bluuebunny/crossref_metadata_2025 huggingface dataset:
Next Generation Sequencing Based Comprehensive Molecular Analysis in Iranian Patients with Restless Legs Syndrome (RLS)
Authors: ALIBABAEI ZAHRA , Hesampour Ardeshir , Hamdi Seyed.Mohammad.Mehdi , Najafi Mohsen , mohammadpour Hadiseh , Alizadeh Rasoul , Alibabaei Batoul
Published: June 2021

Restless legs syndrome (RLS) is a common sensory-motor neurodegenerative disorder. It usually occurs at night and every 10 to 60 seconds with unpleasant sensations in the legs, burning and itching. Need to move and feel Uncomfortable legs improve with movement.In 20% of patients, symptoms can also include the arm and other parts of the body, which often occur in a more severe form of the disease. But generally, according to research, this syndrome is a multifactorial syndrome. These factors are divided into genetic and environmental or medical factors.In this study, this disorder was investigated genetically and molecularly and the genes affecting the disease in Iranian population were identified by NGS method.Materials and Methods: In this study, NGS and SEQUENCING methods were used to investigate the relationship between genes that influence disease and patients.Conclusion: In this study, we found that ATXN7, MEIS1 and TANC1 genes are effective in restless legs syndrome. In this study, In this study, the effect of TANC gene on the development of restless legs syndrome in Iranian families was confirmed.Discussion: The results of molecular analysis of restless legs syndrome using NGS method led to the identification of new gene loci on ATXN7 and TANC1 genes in Iranian population. Also, gene loci on MEIS1 gene, previous studies on its involvement in restless legs syndrome Confirmed.
The Role of Carbonic Anhydrase Ⅲ and Autophagy in Type 2 Diabetes with Cardio-Cerebrovascular Disease
Authors: Zhang Xiaoming , Tao Ying-Hong , Zhou Xiu-Ling , Shang Xi-Liang , Gong Xiao-Bo , Liu Ying-Chao , Huang Yan-Yan , Chen Gang , Yu Zhong-Yu , Wang Jian-Tao , Du Zun-Guo , Wu Guo-Feng , Zhang Yu , Guo Jing-Chun , Zhou HouGuang
Published: June 2021

BackgroundType 2 diabetes mellitus (T2DM) is one of the most common chronic diseases among the elderly people. The T2DM increases the risk of cardio-cerebrovascular disease (CCD), and the main pathological change of the CCD is atherosclerosis (AS). Meanwhile, the carbonic anhydrases (CAs) are involved in the formation and progression of plaques in AS. However, the exact physiological mechanism of carbonic anhydrase Ⅲ (CAⅢ) has not been clear yet, and there are also no correlation study between CAⅢ protein and T2DM with CCD. MethodsThe 8-week old diabetic mice (db/db−/− mice) and wild-type mice (wt mice) were feed by a normal diet till 32 weeks, and detected the carotid artery vascular opening angle using the method of biomechanics; The changes of cerebral cortex and myocardium were watched by the ultrastructure, and the autophagy were observed by electron microscope; The tissue structure, inflammation and cell injury were observed by Hematoxylin and eosin (HE) staining; The apoptosis of cells were observed by TUNEL staining; The protein levels of CAⅢ, IL-17, p53 were detected by immunohistochemical and Western Blot, and the Beclin-1, LC3, NF-κB were detected by Western Blot. All statistical analysis is performed using PRISM software.ResultsCompared with wt mice, db/db−/− mice’ carotid artery open angle increased significantly. Electron microscope results indicated that autophagy in db/db−/− mice cerebral cortex and heart tissue decreased and intracellular organelle ultrastructure were damaged. HE staining indicated that, db/db−/− mice’ cerebral cortex and heart tissue stained lighter, inflammatory cells infiltration, cell edema were obvious, myocardial fibers were disorder, and myocardial cells showed different degrees of degeneration. Compared with wt mice, TUNEL staining showed that there was obviously increase in db/db−/− mice cortex and heart tissue cell apoptosis. The results of immunohistochemistry and Western Blot indicated that CAⅢ, Beclin-1 and LC3Ⅱ/Ⅰ expression levels conspicuously decreased in cortex and heart tissue of db/db−/− mice, and the expression level of IL-17, NF-κB and p53 obviously increased.ConclusionThere were obvious AS in the carotid artery of db/db−/− mice. And the autophagy participated in the occurrence and development of diabetic CCD. CAⅢ protein might somehow be involved in the regulation of autophagy probably through affecting cell apoptosis and inflammation, but the underlying mechanism remains to be further studied.
Cellulose Nanospheres Coated Polylactic Acid Nonwoven Membranes for Recyclable Use in Oil/Water Separation
Authors: Wen Jiexiu , Yu Qihao , Cui Chaofan , Su Juanjuan , Han Jian
Published: June 2021

Oily wastewater has always been an environmental issue that we are concerned about and committed to managing. Although the superwetting membranes have been extensively studied and widely used in oil/water separation, the abundant discarded membranes are still headaches due to their non-degradable nature. In this work, all-around green, as well as superhydrophilic polylactic acid (PLA) nonwoven membrane, is designed by coating cellulose nanospheres (CNCs) with the aid of polydopamine (PDA). Abundant hydroxyl groups and hierarchical rough structure synergistically contribute to the superhydrophilicity and excellent oil/water separation performance of the resultant CNCs/PDA/PLA nonwoven membrane. Interestingly, the oil/water separation efficiency can be maintained above 98% after 100 times of repeated use. With outstanding durability, and easy controllability, the CNCs/PDA/PLA nonwoven membrane may provide effective solutions to simultaneously manage the oily wastewater and secondary pollution of the used membrane itself.
Negative Cost Girth Problem Using Map-Reduce Framework
Authors: Shamsi Mahboubeh , Kenari Abdolreza Rasouli , Aghamohammadi Roghayeh
Published: June 2021

