bluuebunny/crossref_metadata_2025 huggingface dataset:
Particle Films Combined with Propolis Have Positive Effects in Reducing Bactrocera oleae Attacks on Olive Fruits
Authors: Daher Elissa , Rondoni Gabriele , Cinosi Nicola , Conti Eric , Famiani Franco
Published: March 2023

The olive fruit fly, Bactrocera oleae, is a major pest of olive trees in several areas of the world. Testing novel preventive methods against B. oleae infestations is paramount. The use of particle film in eluding B. oleae and avoiding oviposition is one of the main strategies adopted by olive growers; however, choices are often limited to kaolin. Under field conditions, we tested the efficacy of novel compounds, including particle films, for their effectiveness as oviposition deterrents against B. oleae. The trial was conducted from July to October 2021 in an olive orchard located in central Italy. One olive variety, Borgiona, was selected and sprayed with propolis, rock powder, kaolin, the mixture of propolis and rock powder, the mixture of propolis and kaolin and water (control). Laboratory analyses were conducted to study the effects of the treatments on the fruit maturity index. As per the field trial, the rock powder and propolis mixture caused a reduction of B. oleae infestation with respect to the control (water), similarly to kaolin. Moreover, the mixture of kaolin and propolis exhibited the best results among all treatments. When mixed with propolis, particle films showed higher protection from B. oleae than when applied alone, suggesting a synergistic effect, and demonstrating an interesting role of propolis as an adjuvant. No delay in fruit maturity was noticed. Our results indicate that the tested products have the potential to be incorporated into management programs of B. oleae, although possible side effects on olive physiology require additional investigations.
Synergistic Effect of Pd Co-Catalyst and rGO–TiO2 Hybrid Support for Enhanced Photoreforming of Oxygenates
Authors: Majeed Imran , Arif Ayesha , Idrees Afifa , Ullah Hafeez , Ali Hassan , Mehmood Arshad , Rashid Ashi , Nadeem Muhammad Arif , Nadeem Muhammad Amtiaz
Published: March 2023

Photoreforming biomass-derived waste such as glycerol into hydrogen fuel is a renewable hydrogen generation technology that has the potential to become important due to unavoidable CO2 production during methane steam reforming. Despite tremendous efforts, the challenge of developing highly active photocatalysts at a low cost still remains elusive. Here, we developed a novel photocatalyst with a hybrid support comprising reduced graphene oxide (rGO) and TiO2 nanorods (TNR). rGO in the hybrid support not only performed as an excellent scavenger of electrons from the semiconductor conduction band due to its suitable electrochemical potential, but also acted as an electron transport highway to the metal co-catalyst, which otherwise is not possible by simply increasing metal loading due to the shadowing effect. A series of hybrid supports with different TNR and rGO ratios were prepared by the deposition method. Pd nanoparticles were deposited over hybrid support through the chemical reduction method. Pd/rGO-TNRs photocatalyst containing 4 wt.% rGO contents in the support and 1 wt.% nominal Pd loading demonstrated hydrogen production activity ~41 mmols h−1g−1, which is 4 and 40 times greater than benchmark Au/TiO2 and pristine P25. The findings of this works provide a new strategy in optimizing charge extraction from TiO2, which otherwise has remained impossible due to a fixed tradeoff between metal loading and the detrimental shadowing effect.
Utilizing NDWI, MNDWI, SAVI, WRI, and AWEI for Estimating Erosion and Deposition in Ping River in Thailand
Authors: Laonamsai Jeerapong , Julphunthong Phongthorn , Saprathet Thanat , Kimmany Bounhome , Ganchanasuragit Tammarat , Chomcheawchan Phornsuda , Tomun Nattapong
Published: March 2023

The Ping River, located in northern Thailand, is facing various challenges due to the impacts of climate change, dam operations, and sand mining, leading to riverbank erosion and deposition. To monitor the riverbank erosion and accretion, this study employs remote sensing and GIS technology, utilizing five water indices: the Normalized Difference Water Index (NDWI), Modified Normalized Difference Water Index (MNDWI), Soil-Adjusted Vegetation Index (SAVI), Water Ratio Index (WRI), and Automated Water Extraction Index (AWEI). The results from each water index were comparable, with an accuracy ranging from 79.10 to 94.53 percent and analytical precision between 96.05 and 100 percent. The AWEI and WRI streams showed the highest precision out of the five indices due to their larger total surface water area. Between 2015 and 2022, the riverbank of the Ping River saw 5.18 km2 of erosion. Conversely, the morphological analysis revealed 5.55 km2 of accretion in low-lying river areas. The presence of riverbank stabilizing structures has resulted in accretion being greater than erosion, leading to the formation of riverbars along the Ping River. The presence of water hyacinth, narrow river width, and different water levels between the given periods may impact the accuracy of retrieved river areas.
Extract of Aster koraiensis Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action
Authors: Lee Seung-Eun , Park Saetbyeol , Jang Gwi Yeong , Lee Jeonghoon , Moon Minho , Ji Yun-Jeong , Jung Ji Wook , Nam Yunkwon , Shin Soo Jung , Lee Yunji , Choi Jehun , Kim Dong Hwi
Published: March 2023

