bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Microbiota in Infant Health: From Early Life to Adulthood
Authors: Yao Yao , Cai Xiaoyu , Ye Yiqing , Wang Fengmei , Chen Fengying , Zheng Caihong
Published: October 2021

From early life to adulthood, the microbiota play a crucial role in the health of the infant. The microbiota in early life are not only a key regulator of infant health but also associated with long-term health. Pregnancy to early life is the golden time for the establishment of the infant microbiota, which is affected by both environmental and genetic factors. Recently, there is an explosion of the studies on the role of microbiota in human diseases, but the application to disease or health is relatively limited because many aspects of human microbiota remain controversial, especially about the infant microbiota. Therefore, a critical and conclusive review is necessary to understand fully the relationship between the microbiota and the health of infant. In this article, we introduce in detail the role of microbiota in the infant from pregnancy to early life to long-term health. The main contents of this article include the relationship between the maternal microbiota and adverse pregnancy outcomes, the establishment of the neonatal microbiota during perinatal period and early life, the composition of the infant gut microbiota, the prediction of the microbiota for long-term health, and the future study directions of microbiota.
NK Cell Reconstitution After Autologous Hematopoietic Stem Cell Transplantation: Association Between NK Cell Maturation Stage and Outcome in Multiple Myeloma
Authors: Orrantia Ane , Terrén Iñigo , Astarloa-Pando Gabirel , González Carmen , Uranga Alasne , Mateos-Mazón Juan J. , García-Ruiz Juan C. , Riñón Marta , Rey Mercedes , Pérez-Fernandez Silvia , Zenarruzabeitia Olatz , Borrego Francisco
Published: October 2021

Autologous hematopoietic stem cell transplantation (autoHSCT) is a standard of care for transplant-eligible patients with multiple myeloma (MM). Among factors that influence outcome after autoHSCT, it has been suggested that the number of natural killer (NK) cells plays an important role. However, the impact that different NK cell subsets and their phenotype could have in disease progression after autoHSCT are less clear. For this reason, we have phenotypically and functionally characterized NK cells during immune system reconstitution after autoHSCT in 54 MM patients. Shortly after leukocyte recovery, an extensive redistribution of NK cell subsets occurs in these patients. In addition, NK cells undergo a profound phenotypic change characterized, among others, by their increased proliferative capacity and immature phenotype. Importantly, MM patients who showed lower frequencies of the mature highly differentiated NKG2A-CD57+ NK cell subset at +30 and +100 days after autoHSCT experienced superior progression-free survival and had a longer time to the next treatment than those with higher frequencies. Our results provide significant insights into NK cell reconstitution after autoHSCT and suggest that the degree of NK cell maturation after autoHSCT affects the clinical outcome of MM patients treated with this therapeutic strategy.
Utilization Trends of Antiviral and Antifungal Agents for Human Systemic Use in Tanzania From 2010 to 2017 Using the World Health Organization Collaborating Centre for Drug Statistics Methodology
Authors: Hamim Hafidh , Sangeda Raphael Z. , Bundala Maganga , Mkumbwa Sonia , Bitegeko Adonis , Sillo Hiiti B. , Fimbo Adam M. , Chambuso Mhina , Mbugi Erasto V.
Published: October 2021

Introduction

The increase in antimicrobial consumption contributes to the emergence of antimicrobial resistance (AMR). Many studies have investigated the patterns of antibacterial consumption and antibacterial resistance. However, there is a paucity of data on the utilization of antivirals and antifungals in low and middle-income countries to serve as a baseline for monitoring and surveillance of AMR. Therefore, this study determined Tanzania’s systemic antifungal and antiviral utilization trends from 2010 to 2017, based on the Tanzania Medicines and Medical Devices Authority (TMDA) medicine importation archives.

Methodology

An analytical, longitudinal retrospective survey covering 2010 to 2017 was conducted. The study utilized the Anatomical Therapeutic and Chemical (ATC) classification and Defined Daily Dose (DDD) developed by the World Health Organization (WHO) Collaborating Centre for Drug Statistics and the WHO Collaboration Centre for International Drug Consumption Monitoring. Human medicine importation data were collected from TMDA headquarters and used to assess the systemic antiviral and antifungal consumption trends. The importation data included the date, generic name, strength, brand name, currency, quantity, ATC classification, supplier country, port of entry and product supplier. The data were cleaned, reorganized and analyzed. Reference was made to the latest revised DDD list to assign antifungals and antivirals to their respective ATC/DDDs and then adjusted to the population estimates from the National Bureau of Statistics of Tanzania.

Results

There was a high proportion of systemic antivirals and antifungals utilization with 367.1 and 10.8 DDD per 1000 inhabitants per day (DID) respectively over eight years. In regression model, there was a significant increase in both antiviral (p-value = 0.043) and antifungal (p-value = 0.015) agents’ utilization trends in Tanzania in the study period. Fluconazole had the highest proportion of utilization for antifungals, followed by ketoconazole, itraconazole, miconazole and amphotericin B. For systemic antivirals, a high proportion was attributed to antiretrovirals used for HIV infections.

