bluuebunny/crossref_metadata_2025 huggingface dataset:
VIBRATION ANALYSIS OF HEAVY WEAPONS IN TRANSIT BY AIRCRAFT IN FRACTAL SPACE CONSIDERING LOCATION DEVIATION
Authors: KANG YONG-GANG , KOU SHUAI-JIA , SONG SI-REN , CHANG YU-ZHEN , WANG AN-YANG , CHEN YONG-GANG
Published: January 2024

Air transportation constitutes a significant advancement in enhancing transportation efficiency. Nonetheless, when this modality is employed for the transit of large-scale armaments and equipment, the vibrational properties of these items within the aircraft’s cabin, coupled with potential deviations from their designated installation positions, emerge as critical factors that could compromise the safety of such transportation endeavors. To accommodate the unique environmental conditions of low-temperature and low-pressure prevalent in high-altitude air transportation, this model employs a fractal frequency formula for an expedited and accurate characterization of vibrational properties, while also providing a detailed analysis of the errors attributable to positional deviations in these vibration assessments. The findings of this research demonstrate that the computational accuracy achieved herein surpasses that of the variational iteration method (VIM) and the homotopy perturbation method (HPM). Moreover, the investigation into the damping effects of inertial forces within fractal dimensions unveils innovative prospects for optimizing nonlinear vibration systems under the challenging conditions of low-temperature and low-pressure environments.
HYPER-WIENER INDEX ON LEVEL-3 SIERPINSKI GASKET
Authors: XU JIAJUN , XI LIFENG
Published: January 2024

The hyper-Wiener index plays an important role in chemical graph theory. In this paper, using the technique named finite pattern, we discuss the hyper-Wiener index on level-3 Sierpinski gasket which is a self-similar fractal.
Development of the Short-Form Yin Deficiency Scale Using Three Item Reduction Approaches
Authors: Park Young-Jae , Ok Ji-Myung
Published: January 2024

Background. Yin deficiency (YD) is a pathological condition characterized by emaciation, afternoon fever, dry mouth, and night sweats. The incidence of YD is 23.3%. A 27-item Yin Deficiency Scale (YDS) was developed to estimate the clinical severity of YD. This study aimed to develop three short-form YDS versions to reduce the burden of response time, using three item-reduction approaches: Rasch, equidiscriminatory item-total correlation (EITC), and factor-based analyses. Methods. Two datasets were analyzed from previous studies (169 outpatients from May to June 2009 and 237 healthy college students from January to April 2016). The optimal response category was examined using Rasch analysis. Items with higher item-total correlations were determined using the EITC. Using a factor-based approach, the items were reduced, while maintaining the original YDS construct. Reliability was estimated using the person separation index (PSI) and Cronbach’s α values. The predictive accuracy was examined using the area under the curve (AUC). Finally, the relationship between YD and dysfunctional breathing (DB) was examined using factor scores from the YDS and the Korean version of the Nijmegen Questionnaire (KNQ). Results. We developed two 14-item YDS versions using the Rasch and EITC approaches, and a 16-item YDS version using a factor-based approach. Rasch analysis suggested an optimal response category of five points. The PSI of Rasch and Cronbach’s α of the EITC and factor-based versions were 2.19, 0.855, and 0.827. The AUCs of the three short-form YDS were 0.812, 0.811, and 0.818. The sensitivity of the EITC-YDS was 0.632, which was lower than its specificity of 0.875. The fatigue-related scores of the factor-based YDS were fairly correlated with the factor scores of the KNQ estimating the DB (r = 0.349–0.499). Conclusion. The 14-item Rasch- and 16-item factor-based YDS may replace the original YDS during YD’s primary screening, epidemiological surveys, and health checkups.
Desafíos de la Industria 4.0 en las PYMES
Authors: Geldes Cristian , Castillo-Vergara Mauricio , Muñoz-Cisterna Victor
Published: January 2024

En los últimos años se han desarrollado crecientemente una serie de nuevas tecnologías que están cambiando el mundo de los negocios. Este conjunto de nuevas tecnologías y sus aplicaciones se denominan “Industria 4.0”. Dado este escenario, este artículo plantea una serie de desafíos para que las PYMES se sumen a esta nueva tendencia. Continuar leyendo...
Table S1 from Dietary Folate and Cofactors Accelerate Age-dependent p16 Epimutation to Promote Intestinal Tumorigenesis
Authors: Yang Li , Peery Robert C. , Farmer Leah M. , Gao Xia , Zhang Yiqun , Creighton Chad J. , Zhang Lanjing , Shen Lanlan
Published: January 2024

Supplementary Table S1 shows comparison of diets between current and previous studies.
Figure S4 from Dietary Folate and Cofactors Accelerate Age-dependent p16 Epimutation to Promote Intestinal Tumorigenesis
Authors: Yang Li , Peery Robert C. , Farmer Leah M. , Gao Xia , Zhang Yiqun , Creighton Chad J. , Zhang Lanjing , Shen Lanlan
Published: January 2024

Supplementary Figure S4 shows scRNA-seq analysis which reveals the immune landscape of colon tumors from supplemented mice.
Figure S3 from Dietary Folate and Cofactors Accelerate Age-dependent p16 Epimutation to Promote Intestinal Tumorigenesis
Authors: Yang Li , Peery Robert C. , Farmer Leah M. , Gao Xia , Zhang Yiqun , Creighton Chad J. , Zhang Lanjing , Shen Lanlan
Published: January 2024

