bluuebunny/crossref_metadata_2025 huggingface dataset:
Propuesta de modelo de gestión dinámica de la cadena de producción de elementos prefabricados de concreto armado implementando herramientas informáticas integrales para reducir las pérdidas de independencia de sistemas en la construcción de edificaciones multifamiliares en Lima
Authors:   , Collantes Gallo Renzo Paolo , Portilla Orosco Jorge Antonio
Published: November 2021

En la siguiente investigación se busca impulsar al sector construcción en el Perú hacia las nuevas tendencias de construcción como metodologías de construcción, nuevas formas de gestionar un proyecto y llevar un control activo para acercar al sector a la industrialización de la construcción conocida como “Contruction 4.0”. Evidencia de ello es que ya se están realizando construcciones con elementos prefabricados que han garantizado la mejora en la calidad del producto, pero la forma de gestionar este nuevo método constructivo, y en general cualquier método, se encuentra aún deficiente generando ciertos conflictos en la cadena de producción de proyectos de construcción de edificaciones inmobiliarias que repercuten en tiempo y costo del proyecto. Se aplica una metodología de gestión utilizando dos herramientas informáticas vigentes que están dando excelentes resultados en países extranjeros que son el BIM y el ERP. Ambas se focalizan en la integración de la información y un trabajo dinámico constante para mejorar la interacción entre las partes involucradas. Se realizó una simulación con las herramientas para evaluar las mejoras que la metodología puede contribuir en la cadena de producción de elementos prefabricados comparando los resultados obtenidos con la ponderación de datos reales de 3 casos de estudio que se seleccionaron por presentar una tendencia en problemas con más recurrencia. Se evidenciará una reducción en tiempo y costo lo que genera altas expectativas en su aplicación en un proyecto real.
Mejora del transbordo Corredor Rojo-Metro de Lima por medio de un conector peatonal en la intersección de la Av. Javier Prado con Av. Aviación
Authors:   , Asenjo Albitres Christian , Tocas Morales Frank Anthony
Published: November 2021

La realidad peruana refleja una falta de integración de los sistemas de transporte. Asimismo, se evidencia bajos estándares de calidad con relación a las infraestructuras urbanas y una inseguridad hacia el peatón en la ciudad de Lima, un ejemplo de lo mencionado ocurre en la intersección de la Avenida Javier Prado con Aviación. Mediante el software Vissim-Visswalk 9 se realiza una microsimulación que refleja las trayectorias, frecuencias y composiciones vehiculares y peatonales, la cual se calibra y valida respectivamente. La metodología propuesta describe una delimitación de la intersección mencionada, luego se realiza la clasificación de usuarios de transportes públicos y tipos de vehículos para proceder al levantamiento de información dinámica en cuanto al aforo de vehículos y peatones y posteriormente registrarlas en el Vissim-Viswalk 9. De manera cualitativa, la seguridad vial se evalúa mediante la utilización de listas de chequeo las cuales reflejan claramente una inseguridad y alta vulnerabilidad hacia la integridad del peatón evidenciado en los 13 ítems analizados. La propuesta de solución consiste en el diseño de un conector peatonal de 320 metros, el cual cumple con los reglamentos requeridos ya que tiene un ancho efectivo constante de 1.6 metros y una pendiente máxima de 5% y 10% en el conector y en la rampa, respectivamente. Los resultados demuestran que la propuesta permite una reducción del tiempo de transbordo en un 17.1% y un aumento en la velocidad promedio de 26.1%, asimismo se evidencia un aumento significativo de la seguridad vial hacia los peatones.
Industrial brines of the Khoreyver depression of the Pech ora plate
Authors:   Institute of Geology FRC Komi SC UB RAS, Mityusheva T. P. , Amosova O. Ye.
Published: November 2021

We studied areal distribution of the Khoreyver depression and strontium and lithium-rich iodine-bromine and iodine-boron industrial brines in the hydrogeological section. We presented the potential of the territory for practical use of industrial sodium chloride and calcium-sodium underground brines in the maps of distribution of bromine, iodine, boron and strontium-lithium iodinebromine and iodine-boric industrial brines within three Paleozoic calcareous aquifers (O2–S–D1; D3–C1; C–P1). Separate areas with lithium-strontium iodine - bromine and iodine-boric standard quality brines are designated.
Trends in development of higher education in modern Russia
Authors:   Smolensk State University, Alieva E.S.
Published: November 2021

Examined are the main development trends in the field of higher education, the originality of their manifestation in Russian society. The specificity of the continuity of learning is revealed, the directions of transformation of the educational process in the context of digitalization are determined. The topic of the effectiveness of the implementation of innovative methods in educational activities, such as case-method, tutoring, interactive training, was touched upon. The duality of the study is noted, since the introduction of innovative methods into the educational activities of Russian universities, on the one hand, has a positive effect on the state of the educational system, on the other hand, it gives rise to a tendency towards an increase in social and digital inequality in education and society as a whole, and also presupposes changes in requirements. to the teaching staff, which is now required first of all to be motivated and ready to master and use modern innovative methods in educational practice.
Neisseria Mucosa Does Not Inhibit the Growth of Neisseria gonorrhoeae
Authors: Abdellati Said , Laumen Jolein , Gonzalez Natalia , Manoharan-Basil Sheeba , Van Dijck Christophe , De Baetselier Irith , Martiny Delphine , De Block Tessa , Kenyon Chris
Published: November 2021

