bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanism of the Intermediary Phase Formation in Ti-20 wt. % Al Mixture during Pressureless Reactive Sintering
Authors: Školáková Andrea , Salvetr Pavel , Novák Pavel , Leitner Jindřich , Deduytsche Davy
Published: July 2019

This work aims to describe the mechanism of intermediary phases formation in TiAl20 (wt. %) alloy composition during reactive sintering. The reaction between titanium and aluminum powders was studied by in situ diffraction and the results were confirmed by annealing at various temperatures. It was found that the Ti2Al5 phase formed preferentially and its formation was detected at 400 °C. So far, this phase has never been found in this alloy composition during reactive sintering processes. Subsequently, the Ti2Al5 phase reacted with the titanium, and the formation of the major phase, Ti3Al, was accompanied by the minor phase, TiAl. Equations of the proposed reactions are presented in this paper and their thermodynamic and kinetic feasibility are supported by Gibbs energies of reaction and reaction enthalpies.
Microstructure and Flux Pinning of Reacted-and-Pressed, Polycrystalline Ba0.6K0.4Fe2As2 Powders
Authors: Koblischka Michael R. , Koblischka-Veneva Anjela , Schmauch Jörg , Murakami Masato
Published: July 2019

The flux pinning properties of reacted-and-pressed Ba0.6K0.4Fe2As2 powder were measured using magnetic hysteresis loops in the temperature range 20 K ≤ T ≤ 35 K. The scaling analysis of the flux pinning forces ( F p = j c × B , with j c denoting the critical current density) following the Dew-Hughes model reveals a dominant flux pinning provided by normal-conducting point defects ( δ l -pinning) with only small irreversibility fields, H irr , ranging between 0.5 T (35 K) and 16 T (20 K). Kramer plots demonstrate a linear behavior above an applied field of 0.6 T. The samples were further characterized by electron backscatter diffraction (EBSD) analysis to elucidate the origin of the flux pinning. We compare our data with results of Weiss et al. (bulks) and Yao et al. (tapes), revealing that the dominant flux pinning in the samples for applications is provided mainly by grain boundary pinning, created by the densification procedures and the mechanical deformation applied.
Ru-Doped Wells–Dawson Polyoxometalate as Efficient Catalyst for Glycerol Hydrogenolysis to Propanediols
Authors: Modvig Amalie , Kumpidet Chiraphat , Riisager Anders , Albert Jakob
Published: July 2019

A Ru-doped phospho-tungstic Wells–Dawson polyoxometalate (POM) was successfully applied as homogeneous catalyst for glycerol hydrogenolysis in aqueous media. The synthesized compound showed superior catalytic activity compared to classical homogeneous/heterogeneous Ru catalysts like RuCl3 and Ru/C under identical reaction conditions, whereas the analogous POM doped with Pd or Pt proved far less activity. Detailed characterization of the POMs was performed using 31P-NMR to identify characteristic phosphorous peaks of the heteroatoms, infrared spectroscopy (ATR-FTIR) to confirm characteristic P-O and W-O-W vibrations, powder XRD for comparison of crystal structures, and X-ray fluorescence (XRF) and inductive-coupled plasma (ICP) analysis to determine elemental composition. Variation of the reaction parameters for the best performing Ru-doped POM catalyst showed that substrate concentration played an important role for both product selectivity and conversion. Moreover, medium hydrogen pressure and high stirring speed were key factors to obtain highly selective conversion of glycerol to 1,2-propanediol.
Numerical Investigation of Polymer Coated Nanoporous Gold
Authors: Gnegel Stephan , Li Jie , Mameka Nadiia , Huber Norbert , Düster Alexander
Published: July 2019

Nanoporous metals represent a fascinating class of materials. They consist of a bi-continuous three-dimensional network of randomly intersecting pores and ligaments where the ligaments form the skeleton of the structure. The open-pore structure allows for applying a thin electrolytic coating on the ligaments. In this paper, we will investigate the stiffening effect of a polymer coating numerically. Since the coating adds an additional difficulty for the discretization of the microstructure by finite elements, we apply the finite cell method. This allows for deriving a mesh in a fully automatic fashion from the high resolution 3D voxel model stemming from the 3D focused ion beam-scanning electron microscope tomography data of nanoporous gold. By manipulating the voxel model in a straightforward way, we add a thin polymer layer of homogeneous thickness numerically and study its effect on the macroscopic elastic properties systematically. In order to lower the influence of the boundary conditions on the results, the window method, which is known from homogenization procedures, is applied. In the second part of the paper, we fill the gap between numerical simulations and experimental investigations and determine real material properties of an electrolytic applied polypyrrole coating by inverse computations. The simulations provide an estimate for the mechanical properties of the ligaments and the polymeric coating and are in accordance with experimental data.
Graphene Oxide and Its Inorganic Composites: Fabrication and Electrorheological Response
Authors: Dong Yu Zhen , Kim Joo Nyeon , Choi Hyoung Jin
Published: July 2019

Composite particles associated with graphene oxide (GO) and inorganic materials provide the synergistic properties of an appropriate electrical conductivity of GO with the good dielectric characteristics of inorganic materials, making them attractive candidates for electrorheological (ER) materials. This review paper focuses on the fabrication mechanisms of GO/inorganic composites and their ER response when suspended in a non-conducting medium, including steady shear flow curves, dynamic yield stress, On-Off tests, and dynamic oscillation analysis. Furthermore, the morphologies of these composites, dielectric properties, and sedimentation of the ER fluids are covered.
Hot Deformation Behavior of Q345 Steel and Its Application in Rapid Shear Connection
Authors: Wu Mengwei , Zhang Shuangjie , Ma Shibo , Yan Huajun , Wang Wei , Li Qiang
Published: July 2019

