bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards an Implantable, Low Flow Micropump That Uses No Power in the Blocked-Flow State
Authors: Johnson Dean , Borkholder David
Published: June 2016

Low flow rate micropumps play an increasingly important role in drug therapy research. Infusions to small biological structures and lab-on-a-chip applications require ultra-low flow rates and will benefit from the ability to expend no power in the blocked-flow state. Here we present a planar micropump based on gallium phase-change actuation that leverages expansion during solidification to occlude the flow channel in the off-power state. The presented four chamber peristaltic micropump was fabricated with a combination of Micro Electro Mechanical System (MEMS) techniques and additive manufacturing direct write technologies. The device is 7 mm × 13 mm × 1 mm (<100 mm3) with the flow channel and exterior coated with biocompatible Parylene-C, critical for implantable applications. Controllable pump rates from 18 to 104 nL/min were demonstrated, with 11.1 ± 0.35 nL pumped per actuation at an efficiency of 11 mJ/nL. The normally-closed state of the gallium actuator prevents flow and diffusion between the pump and the biological system or lab-on-a-chip, without consuming power. This is especially important for implanted applications with periodic drug delivery regimens.
Synthesis and Characterization of New 3-(4-Arylpiperazin-1-yl)-2-hydroxypropyl 4-Propoxybenzoates and Their Hydrochloride Salts
Authors: Marvanova Pavlina , Padrtova Tereza , Pekarek Tomas , Brus Jiri , Czernek Jiri , Mokry Petr , Humpa Otakar , Oravec Michal , Jampilek Josef
Published: June 2016

Five new 3-(4-arylpiperazin-1-yl)-2-hydroxypropyl 4-propoxybenzoates were designed and synthesized as potential dual antihypertensive agents. The compounds were prepared as free bases and subsequently transformed to hydrochloride salts. The position of protonation of nitrogen atoms in the piperazine ring of hydrochloride salts was determined by means of 13C-CP/MAS and 15N-CP/MAS NMR and IR spectroscopy. Using these solid-state analytical techniques, it was found that both nitrogen atoms were protonated when excess hydrogen chloride was used for preparation of salts. On the other hand, when the equimolar amount of hydrogen chloride was used, piperazine nitrogen substituted by aryl was protonated.
A Hydrogel-Based Epirubicin Delivery System for Intravesical Chemotherapy
Authors: Liu Ching-Wen , Wu Yu-Tse , Lin Kai-Jen , Yu Tsan-Jung , Kuo Yu-Liang , Chang Li-Ching
Published: June 2016

This study aimed to examine the efficacy of epirubicin-loaded gelatin hydrogel (EPI-H) in the treatment of superficial urothelium carcinoma. Hydrogel was prepared by Schiff base-crosslinking of gelatin with glutaraldehyde. EPI-H exhibited high entrapment efficiency (59.87% ± 0.51%). EPI-H also increased epirubicin accumulation in AY-27 cells when compared with the effect of aqueous solutions of epirubicin (EPI-AQ); respective epirubicin-positive cell counts were 69.0% ± 7.6% and 38.3% ± 5.8%. EPI-H also exhibited greater cytotoxicity against AY-27 cells than that of EPI-AQ; IC50 values were 13.1 ± 1.1 and 7.5 ± 0.3 μg/mL, respectively. Cystometrograms showed that EPI-H reduced peak micturition, threshold pressures, and micturition duration, and that it increased bladder compliance more so than EPI-AQ. EPI-H enhanced epirubicin penetration into basal cells of urothelium in vivo, whereas EPI-AQ did so only to the umbrella cells. EPI-H inhibited tumor growth upon intravesical instillation to tumor-bearing bladder of F344 rats, inducing higher levels of caspase-3 expression than that observed with EPI-AQ treatment; the number of caspase-3 positive cells in treated urothelium carcinoma was 13.9% ± 4.0% (EPI-AQ) and 34.1% ± 1.0%, (EPI-H). EPI-H has value as an improved means to administer epirubicin in intravesical instillation treatments for bladder cancer.
Sorbicillinoids from Fungi and Their Bioactivities
Authors: Meng Jiajia , Wang Xiaohan , Xu Dan , Fu Xiaoxiang , Zhang Xuping , Lai Daowan , Zhou Ligang , Zhang Guozhen
Published: June 2016

Sorbicillinoids are important hexaketide metabolites derived from fungi. They have a variety of biological activities including cytotoxic, antioxidant, antiviral and antimicrobial activity. The unique structural features of the sorbicillinoids make them attractive candidates for developing new pharmaceutical and agrochemical agents. About 90 sorbicillinoids have been reported in the past few decades. This mini-review aims to briefly summarize their occurrence, structures, and biological activities.
Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes
Authors: Liu Hongzhi , Jiang Nan , Liu Li , Sheng Xiaojing , Shi Aimin , Hu Hui , Yang Ying , Wang Qiang
Published: June 2016

The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC) were investigated in this study. A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X1), extraction time (X2) and ratio of water to raw material (X3). The optimum conditions were 85 °C, 3 h and 20:1 (mL/g) respectively. Regression analysis was done to reveal the experimental results which include 34.97% extraction rate while the value verified under these conditions was 34.49%. The crude PPC was sequentially further purified by Sephadex G-100 chromatography, and one purified fraction was obtained. The PPC purified fraction was characterized by FT-IR, HPAEC; SEC-MALLS. The average molecular weight of the PPC purified fraction was 2.383 × 105 Da. The polysaccharide was mainly composed of glucose, galactose, arabinose and xylose. The PPC have the typical absorption of polysaccharide.
Simultaneous Determination of Purpurin, Munjistin and Mollugin in Rat Plasma by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry: Application to a Pharmacokinetic Study after Oral Administration of Rubia cordifolia L. Extract
Authors: Gao Mingjie , Yang Jing , Wang Zhibin , Yang Bingyou , Kuang Haixue , Liu Lu , Wang Liqian , Yang Chunjuan
Published: June 2016

