bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGGALIAN DAN INVENTARISASI 1000 JUDUL NASKAH MELAYU DI BANDA ACEH
Authors: AR Nurdin
Published: April 2018

The most Indonesian written manuscripts were written in the Netherlands language. These manuscripts could be accessed by one of the following catalogs: Juynboll Catalogue (1899), Ronkel Catalogues (1909), Riclefs and P. Voorhoeve Catalogue (1977) etc. Unfortunately, the Indonesian researchers have no way to utilize them. According to the international catalogs written in Indonesian and foreign languages, The Malay manuscripts found in Banda Aceh reached about 300 titles. After conducting preliminary research and direct observation to the individual and institutional collectors, it was found that the real numbers of them could reach to a thousand titles of manuscripts that still have separated in various local places. On behalf these facts, the research aims to rescue these manuscripts from a disappearing and extinction by conducting inventory that is predicted to be able to push the Indonesian researchers ahead to discovering their substantial contents that in opinion useful for human life. The output of the research is compiling catalog documents of 1000 titles of Malay manuscripts found in Banda Aceh.
The theory and method used in this research are the theory of the codicology and the method of the catalog of manuscripts register. The codicology is subfield studying all of the manuscript’s aspects in details such as the place of storing, the owner of the manuscript, the content of the manuscript, etc. Whereas the catalog of a manuscript register is describing all of systematically manuscript’s physical and objective aspect in details. The results of the research will be proposed to be the main gate for entering later philology works i.e. presenting and interpreting the text that in turn can be positioned as a manual directive in social and national life.
Sweet Onion (Allium cepa L.) as Influenced by Organic Fertilization Rate: 2. Bulb Yield and Quality before and after Storage
Authors: Díaz-Pérez Juan Carlos , Bautista Jesús , Gunawan Gunawati , Bateman Anthony , Riner Cliff Martin
Published: April 2018

There is a growing interest in organic fertilizers because of increased demand for organic sweet onions and other vegetables. There are, however, limited studies on sweet onion bulb yield and quality in response to organic fertilization. The objective of this study was to evaluate the effects of organic fertilizer rate on sweet onion bulb yield and bulb quality before and after storage. Experiments were conducted at the Horticulture Farm, Tifton Campus, University of Georgia, in the Winters of 2012–13 and 2013–14. There were five organic fertilization treatments (organic fertilizer 3–2–3 equivalent to 0, 60, 120, 180, and 240 kg·ha−1 N). Total and marketable yields and individual bulb weight increased quadratically with increasing organic fertilization rate and responses failed to reach a plateau. The fraction of extra-large bulb increased with increasing organic fertilization rate. Incidence of onion bolting was maximal at 60 kg·ha−1 N and decreased with increasing organic fertilization rate. The percentage of bulb dry weight was highest in the unfertilized control and decreased with increasing organic fertilization rate. Organic fertilization rate had no consistent impact on bulb soluble solids content (SCC) and pungency (measured as pyruvate concentration) in the two seasons. Total antioxidant capacity (measured as gallic acid equivalents) values were among the lowest at 60 and 120 kg·ha−1 N. In conclusion, onion bulb yields increased with increasing organic fertilization rate, whereas incidences of bulb diseases responded differently to N rate. Botrytis rot was the main cause of postharvest bulb decay in all organic fertilization rates.
Relay-cropping and Fallow Programs for Strawberry-based Production System: Effects on Crop Productivity and Weed Control
Authors: Yu Jialin , Boyd Nathan S. , Guan Zhengfei
Published: April 2018

Many strawberry growers in Florida relay crop vegetables with strawberries or grow multiple crops on the same plastic mulch. The practice can reduce the overall input costs per crop but weed management can be problematic. Field experiments designed as a split plot were conducted in Balm and Dover, FL over two successive strawberry-growing seasons from Oct. 2014 to Mar. 2015 (year 1) and Oct. 2015 to Mar. 2016 (year 2) and two successive muskmelon-growing seasons from March to July 2015 (year 1) and March to July 2016 (year 2). The objectives were to examine the effect of summer fallow programs and the presence or absence of a relay-crop on weed density and strawberry (Fragaria ×ananassa Duchesne) and muskmelon (Cucumis melo L.) yields. Summer fallow programs included leaving the plastic mulch in place and reusing it in year 2, a sunn hemp (Crotalaria juncea L.) cover crop, or a conventional chemical fallow. Relay cropping muskmelon with strawberries had no effect on strawberry yield. Summer fallow programs had no effect on muskmelon growth and yield in Balm and Dover, as well as strawberry growth and yield in Balm. In Dover, the plastic mulch summer fallow had 22% to 34% lower berry yield in year 2 compared with cover crop and chemical fallow, respectively. In year 2, relay-cropping was more effective in reducing total weed density compared with strawberry monoculture in Dover but not in Balm. In year 2 in Dover, averaged overall summer fallow programs, the total weed density was ≈3-fold less in relay-cropping than strawberry monoculture. Of all the summer fallow programs evaluated, leaving the plastic mulch in place combined with glyphosate was the most effective summer fallow program, whereas the conventional chemical fallow was the least effective at weed suppression. We conclude that relay cropping or double use of plastic mulch for successive strawberry crops are viable options for Florida strawberry growers.
Cross Breeding New Cultivars of Early-flowering Multiflora Chrysanthemum Based on Mathematical Analysis
Authors: Zhang Mengmeng , Huang He , Wang Qing , Dai Silan
Published: April 2018

