bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Downhill Running and Maturation on Histological and Morphological Properties of Tendon and Enthesis in Mice
Authors: Ozone Kaichi , Minegishi Yuki , Oka Yuichiro , Sato Michiaki , Kanemura Naohiko
Published: March 2023

To date, it remains unclear how overuse affects the tendons and entheses at different stages of maturation. Therefore, we evaluated histological and morphological changes in the tendons and entheses in adolescent (4-week-old) and adult mice (8-week-old) by performing flat-land and downhill running exercises. The mice were divided into the Sedentary, High Flat (flat-land high-speed running; concentric-contraction exercise), Low Down (downhill low-speed running; eccentric-contraction exercise), and High Down (downhill high-speed running; eccentric-contraction exercise) groups. Histological changes and inflammatory factor expressions were compared in the entheses and tendons after 4 weeks of exercise. Downhill, but not flat-land high-speed running, induced muscle–tendon complex hypertrophy in both adolescent and adult mice. Histological enthesis changes were induced in both groups during downhill running but were less pronounced in adult mice. Conversely, no significant cell aggregation or fiber orientation changes were observed in the tendon, but increased inflammatory factors were observed in both groups, with significantly higher expression in the tendons of adult mice. Downhill running induced histological and morphological enthesis changes and inflammatory factor increase in the tendons, regardless of running speed variations. These results may help elucidate the pathogenesis of enthesopathy and tendinopathy, which have different pathophysiologies despite having the same pathogenetic factors.
Potential Impact of Polymorphisms in Toll-like Receptors 2, 3, 4, 7, 9, miR-146a, miR-155, and miR-196a Genes on Osteoarthritis Susceptibility
Authors: Stefik Debora , Vranic Vladimir , Ivkovic Nemanja , Velikic Gordana , Maric Dusan M. , Abazovic Dzihan , Vojvodic Danilo , Maric Dusica L. , Supic Gordana
Published: March 2023

Osteoarthritis (OA) is a progressive inflammatory disease of synovial joints and a leading cause of disability among adults. Inflammation-related genes, including genes for Toll-like receptors (TLRs), are tightly controlled by several microRNAs that, in addition to their pivotal role in the epigenetic regulation of target genes, are ligands for TLR activation and downstream signaling. Thus, we evaluated the association between OA risk and genetic variants in TLR2, TLR3, TLR4, TLR7, TLR9, and microRNAs that regulate TLRs signaling miR146a, miR155, and miR196a2. Our study group consisted of 95 surgically treated OA patients and a control group of 104 healthy individuals. Genetic polymorphisms were determined using TaqMan real-time PCR assays (Applied Biosystems). Adjusted logistic regression analysis demonstrated that polymorphisms in TLR4 rs4986790 (OR = 2.964, p = 0.006), TLR4 rs4986791 (OR = 8.766, p = 0.00001), and TLR7 rs385389 (OR = 1.579, p = 0.012) increased OA risk, while miR-196a2 rs11614913 (OR = 0.619, p = 0.034) was significantly associated with decreased OA risk. Our findings indicate that polymorphisms in the TLR4 and TLR7 genes might increase OA risk and suggest a novel association of miR-196a2 polymorphism with decreased OA susceptibility. The modulation of TLRs and miRNAs and their cross-talk might be an attractive target for a personalized approach to OA management.
Exosomal Non-Coding RNAs: Novel Regulators of Macrophage-Linked Intercellular Communication in Lung Cancer and Inflammatory Lung Diseases
Authors: Lai Xingning , Zhong Jie , Zhang Boyi , Zhu Tao , Liao Ren
Published: March 2023

Macrophages are innate immune cells and often classified as M1 macrophages (pro-inflammatory states) and M2 macrophages (anti-inflammatory states). Exosomes are cell-derived nanovesicles that range in diameter from 30 to 150 nm. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), are abundant in exosomes and exosomal ncRNAs influence immune responses. Exosomal ncRNAs control macrophage-linked intercellular communication via their targets or signaling pathways, which can play positive or negative roles in lung cancer and inflammatory lung disorders, including acute lung injury (ALI), asthma, and pulmonary fibrosis. In lung cancer, exosomal ncRNAs mediated intercellular communication between lung tumor cells and tumor-associated macrophages (TAMs), coordinating cancer proliferation, migration, invasion, metastasis, immune evasion, and therapy resistance. In inflammatory lung illnesses, exosomal ncRNAs mediate macrophage activation and inflammation to promote or inhibit lung damage. Furthermore, we also discussed the possible applications of exosomal ncRNA-based therapies for lung disorders.
Unlocking the Pragmatic Potential of Regenerative Therapies in Heart Failure with Next-Generation Treatments
Authors: Kishino Yoshikazu , Fukuda Keiichi
Published: March 2023