A specific, simple, sensitive Ultra High Performance Liquid Chromatography-tandem Mass Spectrometry (UHPLC-MS/MS) method has been developed and validated for the simultaneous determination and pharmacokinetic study of purpurin, munjistin, and mollugin in rat plasma. Chromatographic separation was carried out using a C18 column (ACQUITY UPLC® HSS T3, 1.8 μm, 2.1 × 100 mm) with gradient elution. The compounds were detected on a 6430 triple-quadrupole tandem MS with an electrospray ionization (ESI) interface using multiple reaction monitoring (MRM) in positive ionization mode. The samples were prepared by a liquid-liquid extraction (LLE) method with ethyl acetate after being spiked with an internal standard (bifendate). The current UHPLC-MS/MS assay was validated for its linearity, intra-day and inter-day precisions, accuracy, extraction recovery, matrix effect and stability in different conditions. The method was linear for all analytes over the investigated range with all determined correlation coefficients exceeding 0.9900. The intra-day and inter-day precisions were in the range of 4.21% to 14.84%, and the relative errors of accuracies were in the range of −14.05% to 14.75%. The mean recoveries and matrix effects of purpurin, munjistin, and mollugin were higher than 78.87% and 92.56%, repectively. After oral administration of 0.82 g/kg of Rubia cordifolia extract, the maximum plasma concentrations (Cmax) were 70.10 ± 11.78 ng/mL for purpurin, 26.09 ± 6.6 ng/mL for munjistin, and 52.10 ± 6.71 ng/mL for mollugin. The time for maximal concentration (Tmax) was 1.61 ± 0.24 h for purpurin, 2.58 ± 0.19 h for munjistin, and 1.99 ± 0.21 h for mollugin. The established method was further applied to a pharmacokinetic study of purpurin, munjistin, and mollugin in rat plasma. It was concluded from the pharmacokinetic parameters that the three analytes showed a process of slow absorption and metabolism after oral administration of R. cordifolia extract to rats.
Fluorescent Nanocomposite of Embedded Ceria Nanoparticles in Crosslinked PVA Electrospun Nanofibers
Authors: Shehata Nader , Gaballah Soha , Samir Effat , Hamed Aya , Saad Marwa
Published: June 2016

This paper introduces a new fluorescent nanocomposite of electrospun biodegradable nanofibers embedded with optical nanoparticles. In detail, this work introduces the fluorescence properties of PVA nanofibers generated by the electrospinning technique with embedded cerium oxide (ceria) nanoparticles. Under near-ultra violet excitation, the synthesized nanocomposite generates a visible fluorescent emission at 520 nm, varying its intensity peak according to the concentration of in situ embedded ceria nanoparticles. This is due to the fact that the embedded ceria nanoparticles have optical tri-valiant cerium ions, associated with formed oxygen vacancies, with a direct allowed bandgap around 3.5 eV. In addition, the impact of chemical crosslinking of the PVA on the fluorescence emission is studied in both cases of adding ceria nanoparticles in situ or of a post-synthesis addition via a spin-coating mechanism. Other optical and structural characteristics such as absorbance dispersion, direct bandgap, FTIR spectroscopy, and SEM analysis are presented. The synthesized optical nanocomposite could be helpful in different applications such as environmental monitoring and bioimaging.
Crucial Role of Vitamin D in the Musculoskeletal System
Authors: Wintermeyer Elke , Ihle Christoph , Ehnert Sabrina , Stöckle Ulrich , Ochs Gunnar , De Zwart Peter , Flesch Ingo , Bahrs Christian , Nussler Andreas
Published: June 2016

Vitamin D is well known to exert multiple functions in bone biology, autoimmune diseases, cell growth, inflammation or neuromuscular and other immune functions. It is a fat-soluble vitamin present in many foods. It can be endogenously produced by ultraviolet rays from sunlight when the skin is exposed to initiate vitamin D synthesis. However, since vitamin D is biologically inert when obtained from sun exposure or diet, it must first be activated in human beings before functioning. The kidney and the liver play here a crucial role by hydroxylation of vitamin D to 25-hydroxyvitamin D in the liver and to 1,25-dihydroxyvitamin D in the kidney. In the past decades, it has been proven that vitamin D deficiency is involved in many diseases. Due to vitamin D’s central role in the musculoskeletal system and consequently the strong negative impact on bone health in cases of vitamin D deficiency, our aim was to underline its importance in bone physiology by summarizing recent findings on the correlation of vitamin D status and rickets, osteomalacia, osteopenia, primary and secondary osteoporosis as well as sarcopenia and musculoskeletal pain. While these diseases all positively correlate with a vitamin D deficiency, there is a great controversy regarding the appropriate vitamin D supplementation as both positive and negative effects on bone mineral density, musculoskeletal pain and incidence of falls are reported.
Age-Dependent Changes in Resting Energy Expenditure (REE): Insights from Detailed Body Composition Analysis in Normal and Overweight Healthy Caucasians
Authors: Geisler Corinna , Braun Wiebke , Pourhassan Maryam , Schweitzer Lisa , Glüer Claus-Christian , Bosy-Westphal Anja , Müller Manfred
Published: June 2016

