bluuebunny/crossref_metadata_2025 huggingface dataset:
Fluorescence spectroscopy for tracking microbiological contamination in urban waterbodies
Authors: Angelotti de Ponte Rodrigues Natália , Carmigniani Rémi , Guillot-Le Goff Arthur , Lucas Françoise S. , Therial Claire , Naloufi Manel , Janne Aurélie , Piccioni Francesco , Saad Mohamed , Dubois Philippe , Vinçon-Leite Brigitte
Published: May 2024

Dissolved organic matter (DOM) plays a crucial role in freshwater ecosystem function. Monitoring of DOM in aquatic environments can be achieved by using fluorescence spectroscopy. Particularly, DOM fluorescence can constitute a signature of microbiological contamination with a potential for high frequency monitoring. However, limited data are available regarding urban waterbodies. This study considers fluorescence data from field campaigns conducted in the Paris metropolitan region: two watercourses (La Villette basin and the river Marne), two stormwater network outlets (SO), and a wastewater treatment plant effluent (WWTP-O). The objectives of the study were to characterize the major fluorescence components in the studied sites, to investigate the impact of local rainfall in such components and to identify a potential fluorescence signature of local microbiological contamination. The components of a PARAFAC model (C1-C7), corresponding to a couple of excitation (ex) and emission (em) wavelengths, and the fluorescence indices HIX and BIX were used for DOM characterization. In parallel, fecal indicator bacteria (FIB) were measured in selected samples. The PARAFAC protein-like components, C6 (ex/em of 280/352 nm) and C7 (ex/em of 305/340 nm), were identified as markers of microbial contamination in the studied sites. In the La Villette basin, where samplings covered a period of more than 2 years, which also included similar numbers of wet and dry weather samples, the protein-like components were significantly higher in wet weather in comparison to dry weather. A positive relationship was obtained between C6 and FIB. In urban rivers, the high frequency monitoring of C6 levels would support the fecal contamination detection in rivers. In addition, it could help targeting specific field campaigns to collect comprehensive dataset of microbiological contamination episodes.
The electron density at the midpoint of the plasmapause
Authors: Denton Richard E. , Tengdin Phoebe M. , Hartley David P. , Goldstein Jerry , Lee Jinmyoung , Takahashi Kazue
Published: May 2024

The high density plasmasphere in the magnetosphere is often separated from a lower density region outside of the plasmasphere, called the plasmatrough, by a sharp gradient in electron density called the plasmapause. Here we use plasmapause events identified from electron density data from the ISEE, CRRES, Polar, and IMAGE missions, and the nonlinear genetic algorithm TuringBot, to find models for the electron density at the midpoint of the plasmapause, ne,pp. A good model for ne,pp should include dependence on L, which is the strongest dependence. But models can be improved by including weaker dependencies on the magnetic local time, MLT, the solar EUV index F10.7, and geomagnetic activity as indicated by averages of Kp and AE. The most complicated model that we present predicts ne,pp within a factor of 1.64, and is within the range of observed plasmapause densities for about 96% of our events. These models can be useful for separating plasma populations into plasmasphere-like and plasmatrough-like populations. We also make available our database of electron density measurements categorized into various populations.
Case report: Abdominal mesothelioma in Atelerix albiventris
Authors: Troncoso Ignacio , Brown John , Isla Carla , Lajara Juan Manuel , Ebner Max , Fehrmann-Cartes Karen
Published: May 2024

Hedgehogs, as exotic species, are more susceptible to various neoplastic conditions affecting diverse bodily systems, particularly the tegumentary, hemolymphatic, and digestive systems. Among these conditions, epithelial tumors are the most prevalent, followed by round cell tumors and mesenchymal tumors. A striking characteristic is the malignant nature of over 8% of these tumors, leading to a generally unfavorable prognosis. This study aims to present a unique case involving a 2.5 year-old male African pygmy hedgehog in Concepción, Biobío District, Chile, diagnosed with a mesenchymal neoplasia originating from mesothelial cells. The hedgehog presented to the veterinary clinic with acute abdominal pain, prompting ultrasound imaging, and comprehensive cytological, histopathological, and immunohistochemical analyses. During abdominal ultrasound, a mass was observed, and its cytological examination revealed the presence of malignant cells. The histopathological examination unveiled a diffuse mesothelial cell tissue interwoven with abundant fibrous tissue and small cysts containing serous fluid, all enveloped by flattened or cuboidal cells of mesothelial origin. Immunohistochemistry further confirmed the diagnosis, demonstrating positive immunostaining for calretinin and mesothelin markers, corroborating the diagnosis of fibrous malignant peritoneal mesothelioma. This case highlights the complexity of neoplastic conditions in hedgehogs and emphasizes the importance of multimodal diagnostic approaches for accurate identification and understanding of these rare diseases.
Recombinant linear multiple epitopes of σB protein protect Muscovy ducks against novel duck reovirus infection
Authors: Chen Yiquan , Yan Zhuanqiang , Liao Changtao , Song Yiwei , Zhou Qi , Shen Hanqin , Chen Feng
Published: May 2024

