bluuebunny/crossref_metadata_2025 huggingface dataset:
Defining Biologically Meaningful Biomes Through Floristic, Functional, and Phylogenetic Data
Authors: Cardoso Domingos , Moonlight Peter W. , Ramos Gustavo , Oatley Graeme , Dudley Christopher , Gagnon Edeline , Queiroz Luciano Paganucci de , Pennington R. Toby , Särkinen Tiina E.
Published: December 2021

While we have largely improved our understanding on what biomes are and their utility in global change ecology, conservation planning, and evolutionary biology is clear, there is no consensus on how biomes should be delimited or mapped. Existing methods emphasize different aspects of biomes, with different strengths and limitations. We introduce a novel approach to biome delimitation and mapping, based upon combining individual regionalizations derived from floristic, functional, and phylogenetic data linked to environmentally trained species distribution models. We define “core Biomes” as areas where independent regionalizations agree and “transition zones” as those whose biome identity is not corroborated by all analyses. We apply this approach to delimiting the neglected Caatinga seasonally dry tropical forest biome in northeast Brazil. We delimit the “core Caatinga” as a smaller and more climatically limited area than previous definitions, and argue it represents a floristically, functionally, and phylogenetically coherent unit within the driest parts of northeast Brazil. “Caatinga transition zones” represent a large and biologically important area, highlighting that ecological and evolutionary processes work across environmental gradients and that biomes are not categorical variables. We discuss the differences among individual regionalizations in an ecological and evolutionary context and the potential limitations and utility of individual and combined biome delimitations. Our integrated ecological and evolutionary definition of the Caatinga and associated transition zones are argued to best describe and map biologically meaningful biomes.
High Blood Flow Into the Femur Indicates Elevated Aerobic Capacity in Synapsids Since the Synapsida-Sauropsida Split
Authors: Knaus Philipp L. , van Heteren Anneke H. , Lungmus Jacqueline K. , Sander P. Martin
Published: December 2021

Varanids are the only non-avian sauropsids that are known to approach the warm-blooded mammals in stamina. Furthermore, a much higher maximum metabolic rate (MMR) gives endotherms (including birds) higher stamina than crocodiles, turtles, and non-varanid lepidosaurs. This has led researchers to hypothesize that mammalian endothermy evolved as a second step after the acquisition of elevated MMR in non-mammalian therapsids from a plesiomorphic state of low metabolic rates. In recent amniotes, MMR correlates with the index of blood flow into the femur (Qi), which is calculated from femoral length and the cross-sectional area of the nutrient foramen. Thus,Qimay serve as an indicator of MMR range in extinct animals. Using theQiproxy and phylogenetic eigenvector maps, here we show that elevated MMRs evolved near the base of Synapsida. Non-mammalian synapsids, including caseids, edaphosaurids, sphenacodontids, dicynodonts, gorgonopsids, and non-mammalian cynodonts, showQivalues in the range of recent endotherms and varanids, suggesting that raised MMRs either evolved in synapsids shortly after the Synapsida-Sauropsida split in the Mississippian or that the low MMR of lepidosaurs and turtles is apomorphic, as has been postulated for crocodiles.
Efficient Evaporative Cooling and Pronounced Heat Tolerance in an Eagle-Owl, a Thick-Knee and a Sandgrouse
Authors: Czenze Zenon J. , Freeman Marc T. , Kemp Ryno , van Jaarsveld Barry , Wolf Blair O. , McKechnie Andrew E.
Published: December 2021

Avian evaporative cooling and the maintenance of body temperature (Tb) below lethal limits during heat exposure has received more attention in small species compared to larger-bodied taxa. Here, we examined thermoregulation at air temperatures (Tair) approaching and exceeding normothermic Tb in three larger birds that use gular flutter, thought to provide the basis for pronounced evaporative cooling capacity and heat tolerance. We quantified Tb, evaporative water loss (EWL) and resting metabolic rate (RMR) in the ∼170-g Namaqua sandgrouse (Pterocles namaqua), ∼430-g spotted thick-knee (Burhinus capensis) and ∼670-g spotted eagle-owl (Bubo africanus), using flow-through respirometry and a stepped Tair profile with very low chamber humidities. All three species tolerated Tair of 56–60°C before the onset of severe hyperthermia, with maximum Tb of 43.2°C, 44.3°C, and 44.2°C in sandgrouse, thick-knees and eagle-owls, respectively. Evaporative scope (i.e., maximum EWL/minimum thermoneutral EWL) was 7.4 in sandgrouse, 12.9 in thick-knees and 7.8 in eagle-owls. The relationship between RMR and Tair varied substantially among species: whereas thick-knees and eagle-owls showed clear upper critical limits of thermoneutrality above which RMR increased rapidly and linearly, sandgrouse did not. Maximum evaporative heat loss/metabolic heat production ranged from 2.8 (eagle-owls) to 5.5 (sandgrouse), the latter the highest avian value yet reported. Our data reveal some larger species with gular flutter possess pronounced evaporative cooling capacity and heat tolerance and, when taken together with published data, show thermoregulatory performance varies widely among species larger than 250 g. Our data for Namaqua sandgrouse reveal unexpectedly pronounced variation in the metabolic costs of evaporative cooling within the genus Pterocles.
Identification of Crucial Genes and Infiltrating Immune Cells Underlying Sepsis-Induced Cardiomyopathy via Weighted Gene Co-Expression Network Analysis
Authors: Li Juexing , Zhou Lei , Li Zhenhua , Yang Shangneng , Tang Liangyue , Gong Hui
Published: December 2021

