bluuebunny/crossref_metadata_2025 huggingface dataset:
Interactions Among Nerve Regeneration, Angiogenesis, and the Immune Response Immediately After Sciatic Nerve Crush Injury in Sprague-Dawley Rats
Authors: He Bo , Pang Vincent , Liu Xiangxia , Xu Shuqia , Zhang Yi , Djuanda David , Wu Guanggeng , Xu Yangbin , Zhu Zhaowei
Published: October 2021

To preliminarily explore the primary changes in the expression of genes involved in peripheral nerve processes, namely, regeneration, angiogenesis, and the immune response, and to identify important molecular therapeutic targets, 45 Sprague-Dawley (SD) rats were randomly divided into a control group and an injury group. In the injury group, tissue samples were collected at 4 and 7 days after the injury for next-generation sequencing (NGS) analysis combined with gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Venn diagram construction to identify the differentially expressed mRNAs (DEmRNAs) associated with regeneration, angiogenesis, and the immune response of the nerve. The expression of genes in the distal and proximal ends of the injured nerve after injury was analyzed by qRT-PCR. NGS revealed that compared with the control group, the injury group had 4020 DEmRNAs 4 days after injury and 3278 DEmRNAs 7 days after injury. A bioinformatics analysis showed that C-C chemokine receptor type 5 (CCR5), Thy1 cell surface antigen (Thy1), Notch homolog 1 (Notch1), and semaphorin 4A (Sema4A) were all associated with regeneration, angiogenesis, and the immune response of the nerve at both 4 and 7 days after injury, but qPCR revealed no significant difference in the expression of Thy1 (P = 0.29) or Sema4A (P = 0.82) in the proximal end, whereas a significant difference was observed in CCR5 and Notch1 (P < 0.05). The trend in the Notch1 change was basically consistent with the RNA-seq result after injury, which implied its indispensable role during endothelial cell proliferation and migration, macrophage recruitment, and neurotrophic factor secretion.
Visual Snow Syndrome as a Network Disorder: A Systematic Review
Authors: Klein Antonia , Schankin Christoph J.
Published: October 2021

Aim:By reviewing the existing clinical studies about visual snow (VS) as a symptom or as part of visual snow syndrome (VSS), we aim at improving our understanding of VSS being a network disorder.

Background:Patients with VSS suffer from a continuous visual disturbance resembling the view of a badly tuned analog television (i.e., VS) and other visual, as well as non-visual symptoms. These symptoms can persist over years and often strongly impact the quality of life. The exact prevalence is still unknown, but up to 2.2% of the population could be affected. Presently, there is no established treatment, and the underlying pathophysiology is unknown. In recent years, there have been several approaches to identify the brain areas involved and their interplay to explain the complex presentation.

Methods:We collected the clinical and paraclinical evidence from the currently published original studies on VS and its syndrome by searching PubMed and Google Scholar for the term visual snow. We included original studies in English or German and excluded all reviews, case reports that did not add new information to the topic of this review, and articles that were not retrievable in PubMed or Google Scholar. We grouped the studies according to the methods that were used.

Results:Fifty-three studies were found for this review. In VSS, the clinical spectrum includes additional visual disturbances such as excessive floaters, palinopsia, nyctalopia, photophobia, and entoptic phenomena. There is also an association with other perceptual and affective disorders as well as cognitive symptoms. The studies that have been included in this review demonstrate structural, functional, and metabolic alterations in the primary and/or secondary visual areas of the brain. Beyond that, results indicate a disruption in the pre-cortical visual pathways and large-scale networks including the default mode network and the salience network.

Discussion:The combination of the clinical picture and widespread functional and structural alterations in visual and extra-visual areas indicates that the VSS is a network disorder. The involvement of pre-cortical visual structures and attentional networks might result in an impairment of “filtering” and prioritizing stimuli as top-down process with subsequent excessive activation of the visual cortices when exposed to irrelevant external and internal stimuli. Limitations of the existing literature are that not all authors used the ICHD-3 definition of the VSS. Some were referring to the symptom VS, and in many cases, the control groups were not matched for migraine or migraine aura.
Behavioral Measures of Cochlear Gain Reduction Depend on Precursor Frequency, Bandwidth, and Level
Authors: DeRoy Milvae Kristina , Strickland Elizabeth A.
Published: October 2021

Sensory systems adjust to the environment to maintain sensitivity to change. In the auditory system, the medial olivocochlear reflex (MOCR) is a known physiological mechanism capable of such adjustment. The MOCR provides efferent feedback between the brainstem and cochlea, reducing cochlear gain in response to sound. The perceptual effects of the MOCR are not well understood, such as how gain reduction depends on elicitor characteristics in human listeners. Physiological and behavioral data suggest that ipsilateral MOCR tuning is only slightly broader than it is for afferent fibers, and that the fibers feed back to the frequency region of the cochlea that stimulated them. However, some otoacoustic emission (OAE) data suggest that noise is a more effective elicitor than would be consistent with sharp tuning, and that a broad region of the cochlea may be involved in elicitation. If the elicitor is processed in a cochlear channel centered at the signal frequency, the growth of gain reduction with elicitor level would be expected to depend on the frequency content of the elicitor. In the current study, the effects of the frequency content and level of a preceding sound (called a precursor) on signal threshold was examined. The results show that signal threshold increased with increasing precursor level at a shallower slope for a tonal precursor at the signal frequency than for a tonal precursor nearly an octave below the signal frequency. A broadband noise was only slightly more effective than a tone at the signal frequency, with a relatively shallow slope similar to that of the tonal precursor at the signal frequency. Overall, these results suggest that the excitation at the signal cochlear place, regardless of elicitor frequency, determines the magnitude of ipsilateral cochlear gain reduction, and that it increases with elicitor level.
Relationship Between Subjective Ratings of Answers and Behavioral and Autonomic Nervous Activities During Creative Problem-Solving via Online Conversation
Authors: Numata Takashi , Kotani Kiyoshi , Sato Hiroki
Published: October 2021