Conclusion

Findings from this study suggest an increase in the utilization of systemic antiviral and antifungal agents. These findings may be used to further benchmark utilization and AMR studies in Tanzania.
Secondary Intrusion Formation of Multiphase Plumes
Authors: Wang Dayang , Adams E. Eric
Published: October 2021

This work presents a new laboratory study for understanding secondary intrusions in multiphase plumes in quiescent, stratified environments. The study is driven by field observations of secondary intrusions during the Deepwater Horizon (DWH) oil spill. The smaller trap heights observed at DWH for secondary versus primary intrusions could have resulted, in part, from decreasing plume buoyancy (due to gas dissolution) and increasing ambient stratification with elevation above the source. We seek additional mechanisms responsible for the observed smaller secondary trap heights through controlled laboratory experiments where buoyancy and ambient stratification are nominally constant throughout. A novel approach is adopted in the experiments to increase the visibility of secondary intrusions, which are traditionally difficult to visualize, thus investigate. The study reveals that a wider plume source width can also cause the secondary intrusions to trap earlier, providing another plausible explanation for the shallower secondary intrusions observed in the field data.
The Native Dietary Habits of the Two Sympatric Bee Species and Their Effects on Shaping Midgut Microorganisms
Authors: Wang Ying , Li Zhenfang , Ma Lanting , Li Guilin , Han Kai , Liu Zhenguo , Wang Hongfang , Xu Baohua
Published: October 2021

The intestinal microbial community composition of different bee species typically has host specificity, yet little is known about the underlying formation mechanism. There are signs that dietary habits vary in different bee species, suggesting that there may be close relationships between dietary habits and intestinal microorganisms. We explored this hypothesis by comparing the dietary habits and gut microbiota of two common bee species (Apis mellifera L. and Apis cerana cerana) in China. Bee bread and midgut samples from wild and laboratory-reared bees were collected, and the differences in intestinal microbial community composition and growth and development before and after the change in dietary habits of different bee species were compared. We found that the two sympatric species had different dietary specializations and similar metagenomic diversities. The microbiota composition differed between the two species. Moreover, we revealed that changes in native dietary habits destroyed the intestinal microbiota community composition, negatively affecting the growth and development of honeybees.
Folic Acid Alleviates High Glucose and Fat-Induced Pyroptosis via Inhibition of the Hippo Signal Pathway on H9C2 Cells
Authors: Hong Lei , Zha Yingjie , Wang Chen , Qiao Shigang , An Jianzhong
Published: October 2021

Diabetic cardiomyopathy (DCM) is the leading cause of death in diabetic patients. Folic acid has a protective effect on diabetes-induced cardiomyocyte damage. The aim of this study was to explore the effects of folic acid on cardiomyocytes cultured under high glucose and fat (HGF) conditions and type 2 diabetes mellitus (T2DM) mice, and elucidate the underlying mechanisms. Bioinformatics analysis was used to identify the potential drugs through the Drug-Gene Interaction database. H9C2 cardiomyocytes were cultured with 30 mM glucose and 500 nM palmitic acid in the presence or absence of folic acid or YAP1 inhibitor (verteporfin) or YAP1 siRNA. The cell viability and lactate dehydrogenase (LDH) release were measured using specific assay kits. Pyroptosis was detected by flow cytometry. The concentrations of IL-1β and IL-18 in the supernatants were measured by ELISA. The NLRP3, ASC and caspase-1 mRNA levels were detected by qRT-PCR and that the proteins expression of NLRP3, ASC, cleaved caspase-1 (p10), caspase-1, YAP1, p-YAP1, LATS1 and P-LATS1 were detected by Western blotting. C57BL/6 mice were fed with high fat diet (HFD) combined with streptozotocin (STZ) intraperitoneally to establish a T2DM model, folic acid or PBS treatment for 8 weeks by oral gavage, blood glucose and body weight were measured every 4 weeks, mouse heart tissue was used to detect pyroptosis and hippo signaling pathway related protein expression. We identified 427 differentially expressed genes in the cardiac tissues of high fat diet + streptozotocin mice, among the 30 most significantly DEGs, folic acid was predicted to be the most likely therapeutic drug. Folic acid alleviated HGF-induced cell damage in vitro and in vivo by decreasing activation of the Hippo pathway, as indicated by lower LDH release and increased cell viability, and decreased expression of NLRP3, ASC, cleaved caspase-1, IL-1β, IL-18, p-YAP and p-LATS. Verteporfin or YAP1 siRNA neutralized the protective effect of folic acid by reversing YAP1-induced pyroptosis. Folic acid reduced NLRP3 inflammasome-mediated pyroptosis by down-regulating the Hippo signaling pathway, thereby effectively reducing T2DM-induced damage in H9C2 cells and animals.
Clinical Assessment of Visual Motion Perception in Children With Brain Damage: A Comparison With Base Rates and Control Sample
Authors: van der Zee Ymie J. , Stiers Peter L. J. , Lagae Lieven , Evenhuis Heleen M.
Published: October 2021

Aim: In this study, we examined (1) the presence of abnormally low scores (below 10th percentile) in various visual motion perception aspects in children with brain damage, while controlling for their cognitive developmental delay; (2) whether the risk is increased in comparison with the observation and expectation in a healthy control group and healthy population.

Methods: Performance levels of 46 children with indications of brain damage (Mage = 7y4m, SD = 2y4m) on three visual motion perception aspects (global motion, motion speed, motion-defined form) were evaluated. We used developmental age as entry of a preliminary reference table to classify the patient’s performance levels. Then we compared the percentages of abnormally low scores with percentages expected in the healthy population using estimated base rates and the observed percentages in the control sample (n = 119).