Sepsis-induced cardiomyopathy (SIC), with a possibly reversible cardiac dysfunction, is a potential complication of septic shock. Despite quite a few mechanisms including the inflammatory mediator, exosomes, and mitochondrial dysfunction, having been confirmed in the existing research studies we still find it obscure about the overall situation of gene co-expression that how they can affect the pathological process of SIC. Thus, we intended to find out the crucial hub genes, biological signaling pathways, and infiltration of immunocytes underlying SIC. It was weighted gene co-expression network analysis that worked as our major method on the ground of the gene expression profiles: hearts of those who died from sepsis were compared to hearts donated by non-failing humans which could not be transplanted for technical reasons (GSE79962). The top 25 percent of variant genes were abstracted to identify 10 co-expression modules. In these modules, brown and green modules showed the strongest negative and positive correlation with SIC, which were primarily enriched in the bioenergy metabolism, immunoreaction, and cell death. Next, nine genes (LRRC39, COQ10A, FSD2, PPP1R3A, TNFRSF11B, IL1RAP, DGKD, POR, and THBS1) including two downregulated and seven upregulated genes which were chosen as hub genes that meant the expressive level of which was higher than the counterparts in control groups. Then, the gene set enrichment analysis (GSEA) demonstrated a close relationship of hub genes to the cardiac metabolism and the necroptosis and apoptosis of cells in SIC. Concerning immune cells infiltration, a higher level of neutrophils and B cells native and a lower level of mast cells resting and plasma cells had been observed in patients with SIC. In general, nine candidate biomarkers were authenticated as a reliable signature for deeper exploration of basic and clinical research studies on SIC.
m6A Modification Patterns With Distinct Immunity, Metabolism, and Stemness Characteristics in Soft Tissue Sarcoma
Authors: Huang Zhen-Dong , Lin Lu-Lu , Liu Zi-Zhen , Hu Chao , Gu Hui-Yun , Wei Ren-Xiong
Published: December 2021

N6-methyladenosine (m6A) RNA methylation has been shown to have prognostic value in cancer. Nonetheless, its potential role regarding immunity, metabolism, and stemness in soft tissue sarcoma (STS) remains unknown. We comprehensively estimated the m6A modification patterns and corresponding immunity, metabolism, and stemness characteristics based on 568 STS samples and 21 m6A regulators. The m6Ascore was constructed to quantify m6A modification patterns in individuals using machine learning algorithms. Two distinct m6A modification patterns among the STS patients were identified, which exhibited differences in prognosis, immune cell infiltration, metabolic pathways, stemness, somatic mutation, and copy number variation. Thereafter, immunity-, metabolism-, and stemness phenotype-related genes associated with m6A modification were identified. Furthermore, patients with lower m6Ascores had increased antitumor immune responses, survival benefit under immunotherapy, tumor mutation burden, immunogenicity, and response to anti-PD-1/L1 immunotherapy. Immunotherapy sensitivity was validated using the IMvigor210 dataset. STS patients with lower m6Ascore might be more sensitive to docetaxel and gemcitabine. Finally, pan-cancer analysis illustrated the significant correlations of m6Ascore with clinical outcomes, immune cell infiltration, metabolism, and stemness. This study revealed that m6A modification plays an important role in immunity, metabolism, and stemness in STS. Evaluating the m6A modification pattern and development of m6Ascore may help to guide more effective immunotherapy and chemotherapy strategies.
Molecular Basis of Selective Cytokine Signaling Inhibition by Antibodies Targeting a Shared Receptor
Authors: Fields James K. , Kihn Kyle , Birkedal Gabriel S. , Klontz Erik H. , Sjöström Kjell , Günther Sebastian , Beadenkopf Robert , Forsberg Göran , Liberg David , Snyder Greg A. , Deredge Daniel , Sundberg Eric J.
Published: December 2021

Interleukin-1 (IL-1) family cytokines are potent mediators of inflammation, acting to coordinate local and systemic immune responses to a wide range of stimuli. Aberrant signaling by IL-1 family cytokine members, however, is linked to myriad inflammatory syndromes, autoimmune conditions and cancers. As such, blocking the inflammatory signals inherent to IL-1 family signaling is an established and expanding therapeutic strategy. While several FDA-approved IL-1 inhibitors exist, including an Fc fusion protein, a neutralizing antibody, and an antagonist cytokine, none specifically targets the co-receptor IL-1 receptor accessory protein (IL-1RAcP). Most IL-1 family cytokines form productive signaling complexes by binding first to their cognate receptors – IL-1RI for IL-1α and IL-1β; ST2 for IL-33; and IL-36R for IL-36α, IL-36β and IL-36γ – after which they recruit the shared secondary receptor IL-1RAcP to form a ternary cytokine/receptor/co-receptor complex. Recently, IL-1RAcP was identified as a biomarker for both AML and CML. IL-1RAcP has also been implicated in tumor progression in solid tumors and an anti-IL1RAP antibody (nadunolimab, CAN04) is in phase II clinical studies in pancreatic cancer and non-small cell lung cancer (NCT03267316). As IL-1RAcP is common to all of the abovementioned IL-1 family cytokines, targeting this co-receptor raises the possibility of selective signaling inhibition for different IL-1 family cytokines. Indeed, previous studies of IL-1β and IL-33 signaling complexes have revealed that these cytokines employ distinct mechanisms of IL-1RAcP recruitment even though their overall cytokine/receptor/co-receptor complexes are structurally similar. Here, using functional, biophysical, and structural analyses, we show that antibodies specific for IL-1RAcP can differentially block signaling by IL-1 family cytokines depending on the distinct IL-1RAcP epitopes that they engage. Our results indicate that targeting a shared cytokine receptor is a viable therapeutic strategy for selective cytokine signaling inhibition.
Apoptosis, Autophagy, NETosis, Necroptosis, and Pyroptosis Mediated Programmed Cell Death as Targets for Innovative Therapy in Rheumatoid Arthritis
Authors: Zhao Jianan , Jiang Ping , Guo Shicheng , Schrodi Steven J. , He Dongyi
Published: December 2021

