bluuebunny/crossref_metadata_2025 huggingface dataset:
Fitness Facility Staff Can Be Trained to Deliver a Motivational Interviewing-Informed Diabetes Prevention Program
Authors: Dineen Tineke E. , Bean Corliss , Cranston Kaela D. , MacPherson Megan M. , Jung Mary E.
Published: December 2021

Background: Training programs must be evaluated to understand whether the training was successful at enabling staff to implement a program with fidelity. This is especially important when the training has been translated to a new context. The aim of this community case study was to evaluate the effectiveness of the in-person Small Steps for Big Changes training for fitness facility staff using the 4-level Kirkpatrick training evaluation model.

Methods: Eight staff were trained to deliver the motivational interviewing-informed Small Steps for Big Changes program for individuals at risk of developing type 2 diabetes. Between August 2019 and March 2020, 32 clients enrolled in the program and were allocated to one of the eight staff. The Kirkpatrick 4-level training evaluation model was used to guide this research. Level one assessed staff satisfaction to the training on a 5-point scale. Level two assessed staff program knowledge and motivational interviewing knowledge/skills. Level three assessed staff behaviors by examining their use of motivational interviewing with each client. Level four assessed training outcomes using clients' perceived satisfaction with their staff and basic psychological needs support both on 7-point scales.

Results: Staff were satisfied with the training (M = 4.43; SD = 0.45; range = 3.86–4.71). All learning measures demonstrated high post-training scores that were retained at implementation follow-up. Staff used motivational interviewing skills in practice and delivered the program at a client-centered level (≥6; M = 6.34; SD = 0.83; range = 3.75–7.80). Overall, clients perceived staff supported their basic psychological needs (M = 6.55; SD = 0.64; range = 6.17–6.72) and reported high staff satisfaction scores (M = 6.88; SD = 0.33; range = 6–7).

Conclusion: The Small Steps for Big Changes training was successful and fitness facility staff delivered a motivational interviewing-informed program. While not all staff operated at a client-centered level, clients perceived their basic psychological needs to be supported. Findings support the training for future scale-up sites. Community fitness staff represent a feasible resource through which to run evidence-based counseling programs.
Lipidomic Analyses Uncover Apoptotic and Inhibitory Effects of Pyrvinium Pamoate on Cholangiocarcinoma Cells via Mitochondrial Membrane Potential Dysfunction
Authors: Kittirat Yingpinyapat , Phetcharaburanin Jutarop , Promraksa Bundit , Kulthawatsiri Thanaporn , Wangwiwatsin Arporn , Klanrit Poramate , Sangkhamanon Sakkarn , Jarearnrat Apiwat , Thongchot Suyanee , Mahalapbutr Panupong , Loilome Watcharin , Saya Hideyuki , Namwat Nisana
Published: December 2021

Pyrvinium pamoate (PP), an FDA-approved anthelmintic drug, has been validated as a highly potent anti-cancer agent and patented recently as a potential chemotherapeutic drug for various cancers. The aims of this study were, therefore, to investigate the ability of PP in anti-proliferative activity and focused on the lipid profiles revealing the alteration of specific lipid species in the liver fluke Opisthorchis viverrini (Ov)-associated cholangiocarcinoma (CCA) cells. PP inhibited CCA cell viability through suppressing mitochondrial membrane potential (MMP) and ATP productions, leading to apoptotic cell death. Liquid chromatography-mass spectrometry combined with chemometrics was performed to investigate lipid alteration during PP-induced apoptosis. The lipidomic analyses showed the altered lipid signatures of CCA cell types including S-acetyldihydrolipoamide, methylselenopyruvate, and triglycerides that were increased in PP-treated CCA cells. In contrast, the levels of sphinganine and phosphatidylinositol were lower in the PP-treated group compared with its counterpart. The orthogonal partial-least squares regression analysis revealed that PP-induced MMP dysfunction, leading to remarkably reduced ATP level, was significantly associated with triglyceride (TG) accumulation observed in PP-treated CCA cells. Our findings indicate that PP could suppress the MMP function, which causes inhibition of CCA cell viability through lipid production, resulting in apoptotic induction in CCA cells. These findings provide an anti-cancer mechanism of PP under apoptotic induction ability that may serve as the alternative approach for CCA treatment.
Microablative Fractional Radiofrequency as a Therapeutical Option for Genitourinary Syndrome of Menopause: Perspectives
Authors: Kamilos Márcia Farina , Costa Ana Paula Ferreira , Sarmento Ayane Cristine Alves , Eleutério José , Gonçalves Ana Katherine
Published: December 2021

The genitourinary syndrome in menopause can occur at different stages of life, with different causes or triggering factors, such as prolonged use of antiestrogens, chemotherapy, radiotherapy, and extensive vaginal surgeries, which can alter vascularization, hydration, collagen quality, and tissue elasticity. Despite hormonal therapy being considered the best evidenced treatment for genitourinary syndrome of menopause (GSM), there are limitations concerning the latter. Thus, alternative, complementary, or even substitutive treatments have emerged, such as energy use, promoting thermal tissue stimulation to improve tropism. Due to its practicality and feasibility, the micro ablative fractional radiofrequency (MAFRF) has gained space among these energies. It uses high-frequency electromagnetic waves and promotes thermal micro points in the superficial and deep dermis. The safety of these energies limits thermal action laterality and depth. Laterally, it is essential for an adequate regenerative effect without scarring marks or sequelae; the appropriate depth is important for stimulating the obligatory tissue repair response with the production and reorganization of collagen, elastic fibers, increased vascularization and hydration, and the consequent improvement in tropism. In gynecology, the MAFRF is used with therapeutic indication and functional improvement; it is applied to the entire length of the vaginal walls, the vulvar vestibule, urethral meatus, labia minora, clitoris prepuce, labia majora, perineum, and perianal region. The MAFRF has been proved to be an effective and safe treatment for GSM, with long-lasting effects, significantly reducing symptoms and improving vaginal tropism. This review aims to analyze the MAFRF as a non-hormonal therapeutic option for GSM.
Share Rose, Get Fun: The Influence of Donation on Happiness
Authors: Wu Yuan-yang , Yu Yi-tong , Yao Yi-dan , Su Mo-han , Zhang Wen-chao , Ti Shi-ming , Lin Xue-yu , Zhang Shuo , Zhang Si-qing , Yang Hua-lei
Published: December 2021