Infection by the novel duck reovirus (NDRV) in ducklings causes high mortality, which leads to substantial economic losses in the duck industry in China. To date, no commercial vaccine is available for this disease. In this study, linear B cell epitopes of the σB protein of the NDRV were predicted and recombinant multiple linear B cell epitopes (MLBEs) were constructed through linkers. The recombinant MLBEs were then expressed and purified. One-day-old Muscovy ducklings were immunized with different doses of MLBEs and challenged with 5 × 104 ELD50 of the virulent CHY strain of NDRV 14 days after immunization. The ducklings vaccinated with 20 and 40 μg of MLBE performed no clinical signs or gross or histopathological lesions, indicating 100% protection against infection. The viral load in the liver and spleens of these birds was significantly lower than that in the control group. Additionally, these ducklings exhibited positive seroconversion at 7 days after vaccination on enzyme-linked immunosorbent assay. These results indicate that MLBE of σB could be used as a candidate for developing vaccines against NDRV infection.
MicroRNAs: exploring their role in farm animal disease and mycotoxin challenges
Authors: Kappari Laharika , Dasireddy Joseph Rishitha , Applegate Todd J. , Selvaraj Ramesh K. , Shanmugasundaram Revathi
Published: May 2024

MicroRNAs (miRNAs) serve as key regulators in gene expression and play a crucial role in immune responses, holding a significant promise for diagnosing and managing diseases in farm animals. This review article summarizes current research on the role of miRNAs in various farm animal diseases and mycotoxicosis, highlighting their potential as biomarkers and using them for mitigation strategies. Through an extensive literature review, we focused on the impact of miRNAs in the pathogenesis of several farm animal diseases, including viral and bacterial infections and mycotoxicosis. They regulate gene expression by inducing mRNA deadenylation, decay, or translational inhibition, significantly impacting cellular processes and protein synthesis. The research revealed specific miRNAs associated with the diseases; for instance, gga-miR-M4 is crucial in Marek’s disease, and gga-miR-375 tumor-suppressing function in Avian Leukosis. In swine disease such as Porcine Respiratory and Reproductive Syndrome (PRRS) and swine influenza, miRNAs like miR-155 and miR-21-3p emerged as key regulatory factors. Additionally, our review highlighted the interaction between miRNAs and mycotoxins, suggesting miRNAs can be used as a biomarker for mycotoxin exposure. For example, alterations in miRNA expression, such as the dysregulation observed in response to Aflatoxin B1 (AFB1) in chickens, may indicate potential mechanisms for toxin-induced changes in lipid metabolism leading to liver damage. Our findings highlight miRNAs potential for early disease detection and intervention in farm animal disease management, potentially reducing significant economic losses in agriculture. With only a fraction of miRNAs functionally characterized in farm animals, this review underlines more focused research on specific miRNAs altered in distinct diseases, using advanced technologies like CRISPR-Cas9 screening, single-cell sequencing, and integrated multi-omics approaches. Identifying specific miRNA targets offers a novel pathway for early disease detection and the development of mitigation strategies against mycotoxin exposure in farm animals.
The Effect of Arm Movements on the Dynamics of the Wheelchair Frame during Manual Wheelchair Actuation and Propulsion
Authors: Fuss Franz , Tan Adin , Weizman Yehuda
Published: May 2024

Wheelchair propulsion and actuation are influenced by the moving masses of the wheelchair user; however, the extent of this effect is still unclear. The main evidence of this effect is that the speed of the wheelchair frame continues to increase after the end of the push phase. The wheelchair’s speed was measured using IMUs and the duration of the push period was recorded using miniaturised pressure sensors attached to the driver’s middle fingers. The velocity and acceleration were determined for various average stroke cycle speeds to determine the speed dependency of the acceleration. The wheelchair was then mounted on a force plate to measure the inertial forces of the hands moving back and forth. The aerodynamic drag and rolling resistance forces were determined from coast-down experiments. Based on the measured forces, the behaviour of the force and velocity profiles was finally modelled by gradually reducing the mass of the arms and thus their inertial force. The results showed that the wheelchair is accelerated throughout the push phase (except for a temporary deceleration in the middle of the push phase at higher velocities), and that this acceleration continues well after the push phase. In the second half of the recovery phase, the wheelchair decelerates. The horizontal inertial forces measured on the force plate are predominantly negative in the push phase and in the second half of the recovery phase, and positive in the first half of the push phase, and their impulse is zero due to the conservation of momentum. Modelling the wheelchair with moving masses showed that reducing the horizontal inertial forces has no effect on the driver’s propulsive force but reduces the velocity fluctuations. The main conclusion of this research is that the wheelchair user’s power should be calculated only from the pure propulsive force that is required in the push phase to overcome the dissipative forces and that enables the gain or loss in speed per stroke cycle, but not directly from the measured velocity.
Changes in Attitude toward Intimate Partner Violence in Rapidly Developing Countries: The Case of Indonesia
Authors: Noda Moemi , Ishida Akira
Published: May 2024

Male-perpetrated intimate partner violence (IPV) is a severe human rights violation that negatively affects women’s well-being worldwide. Although many studies have examined the factors influencing IPV, few have investigated the changes in attitudes toward IPV during rapid economic growth. Therefore, this study aimed to clarify changes in attitudes toward husband-on-wife violence by gender, from 2007 to 2017, using individual data from the Indonesia Demographic and Health Surveys. The estimation results revealed that, despite being more accepting of IPV, young women, women living in rural areas other than Java and Bali, and women belonging to lower social classes have significantly increased their negative attitudes toward IPV over the past decade. Although negative attitudes toward IPV have increased significantly among men living in eastern Indonesia, men in their teens, 20s, and 30s and those living in Sumatra have become more accepting of IPV. This suggests that the overall awareness of IPV resistance among men has not increased. The acceptance of IPV is more prevalent among employed women in the middle and lower socioeconomic strata than among their unemployed counterparts. However, the reverse trend has become clearer among women in the upper strata over the past decade.
Experimental Investigation of Impulsive Coupling Characteristics of Asteroid Simulants Based on Laser Ablation Propulsion
Authors: Ma Yingjie , Chang Hao , Zhou Weijing , Jian Zhilong
Published: May 2024

