bluuebunny/crossref_metadata_2025 huggingface dataset:
Day-to-day spontaneous social behaviours is quantitatively and qualitatively affected in a 16p11.2 deletion mouse model
Authors: Rusu Anna , Chevalier Claire , de Chaumont Fabrice , Nalesso Valérie , Brault Véronique , Hérault Yann , Ey Elodie
Published: December 2023

Background

Autism spectrum disorders affect more than 1% of the population, impairing social communication and increasing stereotyped behaviours. A micro-deletion of the 16p11.2 BP4-BP5 chromosomic region has been identified in 1% of patients also displaying intellectual disabilities. In mouse models generated to understand the mechanisms of this deletion, learning and memory deficits were pervasive in most genetic backgrounds, while social communication deficits were only detected in some models.

Methods

To complement previous studies, we itemized the social deficits in the mouse model of 16p11.2 deletion on a hybrid C57BL/6N × C3H.Pde6b+ genetic background. We examined whether behavioural deficits were visible over long-term observation periods lasting several days and nights, to parallel everyday-life assessment of patients. We recorded the individual and social behaviours of mice carrying a heterozygous deletion of the homologous 16p11.2 chromosomic region (hereafter Del/+) and their wild-type littermates from both sexes over two or three consecutive nights during social interactions of familiar mixed-genotype quartets of males and of females, and of same-genotype unfamiliar female pairs.

Results

We observed that Del/+ mice of both sexes increased significantly their locomotor activity compared to wild-type littermates. In the social domain, Del/+ mice of both sexes displayed widespread deficits, even more so in males than in females in quartets of familiar individuals. In pairs, significant perturbations of the organisation of the social communication and behaviours appeared in Del/+ females.

Discussion

Altogether, this suggests that, over long recording periods, the phenotype of the 16p11.2 Del/+ mice was differently affected in the locomotor activity and the social domains and between the two sexes. These findings confirm the importance of testing models in long-term conditions to provide a comprehensive view of their phenotype that will refine the study of cellular and molecular mechanisms and complement pre-clinical targeted therapeutic trials.
Detrimental effect of anemia after mechanical thrombectomy on functional outcome in patients with ischemic stroke
Authors: Inui Ryoma , Koge Junpei , Tanaka Kanta , Yoshimoto Takeshi , Shiozawa Masayuki , Abe Soichiro , Ishiyama Hiroyuki , Imamura Hirotoshi , Nakahara Jin , Kataoka Hiroharu , Ihara Masafumi , Toyoda Kazunori , Koga Masatoshi
Published: December 2023

Background

Anemia can occur due to an aspiration maneuver of blood with thrombi during mechanical thrombectomy (MT) for stroke. However, the association between postoperative anemia and stroke outcomes is unknown.

Methods

In a registry-based hospital cohort, consecutive patients with acute ischemic stroke who underwent MT were retrospectively recruited. Patients were divided into the following three groups according to their hemoglobin (Hb) concentrations within 24 h after MT; no anemia (Hb concentrations ≥13 g/dL for men and ≥ 12 g/dL for women), mild anemia (Hb concentrations of 11–13 g/dL and 10–12 g/dL, respectively), and moderate-to-severe anemia (Hb concentrations <11 g/dL and < 10 g/dL, respectively). A 3-month modified Rankin Scale score of 0–2 indicated a favorable outcome.

Results

Of 470 patients, 166 were classified into the no anemia group, 168 into the mild anemia group, and 136 into the moderate-to-severe anemia group. Patients in the moderate-to-severe anemia group were older and more commonly had congestive heart failure than those in the other groups. Patients in the moderate-to-severe anemia group also had more device passes than those in the other groups (p < 0.001). However, no difference was observed in the rate of final extended thrombolysis in cerebral infarction ≥2b reperfusion or intracranial hemorrhage among the groups. A favorable outcome was less frequently achieved in the moderate-to-severe anemia group than in the no anemia group (adjusted odds ratio, 0.46; 95% confidence interval, 0.26–0.81) independent of the baseline Hb concentration. A restricted cubic spline model with three knots showed that the adjusted odds ratio for a favorable outcome was lower in patients with lower Hb concentrations within 24 h after MT.

Conclusion

Moderate-to-severe anemia within 24 h after MT is independently associated with a reduced likelihood of a favorable outcome.

Clinical trial registration

https://www.clinicaltrials.gov, NCT02251665.
Endogenous SIRT6 in platelets negatively regulates platelet activation and thrombosis
Authors: Liu Yanli , Wang Tao , Zhou Qilong , Xin Guang , Niu Hai , Li Fan , Wang Yilan , Li Shiyi , Dong Yuman , Zhang Kun , Feng Lijuan , Fu Wei , Zhang Boli , Huang Wen
Published: December 2023

