bluuebunny/crossref_metadata_2025 huggingface dataset:
Disturbance Rejection Control Method for Isolated Three-Port Converter with Virtual Damping
Authors: You Jiang , Liao Mengyan , Chen Hailong , Ghasemi Negareh , Vilathgamuwa Mahinda
Published: November 2018

The high-power density and capability of three-port converters (TPCs) in generating demanded power synchronously using flexible control strategy make them potential candidates for renewable energy applications to enhance efficiency and power density. The control performance of isolated TPCs can be degraded due to the coupling and interaction of power transmission among different ports, variations of model parameters caused by the changes of the operation point and resonant peak of LC circuit. To address these issues, a linear active disturbance rejection control (LADRC) system is developed in this paper for controlling the utilized TPC. A virtual damping based method is proposed to increase damping ratio of current control subsystem of TPC which is beneficial in further improving dynamic control performance. The simulation and experimental results show that compared to the traditional frequency control strategy, the control performance of isolated TPC can be improved by using the proposed method.
Landscape Topoedaphic Features Create Refugia from Drought and Insect Disturbance in a Lodgepole and Whitebark Pine Forest
Authors: Cartwright Jennifer
Published: November 2018

Droughts and insect outbreaks are primary disturbance processes linking climate change to tree mortality in western North America. Refugia from these disturbances—locations where impacts are less severe relative to the surrounding landscape—may be priorities for conservation, restoration, and monitoring. In this study, hypotheses concerning physical and biological processes supporting refugia were investigated by modelling the landscape controls on disturbance refugia that were identified using remotely sensed vegetation indicators. Refugia were identified at 30-m resolution using anomalies of Landsat-derived Normalized Difference Moisture Index in lodgepole and whitebark pine forests in southern Oregon, USA, in 2001 (a single-year drought with no insect outbreak) and 2009 (during a multi-year drought and severe outbreak of mountain pine beetle). Landscape controls on refugia (topographic, soil, and forest characteristics) were modeled using boosted regression trees. Landscape characteristics better explained and predicted refugia locations in 2009, when forest impacts were greater, than in 2001. Refugia in lodgepole and whitebark pine forests were generally associated with topographically shaded slopes, convergent environments such as valleys, areas of relatively low soil bulk density, and in thinner forest stands. In whitebark pine forest, refugia were associated with riparian areas along headwater streams. Spatial patterns in evapotranspiration, snowmelt dynamics, soil water storage, and drought-tolerance and insect-resistance abilities may help create refugia from drought and mountain pine beetle. Identification of the landscape characteristics supporting refugia can help forest managers target conservation resources in an era of climate-change exacerbation of droughts and insect outbreaks.
Converting Larch Plantations to Larch-Walnut Mixed Stands: Effects of Spatial Distribution Pattern of Larch Plantations on the Rodent-Mediated Seed Dispersal of Juglans mandshurica
Authors: Wang Jing , Yan Qiaoling , Zhang Ting , Lu Deliang , Xie Jin , Sun Yirong , Zhang Jinxin , Zhu Jiaojun
Published: November 2018

Larch (Larix spp.) is planted after a clear-cut of secondary forests (SFs) to meet the timber demand of Northeast China. However, the declination of soil fertility and the acidification of surface runoff in larch plantations (LPs) resulting from mono-species composition have threatened the sustainable development of LPs. Converting pure LPs into larch-walnut mixed forests can solve those problems, in which it is crucial to promote the seed regeneration of Juglans mandshurica in LPs. The success of walnut seed regeneration is dependent on rodent removing seed away from mother trees and the dispersal processes rely on the stand structure. The spatial distribution pattern between LPs and SFs is a type of stand structure that might affect seed dispersal. There are two typical spatial patterns in Northeast China due to different topographical conditions, that is, contour type (C-T, LPs and SFs located at the same slope position and aspect) and downslope type (D-T, LPs located at the down slope of the adjacent SF in the same aspect). The objectives of our study were to verify the effects of the spatial distribution pattern of LPs on rodent-mediated seed dispersal and to determine the optimal spatial distribution type for seed dispersal. The field trial was conducted by releasing tagged J. mandshurica seeds at three stages of seed rain during two continuous growing seasons in two types of spatial distribution patterns for LPs. We found that contour type had a substantial advantage in the rodent-mediated seed dispersal, that is, the dispersal rate and the proportion of scatter-hoarded seeds in LPs of C-T stands were much higher than in D-T stands, respectively. These differences may be caused by the variation in the topographic factor for these two types of spatial distribution pattern of LPs. In the C-T stands, it was a translational motion for rodents to remove walnuts from SFs to LPs, which would be an energy efficient and favorable method of seed dispersal. Fluctuation of seed abundance had an effect on rodent behaviors and seed fates, that is, there was a faster dispersal rate and lower proportion of seeds in situ in the non-masting year of 2015 than in the masting year of 2016 and proportion of scatter-hoarded seeds reached the highest during the early stage and lowest at the middle stage of seed rain. Thus, the contour type of LPs with a favorable terrain advantage is potentially conducive to dispersing walnut seeds by rodents during early stage of seed rain and converting larch plantations into larch-walnut mixed forests.
Microstreaming and Its Role in Applications: A Mini-Review
Authors: Jalal Javeria , Leong Thomas S. H.
Published: November 2018

