bluuebunny/crossref_metadata_2025 huggingface dataset:
Task Scheduling for Federated Learning in Edge Cloud Computing Environments by Using Adaptive-Greedy Dingo Optimization Algorithm and Binary Salp Swarm Algorithm
Authors: Cai Weihong , Duan Fengxi
Published: October 2023

With the development of computationally intensive applications, the demand for edge cloud computing systems has increased, creating significant challenges for edge cloud computing networks. In this paper, we consider a simple three-tier computational model for multiuser mobile edge computing (MEC) and introduce two major problems of task scheduling for federated learning in MEC environments: (1) the transmission power allocation (PA) problem, and (2) the dual decision-making problems of joint request offloading and computational resource scheduling (JRORS). At the same time, we factor in server pricing and task completion, in order to improve the user-friendliness and fairness in scheduling decisions. The solving of these problems simultaneously ensures both scheduling efficiency and system quality of service (QoS), to achieve a balance between efficiency and user satisfaction. Then, we propose an adaptive greedy dingo optimization algorithm (AGDOA) based on greedy policies and parameter adaptation to solve the PA problem and construct a binary salp swarm algorithm (BSSA) that introduces binary coding to solve the discrete JRORS problem. Finally, simulations were conducted to verify the better performance compared to the traditional algorithms. The proposed algorithm improved the convergence speed of the algorithm in terms of scheduling efficiency, improved the system response rate, and found solutions with a lower energy consumption. In addition, the search results had a higher fairness and system welfare in terms of system quality of service.
Electromagnetic, Air and Fat Frying of Plant Protein-Based Batter-Coated Foods
Authors: Bhuiyan Md. Hafizur Rahman , Ngadi Michael O.
Published: October 2023

There is growing consumer and food industry interest in plant protein-based foods. However, quality evolution of plant protein-based meat analog (MA) is still a rarely studied subject. In this study, wheat and rice flour-based batter systems were used to coat plant protein-based MA, and were partially fried (at 180 °C, 1 min) in canola oil, subsequently frozen (at −18 °C) and stored for 7 days. Microwave heating (MH), infrared heating (IH), air frying (AF) and deep-fat frying (DFF) processes were employed on parfried frozen MA products, and their quality evolution was investigated. Results revealed that the fat content of MH-, IH- and AF-treated products was significantly (p < 0.05) lower than DFF-treated counterparts. Batter coatings reduced fat uptake in DFF of MA-based products. Both the batter formulations and cooking methods impacted the process parameters and quality attributes (cooking loss, moisture, texture, color) of MA-based coated food products. Moreover, the post-cooking stability of moisture and textural attributes of batter-coated MA-based products was impacted by both the batter formulations and cooking methods. Glass transition temperature (Tg) of MA-based products’ crust ranged from −20.0 °C to −23.1 °C, as determined with differential scanning calorimetry. ATR-FTIR spectroscopy and scanning electron microscopy analysis revealed that surface structural–chemical evolution of MA-based products was impacted by both the coating formulations and cooking methods. Overall, AF has been found as a suitable substitute for DFF in terms of studied quality attributes of meat analog-based coated products.
Comparison between Two Competing Newton-Type High Convergence Order Schemes for Equations on Banach Spaces
Authors: Argyros Ioannis K. , Singh Manoj K. , Regmi Samundra
Published: October 2023

We carried out a local comparison between two ninth convergence order schemes for solving nonlinear equations, relying on first-order Fréchet derivatives. Earlier investigations require the existence as well as the boundedness of derivatives of a high order to prove the convergence of these schemes. However, these derivatives are not in the schemes. These assumptions restrict the applicability of the schemes, which may converge. Numerical results along with a boundary value problem are given to examine the theoretical results. Both schemes are symmetrical not only in the theoretical results (formation and convergence order), but the numerical and dynamical results are also similar. We calculated the convergence radii of the nonlinear schemes. Moreover, we obtained the extraneous fixed points for the proposed schemes, which are repulsive and are not part of the solution space. Lastly, the theoretical and numerical results are supported by the dynamic results, where we plotted basins of attraction for a selected test function.
A Quicker Iteration Method for Approximating the Fixed Point of Generalized α-Reich-Suzuki Nonexpansive Mappings with Applications
Authors: Ali Danish , Ali Shahbaz , Pompei-Cosmin Darab , Antoniu Turcu , Zaagan Abdullah A. , Mahnashi Ali M.
Published: October 2023

Fixed point theory is a branch of mathematics that studies solutions that remain unchanged under a given transformation or operator, and it has numerous applications in fields such as mathematics, economics, computer science, engineering, and physics. In the present article, we offer a quicker iteration technique, the D** iteration technique, for approximating fixed points in generalized α-nonexpansive mappings and nearly contracted mappings. In uniformly convex Banach spaces, we develop weak and strong convergence results for the D** iteration approach to the fixed points of generalized α-nonexpansive mappings. In order to demonstrate the effectiveness of our recommended iteration strategy, we provide comprehensive analytical, numerical, and graphical explanations. Here, we also demonstrate the stability consequences of the new iteration technique. We approximately solve a fractional Volterra–Fredholm integro-differential problem as an application of our major findings. Our findings amend and expand upon some previously published results.
Preparation and Application of Polyrotaxane Cross-Linking Agent Based on Cyclodextrin in Gel Materials Field
Authors: Liu Siyuan , Zheng Jingxi , Wang Jiaqin , Liu Shanghao , Zhang Xianli , Bao Dan , Zhang Peng
Published: October 2023

