bluuebunny/crossref_metadata_2025 huggingface dataset:
Acetic Acid Mediated for One-Pot Synthesis of Novel Pyrazolyl s-Triazine Derivatives for the Targeted Therapy of Triple-Negative Breast Tumor Cells (MDA-MB-231) via EGFR/PI3K/AKT/mTOR Signaling Cascades
Authors: Shawish Ihab , Barakat Assem , Aldalbahi Ali , Alshaer Walhan , Daoud Fadwa , Alqudah Dana A. , Al Zoubi Mazhar , Hatmal Ma’mon M. , Nafie Mohamed S. , Haukka Matti , Sharma Anamika , de la Torre Beatriz G. , Albericio Fernando , El-Faham Ayman
Published: July 2022

Here, we described the synthesis of novel pyrazole-s-triazine derivatives via an easy one-pot procedure for the reaction of β-dicarbonyl compounds (ethylacetoacetate, 5,5-dimethyl-1,3-cyclohexadione or 1,3-cyclohexadionone) with N,N-dimethylformamide dimethylacetal, followed by addition of 2-hydrazinyl-4,6-disubstituted-s-triazine either in ethanol-acetic acid or neat acetic acid to afford a novel pyrazole and pyrazole-fused cycloalkanone systems. The synthetic protocol proved to be efficient, with a shorter reaction time and high chemical yield with broad substrates. The new pyrazolyl-s-triazine derivatives were tested against the following cell lines: MCF-7 (breast cancer); MDA-MB-231 (triple-negative breast cancer); U-87 MG (glioblastoma); A549 (non-small cell lung cancer); PANC-1 (pancreatic cancer); and human dermal fibroblasts (HDFs). The cell viability assay revealed that most of the s-triazine compounds induced cytotoxicity in all the cell lines tested. However, compounds 7d, 7f and 7c, which all have a piperidine or morpholine moiety with one aniline ring or two aniline rings in their structures, were the most effective. Compounds 7f and 7d showed potent EGFR inhibitory activity with IC50 values of 59.24 and 70.3 nM, respectively, compared to Tamoxifen (IC50 value of 69.1 nM). Compound 7c exhibited moderate activity, with IC50 values of 81.6 nM. Interestingly, hybrids 7d and 7f exerted remarkable PI3K/AKT/mTOR inhibitory activity with 0.66/0.82/0.80 and 0.35/0.56/0.66-fold, respectively, by inhibiting their concentrations to 4.39, 37.3, and 69.3 ng/mL in the 7d-treatment, and to 2.39, 25.34 and 57.6 ng/mL in the 7f-treatment compared to the untreated control.
Comparison of Nozzle-Based and Nozzle-Free Electrospinning for Preparation of Fast-Dissolving Nanofibers Loaded with Ciprofloxacin
Authors: Uhljar Luca Éva , Alshweiat Areen , Katona Gábor , Chung Michael , Radacsi Norbert , Kókai Dávid , Burián Katalin , Ambrus Rita
Published: July 2022

The study aimed to prepare ciprofloxacin-loaded polyvinylpyrrolidone electrospun nanofibers for oral drug delivery, using a conventional nozzle-based and a lab-built nozzle-free electrospinning equipment. To produce nanofibers, electrospinning is the process most often used. However, from the industry’s point of view, conventional electrospinning does not have sufficiently high productivity. By omitting the nozzle, productivity can be increased, and so the development of nozzle-free processes is worthwhile. In this study, a solution of ciprofloxacin and polyvinylpyrrolidone was electrospun under similar conditions, using both single-nozzle and nozzle-free methods. The two electrospinning methods were compared by investigating the morphological and physicochemical properties, homogeneity, in vitro drug release, and cytotoxicity. The stability of the nanofibers was monitored from different aspects in a 26 month stability study. The results showed that the use of the nozzle-free electrospinning was preferable due to a higher throughput, improved homogeneity, and the enhanced stability of nanofiber mats, compared to the nozzle-based method. Nevertheless, fast dissolving nanofibers loaded with poorly water-soluble ciprofloxacin were produced by both electrospinning methods. The beneficial properties of these nanofibers can be exploited in innovative drug development; e.g., nanofibers can be formulated into orodispersible films or per os tablets.
An Overview on Immunogenic Cell Death in Cancer Biology and Therapy
Authors: Rodrigues Mosar Corrêa , Morais José Athayde Vasconcelos , Ganassin Rayane , Oliveira Giulia Rosa Tavares , Costa Fabiana Chagas , Morais Amanda Alencar Cabral , Silveira Ariane Pandolfo , Silva Victor Carlos Mello , Longo João Paulo Figueiró , Muehlmann Luis Alexandre
Published: July 2022

Immunogenic cell death (ICD) is a modality of regulated cell death that is sufficient to promote an adaptive immune response against antigens of the dying cell in an immunocompetent host. An important characteristic of ICD is the release and exposure of damage-associated molecular patterns, which are potent endogenous immune adjuvants. As the induction of ICD can be achieved with conventional cytotoxic agents, it represents a potential approach for the immunotherapy of cancer. Here, different aspects of ICD in cancer biology and treatment are reviewed.
Discordance in Addressing Opioid Crisis in Rural Communities: Patient and Provider Perspectives
Authors: Qudah Bonyan , Maurer Martha A. , Mott David A. , Chui Michelle A.
Published: July 2022