Creative problem solving has been important for the advent of new technologies. In this study, we hypothesized that subjective ratings of answers should be useful for evaluating the answer quality in creative problem solving. To test this hypothesis and extract objective indicators of the subjective ratings of answers, we evaluated the relationship between subjective ratings of task performance and behavioral and autonomic nervous activities during a creative problem-solving task performed via online conversation. The task involved an answerer and a supporter, and in the experiment, each pair performed 10 trials. The trials were categorized as highly or lowly rated according to the answerer’s confidence in the answer. The task performance and behavioral and autonomic nervous activities were then compared between these categories of trials. Behavioral activity was evaluated via movements and speech activities, while for autonomic nervous activity, sympathetic nervous activity (SNA) was evaluated via skin conductance. The task performance was significantly better in the highly rated trials, whereas there were no significant differences in the behavioral activities between the highly and lowly rated trials. Moreover, in the highly rated trials, the skin conductance of the answerer was significantly high, whereas that of the supporter was significantly low. The results support the hypothesis and suggest that contrasting differences in SNA between an answerer and a supporter are indicators of the subjective ratings of answers in creative problem solving.
Can Cultured Meat Be an Alternative to Farm Animal Production for a Sustainable and Healthier Lifestyle?
Authors: Munteanu Camelia , Mireşan Vioara , Răducu Camelia , Ihuţ Andrada , Uiuiu Paul , Pop Daria , Neacşu Alexandra , Cenariu Mihai , Groza Ioan
Published: October 2021

Producing animal proteins requires large areas of agricultural land and is a major source of greenhouse gases. Cellular agriculture, especially cultured meat, could be a potential alternative for the environment and human health. It enables meat and other agricultural products to be grown from cells in a bioreactor without being taken from farm animals. This paper aims at an interdisciplinary review of literature focusing on potential benefits and risks associated with cultured meat. To achieve this goal, several international databases and governmental projects were thoroughly analyzed using keywords and phrases with specialty terms. This is a growing scientific domain, which has generated a series of debates regarding its potential effects. On the one hand the potential of beneficial effects is the reduction of agricultural land usage, pollution and the improvement of human health. Other authors question if cultured meat could be a sustainable alternative for reducing gas emissions. Interestingly, the energy used for cultured meat could be higher, due to the replacement of some biological functions, by technological processes. For potential effects to turn into results, a realistic understanding of the technology involved and more experimental studies are required.
Targeting the Tumor Extracellular Matrix by the Natural Molecule 4-Methylumbelliferone: A Complementary and Alternative Cancer Therapeutic Strategy
Authors: Vitale Daiana L. , Icardi Antonella , Rosales Paolo , Spinelli Fiorella M. , Sevic Ina , Alaniz Laura D.
Published: October 2021

In antineoplastic therapy, one of the challenges is to adjust the treatment to the needs of each patient and reduce the toxicity caused by conventional antitumor strategies. It has been demonstrated that natural products with antitumoral properties are less toxic than chemotherapy and radiotherapy. Also, using already developed drugs allows developing substantially less costly methods for the discovery of new treatments than traditional drug development. Candidate molecules proposed for drug repositioning include 4-methylumbelliferone (4-MU), an orally available dietetic product, derivative of coumarin and mainly found in the plant family Umbelliferae or Apiaceae. 4-MU specifically inhibits the synthesis of glycosaminoglycan hyaluronan (HA), which is its main mechanism of action. This agent reduces the availability of HA substrates and inhibits the activity of different HA synthases. However, an effect independent of HA synthesis has also been observed. 4-MU acts as an inhibitor of tumor growth in different types of cancer. Particularly, 4-MU acts on the proliferation, migration and invasion abilities of tumor cells and inhibits the progression of cancer stem cells and the development of drug resistance. In addition, the effect of 4-MU impacts not only on tumor cells, but also on other components of the tumor microenvironment. Specifically, 4-MU can potentially act on immune, fibroblast and endothelial cells, and pro-tumor processes such as angiogenesis. Most of these effects are consistent with the altered functions of HA during tumor progression and can be interrupted by the action of 4-MU. While the potential advantage of 4-MU as an adjunct in cancer therapy could improve therapeutic efficacy and reduce toxicities of other antitumoral agents, the greatest challenge is the lack of scientific evidence to support its approval. Therefore, crucial human clinical studies have yet to be done to respond to this need. Here, we discuss and review the possible applications of 4-MU as an adjunct in conventional antineoplastic therapies, to achieve greater therapeutic success. We also describe the main proposed mechanisms of action that promote an increase in the efficacy of conventional antineoplastic strategies in different types of cancer and prospects that promote 4-MU repositioning and application in cancer therapy.
Diagnostic and Prognostic Potential of 18F-FET PET in the Differential Diagnosis of Glioma Recurrence and Treatment-Induced Changes After Chemoradiation Therapy
Authors: Celli Monica , Caroli Paola , Amadori Elena , Arpa Donatella , Gurrieri Lorena , Ghigi Giulia , Cenni Patrizia , Paganelli Giovanni , Matteucci Federica
Published: October 2021

