bluuebunny/crossref_metadata_2025 huggingface dataset:
Hybrid plaintext attack for a cryptosystem based on interference and the phase-retrieval technique
Authors: Xiong Y. , Gu J. , Kumar R.
Published: May 2023

In this paper, the security strength of an enhanced cryptosystem based on interference and the phase-retrieval technique is evaluated. The security strength of the optical cryptosystem was improved through the phase-retrieval technique used to generate a phase-only mask (POM) as the ciphertext. Due to the complex mathematical model of the phase-retrieval technique, it seems that a silhouette problem existing in the conventional interference-based scheme was removed. However, we noted that the random phase mask (RPM) regarded as the only private key was fixed in the encryption path, which is not related to the plaintext and makes it possible to be recovered using a known-plaintext attack (KPA). Moreover, we also found that the RPM has high key sensitivity, and it should be recovered precisely to retrieve information of plaintexts during the attack. Thus, a hybrid KPA where three pairs of known plaintexts and their ciphertexts are regarded as the amplitude and phase constraints to obtain the precise estimation of the RPM is proposed. Then, with the help of the estimated private key, information of the original plaintexts encoded using the cryptosystem under study could be retrieved from an arbitrarily given ciphertext without any knowledge of the private key. Our cryptoanalysis shows that the cryptosystem based on interference and the phase-retrieval technique is vulnerable to the proposed attack, and there is a security leak in it. Numerical simulations have been carried out to demonstrate the performance of our proposed attack.
Terahertz Bessel beam generator
Authors: Li Jiu-Sheng , Cheng Jie , Zhang Dong-Ping
Published: May 2023

The Bessel beam has broad application prospects in wireless energy transmission and high-speed communications. The traditional Bessel beam generation method has the problems of large volume, low efficiency, and complex manufacturing. To solve the above problems, we present a terahertz Bessel beam generator based on the reflective metasurface, which is composed of a metal pattern, dielectric layer, and bottom metal plate. Under the incidence of right circularly polarized (RCP) wave, the zero-order Bessel beam and zero-order symmetric double Bessel beam are generated. It can be found that the bottom angle of the axicon of the first-order Bessel beam is inversely proportional to the propagation distance of the Bessel beam. Comparing the electric field intensity distribution, phase distribution, and mode purity of the second-order Bessel beam and the second-order vortex beam in different observation planes, it can be seen that the energy of the higher-order Bessel beam is more concentrated and the field distribution is more stable than those of the ordinary vortex beam. The reflective terahertz Bessel beam generator has potential application value in terahertz wireless communications, measurement, radar detection, and imaging.
THz Bragg structures fabricated with additive manufacturing
Authors: Brodie C. Harrison , Spotts Isaac , Collier Christopher M.
Published: May 2023

The advancement of THz science and technology is desirable to
facilitate the application of THz technologies in many sectors.
Specialized THz photonic elements for these applications require
desirable absorption and refractive characteristics in the THz regime.
THz photonic elements can be created with additive manufacturing, and
specifically 3D printing, forgoing the need for complex fabrication
procedures and methodologies. Such THz photonic elements include
periodic Bragg structures, which are capable of filtering specific THz
frequencies. The authors present a THz Bragg structure fabricated with
3D printing via fused filament fabrication. The THz Bragg structure is
made from high-impact polystyrene filament material, which is
characterized in this paper with THz time-domain spectroscopy. The
geometry and theoretical operation of the THz Bragg structure is
investigated with finite-difference time-domain electromagnetic
simulations. The THz Bragg structure is evaluated using a THz
experimental test bed. There is agreement between the theoretical and
the experimental filtering placement within the frequency domain for
the THz Bragg structure. The capability of tunable frequency filtering
of the presented THz Bragg structure, fabricated with 3D printing, is
established and facilitates future advancements in applications of THz
science and technology.
One-shot reflectance direction field imaging for measuring the surface slope distribution of a capillary wave
Authors: Ohno Hiroshi , Usui Takashi
Published: May 2023

A method for measuring a surface slope distribution of a capillary wave is proposed. The method uses an optical imaging system that can capture a one-shot image of a light-reflectance direction field in a two-dimensional image plane. A dispersion relation between the wavelength and frequency of the capillary wave is shown to be obtainable by the imaging system, which agrees well with the theoretical prediction.
Deep learning for laser beam imprinting
Authors: Chalupský J. , Vozda V. , Hering J. , Kybic J. , Burian T. , Dziarzhytski S. , Frantálová K. , Hájková V. , Jelínek Š. , Juha L. , Keitel B. , Kuglerová Z. , Kuhlmann M. , Petryshak B. , Ruiz-Lopez M. , Vyšín L. , Wodzinski T. , Plönjes E.
Published: May 2023

Methods of ablation imprints in solid targets are widely used to characterize focused X-ray laser beams due to a remarkable dynamic range and resolving power. A detailed description of intense beam profiles is especially important in high-energy-density physics aiming at nonlinear phenomena. Complex interaction experiments require an enormous number of imprints to be created under all desired conditions making the analysis demanding and requiring a huge amount of human work. Here, for the first time, we present ablation imprinting methods assisted by deep learning approaches. Employing a multi-layer convolutional neural network (U-Net) trained on thousands of manually annotated ablation imprints in poly(methyl methacrylate), we characterize a focused beam of beamline FL24/FLASH2 at the Free-electron laser in Hamburg. The performance of the neural network is subject to a thorough benchmark test and comparison with experienced human analysts. Methods presented in this Paper pave the way towards a virtual analyst automatically processing experimental data from start to end.
Improvement of optical properties of InGaN-based red multiple quantum wells
Authors: Hou Xin , Yang Tao , Fan Shao-Sheng , Xu Huan , Iida Daisuke , Liu Yue-Jun , Mei Yang , Weng Guo-En , Chen Shao-Qiang , Zhang Bao-Ping , Ohkawa Kazuhiro
Published: May 2023

