bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficient Access to New Structures with Incorporated 1,2,3-Triazolium Salts as Preferred Dual Cholinesterase Inhibitors
Authors: Mlakić Milena , Ratajec Maja , Ratković Ana , Raspudić Anamarija , Baric Danijela , Šagud Ivana , Lasić Zlata , Odak Ilijana , Škorić Irena
Published: October 2024

In previous research, 1,2,3-triazolium salts showed significant biological activity as potential in-hibitors of the enzymes cholinesterases (ChEs), crucial for neurotransmission. In this research, pairs of uncharged thienobenzo-triazoles and their charged salts were prepared in order to ex-amine further the role of the positive charge on the nitrogen of the triazole ring on the interac-tions in the active site of the enzymes and to compare the selectivity of 1,2,3-triazolium salts in relation to their uncharged analogs obtained by photochemical cyclization. Neutral thienoben-zo-triazoles showed very good selective activity toward butyrylcholinesterase (BChE), while their salts showed excellent non-selective inhibition toward both BChE (the most active 23: IC50 0.47 mikroM) and acetylcholinesterase (AChE) enzymes (the most active 23: IC50 4.4 mikroM). These new structures with incorporated 1,2,3-triazolium salts present the new scaffold for drug development as it is known that the current therapy in Alzheimer's disease (AD) are selective AChE inhibitors, while in Parkinson's and all stages of AD, non-selective inhibitors of ChEs are preferred. Molecular docking of the selected compounds and their corresponding salts into the active sites of ChEs was conducted to identify the interactions responsible for the stability of the non-covalent cholinesterase-ligand complexes. As genotoxicity studies are crucial when devel-oping new active substances and finished drug forms, in silico studies for all the synthesized compounds have shown that compound 18 is the most promising candidate for genotoxic safety.
Biodegradable Stents: A Breakthrough in the Management of Complex Biliary Tract Injuries: A Case Report
Authors: Cicerone Ottavia , Di Gioia Giulio , Pajola Maria , Gallotti Anna , D'Agostino Antonio Mauro , Cionfoli Nicola , Corti Riccardo , Quaretti Pietro , Maestri Marcello
Published: October 2024

Background and Clinical Significance: Biliary tract injuries are a recognized complication of laparoscopic cholecystectomy. Early diagnosis and prompt management are crucial to minimize complications such as bile leaks, strictures, and fistula formation. This case report highlights the use of a biodegradable biliary stent in managing a complex biliary injury and discusses the impact of delayed diagnosis on treatment outcomes. Case Presentation: We present the case of a 30-year-old male who sustained a Strasberg E2 biliary tract injury during a laparoscopic cholecystectomy. Initially misdiagnosed, the injury was only recognized on the fourth postoperative day. The patient underwent a Roux-en-Y hepaticojejunostomy and subsequently developed a postoperative biliary fistula, which was managed with percutaneous drainage. A biodegradable biliary stent was later placed to address a stricture and minimize the need for future interventions. One year later, the patient presented with symptoms of cholangitis, and radiological findings revealed a narrowing of the biliary lumen. The stricture was resolved and an endoscopic gastrojejunal shunt was placed to prevent further complications. The patient is currently in good condition with no signs of further complications. Conclusions: This case emphasizes the importance of early diagnosis in managing biliary tract injuries and highlights the potential of biodegradable stents to reduce the need for repeat interventions. Despite a delayed diagnosis necessitating complex surgical procedures, the use of a biodegradable stent proved effective in managing postoperative complications. Further studies are needed to evaluate the long-term efficacy of biodegradable stents in similar clinical scenarios.
Study on Bioactivie Components of Aromatic Cynanchum thesioides (Freyn) K. Schum by Solvent Fractionation
Authors: Lyu Tao , Kim Woonjung
Published: October 2024

This study evaluated the antioxidant activity of the methanol extract from aromatic Cynanchum thesioides (Freyn) K. Schum. by fractionating it with different solvents, aiming to provide theoretical evidence for the development of products related to aromatic Cynanchum thesioides (Freyn) K. Schum. The yield of the methanol extract was 13.33%, with the water fraction showing the highest yield, followed by n-hexane, n-butanol, dichloromethane, and ethyl acetate. Among these fractions, the ethyl acetate fraction showed the highest antioxidant activity, as indicated by total polyphenol content and ABTS radical scavenging activity. The DPPH radical scavenging activity, reducing power, and SOD-like activity measurements showed no significant difference between the ethyl acetate and n-butanol fractions, as both showed the highest radical scavenging activity. In the FRAP activity measurement, the n-butanol fraction ranked behind the ethyl acetate and dichloromethane fractions in terms of antioxidant activity. Although the ethyl acetate fraction showed the highest antioxidant activity, its yield was only 1.29%, making it unsuitable for product production considering productivity and economy. However, the n-butanol fraction showed overall high antioxidant activity and was approximately 4 times more abundant with a yield of 5.80% compared to the ethyl acetate fraction. Consequently, considering both productivity and economy, the n-butanol fraction is considered suitable for product development and production.
i-PHAOS: An Overview with an Open-Source Collaborative Database on Miniaturized Integrated Spectrometers
Authors: Coppola Carla Maria , De Carlo Martino , De Leonardis Francesco , Passaro Vittorio M. N.
Published: October 2024