Thromboembolism resulting from platelet dysfunction constitutes a significant contributor to the development of cardiovascular disease. Sirtuin 6 (SIRT6), an essential NAD+-dependent enzyme, has been linked to arterial thrombosis when absent in endothelial cells. In the present study, we have confirmed the presence of SIRT6 protein in anucleated platelets. However, the precise regulatory role of platelet endogenous SIRT6 in platelet activation and thrombotic processes has remained uncertain. Herein, we present compelling evidence demonstrating that platelets isolated from SIRT6-knockout mice (SIRT6−/−) exhibit a notable augmentation in thrombin-induced platelet activation, aggregation, and clot retraction. In contrast, activation of SIRT6 through specific agonist treatment (UBCS039) confers a pronounced protective effect on platelet activation and arterial thrombosis. Moreover, in platelet adoptive transfer experiments between wild-type (WT) and SIRT6−/− mice, the loss of SIRT6 in platelets significantly prolongs the mean thrombus occlusion time in a FeCl3-induced arterial thrombosis mouse model. Mechanistically, we have identified that SIRT6 deficiency in platelets leads to the enhanced expression and release of proprotein convertase subtilisin/kexin type 9 (PCSK9), subsequently activating the platelet activation-associated mitogen-activated protein kinase (MAPK) signaling pathway. These findings collectively unveil a novel protective role of platelet endogenous SIRT6 in platelet activation and thrombosis. This protective effect is, at least in part, attributed to the inhibition of platelet PCSK9 secretion and mitogen-activated protein kinase signaling transduction. Our study provides valuable insights into the intricate interplay between SIRT6 and platelet function, shedding light on potential therapeutic avenues for managing thrombotic disorders.
Cysteine and glycine-rich protein 3 (Crp3) as a critical regulator of elastolysis, inflammation, and smooth muscle cell apoptosis in abdominal aortic aneurysm development
Authors: de Mattos Ana Barbosa Marcondes , Ribeiro-Silva Joao Carlos , Fonseca-Alaniz Miriam Helena , Valadão Iuri Cordeiro , da Silva Erasmo Simão , Krieger Jose Eduardo , Miyakawa Ayumi Aurea
Published: December 2023

Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease for which surgical or endovascular repair are the only currently available therapeutic strategies. The development of AAA involves the breakdown of elastic fibers (elastolysis), infiltration of inflammatory cells, and apoptosis of smooth muscle cells (SMCs). However, the specific regulators governing these responses remain unknown. We previously demonstrated that Cysteine and glycine-rich protein 3 (Crp3) sensitizes SMCs to apoptosis induced by stretching. Building upon this finding, we aimed to investigate the influence of Crp3 on elastolysis and apoptosis during AAA development. Using the elastase-CaCl2 rat model, we observed an increase in Crp3 expression, aortic diameter, and a reduction in wall thickness in wild type rats. In contrast, Crp3−/− rats exhibited a decreased incidence of AAA, with minimal or no changes in aortic diameter and thickness. Histopathological analysis revealed the absence of SMC apoptosis and degradation of elastic fibers in Crp3−/− rats, accompanied by reduced inflammation and diminished proteolytic capacity in Crp3−/− SMCs and bone marrow-derived macrophages. Collectively, our findings provide evidence that Crp3 plays a crucial role in AAA development by modulating elastolysis, inflammation, and SMC apoptosis. These results underscore the potential significance of Crp3 in the context of AAA progression and offer new insights into therapeutic targets for this disease.
Family support during childhood as a predictor of mate retention and kin care in adults during the COVID-19 pandemic in Brazil: an exploratory study
Authors: Tasso Natália de Araújo Miranda , Castro Felipe Nalon
Published: December 2023

Introduction

Experiences during development help to explain behavior expression in adulthood.

Aims

In this study, we explored how unpredictability and harshness experienced during childhood may have impacted the occurrence of reproductive milestones in adulthood and the expression of fundamental motives related to self-protection, disease avoidance, mate seeking, mate retention, and kin care (children/family) during the pandemic.

Methods

This was an exploratory study with 438 participants. Through the administration of online questionnaires, participants were assessed and categorized based on their childhood experiences, resulting in three groups: low unpredictability, high unpredictability with family support, and high unpredictability without family support.

Results

We found that family support experienced during childhood predicts a slow life-history strategy. This involves an emphasis on growth and parenting efforts at the expense reproduction and was relevant even for participants who faced financial unpredictability. During the pandemic, we also observed that mate retention and kin care (family) motives were predominant among individuals who had greater family support during childhood.

Discussion

Overall, the findings suggest that unpredictability experienced during childhood is crucial for the development of life-history strategies and the manifestation of fundamental motives in adulthood.
Subliminal mortality salience does not increase physical strength output in double-blind randomized controlled trial
Authors: Bartenschlager Christopher T. J. , Jansen Petra
Published: December 2023

Introduction

Using the morality salience paradigm, this research tested whether subliminal death stimuli lead to increased physical strength. Moreover, it was investigated if mindfulness and self-esteem instability influence terror management.

Methods

In total, data from 160 undergraduate sports students were analyzed. Participants completed a word decision task in which they were presented with either the word death or pain for 28.5 ms. Before and after the task, their grip strength was measured using a hand dynamometer.

Results

Linear mixed models could neither confirm the effect of the mortality salience hypothesis on strength nor an influence of mindfulness and self-esteem.

