bluuebunny/crossref_metadata_2025 huggingface dataset:
Human Rights Advocacy in Silicon Valley: Civil Society’s Perceptions of Platform Accountability Efforts and Stakeholder Engagement Processes
Authors: Brown Kayla
Published: September 2020

This research project evaluates civil society’s perceptions of human rights advocacy and stakeholder engagement within the particular context of content governance and platform accountability. It is informed by critical theories of new media and scholarship on social movements and social change. Findings reveal that, within the context of platform accountability and content governance, organized civil society advocates for human rights by applying external pressure through media coverage and by networking directly with platform companies, many of which are hiring dedicated human rights leads and establishing more robust stakeholder engagement or governance processes; corporate interests underpin both strategies, however, the individuals interviewed identified the former as the most effective. Although organized civil society is an important counterweight to corporate power, findings reveal that business interests are still a significant barrier to enacting meaningful social change. Findings also suggest that good faith by and good actors within platform companies are ultimately not enough, reinforcing the important role organized civil society plays in increasing democratic accountability, even as private corporations begin to create processes to govern themselves.
Proteinuria pada Penderita Gagal Ginjal Kronik di Rumah Sakit Martha Friska Multatuli Medan Tahun 2015-2016
Authors: Sijabat Juliana Ruminta , Sartini Sartini , Karim Abdul
Published: September 2020

This study aims to determine the presence or absence of protein in urine in patients with chronic renal failure. From the results of the study it was found that patients with chronic kidney failure in Martha Friska Multatuli General Hospital Medan from the 80 most patients in positive +++. Many patients with chronic kidney failure are male patients (62.5%) compared to female patients (37.5%) and the results of the examination based on age increase with age, ie at the age of 45 years and above. This type of research is descriptive analytic. Data is taken by recording medical record data of patients with chronic kidney failure based on age, sex and year. The study was conducted from January 2015 to December 2016. The place for conducting the research was conducted at Martha Friska Multatuli General Hospital (RSU) Medan. The study sample was urine samples from all patients with chronic renal failure. It can be concluded that the picture of protein in urine in patients with chronic kidney failure increases every year.
Isolasi Bakteri Asam Laktat (BAL) dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) dan Kemampuannya Dalam Menghambat Staphylococcus aureus dan Shigella sp.
Authors: Bukhori Alpina , Sartini Sartini , Rahmiati Rahmiati
Published: September 2020

Lactic acid bakteria have characteristics that are able to ferment sugars or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this research was to isolates the lactic acid bacteria from the digestive tract of tilapia fish and to investigate the inhibitory power produced by lactic acid bacteria in inhibiting the growth of Staphylococcus aureus and Shigella sp. Isolation of lactic acid bacteria was done by using MRSA medium and to see the ability of lactic acid bacteria isolates in inhibiting the growth of Staphylococcus aureus and Shigella sp then used disc diffusion method. The isolates obtained were then characterized by morphology and biochemistry. From the research obtained two lactic acid bacteria that is sp1 and sp2. Lactic acid bacteria sp1 and sp2 are able to inhibit Staphylococcus aureus and Shigella sp with the biggest inhibition to Staphylococcus aureus by sp2 of 8.75 mm and the biggest inhibition of Shigella sp is shown by sp2 that is equal to 7.16 mm. Based on the results of isolation and characterization done in this research can be concluded that lactic acid bacteria of the digestive tract of fish tilapia sp1 and sp2 capable in inhibiting Staphylococcus aureus with a the number of 8.75 mm and Shigella sp with a of 7.16 mm.
Isolasi dan Identifikasi Bakteri Endofit Pada Akar Cabai (Capsicum annuum L.) Untuk Menghambat Pertumbuhan Jamur (Fusarium oxysporum)
Authors: Fahdila Shela , Susilo Ferdinand , Karim Abdul
Published: September 2020

Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari akar cabai merah Capsicum annuum dan mengetahui kemampuannya dalam menghambat pertumbuhan jamur patogen Fusarium oxysporum. Penelitian ini menggunakan metode deskriptif berskala laboratorium. Hasil isolasi dari akar cabai merah Capsicum annuum ditemukan dua koloni isolat yang berbeda yang diberi dengan kode isolat A1 dan A2. Kedua isolat bakteri endofit diduga merupakan genus Bacillus. Hasil daya antagonis kedua isolat menyatakan bahwa bakteri endofit tersebut mampu menghambat pertumbuhan jamur patogen Fusarium oxysporum, hal ini dapat dilihat dari diameter zona hambat yang terbentuk.
KERAGAMAN JENIS BUAH DURIAN DI DESA NAMOTONGAN KECAMATAN KUTAMBARU KABUPATEN LANGKAT
Authors: Rahayu Winda , Nasution Jamilah , Kardhinata Emmy Harso
Published: September 2020