On a graph with a negative cost cycle, the shortest path is undefined, but the number of edges of the shortest negative cost cycle could be computed. It is called Negative Cost Girth (NCG). The NCG problem is applied in many optimization issues such as scheduling and model verification. The existing polynomial algorithms suffer from high computation and memory consumption. In this paper, a powerful Map-Reduce framework implemented to find the NCG of a graph. The proposed algorithm runs in O(log k) parallel time over O(n3) on each Hadoop nodes, where n; k are the size of the graph and the value of NCG, respectively. The Hadoop implementation of the algorithm shows that the total execution time is reduced by 50% compared with polynomial algorithms, especially in large networks concerning increasing the numbers of Hadoop nodes. The result proves the efficiency of the approach for solving the NCG problem to process big data in a parallel and distributed way.
Oxaliplatin Compromised CDK1 Activity Sensitizes BRCA-Proficient Cancers to PARP Inhibition in Oxaliplatin Resistance Gastric Cancer
Authors: Li Huafu , Wang Chunming , Lan Linxiang , Wu Wenhui , Evans Ian , Ruiz E Josue , Yan Leping , Zhang Haiyong , Zhou Zhijun , Hu Zhenran , Chen Wei , Behrens Axel , He Yulong , Zhang Changhua
Published: June 2021

Background: Oxaliplatin resistance is one of the most important problems in the treatment of cancer. The successful culture of tumor organoid in gastric cancer can help us to study oxaliplatin resistance and its mechanism. Thus, it is convenient for us to successfully solve oxaliplatin resistance and improve the prognosis of patients.Methods: Two oxaliplatin resistant patients and two oxaliplatin sensitive patients were enrolled through our Gastric Cancer Center of Sun Yat-sen University. Core genes of oxaliplatin resistant and non-resistant patients were analyzed by sequencing. The overexpression and knockdown of core genes were carried out by organoid in vivo, combined with oxaliplatin-resistant cell lines AGS, MKN74 and SNU719 for cell viability, WB and immunofluorescence, etc., to verify the role of core genes in oxaliplatin resistance. Again, in vivo experiments were verified by subcutaneous tumor formation in vitro.Results: Through sequencing, we found that PARP1 is an important core gene leading to oxaliplatin resistance. In vivo organoids, oxaliplatin resistant cell lines and subcutaneous tumor formation in vivo. We found that PARP1 was an important cause of oxaliplatin resistance. Oxaliplatin can inhibit CDK1 activity and make cancer with normal BRCA1 function sensitive to PARP inhibition. Through the combination of oxaliplatin and PARP1 inhibitor olaparib, we can effectively kill tumor cells. Through the patients' follow-up data, we found that the expression level of PARP1 was significantly correlated with oxaliplatin resistance.Conclusion: Our results indicate that PARP1 is an important core gene leading to oxaliplatin resistance. Combined oxaliplatin and PARP1 inhibitor olaparib can effectively kill tumor cells. Oxaliplatin can inhibit CDK1 activity and make cancers with normal BRCA1 function more sensitive to PARP inhibitors.
Phytotoxic Effect Of Aqueous Extracts of Different Plant Parts Of Sphaeranthus Indicus On Germination And Seedling Growth Of Eleasine Coracana
Authors: Kumbhar B. A. , Dabgar P. J.
Published: June 2021

BackgroundThe phytotoxic influences of water extract of different plant parts of Sphaeranthus indicus (Burandu) were tested by growing test crop of Eleasine coracana (Nagli) for seedling growth. Significant differences in the growth of the test crop were observed. ResultThe hypocotyl growth of test crop depressed significantly at high concentration (6%) aqueous extract of different plant parts of S. indicus was found after 10 days. Redicle length of Nagli crop was stimulated in 2% shoot aqueous extract of Sphaeranthus indicus whereas other concentrations of all aqueous extracts of Burandu weed show phytotoxic effects as compared to control after ten days. Shoot and Root dry weights of food crop were reduced by Burandu residue. ConclusionTest crop Nagli was more sensitive to root aqueous extract while less sensitive to shoot aqueous extract of Sphaeranthus indicus.
Long-Term Adverse Effects of Cigarette Smoking on the Incidence Risk of Metabolic Syndrome: Longitudinal Findings of the Korean Genome and Epidemiology Study Over 12 Years
Authors: Kim Ae Hee , Seo In-Ho , Lee Hye Sun , Lee Yong-Jae
Published: June 2021