Age-related changes in organ and tissue masses may add to changes in the relationship between resting energy expenditure (REE) and fat free mass (FFM) in normal and overweight healthy Caucasians. Secondary analysis using cross-sectional data of 714 healthy normal and overweight Caucasian subjects (age 18–83 years) with comprehensive information on FFM, organ and tissue masses (as assessed by magnetic resonance imaging (MRI)), body density (as assessed by Air Displacement Plethysmography (ADP)) and hydration (as assessed by deuterium dilution (D2O)) and REE (as assessed by indirect calorimetry). High metabolic rate organs (HMR) summarized brain, heart, liver and kidney masses. Ratios of HMR organs and muscle mass (MM) in relation to FFM were considered. REE was calculated (REEc) using organ and tissue masses times their specific metabolic rates. REE, FFM, specific metabolic rates, the REE-FFM relationship, HOMA, CRP, and thyroid hormone levels change with age. The age-related decrease in FFM explained 59.7% of decreases in REE. Mean residuals of the REE-FFM association were positive in young adults but became negative in older subjects. When compared to young adults, proportions of MM to FFM decreased with age, whereas contributions of liver and heart did not differ between age groups. HOMA, TSH and inflammation (plasma CRP-levels) explained 4.2%, 2.0% and 1.4% of the variance in the REE-FFM residuals, but age and plasma T3-levels had no effects. HMR to FFM and MM to FFM ratios together added 11.8% on to the variance of REE-FFM residuals. Differences between REE and REEc increased with age, suggesting age-related changes in specific metabolic rates of organs and tissues. This bias was partly explained by plasmaT3-levels. Age-related changes in REE are explained by (i) decreases in fat free mass; (ii) a decrease in the contributions of organ and muscle masses to FFM; and (iii) decreases in specific organ and tissue metabolic rates. Age-dependent changes in the REE-FFMassociation are explained by composition of FFM, inflammation and thyroid hormones.
The Relationship between Vitamin D Status and Allergic Diseases in New Zealand Preschool Children
Authors: Cairncross Carolyn , Grant Cameron , Stonehouse Welma , Conlon Cath , McDonald Barry , Houghton Lisa , Eyles Darryl , Camargo Carlos , Coad Jane , Von Hurst Pamela
Published: June 2016

Recent research on vitamin D in young children has expanded from bone development to exploring immunomodulatory effects. Our aim was to investigate the relationship of vitamin D status and allergic diseases in preschool-aged children in New Zealand. Dried capillary blood spots were collected from 1329 children during late-winter to early-spring for 25(OH)D measurement by LC-MS/MS. Caregivers completed a questionnaire about their child’s recent medical history. Analysis was by multivariable logistic regression. Mean 25(OH)D concentration was 52(SD19) nmol/L, with 7% of children <25 nmol/L and 49% <50 nmol/L. Children with 25(OH)D concentrations ≥75 nmol/L (n = 29) had a two-fold increased risk for parent-report of doctor-diagnosed food allergy compared to children with 25(OH)D 50–74.9 nmol/L (OR = 2.21, 1.33–3.68, p = 0.002). No associations were present between 25(OH)D concentration and presence of parent-reported eczema, allergic rhinoconjunctivitis or atopic asthma. Vitamin D deficiency was not associated with several allergic diseases in these New Zealand preschool children. In contrast, high 25(OH)D concentrations were associated with a two-fold increased risk of parental-report food allergy. This increase supports further research into the association between vitamin D status and allergic disease in preschool children.
Protein Supplementation Does Not Further Increase Latissimus Dorsi Muscle Fiber Hypertrophy after Eight Weeks of Resistance Training in Novice Subjects, but Partially Counteracts the Fast-to-Slow Muscle Fiber Transition
Authors: Paoli Antonio , Pacelli Quirico , Cancellara Pasqua , Toniolo Luana , Moro Tatiana , Canato Marta , Miotti Danilo , Neri Marco , Morra Aldo , Quadrelli Marco , Reggiani Carlo
Published: June 2016

The response to resistance training and protein supplementation in the latissimus dorsi muscle (LDM) has never been investigated. We investigated the effects of resistance training (RT) and protein supplementation on muscle mass, strength, and fiber characteristics of the LDM. Eighteen healthy young subjects were randomly assigned to a progressive eight-week RT program with a normal protein diet (NP) or high protein diet (HP) (NP 0.85 vs. HP 1.8 g of protein·kg−1·day−1). One repetition maximum tests, magnetic resonance imaging for cross-sectional muscle area (CSA), body composition, and single muscle fibers mechanical and phenotype characteristics were measured. RT induced a significant gain in strength (+17%, p < 0.0001), whole muscle CSA (p = 0.024), and single muscle fibers CSA (p < 0.05) of LDM in all subjects. Fiber isometric force increased in proportion to CSA (+22%, p < 0.005) and thus no change in specific tension occurred. A significant transition from 2X to 2A myosin expression was induced by training. The protein supplementation showed no significant effects on all measured outcomes except for a smaller reduction of 2X myosin expression. Our results suggest that in LDM protein supplementation does not further enhance RT-induced muscle fiber hypertrophy nor influence mechanic muscle fiber characteristics but partially counteracts the fast-to-slow fiber shift.
Protein-Pacing and Multi-Component Exercise Training Improves Physical Performance Outcomes in Exercise-Trained Women: The PRISE 3 Study
Authors: Arciero Paul , Ives Stephen , Norton Chelsea , Escudero Daniela , Minicucci Olivia , O’Brien Gabe , Paul Maia , Ormsbee Michael , Miller Vincent , Sheridan Caitlin , He Feng
Published: June 2016

