bluuebunny/crossref_metadata_2025 huggingface dataset:
Fungi in Microbial Culture Collections and Their Metabolites
Authors: Vasilenko Alexander , Ivanushkina Natalya , Kochkina Galina , Ozerskaya Svetlana
Published: June 2022

This study presents the results of a comparative analysis of the fungal diversity in the world system of microbial culture collections on one side with a variety of known fungal producers on the other side. The main VKM databases used are FungalDC and Metabolites of Fungi and the central point of analysis is the fungal ability to synthesize promising metabolites for applied use. It indicates that the option of obtaining new promising strains from the collection funds is still underestimated by the scientific community. In particular, it is shown that no more than 3% of the total fungal species fund contained in culture collections are used practically. It is possible that their use will considerably expand the range of studied strains and lead to the acquisition of new scientifically significant data.
Detection of Novel Biallelic Causative Variants in COL7A1 Gene by Whole-Exome Sequencing, Resulting in Congenital Recessive Dystrophic Epidermolysis Bullosa in Three Unrelated Families
Authors: Fozia Fozia , Nazli Rubina , Alrashed May Mohammed , Ghneim Hazem K. , Haq Zia Ul , Jabeen Musarrat , Alam Khan Sher , Ahmad Ijaz , Bourhia Mohammed , Aboul-Soud Mourad A. M.
Published: June 2022

Background: Dystrophic Epidermolysis bullosa (DEB) is a rare, severe subtype of epidermolysis bullosa (EB), characterized by blisters and miliary rashes of the skin. Dystrophic EB (DEB) includes variants inherited both in an autosomal-dominant or autosomal-recessive manner. Recessive dystrophic EB (RDEB) is divided into many subtypes and prevails as a result of biallelic genetic mutations in COL7A1 gene encoding type VII collagen, a major stabilizing molecule of the dermo-epidermal junction. The blister formation is mainly due to the variable structural and functional impairment of anchoring fibrils in VII collagen (COLVII), responsible for the adhesion of the epidermis to the dermis. Method: Three Pakistani families (A, B and C) affected with congenital dystrophic epidermolysis bullosa were recruited in the present study. The whole-exome sequencing (WES) approach was utilized for the detection of the pathogenic sequence variants in probands. The segregation of these variants in other participants was confirmed by Sanger sequencing. Results: This study identified a novel missense variant c.7034G>A, p. Gly2345Asp in exon 91, a novel Frameshift mutation c.385del (p. His129MetfsTer18) in a homozygous form in exon no 3, and a previously known nonsense variation (c.1573 C>T; p. Arg525Ter) in exon 12 of COL7A1 gene in families A, B, and C, respectively, as causative mutations responsible for dystrophic epidermolysis bullosa in these families. Conclusion: Our study validates the involvement of the COL7A1 gene in the etiology of dystrophic epidermolysis bullosa. It further expands the COL7A1 gene mutation database and provides an additional scientific basis for diagnosis, genetic counseling, and prognosis purposes for EB patients.
Practices of Linked Open Data in Archaeology and Their Realisation in Wikidata
Authors: Schmidt Sophie C. , Thiery Florian , Trognitz Martina
Published: June 2022

In this paper, we introduce Linked Open Data (LOD) in the archaeological domain as a means to connect dispersed data sources and enable cross-querying. The technology behind the design principles and how LOD can be created and published is described to enable less-familiar researchers to understand the presented benefits and drawbacks of LOD. Wikidata is introduced as an open knowledge hub for the creation and dissemination of LOD. Different actors within archaeology have implemented LOD, and we present which challenges have been and are being addressed. A selection of projects showcases how Wikidata is being used by archaeologists to enrich and open their databases to the general public. With this paper, we aim to encourage the creation and re-use of LOD in archaeology, as we believe it offers an improvement on current data publishing practices.
Taking the Big Leap: A Case Study on Implementing Programmatic Assessment in an Undergraduate Medical Program
Authors: Bonvin Raphaël , Bayha Elke , Gremaud Amélie , Blanc Pierre-Alain , Morand Sabine , Charrière Isabelle , Mancinetti Marco
Published: June 2022

The concept of programmatic assessment (PA) is well described in the literature; however, studies on implementing and operationalizing this systemic assessment approach are lacking. The present case study developed a local instantiation of PA, referred to as Assessment System Fribourg (ASF), which was inspired by an existing program. ASF was utilized for a new competency-based undergraduate Master of Medicine program at the State University of Fribourg. ASF relies on the interplay of four key principles and nine main program elements based on concepts of PA, formative assessment, and evaluative judgment. We started our journey in 2019 with the first cohort of 40 students who graduated in 2022. This paper describes our journey implementing ASF, including the enabling factors and hindrances that we encountered, and reflects on our experience and the path that is still in front of us. This case illustrates one possibility for implementing PA.
Connecting Macroscopic, Molecular, and Symbolic Representations with Immersive Technologies in High School Chemistry: The Case of Redox Reactions
Authors: Wang Lu , Hodges Georgia , Lee Juyeon
Published: June 2022

