bluuebunny/crossref_metadata_2025 huggingface dataset:
Unveiling a novel fusion gene enhances CAR T cell therapy for solid tumors
Authors: Zhou Zefeng , Xia Yongming , Chen Ruixiu , Gao Panpan , Duan Shiwei
Published: May 2024

The efficacy of Adoptive Cell Transfer Therapy (ACT) in combating hematological tumors has been well-documented, yet its application to solid tumors faces formidable hurdles, chief among them being the suboptimal therapeutic response and the immunosuppressive milieu within the tumor microenvironment (TME). Recently, Garcia, J. et al. present compelling findings shedding light on potential breakthroughs in this domain. Their investigation reveals the pronounced augmentation of anti-tumor activity in CAR T cells through the introduction of a T cell neoplasm fusion gene, CARD11-PIK3R3. The incorporation of this gene into engineered T cell therapy holds promise as a formidable tool in the arsenal of cancer immunotherapy. The innovative strategy outlined not only mitigates the requirement for high doses of CAR T cells but also enhances tumor control while exhibiting encouraging safety profiles. The exploration of the CARD11-PIK3R3 fusion gene represents an advancement in our approach to bolstering the anti-tumor efficacy of immunotherapeutic interventions. Nonetheless, the imperative for further inquiry to ascertain its transfection efficiency and long-term safety cannot be overstated. Nevertheless, this seminal investigation offers a beacon of hope in surmounting the formidable treatment impediments posed by solid tumors, paving the way for a transformative era in cancer therapeutics.
Quercetin promotes the proportion and maturation of NK cells by binding to MYH9 and improves cognitive functions in aged mice
Authors: Su Tingting , Shen Haitao , He Mengyuan , Yang Shanshan , Gong Xue , Huang Ce , Guo Liuling , Wang Hao , Feng Shengyu , Mi Taotao , Zhao Meili , Liu Qing , Huo Fengjiao , Zhu Jian-Kang , Zhu Jianbo , Li Hongbin , Liu Hailiang
Published: May 2024

Background

Quercetin is a flavonol compound widely distributed in plants that possesses diverse biological properties, including antioxidative, anti-inflammatory, anticancer, neuroprotective and senescent cell-clearing activities. It has been shown to effectively alleviate neurodegenerative diseases and enhance cognitive functions in various models. The immune system has been implicated in the regulation of brain function and cognitive abilities. However, it remains unclear whether quercetin enhances cognitive functions by interacting with the immune system.

Results

In this study, middle-aged female mice were administered quercetin via tail vein injection. Quercetin increased the proportion of NK cells, without affecting T or B cells, and improved cognitive performance. Depletion of NK cells significantly reduces cognitive ability in mice. RNA-seq analysis revealed that quercetin modulated the RNA profile of hippocampal tissues in aging animals towards a more youthful state. In vitro, quercetin significantly inhibited the differentiation of Lin−CD117+ hematopoietic stem cells into NK cells. Furthermore, quercetin promoted the proportion and maturation of NK cells by binding to the MYH9 protein.

Conclusions

In summary, our findings suggest that quercetin promotes the proportion and maturation of NK cells by binding to the MYH9 protein, thereby improving cognitive performance in middle-aged mice.

Graphical abstract
Sirtfood intake in relation to the 10-year risk of major adverse cardiovascular events: a population-based cohort study
Authors: Golzarand Mahdieh , Estaki Saghar , Mirmiran Parvin , Azizi Fereidoun
Published: May 2024

Background

Sirtuins have an important role in the regulation of metabolic and biological processess. Thus, we hypothesized that foods that could activate sirtuins, known as “sirtfood”, may improve health status. So, this study was aimed at investigating the association between the amount of sirtfood intake and the risk of major adverse cardiovascular events (MACE).

Methods

In this cohort study, 2918 adults who had no history of MACE at the start of the study (2006–2008) participated and were followed up on until 2018. The amount of sirtfoods intake (servings per week) was computed using a validated food frequency questionnaire. Each patient’s medical records were evaluated to detect MACE. The Cox proportional hazards model was applied to assess the association between the amount of sirtfood intake and the risk of MACE.

Results

The median duration of the study was 10.6 years. The hazard ratio (HR) for the risk of MACE was 0.70 for the second (95% CI: 0.50, 0.98) and 0.60 (95% CI: 0.42, 0.86) for the third tertile of sirtfoods intake compared with the first tertile. This association was nonlinear, and sirtfoods consumption of more than five servings per week did not result in a lower risk of MACE. In addition, there was a significant interaction between age (P-interaction < 0.001) and sirtfoods intake in relation to MACE occurrence. When assessing sirtfood components, compared with the lowest intake, the highest amount of soy (HR: 0.74, 95% CI: 0.56, 0.99) and parsley (HR: 0.62, 95% CI: 0.47, 0.83) intake was related to a lower risk of MACE.