The ablation impulse of typical asteroid simulants irradiated by a nanosecond pulsed laser has been investigated in a vacuum environment. A torsional pendulum measurement system was constructed to calculate the impulse of laser ablation. A 10 ns pulsed laser was used, with a 1064 nm wavelength, a 900 mJ maximum pulse energy, and a millimeter-scale ablation spot diameter. Impulsive coupling characteristics of six typical targets that imitate the substance of asteroids with various laser fluences were analyzed. Furthermore, the impulse coupling coefficient curves of different materials were fitted. The results reveal that the minimum laser fluence corresponding to a measurable ablation impulse is approximately 2.5 J/cm2, and the optimum laser fluence corresponding to the maximum impulse coupling coefficient is approximately 14.0 J/cm2. The trends of the laser ablation impulse coupling curves are roughly consistent for the six materials. Impulse coupling characteristics of the six typical materials can be represented by the same polynomial within a 95% confidence interval, so a unified rule has been given. In actual deflection tasks of asteroids, the unified impulse coupling characteristic can be used to implement laser deflection techniques, especially when the material of the asteroid cannot be accurately judged in time.
Soil Amendment Combining Bentonite and Maize Straw Improves Soil Quality Cropped to Oat in a Semi-Arid Region
Authors: Zhang Lanying , Mi Junzhen , Zhao Baoping , Cui Xuemei , Hu Kexin , McLaughlin Neil B. , Liu Jinghui
Published: May 2024

Soil amendments have been proposed as an effective way to enhance soil carbon stocks on degraded soils, particularly in dryland farming areas. Soil organic carbon (SOC) plays an important role in improving soil quality, and soil aggregates are known to be crucial in sequestering and protecting SOC. However, how aggregation and protection of SOC by aggregates respond to a single application of bentonite combined with maize straw remains unknown, especially in the sandy soil of a semi-arid region. A three-year field experiment with four treatments [no amendment (CK), maize straw amendment addition only (T1, 6 Mg ha−1), bentonite amendment addition only (T2, 18 Mg ha−1), and maize straw combined with bentonite amendment (T3, 6 Mg ha−1 maize straw plus 18 Mg ha−1 bentonite)] was conducted in the Loess Plateau of China to assess the effects of bentonite and maize straw on aggregation and SOC. The results indicated that soil bulk density decreased by 2.72–5.42%, and soil porosity increased by 3.38–8.77% with three years of T3 application, especially in the 20–40 cm layer, compared with CK. T3 increased the amount of C input, SOC stock, and SOC stock sequestration rate by 1.04 Mg ha−1 y−1, 0.84–1.08 Mg ha−1, and 0.49 Mg ha−1 y−1, respectively, and it increased the mass proportions and aggregate-associated C stock of >0.25 mm aggregates by 1.15–2.51- and 1.59–2.96-fold compared with CK. Correlation analysis showed a positive correlation of total SOC stock with the C concentration of >2 mm, 0.25–2 mm, and 0.053–0.25 mm aggregates. Aggregates of various sizes in sandy soils have the potential for greater SOC stock. Our findings suggest that the application of maize straw (6 Mg ha−1) combined with bentonite (18 Mg ha−1) would be an effective management strategy to enhance the bulk soil C pools by improving the soil structure and thereby improving soil fertility.
Effects of Microbial Inoculants Combined with Chemical Fertilizer on Growth and Soil Nutrient Dynamics of Timothy (Phleum pratense L.)
Authors: He Shanmu , Zhang Ying , Yang Xiaolei , Li Qi , Li Changning , Yao Tuo
Published: May 2024

Microbial inoculants derived from plant growth-promoting rhizobacteria (PGPR) offer eco-friendly alternatives to traditional chemical fertilizers, maintaining microbiota balance in agricultural systems. However, limited research has explored the combined effects of microbial inoculants and chemical fertilizers on crop growth and soil properties. In this study, we investigated seven fertilizer combinations, ranging from no fertilizer to various proportions of chemical fertilizers with microbial inoculants, on timothy (Phleum pratense L.) growth, chlorophyll content, soil properties, enzyme activities, and soil microbial communities. A randomized block design was employed to analyze these effects. The results indicate that the combination of 85% chemical fertilizer with microbial inoculants significantly increased timothy yield and chlorophyll content. In addition, a reduction to 55% chemical fertilizer in conjunction with microbial inoculants resulted in comparable yield to that of 100% fertilizer with no inoculants. The microbial inoculants treatments notably elevated soil catalase, urease, acid phosphatase, and invertase activities, along with soil fast-acting nutrient content. The sequencing results show that the abundance of beneficial bacteria increased, while that of fungi decreased in the soil rhizosphere after the application of microbial inoculants. This study underscored the potential of microbial inoculants combined with reductions in chemical fertilizers to enhance soil microbiology, nutrient content, and beneficial microbial abundance while suppressing pathogenic fungi, thereby promoting timothy growth and yield. These findings provide a theoretical basis for the use of microbial inoculants in sustainable agricultural practices, providing valuable insights for optimizing microbial inoculants and chemical fertilizer formulations to mitigate the sustainability challenges posed by conventional fertilizers.
QTL Mapping and Candidate Gene Mining for Stem Diameter Using Genetic Basis of Cultivated Soybean and Wild Soybean
Authors: Chen Lin , Li Fuxin , Li Lanxin , Ma Shengnan , Yu Lin , Tang Chunshuang , Zhao Kuangyu , Song Zhen , Liu Chunyan , Chen Qingshan , Wang Jinhui
Published: May 2024