Background

MRI-based differential diagnosis of glioma recurrence (GR) and treatment-induced changes (TICs) remain elusive in up to 30% of treated glioma patients. We aimed to determine 18F-FET PET diagnostic performance in this clinical scenario, its outcome dependency on established prognostic factors, optimal 18F-FET semi-quantitative thresholds, and whether 18F-FET parameters may instantly predict progression-free survival (PFS) and overall survival (OS).

Methods

We retrospectively analyzed 45 glioma patients treated with chemoradiation therapy (32 males; mean age: 51 years, glioma grade: n=26 WHO4; n=15 WHO3; n=4 WHO2) who underwent 18F-FET PET to resolve differential diagnosis of GR and TICs raised by MRI performed in the preceding 2 weeks and depicting any of the following changes in their radiation field: volumetric increase of contrast-enhancing lesions; new contrast-enhancing lesion; significant increase in T2/FLAIR non-enhancing lesion without reducing corticosteroids. 18F-FET PET outcome relied on evaluation of maximum tumor-to-brain ratio (TBRmax), time-to-peak (TTP), and time-activity curve pattern (TAC). Metabolic tumor volume (MTV) and total tumor metabolism (TTM) were calculated for prognostic purposes. Standard of reference was repeat MRI performed 4–6 weeks after the previous MRI. Non-parametric statistics tested 18F-FET-based parameters for dependency on established prognostic markers. ROC curve analysis determined optimal cutoff values for 18F-FET semi-quantitative parameters. 18F-FET parameters and prognostic factors were evaluated for PFS and OS by Kaplan-Meier, univariate, and multivariate analyses.

Results

18F-FET PET sensitivity, specificity, positive predictive value, negative predictive value were 86.2, 81.3, 89.3, 76.5%, respectively; higher diagnostic accuracy was yielded in IDH-wild-type glioma patients compared to IDH-mutant glioma patients (sensitivity: 81.8 versus 88.9%; specificity: 80.8 versus 81.8%). KPS was the only prognostic factor differing according to 18F-FET PET outcome (negative versus positive). Optimal 18F-FET cutoff values for GR were TBRmax ≥ 2.1, SUVmax ≥ 3.5, and TTP ≤ 29 min. PFS differed based on 18F-FET outcome and related metrics and according to KPS; a different OS was observed according to KPS only. On multivariate analysis, 18F-FET PET outcome was the only significant PFS factor; KPS and age the only significant OS factors.

Conclusion

18F-FET PET demonstrated good diagnostic performance. 18F-FET PET outcome and metrics were significantly predictive only for PFS.
The Role of ARID1A in Tumors: Tumor Initiation or Tumor Suppression?
Authors: Xu Shouying , Tang Chao
Published: October 2021

Genes encoding subunits of SWItch/Sucrose Non-Fermenting (SWI/SNF) chromatin remodeling complexes are collectively mutated in 20% of all human cancers, among which the AT-rich interacting domain−containing protein 1A (ARID1A, also known as BAF250a, B120, C1orf4, Osa1) that encodes protein ARID1A is the most frequently mutated, and mutations in ARID1A have been found in various types of cancer. ARID1A is thought to play a significant role both in tumor initiation and in tumor suppression, which is highly dependent upon context. Recent molecular mechanistic research has revealed that ARID1A participates in tumor progression through its effects on control of cell cycle, modulation of cellular functions such as EMT, and regulation of various signaling pathways. In this review, we synthesize a mechanistic understanding of the role of ARID1A in human tumor initiation as well as in tumor suppression and further discuss the implications of these new discoveries for potential cancer intervention. We also highlight the mechanisms by which mutations affecting the subunits in SWI/SNF complexes promote cancer.
Scorpion Venom Heat-Resistant Peptide Attenuates Microglia Activation and Neuroinflammation
Authors: Wu Xue-Fei , Li Chun , Yang Guang , Wang Ying-Zi , Peng Yan , Zhu Dan-Dan , Sui Ao-Ran , Wu Qiong , Li Qi-Fa , Wang Bin , Li Na , Zhang Yue , Ge Bi-Ying , Zhao Jie , Li Shao
Published: October 2021

Background: Intervention of neuroinflammation in central nervous system (CNS) represents a potential therapeutic strategy for a host of brain disorders. The scorpion Buthus martensii Karsch (BmK) and its venom have long been used in the Orient to treat inflammation-related diseases such as rhumatoid arthritis and chronic pain. Scorpion venom heat-resistant peptide (SVHRP), a component from BmK venom, has been shown to reduce seizure susceptibility in a rat epileptic model and protect against cerebral ischemia-reperfusion injury. As neuroinflammation has been implicated in chronic neuronal hyperexcitability, epileptogenesis and cerebral ischemia-reperfusion injury, the present study aimed to investigate whether SVHRP has anti-inflammatory property in brain.