Rheumatoid arthritis (RA) is a chronic inflammatory joint disease that can lead to clinical manifestations of systemic diseases. Its leading features include chronic synovial inflammation and degeneration of the bones and joints. In the past decades, multiple susceptibilities for rheumatoid arthritis have been identified along with the development of a remarkable variety of drugs for its treatment; which include analgesics, glucocorticoids, nonsteroidal anti-inflammatory medications (NSAIDs), disease-modifying anti-rheumatic drugs (DMARDs), and biologic response modifiers (bDMARDs). Despite the existence of many clinical treatment options, the prognosis of some patients remains poor due to complex mechanism of the disease. Programmed cell death (PCD) has been extensively studied and ascertained to be one of the essential pathological mechanisms of RA. Its dysregulation in various associated cell types contributes to the development of RA. In this review, we summarize the role of apoptosis, cell death-associated neutrophil extracellular trap formation, necroptosis, pyroptosis, and autophagy in the pathophysiology of RA to provide a theoretical reference and insightful direction to the discovery and development of novel therapeutic targets for RA.
Stopping Macroplastic and Microplastic Pollution at Source by Installing Novel Technologies in River Estuaries and Waste Water Treatment Plants: The CLAIM Project
Authors: Gkanasos Athanasios , Tsiaras Kostas , Triantaphyllidis George , Panagopoulos Aleksandros , Pantazakos George , Owens Tristan , Karametsis Christos , Pollani Annika , Nikoli Elisabeth , Katsafados Nikolaos , Triantafyllou George
Published: December 2021

Marine pollution from debris is a major issue nowadays, since every year large amounts of litter enter into the sea. Under the Horizon 2020 framework and within the Cleaning Litter by developing and Applying Innovative Methods in European Seas (CLAIM) project, innovative devices were designed, developed, tested and applied in laboratory and in the field. These consisted of a system named CLEAN TRASH for the prevention of macrolitter in river estuaries before entering the Sea and a filtering system for microplastics (MPs), to be placed at waste water treatment plants (WWTP). Laboratory experiments showed that macrolitters were blocked by 90% by the CLEAN TRASH system, while during the sea testing period at the Kifissos river estuary, a significant source of terrestrial based litter for the Saronikos Gulf, a total amount of 1,175 kg of litter was collected in 38 days before entering the sea, of which the 708 kg (60%) were plastic debris of various sizes and another 164 kg (14%) of styrofoam parts. The lab scale prototype of the filtering system for MPs had an efficiency of about 95%. The upscaled device was tested at the Megara WWTP and was able to withhold a significant amount of MPs. The theoretical contribution of such devices toward the reduction of plastic pollution in the Saronikos Gulf area and the Natura conservation areas therein, was also studied with the use of a 3-D coupled Hydrodynamic-Lagrangian litter tracking model. In all experiments performed, the installation of the above devices for a period of 2 years, resulted in a microplastics reduction by about 87% and a macroplastics reduction ranging from 13 to 43%, depending on the sources.
Nearshore Dissolved and Particulate Organic Matter Dynamics in the Southwestern Baltic Sea: Environmental Drivers and Time Series Analysis (2010–2020)
Authors: Osterholz Helena , Burmeister Christian , Busch Susanne , Dierken Madleen , Frazão Helena C. , Hansen Regina , Jeschek Jenny , Kremp Anke , Kreuzer Lars , Sadkowiak Birgit , Waniek Joanna J. , Schulz-Bull Detlef E.
Published: December 2021

Dissolved and particulate organic carbon (DOC, POC) and nitrogen (DON, PON) constitute essential nutrient and energy sources to heterotrophic microbes in aquatic systems. Especially in the shallow coastal ocean, the concentrations are highly variable on short timescales, and cycling is heavily affected by different sources and environmental drivers. We analyzed surface water organic carbon and nitrogen concentrations determined weekly from 2010 to 2020 in the nearshore southwestern Baltic Sea (Heiligendamm, Germany) in relation to physical, chemical and biological parameters available since 1988. Mixing of low-DOC North Sea water with high-DOC Baltic Sea water, as well as in situ primary production, were confirmed as the main drivers of organic carbon and nitrogen concentrations. Tight coupling between POC, PON, chlorophyll a and phytoplankton carbon with DON seasonal dynamics corroborated the close relationship between phytoplankton production and degradation of organic nutrients with preferential remineralization of nitrogen. Significant changes in air and water temperature, salinity, and inorganic nutrients over time indicated effects of climate change and improved water quality management in the eutrophic Baltic Sea. Bulk organic nutrient concentrations did not change over time, while the salinity-corrected fraction of the DOC increased by about 0.6 μmol L–1yr–1. Concurrently, chlorophyll a and Bacillariophyceae and Cryptophyceae carbon increased, denoting a potential link to primary productivity. The high variability of the shallow system exacerbates the detection of trends, but our results emphasize the value of these extended samplings to understand coupled biogeochemical cycling of organic matter fractions and to detect trends in these important carbon reservoirs.
Evaluating Strategies for Managing Anthropogenic Mortality on Marine Mammals: An R Implementation With the Package RLA
Authors: Genu Mathieu , Gilles Anita , Hammond Philip S. , Macleod Kelly , Paillé Jade , Paradinas Iosu , Smout Sophie , Winship Arliss J. , Authier Matthieu
Published: December 2021

