bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrating Musculoskeletal Education and Patient Care at Medical Student−Run Free Clinics
Authors: McQuillan Thomas , Wilcox‐Fogel Nate , Kraus Emily , Ladd Amy , Fredericson Michael
Published: April 2017

Background

Student‐run free clinics (SRFCs) have emerged as an important educational component of United States (U.S.) medical schools. Despite the prevalence of musculoskeletal (MSK) problems presenting to SRFCs, students and clinics are often unprepared to diagnose and to treat common MSK complaints.

Objective

We sought to determine the scope of diagnosis and treatment at a medical student−run free clinic specializing in musculoskeletal care using physical medicine and rehabilitation (PM&R) residents. Secondary goals included reviewing student satisfaction and determining the appropriateness of the clinic in medical education.

Design

Retrospective chart review, anonymous online survey.

Setting

Primary care, free student clinic affiliated with tertiary academic medical center.

Participants

A total of 20 medical student volunteers, 6 PM&R residents, and 91 community patients.

Methods

We established a musculoskeletal clinic as a specialty referral clinic for the 2 primary care SRFCs with institutional support from a partner medical school. We then reviewed clinical operations retrospectively using electronic medical records and student satisfaction based on an online survey.

Main Outcome Measurements

We analyzed patient demographics and chief complaints, referrals provided, and medical services rendered. We also used a 5‐point Likert scale to assess student satisfaction.

Results

A monthly musculoskeletal referral clinic was established with the oversight of PM&R attendings and residents. The clinic received 91 referrals and managed 61 unique patients over a 2.5‐year study period. The most common presentations to the clinic involved knee pain (n = 17, 27.9%) and back pain (n = 16, 26.2%). Pro bono relationships with community and institutional partners enabled all patients to receive medical examinations, physical therapy visits, plain film radiographs, and insurance consultations free of charge. Student satisfaction with teaching and patient care was high, with 19 of 20 students reporting their experience as “good” or “excellent.”

Conclusions

SRFCs represent an underused opportunity to enhance MSK education among medical students by treating a variety of common MSK complaints in an underserved population.

Level of Evidence

Not applicable.
The Role of Magnetometry in Managing Arctic Archaeological Sites in the Face of Climate Change
Authors: Hodgetts Lisa M. , Eastaugh Edward J. H.
Published: April 2017

Climate change is impacting archaeological sites around the globe, and Arctic sites are among the most vulnerable because the region is experiencing particularly rapid change. In the face of this threat, archaeologists, heritage managers, and northern communities need to develop strategies for documenting and monitoring Arctic sites and prioritizing them for further investigation. Using three case studies from Banks Island in the western Canadian Arctic, we demonstrate how magnetometer survey could assist in this process, despite the region's poorly developed soils, widespread glacial tills, and periglacial geomorphology, which pose challenges for the technique. The case studies illustrate the utility of magnetometry in mapping both archaeological and permafrost features in the Arctic, allowing it to rapidly investigate site structure and assess the level of threat due to climate change.
A short history of visual literacy: the first five decades
Authors: Michelson Alan
Published: April 2017

The origins and history of Visual literacy (VL) are summarized in this article, from the 1960s writings of John L. Debes, Marshall McLuhan and others of the Rochester School, to the influence of the Internet in the 2000s. ERIC and Google Scholar searches are used to analyse the evolution of its literature over time.
Assessing the impact of library instruction on studio-based research: developing a qualitative model
Authors: Petraits Ellen
Published: April 2017

Throughout the year, research and instruction librarians at Rhode Island School of Design (RISD) instruct students in ways to use the library for studio-based research. At the end of each semester, librarians attend studio critiques for these classes to see the finished work and participate in the critique. These visits are opportunities to look for and reflect upon the presence of research and the impact of concepts taught during library research workshops on the finished presentations and artworks. The coordinator of graduate library instruction created a qualitative tool to assess the evidence of student learning within the studio critique. Its use is cultivating a culture of art and design research within the library and throughout campus by fostering reflection and discussion about the value of qualitative assessment.
‘Untying the Knot’: Achieving Integrative and Collaborative Care within Trauma and Fear Saturated Systems – A Practice-Focused Discussion Paper
Authors: Balu Dilip
Published: April 2017

The author's clinical experience with the Child Protection and Mental Health Care systems informs this brief practice-focused paper. The author posits that Secondary Traumatic Stress and Vicarious Trauma are central to understanding the impact of relationally traumatic material and the experience of individuals, families, team and the wider ecology of care systems. In particular, the author hypothesises that the tendency of systems to become fragmented in operation, with silos of sub-parts working parallel to each other, may be a natural adaptation to the ways in which traumatic experience ripples across system boundaries. This ‘ripple effect’ may lead to increasing emotional and relational reactivity, and survival-oriented inward focus of energies and efforts. The metaphor of the brain and nervous system is used to explore ideas of connection and integration in care systems. Trauma-informed leadership by individuals and teams is also touched upon in relation to reducing fear-driven clinical practice.
To Cut a Long Story Short: Formal Chronological Modelling for the Late Neolithic Site of Ness of Brodgar, Orkney
Authors: Card Nick , Mainland Ingrid , Timpany Scott , Towers Roy , Batt Cathy , Ramsey Christopher Bronk , Dunbar Elaine , Reimer Paula , Bayliss Alex , Marshall Peter , Whittle Alasdair
Published: April 2017

