bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional analysis of CYP71AV1 reveals the evolutionary landscape of artemisinin biosynthesis
Authors: Chen Fang-Yan , Mu Qiu-Yan , Xu Bing-Yi , Lei Yu-Chen , Liu Hui-Ying , Fang Xin
Published: March 2024

Artemisinin biosynthesis, unique to Artemisia annua, is suggested to have evolved from the ancestral costunolide biosynthetic pathway commonly found in the Asteraceae family. However, the evolutionary landscape of this process is not fully understood. The first oxidase in artemisinin biosynthesis, CYP71AV1, also known as amorpha-4,11-diene oxidase (AMO), has specialized from ancestral germacrene A oxidases (GAOs). Unlike GAO, which exhibits catalytic promiscuity toward amorpha-4,11-diene, the natural substrate of AMO, AMO has lost its ancestral activity on germacrene A. Previous studies have suggested that the loss of the GAO copy in A. annua is responsible for the abolishment of the costunolide pathway. In the genome of A. annua, there are two copies of AMO, each of which has been reported to be responsible for the different product profiles of high- and low-artemisinin production chemotypes. Through analysis of their tissue-specific expression and comparison of their sequences with those of other GAOs, it was discovered that one copy of AMO (AMOHAP) exhibits a different transcript compared to the reported artemisinin biosynthetic genes and shows more sequence similarity to other GAOs in the catalytic regions. Furthermore, in a subsequent in vitro enzymatic assay, the recombinant protein of AMOHAP unequivocally demonstrated GAO activity. This result clearly indicates that AMOHAP is a GAO rather than an AMO and that its promiscuous activity on amorpha-4,11-diene has led to its misidentification as an AMO in previous studies. In addition, the divergent expression pattern of AMOHAP compared to that of the upstream germacrene A synthase may have contributed to the abolishment of costunolide biosynthesis in A. annua. Our findings reveal a complex evolutionary landscape in which the emergence of a new metabolic pathway replaces an ancestral one.
Epidemiology of human papillomavirus infection in women from Xiamen, China, 2013 to 2023
Authors: Yao Xingmei , Li Qing , Chen Yu , Du Zhuowen , Huang Yanru , Zhou Yixi , Zhang Jian , Wang Wenbo , Zhang Lutan , Xie Jieqiong , Xu Chao , Ge Yunsheng , Zhou Yulin
Published: March 2024

Background

Cervical cancer is primarily caused by HPV infection. The epidemiology of HPV infection in specific areas is of great meaning of guide cervical cancer screening and formulating HPV vaccination strategies. Here, we evaluated the epidemiological characteristics of HPV infection in Xiamen population.

Methods

In total, 159,049 cervical exfoliated cell samples collected from female outpatients in Women and Children’s Hospital, School of Medicine, Xiamen between January 2013 and July 2023 were analyzed. HPV DNA detection was performed using HPV genotyping kits (Hybribio Limited Corp, China). An analysis was conducted on the prevalence of HPV infection, taking into account factors such as age, year, and multiple patterns of HPV infection. The differences in prevalence among age groups and years were compared using χ2 test.

Results

The overall prevalence of any 21 HPV genotypes was 18.4%, of which the high-risk HPV (HR-HPV) positive rate was 14.6%. The age-specific prevalence of HPV infection showed a bimodal distribution, with two distinct peaks, one at <25 years (31.2%) and the other at 60–64 years (32.9%). There was a downward trend in the prevalence of HPV infection over time, decreasing from 26.2% in 2013 to 14.5% in 2021, and then increasing to 19.0% in 2023. The five most prevent HR-HPV genotypes were HPV52 (4.0%), 58 (2.6%), 16 (2.5%), 51 (1.8%), and 39 (1.7%). Among the positive cases, 76.7% were detected with only one genotype and 23.3% with multiple genotypes. The most common co-infection was HPV52 + HPV58 (0.24%), followed by HPV16 + HPV52 (0.24%), HPV52 + HPV53 (0.21%), HPV52 + HPV81 (0.21%), HPV51 + HPV52 (0.19%), HPV16 + HPV58 (0.18%), and HPV39 + HPV52 (0.17%).

Conclusion

The study provided the largest scale information on the recent epidemiological characteristics of HPV infection in Xiamen, and even in Fujian Province, China, which would support making the prevention and control strategies for cervical cancer in the region.
Receptivity of providing firearm safety storage devices to parents along with firearms safety education
Authors: Fraser Doh Kiesha , Bishop Zhana , Gillings Trishanne , Johnson Jonathan , Boy Angela , Waris Rabbia S. , Bhatia Amina M. , Santore Matthew T. , Simon Harold K.
Published: March 2024

Background

In the United States, 33% of households with children contain firearms, however only one-third reportedly store firearms securely. It’s estimated that 31% of unintentional firearm injury deaths can be prevented with safety devices. Our objective was to distribute safe storage devices, provide safe storage education, evaluate receptivity, and assess impact of intervention at follow-up.

Method

At five independent, community safety events, parents received a safe storage device after completing a survey that assessed firearms storage methods and parental comfort with discussions regarding firearm safety. Follow-up surveys collected 4 weeks later. Data were evaluated using descriptive analysis.