There is little literature on the impact of donation on individual wellbeing in China. This study examines individual donations in China to answer the question of whether helping others makes us happier and to provide policy implications for in Chinese context. Based on the 2012 Chinese General Social Survey (CGSS) data and using ordered logit and OLS as benchmark models, this study finds that donation can significantly increase individual happiness. After using propensity score matching (PSM) to eliminate the possible impact of self-selection, the above conclusion remains robust. After a sub-sample discussion, it is found that this effect is more pronounced under completely voluntary donation behavior, and is not affected by economic factors, indicating that the happiness effect of donation does not vary significantly depending on the individual’s economic status. This study contributes to the literature on donation behavior by examining the impact of donation behavior on donors’ subjective happiness in China, and further identifies subjective happiness differences, as between voluntary and involuntary donations, thereby providing theoretical and empirical support for the formulation of policies for the development of donation institutions in China.
A Retrospective Analysis: Autologous Peripheral Blood Hematopoietic Stem Cell Transplant Combined With Adoptive T-Cell Therapy for the Treatment of High-Grade B-Cell Lymphoma in Ten Dogs
Authors: Gareau Alexandra , Ripoll Alexandra Z. , Suter Steven E.
Published: December 2021

In humans, a type of cellular immunotherapy, called adoptive T cell transfer (ACT), can elicit curative responses against hematological malignancies and melanoma. ACT using ex vivo expanded peripheral blood T-cells after multiagent chemotherapy enhances tumor-free survival of dogs with B-cell lymphoma (LSA). Since 2008, our group has been performing autologous peripheral blood hematopoietic stem cell transplants (autoPBHSCT) for the treatment of canine high-grade B-cell LSA, although relapse of residual disease is a common cause of reduced survival in ~70% of treated dogs. We reasoned that a more aggressive treatment protocol combining CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone) chemotherapy, autoPBHSCT, and ACT to treat 10 dogs with B-cell LSA could lead to better outcomes when compared to dogs treated with CHOP chemotherapy and autoPBHSCT alone. Using this protocol, once dogs achieved complete hematologic reconstitution post-autoPBHSCT, CD3+ CD8+ and CD3+CD4+ T-cells were expanded from the peripheral blood at a commercial laboratory. Two to four ACT infusions were given to each dog, with a total of 23 infusions given. Infusions were administered with no complications or adverse events. The median cell dose for all infusions was 5.62 x 106 cells/kg (range: 2.59 x 106-8.55 x 106 cells/kg). 4/10 (40%) of dogs were cured of their disease (defined as disease-free for ≥2 years post-autoPBHSCT). Our results confirm that the autoPBHSCT protocol did not hinder the in vitro expansion of autologous peripheral blood T-cells and that the final product could be administered safely, with no adverse events recorded. Finally, since only ten dogs were treated, our results can only suggest that the administration of ACT to dogs after multiagent chemotherapy and autoPHSCT did not lead to a statistically significant increase in median disease-free interval and overall survival when compared to dogs who received CHOP chemotherapy and autoPHSCT alone.
Special Issue “Technologies for Future Distributed Engine Control Systems”
Authors: Przysowa Radoslaw
Published: December 2021

Current trends in aviation greatly expand the use of highly integrated, increasingly autonomous air vehicles, with distributed engine control systems (DECS) [...]
Prediction and Validation of Landing Stability of a Lunar Lander by a Classification Map Based on Touchdown Landing Dynamics’ Simulation Considering Soft Ground
Authors: Kim Yeong-Bae , Jeong Hyun-Jae , Park Shin-Mu , Lim Jae Hyuk , Lee Hoon-Hee
Published: December 2021

In this paper, a method for predicting the landing stability of a lunar lander by a classification map of the landing stability is proposed, considering the soft soil characteristics and the slope angle of the lunar surface. First, the landing stability condition in terms of the safe (=stable), sliding (=unstable), and tip-over (=statically unstable) possibilities was checked by dropping a lunar lander onto flat lunar surfaces through finite-element (FE) simulation according to the slope angle, friction coefficient, and soft/rigid ground, while the vertical touchdown velocity was maintained at 3 m/s. All of the simulation results were classified by a classification map with the aid of logistic regression, a machine-learning classification algorithm. Finally, the landing stability status was efficiently predicted by Monte Carlo (MC) simulation by just referring to the classification map for 10,000 input datasets, consisting of the friction coefficient, slope angles, and rigid/soft ground. To demonstrate the performance, two virtual lunar surfaces were employed based on a 3D terrain map of the LRO mission. Then, the landing stability was validated through landing simulation of an FE model of a lunar lander requiring high computation cost. The prediction results showed excellent agreement with those of landing simulations with a negligible computational cost of around a few seconds.
Biological Indicators of Soil Condition on the Kabanyolo Experimental Field, Uganda
Authors: Ivanova Anna , Denisova Elizaveta , Musinguzi Patrick , Opolot Emmanuel , Tumuhairwe John Baptist , Pozdnyakov Lev , Manucharova Natalia , Ilichev Igor , Stepanov Aleksey , Krasilnikov Pavel
Published: December 2021