In the context of unanswered questions about the nature and development of the Late Neolithic in Orkney, we present a summary of research up to 2015 on the major site at the Ness of Brodgar, Mainland Orkney, concentrating on the impressive buildings. Finding sufficient samples for radiocarbon dating was a considerable challenge. There are indications, from both features and finds, of activity pre-dating the main set of buildings exposed so far by excavation. Forty-six dates on thirty-nine samples are presented and are interpreted in a formal chronological framework. Two models are presented, reflecting different possible readings of the sequence. Both indicate that piered architecture was in use by the thirtieth century calbcand that the massive Structure 10, not the first building in the sequence, was also in existence by the thirtieth century calbc. Activity associated with piered architecture came to an end (in Model 2) around 2800 calbc. Midden and rubble infill followed. After an appreciable interval, the hearth at the centre of Structure 10 was last used around 2500 calbc, perhaps the only activity in an otherwise abandoned site. The remains of some 400 or more cattle were deposited over the ruins of Structure 10: in Model 2, in the mid-twenty-fifth century calbc, but in Model 1 in the late twenty-fourth or twenty-third century calbc. The chronologies invite comparison with the near-neighbour of Barnhouse, in use from the later thirty-second to the earlier twenty-ninth century calbc, and the Stones of Stenness, probably erected by the thirtieth century calbc. The Ness, including Structure 10, appears to have outlasted Barnhouse, but probably did not endure as long in its primary form as previously envisaged. The decay and decommissioning of the Ness may have coincided with the further development of the sacred landscape around it; but precise chronologies for other sites in the surrounding landscape are urgently required. The spectacular feasting remains of several hundred cattle deposited above Structure 10 may belong to a radically changing world, coinciding (in Model 2) with the appearance of Beakers nationally, but it was arguably the, by now, mythic status of that building which drew people back to it.
Kentucky Bluegrass Invasion Alters Soil Carbon and Vegetation Structure on Northern Mixed-Grass Prairie of the United States
Authors: Sanderson Matt A. , Johnson Holly , Liebig Mark A. , Hendrickson John R. , Duke Sara E.
Published: April 2017

Invasive nonnative grasses pose a significant threat to rangelands of the Northern Great Plains. Long-term data from a grazing experiment near Mandan, ND (46°46′11.43″N, 100°54′55.16″W) revealed the invasion of native prairie by Kentucky bluegrass, an exotic grass. We hypothesized that bluegrass invasion altered soil13C and15N levels, tracking the increased abundance of invasive cool-season grass aboveground. In 2014, soil samples were collected to depths of 0 to 7.6 cm and 7.6 to 15.2 cm in pastures grazed similarly since 1916. Samples were analyzed for total carbon (C) and nitrogen (N) and13C and15N isotopes and compared against archived samples from 1991. Vegetation change from native to exotic grasses changed the isotopic composition of soil C. The soil δ13C at the 0- to 7.6-cm depth became more negative between 1991 and 2014. Soil δ13C became less negative with increasing stocking rate at both soil depths. Soil δ15N values at the 0- to 7.6-cm depth decreased between 1991 and 2014. Soil δ15N increased with increasing stocking rate at the 0- to 7.6-cm depth in 2014. Soil C and N concentrations at 0 to 7.6 cm increased by 35% (12 g C kg−1) and 27% (0.9 g N kg−1), respectively, from 1991 to 2014; however, concentrations at the 7.6- to 15.2-cm depth did not change. The shift from native C4to invasive C3grass did not reduce soil C storage in the long-term prairie pastures. The more deleterious effect of invasion, however, may have been the buildup of dead biomass, which alters vegetation structure and may reduce native species’ diversity and abundance.
Dimensional thinking in psychiatry in the era of the Research Domain Criteria (RDoC)
Authors: Kelly J. R. , Clarke G. , Cryan J. F. , Dinan T. G.
Published: April 2017

The biological mechanisms underlying psychiatric diagnoses are not well defined. Clinical diagnosis based on categorical systems exhibit high levels of heterogeneity and co-morbidity. The Research Domain Criteria (RDoC) attempts to reconceptualize psychiatric disorders into transdiagnostic functional dimensional constructs based on neurobiological measures and observable behaviour. By understanding the underlying neurobiology and pathophysiology of the relevant processes, the RDoC aims to advance biomarker development for disease prediction and treatment response. This important evolving dimensional framework must also consider environmental factors. Emerging evidence suggests that gut microbes (microbiome) play a physiological role in brain diseases by modulating neuroimmune, neuroendocrine and neural signalling pathways between the gut and the brain. The integration of the gut microbiome signature as an additional dimensional component of the RDoC may enhance precision psychiatry.
Turbulent channel flow of a dense binary mixture of rigid particles
Authors: Lashgari Iman , Picano Francesco , Costa Pedro , Breugem Wim-Paul , Brandt Luca
Published: April 2017