The high-temperature deformation behavior of Q345 steel is detected by a Gleeble-3800 thermal simulator. The Arrhenius constitutive equation for high-temperature flow stress and the dynamic recrystallization model are constructed. With the secondary development technology, customized modifications are made on existing Deform-3D software. The constructed constitutive model and dynamic recrystallization model are embedded into Deform-3D to realize the secondary development of Deform-3D. The grain size and volume percentage distribution of dynamic recrystallization are obtained by simulating the shear connection process at high temperature and high speed. The results show that the constitutive equation and the dynamic recrystallization model constructed in this paper can be used to predict the evolution of the microstructure. The difference between the prediction results and the experimental data is about 3%. The accuracy of Arrhenius constitutive equation, dynamic recrystallization model and the feasibility of software secondary development are verified.
Graphene Quantum Dots as Nanozymes for Electrochemical Sensing of Yersinia enterocolitica in Milk and Human Serum
Authors: Savas Sumeyra , Altintas Zeynep
Published: July 2019

The genus Yersinia contains three well-recognized human pathogens, including Y. enterocolitica, Y. pestis, and Y. pseudotuberculosis. Various domesticated and wild animals carry Yersinia in their intestines. Spread to individuals arises from eating food or water contaminated by infected human or animal faeces. Interaction with infected pets and domestic stock may also lead to infection. Yersinia is able to multiply at temperatures found in normal refrigerators; hence, a large number of the bacteria may be present if meat is kept without freezing. Yersinia is also rarely transmitted by blood transfusion, because it is able to multiply in stored blood products. Infection with Yersinia can cause yersiniosis, a serious bacterial infection associated with fever, abdominal pain and cramps, diarrhea, joint pain, and symptoms similar to appendicitis in older children and adults. This paper describes a novel immunosensor approach using graphene quantum dots (GQDs) as enzyme mimics in an electrochemical sensor set up to provide an efficient diagnostic method for Y. enterecolitica. The optimum assay conditions were initially determined and the developed immunosensor was subsequently used for the detection of the bacterium in milk and human serum. The GQD-immunosensor enabled the quantification of Y. enterocolitica in a wide concentration range with a high sensitivity (LODmilk = 5 cfu mL−1 and LODserum = 30 cfu mL−1) and specificity. The developed method can be used for any pathogenic bacteria detection for clinical and food samples without pre-sample treatment. Offering a very rapid, specific and sensitive detection with a label-free system, the GQD-based immunosensor can be coupled with many electrochemical biosensors.
Effects of Hot Sterilization on Torsional Properties of Endodontic Instruments: Systematic Review with Meta-Analysis
Authors: Dioguardi Mario , Sovereto Diego , Aiuto Riccardo , Laino Luigi , Illuzzi Gaetano , Laneve Enrica , Raddato Bruna , Caponio Vito Carlo Alberto , Dioguardi Antonio , Zhurakivska Khrystyna , Troiano Giuseppe , Lo Muzio Lorenzo
Published: July 2019

Sterilization is a fundamental step in the reuse of endodontic instruments. The sterilization procedure involves disinfection, cleaning, washing, drying, packaging, and sterilization by heat. Heat sterilization can lead to changes in the physical and mechanical properties of dental instruments. These changes can affect the external surfaces via micropitting, corrosion, a reduction in cutting capacity, and/or an influence on the resistance to cyclic fatigue or to torsional fatigue. In this study, we examined the modification of the torsional properties of endodontic instruments after hot sterilization, and compared the properties with instruments not subjected to hot sterilization cycles in terms of resistance to torsional fatigue and deflection angle in NiTi and steel instruments. The following work was performed based on the PRISMA indications. Studies were identified through bibliographic research using electronic databases. A total of 725 records were identified in the PubMed and Scopus databases. A total of 685 records remained after exclusion by year of publication (1979 to 2019). With the application of the eligibility criteria (all articles pertaining to the issue of sterilization in endodontics), we found 146 articles, which decreased to 130 articles after elimination of duplications. There were 45 articles that studied the influences of sterilization procedures on the physical and mechanical characteristics of the instruments, and 12 that measured parameters related to resistance to torsional fatigue. Applying the inclusion and exclusion criteria resulted in a total of eight articles for quantitative analysis. The meta-analysis results show a pejorative effect of torsional fatigue for NiTi instruments subjected to heat sterilization compared to the non-sterilized control.
Field Test of Self-Cleaning Zr-Modified-TiO2-SiO2 Films on Glass with a Demonstration of Their Anti-Fogging Effect
Authors: Šuligoj Andraž , Pliekhova Olena , Vodišek Nives , Mihelčič Mohor , Surca Angelja , Kunič Roman , Šubic Barbara , Starman Jernej , Ugovšek Aleš , Lavrenčič Štangar Urška
Published: July 2019