Soil biological activity is an integral characteristic reflecting the state of soil fertility, biodiversity, and the activity of soil processes carried out by soil organisms. In Africa, studies of soil biological properties are few compared to the agrochemical research. In this paper, we present an assessment of multiple biochemical and microbiological properties of soil from an agricultural field located in the African tropical savanna. We determined basal respiration, substrate-induced respiration, C of microbial biomass, the potential activity of denitrification, nitrogen fixation activity, and estimated prokaryotic components in the soil microbial complex by quantitative PCR. Basal respiration of soils ranged from 0.77 ± 0.04 to 1.90 ± 0.23 μg C-CO2·g−1·h−1, and substrate-induced respiration ranged from 3.31 ± 0.17 to 7.84 ± 1.04 μg C-CO2·g−1·h−1. The C reserves of microbial biomass averaged 403.7 ± 121.6 μg C·g−1 of soil. The N2O emission from the upper layer on average amounted to 2.79 ng N-N2O·g−1·day−1, and the potential denitrification activity reached 745 ± 98 ng N-N2O·g−1·h−1. The number of copies of bacterial genes varied from (0.19 ± 0.02) × 108 to (3.52 ± 0.8) × 108 copies·g−1, and of archaea—from (0.10 ± 0.01) × 107 to (0.29 ± 0.01) × 107 copies·g−1 of soil. These results were in good agreement with the studies in other seasonally wet tropical regions: the biological activity was relatively low. The difference between biological indicators of the experimental field and the reference profile were insignificant except for nitrogen loss, which was higher in the ploughed field. Biological indicators strongly varied in space; we explained their heterogeneity by non-uniform management practices in the course of agrochemical field experiments in the past. The use of organic fertilisers may cause the release of climatically active gases due to intensive microbial respiration and denitrification, but the intensity of emission would strongly depend on the cultivation and management method.
The Influence of Parental Heat-Stress Priming on Drought-Tolerant Maize Progenies’ Field Performance
Authors: Chukwudi Uchechukwu Paschal , Kutu Funso Raphael , Mavengahama Sydney
Published: December 2021

Maize (Zea mays L.) is a staple crop and an industrial crop. Improving its thermotolerance will be a crucial step in ensuring food security. The objective of this research was to assess the influence of the parental growth environment on their progenies in a non-heat stress environment. The progenies evaluated in this field study were obtained from a 2 × 3 × 3 factorial in a completely randomised greenhouse experiment. Two contrasting growth environments, three maize varieties, and three soil amendments were used in the greenhouse study. A randomised complete block design experiment with three replications was used to grow the progenies. The progenies were examined for nineteen morphological attributes. In this study, 69.51% of the yield variation was explained by the first and second principal component axes. Among the studied attributes, grain weight and cob weight explained more variations in the progenies than the other attributes. The interaction of the parental heat-stress and soil amendment conditions elicited different responses from the drought-tolerant maize progenies. Based on the differences in their yield attributes, the progenies were grouped as poor yielders (Cluster IV), good yielders (Cluster I) and high yielders (Clusters II and III). The parental growth environment influenced the progenies’ field performance in a non-heat-stress environment. Further evaluation of the progenies under a heat-stress environment and molecular analyses are required to establish that a transgenerational effect has occurred.
An Assessment of Poverty Alleviation Measures and Sustainable Livelihood Capability of Farm Households in Rural China: A Sustainable Livelihood Approach
Authors: Su Fang , Song Nini , Ma Nannan , Sultanaliev Altynbek , Ma Jing , Xue Bing , Fahad Shah
Published: December 2021

This paper aims to identify effective mechanisms for government poverty alleviation measures based on the livelihood sustainability of farm households in Southern Shaanxi province, China. The paper utilizes data from 414 farm households, collected through field observations and in-depth interviews in 24 rural communes in Qinba Mountain Area of Shaanxi province, China. Using theoretical research methods and employing the sustainable livelihood approach (SLA) framework, this paper analyzes poverty alleviation measures as well as the impact of varied capital availability on sustainable livelihood. The study shows that developing local industries and governmental financial support improve the sustainable livelihood of farmers and eradicate absolute poverty. The findings of this study further indicate that there is a positive correlation between poverty alleviation measures and natural and social capital for sustainable livelihood. The paper provides empirical and quantitative evidence on alleviation of poverty, and the findings will help improve the sustainability of livelihood capability of farming households. This study suggests impactful approaches to stabilizing mechanisms for poverty alleviation in rural areas over the longer term.
The Combined Effect of Nitrogen Treatment and Weather Conditions on Wheat Protein-Starch Interaction and Dough Quality
Authors: Keres Indrek , Alaru Maarika , Koppel Reine , Altosaar Illimar , Tosens Tiina , Loit Evelin
Published: December 2021