We study turbulent channel flow of a binary mixture of finite-sized neutrally buoyant rigid particles by means of interface-resolved direct numerical simulations. We fix the bulk Reynolds number and total solid volume fraction, $Re_{b}=5600$ and $\unicode[STIX]{x1D6F7}=20\,\%$, and vary the relative fraction of small and large particles. The binary mixture consists of particles of two different sizes, $2h/d_{l}=20$ and $2h/d_{s}=30$ where $h$ is the half-channel height and $d_{l}$ and $d_{s}$ the diameters of the large and small particles. While the particulate flow statistics exhibit a significant alteration of the mean velocity profile and turbulent fluctuations with respect to the unladen flow, the differences between the mono-disperse and bi-disperse cases are small. However, we observe a clear segregation of small particles at the wall in binary mixtures, which affects the dynamics of the near-wall region and thus the overall drag. This results in a higher drag in suspensions with a larger number of large particles. As regards bi-disperse effects on the particle dynamics, a non-monotonic variation of the particle dispersion in the spanwise (homogeneous) direction is observed when increasing the percentage of small/large particles. Finally, we note that particles of the same size tend to cluster more at contact whereas the dynamics of the large particles gives the highest collision kernels due to a higher approaching speed.
The Disguises of the Mind: Recent Palestinian Memoirs
Authors: DeYoung Terri
Published: April 2017

This article examines developments in the memoir form in Palestinian literature over time, concentrating on the period since the Oslo Accords of 1993. It begins by briefly looking at new, general theoretical perspectives on the memoir form relevant to recognizing the importance of memoirs by Palestinians. A shift from viewing memoirs as purely the product of individuals to validating the notion ofcollectiverecollection occurs in the theoretical literature and can be documented in the Palestinian memoir. Similarly, attention to the idea of recollection asfluidand as aprocessenriches our understanding of what Palestinian memorists are trying to achieve. This section draws upon the work of such foundational figures as Maurice Halbwachs, Paul Ricoeur, Sidonie Smith, and Julia Watson. It also incorporates the insights of those who have focused more specifically on the Palestinian memoir form, such as Rashid Khalidi and Susan Slyomovics. These theoretical views are then tested through the examination of fourteen works using the memoir format, ranging from the time of the dissolution of the Palestinian Mandate (Sakkakini, Jabra, Boullata) to the beginning of the new millennium and the decade beyond (Darwish, Barghouti, Amiry and Karmi). The article shows that any literary history of the memoir form in modern times would be incomplete without recognizing and incorporating the Palestinian contribution to this increasingly popular literary genre.
Influence of Hegenshan–Heihe suture on evolution of late Mesozoic extensional structures in Wunite depression, Erlian Basin, Inner Mongolia, China
Authors: MIAO QUANYUN , QI JIAFU , WANG LISHUANG , ZHANG SHUAI , WEI XIULI
Published: April 2017

We integrate previous work on the Hegenshan–Heihe suture with our interpretation of geomagnetic anomaly and seismic reflection data to investigate the role of the Hegenshan–Heihe suture in the evolution of late Mesozoic extensional structures in the Wunite depression of the Erlian Basin. Sags in the Wunite depression present as NE50° trending in the western sector, N0°–NE30° trending in the central sector and NE45° trending in the eastern sector. Our results highlight the importance of the pre-existing Hegenshan–Heihe suture in the evolution of the late Mesozoic rift system and reveal the following details. (1) The NE50° extent sags in the western sector are controlled by the c. NE50°-trending suture. Moreover, the extensional deformation of the reactivated suture during Early Cretaceous time resulted in a further vertical and horizontal extension of major border faults. (2) The N0°–NE30° extent sags in the central sector are influenced by the c. NE75°-trending suture. The sinistral strike-slip component of the reactivated suture during Early Cretaceous time resulted in a strike rotation of major border faults from NEE-trending (following the suture) to NNE-trending. (3) Because of strike-slip deformation, which resulted from the deformation of the reactivated suture accrued in major border faults, light dip-slip deformation led to less vertical offset. (4) The NE45°-trending sags in the eastern sector are controlled by the c. NE45°-trending suture. Moreover, the extensional deformation of the reactivated suture during Early Cretaceous time facilitated a further vertical and horizontal prolongation of major border faults.
Discourse analysis of the 2013–2016 Arctic Circle Assembly programmes
Authors: Johannsdottir Lara , Cook David
Published: April 2017

The Arctic Circle Assembly has established itself as a forum for stakeholders from all around the globe to discuss Arctic affairs. This includes discussion about the future of the Arctic, impacts of climate change on local inhabitants and indigenous peoples, the natural environment and wildlife, geopolitical issues, international treaties, research projects, business-related risks and opportunities, etc. At this international conference, the interests of stakeholders vary greatly. Some want to reap the economic and strategic benefits of melting glacial and sea ice, while others want to reduce the negative impacts of climate change. It is therefore important to analyse the conference discourse in order to understand the main emphases of stakeholders, and if some express their views more loudly than others. Through greater weight of voice in the Assembly and beyond, some stakeholders have more impact on the development of a region that is of economic, geopolitical and environmental importance, not only for the northern hemisphere but globally.
Who Wants Demanding Active Labour Market Policies? Public Attitudes towards Policies that put Pressure on the Unemployed
Authors: FOSSATI FLAVIA
Published: April 2017