BackgroundPrevious studies have demonstrated positive associations between smoking and metabolic syndrome (MetS) in several cross-sectional studies. However, the association was not consistent in long-term studies, which makes longitudinal effects remain controversial. Thus, we investigated the association between cigarette smoking and incidence risk of MetS in a large-sample, community-based, longitudinal prospective study in both intensity and cumulative dose of cigarette smoking over 12 years of follow-up. MethodsAmong 10,038 participants, a total of 5568 men ages 40-69 years old without MetS at baseline were selected from the Korean Genome and Epidemiology Study (KoGES). The hazard ratios (HRs) and 95% confidence interval (CIs) for incident MetS were calculated using a multivariate Cox proportional-hazards regression model after adjusting for potential confounding variables and setting never smokers as the reference group for intensity (expressed as number of cigarettes per day) and duration of smoking (expressed as number of pack-years). Results Compared to the referent never smokers, the HRs (95% CIs) for incident MetS increased as the intensity of smoking in current smokers also increased: 1.50 (1.07-2.01) for 0-9 cigarettes/day, 1.66 (1.34-2.06) for 10-19 cigarettes/day, and 1.75 (1.34-2.29) for ≥20 cigarettes/day, after adjusting for age, alcohol drinking, physical activity, household income, educational level, mean arterial pressure, triglyceride, HDL-cholesterol level, and homeostasis model assessment insulin resistance. These positive relationships were similar when the cumulative dose of smoking was used in current smokers. Compared to the referent never smokers, the HRs (95% CIs) for incident MetS increased as the cumulative dose also increased: 1.63 (1.32-2.02) for <20 PYs and 1.67 (1.30-2.14) for 20 ≥PYs after adjusting for the same co-variables. ConclusionsBoth cigarette smoking intensity and cumulative dose were positively associated with MetS among community-dwelling Korean men in the large-scale, longitudinal, prospective, 12-year follow-up study.
Litter inputs and phosphatase activity control the temporal variability of organic phosphorus in a tropical forest soil in the Central Amazon
Authors: Schaap Karst J. , Fuchslueger Lucia , Hoosbeek Marcel R. , Hofhansl Florian , Martins Nathielly Pires , Valverde-Barrantes Oscar J. , Hartley Iain P. , Lugli Laynara F. , Quesada Carlos Alberto
Published: June 2021

Purpose. The tropical phosphorus-cycle and its impacts on phosphorus (P) availability are a major uncertainty in projections of forest productivity. In highly weathered soils with low P concentrations, plant and microbial communities depend on biological and physical processes to acquire P. We explored the seasonality and relative importance of drivers controlling the fluctuation of common P pools via processes such as litter production and decomposition, and soil phosphatase activity. Methods. We analyzed variation of standard tropical soil phosphorus pools over one year. In addition, we measured litterfall, its decomposition rates and soil extracellular phosphatase enzyme activity and tested their relation to the fluctuations in P-fractions. Results. Our results show clear patterns of seasonal variability of soil P fractions during the year. We found that modeled P released during litter decomposition is positively related to change in organic P fractions, while net change in organic P fractions was negatively related to phosphatase activities in the top 5 cm. Conclusion. We conclude that input of organic P forms by litter decomposition and organically produced phosphatases are the two main factors controlling seasonal soil P fluctuation, and therefore the P economy in P impoverished soils. Organic soil P follows a clear seasonal pattern, indicating tight cycling of the nutrient, while reinforcing the importance of studying soil P as an integrated dynamic system in a tropical forest context.
Ultrafast Synthesis of MoS2 Quantum dot Particles using Low Energy at Room Temperature
Authors: Wu Jun Yi
Published: June 2021

In this study, a new method of synthesizing large amount highly fluorescent MoS2 quantum dots is presented. This low environmental impact ,ultrafast ,saving energy method can synthesize a large number of MoS2 quantum dots(QDs). Using the NaCl crystal particles sharp edge that are insoluble in the supersaturated NaCl solution combined with the shear force generated by the press shaker to cut the MoS2 bulk material to form MoS2 QDs.Large amount highly fluorescent MoS2 quantum dots in a matter of 3 min using the NaCl crystal as a sustainable cutting agent.The prepared MoS2 QDs were characterized by TEM ,FESEM ,EDX ,DLS ,XPS, UV-vis Spectroscopy ,Florescence Spectroscopy analyses. Furthermore, this ultrafast, low energy consumption,green process may offer a potential major advance for production of other QDs materials from bulk material.
Is a Stop Solution Necessary for Metal Nanoparticles Aggregation-based Colorimetric Assays?
Authors: Jouyban Abolghasem , samadi azam
Published: June 2021

We report herein for the first time that a stop solution should be used to terminate the metal nanoparticles aggregation reactions especially salt-induced ones to increase the repeatability of the assays. It was found that hydrophobic surfactants have the ability to slow down the aggregation process of MNPs and can be applied as stop solution for aggregation based colorimetric assays. This trick provides accurate and well-repeatable signals since the aggregation of the nanoparticles is a dynamic process and capturing signal in a predetermined time is difficult.
Aquatic Macrophytes in Southern Amazonia, Brazil: Richness, Endemism, and Comparative Floristics
Authors: Neyra Milton Omar Cordova , Keffer Josiane Fernandes , Giacoppini Dienefe Rafaela , Pott Vali Joana , Pott Arnildo , Munhoz Cássia Beatriz Rodrigues
Published: June 2021

Southern Amazonia potentially harbors a wide diversity of aquatic macrophyte species because of its diverse wetland habitats and location in the Amazon-Cerrado transition zone, the two largest biogeographic domains in South America. We inventoried the taxonomic diversity of aquatic macrophytes in the region, including endemism, habitats, species life forms, and floristic comparisons with other Brazilian regions. We found a high number of species of aquatic macrophytes for the southern Amazon region, comparable to extensive inventories in larger areas in southern, northeastern, and northern regions of Brazil. We recorded 688 species of aquatic macrophytes in 299 genera and 95 families, of which 66 species are new records, 89 endemic, and 5 exclusive to Brazilian Amazonia. We present new records of aquatic macrophyte species, together with their degree of endemism and occurrence restricted to the region. The macrophyte species list of southern Amazonia showed 60% similarity to inventories in the Xingu basin and in northern Brazil. This high diversity of aquatic macrophytes in the Southern Amazonia, with endemic species and others with restricted ranges, emphasizes the importance of conserving wetlands and vegetation types where they grow in the region.
Dietary Carbohydrate Intake and New-onset Diabetes: A Nationwide Cohort Study in China
Authors: Zhou Chun , Zhang Zhuxian , Liu Mengyi , Zhang Yuanyuan , Li Huan , He Panpan , Li Qinqin , Liu Chengzhang , Qin Xianhui
Published: June 2021