Bycatch, the undesirable and non-intentional catch of non-target species in marine fisheries, is one of the main causes of mortality of marine mammals worldwide. When quantitative conservation objectives and management goals are clearly defined, computer-based procedures can be used to explore likely population dynamics under different management scenarios and estimate the levels of anthropogenic removals, including bycatch, that marine mammal populations may withstand. Two control rules for setting removal limits are the Potential Biological Removal (PBR) established under the US Marine Mammal Protection Act and the Removals Limit Algorithm (RLA) inspired from the Catch Limit Algorithm (CLA) developed under the Revised Management Procedure of the International Whaling Commission. The PBR and RLA control rules were tested in a Management Strategy Evaluation (MSE) framework. A key feature of PBR and RLA is to ensure conservation objectives are met in the face of the multiple uncertainties or biases that plague real-world data on marine mammals. We built a package named RLA in the R software to carry out MSE of control rules to set removal limits in marine mammal conservation. The package functionalities are illustrated by two case studies carried out under the auspices of the Oslo and Paris convention (OSPAR) (the Convention for the Protection of the Marine Environment of the North-East Atlantic) Marine Mammal Expert Group (OMMEG) in the context of the EU Marine Strategy Framework Directive. The first case study sought to tune the PBR control rule to the conservation objective of restoring, with a probability of 0.8, a cetacean population to 80% of carrying capacity after 100 years. The second case study sought to further develop a RLA to set removals limit on harbor porpoises in the North Sea with the same conservation objective as in the first case study. Estimation of the removals limit under the RLA control rule was carried out within the Bayesian paradigm. Outputs from the functions implemented in the package RLA allows the assessment of user-defined performance metrics, such as time to reach a given fraction of carrying capacity under a given level of removals compared to the time needed given no removals.
A Regime Shift Toward a More Anoxic Environment in a Eutrophic Sea in Northern Europe
Authors: Almroth-Rosell Elin , Wåhlström Iréne , Hansson Martin , Väli Germo , Eilola Kari , Andersson Pia , Viktorsson Lena , Hieronymus Magnus , Arneborg Lars
Published: December 2021

Dissolved oxygen in the sea is essential for marine fauna and biogeochemical processes. Decline in the sea water oxygen concentration is considered to be an effect of eutrophication, also exacerbated by climate change. The Baltic Sea is one of the most eutrophic seas in the world and is located in northern Europe. It is a vulnerable, brackish, semi-enclosed sea, suffering from high pressures from human activity. This leads to increased hypoxic and anoxic areas, which can be used as a measure of the environmental state. In the present study the extent of anoxic (O2 < 0 ml l–1) and hypoxic (O2 < 2 ml l–1) areas were estimated for the autumns in 1960–2019 using vertical profiles of observed oxygen concentrations in the Baltic proper and four sub-areas of the Baltic proper: the Bornholm Basin, the western, northern and eastern Gotland basins. From vertical profiles of observed salinity, the annual average of the halocline depths in the four sub-basins were estimated. The results imply regime shifts toward increased anoxic area extents in the Gotland basins around the turn of the 20th century. In autumn 2018, the extent of anoxic bottom areas in the Baltic Sea was record high since the start of the data series. During the later part of the studied period the depths of the halocline coincide with the depth of the hypoxia in the Gotland basins. This implies that in these basins a worst-case scenario for the extent of hypoxic areas seems to be reached.
The 2019 Benguela Niño
Authors: Imbol Koungue Rodrigue Anicet , Brandt Peter , Lübbecke Joke , Prigent Arthur , Martins Meike Sena , Rodrigues Regina R.
Published: December 2021

High interannual sea surface temperature anomalies of more than 2°C were recorded along the coasts of Angola and Namibia between October 2019 and January 2020. This extreme coastal warm event that has been classified as a Benguela Niño, reached its peak amplitude in November 2019 in the Angola Benguela front region. In contrast to classical Benguela Niños, the 2019 Benguela Niño was generated by a combination of local and remote forcing. In September 2019, a local warming was triggered by positive anomalies of near coastal wind-stress curl leading to downwelling anomalies through Ekman dynamics off Southern Angola and by anomalously weak winds reducing the latent heat loss by the ocean south of 15°S. In addition, downwelling coastal trapped waves were observed along the African coast between mid-October 2019 and early January 2020. Those coastal trapped waves might have partly emanated from the equatorial Atlantic as westerly wind anomalies were observed in the central and eastern equatorial Atlantic between end of September to early December 2019. Additional forcing for the downwelling coastal trapped waves likely resulted from an observed weakening of the prevailing coastal southerly winds along the Angolan coast north of 15°S between October 2019 and mid-February 2020. During the peak of the event, latent heat flux damped the sea surface temperature anomalies mostly in the Angola Benguela front region. In the eastern equatorial Atlantic, relaxation of cross-equatorial southerly winds might have contributed to the equatorial warming in November 2019 during the peak of the 2019 Benguela Niño. Moreover, for the first time, moored velocities off Angola (11°S) revealed a coherent poleward flow in the upper 100 m in October and November 2019 suggesting a contribution of meridional heat advection to the near-surface warming during the early stages of the Benguela Niño. During the Benguela Niño, a reduction of net primary production in the Southern Angola and Angola Benguela front regions was observed.
Effects of Microalgal Food Quantity on Several Productivity-Related Parameters of the Calanoid Copepod Bestiolina similis (Calanoida: Paracalanidae)
Authors: Camus Thomas , Rolla Lucrezia , Jiang Jufeng , Zeng Chaoshu
Published: December 2021

