bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantification analysis of lactate dehydrogenase and C-reactive protein in evaluation of the severity and prognosis of the acute pancreatitis
Authors: Yin Xiang , Xu Jing , Zhang Qi , Yang Li , Duan Yunjie
Published: May 2020

To evaluate the value of C-reactive protein (CRP) and lactate dehydrogenase (LDH) in assessing the severity and prognosis of acute pancreatitis (AP). A retrospective analysis was performed with the clinical data of 115 AP patients who were delivered to this hospital between December 2012 and December 2017 for treatment, in which there were 76 patients with mild AP (MAP group) and 39 with moderately severe AP (MSAP) and severe AP (SAP) (non-MAP group). Within 24 h after admission, we detected the levels of CRP, LDH and amylase (AMY) in serum, and according to the sensitivity and specificity of CRP, LDH and AMY, as well as the SROC curve, we evaluated the diagnostic value of these indicators in assessing the severity of AP. In serum, the levels of CRP and LDH in the non-MAP group were significantly higher than those in the MAP group (P <0.05). And in predicting the development of AP, the sensitivity, specificity and accordance rate of CRP were 59.0%, 97.4% and 84.3%, with a cut-off value of 176.00 mg/L; for LDH, these indexes were 94.9%, 88.2% and 90.4%, with a cut-off value of 235.50 U/L. Thus, LDH, combined with the CRP can well predict the incidence rate of the MODS and mortality rate of AP. Finally, we conclude that within 24 h after admission, the levels of CRP and LDH in patients can serve as indicators for evaluating the severity and prognosis of AP.
Effect of isofebrifugine on the proliferation and invasion of human gastric cancer cells via MMP
Authors: Xu Jin , Wu Jianbo , Tang Chuankang
Published: May 2020

Gastric cancer (GC) is one of the most common fatal cancers among gastrointestinal malignancies. At present, the treatment of gastric cancer involves a combination of surgery and chemotherapy. Isofebrifugine (IFE) is an alkaloid with many biological properties. In this study, results from MTT, scratch and invasion assays showed that IFE significantly inhibited the proliferation, migration and invasion of SGC7901 gastric cancer cells. Through RT-PCR and Western blot experiments, it was revealed that IFE significantly inhibited the mRNA and protein expressions of MMP-2, MMP-9 and SDF-1 which are closely related to cancer invasion and metastasis. Thus, IFE possesses anti-gastric cancer properties.
Antitumor mechanism of MAP30 in bladder cancer T24 cells, and its potential toxic effects in mice
Authors: Shi Zhenduo , Pang Kun , Yang Wen , Zhou Jiahe , Dong Bingzheng , Zhang Zhiguo , Zhao Yan , Han Conghui , Hao Lin
Published: May 2020

To investigate the antitumor mechanism of MAP30 in human bladder cell line (T24) and its potential toxic effects in mice.  In this study, the biological behavior of MAP30's influence on bladder cell was investigated to reveal the antitumor mechanism and role of MAP30 in bladder cancer. MAP30 gene sequence optimized by gene synthesis codon was inserted into the prokaryotic expression vector pET-28a to produce a large amount of target protein in Escherichia coli. The protein product was obtained after purification. Membrane hydration method was used to prepare MAP30 liposome in order to enhance its membrane permeability. The effects of MAP30 on the viability, apoptosis and migration of T24 cell were assessed using 3"‘(4,5"‘dimethyl"‘thiazol"‘2"‘yl)"‘2,5"‘diphenyl"‘2H"‘tetrazolium bromide (MTT), flow cytometric and TUNEL assays, respectively. Mice were transfected with bladder cancer cells for 48 h. The expressions of apoptotic and non-apoptotic proteins were determined using Western blotting. Changes in tumor volume and occurrence of metastasis were assessed using luciferase assay. After 7 days, liver and kidney were excised for histological examination. The levels of reactive oxygen species (ROS), malondialdehyde (MDA), and reduced glutathione (GSH), and activities of catalase and glutathione peroxidase (GPx) were determined in serum or homogenate using enzyme-linked immunosorbent assay (ELISA). The yield of MAP30 after purification was significantly increased. The results of MTT assay showed that MAP30 significantly and concentration-dependently inhibited the proliferation and migration of T24 cells (p < 0.05). The prepared liposomes had uniform hydrated particle size of 132.6 nm, with encapsulation efficiency of 78 %. The inhibitory effect of MAP30 liposome on T24 cells was significantly higher than that of MAP30, and MAP30 significantly increased the number of apoptotic cells (p < 0.05). Western blotting showed that MAP30 significantly promoted the expression of caspase 3 (p < 0.05), but did not significantly affect the expressions of bcl-2 and bax (p > 0.05). It also significantly down-regulated the expressions of NF-kB, JNK and MMP2 (p < 0.05). Tumor formation was significantly inhibited, and tumor volume reduced in bladder cancer-bearing mice after treatment with MAP30 (p < 0.05). Histological examination showed that MAP30 induced mild histological changes in the liver and kidney of mice, and significantly increased the level of MDA at day 1 (p < 0.05). It also significantly and time-dependently increased ROS, but reduced GSH levels and activities of catalase and GPx (p < 0.05). However, MAP30 had no significant effect on DNA (p > 0.05). The apoptotic effect of MAP30 in T24 cells is mediated via activation of caspase-3 signaling pathway. The protein produces mild histological changes in the liver and kidney of mice, but has no significant effect on DNA.
Liposomal clodronate combined with Cisplatin or Sorafenib inhibits hepatocellular carcinoma cell proliferation, migration and invasion by suppressing FOXQ1 expression
Authors: Zhou Yilong , Chen Yuan , Ma Chunyang , Shao Bingfeng , Zhang Feng
Published: May 2020