The number of commercial products claiming self-cleaning properties is rising and testing of long-term activity and durability of such coatings needs to be addressed more. The time-dependent changes of different characteristics like haze, transparency, and color are essential for transparent glazing materials. Herein, we aimed to examine whether the laboratory results obtained on the Zr-modified-titania-silica (TiZr) self-cleaning materials would translate to larger-scale outdoor-exposed testing. TiZr thin films were deposited via spraying onto float glass window surfaces and exposed into three different environments for 20 months. For comparison, a commercially available active SGG BIOCLEANTM glass and standard float glass were simultaneously exposed in the same conditions. It was shown that the self-cleaning property of either a commercial product or TiZr-coated float glass was not considerably effective in real field test conditions, although the previous laboratory tests showed pronounced photocatalytic activity of TiZr thin films. The inclination angle; however, was shown to have a considerable effect on the self-cleaning ability of samples, as did the rain patterns during the testing period. On the other hand, the anti-fogging effect of our TiZr material was very well expressed in controlled laboratory conditions (measuring droplet formation time) as well as in the real outdoor environment.
Friction Behavior of Silver Perrhenate in Oil as Lubricating Additive for Use at Elevated Temperatures
Authors: Wang Junhai , Li Ting , Yan Tingting , Wei Xiaoyi , Qu Xin , Yuan Shuai
Published: July 2019

In this study, we use an aqueous solution synthesis method to prepare silver perrhenate powders and suspend them into a poly alpha olefin (PAO) base oil with polyoxyethylene octylphenyl ether. Four ball tests and ball-on-disk reciprocating mode are performed to determine how silver perrhenate performs tribologically as a lubricating additive over a wide range of temperatures. The physical and chemical properties, as well as the lubricating mechanisms of the silver perrhenate additive, are characterized via X-ray diffraction, scanning electron microscope, Fourier transformation infrared spectroscopy, Raman spectrum, and X-ray photoelectron spectroscopy. The four-ball test results demonstrate that the oil added with silver perrhenate additive is more effective than the base oil in reducing friction and improving wear resistance, and provides the best lubricating performance when at a concentration of 0.5 wt%. The reciprocating mode findings indicate that the hybrid lubricant exhibits distinctively better tribological properties than the base oil at high temperatures, and its low shear strength and chemical inertness allow for low friction at elevated temperatures. The resulting silver perrhenate layer that incorporates native superalloy oxides on the worn surface can provide lubrication by serving as a barrier that prevents direct contact between the rubbing surfaces at elevated temperatures.
Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway
Authors: Zhang Zhuangwei , Hu Xuyang , Lin Lin , Ding Guofang , Yu Fangmiao
Published: July 2019

In this study, a low molecular-weight (Mw) peptide named NJP (<1 kDa), was purified from a protein hydrolysate of Nibea japonica by ultrafiltration, and its immunomodulatory effect on RAW264.7 cells was evaluated. The lactate dehydrogenase (LDH) and MTT assays were performed to explore the cytotoxicity of NJP. The results showed that NJP promoted cell proliferation and had no significant toxic effects on RAW264.7 cells. Moreover, the cells formed multiple pseudopodia indicating that they were in activated state. Further tests showed that NJP significantly promoted phagocytic capacity, and the secretion of proinflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). It also increased the synthesis of nitric oxide (NO) by upregulating inducible nitric oxide synthase (iNOS) protein level. Flow cytometry revealed that NJP promoted cell cycle progression and increased the percentage of cells in G0/G1 phase. NJP promoted IκBα degradation, p65 and nuclear factor (NF)-κB activation and translocation by up-regulating IKKα/β protein expression. In conclusion, these results indicated that NJP exerts immunomodulatory effects on RAW264.7 cells through the NF-κB signaling pathway. Therefore, NJP can be incorporated in the production of functional foods or nutraceuticals.
Congenital Sensorineural Hearing Loss and Inborn Pigmentary Disorders: First Report of Multilocus Syndrome in Piebaldism
Authors: Gironi Laura Cristina , Colombo Enrico , Brusco Alfredo , Grosso Enrico , Naretto Valeria Giorgia , Guala Andrea , Di Gregorio Eleonora , Zonta Andrea , Zottarelli Francesca , Pasini Barbara , Savoia Paola
Published: July 2019

Congenital sensorineural hearing loss may occur in association with inborn pigmentary defects of the iris, hair, and skin. These conditions, named auditory-pigmentary disorders (APDs), represent extremely heterogeneous hereditary diseases, including Waardenburg syndromes, oculocutaneous albinism, Tietz syndrome, and piebaldism. APDs are part of the neurocristopathies, a group of congenital multisystem disorders caused by an altered development of the neural crest cells, multipotent progenitors of a wide variety of different lineages, including those differentiating into peripheral nervous system glial cells and melanocytes. We report on clinical and genetic findings of two monozygotic twins from a large Albanian family who showed a complex phenotype featured by sensorineural congenital deafness, severe neuropsychiatric impairment, and inborn pigmentary defects of hair and skin. The genetic analyzes identified, in both probands, an unreported co-occurrence of a new heterozygous germline pathogenic variant (c.2484 + 5G > T splicing mutation) in the KIT gene, consistent with the diagnosis of piebaldism, and a heterozygous deletion at chromosome 15q13.3, responsible for the neuropsychiatric impairment. This case represents the first worldwide report of dual locus inherited syndrome in piebald patients affected by a complex auditory-pigmentary multisystem phenotype. Here we also synthesize the clinical and genetic findings of all known neurocristopathies characterized by a hypopigmentary congenital disorder.
Comparison of Physiological and Biochemical Autonomic Indices in Children with and without Autism Spectrum Disorders
Authors: Bharath Remya , Moodithaya Shailaja S. , Bhat Shrinivasa U. , Mirajkar Amrit M. , Shetty Sumanth B.
Published: July 2019