The optimization of copepod feeding protocol is paramount to improve culture productivity and to maintain favorable water quality parameters overtime, as well as saving operational costs by preventing the production of unnecessary quantities of microalgae. The influence of microalgal feeding concentration on major parameters related to culture productivity of the calanoid copepod Bestiolina similis (Paracalanidae) was investigated in a series of laboratory experiments. B. similis was fed eight different concentrations (0, 150, 300, 600, 900, 1,200, 1,500 and 1,800 μgC l–1) of a mixed microalgal diet consisting of Tahitian strain of Isochrysis species, Pavalova 50 and Tetraselmis chuii at 1:1:1 carbon ratio. The results indicate that female daily and cumulative egg production over lifespan, egg hatching rate, naupliar and copepodite survival and development, adult female life expectancy, population growth and fecal pellet production rate (FPPR) were all significantly affected by microalgae feeding ration. Conversely, no significant influence could be established between microalgae food concentration and egg diameter or adult sex ratio. Feeding rations as low as 150 μgC l–1 led to lower egg hatching rates, survival and development, adult female life expectancy and population growth compared with the higher microalgae rations tested. Feeding concentration ≤ 900 μgC l–1 significantly limited female daily egg and fecal pellet production rate, as well as their cumulative egg production over lifespan, when compared to a level of 900 μgC l–1. Bestiolina similis fed with 1,200 μgC l–1 significantly improved female egg and fecal pellet production when compared to the lower treatments and was responsible for the highest female lifespan egg production and population growth observed among all treatments. Feeding rations as high as 1,500 μgC l–1 and 1,800 μgC l–1 did not lead to significant improvement in any of the parameters measured. This is likely due to a saturation effect at high food concentration which is known to decrease calanoid copepods feeding efficiency. Finally, B. similis FPPR, used as a proxy for ingestion, was found to saturate at a microalgae concentration of 783.4 μgC l–1 using a non-linear Michael-Menton (2 parameters), indicating that CVI female ingestion did not increase significantly above this concentration. Based on the above results it is recommended that B. similis cultures should be fed at a concentration of 1,200 μgC l–1, and not above, as rations > 1,200 μgC l–1 will not significantly improve any of the productivity-related parameters observed in this study. Feeding rations should never be below 783.40 μgC l–1 as this is the threshold level below which adult female ingestion rates become limiting.
Ultrathin Spin-Decoupled Meta-Devices for Independent Control of Electromagnetic Waves With Dual-Orthogonal Circular Polarization
Authors: Chen Wenqiong , Qi Buxiong , Zhang Jingwei , Niu Tiaoming , Mei Zhonglei
Published: December 2021

Inspired by the concept of miniaturized and integrated systems, an ultrathin and multifunctional metasurface device is highly desirable in microwave fields. It is an inherent characteristic that the two spin phase states of electromagnetic waves imparted by the geometric phase are always conjugate symmetric, i.e., the geometric phase produces anti-symmetrical phase responses between dual-orthogonal circular polarization states. So it is extremely crucial to break the conjugate constraints and realize the completely independent control of electromagnetic waves with dual-orthogonal circular polarization. Based on this perspective, ultrathin and bifunctional meta-devices operating in reflection mode are proposed to independently manipulate the left-handed and right-handed circularly polarized waves, which are constructed by anisotropic meta-atoms with synthetical geometric and propagation phases. It is worth noting that the component elements only need a single-layer structure with the thickness of 0.07λ0. Several design samples are presented to achieve functionalities of beam focusing, vortex wave generation, and beam deflection, respectively. Experiments are performed and show good consistence with the simulation results, successfully verifying the performance of the designed metasurfaces. The research results in this paper pave the way to design low-profile and bifunctional devices with independent controls of circularly polarized waves, which is expected to expand the working capacity of metasurfaces to realize complex electromagnetic wave manipulation with a new degree of freedom.
Developing a Screening Procedure During the COVID-19 Pandemic: Process and Challenges Faced by a Low-Incidence Area
Authors: Tang Wei , Wang Fei , Wang Jian-Wei , Huang Yao , Liu Li , Zhao Shi-Jun , Zhao Xin-Ming , Wu Ning
Published: December 2021

Purpose: To summarize the imaging results of COVID-19 pneumonia and develop a computerized tomography (CT) screening procedure for patients at our institution with malignant tumors.

Methods: Following epidemiological investigation, 1,429 patients preparing to undergo anti-tumor-treatment underwent CT scans between February 17 and April 16, 2020. When CT findings showed suspected COVID-19 pneumonia after the supervisor radiologist and the thoracic experience radiologist had double-read the initial CT images, radiologists would report the result to our hospital infection control staff. Further necessary examinations, including the RT-PCR test, in the assigned hospital was strongly recommended for patients with positive CT results. The CT examination room would perform sterilization for 30 min to 1 h. If the negative results of any suspected COVID-19 pneumonia CT findings were identified, the radiologists would upload the results to our Hospital Information Systems and inform clinicians within 2 h.