Conventional crossbreeding remains an effective technique for chrysanthemum (Chrysanthemum ×morifolium Ramat.) breeding. However, there are always many problems when breeding chrysanthemum because of its complex genetic background, such as difficulty matching parents, selecting superior hybrid progenies, quantitatively describing certain target traits, and evaluating breeding results. A recent mathematical analysis method is an effective method for evaluating plant breeding progress. In this study, we used 505 multiflora chrysanthemum germplasm resources as test materials; we divided the flowering time into five groups using a grading analysis method, including extremely early group (genotypes that flowered when daylength was longer than 13.5 hours), early group (genotypes that flowered when daylength was 13.5–12.0 hours), medium group (genotypes that flowered when daylength was 12.0–11.0 hours), late group (genotypes that flowered when daylength was 11.0–10.0 hours), and extremely late group (genotypes that flowered when daylength was shorter than 10.0 hours). Moreover, the breeding objective was to breed early-flowering genotypes. Using 15 phenotypic characters as evaluation factors, 37 excellent genotypes, including four early-flowering genotypes, were screened out from the aforementioned resources according to an analytic hierarchy process (AHP) and weighting of the gray relational grade. We selected one early-flowering genotype and eight medium-flowering genotypes from these 37 genotypes and matched six hybridized combinations based on the genetic distance between genotypes calculated by the Q cluster analysis method. We used a comprehensive evaluation method combining AHP and the gray relational analysis (GRA) method for the evaluation of 367 progenies. Moreover, we screened out 52 superior hybrids, including 36 early-flowering hybrids. The results of this study demonstrate that the mathematical analysis method is an immensely effective method to breed new cultivars of early-flowering multiflora chrysanthemum. This study also provides an effective method to define and improve the flowering time of other cultivated plants.
In Vitro Seed Germination of Kalmia latifolia L. Hybrids: A Means for Improving Germination and Speeding Up Breeding Cycle
Authors: Li He , Zhang Donglin
Published: April 2018

Kalmia latifolia L. (mountain laurel), a member of Ericaceae, is a beautiful ornamental shrub native to the eastern United States. The plant is not common in the southeastern United States landscapes because of the limited heat tolerance of most commercial cultivars. Breeding of heat-tolerant cultivars can be achieved by cross hybridization, but is often challenged by low germination percentage, long germination time, and potential abortion of cross-hybridized seeds. We used in vitro seed germination to enhance germination and shorten germination time and investigated the appropriate collecting time, optimal basal medium, and pH for this approach. Collecting time affected in vitro seed germination, with more mature hybrid seeds [collected 4–5 months after pollination (MAP)] having higher germination rate (90% in 4 weeks) than the less mature seeds collected in 2 MAP (20% in 7 weeks). Seedlings from the mature seeds also produced two true leaves on average after 8 weeks of culture, whereas seedlings from the less mature seeds had no true leaves. Woody Plant Medium (WPM) better enhanced in vitro seed germination compared with Murashige and Skoog (MS) or Gamborg’s B5 (B5) medium. WPM yielded higher germination (98%) than MS (90%) and significantly greater total leaf area per seedling (67 mm3) than MS (50 mm3) and B5 (52 mm3) for seeds of ‘Firecracker’ × ‘Snowdrift’. Similar effects had been observed on seeds from ‘Little Linda’ × ‘Starburst’ and ‘Pristine’ × ‘Peppermint’. The pH ranging from 4.2 to 5.4 did not affect seed germination and seedling development of mountain laurel hybrids. Our protocol enabled early collection of mountain laurel hybrid seeds 1 month before their full maturation and permitted seeds to germinate in 4 weeks on WPM, which shortened the period from crossing to the seedling stage from up to 15 to 6 months and enhanced germination percentage from 30% to more than 90% compared with traditional seed germination. This protocol should be applied to promote the breeding and selection of new mountain laurel cultivars for the southeastern United States landscapes.
A PROTEÇÃO DOS DIREITOS HUMANOS LGBT E OS PRINCÍPIOS CONSAGRADOS CONTRA A DISCRIMINAÇÃO ATENTATÓRIA
Authors: Siqueira Dirceu Pereira , Machado Robson Aparecido
Published: April 2018

