bluuebunny/crossref_metadata_2025 huggingface dataset:
Geochemistry of Riphean terrigenous rocks in the Southern Urals and Siberia and variations of the continental-crust maturity
Authors: Nozhkin A.D. , Maslov A.V. , Podkovyrov V.N. , Turkina O.M. , Letnikova E.F. , Ronkin Yu.L. , Krupenin M.T. , Dmitrieva N.V. , Gareev E.Z. , Lepikhina O.P.
Published: February 2009

We consider the general and specific features of the evolution of the composition of fine-grained terrigenous rocks in the Riphean sedimentary megasequences of the Southern Urals, Uchur-Maya region, and Yenisei Ridge. It has been established that the crust on the southwestern (in the modern frame of references) periphery of the Siberian craton was geochemically the most mature segment of the Riphean continental crust. For example, the fine-grained clastic rocks and metapelites of all Riphean lithostratigraphic units of the Yenisei Ridge have higher median contents of Th than the most mature Paleoproterozoic crust, and in median contents of Y and Cr/Th values they are the most similar to it. In the Southern Urals and Uchur-Maya region, some units of the Riphean sedimentary sequences show median contents of Y and Th and Cr/Th values close to those of primitive Archean crust. Analysis of Cr/Th variations in the fine-grained terrigenous rocks of all three megasequences shows that the minimum Cr/Th values, evidencing a predominance or the abundance of felsic rocks in provenances, are typical of the Riphean argillaceous shales and metapelites of the Yenisei Ridge. The distinct Cr/Th and Cr/Sc increase in the fine-grained clastic rocks of the Chingasan Group of the ridge reflects the large-scale destruction of continental crust during the formation of rift troughs as a result of the Rodinia breakup in the second half of the Late Riphean. The Cr/Th variations in the Lower and Middle Riphean argillaceous shales and mudstones of the Bashkirian mega-anticlinorium and Uchur-Maya region are in agreement, which evidences the subglobal occurrence of rifting in the early Middle Riphean (so-called “Mashak rifting”).
The paleogeographic and stratigraphic confinement of giant floods in West Siberia in the Late Neopleistocene–Holocene
Authors: Zol’nikov I.D. , Gus’kov S.A.
Published: February 2009

The concepts of the role of catastrophic breakthroughs of ice- and rock-dammed and thermokarst lakes in West Siberia in the Late Neopleistocene–Holocene are systematized. The Late Neopleistocene glacial maximum in the mountains and on the plain was obviously at the same time, at 90–60 ka. It has been revealed that the basal part of the Late Quaternary cyclic three-stage upper Ob’ River terrace is formed by catafluvial sediments including boulder-gravels, which descend from the valley edge beneath the water line. The Early Karginian (Kharsoimian) marine layers are spatially related to the valleys of the rushed waters of ice-dammed Lake Ermakovskoe. Substantiation is given to the concept of catastrophic flows that arrived at the plain from the Pamir and Tien Shan mountains in the Holocene Optimum and carried the Aral microfauna through the Turgai trough into the Lake Chany area. Floods resulted from the breakthrough of thermokarst lakes in the north of the West Siberian plain were typical in Karginian and Holocene time. The breakthroughs of moraine-dammed basins in the Altai Mountains took place mainly in Karginian time, whereas the breakthroughs of rock-dammed lakes, in the Sartan and Holocene epochs.
Anti-inflammatory effect of lycopene on carrageenan-induced paw oedema and hepatic ischaemia–reperfusion in the rat
Authors: Bignotto Letícia , Rocha João , Sepodes Bruno , Eduardo-Figueira Maria , Pinto Rui , Chaud Marco , de Carvalho João , Moreno Heitor , Mota-Filipe Helder
Published: February 2009

The regular intake of tomatoes or its products has been associated with a reduced risk of chronic diseases and these effects have been mainly attributed to lycopene. Here, we evaluated the anti-inflammatory properties of lycopene and its protective effects on organ injury in two experimental models of inflammation. In order to study the effects of lycopene in local inflammation, a carrageenan-induced paw oedema model in rats was performed. Lycopene was administered as an acute (1, 10, 25 or 50 mg/kg, intraperitoneally, 15 min before carrageenan injection) and chronic treatment (25 or 50 mg/kg per d, 14 d). Inflammation was assessed by the measurement of paw volume increase after 6 h. Lycopene significantly inhibited paw oedema formation at two doses (25 and 50 mg/kg) in both acute and repeated administration. The effect of lycopene on liver inflammation was evaluated in a liver ischaemia–reperfusion (I/R) model. Rats were subjected to 45 min of ischaemia of three-quarters of the liver followed by 2 h of reperfusion. In this model, lycopene was administered daily at two doses (25 and 50 mg/kg) during the 14 d that preceded the experiments. Repeated administration of lycopene reduced liver injury induced by I/R, as demonstrated by the reduction of the increase in liver injury markers (aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase and γ-glutamyl transferase) and attenuation of liver tissue lipoperoxidation was evidenced by a decrease in malondialdehyde production. The present results show that lycopene exhibited local anti-inflammatory activity and also attenuated liver injury induced by I/R. We speculate that lycopene administration might be useful in the pharmacological modulation of inflammatory events.
The effects of different prey regimes on the proteolytic digestion of nymphs of the spined soldier bug, Podisus maculiventris (Hemiptera: Pentatomidae)
Authors: Pascual-Ruiz S. , Carrillo L. , Álvarez-Alfageme F. , Ruíz M. , Castañera P. , Ortego F.
Published: February 2009

