bluuebunny/crossref_metadata_2025 huggingface dataset:
A Stiff Extracellular Matrix Favors the Mechanical Cell Competition that Leads to Extrusion of Bacterially-Infected Epithelial Cells
Authors: Aparicio-Yuste Raúl , Muenkel Marie , Clark Andrew G. , Gómez-Benito María J. , Bastounis Effie E.
Published: June 2022

Cell competition refers to the mechanism whereby less fit cells (“losers”) are sensed and eliminated by more fit neighboring cells (“winners”) and arises during many processes including intracellular bacterial infection. Extracellular matrix (ECM) stiffness can regulate important cellular functions, such as motility, by modulating the physical forces that cells transduce and could thus modulate the output of cellular competitions. Herein, we employ a computational model to investigate the previously overlooked role of ECM stiffness in modulating the forceful extrusion of infected “loser” cells by uninfected “winner” cells. We find that increasing ECM stiffness promotes the collective squeezing and subsequent extrusion of infected cells due to differential cell displacements and cellular force generation. Moreover, we discover that an increase in the ratio of uninfected to infected cell stiffness as well as a smaller infection focus size, independently promote squeezing of infected cells, and this phenomenon is more prominent on stiffer compared to softer matrices. Our experimental findings validate the computational predictions by demonstrating increased collective cell extrusion on stiff matrices and glass as opposed to softer matrices, which is associated with decreased bacterial spread in the basal cell monolayer in vitro. Collectively, our results suggest that ECM stiffness plays a major role in modulating the competition between infected and uninfected cells, with stiffer matrices promoting this battle through differential modulation of cell mechanics between the two cell populations.
Data Usability: The Forgotten Segment of Environmental Data Workflows
Authors: Dosemagen Shannon , Williams Emelia
Published: June 2022

While there has been a rapid increase in the use of participatory science methods over the last decade, the usability of resulting data in addressing situations of environmental injustice is often overlooked, neglected, or used as political fuel for ignoring inconvenient truths. The inability of data to be used for policy, regulation, and enforcement impedes its usefulness in various situations depending on user requirements and governance scales. On the other hand, there are vast open datasets that could be useful for communities and researchers, but these data are often difficult to find, use, or repurpose, beyond their original intent. This article unpacks the data usability problem at the frontier of environmental governance and decision-making, suggesting that by prioritizing environmental data as a public good, there are clear mechanisms for ensuring data usability toward participatory environmental governance. The authors are interested in uncovering the policies and behavioral and bureaucratic patterns that have remained static as participatory science methods and tools have advanced. It is necessary to understand where and when associated tools, methods, and platforms have failed to ensure that data is usable and useful for communities attempting deeper engagement and representation in environmental governance.
Perioperative Pain Management With Bilateral Pecto-intercostal Fascial Block in Pediatric Patients Undergoing Open Cardiac Surgery
Authors: Zhang Yang , Min Jia , Chen Shibiao
Published: June 2022

Purposes

Pediatric open cardiac surgical patients usually suffer from acute pain after operation. The current work aimed to explore the impact of bilateral PIFB in children suffering from open cardiac surgery.

Methods

This work randomized altogether 110 child patients as bilateral PIFB (PIF) and non-nerve block (SAL) groups. This work adopted post-operative pain at exercise and rest statuses as the primary endpoint, whereas time-to-drain removal/extubation/initial defecation, intraoperative/post-operative fentanyl use, and length of ICU and hospital stay as the secondary endpoints.

Results

MOPS were significantly higher at 24-h post-operatively at coughing and rest statuses in SAL group compared with PIF group. Meanwhile, PIF group exhibited markedly lower intraoperative/post-operative fentanyl use amounts, as well as markedly reduced time-to-extubation/initial flatus, and length of ICU/hospital stay.

Conclusion

Bilateral PIFB in pediatric open cardiac surgical patients provide effective analgesia and lower the length of hospital stay.
The U-Shaped Relationship Between Serum Uric Acid and Long-Term All-Cause Mortality in Coronary Artery Disease Patients: A Cohort Study of 33,034 Patients
Authors: Zheng Yiying , Ou Jiaman , Huang Dehua , Zhou Ziyou , Dong Xiaoli , Chen Jie , Liang Dandan , Liu Jin , Liu Yong , Chen Jiyan , Huang Xiaoyu , Tan Ning
Published: June 2022

Background

Associations between high serum uric acid (SUA) and cardiovascular diseases have been reported. However, few studies have been conducted to explore the relationship between SUA and long-term all-cause mortality in coronary artery disease (CAD) patients. Our study aims to investigate the relationship between SUA and long-term all-cause mortality in patients with CAD.

Methods

From January 2007 to December 2018, we divided 33,034 patients with CAD admitted in the Guangdong Provincial People's Hospital into five groups (quintile 1: SUA <5.05 mg/dl, quintile 2: 5.05 mg/dl ≤ SUA <5.59 mg/dl, quintile 3:5.59 mg/dl ≤ SUA <6.8 mg/dl, quintile 4, 6.8 mg/dl ≤ SUA <7.93 mg/dl, and quintile 5, SUA ≥7.93 mg/d;). This study used Kaplan–Meier survival analysis to evaluate patient outcomes with different ranges of SUA. Cox proportional hazards regression models and restricted cubic spline were applied to determine the association between serum uric and long-term all-cause mortality.