Background: The association of carbohydrate intake with diabetes risk remains uncertain. We aimed to evaluate the prospective associations of the amount and types of carbohydrate intake with new-onset diabetes.Methods: A total of 16,260 non-diabetic participants from the China Health and Nutrition Survey (CHNS) were included. Dietary intake was collected by three consecutive 24-h dietary recalls combined with a household food inventory. Participants with self-reported diabetes, or fasting plasma glucose ≥7.0 mmol/L or glycated hemoglobin ≥6.5% during the follow-up were defined having new-onset diabetes. Results: During a median follow-up of 9 years (158,930 person-years), 1,100 participants developed diabetes. Overall, there was a U-shaped association between percent of energy from carbohydrate intake and new-onset diabetes, with minimal risk at 49-56% of energy from total carbohydrate intake (quartile 2) (P for nonlinearity <0.001). Moreover, there was an L-shaped association between high-quality carbohydrate intake and new-onset diabetes (P for nonlinearity <0.001), and a J-shaped association of low-quality carbohydrate intake with new-onset diabetes (P for nonlinearity <0.001). Furthermore, there was an inverse association between the plant-based low-carbohydrate scores for low-quality carbohydrate and new-onset diabetes. However, a reversed J-shaped association was found between the animal-based low-carbohydrate scores for low-quality carbohydrate and new-onset hypertension (P for nonlinearity <0.001). Conclusions: There was a U-shape association between percent of total carbohydrate intake and new-onset diabetes, with the lowest risk at 49-56% carbohydrate intake. Our findings provide some evidence for the intake of high-quality carbohydrate, and the substitution of plant-based products for low-quality carbohydrate for primary prevention of diabetes.
Upregulation of SGOL2 Predicts Poor Prognosis and Facilitates Hepatocellular Carcinoma Progression via Dysregulating the Cell Cycle by Directly Activating MAD2
Authors: Hu Qingqing , Hu Xiaochu , Zhao Yalei , Zhang Lingjian , Yang Ya , li lanjuan
Published: June 2021

Background: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide. Shugoshin-like protein 2 (SGOL2) is a centromeric protein that ensures the correct and orderly process of mitosis by protecting and maintaining centripetal adhesions during meiosis and mitosis. However, the role of SGOL2 in cancer is not well understood. Methods: The mRNA and protein levels of SGOL2 and survival analysis were conducted in The Cancer Genome Atlas (TCGA) and further validated in 2 independent cohorts. Differential genes correlated with SGOL2 and mitotic arrest deficient 2 like 1 (MAD2) were obtained using LinkedOmics. Subsequently, loss-of-function and rescue assays were carried out in vitro and in vivo to assess the functions of SGOL2 in hepatic tumorigenisis. Findings: We found that SGOL2 was significantly overexpressed in HCC and predicted unfavorable overall survival in HCC patients. Next, we identified 47 differentially expressed genes positively correlated with both SGOL2 and MAD2 to be mainly involved in the cell cycle. In addition, SGOL2 downregulation suppressed the migration, invasion, proliferation, stemness and EMT of HCC cells and inhibited tumorigenesis in vivo. Furthermore, SGOL2 promoted tumor proliferation by activating MAD2-induced cell cycle dysregulation, which could be reversed by the MAD2 inhibitor M2I-1. We also proved that SGOL2 activated MAD2 by directly binding with MAD2. Conclusions: The results of this study showed that SGOL2 acts as an oncogene in HCC cells by directly activating MAD2 and then dysregulating the cell cycle, thereby providing a potential target for HCC patients in the future.
Decreasing Formaldehyde Emission from Particleboard Panels Fabricated by Adding Newly Phenolic Compounds as a Scavenger to Urea Formaldehyde Resin
Authors: kord behzad , Movahedi Farnaz , Adlnasab Laleh , Ayrilmis Nadir
Published: June 2021

This study focused on the influence of some novel scavengers on the physical, mechanical, and formaldehyde emission of particleboard produced with urea-formaldehyde resin. Three different scavengers, alizarin red sulfonate, alizarin yellow-GG, and chromotropic acid, were incorporated to the UF resin at 1, 3, 5, and 7% (by weight) loading levels based on the oven-dried weight of the resin. Other manufacturing parameters were kept constant in the producrion of particleboards. The results indicated that the formaldehyde emission of the particleboards significantly reduced with increasing amount of the scavengers. The lowest formaldehyde emission was found in the specimens containing alizarin red sulfonate (0.38 mg/l), followed by chromotropic acid (0.43 mg/l), and alizarin yellow-GG (0.49 mg/l), respectively, at 7 wt% loading level of the scavengers. Although the physical and mechanical properties of the particleboards decreased with the increasing content of the scavengers, they met the requirements of particleboards for interior fitments (including furniture) for use in dry conditions (P2 grade) of EN 312 standard. According to the results of technological properties and formaldehyde emission of the particleboards, it can be said that alizarin red sulfonate is the best scavenger among the investigated scavengers.
Neuropsychiatric Functioning in CDLS: A Detailed Phenotype and Genotype Correlation
Authors: Ajmone Paola Francesca , Allegri Beatrice , Cereda Anna , Michelini Giovanni , Dall'Ara Francesca , Mariani Milena , Rigamonti Claudia , Selicorni Angelo , Vizziello Paola , Costantino Maria Antonella
Published: June 2021