Conclusion

Our results indicated an inverse association between a higher amount of sirtfood intake and a lower risk of MACE incidents. This association was nonlinear, and having more than five servings of sirtfood per week did not reduce the risk of MACE any further.
Efficacy of endotracheal intubation in helicopter cabin vs. ground: a systematic review and meta-analysis
Authors: Kolaparambil Varghese Lydia Johnson , Völlering Jan-Jakob , De Robertis Edoardo , Hinkelbein Jochen , Schmitz Jan , Warnecke Tobias
Published: May 2024

Background

Pre-hospital endotracheal intubation (ETI) is a sophisticated procedure with a comparatively high failure rate. Especially, ETI in confined spaces may result in higher difficulty, longer times, and a higher failure rate. This study analyses if Helicopter Emergency Medical Services (HEMS) intubation (time-to) success are influenced by noise, light, and restricted space in comparison to ground intubation. Available literature reporting these parameters was very limited, thus the reported differences between ETI in helicopter vs. ground by confronting parameters such as time to secure airway, first pass success rate and Cormack-Lehane Score were analysed.

Methods

A systematic review and meta-analysis were conducted using PUBMED, EMBASE, Cochrane Library, and Ovid on October 15th, 2022. The database search provided 2322 studies and 6 studies met inclusion and quality criteria. The research was registered with the International Prospective Register of Systematic Reviews (CRD42022361793).

Results

A total of six studies were selected and analysed as part of the systematic review and meta-analysis. The first pass success rate of ETI was more likely to fail in the helicopter setting as compared to the ground (82,4% vs. 87,3%), but the final success rate was similar between the two settings (96,8% vs. 97,8%). The success rate of intubation in literature was reported higher in physician-staffed HEMS than in paramedic-staffed HEMS. The impact of aircraft type and location inside the vehicle on intubation success rates was inconclusive across studies. The meta-analysis revealed inconsistent results for the mean duration of intubation, with one study reporting shorter intubation times in helicopters (13,0s vs.15,5s), another reporting no significant differences (16,5s vs. 16,8s), and a third reporting longer intubation times in helicopters (16,1s vs. 15,0s).

Conclusion

Further research is needed to assess the impact of environmental factors on the quality of ETI on HEMS. While the success rate of endotracheal intubation in helicopters vs. on the ground is not significantly different, the duration and time to secure the airway, and Cormack-Lehane Score may be influenced by environmental factors. However, the limited number of studies reporting on these factors highlights the need for further research in this area.
Optimal trade-off between boosted tolerance and growth fitness during adaptive evolution of yeast to ethanol shocks
Authors: Jacobus Ana Paula , Cavassana Stella Diogo , de Oliveira Isabelle Inácio , Barreto Joneclei Alves , Rohwedder Ewerton , Frazzon Jeverson , Basso Thalita Peixoto , Basso Luiz Carlos , Gross Jeferson
Published: May 2024

Background

The selection of Saccharomyces cerevisiae strains with higher alcohol tolerance can potentially increase the industrial production of ethanol fuel. However, the design of selection protocols to obtain bioethanol yeasts with higher alcohol tolerance poses the challenge of improving industrial strains that are already robust to high ethanol levels. Furthermore, yeasts subjected to mutagenesis and selection, or laboratory evolution, often present adaptation trade-offs wherein higher stress tolerance is attained at the expense of growth and fermentation performance. Although these undesirable side effects are often associated with acute selection regimes, the utility of using harsh ethanol treatments to obtain robust ethanologenic yeasts still has not been fully investigated.

Results

We conducted an adaptive laboratory evolution by challenging four populations (P1–P4) of the Brazilian bioethanol yeast, Saccharomyces cerevisiae PE-2_H4, through 68–82 cycles of 2-h ethanol shocks (19–30% v/v) and outgrowths. Colonies isolated from the final evolved populations (P1c–P4c) were subjected to whole-genome sequencing, revealing mutations in genes enriched for the cAMP/PKA and trehalose degradation pathways. Fitness analyses of the isolated clones P1c–P3c and reverse-engineered strains demonstrated that mutations were primarily selected for cell viability under ethanol stress, at the cost of decreased growth rates in cultures with or without ethanol. Under this selection regime for stress survival, the population P4 evolved a protective snowflake phenotype resulting from BUD3 disruption. Despite marked adaptation trade-offs, the combination of reverse-engineered mutations cyr1A1474T/usv1Δ conferred 5.46% higher fitness than the parental PE-2_H4 for propagation in 8% (v/v) ethanol, with only a 1.07% fitness cost in a culture medium without alcohol. The cyr1A1474T/usv1Δ strain and evolved P1c displayed robust fermentations of sugarcane molasses using cell recycling and sulfuric acid treatments, mimicking Brazilian bioethanol production.

Conclusions

Our study combined genomic, mutational, and fitness analyses to understand the genetic underpinnings of yeast evolution to ethanol shocks. Although fitness analyses revealed that most evolved mutations impose a cost for cell propagation, combination of key mutations cyr1A1474T/usv1Δ endowed yeasts with higher tolerance for growth in the presence of ethanol. Moreover, alleles selected for acute stress survival comprising the P1c genotype conferred stress tolerance and optimal performance under conditions simulating the Brazilian industrial ethanol production.
Demonstrating antibiotic stewardship while diagnosing and treating bilateral pseudoseptic arthritis: a case report
Authors: Tarapore Rae , Lindsey Sierra , Strickland Paige , McKinstry Robert
Published: May 2024

Introduction

Although viscosupplementation is a commonly used treatment for osteoarthritis and is widely regarded as a safe treatment option, it is associated with the rare complication of pseudoseptic arthritis. Most existing case reports that cite this rare complication employed the use of early broad-spectrum antibiotics.