Acoustic streaming is the steady flow of a fluid that is caused by the propagation of sound through that fluid. The fluid flow in acoustic streaming is generated by a nonlinear, time-averaged effect that results from the spatial and temporal variations in a pressure field. When there is an oscillating body submerged in the fluid, such as a cavitation bubble, vorticity is generated on the boundary layer on its surface, resulting in microstreaming. Although the effects are generated at the microscale, microstreaming can have a profound influence on the fluid mechanics of ultrasound/acoustic processing systems, which are of high interest to sonochemistry, sonoprocessing, and acoustophoretic applications. The effects of microstreaming have been evaluated over the years using carefully controlled experiments that identify and quantify the fluid motion at a small scale. This mini-review article overviews the historical development of acoustic streaming, shows how microstreaming behaves, and provides an update on new numerical and experimental studies that seek to explore and improve our understanding of microstreaming.
Transgenerational Transmission of Holocaust Trauma and Its Expressions in Literature
Authors: Nir Bina
Published: November 2018

Trauma is a central concept in the historiography of the Holocaust. In both the historiographical and the psychoanalytical research on the subject, the Holocaust is perceived not as a finite event that took place in the past, but as one that continues to exist and to affect the families of survivors and the Jewish people. In the 1950s–1960s, evidence began emerging that Holocaust trauma was not limited to the survivors themselves, but was passed on to the next generation born after the Holocaust and raised in its shadow. It is possible to see the effects of growing up in the shadow of the Holocaust and transgenerational transmission of trauma in many aspects of the second-generation children’s lives. In this article, I examine the representations of these symptoms in David Grossman’s novel See Under: Love, which deals with the subject of the Holocaust through the perspective of Momik, a child of Holocaust survivors. Grossman teaches us that writing itself has the potential to heal. He also shows us that every one of us contains both victim and aggressor, and that, under certain circumstances, the “Nazi beast” may awaken within each of us.
Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps
Authors: Guo Cheng , Xie Yue , Liu Yuchen , Wang Ning , Zhan Jiafei , Zhou Xuan , Angel Christiana , Gu Xiaobin , Lai Weimin , Peng Xuerong , Yang Guangyou
Published: November 2018

Coenurus cerebralis, the metacestode of Taenia multiceps, causes coenurosis, a disease severely affecting goat, sheep, cattle and yak farming and resulting in huge economic losses annually. Annexins bind calcium ions and play an important role in flatworm parasite development. To explore potential functions of annexins in T. multiceps, three homologous genes, namely, TmAnxB2, TmAnxB3 and TmAnxB12, were screened from the transcriptome dataset, amplified from C. cerebralis cDNA and subjected to bioinformatics analysis. Then, polyclonal antibodies recognizing the recombinant TmAnxB2 (rTmAnxB2) and rTmAnxB3 were prepared for localization of TmAnxB2 and TmAnxB3 in different tissues and developmental stages by immunofluorescence. The transcription of all three genes was also measured by relative fluorescent quantitative PCR. The sizes of rTmAnxB2, rTmAnxB3 and rTmAnxB12 were 58.00, 53.06 and 53.51 kDa, respectively, and rTmAnxB12 was unstable. Both rTmAnxB2 and rTmAnxB3 were recognized by goat-positive T. multiceps sera in Western blots. Immunofluorescence revealed that TmAnxB2 and TmAnxB3 were localized in the protoscolex and cyst wall and TmAnxB3 was also detected in adult cortex. TmAnxB2 and TmAnxB12 mRNA levels were determined to be highest in oncospheres and protoscolex, whereas transcription of TmAnxB3 was highest in scolex and immature segments. Taken together, these findings indicate that TmAnxB2 and TmAnxB12 may play critical roles in T. multiceps larvae, while TmAnxB3 may have important functions in adults. These results will lay the foundation for functional research of annexins in T. multiceps.
The Plant Circadian Clock and Chromatin Modifications
Authors: Yang Ping , Wang Jianhao , Huang Fu-Yu , Yang Songguang , Wu Keqiang
Published: November 2018

The circadian clock is an endogenous timekeeping network that integrates environmental signals with internal cues to coordinate diverse physiological processes. The circadian function depends on the precise regulation of rhythmic gene expression at the core of the oscillators. In addition to the well-characterized transcriptional feedback regulation of several clock components, additional regulatory mechanisms, such as alternative splicing, regulation of protein stability, and chromatin modifications are beginning to emerge. In this review, we discuss recent findings in the regulation of the circadian clock function in Arabidopsis thaliana. The involvement of chromatin modifications in the regulation of the core circadian clock genes is also discussed.
Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii
Authors: Gomez Miguel , Leung Whinkie , Dantuluri Swathi , Pillai Alexander , Gani Zyan , Hwang Sungmin , McMillan Lana J. , Kiljunen Saija , Savilahti Harri , Maupin-Furlow Julie A.
Published: November 2018

Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced tolerance of halophilic archaea to redox-active compounds using Haloferax volcanii as a model organism. The strains were isolated from a library of random transposon mutants for growth on high doses of sodium hypochlorite (NaOCl), an agent that forms hypochlorous acid (HOCl) and other redox acid compounds common to aqueous environments of high concentrations of chloride. The transposon insertion site in each of twenty isolated clones was mapped using the following: (i) inverse nested two-step PCR (INT-PCR) and (ii) semi-random two-step PCR (ST-PCR). Genes that were found to be disrupted in hypertolerant strains were associated with lysine deacetylation, proteasomes, transporters, polyamine biosynthesis, electron transfer, and other cellular processes. Further analysis revealed a ΔpsmA1 (α1) markerless deletion strain that produces only the α2 and β proteins of 20S proteasomes was hypertolerant to hypochlorite stress compared with wild type, which produces α1, α2, and β proteins. The results of this study provide new insights into archaeal tolerance of redox active compounds such as hypochlorite.
Narrating Pregnancy and Childbirth: Infanticide and the Dramatization of Reproductive Knowledge
Authors: Steinway Elizabeth
Published: November 2018

In early modern England, infanticide was a crime overwhelmingly associated with women. Both popular texts and legal records depict women accused of infanticide as mothers acting against nature. These figures, however, do not often appear in the period’s drama. Instead, early modern drama includes fictionalized mothers who kill their children beyond infancy and into adulthood. By eschewing portrayals of neonaticide and the trials associated with it, the drama highlights a dependency upon female characters’ verbal narratives of the reproductive body that reinforces pregnancy’s unstable epistemology. I argue that the flexibility of this epistemology allows women, whether female characters in drama or historical women on trial, to distance themselves from the crime of infanticide by reconstructing narratives of both pregnancy and childbirth. Sharing rhetorical devices with the testimonies of women accused of infanticide, dramatic mothers such as Videna in The Tragedie of Gorboduc and Brunhalt in Thierry and Theodoret linguistically sever the biological ties between mother and child, thus disrupting conventional portrayals of reproduction. These parallel strategies position the reproductive female body as a site of resistance to the legal mechanisms designed to interpret it.
Molecular Mechanism for the Regulation of ABA Homeostasis During Plant Development and Stress Responses
Authors: Ma Yanlin , Cao Jing , He Jiahan , Chen Qiaoqiao , Li Xufeng , Yang Yi
Published: November 2018

The plant hormone abscisic acid (ABA) play essential roles in numerous physiological processes such as seed dormancy, seed germination, seeding growth and responses to biotic and abiotic stresses. Such biological processes are tightly controlled by a complicated regulatory network including ABA homoeostasis, signal transduction as well as cross-talking among other signaling pathways. It is known that ABA homoeostasis modulated by its production, inactivation, and transport pathways is considered to be of great importance for plant development and stress responses. Most of the enzymes and transporters involved in ABA homoeostasis have been largely characterized and they all work synergistically to maintain ABA level in plants. Increasing evidence have suggested that transcriptional regulation of the genes involved in either ABA production or ABA inactivation plays vital roles in ABA homoeostasis. In addition to transcription factors, such progress is also regulated by microRNAs and newly characterized root to shoot mobile peptide-receptor like kinase (RLKs) mediated long-distance signal transduction. Thus, ABA contents are always kept in a dynamic balance. In this review, we survey recent research on ABA production, inactivation and transport pathways, and summarize some latest findings about the mechanisms that regulate ABA homoeostasis.
Adaptation Mechanism of Salt Excluders under Saline Conditions and Its Applications
Authors: Chen Min , Yang Zhen , Liu Jing , Zhu Tingting , Wei Xiaocen , Fan Hai , Wang Baoshan
Published: November 2018

Global soil salinization is increasingly a serious threat to agriculture worldwide. Therefore, it is imperative to improve crop salt tolerance as a means of adaptation to saline habitats. Some halophytes and most monocotyledonous crops are salt-excluders. Understanding the regulatory mechanisms of salt exclusion at the molecular level in salt-exclusion plants is critical for improving the salt tolerance of monocotyledonous crops such as maize, wheat, rice, and sorghum. In this review, we summarize recent research into salt-exclusion mechanisms and the genes that underlie them. Findings related to salt exclusion may accelerate the process of breeding tolerant cultivars by using genomic and molecular tools.
Visualising Business Data: A Survey
Authors: Roberts Richard C. , Laramee Robert S.
Published: November 2018

A rapidly increasing number of businesses rely on visualisation solutions for their data management challenges. This demand stems from an industry-wide shift towards data-driven approaches to decision making and problem-solving. However, there is an overwhelming mass of heterogeneous data collected as a result. The analysis of these data become a critical and challenging part of the business process. Employing visual analysis increases data comprehension thus enabling a wider range of users to interpret the underlying behaviour, as opposed to skilled but expensive data analysts. Widening the reach to an audience with a broader range of backgrounds creates new opportunities for decision making, problem-solving, trend identification, and creative thinking. In this survey, we identify trends in business visualisation and visual analytic literature where visualisation is used to address data challenges and identify areas in which industries use visual design to develop their understanding of the business environment. Our novel classification of literature includes the topics of businesses intelligence, business ecosystem, customer-centric. This survey provides a valuable overview and insight into the business visualisation literature with a novel classification that highlights both mature and less developed research directions.
Capital Adequacy, Deposit Insurance, and the Effect of Their Interaction on Bank Risk
Authors: Jumreornvong Seksak , Chakreyavanich Chanakarn , Treepongkaruna Sirimon , Jiraporn Pornsit
Published: November 2018