Aster koraiensis Nakai (AK) leaf reportedly ameliorates health problems, such as diabetes. However, the effects of AK on cognitive dysfunction or memory impairment remain unclear. This study investigated whether AK leaf extract could attenuate cognitive impairment. We found that AK extract reduced the production of nitric oxide (NO), tumour necrosis factor (TNF)-α, phosphorylated-tau (p-tau), and the expression of inflammatory proteins in lipopolysaccharide- or amyloid-β-treated cells. AK extract exhibited inhibitory activity of control specific binding on N-methyl-D-aspartate (NMDA) receptors. Scopolamine-induced AD models were used chronically in rats and acutely in mice. Relative to negative controls (NC), hippocampal choline acetyltransferase (ChAT) and B-cell lymphoma 2 (Bcl2) activity was increased in rats chronically treated with scopolamine and fed an AK extract-containing diet. In the Y-maze test, spontaneous alterations were increased in the AK extract-fed groups compared to NC. Rats administered AK extract showed increased escape latency in the passive avoidance test. In the hippocampus of rats fed a high-AK extract diet (AKH), the expression of neuroactive ligand–receptor interaction-related genes, including Npy2r, Htr2c, and Rxfp1, was significantly altered. In the Morris water maze assay of mice acutely treated with scopolamine, the swimming times in the target quadrant of AK extract-treated groups increased significantly to the levels of the Donepezil and normal groups. We used Tg6799 Aβ-overexpressing 5XFAD transgenic mice to investigate Aβ accumulation in animals. In the AD model using 5XFAD, the administration of AK extract decreased amyloid-β (Aβ) accumulation and increased the number of NeuN antibody-reactive cells in the subiculum relative to the control group. In conclusion, AK extract ameliorated memory dysfunction by modulating ChAT activity and Bcl2-related anti-apoptotic pathways, affecting the expression of neuroactive ligand–receptor interaction-related genes and inhibiting Aβ accumulation. Therefore, AK extract could be a functional material improving cognition and memory.
Combined Inhibition of Hedgehog and HDAC6: In Vitro and In Vivo Studies Reveal a New Role for Lysosomal Stress in Reducing Glioblastoma Cell Viability
Authors: Pezzotta Alex , Brioschi Loredana , Carbone Sabrina , Mazzoleni Beatrice , Bontempi Vittorio , Monastra Federica , Mauri Laura , Marozzi Anna , Mione Marina , Pistocchi Anna , Viani Paola
Published: March 2023

Glioblastoma multiforme (GBM) is the most common and malignant brain tumor in adults. The invasiveness and the rapid progression that characterize GBM negatively impact patients’ survival. Temozolomide (TMZ) is currently considered the first-choice chemotherapeutic agent. Unfortunately, over 50% of patients with GBM do not respond to TMZ treatment, and the mutation-prone nature of GBM enables the development of resistance mechanisms. Therefore, efforts have been devoted to the dissection of aberrant pathways involved in GBM insurgence and resistance in order to identify new therapeutic targets. Among them, sphingolipid signaling, Hedgehog (Hh) pathway, and the histone deacetylase 6 (HDAC6) activity are frequently dysregulated and may represent key targets to counteract GBM progression. Given the positive correlation between Hh/HDAC6/sphingolipid metabolism in GBM, we decided to perform a dual pharmacological inhibition of Hh and HDAC6 through cyclopamine and tubastatin A, respectively, in a human GMB cell line and zebrafish embryos. The combined administration of these compounds elicited a more significant reduction of GMB cell viability than did single treatments in vitro and in cells orthotopically transplanted in the zebrafish hindbrain ventricle. We demonstrated, for the first time, that the inhibition of these pathways induces lysosomal stress which results in an impaired fusion of lysosomes with autophagosomes and a block of sphingolipid degradation in GBM cell lines. This condition, which we also recapitulated in zebrafish embryos, suggests an impairment of lysosome-dependent processes involving autophagy and sphingolipid homeostasis and might be instrumental in the reduction of GBM progression.
Modulation of Endothelial Function by TMAO, a Gut Microbiota-Derived Metabolite
Authors: Querio Giulia , Antoniotti Susanna , Geddo Federica , Levi Renzo , Gallo Maria Pia
Published: March 2023

Endothelial function is essential in the maintenance of systemic homeostasis, whose modulation strictly depends on the proper activity of tissue-specific angiocrine factors on the physiopathological mechanisms acting at both single and multi-organ levels. Several angiocrine factors take part in the vascular function itself by modulating vascular tone, inflammatory response, and thrombotic state. Recent evidence has outlined a strong relationship between endothelial factors and gut microbiota-derived molecules. In particular, the direct involvement of trimethylamine N-oxide (TMAO) in the development of endothelial dysfunction and its derived pathological outcomes, such as atherosclerosis, has come to light. Indeed, the role of TMAO in the modulation of factors strictly related to the development of endothelial dysfunction, such as nitric oxide, adhesion molecules (ICAM-1, VCAM-1, and selectins), and IL-6, has been widely accepted. The aim of this review is to present the latest studies that describe a direct role of TMAO in the modulation of angiocrine factors primarily involved in the development of vascular pathologies.
Mesenchymal Stem Cells from COPD Patients Are Capable of Restoring Elastase-Induced Emphysema in a Murine Experimental Model
Authors: Río Carlos , Jahn Andreas K. , Martin-Medina Aina , Calvo Bota Alba Marina , De Francisco Casado Mª Teresa , Pont Antona Pere Joan , Gigirey Castro Orlando , Carvajal Ángel Francisco , Villena Portella Cristina , Gómez Bellvert Cristina , Iglesias Amanda , Calvo Benito Javier , Gayà Puig Antoni , Ortiz Luis A. , Sala-Llinàs Ernest
Published: March 2023