Discussion

The results raise the question of a potential influence of subliminal mortality salience on athletic performance and how mindfulness and self-esteem instability affect terror management.
Contingent negative variation as an evaluation indicator of neurocognitive disorder after traumatic brain injury
Authors: Ling Xindi , Wang Shujian , Zhang Shengyu , Li Wen , Zhang Qinting , Cai Weixiong , Li Haozhe
Published: December 2023

Introduction

Neurocognitive disorders are commonly observed in patients suffering from traumatic brain injury (TBI). Methods to assess neurocognitive disorders have thus drawn the general attention of the public, especially electrophysiology parameter such as contingent negative variation (CNV), which has been given more emphasis as a neurophysiological marker in event-related potentials (ERPs) for diagnosing a neurocognitive disorder and assessing its severity. The present study focused on the correlations between CNV parameters and levels of daily living activities and social function to explore the potential of CNV as an objective assessment tool.

Methods

Thirty-one patients with a diagnosis of neurocognitive disorder after a TBI according to ICD-10 were enrolled as the patient group, and 24 matched healthy volunteers were enrolled as the control group. The activity of daily living scale, functional activities questionnaire, social disability screening schedule, and scale of personality change following TBI were used to assess daily living activity and social function.

Results

The scale scores in patients were significantly higher than those in controls. Maximum amplitudes before S2 and during the post-imperative negative variation (PINV) period were also significantly higher in the patient group compared to the control group and were positively correlated with four scale scores. The duration of PINV at Fz and Cz was significantly shorter in the patient group than in the control group. The CNV return to baseline from a positive wave at electrode Fz and Cz occurred significantly earlier in the control group than in the patient group, while at Pz, the result showed the opposite.

Conclusion

Lower amplitudes of CNV were associated with more severe neurocognitive disorder and greater impairments in daily life abilities and social function. The duration of PINV and the latency of returning to baseline from a positive wave were correlated with the neurocognitive disorder to some extent. CNV could be used as an objective, electrophysiology-based parameter for evaluating the severity of the neurocognitive disorder and personality changes after TBI.
Autistic discussion forums: insights into the topics that clinicians don’t know about
Authors: Caldwell-Harris Catherine L. , McGlowan Tiffany , Beitia Katherine
Published: December 2023

Background

User-led autism discussion forums provide a wealth of information about autistic lived experiences, albeit oriented toward those who regularly use computers. We contend that healthcare professionals should read autism discussion forums to gain insight, be informed, and in some cases, to correct assumptions about autistic persons’ lives and possibilities. But experts may be dismissive of user-led forums, believing forums to be filled with myths, misinformation, and combative postings. The questions motivating our research were: Do online forums raise issues that are educational for clinicians and other stakeholders? Are forums useful for those who do empirical research?

Method

Content analysis was conducted on 300 posts (62,000 words) from Reddit, Quora, and Wrong Planet. Forums were sampled to reflect broad topics; posts were selected sequentially from the identified forums. The authors read through posts in the Excel sheet, highlighting statements that were the main ideas of the post, to discern both broad categories of topics and more specific topics. We coded content pertinent to classic autism myths and analyzed attitudes towards myths such as ‘lack emotion’ and ‘cannot form relationships.’ To document whether forum posts discuss topics that are not widely known outside of elite experts, we compared discussion content to new material about autism contained in the March 2022 DSM 5 Text revision.

Results

Classic autism myths were discussed with examples of when elements of myths may be valid. Posters described cases where parents or therapists believed myths. Experts may believe autism myths due to rapid changes in diagnostic practices and due to their lack of knowledge regarding the characteristics of autistic people who have typical intellectual abilities. We conclude that forums contain high-value information for clinicians because all concepts in the DSM 5 text revision were discussed by posters in the years before the text revision appeared. Ideas that are only slowly becoming part of the research literature are discussed at length in forums. Reading and analyzing forums is useful for both clinicians and scientists. In addition, the relative ease of forum analysis lowers the bar for entry into the research process.
Compassion focused therapy for women in the perinatal period: a summary of the current literature
Authors: Millard Leah Alice , Wittkowski Anja
Published: December 2023

Introduction

Compassion focused therapy (CFT) is emerging as an effective psychological intervention to treat those experiencing mental health difficulties. CFT was adapted for women who are mothers during the perinatal period (from conception to 2 year postpartum). Although CFT is being delivered in NHS perinatal mental health services in the United Kingdom (UK), its current evidence-base for the treatment of women’s mental health problems is unclear. As part of this Mini Review, we aimed to identify the current findings relating to CFT for women in the perinatal period (with or without a mental health condition) in order to identify any associated future research and clinical implications.

Method

A systematic search of two databases was undertaken. Included studies were required to meet the following criteria: (1) offered an intervention using CFT or perinatal CFT (P-CFT), (2) participants were women in the perinatal period, and (3) studies used a pre- and post-intervention study design. No language restrictions were used. A narrative synthesis was then conducted.

Results

Five studies, dating from 2018 to 2023, met the inclusion criteria. A total of 1,258 participants were included across those studies. Significant improvements in compassion-based outcomes (i.e., self-compassion, self-criticism/self-reassurance) were observed. However, these findings were primarily derived from non-clinical samples (n = 4) and could only be seen as preliminary.