Redox reaction is a difficult concept to teach and learn in chemistry courses at the secondary level. Although the significance of connecting macroscopic, molecular, and symbolic levels of representation has been emphasized in the chemistry education literature, most redox instruction involves only macroscopic and symbolic representations. To address this challenge, we designed a blended-reality immersive environment (BRE) model, which blends a traditional experiment with immersive technologies to make the molecular representations of redox reactions visible. The effectiveness of this model in supporting students’ learning of redox reactions was first reported in a different article. In this paper, we further explore the features of BRE that drive learning gains. Results from six high school classes in the U.S. with 351 students indicate that integrating the molecular representation through adding the chemical bonds concept facilitates students in making connections between macroscopic and symbolic levels to promote learning. Dynamic demonstrations of electrons’ interaction with particles support students’ understanding of the nature of redox reactions. This study shows the promise of adopting immersive technologies to present all three representations of chemistry concepts in one learning model.
Sprinkle Prebuffer Strategy to Improve Quality of Experience with Less Data Wastage in Short-Form Video Streaming
Authors: Tran Chanh Minh , Nguyen Duc Tho , Bach Nguyen Gia , Tan Phan Xuan , Kamioka Eiji
Published: June 2022

In mobile short-form video streaming, the video application usually provides the user with a playlist of recommended videos to be played one by one. In order to prevent playback stalls caused by possible fluctuations in the mobile network, after finishing buffering the currently-playing video, commercial video players continue to prebuffer (i.e., buffer in advance before playback) one subsequent video in the playlist with as much content as possible. However, since the user can skip a video at any time if he/she does not like it, prebuffering too much video content leads to the wastage of mobile data. Contrarily, without prebuffering any subsequent video, the video player is exposed to high risks of stalling events, which threaten the user’s quality of experience (QoE). In this paper, a novel Sprinkle Prebuffer Strategy (SPS) is proposed to overcome such drawbacks. Once the currently-playing video’s buffer reaches an optimal buffer threshold, the proposed SPS attempts to concurrently prebuffer all subsequent videos in the playlist, each up to an optimal prebuffer threshold. Based on the evaluation results, it is proven that the proposed SPS outperforms the referenced methods in providing the best user’s QoE with reasonable compensation for data wastage.
A Radical Safety Measure for Identifying Environmental Changes Using Machine Learning Algorithms
Authors: Kshirsagar Pravin R. , Manoharan Hariprasath , Selvarajan Shitharth , Althubiti Sara A. , Alenezi Fayadh , Srivastava Gautam , Lin Jerry Chun-Wei
Published: June 2022

Due to air pollution, pollutants that harm humans and other species, as well as the environment and natural resources, can be detected in the atmosphere. In real-world applications, the following impurities that are caused due to smog, nicotine, bacteria, yeast, biogas, and carbon dioxide occur uninterruptedly and give rise to unavoidable pollutants. Weather, transportation, and the combustion of fossil fuels are all factors that contribute to air pollution. Uncontrolled fire in parts of grasslands and unmanaged construction projects are two factors that contribute to air pollution. The challenge of assessing contaminated air is critical. Machine learning algorithms are used to forecast the surroundings if any pollution level exceeds the corresponding limit. As a result, in the proposed method air pollution levels are predicted using a machine learning technique where a computer-aided procedure is employed in the process of developing technological aspects to estimate harmful element levels with 99.99% accuracy. Some of the models used to enhance forecasts are Mean Square Error (MSE), Coefficient of Determination Error (CDE), and R Square Error (RSE).
The Role of Quality Measurements in Enhancing the Usability of Mobile Learning Applications during COVID-19
Authors: Almaiah Mohammed , Hajjej Fahima , Shishakly Rima , Lutfi Abdalwali , Amin Ali , Awad Ali
Published: June 2022

Despite numerous studies offering some evidence about the significance of quality measurements in enhancing the success of m-learning applications, there are still limited studies about the role of quality measurements in promoting the usability of mobile learning systems. Therefore, our study explores the role of quality measurements in promoting the usability of m-learning systems during COVID-19. The results revealed that the service quality, information quality and system quality are the most important factors affecting mobile learning usability among learners during COVID-19. Moreover, these findings are valuable for classifying the significance of these quality elements, which provide guidance on assigning quality aspects to improve this mobile learning usage during COVID-19 in higher education institutions.
A 1-to-3 GHz 5-to-512 Multiplier Adaptive Fast-Locking Self-Biased PLL in 28 nm CMOS
Authors: Wang Binghui , Yang Haigang , Jia Yiping
Published: June 2022

Based on a self-biased architecture, this paper presents a novel adaptive fast-locking, wide operating range and low-jitter phase-locked loop (PLL). A current injection and adaptive bandwidth technology with minimum area overhead is employed to speed up the loop equilibrium acquisition process, without any adverse impact on the steady-state loop dynamics and the jitter performance. The proposed start-up circuit resets the loop to an appropriate initial state in order to shorten the initial ramp-up interval of the voltage-controlled oscillator (VCO), also resulting in cutting down the pull-in time. In addition, a proportional factor is introduced to give some kind of flexibility in the circuit design optimization. The proposed adaptive fast-locking self-biased PLL (AFL-SPLL) is designed and realized in a prototype based on TSMC 28 nm CMOS process, having a supply voltage of 0.9 V and an area of 0.0281 mm2. This PLL demonstrates a tuning range of 1 to 3 GHz and power consumptions from 0.91 mW at 1 GHz to 4.6 mW at 3 GHz operating frequency. The experimental results show that the capture process has been accelerated by up to 84.7% over large division ratios, yet the capture performance did not deteriorate at all for small division ratios. Meanwhile, the circuit implementation gave almost no area increase and yet achieved a reduction in the lock-in time of about 6.5 times, namely from 23.5 μs (without the adaptive locking) to only 3.6 μs (with the adaptive locking) on the maximum operation frequency condition of 3 GHz.
The Use of Excess Electric Charge for Highly Sensitive Protein Detection: Proof of Concept
Authors: Kanashenko Sergey L. , Galiullin Rafael A. , Shumov Ivan D. , Ivanova Irina A. , Ivanov Yuri D. , Kozlov Andrey F. , Ziborov Vadim S. , Ableev Alexander N. , Pleshakova Tatyana O.
Published: June 2022

