bluuebunny/crossref_metadata_2025 huggingface dataset:
Cross-Cultural Translation and Adaptation of the Consumer Ear Disease Risk Assessment (CEDRA) Questionnaire in Danish
Authors: Siggaard Lene Dahl , Jacobsen Henrik , Hougaard Dan Dupont , Khaled Mina Shereen , Høgsbro Morten
Published: November 2023

This study aimed to cross-culturally translate and adapt the Consumer Ear Disease Risk Assessment (CEDRA) questionnaire into Danish for remote ear, nose, and, throat assessments in adult, first-time hearing aid users when used in conjunction with audiometric measures and visual images of the tympanic membrane. Employing field-specific guidelines, the tool underwent a rigorous translation process. This was succeeded by field testing via cognitive debriefing with 30 intendent respondents and a pilot test involving 600 adult, potential first-time hearing aid users from 2020–2022. Test–retest reliability analysis in 113 respondents revealed high consistency and reproducibility, with most items showing Spearman’s correlation coefficients of 0.82 or higher and a Pearson’s correlation of 0.92 for the total score. The tool demonstrated moderate discriminative ability in identifying individuals at high and low risk of complicated hearing loss and targeted ear diseases, supported by an area under the curve of 0.82 on the receiver operating characteristics curve. Our findings suggest that the Danish-translated version of CEDRA is a reliable and effective screening instrument when used with audiometry and tympanometry, warranting further validation in a larger population.
Geometrically Non-Linear Plane Elasticity Problem in the Area of an Angular Boundary Cut-Out
Authors: Frishter Lyudmila
Published: November 2023

A relevant problem in the development and improvement of numeric analytical methods for the research of structures, buildings and construction is studying the stress–strain state of structures and construction with boundaries that have complex shapes. Deformations and stresses arise in a domain with a geometrically non-linear shape of the boundary (cut-outs and cuts). These stresses and deformations have great values and gradients. Experiments carried out using the photoelasticity method show a change in the deformation order ratios for different subareas of the boundary cut-out area depending on proximity to the apex of the angular cut-out. Areas with minor deformations are observed, and areas where linear deformations and shears are more significant than rotations are also observed. In addition, areas where section rotations are more significant than linear and shear deformations are observed. According to the experimental data, the mathematical model of the SSS in the area of the apex of the cut-out of the domain boundary should take into account non-linear deformations. Hence, it is necessary to formulate the boundary value problem of the theory of elasticity, taking into account the geometrical non-linearity. The research aim of this paper is to formulate the problem of the elasticity theory taking into account the geometrical non-linearity in furtherance of the proposed mathematical model justified by the experimental data obtained using the photoelasticity method. The obtained formulation of the elasticity theory problem allows analyzing the form of the system of equations of the boundary value problem depending on the proximity of the considered area to the irregular point of the boundary, i.e., taking into account the difference in the effect of linear and shear deformations, rotations and forced deformations on the solution to the geometrically non-linear elastic problem dealing with forced deformations in the area of an angular cut-out of the boundary of the plane domain.
Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology
Authors: Dowling Paul , Swandulla Dieter , Ohlendieck Kay
Published: November 2023

Voluntary striated muscles are characterized by a highly complex and dynamic proteome that efficiently adapts to changed physiological demands or alters considerably during pathophysiological dysfunction. The skeletal muscle proteome has been extensively studied in relation to myogenesis, fiber type specification, muscle transitions, the effects of physical exercise, disuse atrophy, neuromuscular disorders, muscle co-morbidities and sarcopenia of old age. Since muscle tissue accounts for approximately 40% of body mass in humans, alterations in the skeletal muscle proteome have considerable influence on whole-body physiology. This review outlines the main bioanalytical avenues taken in the proteomic characterization of skeletal muscle tissues, including top-down proteomics focusing on the characterization of intact proteoforms and their post-translational modifications, bottom-up proteomics, which is a peptide-centric method concerned with the large-scale detection of proteins in complex mixtures, and subproteomics that examines the protein composition of distinct subcellular fractions. Mass spectrometric studies over the last two decades have decisively improved our general cell biological understanding of protein diversity and the heterogeneous composition of individual myofibers in skeletal muscles. This detailed proteomic knowledge can now be integrated with findings from other omics-type methodologies to establish a systems biological view of skeletal muscle function.
Leucine Supplementation Improves Diastolic Function in HFpEF by HDAC4 Inhibition
Authors: Alves Paula Ketilly Nascimento , Schauer Antje , Augstein Antje , Männel Anita , Barthel Peggy , Joachim Dirk , Friedrich Janet , Prieto Maria-Elisa , Moriscot Anselmo Sigari , Linke Axel , Adams Volker
Published: November 2023

