bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary Design Tool for Medium-Low-Power Gridded Ion Thrusters
Authors: Alifano Filippo , Panelli Mario , Battista Francesco
Published: May 2023

Gridded ion thrusters (GITs) are an established technology that, by covering a wide range of power class, allows one to accomplish a lot of space mission types. Many analysis tools and analytical models describing the physics of GITs are present in the open literature, while there is a lack of tools for preliminary design, considering the mission requirements (i.e., thrust or power). Thus, in this work, a tool that takes as input thrust or power and that combines analytical formulas, describing GITs’ physics; a curve-fitting approach, exploiting data from different ion thrusters present in the open literature; and an FEMM (finite element method magnetics) simulation has been developed and validated against known medium-low-power (<5 kW) gridded ion thrusters (e.g., NSTAR, XIPS, ETS-8). Some of the main outputs of the developed tool are its specific impulse, efficiencies, voltages, and propellant flow rate. The results obtained by the tool have been in good agreement with the real performance and working parameters of the thrusters selected for the validation, obtaining an average error of less than 5–10%. The tool has been also compared with a tool proposed in the literature as a possible design tool, which makes use of a simple macroscopic plasma-source simulation (SMPS) code with a genetic algorithm (GA) and obtains slightly more accurate results on average. Finally, the tool has been exploited for the design of a very low-power GIT (100 W) that is able to produce 2 mN of thrust, as the interest of the scientific community in miniaturizing electric engines has recently grown because they could enable new space missions.
Application of Elliptic Jerk Motion Profile to Cartesian Space Position Control of a Serial Robot
Authors: Bruzzone Luca , Stretti Daniele
Published: May 2023

The paper discusses the application of a motion profile with an elliptic jerk to Cartesian space position control of serial robots. This motion profile is obtained by means of a kinematic approach, starting from the jerk profile and then calculating acceleration, velocity and position by successive integrations. Until now, this profile has been compared to other motion laws (trapezoidal velocity, trapezoidal acceleration, cycloidal, sinusoidal jerk, modified sinusoidal jerk) considering single-input single-output systems. In this work, the comparison is extended to nonlinear multi-input multi-output systems, investigating the application to Cartesian space position control of serial robots. As case study, a 4-DOF SCARA-like architecture with elastic balancing is considered; both an integer-order and a fractional-order controller are applied. Multibody simulation results show that, independently of the controller, the behavior of the robot using the elliptic jerk profile is similar to the case of adopting the sinusoidal jerk and modified sinusoidal jerk laws, but with a slight reduction in the position error (−3.8% with respect to the sinusoidal jerk law and −0.8% with respect to the modified sinusoidal jerk law in terms of Integral Square Error) and of the control effort (−8.2% with respect to the sinusoidal jerk law and −1.3% with respect to the modified sinusoidal jerk law in terms of Integral Control Effort).
Topology Duration Optimization for UAV Swarm Network under the System Performance Constraint
Authors: Zhou Rui , Zhang Xiangyin , Song Deyu , Qin Kaiyu , Xu Limei
Published: May 2023

Network topology construction plays an important role in the application of large-scale unmanned aerial vehicle (UAV) swarm. Current researches usually perform the topology construction in terms of criteria of nodes energy consumption, transmission delay and network throughput, etc. However, another important criterion, the stability of swarm network topology, which is much critical for dynamic scenarios, has not been fully considered. In this paper, a novel topology construction method for UAV swarm network based on the criterion of topology duration is proposed. Specially, the topology construction of swarm network is formulated as an optimization problem of maximizing the topology duration while satisfying the constraints of certain network throughput, end-to-end delay, and nodes energy consumption. Then, a novel Group Trend Similarity based double-head Clustering method(GTSC) is employed to solve this problem, in which group similarity of movement, intra- and inter-cluster distance, node forwarding delay, and energy strategy are comprehensively taken into account. The proposed method is effective when used to perform the network topology construction for UAV swarm, which is verified by the simulation results. Furthermore, in comparison with representative algorithms, the proposed GTSC method exhibits better performance on topology duration, network throughput, end-to-end delay and energy consumption balance especially in a large-scale swarm scenarios.
An Interactive Augmented and Virtual Reality System for Managing Dental Anxiety among Young Patients: A Pilot Study
Authors: Alabduljabbar Reham , Almutawa Maha , Alkathiri Renad , Alqahtani Abeer , Alshamlan Hala
Published: May 2023