Patients with chronic heart failure (HF) have a poor prognosis due to irreversible impairment of left ventricular function, with 5-year survival rates <60%. Despite advances in conventional medicines for HF, prognosis remains poor, and there is a need to improve treatment further. Cell-based therapies to restore the myocardium offer a pragmatic approach that provides hope for the treatment of HF. Although first-generation cell-based therapies using multipotent cells (bone marrow-derived mononuclear cells, mesenchymal stem cells, adipose-derived regenerative cells, and c-kit-positive cardiac cells) demonstrated safety in preclinical models of HF, poor engraftment rates, and a limited ability to form mature cardiomyocytes (CMs) and to couple electrically with existing CMs, meant that improvements in cardiac function in double-blind clinical trials were limited and largely attributable to paracrine effects. The next generation of stem cell therapies uses CMs derived from human embryonic stem cells or, increasingly, from human-induced pluripotent stem cells (hiPSCs). These cell therapies have shown the ability to engraft more successfully and improve electromechanical function of the heart in preclinical studies, including in non-human primates. Advances in cell culture and delivery techniques promise to further improve the engraftment and integration of hiPSC-derived CMs (hiPSC-CMs), while the use of metabolic selection to eliminate undifferentiated cells will help minimize the risk of teratomas. Clinical trials of allogeneic hiPSC-CMs in HF are now ongoing, providing hope for vast numbers of patients with few other options available.
Immunization with Neural-Derived Peptides in Neurodegenerative Diseases: A Narrative Review
Authors: Monroy Germán Rivera , Murguiondo Pérez Renata , Weintraub Ben Zión Efraín , Vidal Alcántar-Garibay Oscar , Loza-López Ericka Cristina , Tejerina Marion Emilio , Blancarte Hernández Enrique , Navarro-Torres Lisset , Ibarra Antonio
Published: March 2023

Neurodegenerative diseases (NDDs) are a major health problem worldwide. Statistics suggest that in America in 2030 there will be more than 12 million people suffering from a neurodegenerative pathology. Furthermore, the increase in life expectancy enhances the importance of finding new and better therapies for these pathologies. NDDs could be classified into chronic or acute, depending on the time required for the development of clinical symptoms and brain degeneration. Nevertheless, both chronic and acute stages share a common immune and inflammatory pathway in their pathophysiology. Immunization with neural-derived peptides (INDP) is a novel therapy that has been studied during the last decade. By inoculating neural-derived peptides obtained from the central nervous system (CNS), this therapy aims to boost protective autoimmunity, an autoreactive response that leads to a protective phenotype that produces a healing environment and neuroregeneration instead of causing damage. INDP has shown promising findings in studies performed either in vitro, in vivo or even in some pre-clinical trials of different NDDs, standing as a potentially beneficial therapy. In this review, we will describe some of the studies in which the effect of INDP strategies have been explored in different (chronic and acute) neurodegenerative diseases.
Developing a Fluorescent Inducible System for Free Fucose Quantification in Escherichia coli
Authors: Nuñez Samantha , Barra Maria , Garrido Daniel
Published: March 2023

L-Fucose is a monosaccharide abundant in mammalian glycoconjugates. In humans, fucose can be found in human milk oligosaccharides (HMOs), mucins, and glycoproteins in the intestinal epithelium. The bacterial consumption of fucose and fucosylated HMOs is critical in the gut microbiome assembly of infants, dominated by Bifidobacterium. Fucose metabolism is important for the production of short-chain fatty acids and is involved in cross-feeding microbial interactions. Methods for assessing fucose concentrations in complex media are lacking. Here we designed and developed a molecular quantification method of free fucose using fluorescent Escherichia coli. For this, low- and high-copy plasmids were evaluated with and without the transcription factor fucR and its respective fucose-inducible promoter controlling the reporter gene sfGFP. E. coli BL21 transformed with a high copy plasmid containing pFuc and fucR displayed a high resolution across increasing fucose concentrations and high fluorescence/OD values after 18 h. The molecular circuit was specific against other monosaccharides and showed a linear response in the 0–45 mM range. Adjusting data to the Hill equation suggested non-cooperative, simple regulation of FucR to its promoter. Finally, the biosensor was tested on different concentrations of free fucose and the supernatant of Bifidobacterium bifidum JCM 1254 supplemented with 2-fucosyl lactose, indicating the applicability of the method in detecting free fucose. In conclusion, a bacterial biosensor of fucose was validated with good sensitivity and precision. A biological method for quantifying fucose could be useful for nutraceutical and microbiological applications, as well as molecular diagnostics.
Prefrontal Cortex Hemodynamics and Functional Connectivity Changes during Performance Working Memory Tasks in Older Adults with Sleep Disorders
Authors: Gao Jiahui , Zhang Lin , Zhu Jingfang , Guo Zhenxing , Lin Miaoran , Bai Linxin , Zheng Peiyun , Liu Weilin , Huang Jia , Liu Zhizhen
Published: March 2023

Objective: Older adults with sleep disorders (SDs) show impaired working memory abilities, and working memory processes are closely related to the prefrontal cortex (PFC). However, the neural mechanism of working memory impairment in older adults with SD remains unclear. This study aimed to investigate changes in PFC function among older adults with SD when carrying out the N-back task by functional near-infrared spectroscopy (fNIRS). Method: A total of 37 older adults with SDs were enrolled in this study and matched with 37 healthy older adults by gender, age, and years of education. Changes in PFC function were observed by fNIRS when carrying out the N-back task. Results: The accuracy on the 0-back and 2-back tasks in the SD group was significantly lower than that in the healthy controls (HC) group. The oxygenated hemoglobin (oxy-Hb) concentration of channel 8 which located in the dorsolateral prefrontal cortex (DLPFC) was significantly reduced in the SD group during the 2-back task, and the channel-to-channel connectivity between the PFC subregions was significantly decreased. Conclusions: These results suggest that patients with sleep disorders have a weak performance of working memory; indeed, the activation and functional connectivity in the prefrontal subregions were reduced in this study. This may provide new evidence for working memory impairment and brain function changes in elderly SDs.
Among Gerontogens, Heavy Metals Are a Class of Their Own: A Review of the Evidence for Cellular Senescence
Authors: Vielee Samuel T. , Wise John P.
Published: March 2023