Providing patient-centered care to manage chronic pain and opioid use disorder (OUD) is associated with improved health outcomes. However, adopting a holistic approach to providing care is often challenging in rural communities. This study aims to identify and contrast challenges to providing patient-centered care from the perspective of patients and providers. A participatory design approach was adopted to elicit the perceptions of providers and patients with lived experiences of chronic pain and/or OUD in Jefferson County, Wisconsin. Two focus groups were conducted with each stakeholder group to identify problems that participants face with respect to chronic pain management and OUD and possible solutions. Four interviews were conducted with providers experienced in chronic pain management. Analysis of focus group sessions and interviews show consensus among patients and providers that lack of behavioral health and recovery resources create barriers to effectively manage OUD and chronic pain. However, there was discordance among the two groups about other barriers such as patient and provider attitudes, tapering approach, and access to medications for OUD. This tension among patients and providers can influence patients’ retention in therapy. More efforts are needed to mitigate stigma among providers in rural communities and support psychosocial needs of patients.
Stabilized Time Transfer via a 1000-km Optical Fiber Link Using High-Precision Delay Compensation System
Authors: Liu Bo , Guo Xinxing , Kong Weicheng , Liu Tao , Dong Ruifang , Zhang Shougang
Published: July 2022

Variations in optical fiber length and refractive index are induced by environmental perturbation, resulting in an additional dynamic propagation delay in fiber-based time synchronization systems, which deteriorate their transfer stability. This disadvantage can be significantly reduced by transmitting the time signal in both directions through fiber and constructing a feedback loop to compensate the propagation delay at the remote end of the link. This paper proposes an analog-digital hybrid proportional integral derivative (PID) control compensation system based on the time-frequency phase-locked loop (TF-PLL). The system is designed to keep the merits of wide servo bandwidth, servo accuracy, and a large dynamic delay compensation range up to 1 s, which is much greater than that reported in previous studies. For proving the validity of this proposed scheme, a self-developed optical fiber time synchronization equipment based on the delay compensation system is applied. The delay compensation system is used on a 1100-km long laboratory optical fiber, and the results show that the time synchronization stability in terms of time deviation (TDEV) is less than 5.92 ps/1 s and 2.56 ps/10,000 s. After successful laboratory evaluation, the proposed system is installed on a real 988.48-km line between the Xi’an Lintong branch of the National Time Service Center (NTSC) and Linfen City, Shanxi Province, realizing the time synchronization of 10 stations along the optical fiber link. The experimental results in the 988.48-km link illustrate that the measured time difference with a peak-to-peak value of 176 ps, the standard deviation of 19.3 ps, and a TDEV of less than 10.49 ps/1 s and 2.31 ps/40,000 s is achieved. The high-precision time delay compensation system proposed in this paper is simple, reliable, and accurate; has a wide range of compensation; and opens up a feasible scheme for providing synchronized time signals to multiple users over the long-distance field optical fiber networks.
Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins
Authors: Malarz Janusz , Michalska Klaudia , Yudina Yulia V. , Stojakowska Anna
Published: July 2022

Due to their chemical properties and biological activity, antioxidants of plant origin have gained interest as valuable components of the human diet, potential food preservatives and additives, ingredients of cosmetics and factors implicated in tolerance mechanisms against environmental stress. Plant polyphenols are the most prominent and extensively studied, albeit not only group of, secondary plant (specialized) metabolites manifesting antioxidative activity. Because of their potential economic importance, the productive and renewable sources of the compounds are desirable. Over thirty years of research on hairy root cultures, as both producers of secondary plant metabolites and experimental systems to investigate plant biosynthetic pathways, brought about several spectacular achievements. The present review focuses on the Rhizobium rhizogenes-transformed roots that either may be efficient sources of plant-derived antioxidants or were used to elucidate some regulatory mechanisms responsible for the enhanced accumulation of antioxidants in plant tissues.
Enhanced Apiaceous Potyvirus Phylogeny, Novel Viruses, and New Country and Host Records from Sequencing Apiaceae Samples
Authors: Fox Adrian , Gibbs Adrian J. , Fowkes Aimee R. , Pufal Hollie , McGreig Sam , Jones Roger A. C. , Boonham Neil , Adams Ian P.
Published: July 2022

