bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of vitamin C supplementation on the oxidative status of rat liver
Authors: Djurasevic S.F. , Djordjevic Jelena , Drenca Tamara , Jasnic N. , Cvijic Gordana
Published: June 2008

Rats have the ability to synthesize vitamin C in the liver. In the present work, we tested whether its additional intake improves antioxidative protection of the tissue. Thus, we studied the liver?s oxidative status in rats given two supplements of ascorbic acid over a four-week period of time. Our results confirmed that the additional intake of ascorbate improves the liver?s antioxidative defense in a dose-dependent manner. The explanation for the disproportion between the ratio of employed doses of vitamin C and their effects on the studied parameters probably lies in the mechanism of tissue accumulation of ascorbate and balance of its alimentary and endogenous availability.
Desmid flora (Chlorophyta, Zygnematophyceae) of the Danube in the province of Vojvodina (Northern Serbia)
Authors: Stamenkovic M. , Cvijan M.
Published: June 2008

This paper presents results of a detailed investigation into the qualitative composition of the desmid flora of the Danube performed from April of 2002 to May of 2003. A total of 70 desmid taxa belonging to four genera were recorded. Forty-seven taxa are new to the Vojvodina stretch of the Danube, while Cosmarium kjellmanii, Staurastrum bloklandiae, and S. smithii are taxa new to the algal flora of Serbia. Qualitative dominance of desmid taxa typical of alkaline and eutrophic ecosystems was observed. The qualitative composition of planktonic taxa, their quantitative composition, and regular seasonal dynamics of the desmid community were analyzed in the relation to physico-chemical characteristics of the water. Water quality was analyzed on the basis of desmids, which are bioindicators. The partly unexpected presence of certain desmid taxa, i.e., several typical acidophilous species, alpine forms, and/or taxa characteristic of oligotrophic water was recorded.
Fungal colonization of air-conditioning systems
Authors: Ljaljevic-Grbic Milica , Vukojevic Jelena , Stupar M.
Published: June 2008

Fungi have been implicated as quantitatively the most important bioaerosol component of indoor air associated with contaminated air-conditioning systems. rarely, indoor fungi may cause human infections, but more commonly allergenic responses ranging from pneumonitis to asthma-like symptoms. From all air conditioner filters analyzed, 16 fungal taxa were isolated and identified. Aspergillus fumigatus causes more lethal infections worldwide than any other mold. Air-conditioning filters that adsorb moisture and volatile organics appear to provide suitable substrates for fungal colonization. It is important to stress that fungal colonization of air-conditioning systems should not be ignored, especially in hospital environments.
Genetic variability within Serbian populations of the rare and endangered pottioid moss Hilpertia velenovskyi (Schiffn.) Zander inferred by isozyme analyses
Authors: Sabovljevic M. , Sabovljevic Aneta , Radulovic J. , Dragicevic Ivana
Published: June 2008

Genetic variability within Serbian populations of the pottioid moss Hilpertia velenovskyi (Schiffn.) Zander was studied. Eight populations of this rare and endangered moss species were chosen for peroxidase isozyme analyses. From the data obtained, it can be inferred that four haplotypes are present among H. velenovskyi populations in Serbia, the Banat population being distinctive from all others.
Monitoring of the “lichen desert” in the Belgrade area (1980/81, 1991 and 2007)
Authors: Cvijan M. , Subakov-Simic Gordana , Krizmanic Jelena
Published: June 2008

This paper treats long-term changes in area of the ?lichen desert?, i.e., the region characterized (especially in the winter period) by average daily SO2 concentration exceeding 150?g/m3 of air, the maximum allowable concentration (MAC) for populated regions. Comparison of results obtained in 1980/81 and 1991 indicates that the ?lichen desert? expanded (from 12 to about 13 km2) as a consequence of deterioration of the air quality in the Belgrade area. Comparing the results obtained in 1991 with investigations carried out in 2007, we found that ?lichen desert? shrank substantially in the Belgrade area to a current size of about 11 km2. The new boundaries of the ?lichen desert? are presented, together with the main reasons for its shrinkage in the Belgrade area during the last 16 years and predictions of further alterations in the future.
Activities of antioxidative enzymes during Chenopodium rubrum L. Ontogenesis in vitro
Authors: Mitrovic Aleksandra , Bogdanovic Jelena
Published: June 2008

For the short-day plant Chenopodium rubrum, a 14 h/10 h photoperiod is inductive for flowering, while continuous light (CL) is noninductive. Plants of one group were grown continuously under an inductive photoperiod, while in the other group flowering induction was delayed by 17 days of CL in order to separate on the time scale different developmental phases in plants of the same age. Regardless of the photoperiodic conditions the plants were exposed to, seed maturation occurred in 10 weeks. Activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) were determined in different phases of development (vegetative growth, flowering, seed development, and maturation). The activities of antioxidative enzymes depended on both the phase of development and the photoperiod. In plants grown continuously under an inductive photoperiod, high CAT and POD activities were detected at the time of flowering and decreased during seed development and maturation. In plants in which flowering induction was delayed by 17 days of CL, the activities of POD and SOD were lowest in the vegetative phase of development and attained maximum values in the phase of seed maturation. In both groups of plants, the highest CAT activity was measured at the time of flowering.
Chemical composition and antifungal activity of Salvia sclarea (Lamiaceae) essential oil
Authors: Dzamic Ana , Sokovic Marina , Ristic M. , Grujic-Jovanovic Slavica , Vukojevic Jelena , Marin P.D.
Published: June 2008