Background and objectives: Autism Spectrum Disorder (ASD) is a complex neuro-developmental disorder and it has been suggested that symptoms of ASD are associated with neural networks that regulate the Autonomic Nervous System (ANS). However, the nature of autonomic atypicalities in ASDs remain largely unknown. Measures like Heart Rate Variability (HRV) and urinary Vanillylmandelic Acid (VMA) estimation are sensitive and non-invasive physiological and biochemical indicators of autonomic nervous activity. This study aimed to compare the physiological and biochemical autonomic indices in children with and without ASD. Materials and Methods: In this case-control study, 40 children with autism and 40 Typically Developing (TD) children were recruited. Measures of physiological autonomic index were assessed by the analysis of short term HRV, and the urinary levels of VMA estimation was used as a biochemical autonomic index. Results: Cardiac sympathetic activity assessed by Low Frequency (nu) of HRV was significantly higher in the ASD group in comparison with the TD group (p = 0.006). On the contrary, both the High Frequency (abs) and (nu) of HRV were found to be significantly lower in autistic children (p = 0.034 and p = 0.000) than controls. Autistic children also exhibited a significantly higher level (p = 0.049) of VMA concentration compared to TD children. Conclusion: The study concludes that children with ASD exhibit lower cardio-vagal activity as measured by HRV and increased sympathetic activity as assessed by urinary VMA compared to that of TD children. The core autistic symptoms exhibited by children with ASD could be due to the differences in baseline arousal or stress which might be associated with autonomic dysfunction. Further studies are needed to examine the association of this autonomic dysregulation with ASD symptoms and comorbidities.
Deformation Behavior of a Double Soaked Medium Manganese Steel with Varied Martensite Strength
Authors: Glover Alexandra , Gibbs Paul J. , Liu Cheng , Brown Donald W. , Clausen Bjørn , Speer John G. , De Moor Emmanuel
Published: July 2019

The effects of athermal martensite on yielding behavior and strain partitioning during deformation is explored using in situ neutron diffraction for a 0.14C–7.14Mn medium manganese steel. Utilizing a novel heat treatment, termed double soaking, samples with similar microstructural composition and varied athermal martensite strength and microstructural characteristics, which composed the bulk of the matrix phase, were characterized. It was found that the addition of either as-quenched or tempered athermal martensite led to an improvement in mechanical properties as compared to a ferrite plus austenite medium manganese steel, although the yielding and work hardening behavior were highly dependent upon the martensite characteristics. Specifically, athermal martensite was found to promote continuous yielding and improve the work hardening rate during deformation. The results of this study are particularly relevant when considering the effect of post-processing thermal heat treatments, such as tempering or elevated temperature service environments, on the mechanical properties of medium manganese steels containing athermal martensite.
Interface Structure and Mechanical Properties of 7075Al Hybrid Composite Reinforced with Micron Ti Metal Particles Using Pressure Infiltration
Authors: Liu Yixiong , Zheng Zhenxing , Cao Genghua , Zhu Dezhi , Yang Chao , Luo Mingqiang
Published: July 2019

Micron Ti metal particles were incorporated into SiCp/7075Al composites using pressure infiltration. The interface structure between the Ti metal particles and the matrix during the casting processes were investigated. Results show that the dispersed unreacted Ti particles form mutual diffusion layer at the interface without the formation of low-temperature intermetallic phases during the solidification processes. The interaction between the micron Ti and the molten aluminum alloy is subject to the mutual diffusion coefficient of Ti–Al rather than the reaction activation energy. The tensile strength and plasticity of the composite were improved simultaneously due to the high interfacial bonding strength and released thermal misfit stress cause by the incorporated Ti metal particles.
Editorial for the Special Issue on Optical MEMS
Authors: Xie Huikai , Zamkotsian Frederic
Published: July 2019

Optical micro-electro-mechanical systems (MEMS), micro-opto-electro-mechanical systems (MOEMS), or optical microsystems are devices or systems that interact with light through actuation or sensing at a micron or millimeter scale [...]
Stable Deuterium Labeling of Histidine-Rich Lysine-Based Dendrimers
Authors: Sheveleva Nadezhda N. , Markelov Denis A. , Vovk Mikhail A. , Tarasenko Irina I. , Mikhailova Mariya E. , Ilyash Maxim Yu , Neelov Igor M. , Lahderanta Erkki
Published: July 2019

Peptide dendrimers, due to their biocompatibility and low toxicity, are highly promising candidates as nanocarriers for drugs and genes. The development of this kind of delivery system requires reliable monitoring of their metabolic and biological pathways. In this respect, hydrogen isotope labeling has tremendous importance, being a safe tool for detection of the labeled nanocarriers. In this work, we have synthesized new histidine-rich lysine-based dendrimers (Lys-2His dendrimer) with two linear histidine (His) residues in every inner segment. The presence of His residues has enabled us to perform controlled deuteration of Lys-2His dendrimers. The high deuteration degree (around 70%) does not practically change after redissolving the samples in H2O and heating them at 40 °C, which indicates the isotopic label stability.
Comparison of the Aroma Profiles of Intermediate Wheatgrass and Wheat Bread Crusts
Authors: Paravisini Laurianne , Sneddon Kelsey A. , Peterson Devin G.
Published: July 2019