The family Apiaceae comprises approximately 3700 species of herbaceous plants, including important crops, aromatic herbs and field weeds. Here we report a study of 10 preserved historical or recent virus samples of apiaceous plants collected in the United Kingdom (UK) import interceptions from the Mediterranean region (Egypt, Israel and Cyprus) or during surveys of Australian apiaceous crops. Seven complete new genomic sequences and one partial sequence, of the apiaceous potyviruses apium virus Y (ApVY), carrot thin leaf virus (CaTLV), carrot virus Y (CarVY) and celery mosaic virus (CeMV) were obtained. When these 7 and 16 earlier complete non-recombinant apiaceous potyvirus sequences were subjected to phylogenetic analyses, they split into 2 separate lineages: 1 containing ApVY, CeMV, CarVY and panax virus Y and the other CaTLV, ashitabi mosaic virus and konjac virus Y. Preliminary dating analysis suggested the CarVY population first diverged from CeMV and ApVY in the 17th century and CeMV from ApVY in the 18th century. They also showed the “time to most recent common ancestor” of the sampled populations to be more recent: 1997 CE, 1983 CE and 1958 CE for CarVY, CeMV and ApVY, respectively. In addition, we found a new family record for beet western yellows virus in coriander from Cyprus; a new country record for carrot torradovirus-1 and a tentative novel member of genus Ophiovirus as a co-infection in a carrot sample from Australia; and a novel member of the genus Umbravirus recovered from a sample of herb parsley from Israel.
Automated Real-Time Identification of Medicinal Plants Species in Natural Environment Using Deep Learning Models—A Case Study from Borneo Region
Authors: Malik Owais A. , Ismail Nazrul , Hussein Burhan R. , Yahya Umar
Published: July 2022

The identification of plant species is fundamental for the effective study and management of biodiversity. In a manual identification process, different characteristics of plants are measured as identification keys which are examined sequentially and adaptively to identify plant species. However, the manual process is laborious and time-consuming. Recently, technological development has called for more efficient methods to meet species’ identification requirements, such as developing digital-image-processing and pattern-recognition techniques. Despite several existing studies, there are still challenges in automating the identification of plant species accurately. This study proposed designing and developing an automated real-time plant species identification system of medicinal plants found across the Borneo region. The system is composed of a computer vision system that is used for training and testing a deep learning model, a knowledge base that acts as a dynamic database for storing plant images, together with auxiliary data, and a front-end mobile application as a user interface to the identification and feedback system. For the plant species identification task, an EfficientNet-B1-based deep learning model was adapted and trained/tested on a combined public and private plant species dataset. The proposed model achieved 87% and 84% Top-1 accuracies on a test set for the private and public datasets, respectively, which is more than a 10% accuracy improvement compared to the baseline model. During real-time system testing on the actual samples, using our mobile application, the accuracy slightly dropped to 78.5% (Top-1) and 82.6% (Top-5), which may be related to training data and testing conditions variability. A unique feature of the study is the provision of crowdsourcing feedback and geo-mapping of the species in the Borneo region, with the help of the mobile application. Nevertheless, the proposed system showed a promising direction toward real-time plant species identification system.
Gene Expression, Histology and Histochemistry in the Interaction between Musa sp. and Pseudocercospora fijiensis
Authors: Soares Julianna Matos da Silva , Rocha Anelita de Jesus , Nascimento Fernanda dos Santos , Amorim Vanusia Batista Oliveira de , Ramos Andresa Priscila de Souza , Ferreira Cláudia Fortes , Haddad Fernando , Amorim Edson Perito
Published: July 2022

Bananas are the main fruits responsible for feeding more than 500 million people in tropical and subtropical countries. Black Sigatoka, caused by the fungus Pseudocercospora fijiensis, is one of the most destructive disease for the crop. This fungus is mainly controlled with the use of fungicides; however, in addition to being harmful to human health, they are associated with a high cost. The development of resistant cultivars through crosses of susceptible commercial cultivars is one of the main focuses of banana breeding programs worldwide. Thus, the objective of the present study was to investigate the interaction between Musa sp. and P. fijiensis through the relative expression of candidate genes involved in the defence response to black Sigatoka in four contrasting genotypes (resistant: Calcutta 4 and Krasan Saichon; susceptible: Grand Naine and Akondro Mainty) using quantitative real-time PCR (RT–qPCR) in addition to histological and histochemical analyses to verify the defence mechanisms activated during the interaction. Differentially expressed genes (DEGs) related to the jasmonic acid and ethylene signalling pathway, GDSL-like lipases and pathogenesis-related proteins (PR-4), were identified. The number and distance between stomata were directly related to the resistance/susceptibility of each genotype. Histochemical tests showed the production of phenolic compounds and callosis as defence mechanisms activated by the resistant genotypes during the interaction process. Scanning electron microscopy (SEM) showed pathogenic structures on the leaf surface in addition to calcium oxalate crystals. The resistant genotype Krasan Saichon stood out in the analyses and has potential for use in breeding programs for resistance to black Sigatoka in banana and plantains.
Chemical Constituents from the Aerial Parts of Artemisia iwayomogi and Their Anti-Neuroinflammatory Activities
Authors: Son So-Ri , Ju In Gyong , Kim Jinhee , Park Keon-Tae , Oh Myung Sook , Jang Dae Sik
Published: July 2022