Advancements in modern medicine have improved the quality of life across the globe and increased the average lifespan of our population by multiple decades. Current estimates predict by 2030, 12% of the global population will reach a geriatric age and live another 3–4 decades. This swelling geriatric population will place critical stress on healthcare infrastructures due to accompanying increases in age-related diseases and comorbidities. While much research focused on long-lived individuals seeks to answer questions regarding how to age healthier, there is a deficit in research investigating what aspects of our lives accelerate or exacerbate aging. In particular, heavy metals are recognized as a significant threat to human health with links to a plethora of age-related diseases, and have widespread human exposures from occupational, medical, or environmental settings. We believe heavy metals ought to be classified as a class of gerontogens (i.e., chemicals that accelerate biological aging in cells and tissues). Gerontogens may be best studied through their effects on the “Hallmarks of Aging”, nine physiological hallmarks demonstrated to occur in aged cells, tissues, and bodies. Evidence suggests that cellular senescence—a permanent growth arrest in cells—is one of the most pertinent hallmarks of aging and is a useful indicator of aging in tissues. Here, we discuss the roles of heavy metals in brain aging. We briefly discuss brain aging in general, then expand upon observations for heavy metals contributing to age-related neurodegenerative disorders. We particularly emphasize the roles and observations of cellular senescence in neurodegenerative diseases. Finally, we discuss the observations for heavy metals inducing cellular senescence. The glaring lack of knowledge about gerontogens and gerontogenic mechanisms necessitates greater research in the field, especially in the context of the global aging crisis.
A Psychometric Study of the Prosocial Behavior Scale: Differential Item Functioning by Gender
Authors: Martínez-Gregorio Sara , Tomás José M. , Oliver Amparo
Published: March 2023

Some scales co-exist in the literature to measure prosocial behavior in adolescents. Gender differences in prosocial behavior have been a controversial topic of research. To strengthen future research in the area, the psychometric properties of the most used instruments must be guaranteed, especially its gender non-bias. Our study provides psychometric evidence for the Prosocial Behavior Scale in a sample of adolescents, exploring: (a) its factor structure; (b) reliability; (c) gender-related differential item functioning (DIF); (d) nomological validity. A sample of 512 high school students (mean age = 13.62 (SD = 1.34), 51.6% females) participated in the research. Confirmatory Factor Analysis (CFA) was used to test the factor structure of the scale, which adequately fitted the data (χ2 (35) = 152.224, p < 0.001, Comparative Fit Index (CFI) = 0.905, Root Mean Squared Error of Approximation (RMSEA) = 0.085 90%CI [0.072–0.099], Standardized Root Mean Squared Residual (SRMR) = 0.079). Reliability results were good (α = 0.74, ω = 0.74). Regarding the DIF, five items presented some gender-related bias, generally benefiting females. However, the DIF impact could be considered negligible. Correlations with the subdimensions of the psychological capital offered evidence of the nomological validity of the scale. In conclusion, the scale presented adequate psychometric properties that support its ability to effectively assess prosocial behavior and gender differences in the prosocial behavior samples of adolescents. Additionally, the results obtained imply that gender differences in the manifestations of prosocial behavior require measurements that can fairly sample behaviors characteristic of each gender.
New Times, New Ways: Exploring the Self-Regulation of Sport during the COVID-19 Pandemic and Its Relationship with Nostalgia and Well-Being
Authors: Cho Heetae , Chen Mun Yip Kinnard , Kang Hyoung-Kil , Chiu Weisheng
Published: March 2023

Coronavirus disease (COVID-19) has negatively affected individuals’ participation in sport activities, while sport participation is an important regulator of well-being. The current study investigated the effects of the nostalgia for sport activities and self-regulation of sport activities on subjective well-being. A total of 302 responses were collected from participants who had engaged in sport activities before the lockdown period. The data were analyzed using partial least squares structural equation modeling (PLS-SEM). The findings showed that nostalgia positively affected the self-regulation of sport and subjective well-being. In addition, self-regulation of sport was positively associated with subjective well-being. Based on the findings of this study, policymakers can implement interventions that promote an individual’s feelings of nostalgia, as it might lead them to engage in sport or promote self-regulation.
Uplift Behaviour of External Fibre-Reinforced Polymer Wrapping on RC Piles in Dry and Submerged Sandy Soil
Authors: Meeran Mydeen Mohamed Younus , Madasamy Murugan , Seeni Bright Singh
Published: March 2023

The sudden occurrence of an earthquake induces a liquefaction effect on foundation soil, which causes a substantial increase in the uplift pressure acting on piles and causes structural damage to superstructures. This forms the basis of the necessity of experimenting with the behaviour of piles subjected to uplift loads and predicting their load-carrying capacity or resistance. Fibre-reinforced polymer (FRP) wraps are widely used for strengthening and retrofitting piles subjected to damage. The current study is aimed at determining the uplift load-carrying capacity or resistance of piles wrapped with basalt fibre-reinforced polymer (BFRP) and glass fibre-reinforced polymer (GFRP) sheets by experiment. Preliminary tests were conducted to identify the influence of BFRP and GFRP wraps on the mechanical strength properties of concrete. The mechanical strength of the specimen with the double wrapping of basalt and glass fibres in the perpendicular direction outperformed all other specimens. Moreover, the piles were wrapped with laminates and experimented on for their uplift capacity in dry and submerged conditions. The results indicate a considerable improvement in the uplift resistance of the piles compared with the unconfined piles. The BFRP and GFRP wraps improved the uplift resistance of the piles by 35.56% and 15.56%, respectively, higher than the unconfined pile for dry conditions. The angle of the interfacial friction in dry and submerged states was observed to be the maximum for the perpendicular direction for both of the FRP wraps, and the failure modes were compared. The simulated model showed a significant correctness for determining the uplift resistance of FRP-wrapped piles in dry and submerged states. The degree of agreement in the dry condition for the experimental results and finite element method was more than 94% for all fibre wraps.
Ground Improvement by Construction and Demolition Waste (CDW) Soil Mixture Replacement
Authors: Hidalgo Cesar , Carvajal Gloria , Hincapie Angela , Muñoz Fredy , Hernández Mario
Published: March 2023