The purpose of this study was to investigate the effect of liposomal clodronate combined with Cisplatin or Sorafenib on the FOXQ1 expression and biological function of hepatocellular carcinoma cell lines.  The expression of FOXQ1 was determined in normal hepatic cell line and hepatocellular carcinoma cell lines using quantitative real-time polymerase chain reaction (qRT-PCR). HepG2 and MHCC97H cells were administered low, medium and high concentrations of cisplatin (3, 5 and 7 Î¼g/ml) or Sorafenib (2, 7 and 20 Î¼g/ml) in combination with liposomal clodronate (LC, 20Î¼g/ml), and the expression of FOXQ1 in each group was determined. Cell migration, MTT and transwell assays were used to determine the effects of the treatments on biological functions of HepG2 and MHCC97H cells. qRT-PCR showed that the expression of FOXQ1 mRNA was higher in the four hepatocellular carcinoma cell lines than in normal cells, and the expression of FOXQ1 mRNA in HepG2 and MHCC97H cells were more dominant. All the tested doses of Cisplatin, but only high dose Sorafenib down-regulated the expression of FOXQ1. However, Sorafenib at low and medium concentrations had no significant effect on the expression of FOXQ1. When Cisplatin or Sorafenib was administered in combination with LC, the expression level of FOXQ1 was significantly reduced. Cell migration, MTT and transwell assays showed that proliferation, migration and invasion were inhibited when each drug was administered alone, but was stronger when the drugs were combined with liposomal clodronate.Liposomal clodronate combined with Cisplatin or Sorafenib down-regulates the expression of FOXQ1 in HepG2 and MHCC97H hepatoma cells, and inhibits their proliferation, migration and invasion.
Expression study of an Amino Acid Permease-like gene in Phaseolus vulgaris L.
Authors: Patwa Nisha , Chakraborty Brototi , Basak Jolly
Published: May 2020

Amino acid permease-like (AAP-like) gene plays a critical role in absorbing amino acids through roots in plants. A number of studies have been done on amino acids uptake in plants but till date there is no report about the expression of AAP gene in Phaseolus under field allied condition. The aim of this study is to measure the expression of AAP-like gene on alanine, glycine and proline amino acid uptake capacity in Phaseolus vulgaris at field relevant concentrations. Amongst three amino acids, a drastic significant increase of 63.15 fold in expression of AAP-like gene is observed in 50 µM alanine at 2 hr. At 50 µM of proline and 25 µM of alanine, AAP-like gene expression also shows high expression of 43.71 fold at 2 hr and 42.50 fold at 1 hr respectively. This study elucidated the dose dependent relationship of glycine, alanine and proline with the expression of AAP-like gene in amino acid transport in natural conditions in roots of P. vulgaris. Additionally, this research is also useful in identification of plants needing less surplus nitrogen additions and helpful in optimizing fertilizers by tailoring AAP gene expression to match plant uptake capacities in agriculture.
Assessment of exogenous application of plant growth regulators on Cress seed germination and ?-Galactosidase activity
Authors: Alghonmeen Ola O. , Alsharafa Khalid Y , Al-limoun Muhamad O. , Khleifat Khaled M. , Al-Ramamneh Ezz Al-Dein Muhammed
Published: May 2020