In highly sensitive bioanalytical systems intended for the detection of protein biomarkers at low and ultra-low concentrations, the efficiency of capturing target biomolecules from the volume of the analyzed sample onto the sensitive surface of the detection system is a crucial factor. Herein, the application of excess electric charge for the enhancement of transport of target biomolecules towards the sensitive surface of a detection system is considered. In our experiments, we demonstrate that an uncompensated electric charge is induced in droplets of protein-free water owing to the separation of charge in a part of the Kelvin dropper either with or without the use of an external electric field. The distribution of an excess electric charge within a protein-free water droplet is calculated. It is proposed that the efficiency of protein capturing onto the sensitive surface correlates with the sign and the amount of charge induced per every single protein biomolecule. The effect described herein can allow one to make the protein capturing controllable, enhancing the protein capturing in the desired (though small) sensitive area of a detector. This can be very useful in novel systems intended for highly sensitive detection of proteins at ultra-low (≤10−15 M) concentrations.
Cooperative Localization Based on Augmented State Belief Propagation for Mobile Agent Networks
Authors: Zhang Bolun , Gao Guangen , Gao Yongxin
Published: June 2022

Belief propagation (BP) is widely used to solve the cooperative localization problem due to its excellent performance and natural distributed structure of implementation. For a mobile agent network, its factor graph inevitably encounters loops. In this case, the BP algorithm becomes iterative and can only provide an approximate marginal probability density function of the estimate with finite iterations. We propose an augmented-state BP algorithm for mobile agent networks to alleviate the effect of loops. By performing state augmentation, the messages in the factor graph will actually be allowed to be backward propagated, which reduces the number of loops in the factor graph, increases the available information of agents, and thus, benefits the localization. Experimental results demonstrate the better performance of the proposed algorithm over the original BP method.
A Forest Fire Recognition Method Using UAV Images Based on Transfer Learning
Authors: Zhang Lin , Wang Mingyang , Fu Yujia , Ding Yunhong
Published: June 2022

Timely detection of forest wildfires is of great significance to the early prevention and control of large-scale forest fires. Unmanned Aerial Vehicle(UAV) with cameras has the characteristics of wide monitoring range and strong flexibility, making it very suitable for early detection of forest fire. However, the visual angle/distance of UAV in the process of image sampling and the limited sample size of UAV labeled images limit the accuracy of forest fire recognition based on UAV images. This paper proposes a FT-ResNet50 model based on transfer learning. The model migrates the ResNet network trained on an ImageNet dataset and its initialization parameters into the target dataset of forest fire identification based on UAV images. Combined with the characteristics of the target data set, Adam and Mish functions are used to fine tune the three convolution blocks of ResNet, and focal loss function and network structure parameters are added to optimize the ResNet network, to extract more effectively deep semantic information from fire images. The experimental results show that compared with baseline models, FT-ResNet50 achieved better accuracy in forest fire identification. The recognition accuracy of the FT-ResNet50 model was 79.48%; 3.87% higher than ResNet50 and 6.22% higher than VGG16.
Measuring Soil Surface Changes after Traffic of Various Wheeled Skidders with Close-Range Photogrammetry
Authors: Ferenčík Michal , Dudáková Zuzana , Kardoš Miroslav , Sivák Miroslav , Merganičová Katarína , Merganič Ján
Published: June 2022

Soil surface is directly affected by heavy traffic of machinery during harvesting operations. Machine traffic often causes damage to forest soil which is visible on the surface (ruts) and invisible changes in, for example, bulk density, penetration resistance, etc. Close-range photogrammetry is the state-of-the-art method used for recording and evaluation of visible changes. This study aims to analyze soil surface changes caused by traffic of three types of wheeled skidders without a load on Cambisol soil in Central Slovakia. We use the Structure-from-Motion (SfM) close-range photogrammetry to record and evaluate depths of ruts and their volumes after 40 passes of individual skidders. We compared Root Mean Square Errors (RMSEs) of dense point clouds created from various numbers of images taken for individual plots. Rut volume changes calculated by the SfM method and from the manual measurements were compared for one skidder. The final values of RMSE did not exceed 10 mm except for the plot with the lowest number of photos. The final rut depths varied between 0.026 and 0.050 m, and their final volume fluctuated from 0.021 to 0.089 m3. The skidder type and the terrain slope had significant impacts on magnitudes of soil changes. The results of the manual and SfM methods assessing soil changes were correlated. Based on the presented results we can conclude that the SfM method can be applied to detect soil surface changes after traffic of forestry machinery.
The Impact of Visual Defects and Neighboring Trees on Wind-Related Tree Failures
Authors: Nelson Michael F. , Klein Ryan W. , Koeser Andrew K. , Landry Shawn M. , Kane Brian
Published: June 2022