Antibiotic-sparing treatments are required to prevent the further emergence of anti-microbial resistance in Neisseria gonorrhoeae. Commensal Neisseria species have previously been found to inhibit the growth of pathogenic Neisseria species. For example, a previous study found that 3 out of 5 historical isolates of Neisseria mucosa could inhibit the growth of N. gonorrhoeae. In this study, we used agar overlay assays to assess if 24 circulating and historical isolates of Neisseria mucosa could inhibit the growth of 28 circulating and historical isolates of N. gonorrhoeae. Although pitting around each colony of N. mucosa created an optical illusion of decreased growth of N. gonorrhoeae, we found no evidence of inhibition (n=24). In contrast, positive controls of Streptococcus pneumoniae and Escherichia coli demonstrated a strong inhibitory effect against the growth of N. gonorrhoeae.
Highly Adaptive Phenuiviridae with Great Biomedical Importance
Authors: Chen Ji-Ming , Sun Ming-Hui , Ji Yu-Fei , Li Guo-Hui , Chen Rui-Xu , Gong Huan-Yu
Published: November 2021

The newly established virus family Phenuiviridae in Bunyavirales harbors viruses infecting three kingdoms of host organisms (animals, plants, and fungi), which is rare in known virus families. Many phenuiviruses are arboviruses and replicate in two distinct hosts (e.g., insects and humans or rice). Multiple phenuiviruses, such as Dabie bandavirus, Rift Valley fever phlebovirus, and Rice stripe tenuivirus, are highly pathogenic to humans, animals, or plants. They impose heavy global burdens on human health, livestock industry, and agriculture and are research hotspots. In recent years the taxonomy of Phenuiviridae has been expanded greatly, and researches on phenuiviruses have made significant progress. With these advances, this review drew a novel panorama regarding the biomedical significance, distribution, morphology, genomics, taxonomy, evolution, replication, transmission, pathogenesis, and control of phenuiviruses, to aid researchers in various fields to recognize this highly adaptive and very important virus family.
Evaluation NO2 Detection Using Low-Cost Folded Path Photometer
Authors: Fatkhurrahman Januar Arif , Lestari Puji
Published: November 2021

Nitrogen dioxide (NO2) contributes to several impacts both for human health and the environment. Periodical measurement of NO2 at industrial and residential areas needs comprehensive and reliable instrumentation; long-interference-free measures with minimum maintenance and re-calibration. DOAS can be used as a direct measurement technique based on specific absorption characteristics of NO2 follow Beer-Lambert law. This research applies a low-cost folded path photometer for measurement NO2 in air. Cheap tubular acrylic used as a detection cell with a 3D printed framework makes it compact, modular, and flexible. Evaluation of the DOAS conducted by instrument test responses using NO2 gas. The estimated LOD was ~ 1263 pb using 2 nm resolution of the spectrometer and 6-meter detection cell length. Deviation of the DOAS estimated to be 0.8% at high concentration and 2.85% at a low concentration based on DOAS calibration. An intercomparison between methods used to evaluate instrument performance to measure NO2 using emission from a motorcycle with coefficient correlation (R) 0.649 for paired DOAS-ASTM D1607 Griesz Saltzmann Method 0.846 for paired DOAS-Electrochemical Gas Analyzer. This significant correlation is caused by different respond time between paired methods, while it is still comparable for NO2 measurement.
Electroanalytical Biosensors for Circulating Tumor DNA Detection—A Brief Review
Authors: Peng Zhijia , Lin Xiaogang , Nian Weiqi , Zheng Xiaodong , Wu Jayne
Published: November 2021

Early diagnosis and treatment have always been highly desired in the fight against cancer, and detection of circulating tumor DNA (ctDNA) has recently been touted as highly promising for early cancer screening. Consequently, the detection of ctDNA in liquid biopsy gains much attention in the field of tumor diagnosis and treatment, which has also attracted research interest from the industry. However, traditional gene detection technology is difficult to achieve low cost, real-time and portable measurement of ctDNA. Electroanalytical biosensors have many unique advantages such as high sensitivity, high specificity, low cost and good portability. Therefore, this review aims to discuss the latest development of biosensors for minimal-invasive, rapid, and real-time ctDNA detection. Various ctDNA sensors are reviewed with respect to their choices of receptor probes, detection strategies and figures of merit. Aiming at the portable, real-time and non-destructive characteristics of biosensors, we analyze their development in the Internet of Things, point-of-care testing, big data and big health.
Threshold of Reactivity and Tolerance to Precautionary Allergen-Labelled Biscuits of Baked Milk- And Egg-Allergic Children
Authors: Fierro Vincenzo , Marzano Valeria , Monaci Linda , Vernocchi Pamela , Mennini Maurizio , Valluzzi Rocco , Levi Mortera Stefano , Pilolli Rosa , Dahdah Lamia , Calandrelli Veronica , Bracaglia Giorgia , Arasi Stefania , Riccardi Carla , Fiocchi Alessandro , Putignani Lorenza
Published: November 2021