Keragaman jenis buah durian di Desa Namotongan Kecamatan Kutambaru Kabupaten Langkat. Tanaman durian pada umumnya tumbuh di daerah beriklim tropis. Penelitian ini bertujuan untuk mengetahui keragaman jenis buah durian yang tumbuh di Desa Namotongan Kecamatan Kutambaru Kabupaten Langkat. Metode yang digunakan dalam penelitian ini adalah deskriptif dengan teknik eksplorasi dan dokumentasi. Dari hasil penelitian ini diperoleh 8 jenis buah yaitu durian jantung, durian belimbing, durian bakul, durian bangkok, durian emas, durian rambe, durian tembaga dan durian susu. Buah durian diperoleh total 35 frekuensi yang terdiri dari 50 % frekuensi buah duria jantung, 62,5 % frekuensi buah durian belimbing, 62,5 % frekuensi buah durian bakul, 62,5 % frekuensi buah durian bangkok, 25 % frekuensi buah durian emas, 75 % frekuensi buah durian rambe, 50 % frekuensi buah durian tembaga dan 50 % frekuensi buah durian susu.
Analisis Pemasaran Gabah (Studi Kasus: Desa Serdang, Kecamatan Beringin, Kabupaten Deli Serdang)
Authors: Sitorus Sri Leli , Saleh Khairul , Lubis Mitra Musika
Published: September 2020

This study aimed to determine the grain marketing channels in Desa Serdang, Kecamatan Beringin, Deli Serdang and to determine the efficiency of grain marketing in Serdang Beringin Subdistrict, Deli Serdang regency. Farmers sampling was conducted using proportionate stratified random sampling 53 farmers. While traders Sampel taken by the census method that was as much as three merchants and traders to take as much refinery in three traders with   purposive sampling method.  Beringin the total number of samples in this study was 59 samples. The results showed that there were two channels of grain marketing research location that was, the first channel starts from farmers to traders refinery in District Beringin. The second channel starts from farmers, collectors next to the refinery merchant Beringin Subdistrict. The marketing margin was Rp. 4.500 contained in the channel I and the channel more efficient contained in the channel II with the value of an efficiency of 12,61%.
Aplikasi biochar cangkang kernel kelapa sawit dan limbah baglog jamur terhadap pertumbuhan dan produksi padi beras merah pada pertanaman karet
Authors: Hasibuan Jason , Panggabean Ellen Lumisar , Hutapea Sumihar
Published: September 2020

This research was aiming to find out whether the application of biochar palm kernel shell and mushroom baglog waste. The research was conducted by using the Factorial Randomized Group Design (RAK) with 2 treatment factors. The first factor was the biochar of the palm kernel shell  and The second factor was the mushroom baglog waste which. The result of this research showed that the treatment of  giving biochar to brown rice in rubber plantation had a real effect on the growth of the plant height in the age of 6 MSPT and the weight of production of sample crop and didn’t have a real effect on the production weight per plot, number of tillers, number of panicles of sample crop and the amount of 1000 grains of brown rice. The treatment of giving oyster mushroom baglog tp brown rice in rubber plantation had a real effect on the production of the rice per plot, and the production weight of sample crop and didn’t have a real effect on the growth of the number of the tillers, number of panicles of sample crops, and amount of 1000 grains of brown rice. The combination of both  factors  didn’t  have  a  real  effect  on  the  growth  and  the production of brown rice.
Respon Pertumbuhan Dan Produksi Beberapa Varietas Jamur Tiram Pada Kombinasi Media Serbuk Limbah Pelepah Kelapa Sawit Dan Serbuk Gergaji
Authors: Bate’e Meidaya , Panggabean Ellen Lumisar , Mardiana Siti
Published: September 2020

The demand of oyster mushroom has increased. Hence the production should be improved as well. The purpose of research is to know the growth response and production of several varieties of oyster mushroom on the powder media of palm stem waste and sawdust. Research conducted in Sumatra mushroom oyster cultivation. The design used in this study is a Complete Random Design (CRD) factorial that is of 2 factors. The first factor is the composition of the planting media of palm oil and sawdust was conducted with 2 repeats. The Parameter of observation on the study is the percentage of baglogs covered by mycelium (%), the growth of mycelium on the substrate (cm), the age of the body of the fruit (DAI), Number of fruit body (fruit), diameter of the hood (cm2) The stem length of the fruit body (cm) and the wet Harvest weight (g). The results showed that the best treatment for the growth of mycelium and the age of fruit body in total saw dust amd and varieties jamur tiram 58.38 days formation of the first fruit body.
Respon Pertumbuhan, Produksi Dan Persentase Serangan Penyakit Pada Tanaman Bawang Merah (Allium Ascalonicum L.) Yang Di Beri 3 Jenis Kompos Kulit Buah Dan Poc Kubis
Authors: Fernando Roy , Indrawati Asmah , Azwana Azwana
Published: September 2020

Onion is one of predominant flavor in Indonesia culinary which its quantity should be increased contantly. The aim of research was to invesitigate the respond of onion growth toward the treatment combination between POC cabbage and Tree types of fruit skin. This research was performed in a Factorial Randomized Block Design (RBD), which consists of 2 treatment factors, namely: 1) compost 2) liquid organic fertilizer of cabbage waste. The parameters observed in this study were plant height, number of leaves, number of tubers per sample plant, production per sample, production per plot and percentage of plant disease attack. As for the results obtained from this study, namely: 1) Giving various types of fruit skin compost did not significantly affect height, number of leaves, number of tubers, production per sample plant and production per plot of onion plants; 2) liquid organic fertilizer cabbage application has no significant effect on height, number of leaves, number of tubers and production per sample plant, but has a significant effect on production per plot of onion plant; and 3) Combination between the fruits compost and the liquid organic fertilizer that is not real influence on the plant height, the number of leaves, the number of bulbs and production per crop samples, but the effect is very real towards production per plot of the anion plants
Identifikasi Potensi Swasembada Beras di Sumatera Utara
Authors: Fadly Ridwan , Lubis Yusniar , Saragih Faoeza Hafiz
Published: September 2020