The aroma profiles of bread crusts made from intermediate wheatgrass (Thinopyrum intermedium) and whole wheat (Triticum aestivum) flours were compared. Based on gas chromatography/mass spectrometry/olfactometry analysis, twenty-four odorants were identified and further quantified. The concentrations of seventeen compounds were significantly different between intermediate wheatgrass and whole wheat bread crusts, of which sixteen compounds were higher in the whole wheat sample. The aroma profiles of the bread samples were subsequently characterized using sensory descriptive analysis (DA) and indicated that the roasted attribute was perceived at a significantly higher intensity in the whole wheat sample due to a greater amount of Maillard reaction compounds. Alternatively, bran and green notes were perceived at higher intensities in the intermediate wheatgrass sample, however they were not attributed to the presence of specific compounds but rather to a change in the aroma composition. Aroma recombination DA of the whole wheat and intermediate wheatgrass aroma models was similar to the original aroma profiles of the bread samples, demonstrating the sensory relevance of the identified odorants.
Volatile Aroma Compounds of Brandy ‘Lozovača′ Produced from Muscat Table Grapevine Cultivars (Vitis vinifera L.)
Authors: Matijašević Saša , Popović-Djordjević Jelena , Ristić Renata , Ćirković Dušica , Ćirković Bratislav , Popović Tatjana
Published: July 2019

Grape brandy, known as ‘Lozovača’, is one of the most produced alcoholic beverages in the Republic of Serbia. Muscat cultivars are highly priced in grape brandy manufacturing. Among the numerous factors, cultivar-specific characteristics have a significant influence on its quality and aroma profile. Pectolytic enzymes play a part in increasing intensity of the prefermentative aroma by hydrolysis of terpenic glycosides, from which the compounds that contribute to the aroma of brandy are released. In this study, grape brandy samples were produced from five Muscat table grapevine cultivars (Vitis vinifera L.) namely, Early Muscat, Radmilovac Muscat, Banat Muscat, Italia Muscat, and Muscat Hamburg, with the addition of pectolytic enzyme in two different concentrations or without it (control). A total of 58 volatile aroma compounds were detected by means of combined gas chromatographic-mass spectrometric (GC/MS) method. Ethyl esters of C8–C18 fatty acids (21) and terpene (16) compounds were considerably more abundant in all grape brandy samples compared to the other volatile compounds identified. Pectolytic enzyme, positively affected terpenes content in the brandy of all studied cultivars. The similarities between brandy samples produced from Muscat Hamburg (MH) and other Muscat cultivars may be attributed to the parentage of MH to those cultivars.
New 11,20-Epoxybriaranes from the Gorgonian Coral Junceella fragilis (Ellisellidae)
Authors: Lin Chia-Cheng , Su Jui-Hsin , Chen Wu-Fu , Wen Zhi-Hong , Peng Bo-Rong , Huang Lin-Cyuan , Hwang Tsong-Long , Sung Ping-Jyun
Published: July 2019

Two new 11,20-epoxybriaranes, fragilides P (1) and Q (2), as well as two known analogues, robustolide F (3) and juncin Z (4), were obtained from the gorgonian coral Junceella fragilis. The structures, including the absolute configurations of briaranes 1 and 2, were elucidated by using spectroscopic methods and comparing the spectroscopic and rotation data with those of known related analogues. Briarane 4 decreased the generation of superoxide anions by human neutrophils. The propionate group in 1 is rarely found.
Evaluation of Glycosyl-Hydrolases, Phosphatases, Esterases and Proteases as Potential Biomarker for NaCl-Stress Tolerance in Solanum lycopersicum L. Varieties
Authors: Reyes-Pérez Juan José , Ruiz-Espinoza Francisco Higinio , Hernández-Montiel Luis Guillermo , de Lucía Barbara , Cristiano Giuseppe , Murillo-Amador Bernardo
Published: July 2019

Salinity stress limited the production in over 30% of irrigated crops and 7% of dryland agriculture worldwide. The objective was to evaluate the effects of NaCl-stress on the enzymatic activity in tomato. Two experiments were carried out in germination and early vegetative growth stages. The activity of proline and peroxidase of eight varieties (Missouri, Yaqui, Vita, Feroz, Rio Grande, Tropic, Ace, and Floradade) submitted to NaCl concentrations (0, 50, 100, 150 and 200 mM de NaCl) and the semi-quantitative activity of 19 enzymes APY ZYM® were measured under a completely randomized design with four replications. Data were analyzed using univariate-multivariate analysis of variance, Tukey’s HSD (p = 0.05), canonical discriminant and cluster analysis. The results showed significant differences between varieties and NaCl in proline content. Proline increased as the NaCl concentration increased. Peroxidase did no show significant differences. Eight enzymes were included within the model to properly classify the varieties and NaCl. In shoots, varieties and NaCl showed that enzymatic activity was higher in the order of alkaline-phosphatase > leucine arylamidase > acid phosphatase > naphthol-AS-BI-phosphohydrolase > n-acetyl-β-glucosaminidase > β-galactosidase, while in roots was higher in the order of alkaline-phosphatase > naphthol-AS-BI-phosphohydrolase > acid phosphatase > n-acetyl-β-glucosaminidase. Acid and alkali phosphatase, lipase, esterase, β-galactosidase, and trypsin can be a potential biomarker for NaCl-stress tolerance in tomato.
Development of Novel antimiRzymes for Targeted Inhibition of miR-21 Expression in Solid Cancer Cells
Authors: Larcher Leon M. , Wang Tao , Veedu Rakesh N.
Published: July 2019