Extremely sensitive food-allergic patients may react to very small amounts of allergenic foods. Precautionary allergen labelling (PAL) warns from possible allergenic contaminations. We explored the ability of proteomic methods to identify minute amounts of milk/egg allergens in a brand of PAL-labelled milk- and egg-free biscuits. We evaluated the reactivity of children with severe milk and egg allergy, by oral food challenge. Traces of milk and/or egg allergens in biscuits were measured by two different liquid-chromatography-mass spectrometry methods. The binding of patient's serum with egg/milk proteins was assessed at immunoblotting. None of the patients reacted to biscuits. Egg and milk proteins were found under the limit of detection of 0.6 µg/g for milk and egg (method A), and of 0.1 and 0.3 µg /g for milk and egg, respectively (method B). The immunoblots did not show milk/egg proteins in the studied biscuits. Our biscuits did not contain allergens of clinical significance. Their milk/egg content is far lower than 4 µg of milk or egg protein per gram of product, the minimal doses considered theoretically capable of causing reactions. With high sensitivity, proteomic assessments predict the harmlessness of very small amount of allergenic foods and can be used to avoid unnecessary PAL.
Variation in Nutritional Components in Roots From Ahipa (Pachyrhizus ahipa (Wedd.) Parodi) Accessions and an Interspecific Hybrid (P. Ahipa × P. Tuberosus (Lam.) Spreng.)
Authors: Leidi-Montes Eduardo O. , Ech-Chliah Youssef , Rossini-Oliva Sabina , Sørensen Marten
Published: November 2021

Among the many neglected underutilized species, tuberous Andean root crops like the ahipas (Pachyrhizus ahipa) constitute a promising alternative for increasing diversity in nutrient sources and food security at a regional level. In this study, we present the content of some functional compounds in tuberous roots from several ahipa accessions and the progenies of the interspecific hybrid X207 (P. ahipa × P. tuberosus). A significant objective was to determine protein and free amino acids in the roots to evaluate their food quality as protein supply. The interspecific hybrids have been found to possess the root quality to provide the crop with a higher dry matter content. The high dry matter content of the P. tuberosus Chuin materials is retained in the root quality of the hybrids. Food functional components like carbohydrates, organic acids, and proteins were determined in several ahipa accessions and a stable (non-segregating) progeny of the interspecific hybrid, X207. The X207 roots showed a significantly higher dry matter content and a lower content in soluble sugars, but no significant differences were found in starch content or organic acids compared to the ahipa accessions. About the root mineral contents, Fe and Mn concentrations in X207 were significantly raised compared to the average of ahipa accessions. Among the ahipa and the hybrid, no prominent differences in protein content or protein amino acids were found, being both partially defective in providing sufficient daily intake of some essential amino acids. Root weight, a central component of root yield, was significantly higher in X207, but thorough field studies are required to substantiate the hybrid’s superior yield performance..
A Phone of One’s Own: Woolf, TikTok and the Aesthetic Evolution of Bisexuality
Authors: Wells Christopher James
Published: November 2021

Whereas bisexuality, as it existed in modernity, has been described as a ‘floating signifier', one that was problematically conflated with gender and intersex bodies, the articulation of bisexuality is now experiencing a discursive resurgence in spaces and platforms online. Through a deliberately disparate comparison between Virginia Woolf’s modernist writing and the discussions of bisexuality on the video-sharing social networking service TikTok, this essay presents a reflective reassessment of how far bisexual representation in the popular imagination has progressed and by extension, evaluate extant limitations. To realize these ambitions, I compare the reception of sexology (the new science of sexuality) in ‘high’ modernist literature with a post-modern demographic whose bisexuality is articulated in the 2020s online via TikTok’s towards what I would demarcate as a post-queer theory user base. This essay is not intended as an overview of the advancements made in psychoanalytic institutions about bisexuality nor does it set out to comment on the refinement of bisexuality’s aestheticization through time. Instead, it uses these two temporally specific moments in the cultural zeitgeist to compare and contrast how differently two different demographics articulate bisexuality, both as a written mode in modernism and as a visual apparatus online. This is less a critique of bi-erasure, but an interrogation of why and how bisexual representation, as an aestheticized subjectivity that compromises romantic, spiritual, and erotic desires for bodies of all genders, continues to be problematically restrictive.
Review Potensi Daun Awar-awar Sebagai Agen Ko-Kemoterapi Antimetastasis Kanker Payudara
Authors: Salsabila Dhiya Ulhaq , Faqiha Syifa Athia Zainun , Anggraeni Afivah Dewi , Ikawati Muthi'
Published: November 2021

Kematian akibat kanker payudara sebagian besar disebabkan oleh metastasis. Kemoterapi sebagai agen terapi kanker payudara juga menimbulkan efek samping metastasis. Daun awar-awar (Ficus septica Burm. F.) mengandung senyawa golongan alkaloid fenantroindolisidin. Ekstrak daun awar-awar dan alkaloid fenantroindolisidinnya berefek sitotoksik pada sel kanker payudara dan sinergis terhadap agen kemoterapi. Artikel ini mengulas potensi daun awar-awar sebagai agen pendamping kemoterapi (ko-kemoterapi), khususnya melalui mekanisme antimetastasis. Aktivitas sitotoksik daun awar-awar pada sel kanker terjadi melalui peningkatan apoptosis dan modulasi siklus sel. Daun awar-awar menghambat metastasis sel kanker payudara dengan menurunkan ekspresi VEGF, MMP-9, dan Rac-1 yang terlibat dalam metastasis. Oleh karena itu, dapat disimpulkan bahwa daun awar-awar berpotensi untuk dikembangkan sebagai agen ko-kemoterapi antimetastasis untuk kanker payudara. 
Pengaruh Perbandingan Tween 80 dan Fosfatidilkolin Pada Formulasi Transfersom Naringenin dan Kajian Permeasi Berbasis Hidrogel
Authors: Kuncahyo Ilham , Resmi Juniar Kalpika , Muchalal M
Published: November 2021