The objective of this field crop study was to compare the effect of organic (cattle manure, off-season cover crop) and mineral N (NH4NO3; 0, 50, 100° 150 kg N ha−1) fertilizers on (i) gluten-starch interaction, and (ii) rheological properties of winter wheat dough. Data were collected from the long-term field experiment located in the Baltic Sea region (58°22′ N, 26°40′ E) in years 2013–2017. The amount of minuppueral N 150 kg ha−1 applied in two parts before flowering ensured higher gluten content (31 ± 3.3%) and dough quality (81 ± 7.4 mm) due to more positive interactions between gluten proteins and starch granules. The quality of dough was more variable in organic treatments (ranged up to 33%) because the availability of organic N was more variable and sensitivity to the weather conditions was higher. The mean variability of different dough properties over trial years under organic treatments was 1.4–2.0 times higher than in the treatment with 150 kg N ha−1.
Effects of Long-Term Straw Management and Potassium Fertilization on Crop Yield, Soil Properties, and Microbial Community in a Rice–Oilseed Rape Rotation
Authors: Li Jifu , Gan Guoyu , Chen Xi , Zou Jialong
Published: December 2021

The present study aims to assess the influences of long-term crop straw returning and recommended potassium fertilization on the dynamic change in rice and oilseed rape yield, soil properties, bacterial and fungal alpha diversity, and community composition in a rice–oilseed rape system. A long-term (2011–2020) field experiment was carried out in a selected paddy soil farmland in Jianghan Plain, central China. There were four treatments with three replications: NP, NPK, NPS, and NPKS, where nitrogen (N), phosphate (P), potassium (K), and (S) denote N fertilizer, P fertilizer, K fertilizer, and crop straw, respectively. Results showed that long-term K fertilization and crop straw returning could increase the crop yield at varying degrees for ten years. Compared with the NP treatment, the long-term crop straw incorporation with K fertilizer (NPKS treatment) was found to have the best effect, and the yield rates increased by 23.0% and 20.5% for rice and oilseed rape, respectively. The application of NPK fertilizer for ten years decreased the bacterial and fungal alpha diversity and the relative abundance of dominant bacterial and fungal taxa, whereas continuous straw incorporation had a contradictory effect. NPKS treatment significantly increased the relative abundance of some copiotrophic bacteria (Firmicutes, Gemmatimonadetes, and Proteobacteria) and fungi (Ascomycota). Available K, soil organic matter, dissolved organic carbon, and easily oxidized organic carbon were closely related to alterations in the composition of the dominant bacterial community; easily oxidized organic carbon, dissolved organic carbon, and slowly available K were significantly correlated with the fungal community. We conclude that long-term crop straw returning to the field accompanied with K fertilizer should be employed in rice-growing regions to achieve not only higher crop yield but also the increase in soil active organic carbon and available K content and the improvement of the biological quality of farmland.
The FBA Motif-Containing Protein NpFBA1 Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco
Authors: Xie Zhongyi , Wen Guo , Yang Yao , Wang Haiyan , Wang Jinying , Lei Chenggong , Guo Qigao , Dang Jiangbo , Liang Guolu
Published: December 2021

Plant leaf morphology has a great impact on plant drought resistance, ornamental research and leaf yield. In this study, we identified a new gene in Nicotiana plumbaginifolia, NpFBA1, that causes leaf curl. The results show that the NpFBA1 protein contains only one unique F-box associated (FBA) domain and does not have an F-box conserved domain. Phylogenetic analysis placed this gene and other Nicotiana FBA genes on a separate branch, and the NpFBA1 protein localized to the nucleus and cytoplasm. The expression of NpFBA1 was induced by black shank pathogen (Phytophthora parasitica var. nicotianae) infection and treatment with salicylic acid (SA) and methyl jasmonate (MeJA). NpFBA1-overexpressing transgenic lines showed leaf curling and aging during the rosette phase. During the bolting period, the leaves were curly and rounded, and the plants were dwarfed. In addition, NpFBA1-overexpressing lines were more susceptible to disease than wild-type (WT) plants. Further studies revealed that overexpression of NpFBA1 significantly downregulated the expression of auxin response factors such as NtARF3 and the lignin synthesis genes NtPAL, NtC4H, NtCAD2, and NtCCR1 in the leaves. In conclusion, NpFBA1 may play a key role in regulating leaf development and the response to pathogen infection.
Soil Quality Restoration during the Natural Succession of Abandoned Cattle Pastures in Deforested Landscapes in the Colombian Amazon
Authors: Rodríguez-León Carlos H. , Peña-Venegas Clara P. , Sterling Armando , Castro Daniel , Mahecha-Virguez Lizeth K. , Virguez-Díaz Yeny R. , Silva-Olaya Adriana M.
Published: December 2021

Successional processes in abandoned pastures in the Amazon region have been well-documented for the floristic component; however, soil succession has been poorly studied. This study assessed the physical, chemical and biological responses of soils in the Amazon region during the natural succession process in two main landscapes of the Colombian Amazon. Soil data on soil physico–chemical (bulk density, macroaggregates, pH and minerals) and biological (soil macrofauna) composition were evaluated along chronosequence with four successional stages: (i) degraded pastures, (ii) young (10–20-year-old), (iii) middle-age (25–40-year-old) and (iv) mature forests, in two different landscapes (hill and mountain). Individual soil variables and a synthetic indicator of soil quality (GISQ) were evaluated as tools for natural succession monitoring. The results corroborated the negative impact that cattle ranching has on Amazon soils. After 10 years of natural succession, the physico–chemical and biological soil components were widely restored. Less soil compaction and organic carbon occurred in older successional stages. Soil macrofauna richness and density increased along the chronosequence, with an evident association between the macrofauna composition and the macroaggregates in the soil. None of the individual soil properties or the GISQ indicator discriminated among natural succession stages; therefore, new soil quality indicators should be developed to monitor soil quality restoration in natural successions.
Sensor-Based Irrigation Reduces Water Consumption without Compromising Yield and Postharvest Quality of Soilless Green Bean
Authors: Palumbo Michela , D’Imperio Massimiliano , Tucci Vincenzo , Cefola Maria , Pace Bernardo , Santamaria Pietro , Parente Angelo , Montesano Francesco Fabiano
Published: December 2021