Case presentation

In this case report, we present a 61-year-old African American female patient who presented with bilateral knee pseudoseptic arthritis in the setting of viscosupplementation. She presented 3 days after bilateral viscosupplementation injections with bilateral knee swelling, discomfort, and pain with micromotion. Her white blood cell count (WBC) was 12.83 (4.5–11 normal), her C-reactive protein (CRP) level was 159 mg/L (0–10 normal), and her erythrocyte sedimentation rate (ESR) was 79 mm/hour (0–40 normal). Her left knee aspirate yielded 38,580 WBC with a negative gram stain and negative cultures. Her right knee aspirate yielded 29,670 WBC with a negative gram stain and negative cultures. Through the utilization of careful clinical monitoring, ice therapy, and non-steroidal inflammatory medication, we were able to successfully treat this patient while maintaining proper antibiotic stewardship.

Conclusion

Pseudoseptic arthritis in the setting of viscosupplementation can be adequately treated and monitored without the use of antibiotics.
CIME4R: Exploring iterative, AI-guided chemical reaction optimization campaigns in their parameter space
Authors: Humer Christina , Nicholls Rachel , Heberle Henry , Heckmann Moritz , Pühringer Michael , Wolf Thomas , Lübbesmeyer Maximilian , Heinrich Julian , Hillenbrand Julius , Volpin Giulio , Streit Marc
Published: May 2024

Chemical reaction optimization (RO) is an iterative process that results in large, high-dimensional datasets. Current tools allow for only limited analysis and understanding of parameter spaces, making it hard for scientists to review or follow changes throughout the process. With the recent emergence of using artificial intelligence (AI) models to aid RO, another level of complexity has been added. Helping to assess the quality of a model’s prediction and understand its decision is critical to supporting human-AI collaboration and trust calibration. To address this, we propose CIME4R—an open-source interactive web application for analyzing RO data and AI predictions. CIME4R supports users in (
i
) comprehending a reaction parameter space, (
ii
) investigating how an RO process developed over iterations, (
iii
) identifying critical factors of a reaction, and (
iv
) understanding model predictions. This facilitates making informed decisions during the RO process and helps users to review a completed RO process, especially in AI-guided RO. CIME4R aids decision-making through the interaction between humans and AI by combining the strengths of expert experience and high computational precision. We developed and tested CIME4R with domain experts and verified its usefulness in three case studies. Using CIME4R the experts were able to produce valuable insights from past RO campaigns and to make informed decisions on which experiments to perform next. We believe that CIME4R is the beginning of an open-source community project with the potential to improve the workflow of scientists working in the reaction optimization domain.

Scientific contribution

To the best of our knowledge, CIME4R is the first open-source interactive web application tailored to the peculiar analysis requirements of reaction optimization (RO) campaigns. Due to the growing use of AI in RO, we developed CIME4R with a special focus on facilitating human-AI collaboration and understanding of AI models. We developed and evaluated CIME4R in collaboration with domain experts to verify its practical usefulness.
Retraction Note: Research on privacy and secure storage protection of personalized medical data based on hybrid encryption
Authors: Lv Jialu
Published: May 2024

This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1186/s13635-023-00150-3.
Explicit constants in the nonuniform local limit theorem for Poisson binomial random variables
Authors: Auld Graeme , Neammanee Kritsana
Published: May 2024

In a recent paper the authors proved a nonuniform local limit theorem concerning normal approximation of the point probabilities $P(S=k)$
P
(
S
=
k
)
when $S=\sum_{i=1}^{n}X_{i}$
S
=

∑

i
=
1

n

X
i

and $X_{1},X_{2},\ldots ,X_{n}$

X
1

,

X
2

,
…
,

X
n

are independent Bernoulli random variables that may have different success probabilities. However, their main result contained an undetermined constant, somewhat limiting its applicability. In this paper we give a nonuniform bound in the same setting but with explicit constants. Our proof uses Stein’s method and, in particular, the K-function and concentration inequality approaches. We also prove a new uniform local limit theorem for Poisson binomial random variables that is used to help simplify the proof in the nonuniform case.
Preeclamptic heart failure — perioperative concerns and management: a narrative review
Authors: Bandyopadhyay Anjishnujit , Puri Sunaakshi , Samra Tanvir , Ashok Vighnesh
Published: May 2024