In several countries, brick and ceramic tile are the most important construction materials; therefore, associated waste generation is common in construction and demolitions. An alternative use for waste is to incorporate it into road construction. However, the biggest limitation to use it as structural pavement layers is that strength and durability regulatory requirements are not met for highways when it is used. As an alternative, construction and demolition waste (CDW) soil mixtures are proposed as subgrade improvements which require less of a thickness increase of pavement structures to meet highway standards. The results of this article present the behavior of silty soil, brick residues, and ceramic tile mixtures in different added material ratios. Laboratory evaluations were conducted and included material characterization, compaction tests, obtaining CBR values, and obtaining resilient moduli. A parametric thicknesses evaluation was performed on flexible pavement structures with different traffic conditions and CDW ratios. It was concluded that CDW material addition increases strength and the resilient modulus similarly to granular subbase (AASHTO M147-65). Therefore, the pavement thickness can be reduced, and costs can be decreased by more than 7%.
Optimization and Process Effect for Microalgae Carbon Dioxide Fixation Technology Applications Based on Carbon Capture: A Comprehensive Review
Authors: Li Gang , Xiao Wenbo , Yang Tenglun , Lyu Tao
Published: March 2023

Microalgae carbon dioxide (CO2) fixation technology is among the effective ways of environmental protection and resource utilization, which can be combined with treatment of wastewater and flue gas, preparation of biofuels and other technologies, with high economic benefits. However, in industrial application, microalgae still have problems such as poor photosynthetic efficiency, high input cost and large capital investment. The technology of microalgae energy development and resource utilization needs to be further studied. Therefore, this work reviewed the mechanism of CO2 fixation in microalgae. Improving the carbon sequestration capacity of microalgae by adjusting the parameters of their growth conditions (e.g., light, temperature, pH, nutrient elements, and CO2 concentration) was briefly discussed. The strategies of random mutagenesis, adaptive laboratory evolution and genetic engineering were evaluated to screen microalgae with a high growth rate, strong tolerance, high CO2 fixation efficiency and biomass. In addition, in order to better realize the industrialization of microalgae CO2 fixation technology, the feasibility of combining flue gas and wastewater treatment and utilizing high-value-added products was analyzed. Considering the current challenges of microalgae CO2 fixation technology, the application of microalgae CO2 fixation technology in the above aspects is expected to establish a more optimized mechanism of microalgae carbon sequestration in the future. At the same time, it provides a solid foundation and a favorable basis for fully implementing sustainable development, steadily promoting the carbon peak and carbon neutrality, and realizing clean, green, low-carbon and efficient utilization of energy.
The BAFF-APRIL System in Cancer
Authors: Ullah Md Ashik , Mackay Fabienne
Published: March 2023

B cell-activating factor (BAFF; also known as CD257, TNFSF13B, BLyS) and a proliferation-inducing ligand (APRIL; also known as CD256, TNFSF13) belong to the tumor necrosis factor (TNF) family. BAFF was initially discovered as a B-cell survival factor, whereas APRIL was first identified as a protein highly expressed in various cancers. These discoveries were followed by over two decades of extensive research effort, which identified overlapping signaling cascades between BAFF and APRIL, controlling immune homeostasis in health and driving pathogenesis in autoimmunity and cancer, the latter being the focus of this review. High levels of BAFF, APRIL, and their receptors have been detected in different cancers and found to be associated with disease severity and treatment response. Here, we have summarized the role of the BAFF-APRIL system in immune cell differentiation and immune tolerance and detailed its pathogenic functions in hematological and solid cancers. We also highlight the emerging therapeutics targeting the BAFF-APRIL system in different cancer types.
Polydopamine-Coated Polyurethane Foam as a Structured Support for the Development of an Easily Reusable Heterogeneous Photocatalyst Based on Eosin Y
Authors: Peng Han , Romero Thierry , Bertani Philippe , Ritleng Vincent
Published: March 2023