Results: Fifty (0.35%, 50/1,429) suspected pneumonia cases, including 29 males and 21 females (median age: 59.5 years old; age range 27–79 years), were identified. A total of 34.0% (17/50) of the patients had a history of lung cancer and 54.0 (27/50) underwent chemotherapy or targeted therapy. Forty-six patients (92.0%) had prior CT scans, and 35 patients (76.1%) with suspected pneumonia were newly seen (median interval time: 62 days). Sub-pleura small patchy or strip-like lesions most likely due to fibrosis or hypostatic pneumonia and cluster of nodular lesions were the two main signs of suspected cases on CT images (34, 68.0%). Twenty-seven patients (54.0%) had, at least once, follow-up CT scan (median interval time: 18.0 days). Only one patient had an increase in size (interval time: 8 days), the immediately RT-PCR test result was negative.

Conclusion: CT may be useful as a screening tool for COVID-19 based on imaging features. But the differential diagnosis between COVID-19 and other pulmonary infection and/or non-infectious disease is very difficult due to its overlapping imaging features.The confirmed diagnosis of the COVID-19 infection should be based on the etiologic eventually. The cancer patients at a low-incidence area would continue treatment by screening carefully before admission.
Continuous High-Dose (6 mg) vs. Low-Dose (3 mg) Intravitreal Ganciclovir for Cytomegalovirus Retinitis After Haploidentical Hematopoietic Stem Cell Transplantation: A Randomized Controlled Study
Authors: Chen Wei-Bin , Long Ze , Hou Jing , Miao Heng , Zhao Ming-wei
Published: December 2021

Purpose: To evaluate the safety and efficacy of continuous high-dose (6 mg) intravitreal ganciclovir injections (IVG) for cytomegalovirus (CMV) retinitis (CMVR) after haploidentical hematopoietic stem cell transplantation (Haplo-HSCT), and to explore factors that may influence the treatment procedure.

Design: Prospective, randomized, single-blinded, positive-controlled, interventional, comparative study.

Methods: A total of 22 patients with CMVR (32 eyes) were randomized to either high-dose group (IVG 6 mg weekly) or low-dose group (IVG 3 mg given twice weekly for 2 weeks as induction phase and weekly thereafter as maintenance phase). Patients who were recorded any positive CMV DNAemia or other active CMV diseases and needed systemic anti-CMV treatment during the study period were excluded. The vision outcome, variables of the treatment procedure, and incidence of complication and CMVR recurrence were analyzed and compared. Logistic regression was applied to determine the factors that may have an impact on the treatment process at baseline.

Results: Compared to the low-dose group, the high-dose group resulted in a median of two less intravitreal injections (4 vs. 6 times, respectively, P = 0.016), while the rate of vision stability or improvement (81.2 vs. 87.5%), the incidence of complication (6.2 vs. 18.8%), and CMVR recurrence (12.5% vs. 6.2%) were similar (all P > 0.05). No drug-related toxicity was observed. Initial aqueous CMV-DNA load (OR: 6.872, 95% CI: 1.335–35.377, P = 0.021) and extension of lesion (OR: 0.942, 95% CI: 0.897 to .991, P = 0.020), but not dosing regimen (P = 0.162), were predictors of the treatment duration.

Conclusions: Continuous high-dose regimen was well tolerated and resulted in less intravitreal injections, with similar vision outcomes and safety profiles. The clinical course of CMVR after Haplo-HSCT was determined by its own nature at baseline and could not be modified by treatment protocols under consistent immune background.
Circulating Placental Alkaline Phosphatase Expressing Exosomes in Maternal Blood Showed Temporal Regulation of Placental Genes
Authors: Parveen Arshiya , Mishra Suman , Srivastava Medha , Chaudhary Dharmendra K. , Kapoor Deepa , Gupta Amrit , Tiwari Swasti
Published: December 2021

Background:Analysis of placental genes could unravel maternal-fetal complications. However, inaccessibility to placental tissue during early pregnancy has limited this effort. We tested if exosomes (Exo) released by human placenta in the maternal circulation harbor crucial placental genes.

Methods:Placental alkaline phosphate positive exosomes (ExoPLAP) were enriched from maternal blood collected at the following gestational weeks; 6–8th (T1), 12–14th (T2), 20–24th (T3), and 28th−32nd (T4). Nanotracking analysis, electron microscopy, dynamic light scattering, and immunoblotting were used for characterization. We used microarray for transcriptome and quantitative PCR (qPCR) for gene analysis in ExoPLAP.

Results:Physical characterization and presence of CD63 and CD9 proteins confirmed the successful ExoPLAP enrichment. Four of the selected 36 placental genes did not amplify in ExoPLAP, while 32 showed regulations (n= 3–8/time point). Most genes in ExoPLAP showed significantly lower expression at T2–T4, relative to T1 (p< 0.05), such asNOS3, TNFSF10, OR5H6, APOL3, andNEDD4L. In contrast, genes, such asATF6, NEDD1, andIGF2, had significantly higher expression at T2–T4 relative to T1. Unbiased gene profiling by microarray also confirmed expression of above genes in ExoPLAP-transcriptome. In addition, repeated measure ANOVA showed a significant change in the ExoPLAP transcriptome from T2 to T4 (n= 5/time point).