Clary sage (Salvia sclarea L.) is native to Southern Europe and is cultivated worldwide. The essential oil of clary sage was analyzed as a potential antifungal agent. The main compounds in the oil were linalyl acetate (52.83%) and linalool (18.18%). Food poisoning agents, spoilage fungi, and plant and animal pathogens were among the tested fungal species. The microdilution method was used to establish minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC). The commercial antimycotic bifonazole was used as a control. A concentration of 25 ?l/ml showed fungicidal activity against Aspergillus, Penicillium, and Fusarium species and Trichoderma viride. For the species Mucor mucedo and Aspergillus viride, the MFC was 15?l/ml; for Candida albicans, it was 10 ?l/ml, as in the case of bifonazole. Fungistatic and fungicidal activities of the oil against Cladosporium cladosporioides and Trichophyton menthagrophytes were recorded at concentrations of 2.5 ?l/ml and 5 ?l/ml. The most sensitive micromycetes were Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi, and Phoma macdonaldii, where a concentration of 2.5 ?l/ml was lethal.
Lead uptake, tolerance, and accumulation exhibited by the plants Urtica dioica and Sedum spectabile in contaminated soil without additives
Authors: Grubor Milena
Published: June 2008

Specimens of Urtica dioica and Sedum spectabile collected from plants growing at uncontaminated sites were transplanted in Pb-contaminated soil without additives (EDTA, HEDTA) to identify their natural potential for hyper-tolerance and hyperaccumulation of lead. The total content of Pb in the plants was determined by atomic spectroscopy. Our research showed that the concentrated toxic levels of lead (Pb) in Sedum spectabile and Urtica dioica were about 100 or more times higher than those of non-accumulator plants. It can be concluded that these plants have a high natural potential for hypertolerance and hyperaccumulation of lead, since they can hyperaccumulate it without addition of any chelating compounds (EDTA, HEDTA) to enhance lead uptake. This makes them very promising plants for use in phytoremediation of Pb-contaminated sites.
Flora of the city of Podgorica, Montenegro: Taxonomic analysis
Authors: Stesevic Danijela , Jovanovic S.
Published: June 2008

In this paper we present the taxonomic segment of a floristic study undertaken in the city of Podgorica in the period of 2002-2007. The check-list of spontaneously growing vascular plants includes 1227 species and subspecies belonging to 545 genera and 118 families. The dominant families are Poaceae (11.7%), asteraceae (11.2%) and Fabaceae (9.2%). The most abundant genera are Trifolium (2.1%), Euphorbia (1.5%), Carex (1.5%), Bromus (1.3%) and Vicia (1.2%). Analysis of the flora of Podgorica in comparison with some other european cities showed that the flora of Podgorica is most similar to that of Rome (Q/S= 0,7).
Use of Bermuda grass [Cynodon dactylon (L.) Pers.] on ash and slag dumps of thermoelectric power plants
Authors: Stavretovic N. , Manjasek Suzana
Published: June 2008

Biological recultivation is conducted regularly on ash and slag dumps of thermoelectric power plants. New methods and new plant species for use in the process of recultivation are being investigated by experts the world over. Bermuda grass (Cynodon dactylon) represents a potentially valuable species that should become an ingredient of sowing mixtures used in the process of revitalisation of ash and slag dumps of thermoelectric power plants.
Results of the 2006 Sava survey: Aquatic macroinvertebrates
Authors: Paunovic M.M. , Borkovic Slavica , Pavlovic S.Z. , Saicic Zorica , Cakic P.D.
Published: June 2008

The paper presents some results of the 2006 Sava survey. The investigation was carried out at four locations along 188 km of the Serbian stretch of the Sava River (206 km of the river belongs to Serbia). Among other things (physical and chemical properties of water and sediment, phytoplankton, and phytobenthos), the study included investigation of aquatic macroinvertebrates. Sixty-two taxa were identified in this typical lowland river. Mollusks and oligochaetes were the most diverse groups of macroinvertebrates. Our results support the hypothesis that the Sava River is an important bio-invasion trajectory, a part of the Southern Invasive Corridor of Europe. Five alien macroinvertebrate taxa were identified, some of which (Corbicula fluminea, Branchyura sowerbyi, and Anodonta woodiana) were found to be important components of the community.
Nest occupation and prey grabbing by saker falcon (Falco cherrug) on power lines in the province of Vojvodina (Serbia)
Authors: Puzovic S.
Published: June 2008

Research on nest occupation and prey grabbing by saker falcon (Falco cherrug) on power lines in Vojvodina (Serbia) was done in the period from 1986 to 2004. During three specially analyzed periods, saker falcon took the nests of raven (Corvus corax) in 91% of a total of 22 cases of nest occupation, and those of hooded crow (Corvus corone cornix) in only 9%. Saker falcon regularly grabs prey from different birds that occasionally or constantly spend time around power lines [Kestrel (Falco tinnunculus), hobby (Falco subbuteo), hooded crow (Corvus corone cornix), jack-daw (Corvus monedula), marsh harrier (Circus aeruginosus), hen harrier (Circus cyaneus), buzzard (Buteo buteo), and raven (Corvus corax)]. One year a studied pair of saker falcons on a power line in Donji Srem, Serbia grabbed prey from five different species of birds. Out of a total of 40 cases of prey grabbing in the period from January to December, as much 70% of the grabbed prey was taken from kestrel (Falco tinnunculus). During the winter and early spring, prey was grabbed predominantly by males; after May, prey was sometimes grabbed by females as well. Most of the grabbed prey was common vole (Microtus arvalis).
Sexual differences in size and shape of the Mosor rock lizard [Dinarolacerta mosorensis (Kolombatovic, 1886)] (squamata: lacertidae): A case study of the Lovcen mountain population (Montenegro)
Authors: Ljubisavljevic Katarina , Polovic Lidija , Ivanovic Ana
Published: June 2008