The realization of red-emitting InGaN quantum well (QW) is a hot issue in current nitride semiconductor research. It has been shown that using a low-Indium (In)-content pre-well layer is an effective method to improve the crystal quality of red QWs. On the other hand, keeping uniform composition distribution at higher In content in red QWs is an urgent problem to be solved. In this work, the optical properties of blue pre-QW and red QWs with different well width and growth conditions are investigated by photoluminescence (PL). The results prove that the higher-In-content blue pre-QW is beneficial to effectively relieve the residual stress. Meanwhile, higher growth temperature and growth rate can improve the uniformity of In content and the crystal quality of red QWs, enhancing the PL emission intensity. Possible physical process of stress evolution and the model of In fluctuation in the subsequent red QW are discussed. This study provides a useful reference for the development of InGaN-based red emission materials and devices.
Functionally integrated waveguide illuminator for common-path digital holographic microscopy through random media
Authors: Iida Kohei , Hayashi Kenta , Faheem Maryam , Okamoto Katsunari , Takeda Mitsuo , Watanabe Eriko
Published: May 2023

We designed and fabricated a functionally integrated optical waveguide illuminator specially for common-path digital holographic microscopy through random media. The waveguide illuminator creates two point sources with desired phase shifts, which are located close to one another so that the common-path condition of the object and reference illumination is satisfied. Thereby, the proposed device permits phase-shift digital holographic microscopy free from bulky optical elements such as a beam splitter, an objective lens, and a piezoelectric transducer for phase shifting. Using the proposed device, microscopic 3D imaging through a highly heterogeneous double-composite random medium was experimentally demonstrated by means of common-path phase-shift digital holography.
Highly sensitive quasi-D-shaped photonic crystal fiber biosensor designed for the detection of RBC parasitized by Plasmodium falciparum for the early diagnosis of malaria
Authors: Nijhum Jannatul Mawa , Ahmed Tanvir
Published: May 2023

A simple quasi-D-shaped photonic crystal fiber (PCF)-based surface plasmon resonance biosensor is proposed for the early diagnosis of malaria that arises as a result of Plasmodium falciparum parasite development in erythrocytes in the human body. The flat surface of the D-shaped PCF is covered with a thin layer of TiO2 along with a gold layer. The finite element method (FEM) is used to numerically investigate the characteristics of the sensor. With the well-optimized set of parameters, the proposed sensor exhibits maximum spectral sensitivities of 42857.14 nm/RIU, 22105.26 nm/RIU, and 16206.90 nm/RIU with resolutions of 2.33 × 10−06 RIU, 4.52 × 10−06 RIU, and 6.17 × 10−06 RIU for ring, trophozoite, and schizont phases, respectively. The obtained amplitude sensitivities are 784.55 RIU−1, 491.02 RIU−1, and 407.99 RIU−1 and FOMs are 596.90 RIU−1, 423.98 RIU−1, and 341.63 RIU−1 for the three phases, respectively. Therefore, with the promising results and simplified practical realization, the proposed sensor can be an excellent candidate for the identification of Plasmodium falciparum phases in RBC for malaria diagnosis.
Exceptional refrigeration of collective motions beyond their mass and temperature limitations
Authors: Liao Deng-Gao , Miranowicz Adam , Nori Franco
Published: May 2023

Coaxing collective vibrations in the regimes of both large mass and high temperature into their motional quantum ground states is extremely challenging, because it requires an ultra-high optical power, which introduces extraneous excessive heating and intricate instabilities. Here we propose how to overcome these obstacles and cool vibrational networks by simply employing an exceptional point (EP) induced in parity-time symmetric structures; and we reveal its exceptional cooling properties otherwise unachievable in conventional devices. In stark contrast to standard-cooling protocols, a three orders-of-magnitude amplification in net-cooling rates arises from the EP-cooling mechanism, without which it vanishes. Remarkably, our EP cooling is nearly immune to both resonator mass and environmental temperature, and this overthrows the consensus that poor intrinsic factors and rugged extrinsic environment suppress cooling channels. Unlike standard-cooling schemes where the aim is to cool and isolate a single vibration, the aim here is to extract phonons from a large number of motions within a large frequency bandwidth. Our study offers the possibility of isolating and engineering collective motional properties of large-mass and high-temperature objects for various applications in acoustic sensing, gravimetry, and inertial navigation.
Integration of a multi-omics stem cell differentiation dataset using a dynamical model
Authors: van den Berg Patrick R. , Bérenger-Currias Noémie M. L. P. , Budnik Bogdan , Slavov Nikolai , Semrau Stefan
Published: May 2023