Plant growth regulators (PGRs) were involved in several types of abiotic stress responses by means of improving seed germination and modifying the growth and development of medicinally important Lepidium sativum via alleviating the negative effects of abiotic stresses. Therefore, the present research was carried out to investigate the effects of exogenous application of PGRson seed germination, protein content and ?-galactosidase activity of L. sativum. Germination of L. sativum seeds was monitored for a short interval after the start of incubation until growth became 100%. While cytokinin treatment showed a positive effect on seed germination more than Gibberellic acid (GA), salicylic acid (SA) produced a higher negative effect than auxins. Quantifying changes in total protein content during seed germination as influenced by PGRs revealed that all PGRs have to exert a positive effect arranged in the following order: SA ? auxin ? cytokinin ? GA. Parallel to changes in germination percentage and total protein content of seed, a negative effect was attainedon ?-galactosidase specific activity in response to PGRs with the following arrangement: SA ? auxin ? cytokinin ? GA.In conclusion, the present study proposed the potential importance of the type and magnitude of exogenously applied PGRs during the germination of easily or even more difficult-to-germinate seeds.
Our Kids: The American Dream in Crisis – nierówności w koncepcji kapitału społecznego Roberta P. Putnama (perspektywa edukacyjna)
Authors: Kowalski Mirosław , Albański Łukasz
Published: May 2020

This article examines the concept of inequality in Robert D. Putnam’s Our Kids. The article discusses the intersections of social inequality and social capital shown in the book. In particular, the issue of inequality in access to education between children from different backgrounds is outlined and critically discussed. The conclusion suggests a possible new way to look at the relationship between education and inequalities, providing a theoretical basis for educational research.
Ryzyko jako element stylu życia współczesnej młodzieży. Ujęcie teoretyczno-empiryczne
Authors: Paulina Peret-Drążewska
Published: May 2020

The theoretical and empirical analysis is intended to address the specificityof risk categories in the present and the relevance of these issues in the light of their perception by modern youth, which is part of the analysis of young people’s lifestyles. Discussions on changes in the risk category in the present and the distinction between risk and anxiety were also discussed. Also presented are the results of our own research presenting the opinions of contemporary youth on contemporary society, taking into account the category of risk.
Roszczeniowość vs ugodowość. Postawy rodzicielskie w środowiskach szkolnych z perspektywy teorii reprodukcji społeczno-kulturowej Pierre’a Bourdieu
Authors: Marta Jurczak-Morris
Published: May 2020

School is a unique environment of social interactions. It generates the variety of attitudes and behaviours. This paper demonstrates differences in the attitudes towards school of parents, according to their social status. The research study emphasises that social class is one of the key determinants of ways in which parents participate in their children’s education process. Likewise, it shows that educational aspirations of a certain group of parents, as well as their vision of what education should look like vary and make up specific‘class patterns’ of parental participation in education. This qualitative rese-arch clarifiesthat most parents from advantaged social background have a clear vision on education as well as expectations towards school. They are therefore more likely to demand and complain. On the contrary, parents from a lower social class tend to be conciliatory, unquestionably trusting the school institution.
Pojęcie „zdolność” w narracji studentów angielskich i polskich. Ujęcie kognitywne
Authors: Gierczyk Marcin
Published: May 2020

The purpose of this publication is to show how students from Poland and England defineand understand the concept of ‘giftedness’. The analyses result from the author’s research conducted over the years 2013-2016. In the interviews, the students presented their own associations with the matter. Materials collected from 30 respondents, including 15 students from Śląski University and from a Lublin University and 15 students from Oxford University, showed that the concept of ‘giftedness’ is understood by Polish students identically to English students. ‘Giftedness’ was definedby a series of connotations: ‘creativity’, ‘talent’, ‘ability to do something’, ‘predisposition’, and ‘organization of thinking’.
Kształcenie policjantów w Stanach Zjednoczonych (wybrane podejścia i tendencje)
Authors: Andraszczyk Wojciech
Published: May 2020

The article reconstructs selected contexts of police training in the United States of America. The narration concentrates on the historical origins of police training as well as on the dominant relevant trends in presentday American society. Furthermore, the paper seeks to compare the two ways of thinking about police training which influencethe training programs in police academies. The author presents methods of shaping the identity of policemen, the firstof which is a military model and the second one is an academic model. The text focuses moreover on the evolution of the operating system of the American police: from intervention-oriented to a community-oriented model, which is constantly gaining importance in some police training programs.
Współczesne studia doktoranckie i współcześni doktoranci. Doniesienia ze zwiadu badawczego
Authors: Banaszak Sławomir , Andrys Magdalena , Dolata Dorota , Iwanicka Beata , Schmidt Anna , Ulaniecka Natalia
Published: May 2020

