, University of Novi Sad. Pre- and post-symposium assessments were conducted to measure changes in students’ knowledge and attitudes. The study employed a mixed-methods approach, including surveys and focus groups, to gather quantitative and qualitative data. The study resulted in significant short-term improvements in students’ knowledge of AMS principles and their attitudes toward responsible antimicrobial use. Participants demonstrated a better understanding of the mechanisms of resistance and the importance of adhering to stewardship guidelines. Qualitative feedback indicated increased awareness of the consequences of inappropriate antimicrobial use and a stronger commitment to applying stewardship practices in their future careers. The educational programs effectively enhanced immediate veterinary students’ knowledge and attitudes regarding AMS. These findings underscore the importance of integrating targeted educational programs into veterinary curricula to promote responsible antimicrobial use and combat resistance in veterinary practice.
Evolution, Ecology and Management of Wild Boar and Deer
Authors: Pérez-González Javier
Published: September 2024

Wild boar (Sus scrofa) is the most widespread member of the order Artiodactyla, a group of even-toed ungulates that are prone to overabundance, with adverse consequences for conservation, agriculture, transportation and public health [...]
Investigating the Impact of Fasting and Refeeding on Blood Biochemical Indicators and Transcriptional Profiles in the Hypothalamus and Subcutaneous Adipose Tissue in Geese
Authors: Liu Yi , Wang Xianze , Li Guangquan , Chen Shufang , Jia Huiyan , Dai Jiuli , He Daqian
Published: September 2024

Fasting and refeeding systems can cause significant short-term fluctuations in nutrient and energy levels, triggering adaptive physiological responses in animals. This study examines the effects of fasting and refeeding on blood biochemical indicators and transcriptional profiles in the hypothalamus and subcutaneous adipose tissue of geese. Biochemical assays reveal that fasting significantly increases levels of free fatty acids and glucagon, while reducing concentrations of triglycerides, leptin, and insulin. Transcriptomic analyses identify a complex transcriptional response in both the hypothalamus and subcutaneous adipose tissue, affecting several metabolic pathways and key genes associated with feed intake and energy metabolism. In subcutaneous adipose tissue, fasting downregulates genes involved in fatty acid synthesis (LPL, SCD, and ACSL1) and upregulates PLIN2, a gene promoting lipid droplet degradation. Fasting affects a variety of metabolic pathways and critical genes in the hypothalamus, including Apelin, insulin, and mTOR signaling pathways. After fasting, the mRNA expression of NOG, GABRD, and IGFBP-1 genes in the hypothalamus are significantly upregulated, while proopiomelanocortin (POMC) gene expression is markedly downregulated. This study highlights the intricate biological responses to nutritional changes in geese, which adds to our understanding of energy balance and metabolic regulation in avian species.
8-Week Kaempferia parviflora Extract Administration Improves Submaximal Exercise Capacity in Mice by Enhancing Skeletal Muscle Antioxidant Gene Expression and Plasma Antioxidant Capacity
Authors: Huang Jiapeng , Tong Yishan , Wang Shuo , Tagawa Takashi , Seki Yasuhiro , Ma Sihui , Zhang Ziwei , Cao Tiehan , Kobori Haruki , Suzuki Katsuhiko
Published: September 2024

Black ginger (Kaempferia parviflora) extract (KPE) is extracted from a ginger family plant grown in Thailand. The polyphenolic components have potential antioxidant effects and have been reported to enhance exercise performance. However, the impact of long-term KPE administration combined with long-term training on the endurance exercise performance of healthy individuals has not been fully studied. In this study, a healthy mouse model was used to investigate the effects of 8 weeks KPE administration and voluntary wheel running on the submaximal endurance exercise capacity and its mechanism. The results showed that 8 weeks of KPE administration significantly enhanced the submaximal endurance exercise capacity of mice and extended the daily voluntary wheel running distance. By measuring oxidative stress markers in plasma and the mRNA expression of antioxidant genes in skeletal muscle, we found that KPE significantly increased plasma antioxidant levels and activated the Nrf2 (Nuclear factor erythroid 2-related factor 2)/ARE (Antioxidant Response Element) pathway and its downstream antioxidant genes expression in skeletal muscle. These results suggest that KPE may enhance the antioxidant capacity of plasma and skeletal muscle by activating the Nrf2-ARE-centered antioxidant pathway, thereby increasing the daily running distance and improving the submaximal endurance exercise capacity of mice.
Quantifying the Aging of Lithium-Ion Pouch Cells Using Pressure Sensors
Authors: Nayfeh Yousof , Vittitoe Jon C. , Li Xianglin
Published: September 2024