Results: When using developmental age as reference level, the percentage of low scores on at least one of the three tasks was significantly higher than expected in the healthy population [19/46, 41% (95%CI: 28–56%), p = 0.03]. In 15/19 (79% [95%CI: 61–97%] patients only one aspect of motion perception was affected. Four patients performed abnormally low on two out of three tasks, which is also higher than expected (4/46, 8.7%, 95%CI: 2.4–20.8% vs. 2.1%; z = 2.61, p < 0.01). The observed percentages in the patient group were also higher than found in the control group.

Interpretation: There is some evidence that children with early brain damage have an increased risk of isolated and combined motion perception problems, independent of their performance IQ.
Biofortification of Cereals and Pulses Using New Breeding Techniques: Current and Future Perspectives
Authors: Shahzad Rahil , Jamil Shakra , Ahmad Shakeel , Nisar Amina , Khan Sipper , Amina Zarmaha , Kanwal Shamsa , Aslam Hafiz Muhammad Usman , Gill Rafaqat Ali , Zhou Weijun
Published: October 2021

Cereals and pulses are consumed as a staple food in low-income countries for the fulfillment of daily dietary requirements and as a source of micronutrients. However, they are failing to offer balanced nutrition due to deficiencies of some essential compounds, macronutrients, and micronutrients, i.e., cereals are deficient in iron, zinc, some essential amino acids, and quality proteins. Meanwhile, the pulses are rich in anti-nutrient compounds that restrict the bioavailability of micronutrients. As a result, the population is suffering from malnutrition and resultantly different diseases, i.e., anemia, beriberi, pellagra, night blindness, rickets, and scurvy are common in the society. These facts highlight the need for the biofortification of cereals and pulses for the provision of balanced diets to masses and reduction of malnutrition. Biofortification of crops may be achieved through conventional approaches or new breeding techniques (NBTs). Conventional approaches for biofortification cover mineral fertilization through foliar or soil application, microbe-mediated enhanced uptake of nutrients, and conventional crossing of plants to obtain the desired combination of genes for balanced nutrient uptake and bioavailability. Whereas, NBTs rely on gene silencing, gene editing, overexpression, and gene transfer from other species for the acquisition of balanced nutritional profiles in mutant plants. Thus, we have highlighted the significance of conventional and NBTs for the biofortification of cereals and pulses. Current and future perspectives and opportunities are also discussed. Further, the regulatory aspects of newly developed biofortified transgenic and/or non-transgenic crop varieties via NBTs are also presented.
Comparison of Biochemical and Pathological Parameters and Parenteral Nutrition of ICU Patients Under Supervision of Dietitians and Surgeons
Authors: Behrad Nasab Mojgan , Akbari Mohammad Esmail , Rastgoo Samira , Gholami Somayeh , Hajipour Azadeh , Majidi Nazanin , Gholamalizadeh Maryam , Mirzaei Dahka Samaneh , Doaei Saied , Goodarzi Mark O.
Published: October 2021

Background: Nutrient imbalance can frequently occur in patients with indications for parenteral nutrition (PN) after gastrointestinal surgery. This study aimed to compare the recommendations of a surgeon to those of a dietitian in the field of parenteral nutrition.

Methods: This study was performed on 256 patients undergoing gastrointestinal surgery who received PN, which included 120 patients who received PN based on recommendations of the surgeons and 136 patients who were referred to receive PN under the supervision of a dietitian in Razi Hospital in Rasht, Iran. Data on PN and clinical outcomes of the patients were collected.

Results: Patients under the supervision of dietitians received higher vitamin B complex and lipids and lower vitamin A and vitamin E than the surgeon-supervised patients (all P < 0.001). In the group receiving PN under the supervision of a surgeon, the level of blood glucose (207 vs. 182, P < 0.01), sodium (138 vs. 136, P = 0.01), potassium (3.97 vs. 3.53, P < 0.01), and white blood cell count (9.83 vs. 9.28, P < 0.01) increased significantly at the end of the PN compared to baseline. In the group receiving PN under the supervision of a dietician, the level of serum Cr (1.23 vs. 1.32, P = 0.04), Mg (2.07 vs. 1.84, P < 0.01), and pH (7.45 vs. 7.5, P = 0.03) significantly improved after receiving parenteral nutrition compared to baseline.

Conclusion: The amounts of nutrients recommended for PN by the surgeon and dietitian were different. Implementation of dietitian recommendations in critically ill patients under PN can improve patients' clinical parameters.
90-Gene Expression Profiling for Tissue Origin Diagnosis of Cancer of Unknown Primary
Authors: Zhang Yi , Xia Lei , Ma Dawei , Wu Jing , Xu Xinyu , Xu Youtao
Published: October 2021