The article presents the results of a research study on the situation of contemporary post-graduate students. Focus group interviews helped obtain post-graduate students’ opinions about the conditions of starting and continuing doctoral studies. The discussion focused on three main topics: motivation to start doctoral studies, the quality and conditions of doctoral studies evaluation, and the advantages and disadvantages of undertaking doctoral studies. Analysis of the respondents’ statements shows that the motivations for starting doctoral studies vary strongly. Common in the experience of the interlocutors is a lack of stability in life (including financialstability), insecurity of employment, overload, and stress. However, post-graduate students presented also positive aspects of doctoral studies, e.g.: social prestige or the possibility of development. The last part of the article describes issues related to problems and pathological phenomena (such as ‘punctasis’/‘points, credits collecting’), pointing to systemic and individual limitations affecting the situation of doctoral students in the Polish tertiary education system.
In Defense of Human Identity: the Reality of Globalization as the Cause of Modernization of Modern Educational Systems
Authors: Markiewicz Elżbieta
Published: May 2020

The article In Defense of Human Identity: the Reality of Globalization as the Cause of Modernization of Modern Educational Systems is an attempt to reflect on the role of the educational process in the context of the value system offered in the modern world. It appears that authentic human participation in the reality of global and involuntary compliance with the directives of globalism is both a crisis of identity, of its existence, values, norms, and all human activities. Efforts taken to demonstrate the value of dogma, which carries with it an affirmation of the modern world, and conformist standards of conduct, is to notice the risk of objective truth about man and reality. Civilization implies in fact a kind of order to eradicate what is human. The human drama shows an almost total devotion to the values of functionalism and utility. Therefore, it is vital for modern education to return to the personal dimension of human existence. This accomplishment, a full range of education, which means clarifying the challenges confronting it, is only possible with recognizing and highlighting the personal status of the man based on the personalistic norm. This article helps the contemporary human being to realize the importance of learning to respect human dignity and to protect the identity of a mature man
Reforma szkolna z roku 2016 – troska o ucznia czy innowacja na zamówienie polityczne
Authors: Rondalska Dorota
Published: May 2020

This article is devoted to the school reform of December 2016. The author discusses its appropriateness, the way it is introduced, and the social climate that accompanies it. This reform re-introduces the state from before 1998, i.e. an 8-year-long primary school and eliminates the junior school. The author raises a question about the justification for the reform, assuming that it does not take into account the results of the study on the effects of the 1999 reform, but may be politically-motivated. Another thesis concerns the repetition of the application errors of the previous reform as well as the formal way of introducing changes into the school system, especially the school network. The author points to a great social resistance against the reform, especially the way it is conducted and predicts that due to the hasty implementation of the reform and application errors, its effects may be half-hearted.
Kompetencje komunikacyjne nauczycieli
Authors: Strykowska-Nowakowska Justyna
Published: May 2020

The article discusses the problem of teachers’ communicative competences and presents it from both the theoretical and empirical perspective. The first part of the article helps understand the communication process and defines teachers’ communicative styles at school and barriers for pedagogical communication. The second part presents the results of diagnostic research on selected teachers’ communicative competences. The research problems included: How do teachers understand the communication process at school, and what are the factors determining its level? What communicative styles do teachers use? What are the barriers in communication between teachers and students? The article concludes with remarks and postulates.
Właściwości stygmatu a sytuacja życiowa człowieka zagrożonego stygmatyzacją
Authors: Świgost Magdalena
Published: May 2020

It is stated that the properties of stigma have the key role in perceiving social relations and own life situation by the person who is in danger of the process of social stigma. It is ccognitively interesting how agreeing to the process of social stigma is shown in terms of difficulty that is being activated by specific feature, pressing, in exceptional sense, on seeing and controling the stigma. This article contains the theoretical analysis of the process of social stigma in relation to its chosen features. There has been done an attempt to present mutual assorted interaction with the influence of blocking predicators or those restoring the process of social self-indicating and indicating.
Odpowiedzialność zawodowa, a wprowadzanie uczniów klas IV szkoły podstawowej w świat historii. Oczekiwania społeczne, a codzienna rzeczywistość – wyniki obserwacji młodego nauczyciela
Authors: Hurysz Mateusz
Published: May 2020

The teacher today works in close relationship with others, which forces him to accept a limited set of attitudes and ways of action. I assume for the purposes of this paper two groups of factors for the teacher modeling approach: external – pedagogical supervision and school-imposed regulations deriving from state teachers’ and society’s expectations, especially from parents and students themselves, the internal factors – the process of educating and teaching every teacher and crucial, long-term formation of conscience. Based on this specific quadrangle I will present the first stage of introducing a young man to the world of history at the second stage of compulsory education in Polish schools. The assumption is that a primary school teacher may encourage or discourage the student to continue learning history, both national and global, in the middle school or in the secondary school. This is a great responsibility, which even teachers themselves often forget. The article indicates how all of this corresponds to social expectations and what everyday reality for a young teacher is...
Jedna, wiele czy może żadna? Fenomenologia w naukach o wychowaniu. Wątki fenomenologiczne w naukach o wychowaniu – kontekst historyczny
Authors: Piotrowski Paweł
Published: May 2020

