bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimizing the Comet Assay-Based In Vitro DNA Repair Assay for Placental Tissue: A Pilot Study with Pre-Eclamptic Patients
Authors: Mircheva Anastasiya , Vangrieken Philippe , Al-Nasiry Salwan , van Schooten Frederik-Jan , Godschalk Roger W. L. , Langie Sabine A. S.
Published: December 2023

The comet assay-based in vitro DNA repair assay has become a common tool for quantifying base excision repair (BER) activity in human lymphocytes or cultured cells. Here, we optimized the protocol for studying BER in human placental tissue because the placenta is a non-invasive tissue for biomonitoring of early-life exposures, and it can be used to investigate molecular mechanisms associated with prenatal disorders. The optimal protein concentration of placental protein extracts for optimal damage recognition and incision was 2 mg protein/mL. The addition of aphidicolin did not lead to reduced non-specific incisions and was, therefore, not included in the optimized protocol. The interval between sample collection and analysis did not affect BER activity up to 70 min. Finally, this optimized protocol was tested on pre-eclamptic (PE) placental tissues (n = 11) and significantly lower BER activity in PE placentas compared to controls (n = 9) was observed. This was paralleled by a significant reduction in the expression of BER-related genes and increased DNA oxidation in PE placentas. Our study indicates that BER activity can be determined in placentas, and lower activity is present in PE compared with healthy. These findings should be followed up in prospective clinical investigations to examine BER’s role in the advancement of PE.
Metabolomic Insight into Implications of Induction Chemotherapy Followed by Concomitant Chemoradiotherapy in Locally Advanced Head and Neck Cancer
Authors: Boguszewicz Łukasz , Bieleń Agata , Ciszek Mateusz , Skorupa Agnieszka , Mrochem-Kwarciak Jolanta , Składowski Krzysztof , Sokół Maria
Published: December 2023

The present study compares two groups of locally advanced patients with head and neck squamous cell carcinoma (LA-HNSCC) undergoing concurrent chemoradiotherapy (cCHRT), specifically those for whom it is a first-line treatment and those who have previously received induction chemotherapy (iCHT). The crucial question is whether iCHT is a serious burden during subsequent treatment for LA-HNSCC and how iCHT affects the tolerance to cCHRT. Of the 107 LA-HNSCC patients, 54 received cisplatin-based iCHT prior to cCHRT. The patients were clinically monitored at weekly intervals from the day before until the completion of the cCHRT. The 843 blood samples were collected and divided into two aliquots: for laboratory blood tests and for nuclear magnetic resonance (NMR) spectroscopy (a Bruker 400 MHz spectrometer). The NMR metabolites and the clinical parameters from the laboratory blood tests were analyzed using orthogonal partial least squares analysis (OPLS) and the Mann–Whitney U test (MWU). After iCHT, the patients begin cCHRT with significantly (MWU p-value < 0.05) elevated blood serum lipids, betaine, glycine, phosphocholine, and reticulocyte count, as well as significantly lowered NMR inflammatory markers, serine, hematocrit, neutrophile, monocyte, red blood cells, hemoglobin, and CRP. During cCHRT, a significant increase in albumin and psychological distress was observed, as well as a significant decrease in platelet, N-acetyl-cysteine, tyrosine, and phenylalanine, in patients who received iCHT. Importantly, all clinical symptoms (except the decreased platelets) and most metabolic alterations (except for betaine, serine, tyrosine, glucose, and phosphocholine) resolve until the completion of cCHRT. In conclusion, iCHT results in hematological toxicity, altered lipids, and one-carbon metabolism, as well as downregulated inflammation, as observed at the beginning and during cCHRT. However, these complications are temporary, and most of them resolve at the end of the treatment. This suggests that iCHT prior to cCHRT does not pose a significant burden and should be considered as a safe treatment option for LA-HNSCC.
Principal Component Analysis (PCA) of Molecular Descriptors for Improving Permeation through the Blood–Brain Barrier of Quercetin Analogues
Authors: Pavlović Nebojša , Milošević Sopta Nastasija , Mitrović Darko , Zaklan Dragana , Tomas Petrović Ana , Stilinović Nebojša , Vukmirović Saša
Published: December 2023

Despite its beneficial pharmacological effects in the brain, partly by modulating inositol phosphate multikinase (IPMK) activity, the therapeutic use of quercetin is limited due to its poor solubility, low oral bioavailability, and low permeability through the blood–brain barrier (BBB). We aimed to identify quercetin analogues with improved BBB permeability and preserved binding affinities towards IPMK and to identify the molecular characteristics required for them to permeate the BBB. Binding affinities of quercetin analogues towards IPMK were determined by molecular docking. Principal component analysis (PCA) was applied to identify the molecular descriptors contributing to efficient permeation through the BBB. Among 34 quercetin analogues, 19 compounds were found to form more stable complexes with IPMK, and the vast majority were found to be more lipophilic than quercetin. Using two distinct in silico techniques, insufficient BBB permeation was determined for all quercetin analogues. However, using the PCA method, the descriptors related to intrinsic solubility and lipophilicity (logP) were identified as mainly responsible for clustering four quercetin analogues (trihydroxyflavones) with the highest BBB permeability. The application of PCA revealed that quercetin analogues could be classified with respect to their structural characteristics, which may be utilized in further analogue syntheses and lead optimization of BBB-penetrating IPMK modulators as neuroprotective agents.
Medical Device-Associated Healthcare Infections: Sterilization and the Potential of Novel Biological Approaches to Ensure Patient Safety
Authors: Garvey Mary
Published: December 2023

