bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Objective Optimization Research on the Integration of Renewable Energy HVAC Systems Based on TRNSYS
Authors: Si Qiang , Peng Yougang , Jin Qiuli , Li Yuan , Cai Hao
Published: December 2023

Well-designed passive buildings can drastically reduce building energy consumption, and optimal design of air conditioning systems is the key to achieving low operating energy consumption in near-zero energy buildings. TRNSYS was used to build the simulation model for a near-zero-energy building and its air conditioning system in Beijing. The Taguchi method was used to sort the design parameters that affect system performance according to the degree of influence and find the best combination of design parameters to optimize the system, which increased the solar fraction of the system by 4.6% and reduced the annual operating energy consumption by 7.32%. For the optimized system, a multi-objective optimization function of the life cycle costs and carbon emissions was established. By comparing the energy consumption, life cycle costs, and carbon emissions of the air conditioning system under different system configurations, optimal configuration solutions under different design target weights were obtained. It was found that using a ground source heat pump system + solar collector system had better energy-savings benefits, but the operating costs were slightly higher. The application of absorption refrigeration can reduce the system operating costs but will increase the initial investment. The best economic benefits were achieved using the ground source heat pump system + solar collector system for heating in winter and the ground source heat pump system for cooling in summer, and the best environmental benefits were obtaining using the ground source heat pump system + solar collector system for heating in winter and the ground source heat pump system + solar absorption refrigeration system in summer, which provides a reference for the optimization design and research of air conditioning systems in near-zero energy buildings.
The Impact of BRAF Mutation Status on Survival Outcomes and Treatment Patterns among Metastatic Colorectal Cancer Patients in Alberta, Canada
Authors: Sutherland R. Liam , Boyne Devon J. , Brenner Darren R. , Cheung Winson Y.
Published: December 2023

Colorectal cancer presents via multiple different clinical phenotypes that can arise from a variety of different genetic and molecular alterations. The aim of this study was to describe survival outcomes and treatment patterns of metastatic colorectal cancer (mCRC) patients by v-raf murine sarcoma viral oncogene homolog B1 (BRAF) mutation status. The Alberta Cancer Registry was used to identify all patients >18 years old who had been diagnosed with mCRC in Alberta between 1 January 2017 and 31 December 2019 and had received at least one cycle of systemic therapy. Treatment patterns were compared between wild-type and mutant BRAF mCRC patients. Cox regression models and Kaplan–Meier curves were created to assess survival differences by both treatment pattern and BRAF status. A total of 488 patients were identified with mCRC, of which 42 (11.4%) were confirmed to have a BRAF mutation. The most common first-line treatment regimen was either capecitabine and oxaliplatin (CAPOX) or leucovorin calcium (folinic acid), fluorouracil, and oxaliplatin (FOLFOX). The median overall survival for mCRC patients was 20.01 months. Mutant BRAF patients had a median survival of 8.21 months compared to 20.03 months among those with wild-type BRAF. BRAF mutations among mCRC patients are associated with a considerably poor prognosis, reinforcing the need for clinical BRAF testing among newly diagnosed patients to better understand their prognosis.
New Insights on Liver-Directed Therapies in Hepatocellular Carcinoma
Authors: Dalzell Christina G. , Taylor Amy C. , White Sarah B.
Published: December 2023

The incidence of hepatocellular carcinoma (HCC) has been increasing over the past decades, but improvements in systemic and locoregional therapies is increasing survival. Current locoregional treatment options include ablation, transarterial chemoembolization (TACE), transarterial radioembolization (TARE), and stereotactic body radiotherapy (SBRT). There is ongoing research regarding the combination of systemic and local therapies to maximize treatment effect as well as in new non-invasive, image-guided techniques such as histotripsy. There is also active research in optimizing the delivery of therapy to tumors via nanostructures and viral-vector-mediated gene therapies. In many cases, patients require a combination of therapies to achieve tumor control and prolong survival. This article provides an overview of the most common liver-directed therapies for HCC as well as insight into more recent advances in personalized medicine and emerging techniques.
A Review of Advanced Cutaneous Melanoma Therapies and Their Mechanisms, from Immunotherapies to Lysine Histone Methyl Transferase Inhibitors
Authors: de Oliveira Filho Renato Santos , de Oliveira Daniel Arcuschin , Nisimoto Melissa Maeda , Marti Luciana Cavalheiro
Published: December 2023

Advanced cutaneous melanoma is considered to be the most aggressive type of skin cancer and has variable rates of treatment response. Currently, there are some classes of immunotherapy and target therapies for its treatment. Immunotherapy can inhibit tumor growth and its recurrence by triggering the host’s immune system, whereas targeted therapy inhibits specific molecules or signaling pathways. However, melanoma responses to these treatments are highly heterogeneous, and patients can develop resistance. Epigenomics (DNA/histone modifications) contribute to cancer initiation and progression. Epigenetic alterations are divided into four levels of gene expression regulation: DNA methylation, histone modification, chromatin remodeling, and non-coding RNA regulation. Deregulation of lysine methyltransferase enzymes is associated with tumor initiation, invasion, development of metastases, changes in the immune microenvironment, and drug resistance. The study of lysine histone methyltransferase (KMT) and nicotinamide N-methyltransferase (NNMT) inhibitors is important for understanding cancer epigenetic mechanisms and biological processes. In addition to immunotherapy and target therapy, the research and development of KMT and NNMT inhibitors is ongoing. Many studies are exploring the therapeutic implications and possible side effects of these compounds, in addition to their adjuvant potential to the approved current therapies. Importantly, as with any drug development, safety, efficacy, and specificity are crucial considerations when developing methyltransferase inhibitors for clinical applications. Thus, this review article presents the recently available therapies and those in development for advanced cutaneous melanoma therapy.
Prevalence of Variants of Uncertain Significance in Patients Undergoing Genetic Testing for Hereditary Breast and Ovarian Cancer and Lynch Syndrome
Authors: Chrysafi Pavlina , Jani Chinmay T. , Lotz Margaret , Al Omari Omar , Singh Harpreet , Stafford Katherine , Agarwal Lipisha , Rupal Arashdeep , Dar Abdul Qadir , Dangelo Abby , Lam Prudence
Published: December 2023