Soybean (Glycine max) is a vital food crop, serving as a major source of high-quality protein for human and animal consumption. Stem diameter is one of the primary determinants of the stem lodging resistance of a given plant, but there has been relatively little research to date focused on genes associated with this trait. To address this gap in the literature, 207 chromosome segment substitution lines (CSSLs) were generated in the present study through the crossing and backcrossing of the improved Suinong14 and the wild ZYD00006 soybean varieties. These CSSLs were then used for the mapping of quantitative trait loci (QTLs) associated with stem diameter in two-year field planting materials, leading to the identification of nine QTLs. Whole genome resequencing, RNA-seq, and qPCR were then used to evaluate candidate genes associated with stem diameter within these QTL intervals, ultimately leading to the selection of Glyma.04G004100 as a stem diameter-related gene. Subsequent qPCR analyses revealed that Glyma.04g004100 was upregulated in soybean plants with larger stem diameters, and haplotype analyses yielded results consistent with these stem diameter data in the population used to conduct this study. In summary, a series of QTLs associated with stem diameter were identified in the present study, resulting in the establishment of Glyma.04g004100 as a stem diameter-related gene. Together, these results offer a theoretical foundation for the future molecular-assisted breeding of lodging-resistant soybean varieties, and future functional research focused on Glyma.04g004100 may elucidate the molecular mechanisms and key signaling networks involved in soybean stem development.
Exploring the Synergy between Humic Acid Substances, Dehydrogenase Activity and Soil Fertility
Authors: Kagan Katarzyna , Goraj Weronika , Kuźniar Agnieszka , Kruczyńska Anna , Sochaczewska Anna , Słomczewski Andrzej , Wolińska Agnieszka
Published: May 2024

The European Commission’s “Farm to Folk” (F2F) strategy recommends reducing fertilizers by at least 20% by 2030. In this aspect, the main goal of our study was to verify whether a 20 and even 40% reduction in nitrogen (N) fertilization rate would be sufficient to maintain soil fertility (expressed as dehydrogenase activity—DHA, humic acid substances—HA substances, E4/E6 ratio), and yields of wheat and rapeseed cultivated in strip-till technology. Two fields (10 ha each) were established for wheat and rapeseed cultivation, and soils (0–20 cm) were sampled before sowing and after harvesting. It was found that a 20% fertilization reduction does not adversely affect the biological activity expressed by DHA. Nevertheless, the reduction in nitrogen (N) fertilizer rates led to a slight decrease in the E4/E6 ratio, which could serve as a potential indicator of fertilization reduction. DHA, E4/E6 ratio and content of HA substances were also dependent on the crop. Following harvesting, both rapeseed and wheat exhibited a positive correlation between the E4/E6 ratio and the degree of fertilization rate (FR), as well as yield.
Establishment of Nested PCR for the Detection of Pseudomonas plecoglossicida and Epidemiological Survey of Larimichthys crocea in the Southeast Coastal Region
Authors: Duan Xinbing , Li Jiji , Shi Hui , Tao Zhen , Wei Xuelian , Ye Yingying , Guo Baoying
Published: May 2024

The visceral white nodules disease in the internal organs of Larimichthys crocea has caused significant harm in the aquaculture of this species, with Pseudomonas plecoglossicida considered one of the core pathogens causing this disease. In this study, we designed three pairs of specific nested PCR primers targeting the sctU gene of P. plecoglossicida, a crucial component of the Type III secretion system (T3SS), which is instrumental in bacterial pathogenesis and virulence. Through the optimization of PCR reaction conditions, specificity testing, and sensitivity determination, a method was established for the accurate detection of P. plecoglossicida. This method yielded single amplification products, exhibited a false positive rate of zero for reference bacteria, and achieved a detection sensitivity of a minimum of 2.62 copies/reaction for the target sequence. Using the detection method, we conducted analyses on the diseased populations of L. crocea, involving a total of 64 screened fishes along the southeast coast of China from 2021 to 2023. The results revealed that the infection rate of P. plecoglossicida in diseased L. crocea exceeded over 90% in March and April, while in other months, the maximum recorded infection rate was merely 10%. The detection method developed in this study shows potential for early warning and routine monitoring of visceral white nodules disease in the internal organs of species such as L. crocea.
Surveillance of Antimicrobial Resistance of Escherichia coli Isolates from Intestinal Contents of Dairy and Veal Calves in the Veneto Region, Northeaster Italy
Authors: Bortolami Laura , Barberio Antonio , Schiavon Eliana , Martignago Federico , Littamè Erica , Sturaro Anna , Gagliazzo Laura , De Lucia Alessia , Ostanello Fabio
Published: May 2024