Heart failure with preserved ejection fraction (HFpEF) is a complex syndrome associated with a high morbidity and mortality rate. Leucine supplementation has been demonstrated to attenuate cardiac dysfunction in animal models of cachexia and heart failure with reduced ejection fraction (HFrEF). So far, no data exist on leucine supplementation on cardiac function in HFpEF. Thus, the current study aimed to investigate the effect of leucine supplementation on myocardial function and key signaling pathways in an established HFpEF rat model. Female ZSF1 rats were randomized into three groups: Control (untreated lean rats), HFpEF (untreated obese rats), and HFpEF_Leu (obese rats receiving standard chow enriched with 3% leucine). Leucine supplementation started at 20 weeks of age after an established HFpEF was confirmed in obese rats. In all animals, cardiac function was assessed by echocardiography at baseline and throughout the experiment. At the age of 32 weeks, hemodynamics were measured invasively, and myocardial tissue was collected for assessment of mitochondrial function and for histological and molecular analyses. Leucine had already improved diastolic function after 4 weeks of treatment. This was accompanied by improved hemodynamics and reduced stiffness, as well as by reduced left ventricular fibrosis and hypertrophy. Cardiac mitochondrial respiratory function was improved by leucine without alteration of the cardiac mitochondrial content. Lastly, leucine supplementation suppressed the expression and nuclear localization of HDAC4 and was associated with Protein kinase A activation. Our data show that leucine supplementation improves diastolic function and decreases remodeling processes in a rat model of HFpEF. Beneficial effects were associated with HDAC4/TGF-β1/Collagenase downregulation and indicate a potential use in the treatment of HFpEF.
Preparation of Modified Biochar and Its Adsorption of Cr(VI) in Aqueous Solution
Authors: Zuo Jinlong , Li Wenjing , Xia Zhi , Zhao Tingting , Tan Chong , Wang Yuyang , Li Junsheng
Published: November 2023

In recent years, wastewater containing heavy metal chromium has been discharged into water bodies. Metal chromium not only destroys the water environment but also poses a threat to human life and health. In order to solve the problem of chromium pollution more effectively, this study used corn straw as raw material to prepare biochar (MB) and used three methods: acid (HCl), alkali (NaOH) and metal salt (FeCl3) to modify biochar (HMB, NaMB and FeMB) and investigated the strengthening effect of modified biochar on Cr(VI) adsorption. The morphology and surface chemical composition of biochar were studied by XRD, SEM, XPS, FITR and other characterization methods. It was found that the modification of HCl, NaOH and FeCl3 improved the physical and chemical properties of MB (such as crystal structure, specific surface area, pore size and surface composite film), thus promoting the adsorption of Cr(VI). At the same time, an adsorption single-factor experiment, adsorption kinetics experiment, isothermal adsorption experiment and cyclic regeneration experiment were carried out on the four adsorbents. The effects of biochar on Cr(VI) adsorption performance under different pH, Cr(VI) initial concentration, biochar dosage and time were compared, and the adsorption mechanism of four adsorbents on Cr(VI) in aqueous solution was studied. It was found that the FeCl3-modified biochar provided more adsorption sites for chromium ions due to the successful loading of Fe, Fe3+ and iron oxide particles onto the MB surface to form a composite film, and the Fe-O groups introduced by the composite film formed a coordinated adsorption with dichromate ions. At 25 °C and pH = 2, FeMB reached saturation at 1440 min, the maximum adsorption capacity was 23.4 mg/g and its removal rate of Cr(VI) remained above 45% after five cycles. The adsorption of Cr(VI) was significantly enhanced.
A Simulated-Annealing-Quasi-Oppositional-Teaching-Learning-Based Optimization Algorithm for Distributed Generation Allocation
Authors: Taheri Seyed Iman , Davoodi Mohammadreza , Ali Mohd. Hasan
Published: November 2023

Conventional evolutionary optimization techniques often struggle with finding global optima, getting stuck in local optima instead, and can be sensitive to initial conditions and parameter settings. Efficient Distributed Generation (DG) allocation in distribution systems hinges on streamlined optimization algorithms that handle complex energy operations, support real-time decisions, adapt to dynamics, and improve system performance, considering cost and power quality. This paper proposes the Simulated-Annealing-Quasi-Oppositional-Teaching-Learning-Based Optimization Algorithm to efficiently allocate DGs within a distribution test system. The study focuses on wind turbines, photovoltaic units, and fuel cells as prominent DG due to their growing usage trends. The optimization goals include minimizing voltage losses, reducing costs, and mitigating greenhouse gas emissions in the distribution system. The proposed algorithm is implemented and evaluated on the IEEE 70-bus test system, with a comparative analysis conducted against other evolutionary methods such as Genetic Algorithm (GA), Particle Swarm Optimization (PSO), Honey Bee Mating Optimization (HBMO), and Teaching-Learning-Based Optimization (TLBO) algorithms. Results indicate that the proposed algorithm is effective in allocating the DGs. Statistical testing confirms significant results (probability < 0.1), indicating superior optimization capabilities for this specific problem. Crucially, the proposed algorithm excels in both accuracy and computational speed compared to other methods studied.
Numerical Approximations of Diblock Copolymer Model Using a Modified Leapfrog Time-Marching Scheme
Authors: Chen Lizhen , Ma Ying , Ren Bo , Zhang Guohui
Published: November 2023

An efficient modified leapfrog time-marching scheme for the diblock copolymer model is investigated in this paper. The proposed scheme offers three main advantages. Firstly, it is linear in time, requiring only a linear algebra system to be solved at each time-marching step. This leads to a significant reduction in computational cost compared to other methods. Secondly, the scheme ensures unconditional energy stability, allowing for a large time step to be used without compromising solution stability. Thirdly, the existence and uniqueness of the numerical solution at each time step is rigorously proven, ensuring the reliability and accuracy of the method. A numerical example is also included to demonstrate and validate the proposed algorithm, showing its accuracy and efficiency in practical applications.
Study of the Structural-Phase State of Hydroxyapatite Coatings Obtained by Detonation Spraying at Different O2/C2H2 Ratios
Authors: Baizhan Daryn , Sagdoldina Zhuldyz , Buitkenov Dastan , Kambarov Yedilzhan , Nabioldina Aiym , Zhumabekova Venera , Bektasova Gulsym
Published: November 2023

