bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Speed Machining of Malleable Cast Iron by Various Cutting Tools Coated by Physical Vapor Deposition
Authors: Li Suyang , Lin Haisheng , Zhang Tingjie , Sui Jianbo , Wang Chengyong
Published: May 2021

The coating material of a tool directly affects the efficiency and cost of machining malleable cast iron. However, the machining adaptability of various coating materials to malleable cast iron has been insufficiently researched. In this paper, turning tests were conducted on cemented carbide tools with different coatings (a thick TiN/TiAlN coating, a thin TiN/TiAlN coating, and a nanocomposite (nc) TiAlSiN coating). All coatings were applied by physical vapor deposition. In a comparative study of chip morphology, cutting force, cutting temperature, specific cutting energy, tool wear, and surface roughness, this study analyzed the cutting characteristics of the tools coated with various materials, and established the relationship between the cutting parameters and machining objectives. The results showed that in malleable cast iron machining, the coating material significantly affects the cutting performance of the tool. Among the three tools, the nc-TiAlSiN-coated carbide tool achieved the minimum cutting force, the lowest cutting temperature, least tool wear, longest tool life, and best surface quality. Moreover, in comparisons between cemented-carbide and compacted-graphite cast iron machined under the same conditions, the wear mechanism of the coated tools was found to depend on the cast iron being machined. Therefore, the performance requirements of a tool depend on multiple factors, and selecting an appropriately coated tool for a particular cast iron material is essential.
Post-traumatic trigeminal neuropathy: correlation between objective and subjective assessments and a prediction model for neurosensory recovery
Authors: Meewis Jeroen , Renton Tara , Jacobs Reinhilde , Politis Constantinus , Van der Cruyssen Fréderic
Published: May 2021

Background

Post-traumatic trigeminal neuropathy (PTN) can have a substantial effect on patient well-being. However, the relation between the neuropathic symptoms and their effect on psychosocial functioning remains a matter of debate. The purpose of this study was to evaluate the association between objective and subjective assessments of neurosensory function in PTN and predict neurosensory outcome using baseline measurements.

Methods

This prospective observational cohort study included patients diagnosed with PTN at the Department of Oral and Maxillofacial Surgery, University Hospital Leuven, Belgium, between April 2018 and May 2020. Standardized objective and subjective neurosensory examinations were recorded simultaneously on multiple occasions during the follow-up period. Correlation analyses and principal component analysis were conducted, and a prediction model of neurosensory recovery was developed.

Results

Quality of life correlated significantly (P < 0.05) with percentage of affected dermatome (ρ = − 0.35), the presence of brush stroke allodynia (ρ = − 0.24), gain-of-function sensory phenotype (ρ = − 0.41), Medical Research Council Scale (ρ = 0.36), and Sunderland classification (ρ = − 0.21). Quality of life was not significantly correlated (P > 0.05) with directional discrimination, stimulus localization, two-point discrimination, or sensory loss-of-function. The prediction model showed a negative predictive value for neurosensory recovery after 6 months of 87%.

Conclusions

We found a strong correlation of subjective well-being with the presence of brush stroke allodynia, thermal and/or mechanical hyperesthesia, and the size of the neuropathic area. These results suggest that positive symptoms dominate the effect on affect. In patients reporting poor subjective well-being in the absence of positive symptoms or a large neuropathic area, additional attention towards psychosocial triggers might enhance treatment outcome. The prediction model could contribute to establishing realistic expectations about the likelihood of neurosensory recovery but remains to be validated in future studies.
Genome-wide (ChIP-seq) identification of target genes regulated by WRKY33 during submergence stress in Arabidopsis
Authors: Zhang Junlin , Liu Bao , Song Yan , Chen Yang , Fu Jiao , Liu Jianquan , Ma Tao , Xi Zhenxiang , Liu Huanhuan
Published: May 2021

Background

Hypoxia induced by flooding causes significant losses to crop production almost every year. However, the molecular network of submergence signaling pathway is still poorly understood. According to previous studies, transgenic plants overexpressing the WRKY33 gene showed enhanced resistance to submergence stress. Thus, this transcription factor may regulate a series of target genes in response to submergence. Here, to determine putative downstream targets of WRKY33 at a genome-wide scale in Arabidopsis thaliana, we performed the chromatin immunoprecipitation sequencing (ChIP-seq) using 35S:FLAG-WRKY33 overexpression transgenic lines (WRKY33-OE) after 24 h of submergence treatment.