This surveillance study aimed to estimate the proportion of antimicrobial resistant strains and antimicrobial resistance (AMR) profiles of E. coli isolates detected from the intestinal contents of veal and dairy calves in the Veneto Region, Northeaster Italy. Additionally, we investigated the differences in AMR profiles between dairy and veal calves over the period 2017–2022. Overall 1150 E. coli isolates were tested from calves exhibiting enteric disease, with 868 from dairy and 282 from veal calves. The percentage of resistant isolates to nine antimicrobials was notably higher in veal calves compared to dairy calves, except for ampicillin. Throughout the study period, we observed a significant increase in the proportion of resistant isolates to florfenicol, gentamycin, paromomycin, tetracycline and trimethoprim/sulfamethoxazole in dairy calves, while we did not detect any significant increase in the proportion of resistant isolates among veal calves. A substantial proportion (75.9%) of the isolated E. coli exhibited multi-drug resistance (MDR). The proportion of multi-drug resistant isolates was significantly higher in veal calves (91.7%) compared to dairy calves (74.3%) all through the surveillance period (2017–2022), with no significant variation in MDR proportion among veal calves between 2017 and 2022 but a significant increase among dairy calves.
Different Evolutionary Trends of Galloanseres: Mitogenomics Analysis
Authors: Zhou Shengyang , Wang Xibao , Wang Lidong , Gao Xiaodong , Lyu Tianshu , Xia Tian , Shi Lupeng , Dong Yuehuan , Mei Xuesong , Zhang Zhihao , Zhang Honghai
Published: May 2024

The two existing clades of Galloanseres, orders Galliformes (landfowl) and Anseriformes (waterfowl), exhibit dramatically different evolutionary trends. Mitochondria serve as primary sites for energy production in organisms, and numerous studies have revealed their role in biological evolution and ecological adaptation. We assembled the complete mitogenome sequences of two species of the genus Aythya within Anseriformes: Aythya baeri and Aythya marila. A phylogenetic tree was constructed for 142 species within Galloanseres, and their divergence times were inferred. The divergence between Galliformes and Anseriformes occurred ~79.62 million years ago (Mya), followed by rapid evolution and diversification after the Middle Miocene (~13.82 Mya). The analysis of selective pressure indicated that the mitochondrial protein-coding genes (PCGs) of Galloanseres species have predominantly undergone purifying selection. The free-ratio model revealed that the evolutionary rates of COX1 and COX3 were lower than those of the other PCGs, whereas ND2 and ND6 had faster evolutionary rates. The CmC model also indicated that most PCGs in Anseriformes exhibited stronger selective constraints. Our study suggests that the distinct evolutionary trends and energy requirements of Galliformes and Anseriformes drive different evolutionary patterns in the mitogenome.
Corkscrew Technique for Extraction of Premolars and Molars in Standing Sedated Horses: Cadaveric Study and Clinical Cases
Authors: Ferreira Joao D. , Méndez-Angulo José L.
Published: May 2024

Several tooth extraction techniques are described in equine literature, and oral extraction techniques in standing sedated horses are popular among equine practitioners. The objectives of this study were to develop the corkscrew technique for cheek tooth extraction (CSET) in equine cadaver heads and evaluate this technique in clinical cases. We hypothesized that the CSET could be performed safely to extract cheek teeth in standing sedated horses. First, the CSET was attempted and developed in eight equine cadaver heads. Second, the CSET was performed in clinical cases between 2016 and 2020, and the following information was recorded: diagnosis, affected tooth, procedure duration, intraoperative difficulties, tooth size, postoperative complications, medication, hospitalization time, and 1-year follow-up. Sixteen CSET procedures were performed in eight equine skulls with a 75% success rate. In 24 clinical cases, 25 CSET procedures were attempted to extract 22 superior and 3 inferior cheek teeth. CSET was successful in 76% of procedures. Fractures of the tooth and stripping of screw threads were the major complications that led to the failure of CSET. CSET is a viable and safe technique to extract cheek teeth in standing sedated horses. Longitudinal drilling is a must for this technique to be successful.
Development of a Bead-Based Multiplex Fluorescent Immunoassay to Detect Antibodies against Maedi-Visna Virus in Sheep
Authors: Borge Anniken Jerre , Colitti Barbara , Rosati Sergio , Nordstoga Anne B. , Gjerset Britt , Udjus Kristin , Nogarol Chiara , Chellappa Stalin , Samdal Ingunn Anita , Lybeck Kari
Published: May 2024

The Maedi-visna virus (MVV) causes a persistent infection in small ruminants, and its high genetic heterogeneity affects the performance of diagnostic tests when used in different populations. Therefore, the aim of this study was to develop a bead-based multiplex immunoassay tailored to detect antibodies against a Norwegian MVV strain. We used tissue samples from 14 PCR-positive sheep from a recent MVV outbreak in Norway to sequence the viral strain and produced recombinant antigens based on sequences from one animal. The assay included commercial TM-A and recombinant Norwegian p25, p16–25 and SU5 antigens. Cut-off values for each antigen were determined using receiver operating characteristic curves on 40 ELISA-negative and 67 ELISA-positive samples from the outbreak. The intraplate and interplate repeatability were investigated by testing a quadruplicate of five samples over three days, while the analytical sensitivity (aSe) and specificity (aSp) were measured in comparison to a commercial ELISA. The repeatability showed a coefficient of variation below 15% for most positive samples. The aSe was equal or higher for the multiplex assay than the ELISA, and the aSp of each antigen was 91.7, 93.3, 95.0 and 93.3% for p25, p16–25, SU5 and TM-A, respectively. The assay shows promising results; however, further evaluations of diagnostic characteristics are necessary before implementation in the Norwegian surveillance programme.
Correction: Geldsetzer-Mendoza, C.; Riveros, J.L. Morphophysiological Responses of the Goat Mammary Gland to Water Scarcity in Arid and Semi-Arid Environments: Are They Enough to Generate Adaptation to New Climatic Challenges? Animals 2023, 13, 3825
Authors: Geldsetzer-Mendoza Carolina , Riveros José Luis
Published: May 2024