On-chip spectrometers are increasingly becoming tools that might help in everyday life needs. The possibility offered by the several available integration technologies and materials to be used to miniaturize spectrometers has led to a plethora of very different devices, that in principle can be compared according to their metrics. Having access to a reference database can help in selecting the most performing on-chip spectrometers and to be up-to-date in terms of standards and developments. In this paper, an overview of the most relevant publications available in literature on miniaturized spectrometers is reported and a database is provided as an open-source project to which researchers can have access and participate in order to improve the share of knowledge in the interested scientific community.
Edible Alginate- Lecithin Films Enriched with Different Coffee Bean Extracts: Formulation, Non-Cytotoxic, Anti-Inflammatory and Anti-Microbial Properties
Authors: Socha Robert , Such Aleksandra , Wisła-Świder Anna , Juszczak Lesław , Nowak Ewelina , Bulski Karol , Frączek Krzysztof , Doskocil Ivo , Lampova Barbora , Koronowicz Aneta
Published: October 2024

The aim of the study was to analyze the functional properties of newly obtained films based on sodium alginate and lecithin with the addition of antioxidant-rich coffee extracts, and to verify their potential as safe edible food packaging materials. In our study we developed alginate-lecithin films enriched with green or roasted coffee bean extracts. The roasting process of coffee beans had a significant impact on the total phenolic content (TPC) in the studied extracts. The highest value of TPC (134.86 mg GAE/L) as well as antioxidant activity (AA) (0.88 mM T/L) was observed for the extract of light roasted coffee beans. Films with addition of medium roasted coffee extracts and baseline films had the highest tensile strength (21.21±0.73 N). The addition of coffee extract improved the barrier properties of films against UV light by a decrease in transmittance values (200-400 nm), regardless of the type of extract added. Studies on Caco-2, HepG2 and BJ cells shown that digestated films were non-cytotoxic materials (100-0,1 ug/mL) and had no negative effect on cell viability, an increase was noted for all cell lines, the highest after 48-hours in dose 1 ug/mL for film with medium-roasted coffee (194.43±38.30) for Caco-2. The tested films at 20% digestate concentrations demonstrated the ability to reduce nitric oxide (NO) production in the RAW264.7 cell line by 25 to 60% compared to the control. Each of the tested films with coffee extracts had growth inhibitory properties towards selected species of bacteria.
Integrating Lean Management and Circular Economy for Sustainable Dentistry
Authors: Antoniadou Maria
Published: October 2024

The increasing focus on environmental sustainability has prompted the healthcare sector, including dentistry, to reconsider waste management practices. Dental offices generate various waste streams from outdated technologies, including single-use plastics, hazardous materials, and digital waste. This scoping review explores how integrating circular economy principles with lean management practices can enhance sustainability and operational efficiency in dental clinics. Using the PRISMA methodology, the review identifies 30 articles that passed 1) the Cochrane Risk of Bias Tool and b) the Critical Appraisal Skills Programme (CASP) checklist from 2000. Circular economy principles focus on reducing waste and maximizing resource reuse, while lean management seeks to improve efficiency and minimize process waste. Together, these approaches can significantly reduce resource consumption and enhance recycling efforts in dental practices. However, barriers include high costs of sustainable technologies, regulatory challenges, and limited recycling infrastructure. The study also highlights case studies of healthcare and dental clinics successfully implementing these strategies, showing how they can overcome challenges and improve environmental and operational outcomes. Collaboration between dental professionals, policymakers, and industry leaders is essential for promoting the adoption of these sustainable practices.
Sharks and Rays of Northern Australia’s Roper River, with a Range Extension for the Threatened Speartooth Shark Glyphis glyphis
Authors: Constance Julia M , Garcia Erica A , Rangers Yugul Mangi , Davies Christy-Louise , Kyne Peter M
Published: October 2024

Northern Australia is considered a ‘lifeboat’ region for globally threatened sharks and rays (elasmobranchs), although much of the region is understudied. The Roper River in the Northern Territory’s Gulf of Carpentaria has been inadequately surveyed, with most elasmobranch data gained opportunistically through freshwater fish surveys. This study aimed to determine the occurrence of elasmobranch species in the Roper River through targeted field surveys conducted between 2016 and 2023 and a review of data from other sources. Four euryhaline species were recorded comprising two sharks, a sawfish, and a stingray. Records of the globally Vulnerable Speartooth Shark (Glyphis glyphis) represented a significant range extension and the documentation of a new breeding population. Records of the Critically Endangered Largetooth Sawfish (Pristis pristis) extended almost 400 km upstream, highlighting extensive use of the river system. A predominantly marine species, the Critically Endangered Giant Guitarfish (Glaucostegus typus) was observed in brackish estuarine waters, approximately 15 km upstream from the river mouth. Further research is required to develop a more thorough understanding of abundance, life history, and population connectivity for these species in this river system. Given the documentation of threatened species, the potential impacts of fisheries, water allocations, and climate change requires assessment.
Development and Evaluation of ABI-171, a New Fluoro Catechin Derivative, for the Treatment of Idiopathic Pulmonary Fibrosis (IPF)
Authors: Araldi Gian Luca , Hwang YuWen , Raghu Ganesh
Published: October 2024