Results

Using ChIP-seq data, we identified a total of 104 WRKY33-binding genes under submergence stress (WRKY33BGSs). Most WRKY33BGSs are involved in the oxidation-reduction process, programmed cell death in response to reactive oxygen species, lipid biosynthesis process, and other processes related to stress responses. Moreover, the major motif identified in the WRKY33BGSs promoters is a new cis-element, TCTCTC (named here as “TC box”). This cis-element differs from the previously known W box for WRKY33. Further qPCR experiments verified that genes carrying this motif in their promoters could be regulated by WRKY33 upon submergence treatment.

Conclusions

Our study has identified a new putative binding motif of WRKY33 and recovered numerous previously unknown target genes of WRKY33 during submergence stress. The WRKY33 gene positively participates in flooding response probably by transcriptional regulation of the downstream submergence-related target genes via a “TC box”.
Transcriptomic and metabolomic characterization of post-hatch metabolic reprogramming during hepatic development in the chicken
Authors: Van Every Heidi A. , Schmidt Carl J.
Published: May 2021

Background

Artificial selection of modern meat-producing chickens (broilers) for production characteristics has led to dramatic changes in phenotype, yet the impact of this selection on metabolic and molecular mechanisms is poorly understood. The first 3 weeks post-hatch represent a critical period of adjustment, during which the yolk lipid is depleted and the bird transitions to reliance on a carbohydrate-rich diet. As the liver is the major organ involved in macronutrient metabolism and nutrient allocatytion, a combined transcriptomics and metabolomics approach has been used to evaluate hepatic metabolic reprogramming between Day 4 (D4) and Day 20 (D20) post-hatch.

Results

Many transcripts and metabolites involved in metabolic pathways differed in their abundance between D4 and D20, representing different stages of metabolism that are enhanced or diminished. For example, at D20 the first stage of glycolysis that utilizes ATP to store or release glucose is enhanced, while at D4, the ATP-generating phase is enhanced to provide energy for rapid cellular proliferation at this time point. This work has also identified several metabolites, including citrate, phosphoenolpyruvate, and glycerol, that appear to play pivotal roles in this reprogramming.

Conclusions

At Day 4, metabolic flexibility allows for efficiency to meet the demands of rapid liver growth under oxygen-limiting conditions. At Day 20, the liver’s metabolism has shifted to process a carbohydrate-rich diet that supports the rapid overall growth of the modern broiler. Characterizing these metabolic changes associated with normal post-hatch hepatic development has generated testable hypotheses about the involvement of specific genes and metabolites, clarified the importance of hypoxia to rapid organ growth, and contributed to our understanding of the molecular changes affected by decades of artificial selection.
Identification of new demands regarding prehospital care based on 35,188 missions in 2018
Authors: Vuilleumier Séverine , Fiorentino Assunta , Dénéréaz Sandrine , Spichiger Thierry
Published: May 2021

Background

Population ageing and increased prevalence of chronic diseases result in the emergence of new demands in prehospital care. The prehospital system is facing an increase of cases without acute threat to life (so-called “non-urgent”), which generates tension due to a higher number of admissions to emergency departments and a greater use of prehospital resources. Our aim is to understand this transition in prehospital activities and to delineate the primary missions performed by paramedics in 2018 with a focus on the population concerned, the severity of cases encountered and the typology of health issues.