Stem cell differentiation is a highly dynamic process involving pervasive changes in gene expression. The large majority of existing studies has characterized differentiation at the level of individual molecular profiles, such as the transcriptome or the proteome. To obtain a more comprehensive view, we measured protein, mRNA and microRNA abundance during retinoic acid-driven differentiation of mouse embryonic stem cells. We found that mRNA and protein abundance are typically only weakly correlated across time. To understand this finding, we developed a hierarchical dynamical model that allowed us to integrate all data sets. This model was able to explain mRNA-protein discordance for most genes and identified instances of potential microRNA-mediated regulation. Overexpression or depletion of microRNAs identified by the model, followed by RNA sequencing and protein quantification, were used to follow up on the predictions of the model. Overall, our study shows how multi-omics integration by a dynamical model could be used to nominate candidate regulators.
Green supply chain decision of discarded drugs recycling: Evolutionary game and strategy simulation
Authors: Wang Chao , Huang Zhe , Lian Guiyu
Published: May 2023

The research on the recycling of discarded drugs has become an important direction of the green supply chain in China. Faced with the great challenge of recycling discarded drugs in China, it is necessary to make clear the behavior choice of each recycling subject. This paper constructs an evolutionary game model among government, drugs recycling companies and consumers, describes the evolutionary game process, and analyzes the influence of the gain of government, drug recycling enterprises and consumers on the evolutionary stable strategy trend. By using Matlab simulation software to simulate the evolution of tripartite behavior, the final result is obtained: The government’s choice of a strong regulatory strategy has an important positive role in guiding the entire discarded drug recovery market. Consumer’s choice of active participation strategy and enterprise’s choice of active participation strategy are most beneficial to the healthy development of the discarded drug recovery market. This study provides some valuable theoretical support and reference for the national decision-making of discarded drug recovery, helps to solve the problem of discarded drug recovery, and provides theoretical support and policy-making recommendations for the ultimate achievement of sustainable development goals.
Study protocol for the epigenetic characterization of angor pectoris according to the affected coronary compartment: Global and comprehensive assessment of the relationship between invasive coronary physiology and microRNAs
Authors: Matute-Blanco Lucía , Fernández-Rodríguez Diego , Casanova-Sandoval Juan , Belmonte Thalía , Benítez Iván D. , Rivera Kristian , Garcia-Guimaraes Marcos , Cortés Villar Carlos , Peral Disdier Vicente , Millán Segovia Raúl , Barriuso Ignacio , de Gonzalo-Calvo David , Barbé Ferran , Worner Fernando
Published: May 2023

Background

MicroRNAs (miRNAs) are noncoding RNAs involved in post-transcriptional genetic regulation with a proposed role in intercellular communication. miRNAs are considered promising biomarkers in ischemic heart disease. Invasive physiological evaluation allows a precise assessment of each affected coronary compartment. Although some studies have associated the expression of circulating miRNAs with invasive physiological indexes, their global relationship with coronary compartments has not been assessed. Here, we will evaluate circulating miRNAs profiles according to the coronary pattern of the vascular compartment affectation.

Study and design

This is an investigator-initiated, multicentre, descriptive study to be conducted at three centres in Spain (NCT05374694). The study will include one hundred consecutive patients older than 18 years with chest pain of presumed coronary cause undergoing invasive physiological evaluation, including fractional flow reserve (FFR) and index of microvascular resistance (IMR). Patients will be initially classified into four groups, according to FFR and IMR: macrovascular and microvascular affectation (FFR≤0.80 / IMR≥25), isolated macrovascular affectation (FFR≤0.80 / IMR<25), isolated microvascular affectation (FFR>0.80 / IMR ≥25) and normal coronary indexes (FFR>0.80 / IMR<25). Patients with isolated microvascular affectation or normal indexes will also undergo the acetylcholine test and may be reclassified as a fifth group in the presence of spasm. A panel of miRNAs previously associated with molecular mechanisms linked to chronic coronary syndrome will be analysed using RT-qPCR.

Conclusions

The results of this study will identify miRNA profiles associated with patterns of coronary affectation and will contribute to a better understanding of the mechanistic pathways of coronary pathology.
Predicting resilience during the COVID-19 Pandemic in the United Kingdom: Cross-sectional and longitudinal results
Authors: Bennett Kate M. , Panzeri Anna , Derrer-Merk Elfriede , Butter Sarah , Hartman Todd K. , Mason Liam , McBride Orla , Murphy Jamie , Shevlin Mark , Gibson-Miller Jilly , Levita Liat , Martinez Anton P. , McKay Ryan , Lloyd Alex , Stocks Thomas V. A. , Bottesi Gioa , Vidotto Giulo , Bentall Richard P. , Bertamini Marco
Published: May 2023

Although the COVID-19 pandemic has impacted the psychological wellbeing of some people, there is evidence that many have been much less affected. The Ecological Model of Resilience (EMR) may explain why some individuals are not resilient whilst others are. In this study we test the EMR in a comparison of UK survey data collected from the COVID-19 Psychological Research Consortium (C19PRC) longitudinal study of a representative sample of the United Kingdom (UK) adult population and data from an Italian arm of the study. We first compare data from the third wave of the UK arm of the study, collected in July/August 2020, with data from an equivalent sample and stage of the pandemic in Italy in July 2020. Next, using UK longitudinal data collected from C19PRC Waves 1, 3 and 5, collected between March 2020 and April 2021 we identify the proportion of people who were resilient. Finally, we examine which factors, drawn from the EMR, predict resilient and non-resilient outcomes. We find that the 72% of the UK sample was resilient, in line with the Italian study. In the cross-sectional logistic regression model, age and self-esteem were significantly associated with resilience whilst death anxiety thoughts, neuroticism, loneliness, and Post Traumatic Stress Disorder (PTSD) symptoms related to COVID-19 were significantly associated with Non-Resilient outcomes. In the longitudinal UK analysis, at Wave 5, 80% of the sample was Resilient. Service use, belonging to wider neighbourhood, self-rated health, self-esteem, openness, and externally generated death anxiety were associated with Resilient outcomes. In contrast, PTSD symptoms and loneliness were associated with Non-Resilient outcomes. The EMR effectively explained the results. There were some variables which are amenable to intervention which could increase resilience in the face of similar future challenges.
Waning vaccine response to severe COVID-19 outcomes during omicron predominance in Thailand
Authors: Intawong Kannikar , Chariyalertsak Suwat , Chalom Kittipan , Wonghirundecha Thanachol , Kowatcharakul Woravut , Thongprachum Aksara , Chotirosniramit Narain , Noppakun Kajohnsak , Khwanngern Krit , Teacharak Worachet , Piamanant Prapon , Chantaklang Pannawich , Khammawan Pimpinan
Published: May 2023