Urban trees are often more sun- and wind-exposed than their forest-grown counterparts. These environmental differences can impact how many species grow–impacting trunk taper, crown spread, branch architecture, and other aspects of tree form. Given these differences, windthrow models derived from traditional forest production data sources may not be appropriate for urban forest management. Additionally, visual abnormalities historically labeled as “defects” in timber production, may not have a significant impact on tree failure potential. In this study, we look at urban tree failures associated with Hurricane Irma in Tampa, Florida, USA. We used spatial analysis to determine if patterns of failure existed among our inventoried trees. We also looked at risk assessment data to determine which visual defects were the most common and the most likely to be associated with branch or whole-tree failure. Results indicate that there was no spatial pattern associated with the observed tree failures–trees failed or withstood the storm as individuals. While some defects like decay and dead wood were associated with increased tree failure, other defects such as weak branch unions and poor branch architecture were less problematic.
Tree-Ring Patterns and Growth Response of Abies jaliscana to Climate along Elevational Gradients in the Mountains of Western Jalisco, Mexico
Authors: Guerrero-Hernández Ricardo , Muñiz-Castro Miguel Ángel , Villanueva-Díaz José , Hernández-Vera Gerardo , Vázquez-García José Antonio , Ruiz-Corral José Ariel
Published: June 2022

Jalisco fir (Abies jaliscana) is an endemic tree with restricted distribution, and is considered a Pliocene relict inhabiting the cloud montane forests in the humid-temperate upper zone of western Jalisco Mountains. Despite its ecological importance, to date, there are no studies for this taxon aimed to understand its vulnerability to regional climate variability. We used dendrochronological techniques to investigate tree-ring patterns and the effects of climate variability on the radial growth of Jalisco fir through its elevational range in both southern and northern aspects. We developed tree-ring series that ranged in length from 79 to 152 years. High-elevation trees (2400 m.a.s.l.) showed higher annual radial growth compared to those present in low-elevation sites. Over the last 150 years, Abies jaliscana trees have had higher annual radial growth rates compared to those exhibited by other Abies species. Both winter and early spring precipitation are the main factors determining the growth rates at all the elevation sites for both aspects. In the cold season, mean maximum temperature negatively affected ring-width growth at most of the elevation zones except for two study sites. We conclude that the radial growth of A. jaliscana appears to be smaller during lower rainfall periods in the dry seasons (winter and early spring), and A. jaliscana annual radial growth responds differently to regional climate variability along the elevational gradient and mountain aspects.
Tsunami-Induced Bores Propagating over a Canal, Part II: Numerical Experiments Using the Standard k-ε Turbulence Model
Authors: Elsheikh Nuri , Azimi Amir H. , Nistor Ioan , Mohammadian Abdolmajid
Published: June 2022

This companion paper presents the results of a series of numerical experiments examining the effects of a mitigation canal on the hydrodynamics of a tsunami-like turbulent bore proceeding over a horizontal bed. The hydraulic bores were generated by a dam-break setup which employed impoundment depths of do = 0.20 m, 0.30 m, and 0.40 m. The bore propagated downstream of the impoundments in the flume and interacted with a canal with varying geometry located downstream. The bore then left the flume through a drain located further downstream of the canal. In this study, the effect of the canal depth on the specific momentum and specific energy of hydraulic bores passing over a rectangular canal is numerically studied. The canal width was kept constant, at w = 0.6 m, while the canal depths were varied as follows: d = 0.05 m, 0.10 m, and 0.15 m. The time history of mean flow energy during the bore’s passing over the mitigation canal indicates that the jet stream of the maximum mean flow energy is controlled by the canal depth. The time required to dissipate the jet stream of the maximum vorticity, the turbulent kinetic energy, and the energy dissipation rate all increased as the canal depth decreased. The effect of canal orientation on the bore hydrodynamics was also numerically investigated, and it was found that the impulsive momentum and specific energy reached the highest values for canal orientations of 45 and 60 degrees. For the same canal depth, the highest peak specific momentum occurred with the highest degree of canal orientation (θ = 60°).
The Antifreeze and Cryoprotective Activities of a Novel Antifreeze Peptide from Ctenopharyngodon idella Scales
Authors: Dang Meizhu , Wang Ruifeng , Jia Yangyang , Du Jing , Wang Ping , Xu Yawei , Li Chunmei
Published: June 2022

The purpose of this study is to obtain new antifreeze peptides (AFPs) that are natural, safe, and high activity from Ctenopharyngodon idella scales. The optimal hydrolysis conditions were investigated, and chromatography-based isolation was conducted using thermal hysteresis activity (THA) as an index. Molecular dynamic simulation (MDs) was explored to reveal the antifreeze mechanism of the AFPs. The results showed that the optimal hydrolysis conditions were 4000 U/g papain enzyme for 60 °C at pH 5.0 and substrate concentration (1:10) for 3 h, as unveiled by single-factor experiment results. The AFPs documented a THA of 2.7 °C when the Th was 1.3 °C. Hydrophilic peptide, named GCFSC-AFPs, with a THA of 5.09 °C when the Th was 1.1 °C was obtained after a series isolation of gel filtration, ion exchange, and reversed-phase HPLC chromatography. The AFPs had a molecular weight of 1107.54~1554.72 Da with three main peptides in the amino acid sequence of VGPAGPSGPSGPQ, RGSPGERGESGPAGPSG, and VGPAGPSGPSGPQG, respectively. The survival rate of yeast with GCFSC-AFPs reached 84.4% following one week of exposure at −20 °C. MDs indicated that GCFSC-AFPs interfered with the ice-water interaction and thus inhibited the ice crystallization process. Our data suggested that the GCFSC-AFPs were a novel and potential antifreeze agent in the food industry.
Structure Characterization, Antioxidant and Immunomodulatory Activities of Polysaccharide from Pteridium aquilinum (L.) Kuhn
Authors: Zhao Zhe-Han , Ju Xian-Yan , Wang Kui-Wu , Chen Xin-Juan , Sun Hong-Xiang , Cheng Ke-Jun
Published: June 2022