The literature addressing attitudes about social policy and the welfare state has been telling us for decades that welfare interventions are supported by those individuals who benefit from a specific measure. The diffusion of ‘demanding’ active labour market policies (ALMPs), however, challenges this relationship. Using a novel dataset, I analyse which individual- and country-level factors explain public support for demanding ALMPs in five Western European countries. The results show that labour market risk and ideological orientation influence public attitudes towards these ALMPs. Thereby, unemployed individuals sympathising with the political right are more strongly opposed to demanding measures than employed individuals with the same political preferences. Moreover, aggregate support is found to be correlated with the country's ALMP legacy, varying from high levels in Germany and the UK to low levels in Denmark and France. The findings suggest that most ALMPs are in fact implemented despite the opposition of their beneficiaries.
Investigation of vitamin D status and its correlation with insulin resistance in a Chinese population
Authors: Han Bing , Wang Xiaojin , Wang Ningjian , Li Qin , Chen Yi , Zhu Chunfang , Chen Yingchao , Xia Fangzhen , Pu Xiaoqi , Cang Zhen , Zhu Chaoxia , Lu Meng , Meng Ying , Guo Hui , Chen Chi , Tu Weiping , Li Bin , Hu Ling , Wang Bingshun , Lu Yingli
Published: April 2017

Objective

Although many studies worldwide have focused on the relationship between vitamin D and insulin resistance, results remain controversial. Furthermore, concentrations of serum 25-hydroxyvitamin D (25(OH)D) in the Chinese population are unclear. We aimed to investigate vitamin D status and its correlation with insulin resistance among a Chinese adult population.

Design

Serum 25(OH)D, fasting blood glucose, fasting insulin, glycated Hb (HbA1c) and other metabolic parameters were assessed. Neck circumference, waist circumference, hip circumference, weight and height were also measured. Lifestyle factors including smoking and drinking status were obtained. Diabetes mellitus was diagnosed by HbA1c according to the 2010 American Diabetes Association criteria.

Setting

Eastern China.

Subjects

Of 7200 residents included, 6597 individuals were ultimately analysed.

Results

We enrolled 2813 males (mean age 52·7 (sd 13·5) years) and 3784 females (52·3 (sd 13·5) years); mean serum 25(OH)D concentration was 43·1 (sd 11·6) and 39·6 (sd 9·8) nmol/l, respectively. Additionally, 83·3 % of participants were 25(OH)D deficient. A significant difference in 25(OH)D was observed between males and females in winter and spring (P<0·001). Furthermore, 25(OH)D concentrations were inversely associated with the homeostasis model assessment of insulin resistance (HOMA-IR) in the overweight and pre-diabetic populations. After adjusting for several variables, 25(OH)D was significantly associated with HOMA-IR in winter. When 25(OH)D values were categorized into quartiles, HOMA-IR was significantly associated with decreasing 25(OH)D.

Conclusions

The majority of the Chinese population was vitamin D deficient and this deficiency was negatively associated with insulin resistance, particularly in the overweight and pre-diabetic populations. Moreover, these associations might be more evident in the winter.
Stability of a Bifunctional Cu-Based Core@Zeolite Shell Catalyst for Dimethyl Ether Synthesis Under Redox Conditions Studied by Environmental Transmission Electron Microscopy andIn SituX-Ray Ptychography
Authors: Baier Sina , Damsgaard Christian D. , Klumpp Michael , Reinhardt Juliane , Sheppard Thomas , Balogh Zoltan , Kasama Takeshi , Benzi Federico , Wagner Jakob B. , Schwieger Wilhelm , Schroer Christian G. , Grunwaldt Jan-Dierk
Published: April 2017

When using bifunctional core@shell catalysts, the stability of both the shell and core–shell interface is crucial for catalytic applications. In the present study, we elucidate the stability of a CuO/ZnO/Al2O3@ZSM-5 core@shell material, used for one-stage synthesis of dimethyl ether from synthesis gas. The catalyst stability was studied in a hierarchical manner by complementary environmental transmission electron microscopy (ETEM), scanning electron microscopy (SEM) andin situhard X-ray ptychography with a specially designedin situcell. Both reductive activation and reoxidation were applied. The core–shell interface was found to be stable during reducing and oxidizing treatment at 250°C as observed by ETEM andin situX-ray ptychography, although strong changes occurred in the core on a 10 nm scale due to the reduction of copper oxide to metallic copper particles. At 350°C,in situX-ray ptychography indicated the occurrence of structural changes also on theµm scale, i.e. the core material and parts of the shell undergo restructuring. Nevertheless, the crucial core–shell interface required for full bifunctionality appeared to remain stable. This study demonstrates the potential of these correlativein situmicroscopy techniques for hierarchically designed catalysts.
Liquid authority and political legitimacy in transnational governance
Authors: Macdonald Kate , Macdonald Terry
Published: April 2017

In this article we investigate the institutional mechanisms required for ‘liquid’ forms of authority in transnational governance to achieve normative political legitimacy. We understand authority in sociological terms as the institutionalized inducement of addressees to defer to institutional rules, directives, or knowledge claims. We take authority to be ‘liquid’ when it is characterized by significant institutional dynamism, fostered by its informality, multiplicity, and related structural properties. The article’s central normative claim is that the mechanisms prescribed to legitimize transnational governance institutions – such as accountability or experimentalist mechanisms – should vary with the liquid characteristics of their authority structures. We argue for this claim in two steps. We first outline our theoretical conception of political legitimacy – as a normative standard prescribing legitimizing mechanisms that support authorities’ collectively valuable governance functions – and we explain in theoretical terms why legitimizing mechanisms should vary with differing authority structures. We then present an illustrative case study of the interaction between liquid authority and legitimizing mechanisms of public accountability and pragmatic experimentalism in the context of transnational business regulation. We conclude by considering broader implications of our argument for both the design of legitimate transnational governance institutions, and future research agendas on transnational authority and legitimacy.
Follistatin‐like protein 1 modulates IL‐17 signaling via IL‐17RC regulation in stromal cells
Authors: Campfield Brian T , Eddens Taylor , Henkel Matthew , Majewski Martin , Horne William , Chaly Yury , Gaffen Sarah L , Hirsch Raphael , Kolls Jay K
Published: April 2017