This work studies the influence of the composition of an acetylene–oxygen explosive O2/C2H2 mixture on the structure and properties of hydroxyapatite coatings obtained by detonation spraying. The molar ratios of O2/C2H2 were 2.61; 3.03 and 3.35; the explosive charge was between 73 and 77%. The results of X-ray phase analysis showed partial conversion of the hydroxyapatite (HA) phase to the tricalcium phosphate (α-TCP) phase and formation of the amorphous phase during detonation sputtering. The formation of a small amount of the α-TCP phase during detonation spraying of HA is obviously due to structural transformations occurring during the heating of the material by detonation products. In addition, very rapid cooling of molten particles leads to the formation of the amorphous phase. The study results of the microstructure of the cross sections of the formed coatings, conducted using scanning electron microscopy, indicate that an increase in the O2/C2H2 ratio leads to increased porosity in the coatings. Additionally, an increase in the explosive charge by 77% results in the appearance of transverse cracks in the coating.
The Characteristics of Light (TiCrAl0.5NbCu)CxNy High-Entropy Coatings Deposited Using a HiPIMS/DCMS Technique
Authors: Zoita Nicolae C. , Dinu Mihaela , Parau Anca C. , López-Ortega Ainara , Pana Iulian , Grigorescu Cristiana Eugenia Ana , Mondragon Mikel , Sobetkii Arcadie , Almandoz Xanti , Rodriguez Juan Carlos , Harb Alaa Abou , Kiss Adrian E. , Izurrategi Jose Manuel
Published: November 2023

Multi-component high-entropy (TiCrAl0.5NbCu)CxNy coatings targeting applications requiring medium-to-high friction and wear-resistant surfaces were fabricated through the co-sputtering of elemental targets in an Ar + CH4 + N2 reactive atmosphere using a hybrid HiPIMS/DCMS technique. Two sets of samples were fabricated: (a) (TiCrAl0.5NbCu)Cx high-entropy carbides (HEC) and (b) (TiCrAl0.5NbCu)CxN0.13 high-entropy carbonitrides (HECN), 0 ≤ x ≤ 0.48. The structural, mechanical, tribological, and corrosion resistance properties were thoroughly investigated. The metallic sample exhibits a single BCC structure that changes to FCC via an intermediary amorphous phase through the addition of C or N to the content of the films. The crystallinity of the FCC phases is enhanced and the density of the films decreases down to 5.5 g/cm3 through increasing the carbon fraction up to 48%. The highest hardness of about 16.9 GPa and the lowest wear rate of about 5.5 × 10−6 mm3/Nm are presented by the samples with the largest carbon content, x = 0.48. We found a very good agreement between the evolution of H/E and H3/E2 parameters with carbon content and the tribological behavior of the coatings. The best corrosion resistance was presented by the low-carbon carbonitride samples, showing a charge transfer resistivity of about 3 × 108 Ω∙cm, which is more than three times larger than that of the metallic HEA. The best tribological characteristics for envisioned application were presented by (TiCrAl0.5NbCu)C0.3N0.13, showing a coefficient of friction of 0.43 and a wear rate of about 7.7 × 10−6 mm3/Nm.
Deprescribing: A Prime Opportunity to Optimize Care of Cancer Patients
Authors: Chaput Genevieve , Bhanabhai Hitesh
Published: November 2023

Patients with incurable cancers have an increasing number of comorbidities, which can lead to polypharmacy and its associated adverse events (drug-to-drug interaction, prescription of a potentially inappropriate medication, adverse drug event). Deprescribing is a patient-centered process aimed at optimizing patient outcomes by discontinuing medication(s) deemed no longer necessary or potentially inappropriate. Improved patient quality of life, risk reduction of side effects or worse clinical outcomes, and a decrease in healthcare costs are well-documented benefits of deprescribing. Deprescribing and advance care planning both require consideration of patients’ values, preferences, and care goals. Here, we provide an overview of comorbidities and associated polypharmacy risks in cancer patients, as well as useful tools and resources for deprescribing in daily practice, and we shed light on how deprescribing can facilitate advance care planning discussions with patients who have advanced cancer or a limited life expectancy.
Pathogenetic Mechanisms Underlying Major Adverse Cardiac Events in Personality Type D Patients after Percutaneous Coronary Intervention: The Roles of Cognitive Appraisal and Coping Strategies
Authors: Sumin Alexey N. , Shcheglova Anna V.
Published: November 2023