Hereditary Breast and Ovarian Cancer (HBOC) and Lynch Syndrome (LS) are the most common inherited cancer syndromes identified with genetic testing. Testing, though, commonly reveals variants of uncertain significance (VUSs). This is a retrospective observational study designed to determine the prevalence of pathogenic mutations and VUSs in patients tested for HBOC and/or LS and to explore the characteristics of the VUS population. Patients 18–80 years old that met NCCN criteria for HBOC and/or LS genetic screening were tested between 2006 and 2020 at Mount Auburn Hospital in Cambridge, Massachusetts. A total of 663 patients were included in the study, with a mean age of 50 years old and 90% being females. Pathogenic mutations were identified in 12.5% and VUSs in 28.3%. VUS prevalence was associated with race (p-value = 0.019), being particularly higher in Asian populations. Patients with a personal history of breast cancer or family history of breast or ovarian cancer were more likely to have a VUS (personal breast: OR: 1.55; CI: 1.08–2.25; family breast: OR: 1.68; CI: 1.08–2.60, family ovarian OR: 2.29; CI: 1.04–5.45). In conclusion, VUSs appear to be detected in almost one third patients tested for cancer genetic syndromes, and thus future work is warranted to determine their significance in cancer development.
Analgesic Effect of Low-Level Laser Therapy before Heel Lance for Pain Management in Healthy Term Neonates: A Randomized Controlled Trial
Authors: Wu Bei-Yu , Ou-Yang Mei-Chen , Liu Chun-Ting , Huang Hsin-Chun , Hu Wen-Long , Chen I-Lun , Chang Hsin-Yu , Chung Mei-Yung , Chen Feng-Shun , Chen Yung-Hsiang , Chen Chih-Cheng
Published: December 2023

Currently, the prevention, assessment, and management of procedural pain in neonates continues to challenge clinicians and researchers. Objective. To investigate the analgesic effect of low-level laser therapy (LLLT) during heel lance compared to breast milk (BM) feeding in healthy term neonates. In this randomized controlled trial, healthy term neonates who underwent heel lance were randomly assigned to an LLLT or a BM group. The LLLT group received laser therapy to the heel lance site for 20 s before heel lance. The BM group received 5 mL expressed BM via a syringe before heel lance. The primary outcomes were behavioral responses. The secondary outcomes were physiological responses and levels of salivary cortisol and α-amylase. A total of 125 neonates were included, of whom 55 in the LLLT group and 59 in the BM group completed the study. There were no significant differences in latency to first cry and cry duration between the two groups. The squeeze time was significantly shorter in the LLLT group than in the BM group (p = 0.047). There were no significant differences in pain scores, heart rate, respiratory rate, oxygen saturation, and blood pressure before and after heel lance between the two groups. There were no significant differences in salivary cortisol and α-amylase levels in the LLLT group before and after heel lance; however, the differences were significant in the BM group. These findings suggest that the analgesic effect of LLLT is similar to that of BM during heel lance in healthy term neonates. LLLT has potential as an analgesic treatment.
Dissociating Autoantibody Responses against Ro52 Antigen in Patients with Anti-Synthetase or Anti-MDA5 Antibodies
Authors: Yoshida Akira , Nagata Shunya , Okazaki Yuka , Hanaoka Hironari , Gono Takahisa , Kuwana Masataka
Published: December 2023

We aimed to dissociate the autoantibody response against the Ro52 protein in patients with anti-synthetase or anti-melanoma differentiation-associated gene 5 (MDA5) antibodies to explore the potential roles of different anti-Ro52 autoantibody responses in disease subclassification. This study used a single-center, prospective myositis cohort involving 122 consecutive patients with anti-synthetase antibodies identified by RNA immunoprecipitation (RNA-IP) and 34 patients with anti-MDA5 antibodies detected using enzyme immunoassay (EIA). Anti-Ro52 antibodies were measured using commercial EIA kits, while anti-Ro/SSA antibodies were identified using RNA-IP. Clinical features and outcomes were stratified according to two different patterns of autoantibody responses against Ro52, including “isolated anti-Ro52”, defined by positive anti-Ro52 and negative anti-Ro/SSA antibodies, and “anti-SSA-Ro52”, defined by positive anti-Ro52 and anti-Ro/SSA antibodies. Isolated anti-Ro52 positivity was the most prevalent autoantibody response in patients with both anti-synthetase (40/122; 32.8%) and anti-MDA5 antibodies (8/34; 23.5%). Isolated anti-Ro52 or anti-SSA-Ro52 positivity was associated with Gottron’s sign in patients with anti-synthetase antibodies, while in patients with anti-MDA5 antibodies, isolated anti-Ro52 positivity was associated with respiratory insufficiency at initial presentation and poor overall survival. Isolated anti-Ro52 positivity could be a potential biomarker for patient stratification; however, the clinical significance of dissociating isolated anti-Ro52 positivity from overall anti-Ro52 positivity was not evident.
Acute Colonic Diverticulitis: CT Findings, Classifications, and a Proposal of a Structured Reporting Template
Authors: Tiralongo Francesco , Di Pietro Stefano , Milazzo Dario , Galioto Sebastiano , Castiglione Davide Giuseppe , Ini’ Corrado , Foti Pietro Valerio , Mosconi Cristina , Giurazza Francesco , Venturini Massimo , Zanghi’ Guido Nicola , Palmucci Stefano , Basile Antonio
Published: December 2023