Method

The study is retrospective, and descriptive, using a statistical description of 35,188 primary missions realized in 2018 in the State of Vaud (Switzerland). The characteristics taken into consideration are the age and gender of patients, as well as the health issue, the severity of cases based on National Advisory Committee for Aeronautics score (NACA score), and the time and place of intervention.

Results

The results describe the primary missions in the State of Vaud in 2018 and show that 87% of missions concern “non-urgent” situations (without acute threat to life). Over half of patients are 65 or older, the highest proportion of health issues, 49%, are medical and only 23% of missions are for traumas. Mission related to mental health issues reach 7% and those for intoxication 6%. Most missions take place between 7:00 am and 6:00 pm (67%), and around 12% of missions lead to the non-transport of the patient.

Conclusion

The prehospital sector is confronted with a major transition in terms of patient care. An increase of non-urgent cases is observed, associated with the care of persons aged 65 or more. Our results question the adequacy between the needs in terms of prehospital care and the paramedic profession as it is currently defined, as well as the place of this profession within the health network. Reflecting upon the role of paramedics with respect to the socio-demographic evolution of populations appears necessary, to analyse the adequacy of the paramedics’ skills to respond to the current needs.
Soluble ST2 and risk of cognitive impairment after acute ischemic stroke: a prospective observational study
Authors: Zhu Yinwei , Fang Chongquan , Zhang Qi , Lu Yaling , Zhang Rui , Wang Aili , Bu Xiaoqing , Zhang Jintao , Ju Zhong , Zhang Yonghong , Xu Tan , Zhong Chongke
Published: May 2021

Background

Soluble suppression of tumorigenesis-2 (sST2) was reported to be associated with cognitive performance and risk of incident stroke. However, the impact of sST2 on cognitive function after ischemic stroke is unclear. We aimed to assess the association of sST2 and cognitive impairment at 3 months in acute ischemic stroke patients.

Methods

Baseline plasma sST2 levels were measured in 619 ischemic stroke patients (mean age: 60.0 ± 10.5 years) from 7 participating hospitals of the China Antihypertensive Trial in Acute Ischemic Stroke. Montreal Cognitive Assessment (MoCA) and Mini-Mental State Examination (MMSE) were used to assess cognitive status. Cognitive impairment was defined as a MoCA score < 23 or MMSE score < 27. The association between sST2 and cognitive impairment was evaluated by logistic regression analysis.

Results

325 (52.5%) or 323 (52.2%) participants developed cognitive impairment according to MoCA or MMSE. After adjustment for age, sex, education, and other covariates, the odds ratio for the highest vs lowest quartile of sST2 was 2.38 (95% CI, 1.42–4.00) and 1.82 (95% CI 1.09–3.03) risk of cognitive impairment defined by MoCA and MMSE score, respectively. Incorporation sST2 into a model containing conventional risk factors significantly improved reclassification.

Conclusions

Elevated plasma sST2 levels were significantly associated with post-stroke cognitive impairment.
Development of a bedside score to predict dengue severity
Authors: Marois Ingrid , Forfait Carole , Inizan Catherine , Klement-Frutos Elise , Valiame Anabelle , Aubert Daina , Gourinat Ann-Claire , Laumond Sylvie , Barsac Emilie , Grangeon Jean-Paul , Cazorla Cécile , Merlet Audrey , Tarantola Arnaud , Dupont-Rouzeyrol Myrielle , Descloux Elodie
Published: May 2021

Background

In 2017, New Caledonia experienced an outbreak of severe dengue causing high hospital burden (4379 cases, 416 hospital admissions, 15 deaths). We decided to build a local operational model predictive of dengue severity, which was needed to ease the healthcare circuit.

Methods

We retrospectively analyzed clinical and biological parameters associated with severe dengue in the cohort of patients hospitalized at the Territorial Hospital between January and July 2017 with confirmed dengue, in order to elaborate a comprehensive patient’s score. Patients were compared in univariate and multivariate analyses. Predictive models for severity were built using a descending step-wise method.