Result

320 participants completed the surveys, and 288 participants were gunowners living with children. Most participants were comfortable discussing safe storage with healthcare providers and were willing to talk with friends about firearm safety. 54% reported inquiring about firearm storage in homes their children visit, 39% stored all their firearms locked-up and unloaded, 32% stored firearms/ammunition separately. 121 (37%0.8) of participants completed the follow-up survey, 84% reported using the distributed safety device and 23% had purchased additional locks for other firearms.

Conclusion

Participants were receptive to firearm safe storage education by a healthcare provider and distribution of a safe storage device. Our follow up survey results showed that pairing firearm safety education with device distribution increased overall use of safe storage devices which in turn has the potential to reduce the incidence of unintentional and intentional self-inflicted firearm injuries. Providing messaging to promote utilization of safe storage will impact a firearm safety culture change.
Our business, not the robot’s: family conversations about privacy with social robots in the home
Authors: Levinson Leigh , McKinney Jessica , Nippert-Eng Christena , Gomez Randy , Šabanović Selma
Published: March 2024

The targeted use of social robots for the family demands a better understanding of multiple stakeholders’ privacy concerns, including those of parents and children. Through a co-learning workshop which introduced families to the functions and hypothetical use of social robots in the home, we present preliminary evidence from 6 families that exhibits how parents and children have different comfort levels with robots collecting and sharing information across different use contexts. Conversations and booklet answers reveal that parents adopted their child’s decision in scenarios where they expect children to have more agency, such as in cases of homework completion or cleaning up toys, and when children proposed what their parents found to be acceptable reasoning for their decisions. Families expressed relief when they shared the same reasoning when coming to conclusive decisions, signifying an agreement of boundary management between the robot and the family. In cases where parents and children did not agree, they rejected a binary, either-or decision and opted for a third type of response, reflecting skepticism, uncertainty and/or compromise. Our work highlights the benefits of involving parents and children in child- and family-centered research, including parental abilities to provide cognitive scaffolding and personalize hypothetical scenarios for their children.
A Capsaicin-Based Phytogenic Solution Improves Performance and Thermal Tolerance of Heat-Stressed Growing Pigs
Authors: Cervantes Miguel , Sakkas Panagiotis , Soto Moisés , Gómez Alejandra Jaquelin , Camacho Reyna L. , Arce Néstor , Quilichini Nicolas , Morales Adriana
Published: March 2024

Exposure to heat stress (HS) detrimentally affects pig performance. This study explored whether a dietary phytogenic solution based on Capsicum spp. (PHY) could enhance the thermal tolerance of heat-stressed growing pigs. Forty-two individually housed pigs were randomly assigned to three treatments: thermoneutral pigs on a control diet (TN-C) and pigs subjected to HS fed the control diet either without (HS-C) or with supplemental PHY (HS-PHY). The TN-C group exhibited increased average daily gain (ADG) and feed intake (FI) compared to both HS-C (p < 0.01) and HS-PHY pigs (p < 0.05) and better feed efficiency compared to HS-C pigs only (p < 0.01). However, the HS-PHY pigs showed significantly higher FI (p < 0.01) and ADG (p < 0.05) compared to HS-C pigs. HS pigs displayed higher body temperatures (BTs) than TN pigs (p < 0.01), yet HS-PHY pigs experienced a lesser increase in BT compared to HS-C pigs (p < 0.05). Supplementation with PHY mitigated some effects of HS, increasing serum superoxide dismutase and catalase activity, reducing HSP90 expression in longissimus dorsi muscle, and elevating jejunal villus height compared to HS-C pigs (p < 0.05), reaching levels akin to TN-C pigs. Additionally, PHY supplementation resulted in lower serum urea levels than HS-C pigs (p < 0.01) and similar myosin gene expression to TN-C pigs (p > 0.1), suggesting enhanced amino acid post-absorptive utilization for lean tissue growth. In conclusion, dietary PHY supplementation partially offset the adverse effects of HS on pig performance by improving thermal tolerance.
A Review on Blood Reference Values as a Valuable Marker of Wildlife Welfare in Erinaceus europaeus
Authors: Rosa Sofia , Silvestre-Ferreira Ana C. , Queiroga Felisbina Luísa
Published: March 2024

The western-European hedgehog (Erinaceus europaeus), in expanding its range towards human habitats, faces exposure to contaminants and biological agents, potentially leading to diseases associated with hematological and biochemical changes. As bioindicators of environmental pollution and carriers of zoonotic agents, hedgehogs play a crucial role in One Health studies, emphasizing the need for a comprehensive understanding of their clinical-pathological aspects. Exploring the blood reference values in healthy animals of this species is crucial for understanding and improving their well-being, and identifying possible diseases/pathogens that may affect its conservation and/or impact human health. This review is focused on analyzing the data available in the literature for Erinaceus europaeus blood reference intervals. A comprehensive literature review of the studies published in Europe is performed, highlighting their specificities, and emphasizing the need for continuous research in this field. Our final goal is to provide a crucial tool for assessing the health status of the species, and underscoring the significance of research in this specific domain.
A Potential Nervous Necrosis Virus (NNV) Live Vaccine for Sole Obtained by Genomic Modification
Authors: Vázquez-Salgado Lucía , Souto Sandra , Olveira José G. , Bandín Isabel
Published: March 2024