Follistatin‐like protein 1 (FSTL‐1) possesses several newly identified roles in mammalian biology, including interleukin (IL)‐17‐driven inflammation, though the mechanism underlying FSTL‐1 influence on IL‐17‐mediated cytokine production is unknown. Using parallel in vitro bone marrow stromal cell models of FSTL‐1 suppression, we employed unbiased microarray analysis to identify FSTL‐1‐regulated genes and pathways that could influence IL‐17‐dependent production of IL‐6 and granulocyte colony‐stimulating factor. We discovered that FSTL‐1 modulates Il17rc gene expression. Specifically, FSTL‐1 was necessary for Il17rc gene transcription, IL‐17RC surface protein expression and IL‐17‐dependent cytokine production. This work identifies a mechanism by which FSTL‐1 influences IL‐17‐driven inflammatory signaling in vitro and reveals a novel function for FSTL‐1, as a modulator of gene expression. Thus enhanced understanding of the interplay between FSTL‐1 and IL‐17‐mediated inflammation may provide insight into potential therapeutic targets of IL‐17‐mediated diseases and warrants ongoing study of in vivo models and clinical scenarios of FSTL‐1‐influenced diseases.
Controlling the orbital angular momentum of high harmonic vortices
Authors: Kong Fanqi , Zhang Chunmei , Bouchard Frédéric , Li Zhengyan , Brown Graham G. , Ko Dong Hyuk , Hammond T. J. , Arissian Ladan , Boyd Robert W. , Karimi Ebrahim , Corkum P. B.
Published: April 2017

Optical vortices, which carry orbital angular momentum (OAM), can be flexibly produced and measured with infrared and visible light. Their application is an important research topic for super-resolution imaging, optical communications and quantum optics. However, only a few methods can produce OAM beams in the extreme ultraviolet (XUV) or X-ray, and controlling the OAM on these beams remains challenging. Here we apply wave mixing to a tabletop high-harmonic source, as proposed in our previous work, and control the topological charge (OAM value) of XUV beams. Our technique enables us to produce first-order OAM beams with the smallest possible central intensity null at XUV wavelengths. This work opens a route for carrier-injected laser machining and lithography, which may reach nanometre or even angstrom resolution. Such a light source is also ideal for space communications, both in the classical and quantum regimes.
Kinetic CRAC uncovers a role for Nab3 in determining gene expression profiles during stress
Authors: van Nues Rob , Schweikert Gabriele , de Leau Erica , Selega Alina , Langford Andrew , Franklin Ryan , Iosub Ira , Wadsworth Peter , Sanguinetti Guido , Granneman Sander
Published: April 2017

RNA-binding proteins play a key role in shaping gene expression profiles during stress, however, little is known about the dynamic nature of these interactions and how this influences the kinetics of gene expression. To address this, we developed kinetic cross-linking and analysis of cDNAs (χCRAC), an ultraviolet cross-linking method that enabled us to quantitatively measure the dynamics of protein–RNA interactions in vivo on a minute time-scale. Here, using χCRAC we measure the global RNA-binding dynamics of the yeast transcription termination factor Nab3 in response to glucose starvation. These measurements reveal rapid changes in protein–RNA interactions within 1 min following stress imposition. Changes in Nab3 binding are largely independent of alterations in transcription rate during the early stages of stress response, indicating orthogonal transcriptional control mechanisms. We also uncover a function for Nab3 in dampening expression of stress-responsive genes. χCRAC has the potential to greatly enhance our understanding of in vivo dynamics of protein–RNA interactions.
Precise treatment of aortic aneurysm by three-dimensional printing and simulation before endovascular intervention
Authors: Yuan Ding , Luo Han , Yang Hongliu , Huang Bin , Zhu Jingqiang , Zhao Jichun
Published: April 2017

In this study, three-dimensional printing (3Dp) models and simulation surgeries (SSs) were applied in two challenging aortic cases. The first was an abdominal aortic aneurysm with a complex neck, and the second was a thoracic aortic dissection aneurysm (TADA) with an angled arch. In order to avoid unpredictable obstacles and difficulties, we made optimal surgical plans by using 3D models and virtual simulations. Based on preoperative evaluation system, the surgical plans seemed more reasonable and time-saving.
Maternal plasma miRNAs as biomarkers during mid-pregnancy to predict later spontaneous preterm birth: a pilot study
Authors: Gray Clint , McCowan Lesley M. , Patel Rachna , Taylor Rennae S. , Vickers Mark H.
Published: April 2017