Methods: An animal model of neuroinflammation induced by lipopolysacchride (LPS) injection was employed to investigate the effect of SVHRP (125 µg/kg, intraperitoneal injection) on inflammagen-induced expression of pro-inflammatory factors and microglia activation. The effect of SVHRP (2–20 μg/ml) on neuroinflammation was further investigated in primary brain cell cultures containing microglia as well as the immortalized BV2 microglia culture stimulated with LPS. Real-time quantitative PCR were used to measure mRNA levels of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in hippocampus of animals. Protein levels of TNF-α, iNOS, P65 subunit of nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs) were examined by ELISA or western blot. Microglia morphology in animal hippocampus or cell cultures and cellular distribution of p65 were shown by immunostaining.

Results: Morphological study demonstrated that activation of microglia, the main component that mediates the neuroinflammatory process, was inhibited by SVHRP in both LPS mouse and cellular model. Our results also showed dramatic increases in the expression of iNOS and TNF-α in hippocampus of LPS-injected mice, which was significantly attenuated by SVHRP treatment. In vitro results showed that SVHRP attenuated LPS-elicited expression of iNOS and TNF-α in different cultures without cell toxicity, which might be attributed to suppression of NF-κB and MAPK pathways by SVHRP.

Conclusion: Our study demonstrates that SVHRP is able to inhibit neuroinflammation and microglia activation, which may underlie the therapeutic effects of BmK-derived materials, suggesting that BmK venom could be a potential source for CNS drug development.
Investigation of the Possible Allostery of Koumine Extracted From Gelsemium elegans Benth. And Analgesic Mechanism Associated With Neurosteroids
Authors: Xiong Bojun , You Wenbing , Luo Yufei , Jin Guilin , Wu Minxia , Xu Ying , Yang Jian , Huang Huihui , Yu Changxi
Published: October 2021

Translocator protein 18 kDa (TSPO) is an evolutionarily conserved 5-transmembrane domain protein, and has been considered as an important therapeutic target for the treatment of pain. We have recently reported the in vitro and in vivo pharmacological characterization of koumine as a TSPO positive allosteric modulator (PAM), more precisely ago-PAM. However, the probe dependence in the allostery of koumine is an important question to resolve, and the possible analgesic mechanism of koumine remains to be clarified. Here, we report the in vivo evaluation of the allostery of koumine when orthosteric ligand PK11195 was used and preliminarily explore the possible analgesic mechanism of koumine associated with neurosteroids. We find that koumine is an ago-PAM of the PK11195-mediated analgesic effect at TSPO, and the analgesic mechanism of this TSPO ago-PAM may be associated with neurosteroids as the analgesic effects of koumine in the formalin-induced inflammatory pain model and chronic constriction injury-induced neuropathic pain model can be antagonized by neurosteroid synthesis inhibitor aminoglutethimide. Although our results cannot fully clarify the allosteric modulatory effect of koumine, it further prove the allostery in TSPO and provide a solid foundation for koumine to be used as a new clinical candidate drug to treat pain.
Translation-Invariant Bipolarons and Charge Density Waves in High-Temperature Superconductors
Authors: Lakhno Victor D.
Published: October 2021

A correlation is established between the theories of superconductivity based on the concept of charge density waves (CDWs) and the translation invariant (TI) bipolaron theory. It is shown that CDWs are originated from TI-bipolaron states in the pseudogap phase due to the Kohn anomaly and form a pair density wave (PDW) for wave vectors corresponding to nesting. Emerging in the pseudogap phase, CDWs coexist with superconductivity at temperatures below those of superconducting transition, while their wave amplitudes decrease as a Bose condensate is formed from TI bipolarons, vanishing at zero temperature.
Confined Monolayer Ice Between CaF2 (111) and Graphene: Structure and Stability
Authors: Li Shi-Qi , Qiu Shi , Liu Hongsheng , Chen Maodu , Gao Junfeng
Published: October 2021

Water monolayer can form in layered confined systems. Here, CaF2 (111) and graphene are chosen as modeling systems to explore the structure and stability of confined monolayer water. First, water molecules tend to intercalate into a confined space between graphene and CaF2, rather than on a bare surface of graphene. Water molecules can move fast in the confined space due to a low diffusion barrier. These water molecules are likely to aggregate together, forming monolayer ice. Four ice phases including ice II, ice III, ice IV, and ice Ih are compared in this confined system. Intriguingly, all the ice phases undergo very small deformation, indicating the 2D monolayer ice can be stable in the CaF2–graphene–confined system. Beyond, projected band structures are also plotted to understand the electronic behavior of these confined ice phases. Nearly all the bands originated from confined ices are flat and locate about 2–3 eV below the Fermi level. Binding energy calculations suggest that the stability sequence in this confined system as follows: Ih-up ≈ Ih-down ≈ II < IV < III. Our results bring new insights into the formation of water monolayer production in such a confined condition.
Dispersion Characteristic of Spatiotemporal Sharply Autofocused Vector Airy-Circular Airy Gaussian Vortex Wave Packets
Authors: Li Dong , Chen Xin , Wei Chengquan , Li Peng , Zhao Jianlin
Published: October 2021