Preeclampsia is an important cause of heart failure during pregnancy and the postpartum period. The aim of this review is to elucidate the pathophysiology and clinical features of preeclamptic heart failure and describe the medical and anesthetic management of these high-risk parturients. This article reviews the current evidence base regarding preeclamptic heart failure and its pathophysiology, types, and clinical features. We also describe the medical and anesthetic management of these patients during the peripartum period. Heart failure due to preeclampsia can present as either systolic or diastolic dysfunction. The management strategies of systolic heart failure include dietary salt restriction, diuresis, and cautious use of beta-blockers and vasodilators. Diuretics are the mainstay in the treatment of diastolic heart failure. In the absence of obstetric indications, vaginal delivery is the safest mode of delivery in these high-risk patients, and the use of an early labor epidural for analgesia is recommended. These patients would require increased invasive monitoring during labor and vaginal delivery. Neuraxial and general anesthesia have been used successfully for cesarean section in these patients but require crucial modifications of the standard technique. Uterotonic drugs have significant cardiovascular and pulmonary effects, and a clear understanding of these is essential during the management of these patients. Preeclamptics with heart failure require individualized peripartum care, as cardiac decompensation is an important risk factor for maternal and neonatal morbidity and mortality. These high-risk parturients benefit from timely multidisciplinary team inputs and collaborated management.
Functional characterization of helminth-associated Clostridiales reveals covariates of Treg differentiation
Authors: Sargsian Shushan , Mondragón-Palomino Octavio , Lejeune Alannah , Ercelen Defne , Jin Wen-Bing , Varghese Alan , Lim Yvonne A. L. , Guo Chun-Jun , Loke P’ng , Cadwell Ken
Published: May 2024

Background

Parasitic helminths influence the composition of the gut microbiome. However, the microbiomes of individuals living in helminth-endemic regions are understudied. The Orang Asli, an indigenous population in Malaysia with high burdens of the helminth Trichuris trichiura, display microbiotas enriched in Clostridiales, an order of spore-forming obligate anaerobes with immunogenic properties. We previously isolated novel Clostridiales that were enriched in these individuals and found that a subset promoted the Trichuris life cycle. In this study, we aimed to further characterize the functional properties of these bacteria.

Results

Clostridiales isolates were profiled for their ability to perform 57 enzymatic reactions and produce short-chain fatty acids (SCFAs) and hydrogen sulfide, revealing that these bacteria were capable of a range of activities associated with metabolism and host response. Consistent with this finding, monocolonization of mice with individual isolates identified bacteria that were potent inducers of regulatory T-cell (Treg) differentiation in the colon. Comparisons between variables revealed by these studies identified enzymatic properties correlated with Treg induction and Trichuris egg hatching.

Conclusion

We identified Clostridiales species that are sufficient to induce high levels of Tregs. We also identified a set of metabolic activities linked with Treg differentiation and Trichuris egg hatching mediated by these newly isolated bacteria. Altogether, this study provides functional insights into the microbiotas of individuals residing in a helminth-endemic region.
Seasonal variation of Salmonella enterica prevalence in milk and cottage cheese along the dairy value chain in three regions of Ethiopia
Authors: Admasu Henok Nahusenay , Bedassa Abdi , Tessema Tesfaye Sisay , Kovac Jasna , Vipham Jessie L. , Woldegiorgis Ashagrie Zewdu
Published: May 2024

Seasonal fluctuations influence foodborne illness transmission and affect patterns of microbial contamination of food. Previous investigations on the seasonality of Salmonella enterica prevalence in dairy products in Ethiopia have been minimal. However, such data are needed to inform strategic development of effective interventions to improve food safety, as seasonal differences may affect intervention strategies. This study was conducted to identify differences in the prevalence of Salmonella in milk and cheese samples between wet and dry seasons. A longitudinal study design was utilized with a random sampling occurring during both dry and wet seasons. A total of 448 milk and cottage cheese samples were collected from Oromia, Sidama, and Amhara regions. Samples were tested for Salmonella using the ISO 6579-1: 2008 method, followed by PCR confirmation. A chi-square test was conducted to assess the significance of differences in the prevalence of Salmonella in the samples between the two seasons. Results from this study showed a higher prevalence of Salmonella in all sample types during the dry season (P < 0.05). Moreover, when comparing raw milk, pasteurized milk, and cottage cheese samples, a significant difference was observed in Salmonella prevalence from raw milk samples (27.08%) collected in the Oromia region. Additionally, data showed a significantly higher prevalence of Salmonella in samples collected from raw milk producers (29.17%) during the wet season (P < 0.05). This study indicates that in order to enhance the safety of dairy products in Ethiopia, comprehensive, long-term awareness building on hygienic milk production and handling that consider seasonal influence is warranted.
New source model for the 1771 Meiwa tsunami along the southern Ryukyu Trench inferred from high-resolution tsunami calculation
Authors: Nakata Koki , Goto Kazuhisa , Yanagisawa Hideaki
Published: May 2024