An easy-to-handle eosin Y-based heterogeneous photocatalyst was prepared by post-functionalization of a polydopamine-coated open cell polyurethane foam (PDA@PUF) via the silanization of the adhesive layer with 3-(triethoxysilyl)propan-1-amine (APTES) and the subsequent EDC-mediated coupling of the resulting amino-functionalized foam with eosin Y. The obtained macroscopic material, EY-APTES@PDA@PUF, showed good efficiency and excellent reusability, in an easy-to-carry “dip-and-play” mode for at least six runs as photocatalyst for the aerobic oxidation of 2-methyl-5-nitroisoquinolin-2-ium iodide to the corresponding isoquinolone. Subsequent investigation of the catalytic efficiency of EY-APTES@PDA@PUF for the oxidation of sulfides to sulfoxides, however, evidenced non-negligible eosin Y leaching, leading to a progressive deactivation of the catalytic foam in this case. Two alternative synthetic protocols for the preparation of the macroscopic photocatalyst were next explored to avoid eosin Y leaching. In both cases however, cycling tests also highlighted a progressive deactivation of the catalytic foams in sulfide-to-sulfoxide oxidation reactions.
Molecular Analysis of the Superior Efficacy of a Dual Epidermal Growth Factor Receptor (EGFR)-DNA-Targeting Combi-Molecule in Comparison with Its Putative Prodrugs 6-Mono-Alkylamino- and 6,6-Dialkylaminoquinazoline in a Human Osteosarcoma Xenograft Model
Authors: Facchin Caterina , Fraga-Timiraos Ana B. , Schmitt Julie , Babaa Nadia , Pannu Naveet , Aliaga Antonio , Larroque Anne-Laure , Jean-Claude Bertrand J.
Published: March 2023

Background: ZR2002 is a dual EGFR-DNA-targeting combi-molecule that carries a chloroethyl group at the six-position of the quinazoline ring designed to alkylate DNA. Despite its good pharmacokinetics, ZR2002 is metabolized in vivo into dechlorinated metabolites, losing the DNA-alkylating function required to damage DNA. To increase the DNA damage activity in tumor cells in vivo, we compared ZR2002 with two of its 6-N,N-disubstituted analogs: “JS61”, with a nitrogen mustard function at the six-position of the quinazoline ring, and “JS84”, with an N-methyl group. Methods: Tumor xenografts were performed with the human Saos-2 osteosarcoma cell line expressing EGFR. Mice were treated with ZR2002, JS84 or JS61, and the tumor burden was measured with a caliper and CT/PET imaging. Drug metabolism was analyzed with LC-MS. EGFR and ɣ-H2AX phosphorylation were quantified via Western blot analysis and immunohistochemistry. Results: In vivo analysis showed that significant tumor growth inhibition was only achieved when ZR2002 was administered in its naked form. The metabolic dealkylation of JS61 and JS84 did not release sufficient concentrations of ZR2002 for the intratumoral inhibition of P-EGFR or enhanced levels of P-H2AX. Conclusions: The results in toto suggest that intratumoral concentrations of intact ZR2002 are correlated with the highest inhibition of P-EGFR and induction of DNA damage in vivo. ZR2002 may well represent a good drug candidate for the treatment of EGFR-expressing osteosarcoma.
Carbon Dot-Functionalized Solution-Gated Graphene Transistors for Highly Sensitive Detection of Cobalt(II) Ions
Authors: Ren Zhanpeng , Wang Jianying , Xue Chenglong , Deng Minghua , Li Ziqin , Zhang Huibin , Cai Chen , Xu Bing , Wang Xianbao , Li Jinhua
Published: March 2023

A carbon dot-functionalized solution-gated graphene transistor (CD-SGGT) was designed and prepared via the modification of CDs on the gate of SGGT. The above CDs were hydrothermally synthesized using DL-thioctic acid and triethylenetramine as C, N and S sources. The average size of CDs was ~6.2 nm, and there were many amino and carboxyl groups on the CDs’ surfaces. The CDs was then used as a probe for preparation of CD-SGGT sensor for the cobalt(II) (Co2+) ions detection. The CD-SGGT sensor showed excellent sensitivity and high selectivity. Remarkably, the limit of detection (LOD) reached 10−19 M. The linear detection range was obtained from 10−19 to 10−15 M. Additionally, the CD-SGGT also showed fast response and good stability.
Experimental-Theoretical Approach for the Chemical Detection of Glyphosate and Its Potential Interferents Using a Copper Complex Fluorescent Probe
Authors: Martins Guilherme , Oliveira Karolyne , Weheabby Saddam , Al-Hamry Ammar , Kanoun Olfa , Rüffer Tobias , Cabral Benedito , Paterno Leonardo
Published: March 2023

The present contribution proposes an optical method for the detection of glyphosate (GLY) using a Cu(II) bis-(oxamate) complex ([Cu(opba)]2−) as the fluorescent probe. It wa found that in acetonitrile solution, its fluorescence increases in the presence of GLY and scales linearly (R2 = 0.99) with GLY concentration in the range of 0.7 to 5.5 µM, which is far below that established by different international regulations. The probe is also selective to GLY in the presence of potential interferents, namely aminomethyl phosphonic acid and N-nitrosoglyphosate. Theoretical results obtained by time-dependent density functional theory coupled to a simplified treatment of the liquid environment by using a self-consistent reaction-field revealed that GLY molecules do not coordinate with the central Cu2+ ion of [Cu(opba)]2−; instead, they interact with its peripheral ligand through hydrogen bond formation. Thereby, GLY plays mainly the role of the proton donor. The results also suggest that GLY increases the dielectric constant of the medium when it contributes to the stabilization of the excited state of the [Cu(opba)]2− and enhancement of its fluorescence.
Relationship between Birth Order and Postnatal Growth until 4 Years of Age: The Japan Environment and Children’s Study
Authors: Yoshida Aya , Kaneko Kayo , Aoyama Kohei , Yamaguchi Naoya , Suzuki Atsushi , Kato Sayaka , Ebara Takeshi , Sugiura-Ogasawara Mayumi , Kamijima Michihiro , Saitoh Shinji ,   the Japan Environment and Children’s Study Group
Published: March 2023