Viral Encephalopathy and Retinopathy (VER) is a neurological infectious fish disease that causes vacuolization and necrosis in the central nervous system, which lead to swimming abnormalities and, generally, host death in the early stages of development. VER is caused by the Nervous Necrosis Virus (NNV), a non-enveloped virus with a bisegmented and positive-stranded (+) RNA genome. The largest segment (RNA1) codes for viral polymerase while capsid protein is encoded by RNA2. The aim of this study was to explore the potential of a reverse-engineered RGNNV/SJNNV strain that harbors mutations in both 3′NCRs (position 3073 of RNA1 and 1408 and 1412 of RNA2) as an attenuated live vaccine for sole. The attenuation of this strain was confirmed through experimental infections in sole at 22 °C. Vaccination trials were performed by bath, intramuscular, and intraperitoneal injection, at two temperatures (18 and 22 °C). Our results indicate the improved survival of vaccinated fish and delayed and poorer viral replication, as well as an overexpression of immune response genes linked to T cell markers (cd4 and cd8), to an early inflammatory response (tlr7 and tnfα), and to antiviral activity (rtp3 and mx). In conclusion, our study indicates that the attenuated strain is a good vaccine candidate as it favors sole survival upon infection with the wt strain while inducing a significant immune response.
The Influence of Cold Therapy on the Physical Working Capacity at the Electromyographic Threshold for Consecutive Exercise Sessions
Authors: Maasri Rami E. , Jarvie Jonathan R. , Karski Jacob S. , Smith Logan J. , Malek Moh H.
Published: March 2024

Background: The purpose of this study was to determine whether cold therapy after the first exercise test influences the physical working capacity at the fatigue threshold (PWCFT) during the second exercise test. We hypothesized that cold therapy would delay the onset of PWCFT for the second exercise test relative to the control visit (i.e., no cold therapy). Methods: Eight healthy college-aged men volunteered for the present study. For each of the two visits, subjects performed incremental, single-leg, knee-extensor ergometer, followed by either resting for 30 min (control visit) or having a cold pack applied for 15 min and then resting for 15 min (experimental visit). Then, the same exercise test was performed. The order of visits (control vs. experimental) was randomized for each subject. The exercise indices and PWCFT were determined for each of the two visits and statistically analyzed using two-way repeated measures analysis of variance. Results: The results indicate no significant (p > 0.05) mean differences for maximal power output, heart rate at end-exercise, and PWCFT between the control and cold therapy visits. Moreover, there were no significant (p > 0.05) mean differences between the first and second exercise workbout within each visit. Conclusions: The findings of this study suggest that cold therapy did not influence neuromuscular fatigue.
Leveraging Deep Learning for Fine-Grained Categorization of Parkinson’s Disease Progression Levels through Analysis of Vocal Acoustic Patterns
Authors: Malekroodi Hadi Sedigh , Madusanka Nuwan , Lee Byeong-il , Yi Myunggi
Published: March 2024

Speech impairments often emerge as one of the primary indicators of Parkinson’s disease (PD), albeit not readily apparent in its early stages. While previous studies focused predominantly on binary PD detection, this research explored the use of deep learning models to automatically classify sustained vowel recordings into healthy controls, mild PD, or severe PD based on motor symptom severity scores. Popular convolutional neural network (CNN) architectures, VGG and ResNet, as well as vision transformers, Swin, were fine-tuned on log mel spectrogram image representations of the segmented voice data. Furthermore, the research investigated the effects of audio segment lengths and specific vowel sounds on the performance of these models. The findings indicated that implementing longer segments yielded better performance. The models showed strong capability in distinguishing PD from healthy subjects, achieving over 95% precision. However, reliably discriminating between mild and severe PD cases remained challenging. The VGG16 achieved the best overall classification performance with 91.8% accuracy and the largest area under the ROC curve. Furthermore, focusing analysis on the vowel /u/ could further improve accuracy to 96%. Applying visualization techniques like Grad-CAM also highlighted how CNN models focused on localized spectrogram regions while transformers attended to more widespread patterns. Overall, this work showed the potential of deep learning for non-invasive screening and monitoring of PD progression from voice recordings, but larger multi-class labeled datasets are needed to further improve severity classification.
Coronal Knee Alignment and Tibial Rotation in Total Knee Arthroplasty: A Prospective Cohort Study of Patients with End-Stage Osteoarthritis
Authors: Strahovnik Andrej , Strahovnik Igor , Fokter Samo Karel
Published: March 2024

Several studies have found a relationship between the rotational anatomy of the distal femur and the overall coronal lower limb alignment in knees with osteoarthritis (OA). Less is known about the rotation of the proximal tibia, especially in the context of total knee arthroplasty (TKA), where one of the goals of the surgery is to achieve the appropriate component-to-component rotation. The aim of this study was to investigate the relationship between the coronal alignment of the lower extremity and the relative proximal tibial rotation. A prospective cohort study of patients with an end-stage OA scheduled for TKA was conducted. All patients underwent a computed tomography (CT) scan and a standing X-ray of both lower limbs. A relative femorotibial rotation was measured separately for mechanical and kinematic alignment. A statistically significant correlation was found between the tibial varus and the external tibial rotation (p < 0.001). Out of 14 knees with high tibial varus (>5°), 13 (93%) and 7 (50%) knees had >10° of femorotibial rotation for the mechanical and kinematic alignment landmarks, respectively. In order to keep the component-to-component rotation within the 10° margin, more internal rotation of the tibial component is required in knees with higher tibial varus.
APOE Gene Variation’s Impact on Cardiovascular Health: A Case-Control Study
Authors: Ismail Aya Badeea , Balcıoğlu Özlem , Özcem Barçın , Ergoren Mahmut Çerkez
Published: March 2024