Conclusion:Placental alkaline phosphate positive exosomes transcriptome changed with gestational age advancement in healthy women. The transcriptome expressed crucial placental genes involved in early embryonic development, such as actin cytoskeleton organization, appropriate cell positioning, DNA replication, and B-cell regulation for protecting mammalian fetuses from rejection. Thus, ExoPLAP in maternal blood could be a promising source to study the placental genes regulation for non-invasive monitoring of placental health.
Red Blood Cell Extracellular Vesicle-Based Drug Delivery: Challenges and Opportunities
Authors: Chiangjong Wararat , Netsirisawan Pukkavadee , Hongeng Suradej , Chutipongtanate Somchai
Published: December 2021

Recently, red blood cell-derived extracellular vesicles (RBCEVs) have attracted attention for clinical applications because of their safety and biocompatibility. RBCEVs can escape macrophages through the binding of CD47 to inhibitory receptor signal regulatory protein α. Furthermore, genetic materials such as siRNA, miRNA, mRNA, or single-stranded RNA can be encapsulated within RBCEVs and then released into target cells for precise treatment. However, their side effects, half-lives, target cell specificity, and limited large-scale production under good manufacturing practice remain challenging. In this review, we summarized the biogenesis and composition of RBCEVs, discussed the advantages and disadvantages of RBCEVs for drug delivery compared with synthetic nanovesicles and non-red blood cell-derived EVs, and provided perspectives for overcoming current limitations to the use of RBCEVs for clinical applications.
The Association of Sleep Duration With Vision Impairment in Middle-Aged and Elderly Adults: Evidence From the China Health and Retirement Longitudinal Study
Authors: Sun Mengsha , Bo Qiyu , Lu Bing , Sun Xiaodong , Zhou Minwen
Published: December 2021

Objective: This study aims to investigate the association of sleep duration with vision impairment (VI) in middle-aged and elderly adults.

Methods: This cross-sectional study used the data from the baseline survey of the China Health and Retirement Longitudinal Study (CHARLS) 2011–2012, a national survey of adults aged 45 years or older. Weighted multilevel logistic regression models were used to evaluate the association between self-reported sleep duration and VI.

Results: Of the 13,959 survey respondents, a total of 4,776 (34.2%) reported VI. The prevalence of short (≤6 h/night) and long (>8 h/night) sleep durations was higher among respondents with VI than those without VI (P < 0.001). Multilevel logistic regression models showed that compared with a sleep duration of 6–8 h/night, a sleep duration of ≤6 h/night was associated with a 1.45-fold [95% confidence interval (CI) = 1.34–1.56] higher VI risk, and a sleep duration of >8 h/night was associated with a 1.18-fold (95% CI = 1.03–1.34) higher VI risk, after adjusting for sociodemographic data, lifestyle factors, and health conditions. Vision impairment was associated with short sleep duration in respondents from all age or gender categories. However, VI was associated with long sleep duration in respondents from the elderly or female categories. The association between VI and long sleep duration disappeared in respondents of middle-aged or male categories.

Conclusions: The potential impact of sleep on the risk of visual functions requires further attention. A more comprehensive and integrated health care and rehabilitation system covering vision and sleep is also needed.
Vitrectomy With Peeling the Internal Limiting Membrane for the Treatment of Macular Hole Following Ruptured Retinal Arterial Macroaneurysm: A Case Report
Authors: Liu Zhigao , Wang Shuya , Wang Yu , Ma Aihua , Zhao Bojun
Published: December 2021

Background: This study aimed to report a case of vitrectomy with peeling the internal limiting membrane for the treatment of macular hole (MH) following ruptured retinal arterial macroaneurysm (RAMA).

Case Presentation: A 65-year-old woman noticed a sudden decrease in vision in the left eye. She had no other ocular problems apart from a mild cataract in both eyes before. Her best-corrected visual acuity (BCVA) was 20/33 in the right eye, and 6/100 in the left eye. Fluorescein angiography (FFA) showed a retinal arterial macroaneurysm with telangiectatic retinal vascular changes in the inferior temporal macular region. Optical coherence tomography (OCT) examination demonstrated the presence of subretinal hemorrhage extending into the foveal area and incomplete posterior vitreous detachment. Because of the presence of submacular hemorrhage, some medicine was administrated and the patient was followed up. Then, 5 months later, the hemorrhage was absorbed. OCT examination exhibited a full-thickness MH with a macular epiretinal membrane. The size of the MH was 722 μm in diameter. She was then given a standard three-port pars plana vitrectomy (PPV), along with peeling of the internal limiting membrane (ILM) and filling the vitreous cavity with air. Anatomic closure of the MH was achieved after 4 weeks of the surgery by the examination of OCT. The BCVA was improved to 15/100.

Conclusions: This case expanded our knowledge of the association of MH secondary to ruptured RAMA. We reported a case with successful surgical closure of the MH and improvement of BCVA.
Machine Learning Model for Predicting Acute Respiratory Failure in Individuals With Moderate-to-Severe Traumatic Brain Injury
Authors: Ma Rui Na , He Yi Xuan , Bai Fu Ping , Song Zhi Peng , Chen Ming Sheng , Li Min
Published: December 2021

Background: There is a high incidence of acute respiratory failure (ARF) in moderate or severe traumatic brain injury (M-STBI), worsening outcomes. This study aimed to design a predictive model for ARF.

Methods: Adult patients with M-STBI [3 ≤ Glasgow Coma Scale (GCS) ≤ 12] with a definite history of brain trauma and abnormal head on CT images, obtained from September 2015 to May 2017, were included. Patients with age >80 years or <18 years, multiple injuries with TBI upon admission, or pregnancy (in women) were excluded. Two models based on machine learning extreme gradient boosting (XGBoost) or logistic regression, respectively, were developed for predicting ARF within 48 h upon admission. These models were evaluated by out-of-sample validation. The samples were assigned to the training and test sets at a ratio of 3:1.