Later-borns tend to be shorter than first-borns in childhood and adulthood. However, large-scale prospective studies examining growth during infancy according to birth order are limited. We aimed to investigate the relationship between birth order and growth during the first 4 years of life in a Japanese prospective birth cohort study. A total of 26,249 full-term singleton births were targeted. General linear and multivariable logistic regression models were performed and adjusted for birth weight, parents’ heights, maternal age at delivery, gestational weight gain, maternal smoking and alcohol drinking status during pregnancy, household income, breastfeeding status, and Study Areas. The multivariate adjusted mean length Z-scores in “first-borns having no sibling”, “first-borns having siblings”, “second-borns”, and “third-borns or more” were −0.026, −0.013, 0.136, and 0.120 at birth and −0.324, −0.330, −0.466, and −0.569 at 10 months, respectively. Results similar to those at 10 months were observed at 1.5, 3, and 4 years. The adjusted odds ratios (95% confidence intervals) of short stature at 4 years in “first-borns having siblings”, “second-borns”, and “third-borns or more” were 1.08 (0.84–1.39), 1.36 (1.13–1.62), and 1.50 (1.20–1.88), respectively, versus “first-borns having no sibling”. Birth order was significantly associated with postnatal growth and may be a factor predisposing to short stature in early childhood.
Wind Characteristics and Temporal Trends in Eastern Paraná State, Brazil
Authors: de Bodas Terassi Paulo , Correia Filho Washington , Galvani Emerson , Oscar-Júnior Antonio , Sobral Bruno , de Gois Givanildo , Biffi Vitor , Oliveira Júnior José
Published: March 2023

The wind is one of the most important and studied variables globally, essential to several sectors, for example, energy. Therefore, this study assesses the wind regime and analysis trends in three locations within the Paraná state, Brazil. The historical series were recorded between 1976 and 2010 at conventional meteorological stations belonging to the Brazilian National Institute of Meteorology. WRPLOT version 8.0.0 software was used for elaborating wind roses and histograms in the annual and seasonal scales. Detection of trends and temporal rupture points was performed using different statistical methods (Run, Mann–Kendall, Pettitt and Shapiro–Wilk tests) for all meteorological stations. All statistical tests were conducted using the R software version 3.3.2. On a seasonal scale, summer and spring present the highest wind speeds in the Curitiba and Paranaguá stations due to meteorological systems on different scales, such as the South Atlantic subtropical anticyclone and frontal systems. The Mann–Kendall test revealed that Castro presented statistical significance in reducing wind speed, with a decrease of 0.23 m/s per decade for the annual scale and 0.23 m/s per decade during the autumn season. These ruptures indicated a decrease in wind speed in Curitiba and Paranaguá for the spring season. The Pettitt test revealed a break point detection in the data series in Curitiba station, likely due to urban expansion that started in the 1980s, reducing wind speed, especially in winter and spring. These trends and ruptures revealed a significant reduction in wind speed, possibly due to the interaction between natural climate changes and the increase in surface roughness resulting from land use and urbanization changes.
Effect of MoO3 Content on Ni3Al-Ag-MoO3 Composite Coating Microstructure and Tribological Properties
Authors: Fan Xiangjuan , Li Wensheng , Yang Jun , Zhu Shengyu , Cui Shuai , Cheng Bo , Zhai Haimin
Published: March 2023

In order to provide effective solid lubrication to Ni3Al coating, 10 wt.% Ag and different amounts of MoO3 solid lubricant were mechanically mixed with the SHSed Ni3Al powder and sprayed HVOF. Microstructure, mechanical properties, and tribological behavior from 25 °C to 800 °C of the coatings were studied, and the basic wear mechanisms were explored and discussed as well. Results show that the hardness and adhesive bonding strength of the coatings are slightly decreased, while there is little effect on the microstructure and mechanical properties of the Ni3Al-based composite coating when the content of MoO3 additive in the feedstock powder is lower than 15 wt.%. The composite coating formed by feedstock powder containing 15 wt.% MoO3 additive also presents excellent anti-friction and anti-wear performance from 25 °C to 800 °C, especially at 800 °C, where a complete, smooth, and thicker lubricating film comprised of NiO, Al2O3, MoO3, and Ag2MoO4 was formed, which reduced the friction coefficient (COF) and wear rate (WR) to the lowest value of 0.36 and 6.03 × 10−5 mm3/(Nm), respectively. An excessive amount of MoO3 in the feedstock powder (20 wt.%) results in inferior interlayer bonding of the formed coating, and the coating is more prone to delamination and abrasive wear above 200 °C.
Aging and the Impact of Solar Ultraviolet Radiation on the Expression of Type I and Type VI Collagen
Authors: Biskanaki Foteini , Kefala Vasiliki , Lazaris Andreas C. , Rallis Efstathios
Published: March 2023