Results

A total of 33,034 participants were recruited, including 24,780 (75.01%) men and 8,254 (24.99) women in this cohort study. Median follow-up was 4.91 years. We found that SUA is an independent risk factor of long-term all-cause mortality according to the result of Cox proportional hazards models. This study also illustrated an approximate U-shape association between SUA and all-cause mortality when compared with 5.95 mg/lL ≤ SUA <6.8 mg/dl, SUA <5.0 5mg/dl (adjusted hazard ratio (aHR) =1.13, 95% CI: 1.01–1.26, p = 0.03), and SUA ≥8 mg/dL (aHR = 1.18, 95% CI: 1.06-1.32, p = 0.003).

Conclusion

Our study indicated a U-shaped relationship between SUA and long-term all-cause mortality in patients with CAD. No matter whether SUA is too high or too low, it increased the all-cause mortality in the CAD population, which deserves to be closely monitored.
A Comparison of the Geochemical Characteristics of Ultra-Deep Natural Gas in the Kuqa Foreland and Marine Craton Areas in the Tarim Basin, China
Authors: Yu Cong , Ni Yunyan , Dai Jinxing , He Yuanyuan , Fang Chenchen , Liao Fengrong
Published: June 2022

This research analyzed the composition and hydrocarbon isotope geochemical characteristics of ultra-deep natural gas (buried deeper than 6000 m) in more than 130 wells in the Tarim Basin. Characteristics of the ultra-deep natural gas components in the Tarim Basin are as follows: natural gas in the Kuqa sandstone reservoir is developed as dry gas, with a high methane content (an average of 95.53%) and a low ethane content (an average of only 1.39%). The ultra-deep gas of the marine craton carbonate reservoirs in the North Tarim-Central Tarim-East Tarim area is developed as wet gas. It is mainly composed of methane ranging from 56.1% to 98.8%, with an average content of 76.36% and also contains a small amount of ethane amounting to 6.74%. CO2 and H2S contents of ultra-deep condensate gas in the Central Tarim area are high, with maximum values of 24.2% and 23.1%, respectively. Isotopic characteristics are as follows: δ13C1 values of Kuqa natural gas are distributed between −36‰ and −25.3‰, with an average of −28.2‰, δ13C2 values are distributed between −26.2‰ and −13.8‰, with an average of −18.3‰. Most gas samples in Kuqa area are developed with the reversal of carbon isotopic series as δ13C2 > δ13C3, and the δ13CCO2 values are distributed between −19.5‰ and −10.3‰. The δ13C1 values of ultra-deep gas in marine craton carbonate rocks are distributed between −54.4‰ and −33.3‰, with an average of −42.6‰. δ13C2 values are distributed between −41.1‰ and −29.4‰, with an average of −33.8‰, and δ13CCO2 values are distributed between −28.2‰–0.6‰. According to the identification chart, it can be seen that the ultra-deep gas in the sandstone reservoir of the Kuqa foreland is mainly coal-derived gas in the high over-mature stage, and the reversal of carbon isotopic series may be caused by the mixing of natural gas of the same type and different sources. The natural gas in carbonate reservoirs of marine craton is mainly oil-type gas with complex sources, including both kerogen cracking gas and oil cracking gas. CO2 can be formed by organic and inorganic genesis. Inorganic CO2 is most likely to be generated by the dissolution of carbonate rocks under acid formation water.
Long-Term Forecasting of Strong Earthquakes in North America, South America, Japan, Southern China and Northern India With Machine Learning
Authors: Velasco Herrera Victor Manuel , Rossello Eduardo Antonio , Orgeira Maria Julia , Arioni Lucas , Soon Willie , Velasco Graciela , Rosique-de la Cruz Laura , Zúñiga Emmanuel , Vera Carlos
Published: June 2022

Strong earthquakes (magnitude ≥7) occur worldwide affecting different cities and countries while causing great human, ecological and economic losses. The ability to forecast strong earthquakes on the long-term basis is essential to minimize the risks and vulnerabilities of people living in highly active seismic areas. We have studied seismic activities in North America, South America, Japan, Southern China and Northern India in search for patterns in strong earthquakes on each of these active seismic zones between 1900 and 2021 with the powerful mathematical tool of wavelet transform. We found that the primary seismic activity patterns for M ≥ 7 earthquakes are 55, 3.7, 7.7, and 8.6 years, for seismic zones of the southwestern United States and northern Mexico, southwestern Mexico, South American, and Southern China-Northern India, respectively. In the case of Japan, the most important seismic pattern for earthquakes with magnitude 7 ≤ M< 8 is 4.1 years and for strong earthquakes with M ≥ 8, it is 40 years. Every seismic pattern obtained clusters the earthquakes in historical intervals/episodes with and without strong earthquakes in the individually analyzed seismic zones. We want to clarify that the intervals where no strong earthquakes do not imply the total absence of seismic activity because earthquakes can occur with lesser magnitude within this same interval. From the information and pattern we obtained from the wavelet analyses, we created a probabilistic, long-term earthquake prediction model for each seismic zone using the Bayesian Machine Learning method. We propose that the periods of occurrence of earthquakes in each seismic zone analyzed could be interpreted as the period in which the stress builds up on different planes of a fault, until this energy releases through the rupture along faults and fractures near the plate tectonic boundaries. Then a series of earthquakes can occur along the fault until the stress subsides and a new cycle begins. Our machine learning models predict a new period of strong earthquakes between 2040 ± 5 and 2057 ± 5, 2024 ± 1 and 2026 ± 1, 2026 ± 2 and 2031 ± 2, 2024 ± 2 and 2029 ± 2, and 2022 ± 1 and 2028 ± 2 for the five active seismic zones of United States, Mexico, South America, Japan, and Southern China and Northern India, respectively. In additon, our methodology can be applied in areas where moderate earthquakes occur, as for the case of the Parkfield section of the San Andreas fault (California, United States). Our methodology explains why a moderate earthquake could never occur in 1988 ± 5 as proposed and why the long-awaited Parkfield earthquake event occurred in 2004. Furthermore, our model predicts that possible seismic events may occur between 2019 and 2031, with a high probability of earthquake events at Parkfield around 2025 ± 2 years.
Earth’s Orbital Control on Organic Matter Enrichment in the Black Shales of the Wufeng–Longmaxi Formation in the Upper Yangtze Region, South China
Authors: Zhao Shaoze , Li Yong , Xu Yingjiao , Cheng Leli , Nie Zhou , Zhao Liang
Published: June 2022