Cancer of unknown primary (CUP), in which metastatic diseases exist without an identifiable primary location, accounts for about 3–5% of all cancer diagnoses. Successful diagnosis and treatment of such patients are difficult. This study aimed to assess the expression characteristics of 90 genes as a method of identifying the primary site from CUP samples. We validated a 90-gene expression assay and explored its potential diagnostic utility in 44 patients at Jiangsu Cancer Hospital. For each specimen, the expression of 90 tumor-specific genes in malignant tumors was analyzed, and similarity scores were obtained. The types of malignant tumors predicted were compared with the reference diagnosis to calculate the accuracy. In addition, we verified the consistency of the expression profiles of the 90 genes in CUP secondary malignancies and metastatic malignancies in The Cancer Genome Atlas. We also reported a detailed description of the next-generation coding sequences for CUP patients. For each clinical medical specimen collected, the type of malignant tumor predicted and analyzed by the 90-gene expression assay was compared with its reference diagnosis, and the overall accuracy was 95.4%. In addition, the 90-gene expression profile generally accurately classified CUP into the cluster of its primary tumor. Sequencing of the exome transcriptome containing 556 high-frequency gene mutation oncogenes was not significantly related to the 90 genes analysis. Our results demonstrate that the expression characteristics of these 90 genes can be used as a powerful tool to accurately identify the primary sites of CUP. In the future, the inclusion of the 90-gene expression assay in pathological diagnosis will help oncologists use precise treatments, thereby improving the care and outcomes of CUP patients.
Detailed Analysis and Radiomic Prediction of First Progression Sites of First-Line Targeted Therapy for EGFR-Mutant Lung Adenocarcinoma Patients With Systemic Metastasis
Authors: Li Xiaoyang , Hou Runping , Yu Wen , Zhu Xueru , Li Hongwei , Yang Yidong , Qian Dong , Fu Xiaolong
Published: October 2021

Background

We aimed to analyze the first progression sites of first-line tyrosine kinase inhibitor (TKI) treatment for EGFR-mutant lung adenocarcinoma patients with systemic metastasis to recognize the potential candidates who might benefit from radiotherapy and establish a radiomic-based model to predict the first progression sites.

Materials and Methods

We retrospectively collected the clinical information and pre-treatment chest CT images of patients in Shanghai Chest Hospital from 2013 to 2017. All patients were diagnosed with stage IV EGFR-mutant lung adenocarcinoma and received TKI as first-line treatment. The first progression sites and survival were analyzed. The pre-treatment chest non-contrast CT images were utilized to establish a radiomic-based model to predict the first progression sites.

Results

We totally collected 233 patients with systemic metastasis, among whom, there were 84 (36.1%) and 149 (63.9%) patients developing first progression in original lesions (OP) and new lesions (NP), respectively. The PFS and OS of patients with OP were longer than those with NP (PFS 11 months vs. 8 months, p = 0.03, OS 50 months vs. 35 months, p = 0.046). For 67.9% of the patients with OF, disease progressed within five sites (oligoprogression). The radiomic-based model could predict the progression sites with an AUC value of 0.736, a specificity of 0.60, and a sensitivity of 0.750 in the independent validation set.

Conclusion

Among patients with systemic metastasis, there were 36.1% of patients developing OP at first progression who had a better prognosis than those developing NP. Patients with OP may be potential candidates who might benefit from radiotherapy. Radiomics is a useful method to distinguish patients developing OP and could provide some indications for radiotherapy.
Kaempferol Ameliorates the Inhibitory Activity of Dexamethasone in the Osteogenesis of MC3T3-E1 Cells by JNK and p38-MAPK Pathways
Authors: Xie Baocheng , Zeng Zhanwei , Liao Shiyi , Zhou Chenhui , Wu Longhuo , Xu Daohua
Published: October 2021

Kaempferol has been reported to exhibit beneficial effect on the osteogenic differentiation in mesenchymal stem cells (MSC) and osteoblasts. In our previous study, dexamethasone (DEX) demonstrated inhibitory effect on MC3T3-E1 cells differentiation. In this study, we mainly explored the protective effect of kaempferol on the inhibitory activity of DEX in the osteogenesis of MC3T3-E1 cells. We found that kaempferol ameliorated the proliferation inhibition, cell cycle arrest, and cell apoptosis and increased the activity of alkaline phosphatase (ALP) and the mineralization in DEX-treated MC3T3-E1 cells. Kaempferol also significantly enhanced the expression of osterix (Osx) and runt-related transcription factor 2 (Runx2) in MC3T3-E1 cells treated with DEX. In addition, kaempferol attenuated DEX-induced reduction of cyclin D1 and Bcl-2 expression and elevation of p53 and Bax expression. Kaempferol also activated JNK and p38-MAPK pathways in DEX-treated MC3T3-E1 cells. Furthermore, kaempferol improved bone mineralization in DEX-induced bone damage in a zebrafish larvae model. These data suggested that kaempferol ameliorated the inhibitory activity of DEX in the osteogenesis of MC3T3-E1 cells by activating JNK and p38-MAPK signaling pathways. Kaempferol exhibited great potentials in developing new drugs for treating glucocorticoid-induced osteoporosis.
Grey Relational Analysis Combined With Network Pharmacology to Identify Antioxidant Components and Uncover Its Mechanism From Moutan Cortex
Authors: Zhang Yingchun , Wu Xiaoyi , Wang Xinhui , Zeng Yue , Liao Yixuan , Zhang Ruizhi , Zhai Fuqiang , Zeng Zhongliang
Published: October 2021