Both endogenous and exogenous factors cause skin aging. This study aimed to compare the differential expressions of collagen type I (COL I) and collagen type VI (COL VI) in skins with biological aging and photoaging. In order to comprehend the impact of solar radiation in the dermis and the expression of COL I and COL VI, we studied the expression and their detection in healthy skin and in skin that had been characterized by aging. The hematoxylin and eosin staining protocol was performed in tissue paraffin blocks and they were then stained immunohistochemically with rabbit monoclonal anti-COL I and anti-COL VI antibodies. A total of 201 slides were studied with an Olympus BX 41 microscope, and the expressions of COL I and COL VI in the dermis were scored on a scale of 1 to 5, and then positively and statistically analyzed with IBM SPSS Statistics software. The results show that solar elastosis changes the structure of the skin’s collagen and solar elastosis was observed in the skin tissues with photoaging without appearing to be affected by its appearance in relation to age. Solar radiation divides the collagen fibers more rapidly than normal biological aging and replaces the collagen fibers of the skin. COL I and COL VI are expressed differently along the dermis of healthy skin tissue and the skin tissue subject to photoaging.
Anomalous Positron Lifetime in Single Crystal of Weyl Semimetal CoSi
Authors: Salamatin D. A. , Bokov A. V. , Kozin M. G. , Romashkina I. L. , Salamatin A. V. , Mikhin M. V. , Petrova A. E. , Sidorov V. A. , Nikolaev A. V. , Fisk Z. , Tsvyashchenko A. V.
Published: March 2023

The positron annihilation lifetimes were measured using a 48V positron source in noncentrosymmetric cubic single crystals of CoSi, FeSi and MnSi. The following lifetimes were determined from the positron annihilation time spectra: 168(1) ps for CoSi, 114(1) ps for FeSi and 111(1) ps for MnSi. For single-crystal CoSi, the positron annihilation lifetime was also determined with a 22Na positron source. For CoSi, the lifetimes obtained from different positron sources are consistent. The differences in the positron annihilation lifetimes in MnSi and FeSi, on the one hand, and in the Weyl semimetal CoSi, on the other hand, are possibly caused by the formation of a positron + electron bound state (positronium).
Catching the Green—Diversity of Ruderal Spring Plants Traditionally Consumed in Bulgaria and Their Potential Benefit for Human Health
Authors: Ivanova Teodora , Marchev Andrey , Chervenkov Mihail , Bosseva Yulia , Georgiev Milen , Kozuharova Ekaterina , Dimitrova Dessislava
Published: March 2023

The global climate and societal challenges in the recent years urge us to strengthen food security; thus, the rediscovery of wild foods and foraging practices is also part of the sustainability agenda. Utilization of underappreciated sources such as ruderal plants could be a valuable option, especially for vulnerable parts of the society. We present data on traditional knowledge on spring edible ruderal plant taxa preserved in rural regions of Bulgaria, combining field studies in the period 2017–2022 that were compared to the available recent and historical ethnographic and (ethno)botanical literature. Semi-structured interviews were performed with representatives of 94 households in North and South Bulgaria, focusing on collection practices, used parts, and preparation methods. We list 65 edible ruderals, belonging to 22 plant families, of which 19 appeared only in the literature sources. Unlike in the Mediterranean tradition, edible ruderal plants in Bulgaria were regarded unfavorably, as poverty food. Amaranthaceae and Asteraceae were the most represented families, with 10 taxa each. About half of the taxa were collected for their leaves or whole young herbage that is used as pastry fillings, in stewed, and in cooked dishes. Taxa used in raw salads were mostly from the literature sources. The most diverse utilization was recorded in the southern-most regions of Bulgaria, where immediate tasting of the gathered plants was reported by the participants as the way to collect food plants. The bitter ones or those with an unappealing smell were considered non-edible and were avoided. References about biologically active compounds and potential benefits were collected, classified, and discussed in regard to their potential benefits for human health.
Performance Evaluation of a BZ COVID-19 NALF Assay for Rapid Diagnosis of SARS-CoV-2
Authors: Jang Woong Sik , Jee Hyunseul , Lee Joon Min , Lim Chae Seung , Kim Jeeyong
Published: March 2023

Coronavirus disease (COVID-19) caused by SARS-CoV-2 infection has been a global pandemic for more than two years, and it is important to quickly and accurately diagnose and isolate patients with SARS-CoV-2 infection. The BZ COVID-19 NALF Assay could sensitively detect SARS-CoV-2 from a nasopharyngeal swab because it adopts both a loop-mediated isothermal amplification and lateral flow immunochromatography technology. In this study, a total of 389 nasopharyngeal swab samples, of which 182 were SARS-CoV-2 PCR positive and 207 were negative samples, were recruited. Compared to the Allplex™ SARS-CoV-2 Assay, the BZ COVID-19 NALF Assay showed 95.05% sensitivity and 99.03% specificity for detecting SARS-CoV-2. The concordance rate between the BZ COVID-19 NALF Assay and Allplex™ SARS-CoV-2 Assay was 97.69%. The turnaround time of the BZ COVID-19 NALF Assay is only about 40~55 min. The BZ COVID-19 NALF Assay is an accurate, easy, and quick molecular diagnostic test compared to the conventional PCR test for detection of SARS-CoV-2. In addition, the BZ COVID-19 NALF Assay is thought to be very useful in small size medical facilities or developing countries where it is difficult to operate a clinical laboratory.
KCS-FCnet: Kernel Cross-Spectral Functional Connectivity Network for EEG-Based Motor Imagery Classification
Authors: García-Murillo Daniel Guillermo , Álvarez-Meza Andrés Marino , Castellanos-Dominguez Cesar German
Published: March 2023