Pteridium aquilinum (L.) Kuhn (Pteridaceae family) has been widely used as a food and medicine in China and Korea. Previous studies indicate that P. aquilinum contains a variety of bioactive chemical components such as flavonoids, phenols, terpenoids, saponins, polysaccharides, and so on. In the present study, a novel polysaccharide (named as PAP-3) with average molecular weight of 2.14 × 105 Da was obtained from P. aquilinum. The structure was studied through physicochemical and spectroscopic analysis. The results indicated that PAP-3 consists of arabinose, rhamnose, fucose, galactose, mannose, and xylose in a molar ratio of 1.58:1.00:3.26:4.57:4.81:3.33. The polysaccharide is mainly composed of (1→2)-linked xylose and (1→3,6)-linked mannose on the main chain, with (1→2)-linked xylose, (1→6)-linked mannose, and (1→6)- and (1→3,6)-linked galactose as side chains. Galactose, fucose, and xylose are located at the end of the side chains. The in vitro immunomodulatory and antioxidant activities were assayed. PAP-3 has strong free-radical scavenging activity on DPPH and ABTS radicals and significant immunomodulatory activity on RAW264.7 cells. These data provide useful information for further study on the polysaccharides of P. aquilinum and their applications in the food and medical industries.
Phenolic Acid Profiling of Lactarius hatsudake Extracts, Anti-Cancer Function and Its Molecular Mechanisms
Authors: Yang Qiao , Zhang Xiaoyi , Qin Huini , Luo Feijun , Ren Jiali
Published: June 2022

Cancer is still the leading cause of death across the world, and there is a lack of efficient therapies. Lactarius hatsudake is a mushroom with a food and medicine homology that contains numerous biologically active substances. This study aimed to investigate the composition of extracts from Lactarius hatsudake (L. hatsudake) and their anti-cancer function and molecular mechanisms. Our results showed that the total phenolic content of L. hatsudake extracts was 139.46 ± 5.42 mg/g. The following six phenolic compounds were identified from L. hatsudake extracts by HPLC and UPLC-QTOF/MS: gallic acid, pyrogallol, chlorogenic acid, ferulic acid, myricetin, and cinnamic acid. Colorectal cancer cell HCT116 and hepatic cancer cell HepG2 were used to evaluate the anti-cancer function of the L. hatsudake extracts. Compared with HepG2 cells, the L. hatsudake extracts showed stronger anti-cancer activity against HCT116 cells and these were used to study molecular mechanisms. The results indicated that the L. hatsudake extracts could arrest the cancer cell cycle and inhibit cancer cell proliferation, which may be mediated by the MAPK/NFκB/AP-1 signalling pathway; the L. hatsudake extracts also promoted cancer cell apoptosis through a mitochondrial-dependent pathway. Taken together, these findings demonstrate that L. hatsudake ethanol extracts contain six main phenolics and illustrate the remarkable potentiality of L. hatsudake as a source of natural phenolics for cancer prevention and as an adjuvant in the treatment of functional foods.
Response Surface Methodology for Optimization of Buspirone Hydrochloride-Loaded In Situ Gel for Pediatric Anxiety
Authors: Abdallah Marwa H. , Abdelnabi Dina M. , Elghamry Hanaa A.
Published: June 2022

The purpose of the current investigation was to formulate, assess, and optimize oral in situ gels of buspirone hydrochloride (BH) with the specific end goal of expanding the time the medication spends in the stomach, thereby ensuring an extended medication discharge. This would allow the use of a once-a-day dose of liquid BH formulations, which is ideal for the treatment of pediatric anxiety. In situ gels loaded with BH were prepared using various concentrations of sodium alginate (Na alg.), calcium chloride (CaCl2), and hydroxypropyl methylcellulose (HPMC K15M). The in situ gels exhibited the desired consistency, drug distribution, pH, ability to form gel, and prolonged drug release in vitro. The (33) full factorial design was utilized for the revealing of the ideal figures for the selected independent variables, Na alg. (X1), HPMC (X2), and CaCl2 (X3) based on measurements of the viscosity (Y1) and percentage drug release after 6 h (Y2). A pharmacokinetic study of the optimum formulation on rabbits was also performed. The formulation containing 2% of Na alg., 0.9% of HPMC-K15M, and 0.1125% of CaCl2 was selected as the ideal formulation, which gave the theoretical values of 269.2 cP and 44.9% for viscosity and percentage of drug released after 6 h, respectively. The pharmacokinetic study showed that the selected oral Na alg. in situ gel formulation displayed a prolonged release effect compared to BH solution and the marketed tablet (Buspar®), which was confirmed by the low Cmax and high Tmax values. The optimum oral Na alg. in situ gel showed a 1.5-fold increment in bioavailability compared with the drug solution.
Genome-Wide Identification of NRT Gene Family and Expression Analysis of Nitrate Transporters in Response to Salt Stress in Poncirus trifoliata
Authors: Zhao Zeqi , Li Mengdi , Xu Weiwei , Liu Ji-Hong , Li Chunlong
Published: June 2022