The present study determines the potential antioxidants in Moutan Cortex (MC) and predicts its targets of anti-oxidative activities. The quantitative analysis and the free radical scavenging assays were conducted to detect the main components in MC and assess its anti-oxidant activities. The grey relational analysis and the network pharmacology approach were employed to predict its key components and targets of anti-oxidant activities. Six main constitutes in MCs were quantified by high performance liquid chromatography (HPLC) and its anti-oxidant activities were evaluated by DPPH and ABTS free radical scavenging methods. Then grey relational analysis was employed to predict the key components acting on anti-oxidative activity based on the chem-bio results. The predicted components and its mechanisms on anti-oxidation were uncovered by network pharmacology approach and cell test, respectively. The content of paeonol and paeoniflorin accounts for more than 80% the whole content of detected components. However, the two main ingredients showed a great variety among MCs. The antioxidant capacities of MCs also showed a great discrepancy based on DPPH and ABTS methods. The key components acting on anti-oxidation were identified to be paeonol, gallic acid and benzoylpaeoniflorin, and their potential therapeutic targets were predicted and verified, respectively. The present results reveal that MC has a significant antioxidant activity and the compounds of paeonol, gallic acid and benzoylpaeoniflorin could be considered as the promising antioxidant candidates with the property of suppressing oxidative stress and apoptosis.
Anti-flavivirus Properties of Lipid-Lowering Drugs
Authors: Farfan-Morales Carlos Noe , Cordero-Rivera Carlos Daniel , Reyes-Ruiz José Manuel , Hurtado-Monzón Arianna M. , Osuna-Ramos Juan Fidel , González-González Arely M. , De Jesús-González Luis Adrián , Palacios-Rápalo Selvin Noé , del Ángel Rosa María
Published: October 2021

Although Flaviviruses such as dengue (DENV) and zika (ZIKV) virus are important human pathogens, an effective vaccine or antiviral treatment against them is not available. Hence, the search for new strategies to control flavivirus infections is essential. Several studies have shown that the host lipid metabolism could be an antiviral target because cholesterol and other lipids are required during the replicative cycle of different Flaviviridae family members. FDA-approved drugs with hypolipidemic effects could be an alternative for treating flavivirus infections. However, a better understanding of the regulation between host lipid metabolism and signaling pathways triggered during these infections is required. The metabolic pathways related to lipid metabolism modified during DENV and ZIKV infection are analyzed in this review. Additionally, the role of lipid-lowering drugs as safe host-targeted antivirals is discussed.
New Insights on Structures Forming the Lignin-Like Fractions of Ancestral Plants
Authors: Rencoret Jorge , Gutiérrez Ana , Marques Gisela , del Río José C. , Tobimatsu Yuki , Lam Pui Ying , Pérez-Boada Marta , Ruiz-Dueñas Francisco Javier , Barrasa José M. , Martínez Angel T.
Published: October 2021

In the present work, lignin-like fractions were isolated from several ancestral plants –including moss (Hypnum cupressiforme and Polytrichum commune), lycophyte (Selaginella kraussiana), horsetail (Equisetum palustre), fern (Nephrolepis cordifolia and Pteridium aquilinum), cycad (Cycas revoluta), and gnetophyte (Ephedra fragilis) species– and structurally characterized by pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) and two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Py-GC/MS yielded marker compounds characteristic of lignin units, except in the H. cupressiforme, P. commune and E. palustre “lignins,” where they were practically absent. Additional structural information on the other five samples was obtained from 2D-NMR experiments displaying intense correlations signals of guaiacyl (G) units in the fern and cycad lignins, along with smaller amounts of p-hydroxyphenyl (H) units. Interestingly, the lignins from the lycophyte S. kraussiana and the gnetophyte E. fragilis were not only composed of G- and H-lignin units but they also incorporated significant amounts of the syringyl (S) units characteristic of angiosperms, which appeared much later in plant evolution, most probably due to convergent evolution. The latter finding is also supported by the abundance of syringol derivatives after the Py-GC/MS analyses of these two samples. Regarding lignin structure, β−O−4′ alkyl-aryl ethers were the most abundant substructures, followed by condensed β−5′ phenylcoumarans and β−β′ resinols (and dibenzodioxocins in the fern and cycad lignins). The highest percentages of alkyl-aryl ether structures correlated with the higher S/G ratio in the S. Kraussiana and E. fragilis lignin-like fractions. More interestingly, apart from the typical monolignol-derived lignin units (H, G and S), other structures, assigned to flavonoid compounds never reported before in natural lignins (such as amentoflavone, apigenin, hypnogenol B, kaempferol, and naringenin), could also be identified in the HSQC spectra of all the lignin-like fractions analyzed. With this purpose, in vitro synthesized coniferyl-naringenin and coniferyl-apigenin dehydrogenation polymers were used as standards. These flavonoids were abundant in H. cupressiforme appearing as the only constituents of the moss lignin-like fraction (including 84% of dimeric hypnogenol B) and their abundance decreased in those of S. Kraussiana (with amentoflavone and naringenin representing 14% of the total aromatic units), and the two ancient gymnosperms (0.4–1.2%) and ferns (0–0.7%).
Genome-Wide Association Mapping Identifies Novel Panicle Morphology Loci and Candidate Genes in Sorghum
Authors: Wang Lihua , Upadhyaya Hari D. , Zheng Jian , Liu Yanlong , Singh Shailesh Kumar , Gowda C. L. L. , Kumar Rajendra , Zhu Yongqun , Wang Yi-Hong , Li Jieqin
Published: October 2021