Healthcare-associated infections caused by multi-drug-resistant pathogens are increasing globally, and current antimicrobial options have limited efficacy against these robust species. The WHO details the critically important bacterial and fungal species that are often associated with medical device HAIs. The effective sterilization of medical devices plays a key role in preventing infectious disease morbidity and mortality. A lack of adherence to protocol and limitations associated with each sterilization modality, however, allows for the incidence of disease. Furthermore, issues relating to carcinogenic emissions from ethylene oxide gas (EtO) have motivated the EPA to propose limiting EtO use or seeking alternative sterilization methods for medical devices. The Food and Drug Administration supports the sterilization of healthcare products using low-temperature VH2O2 as an alternative to EtO. With advances in biomaterial and medical devices and the increasing use of combination products, current sterilization modalities are becoming limited. Novel approaches to disinfection and sterilization of medical devices, biomaterials, and therapeutics are warranted to safeguard public health. Bacteriophages, endolysins, and antimicrobial peptides are considered promising options for the prophylactic and meta-phylactic control of infectious diseases. This timely review discusses the application of these biologics as antimicrobial agents against critically important WHO pathogens, including ESKAPE bacterial species.
Phenotypic Variation in Flower Color and Morphology in the Gerbera (Gerbera hybrida) F1 Hybrid Population and Their Association with EST-SSR Markers
Authors: Zhou Yiwei , Zou Xinru , Yan Fulong , He Jingjuan , Zeng Sixian , Yu Yunyi , Tang Xiaoshuang , Liang Xuanguo , Cai Xiuping , Yu Rangcai , Fan Yanping
Published: December 2023

Gerbera (Gerbera hybrida) is a widely cultivated ornamental plant. However, its genetic improvement is limited by the lack of genetic analysis and molecular markers for traits. In this study, we analyzed the phenotypic and genotypic variation of 140 F1 progeny from two gerbera varieties with different flower types and colors. We evaluated the flower’s morphology, color, and pigment content of the F1 population and performed cluster principal component analysis (PCA) and correlation analysis. The results showed that the main ornamental traits of the hybrid progeny varied greatly. The segregation ratios of single and double flowers and ligulate and split ray florets were both 1:1. The flower colors of the F1 progeny were mainly red and purple-red, similar to the male parent’s color. Furthermore, we conducted a genetic analysis of the hybrid progeny using EST-SSR markers and performed association analysis with phenotypic traits. We identified 2, 2, 3, 1, and 2 loci to be associated with peduncle length (PL), ray floret length (RFL), and outer ray floret; the level of apex relative to the top of involucre (LAI); outer corolla lips (OCL); and the b* of ray floret color, respectively. Our results reveal the genetic patterns of important ornamental traits and provide a theoretical basis and practical tools for gerbera genetic breeding.
Exposure to Cry1 Toxins Increases Long Flight Tendency in Susceptible but Not in Cry1F-Resistant Female Spodoptera frugiperda (Lepidoptera: Noctuidae)
Authors: De Bortoli Caroline P. , Santos Rafael F. , Assirati Giordano J. , Sun Xiaocun , Hietala Lucas , Jurat-Fuentes Juan Luis
Published: December 2023

The fall armyworm (JE Smith) (Spodoptera frugiperda) is a polyphagous pest targeted by selected Cry and Vip3A insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) that are produced in transgenic Bt corn and cotton. Available evidence suggests that sublethal larval exposure to Cry1Ac increases flight activity in adult Spodoptera spp. However, it is not known whether this effect is also observed in survivors from generally lethal exposure to Cry1Ac. Moreover, while multiple cases of field-evolved resistance to Bt proteins have been described in the native range of S. frugiperda, the effect of resistance on flight behavior has not been examined. Long-distance migratory flight capacity of S. frugiperda is of concern given its ongoing global spread and the possibility that migrants may be carrying resistance alleles against pesticides and Bt crops. In this study, we used rotational flight mills to test the effects of generally lethal exposure to Cry1Ac in susceptible and sublethal exposure in Cry1F-resistant S. frugiperda strains. The results detected altered pupal weight after larval feeding on diet containing Cry proteins, which only translated in significantly increased tendency for longer flights in female moths from the susceptible strain. This information has relevant implications when considering current models and assumptions for resistance management of Bt crops.
Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals
Authors: Dinić Ivana , Vuković Marina , Rabanal Maria Eugenia , Milošević Milica , Bukumira Marta , Tomić Nina , Tomić Miloš , Mančić Lidija , Ignjatović Nenad
Published: December 2023

Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.
Effects of the Wall Temperature on Rarefied Gas Flows and Heat Transfer in a Micro-Nozzle
Authors: Zhang Shurui , Li Yong , Wang Xudong , Lu Songcai , Yu Yusong , Yang Jun
Published: December 2023

When the satellite is in orbit, the thruster will experience drastic temperature changes (100–1000 K) under solar radiation, which will affect the rarefied gas flow state in the micro-nozzle structure of the cold gas micro-thruster. In this study, the effect of different wall temperatures on the rarefied flow and heat transfer in the micro-nozzle is investigated based on the DSMC method. The micro-nozzle structure in this paper has a micro-channel with a large length-to-diameter ratio of 10 and a micro-scale needle valve displacement (maximum needle valve displacement up to 4 μm). This leads to more pronounced multiscale flow characteristics in the micro-nozzle, which is more influenced by the change in wall temperature. At wall temperatures ranging from 100 K to 1000 K, the spatial distribution of local Kn distribution, slip velocity distribution, temperature, and wall heat flux distribution in the micro-nozzle were calculated. The slip flow region is located in the flow channel and transforms into transition flow as the slip velocity reaches approximately 50 m/s. The spatial distribution of the flow pattern is dominated by the wall temperature at small needle valve opening ratios. The higher the wall temperature, the smaller the temperature drop ratio in the low-temperature region inside the micro-nozzle. The results of the study provide a reference for the design of temperature control of micro-nozzles in cold gas micro-thrusters.
Dynamic Light Scattering and Its Application to Control Nanoparticle Aggregation in Colloidal Systems: A Review
Authors: Rodriguez-Loya Jesus , Lerma Maricarmen , Gardea-Torresdey Jorge L.
Published: December 2023

Colloidal systems and their control play an essential role in daily human activities, but several drawbacks lead to an avoidance of their extensive application in some more productive areas. Some roadblocks are a lack of knowledge regarding how to influence and address colloidal forces, as well as a lack of practical devices to understand these systems. This review focuses on applying dynamic light scattering (DLS) as a powerful tool for monitoring and characterizing nanoparticle aggregation dynamics. We started by outlining the core ideas behind DLS and how it may be used to examine colloidal particle size distribution and aggregation dynamics; then, in the last section, we included the options to control aggregation in the chemically processed toner. In addition, we pinpointed knowledge gaps and difficulties that obstruct the use of DLS in real-world situations. Although widely used, DLS has limits when dealing with complicated systems, including combinations of nanoparticles, high concentrations, and non-spherical particles. We discussed these issues and offered possible solutions and the incorporation of supplementary characterization approaches. Finally, we emphasized how critical it is to close the gap between fundamental studies of nanoparticle aggregation and their translation into real-world applications, recognizing challenges in colloidal science.
Unveiling the Synergy of Coupled Gold Nanoparticles and J-Aggregates in Plexcitonic Systems for Enhanced Photochemical Applications
Authors: Jumbo-Nogales Alba , Rao Anish , Olejniczak Adam , Grzelczak Marek , Rakovich Yury
Published: December 2023

Plexcitonic systems based on metal nanostructures and molecular J-aggregates offer an excellent opportunity to explore the intriguing interplay between plasmonic excitations and excitons, offering unique insights into light–matter interactions at the nanoscale. Their potential applications in photocatalysis have prompted a growing interest in both their synthesis and the analysis of their properties. However, in order to construct a high-performing system, it is essential to ensure chemical and spectral compatibility between both components. We present the results of a study into a hybrid system, achieved through the coupling of gold nanobipyramids with organic molecules, and demonstrate the strengthened photochemical properties of such a system in comparison with purely J-aggregates. Our analysis includes the absorbance and photoluminescence characterization of the system, revealing the remarkable plexcitonic interaction and pronounced coupling effect. The absorbance spectroscopy of the hybrid systems enabled the investigation of the coupling strength (g). Additionally, the photoluminescence response of the J-aggregates and coupled systems reveals the impact of the coupling regime. Utilizing fluorescence lifetime imaging microscopy, we established how the photoluminescence lifetime components of the J-aggregates are affected within the plexcitonic system. Finally, to assess the photodegradation of J-aggregates and plexcitonic systems, we conducted a comparative analysis. Our findings reveal that plasmon-enhanced interactions lead to improved photostability in hybrid systems.
Nanotechnologies and Nanomaterials: Selected Papers from CCMR
Authors: Li Ming-Yu , Lee Jihoon
Published: December 2023

Nanomaterial technology for the synthesis, processing, and fabrication of low-dimensional materials is where disciplines merge into a remarkable range of applications, from optoelectronics to health care (contribution 1–7), which affect the lives of millions [...]
Precipitation Stripping of V(V) as a Novel Approach for the Preparation of Two-Dimensional Transition Metal Vanadates
Authors: Sánchez-Loredo María Guadalupe , Chekhonin Paul , Ebert Doreen , Fischer Ulrike , Liu Xu , Möckel Robert , Labrada-Delgado Gladis Judith , Passerini Stefano , Kelly Norman
Published: December 2023