The cross-linking point of a conventional chemical cross-linking agent is fixed. Therefore, gels that are prepared with a conventional cross-linking agent have poor deformability, strength, shear resistance, and further properties. Some researchers have prepared a new cross-linking agent using cyclodextrin (CD). In a polyrotaxane cross-linking agent, the cross-linking points can slide freely along the molecule chain. The special “slide ring” structure can provide better elongation, strength, and other properties to gels, which can effectively expand the application of the gel’s materials. This paper summarizes the preparation methods and applications from different types of CD and compares the improvements of properties (swelling, viscoelastic properties, etc.). In addition, the current results of our group are presented, and some ideas are provided for the development of polyrotaxane cross-linking agents.
Genotype–Phenotype Correlations in 293 Russian Patients with Causal Fabry Disease Variants
Authors: Savostyanov Kirill , Pushkov Alexander , Zhanin Ilya , Mazanova Natalya , Pakhomov Alexander , Trufanova Elena , Alexeeva Alina , Sladkov Dmitry , Kuzenkova Ludmila , Asanov Aliy , Fisenko Andrey
Published: October 2023

Background: Fabry disease (FD) is a rare hereditary multisystem disease caused by variants of the GLA gene. Determination of GLA gene variants and identification of genotype–phenotype correlations allow us to explain the features of FD associated with predominant damage of one or another system, both in the classical and atypical forms of FD, as well as in cases with late manifestation and involvement of one of the systems. Methods: The study included 293 Russian patients with pathogenic variants of the GLA gene, which were identified as a result of various selective screening programs. Screening was carried out for 48,428 high-risk patients using a two-step diagnostic algorithm, including the determination of the concentration of the biomarker lyso-Gb3 as a first-tier test. Screening of atypical FD among patients with HCM was carried out via high-throughput sequencing in another 2427 patients. Results: 102 (0.20%) cases of FD were identified among unrelated patients as a result of the study of 50,855 patients. Molecular genetic testing allowed us to reveal the spectrum and frequencies of 104 different pathogenic variants of the GLA gene in 293 examined patients from 133 families. The spectrum and frequencies of clinical manifestations in patients with FD, including 20 pediatric patients, were described. Correlations between the concentration of the lyso-Gb3 biomarker and the type of pathogenic variants of the GLA gene have been established. Variants identified in patients with early stroke were described, and the association of certain variants with the development of stroke was established. Conclusions: The results of a large-scale selective FD screening, as well as clinical and molecular genetic features, in a cohort of 293 Russian patients with FD are described.
Subfunctionalization of Parental Polyamine Oxidase (PAO) Genes in the Allopolyploid Tobacco Nicotiana tabacum (L.)
Authors: Benkő Péter , Kaszler Nikolett , Gémes Katalin , Fehér Attila
Published: October 2023

Polyamines play an important role in developmental and environmental stress responses in plants. Polyamine oxidases (PAOs) are flavin-adenine-dinucleotide-dependent enzymes associated with polyamine catabolism. In this study, 14 genes were identified in the tobacco genome that code for PAO proteins being named based on their sequence homology with Arabidopsis PAOs (AtPAO1-5): NtPAO1A-B; NtPAO2A-C, NtPAO4A-D, and NtPAO5A-E. Sequence analysis confirmed that the PAO gene family of the allopolyploid hybrid Nicotiana tabacum is not an exact combination of the PAO genes of the maternal Nicotiana sylvestris and paternal Nicotiana tomentosiformis ones. The loss of the N. sylvestris homeolog of NtPAO5E and the gain of an extra NtPAO2 copy, likely of Nicotiana othophora origin, was revealed. The latter adds to the few pieces of evidence suggesting that the paternal parent of N. tabacum was an introgressed hybrid of N. tomentosiformis and N. othophora. Gene expression analysis indicated that all 14 PAO genes kept their expression following the formation of the hybrid species. The homeologous gene pairs showed similar or opposite regulation depending on the investigated organ, applied stress, or hormone treatment. The data indicate that the expression pattern of the homeologous genes is diversifying in a process of subfunctionalization.
Numerical Modelling and Investigation of the Impact Behaviour of Single Guardrail Posts
Authors: Soliman Mohamed , Cudmani Roberto
Published: October 2023

Vehicle restraint systems are vital hardware elements in road safety engineering. The certification process of a vehicle restraint system includes full-scale crash tests, component testing and numerical simulation of these tests. To achieve reliable crash test simulation results, the soil–post interaction must be modelled to capture the behaviour realistically. There is no standardised approach for modelling the soil–post interaction in the praxis. In this study, the finite element method is utilised to investigate the soil–post response under quasi-static and dynamic impact loading. Two different modelling techniques are applied for this purpose. The first technique is the finite element continuum method, with the soil modelled using the advanced hypoplastic constitutive relation and calibrated using laboratory test data. The second technique is a lumped-parameter model, for which a systematic parameters calibration routine using basic soil properties is introduced. The numerical models are validated using a series of full-scale field tests performed by the authors on single posts in standard road shoulder materials. The performance comparison of the investigated modelling techniques shows that the hypoplastic constitutive relation can capture the post behaviour realistically under different loading conditions using the same parameter set. The introduced lumped-parameter model adequately simulates the post behaviour with high computational efficiency, which is very important when simulating several posts. The conducted parametric study elucidates that the soil’s relative density, the post’s embedment length, and the post-section modulus govern the single post’s lateral load-bearing behaviour and energy dissipation capacity.
The Global Metabolome Profiles of Four Varieties of Lonicera caerulea, Established via Tandem Mass Spectrometry
Authors: Razgonova Mayya P. , Navaz Muhammad Amjad , Sabitov Andrey S. , Zinchenko Yulia N. , Rusakova Elena A. , Petrusha Elena N. , Golokhvast Kirill S. , Tikhonova Nadezhda G.
Published: October 2023