Additional Affiliation(s) [...]
Novel ATP2A2 Gene Mutation c.118G>A Causing Keratinocyte and Cardiomyocyte Disconnection in Darier Disease
Authors: Frustaci Andrea , De Luca Alessandro , Verardo Romina , Guida Valentina , Alfarano Maria , Calvieri Camilla , Sansone Luigi , Russo Matteo Antonio , Chimenti Cristina
Published: May 2024

Darier disease (DD) is an autosomal dominant disorder due to pathogenic variants of the ATP2A2 gene that causes an isolated skin manifestation based on keratinocyte disconnection and apoptosis. Systemic manifestations of DD have not been demonstrated so far, although a high incidence of neuropsychiatric syndromes suggests an involvement of the central nervous system. We report that the pathogenic ATP2A2 gene variant c.118G>A may cause cardiac involvement in patients with DD, consisting of keratinocyte and cardiomyocyte disconnection. Their common pathologic pathway, still unreported, was documented by both skin and left ventricular endomyocardial biopsies because cardiac dilatation and dysfunction appeared several decades after skin manifestations. Keratinocyte disconnection was paralleled by cardiomyocyte separation at the lateral junction. Cardiomyocyte separation was associated with cell disarray, sarcoplasmic reticulum dilatation, and increased myocyte apoptosis. Clinically, hyperkeratotic skin papules are associated with chest pain, severe muscle exhaustion, and ventricular arrhythmias that improved following administration of aminophylline, a phosphodiesterase inhibitor enhancing SERCA2 protein phosphorylation. Cardiac pathologic changes are similar to those documented in the skin, including cardiomyocyte disconnection that promotes precordial pain and cardiac arrhythmias. Phosphodiesterase inhibitors that enhance SERCA2 protein phosphorylation may substantially attenuate the symptoms.
The Effect of Denosumab on Rotator Cuff Repair in Women Aged 60 and over with Osteoporosis: A Prospective Observational Study
Authors: Kim Ki-Tae , Lee Sanghyeon , Lee Ho-Won , Kim Shi-Hyun , Lee Yong-Beom
Published: May 2024

Background: In previous studies, denosumab, a RANKL human monoclonal antibody used in osteoporosis treatment, has shown efficacy in tendon healing after rotator cuff repair. This prospective study investigated the effects of denosumab on tendon healing, re-tear rates, and clinical outcomes post rotator cuff repair in women with osteoporosis. Method: This was a prospective, observational study, employing propensity score matching for the control group. From March 2018 to March 2023, female patients over the age of 60 with normal bone density undergoing arthroscopic rotator cuff repair were selected as controls through propensity score matching (PSM) and compared with female patients of the same age group with osteoporosis who were receiving denosumab treatment. The control group was matched using 1-to-2 propensity score matching. Radiological examinations and functional outcomes were assessed preoperatively and at 6 months postoperatively. Results: In the final analysis, the study comprised 34 patients in the denosumab treatment group (Group 1) and 68 patients in the control group (Group 2). The functional scores showed significant improvement at 6 months post-surgery in both groups. No significant difference in the functional scores was observed among the groups. The re-tear rate, defined according to Sugaya’s classification (types IV and V) as re-tear, was slightly higher in Group 1 at 16.7% (6 of 34) compared to Group 2 at 11.7% (8 of 68), but the difference was not statistically significant (p = 0.469). The re-tear patterns, classified according to Rhee’s classification, also showed no significant difference among the groups (Group 1: 2/4 of 6; Group 2: 4/4 of 8; p = 0.571). The occurrence of type I re-tear exhibited no significant difference between the two groups (5.9% vs. 5.9%; p = 1.000). Conclusions: The administration of denosumab following arthroscopic rotator cuff repair in women aged 60 and over with osteoporosis resulted in a re-tear rate that was similar to that observed in patients without osteoporosis. This result suggests that denosumab administration might be beneficial for rotator cuff healing, particularly in the context of osteoporosis, a known risk factor for increased retear rates. Therefore, comprehensive osteoporosis screening and treatment should be considered in conjunction with rotator cuff repair surgery in middle-aged women.
Plasma Biomarkers for Hypertension-Mediated Organ Damage Detection: A Narrative Review
Authors: Liu Xinghui , Yang Miao , Lip Gregory Y. H. , McDowell Garry
Published: May 2024

Hypertension (HT) is a disease that poses a serious threat to human health, mediating organ damage such as the cardiovascular (CV) system, kidneys, central nervous system (CNS), and retinae, ultimately increasing the risk of death due to damage to the entire vascular system. Thus, the widespread prevalence of hypertension brings enormous health problems and socioeconomic burdens worldwide. The goal of hypertension management is to prevent the risk of hypertension-mediated organ damage and excess mortality of cardiovascular diseases. To achieve this goal, hypertension guidelines recommend accurate monitoring of blood pressure and assessment of associated target organ damage. Early identification of organ damage mediated by hypertension is therefore crucial. Plasma biomarkers as a non-invasive test can help identify patients with organ damage mediated by hypertension who will benefit from antihypertensive treatment optimization and improved prognosis. In this review, we provide an overview of some currently available, under-researched, potential plasma biomarkers of organ damage mediated by hypertension, looking for biomarkers that can be detected by simple testing to identify hypertensive patients with organ damage, which is of great significance in clinical work. Natriuretic peptides (NPs) can be utilized as a traditional biomarker to detect hypertension-mediated organ damage, especially for heart failure. Nevertheless, we additionally may need to combine two or more plasma biomarkers to monitor organ damage in the early stages of hypertension.
The Pathway-Selective Dependence of Nitric Oxide for Long-Term Potentiation in the Anterior Cingulate Cortex of Adult Mice
Authors: Chen Qi-Yu , Wan Jinjin , Ma Yujie , Zhuo Min
Published: May 2024