Este artigo tem por objetivo refletir sobre a importância e necessidade de uma proteção estatal para as pessoas LGBT, já que pertencem a uma minoria social e sexual e têm seus direitos humanos reconhecidos pela ONU, bem como a Constituição Federal prevê o princípio da igualdade no caput do artigo 5º e, ainda, dispõe que “a lei punirá qualquer discriminação atentatória dos direitos e liberdades fundamentais”, no inciso XLI, do artigo 5º. Uma das formas de garantia dessa igualdade são as políticas públicas para o combate das discriminações homofóbicas possibilitando a participação destas minorias no processo político e acesso à educação e emprego.
ENTRE A JUSTICIALIZAÇÃO PRIVADA E O ESTADO DE EXCEÇÃO: A (RE)CRIAÇÃO ANÔMICA DA SOCIEDADE
Authors: Copetti Neto Alfredo , Oliveira Ramires Linckse Bianca
Published: April 2018

O estudo aqui apresentado faz uma análise sobre a violência na atual conjuntura, mormente no que se refere à justiça privada – linchamentos – como meio de resolução imediata de conflitos, bem como a forma como o Estado, através de seu Soberano, se impõe diante da atual crise social. Traz à tona a questão sobre a tensão existente entre a obediência e a transgressão da norma estabelecida e, para tanto, parte do exemplo privilegiado de Antígona para abordar o conflito entre o direito positivo e direito natural.  Segue expondo os casos e as explicações acerca do fenômeno social da justicialização privada e, na sequencia, exibe a forma com que o Estado também tem feito justiça com as próprias mãos, através deste novo paradigma de governo que se instala sob a forma de exceção. Para melhor compreensão do tema é feita uma análise utilizando-se das obras de Giorgio Agamben e Gilberto Bercovici, as quais sugerem a possibilidade de um estado de exceção permanente diante das condutas arbitrárias de governo.
INTEGRATED APPROACH TO IMPROVING THE EFFICIENCY OF THE USE OF HUMAN CAPITAL IN THE REGION
Authors: Халиулина Валентина , Khaliulina Valentina , Шабашев В. , Shabashev V. , Шабашева С. , Shabasheva S.
Published: April 2018

The article deals with the constituent elements of the human capital and their influence on the development of the region. It stresses the special role of health as an important component of human capital in increasing production efficiency. The paper describes the current approaches to the definition of labor and human resources at the individual and community level (enterprise, region, society). It features the directions of their rational use in the case of transport enterprises of Kemerovo-city and the Kemerovo region. The authors give specific proposals for its improvement. The study involves a sociological survey revealing the motivation of the personnel as
well as facts of violations in the sphere of labor relations, and their interconnection. Attention is focused on ways to counteract the management of enterprises in case of violation of labor rights. The authors emphasize the need for social responsibility in the
maintenance and development of human capital, especially the need for an integrated approach to the effective use of human capital in the region.
DIVERSIFICATION OF SINGLE-INDUSTRY TOWNS' ECONOMY: THE ROLE OF NETWORKING
Authors: Шерешева М. , Sheresheva M.
Published: April 2018

The paper focuses on the diversification of single-industry towns’ economy and identifies the role of networking in this process. The author presents preliminary results of the Russian Science Foundation project "Sustainable development of the territorial
economy through networking of small cities and district centers". The goal of the project is to identify opportunities to overcome the growing gap in the level and quality of life in small Russian towns vs. large cities and megacities, and to propose measures for combining
resources and competencies of small cities in the framework of network relationships. The cases of Tula, Vladimir and Perm regions were in the focus of research. To achieve the research goal, a set of methods was applicable, including system analysis, formal logical
method, dialectical method, as well as quantitative and qualitative marketing research. This allows us to draw conclusions about the presence of structural problems and contemporary trends, and to offer recommendations taking into account the specialization of small singleindustry towns. Serious problems faced by the municipalities of single-industry towns, and the obstacles to efficient inter-municipal cooperation have been identified. At the same time, international evidence shows that the formation of a favorable institutional
environment for mutually beneficial combination of resources and competences of singleindustry towns with different specialization facilitates the implementation of joint projects and makes it possible to accelerate the processes of economic diversification by using the
benefits of a network coordination mechanism
TRANSFORMATION OF AGRICULTURAL EDUCATION SYSTEM AS A FACTOR OF SUSTAINABLE DEVELOPMENT OF THE AGRARIAN AND INDUSTRIAL COMPLEX IN THE REPUBLIC OF CRIMEA
Authors: Анисимова Н. , Anisimova N.
Published: April 2018

The paper features theoretical and methodical approaches to the
classification of regions from positions of modern region studies. It contains social and economic characteristics of the Republic of Crimea as an agrarian region. The author has monitored the current state of agrarian and industrial complex of the Republic of Crimea. The research has revealed that although agriculture is responsible for 17,1 % of the gross regional product, only 5,0 % of labor capacity of the region are engaged in the agrarian sphere of economy, and only 6,3 % of Crimean students major in agriculture. A complex analysis of the Crimean agrarian complex has revealed that the problem of qualified personnel deficiency is not acute for the regional economy, as there is an agricultural institute, as well as a number of minor vocational education institutions. A more relevant task is to improve the quality of training of young specialists and to attract them into rural areas; for this purpose a system and target
approach to agrarian education has been established in the region. The transformation of agricultural education in the republic should be based on the model of a wellbalanced interindustrial staffing plan of agrarian and industrial complex according to sustainable development programs of rural territories in the re gion.
THE QUALITY OF EMPLOYMENT OF GRADUATES ON REGIONAL LABOUR MARKETS
Authors: Стукен Т. , Stuken T.
Published: April 2018