Background: This paper aimed to study the association of type D personality, coping strategies, and cognitive appraisal with annual prognosis after a percutaneous coronary intervention (PCI) in patients with coronary artery disease (CAD). Methods: The prospective study included 111 CAD patients who underwent a PCI. All participants, before the PCI, completed questionnaires designed to collect information about type D personality, cognitive appraisal, and coping styles. Information was also collected on the clinical and demographic characteristics of the patients. After 1 year of follow-up, the presence of major adverse cardiac events (MACEs) was assessed. Results: The presence of a MACE was noted in 38 patients, and the absence of a MACE was noted in 53 patients. In patients with type D personality, higher incidences of MACEs (54.1% versus 33.3%; p = 0.0489) and hospitalization rates (29.7% versus 7.4%; p = 0.004) were revealed. Patients with poor prognoses preferred a moderate use of the confrontation strategy than patients without a MACE (78.4% vs. 50.9%; p = 0.0082). Patients with MACEs had statistically significantly lower indicators of strong emotions (11.92 ± 5.32 versus 14.62 ± 4.83 points; p = 0.005) and future prospects (11.36 ± 3.81 versus 13.21 ± 3.41 points; p = 0.015) than patients without a MACE. In a multiple binary logistic regression model, the following factors had significant associations with MACE development: type D, moderate use of confrontation coping, moderate use of self-control coping, and strong emotions in cognitive appraisal. Conclusion: This study showed that not only personality type D, but also certain coping strategies and cognitive appraisals increase the likelihood of developing a MACE after a PCI. This provides a theoretical basis for understanding the mechanism underlying type D personality and MACEs in patients after a PCI.
Eco-Friendly and COVID-19 Friendly? Decreasing the Carbon Footprint of the Operating Room in the COVID-19 Era
Authors: Tsagkaris Christos , Saeed Hamayle , Laubscher Lily , Eleftheriades Anna , Stavros Sofoklis , Drakaki Eirini , Potiris Anastasios , Panagiotopoulos Dimitrios , Sioutis Dimos , Panagopoulos Periklis , Zil-E-Ali Ahsan
Published: November 2023

Surgery is one of the most energy-intensive branches of healthcare. Although the COVID-19 pandemic has reduced surgical volumes, infection control protocols have increased the ecological footprint of surgery owing to the extensive use of personal protective equipment, sanitation, testing and isolation resources. The burden of environmental diseases requiring surgical care, the international commitment towards environmental sustainability and the global efforts to return to the pre-pandemic surgical workflow call for action towards climate-friendly surgery. The authors have searched the peer-reviewed and gray literature for clinical studies, reports and guidelines related to the ecological footprint of surgical care and the available solutions and frameworks to reduce it. Numerous studies concede that surgery is associated with a high rate of energy utilization and waste generation that is comparable to major non-medical sources of pollution. Recommendations and research questions outlining environmentally sustainable models of surgical practices span from sanitation and air quality improvement systems to the allocation of non-recyclable consumables and energy-efficient surgical planning. The latter are particularly relevant to infection control protocols for COVID-19. Paving the way towards climate-friendly surgery is a worthy endeavor with a major potential to improve surgical practice and outcomes in the long term.
A Motion Deblurring Network for Enhancing UAV Image Quality in Bridge Inspection
Authors: Lee Jin-Hwan , Gwon Gi-Hun , Kim In-Ho , Jung Hyung-Jo
Published: November 2023

Unmanned aerial vehicles (UAVs) have been increasingly utilized for facility safety inspections due to their superior safety, cost effectiveness, and inspection accuracy compared to traditional manpower-based methods. High-resolution images captured by UAVs directly contribute to identifying and quantifying structural defects on facility exteriors, making image quality a critical factor in achieving accurate results. However, motion blur induced by external factors such as vibration, low light conditions, and wind during UAV operation significantly degrades image quality, leading to inaccurate defect detection and quantification. To address this issue, this research proposes a deblurring network using a Generative Adversarial Network (GAN) to eliminate the motion blur effect in UAV images. The GAN-based motion deblur network represents an image inpainting method that leverages generative models to correct blurry artifacts, thereby generating clear images. Unlike previous studies, this proposed approach incorporates deblur and blur learning modules to realistically generate blur images required for training the generative models. The UAV images processed using the motion deblur network are evaluated using a quality assessment method based on local blur map and other well-known image quality assessment (IQA) metrics. Moreover, in the experiment of crack detection utilizing the object detection system, improved detection results are observed when using enhanced images. Overall, this research contributes to improving the quality and accuracy of facility safety inspections conducted with UAV-based inspections by effectively addressing the challenges associated with motion blur effects in UAV-captured images.
Combination of a Nondestructive Testing Method with Artificial Neural Network for Determining Thickness of Aluminum Sheets Regardless of Alloy’s Type
Authors: Mayet Abdulilah Mohammad , Shah Muhammad Umer Hameed , Hanus Robert , Loukil Hassen , Parayangat Muneer , Muqeet Mohammed Abdul , Eftekhari-Zadeh Ehsan , Qaisi Ramy Mohammed Aiesh
Published: November 2023

Non-destructive and reliable radiation-based gauges have been routinely used in industry to determine the thickness of metal layers. When the material’s composition is understood in advance, only then can the standard radiation thickness meter be relied upon. Errors in thickness measurements are to be expected in settings where the actual composition of the material may deviate significantly from the nominal composition, such as rolled metal manufacturers. In this research, an X-ray-based system is proposed to determine the thickness of an aluminum sheet regardless of its alloy type. In the presented detection system, an X-ray tube with a voltage of 150 kV and two sodium iodide detectors, a transmission detector and a backscattering detector, were used. Between the X-ray tube and the transmission detector, an aluminum plate with different thicknesses, ranging from 2 to 45 mm, and with four alloys named 1050, 3050, 5052, and 6061 were simulated. The MCNP code was used as a very powerful platform in the implementation of radiation-based systems in this research to simulate the detection structure and the spectra recorded using the detectors. From the spectra recorded using two detectors, three features of the total count of both detectors and the maximum value of the transmission detector were extracted. These characteristics were applied to the inputs of an RBF neural network to obtain the relationship between the inputs and the thickness of the aluminum plate. The trained neural network was able to determine the thickness of the aluminum with an MRE of 2.11%. Although the presented methodology is used to determine the thickness of the aluminum plate independent of the type of alloy, it can be used to determine the thickness of other metals as well.
Collaborative Federated Learning-Based Model for Alert Correlation and Attack Scenario Recognition
Authors: Alkhpor Hadeel K. , Alserhani Faeiz M.
Published: November 2023