The beneficial cardiometabolic and body composition effects of combined protein-pacing (P; 5–6 meals/day at 2.0 g/kg BW/day) and multi-mode exercise (resistance, interval, stretching, endurance; RISE) training (PRISE) in obese adults has previously been established. The current study examines PRISE on physical performance (endurance, strength and power) outcomes in healthy, physically active women. Thirty exercise-trained women (>4 days exercise/week) were randomized to either PRISE (n = 15) or a control (CON, 5–6 meals/day at 1.0 g/kg BW/day; n = 15) for 12 weeks. Muscular strength (1-RM bench press, 1-RM BP) endurance (sit-ups, SUs; push-ups, PUs), power (bench throws, BTs), blood pressure (BP), augmentation index, (AIx), and abdominal fat mass were assessed at Weeks 0 (pre) and 13 (post). At baseline, no differences existed between groups. Following the 12-week intervention, PRISE had greater gains (p < 0.05) in SUs, PUs (6 ± 7 vs. 10 ± 7, 40%; 8 ± 13 vs. 14 ± 12, 43% ∆reps, respectively), BTs (11 ± 35 vs. 44 ± 34, 75% ∆watts), AIx (1 ± 9 vs. −5 ± 11, 120%), and DBP (−5 ± 9 vs. −11 ± 11, 55% ∆mmHg). These findings suggest that combined protein-pacing (P; 5–6 meals/day at 2.0 g/kg BW/day) diet and multi-component exercise (RISE) training (PRISE) enhances muscular endurance, strength, power, and cardiovascular health in exercise-trained, active women.
Nutrition in Pediatric Inflammatory Bowel Disease: From Etiology to Treatment. A Systematic Review
Authors: Penagini Francesca , Dilillo Dario , Borsani Barbara , Cococcioni Lucia , Galli Erica , Bedogni Giorgio , Zuin Giovanna , Zuccotti Gian
Published: June 2016

Nutrition is involved in several aspects of pediatric inflammatory bowel disease (IBD), ranging from disease etiology to induction and maintenance of disease. With regards to etiology, there are pediatric data, mainly from case-control studies, which suggest that some dietary habits (for example consumption of animal protein, fatty foods, high sugar intake) may predispose patients to IBD onset. As for disease treatment, exclusive enteral nutrition (EEN) is an extensively studied, well established, and valid approach to the remission of pediatric Crohn’s disease (CD). Apart from EEN, several new nutritional approaches are emerging and have proved to be successful (specific carbohydrate diet and CD exclusion diet) but the available evidence is not strong enough to recommend this kind of intervention in clinical practice and new large experimental controlled studies are needed, especially in the pediatric population. Moreover, efforts are being made to identify foods with anti-inflammatory properties such as curcumin and long-chain polyunsaturated fatty acids n-3, which can possibly be effective in maintenance of disease. The present systematic review aims at reviewing the scientific literature on all aspects of nutrition in pediatric IBD, including the most recent advances on nutritional therapy.
Data-Driven Design of Intelligent Wireless Networks: An Overview and Tutorial
Authors: Kulin Merima , Fortuna Carolina , De Poorter Eli , Deschrijver Dirk , Moerman Ingrid
Published: June 2016

Data science or “data-driven research” is a research approach that uses real-life data to gain insight about the behavior of systems. It enables the analysis of small, simple as well as large and more complex systems in order to assess whether they function according to the intended design and as seen in simulation. Data science approaches have been successfully applied to analyze networked interactions in several research areas such as large-scale social networks, advanced business and healthcare processes. Wireless networks can exhibit unpredictable interactions between algorithms from multiple protocol layers, interactions between multiple devices, and hardware specific influences. These interactions can lead to a difference between real-world functioning and design time functioning. Data science methods can help to detect the actual behavior and possibly help to correct it. Data science is increasingly used in wireless research. To support data-driven research in wireless networks, this paper illustrates the step-by-step methodology that has to be applied to extract knowledge from raw data traces. To this end, the paper (i) clarifies when, why and how to use data science in wireless network research; (ii) provides a generic framework for applying data science in wireless networks; (iii) gives an overview of existing research papers that utilized data science approaches in wireless networks; (iv) illustrates the overall knowledge discovery process through an extensive example in which device types are identified based on their traffic patterns; (v) provides the reader the necessary datasets and scripts to go through the tutorial steps themselves.
How Accurately Can Your Wrist Device Recognize Daily Activities and Detect Falls?
Authors: Gjoreski Martin , Gjoreski Hristijan , Luštrek Mitja , Gams Matjaž
Published: June 2016

Although wearable accelerometers can successfully recognize activities and detect falls, their adoption in real life is low because users do not want to wear additional devices. A possible solution is an accelerometer inside a wrist device/smartwatch. However, wrist placement might perform poorly in terms of accuracy due to frequent random movements of the hand. In this paper we perform a thorough, large-scale evaluation of methods for activity recognition and fall detection on four datasets. On the first two we showed that the left wrist performs better compared to the dominant right one, and also better compared to the elbow and the chest, but worse compared to the ankle, knee and belt. On the third (Opportunity) dataset, our method outperformed the related work, indicating that our feature-preprocessing creates better input data. And finally, on a real-life unlabeled dataset the recognized activities captured the subject’s daily rhythm and activities. Our fall-detection method detected all of the fast falls and minimized the false positives, achieving 85% accuracy on the first dataset. Because the other datasets did not contain fall events, only false positives were evaluated, resulting in 9 for the second, 1 for the third and 15 for the real-life dataset (57 days data).
Guided-Mode-Leaky-Mode-Guided-Mode Fiber Interferometer and Its High Sensitivity Refractive Index Sensing Technology
Authors: Wang Qi , Li Chunyue , Zhao Chengwu , Li Weizheng
Published: June 2016