Background: Cornelia de Lange Syndrome (CdLS) is a rare genetic disorder and its phenotype clinical expression is widely variable. Despite the growing interest on genotype-phenotype correlations and on behaviour phenotype of genetic syndromes, specific studies in CdLS cohorts evaluating the correlations between genotype and neurodevelopmental characteristics, Behaviour and communicative aspects are limited, most of these studies are descriptive and there is a lack of specific assessment protocols.Methods: Neurodevelopmental and Behavioural phenotype of all the patients (N = 38) was assessed using a specific neuropsychiatric protocol, concerning Intellectual Quotient (IQ), General Quotient of Development (GQ), communicative skills, behavioural aspects and adaptive behaviour based on direct and indirect evaluation. Subsequently, we searched for possible genotype–phenotype correlations comparing individuals with NIPBL variants (CdLS NIPBL mutated group) and patients with negative molecular results (CdLS clinical diagnosis group). Results: The first part of the study showed a higher percentage of subjects with normal Intellectual Quotient (IQ) and Borderline Intellectual Functioning if compared with previous data; adaptive skills were lower than expected for age in all participants and the weakest areas were Socialization, Motor Skills, and Communication. Expressive language was more compromised than receptive language, nevertheless receptive abilities were also impaired. 39.5% of the sample presented with Autism Spectrum Disorder (ASD). By stratifying CdLS phenotypes by genetic, NIPBL mutated individuals demonstrated a worse trend in cognitive functioning, in communication expressive skills, in motor skills and in ASD symptoms in comparison with the Clinical Diagnosis Group. Individuals with non-Truncating mutation (mostly missense) displayed better abilities in Communication and in relational aspects with no ASD while Truncating individuals presented with worse abilities in Daily Living Skills, Socialization, Motor Skills, and Communicative abilities. Conclusions: These findings should increase our awareness of the strengths and weaknesses points in CdLS individuals to guide appropriate targeted management; interventions addressing communicative impairments represent a clinical priority in CdLS patients.
Categorization of Patients With Systemic Lupus Erythematosus Using Disease Activity, Patient-Reported Outcomes and Transcriptomic Signatures
Authors: Arcani Robin , Jouve Elisabeth , Chiche Laurent , Jourde-Chiche Noemie
Published: June 2021

Objective

Patients with systemic lupus erythematosus (SLE) display symptoms that are not always related to disease activity and may distort clinical trial results. Recently, a clinical categorization based on the presence of type 1 (inflammatory manifestations) and/or type 2 (widespread pain, fatigue, depression) symptoms has been proposed in SLE. Our aim was to develop a type 2 score derived from the Short-Form health survey (SF-36) to categorize SLE patients and to compare immunological and transcriptomic profiles between groups.
Methods

Seventeen items from the SF-36 were selected to build a type 2 score for 50 SLE patients (100 visits; LUPUCE cohort) and the SLEDAI was used to define type 1 symptoms. Patients were categorized in four groups: minimal (no symptoms), type 1, type 2 and mixed (both type 1 and type 2 symptoms). Clinical, immunological and transcriptomic profiles were compared between the groups.
Results

Type 2 scores ranged from 0 to 31, with a cut-off value of 14 (75th percentile). The sample categorization was: minimal in 39%, type 1 in 37%, type 2 in 9% and mixed in 15%. Type 2 patients were older than minimal patients and had a longer disease duration than type 1 and mixed patients. Immunological data and modular interferon signatures did not differ between the groups.
Conclusion

Patients with SLE can be categorized into four clinical groups using the SLEDAI score and our SF-36-derived type 2 score. This categorization is non-redundant with immunological or transcriptomic profiles and could prove useful to stratify patients in clinical trials.
Reference Gene Selection for RT-qPCR Assays in Different Tissues of Huperzia Serrata Based on Full-Length Transcriptome Sequencing
Authors: Fu Yanping , Niu Fei , Jia Hui , Wang Yanli , Feng Yana , Guo Bin , He Wei , Wei Yahui
Published: June 2021

Background

Huperzia serrata (H. serrata) produces various types of effectively lycopodium alkaloids, especially Huperzine A (HupA), which is a promising drug for the treatment of Alzheimer’s disease. Numerous studies focused on the chemistry, bioactivities, toxicology, and clinical trials of HupA, however, the public genomic and transcriptomic resources are very limited for H. serrata research, especially for the selection of optimum reference genes.
Results

Based on the full-length transcriptome datasets and previous studies, thirteen candidate reference genes were selected in different tissue of H. serrata. Then, two optimal reference genes GAPDHB and HisH2A were confirmed by various analysis softwares. In order to further verify the accuracy of the two reference genes, they were used to analyze the expression patterns of four HupA-biosynthetic related genes (lysine decarboxylas, RS-norcoclaurine 6-O-methyltransferase, cytochrome P45072A1 and copper amine oxidase). The data suggested that the expression trends of HupA-biosynthetic related genes were consistent with them in transcriptome sequencing in different tissue of H. serrata.
Conclusions