Naringenin merupakan flavonoid isolat kulit buah jeruk yang mempunyai kelarutan yang rendah. Potensi naringenin dalam terapi dermatitis atopik pada sistem penghantaran transfersom dapat meningkatkan kemampuan naringenin dalam berpenetrasi ke dalam kulit sehingga mampu mengefektifkan proses penyembuhan. Penelitian ini bertujuan untuk mengkaji pengaruh perbandingan molar fosfatidilkolin sebagai pembentuk vesikel dan Tween 80 yang berfungsi sebagai surfaktan terhadap karakteristik transfersom naringenin dan mengetahui permeasi narigenin terinkorporasi dalam trasfersom berbasis hidrogel. Formula dibuat menjadi 4 untuk diaplikasikan pada proses pembuatan transfersom naringenin dengan variasi perbandingan molar fosfatidilkolin dan Tween 80 yaitu 97,5:2,5 (FI), 90:10 (FII), 85:15 (FIII), dan 80:20 (FIV) menggunakan teknik evaporation-ultrasonication. Hasil transfersom naringenin masing-masing formula dilakukan karakterisasi terhadap ukuran partikel, indeks polidispersi, dan efisiensi penjerapan. Formula transfersom terpilih diformulasikan dalam sediaan gel dan dilakukan uji permease menggunakan membran selofan (WMCO 8 kDa). Hasil penelitian menunjukkan bahwa peningkatan jumlah surfaktan akan menurunkan ukuran partikel dan peningkatan jumlah fosfatidilkolin akan meningkatkan jumlah obat yang terjerap. Formula dengan perbandingan Phospholipon 90G: Tween 80 (85:15, F3) merupakan formula terpilih dengan ukuran dan indeks polidispersi partikel masing-masing 115,6 nm dan 0,274 serta efisiensi penjerapan sebesar 88,63 ± 0,59%. Sediaan gel naringenin transfersom memberikan nilai jumlah kumulatif dan laju penetrasi yang besar dibandingkan gel naringenin tanpa transfersom.
Analisis Hubungan Pengetahuan Terhadap Kepatuhan Terapi Pada Pasien Hipertensi di Puskesmas Pucang Sawit Surakarta
Authors: Farida Yeni , Salsabila Yumna Zulfa , Amsari Alfiani , Niruri Rasmaya , Yugatama Adi , Handayani Nestri , Prihapsara Fea
Published: November 2021

Hipertensi yang tidak terkendali dengan baik dapat meningkatkan terjadinya komplikasi, untuk itu diperlukan kepatuhan pasien dalam terapi hipertensi. Kepatuhan dipengaruhi oleh beberapa faktor, salah satu faktornya adalah pengetahuan. Tujuan penelitian adalah untuk mengetahui hubungan pengetahuan terhadap kepatuhan terapi pasien hipertensi di Puskesmas Pucangsawit Surakarta. Studi crosssectional dilakukan pada populasi pasien hipertensi di Puskesmas Pucang Sawit Surakarta yang memenuhi kriteria inklusi. Sampel ditetapkan dengan kriteria pasien mendapatkan terapi hipertensi minimal 1 bulan, melakukan kunjungan kontrol ke Puskesmas di bulan Maret 2019, serta bersedia menjadi responden yang dibuktikan dengan informed consent. Pengetahuan dinilai dengan kuesioner yang disusun peneliti berdasarkan panduan pharmaceutical care untuk penyakit hipertensi, sedangkan kepatuhan dinilai dengan kuesioner hill-bone. Pengetahuan dikategorikan menjadi baik, cukup dan kurang, sedangkan kepatuhan dikategorikan menjadi patuh dan tidak patuh. Analisis variabel dilakukan dengan uji Pearson correlation. Hasil penelitian menunjukkan bahwa 5,3% responden memiliki pengetahuan yang baik, 27,1% berpengetahuan cukup dan 17,6% berpengetahuan kurang. Tidak terdapat hubungan yang signifikan antara pengetahuan dengan kepatuhan terapi hipertensi pada pasien di Puskesmas Pucang Sawit Surakarta, sedangkan arah korelasinya positif tetapi korelasi antara keduanya sangat lemah (p = 0,319; r = 0,109). 
Membrane-Inducing “Masquelet” Technique for Massive Traumatic Diaphyseal Femur Bone Loss in a Pediatric Patient
Authors: Cardin Stefano , Garrett Christopher H. , Williams Warren A. , Langford Joshua R. , Herrera-Soto José A.
Published: November 2021

Case:

A 14-year-old boy sustained 22 cm of femur bone loss after a motor vehicle accident. The patient underwent treatment with the membrane-inducing “Masquelet” technique for management of the injury. The grafts incorporated to form new bone and fill-in the void.

Conclusion:

Although the Masquelet technique is thoroughly described in adult orthopaedic trauma and oncology literature, there are minimal reports to support its use in pediatric patients. Five-year follow-up data on this patient concluded that utilization of this technique for significant bone defects proves to be a safe and effective alternative for the management of pediatric trauma patients.
Tuberculosis of the Cuboid in a 16-Month-Old Child
Authors: Patwardhan Sandeep , Sodhai Vivek , Sable Aashiket , Shyam Ashok , Sancheti Parag
Published: November 2021

Case:

A 16-month-old male child presented with swelling over the dorsolateral aspect of the right foot associated with limp and no other constitutional symptoms. Tenderness and swelling were noted over the dorsolateral aspect, and radiographs revealed an eccentric lytic expansile lesion in the right cuboid. Biopsy of the lesion revealed necrotizing granulomas, and molecular testing later documented tuberculosis. The child received antitubercular medications and was relieved of symptoms in 6 weeks. Complete healing was observed after 12 months.