Nitric oxide (NO) is a key diffusible messenger in the mammalian brain. It has been proposed that NO may diffuse in retrograde into presynaptic terminals, contributing to the induction of hippocampal long-term potentiation (LTP). Here, we present novel evidence that NO is selectively required for the synaptic potentiation of the interhemispheric projection in the anterior cingulate cortex (ACC). Unilateral low-frequency stimulation (LFS) induced a short-term synaptic potentiation on the contralateral ACC through the corpus callosum (CC). The use of the antagonists of the NMDA receptor (NMDAR), or the inhibitor of the L-type voltage-dependent Ca2+ channels (L-VDCCs), blocked the induction of this ACC-ACC potentiation. In addition, the inhibitor of NO synthase, or inhibitors for its downstream signaling pathway, also blocked this ACC-ACC potentiation. However, the application of the NOS inhibitor blocked neither the local electric stimulation-induced LTP nor the stimulation-induced recruitment of silent responses. Our results present strong evidence for the pathway-selective roles of NO in the LTP of the ACC.
Insights into Calpain Activation and Rho-ROCK Signaling in Parkinson’s Disease and Aging
Authors: Gathings Amy , Zaman Vandana , Banik Narendra L. , Haque Azizul
Published: May 2024

Parkinson’s disease (PD), a progressive neurodegenerative disease, has no cure, and current therapies are not effective at halting disease progression. The disease affects mid-brain dopaminergic neurons and, subsequently, the spinal cord, contributing to many debilitating symptoms associated with PD. The GTP-binding protein, Rho, plays a significant role in the cellular pathology of PD. The downstream effector of Rho, Rho-associated kinase (ROCK), plays multiple functions, including microglial activation and induction of inflammatory responses. Activated microglia have been implicated in the pathology of many neurodegenerative diseases, including PD, that initiate inflammatory responses, leading to neuron death. Calpain expression and activity is increased following glial activation, which triggers the Rho-ROCK pathway and induces inflammatory T cell activation and migration as well as mediates toxic α-synuclein (α-syn) aggregation and neuron death, indicating a pivotal role for calpain in the inflammatory and degenerative processes in PD. Increased calpain activity and Rho-ROCK activation may represent a new mechanism for increased oxidative damage in aging. This review will summarize calpain activation and the role of the Rho-ROCK pathway in oxidative stress and α-syn aggregation, their influence on the neurodegenerative process in PD and aging, and possible strategies and research directions for therapeutic intervention.
Benzamide Trimethoprim Derivatives as Human Dihydrofolate Reductase Inhibitors—Molecular Modeling and In Vitro Activity Study
Authors: Drozdowska Danuta , Wróbel-Tałałaj Agnieszka , Parzych Cezary , Ratkiewicz Artur
Published: May 2024

Human dihydrofolate reductase (hDHFR) is an essential cellular enzyme, and inhibiting its activity is a promising strategy for cancer therapy. We have chosen the trimethoprim molecule (TMP) as a model compound in our search for a new class of hDHFR inhibitors. We incorporated an amide bond, a structural element typical of netropsin, a ligand that binds selectively in the minor groove of DNA, into the molecules of TMP analogs. In this work, we present previously obtained and evaluated eleven benzamides (JW1–JW8; MB1, MB3, MB4). Recently, these compounds were specifically projected as potential inhibitors of the enzymes acetylcholinesterase (AChE) and β-secretase (BACE1). JW8 was most active against AChE, with an inhibitory concentration of AChE IC50 = 0.056 µM, while the IC50 for donepezil was 0.046 µM. This compound was also the most active against the BACE1 enzyme. The IC50 value was 9.01 µM compared to that for quercetin, with IC50 = 4.89 µM. All the benzamides were active against hDHFR, with IC50 values ranging from 4.72 to 20.17 µM, and showed activity greater than TMP (55.26 µM). Quantitative results identified the derivatives JW2 and JW8 as the most promising. A molecular modeling study demonstrates that JW2 interacts strongly with the key residue Gly-117, while JW8 interacts strongly with Asn-64 and Arg-70. Furthermore, JW2 and JW8 demonstrate the ability to stabilize the hDHFR enzyme, despite forming fewer hydrogen bonds with the protein compared to reference ligands. It can be concluded that this class of compounds certainly holds great promise for good active leads in medicinal chemistry.
Dysregulated Coagulation and Fibrinolysis Are Present in Patients Admitted to the Emergency Department with Acute Hypoxemic Respiratory Failure: A Prospective Study
Authors: Keskinidou Chrysi , Vassiliou Alice Georgia , Papoutsi Elena , Jahaj Edison , Dimopoulou Ioanna , Siempos Ilias , Kotanidou Anastasia
Published: May 2024