Planned and targeted attacks, such as the advanced persistent threat (APT), are highly sophisticated forms of attack. They involve numerous steps and are intended to remain within a system for an extended length of period before progressing to the next stage of action. Anticipating the next behaviors of attackers is a challenging and crucial task due to the stealthy nature of advanced attack scenarios, in addition to the possible high volumes of false positive alerts generated by different security tools such as intrusion detection systems (IDSs). Intelligent models that are capable of establishing a correlation individual between individual security alerts in order to reconstruct attack scenarios and to extract a holistic view of intrusion activities are required to exploit hidden links between different attack stages. Federated learning models performed in distributed settings have achieved successful and reliable implementations. Alerts from distributed security devices can be utilized in a collaborative manner based on several learning models to construct a federated model. Therefore, we propose an intelligent detection system that employs federated learning models to identify advanced attack scenarios such as APT. Features extracted from alerts are preprocessed and engineered to produce a model with high accuracy and fewer false positives. We conducted training on four machine learning models in a centralized learning; these models are XGBoost, Random Forest, CatBoost, and an ensemble learning model. To maintain privacy and ensure the integrity of the global model, the proposed model has been implemented using conventional neural network federated learning (CNN_FL) across several clients during the process of updating weights. The experimental findings indicate that ensemble learning achieved the highest accuracy of 88.15% in the context of centralized learning. CNN_FL has demonstrated an accuracy of 90.18% in detecting various attacks of APTs while maintaining a low false alarm rate.
Nattokinase: Insights into Biological Activity, Therapeutic Applications, and the Influence of Microbial Fermentation
Authors: Fang Mudannan , Yuan Beichen , Wang Meng , Liu Junfeng , Wang Zheng
Published: November 2023

Nattokinase, a serine protease that originates from the traditional food natto, has garnered widespread attention due to its pharmacological functions and therapeutic potential. This review aims to delve into the major advancements of nattokinase across various domains, particularly its emerging roles in Alzheimer’s disease prevention and the treatment of retinal diseases, thereby seeking to usher in a newfound hope in the fields of neurology and ophthalmology. However, the production and preservation of nattokinase present a multitude of challenges, including issues of unstable yield and enzyme activity loss. To address these challenges, we explore potential solutions such as the heterologous expression of the nattokinase gene, the optimization of microbial fermentation strategies, and innovative purification methods. Furthermore, we focus on enhancing the stability and protection of nattokinase through encapsulation and immobilization techniques, thus ensuring its sustainability across a wide array of applications. This review provides readers with the opportunity to gain an in-depth understanding of the diverse prospects for nattokinase applications. Future research directions will encompass a deeper exploration of its biological mechanisms, the development of novel nattokinase derivatives, and the extension of its applications into a broader spectrum of disease treatments and health maintenance.
Frass from Black Soldier Fly Larvae, Hermetia illucens, as a Possible Functional Dietary Ingredient in Channel Catfish Feed
Authors: Yıldırım-Aksoy Mediha , Eljack Rashida , Aksoy Janset , Beck Benjamin H.
Published: November 2023

Frass–the by-product of the larva meal industry–was recently shown to be a sustainable ingredient for use in diets of several fish species. Additionally, because of its possible immunomodulatory activity and anti-microbial properties, frass may have great potential as an organic method of pathogen control in aquaculture. Five diets containing 0, 5, 10, 20 and 30% frass from black soldier fly larvae, Hermetia illucens, were fed to channel catfish (5.24 ± 0.04 g) in quadruplicate aquaria to apparent satiation twice daily. At the end of the 10-week feeding trial, blood samples were collected from all groups to measure hematological and immune parameters, and to determine the effects of dietary frass on resistance to Flavobacterium covae infection. Hematological parameters (red blood cell count, hemoglobin, and hematocrit)—but not white blood cell count—were improved with the inclusion of frass. Serum glucose levels were significantly lower in fish on the diet with frass than fish on the diet without frass. Fish fed the highest dietary levels of frass (30%) had a significantly higher serum cholesterol level than fish on the control diet. Serum complement activity was significantly higher in fish on diets containing frass at levels of 10% and 20%. No significant differences were observed in other measure serum components including albumin, alkaline phosphatase, alanine aminotransferase, amylase, calcium, phosphorus, potassium, total protein, globulin, thyroxine and lysozyme activity. Even though overall mortality was low (0–17%), fish on the diets containing frass at levels 20% or more showed significantly higher survival than that of control fish or fish on lower levels of dietary frass. The use of frass in the catfish diet may prove beneficial by improving hematological parameters, and select serum immune effectors, and the overall resistance of juvenile channel catfish against F. covae infection.
Effects of Mulberry Leaf Fu Tea on the Intestines and Intestinal Flora of Goto-Kakizaki Type 2 Diabetic Rats
Authors: Liu Changwei , Zeng Hongzhe , Jiang Ronggang , Wang Kuofei , Ouyang Jian , Wen Shuai , Peng Liyuan , Xu Hao , Huang Jianan , Liu Zhonghua
Published: November 2023