Conclusion:

In developing countries, a high index of suspicion helps in the early diagnosis and appropriate management of tubercular osteomyelitis in children.
An Unusual Presentation of Osteogenesis Imperfecta
Authors: Lindsay Sarah E. , Nicol Lindsey E. , Gamayo Ashley C. , Raney Ellen M.
Published: November 2021

Case:

We report an 18-year-old patient with a clinical phenotype consistent with severe osteogenesis imperfecta (OI) with frequent fractures, short stature, shortening and bowing of extremities, and unusual radiographic features of severe fibrous dysplasia, including lytic lesions and a “ground-glass” appearance. Genetic testing for the patient was notable for a c.119C>T (p.Ser40Leu) variant in exon 1 of IFITM5 and a c.676C>A (Pro226Thr) variant in exon 5 of CREB3L1.

Conclusion:

This unusual skeletal presentation was in the setting of a rare IFITM5 mutation and represents a unique case of severe OI.
Artificial ground freezing using solar-powered thermosyphons
Authors: Wagner Anna , Maakestad Jon , Yarmak Edward , Douglas Thomas
Published: November 2021

Thermosyphons are an artificial ground-freezing technique that has been used to stabilize permafrost since the 1960s. The largest engineered structure that uses thermosyphons to maintain frozen ground is the Trans Alaska Pipeline, and it has over 124,000 thermosyphons along its approximately 1300 km route. In passive mode, thermosyphons extract heat from the soil and transfer it to the environment when the air temperature is colder than the ground temperature. This passive technology can promote ground cooling during cold winter months. To address the growing need for maintaining frozen ground as air temperatures increase, we investigated a solar-powered refrigeration unit that could operate a thermosyphon (nonpassive) during temperatures above freezing. Our tests showed that energy generated from the solar array can operate the refrigeration unit and activate the hybrid thermosyphon to artificially cool the soil when air temperatures are above freezing. This technology can be used to expand the application of thermosyphon technology to freeze ground or maintain permafrost, particularly in locations with limited access to line power.
Methodology for the analysis of geospatial and vehicle datasets in the R language
Authors: Fuentes Anthony , Michaels Michelle , Shoop Sally
Published: November 2021

The challenge of autonomous off-road operations necessitates a robust understanding of the relationships between remotely sensed terrain data and vehicle performance. The implementation of statistical analyses on large geospatial datasets often requires the transition between multiple software packages that may not be open-source. The lack of a single, modular, and open-source analysis environment can reduce the speed and reliability of an analysis due to an increased number of processing steps. Here we present the capabilities of a workflow, developed in R, to perform a series of spatial and statistical analyses on vehicle and terrain datasets to quantify the relationship between sensor data and vehicle performance in winter conditions. We implemented the R-based workflow on datasets from a large, coordinated field campaign aimed at quantifying the response of military vehicles on snow-covered terrains. This script greatly reduces processing times of these datasets by combining the GIS, data-assimilation and statistical analyses steps into one efficient and modular interface.
Bioorthogonal labeling of live dissociated and organotypic slice culture neurons
Authors: Bessa-Neto Diogo , Pecoraro Valeria , Beliu Gerti , Choquet Daniel
Published: November 2021

Over the past couple decades, the explosion in the development of high-resolution and super-resolution microscopy techniques has led to the need for the development of new protein labeling techniques. Click-labeling via genetic code expansion (GCE) has received particular attention given its potential has the ultimately small labelling probe for proteins. Click-labeling via GCE offers a reliable and sterically minimally demanding capacity to label proteins, but its application in non dividing cells such as neurons remains poorly exploited due to its low efficiency. Here, we describe a simple, efficient and reproducible protocol that allows to fluorescently label transmembrane proteins in live neurons using click-labeling via GCE, both in dissociated culture and organotypic brain slices.
Habitat formed by the invasive macroalga Caulerpa filiformis (Suhr) Hering (Caulerpales, Chlorophyta) alters benthic macroinvertebrate assemblages in Peru
Authors: Aguilar Silvia , Moore Pippa J. , Uribe Roberto A.
Published: November 2021

The green macroalga Caulerpa filiformis has been spreading on shallow soft sediment habitats along the Peruvian coast, colonizing previously unvegetated sediments to create monospecific meadows. We examined the nature of the impact of C. filiformis meadows on the density, taxonomic richness and assemblage structure of epifaunal and infaunal benthic macroinvertebrates. Specifically, we tested whether the spread of C. filiformis has resulted in different macroinvertebrate assemblages than those formed by the dominant native macroalgae (i.e., Rhodymenia spp.) and unvegetated sediments. Surveys were undertaken in two bays in each of two locations, in central and southern Peru, during winter 2017 and summer 2018. In general, our results show that macroinvertebrate assemblages were similar across all three habitats, although there were some differences, related to location and season, but with no clear patterns observed. Taxonomic richness and density was generally higher in the vegetated habitats than the unvegetated habitat, and where there were differences between the two vegetated habitats there was no consistent pattern of which habitat supported the highest richness or density. Given invading C. filiformis is primarily colonizing unvegetated habitats it would appear that this species is creating a new niche which supports similar assemblages, but higher taxonomic richness and density than unvegetated habitats. While our study suggests that C. filiformis is having a limited ecological impact we recommend that actions be put in place to limit the spread of this invasive species at the same time as increasing monitoring of the ecological impacts of this species as lags in the ecological impacts of invasive species are common.
Unnecessary Chest Tube for a Hemodialytic Patient with Multiple Trauma: A Case Report
Authors: Reisi-Vanani Vahid , Esfahani Hooman
Published: November 2021