The current research features employment of university graduates in the Siberian Federal District. The objective is to assess the quality of employment among higher education system graduates. The study is based on microdata obtained from a survey on employment conducted by the Federal Service of State Statistics (Rosstat). The methodology is based on a comparison of employment haracteristics (the level and profile of education, formal / informal, full / part-time employment) that reflect a possible demand for graduates on the local labour market. The main research methods are descriptive and regression analysis. The obtained results indicate that approximately one third of graduates fail to find work according to their specialty and perform jobs that do not require higher education. Every eighth graduate is employed in the informal sector. These figures indicate a lower quality of employment of graduates in the Siberian Federal District, compared with the average indicators for Russia. A regression analysis has showed that employment in accordance with the level and profile of education is determined mainly by individual characteristics of individuals. The best chances are in the field of medicine, the worst – in agriculture. The assumptions about
a high demand for specialists in the field of engineering and technology and a lower demand for specialists in economics and management have not been confirmed. These employment rates turned out to be approximately equal and correspond to average values. The obtained results can be used in the formation of programs of education development (primarily at the level of flagship universities) and improvement of educational programs.
PRESERVATION AND DEVELOPMENT OF MONO-TOWNS AND MONO-SETTLEMENTS
Authors: Демьяненко Л. , Demyanenko L.
Published: April 2018

Russia's integration into the world economy has had a negative impact on the socio-economic situation of single-industry towns and monopolies. The state allocates billions of dollars to support such monostructures, but the standard of living of the population is consistently deteriorating. The main reason for this situation is the
current mechanisms of state support that are focused exclusively on the creation of innovative industries. Legal acts regulating innovation support are formed in a hurry, without taking into account the specifics of the regional economy; moreover, they lack clear recommendations for the implementation of innovative projects. Grants are not provided in time, which prevents regions from implementing projects and planning their own budgets. The mechanism of providing budget funds via the development Fund of
single-industry towns, which is not a member of the budget process, places the process of spending budget funds outside the legal field, which excludes proper control over the expenditure of budget funds. In this situation the city-forming enterprises that define the main profile of education are deprived of any federal support
SELECTION OF PRIORITIES FOR SCIENTIFIC RESEARCH AND DEVELOPMENT AT A HIGHER EDUCATION INSTITUTION
Authors: Бянкин А. , Byankin A. , Бурдакова Г. , Burdakova G.
Published: April 2018

A rational choice of priorities in the field of research and development at the university is a fundamental factor in obtaining support, funding and commercialization. This choice depends on the technical, economic, environmental, educational and humanitarian problems of regions and cities where the higher education institution is located. The choice of priorities in the field of scientific research and development depends on a number of factors, such as continuous research; research interests of the academic staff; the curriculum; access to good laboratory facilities; the relevance for the Russian scientific community; the relevance for the world scientific community; the interests of scientific knowledge; demand from the customer (organization, enterprise); "The National Technology Initiative" state program; the priority development directions of science and technology; list of critical technologies of the Russian Federation; the Foresight method; funds, grants, and subsidies. The aim of the study is to analyze the process of selection of priorities for scientific-research work conducted by employees of the Komsomolskon-Amur state University (KnaGU). The authors considered the dynamics of scientific research and development in Russia and at the university. Based on a survey of university staff, the authors have identified approaches to the choice of research topics. The paper features some recommendations for selecting research priorities
Effects of farmland vegetation row direction on overland flow hydraulic characteristics
Authors: Zhang Shengtang , Zhang Jingzhou , Liu Yin , Liu Yuanchen
Published: April 2018

Soil erosion of farmland is a major issue faced by the agricultural industry. Control of water-induced erosion can be improved through the simulation of different configurations of vegetation row direction to analyze the effects of farmland vegetation row directions RD on the hydraulic characteristics of flow. The farmland vegetation row directions were simulated and the anisotropic influence of the farmland vegetation row direction on flow hydraulic characteristics was experimentally analyzed. Three groups of farmland vegetation row directions with angles θ of 15°, 45°, and 90° with respect to the flow direction FD were configured. Experimental results show that different angles caused variation in hydraulic parameters velocity V, Reynolds number Re, Froude number Fr, and Darcy–Weisbach friction factor λ along the directions of flow. The rates of change of λ, Re, and Fr were analyzed statistically, where θ was increased every 10°; the average rate of decrease of λ was 5.2% and the average rates of increase of Re and Fr were 2.2% and 3.4%, respectively. These results indicate that the vegetation distribution pattern on farmland has an anisotropic influence on the hydraulic characteristics of flow. This investigation provides valuable references for the optimization of farmland vegetation distribution and soil erosion control.
Exploring geometrical patterns in streamflow time series: utility for forecasting?
Authors: Teegavarapu Ramesh S. V.
Published: April 2018