Conclusion

Although these results are encouraging for mothers presenting with sub-clinical mental health symptoms, further research is clearly warranted to determine whether CFT/P-CFT may benefit mothers, including those presenting with more significant perinatal mental health difficulties.
Integrating father involvement into early childhood initiatives delivered at scale: key considerations
Authors: Nair Sapna , Sinha Harshula , Holding Penny
Published: December 2023

Early child development (ECD) interventions, usually centered around the mother, exclude fathers from contributing to childcare. Research shows that a father’s involvement in childcare can have a positive impact on the child’s growth. In this light, a trial was conducted in rural India wherein a new program component aimed at enhancing fathers’ engagement in early child was incorporated into an existing childcare intervention. The paper highlights the learnings drawn from the trial to present pathways to change, that is, strategies needed to embed father involvement as a component within the intervention ecosystem.
Is education level, as a proxy for socio-economic position, related to device-measured and self-reported sedentary behavior in European older adults? A cross-sectional study from the SITLESS project
Authors: Rodriguez Roca Beatriz , Tully Mark A. , Sansano-Nadal Oriol , Caserotti Paolo , Coll-Planas Laura , Roqué Marta , Brønd Jan , Blackburn Nicole E. , Wilson Jason J. , Rothenbacher Dietrich , McIntosh Emma , Deidda Manuela , Andrade-Gómez Elena , Giné-Garriga Maria
Published: December 2023

Background

Sedentary behavior (SB) is a determinant of health in older adult people. Educational level is a primary driver of health disparities and is demonstrated to be a reliable measure of socioeconomic position. We aimed to examine the associations between educational level and self-reported along with device-measured SB in older adults living in Europe and the association of mentally active and passive SB domains with the educational level and gender in these associations.

Methods

The design is cross-sectional. One thousand three hundred and sixty participants aged 65 and over (75.3±6.3 years old, 61.8% women) participated. Inclusion criteria were scored with the Short Physical Performance Battery. Variables that describe the sample were assessed with an interview, and device-measured SB was assessed with an accelerometer. SB was assessed with the Sedentary Behavior Questionnaire and an accelerometer. Multiple linear regression models were used to study the association between the level of education and SB.

Results

Participants self-reported an average of 7.82 (SD: 3.02) daily waking hours of SB during weekend days, and the average of device-measured SB was 11.39 (1.23) h. Total mentally active SB (weekdays and weekends) was associated with the education level (p < 0.000). Participants were more sedentary during the week than during weekends, regardless of level of education (p < 0.000). Education level was significantly associated with self-reported mean hours per day in 46SB (p = 0.000; R=0.026; 95%CI).

Conclusion

Low education level in older adults is associated with self-reported SB but not with objective SB measures.
Girl–boy differences in perceptions of health determinants and cancer: a more systemic view of girls as young as 6 years
Authors: Gay Chloé , Deyra Maéliane , Berland Pauline , Gerbaud Laurent , Pizon Frank
Published: December 2023

Introduction

To model and analyze the differences between girls' and boys' conceptions of the determinants of health and cancer, as expressed and perceived by children and adolescents.

Method

A multicentric qualitative study was conducted in five schools (ages 6–11 years), four middle schools (ages 11–15 years), and three high schools (ages 15–18 years). A multi-phase protocol (phase 1 uses the e.Photoexpression© and phase 2 uses the Photonarration) enables children and teenagers to express themselves through photography and storytelling.

Results

A total of 4,174 qualitative productions were produced by 1,068 children, of which 47% were girls and 53% were boys, all in the ages of 6–18 years. From the results of the productions, it can be noticed that children mentioned and were aware of 30 determinants of health and cancer. The three determinants most mentioned were “Consumption of psychotropic drugs”, “Diet”, and “Harmful environment”. Among these 30 determinants, some were mentioned to a greater or lesser extent by girls and boys. These significant gender differences are present for 20 determinants of health and cancer. These differences evolve with age: (1) In elementary school (ages 6–11), girls gave significantly more importance (p < 0.05) to 11 determinants, while boys attached significantly more importance (p < 0.05) to 2 determinants. (2) In middle school (ages 11–16), girls gave significantly (p < 0.05) more importance to 12 determinants, while boys gave significantly (p < 0.05) more importance to one determinant. (3) In high school (ages 15–18 years), girls gave significantly (p < 0.05) more importance to 13 determinants. There was no significant difference (p < 0.05) in favor of boys for high school students. Girls also have a more systemic view of health determinants than boys. The increase in the number of determinants cited by girls is significant (p = 0.017) between the ages of 6–11 and 15–18 years. This gap widens with age (+1.45 determinants) for girls and (+0.68 determinants) for boys between elementary school and high school.

Conclusion

The determinants identified as predominantly female or male, as well as age-related specificities, constitute a resource for effective preventive action, as close as possible to the needs and particularities of a population. This mapping of people's conceptions could provide a decision-making aid in defining the strategic orientations of prevention policies.
Area-based social inequalities in adult mortality: construction of French deprivation-specific life tables for the period 2016–2018
Authors: Merville Ophélie , Rollet Quentin , Dejardin Olivier , Launay Ludivine , Guillaume Élodie , Launoy Guy
Published: December 2023

Background

In order to tackle social inequalities in mortality, it is crucial to quantify them. We produced French deprivation-specific life tables for the period 2016–2018 to measure the social gradient in adult all-cause mortality.