The 1771 Meiwa tsunami which struck the southern Ryukyu Islands (Sakishima Islands) had greater than 22 m run-up height, leaving about 12,000 casualties in its wake. At many places, the tsunami inundation or lack of inundation is well recorded in historical documents. Several tsunami source models have been proposed for this event using historical records as constraints of tsunami calculations. Nevertheless, the source model remains under discussion. This study re-evaluated the tsunami wave source model of the 1771 Meiwa tsunami using high-resolution (10 m mesh) bathymetric and topographical data for tsunami calculation, the latest historical record dataset, and seismological knowledge. Results demonstrated that a tsunami earthquake along the southern Ryukyu Trench was the likely cause of the 1771 event. However, it is noteworthy that assumption of a large slip with 30 m is necessary for a shallow and narrow region (fault depth = 5 km, fault width = 30 km, Mw = 8.49) of the plate boundary in the Ryukyu Trench, which is far larger than previously thought. This requirement of very large initial water level change at the source might involve not only the fault rupture along the plate boundary but also deformation by splay faults, inelastic deformation of unconsolidated sediments near the trench axis, and/or giant submarine landslides. Results also show that the effects of fault parameters on the run-up were quite different depending on the offshore coral reef width. This phenomenon strongly constrained the fault width to 30 km. Our tsunami ray tracing analysis further revealed the effects of bathymetry on tsunami propagation. It is noteworthy that meter-long huge tsunami boulders tend to be distributed along the specific coasts at which the tsunami was concentrated by bathymetric effects. This finding suggests that past tsunamis, including the 1771 event, might have affected the specific coral reefs on Sakishima Islands repeatedly, which is crucially important for understanding the heterogeneous distribution of tsunami boulders. This feature might also be useful to elucidate the effects of large tsunamis on the corals and reefs because a direct comparison of coral reefs that are damaged and not damaged by tsunami waves is testable in narrow areas in the case of the Sakishima Islands.
Citrate-based dietary alkali supplements available in Germany: an overview
Authors: Storz Maximilian Andreas , Ronco Alvaro Luis
Published: May 2024

Background

Fruits and vegetables are abundant in alkali precursors and effectively reduce the Potential Renal Acid Load (PRAL) from diet. Oral alkali supplements are supposed to exert comparable alkalizing effects on the human body, and have been shown to beneficially affect bone and kidney health. A comparative analysis of the available dietary alkali supplements in Germany was performed, contrasting their potential PRAL-lowering potential.

Methods

We reviewed the currently available dietary citrate-based alkali supplements sold in Germany with a special focus on their mineral content, their PRAL-lowering potential and other characteristics inherent to each product. Supplements containing either potassium-, calcium- or magnesium citrate or any combination of these organic salts were reviewed. The total alkali load (TAL) was calculated based on the recommended daily dosage (RDD).

Results

Sixteen supplements with a mean alkali powder content of 220.69 ± 111.02 g were identified. The mean magnesium content per RDD was 239.93 ± 109.16 mg. The mean potassium and median calcium content were 550 ± 325.58 mg and 280 (240) mg, respectively. Median TAL was 1220 (328.75) mg. The PRAL-lowering potential from a single RDD ranged from − 51.65 mEq to -8.32 mEq. Substantial price differences were found, and the mean price of the examined supplements was 16.67 ± 5.77 Euros. The median price for a 1 mEq PRAL-reduction was 3.01 (3.14) cents, and ranged from 0.77 cents to 10.82 cents.

Conclusions

Noticeable differences between the identified alkali supplements were encountered, warranting an individual and context-specific approach in daily clinical practice.
Association between tenofovir plasma trough concentrations in the early stage of administration and discontinuation of up to five years tenofovir disoproxil fumarate due to renal function-related adverse events in Japanese HIV-1 infected patients
Authors: Yagura Hiroki , Watanabe Dai , Nakauchi Takao , Kushida Hiroyuki , Hirota Kazuyuki , Nishida Yasuharu , Yoshino Munehiro , Uehira Tomoko , Shirasaka Takuma
Published: May 2024

Background

The relationship between plasma tenofovir (TFV) concentration at the beginning of tenofovir disoproxil fumarate (TDF) administration and the development of renal dysfunction during long-term administration of TDF has not been demonstrated yet. The objective of the present study was to determine whether plasma TFV trough concentrations during early TDF administration could serve as an indicator of renal dysfunction when TDF is administered for long periods.

Methods

We included 149 HIV-1 infected Japanese patients who were prescribed TDF. We investigated the relationship between plasma TFV trough concentrations and the rate of discontinuation due to the development of renal dysfunction for up to five years after the start of TDF administration. We also examined how the decrease in renal function over time due to TDF administration was related to factors associated with high TFV levels and plasma TFV trough concentrations.

Results

The median TFV trough concentration in the TDF discontinuation group was 88 ng/mL, which was significantly higher (p = 0.0041), than that in the continuation group (72 ng/mL). Further, using an ROC curve, the cut-off value for TFV trough concentration at which TDF discontinuation was significantly high was found to be 98 ng/mL. Logistic multivariate analysis of factors associated with discontinuation of TDF due to renal function-related adverse events showed that being ≥ 50 years old (OR = 2.96; 95% CI, 1.01–8.64), having eGFR < 80 mL/min/1.73m2 at the start of TDF administration (OR = 5.51; 95% CI, 1.83–17.5), and TFV trough concentration ≥ 98 ng/mL (OR = 2.96; 95% CI, 1.16–7.60) were independent factors.

Conclusions

The results suggested that the importance of measuring TFV concentrations to evaluate the risk of developing renal function-related adverse events during long-term TDF administration.
Plyometric Jump Training Effects on Maximal Strength in Soccer Players: A Systematic Review with Meta-analysis of Randomized-Controlled Studies
Authors: Sanchez-Sanchez Javier , Rodriguez-Fernandez Alejandro , Granacher Urs , Afonso José , Ramirez-Campillo Rodrigo
Published: May 2024

Background

Maximal strength may contribute to soccer players’ performance. Several resistance training modalities offer the potential to improve maximal strength. During recent years, a large number of plyometric jump training (PJT) studies showed evidence for maximal strength improvements in soccer players. However, a comprehensive summary of the available data is lacking.