Blue honeysuckle (Lonicera caerulea L.) bears dietary fruits that are rich in bioactive compounds. However, information on the metabolome profiles of honeysuckle varieties grown in Russia is limited. In this study, we employed tandem mass spectrometry to study the metabolome profiles of four L. caerulea varieties (Volhova, Tomichka, Goluboe vereteno, and Amfora) grown in two geographical locations in Russia, i.e., the Russian Far East and St. Petersburg. We observed that the metabolome profiles of the four varieties grown in two locations differ significantly, particularly in the polyphenol’s other compound classes. We were able to identify 122 bioactive compounds in extracts from honeysuckle berries, 75 compounds from the polyphenol group and 47 compounds from other chemical groups. Thirty chemical constituents from the polyphenol group (flavones jaceosidin, cirsiliol, sophoraisoflavone A, chrysoeriol-O-hexoside, flavonols dimethylquercetin-3-O-dehexoside, rhamnocitrin, rhamnetin II, stilbenes pinosylvin, resveratrol, dihydroresveratrol, etc.) and twenty-seven from other chemical groups were identified. The largest number of unique polyphenols is characteristic of the variety Tomichka, the selection of the regional state unitary enterprise “Bakcharskoye”, from the free pollination of L. caerulea, originating in the Primorsky Territory of Russia (L. caerulea subspecies Turczaninow). This genotype has the highest number of similar unique polyphenols, regardless of where it was grown. Blue honeysuckle genotypes originating from Primorsky Krai in Russia can be used in various breeding programs in order to improve and enrich the biochemical composition of fruits. It should also be noted that, regardless of the place of cultivation, the total amount of unique polyphenols remains quite large. Attention should be paid to the Volhova honeysuckle variety, obtained through gamma irradiation of the Pavlovskaya variety (Kamchatka ecotype). This sample is characterized by a stable composition of biologically active substances, regardless of the growing area. These data could support future research on the production of a variety of pharmaceutical products containing ultrapure extracts of L. caerulea.
The Effect of Mental Activation of One’s Pet Dog on Stress Reactivity
Authors: Rodriguez Kerri E. , Graham Dan J. , Lucas-Thompson Rachel G.
Published: October 2023

Research suggests that mental activation of human social support may reduce stress reactivity. However, the extent to which social support from pets elicits a similar effect has been less explored. This study aims to determine whether the mental activation of one’s pet dog reduces stress reactivity to a subsequent experimental stressor. In a 2 × 2 design, 132 dog-owning participants (Mage = 20.14; 80% female) were randomly assigned to one of two mental activation conditions (pet dog; general) and one of two stressor conditions (social-evaluative; cognitive). Data were analyzed with two-way ANOVAs with self-reported (positive/negative affect, negative self-evaluation) and physiological (blood pressure, heart rate) dependent variables. Results indicated that participants randomized to the pet dog mental activation condition had smaller decreases in positive affect from baseline to post-stressor compared to the general mental activation condition. However, there were no significant interactions between time and mental activation condition on negative affect, negative self-evaluation, heart rate, or blood pressure. Thus, the mental activation of one’s pet dog had a minimal effect on stress reactivity to a cognitive or social-evaluative stressor. Results suggest that the physical presence of an animal may be an essential mechanism underlying the benefits of animal-derived social support.
Mental Health of Immigrant Children and Adolescents (6–17 Years) in Canada: Evidence from the Canadian Health Measures Survey
Authors: Salami Oluwabukola Oladunni , Yaskina Maryna , Georgiades Katholiki , Diaz Esperanza , Hegadoren Kathleen , Meherali Salima , Yohani Sophie , Senthilselvan Ambikaipakan
Published: October 2023

Background: Studies indicate a higher prevalence of mental health problems among immigrants, but findings on immigrant children and adolescents are mixed. We sought to understand the magnitude of differences in mental health indicators between immigrant and non-immigrant children and adolescents in Canada and the influence of age, sex, household income, and household education. Methods: We completed a secondary analysis of data from the Canadian Health Measures Survey, using a pooled estimate method to combine data from four survey cycles. A weighted logistic regression was used to estimate the unadjusted and adjusted odds ratios with 95% confidence intervals. Results: We found an association between the mental health of immigrant versus non-immigrant children and adolescents (6–17 years) as it relates to emotional problems and hyperactivity. Immigrant children and adolescents had better outcomes with respect to emotional problems and hyperactivity/inattention compared to non-immigrant children and adolescents. Lower household socioeconomic status was associated with poorer mental health in children and adolescents. Conclusion: No significant differences in overall mental health status were evident between immigrant and non-immigrant children and adolescents in Canada but differences exist in emotional problems and hyperactivity. Sex has an influence on immigrant child mental health that varies depending on the specific mental health indicator.
Nothing in Excess: Physical Activity, Health, and Life World in Senegalese Fulani Male Pastoralists, a Mixed Method Approach
Authors: Chevé Dominique , Macia Enguerran , Diallo Moussa , Lalys Loic , Diallo Amadou Hamath , Sow Sidaty , Bergouignan Audrey , Duboz Priscilla
Published: October 2023