Sexual differences in size and shape of the Mosor rock lizard, Dinarolacerta mosorensis (Kolombatovic, 1886), from Lovcen Mountain (Montenegro) were examined on the basis of the intersex variation pattern of nine morphometric, eight pholidotic, and four qualitative traits. Sexual dimorphism was apparent for all morphometric characters except snout-vent length, while scalation and dorsal pattern exhibited small differences between sexes. The value of the sexual size difference (SSD) index based on snout-vent length was 1.028. The sex-specific allometric slopes for head dimensions and interlimb distance significantly diverged. Head dimensions, especially head height, showed strong positive allometry in males, while interlimb distance was the only character which showed positive allometry in females. Generally, males had significantly greater body size than females. This was true of all body measurements except interlimb distance. The influence of sexual and natural selection on the examined traits is discussed.
The genus Ruteria roudier, 1954 (Curculionidae) in Serbia
Authors: Pesic Snezana , Ilic N.
Published: June 2008

The presence of Ruteria graeca (Caldara, 1973) and R. hypocrita (Boheman, 1837) (93 and 215 specimens respectively) was confirmed among other adult soil weevil material collected at 24 localities on the territory of Serbia between 1995 and 2003 for the most part using pitfall traps. Ruteria hypocrita was much more frequent. In both species, males were dominant (36.55 and 37.21%, respectively). Biogeographically, the new Ruteria graeca findings are an impor- tant supplement completing the picture of the mosaic distribution of this species, endemic to the Balkan Peninsula. Until now, the given species was completely unknown in Serbia, i.e., in the central part of the Balkan Peninsula. Our data show a new northern boundary of its distribution. In addition, we provide ecological details about the finding places of both species. Briefly, different deciduous and mixed deciduous-coniferous woods at various altitudes and on different geological substrates are host ecosystems for Ruteria.
Heteromurus (Verhoeffiella) Anagastumensis n. Sp. (Collembola, entomobryidae), a new cave springtail from Montenegro
Authors: Lucic L.R. , Tomic V.T. , Brajkovic M.M. , Curcic S.B.
Published: June 2008

A thorough analysis of a specimen of the collembolan family Entomobryidae from a cave in Montenegro has yielded a species new to science: Heteromurus (Verhoeffiella) anagastumensis n. sp. This is the first known cave-dwelling member of the subgenus Verhoeffiella Absolon in Montenegro. Heteromurus (V.) anagastumensis n. sp. is described, illustrated, and diagnosed; it is a phenetically close congener of H. (V.) longicornis (Absolon) from Herzegovina. Some biogeographical and evolutionary characteristics of this new Verhoeffiella species are discussed in the light of the origin of the North Mediterranean fauna.
Neobisium ninae n. Sp. (Neobisiidae, pseudoscorpiones), a new endemic cave pseudoscorpion from Montenegro
Authors: Curcic S.B. , Dimitrijevic R.N. , Tomic V.T.
Published: June 2008

A new cave-dwelling species of pseudoscorpion belonging to the family Neobisiidae - Neobisium ninae n. sp. - is described from the Pecina u Dubokom Potoku Cave in the village of Donje Bisevo, near Rozaje in Eastern Montenegro. A diagnosis of the new species is presented. This taxon is a relict and endemic to the area studied. Additionally, some taxonomic and biogeographical traits of the new species are discussed in the light of the evolution of the karst relief of the Balkan Peninsula.
On two new species of pseudoscorpions from the dinaric karst
Authors: Curcic B.P.M. , Dimitrijevic R.N. , Radja T. , Radja Biljana
Published: June 2008

A careful analysis of samples of pseudoscorpions (Neobisiidae, Pseudoscorpiones) from two epigean habitats, one near Split (Croatia) and the other on Mt. Orjen (Montenegro), has yielded two species of the genus Roncus L. Koch new to science: Roncus diocletiani n. sp. and R. orjensis n. sp. Both new species are described, thoroughly illustrated, and diagnosed. Some biogeographical and evolutionary characteristics of the two taxa are briefly discussed.
Alpha-tocopheryl succinate (α-TOS) influences cell vitality and enzyme activity in Ehrlich ascites carcinoma cells
Authors: Stankov Karmen , Bajin-Katic Katica , Stanimirov Bojan , Karadzic Dunja , Kovacevic Zoran
Published: June 2008

Background: One of the most important strategies in research and development of new anticancer agents is the tumor-specific induction of apoptosis. The effects of semisynthetic derivative of vitamin E, (?-TOS, D-?-tocopheryl succinate), appear to be largely restricted to malignant cells. Methods: We investigated the in vivo effects of intraperitoneally administered ?-TOS on vitality of Ehrlich ascites carcinoma cells (EAC) in mice, as well as the influence of ?-TOS on specific activity of enzymes involved in antioxidative mechanisms in EAC cells. Results: According to our results, the intraperitoneal application of ?-TOS induces the decrease of the EAC vitality, and the statistically significant alteration of the glutathione-dependent enzyme activity in EAC cells. Conclusion: We may conclude that ?-TOS is an important micronutrient, with significant impact on vitality and metabolism of malignant cells.
Surgical approach and results of treating two subcategories pT2 glottic carcinoma of the larynx
Authors: Jovic Rajko , Canji Karol , Mitrovic Slobodan , Kljajic Vladimir , Dragicevic Danijela
Published: June 2008