Neuroinflammation, predominantly mediated by microglial activation, is a key immunological response in the pathogenesis of neurodegenerative disorders. In our preliminary study, the aerial part of Artemisia iwayomogi inhibits LPS-induced microglial activation. The present study aims to identify chemical constituents with anti-neuroinflammatory properties in the aerial parts of A. iwayomogi. Two new guaianolide sesquiterpenes, iwayomogins A and B (1 and 2), along with thirteen known sesquiterpene lactones (3–15), one diterpene glycoside (16), and nine phenolic compounds (17–25) were isolated from the aerial parts of A. iwayomogi by repeated chromatography. The structures of the isolates were elucidated by their spectroscopic data. All isolates were evaluated for their inhibitory activities on nitric oxide (NO) production in LPS-induced BV-2 microglial cells. 2,3-Dehydro-1-epi-asperilin (11) exhibited the strongest inhibitory effect on NO production (IC50 value of 1.78 μM). In the molecular docking study, three compounds (1, 2, and 11) showed good binding affinities with iNOS. Additionally, compounds 1, 2, and 11 inhibit pro-inflammatory cytokines (TNF-α and IL-6) in dose-dependent manners. The present study demonstrates that the chemical constituents from A. iwayomogi inhibit NO production and pro-inflammatory cytokine release in BV-2 cells. However, further evaluation with biological experiments utilizing in vivo models is necessary.
Influence of Schumann Range Electromagnetic Fields on Components of Plant Redox Metabolism in Wheat and Peas
Authors: Mshenskaya Natalia , Sinitsyna Yulia , Kalyasova Ekaterina , Valeria Koshcheeva , Zhirova Anastasia , Karpeeva Irina , Ilin Nikolay
Published: July 2022

The Schumann Resonances (ScR) are Extremely Low Frequency (ELF) electromagnetic resonances in the Earth-ionosphere cavity excited by global lightning discharges. ScR are the part of electromagnetic field (EMF) of Earth. The influence of ScR on biological systems is still insufficiently understood. The purpose of the study is to characterize the possible role of the plant cell redox metabolism regulating system in the Schumann Resonances EMF perception. Activity of catalase and superoxide dismutase, their isoenzyme structure, content of malondialdehyde, composition of polar lipids in leaf extracts of wheat and pea plants treated with short-time (30 min) and long-time (18 days) ELF EMF with a frequency of 7.8 Hz, 14.3 Hz, 20.8 Hz have been investigated. Short-time exposure ELF EMF caused more pronounced bio effects than long-time exposure. Wheat catalase turned out to be the most sensitive parameter to magnetic fields. It is assumed that the change in the activity of wheat catalase after a short-term ELF EMF may be associated with the ability of this enzyme to perceive the action of a weak EMF through calcium calmodulin and/or cryptochromic signaling systems.
The Rise and Fall of Billionaire siRNAs during Reproductive Development in Rice
Authors: Wang Lili , Xu Dachao , Zeng Longjun , Yang Dong-Lei
Published: July 2022

RNA polymerase IV-dependent siRNAs, usually 24 nt in length, function in the RNA-directed DNA methylation that is responsible for de novo methylation in plants. We analyzed 24 nt siRNAs in inflorescences and found that among the 20,200 24 nt siRNA clusters, the top 0.81% highly expressed clusters accounted for more than 68% of the 24 nt siRNA reads in inflorescences. We named the highly expressed siRNAs as billionaire siRNAs (bill-siRNAs) and the less-expressed siRNAs as pauper siRNAs (pau-siRNAs). The bill-siRNAs in inflorescences are mainly derived from the ovary. Female gametes produced more bill-siRNAs than male gametes. In embryos and seedlings developed from fertilized egg cells, the bill-siRNAs from gametes disappeared. The endosperm, which develops from the fertilized central cell, also contained no bill-siRNAs from gametes but did contain newly and highly expressed siRNAs produced in different regions. In contrast, bill-siRNAs from the ovaries were maintained in the seed coat. The biosynthesis of bill-siRNAs in various tissues and cells is dependent on OsRDR2 (RNA-dependent RNA polymerase 2) and Pol IV (DNA-dependent RNA polymerase IV). Similar to the pau-siRNAs, the first base of bill-siRNAs is enriched at adenine, and bill-siRNAs can direct DNA methylation in various tissues.
Photodegradation of Polycyclic Aromatic Hydrocarbons from Coal Tar into Mine Wastewaters and Sewage Wastewater on a Flat-Bed Photoreactor
Authors: Batchamen Mougnol Jean Bedel , Waanders Frans , Fosso-Kankeu Elvis , Al Alili Ali R.
Published: July 2022

Wastewater treatment has been widely focused on the undesirable pollutants derived from various activities such as coking, coal gasification, oil spills, and petroleum. These activities tend to release organic pollutants, however polycyclic aromatic hydrocarbons (PAHs) happen to be highlighted as the most carcinogenic pollutant that easily comes into contact with the environment and humans. It causes major challenges due to its lingering in the environment and chemical properties. Although various techniques such as ions exchange, advanced oxidation, and reverse osmosis have been conducted, some of them have been ignored due to their cost-effectiveness and ability to produce a by-product. Therefore, there is a need to develop and implement an effective technique that will alleviate the organic pollutants (PAHs) in various water sources. In this study, a self-made flat-bed photoreactor was introduced to degrade PAHs in various water sources such as acidic mine drainage, alkaline mine drainage, and sewage wastewater. A previous study was conducted, and only 7.074 mg/L, 0.3152 mg/L and 1.069 mg/L in 4 weeks and thereafter 19.255 mg/L, 1.615 mg/L and 1.813 mg/L in 8 weeks in acidic mine drainage, alkaline mined, drainage, and sewage wastewater leachate from a 2916.47 mg/L of PAHs in coal tar, was analysed. It was found that the flat-bed photoreactor was highly effective and able to obtain a removal efficiency of 64%, 55%, and 58%, respectively; without the flat-bed photoreactor, happened the removal efficiency was of 53%, 33%, and 39%, respectively, in 60 min in acidic mine drainage, alkaline mine drainage, and sewage wastewater. The photodegradation of PAHs was favoured in the acidic mine drainage, followed by sewage wastewater and alkaline mine drainage respective, showing time and solar irradiation dependence.
A Brief Review of Poly(Vinyl Chloride) (PVC) Recycling
Authors: Lewandowski Krzysztof , Skórczewska Katarzyna
Published: July 2022