Acute colonic diverticulitis (ACD) is the most common complication of diverticular disease and represents an abdominal emergency. It includes a variety of conditions, extending from localized diverticular inflammation to fecal peritonitis, hence the importance of an accurate diagnosis. Contrast-enhanced computed tomography (CE-CT) plays a pivotal role in the diagnosis due to its high sensitivity, specificity, accuracy, and interobserver agreement. In fact, CE-CT allows alternative diagnoses to be excluded, the inflamed diverticulum to be localized, and complications to be identified. Imaging findings have been reviewed, dividing them into bowel and extra-intestinal wall findings. Moreover, CE-CT allows staging of the disease; the most used classifications of ACD severity are Hinchey’s modified and WSES classifications. Differential diagnoses include colon carcinoma, epiploic appendagitis, ischemic colitis, appendicitis, infectious enterocolitis, and inflammatory bowel disease. We propose a structured reporting template to standardize the terminology and improve communication between specialists involved in patient care.
Teacher Morale and Mental Health at the Conclusion of the COVID-19 Pandemic
Authors: Pressley Tim , Marshall David T. , Love Savanna M. , Neugebauer Natalie M.
Published: December 2023

As teachers entered the 2021–2022 school year, schools tasked teachers with the challenge of closing the learning loss gap, maintaining the same standards as a typical school year, implementing COVID-19 safety protocols and teaching to grade-level standards. The current study used the theoretical framework of teacher demoralization to explore teachers’ experiences of morale and mental health at the end of the 2021–2022 school year. The participants included 830 PK-12 individual teachers from across the United States who completed an online survey. The data were analysed using Pearson’s correlation and the hierarchical multiple regression model to determine the predictor variables. The results showed significant predictors for both teacher morale and mental health. The implications focus on steps that school leaders can take to support teachers’ morale and mental health.
Daily Peak-Valley Electric-Load Forecasting Based on an SSA-LSTM-RF Algorithm
Authors: Wang Yaoying , Sun Shudong , Cai Zhiqiang
Published: December 2023

In recent years, with the development of societies and economies, the demand for social electricity has further increased. The efficiency and accuracy of electric-load forecasting is an important guarantee for the safety and reliability of power system operation. With the sparrow search algorithm (SSA), long short-term memory (LSTM), and random forest (RF), this research proposes an SSA-LSTM-RF daily peak-valley forecasting model. First, this research uses the Pearson correlation coefficient and the random forest model to select features. Second, the forecasting model takes the target value, climate characteristics, time series characteristics, and historical trend characteristics as input to the LSTM network to obtain the daily-load peak and valley values. Third, the super parameters of the LSTM network are optimized by the SSA algorithm and the global optimal solution is obtained. Finally, the forecasted peak and valley values are input into the random forest as features to obtain the output of the peak-valley time. The forest value of the SSA-LSTM-RF model is good, and the fitting ability is also good. Through experimental comparison, it can be seen that the electric-load forecasting algorithm based on the SSA-LSTM-RF model has higher forecasting accuracy and provides ideal performance for electric-load forecasting with different time steps.
Numerical Investigation of the Thermal Performance of a Hybrid Phase Change Material and Forced Air Cooling System for a Three-Cell Lithium-Ion Battery Module
Authors: Huynh Van-Tinh , Chang Kyoungsik , Lee Sang-Wook
Published: December 2023

The thermal performance of a lithium-ion battery module comprising three cells contained within a casing was investigated at discharge rates of 3C and 5C with three different cooling strategies: forced air, phase-change material (PCM), and a hybrid system using a combination of forced air and the PCM. Three levels of fan speed (5000 rpm; 7000 rpm; and 9000 rpm) for cooling air flow were considered. A numerical simulation of heat transfer was performed using the ANSYS Fluent software. The electrochemical modelling of a battery was developed based on the NTGK approach, and the phase-change phenomenon was treated as an enthesis–porosity problem. The composite PCM, aluminum metal foam embedded in n-octadecane, had better heat dissipation performance than forced air convection. The PCM is significantly more effective at heat dissipation than forced air. Interestingly, when using a hybrid cooling system that combines forced air and a PCM, although it meets the operational requirements for Li-ion batteries in regard to maximum temperature and temperature uniformity at a 3C discharge rate, the airflow appears to have a negligible effect on thermal management and yields an indiscernible change in temperature. This can be attributed to a complex flow pattern that developed in a casing as a result of the suboptimal design of the inlet and outlet. Further studies will be required for the optimal positioning of the inlet and outlet, as well as the effectiveness of combining liquid cooling methods.
Using Ground Penetrating Radar (GPR) to Predict Log Moisture Content of Commercially Important Canadian Softwoods
Authors: Duchesne Isabelle , Tong Queju , Hans Guillaume
Published: December 2023

