bluuebunny/crossref_metadata_2025 huggingface dataset:
Manifestation of phenotypic dominance in F1 and the degree of transgression in F2 by elements of productivity of the main ear of soft winter wheat
Authors: Humeniuk O. , Kyrylenko V. , Sabadyn V. , Dubovyk N.
Published: September 2023

The results of the study of phenotypic dominance in F 1 and the degree of transgression in F2 by elements of productivity: the number and mass of grains and the length of the main ear of soft winter wheat are presented. The selection of parental components for crossing was formed according to the scheme of 7x7 diallel crossings: by productivity (Podolianka, MIP Kniazhna, MIP Yuvileina, MIP Dovira), nutritional direction (Chornobrova, Biliava, Sofivka). In F 1, according to the length of the main spike, heterosis or overdominance was determined in 25 crossing combinations (61.0 %). The highest manifestation of heterosis was found in reciprocal crossings: MIP Kniazhna ↔ Biliava, MIP Yuvileina ↔ Sofivka, MIP Kniazhna ↔ MIP Yuvileina, etc. According to the number of grains in the main spike, heterosis was found in 26 crossing combinations (63.5 %) of MIP Kniazhna ↔ Chornobrova, Chornobrova ↔ MIP Yuvileina, MIP Dovira ↔ MIP Yuvileina, etc. By the mass of grains from the main ear, overdominance was found in 29 crossing combinations (72.5 %) of MIP Kniazhna ↔ Chornobrova, MIP Yuvileina ↔ Biliava, Sofivka ↔ Chornobrova, etc. These groups have the highest value for selection practice. In F 2, most populations signifcantly exceeded parental components, which indicates a signifcant formative process and the possibility of selection. According to the length of the main ear, positive transgression was found in 96.7 % of the hybrid populations. According to the number of grains from the main ear, the degree of positive transgression was determined in 32 hybrid populations (80.9 %). According to the mass of grains from the main ear - in 40 studied populations (97.5 %). A high degree of transgression was characterized by hybrid populations where varieties were used as the maternal form: MIP Kniazhna, MIP Yuvileina, MIP Dovira, Sofivka, Chornobrova, etc.
Key words: winter wheat, F1 hybrids, productivity elements, phenotypic dominance, F2 populations, transgression.
Characteristics of ecological risks in ecosystems and their manifestation possibility assessment
Authors: Khyzhnyak S. , Koversun I. , Dovbysh O. , Voytsitsky V.
Published: September 2023

Environment pollution with ecotoxicants of natural and anthropogenic origin creates risks of negative impact on the state of the environment, the biota of ecosystems, including humans. The study defnes the main sources of ecotoxic pollutants and analyzes the risks that may arise. Based on the ambiguity of the "risk" term defnition, the interpretation of the term is discussed. The approach to defning risk as the probability of certain adverse events or processes taking into account possible consequences is noted. The relationship between environmental risks and the reliability of ecosystems is indicated. Environmental risk assessment is a complex process that involves identifying the likelihood of reversible or irreversible changes in the structure and functions of ecosystems in response to anthropogenic or natural impacts. The quantitative measure of risk as a threat of danger is the product of the probability of an adverse outcome of the event and the expected loss as a result of the event. Considerable attention is paid to the assessment of possible ecological risks for agroecosystems, which are associated with environmental degradation, the impact on human health, and the possible ingress of toxic substances into food products and pet fodder. Ecological risks in agroecosystems arise, in particular, as a result of the use of plant protection products (pesticides), excessive use of agrochemicals. However, the process of risk management in agroecosystems involves the selection and use of risk reduction methods, in particular, the use of measures to regulate the impact of ecotoxicants by limiting, banning the most toxic and preventing pollution. The study of environmental risk is necessary to understand the impact of harmful factors on environmental components. It is emphasized that risk management in agriculture should be aimed at preserving the agroecosystem optimal state and protecting human health.
Key words: environmental risk, environment, ecotoxicants, rating, danger, agroecosystem, pesticides.
Institute of bioenergy crops and sugar beet NAAS: bioenergy crops gene bank formation and use in breeding
Authors: Orlov S. , Kornieieva M. , Kulyk O. , Brovko S.
Published: September 2023

For a long time, researchers have been studying and enriching the gene bank with new sources of valuable economic and biological traits and using them in the research and breeding process. The studied species include sugar beet, fodder beet, table beet, wild beet, miscanthus, switchgrass, energy willow and poplar. These species are used for sugar and biofuel production. The aim of the research was to study the morphological, biological and economic characteristics of the breeding genotypes of beet and bioenergy crops and select the valuable ones for breeding and ensuring increased energy output and high productivity, to replenish and preserve the collections of plant genetic resources to ensure the research and breeding programs. Of special interest are the genotypes with the sterility maintaining ability, fertility and other valuable traits that can be used in the development of bioenergy CMS hybrids. Basic collections of beet (sugar beet, fodder beet and wild beet species), bioenergy crops (switchgrass, miscanthus, willow, poplar) have been composed. The selected genotypes included switchgrass ‘Ya/Kei’ with an increased yield of solid biofuel up to 2.4 t/ha compared to the standard variety ‘Carthage’; willow 'Tordis' with a high biomass yield of 30.62 t/ha and calorifc value up to 18.4 MJ/kg; miscanthus M. sinensis ‘Grosse Fontane’ with solid biofuel yield of 176 % and energy of 299.1 GJ/ha; sugar beet with increased indicators of economic and valuable traits ‘ISV–STs200190’ (root yield 109.2 %, sugar content 101.2 %, sugar yield 110.5 %, sugar output 117.8 %), ‘ISV-STs200181 (root yield 110.1 %, sugar content 102.0 %, sugar yield 112.3 %, sugar output 118.0 %). Researchers of the institute conduct research on the development of new sources and donors of bio feedstock materials, the introduction of new species, improvement of varietal composition and methods of reproduction and increase the efciency of bio feedstock production with high calorifc value in order to reduce the country's energy dependence and improve the ecological state of the environment.
Key words: gene pool, collections, samples, sources, hybrids, varieties, lines.
PENERAPAN MODEL PROBLEM BASED LEARNING BERBANTUAN ALAT PERAGA SEDERHANA UNTUK MENINGKATKAN HASIL BELAJAR KELAS VIII SMPN 04 KOTA BENGKULU
Authors: Hidayati Latifa Nurliyan , Yensy Nurul Astuty
Published: September 2023