Objective

To examine the effects of PJT compared with active, passive or intervention controls on the maximal strength of soccer players, irrespective of age, sex or competitive level.

Methods

To perform a systematic review with meta-analysis following PRISMA 2020. Three electronic databases (PubMed, Web of Science, and SCOPUS) were systematically searched. Studies published from inception until March 2023 were included. A PICOS approach was used to rate studies for eligibility. The PEDro scale was used to assess risk of bias. Meta-analyses were performed using the DerSimonian and Laird random-effects model if ≥ 3 studies were available. Moderator and sensitivity analyses were performed, and meta-regression was conducted when ≥ 10 studies were available for a given comparison. We rated the certainty of evidence using GRADE.

Results

The search identified 13,029 documents, and from these 30 studies were eligible for the systematic review, and 27 for the meta-analyses. Overall, 1,274 soccer players aged 10.7–25.0 years participated in the included studies. Only one study recruited females. The PJT interventions lasted between 5 and 40 weeks (median = 8 weeks), with 1–3 weekly sessions. Compared to controls, PJT improved maximal dynamic strength (18 studies, 632 participants [7 females], aged 12.7–24.5 y; effect size [ES] = 0.43, 95% confidence interval [CI] = 0.08–0.78, p = 0.017, impact of statistical heterogeneity [I2] = 77.9%), isometric strength (7 studies; 245 participants, males, aged 11.1–22.5 y; ES = 0.58, 95% CI = 0.28–0.87, p < 0.001, I2 = 17.7%), and isokinetic peak torque (5 studies; 183 participants, males, aged 12.6–25.0 y; ES = 0.51, 95% CI = 0.22–0.80, p = 0.001, I2 = 0.0%). The PJT-induced maximal dynamic strength changes were independent of participants’ age (median = 18.0 y), weeks of intervention (median = 8 weeks), and total number of training sessions (median = 16 sessions). The certainty of evidence was considered low to very low for the main analyses.

Conclusions

Interventions involving PJT are more effective to improve maximal strength in soccer players compared to control conditions involving traditional sport-specific training.

Trial Registration The trial registration protocol was published on the Open Science Framework (OSF) platform in December 2022, with the following links to the project (https://osf.io/rpxjk) and to the registration (https://osf.io/3ruyj).
Frailty concept in Africa neurosurgical practice: a prospective review
Authors: Alare Kehinde , Jagunmolu Habiblah , Adewuyi Esther , Oluwafemi Taiwo , Olaniyi Peter , Amuzat Precious
Published: May 2024

Neurosurgical management of older patients is very complex and delicate; efforts are being made in identifying groups of older patients at risk of poor surgical outcomes before the surgery, making frailty an important concept in risk Identification and postoperative outcome assessment of older patients in the ever-changing neurosurgical world. It's of great importance for the concept of frailty to be adopted in Africa's neurosurgical practice in other to optimize the outcomes and improve the efficiency of neurosurgery in the older patients whose population is expanding, forming a great quota of people using neurosurgical services in Africa. This article looks into the importance and challenges of frailty studies in African neurosurgical practice which mainly are the longer length of time in carrying out such studies; reduces manpower, lack of availability of specialized equipment in standardizing the assessment, and others; makes recommendations on ways to ease its adoption such as funding frailty research and creation of population-specific frailty assessment tools among others.
In vitro comparison of the leakage of carbon dioxide and iodine contrast media in a bleeding model
Authors: Kitamura Ryoichi , Yoshida Kazuhiro , Maruhashi Takaaki , Tamura Satoshi , Kurihara Yutaro , Suzuki Koyo , Asari Yasushi
Published: May 2024

Background

We aimed to compare the hydrodynamic values of carbon dioxide (CO2) and iodine contrast media for bleeding detection using an in vitro model.

Materials and methods

We created a bleeding model with large and small wounds in simulated blood vessels. We connected a syringe to the bleeding model and the blood pressure transducer, filling the circuit with CO2 and iodine contrast media. The syringe’s piston was pressed, and the flow rate and intravascular pressure of the CO2 and iodine contrast media leaking from the bleeding model were measured. We compared each leaked contrast medium’s volume, sphere-equivalent diameter, and sphere-equivalent area. These values were analyzed to compare the visibility of the leakage objectively.

Results

At a constant flow rate, the intravascular pressure required for the model to leak was lower for the CO2 than that for the iodine contrast medium. The CO2 contrast medium leakage volume, equivalent circle diameter, and equivalent circle area were greater than those of the iodine one. These values indicate higher CO2 visibility during fluoroscopy.

Conclusions

In the bleeding model, a CO2 contrast medium may be more prone to leakage than the iodine one in large and small wounds. Regarding visibility, a CO2 contrast medium may be more likely to detect leakage than an iodine one.
Genetic-driven biomarkers for liver fibrosis through bioinformatic approach
Authors: Paulina Ariza Julia , Mutiara Y. Vitriyanna , Irham Lalu Muhammad , Darmawi Darmawi , Qiyaam Nurul , Firdayani Firdayani , Pitaloka Dian Ayu Eka , Arfianti Arfianti , Adikusuma Wirawan
Published: May 2024

Background

Liver fibrosis is a widespread chronic liver ailment linked to substantial mortality and limited therapeutic options. An in-depth comprehension of the genetic underpinnings of liver fibrogenesis is crucial for the development of effective management and treatment approaches.