MicroRNAs (miRNAs) are short non-coding RNAs that are involved in the regulation of gene expression. Previous reports showed an over-expression of miRNA-21 (miR-21) in various cancer cells, and its up-regulation is closely related to cancer initiation, proliferation and metastasis. In this work, we envisioned the development of novel antimiRzymes (anti-miRNA-DNAzyme) that are capable of selectively targeting and cleaving miR-21 and inhibit its expression in cancer cells using the DNAzyme technique. For this purpose, we have designed different antimiRzyme candidates by systematically targeting different regions of miR-21. Our results demonstrated that RNV541, a potential arm-loop-arm type antimiRzyme, was very efficient (90%) to suppress miR-21 expression in U87MG malignant glioblastoma cell line at 200 nM concentration. In addition, RNV541 also inhibited miR-21 expression (50%) in MDA-MB-231 breast cancer cell line. For targeted delivery, we conjugated RNV541 with a transferrin receptor (TfR) targeting aptamer for TfR-mediated cancer cell delivery. As expected, the developed chimeric structure efficiently delivered the antimiRzyme RNV541 into TfR positive glioblastoma cells. TfR aptamer-RNV541 chimeric construct showed 52% inhibition of miR-21 expression in U87MG glioblastoma cells at 2000 nM concentration, without using any transfection reagents, making it a highly desirable strategy to tackle miR-21 over-expressed malignant cancers. Although these are in vitro based observations, based on our results, we firmly believe that our findings could be beneficial towards the development of targeted cancer therapeutics where conventional therapies face several challenges.
Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels
Authors: Barranger Audrey , Langan Laura M. , Sharma Vikram , Rance Graham A. , Aminot Yann , Weston Nicola J. , Akcha Farida , Moore Michael N. , Arlt Volker M. , Khlobystov Andrei N. , Readman James W. , Jha Awadhesh N.
Published: July 2019

This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by Gas Chromatography–Mass Spectrometry (GC–MS), Liquid Chromatography–High Resolution Mass Spectrometry (LC–HRMS) and Inductively Coupled Plasma Mass Spectrometer (ICP–MS). Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the interactive effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were downregulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR). Using DEPs identified at a threshold of (p < 0.05; B[a]P and B[a]P mixture with nC60), gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that these proteins were enriched with a broad spectrum of biological processes and pathways, including those broadly associated with protein processing, cellular processes and environmental information processing. Among those significantly enriched pathways, the ribosome was consistently the top enriched term irrespective of treatment or concentration and plays an important role as the site of biological protein synthesis and translation. Our results demonstrate the complex multi-modal response to environmental stressors in M. galloprovincialis.
Modulation of Milk Allergenicity by Baking Milk in Foods: A Proteomic Investigation
Authors: Bavaro Simona L. , De Angelis Elisabetta , Barni Simona , Pilolli Rosa , Mori Francesca , Novembre Elio. M. , Monaci Linda
Published: July 2019

Cow’s milk is considered the best wholesome supplement for children since it is highly enriched with micro and macro nutrients. Although the protein fraction is composed of more than 25 proteins, only a few of them are capable of triggering allergic reactions in sensitive consumers. The balance in protein composition plays an important role in the sensitization capacity of cow’s milk, and its modification can increase the immunological response in allergic patients. In particular, the heating treatments in the presence of a food matrix have demonstrated a decrease in the milk allergenicity and this has also proved to play a pivotal role in developing tolerance towards milk. In this paper we investigated the effect of thermal treatment like baking of cow’s milk proteins that were employed as ingredients in the preparation of muffins. A proteomic workflow was applied to the analysis of the protein bands highlighted along the SDS gel followed by western blot analyses with sera of milk allergic children in order to have deeper information on the impact of the heating on the epitopes and consequent IgE recognition. Our results show that incorporating milk in muffins might promote the formation of complex milk–food components and induce a modulation of the immunoreactivity towards milk allergens compared to milk baked in the oven at 180 °C for ten minutes. The interactions between milk proteins and food components during heating proved to play a role in the potential reduction of allergenicity as assessed by in vitro tests. This would help, in perspective, in designing strategies for improving milk tolerance in young patients affected from severe milk allergies.
Seminal but not Serum Levels of Holotranscobalamin are Altered in Morbid Obesity and Correlate with Semen Quality: A Pilot Single Centre Study
Authors: Samavat Jinous , Cantini Giulia , Lorubbio Maria , Degl’Innocenti Selene , Adaikalakoteswari Antonysunil , Facchiano Enrico , Lucchese Marcello , Maggi Mario , Saravanan Ponnusamy , Ognibene Agostino , Luconi Michaela
Published: July 2019

