Effect of Inositol on Lipid Peroxidation and Morphological Changes in the Eyes of Different Sexes Animals with Metabolic Associated Fatty Liver Disease
Authors: Levchuk Oksana , Denefil Olha 
Published: December 2024

Introduction. Steatotic liver disease associated with metabolic dysfunction is characterized by hepatic steatosis and at least one cardiometabolic risk factor without any other identifiable cause. Their prevalence is increasing year by year. Changes associated with hepatocyte damage, inflammation, and fibrosis are observed in the liver. These diseases are often complicated by concomitant metabolic diseases, including obesity, type 2 diabetes and hypertension, metabolic disorders in various organs, including the eye. At the same time, the integrity of cells is violated, the processes of free radical oxidation of lipids and proteins are activated. There is data in the literature that inositols are used to treat steatotic liver disease associated with metabolic dysfunction.
The aim of the research: determine the features of changes in the content of lipid peroxidation products in the homogenates of the eyes of rats with experimentally simulated metabolically associated steatotic liver disease (MASLC) and the influence of inositol isoforms on their content and to evaluate the morphological changes in the eyes under these conditions.
Research methods. Experiments were performed on 216 white rats of different sexes, at the time of withdrawal from the experiment they were 7.5-8 months old. The animals were divided into 9 groups: 1 – control, 2 – myo-inositol (MI), 3 – D-chiro inositol (DI), 4 – MASLP 2 months, 5 – MASLP 2 months + MI 2 months, 6 – MASLP 2 months + DI 2 months, 7 – MASLP 4 months, 8 – MASLP 4 months+ MI 2 last months, 9 – MASLP 4 months+DI 2 last months. MASLP was induced by giving 20% fructose solution (Fr) instead of drinking for 2 months in groups 4. 5 and 6, for 4 months in groups 7, 8 and 9. Inositols were administered intragastrically (MI – 400 mg/kg of weight, DI – 30 mg/kg of weight) after 2 months from the beginning of the simulation of MASHP. The content of diene and triene conjugates (DC, TC), Schiff's bases (SB), TBA-active products (TBA-ap), superoxide dismutase (SOD) and catalase activity (CAT) was determined in eye homogenates. A morphological study of the eyes was carried out.
Results and discussion. An increase in DC, TC, SB, TBA-ap, SOD, and CAT was revealed in males and females with MASLP, which were higher in group 7, compared to group 4. excluding CAT in females. MI and DI had a corrective effect, and males had a greater effect in group 7 (there were also significantly higher SOD and CAT), and females - in group 4.
Conclusions. 1. With experimentally modeled MASLP in the eyes, the processes of lipid peroxidation are activated, DC, TC, SB, TBA-ap, as well as SOD and CAT increase. Longer consumption of Fr causes more damage. With 2-month use of Fr, the changes are greater in males. 2. Correction of the simulated MASLP MI and DI with 2 and 4 monthes use of Fr causes a decrease in the products of lipid peroxidation and antioxidants in eye homogenates, which is more pronounced with 4 monthes use of Fr in males, and with 2 monthes use in females. 3. The morphological study showed a significant violation of the retina of the eyes with experimentally simulated MASLP, which is more pronounced with longer use of Fr. MI and DI have a corrective effect on the structure of the retina.