Real-time monitoring of substrate parameters in the root-zone through dielectric sensors is considered a promising and feasible approach for precision irrigation and fertilization management of greenhouse soilless vegetable crops. This research investigates the effects of timer-based (TIMER) compared with dielectric sensor-based irrigation management with different irrigation set-points [SENSOR_0.35, SENSOR_0.30 and SENSOR_0.25, corresponding to substrate volumetric water contents (VWC) of 0.35, 0.30 and 0.25 m3 m−3, respectively] on water use, crop performance, plant growth and physiology, product quality and post-harvest parameters of soilless green bean (Phaseolus vulgaris L., cv Maestrale). In SENSOR treatments, an automatic system managed irrigation in order to maintain substrate moisture constantly close to the specific irrigation set-point. The highest water amount was used in TIMER treatment, with a water saving of roughly 36%, 41% and 47% in SENSOR_0.35, SENSOR_0.30 and SENSOR_0.25, respectively. In TIMER, the leaching rate was ≈31% of the total water consumption, while little leaching (<10%) was observed in SENSOR treatments. TIMER and SENSOR_0.35 resulted in similar plant growth and yield, while irrigation set-points corresponding to lower VWC values (SENSOR_0.30 and SENSOR_0.25) resulted in inadequate water availability conditions and impaired the crop performance. The study confirms that rational sensor-based irrigation allows to save water without compromising anyhow the product quality. In SENSOR irrigation management, in fact, especially in the case of optimal water availability conditions, it was possible to obtain high quality pods, with fully satisfactory characteristics during storage at 7 °C for 15 days.
Deficit Water Irrigation in an Almond Orchard Can Reduce Pest Damage
Authors: González-Zamora José Enrique , Ruiz-Aranda Cristina , Rebollo-Valera María , Rodríguez-Morales Juan M. , Gutiérrez-Jiménez Salvador
Published: December 2021

Irrigated almond orchards in Spain are increasing in acreage, and it is pertinent to study the effect of deficit irrigation on the presence of pests, plant damage, and other arthropod communities. In an orchard examined from 2017 to 2020, arthropods and diseases were studied by visual sampling under two irrigation treatments (T1, control and T2, regulated deficit irrigation (RDI)). Univariate analysis showed no influence of irrigation on the aphid Hyalopterus amygdali (Blanchard) (Hemiptera: Aphididae) population and damage, but Tetranychus urticae Koch (Trombidiformes: Tetranychidae) damage on leaves was significantly less (50–60% reduction in damaged leaf area) in the T2 RDI treatment compared to the full irrigation T1 control in 2019 and 2020. Typhlocybinae (principal species Asymmetrasca decedens (Paoli) (Hemiptera: Cicadellidae)) population was also significantly lower under T2 RDI treatment. Chrysopidae and Phytoseiidae, important groups in the biological control of pests, were not affected by irrigation treatment. The most important diseases observed in the orchard were not, in general, affected by irrigation treatment. The multivariate principal response curves show significant differences between irrigation strategies in 2019 and 2020. In conclusion, irrigation schemes with restricted water use (such as T2 RDI) can help reduce the foliar damage of important pests and the abundance of other secondary pests in almond orchards.
Allergen-Specific IgE and IgG4 as Biomarkers for Immunologic Changes during Subcutaneous Allergen Immunotherapy
Authors: Nikolov Georgi , Todordova Yana , Emilova Radoslava , Hristova Diana , Nikolova Maria , Petrunov Bogdan
Published: December 2021

(1) Background: Biomarkers of efficacy for subcutaneous immunotherapy (SCIT) on allergic rhinitis have not been evaluated in details. The present study aims to assess the relevance of measuring of sIgE, sIgG4 and IgE/IgG4 ratio during SCIT in patients with allergic rhinitis; (2) Methods: 20 patients, 13 men and 7 women aged 19 to 58 years, with clinically manifested seasonal and perennial allergic rhinitis were studied. At the initiation and in the end of the three-year course of SCIT serum allergen-specific IgE and IgG4 were measured with ImmunoCAP system. The sIgE/sIgG4 ratio was calculated as a biomarker for immunologic effectiveness; (3) Results: There was a significant increase of sIgG4 antibodies (p < 0.05), while at the end of SCIT for the sIgE levels no significant changes were seen (p > 0.05). Moreover, 90% of patients showed a decrease of the IgE/IgG4 ratio; (4) Conclusions: In most of treated patients with AR, SCIT with Bulgarian allergen products leads to clear immunological changes. After a 3-year of SCIT there is a significant increase in allergen specific IgG4 levels and both decrease of sIgE and IgE/IgG4 ratio. sIgE, sIgG4 and IgE/IgG4 ratio can be used as a substantial biomarker for predicting immunological effectiveness of SCIT.
Characterization and Molecular Determinants for β-Lactam Specificity of the Multidrug Efflux Pump AcrD from Salmonella typhimurium
Authors: Cuesta Bernal Jenifer , El-Delik Jasmin , Göttig Stephan , Pos Klaas M.
Published: December 2021