The effects of different prey regimes on the performance and digestive physiology of the spined soldier bug, Podisus maculiventris (Say) (Hemiptera: Pentatomidae), were assessed. Specifically, P. maculiventris nymphs were fed on Colorado potato beetle (CPB), Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), larvae; Egyptian cotton leafworm (ECW); Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae); larvae; Calliphora spp. (CAL) (Diptera: Calliphoridae) pupae or a mixture of the three prey. No differences in development and weight gain were observed when P. maculiventris nymphs were fed different prey species (CPB, ECW or CAL). However, an increase in weight gain and a reduction in the duration of the stadia were observed for nymphs fed with a mixture of the three prey. To investigate the physiological background, biochemical analysis were carried out on insects dissected at the end of the feeding assay. We have found that the proteolytic activity in the salivary glands of P. maculiventris nymphs was not affected by prey species, whereas the relative activity of these proteases in the midgut depends on the prey. Moreover, gel assays proved that the proteolytic profiles of midguts from P. maculiventris nymphs feeding on CPB, ECW and CPB closely resembled those of their prey. All together, these results suggest that P. maculiventris may utilize enzymes from the prey they consume that may facilitate the process of digestion.
The biology ofDactylopius tomentosus(Hemiptera: Dactylopiidae)
Authors: Mathenge C.W. , Holford P. , Hoffmann J.H. , Spooner-Hart R. , Beattie G.A.C. , Zimmermann H.G.
Published: February 2009

Dactylopius tomentosus(Lamarck) (Hemiptera: Dactylopiidae) is a cochineal insect whose host range is restricted toCylindropuntiaspecies (Caryophyllales: Cactaceae). This insect has been utilized successfully for biological control ofCylindropuntia imbricata(Haw.) F.M. Knuth in Australia and South Africa. Despite this, its biology has not been studied previously, probably due to the widely held belief that the biology of allDactylopiusspecies is similar. This study investigated the life cycle and the morphological and reproductive characteristics ofD. tomentosus. Results revealed some unique characteristics ofD. tomentosus: (i) eggs undergo a much longer incubation period, an average of 17 days compared to <1 day in its congeners; (ii) eggs are laid singly but are retained as an egg mass secured in a mesh of waxy threads attached to the female; (iii) the developmental times of males and females are longer compared to otherDactylopiusspp. due to a longer egg incubation period; (iv)D. tomentosusdoes not undergo parthenogenesis; (v)D. tomentosusis smaller in size than its congeners; and (vi) male mating capacity and reproductive potential were both high and variable between males. There was a significant, strong, positive relationship (r=0.93) between female mass and fecundity, whereas the relationship between the number of females mated per male that became gravid and their fecundity was negative (r=−0.68). Besides contributing to our knowledge of this economically important species, the finding of unique characteristics ofD. tomentosusbiology underlines the need to study each species in this genus.
Teenage Mothers' Experiences of Formal Support Services
Authors: YARDLEY ELIZABETH
Published: February 2009

This article explores support services for teenage mothers. It draws upon empirical evidence from a wider study into the experiences of teenage mothers in the Midlands area of the UK, in which 20 young women who had given birth as teenagers participated in semi-structured interviews about their experiences of support services. The findings are used to make suggestions as to policy regarding support for teenage mothers, arguing that the diverse nature of support needs must be taken into account when designing support services. The article concludes that more attention should be paid to the possibilities of the group mode of support, rather than focusing on support delivered on a one-to-one basis, and, in addition, the functions of support services, beyond encouraging re-engagement in education, employment and training, need to be valued more significantly.
Remodelling the Third Sector: Advancing Collaboration or Competition in Community-Based Initiatives?
Authors: MILBOURNE LINDA
Published: February 2009

In the last decade, UK public agencies have increasingly been required to collaborate with non-state providers to deliver welfare services. Third sector organisations are now providers of services from early years to old age, taking a growing role in children and young people's services in socially deprived neighbourhoods. National policy has recognised third sector expertise in working with marginal groups of people. However, changing relationships with the state have drawn community organisations into new, often uncomfortable, organisational arrangements, affecting their work and their roles in relation to service users and community stakeholders.

This article examines recent changes from a third sector perspective, drawing on data from a study of community-based organisations providing children and young people's services in deprived localities. It considers the changing environment of ‘new localism’ affecting these organisations, focusing on recent plans for local area commissioning of services.

The article identifies some progress in supporting community services in deprived areas but illustrates how the continuing emphasis on competitive contracts and centrally driven frameworks undermines collaborative work and community trust. It argues that such mechanisms may serve short-term state interests but devalue the very community-level work, which is increasingly being promoted to address challenging social problems.
Factors related to the carriage ofVerocytotoxigenic E. coli,Salmonella, thermophilicCampylobacterandYersinia enterocoliticain cattle, sheep and pigs at slaughter
Authors: MILNES A. S. , SAYERS A. R. , STEWART I. , CLIFTON-HADLEY F. A. , DAVIES R. H. , NEWELL D. G. , COOK A. J. C. , EVANS S. J. , SMITH R. P. , PAIBA G. A.
Published: February 2009