Objectives: The goal of this study was to evaluate the application of the short form of the International Physical Activity Questionnaire Survey (IPAQ-SF) in the rural Senegalese Fulani pastoralist population by combining quantitative and qualitative methods. Design and participants: For the quantitative method, 101 men completed the IPAQ-SF questionnaire measuring moderate, vigorous, and walking physical activity. Self-rated health, BMI, and sociodemographic variables were also collected. With regard to the qualitative methods, a total of 22 participants were recruited and interviewed. Four themes were addressed, including (i) physical activity (PA) and its definition, description, related experiences, and representations of social actors; (ii) PA and health; (iii) PA and sport; and (iv) the body and Fulani world of life (i.e., Pulaagu/Ndimaagu). Results: Sahelian herders have a high level of self-reported PA and a low amount of daily sitting time. The measure of PA as proposed by the IPAQ-SF is not adapted to the Senegalese Ferlo pastoralists, mainly because this scale gives too much importance to leisure-time PA, perceived as unproductive energy expenditure, which is factually and symbolically antinomic to the Fulani lifeworld. Thus, neither intense nor moderate PA is related to self-rated health. However, sedentary lifestyles are linked to self-rated health and, therefore, to mortality and morbidity in Fulani pastoralists. Finally, walking, which is the dominant PA during transhumance and herd surveillance, is related to BMI. It therefore represents a protective factor against the occurrence of overweight and associated chronic non-communicable diseases. Conclusion: The mixed method approach developed in this study has shown that the IPAQ-SF is not a valid measure of PA in the population of Fulani male herders from the Ferlo region, given that unproductive energy expenditure is incompatible with the Fulani way of life, which condemns excess and immoderation.
Amyloid Fibrils Produced by Streptococcus sanguinis Contribute to Biofilm Formation and Immune Evasion
Authors: Franco Eduardo M. , Alves Lívia A. , Naveed Hassan , Freitas Victor A. A. , Bastos Débora C. , Mattos-Graner Renata O.
Published: October 2023

Bacterial surface proteins assembled into amyloids contribute to biofilm formation and host immune evasion. Streptococcus sanguinis, a pioneer colonizer of teeth commonly involved in cardiovascular infections, expresses about thirty-three proteins anchored to the cell wall by sortase A. Here, we characterized the production of amyloid in S. sanguinis strains differing in biofilm and immune evasion phenotypes and investigated the role of sortase A in amyloidogenesis. Amyloid was identified in biofilms formed by nine strains, using Congo red (CR) staining and cross-polarized light microscopy. Additionally, EGCG, an amyloid inhibitor, impaired biofilm maturation in a strain-specific fashion. The amounts of amyloid-like components quantified in culture fluids of nine strains using thioflavin T and fluorimetry negatively correlated with bacterial binding to complement-activating proteins (SAP, C1q), C3b deposition and rates of opsonophagocytosis in PMNs, implying amyloid production in immune evasion. The deletion of the sortase A gene (srtA) in strain SK36 compromised amyloid production and sucrose-independent biofilm maturation. The srtA mutant further showed increased susceptibility to C3b deposition and altered interactions with PMNs as well as reduced persistence in human blood. These findings highlight the contribution of amyloids to biofilm formation and host immune evasion in S. sanguinis strains, further indicating the participation of sortase A substrates in amyloidogenesis.
CAR-T Cell Therapy: From the Shop to Cancer Therapy
Authors: Uscanga-Palomeque Ashanti Concepción , Chávez-Escamilla Ana Karina , Alvizo-Báez Cynthia Aracely , Saavedra-Alonso Santiago , Terrazas-Armendáriz Luis Daniel , Tamez-Guerra Reyes S. , Rodríguez-Padilla Cristina , Alcocer-González Juan Manuel
Published: October 2023

Cancer is a worldwide health problem. Nevertheless, new technologies in the immunotherapy field have emerged. Chimeric antigen receptor (CAR) technology is a novel biological form to treat cancer; CAR-T cell genetic engineering has positively revolutionized cancer immunotherapy. In this paper, we review the latest developments in CAR-T in cancer treatment. We present the structure of the different generations and variants of CAR-T cells including TRUCK (T cells redirected for universal cytokine killing. We explain the approaches of the CAR-T cells manufactured ex vivo and in vivo. Moreover, we describe the limitations and areas of opportunity for this immunotherapy and the current challenges of treating hematological and solid cancer using CAR-T technology as well as its constraints and engineering approaches. We summarize other immune cells that have been using CAR technology, such as natural killer (NK), macrophages (M), and dendritic cells (DC). We conclude that CAR-T cells have the potential to treat not only cancer but other chronic diseases.
GroEL-Proteotyping of Bacterial Communities Using Tandem Mass Spectrometry
Authors: Klaes Simon , Madan Shobhit , Deobald Darja , Cooper Myriel , Adrian Lorenz
Published: October 2023