Chronic venous insufficiency (CVI) is a common medical condition characterized by impaired functioning of the venous system in the lower extremities. It leads to various symptoms, including varicose veins, leg edema, and skin pigmentation. It is believed that a combination of genetic and environmental factors affect the development of CVI. The APOE gene is of particular interest in this context, as it plays a role in lipid metabolism and inflammation. The ε4 allele (rs429358) has been associated with an increased risk of Alzheimer’s disease, while the ε2 allele (rs7412) has shown a protective effect against Alzheimer’s disease but a strong association with cardiovascular inflammation. This research aimed to investigate the presence of APOE gene variants in individuals with chronic venous insufficiency disease and validate the relationship between this gene and cardiovascular diseases. The study analyzed the expression of APOE gene variants in varicose vein tissue samples from patients and a normal vein in the control group. The results indicated no significant expression of the ε4 allele in either group. However, there was a significant decrease in the expression of the ε2 allele in the patient group. Additionally, a negative correlation was observed between the two single nucleotide polymorphisms (SNPs) in vein tissue. The lower expression of the ε2 allele in patients suggests a potentially reduced risk of cardiovascular disease in these individuals. Consequently, there appears to be a weaker association between the expression of the APOE gene ε2 allele and cardiovascular diseases.
Strategic Design Approaches for Eliciting the Perception of ‘Prestige’ in Housing Consumers
Authors: Montañana Antoni , Nolé María Luisa , Llinares Carmen
Published: March 2024

In the current real estate landscape, there is a growing trend to focus on adding value to products to address the customer’s affective/emotional perspective, his/her perceptions of the ‘Prestige’ of properties being crucial for his/her final assessments of the products. This study delves into the design elements that shape perceptions of ‘Prestige’ using Kansei engineering in 235 participants who evaluated various real estate promotions through: (1) a set of 60 adjectives and (2) reason for purchase (residence or investment). A first factor analysis of the set of adjectives yielded 15 independent axes. A subsequent linear regression indicated that the ‘Prestige’ axis was among the four most important factors in/drivers of property purchase decisions. In the second phase, 62 design elements (and their respective categories) of real estate promotions were identified to establish 16 groups. A linear regression determined that Information presentation and Building were the groups of elements with the greatest impact on subjects’ perceptions of ‘Prestige’. A subsequent Univariate General Linear Model analysis identified the design elements significant for each group, such as Development type and Building shape, respectively. Finally, a Bonferroni post hoc test identified the important categories in each identified design element as Facing blocks and Stepped.
Resistance Factor Spectra for the Ultimate Limit State of the National Building Code of Canada
Authors: Tabsh Sami W. , Leblouba Moussa
Published: March 2024

Over the years, structural engineering codes and specifications in Canada and elsewhere have moved from an allowable stress design (ASD) approach to a load and resistance factor design (LRFD) philosophy. LRFD methodology takes better account of the inherent variability in both loading and resistance by providing different factors of safety for loads of distinct natures with regard to their probability of overload, frequency of occurrences and changes in point of application. The method also results in safer structures because it considers the behavior at collapse. While resistance factors for traditional construction materials based on LRFD in the National Building Code (NBC) of Canada are available, they cannot be used for non-conventional ones. This is because the resistance of such materials due to various load effects has unique bias factors (λR) and coefficients of variation (VR), which greatly impact their reliability index (β). In this study, relationships between the resistance factor ϕ and critical load effects from the NBC load combinations at ultimate limit states are developed for a wide range of resistance bias factors and coefficients of variation. The relationships are presented in the form of charts that are useful for researchers and code-writing professionals who have expertise in the various fields of structural engineering but lack proper background in reliability theory. The developed spectra showed that for the same ϕ, β increases with an increase in the live-to-dead load (L/D) ratio until it reaches 1; thereafter, the shape of the relationship will depend on the statistics of the resistance as well as on the magnitude of ϕ. For a small ϕ and VR, β will keep increasing with an increase in the L/D ratio from 1 until 3, albeit at a lesser rate. For L/D > 3, the relationship between the critical β and applied load is just about constant. This finding is also true for load combinations involving snow and wind. Application of the method is illustrated by a practical example involving the shear strength of a corrugated web steel beam.
Off-Label Yellow Fever and Hepatitis A Vaccination in Traveling Children
Authors: Muruzábal Cecilia , Vicente Lorea , Escolano Taravillo Lucía , Bravo Queipo de Llano Blanca , Calvo Cristina , García López Hortelano Milagros
Published: March 2024

There are few data on yellow fever (YF) and hepatitis A (HA) off-label vaccination. Given the rising trend of travel to endemic countries, there is a growing necessity to broaden vaccination coverage among the pediatric population. For this reason, we aim to assess the adverse effects associated with off-label vaccination, with the ultimate purpose of expanding the vaccine spectrum. We analyzed ambispectively ninety-four children under 12 months of age who received YF or HA off-label vaccines. The YF vaccine was administered to children aged 6–9 months and those allergic to eggs (with a prior negative prick test and no history of anaphylaxis), while the HA vaccine was given to children aged 6–12 months. Overall, 71 (75%) were vaccinated against YF, and 57 (60%) against HA; 34 against both. All of them fulfilled off-label vaccination criteria. No immediate adverse effects (AEs) were reported. Mild common AEs (diarrhea, fever, or malaise) were experienced by 10.8% of patients within 10 days after vaccination. The rate of AEs associated with off-label vaccination for HA and YF is low, suggesting that the vaccines could be considered safe.
Micro(nano)plastics and Their Potential Impact on Human Gut Health: A Narrative Review
Authors: Covello Carlo , Di Vincenzo Federica , Cammarota Giovanni , Pizzoferrato Marco
Published: March 2024