The persistent challenge of IPF, characterized by disease progression and high mortality, underscores the urgent need for innovative therapeutic strategies. We have developed a novel small molecule – catechin derivative ABI-171 – selectively targeting DYRK1A and PIM1 kinases, crucial in the pathogenesis of fibrotic processes. We employed the Bleomycin-induced (intratracheal) mouse model of pulmonary fibrosis (PF) to evaluate the therapeutic efficacy of ABI-171. Mice with induced PF were treated QD with ABI-171 either prophylactically or therapeutically using oral and intranasal routes. Pirfenidone (100mg/kg, TID) and Epigallocatechin gallate (EGCG, 100 mg/kg, QD), a natural catechin currently in a Phase 1 clinical trial, were used as reference compounds. ABI-171, administered prophylactically, led to a significant reduction in hydroxyproline levels and fibrotic tissue formation compared to the control group. Treatment with ABI-171 improved body weight, indicating mitigation of disease-related weight loss. Additionally, ABI-171 demonstrated anti-inflammatory activity, reducing lymphocyte and neutrophil infiltration. In the therapeutic setting, ABI-171, administered 7 days post-induction, reduced mortality rates (P = 0.04) compared with the bleomycin and EGCG control groups. ABI-171 also ameliorated the severity of lung injuries assessed by improved Masson’s trichrome scores when administered both orally and intranasally. ABI-171 significantly decreases bleomycin-induced PF and improves survival in mice, showcasing promising therapeutic potential beyond current medications like Pirfenidone and EGCG for patients with IPF. Based on these results, further studies with ABI-171 are ongoing in preclinical studies.
An Oxford Nanopore Technology-Based Hepatitis B Virus Sequencing Protocol Suitable For Genomic Surveillance Within Clinical Diagnostic Settings
Authors: Tshiabuila Derek , Choga Wonderfull , San James E. , Maponga Tongai G , Preiser Wolfgang , Van Zyl Gert U. , Moir Monika , Van Wyk Stephanie , Giandhari Jennifer , Pillay Sureshnee , Anyaneji Ugochukwu Jacob , Lessells Richard John , Naidoo Yeshnee , Sanko Tomasz , Wilkinson Eduan , Tegally Houriiyah , Baxter Cheryl , Martin Darren P. , de Oliveira Tulio
Published: October 2024

Chronic Hepatitis B Virus (HBV) infection remains a significant public health concern, particularly in Africa, where the burden is substantial. HBV is an enveloped virus, classified into ten phylogenetically distinct genotypes (A – J). Tests to determine HBV genotypes are based on full-genome sequencing or reverse hybridization. In practice, both approaches have limitations. Whereas diagnostic sequencing, generally using the Sanger approach, tends to focus only on the S-gene and yields little or no information on intra-patient HBV genetic diversity, reverse hybridization detects only known genotype-specific mutations. To resolve these limitations, we developed an Oxford Nanopore Technology (ONT)-based HBV diagnostic sequencing protocol suitable for clinical virology that yields both complete genome sequences and extensive intra-patient HBV diversity data. Specifically, the protocol involves tiling-based PCR amplification of HBV sequences, library preparation using the ONT Rapid Barcoding Kit, ONT GridION sequencing, genotyping using Genome Detective software, recombination analysis using jpHMM and RDP5 software, and drug resistance profiling using Geno2pheno software. We prove the utility of our protocol by efficiently generating and characterizing high-quality near full-length HBV genomes from 148 residual diagnostic samples from HBV-infected patients in the Western Cape province of South Africa, providing valuable insights into the genetic diversity and epidemiology of HBV in this region of the world.
An Oxford Nanopore Technology-Based Hepatitis B Virus Sequencing Protocol Suitable For Genomic Surveillance Within Clinical Diagnostic Settings
Authors: Tshiabuila Derek , Choga Wonderfull , San James E. , Maponga Tongai G , Van Wyk Stephanie , Giandhari Jennifer , Pillay Sureshnee , Anyaneji Ugochukwu Jacob , Naidoo Yeshnee , Baxter Cheryl , Martin Darren P. , de Oliveira Tulio
Published: October 2024

Chronic Hepatitis B Virus (HBV) infection remains a significant public health concern, particularly in Africa, where the burden is substantial. HBV is an enveloped virus, classified into ten phylogenetically distinct genotypes (A – J). Tests to determine HBV genotypes are based on full-genome sequencing or reverse hybridization. In practice, both approaches have limitations. Whereas diagnostic sequencing, generally using the Sanger approach, tends to focus only on the S-gene and yields little or no information on intra-patient HBV genetic diversity, reverse hybridization detects only known genotype-specific mutations. To resolve these limitations, we developed an Oxford Nanopore Technology (ONT)-based HBV diagnostic sequencing protocol suitable for clinical virology that yields both complete genome sequences and extensive intra-patient HBV diversity data. Specifically, the protocol involves tiling-based PCR amplification of HBV sequences, library preparation using the ONT Rapid Barcoding Kit, ONT GridION sequencing, genotyping using Genome Detective software, recombination analysis using jpHMM and RDP5 software, and drug resistance profiling using Geno2pheno software. We prove the utility of our protocol by efficiently generating and characterizing high-quality near full-length HBV genomes from 148 residual diagnostic samples from HBV-infected patients in the Western Cape province of South Africa, providing valuable insights into the genetic diversity and epidemiology of HBV in this region of the world.
High-Energy Neutrino Flavor State Transition Probabilities
Authors: Harrison John , Anantua Richard
Published: October 2024