Background

Pneumothorax (PTX) is a life-threatening condition that overdiagnosis could result in increases in mortality and morbidity of patients, this overdiagnosis would be increased if physicians do not manage the patient classically and do not pay attention to the physical exam and history of the patient.
Case presentation:

A-71-year old man was admitted to the emergency department due to multiple trauma. His vital signs were stable and in examination, there were two lacerations on his scalp with venous bleeding source and galea transaction; there were also some abrasions all over his body including his thorax. In the physical exam, there was no sucking lesion, decreases in respiratory sounds in auscultation or chest deformity but he had little right hemithorax rib tenderness. In more evaluations, there was a suspected visceral line of pleura in his CXR and no plural sliding movement was seen in E-FAST by the operator. Due to the inconsistency in physical exam and radiologic findings we decided to take a chest CT-scan before the insertion of the chest tube that indicated no PTX for him and the suspected visceral line in CXR was skin fold of a permacath for hemodialysis.
Conclusions

Several conditions could mimic findings of PTX in CXR that every physician should know and pay attention to them besides special attention to the history taking and physical examination to reduce the mortality and morbidity of patients.
Recombinantly Expressed MeICT, a new Toxin from Mesobuthus Eupeus Scorpion, Inhibits Glioma Cell Proliferation and Downregulates Annexin A2 and FOXM1 Genes
Authors: Ayat Hoda , Gandomkari Maryam Shahbazi , Ahadi Ali Mohammad
Published: November 2021

Gliomas are highly invasive and lethal malignancy that do not respond to current therapeutic approaches. Novel therapeutic agents are required to target molecular mechanisms involved in glioma progression. MeICT is a new short-chain toxin isolated from Mesobuthus eupeus scorpion venom. This toxin contained 34 amino acid residues and belongs to chloride channels toxins. In this study, the coding sequence of MeICT was cloned into the pET32Rh vector and a high yield of soluble recombinant MeICT was expressed and purified. Recombinant MeICT-His significantly inhibited the proliferation and migration of glioma cells at low concentration. In vivo studies showed that MeICT was not toxic when administrated to mice at high doses. We also determined the effect of MeICT on the mRNA expression of MMP-2, Annexin A2 and FOXM-2 that are key molecules in the progression and invasion of glioma. Expression of Annexin A2 and FOXM1 mRNA was significantly down-regulated following treatment with MeICT. However, no significant decrease in the expression of MMP-2 gene was identified. In this study a short toxin with four disulfide bonds was successfully produced and its anti-cancer effects was detected. Our findings suggest that recombinant MeICT can be considered as a new potent agent for glioma targeting.
Immunoglobulin (IgG4) Sclerosing Cholecystitis- Camouflaging Gall Bladder Cancer- A Case Report and Review of Literature
Authors: Singh Rashpal , Mahajan Puneet , Prasher Rizul , Rajdev Vivek , Singh Jagwinder , Kattoor Cliffin Mathai , Mardi Kavita , Gupta Jagdish
Published: November 2021

Background: IgG4 related disease is a rare systemic disorder having an underlying autoimmune cause. These disorders mainly affects pancreatico biliary tree i.e. pancreas, gall bladder and biliary tree (extrahepatic as well intrahepatic),but can also affect other part of body.Majority of disorders involving biliary tree are associated with autoimmune pancreatitis component.These disorders are difficult to diagnose clinically as they can mimic inflammatory as well malignancy and poses a real diagnostic challenge to manage and treat.Case presentation: 64 years old female known diabetic evaluated for pain in right hypochondrium. Gall bladder cancer was suspected clinically as well on radiologically basis. Patient underwent extended cholecystectomy as it was a resectable disease.Final histopathology revealed immunoglobulin G4 (IgG4) related cholecystitis which was confirmed after immunohistochemistry for CD 138 and IgG4.This disease could be managed conservatively by giving oral steroids ,if it has been picked up preoperatively and major surgical intervention have been avoided. No defined blood test or tumour markers are currently available to diagnose this entity except serum immunoglobulin G4 which is costly and not feasible to get done in all patients especially in developing nations like India.Conclusion: IgG4 cholecystitis is an immune mediated disease whose pathophysiology is still not completely understood. Every clinician should keep possibility of IgG4 cholecystitis in mind whenever any patient with abnormal gall bladder thickening or gall bladder mass is encountered in their clinical practice, as both these entities have completely different options of treatment. We should not rely solely upon clinical and radiological picture.
Effects of MRI on Stemness Properties of Wharton’s Jelly Derived Mesenchymal Stem Cells
Authors: Tashakori Mahnaz , Asadi Fatemeh , Khorram Faezeh-Sadat , Manshoori Azita , Hosseini-Chegeni Ali , Kamalabadi Mahdieh Ahmadi , Yousefi-Ahmadipour Aliakbar
Published: November 2021

BackgroundMesenchymal stem cells (MSCs), derived from various tissues, have served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton’s jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. Material and methodsThe aim of this study was to evaluate the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. In addition, we assessed cell viability, growth kinetics and apoptosis of WJ-MSCs after MRI treatment. ResultsThe quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) data showed that transcript levels of SOX-2, NANOG in MRI-treated WJ-MSCs were increased 32- and 213-fold, respectively. MTT assay was performed at 24, 48, and 72 hours post-treatment and the viability was not significantly difference between two groups. The doubling time of MRI group was markedly higher than control group. In addition, the colony formation ability of WJ-MSCs after MRI treatment significantly increased. Furthermore, no change in apoptosis was seen before or after MRI treatment. ConclusionsOur results suggest the use of MRI can improve quality of MSCs and may enhance the efficacy of mesenchymal stem cell-based therapies.
Peri-Operative Medication Errors in a Tertiary Care Teaching Hospital of a Low-Middle Income Country
Authors: Abbasi Shemila , Rashid Saima , Khan Fauzia Anis
Published: November 2021