Microplastics and nanoplastics (MNPs) are becoming an increasingly severe global problem due to their widespread distribution and complex impact on living organisms. Apart from their environmental impact, the effects of MNPs on living organisms have also continued to attract attention. The harmful impact of MNPs has been extensively documented in marine invertebrates and larger marine vertebrates like fish. However, the research on the toxicity of these particles on mammals is still limited, and their possible effects on humans are poorly understood. Considering that MNPs are commonly found in food or food packaging, humans are primarily exposed to them through ingestion. It would be valuable to investigate the potential harmful effects of these particles on gut health. This review focuses on recent research exploring the toxicological impacts of micro- and nanoplastics on the gut, as observed in human cell lines and mammalian models. Available data from various studies indicate that the accumulation of MNPs in mammalian models and human cells may result in adverse consequences, in terms of epithelial toxicity, immune toxicity, and the disruption of the gut microbiota. The paper also discusses the current research limitations and prospects in this field, aiming to provide a scientific basis and reference for further studies on the toxic mechanisms of micro- and nanoplastics.
The Ethyl Acetate Extract of Caulerpa microphysa Promotes Collagen Homeostasis and Inhibits Inflammation in the Skin
Authors: Lu Kuo-Yun , Cheng Li-Ching , Hung Zheng-Ci , Chen Ze-Ying , Wang Chuang-Wei , Hou Hsin-Han
Published: March 2024

Inflammation and collagen-degrading enzymes’ overexpression promote collagen decomposition, which affects the structural integrity of the extracellular matrix. The polysaccharide and peptide extracts of the green alga Caulerpa microphysa (C. microphysa) have been proven to have anti-inflammatory, wound healing, and antioxidant effects in vivo and in vitro. However, the biological properties of the non-water-soluble components of C. microphysa are still unknown. In the present study, we demonstrated the higher effective anti-inflammatory functions of C. microphysa ethyl acetate (EA) extract than water extract up to 16–30% in LPS-induced HaCaT cells, including reducing the production of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α). Furthermore, the excellent collagen homeostasis effects from C. microphysa were proven by suppressing the matrix metalloproteinase-1 (MMP-1) secretion, enhancing type 1 procollagen and collagen expressions dose-dependently in WS1 cells. Moreover, using UHPLC-QTOF-MS analysis, four terpenoids, siphonaxanthin, caulerpenyne, caulerpal A, and caulerpal B, were identified and may be involved in the superior collagen homeostasis and anti-inflammatory effects of the C. microphysa EA extract.
Feeding Ecology of Reintroduced Golden Parakeets (Guaruba guarouba, Psittacidae) in a Protected Area in the Amazon Forest
Authors: Vilarta Marcelo Rodrigues , De Moraes Thaís Tamamoto , Gondim Maria Fernanda Naegeli , Lobato Crisomar , Da Costa Mônica Nazaré Rodrigues Furtado , Oliveira Rubens de Aquino , Silveira Luís Fábio
Published: March 2024

The Golden Parakeet is an endemic Brazilian flagship species that has suffered from poaching and habitat loss, leading to local extinctions in the urbanized parts of the Amazon. We reintroduced six groups of mostly captive-bred parakeets in a protected area. The birds were acclimatized for at least five months at the release site, where they were trained to recognize native foods and develop foraging skills. Subsequently, we conducted a soft release, followed by daily supplementation and monitoring. For three years following the release we recorded their diet, feeding behavior, and how they adapted to wild foraging. The reintroduced birds fed on 23 plant species, with 13 not being previously listed in past studies. The three most consumed species corresponded to 77% of their feeding records. Parakeets spent more time feeding in altered landscapes and secondary vegetation than in the preserved forest. Most of the feeding happened during the rainy season when most of their favorite plants are fruiting. The parakeets’ incorporation of new species in their diet and their transition from supplemental to natural feeding happened gradually, as we did not reduce food offerings. Parakeets that showed site fidelity were able to find native food rapidly following release, but individuals that dispersed immediately had more difficulty finding food. This study showed that captive-bred Golden Parakeets can transition to a wild diet following a gradual reintroduction process.
Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice
Authors: Dremelj Anja , Miceska Simona , Kuhar Anamarija , Nolde Natasa , Kloboves-Prevodnik Veronika
Published: March 2024