We analytically determine neutrino transitional probabilities and abundance ratios at various distances from the source of creation in several astrophysical contexts, including the Sun, supernovae and cosmic rays. In doing so, we determine the probability of a higher-order transition state from $\nu_\tau\rightarrow\nu_\lambda$, where $\nu_\lambda$ represents a more massive generation than Standard Model neutrinos. We first calculate an approximate cross-section for high-energy neutrinos which allows us to formulate comparisons for the oscillation distances of solar, supernova and higher-energy cosmic ray neutrinos. The flavor distributions of the resulting neutrino populations from each source detected at Earth are then compared via fractional density charts.
Design, Control, and Testing of a Multifunctional Soft Robotic Gripper
Authors: Correia Ana , Charters Tiago , Leite Afonso , Campos Francisco , Monge Nuno , Rocha André , Mendes Mário J.G.C.
Published: October 2024

This paper proposes a multifunctional soft robotic gripper for a Dobot robot to handle sensitive products. The gripper is based on pneumatic network (PneuNet) bending actuators. In this work, two different models of PneuNet actuators have been studied, designed, simulated, experimentally tested, and validated using two different techniques (3D printing and molding) and three different materials: FilaFlex (3D printed), Elastosil M4601 and Dragonskin Fast 10 silicones (with molds). A new soft gripper design for the Dobot robot is presented, and a new design/production approach with molds is proposed to obtain the gripper's PneuNet multifunctional actuators. It also describes a new control approach that is used to control the PneuNet actuators and gripper function, using compressed air generated by a small compressor/air pump, a pressure sensor, a mini valve, etc., and executing on a low-cost controller board – Arduino UNO. This paper presents the main simulation and experimental results of this research work.
Immediate Effects of distinct intensities of Transcutaneous Spinal Direct Current Stimulation on Chronic Pain: A Randomized Controlled Trial
Authors: Ferreira Kariny Realino do Rosário , Macedo Maria de Cássia Souza , Alves Ana Luiza Guimarães , Esquírio Arthur Ferreira , Botim Bianca Rossi , Jacob Gabrielly Souza , dos Santos Mayra Evelise Cunha , Gama Gabriela Lopes , Barbosa Michelle Cristina Sales Almeida , Barbosa Alexandre Wesley Carvalho
Published: October 2024

This study aimed to assess the immediate effects of transcutaneous spinal direct current stimulation (tsDCS) on pain outcomes, measured by the visual analog scale (VAS) and pressure pain thresholds in a cohort of 55 participants experiencing chronic pain. A controlled, randomized trial with 55 participants allocated into 2 groups: 2 mA and 0.5 mA of tsDCS for 20 minutes. Anodal stimulation was applied on the 12th thoracic vertebra, and cathodal stimulation on the 7th cervical vertebra. Pain outcomes were assessed before and post-intervention using the VAS and pressure algometry. Between and within-group differences, along with chi-square tests were used to determine the differences and responsiveness. Significance was established at p < 0.05. Findings showed significant temporal effect for both VAS (p < 0.001) and pressure algometry (p = 0.04). However, no between-group differences were noted in the time*group factor for VAS (p = 0.466) and pressure algometry (p = 0.78). No significant between-group differences were observed for the responsiveness analysis. The results indicate that a single 20-minute session of 2 and 0.5 mA tsDCS improves pain scores for both intensities equally. However, there were no statistically significant between-group differences in pain perception or pressure pain threshold.
Medical Ozone. Redox Regulation and a New Mechanism in the Treatment of Rheumatoid Arthritis
Authors: León Fernández Olga Sonia , Oru Gabriel Takon , Viebahn-Haensler Renate , López Cabreja Gilberto , Serrano Espinosa Irainis , Corrales Vázquez María Elena
Published: October 2024

Medical ozone is a redox regulator with beneficial effects in oxidative etiology diseases such as rheumatoid arthritis (RA). The aim of this study was to conduct a holistic review from different pharmacological trials involving ozone in model diseases as well as the clinical response of RA patients. The ROS involved in RA and its relationship to the main pathological pathways of this autoimmune disease were here considered. The integrator analysis of experimental results from animals with clinical findings have revealed that both methotrexate (MTX) and medical ozone share common mechanisms via adenosinergic regulation. This finding has enabled us to propose a new pharmacological mechanism in the treatment of RA. We concluded that MTX + medical ozone combined therapy reduces ROS overproduction and the generation of proinflammatory cytokines, and decreases anti-cyclic citrullinate peptide levels by a mutual mechanism involving adenosine A1 receptors.
Dynamic Risks in Automotive Factories: Fire Risk in Lead-Acid Battery Manufacturing Process – 3D Simulations and Case Study
Authors: Zinveli Ankidim , Dragomir Mihai , Dragomir Diana
Published: October 2024

The present paper addresses dynamic risks in the automotive industry factories, specifically the car lead-acid batteries manufacturing area. The main analyzed risk is represented by the fire risk. Battery manufacturing process is described and analyzed from the fire risk perspective and the hazard areas are identified. The investigation methodology uses case studies for different lead-acid battery formation processes, combined with 3D simulations using PyroSym platform, and it is based on the author's experience in the battery manufacturing field for about ten years. The results of the case studies are compared using the same inputs, but different specific process conditions, and conclusions are formulated. To avoid the fires and mitigate the risk, a series of actions are proposed in the last sections. As a general conclusion, the actual research demonstrates that the complex and dynamic risks from the automotive industry, associated with Industry 5.0 must be analyzed using combined methods, classical and modern, including 3D simulations, to get great results.
Early-Stage IM Treatment with the Host-Derived Immunostimu-Lant CPDI-02 Increases Curative Protection of Healthy Outbred Mice Against Subcutaneous Infection with CA-MRSA USA300
Authors: Vetro Joseph
Published: October 2024

