bluuebunny/crossref_metadata_2025 huggingface dataset:
Process-Induced Changes in the Quantity and Characteristics of Grain Dietary Fiber
Authors: Maina Ndegwa H. , Rieder Anne , De Bondt Yamina , Mäkelä-Salmi Noora , Sahlstrøm Stefan , Mattila Outi , Lamothe Lisa M. , Nyström Laura , Courtin Christophe M. , Katina Kati , Poutanen Kaisa
Published: October 2021

Daily use of wholegrain foods is generally recommended due to strong epidemiological evidence of reduced risk of chronic diseases. Cereal grains, especially the bran part, have a high content of dietary fiber (DF). Cereal DF is an umbrella concept of heterogeneous polysaccharides of variable chemical composition and molecular weight, which are combined in a complex network in cereal cell walls. Cereal DF and its distinct components influence food digestion throughout the gastrointestinal tract and influence nutrient absorption and other physiological reactions. After repeated consumption of especially whole grain cereal foods, these effects manifest in well-demonstrated health benefits. As cereal DF is always consumed in the form of processed cereal food, it is important to know the effects of processing on DF to understand, safeguard and maximize these health effects. Endogenous and microbial enzymes, heat and mechanical energy during germination, fermentation, baking and extrusion destructurize the food and DF matrix and affect the quantity and properties of grain DF components: arabinoxylans (AX), beta-glucans, fructans and resistant starch (RS). Depolymerization is the most common change, leading to solubilization and loss of viscosity of DF polymers, which influences postprandial responses to food. Extensive hydrolysis may also remove oligosaccharides and change the colonic fermentability of DF. On the other hand, aggregation may also occur, leading to an increased amount of insoluble DF and the formation of RS. To understand the structure–function relationship of DF and to develop foods with targeted physiological benefits, it is important to invest in thorough characterization of DF present in processed cereal foods. Such understanding also demands collaborative work between food and nutritional sciences.
Elaboration of a Phytomedicine for Intestinal Comfort Based on the European Union Regulation on Traditional Use: Mixture Design Optimization
Authors: Es-safi Imane , Mechchate Hamza , Amaghnouje Amal , Jawhari Fatima Zahra , Bousta Dalila
Published: October 2021

Nowadays, knowledge about the uses of medicinal plants is growing exponentially, and in order to conserve and valorize this knowledge, the European Union has set up multiple regulations to control their usage by the population. In this framework, this work is based on EU regulations to elaborate a new phytomedicine for intestinal comfort. The phytomedicine subject of elaboration is under the category of Traditional Use Herbal Medicine Products (TUHMPs), containing plants of approved medicinal use of at least 30 years, including 15 years in the European Union (Myrtus communis L. leaves (MC), Pimpinella anisum L. seeds (PA) and Carum carvi L. (CC) fruit). The EU pharmacopeia and monographs recognize the individual effect of each plant selected, and this work aims to study their combined effect using a mixture design. Acetic acid-induced writhing test in mice is used to study the efficacy of the mixture as pain relief and for intestinal comfort. According to the test result, the individual, binary and ternary combination were very effective compared to negative and positive control approving by that the synergy between the plants. Following the mixture design analysis, the best formulation was a ternary combination with different ratios (MC: 45%, PA: 52% and CC with 3%). The predicted formulation was studied after that and approved by the test result. This work provided a practical way to establish new phytomedicines within the EU and could be a model for new outcoming phytomedicine in the market.
miR-301a-5p Regulates TGFB2 during Chicken Spermatogenesis
Authors: Guo Qixin , Jiang Yong , Bai Hao , Chen Guohong , Chang Guobin
Published: October 2021

The process of spermatogenesis is complex and systemic, requiring the cooperation of many regulators. However, little is known about how micro RNAs (miRNAs) regulate spermatogenesis in poultry. In this study, we investigated key miRNAs and their target genes that are involved in spermatogenesis in chickens. Next-generation sequencing was conducted to determine miRNA expression profiles in five cell types: primordial germ cells (PGCs), spermatogonial stem cells (SSCs), spermatogonia (Spa), and chicken sperm. Next, we analyzed and identified several key miRNAs that regulate spermatogenesis in the four germline cell miRNA profiles. Among the enriched miRNAs, miRNA-301a-5p was the key miRNA in PGCs, SSCs, and Spa. Through reverse transcription quantitative PCR (RT-qPCR), dual-luciferase, and miRNA salience, we confirmed that miR-301a-5p binds to transforming growth factor-beta 2 (TGFβ2) and is involved in the transforming growth factor-beta (TGF-β) signaling pathway and germ cell development. To the best of our knowledge, this is the first demonstration of miR-301a-5p involvement in spermatogenesis by direct binding to TGFβ2, a key gene in the TGF-β signaling pathway. This finding contributes to the insights into the molecular mechanism through which miRNAs regulate germline cell differentiation and spermatogenesis in chickens.
Effects of Patient-Selected Music Listening on the Pain and Anxiety of Patients Undergoing Hemodialysis: A Randomized Controlled Trial
Authors: Kim SukKyong , Jeong HyeonCheol
Published: October 2021