Dental anxiety is a common health problem among children. It creates major issues for patients, parents, and dental professionals. Children who cancel or otherwise miss their dental appointments generally do so due to fear of the unknown and lack of understanding of what they can expect from the environment and treatment when they arrive there. Some distraction interventions are already used by dental professionals, such as using clown doctors, watching cartoons, and utilizing the tell–show–do (TSD) technique. Still, the problem is common, and the fail to attend (FTA) rates at clinics are high. Familiarizing children with the dental setting and procedures in advance may help to manage their anxiety. This paper aims to help in managing children’s dental anxiety in a simple, attractive, and age-appropriate way through the use of augmented reality (AR) and virtual reality (VR) technologies. The developed system is named “Dr. Barea”. It targets Arabic-speaking children aged from 7 to 10 years old. It uses model–view–control (MVC) as its architectural design pattern. The proposed solution consists of three main sections: a 360° VR video that simulates a dental clinic environment, an educational description on dental tools using AR technology, and interactive educational stories that educate children about dental hygiene. The system performance was evaluated using unit, integration, performance, and user acceptance testing. The results demonstrate that the proposed solution, which performed reasonably, achieved the usability requirements and was engaging for learning information about dental hygiene. A feasibility study with 16 children was conducted to evaluate the effectiveness of the proposed system. The Child Fear Survey Schedule—Dental Subscale (CFSS-DS) was used to measure children’s dental anxiety level. The T test was used to evaluate the differences between groups, and Fisher’s exact test was used to compare the distributions of gender and age between the groups. The CFSS-DS index in the VR group decreased after dental consultation (35.04 ± 9.14 before consultation and 32.32 ± 8.32 after consultation, p = 0.041). The implications of this study shall be beneficial to patients, parents, and dental professionals.
Multi-Strategy Improved Flamingo Search Algorithm for Global Optimization
Authors: Jiang Shuhao , Shang Jiahui , Guo Jichang , Zhang Yong
Published: May 2023

To overcome the limitations of the Flamingo Search Algorithm (FSA), such as a tendency to converge on local optima and improve solution accuracy, we present an improved algorithm known as the Multi-Strategy Improved Flamingo Search Algorithm (IFSA). The IFSA utilizes a cube chaotic mapping strategy to generate initial populations, which enhances the quality of the initial solution set. Moreover, the information feedback model strategy is improved to dynamically adjust the model based on the current fitness value, which enhances the information exchange between populations and the search capability of the algorithm itself. In addition, we introduce the Random Opposition Learning and Elite Position Greedy Selection strategies to constantly retain superior individuals while also reducing the probability of the algorithm falling into a local optimum, thereby further enhancing the convergence of the algorithm. We evaluate the performance of the IFSA using 23 benchmark functions and verify its optimization using the Wilcoxon rank-sum test. The compared experiment results indicate that the proposed IFSA can obtain higher convergence accuracy and better exploration abilities. It also provides a new optimization algorithm for solving complex optimization problems.
Numerical Study of Mode Conversion between Fundamental Lamb and Rayleigh Waves
Authors: Vu Alex , Bond Leonard J. , Chakrapani Sunil K.
Published: May 2023

This article reports the findings of a numerical study of mode conversion between fundamental Lamb and Rayleigh waves and vice versa in quarter and half spaces. Fundamental Lamb wave (A0 and S0) propagation in a plate attached to a quarter space and the subsequent mode conversion to a Rayleigh wave was studied using finite element analysis. B-Scans show that a beat-like phenomenon can be observed for the R → L conversion and a generation length can be observed for the L → R conversion. The quarter-space model was also used to study the Rayleigh to Lamb mode conversion. Two hypotheses were developed based on the observed mode conversion efficiencies: (a) the main driving factor of mode conversion between Lamb and Rayleigh waves is the grazing incidence of bulk modes and (b) due to scattering and diffraction, the modes will require a generation length to stabilize in amplitude. Both hypotheses were tested and confirmed using numerical models, including a half-space model to study the diffraction of bulk waves from an incident Lamb wave. The results are of significance for nondestructive evaluation of complex structures where such structural discontinuities exist and it becomes important to understand the fundamental mode conversion phenomenon.
Spatiotemporal Variation Characteristics and Influencing Factors of Karst Cave Microclimate Environments: A Case Study in Shuanghe Cave, Guizhou Province, China
Authors: Xiong Yong , Zhou Zhongfa , Ding Shengjun , Zhang Heng , Huang Jing , Gong Xiaohuan , Su Dan
Published: May 2023

To systematically analyze the spatiotemporal heterogeneity, diurnal variation characteristics, and influencing factors of karst cave microclimate environments in Mahuang Cave, a cave in the Shuanghe Cave National Geological Park in Guizhou Province, China, was investigated. Monthly monitoring of meteorological and environmental parameters, such as wind speed, air pressure, humidity, and temperature indicators inside the cave and atmospheric temperature and precipitation outside the cave, was conducted from 2019 to 2021, as well as encrypted monitoring in August and December 2019. The results showed that: (1) The meteorological parameters of Mahuang Cave exhibited seasonal characteristics and cyclical interannual variation. Cave wind speed, relative humidity, and temperature were high in summer and autumn and lowest in winter, whereas cave air pressure was high in winter and low in summer. The atmospheric temperature outside the cave was the main controlling factor. (2) On a short time scale, the dewpoint and temperature of Mahuang Cave did not change significantly, and an abrupt change phenomenon occurred mostly around noon. The warm season was more sensitive than the cold season, and the closer to the entrance of the cave, the stronger the response. (3) In terms of spatial distribution, the overall microclimate factors of Mahuang Cave became increasingly stable and entered a constant state with the increasing depth of the cave passage. The related effects of cave morphology and structure, the physical environment of the cave passage, air movement, and groundwater dynamics were important factors leading to an abrupt change phenomenon in cave microclimates. Local meteorological conditions and cave geometry controlled the temporal variability and spatial heterogeneity of the cave microclimate environment.
The Impacts of the Application of the Ensemble Optimal Interpolation Method in Global Ocean Wave Data Assimilation
Authors: Wu Mengmeng , Wang Hui , Wan Liying , Wang Juanjuan , Wang Yi , Wang Jiuke
Published: May 2023