BackgroundIdentifying medication errors is one method of improving patient safety. Peri operative anesthetic management of patient includes polypharmacy and various steps prior to drug administration. Our objective was to analyze the medication errors reported in our critical incident reporting system (CIRS) database over the last 15 years (2004-2018) and to review measures taken for improvement based on the reported errors.MethodsAll Critical incidents (CI) reported during January 2004 till December 2018 were retrieved from CIRS database. Medication errors were identified and entered on a data extraction form which included reporting year, patients age, surgical specialty, ASA status, time of incident, phase and type of anesthesia and drug handling, type of error, class of medicine, level of harm, severity of adverse drug event (ADE) and steps taken for improvement.Results 311 medication errors were reported. Fifty two percent errors occurred in ASA II and III patient, and 43% during induction. Sixty % occurred during administration phase and 65 % were due to human error. Thirty seven percent were ADE, 58 of which were significant, 23 serious and five life-threatening errors. Majority errors involved neuromuscular blockers (32%) and opioids (13%).Conclusion Sharing of CI and a lesson to be learnt e-mail, colour coded labels, change in medication trolley lay out, decrease in floor stock and high alert labels were the low-cost steps taken to reduce incidents.Medication errors were more frequent during administration. Twenty eight percent resulted in significant, serious, or life-threatening events.
The Efficacy and Safety of Postoperative Tranexamic Acid, Hemocoagulase Agkistrodon, and their Combination in Patients Undergoing Heart Valve Replacement With Cardiopulmonary Bypass
Authors: Chen Jingxiu , Li Jingjie , Yan Jiajia , He Qiuyi , Huang Min , Chen Xiao , Chen Pan
Published: November 2021

Background

Excessive bleeding is a major complication in patients undergoing cardiac surgery. We aimed to compare the efficacy and safety of postoperative tranexamic acid (TXA), hemocoagulase agkistrodon and their combination in patients undergoing heart valve replacement surgery with cardiopulmonary bypass (CPB).
Methods

This was a retrospective study. The enrolled patients were intravenously injected with TXA at a dose of 1.0 g during the intraoperative period. After surgery, the patients were assigned to four groups: the control group (Group C), the TXA group (Group T), the hemocoagulase agkistrodon group (Group H) and the combination group (Group TH). The primary efficacy outcomes were the total blood loss (TBL) from the time of the operation to postoperative Day 2, postoperative blood loss within 2 days, and transfusion of red blood cells and plasma from the operation to postoperative Day 3. The primary safety endpoint was the incidence of thromboembolic events.
Results

A total of 252 patients were recruited. There were no statistically significant differences in terms of the TBL, postoperative blood loss, volumes of red blood cells or plasma transfusion among the four groups. However, an increased total pericardial drainage volume and longer length of stay in the ICU were found in Group H compared with in Group T. In addition, increased volumes of total pericardial drainage were found in Group TH compared with Groups C and T. A similar result was also found in the number of days of pericardial drainage. Regarding safety outcomes, fibrinogen levels on postoperative Days 1 and 2 in Groups H and TH were significantly lower than those in Groups C and T, while the frequencies of human fibrinogen transfusion in Groups H and TH were higher, with the highest frequency in Group H. The transfusions of human fibrinogen among Groups C, T, H and TH were 1.45%, 2.78%, 64.71%, and 28.72%, respectively. No significant differences were found in the postoperative incidences of thromboembolic events and acute kidney injuries among all groups.
Conclusions

Bleeding events after cardiac valve replacement surgery with CPB were not improved by postoperative administration of TXA, hemocoagulase agkistrodon or their combination. Hemocoagulase agkistrodon is related to hypofibrinogenemia and increased transfusions of human fibrinogen.
A New Method of Identifying Key Industries: A Principal Component Analysis
Authors: Tsoulfidis Lefteris , Athanasiadis Ioannis
Published: November 2021

This article using the principal components analysis identifies key industries and groups them into particular clusters. The data come from the US benchmark input-output tables of the years 2002, 2007, 2012 and the most recently published input-output table of the year 2019. We observe some intertemporal switches of industries both between and within the top clusters. The findings further suggest that structural change is a slow moving process and it takes time for some industries to move from one cluster to the other. This information may be proved important in the designation of effective economic policies by targeting particular industries and also for the stability properties of the economic system.JEL classifications: B24, B51, C67, D46, D57, E11, E32
Assessing the Determinants of Electricity Generation Infrastructure in Nigeria
Authors: Olanrele Iyabo
Published: November 2021