This study aimed to analyze the influence of patient-selected music listening on the pain and anxiety levels of hemodialysis patients after undergoing a vascular access operation. Methods: Sixty five patients were randomly assigned to the experimental group (n = 32) or the control group (n = 33). The experimental group was instructed to listen to their favorite music using headphones during their operations at the center. The control group underwent the operations without listening to any music. The pain measurement during vascular access operation was measured by subjective pain and objective pain behavior; anxiety was measured by subjective anxiety and anxiety states. Results: The experimental group reported significantly lower subjective pain levels than the control group (t = 9.36, p = 0.003). Regarding objective pain behaviors, the experimental group had a significantly lower score than the control group (t = 4.59, p = 0.036). The experimental group had significantly lowered subjective anxiety compared to the control group (F = 10.10, p = 0.002). Regarding anxiety states, the experimental group had significantly lower scores than the control group (F = 23.34, p < 0.001). Conclusion: The results suggest that patient-selected music listening reduced hemodialysis patients’ pain and anxiety levels during vascular access operations. Therefore, music medicine can be included as a new clinical intervention.
The Impact of Psychological Interventions with Elements of Mindfulness (PIM) on Empathy, Well-Being, and Reduction of Burnout in Physicians: A Systematic Review
Authors: Tement Sara , Ketiš Zalika Klemenc , Miroševič Špela , Selič-Zupančič Polona
Published: October 2021

Introduction: Physician’s burnout has been recognized as an increasing and significant work-related syndrome, described by the combination of emotional exhaustion (EE) and depersonalization (D), together with low personal accomplishment (PA). It has many negative consequences on personal, organizational, and patient care levels. This systematic review aimed to analyze research articles where psychological interventions with elements of mindfulness (PIMs) were used to support physicians in order to reduce burnout and foster empathy and well-being. Methods: Systematic searches were conducted in May 2019, within six electronic databases PubMed, EBSCOhost MEDLINE, PsycArticles, Cochrane Library, JSTOR, and Slovenian national library information system. Different combinations of boolean operators were used—mindfulness, empathy, medicine/family medicine/general practice/primary care, burnout, doctors/physicians, intervention, and support group. Additional articles were manually searched from the reference list of the included articles. Studies with other healthcare professionals (not physicians and residents) and/or medical students, and those where PIMs were applied for educational or patient’s treatment purposes were excluded. Results: Of 1194 studies identified, 786 screened and 139 assessed for eligibility, there were 18 studies included in this review. Regardless of a specific type of PIMs applied, results, in general, demonstrate a positive impact on empathy, well-being, and reduction in burnout in participating physicians. Compared with other recent systematic reviews, this is unique due to a broader selection of psychological interventions and emphasis on a sustained effect measurement. Conclusions: Given the pandemic of COVID-19, it is of utmost importance that this review includes also interventions based on modern information technologies (mobile apps) and can be used as an awareness-raising material for physicians providing information about feasible and easily accessible interventions for effective burnout prevention and/or reduction. Future research should upgrade self-reported data with objective psychological measures and address the question of which intervention offers more benefits to physicians.
Adaptation to Disaster Risk—An Overview
Authors: Jia Huicong , Chen Fang , Du Enyu
Published: October 2021

The role of natural disaster adaptation is increasingly being considered in academic research. The Paris Agreement and Sustainable Development Goal 13 require measuring the progress made on this adaptation. This review summarizes the development stages of adaptation, the multiple attributes and analysis of adaptation definitions, the models and methods for adaptation analysis, and the research progress of natural disaster adaptation. Adaptation research methods are generally classified into two types: case analysis and mathematical models. The current adaptive research in the field of natural disasters focuses primarily on the response of the social economy, especially the adaptive decision making and risk perception at farm-level scales (farmer households). The evaluation cases of adaptation in the field of disasters exist mostly as a part of vulnerability evaluation. Adaptation and adaptive capacity should focus on four core issues: adaptation to what; who or what adapts; how does adaptation occur; what is adaptation; and how good is the adaptation. The main purpose of the “spatial scale–exposure–vulnerability” three-dimensional scales of adaptation assessment is to explore the differences in index system under different scenarios, the spatial pattern of adaptations, and the geographical explanation of its formation mechanism. The results of this study can help and guide future research on integrating climate change and disaster adaptations especially in regional sustainable development and risk reduction strategies.
Mechanism by Which MC Controls Harmful Algal Blooms Revealed by Cell Morphology of Aureococcus anophagefferens
Authors: Zhu Jianan , Yu Zhiming , He Liyan , Cao Xihua , Ji Hena , Song Xiuxian
Published: October 2021

