bluuebunny/crossref_metadata_2025 huggingface dataset:
Roles of microRNA-192 in diabetic nephropathy: the clinical applications and mechanisms of action
Authors: Wan Xiaoqing , Liao Jian , Lai Hongting , Zhang Shilong , Cui Jianling , Chen Chunyan
Published: June 2023

Diabetic nephropathy (DN) is one of the most common and intractable microvascular complications of diabetes worldwide, serving as the main cause of terminal renal disease. Due to the lack of early specific symptoms and diagnostic markers, DN severely threatens the sufferer’s life. MicroRNA-192 (miR-192) was early identified in human renal cortical tissue and stored and excreted in urine as microvesicles. MiR-192 was found to be involved in the development of DN. For the first time, the present review summarized all the current evidence on the topic of the roles of miR-192 in DN. Finally, 28 studies (ten clinical trials and eighteen experimental studies) were eligible for thorough reviewing. Most of the clinical trials (7/10, 70%) indicated miR-192 might be a protective factor for DN development and progression, while the majority of experimental studies (14/18, 78%) suggested miR-192 might be a pathogenic factor for DN. Mechanistically, miR-192 interacts with various direct targeted proteins (i.e., ZEB1, ZEB2, SIP1, GLP1R, and Egr1) and signaling cascades (i.e., SMAD/TGF-β and PTEN/PI3K/AKT), together contribute to the pathogenesis of DN through epithelial-to-mesenchymal transition (EMT), extracellular matrix deposition, and fibrosis formation. The current review highlights the dual role of miR-192 in the development of DN. Low serum miR-192 expression could be applied for the early prediction of DN (the early stage of DN), while the high miR-192 level in renal tissues and urine may imply the progression of DN (the late stage of DN). Further investigations are still warranted to illustrate this inconsistent phenomenon, which may facilitate promoting the therapeutic applications of miR-192 in predicting and treating DN.
Maternal glucose intolerance during pregnancy affects offspring POMC expression and results in adult metabolic alterations in a sex-dependent manner
Authors: Martins Marina Galleazzo , Silver Zachary , Ayoub Kiara , Hyland Lindsay , Woodside Barbara , Kiss Ana Carolina Inhasz , Abizaid Alfonso
Published: June 2023

Introduction

Gestational diabetes (GDM) is associated with negative outcomes in mothers and their offspring, including greater risks of macrosomia at birth and the development of metabolic disorders. While these outcomes are well-established, the mechanisms by which this increased metabolic vulnerability is conferred on the offspring are comparatively lacking. One proposed mechanism is that maternal glycemic dysregulation alters the development of the hypothalamic regions related to metabolism and energy balance.

Methods

To investigate this possibility, in this study, we first examined the effects of STZ-induced maternal glucose intolerance on the offspring on pregnancy day (PD) 19, and, in a second experiment, in early adulthood (postnatal day (PND) 60). Whether effects would be influenced by sex, or exposure of offspring to a high-fat diet was also investigated. The impact of maternal STZ treatment on POMC neuron number in the ARC of offspring at both time points was also examined.

Results

As expected, STZ administration on PD 7 decreased maternal glucose tolerance, and increased risk for macrosomia, and loss of pups at birth. Offspring of STZ-treated mothers were also more vulnerable to developing metabolic impairments in adulthood. These were accompanied by sex-specific effects of maternal STZ treatment in the offspring, including fewer POMC neurons in the ARC of female but not male infants in late pregnancy and a higher number of POMC neurons in the ARC of both male and female adult offspring of STZ-treated dams, which was exacerbated in females exposed to a high-fat diet after weaning.

Discussion

This work suggests that maternal hyperglycemia induced by STZ treatment, in combination with early-life exposure to an obesogenic diet, leads to adult metabolic alterations that correlate with the increased hypothalamic expression of POMC, showing that maternal glycemic dysregulation can impact the development of hypothalamic circuits regulating energy state with a stronger impact on female offspring.
Higher visceral adiposity index was associated with an elevated prevalence of gallstones and an earlier age at first gallstone surgery in US adults: the results are based on a cross-sectional study
Authors: Zhang Gaopeng , Ding Zhe , Yang Junping , Wang Tianqi , Tong Li , Cheng Jian , Zhang Chao
Published: June 2023

Objective

We sought to evaluate the association between visceral adiposity index (VAI) and the incidence of gallstones and the age at first gallstone surgery in adults in the United States.

Methods

We selected individuals from the National Health and Nutrition Examination Survey (NHANES) database from 2017 to 2020 and evaluated the association between VAI and gallstone incidence and age at first gallstone surgery using logistic regression analysis, subgroup analysis, and dose–response curves.

Results

A total of 7,409 participants aged >20 years were included in our study; 767 had a self-reported history of gallstones. After adjustment for all confounding factors, for each unit of VAI after Ln conversion, gallstone prevalence increased by 31% (OR = 1.31, 95% CI: 1.17, 1.48), while the first gallstone surgery was 1.97 years earlier (β = −1.97, 95% CI: −3.35, −0.42). The dose–response curves showed a positive correlation between VAI and gallstone prevalence. There was a negative correlation between increased VAI and age at first gallstone surgery.