Penelitian ini bertujuan untuk meningkatkan hasil belajar dan aktivitas belajar peserta didik Kelas VIII SMP Negeri 04 Kota Bengkulu dengan menerapkan model Problem Based Learning berbantuan alat peraga sederhana dalam mengelola kelas. Jenis penelitian ini adalah penelitian tindakan kelas (PTK) dengan 4 tahapan yaitu perencanaan, pelaksanaan tindakan, observasi dan refleksi. Subjek penelitian ini adalah peserta didik Kelas VIII 4 SMP Negeri 04 Kota Bengkulu semester ganjil tahun ajaran 2022/2023 yang berjumlah 34 orang peserta didik. Pengumpulan data penelitian dilakukan dengan menggunakan lembar observasi aktivitas belajar peserta didik dan lembar tes akhir siklus. Peningkatan aktivitas belajar peserta didik dapat dilihat dari hasil observasi aktivitas belajar peserta didik yaitu pada siklus 1 aktivitas peserta didik berada pada kriteria cukup aktif, sedangkan pada siklus 2 aktivitas peserta didik menjadi aktif. Peningkatan hasil belajar peserta didik dapat dilihat dari skor rata-rata hasil belajar peserta didik dari siklus I sampai siklus II yaitu:  72,9; 80,0.
The Switching Mechanisms of Dihydroazulene/ Vinylheptafulvene Photo-/Thermoswitches
Authors:   Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen Ø, Denmark, Gertsen Anders S. , Mikkelsen Kurt V. ,   Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen Ø, Denmark
Published: September 2023

We have investigated the switching mechanisms of photochromic systems that are relevant for harvesting, storing, and releasing solar energy. We consider storage of solar energy in the form of energetic chemical bonds via light-induced isomerization reactions. The approach concerns photoswitches that upon irradiation isomerize to high-energy metastable isomers and through the corresponding backreaction will release the stored energy. Thereby, we have considered molecular closed-cycle energy storage systems that do not involve the release of CO2 . We present potential energy surfaces for ground states and excited states and discuss the roles that these states play when harvesting, storing, and releasing solar energy. Furthermore, we have illustrated new approaches for obtaining synergy between theoretical modeling and future experimental work.
An Automatic Fast High Resolution HDL Subfractioning Assay Using Microfluidic Electrophoretic System
Authors:   Gaozhou People Hospital, Guangdong, China, Lin Qiuwei , Deng Bo ,   Gaozhou People Hospital, Guangdong, China, Zeng Hanbin ,   Guangdong Ardent Biomedical Co. LTD, China, Zhang Can ,   Gaozhou People Hospital, Guangdong, China, Lin Chunfen ,   Guangdong Ardent Biomedical Co. LTD, China, Mo Ni ,   Gaozhou People Hospital, Guangdong, China, Zhu Xiulong ,   Gaozhou People Hospital, Guangdong, China, Deng David ,   Guangdong Ardent Biomedical Co. LTD, China
Published: September 2023

Objectives: Studies in recent decade demonstrated the importance of HDL subfractionation in precision medicine. It is notable that not all HDL subfractions are “good” lipoprotein. The lack of a reliable technique that can precisely identify each particle of HDL subfractions remains the major obstacle in clinical application. Based on the principle of microfluidic electrophoresis, we have developed an automatic system (MFE) that meets the need of clinical applications. The objectives of this paper is to test the system for its application, precision, and also the normal range in healthy population using one of major subfraction, HDL-2b, as a target. Methods: The MFE was compared with traditional non-denaturing polyacrylamide gradient gel electrophoresis (GGE). Intra-chip, inter-chip, inter-instrument, inter-lab, and inter-operator precisions were all tested by using the sample in various conditions. An instruction was provided with the instrument and HDL subfractioning kit. Data were analyzed with SPSS for correlation of variation, regressions, etc. Results: The new system exhibited a linear correlation with the traditional GGE system. The intra-chip %CVs, inter-chip %CVs, inter-instrument %CVs of HDL-2b were less than 10%, which was the set acceptance criteria. With 243 samples from healthy individuals demonstrated that the mean value of HDL-2b% is about 25% in both male and female. Conclusions: Our data demonstrated that the MFE system is a fast, automatic, and reliable system that can obtain the percentage of HDL-2b with high precision
The Principle of Commodity Utility and Its Equations
Authors:   Anhui Grain Engineering Vocational College, Anhui, China, Cheng Jinjun , Cheng Dian ,   China Cinda Asset Management Co., Ltd., China
Published: September 2023