Methods

Data from the Permanent Demographic Sample (EDP) were used to provide population and death counts by age, sex and deprivation quintile. The European Deprivation Index (EDI), applied at a sub-municipal geographical level, was used as an ecological measure of deprivation. Smoothed mortality rates were calculated using a one-dimensional Poisson counts smoothing method with P-Splines. We calculated life expectancies by age, sex and deprivation quintile as well as interquartile mortality rate ratios (MRR).

Results

At the age of 30, the difference in life expectancy between the most and least deprived groups amounted to 3.9 years in males and 2.2 years in females. In terms of relative mortality inequalities, the largest gaps between extreme deprivation groups were around age 55 for males (MRR = 2.22 [2.0; 2.46] at age 55), around age 50 in females (MRR = 1.77 [1.48; 2.1] at age 47), and there was a decrease or disappearance of the gaps in the very older adults.

Conclusions

There is a strong social gradient in all-cause mortality in France for males and females. The methodology for building these deprivation-specific life tables is reproducible and could be used to monitor its development. The tables produced should contribute to improving studies on net survival inequalities for specific diseases by taking into account the pre-existing social gradient in all-cause mortality.
A metric learning-based method for biomedical entity linking
Authors: Le Ngoc D. , Nguyen Nhung T. H.
Published: December 2023

Biomedical entity linking task is the task of mapping mention(s) that occur in a particular textual context to a unique concept orentityin a knowledge base, e.g., the Unified Medical Language System (UMLS). One of the most challenging aspects of the entity linking task is the ambiguity of mentions, i.e., (1) mentions whose surface forms are very similar, but which map to different entities in different contexts, and (2) entities that can be expressed using diverse types of mentions. Recent studies have used BERT-based encoders to encode mentions and entities into distinguishable representations such that their similarity can be measured using distance metrics. However, most real-world biomedical datasets suffer from severe imbalance, i.e., some classes have many instances while others appear only once or are completely absent from the training data. A common way to address this issue is to down-sample the dataset, i.e., to reduce the number instances of the majority classes to make the dataset more balanced. In the context of entity linking, down-sampling reduces the ability of the model to comprehensively learn the representations of mentions in different contexts, which is very important. To tackle this issue, we propose a metric-based learning method that treats a given entity and its mentions as a whole, regardless of the number of mentions in the training set. Specifically, our method uses a triplet loss-based function in conjunction with a clustering technique to learn the representation of mentions and entities. Through evaluations on two challenging biomedical datasets, i.e., MedMentions and BC5CDR, we show that our proposed method is able to address the issue of imbalanced data and to perform competitively with other state-of-the-art models. Moreover, our method significantly reduces computational cost in both training and inference steps. Our source code is publicly availablehere.
System integration of magnetic medical microrobots: from design to control
Authors: Zhou Junjian , Li Mengyue , Li Na , Zhou Yuting , Wang Jingyi , Jiao Niandong
Published: December 2023

Magnetic microrobots are ideal for medical applications owing to their deep tissue penetration, precise control, and flexible movement. After decades of development, various magnetic microrobots have been used to achieve medical functions such as targeted delivery, cell manipulation, and minimally invasive surgery. This review introduces the research status and latest progress in the design and control systems of magnetic medical microrobots from a system integration perspective and summarizes the advantages and limitations of the research to provide a reference for developers. Finally, the future development direction of magnetic medical microrobot design and control systems are discussed.
Sleep in women: a narrative review of hormonal influences, sex differences and health implications
Authors: Andersen Monica L. , Hachul Helena , Ishikura Isabela Antunes , Tufik Sergio
Published: December 2023

Sleep is a fundamental biological behavior that affects various aspects of health and wellbeing. However, there are some differences in respect of sleep between men and women. Notably, there are sex differences in relation to sleep problems and the potential comorbidities, such as diabetes and cardiovascular diseases, that are associated with these problems, with some evidence suggesting that women may have a greater predisposition to sleep disturbances. This narrative review provides a comprehensive analysis of the literature in respect of sex differences in the sleep, with the main focus being on women. Basic research has investigated sex-specific distinctions in sleep architecture, sleep quality, and circadian rhythms, while clinical studies have examined sex differences in sleep disorders, such as insomnia, sleep apnea, and restless leg syndrome. This narrative review also highlights the impact of the periods of hormonal fluctuations that occur across a woman's lifespan - such as during the menstrual cycle, pregnancy, and menopause phase - and examines their effects on sleep. It also explores the influence of social and cultural factors on sleep patterns in women. Taken together, the evidence suggests that women may be more susceptible to sleep disturbance, and that gender-specific factors should be considered when evaluating sleep in clinical practice. Further research is warranted to elucidate the mechanisms that underlie this and help guide the development of sex-specific interventions to improve sleep quality and promote holistic health in women.
Public values failure associated with Hurricane Ian power outages
Authors: Entress Rebecca M. , Stevens Kelly A.
Published: December 2023

