bluuebunny/crossref_metadata_2025 huggingface dataset:
Gene Panel Sequencing Identifies a Novel RYR1 p.Ser2300Pro Variant as Candidate for Malignant Hyperthermia with Multi-Minicore Myopathy
Authors: Moon Young Jae , Park Joonhong , Kim Jung Ryul , Lee Seung Yeob , Lee Jaehyeon , Cho Yong Gon , Kim Dal Sik
Published: September 2022

Malignant hyperthermia (MH), a rare autosomal dominant pharmacogenetic disorder of skeletal muscle calcium regulation, is triggered by sevoflurane in susceptible individuals. We report a Korean having MH with multi-minicore myopathy functionally supported by RYR1-mediated intracellular Ca2+ release testing in B lymphocytes. A 14-year-old boy was admitted for the evaluation of progressive torticollis accompanied by cervicothoracic scoliosis. During the preoperative drape of the patient for the release of the sternocleidomastoid muscle under general anesthesia, his wrist and ankle were observed to have severe flexion contracture. The body temperature was 37.1 °C. To treat MH, the patient was administered a bolus of dantrolene intravenously (1.5 mg/kg) and sodium bicarbonate. After a few minutes, muscle rigidity, tachycardia, and EtCO2 all resolved. Next-generation panel sequencing for hereditary myopathy identified a novel RYR1 heterozygous missense variant (NM_000540.2: c.6898T > C; p.Ser2300Pro), which mapped to the MH2 domain of the protein, a hot spot for MH mutations. Ex vivo RYR1-mediated intracellular Ca2+ release testing in B lymphocytes showed hypersensitive Ca2+ responses to isoflurane and caffeine, resulting in an abnormal Ca2+ release only in the proband, not in his family members. Our findings expand the clinical and pathological spectra of information associated with MH with multi-minicore myopathy.
Meta-Analysis of SNPs Determining Litter Traits in Pigs
Authors: Sell-Kubiak Ewa , Dobrzanski Jan , Derks Martijn F. L. , Lopes Marcos S. , Szwaczkowski Tomasz
Published: September 2022

Nearly 2000 SNPs associated with pig litter size traits have been reported based on genome-wide association studies (GWASs). The aims of this study were to gather and integrate previously reported associations between SNPs and five litter traits: total number born (TNB), number born alive (NBA), number of stillborn (SB), litter birth weight (LWT), and corpus luteum number (CLN), in order to evaluate their common genetic background and to perform a meta-analysis (MA) of GWASs for total number born (TNB) recorded for animals from five pig populations. In this study, the genes with the largest number of associations with evaluated litter traits were GABRG3, RBP7, PRKD1, and STXBP6. Only 21 genes out of 233 associated with the evaluated litter traits were reported in more than one population or for more than one trait. Based on this evaluation, the most interesting candidate gene is PRKD1, which has an association with SB and TNB traits. Based on GO term analysis, PRKD1 was shown to be involved in angiogenesis as well. As a result of the MA, two new genomic regions, which have not been previously reported, were found to be associated with the TNB trait. One SNP was located on Sus scrofa chromosome (SSC) 14 in the intron of the FAM13C gene. The second SNP was located on SSC9 within the intron of the AGMO gene. Functional analysis revealed a strong candidate causal gene underlying the QTL on SSC9. The third best hit and the most promising candidate gene for litter size was found within the SOSTDC1 gene, associated with lower male fertility in rats. We showed that litter traits studied across pig populations have only a few genomic regions in common based on candidate gene comparison. PRKD1 could be an interesting candidate gene with a wider association with fertility. The MA identified new genomic regions on SSC9 and SSC14 associated with TNB. Further functional analysis indicated the most promising gene was SOSTDC1, which was confirmed to affect male fertility in other mammals. This is an important finding, as litter traits are by default linked with females rather than males.
Integrative System Biology Analysis of Transcriptomic Responses to Drought Stress in Soybean (Glycine max L.)
Authors: Shahriari Amir Ghaffar , Soltani Zahra , Tahmasebi Aminallah , Poczai Péter
Published: September 2022

Drought is a major abiotic stressor that causes yield losses and limits the growing area for most crops. Soybeans are an important legume crop that is sensitive to water-deficit conditions and suffers heavy yield losses from drought stress. To improve drought-tolerant soybean cultivars through breeding, it is necessary to understand the mechanisms of drought tolerance in soybeans. In this study, we applied several transcriptome datasets obtained from soybean plants under drought stress in comparison to those grown under normal conditions to identify novel drought-responsive genes and their underlying molecular mechanisms. We found 2168 significant up/downregulated differentially expressed genes (DEGs) and 8 core modules using gene co-expression analysis to predict their biological roles in drought tolerance. Gene Ontology and KEGG analyses revealed key biological processes and metabolic pathways involved in drought tolerance, such as photosynthesis, glyceraldehyde-3-phosphate dehydrogenase and cytokinin dehydrogenase activity, and regulation of systemic acquired resistance. Genome-wide analysis of plants’ cis-acting regulatory elements (CREs) and transcription factors (TFs) was performed for all of the identified DEG promoters in soybeans. Furthermore, the PPI network analysis revealed significant hub genes and the main transcription factors regulating the expression of drought-responsive genes in each module. Among the four modules associated with responses to drought stress, the results indicated that GLYMA_04G209700, GLYMA_02G204700, GLYMA_06G030500, GLYMA_01G215400, and GLYMA_09G225400 have high degrees of interconnection and, thus, could be considered as potential candidates for improving drought tolerance in soybeans. Taken together, these findings could lead to a better understanding of the mechanisms underlying drought responses in soybeans, which may useful for engineering drought tolerance in plants.
Combination of Serum and Plasma Biomarkers Could Improve Prediction Performance for Alzheimer’s Disease
Authors: Zhang Fan , Petersen Melissa , Johnson Leigh , Hall James , O’Bryant Sid E.
Published: September 2022