Background/Objectives: Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) greatly complicates treatment of skin and soft tissue infections (SSTI). It was previ-ously found that subcutaneous (SQ) treatment with the mononuclear phagocyte (MP)-selective activator, complement peptide-derived immunostimulant-02 (CPDI-02; formerly EP67), increases prophylaxis of outbred CD-1 mice against SQ infection with CA-MRSA. Here, we determined if treatment with CPDI-02 also increases curative protection. Methods: Female CD-1 mice were challenged SQ with CA-MRSA USA300 LAC then CPDI-02 or inactive scCPDI-02 was administered by a topical, SQ, IM, or IV route at 6- or 24-hrs post-challenge. Dermal abscess sizes were compared over 10 days and CA-MRSA burden, neutrophils, MP, and pro-inflammatory cytokines were compared in dermal abscesses. CPDI-02 PK and distribution in female CD-1 mice were compared after IM or IV dosing and CPDI-02 toxicity was determined by IM dose escalation and repeat IM dosing in male and female CD-1 mice. Results: Repeat IM treatment with CPDI-02 starting at 6-hrs post-challenge decreased maximum abscess surface area, CA-MRSA burden, and time to resolu-tion, whereas repeat treatment by the topical, SQ, or IV route had no effect. Starting CPDI-02 treatment at 24-hrs post-challenge was ineffective regardless of route. Single IM treatment with CPDI-02 starting at 6-hrs post-challenge was as effective as repeat IM treatment, increased systemic exposure, and decreased initial levels of IL-1β and increased MP in dermal abscesses. CPDI-02 was tolerated to between 130-170 mg/kg for single IM dosing and 65-130 mg/kg for repeat IM dosing with males being more tolerant. Conclusions: Single early-stage IM treatment with CPDI-02 may increase curative protection against SSTI caused by CA-MRSA or other pathogens controlled by activated MP.
Genome-Wide Characterization and Transcriptional Analyses of the Calmodulin and Calmodulin-Like Protein Family in Canavalia rosea Provide Insight into Their Potential Roles in Abiotic Stress Tolerance
Authors: Ding Qianqian , Huang Zengwang , Wang Zhengfeng , Jian Shuguang , Zhang Mei
Published: October 2024

Calmodulins (CaMs) and calmodulin-like-proteins (CMLs) belong to families of calcium-sensors that act as calcium-ion (Ca2+) signal-decoding proteins and regulate downstream target proteins. As a tropical halophyte, Canavalia rosea shows great resistance to multiple abiotic stresses, including high salinity-alkaline, extreme drought, heat, and intense sunlight. However, investigations of calcium ion signal transduction involved in the stress responses of C. rosea are limited. The CaM and CML gene families have been identified and characterized in many species. Nevertheless, there is limited available information about these genes in C. rosea. In this study, a bioinformatic analyses, including the gene structures, conserved domains, phylogenetic relationships, chromosome distribution, and gene synteny, was comprehensively performed to identify and characterize the CrCaM and CrCML gene members. A spatio-temporal expression analysis in different organs and environmental conditions were then assayed using the RNA sequencing technique. Additionally, several CrCaM and CrCML members were then cloned and functionally characterized using the yeast heterogeneous expression system, and some of them were found to change the tolerance of yeast to heat, salt, alkaline, and high osmotic stresses. Furthermore, this indicated that the CrCaMs and CrCMLs play important roles in stress resistance. The results of this study provide a foundation for understanding the possible roles of plant CaM and CML genes, especially for halophyte C. rosea natural ecological adaptability. This study also provides a theoretical basis for further study of the physiological functions of plant CrCaMs and CrCMLs that are involved in multiple abiotic stresses.
Soil Sensor Use in Delimiting Management Zones for Sowing Maize in No-Till
Authors: Bottega Eduardo Leonel , Pinto Ederson Bitencourt , Saretta Ezequiel , Oliveira Zanandra Boff de , Severo Filipe Silveira , Assmann Johan
Published: October 2024

This study aimed to analyze the productive behavior of the maize crop, cultivated at different population densities, in management zones (MZ) delimited based on mapping the spatial variability of the soil apparent electrical conductivity (ECa). The soil ECa was measured and two MZs were subsequently delimited, one with low and the other with high ECa. In each MZ, four maize sowing densities were tested: 60,000 (D1); 80,000 (D2); 100,000 (D3) and 140,000 (D4) seeds ha-1. Ear length, number of grains per ear, number of grains per row, number of rows per ear, thousand-grain weight, and yield were evaluated. The increase in sowing density in the high ECa MZ linearly reduced the values of ear diameter, number of rows per ear, number of grains per ear and thousand-grain weight. Sowing density D3, when implemented in the low ECa MZ, showed higher values for the ear length, ear diameter, number of grains per row, number of grains per ear, and thousand-grain weight. Sowing density D2 was the one with the highest yield, regardless of the MZ where it was implemented (5,628.48 kg ha-1 in the high ECa management zone and 4,463.63 kg ha-1 in the low ECa).
COVIDALISM®©: COVID Restrictions in USA Have No Health Benefits at All
Authors: BIRO JAN
Published: October 2024