Supplementary Figure S3 shows dietary methyl donor supplementation markedly increases tumor cell proliferation and immune cell infiltration.
Figure S2 from Dietary Folate and Cofactors Accelerate Age-dependent p16 Epimutation to Promote Intestinal Tumorigenesis
Authors: Yang Li , Peery Robert C. , Farmer Leah M. , Gao Xia , Zhang Yiqun , Creighton Chad J. , Zhang Lanjing , Shen Lanlan
Published: January 2024

Supplementary Figure S2 shows an enhanced 1C metabolic pathway that contributes to tumor growth in response to dietary methyl donor supplementation.
Figure S1 from Dietary Folate and Cofactors Accelerate Age-dependent p16 Epimutation to Promote Intestinal Tumorigenesis
Authors: Yang Li , Peery Robert C. , Farmer Leah M. , Gao Xia , Zhang Yiqun , Creighton Chad J. , Zhang Lanjing , Shen Lanlan
Published: January 2024

Supplementary Figure S1 shows that colon tumors from mice fed with control diet had significantly smaller size.
Data from Dietary Folate and Cofactors Accelerate Age-dependent p16 Epimutation to Promote Intestinal Tumorigenesis
Authors: Yang Li , Peery Robert C. , Farmer Leah M. , Gao Xia , Zhang Yiqun , Creighton Chad J. , Zhang Lanjing , Shen Lanlan
Published: January 2024

Abstract
The extent to which non-genetic environmental factors, such as diet, contribute to carcinogenesis has been long debated. One potential mechanism for the effects of environmental factors is through epigenetic modifications that affect gene expression without changing the underlying DNA sequence. However, the functional cooperation between dietary factors and cancer-causing epigenetic regulation is largely unknown. Here, we use a mouse model of age-dependent p16 epimutation, in which the p16 gene activity is directly controlled by promoter DNA methylation. We show p16 epimutation is modulated by folate and cofactors in dietary supplementation, which leads to increased colon cancer risk. Importantly, our findings provide functional evidence concerning the safety of folate fortification in the general population.
Significance:
Our study demonstrates that dietary folate and cofactors modulate tumor-suppressor gene methylation to increase intestinal tumorigenesis. Our findings highlight the need for monitoring the long-term safety of folate fortification in high-risk individuals.
Long range based effective field monitoring system
Authors: Rajani Kumari Popuri , Suneetha Chalasani , Sri Lakshmi Vadlamudi , Rama Priya Nakka , Venkata Rajanna Bodapati , Sudhakar Ambarapu
Published: January 2024

Adoption of the internet of things (IoT) is moving forward quickly because of the developments in communication protocols and technology involving sensors. The IoT is promoting real-time agricultural field monitoring from any distant place. For the IoT to be implemented effectively there are a number of agricultural issues related to less power usage and long-distance transfer of data are to be addressed. By using LoRa, which is a wireless communication system for IoT applications, these difficulties can be avoided when sending information from fields of crops to a web server. Acustomized sensor node and LoRa are used in this work to transmit continuously updated information to a remote server. Monitoring the quality of water, and reducing wasteful use of water are the main goals.
Cannes 1964: marco do engajamento dos críticos franceses ao Cinema Novo
Authors: Figueiró Belisa
Published: January 2024

Este artigo examina como o Festival de Cannes de 1964 foi decisivo para que a crítica francesa começasse a reverenciar o Cinema Novo em um momento em que algumas publicações, especialmente a Cahiers du Cinéma, passavam por uma reformulação político-ideológica. Por meio de uma análise histórica sobre a chegada dos cinemanovistas à Cannes, da avaliação dos jornais e revistas publicados durante o festival – encontrados nos arquivos da Cinemateca Francesa –, e de uma entrevista com a crítica Sylvie Pierre, foi possível verificar que Cannes 1964 marcou o início de um alinhamento entre os franceses e os cinemanovistas, e quais eram os interesses que estavam em jogo em cada um dos lados.
Mirando montagens e montando miragens em fotorreportagem: campos de concentração na Folha de S.Paulo
Authors: Macêdo Daniel
Published: January 2024

O ano de 2019 foi marcado pela circulação de diferentes produções que materializam perspectivas ao narrar os campos de concentração experienciados em 1932. A Folha de S.Paulo participa da trama publicando matéria na edição impressa, articulada com fotorreportagem em versão digital. Com atenção à narrativa visual, este trabalho é resultado de um experimento metodológico, tomando atos de leitura para mirar montagens e para montar miragens como operadores pelos quais tensionamentos entre leitor, texto e contexto emergem, desestabilizando os contornos simbólicos e temporais que amparam acontecimentos em distintas textualizações.
Ação política através da imagem no conflito palestino-israelense: resenha de Screen Shots de Rebecca L. Stein
Authors: Baldin Vitoria Paschoal
Published: January 2024

Resultado de anos de pesquisa sobre as relações entre a imagem técnica e a ação política,  Screen Shots de Rebecca L. Stein reflete sobre as promessas da câmera como ferramenta de ativismo tensionadas a partir dos desafios enfrentados contemporaneamente. Com a disseminação global de smartphones, as câmeras se proliferaram como ferramentas políticas entre diversos atores nos Territórios Palestinos, como ativistas palestinos, soldados israelenses, colonos e defensores dos direitos humanos. A autora aborda como esses sujeitos depositaram profunda esperança nessas tecnologias como ferramenta para atingir suas respectivas agendas políticas. Stein investiga tais sonhos e a desilusão subsequente.
Entrevista com Giuliana Bruno
Authors: Delfino Luiza Wollinger , Hamburger Esther Império , Mello Cecilia , Bruno Giuliana
Published: January 2024