Alzheimer’s disease (AD) can be predicted either by serum or plasma biomarkers, and a combination may increase predictive power, but due to the high complexity of machine learning, it may also incur overfitting problems. In this paper, we investigated whether combining serum and plasma biomarkers with feature selection could improve prediction performance for AD. 150 D patients and 150 normal controls (NCs) were enrolled for a serum test, and 100 patients and 100 NCs were enrolled for the plasma test. Among these, 79 ADs and 65 NCs had serum and plasma samples in common. A 10 times repeated 5-fold cross-validation model and a feature selection method were used to overcome the overfitting problem when serum and plasma biomarkers were combined. First, we tested to see if simply adding serum and plasma biomarkers improved prediction performance but also caused overfitting. Then we employed a feature selection algorithm we developed to overcome the overfitting problem. Lastly, we tested the prediction performance in a 10 times repeated 5-fold cross validation model for training and testing sets. We found that the combined biomarkers improved AD prediction but also caused overfitting. A further feature selection based on the combination of serum and plasma biomarkers solved the problem and produced an even higher prediction performance than either serum or plasma biomarkers on their own. The combined feature-selected serum–plasma biomarkers may have critical implications for understanding the pathophysiology of AD and for developing preventative treatments.
Identification of Cdk8 and Cdkn2d as New Prame-Target Genes in 2C-like Embryonic Stem Cells
Authors: Lucci Valeria , De Marino Elena , Tagliaferri Daniela , Amente Stefano , Pollice Alessandra , Calabrò Viola , Vivo Maria , Falco Geppino , Angrisano Tiziana
Published: September 2022

Embryonic stem cells (ESCs) present a characteristic pluripotency heterogeneity correspondent to specific metastates. We recently demonstrated that retinoic acid (RA) induces an increase in a specific 2C-like metastate marked by target genes specific to the two-cell embryo stage in preimplantation. Prame (Preferentially expressed antigen in melanoma) is one of the principal actors of the pluripotency stage with a specific role in RA responsiveness. Additionally, PRAME is overexpressed in a variety of cancers, but its molecular functions are poorly understood. To further investigate Prame’s downstream targets, we used a chromatin immunoprecipitation sequencing (ChIP-seq) assay in RA-enriched 2C-like metastates and identified two specific target genes, Cdk8 and Cdkn2d, bound by Prame. These two targets, involved in cancer dedifferentiation and pluripotency, have been further validated in RA-resistant ESCs. Here, we observed for the first time that Prame controls the Cdk8 and Cdkn2d genes in ESCs after RA treatment, shedding light on the regulatory network behind the establishment of naïve pluripotency.
De Novo Assembly Transcriptome Analysis Reveals the Preliminary Molecular Mechanism of Primordium Formation in Pleurotus tuoliensis
Authors: Wang Chunxia , Zhou Jinkan , Cao Zijian , Hu Bao , Wang Jing , Guo Jinying , Zheng Suyue
Published: September 2022

Primordium formation is extremely important for yield of Pleurotus tuoliensis. However, the molecular mechanism underlying primordium formation is largely unknown. This study investigated the transcriptional properties during primordium formation of P. tuoliensis by comparing transcriptome. Clean reads were assembled into 57,075 transcripts and 6874 unigenes. A total of 1397 differentially expressed genes were identified (26 DEGs altered in all stages). GO and KEGG enrichment analysis showed that these DEGs were involved in “oxidoreductase activity”, “glycolysis/gluconeogenesis”, “MAPK signaling pathways”, and “ribosomes”. Our results support further understanding of the transcriptional changes and molecular processes underlying primordium formation and differentiation of P. tuoliensis.
Profiling Players Involved in Overkill: An Analysis of 71 Cases in Central Italy
Authors: Tavone Alessandro Mauro , Ceccobelli Giulia , Piizzi Giorgia , Vella Raimondo , Giuga Gabriele , Cammarano Andrea , Petroni Giulia , Marella Gian Luca
Published: September 2022