Conclusion

A higher VAI is positively associated with the prevalence of gallstones and may lead to an earlier age at first gallstone surgery. This is worthy of attention, although causality cannot be established.
Metabolic alteration of circulating steroid hormones in women with gestational diabetes mellitus and the related risk factors
Authors: Yang Na , Zhang Wei , Ji Cheng , Ge Jiajia , Zhang Xiaoli , Li Meijuan , Wang Min , Zhang Tianqi , He Jun , Zhu Huaijun
Published: June 2023

Background

Abnormally changed steroid hormones during pregnancy are closely related to the pathological process of gestational diabetes mellitus (GDM). Our aim was to systematically profile the metabolic alteration of circulating steroid hormones in GDM women and screen for risk factors.

Methods

This study was a case-control study with data measured from 40 GDM women and 70 healthy pregnant women during their 24-28 gestational weeks. 36 kinds of steroid hormones, including 3 kinds of corticosteroids, 2 kinds of progestins, 5 kinds of androgens and 26 kinds of downstream estrogens in serum were systematically measured using a combined sensitive UPLC-MS/MS method. The flux of different metabolic pathways of steroid hormones was analyzed. Logistic regression and ROC curve model analyses were performed to identify potential steroid markers closely associated with GDM development.

Results

Serum corticosteroids, progestins and almost all the estrogen metabolites via 16-pathway from parent estrogens were higher in GDM women compared with healthy controls. Most of the estrogen metabolites via 4-pathway and more than half of the metabolites via 2-pathway were not significantly different. 16α-hydroxyestrone (16OHE1), estrone-glucuronide/sulfate (E1-G/S) and the ratio of total 2-pathway estrogens to total estrogens were screened as three indicators closely related to the risk of GDM development. The adjusted odds ratios of GDM for the highest quartile compared with the lowest were 72.22 (95% CI 11.27-462.71, Ptrend<0.001) for 16OHE1 and 6.28 (95% CI 1.74-22.71, Ptrend<0.05) for E1-G/S. The ratio of 2-pathway estrogens to total estrogens was negatively associated with the risk of GDM.

Conclusion

The whole metabolic flux from cholesterol to downstream steroid hormones increased in GDM condition. The most significant changes were observed in the 16-pathway metabolism of estrogens, rather than the 2- or 4-pathway or other types of steroid hormones. 16OHE1 may be a strong marker associated with the risk for GDM.
Performance evaluation of a multi-stage solar distiller associated with Fresnel lens in Malaysian weather
Authors: Yeang Jonathan Yong Kai , Bahar Rubina , Koo Chai Hoon , Lee Sze Shin
Published: June 2023

Water scarcity is widely known as one of the major current issues. As of now, many solar distillers are using the same concept of evaporation and condensation of saline water to produce distillates. The main problem with such solar distillers is their low productivity, as the latent heat produced is lost to the surroundings. A multi-stage solar distiller can solve the issue of productivity by utilizing the latent heat released. This design consists of multiple solar distillers stacked one on top of the another, producing distillates in each stage while having the same amount of energy intake. Malaysia falls within the tropical belt where solar radiation appears to be diffused, resulting in low-quality heat energy that can be absorbed. To solve this problem, concentrated solar power (CSP) technology has been introduced. However, CSP technology can be very expensive, thus using a Fresnel lens as a direct refraction device will be a cheaper option. In this study, a four-stage solar distiller system integrated with the Fresnel lens is introduced. It was found that the productivity and efficiency of the multi-stage system were higher, with a productivity of 0.164 g/kJ and efficiency of 39.5% when compared with the single-stage solar distiller with a productivity of 0.104 g/kJ and an efficiency of 23.5%.
Intelligent grid load forecasting based on BERT network model in low-carbon economy
Authors: Tao Peng , Ma Hao , Li Chong , Liu Linqing
Published: June 2023

In recent years, the reduction of high carbon emissions has become a paramount objective for industries worldwide. In response, enterprises and industries are actively pursuing low-carbon transformations. Within this context, power systems have a pivotal role, as they are the primary drivers of national development. Efficient energy scheduling and utilization have therefore become critical concerns. The convergence of smart grid technology and artificial intelligence has propelled transformer load forecasting to the forefront of enterprise power demand management. Traditional forecasting methods relying on regression analysis and support vector machines are ill-equipped to handle the growing complexity and diversity of load forecasting requirements. This paper presents a BERT-based power load forecasting method that leverages natural language processing and image processing techniques to enhance the accuracy and efficiency of transformer load forecasting in smart grids. The proposed approach involves using BERT for data preprocessing, analysis, and feature extraction on long-term historical load data from power grid transformers. Multiple rounds of training and fine-tuning are then conducted on the BERT architecture using the preprocessed training datasets. Finally, the trained BERT model is used to predict the transformer load, and the predicted results are compared with those obtained based on long short-term memory (LSTM) and actual composite values. The experimental results show that compared with LSTM method, the BERT-based model has higher short-term power load prediction accuracy and feature extraction capability. Moreover, the proposed scheme enables high levels of accuracy, thereby providing valuable support for resource management in power dispatching departments and offering theoretical guidance for carbon reduction initiatives.
Long-term effects of soft rock amendment on changes of soil aggregate cementing agents of sandy soil by SEM-EDS
Authors: Sun Zenghui , Liu Zhe , Han Jichang , Wang Huanyuan , Zhang Haiou , Yan Jiakun
Published: June 2023