This paper investigates how deposit insurance and capital adequacy affect bank risk for five developed and nine emerging markets over the period of 1992–2015. Although full coverage of deposit insurance induces moral hazard by banks, deposit insurance is still an effective tool, especially during the time of crisis. On the contrary, capital adequacy by itself does not effectively perform the monitoring role and leads to the asset substitution problem. Implementing the safety nets of both deposit insurance and capital adequacy together could be a sustainable financial architecture. Immediate-effect analysis reveals that the interplay between deposit insurance and capital adequacy is indispensable for banking system stability.
Reduction of CO2 Emissions and Cost Analysis of Ultra-Low Viscosity Engine Oil
Authors: Ishizaki Keita , Nakano Masaru
Published: November 2018

This study is focused on the reduction of CO2 emissions and costs associated with ultra-low viscosity (ULV) engine oils for passenger vehicles. Specifically, the reduction in life cycle CO2 (LCCO2) emissions from lower-viscosity engine oil and the oil drain interval (ODI) extension were estimated taking into account both mineral engine oil and synthetic engine oil. Furthermore, the cost-effectiveness of ULV engine oils were investigated by performing base-stock cost analysis. When the volatility limit of the Noack test (American Society for testing and materials (ASTM) D5800) was set to 15 wt %, the results indicated that the lower limit of kinematic viscosity at 100 °C (KV100) for mineral engine oil (with Group-III base-stock) and synthetic engine oil (with polyalphaolefin (PAO) base-stock) were approximately 5.3 and 4.5 mm2/s, respectively. Compared to conventional 0W-16 mineral engine oil (KV100 6.2 mm2/s), the effect of reducing LCCO2 emissions on ULV mineral engine oil (ULV-Mineral, KV100 5.3 mm2/s) was estimated at 0.6%, considering 1.5–1.8 L gasoline engines in New European Driving Cycles (NEDC) mode. ULV-Mineral, which continues to use a mineral base-stock, is considered highly cost-effective since its cost is similar to the conventional 0W-16 mineral engine oil. On the other hand, compared with ULV-Mineral, the vehicle fuel efficiency improvement from the use of ULV synthetic engine oil (ULV-PAO, KV100 4.5 mm2/s) was estimated to be 0.5%. However, considering CO2 emissions during engine oil production, the reduction of LCCO2 emission from ULV-PAO compared with ULV-Mineral was estimated to be only 0.1% or less using 2030 standards (assuming a vehicle fuel efficiency of 66.5 g-CO2/km) when ODI is set equivalent (7500 km) to mineral engine oil. As a result, ULV-PAO’s cost-effectiveness, considering the cost increase of PAO base-stock, was found to be nominal. Contrariwise, when the characteristics of PAO base-stock with higher oxidation stability are used comparatively with the mineral base-stock while extending the ODI to 15,000 km, the effect of reducing LCCO2 emissions of ULV-PAO was estimated to be 0.7% in 2030, making ULV-PAO a competitive and cost-effective alternative. In other words, the popularization of synthetic engine oil toward 2030 will require the consideration of both viscosity reduction and ODI extension.
A Facile and Low-Cost Method to Produce Ultrapure 99.99999% Gallium
Authors: Pan Kefeng , Li Ying , Zhang Jiawei , Zhao Qing
Published: November 2018

As one of the critical raw materials, very pure gallium is important for the semiconductor and photoelectric industry. Unfortunately, refining gallium to obtain a purity that exceeds 99.99999% is very difficult. In this paper, a new, facile and efficient continuous partial recrystallization method to prepare gallium of high purity is investigated. Impurity concentrations, segregation coefficients, and the purification effect were measured. The results indicated that the contaminating elements accumulated in the liquid phase along the crystal direction. The order of the removal ratio was Cu > Mg > Pb > Cr > Zn > Fe. This corresponded to the order of the experimentally obtained segregation coefficients for each impurity: Cu < Mg < Pb < Cr < Zn < Fe. The segregation coefficient of the impurities depended strongly on the crystallization rate. All observed impurity concentrations were substantially reduced, and the purity of the gallium obtained after our refinement exceeded 99.99999%.
One-Step Preparation of Super-Hydrophobic Micro-Nano Dendrites on Al Alloy for Enhanced Corrosion Resistance
Authors: Li Xuewu , Shi Tian , Li Ben , Zhang Chuanwei , Zhong Bin , Lv Yuan , Zhang Qiaoxin
Published: November 2018

Corrosion failure is a thorny issue that restricts the applications of Al alloys. As a research hotspot in functional realization, hydrophobic fabrication exactly offers an efficient method to settle metallic corrosions. This work has developed a facile and low-cost method to enhance corrosion resistance of Al alloys. The micro-nano dendrites have been firstly prepared on metallic substrate using one-step potentiostatic deposition. Then, wetting and electrochemical behaviors have been systematically investigated after stearic acid modification. Results show that the as-prepared surface possesses amplified and durable water repellence with an apparent contact angle (CA) of 154.2° and a sliding angle (SA) of 4.7°. Meanwhile, owing to the trapped air in dendrites, the newly-generated solid-air-liquid interfaces help to resist seawater penetration by reducing interfacial interactions on the super-hydrophobic surface as well as significantly enhance its corrosion resistance. This work sheds positive insights into extending the applications of Al alloys in many areas, especially for ocean engineering fields.
Major Impediment to Highly Efficient, Stable and Low-Cost Perovskite Solar Cells
Authors: Zhang Yue , Zhang Haiming , Zhang Xiaohui , Wei Lijuan , Zhang Biao , Sun Yuxuan , Hai Guangyuan , Li Yujie
Published: November 2018