Background

The COVID-19 pandemic has evolved quickly, with different variants of concern resulting in the need to offer continued protection through booster vaccinations. The duration of enhanced protection with booster doses against severe COVID-19 is still unclear. Understanding this is critical to recommendations on the frequency of future booster doses.

Methods

Utilising a Hospital Information System for COVID-19 established in Chiang Mai, Thailand, we conducted a cohort study by linking patient-level data of laboratory-confirmed COVID-19 cases to the national immunization records, during the omicron predominant period (1 February– 31 July 2022).

Results

Out of 261,103 adults with COVID-19 included in the study, there were 333 (0.13%) severe COVID-19 cases and 190 (0.07%) deaths. Protection against severe COVID-19 was highest with boosters received >14–60 days prior to positive test (93%) and persisted at >60–120 days (91%) but started to wane at >120–180 days (77%) and further at >180 days (68%). The rate of waning differed with age. Those ≥70 years showed faster waning of booster vaccine responses as compared to those aged 18–49 years, who retained good responses up to 180 days. Equivalent risk reduction against severe COVID-19 was seen with all the vaccine types used as boosters in Thailand.

Conclusions

Booster doses provided high levels of protection against severe COVID-19 with omicron, up to 4 months. Repeat boosters will be required to continue protection beyond 4 months, particularly in the elderly. mRNA and viral vector vaccines can be used flexibly to improve booster coverage.
Exploring the interior of 3D endoluminal lesions in the air spaces by a novel electronic biopsy technique: A preliminary study of endoluminal colon lesions
Authors: Chen Lih-Shyang , Chen Shao-Jer , Hsu Ta-Wen , Chang Shu-Han , Hou Chun-Ju , Lin Chih-Wen , Chen Yu-Ruei , Hsieh Chin-Chiang , Han Shu-Chen , Chang Ku-Yaw
Published: May 2023

To explore the interior of a lesion in a 3D endoluminal view, this study investigates the application of an ‘electronic biopsy’ (EB) technique to computed tomographic colonography (CTC) for further differentiation and 2D image correlation of endoluminal lesions in the air spaces. A retrospective study of sixty-two various endoluminal lesions from thirty patients (13 males, 17 females; age range, 31 to 90 years) was approved by our institutional review board and evaluated. The endoluminal lesions were segmented using gray-level threshold and reconstructed into isosurfaces using a marching cube algorithm. EB allows users to interactively erode and apply grey-level mapping (GM) to the surface of the region of interest (ROI) in 3D CTC. Radiologists conducted the clinical evaluation, and the resulting data were analyzed. EB significantly improves 3D gray-level presentation for evaluating the surface and inside of endoluminal lesions over that of SR, GM or target GM (TGM) (P < 0.01) with preservation of the 3D spatial effect. Moreover, 3D to 2D image correlation were achieved in any layer of the lesion using EB as did GM/TGM on the surface. The specificity and diagnostic accuracy of EB are significantly greater than those of SR (P < 0.01). These performance can be better further with GM/TGM and reach the best with EB (specificity, 89.3–92.9%; accuracy, 95.2–96.8%). EB can be used in CTC to improve the differentiation of endoluminal lesions. EB increases 3D to 2D image correlations of the lesions on or beneath the lesion surface.
Unsaturated or saturated dietary fat-mediated steatosis impairs hepatic regeneration following partial hepatectomy in mice
Authors: Islam S. M. Touhidul , Palanisamy Arun P. , Chedister Gabriel R. , Schmidt Michael G. , Lewin David N. B. , Chavin Kenneth D.
Published: May 2023

Background

Partial hepatectomy is a preferred treatment option for many patients with hepatocellular carcinoma however, pre-existing pathological abnormalities originating from hepatic steatosis can alter the decision to perform surgery or postoperative outcomes as a consequence of the impact steatosis has on liver regeneration.

Aim

The aim of this study was to investigate the role of a saturated or unsaturated high fat diet-mediated steatosis on liver regeneration following partial hepatectomy.

Methods

Mice were fed a low-fat control diet (CD, 13% fat), lard-based unsaturated (LD, 60% fat) or milk-based saturated high fat diet (MD, 60% fat) for 16 weeks at which time partial hepatectomy (approx. 70% resection) was performed. At days-2 and 7 post hepatectomy, one hour prior to euthanization, mice were injected with 5-bromo-2’-deoxyuridine in order to monitor hepatic regeneration. Serum was collected and assessed for levels of ALT and AST. Resected and regenerated liver tissue were examined for inflammation-indicative markers employing RT-PCR, Western blots, and histological methods.