The withdrawal of the iView detection system (iV) forced many cytopathology laboratories, including ours, to substitute immunocytochemical (ICC) staining protocols for routine practice with other detection systems. Our objective was to optimize, validate, and implement ICC protocols using OptiView (OV) and EnVision FLEX (EnV) detection systems, comparing the results with those obtained using iV. Residual cytologic samples with known diagnoses were used, testing antibodies for the ten most common markers in routine cytopathology diagnostics (calretinin, Ber-EP4, MOC-31, CKAE1/AE3, CK5/6, CD68, LCA, desmin, HBME-1, and WT1). Different staining parameters were tested using OV on BenchMark ULTRA and EnV on Dako Omnis immunostainer, respectively. Optimal staining protocols were then selected and validated on 10 positive and 10 negative cases. The staining results were compared with iV protocols through evaluation of UK NEQAS and internal scores. The optimal staining protocols with OV and EnV demonstrated similar or superior results compared to the existing iV protocols, with slightly stronger intensity regarding positive cells. We have successfully established and validated optimal ICC staining protocols for commonly used markers in routine cytopathology practice. These protocols may benefit other laboratories using similar staining platforms. However, the challenge regarding standardizing ICC protocols across different cytopathology laboratories remains unresolved.
Air–Ground Collaborative Multi-Target Detection Task Assignment and Path Planning Optimization
Authors: Ma Tianxiao , Lu Ping , Deng Fangwei , Geng Keke
Published: March 2024

Collaborative exploration in environments involving multiple unmanned aerial vehicles (UAVs) and unmanned ground vehicles (UGVs) represents a crucial research direction in multi-agent systems. However, there is still a lack of research in the areas of multi-target detection task assignment and swarm path planning, both of which play a vital role in enhancing the efficiency of environment exploration and reducing energy consumption. In this paper, we propose an air–ground collaborative multi-target detection task model based on Mixed Integer Linear Programming (MILP). In order to make the model more suitable for real situations, kinematic constraints of the UAVs and UGVs, dynamic collision avoidance constraints, task allocation constraints, and obstacle avoidance constraints are added to the model. We also establish an objective function that comprehensively considers time consumption, energy consumption, and trajectory smoothness to improve the authenticity of the model and achieve a more realistic purpose. Meanwhile, a Branch-and-Bound method combined with the Improved Genetic Algorithm (IGA-B&B) is proposed to solve the objective function, and the optimal task assignment and optimal path of air–ground collaborative multi-target detection can be obtained. A simulation environment with multi-agents, multi-obstacles, and multi-task points is established. The simulation results show that the proposed IGA-B&B algorithm can reduce the computation time cost by 30% compared to the traditional Branch-and-Bound (B&B) method. In addition, an experiment is carried out in an outdoor environment, which further validates the effectiveness and feasibility of the proposed method.
A Review of Energy Overconsumption Reduction Methods in the Utilization Stage in Compressed Air Systems
Authors: Gryboś Dominik , Leszczyński Jacek S.
Published: March 2024

Pneumatic systems use the energy of compressed air to carry out manufacturing automation processes through the implementation of complex handling and motion tasks. However, these systems are energy intensive: it is estimated that pneumatic systems in manufacturing plants consume approximately 10% of all electricity consumed in the industrial sector. At the same time, the energy efficiency of the whole pneumatic system is observed to be 6–10%, due to the compression process, oversizing, and overconsumption. There are numerous solutions in the literature focusing on improving efficiency at the compression stage of utilization; however, for the utilization stage, there is a lack of systematization and grouping of these solutions. The following review will summarize current knowledge about the utilization stage and methods for improving oversizing and energy overconsumption. In addition, a method of exergy analysis for pneumatic systems will be presented, which is a very useful tool to assess the efficiency of these systems.
Empowering Active Users: A Case Study with Economic Analysis of the Electric Energy Cost Calculation Post-Net-Metering Abolition in Slovenia
Authors: Tratnik Eva , Beković Miloš
Published: March 2024

This paper addresses the issue of the abolition of annual net metering in Slovenia and compares the electric energy costs for the studied active user after the abolition. The article also provides an exploration of the role played by an aggregator, which serves as a central entity that enables individuals to participate in the electric energy market. An analysis of the case study of an active user was made, where an analysis was made of the measurements of household consumption and photovoltaic plant production for the year 2022. This article presents an economic analysis with and without net metering and an analysis of the aggregator involvement strategies. In addition, a battery energy storage system was also considered in the analysis. An important part of the article is the identification of the flexibility potential for shiftable loads, which enable an aggregator to acquire insight into the energy consumption profile and energy production profile of active users. The following indicators were used to compare the strategies: annual electric energy cost and the indicators including self-sufficiency, self-consumption, and grid dependency. The findings indicate that, even in the absence of annual net metering, the active user can lower their costs for electric energy with the help of an aggregator.
Characterization of a Fermented Beverage from Discarded Bread Flour Using Two Commercial Probiotics Starters
Authors: Sigüenza-Andrés Teresa , Mateo Javier , Rodríguez-Nogales José M. , Gómez Manuel , Caro Irma
Published: March 2024

The aim of this study was to develop a plant-based fermented beverage from discarded bread flour and to analyze its characteristics as a novel functional product. Eight cereal-based probiotic beverages were produced by inoculating discarded bread flour with a monoculture of Lactobacillus rhamnosus or a co-culture consisting of lactic acid bacteria and Bifidobacterium. Two additional factors, namely, the addition of amylolytic enzymes and matrix desalting, were studied alongside the type of culture. The organic acid content and microbial growth were monitored during fermentation and storage (15 and 21 days). Proximal composition, gamma-aminobutyric acid, and volatile compounds were measured in the final product. Sensory analysis was only conducted on the enzymatically treated samples. The estimated shelf life of the bread beverage was 15 days. The variables studied significantly influenced the amountof organic acids and specific volatile compounds responsible for the aroma of fermented beverages. The beverage produced via co-culturing was preferred by consumers in the sensory test.
Effects of Viable and Heat-Inactivated Bifidobacterium longum D42 on Proliferation and Apoptosis of HT-29 Human Colon Cancer Cells
Authors: Zhang Yifan , Cao Ting , Wang Yuqi , Yang Rui , Han Yu , Li Shuang , Liu Deyu , Yue Yingxue , Cao Yun , Li Bailiang , Wang Song , Huo Guicheng
Published: March 2024