Em antecipação à sua visita a São Paulo em dezembro de 2023, a Significação: Revista de Cultura Audiovisual realizou uma entrevista online de duas horas com Giuliana Bruno, com foco em Atmospheres of Projection e em suas outras contribuições inovadoras. Bruno é palestrante convidada do workshop interdisciplinar organizado pela Fundação de Amparo à Pesquisa do Estado de São Paulo (Fapesp) em dezembro de 2023. Antes disso, ministrará uma Masterclass na Universidade de São Paulo (USP), evento promovido pelo Laboratório de Investigação e Crítica Audiovisual (Laica) e pela Significação: Revista de Cultura Audiovisual, com o apoio da Fapesp, do Instituto de Estudos Avançados (IEA-USP), do Programa de Pós-Graduação em Meios e Processos Audiovisuais da Escola de Comunicações e Artes (PPGMPA-ECA) e do Programa de Pós-Graduação e m Multimeios da Universidade Estadual de Campinas (PPGM-Unicamp).
Casamento por convergência: identidades estatais e a aliança entre Síria e Irã
Authors: Munareto Camila Hirt , Silva Gabriela Santos da
Published: January 2024

Este artigo procura compreender como as identidades inf luenciam o surgimento e a manutenção da aliança sírio iraniana. Por meio de uma análise qualitativa e interpretativa da literatura sobre o tema, a pesquisa investiga três momentos: a guerra entre Irã e Iraque, a Guerra do Líbano e o conflito na Síria, a fim de compreender os elementos que constituem esse vínculo. Argumenta-se que a aproximação – e a manutenção – da aliança entre Síria e Irã é pautada pela complementariedade de suas identidades estatais, constituindo o que denominamos de “casamento por convergência”.
DA CASA PARA A RUA
Authors: Nassar Paulo , Farias Luiz Alberto de , Andreucci Ana Claudia Pompeu Torezan , Curcio Gustavo Orlando Fudaba , Aiello Thaís
Published: January 2024

O entrevistado desta primeira edição da Revista Interfaces da Comunicação é Roberto DaMatta, historiador, especialista em Antropologia Social e que tornou-se mestre e Ph.D em, respectivamente, 1969 e 1971 pelo Peabody Museum da Universidade de Harvard. Foi Chefe do Dept. de Antropologia do Museu Nacional e coordenador do seu Programa de Pós-Graduação em Antropologia Social (de 1972 a 1976). É professor emérito da Universidade de Notre Dame, USA, onde ocupou a Cátedra Rev. Edmund Joyce, c.s.c., de Antropologia de 1987 a 2004. Atualmente, é professor titular da Pontifícia Universidade Católica do Rio de Janeiro. Realizou pesquisas etnológicas entre os índios Gaviões e Apinajés. Foi pioneiro nos estudos de rituais e festivais em sociedades industriais, tendo investigado o Brasil como sociedade e sistema cultural por meio do carnaval, do futebol, da música, da comida, da cidadania, da mulher, da morte, do jogo do bicho e das categorias de tempo e espaço.
A Comunicação e suas novas narrativas: desafios e oportunidades no acesso aos serviços emergenciais 190 pelas pessoas com deficiência auditiva ou surdez
Authors: Barreto Ricardo Souza
Published: January 2024

O presente artigo, fruto do estudo sobre o tema de novas narrativas da comunicação no programa de Pós-Doutorado junto ao Departamento de Relações Públicas, Propaganda e Turismo da Escola de Comunicações e Artes da Universidade de São Paulo, tem por objetivo ofertar uma reflexão sobre as barreiras e sobre a evolução tecnológica e social do emprego de novas narrativas de comunicação no ambiente que envolve pessoas com deficiência (PcD) auditiva ou surdez que utilizam o serviço de emergência 190 no Estado de São Paulo. A metodologia do estudo se baseia na pesquisa bibliográfica em livros, artigos científicos e indicadores de órgãos técnicos e marcos regulatórios. Nesse contexto, são abordados os processos e as dimensões da comunicação de emergência 190 na maior cidade da América Latina, perpassando pelas telecomunicações e a operacionalização do Centro de Operações da Polícia Militar do Estado de São Paulo, pelas regulamentações que afiançam o direito à comunicação como parte dos direitos fundamentais do ser humano e da isonomia no acesso aos serviços públicos, bem como pelo Projeto E-SMS, pioneiro no Brasil, que promoveu a comunicação de emergência de PcD auditiva ou surdez, sem intermediação de outras pessoas ou órgãos, com os serviços de atendimento telefônico emergencial. Como conclusão, evidencia-se que o serviço E-SMS é um exemplo de que a comunicação guiada por suas novas narrativas detém a capacidade de aperfeiçoar a inclusão social e a erradicação de todas as formas de discriminação por motivo de deficiência, raça, religião e gênero.
Visibilidade, influência e consumo: o processo de formação da opinião pública de marcas por meio das redes de hiperconexão digital
Authors: Zimmermann Daniel Dubosselard , Trindade Eneus , Meira Karla de Melo Alves
Published: January 2024