The uptake and transportation of nitrate play a crucial role in plant growth and development. These processes mostly depend on nitrate transporters (NRT), which guarantee the supplement of nutrition in the plant. In this study, genes encoding NRT with Major Facilitator Superfamily (MFS) domain were identified in trifoliate orange (Poncirus trifoliata (L.) Raf.). Totally, 56 NRT1s, 6 NRT2s, and 2 NAR2s were explored. The bioinformation analysis, including protein characteristics, conserved domain, motif, phylogenetic relationship, cis-acting element, and synteny correlation, indicated the evolutionary conservation and functional diversity of NRT genes. Additionally, expression profiles of PtrNRTs in different tissues demonstrated that NRT genes possessed spatio-temporal expression specificity. Further, the salt condition was certified to induce the expression of some NRT members, like PtrNPF2.1, PtrNPF7.4, and PtrNAR2.1, proposing the potential role of these NRTs in salt stress response. The identification of NRT genes and the expression pattern analysis in various tissues and salt stress lay a foundation for future research between nitrogen transport and salt resistance in P. trifoliata.
Numerical Modeling and a Parametric Study of Various Mass Flows Based on a Multi-Phase Computational Framework
Authors: Naqvi Mohammad Wasif , KC Diwakar , Hu Liangbo
Published: June 2022

Gravity-driven mass flows are typically large-scale complex multi-phase phenomena involving multiple interacting phases. Various types of mass flows usually exhibit distinct behaviors in their formation, propagation and deposition. In such large-scale geological systems, many uncertainties may arise from the variations in material composition and phase behavior. The present study aims to investigate the important characteristics of some common types of mass flows including debris flows, mudflows and earth flows, based on a recently developed multi-phase computational framework, r.avaflow for flow simulation. Fractions of different phases are varied to reflect different characteristics of material composition of various mass flows and simulate the resulting flow behavior. The evolution of the critical entities during the flow motion, such as velocity, peak discharge, flow height, kinetic energy, run-out distance and deposition is examined; considerable differences among various flows are identified and discussed. Overall, the simulated mudflow cases develop higher velocity, peak discharge, kinetic energy, and longer run-out distance than the debris flow cases. The fluid fraction has a significant influence on the flow dynamics; a higher fluid fraction often leads to higher velocities and long run-out distances, but lower kinetic energy, and it also affects the final deposition and deposition pattern considerably. The present study shows promising potential of a quantitative approach to the physics and mechanics of mass flows that may assist in the risk assessment of such large-scale destructive geological hazards or disasters.
Associations of Health-Related Physical Fitness with Waist Circumference and Abdominal Obesity Risk in Taiwanese Adults
Authors: Chen Yun-Tsung , Lee Po-Fu , Lin Chi-Fang , Su Yan-Jhu , Chen Hui-Ling , Chen Pin-Chun , Lin Hsueh-Yi , Ho Chien-Chang
Published: June 2022

In this study, we aimed to determine the associations among health-related physical fitness measurements, waist circumference (WC), and abdominal obesity risk in Taiwanese adults. We conducted a cross-sectional study based on data from the 2017 Taiwan Scientific Physical Fitness Survey (the TSPFS). We collected the responses from 17,973 participants (7907 men and 10,066 women) aged 23–64 years for this study. The participants completed the study protocol with a standardized structural questionnaire and a series of health-related physical fitness measurements. The fitness measurements included cardiorespiratory fitness (measured by a 3-min progressive knee-up and step test), muscular fitness (measured by a hand grip strength test), and flexibility (measured by a sit-and-reach test). Our anthropometric measurements included height, weight, WC, hip circumference (HC), body mass index (BMI), and waist-to-hip ratio (WHR). We identified the quartiles of the health-related physical fitness results as the dependent variable in the multiple linear and multiple logistic regression analysis to determine the associations of the physical fitness measurements with WC distribution and abdominal obesity risk. We also considered the dose–response relationship. We found that cardiorespiratory fitness, relative grip strength, and flexibility were each significantly negatively associated with WC, but absolute grip strength was significantly positively associated with WC. We also found that higher levels of cardiorespiratory fitness, relative grip strength, and flexibility were each associated with a lower risk of abdominal obesity. Moreover, our secondary finding was of a dose–response relationship between physical fitness performance, WC, and abdominal obesity risk. In short, health-related physical fitness was an effective predictor of waist circumference for both sexes of Taiwanese adults, and higher levels of health-related physical fitness were associated with lower risks of abdominal obesity in Taiwanese adults.
Learning Theory-Driven Tips for Designing Effective Learning Solutions for the Continuous Education of Community Pharmacists to Enhance Patient-Centered Care—A Qualitative Study
Authors: Cerbin-Koczorowska Magdalena , Przymuszała Piotr , Fabianowska Sandra , Gałązka Natalia , Zielińska-Tomczak Łucja
Published: June 2022