Gram-negative Tripartite Resistance Nodulation and cell Division (RND) superfamily efflux pumps confer various functions, including multidrug and bile salt resistance, quorum-sensing, virulence and can influence the rate of mutations on the chromosome. Multidrug RND efflux systems are often characterized by a wide substrate specificity. Similarly to many other RND efflux pump systems, AcrAD-TolC confers resistance toward SDS, novobiocin and deoxycholate. In contrast to the other pumps, however, it in addition confers resistance against aminoglycosides and dianionic β-lactams, such as sulbenicillin, aztreonam and carbenicillin. Here, we could show that AcrD from Salmonella typhimurium confers resistance toward several hitherto unreported AcrD substrates such as temocillin, dicloxacillin, cefazolin and fusidic acid. In order to address the molecular determinants of the S. typhimurium AcrD substrate specificity, we conducted substitution analyses in the putative access and deep binding pockets and in the TM1/TM2 groove region. The variants were tested in E. coli ΔacrBΔacrD against β-lactams oxacillin, carbenicillin, aztreonam and temocillin. Deep binding pocket variants N136A, D276A and Y327A; access pocket variant R625A; and variants with substitutions in the groove region between TM1 and TM2 conferred a sensitive phenotype and might, therefore, be involved in anionic β-lactam export. In contrast, lower susceptibilities were observed for E. coli cells harbouring deep binding pocket variants T139A, D176A, S180A, F609A, T611A and F627A and the TM1/TM2 groove variant I337A. This study provides the first insights of side chains involved in drug binding and transport for AcrD from S. typhimurium.
Salmonella in Pig Farms and on Pig Meat in Suriname
Authors: Butaye Patrick , Halliday-Simmonds Iona , Van Sauers Astrid
Published: December 2021

Salmonella is one of the most important food borne zoonotic pathogens. While mainly associated with poultry, it has also been associated with pigs. Compared to the high-income countries, there is much less known on the prevalence of Salmonella in low- and middle-income countries, especially in the Caribbean area. Therefore, we investigated the prevalence of Salmonella in pigs and pig meat in Suriname. A total of 53 farms and 53 meat samples were included, and Salmonella was isolated using standard protocols. Strains were subjected to whole genome sequencing. No Salmonella was found on pig meat. Five farms were found to be positive for Salmonella, and a total of eight different strains were obtained. Serotypes were S. Anatum (n = 1), S. Ohio (n = 2), a monophasic variant of S. Typhimurium (n = 3), one S. Brandenburg, and one S. Javaniana. The monophasic variant of S. Typhimurium belonged to the ST34 pandemic clone, and the three strains were very similar. A few resistance genes, located on mobile genetic elements, were found. Several plasmids were detected, though only one was carrying resistance genes. This is the first study on the prevalence of Salmonella in pigs in the Caribbean and that used whole genome sequencing for characterization. The strains were rather susceptible. Local comparison of similar serotypes showed a mainly clonal spread of certain serotypes.
Potential Solutions Using Bacteriophages against Antimicrobial Resistant Bacteria
Authors: Rahimi-Midani Aryan , Lee Seon-Woo , Choi Tae-Jin
Published: December 2021

Bacteriophages are viruses that specifically infect a bacterial host. They play a great role in the modern biotechnology and antibiotic-resistant microbe era. Since the discovery of phages, their application as a control agent has faced challenges that made antibiotics a better fit for combating pathogenic bacteria. Recently, with the novel sequencing technologies providing new insight into the nature of bacteriophages, their application has a second chance to be used. However, novel challenges need to be addressed to provide proper strategies for their practical application. This review focuses on addressing these challenges by initially introducing the nature of bacteriophages and describing the phage-host-dependent strategies for phage application. We also describe the effect of the long-term application of phages in natural environments and other bacterial communities. Overall, this review gathered crucial information for the future application of phages. We predict the use of phages will not be the only control strategy against pathogenic bacteria. Therefore, more studies must be done for low-risk control methods against antimicrobial-resistant bacteria.
Evaluation of Multidrug-Resistant P. aeruginosa in Healthcare Facility Water Systems
Authors: Cristina Maria Luisa , Sartini Marina , Schinca Elisa , Ottria Gianluca , Casini Beatrice , Spagnolo Anna Maria
Published: December 2021

According to the WHO, P. aeruginosa is one of the antibiotic-resistant bacteria that represent the biggest threat to public health. The aim of the study was to establish the prevalence of antibiotic-resistant P. aeruginosa in the water systems of various healthcare facilities over the course of nine years. A total of 4500 tap water system samples were taken from seventeen healthcare facilities. The culture method was used to detect P. aeruginosa, and the isolates were then tested for antibiotic resistance using the standardised disc diffusion method. Eleven antibiotics from five different classes were tested. P. aeruginosa was found to have contaminated 2.07% (no. 93) of the water samples. The majority of positive samples came from the dental units (30.11%) and the ward kitchens (23.66%). Considering the total isolates, 56.99% (no. 3) were resistant to at least one of the antibiotics tested. A total of 71.43% of P. aeruginosa isolated from water emerging from dental unit handpieces was antibiotic-resistant, with 45% of it resistant to ≥3 classes of antibiotics. Out of the total isolates, 19.35% showed resistance to carbapenems. It would be advisable to systematically screen tap water for opportunistic micro-organisms such as P. aeruginosa, as many countries already do, including this in the Water Safety Plan.
Efflux Pump Mediated Antimicrobial Resistance by Staphylococci in Health-Related Environments: Challenges and the Quest for Inhibition
Authors: Dashtbani-Roozbehani Abolfazl , Brown Melissa H.
Published: December 2021