“Overkill” is characterized by the finding of excessive wounds on the victim’s body. Despite the large use of this term, it does not have a definition in the literature yet. Our study aimed to analyze the information related to the dynamics of overkill cases, collecting objective variables, and producing a profile of the players involved in this type of homicides. Data on 71 overkill cases from reports of the autopsies performed in the Section of Legal Medicine of the University of Rome Tor Vergata from 1 January 2000 to 31 December 2020 were collected. The victims and the perpetrators of overkill shared similar characteristics: they were usually male aged between 20 and 50 years, more likely in the 20–35 years range; victims however also showed another age peak in the range 50–55 years. The type of damage can be linked to the sole action of a cold weapon or the simultaneous use of multiple harmful tools with no significant differences. The most common motive proved to be a dispute for futile reasons; however, in the case of a single perpetrator of the crime, the economic and passionate motives are as frequent as the previous one. Implications of the findings and avenues for future research are discussed.
Preventive Medication Use among Adults Aged 40 and over in the United States: National Health and Nutrition Examination Survey, 2015–2018
Authors: Meraya Abdulkarim M.
Published: September 2022

Objectives: 1. To estimate the prevalence of preventive medication use among adults in the United States (US); 2. To identify the socioeconomic, demographic and clinical factors associated with preventive prescription medication use; 3. To identify the diagnoses associated with preventive prescription medication use. Methods: Data from two cycles of the National Health and Nutrition Examination Survey, 2015–2016 and 2017–2018, are analyzed. Results: Among US adults aged 40 years or older (N = 7634), 31% use a preventive medication. Specifically, 27% of them use aspirin and 9% use other preventive prescription medications. Among those who use other preventive prescription medications, 27% report using one of the cardiovascular agents and 24% reported using anticoagulants and/or antiplatelet agents. High percentages of preventive prescription medication users report using medications to prevent heart attacks/myocardial infarctions (25%) or blood clots (23%). Uninsured adults are less likely to use preventive medications (OR: 0.656; p-value = 0.009) as compared with their counterparts with private insurance. On the other hand, older adults and those with diabetes, heart disease, arthritis and hypertension are more likely to use preventive medications. Furthermore, past smokers and adults who never smoked are more likely to use preventive medications as compared with those who currently smoke. Conclusion: Policies are needed to increase access to preventive care for uninsured adults. Further research needs to review the benefits and harms of the chronic use of preventive medication among older adults in the US.
Exploring Women’s Experiences of Maternity Service Delivery in Regional Tasmania: A Descriptive Qualitative Study
Authors: Hargreaves Sally , Young Sarah , Prior Sarah J. , Ayton Jennifer
Published: September 2022

The objective of this study is to explore and understand the experiences of women who receive antenatal, birthing, and postnatal care from an integrated maternity services model in a regional area in Tasmania, Australia. This descriptive qualitative study included semi-structured, one-on-one interviews with 14 mothers aged >18 years, who were living in a regional area of Tasmania and had accessed maternity health services. Thematic analysis revealed three key themes: (i) talking about me, (ii) is this normal? and (iii) care practices. Overall, women cited mostly negative experiences from a poorly implemented fragmented service. These experiences included feelings of isolation, frustration over receiving conflicting advice, feeling ignored, and minimal to no continuity of care. In contrast, women also experienced the euphoric feelings of birth, immense support, guidance, and encouragement. Regional women’s experiences of maternity care may be improved if health services work towards place-based continuity of care models. These models should be informed by the local women’s experiences and needs in order to achieve better communication, reduce feelings of isolation, and promote positive breastfeeding experiences.
What Does It Mean to Truly Empathize with a Patient? An Analysis of Japanese Cases Employing the Narrative Approach Perspective
Authors: Akabayashi Akira , Nakazawa Eisuke
Published: September 2022

What does it mean to truly empathize with a patient? The authors (a psychiatrist and a philosopher) explore this topic from the unique perspectives gained from decades of experience. We discuss how some approaches that may be criticized are necessary if we are to empathize with a patient. We also touch on the current situation surrounding personnel involved in the restoration of the Fukushima Daiichi Nuclear Power Plant (the so-called Fukushima 50) after the nuclear meltdown caused by the Great East Japan Earthquake in 2011. We conclude with what we have learned to truly emphasize with patients from these cases: (1) small deviations seem to be useful sometimes; (2) healthcare professionals do not have to be too hard on themselves to empathize with patients, and a natural, narrative approach-based attitude is sometimes more than enough; and (3) physical stance, in addition to psychological stance, might also be a factor. Moreover, we look at the idea of the “village society” and argue that it is necessary for medical professionals to be fully aware of the negative connotations of village societies.
The Effectiveness of Physical Activity Interventions on Depression in Korea: A Systematic Review and Meta-Analysis
Authors: Lee Ye Hoon , Kim Hyungsook , Cho Heetae
Published: September 2022

Since the onset of the COVID-19 pandemic, Korea has ranked first in the OECD, with a prevalence of 36.8% of depression. Thus, this study aimed to estimate the effect size of physical activity as an alternative tool for depression symptoms using meta-analysis. A meta-analysis on depressive symptoms was performed on 18 studies published in Korean domestic journals. The moderating variables hypothesized in this study included age groups of participants; depressive symptoms; and frequency, intensity, time, type, and duration of the intervention. The overall effect size of physical activity on depression was moderate (0.56 [95% CI: 0.39 to 0.91]). Specifically, physical activity was slightly more effective in reducing depression in participants with an 18–64 age group compared to older people over 65 years old, while it was most effective for participants without depressive symptoms compared to participants with mild and severe symptoms. Further, the subgroup analysis revealed that performing two times a week for 30 to 60 min with progressive intensity for 1–8 weeks may be the most effective for Koreans. The results of this study can provide guidelines for the most effective physical activity program for Koreans.
The Impact of Mild COVID-19 on Executive Functioning and Mental Health Outcomes in Young Adults
Authors: Manukyan Piruza , Deviaterikova Alena , Velichkovsky Boris B. , Kasatkin Vladimir
Published: September 2022