This study aims to determine the potential of districts in North Sumatra Province in an effort to increase rice production in North Sumatra Province. The type of data used in this study is secondary data. The basic method used is descriptive analysis research method. The analytical tool used is Shift Share Analysis (SSA). The results of this study indicate; 1) districts that have potential harvested area in North Sumatra Province are Nias Barat district, Mandailing Natal District, South Tapanuli District, North Padang Lawas Regency, Central Tapanuli Regency, South Nias Regency, Padang Sidempuan Regency, Gunung Sitoli Regency, Tapanuli Regency North, Simalungun Regency, Binjai Regency, Labuhan Batu Regency, Langkat Regency; 2) Districts that have rice production potential in North Sumatra Province are Nias Regency, West Nias Regency, Mandailing Natal Regency, Padang Lawas Utara Regency, South Nias Regency, Padang Sidempuan District Gunung Sitoli, Tapanuli Utara Regency, Toba Samosir Regency, Regency Simalungun, Karo Regency, Batu Bara Regency, Asahan Regency, Binjai Regency; 3) Districts in North Sumatra Province which are the most potential areas, in the harvest area is North Padang Lawas Regency and the most potential area in rice production is Simalungun Regency
Sistem Pengelolaan Arsip pada Biro Sekretariat Dan Umum Kantor Lembaga Pendidikan Perkebunan Medan
Authors: Lestari Ayu , Muda Indra , Dewi Rosmala
Published: September 2020

The purpose of archiving is to find archives easily and quickly if at any time it is needed again. This research was conducted at the Medan Plantation Education Institute office. The formulation of the problem in this study is how the file management system, and the obstacles in managing the records of the Medan Plantation Education Office. This research uses qualitative research that describes the records management system. Informants in this study were employees of the Medan Plantation Education Office. Based on the results of the study, the archive management system carried out at the Medan Plantation Education Office office covers how the implementation process starts from recording, storing, maintaining, structuring and transferring, to destroying the archive. The obstacles in managing archives are the absence of a special room for storing archives and also the absence of special staff to manage the archives. Efforts made in managing archives are to add facilities and infrastructure of archives to make it easier and orderly, making proposals to provide special staff to handle archives, as well as implementing an automation system in records management, namely electronic processing using computer facilities and other information technology.
Strategi Pemasaran Brothers Café dalam Membangun Brand Image Bagi Pengunjung Di Kota Medan
Authors: Hidayat Putra Rahmat , Ritonga Syafruddin , Suharyanto Agung
Published: September 2020

The purpose of this study was to determine the marketing communication strategy undertaken by Brothers Cafe in building brand image for visitors in Medan and its supporting factors and obstacles. The research method is a descriptive qualitative data collection technique with methods of observation, interviews, library research, and documentation. The results of marketing communication strategy research conducted by Brothers Cafe to build brand image, present European-style concepts with a western theme, present a unique menu with an international standard, conduct event programs on holidays, utilize social media such as Instagram and others, work with communities and companies Other companies and factors that support marketing communication strategies in building brand image for visitors in Medan are promotional activities in building brand image for visitors, including locations in office centers, and office buildings, providing facilities, comfortable decoration, presence Special discount for customers who become members. The inhibiting factors of the Brothers cafe marketing communication strategy in building brand image for visitors in Medan are issues such as adulteration, nutritional problems, halal and haram, everyone's tastes are completely different, operational costs rise at any time, sufficient business competition strict. The conclusion obtained by the marketing communication strategy that Brothers cafe did in building brand image for visitors in Medan through promotional activities.
Persepsi Pasien Tentang Komunikasi Interpersonal Front Desk dalam Pelayanan Pelanggan di Rumah Sakit Sumatera Eye Center Medan
Authors: Baiti Nurul Tiara , Hasibuan Effiati Juliana , Suharyanto Agung
Published: September 2020

Penelitian ini bertujuan untuk mengetahui persepsi pasien tentang komunikasi interpersonal front desk di Rumah Sakit Sumatera Eye Center Medan. Penelitian ini menggunakan metode penelitian deskriptif kualitatif dengan menjadikan 5 (lima) orang pasien Rumah Sakit Sumatera Eye Center sebagai informan. Berdasarkan penelitian yang dilakukan, maka diperoleh hasil bahwa para pasien yang berobat di Sumatera eye center, mendapatkan pelayanan yang baik dan ramah. Komunikasi interpersonal yang diterapkan oleh front desk di Rumah Sakit Sumatra Eye Centre Medan tidak hanya besifat verbal tetapi juga non verbal yang meliputi pesan kinesik dan paralinguistik. Komunikasi non verbal yang dipergunakan untuk memperkuat pesan-pesan verbal sehingga komunikasi lebih kaya. Persepsi pasien tentang komunikasi interpersonal yang dilakukan oleh front desk Rumah Sakit Sumatra Eye Centre terbilang cukup baik. Walaupun ditemukan beberapa hal yang dianggap pasien belum atau kurang maksimal dan pasien merasa puas dengan pelayanan yang diberikan oleh front desk yang selalu bisa memberikan informasi dengan baik. Disimpulkan bahwa komunikasi interpersonal diterapkan oleh front desk di Rumah Sakit Sumatra Eye Centre Medan besifat verbal dan non verbal yang meliputi pesan kinesik dan paralinguistik, ditemukan beberapa hal yang belum atau kurang maksimal dan pasien merasa puas dengan pelayanan oleh diberikan front desk yang bisa memberikan informasi dengan baik.
Pengembangan Rubrik Penskoran pada Asesmen Otentik untuk Materi Volume dan Luas Balok
Authors: Jonan Yunita Rochmawati
Published: September 2020