China’s most successful horizon for shale-gas exploration and development is the Upper Ordovician Wufeng Formation through the Lower Silurian Longmaxi Formation in its Upper Yangtze Region. In this study, the Wufeng–Longmaxi Formation black shales in the Upper Yangtze Region are analysed to determine their microstructural characteristics, total organic carbon (TOC) content, and well log characteristics and to identify information on the parameters of Earth’s orbit from its gamma-ray (GR) data series. Then, paleoenvironmental modes are established over a long time scale. On this basis, the Earth’s orbital control on organic matter enrichment in the black shales is examined. The black shales of the Wufeng Formation and the lower Longmaxi Formation are rich in biogenic siliceous fossils and framboidal pyrite. Their TOC content, GR value, and uranium (U) content increase gradually from the bottom of the Wufeng Formation to the Guanyinqiao Member at its top, peak at the Guanyinqiao Member, and then decrease gradually up to the Longmaxi Formation. Approximately six and seven long eccentricity cycles can be identified from the GR curves of the Wufeng Formation and the lower Longmaxi Formation, respectively. During the long eccentricity minima, corresponding to Earth’s cold period, the black shales have a relatively high level of enrichment of organic matter. This study can provide an important reference for investigating the mechanism by which Earth’s orbits control the climate and sedimentary environment, as well as the mechanism of organic matter enrichment.
Is the Development of Gestational Diabetes Associated With the ABO Blood Group/Rhesus Phenotype?
Authors: Lemaitre M. , Passet M. , Ghesquière L. , Martin C. , Drumez E. , Subtil D. , Vambergue A.
Published: June 2022

Aims

There are few published data on the putative association between the ABO blood group/rhesus (Rh) factor and the risk of developing gestational diabetes mellitus (GDM). Our aim was to explore the link between each one factor and GDM development.

Methods

All women having given birth at Lille University Medical Center (Lille, France) between August 1st, 2017, and February 28th, 2018, were tested for GDM, using the method recommended in the French national guidelines. The risk of GDM was assessed for each ABO blood group, each Rh phenotype and combinations thereof, using logistic regression models.

Results

1194 women had at least one GDM risk factor. The percentage of GDM varied with the ABO group (p=0.013). Relative to group O women, group AB women were more likely to develop GDM (OR = 2.50, 95% CI [1.43 to 4.36], p=0.001). Compared with the Rh-positive O group, only the Rh-positive AB group had an elevated risk of developing GDM (OR = 3.02, 95% CI [1.69 to 5.39], p < 0.001).

Conclusions

Our results showed that Rh-positive group AB women have a greater risk of GDM. With a view to preventing GDM, at-risk individuals could be identified by considering the ABO blood group phenotype either as a single risk factor or in combination with other risk factors.
Effect of Sustainability Claim on Willingness to Pay for Upcycled Food in Digital Era: Differential Effect of Sustainability Claim Between Virtue and Vice Product Category
Authors: Ghazanfar Sidra , Abdullah Muhammad , Ummar Rakhshan , Shabbir Rizwan , Saqib Shahnawaz
Published: June 2022

The growing demand for food availability is persuading researchers, marketers, and consumers to opt for various solutions that could ensure food sustainability for our upcoming generations. Upcycled food is one of the solutions which could lead to food sustainability by upcycling discarded food ingredients. However, marketers seem less interested in producing upcycled food because of their low acceptance and willingness to pay. This study investigated when sustainability claims prompt premium prices for upcycled food; whether it varies between virtue and vice product categories? The research incorporates two experiments on the willingness to pay for upcycled food and examines the role of the intervention product category (virtue vs. vice). Overall, the results confirm the original findings; however, some differences in the context of virtue vs. vice product categories have been observed. The results (study I, without sustainability claim) indicated that consumers showed more willingness to pay for conventional (virtue and vice) products as compared to upcycled (virtue and vice) products. The results further revealed that willingness to pay for vice products was low compared to virtue products, particularly for upcycled vice products. The findings of study II indicated that sustainability claim increases the willingness to pay for upcycled food, both for virtue and vice product categories. However, willingness to pay for upcycled vice products increases more than for upcycled virtue products.
The EIF2AK4/rs4594236 AG/GG Genotype Is a Hazard Factor of Immunoglobulin Therapy Resistance in Southern Chinese Kawasaki Disease Patients
Authors: Yu Hongyan , Liu Fucheng , Chen Kaining , Xu Yufen , Wang Yishuai , Fu Lanyan , Zhou Huazhong , Pi Lei , Che Di , Li Hehong , Gu Xiaoqiong
Published: June 2022