The constant development of medical and pharmaceutical sciences and the changing roles of pharmacists highlight the importance of lifelong learning in their profession. Given the identified knowledge gaps in the literature in terms of pharmacists’ preferences for lifelong learning, the study aimed to evaluate the opinions and attitudes of community pharmacists towards lifelong learning, including their previous experiences and educational needs, in order to propose evidence-based tips for designing such solutions and interventions intended for them both in face-to-face and online forms. For this purpose, ten semi-structured in-depth interviews were conducted with Polish community pharmacists on the topic using a thematic guide. Subsequently, they were subjected to literal transcription and interpretative phenomenological analysis by two independent researchers using phenomenology as the qualitative approach. The identified themes covered the topic’s relevance for pharmacists’ work, practice-oriented form and content, previous learners’ experiences as a foundation for further learning, commercial initiatives’ risks, motivation sources, and barriers for participation in lifelong learning solutions so far. Based on the insights provided by the respondents, as well as scientifically proven learning theories and educational principles, ten tips were formulated for designing recipient-friendly learning solutions and interventions within the framework of postgraduate lifelong learning of pharmacists.
Exploring the Role of Serology Testing to Strengthen Vaccination Initiatives and Policies for COVID-19 in Asia Pacific Countries and Territories: A Discussion Paper
Authors: Chotpitayasunondh Tawee , Fisher Dale Andrew , Hsueh Po-Ren , Lee Ping-Ing , Nogales Crespo Katya , Ruxrungtham Kiat
Published: June 2022

This paper provides a comprehensive summary of evidence to explore and position the role of serology testing in the context of coronavirus disease 19 (COVID-19) immunization and policy response in the Asia-Pacific (APAC) region. The document builds on a review of academic literature and existing policies followed by a process of discussion, validation, and feedback by a group of six experts. Six countries and territories—Australia, Hong Kong, India, Indonesia, Thailand, and Taiwan—were sampled to highlight the differing contexts and scenarios in the region. The review includes an overview of (1) the impact of the COVID-19 pandemic, including the emergence of Variants of Concern (VOCs), especially Omicron, (2) the introduction of immunization, (3) the available testing options and potential use of serology testing, (4) the landscape of guidelines and recommendations for their use, and (5) the barriers and challenges to implementing serology testing as a tool to support COVID-19 immunization. Based on the findings, the co-authors propose a set of recommendations to resolve knowledge gaps, to include the use of serology testing as part of the policy response, and to ensure adequate means of implementation. This paper’s target audience includes members of the academic community, medical societies, health providers and practitioners, and decision-makers.
Long-Term Clinical Outcome in Systemic Lupus Erythematosus Patients Followed for More Than 20 Years: The Milan Systemic Lupus Erythematosus Consortium (SMiLE) Cohort
Authors: Gerosa Maria , Beretta Lorenzo , Ramirez Giuseppe Alvise , Bozzolo Enrica , Cornalba Martina , Bellocchi Chiara , Argolini Lorenza Maria , Moroni Luca , Farina Nicola , Segatto Giulia , Dagna Lorenzo , Caporali Roberto
Published: June 2022

Tackling active disease to prevent damage accrual constitutes a major goal in the management of patients with systemic lupus erythematosus (SLE). Patients with early onset disease or in the early phase of the disease course are at increased risk of developing severe manifestations and subsequent damage accrual, while less is known about the course of the disease in the long term. To address this issue, we performed a multicentre retrospective observational study focused on patients living with SLE for at least 20 years and determined their disease status at 15 and 20 years after onset and at their last clinical evaluation. Disease activity was measured through the British Isles Lupus Assessment Group (BILAG) tool and late flares were defined as worsening in one or more BILAG domains after 20 years of disease. Remission was classified according to attainment of lupus low-disease-activity state (LLDAS) criteria or the Definitions Of Remission In SLE (DORIS) parameters. Damage was quantitated through the Systemic Lupus Erythematosus International Collaborating Clinics/American College of Rheumatology damage index (SLICC/ACR-DI). LLAS/DORIS remission prevalence steadily increased over time. In total, 84 patients had a late flare and 88 had late damage accrual. Lack of LLDAS/DORIS remission status at the 20 year timepoint (p = 0.0026 and p = 0.0337, respectively), prednisone dose ≥ 7.5 mg (p = 9.17 × 10−5) or active serology (either dsDNA binding, low complement or both; p = 0.001) were all associated with increased late flare risk. Late flares, in turn, heralded the development of late damage (p = 2.7 × 10−5). These data suggest that patients with longstanding SLE are frequently in remission but still at risk of disease flares and eventual damage accrual, suggesting the need for tailored monitoring and therapeutic approaches aiming at effective immunomodulation besides immunosuppression, at least by means of steroids.
Diagnostic and Prognostic Role of 18F-Fluoroestradiol PET in Metastatic Breast Cancer: The Second Youth of an Older Theranostic Concept
Authors: Fiz Francesco , Bottoni Gianluca , Treglia Giorgio , Trimboli Pierpaolo , Piccardo Arnoldo
Published: June 2022

Since the discovery of the role of female hormones in breast cancer (BC) pathophysiology, in vivo detection of oestrogen receptor (ER) distribution has been one of the major goals of nuclear medicine and molecular imaging [...]
‘One Size Doesn’t Fit for All’: There Is a Need for Targeted Personalized Therapy in Obstructive Sleep Apnea Syndrome
Authors: Pataka Athanasia
Published: June 2022