The increasing emergence of antimicrobial resistance in staphylococcal bacteria is a major health threat worldwide due to significant morbidity and mortality resulting from their associated hospital- or community-acquired infections. Dramatic decrease in the discovery of new antibiotics from the pharmaceutical industry coupled with increased use of sanitisers and disinfectants due to the ongoing COVID-19 pandemic can further aggravate the problem of antimicrobial resistance. Staphylococci utilise multiple mechanisms to circumvent the effects of antimicrobials. One of these resistance mechanisms is the export of antimicrobial agents through the activity of membrane-embedded multidrug efflux pump proteins. The use of efflux pump inhibitors in combination with currently approved antimicrobials is a promising strategy to potentiate their clinical efficacy against resistant strains of staphylococci, and simultaneously reduce the selection of resistant mutants. This review presents an overview of the current knowledge of staphylococcal efflux pumps, discusses their clinical impact, and summarises compounds found in the last decade from plant and synthetic origin that have the potential to be used as adjuvants to antibiotic therapy against multidrug resistant staphylococci. Critically, future high-resolution structures of staphylococcal efflux pumps could aid in design and development of safer, more target-specific and highly potent efflux pump inhibitors to progress into clinical use.
Mito-TIPTP Increases Mitochondrial Function by Repressing the Rubicon-p22phox Interaction in Colitis-Induced Mice
Authors: Kim Jae-Sung , Kim Ye-Ram , Jang Sein , Wang Sang Geon , Cho Euni , Mun Seok-Jun , Jeon Hye-In , Kim Hyo-Keun , Min Sun-Joon , Yang Chul-Su
Published: December 2021

The run/cysteine-rich-domain-containing Beclin1-interacting autophagy protein (Rubicon) is essential for the regulation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase by interacting with p22phox to trigger the production of reactive oxygen species (ROS) in immune cells. In a previous study, we demonstrated that the interaction of Rubicon with p22phox increases cellular ROS levels. The correlation between Rubicon and mitochondrial ROS (mtROS) is poorly understood. Here, we report that Rubicon interacts with p22phox in the outer mitochondrial membrane in macrophages and patients with human ulcerative colitis. Upon lipopolysaccharide (LPS) activation, the binding of Rubicon to p22phox was elevated, and increased not only cellular ROS levels but also mtROS, with an impairment of mitochondrial complex III and mitochondrial biogenesis in macrophages. Furthermore, increased Rubicon decreases mitochondrial metabolic flux in macrophages. Mito-TIPTP, which is a p22phox inhibitor containing a mitochondrial translocation signal, enhances mitochondrial function by inhibiting the association between Rubicon and p22phox in LPS-primed bone-marrow-derived macrophages (BMDMs) treated with adenosine triphosphate (ATP) or dextran sulfate sodium (DSS). Remarkably, Mito-TIPTP exhibited a therapeutic effect by decreasing mtROS in DSS-induced acute or chronic colitis mouse models. Thus, our findings suggest that Mito-TIPTP is a potential therapeutic agent for colitis by inhibiting the interaction between Rubicon and p22phox to recover mitochondrial function.
Redox Systems, Oxidative Stress, and Antioxidant Defences in Health and Disease
Authors: Allegra Mario
Published: December 2021

Reactive oxygen and nitrogen species (RONS) play a key role in the regulation of cell survival [...]
Friend or Foe? The Roles of Antioxidants in Acute Lung Injury
Authors: Liu Yang , Zhou Shujun , Xiang Du , Ju Lingao , Shen Dexin , Wang Xinghuan , Wang Yanfeng
Published: December 2021

Acute lung injury (ALI) is an acute hypoxic respiratory insufficiency caused by various intra- and extra-pulmonary injury factors. The oxidative stress caused by excessive reactive oxygen species (ROS) produced in the lungs plays an important role in the pathogenesis of ALI. ROS is a “double-edged sword”, which is widely involved in signal transduction and the life process of cells at a physiological concentration. However, excessive ROS can cause mitochondrial oxidative stress, leading to the occurrence of various diseases. It is well-known that antioxidants can alleviate ALI by scavenging ROS. Nevertheless, more and more studies found that antioxidants have no significant effect on severe organ injury, and may even aggravate organ injury and reduce the survival rate of patients. Our study introduces the application of antioxidants in ALI, and explore the mechanisms of antioxidants failure in various diseases including it.
Antibacterial, Antifungal, and Antioxidant Activities of Silver Nanoparticles Biosynthesized from Bauhinia tomentosa Linn
Authors: Renganathan Senthil , Subramaniyan Sugunakala , Karunanithi Nivetha , Vasanthakumar Preethi , Kutzner Arne , Kim Pok-Son , Heese Klaus
Published: December 2021