Este artigo aborda a representação das organizações nas redes de hiperconexão digital, considerando as concepções sociais em seu caráter ativo, tanto nas mentes das pessoas quanto nos meios de comunicação. Para tanto, fundamenta-se em levantamento bibliográfico e análise múltipla de casos para apresentar, por meio de um caminho teórico baseado nos conceitos de visibilidade (SILVA; BARICHELLO, 2006) e hiperconexão (TERRA, 2019), que reflete a influência da publicidade de marcas na formação da agenda pública por meio da interação com a agenda das mídias sociais digitais (ZIMMERMANN, 2022). Com base em autores como Thompson (2008, 2018), Keen (2012), Stasiak (2014), Éric Sadin (2015), Lindstrom (2016), Dominique Vinck (2016), Loh (2019), Sodré (2021), tangencia o espectro do big data ao small data, na perspectiva dos algoritmos como gatewatching e gatekeeping,e problematiza os quadros narrativos pautados pela publicidade como sendo framing e priming, propostos como segundo nível do agenda-setting. Nesse cenário, em que os algoritmos são responsáveis por dirigir as mensagens para públicos específicos, a publicidade de marca, para entrar na agenda, precisa estar alinhada em seu discurso e sua prática. Por conclusão, este artigo atenta ao pensar perspectivas futuras, sem negligenciar os presságios do metaverso, em que são colocadas a publicidade de marcas e o seu agendamento digital.
Falar às memórias
Authors: Nassar Paulo , Farias Luiz Alberto de
Published: January 2024

Apresentação/Editorial da 2ª edição da Revista Interfaces da Comunicação.
Os lugares de memória são lugares extraordinários
Authors: Nassar Paulo
Published: January 2024

Experiência/relato da edição.
Após o centenário da Semana de Arte Moderna, ainda há um movimento: o meme como manifestação cultural
Authors: Sgrignolli Ruth Carolina Rodrigues , Junqueira Michelle Asato
Published: January 2024

O trabalho parte da premissa da transformação operada na arte pela Semana de Arte Moderna de 1922 para trazer o paralelo com a manifestação cultural digital dos “memes” e como estes instrumentos podem ser também forma de divulgação da arte, da literatura e da política. Construído pelo método indutivo, é composto por três partes, abordando a arte como forma de manifestação humana e política para, então, abordar casos em que esta nova narrativa foi objeto de contestação e “viralização” no ambiente virtual.
Identidade cultural e inteligência artificial: uma análise das imagens de festas populares brasileiras geradas pela plataforma Midjourney
Authors: Silva Diogo Cortiz da , Nobre Leonardo Braga , Ladeia Tatiana Amaral Silva , Viegas Luís Eduardo Alonso
Published: January 2024

A inteligência artificial tem desempenhado um papel de mudança significativa na sociedade atual. O objetivo deste trabalho é analisar se os sistemas de inteligência artificial generativa, em especial o Midjourney, capturam e recriam, de forma autêntica, as representações culturais. A metodologia adotada foi a semiótica de Peirce, a escolha se deu porque a semiótica pode auxiliar nos mecanismos de construção dos significados. Os resultados obtidos mostram que os sistemas de inteligência artificial generativa ainda não conseguem, quando se trata de representação cultural, ser fiéis aos elementos da identidade cultural. Dessa forma, conclui-se que os sistemas, ao gerarem conteúdo digital sobre as festividades pesquisadas, não conseguem eliminar o viés e acabam construindo imagens que não as representam.
Likelihood-free posterior estimation and uncertainty quantification for diffusion MRI models
Authors: Karimi Hazhar Sufi , Pal Arghya , Ning Lipeng , Rathi Yogesh
Published: January 2024

Diffusion magnetic resonance imaging (dMRI) allows to estimate brain tissue microstructure as well as the connectivity of the white matter (known as tractography). Accurate estimation of the model parameters (by solving the inverse problem) is thus very important to infer the underlying biophysical tissue properties and fiber orientations. Although there has been extensive research on this topic with a myriad of dMRI models, most models use standard nonlinear optimization techniques and only provide an estimate of the model parameters without any information (quantification) about uncertainty in their estimation. Further, the effect of this uncertainty on the estimation of the derived dMRI microstructural measures downstream (e.g., fractional anisotropy) is often unknown and is rarely estimated. To address this issue, we first design a new deep-learning algorithm to identify the number of crossing fibers in each voxel. Then, at each voxel, we propose a robust likelihood-free deep learning method to estimate not only the mean estimate of the parameters of a multi-fiber dMRI model (e.g., the biexponential model), but also its full posterior distribution. The posterior distribution is then used to estimate the uncertainty in the model parameters as well as the derived measures. We perform several synthetic and in-vivo quantitative experiments to demonstrate the robustness of our approach for different noise levels and out-of-distribution test samples. Besides, our approach is computationally fast and requires an order of magnitude less time than standard nonlinear fitting techniques. The proposed method demonstrates much lower error (compared to existing methods) in estimating several metrics, including number of fibers in a voxel, fiber orientation, and tensor eigenvalues. The proposed methodology is quite general and can be used for the estimation of the parameters from any other dMRI model.
Investigation and Tranquillity in Sextus Empiricus
Authors: Svavarsson Svavar Hrafn
Published: January 2024

Sextus Empiricus tells us that the sceptics conduct investigations. Genuine investigations are commonly taken to have some connection with the aim of discovering truth. Sextus also tells us that the sceptics aim at tranquillity in their investigations. But they do this only by suspending judgement. In what sense, then, are their investigations connected with the aim of discovering truth? This article argues that there is a sense in which Sextus can reasonably take sceptical investigations to be connected with the aim of discovering truth, even though the sceptics only aim at preserving their tranquillity by suspending judgement.
Combination of Focused Ultra Sound and MnO2/GOD Loaded Microbubble Nanoparticles in Targeted Tumor Therapy of Breast Cancer
Authors: He Meiqing , Li Yanchuan , Abdi Abyaneh Fahimeh , Mohseni Melerdi Faezeh , Jabbari Hadi , Arabinour Mahdi
Published: January 2024