The article presents and reflects on the presence of some phenomenological motifs in educational sciences research and investigations. The text focuses on historical, methodological and structural context of this connection. An attempt is made to assert qualification conditions for each of the answer options mentioned in the title, as possible answers to the question about the connection between phenomenology and educational science. An attempt to define reasons for each of the above answers is taken as well.
’Inquiry Physics’ Program for Students-Implementation of Inquiry Learning in the Physics Lessons in the High School in Israel
Authors: Sigron Michal
Published: May 2020

Teaching of the Sciences recently emphasizes more than in the past the importance of the student’s experience in inquiry, in different places in the world in general and in Israel in particular. This article describes the process of scientific inquiry and the change of the perception of the role, both of the student and of the traditional teacher, in the performance of the inquiry process. The article presents different initiatives that integrate the learning of inquiry in the teaching of physics in the high school in Israel. These initiatives show that the inquiry laboratories have the potential to promote meaningful learning among the students, to increase the depth of their understanding of the scientific concepts and the understanding of the nature of science. The article focuses on ‘Inquiry Physics’ program for students and presents the goals, the target audience, the time framework, and the structure of this program. The program aims to assemble the different initiatives under one framework and to provide them with an appropriate place in the teaching of physics. The learning method of inquiry is now fledgling in the State of Israel and mainly in the knowledge realm of physics. Therefore, exposure to this program may contribute to the body of knowledge on the characteristics of learning and teaching through inquiry in general and on the teaching of physics in particular.
The Learning Community’s Program – a Fresh Approach to the Single Teacher
Authors: Cohen-Brenner Daphne
Published: May 2020

Promoting physics studies depends highly on the teacher’s instruction quality. This paper re-examines the influence of participation in a learning community on the teacher’s professional development. As an outcome, improving his students’ physics learning abilities, in light of theories on the process of change, is obtained thanks to the teacher participating the progra Daphne Cohen Brenner is a physics teacher and district instructor in Israel, leading a professional learning community. The article is based on the Ph.D. thesis written at AMU University in the framework of doctoral studies in education.
The School Experience of Arab LGBT Students in Israel
Authors: Said Anton
Published: May 2020

The purpose of the research is to study the school experience of Arab Lesbians, Gays, Bisexuals and Transgender (LGBT) in the state of Israel from own point of view. The research question is: what is the school experience of Arab LGBT graduates of the Israeli educational system in high schools where the language of learning is Arabic? This is a quantitative research, based on a questionnaire completed by 51 Arab LGBT, graduates of the Israeli educational system. They attended high schools where the language of learning is Arabic; 69.8% (N=37) of them were completed by males, 28.3% (N=15) by females, and 1% (N=1) by transgender. 62.3% reported that their sexual inclination is homosexual, 22.6% lesbian, 13.2% bisexual, and transgender one (1%). The ages of the respondents range from 18 to 32. The research participants were recruited through the ‘snowball sample’ method. The initial request was made to my personal Facebook friends whom I knew were LGBT, and they disseminated the questionnaire to additional LGBT friends they knew. The questionnaire examined the school experience in general and their perception of the role of the Arab educational counselor particularly on the topic of the sexual orientation and sexual identity, attitudes towards LGBT students, and degree of the influence of traditional Arab society on his role as a school counselor whose role is to advise, help, and accompany all the students so as to integrate in the school and in society in the best possible way. 51 questionnaires were examined in three independent variables of respondents on which the research focuses: the sector to which the respondent’s school belongs, the respondent’s religion, and the school location.
Witold Jakubowski (red.) Pedagogika kultury popularnej – teorie, metody i obszary badań, Oficyna Wydawnicza Impuls, Kraków 2016, ss.350
Authors: Włodarczyk Rafał
Published: May 2020

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Sylwester Bębas, Mariusz Z. Jędrzejko, Kaja Kasprzak, Adam Szwedzik, Agnieszka Taper (red.), Cyfrowe dzieci. Zjawisko, uwarunkowania, kluczowe problemy, Wydawnictwo Aspra, Warszawa – Milanówek 2017, ss. 396
Authors: Górka Marek
Published: May 2020

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Sprawozdanie z Ogólnopolskiej Konferencji Naukowej „Nauczyciel. Czas. Przestrzeń. Szkoła (rola, funkcje, zadania dawniej i dziś)”, Zielona Góra, 5 kwietnia 2017 roku
Authors: Kabat Małgorzata , Kobyłecka Ewa
Published: May 2020