Type 2 diabetes mellitus is a disease caused by hyperglycemia, an imbalance in the intestinal flora and disruption of the endocrine system. At present, it is primarily controlled through drug treatment and an improved diet. Mulberry leaf and fu brick tea were considered to have excellent hypoglycemic effects. This study used mulberry leaves and fu brick tea as raw materials to develop a dietary regulator that can assist in the prevention and alleviation of diabetes. The experiment used the Goto-Kakizaki (GK) rat model to investigate the hypoglycemic effect of mulberry leaf fu tea (MFT) and its influence on the intestinal flora of diabetic rats through methods including ELISA, tissue section observation and 16S RNA microbial sequencing. The results showed that, compared with the GK group, the intervention of mulberry leaf fu tea significantly reduced the activities of α-glucosidase (p < 0.05) and α-amylase (p < 0.05) in the duodenum of GK diabetic rats. The height of the duodenal villi was significantly reduced (p < 0.001), leading to decreased intestinal sugar absorption. At the same time, MFT alleviates the imbalance of intestinal flora caused by high blood sugar, promotes the growth of beneficial bacteria (Lactobacillus, Bifidobacterium, etc.), and inhibits the reproduction of harmful bacteria (Blautia, Klebsiella, Helicobacter, Alistipes, etc.). MFT helps reduce the secretion of toxic substances (lipopolysaccharide, p < 0.001), decreases oxidative stress and inflammation, mitigates organ damage, and improves symptoms of diabetes. Finally, the random blood glucose value of GK rats dropped from 22.79 mmol/L to 14.06 mmol/L. In summary, mulberry leaf fu tea can lower sugar absorption in diabetic rats, reduce the body’s oxidative stress and inflammatory response, regulate intestinal flora, and reduce blood sugar levels in GK rats. It is hinted that mulberry leaf fu tea could be used as a functional drink to help prevent the occurrence of diabetes.
New Technologies in Cereal Processing and Their Impact on the Physical Properties of Cereal Foods
Authors: Li Min , Niu Meng
Published: November 2023

Cereal is a general term for cereal plants or food crops, covering a wide range of foods, including rice, wheat, millet, corn and other miscellaneous grains, and representiing the most important component of the human diet [...]
Evaluation of Community Restaurants Linked to Government Food and Nutrition Safety Programs: A Scope Review
Authors: Sousa Mateus Santana , de Lira Carlos Rodrigo Nascimento , Nakano Eduardo Yoshio , Botelho Raquel Braz Assunção , de Cássia Coelho de Almeida Akutsu Rita
Published: November 2023

Community restaurants linked to government food and nutritional security programs are establishments created to offer meals to the population in socially vulnerable situations. The objective was to identify the methods, approaches, criteria, and indicators used to evaluate restaurants linked to government food and nutrition security programs. A scoping review based on the Joanna Briggs Institute’s methodology and the international guide’s recommendations of preferred reporting items for systematic reviews and meta-analyses extension for scoping reviews was conducted. Medline databases via PubMed, Lilacs, Scopus, Cochrane, Web of Science, and ScienceDirect were used. Primary observational studies, systematic reviews and meta-analyses, ethnographies, documentary studies, and case studies were included, with a quantitative, qualitative, and/or mixed approach. A total of 2498 studies were identified. After taking out 180 duplicated articles, another 2202 articles were excluded by the title. Among the 71 studies selected for complete reading, 10 did not correlate with the research objective, and 12 were included after analyzing the references, totaling 73 included studies. In this review, evaluative approaches were mapped and systematized on the menu, food consumption, food health, food security and/or insecurity, nutritional education, and human right to adequate food; users’ profile and health, implantation, history, perceptions, senses, and meanings; handlers/workers; hygienic–sanitary quality; evaluation and monitoring; physical–functional planning, and rest–intake. The presented data provide elements that can be adapted in future evaluations and describe the panorama of academic production in this area.
The Presence of the Human Auditory Ossicles—Detected Postmortem by CT Scan—As a Taphonomic Indicator
Authors: Guareschi Edda E. , Poggesi Sara , Palmesino Marco , Magni Paola A.
Published: November 2023