Bearing in mind the aspiration of the world economy to create as complete a closed loop of raw materials and energy as possible, it is important to know the individual links in such a system and to systematise the knowledge. Polymer materials, especially poly(vinyl chloride) (PVC), are considered harmful to the environment by a large part of society. The work presents a literature review on mechanical and feedstock recycling. The advantages and disadvantages of various recycling methods and their development perspectives are presented. The general characteristics of PVC are also described. In conclusion, it is stated that there are currently high recycling possibilities for PVC material and that intensive work is underway on the development of feedstock recycling. Based on the literature review, it was found that PVC certainly meets the requirements for materials involved in the circular economy.
Role of Biomaterials Used for Periodontal Tissue Regeneration—A Concise Evidence-Based Review
Authors: Varghese Jothi , Rajagopal Anjale , Shanmugasundaram Shashikiran
Published: July 2022

Periodontal infections are noncommunicable chronic inflammatory diseases of multifactorial origin that can induce destruction of both soft and hard tissues of the periodontium. The standard remedial modalities for periodontal regeneration include nonsurgical followed by surgical therapy with the adjunctive use of various biomaterials to achieve restoration of the lost tissues. Lately, there has been substantial development in the field of biomaterial, which includes the sole or combined use of osseous grafts, barrier membranes, growth factors and autogenic substitutes to achieve tissue and bone regeneration. Of these, bone replacement grafts have been widely explored for their osteogenic potential with varied outcomes. Osseous grafts are derived from either human, bovine or synthetic sources. Though the biologic response from autogenic biomaterials may be better, the use of bone replacement synthetic substitutes could be practical for clinical practice. This comprehensive review focuses initially on bone graft replacement substitutes, namely ceramic-based (calcium phosphate derivatives, bioactive glass) and autologous platelet concentrates, which assist in alveolar bone regeneration. Further literature compilations emphasize the innovations of biomaterials used as bone substitutes, barrier membranes and complex scaffold fabrication techniques that can mimic the histologically vital tissues required for the regeneration of periodontal apparatus.
Graphene Oxide and Fluorescent-Aptamer-Based Novel Aptasensors for Detection of Metastatic Colorectal Cancer Cells
Authors: Chen Hang , Zhang Shurui , Hsiao Yung-Chin , Wang Qun , Yu Jau-Song , Li Wanming
Published: July 2022

Early diagnosis of metastatic colorectal cancer (mCRC) is extremely critical to improve treatment and extend survival. W3 is an aptamer that can specifically bind to mCRC cells with high affinity. Graphene oxide (GO) is a two-dimensional graphitic carbon nanomaterial, which has widely used in constructing biosensors. In this study, we have developed a no-wash fluorescent aptasensor for one-step and sensitive detection of mCRC LoVo cells. It is based on fluorescence resonance energy transfer (FRET) between GO and the W3 aptamer labeled with 5-carboxyfluorescein (FAM). GO can quench the green fluorescence of the FAM-labeled W3 (FAM-W3). In the presence of the target cells, FAM-W3 preferentially binds the target cells and detaches from the surface of GO, leading to the fluorescence of FAM recovery. It was demonstrated that the fluorescence recovery increases linearly in a wide range of 0~107 cells/mL (R2 = 0.99). The GO-based FAM-labeled W3 aptasensor (denoted as FAM-W3-GO) not only specifically recognizes mCRC cell lines (LoVo and HCT116), but also sensitively differentiates the target cells from mixed cells, even in the presence of only 5% of the target cells. Furthermore, FAM-W3-GO was applied to detect LoVo cells in human whole blood, which showed good reproducibility with an RSD range of 1.49% to 1.80%. Therefore, FAM-W3-GO may have great potential for early diagnosis of mCRC. This strategy of GO-based fluorescent aptasensor provides a simple, one-step, and highly sensitive approach for the detection of mCRC cells.
Chitosan Effect on Hardening Dynamics of Calcium Phosphate Cement: Low-Field NMR Relaxometry Investigations
Authors: Lacan Ioana , Moldovan Mărioara , Sarosi Codruța , Ardelean Ioan
Published: July 2022

Calcium phosphate cements are used in dentistry and orthopedics to repair and reconstruct bone defects. The properties of these bone cements can be improved by introducing additives into their composition. One favorable additive is chitosan, which can be beneficial but can also cause considerable damage if it has a high load, thus, limiting its clinical applicability and performance. That is why understanding chitosan’s role in cement composition is an important issue when developing new materials. The present work uses low-field nuclear magnetic resonance (NMR) relaxometry to investigate the effect introduced by the addition of chitosan on the hardening process of calcium phosphate cement. Two samples, prepared with and without chitosan, were comparatively investigated during the first six minutes of hardening. The liquid evolution inside these samples was monitored using transverse relaxation time distributions. It demonstrated an acceleration effect on the hardening dynamics introduced by the presence of chitosan. Furthermore, it was shown that even after one hour of hardening, there were still unreacted monomers inside the bone cement and their amount was reduced in the presence of chitosan.
Wearable Natural Rubber Latex Gloves with Curcumin for Torn Glove Detection in Clinical Settings
Authors: Sarih Norfatirah Muhamad , Dzulkafly Nuur Syuhada , Maher Simon , Rashid Azura A.
Published: July 2022