ABSTRAK

Tujuan penelitian pengembangan ini adalah menghasilkan sebuah rubrik penskoran pada asesmen otentikuntuk materi volume dan luas balok yang dapat digunakan oleh guru dan siswa. Rubrik ini dibuat sesuai dengan standar penilaian dalam kurikulum 2013. Penelitian pengembangan (R&D) ini menggunakan model Borg and Gall yang terdiri dari lima tahap yaitu penelitian dan pengumpulan data awal, perencanaan, pengembangan format produk, uji coba skala kecil, revisi akhir dan penyempurnaan produk. Hasil penelitian pengembangan ini berupa rubrik penyekoran holistik dan analitik dengan nilai sangat valid sebesar 94%. Analisis dari penelitian ini adalah angket kebutuhan bagi guru dan siswa sedangkan uji coba dilakukan melalui perorangan, kelompok kecil dan kelompok besar. Sedangkan kelayakan rubrik penskoran dari penilaian oleh ahli dengan menggunakan angket uji validitas materi, hasil belajar siswa, respon siswa serta tanggapan guru pengajar. Hasil penelitian ini menunjukkan bahwa rubrik penskoran ini efektif dalam membantu memperbaiki hasil ulangan dan cukup praktis serta dapat dipergunakan untuk memberikan penilaian hasil belajar yang sesuai dengan kurikulum 2013.

Kata kunci: rubrik, penskoran, asesmen otentik.

 

ABSTRACT

The purpose of this development research is to produce a scoring rubric on an authenticity assessment for the volume and area materials that can be used by teachers and students. The rubric is made in based on the assessment standards in the 2013 curriculum. This development research (R&D) uses the Borg and Gall model consisting of five stages of preliminary data research and collection, planning, product format development, small-scale trials, final revisions and product enhancements. The results of this development research include holistic and analytic scoring rubric with a very valid score of 94%. The rubric is analyzed fromquestionnaire for teachers and students, while trials are conducted through individuals, small groups and large groups. The feasibility of the scoring rubric from assessment by members by using the material validity test questionnaire, student learning results, student response and teacher responses. The results of this study show that the scoring rubric is effective in helping to improve the test results and is practical and can be used to provide assessment of learning outcomes in accordance with the 2013 curriculum.

Keywords: rubric, scoring, authentic assessment.
The effect of APOL1 risk variants on emergent outcomes in kidney disorders: A meta-analysis of individual participant data
Authors: Amiri Fateme Shamekhi
Published: September 2020

Abstract. Kidney diseases associated with APOL1 polymorphisms are human immunodeficiency virus-associated nephropathy, idiopathic focal segmental glomerulosclerosis, hypertension-attributed chronic kidney disease, lupus nephritis and sickle cell nephropathy. This research aimed to investigate the risk of genetic variants on disease contribution.

Methods. In this individual participant data meta-analysis, eighteen patients with kidney dysfunction and at risk of APOL1 genotype were investigated. Clinical features, laboratory data at initial presentation, management and outcomes were collected. The paper has written based on searching PubMed Central and Google Scholar to identify potentially relevant articles. Median, percentage, mean ± standard deviation (SD), two-tailed t and chi-square tests were used for statistical analyses. Moreover, relative risk, odds ratio for statistical analyses were used.

Results: The average age of patients at the time of diagnosis in APOL1-associated kidney disorders was 41.09 ± 20.63 years (ranging from 8 years to 70 years). Relative risk for kidney failure and persistent hemodialysis therapy in APOL1-associated nephropathy patients with renal risk variants (RRVs) were assessed 1.13 and odds ratio of 1.5 with 95% CI of 0.08-26.86 and the value of 0.0764 by chi-square test but there was no significant statistical result in this research (p-value of 0.782). The relative risk for patients of allograft failure with RRVs was assessed 1,0 odds ratio of 1,0 95% CI of 0.06-15.99 and p-value of 0.81.

Conclusion: The present study revealed the risk and odds of APOL1 gene effect on the onset of kidney failure with replacement therapy in patients at risk of APOL1 genotype but results were not significant statistically. Future clinical research is required for investigating APOL1 gene effect on non-African ancestry.
COVID-19 and kidney
Authors: Kolesnyk M.
Published: September 2020

At the beginning of COVID-19 pandemic attention of healthcare professionals and scientists were already drawn to the appearance of markers of pathologic changes of the urinary system in SARS-CoV-2-infected patients, signs of acute kidney disease (AKD) (including acute kidney injury (AKI) or development of AKI in patients with chronic kidney disease (CKD). This necessitates verification of pathologic changes markers of the urinary system in SARS-CoV-2-infected patients and clarification of their nosologic relevance.

The present study aimed to analyze the present information regarding the capacity of SARS-CoV-2 to cause kidney injury (acute kidney disease, including AKI) in patients without such changes before infecting or in patients with CKD, and to verify these changes according to the classification of urinary system disease and Nomenclature for kidney function and disease: report of Kidney Disease: Improving Global Outcomes (KDIGO) Consensus Conference 2020.

The presence of the pathologic changes markers of the urinary system in SARS-CoV-2-infected patients necessitates its verification via the use of tools of diagnostics of urinary system disease.