Profiling bacterial populations in mixed communities is a common task in microbiology. Sequencing of 16S small subunit ribosomal-RNA (16S rRNA) gene amplicons is a widely accepted and functional approach but relies on amplification primers and cannot quantify isotope incorporation. Tandem mass spectrometry proteotyping is an effective alternative for taxonomically profiling microorganisms. We suggest that targeted proteotyping approaches can complement traditional population analyses. Therefore, we describe an approach to assess bacterial community compositions at the family level using the taxonomic marker protein GroEL, which is ubiquitously found in bacteria, except a few obligate intracellular species. We refer to our method as GroEL-proteotyping. GroEL-proteotyping is based on high-resolution tandem mass spectrometry of GroEL peptides and identification of GroEL-derived taxa via a Galaxy workflow and a subsequent Python-based analysis script. Its advantage is that it can be performed with a curated and extendable sample-independent database and that GroEL can be pre-separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to reduce sample complexity, improving GroEL identification while simultaneously decreasing the instrument time. GroEL-proteotyping was validated by employing it on a comprehensive raw dataset obtained through a metaproteome approach from synthetic microbial communities as well as real human gut samples. Our data show that GroEL-proteotyping enables fast and straightforward profiling of highly abundant taxa in bacterial communities at reasonable taxonomic resolution.
Review of Eukaryote Cellular Membrane Lipid Composition, with Special Attention to the Fatty Acids
Authors: Ali Omeralfaroug , Szabó András
Published: October 2023

Biological membranes, primarily composed of lipids, envelop each living cell. The intricate composition and organization of membrane lipids, including the variety of fatty acids they encompass, serve a dynamic role in sustaining cellular structural integrity and functionality. Typically, modifications in lipid composition coincide with consequential alterations in universally significant signaling pathways. Exploring the various fatty acids, which serve as the foundational building blocks of membrane lipids, provides crucial insights into the underlying mechanisms governing a myriad of cellular processes, such as membrane fluidity, protein trafficking, signal transduction, intercellular communication, and the etiology of certain metabolic disorders. Furthermore, comprehending how alterations in the lipid composition, especially concerning the fatty acid profile, either contribute to or prevent the onset of pathological conditions stands as a compelling area of research. Hence, this review aims to meticulously introduce the intricacies of membrane lipids and their constituent fatty acids in a healthy organism, thereby illuminating their remarkable diversity and profound influence on cellular function. Furthermore, this review aspires to highlight some potential therapeutic targets for various pathological conditions that may be ameliorated through dietary fatty acid supplements. The initial section of this review expounds on the eukaryotic biomembranes and their complex lipids. Subsequent sections provide insights into the synthesis, membrane incorporation, and distribution of fatty acids across various fractions of membrane lipids. The last section highlights the functional significance of membrane-associated fatty acids and their innate capacity to shape the various cellular physiological responses.
Cistanche deserticola Polysaccharide Reduces Inflammation and Aging Phenotypes in the Dermal Fibroblasts through the Activation of the NRF2/HO-1 Pathway
Authors: Takaya Kento , Asou Toru , Kishi Kazuo
Published: October 2023

Dermal fibroblasts maintain the skin homeostasis by interacting with the epidermis and extracellular matrix. Their senescence contributes to functional defects in the skin related to aging. Therefore, there is an urgent need for novel therapeutic agents that could inhibit fibroblast senescence. In this study, we investigated the effects of Cistanche deserticola polysaccharide (CDP), a natural anti-inflammatory component, on the progression of senescence in human dermal fibroblasts. Normal human dermal fibroblasts (NHDFs) were cultured in passages, and highly senescent cells were selected as senescent cells. CDP treatment increased the cell proliferation in senescent NHDFs and decreased the proportion of senescence-associated-β-galactosidase-positive cells. The treatment suppressed the senescence-related secretory phenotype, and reactive oxygen species (ROS) production was reduced, alleviating H2O2-induced oxidative stress. CDP mitigated ROS formation via the nuclear factor erythroid 2-related factor/heme oxygenase-1 pathway in senescent cells and was involved in the suppression of upstream p-extracellular signal-regulated kinase. These results indicate that CDP is an antioxidant that can alleviate age-related inflammation and may be a useful compound for skin anti-aging.
Monoclonal Antibodies as SARS-CoV-2 Serology Standards: Experimental Validation and Broader Implications for Correlates of Protection
Authors: Wang Lili , Patrone Paul N. , Kearsley Anthony J. , Izac Jerilyn R. , Gaigalas Adolfas K. , Prostko John C. , Kwon Hyung Joon , Tang Weichun , Kosikova Martina , Xie Hang , Tian Linhua , Elsheikh Elzafir B. , Kwee Edward J. , Kemp Troy , Jochum Simon , Thornburg Natalie , McDonald L. Clifford , Gundlapalli Adi V. , Lin-Gibson Sheng
Published: October 2023