In order to increase the absorption of anticancer medications into malignancies in a noninvasive and targeted way, focused ultrasound in conjunction with microbubble (MB) might improve drug delivery. Here, we test two exemplary focused ultrasound-driven drug delivery enhancement approaches
(MB and Doxorubicin-coated nanoparticle complex using MnO2/GOD) and confirm their acute drug delivery augmentation. Experiments were performed utilizing the MDA-MB-231 breast cancer cell line in an in vivo animal model. We observed that MB containing Doxorubicin-coated nanoparticle
using MnO2/GOD complex increased Doxorubicin concentrations in tumors. These findings suggest that MB and MB nanoparticle based anticancer drug delivery may considerably enhance the efficacy of anticancer medications given to tumors in a short period of time using low-intensity
focused ultrasound.
Inhibitory Effect of Iron Oxide Nanoparticle Liposome on the Growth of Non-Small Cell Lung Cancer In Vitro
Authors: Zhou Yong , Yu Ao , Zhao Gefei , Chen Baojun
Published: January 2024

In this study, we assessed the inhibitory potential of magnetic liposomes as gene vectors targeting human non-small cell lung cancer (NSCLC) growth In Vitro via hairpin RNA (shRNA) under the influence of an external magnetic field. Specifically, we engineered the plasmid pGFPshIGF-1R
to co-express green fluorescent protein (GFP) and shRNA against insulin-like growth factor-1 receptor (IGF-1R), a gene associated with NSCLC progression. Utilizing superparamagnetic iron oxide nanoparticles (CombiMAG) and Lipofectamine2000,we constructed magnetic liposomes to deliver pGFPshIGF-1R
into A549 NSCLC cells. Varying magnetic field strengths and exposure times were employed. The efficacy of transfection was gauged against liposome-only methods using Western blot analysis to measure IGF-1R protein reduction. In Vitro, the magnetic approach exhibited higher transfection
efficiency (66.3%) compared to liposomes alone (22.1%), marking a threefold enhancement. In vivo, using a subcutaneous A549 tumor model in nude mice, shRNA-transfected A549 cells displayed substantial IGF-1R protein inhibition (interference rates: 56.1% and 85.1%). Importantly, magnetic
transfection outperformed liposomal transfection in suppressing IGF-1R protein expression (P <0.05). Our findings suggest that magnetic liposome-mediated gene delivery, facilitated by an external magnetic field, holds promise as a targeted and safe therapeutic strategy for lung cancer.
Magnetic Double Hydrogen Artesunate Nano Liposome on CAL Tongue Squamous Carcinoma and Laryngeal Hep-2-27 Cell Lines: An In Vitro Study
Authors: Dong Jiadi , Chen Jingjing , Li Qun , Qiu Shijie
Published: January 2024

With the continuous development of nanomedicine, scholars have reported many studies on the application of nanocarriers to improve the efficiency of tumor diagnosis and treatment. We designed magnetic nanoparticles combined with liposomes to carry DHA (DHA-MAG-NL) nanoparticles to improve
the biocompatibility of DHA, protect DHA activity, and increase DHA blood concentration. To study the inhibitory effect of magnetic dihydroartemisinin nano-liposomes on the proliferation of human tongue squamous cell carcinoma CAL-27 cell line and laryngeal carcinoma Hep-2 cell line In
Vitro. The effect of DHA-Mag-NL concentration gradient on the proliferation of Hep-2 and Cal-27 cells at different intervention times was detected by MTT assay. The effects of DHA concentration of 40 μM on the cell cycle and apoptosis of Hep-2 and Cal-27 were analyzed by flow
cytometry. Statistical analysis showed that DHA-Mag-NL had a weak inhibitory effect on Cal-27 cells. The apoptosis-promoting effect of DHA-Mag-NL on Cal-27 tumor cells was weaker than that of DHA for 24 h. We concluded that DHA-Mag-NL can inhibit the proliferation of Hep-2 and Cal-27 tumor
cells and promote apoptosis, which is dependent on drug dose and intervention time.
Angiopoietin-Like Protein 2 Serves as an Independent Prognostic Biomarker and Correlates with Immune Infiltration in Osteosarcoma
Authors: Zhou Huan , Chen Guang
Published: January 2024

Osteosarcoma mostly occurs in adolescents, with high disability and mortality rates and poor prognosis. Angiopoietin-like protein 2 (ANGPTL2) is a secreted glycoprotein protein, which plays important roles in cellular processes and various diseases. Microarray gene expression data GSE16088
and GSE36001 were downloaded from Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo). The Complex Heatmap package was used to construct heatmaps, the gglot2 R language package to plot
volcanoes and differential expression data, and the “VennDiagram” R language package to plot Wayne plots. Western booting and qRT-PCR was used to detect ANGPTL2 expression in osteosarcoma tissues. proteins. In addition, the GeneMANIA database (http://www.genemania.org)
was cited to predict the ANGPTL2 interaction network. We used the single-sample gene-set enrichment analysis (ss GSEA) algorithm to quantify the immune infiltration of osteosarcoma. In the GSE16088 database there were 5005 that met the selected threshold of |log2(FC)| > 1 and P <0.05,
of which 2719 were highly expressed and 2286 were lowly expressed. WB and qRT-PCR results showed that ANGPTL2 was significantly up-regulated in osteosarcoma tissues. ssGSEA showed that high ANGPTL2 expression in osteosarcoma patients had a worse prognosis. ssGSEA showed that high ANGPTL2 expression
was correlated with Mast cells and Th1 cells. Collectively, ANGPTL2 can be an independent prognostic biomarker and correlated with immune infiltration in osteosarcoma.
Long Noncoding RNA HOXC-AS2 Inhibits Cell Proliferation in Colorectal Cancer by Regulating UBE2L6 Expression Through Sponging miR-146b-5p
Authors: Chen Shihao , Wang Xin , Niu Mengye , Shang Fangjian , Liu Ying , Guo Shang , Zheng Qian , Li Dongyun , Su Xingkai , Zhao Zengren , Jiang Xia
Published: January 2024