Streamflow time series often provide valuable insights into the underlying physical processes that govern responses of any watershed to storm events. Patterns derived from time series based on repeated structures within these series can be beneficial for developing new or improved data-driven forecasting models. Data-driven models, artificial neural networks (ANN), are developed in the current study for streamflow prediction using input structures that are classified by geometrically similar patterns. A new modular and integrated ANN architecture that combines multiple ANN models, referred to as pattern-classified neural network (PCNN), is proposed, developed and investigated in this study. The PCNN relies on the development of several independent local models instead of one global data-driven prediction model. The PCNN models are evaluated for one step-ahead prediction of daily streamflows for Reed Creek and Little River, Virginia, and Elkhorn Creek, Kentucky in the United States. Results obtained from this study suggest that the use of these patterns has improved the performance of the neural networks in prediction. The improved performance of the PCNN models can be attributed to prior classification of data benefiting generalization abilities. PCNN model outputs can also provide an ensemble of forecasts that help quantify forecast uncertainty.
RURAL TOURISM IN INDIA CHALLENGES AND OPPORTUNITIES
Authors: Sharma Pankaj , Parkash Ravi
Published: April 2018

The paper analyze the role of rural tourism for the development of rural areas, The study analyzed how the different types of tourist product diversifications influence the development possibilities of studied rural areas in India. The government should sponsor private Sector to promote tourism in rural areas. For upgrading the rural tourism government requires to understand the rural location, demography, socio-culture, financial and political background of that area. How we can involve the rural citizens to improve their socio-economic condition. The objective of this paper is to present an update on rural tourism expansion and development in India. Rural tourism is rising in terms of number of visitors and the government of India should focus on it as an engine of growth
Regression Analysis for the Identification of RAPD Markers Linked To Drought Tolerance in Sorghum
Authors: Cahyaningrum Paramita , Taryono T. , Rimbawanto Anto
Published: April 2018

Sorghum (Sorghum bicolor) can actually withstand in dry or drought condition better than other crops,therefore it can be grown at different agroclimatic conditions and its product can be used for different purposessuch as food, feed and industrial raw material. However at severe condition, the productivity will also dropdrastically. The aim of this research was to identify RAPD marker linked to the drought tolerance. In thisresearch, varieties of sorghum used as research materials were Durra, Zhengzu, the mutants of Durra andZhengzu (from 300 Gy gamma radiation) B-100 and Zh-30, and the F2 seeds from Zh-30 x B-100 and B-100 xZh-30. Drought screening was carried out using 0.3 % KI during sorghum vegetative stage. DNA extractionwas done using a modified CTAB method. PCR was carried out for RAPD analysis. PCR amplification productswere scored and analyzed using SAS program. The result showed that potassium iodide can be used fordrought screening during the vegetative stage and regression analysis using the logistic method can be usedto identify RAPD markers that is linked to drought tolerance in sorghum. The logistic analysis showed thatband A8-480 was linked to drought tolerance in sorghum.
Selection of Phalaenopsis amabilis L. Blume Orchid Resistance to Hygromycin
Authors: Mercuriani Ixora Sartika , Purwantoro Aziz , Moeljopawiro Sukarti , Jang Seonghoe , Semiarti Endang
Published: April 2018

Examination of Phalaenopsis amabilis orchid resistance to hygromycin antibiotic is an important step to doprior to Agrobacterium-mediated genetic transformation in this orchids using Hygromycin phosphotransferase(HPT) gene as a selection marker in the T-DNA that harboring a desired gene to be transfered. We exposedthe plant on hygromycin containing medium. The experiment was conducted using 6 weeks old P. amabilisprotocorms. These protocorms were subcultured onto NP medium supplemented with various concentrationof Hygromycin (0, 5, 10, 20, 1nd 40 mg/l). The number of survival protocorms were examined every week for4 weeks after subcultured (WAS). The resistancy of hygromycin was calculated as ratio of death protocormsper total protocorms). The result showed that 10 mg/l hygromycin with 1 weeks of application caused deathclose to LD 50. This data indicate that P. amabilis resistance to hygromycin treatment on the appropriateconcentration 10 mg/l, and this concentration can be used for other purposes in orchid system.
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate
Authors: Sulistyaningsih Erma , Moeljopawiro Sukarti , Subandono Jarot , Artama Wayan T.
Published: April 2018

Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and itwas used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii localisolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was thendigesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1-encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein.Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed100% homologous.Keywords: GRA1 protein, Toxoplasma gondii, tachyzoite, cloning, cDNA
Genetic Analysis of Glycoprotein Gene of Indonesian Rabies Virus
Authors: Susetya Heru , Naoto Ito , Sugiyama Makoto , Minamoto Nobuyuki
Published: April 2018