Soil aggregates are a crucial constituent of soil and have a significant function in regulating water, nutrients, air, and heat within the soil. The development of soil aggregates is influenced by various factors, including the soil’s parent material and human activities. Understanding the formation and the mechanism of stabilization of soil aggregates is of great significance in the study of soil development, in regulating and managing organic carbon pools in soils, and in promoting soil fertility. In this study, aeolian sandy soil with a low degree of soil development and compound soil formed by combining soft rock and aeolian sandy soil were selected as the research objects. We selected three time points from 0 to 9 years after amendment by soft rock in order to investigate the changes of soil aggregate cementing agents. The shape of soil aggregates in both types of soils was analyzed by environmental scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS), which were also used to assess the appearance of soil aggregates and quantify the composition of mineral elements on a cross section of the aggregate. The results show that when the soft rock and the aeolian sandy soil are compounded and mixed, the clay minerals in the soft rock change the microstructure of the original aeolian sandy soil from a single granular barrier to one characterized by a cumulative porous structure, indicating that clay minerals promote soil development and form aggregates with good structural properties. The cementing agents in the compounded soil aggregates are mainly clay minerals, aluminum, iron, and calcium. In comparison to aeolian sandy soils, the presence of iron and calcium in compounded soils is notably elevated. The iron oxides present in compounded soils serve a similar function to “bolts” in the formation of soil aggregates. These findings establish a theoretical foundation for investigating the process of soil aggregate formation and the mechanisms by which cementing agents contribute to their stabilization.
Unraveling spatiotemporal patterns and multiple driving factors of surface ozone across China and its urban agglomerations management strategies
Authors: Kong Shaojie , Wang Teng , Li Fei , Yan Jingjing , Qu Zhiguang
Published: June 2023

Since State Council launched the Action Plan for Air Pollution Prevention and Control in 2013, national concentration of fine particulate matter (PM2.5) has continued to decline in China, while surface ozone (O3) pollution shows an obvious rise. To identity hot regions and develop targeted policy, the spatiotemporal O3 variation and its population-weighted exposure features were analyzed in 337 cities across China, using autocorrelation analysis and grid exposure calculation. In the identified hot urban agglomerations, the correlation analysis and geographic weighted regression model (GWR) were used to study related meteorological factors and socioeconomic driving factors. O3 pollution and its human exposure were found to have significant spatial aggregation characteristics, showing a need for regional management policy. Beijing-Tianjin-Hebei Urban Agglomeration (BTH-UA), Central Plains Urban Agglomeration (CP-UA), and Yangtze River Delta Urban Agglomeration (YRD-UA) were identified as hot regions where O3 concentration exceeded 160 μg·m−3, exceedance rate was over 20% and population-weighted exposure risk was relatively high. Correlation analysis in the hot regions indicated high surface temperature, low relative humidity, and low wind speed were positive to O3 increase. Further, GWR results revealed that O3 in the majority of cities was positively related with population density (PD), the per capita GDP (Per_GDP), industrial soot emissions (ISE), industrial SO2 emissions (ISO2), and average annual concentration of inhaled fine particulate matter (PM10), and negatively related with total land area of administrative region (Administration) and area of green land (Green). From the regional driving factor difference, the targeted UA management policy was provided.
Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regime
Authors: Sanka Loganathachetti Dinesh , Mundra Sunil
Published: June 2023

Date palms are widely cultivated in arid agroecosystems, where knowledge of irrigation water effect on their soil and root-associated bacterial communities is limited. Using 16S rDNA metabarcoding, we studied soil and root-associated bacterial communities of date palms growing in United Arab Emirates. Overall, 12.18% of bacterial operational taxonomic units (OTUs) were unique to roots, whereas 41.55% were specific to soil. The bacterial diversity was lower in root and community patterns were distinct between compartments, wherein irrigation water pH was a key structuring factor in both compartments, while salinity (electrical conductivity) was important only in the soil. Co-occurrence network analysis revealed a decrease in complexity in the soil–root continuum, and specific taxa/modules also varied with water pH. We observed a higher abundance of endophyte–saprotroph (Bacillus, Streptomyces and Dongia) dual-role OTUs in both compartments, possibly involved in nutrient mobilisation and plant growth. Based on PICRUSt and trait-based analyses, we showed that these OTUs are putatively involved in the nitrogen cycle (nitrification, denitrification, and assimilatory nitrate reduction). Taken together, we suggest that irrigation water pH, not soil pH, transiently affects belowground bacterial communities and selects bacteria in specific pH ranges, which may be important for nutrient cycling (i.e. nitrogen cycle) in arid agroecosystems.
No impact of prenatal paracetamol and folic acid exposure on cord blood DNA methylation in children with attention-deficit/hyperactivity disorder
Authors: Olstad Emilie Willoch , Nordeng Hedvig Marie Egeland , Lyle Robert , Gervin Kristina
Published: June 2023

Pharmacoepigenetic studies are important to understand the mechanisms through which medications influence the developing fetus. For instance, we and others have reported associations between prenatal paracetamol exposure and offspring DNA methylation (DNAm). Additionally, folic acid (FA) intake during pregnancy has been associated with DNAm in genes linked to developmental abnormalities. In this study, we aimed to: (i) expand on our previous findings showing differential DNAm associated with long-term prenatal paracetamol exposure in offspring with attention-deficit/hyperactivity disorder (ADHD), and (ii) examine if there is an interaction effect of FA and paracetamol on DNAm in children with ADHD. We used data from the Norwegian Mother, Father and Child Cohort Study (MoBa) and the Medical Birth Registry of Norway (MBRN). We did not identify any impact of paracetamol or any interaction effect of paracetamol and FA on cord blood DNAm in children with ADHD. Our results contribute to the growing literature on prenatal pharmacoepigenetics, but should be replicated in other cohorts. Replication of pharmacoepigenetic studies is essential to ensure robust findings and to increase the clinical relevance of such studies.
Quantum vaccinomics platforms to advance in vaccinology
Authors: de la Fuente José , Contreras Marinela
Published: June 2023