Panicle morphology is an important trait in racial classification and can determine grain yield and other agronomic traits in sorghum. In this study, we performed association mapping of panicle length, panicle width, panicle compactness, and peduncle recurving in the sorghum mini core panel measured in multiple environments with 6,094,317 single nucleotide polymorphism (SNP) markers. We mapped one locus each on chromosomes 7 and 9 to recurving peduncles and eight loci for panicle length, panicle width, and panicle compactness. Because panicle length was positively correlated with panicle width, all loci for panicle length and width were colocalized. Among the eight loci, two each were on chromosomes 1, 2, and 6, and one each on chromosomes 8 and 10. The two loci on chromosome 2, i.e., Pm 2-1 and Pm 2-2, were detected in 7 and 5 out of 11 testing environments, respectively. Pm 2-2 colocalized with panicle compactness. Candidate genes were identified from both loci. The rice Erect Panicle2 (EP2) ortholog was among the candidate genes in Pm 2-2. EP2 regulates panicle erectness and panicle length in rice and encodes a novel plant-specific protein with unknown functions. The results of this study may facilitate the molecular identification of panicle morphology-related genes and the enhancement of yield and adaptation in sorghum.
Feeling Free and Having an Authentic Inner Compass as Important Aspects of the Need for Autonomy in Emerging Adults’ Interactions With Their Mothers
Authors: Assor Avi , Cohen Rinat , Ezra Ohad , Yu Shi
Published: October 2021

Based on past theorizing and research, we posited that there are two kinds of specific experiences that contribute to the satisfaction of the general need for autonomy in emerging adults, as reflected in volitional, self-endorsed, actions. These experiences are: (1) feeling free, and (2) having a valid authentic inner compass (AIC). In the first study testing this hypothesis, college students in Israel (n= 163, mean age = 21.33), and in China (n= 72, mean age = 23.67) completed measures assessing experiences of freedom and having a valid AIC during contacts with mothers, extent of volitional contacts with mothers, and vitality during contacts with mothers. Confirmatory factor analyses and invariance analysis supported the validity of the measures, and their equivalence across cultures. In the Israeli sample, students also completed a measure assessing the extent to which the sense of having a valid AIC during contacts with one’s mother is based on intrinsic aspirations and goals. As expected, across cultures, participants distinguished between experiences of (a) having a valid AIC, and (b) feeling free. The findings also suggest that experiences of freedom and of having a valid AIC during contacts with mothers are associated with extent of volitional contacts with mother, and subsequent vitality during these contacts. Also as expected, experiencing a valid AIC during contacts with mother was associated with volitional contacts with her and subsequent vitality - only when the AIC was based on intrinsic aspirations. The results suggest that in assessing people’s sense of AIC, it is important to consider the content of the aspirations and goals on which this sense is based. The findings are consistent with the view that feeling free, and having a valid AIC are two specific autonomy experiences which promote a more global experience of need autonomy satisfaction, as indicated by feelings of volitional and self-endorsed action.
Father-Child Conflict and Chinese Adolescent Depression: A Moderated Mediation Model
Authors: Peng Cong , Chen Jianwen , Wu Huifen , Liu Yan , Liao Youguo , Wu Yuqin , Zheng Xintong
Published: October 2021

To investigate the effects of father-child conflict and regulatory emotional self-efficacy (RESE) on Chinese adolescent depression, 654 middle-school students were measured. The results showed that: (1) Father-son conflict was significantly lower than father-daughter conflict, girls’ depression was significantly higher than that of boys, and boys’ RESE and self-efficacy in regulating negative emotions (NEG) were significantly higher than that for girls, but there was no significant difference between boys and girls in self-efficacy in expressing positive emotions (POS). (2) Father-child conflict was significantly positively associated with Chinese adolescent depression. Father-child conflict was negatively correlated with RESE, and its two dimensions. Both POS and NEG played a partial mediating role in the relationship between father-child conflict and adolescent depression. (3) Gender only regulated the relationship between NEG and adolescent depression. Compared to boys, girls are more affected by depression at the low level of NEG.
Time Perception in Prodromal Alzheimer's Dementia and in Prodromal Dementia With Lewy Bodies
Authors: Pai Ming-Chyi , Yang Chiu-Jun , Fan Sheng-Yu
Published: October 2021

Background: Time perception is a subjective experience or sense of time. Previous studies have shown that Alzheimer's dementia (AD) patients have time perception deficits compared to a cognitively unimpaired control group (CU). There are only a few studies on dementia with Lewy bodies (DLB) patients' time perception in comparison with CU and AD patients. Early intervention and prescription of the right medicine may delay the deterioration of AD and DLB, moreover, knowing how prodromal AD (prAD) and prodromal DLB's (prDLB) time perception differ from each other might be helpful for future understanding of these two dementias. Therefore, the purpose of this study is to explore the difference in time perception performance between prodromal AD and prodromal DLB.

Methods: We invited people diagnosed with prAD, prDLB, and CU to participate in this study. Tests of verbal estimation of time and time interval production were used to assess their time perception. We analyzed the average time estimation (ATE), absolute error score (ABS), coefficient of variance (CV), and subjective temporal unit (STU) within the three groups.

Results: A total of 40 prAD, 30 prDLB, and 47 CU completed the study. In the verbal estimation test, the CV for the prAD was higher than both prDLB and CU at the 9 s interval, and the CV of prAD was higher than CU at the 27 s interval. In the time interval production test, the subjective time units of prDLB were higher than prAD at the 10 s interval, while those of both prDLB and CU were higher than prAD at the 30 s interval. The percentage of subjects with STU < 1.0 s, indicating overestimation, was higher in prAD than both prDLB and CU.