The amino acid sequences of the Glycoprotein gene (G gene) of field rabies virus SN01-23 from Indonesiawas determined. This isolate showed homology of 93% in the ectodomain of the Glycoprotein gene to that of theRC-HL strain, which is used for production of animal vaccine in Japan. The high identity in the ectodomainbetween this field isolate and strain RC-HL suggest that the rabies animal vaccine used in Japan will be effectivefor rabies street viruses in Indonesia. Result of phylogenetic analysis using the nucleotide sequences of the Ggenes of rabies street viruses showed that SN01-23 from Indonesia is more closely related to a rabies virus fromChina than to viruses from Thailand and Malaysia. This genetic data and historical background suggest thatrabies viruses in China had been transferred to Indonesia through dogs brought by humans migrating from Chinato Indonesia.Keywords : Rabies virus, Glycoprotein gene, Ectodomain, Phylogenetic analysis
Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells
Authors: Fahrudin Mokhamad , Karja Ni Wayan Kurniani , Suzuki Tatsuyuki
Published: April 2018

This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effectson the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somaticcells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved andserum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclearenvelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. Therewas no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicatingthat either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasmenvironments in similar pattern. Although the fusion rate was not significantly different among the groups, theproportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%)was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos thatdeveloped to the morula and blastocyst stages were not significantly different among the groups. Results of thesestudies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluentsomatic cells, showed similar developmental competence to the blastocyst stage.Keywords: nuclear transplantation technique-somatic cells-nucleus swelling
Analysis of Htra Gene from Zebrafish (Danio Rerio)
Authors: Murwantoko M. , Oka Chio , Kawaichi Masashi
Published: April 2018

HtrA which is characterized by the combination of a trypsin-like catalytic domain with at least one C-terminalPDZ domain is a highly conserved family of serine proteases found in a wide range of organisms. However theidentified HtrA family numbers varies among spesies, for example the number of mammalian, Eschericia coli,fruit fly-HtrA family are 4, 3 and 1 gene respectively. One gene is predicted exist in zebrafish. Since no completeinformation available on zebrafish HtrA, in this paper zebrafish HtrA (zHtrA) gene was analyzed. The zHtrA isbelonged to HtrA1 member and predicted encodes 478 amino acids with a signal peptide, a IGF binding domain,a Kazal-type inhibitor domain in the up stream of HtrA-bacterial homolog. At the amino acid sequence the zHtrA1showed the 69%, 69%, 68%, 54% and 54% with the rat HtrA1, mouse HtrA1, human HtrA1, human HtrA3 andmouse HtrA4 respectively. The zHtrA1 is firstly expressed at 60 hpf and mainly in the vertebral rudiments in thetail region.
Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell)
Authors: Haryanto Aris , Kann Michael
Published: April 2018

The application of fluorescent proteins as expression markers and protein fusion partners has provedimmensely valuable for resolving the organization of biological events in living cells. EGFP and DsRed2 arecommonly fluorescent marker protein which is used for biotechnology and cell biology research. The presentstudy was designed to identify the expression vector that suitable to ligate with DNA encoding HBV coreprotein for intracellular localization study in hepatocyte cell, which were expressed as fusion proteins. We alsocompared and quantified the expressed fluorescent protein which predominantly localized in the cellcompartment. The results indicated that DsRed2 shown as less than ideal for intracellular localization study ofthan EGFP, because of its tetrameric structure of the fluorescent protein and when fused to a protein of interest,the fusion protein often forms aggregates in the living cells. In contrast, EGFP fluorescent protein shown a muchhigher proportion of cytoplasmic localization, thus being more suitable for analysis of intracellular localizationthan DsRed2 fluorescent protein. EGFP fluorescent protein is also capable to produce a strong green fluorescencewhen excited by blue light, without any exogenously added substrate or cofactor, events inside living cell canthus be visualized in a non-invasive way. Based on our present quantitative data and some reasons above shownthat EGFP is more suitable than DsRed2 as a fluorescent marker protein for intracellular localization study intoHuH-7 cell.Keywords: EGFP, DsRed2 fluorescent protein , HuH-7 cell, HBV, intracellular localization
Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR
Authors: Wijayanti Nastiti , Nirwati Hera , Wibawa Tri , Haryanto Aris , Sutaryo S.
Published: April 2018

world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detectingdengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primersthat conserve for several Flavivirus. The results showed that in 12 suspect patients, 100% were positive for Flavivirusand there are some genotypic variation among them, that indicated by several RT-PCR products higher than 511 bp,the expected product for RT-PCR. Further assay was performed to clarify the presence and serotypes of dengue virususing multiplex nested PCR. Serotyping results indicated that 83,3% of samples can be confirmed for dengue virus.Among the dengue virus positive 16,7 % are dengue-2, 16.7 % are dengue-3, and the most common 50% are dengue-4,whereas dengue-1 were not found among the patients. The combination of RT-PCR and multiplex nested PCR assaycan be used for rapid analysis dengue samples in early phase which is potentially useful for clinical, epidemiologyand also evolutionary studies.Key words: Flavivirus, dengue virus, serotype, RT-PCR, multiplex nested PCR
Biochemival Characterization of an Antibactrial Glycoprotein from Achatina fulica ferussac Snail Mucus Local Isolate and Their Implication on Bacterial Dental Infection
Authors: Berniyanti Titiek , Waskito Edy Bagus , Suwarno S.
Published: April 2018