The spatiotemporal vector Airy-Circular Airy Gaussian vortex wave packet is constructed by solving the (3 + 1)D Schrodinger equation in free space. The wave packet can simultaneously autofocus in space and time by setting the appropriate initial pulse velocityυand the initial position of the main lobeT0. This kind of wave packet has low intensity before focusing, but the intensity at focus is about 80 times of the initial plane intensity. Our results may have potential applications in particle manipulation, laser processing, and other fields. Furthermore, the influence of the third-order dispersion coefficient on the evolution trajectory, the focus position, and the main peak intensity at the focus of the focusing pulse vector field is analyzed. The results show that the change of the initial velocity, the initial position, and the third-order dispersion coefficient can accurately control the evolution trajectory and the focus position, while the main peak intensity at the focus can only be controlled by adjusting the third-order dispersion coefficient. This means that the pulse vector light field can be manipulated precisely for precise processing by adjusting the third-order dispersion effect.
A Dual-Wideband Balanced Bandpass Filter Based on Branch-Line Structure With Controllable Common-Mode Suppression
Authors: Ren Baoping , Liu Xinlei , Guan Xuehui , Xu Mengrou , Zhang Zhi-Chong
Published: October 2021

In this paper, a novel dual-wideband balanced bandpass filter (BPF) based on branch-line structure is proposed. For analysis, the equivalent circuits of differential-mode (DM) and common-mode (CM) of the filter are built based on the even- and odd-mode method. With a proper synthesis design of DM bisection, dual passbands with a multi-order filtering response can be obtained. Additionally, three open-circuited stubs are centrally loaded on the CM bisection and six controllable transmission zeros are therefore generated. Thus, two stopbands are formed and then a favorable CM suppression within DM passbands is obtained. For demonstration, a third-order dual-wideband balanced BPF is designed with two passbands operating at 2.54 and 4.62 GHz. Good agreement between the simulated results and measured results is obtained, which verifies the validity of the proposed design method.
Genetic Dissection Uncovers Genome-Wide Marker-Trait Associations for Plant Growth, Yield, and Yield-Related Traits Under Varying Nitrogen Levels in Nested Synthetic Wheat Introgression Libraries
Authors: Sandhu Nitika , Kaur Amandeep , Sethi Mehak , Kaur Satinder , Varinderpal-Singh  , Sharma Achla , Bentley Alison R. , Barsby Tina , Chhuneja Parveen
Published: October 2021

Nitrogen is one of the most important macronutrients for crop growth and metabolism. To identify marker-trait associations for complex nitrogen use efficiency (NUE)-related agronomic traits, field experiments were conducted on nested synthetic wheat introgression libraries at three nitrogen input levels across two seasons. The introgression libraries were genotyped using the 35K Axiom® Wheat Breeder's Array and genetic diversity and population structure were examined. Significant phenotypic variation was observed across genotypes, treatments, and their interactions across seasons for all the 22 traits measured. Significant positive correlations were observed among grain yield and yield-attributing traits and root traits. Across seasons, a total of 233 marker-trait associations (MTAs) associated with fifteen traits of interest at different levels of nitrogen (N0, N60, and N120) were detected using 9,474 genome-wide single nucleotide polymorphism (SNP) markers. Of these, 45 MTAs for 10 traits in the N0 treatment, 100 MTAs for 11 traits in the N60 treatment, and 88 MTAs for 11 traits in the N120 treatment were detected. We identified putative candidate genes underlying the significant MTAs which were associated directly or indirectly with various biological processes, cellular component organization, and molecular functions involving improved plant growth and grain yield. In addition, the top 10 lines based on N response and grain yield across seasons and treatments were identified. The identification and introgression of superior alleles/donors improving the NUE while maintaining grain yield may open new avenues in designing next generation nitrogen-efficient high-yielding wheat varieties.
Precise Characterization and Tracking of Stably Inherited Artificial Minichromosomes Made by Telomere-Mediated Chromosome Truncation in Brassica napus
Authors: Yin Xiangzhen , Zhang Yingxin , Chen Yuhong , Wang Jingqiao , Wang Richard R.-C. , Fan Chengming , Hu Zanmin
Published: October 2021

Plant artificial minichromosomes are the next-generation technology for plant genetic engineering and represent an independent platform for expressing foreign genes and the tools for studying the structure and function of chromosomes. Minichromosomes have been successfully produced by telomere-mediated chromosome truncation in several plants. However, previous studies have primarily focused on the construction and rough characterization of minichromosomes, while the development of stably inherited minichromosomes and their precise characterization and tracking over different generations have rarely been demonstrated. In this study, a 0.35-kb direct repeat of the Arabidopsis telomeric sequence was transformed into Brassica napus to produce artificial minichromosomes, which were analyzed by multifluorescence in situ hybridization (multi-FISH), Southern hybridization, and primer extension telomere rapid amplification (PETRA). The stably inherited minichromosomes C2 and C4 were developed by crossing transgenic plants with wild-type plants and then selfing the hybrids. Notably, two truncation sites on chromosomes C2 and C4, respectively, were identified by resequencing; thus, the artificial minichromosomes were tracked over different generations with insertion site-specific PCR. This study provided two stably inherited minichromosomes in oilseed rape and describes approaches to precisely characterize the truncation position and track the minichromosomes in offspring through multi-FISH, genome resequencing, and insertion site-specific PCR.
Institutional Pressure and Green Product Success: The Role of Green Transformational Leadership, Green Innovation, and Green Brand Image
Authors: Zhou Jian , Sawyer Lucinda , Safi Adnan
Published: October 2021