Results

Out of 383 included patients, 130 (34%) developed severe dengue and 13 (3.4%) died. Major risk factors identified in univariate analysis were: age, comorbidities, presence of at least one alert sign, platelets count < 30 × 109/L, prothrombin time < 60%, AST and/or ALT > 10 N, and previous dengue infection. Severity was not influenced by the infecting dengue serotype nor by previous Zika infection.

Two models to predict dengue severity were built according to sex. Best models for females and males had respectively a median Area Under the Curve = 0.80 and 0.88, a sensitivity = 84.5 and 84.5%, a specificity = 78.6 and 95.5%, a positive predictive value = 63.3 and 92.9%, a negative predictive value = 92.8 and 91.3%. Models were secondarily validated on 130 patients hospitalized for dengue in 2018.

Conclusion

We built robust and efficient models to calculate a bedside score able to predict dengue severity in our setting. We propose the spreadsheet for dengue severity score calculations to health practitioners facing dengue outbreaks of enhanced severity in order to improve patients’ medical management and hospitalization flow.
Coding practice in national and regional kidney biopsy registries
Authors:   On behalf of the Kidney Biopsy Codes for Pathologists project (www.kibico.org), Dendooven Amélie , Peetermans Han , Helbert Mark , Nguyen Tri Q. , Marcussen Niels , Nagata Michio , Gesualdo Loreto , Perkowska-Ptasinska Agnieszka , Capusa Cristina , López-Gómez Juan M. , Geddes Colin , Abdul-Hamid Myrurgia A. , Segelmark Mårten , Yahya Rosnawati , Garau Mariela , Villanueva Russell , Dorman Anthony , Barbour Sean , Cornet Ronald , Hopfer Helmut , Amann Kerstin , Leh Sabine
Published: May 2021

Background

Kidney biopsy registries all over the world benefit research, teaching and health policy. Comparison, aggregation and exchange of data is however greatly dependent on how registration and coding of kidney biopsy diagnoses are performed. This paper gives an overview over kidney biopsy registries, explores how these registries code kidney disease and identifies needs for improvement of coding practice.

Methods

A literature search was undertaken to identify biopsy registries for medical kidney diseases. These data were supplemented with information from personal contacts and from registry websites. A questionnaire was sent to all identified registries, investigating age of registries, scope, method of coding, possible mapping to international terminologies as well as self-reported problems and suggestions for improvement.

Results

Sixteen regional or national kidney biopsy registries were identified, of which 11 were older than 10 years. Most registries were located either in Europe (10/16) or in Asia (4/16). Registries most often use a proprietary coding system (12/16). Only a few of these coding systems were mapped to SNOMED CT (1), older SNOMED versions (2) or ERA-EDTA PRD (3). Lack of maintenance and updates of the coding system was the most commonly reported problem.

Conclusions

There were large gaps in the global coverage of kidney biopsy registries. Limited use of international coding systems among existing registries hampers interoperability and exchange of data. The study underlines that the use of a common and uniform coding system is necessary to fully realize the potential of kidney biopsy registries.
A validation study of the kidney failure risk equation in advanced chronic kidney disease according to disease aetiology with evaluation of discrimination, calibration and clinical utility
Authors: Ali Ibrahim , Donne Rosemary L. , Kalra Philip A.
Published: May 2021

Background

The Kidney Failure Risk Equation (KFRE) predicts the 2- and 5-year risk of end-stage renal disease (ESRD) in patients with chronic kidney disease (CKD) stages 3a-5. Its predictive performance in advanced CKD and in specific disease aetiologies requires further exploration. This study validates the 4- and 8-variable KFREs in an advanced CKD population in the United Kingdom by evaluating discrimination, calibration and clinical utility.