Vitamin B12 (cobalamin) is an essential cofactor in the one-carbon metabolism. One-carbon metabolism is a set of complex biochemical reactions, through which methyl groups are utilised or generated, and thus plays a vital role to many cellular functions in humans. Low levels of cobalamin have been associated to metabolic/reproductive pathologies. However, cobalamin status has never been investigated in morbid obesity in relation with the reduced semen quality. We analysed the cross-sectional data of 47-morbidly-obese and 21 lean men at Careggi University Hospital and evaluated total cobalamin (CBL) and holotranscobalamin (the active form of B12; holoTC) levels in serum and semen. Both seminal and serum concentrations of holoTC and CBL were lower in morbidly obese compared to lean men, although the difference did not reach any statistical significance for serum holoTC. Seminal CBL and holoTC were significantly higher than serum levels in both groups. Significant positive correlations were observed between seminal holoTC and total sperm motility (r = 0.394, p = 0.012), sperm concentration (r = 0.401, p = 0.009), total sperm number (r = 0.343, p = 0.028), and negative correlation with semen pH (r = −0.535, p = 0.0001). ROC analysis supported seminal holoTC as the best predictor of sperm number (AUC = 0.769 ± 0.08, p = 0.006). Our findings suggest that seminal rather than serum levels of holoTC may represent a good marker of semen quality in morbidly obese subjects.
Diversity of Gut Microbiota Affecting Serum Level of Undercarboxylated Osteocalcin in Patients with Crohn’s Disease
Authors: Wagatsuma Kohei , Yamada Satoshi , Ao Misora , Matsuura Minoru , Tsuji Hidemi , Iida Tomoya , Miyamoto Kentaro , Oka Kentaro , Takahashi Motomichi , Tanaka Kiyoshi , Nakase Hiroshi
Published: July 2019

Several reports have indicated a possible link between decreasing plasma levels of vitamin K and bone mineral density. It has been suggested that intestinal bacteria contribute to maintenance of vitamin K. Several factors are involved in the reduction of vitamin K in patients with Crohn’s disease (CD). We aimed to assess the relationship between gut microbiota and alternative indicators of vitamin K deficiency in patients with CD. We collected the feces of 26 patients with clinically inactive CD. We extracted 16S rRNA from the intestinal bacteria in the feces and amplified it by polymerase chain reaction. The generated polymerase chain reaction product was analyzed using a 16S metagenomic approach by Illumina Miseq platform. Serum undercarboxylated osteocalcin concentration was used as an alternative indicator of vitamin K deficiency. There was a significant negative correlation between serum undercarboxylated osteocalcin and mean Chao1 index in cases of low activity. The diversity of the gut microbiota was significantly lower, and Ruminococcaceae and Lachnospiraceae were significantly decreased in the vitamin K-deficient group in comparison to the vitamin K-normal group. Taken together, these data suggested the significance of investigating the gut microbiota even in patients with clinically inactive CD for improving patients’ vitamin K status.
Analysis of the Preheating Phase of Micro-Arc Discharge in Seawater, Operated Using a Needle-to-Plane Electrode with Variation in the Tip Shape
Authors: Gamaleev Vladislav , Hiramatsu Mineo , Ito Masafumi , Furuta Hiroshi , Hatta Akimitsu
Published: July 2019

In this work, micro-arc discharge is investigated using a needle-to-plane electrode system placed with a micro-gap in highly conductive artificial seawater. A major problem with microarc discharge is the erosion of electrodes caused by the high current of the arc; however, it was found that erosion of the needle electrode did not have any effect on the discharge process in the case of precise control of the discharge gap. A simple mathematical model was developed for a more detailed study of the preheating phase of the discharge. The modeling showed good agreement with the experimental results and confirmed that the needle electrode could be reused to generate reproducible micro-arc discharges even after the erosion caused by the arc. Moreover, it was found that, in certain conditions, the preheating phase could be simulated using a simple inductor-capacitor-resistor (LCR) oscillator model with a resistor instead of electrodes immersed in the liquid. It was confirmed that the shape of the needle electrode’s tip did not affect the measurement of optical emission spectra in the case of precise focusing, which could be used in the development of compact analytical tools for on-site analysis of deep-sea water using atomic emission spectroscopy.
Designing Cellular Structures for Additive Manufacturing Using Voronoi–Monte Carlo Approach
Authors: Liu Tao , Guessasma Sofiane , Zhu Jihong , Zhang Weihong
Published: July 2019

This study aims at reporting a strategy of designing cellular materials based on Voronoi–Monte Carlo approach for additive manufacturing. The approach is implemented to produce a fully connected cellular structure in the design space without producing material discontinuity. The main characteristics of the cellular structure, such as the density and the cell size, are controlled by means of two generation parameters, namely the number of seed points and the relaxation time. The generated cellular structures representing various designs of generated cellular wrenches are converted into surface tessellations and manufactured using stereolithography. Bending experiments are performed up to the rupture point and main attributes representing the performance of the SL-based cellular wrenches are studied with respect to the generation parameters. The results show only slight difference between CAD (Computer-Aided Design) models of the design and the real printed parts. The number of seed points is found to control the main feature of the wrench performance whereas the relaxation time is found to have a secondary effect.
Deformation Mechanisms of Twinning-Induced Plasticity Steel under Shock-Load: Investigated by Synchrotron X-ray Diffraction
Authors: Yan Kun , Callaghan Mark D. , Liss Klaus-Dieter
Published: July 2019