Rapid economic growth has led to economic activities which have caused extensive environmental damage to the planet. Companies have sought to adapt their business methods to reduce their carbon footprint in order to meet regulations, satisfy consumer preferences and keep up with changing societal expectations. The relationship between institutional pressure and green product performance will be an important issue in corporate green management. This article looked through the lens of green innovation and explored the moderating role of green brand image between green product innovation and new green product success. Utilising the data of 243 managers in Mainland China, structural equation modelling results found that institutional pressure is positively correlated to green transformational leadership, green transformational leadership is positively correlated to green process innovation, green process innovation is positively correlated to green product innovation, green product innovation is positively correlated with new green product success, green brand image moderates the relationship between green product innovation and new green product performance. The research results provide theoretical and practical implications for enterprises to relieve institutional pressure and build specific green competitive advantages.
The Production of Relatives in Mandarin Children With Specific Language Impairment–From the Perspective of Edge Feature Underspecification Hypothesis
Authors: Wang Haiyan , Yu Haopeng
Published: October 2021

This paper is a first attempt to investigate the production of Relative Clauses (RCs) in Mandarin children with Specific Language Impairment (SLI) (aged 4; 5 to 6; 0) and their typically developing (TD) peers. The data from a preference choice task suggested that (i) Children with SLI performed better on the subject-gapped than object-gapped RC; (ii) Children with SLI performed substantially worse than their TD peers on the RCs production; (iii) Children with SLI were more inclined to omitting the complementizer and using simple sentences and sentence fragments as avoidance strategies. The Edge Feature Underspecification Hypothesis may explain not only the asymmetry of production seen in children with SLI, but also the presence of errors and avoidance strategies used by this population in the task.
Musicianship Influences Language Effect on Musical Pitch Perception
Authors: Choi William
Published: October 2021

Given its practical implications, the effect of musicianship on language learning has been vastly researched. Interestingly, growing evidence also suggests that language experience can facilitate music perception. However, the precise nature of this facilitation is not fully understood. To address this research gap, I investigated the interactive effect of language and musicianship on musical pitch and rhythmic perception. Cantonese and English listeners, each divided into musician and non-musician groups, completed the Musical Ear Test and the Raven’s 2 Progressive Matrices. Essentially, an interactive effect of language and musicianship was found on musical pitch but not rhythmic perception. Consistent with previous studies, Cantonese language experience appeared to facilitate musical pitch perception. However, this facilitatory effect was only present among the non-musicians. Among the musicians, Cantonese language experience did not offer any perceptual advantage. The above findings reflect that musicianship influences the effect of language on musical pitch perception. Together with the previous findings, the new findings offer two theoretical implications for the OPERA hypothesis—bi-directionality and mechanisms through which language experience and musicianship interact in different domains.
Assessing the Role of Gaze Tracking in Optimizing Humans-In-The-Loop Telerobotic Operation Using Multimodal Feedback
Authors: Bolarinwa Joseph , Eimontaite Iveta , Mitchell Tom , Dogramadzi Sanja , Caleb-Solly Praminda
Published: October 2021

A key challenge in achieving effective robot teleoperation is minimizing teleoperators’ cognitive workload and fatigue. We set out to investigate the extent to which gaze tracking data can reveal how teleoperators interact with a system. In this study, we present an analysis of gaze tracking, captured as participants completed a multi-stage task: grasping and emptying the contents of a jar into a container. The task was repeated with different combinations of visual, haptic, and verbal feedback. Our aim was to determine if teleoperation workload can be inferred by combining the gaze duration, fixation count, task completion time, and complexity of robot motion (measured as the sum of robot joint steps) at different stages of the task. Visual information of the robot workspace was captured using four cameras, positioned to capture the robot workspace from different angles. These camera views (aerial, right, eye-level, and left) were displayed through four quadrants (top-left, top-right, bottom-left, and bottom-right quadrants) of participants’ video feedback computer screen, respectively. We found that the gaze duration and the fixation count were highly dependent on the stage of the task and the feedback scenario utilized. The results revealed that combining feedback modalities reduced the cognitive workload (inferred by investigating the correlation between gaze duration, fixation count, task completion time, success or failure of task completion, and robot gripper trajectories), particularly in the task stages that require more precision. There was a significant positive correlation between gaze duration and complexity of robot joint movements. Participants’ gaze outside the areas of interest (distractions) was not influenced by feedback scenarios. A learning effect was observed in the use of the controller for all participants as they repeated the task with different feedback combination scenarios. To design a system for teleoperation, applicable in healthcare, we found that the analysis of teleoperators’ gaze can help understand how teleoperators interact with the system, hence making it possible to develop the system from the teleoperators’ stand point.
Real-Time Shape Estimation for Concentric Tube Continuum Robots with a Single Force/Torque Sensor
Authors: Donat Heiko , Gu Jiecong , Steil Jochen J.
Published: October 2021