The ensemble optimal interpolation method was used in this study to conduct an examination of the assimilations of significant wave height (SWH) data from HY-2A satellite altimeter based on the WAVEWATCH III global ocean wave model. The results suggested that the ensemble optimal interpolation method using HY-2A SWH data played a positive role in enhancing the accuracy of the global ocean wave simulations and could effectively improve the deviations of SWH in the simulation processes. The root mean square errors of the NDBC buoy inspections were improved by 7 to 44% after the assimilation, and those of China’s offshore buoy inspections were improved by 3 to 11% after the assimilation. It was observed that the farther the buoys were from the shore, the better the effects of the assimilation improvements. The root mean square errors of the Jason-2 satellite data validations were improved by 17% after the assimilation, with monthly improvements of 8–25%. The improvements occurred in most of the global oceans, particularly in the Southern Ocean, the Eastern Pacific Ocean and the Indian Ocean. The results obtained in this research can be used as a reference for the operational applications of China’s ocean satellite data in ocean wave data assimilation and prediction.
Seismogenic Field in the Ionosphere before Two Powerful Earthquakes: Possible Magnitude and Observed Ionospheric Effects (Case Study)
Authors: Hegai Valery , Zeren Zhima , Pulinets Sergey
Published: May 2023

A retrospective analysis of complex geophysical data around the time of the two most powerful earthquakes that occurred in Alaska and had magnitudes M = 8.2 (29 July 2021) and M = 9.2 (28 March 1964), respectively, is carried out. The aim of the research is to assess the maximum possible magnitude of the electric field of a seismogenic nature that penetrated the ionosphere/plasmasphere, which could cause the ionospheric effects observed experimentally. Theoretical calculations have shown that under the geophysical conditions that existed before these earthquakes (favorable for the penetration of the seismogenic field into the ionosphere), the maximum value of a quasi-static electric seismogenic field in the ionosphere, perpendicular to geomagnetic field lines (tens of hours/units of days before the earthquake) for earthquakes with magnitudes M = 8–9 could reach 1–2 mV/m. Such values are sufficient for the formation of a plasmaspheric ULF-ELF-VLF-duct, which is formed in the vicinity of the geomagnetic field-line passing through the epicenter of the earthquake under the influence of a seismogenic electric field that penetrated into the ionosphere/plasmasphere. This leads to an anomalous amplification of the captured ULF-ELF-VLF waves, ULF (DC-16 Hz), ELF (6 Hz–2.2 kHz), VLF (1.8–20 kHz), not only above the epicenter of the future earthquake, but also at the point magnetically conjugated with the epicenter of the earthquake, testifying to the formation of such a duct, stretched along the geomagnetic field from one hemisphere to another, and formed on closed L-shells shortly before the earthquake. This result is confirmed by the measurements of the mission of the CSES satellite (China-Seismo-Electromagnetic Satellite) for the 29 July 2021 earthquake with magnitude M = 8.2.
On Hermite–Hadamard-Type Inequalities for Functions Satisfying Second-Order Differential Inequalities
Authors: Aldawish Ibtisam , Jleli Mohamed , Samet Bessem
Published: May 2023

Hermite–Hadamard inequality is a double inequality that provides an upper and lower bounds of the mean (integral) of a convex function over a certain interval. Moreover, the convexity of a function can be characterized by each of the two sides of this inequality. On the other hand, it is well known that a twice differentiable function is convex, if and only if it admits a nonnegative second-order derivative. In this paper, we obtain a characterization of a class of twice differentiable functions (including the class of convex functions) satisfying second-order differential inequalities. Some special cases are also discussed.
Sustainable Approach for the Development of TiO2-Based 3D Electrodes for Microsupercapacitors
Authors: Poirot Nathalie , Gabard Marie , Boufnichel Mohamed , Omnée Rachelle , Raymundo-Piñero Encarnacion
Published: May 2023

This study reports a sustainable approach for developing electrodes for microsupercapacitors. This approach includes the synthesis of TiO2 nanoparticles via a green sol–gel method and the deposition of thin films of that electrochemically active material on three-dimensional (3D) Si substrates with a high area enlargement factor (AEF) via a simple, fast, and inexpensive spin-coating pathway. The thickness of the film was first optimized via its deposition over two-dimensional (2D) substrates to achieve high capacitances to provide high energy density but also to deliver a good rate capability to ensure the power density required for a supercapacitor device. A film thickness of ~120 nm realizes the best compromise between the electronic/ionic conductivity and capacitance in a supercapacitor device. Such layers of TiO2 were successfully coated onto 3D microstructured substrates with different architectures, such as trenches and pillars, and different aspect ratios. The spin-coating-based route developed here has been established to be superior as, on the one hand, a conformal deposition can be achieved over high AEF subtracts, and on the other hand, the 3D electrodes present higher surface capacitances than those obtained using other deposition techniques. The rate capability and appreciable cyclability ensure a reliable supercapacitor behavior.
Parameter Mapping Sonification of Human Olfactory Thresholds
Authors: Boevé Jean-Luc , Giot Rudi
Published: May 2023