This paper uses EEG data to introduce an approach for classifying right and left-hand classes in Motor Imagery (MI) tasks. The Kernel Cross-Spectral Functional Connectivity Network (KCS-FCnet) method addresses these limitations by providing richer spatial-temporal-spectral feature maps, a simpler architecture, and a more interpretable approach for EEG-driven MI discrimination. In particular, KCS-FCnet uses a single 1D-convolutional-based neural network to extract temporal-frequency features from raw EEG data and a cross-spectral Gaussian kernel connectivity layer to model channel functional relationships. As a result, the functional connectivity feature map reduces the number of parameters, improving interpretability by extracting meaningful patterns related to MI tasks. These patterns can be adapted to the subject’s unique characteristics. The validation results prove that introducing KCS-FCnet shallow architecture is a promising approach for EEG-based MI classification with the potential for real-world use in brain–computer interface systems.
Distributed Multi-Robot-Trailer Scheduling Based on Communication between Charging Stations for Robot Being Towed to Recharge
Authors: Deng Yanfang , Li Taijun , Xie Mingshan , Zhang Song
Published: March 2023

It is necessary for robot-trailer to tow robot to the charging station for recharging when the robot fails, or the battery cannot support the robot to the charging station. However, the delay of towing robot is affecting the working efficiency of mobile robot. Based on the task priority of the mobile robot and impact degree on the room after the power failure, the paper proposes a distributed scheduling of robot being towed to recharge for reducing the delay expectation. This work designs a Distributed Three Nodes Service (DTNS) scheduling based on the communication between charging stations. The two-step path-planner based on decision factor and travel path is used in the scheme. Simulations show that the distributed scheduling of this work can well ensure the success communication in the case of low power, and DTNS can well reduce the delay expectation of towing robot to recharge. Compared with First Come First Service (FCFS) scheduling, DTNS reduces the towing delay by 48.71%, 48.83% and 40.45% when there are some robots sending the towing request, and by 58.77%, 39.97% and 41.90% when no robot sends request in the case of 1, 2 and 3 robot trailers in the service space respectively.
IoT Network Model with Multimodal Node Distribution and Data-Collecting Mechanism Using Mobile Clustering Nodes
Authors: Vorobyova Darya , Muthanna Ammar , Paramonov Alexander , Markelov Oleg A. , Koucheryavy Andrey , Ali Gauhar , ElAffendi Mohammed , Abd El-Latif Ahmed A.
Published: March 2023

In this paper, the novel study of an Internet of Things (IoT) network model with multimodal node distribution and a data-collecting mechanism using mobile clustering nodes is presented. The aim of this work is to introduce the problem of organizing the mobile cluster head IoT network with a heterogeneous distribution node in the service area with multimodal distribution nodes. A new method for clustering a heterogeneous network is proposed, which makes it possible to efficiently identify clusters that differ in terms of the density of nodes. This makes it possible to choose the speed of the mobile cluster head in accordance with the density in each cluster. The proposed method uses the Density-Based Spatial Clustering of Applications with Noise (DBSCAN) clustering algorithm. One of the benefits of our proposed model is the increase in the efficiency of using a mobile cluster head. The new solution can be used to organize data collection in the IoT.
The Use of Evolutionary Algorithms in the Modelling of Diffuse Radiation in Terms of Simulating the Energy Efficiency of Photovoltaic Systems
Authors: Olchowik Wiktor , Gajek Jędrzej , Michalski Andrzej
Published: March 2023

In light of the rapidly growing number of photovoltaic micro-grids, the modelling of their short-term power yields based on meteorological measurements is increasing in significance. This requires the knowledge of total and diffuse instantaneous solar radiation; however, most meteorological stations conduct actinometric measurements only with regard to total solar radiation, especially on a minute scale. This paper contains an analysis of the currently used PV cell mathematical model and suggests its modification aimed at calculating PV cell power with satisfactory accuracy, without the knowledge of diffuse solar radiation. Three function families were proposed to approximate the relationship between diffuse irradiance and the total and theoretical total irradiance variance for a cloudless sky. A program has been implemented to identify functions from the aforementioned function families. It leverages an evolution strategy algorithm and a fitness function based on the least-squares point method. It was employed to calculate the desired functions based on actual measurement data. The outcome was the sought-after dependence that enables predicting diffuse irradiance based on more frequently available measurement data.
Influence of the Reward Function on the Selection of Reinforcement Learning Agents for Hybrid Electric Vehicles Real-Time Control
Authors: Acquarone Matteo , Maino Claudio , Misul Daniela , Spessa Ezio , Mastropietro Antonio , Sorrentino Luca , Busto Enrico
Published: March 2023

The real-time control optimization of electrified vehicles is one of the most demanding tasks to be faced in the innovation progress of low-emissions mobility. Intelligent energy management systems represent interesting solutions to solve complex control problems, such as the maximization of the fuel economy of hybrid electric vehicles. In the recent years, reinforcement-learning-based controllers have been shown to outperform well-established real-time strategies for specific applications. Nevertheless, the effects produced by variation in the reward function have not been thoroughly analyzed and the potential of the adoption of a given RL agent under different testing conditions is still to be assessed. In the present paper, the performance of different agents, i.e., Q-learning, deep Q-Network and double deep Q-Network, are investigated considering a full hybrid electric vehicle throughout multiple driving missions and introducing two distinct reward functions. The first function aims at guaranteeing a charge-sustaining policy whilst reducing the fuel consumption (FC) as much as possible; the second function in turn aims at minimizing the fuel consumption whilst ensuring an acceptable battery state of charge (SOC) by the end of the mission. The novelty brought by the results of this paper lies in the demonstration of a non-trivial incapability of DQN and DDQN to outperform traditional Q-learning when a SOC-oriented reward is considered. On the contrary, optimal fuel consumption reductions are attained by DQN and DDQN when more complex FC-oriented minimization is deployed. Such an important outcome is particularly evident when the RL agents are trained on regulatory driving cycles and tested on unknown real-world driving missions.