The estimated prevalence of moderate to severe obstructive sleep apnea syndrome (OSA) has increased by 14–55% over the last few decades [...]
Long-Term Donor Site Morbidity and Flap Perfusion Following Radial versus Ulnar Forearm Free Flap—A Randomized Controlled Prospective Clinical Trial
Authors: Thiem Daniel G. E. , Siegberg Fabia , Römer Paul , Blatt Sebastian , Pabst Andreas , Heimes Diana , Al-Nawas Bilal , Kämmerer Peer W.
Published: June 2022

This clinical prospective randomized controlled study aimed to investigate the differences between Radial (RFFF) and Ulnar (UFFF) Forearm Free Flap in terms of success, performance, and donor site morbidity. Thirty patients with reconstruction of the head and neck region were included. For the first time, this study assessed flap-perfusion characteristics, donor-site-wound-healing dynamics and hand perfusion using hyperspectral imaging. Further, subjective (Likert-scale, DASH-score) and objective (grip/pinch-strength) parameters of donor site morbidity were analysed. Postoperative follow-up was performed until 6 months after index surgery. With 100% of patients, RFFF and UFFF were equally successful. Compared to surrounding reference, UFFF revealed significant lower tissue oxygenation saturation (StO2) than RFFF. Compared with UFFF, blood flow in both the thenar and hypothenar region were significantly reduced 6 months following RFFF transfer. After four weeks, 27% more patients demonstrated impaired wound healing following RFFF transfer. After 6 months, epithelial-surface continuity was restored in all patients of both groups. After 6 months, overall rates of both subjective and objective donor site morbidity were comparable between RFFF and UFFF. RFFF and UFFF both demonstrate similar success rates and HSI-perfusion dynamics following transfer. After 4 weeks, wound-healing disorder appeared significantly more often in RFFF than in UFFF; however, they became equal after 6 months. RFFF and UFFF can be considered as mutual alternatives.
The Influence of Steroid Hormones on Tooth Wear in Children and in Adolescents
Authors: Buchhardt Jeanette , Kiess Wieland , Körner Antje , Biemann Ronald , Hirsch Christian , Child Study Team LIFE Child Study Team
Published: June 2022

(1) Background: From a young age, boys are more often affected by tooth wear than girls. This suggests an influence of the male sex hormone (testosterone) on the aetiology of tooth wear. The aim of the present study was to investigate the incidence of tooth wear in relation to steroid hormone levels in children. (2) Methods: 1022 test persons aged between 10 and 18 (491 male, 531 female) from the LIFE Child study underwent medical and dental examination. Tooth wear was measured through clinical inspection. Blood samples were taken to determine hormone levels (testosterone, SHBG). The level of free testosterone was calculated from the ratio of testosterone to SHBG. Using multivariable methods, the incidence of tooth wear was analyzed as a function of hormone levels, while controlling for confounders such as age, sex, social status, and orthodontic treatment. (3) Results: The incidence of tooth wear increased with age in both sexes. Boys showed significantly more often attrition facets than girls (17.5% vs. 13.2%, p < 0.001). Subjects with tooth wear showed significantly higher free testosterone levels than those without (males: p < 0.001, females: p < 0.05). After controlling for confounding variables, the risk of tooth wear increased by approximately 30.0% with each year of life (odds ratio [OR]boys = 1.29, 95% confidence interval [CI] = 1.04–1.56; [OR]girls = 1.32, 95% CI = 1.08–1.61). In addition, the risk of tooth wear increased by 6.0% per free testosterone scale score only in boys (OR = 1.06, 95% CI = 1.01–1.12). (4) Conclusions: Tooth wear is common in children and in adolescents, and it increases steadily with age in both sexes. The stronger increase and the higher prevalence among male adolescents can be explained by the additional effect of free testosterone.
Intraobserver Assessment of Shear Wave Elastography in Tensor Fasciae Latae and Gluteus Maximus Muscle: The Importance of the Hip Abductor Muscles in Runners Knee Compared to Healthy Controls
Authors: Klauser Andrea S. , de-Koekkoek Felix , Schwabl Christoph , Fink Christian , Friede Miriam , Csapo Robert
Published: June 2022

Background: Iliotibial band syndrome (ITBS) represents one of the most common running related injuries. The pathophysiology is postulated to be caused by excessive ITB tension, impingement and irritation of soft tissues at the lateral femoral epicondyle. However, direct evidence has yet to be found and the multifactorial etiology is under discussion. The purpose was to evaluate stiffness of ITB, gluteus maximus (GM) and tensor fasciae latae (TFL) muscles using shear wave elastography (SWE). Methods: In 14 patients with clinically verified ITBS and 14 healthy controls, three SWE measurements each of ITB, GM and TFL in both legs was performed to determine measurement reliability and between-group and -leg differences. Results: The mean value of ITB was 12.8 m/s with ICC of 0.76, whereas the values measured in the GM were 3.02 m/s with an ICC of 0.87. No statistically significant difference in controls compared to patients were found (p = 0.62). The mean value of TFL was 5.42 m/s in healthy participants, compared to 3.89 m/s patients with an ICC of 0.98 (p = 0.002). Conclusion: Although SWE showed no difference in ITB stiffness, significant differences for TFL muscle stiffness in runner’s knee was found, suggesting that the hip abductor muscles might play a bigger role in the pathophysiology of ITBS. We aimed to implement baseline values for stiffness assessments and prove reliability for further prospective studies of SWE in runner’s knee.