Background: T2 glottic carcinoma is a nonhomogenous localization of laryngeal carcinoma with two subcategories. The aim of the study was to retrospectively analyze the results of surgically treated pT2 glottic carcinomas and to determine the importance of subcategories of glottic carcinomas in diagnostics, surgical treatment, and patients? survival. Methods: During the period 1990-2000, 71/701 (10%) patients were diagnosed. with pT2 glottic carcinoma. All patients were treated with different surgical techniques. Results: Total laryngectomy was performed in 14/71 patients. Involvement of anterior commisure in local spreading of the cancer was found in 24/71 patients; total laryngectomy was performed in 13/24 and reconstructive surgery in 11/24. Selective resection of neck was done in 49 patients and metastases were found in 2 of these patients. Out of fifty-seven patients who had reconstructive laryngeal surgery local disease recurrence appeared in 8 patients. These 8 patients were treated with total laryngectomy. Overall 5-year survival was 90.1% with a slight difference between subcategories (89.1% subcategory I; 93.7% subcategory II) which was not statistically significant (p>0.05). Disease free 5-year survival was 83.6% with a difference between subcategories (81.1% subcategory I; 93.3% subcategory II) which was not statistically significant (p>0.05). Conclusion: Spreading of glottic carcinoma toward supraglottic and subglottic structures complicates exact preoperative evaluation of tumor size regardless to preoperative diagnostic procedures. Just owing to that, larger surgical resections performed bring more radicalness with less percentage of local recurrence and better effects on overall survival and disease free survival rate.
Radiological features of breast benign lesions in patients with hyperprolactinemia
Authors: Radojkovic Danijela , Antic Slobodan , Pesic Milica , Radojkovic Milan , Basic Dijana , Radjenovic-Petkovic Tatjana
Published: June 2008

Background: The study was conducted to determine whether prolactin (PRL) influences radiological features of benign breast lesions. Methods: During the 4-year period, we observed 50 patients with nipple discharge: 23 with normal serum prolactin levels (group 1) and 27 with hyperprolactinemia (group 2a before hyperprolactinemia treatment and group 2b after hyperprolactinemia treatment). Patients observed during this study underwent breast ultrasonography (US) and mammography. Radiological findings in group 1, group 2a and group 2b were compared. Results: The most frequent radiological findings in group 2a were dilated lactiferous ducts and cystic lesions with statistical significances p<0.001 and p<0.01 in comparison to other radiological findings. In group 1 significantly less presence of dilated lactiferous ducts was observed (p<0.05) and proportional lower frequency of solid lumps compared to group 2a. Our results showed significantly lower incidence (p<0.001) of dilated lactiferous ducts, after hyperprolactinemia treatment (group 2b). In group 2b less presence of solid lumps and enlarged axillary lymph nodes is also observed in comparison to group 2a. Frequency of cystic lesions significantly decreased (p<0.05) and pseudocysts completely withdrew in patients with normalized serum prolactin levels. Conclusion: Prolactin significantly influences radiological presentation of benign breast diseases. Treatment of hyperprolactinemia results in withdrawal of mammary duct ectasia, pseudocystic formations, cystic lesions and solid lumps. Breast ultra-sonography and mammography should be considered as routine diagnostic procedures in patients with hyperprolactinemia.
Radiation-induced peripheral neuropathies: Etiopathogenesis, risk factors, differential diagnostics, symptoms and treatment
Authors: Vasic Ljiljana
Published: June 2008

The success of radiation oncology has lead to longer patient survival. This provides a greater opportunity for radiation injuries of the peripheral nerves to develop. Peripheral neuropathy in cancer patients may result from either tumor recurrence or as a consequence of radiation therapy. Distinguishing between radiation injury and cancer disease recurrence as a cause of nerve dysfunction may be difficult. In this article the etiopathogenesis of radiation-induced peripheral neuropathies has been discussed as well as main risk factors, symptoms and method of treatment.
The frequency of sister chromatid exchange and micronuclei in evaluation of cytogenetic activity of Kombucha on human peripheral blood lymphocytes
Authors: Mrdjanovic Jasminka , Bogdanovic Gordana , Cvetkovic Dragoljub , Velicanski Aleksandra , Cetojevic-Simin Dragana
Published: June 2008

Kombucha is a refreshing beverage obtained by the fermentation of sweetened black tea with a ?tea fungus? (symbiotic culture of acetic acid bacteria and yeasts). It is consumed due to its potential beneficial effects on human health. The aim of this study was to investigate activity of Kombucha on human peripheral blood lymphocytes in vitro. We analyzed Kombucha made from different substrates: Camellia sinensis and Satureja montana, and effects of substrates alone. The frequencies of sister chromatid exchange (SCE) and micronuclei (MN) were scored as genetic endpoints and mitomycin C was used as model mutagen. Kombucha from Camellia sinensis and Camellia sinensis substrate increased frequency of MN and SCE on mitomycin C-treated and -untreated peripheral blood lymphocytes. However, Kombucha from Satureja montana reduced incidence of MN on mitomycin C-treated and -untreated peripheral blood lymphocytes, while SCE frequency was higher than control value. In our pilot study we showed for the first time that Kombucha from different substrates induced different effects on mitomycin C-treated and -untreated peripheral blood lymphocytes.
Clinical, histological and immunohistochemical features of ceruminous adenoma
Authors: Rancic Dejan , Zivic Misko , Ilic Ivan , Katic Vuka , Radovanovic Zoran
Published: June 2008