The non-destructive testing of wood fibre properties is crucial for informing forest management decisions and achieving optimal resource utilization. Moisture content (MC) is an important indicator of wood freshness and may reveal the presence of wood degradation. However, efficient methods are still needed to better monitor this property along the forest–wood value chain. The objective of the study was to develop prediction models to evaluate log MC based on the propagation of ground penetrating radar (GPR) signals. A total of 165 trees representing four species (black spruce (Picea mariana (Mill.) B.S.P.), white spruce (Picea glauca (Moench) Voss), red spruce (Picea rubens Sarg.), and balsam fir (Abies balsamea (L.) Mill.)) were harvested in two regions of the province of Quebec. GPR signals were acquired in the green (fresh) state and at three subsequent drying stages. Partial least squares regression (PLSR) and locally weighted PLSR (LWPLSR) were employed to establish relationships between GPR signals (antenna frequency: 1.6 GHz) and log properties. The models were fitted on three calibration sets containing four drying stages and different species mixes. The LWPLSR models performed better than the PLSR models for predicting log MC, with a lower root mean square error (RMSEp range: 10.8%–20.2% vs. 13.0%–20.5%) and a higher R2p (0.63–0.87 vs. 0.62–0.82). Spruce-only models performed considerably better than fir-only models while multi-species models were in-between. Despite the complex anisotropy of wood and the physics of wave propagation, the GPR technology can be successfully used to estimate log moisture content, but the GPR-based MC models should be calibrated for each specific type of wood material.
S0 Galaxies: Outer Gas Accretion through Tidal Interaction and Minor Merging
Authors: Sil’chenko Olga , Moiseev Alexei V. , Smirnova Alexandrina , Uklein Roman
Published: December 2023

To clarify the sources of outer gas accretion onto disk galaxies, we study the vicinity of four interacting galaxy systems in the Hα emission line by using the scanning Fabry–Perot interferometer of the 6m telescope of the Special Astrophysical Observatory RAS. We find perspective accretion flows seen as ionized-gas emission filaments between the galaxies. We discuss the whole kinematics and origin of these flows.
Apsara Aesthetics and Belonging: On Mixed-Race Cambodian American Performance
Authors: Lytle Tiffany J.
Published: December 2023

The image of the Apsara, a celestial dancer in Cambodian myth, is closely associated with Cambodian cultural preservation practices like Cambodian classical dance. The Apsara, its aesthetic features and its association with Cambodian cultural preservation have taken on new meaning in Cambodia’s diasporic communities. In the diaspora, Apsara aesthetics have come to symbolize Cambodian heritage, history and identity, becoming a major feature of performances by Cambodian diasporic artists. However, orientalist expectations of Asian performers in the diaspora, paired with both the forgotten history of colonial intervention in Cambodian arts and state-sanctioned initiatives towards Cambodian nationalism, contributes to orientalist (and thus racialized) expectations of Cambodian diasporic performance. Mixed-race artists fail to fit neatly into the dominant narratives of Cambodian performance and have been marginalized by the Cambodian diasporic community’s dominant conceptions of performance that are rooted in cultural preservation. As people that sit outside of the aestheticized markers of Cambodian-ness, mixed-race artists often struggle to have their work and their subjectivities recognized by their communities. To circumvent questions of their racial legibility, mixed-race Cambodian American artists construct performances that are strategically padded with markers of Khmer identity by engaging with Apsara aesthetics. This article will explore how three different SoCal-based artists have negotiated their Cambodian American identity and cultural politics through performance and/or performance related materials (ads, images, etc.). I will be using examples from the work of music artist and violinist Chrysanthe Tan, theater practitioner Kalean Ung, and autoethnographic engagement with my own creative projects to show how examining the work of multi-racial Cambodian American performing artists can bring forth the complex dynamics of Cambodian diasporic cultural politics and belonging.
Transcriptome Profiling after Early Spinal Cord Injury in the Axolotl and Its Comparison with Rodent Animal Models through RNA-Seq Data Analysis
Authors: González-Orozco Juan Carlos , Escobedo-Avila Itzel , Velasco Iván
Published: December 2023

Background: Traumatic spinal cord injury (SCI) is a disabling condition that affects millions of people around the world. Currently, no clinical treatment can restore spinal cord function. Comparison of molecular responses in regenerating to non-regenerating vertebrates can shed light on neural restoration. The axolotl (Ambystoma mexicanum) is an amphibian that regenerates regions of the brain or spinal cord after damage. Methods: In this study, we compared the transcriptomes after SCI at acute (1–2 days after SCI) and sub-acute (6–7 days post-SCI) periods through the analysis of RNA-seq public datasets from axolotl and non-regenerating rodents. Results: Genes related to wound healing and immune responses were upregulated in axolotls, rats, and mice after SCI; however, the immune-related processes were more prevalent in rodents. In the acute phase of SCI in the axolotl, the molecular pathways and genes associated with early development were upregulated, while processes related to neuronal function were downregulated. Importantly, the downregulation of processes related to sensorial and motor functions was observed only in rodents. This analysis also revealed that genes related to pluripotency, cytoskeleton rearrangement, and transposable elements (e.g., Sox2, Krt5, and LOC100130764) were among the most upregulated in the axolotl. Finally, gene regulatory networks in axolotls revealed the early activation of genes related to neurogenesis, including Atf3/4 and Foxa2. Conclusions: Immune-related processes are upregulated shortly after SCI in axolotls and rodents; however, a strong immune response is more noticeable in rodents. Genes related to early development and neurogenesis are upregulated beginning in the acute stage of SCI in axolotls, while the loss of motor and sensory functions is detected only in rodents during the sub-acute period of SCI. The approach employed in this study might be useful for designing and establishing regenerative therapies after SCI in mammals, including humans.
Quality of Life, Disability Level, and Pain Intensity among Patients after Lumbar Disc Surgery: An Observational Three-Month Follow-Up Study
Authors: Michalak Monika , Druszcz Adam , Miś Maciej , Paprocka-Borowicz Małgorzata , Rosińczuk Joanna
Published: December 2023