Background: Kawasaki disease (KD) is an acute, self-limited vasculitis disorder of unknown etiology in children. Immunologic abnormalities were detected during the acute phase of KD, which reflected that the effect cells of the activated immune system markedly increased cytokine production. High-dose intravenous immunoglobulin (IVIG) therapy is effective in resolving inflammation from KD and reducing occurrence of coronary artery abnormalities. However, 10%–20% of KD patients have no response to IVIG therapy, who were defined as IVIG resistance. Furthermore, these patients have persistent inflammation and increased risk of developing coronary artery aneurysm (CAA). EIF2AK4 is a stress sensor gene and can be activated by pathogen infection. In addition, the polymorphisms of EIF2AK4 were associated with various blood vessel disorders. However, it remains unclear whether the EIF2AK4 gene polymorphisms were related to IVIG therapy outcome in KD patients.

Methods:EIF2AK4/rs4594236 polymorphism was genotyped in 795 IVIG response KD patients and 234 IVIG resistant KD patients through TaqMan, a real-time polymerase chain reaction. The odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to assess the strength of association between EIF2AK4/rs4594236 polymorphism and IVIG therapeutic effects.

Results: Our results showed that the EIF2AK4/rs4594236 AG/GG genotype was significantly associated with increased risk to IVIG resistance compared to the AA genotype (AG vs. AA: adjusted ORs = 1.71, 95% CIs = 1.17–2.51, and p = 0.0061; GG vs. AA: adjusted ORs = 2.09, 95% CIs = 1.36–3.23, and p = 0.0009; AG/GG vs. AA: adjusted ORs = 1.82, 95% CIs = 1.27–2.63, and p = 0.0013; and GG vs. AA/AG: adjusted ORs = 1.45, 95% CI = 1.04–2.02, and p = 0.0306). Furthermore, the stratified analysis of age and gender in the KD cohort indicated that male patients carrying the rs4594236 AG/GG genotype tends to be more resistant to IVIG therapy than female patients.

Conclusion: These results suggested that EIF2AK4/rs4594236 polymorphism might be associated with increased risk of IVIG resistance in southern Chinese KD patients.
Case Report: Prenatal Diagnosis of Postaxial Polydactyly With Bi-Allelic Variants in Smoothened (SMO)
Authors: Fan Lihong , Jin Pengzhen , Qian Yeqing , Shen Guosong , Shen Xueping , Dong Minyue
Published: June 2022

Postaxial polydactyly is a common congenital malformation which involves complex genetic factors. This retrospective study analyzed the cytogenetic and molecular results of a Chinese fetus diagnosed with postaxial polydactyly of all four limbs. Fetal karyotyping and chromosomal microarray analysis (CMA) did not find any abnormality while trio whole-exome sequencing (trio-WES) identified bi-allelic variants in smoothened (SMO) and (NM_005631.5: c.1219C > G, NP_005622.1: p. Pro407Ala, and NM_005631.5: c.1619C > T, NP_005622.1: p. Ala540Val). Sanger sequencing validated these variants. The mutations are highly conserved across multiple species. In-depth bioinformatics analysis and familial co-segregation implied the compound heterozygous variants as the likely cause of postaxial polydactyly in this fetus. Our findings provided the basis for genetic counseling and will contribute to a better understanding of the complex genetic mechanism that underlies postaxial polydactyly.
Novel RSPH4A Variants Associated With Primary Ciliary Dyskinesia–Related Infertility in Three Chinese Families
Authors: Wang Lin , Wang Rongchun , Yang Danhui , Lu Chenyang , Xu Yingjie , Liu Ying , Guo Ting , Lei Cheng , Luo Hong
Published: June 2022

Background: The radial spoke head component 4A (RSPH4A) is involved in the assembly of radial spokes, which is essential for motile cilia function. Asthenoteratozoospermia in primary ciliary dyskinesia (PCD) related to RSPH4A variants has not been reported.

Materials and Methods:RSPH4A variants were identified and validated using whole-exome and Sanger sequencing in three unrelated Chinese families. High-speed video microscopy analysis (HSVA) was performed to measure the beating frequency and pattern of nasal cilia of the patients and healthy control. Papanicolaou staining and computer-aided sperm analysis were performed to analyze the morphology and motility of the sperm in patient 1. Immunofluorescence was adopted to confirm the structure deficiency of sperm and nasal cilia.

Results: Patient 1 from family 1 is a 22-year-old unmarried male presented with bronchiectasis. Semen analysis and sperm Papanicolaou staining confirmed asthenoteratozoospermia. Novel compound heterozygous RSPH4A variants c.2T>C, p.(Met1Thr) and c.1774_1775del, p.(Leu592Aspfs*5) were detected in this patient. Patients 2 and 3 are from two unrelated consanguineous families; they are both females and exhibited bronchiectasis and infertility. Two homozygous RSPH4A variants c.2T>C, p.(Met1Thr) and c.351dupT, p.(Pro118Serfs*2) were detected, respectively. HSVA showed that most of the cilia in patients 1 and 3 were with abnormal rotational movement. The absence of RSPH4A and RSPH1 in patient 1’s sperm and patient 3’s respiratory cilia was indicated by immunofluorescence. Patient 2 died of pulmonary infection and respiratory failure at the age of 35 during follow-up.