Snails crawl over a variety of potentially contaminated surfaces and their foot is the primary site of entry forpathogens, parasites and a range of opportunistic organisms, so it is a little wonder that they must have a defensivesystem to protect them. The mucus secreted on the body surfaces of mollusks is known to play crucial role inlocomotion, feeding, osmoregulation, reproduction and protection of epithelial surfaces. The snail mucus alsocontains Glycoaminoglycans (GAGs) which are complex polysaccharides that participate in the regulation ofphysiological processes through the interactions with a wide variety of proteins. GAGs, such as heparin, serve as keyto biological response modifiers, in example for acting asa a target for pathogen and parasitic factors for attachment,invasion, and immune system.For years, it has been known that the mucus secretions from snails Achatina fulica ferussac local isolate can be usedas a medication, and even empirically it is used to treat infected teeth tahat is suffered by people in rural area. Theantibacterial factor was surveyed in the aqueous extract and the mucin fraction of snail Achatina fulica ferussac, andthey exhibited positive antibacterial for Gram-positive, Escherichia coli and Gram negative, Streptococcus mutans. Inthe following study, it has been proved that an antibacterial content in the mucus was a Glycoprotein. It wascomposed of two subunits of Molecular Weight (MW) 71-73 kDa. The GelCode Glycoprotein Staining Kit detectedglycoprotein sugar moieties in polyacrylamide gel and on nitrocellulose membrane, while the glycoproteincarbohydrate estimation kit detected glycoprotein and estimated carbohydrate content. The glycoprotein contentwas 4.537 ± 0.876 for carbohydrate and 6.420 ± 1.242 for protein.Keywords : characterization, glycoprotein, Achatina fullica Ferussac snail mucus, galur Jawa, antibacterialfactor
Alternative Oxidase (AtAox) c78s Mutant Expression at Escherichia coli (SASX41DB)
Authors: Djajanegara Ira
Published: April 2018

Alternative oxidase (AOX) is the terminal oxidase operating in the mitochondrial electron transport chain. Theenzyme is activated by organic acid such as pyruvate and by reduction process. Based on sequences alignment ofalternative oxidase gene (Aox) found in several organisms, there are 2 conserved cysteine residues. In order toinvestigate the importance of those cysteine residues on the activity of AOX, mutation at cysteine residue number 78of Aox gene isolated from Arabidopsis thaliana (AtAox) was conducted. Cysteine at position number 78 was changedinto serine and the c78s mutant was expressed in Escherichia coli strain SASX41DB. This particular E. coli strain isunable to grow aerobically unless transformed with Arabidopsis Aox gene (AtAox). Expression studies on c78smutant showed that this mutant cannot be oxididized and can not be activated by pyruvic acid. This mutant isacivated by succinate instead of pyruvate. Mutation at cysteine closer to the N residue is affecting both organic acidand redox activation. Therefore, it is concluded that cysteine residue closer to the N residue is the site for bothactivation by pyruvate as well as activation by reduction process.Keywords : Alternative oxidase, site-directed mutation, SASx41DB, cysteine residues
The Beliefs of Teachers, Students, and Parents on the Importance of Learning Second Language at a Young Age
Authors: Dogan Ozlem
Published: April 2018

There are so many factors which affect the learners when learning second language. Age, motivation of the learners, the ability to acquire language, cognitive development as the factors affect learning second language. When learners start at a young age the other factors are affected positively. The researcher aimed to search the importance of age when learning foreign language. She made a questionnaire with 52 volunteers; 26 of them are students at Foreign Language Department in Uludag University, 26 of them are parents. And she also interviewed with teachers. She carried out this study by collecting the qualitative and quantitative data. The research has shown that “Age” is important factor when learning second language. Person who learned second language at a young age has a lot of advantages in long term. The researcher chose this subject because she thought that learning second language at a young age is easier inasmuch as language acquisition is available. She wanted to learn thoughts of parents, students in Foreign Language Departments, teachers. She revealed the thought of “the younger the better” by making interview with teachers and making a questionnaire with parents and students in Foreign Language Departments. The researcher corroborate her theory by looking at these results.
Precision and Work Fluctuations in Gaussian Battery Charging
Authors: Friis Nicolai , Huber Marcus
Published: April 2018