A cascaded symmetrical dual-taper Mach-Zehnder interferometer structure based on guided-mode and leaky-mode interference is proposed in this paper. Firstly, the interference spectrum characteristics of interferometer has been analyzed by the Finite Difference-Beam Propagation Method (FD-BPM). When the diameter of taper waist is 20 μm–30 μm, dual-taper length is 1 mm and taper distance is 4 cm–6 cm, the spectral contrast is higher, which is suitable for sensing. Secondly, experimental research on refractive index sensitivity is carried out. A refractive index sensitivity of 62.78 nm/RIU (refractive index unit) can achieved in the RI range of 1.3333–1.3792 (0%~25% NaCl solution), when the sensor structure parameters meet the following conditions: diameter of taper waist is 24 μm, dual-taper length is 837 μm and taper distance is 5.5 cm. The spectrum contrast is 0.8 and measurement resolution is 1.6 × 10−5 RIU. The simulation analysis is highly consistent with experimental results. Research shows that the sensor has promising application in low RI fields where high-precision measurement is required due to its high sensitivity and stability.
TripSense: A Trust-Based Vehicular Platoon Crowdsensing Scheme with Privacy Preservation in VANETs
Authors: Hu Hao , Lu Rongxing , Huang Cheng , Zhang Zonghua
Published: June 2016

In this paper, we propose a trust-based vehicular platoon crowdsensing scheme, named TripSense, in VANET. The proposed TripSense scheme introduces a trust-based system to evaluate vehicles’ sensing abilities and then selects the more capable vehicles in order to improve sensing results accuracy. In addition, the sensing tasks are accomplished by platoon member vehicles and preprocessed by platoon head vehicles before the data are uploaded to server. Hence, it is less time-consuming and more efficient compared with the way where the data are submitted by individual platoon member vehicles. Hence it is more suitable in ephemeral networks like VANET. Moreover, our proposed TripSense scheme integrates unlinkable pseudo-ID techniques to achieve PM vehicle identity privacy, and employs a privacy-preserving sensing vehicle selection scheme without involving the PM vehicle’s trust score to keep its location privacy. Detailed security analysis shows that our proposed TripSense scheme not only achieves desirable privacy requirements but also resists against attacks launched by adversaries. In addition, extensive simulations are conducted to show the correctness and effectiveness of our proposed scheme.
An Exact Formula for Calculating Inverse Radial Lens Distortions
Authors: Drap Pierre , Lefèvre Julien
Published: June 2016

This article presents a new approach to calculating the inverse of radial distortions. The method presented here provides a model of reverse radial distortion, currently modeled by a polynomial expression, that proposes another polynomial expression where the new coefficients are a function of the original ones. After describing the state of the art, the proposed method is developed. It is based on a formal calculus involving a power series used to deduce a recursive formula for the new coefficients. We present several implementations of this method and describe the experiments conducted to assess the validity of the new approach. Such an approach, non-iterative, using another polynomial expression, able to be deduced from the first one, can actually be interesting in terms of performance, reuse of existing software, or bridging between different existing software tools that do not consider distortion from the same point of view.
Molecular Characterization of Three Novel Phospholipase A2 Proteins from the Venom of Atheris chlorechis, Atheris nitschei and Atheris squamigera
Authors: Wang He , Chen Xiaole , Zhou Mei , Wang Lei , Chen Tianbao , Shaw Chris
Published: June 2016

Secretory phospholipase A2 (sPLA2) is known as a major component of snake venoms and displays higher-order catalytic hydrolysis functions as well as a wide range of pathological effects. Atheris is not a notoriously dangerous genus of snakes although there are some reports of fatal cases after envenomation due to the effects of coagulation disturbances and hemorrhaging. Molecular characterization of Atheris venom enzymes is incomplete and there are only a few reports in the literature. Here, we report, for the first time, the cloning and characterization of three novel cDNAs encoding phospholipase A2 precursors (one each) from the venoms of the Western bush viper (Atheris chlorechis), the Great Lakes bush viper (Atheris nitschei) and the Variable bush viper (Atheris squamigera), using a “shotgun cloning” strategy. Open-reading frames of respective cloned cDNAs contained putative 16 residue signal peptides and mature proteins composed of 121 to 123 amino acid residues. Alignment of mature protein sequences revealed high degrees of structural conservation and identity with Group II venom PLA2 proteins from other taxa within the Viperidae. Reverse-phase High Performance Liquid Chromatography (HPLC) profiles of these three snake venoms were obtained separately and chromatographic fractions were assessed for phospholipase activity using an egg yolk suspension assay. The molecular masses of mature proteins were all identified as approximately 14 kDa. Mass spectrometric analyses of the fractionated oligopeptides arising from tryptic digestion of intact venom proteins, was performed for further structural characterization.
Mitochondrion: A Promising Target for Nanoparticle-Based Vaccine Delivery Systems
Authors: Wen Ru , Umeano Afoma , Francis Lily , Sharma Nivita , Tundup Smanla , Dhar Shanta
Published: June 2016

Vaccination is one of the most popular technologies in disease prevention and eradication. It is promising to improve immunization efficiency by using vectors and/or adjuvant delivery systems. Nanoparticle (NP)-based delivery systems have attracted increasing interest due to enhancement of antigen uptake via prevention of vaccine degradation in the biological environment and the intrinsic immune-stimulatory properties of the materials. Mitochondria play paramount roles in cell life and death and are promising targets for vaccine delivery systems to effectively induce immune responses. In this review, we focus on NPs-based delivery systems with surfaces that can be manipulated by using mitochondria targeting moieties for intervention in health and disease.
Establishment and Characterization of New Canine and Feline Osteosarcoma Primary Cell Lines
Authors: Meyer Florian , Walter Ingrid
Published: June 2016