Conclusion: Dysfunctional sperm flagellum and motile cilia in the respiratory tract and the fallopian tube were found in patients with RSPH4A variants. Our study enriches the genetic spectrum and clinical phenotypes of RSPH4A variants in PCD, and c.2T>C, p.(Met1Thr) detected in our patients may be a hotspot RSPH4A variant in Chinese.
Effects of the COVID-19 Pandemic and Telehealth on Antenatal Screening and Services, Including for Mental Health and Domestic Violence: An Australian Mixed-Methods Study
Authors: Henry Amanda , Yang Jennifer , Grattan Sarah , Roberts Lynne , Lainchbury Anne , Shanthosh Janani , Cullen Patricia , Everitt Louise
Published: June 2022

Introduction

Australian antenatal care includes specific screening and service provision for domestic and family violence (DFV) and mental health. However, the COVID-19 pandemic resulted in major care changes, including greatly expanded telehealth. Given difficulties in a safe assessment and management of disclosures via telehealth, DFV and mental health service provision might be substantially impacted. This study therefore aimed to assess COVID-19 effects on DFV and mental health screening, as well as broader service provision from the perspective of local maternity service providers.

Methods

Mixed-methods study of staff surveys and interviews of staff directly involved in pregnancy care (doctors, midwives, and allied health) in three Sydney (Australia) maternity units, from October 2020 to March 2021. Surveys and interviews interrogated perceived effects of the COVID-19 pandemic on delivery (ensuring required services occurred), timeliness, and quality of (a) overall maternity care and (b) DFV and mental health screening and care; and also advantages and disadvantages of telehealth. Surveys were descriptively analyzed. Interviews were conducted online, recorded, and transcribed verbatim prior to thematic analysis.

Results

In total, 17 interviews were conducted and 109 survey responses were received. Breakdown of survey respondents was 67% of midwives, 21% of doctors, and 10% of allied health. Over half of survey respondents felt the pandemic had a negative effect on delivery, timeliness, and quality of overall pregnancy care, and DFV and mental health screening and management. Perceived telehealth positives included convenience for women (73%) and reducing women's travel times (69%). Negative features included no physical examination (90%), difficulty regarding non-verbal cues (84%), difficulty if interpreter required (71%), and unsure if safe to ask some questions (62%). About 50% felt telehealth should continue post-pandemic, but for <25% of visits. Those perceived suitable for telehealth were low-risk and multiparous women, whereas those unsuited were high-risk pregnancy, non-English speaking, and/or mental health/psychosocial/DFV concerns. “Change to delivery of care” was the central interview theme, with subthemes of impact on mental health/DFV screening, telehealth (both positive and negative), staff impact (e.g., continuity of care disruption), and perceived impact on women and partners.

Discussion

While telehealth may have an ongoing, post-pandemic role in Australian maternity care, staff believe that this should be limited in scope, mostly for low-risk pregnancies. Women with high risk due to physical health or mental health, DFV, and/or other social concerns were considered unsuited to telehealth.
Extracellular Vesicles Derived From Ex Vivo Expanded Regulatory T Cells Modulate In Vitro and In Vivo Inflammation
Authors: Thome Aaron D. , Thonhoff Jason R. , Zhao Weihua , Faridar Alireza , Wang Jinghong , Beers David R. , Appel Stanley H.
Published: June 2022

Extracellular vehicles (EVs) are efficient biomarkers of disease and participate in disease pathogenesis; however, their use as clinical therapies to modify disease outcomes remains to be determined. Cell-based immune therapies, including regulatory T cells (Tregs), are currently being clinically evaluated for their usefulness in suppressing pro-inflammatory processes. The present study demonstrates that ex vivo expanded Tregs generate a large pool of EVs that express Treg-associated markers and suppress pro-inflammatory responses in vitro and in vivo. Intravenous injection of Treg EVs into an LPS-induced mouse model of inflammation reduced peripheral pro-inflammatory transcripts and increased anti-inflammatory transcripts in myeloid cells as well as Tregs. Intranasal administration of enriched Treg EVs in this model also reduced pro-inflammatory transcripts and the associated neuroinflammatory responses. In a mouse model of amyotrophic lateral sclerosis, intranasal administration of enriched Treg EVs slowed disease progression, increased survival, and modulated inflammation within the diseased spinal cord. These findings support the therapeutic potential of expanded Treg EVs to suppress pro-inflammatory responses in human disease.
Persistent Antiphospholipid Antibodies Are Not Associated With Worse Clinical Outcomes in a Prospective Cohort of Hospitalised Patients With SARS-CoV-2 Infection
Authors: Espinosa Gerard , Zamora-Martínez Carles , Pérez-Isidro Albert , Neto Daniela , Bravo-Gallego Luz Yadira , Prieto-González Sergio , Viñas Odette , Moreno-Castaño Ana Belen , Ruiz-Ortiz Estíbaliz , Cervera Ricard ,   The COVAPS-CLINIC Study Group Investigators
Published: June 2022

Objective

Patients with COVID-19 presented with an elevated prevalence of antiphospholipid antibodies (aPL) but the relationship with thrombosis is controversial. We analysed the persistence of aPL and their association with the clinical outcomes during hospitalisation in a cohort of COVID-19 patients.

Patients and Methods

We conducted a prospective study including consecutive hospitalised patients with COVID-19 from Hospital Clínic of Barcelona between March 28th and April 22nd, 2020. Clinical outcomes during hospitalisation were thrombosis, intensive care unit (ICU) admission, and severe ventilatory failure. We determined both criteria and non-criteria aPL. Of note, in those patients with a positive result in the first determination, a second sample separated by at least 12 weeks was drawn to test the persistence of aPL.