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Sprawozdanie z II Ogólnopolskiej Interdyscyplinarnej Konferencji Naukowej „Diagnoza i jej zastosowanie w kontekście jednostkowym i społecznym”, Poznań, 24 maja 2017 roku
Authors: Peret-Drążewska Paulina
Published: May 2020

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Status związku (w związku intymnym vs singiel), konstrukt Ja i zdrowie psychiczne młodzieży akademickiej
Authors: Adamczyk Katarzyna , Jankowiak Barbara , Soroko Emilia
Published: May 2020

The aim of this online survey was to test the hypothesis that self-construals (Independent self-construal and Interdependent self-construal) mediate the association between relationship status (single vs. partnered) and mental health. Four hundred and twenty-nine university students (327 females and 102 males) 19-25 (M = 21.79, SD = 1.72) completed the Polish versions of the General Health Questionnaire-28 and Self-Construal Scale. The results indicated that although different configurtions of self-construals are related to the level of experienced anxiety, the aspects of mental health analyzed are not related to the interaction between relationship status (partnered vs single) and 4 configurationsof self-construals. At the same time, the revealed lack of correlation between relationship status and mental health is contradictory to expectations and hypotheses. The results suggest that university students are perhaps in the middle of a prolonged moratorium, and in consequence, not making an attempt to fulfilldevelopmental tasks characteristic for (young) adults cannot be seen as an indicator of any aspect of psychological health.
Zmysły i edukacja. O roli doświadczenia, wiedzy oraz selekcji (refleksja filozoficzna w edukacji)
Authors: Futyma Sławomir
Published: May 2020

Sensory experience leads to the initiation of a complex process of thinking about the world. The result of this process are the images of what surrounds us. We definethis action as education. Because looking at the world from the perspective of sensual experience is the potential ability of every human being (Hannah Arendt), education becomes a tool enabling the simulation of the existing world and the one that may appear in the future. About who we are and where we are, who we will decide, the quality of the senses. The quality of the senses translates into the value of the cognitive process. The consequence of the quality of the cognitive process is the collection of information and knowledge. This sensual logic inscribes the action that classifiesus people according to predisposition or ava-ilable information that results from the quality of sensual functioning. As Leonardo da Vinci saw it: “Experience, the intermediary between creative nature and the human race, teaches what nature uses among mortals, that before the necessity of necessity one cannot act differently than reason, his teaching works.”
Z historii polskiej surdopedagogiki – trudne początki
Authors: Szczepankowski Bogdan
Published: May 2020

The Institute for the Deaf-mute in Warsaw, established in 1817 by Father Jakub Falkowski, is commonly considered to be the firstschool for deaf-mute children in Poland. However, similar actions had been taken three times earlier: in 1803 in Romanow, Volhynia, in 1804 in Vilnius, and in 1809 in Warsaw. Unfortunately, those activities did not generate the expected effects. The article recalls those unsuccessful attempts of establishing the first schools for deaf children
Jakie prawo jest potrzebne pedagogom?
Authors: Andrzejewski Marek
Published: May 2020

The article discusses the relation between law and pedagogy as well as between lawyers (theoreticians and practitioners) and pedagogues (theoreticians and practitioners). The author describes the manifestations of the current legal crisis and indicates how this process affects social life, particularly its pedagogical aspect. In his opinion, law should ensure freedom of pedagogical initiatives instead – as the dominant trend dictates – restrain them. The article is a changed version of a lecture delivered on the occasion of a jubilee of Professor Wiesław Ambrozik, held on 16 November 2017, in Collegium Minus, Adam Mickiewicz University, Poznań.
FARKLI EKSTRAKSİYON YÖNTEMLERİNİN HİBİSKUS EKSTRAKTLARININ KALİTE ÖZELLİKLERİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI
Authors: DİNÇER Cüneyt , TONGUR Timur , ERKAYMAZ Taner
Published: May 2020