Ceruminous gland tumors are infrequent lesions of the external auditory canal (EAC). Controversy exists regarding several aspects of these tumors. In this report we presented a case of 63 years old woman with an 8-year history of a painless mass in the left EAC. Physical examination showed a skin-covered mass, occupying all its lumen. First superficial biopsy led to an initial erroneous diagnosis of papilloma. Local wide resection of the lesion was performed one month later. Histological examination revealed a lesion composed of solid nests and papillomatous and cystic glands surrounded by a hyaline sheath. Immunohistochemically, two different cell layers were demonstrated: a luminal epithelial cell layer with positive staining with cytokeratin 7 (CK7) and an outer myoepithelial cell layer, with positive staining with actin and S-100 protein. The proliferative index (Ki-67) was very low. The diagnosis was ceruminous gland adenoma. Two years after the operation, the patient is in good health and with normal ultrasound and laboratory results. We believe that conservative surgery seems to be ideal treatment in case of ceruminous adenoma.
Endoscopic argon plasma coagulation for the management of solid, centrally located lung cancer
Authors: Zaric Bojan , Canak Vukasin , Milovancev Aleksandar , Stojanovic Goran , Balaban Gordana
Published: June 2008

Argon plasma coagulation (APC) is one of the interventional pulmonology techniques primarily aimed at the treatment of hemoptysis. It represents a form of non contact electrosurgey that uses ionized argon gas in order to produce electrical current that affects soft tissues. APC is reported to be effective in the treatment of early stage lung cancer, in the treatment of benign granulation tissue surrounding tracheal stents and in palliative treatment of malignant airway obstruction. Major indication for APC is hemostasis in severe hemoptysis, it can also be used as an alternative technique for laser resection or electrocautery, in urgent removal of tumors situated in large airways. The present article reports successful use of APC in the treatment of centrally located squamous cell lung cancer that caused complete right lung atelectasis. The use of APC led to complete reexpansion of the right lung and improvement in dyspnoea and chest discomfort of the patient. Significant improvement was observed in lung function parameters and blood gas analysis. With the use of APC solid tumor was completely removed from the right main bronchus and airway integrity was restored. From this case we can conclude that APC can be safely and successfully used for urgent debulking of malignant central airway obstruction.
Thumb metastases in a patient with colorectal cancer
Authors: Nikolic Ivan , Patrnogic Aleksandar , Stojiljkovic Bratislav , Bogdanovic Bogdan , Kukic Biljana
Published: June 2008

Bone metastases from colorectal cancer are not common and most frequently are manifesting in late history of metastatic disease. We present a 67-old man who had first symptoms of metastatic disease manifested with edema and decreased mobility of thumb of right hand. Radiography showed complete osteolysis of proximal phalanx of thumb, which appeared 30 months after primary surgery for colorectal cancer. Histopathologic analysis of amputated thumb confirmed diagnosis of metastatic colorectal carcinoma and excluded osteolysis of other reasons. Metastatic changes in the first and second lumbar vertebra, which were treated with palliative radiotherapy, were confirmed by additional diagnostics.
Nikola Tesla discovered ‘very special radiation’ or x-radiation
Authors: Baltic Vladimir , Baltic Milan
Published: June 2008

Nikola Tesla was an ingenious and briliant scintifist which contributed to the science and technology prosperity of the world. Nikola Tesla discovered high frequency oscillator 1891 year, and afterwards, single electrode X-ray tube without target electrode, electron (?particles charged with electricity?), Bremsstrahlung or ?breaking radiation? or ?very special radiation? (X-radiation) in 1892 year, biological hazards of X-rays, and more about 700 inventions, and 112 patents. Tesla's research work in the field of X-rays was stopped in 1895 because burned in his laboratory. When W. C. R?ntgen 1895, recognition X-rays, Tesla restored his work on this field and sent R?ntgen a telegram with congratilations and R?ntgen shows obtained with X-ray tubes which operated with high frequency currents. Tesla discovered many secret of nature.
13C- and 1H-NMR substituent-induced chemical shifts in N(1)-(4-substituted phenyl)-3-cyano-4,6-dimethyl-2-pyridones
Authors: Marinkovic Aleksandar , Valentic Natasa , Mijin Dusan , Uscumlic Gordana , Jovanovic Bratislav
Published: June 2008

The 13C- and 1H-NMR chemical shifts of thirteen N(1)-(4-substituted phenyl)-3-cyano-4,6-dimethyl-2-pyridones were measured in deuterated dimethyl sulfoxide (DMSO-d6). The correlation analysis for the substituent-induced chemical shifts (SCS) with ?p, inductive (?I) and different scale of resonance (?R) parameters were performed using the SSP (single substituent parameter), DSP (dual substituent parameter) and DSP-NLR (dual substituent parameter-non-linear resonance) methods. The results of the calculations concerning the polar and resonance effects satisfactorily describe the substituent effects at the carbon atoms of interest. The mode of transmission of the substituent effects, both inductive and resonance, in relation to the geometry of the investigated pyridones is discussed.
Stereospecific ligands and their complexes: Synthesis and characterization of the s-cis-K[Ru(S,S-eddp)Cl2]·3H2O
Authors: Glodjovic Verica , Trifunovic Srecko
Published: June 2008