An objective of chemical ecology is to understand the chemical diversity across and within species, as well as the bioactivity of chemical compounds. We previously studied defensive volatiles from phytophagous insects that were subjected to parameter mapping sonification. The created sounds contained information about the repellent bioactivity of the volatiles, such as the repellence from the volatiles themselves when tested against live predators. Here, we applied a similar sonification process to data about human olfactory thresholds. Randomized mapping conditions were used and a peak sound pressure, Lpeak, was calculated from each audio file. The results indicate that Lpeak values were significantly correlated with the olfactory threshold values (e.g., rS = 0.72, t = 10.19, p < 0.001, Spearman rank-order correlation; standardized olfactory thresholds of 100 volatiles). Furthermore, multiple linear regressions used the olfactory threshold as a dependent variable. The regressions revealed that the molecular weight, the number of carbon and oxygen atoms, as well as the functional groups aldehyde, acid, and (remaining) double bond were significant determinants of the bioactivity, while the functional groups ester, ketone, and alcohol were not. We conclude that the presented sonification methodology that converts chemicals into sound data allows for the study of their bioactivities by integrating compound characteristics that are easily accessible.
PI3K/AKT/mTOR Signaling Pathway in HPV-Driven Head and Neck Carcinogenesis: Therapeutic Implications
Authors: Aguayo Francisco , Perez-Dominguez Francisco , Osorio Julio C. , Oliva Carolina , Calaf Gloria M.
Published: May 2023

High-risk human papillomaviruses (HR-HPVs) are the causal agents of cervical, anogenital and a subset of head and neck carcinomas (HNCs). Indeed, oropharyngeal cancers are a type of HNC highly associated with HR-HPV infections and constitute a specific clinical entity. The oncogenic mechanism of HR-HPV involves E6/E7 oncoprotein overexpression for promoting cell immortalization and transformation, through the downregulation of p53 and pRB tumor suppressor proteins, among other cellular targets. Additionally, E6/E7 proteins are involved in promoting PI3K/AKT/mTOR signaling pathway alterations. In this review, we address the relationship between HR-HPV and PI3K/AKT/mTOR signaling pathway activation in HNC with an emphasis on its therapeutic importance.
Comparative Evaluation of Chlorella vulgaris and Anabaena variabilis for Phycoremediation of Polluted River Water: Spotlighting Heavy Metals Detoxification
Authors: Ahammed Md. Shakir , Baten Md. Abdul , Ali Muhammad Aslam , Mahmud Shahin , Islam Md. Sirajul , Thapa Bhim Sen , Islam Md. Aminul , Miah Md. Alim , Tusher Tanmoy Roy
Published: May 2023

This study investigated the phycoremediation abilities of Chlorella vulgaris (microalga) and Anabaena variabilis (cyanobacterium) for the detoxification of polluted river water. Lab-scale phycoremediation experiments were conducted for 20 days at 30 °C using the microalgal and cyanobacterial strains and water samples collected from the Dhaleswari river in Bangladesh. The physicochemical properties such as electrical conductivity (EC), total dissolved solids (TDS), biological oxygen demand (BOD), hardness ions, and heavy metals of the collected water samples indicated that the river water is highly polluted. The results of the phycoremediation experiments demonstrated that both microalgal and cyanobacterial species significantly reduced the pollutant load and heavy metal concentrations of the river water. The pH of the river water was significantly raised from 6.97 to 8.07 and 8.28 by C. vulgaris and A. variabilis, respectively. A. variabilis demonstrated higher efficacy than C. vulgaris in reducing the EC, TDS, and BOD of the polluted river water and was more effective at reducing the pollutant load of SO42− and Zn. In regard to hardness ions and heavy metal detoxification, C. vulgaris performed better at removing Ca2+, Mg2+, Cr, and Mn. These findings indicate that both microalgae and cyanobacteria have great potential to remove various pollutants, especially heavy metals, from the polluted river water as part of a low-cost, easily controllable, environmentally friendly remediation strategy. Nevertheless, the composition of polluted water should be assessed prior to the designing of microalgae- or cyanobacteria-based remediation technology, since the pollutant removal efficiency is found to be species dependent.
Glucose Transporter Glut1-Dependent Metabolic Reprogramming Regulates Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages
Authors: Cornwell Alex , Ziółkowski Hubert , Badiei Alireza
Published: May 2023

This study investigated the critical role of Glut1-mediated glucose metabolism in the inflammatory response of macrophages, which are energy-intensive cells within the innate immune system. Inflammation leads to increased Glut1 expression, ensuring sufficient glucose uptake to support macrophage functions. We demonstrated that using siRNA to knock down Glut1 reduces the expression of various pro-inflammatory cytokines and markers, such as IL-6, iNOS, MHC II/CD40, reactive oxygen species, and the hydrogen sulfide (H2S)-producing enzyme cystathionine γ-lyase (CSE). Glut1 activates a pro-inflammatory profile through a nuclear factor (NF)-κB, while silencing Glut1 can prevent lipopolysaccharide (LPS)-induced IκB degradation, blocking NF-κB activation. Glut1’s role in autophagy, an essential process for macrophage functions such as antigen presentation, phagocytosis, and cytokine secretion, was also measured. The findings show that LPS stimulation decreases autophagosome formation, but Glut1 knockdown reverses this effect, increasing autophagy beyond control levels. The study highlights Glut1’s importance in macrophage immune responses and its regulation of apoptosis during LPS stimulation. Knocking down Glut1 negatively impacts cell viability and mitochondrial intrinsic pathway signaling. These findings collectively suggest that targeting macrophage glucose metabolism through Glut1 could potentially serve as a target for controlling inflammation.
Identification of Potential Inflammation-Related Genes and Key Pathways Associated with Complex Regional Pain Syndrome
Authors: Zhu He , Wen Bei , Xu Li , Huang Yuguang
Published: May 2023