Bu çalışmada farklı ekstraksiyon yöntemlerinin hibiskus ekstraktlarının kalite özellikleri üzerine etkisi araştırılmıştır. Bu amaçla termal ekstraksiyon (40 ve 75 ºC) ve termosonikasyon (0.031 W/mL, 40 ve 75 ºC) uygulamaları gerçekleştirilmiştir. Ekstraksiyon sürecinde 1., 5., 15. 30., 60. ve 120. dk’larda alınan örneklerde, sıcaklık ve süreye bağlı olarak °Brix ve antosiyanin içeriğinde artış belirlenirken, pH önemli bir değişim seyri göstermemiştir. Ardından örnekler 1.1 °Briks’e kadar ekstrakte edilmiştir. Bu örneklerde antosiyanin içeriği 149.7-156.2 mg/L arasında tespit edilirken, delfinidin-3-sambiosit ve siyanidin-3-sambiosit ise sırasıyla 112.1-120.5 ve 52.35-55.26 mg/L arasında belirlenmiştir. Sakaroz, en yüksek miktardaki şeker olarak belirlenirken, malik asit ise 811.2-932.4 mg/L arasında tespit edilmiştir. Ekstraksiyonların kalite özellikleri üzerine etkisi değerlendirildiğinde bulanıklık değerleri hariç, uygulamalar arasında istatistiki açıdan önemli bir fark olmadığı belirlenmiştir.
Isolation and Identification of Actinomycetes Associated with Moss on the Surface of the Borobudur Temple Stone
Authors: Putri Ade Lia , Purbani Debora Christin , Kanti Atit , Kusmiati Mia , Habibi Moh
Published: May 2020

Mosses growing on the surface of the Borobudur Temple will affect the aesthetic value of the temple. Interaction between moss and actinomycetes may trigger the growth of moss that can cause an increase in biodeterioration of stone. The purpose of this study was to isolate and identify the actinomycetes associated with moss on the surface of decayed stone of Borobudur Temple as well to assess their ability for phosphate solubilizing. Actinomycetes were isolated using serial dilution method and were identified based on 16S rRNA gene sequences. A total of 37 actinomycetes were isolated from three sampling sites. The isolates found belong to five genera (Gordonia, Microbacterium, Micromonospora, Nocardia, and Streptomyces) and distributed among four families (Microbacteriaceae, Micromonosporaceae, Nocardiaceae, and Streptomycetaceae). Isolates of actinomycetes composed of 19 Streptomyces Group and 18 Rare Actinomycetes Group. Nineteen isolates (51.35%) were identified as genus Streptomyces. Seventeen isolates (45.94%) showed abilities to release soluble phosphate and most of the isolates belong to the genus Streptomyces. The isolates have been collected will be deposited to Indonesian Culture Collection (InaCC) to enrich the collection of actinomycetes from ancient stone in Indonesia and will be used as a source of reference material research, taxonomic, or as source for further study.
Molecular Barcoding Reveal the Existence of Mole Crabs Emerita emeritus in North Coast of Central Java
Authors: Bhagawati Dian , Winarni Elly Tuti , Nuryanto Agus
Published: May 2020

The existence of mole crabs had been reported from the southern coast of Central Java. However, no mole crab data was available from the northern coast Central Java.  This study aimed to figure out the existence of mole crabs in the northern coast of Central Java as revealed from the cytochrome c oxidase 1 (COI) barcoding. Mole crabs samples collected in Sendang Sikucing Beach Kendal. Taxonomic status of the samples was inferred from sequences similarity test using basic local alignment search tool to conspecific sequence deposited in GenBank. Pairwise genetic distances were calculated based on the Kimura 2-parameter model. A phylogenetic tree had reconstructed in molecular evolution genetic analysis (MEGA) software based on neighbor-joining algorithm. Branching reliability was obtained from 1000 bootstraps replication while branching polarity was obtained from the out-group comparison. The mole crab samples from the northern coast of Central Java had unambiguously identified as Emerita emeritus based on high sequences similarities (98.27%-99.70%), low genetic distances (0.002-0.005), and their monophyly with Emerita emeritus (KR047035) in GenBank. Pairwise analysis among each possibility pair of samples had genetic distances ranged between 0.000 and 0.005, indicated that all sample belong to single species. The data provide the first record of the existence of Emerita emeritus in the northern coast of Central Java. Our data on the existence of E. emeritus in the northern coast of Central Java is among essentials information as a scientific basis in creating policy for the management of sustainable use of the mole crabs in the areas.
Induction and Growth Kinetics Callus of Tomato (Solanum lycopersicum)
Authors: Setiaji Arkan , Annisa RR Rifka , Rumiyati Rumiyati , Semiarti Endang
Published: May 2020

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.
Anthracnose Disease on Vegetables Crops in Serang Village, District of Karangreja, Purbalingga Regency
Authors: Sucianto Eddy Tri , Abbas Muachiroh , Purwati Endang Sri
Published: May 2020