The prevalence of intervertebral disc degeneration in the lumbar region resulting in low back pain is high. One of the treatment options is neurosurgery. Previous studies and systematic reviews demonstrate the need to identify factors that affect the health-related quality of life of patients undergoing surgery. This study aimed to analyze the sociodemographic and clinical factors that affect the quality of life of patients undergoing lumbar disc surgery. A group of 128 patients was assessed for eligibility and qualified by radiological examinations for lumbar disc surgery by a neurosurgeon in the outpatient clinic. Finally, 110 patients were studied and evaluated 24 h and 3 months after surgery. Health-related quality of life (36-Item Short Form Survey, SF-36), disability level (Oswestry Disability Index, ODI), and pain intensity (Visual Analogue Scale, VAS) were assessed. The mean pain intensity before surgery was 7.8 ± 2.3 pts and decreased significantly 24 h after surgery, with a mean score of 3.8 ± 2.4 pts (p = 0.0000). After three months, the increase in pain intensity was at 4.8 ± 2.4 pts, but the score was still significantly better than before surgery (p = 0.0024). The mean ODI score before surgery was 29.3 ± 8.4 pts (slight disability), and three months after surgery, there was an insignificant increase to a mean value of 31.5 ± 10.4 pts (p = 0.0834). There was a statistically significant increase in quality-of-life scores at three months after surgery in the following domains: physical functioning (8.7%; p = 0.0176), bodily pain (26.2%; p = 0.0000), vitality (5.8%; p = 0.0132) and mental health (6.2%; p = 0.0163), and a decrease in role limitations due to physical problems (3.8; p = 0.0013) and general health perception (6.7%; p = 0.0112). In conclusion, the surgical procedure plays an important role in improving the quality of life of patients operated on for intervertebral disc degeneration. It was effective in reducing the pain level, especially 24 h after surgery; however, surgery did not affect the disability level.
Comment on Barczyk et al. Potential Clinical Applications of Ozone Therapy in Dental Specialties—A Literature Review, Supported by Own Observations. Int. J. Environ. Res. Public Health 2023, 20, 2048
Authors: Tricarico Gerardo , Travagli Valter
Published: December 2023

A review from Barczyk et al. has just appeared online [...]
Prolonged Disability following Re-Exposure after Complete Recovery from Aerotoxic Syndrome: A Case Report
Authors: Creeden Race , Blonien Nick , Schultz Jill K. , Wheeler Jessika , Haltson Erik L. , McKinney Zeke J.
Published: December 2023

Aerotoxic syndrome is a rarely recognized and poorly defined illness resulting from exposure to products of oil combustion in commercial aircraft bleed air systems. The pathophysiology of this syndrome is not well understood. A 42-year-old male pilot developed duty-limiting symptoms consistent with aerotoxic syndrome following exposure to airline cabin fumes and recurrence of symptoms following re-exposure despite apparent full recovery from the initial exposure event. Given his persistent symptoms and concern for immediate debilitation/incapacitation if re-exposed, the pilot was deemed medically unqualified for return to commercial piloting duties and a disability determination was made. Consideration of aerotoxic syndrome in aircrew members with unusual or episodic symptoms of neuro/cognitive dysfunction is essential for further characterizing this occupational illness, guiding medical management, and understanding its impact on aviation workforce health.
Social Support and Depression among Stroke Patients: A Topical Review
Authors: Zhou Haoyu , Kulick Erin R.
Published: December 2023

Research has shown a protective association between social support and depression, depression among stroke patients, and health impacts of depression. Despite this, not much is known about the effect of social support on depression among stroke patients. This review aims to summarize the current research examining the association between social support and depression among stroke patients. A literature search was performed in PubMed to find original peer-reviewed journal articles from 2016 to 12 March 2023 that examined the association between social support and depression among stroke patients. The search terms were depression and “social support” and stroke, which lead to 172 articles. After abstract review, seven observational studies that studied the target association among stroke patients were selected. One additional study was found using PsycINFO as a complementary source with the same search strategy and criteria. Overall, a negative association was found between social support and depression among stroke patients in eight studies, with more social support leading to lower rates of depression post-stroke. The other study did not find a statistically significant association. Overall, the results of recent studies suggest that social support is negatively associated with depression among stroke patients. In most studies, this association was statistically significant. The findings suggest the importance of improving social support perceived by stroke patients in the prevention of depression after the occurrence of stroke.
Brassinosteroid Signaling Pathways: Insights into Plant Responses under Abiotic Stress
Authors: Khan Tanveer Alam , Kappachery Sajeesh , Karumannil Sameera , AlHosani Mohamed , Almansoori Nemah , Almansoori Hamda , Yusuf Mohammad , Tran Lam-Son Phan , Gururani Mayank Anand
Published: December 2023