The supply and demand for electricity have outpaced available infrastructure in Nigeria despite the abundant energy resources. The paper investigates the determinants of electricity generating infrastructure in Nigeria for the period 1980 to 2016. Using an Autoregressive Distributed Lag model, electricity generation capacity was used as an indicator for electricity infrastructure development. Its expansion was based on the behaviour of inflation rate, total government expenditure, interest rate, private sector financial credit, exchange rate, real GDP per capita, real gross fixed capital formation, and the rate of urbanisation. Financial credit to private sector, total public expenditure, real per capita income, real gross fixed capital formation, urbanization, and exchange rate adversely affect the development of electricity generation capacity. Investment in generating assets is capital intensive, which should be matched with adequate private sector financing. If the power sector subsidy will remain and achieve its objective, strategies that will lead to sustaining exchange rate stability should be promoted. Based on estimate, every one million population require 1000MW of electricity to function in modern-day society implying that Nigeria needs 180,000MW of electricity capacity. The realisation of this is hinged on large scale electricity infrastructure investment enabled, partly, by the favourable macroeconomic environment.JEL Classification: E16, O1, O2
Novel Mutation of SIK1 Gene Causing a Mild Form of Pediatric Epilepsy in a Chinese Family
Authors: Xu Wangshu , Zhang Wenqun , Cui Lili , Shi Lei , Zhu Bin , lv Tianjie , Ma Wenping
Published: November 2021

Objective: Early infantile epileptic encephalopathy (EIEE) is a group of disorders affecting children at early stages of infancy, which is characterized by frequent seizures, epileptiform activity on EEG, and developmental retardation or regression. Salt-inducible kinases (SIKs) syndrome is a newly described EIEE, caused by heterozygous mutations in the salt-inducible kinase SIK1, which can present as early myoclonic encephalopathy, Ohtahara syndrome, and infantile spasms. Methods: In this study, we investigated a patient with early onset epilepsy. DNA sequencing of the whole coding region revealed a de novel heterozygous nucleotide substitution (c.880G>A) causing a missense mutation (p.A294T). This mutation was classified as variant of unknown significance (VUS) by American College of Medical Genetics and Genomics (ACMG). To further investigate the pathogenicity and pathogenesis of this mutation, we established a human neuroblastoma cell line (SH-SY5Y) stably-expressing wild type SIK1 and A294T mutant, and compared the transcriptome and metabolomics profiles. Results: We presented a pediatric patient suffering from infantile onset epilepsy. Early EEG showed a boundary dysfunction of activity and MRI scan of the brain was normal. The patient responded well to single anti-epileptic drug treatment. Whole-exome sequencing found a missense mutation of SIK1 gene (c.880G>A chr21: 43420326 p. A294T). Dysregulated transcriptome and metabolome in cell models expressing WT and MUT SIK1 confirmed the pathogenicity of the mutation. Specifically, we found MEF2C target genes, certain epilepsy causing genes and metabolites are dysregulated by SIK1 mutation.We found MEF2C target genes, certain epilepsy causing genes and metabolites are dysregulated by SIK1 mutation. Significance: Our finding further expanded the disease spectrum and provided novel mechanistic insights of SIK1 syndrome.
Consumption of Coconut Oil Affects adipose miRNA Profile in Pigs.
Authors: Oczkowicz Maria , Pawlina-Tyszko Klaudia , Świątkiewicz Małgorzata , Szmatoła Tomasz
Published: November 2021

Circulating miRNA molecules are intensively studied for their usefulness as biomarkers of civilization diseases. At the same time, it is known that diet can influence the level of miRNA expression in tissues. Our research aimed to determine how a diet containing various sources of fat (rapeseed oil, beef tallow, coconut oil) and different amounts of cDDGS (corn Dried Distilled Grains with Solubles) affects the miRNA profile in pig fat – the main source of circulating miRNAs. For this purpose, we used Next Generation Sequencing of miRNA libraries. We observed the highest number of differentially expressed miRNAs in the samples from animals that were fed with coconut oil in the diet compared to all other treatments. In contrary, cDDGS appeared to have little effect on miRNA expression. We propose a subset of di-et-related, adipose-specific, conservative miRNAs among mammals, namely: ssc-miR-99b, ssc-miR-4334-3p, ssc-miR-146b, ssc-miR-23a. Moreover, we observed that several miRNAs regulated by dietary fats are considered as biomarkers in human and animal diseases.
Incidence and Effects of Posterior Cranial Vault Deformation in Adult Females With Skeletal Class III Malocclusion
Authors: Fujimoto Kouta , Sasaki Au , Kawajiri Akemi , Yamaguchi Koji , Oshima Takafumi , Togane Yuri , Otsuka Yuichiro , Suda Naoto
Published: November 2021

BackgroundThe precise characteristics of posterior cranial vault deformation are difficult to identify using a two-dimensional analysis. Recently, three-dimensional analysis using computed tomography (CT) data and software analysis has enabled us to examine the craniofacial structure precisely. To identify the deformation in the cranial vaults by a three-dimensional analysis and examine its effects on other structures, CT data of skeletal mandibular protrusion cases without lateral deviation of pogonion (Pog) were examined.MethodsThirty-two adult female patients with skeletal mandibular protrusion who visited Meikai University Hospital were included in this study. CT images were taken before orthognathic surgeries, and the craniofacial structures were examined threedimensionally.The lateral deviation of Pog was less than 3 mm in all cases.Genetic/congenital anomaly cases, endocrine disorders, or cases with severe trauma or temporomandibular disorder were excluded.ResultsMany cases had three-dimensional deformations in the cranial vaults. The cranial vaults with unilateral posterior deformation had posteriorly positioned mandibular fossae on the same sides. Mandibular body lengths were related to the position of the mandibular fossae, and the posterior mandibular fossae tended to have longer mandibular body lengths on the same sides. Masseter muscles with larger volumes were related to longer mandibular body lengths.Conclusions﻿Mandibular body lengths can compensate for the bilateral difference in the position of the mandibular fossae to avoid lateral deviation of the Pog. The masseter muscles are likely one of the factors related to the bilateral differences in the mandibular body length.