A 12-month abattoir study was undertaken from January 2003. We collected 7492 intestinal samples from cattle, sheep and pigs at slaughter. Rectal samples were taken from cattle and sheep and caecal samples from pigs. They were examined for verocytotoxigenicE. coli(VTEC) O157,Salmonella, thermophilicCampylobacterandYersinia enterocolitica. Data were collected on the animal from which the sample came and this information was analysed to look at potential risk factors for carriage of these organisms. Logistic regression models were run where an adequate number of positive results were available. This revealed that VTEC O157 carriage in cattle was associated with the summer period and that age was a protective factor.Salmonellacarriage in pigs was associated with lairage times >12 h, the North East and not feeding when there was no bedding available. In cattle, carriage was associated with the summer period, the Eastern region of GB and dairy animals. In sheep a spring seasonal effect was seen, which coincided with the lambing period. The carriage of thermophilicCampylobacterin cattle was associated with single-species abattoirs, with age a protective factor. In sheep, winter was a risk period with lairage management influential. For pigs, lairage times of <12 h were found to be associated with carriage. A seasonal trend for carriage ofY. enterocoliticain all species was demonstrated with the period December–May a risk. For cattle, age was also a risk factor; for sheep feeding in the lairage and for pigs being held overnight were risk factors.
The effect of open-air factors on the virulence and viability of airborneFrancisella tularensis
Authors: HOOD A. M.
Published: February 2009

Unidentified open-air factors (OAFs) found to be adverse to the survival of microorganisms suspended on microthreads were investigated for their effect on realistic aerosols ofFrancisella tularensisin an open-air environment. This organism was chosen because it is probably the most infectious organism known to be capable of infecting both animals and man via the respiratory route, hence its potential use as a bioterrorist agent. A direct correlation was found between an open-air adverse effect on viability and virulence of airborne particles of <3 μm via the respiratory route in guinea pigs. One viable organism was sufficient to initiate an infection that resulted in a fatal tularaemia infection. The lethal effect of OAFs onF. tularensiswas found to vary from day to day and was related to the source of the air in the UK. The adverse effect on viability was associated with an inverse effect according to the size of the airborne particle.
Vision, eye movements, and natural behavior
Authors: LAND MICHAEL F.
Published: February 2009

Historically, the principal function of vision has been to provide the information needed to support action. Visually mediated actions rely on three systems: the gaze system responsible for locating and fixating task-relevant objects, the motor system of the limbs to carry out the task, and the visual system to supply information to the other two. All three systems are under the control of a fourth system, the schema system, which specifies the current task and plans the overall sequence of actions. These four systems have separate but interconnected cortical representations. The way these systems interact in time and space is discussed here in relation to two studies of the gaze changes and manipulations made during two ordinary food preparation tasks. The main conclusions are that complex action sequences consist of a succession of individual object-related actions, each of which typically involve a turn toward the object (if needed), followed by fixation and finally manipulation monitored by vision. Gaze often moves on to the next object just before manipulation is complete. Task-irrelevant objects are hardly ever fixated, implying that the control of fixation comes principally from top-down instructions from the schema system, not bottom-up salience. Single fixations have identifiable functions (locating, directing, guiding, and checking) related to the action to be taken. Several variants of the basic object-related action scheme are discussed, including single-action events in ball sports involving only one anticipatory gaze shift, continuous production loops in text and music reading, and storage–action alternation in copying tasks such as portrait sketching.
Efficient coding correlates with spatial frequency tuning in a model of V1 receptive field organization
Authors: WILTSCHUT JAN , HAMKER FRED H.
Published: February 2009

Efficient coding has been proposed to play an essential role in early visual processing. While several approaches used an objective function to optimize a particular aspect of efficient coding, such as the minimization of mutual information or the maximization of sparseness, we here explore how different estimates of efficient coding in a model with nonlinear dynamics and Hebbian learning determine the similarity of model receptive fields to V1 data with respect to spatial tuning. Our simulation results indicate that most measures of efficient coding correlate with the similarity of model receptive field data to V1 data, that is, optimizing the estimate of efficient coding increases the similarity of the model data to experimental data. However, the degree of the correlation varies with the different estimates of efficient coding, and in particular, the variance in the firing pattern of each cell does not predict a similarity of model and experimental data.
‘Prenasalised consonants’ and syllable timing: evidence from Runyambo and Luganda
Authors: Hubbard Kathleen
Published: February 2009

An interesting feature of many Bantu languages is the presence of what have been called ‘prenasalised consonants’-these are typically sounds that might be interpreted as a sequence of nasal + stop, but which behave in many respects like single segments:
Regular-expression derivatives re-examined
Authors: OWENS SCOTT , REPPY JOHN , TURON AARON
Published: February 2009

Regular-expression derivatives are an old, but elegant, technique for compiling regular expressions to deterministic finite-state machines. It easily supports extending the regular-expression operators with boolean operations, such as intersection and complement. Unfortunately, this technique has been lost in the sands of time and few computer scientists are aware of it. In this paper, we reexamine regular-expression derivatives and report on our experiences in the context of two different functional-language implementations. The basic implementation is simple and we show how to extend it to handle large character sets (e.g., Unicode). We also show that the derivatives approach leads to smaller state machines than the traditional algorithm given by McNaughton and Yamada.
AN EIGHTH CENTURY ARABIC TREATISE ON THE COLOURING OF GLASS: KITĀB AL-DURRA AL-MAKNŪNA (THE BOOK OF THE HIDDEN PEARL) OF JĀBIR IBN ḤAYYĀN (c. 721–c. 815)
Authors: AL-HASSAN AHMAD Y.
Published: February 2009