In the reaction of ruthenium(III) chloride and an edda-like ligand ethylenediamine-N,N'-di-S,S-2-propionic acid (S,S-eddp) in aqueous solution led to the formation of only one of the three possible geometrical isomers potassium- s-cis-dichlorido-(ethylenediamine-N,N'-di-S,S-2-propionato)-ruthenate(III)- -trihydrate, s-cis-K[Ru(S,S-eddp)Cl2]?3H2O. The assumed geometry of the complex was based on its electronic absorption and infrared spectra.
Partitioning of π-electrons in rings of diaza-derivatives of acenes
Authors: Stankovic Sonja , Djurdjevic Jelena , Gutman Ivan , Milentijevic Radmila
Published: June 2008

A few years ago, a method was proposed for assessing the ?-electron content (EC) of rings in heteroatom-containing benzenoid molecules. In this work, the effect of two nitrogen atoms at the opposite sides of a linear benzenoid molecule on the partitioning of its ?-electrons was investigated. The results obtained can be explained by means of resonance-theoretical arguments.
Investigation of the pitting of aluminum induced by chloride ions by holographic microphotography
Authors: Li Liang , Wang Chao , Chen Shenhao , Hou Xianmin , Yang Xuegeng
Published: June 2008

Holographic microphotography was used to investigate the dynamic processes of pitting during anodic dissolution of aluminum in a solution containing chloride ions. The induction and the follow-up propagation processes of the pitting were observed in real-time. A simple model of the propagating process of the pitting was deduced from the result of the holograms of the Al/electrolyte interface. The results prove that holographic microphotography is a useful tool to study the dynamic processes of pitting.
An indirect atomic absorption spectrometric determination of ciprofloxacin, amoxycillin and diclofenac sodium in pharmaceutical formulations
Authors: Issa Mohamed , Nejem Mahmoud , Al-Kholy Monzer , El-Abadla Said , Helles Sobhi , Saleh Amin
Published: June 2008

A highly sensitive indirect atomic absorption spectrophotometric (AAS) method has been developed for the determination of very low concentrations of ciprofloxacin, amoxycillin and diclofenac sodium. The method is based on the oxidation of these drugs with iron(III). The excess of iron(III) was extracted into diethyl ether and then the iron(II) in the aqueous layer was aspirated into an air-acetylene flame and determined by AAS. The linear concentration ranges were 25-400, 50-500 and 60-600 ng ml-1 for ciprofloxacin, amoxycillin and diclofenac sodium, respectively. The results were statistically compared with the official method using t- and f-test at p < 0.05. There were insignificant interferences from most of the excipients present. The intra- and inter-day assay coefficients of variation were less than 6.1 % and the recoveries ranged from 95 to 103 %. The method was applied for the analysis of these drug substances in their commercial pharmaceutical formulations.
Transformation of a petroleum pollutant during soil bioremediation experiments
Authors: Jovancicevic B. , Antic M. , Vrvic M. , Ilic M. , Novakovic M. , Saheed R.M. , Schwarzbauer J.
Published: June 2008

The experiment of ex situ soil bioremediation was performed at the locality of the Oil Refinery in Pancevo (alluvial formation of the Danube River, Serbia) polluted with an oil type pollutant. The experiments of biostimulation, bioventilation and reinoculation of an autochthonous microbial consortium were performed during the six-month period (May-November 2006). The changes in the quantity and composition of the pollutant, or the bioremediation effect, were monitored by analysis of the samples of the polluted soil taken in time spans of two weeks. In this way, from the beginning until the end of the experiment, 12 samples were collected and marked as P1-P12 (Pancevo 1-Pancevo 12). The results obtained showed that more significant changes in the composition of the oil pollutant occurred only during the last phases of the experiment (P8-P12). The activity of microorganisms was reflected in the increase of the quantity of polar oil fractions, mainly fatty acid fractions. In this way, the quantity of total eluate increased, and the quantity of the insoluble residue was reduced to a minimum, whereby the oil pollutant was transformed to a form that could be removed more efficiently and more completely from the soil, as a segment of the environment.
Lead and silver extraction from waste cake from hydrometallurgical zinc production
Authors: Stanojevic Dusan , Rajkovic Milos , Toskovic Dragan , Tomic Milana
Published: June 2008

This paper presents the experimental results of the extraction of lead and silver from a lead-silver waste cake obtained in the process of hydrometallurgical zinc production. While controlling the pH value, the lead-silver cake was leached at a temperature close to boiling point in different concentrations of aqueous calcium chloride solutions. The experiments were performed applying different ratios between the mass of cake and the volume of the leaching agent under different durations of the process. It was concluded that at the optimal process parameters (pH 2.0-2.5; CaCl2 concentration, 3.6 mol dm-3; temperature, 95?C; solid/liquid ratio, 1:5), the leaching efficiency of lead and silver could reach the approximate value of 94 %. Applying the same optimal process parameters, the method was applied to the leaching of a lead-silver cake in a magnesium chloride solution, but with significantly lower efficiencies. The results show that leaching of lead and silver in a calcium chloride solution could be a prospective method for increasing the recovery of lead and silver during hydrometallurgical zinc production.
Immobilization and characterization of bovine liver catalase on eggshell
Authors: Alptekin Özlem , Tükel Seyhan , Yildirim Deniz
Published: June 2008