This study screened the best reference genes GAPDHB and HisH2A in different tissues of H. serrata, which provides suitable normalization for analyzing the expression of HupA-biosynthetic gene in transcriptional level in H. serrata.
Lung Ultrasonography Decreases Radiation Exposure in Newborns with Respiratory Distress
Authors: Tandircioglu Umit Ayse , Yigit Sule , Oguz Berna , Kayki Gozdem , Celik Hasan Tolga , Yurdakok Murat
Published: June 2021

Chest X-ray(CXR) is commonly used as a first line imaging method to diagnose the reason of respiratory distress in NICUs.Lung ultrasound is a new diagnostic tool for lung imaging. We aimed to determine the decrease in the number of CXRs on the first day of life in newborns with respiratory distress,with the use of lung ultrasonography. From January 2019 to June 2020,104 newborn infants hospitalized in the NICU with respiratory distress on the first day of life enrolled in this study(ClinicalTrials.govIdentifier NCT04722016).We used ultrasound as the first line technique for lung imaging.CXR was taken to determine endotracheal tube and umbilical catheter position or if considered necessary by the physician in charge of the infant.We calculated decreased number of CXR for every patient and evaluated the estimated decrease in radiation exposure. 104 neonates with median 36 weeks(25–40)gestational age and birth weight 2410gr(600–4100) enrolled in the study.Seventy(67,3%)of these babies were male.In the study group,24(23,1 %)patients were diagnosed with respiratory distress syndrome(RDS),49(47,1 %) patients with transient tachypnea of newborn(TTN),27(26 %) with pneumonia,4(3,8 %)with congenital heart diseases.Lung ultrasonography were performed 210 times for all infants,but CXRs were performed a total of only 107 times.CXR wasnot taken in 27 of the patients with a diagnosis of TTN,in 2 of the patients with a diagnosis of congenital pneumonia,and in one of the patients with congenital heart disease.The rate of patients who have never had a chest x-ray was 28,8%.Conclusions:We observed that usage of lung ultrasonography decreased the number of chest X-ray and radiation exposure in newborns with respiratory distress.
Effect of Amylolytic and Cellulolytic Enzymes on Whole Plant Corn Silage: Characteristics of Silage and Animal Digestion
Authors: GANDRA JEFFERSON RODRIGUES , Nunes Alanne T. , Oliveira Euclides R. , Silva Mávio S. J. , Pedrini Cibeli A. , Machado Fabio S. , Silva Geleice K. R. , Gandra Erika R. S. , Mendes Paulo V. C. , Pause Alzira G. S.
Published: June 2021

Enzyme additives have been added to forage at ensiling to improve nutritive value. The aim of this study was to evaluate effects of adding exogenous enzymes to silage on fermentative losses and profile, aerobic stability, chemical composition, in vitro degradation, microbial quality, and nutrients intake and digestibility. Treatments were control (CON); addition of exo-1,4-α-glycosidase glucoamylase (GLU; Kerazyme 3035, Kera Nutrição Animal, Bento Gonçalves, Brazil); addition of β-glucan 4-glucanhydrolase (CEL); and GLU + CEL. Data from the silo experiment were analyzed using PROC MIXED of SAS, with fixed effects of glucoamylase and cellulase, and interaction effect between them. In vivo experiment analyses also included fixed effect of Latin Square and period, and random effect of animal within Latin Square. CEL increased (P ≤ 0.038) gas losses and effluents production, CEL and GLU decreased (P = 0.039) DM recovery compared to control but not differ from GLU+CEL. CEL silage had higher (P ≤ 0.021) starch and crude protein and in vitro digestibility of DM and NDF (P ≤ 0.032), while GLU had higher (P = 0.001) ADF. CEL showed lower (P = 0.012) ethanol content and higher (P = 0.02) anaerobic bacteria counts, while GLU showed higher (P = 0.012) lactate concentration and lower (P ≤ 0.002) counts of bacteria and fungi. Lambs fed with CEL presented higher (P ≤ 0.012) digestibility coefficients for DM, OM, CP and NDF. Decrease on DM recovery indicates no improvements on the nutritive value of silage. On the other hand, cellulolytic enzyme positively affected animal digestion.
Primary Pericardial Angiosarcoma: A Case Report and Literature Review
Authors: sun hong , zhao min
Published: June 2021

Primary angiosarcoma is extremely rare malignant tumor that has no typical symptoms and progress rapidly with poor prognosis. It is mesenchymal in origin and observed most frequently in the right atrium, cases in the pericardium is much more rare. Only few can detected in the early-stage allowing complete radical resection with a mean survival of 3 months to 1 year. There is few pericardial angiosarcoma reported among these years. The present study reports a case of a 44-year-old woman with primary pericardial angiosarcoma, who underwent a wide range of imaging methods, including transthoracic echocardiography, contrast-enhanced computed tomography (CT) and positron emission tomography-magnetic resonance imaging (PET-MRI). The patient recovered well after operation in two years and died due to the recrudescence and pulmonary metastases in April, 2020. We report the case for its rarity and revealing the early detection of primary pericardial angiosarcoma with imaging examinations is critical for prognosis. Finally a literature review is done.
MiR-323a-3p Inhibits Tumor Growth and Gefitinib Resistance Acquisition by Targeting EGFR/ErbB3 in Colorectal Cancer
Authors: Zhang Yuanzhou , Liang Shunshun , Xiao Bowen , Hu Jingying , Pang Yechun , Liu Yuling , Yang Juan , Ao Junpin , Wei Lin , Luo Xiaoying
Published: June 2021