This paper examines the history of glass colouring. It reviews Kitāb al-Durra al-maknūna of Jābir ibn Ḥayyān, which deals with the subject. The manuscript of this practical treatise was discovered recently. Part one of the paper deals with Jābir as a philosopher and chemist. The art of lustre-painting on glass originated in Syria during the Umayyad Caliphate in the eighth century and was soon practised in the neighbouring area. The paper reviews Arabic literature that deals with the colouring of glass until the 13th century, and with pre-Islamic and Latin books of recipes that deal with glass colouring. Recipes for cast coloured glass are very few and scant in non-Arabic literature, and lustre-painting on glass was not mentioned in any treatise outside Arabic, even in the works of Theophilus and Neri. The colouring of glass gemstones by colour diffusion is not mentioned also. The paper compares the recipes of Kitāb al-Durra with the results of modern analysis of existing Islamic stained glass objects. There is a close correspondence, and the main indispensable ingredients in both cases are silver and copper compounds. Part one ends with an account of lāzaward as cobalt oxide in glass colouring. Part two of the paper gives a representative selection of recipes from Kitāb al-Durra for the three methods of glass colouring.
Funerals As Feasts: Why Are They So Important?
Authors: Hayden Brian
Published: February 2009

Extremely lavish funeral feasts are common and expensive in many village and chiefdom societies. Anthropologists and politicians often explain such apparently economically irrational expenditures in terms of culture values or traditions. However, viewed from the broader perspective of other types of promotional feasts in transegalitarian or more complex societies (including marriages and house feasts), overtly competitive funeral feasts are used to advertise the success of the surviving family and kin groups. This socioeconomic promotion is important for attracting powerful/successful/wealthy families as affines and allies. Such allies are critical for defending family and individual self-interests in village political and economic struggles that are endemic. Funerals are especially apt contexts for these displays and political manoeuvres.
Hohlenstein-Stadel and the Evolution of Human Conceptual Thought
Authors: Wynn Thomas , Coolidge Frederick , Bright Martha
Published: February 2009

Cognitive neuroscience provides a powerful perspective on the brain and cognition from which archaeologists can begin to document the evolution of the human mind. The following essay uses the Hohlenstein-Stadel figurine as a starting point to demonstrate the two kinds of conclusion open to an evolutionary cognitive archaeology: first, describing features of the cognitive life-world at specific points in human evolution, in this case central Europe 32,000 years ago, and second identifying the evolutionary timing and contexts for specific cognitive abilities, in this case various components of concept formation. We argue that the abstract concept underpinning the Hohlenstein-Stadel figurine resulted initially from an effortful (attentive) linking of ‘animal’ and ‘person’ concepts via the working memory network of the frontal and parietal lobes. These ‘animal’ and ‘person’ concepts themselves were largely unconscious folk biological categories generated by a parietal network that had evolved earlier, probably by the time of the earliest Homo sapiens. These in turn rest on even older, basic ontological categories of ‘animate’ and ‘manipulable’ objects that are temporal lobe networks, and which evolved much earlier still, perhaps with the advent of Homo erectus.
The neuropsychological profile of a subclinical obsessive-compulsive sample
Authors: KIM MYUNG-SUN , JANG KYOUNG-MI , KIM BIT-NA
Published: February 2009

We investigated the neuropsychological profile of subjects in a subclinical obsessive-compulsive disorder (OCD) sample. Psychometrically defined subclinical obsessive-compulsive (n = 21) and control (n = 22) subjects were examined. Comprehensive neuropsychological tests evaluating verbal/nonverbal memory, attention, and executive function were administered. The subclinical obsessive-compulsive group showed poorer performances on the Wisconsin Card Sorting Test (WCST), F(1, 41) = 13.80, p < .001, and Trail-Making Test (TMT), F(1, 41) = 5.48, p < .05, compared with the control group. The subclinical obsessive-compulsive group showed higher rates of total errors, perseverative errors, and perseverative responses. In addition, the subclinical obsessive-compulsive group committed a greater number of errors in the TMT. However, the groups showed no performance differences in the TMT after controlling for the effects of depression and anxiety, F(1, 39) = 0.11, p = .739. These results suggest that subclinical obsessive-compulsives seemed to display deficits in executive functioning. This neuropsychological profile is consistent with current theories proposing that executive dysfunction may serve as the pathophysiological mechanism underlying the development of obsessive-compulsive disorder. (JINS, 2009, 15, 286–290.)
The effects of treatment for posterior fossa brain tumors on selective attention
Authors: MABBOTT DONALD J. , SNYDER JANICE J. , PENKMAN LOUISE , WITOL ADRIENNE
Published: February 2009