Results

Using bioinformatics tools and the DisGeNET database, we pinpointed 105 genes significantly linked to liver fibrosis. Subsequently, we conducted functional assessments, incorporating gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and the STRING database, to construct protein–protein interaction networks (PPI) for these 105 liver fibrosis-associated genes. These analyses were executed via the WebGestalt 2019 online platform. We employed Cytoscape plugins, MCODE, and CytoHubba, to identify potential biomarker genes from these functional networks. Noteworthy hub genes encompassed TGF-β1, MMP2, CTNNB1, FGF2, IL6, LOX, CTGF, SMAD3, ALB, and VEGFA. TGF-β1 and MMP-2 exhibited substantial promise as liver fibrosis biomarkers, as denoted by their high systemic scores determined through the MCC algorithm in the CytoHubba methodology.

Conclusions

In summary, this study presents a robust genetic biomarker strategy that may prove invaluable in the identification of potential liver fibrosis biomarkers.
Decoding bovine coronavirus immune targets: an epitope informatics approach
Authors: Rani Swati , Khatoon Mehnaj , Hiremath Jagadish , Suresh Kuralayanapalya Puttahonnappa , Anandakumar Jayashree , Barman Nagendra Nath , Manjunath Sheethal , S Yamini Sri , Patil Sharanagouda S.
Published: May 2024

Bovine coronavirus (BCoV) poses a significant threat to the global cattle industry, causing both respiratory and gastrointestinal infections in cattle populations. This necessitates the development of efficacious vaccines. While several inactivated and live BCoV vaccines exist, they are predominantly limited to calves. The immunization of adult cattle is imperative for BCoV infection control, as it curtails viral transmission to calves and ameliorates the impact of enteric and respiratory ailments across all age groups within the herd. This study presents an in silico methodology for devising a multiepitope vaccine targeting BCoV. The spike glycoprotein (S) and nucleocapsid (N) proteins, which are integral elements of the BCoV structure, play pivotal roles in the viral infection cycle and immune response. We constructed a remarkably effective multiepitope vaccine candidate specifically designed to combat the BCoV population. Using immunoinformatics technology, B-cell and T-cell epitopes were predicted and linked together using linkers and adjuvants to efficiently trigger both cellular and humoral immune responses in cattle. The in silico construct was characterized, and assessment of its physicochemical properties revealed the formation of a stable vaccine construct. After 3D modeling of the vaccine construct, molecular docking revealed a stable interaction with the bovine receptor bTLR4. Moreover, the viability of the vaccine’s high expression and simple purification was demonstrated by codon optimization and in silico cloning expression into the pET28a (+) vector. By applying immunoinformatics approaches, researchers aim to better understand the immune response to bovine coronavirus, discover potential targets for intervention, and facilitate the development of diagnostic tools and vaccines to mitigate the impact of this virus on cattle health and the livestock industry. We anticipate that the design will be useful as a preventive treatment for BCoV sickness in cattle, opening the door for further laboratory studies.
Toward innovative veterinary nanoparticle vaccines
Authors: Sun Meiqi , Pratama Aldryan Cristianto , Qiu He , Liu Zehui , He Fang
Published: May 2024

Nanoparticles are significant for veterinary vaccine development because they are safer and more effective than conventional formulations. One promising area of research involves self-assembled protein nanoparticles (SAPNs), which have shown potential for enhancing antigen-presenting cell uptake, B-cell activation, and lymph node trafficking. Numerous nanovaccines have been utilized in veterinary medicine, including natural self-assembled protein nanoparticles, rationally designed self-assembled protein nanoparticles, animal virus-derived nanoparticles, bacteriophage-derived nanoparticles, and plant-derived nanoparticles, which will be discussed in this review. SAPN vaccines can produce robust cellular and humoral immune responses and have been shown to protect against various animal infectious diseases. This article attempts to summarize these diverse nanovaccine types and their recent research progress in the field of veterinary medicine. Furthermore, this paper highlights their disadvantages and methods for improving their immunogenicity.
How Do Students Critically Evaluate Outdated Language That Relates to Gender in Biology?
Authors: Dunk Ryan D. P. , Malmquist Sarah J. , Prescott Kristina K. , Ewell Sharday N. , Henning Jeremiah A. , Ballen Cissy J.
Published: May 2024

Students were not able to recognize the inherent gender implied by terms such as “woman” though they frequently corrected text about an infant’s gender. This language is common in textbooks, and this paper shows that more work is needed to rid the biology curriculum of implied equality between an individual’s gender and their sex characteristics.
AI, International Relations & Religion
Authors: Chatzivasileiou Dimitra , Psomiadi Anastasia , Efthymiou-Egleton Theoharris William , Kassar Laura
Published: May 2024