Results

One hundred and fifty-eight patients (59.5% men) with a mean age of 61.4 ± 14.9 years old were included. Thrombosis was present in 28 (17.7%) patients, severe respiratory failure in 47 (30.5%), and 30 (18.9%) patients were admitted to ICU. Sixteen (28.6%) patients were positive for the criteria aPL at both determinations and only two (3.6%) of them suffered from thrombosis during hospitalisations (both had aCL IgG). However, they presented with low titers of aCL. Of note, aPL were not related to thrombosis, ICU admission or severe respiratory failure.

Conclusion

Although aPL were prevalent in our cohort of hospitalised COVID-19 patients and they were persistent in half of tested patients, most determinations were at low titers and they were not related to worse clinical outcomes.
Designing a Large Scale Autonomous Observing Network: A Set Theory Approach
Authors: Byrne David , Polton Jeff , Ribeiro Joseph , Fernand Liam , Holt Jason
Published: June 2022

A well designed observing network is vital to improve our understanding of the oceans and to obtain better predictions of the future. As autonomous marine technology develops, the potential for deploying large autonomous observing systems becomes feasible. Though there are many design considerations to take into account (according to the target data use cases), a fundamental requirement is to take observations that capture the variability at the appropriate length scales. In doing so, a balance must be struck between the limited observation resources available and how well they are able to represent different areas of the ocean. In this paper we present and evaluate a new method to aid decision makers in designing near-optimal observing networks. The method uses ideas from set theory to recommend an irregular network of observations which provides a guaranteed level of representation (correlation) across a domain. We show that our method places more observations in areas with smaller characteristic length scales and vice versa, as desired. We compare the method to two other grid types: regular and randomly allocated observation locations. Our new method is able to provide comparable average representation of data across the domain, whilst efficiently targeting resource to regions with shorter length scale and thereby elevating the minimum skill baseline, compared to the other two grid types. The method is also able to provide a network that represents up to 15% more of the domain area. Assessing error metrics such as Root Mean Square Error and correlation shows that our method is able to reconstruct data more consistently across all length scales, especially at smaller scales where we see RMSE 2-3 times lower and correlations of over 0.2 higher. We provide an additional discussion on the variability inherent in such methods as well as practical advice for the user. We show that considerations must be made based on time filtering, seasonality, depth and horizontal resolution.
Remarkable Natural Biological Resource of Algae for Medical Applications
Authors: Dai Na , Wang Qiang , Xu Baisheng , Chen Hui
Published: June 2022

With increasing consumer awareness of the use of natural products in pharmaceuticals and medicine, it is noted that algae can be considered an appropriate source. Algae produce many bioactive components, which have application potential in pharmaceutical industries, such as fatty acids, phycobiliprotein, polysaccharides, phenolic compounds, carotenoids, and so on. However, it is still a long way before the truly widespread application of algae in medicine, and some research and technical bottlenecks still need to be resolved for further practical use. Here, we provide an in-depth review of the current understanding of algal-based medical application, with a focus on the main pharmaceutical activity and current application stage includingin vitro, animal, and clinical studies. Furthermore, we propose some possible solutions to the obstacles that should be overcome for achieving the practical applications of algal-based medicine. Notably, animal and clinical studies on algal drugs and treatments should continue to push forward and expand for promoting the practical applications. Moreover, the developments in interdisciplinary research of algal biology and other disciplines provide new insight for driving algae-based medical application.
Radar Cross Section Approach in Illusion Effects of Transformation Optics-Based Expander
Authors: Sadeghi Mohammad Mehdi
Published: June 2022

In this paper, analytical calculation has been provided to show illusion perception in electromagnetics expander. For this end, a precise analytical solution has been done for a scattered wave from the expander including an object in the core medium. Also, this analytical calculation has been done for a bare transformed object with different size and constitute parameters (CPs). Illusion perception, in the far field, can be illustrated by comparing the calculated scattered field patterns (SFP) of the object placed inside the expander with SFP of the bare transformed object. Moreover, the same calculation and comparison has been done for nearfield SFP. In continuation, for precise deduction, radar cross sections (RCSs) of both objects have been calculated and plotted using MATLAB. Well functionality in illusion perception has been obtained using comparisons in both analytical SFPs parts and RCSs parts.
Efficacy of N-acetylcysteine in Preventing Acute Kidney Injury and Major Adverse Cardiac Events After Cardiac Surgery: A Meta-Analysis and Trial Sequential Analysis
Authors: Zhao Jingtao , Li Maowei , Tan Chen
Published: June 2022

Background

The effect of N-acetylcysteine (NAC), an antioxidant, on preventing acute kidney injury (AKI) and major adverse cardiac events (MACE) remains controversial. Therefore, we conducted this meta-analysis and trial sequential analysis to evaluate its efficacy on cardiac surgery-related adverse events.

Methods

PubMed, Embase, and Cochrane Library were searched for relevant studies from inception to June 2021. We selected randomized controlled trials comparing NAC with controls in patients undergoing cardiac surgery.