More than 10% of babies are born too early resulting in over 15 million preterm births and more than one million new-born deaths globally. Although women with a previous spontaneous preterm birth (SPTB) are considered at high risk for recurrence, the majority occur in women without prior history. Prediction of SPTB risk allows for improved care and potential for targeting novel and existing therapeutics to prevent SPTB, which may result in improved outcomes for infant and mother. In this pilot study, a miRNA array was used to analyse plasma from healthy women in their first pregnancy at 20 weeks of gestation who then went on to deliver either at term or experience SPTB at 28–32 weeks. We identified specific miRNA expression profiles that differentiated between those mothers who delivered at term or delivered following SPTB. miR302b, miR1253 and a clustering of miR548 miRNAs were underexpressed in SPTB cases compared to term controls. Conversely, miR223 was elevated in mothers that later experienced a SPTB. The circulating miRNAs identified in the present study may therefore be attractive candidates as non-invasive biomarkers for the early prediction of SPTB. Further larger studies are now warranted to investigate the potential clinical utility of these markers.
Prediction of Bacterial Contamination Outbursts in Water Wells through Sparse Coding
Authors: Frolich Levi , Vaizel-Ohayon Dalit , Fishbain Barak
Published: April 2017

Maintaining water quality is critical for any water distribution company. One of the major concerns in water quality assurance, is bacterial contamination in water sources. To date, bacteria growth models cannot predict with sufficient accuracy when a bacteria outburst will occur in a water well. This is partly due to the natural sparsity of the bacteria count time series, which hinders the observation of deviations from normal behavior. This precludes the application of mathematical models nor statistical quality control methods for the detection of high bacteria counts before contamination occurs. As a result, currently a future outbreak prediction is a subjective process. This research developed a new cost-effective method that capitalizes on the sparsity of the bacteria count time series. The presented method first transforms the data into its spectral representation, where it is no longer sparse. Capitalizing on the spectral representation the dimensions of the problem are reduced. Machine learning methods are then applied on the reduced representations for predicting bacteria outbursts from the bacterial counts history of a well. The results show that these tools can be implemented by the water quality engineering community to create objective, more robust, quality control techniques to ensure safer water distribution.
Effect of surface state hybridization on current-induced spin-orbit torque in thin topological insulator films
Authors: Ho Cong Son , Wang Yi , Siu Zhou Bin , Tan Seng Ghee , Jalil Mansoor B. A. , Yang Hyunsoo
Published: April 2017

We investigate the thickness optimization for maximum current-induced spin-orbit torque (SOT) generated by topological surface states (TSS’s) in a bilayer system comprising of a ferromagnetic layer coupled to a thin topological insulator (TI) film. We show that by reducing the TI thickness, two competing effects on the SOT are induced: (i) the torque strength is stronger as the bulk contribution is decreased; (ii) on the other hand, the torque strength becomes suppressed due to increasing hybridization of the surface states. The latter is attributed to the opposite helicities of the coupled TSS’s. We theoretically model the interplay of these two effects and derive the optimal TI thickness to maximize the spin torque, which is estimated to be about 3–5 nm for typical Bi2Se3 films.
Rational development of Stafib-2: a selective, nanomolar inhibitor of the transcription factor STAT5b
Authors: Elumalai Nagarajan , Berg Angela , Rubner Stefan , Blechschmidt Linda , Song Chen , Natarajan Kalaiselvi , Matysik Jörg , Berg Thorsten
Published: April 2017

The transcription factor STAT5b is a target for tumour therapy. We recently reported catechol bisphosphate and derivatives such as Stafib-1 as the first selective inhibitors of the STAT5b SH2 domain. Here, we demonstrate STAT5b binding of catechol bisphosphate by solid-state nuclear magnetic resonance, and report on rational optimization of Stafib-1 (Ki = 44 nM) to Stafib-2 (Ki = 9 nM). The binding site of Stafib-2 was validated using combined isothermal titration calorimetry (ITC) and protein point mutant analysis, representing the first time that functional comparison of wild-type versus mutant protein by ITC has been used to characterize the binding site of a small-molecule ligand of a STAT protein with amino acid resolution. The prodrug Pomstafib-2 selectively inhibits tyrosine phosphorylation of STAT5b in human leukaemia cells and induces apoptosis in a STAT5-dependent manner. We propose Pomstafib-2, which currently represents the most active, selective inhibitor of STAT5b activation available, as a chemical tool for addressing the fundamental question of which roles the different STAT5 proteins play in various cell processes.
Nano silica diaphragm in-fiber cavity for gas pressure measurement
Authors: Liu Shen , Wang Yiping , Liao Changrui , Wang Ying , He Jun , Fu Cailing , Yang Kaiming , Bai Zhiyong , Zhang Feng
Published: April 2017

We demonstrate an ultrahigh-sensitivity gas pressure sensor based on the Fabry-Perot interferometer employing a fiber-tip diaphragm-sealed cavity. The cavity is comprised of a silica capillary and ultrathin silica diaphragm with a thickness of 170 nm, with represents the thinnest silica diaphragm fabricated thus far by an electrical arc discharge technique. The resulting Fabry-Perot interferometer-based gas pressure sensor demonstrates a gas pressure sensitivity of about 12.22 nm/kPa, which is more than two orders of magnitude greater than that of a similarly configured fiber-tip air bubble sensor. Moreover, our gas pressure sensor has a low temperature cross-sensitivity of about 106 Pa/°C, and the sensor functions well up to a temperature of about 1000 °C. As such, the sensor can potentially be employed in high-temperature environments.
Maternal lipids and leptin concentrations are associated with large-for-gestational-age births: a prospective cohort study
Authors: Farias Dayana Rodrigues , Poston Lucilla , Franco-Sena Ana Beatriz , Moura da Silva Antônio Augusto , Pinto Thatiana , de Oliveira Lívia Costa , Kac Gilberto
Published: April 2017