COVID-19 has highlighted challenges in the measurement quality and comparability of serological binding and neutralization assays. Due to many different assay formats and reagents, these measurements are known to be highly variable with large uncertainties. The development of the WHO international standard (WHO IS) and other pool standards have facilitated assay comparability through normalization to a common material but does not provide assay harmonization nor uncertainty quantification. In this paper, we present the results from an interlaboratory study that led to the development of (1) a novel hierarchy of data analyses based on the thermodynamics of antibody binding and (2) a modeling framework that quantifies the probability of neutralization potential for a given binding measurement. Importantly, we introduced a precise, mathematical definition of harmonization that separates the sources of quantitative uncertainties, some of which can be corrected to enable, for the first time, assay comparability. Both the theory and experimental data confirmed that mAbs and WHO IS performed identically as a primary standard for establishing traceability and bridging across different assay platforms. The metrological anchoring of complex serological binding and neuralization assays and fast turn-around production of an mAb reference control can enable the unprecedented comparability and traceability of serological binding assay results for new variants of SARS-CoV-2 and immune responses to other viruses.
From the Skin to Distant Sites: T Cells in Psoriatic Disease
Authors: Reali Eva , Ferrari Davide
Published: October 2023

Human skin has long been known as a protective organ, acting as a mechanical barrier towards the external environment. More recent is the acquisition that in addition to this fundamental role, the complex architecture of the skin hosts a variety of immune and non-immune cells playing preeminent roles in immunological processes aimed at blocking infections, tumor progression and migration, and elimination of xenobiotics. On the other hand, dysregulated or excessive immunological response into the skin leads to autoimmune reactions culminating in a variety of skin pathological manifestations. Among them is psoriasis, a multifactorial, immune-mediated disease with a strong genetic basis. Psoriasis affects 2–3% of the population; it is associated with cardiovascular comorbidities, and in up to 30% of the cases, with psoriatic arthritis. The pathogenesis of psoriasis is due to the complex interplay between the genetic background of the patient, environmental factors, and both innate and adaptive responses. Moreover, an autoimmune component and the comprehension of the mechanisms linking chronic skin inflammation with systemic and joint manifestations in psoriatic patients is still a major challenge. The understanding of these mechanisms may offer a valuable chance to find targetable molecules to treat the disease and prevent its progression to severe systemic conditions.
Extracellular Vesicles: Emergent and Multiple Sources in Wound Healing Treatment
Authors: Sarcinella Alessandro , Femminò Saveria , Brizzi Maria Felice
Published: October 2023

Non-healing wound- and tissue-injury are commonly experienced worldwide by the aging population. The persistence of disease commonly leads to tissue infection, resulting in severe clinical complications. In the last decade, extracellular vesicles (EVs) have been considered promising and emergent therapeutic tools to improve the healing processes. Therefore, efforts have been directed to develop a cell-free therapeutic platform based on EV administration to orchestrate tissue repair. EVs derived from different cell types, including fibroblast, epithelial, and immune cells are recruited to the injured sites and in turn take part in scar formation. EVs are nano-sized particles containing a heterogeneous cargo consisting of lipids, proteins, and nucleic acids protected from degradation by their lipid bilayer. Noteworthy, since EVs have natural biocompatibility and low immunogenicity, they represent the ideal therapeutic candidates for regenerative purposes. Indeed, EVs are released by several cell types, and even if they possess unique biological properties, their functional capability can be further improved by engineering their content and functionalizing their surface, allowing a specific cell cargo delivery. Herein, we provide an overview of preclinical data supporting the contribution of EVs in the repair and regenerative processes, focusing on different naïve EV sources, as well as on their engineering, to offer a scalable and low-cost therapeutic option for tissue repair.
Effectiveness of Cerium Oxide Nanoparticles in Non-Alcoholic Fatty Liver Disease Evolution Using In Vivo and In Vitro Studies: A Systematic Review
Authors: Sandoval Cristian , Reyes Carolina , Rosas Pamela , Godoy Karina , Souza-Mello Vanessa , Farías Jorge
Published: October 2023

Nonalcoholic fatty liver disease (NAFLD) describes a spectrum of liver abnormalities, from benign steatosis to nonalcoholic steatohepatitis (NASH). Because of their antioxidant capabilities, CeNPs have sparked a lot of interest in biological applications. This review evaluated the effectiveness of CeNPs in NAFLD evolution through in vivo and in vitro studies. Databases such as MEDLINE, EMBASE, Scopus, and Web of Science were looked for studies published between 2012 and June 2023. Quality was evaluated using PRISMA guidelines. We looked at a total of nine primary studies in English carried out using healthy participants or HepG2 or LX2 cells. Quantitative data such as blood chemical markers, lipid peroxidation, and oxidative status were obtained from the studies. Our findings indicate that NPs are a possible option to make medications safer and more effective. In fact, CeNPs have been demonstrated to decrease total saturated fatty acids and foam cell production (steatosis), reactive oxygen species production and TNF-α (necrosis), and vacuolization in hepatic tissue when used to treat NAFLD. Thus, CeNP treatment may be considered promising for liver illnesses. However, limitations such as the variation in durations between studies and the utilization of diverse models to elucidate the etiology of NAFLD must be considered. Future studies must include standardized NAFLD models.
Ahf-Caltide, a Novel Polypeptide Derived from Calpastatin, Protects against Oxidative Stress Injury by Stabilizing the Expression of CaV1.2 Calcium Channel
Authors: Xue Yingchun , Zhou Shi , Yan Ling , Li Yuelin , Xu Xingrong , Wang Xianghui , Minobe Etsuko , Kameyama Masaki , Hao Liying , Hu Huiyuan
Published: October 2023