Glove tear or perforation is a common occurrence during various activities that require gloves to be worn, posing a significant risk to the wearer and possibly others. This is vitally important in a clinical environment and particularly during surgical procedures. When a glove perforation occurs (and is noticed), the glove must be replaced as soon as possible; however, it is not always noticeable. The present article is focused on the design and development of a novel fluorescence-based sensing mechanism, which is integrated within the glove topology, to help alert the wearer of a perforation in situ. We hypothesized that natural rubber gloves with curcumin infused would yield fluorescence when the glove is damaged, particularly when torn or punctured. The glove design is based on double-dipping between Natural Rubber Latex (NRL) and an inner layer of latex mixed with curcumin, which results in a notable bright yellow-green emission when exposed to UV light. Curcumin (Cur) is a phenolic chemical found primarily in turmeric that fluoresces yellowish-green at 525 nm. The tear region on the glove will glow, indicating the presence of a Cur coating/dipping layer beneath. NRL film is modified by dipping it in a Cur dispersion solution mixed with NRL for the second dipping layer. Using Cur as a filler in NRL also has the distinct advantage of allowing the glove to be made stronger by evenly distributing it throughout the rubber phase. Herein, the optimized design is fully characterized, including physicochemical (fluorescence emission) and mechanical (tensile and tear tests) properties, highlighting the clear potential of this novel and low-cost approach for in situ torn glove detection.
Effects of Coating Parameters of Hot Filament Chemical Vapour Deposition on Tool Wear in Micro-Drilling of High-Frequency Printed Circuit Board
Authors: Kwok Fung Ming , Sun Zhanwen , Yip Wai Sze , Kwok Kwong Yu David , To Suet
Published: July 2022

High-frequency and high-speed printed circuit boards (PCBs) are made of ceramic particles and anisotropic fibres, which are difficult to machine. In most cases, severe tool wear occurs when drilling high-frequency PCBs. To protect the substrate of the drills, diamond films are typically fabricated on the drills using hot filament chemical vapour deposition (HFCVD). This study investigates the coating characteristics of drills with respect to different HFCVD processing parameters and the coating characteristics following wear from machining high-frequency PCBs. The results show that the methane concentration, processing time and temperature all have a significant effect on the grain size and coating thickness of the diamond film. The grain size of the film obviously decreases as does the methane concentration, while the coating thickness increases. By drilling high-frequency PCBs with drills with nanocrystalline and microcrystalline grain sizes, it is discovered that drills with nanocrystalline films have a longer tool life than drills with microcrystalline films. The maximum length of the flank wear of the nanocrystalline diamond-coated drill is nearly 90% less than microcrystalline diamond-coated tools. Moreover, drills with thinner films wear at a faster rate than drills with thicker films. The findings highlight the effects of HFCVD parameters for coated drills that process high-frequency PCBs, thereby contributing to the production of high quality PCBs for industry and academia.
A Study on the Application of Discrete Wavelet Decomposition for Fault Diagnosis on a Ship Oil Purifier
Authors: Lee Songho , Lee Taehyun , Kim Jeongyeong , Lee Jongjik , Ryu Kyungha , Kim Yongjin , Park Jong-Won
Published: July 2022

With the development of the Internet of things, big data, and AI leading the 4th industrial revolution, it has become possible to acquire, manage, and analyze vast and diverse condition signals from various industrial machinery facilities. In addition, it has been revealed that various and large amounts of signals acquired from the facilities can be utilized for fault diagnosis. Currently, while data-driven fault diagnosis techniques applicable to the facilities are being developed, it has been tried to apply the techniques for the development of fully autonomous ships in the shipbuilding and shipping industry. Since the autonomous ships must be able to detect and diagnose the failures on their own in real time, the overall research is required on how to acquire signals from the ship facilities and use them to diagnose their failures. In this study, a fault diagnosis framework was proposed for condition-based maintenance (CBM) of ship oil purifiers, which are an auxiliary facility in the engine system of a ship. First, an oil purifier test-bed for simulating faults was built to obtain data on the state of the equipment. After extracting features using discrete wavelet decomposition from the data, the features were visualized by using t-distributed stochastic neighbor embedding, and were used to train support vector machine-based diagnostic models. Finally, the trained models were evaluated with Accuracy and F1 score, and some models scored 0.99 or higher, confirming high diagnostic performance. This study can be used as a reference for establishing CBM system and fault diagnosis system. Furthermore, this study is expected to improve the safety and reliability of oil purifiers in Degree 4 MASS.
A Mathematical Kinetic Model and Network Analysis for Multicomponent Dissolution Relationships during the Extraction of Natural Products
Authors: Tang Yu , Zhou Yiqun , Zhang Yutian , Deng Kaiwen , Liu Zhigang , Liu Wenlong , He Fuyuan
Published: July 2022