Results

Twenty-five studies including 2,444 patients met the inclusion criteria. The pooled results showed that there was no significant difference in the incidence of AKI between the NAC and control groups [relative risk (RR) = 0.91, 95% confidence interval (CI) = 0.77, 1.08, P = 0.28], but the trial sequential analysis (TSA) could not confirm this result. No difference was observed in the need for renal replacement therapy (RRT), all-cause mortality, MACE, length of stay in the intensive care unit (ICU), and length of stay in the hospital. Results of subgroup analysis results showed that intravenous infusion instead of oral NAC could significantly reduce the incidence of AKI and arrhythmia (RR = 0.84, 95% CI = 0.71, 0.99,P= 0.03,I2= 3% and RR = 0.74, 95% CI = 0.61, 0.91,P= 0.004,I2= 48%, respectively).

Conclusion

Intravenous administration of NAC can reduce the incidence of AKI and arrhythmia in patients after cardiac surgery, but cannot reduce all-cause mortality, AMI, cardiac insufficiency, and the number of patients using RRT. Oral NAC has no significant effect on the outcomes of patients after cardiac surgery.
A Multicenter Real-World Study Evaluating the Hepatoprotective Effect of Polyene Phosphatidylcholine Against Chronic Hepatitis B
Authors: Xu Jinghang , Fan Yanan , Yu Yanyan , Han Yifan , Kang Qian , Tan Ning , Yang Yuqing , Chen Hongyu , Pan Jiali , Xu Xiaoyuan
Published: June 2022

Background

Polyene phosphatidylcholine (PPC) has been widely used to treat liver diseases in China. However, there is a lack of post-marketing evidence demonstrating its liver-protective efficiency among patients infected with hepatitis B virus (HBV). This study analyzed the multicenter real-world data to compare the effectiveness of PPC with those of magnesium isoglycyrrhizinate (IsoMag) and glutathione (GSH) in patients with liver injury.

Methods

This study comprised the real-world data analysis of a multicenter, retrospective observational cohort. The data were retrieved from the Cooperative Registry of the Hospital Prescription in China between 1 October 2018, and 30 September 2019. A growth curve analysis was performed to compare the effects of different treatments on liver function longitudinally for up to 30 days after treatment commencement. In addition, the dose effect of the PPC treatment was investigated.

Results

The final cohort included 6,052 patients with approximately 8% infected with HBV (N = 471). There were 1,649, 1,750, and 2,653 patients in the PPC, GSH, and IsoMag groups, respectively, with an average age of 53.9 years. In patients with HBV infection, the PPC treatment was associated with a significant decline in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels (slopes: −3.7, 95% CI, −6.0 to −1.5 U/L/day; −2.4, 95% CI, −4.5 to −0.3 U/L/day, respectively). However, there were no significant differences in the effects among the three groups. In patients without HBV infection, the PPC treatment decreased ALT, AST, γ-glutamyl transferase (GGT), and albumin levels (−5.2, 95% CI, −5.8 to −4.5 U/L/day; −3.5, 95% CI, −4.2 to −2.7 U/L/day; −4.9, 95% CI, −6.2 to −3.7 U/L/day, −0.07, 95% CI, −0.09 to −0.04 g/L/day, respectively) and showed a stronger effect on lowering ALT levels than GSH (−2.6, 95% CI, −3.3 to −1.8 U/L/day, p < 0.05), as well as a stronger effect on lowering GGT levels than IsoMag (−1.4, 95% CI, −2.4 to −0.4 U/L/day, p < 0.05). PPC had no impact on prothrombin activity levels in patients with or without HBV infection. High-dose PPC exhibited a stronger effect on lowering ALT and AST levels than low-dose PPC.

Conclusion

This was the first real-world multicenter study to demonstrate that PPC efficiently lowers ALT and AST levels in patients with liver diseases regardless of the status of HBV infection. PPC treatment showed a comparable or better effect compared with GSH and IsoMag treatments. High-dose PPC resulted in a stronger effect than low-dose PPC.
Coronavirus Disease 2019 Vaccinations in Patients With Chronic Liver Disease and Liver Transplant Recipients: An Update
Authors: Sripongpun Pimsiri , Pinpathomrat Nawamin , Bruminhent Jackrapong , Kaewdech Apichat
Published: June 2022

Coronavirus disease 2019 (COVID-19) is a current global pandemic associated with an increased mortality, particularly in patients with comorbidities. Patients with chronic liver disease (CLD) and liver transplant (LT) recipients are at higher risk of morbidity and mortality after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Many liver societies have recommended that these patients should receive COVID-19 vaccinations, although there are limited studies assessing risks and benefits in this population. In addition, two doses of mRNA vaccines may not provide sufficient immune response, and booster dose(s) may be necessary, especially in LT recipients. Notably, variants of concern have recently emerged, and it remains unclear whether currently available vaccines provide adequate and durable protective immunity against these novel variants. This review focuses on the role of COVID-19 vaccinations in CLD and LT recipients.
Effect of Plant Growth-Promoting Bacteria on Biometrical Parameters and Antioxidant Enzymatic Activities of Lupinus albus var. Orden Dorado Under Mercury Stress
Authors: Robas Mora Marina , Jiménez Gómez Pedro Antonio , González Reguero Daniel , Probanza Lobo Agustín
Published: June 2022