Understanding the behavior of pressure increases in lithium-ion (Li-ion) cells is essential for prolonging the lifespan of Li-ion battery cells and minimizing the safety risks associated with cell aging. This work investigates the effects of C-rates and temperature on pressure behavior in commercial lithium cobalt oxide (LCO)/graphite pouch cells. The battery is volumetrically constrained, and the mechanical pressure response is measured using a force gauge as the battery is cycled. The effect of the C-rate (1C, 2C, and 3C) and ambient temperature (10 °C, 25 °C, and 40 °C) on the increase in battery pressure is investigated. By analyzing the change in the minimum, maximum, and pressure difference per cycle, we identify and discuss the effects of different factors (i.e., SEI layer damage, electrolyte decomposition, lithium plating) on the pressure behavior. Operating at high C-rates or low temperatures rapidly increases the residual pressure as the battery is cycled. The results suggest that lithium plating is predominantly responsible for battery expansion and pressure increase during the cycle aging of Li-ion cells rather than electrolyte decomposition. Electrochemical impedance spectroscopy (EIS) measurements can support our conclusions. Postmortem analysis of the aged cells was performed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) to confirm the occurrence of lithium plating and film growth on the anodes of the aged cells. This study demonstrates that pressure measurements can provide insights into the aging mechanisms of Li-ion batteries and can be used as a reliable predictor of battery degradation.
Sensor Fusion-Based Pulsed Controller for Low Power Solar-Charged Batteries with Experimental Tests: NiMH Battery as a Case Study
Authors: Yadasu Shyam , Awaar Vinay Kumar , Jetti Vatsala Rani , Eskandari Mohsen
Published: September 2024

Solar energy is considered the major source of clean and ubiquitous renewable energy available on various scales in electric grids. In addition, solar energy is harnessed in various electronic devices to charge the batteries and power electronic equipment. Due to its ubiquitous nature, the corresponding market for solar-charged small-scale batteries is growing fast. The most important part to make the technology feasible is a portable battery charger and the associated controllers to automate battery charging. The charger should consider the case of charging to be convenient for the user and minimize battery degradation. However, the issue of slow charging and premature battery life loss plagues current industry standards or innovative battery technologies. In this paper, a new pulse charging technique is proposed that obviates battery deterioration and minimizes the overall charging loss. The solar-powered battery charger is prototyped and executed as a practical, versatile, and compact photovoltaic charge controller at cut rates. With the aid of sensor fusion, the charge controller is disconnected and reconnects the battery during battery overcharging and deep discharging conditions using sensors with relays. The laboratory model is tested using a less expensive PV panel, battery, and digital signal processor (DSP) controller. The charging behavior of the solar-powered PWM charge controller is studied compared with that of the constant voltage–constant current (CV–CC) method. The proposed method is pertinent for minimizing energy issues in impoverished places at a reasonable price.
Therapeutic Approaches to Tuberous Sclerosis Complex: From Available Therapies to Promising Drug Targets
Authors: Conte Elena , Boccanegra Brigida , Dinoi Giorgia , Pusch Michael , De Luca Annamaria , Liantonio Antonella , Imbrici Paola
Published: September 2024