The change in maternal lipid, leptin and adiponectin concentrations during pregnancy and infant birth weight (BW) is still poorly characterized. Thus, the aim of the study was to evaluate the association of maternal lipids, leptin and adiponectin throughout pregnancy with large-for-gestational-age (LGA) births and BW z-score. A prospective cohort of 199 mothers was followed during pregnancy in Rio de Janeiro, Brazil. The statistical analyses comprised multiple logistic and linear regression. Women delivered 36 LGA and 11 small-for-gestational-age newborns. HDL-c rate of change throughout pregnancy was negatively associated with BW z-score (β = −1.99; p = 0.003) and the delivery of a LGA newborn (OR = 0.02; p = 0.043). Pregnancy baseline concentration of log leptin was positively associated (OR = 3.92; p = 0.025) with LGA births. LDL-c rate of change throughout pregnancy was positively associated with BW z-score (β = 0.31; p = 0.004). Log triglycerides and log adiponectin were not significantly associated with BW z-score or LGA birth. In conclusion, a higher log leptin pregnancy baseline concentration and a lower HDL-c rate of change during pregnancy were associated with higher odds of having a LGA newborn. These maternal biomarkers are important to foetal growth and could be used in prenatal care as an additional strategy to screen women at risk of inadequate BW.
Investigation of spin-orbit torque using current-induced magnetization curve
Authors: Koyama Tomohiro , Guan Yicheng , Chiba Daichi
Published: April 2017

Manipulation of magnetization using current-induced torque is crucial for magnetic recording devices. Recently, the spin-orbit torque (SOT) that emerges in a ferromagnetic thin film on a heavy metal is focused as a new scheme for magnetization switching in perpendicularly magnetized systems. Since the SOT provides a perpendicular effective field to the system, the formation of a magnetic multiple domain state because of Joule heating is supressed in the magnetization reversal process. This means that high reliable switching is possible using the SOT. Here, by utilizing the SOT induced domain stability, we show that an electrical current directly injected to a perpendicularly magnetized Pt/Co/Pd system can magnetize itself, that is, current-induced magnetization process from multi to single domain state. A quantitative determination of the SOT is performed using the current-induced magnetization curve. The present results are of great importance as another approach to evaluate the SOT effect, as well as a demonstration of domain state switching caused by the SOT.
Tropicalization strengthens consumer pressure on habitat-forming seaweeds
Authors: Zarco-Perello Salvador , Wernberg Thomas , Langlois Tim J. , Vanderklift Mathew A.
Published: April 2017

Ocean warming is driving species poleward, causing a ‘tropicalization’ of temperate ecosystems around the world. Increasing abundances of tropical herbivores on temperate reefs could accelerate declines in habitat-forming seaweeds with devastating consequences for these important marine ecosystems. Here we document an expansion of rabbitfish (Siganus fuscescens), a tropical herbivore, on temperate reefs in Western Australia following a marine heatwave and demonstrate their impact on local kelp forests (Ecklonia radiata). Before the heatwave there were no rabbitfish and low rates of kelp herbivory but after the heatwave rabbitfish were common at most reefs and consumption of kelp was high. Herbivory increased 30-fold and kelp abundance decreased by 70% at reefs where rabbitfish had established. In contrast, where rabbitfish were absent, kelp abundance and herbivory did not change. Video-analysis confirmed that rabbitfish were the main consumers of kelp, followed by silver drummers (Kyphosus sydneyanus), a temperate herbivore. These results represent a likely indirect effect of the heatwave beyond its acute impacts, and they provide evidence that range-shifting tropical herbivores can contribute to declines in habitat-forming seaweeds within a few years of their establishment.
Global evolutionary history and spatio-temporal dynamics of dengue virus type 2
Authors: Wei Kaifa , Li Yuhan
Published: April 2017

DENV-2 spread throughout the tropical and subtropical regions globally, which is implicated in deadly outbreaks of DHF and DSS. Since dengue cases have grown dramatically in recent years, about half of the world’s population is now at risk. Our timescale analysis indicated that the most recent common ancestor existed about 100 years ago. The rate of nucleotide substitution was estimated to be 8.94 × 10−4subs/site/year. Selection pressure analysis showed that two sites 160 and 403 were under positive selection, while E gene is mainly shaped by stronger purifying selection. BSP analysis showed that estimating effective population size from samples of sequences has undergone three obvious increases, additionally, Caribbean and Puerto Rico maintained higher levels of genetic diversity relative to other 6 representative geographical populations using GMRF method. The phylogeographic analysis indicated that two major transmission routes are from South America to Caribbean and East&SouthAsia to Puerto Rico. The trunk reconstruction confirmed that the viral evolution spanned 50 years occurred primarily in Southeast Asia and East&South Asia. In addition, phylogeographic association-trait analysis indicated that the viral phenotypes are highly correlated with phylogeny in Nicaragua and Puerto Rico (P < 0.05).
Novel micelle PCR-based method for accurate, sensitive and quantitative microbiota profiling
Authors: Boers Stefan A. , Hays John P. , Jansen Ruud
Published: April 2017