With the growing global population, abiotic factors have emerged as a formidable threat to agricultural food production. If left unaddressed, these stress factors might reduce food yields by up to 25% by 2050. Plants utilize natural mechanisms, such as reactive oxygen species scavenging, to mitigate the adverse impacts of abiotic stressors. Diverse plants exhibit unique adaptations to abiotic stresses, which are regulated by phytohormones at various levels. Brassinosteroids (BRs) play a crucial role in controlling essential physiological processes in plants, including seed germination, xylem differentiation, and reproduction. The BR cascade serves as the mechanism through which plants respond to environmental stimuli, including drought and extreme temperatures. Despite two decades of research, the complex signaling of BRs under different stress conditions is still being elucidated. Manipulating BR signaling, biosynthesis, or perception holds promise for enhancing crop resilience. This review explores the role of BRs in signaling cascades and summarizes their substantial contribution to plants’ ability to withstand abiotic stresses.
The Development of Human Ex Vivo Models of Inflammatory Skin Conditions
Authors: Wang Eddy Hsi Chun , Barresi-Thornton Rebecca , Chen Li-Chi , Senna Maryanne Makredes , Liao I-Chien , Chen Ying , Zheng Qian , Bouez Charbel
Published: December 2023

Traditional research in inflammatory dermatoses has relied on animal models and reconstructed human epidermis to study these conditions. However, these models are limited in replicating the complexity of real human skin and reproducing the intricate pathological changes in skin barrier components and lipid profiles. To address this gap, we developed experimental models that mimic various human inflammatory skin phenotypes. Human ex vivo skins were stimulated with various triggers, creating models for inflammation-induced angiogenesis, irritation response, and chronic T-cell activation. We assessed the alterations in skin morphology, cellular infiltrates, cytokine production, and epidermal lipidomic profiles. In the pro-angiogenesis model, we observed increased mast cell degranulation and elevated levels of angiogenic growth factors. Both the irritant and chronic inflammation models exhibited severe epidermal disruption, along with macrophage infiltration, leukocyte exocytosis, and heightened cytokine levels. Lipidomic analysis revealed minor changes in the pro-angiogenesis model, whereas the chronic inflammation and irritant models exhibited significant decreases in barrier essential ceramide subclasses and a shift toward shorter acyl chain lengths (<C18), indicating skin barrier instability. Additionally, the irritant and chronic inflammation models are responsive to immunosuppressants. These models hold promise for advancing scientific understanding and the development of therapeutic and skincare solutions for individuals afflicted by compromised skin conditions.
Identification of a Gene Encoding a New Fungal Steroid 7-Hydroxylase and Its Functional Characterization in Pichia pastoris Yeast
Authors: Kollerov Vyacheslav , Tarlachkov Sergey , Shutov Andrei , Kazantsev Alexey , Donova Marina
Published: December 2023

The hydroxylation of steroids in the C7β position is one of the rare reactions that allow the production of value-added precursors in the synthesis of ursodeoxycholic acid and other pharmaceuticals. Recently, we discovered this activity in the ascomycete Curvularia sp. VKM F-3040. In this study, the novel gene of 7-hydroxylase (P450cur) was identified as being heterologously expressed and functionally characterized in Pichia pastoris. Transcriptome data mining and differential expression analysis revealed that 12 putative genes in Curvularia sp. mycelia significantly increased their expression in response to dehydroepiandrosterone (DHEA). The transcriptional level of the most up-regulated cytochrome P450cur gene was increased more than 300-fold. A two-gene construct with a candidate P450cur gene and the gene of its natural redox partner, NADPH-cytochrome P450 reductase (CPR), which is interconnected by a T2A element, was created. Using this construct, recombinant P. pastoris strains co-expressing fungal P450cur and CPR genes were obtained. The functional activity of the recombinant P450cur was studied in vivo during the bioconversion of androstane steroids. The fungal 7-monooxygenase predominantly catalyzed the 7β-hydroxylation of androstadienedione (ADD), DHEA, and androstenediol, whereas 1-dehydrotestosterone was hydroxylated by P450cur mainly at the C7-Hα position. To our knowledge, this is the first report of a recombinant yeast capable of catalyzing the 7α/β-hydroxylation of ADD and DHEA.
The Non-Invasive Prediction of Colorectal Neoplasia (NIPCON) Study 1995–2022: A Comparison of Guaiac-Based Fecal Occult Blood Test (FOBT) and an Anti-Adenoma Antibody, Adnab-9
Authors: Tobi Martin , Antaki Fadi , Rambus Mary Ann , Yang Yu-Xiao , Kaplan David , Rodriguez Rebecca , Maliakkal Benedict , Majumdar Adhip , Demian Ereny , Tobi Yosef Y. , Sochacki Paula , Ehrinpreis Murray , Lawson Michael G. , McVicker Benita
Published: December 2023