Tuberous sclerosis complex (TSC) is a rare multisystem disorder caused by heterozygous loss-of-function pathogenic variants in the tumour suppressor genes TSC1 and TSC2 encoding the tuberin and hamartin proteins, respectively. Both TSC1 and TSC2 inhibit the mammalian target of rapamycin (mTOR) complexes pathway, which is crucial for cell proliferation, growth, and differentiation, and is stimulated by various energy sources and hormonal signaling pathways. Pathogenic variants in TSC1 and TSC2 lead to mTORC1 hyperactivation, producing benign tumours in multiple organs, including the brain and kidneys, and drug-resistant epilepsy, a typical sign of TSC. Brain tumours, sudden unexpected death from epilepsy, and respiratory conditions are the three leading causes of morbidity and mortality. Even though several therapeutic options are available for the treatment of TSC, there is further need for a better understanding of the pathophysiological basis of the neurologic and other manifestations seen in TSC, and for novel therapeutic approaches. This review provides an overview of the main current therapies for TSC and discusses recent studies highlighting the repurposing of approved drugs and the emerging role of novel targets for future drug design.
Targeting CD47 and Angiogenesis Demonstrates Effective Anti-Tumor Effect in Bladder Cancer
Authors: Huang Xiting , Wang Qian , Nan Yanyang , Zhang Xuyao , Xu Ke , Ju Dianwen , Ding Weihong
Published: September 2024

Background: Although immunotherapy has shown potential in cancer treatment, current immunotherapeutics for bladder cancer are limited by a low response rate. Therefore, it is necessary to investigate other suitable immunotherapeutic targets and strategies for bladder cancer. Methods: To evaluate whether CD47 could be a suitable target for bladder cancer immunotherapy, CD47 protein expression levels in 116 bladder cancer tissue samples were assessed by IHC staining. In vitro anti-tumor effect of blocking CD47 was examined by phagocytosis assays. In vivo anti-tumor effects of targeting CD47 and angiogenesis were experimented in the HSPCs-CDX model. Results: We find that CD47 is highly expressed in bladder cancer samples and is associated with poor prognosis. Blocking CD47 could enhance the human PBMC-derived macrophages’ phagocytosis of T24 (from 10.40% to 29.70%) and 5637 (from 5.31% to 33.52%) human bladder cancer cells, as well as demonstrate anti-tumor effects in the HSPCs-CDX model (tumor growth inhibition rate, TGI: 33.05%). During CD47 treatment, we observed that the level of angiogenesis increased after CD47 blockade, and it might undermine the effect of CD47 immunotherapy. We then combined CD47 blockade with anti-angiogenic drugs to treat bladder cancer and discovered that inhibiting angiogenesis could further improve the anti-tumor effect of CD47 blockade (TGI: 76.39%). Finally, we tested the anti-tumor effect of co-targeting CD47 and angiogenesis using a bispecific fusion protein, SIRPα-VEGFR1, which successfully inhibited tumor growth to a similar extent as a combination therapy. Conclusions: Our study suggests that targeting CD47 could inhibit the growth of bladder cancer by promoting macrophage-mediated anti-tumor immunity. Moreover, blocking CD47 and angiogenesis could achieve a potent anti-tumor effect and could be an effective immunotherapy strategy for bladder cancer.
Numerical Study on Mechanical Characteristics of Tower Sections with Main Member Disconnection
Authors: Zheng Hengwei , Wu Changli , Liu Jinhong , Zhong Lang , Li Kai , Yan Zhitao
Published: September 2024

Restricted by the existing construction technology, there are a lot of disconnections in the angle steel components of transmission towers. At present, there are more studies on single angle steel or cross bracing, but less on the main member containing disconnection joints. According to the disconnection position in the main member, an upper end disconnection, middle end disconnection, and lower end disconnection were designed in this paper. At the same time, a tower section model of a connected main member and a tower section model of a disconnected main member were established and analyzed by finite element analysis. Considering the loadings acting on transmission towers, the two load conditions of axial loading and tension–compression coupling are set. Considering the loadings acting on transmission towers, the influences of the combination form of the inner and outer steel cladding and the steel cladding area ratio on the ultimate bearing capacity of the main member were studied under 72 groups of different tower sections applied with axial loadings. The influence of the disconnection joints on the stability of the tower section was studied under 24 groups of different tower sections applied with tension and compression coupled loading conditions. Referring to the specifications, the slenderness ratio correction factor formula of the disconnect main member can be derived. The results indicate that when designing the disconnection joint in the main member, it is recommended to choose a middle end disconnection with a steel cladding area ratio of 1.0.
20th-Century World Built Heritage Facing Water: Conservation of Fallingwater and Boa Nova Tea House
Authors: Milão Susana , Ribeiro Telma , Neves Isabel Clara , Lima Ana , Pacheco Luís Paulo
Published: September 2024