Osteosarcomas are the most abundant form of bone malignancies in multiple species. Canine osteosarcomas are considered a valuable model for human osteosarcomas because of their similar features. Feline osteosarcomas, on the other hand, are rarely studied but have interesting characteristics, such as a better survival prognosis than dogs or humans, and less likelihood of metastasis. To enable experimental approaches to study these differences we have established five new canine osteosarcoma cell lines out of three tumors, COS_1186h, COS_1186w, COS_1189, and COS_1220, one osteosarcoma-derived lung metastasis, COS_1033, and two new feline osteosarcoma cell lines, FOS_1077 and FOS_1140. Their osteogenic and neoplastic origin, as well as their potential to produce calcified structures, was determined by the markers osteocalcin, osteonectin, tissue unspecific alkaline phosphatase, p53, cytokeratin, vimentin, and alizarin red. The newly developed cell lines retained most of their markers in vitro but only spontaneously formed spheroids produced by COS_1189 showed calcification in vitro.
Southern Africa’s Water–Energy Nexus: Towards Regional Integration and Development
Authors: Mabhaudhi Tafadzwanashe , Mpandeli Sylvester , Madhlopa Amos , Modi Albert , Backeberg Gerhard , Nhamo Luxon
Published: June 2016

The Southern African Development Community’s (SADC) water and energy sectors are under increasing pressure due to population growth and agricultural and industrial development. Climate change is also negatively impacting on the region’s water and energy resources. As the majority of SADC’s population lives in poverty, regional development and integration are underpinned by water and energy security as the watercourses in the region are transboundary in nature. This paper reviews the region’s water and energy resources and recommends policies based on the water–energy nexus approach. This is achieved by reviewing literature on water and energy resources as well as policy issues. Water resources governance provides a strong case to create a water–energy nexus platform to support regional planning and integration as SADC countries share similar climatic and hydrological conditions. However, there has been a gap between water and energy sector planning in terms of policy alignment and technical convergence. These challenges hinder national policies on delivering economic and social development goals, as well as constraining the regional goal of greater integration. Regional objectives on sustainable energy and access to clean water for all can only be achieved through the recognition of the water–energy nexus, championed in an integrated and sustainable manner. A coordinated regional water–energy nexus approach stimulates economic growth, alleviates poverty and reduces high unemployment rates. The shared nature of water and energy resources requires far more transboundary water–energy nexus studies to be done in the context of regional integration and policy formulation.
Evaluation of Water Efficiency in Green Building in Taiwan
Authors: Cheng Cheng-Li , Peng Jr-Jie , Ho Ming-Chin , Liao Wan-Ju , Chern Szu-Jen
Published: June 2016

Low carbon policies, including those aimed at increasing water efficiency, have been adopted as a crucial strategy for combating global warming and climate change. The green building evaluation system used in Taiwan was first applied in 1999 and initially utilized a building’s water efficiency as the threshold index for determining the building’s environmental impact. Since 1999, more than a thousand buildings have been certified as green buildings using this evaluation system. The quantitative effects of water conservation efforts should be provided to policy makers as a form of positive feedback. To that end, the present study offers a calculation process for estimating the quantitative volume of water saved by practical green buildings. The baseline water usage for all kinds of buildings was determined to serve as the criterion for determining the water-saving efficiency of individual buildings. An investigation of the average water-saving rate from 2000 to 2013 for 1320 buildings certified as green buildings was also conducted to validate the estimation results and found that these green buildings saved an average of approximately 37.6% compared to the baseline water usage rate for all buildings. Water savings will inevitably follow from the use of water-saving appliances or water-saving designs for buildings. The proposed calculation process can be used to clarify the relationships between specific water-saving concepts and the real water usage efficiency of green buildings.
Effect of single dose radiation therapy on weight-bearing lameness in dogs with elbow osteoarthritis
Authors: Nordquist Barbro , Garcia Tanya , Griffin Maureen , Theon Alain , Kim Sun , Hayashi Kei , Kapatkin Amy
Published: June 2016

Objectives: To determine if a single low dose of radiation therapy in dogs with osteoarthritis of the elbow joint was associated with a detectable improvement in their lameness and pain as documented by force platform gait analysis.

Methods: In this cohort longitudinal observational study, five Labrador Retrievers with lameness due to elbow osteoarthritis that was unresponsive to medical treatment were removed from all non-steroidal anti-inflammatory and analgesic medications. A single treatment of radiation therapy delivering 10 Gray was performed on the affected elbow joint(s). Force platform gait analysis was used to assess the ground reaction forces of a limb affected with elbow osteoarthritis both before and after radiation therapy.

Results: Significant differences occurred in the weight-bearing on an affected limb with elbow osteoarthritis after radiation therapy at weeks six and 14. Change due to treatment was particularly apparent in dogs with unilateral elbow osteoarthritis.

Clinical significance: Administering a single low dose of radiation therapy may have a short-term benefit in dogs with elbow osteoarthritis, which is similar to the evidence supporting the use of radiation therapy in horses with orthopaedic disease.
Hemiepiphysiodesis for the correction of proximal tibial valgus in growing dogs
Authors: Olsen Anastasia , Vezzoni Luca , Ferretti Antonio , Palmer Ross , Vezzoni Aldo , Duerr Felix
Published: June 2016

Objectives: To describe the use of hemiepiphysiodesis for the treatment of proximal tibial deformities in immature dogs and evaluate the effect on the mechanical medial proximal tibial angle (mMPTA).