On the basis of field experience, a bloom does not continue after treatment with modified clay (MC), even though the residual harmful algal bloom (HAB) biomass accounts for 20–30% of the initial cells. This interesting phenomenon indicates that, in addition to causing flocculation, MC can inhibit the growth of residual cells. Here, from a cell morphology perspective, Aureococcus anophagefferens was used as a model organism to explore this scientific issue and clarify the mechanism by which MC mitigates harmful algal blooms (HABs). The results showed that, at an ~70% removal efficiency, neutral clay (NC) could not effectively inhibit the growth of residual cells, although it caused various forms of damage to residual cells, such as cell deformation, cell breakage, decreased extracellular polysaccharides (EPS), increased cell membrane permeability, and increased cytoplasmic granularity, due to physical collisions. After modification, some physical and chemical properties of the clay particle surface were changed; for example, the surface electrical properties changed from negative to positive, lamellar spacing increased, hardness decreased, adhesion chains increased, adhesion improved, and the number of absorption sites increased, enhancing the occurrence of chemical and electrochemical effects and physical collisions with residual cells, leading to severe cell deformation and chemical cell breakage. Thus, MC effectively inhibited the growth of residual cells and controlled HABs.
Gendering Neighbourhood Marginalization Metrics in Mental Health Services Research: A Cross-Sectional Exploration of a Rural and Small Urban Population
Authors: Gupta Neeru , Crouse Dan Lawson , Foroughi Ismael , Nikolaidou Thalia
Published: October 2021

Background: Little is known about the extent to which socioenvironmental characteristics may influence mental health outcomes in smaller population centres or differently among women and men. This study used a gender-based analysis approach to explore individual- and neighbourhood-level sex differences in mental health service use in a context of uniquely smaller urban and rural settlements. Methods: This cross-sectional analysis leveraged multiple person-based administrative health datasets linked with geospatial datasets among the population aged 1 and over in the province of New Brunswick, Canada. We used multinomial logistic regression to examine associations between neighbourhood characteristics with risk of service contacts for mood and anxiety disorders in 2015/2016, characterizing the areal measures among all residents (gender neutral) and by males and females separately (gender specific), and controlling for age group. Results: Among the province’s 707,575 eligible residents, 10.7% (females: 14.0%; males: 7.3%) used mental health services in the year of observation. In models adjusted for gender-neutral neighbourhood characteristics, service contacts were significantly more likely among persons residing in the most materially deprived areas compared with the least (OR = 1.09 [95% CI: 1.05–1.12]); when stratified by individuals’ sex, the risk pattern held for females (OR = 1.13 [95% CI: 1.09–1.17]) but not males (OR = 1.00 [95% CI: 0.96–1.05]). Residence in the most female-specific materially deprived neighbourhoods was independently associated with higher risk of mental health service use among individual females (OR = 1.08 [95% CI: 1.02–1.14]) but not among males (OR = 1.02 [95% CI: 0.95–1.10]). Conclusion: These findings emphasize that research needs to better integrate sex and gender in contextual measures aiming to inform community interventions and neighbourhood designs, notably in small urban and rural settings, to reduce socioeconomic inequalities in the burden of mental disorders.
Normalization of Non-Drinking? Health, School Situation and Social Relations among Swedish Ninth Graders That Drink and Do Not Drink Alcohol
Authors: Raninen Jonas , Larm Peter , Svensson Johan , Livingston Michael , Sjödin Lars , Karlsson Patrik
Published: October 2021

Alcohol consumption is a major contributor to the disease burden among adolescents. The adolescent alcohol abstainer is still often depicted as problematic in the research literature and in prominent theoretical frameworks. However, over the past two decades, there has been a marked trend of declining youth drinking in Sweden. The declining trend has led to a shift in the majority behaviour of youth, from drinking to non-drinking. It is plausible that this trend has also shifted the position of non-drinkers. This paper examines the position of non-drinkers in a nationally representative sample of Swedish adolescents. A survey was carried out in 2017 in 500 randomly selected schools. A total of 5549 respondents (15–16-year-olds) agreed to participate and answered the questionnaire. A minority (42.8%) had consumed alcohol during their lifetime. The results show that non-drinkers had better health and school performance when compared to drinkers. The results also showed that there were no differences in the social position between non-drinkers and drinkers. These findings are new and indicate a changed position of non-drinkers among Swedish adolescents. With non-drinking being the majority behaviour among Swedish adolescents this seems to have shifted the position of non-drinkers. There is a need for research on the long-term importance of not drinking during adolescence.
Alcohol Drinking Pattern and Risk of Head and Neck Cancer: A Nationwide Cohort Study
Authors: Koo Hye Yeon , Han Kyungdo , Shin Dong Wook , Yoo Jung Eun , Cho Mi Hee , Jeon Keun Hye , Kim Dahye , Hong Sangduk , Jun Jae Kwan
Published: October 2021

Alcohol consumption is a major risk factor for head and neck cancer (HNC), yet little data exist examining drinking patterns and HNC risk. In this population-based, retrospective cohort study, 11,737,467 subjects were recruited from the Korean National Health Insurance Service database. The risks of overall HNC and HNC subtypes according to average alcohol consumption, drinking frequency, and daily amount were examined using Cox proportional hazard models. Over the median follow-up of 6.4 years, 15,832 HNC cases were identified. HNC risk linearly increased with drinking frequency (p-trend < 0.01; adjusted hazard ratio [aHR] 1.55, 95% confidence interval [CI] 1.45–1.67 in subjects who drank 7 days/week). HNC risk also increased according to daily amount of alcohol consumption (p-trend < 0.01), but plateaued from 5–7 units/occasion (aHR 1.25, 95% CI 1.19–1.31) to >14 units/occasion (aHR 1.26, 95% CI 1.13–1.40). When stratified by average alcohol consumption, drinking frequency, but not daily amount, showed a linear relationship with HNC risk in moderate and heavy drinkers. When comparing the HNC subtypes, similar tendencies were observed in cancers of the oral cavity, pharynx, and larynx, but not in the salivary gland. In conclusion, drinking frequency is a stronger risk factor for HNC, especially for cancer of the oral cavity, pharynx, and larynx, than the daily amount of alcohol consumption.
COVID-19 Impact on Residential Preferences in the Early-Stage Outbreak in South Korea
Authors: Kang Bumjoon , Won Jaewoong , Kim Eun Jung
Published: October 2021