Background: Short after the detection of the COVID virus in January 2020 the US Government introduced and enforced a series of restrictions to protect the elderly from a “deadly virus” and the “pandemic of the century”. Persons who disagreed were silenced and punished. Objective: In the 15th month of COVID epidemic (April 2021), we have access to sufficient statistical data and methods to better understand the nature, origin of the COVID pandemic. It is now possible to reliably evaluate the effectiveness of the restrictions and the human factors / driving forces behind this drastic limitations of our natural freedom in this country. Methods: Publicly available epidemiological and population parameters were collected and analyzed using the ‘political score’ and ‘restriction ranking’ of 50 states and evaluated by simple and usual statistical methods, mainly correlation analyses. Results: This study revealed, that 1) restrictions reduced the number of viral infections, but 2) they totally failed to reduce the number of supposedly COVID related deaths, 3) they increased (SIC!) the mortality of coronavirus. The grade of restrictions were/are strongly associated to the 4) left/right political ratio of the States, there States with more democrat citizens practiced more restrictions. It was found that race, religion and Medicare spending have significant influence on politic as well as on the grade of restriction orders. Factors moving States toward the political left and harder COVID restrictions have 5) larger ‘non-white’ population, 6) larger number of ‘non-protestant’ believers, 7) larger Jewish population, and 8) more generous Medicare spending. It was not possible to see any influence of the size of the 9) senior (65+) population [i.e. those who are allegedly the most vulnerable and are mostly in need of protective restrictions] neither on the States politic nor on the restrictions Conclusions: The conclusion is that COVID restrictions are primarily politically motivated, they are ineffective to reduce COVID related mortality and they provide no extra health benefit for the so called “vulnerable” minority (elderly).
Gradual Loss of UV Tolerance with Increasing Habitat Depths in Deep-Freshwater Amphipods (Amphipoda, Crustacea) of Ancient Lake Baikal
Authors: Kondrateva Elizaveta , Gurkov Anton , Rzhechitskiy Yaroslav , Saranchina Alexandra , Diagileva Anastasiia , Drozdova Polina , Vereshchagina Kseniya , Shatilina Zhanna , Sokolova Inna , Timofeyev Maxim
Published: October 2024

Solar ultraviolet (UV) is among the most important ecological factors shaping the composition of biota on the planet surface, including upper layers of waterbodies. Inhabitants of dark environments recently descending from surface organisms provide natural opportunities to study the evolutionary losses of UV adaptation mechanisms and better understand how those mechanisms function at the biochemical level. Ancient Lake Baikal is the only freshwater reservoir, where deep-water fauna emerged, and its diverse endemic amphipods (Amphipoda, Crustacea) now inhabit the whole range from highly transparent littoral to dark depths of over 1600 m, which makes them a convenient model to study UV adaptation. Here we show that adults of deep-water Baikal amphipods Ommatogammarus flavus and O. albinus indeed have high sensitivity to environmentally relevant UV levels in contrast to littoral species Eulimnogammarus cyaneus and E. verrucosus. The UV intolerance was more pronounced in deeper-dwelling O. albinus and was explainable by lower levels of carotenoids and carotenoid-binding proteins. Signs of oxidative stress were not found but specifically UV-B seemingly led to accumulation of toxic compounds. Overall, the obtained results demonstrate that UV is an important factor limiting distribution of deep-water amphipods into the littoral zone of Lake Baikal.
Screening for Hypophosphatasia in Adult Patients at a Maximum Care Provider – Retrospective Analyses over Fifteen Years
Authors: Hennings Robert , LeDuc Gabriela-Diana , Tönjes Anke , Lemke Johannes , Thiery Joachim , Kratzsch Jürgen , Roth Andreas
Published: October 2024

Introduction: Persistent hypophosphatasia (pHPE) can be an expression of hypophosphatasia (HPP), a rare disorder of bone metabolism resulting from ALPL gene pathogenic variants. The aim of this study was to identify the role of auxiliary general biomarkers to verify low ALP serum activity as an alert parameter for an ALPL gene pathogenic variants.
Material: A retrospective analysis of adult patients with at least one reduced ALP value of less than 21 U/L was performed. 88 patients had a temporary HPE (tHPE group). 12 out of 20 patients with persistent HPE agreed to a genetic analysis. 11 patients had a pathogenic ALPL gene variant (ALPL group). Hemoglobin [HB], alanine aminotransferase [ALT], aspartate aminotransferase [AST], gamma-glutamyl transferase [GGT], calcium, phosphate, thyrotropin [TSH], albumin, total protein, and C-reactive protein [CRP] levels) represented basic biomarkers. A statistical comparison of general biomarkers was performed between both groups.
Results: The mean HB, ALP, AST, calcium, albumin, and total protein levels were lower in the tHPE group (p
Interactomic Analyses and a Reverse Engineering Study Identify Specific Functional Activities of One-to-One Interactions of the S1 Subunit of the SARS-CoV-2 Spike Protein with the Human Proteome
Authors: Colonna Giovanni ---
Published: October 2024