Complex regional pain syndrome (CRPS) is a chronic pain that affects the extremities after a trauma or nerve injury with no definite established treatment. The mechanisms mediating CRPS are not completely elucidated. Thus, we conducted a bioinformatics analysis to identify hub genes and key pathways to determine strategies for more effective treatments of CRPS. Finally, there is only one expression profile of GSE47063 in terms of homo sapiens-based CRPS from the Gene Expression Omnibus (GEO) database, which included four patients and five controls. We explored the differentially expressed genes (DEGs) in the dataset and conducted Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the potential hub genes. A protein–protein interaction (PPI) network was also established; then, according to the score of each hub genes, we used R software to draw the nomogram model to predict the rate of CRPS. Furthermore, GSEA analysis was estimated and assessed by the normalized enrichment score (NES). From the GO and KEGG analysis, we identified the top five hub genes (MMP9, PTGS2, CXCL8, OSM, TLN1); all of the selected DEGs were mainly enriched in their inflammatory response. In addition, the GSEA analysis showed complement and coagulation cascades also play an important role in CRPS. This study, to our knowledge, is the first to conduct further PPI network and GSEA analyses. Thus, targeting excessive inflammation could offer new therapeutic methods for CRPS and related physical and psychiatric disorders.
Legionella Research: Still Many Miles to Go
Authors: Belyi Yury
Published: May 2023

Legionella is a widespread Gram-negative bacterium occurring in water reservoirs and soils [...]
Mice Mutated in the Third Fibronectin Domain of L1 Show Enhanced Hippocampal Neuronal Cell Death, Astrogliosis and Alterations in Behavior
Authors: Congiu Ludovica , Granato Viviana , Jakovcevski Igor , Kleene Ralf , Fernandes Luciana , Freitag Sandra , Kneussel Matthias , Schachner Melitta , Loers Gabriele
Published: May 2023

Adhesion molecules play major roles in cell proliferation, migration, survival, neurite outgrowth and synapse formation during nervous system development and in adulthood. The neural cell adhesion molecule L1 contributes to these functions during development and in synapse formation and synaptic plasticity after trauma in adulthood. Mutations of L1 in humans result in L1 syndrome, which is associated with mild-to-severe brain malformations and mental disabilities. Furthermore, mutations in the extracellular domain were shown to cause a severe phenotype more often than mutations in the intracellular domain. To explore the outcome of a mutation in the extracellular domain, we generated mice with disruption of the dibasic sequences RK and KR that localize to position 858RKHSKR863 in the third fibronectin type III domain of murine L1. These mice exhibit alterations in exploratory behavior and enhanced marble burying activity. Mutant mice display higher numbers of caspase 3-positive neurons, a reduced number of principle neurons in the hippocampus, and an enhanced number of glial cells. Experiments suggest that disruption of the dibasic sequence in L1 results in subtle impairments in brain structure and functions leading to obsessive-like behavior in males and reduced anxiety in females.
Disturbances in Switching between Canonical and Non-Canonical Wnt Signaling Characterize Developing and Postnatal Kidneys of Dab1−/− (yotari) Mice
Authors: Perutina Ilija , Kelam Nela , Maglica Mirko , Racetin Anita , Ogorevc Marin , Filipović Natalija , Katsuyama Yu , Mišković Josip , Vukojević Katarina
Published: May 2023

This study aims to determine the protein expression patterns of acetylated α-tubulin, inversin, dishevelled-1, Wnt5a/b, and β-catenin in developing (E13.5 and E15.5) and early postnatal (P4 and P14) kidneys of Dab1−/− (yotari) mice, their role in regulating the Wnt signaling pathway, and the possible relation to congenital anomalies of kidney and urinary tract (CAKUT). The analysis of target protein co-expression, observed in the renal vesicles/immature glomeruli, ampullae/collecting ducts, convoluted tubules, metanephric mesenchyme of developing kidneys, but proximal convoluted tubules, distal convoluted tubules and glomeruli of postnatal kidneys, was performed using double immunofluorescence and semi-quantitative methods. The overall expression of acetylated α-tubulin and inversin during normal kidney development increases with higher expression in yotari mice as the kidney acquires mature morphology. An increase in β-catenin and cytosolic DVL-1 levels, indicating a switch from non-canonical to canonical Wnt signaling, is found in the postnatal kidney of yotari mice. In contrast, healthy mouse kidney expresses inversin and Wnt5a/b in the postnatal period, thus activating non-canonical Wnt signaling. Target protein expression patterns in kidney development and the early postnatal period observed in this study could indicate that switching between canonical and non-canonical Wnt signaling is crucial for normal nephrogenesis, while the defective Dab1 gene product in yotari mice may promote CAKUT due to interfering with this process.
Multimodal Neuromonitoring and Neurocritical Care in Swine to Enhance Translational Relevance in Brain Trauma Research
Authors: O’Donnell John C. , Browne Kevin D. , Kvint Svetlana , Makaron Leah , Grovola Michael R. , Karandikar Saarang , Kilbaugh Todd J. , Cullen D. Kacy , Petrov Dmitriy
Published: May 2023