COPD is a chronic lung disease that affects millions of people, declining their lung function and impairing their life quality. Despite years of research and drug approvals, we are still not capable of halting progression or restoring normal lung function. Mesenchymal stem cells (MSC) are cells with extraordinary repair capacity, and MSC-based therapy brings future hope for COPD treatment, although the best source and route of administration are unclear. MSC from adipose tissue (AD-MSC) represents an option for autologous treatment; however, they could be less effective than donor MSC. We compared in vitro behavior of AD-MSC from COPD and non-COPD individuals by migration/proliferation assay, and tested their therapeutic potential in an elastase mouse model. In addition, we tested intravenous versus intratracheal routes, inoculating umbilical cord (UC) MSC and analyzed molecular changes by protein array. Although COPD AD-MSC have impaired migratory response to VEGF and cigarette smoke, they were as efficient as non-COPD in reducing elastase-induced lung emphysema. UC-MSC reduced lung emphysema regardless of the administration route and modified the inflammatory profile in elastase-treated mice. Our data demonstrate equal therapeutic potential of AD-MSC from COPD and non-COPD subjects in the pre-clinical model, thus supporting their autologous use in disease.
Transcriptome Reveals the Molecular Mechanism of the ScALDH21 Gene from the Desert Moss Syntrichia caninervis Conferring Resistance to Salt Stress in Cotton
Authors: Yang Honglan , Yang Qilin , Zhang Dawei , Wang Jiancheng , Cao Ting , Bozorov Tohir A. , Cheng Lihua , Zhang Daoyuan
Published: March 2023

The desert moss Syntrichia caninervis has proven to be an excellent plant material for mining resistance genes. The aldehyde dehydrogenase 21 (ScALDH21) gene from S. caninervis has been shown to confer tolerance to salt and drought, but it is unclear how the transgene ScALDH21 regulates tolerance to abiotic stresses in cotton. In the present work, we studied the physiological and transcriptome analyses of non-transgenic (NT) and transgenic ScALDH21 cotton (L96) at 0 day, 2 days, and 5 days after salt stress. Through intergroup comparisons and a weighted correlation network analysis (WGCNA), we found that there were significant differences between NT and L96 cotton in the plant hormone, Ca2+, and mitogen-activated protein kinase (MAPK) signaling pathways as well as for photosynthesis and carbohydrate metabolism. Overexpression of ScALDH21 significantly increased the expression of stress-related genes in L96 compared to NT cotton under both normal growth and salt stress conditions. These data suggest that the ScALDH21 transgene can scavenge more reactive oxygen species (ROS) in vivo relative to NT cotton and improve cotton resistance to salt stress by increasing the expression of stress-responsive genes, responding quickly to stress stimuli, enhancing photosynthesis and improving carbohydrate metabolism. Therefore, ScALDH21 is a promising candidate gene to improve resistance to salt stress, and the application of this gene in cotton provides new insights into molecular plant breeding.
Hot Melt Extrusion as an Effective Process in the Development of Mucoadhesive Tablets Containing Scutellariae baicalensis radix Extract and Chitosan Dedicated to the Treatment of Oral Infections
Authors: Paczkowska-Walendowska Magdalena , Miklaszewski Andrzej , Szymanowska Daria , Skalicka-Woźniak Krystyna , Cielecka-Piontek Judyta
Published: March 2023

Hot Melt Extrusion (HME) technology was developed to obtain blends containing lyophilized Scutellariae baicalensis root extract and chitosan in order to improve the rheological properties of the obtained blends, including tableting and compressibility properties. (Hydroxypropyl)methyl cellulose (HPMC) in 3 different ratios was used as amorphous matrix formers. The systems were characterized using X-ray powder diffraction (PXRD), Fourier Transform Infrared Spectroscopy with Attenuated Total Reflectance (FTIR-ATR), and in vitro release, permeability, and microbiological activity studies. Then, the extrudates were used to prepare tablets in order to give them the appropriate pharmaceutical form. HPMC-based systems released baicalin more slowly, resulting in delayed peaks in the acceptor fluid. This behavior can be explained by the fact that HPMC swells significantly, and the dissolved substance must have diffused through the polymer network before being released. The best tabletability properties are provided by the formulation containing the extrudate with lyophilized extract HPMC 50:50 w/w. These tablets offer a valuable baicalin release profile while maintaining good mucoadhesive properties that condition the tablet’s retention in the application site and the effectiveness of therapy.
Monoterpenoid Epoxidiol Ameliorates the Pathological Phenotypes of the Rotenone-Induced Parkinson’s Disease Model by Alleviating Mitochondrial Dysfunction
Authors: Aleksandrova Yulia , Chaprov Kirill , Podturkina Alexandra , Ardashov Oleg , Yandulova Ekaterina , Volcho Konstantin , Salakhutdinov Nariman , Neganova Margarita
Published: March 2023

Parkinson’s disease is the second most common neurodegenerative disease. Unfortunately, there is still no definitive disease-modifying therapy. In our work, the antiparkinsonian potential of trans-epoxide (1S,2S,3R,4S,6R)-1-methyl-4-(prop-1-en-2-yl)-7-oxabicyclo [4.1.0]heptan-2,3-diol (E-diol) was analyzed in a rotenone-induced neurotoxicity model using in vitro, in vivo and ex vivo approaches. It was conducted as part of the study of the mitoprotective properties of the compound. E-diol has been shown to have cytoprotective properties in the SH-SY5Y cell line exposed to rotenone, which is associated with its ability to prevent the loss of mitochondrial membrane potential and restore the oxygen consumption rate after inhibition of the complex I function. Under the conditions of rotenone modeling of Parkinson’s disease in vivo, treatment with E-diol led to the leveling of both motor and non-motor disorders. The post-mortem analysis of brain samples from these animals demonstrated the ability of E-diol to prevent the loss of dopaminergic neurons. Moreover, that substance restored functioning of the mitochondrial respiratory chain complexes and significantly reduced the production of reactive oxygen species, preventing oxidative damage. Thus, E-diol can be considered as a new potential agent for the treatment of Parkinson’s disease.
Resmetirom Ameliorates NASH-Model Mice by Suppressing STAT3 and NF-κB Signaling Pathways in an RGS5-Dependent Manner
Authors: Wang Xiaojing , Wang Liangjing , Geng Lin , Tanaka Naoki , Ye Bin
Published: March 2023