Methods: Skeletally immature dogs with proximal tibial deformities from three institutions treated with hemiepiphysiodesis between March 2006 and January 2015 were included. All dogs were required to have an mMPTA outside the previously published reference range (93.3 ± 1.78°) preoperatively. Dogs were required to have radiographs or computed tomography performed preoperatively and at least eight weeks postoperatively.

Results: A total of 19 dogs (n = 31 limbs) fulfilled the inclusion criteria. The mean mMPTA was 102.5° ± 5.3° preoperatively and 92.4° ± 7.2° at the final re-evaluation. The mean difference in mMPTA was -10 ± 5.1° (range, -1 to -19°; p <0.001). Overcorrection was observed in 16 limbs and mMPTA remained above the reference range in nine limbs. Rebound growth was observed in eight limbs where implant removal was performed.

Clinical significance: Hemiepiphysiodesis for the treatment of proximal tibial valgus is a technique that allows for reduction in mMPTA and should be considered as an early treatment for immature animals that are presented with proximal tibial deformities. Serial radiographs to monitor for overcorrection should be performed. Implant removal should be considered if overcorrection occurs, taking into consideration that rebound growth may be observed.

A Supplementary Table for this paper is available online at: http://dx.doi.org/10.3415/VCOT-15-12-0204
Establishment of normal anatomical radial angles in cats
Authors: Durand Alexane , Parkin Tim , Broome Cameron , De Lima Dantas Brigite
Published: June 2016

Objectives: 1) To describe a radiographic method for determination of joint orientation lines and anatomical joint angles in orthogonal planes of feline radii; 2) to establish a range of normal radial joint orientation angles and anatomical axes in a feline population; and 3) to assess the repeatability and reliability of this methodology.

Methods: The radial anatomical axis, elbow and carpal joint reference lines, and the intersecting angles of each: anatomical medial proximal (aMPRA) and lateral distal radial angles (aLDRA), anatomical caudal proximal (aCdPRA) and distal radial angles (aCdDRA), and sagittal procurvatum (SP) were determined on the orthogonal radiographs of 14 feline limbs. Intra- and inter-observer agreement was determined based on repeated independent readings by two observers using Bland-Altman plots.

Results: The mean ± standard deviation (SD) and 95% confidence interval (CI) for the feline radii were: aMPRA 70.97 ± 3.38° (70.07 – 71.88°), aLDRA 91.72 ± 3.26° (90.84 – 92.59°), aCdPRA 100.5 ± 3.14° (99.62 – 101.3º), aCdDRA 79.95 ± 3.77° (78.94 – 80.96°) and SP 11.07 ± 1.87° (10.57 – 11.58°). The highest mean bias found for both observers was -1.6 to -1.8° for the angle aCdDRA. Sagittal procurvatum had the lowest mean bias for intra- and inter- observer.

Clinical significance: The results obtained showed that the methodology used in our study was repeatable and reliable. The values established for the normal radial anatomical angles are relevant for future use as a reference for surgical treatment of angular deformities, malunions, non-unions, comminuted fractures, and future orthopaedic research.

Supplementary material for this paper is available online at http://dx.doi.org/10.3415/VCOT-16-02-0033.
Perivascular Cells in Diffuse Cutaneous Systemic Sclerosis Overexpress Activated ADAM12 and Are Involved in Myofibroblast Transdifferentiation and Development of Fibrosis
Authors: Cipriani Paola , Di Benedetto Paola , Ruscitti Piero , Liakouli Vasiliki , Berardicurti Onorina , Carubbi Francesco , Ciccia Francesco , Guggino Giuliana , Zazzeroni Francesca , Alesse Edoardo , Triolo Giovanni , Giacomelli Roberto
Published: June 2016

Objective.

Microvascular damage is pivotal in the pathogenesis of systemic sclerosis (SSc), preceding fibrosis, and whose trigger is not still fully understood. Perivascular progenitor cells, with profibrotic activity and function, are identified by the expression of the isoform 12 of ADAM (ADAM12) and this molecule may be upregulated by transforming growth factor-β (TGF-β). The goal of this work was to evaluate whether pericytes in the skin of patients with diffuse cutaneous SSc (dcSSc) expressed ADAM12, suggesting their potential contribution to the fibrotic process, and whether TGF-β might modulate this molecule.

Methods.

After ethical approval, mesenchymal stem cells (MSC) and fibroblasts (FB) were isolated from bone marrow and skin samples collected from 20 patients with dcSSc. ADAM12 expression was investigated in the skin and in isolated MSC and FB treated with TGF-β by immunofluorescence, quantitative real-time PCR, and western blot. Further, we silenced ADAM12 expression in both dcSSc-MSC and -FB to confirm the TGF-β modulation.

Results.

Pericytes and FB of dcSSc skin showed an increased expression of ADAM12 when compared with healthy control skin. TGF-β in vitro treatment induced a significant increase of ADAM12 in both SSc-MSC and -FB, with the higher levels observed in dcSSc cells. After ADAM12 silencing, the TGF-β ability to upregulate α-smooth muscle actin in both SSc-MSC and SSc-FB was inhibited.

Conclusion.

Our results suggest that in SSc, pericytes that transdifferentiate toward activated FB are present in the vascular tree, and TGF-β, while increasing ADAM12 expression, may modulate this transdifferentiation.
Visual Complications in Patients with Biopsy-proven Giant Cell Arteritis: A Population-based Study
Authors: Saleh Muna , Turesson Carl , Englund Martin , Merkel Peter A. , Mohammad Aladdin J.
Published: June 2016

Objective.

To study the clinical and laboratory characteristics of patients with biopsy-proven giant cell arteritis (GCA) with visual complications, and to evaluate the incidence rate of visual complications in GCA compared to the background population.

Methods.