Infecting with SARS-CoV-2 may cause (isolated) asymptomatic proteinuria, isolated erythrocyturia, hemoglobinuria; AKD (including AKI) in patients without preexisting urinary system injuries, AKI in patients with CKD, and may complicate hemodialysis and peritoneal dialysis. The frequency of AKI and mortality rate in patients with COVID-19 and CKD are much higher than without the last one.

The AKI frequency is higher in ICU (18 – 37,5%) than in patients with moderate or mild COVID-19 (0,5 – 15%).

Patients with all CKD stages with moderate or severe COVID-19 must be admitted to the hospital with further determination by a multidisciplinary team (infectionist, nephrologist, ICU physician, etc, according to the clinical situation) of necessary monitoring and treatment capacity for prevention of AKD progression and life-threatening complications or their adequate therapy.

After confirmation of SARS-CoV-2 absence and release from the hospital, patients with AKD or all stages of CKD should be followed up, and monitoring frequency depends on AKD or CKD stage.
3D printing and urology: Review of the clinical applications
Authors: Wendo K. , Olszewski R.
Published: September 2020

Three-dimensional (3D) printing is a process that translates a 3D virtual model into its physical 3D replica. In medicine, Neurosurgery, Orthopedics and Maxillo-facial surgery were the first specialties to successfully incorporate this technology in their clinical routine, as an aid to surgical interventions.

The study aimed to provide a clear overview of the potential areas of applications of 3D printing (3DP) for management of renal diseases, based on a review of the literature.

Method. We carried out a review of the literature according to PRISMA recommendations. We searched three databases (Medline, Scopus and Cochrane) with two specific queries: one using MeSH-terms and the second one based on free terms, all terms were related to nephrology and three-dimensional printing technology.

Results. 3D-printed models were mostly employed for the management of renal tumors and lithiasis. They provided enhanced visualization of structures and the possibility to perform procedures rehearsals which seemed to improve surgical procedures. Models were also reported to positively impact patients’ understanding of their condition and the interventions. Trainees and experienced urologists also benefited from the supportive role of 3D-printed models and reported improved confidence and efficiency. Rare reports discussed their use for kidney transplantation, ureteropelvic junction obstruction syndrome treatment, nuclear medicine or cultural issues. Due to a meager data amount and heterogeneity of studies, no advanced statistical analysis was possible.

Conclusion. 3D-printed models of renal anatomical structures are feasible and are valuable tools to support renal disease management, and for educational purposes.
Highlighting the Role of Cdk6 Associated MicroRNAs in Cancer Treatment Using In Silico Approaches
Authors: Batool Sidra , Hasan Mahmood Muhammad Sibte , Furqan Tiyyaba , Batool Sidra
Published: September 2020

MicroRNAs (miRNAs) are small non-coding RNA’s that controls the regulation of a gene. Due to the over
expression or under expression of miRNAs it leads to cause tumor or any other type of cancers such as,
melanoma, lymphoma, cardiovascular issue, breast cancer etc. So, miRNAs can be used as a drug target for
cancer therapy. This study aimed to check binding cavities of microRNA's involved in regulation of CDK6
protein. There are 23 different families of miRNAs that are involved in regulation of CDK6. Each family
has one or more miRNAs. All these miRNAs are involved in the up regulation or downregulation of a gene,
which lead to different type of cancers. All miRNAs of each family docked with mRNA CDK6 protein.
After performing in silico analysis of binding interactions of mRNA with miRNAs the results were further
refined by their comparison with information regarding their energies, interaction of the mRNA and
miRNAs. The results show that all miRNAs lie in Protein Kinase domain, but the residues that lie is different
within the families and across the families.
p38 MAP Kinase-Mediated Odontogenic Differentiation of Dental Pulp Stem Cells
Authors: Chung Seung , Cheng William , Valverde Yessenia , Lee Nam-Seob , Marzban Hassan , Chung Seung , Alapati SB
Published: September 2020

The multipotent nature of dental pulp stem cells (DPSCs) promises regenerative endodontic potentials.
Alterations in microenvironment have been shown to control the differentiation phenotypes of DPSCs.
Understanding the biological mechanisms and finding the optimal DPSC differentiation protocols are
crucial for successful DPSC engineering strategies in pulp and dentin healing. The aim of this study is to
identify the role of p38 mitogen-activated protein kinase (p38) under normal and oxygen-deprived
conditions (2%) to reveal its effect on odontogenic DPSC differentiation. Human DPSCs were isolated from
healthy molars and underwent odontogenic differentiation in regular and osteogenic media treated with
SB203580, a p38 inhibitor, for 72 hours, and then swapped with osteogenic media for 21 days under hypoxic
condition. Immunochemistry and PCR analysis for the various odontogenic differentiation genes and
proteins were performed. Our PCR data demonstrate that p38 inhibition resulted in a significant
upregulation in odontogenic gene expressions such as DMP-1, DSPP, RUNX, and OSX in normal
conditions. Under hypoxia, this effect was reversed. These results were further supported by DSPP
immunohistochemistry. The DSPP expression under hypoxia was significantly weaker compared to the
control. Our results indicate that p38 represents a negative regulator of the odontogenic DPSC
differentiation in normoxia. Under hypoxia, p38 exerts a positive function of DPSC differentiation. Taken
together, we identified the p38 and oxygen level as crucial factors to control odontogenic DPSC
differentiation providing their essential roles in designing for successful pulp-dentin complex engineering
strategies.
Longitudinal Melanonychia Treatment with Picosecond Laser for Differential Diagnosis
Authors: Fritz Klaus , Fritz Klaus , Salavastru Carmen
Published: September 2020