Results

Mice fed LD or MD exhibited higher NAFLD scores, increased expression of inflammatory cytokines, neutrophil infiltration, macrophage accumulation, increased apoptosis, and elevated levels of serum ALT and AST activities, a decrease in the number of BrdU-incorporated-hepatocytes in the regenerated livers compared to the mice fed CD. Mice fed MD showed significantly lower percent of BrdU-incorporated hepatocytes and a higher trend of inflammation compared to the mice fed LD.

Conclusion

A diet rich in saturated or unsaturated fat results in NASH with decreased hepatic regeneration however unsaturated fat diet cause lower inflammation and higher regeneration than the saturated fat diet following partial hepatectomy in mice.
Social media engagement and cryptocurrency performance
Authors: Qureshi Khizar , Zaman Tauhid
Published: May 2023

Cryptocurrencies are highly speculative assets with large price volatility. If one could forecast their behavior, this would make them more attractive to investors. In this work we study the problem of predicting the future performance of cryptocurrencies using social media data. We propose a new model to measure the engagement of users with topics discussed on social media based on interactions with social media posts. This model overcomes the limitations of previous volume and sentiment based approaches. We use this model to estimate engagement coefficients for 48 cryptocurrencies created between 2019 and 2021 using data from Twitter from the first month of the cryptocurrencies’ existence. We find that the future returns of the cryptocurrencies are dependent on the engagement coefficients. Cryptocurrencies whose engagement coefficients have extreme values have lower returns. Low engagement coefficients signal a lack of interest, while high engagement coefficients signal artificial activity which is likely from automated accounts known as bots. We measure the amount of bot posts for the cryptocurrencies and find that generally, cryptocurrencies with more bot posts have lower future returns. While future returns are dependent on both the bot activity and engagement coefficient, the dependence is strongest for the engagement coefficient, especially for short-term returns. We show that simple investment strategies which select cryptocurrencies with engagement coefficients exceeding a fixed threshold perform well for holding times of a few months.
Resting heart rate is a population-level biomarker of cardiorespiratory fitness: The Fenland Study
Authors: Gonzales Tomas I. , Jeon Justin Y. , Lindsay Timothy , Westgate Kate , Perez-Pozuelo Ignacio , Hollidge Stefanie , Wijndaele Katrien , Rennie Kirsten , Forouhi Nita , Griffin Simon , Wareham Nick , Brage Soren
Published: May 2023

Introduction

Few large studies have evaluated the relationship between resting heart rate (RHR) and cardiorespiratory fitness. Here we examine cross-sectional and longitudinal relationships between RHR and fitness, explore factors that influence these relationships, and demonstrate the utility of RHR for remote population monitoring.

Methods

In cross-sectional analyses (The UK Fenland Study: 5,722 women, 5,143 men, aged 29-65y), we measured RHR (beats per min, bpm) while seated, supine, and during sleep. Fitness was estimated as maximal oxygen consumption (ml⋅min-1⋅kg-1) from an exercise test. Associations between RHR and fitness were evaluated while adjusting for age, sex, adiposity, and physical activity. In longitudinal analyses (6,589 participant subsample), we re-assessed RHR and fitness after a median of 6 years and evaluated the association between within-person change in RHR and fitness. During the coronavirus disease-2019 pandemic, we used a smartphone application to remotely and serially measure RHR (1,914 participant subsample, August 2020 to April 2021) and examined differences in RHR dynamics by pre-pandemic fitness level.

Results

Mean RHR while seated, supine, and during sleep was 67, 64, and 57 bpm. Age-adjusted associations (beta coefficients) between RHR and fitness were -0.26, -0.29, and -0.21 ml⋅kg-1⋅beat-1 in women and -0.27, -0.31, and -0.19 ml⋅kg-1⋅beat-1 in men. Adjustment for adiposity and physical activity attenuated the RHR-to-fitness relationship by 10% and 50%, respectively. Longitudinally, a 1-bpm increase in supine RHR was associated with a 0.23 ml⋅min-1⋅kg-1 decrease in fitness. During the pandemic, RHR increased in those with low pre-pandemic fitness but was stable in others.

Conclusions

RHR is a valid population-level biomarker of cardiorespiratory fitness. Physical activity and adiposity attenuate the relationship between RHR and fitness.
Eliminating the need for manual segmentation to determine size and volume from MRI. A proof of concept on segmenting the lateral ventricles
Authors: Yepes-Calderon Fernando , McComb J. Gordon
Published: May 2023

Manual segmentation, which is tedious, time-consuming, and operator-dependent, is currently used as the gold standard to validate automatic and semiautomatic methods that quantify geometries from 2D and 3D MR images. This study examines the accuracy of manual segmentation and generalizes a strategy to eliminate its use. Trained individuals manually measured MR lateral ventricles images of normal and hydrocephalus infants from 1 month to 9.5 years of age. We created 3D-printed models of the lateral ventricles from the MRI studies and accurately estimated their volume by water displacement. MRI phantoms were made from the 3D models and images obtained. Using a previously developed artificial intelligence (AI) algorithm that employs four features extracted from the images, we estimated the ventricular volume of the phantom images. The algorithm was certified when discrepancies between the volumes—gold standards—yielded by the water displacement device and those measured by the automation were smaller than 2%. Then, we compared volumes after manual segmentation with those obtained with the certified automation. As determined by manual segmentation, lateral ventricular volume yielded an inter and intra-operator variation up to 50% and 48%, respectively, while manually segmenting saggital images generated errors up to 71%. These errors were determined by direct comparisons with the volumes yielded by the certified automation. The errors induced by manual segmentation are large enough to adversely affect decisions that may lead to less-than-optimal treatment; therefore, we suggest avoiding manual segmentation whenever possible.
3D doppler ultrasound imaging of cerebral blood flow for assessment of neonatal hypoxic-ischemic brain injury in mice
Authors: Shen Guofang , Sanchez Kayla , Hu Shirley , Zhao Zhen , Zhang Lubo , Ma Qingyi
Published: May 2023