Given the need to improve the sensitivity of non-invasive methods to detect colorectal neoplasia, particularly adenomas, we compared a fecal test using a monoclonal antibody (Mab) raised against constituents of colonic adenomas designated Adnab-9 (Adenoma Antibody 9), recognizing an N-linked 87 kDa glycoprotein, to gFOBT, which is shown to reduce CRC mortality. p87 immunohistochemistry testing is significantly more sensitive (OR 3.64[CI 2.37–5.58]) than gFOBT (guaiac-based fecal occult blood test) for adenomas (<3 in number), advanced adenomas (OR 4.21[CI 2.47–7.15]), or a combination of the two (OR 3.35[CI 2.47–4.53]). p87 immunohistochemistry shows regional Paneth cell (PC) expression mainly in the right-sided colon and is significantly reduced in the ceca of African Americans (p < 0.0001). In a subset of patients, we obtained other body fluids such as urine, colonic effluent, and saliva. Urine tests (organ-specific neoantigen) showed a significant difference for advanced adenomas (p < 0.047). We conclude that fecal p87 testing is more sensitive than gFOBT and Adnab-9 and could be used to better direct the colonoscopy screening effort.
Cardioprotective Effects of the GRK2 Inhibitor Paroxetine on Isoproterenol-Induced Cardiac Remodeling by Modulating NF-κB Mediated Prohypertrophic and Profibrotic Gene Expression
Authors: Alonazi Asma S. , Bin Dayel Anfal F. , Albuaijan Danah A. , Bin Osfur Alhanouf S. , Hakami Fatemah M. , Alzayed Shaden S. , Almotairi Ahmad R. , Khan Mohammad R. , Alharbi Hana M. , Ali Rehab A. , Alamin Maha A. , Alghibiwi Hanan K. , Alrasheed Nouf M. , Alhosaini Khaled A.
Published: December 2023

Pathological cardiac remodeling is associated with cardiovascular disease and can lead to heart failure. Nuclear factor-kappa B (NF-κB) is upregulated in the hypertrophic heart. Moreover, the expression of the G-protein-coupled receptor kinase 2 (GRK2) is increased and linked to the progression of heart failure. The inhibitory effects of paroxetine on GRK2 have been established. However, its protective effect on IκBα/NFκB signaling has not been elucidated. This study investigated the cardioprotective effect of paroxetine in an animal model of cardiac hypertrophy (CH), focusing on its effect on GRK2-mediated NF-κB-regulated expression of prohypertrophic and profibrotic genes. Wistar albino rats were administered normal saline, paroxetine, or fluoxetine, followed by isoproterenol to induce CH. The cardioprotective effects of the treatments were determined by assessing cardiac injury, inflammatory biomarker levels, histopathological changes, and hypertrophic and fibrotic genes in cardiomyocytes. Paroxetine pre-treatment significantly decreased the HW/BW ratio (p < 0.001), and the expression of prohypertrophic and profibrotic genes Troponin-I (p < 0.001), BNP (p < 0.01), ANP (p < 0.001), hydroxyproline (p < 0.05), TGF-β1 (p < 0.05), and αSMA (p < 0.01) as well as inflammatory markers. It also markedly decreased pIκBα, NFκB(p105) subunit expression (p < 0.05) and phosphorylation. The findings suggest that paroxetine prevents pathological cardiac remodeling by inhibiting the GRK2-mediated IκBα/NF-κB signaling pathway.
High-Flow Tracheal Oxygen for Tracheostomy Tube Removal in Lung Transplant Recipients
Authors: Lionello Federico , Guarnieri Gabriella , Arcaro Giovanna , Bertagna De Marchi Leonardo , Molena Beatrice , Contessa Cristina , Boscolo Annalisa , Rea Federico , Navalesi Paolo , Vianello Andrea
Published: December 2023

(1) Background: Because of a complicated intraoperative course and/or poor recovery of graft function, approximately 15% of lung transplant (LT) recipients require prolonged mechanical ventilation (PMV) and receive a tracheostomy. This prospective study aimed to assess the effect of High-Flow Tracheal Oxygen (HFTO) on tracheostomy tube removal in LT recipients receiving PMV postoperatively. (2) Methods: The clinical course of 14 LT recipients receiving HFTO was prospectively evaluated and compared to that of 13 comparable controls receiving conventional oxygen therapy (COT) via tracheostomy. The study’s primary endpoint was the number of patients whose tracheostomy tube was removed at discharge from an Intermediate Respiratory Care Unit (IRCU). (3) Results: Setting up HFTO proved easy, and it was well tolerated by all the patients. The number of patients whose tracheostomy tube was removed was significantly higher in the HFOT group compared to the COT group [13/14 vs. 6/13 (p = 0.0128)]. (4) Conclusions: HFTO is an effective, safe therapy that facilitates tracheostomy tube removal in LT recipients after weaning from PMV.
Clinical Outcomes of Next-Generation Microwave Thermosphere Ablation for Hepatocellular Carcinoma with Primarily Hepatitis-Related Etiology
Authors: Nakamura Shinichiro , Tada Toshifumi , Sue Masahiko , Matsuo Yu , Murakami Shiho , Muramatsu Toshiro , Morii Kazuhiko , Okada Hiroyuki
Published: December 2023