In the early stage of the COVID-19 pandemic in South Korea, public fear or social scaring of urban living was observed, which caused people to change their daily routines. This study examines how the COVID-19 pandemic affected residential choice and perceptions of urban living. We analyzed self-reported survey data collected from 2000 participants in Seoul, Daegu, and Kyeongbuk in South Korea between 3–6 August 2020, targeting the relatively controlled period after the first COVID-19 outbreak. Logistic regression models were used to examine concerns of urban living and residence relocation consideration. Those who were aged 30 or older, regularly commuting, not feeling healthy, with a household size of two, and living in a low-rise condominium were more likely to be concerned with urban living. Those who were aged 40 or older and living in a townhouse or a single-detached house were more likely to consider moving to a less dense area. People perceived that their daily routine changed substantially after the pandemic. Certain participant groups showed concerns of urban living and relocation consideration, suggesting housing policy implications.
Role of Libraries in Human Flourishing: Adolescents’ Motivational Orientation for Occupation
Authors: Matsuyama Yusuke , Fujiwara Takeo
Published: October 2021

Introduction: Adolescence is crucial for human flourishing and strongly influences having meaning in life. We investigated the association between local public library density as a shared resource and motivational orientation toward their occupation in Japanese adolescents. Methods: A longitudinal study was conducted using data from a nationwide birth cohort survey in Japan (n = 12,184). At age 7, their caregivers answered questionnaires on children including the number of books read. Library density (low, moderate, or high) in each municipality was obtained from national statistics. At age 15, the adolescents indicated whether they had decided on an occupation and selected motivational orientations from among intrinsic (own ability and interest), extrinsic (high earnings, social class, or job stability), and altruistic (social contribution) orientations. Multilevel linear probability models were fitted, adjusting for confounders, including household socioeconomic status and city size. Results: Intrinsic, extrinsic, and altruistic motivations for desired occupation were reported by 40.7%, 31.9% and 41.8% of participants, respectively. Living in a municipality with a high library density at age 7 was associated with having intrinsic motivation at age 15 than low density by 3.1 percentage points (95% confidence interval (CI): 0.35, 5.85). The association was more prominent for those with lower income (P for interaction = 0.026). Neither extrinsic nor altruistic motivations were associated with library density (coefficient: −0.13; 95% CI: −2.81, 2.56; coefficient: 0.08; 95% CI: −2.72, 2.88 percentage points, respectively). Conclusions: Developing libraries in communities could encourage intrinsic motivation in adolescents, specifically for those in low-income households.
Crime Prevention Based on the Strategic Mapping of Living Conditions
Authors: Guldåker Nicklas , Hallin Per-Olof , Nilvall Kim , Gerell Manne
Published: October 2021

This paper presents a theoretically and methodologically grounded GIS-based model for the measurement and mapping of an index of living conditions in urban residential areas across Sweden. Further, the model is compared and evaluated using the Swedish Police’s assessment of crime-exposed areas. The results indicate that the geographically measured vulnerable living conditions overlap to a large extent with the areas assessed to be crime-exposed by the Swedish Police. Over 61% of the police-defined crime-exposed areas are characterized by vulnerable living conditions. The results also show that this overlap is not perfect and that there are vulnerable areas that are not included in the police’s assessment of crime-exposed areas, but which are nonetheless characterized by vulnerable living conditions that could negatively affect the development of crime. It is also proposed that the model and the mapped index of living conditions can provide a more well-grounded scientific basis for the police’s assessment work. As a first step, the Swedish police have implemented the model and the mapped index in the work process employed in their annual identification of crime-exposed or at-risk areas. In addition to assisting the police, the model and the mapped index could also be used to support other societal actors working with vulnerable areas.
Revising the Classic Computing Paradigm and Its Technological Implementations
Authors: Végh János
Published: October 2021

Today’s computing is based on the classic paradigm proposed by John von Neumann, three-quarters of a century ago. That paradigm, however, was justified for (the timing relations of) vacuum tubes only. The technological development invalidated the classic paradigm (but not the model!). It led to catastrophic performance losses in computing systems, from the operating gate level to large networks, including the neuromorphic ones. The model is perfect, but the paradigm is applied outside of its range of validity. The classic paradigm is completed here by providing the “procedure” missing from the “First Draft” that enables computing science to work with cases where the transfer time is not negligible apart from the processing time. The paper reviews whether we can describe the implemented computing processes by using the accurate interpretation of the computing model, and whether we can explain the issues experienced in different fields of today’s computing by omitting the wrong omissions. Furthermore, it discusses some of the consequences of improper technological implementations, from shared media to parallelized operation, suggesting ideas on how computing performance could be improved to meet the growing societal demands.
Towards Standardisation of Technique for En Bloc Sacrectomy for Locally Advanced and Recurrent Rectal Cancer
Authors: Rogers Ailín C. , Jenkins John T. , Rasheed Shahnawaz , Malietzis George , Burns Elaine M. , Kontovounisios Christos , Tekkis Paris P.
Published: October 2021