Shape-sensing in real-time is a key requirement for the development of advanced algorithms for concentric tube continuum robots when safe interaction with the environment is important e.g., for path planning, advanced control, and human-machine interaction. We propose a real-time shape-estimation algorithm for concentric tube continuum robots based on the force-torque information measured at the tubes’ basis. It extends a shape estimation algorithm for elastic rods based on discrete Kirchhoff rod theory. For simplicity and efficiency of calculation, we combine it with a model under piece-wise constant curvature assumption, in which we model a concentric tube continuum robot as a combination of segments of planar constant curvatures lying on different equilibrium planes. We evaluate our approach for a single and two combined additively manufactured tubes and achieve an estimation frequency of 333 Hz for two combined tubes with a mean deviation along the backbone of the tubes of 1.91–5.22 mm.
Risk Reduction and Productivity Increase Through Integrating Arachis pintoi in Cattle Production Systems in the Colombian Orinoquía
Authors: Enciso Valencia Karen Johanna , Rincón Castillo Álvaro , Ruden Daniel Alejandro , Burkart Stefan
Published: October 2021

In many parts of the foothills of the Orinoquía region of Colombia, cattle production takes place on poorly drained soils. The region is dominated by extensive grazing systems of Brachiaira humidicola cv. Humidicola, a grass with high adaptation potential under temporal waterlogging conditions. Inadequate management practices and low soil fertility result in degradation, however, with important negative effects on pasture productivity and the quality and provision of (soil) ecosystem services–a situation that is likely to worsen in the near future due to climate change. Against this background, AGROSAVIA (Corporación Colombiana de Investigación Agropecuaria) selected Arachis pintoi CIAT 22160 cv. Centauro (Centauro) as a promising alternative for the sustainable intensification of livestock production and rehabilitation of degraded areas. This study assesses dual-purpose milk production in the foothills of the Colombian Orinoquía from an economic perspective. We compare two production systems: the Centauro–Brachiaira humidicola cv. Humidicola association (new system) and Brachiaira humidicola cv. Humidicola as a monoculture (traditional system). We used cashflow and risk assessment models to estimate economic indicators. The projections for economic returns consider changes in forage characteristics under regional climate change scenarios RCP (2.6, 8.5). The LIFE-SIM model was used to simulate dairy production. Results show that the inclusion of Centauro has the potential to increase animal productivity and profitability under different market scenarios. The impact of climatic variables on forage production is considerable in both climate change scenarios. Both total area and potential distribution of Centauro could change, and biomass production could decline. Brachiaira humidicola cv. Humidicola showed better persistence due to higher nitrogen levels in soil when grown in association with Centauro. The legume also provides a number of ecosystem services, such as improving soil structure and composition, and also contributes to reducing greenhouse gas emissions. This helps to improve the adaptation and mitigation capacity of the system.
Comparison of Micro-Percutaneous and Mini-Percutaneous Nephrolithotomy in the Treatment of Renal Stones: A Systematic Review and Meta-Analysis
Authors: Gao Xiaoshuai , Wang Wei , Peng Liao , Di Xingpeng , Xiao Kaiwen , Chen Jixiang , Jin Tao
Published: October 2021

Background: To assess the efficacy and safety of micro-percutaneous nephrolithotomy (Microperc) and mini-percutaneous nephrolithotomy (Miniperc) in the treatment of moderately sized renal stones.

Methods: Literature search of PubMed, Web of Science, and Embase was performed prior to January 2021. We used odds ratios (OR) and weighted mean difference (WMD) for dichotomous variables and continuous variables, respectively. Results were pooled using Review Manager version 5.3 software.

Results: A total of six studies involving 291 Microperc and 328 Miniperc cases was included. The overall stone-free rate (SFR) of Microperc was 87.29% (254/291), while the SFR of Miniperc was 86.59% (284/328). Microperc was associated with lower hemoglobin drop (WMD: −0.98; P = 0.03) and higher renal colic requiring D-J stent insertion (OR: 3.49; P = 0.01). No significant differences existed between Microperc and Miniperc with respect to SFR (OR: 1.10; P = 0.69), urinary tract infection (OR: 0.38; P = 0.18), operative time (WMD: −5.76; P = 0.62), and hospital stay time (WMD: −1.04; P = 0.07).