"Das Kapital" points out that commodities have two attributes of value and use value at the same time, and describe both qualitatively rather than quantitatively. The utility of commodities fundamentally comes from the functional attributes of natural things, and must rely on the use value of commodities to play its role. People's behavior of buying goods is based on the evaluation of the utility of goods when making the decision to buy goods. The process of evaluating the utility of a commodity when making a decision to buy it is essentially a process of comparing their labor efforts and obtaining happiness in their heart. The equation of utility is: U= (1 / P) (Pm / W); the unit of measurement is named "Om" (symbol in Sanskrit ॐ), is the reciprocal of the monetary unit "Tarrant" (symbol Ω). Commodity utility theory of value and commodity labor theory of value are unified through the utility equation.
Determination of the Relationship between Attitude towards Food Supplements and Physical Health Perception
Authors: Tabuk Mustafa Ertan
Published: September 2023

In this age when the importance of human and public health is increasing, inactivity, which is seen as one of the most important problems, and the increase in obesity rates threaten public health. Therefore, the importance of physical activity and nutrition is increasing and new researches are being conducted in this field. The aim of this study is to analyse the relationship between physical health perception and attitude towards the usage of food supplements. Relational survey method was used in this study designed to determine this relationship between variables. 287 academic staff (85= female, 202= male) participated in the study. The physical health statements of the PROMIS global health scale and the scale adapted to the use of food supplements after a literature review were used. As a result, significant relationships were determined between attitude towards the use of food supplements and physical health perception. It was determined that academicians generally considered themselves healthy and were open-minded about the use of food supplements (F=8.150 p=0.000), physical health perception differed according to their field of study (F=4.912; p=0.002), and those who exercised regularly had a higher physical health perception (F=8,339; p>0.05). The study revealed important results in terms of guiding individual, public health and government policies. It is recommended for new researchers to examine the concepts related to rest, sleep behaviours and nutritional knowledge.
Immature lung and acute lung injury.
Authors: Zoban P , Černý M
Published: September 2023

Acute lung injury occurs mostly in the very low birth weight and extremely low birth weight infants. The pathological process leading to acute lung injury includes immature and/or diseased lung that experienced oxidative stress, inflammation and mechanical insult with the bronchial, alveolar and capillary injuries and cell death. It may be the first step to the subsequent development of chronic lung disease of prematurity or bronchopulmonary dysplasia. The mechanisms of lung injury are extensively investigated in the experimental models and clinical studies, mostly performed on the adult patients. At present, the explanations of the mechanism(s) leading to lung tissue injury in tiny premature babies are just derived from these studies. Acute lung injury seems to be rather a syndrome than a well-defined nosological unit and is of multifactorial etiology. The purpose of this review is to discuss the main factors contributing to the development of acute lung injury in the very low or extremely low birth weight infants – lung immaturity, mechanical injury, oxidative stress and inflammation. Nevertheless, numerous other factors may influence the status of immature lung after delivery.
Inhibitory effects of Bcl-2 on mitochondrial respiration.
Authors: Vrbacký M , Krijt J , Drahota Z , Mělková Z
Published: September 2023

In contrast to the well-established anti-apoptotic effect of Bcl-2 protein, we have recently demonstrated that Bcl-2 overexpression by vaccinia virus causes apoptosis in BSC-40 cells, while it prevents apoptosis in HeLa G cells. Given the key role of mitochondria in the process of apoptosis, we focused on effects of Bcl-2 expression on mitochondrial energetics of these two cell lines. In this study we present data indicating that BSC-40 cells derive their ATP mainly from oxidative phosphorylation whereas HeLa G cells from glycolysis. More importantly, we show that in both cell lines, Bcl-2 inhibits mitochondrial respiration and causes a decrease of the ATP/ADP ratio. However, it appears that BSC-40 cells cannot sustain this decrease and die, while HeLa G cells survive, being adapted to the low ratio of ATP/ADP maintained by glycolysis. Based on this observation, we propose that the outcome of Bcl-2 expression is determined by the type of cellular ATP synthesis, namely that Bcl-2 causes apoptosis in cells relying on oxidative phosphorylation.
UNSAFE BEHAVIOR ANALYSIS: A DEEP QUESTIONNAIRE SURVEY IN CONSTRUCTION SITES
Authors: M B Meharnisa , M S Geethu ,   SNIT
Published: September 2023

The numerous mishaps and injuries that take place on building sites in Kerala, India, highlight the need for in-depth research to prevent similar incidents in the future. An exhaustive questionnaire survey is a method for obtaining information on accidents and injuries that provides comprehensive data to identify root causes and develop prevention strategies. With this approach, injured or accident-plagued employees are required to complete a lengthy questionnaire that asks questions about the incident's circumstances, timing, tools, directives, and safety procedures. A detailed questionnaire survey is an efficient and affordable technique to gather information that may be tailored to the unique needs of Kerala construction sites. A comprehensive questionnaire survey can be a useful tool for enhancing safety on building sites in Kerala, despite its drawbacks such as response rate and questionnaire design. The data collected can help identify areas for development and lower the frequency of accidents and injuries on construction sites.
ERROR CORRECTION USING QUANTUM COMPUTING
Authors: khan Md Khalik , jain Dr Sapna ,   SEST, Jamia Hamdard
Published: September 2023