The opinion flows from Introduction to the immunological quantum that requires a historical perspective, to Quantum vaccine algorithms supported by a bibliometric analysis, to Quantum vaccinomics describing from our perspective the different vaccinomics and quantum vaccinomics algorithms. Finally, in the Discussion and conclusions we propose novel platforms and algorithms developed to further advance on quantum vaccinomics. In the paper we refer to protective epitopes or immunological quantum for the design of candidate vaccine antigens, which may elicit a protective response through both cellular and antibody mediated mechanisms of the host immune system. Vaccines are key interventions for the prevention and control of infectious diseases affecting humans and animals worldwide. Biophysics led to quantum biology and quantum immunology reflecting quantum dynamics within living systems and their evolution. In analogy to quantum of light, immune protective epitopes were proposed as the immunological quantum. Multiple quantum vaccine algorithms were developed based on omics and other technologies. Quantum vaccinomics is the methodological approach with different platforms used for the identification and combination of immunological quantum for vaccine development. Current quantum vaccinomics platforms include in vitro, in music and in silico algorithms and top trends in biotechnology for the identification, characterization and combination of candidate protective epitopes. These platforms have been applied to different infectious diseases and in the future should target prevalent and emerging infectious diseases with novel algorithms.
PANoptosis-based molecular subtyping and HPAN-index predicts therapeutic response and survival in hepatocellular carcinoma
Authors: Song Fei , Wang Cheng-Gui , Mao Jia-Zhen , Wang Tian-Lun , Liang Xiao-Liang , Hu Chen-Wei , Zhang Yu , Han Lu , Chen Zhong
Published: June 2023

Background

Hepatocellular carcinoma (HCC) is a highly prevalent and fatal cancer. The role of PANoptosis, a novel form of programmed cell death, in HCC is yet to be fully understood. This study focuses on identifying and analyzing PANoptosis-associated differentially expressed genes in HCC (HPAN_DEGs), aiming to enhance our understanding of HCC pathogenesis and potential treatment strategies.

Methods

We analyzed HCC differentially expressed genes from TCGA and IGCG databases and mapped them to the PANoptosis gene set, identifying 69 HPAN_DEGs. These genes underwent enrichment analyses, and consensus clustering analysis was used to determine three distinct HCC subgroups based on their expression profiles. The immune characteristics and mutation landscape of these subgroups were evaluated, and drug sensitivity was predicted using the HPAN-index and relevant databases.

Results

The HPAN_DEGs were mainly enriched in pathways associated with the cell cycle, DNA damage, Drug metabolism, Cytokines, and Immune receptors. We identified three HCC subtypes (Cluster_1, SFN+PDK4-; Cluster_2, SFN-PDK4+; Cluster_3, SFN/PDK4 intermediate expression) based on the expression profiles of the 69 HPAN_DEGs. These subtypes exhibited distinct clinical outcomes, immune characteristics, and mutation landscapes. The HPAN-index, generated by machine learning using the expression levels of 69 HPAN_DEGs, was identified as an independent prognostic factor for HCC. Moreover, the high HPAN-index group exhibited a high response to immunotherapy, while the low HPAN-index group showed sensitivity to small molecule targeted drugs. Notably, we observed that the YWHAB gene plays a significant role in Sorafenib resistance.

Conclusion

This study identified 69 HPAN_DEGs crucial to tumor growth, immune infiltration, and drug resistance in HCC. Additionally, we discovered three distinct HCC subtypes and constructed an HPAN-index to predict immunotherapeutic response and drug sensitivity. Our findings underscore the role of YWHAB in Sorafenib resistance, presenting valuable insights for personalized therapeutic strategy development in HCC.
Proteasome-dependent senescent tumor cells mediate immunosuppression through CCL20 secretion and M2 polarization in pancreatic ductal adenocarcinoma
Authors: Wu Mengwei , Han Jiashu , Wu Hao , Liu Ziwen
Published: June 2023

The outcome of pancreatic ductal adenocarcinoma (PDAC) remains poor due to few therapeutic options available and challenges with precision therapy to target each tumour’s specific characteristics. In this study, a biologically meaningful patient stratification-prognostic model with therapeutic suggestion value based on tumor senescence was developed and validated in multiple independent cohorts. Further mechanistic investigation based on single-cell transcriptomic data and in vitro experiments revealed that complement derived from non-senescent tumor cells stimulates M1 differentiation and antigen presentation, while senescent tumor cells secrete CCL20 to favor immunosuppressive M2 polarization. Also, senescent phenotype depends on proteasome function, suggesting that high-risk, high-senescence patients may benefit from proteasome inhibitors, which reverse senescence-mediated resistance to conventional chemotherapy and improve outcome. In conclusion, the current study identified senescence as a tumor-specific, hazardous factor associated with immunosuppression in PDAC. Mechanistically, senescence abrogates complement-induced M1 activation and antigen presentation, and upregulates CCL20 to favor M2 polarization. The senescence-related risk model is prognostic and therapeutic-suggestive. In light of the reliance of senescent cells on proteasomal functions, proteasome inhibitors are promising agents for high-risk patients with senescent PDAC.
Effects of the implementation of T90 extension and 52 mm square mesh codend on the bottom trawl hake fishery of the north western Mediterranean
Authors: Ferragut-Perello Francesca , Vasapollo Claudio , Valls Maria , Farriols Maria Teresa , Massutí Enric , Guijarro Beatriz , Joher Sergi , Bibiloni-Socias Marina , Ordines Francesc
Published: June 2023