Organic–inorganic hybrid perovskite solar cells (PSCs) have made immense progress in recent years, owing to outstanding optoelectronic properties of perovskite materials, such as high extinction coefficient, carrier mobility, and low exciton binding energy. Since the first appearance in 2009, the efficiency of PSCs has reached 23.3%. This has made them the most promising rival to silicon-based solar cells. However, there are still several issues to resolve to promote PSCs’ outdoor applications. In this review, three crucial aspects of PSCs, including high efficiency, environmental stability, and low-cost of PSCs, are described in detail. Recent in-depth studies on different aspects are also discussed for better understanding of these issues and possible solutions.
Probability-Statistical Estimation Method of Feed Influence on As-Turned Finish of Steels and Non-Ferrous Metals
Authors: Kryvyi Petro , Dzyura Volodymyr , Tymoshenko Nadiya , Maruschak Pavlo , Nugaras Justas , Prentkovskis Olegas
Published: November 2018

Based on the experimental data on the roughness parameter Ra, which stands for the mean arithmetic deviation of the profile, as obtained in the process of turning test specimens from different materials with constant elements of the cutting mode (depth a and velocity v) and structural-geometric parameters of the cutting tool, but with different feed rates f, the probability-statistical method for estimating the influence of feed rate f on the resulting surface roughness by the parameter Ra is proposed using the theory of a small sample.
Simulation of a Mining Value Chain with a Synthetic Ore Body Model: Iron Ore Example
Authors: Lishchuk Viktor , Lund Cecilia , Lamberg Pertti , Miroshnikova Elena
Published: November 2018

Reconciliation of geological, mining and mineral processing information is a costly and time demanding procedure with high uncertainty due to incomplete information, especially during the early stages of a project, i.e., pre-feasibility, feasibility studies. Lack of information at those project stages can be overcome by applying synthetic data for investigating different scenarios. Generation of the synthetic data requires some minimum sparse knowledge already available from other parts of the mining value chain, i.e., geology, mining, mineral processing. The aim of the paper is to describe how to establish and construct a synthetic testing environment, or “synthetic ore body model” for data integration by using a synthetic deposit, mine production, constrained by a mine plan, and a simulated beneficiation process. The approach uses quantitative mineralogical data and liberation information for process simulation. The results of geological and process data integration are compared with the real case data of an apatite iron ore. The discussed approach allows for studying the implications in downstream processes caused by changes in upstream parts of the mining value chain. It also opens the possibility of optimising sampling campaigns by investigating different synthetic drilling scenarios including changes to the spacing between synthetic drill holes, composite length, drill hole orientation and assayed parameters. A synthetic deposit model can be a suitable tool for testing different scenarios for implementation of geometallurgical programs and also an educational tool for universities and companies.
DNA Replication: From Radioisotopes to Click Chemistry
Authors: Ligasová Anna , Koberna Karel
Published: November 2018

The replication of nuclear and mitochondrial DNA are basic processes assuring the doubling of the genetic information of eukaryotic cells. In research of the basic principles of DNA replication, and also in the studies focused on the cell cycle, an important role is played by artificially-prepared nucleoside and nucleotide analogues that serve as markers of newly synthesized DNA. These analogues are incorporated into the DNA during DNA replication, and are subsequently visualized. Several methods are used for their detection, including the highly popular click chemistry. This review aims to provide the readers with basic information about the various possibilities of the detection of replication activity using nucleoside and nucleotide analogues, and to show the strengths and weaknesses of those different detection systems, including click chemistry for microscopic studies.
Prediction of GluN2B-CT1290-1310/DAPK1 Interaction by Protein–Peptide Docking and Molecular Dynamics Simulation
Authors: Tu Gao , Fu Tingting , Yang Fengyuan , Yao Lixia , Xue Weiwei , Zhu Feng
Published: November 2018

The interaction of death-associated protein kinase 1 (DAPK1) with the 2B subunit (GluN2B) C-terminus of N-methyl-D-aspartate receptor (NMDAR) plays a critical role in the pathophysiology of depression and is considered a potential target for the structure-based discovery of new antidepressants. However, the 3D structures of C-terminus residues 1290–1310 of GluN2B (GluN2B-CT1290-1310) remain elusive and the interaction between GluN2B-CT1290-1310 and DAPK1 is unknown. In this study, the mechanism of interaction between DAPK1 and GluN2B-CT1290-1310 was predicted by computational simulation methods including protein–peptide docking and molecular dynamics (MD) simulation. Based on the equilibrated MD trajectory, the total binding free energy between GluN2B-CT1290-1310 and DAPK1 was computed by the mechanics generalized born surface area (MM/GBSA) approach. The simulation results showed that hydrophobic, van der Waals, and electrostatic interactions are responsible for the binding of GluN2B-CT1290–1310/DAPK1. Moreover, through per-residue free energy decomposition and in silico alanine scanning analysis, hotspot residues between GluN2B-CT1290-1310 and DAPK1 interface were identified. In conclusion, this work predicted the binding mode and quantitatively characterized the protein–peptide interface, which will aid in the discovery of novel drugs targeting the GluN2B-CT1290-1310 and DAPK1 interface.
Preparation of Enantiomeric β-(2′,5′-Dimethylphenyl)Bromolactones, Their Antiproliferative Activity and Effect on Biological Membranes
Authors: Gładkowski Witold , Włoch Aleksandra , Pawlak Aleksandra , Sysak Angelika , Białońska Agata , Mazur Marcelina , Mituła Paweł , Maciejewska Gabriela , Obmińska-Mrukowicz Bożena , Kleszczyńska Halina
Published: November 2018