Longitudinal melanonychia can have multiple causes, among them subungual melanoma. Clearing the pigmentation with pigment specific lasers, especially picosecond laser with less pain can be a comfortable and reliable alternative to ablation in the differential diagnosis.
Comparative Pharmacokinetic Analysis of Community Use Naloxone formulations for Acute Treatment of Opioid Overdose
Authors: B. Moss Ronald , Carleton Fiona , P. Lollo Charles , J. Carlo Dennis
Published: September 2020

Recent increases in mortality from overdoses in the US have been primarily driven by deaths due to the more potent synthetic opioids. Naloxone is an effective countermeasure to treat opioid overdose. We compared the pharmacokinetics of the three available doses of naloxone used to treat prehospital opioid overdoses. Overall, the systemic exposures of 2 mg intramuscular and 4 mg intranasal of naloxone appear to be similar. By comparison, the exposure levels of the 5 mg dose intramuscular naloxone (ZIMHI) appears to be greater and more rapid. These results support the notion that higher doses of naloxone result in greater bioavailability which may be required for reversal due to the more potent synthetic opioids, such as fentanyl.
A Wolff- Parkinson- White Surprise Case
Authors: Vargas William
Published: September 2020

37-year-old-gentleman, who was presented in three occasional times to emergency department for a fast, precordial, and rhythmic symptomatic palpitations, in which the last episode was with a duration that lasted more than an hour with instability vitally that ended in performing emergency electrical cardioversion. All these episodes were symptomatic and last hospitalization was associated with pre syncopal and syncopal attacks. With evidence of Wolff-Parkinson-White syndrome the patient was brought to Electrophysiology Lab to EP study and ablation.
Number of Spinal-Convex Polyominoes
Authors: Sabri Mustafa  A. , F. Mohomme Eman
Published: September 2020

In his paper we describe a restricted class of polyominoes called spinal-convex polyominoes. Spinal-convex polyominoes created by two columns such that column 1 (respectively, column2) with at most two set columns sequence of adjacent ominoes and column 2 (respectively, column1) with at least one set column sequence of adjacent ominoes. In addition, this study reveals new combinatorial method of enumerating spinal-convex polyominoes. Keywordsâ€”: Polyominoes, Spinal-convex, Set column sequence, enumerating.
Study of the stability of the emulsion of ultramicroheterogeneous flotation reagents obtained by the method of ultrasonic dispersion
Authors:   Institute of Metallurgy and Ore Benefiaction JSC, Satbayev University, Almaty, Kazakhstan, Yessengaziyev A.M. , Barmenshinova M.B. ,   Institute of Metallurgy and Ore Benefiaction JSC, Satbayev University, Almaty, Kazakhstan, Bilyalova S.M. ,   Institute of Metallurgy and Ore Benefiaction JSC, Satbayev University, Almaty, Kazakhstan, Mukhanova A.A. ,   Institute of Metallurgy and Ore Benefiaction JSC, Satbayev University, Almaty, Kazakhstan, Muhamedilova А.М. ,   Institute of Metallurgy and Ore Benefiaction JSC, Satbayev University, Almaty, Kazakhstan
Published: September 2020

The results of studies of the stability of the microemulsion of an ultramicroheterogeneous flotation reagent obtained from a mixture of oil from the Zhanazhol field and butyl xanthate (BX) in the following ratios are presented: oil: BX = 1: 1; oil: BX = 2: 1; oil: BX = 4: 1. It was found that the microemulsion has the greatest stability when the ratio of oil: BX = 4: 1, which was 99.3% after 120 hours of exposure. The supposed emulsifying effect of butyl xanthate in symbiosis with natural emulsifiers of the oil itself over newly formed globules of ultrasonic (US) dispersion was found. At an ultrasonic treatment power of 250 W for 10 min, a microemulsion with an average globule size of 123.4 nm was obtained. It is shown that an increase in the duration of ultrasonic treatment for more than 10 minutes does not have a significant effect on the stability of the obtained microemulsion of an ultramicroheterogeneous reagent
Development of superior somaclones of aromatic local cultivar of rice (Oryza sativa L.)
Authors: Roy Bidhan
Published: September 2020

The traditional local aromatic rice cultivars are poor combiners. Here we report development of superior somaclones plants through tissue culture. The somaclones, TC 4/8 had the maximum yield followed by TC 5-1, TC 4/4, TC 4/5, and TC 4/7. Yield increase of TC 4/8, TC-5-1 was 54.75% and 50.33% more over the yield of parental cultivar, respectively. The plant tissue culture thus may be recommended to create genetic variability in rice as a trustworthy biotechnological tool.
Osmotic stress induced root growth and regulation of root development related genes in progenitors of wheat
Authors: Dalal Monika , Tiwari Sneha , . Vinod
Published: September 2020