Neurocritical care significantly impacts outcomes after moderate-to-severe acquired brain injury, but it is rarely applied in preclinical studies. We created a comprehensive neurointensive care unit (neuroICU) for use in swine to account for the influence of neurocritical care, collect clinically relevant monitoring data, and create a paradigm that is capable of validating therapeutics/diagnostics in the unique neurocritical care space. Our multidisciplinary team of neuroscientists, neurointensivists, and veterinarians adapted/optimized the clinical neuroICU (e.g., multimodal neuromonitoring) and critical care pathways (e.g., managing cerebral perfusion pressure with sedation, ventilation, and hypertonic saline) for use in swine. Moreover, this neurocritical care paradigm enabled the first demonstration of an extended preclinical study period for moderate-to-severe traumatic brain injury with coma beyond 8 h. There are many similarities with humans that make swine an ideal model species for brain injury studies, including a large brain mass, gyrencephalic cortex, high white matter volume, and topography of basal cisterns, amongst other critical factors. Here we describe the neurocritical care techniques we developed and the medical management of swine following subarachnoid hemorrhage and traumatic brain injury with coma. Incorporating neurocritical care in swine studies will reduce the translational gap for therapeutics and diagnostics specifically tailored for moderate-to-severe acquired brain injury.
Bacterial Lux Biosensors in Genotoxicological Studies
Authors: Abilev Serikbai K. , Igonina Elena V. , Sviridova Darya A. , Smirnova Svetlana V.
Published: May 2023

The aim of this study was to assess the applicability of the bacterial lux biosensors for genotoxicological studies. Biosensors are the strains of E. coli MG1655 carrying a recombinant plasmid with the lux operon of the luminescent bacterium P. luminescens fused with the promoters of inducible genes: recA, colD, alkA, soxS, and katG. The genotoxicity of forty-seven chemical compounds was tested on a set of three biosensors pSoxS-lux, pKatG-lux and pColD-lux, which allowed us to estimate the oxidative and DNA-damaging activity of the analyzed drugs. The comparison of the results with the data on the mutagenic activity of these drugs from the Ames test showed a complete coincidence of the results for the 42 substances. First, using lux biosensors, we have described the enhancing effect of the heavy non-radioactive isotope of hydrogen deuterium (D2O) on the genotoxicity of chemical compounds as possible mechanisms of this effect. The study of the modifying effect of 29 antioxidants and radioprotectors on the genotoxic effects of chemical agents showed the applicability of a pair of biosensors pSoxS-lux and pKatG-lux for the primary assessment of the potential antioxidant and radioprotective activity of chemical compounds. Thus, the results obtained showed that lux biosensors can be successfully used to identify potential genotoxicants, radioprotectors, antioxidants, and comutagens among chemical compounds, as well as to study the probable mechanism of genotoxic action of test substance.
Glabridin Therapy Reduces Chronic Allodynia, Spinal Microgliosis, and Dendritic Spine Generation by Inhibiting Fractalkine-CX3CR1 Signaling in a Mouse Model of Tibial Fractures
Authors: Long Juan , Liu Hongbing , Qiu Zhimin , Xiao Zhong , Lu Zhongqiu
Published: May 2023

Patients undergoing bone fractures frequently suffer from irritating chronic pain after orthopedic repairs. Chemokine-mediated interactions between neurons and microglia are important steps for neuroinflammation and excitatory synaptic plasticity during the spinal transmission of pathological pain. Recently, glabridin, the main bioactive component of licorice, has been shown to exhibit anti-nociceptive and neuroprotective properties for inflammatory pain. This present study evaluated the therapeutic potential of glabridin and its analgesic mechanisms using a mouse model of tibial fracture-associated chronic pain. Repetitive injections of glabridin were delivered spinally daily for 4 continuous days from days 3 to 6 after the fractures. Herein, we discovered that repeated administrations of glabridin (10 and 50 μg, but not 1 μg) could prevent prolonged cold allodynia and mechanical allodynia following bone fractures. A single intrathecal intervention with glabridin (50 μg) relieved an existing chronic allodynia two weeks following the fracture surgeries. Systemic therapies with glabridin (intraperitoneal; 50 mg kg−1) were protective against long-lasting allodynia caused by fractures. Furthermore, glabridin restricted the fracture-caused spinal overexpressions of the chemokine fractalkine and its receptor CX3CR1, as well as the elevated number of microglial cells and dendritic spines. Strikingly, glabridin induced the inhibition of pain behaviors, microgliosis, and spine generation, which were abolished with the co-administration of exogenous fractalkine. Meanwhile, the exogenous fractalkine-evoked acute pain was compensated after microglia inhibition. Additionally, spinal neutralization of fractalkine/CX3CR1 signaling alleviated the intensity of postoperative allodynia after tibial fractures. These key findings identify that glabridin therapies confer protection against inducing and sustaining fracture-elicited chronic allodynia by suppressing fractalkine/CX3CR1-dependent spinal microgliosis and spine morphogenesis, suggesting that glabridin is a promising candidate in the translational development of chronic fracture pain control.
Reversal Training Discloses Gender Differences in a Spatial Memory Task in Humans
Authors: Tascón Laura , León Irene , Fernández Rubén , Cimadevilla José Manuel
Published: May 2023