Cerebral blood flow (CBF) acutely reduces in neonatal hypoxic-ischemic encephalopathy (HIE). Clinic studies have reported that severe CBF impairment can predict HIE outcomes in neonates. Herein, the present study uses a non-invasive 3D ultrasound imaging approach to evaluate the changes of CBF after HI insult, and explores the correlation between CBF alterations and HI-induced brain infarct in mouse pups. The neonatal HI brain injury was induced in postnatal day 7 mouse pups using the Rice-Vannucci model. Non-invasive 3D ultrasound imaging was conducted to image CBF changes with multiple frequencies on mouse pups before common carotid artery (CCA) ligation, immediately after ligation, and 0 or 24 hours after HI. Vascularity ratio of the ipsilateral hemisphere was acutely reduced after unilateral ligation of the CCA alone or in combination with hypoxia, and partially restored at 24 hours after HI. Moreover, regression analysis showed that the vascularity ratio of ipsilateral hemisphere was moderately correlated with brain infarct size 24 hours after HI, indicating that CBF reduction contributes to of HI brain injury. To further verify the association between CBF and HI-induced brain injury, a neuropeptide C-type natriuretic peptide (CNP) or PBS was intranasally administrated to the brain of mouse pups one hour after HI insult. Brain infarction, CBF imaging and long-term neurobehavioral tests were conducted. The result showed that intranasal administration of CNP preserved ipsilateral CBF, reduced the infarct size, and improved neurological function after HI brain injury. Our findings suggest that CBF alteration is an indicator for neonatal HI brain injury, and 3D ultrasound imaging is a useful non-invasive approach for assessment of HI brain injury in mouse model.
Congenital ventricular outflow tract obstructions in Boxer dogs: Results of a 17-year cardiovascular breed screening program in France (3126 dogs)
Authors: Chetboul Valérie , Bernard Pauline , Passavin Peggy , Tissier Renaud
Published: May 2023

Introduction

Ventricular outflow tract obstructions including aortic (AS) and pulmonic stenosis (PS) are the most common canine congenital heart diseases, with Boxer dogs being predominantly affected. This has led to the French Boxer club adopting a mandatory national control program against AS and PS. The objective of this retrospective study was to analyze the results of 17 years of this cardiovascular breed screening program (2005–2021).

Materials and methods

The records of untreated and non-anesthetized adult Boxer dogs screened between 2005 and 2021 were retrospectively reviewed. All dogs underwent physical examination and standard transthoracic echocardiography with concomitant ECG tracing. All examinations were reviewed by one single board-certified specialist in cardiology.

Results

Out of the 3126 dogs screened during the study period, 3001 dogs (female:male sex ratio = 2.2, median age [IQR] = 1.6 years [1.2–2.1]) were recruited for data analysis. A total of 218 operators were involved in the screening program. For most Boxer dogs (i.e., 93.8% for AS and 94.5% for PS), a single examination was required to obtain a definitive cardiac status, although most operators were non-specialist general practitioners. A left basilar systolic heart murmur was detected in all dogs with AS and PS, but also in 7.4% dogs free of heart diseases. A significantly higher proportion of the latter was detected when operators were board-certified specialists (P<0.001). Lastly, when comparing the start and the end of the breeding program, among dogs diagnosed with AS and PS (n = 364) in a French referral cardiology center, Boxer went from the 1st affected breed by AS to the 3rd, and from the 3rd affected breed by PS to the 6th.

Conclusion

This 17-year screening program has experienced a strong involvement of veterinarians, breeders, and owners throughout France. This may have contributed to reduce AS and PS prevalence in Boxer dogs at the studied referral cardiology center.
Structural requirements of Holothuria floridana fucosylated chondroitin sulfate oligosaccharides in anti-SARS-CoV-2 and anticoagulant activities
Authors: Farrag Marwa , Dwivedi Rohini , Sharma Poonam , Kumar Deepak , Tandon Ritesh , Pomin Vitor H.
Published: May 2023