The conservation of 20th-century architectural heritage presents unique challenges due to the modern materials and construction techniques used by pioneering architects. This paper investigates the preservation challenges of iconic architectural works exposed to water environments, focusing on Fallingwater by Frank Lloyd Wright and the Boa Nova Tea House by Álvaro Siza Vieira. These structures, situated in natural settings with significant exposure to water, serve as case studies for examining conservation methods and their broader implications. This study explores archival documents, interviews with conservation experts, and literature reviews to highlight the main degradation phenomena related to the impact of environmental conditions on the material’s integrity. Despite limited specific data on monitoring and maintenance, this research underscores the importance of ongoing conservation efforts. Fallingwater’s case highlights the necessity of restoration techniques, while the Boa Nova Tea House illustrates minimal intervention strategies to maintain authenticity. This study concludes that the effective conservation of 20th-century built heritage necessitates a balance between preserving original design intents and adapting to environmental challenges. It emphasizes the need for innovative conservation strategies, adherence to international guidelines, and the establishment of comprehensive management and monitoring plans to safeguard the integrity and authenticity of culturally significant architecture.
Energy Flexibility Strategies for Buildings in Hot Climates: A Case Study for Dubai
Authors: Saberi-Derakhtenjani Ali , Barbosa Juan David , Rodriguez-Ubinas Edwin
Published: September 2024

This paper presents a case study of energy flexibility strategies for a building located in the hot climate of Dubai, a type of climate in which energy flexibility has been under-researched. Energy flexibility is changing the routine-consumption profile and deviating from the normal operation of the building by the users to manage the variability in the load profile and cost of electricity. The three flexibility strategies being considered are based on the modulation of the indoor air temperature setpoint profile while considering different marginal costs for electricity. The main objective is to quantify the energy storage flexibility of each strategy and evaluate its impact on the system ramping and load factor. The study was carried out utilizing a grey-box, resistance–capacitance model of the building, which was validated against experimental measurements. This study is the first to use the following five indicators simultaneously: load factor, system ramping, storage capacity, peak-period demand reduction, and cost savings. Combining these indicators helps building facility managers and distribution system operators (DSOs) better understand the implications of implementing a specific flexibility strategy in a building or a group of buildings. When comparing the indicators of each strategy with each other, it was observed that depending on the amplitude of the change in the electricity cost signal during the peak period, a significant cost reduction of more than 25% could be achieved through the implementation of specific flexibility strategies compared with the normal baseline operating condition.
Enhancing Knowledge on Energy Refurbishment of Buildings and Green Procurement through Living Labs
Authors: Marksel Maršenka , Pavletič Nina , Letnik Tomislav
Published: September 2024

Buildings account for a significant portion of energy consumption and greenhouse gas emissions, underscoring the urgent need for energy refurbishment and green procurement strategies. This study explores the potential of Living Labs, a collaborative and user-centered approach, to bridge knowledge gaps and foster innovation in these areas. The research employed a comprehensive methodology, including stakeholder surveys and statistical analyses, to evaluate knowledge enhancement in various domains such as green policies, mentoring, funding possibilities, and green public procurement. The results demonstrated statistically significant improvements in knowledge across different fields and stakeholder groups. This study contributes to the objectives of the European Green Deal by highlighting the role of Living Labs in advancing knowledge critical to achieving energy efficiency and sustainability targets. Based on these findings, several recommendations are proposed to further leverage the effectiveness of Living Labs in promoting the energy refurbishment of buildings and green public procurement.
Assessing Factors Affecting Fall Accidents among Hispanic Construction Workers: Integrating Safety Insights into BIM for Enhanced Life Cycle Management
Authors: Kayastha Rujan , Kisi Krishna
Published: September 2024