Introduction: Three tiny bones compose the human ossicular chain: malleus, incus and stapes. Also known as auditory ossicles, they are united by joints in the middle ear cavity of the petrous part of the temporal bone. Completely developed two years after birth, the ossicular chain is involved in the physiological process of hearing, by which sound waves from the environment are converted into electrochemical impulses. In the last 500 years, most studies have focused on the morphogenesis, morphological variability and clinical pathology of the ossicular chain, whilst only a few studies have added relevant knowledge to anthropology and forensic science. The auditory ossicles and the enclosing petrous bone are some of the hardest in the human skeleton. This is reflected in a relative resistance to fire and in the possibility of preservation and fossilization in millions of years. Materials and Methods: The literature and four present-day forensic cases were included in studying the postmortem loss of the auditory ossicles in skeletal or decomposing remains. Results indicate that it can be ascribed to their destruction or physical displacement, by either macro-micro-faunal action and/or any other natural or artificial disturbance. Discussion: Physical displacement is closely connected to the depositional environment of the skeletal remains, such as burial, entombment (sarcophagus, coffin, vault…), submersion or exposure to natural elements. Auditory ossicles can be recovered in situ, or very close to their anatomical location, when the skeletal material has been involved in an archaeological excavation. In the case of accessible or disturbed remains, scavengers may remove the tiny ossicles and/or they can slip out of the middle ear cavity following skull movements. Entombment offers effective protection against the displacement of the auditory ossicles, whereas aquatic submersion and aquatic movement almost invariably displace them. Conclusion: the preservation of the human auditory ossicles should be critically considered in the comprehensive context of any forensic investigation on human remains since it can assist the reconstruction of their taphonomic history. Taphonomic histories of remains can add crucial information to forensic investigations (e.g., the Post Mortem Interval, PMI). The aim of this study, limited by scarce relevant literature, is to discuss the potential role of the ossicular chain, detected by postmortem imaging techniques, as a taphonomical indicator in decomposing and/or skeletonized bodies.
Exploring the Reciprocal Relationship between Depressive Symptoms and Cognitive Function among Chinese Older Adults
Authors: Lu Jiehua , Ruan Yunchen
Published: November 2023

(1) Objectives: This study aims to investigate the bidirectional relationship between depressive symptoms and cognitive function among older adults in China, addressing a research gap in the context of developing nations. (2) Methods: A total of 3813 adults aged 60 and older participating in 2013, 2015, and 2018 waves of the China Health and Retirement Longitudinal Study (CHARLS) were included. A fixed-effects model and cross-lagged panel model (CLPM) was utilized. (3) Results: First, the results indicated that a significant negative correlation existed between depressive symptoms and cognitive function in older adults during the study period (β = −0.084, p < 0.001). Second, after controlling for unobserved confounding factors, the deterioration and improvement of depressive symptoms still significantly affected cognitive function (β = −0.055, p < 0.001). Third, using the cross-lagged panel model, we observed a reciprocal relationship between depressive symptoms (Dep) and cognitive function (Cog) among Chinese older adults (Dep2013 → Cog2015, β = −0.025, p < 0.01; Dep2015 → Cog2018, β = −0.028, p < 0.001; Cog2013 → Dep2015, β = −0.079, p < 0.01; Cog2015 → Dep2018, β = −0.085, p < 0.01). (4) Discussion: The reciprocal relationship between depressive symptoms and cognitive functioning in older adults emphasizes the need for integrated public health policies and clinical interventions, to develop comprehensive intervention strategies that simultaneously address depressive symptoms and cognitive decline.
Phytochemistry, Antioxidant Potential, and Antibacterial Activities of Anacyclus pyrethrum: Promising Bioactive Compounds
Authors: Baslam Abdelmounaim , Aitbaba Abdelfatah , Aboufatima Rachida , Agouram Fatimazahra , Boussaa Samia , Chait Abderrahman , Baslam Marouane
Published: November 2023

Secondary metabolites derived from plants have demonstrated significant biological activity and hold both edible and medicinal significance. These compounds play a crucial role in the development of pharmaceuticals, particularly in the context of inflammatory disorders and chronic diseases associated with oxidative stress. The objective of this study was to investigate the chemical characterization, antioxidant potential, and antibacterial properties of the aqueous extract of Anacyclus pyrethrum (AEAP). To achieve this, we employed various analytical techniques including HPLC–ESI–MS/MS, Fourier transform infrared, X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The antioxidant activity of the AEAP was assessed using DPPH (2,2-Diphenyl-1-picrylhydrazyl) and reducing power assays, while antibacterial activity was evaluated against both Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecium) using the broth microdilution assay. Our findings demonstrated significant antioxidant activity of the AEAP, as well as broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria. HPLC analysis identified important bioactive compounds, notably pellitorine and 3,4-dihydroxybenzoic acid, known for their antioxidant and antibacterial properties. Overall, the AEAP demonstrated potent antioxidant and antibacterial activities, suggesting its potential as a valuable natural source of bioactive compounds with various therapeutic applications.
Oral Furosemide and Hydrochlorothiazide/Amiloride versus Intravenous Furosemide for the Treatment of Resistant Nephrotic Syndrome
Authors: Frățilă Georgiana , Sorohan Bogdan , Achim Camelia , Andronesi Andreea , Obrișcă Bogdan , Lupușoru Gabriela , Zilișteanu Diana , Jurubiță Roxana , Bobeică Raluca , Bălănică Sonia , Micu Georgia , Mocanu Valentin , Ismail Gener
Published: November 2023