Reperfusion after ischemia would cause massive myocardial injury, which leads to oxidative stress (OS). Calcium homeostasis imbalance plays an essential role in myocardial OS injury. CaV1.2 calcium channel mediates calcium influx into cardiomyocytes, and its activity is modulated by a region of calpastatin (CAST) domain L, CSL54-64. In this study, the effect of Ahf-caltide, derived from CSL54-64, on myocardial OS injury was investigated. Ahf-caltide decreased the levels of LDH, MDA and ROS and increased heart rate, coronary flow, cell survival and SOD activity during OS. In addition, Ahf-caltide permeated into H9c2 cells and increased CaV1.2, CaVβ2 and CAST levels by inhibiting protein degradation. At different Ca2+ concentrations (25 nM, 10 μM, 1 mM), the binding of CSL to the IQ motif in the C terminus of the CaV1.2 channel was increased in a H2O2 concentration-dependent manner. CSL54-64 was predicted to be responsible for the binding of CSL to CaV1.2. In conclusion, Ahf-caltide exerted a cardioprotective effect on myocardial OS injury by stabilizing CaV1.2 protein expression. Our study, for the first time, proposed that restoring calcium homeostasis by targeting the CaV1.2 calcium channel and its regulating factor CAST could be a novel treatment for myocardial OS injury.
Oncogenic Gαq activates RhoJ through PDZ-RhoGEF
Authors: Cervantes-Villagrana Rodolfo Daniel , Color-Aparicio Víctor Manuel , Castillo-Kauil Alejandro , García-Jiménez Irving , Beltrán-Navarro Yarely Mabell , Reyes-Cruz Guadalupe , Vázquez-Prado José
Published: October 2023

Oncogenic Gαq causes uveal melanoma via non-canonical signaling pathways. This constitutively active mutant GTPase is also found in cutaneous melanoma, lung adenocarcinoma, and seminoma, as well as in benign vascular tumors, such as congenital hemangiomas. We recently described that PDZ-RhoGEF (also known as ARHGEF11), a canonical Gα12/13 effector, is enabled by Gαs Q227L to activate CdcIn addition, and we demonstrated that constitutively active Gαq interacts with the PDZ-RhoGEF DH-PH catalytic module, but does not affect its binding to RhoA or Cdc. This suggests that it guides this RhoGEF to gain affinity for other GTPases. Since RhoJ, a small GTPase of the Cdc42 subfamily, has been involved in tumor-induced angiogenesis and the metastatic dissemination of cancer cells, we hypothesized that it might be a target of oncogenic Gαq signaling via PDZ-RhoGEF. Consistent with this possibility, we found that Gαq Q209L drives full-length PDZ-RhoGEF and a DH-PH construct to interact with nucleotide-free RhoJ-G33A, a mutant with affinity for active RhoJ-GEFs. Gαq Q209L binding to PDZ-RhoGEF was mapped to the PH domain, which, as an isolated construct, attenuated the interaction of this mutant GTPase with PDZ-RhoGEF’s catalytic module (DH-PH domains). Expression of these catalytic domains caused contraction of endothelial cells and generated fine cell sprouts that were inhibited by co-expression of dominant negative RhoJ. Using relational data mining of uveal melanoma patient TCGA datasets, we got an insight into the signaling landscape that accompanies the Gαq/PDZ-RhoGEF/RhoJ axis. We identified three transcriptional signatures statistically linked with shorter patient survival, including GPCRs and signaling effectors that are recognized as vulnerabilities in cancer cell synthetic lethality datasets. In conclusion, we demonstrated that an oncogenic Gαq mutant enables the PDZ-RhoGEF DH-PH module to recognize RhoJ, suggesting an allosteric mechanism by which this constitutively active GTPase stimulates RhoJ via PDZ-RhoGEF. These findings highlight PDZ-RhoGEF and RhoJ as potential targets in tumors driven by mutant Gαq.
The Neurotoxic Effect of Environmental Temperature Variation in Adult Zebrafish (Danio rerio)
Authors: Maffioli Elisa , Nonnis Simona , Grassi Scalvini Francesca , Negri Armando , Tedeschi Gabriella , Toni Mattia
Published: October 2023

Neurotoxicity consists of the altered functionality of the nervous system caused by exposure to chemical agents or altered chemical–physical parameters. The neurotoxic effect can be evaluated from the molecular to the behavioural level. The zebrafish Danio rerio is a model organism used in many research fields, including ecotoxicology and neurotoxicology. Recent studies by our research group have demonstrated that the exposure of adult zebrafish to low (18 °C) or high (34 °C) temperatures alters their brain proteome and fish behaviour compared to control (26 °C). These results showed that thermal variation alters the functionality of the nervous system, suggesting a temperature-induced neurotoxic effect. To demonstrate that temperature variation can be counted among the factors that generate neurotoxicity, eight different protein datasets, previously published by our research group, were subjected to new analyses using an integrated proteomic approach by means of the Ingenuity Pathway Analysis (IPA) software (Release December 2022). The datasets consist of brain proteome analyses of wild type adult zebrafish kept at three different temperatures (18 °C, 26 °C, and 34 °C) for 4 days (acute) or 21 days (chronic treatment), and of BDNF+/− and BDNF−/− zebrafish kept at 26 °C or 34 °C for 21 days. The results (a) demonstrate that thermal alterations generate an effect that can be defined as neurotoxic (p value ≤ 0.05, activation Z score ≤ −2 or ≥2), (b) identify 16 proteins that can be used as hallmarks of the neurotoxic processes common to all the treatments applied and (c) provide three protein panels (p value ≤ 0.05) related to 18 °C, 34 °C, and BDNF depletion that can be linked to anxiety-like or boldness behaviour upon these treatments.
The Tumorigenicity of Breast Cancer Cells Is Reduced upon Treatment with Small Extracellular Vesicles Isolated from Heparin Treated Cell Cultures
Authors: Chen Yunliang , Scully Michael
Published: October 2023