Fucosylated chondroitin sulfate (FucCS) is a unique glycosaminoglycan found primarily in sea cucumbers. This marine sulfated glycan is composed of a chondroitin sulfate backbone decorated with fucosyl branches attached to the glucuronic acid. FucCS exhibits potential biological actions including inhibition of blood clotting and severe acute respiratory syndrome coronavirus (SARS-CoV-2) infection. These biological effects have been attributed to certain structural features, including molecular weight (MW), and/or those related to fucosylation, such as degrees of fucosyl branches, sulfation patterns and contents. In a previous work, we were able to generate oligosaccharides of the FucCS from Pentacta pygmaea (PpFucCS) with reduced anticoagulant effect but still retaining significant anti-SARS-CoV-2 activity against the delta strain. In this work, we extended our study to the FucCS extracted from the species Holothuria floridana (HfFucCS). The oligosaccharides were prepared by free-radical depolymerization of the HfFucCS via copper-based Fenton reaction. One-dimensional 1H nuclear magnetic resonance spectra were employed in structural analysis. Activated partial thromboplastin time and assays using protease (factors Xa and IIa) and serine protease inhibitors (antithrombin, and heparin cofactor II) in the presence of the sulfated carbohydrates were used to monitor anticoagulation. Anti-SARS-CoV-2 effects were measured using the concentration–response inhibitory curves of HEK-293T-human angiotensin-converting enzyme-2 cells infected with a baculovirus pseudotyped SARS-CoV-2 wild-type and delta variant spike (S)-proteins. Furthermore, the cytotoxicity of native HfFucCS and its oligosaccharides was also assessed. Like for PpFucCS, we were able to generate a HfFucCS oligosaccharide fraction devoid of high anticoagulant effect but still retaining considerable anti-SARS-CoV-2 actions against both variants. However, compared to the oligosaccharide fraction derived from PpFucCS, the average MW of the shortest active HfFucCS oligosaccharide fraction was significantly lower. This finding suggests that the specific structural feature in HfFucCS, the branching 3,4-di-sulfated fucoses together with the backbone 4,6-di-sulfated N-acetylgalactosamines, is relevant for the anti-SARS-CoV-2 activity of FucCS molecules.
Bidirectional relationship between sexual arousal and (sex-related) disgust
Authors: Wen Guangju , Niu Caoyuan , Zhang Yikang , Santtila Pekka
Published: May 2023

Sexual stimuli provoke both sexual arousal and disgust, and the coaction between these emotions determines sexually behavioral outcomes. The current research includes two experiments to explore the bidirectional relationship between sexual arousal toward erotic stimuli and disgust induced by sexual body fluids. Study 1 presented 234 participants (117 women) with sexual body (vs. neutral) fluids followed by erotic stimuli, and Study 2 presented 235 participants (117 women) with erotic (vs. neutral) videos followed by sexual body fluids (and a non-sex-related stimulus). Study 1 showed that exposure to sexual body fluids reduced sexual arousal and the likelihood of sexual engagement toward erotic stimuli in participants with high sexual disgust sensitivity but increased sexual arousal and the likelihood of sexual engagement in participants with low sexual disgust sensitivity, while Study 2 suggested that men exposed to erotic (vs. neutral) stimuli reported lower disgust, stronger sexual arousal state, and higher willingness to interact with the sexual body fluids. There was no relationship between subjective feelings of sexual arousal and disgust in these experiments, while the balance of sexual arousal and disgust toward sexual body fluids and erotic stimuli had a positive association. Also, exposure to erotic stimuli had no effect on reactions to generally disgusting stimulus, but feelings of sexual arousal toward erotic stimuli were positively associated with disgust induced by generally disgusting fluid. These findings suggest that Behavior Immune System regulates disgust to establish a balance between benefit and cost related to sex as well as provide insight into the process underlying sexual dysfunctions.
Evaluation of the accuracy of the IDvet serological test for Mycoplasma bovis infection in cattle using latent class analysis of paired serum ELISA and quantitative real-time PCR on tonsillar swabs sampled at slaughter
Authors: Marquetoux Nelly , Vignes Matthieu , Burroughs Amy , Sumner Emma , Sawford Kate , Jones Geoff
Published: May 2023

Mycoplasma bovis (Mbovis) was first detected in cattle in New Zealand (NZ) in July 2017. To prevent further spread, NZ launched a world-first National Eradication Programme in May 2018. Existing diagnostic tests for Mbovis have been applied in countries where Mbovis is endemic, for detecting infection following outbreaks of clinical disease. Diagnostic test evaluation (DTE) under NZ conditions was thus required to inform the Programme. We used Bayesian Latent Class Analysis on paired serum ELISA (ID Screen Mycoplasma bovis Indirect from IDvet) and tonsillar swabs (qPCR) for DTE in the absence of a gold standard. Tested samples were collected at slaughter between June 2018 and November 2019, from infected herds depopulated by the Programme. A first set of models evaluated the detection of active infection, i.e. the presence of Mbovis in the host. At a modified serology positivity threshold of SP%> = 90, estimates of animal-level ELISA sensitivity was 72.8% (95% credible interval 68.5%—77.4%), respectively 97.7% (95% credible interval 97.3%—98.1%) for specificity, while the qPCR sensitivity was 45.2% (95% credible interval 41.0%—49.8%), respectively 99.6% (95% credible interval 99.4%—99.8%) for specificity. In a second set of models, prior information about ELISA specificity was obtained from the National Beef Cattle Surveillance Programme, a population theoretically free—or very low prevalence—of Mbovis. These analyses aimed to evaluate the accuracy of the ELISA test targeting prior exposure to Mbovis, rather than active infection. The specificity of the ELISA for detecting exposure to Mbovis was 99.9% (95% credible interval 99.7%—100.0%), hence near perfect at the threshold SP%=90. This specificity estimate, considerably higher than in the first set of models, was equivalent to the manufacturer’s estimate. The corresponding ELISA sensitivity estimate was 66.0% (95% credible interval 62.7%-70.7%). These results confirm that the IDvet ELISA test is an appropriate tool for determining exposure and infection status of herds, both to delimit and confirm the absence of Mbovis.
PM2.5 concentration assessment based on geographical and temporal weighted regression model and MCD19A2 from 2015 to 2020 in Xinjiang, China
Authors: Quan Weilin , Xia Nan , Guo Yitu , Hai Wenyue , Song Jimi , Zhang Bowen
Published: May 2023