As an ideal candidate material for automobiles, twinning-induced plasticity (TWIP) steels possess excellent formability, high strength and high energy absorption ability during collision. This is attributed to its deformation mechanism of mechanical twinning, resulting in a high work hardening rate. In the current study, deformation mechanisms of low-stacking fault energy TWIP steel, under different strain rates between 0.01/s to 1581/s, were investigated by high-energy X-ray diffraction. After compression, grains with {110}||compression direction became favourable. Higher intensity was observed near brass and A components in the selected orientation distribution function (ODF) section (φ2 = 45°) for all the compressed specimens. The activity of twinning was found to be the highest in the specimens that had been compressed with medium–high strain rates (e.g., 100/s and 10/s), while the texture component related to slip had stronger intensity in the specimen deformed with a quasi-static strain rate (0.01/s).
Evaluation of the Snow Albedo Retrieved from the Snow Kernel Improved the Ross-Roujean BRDF Model
Authors: Ding Anxin , Jiao Ziti , Dong Yadong , Zhang Xiaoning , Peltoniemi Jouni I. , Mei Linlu , Guo Jing , Yin Siyang , Cui Lei , Chang Yaxuan , Xie Rui
Published: July 2019

The original kernel-driven bidirectional reflectance distribution function (BRDF) models were developed based on soil-vegetation systems. To further improve the ability of the models to characterize the snow surface scattering properties, a snow kernel was derived from the asymptotic radiative transfer (ART) model and used in the kernel-driven BRDF model framework. However, there is a need to further evaluate the influence of using this snow kernel to improve the original kernel-driven models in snow albedo retrieval applications. The aim of this study is to perform such an evaluation using a variety of snow BRDF data. The RossThick-Roujean (RTR) model is used as a framework for taking in the new snow kernel (hereafter named the RTS model) since the Roujean geometric-optical (GO) kernel captures a neglectable hotspot effect and represents a more prominent dome-shaped BRDF, especially at a small solar zenith angle (SZA). We obtained the following results: (1) The RTR model has difficulties in reconstructing the snow BRDF shape, especially at large SZAs, which tends to underestimate the reflectance in the forward direction and overestimate reflectance in the backward direction for various data sources. In comparison, the RTS model performs very well in fitting snow BRDF data and shows high accuracy for all data. (2) The RTR model retrieved snow albedos at SZAs = 30°–70° are underestimated by 0.71% and 0.69% in the red and near-infrared (NIR) bands, respectively, compared with the simulation results of the bicontinuous photon tracking (bic-PT) model, which serve as “real” values. However, the albedo retrieved by the RTS model is significantly improved and generally agrees well with the simulation results of the bic-PT model, although the improved model still somewhat underestimates the albedo by 0.01% in the red band and overestimates the albedo by 0.05% in the NIR band, respectively, at SZAs = 30°–70°, which may be negligible. (3) The albedo derived by these two models shows a high correlation (R2 > 0.9) between the field-measured and Polarization and Directionality of the Earth’s Reflectances (POLDER) data, especially for the black-sky albedo. However, the albedo derived using the RTR model is significantly underestimated compared with the RTS model. The RTR model underestimates the black-sky albedo (white-sky albedo) retrievals by 0.62% (1.51%) and 0.93% (2.08%) in the red and NIR bands, respectively, for the field-measured data. The shortwave black-sky and white-sky albedos derived using the RTR model for the POLDER data are underestimated by 1.43% and 1.54%, respectively, compared with the RTS model. These results indicate that the snow kernel in the kernel-driven BRDF model frame is more accurate in snow albedo retrievals and has the potential for application in the field of the regional and global energy budget.
Urban Landscape Change Analysis Using Local Climate Zones and Object-Based Classification in the Salt Lake Metro Region, Utah, USA
Authors: Collins Jed , Dronova Iryna
Published: July 2019

Urban areas globally are vulnerable to warming climate trends exacerbated by their growing populations and heat island effects. The Local Climate Zone (LCZ) typology has become a popular framework for characterizing urban microclimates in different regions using various classification methods, including a widely adopted pixel-based protocol by the World Urban Database and Access Portal Tools (WUDAPT) Project. However, few studies to date have explored the potential of object-based image analysis (OBIA) to facilitate classification of LCZs given their inherent complexity, and few studies have further used the LCZ framework to analyze land cover changes in urban areas over time. This study classified LCZs in the Salt Lake Metro Region, Utah, USA for 1993 and 2017 using a supervised object-based analysis of Landsat satellite imagery and assessed their change during this time frame. The overall accuracy, measured for the most recent classification period (2017), was equal to 64% across 12 LCZs, with most of the error resulting from similarities among highly developed LCZs and non-developed classes with sparse or low-stature vegetation. The observed 1993–2017 changes in LCZs indicated a regional tendency towards primarily suburban, open low-rise development, and large low-rise and paved classes. However, despite the potential for local cooling with landscape transitions likely to increase vegetation cover and irrigation compared to pre-development conditions, summer averages of Landsat-derived top-of-atmosphere brightness temperatures showed a pronounced warming between 1992–1994 and 2016–2018 across the study region, with a 0.1–2.9 °C increase among individual LCZs. Our results indicate that future applications of LCZs towards urban change analyses should develop a stronger understanding of LCZ microclimate sensitivity to changes in size and configuration of urban neighborhoods and regions. Furthermore, while OBIA is promising for capturing the heterogeneous and multi-scale nature of LCZs, its applications could be strengthened by adopting more generalizable approaches for LCZ-relevant segmentation and validation, and by incorporating active remote sensing data to account for the 3D complexity of urban areas.