Bovine liver catalase immobilized on eggshell particles was characterized and the reusability of the immobilized catalase was investigated in a batch type reactor. For immobilized catalase onto ground eggshell (ICATG), the optimum initial amount of catalase was 85 mg g-1 of eggshells, the optimum pH was 6.0 (75 mM citrate buffer) and the temperature was 30?C. The Vmax and Km values of ICATG were determined as 29.1?1.2 U/mg of protein and 41.9?2.7 mM, respectively. The reusability of ICATG was tested and the remaining activity of ICATG was found to be 73% of the initial activity after 80 cycles of batch operation. The amount of catalase bound onto the carrier was estimated by using the results of induced coupled plasma measurements. The catalytic efficiencies (kcat/Km) of free catalase and ICATG were found to be 1.4x106 and 2.8x103 dm3 s-1 mol-1, respectively. Catalase immobilization onto eggshell is economic and has good reusability. Hence, it can be concluded that eggshell is an efficient carrier for immobilizing catalase.
Synthesis, structural characterization and cytotoxic activity of two new organoruthenium(II) complexes
Authors: Grguric-Sipka Sanja , Alshtewi Arbi , Jeremic Dejan , Kaludjerovic Goran , Gómez-Ruiz Santiago , Zizak Zeljko , Juranic Zorica , Sabo Tibor
Published: June 2008

Two new p-cymene ruthenium(II) complexes containing as additional ligands N-methylpiperazine ([(?6-p-cymene)RuCl2(CH3NH(CH2)4NH)]PF6, complex 1) or vitamin K3-thiosemicarbazone ([(?6-p-cymene)RuCl2(K3tsc)], complex 2) were synthesized starting from [(?6-p-cymene)2RuCl2]2 and the corresponding ligand. The complexes were characterized by elemental analysis, IR, electronic absorption and NMR spectroscopy. The X-ray crystal structure determination of complex 1 revealed ?piano-stool? geometry. The differences in the cytotoxic activity of the two complexes are discussed in terms of the ligand present.
Photocatalytic degradation of phenol red using complexes of some transition metals and hydrogen peroxide
Authors: Lodha Savitri , Vaya Dipti , Ameta Rakshit , Punjabi Pinki
Published: June 2008

The photocatalytic degradation of Phenol Red was investigated using thiocyanate complexes of iron, copper, cobalt and hydrogen peroxide. The rate of photocatalytic degradation of the dye was followed spectrophotometrically. The effect of the variation of different parameters, such as pH, concentration of the complexes and dye, amount of H2O2 and light intensity on the rate of photocatalytic degradation was also studied. A tentative mechanism for the photocatalytic degradation of Phenol Red is proposed.
Coulometric-potentiometric determination of pKA of several organic bases in propylene carbonate
Authors: Jaksic Ljiljana , Dzudovic Radmila
Published: June 2008

The pKA values of protonated triethylamine, pyridine and 2,2'-dipyridyl in propylene carbonate (PC) were determined by applying the coulometric-potentiometric method and a hydrogen/palladium generator anode (H2/Pd). The investigated and reference base were titrated to 50 % with protons electro-generated from hydrogen-saturated palladium, in the presence of sodium perchlorate as the supporting electrolyte. The half-neutralization potentials E1/2(x) and E1/2(st.) of the investigated and standard base, respectively, were measured using a glass-SCE pair. The obtained pKA values were compared with those reported in the literature.
A rapid and reliable determination of doxycycline hyclate by HPLC with UV detection in pharmaceutical samples
Authors: Mitic Snezana , Miletic Gordana , Kostic Danijela , Naskovic-Ðokic Daniela , Arsic Biljana , Rasic Ivana
Published: June 2008

An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for the quantification of doxycycline hyclate in pharmaceutical samples has been developed and validated. The drug and the standard were eluted from a Lichrosorb RP-8 (250 mm x 4.6 mm, 10 mm particle size) at 20?C with a mobile phase consisting of methanol, acetonitrile and 0.010 M aqueous solution of oxalic acid (2:3:5, v/v/v). The flow rate was 1.25 ml min-1. A UV detector set at 350 nm was used to monitor the effluent. Each analysis required no longer than 4 min. The limits of detection and quantification were 1.15 and 3.84 ?g ml-1, respectively. Recoveries for different concentrations ranged from 99.58 to 101.93%.
Methods for the determination of the form of aluminium: Pseudogley soils
Authors: Mrvic Vesna , Jakovljevic Miodrag , Stevanovic Dragi , Cakmak Dragan , Zdravkovic Mirjana
Published: June 2008

Exchangeable aluminium (AlKCl) and CaCl2-extractable aluminium (AlCaCl2) have approximately the same value in the prognosis of detrimental effect on plants. Additional, more in-depth research should show which of the two applied methods of exchangeable Al determination is more suitable (after Sokolov or using aluminon). The contents of total Al and amorphous Al increase with depth and percentage clay. Crystalline Al oxides and CuCl2-extractable aluminium (AlCaCl2) and EDTA-extractable aluminium (AlEDTA) represent the more mobile reserves of Al and they depend mostly on changes in the acidity parameters of the soil. Exchangeable Al is on average 4.3-4.7 times lower than the contents of AlCaCl2 and AlEDTA, 8 times lower than crystalline Al and 35 times lower than amorphous Al. Some methods are not sufficiently selective, hence further research is required to achieve more favorable methods for the determination of the different forms of Al.
The contribution of pathologic diagnosis and research of the faculty members engaged in teaching histology and embryology to undergraduate medical studies
Authors: Somer Ljiljana , Lalosevic Dusan , Djolai Matilda
Published: June 2008