Quantum Error Correction (QEC) is an important technique for protecting quantum information against decoherence and errors. This involves the design and implementation of algorithms and techniques to minimize error rates and increase the stability of quantum circuits. One of the key parameters in QEC is the distance of the error- correcting code, which determines the number of errors that can be corrected. Another important parameter is the error probability, which quantifies the likelihood of errors occurring in the quantum system. In this context, the goal of a simulation sweep like the one performed in the code is to study the performance of the QEC code for different values of the distance and error probability, and to optimize the code for maximum accuracy. By varying these parameters and observing the performance of the code, researchers can gain insights into how to design better codes and improve the reliability of quantum computing systems. We also discuss the challenges that need to be addressed for quantum computing to realize its potential in solving practical Error-correction problems.
INDUSTRY 5.0: SUSTAINABLE DEVELOPMENT IN BANGLADESH
Authors: Rana Md Shohel , Das Saikat , Shikder Sayem , Ahmad Shakil ,   Chandigarh University
Published: September 2023

After liberation war, Bangladesh emerged as one of the world’s largest industrial powers, opening its political and economic influence. The 5th Industrial Revolution is the next industrial revolution, and its goal is to harness human experts in cooperation with smart machines and robots, to obtain manufacturing with higher resource efficiency and easier use compared to the 4th Industrial Revolution. It is expected that the focus will be on the human side, sustainability, and the environment. This paper looks at the industrial policy in Bangladesh, emphasizing the continuous coordinating role of the government in various fields and that Bangladesh will be one of the world’s most trends towards investment and a suitable environment to keep pace with the latest industrial transformations. We aim to present a vision of the 5th industry, its applications, the techniques used, and the importance of developing countries in this field. action.
Tyrosine-Mutant AAV8 Vector Mediated Efficient and Safe Gene Transfer of Pigment Epithelium-Derived Factor to Mouse Lungs
Authors: Ferreira Débora P. , Martini Sabrina V. , Oliveira Helena A. , Silva Adriana L. , Shenoy Siddharth , Chen Daiqin , Simon Valentina , Han Eric , West Natalie E. , Suk Jung Soo , Rocco Patricia R.M. , Petrs-Silva Hilda , Morales Marcelo M. , Cruz Fernanda F.
Published: September 2023

Background/Aims: Recombinant adeno-associated viruses (rAAV) are an important tool for lung targeted gene therapy. Substitution of tyrosine with phenylalanine residues (Y-F) in the capsid have been shown to protect the AAV vector from ubiquitin/proteasome degradation, increasing transduction efficiency. We tested the mutant Y733F-AAV8 vector for mucus diffusion, as well as the safety and efficacy of pigment epithelium-derived factor (PEDF) gene transfer to the lung. Methods: For this purpose, Y733F-AAV8-PEDF (1010 viral genome) was administered intratracheally to C57BL/6 mice. Lung mechanics, morphometry, and inflammation were evaluated 7, 14, 21, and 28 days after injection. Results: The tyrosine-mutant AAV8 vector was efficient at penetrating mucus in ex vivo assays and at transferring the gene to lung cells after in vivo instillation. Increased levels of transgene mRNA were observed 28 days after vector administration. Overexpression of PEDF did not affect in vivo lung parameters. Conclusion: These findings provide a basis for further development of Y733F-AAV8-based gene therapies for safe and effective delivery of PEDF, which has anti-angiogenic, anti-inflammatory and anti-fibrotic activities and might be a promising therapy for lung inflammatory disorders.
TÜRKİYE’DEKİ ÜNİVERSİTELERDE SPOR BİLİMLERİ FAKÜLTELERİ, BEDEN EĞİTİMİ VE SPOR YÜKSEKOKULLARI İLE BEDEN EĞİTİMİ VE SPOR BÖLÜMLERİNİN WEB SAYFALARINDAKİ MİSYON VE VİZYON İFADELERİNİN TEMATİK OLARAK ANALİZİ
Authors: KOSE Hande , ÖZDİLEK Çetin
Published: September 2023

Bu araştırmada Türkiye’deki devlet ve vakıf üniversiteleri bünyesinde yer alan Spor Bilimleri Fakülteleri, Beden Eğitimi ve Spor Yüksekokulları ile Beden Eğitimi ve Spor Bölümlerinin misyon ve vizyon ifadelerinin tematik açıdan incelenmesi ve analiz edilmesi amaçlanmaktadır. Araştırmada nitel araştırma yöntemlerinden “Olgu Bilim/Fenomoloji” deseni kullanılmış olup veriler “Doküman İnceleme” tekniği ile toplanmıştır. Elde edilen verilerin çözümlenmesinde “İçerik Analizi” yöntemi kullanılmıştır. Araştırmanın evrenini 85 devlet ve 15 vakıf üniversitesinin bünyesinde bulunan Spor Bilimleri Fakülteleri, Beden Eğitimi ve Spor Yüksekokulları ile Beden Eğitimi ve Spor Bölümlerinin internet sayfalarında yer alan misyon ve vizyon ifadeleri oluşturmaktadır. Vakıf üniversitelerin 9’unda misyon ve vizyon ifadeleri yerine mütevelli heyetinin düşünce ve değerler ifadelerine yer verilmiş olup bu açıklamalar çalışmaya dahil edilmemiştir. Araştırma sonucunda misyon ve vizyon ifadeleri üç ana kategori altında toplanmıştır. Misyon ifadelerinde sıklıkla görülen ana kategori “Nitelikli Birey Yetiştirme Fonksiyonu” fonksiyonu olup vizyon ifadelerinde ise en sık görülen ana kategori “Eğitim Öğretim Fonksiyonu” olduğu tespit edilmiştir.
ASESMEN PEMBELAJARAN SEKOLAH DASAR DALAM KURIKULUM MERDEKA
Authors: Mujiburrahman Mujiburrahman , Kartiani Baiq Sarlita , Parhanuddin Lalu
Published: September 2023