Methods

Patients enrolled in the Salford Kidney Study who were referred to the Advanced Kidney Care Service (AKCS) clinic at Salford Royal NHS Foundation Trust between 2011 and 2018 were included. The 4- and 8-variable KFREs were calculated on the first AKCS visit and the observed events of ESRD (dialysis or pre-emptive transplantation) within 2- and 5-years were the primary outcome. The area under the receiver operator characteristic curve (AUC) and calibration plots were used to evaluate discrimination and calibration respectively in the whole cohort and in specific disease aetiologies: diabetic nephropathy, hypertensive nephropathy, glomerulonephritis, autosomal dominant polycystic kidney disease (ADPKD) and other diseases. Clinical utility was assessed with decision curve analyses, comparing the net benefit of using the KFREs against estimated glomerular filtration rate (eGFR) cut-offs of < 20 ml/min/1.73m2 and < 15 ml/min/1.73m2 to guide further treatment.

Results

A total of 743 patients comprised the 2-year analysis and 613 patients were in the 5-year analysis. Discrimination was good in the whole cohort: the 4-variable KFRE had an AUC of 0.796 (95% confidence interval [CI] 0.762–0.831) for predicting ESRD at 2-years and 0.773 (95% CI 0.736–0.810) at 5-years, and there was good-to-excellent discrimination across disease aetiologies. Calibration plots revealed underestimation of risk at 2-years and overestimation of risk at 5-years, especially in high-risk patients. There was, however, underestimation of risk in patients with ADPKD for all KFRE calculations. The predictive accuracy was similar between the 4- and 8-variable KFREs. Finally, compared to eGFR-based thresholds, the KFRE was the optimal tool to guide further care based on decision curve analyses.

Conclusions

The 4- and 8-variable KFREs demonstrate adequate discrimination and calibration for predicting ESRD in an advanced CKD population and, importantly, can provide better clinical utility than using an eGFR-based strategy to inform decision-making.
Efficacy and safety of rucaparib in previously treated, locally advanced or metastatic urothelial carcinoma from a phase 2, open-label trial (ATLAS)
Authors: Grivas P. , Loriot Y. , Morales-Barrera R. , Teo M. Y. , Zakharia Y. , Feyerabend S. , Vogelzang N. J. , Grande E. , Adra N. , Alva A. , Necchi A. , Rodriguez-Vida A. , Gupta S. , Josephs D. H. , Srinivas S. , Wride K. , Thomas D. , Simmons A. , Loehr A. , Dusek R. L. , Nepert D. , Chowdhury S.
Published: May 2021

Background

ATLAS evaluated the efficacy and safety of the PARP inhibitor rucaparib in patients with previously treated locally advanced/unresectable or metastatic urothelial carcinoma (UC).

Methods

Patients with UC were enrolled independent of tumor homologous recombination deficiency (HRD) status and received rucaparib 600 mg BID. The primary endpoint was investigator-assessed objective response rate (RECIST v1.1) in the intent-to-treat and HRD-positive (loss of genome-wide heterozygosity ≥10%) populations. Key secondary endpoints were progression-free survival (PFS) and safety. Disease control rate (DCR) was defined post-hoc as the proportion of patients with a confirmed complete or partial response (PR), or stable disease lasting ≥16 weeks.

Results

Of 97 enrolled patients, 20 (20.6%) were HRD-positive, 30 (30.9%) HRD-negative, and 47 (48.5%) HRD-indeterminate. Among 95 evaluable patients, there were no confirmed responses. However, reductions in the sum of target lesions were observed, including 6 (6.3%) patients with unconfirmed PR. DCR was 11.6%; median PFS was 1.8 months (95% CI, 1.6–1.9). No relationship was observed between HRD status and efficacy endpoints. Median treatment duration was 1.8 months (range, 0.1–10.1). Most frequent any-grade treatment-emergent adverse events were asthenia/fatigue (57.7%), nausea (42.3%), and anemia (36.1%). Of 64 patients with data from tumor tissue samples, 10 (15.6%) had a deleterious alteration in a DNA damage repair pathway gene, including four with a deleteriousBRCA1orBRCA2alteration.