It has been demonstrated that lncRNA HOXC-AS2 regulates the tumorigenesis and progression of multiple tumors. In present study we explored the biological function of HOXC-AS2 in colorectal cancer and its potential mechanisms. Firstly, we found that the expression of HOXC-AS2 was down-regulated
in colorectal cancer tissues than its adjacent normal tissues, and the expression level of HOXC-AS2 was found to show an inversely correlated with tumor size by analyzing the clinicopathological characteristics. HOXC-AS2 knockdown significantly increased its proliferation and colony formation
ability in colorectal cancer cells, and conversely, HOXC-AS2 overexpression significantly decreased its proliferation and colony formation ability, suggesting that HOXC-AS2 exerts anti-tumor effect in colorectal cancer. In addition, we found that miR-146b-5p, as a downstream bioactive factor,
could be targeting sponged by HOXC-AS2 in colorectal cancer, and the expression of UBE2L6 was regulated by miR-146b-5p. Thus, HOXC-AS2 inhibits the proliferation and colony formation ability in colorectal cancer cells by targeted sponging miR-146b-5p and then regulates UBE2L6.
Protective Effect of Overexpression of Uqcrc1 in H2O2-Induced Myocardial Injury
Authors: Jin Hongrui , Li Yuzhou , Liu Hengdao , Xiao Yunfei , Zhang Yong , Cheng Jingliang
Published: January 2024

This study investigated the role of Uqcrc1 in oxidative stress and apoptosis induced by hydrogen peroxide (H2O2) in cardiomyocytes. H9c2 cells were divided into four groups: control, H2O2-treated, Lv-NC+H2O2 (negative
control lentivirusinfected with H2O2), and Lv-Uqcrc1+H2O2 (Uqcrc1 overexpressed lentivirus-infected with H2O2 for 12 hours). Uqcrc1 expression was quantified using qRT-PCR and Western blotting. Cell proliferation was assessed
with CCK-8, and various oxidative stress markers (LDH, MDA, SOD, CAT, GSH-Px) were measured. Apoptosis was evaluated using Tunel assays, intracellular ROS levels, and Cleaved Caspase3 and Caspase9 expression. Additionally, the impact on the Wnt/β-catenin pathway was examined. In
the Lv-Uqcrc1 group, Uqcrc1 expression was notably higher. H2O2 treatment reduced cell proliferation and increased LDH leakage, MDA, ROS levels, and apoptosis. Cleaved Caspase3 and Caspase9 expression increased, indicating apoptosis. Lv-Uqcrc1+H2O2
group exhibited improved cell proliferation, decreased LDH leakage, increased CAT, SOD, GSH-Px activities, reduced MDA and ROS levels, and reduced apoptosis with lower Cleaved Caspase3 and Caspase9 expression. Uqcrc1 overexpression also inhibited Wnt/β-catenin pathway overactivation.
In oxidative stress conditions, Uqcrc1 protected against myocardial cell injury, reducing apoptosis and oxidative damage. This study highlights Uqcrc1’s significance in cardiovascular diseases.
Retinal Ganglion Cell Apoptosis via Downregulating Survivin by Protocadherin 7 Overexpression
Authors: Dai Hanjun , Zhong Yanyan , Chen Hui , Lu Xiaohe
Published: January 2024

Glaucoma is a group of diseases characterized by progressive optic nerve degeneration that results in visual field loss and irreversible blindness. Growing evidences show that retinal ganglion cells (RGCs) apoptosis is an important pathological hallmark of glaucoma. In recent years,
protocadherin 7 (Pcdh7) has been recognized as a transmembrane protein that is broadly existed in the nervous system and crucial to the neuronal development. The results of this study represented that upregulating expression of Pcdh7 activates the intrinsic apoptotic pathway in RGCs. However,
overexpressing survivin inhibited RGCs apoptosis resulted from upregulation of Pcdh7. According to these data, we can infer that Pcdh7-survivin signaling cascade plays a vital role in RGC apoptosis, and it indicates a promising way to the therapeutic modalities of Glaucoma.
An Exploratory Content Analysis of Two Local Television Stations’ Tornado Warning Broadcasts
Authors: Sherman-Morris Kathleen , Ali S. M. Asger
Published: January 2024

In-depth analysis of the content of broadcast tornado warning coverage is limited. Such analysis is important due to local television’s role as a key source for tornado warning information. This study attempts to fill gaps in our knowledge regarding broadcast coverage of tornado warnings by demonstrating how local television news stations’ coverage of tornadic events can be systematically analyzed to better understand this element of warning communication. We reviewed both visual and verbal content for information such as the prominence of specific radar products, the geographic scale of warning communication, and common themes in verbal communication. A combination of deductive and inductive coding approaches was used to summarize the verbal content of the broadcasts. We found that the stations heavily used radar products with reflectivity and velocity surpassing correlation coefficient. The geographic scale of mapped products (street, city/county, and state level) appeared to be related to the rural or urban nature of the area warned, which may have implications for how readily rural residents would be able to personalize tornado threats. Verbal content was very similar between the two stations. The theme of monitoring and updating conditions, which included processes such as zooming in and out, making adjustments, reinforcing conditions, and providing damage reports was the most frequent communication type, likely because weathercasters use these processes to both communicate the warning and also to help themselves understand the situation. The results can inform future studies examining the influence of specific elements of broadcast warning coverage on risk perception and protective actions.