One of the most fundamental tasks in quantum thermodynamics is extracting energy from one system and subsequently storing this energy in an appropriate battery. Both of these steps, work extraction and charging, can be viewed as cyclic Hamiltonian processes acting on individual quantum systems. Interestingly, so-called passive states exist, whose energy cannot be lowered by unitary operations, but it is safe to assume that the energy of any not fully charged battery may be increased unitarily. However, unitaries raising the average energy by the same amount may differ in qualities such as their precision, fluctuations, and charging power. Moreover, some unitaries may be extremely difficult to realize in practice. It is hence of crucial importance to understand the qualities that can be expected from practically implementable transformations. Here, we consider the limitations on charging batteries when restricting to the feasibly realizable family of Gaussian unitaries. We derive optimal protocols for general unitary operations as well as for the restriction to easier implementable Gaussian unitaries. We find that practical Gaussian battery charging, while performing significantly less well than is possible in principle, still offers asymptotically vanishing relative charge variances and fluctuations.
Ritual, Embodiment and the Paradox of Doing the Laundry
Authors: McCabe Maryann
Published: April 2018

Paradox often provides a starting point for cultural analysis of consumer behavior. The paradox of the laundry in which mothers find the laundry a boring and repetitive task yet hesitate to relinquish the chore to others is examined through the embodied experience of women’s laundry rituals. Performance of the ritual generates feelings of competence in cleaning clothes to an absolute standard of cleanliness and feelings of caring, nurturance and love of family. For mothers, the ritual goal of cultivating subjectivity in children about presentation of self to the world depends on drawers full of clean clothes. Laundry rituals are transformative because they ignite and renew emotions relating to a perceived parental role. This article discusses implementation of anthropological practice in terms of incorporating ethnographic research findings into advertising communications. In the implementation process, agency is key in bridging discourse of mothers and discourse of advertising and in producing culture.
The Use of Anthropology as a Management Framework in a Corporate Context: Review and Prospects
Authors: Preister Kevin
Published: April 2018

Many anthropologists work with corporations to understand and work effectively with communities impacted by their activities. This paper presents a Social Ecology model oriented to citizen empowerment and impact management, identifying concepts that shape ethnography into a management framework: informal networks and routines, human geography, and citizen issue management. Affecting corporate behavior can be accomplished in phases, from community assessment, to issue management, to ethnographic training programs. Their applications are described in a subdivision development, an electric utility project, and a program of paradigm change in the right-of-way, infrastructure and energy industries.
Nefropatía C1q, Reporte de caso
Authors: Lemus Barrios Gustavo Alexis , Henao-Velásquez Carlos
Published: April 2018

La nefropatía C1q es una rara glomerulonefritis caracterizada por depósitos mesangiales difusos, dominantes o codominantes de la fracción C1q de complemento, en ausencia de un perfil clínico e inmunológico de lupus eritematoso sistémico (LES). Describimos el caso de una mujer de 27 años de edad que presentó síndrome nefrótico de causa no clara, en quien se descartó  LES y síndrome antifosfolípido (SAF), pero que tenía una biopsia renal compatible con esta enfermedad. En la inmunofluorescencia se observaron depósitos mesangiales de C1q ++, IgG ++, IgM++ C3++,  y ausencia de depósitos de IgA y C4.  Tras el diagnóstico se  instauró manejo inmunosupresor logrando estabilidad clínica. Se realiza descripción del caso y  revisión de la literatura  de esta rara causa de síndrome nefrótico.
Heat transfer performance of film condensation created by forced flow
Authors: Mosaad El-Sayed
Published: April 2018

In this work, condensate film on a vertical wall cooled on the external side by forced flow is analysed as a conjugate heat transfer problem. The treated case is that the condensate film and forced flow boundary-layer are in a parallel-flow arrangement. The mass, momentum and energy boundary-layer equations of the condensate film and forced flow are set in a dimensionless form to generalize the model. The parameters affecting the thermal communication between the condensate film and the forced flow are defined from the analysis. These parameters explain the relative impact of the three involved thermal resistances of solid wall, forced convection and film condensation on the local and mean Nusselt number. The study shows that the Nusselt number predicted by the present conjugate model is different from that predicted by a Nusselt-type model.
Ética se aprende na escola?
Authors: Burmester Haino
Published: April 2018

*
Trabajo sexual en Córdoba: biopolítica, sexo y cuerpos. La ley de Profilaxis de las enfermedades venéreas: el papel de la prensa y del Estado en la construcción de los cuerpos de las prostitutas en 1938
Authors: Busquier Lucía María
Published: April 2018

Este trabajo analiza qué tipo de cuerpo se construyó sobre la figura de las prostitutas a partir del rol del Estado y el discurso de la prensa, tomando como punto de partida la sanción de la Ley Nacional 12.331: Profilaxis de las enfermedades venéreas, en Córdoba en 1938. Para ello, se estudian las reglamentaciones de dicha ley, cómo se llevó a cabo el control estatal sobre esos cuerpos, qué tipo de instituciones fueron creadas para tal fin y qué lugar jugó la prensa en el cumplimiento de estas normativas. Como propuesta metodológica, se realiza un análisis cualitativo de fuentes primarias centrado en dos periódicos de la época: La Voz del Interior y  Los Principios; así como en la Ley Nacional 12.331: Profilaxis de las enfermedades venéreas. En una segunda instancia, se exponen los principales puntos de debate sobre esta actividad en la actualidad.