Cobalt, nickel, manganese and zinc vanadates were synthesized by a hydrometallurgical two-phase method. The extraction of vanadium (V) ions from alkaline solution using Aliquat® 336 was followed by the production of metal vanadates through precipitation stripping. Precipitation stripping was carried out using solutions of the corresponding metal ions (Ni (II), Co (II), Mn (II) and Zn (II), 0.05 mol/L in 4 mol/L NaCl), and the addition time of the strip solution was varied (0, 1 and 2 h). The time-dependent experiments showed a notable influence on the composition, structure, morphology and crystallinity of the two-dimensional vanadate products. Inspired by these findings, we selected two metallic vanadate products and studied their properties as alternative cathode materials for nonaqueous sodium and lithium metal batteries.
Body Mass Index Trends before and during the COVID-19 Pandemic in Primary School Students in Split-Dalmatia County, Croatia: A Retrospective Study
Authors: Androja Luka , Bavčević Tonči , Jurčev Savičević Anamarija , Bavčević Damir , Ninčević Jasna , Buljan Anita , Nonković Diana , Rodrigues Vitor , Karin Željka
Published: December 2023

Within the last decade, childhood obesity has become a serious problem, especially during the COVID-19 pandemic. This research paper aimed to examine whether body mass index (BMI) was higher during the pandemic (2020–2022) than in the pre-pandemic period (2012/2013–2019) using trends related to sex, urban–rural area, and physical activity (PA). This study included data from physical examinations of an entire population of primary school children from Split-Dalmatia County (Croatia) over a period of 10 years. There were 103,804 students from the first, fifth, and eighth grades who participated in the analysis. During the pandemic, the BMI of all the students increased, except for eighth-grade girls. Generations of eighth graders have had significantly different BMIs throughout the past decade. We found that first graders were overweight and obese in urban areas, while in rural areas, this problem was experienced by upper grades. Girls were more overweight and obese in the first and eighth grades, but boys experienced this more in the fifth grade. Reduced PA influenced an increase in BMI in both sexes, especially in girls. During the pandemic, this situation became worse. This study could be useful to experts for the creation of new policies for Split-Dalmatia County and surrounding regions that are similar economically and culturally.
The Remodulation of Actin Bundles during the Stimulation of Mitochondria in Adult Human Fibroblasts in Response to Light
Authors: Olejárová Soňa , Horváth Denis , Huntošová Veronika
Published: December 2023

β-actin belongs to cytoskeletal structures that change dynamically in cells according to various stimuli. Human skin can be considered as an organ that is very frequently exposed to various stress factors, of which light plays an important role. The present study focuses on adult human fibroblasts exposed to two types of light stress. Orange light with a wavelength of 590 nm was used here to stimulate the photosensitizer localized in the cells as a residual dose of photodynamic therapy (PDT). On the other hand, near-infrared light with a wavelength of 808 nm was considered for photobiomodulation (PBM), which is often used in healing processes. Confocal fluorescence microscopy was used to observe changes in intercellular communication, mitochondrial structures, and cytoskeletal dynamics defined by the remodulation of β-actin of fibroblasts. The number of β-actin bundles forming spherical structures was detected after light exposure. These structures as β-actin oligomers were confirmed with super-resolution microscopy. While PDT led to the disintegration of actin oligomers, PBM increased their number. The interaction of β-actin with mitochondria was observed. The combination of PDT and PBM treatments is important to minimize the side effects of cancer treatment with PDT on healthy cells, as shown by the cell metabolism assay in this work. In this work, β-actin is presented as an important parameter that changes and is involved in the response of cells to PDT and PBM.
Remote Activation of Mechanotransduction via Integrin Alpha-5 via Aptamer-Conjugated Magnetic Nanoparticles Promotes Osteogenesis
Authors: Hajiali Hadi , Rotherham Michael , El Haj Alicia J.
Published: December 2023

Bone regeneration and repair are complex processes in the adult skeleton, and current research has focused on understanding and controlling these processes. Magnetic nanoparticle (MNP)-based platforms have shown potential in tissue engineering and regenerative medicine through the use of magnetic nanomaterials combined with remotely applied dynamic fields. Previous studies have demonstrated the ability of MNP-induced mechanoactivation to trigger downstream signaling and promote new bone formation. In this study, we aimed to compare the osteogenic induction achieved using the mechanoreceptor targets, Piezo1, Fzd1, Fzd2, and integrin alpha-5. We compared the binding efficacy of different types of agonists (antibodies vs. aptamers) to these receptors. Moreover, we optimized the aptamer concentration (2.5, 5, and 10 μg/mg) for the selected receptor to determine the optimum concentration for promoting bone formation. Our data demonstrated that the mechanoactivation of integrins (CD49e) significantly upregulated the RUNX2 and LEF1 genes compared to other selected receptors. Furthermore, comparing the mechanoactivation of cells using MNPs conjugated with CD49e antibodies and aptamers revealed that MNP–aptamers significantly enhanced the upregulation of LEF1 genes. This suggests that aptamer-mediated mechanoactivation is a promising alternative to antibody-mediated activation. Finally, our results showed that the concentration of the aptamer loaded onto the MNPs strongly influenced the mechanoactivation of the cells. These findings provide valuable insights into the use of MNP platforms for bone regeneration and highlight the potential of aptamers in promoting signaling pathways related to bone formation. The novelty of our study lies in elucidating the unique advantages of aptamers in mediating mechanoactivation, presenting a promising avenue for advancing bone regenerative strategies.
Using Date Palm Waste as an Alternative for Rockwool: Sweet Pepper Performance under Both Soilless Culture Substrates
Authors: Qaryouti Muein , Osman Mohamed , Alharbi Abdulaziz , Voogt Wim , Abdelaziz Mohamed Ewis
Published: December 2023