Bifidobacterium longum is a common probiotic; both viable and heat-inactivated Bifidobacterium longum have many probiotic effects, such as anticancer effects. But some mechanisms of anticancer effects are still unclear, especially for heat-inactivated probiotics. In this study, we analyzed the effects of viable and heat-inactivated Bifidobacterium longum D42 on human colon cancer cells (HT-29). Cell proliferation, membrane permeability and apoptosis were detected by using the CCK-8 method, LDH method and Annexin V-FITC/PI kits. The ROS level and mitochondrial membrane potential were examined using the fluorescent probes DCFH-DA and JC-1. Real-time fluorescence quantitative PCR (RT-qPCR) and Western blot were used to detect the expression of mitochondrial apoptosis pathway genes and proteins. The results showed that viable and heat-inactivated Bifidobacterium longum D42 at concentrations of 1 × 106 CFU/mL significantly inhibited the proliferation of and increased the level of LDH release of HT-29 colon cancer cells. We found that they could increase the apoptosis rate of HT-29 cells. Moreover, they could also induce apoptosis by inducing cells to produce ROS and destroying the mitochondrial membrane potential of cells. Further studies found that they could increase the mRNA transcription and protein expression levels of the Caspase-3, Caspase-9 and Bax genes in cells, and reduce the mRNA transcription and protein expression levels of the Bcl-2 gene. In summary, our findings revealed that viable and heat-inactivated Bifidobacterium longum D42 have inhibitory effects on proliferation and promote the apoptosis of human colon cancer cells, and also have certain adjuvant drug therapeutic effects and have potential application value in the adjuvant treatment of colon cancer.
The Health History of First-Degree Relatives’ Dyslipidemia Can Affect Preferences and Intentions following the Return of Genomic Results for Monogenic Familial Hypercholesterolemia
Authors: Tokutomi Tomoharu , Yoshida Akiko , Fukushima Akimune , Yamamoto Kayono , Ishigaki Yasushi , Kawame Hiroshi , Fuse Nobuo , Nagami Fuji , Suzuki Yoichi , Sakurai-Yageta Mika , Uruno Akira , Suzuki Kichiya , Tanno Kozo , Ohmomo Hideki , Shimizu Atsushi , Yamamoto Masayuki , Sasaki Makoto
Published: March 2024

Genetic testing is key in modern healthcare, particularly for monogenic disorders such as familial hypercholesterolemia. This Tohoku Medical Megabank Project study explored the impact of first-degree relatives’ dyslipidemia history on individual responses to familial hypercholesterolemia genomic results. Involving 214 participants and using Japan’s 3.5KJPN genome reference panel, the study assessed preferences and intentions regarding familial hypercholesterolemia genetic testing results. The data revealed a significant inclination among participants with a family history of dyslipidemia to share their genetic test results, with more than 80% of participants intending to share positive results with their partners and children and 98.1% acknowledging the usefulness of positive results for personal health management. The study underscores the importance of family health history in genetic-testing perceptions, highlighting the need for family-centered approaches in genetic counseling and healthcare. Notable study limitations include the regional scope and reliance on questionnaire data. The study results emphasize the association between family health history and genetic-testing attitudes and decisions.
Enhancing Productivity and Improving Nutritional Quality of Subtropical and Temperate Leafy Vegetables in Tropical Greenhouses and Indoor Farming Systems
Authors: He Jie
Published: March 2024

The total land used for land-based food farms is less than 1% in Singapore. As a result, more than 90% of Singapore’s food needs are imported. To strengthen food security, Singapore has set a target to develop the capability and capacity of the agri-food industry to locally produce 30% of its nutritional needs by 2030. To achieve this goal, technology is the key to helping farms to “grow more with less”. This review first discusses how aeroponic systems have been adapted for growing all kinds of leafy vegetables in the tropics through the manipulation of root-zone temperature and heat priming to save power energy. Growing vegetable crops indoors and in greenhouses not only allows the growers to achieve high productivity but also enables them to enhance nutritional values. The second part of this paper emphasizes how to achieve substantial yield through deficit irrigation with higher nutritional quality in a cost-effective manner. Growing crops vertically has become increasingly popular, as it increases land use. We establish a commercially viable LED-integrated aeroponic system to grow vegetables vertically. The last part of the paper discusses the impacts of LED spectral quality, quantity, and duration on vegetable production.
Unveiling the Hidden Regulators: The Impact of lncRNAs on Zoonoses
Authors: Xu Bojie , He Yujuan , Yang Ruicheng , Li Junmin , Wang Xiangru
Published: March 2024