In the last decade, many researchers have embraced 16S rRNA gene sequencing techniques, which has led to a wealth of publications and documented differences in the composition of microbial communities derived from many different ecosystems. However, comparison between different microbiota studies is currently very difficult due to the lack of a standardized 16S rRNA gene sequencing protocol. Here we report on a novel approach employing micelle PCR (micPCR) in combination with an internal calibrator that allows for standardization of microbiota profiles via their absolute abundances. The addition of an internal calibrator allows the researcher to express the resulting operational taxonomic units (OTUs) as a measure of 16S rRNA gene copies by correcting the number of sequences of each individual OTU in a sample for efficiency differences in the NGS process. Additionally, accurate quantification of OTUs obtained from negative extraction control samples allows for the subtraction of contaminating bacterial DNA derived from the laboratory environment or chemicals/reagents used. Using equimolar synthetic microbial community samples and low biomass clinical samples, we demonstrate that the calibrated micPCR/NGS methodology possess a much higher precision and a lower limit of detection compared with traditional PCR/NGS, resulting in more accurate microbiota profiles suitable for multi-study comparison.
Silk-fibronectin protein alloy fibres support cell adhesion and viability as a high strength, matrix fibre analogue
Authors: Jacobsen Matthew M. , Li David , Gyune Rim Nae , Backman Daniel , Smith Michael L. , Wong Joyce Y.
Published: April 2017

Silk is a natural polymer with broad utility in biomedical applications because it exhibits general biocompatibility and high tensile material properties. While mechanical integrity is important for most biomaterial applications, proper function and integration also requires biomaterial incorporation into complex surrounding tissues for many physiologically relevant processes such as wound healing. In this study, we spin silk fibroin into a protein alloy fibre with whole fibronectin using wet spinning approaches in order to synergize their respective strength and cell interaction capabilities. Results demonstrate that silk fibroin alone is a poor adhesive surface for fibroblasts, endothelial cells, and vascular smooth muscle cells in the absence of serum. However, significantly improved cell attachment is observed to silk-fibronectin alloy fibres without serum present while not compromising the fibres’ mechanical integrity. Additionally, cell viability is improved up to six fold on alloy fibres when serum is present while migration and spreading generally increase as well. These findings demonstrate the utility of composite protein alloys as inexpensive and effective means to create durable, biologically active biomaterials.
Off-target effects and clinical outcome in metastatic colorectal cancer patients receiving regorafenib: The TRIBUTE analysis
Authors: Giampieri Riccardo , Prete Michela del , Prochilo Tiziana , Puzzoni Marco , Pusceddu Valeria , Pani Fabiana , Maccaroni Elena , Mascia Roberta , Baleani Maria Giuditta , Meletani Tania , Berardi Rossana , Lanzillo Anna Maria , Mariotti Stefano , Zaniboni Alberto , Cascinu Stefano , Scartozzi Mario
Published: April 2017

Regorafenib is an orally administered multikinase inhibitor indicated for the treatment of heavily pretreated metastatic colorectal cancer patients with good performance status, albeit less than 50% treated patients achieve disease stabilisation or better at the first radiological evaluation. In addition to that a particularly broad spectrum of toxicities (experienced as G3 or more NCI CTCAE graded by 50% of patients treated) have led to reconsider its widespread use in the majority of patients. We retrospectively collected data about the magnitude of off-target effects experienced during the first 8-weeks of regorafenib monotherapy and analysed their correlation with overall survival, progression free survival and disease control rate. Our findings suggest that skin rash (Exp (B): 0.52, p = 0.0133) or hypothyroidism (Exp (B): 0.11, p = 0.0349) were significantly correlated with improved overall survival at multivariate regression analysis. It was also demonstrated a statistically significant role of diarrhea as predictor of improved survival but its independent prognostic role was lost at multivariate analysis (Exp (B): 0.63, p = 0.162). This is the first analysis showing a potential correlation between the onset of these forms of side effects and regorafenib efficacy, however sample size limitations and the retrospective nature of our analysis prevent us from drawing definitive conclusions.
Spin-resolved band structure of heterojunction Bi-bilayer/3D topological insulator in the quantum dimension regime in annealed Bi2Te2.4Se0.6
Authors: Klimovskikh I. I. , Sostina D. , Petukhov A. , Rybkin A. G. , Eremeev S. V. , Chulkov E. V. , Tereshchenko O. E. , Kokh K. A. , Shikin A. M.
Published: April 2017

Two- and three-dimensional topological insulators are the key materials for the future nanoelectronic and spintronic devices and quantum computers. By means of angle- and spin-resolved photoemission spectroscopy we study the electronic and spin structure of the Bi-bilayer/3D topological insulator in quantum tunneling regime formed under the short annealing of Bi2Te2.4Se0.6. Owing to the temperature-induced restructuring of the topological insulator’s surface quintuple layers, the hole-like spin-split Bi-bilayer bands and the parabolic electronic-like state are observed instead of the Dirac cone. Scanning Tunneling Microscopy and X-ray Photoemission Spectroscopy measurements reveal the appearance of the Bi2 terraces at the surface under the annealing. The experimental results are supported by density functional theory calculations, predicting the spin-polarized Bi-bilayer bands interacting with the quintuple-layers-derived states. Such an easily formed heterostructure promises exciting applications in spin transport devices and low-energy electronics.