Resmetirom, a liver-directed, orally active agonist of THR-β, could play a favorable role in treating NASH, but little is known about the underlying mechanism. A NASH cell model was established to test the preventive effect of resmetirom on this disease in vitro. RNA-seq was used for screening, and rescue experiments were performed to validate the target gene of the drug. A NASH mouse model was used to further elucidate the role and the underlying mechanism of resmetirom. Resmetirom effectively eliminated lipid accumulation and decreased triglyceride (TG) levels. In addition, repressed RGS5 in the NASH model could be recovered by resmetirom treatment. The silencing of RGS5 effectively impaired the role of resmetirom. In the NASH mouse model, obvious gray hepatization, liver fibrosis and inflammation, and increased macrophage infiltration were observed in liver tissues, while resmetirom almost returned them to normal conditions as observed in the control group. Pathological experimental data also confirmed that resmetirom has great potential in NASH treatment. Finally, RGS5 expression was suppressed in the NASH mouse model, but it was upregulated by resmetirom treatment, while the STAT3 and NF-κB signaling pathways were activated in NASH but inhibited by the agent. Resmetirom could improve NASH by recovering RGS5 expression and subsequently inactivating the STAT3 and NF-κB signaling pathways.
Blood-Based Biomarkers as Prognostic Factors of Recurrent Disease after Radical Cystectomy: A Systematic Review and Meta-Analysis
Authors: Ofner Heidemarie , Laukhtina Ekaterina , Hassler Melanie R. , Shariat Shahrokh F.
Published: March 2023

Survival outcomes after radical cystectomy (RC) for bladder cancer (BCa) have not improved in recent decades; nevertheless, RC remains the standard treatment for patients with localized muscle-invasive BCa. Identification of the patients most likely to benefit from RC only versus a combination with systemic therapy versus systemic therapy first/only and bladder-sparing is needed. This systematic review and meta-analysis pools the data from published studies on blood-based biomarkers to help prognosticate disease recurrence after RC. A literature search on PubMed and Scopus was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement. Articles published before November 2022 were screened for eligibility. A meta-analysis was performed on studies investigating the association of the neutrophil-to-lymphocyte ratio (NLR), the only biomarker with sufficient data, with recurrence-free survival. The systematic review identified 33 studies, and 7 articles were included in the meta-analysis. Our results demonstrated a statistically significant correlation between elevated NLR and an increased risk of disease recurrence (HR 1.26; 95% CI 1.09, 1.45; p = 0.002) after RC. The systematic review identified various other inflammatory biomarkers, such as interleukin-6 or the albumin-to-globulin ratio, which have been reported to have a prognostic impact on recurrence after RC. Besides that, the nutritional status, factors of angiogenesis and circulating tumor cells, and DNA seem to be promising tools for the prognostication of recurrence after RC. Due to the high heterogeneity between the studies and the different cut-off values of biomarkers, prospective and validation trials with larger sample sizes and standardized cut-off values should be conducted to strengthen the approach in using biomarkers as a tool for risk stratification in clinical decision-making for patients with localized muscle-invasive BCa.
Non-Small Cell Lung Cancer Treatment with Molecularly Targeted Therapy and Concurrent Radiotherapy—A Review
Authors: Król Katarzyna , Mazur Anna , Stachyra-Strawa Paulina , Grzybowska-Szatkowska Ludmiła
Published: March 2023

Lung cancer is the leading cause of death worldwide for both men and women. Surgery can be offered as a radical treatment at stages I and II and selected cases of stage III (III A). Whereas at more advanced stages, combined modalities of treatment are applied: radiochemotherapy (IIIB) and molecularly targeted treatment (small molecule tyrosine kinase inhibitors, VEGF receptor inhibitors, monoclonal antibodies, and immunological treatment with monoclonal antibodies). Combination treatment, composed of radiotherapy and molecular therapy, is increasingly employed in locally advanced and metastatic lung cancer management. Recent studies have indicated a synergistic effect of such treatment and modification of immune response. The combination of immunotherapy and radiotherapy may result in the enhancement of the abscopal effect. Anti-angiogenic therapy, in combination with RT, is associated with high toxicity and should be not recommended. In this paper, the authors discuss the role of molecular treatment and the possibility of its concurrent use with radiotherapy in non-small cell lung cancer (NSCLC).
Comparing the Effects of Rocaglates on Energy Metabolism and Immune Modulation on Cells of the Human Immune System
Authors: Schiffmann Susanne , Henke Marina , Seifert Michelle , Ulshöfer Thomas , Roser Luise A. , Magari Francesca , Wendel Hans-Guido , Grünweller Arnold , Parnham Michael J.
Published: March 2023