Acute hypoxemic respiratory failure (AHRF) is defined as acute and progressive, and patients are at a greater risk of developing acute respiratory distress syndrome (ARDS). Until now, most studies have focused on prognostic and diagnostic biomarkers in ARDS. Since there is evidence supporting a connection between dysregulated coagulant and fibrinolytic pathways in ARDS progression, it is plausible that this dysregulation also exists in AHRF. The aim of this study was to explore whether levels of soluble endothelial protein C receptor (sEPCR) and plasminogen differentiate patients admitted to the emergency department (ED) with AHRF. sEPCR and plasminogen levels were measured in 130 AHRF patients upon ED presentation by ELISA. Our results demonstrated that patients presenting to the ED with AHRF had elevated levels of sEPCR and plasminogen. It seems that dysregulation of coagulation and fibrinolysis occur in the early stages of respiratory failure requiring hospitalisation. Further research is needed to fully comprehend the contribution of sEPCR and plasminogen in AHRF.
Selenium and Arsenic Levels, Prevalence of Common Variants of Genes Involved in Their Metabolism, and Psoriasis Disease
Authors: Dębniak Tadeusz , Baszuk Piotr , Duchnik Ewa , Rowińska Karolina , Rogoża-Janiszewska Emilia , Boer Magdalena , Kiedrowicz Magdalena , Marchlewicz Mariola , Watola Daniel , Feherpataky Martyna , Derkacz Róża , Dębniak Anna , Marciniak Wojciech , Gołębiewska Katarzyna , Lubiński Jan , Scott Rodney J. , Gronwald Jacek
Published: May 2024

Using an Inductively Coupled Plasma Mass Spectrometer we measured the concentration of selenium and arsenic in serum and blood samples from 336 unselected psoriatic patients and 336 matched healthy controls to evaluate any associations with the clinical course of the disease. We genotyped 336 patients and 903 matched controls to evaluate the prevalence of SOD2 (rs4880), CAT (rs1001179), GPX1 (rs1050450), and DMGDH (rs921943) polymorphisms using Taqman assays. The mean selenium (Se) level in serum was 74 µg/L in patients and 86 µg/L in controls (p < 0.001). The mean Se level in blood was 95 µg/L in patients and 111 µg/L in controls (p < 0.001). Psoriasis risk was greatest among participants with the lowest serum (<68.75 µg/L, OR: 8.30; p < 0.001) and lowest blood concentrations of Se (<88.04 µg/L, OR: 10.3; p < 0.001). Similar results were observed in subgroups of males and females. We found an inverse correlation of selenium levels with PASI, NAPSI, and BSA scores. There was no significant difference in the distribution of the CAT, GPX1, DMGDH, and SOD2 polymorphisms. Among carriers of rs4880, rs1001179, and rs921943 polymorphisms, blood selenium levels were significantly lower. The mean arsenic level in serum was 0.79 µg/L in patients and 0.7 µg/L in controls (p = 0.2). The mean concentration in blood was 1.1 µg/L in patients and 1.3 µg/L in controls (p < 0.001). In conclusion, we found that lower selenium levels, in blood and serum, are associated with psoriasis risk and its more severe course. Future prospective studies should focus on the optimalisation of the concentration of this trace element not only for prophylactic guidance but also to support the treatment of this disease.
Can Plants Perceive Human Gestures? Using AI to Track Eurythmic Human–Plant Interaction
Authors: Gil Alvaro Francisco , Weinbeer Moritz , Gloor Peter A.
Published: May 2024

This paper explores if plants are capable of responding to human movement by changes in their electrical signals. Toward that goal, we conducted a series of experiments, where humans over a period of 6 months were performing different types of eurythmic gestures in the proximity of garden plants, namely salad, basil, and tomatoes. To measure plant perception, we used the plant SpikerBox, which is a device that measures changes in the voltage differentials of plants between roots and leaves. Using machine learning, we found that the voltage differentials over time of the plant predict if (a) eurythmy has been performed, and (b) which kind of eurythmy gestures has been performed. We also find that the signals are different based on the species of the plant. In other words, the perception of a salad, tomato, or basil might differ just as perception of different species of animals differ. This opens new ways of studying plant ecosystems while also paving the way to use plants as biosensors for analyzing human movement.
Multi-Strategy Improved Dung Beetle Optimization Algorithm and Its Applications
Authors: Ye  Mingjun , Zhou Heng , Yang Haoyu , Hu Bin , Wang Xiong
Published: May 2024

The dung beetle optimization (DBO) algorithm, a swarm intelligence-based metaheuristic, is renowned for its robust optimization capability and fast convergence speed. However, it also suffers from low population diversity, susceptibility to local optima solutions, and unsatisfactory convergence speed when facing complex optimization problems. In response, this paper proposes the multi-strategy improved dung beetle optimization algorithm (MDBO). The core improvements include using Latin hypercube sampling for better population initialization and the introduction of a novel differential variation strategy, termed “Mean Differential Variation”, to enhance the algorithm’s ability to evade local optima. Moreover, a strategy combining lens imaging reverse learning and dimension-by-dimension optimization was proposed and applied to the current optimal solution. Through comprehensive performance testing on standard benchmark functions from CEC2017 and CEC2020, MDBO demonstrates superior performance in terms of optimization accuracy, stability, and convergence speed compared with other classical metaheuristic optimization algorithms. Additionally, the efficacy of MDBO in addressing complex real-world engineering problems is validated through three representative engineering application scenarios namely extension/compression spring design problems, reducer design problems, and welded beam design problems.