Over the past few years, spatial memory has been studied using virtual-reality-based tasks. Reversal learning has been widely used in spatial orientation tasks for testing, among other things, new learning and flexibility. By means of a reversal-learning protocol, we assessed spatial memory in men and women. A total of sixty participants (half of them women) performed a task that included two phases: during the acquisition phase, participants were asked to find one or three rewarded positions in the virtual room across ten trials. During the reversal phase, the rewarded boxes were moved to a new position and maintained for four trials. The results showed that men and women differed in the reversal phase, with men outperforming women in high demanding conditions. Dissimilarities in several cognitive abilities between both genders are the base of these differences and are discussed.
Neural Responses to a Working Memory Task in Acute Depressed and Remitted Phases in Bipolar Patients
Authors: Kopf Juliane , Glöckner Stefan , Althen Heike , Cevada Thais , Schecklmann Martin , Dresler Thomas , Kittel-Schneider Sarah , Reif Andreas
Published: May 2023

(1) Cognitive impairments such as working memory (WM) deficits are amongst the most common dysfunctions characterizing bipolar disorder (BD) patients, severely contributing to functional impairment. We aimed to investigate WM performance and associated brain activation during the acute phase of BD and to observe changes in the same patients during remission. (2) Frontal brain activation was recorded using functional near-infrared spectroscopy (fNIRS) during n-back task conditions (one-back, two-back and three-back) in BD patients in their acute depressive (n = 32) and remitted (n = 15) phases as well as in healthy controls (n = 30). (3) Comparison of BD patients during their acute phase with controls showed a trend (p = 0.08) towards lower dorsolateral prefrontal cortex (dlPFC) activation. In the remitted phase, BD patients showed lower dlPFC and ventrolateral prefrontal cortex (vlPFC) activation (p = 0.02) compared to controls. No difference in dlPFC and vlPFC activation between BD patients’ phases was found. (4) Our results showed decreased working memory performance in BD patients during the working memory task in the acute phase of disease. Working memory performance improved in the remitted phase of the disease but was still particularly attenuated for the more demanding conditions.
Surgical Treatment of Pediatric Incidentally Found Brain Tumors: A Single-Center Experience
Authors: Antkowiak Lukasz , Zimny Mikolaj , Starszak Krzysztof , Sordyl Ryszard , Mandera Marek
Published: May 2023

There remains much debate about the correct management of incidentally found brain tumors in the pediatric population. This study aimed to evaluate the efficacy and safety of surgical treatment of incidentally found pediatric brain tumors. A retrospective analysis of pediatric patients who underwent surgical resection of incidentally found brain tumors between January 2010 and April 2016 was performed. A total of seven patients were included. The median age at the time of diagnosis was 9.7 years. The reasons for performing neuroimaging were as follows: impeded speech development (n = 2), shunt control (n = 1), paranasal sinuses control (n = 1), behavior changes (n = 1), head trauma (n = 1), and preterm birth (n = 1). Five patients underwent gross total tumor resection (71.4%), while subtotal resection was performed in two patients (28.6%). There was no surgery-related morbidity. Patients were followed up for a mean of 79 months. One patient with atypical neurocytoma experienced tumor recurrence 45 months following primary resection. All patients remained neurologically intact. The majority of pediatric incidentally found brain tumors were histologically benign. Surgery remains a safe therapeutic approach associated with favorable long-term outcomes. Considering the expected long lifetime of pediatric patients, as well as the psychological burden associated with having a brain tumor as a child, surgical resection can be considered an initial approach.
Cerebral Amyloid Angiopathy-Related Inflammation (CAA-rI): Three Heterogeneous Case Reports and a Focused Literature Review
Authors: Bozovic Ivo , Jeremic Marta , Pavlovic Aleksandra , Jovanovic Carna , Kresojevic Nikola , Vojvodic Nikola , Jovanovic Dejana , Sokic Dragoslav , Mijajlovic Milija
Published: May 2023

Cerebral amyloid angiopathy-related inflammation (CAA-rI) is a largely reversible, subacute encephalopathy, which is considered as a rare variant of cerebral amyloid angiopathy (CAA). Although the diagnosis of this inflammatory vasculopathy is generally clinico-pathologic, a probable or possible diagnosis can often be established based on current clinico-radiological diagnostic criteria. This is important since CAA-rI is considered as a treatable disorder, which most commonly occurs in the elderly population. Behavioral changes and cognitive deterioration are highlighted as the most common clinical signs of CAA-rI, followed by a heterogeneous spectrum of typical and atypical clinical presentations. However, despite the well-established clinical and radiological features incorporated in the current diagnostic criteria for this CAA variant, this rare disorder is still insufficiently recognized and treated. Here, we have shown three patients diagnosed with probable CAA-rI, with significant heterogeneity in the clinical and neuroradiological presentations, followed by different disease courses and outcomes after the introduction of immunosuppressive treatment. Moreover, we have also summarized up-to-date literature data about this rare, yet underdiagnosed, immune-mediated vasculopathy.
Comparison of Analgesic Efficacy between Epidural and Perineural Administration of Autologous Conditioned Serum in the Conservative Treatment of Low Back Pain Due to Lumbar Degenerative Disc Disease: A Randomized, Open-Label, Controlled Clinical Trial
Authors: Godek Piotr , Szczepanowska-Wolowiec Beata , Golicki Dominik
Published: May 2023