The biogenic synthesis of silver nanoparticles (AgNPs) has a wide range of applications in the pharmaceutical industry. Here, we synthesized AgNPs using the aqueous flower extract of Bauhinia tomentosa Linn. Formation of AgNPs was observed using ultraviolet-visible light spectrophotometry at different time intervals. Maximum absorption was observed after 4 h at 420 nm due to the reduction of Ag+ to Ag0. The stabilizing activity of functional groups was identified by Fourier-transform infrared spectroscopy. Size and surface morphology were also analyzed using scanning electron microscopy. The present study revealed the AgNPs were spherical in form with a diameter of 32 nm. The face-centered cubic structure of AgNPs was indexed using X-ray powder diffraction with peaks at 2θ = 37°, 49°, 63°, and 76° (corresponding to the planes of silver 111, 200, 220, 311), respectively. Energy-dispersive X-ray spectroscopy revealed that pure reduced silver (Ag0) was the major constituent (59.08%). Antimicrobial analyses showed that the biosynthesized AgNPs possess increased antibacterial activity (against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative), with larger zone formation against S. aureus (9.25 mm) compared with that of E. coli (6.75 mm)) and antifungal activity (against Aspergillus flavus and Candida albican (with superior inhibition against A. flavus (zone of inhibition: 7 mm) compared with C. albicans (zone of inhibition: 5.75 mm)). Inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was found to be dose-dependent with half-maximal inhibitory concentration (IC50) values of 56.77 μg/mL and 43.03 μg/mL for AgNPs and ascorbic acid (control), respectively, thus confirming that silver nanoparticles have greater antioxidant activity than ascorbic acid. Molecular docking was used to determine the mode of antimicrobial interaction of our biosynthesized B. tomentosa Linn flower-powder extract-derived AgNPs. The biogenic AgNPs preferred hydrophobic contacts to inhibit bacterial and fungal sustainability with reducing antioxidant properties, suggesting that biogenic AgNPs can serve as effective medicinal agents.
Hibiscus Flower and Olive Leaf Extracts Activate Apoptosis in SH-SY5Y Cells
Authors: Chiaino Elda , Micucci Matteo , Budriesi Roberta , Mattioli Laura Beatrice , Marzetti Carla , Corsini Maddalena , Frosini Maria
Published: December 2021

Compounds of natural origin may constitute an interesting tool for the treatment of neuroblastoma, the most prevalent extracranial solid tumor in children. PRES is a commercially available food supplement, composed of a 13:2 (w/w) extracts mix of Olea europaea L. leaves (OE) and Hibiscus sabdariffa L. flowers (HS). Its potential towards neuroblastoma is still unexplored and was thus investigated in human neuroblastoma SH-SY5Y cells. PRES decreased the viability of cells in a concentration-dependent fashion (24 h IC50 247.2 ± 31.8 µg/mL). Cytotoxicity was accompanied by an increase in early and late apoptotic cells (AV-PI assay) and sub G0/G1 cells (cell cycle analysis), ROS formation, reduction in mitochondrial membrane potential, and caspases activities. The ROS scavenger N-acetyl-L-cysteine reverted the cytotoxic effects of PRES, suggesting a key role played by ROS in PRES-mediated SH-SY5Y cell death. Finally, the effects of OE and HS extracts were singularly tested and compared to those of the corresponding mixture. OE- or HS-mediated cytotoxicity was always significantly lower than that caused by PRES, suggesting a synergic effect. In conclusion, the present findings highlight the potential of PRES for the treatment of neuroblastoma and offers the basis for a further characterization of the mechanisms underlying its effects.
Orthodontic Treatment for a Child with Melanotic Neuroectodermal Tumor during Infancy: A Case Report
Authors: Hichijo Natsuko , Noguchi Tadahide , Sasaguri Kenichi , Mori Yoshiyuki
Published: December 2021

Background: Melanotic neuroectodermal tumor of infancy (MNTI) is a very rare tumor, and case reports of orthodontic treatment for patients with MNTI may be previously unreported. This article describes the orthodontic treatment for a 7-year-old girl with MNTI in the mandible. Case: Her chief complaint was anterior crossbite. Although she had an MNTI diagnosis at the age of 8 months, it remained subclinical. Therefore, she has been regularly followed-up by computed tomography (CT) and magnetic resonance (MR) imaging without aggressive treatment. We had worried about the stimulation of MNTI on the mandible by changing her occlusal position with orthodontic treatment. Therefore, we sufficiently explained to her and her family that orthodontic treatment was at risk for worsening MNTI. However, they desired treatment that consisted of maxillary protraction and slow expansion to correct anterior crossbite and encourage the permanent tooth eruption. After 19 months of active orthodontic treatment, the anterior crossbite was improved, and the eruption of permanent teeth made good progress. No evidence of progression and exacerbation of MNTI has been found by both CT and MR imaging. As the observation period is still short, we need a careful and long-term follow-up of her occlusion and MNTI. Furthermore, when we encounter rare cases without previous experience and reports, informed consent was of particular importance.
Monitoring Brain State and Behavioral Performance during Repetitive Visual Stimulation
Authors: Kuc Alexander K. , Kurkin Semen A. , Maksimenko Vladimir A. , Pisarchik Alexander N. , Hramov Alexander E.
Published: December 2021

We tested whether changes in prestimulus neural activity predict behavioral performance (decision time and errors) during a prolonged visual task. The task was to classify ambiguous stimuli—Necker cubes; manipulating the degree of ambiguity from low ambiguity (LA) to high ambiguity (HA) changed the task difficulty. First, we assumed that the observer’s state changes over time, which leads to a change in the prestimulus brain activity. Second, we supposed that the prestimulus state produces a different effect on behavioral performance depending on the task demands. Monitoring behavioral responses, we revealed that the observer’s decision time decreased for both LA and HA stimuli during the task performance. The number of perceptual errors lowered for HA, but not for LA stimuli. EEG analysis revealed an increase in the prestimulus 9–11 Hz EEG power with task time. Finally, we found associations between the behavioral and neural estimates. The prestimulus EEG power negatively correlated with the decision time for LA stimuli and the erroneous responses rate for HA stimuli. The obtained results confirm that monitoring prestimulus EEG power enables predicting perceptual performance on the behavioral level. The observed different time-on-task effects on the LA and HA stimuli processing may shed light on the features of ambiguous perception.