Assessment or assessment is a series of activities carried out systematically continuously which is used as a collection of information about the learning process and outcomes of students to obtain decisions based on certain criteria and considerations. The purpose of implementing learning assessment is as an effort to collect and process information to find out the learning needs, developmental achievements and learning outcomes of students.  The method used is historical research or documentary study by collecting various information through news, through official government documents, and so on. The results of this study are known that the assessment used in elementary schools refers to Permendikbud No. 21 of 2022, namely formative assessment and summative assessment.  A formative session is carried out at the beginning of the learning process while a summative assessment is carried out at the end of the learning activity. The independent curriculum has a big vision to create a happy and quality learning and learning atmosphere. With the aim of creating fun education, exploring the potential of teachers and students, developing the potential of students, catching up and improving the quality of learning independently.
ЛЕЧЕБНЫЕ ПРАКТИКИ РУССКОГО СЕВЕРА: ТРАВОЛЕЧЕНИЕ В КЕНОЗЕРЬЕ
Authors: Керимова М.М.
Published: September 2023

Кенозерский национальный парк – уникальная, особо охраняемая территория, включенная во Всемирную сеть биосферных заповедников ЮНЕСКО. Этим во многом объясняется сохранность широкого разнообразия произрастающих здесь растений, многие из которых внесены в Красную книгу. Распространенные в Кенозерье народные целительские практики весьма продуктивно способствуют поддержанию здоровья местного населения. Проверенные многими годами использования, они остаются в традиционной практике местных травниц, широко применяются сельскими жителями, а также вводятся в промышленное производство: их продают в виде сборов в киосках туристических объектов Кенозерья и в виде отдельных фитопрепаратов в аптеках Архангельской области. Удаленность от больших городов и отсутствие в прошлом и настоящем надлежащего медицинского обслуживания способствовало тому, что жители этого региона активно использовали целебные свойства лекарственных растений. В статье рассматривается история изучения и использования флоры Кенозерья. На основании полевых материалов, собранных у местных травниц летом 2021 г. в д. Морщихинская на Лекшмозере, анализируются фитотерапевтические свойства некоторых лекарственных растений.
A porous form Coomassie brilliant blue G250-isorhamnetin fluorescent composite coated with acrylic resin for tumor cell imaging
Authors: Hu Jiangpeng , Teng Bo , Xu Zhipeng , Wan Yuanye , Jin Guofan
Published: September 2023

Four distinct fluorescence complexes, the fluorescent complex-1 (FC-1), fluorescent complex-2 (FC-2), fluorescent complex third (FC-3) and fluorescent complex fourth (FC-4), were created using isorhamnetin and Coomassie brilliant blue G250 as raw materials. The issue of isorhamnetin’s low solubility has been resolved, and isorhamnetin-coomassie brilliant blue G250 now has better biocompatibility. Four different forms of fluorescence compounds’ ultraviolet absorption spectra were identified. It was discovered that FC-2, FC-3, and FC-4, respectively, had double peaks at 483–620 nm. FC-4 had the highest ultraviolet absorption intensity, whereas FC-1 exhibited the most consistent and longest wavelength of ultraviolet absorption. Transmission electron microscopy revealed that the acrylic resin evenly disseminated the Coomassie brilliant blue G250-isorhamnetin complex in an amorphous flocculent form. Human prostate cancer cells (PC3) and human cervical cancer cells (HeLa) were investigated in the (Cell Counting Kit-8) CCK8 experiment under 10 different concentration circumstances, and the proliferation impact was 64.30% and 68.06%, respectively. Shown the complex’s strong anti-tumor properties and minimal cytotoxicity. Through in vitro imaging of tumor cells, it was found that FC-1’s fluorescent complex has high selectivity and can accurately infiltrate tumor cells, proving that it is biocompatible. The design not only addresses the issue of isorhamnein-Coomassie Bright Blue G250’s bioavailability, but it also has an effective visual fluorescence targeting effect.
The gut microbiota as a potential biomarker for methamphetamine use disorder: evidence from two independent datasets
Authors: Liu Linzi , Deng Zijing , Liu Wen , Liu Ruina , Ma Tao , Zhou Yifang , Wang Enhui , Tang Yanqing
Published: September 2023

Background

Methamphetamine use disorder (MUD) poses a considerable public health threat, and its identification remains challenging due to the subjective nature of the current diagnostic system that relies on self-reported symptoms. Recent studies have suggested that MUD patients may have gut dysbiosis and that gut microbes may be involved in the pathological process of MUD. We aimed to examine gut dysbiosis among MUD patients and generate a machine-learning model utilizing gut microbiota features to facilitate the identification of MUD patients.

Method

Fecal samples from 78 MUD patients and 50 sex- and age-matched healthy controls (HCs) were analyzed by 16S rDNA sequencing to identify gut microbial characteristics that could help differentiate MUD patients from HCs. Based on these microbial features, we developed a machine learning model to help identify MUD patients. We also used public data to verify the model; these data were downloaded from a published study conducted in Wuhan, China (with 16 MUD patients and 14 HCs). Furthermore, we explored the gut microbial features of MUD patients within the first three months of withdrawal to identify the withdrawal period of MUD patients based on microbial features.

Results

MUD patients exhibited significant gut dysbiosis, including decreased richness and evenness and changes in the abundance of certain microbes, such as Proteobacteria and Firmicutes. Based on the gut microbiota features of MUD patients, we developed a machine learning model that demonstrated exceptional performance with an AUROC of 0.906 for identifying MUD patients. Additionally, when tested using an external and cross-regional dataset, the model achieved an AUROC of 0.830. Moreover, MUD patients within the first three months of withdrawal exhibited specific gut microbiota features, such as the significant enrichment of Actinobacteria. The machine learning model had an AUROC of 0.930 for identifying the withdrawal period of MUD patients.