PM2.5 is closely linked to both air quality and public health. Many studies have used models combined with remote sensing and auxiliary data to inverse a large range of PM2.5 concentrations. However, the data’s spatial resolution is limited. and better results might have been obtained if higher resolution data had been used. Therefore, this paper establishes a geographical and temporal weighted regression model (GTWR) and estimates the PM2.5 concentration in Xinjiang from 2015 to 2020 using 1 km resolution MCD19A2 (MODIS/Terra+Aqua Land Aerosol Optical Thickness Daily L2G Global 1km SIN Grid V006) data and 9 auxiliary variables. The findings indicate that the GTWR model performs better than the simple linear regression (SLR) and geographically weighted regression (GWR) models in terms of accuracy and feasibility in retrieving PM2.5 concentrations in Xinjiang. Simultaneously, by combining the GTWR model with MCD19A2 data, a spatial distribution map of PM2.5 with better spatial resolution can be obtained. Next, the regional distribution of annual PM2.5 concentrations in Xinjiang is consistent with the terrain from 2015 to 2020. The low value area is primarily found in the mountainous area with higher terrain, while the high value area is primarily in the basin with lower terrain. Overall, the southwest is high and the northeast is low. In terms of time change, the six-year PM2.5 shows a single peak distribution with 2016 as the inflection point. Lastly, from 2015 to 2020, the seasonal average PM2.5 concentration in Xinjiang has a significant difference, thereby showing winter (66.15μg/m3)>spring (52.28μg/m3)>autumn (40.51μg/m3)>summer (38.63μg/m3). The research shows that the combination of MCD19A2 data and GTWR model has good applicability in retrieving PM2.5 concentration.
Evaluation of microbial and vancomycin treatments in ulcerative colitis in murine models
Authors: Hasan Nihal , Yang Hongyi
Published: May 2023

Background

Despite the number of available therapies for ulcerative colitis (UC), severe side effects and high cost has limited their clinical application. Thus, finding new alternative strategies with minimal side effects is inevitable. Therefore, this study aimed to compare the effectiveness of different therapeutic approaches in DSS-induced colitis.

Methods

Firstly, we designed oral bio-therapeutic products, Live Bacterial Products (LBP), which include a mixture of fecal bacteria strains isolated from healthy mice and prepared by microencapsulation and freeze-dried techniques. Then we investigated the efficiency of 7 days of freeze-dried FMT, LBP, and vancomycin treatments in DSS-induced colitis. Secondly, we compared the effect of 15 days of microbial therapies (freeze-dried powder of FMT and LBP microcapsules) and seven days of oral vancomycin on the severity of colitis in mice. Furthermore, the levels of IL-1β and TNF-α were measured in serum by ELISA, and the fecal microbiota diversity was analyzed by high-throughput sequencing for all mice groups.

Results

After seven days of treatments, our results indicated that oral vancomycin reduced the severity of DSS-induced colitis in mice, where weight gain and a decrease in IL-1 β and TNF-α levels were observed in the vancomycin group compared with other treatment groups. While after two weeks of treatment, the LBP microcapsules were able to reduce the severity of colitis. And at the end of the treatment period, weight gain and a decrease in the DAI scores and the levels of IL-1β and TNF-α were noted in the LBP treatment group compared to other treatment groups. By high-throughput sequencing of the 16S rRNA gene, our results showed that while the microcapsules LBP treatment increased the fecal microbial diversity, after vancomycin therapy, most of the fecal microbiota genera and operational taxonomic units (OTUs) were depleted.

Conclusion

Our results concluded that treatment duration and preparation methods affect the microbial therapies’ efficiency in UC. Furthermore, this study highlighted the negative consequences of oral vancomycin administration on gut health that should be known before using this medication.
Psychological burden of achalasia: Patients’ screening rates of depression and anxiety and sex differences
Authors: Hanschmidt Franz , Treml Julia , Deller Julia , Kreuser Nicole , Gockel Ines , Kersting Anette
Published: May 2023

Objective

Achalasia is associated with reduced quality of life in affected patients but research regarding the psychological burden of achalasia in terms of depression and anxiety is scarce. The current study therefore aims to investigate rates of depression and anxiety in patients with achalasia in relation to prevalence rates in the general population and to examine the extent to which achalasia-related characteristics (time since diagnosis, symptom load, achalasia-related quality of life, treatment history) predict symptoms of depression and anxiety.

Methods

Using validated screening instruments, rates of depression and anxiety were assessed in a cross-sectional survey of a sample of 993 patients with achalasia and compared to population controls stratified by age and sex. Associations between depression and anxiety and achalasia-related factors were explored using linear regression.

Results

Compared to population controls, screening rates of female patients with achalasia were between 3.04 (p = .004) and 7.87 (p < .001) times higher for depression and 3.10 (p < .001) times higher for anxiety, respectively. No significant differences were found for male patients with achalasia. Both achalasia-related quality of life and symptom load were independently related to impaired mental health.

Conclusion

Women appear to be specifically affected by the psychological burden of achalasia, pointing to sex-specific or gendered experiences of the disease. In addition to symptom reduction, psychological support may prove beneficial for improving the well-being of patients with achalasia.