We sought to identify whether deficits in selective attention are present in pediatric brain tumor patients. Selective attention was assessed with covert-orienting, filtering, and visual-search tasks in 54 patients with either (1) posterior fossa (PF) tumors treated with cranial radiation and surgery (n = 22); (2) PF tumors treated with surgery alone (n = 17); or (3) non-CNS tumors (n = 15), who served as a patient control group. To account for normal development, patient performance was also compared with that of healthy age-matched controls (n = 10). We found that in PF tumor patients selective attention was impaired, regardless of whether they were treated with cranial radiation and surgery or surgery alone. However, patients treated with cranial radiation were most impaired. These patients may have greater damage to posterior brain regions know to mediate selective attention as the result of tumor location, effects of surgery, and higher doses of radiation to the posterior regions of the brain. These findings help to elucidate the potential impact of pediatric brain tumors and their treatment on discrete attentional skills. (JINS, 2009, 15, 205–216.)
Progressive decline of decision-making performances during multiple sclerosis
Authors: SIMIONI SAMANTA , RUFFIEUX CHRISTIANE , KLEEBERG JOERG , BRUGGIMANN LAURE , DU PASQUIER RENAUD A. , ANNONI JEAN-MARIE , SCHLUEP MYRIAM
Published: February 2009

The purpose of this study was to evaluate longitudinally, using the Iowa Gambling Task (IGT), the dynamics of decision-making capacity at a two-year interval (median: 2.1 years) in a group of patients with multiple sclerosis (MS) (n = 70) and minor neurological disability [Expanded Disability Status Scale (EDSS) ≤ 2.5 at baseline]. Cognition (memory, executive functions, attention), behavior, handicap, and perceived health status were also investigated. Standardized change scores [(score at retest-score at baseline)/standard deviation of baseline score] were computed. Results showed that IGT performances decreased from baseline to retest (from 0.3, SD = 0.4 to 0.1, SD = 0.3, p = .005). MS patients who worsened in the IGT were more likely to show a decreased perceived health status and emotional well-being (SEP-59; p = .05 for both). Relapsing rate, disability progression, cognitive, and behavioral changes were not associated with decreased IGT performances. In conclusion, decline in decision making can appear as an isolated deficit in MS. (JINS, 2009, 15, 291–295.)
Long-term cognitive function following chemotherapy in patients with testicular cancer
Authors: PEDERSEN ANDERS DEGN , ROSSEN PHILIP , MEHLSEN MIMI YUNG , PEDERSEN CHRISTINA GUNDGAARD , ZACHARIAE ROBERT , VON DER MAASE HANS
Published: February 2009

Cancer patients frequently report cognitive complaints following chemotherapy, but the results from the available studies, mainly of women with breast cancer, are inconsistent. Our aim was to compare cognitive function of men with testicular cancer (TC) who had orchiectomy and chemotherapy (bleomycin, etoposide, cisplatin) with men who had orchiectomy only or orchiectomy and radiotherapy. Thirty-six chemotherapy patients and 36 nonchemotherapy patients were tested 2–7 years after treatment for TC with standardized neuropsychological tests. Chemotherapy and nonchemotherapy patients displayed similar performances on cognitive tests (p values adjusted for multiple comparisons: .63–1.00). Moreover, there was no difference in the proportion of cognitively impaired patients in the chemotherapy group (5.6%) compared to the nonchemotherapy group (8.3%) (χ2 = 0.22, p = .64). Our results are discordant with previous findings indicating cognitive impairment following chemotherapy and suggest that TC patients do not need to fear long-term cognitive consequences following chemotherapy. (JINS, 2009, 15, 296–301.)
Neurocognitive differences between pedophilic and nonpedophilic child molesters
Authors: SUCHY YANA , WHITTAKER J. WILSON , STRASSBERG DONALD S. , EASTVOLD ANGELA
Published: February 2009

Although some evidence exists that child molesters may be characterized by structural and functional brain abnormalities, findings across studies are inconsistent. Past cognitive research in this area has been extensively criticized for relying on conceptually weak batteries, measures of questionable reliability, and poorly defined samples (i.e., failing to distinguish between pedophilic and nonpedophilic child molesters). The present study aimed to address the weaknesses of past research by comparing 40 child molesters (20 pedophilic and 20 nonpedophilic) and 20 demographically matched nonoffender controls on six well-defined neurocognitive composite scores of comparable reliability (i.e., semantic knowledge, executive functioning, processing speed, motor speed, auditory memory, and visual memory). Results indicated that pedophilic child molesters exhibit slower processing speed, nonpedophilic child molesters exhibit poorer semantic knowledge, and both molester groups exhibit executive weaknesses as compared to nonoffender controls. This study is the first to compare the two molester types on neurocognitive functions. The observed differences between the molester groups help explain inconsistencies in past research and demonstrate the need to distinguish between the two types of child molesters when studying neurobiologic underpinnings of sexual offending. (JINS, 2009, 15, 248–257.)
Injection of bleomycin in newborn mice induces autoimmune sialitis that is transferred by CD4 T cells
Authors: Ishikawa Hideaki , Ito Sachiko , Nishio Naomi , Yuzawa Yukio , Matsuo Sei‐ichi , Isobe Ken‐ichi
Published: February 2009