The degradation of soil quality due to environmental conditions and improper management practices has caused a shrinkage in land areas suitable for crop cultivation. This necessitates a transition towards soilless culture systems, which offer desirable conditions for crop growth and development and increase resource use efficiency. One of the growth-limiting factors in soilless culture systems is the type of growing substrate. The use of more sustainable resources and environmentally friendly growing substrates is a challenge that affects the soilless culture industry. This work evaluates the efficacy of date palm waste (DPW) and rockwool as growing substrates for sweet pepper (Capsicum annuum L.) under greenhouse conditions. The plant height, stem diameter, average total leaf area, φPSII, and Fm′ of leaf fluorescence show significant increases when plants are grown in rockwool. No differences are found in terms of the total yield or the number of marketable fruits and fruit quality between the two substrates. However, the DPW substrate shows a significant decrease in the number of unmarketable fruits and number of Blossom End Rot (BER) fruits. Plants grown in both growing substrates consume equal water amounts for the optimal fruit production, while the water use efficiency of rockwool is better than that of DPW. Our results highlight DPW’s role in soilless production and as a key solution for resource-saving production systems.
Consolidation of Calcium Carbonate Using Polyacrylamides with Different Chemistries
Authors: Lew Jin Hau , Luckham Paul F. , Matar Omar K. , Müller Erich A. , Santos Adrielle Sousa , Maung Maung Myo Thant
Published: December 2023

In this work, the consolidation of calcium carbonate (CaCO3) by polyacrylamide (PAM) of different molecular weights, charge densities, and functional groups was investigated via oscillatory rheology and unconfined compressive strength (UCS) analysis. Oscillatory rheology showed that the storage modulus G′ was approximately 10 times higher than the loss modulus G″, indicating a highly elastic CaCO3 sample upon consolidation via PAM. Both oscillatory rheology and UCS analysis exhibited similar trends, wherein the mechanical values (G′, G″, and UCS) first increased with increasing polymer dosage, until they reached a peak value (typically at 3 mgpol/gCaCO3), followed by a decrease in the mechanical values. This indicates that there is an optimum polymer dosage for the different PAM-CaCO3 colloidal systems, and that exceeding this value induces the re-stabilisation of the colloidal system, leading to a decreased degree of consolidation. Regarding the effect of the PAM molecular weight, the peak G′ and UCS values of CaCO3 consolidated by hydrolysed PAM (HPAM) of different molecular weights are very similar. This is likely due to the contour length of the HPAMs being either almost the same or longer than the average distance between two CaCO3 particles. The effect of the PAM charge density revealed that the peak G′ and UCS values decreased as the charge density of the PAM increased, while the optimum PAM dosage increased with decreasing PAM charge density. The higher likelihood of lower-charge PAM bridging between the particles contributes to higher elastic energy and mechanical strength. Finally, regarding the PAM functional group, CaCO3 consolidated by sulfonated polyacrylamide (SPAM) typically offers lower mechanical strength than that consolidated with HPAM. The bulky sulfonate side groups of SPAM interfere with the surface packing, reducing the number of polymers able to adsorb onto the surface and, eventually, reducing the degree of consolidation of CaCO3. The zeta potential of the PAM-CaCO3 samples became more negative with increasing PAM concentration due to the saturation of the particle surface. Good agreement between oscillatory rheology and UCS analysis could accelerate PAM screening for optimum CaCO3 consolidation.
A Libertarian Anarchist Analysis of Norman Geisler’s Philosophy of Government
Authors: Miller Anthony Michael
Published: December 2023

There are numerous approaches and conclusions regarding church and state relations and how Christianity affects public policy. Yet the purpose of this study is to question some of the philosophical assumptions and biblical interpretations that Christians hold to which support the state as a morally legitimate authoritative institution in the first place. This article will argue that various presuppositions regarding the state’s moral legitimacy are untenable, if not self-refuting. The philosophical commitments of a form of Christian Conservatism exemplified by Norman L. Geisler will be analyzed and critiqued by the Christian Libertarian Anarchist school of thought, represented by Gerard Casey. Geisler’s views on first principles, God’s moral law, social contracts, consent, anarchy, the distinction between vices and crimes, preconditions for virtue, and the common good will be examined. Then, Geisler’s interpretation of classic biblical texts supporting the alleged moral legitimacy of the state will also be assessed. This article will contend that if one were to consistently apply some pertinent principles found in Geisler’s prolegomena to theology when reasoning from natural revelation and the relevant biblical data, one will find that the conclusions are more compatible with the political theology of Christian Libertarian Anarchism. Hence the one who questions how Christianity affects public policy should take into consideration the reasons to deny that divine revelation affirms the state as a morally legitimate authoritative institution. If this is the case, the question ought to be reframed to determine how Christianity affects public policy within a state that has no legitimate moral grounds for authority.
Renal and Urinary Tract Involvement in Fibrosclerosing or Fibroinflammatory Diseases: A Narrative Review
Authors: Rossi Giovanni Maria , Pala Chiara , Gianfreda Davide
Published: December 2023