A promising new approach to broad spectrum antiviral drugs is the inhibition of the eukaryotic translation initiation factor 4A (elF4A), a DEAD-box RNA helicase that effectively reduces the replication of several pathogenic virus types. Beside the antipathogenic effect, modulation of a host enzyme activity could also have an impact on the immune system. Therefore, we performed a comprehensive study on the influence of elF4A inhibition with natural and synthetic rocaglates on various immune cells. The effect of the rocaglates zotatifin, silvestrol and CR-31-B (−), as well as the nonactive enantiomer CR-31-B (+), on the expression of surface markers, release of cytokines, proliferation, inflammatory mediators and metabolic activity in primary human monocyte-derived macrophages (MdMs), monocyte-derived dendritic cells (MdDCs), T cells and B cells was assessed. The inhibition of elF4A reduced the inflammatory potential and energy metabolism of M1 MdMs, whereas in M2 MdMs, drug-specific and less target-specific effects were observed. Rocaglate treatment also reduced the inflammatory potential of activated MdDCs by altering cytokine release. In T cells, the inhibition of elF4A impaired their activation by reducing the proliferation rate, expression of CD25 and cytokine release. The inhibition of elF4A further reduced B-cell proliferation, plasma cell formation and the release of immune globulins. In conclusion, the inhibition of the elF4A RNA helicase with rocaglates suppressed the function of M1 MdMs, MdDCs, T cells and B cells. This suggests that rocaglates, while inhibiting viral replication, may also suppress bystander tissue injury by the host immune system. Thus, dosing of rocaglates would need to be adjusted to prevent excessive immune suppression without reducing their antiviral activity.
Circular RNA CircCDKN2B−AS_006 Promotes the Tumor-like Growth and Metastasis of Rheumatoid Arthritis Synovial Fibroblasts by Targeting the miR−1258/RUNX1 Axis
Authors: Xu Yayun , Zai Zhuoyan , Lu Zheng , Zhang Tao , Wang Longfei , Qian Xuewen , Tao Jingjing , Peng Xiaoqing , Zhang Yihao , Chen Feihu
Published: March 2023

Rheumatoid arthritis (RA) is an autoimmune polyarthritis in which synovial fibroblasts (SFs) play a major role in cartilage and bone destruction through tumor−like proliferation, migration, and invasion. Circular RNAs (circRNAs) have emerged as vital regulators for tumor progression. However, the regulatory role, clinical significance, and underlying mechanisms of circRNAs in RASF tumor−like growth and metastasis remain largely unknown. Differentially expressed circRNAs in synovium samples from patients with RA and patients with joint trauma were identified via RNA sequencing. Subsequently, in vitro and in vivo experiments were performed to investigate the functional roles of circCDKN2B−AS_006 in RASF proliferation, migration, and invasion. CircCDKN2B−AS_006 was upregulated in synovium samples from patients with RA and promoted the tumor-like proliferation, migration, and invasion of RASFs. Mechanistically, circCDKN2B−AS_006 was shown to regulate the expression of runt−related transcription factor 1 (RUNX1) by sponging miR-1258, influencing the Wnt/β−catenin signaling pathway, and promoting the epithelial−to−mesenchymal transition (EMT) in RASFs. Moreover, in the collagen−induced arthritis (CIA) mouse model, intra−articular injection of lentivirus−shcircCDKN2B−AS_006 was capable of alleviating the severity of arthritis and inhibiting the aggressive behaviors of SFs. Furthermore, the correlation analysis results revealed that the circCDKN2B−AS_006/miR−1258/RUNX1 axis in the synovium was correlated with the clinical indicators of RA patients. CircCDKN2B−AS_006 promoted the proliferation, migration, and invasion of RASFs by modulating the miR−1258/RUNX1 axis.
A Study of Modern Eco-Friendly Composite (Geopolymer) Based on Blast Furnace Slag Compared to Conventional Concrete Using the Life Cycle Assessment Approach
Authors: Esparham Alireza , Vatin Nikolai Ivanovich , Kharun Makhmud , Hematibahar Mohammad
Published: March 2023

By posing the question of what will be the definition of sustainable development in the future, it can almost be seen that the principle of “no waste” and the production of new materials with less of a negative environmental impact will have a high priority. To further develop environmentally friendly materials, it is necessary to know about the environmental drivers of new materials as well as to evaluate the environmental effects of conventional materials in construction. According to the definitions of sustainable development and sustainable materials, materials with characteristics such as having low energy consumption, sufficient durability, good physical and chemical properties, while simultaneously reducing pollution should be used. Geopolymer materials may be a reasonable option. In this research, two production processes based on blast furnace slag and ordinary concrete (Portland cement) for one cubic meter of geopolymer concrete have been investigated. To investigate, inputs (materials and energy) and outputs (relevant environmental pollutants) of both systems were determined and a life cycle assessment (LCA) was measured using the Center of Environmental Science of Leiden University (CML) and cumulative exergy demand (CED) quantification methods of SimaPro V.9 software. The results showed that the production system of one cubic meter of conventional concrete has maximum environmental effects in all classes except in the destruction of the ozone layer, and the system of producing one cubic meter of geopolymer concrete based on slag has much less environmental effects than the normal concrete system. It also consumes 62% less directly during its lifetime. As a result, geopolymer concrete may be a suitable alternative to traditional concrete as a sustainable material.
The Electronic Nature of Cationic Group 10 Ylidyne Complexes
Authors: Maurer Leonard R. , Rump Jens , Filippou Alexander C.
Published: March 2023