Falls are the most common type of accident in the construction industry, and falls to a lower level are among the leading causes of fatalities. Work-related fatalities due to falls, slips, and trips have been increasing, with Hispanic workers among the highest fatalities. This study investigated the association between fall accidents and attributes such as age, musculoskeletal pain (MSPs), sleep hours, safety knowledge, use of personal protective equipment (PPE), and working hours among Hispanic construction workers involved in building construction. This study collected 220 valid responses and used nonparametric chi-square tests and binary logistic regression to analyze the data. This study found that the location of the fall, MSPs, and use of personal protective equipment have a significant effect on the likelihood of having fall accidents. The strongest predictor of fall accidents was “fall from a ladder”, followed by having two or three MSPs. The use of PPE had the highest decreasing ratio in odds of fall accidents, indicating the importance of wearing PPE properly. The results show the importance of integrating safety management strategies within construction projects’ broader life cycle management. The insights list how project engineers can incorporate these findings into Building Information Modeling (BIM) systems to enhance project planning and safety measures in reducing fall-related accidents and their severe consequences. This study highlights the importance of addressing MSPs, properly using PPE, and reducing falls from ladders in the construction industry to prevent fall accidents among Hispanic workers and minimize their severe consequences.
Impact of Shield Tunnel Construction on Adjacent Railway Bridge: Protective Measures and Deformation Control
Authors: Liu Wen , Zhao Lu , Yao Xiang-Chuan , Zheng Hai-Ao , Liu Wen-Li
Published: September 2024

With the rapid development of urban rail transit networks, constructing shield tunnels often requires passing underneath existing buildings, which can potentially impact their safety. This study examined the impact of constructing a double-line shield tunnel underneath a railway bridge on the adjacent pile foundation via numerical simulation. Protective measures, including construction parameter control, grouting methods, monitoring, and early warning systems, were implemented to mitigate impacts. The results indicated that the bridge deformation fell within acceptable limits, with maximum horizontal and longitudinal displacements of 0.06 mm and a maximum vertical displacement of −0.31 mm. The railway bridge pile foundation experienced maximum horizontal and longitudinal displacements of 0.47 mm and vertical displacements of −0.23 mm during construction. Enhanced construction quality control and monitoring effectively controlled deformation to ensure the railway safety. This study provides valuable guidance for similar projects and future urban rail transit developments.
Are Dual-Phase 18F-Fluorodeoxyglucose PET-mpMRI Diagnostic Performances to Distinguish Brain Tumour Radionecrosis/Recurrence after Cranial Radiotherapy Usable in Routine?
Authors: Cailleteau Axel , Ferrer Ludovic , Geffroy Delphine , Fleury Vincent , Lalire Paul , Doré Mélanie , Rousseau Caroline
Published: September 2024

Brain metastases or primary brain tumours had poor prognosis until the use of high dose radiotherapy. However, radionecrosis is a complex challenge in the post-radiotherapy management of these patients due to the difficulty of distinguishing this complication from local tumour recurrence. MRI alone has a variable specificity and sensibility, as does PET-CT imaging. We aimed to investigate the diagnostic performance of dual-phase 18F-FDG PET-mpMRI to distinguish cerebral radionecrosis from local tumour recurrence after cranial radiotherapy. A retrospective analysis was conducted between May 2021 and September 2022. Inclusion criteria encompassed patients with inconclusive MRI findings post-radiotherapy and history of cerebral radiotherapy for primary or metastatic brain lesions. Lesions are assessed qualitatively and semi-quantitatively. The gold standard to assess radionecrosis was histopathology or a composite criterion at three months. The study evaluated 24 lesions in 23 patients. Qualitative analysis yielded 85.7% sensitivity and 75% specificity. Semi-quantitative analysis, based on contralateral background noise, achieved 100% sensitivity and 50% specificity. Moreover, using contralateral frontal lobe background noise resulted in higher performances with 92% sensitivity and 63% specificity. Stratification by lesion type demonstrated 100% sensitivity and specificity rates for metastatic lesions. The diagnostic performance of dual-phase 18F-FDG PET-mpMRI shows promising results for metastatic lesions.