Conclusion

In conclusion, the gut microbiota is a promising biomarker for identifying MUD and thus represents a potential approach to improving the identification of MUD patients. Future longitudinal studies are needed to validate these findings.
Application of machine learning and artificial intelligence in the diagnosis and classification of polycystic ovarian syndrome: a systematic review
Authors: Barrera Francisco J. , Brown Ethan D.L. , Rojo Amanda , Obeso Javier , Plata Hiram , Lincango Eddy P. , Terry Nancy , Rodríguez-Gutiérrez René , Hall Janet E. , Shekhar Skand
Published: September 2023

Introduction

Polycystic Ovarian Syndrome (PCOS) is the most common endocrinopathy in women of reproductive age and remains widely underdiagnosed leading to significant morbidity. Artificial intelligence (AI) and machine learning (ML) hold promise in improving diagnostics. Thus, we performed a systematic review of literature to identify the utility of AI/ML in the diagnosis or classification of PCOS.

Methods

We applied a search strategy using the following databases MEDLINE, Embase, the Cochrane Central Register of Controlled Trials, the Web of Science, and the IEEE Xplore Digital Library using relevant keywords. Eligible studies were identified, and results were extracted for their synthesis from inception until January 1, 2022.

Results

135 studies were screened and ultimately, 31 studies were included in this study. Data sources used by the AI/ML interventions included clinical data, electronic health records, and genetic and proteomic data. Ten studies (32%) employed standardized criteria (NIH, Rotterdam, or Revised International PCOS classification), while 17 (55%) used clinical information with/without imaging. The most common AI techniques employed were support vector machine (42% studies), K-nearest neighbor (26%), and regression models (23%) were the commonest AI/ML. Receiver operating curves (ROC) were employed to compare AI/ML with clinical diagnosis. Area under the ROC ranged from 73% to 100% (n=7 studies), diagnostic accuracy from 89% to 100% (n=4 studies), sensitivity from 41% to 100% (n=10 studies), specificity from 75% to 100% (n=10 studies), positive predictive value (PPV) from 68% to 95% (n=4 studies), and negative predictive value (NPV) from 94% to 99% (n=2 studies).

Conclusion

Artificial intelligence and machine learning provide a high diagnostic and classification performance in detecting PCOS, thereby providing an avenue for early diagnosis of this disorder. However, AI-based studies should use standardized PCOS diagnostic criteria to enhance the clinical applicability of AI/ML in PCOS and improve adherence to methodological and reporting guidelines for maximum diagnostic utility.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42022295287.
Association between estradiol levels in early pregnancy and risk of preeclampsia after frozen embryo transfer
Authors: Hsieh Yun-Chiao , Kao Tzu-Ching , Yang Ih-Jane , Yang Po-Kai , Chao Kuang-Han , Chen Mei-Jou , Yang Jehn-Hsiahn , Chen Shee-Uan
Published: September 2023

Introduction

The failure of remodeling the spiral arteries is associated with the pathogenesis of preeclampsia. Estradiol (E2) plays a crucial role in placentation and may be involved in the development of preeclampsia. However, there is a lack of data in this area. This study aims to assess the association between serum estradiol levels in early pregnancy and the risk of preeclampsia.

Methods

We conducted a retrospective cohort study on patients who conceived after frozen embryo transfer (FET) using data from a database at a university-affiliated in vitro fertilization center. The study period spanned from January 1, 2010, to December 31, 2020. Multivariable logistic regression analyses were performed to determine the adjusted effect of E2 levels on the risk of preeclampsia. We compared the odds ratios of preeclampsia across quartiles of E2 levels and assessed their significance.

Results

Serum E2 levels at the fifth gestational week were significantly different between women with and without preeclampsia after FET programmed cycles (607.5 ± 245.4 vs. 545.6 ± 294.4 pg/ml, p=0.009). A multivariable logistic regression model demonstrated that E2 levels in early pregnancy were independent risk factors for preeclampsia. We observed an increased odds ratio of preeclampsia with increasing quartiles of estradiol levels after adjusting for potential confounders in FET programmed cycles. When comparing quartiles 3 and 4 (E2 > 493 pg/ml at the fifth gestational week) to quartiles 1 and 2, the odds ratios of preeclampsia were significantly higher.

Conclusion

We found that serum E2 levels in early pregnancy may impact the risk of preeclampsia, particularly following FET programmed cycles. The association between E2 levels in early pregnancy and preeclampsia deserves further investigation.
Unkeito promotes follicle development by restoring reduced follicle-stimulating hormone responsiveness in rats with polycystic ovary syndrome
Authors: Yoshita Sayako , Osuka Satoko , Shimizu Tomofumi , Fujitsuka Naoki , Matsumoto Chinami , Bayasula  , Miyake Natsuki , Muraoka Ayako , Nakanishi Natsuki , Nakamura Tomoko , Goto Maki , Kajiyama Hiroaki
Published: September 2023

Background

Polycystic ovary syndrome (PCOS) is a common disorder resulting in irregular menstruation and infertility due to improper follicular development and ovulation. PCOS pathogenesis is mediated by downregulated follicle-stimulating hormone receptor (FSHR) expression in granulosa cells (GCs); however, the underlying mechanism remains elusive. Unkeito (UKT) is a traditional Japanese medicine used to treat irregular menstruation in patients with PCOS. In this study, we aimed to confirm the effectiveness of UKT in PCOS by focusing on follicle-stimulating hormone (FSH) responsiveness.