We report a broad theoretical study on [(PMe3)3MER]+ complexes, with M = Ni, Pd, Pt, E = C, Si, Ge, Sn, Pb, and R = ArMes, Tbb, (ArMes = 2,6-dimesitylphenyl; Tbb = C6H2-2,6-[CH(SiMe3)2]2-4-tBu). A few years ago, our group succeeded in obtaining heavier homologues of cationic group 10 carbyne complexes via halide abstraction of the tetrylidene complexes [(PMe3)3M=E(X)R] (X = Cl, Br) using a halide scavenger. The electronic structure and the M-E bonds of the [(PMe3)3MER]+ complexes were analyzed utilizing quantum-chemical tools, such as the Pipek–Mezey orbital localization method, the energy decomposition analysis (EDA), and the extended-transition state method with natural orbitals of chemical valence (ETS-NOCV). The carbyne, silylidyne complexes, and the germylidyne complex [(PMe3)3NiGeArMes]+ are suggested to be tetrylidyne complexes featuring donor–acceptor metal tetrel triple bonds, which are composed of two strong π(M→E) and one weaker σ(E→M) interaction. In comparison, the complexes with M = Pd, Pt; E = Sn, Pb; and R = ArMes are best described as metallotetrylenes and exhibit considerable M−E−C bending, a strong σ(M→E) bond, weakened M−E π-components, and lone pair density at the tetrel atoms. Furthermore, bond cleavage energy (BCE) and bond dissociation energy (BDE) reveal preferred splitting into [M(PMe3)3]+ and [ER] fragments for most complex cations in the range of 293.3–618.3 kJ·mol−1 and 230.4–461.6 kJ·mol−1, respectively. Finally, an extensive study of the potential energy hypersurface varying the M−E−C angle indicates the presence of isomers with M−E−C bond angles of around 95°. Interestingly, these isomers are energetically favored for M = Pd, Pt; E = Sn, Pb; and R = ArMes over the less-bent structures by 13–29 kJ·mol−1.
Cosmic-Ray Tomography for Border Security
Authors: Barnes Sarah , Georgadze Anzori , Giammanco Andrea , Kiisk Madis , Kudryavtsev Vitaly A. , Lagrange Maxime , Pinto Olin Lyod
Published: March 2023

A key task for customs workers is the interception of hazardous, illegal and counterfeit items in order to protect the health and safety of citizens. However, it is estimated that only a small fraction of cargo is inspected and an even smaller fraction of trafficked goods are detected. Today, the most widely used technology for scanning vehicles, ranging from vans and trucks to railcars, is γ ray and X-ray radiography. New technologies are required to overcome current technological shortcomings, such as the inability to detect the target material composition, the usage of harmful ionising radiation sources and the resultant low throughput. Cosmic ray tomography (CRT) is a promising technology for cargo screening. Cosmic ray muons have average energies of around 10,000 times larger than a typical X-ray and therefore can penetrate relatively large and dense materials. By analysing muon scattering, it is possible to identify materials hidden inside shielding that is too thick or deep for other imaging methods. CRT is also completely passive, exploiting naturally occurring secondary cosmic radiation, and is therefore safe for humans and animals. Contrary to conventional X-ray- or γ-ray-based imaging techniques, CRT also allows material differentiation and anomaly localisation within the cargo or vehicle through the provision of 3D images. This article reviews the current state-of-the-art technology in CRT, critically assessing the strengths and weaknesses of the method, and suggesting further directions for development.
Design of Reliable Remobilisation Finger Implants with Geometry Elements of a Triple Periodic Minimal Surface Structure via Additive Manufacturing of Silicon Nitride
Authors: Koplin Christof , Schwarzer-Fischer Eric , Zschippang Eveline , Löw Yannick Marian , Czekalla Martin , Seibel Arthur , Rörich Anna , Georgii Joachim , Güttler Felix , Yarar-Schlickewei Sinef , Kailer Andreas
Published: March 2023

When finger joints become immobile due to an accident during sports or a widespread disease such as rheumatoid arthritis, customised finger joint implants are to be created. In an automated process chain, implants will be produced from ceramic or metallic materials. Artificial intelligence-supported software is used to calculate three-dimensional models of the finger bones from two-dimensional X-ray images. Then, the individual implant design is derived from the finger model and 3D printed. The 3D printing process and the structures used are evaluated via model tests and the final implant design via a reliability calculation in a way to ensure that this is also possible via an AI process in the future. Using additive manufacturing with silicon nitride-based ceramics, model specimens and implants are produced via the lithography-based ceramic vat photopolymerisation process with full geometry or elements of triple periodic minimal surfaces structure. The model specimens are tested experimentally, and the loads are matched with a characteristic strength assuming a Weibull distribution of defects in the volume to generate and match failure probabilities. Calculated fracture forces of the silicon nitride-based ceramic structure was validated by comparison of simulation and tests, and the calculation can be used as a quality index for training of artificial intelligence in the future. The proposed method for individualized finger implant design and manufacturing may allow for correction of potential malpositions of the fingers in the future.
Noise-Induced Hearing Loss
Authors: Natarajan Nirvikalpa , Batts Shelley , Stankovic Konstantina M.
Published: March 2023

Noise-induced hearing loss (NIHL) is the second most common cause of sensorineural hearing loss, after age-related hearing loss, and affects approximately 5% of the world’s population. NIHL is associated with substantial physical, mental, social, and economic impacts at the patient and societal levels. Stress and social isolation in patients’ workplace and personal lives contribute to quality-of-life decrements which may often go undetected. The pathophysiology of NIHL is multifactorial and complex, encompassing genetic and environmental factors with substantial occupational contributions. The diagnosis and screening of NIHL are conducted by reviewing a patient’s history of noise exposure, audiograms, speech-in-noise test results, and measurements of distortion product otoacoustic emissions and auditory brainstem response. Essential aspects of decreasing the burden of NIHL are prevention and early detection, such as implementation of educational and screening programs in routine primary care and specialty clinics. Additionally, current research on the pharmacological treatment of NIHL includes anti-inflammatory, antioxidant, anti-excitatory, and anti-apoptotic agents. Although there have been substantial advances in understanding the pathophysiology of NIHL, there remain low levels of evidence for effective pharmacotherapeutic interventions. Future directions should include personalized prevention and targeted treatment strategies based on a holistic view of an individual’s occupation, genetics, and pathology.
Comparison of Periprocedural and Intermediate-Term Outcomes of TAVI in Patients with Ejection Fraction ≤ 20% vs. Patients with 20% < EF ≤ 40%
Authors: Kodra Arber , Cinelli Michael , Alexander Renita , Hamfreth Rahming , Wang Denny , Thampi Shankar , Basman Craig , Kliger Chad , Scheinerman Jacob , Pirelli Luigi
Published: March 2023