Conclusion: Time perception of prAD patients showed imprecision and overestimation of time, while prDLB tended to underestimate time intervals. No significant difference was found in accuracy among the three groups. It is speculated that the clinical and pathological severity of the two prodromal dementia stages may be different, and some patients have not yet had their time perception affected.
Sexuality in Persons With Hidradenitis Suppurativa: Factors Associated With Sexual Desire and Functioning Impairment
Authors: Quinto Rossella Mattea , Mastroeni Simona , Sampogna Francesca , Fania Luca , Fusari Roberta , Iani Luca , Abeni Damiano
Published: October 2021

Hidradenitis Suppurativa (HS) is a chronic skin disease involving intimate and sensitive areas and affecting physical and mental health. We investigated the prevalence of sexual desire and functioning impairment, and their associations with quality of life, anxiety, depression, minor psychiatric disorders (MPD), and clinical features (e.g., disease severity) in 77 patients with HS who completed self-report measures and answered to questions assessing socio-demographic characteristics, lifestyle habits, and hindered sexuality due to HS. The majority of patients reported hindered sexuality, and poor sexual functioning, while showing good levels of dyadic and solitary sexual desire. No associations were found between clinical severity and sexuality measures. Multivariate analyses showed significant associations of sexual outcome measures with alcohol consumption, low Body Mass Index, family history of HS, and severe skin symptoms. Moreover, we found that the presence of negative psychological factors (i.e., MPD, anxiety, poor mental status) increased the risk of sexual impairment. These findings underline the important role of psychological and sexual aspects in HS patients and suggest that physicians should consider the effect of disease burden on patients' sexual health.
COVID-19 Pandemic & Bureaucracy: The Crisis Inside the Crisis
Authors: Roßmann Katalyn , Wegner Heike , Stark Hans , Großmann Gerd , Jansen Andreas , Frangoulidis Dimitrios
Published: October 2021

The Medical Intelligence and Information (MI2) Unit of the German Armed Forces (Bundeswehr) is experienced in crisis support in military missions since several years. It gained additional experiences during the current coronavirus 2019 (COVID-19) pandemic on different levels of the response to crisis and was requested to share the findings and expertise with the overloaded civil public health agencies inside Germany. Since the beginning of the pandemic, the unit is constantly developing new products for crisis communication, knowledge sharing techniques in new databases, dashboards for leadership, and training for laypersons in contact tracing. Hence, trying to innovate in crisis since the first severe acute respiratory syndrome coronavirus (SARS-CoV)-2-disease wave. During the second wave, the unit was requested to evaluate the outbreak management of different national civil public health agencies in southern Germany, and to support the development of dashboards in a comprehensive public health approach as a necessary start toward digitalization.
Prevalence and Recurrence Rates of Cytomegalovirus Infection Among Patients With Hematological Diseases in the Western Brazilian Amazon: A Cross-Sectional Study
Authors: de Melo Silva Jean , Pinheiro-Silva Renato , Costa de Oliveira Regiane , de Castro Alves Carlos Eduardo , Barbosa Anderson Nogueira , Pontes Gemilson Soares
Published: October 2021

Cytomegalovirus (CMV) is a worldwide distributed pathogen that may cause serious complications in patients with hematological diseases. This study aimed to serologically characterize CMV infection in patients suffering from hematological diseases in Amazonas state, Brazil. Serum samples from 323 patients were tested for the presence of anti-CMV IgM or IgG antibodies using an enzyme-linked immunosorbent assay. Positive samples for IgM were submitted to the IgG avidity test to differentiate primary infection from recurrent infection. An epidemiological questionnaire was administered to collect the sociodemographic information of the study population. The overall prevalence of CMV infection verified in this study was 91.3%. The highest rates were found in patients suffering from platelet disorders (94.5%), anemia (93.3%), or leukemia (91%). The study population was predominantly composed of individuals with low socioeconomic status. Blood transfusions were more common in patients with anemia or leukemia, but this variable was not correlated with the seropositivity for CMV infection. Measurement of IgG avidity in patients positive for anti-CMV IgM demonstrated a recurrent infection rate of 5.2% (17/323). Over 80% of recurrent infections occurred in patients with acute lymphocytic leukemia (ALL) or anemia. Our findings indicated that CMV infection is highly prevalent in patients from the western Brazilian Amazon who have hematological diseases. The prevalence observed progressively rose with increasing age, whereas anemia or ALL figured as risk factors for the recurrence of CMV infection.
Human Papillomavirus Vaccine Course Completion Among Gay and Bisexual Men Who Have Sex With Men From a Time-Limited HPV Vaccination Catch-Up Program in Victoria, Australia
Authors: Htaik Kay , Fairley Christopher K. , Chen Marcus Y. , Wigan Rebecca , Rodriguez Elena , Bradshaw Catriona S. , Chow Eric P. F.
Published: October 2021

Background: To examine completion of the human papillomavirus (HPV) vaccination 3-dose regimen and factors associated with completion among men who have sex with men (MSM) aged ≤ 26 years participating in a time-limited HPV catch-up vaccination program in Victoria, Australia.

Methods: MSM who received their first dose of HPV vaccine at Melbourne Sexual Health Centre in 2017 were followed until October 2019. Vaccination completion was defined as those who received three doses. Multivariable logistic regression was performed to examine factors associated with vaccine completion.