COVID-19 is associated with a range of long-lasting symptoms related to cognitive and psycho-emotional spheres. Even mild cases of the disease can lead to persistent cognitive deficits and deterioration of the psycho-emotional state. The purpose of our study was to examine the presence and frequency of psycho-emotional disorders and cognitive deficits in students who recovered from mild form of COVID-19. A total of 40 COVID-19 survivors and 25 healthy controls participated in our study. We assessed three core cognitive functions (inhibition, working memory, task-switching), reaction time and motor speed. We also assessed depression, anxiety and fatigue with self-report questionnaires. The COVID-19 group manifested increased depression and decreased inhibition in comparison with the controls. Our results show that even in young adults who have recovered from mild COVID-19, there are persisting cognitive and psycho-emotional deficits.
Studying Saraha: Technical and Multi-Analytical Investigation of the Painting Materials and Techniques in an 18th Century Tibetan Thangka
Authors: Dyer Joanne , Derham Alice , O’Flynn Daniel , Tamburini Diego , Heady Teresa , Ramos Imma
Published: September 2022

Scientific analyses of the traditional materials and methods in thangka production are uncommon, as thangkas are sacred objects, the sampling of which is discouraged, in order to preserve their integrity. This study builds on this important ethical challenge and presents a three-stage methodology that systematically delves deeper into each layer of the composition, successfully enabling the investigation of different phases of production. In the first stage, visual examination of the painting, including observations under magnification, was used to assess its condition. In the next step, the infrared reflected (IRR) and short-wave infrared (SWIR) images revealed the underdrawing and instances of modifications as well as colour notations. Additionally, ultraviolet-induced visible luminescence (UVL), infrared-reflected false colour (IRRFC) and X-ray images provided important preliminary information on the colourants present, the nature of the underdrawing, and the painting technique. In the final stages, fibre optic reflectance (FORS), Fourier-transform infrared (FTIR) and Raman spectroscopies confirmed the identity of many of the pigments (cinnabar/vermillion, minium, iron oxide, malachite, azurite, indigo, Indian lac), the ground and the binder used for the blue and green paint layers. In addition, key details of practices and materials were revealed, that may indicate provenance or other information of scholarly importance. These will constitute a helpful comparison to existing and future studies of other thangkas.
Strawberry Tree Fruits and Leaves (Arbutus unedo L.) as Raw Material for Sustainable Functional Food Processing: A Review
Authors: Bebek Markovinović Anica , Brčić Karačonji Irena , Jurica Karlo , Lasić Dario , Skendrović Babojelić Martina , Duralija Boris , Šic Žlabur Jana , Putnik Predrag , Bursać Kovačević Danijela
Published: September 2022

The strawberry tree (Arbutus unedo L.) is a Mediterranean plant known for the traditional use of its fruits and leaves due to their health benefits. Thus, it has been used for years in folk medicine to relieve various health conditions such as urological and kidney problems, dermatological, cardiovascular and gastrointestinal diseases. The fruits are traditionally used for making jams, jellies, and strong alcoholic beverages, while the leaves are mostly used for preparing tea. Since the leaves were more researched, previous results indicated that they have important biological effects, so further research should focus on the fruits. Due to its chemical composition, rich polyphenolic profile and the biological potential derived from it, the plant has great prospects for the production of functional foods and nutraceuticals. However, the plant’s potential is underutilized in terms of processing. Therefore, this review summarizes the properties and the potential of the fruits and leaves of A. unedo and their possible benefits for processing with respect to agricultural, nutritive, biological and economic values.
Ground-Penetrating Radar Detection of Hydrologic Connectivity in a Covered Karstic Setting
Authors: Honings Joseph P. , Wicks Carol M. , Brantley Steven T.
Published: September 2022

Increasing demand for water for agricultural use within the Dougherty Plain of the southeastern United States has depleted surface water bodies. In karstic landscapes, such as the Dougherty Plain in southwest Georgia where the linkages between surface and ground waters are close, there is a need to understand the physical characteristics of the subsurface that allow these close linkages. Having a better understanding of the subsurface characteristics will aid numerical modeling efforts that underpin policy decisions and economic analyses. Two common features on this karstic landscape are draws and geographically isolated wetlands. Using LiDAR, aerial imagery, and ground-penetrating radar, this study investigates the subsurface characteristics of a draw and a series of geographically isolated wetlands. GPR reflections indicative of karst features are laterally continuous and connect the landscape to the nearby Ichawaynochaway Creek. The identification of the size and scale of the laterally continuous karstic features will guide the implementation of groundwater models used to determine irrigation and forest restoration programs while minimizing the impacts of water use on surface streams and the ecosystems.
Monkeypox Diagnosis by Cutaneous and Mucosal Findings
Authors: de Sousa Diogo , Patrocínio João , Frade Joana , Brazão Claúdia , Mancha Dora , Correia Catarina , Borges-Costa João , Filipe Paulo
Published: September 2022