The Spanish bottom trawl fleet operating in the Mediterranean currently uses 40 mm square mesh codends. Its selectivity is still too low to overcome the overall problem of high proportions of immature specimens in the catches. Even for some target species such as the European hake (Merluccius merluccius), there are high proportions of individuals smaller than the minimum conservation reference size (MCRS). The aim of the present work is to assess the selectivity of three different configurations of the traditional net (TRA) used in the bottom trawl hake fishery off the northwest Iberian Peninsula, each including the following modifications: i) an extension piece made of 90° turned diamond mesh (T90), ii) a 52 mm square meshed codend (52S), and iii) both modifications combined (EXP). The experimental fishing survey applied two methodologies: alternate hauls to compare the different net configurations; and the covered codend method to calculate the selectivity parameters of 52 mm square meshed codend for the main commercial species. Catch comparisons showed no discard reduction using the T90 extension piece for any of the species analysed, nor any selectivity improvement. Conversely, the 52 mm square meshed codend showed a clear discard reduction for M. merluccius and a generalized improvement of selectivity for most commercial species. This improvement raised the 50% retention length (L50) for M. merluccius to 22.2 cm, well above its MCRS (20 cm), allowing to escape 90% of the undersized individuals. However, the implementation of the 52 mm square meshed codend would involve important economic losses for main target species, like M. merluccius and Mullus barbatus, representing up to 32 and 28% of the incomes, respectively. Considering all analysed species, economic losses using the 52 mm square meshed codend would represent 27% of the incomes obtained using the current 40 mm square meshed codend in force. Despite it, transition analyses showed that the yield per recruit of the main target species would recover after two years, and even increase up to 30% and 17% for M. merluccius and M. barbatus, respectively, after the fourth year of the implementation of the 52 mm square meshed codend.
Exploring the application of Corynebacterium glutamicum single cell protein in the diet of flathead grey mullet (Mugil cephalus): effects on growth performance, digestive enzymes activity and gut microbiota
Authors: Bertini Andrea , Natale Silvia , Gisbert Enric , Andrée Karl B. , Concu Danilo , Dondi Francesco , De Cesare Alessandra , Indio Valentina , Gatta Pier Paolo , Bonaldo Alessio , Parma Luca
Published: June 2023

The capacity of utilising a single cell protein (SCP) ingredient coming from Corynebacterium glutamicum was assessed on adult grey mullet (Mugil cephalus) reared in captive conditions. The experiment was carried out using triplicate groups of grey mullet of 68 g average initial body weight. Three diets, SCP0, SCP10 and SCP20 with increasing inclusion of SCP (0%, 10% and 20%) in substitution of soybean, poultry and fish meal were formulated to contain 30% protein, 10% fat and 18.5 Mj/kg feed of digestible energy. After 113 days, fish fed SCP diets presented significantly lower growth performance and a significant lower activity of the alkaline proteases and aminopeptidases compared to fish fed diet without SCP inclusion. Gut microbiota appeared modulated by SCP inclusion being dominated at the phylum level by Fusobacteria in fish fed SCP0 (51.1%), while in fish fed SCP10 (67.3%) and SCP20 (53.2%) Proteobacteria was dominant. Data evinces a deficiency in the protein utilisation as a cause of the poor growth performance in fish fed the SCP diets. A hypothesis has been proposed that an incomplete SCP cell-wall lysis accounts for this outcome because of the particular organisation of the digestive system of grey mullet (which lack of an acidic stomach digestion) and the failing in the development of a functional gizzard (no access to sand in captive conditions). Even though the outcomes of this research were quite unexpected, they will improve our knowledge on the digestive system of flathead grey mullet and provide some theoretical basis for an improved development of low FM and SBM aquafeed for the species.
Harmful algae diversity from a coastal upwelling system detected by high-throughput sequencing
Authors: Ríos-Castro Raquel , Novoa Beatriz , Hernández-Urcera Jorge , Rodríguez Francisco , Figueras Antonio
Published: June 2023

Introduction

In recent years, environmental DNA (eDNA) amplicon sequencing has been used to unveil plankton diversity in the field. Nevertheless, molecular methods, such as this, are rarely used in harmful algal bloom (HAB) monitoring programs, which mainly rely on morphological identification by conventional light microscopy.

Methods

The present study focused on a shallow marine environment (Ría de Vigo, Northwest Spain), where sediment and plankton samples were collected from 2016 to 2018.