Introduction. The theoretical and practical aspects of teaching are generally defined by the curriculum, but professional experience plays an important role as well. The teaching experience of the faculty members is developed through health services, education and research. The Department of Histology and Embriology. This paper reviews the activities of the teaching staff of the Department of Histology and Embryology, pointing to the importance of pathologic diagnostics and research. It underlines the necessity of using some non-standard histologic staining methods on some tissues, organs and pathogens, as well as the results of experimental studies of the endocrine system. The experimental tumor models are used to demonstrate the interdependence of the endocrine and immune systems. Modalities and possibilities of regeneration of the digestive tract and connective tissue mucosa are explained. This paper also deals with histologic criteria used for assessment of fetal age and in the diagnosis of developmental malformations. Linear and stereologic methods, used in quantification of normal morphology, the degree of pathologic changes, and regeneration of normal structures in the courses of and after therapy, are given. The authors particularly emphasized the importance of abovementioned activities in the teaching of Histology and Embryology to undergraduate students.
Critical periods during development of the dentate nucleus and their clinical significance
Authors: Gudovic Radmila , Mijatov-Ukropina Ljilja , Stojsic-Dzunja Ljubica , Maric Dusica , Babovic Sinisa , Krstonosic Biljana
Published: June 2008

Introduction. The aim of this study was to identify the critical periods in the development of the human dentate nucleus in fetuses of different gestational ages and in one newborn brain. Material and Methods. The fetal brains were fixed in alcohol-formalin-acetic acid, embedded in paraffin, cut into 30m sections, and stained with cresyl violet. The sections were examined by light microscopy. In order to identify vulnerable periods, histological and stereological analyses were done. Formation of the dentate nucleus. The first appearance of the dentate nucleus was noticed in fetus of 12.5 weeks of gestation (wg), and its cells corresponded to the first and second stage of maturation. Formation of the dorsomedial lamina begins at the end of the 13th wg, and it starts to fold at 19.5 wg. At this time, cells correspond to the third stage of maturation, and formation of the ventromedial lamina begins. The first folds of the ventromedial lamina are noticed at 23.5 wg. Fourth stage maturity cells are noticed at 23.5 wg. remaining conspicuous up to birth. The numerical density of the nerve cell nuclei shows a constant decrease. Conclusion. Based on our results, we can conclude that during development of the dentate nucleus, there are two vulnerable periods. The first one corresponds to the fourth month of intrauterine life, and the second to the intensive growth of the dorsomedial and ventrolateral lamina (20.0 - 24.5 wg).
Laboratory medicine today
Authors: Dujmovic Ferenc , Djeric Mirjana , Stosic Zoran
Published: June 2008

Introduction. Laboratory medicine today is a well-established branch of medicine which has an important role in improving of diagnostics, research and all forms and levels of education at the Faculty of Medicine in Novi Sad. The value of laboratory medicine is particularly important in team work within the Clinical Center of Novi Sad, nowadays - Clinical Center of Vojvodina. Institute of Laboratory Medicine of the Clinical Center of Vojvodina. The institute of Laboratory Medicine of the Clinical Center of Vojvodina is a contemporary organized, integrated system of laboratories, employing highly educated professional staff, and with the exception of the nuclear-medicine laboratory, has all necessary equipment for providing the highest level of laboratory diagnostics. The Institute is a part of the international RIQAS (Randox International Quality Assessment Scheme) quality control and in the process of getting and accreditation. Today, the Institute offers a variety of over 400 different laboratory analyses and functional tests. The capacity of laboratory services is being significantly increased and is permanently growing. In addition, it is necessary to establish a PCR laboratory and a Department of Microbiology. Contemporary trends in organization of laboratory services. The Institute of Laboratory Medicine is also engaged in research, publishing and projects. It is a teaching hospital for the Department of Pathophysiology. This kind of approach to education of medical students ensures that pathophysiology provides a solid foundation for clinical studies at the Faculty of Medicine in Novi Sad.
Morphological parameters of prostatic adenocarcinoma
Authors: Trivunic Sandra , Budakov Pavle , Vuckovic Nada , Zivojinov Mirjana
Published: June 2008

Introduction. Prostatic carcinoma is one of the most common malignancies in men and the second most common cause of cancer-related deaths, after lung cancer. However, many of these tumors are latent, of no biologic significance, and are recognized only as an incidental finding at autopsy. Prostatic carcinoma: histologic features. Prostatic carcinomas have multiple histologic patterns, which can easily be confused with benign lesions. The following histologic changes are associated with prostatic carcinomas: prostatic acini are close to one another and present with linear infiltrates in the fibromuscular tissue; cells lining the acini often consist of a single layer, and the basal cell layer is absent; prominent large eosinophilic nucleoli are usually present in malignant cells; nuclear hyperchromatism is rare and it depends on the quality of the tissue fixation; perineural invasion is often observed. Today, the Gleason grading system is the most commonly used (Gleason score). Immunohistochemistry is widely used in pathology and clinical diagnosis of prostatic carcinoma, metastases of prostatic origin in staging malignant tumors and in the prognosis.