A monkeypox outbreak has been reported in several countries since early May 2022. Human monkeypox (MPX) diagnosis is based on a clinical suspicion supported by typical skin and mucosal lesions, confirmed with molecular testing. We present the results of all MPX confirmed patients presenting to our department until July 15 of 2022, describing the characteristics of the lesions at diagnosis. In total, 47 patients were included, all men and 44.7% (n = 21) were HIV-positive. Skin lesions were noted in all patients. The most commonly affected area was the genital region (63.8%), followed by the anorectal region (46.8%). Extra anogenital mucosal (oral or conjunctival mucosa) involvement was reported in three patients. Typical skin findings included erythematous papules, whitish, umbilicated papules, some with a necrotic center and an elevated whitish border. Most patients had lesions in multiple phases presenting simultaneously. Correct identification of MPX skin and mucosal lesions is crucial to avoid late diagnosis and prevent further spreading, ensuring less worldwide morbidity.
Certain Dietary Nutrients Reduce the Risk of Eye Affliction/Retinopathy in Individuals with Diabetes: National Health and Nutrition Examination Survey, 2003–2018
Authors: Zhang Guoheng , Sun Xiaojia , Yuan Tianhao , Guo Changmei , Zhou Ziyi , Wang Ling , Dou Guorui
Published: September 2022

As the global trend of diabetes intensifies, the burden of vision-threatening retinopathy, particularly diabetic retinopathy (DR), is increasing. There is an urgent need to seek strategies for early prevention and control of DR. This study attempted to comprehensively evaluate the relationship between dietary nutrient intake and the risk of DR to provide assistance for doctors in guiding the diet of diabetic patients. Data from eligible participants with diabetes from the US National Health and Nutrition Examination Survey (NHANES) from 2003–2018 were analyzed. Univariate logistic regression was used to assess the association between 58 dietary nutrient intakes and self-reported eye disease risk. Multivariate logistic regression model was used to further evaluate the relationship between the two groups after adjusting relevant confounding factors. A total of 4595 diabetic patients were included. People with self-reported eye affliction/retinopathy had lower dietary fiber, butanoic, octanoic, vitamin A, alpha-carotene, folate, magnesium, copper and caffeine intake compared to those without self-reported eye affliction/retinopathy. The pooled ORs (95% CIs) were 0.78 (0.62–0.98), 0.79 (0.63–0.99), 0.72 (0.58–0.91), 0.74 (0.59–0.93), 0.70 (0.55–0.88), 075 (0.60–0.95), 0.79 (0.64–0.99), 0.67 (0.54–0.84) and 0.80 (0.64–0.99). Dietary cholesterol and hexadecenoic intake were higher, with the pooled ORs (95% CIs) of 1.26 (1.01–1.58) and 1.27 (1.02–1.59), respectively. Our research found that among dietary nutrients, dietary fiber, butanoic, octanoic, vitamin A, alpha-carotene, folate, magnesium, copper and caffeine intake reduced the occurrence of DR. Cholesterol and hexadecenoic intake promoted the occurrence of DR. This suggests that certain dietary nutrients should be paid more attention in the prevention of DR.
Health Promotion Programs Can Mitigate Public Spending on Hospitalizations for Stroke: An Econometric Analysis of the Health Gym Program in the State of Pernambuco, Brazil
Authors: da Guarda Flávio Renato Barros
Published: September 2022

Health promotion programs can reduce morbidity and mortality from chronic diseases, as well as public spending on health. The current study aims to evaluate the effects of the Health Gym Program on expenditures on hospitalizations for stroke in the state of Pernambuco, Brazil. This public policy impact assessment used an econometric model that combines the difference-in-difference estimator with propensity score matching. Data referring to the health, demographic, and socioeconomic characteristics of the 185 municipalities in Pernambuco were collected for the period from 2007 to 2019. Validation tests were carried out of the empirical strategy, the estimation of models with fixed effects for multiple periods and validation post-tests, and robustness of the results. In total, US$ 52,141,798.71 was spent on hospitalizations for stroke, corresponding to 4.42% of the expenses on hospitalizations for all causes over the period studied. Municipalities that implemented the Health Gym Program spent 17.85% less on hospitalizations for stroke than municipalities that did not. The findings of this study indicate that the Health Gym Program was effective in reducing expenses with hospitalizations for stroke and that its implementation has the potential to reduce expenses related to rehabilitation, sick leave, and early retirement.
Effect of Core Balance Training on Muscle Tone and Balance Ability in Adult Men and Women
Authors: Jo Sun-Ha , Choi Hyuk-Jae , Cho Hyeon-Seok , Yoon Jin-Hwan , Lee Won-Young
Published: September 2022