Results

The application of eDNA amplicon sequencing allowed us to simultaneously detect 25 potential harmful species (mainly diatoms and dinoflagellates) included in the IOC-UNESCO Taxonomic Reference List of Harmful Microalgae. Among these, causative agents of amnesic shellfish poisoning (Pseudo-nitzschia spp.) paralytic shellfish poisoning (Gymnodinium catenatum and Alexandrium minutum), azaspiracid producers (Azadinium poporum) and ichthyotoxic haptophytes (Chrysochromulina leadbeateri), were identified. Some toxic microalgae were better represented in sediment (e.g., Pseudo-nitzschia pungens, Gymnodinium catenatum) or planktonic fractions (e.g., Pseudo-nitzschia, Gymnodinium smaydae), confirming the importance of including both sediment and plankton fractions in eDNA monitoring studies. Despite the limitations of sequencing short amplicons, it was possible to discern in this study six Pseudo-nitzschia species and associate each of them with each seasonal peak produced in summer periods. Furthermore, several species previously unreported in Ría de Vigo (Pseudo-nitzschia turgidula, Chrysochromulina leadbeateri, Azadinium poporum) could be detected.

Discusion

These results point out the application of eDNA amplicon sequencing to expand our knowledge about harmful species in HAB monitoring programs and early warning systems for low abundant and rare taxa.
Experimental study on the variation rule of water level outside the pit caused by dewatering in the pit and the rule of reinjection outside the pit
Authors: Yang Qingyuan , Chen Song , Yuan Ying
Published: June 2023

Studying the surface settlement caused by foundation pit dewatering has certain practical significance for engineering construction. When the aquifer in the foundation pit is located within a large burial depth range, the impermeable wall does not completely separate the water layer, resulting in water inside and outside the foundation pit communicating around the bottom of the impermeable wall. Taking the foundation pit project of a subway station in Shenzhen as the background, model experiments were conducted to reveal the changes in water level outside the pit caused by precipitation under different conditions inside the pit. (1) Considering the influence of surrounding seepage, the precipitation curve outside the pit presents a concave and then convex form, which is different from the Dupuit theory precipitation curve. (2) For large foundation pit dewatering in layered soil, the downward trend of the dewatering curve outside the foundation pit decreases with the increase of soil depth. (3) Compared to vertical reinjection in close proximity to dense buildings, inclined reinjection has a smaller impact on the side adjacent to the retaining wall, while it is far away from the retaining wall, with a larger impact range. The full well deep reinjection well with an angle of 20° from the vertical direction has the largest reinjection range and the best reinjection effect. It provides a research basis for reasonable control and prevention of surface settlement and adverse deformation of diaphragm walls in narrow spaces under subsequent surrounding seepage conditions.
Case report: Injected corticosteroids for treating leprosy isolated neuritis
Authors: Spitz Clarissa Neves , Pitta Izabela Jardim Rodrigues , Andrade Ligia Rocha , Sales Anna Maria , Sarno Euzenir Nunes , Villela Nivaldo Ribeiro , Pinheiro Roberta Olmo , Jardim Marcia Rodrigues
Published: June 2023

One of the main manifestations of leprosy is peripheral nerve impairment. Early diagnosis and treatment are important to reduce the impact of neurological impairment, which can cause deformities and physical disabilities. Leprosy neuropathy can be acute or chronic, and neural involvement can occur before, during, or after multidrug therapy, and especially during reactional episodes when neuritis occurs. Neuritis causes loss of function in the nerves and can be irreversible if left untreated. The recommended treatment is corticosteroids, usually through an oral regimen at an immunosuppressive dose. However, patients with clinical conditions that restrict corticosteroid use or that have focal neural involvement may benefit from the use of ultrasound-guided perineural injectable corticosteroids. In this study, we report two cases that demonstrate how the treatment and follow-up of patients with neuritis secondary to leprosy, using new techniques, can be provided in a more individualized way. Nerve conduction studies in association with neuromuscular ultrasound were used to monitor the response to treatment with injected steroids, focusing on neural inflammation. This study provides new perspectives and options for this profile of patients.
Regulation of IFNβ expression: focusing on the role of its promoter and transcription regulators
Authors: Fan Jiqiang , Li Qiumei , Liang Jiadi , Chen Zhirong , Chen Linqin , Lai Junzhong , Chen Qi
Published: June 2023

IFNβ is a single-copy gene without an intron. Under normal circumstances, it shows low or no expression in cells. It is upregulated only when the body needs it or is stimulated. Stimuli bind to the pattern recognition receptors (PRRs) and pass via various signaling pathways to several basic transcriptional regulators, such as IRFs, NF-кB, and AP-1. Subsequently, the transcriptional regulators enter the nucleus and bind to regulatory elements of the IFNβ promoter. After various modifications, the position of the nucleosome is altered and the complex is assembled to activate the IFNβ expression. However, IFNβ regulation involves a complex network. For the study of immunity and diseases, it is important to understand how transcription factors bind to regulatory elements through specific forms, which elements in cells are involved in regulation, what regulation occurs during the assembly of enhancers and transcription complexes, and the possible regulatory mechanisms after transcription. Thus, this review focuses on the various regulatory mechanisms and elements involved in the activation of IFNβ expression. In addition, we discuss the impact of this regulation in biology.
Isoacids supplementation improves growth performance and feed fiber digestibility associated with ruminal bacterial community in yaks
Authors: Jiang Fei , Gao Yanhua , Peng Zhongli , Ma Xiulian , You Yinjie , Hu Zhibin , He Anxiang , Liao Yupeng
Published: June 2023

Introduction

This study was conducted to assess the effect of mixed isoacid (MI) supplementation on fermentation characteristics, nutrient apparent digestibility, growth performance, and rumen bacterial community in yaks.