Zoonoses are diseases and infections naturally transmitted between humans and vertebrate animals. They form the dominant group of diseases among emerging infectious diseases and represent critical threats to global health security. This dilemma is largely attributed to our insufficient knowledge of the pathogenesis regarding zoonotic spillover. Long non-coding RNAs (lncRNAs) are transcripts with limited coding capacity. Recent technological advancements have enabled the identification of numerous lncRNAs in humans, animals, and even pathogens. An increasing body of literature suggests that lncRNAs function as key regulators in zoonotic infection. They regulate immune-related epigenetic, transcriptional, and post-transcriptional events across a broad range of organisms. In this review, we discuss the recent research progress on the roles of lncRNAs in zoonoses. We address the classification and regulatory mechanisms of lncRNAs in the interaction between host and zoonotic pathogens. Additionally, we explore the surprising function of pathogen-derived lncRNAs in mediating the pathogenicity and life cycle of zoonotic bacteria, viruses, and parasites. Understanding how these lncRNAs influence the zoonotic pathogenesis will provide important therapeutic insights to the prevention and control of zoonoses.
Have All of the Phytohormonal Properties of Melatonin Been Verified?
Authors: Park Woong June
Published: March 2024

Melatonin is a ubiquitous regulator in plants and performs a variety of physiological roles, including resistance to abiotic stress, regulation of growth and development, and enhancement of plant immunity. Melatonin exhibits the characteristics of a phytohormone with its pleiotropic effects, biosynthesis, conjugation, catabolism, effective concentration, and the shape and location of its dose–response curves. In addition, CAND2/PMTR1, a phytomelatonin receptor candidate belonging to the G protein-coupled receptors (GPCRs), supports the concept of melatonin as a phytohormone. However, the biochemistry of plant melatonin receptors needs to be further characterized. In particular, some of the experimental findings to date cannot be explained by known GPCR signaling mechanisms, so further studies are needed to explore the possibility of novel signaling mechanisms.
Development and Evaluation of an In-House Real-Time RT-PCR Targeting nsp10 Gene for SARS-CoV-2 Detection
Authors: Yip Cyril Chik-Yan , Poon Jane Hau-Ching , Leung Kit-Hang , Chan Wan-Mui , Ip Jonathan Daniel , Chu Allen Wing-Ho , Cheng Vincent Chi-Chung , Yuen Kwok-Yung , To Kelvin Kai-Wang
Published: March 2024

The emergence of SARS-CoV-2 mutations poses significant challenges to diagnostic tests, as these mutations can reduce the sensitivity of commonly used RT-PCR assays. Therefore, there is a need to design diagnostic assays with multiple targets to enhance sensitivity. In this study, we identified a novel diagnostic target, the nsp10 gene, using nanopore sequencing. Firstly, we determined the analytical sensitivity and specificity of our COVID-19-nsp10 assay. The COVID-19-nsp10 assay had a limit of detection of 74 copies/mL (95% confidence interval: 48–299 copies/mL) and did not show cross-reactivity with other respiratory viruses. Next, we determined the diagnostic performance of the COVID-19-nsp10 assay using 261 respiratory specimens, including 147 SARS-CoV-2-positive specimens belonging to the ancestral strain and Alpha, Beta, Gamma, Delta, Mu, Eta, Kappa, Theta and Omicron lineages. Using a LightMix E-gene RT-PCR assay as the reference method, the diagnostic sensitivity and specificity of the COVID-19-nsp10 assay were found to be 100%. The median Cp values for the LightMix E-gene RT-PCR and our COVID-19-nsp10 RT-PCR were 22.48 (range: 12.95–36.60) and 25.94 (range 16.37–36.87), respectively. The Cp values of the COVID-19-nsp10 RT-PCR assay correlated well with those of the LightMix E-gene RT-PCR assay (Spearman’s ρ = 0.968; p < 0.0001). In conclusion, nsp10 is a suitable target for a SARS-CoV-2 RT-PCR assay.
Tocotrienols Prevent the Decline of Learning Ability in High-Fat, High-Sucrose Diet-Fed C57BL/6 Mice
Authors: Kato Yugo , Ben Junhyoku , Noto Atsuto , Kashiwaya Shuntaro , Aoki Yoshinori , Watanabe Nobuo , Tsumoto Hiroki , Miura Yuri , Fukui Koji
Published: March 2024

Obesity has been increasing worldwide and is well-known as a risk factor for cognitive decline. It has been reported that oxidative stress in the brain is deeply involved in cognitive dysfunction in rodent models. While there are many studies on oxidation in the liver and adipose tissue of obese mice, the relationship between obesity-induced cognitive dysfunction and brain oxidation has not been elucidated. Here, we show that obesity induced by a high-fat, high-sucrose diet (HFSD) alters cognitive function in C57BL/6 male mice, and it may involve the acceleration of brain oxidation. Tocotrienols (T3s), which are members of the vitamin E family, can prevent HFSD-induced cognitive changes. To elucidate these mechanisms, respiratory metabolism, locomotor activity, temperature around brown adipose tissue, and protein profiles in the cerebrum cortex were measured. Contrary to our expectation, respiratory metabolism was decreased, and temperature around brown adipose tissue was increased in the feeding of HFSD. The proteins that regulate redox balance did not significantly change, but 12 proteins, which were changed by HFSD feeding and not changed by T3s-treated HFSD compared to control mice, were identified. Our results indicated that HFSD-induced obesity decreases mouse learning ability and that T3s prevent its change. Additionally, feeding of HFSD significantly increased brain oxidation. However, further study is needed to elucidate the mechanisms of change in oxidative stress in the brain by obesity.