(1) Background: The amount of physical activity most adults perform is less than the recommended amount, and the resulting decrease in physical strength makes them vulnerable to various diseases. A decrease in muscle size and strength due to damage caused by disease or aging negatively affects functional strength. Muscle evaluation in adults can yield results that are predictive indicators of aging and unexpected disability. In addition, balance ability is essential to prevent falls and injuries in daily life and maintain functional activities. It is important to develop and strengthen balance in the lower extremities and core muscles to maintain and enhance overall body balance. This study aimed to analyze the effects of core balance training on muscle tone and balance ability in adults. (2) Methods: The participants of this study were 32 adult male and female university students (male: mean age = 21.3 ± 1.9 years, weight = 74.2 ± 12.6 kg, BMI = 23.4 + 2.5, n = 14; female: mean age = 21.0 ± 1.4 years, weight = 64.6 + 1.2 kg, BMI = 22.4 ± 2.4, n =18). Thirty-two adults (training group: 16, control group: 16; male: 16, female: 16) participated in the Myoton PRO (gastrocnemius lateral/medial, tibialis anterior), Pedalo balance system, and Y-balance test. (3) Results: The following results were obtained for muscle elasticity, stiffness, and dynamic/static balance ability after 10 weeks of core balance training. 1. There was no significant difference in muscle elasticity (gastrocnemius lateral/medial, tibialis anterior) (p < 0.05). 2. Muscle stiffness (gastrocnemius lateral/medial, tibialis anterior) significantly increased (p < 0.05). 3. Dynamic/static balance ability significantly increased (p < 0.05). (4) Conclusions: In future, data for the age and sex of various participants, should be accumulated by recruiting participants to study muscle characteristics, such as muscle elasticity and stiffness. Estimating the appropriate injury range and optimal exercise capacity is possible through follow-up studies. The findings can then be used as a basis for predicting injuries or determining and confirming the best time to resume exercise.
Association between Climate Factors and Dengue Fever in Asuncion, Paraguay: A Generalized Additive Model
Authors: Gómez Gómez Raquel Elizabeth , Kim Jeehyun , Hong Kwan , Jang Jin Young , Kisiju Trishna , Kim Soojin , Chun Byung Chul
Published: September 2022

Dengue fever has been endemic in Paraguay since 2009 and is a major cause of public-health-management-related burdens. However, Paraguay still lacks information on the association between climate factors and dengue fever. We aimed to investigate the association between climatic factors and dengue fever in Asuncion. Cumulative dengue cases from January 2014 to December 2020 were extracted weekly, and new cases and incidence rates of dengue fever were calculated. Climate factor data were aggregated weekly, associations between dengue cases and climate factors were analyzed, and variables were selected to construct our model. A generalized additive model was used, and the best model was selected based on Akaike information criteria. Piecewise regression analyses were performed for non-linear climate factors. Wind and relative humidity were negatively associated with dengue cases, and minimum temperature was positively associated with dengue cases when the temperature was less than 21.3 °C and negatively associated with dengue when greater than 21.3 °C. Additional studies on dengue fever in Asuncion and other cities are needed to better understand dengue fever.
Can the Establishment of National Key Ecological Function Areas Enhance Vegetation Carbon Sink? A Quasi-Natural Experiment Evidence from China
Authors: Zhang Tongyue , Hou Mengyang , Chu Liqi , Wang Lili
Published: September 2022

The National Key Ecological Functional Areas (NKEFAs) of China rely on the main functional area planning, with the core goal of enhancing the supply of ecological products. Carbon sink is an important ecological product, and it is necessary to understand whether the establishment of NKEFAs has enhanced vegetation carbon sink (CS). Considering the establishment of NKEFAs as a quasi-natural experiment, based on the panel data of prefecture-level cities in China from 2001 to 2019, a time-varying difference-in-differences (DID) model is used to systematically examine the impact of NKEFAs on CS. The study found that the establishment of NKEFAs has significantly enhanced the CS, and compared to the non-NKEFAs, NKEFAs has increased CS in the covered areas by an average treatment effect (ATE) of 2.1625. The establishment of NKEFAs can enhance CS through the optimization of territory spatial structure, the upgrading of industrial structure and the inter-industrial mobility of labor. The enhancement roles of NKEFAs on CS are heterogeneous across different functional area types, geospatial locations, and quantile levels, with higher enhancement of CS at windbreak–sand fixation type, northwestern region and high quantiles, respectively. In addition, NKEFAs not only have a significant positive ecological spillover effect, but also balanced with local economic growth, they achieve the goals of “lucid waters and lush mountains are invaluable assets”.
Research on Carbon Emission Efficiency Space Relations and Network Structure of the Yellow River Basin City Cluster
Authors: Song Haihong , Gu Liyuan , Li Yifan , Zhang Xin , Song Yuan
Published: September 2022