Treatment strategies for advanced or recurrent rectal cancer have evolved such that the ultimate surgical goal to achieve a cure is complete pathological clearance. To achieve this where the sacrum is involved, en bloc sacrectomy is the current standard of care. Sacral resection is technically challenging and has been described; however, the technique has yet to be streamlined across units. This comprehensive review aims to outline the surgical approach to en bloc sacrectomy for locally advanced or recurrent rectal cancer, with standardisation of the operative steps of the procedure and to discuss options that enhance the technique.
Long-Term Weight Outcomes after Bariatric Surgery: A Single Center Saudi Arabian Cohort Experience
Authors: Alfadda Assim A. , Al-Naami Mohammed Y. , Masood Afshan , Elawad Ruba , Isnani Arthur , Ahamed Shaik Shaffi , Alfadda Nora A.
Published: October 2021

Background: Obesity is considered a global chronic disease requiring weight management through lifestyle modification, pharmacotherapy, or weight loss surgery. The dramatic increase in patients with severe obesity in Saudi Arabia is paralleled with those undergoing bariatric surgery. Although known to be beneficial in the short term, the long-term impacts of surgery within this group and the sustainability of weight loss after surgery remains unclear. Objectives: We aimed to assess the long-term weight outcomes after bariatric surgery. Setting: The study was conducted at King Khalid University Hospital (KKUH), King Saud University Medical City (KSUMC) in Riyadh, Saudi Arabia. Methods: An observational prospective cohort study on adult patients with severe obesity undergoing bariatric surgery (sleeve gastrectomy (SG) or Roux-en Y gastric bypass (RYGB)) during the period between 2009 and 2015 was conducted. Weight loss patterns were evaluated pre- and post-surgery through clinical and anthropometric assessments. Absolute weight loss was determined, and outcome variables: percent excess weight loss (%EWL), percent total weight loss (%TWL), and percent weight regain (%WR), were calculated. Statistical analysis using univariate and multivariate general linear modelling was carried out. Results: A total of 91 (46 males and 45 females) patients were included in the study, with the majority belonging to the SG group. Significant weight reductions were observed at 1 and 3 years of follow-up (p < 0.001) from baseline. The %EWL and %TWL were at their maximum at 3 years (72.4% and 75.8%) and were comparable between the SG and RYGB. Decrements in %EWL and %TWL and increases in %WR were seen from 3 years onwards from bariatric surgery until the study period ended. The yearly follow-up attrition rate was 20.8% at 1 year post-surgery, 26.4% at year 2, 31.8% at year 3, 47.3% at year 4, 62.6% at year 5, and 79.1% at end of study period (at year 6). Conclusion: The major challenge to the successful outcome of bariatric surgery is in maintaining weight loss in the long-term and minimizing weight regain. Factors such as the type of surgery and gender need to be considered before and after surgery, with an emphasis on the need for long-term follow-up to enssure the optimal benefits from this intervention.
The Influence of Lymph Node Count on Oncological Outcome of Radical Cystectomy in Chemotherapy Pre-Treated and Chemotherapy-Naïve Patients with Muscle Invasive Bladder Cancer
Authors: Lemiński Artur , Kaczmarek Krystian , Michalski Wojciech , Małkiewicz Bartosz , Kotfis Katarzyna , Słojewski Marcin
Published: October 2021

Radical cystectomy (RC) with pelvic lymph node dissection (PLND) remains the mainstay of treatment for muscle-invasive bladder cancer (MIBC). The extent of PLND and number of removed lymph nodes (LNs) have been associated with improved staging and survival outcomes in several series of RC patients. Neoadjuvant chemotherapy (NAC) has become standard of care for cisplatin-eligible patients qualified to RC, yet few studies on PLND stratified cases according to the receipt of NAC. We aimed to address this issue and reevaluate the prognostic value of PLND nodal yields in series of patients who underwent RC on the verge of the NAC era. This single-center, retrospective, clinical follow-up study enrolled 439 consecutive patients, out of whom 83 received NAC. We analyzed survival outcome of RC according to the number of removed nodes between NAC and non-NAC subgroups. We found PLND thresholds of 10 and 15 LNs prognostically meaningful in our study cohort, and this association was particularly pronounced in the non-NAC subgroup. Higher numbers of LNs provided a 25% reduction in risk of all-cause mortality and correspondingly correlated with up to a 14% increase in 3-year overall survival. The receipt of NAC diminished the benefit of adequate PLND, as the number of retrieved LNs was not associated with survival in the NAC-RC cohort. Given the limitations of our study, additional research is needed to verify these findings.
Allografts for Skin Closure during In Utero Spina Bifida Repair in a Sheep Model
Authors: Mann Lovepreet K. , Won Jong Hak , Patel Rajan , Bergh Eric P. , Garnett Jeannine , Bhattacharjee Meenakshi B. , Narayana Ponnada A. , Jain Ranu , Fletcher Stephen A. , Lai Dejian , Papanna Ramesha
Published: October 2021