Bleomycin (BLM) induces cellular apoptosis or necrosis by producing reactive oxygen species, and has been used to induce scleroderma in adult mice. We wondered whether BLM induces the same pathological phenotype in newborn mice as in adult mice. BLM was subcutaneously administrated to newborn BALB/c mice. At 1 month of age, BLM‐treated mice showed severe destruction of salivary glands with enlargement of nearby lymph nodes. These nodes contained CD4+ T cells and B220+cells with high expression of MHC class II molecules. In addition, autoantibodies were detected by HEp‐2 staining and western blotting. The cell transfer experiments were performed to evaluate the role of autoimmune phenomena in these pathological changes. Following the transfer of enriched CD4+ T cells to 1‐month‐old BALB/c nude mice, the salivary glands were severely damaged with CD4+ T cell and B220+ cells infiltrations. The number of T‐cell antigen receptor Vβ 8.3+ CD4+ T cells was significantly increased in BLM‐treated murine spleen. These findings will provide new insights into the causal factors of environment in autoimmunity and the relationship between autoreactive CD4+ T cells and autoantibodies.
Guidelines for Achieving High Accuracy in Isotope Dilution Mass Spectrometry (IDMS)
Authors: Bedson Peter
Published: February 2009

The isotope dilution mass spectrometry (IDMS) technique is well known and widely reported in the literature. However, its application can present considerable difficulties with regard to obtaining reliable results. Produced jointly by the Royal Society of Chemistry's Analytical Methods Committee and the Valid Analytical Measurement (VAM) programme, the aim of this book is to provide a simplified yet robust methodology, together with adequate guidance, to enable laboratories wishing to use the technique to obtain reliable data. The methodologies, for inorganic and organic mass spectrometry, which use exact and approximate matching, are illustrated with worked examples and clear diagrammatic representations. A comprehensive glossary of terms, references to key publications and an extensive IDMS bibliography are also provided. Clear and comprehensive in coverage, Guidelines for Achieving High Accuracy in Isotope Dilution Mass Spectrometry (IDMS) will provide valuable assistance to a wide variety of analytical chemists interested in applying the IDMS technique to their own measurement applications.
Co-activator SRC-1 is dispensable for transcriptional control by STAT3
Authors: Cvijic Helena , Bauer Kay , Löffler Dennis , Pfeifer Gabriele , Blumert Conny , Kretzschmar Antje K. , Henze Christian , Brocke-Heidrich Katja , Horn Friedemann
Published: February 2009

SRC (steroid receptor co-activator)-1 has been reported to interact with and to be an essential co-activator for several members of the STAT (signal transducer and activator of transcription) family, including STAT3, the major signal transducer of IL (interleukin)-6. We addressed the question of whether SRC-1 is crucial for IL-6- and STAT3-mediated physiological responses such as myeloma cell survival and acute-phase protein induction. In fact, silencing of SRC-1 by RNA interference rapidly induced apoptosis in IL-6-dependent INA-6 human myeloma cells, comparable with what was observed upon silencing of STAT3. Using chromatin immunoprecipitation at STAT3 target regions of various genes, however, we observed constitutive binding of SRC-1 that decreased when INA-6 cells were treated with IL-6. The same held true for STAT3 target genes analysed in HepG2 human hepatocellular carcinoma cells. SRC-1-knockdown studies demonstrated that STAT3-controlled promoters require neither SRC-1 nor the other p160 family members SRC-2 or SRC-3 in HepG2 cells. Furthermore, microarray expression profiling demonstrated that the responsiveness of IL-6 target genes is not affected by SRC-1 silencing. In contrast, co-activators of the CBP [CREB (cAMP-response element-binding protein)-binding protein]/p300 family proved functionally important for the transactivation potential of STAT3 and bound inducibly to STAT3 target regions. This recruitment did not depend on the presence of SRC-1. Altogether, this suggests that functional impairment of STAT3 is not involved in the induction of myeloma cell apoptosis by SRC-1 silencing. We therefore conclude that STAT3 transactivates its target genes by the recruitment of CBP/p300 co-activators and that this process generally does not require the contribution of SRC-1.
Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP
Authors: Park Soo-Jung , Kim Mi-Ju , Kim Hak-Bong , Kang Chi-Dug , Kim Sun-Hee
Published: February 2009

Resistance to imatinib is commonly associated with reactivation of Bcr-Abl signalling. However, Bcr-Abl-independent signalling pathways may be activated and contributed to imatinib resistance in some CML (chronic myelogenous leukaemia) patients. We had isolated three imatinib-resistant K562/R1, R2 and R3 variants with gradual loss of Bcr-Abl from K562 cells to develop effective therapeutic strategies for imatinib-resistant CML. Interestingly, we found that these cells became highly sensitive to TRAIL (tumour necrosis factor-related apoptosis-inducing factor) in comparison with K562 cells showing high resistance to TRAIL. Treatment of K562/R3 cells with TRAIL resulted in activation of TRAIL receptor pathway by including caspase 8 activation, Bid cleavage, cytochrome c release and caspase 3 activation. These results were accompanied by down-regulation of c-FLIP {cellular FLICE [FADD (Fas-associated death domain)-like interleukin 1β-converting enzyme]-inhibitory protein} in imatinib-resistant K562 variants compared with K562 cells. Overexpression of c-FLIP in K562/R3 cells acquired TRAIL resistance and conversely, c-FLIP-silenced K562 cells became sensitive to TRAIL. Moreover, Bcr-Abl-silenced K562 cells showed down-regulation of c-FLIP and the subsequent overcome of TRAIL resistance. Taken together, our results demonstrated for the first time that the loss of Bcr-Abl in imatinib-resistant cells led to the down-regulation of c-FLIP and subsequent increase of TRAIL sensitivity, suggesting that TRAIL could be an effective strategy for the treatment of imatinib-resistant CML with loss of Bcr-Abl.
Extraction of the anti-sepsis component from Terminaliachebula Retz and evaluation of its biological activities
Authors: Yao Jie , Zheng Jun Song , Fu WeiLing , Zheng Jiang
Published: February 2009