Maintenance of root growth during water deficit can significantly contribute to yield stability. In this study, total nine accessions, three each of Triticum monococcum, Aegilops speltoides and Aegilops tauschii were analysed for root traits under two levels of osmotic stress at seedling stage. T. monococcum accession (A2) showed highest increase in total root growth while 17% and 34% increase in primary root length was recorded in T. monococcum (A2) and Ae. speltoides (B3) under osmotic stress. Expression of BREVIS RADIX (BRX) and NAM/ATAF/CUC 1(NAC1) was analysed in three diploid accessions with contrasting root phenotype. BREVIS RADIX, a transcription factor modulating root length was up regulated in T. monococcum A2 accession while NAC1 expression was up regulated in three of the accessions under osmotic stress. The accessions and genes analysed in the study can be good resource to explore the molecular-genetic mechanism of root growth under stress.
An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells
Authors: Zhang Yu-Miao , Wang Jun , Wu Tao
Published: September 2020

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.
Identification of novel haplotype of a cyst nematode resistance gene, GmSNAP18 in soybean [Glycine max (L.) Merr.]
Authors: Choi Ik-Young , Basnet Prakash , Yoo Hana , Roy Neha Samir , Ramekar Rahul Vasudeo , Park Kyong-Cheul
Published: September 2020

Soybean cyst nematode (SCN) is one of the most damaging pest of soybean. Discovery and characterization of the genes involved in SCN resistance are important in soybean breeding. Soluble NSF attachment protein (SNAP) genes are related to SCN resistance in soybean. SNAP genes include five gene families, and 2 haplotypes of exons 6 and 9 of SNAP18 are considered resistant to the SCN. In present study the haplotypes of GmSNAP18 were surveyed and chacterized in a total of 60 diverse soybean genotypes including Korean cultivars, landraces, and wild-types. The target region of exons 6 and 9 in GmSNAP18 region was amplified and sequenced to examine nucleotide variation. Characterization of 5 haplotypes identified in present study for the GmSNAP18 gene revealed two haplotypes as resistant, 1 as susceptible and two as novel. A total of twelve genotypes showed resistant haplotypes, and 45 cultivars were found susceptible. Interestingly, the two novel haplotypes were present in 3 soybean lines. The information provided here about the haplotypic variation of GmSNAP18 gene can be further explored for soybean breeding to develop resistant varieties.
Genetic diversity, association and principle component analyses for agronomical and quality traits in genomic selection training population of groundnut (Arachis hypogaea L.)
Authors: Viswanatha K. P. , Patil Rajendragouda , Upadhyaya H. D. , Khan Hasan , Gururaj S. , . Somasekhar
Published: September 2020

Groundnut (Arachis hypogaea L.) is the world’s third important source of oil. A Genomic Selection Training Population (GSTP) comprising 340 genotypes was evaluated over four locations for two seasons to study genetic variability and association among agronomical and quality traits. GSTP exhibited significant variation among the genotypes, seasons and G x E interaction. Moderate to high magnitude of genotypic coefficient of variation and phenotypic coefficient of variation coupled with high heritability was observed for most quality traits. The first 7 components of PCA analysis contributed more than 75% cumulative variability. The training population grouped into three clusters in both the seasons. The number of pods/plant, pod and seed yield/plant were significantly and positively associated with each other, while test weight had negative association with number of pods/plant and had positive association with pod and seed yield during rainy season of 2015. The significant positive correlation was also observed between oil, linoleic acid and stearic acid; similarly, linoleic acid had positive association with stearic acid and palmitic acid. Significant negative correlation was observed between oil and protein content, oleic acid and linoleic acid content. The superior genotypes, namely, ICG 5221, ICGV 01393, ICGV 07220, ICGV 97120, ICGV 06420, ICG 9507, ICGV 06188 and ICGV 00440 were best performer for yield, yield components and nutritional quality traits while ICG 2381 and ICG 5221 recorded a better performance for oil content. The rainy season was found to have an advantage for vegetative, physiological growth, oil content, oleic and oleic to linoleic ratio while the post-rainy season led to forced pod filling and reduced maturity.
Conocimiento tradicional y academia: productores de maguey y mezcal de pequeña escala en las regiones Norte y Centro de Guerrero, México
Authors: Barrientos-Rivera Guillermina , Hernández-Castro Elías , Sampedro-Rosas Ma. Laura , Segura-Pacheco Héctor Ramón
Published: September 2020

El objetivo de la investigación de la que se deriva este artículo fue documentar el conocimiento tradicional de los productores de maguey y mezcal del estado de Guerrero en México: sus prácticas agronómicas, los aspectos ecológicos imbricados en éstas, así como sus usos, costumbres, tradiciones y las alternativas que han instrumentado para rescatar y preservar el conocimiento tradicional; ello con el fin de explorar posibilidades de articulación con los aportes de la investigación científica. El estudio se realizó en la región Centro (cinco localidades de dos municipios) y Norte (tres localidades de dos municipios) del estado de Guerrero entre enero de 2017 y marzo de 2018. Se hicieron entrevistas semiestructuradas a informantes clave (productores de maguey-mezcal), así como recorridos de campo y observación participante. Si bien el maguey es esencial en la economía de muchas familias rurales de estas regiones para la elaboración de mezcal, la actividad agaveramezcalera se encuentra amenazada debido a las condiciones ambientales desfavorables y la sobreexplotación de las otrora abundantes plantas silvestres, por lo que los productores participantes en el estudio consideraron fundamental el desarrollo de actividades conservacionistas del agave entre sus familias para así integrarlas a la dinámica de producción y conservación, no sólo de las plantas, sino de los conocimientos y valores comunitarios que permiten mantener viva la tradición milenaria de producir maguey y mezcal; ámbito donde la comunidad científica puede desempeñar un papel notable en apoyo de este esfuerzo.
Los infinitos tentáculos del neoliberalismo verde
Authors: Vázquez García Verónica
Published: September 2020