As a member of the HPSG family, heparin is often used as a specific probe of their role in cell physiology; indeed, we have previously shown a reduction in the tumorigenicity of breast cancer cells when cultured in its presence. However, a partial reversal of the anti-tumorigenic effect occurred when the treated cells were cultured in fresh medium without heparin, which led us to consider whether a more persistent effect could be achieved by treatment of the cells with small extracellular vesicles (sEV) from heparin-treated cells. The tumorigenicity was analyzed using sEV isolated from the culture medium of heparin-treated MCF-7 and MDA-MB231 breast cancer cells (sEV-HT) or from conditioned medium following the termination of treatment (heparin discontinued, sEV-HD). Tumorigenicity was reduced in cells cultured in the presence of sEV-HT compared to that of cells cultured in the presence of sEV from untreated cells (sEV-Ctrl). sEV-HD were also observed to exert an anti-tumorigenic effect on the expression of pro-tumorigenic and cell cycle regulatory proteins, as well as signaling activities when added to fresh cultures of MCF-7 and MDA-MB231 cells. The anti-tumorigenic activity of the heparin-derived sEV may arise from observed changes in the miRNA content or from heparin, which was observed to be bound to the sEV. sEV may constitute a relatively stable reservoir of circulating heparin, allowing heparin activity to persist in the circulation even after therapy has been discontinued. These findings can be considered as a special additional pharmacological characteristic of heparin clinical therapy.
Blood Biomarkers as Prognostic Indicators for Neurological Injury in COVID-19 Patients: A Systematic Review and Meta-Analysis
Authors: Huang Zhiwei , Haile Kassahun , Gedefaw Lealem , Lau Benson Wui-Man , Jin Ling , Yip Shea Ping , Huang Chien-Ling
Published: October 2023

Coronavirus disease 2019 (COVID-19) has been linked to various neurological complications. This meta-analysis assessed the relationship between glial fibrillary acidic protein (GFAP) and neurofilament light chain (NfL) levels in the blood and neurological injury in COVID-19 patients. A comprehensive search of various databases was conducted until 18 August 2023, to find studies reporting GFAP and NfL blood levels in COVID-19 patients with neurological complications. GFAP and NfL levels were estimated between COVID-19 patients and healthy controls, and meta-analyses were performed using RevMan 5.4 software for analysis. In the 21 collected studies, it was found that COVID-19 patients had significantly higher levels of pooled GFAP (SMD = 0.52; 95% CI: 0.31, 0.73; p ≤ 0.001) and NfL (SMD = 0.60; 95% CI: 0.37, 0.82; p ≤ 0.001) when compared to the healthy controls. The pooled GFAP (SMD = 0.86; 95% CI: 0.26, 1.45; p ≤ 0.01) and NfL (SMD = 0.87; 95% CI: 0.48, 1.26; p ≤ 0.001) were significantly higher in non-survivors. These findings indicate a significant association between COVID-19 severity and elevated levels of GFAP and NfL, suggesting that GFAP and NfL could serve as potential diagnostic and prognostic markers for the early detection and monitoring of COVID-19-related neurological injuries.
Emerging Translational Research in Neurological and Psychiatric Diseases: From In Vitro to In Vivo Models
Authors: Tanaka Masaru , Szabó Ágnes , Vécsei László , Giménez-Llort Lydia
Published: October 2023

Revealing the underlying pathomechanisms of neurological and psychiatric disorders, searching for new biomarkers, and developing novel therapeutics all require translational research [...]
Plant Responses and Tolerance to Salt Stress: Physiological and Molecular Interventions 2.0
Authors: Hasanuzzaman Mirza , Fujita Masayuki
Published: October 2023

Environmental problems are pervasive and significantly impact a variety of plant species, which are affected by two broad types of conditions: abiotic and biotic stress [...]
Next-Generation Sequencing for Evaluating the Soil Nematode Diversity and Its Role in Composting Processes
Authors: Zapałowska Anita , Skwiercz Andrzej , Tereba Anna , Puchalski Czesław , Malewski Tadeusz
Published: October 2023

Biodiversity within composting systems involves a variety of microorganisms including nematodes. In the research, nematode populations were monitored within six simultaneously operating composting processes. These processes involved varying proportions of feedstock materials. The primary objective was to evaluate the consistency of nematode community succession patterns across the composting processes over a time of 3 months. During the study, samples were taken every month to isolate nematodes, determine the population density of the five trophic groups (per genus) and determine the dominant nematode species. It was shown that the bacterial-feeding community maintained dominance, while the fungus-feeding nematodes gradually increased in dominance as the maturation process progressed. The presence of predatory nematodes Mononchoides which were initially absent, along with the total absence of parasitic nematodes in the late stages of waste stabilization, serves as strong evidence for the reliable evaluation of the biodegradable waste processing level. Based on the obtained results, it is evident that the succession of nematode communities holds promise as a reliable method for evaluating compost maturity.