Objectives: Use of off-label tissue graft materials, such as acellular dermal matrix (ADM), for in utero repair of severe spina bifida (SB), where primary skin layer closure is not possible, is associated with poor neurological outcomes. The cryopreserved human umbilical cord (HUC) patch has regenerative, anti-inflammatory, and anti-scarring properties, and provides watertight SB repair. We tested the hypothesis that the HUC is a superior skin patch to ADM for reducing inflammation at the repair site and preserving spinal cord function. Methods: In timed-pregnant ewes with twins, on gestational day (GD) 75, spina bifida was created without a myelotomy (functional model). On GD 95, repair was performed using HUC vs. ADM patches (randomly assigned) by suturing them to the skin edges. Additionally, full thickness skin closure as a primary skin closure (PSC) served as a positive control. Delivery was performed on GD 140, followed by blinded to treatment neurological assessments of the lambs using the Texas Spinal Cord Injury Scale (TSCIS) for gait, proprioception, and nociception. Lambs without spina bifida were used as controls (CTL). Ex vivo magnetic resonance imaging of spines at the repair site were performed, followed by quantitative pathological assessments. Histological assessments (blinded) included Masson’s trichrome, and immunofluorescence for myeloperoxidase (MPO; neutrophils) and for reactive astrocytes (inflammation) by co-staining vimentin and GFAP. Results: The combined hind limbs’ TSCIS was significantly higher in the HUC group than in ADM and PSC groups, p = 0.007. Both ADM and PSC groups exhibited loss of proprioception and mild to moderate ataxia compared to controls. MRI showed increased pathological findings in the PSC group when compared to the HUC group, p = 0.045. Histologically, the meningeal layer was thickened (inflammation) by 2–3 fold in ADM and PSC groups when compared to HUC and CTL groups, p = 0.01. There was lower MPO positive cells in the HUC group than in the ADM group, p = 0.018. Posterior column astrocyte activation was increased in ADM and PSC lambs compared to HUC lambs, p = 0.03. Conclusion: The HUC as a skin patch for in utero spina bifida repair preserves spinal cord function by reducing underlying inflammation when compared to ADM.
Evaluation of Age and Sex-Related Metabolic Changes in Healthy Subjects: An Italian Brain 18F-FDG PET Study
Authors: Allocca Michela , Linguanti Flavia , Calcagni Maria Lucia , Cistaro Angelina , Gaudieri Valeria , Guerra Ugo Paolo , Morbelli Silvia , Nobili Flavio , Pappatà Sabina , Sestini Stelvio , Volterrani Duccio , Berti Valentina ,   for the Neurology Study Group of the Italian Association of Nuclear Medicine
Published: October 2021

Background: 18F-fluorodeoxyglucose (18F-FDG) positron-emission-tomography (PET) allows detection of cerebral metabolic alterations in neurological diseases vs. normal aging. We assess age- and sex-related brain metabolic changes in healthy subjects, exploring impact of activity normalization methods. Methods: brain scans of Italian Association of Nuclear Medicine normative database (151 subjects, 67 Males, 84 Females, aged 20–84) were selected. Global mean, white matter, and pons activity were explored as normalization reference. We performed voxel-based and ROI analyses using SPM12 and IBM-SPSS software. Results: SPM proved a negative correlation between age and brain glucose metabolism involving frontal lobes, anterior-cingulate and insular cortices bilaterally. Narrower clusters were detected in lateral parietal lobes, precuneus, temporal pole and medial areas bilaterally. Normalizing on pons activity, we found a more significant negative correlation and no positive one. ROIs analysis confirmed SPM results. Moreover, a significant age × sex interaction effect was revealed, with worse metabolic reduction in posterior-cingulate cortices in females than males, especially in post-menopausal age. Conclusions: this study demonstrated an age-related metabolic reduction in frontal lobes and in some parieto-temporal areas more evident in females. Results suggested pons as the most appropriate normalization reference. Knowledge of age- and sex-related cerebral metabolic changes is critical to correctly interpreting brain 18F-FDG PET imaging.
Sarcopenia, Obesity, and Sarcopenic Obesity: Relationship with Skeletal Muscle Phenotypes and Single Nucleotide Polymorphisms
Authors: Khanal Praval , Williams Alun G. , He Lingxiao , Stebbings Georgina K. , Onambele-Pearson Gladys L. , Thomis Martine , Degens Hans , Morse Christopher I.
Published: October 2021