The S1 subunit of SARS-CoV-2 Spike has been found in the blood of covid patients and vaccinated individuals. From BioGRID, we selected 146 significant human proteins experimentally interacting with S1. Then, we derived an interactome model that facilitated the study of functional activities. Through a reverse engineering approach, we identified 27 specific one-to-one interac-tions of S1 with the human proteome. S1 interacts in this manner independently from the biological context in which it operates, be it infection or vaccination. Instead, when it works together with viral proteins, carry multiple attacks on single human proteins out, showing a different functional engagement. Through Cytoscape we showed functional implications and its tropism to human organs/tissues, such as nervous system, liver, blood, and lungs. As a single protein, S1 operates in a complex metabolic landscape which includes 2557 GO biological processes, much more than the 1430 terms controlled when operating in a group. A Data-Merging approach shows that the total proteins involved by S1 in the cell are over 60,000 with an average involvement per single bio-logical process of 26.19. However, many human proteins get entangled in over 100 biological different activities each. Clustering analysis showed statistically significant activations of many molecular mechanisms, like those related to hepatitis-B infections. This suggests potential in-volvement in carcinogenesis, based on a viral strategy that uses the ubiquitin system to impair the tumor suppressor and antiviral functions of TP53, as well as the role of RPS27A in protein turnover and cellular stress responses.
Physical, Chemical, Mineralogical, and Toxicological Characterization to Identify Heavy Metals Hg, As, Pb, CD, and Zn in Active and Inactive Tailings in the Arequipa Region, Peru
Authors: Palma Gregorio , Castillo Dery , Palma Karol , Santander Lizbeth , Bolaños Héctor , Navarro Patricio
Published: October 2024

The mining industry is a fundamental pillar of economic development in Peru, but mine tailings can generate contamination by toxic heavy metals such as Hg, As, Pb, Cd, and Zn, affecting health, flora, and fauna. This research aimed to characterize the physical, chemical, mineralogical, and toxicological properties of active and passive tailings in the Arequipa region of Peru to identify and quantify the presence of these heavy metals. Analytical techniques, such as granulometry, ICP, TCLP, SEM, and EDS were used to obtain detailed information about the characteristics of the tailings. The results showed significant variability in particle size distribution, as well as differences in mineralogical composition, including the presence of valuable and potentially toxic minerals. This study will allow corrective actions to be taken to prevent, minimize, or remediate potential negative impacts in the region.
Graph Neural Network Based Learning for Facility Location Optimization
Authors: Cvancara Carmen
Published: October 2024

This paper investigates the application of Graph Neural Networks (GNNs) in solving the facility location decision problem. By adapting the learning objectives and structure of GNNs, this research bridges the gap between traditional optimization approaches and modern machine learning techniques. The proposed method demonstrates improved decision-making capabilities, providing promising results for facility location optimization. Additionally, potential future research directions are outlined, highlighting areas where GNNs can further enhance decision-making processes in complex supply chain networks. Hybrid GNN and Mixed Integer Programming solutions have been proposed.
THOP1 Is Entailed in a Genetic Fingerprint Associated with Late-Onset Alzheimer’s Disease
Authors: Climer Sharlee
Published: October 2024

In a systematic explorative study of genetic patterns on chromosome 19, we discovered a pattern comprised of 23 SNP alleles that is significantly associated with late-onset Alzheimer’s disease (AD). This association is validated using two independent data sets. The pattern includes THOP1, which has a long and disputatious relationship with AD. It also spans SLC39A3 and SGTA and is upstream from DIRAS1. We utilized population data to observe the frequencies of this genetic pattern for 11 different ancestries and note that it is highly common for Europeans and relatively infrequent for Africans. This research provides a distinct genetic signature for AD risk as well as insights into the complicated relationship between this disease and THOP1.
Acute and Chronic Resistance Training, Acute Endurance Exercise, nor physiologically Plausible Lactate In Vitro Affect Skeletal Muscle Lactylation
Authors: Mattingly Madison L. , Anglin Derick A. , Ruple Bradley A. , Scarpelli Maira C. , Bergamasco Joao G. , Godwin Joshua S. , Mobley C. Brooks , Frugé Andrew Dandridge , Libardi Cleiton A. , Roberts Michael D.
Published: October 2024

We examined changes in skeletal muscle protein lactylation and acetylation in response to acute resistance exercise, chronic resistance training (RT), and a single endurance cycling bout. Additionally, we performed in vitro experiments to determine if different sodium lactate treatments affect myotube protein lactylation and acetylation. The acute and chronic RT study (n=12 college-aged participants) consisted of 10 weeks of unilateral leg extensor RT with vastus lateralis (VL) biopsies taken at baseline, 24-hours following the first RT bout, and the morning of the last day of the RT bout. For the acute cycling study (n=9 college-aged participants), VL biopsies were obtained before, 2 hours, and 8 hours after 60 minutes of cycling. For in vitro experiments, C2C12 myotubes were treated with varying levels of sodium lactate including LOW (1mM for 24-hours), HIGH (10mM for 24 hours), and PULSE (10mM for 30 minutes followed by 1 mM for 23.5-hours). Neither acute nor chronic RT significantly affected nuclear or cytoplasmic protein lactylation. However, cytoplasmic protein acetylation was significantly reduced following one RT bout (-15%, p=0.002) and chronic RT (-16%, p=0.006). Cycling did not acutely alter post-exercise global protein lactylation or acetylation patterns. Lastly, varying 24-hour lactate treatments did not alter nuclear or cytoplasmic protein lactylation or acetylation, cytoplasmic protein synthesis levels, or myotube diameters. These findings continue to support that exercise induces changes in skeletal muscle protein acetylation but not lactylation.
Blockchain and Internet of Things Technologies for Food Traceability in Olive Oil Supply Chains
Authors: Vitaskos Vassilios , Demestichas Konstantinos , Karetsos Sotirios , Costopoulou Constantina
Published: October 2024