Background: EGFR-TKIs are prone to develop acquired drug resistance in colorectal cancer and are only applicable in Kras wild type colorectal cancer patients. This study aimed to determine the reasons for the poor treatment efficacy of TKIs in Kras mutant CRC and to improve the treatment effect. Method: The RTK Phosphorylation Membrane array was used to detect and screen changes in phosphorylated protein levels in KRAS mutant-resistant CRC cells. qRT-PCR, western blot and TCGA database were applied for reporting the expression of ERGR and ErbB3 in CRC. Luciferase reporter and western blot examined the network of miR-323a-3p. RTCA, colony formation, CCK-8, caspase-3/7 activity and Flow cytometry probed the impacts of miR-323a-3p on CRC cell growth.Results: We illustrated that ErbB3 and EGFR were activated in gefitinib-resistant Kras mutant colorectal cancer cell lines. ErbB3 is highly expressed in Kras mutant patient tissues, and patients with ErbB3high/EGFRhigh had a poorer prognosis. Mechanically, We found and verified that the tumor suppressor miR-323a-3p simultaneously directly targeted EGFR/ErbB3 and inhibited tumor cell growth by activating the apoptosis pathway. Further functionality studies identified miR-323a-3p synergized with gefitinib to inhibit tumor growth, and this synergy prevented the development of acquired resistance to gefitinib in CRC cell lines.Conclusion: Accordingly, these data indicate that Kras mutant CRC TKI resistance occurs due to the activation of EGFR and ErbB3. Thus, miR-323a-3p has the potential to treat Kras-mutated colorectal cancer by targeting ErbB3/EGFR.
Influence Of Geometric Attribute On Friction Coefficient In Sheet Metal Stamping
Authors: Zhao Dewang , Zhao Kunmin , Song Huaihao , Ren Daxin , Chang Ying
Published: June 2021

This paper presents a test device to explore the influence of geometric attribute of the contact surface on friction coefficient along with sliding speed and contact pressure. Friction tests were conducted on a third-generation high strength steel QP980. The friction coefficients for different surface curvatures, contact pressures and sliding speeds were calculated and the influences of these factors were analyzed. The formula for calculating the friction coefficients between curved contact surfaces was derived. The relationship between bending induced surface roughness increase and friction coefficient was established. An enhanced friction coefficient model with pressure, velocity and curvature dependence was proposed. The enhanced friction model was applied to simulate the stamping of an automotive part and a better correlation was achieved.
The Correlation Between IgM and IgG Antibodies With Blood Profile In Patients Infected With COVID 19
Authors: Alibolandi Zahra , Ostadian Amirreza , Sayyah Saeed , Kashani Hamed Haddad , Ehteram Hassan , Banafshe Hamid Reza , Hajijafari Mohammad , Sepehrnejad Mahdi , Kashani Narjes Riahi , Azadchehr Mohammd-Javad , Nikzad Hossein , Hosseini Elahe Seyed
Published: June 2021

Objectives: This study aimed to determine the levels of IgM and IgG antibody response to the severe acute respiratory syndrome coronavirus (SARS-CoV)-2 in coronavirus disease 2019 (COVID-19) patients with different disease severity.Methods: IgM and IgG antibody levels were evaluated via enzyme-linked immunosorbent assay (ELISA). In total, 100 patients with confirmed SARS-CoV-2 infection were enrolled in this study and viral RNA was detected by using Real-time PCR technique. Clinical and laboratory data were collected and analyzed after hospital admission for COVID-19 and two months post-admission. Results: The level of anti-SARS-CoV-2 antibody IgG was significantly higher in the severe patients than those in moderate and mild groups, 2 months after admission. Also, level of IgG was positively associated with increased WBC, NUT and LYM counts in sever than mild or moderate groups after admission to hospital.Conclusion: Our findings suggested that patients with severe illness might experience longer virus exposure times and have a stronger antibody response against viral infection. Thus, they have longer time immunity compared with other groups.
Lifetime Attributable Risk Estimates of Secondary Cancer after External Radiotherapy among Colorectal Cancer Survivors in Saudi Arabia
Authors: Abolaban Fouad , Djouider Fathi
Published: June 2021

Radiation-induced second cancer is one of the crucial late side effects of radiotherapy treatment of first cancer. Although the second cancer induction mechanism is not well understood yet, many factors are related to its occurrences, such as age at exposure, dose to the organ and surrounding tissues, treatment modalities, and family history of cancer. This study aims to provide long-term estimates of second cancer incidence amongst colon cancer survivors in Saudi Arabia. The lifetime attributable risk (LAR) after radiation treatment of the colon cancer was determined, between the age at exposure and up to 95 years, in a single-institution cohort of male and female cancer survivors whose age at treatment was in the range 43 to 85 years. Risk estimates varied significantly with age at exposure, gender, and organ dose.
Rice (Oryza Sativa L.) Whole Grain Genome-Wide Association Study for Neutral Sugar Content
Authors: Panahabadi Rahele , McKee Lauren S. , Ahmadikhah Asadollah , Ingvarsson Pär K. , Farrokhi Naser
Published: June 2021