Significance Statement

Television is a key source for receiving or confirming tornado warnings, but few studies have examined the content of broadcast warnings in depth. This study examined the visual and verbal content of broadcast tornado warnings on two local television stations. Radar products were used heavily, and street-level coverage was more common when a tornado affected a metropolitan area. Coverage was most common at the city/county level. Verbal content included many elements of effective warning communication but at times included jargon that may not be understood by viewers. The results can serve as a springboard for future research on the impacts of these elements on risk perception and response. It can also serve future research by distinguishing what viewers of severe weather broadcasts are exposed to that nonviewers are not.
Managing Change: Love or Hate Relationships
Authors: Rana Geeta , Sharma Ravindra
Published: January 2024

The purpose of this article is to provide an overview of the latest developments in change management worldwide and to identify practical implications arising from leading-edge research. The methodology for this briefing involves an independent writer adding impartial comments and contextualising the articles. The article examines the importance of having a high-quality management team in evaluating, designing and executing successful change strategies; specifically, the team’s capability to structure the organisation in a way that is responsive and progressive is crucial for successful change management. The article also discusses creative problem-solving and its limitations, and indicates that an independent facilitator could promote creative problem-solving. The authors opine that business leaders must have a better understanding of the different roles they can assume to make sure that initiatives are sustainable and successful. Successful change leaders attribute their success to leading change rather than managing it. This article offers deep insights into newest developments in change management and underscores the importance of effective leadership in achieving successful change initiatives.
Investigating the Determinants of the Innovation Adoption for Continuous Improvement in a Teacher-Training Institution: Evidence from the COVID-19 Period
Authors: Satish Vidhya , Kaul Arshia , Kaul Vasundhara
Published: January 2024

We are living in an ever-changing world. The changes in science, technology and economics constantly impact areas closely related to human life, such as education and health. Organisations must rely on innovation to remain relevant and effective in a constantly evolving society. Educational institutions, namely schools, colleges or even training institutions, contribute to product innovation when they produce new and significantly different products and services such as new syllabi, new resources or new educational experiences like e-learning. They carry out process innovation when they bring about significant changes in how they provide their service, for instance, changes in the way their teachers work together, communicate with parents or offer services in collaboration with other institutions. With the onset of the medical catastrophe of COVID-19 and the pandemic thereafter, the education sector plunged itself into adapting to the use of technology to keep its service functional, albeit with significant challenges faced in its effective implementation. In this article, the focus is on studying technology implementation in an early childhood care teacher-training institute to establish service innovation in education in the post-pandemic era. Multiple perspectives were derived from all the stakeholders, such as student-teachers, teacher educators and heads of schools (as future employers) regarding teacher preparedness and student-teacher readiness. The inputs helped us to develop the conceptual model for future teaching-learning. The conceptual model A.D.O.P.T. (Act with growth mindset, Deliver Quality, Open to Adaptation, Prioritize with Precision, Team Engagement) highlights how the stakeholders move from makeshift ‘jugaad’ innovation to sustainable development of the educational system through grassroots innovation.
Calcitonin gene-related peptide antagonists in pregnancy: a disproportionality analysis in VigiBase®
Authors: Noseda Roberta , Bedussi Francesca , Gobbi Claudio , Ceschi Alessandro , Zecca Chiara
Published: January 2024

Background

Current evidence on the safety of calcitonin gene–related peptide antagonists (CGRP-A) in pregnancy for the treatment of both episodic and chronic migraine is scarce and does not yet provide definitive information. By querying VigiBase®, the World Health Organization global pharmacovigilance database, this study aimed to detect differences in the reporting frequency between CGRP-A and triptans in relation to pregnancy.

Methods

Disproportionality analyses on de-duplicated safety reports collected in VigiBase® as of 31.05.2023 reporting exposure to CGRP-A in pregnancy with or without pregnancy outcomes. A Reporting Odds Ratio (ROR) with a 95% confidence interval (CI) was used as a measure of disproportionality and the threshold for the detection of a signal of disproportionate reporting was set with a 95% CI lower limit > 1.

Findings

Four hundred sixty-seven safety reports reported exposure to CGRP-A in pregnancy, mostly originating from the United States of America (360/467, 77%), more frequently reported by patients (225/467, 48%), who were mainly females (431/467, 92%), and more frequently reported exposure to CGRP-A during pregnancy (400/467, 86%). Compared to triptans, no signals of disproportionate reporting were detected with CGRP-A either for the overall reporting of pregnancy-related safety reports (ROR 0.91, 95% CI 0.78–1.06), for the reporting of pregnancy outcomes (maternal and/or foetal/neonatal, ROR 0.54, 95% CI 0.45–0.66), or for the reporting of foetal/neonatal outcomes (ROR 0.53, 95% CI 0.41–0.68).