Three novel enantiomeric pairs of bromolactones possesing a 2,5-dimethylphenyl substituent at the β-position of the lactone ring have been synthesized from corresponding enantiomeric (E)-3-(2′,5′-dimethylphenyl)hex-4-enoic acids (4) by kinetically controlled bromolactonization with N-bromosuccinimide (NBS). γ-Bromo-δ-lactones (5) were isolated as the major products. Absolute configurations of stereogenic centers of γ-bromo-δ-lactones (5) were assigned based on X-ray analysis; configurations of cis δ-bromo-γ-lactones (6) and trans δ-bromo-γ-lactones (7) were determined based on mechanism of bromolactonization. Synthesized compounds exhibited significant antiproliferative activity towards the four canine cancer cell lines (D17, CLBL-1, CLB70, and GL-1) and one human cancer line (Jurkat). Classifying the compounds in terms of activity, the most active were enantiomers of trans δ-bromo-γ-lactones (7) followed by enantiomers of cis isomer (6) and enantiomeric γ-bromo-δ-lactones (5). Higher activity was observed for all stereoisomers with S configuration at C-4 in comparison with their enantiomers with 4R configuration. Synthesized compounds did not induce hemolysis of erythrocytes. The results of the interaction of bromolactones with red blood cell membranes suggest that these compounds incorporate into biological membranes, concentrating mainly in the hydrophilic part of the bilayer but have practically no influence on fluidity in the hydrophobic region. The differences in interactions with the membrane between particular enantiomers were observed only for γ-lactones: stronger interactions were found for enantiomer 4R,5R,6S of cis γ-lactone (6) and for enantiomer 4S,5R,6S of trans γ-lactone (7).
Green Synthesis of Ag Nanoparticles Using Grape Stalk Waste Extract for the Modification of Screen-Printed Electrodes
Authors: Bastos-Arrieta Julio , Florido Antonio , Pérez-Ràfols Clara , Serrano Núria , Fiol Núria , Poch Jordi , Villaescusa Isabel
Published: November 2018

The chemical synthesis of silver nanoparticles (Ag-NPs) by using an environmentally friendly methodology for their preparation is presented. Thus, considering that plants possess components that can act as reducing agents and stabilizers in nanoparticles’ production, the synthesis of Ag-NPs by using an extract aqueous solution of grape stalk waste as a reducing and capping agent is studied. First, the total polyphenols and reducing sugars contained in the produced extracts at different conditions are characterized. After that, Ag-NPs are synthesized regarding the interaction of Ag ions (from silver nitrate) and the grape stalk extract. The effect of temperature, contact time, extract/metal solution volume ratio and pH solution in the synthesis of metal nanoparticles are also studied. Different sets of nanoparticle samples are characterized by means of Electron Microscopy coupled with Energy Dispersive X-Ray for qualitative chemical identification. Ag-NPs with an average diameter of 27.7 ± 0.6 nm are selected to proof their suitability for sensing purposes. Finally, screen-printed electrodes modified with Ag-NPs are tested for the simultaneous stripping voltammetric determination of Pb(II) and Cd(II). Results indicate good reproducibility, sensitivity and limits of detection around 2.7 µg L−1 for both metal ions.
Peculiarities of Synthesis and Properties of Lignin–Silica Nanocomposites Prepared by Sol-Gel Method
Authors: Budnyak Tetyana M. , Aminzadeh Selda , Pylypchuk Ievgen V. , Riazanova Anastasia V. , Tertykh Valentin A. , Lindström Mikael E. , Sevastyanova Olena
Published: November 2018

The development of advanced hybrid materials based on polymers from biorenewable sources and mineral nanoparticles is currently of high importance. In this paper, we applied softwood kraft lignins for the synthesis of lignin/SiO2 nanostructured composites. We described the peculiarities of composites formation in the sol-gel process through the incorporation of the lignin into a silica network during the hydrolysis of tetraethoxysilane (TEOS). The initial activation of lignins was achieved by means of a Mannich reaction with 3-aminopropyltriethoxysilane (APTES). In the study, we present a detailed investigation of the physicochemical characteristics of initial kraft lignins and modified lignins on each step of the synthesis. Thus, 2D-NMR, 31P-NMR, size-exclusion chromatography (SEC) and dynamic light scattering (DLS) were applied to analyze the characteristics of pristine lignins and lignins in dioxan:water solutions. X-Ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) were used to confirm the formation of the lignin–silica network and characterize the surface and bulk structures of the obtained hybrids. Termogravimetric analysis (TGA) in nitrogen and air atmosphere were applied to a detailed investigation of the thermal properties of pristine lignins and lignins on each step of modification. SEM confirmed the nanostructure of the obtained composites. As was demonstrated, the activation of lignin is crucial for the sol-gel formation of a silica network in order to create novel hybrid materials from lignins and alkoxysilanes (e.g., TEOS). It was concluded that the structure of the lignin had an impact on its reactivity during the activation reaction, and consequently affected the properties of the final hybrid materials.
pH-Sensitive Poly(β-amino ester)s Nanocarriers Facilitate the Inhibition of Drug Resistance in Breast Cancer Cells
Authors: Zhou Mengxue , Zhang Xingcai , Xie Jin , Qi Rongxiang , Lu Huiru , Leporatti Stefano , Chen Jun , Hu Yi
Published: November 2018