Fibroinflammatory diseases are a group of rare pathologies in which the hallmark is the exuberant deposition of fibrotic tissue and inflammatory cellular infiltrates, characteristic of the specific disease. A sclerotic mass develops within soft tissues and/or organs, damaging and replacing them, with effects ranging from asymptomatic to life-threatening clinical manifestations. The kidneys and urinary tract can be involved in some of these diseases, which can lead to acute kidney injury, chronic kidney disease, and even end-stage kidney disease. IgG4-related disease, retroperitoneal fibrosis, and Erdheim–Chester disease are the three fibroinflammatory disorders that can involve the kidneys. Only a timely and accurate collection of clinical, radiological, metabolic, laboratory, and histological data allows prompt diagnosis and targeted treatment of these pathologies, allowing the stoppage of the evolution of renal and systemic manifestations, which can lead to complete remission. The epidemiology, clinical and histological features, and management of these conditions are herein described in a narrative fashion.
Trend Classification of InSAR Displacement Time Series Using SAE–CNN
Authors: Li Menghua , Wu Hanfei , Yang Mengshi , Huang Cheng , Tang Bo-Hui
Published: December 2023

Multi-temporal Interferometric Synthetic Aperture Radar technique (MTInSAR) has emerged as a valuable tool for measuring ground motion in a wide area. However, interpreting displacement time series and identifying dangerous signals from millions of InSAR coherent targets is challenging. In this study, we propose a method combining stacked autoencoder (SAE) and convolutional neural network (CNN) to classify InSAR time series and ease the interpretation of movements. The InSAR time series are classified into five categories, including stable, linear, accelerating, deceleration, and phase unwrapping error (PUE). The accuracy of labeled samples reaches 95.1%, reflecting the performance of the proposed method. This method was applied to the InSAR results for Kunming extracted from 171 ascending Sentinel-1 images from January 2017 to September 2022. The classification map of the InSAR time series shows that stable coherent points dominate around 79.28% of the area, with linear patterns at 10.70%, decelerating at 5.30%, accelerating at 4.72%, and PUE patterns at 3.60%. The results demonstrate that this method can distinguish different ground motion features and detect nonlinear deformation signals on a large scale without human intervention.
Surface Subsidence over a Coastal City Using SBAS-InSAR with Sentinel-1A Data: A Case of Nansha District, China
Authors: Yu Huanghao , Li Binquan , Xiao Yang , Sun Jinyan , Chen Cheng , Jin Gaoyang , Liu Huanyu
Published: December 2023

The loss of life and property in economically developed areas due to geological hazards caused by intense ground subsidence is incalculable. As one of the fastest growing areas in the Guangdong-Hong Kong-Macao Greater Bay Area, the study of ground subsidence in Nansha will help to provide a scientific basis for urban planning and improve the capacity of monitoring and prevention of ground subsidence. The combination of coastal soft soil foundation and urbanization conditions creates a certain risk of land subsidence. We chose Nansha District, the geographical center of the Greater Bay Area, as the study area to analyze its surface subsidence characteristics in recent years. The 20-view Sentinel-1A data and SBAS-InSAR technique were used to monitor the ground subsidence in Nansha from 2017 to 2023. The main rate of ground subsidence in Nansha ranges from −19.4 to 7.7 mm/yr and is distributed in the urban area, along the rivers, in the construction area, and in the reclamation area. As of 4 May 2023, the average ground settlement in Nansha is 10.05 mm and the maximum settlement can be up to 142.45 mm. The 6-year total settlement at all four settlement intensities is greater than 60 mm, with the highest value exceeding 110 mm. The cumulative settlement increases with time, but inverse settlement and no settlement also occur at points where settlement is severe. For settlement caused by soft soil consolidation, it is recommended that drainage pipes be installed to accelerate drainage as a means of stabilizing settlement. For settlement caused by groundwater extraction and additional loads on the road surface, it is recommended to rationally extract groundwater and reinforce the foundation of the road surface with severe settlement.
A Terrestrial Laser Scanning-Based Method for Indoor Geometric Quality Measurement
Authors: Tan Yi , Liu Xin , Jin Shuaishuai , Wang Qian , Wang Daochu , Xie Xiaofeng
Published: December 2023