Methods

A rat model of PCOS was generated by prenatal treatment with 5α-dihydrotestosterone. Female offspring (3-week-old) rats were fed a UKT mixed diet or a normal diet daily. To compare the PCOS phenotype in rats, the estrous cycle, hormone profiles, and ovarian morphology were evaluated. To further examine the role of FSH, molecular, genetic, and immunohistological analyses were performed using ovarian tissues and primary cultured GCs from normal and PCOS model rats.

Results

UKT increased the number of antral and preovulatory follicles and restored the irregular estrous cycle in PCOS rats. The gene expression levels of FSHR and bone morphogenetic protein (BMP)-2 and BMP-6 were significantly decreased in the ovarian GCs of PCOS rats compared to those in normal rats. UKT treatment increased FSHR staining in the small antral follicles and upregulated Fshr and Bmps expression in the ovary and GCs of PCOS rats. There was no change in serum gonadotropin levels. In primary cultured GCs stimulated by FSH, UKT enhanced estradiol production, accompanied by increased intracellular cyclic adenosine monophosphate levels, and upregulated the expression of genes encoding the enzymes involved in local estradiol synthesis, namely Cyp19a1 and Hsd17b. Furthermore, UKT elevated the expression of Star and Cyp11a1, involved in progesterone production in cultured GCs in the presence of FSH.

Conclusions

UKT stimulates ovarian follicle development by potentiating FSH responsiveness by upregulating BMP-2 and BMP-6 expression, resulting in the recovery of estrous cycle abnormalities in PCOS rats. Restoring the FSHR dysfunction in the small antral follicles may alleviate the PCOS phenotype.
Integrated energy system optimization and scheduling method considering the source and load coordinated scheduling of thermal-storage electric boilers and electric vehicles
Authors: Yang Yulong , Wu Xinge , Zhang Ziye , Jin Rong , Zhang Chong , Li Songyuan , Yi Pengyu
Published: September 2023

The northern regions of China face the challenges of the mismatch of the power supply and demand, as well as serious wind curtailment issues, caused mainly by the limitation of the “with heat to determine electricity” mode for combined heat and power generation during the winter season. To further absorb the surplus wind power and alleviate restrictions, a comprehensive energy system optimization method for parks based on coordinated scheduling between sources and loads is proposed in this paper. First, the implementation of a heat-storage electric boiler on the source side further achieves the decoupling of heat and power. Second, an optimized scheduling method for electric vehicles combining incentive scheduling and orderly scheduling is proposed on the load side, which helps flatten the load curve. Finally, a tiered carbon trading mechanism is introduced and a community integrated energy system (CIES) optimization scheduling model is established with the aim of minimizing the total cost of the CIES, and the problem is solved using the CPLEX commercial solver. The simulation results indicate that the overall system efficiency is significantly improved through the coordinated scheduling of power sources and loads. Specifically, the integration rate of wind power increases by 3.91% when compared to the sole consideration of the integrated demand response. Furthermore, the peak shaving and off-peak filling effect is considerably enhanced compared to the utilization of only thermal-storage electric boilers. Additionally, the implementation of coordinated scheduling leads to a reduction in the total system cost by 2764.32 yuan and a decrease in total carbon emissions by 3515.4 kg. These findings provide compelling evidence that the coordinated scheduling of power sources and loads surpasses the limitations of thermal power units, strengthens the demand response capability of electric vehicles, and enhances the economic benefits of the CIES.
Traceability analysis for low-voltage distribution network abnormal line loss using a data-driven power flow model
Authors: Sun Zhiqing , Xuan Yi , Huang Yi , Cao Zikai , Zhang Jiansong
Published: September 2023

The abnormal behavior of end-users is one of the main causes of abnormal line loss in distribution networks. The integration of a large amount of distributed renewable energy into a low-voltage distribution network (LVDN) complicates line loss analysis. Traceability analysis for abnormal line loss aims to identify the specific end-user responsible for the anomaly in line loss. This paper proposes, for LVDNs with incomplete topology and line parameters, a practical traceability analysis approach using a data-driven power flow model. A data-driven power flow model based on a neural network is first established to capture the power flow mapping relationship without topology and line parameter information. A backpropagation algorithm is then presented to correct the actual power consumption data according to the measured voltage data. By comparing actual power consumption data with measured power data, users with abnormal behavior can be accurately identified and tracked. Finally, the effectiveness of the proposed approach is verified by actual data.
Impact of fintech and environmental regulation on green innovation: inspiration from prefecture-level cities in China
Authors: Ni Lin , Yu Yalu , Wen Huwei
Published: September 2023

Environmental regulations may promote regional ecological evolution, but they also increase the need for financing green innovation activities. This study uses panel data from prefecture-level cities in China to examine the impact of fintech and environmental regulation on regional green innovation in the digital economy era. Empirical evidence shows that fintech significantly promotes regional green innovation, and fintech has a positive interaction effect with environmental regulation. While the evidence generally supports the role of environmental regulations in promoting green innovation, the evidence is insignificant in some models. The synergistic effect of fintech and environmental regulation on utility model green innovation is significant, but not on invention type green innovation. Climate policy, as a carbon regulatory policy, does not directly lead to green innovation, but it significantly collaborates with fintech to promote green innovation. The effects of fintech and environmental regulation on green innovation also have heterogeneity effects between resource-based and non-resource-based cities, and non-resource-based cities have a greater effect on achieving green innovation through fintech and environmental regulation. Our findings contribute to optimizing the coordination system between financial and environmental policies, thereby driving regional green innovation development with fintech in the digital age.
Exploiting the molecular subtypes and genetic landscape in pancreatic cancer: the quest to find effective drugs
Authors: Elebo Nnenna , Abdel-Shafy Ebtesam A. , Cacciatore Stefano , Nweke Ekene Emmanuel
Published: September 2023