Many clinical experiments and studies have demonstrated that traditional Chinese medicines possess the capacity for being used in anti-sepsis. In this paper, we screened 78 herbs based on biosensor technology by targeting of lipid A. Terminaliachebula Retz was found to possess the highest capability of binding lipid A. With CER (cation-exchange resin) and HPLC, we obtained three active components extracted from Terminaliachebula Retz, and named them TCR1, TCR2 and TCR3 respectively. These three components were evaluated with the biosensor, and it was found that the TCR3 was the most capable candidate to bind lipid A. We also studied the biological activities of TCR3 against sepsis in vitro and in vivo. in vitro, TCR3 could significantly inhibit LPS (lipopolysaccharide)-induced LAL (Limulus amoebocyte lysate)) from agglutination and decrease TNFα (tumour necrosis factor α) release from RAW264.7 cells induced by LPS in a dose-dependent manner. in vivo, TCR3 could significantly protect mice against a lethal challenge with LPS and heat-killed Escherichia coli 35218 in a dose-dependent manner. These results demonstrate that Terminaliachebula Retz is an important herb to neutralize LPS and it has the potential to serve as a treatment for sepsis.
Effects of environmental conditions on the production of hypocotyl hairs in seedlings of Melaleuca ericifolia (swamp paperbark)
Authors: Robinson Randall W. , Boon Paul I. , Sawtell Nina , James Elizabeth A. , Cross Robert
Published: February 2009

The production of hypocotyl hairs in the early stages of seedling development can strongly influence the success with which plants recruit sexually in harsh environments. Although wetlands are one type of environment in which seedlings might be expected to develop hypocotyl hairs, there have been few studies of these structures in the woody aquatic plants. We investigated the production of hypocotyl hairs in Melaleuca ericifolia Sm., a small wetland tree widely distributed across swampy coastal areas of south-eastern Australia, in relation to water availability, salinity, temperature and light regime. Hypocotyl hairs were ~20 mm long × 30 μm wide; in contrast, root hairs were generally less than 5 mm long and 15 μm wide. Hypocotyl hairs were produced only under a narrow range of environmental conditions—low salinity, low water availability, moderate temperature, and darkness—and seedlings that failed to produce hypocotyl hairs did not survive. Since the conditions under which hypocotyl hairs were produced were at least as, and possibly even more, restricted than those required for successful germination of seeds, it is likely that the successful sexual recruitment of M. ericifolia would be rare and episodic under conditions existing in most coastal wetlands in south-eastern Australia.
Embryology of the dioecious Australian endemic Lomandra longifolia (Lomandraceae)
Authors: Ahmad Nabil M. , Martin Peter M. , Vella John M.
Published: February 2009

Microsporogenesis, embryogeny and endosperm development of Lomandra longifolia Labill. are described in detail. The formation of the anther wall is the basic type composed of four cell layers, namely an epidermis, an endothecium, one middle layer and a tapetum. The tapetum layer has glandular, uninucleate cells. Successive cytokinesis follows meiosis, subsequently forming a tetrahedral tetrad of microspores. The ovule in each carpel is hemitropous, crassinucellate and bitegmic, with the micropyle formed by the inner integument. The archesporial cell divides periclinally to form the primary parietal and primary sporogenous cells. The sporogenous cell functions as the megaspore mother cell, whereas the parietal cell divides to give rise to two parietal layers. The mature megagametophyte, which has enlarged synergids and antipodals, is of the Polygonum type, with the normal complement of seven cells and eight nuclei. Nucellar tissue in the mature ovule consists of enlarged dermal cells and irregular subdermal cells surrounding a central strand of markedly smaller cells. Endosperm development is of the nuclear type. Embryo development is of the Graminad type, characterised by oblique zygotic and early pro-embryonic divisions.
Simultaneous encapsulation of seed and mycorrhizal fungi for long-term storage and propagation of terrestrial orchids
Authors: Sommerville Karen D. , Siemon John P. , Wood Chris B. , Offord Catherine A.
Published: February 2009

Ex situ conservation of threatened terrestrial orchids requires the simultaneous conservation of their mycorrhizal associations. A method for encapsulating both seed and fungi in alginate beads (known as encapsulation–dehydration) was applied to the storage and propagation of two endangered orchid species in NSW, Australia—Pterostylis saxicola D.L.Jones & M.A.Clem. and Diuris arenaria D.L.Jones. We tested the effect of storage duration and temperature on fungal recovery and germination potential in vitro, and recorded survival for seedlings subsequently transferred to potting mix. Storage at 23°C significantly reduced fungal recovery and germination for both species after only 3 months (P < 0.05), whereas storage at 4°C significantly reduced fungal recovery for P. saxicola after 6 months (P < 0.05). Storage for 6 months at −18 and −196°C had no significant effect on the fungal recovery and germination percentages of either species. All beads transferred directly from in vitro culture to potting mix resulted in the establishment of at least one seedling, and production of a healthy tuberoid, when transferred near the commencement of the natural growing season. The encapsulation–dehydration method may have a practical application for use in ex situ conservation of other terrestrial orchids, as well as their mycorrhizal fungi.
Role of mucilage in germination of Dillenia indica (Dilleniaceae) seeds
Authors: Thapliyal Ramesh C. , Phartyal Shyam S. , Baskin Jerry M. , Baskin Carol C.
Published: February 2009