The indoor geometric dimensions of a building are crucial for acceptance criteria. Traditional manual methods for measuring indoor geometric quality are labor-intensive, time-consuming, error-prone, and yield non-reproducible results. With the advancement of ground-based laser scanning technology, the efficient and precise measurement of geometric dimensions has become achievable. An indoor geometric quality measurement method based on ground-based laser scanning is presented in this paper. Initially, a coordinate transformation algorithm based on selected points was developed for conducting coordinate conversion. Subsequently, the Cube Diagonal-based Denoising algorithm, developed for point cloud denoising, was employed. Following that, architectural components such as walls, ceilings, floors, and openings were identified and extracted based on their spatial relationships. The measurement and visualization of the geometric quality of walls’ flatness, verticality, and opening dimensions were automated using fitting and simulation methods. Lastly, tests and validation were conducted to assess the accuracy and applicability of the proposed method. The experimental results demonstrate that time and human resources can be significantly saved using this method. The accuracy of this method in assessing wall flatness, verticality, and opening dimensions is 77.8%, 88.9%, and 95.9%, respectively. These results indicate that indoor geometric quality can be detected more accurately and efficiently compared to traditional inspection methods using the proposed method.
Enhanced Practical Byzantine Fault Tolerance via Dynamic Hierarchy Management and Location-Based Clustering
Authors: Kim Gwangyong , Cho Jinsung , Choi Min , Kim Bongjae
Published: December 2023

Blockchain is a distributed database technology that operates in a P2P network and is used in various domains. Depending on its structure, blockchain can be classified into types such as public and private. A consensus algorithm is essential in blockchain, and various consensus algorithms have been applied. In particular, a non-competitive consensus algorithm called PBFT is mainly used in private blockchains. However, there are limitations to scalability. This paper proposes an enhanced PBFT with dynamic hierarchy management and location-based clustering to overcome these problems. The proposed method clusters nodes based on location information and adjusts the dynamic hierarchy to optimize consensus latency. As a result of the experiment, the proposed PBFT showed significant performance improvement compared to the existing typical PBFT and Dynamic Layer Management PBFT (DLM-PBFT). The proposed PBFT method showed a processing performance improvement rate of approximately 107% to 128% compared to PBFT, and 11% to 99% compared to DLM-PBFT.
Experimental Study of Fully Passive, Fully Active, and Active–Passive Upper-Limb Exoskeleton Efficiency: An Assessment of Lifting Tasks
Authors: Nasr Ali , Dickerson Clark R. , McPhee John
Published: December 2023

Recently, robotic exoskeletons are gaining attention for assisting industrial workers. The exoskeleton power source ranges from fully passive (FP) to fully active (FA), or a mixture of both. The objective of this experimental study was to assess the efficiency of a new active–passive (AP) shoulder exoskeleton using statistical analyses of 11 quantitative measures from surface electromyography (sEMG) and kinematic data and a user survey for weight lifting tasks. Two groups of females and males lifted heavy kettlebells, while a shoulder exoskeleton helped them in modes of fully passive (FP), fully active (FA), and active–passive (AP). The AP exoskeleton outperformed the FP and FA exoskeletons because the participants could hold the weighted object for nearly twice as long before fatigue occurred. Future developments should concentrate on developing sex-specific controllers as well as on better-fitting wearable devices for women.
Sonification for Personalised Gait Intervention
Authors: Wall Conor , McMeekin Peter , Walker Richard , Hetherington Victoria , Graham Lisa , Godfrey Alan
Published: December 2023

Mobility challenges threaten physical independence and good quality of life. Often, mobility can be improved through gait rehabilitation and specifically the use of cueing through prescribed auditory, visual, and/or tactile cues. Each has shown use to rectify abnormal gait patterns, improving mobility. Yet, a limitation remains, i.e., long-term engagement with cueing modalities. A paradigm shift towards personalised cueing approaches, considering an individual’s unique physiological condition, may bring a contemporary approach to ensure longitudinal and continuous engagement. Sonification could be a useful auditory cueing technique when integrated within personalised approaches to gait rehabilitation systems. Previously, sonification demonstrated encouraging results, notably in reducing freezing-of-gait, mitigating spatial variability, and bolstering gait consistency in people with Parkinson’s disease (PD). Specifically, sonification through the manipulation of acoustic features paired with the application of advanced audio processing techniques (e.g., time-stretching) enable auditory cueing interventions to be tailored and enhanced. These methods used in conjunction optimize gait characteristics and subsequently improve mobility, enhancing the effectiveness of the intervention. The aim of this narrative review is to further understand and unlock the potential of sonification as a pivotal tool in auditory cueing for gait rehabilitation, while highlighting that continued clinical research is needed to ensure comfort and desirability of use.
A Game-Based Computing Resource Allocation Scheme of Edge Server in Vehicular Edge Computing Networks Considering Diverse Task Offloading Modes
Authors: Liu Xiangyan , Zheng Jianhong , Zhang Meng , Li Yang , Wang Rui , He Yun
Published: December 2023