Traditional Chinese Medicine (TCM) has a long history and typical ethnic traits. Astragalus and Angelica are used in a natural product called a buyang huanwu decoctionand are considered to function as both food and medicine; such products are called a “homology of medicine and food”. In this study, we examined the complex extraction kinetics that occur during the preparation of the natural product BYHWD. Mathematical tools, including the Laplace transformation and Fick’s law, were used to set up kinetic equations for different components in a model of the decoction. We selected the five most important bioactive ingredients of the BYHWD to find the most important speed control component. The intensity and capacity process parameters of the model were determined. A kinetic model was used to quantitatively analyze the dissolution restriction mechanism among the major components. Further, a component–effect network relationship was established to study the interactions of different components during extraction, considering the integrative effect of TCM compositions. Finally, using network pharmacology, certain network parameters were determined through topological analysis. The results indicate that Astragaloside IV exerts the strongest control over the dissolution rates of other components. The BYHWD has a short average path and a high clustering coefficient. The theoretical and experimental results can be used to quantitatively simulate and optimize TCM extraction processes.
Influence of Road Types on Transiting Test for Aerodynamic Coefficient Measurements Using Passive Wind Generated by a Moving Vehicle
Authors: Zhang Xiaoli , Hou Linjie , Hao Yanbo , Zheng Xufen , Wan Ru , Ren Duochang , Li Shengli
Published: July 2022

A transiting test using vehicle-driving passive wind is a novel experimental technique to perform aerodynamic tests, proposed by the authors. However, this low-cost, short-cycle, and symmetrical test method can be influenced by many factors. In this paper, a comparative study on the influence of road types on a transiting test of aerodynamic coefficient measurements is presented. The tests were carried out on three different roads (expressway, viaduct highway, and tunnel highway); for each road type, structure models with and without end plates were investigated. For all the road types, measured turbulence intensity (around 4%) was similar to that of the wind tunnel test under the following conditions: 3 °C temperature, 42% humidity, 0.28 m/s natural wind speed, vehicle driving in a straight line with stable speed, and no traffic flow. All the tests were conducted under the above conditions. To deal with the interference components in the original data signals obtained from a viaduct highway and tunnel highway, a low-pass filter with a 1 Hz cutoff frequency was used. With wind attack angles changing, the tendency of the aerodynamic coefficients of the test model in the transiting test showed satisfactory consistency with that of the wind tunnel test; and the results exhibited fine repeatability. Regarding the drag coefficients, the test model with end plates was in good agreement with those from the wind tunnel test. Compared with the wind tunnel test, the results of expressway showed the best consistency, considering turbulence intensity, pavement surface roughness, and other factors. Thus, the transiting test in future research should be conducted on the expressway, with end plates installed on both ends of the structure model.
Analytical Solutions Formulated in the Time Domain for Three-Dimensional Heat Diffusion Equation
Authors: Cruz Rojas Alan
Published: July 2022

Two different strategies are provided to generate solutions to the three-dimensional heat diffusion equation. The first strategy is inspired by the well-known one-dimensional heat polynomial, which consists of an infinite set of polynomials, which are solutions to the one-dimensional heat diffusion equation. The second strategy is based on an exponential type function. None of the solutions presented here can be obtained by the method of separation of variables. The mathematical developments proving that, indeed, the particular solutions generated with both strategies satisfy the three-dimensional heat diffusion equation are presented. The analytical solutions are validated by generating the corresponding numerical solutions with the method of finite differences. When comparing both analytical and numerical solutions, it is found that they are identical. In addition, as part of the results, it is found that there are exponential solutions that reproduce the behavior of polynomial solutions. Finally, an example of the use of heat polynomials in engineering applications is provided.
Developing an Anomaly Detection System for Automatic Defective Products’ Inspection
Authors: Hung Yu-Hsin
Published: July 2022

Since unqualified products cause enterprise revenue losses, product inspection is essential for maintaining manufacturing quality. An automated optical inspection (AOI) system is an efficient tool for product inspection, providing a convenient interface for users to view their products of interest. Specifically, in the screw manufacturing industry, the conventional methods are the human visual inspection of the product and for the inspector to view the product image displayed on the dashboard of the AOI system. However, despite the inspector and the approach used, inspection results strongly depend on the inspector’s experience. Moreover, machine learning algorithms could improve the efficiency of human visual inspection, thus addressing the above problem. Based on these facts, we improved anomaly detection efficiency during product inspection, using product image data from the AOI system to obtain valuable information. This study notably used the visual geometry group network, Inception V3, and Xception algorithms to detect qualified and unqualified products during product image analytics. Therefore, we considered that the analyzed results could be integrated into a proposed cloud system for human–machine interaction. Thus, administrators can receive reminders concerning the anomaly-inspected notification through the proposed cloud system, comprising a message queuing telemetry transport protocol, an application programming interface, and a cloud dashboard. From the experimental results, the above-mentioned algorithms had more than 93% accuracy, especially Xception, which had a better performance during the defective type classification. From our study, the proposed system can successfully apply the obtained data in data communication, anomaly dashboards, and anomaly notifications.
A State-of-the-Art Review on the Incorporation of Recycled Concrete Aggregates in Geopolymer Concrete
Authors: Nikmehr Bahareh , Al-Ameri Riyadh
Published: July 2022