Data from 840 patients with GCA in the county of Skåne, Sweden, diagnosed between 1997 and 2010, were used for this analysis. Cases with visual complications were identified from a diagnosis registry and confirmed by a review of medical records. The rate of visual complications in patients with GCA was compared with an age- and sex-matched reference population.

Results.

There were 85 patients (10%) who developed ≥ 1 visual complication after the onset of GCA. Of the patients, 18 (21%) developed unilateral or bilateral complete visual loss. The mean age at diagnosis was 78 years (± 7.3); 69% were women. Compared with patients without visual complications, those with visual complication had lower C-reactive protein levels at diagnosis and were less likely to have headache, fever, and palpable abnormal temporal artery. The use of β-adrenergic inhibitors was associated with visual complications. The incidence of visual complications among patients with GCA was 20.9/1000 person-years of followup compared to 6.9/1000 person-years in the reference population, resulting in a rate ratio of 3.0 (95% CI 2.3–3.8).

Conclusion.

Ten percent of patients with GCA developed visual complications, a rate substantially higher than that of the general population. Patients with GCA who had visual complications had lower inflammatory responses and were more likely to have been treated with β-adrenergic inhibitors compared with patients without visual complications.
De l’argent facile
Authors: Duport Claire
Published: June 2016

Activité illicite, souvent pratiqué par des personnes pour qui l’accès aux revenus légaux est devenu impossible, le trafic de drogue constitue une forme de redistribution des richesses. Toutefois, les recettes de cette économie lucrative sont loin de faire la prospérité de l’ensemble des acteur.trice.s impliqué.e.s dans ce commerce. Alors qu’une infime minorité à la tête des trafics bénéficie de l’essentiel des profits, les vendeurs de rue apparaissent comme des employés souvent exploités et particulièrement vulnérables.
Les « guerres de l’opium » : les canons de la liberté
Authors: Roux Alain
Published: June 2016

L’impérialisme occidental n’a pas toujours prétexté de son intention d’éradiquer les drogues pour justifier ses interventions hors de ses frontières. Par deux fois au cours du XIX e siècle, la Grande-Bretagne et les autres puissances sont au contraire intervenues pour contraindre la Chine à accepter d’être inondée d’opium, seule façon de rétablir leur balance commerciale lourdement déficitaire avec l’Empire du Milieu. Ces guerres de l’opium racontent une autre histoire de la mondialisation marchande : plutôt qu’une évolution pacifique dans l’intérêt mutuel des différentes parties, cette mondialisation se dévoile comme l’imposition violente de conditions d’échanges dissymétriques, c’est-à-dire d’un ordre arbitraire, de la loi du plus fort.
La politique de décriminalisation des drogues au Portugal
Authors: Landel Pauline , Zielinska Anna C. , Landel Pauline
Published: June 2016

Depuis quinze ans, le Portugal a dépénalisé l’usage de toutes les drogues. Le Professeur João Goulão a été l’un des acteurs essentiels de ce tournant législatif majeur. Dans cet entretien, il revient sur les raisons qui ont conduit les autorités portugaises à adopter une politique en matière de drogues à contre-courant des dogmes habituels.
Démocratie et néolibéralisme au Chili
Authors: Bataillon Gilles
Published: June 2016

Comment comprendre et caractériser l’expérience politique chilienne aujourd’hui à plus de quarante ans du putsch qui fit du Chili, non seulement le théâtre d’une dictature autoritaire mais aussi le premier laboratoire du projet néolibéral ? L’enquête menée par Kathya Araujo et Danilo Martuccelli, Desafíos comunes. Retrato de la sociedad chilena y sus individuos , rompt avec les lectures qui opposent l’emprise de la matrice néolibérale et la réinstauration d’un régime démocratique. Ils montrent comment les idéaux démocratiques sont devenus un principe organisateur du social à tous les niveaux. C’est désormais l’ensemble des acteurs et des institutions qui connaissent des tensions nouvelles qu’explore cette enquête magistrale.
Le nounours et l’enfant : la violence de l’idéalité
Authors: Chaudoye Guillemine , Riazuelo Hélène , Cupa Dominique
Published: June 2016

À partir d’une illustration clinique, ce travail propose une réflexion sur les notions d’idéal et de maladie d’idéalité chez un enfant de huit ans présentant des troubles du comportement. De nombreux personnages vont venir accompagner ce récit et étayer l’élaboration tranféro-contre-transférentielle de cette prise en charge psychothérapeutique. Nounours, le monstre, l’arbre-armoire sont autant de « héros » qui vont avoir des rôles essentiels dans le processus thérapeutique. En effet, le travail thérapeutique va peu à peu mener à l’utilisation, dans le transfert et le contre-transfert, de ces « héros » comme objets transitoires à celle de ces « héros » comme objets transitionnels, du côté de fonctions maternelles et paternelles constituantes, signant ainsi une sortie progressive de cette maladie d’idéalité.
Troubles de l’alimentation et destins de l’oralité en néonatologie
Authors: Pradillon Gaëlle , Berriex Annie
Published: June 2016

La question de l’oralité en néonatologie ne peut se résumer à « bien boire » ou « ne pas boire ». Au-delà des aspects neurophysiologiques du développement, l’observation clinique nous invite à porter une attention renouvelée aux enjeux psychiques de la succion et de la tétée pour les nouveau-nés prématurés et à interroger ce qui fait apparaître parfois des pathologies précoces et singulières de l’alimentation. Au travers de deux vignettes cliniques, seront abordés l’articulation entre la succion fœtale et néonatale et les procédés auto-calmants, les effets de la qualité du lien à la mère sur l’oralité du bébé prématuré et le déni de la sexualité infantile en néonatologie.