Treatment of congestive heart failure (CHF) with left ventricular (LV) systolic dysfunction and severe aortic stenosis (AS) is challenging, yet transcatheter aortic valve replacement (TAVR) has emerged as a suitable treatment option in such patients. We compared the periprocedural outcomes of TAVR in patients with an ejection fraction (EF) of ≤20% (VLEF group) to patients with an EF > 20% to ≤40% (LEF group). We included patients with severe AS and reduced LV ejection fraction (LVEF ≤ 40%) who underwent TAVR at four centers within Northwell Health between January 2016 and December 2020. Over 2000 consecutive patients were analyzed, of which 355 patients met the inclusion criteria. The primary composite endpoint was in-hospital mortality, moderate or greater paravalvular (PVL), stroke, conversion to open surgery, aortic valve re-intervention, and/or need for PPM. Secondary endpoints were length of stay, NYHA classification at 1 month and 1 year, mortality at 1 month and 1 year, mean valve gradient at 1 month, KCCQ score at 1 month, and ≥ moderate PVL at 1 month. There was no difference in the primary composite endpoint between the two groups (23.6% for VLEF vs. 25.3% for LEF, p = 0.29). During TAVR placement, 40% of patients in the VLEF group required ≥1 vasopressors for hypotension lasting ≥30 min vs. only 21% of patients in the LEF group (p < 0.01). Intra-aortic balloon pump (IABP) use during procedure was greater in the VLEF group (9% vs. 1%, p < 0.01)—all placed post TAVR. Emergency ECMO use was higher in the VLEF group as well (5% vs. 0%). Total length of stay was significantly different between the two groups as well (6 days vs. 3 days, p < 0.01). Both groups had a change in LVEF of ~10%. One-year outcomes were similar between the groups. All-cause mortality at 1 year was not significantly different at 1 year (13% for VLEF vs. 11% for LEF), and KCC scores were also similar (77.54 vs. 74.97). Mean aortic valve gradients were also similar (12 mmHg vs. 11 mmHg, p = 0.48). Our study suggests that patients with EF ≤ 20% can safely have TAVR with similar periprocedural outcomes compared to patients with EF > 20% to ≤40% despite higher rates of vasopressor and mechanical support.
Treatment of Biowaste for Electrodes in Energy Storage Applications: A Brief Review
Authors: Kayode Samuel Ebenezer , González Francisco J.
Published: March 2023

Proper and innovative waste management methods still pose a major concern in our present world. Continuous accumulation of biowaste from bio-processing industries, household, organic residues and so on makes the environment polluted and endangers the health of man and other animals. The common waste management methods which include direct dumping into water bodies, open-air combustion, and as land fillers are obsolete and are the major causes of environmental pollution. Conversion of biowastes into valuable materials aids proper waste management, and helps to attain a cleaner environment, in addition to the fact that wastes are turned into wealth. Biowastes are rich in carbon and can serve as excellent precursors for the synthesis of important carbon materials such as activated carbon, graphene, carbon nanotubes etc. Three important methods of converting biowastes into carbon materials are discussed in this review. The electrochemical, adsorption, and electrocatalytic properties of the materials and the applications in electrochemical energy storage devices are also discussed in brief. This review focuses on the synthesis of carbon materials from biowaste residues and their use in developing electrode materials for batteries and supercapacitors. Future perspectives on the need to exploit greener technology for the conversion of biowastes into important carbon materials should be considered.
On The Potential of Image Moments for Medical Diagnosis
Authors: Di Ruberto Cecilia , Loddo Andrea , Putzu Lorenzo
Published: March 2023

Medical imaging is widely used for diagnosis and postoperative or post-therapy monitoring. The ever-increasing number of images produced has encouraged the introduction of automated methods to assist doctors or pathologists. In recent years, especially after the advent of convolutional neural networks, many researchers have focused on this approach, considering it to be the only method for diagnosis since it can perform a direct classification of images. However, many diagnostic systems still rely on handcrafted features to improve interpretability and limit resource consumption. In this work, we focused our efforts on orthogonal moments, first by providing an overview and taxonomy of their macrocategories and then by analysing their classification performance on very different medical tasks represented by four public benchmark data sets. The results confirmed that convolutional neural networks achieved excellent performance on all tasks. Despite being composed of much fewer features than those extracted by the networks, orthogonal moments proved to be competitive with them, showing comparable and, in some cases, better performance. In addition, Cartesian and harmonic categories provided a very low standard deviation, proving their robustness in medical diagnostic tasks. We strongly believe that the integration of the studied orthogonal moments can lead to more robust and reliable diagnostic systems, considering the performance obtained and the low variation of the results. Finally, since they have been shown to be effective on both magnetic resonance and computed tomography images, they can be easily extended to other imaging techniques.
Phenothiazines Rapidly Induce Laccase Expression and Lignin-Degrading Properties in the White-Rot Fungus Phlebia radiata
Authors: Hirakawa Matthew P. , Rodriguez Alberto , Tran-Gyamfi Mary B. , Light Yooli K. , Martinez Salvador , Diamond-Pott Henry , Simmons Blake A. , Sale Kenneth L.
Published: March 2023