Introducing partial task offloading into vehicle edge computing networks (VECNs) can ease the burden placed on the Internet of Vehicles (IoV) by emerging vehicle applications and services. In this circumstance, the task offloading ratio and the resource allocation of edge servers (ES) need to be addressed urgently. Based on this, we propose a best response-based centralized multi-TaV computation resource allocation algorithm (BR-CMCRA) by jointly considering service vehicle (SeV) selection, offloading strategy making, and computing resource allocation in a multiple task vehicle (TaV) system, and the utility function is related to the processing delay of all tasks, which ensures the TaVs’s quality of services (QoS). In the scheme, SeV is first selected from three candidate SeVs (CSVs) near the corresponding TaV based on the channel gain. Then, an exact potential game (EPG) is conducted to allocate computation resources, where the computing resources can be allocated step by step to achieve the maximum benefit. After the resource allocation, the task offloading ratio can be acquired accordingly. Simulation results show that the proposed algorithm has better performance than other basic algorithms.
Flood Inundation and Streamflow Changes in the Kabul River Basin under Climate Change
Authors: Baig Sohaib , Hasson Shabeh ul
Published: December 2023

The Kabul basin yields around 16% of the total annual water availability in Pakistan. Changing climate will alter the precipitation regime in terms of intensity and frequency, which will affect the water yield and cause flood hazards. Against this background, this study aims to quantify the impacts of changing climate on the water yield, its timings, and, more importantly, the associated flood hazards in the transboundary Kabul basin. For this, we used a rainfall-runoff inundation (RRI) model coupled with the snow and glacier melt routines and drove it for historical and future climates simulated by the atmosphere-only general circulation model (AGCM) at 20 km spatial resolution. The model simulations reveal that rainfall runoff contributes around 50% of the annual flows, and the rest is contributed by glaciers and snow melts. Annual precipitation is projected to increase by 14% from 535 mm, whereas temperatures will rise by 4.7 °C. In turn, the Kabul River flows will only increase by 4% to 1158 m3s−1 from 1117 m3s−1, mainly due to an increase in winter flows. In contrast to a minute increase in the mean river flows, the maximum flood inundation area is projected to increase by 37%, whereas its depth will rise between 5 and 20 cm.
CNN with New Spatial Pyramid Pooling and Advanced Filter-Based Techniques: Revolutionizing Traffic Monitoring via Aerial Images
Authors: Javid Irfan , Ghazali Rozaida , Saeed Waddah , Batool Tuba , Al-Wajih Ebrahim
Published: December 2023

The escalation in vehicular traffic, in conjunction with global population growth, has precipitated heightened road congestion, augmented air pollution, and a rise in vehicular accidents. Over the past decade, the global vehicular count has witnessed a substantial surge. In this context, traffic monitoring emerges as a paramount challenge, especially within developing nations. This research introduces an innovative system for vehicle detection and categorization aimed at intelligent traffic monitoring. The system utilizes a convolutional neural network-based U-Net model for the segmentation of aerial images. After segmentation, the outputs are further analyzed for vehicle identification. This vehicle detection utilizes an advanced spatial pyramid pooling (ASPP) mechanism which refines the spatial partitions of the image and captures intricate details, enhancing the accuracy and precision of the detection process. Detected vehicles are then categorized into distinct subcategories. For the effective management and control of high-density traffic flow, the extended Kalman filter (EKF) technique is employed, thereby reducing the reliance on human oversight. In experimental evaluations, our proposed model exhibits exemplary vehicle detection capabilities across the German Aerospace Center (DLR3K) and the Vehicle Detection in Aerial Imagery (VEDAI) datasets. Potential applications of the system encompass vehicle identification in traffic streams, traffic congestion assessment, intersection traffic density analysis, differentiation of vehicle types, and pedestrian pathway determination.
Visual Analysis to Assess Attraction and Organisation of Contemporary Metropolitan Systems—A Case Study of Central and Northern Italy
Authors: Ganciu Amedeo , Balestrieri Mara
Published: December 2023

The landscape of scientific research is rich with experiments aimed at identifying polycentric morphologies, defining their degree of polycentricity, and the socio-economic and environmental relationships that develop within them. However, some aspects are still under-researched, such as defining a procedure for determining the extent of the metropolitan area of influence. This research aims to experiment with a graphical–analytical methodology aimed at identifying and representing the functional area of metropolises, i.e., the territorial limit beyond which a metropolis’ attractiveness ceases to exert its territorial influence, and which also allows the territorial ramification of urban cores with greater attractiveness to be determined and visualized graphically. Using Visual Analytics as a conceptual basis, it is possible to combine methods and technologies that harness the potential of human understanding with the increased capabilities of electronic data processing for a more adequate understanding of the research scope. For this research, the graph-analytic mix adopted comprises the graph theory algorithms for the analytical quantification of relationships and the reference surface area of polycentric metropolises. In contrast, the subsequent visualisation of relationships and their spatial branching is based on the electronic evolution of graphical techniques based on the works of Henry Drury Harness and Charles Joseph Minard, particularly those invented to map commuter flows and migrations. The research results, focusing on Northern Italy, demonstrate a highly interconnected and polycentric system, with macro-areas whose functional boundaries seldom coincide with the administrative boundaries of the regions. The research demonstrates the existence of five territorial macro-regions encompassing the 11 regions outlined in the Italian state’s legislation, containing polycentric metropolitan systems with distributional characteristics that differ from each other.