Background: Data on diuretic treatment in nephrotic syndrome (NS) are scarce. Our goal was to assess the non-inferiority of the combined oral diuretics (furosemide/hydrochlorothiazide/amiloride) compared to intravenous (i.v.) furosemide in patients with NS and resistant edema. Methods: We conducted a prospective randomized trial on 22 patients with resistant nephrotic edema (RNE), defined as hypervolemia and a FENa < 0.2%. Based on a computer-generated 1:1 randomization, we assigned patients to receive either intravenous furosemide (40 mg bolus and then continuous administration of 5 mg/h) or oral furosemide (40 mg/day) and hydrochlorothiazide/amiloride (50/5 mg/day) for a period of 5 days. Clinical and laboratory measurements were performed daily. Hydration status was assessed by bioimpedance on day 1 and at the end of day 5 after treatment initiation. The primary endpoint was weight change from baseline to day 5. Secondary endpoints were hydration status change measured by bioimpedance and safety outcomes (low blood pressure, severe electrolyte disturbances, acute kidney injury and worsening hypervolemia). Results: Primary endpoint analysis showed that after 5 days of treatment, there was a significant difference in weight change from baseline between groups [adjusted mean difference: −3.33 kg (95% CI: −6.34 to −0.31), p = 0.03], with a higher mean weight change in the oral diuretic treatment group [−7.10 kg (95% CI: −18.30 to −4.30) vs. −4.55 kg (95%CI: −6.73 to −2.36)]. Secondary endpoint analysis showed that there was no significant difference between groups regarding hydration status change [adjusted mean difference: −0.05 L (95% CI: −2.6 to 2.6), p = 0.96], with a mean hydration status change in the oral diuretic treatment group of −4.71 L (95% CI: −6.87 to −2.54) and −3.91 L (95% CI: −5.69 to −2.13) in the i.v. diuretic treatment group. We observed a significant decrease in adjusted mean serum sodium of −2.15 mmol/L [(95% CI: −4.25 to −0.05), p = 0.04]), favored by the combined oral diuretic treatment [−2.70 mmol/L (95% CI: −4.89 to −0.50) vs. −0.10 mmol/L (95%CI: −1.30 to 1.10)]. No statistically significant difference was observed between the two groups in terms of adverse events. Conclusions: A combination of oral diuretics based on furosemide, amiloride and hydrochlorothiazide is non-inferior to i.v. furosemide in weight control of patients with RNE and a similar safety profile.
Effect of Denosumab on Bone Density in Postmenopausal Osteoporosis: A Comparison with and without Calcium Supplementation in Patients on Standard Diets in Korea
Authors: Jeong Chaiho , Kim Jinyoung , Lee Jeongmin , Lim Yejee , Lim Dong-Jun , Baek Ki-Hyun , Ha Jeonghoon
Published: November 2023

The side effects and safety issues tied to calcium supplementation raise questions about its necessity in osteoporosis treatment. We retrospectively evaluated 189 postmenopausal osteoporosis patients treated with denosumab for 12 months. Patients exhibited neither renal dysfunction nor compromised general dietary intake. Patients were divided into three groups as follows: group A, weekly vitamin D 7000 IU; group B, daily vitamin D 1000 IU with elemental calcium 100 mg; and group C, daily vitamin D 1000 IU with elemental calcium 500 mg. All groups showed significant increases in bone density: +6.4 ± 4.7% for the lumbar spine, +2.2 ± 3.5% for the femoral neck, and +2.4 ± 3.8% for the total hip in group A; +7.0 ± 10.9% for the lumbar spine, +2.3 ± 5.2% for the femoral neck, and +2.4 ± 3.8% for the total hip in group B; and + 6.7 ± 8.7% for the lumbar spine, +2.5 ± 8.4% for the femoral neck, and +2.3 ± 4.0% for the total hip in group C. Serum calcium levels increased over time in all three groups with no significant difference. Changes in CTX and P1NP levels did not differ between the groups (all p > 0.05). With regular dietary intake, calcium supplementation levels showed no significant effect on bone density, bone marker changes, or hypocalcemia incidence during denosumab treatment.
Magnetic Resonance Imaging Features and Prognostic Indicators of Local Recurrence after Curettage and Cementation of Atypical Cartilaginous Tumour in the Appendicular Skeleton
Authors: Ardakani Amir Gahanbani , Morgan Rebecca , Matheron George , Havard Helard , Khoo Michael , Saifuddin Asif , Gikas Panagiotis
Published: November 2023

Objective: The aim of this study is to determine MRI features that may be prognostic indicators of local recurrence (LR) in patients treated with curettage and cementation of atypical cartilaginous tumours (ACTs) in the appendicular skeleton. Materials and Methods: This study is a retrospective review of adult patients with histologically confirmed appendicular ACT. The data collected included age, sex, skeletal location and histology from curettage, the presence of LR and oncological outcomes. The pre-operative MRI characteristics of the ACT reviewed by a specialist MSK radiologist included lesion location, lesion length, degree of medullary filling, bone expansion, cortical status and the presence of soft tissue extension. Results: A total of 43 patients were included, including 9 males and 34 females with a mean age of 42.8 years (range: 25–76 years). Tumours were located in the femur (n = 19), humerus (n = 15), tibia (n = 5), fibula (n = 2) and radius and ulna (n = 1 each). A total of 19 lesions were located in the diaphysis, 12 in the metadiaphysis, 6 in the metaphysis and 6 in the epiphysis. The mean tumour length was 61.0 mm (range: 12–134 mm). The mean follow up was 97.7 months (range: 20–157 months), during which 10 (23.3%) patients developed LR, 7 (70%) of which were asymptomatic and 3 (30%) of which presented with pain. Four patients required repeat surgery with no associated death or evidence of metastatic disease. LR was significantly commoner with tumours arising in the epiphysis or metadiaphysis, but no MRI features were predictive of LR. Conclusions: No relationship was found between the apparent ‘aggressiveness’ of an ACT of the appendicular skeleton on MRI and the development of LR following treatment with curettage and cementation.