Conclusions

This study showed that, to date, there are no signals of increased reporting with CGRP-A compared to triptans in relation to pregnancy in VigiBase®. Future pharmacovigilance studies are needed to confirm these findings.
Artificial light at night reduces earthworm activity but increases growth of invasive ragweed
Authors: Mittmannsgruber Marion , Kavassilas Zenia , Spangl Bernhard , Gruber Edith , Jagg Elias , Zaller Johann G.
Published: January 2024

Background

Artificial light at night, also referred to as light pollution (LP), has been shown to affect many organisms. However, little is known about the extent to which ecological interactions between earthworms and plants are altered by LP. We investigated the effects of LP on anecic earthworms (Lumbricus terrestris) that come to the surface at night to forage and mate, and on the germination and growth of the invasive and allergenic ragweed (Ambrosia artemisiifolia). In a full factorial pot experiment in the greenhouse, we tested four factors and their interactions: LP (5 lux vs. 0 lux at night), earthworms (two individuals vs. none), plant species (seeding of ragweed only vs. mixed with Phacelia seeds) and sowing depth (seed placed at the surface vs. in 5 cm depth). Data were analysed using Generalized Linear (Mixed) Models and multifactorial ANOVAs with soil parameters as covariates.

Results

Light pollution reduced earthworm surface activity by 76% as measured by casting activity and toothpick index; 85% of mating earthworms were observed in the absence of LP. Light pollution in interaction with earthworms reduced ragweed germination by 33%. However, LP increased ragweed height growth by 104%. Earthworms reduced ragweed germination especially when seeds were placed on the soil surface, suggesting seed consumption by earthworms.

Conclusions

Our data suggest that anecic earthworms are negatively affected by LP because reduced surface activity limits their ability to forage and mate. The extent to which earthworm-induced ecosystem services or community interactions are also affected by LP remains to be investigated. If the increased height growth of ragweed leads to increased pollen and seed production, it is likely that the competition of ragweed with field crops and the risks to human health will also increase under LP.
Evaluation of a modified ultrasound-assisted technique for mid-thoracic epidural placement: a prospective observational study
Authors: Huang Chanyan , Chen Ying , Kou Mengjia , Wang Xuan , Luo Wei , Zhang Yuanjia , Guo Yuting , Huang Xiongqing , Meng Lingzhong , Xiao Ying
Published: January 2024

Background

Although mid-thoracic epidural analgesia benefits patients undergoing major surgery, technical difficulties often discourage its use. Improvements in technology are warranted to improve the success rate on first pass and patient comfort. The previously reported ultrasound-assisted technique using a generic needle insertion site failed to demonstrate superiority over conventional landmark techniques. A stratified needle insertion site based on sonoanatomic features may improve the technique.

Methods

Patients who presented for elective abdominal or thoracic surgery requesting thoracic epidural analgesia for postoperative pain control were included in this observational study. A modified ultrasound-assisted technique using a stratified needle insertion site based on ultrasound images was adopted. The number of needle passes, needle skin punctures, procedure time, overall success rate, and incidence of procedure complications were recorded.

Results

One hundred and twenty-eight subjects were included. The first-pass success and overall success rates were 75% (96/128) and 98% (126/128), respectively. In 95% (122/128) of patients, only one needle skin puncture was needed to access the epidural space. The median [IQR] time needed from needle insertion to access the epidural space was 59 [47–122] seconds. No complications were observed during the procedure.

Conclusions

This modified ultrasound-assisted mid-thoracic epidural technique has the potential to improve success rates and reduce the needling time. The data shown in our study may be a feasible basis for a prospective study comparing our ultrasound-assisted epidural placements to conventional landmark-based techniques.
Bacterial infections in patients with COVID-19: the impact of procalcitonin testing on antibiotics prescription in the real world
Authors: Lui Grace CY , Cheung Catherine SK , Yip Terry CF , Lai Mandy SM , Li Timothy CM , Wong Grace LH
Published: January 2024

Background

Bacterial infections are not prevalent among patients hospitalized with COVID-19, while unnecessary prescription of antibiotics was commonly observed. This study aimed to determine the impact of procalcitonin testing on antibiotics prescription in the real-world setting.

Methods

We performed a territory-wide retrospective cohort study involving all laboratory-confirmed patients hospitalized in public hospitals in Hong Kong in 2020 with COVID-19. We determined the prevalence of bacterial co-infections (documented infections within 72 h of admission) and secondary bacterial infections (infections after 72 h of admission) and antibiotics consumption, and the correlation between procalcitonin testing and antibiotics prescription.

Results

The cohort included 8666 patients, with mean age 45.3 ± 19.9 years, 48.5% male, and comorbidities in 26.9%. Among 2688 patients with bacterial cultures performed, 147 (5.5%) had bacterial co-infections, and 222 (8.3%) had secondary bacterial infections. Antibiotics were prescribed for 2773 (32.0%) patients during the hospital admission. Procalcitonin tests were performed for 2543 (29.3%) patients. More patients with procalcitonin testing received antibiotics (65.9% vs. 17.9%, p < 0.001). Procalcitonin testing was associated with 5-fold increased risk of antibiotics prescription after adjusting for confounding variables. At hospital level, procalcitonin testing correlated with antibiotics prescription. Patients with procalcitonin level < 0.5 ng/mL had a lower probability of antibiotics initiation and shorter duration of antibiotics therapy.

Conclusions

Procalcitonin testing was not associated with lower prescription of antibiotics. Patients with low procalcitonin level had lower antibiotics exposure, supporting the use of procalcitonin to exclude bacterial infections aiding early stopping of antibiotics among patients hospitalized with COVID-19.