This research envisions a future where humans and machines collaboratively enhance decision-making capabilities, fostering harmonious coexistence. Addressing concerns about the potential threat of artificial intelligence (AI) to humanity, the focus shifts to the benevolence of AI entities shaped by human influence. The prospect of AI functioning at a level where authority is wielded by an inaccessible and infallible entity lies in its role as an independent arbiter. This entails the capability to identify cultural barriers and navigate existing political constraints deliberately. Consequently, there is potential for discovering common political ground through algorithmic processes, leading to the resolution of longstanding political issues between states. However, uncertainties persist – perhaps these aspirations may not materialize as expected. The study explores AI's role in international relations and religion, particularly Christianity, emphasizing its potential as an independent arbiter capable of recognizing cultural barriers and navigating political constraints. This research explores the intersection of cultural sensitivity and AI in diplomacy, discussing ethical considerations and benefits. The impact of AI on conflict resolution and peacebuilding is examined, stressing the need for collaborative efforts to establish robust AI standards. Challenges to religious authority, ethical considerations in AI development, and AI's influence on humanitarian aid and religious values are also explored. The research concludes by highlighting the imperative to address algorithmic bias for inclusivity and equitable representation in the digital age.
Identification of latent contraceptive ideational profiles among urban women in Senegal: Transitions and implications for family planning programs
Authors: Mangone Emily , Speizer Ilene , O'Shea Nisha , Hassmiller Lich Kristen
Published: May 2024

Background Latent ideational segmentation is an important technique that can enhance family planning (FP) communication campaigns by providing insight into prototypical “profiles” of women among heterogenous populations based on shared ideational characteristics that underpin contraceptive decision-making. This can improve the development of responsive, tailored content and help programs connect with intended audiences. In Senegal, 24% of married women who want to avoid pregnancy are not using modern contraceptive methods and in 2020, the Government of Senegal fell short of reaching its goal of increasing the modern contraceptive prevalence to 45%. Social, cultural, and cognitive factors are probable deterrents to contraceptive use. The objective of this study was to identify and interpret meaningful contraceptive ideational profiles (CIPs) among urban Senegalese women and examine how and why CIP structure, interpretation, and membership changed over time. Methods Using longitudinal data from 4,047 urban, in-union Senegalese women of reproductive age in 2011 and 2015, we applied latent transition analysis to identify and interpret prototypical profiles of women based on their contraceptive awareness, beliefs, self-efficacy, partner FP acceptance, partner communication, and community support. Results We identified four longitudinal CIPs and labeled them “CIP1: Lowest efficacy and FP awareness, highest misconceptions, unsupported,” “CIP2: Low efficacy and FP awareness, rejects misconceptions, unsupported,” “CIP3: Moderate efficacy, high FP awareness, high misconceptions, moderate support,” and “CIP4: Highest efficacy and FP awareness, fewest misconceptions, most supported.” At endline, more women were in higher-order CIPs compared to baseline. Exposure to FP communication via TV, radio, religious leaders, and health workers was associated with lower odds of membership in lower-order CIPs at endline, as was exposure to messages about FP and birth spacing. Conclusions This study demonstrated the potential of latent CIP methodologies to enhance current social and behavior change approaches by identifying and responding to unique and complex ideational attributes.
Exploring the potential of blockchain technology for citizen engagement in smart governance
Authors: Testi Niccolò , Marconi Rebecca , Pasher Edna
Published: May 2024

This review article explores the potential of blockchain technology (BCT) as a key enabler for fostering transparency, trust, and citizen engagement in smart governance within smart cities. By examining the benefits of BCT in various aspects of smart city systems, such as data security and privacy, the paper highlights the advantages of implementing consortium blockchain architecture and the Proof of Authority (PoA) consensus algorithm and discusses the challenges of scalability and security concerns. Based on the literature showed in this article, we believe that the use of BCT in smart governance could significantly enhance citizen participation and help manage and deliver public services, paving the way for more effective decision-making processes and improved quality of life for citizens.
Discapacidad e interés público: experiencias no tradicionales de litigio estratégico en la clínica jurídica en discapacidad y derechos humanos de la Pontificia Universidad Católica del Perú
Authors: Bregaglio Renata
Published: May 2024

Las clínicas jurídicas, a través de acciones de litigio estratégico, son un espacio clave para la enseñanza del derecho y para desmontar estructuras de opresión. El entendimiento mayoritario del litigio estratégico ha sido la tramitación de casos ante las altas cortes. Sin embargo, existen otras alternativas que pueden ser igualmente satisfactorias y que permiten transversalizar los enfoques que trabajan las clínicas en otras ramas del derecho. En el presente capítulo se presenta el trabajo de litigio estratégico de la Clínica Jurídica en Discapacidad y Derechos Humanos de la Facultad de Derecho de la Pontificia Universidad Católica del Perú (PUCP) en instancias administrativas, y que se salen del patrón tradicional de una decisión de interés público emitida por una alta corte.
El cuándo, el cómo y el porqué de la Clínica Jurídica per la Justícia Social de la Universitat de València: 15 años de experiencia
Authors: Fernández Artiach Pilar , Gascón Cuenca Andrés
Published: May 2024

El movimiento jurídico clínico está experimentado un auge muy importante en la realidad universitaria española. Cada vez más universidades cuentan con este tipo de proyectos formativos que intentan enseñar el derecho desde la óptica de la justicia social y los derechos humanos. En esta contribución presentamos la experiencia de la Clínica Jurídica per la Justícia Social de la Facultat de Dret de la Universitat de València.