Results: 931 of 1,947 (47.8%) eligible men received at least one dose of HPV vaccine, 750 (38.5%) received two and 590 (30.3%) received three doses. The median time to receiving the second and third dose was 2.8 (IQR = 2.1–4.8) and 7.2 (IQR = 6.3–10.7) months, respectively. Gay men had higher odds of receiving three doses compared to bisexual men (aOR = 2.17; 95%CI: 1.16–4.04). Compared with HIV-negative MSM not taking PrEP, HIV-positive MSM were more like to complete vaccination (aOR = 3.92, 95%CI: 1.62–9.47) but no difference was found compared to HIV-negative men taking PrEP (aOR = 1.55; 95%CI: 0.95–2.53).

Conclusion: Less than one-third of men aged ≤ 26 years completed the three doses of HPV vaccine. Further studies are needed to understand the barriers of men not completing the vaccine.
XIX-wieczny słowniczek gwarowy z Podlasia Zygmunta
Wasilewskiego jako przyczynek do dziejów polskiej leksykografi i gwarowej .
Authors: Cygan Stanisław
Published: October 2021

The subject matter described in this paper is the lexicographic work of
Zygmunt Wasilewski, a lawyer, politician, columnist, literary critic, which refers
to his 19th-century glossary from the Podlasie region.
When characterising this small collection of words (282 headwords), the
macrostructure and microstructure of the glossary is taken into consideration.
Its lexical material is fi rst of all a source of knowledge about the oldest state
of Podlasie dialects and research on Polish dialects overall, but it also offers
an insight in the methods of this unqualifi ed lexicographer, enthusiast of “folk
things”, amateur ethnographer and dialectologist.
Phase Transformation and Characterization of 3D Reactive Microstructures in Nanoscale Al/Ni Multilayers
Authors: Sauni Camposano Yesenia Haydee , Riegler Sascha Sebastian , Jaekel Konrad , Schmauch Jörg , Pauly Christoph , Schäfer Christian , Bartsch Heike , Mücklich Frank , Gallino Isabella , Schaaf Peter
Published: October 2021

Reactive multilayer systems represent an innovative approach for potential usage in chip joining applications. As there are several factors governing the energy release rate and the stored chemical energy, the impact of the morphology and the microstructure on the reaction behavior is of great interest. In the current work, 3D reactive microstructures with nanoscale Al/Ni multilayers were produced by alternating deposition of pure Ni and Al films onto nanostructured Si substrates by magnetron sputtering. In order to elucidate the influence of this 3D morphology on the phase transformation process, the microstructure and the morphology of this system were characterized and compared with a flat reactive multilayer system on a flat Si wafer. The characterization of both systems was carried out before and after a rapid thermal annealing treatment by using scanning and transmission electron microscopy of the cross sections, selected area diffraction analysis, and differential scanning calorimetry. The bent shape of multilayers caused by the complex topography of silicon needles of the nanostructured substrate was found to favor the atomic diffusion at the early stage of phase transformation and the formation of two intermetallic phases Al0.42Ni0.58 and AlNi3, unlike the flat multilayers that formed a single phase AlNi after reaction.
Review on the Integration of Phase Change Materials in Building Envelopes for Passive Latent Heat Storage
Authors: Sawadogo Mohamed , Duquesne Marie , Belarbi Rafik , Hamami Ameur El Amine , Godin Alexandre
Published: October 2021

Latent heat thermal energy storage systems incorporate phase change materials (PCMs) as storage materials. The high energy density of PCMs, their ability to store at nearly constant temperature, and the diversity of available materials make latent heat storage systems particularly competitive technologies for reducing energy consumption in buildings. This work reviews recent experimental and numerical studies on the integration of PCMs in building envelopes for passive energy storage. The results of the different studies show that the use of PCMs can reduce the peak temperature and smooth the thermal load. The integration of PCMs can be done on the entire building envelope (walls, roofs, windows). Despite many advances, some aspects remain to be studied, notably the long-term stability of buildings incorporating PCMs, the issues of moisture and mass transfer, and the consideration of the actual use of the building. Based on this review, we have identified possible contributions to improve the efficiency of passive systems incorporating PCMs. Thus, fatty acids and their eutectic mixtures, combined with natural insulators, such as vegetable fibers, were chosen to make shape-stabilized PCMs composites. These composites can be integrated in buildings as a passive thermal energy storage material.
Experimental Measurement of Residual Stress Distribution in Rail Specimens Using Ultrasonic LCR Waves
Authors: Hwang Young-In , Kim Geonwoo , Kim Yong-Il , Park Jeong-Hak , Choi Man Yong , Kim Ki-Bok
Published: October 2021

Longitudinal critically refracted (LCR) waves are considered bulk longitudinal waves and penetrate into an effective depth beneath the surface parallel to the material surface. Such LCR waves can be employed to measure residual stresses because the acoustoelastic effect is the basis for ultrasonic residual stress measurements. This effect is described by the relationship between change of wave travel time and stress applied when such waves propagate in a stressed medium. In this paper, stresses applied in a rail were evaluated by using a developed LCR probe. With this transducer, it was verified how the difference in the arrival times of the LCR waves showed a trend as the tensile stresses increased. The acoustoelastic coefficients were calculated using the relationship between the stresses and the travel times, and the residual stresses of the used rails were measured using these coefficients. In addition, the difference in residual stress distribution according to the characteristics of the wheel-rail contact surface was analyzed from the obtained residual stress value. It was concluded that this non-destructive evaluation technique using LCR waves could be employed for accurate stress measurement of rails because differences in stress applied to the rail can be detected.