Methods

A 72-h in vitro fermentation experiment was performed on an ANKOM RF gas production system. MI was added to five treatments at doses of 0, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5% on the dry matter (DM) basis of substrates using a total of 26 bottles (4 bottles per treatment and 2 bottles as the blank). Cumulative gas production was measured at 4, 8, 16, 24, 36, 48, and 72 h. Fermentation characteristics including pH, the concentration of volatile fatty acids (VFAs), ammonia nitrogen (NH3-N), microbial proteins (MCP), and the disappearance rate of dry matter (DMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD) were measured after a 72-h in vitro fermentation to determine an optimal MI dose. Fourteen Maiwa male yaks (180–220 kg, 3–4 years old of age) were randomly assigned to the control group (without MI, n = 7) and the supplemented MI group (n = 7, supplemented with 0.3% MI on DM basis) for the 85-d animal experiment. Growth performance, nutrient apparent digestibility, rumen fermentation parameters, and rumen bacterial diversity were measured.

Results

Supplementation with 0.3% MI achieved the greatest propionate and butyrate content, NDFD and ADFD compared with other groups (P < 0.05). Therefore, 0.3% was used for the animal experiment. Supplementation with 0.3% MI significantly increased the apparent digestibility of NDF and ADF (P < 0.05), and the average daily weight gain of yaks (P < 0.05) without affecting the ruminal concentration of NH3-N, MCP, and VFAs. 0.3% MI induced rumen bacteria to form significantly different communities when compared to the control group (P < 0.05). g__norank_f__Bacteroidales_BS11_gut_group, g__norank_f__Muribaculaceae, g__Veillonellaceae_UCG-001, g__Ruminococcus_gauvreauii_group, g__norank_f__norank_o__RF39 and g__Flexilinea were identified as the biomarker taxa in responding to supplementation with 0.3% MI. Meanwhile, the abundance of g__Flexilinea and g__norank_f__norank_o__RF39 were significantly positively correlated with the NDF digestibility (P < 0.05).

Conclusion

In conclusion, supplementation with 0.3% MI improved the in vitro rumen fermentation characteristics, feed fiber digestibility, and growth performance in yaks, which was associated with changes of the abundance of g__Flexilinea and g__norank_f__norank_o__RF39.
Determining resident microbial community members and their correlations with geochemistry in a serpentinizing spring
Authors: Trutschel Leah R. , Kruger Brittany R. , Sackett Joshua D. , Chadwick Grayson L. , Rowe Annette R.
Published: June 2023

Terrestrial serpentinizing systems allow us insight into the realm of alkaliphilic microbial communities driven by geology in a way that is frequently more accessible than their deep subsurface or marine counterparts. However, these systems are also marked by geochemical and microbial community variation due to the interactions of serpentinized fluids with host geology and the surface environment. To separate the transient from the endemic microbes in a hyperalkaline environment, we assessed the Ney Springs terrestrial serpentinizing system microbial community and geochemistry at six time points over the span of a year. Using 16S rRNA gene surveys we observed 93 amplicon sequence variants (ASVs) that were found at every sampling event. This is compared to ~17,000 transient ASVs that were detected only once across the six sampling events. Of the resident community members, 16 of these ASVs were regularly greater than 1% of the community during every sampling period. Additionally, many of these core taxa experienced statistically significant changes in relative abundance with time. Variation in the abundance of some core populations correlated with geochemical variation. For example, members of the Tindallia group, showed a positive correlation with variation in levels of ammonia at the spring. Investigating the metagenome assembled genomes of these microbes revealed evidence of the potential for ammonia generation via Stickland reactions within Tindallia. This observation offers new insight into the origin of high ammonia concentrations (>70 mg/L) seen at this site. Similarly, the abundance of putative sulfur-oxidizing microbes like Thiomicrospira, Halomonas, and a Rhodobacteraceae species could be linked to changes observed in sulfur-oxidation intermediates like tetrathionate and thiosulfate. While these data supports the influence of core microbial community members on a hyperalkaline spring’s geochemistry, there is also evidence that subsurface processes affect geochemistry and may impact community dynamics as well. Though the physiology and ecology of these astrobiologically relevant ecosystems are still being uncovered, this work helps identify a stable microbial community that impacts spring geochemistry in ways not previously observed in serpentinizing ecosystems.
Bacteriophage-mediated biosynthesis of MnO2NPs and MgONPs and their role in the protection of plants from bacterial pathogens
Authors: Ogunyemi Solabomi Olaitan , Abdallah Yasmine , Ibrahim Ezzeldin , Zhang Yang , Bi Ji’an , Wang Fang , Ahmed Temoor , Alkhalifah Dalal Hussien M. , Hozzein Wael N. , Yan Chengqi , Li Bin , Xu Lihui
Published: June 2023

Introduction

Xanthomonas oryzae pv. oryzae (Xoo) is the plant pathogen of Bacterial Leaf Blight (BLB), which causes yield loss in rice.

Methods

In this study, the lysate of Xoo bacteriophage X3 was used to mediate the bio-synthesis of MgO and MnO2. The physiochemical features of MgONPs and MnO2NPs were observed via Ultraviolet - Visible spectroscopy (UV–Vis), X-ray diffraction (XRD), Transmission/Scanning electron microscopy (TEM/SEM), Energy dispersive spectrum (EDS), and Fourier-transform infrared spectrum (FTIR). The impact of nanoparticles on plant growth and bacterial leaf blight disease were evaluated. Chlorophyll fluorescence was used to determine whether the nanoparticles application were toxic to the plants.