Background and aim: We investigated the clinical outcomes of patients with hepatocellular carcinoma (HCC) who underwent next-generation microwave thermosphere ablation (MTA). Methods: A total of 429 patients with 607 HCCs (maximum tumor diameter ≤40 mm) were included. We defined the following areas of the liver as those where MTA therapy is difficult to perform: caudate lobe and areas near the primary and secondary branches of the intrahepatic portal vein, inferior vena cava, gallbladder, heart, duodenum, abdominal esophagus, collateral veins around the liver, and spleen. Factors which predisposed patients to local tumor recurrence in the context of tumor location and complications were examined. Results: The primary etiologies of HCC were hepatitis-related: 259 (60.4%) cases of HCV, 31 (7.3%) cases of HBV, and two instances of both. Median maximum tumor diameter was 15.0 (interquartile range, 10.0–21.0) mm. There were 86 tumors in areas of the liver where MTA is difficult. The most common area was near the primary and secondary branches of the intrahepatic portal vein (26 nodules). The cumulative local tumor recurrence rates at 1, 2, and 3 years were 4.4%, 8.0%, and 8.5%, respectively. The cumulative local tumor recurrence rate differed significantly by tumor size group: 6.6%, 13.8%, and 29.4% at three years in the ≤20 mm group (n = 483), 20–30 mm group (n = 107), and ≥30 mm group (n = 17), respectively (p < 0.001). The cumulative local tumor recurrence rate was similar despite difficult-to-treat status (p = 0.169). In the multivariable analysis, tumor size (>15 mm) (hazard ratio [HR], 2.15; 95% confidence interval [CI], 1.11–4.16; p = 0.023) and ablative margin (<3 mm) (HR, 2.94; 95% CI, 1.52–5.71; p = 0.001) were significantly associated with local tumor recurrence. Only tumor size (>15 mm) (odds ratio, 3.41 95% CI, 1.53–7.84; p = 0.026) was significantly associated with complications. Conclusions: MTA is a safe and effective local ablation therapy for HCC, even for tumors located in areas of the liver where local ablation therapy is difficult.
Real-Life Experience in the Efficacy and Safety of COVID-19 Vaccination in Patients with Advanced Cirrhosis
Authors: Hanafy Amr Shaaban , Embaby Ahmed , Salem Sara Mohamed , Behiry Ahmed , Ebrahim Hasnaa Ali , Elkattawy Hany Ahmed , Abed Sally Yussef , Almadani Moneer E. , El-Sherbiny Mohamad
Published: December 2023

COVID-19 infections accelerate liver decompensation and serious liver-related co-morbidities. The aim is to evaluate the safety and impact of COVID vaccines on hepatic disease progression in patients with advanced liver disease and to identify parameters that predict the occurrence of complications. The study involved 70 patients with advanced liver disease who were vaccinated with different COVID vaccines from January 2021 to April 2022. They were evaluated clinically. The laboratory investigation included a complete blood count, liver and kidney function tests, calculation of CTP and MELD scores, plasma levels of ammonia, abdominal ultrasound, and upper GI endoscopy. Twenty patients had experienced complications 64 ± 12 days from the last dose of a vaccination. Twenty patients (28.6%) developed hepatic decompensation and hypothyroidism (n = 11, 15.7%), and five (7.14%) patients developed splanchnic thrombosis. There were no COVID-19 reinfections except for two patients who received Sinopharm and developed vaccine-associated enhanced disease (2.9%). Complications after COVID vaccinations were correlated with ALT (r = 0.279, p = 0.019), serum sodium (r = −0.30, p = 0.005), creatinine (r = 0.303, p = 0.011), liver volume (LV) (r = −0.640, p = 0.000), and MELD score (r = 0.439, p = 0.000). Multivariate logistic regression revealed that LV is the only independent predictor (p = 0.001). LV ≤ 682.3 has a sensitivity of 95.24% and a specificity of 85.71% in predicting complications with an AUC of 0.935, p < 0.001. In conclusion, the hepatic reserve and prognosis in liver cirrhosis should be evaluated prior to COVID vaccinations using the MELD score and liver volume as promising risk stratification criteria. In summary, the research proposes a novel triaging strategy that involves utilizing the MELD score and liver volume as risk stratification parameters of the hepatic reserve and prognosis of advanced liver cirrhosis prior to COVID immunization to determine who should not receive a COVID vaccination.
Distinct Immunophenotypic Features in Patients Affected by 22q11.2 Deletion Syndrome with Immune Dysregulation and Infectious Phenotype
Authors: Costagliola Giorgio , Legitimo Annalisa , Bertini Veronica , Alberio Antonino Maria Quintilio , Valetto Angelo , Consolini Rita
Published: December 2023

The clinical expression of 22q11.2 deletion syndrome (22q11.2 DS) is extremely variable, as patients can present with recurrent or severe infections, immune dysregulation, atopic diseases, or extra-immunological manifestations. The immunological background underlying the different disease manifestations is not completely elucidated. The aim of this study was to identify the immunophenotypic peculiarities of 22q11.2 DS patients presenting with different disease expressions. This study included 34 patients with 22q11.2 DS, divided into three groups according to the clinical phenotype: isolated extra-immunological manifestations (G1), infectious phenotype with increased/severe infections (G2), and immune dysregulation (G3). The patients underwent extended immunophenotyping of the T and B lymphocytes and analysis of the circulating dendritic cells (DCs). In patients with an infectious phenotype, a significant reduction in CD3+ and CD4+ cells and an expansion of CD8 naïve cells was evidenced. On the other hand, the immunophenotype of the patients with immune dysregulation showed a skewing toward memory T cell populations, and reduced levels of recent thymic emigrants (RTEs), while the highest levels of RTEs were detected in the patients with isolated extra-immunological manifestations. This study integrates the current literature, contributing to elucidating the variability in the immune status of patients with 22q11.2DS with different phenotypic expressions, particularly in those with infectious phenotype and immune dysregulation.