Power outages from extreme weather events can diminish community resilience, making it difficult for the areas impacted to bounce back after such events. For socially vulnerable populations, the frequency and duration of power outages can be even more severe. Governments have an obligation to protect public values, or those values that are most fundamental to society, which includes equitable resilience. Using Jørgensen and Bozeman's inventory of public values, this manuscript explores how power outages from extreme weather events create public values failures. More specifically, the manuscript evaluates intraorganizational aspects of public administration during power outages in Florida during Hurricane Ian in 2022. Framing power outages as a public values failure may motivate greater time and effort toward improving equitable access to more resilient power systems.
A nomogram model to predict the portal vein thrombosis risk after surgery in patients with pancreatic cancer
Authors: Wang Jing , Wang Hanxuan , Li Binglin , Cui Songping , Lyu Shaocheng , Lang Ren
Published: December 2023

Background

Portal vein thrombosis (PVT) is a common postoperative complication in patients with pancreatic cancer (PC), significantly affecting their quality of life and long-term prognosis. Our aim is to establish a new nomogram to predict the risk of PVT after PC surgery.

Method

We collected data from 416 patients who underwent PC surgery at our hospital between January 2011 and June 2022. This includes 87 patients with PVT and 329 patients without PVT. The patients were randomly divided into a training group and a validation group at a ratio of 7:3. We constructed a nomogram model using the outcomes from both univariate and multivariate logistic regression analyses conducted on the training group. The nomogram’s predictive capacity was assessed using calibration curve, receiver operating characteristic (ROC) curve, and decision curve analysis (DCA).

Results

In the study, the prevalence of PVT was 20.9%. Age, albumin, vein reconstruction and preoperative D-dimer were independent related factors. The model achieved a C-index of 0.810 (95% confidence interval: 0.752–0.867), demonstrating excellent discrimination and calibration performance. The area under the ROC curve of the nomogram was 0.829 (95% CI: 0.750–0.909) in the validation group. DCA confirmed that the nomogram model was clinically useful when the incidence of PVT in patients was 5%–60%.

Conclusion

We have established a high-performance nomogram for predicting the risk of PVT in patients undergoing PC surgery. This will assist clinical doctors in identifying individuals at high risk of PVT and taking appropriate preventive measures.
Using Markov Random Field and Analytic Hierarchy Process to Account for Interdependent Criteria
Authors: Huang Jih-Jeng , Chen Chin-Yi
Published: December 2023

The Analytic Hierarchy Process (AHP) has been a widely used multi-criteria decision-making (MCDM) method since the 1980s because of its simplicity and rationality. However, the conventional AHP assumes criteria independence, which is not always accurate in realistic scenarios where interdependencies between criteria exist. Several methods have been proposed to relax the postulation of the independent criteria in the AHP, e.g., the Analytic Network Process (ANP). However, these methods usually need a number of pairwise comparison matrices (PCMs) and make it hard to apply to a complicated and large-scale problem. This paper presents a groundbreaking approach to address this issue by incorporating discrete Markov Random Fields (MRFs) into the AHP framework. Our method enhances decision making by effectively and sensibly capturing interdependencies among criteria, reflecting actual weights. Moreover, we showcase a numerical example to illustrate the proposed method and compare the results with the conventional AHP and Fuzzy Cognitive Map (FCM). The findings highlight our method’s ability to influence global priority values and the ranking of alternatives when considering interdependencies between criteria. These results suggest that the introduced method provides a flexible and adaptable framework for modeling interdependencies between criteria, ultimately leading to more accurate and reliable decision-making outcomes.
Identifying Constituent Elements of Entrepreneurship Curricula: A Systematic Literature Review
Authors: Tiberius Victor , Weyland Michael
Published: December 2023

Entrepreneurship education research has a strong “output” focus on impact studies but pays much less attention to the “inside” or process perspective of the way entrepreneurship education occurs. In particular, the scattered previous entrepreneurship curriculum research has not managed to provide a current and comprehensive overview of the curricular elements that constitute entrepreneurship education. To overcome this shortcoming, we aim to identify the teaching objectives, teaching contents, teaching methods, and assessment methods discussed in entrepreneurship curriculum research. To this end, we conducted a systematic literature review on the four entrepreneurship curriculum dimensions and collected all mentioned curriculum items. We used a two-stage coding procedure to find the genuinely entrepreneurship-specific items. Among numerous items (also from business management and other subjects), we found 26 objectives, 34 contents, 11 teaching methods, and 7 assessment methods that were entrepreneurship-specific. Most of these items were addressed by only a few scholarly papers.
Three-Dimensional Trajectory and Impingement Simulation of Ice Crystals Considering State Changes on the Rotor Blade of an Axial Fan
Authors: Hirose Koichiro , Fukudome Koji , Mamori Hiroya , Yamamoto Makoto
Published: December 2023