Various vegetables are widely cultivated in Serang Village, District of Karangreja, Purbalingga Regency. This village is located at 1350 above sea level. Vegetable productions in Serang are often constrained by the presence of diseases caused by pathogenic fungi Colletotrichum sp. called anthracnose. However, there was no data regarding anthracnose attack in vegetable crops in Serang. This study aimed to find out the types of vegetable that were attacked by anthracnose disease as well as the intensity of the attack in a vegetable crops in Serang Village, Karangreja District, Purbalingga Regency. Vegetables with anthracnose were purposively selected from 10 different locations. The anthracnose disease was identified based on their symptoms and analyzed descriptively. The results showed that five different vegetable crops suffer from anthracnose, and the intensity of anthracnose ranges from 32% up to 52%. Those intensity percentages indicate that large part of vegetable crops in Serang Village was attacked by anthracnose and possibly cause a significant decrease in vegetable production in that village. Our results provide the first scientific data about anthracnose attack in Serang Village. The data is essential for the management of vegetable crops in Serang Village.
Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers
Authors: Ishak Muhammad Alif , Daryono Budi Setiadi
Published: May 2020

Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds.
The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation
Authors: Mahfudhah Raudhah , Eriani Kartini , Muchlisin Zainal Abidin , Muthmainnah Cut Ruhul
Published: May 2020

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.
Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina
Authors: Kasmiyati Sri , Kristiani Elizabeth Betty Elok , Herawati Maria Marina
Published: May 2020

Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.
Tyrosinase Inhibition, Antiglycation, and Antioxidant Activity of Xylocarpus granatum
Authors: Batubara Irmanida , Mustofa Maily , Wahyuni Wulan Tri , Tilaar Kilala , Nurcholis Waras , Junardy Fransiska Devy , Priyadi Yogo Suro , Subroto Erna , Egra Saat , Zamany Nevianti
Published: May 2020

Xylocarpus granatum is mangrove plant that traditionally used as face powder in Central Sulawesi, Indonesia which related to antioxidant, antiglycation and tyrosinase inhibition activities. This study aimed to evaluate the potency of X. granatum as a tyrosinase inhibitor, antiglycation, and antioxidant. The leaves, stem, stem bark, fruit flesh, fruit peel, and kernel of X. granatum were extracted using ethanol then their tyrosinase inhibition, antiglycation, and antioxidant were evaluated. Tyrosinase inhibition activity was evaluated using in vitro assay with L-tyrosine and L-DOPA as the substrate of monophenolase and diphenolase. Antiglycation activity was studied by measuring the excitation and emission fluorescence from glucose and fructose reaction with Bovine Serum Albumin. Antioxidant activity was measured using the DPPH radical scavenging assay. The result showed that the ethanolic extract of fruit flesh has higher potency as tyrosinase inhibitor (IC50 of 393.8 mg/L and IC50 of 448 mg/L, respectively for monophenolase and diphenolase). Antiglycation assay showed that the ethanolic extract of stem bark provides the strongest antiglycation activity with an IC50 of 118.1 mg/L. Meanwhile, fruit peel provides the strongest antioxidant activity with an IC50 of 5.5 mg/L. Fractionation of ethanolic extracts of each part of X. granatum tree yield fractions with lower bioactivity compared to the crude extract. Moreover, stem extract and fractions from two different locations (Tarakan and Kendari) tend to have different bioactivities strengths.  The stem part of X granatum could be developed as new raw material of cosmetic product in Indonesia, while ethanol as the solvent for extraction, and the different bioactivity of stem extract from different location can be the consideration for the industry to standardize the extract prior to production of final product.
Cytotoxicity and Antiproliferation of Phycocyanin from Spirulina platensis Extract on WiDr Colon Cancer Cell Line
Authors: Putri Ajeng Kurniasari , Dimarti Safira Chairani , Yuniati Renni , Susilaningsih Neni
Published: May 2020

Phycocyanin from Spirulina platensis extract has anticancer activity against various types of cancer cell cultures. However study about its effect on colon cancer cell lines, especially the WiDr, has not been reported before. This study aimed to reveal the anticancer activity of phycocyanin from Spirulina platensis extract on WiDr cells. The research was an in vitro experimental study, with the investigation on cytotoxicity also antiproliferation as the anticancer parameters. Both cytotoxicity and antiproliferation test was conducted through MTT assay to observe the visualization and inhibition of proliferation of different concentrations of phycocyanin in several incubation times on the WiDr colon cancer cell line. The obtained data were then processed statistically with the Two Way ANOVA test at a significance value of p <0.05 and followed with the Post Hoc test since there were significant differences. Based on the results, it could be postulated that phycocyanin extracted from freshwater Spirulina platensis was classified as non-toxic (IC50 of 855 µg/ml). Consequently, it is less potential to be used as the treatment for colon cancer. However, phycocyanin could inhibit the proliferation of the WiDr cell for approximately 47.4%, specifically at the concentration of 1710 µg/ml for 72 hours. It could be concluded that freshwater phycocyanin is less effective as an anticancer substance. The benefit of this study is to provide the new scientific evidence of the contrary results of freshwater phycocyanin activity from Spirulina platensis as an anticancer agent of colon cancer.