Phlebia radiata is a widespread white-rot basidiomycete fungus with significance in diverse biotechnological applications due to its ability to degrade aromatic compounds, xenobiotics, and lignin using an assortment of oxidative enzymes including laccase. In this work, a chemical screen with 480 conditions was conducted to identify chemical inducers of laccase expression in P. radiata. Among the chemicals tested, phenothiazines were observed to induce laccase activity in P. radiata, with promethazine being the strongest laccase inducer of the phenothiazine-derived compounds examined. Secretomes produced by promethazine-treated P. radiata exhibited increased laccase protein abundance, increased enzymatic activity, and an enhanced ability to degrade phenolic model lignin compounds. Transcriptomics analyses revealed that promethazine rapidly induced the expression of genes encoding lignin-degrading enzymes, including laccase and various oxidoreductases, showing that the increased laccase activity was due to increased laccase gene expression. Finally, the generality of promethazine as an inducer of laccases in fungi was demonstrated by showing that promethazine treatment also increased laccase activity in other relevant fungal species with known lignin conversion capabilities including Trametes versicolor and Pleurotus ostreatus.
High Andean Steppes of Southern Chile Contain Little-Explored Peltigera Lichen Symbionts
Authors: Veas-Mattheos Karla , Almendras Katerin , Pezoa Matías , Muster Cecilia , Orlando Julieta
Published: March 2023

Peltigera lichens can colonize extreme habitats, such as high-elevation ecosystems, but their biodiversity is still largely unknown in these environments, especially in the southern hemi- sphere. We assessed the genetic diversity of mycobionts and cyanobionts of 60 Peltigera lichens collected in three high Andean steppes of southern Chile using LSU, β-tubulin, COR3 and ITS loci for mycobionts, and SSU and rbcLX loci for cyanobionts. We obtained 240 sequences for the different mycobiont markers and 118 for the cyanobiont markers, including the first report of β-tubulin sequences of P. patagonica through modifying a previously designed primer. Phylogenetic analyses, ITS scrutiny and variability of haplotypes were used to compare the sequences with those previously reported. We found seven mycobiont species and eleven cyanobiont haplotypes, including considerable novel symbionts. This was reflected by ~30% of mycobionts and ~20% of cyanobionts haplotypes that yielded less than 99% BLASTn sequence identity, 15 new sequences of the ITS1-HR, and a putative new Peltigera species associated with 3 Nostoc haplotypes not previously reported. Our results suggest that high Andean steppe ecosystems are habitats of unknown or little-explored lichen species and thus valuable environments to enhance our understanding of global Peltigera biodiversity.
Cytochalasans from the Endophytic Fungus Aspergillus sp. LE2: Their Structures, Antibacterial and NO Production Inhibitory Activities
Authors: Li Yong-Chao , Yang Jing , Zhao Yuan-Zeng , Ma Qin-Di , Liu Ben-Guo , Sun Jun-Liang
Published: March 2023

Six new cytochalasans—namely, aspergicytochalasins A–F (1–6)—together with five known analogs were isolated and characterized from the endophytic fungus Aspergillus sp. from the medicinal plant Lonicera japonica. The structures of the new compounds were established by NMR and MS methods as well as single crystal X-ray diffractions. Compounds 3 and 4 showed weak antibacterial activities to Staphylococcus aureus, with MIC values of 128 and 64 μg/mL, respectively. Compounds 1, 3, 5 and 6 showed inhibitory activities on NO production, with IC50 values less than 40 μM.
Heterologous Expression, Purification and Characterization of an Alkalic Thermophilic β-Mannanase CcMan5C from Coprinopsis cinerea
Authors: Yan Songling , Duan Baiyun , Liu Cuicui , Liu Guiyou , Kang Liqin , Sun Lei , Yi Lin , Zhang Zhenqing , Liu Zhonghua , Yuan Sheng
Published: March 2023

A endo-1,4-β-mannanase (CcMan5C) gene was cloned from Coprinopsis cinerea and heterologously expressed in Pichia pastoris, and the recombinant enzyme was purified by Ni-affinity chromatography and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). CcMan5C hydrolyzed only locust bean gum galactomannan (LBG) but not α-mannan from S. cerevisiae or Avicel cellulose, oat spelt xylan, or laminarin from Laminaria digitata. CcMan5C exhibited distinctive catalytic features that were different from previously reported β-mannanases. (1) CcMan5C is the first reported fungal β-mannase with an optimal alkalic pH of 8.0–9.0 for hydrolytic activity under assay conditions. (2) CcMan5C is the first reported alkalic fungal β-mannase with an optimal temperature of 70 °C for hydrolytic activity under assay conditions. (3) The organic solvents methanol, ethanol, isopropanol, and acetone at concentrations of 10% or 20% did not inhibit CcMan5C activity, while 10% or 20% isopropanol and acetone even enhanced CcMan5C activity by 9.20–34.98%. Furthermore, CcMan5C tolerated detergents such as Tween 20 and Triton X-100, and its activity was even enhanced to 26.2–45.6% by 1% or 10% Tween 20 and Triton X-100. (4) CcMan5C solution or lyophilized CcMan5C exhibited unchanged activity and even increasing activity after being stored at −20 °C or −80 °C for 12 months and retained above 50% activity after being stored at 4 °C for 12 months. These features make CcMan5C a suitable candidate for the detergent industry and paper and pulp industry.