Obesity may aggravate the effects of sarcopenia on skeletal muscle structure and function in the elderly, but no study has attempted to identify the gene variants associated with sarcopenia in obese women. Therefore, the aims of the present study were to: (1) describe neuromuscular function in sarcopenic and non-sarcopenic women with or without obesity; (2) identify gene variants associated with sarcopenia in older obese women. In 307 Caucasian women (71 ± 6 years, 66.3 ± 11.3 kg), skeletal muscle mass was estimated using bioelectric impedance, and function was tested with a 30 s one-leg standing-balance test. Biceps brachii thickness and vastus lateralis cross-sectional area (VLACSA) were measured with B-mode ultrasonography. Handgrip strength, maximum voluntary contraction elbow flexion (MVCEF), and knee extension torque (MVCKE) were measured by dynamometry, and MVCKE/VLACSA was calculated. Genotyping was performed for 24 single-nucleotide polymorphisms (SNPs), selected based on their previous associations with muscle-related phenotypes. Based on sarcopenia and obesity thresholds, groups were classified as sarcopenic obese, non-sarcopenic obese, sarcopenic non-obese, or non-sarcopenic non-obese. A two-way analysis of covariance was used to assess the main effects of sarcopenia and obesity on muscle-related phenotypes and binary logistic regression was performed for each SNP to investigate associations with sarcopenia in obesity. There were no significant obesity * sarcopenic status interactions for any of the investigated muscle-related phenotypic parameters. Neither sarcopenia nor obesity had a significant effect on biceps brachii thickness, but sarcopenia was associated with lower VLACSA (p = 0.003). Obesity was associated with lower MVCEF (p = 0.032), MVCKE (p = 0.047), and MVCKE/VLACSA (p = 0.012) with no significant effect of sarcopenia. Adjusted for age and height, three SNPs (ACTN3 rs1815739, MTHFR rs1801131, and MTHFR rs1537516) were associated with sarcopenia in obese participants. Sarcopenia was associated with a smaller muscle size, while obesity resulted in a lower muscle quality irrespective of sarcopenia. Three gene variants (ACTN3 rs1815739, MTHFR rs1801131, and MTHFR rs1537516) suspected to affect muscle function, homocysteine metabolism, or DNA methylation, respectively, were associated with sarcopenia in obese elderly women. Understanding the skeletal muscle features affected by sarcopenia and obesity, and identification of genes related to sarcopenia in obese women, may facilitate early detection of individuals at particular risk of sarcopenic obesity.
Numerical Simulation of Cavitation and Damping Force Characteristics for a High-Speed Supercavitation Vehicle
Authors: Lu Rui , Pan Guang , Tan Kun , Yin Shaoping
Published: October 2021

In this study, an attempt has been made to investigate the supercavitation and hydrodynamic characteristics of high-speed vehicles. A homogeneous equilibrium flow model and a Schnerr–Sauer model based on the Reynolds-averaged Navier–Stokes method are used. Grid-independent inspection and comparison with experimental data in the literature have been carried out to verify the accuracy of numerical methods. The effect of the navigation speed and angle of attack on the cavitation morphology and dynamic characteristics has been investigated. It has been demonstrated that the angle of attack has a remarkable influence on the wet surface and hydrodynamic force, whereas navigation speed has little effect on the position force of the vehicle under the circumstance of no wet surface. The hydrodynamic force changes periodically with the swing of the vehicle, but its maximum is greater than that for the direct navigation state at the same attack angle. Moreover, the damping effect obviously affects the hydrodynamic force amplitude and movement trend.
Machine Learning-Based Classification of Dependence in Ambulation in Stroke Patients Using Smartphone Video Data
Authors: Lee Jong Taek , Park Eunhee , Jung Tae-Du
Published: October 2021

The goal of this study was to develop a framework to classify dependence in ambulation by employing a deep model in a 3D convolutional neural network (3D-CNN) using video data recorded by a smartphone during inpatient rehabilitation therapy in stroke patients. Among 2311 video clips, 1218 walk action cases were collected from 206 stroke patients receiving inpatient rehabilitation therapy (63.24 ± 14.36 years old). As ground truth, the dependence in ambulation was assessed and labeled using the functional ambulatory categories (FACs) and Berg balance scale (BBS). The dependent ambulation was defined as a FAC score less than 4 or a BBS score less than 45. We extracted patient-centered video and patient-centered pose of the target from the tracked target’s posture keypoint location information. Then, the extracted patient-centered video was input in the 3D-CNN, and the extracted patient-centered pose was used to measure swing time asymmetry. Finally, we evaluated the classification of dependence in ambulation using video data via fivefold cross-validation. When training the 3D-CNN based on FACs and BBS, the model performed with 86.3% accuracy, 87.4% precision, 94.0% recall, and 90.5% F1 score. When the 3D-CNN based on FACs and BBS was combined with swing time asymmetry, the model exhibited improved performance (88.7% accuracy, 89.1% precision, 95.7% recall, and 92.2% F1 score). The proposed framework for dependence in ambulation can be useful, as it alerts clinicians or caregivers when stroke patients with dependent ambulatory move alone without assistance. In addition, monitoring dependence in ambulation can facilitate the design of individualized rehabilitation strategies for stroke patients with impaired mobility and balance function.
Current Overview on the Use of Mesenchymal Stem Cells for Perianal Fistula Treatment in Patients with Crohn’s Disease
Authors: Włodarczyk Marcin , Czerwińska Katarzyna , Włodarczyk Jakub , Fichna Jakub , Dziki Adam , Dziki Łukasz
Published: October 2021

Perianal fistula in patients with Crohn’s disease is an extremely challenging condition. The disease tends to reoccur, and with current treatment options, a large number of patients are left with active ailment and experience major morbidity. In recent years, hopeful results regarding local use of mesenchymal stem cells (MSCs) in perianal Crohn’s disease have been published. Although to this day there are no clear guidelines determining optimal dosage, injections frequency and culture conditions, their efficiency has proven to be much higher than conventionally used methods. According to studies, they can effectively induce as well as maintain fistula closure. This approach also avoids common side effects related to conventional surgical treatment.
A Max-Margin Model for Predicting Residue—Base Contacts in Protein–RNA Interactions
Authors: Kashiwagi Shunya , Sato Kengo , Sakakibara Yasubumi
Published: October 2021