This paper explores the application of Blockchain technology in food traceability, focusing specifically on olive oil. It addresses the significant challenges in the food industry, such as verifying authenticity and detecting harmful substances. The research highlights the potential of Blockchain to enhance traceability by leveraging its decentralized and secure architecture, which en-sures data integrity and facilitates rapid monitoring throughout the supply chain. The paper also examines the synergy between Blockchain and the Internet of Things (IoT), which is critical for real-time tracking in product production. The research conducted has led to a practical demonstration through a web application connected to the Ethereum test network, designed for the quality control of olive oil via smart contracts. Overall, this study provides a comprehensive analysis of Blockchain technology and its transformative potential in ensuring food traceability, particularly for high-value commodities like olive oil.
Assessing the Utility of ColabFold and AlphaMissense in Determining Missense Variant Pathogenicity for Congenital Myasthenic Syndromes
Authors: Ryan-Phillips Finlay , Dong Yin Yao
Published: October 2024

Background/Objectives: Congenital Myasthenic Syndromes (CMS) are caused by variants in >30 genes with increasing numbers of variants of unknown significance (VUS) discovered by next generation sequencing. Establishing VUS pathogenicity requires in vitro studies that slow diagno-sis and treatment initiation. Recent protein structure prediction softwares, AlphaFold2/ColabFold, have revolutionised structural biology; such predictions have also been leveraged in AlphaM-issense which predicts ClinVar variant pathogenicity with 90% accuracy. Few reports, however, have tested these tools on rigorously-characterised clinical data. We therefore assessed ColabFold and AlphaMissense as diagnostic aids for CMS, using variants of the CHRN genes that encode the nicotinic acetylcholine receptor (nAChR).
Methods: Utilising a dataset of 61 clinically-validated CHRN variants, (1) we evaluated the pos-sibility of a ColabFold metric (either predicted structural disruption, prediction confidence, and prediction quality), that distinguishes variant pathogenicity. (2) We assessed AlphaMissenses’ ability to differentiate variant pathogenicity. (3) We compared AlphaMissense to existing patho-genicity prediction programmes, AlamutVP and EVE.
Results: Analysing variant effects on ColabFold CHRN structure prediction, prediction confi-dence, and prediction quality, doesn’t yield any pathogenicity-indicative metric. However, Al-phaMissense predicts variant pathogenicity with 63.93% accuracy on our dataset – a much greater proportion than AlamutVP (27.87%) and EVE (28.33%).
Conclusions: Emerging in silico tools can revolutionise genetic disease diagnosis – however, im-provement, refinement, and clinical validation is imperative prior to practical acquisition.
Spatio-Temporal Analysis of Urban Heat Island (UHI) Intensity in Mbombela City, Mpumalanga Province from 2008 to 2023
Authors: Magagula Njabulo Quentin
Published: October 2024

Urban Heat Islands (UHI) are a growing concern in rapidly urbanizing regions, leading
to significant ecological and public health challenges. This study investigates the spatiotemporal dynamics of UHI intensity in Mbombela City, Mpumalanga Province, South
Africa, from 2008 to 2023. Using Landsat satellite imagery and geospatial analysis
techniques, the research quantifies changes in land cover and their impact on Land
Surface Temperature (LST) and UHI intensity. The results show that built-up areas
increased by 86.67% over the study period, while vegetation cover decreased by
22.22%. Mean LST rose from 28.3°C in 2008 to 30.1°C in 2023, indicating a significant
intensification of the UHI effect. Statistical analyses reveal strong correlations between
urbanization metrics and UHI intensity, highlighting the role of human activities in
exacerbating this phenomenon. This research contributes valuable insights for urban
planning and climate adaptation strategies, particularly in secondary cities like
Mbombela, where rapid development is creating new environmental challenges. The
findings underscore the need for green infrastructure and nature-based solutions to
mitigate the negative impacts of urbanization.
Households Willingness to Use Water from a Solar Water Disinfection Treatment System for Household Purposes
Authors: Afitiri Abdul-Rahaman , Afrifa Ernest Kofi Amankwa
Published: October 2024

The consumption of contaminated water contributes to the global burden of diarrhea and other water-borne diseases, especially among young children. While the decentralized solar water disinfection treatment system remains a viable option to have safe drinking water, our understanding of the effects of household water treatment before use on willingness to accept and adopt a decentralized solar water disinfection treatment system is limited. In this study, a complementary log-log regression analysis of the compositional and contextual factors that systematically vary with willingness to accept and adopt a decentralized solar water disinfection treatment system in the Sawla-Tuna-Kalba (STK) district of Ghana was carried out. Based on our findings, a greater proportion of households’ (97%) are willing to accept and adopt a decentralized solar water disinfection treatment system. Compositional and contextual factors such as age, marital status, education, religion, and geographical location significantly contribute to households’ willingness to accept and adopt a decentralized solar water disinfection treatment system in the STK district of Ghana. Households that treat their water before use (66%, P<0.05) are more likely to accept and adopt a solar water disinfection treatment system compared to those that do not treat their water before use. The findings therefore suggest that scaling up solar water disinfection systems in the STK district is sacrosanct, and highlight the necessity to analyze compositional and contextual factors influencing willingness to accept and adopt solar water disinfection systems. Our findings will inform policies and programs aimed at implementing solar water disinfection systems in the study area to improve the health of communities that must rely on poor quality drinking water sources.