Results: In total, 312 patients were analyzed including 132 (42.3%) patients who had ARF. The GCS and the Marshall CT score, procalcitonin (PCT), and C-reactive protein (CRP) on admission significantly predicted ARF. The novel machine learning XGBoost model was superior to logistic regression model in predicting ARF [area under the receiver operating characteristic (AUROC) = 0.903, 95% CI, 0.834–0.966 vs. AUROC = 0.798, 95% CI, 0.697–0.899; p < 0.05].

Conclusion: The XGBoost model could better predict ARF in comparison with logistic regression-based model. Therefore, machine learning methods could help to develop and validate novel predictive models.
Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host
Authors: Oliveira Fernando , Lima Tânia , Correia Alexandra , Silva Ana Margarida , Soares Cristina , Morais Simone , Weißelberg Samira , Vilanova Manuel , Rohde Holger , Cerca Nuno
Published: December 2021

Iron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there is currently no comprehensive study addressing siderophore production in Staphylococcus epidermidis. Staphylococcus epidermidis is an innocuous skin commensal bacterium. The species, though, has emerged as a leading cause of implant-associated infections, significantly supported by an inherent ability to form biofilms. The process of adaptation from skin niche environments to the hostile conditions during invasion is yet not fully understood. Herein, we addressed the possible role of siderophore production in S. epidermidis virulence. We first identified and deleted a siderophore homolog locus, sfaABCD, and provided evidence for its involvement in iron acquisition. Our findings further suggested the involvement of siderophores in the protection against oxidative stress-induced damage and demonstrated the in vivo relevance of a siderophore-mediated iron acquisition during S. epidermidis infections. Conclusively, this study addressed, for the first time in this species, the underlying mechanisms of siderophore production, highlighting the importance of a siderophore-mediated iron acquisition under host relevant conditions and, most importantly, its contribution to survival within the host.
Ex-vivo Kidney Machine Perfusion: Therapeutic Potential
Authors: Zulpaite Ruta , Miknevicius Povilas , Leber Bettina , Strupas Kestutis , Stiegler Philipp , Schemmer Peter
Published: December 2021

Kidney transplantation remains the gold standard treatment for patients suffering from end-stage kidney disease. To meet the constantly growing organ demands grafts donated after circulatory death (DCD) or retrieved from extended criteria donors (ECD) are increasingly utilized. Not surprisingly, usage of those organs is challenging due to their susceptibility to ischemia-reperfusion injury, high immunogenicity, and demanding immune regulation after implantation. Lately, a lot of effort has been put into improvement of kidney preservation strategies. After demonstrating a definite advantage over static cold storage in reduction of delayed graft function rates in randomized-controlled clinical trials, hypothermic machine perfusion has already found its place in clinical practice of kidney transplantation. Nevertheless, an active investigation of perfusion variables, such as temperature (normothermic or subnormothermic), oxygen supply and perfusate composition, is already bringing evidence that ex-vivo machine perfusion has a potential not only to maintain kidney viability, but also serve as a platform for organ conditioning, targeted treatment and even improve its quality. Many different therapies, including pharmacological agents, gene therapy, mesenchymal stromal cells, or nanoparticles (NPs), have been successfully delivered directly to the kidney during ex-vivo machine perfusion in experimental models, making a big step toward achievement of two main goals in transplant surgery: minimization of graft ischemia-reperfusion injury and reduction of immunogenicity (or even reaching tolerance). In this comprehensive review current state of evidence regarding ex-vivo kidney machine perfusion and its capacity in kidney graft treatment is presented. Moreover, challenges in application of these novel techniques in clinical practice are discussed.
Pretreatment Affects Profits From Xylanase During Enzymatic Saccharification of Corn Stover Through Changing the Interaction Between Lignin and Xylanase Protein
Authors: Feng Xiaoting , Yao Yini , Xu Nuo , Jia Hexue , Li Xuezhi , Zhao Jian , Chen Shicheng , Qu Yinbo
Published: December 2021

Effective pretreatment is vital to improve the biomass conversion efficiency, which often requires the addition of xylanase as an accessory enzyme to enhance enzymatic saccharification of corn stover. In this study, we investigated the effect of two sophisticated pretreatment methods including ammonium sulfite (AS) and steam explosion (SE) on the xylanase profits involved in enzymatic hydrolysis of corn stover. We further explored the interactions between lignin and xylanase Xyn10A protein. Our results showed that the conversion rates of glucan and xylan in corn stover by AS pretreatment were higher by Xyn10A supplementation than that by SE pretreatment. Compared with the lignin from SE pretreated corn stover, the lignin from AS pretreated corn stover had a lower Xyn10A initial adsorption velocity (13.56 vs. 10.89 mg g−1 min−1) and adsorption capacity (49.46 vs. 27.42 mg g−1 of lignin) and weakened binding strength (310.6 vs. 215.9 L g−1). Our study demonstrated the low absolute zeta potential and strong hydrophilicity of the lignin may partly account for relative weak interaction between xylanase protein and lignin from AS pretreated corn stover. In conclusion, our results suggested that AS pretreatment weakened the inhibition of lignin to enzyme, promoted the enzymatic hydrolysis of corn stover, and decreased the cost of enzyme in bioconversion.