Reseña del libro: Naturaleza y neoliberalismo en América Latina (México: Centro Regional de Investigaciones Multidisciplinarias-UNAM, 570 pp).
An Online Linguistic Analyser for Scottish Gaelic
Authors: Boizou Loïc , Lamb William
Published: September 2020

This paper describes the Gaelic Linguistic Analyser, a new resource for the Scottish Gaelic language. The GLA includes a tagger, a lemmatiser and a parser, which were developed largely on the basis of existing resources. This tool is available online as the first component of the Scottish Gaelic Toolkit.
Acute effects of local vibration stretching on ankle range of motion, vertical jump performance and dynamic balance after landing
Authors: Maeda Noriaki , Urabe Yukio , Kotoshiba Somu , Komiya Makoto , Morikawa Masanori , Nishikawa Yuichi , Sasadai Junpei
Published: September 2020

BACKGROUND:

Local vibration (LV) used as part of the warm-up can stimulate a specific body part and muscle group, potentially increasing muscle flexibility and performance. However, the effect of its combination with static stretching (SS) has not been thoroughly examined.

OBJECTIVE:

To elucidate the acute effectiveness of combining LV and SS (V[Formula: see text]S) on the ROM of ankle dorsiflexion, squat jump, counter-movement jump (CMJ) and the dynamic postural stability index (DPSI).

METHODS:

Fifteen healthy men who were regularly involved in recreational sports participated in this study. Static Stretching, V[Formula: see text]S, and non-stretching condition (control) were assigned randomly and the intervention period for each condition was five minutes.

RESULTS:

The dorsiflexion improved significantly in SS and V[Formula: see text]S compared to the control. The CMJ height decreased significantly following SS compared to V[Formula: see text]S and control.

CONCLUSIONS:

This study suggests that V[Formula: see text]S improves ankle dorsiflexion ROM without compromising jump performance. Local vibration device could be an effective element in warming up but further research is warranted.
Effects of 24 weeks strength training with and without unstable devices on strength, flexibility and quality of life in older women: A secondary analysis from randomized controlled trial
Authors: Pirauá André Luiz Torres , de Oliveira Valéria Mayaly Alves , Cavalcante Bruno Remígio , Beltrão Natália Barros , Batista Gabriel de Amorim , Pitangui Ana Carolina Rodarti , Araújo Rodrigo Cappato de
Published: September 2020

BACKGROUND:

Combining strength training (ST) with unstable surfaces (US) is a promising complementary approach to traditional ST to optimize muscle strength and balance in different populations.

OBJECTIVE:

To evaluate the effects of traditional ST and ST+US on grip strength, flexibility and quality of life in older women.

METHODS:

Fifty-eight older women were randomly assigned into ST ([Formula: see text] 22), ST[Formula: see text]US ([Formula: see text] 22) or control ([Formula: see text] 14) group. Exercise groups performed whole-body, moderate-intensity strength exercises, thrice a week during 24-weeks. ST[Formula: see text]US participants performed the same exercise prescription progressively adding unstable surfaces and devices. Dependent variables (grip strength, flexibility and health-related quality of life) were measured at baseline, 12-weeks and after 24-weeks of intervention.

RESULTS:

At completion of 24-weeks, compared with control group, traditional ST promote flexibility gains [SRT [Formula: see text] [Formula: see text] 5.42 cm (95% CI [Formula: see text] 1.01 to 9.83)]. Both training regimes improved quality of life [ST [Formula: see text] [Formula: see text]9.50 (95% CI [Formula: see text] 1.80 to 17.20); ST[Formula: see text]US [Formula: see text] [Formula: see text] 15.23 (95%CI [Formula: see text] 7.37 to 23.08). No significant between-group difference was observed for grip strength at completion of the intervention.

CONCLUSION:

Traditional strength exercises were effective to improve flexibility and health-related quality of life among healthy older women. Combining unstable devices with traditional exercise did not provide additional gains in order to promote flexibility and quality of life.
Photovoice as a Participatory Research Tool in Amyotrophic Lateral Sclerosis
Authors: Gunton Adrianna , Hansen Gregory , Schellenberg Kerri Lynn
Published: September 2020

Background:

Photovoice is a qualitative research tool increasingly utilized in the healthcare field to understand the illness experience from the patient and caregiver perspective. This is the first study to evaluate photovoice in the context of amyotrophic lateral sclerosis (ALS).

Objective:

A patient and caregiver centered research tool was utilized to gain a greater understanding of challenges faced when living with ALS.

Methods:

Eight patients and three corresponding caregivers participating by taking photographs, writing descriptive text, and participating in individual and group interviews. Inductive thematic analysis was employed to uncover recurring themes.

Results:

Five main themes were identified; 1) facing the diagnosis, 2) loss of function, 3) isolation, 4) health system challenges, and 5) hope. Despite the devasting impact of ALS, the majority of participants reported a surprising amount of positivity in the face of receiving this difficult diagnosis, and demonstrated incredible creativity and adaptability to meet the ensuing loss of function. However, patients and caregivers discussed feelings of isolation and health care system challenges. The importance of hope was a strong and recurring theme.

Conclusions:

The photovoice research tool demonstrates the profound resilience of these participants, and challenges the medical community to find ways of fostering positivity and hope throughout the ALS disease course. Further clinic and community resources, education, and supports are needed to combat the sense of isolation and health care system challenges experienced by patients and their caregivers.