Protein–RNA interactions (PRIs) are essential for many biological processes, so understanding aspects of the sequences and structures involved in PRIs is important for unraveling such processes. Because of the expensive and time-consuming techniques required for experimental determination of complex protein–RNA structures, various computational methods have been developed to predict PRIs. However, most of these methods focus on predicting only RNA-binding regions in proteins or only protein-binding motifs in RNA. Methods for predicting entire residue–base contacts in PRIs have not yet achieved sufficient accuracy. Furthermore, some of these methods require the identification of 3D structures or homologous sequences, which are not available for all protein and RNA sequences. Here, we propose a prediction method for predicting residue–base contacts between proteins and RNAs using only sequence information and structural information predicted from sequences. The method can be applied to any protein–RNA pair, even when rich information such as its 3D structure, is not available. In this method, residue–base contact prediction is formalized as an integer programming problem. We predict a residue–base contact map that maximizes a scoring function based on sequence-based features such as k-mers of sequences and the predicted secondary structure. The scoring function is trained using a max-margin framework from known PRIs with 3D structures. To verify our method, we conducted several computational experiments. The results suggest that our method, which is based on only sequence information, is comparable with RNA-binding residue prediction methods based on known binding data.
Dislocation Topological Evolution and Energy Analysis in Misfit Hardening of Spherical Precipitate by the Parametric Dislocation Dynamics Simulation
Authors: Zheng Haiwei , Liu Jianbin , Muraishi Shinji
Published: October 2021

Interaction of a single dislocation line and a misfit spherical precipitate has been simulated by the Parametric Dislocation Dynamics (PDD) method in this research. The internal stress inside the precipitate is deduced from Eshelby’s inclusion theory, the stress of the dislocation line and outside the precipitate is calculated by Green’s function. The influence of different relative heights of the primary slip plane on dislocation evolution is investigated, while the cross-slip mechanism and annihilation reaction are considered. The simulation results show three kinds of dislocation topological evolution: loop-forming (Orowan loop or prismatic loop), helix-forming, and gradual unpinning. The dislocation nodal force and the velocity vectors are visualized to study dislocation motion tendency. According to the stress–strain curve and the energy curves associated with the dislocation motion, the pinning stress level is strongly influenced by the topological change of dislocation as well as the relative heights of the primary slip plane.
Single-Point Incremental Forming of Titanium and Titanium Alloy Sheets
Authors: Oleksik Valentin , Trzepieciński Tomasz , Szpunar Marcin , Chodoła Łukasz , Ficek Daniel , Szczęsny Ireneusz
Published: October 2021

Incremental sheet forming of titanium and its alloys has a significant role in modern manufacturing techniques because it allows for the production of high-quality products with complex shapes at low production costs. Stamping processes are a major contributor to plastic working techniques in industries such as automotive, aerospace and medicine. This article reviews the development of the single-point incremental forming (SPIF) technique in titanium and its alloys. Problems of a tribological and microstructural nature that make it difficult to obtain components with the desired geometric and shape accuracy are discussed. Great emphasis is placed on current trends in SPIF of difficult-to-form α-, α + β- and β-type titanium alloys. Potential uses of SPIF for forming products in various industries are also indicated, with a particular focus on medical applications. The conclusions of the review provide a structured guideline for scientists and practitioners working on incremental forming of titanium and titanium alloy sheets. One of the ways to increase the formability and minimize the springback of titanium alloys is to treat them at elevated temperatures. The main approaches developed for introducing temperature into a workpiece are friction heating, electrical heating and laser heating. The selection of an appropriate lubricant is a key aspect of the forming process of titanium and its alloys, which exhibit unfavorable tribological properties such as high adhesion and a tendency to adhesive wear. A review of the literature showed that there are insufficient investigations into the synergistic effect of rotational speed and tool rotation direction on the surface roughness of workpieces.
Statistical Inference of Left Truncated and Right Censored Data from Marshall–Olkin Bivariate Rayleigh Distribution
Authors: Wu Ke , Wang Liang , Yan Li , Lio Yuhlong
Published: October 2021

In this paper, statistical inference and prediction issue of left truncated and right censored dependent competing risk data are studied. When the latent lifetime is distributed by Marshall–Olkin bivariate Rayleigh distribution, the maximum likelihood estimates of unknown parameters are established, and corresponding approximate confidence intervals are also constructed by using a Fisher information matrix and asymptotic approximate theory. Furthermore, Bayesian estimates and associated high posterior density credible intervals of unknown parameters are provided based on general flexible priors. In addition, when there is an order restriction between unknown parameters, the point and interval estimates based on classical and Bayesian frameworks are discussed too. Besides, the prediction issue of a censored sample is addressed based on both likelihood and Bayesian methods. Finally, extensive simulation studies are conducted to investigate the performance of the proposed methods, and two real-life examples are presented for illustration purposes.