Ice crystal icing occurs in jet engine compressors, which can severely degrade jet engine performance. In this study, we developed an ice crystal trajectory simulation, considering the state changes of ice crystals with a forced convection model, indicating a significant difference in impinging ice crystal content on the blade for tiny ice crystals. Then, ice crystal trajectory simulations were performed for the rotor blade of an axial fan to investigate the effects of ice crystal size and relative humidity on collision characteristics. The results indicate that the surrounding air affects the composition of tiny ice crystals before collision, and the flight time until impingement on the rotor blade varies significantly depending on the span position. Among them, ice crystals with a diameter of 50 μm impinge with water content that is most likely to adhere to the blade. Three-dimensional simulation results show that many ice crystals impinge not only on the leading edge, where icing occurs as revealed by the two-dimensional simulations but also on the trailing edge of the hub side. This study emphasizes the importance of evaluating the three-dimensional impingement position and water content in the prediction of ice crystal icing.
Investigation of ABO Gene Variants across More Than 60 Pig Breeds and Populations and Other Suidae Species Using Whole-Genome Sequencing Datasets
Authors: Bolner Matteo , Bertolini Francesca , Bovo Samuele , Schiavo Giuseppina , Fontanesi Luca
Published: December 2023

Polymorphisms in the human ABO gene determine the major blood classification system based on the three well-known forms: A; B; and O. In pigs that carry only two main alleles in this gene (A and O), we still need to obtain a more comprehensive distribution of variants, which could also impact its function. In this study, we mined more than 500 whole-genome sequencing datasets to obtain information on the ABO gene in different Suidae species, pig breeds, and populations and provide (i) a comprehensive distribution of the A and O alleles, (ii) evolutionary relationships of ABO gene sequences across Suidae species, and (iii) an exploratory evaluation of the effect of the different ABO gene variants on production traits and blood-related parameters in Italian Large White pigs. We confirmed that allele O is likely under balancing selection, present in all Sus species investigated, without being fixed in any of them. We reported a novel structural variant in perfect linkage disequilibrium with allele O that made it possible to estimate the evolutionary time window of occurrence of this functional allele. We also identified two single nucleotide polymorphisms that were suggestively associated with plasma magnesium levels in pigs. Other studies can also be constructed over our results to further evaluate the effect of this gene on economically relevant traits and basic biological functions.
Molecular Detection and Genetic Characterization of Vertically Transmitted Viruses in Ducks
Authors: Wang Xinrong , Yu Haidong , Zhang Wenli , Fu Lizhi , Wang Yue
Published: December 2023

To investigate the distribution and genetic variation in four vertically transmitted duck pathogens, including duck hepatitis B virus (DHBV), duck circovirus (DuCV), duck hepatitis A virus 3 (DHAV-3), and avian reoviruses (ARV), we conducted an epidemiology study using PCR and RT-PCR assays on a duck population. We found that DHBV was the most prevalent virus (69.74%), followed by DuCV (39.48%), and then ARV (19.92%) and DHAV-3 (8.49%). Among the 271 duck samples, two, three or four viruses were detected in the same samples, indicating that the coinfection of vertical transmission agents is common in ducks. The genetic analysis results showed that all four identified DuCV strains belonged to genotype 1, the DHAV-3 strain was closely clustered with previously identified strains from China, and the ARV stain was clustered under genotype 1. These indicate that different viral strains are circulating among the ducks. Our findings will improve the knowledge of the evolution of DuCV, DHAV-3, and ARV, and help choose suitable strains for vaccination.
Proteomic Analysis Identifies Distinct Protein Patterns for High Ovulation in FecB Mutant Small Tail Han Sheep Granulosa Cells
Authors: Wang Xiangyu , Guo Xiaofei , He Xiaoyun , Di Ran , Zhang Xiaosheng , Zhang Jinlong , Chu Mingxing
Published: December 2023

The Booroola fecundity (FecB) mutation in the bone morphogenetic protein receptor type 1B (BMPR1B) gene increases ovulation in sheep. However, its effect on follicular maturation is not fully understood. Therefore, we collected granulosa cells (GCs) at a critical stage of follicle maturation from nine wild-type (WW), nine heterozygous FecB mutant (WB), and nine homozygous FecB mutant (BB) Small Tail Han sheep. The GCs of three ewes were selected at random from each genotype and consolidated into a single group, yielding a total of nine groups (three groups per genotype) for proteomic analysis. The tandem mass tag technique was utilized to ascertain the specific proteins linked to multiple ovulation in the various FecB genotypes. Using a general linear model, we identified 199 proteins significantly affected by the FecB mutation with the LIMMA package (p < 0.05). The differential abundance of proteins was enriched in pathways related to cholesterol metabolism, carbohydrate metabolism, amino acid biosynthesis, and glutathione metabolism. These pathways are involved in important processes for GC-regulated ‘conservation’ of oocyte maturation. Further, the sparse partial least-squares discriminant analysis and the Fuzzy-C-mean clustering method were combined to estimate weights and cluster differential abundance proteins according to ovulation to screen important ovulation-related proteins. Among them, ZP2 and ZP3 were found to be enriched in the cellular component catalog term “egg coat”, as well as some apolipoproteins, such as APOA1, APOA2, and APOA4, enriched in several Gene Ontology terms related to cholesterol metabolism and lipoprotein transport. A higher abundance of these essential proteins for oocyte maturation was observed in BB and WB genotypes compared with WW ewes. These proteins had a high weight in the model for discriminating sheep with different FecB genotypes. These findings provide new insight that the FecB mutant in GCs improves nutrient metabolism, leading to better oocyte maturation by altering the abundance of important proteins (ZP2, ZP3, and APOA1) in favor of increased ovulation or better oocyte quality.
Spermatological Characterization of the Cestode Meggittina gerbilli (Cyclophyllidea: Catenotaeniidae), a Parasite of Gerbils, Gerbillus gerbillus and Gerbillus campestris (Rodentia: Muridae) in Tunisia
Authors: Aouina Faouzi , Kacem Hichem , Martín-Carrillo Natalia , Foronda Pilar , Miquel Jordi
Published: December 2023