Lumbar degenerative disc disease (LDDD) is widely acknowledged as a significant contributor to low back pain (LBP), which is a prevalent and debilitating health condition affecting millions of individuals worldwide. The pathogenesis of LDDD and associated pain mechanisms are thought to be mediated by inflammatory mediators. Autologous conditioned serum (ACS, Orthokine) may be used for symptomatic treatment of LBP due to LDDD. This study aimed to compare the analgesic efficacy and safety of two routes of ACS administration, perineural (periarticular) and epidural (interlaminar), in the conservative treatment of LBP. This study used an open-label, randomized, controlled trial protocol. A group of 100 patients were enrolled in the study and randomly allocated into two comparative groups. Group A (n = 50) received the epidural (interlaminar) approach—2 ultrasound-guided injections as control intervention (each containing two doses of ACS—8 mL). Group B (n = 50) received the perineural (periarticular) approach—2 ultrasound-guided injections as experimental intervention at 7-day intervals (the same volume of ACS). Assessments consisted of an initial assessment (IA) and control assessments at 4 (T1), 12 (T2), and 24 (T3) weeks after the last intervention. Primary outcomes comprised Numeric Rating Scale (NRS), Oswestry Disability Index (ODI), Roland Morris Questionnaire (RMQ), and Euro Quality of Life—5 Dimensions–5 Levels (EQ-5D-5L): Index, Visual Analogue Scale (VAS), and Level Sum Score (LSS). Secondary outcomes included differences between groups in specific endpoints for the above-mentioned questionnaires. In conclusion, this study revealed that both perineural (periarticular) and epidural ACS injections tended to perform in a very similar way. Both routes of Orthokine application show significant improvement in the primary clinical parameters, such as pain and disability, and therefore, both methods can be considered equally effective in managing LBP due to LDDD.
Motor-Related Mu/Beta Rhythm in Older Adults: A Comprehensive Review
Authors: Inamoto Takashi , Ueda Masaya , Ueno Keita , Shiroma China , Morita Rin , Naito Yasuo , Ishii Ryouhei
Published: May 2023

Mu rhythm, also known as the mu wave, occurs on sensorimotor cortex activity at rest, and the frequency range is defined as 8–13Hz, the same frequency as the alpha band. Mu rhythm is a cortical oscillation that can be recorded from the scalp over the primary sensorimotor cortex by electroencephalogram (EEG) and magnetoencephalography (MEG). The subjects of previous mu/beta rhythm studies ranged widely from infants to young and older adults. Furthermore, these subjects were not only healthy people but also patients with various neurological and psychiatric diseases. However, very few studies have referred to the effect of mu/beta rhythm with aging, and there was no literature review about this theme. It is important to review the details of the characteristics of mu/beta rhythm activity in older adults compared with young adults, focusing on age-related mu rhythm changes. By comprehensive review, we found that, compared with young adults, older adults showed mu/beta activity change in four characteristics during voluntary movement, increased event-related desynchronization (ERD), earlier beginning and later end, symmetric pattern of ERD and increased recruitment of cortical areas, and substantially reduced beta event-related desynchronization (ERS). It was also found that mu/beta rhythm patterns of action observation were changing with aging. Future work is needed in order to investigate not only the localization but also the network of mu/beta rhythm in older adults.
Parental Phubbing and Adolescent Depressive Symptoms during COVID-19: A Serial Meditating Model
Authors: Yang Wencheng , Tang Lu , Shen Xiangping , Niu Gengfeng , Shi Xiaohan , Jin Siyu , Zhang Yumang , Yuan Zihui
Published: May 2023

Background: During the COVID-19 pandemic, depressive symptoms, a common emotional problem among adolescents, have become more prominent. Regarding the influencing factors of adolescent depressive symptoms, it is widely accepted that parents’ problematic cellphone use around the family (specifically parental phubbing) is a strong predictive factor for the development of depressive symptoms among adolescents. Notably, the COVID-19 pandemic caused a sharp increase in the number of individuals with depressive symptoms, and the negative consequences of parental phubbing and depressive symptoms might have been exacerbated. Accordingly, this study aimed to examine the association between parental phubbing and adolescent depressive symptoms as well as their underlying mechanism. Method: To test our hypotheses, we conducted an offline/online survey with 614 adolescents in Central China from May to June 2022, which corresponded to a period of strict lockdowns in some areas due to the outbreak of the Omicron variant. The participants completed a set of measures, including a technology interference questionnaire, a parent–child relationship scale, a self-concept clarity scale, and the depressive symptoms scale. Results: Parental phubbing was positively associated with adolescent depressive symptoms; the parent–child relationship and self-concept clarity could independently mediate this relationship; and the parent–child relationship and self-concept clarity were also serial mediators in this association. These findings extend previous research by highlighting the impact of parental technology use on their children and the underlying mechanism explaining adolescent depressive symptoms. They provide practical recommendations for parents to prioritize fostering a positive family environment and minimizing phubbing behaviors to enhance adolescent development, particularly in the context of the COVID-19 pandemic.