Results

An absorption peak of 215 and 230 nm for MgO and MnO2, respectively, confirmed nanoparticle formation via UV–Vis. The crystalline nature of the nanoparticles was detected by the analysis of XRD. Bacteriological tests indicated that MgONPs and MnO2NPs sized 12.5 and 9.8 nm, respectively, had strong in vitro antibacterial effects on rice bacterial blight pathogen, Xoo. MnO2NPs were found to have the most significant antagonist effect on nutrient agar plates, while MgONPs had the most significant impact on bacterial growth in nutrient broth and on cellular efflux. Furthermore, no toxicity to plants was observed for MgONPs and MnO2NPs, indeed, MgONPs at 200 μg/mL significantly increased the quantum efficiency of PSII photochemistry on the model plant, Arabidopsis, in light (ΦPSII) compared to other interactions. Additionally, significant suppression of BLB was noted in rice seedlings amended with the synthesized MgONPs and MnO2NPs. MnO2NPs showed promotion of plant growth in the presence of Xoo compared to MgONPs.

Conclusion

An effective alternative for the biological production of MgONPs and MnO2NPs was reported, which serves as an effective substitute to control plant bacterial disease with no phytotoxic effect.
HSV-1 selectively packs the transcription factor Oct-1 into EVs to facilitate its infection
Authors: Ma Yilei , Deng Xiaomei , Zhou Lingyue , Dong Hongchang , Xu Pei
Published: June 2023

HSV-1 hijacks the cellular vesicular secretion system and promotes the secretion of extracellular vesicles (EVs) from infected cells. This is believed to facilitate the maturation, secretion, intracellular transportation and immune evasion of the virus. Intriguingly, previous studies have shown that noninfectious EVs from HSV-1-infected cells exert antiviral effects on HSV-1 and have identified host restrictive factors, such as STING, CD63, and Sp100 packed in these lipid bilayer-enclosed vesicles. Octamer-binding transcription factor-1 (Oct-1) is shown here to be a pro-viral cargo in non-virion-containing EVs during HSV-1 infection and serves to facilitate virus dissemination. Specifically, during HSV-1 infection, the nuclear localized transcription factor Oct-1 displayed punctate cytosolic staining that frequently colocalized with VP16 and was increasingly secreted into the extracellular space. HSV-1 grown in cells bereft of Oct-1 (Oct-1 KO) was significantly less efficient at transcribing viral genes during the next round of infection. In fact, HSV-1 promoted increased exportation of Oct-1 in non-virion-containing EVs, but not the other VP16-induced complex (VIC) component HCF-1, and EV-associated Oct-1 was promptly imported into the nucleus of recipient cells to facilitate the next round of HSV-1 infection. Interestingly, we also found that EVs from HSV-1-infected cells primed cells for infection by another RNA virus, vesicular stomatitis virus. In summary, this investigation reports one of the first pro-viral host proteins packed into EVs during HSV-1 infection and underlines the heterogenetic nature and complexity of these noninfectious double-lipid particles.
Running from stress: a perspective on the potential benefits of exercise-induced small extracellular vesicles for individuals with major depressive disorder
Authors: Khoury Reine , Nagy Corina
Published: June 2023

Aerobic exercise promotes beneficial effects in the brain including increased synaptic plasticity and neurogenesis and regulates neuroinflammation and stress response via the hypothalamic-pituitary-adrenal axis. Exercise can have therapeutic effects for numerous brain-related pathologies, including major depressive disorder (MDD). Beneficial effects of aerobic exercise are thought to be mediated through the release of “exerkines” including metabolites, proteins, nucleic acids, and hormones that communicate between the brain and periphery. While the specific mechanisms underlying the positive effects of aerobic exercise on MDD have not been fully elucidated, the evidence suggests that exercise may exert a direct or indirect influence on the brain via small extracellular vesicles which have been shown to transport signaling molecules including “exerkines” between cells and across the blood-brain barrier (BBB). sEVs are released by most cell types, found in numerous biofluids, and capable of crossing the BBB. sEVs have been associated with numerous brain-related functions including neuronal stress response, cell-cell communication, as well as those affected by exercise like synaptic plasticity and neurogenesis. In addition to known exerkines, they are loaded with other modulatory cargo such as microRNA (miRNA), an epigenetic regulator that regulates gene expression levels. How exercise-induced sEVs mediate exercise dependent improvements in MDD is unknown. Here, we perform a thorough survey of the current literature to elucidate the potential role of sEVs in the context of neurobiological changes seen with exercise and depression by summarizing studies on exercise and MDD, exercise and sEVs, and finally, sEVs as they relate to MDD. Moreover, we describe the links between peripheral sEV levels and their potential for infiltration into the brain. While literature suggests that aerobic exercise is protective against the development of mood disorders, there remains a scarcity of data on the therapeutic effects of exercise. Recent studies have shown that aerobic exercise does not appear to influence sEV size, but rather influence their concentration and cargo. These molecules have been independently implicated in numerous neuropsychiatric disorders. Taken together, these studies suggest that concentration of sEVs are increased post exercise, and they may contain specifically packaged protective cargo representing a novel therapeutic for MDD.