Pancreatic Ductal Adenocarcinoma (PDAC) is a very lethal disease that typically presents at an advanced stage and is non-compliant with most treatments. Recent technologies have helped delineate associated molecular subtypes and genetic variations yielding important insights into the pathophysiology of this disease and having implications for the identification of new therapeutic targets. Drug repurposing has been evaluated as a new paradigm in oncology to accelerate the application of approved or failed target-specific molecules for the treatment of cancer patients. This review focuses on the impact of molecular subtypes on key genomic alterations in PDAC, and the progress made thus far. Importantly, these alterations are discussed in light of the potential role of drug repurposing in PDAC.
Correlation between pseudotyped virus and authentic virus neutralisation assays, a systematic review and meta-analysis of the literature
Authors: Cantoni Diego , Wilkie Craig , Bentley Emma M. , Mayora-Neto Martin , Wright Edward , Scott Simon , Ray Surajit , Castillo-Olivares Javier , Heeney Jonathan Luke , Mattiuzzo Giada , Temperton Nigel James
Published: September 2023

Background

The virus neutralization assay is a principal method to assess the efficacy of antibodies in blocking viral entry. Due to biosafety handling requirements of viruses classified as hazard group 3 or 4, pseudotyped viruses can be used as a safer alternative. However, it is often queried how well the results derived from pseudotyped viruses correlate with authentic virus. This systematic review and meta-analysis was designed to comprehensively evaluate the correlation between the two assays.

Methods

Using PubMed and Google Scholar, reports that incorporated neutralisation assays with both pseudotyped virus, authentic virus, and the application of a mathematical formula to assess the relationship between the results, were selected for review. Our searches identified 67 reports, of which 22 underwent a three-level meta-analysis.

Results

The three-level meta-analysis revealed a high level of correlation between pseudotyped viruses and authentic viruses when used in an neutralisation assay. Reports that were not included in the meta-analysis also showed a high degree of correlation, with the exception of lentiviral-based pseudotyped Ebola viruses.

Conclusion

Pseudotyped viruses identified in this report can be used as a surrogate for authentic virus, though care must be taken in considering which pseudotype core to use when generating new uncharacterised pseudotyped viruses.
Surface immunogenic protein from Streptococcus agalactiae and Fissurella latimarginata hemocyanin are TLR4 ligands and activate MyD88- and TRIF dependent signaling pathways
Authors: Díaz-Dinamarca Diego A. , Salazar Michelle L. , Escobar Daniel F. , Castillo Byron N. , Valdebenito Bastián , Díaz Pablo , Manubens Augusto , Salazar Fabián , Troncoso Mayarling F. , Lavandero Sergio , Díaz Janepsy , Becker María Inés , Vásquez Abel E.
Published: September 2023

The development of vaccine adjuvants is of interest for the management of chronic diseases, cancer, and future pandemics. Therefore, the role of Toll-like receptors (TLRs) in the effects of vaccine adjuvants has been investigated. TLR4 ligand-based adjuvants are the most frequently used adjuvants for human vaccines. Among TLR family members, TLR4 has unique dual signaling capabilities due to the recruitment of two adapter proteins, myeloid differentiation marker 88 (MyD88) and interferon-β adapter inducer containing the toll-interleukin-1 receptor (TIR) domain (TRIF). MyD88-mediated signaling triggers a proinflammatory innate immune response, while TRIF-mediated signaling leads to an adaptive immune response. Most studies have used lipopolysaccharide-based ligands as TLR4 ligand-based adjuvants; however, although protein-based ligands have been proven advantageous as adjuvants, their mechanisms of action, including their ability to undergo structural modifications to achieve optimal immunogenicity, have been explored less thoroughly. In this work, we characterized the effects of two protein-based adjuvants (PBAs) on TLR4 signaling via the recruitment of MyD88 and TRIF. As models of TLR4-PBAs, we used hemocyanin from Fissurella latimarginata (FLH) and a recombinant surface immunogenic protein (rSIP) from Streptococcus agalactiae. We determined that rSIP and FLH are partial TLR4 agonists, and depending on the protein agonist used, TLR4 has a unique bias toward the TRIF or MyD88 pathway. Furthermore, when characterizing gene products with MyD88 and TRIF pathway-dependent expression, differences in TLR4-associated signaling were observed. rSIP and FLH require MyD88 and TRIF to activate nuclear factor kappa beta (NF-κB) and interferon regulatory factor (IRF). However, rSIP and FLH have a specific pattern of interleukin 6 (IL-6) and interferon gamma-induced protein 10 (IP-10) secretion associated with MyD88 and TRIF recruitment. Functionally, rSIP and FLH promote antigen cross-presentation in a manner dependent on TLR4, MyD88 and TRIF signaling. However, FLH activates a specific TRIF-dependent signaling pathway associated with cytokine expression and a pathway dependent on MyD88 and TRIF recruitment for antigen cross-presentation. Finally, this work supports the use of these TLR4-PBAs as clinically useful vaccine adjuvants that selectively activate TRIF- and MyD88-dependent signaling to drive safe innate immune responses and vigorous Th1 adaptive immune responses.