The Yellow River Basin serves as China’s primary ecological barrier and economic belt. The achievement of the Yellow River Basin’s “double carbon” objective is crucial to China’s green and low-carbon development. This study examines the spatial link and network structure of city cluster carbon emission efficiency in the Yellow River Basin, as well as the complexity of the network structure. It focuses not only on the density and centrality of the carbon emission efficiency network from the standpoint of city clusters, but also on the excellent cities and concentration of the city cluster ‘s internal carbon emission efficiency network. The results show that: (1) The carbon emission efficiency of the Yellow River Basin has been dramatically improved, and the gap between city clusters is narrowing. However, gradient differentiation characteristics between city clusters show the Matthew effect. (2) The distribution of carbon emission efficiency in the Yellow River Basin is unbalanced, roughly showing a decreasing trend from east to west. Lower-level efficiency cities have played a significant role in the evolution of carbon emissions efficiency space. (3) The strength of the carbon emission efficiency network structure in the Yellow River Basin gradually transitions from weakly correlated dominant to weakly and averagely correlated dominant. Among them, the Shandong Peninsula city cluster has the most significant number of connected nodes in the carbon emission efficiency network. In contrast, the emission efficiency network density of the seven city clusters shows different changing trends. Finally, this study suggests recommendations to improve carbon emission efficiency by adopting differentiated governance measures from the perspective of local adaptation and using positive spatial spillover effects.
Assessment of the Quality of Life of Women after Osteoporotic Vertebral Fracture with Consideration of Socio-Demographic Characteristics and Selected Factors Concerning the State of Health
Authors: Dziedzic Małgorzata , Janiszewska Mariola , Goździewska Małgorzata , Kowalska Wioleta , Roliński Jacek
Published: September 2022

Introduction: Fractures of the vertebral bodies are a frequent complication of osteoporosis, hospitalization, decline in physical fitness and, in consequence, deterioration in the quality of life. Objective: The aim of the study was assessment of the quality of life according to the QUALEFFO-41 questionnaire in patients who had undergone fractures of the vertebral bodies, and presentation of the relationships between the quality of life, socio-demographic characteristics, and selected factors concerning the state of health. Materials and Method: The study included 243 women with osteoporotic vertebral fractures, and was conducted in the Outpatient Departments for the Treatment of Osteoporosis in the city of Lublin (eastern Poland). For the purposes of the study, the Quality of Life Questionnaire (QUALEFFO-41) and the author’s questionnaire were employed, and Spearman’s rank correlation coefficient, t-Student test, and Tukey test were used, along with analysis of variance (ANOVA). The level of statistical significance was set at α = 0.05. Results: The quality of life of woman with vertebral compression fractures remains on a mediocre level. Significant relationships were observed between the respondents’ quality of life and certain socio-demographic characteristics, duration of the disease, and complaints related with osteoporosis. Conclusions: It is important to implement appropriate therapy and provide comprehensive, holistic care to women after fractures.
Acceptability of the Vaccine against COVID-19 in Spanish Health Sciences Students: A Cross-Sectional Study
Authors: Rodríguez-Blanco Noelia , Vicente-Alcalde Nancy , Cubero-Plazas Laura , Sánchez-Más Jesús , Montagud Emilia , Moragues Raul , Gabaldón-Bravo Eva , Hurtado-Sanchez Jose Antonio , Tuells José
Published: September 2022

Healthcare professionals must play an exemplary role in the field of vaccinology. It is convenient that they are trained during their time at university. The objective of this study was to determine the acceptability of the vaccines against COVID-19 in health sciences students in Spanish universities. A cross-sectional study was performed regarding the acceptance of the vaccines against COVID-19 in students in the Health Sciences Degrees in Spanish universities was performed on a sample of students of nursing, medicine, and pharmacy during the spring of 2021, via an online questionnaire with 36 questions designed ad hoc, self-administered, anonymized, and standardized. There were 1222 students participating, of Spanish nationality (97.4%), women (80.5%) and with an average age of 22.0 ± 4.8 years old. Of those, 12.3% had had the disease, 44.0% had to quarantine, 70.8% had undergone diagnostic tests, out of which 14.1% were positive. In total, 97.5% of those surveyed indicated their desire of being vaccinated, if possible, with Comirnaty® (74.9%). At the time of the study, 49.6% were already vaccinated. The reasons for vaccination differed according to the degree and the doubts about vaccine safety was the largest reason for reluctance. Some 37.7% suspected that there are unknown adverse effects and 85.6% of those vaccinated experienced some mild effects after injection. Vaccine acceptance and confidence in the recommendations given by health authorities is high in health sciences students.
Factors Associated with Improved Knowledge of Metabolic Syndrome in Female Market Traders
Authors: Achempim-Ansong Gloria , Tshabalala Amme M. , Gradidge Philippe J.
Published: September 2022

Metabolic syndrome (MetS) is considered to be a clustering of cardiometabolic diseases and is emerging as a public health concern. There is little evidence of this disease in market traders, and so the aim of this study was to determine the prevalence and knowledge of MetS. In this cross-sectional study, anthropometry, blood pressure and bloods were collected using standardized methods to detect the prevalence of MetS using the harmonized method in a cohort of female Ghanaian market traders (n = 338). A questionnaire documented the knowledge of MetS. Linear regression was used to investigate the factors associated with knowledge and was reported as adjusted β values. Forty-two percent (n = 142) had MetS. The overall knowledge of MetS was low, driven by education (β = 0.22, p = 0.0001), low levels of high-density lipoprotein-cholesterol (β = −0.15, p = 0.018) and affiliation with the Ewe cultural group (β = −0.19, p = 0.0004). As females working in a sedentary occupation, market traders are vulnerable to MetS. Our findings indicate the urgent need for culturally sensitive education to promote healthy behaviours.
Unclear Insomnia Concept in Randomized Controlled Trials and Systematic Reviews: A Meta-Epidemiological Study
Authors: Banno Masahiro , Tsujimoto Yasushi , Kohmura Kunihiro , Dohi Eisuke , Taito Shunsuke , Someko Hidehiro , Kataoka Yuki
Published: September 2022