Heavy metal contamination of soils is a large-scale environmental problem. It leads to significant disqualification of the territory, in addition to being a source of the potential risk to human health. The exposure of plants to mercury (Hg) generates responses in its growth and their oxidative metabolism. The impact of increasing concentrations of Hg on the development of Lupinus albus var. Orden Dorado seedlings has been studied, as well as the plant’s response to the maximum concentration of Hg that allows its development (16 μg ml–1). The result shows that only the inoculum with plant growth promoting bacteria (PGPB) allows the biometric development of the seedling (root length, weight, and number of secondary roots) and prevents the toxic effects of the heavy metal from aborting the seedlings. Specifically, treatments with strains 11, 20 (Bacillus toyonensis), 48 (not determined), and 76 (Pseudomonas syringae) are interesting candidates for further PGPB-assisted phytoremediation trials as they promote root biomass development, through their PGPB activities. The plant antioxidant response has been analyzed by quantifying the catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR) enzyme activity in the root, under 16 μg ml–1 of HgCl2 and different PGPB treatments. Results show that, although Hg stress generally induces enzyme activity, strains 31 and 69I (Pseudomonas corrugata) and 18 and 43 (Bacillus toyonensis) can keep SOD and APX levels close to those found in control without Hg (p < 0.01). Strain 18 also shows a significant reduction of GR to control levels without Hg. The present work demonstrates the benefit of PGPB treatments in situations of high Hg stress. These findings may be a good starting point to justify the role of PGPB naturally isolated from bulk soil and the rhizosphere of plants subjected to high Hg pressure in plant tolerance to such abiotic stress conditions. More studies will be needed to discover the molecular mechanisms behind the phytoprotective role of the strains with the best results, to understand the complex plant-microorganism relationships and to find effective and lasting symbioses useful in bioremediation processes.
Fungal Diversity in Barley Under Different Storage Conditions
Authors: Cao Dongmei , Lou Yuhao , Jiang Xiujie , Zhang Dongjie , Liu Junmei
Published: June 2022

The diversity of fungi in barley in simulated storage environments was analyzed. Barley was stored at different temperatures (15, 25, 35°C) and relative humidity (55, 65, 75, 85 RH) for 180 and 360 days. Alpha diversity, beta diversity, species composition, and species differences were analyzed using Illumina HiSeq technology. The fungal communities in all barley samples before and after storage belonged to 3 phyla, 18 classes, 39 orders, 71 families, 103 genera, and 152 species. The relative abundance of the dominant phylum Ascomycota was 77.98–99.19%. The relative abundance of Basidiomycota was 0.77–21.96%. At the genus level, the dominant genera of fungi in barley initially included Fusarium, Aspergillus, Microdochium, Alternaria, and Epicoccum. After 360 days of storage, the dominant genera became Epicoccum, Alternaria, Bipolar, Cladosporium, Fusarium, and Aspergillus. According to Venn diagrams and principal coordinates analysis, the fungal community diversity in barley initially was much higher than in barley stored at different temperatures and humidity. The application of PLS-DA could accurately distinguish between barley stored for 180 and 360 days. Some high-temperature and high-humidity environments accelerated storage. The dominant genera differed in different storage conditions and constantly changed with increasing storage duration. Epicoccum was one of the dominant genera after longer storage periods. This study provides theoretical support for optimizing safe storage conditions in barley.
Comparative Effect of Commercially Available Nanoparticles on Soil Bacterial Community and “Botrytis fabae” Caused Brown Spot: In vitro and in vivo Experiment
Authors: Sabra Mayada A. , Alaidaroos Bothaina A. , Jastaniah Samyah D. , Heflish Ahmed I. , Ghareeb Rehab Y. , Mackled Marwa I. , El-Saadony Mohamed T. , Abdelsalam Nader R. , Conte-Junior Carlos Adam
Published: June 2022

This study revealed the possible effects of various levels of silver nanoparticle (AgNP) application on plant diseases and soil microbial diversity. It investigated the comparison between the application of AgNPs and two commercial nanoproducts (Zn and FeNPs) on the rhizobacterial population and Botrytis fabae. Two experiments were conducted. The first studied the influence of 13 AgNP concentration on soil bacterial diversity besides two other commercial nanoparticles, ZnNPs (2,000 ppm) and FeNPs (2,500 ppm), used for comparison and application on onion seedlings. The second experiment was designed to determine the antifungal activity of previous AgNP concentrations (150, 200, 250, 300, 400, and 500 ppm) against B. fabae, tested using commercial fungicide as control. The results obtained from both experiments revealed the positive impact of AgNPs on the microbial community, representing a decrease in both the soil microbial biomass and the growth of brown spot disease, affecting microbial community composition, including bacteria, fungi, and biological varieties. In contrast, the two commercial products displayed lower effects compared to AgNPs. This result clearly showed that the AgNPs strongly inhibited the plant pathogen B. fabae growth and development, decreasing the number of bacteria (cfu/ml) and reducing the rhizosphere. Using AgNPs as an antimicrobial agent in the agricultural domain is recommended.
Age-Related Decline of Speech Perception
Authors: Hoppe Ulrich , Hocke Thomas , Iro Heinrich
Published: June 2022

Hearing loss is one of the most common disorders worldwide. It affects communicative abilities in all age groups. However, it is well known that elderly people suffer more frequently from hearing loss. Two different model approaches were employed: A generalised linear model and a random forest regression model were used to quantify the relationship between pure-tone hearing loss, age, and speech perception. Both models were applied to a large clinical data set of 19,801 ears, covering all degrees of hearing loss. They allow the estimation of age-related decline in speech recognition for different types of audiograms. Our results show that speech scores depend on the specific type of hearing loss and life decade. We found age effects for all degrees of hearing loss. A deterioration in speech recognition of up to 25 percentage points across the whole life span was observed for constant pure-tone thresholds. The largest decrease was 10 percentage points per life decade. This age-related decline in speech recognition cannot be explained by elevated hearing thresholds as measured by pure-tone audiometry.