Geopolymer concrete (GC) has the potential to incorporate recycled concrete aggregates (RCA) obtained from construction and demolition waste. This research aims to review the current state-of-the-art knowledge of the RCA in GC and identify the existing knowledge gaps for future research direction. This paper highlights the essential factors that impact the GC’s mechanical and durability properties. Moreover, the influence of various percentages of coarse and fine RCA and the pattern of their replacement will be assessed. The effect of aluminosilicate material, alkaline activators, and curing regime also will be evaluated. Besides, the durability-related characteristics of this concrete will be analysed. The impact of exposure to a higher temperature, freeze–thaw cycles, marine environment, and acid and alkali attack will be comprehensively reviewed. A literature review revealed that increasing alumina silicate content, such as slag and metakaolin, and increasing the Na2SiO3/NaOH ratio and alkali-activator-to-binder ratio improve the hardened GC. However, increasing slag and metakaolin content and the Na2SiO3/NaOH ratio has an adverse impact on its workability. Therefore, finding the optimum mix design for using RCA in GC is vital. Moreover, there is a scope for developing a self-compacting GC cured at ambient temperature using RCA.
Spatiality and Symbolic Characteristics: Dynamics of Collective Religious Rituals among Sunni Muslims and Russian Orthodox Christians in Kazakhstan during the COVID-19 Pandemic
Authors: Yerekesheva Laura
Published: July 2022

The article’s purpose is to discuss on a cross-disciplinary plane whether the space’s changing dimension (in terms of social distancing), caused by the COVID-19 pandemic, could affect religious symbols’ characteristics and rituals, leading to new symbolical representation. This is analyzed by addressing the influence of the pandemic on conducting religious practices (Friday Prayer, Namaz, Ramadan, Qurban-ait (Eid al Adha), Sunday Sermon, Easter) among the Sunni Muslims and Russian Orthodox Church believers in Kazakhstan. Theoretically, it seeks to correlate inner dynamics of the typology, or ‘production of space’ vis-à-vis symbolic characteristics and elements of ritual. The proposed hypothesis is that (1) if there is a direct correlation between spatiality and symbols (that are ‘deployed’ in rituals), (2) then change of spatiality can affect the performance of symbol’s each characteristic, and the rituals. The following research methods have been used: online group interview in the religious focus groups differed in religious makeup (Sunni Islamic and Russian Orthodox Church) and thematic analysis. The empirical findings support the hypothesis and show that social distancing triggered the fluctuation and changes pertaining to the figurative quality and perceptibility of the symbol, following the modification of religious rituals and the appearance of a wide range of perceptions, respectively. Though the full-fledged symbolization of the changes of religious rituals due to the pandemic is not the case in today’s Kazakhstan, some elements of new evolving attitude are evident.
Formation of Lay Catholics: Franciscan Inspirations
Authors: Fiałkowski Marek
Published: July 2022

Currently, the formation of lay Catholics is one of the key tasks of the Church. The Synod of Bishops, Towards a Synodal Church: Communion, Participation, and Mission, convened by Pope Francis, served as a reminder of this. In its new format, i.e., phased consultations and meetings, the Synod calls for the involvement of lay Catholics in listening to one another and recognising directions for the Church renewal. This emphasises the need for an ongoing effort to form the faithful. There are many suggestions in the Church for the formation of lay Catholics. Franciscan spirituality, which continues to inspire and attract people, is one of these suggestions. In his teaching, the current pope likes to refer to St. Francis of Assisi, drawing from his writings and example of life. This work aimed to present selected elements of Franciscan spirituality that seem useful in the formation of lay Catholics for their service in the Church and the world. Six elements that can be drawn from rich Franciscan spirituality were analysed and they seem relevant for today’s Church: fidelity to the Church, openness to the world and rejection of its evil, apostolate “in via”, promotion of the laity, poverty at the service of the Gospel, and openness to the people rejected by society. The discussion of these elements is preceded by a synthetic presentation of the nature and purpose of the formation of lay Catholics.
Korean Potalaka: Legends about Naksan Temple Examined through Mountain and Sea Worship
Authors: Vörös Erika Erzsébet
Published: July 2022

Several sites in East Asia have been identified as Potalaka, originally thought to be located near the southern seas of India. The basis of this phenomenon is built upon the nature of Avalokiteśvara as a mediator between sentient beings and buddhas, the nature of Potalaka as a boundary between their worlds, and Buddhist philosophy. The belief in the abode of Avalokiteśvara bodhisattva on Earth incorporates various places into a Buddhist world transcending borders. This paper examines Korean beliefs about Potalaka and Avalokiteśvara through legends about Naksan Temple, with special emphasis on their relationship with mountain and sea worship. At the same time, the study attempts to connect the beliefs with the philosophical background of Hwaŏm tradition, which is in close relation with this ritual site. The aim of this approach is to point out the unique and universal, as well as the local and translocal elements in Korean narratives about Potalaka. In other words, the paper searches for patterns that are to be found in the wider Buddhist world and characteristics that are created by the specific religious environment of Korean culture.