Background Cell wall matrix polysaccharides are structurally complex and diverse, but our knowledge about their synthesis is limited. The building blocks (monosaccharides) of these polysaccharides have critical role in defining the number and the ultrastructure (size) of rice grains, and therefore would have great influence on seed vigor, yield and quality. Genome-wide association study (GWAS) is a method of choice for fine mapping of QTLs involved in defining plant cell wall structure and modifications. The monosaccharides that contribute to major cell wall matrix polysaccharides in whole grains of 197 rice accessions were quantified using acid hydrolysis and high-performance anion-exchange chromatography with pulsed amperometric detection. A GWAS of calculated monosaccharide content in rice whole grain (RWG) was carried out using 33,812 single-nucleotide polymorphisms (SNPs) to identify corresponding markers in 200 kbp flanking regions.Results In total, 49 significant SNPs contained in 19 genomic regions (QTLs) on eight chromosomes of rice were determined to be associated with monosaccharides content. The candidate genes in QTL regions included the following: arabinose content was associated with α-N-arabinofuranosidase, pectinesterase inhibitor, a glycoside hydrolase (GH) from family 16 (GH16 enzyme); xylose content was associated with ethylene-responsive element-binding protein and a pyruvate kinase; mannose content was associated with a MYB family transcription factor and S-domain receptor-like protein kinase; galactose content was associated with a glycocyltransferase (GT) from family 8 (GT8 enzyme), GRAS family transcription factor, glutathione S-transferase, GH16 and GH17 enzymes; fucose content was associated with a GH16 enzyme, lysine-rich arabinogalactan protein 19 and a receptor protein kinase; and finally rhamnose content was associated with OsFBX41, pectinesterase, COBRA-like protein, and OsSAUR13 (Auxin-responsive SAUR).Conclusion The results of this study should improve our understanding of the genetic basis of the factors that might be involved in the biosynthesis and turnover of major matrix polysaccharides present in RWG. Several QTLs were identified on different chromosomes, all are reported for first. Further, our data provide insight that will be useful in the design of future breeding programs, allowing breeders to use available genetic resources more effectively in meeting global food demand and supply.
Genome-Wide Identification and Expression Analysis of Flavonoid 3'-Hydroxylase Gene Family in Carthamus Tinctorius L.
Authors: Quốc Hoàng Việt Nguyễn , Jie Kong , Ahmad Naveed , Zhuoda Yang , Nan Wang , Xintong Ma , Yue Zhang Xin , Xiuming Liu , Na Yao , Libo Jin
Published: June 2021

ObjectiveThrough experiments and bioinformatic analysis clearly demonstrate considerable information about the genetic evolution of the flavonoid 3'-hydroxylase (F3'H) gene in Safflower and in plants.ResultsHere, we performed genome wide survey of safflower genome and identified a total of 22 CtF3'H enzyme encoding genes. Phylogenetic analyses revealed the classifications of these CtF3'Hs into nine subgroups demonstrating their evolutionary relationship. The distribution of the conserved protein motifs, and cis-regulatory units of CtF3'Hs indicated essential structure-to-function components leading to the final function of protein or its interactions. Furthermore, the results of digital expression analysis and the qRT-PCR pattern of 22 putative CtF3’H genes during different flowering stages suggested their requisite roles in safflower petal pigmentation. In addition, the fusions construct of plant expression vector pCAMBIA1302-GFP-CtF3’H5 in onion epidermal cells verified the subcellular localization of CtF3’H5 to the plasma membrane. Subsequently, the prokaryotic expression and western blot hybridization of CtF3’H5 resulted in a stable 50.3kD target protein. These results partly demonstrate the influence of F3'Hs on plants.ConclusionsIn this study, the results of digital expression and qRT-PCR analysis of 22 putative CtF3'H genes in different flowering stages indicate their essential role in safflower petal pigmentation. clearly demonstrates significant information on the genetic evolution of important enzyme-coding genes and will provide a pathway for future functional studies of F3'Hs in safflower as well as in plants.
Uncovering of the Association Between m5C Regulator-Mediated Methylation Modification Patterns and Tumor Microenvironment Infiltration Characteristics in Hepatocellular Carcinoma
Authors: Gu Xinyu , Zhou Haibo , Chu Qingfei , Zheng Qiuxian , Wang Jing , Zhu Haihong
Published: June 2021

Background: 5-Methylcytosine (m5C) plays essential roles in hepatocellular carcinoma (HCC), but the association between m5C regulation and immune cell infiltration in HCC has not yet been clarified.Methods: In this study, we analysed 371 patients with HCC from The Cancer Genome Atlas (TCGA) database, and the expression of 13 m5C regulators was investigated. Additionally, gene set variation analysis (GSVA), unsupervised clustering analysis, single-sample gene set enrichment analysis (ssGSEA), correlation analysis, and immunohistochemical (IHC) staining were performed.Results: Among the 371 patients, 41 had mutations in m5C regulators, the frequency of which was 11.26%. Then, we identified three m5C modification patterns that had obvious tumour microenvironment (TME) cell infiltration characteristics. Cluster-1 had an immune rejection phenotype; Cluster-2 had an immunoinflammatory phenotype; and Cluster-3 had an immune desert phenotype. In addition, we found that DNMT1 was highly expressed in tumour tissues compared with normal tissues in a tissue microarray (TMA) and that it was positively correlated with many TME-infiltrating immune cells. High expression of the m5C regulator DNMT1 was related to a poor prognosis in patients with HCC. Furthermore, we developed three Immu-clusters that were consistent with the immune characteristics of the m5C methylation modification patterns. We also discovered differences in the levels of immune cells and expression of chemokines and cytokines among the three Immu-clusters.Conclusions: Our work revealed the association between m5C modification and immune regulators in the TME. These findings also suggest that DNMT1 has great potential as a prognostic biomarker and therapeutic target for HCC.