Multidrug resistance (MDR) remains an unmet challenge in chemotherapy. Stimuli-responsive nanocarriers emerge as a promising tool to overcome MDR. Herein, pH-sensitive poly(β-amino ester)s polymers (PHP)-based micellar nanoparticles were synthesized for enhanced doxorubicin (DOX) delivery in drug resistant breast cancer MCF-7/ADR cells. DOX-loaded PHP micelles showed rapid cell-internalization and lysosomal escape in MCF-7/ADR cells. The cytotoxicity assays showed relatively higher cell inhibition of DOX-loaded PHP micelles than that of free DOX against MCF-7/ADR cells. Further mechanistic studies showed that PHP micelles were able to inhibit P-glycoprotein (P-gp) activity by lowering mitochondrial membrane potentials and ATP levels. These results suggested that the enhanced antitumor effect might be attributed to PHP-mediated lysosomal escape and drug efflux inhibition. Therefore, PHP would be a promising pH-responsive nanocarrier for enhanced intracellular drug delivery and overcoming MDR in cancer cells.
Growth and Self-Assembly of Silicon–Silicon Carbide Nanoparticles into Hybrid Worm-Like Nanostructures at the Silicon Wafer Surface
Authors: Perez-Guzman Manuel Alejandro , Ortega-Amaya Rebeca , Matsumoto Yasuhiro , Espinoza-Rivas Andres Mauricio , Morales-Corona Juan , Santoyo-Salazar Jaime , Ortega-Lopez Mauricio
Published: November 2018

This work describes the growth of silicon–silicon carbide nanoparticles (Si–SiC) and their self-assembly into worm-like 1D hybrid nanostructures at the interface of graphene oxide/silicon wafer (GO/Si) under Ar atmosphere at 1000 °C. Depending on GO film thickness, spread silicon nanoparticles apparently develop on GO layers, or GO-embedded Si–SiC nanoparticles self-assembled into some-micrometers-long worm-like nanowires. It was found that the nanoarrays show that carbon–silicon-based nanowires (CSNW) are standing on the Si wafer. It was assumed that Si nanoparticles originated from melted Si at the Si wafer surface and GO-induced nucleation. Additionally, a mechanism for the formation of CSNW is proposed.
Serum Triglycerides and Atherosclerotic Cardiovascular Disease: Insights from Clinical and Genetic Studies
Authors: Tada Hayato , Nohara Atsushi , Kawashiri Masa-aki
Published: November 2018

Lipoproteins are a major risk factor for atherosclerotic cardiovascular diseases (ASCVD). Among the lipoproteins, low-density lipoproteins (LDL) have been shown to be causally associated with ASCVD development. In contrast, triglycerides or triglyceride-rich lipoproteins receive less attention than LDL because there is little definite evidence from randomized controlled trials. A Mendelian randomization study has recently been published in which a causal association could be estimated with observational datasets. Using such Mendelian randomization studies, ranging from common to rare genetic variations, triglycerides seem to be causally associated with ASCVD outcomes independent of LDL. Although the “causal association” of serum triglycerides and ASCVD is difficult to assert, accumulated evidence from clinical and Mendelian randomization studies, using common and rare genetic variations, strongly supports such an association. In this article, we provide a summary of investigations focusing on important causal associations between serum triglycerides and ASCVD from the clinical point of view.
Gut Permeability Might be Improved by Dietary Fiber in Individuals with Nonalcoholic Fatty Liver Disease (NAFLD) Undergoing Weight Reduction
Authors: Krawczyk Marcin , Maciejewska Dominika , Ryterska Karina , Czerwińka-Rogowska Maja , Jamioł-Milc Dominika , Skonieczna-Żydecka Karolina , Milkiewicz Piotr , Raszeja-Wyszomirska Joanna , Stachowska Ewa
Published: November 2018

(1) Introduction: Zonulin (ZO) has been proposed as a marker of intestinal permeability. Only a few studies have analyzed to date how diet influences the serum concentration of ZO among patients with non-alcoholic fatty liver disease (NAFLD). We performed a six-month dietetic intervention to evaluate the association between fiber intake and ZO concentration in 32 individuals with NAFLD. (2) Methods: Fiber content in the diet was estimated by Food Frequency Questionnaire (FFQ) and by analyzing 72-h nutritional diaries. ZO concentrations in serum were measured before and after the intervention by immunoenzymatic assay (ELISA). Fatty liver was quantified using the Hamaguchi score before and after the dietetic intervention. (3) Results: During the intervention, the dietary fiber intake increased from 19 g/day to the 29 g/day concomitant with an increase in the frequency of fiber consumption. All patients experienced significant (all p < 0.05) improvements in serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma-glutamyltransferase (GGTP) activities. We also detected decreased serum triglycerides (p = 0.036), homeostatic model assessment insulin resistance (HOMA-IR (p = 0.041) and insulin content (p = 0.34), and improvement of fatty liver status according to the Hamaguchi score (p = 0.009). ZO concentration in serum decreased by nearly 90% (7.335 ± 13.492 vs. 0.507 ± 0.762 ng/mL, p = 0.001) and correlated with the amount of dietary fiber intake (p = 0.043) as well as the degree of fatty liver (p = 0.037). (4) Conclusion: Increasing nutritional fiber results in reduced serum ZO levels, reduced liver enzymes and improved hepatic steatosis in patients with NAFLD, possibly by altering intestinal permeability. Increased dietary fiber intake should be recommended in patients with NAFLD.
The Use of Glycomacropeptide in Patients with Phenylketonuria: A Systematic Review and Meta-Analysis
Authors: Pena Maria João , Pinto Alex , Daly Anne , MacDonald Anita , Azevedo Luís , Rocha Júlio César , Borges Nuno
Published: November 2018