Ultrastructural characters of spermiogenesis and the mature spermatozoon of the cestode Meggittina gerbilli (Cyclophyllidea: Catenotaeniidae), a parasite of the Lesser Egyptian gerbil (Gerbillus gerbillus) and the North African gerbil (Gerbillus campestris) (Rodentia: Muridae) in the Djebel Dahar (South of Tunisia), were studied using transmission electron microscopy. The spermiogenesis of M. gerbilli is of Bâ and Marchand’s type III, which is mainly characterized by a proximodistal fusion of a single flagellum with a cytoplasmic extension. In this catenotaeniid, the proximal fusion is preceded by a 90° rotation of the flagellum. The spermatozoon is a Levron et al. type VI, which presents a single axoneme with the 9 + ‘1’ trepaxonematan pattern, a periaxonemal sheath, two crest-like bodies, twisted cortical microtubules, and a spiraled nucleus. The obtained results show similarities with the remaining studied catenotaeniids, namely Catenotaenia pusilla and Skrjabinotaenia lobata. The results are compared and discussed according to several characteristics found in the catenotaeniids and other studied cyclophyllideans.
African Swine Fever and Its Control Measures in Wild Boar: A “De Iure Condito” Analysis in the European Union
Authors: Migliore Sergio , Hussein Hany A. , Galluzzo Paola , Puleio Roberto , Loria Guido Ruggero
Published: December 2023

Recently, the African swine fever (ASF) epizootic has been reported in domestic pigs and wild boars in several European Union Member States (EU MS) and epidemiological evidence has accumulated which indicates that wild boar play a key role in maintaining and spreading the disease. Thanks to the experience gained when managing ASF outbreaks in Sardinia (Italy) and Eastern Europe, Directive 2002/60 CE was issued. This directive represented an important step forward in controlling the disease, particularly the risk of spreading the virus to wild animals. Since 2021, according to Regulation (EU) 2016/429, which is also called “Animal Health Law—AHL”, when the MS competent authority suspects or confirms ASF (a cat. A listed disease) in wild animals, it is mandatory to conduct surveillance in the wild boar population and implement the necessary control measures. Within AHL, Regulations (EU) 2020/687 and 2023/594 established special ASF control measures in kept and wild porcine animals, and their products and by-products, focusing on and underlying old and new responsibilities that vets (both public and private ones) have to accomplish under the new regulations. The new change in the legal framework deals with specific measures to be applied in the wild and represents a great challenge for MS veterinary services. Some of these measures have been well established in the last two decades, particularly those related to application in the farming system, while other measures are still new to veterinary health management and require a holistic approach in terms of intensity, considering all geographical, ecological, productive, cultural and social features of the involved EU MS. In this contribution, the authors intend to focus on specific measures which have been issued in order to limit or stop the spread of ASF in a wild, “boundless” ecosystem. These measures expand the field of competence of the official veterinary service to wild areas in addition to farm activity.
Dysregulation of Ceramide Metabolism Is Linked to Iron Deposition and Activation of Related Pathways in the Aorta of Atherosclerotic Miniature Pigs
Authors: Cai Zhaowei , Deng Liqun , Fan Yingying , Ren Yujie , Ling Yun , Tu Jue , Cai Yueqin , Xu Xiaoping , Chen Minli
Published: December 2023

The miniature pig is a suitable animal model for investigating human cardiovascular diseases. Nevertheless, the alterations in lipid metabolism within atherosclerotic plaques of miniature pigs, along with the underlying mechanisms, remain to be comprehensively elucidated. In this study, we aim to examine the alterations in lipid composition and associated pathways in the abdominal aorta of atherosclerotic pigs induced by a high-fat, high-cholesterol, and high-fructose (HFCF) diet using lipidomics and RNA-Seq methods. The results showed that the content and composition of aortic lipid species, particularly ceramide, hexosyl ceramide, lysophosphatidylcholine, and triglyceride, were significantly altered in HFCF-fed pigs. Meanwhile, the genes governing sphingolipid metabolism, iron ion homeostasis, apoptosis, and the inflammatory response were significantly regulated by the HFCF diet. Furthermore, C16 ceramide could promote iron deposition in RAW264.7 cells, leading to increased intracellular reactive oxygen species (ROS) production, apoptosis, and activation of the toll-like receptor 4 (TLR4)/nuclear Factor-kappa B (NF-қB) inflammatory pathway, which could be mitigated by deferoxamine. Our study demonstrated that dysregulated ceramide metabolism could increase ROS production, apoptosis, and inflammatory pathway activation in macrophages by inducing iron overload, thus playing a vital role in the pathogenesis of atherosclerosis. This discovery could potentially provide a new target for pharmacological therapy of cardiovascular diseases such as atherosclerosis.