Dillenia indica Linn. is a nearly evergreen tree widely distributed in South-east Asia. Regeneration is by seeds borne within large indehiscent fruits. When extracted fresh, seeds are glued together by the sticky mucilage on the seedcoat. Mucilage anchors seeds during rains, thus preventing them from escaping the fruit. Mucilage does not promote or inhibit germination, but it does restrict the inflow of water into seeds during the initial stages of imbibition. In nature, seeds germinate (within fruits) in July–August following heavy rains that wash off most of the mucilage. Light had a significant effect on germination: a 12/12 h light/dark regime resulted in more and faster germination than when seeds were incubated in total darkness. Seeds exhibited one flush of germination within fruits and another flush in the laboratory following extraction, drying and rehydration, suggesting the occurrence of two physiological types of seeds with regard to light requirement for germination. The embryo of D. indica is underdeveloped, and seeds take ~30 days to germinate under appropriate conditions. Thus, the seeds have morphological dormancy (MD). The possible roles of seed-coat mucilage and light in the germination biology of D. indica seeds in nature are discussed.
Salsola tragus or S. australis (Chenopodiaceae) in Australia—untangling taxonomic confusion through molecular and cytological analyses
Authors: Borger Catherine P. D. , Yan Guijun , Scott John K. , Walsh Michael J. , Powles Stephen B.
Published: February 2009

Salsola tragus sensu lato (Chenopodiaceae) is found throughout Western Australia and is considered to be a weed in both natural and agricultural ecosystems, although the current taxonomic status of this species is not clear. The taxonomic literature reports morphological variation within Australian populations of the weed, indicating that there may be genetically distinct ecotypes or unidentified subspecies present within the species. A genetic and cytological approach was used to detect variation between 22 populations of S. tragus sensu lato in the south-west of Western Australia. Out-groups used in this study included a population of S. tragus L. from the USA and Maireana brevifolia (R.Br.) Paul G.Wilson (Chenopodiaceae) from Lake Grace. Four genetically distinct groups were identified, which were not closely related to the S. tragus out-group (~60% similarity). Further, these groups and a S. australis R.Br. sample from the USA were all diploid (2n = 18), unlike the tetraploid (2n = 36) S. tragus. The predominant wheatbelt weed, group A, which was previously classified as S. tragus ssp. tragus L., was identified as S. australis. This species is probably native to Australia, given its arrival predated European invasion. Further research is required to clarify the taxonomic status of the other three possible taxa and determine their status in relation to S. australis.
Condition of fenced and unfenced remnant vegetation in inland catchments in south-eastern Australia
Authors: Briggs Sue V. , Taws Nicola M. , Seddon Julian A. , Vanzella Bindi
Published: February 2009

Considerable areas of remnant native vegetation have been fenced in the last decade to manage grazing by domestic stock. This study investigated vegetation condition in comparative fenced and unfenced remnant vegetation in the mid–upper Murrumbidgee and Lachlan catchments in south-eastern Australia. Native species richness, native groundcover and overstorey regeneration were higher at fenced than at unfenced sites. Area of bare ground was lower at fenced sites. Exotic groundcover did not differ between fenced and unfenced sites. Native species richness was higher at sites fenced for longer and with no stock grazing; neither native nor exotic groundcover at fenced sites was related to time since fencing or stock grazing pressure. Some tree species regenerated at both fenced and unfenced sites (Blakely’s red gum, Eucalyptus blakelyi; tumbledown gum, E. dealbata, long-leaved box, E. goniocalyx; red stringbark, E. macrorhyncha), some regenerated at few fenced and few unfenced sites (white box, E. albens; yellow box, E. melliodora) and some regenerated at fenced sites but not at unfenced sites (grey box, E. microcarpa; mugga ironbark, E. sideroxylon; white cypress pine, Callitris glaucophylla). Although less robust than pre- and postfencing monitoring, the comparisons reported here provide a logistically feasible and relatively inexpensive assessment of effects of the sizeable public investment in fencing on vegetation condition.
Comparative study on epicuticular leaf waxes of Araucaria araucana, Agathis robusta and Wollemia nobilis (Araucariaceae)
Authors: Dragota Simona , Riederer Markus
Published: February 2009

The present study describes fine structure and chemical composition of the epicuticular leaf waxes of three Araucariaceae species. The leaf surfaces of samples from greenhouse-grown juvenile trees of Araucaria araucana K.Koch and Agathis robusta F.Muell. were investigated by scanning electron microscopy (SEM) and compared with data for a young greenhouse-grown Wollemia nobilis W.G.Jones, K.D.Hill & J.M.Allen cutting characterised earlier. The chemical compositions of the epicuticular waxes selectively removed from the adaxial and abaxial leaf surfaces of A. araucana, A. robusta and W. nobilis were studied by gas chromatography combined with mass spectroscopy (GC–MS). The main components of the leaf cuticular waxes of A. araucana, A. robusta and W. nobilis are members of the following three major compound classes: n-alkanes, secondary alcohols and alkane diols. It was shown earlier that the latter two classes contribute to the formation of the tubular epicuticular-wax crystals on the leaf surfaces. The present comparative study also revealed differences in the crystalline microstructure and chemical composition of the epicuticular leaf waxes among the three species.