In phenylketonuria (PKU), synthetic protein derived from L-amino acids (AAs) is essential in a low-phenylalanine (Phe) diet. Glycomacropeptide (GMP), an intact protein, is very low in Phe in its native form. It has been modified and adapted for PKU to provide an alternative protein source through supplementation with rate-limiting amino acids (GMP-AAs), although it still contains residual Phe. This review aims to systematically evaluate published intervention studies on the use of GMP-AAs in PKU by considering its impact on blood Phe control (primary aim) and changes in tyrosine control, nutritional biomarkers, and patient acceptability or palatability (secondary aims). Four electronic databases were searched for articles published from 2007 to June 2018. Of the 274 studies identified, only eight were included. Bias risk was assessed and a quality appraisal of the body of evidence was completed. A meta-analysis was performed with two studies with adequate comparable methodology which showed no differences between GMP-AAs and AAs for any of the interventions analysed. This work underlines the scarcity and nature of studies with GMP-AAs interventions. All were short-term with small sample sizes. There is a need for better-designed studies to provide the best evidence-based recommendations.
Vitamin D Status and Seasonal Variation among Danish Children and Adults: A Descriptive Study
Authors: Hansen Louise , Tjønneland Anne , Køster Brian , Brot Christine , Andersen Rikke , Cohen Arieh S. , Frederiksen Kirsten , Olsen Anja
Published: November 2018

The aim of the present study was to describe vitamin D status and seasonal variation in the general Danish population. In this study, 3092 persons aged 2 to 69 years (2565 adults, 527 children) had blood drawn twice (spring and autumn) between 2012 and 2014. A sub-sample of participants had blood samples taken monthly over a year. Serum 25-hydroxyvitamin D (25(OH)D) concentrations were measured by liquid chromatography mass spectrometry, and information on supplement use was assessed from questionnaires. Seasonal variations in 25(OH)D concentrations were evaluated graphically and descriptively, and status according to age, sex, and supplement use was described. It was found that 86% of both adults and children were vitamin D-sufficient in either spring and or/autumn; however, many had a spring concentration below 50 nmol/L. A wide range of 25(OH)D concentrations were found in spring and autumn, with very low and very high values in both seasons. Among adults, women in general had higher median 25(OH)D concentrations than men. Furthermore, vitamin D supplement use was substantial and affected the median concentrations markedly, more so during spring than autumn. Seasonal variation was thus found to be substantial, and bi-seasonal measurements are vital in order to capture the sizable fluctuations in vitamin D status in this Nordic population.
Annurca Apple Polyphenols Protect Murine Hair Follicles from Taxane Induced Dystrophy and Hijacks Polyunsaturated Fatty Acid Metabolism toward β-Oxidation
Authors: Riccio Gennaro , Sommella Eduardo , Badolati Nadia , Salviati Emanuela , Bottone Sara , Campiglia Pietro , Dentice Monica , Tenore Gian Carlo , Stornaiuolo Mariano , Novellino Ettore
Published: November 2018

Chemotherapy-induced alopecia (CIA) is a common side effect of conventional chemotherapy and represents a major problem in clinical oncology. Even months after the end of chemotherapy, many cancer patients complain of hair loss, a condition that is psychologically difficult to manage. CIA disturbs social and sexual interactions and causes anxiety and depression. Synthetic drugs protecting from CIA and endowed with hair growth stimulatory properties are prescribed with caution by oncologists. Hormones, growth factors, morphogens could unwontedly protect tumour cells or induce cancer cell proliferation and are thus considered incompatible with many chemotherapy regimens. Nutraceuticals, on the contrary, have been shown to be safe and effective treatment options for hair loss. We here show that polyphenols from Malus Pumila Miller cv Annurca are endowed with hair growth promoting activity and can be considered a safe alternative to avoid CIA. In vitro, Annurca Apple Polyphenolic Extract (AAE) protects murine Hair Follicles (HF) from taxanes induced dystrophy. Moreover, in virtue of its mechanism of action, AAE is herein proven to be compatible with chemotherapy regimens. AAE forces HFs to produce ATP using mitochondrial β-oxidation, reducing Pentose Phosphate Pathway (PPP) rate and nucleotides production. As consequence, DNA replication and mitosis are not stimulated, while a pool of free amino acids usually involved in catabolic reactions are spared for keratin production. Moreover, measuring the effect exerted on Poly Unsaturated Fatty Acid (PUFA) metabolism, we prove that AAE promotes hair-growth by increasing the intracellular levels of Prostaglandins F2α (PGF2α) and by hijacking PUFA catabolites toward β-oxidation.