Conclusions: Our meta-analysis demonstrated that Microperc could produce an SFR that was comparable with that of Miniperc. Microperc was associated with lower hemoglobin drop, while Miniperc was associated with lower renal colic rates. In addition, the operation time and hospital stay time for both these procedures were similar.
Companion Animal Ownership and Mood States of University Students Majoring in Animal Sciences during the COVID-19 Pandemic in Japan
Authors: Namekata Daiki , Yamamoto Mariko
Published: October 2021

COVID-19 caused great difficulties in many people’s daily lives, including university students in Japan. This study examined whether living with companion animals and attachment to companion animals influence the moods of university students. Students answered a questionnaire, including demographic data, companion animal ownership, attachment to their companion animals, perceived difficulties from COVID-19, and Profile of Mood States 2 (POMS2) results. A total of 180 students answered the questionnaire. Stepwise multiple regression analyses were conducted to identify factors related to the total mood disturbance (TMD) score of the POMS2. In the regression model, perceived difficulties from COVID-19 and having a companion animal and a strong attachment to their companion animals were significantly correlated with TMD and served as the predictor variables. The first variable was positively related to TMD, whereas companion animal ownership with high attachment to their companion animals was negatively related to TMD. This finding indicated that companion animal ownership with high attachment to their companion animals would relate to a positive mood in university students majoring in animal sciences during the COVID-19 pandemic. However, because of the limited population in size and by the students’ major, the results need to be interpreted as a possible effect of companion animals, and not as conclusive evidence to support the effects of animals.
Nifedipine Potentiates Susceptibility of Salmonella Typhimurium to Different Classes of Antibiotics
Authors: Haschka David , Grander Manuel , Eibensteiner Johannes , Dichtl Stefanie , Koppelstätter Sabine , Weiss Günter
Published: October 2021

The calcium channel blocker nifedipine induces cellular iron export, thereby limiting the availability of the essential nutrient iron for intracellular pathogens, resulting in bacteriostatic activity. To study if nifedipine may exert a synergistic anti-microbial activity when combined with antibiotics, we used the mouse macrophage cell line RAW267.4, infected with the intracellular bacterium Salmonella Typhimurium, and exposed the cells to varying concentrations of nifedipine and/or ampicillin, azithromycin and ceftriaxone. We observed a significant additive effect of nifedipine in combination with various antibiotics, which was not observed when using Salmonella, with defects in iron uptake. Of interest, increasing intracellular iron levels increased the bacterial resistance to treatment with antibiotics or nifedipine or their combination. We further showed that nifedipine increases the expression of the siderophore-binding peptide lipocalin-2 and promotes iron storage within ferritin, where the metal is less accessible for bacteria. Our data provide evidence for an additive effect of nifedipine with conventional antibiotics against Salmonella, which is partly linked to reduced bacterial access to iron.
Synergy between Indoloquinolines and Ciprofloxacin: An Antibiofilm Strategy against Pseudomonas aeruginosa
Authors: Charpentier Emilie , Doudet Ludovic , Allart-Simon Ingrid , Colin Marius , Gangloff Sophie C. , Gérard Stéphane , Reffuveille Fany
Published: October 2021

Antibiotic treatments can participate in the formation of bacterial biofilm in case of under dosage. The interest of indoloquinoline scaffold for drug discovery incited us to study the preparation of new indolo [2,3-b]quinoline derivatives by a domino radical process. We tested the effect of two different “indoloquinoline” molecules (Indol-1 and Indol-2) without antimicrobial activity, in addition to ciprofloxacin, on biofilm formation thanks to crystal violet staining and enumeration of adhered bacteria. This association of ciprofloxacin and Indol-1 or Indol-2 attenuated the formation of biofilm up to almost 80% compared to ciprofloxacin alone, or even prevented the presence of adhered bacteria. In conclusion, these data prove that the association of non-antimicrobial molecules with an antibiotic can be a solution to fight against biofilm and antibiotic resistance emergence.
Antimicrobial Resistance of Listeria monocytogenes Strains Isolated from Humans, Animals, and Food Products in Russia in 1950–1980, 2000–2005, and 2018–2021
Authors: Andriyanov Pavel A. , Zhurilov Pavel A. , Liskova Elena A. , Karpova Tatyana I. , Sokolova Elena V. , Yushina Yulia K. , Zaiko Elena V. , Bataeva Dagmara S. , Voronina Olga L. , Psareva Ekaterina K. , Tartakovsky Igor S. , Kolbasov Denis V. , Ermolaeva Svetlana A.
Published: October 2021

Susceptibility of 117 L. monocytogenes strains isolated during three time periods (1950–1980; 2000–2005, and 2018–2021) to 23 antibiotics was tested by the disk diffusion method. All strains were sensitive to aminoglycosides (gentamicin, kanamycin, neomycin, streptomycin), glycopeptides (vancomycin and teicoplanin), clarithromycin, levofloxacin, amoxicillin/clavulanic acid, and trimethoprim/sulfamethoxazole. Resistance to clindamycin was observed in 35.5% of strains. Resistance to carbapenems, imipenem and meropenem was found in 4% and 5% of strains, respectively. Resistance to erythromycin, penicillin G, trimethoprim, and ciprofloxacin was found in 4%, 3%, 3%, and 2.5% of strains, respectively. Resistance to tylosin, ampicillin, enrofloxacin, linezolid, chloramphenicol, and tetracycline was found in less than 2%. Three strains with multiple antibiotic resistance and 12 strains with resistance to two antibiotics were revealed. Comparison of strains isolated in different time periods showed that the percentage of resistant strains was the lowest among strains isolated before 1980, and no strains with multiple antibiotic resistance were found among them. Statistical analysis demonstrated that the temporal evolution of resistance in L. monocytogenes has an antibiotic-specific character. While resistance to some antibiotics such as ampicillin and penicillin G has gradually decreased in the population, resistance to other antibiotics acquired by particular strains in recent years has not been accompanied by changes in resistance of other strains.