There are two possible ways to conceptualize the term “insomnia”: insomnia disorder and insomnia symptoms, which are often poorly reported. The purpose of this study was to examine the proportion of randomized controlled trials (RCTs) and systematic reviews (SRs) that mention insomnia in their abstracts and cannot distinguish between insomnia disorder and insomnia symptoms from the abstract. We included RCT and SR articles that included the word “insomnia” in the methods or results sections of their structured abstracts, published after 2010. We searched PubMed using English language restrictions on 10 March 2022. From 1580 PubMed articles, we obtained 100 random samples each for eligible RCTs and SRs. The unclear insomnia concept accounted for 88% of the RCT abstracts and 94% of the SR abstracts. Among the RCT and SR abstracts with unclearness, the concept of insomnia was unclear in 27% of RCTs and 57% of SRs after investigating the full text. The concept of insomnia has been unclear in many RCTs and SRs abstracts. The authors of RCTs and SRs are recommended to state “insomnia disorder” or “insomnia symptoms” in the methods and results sections of their abstracts.
Assessment of a Multifunctional River Using Fuzzy Comprehensive Evaluation Model in Xiaoqing River, Eastern China
Authors: Fu Yongfei , Liu Yuyu , Xu Shiguo , Xu Zhenghe
Published: September 2022

Rivers are beneficial to humans due to their multiple functions. However, human meddling substantially degrades the functions of rivers and constitutes a threat to river health. Therefore, it is vital to assess and maintain river function. This study used the Xiaoqing River in Shandong Province, China, as a case study and established a multilayered multifunctional river evaluation indicator system consisting of environmental function, ecological function, social function, and economic function. The weights of indicators were calculated using the analytic hierarchy process (AHP) and the entropy method. Furthermore, a fuzzy comprehensive evaluation model based on the Cauchy distribution function was developed to assess the operation status of each function in each river segment. The results of the indicator and criterion layers in different river sections varied. The multifunctionality of the river decreased from upstream to downstream. The Jinan section was the most multifunctional, followed by the Binzhou, Zibo, and Dongying sections, and finally the Weifang section. Through additional analysis, this study determined the constraint indicators and functions of each river section. Overall, the results reveal that the idea of a “multifunctional river” can advance the theoretical understanding of a river’s function, and the fuzzy comprehensive evaluation model is demonstrated to provide fresh perspectives for evaluating river function.
Differential Repeated Sprinting Training in Youth Basketball Players: An Analysis of Effects According to Maturity Status
Authors: Arede Jorge , Fernandes John F. T. , Schöllhorn Wolfgang I. , Leite Nuno
Published: September 2022

The differential learning approach, which includes fluctuations that occur without movement repetitions and without corrections has received growing interest in the skill acquisition field. This study aimed to determine the effects of a 9-week training intervention involving differential repeated sprint training on a series of physical tests in youth basketball players. A total of 29 participants with different maturity statuses (pre-peak height velocity (PHV), n = 7; mid-PHV, n = 6; post-PHV, n = 16) completed 2 sessions per week of differential repeated sprint training for a period of 9 weeks. Sessions consisted of 2 × 10 repetitions sprints of 20-m whereby participants were instructed to perform various additional fluctuations for each repetition. Before and after the training intervention, participants completed jumping tests (countermovement jump (CMJ), single-leg CMJs, the modified 505 agility test, and straight sprinting tests (0–10 splits time), and maturity status was evaluated as well. Within-group analysis showed improvement in CMJ asymmetries and changes in direction asymmetries and 10-m sprint performance for the pre-, mid-, and post-PHV groups, respectively (p < 0.05), with large to very large effects. Analysis of covariance demonstrated that changes in sprint time in post-PHV players were greater than in the pre- and mid-PHV groups (p < 0.05), with moderate effect. Adding random fluctuations during repeated sprint training appear to be a suitable and feasible training strategy for maintaining and enhancing physical performance in youth basketball players, irrespective of maturity status. Furthermore, the present findings encourage practitioners to implement the present approach in youth athletes to improve their physical performance, but they should be aware that training response can vary according to maturity status.
Faith-Based Community Members, Family, and COVID-19: The Role of Family Cohesion, Social Support, and Spiritual Support on Quality of Life, Depression, and COVID-19-Prevention Behaviors
Authors: Wright Kevin Bradley , Davidson Mhonde Rochelle
Published: September 2022

This study examined relationships between family cohesion, social support/spiritual support, and quality of life and depression among faith-based community members during the 2020 COVID-19 restrictions. Drawing upon the buffering model of social support and family cohesion as theoretical frameworks, the authors examined these factors in a survey of 551 faith-based community members between March 2020 and June 2020. Family cohesion had a direct and indirect effect (mediated by overall social support and spiritual support on quality of life). Moreover, family cohesion only had a direct effect on depression (e.g., not mediated by overall social support or spiritual support). Greater family cohesion and overall social support were predictive of increased COVID-19-prevention behaviors, while spiritual support was predictive of reduced COVID-19-prevention behaviors.