Conclusions

Rucaparib did not show significant activity in unselected patients with advanced UC regardless of HRD status. The safety profile was consistent with that observed in patients with ovarian or prostate cancer.

Trial registration

This trial was registered inClinicalTrials.gov(NCT03397394). Date of registration: 12 January 2018. This trial was registered in EudraCT (2017–004166-10).
The association between triglyceride glucose index and depression: data from NHANES 2005–2018
Authors: Shi Yi-Yi , Zheng Rui , Cai Jie-Jie , Qian Song-Zan
Published: May 2021

Background

The association between triglyceride glucose (TyG) index and depression is unclear. We conducted this analysis to explore whether higher TyG index is associated with a higher odd of depression.

Methods

This was an observational study using data from the National Health and Nutrition Examination Survey (2005–2018), a cross-sectional and nationally representative database. Depression was assessed using the Patient Health Questionnaire-9 (PHQ-9). TyG index was calculated based on the equation as follows: ln [triglyceride (mg/dL) × fasting blood glucose (mg/dL)/2], and participants were divided into quartiles based on TyG index. Weighted multivariable logistic regression models were used to explore the relationship between the TyG index and depression.

Results

A total of 13,350 patients were included, involving 1001 (7.50%) individuals with depression. Higher TyG index is significantly associated with elevated depressive symptoms in U.S. adults. Multivariate-adjusted HRs for patients in the TyG index 4th quartile were higher for depression (OR = 1.46; 95% confidence interval (CI) 1.30, 1.64) compared with the 1st quartile of TyG index. Similar results were seen in men and women, across age groups, and baseline comorbidities.

Conclusion

In this large cross-sectional study, our result suggests that population with higher TyG index are significantly more likely to have depressive symptoms in U.S. adults.
Effects of wrist extension on median nerve and flexor tendon excursions in patients with carpal tunnel syndrome: a case control study
Authors: Liu Chien-Ting , Liu Dung-Huan , Chen Chii-Jen , Wang You-Wei , Wu Pao-Sheng , Horng Yi-Shiung
Published: May 2021

Background

Reduced gliding ability of the median nerve in the carpal tunnel has been observed in patients with carpal tunnel syndrome (CTS). The purpose of this study was to evaluate the gliding abilities of the median nerve and flexor tendon in patients with CTS and healthy participants in the neutral and 30° extended positions of the wrist and to compare the gliding between the finger flexion and extension phases.

Methods

Patients with CTS and healthy participants were consecutively recruited in a community hospital. All the subjects received the Boston CTS questionnaire, physical examinations, nerve conduction study (NCS), and ultrasonography of the upper extremities. Duplex Doppler ultrasonography was performed to evaluate the gliding abilities of the median nerve and flexor tendon when the subjects continuously moved their index finger in the neutral and 30° extension positions of the wrist.

Results

Forty-nine patients with CTS and 48 healthy volunteers were consecutively recruited. Significant differences in the Boston CTS questionnaire, physical examination and NCS results and the cross-sectional area of the median nerve were found between the patients and the healthy controls. The degree of median nerve gliding and the ratio of median nerve excursion to flexor tendon excursion in the CTS group were significantly lower than those in the healthy control group in both the neutral and 30° wrist extension positions. Significantly increased excursion of both the median nerve and flexor tendon from the neutral to the extended positions were found in the CTS group. The ratio of median nerve excursion to flexor tendon excursion was significantly higher in the finger flexion phase than in the extended phase in both groups, and this ratio had mild to moderate correlations with answers on the Boston CTS Questionnaire and with the NCS results.

Conclusions

Reduced excursion of the median nerve was found in the patients with CTS. The ratio of median nerve excursion to flexor tendon excursion was significantly lower in the patients with CTS than in the healthy volunteers. The median nerve excursion was increased while the wrist joint was extended to 30° in the patients with CTS. Wrist extension may be applied as part of the gliding exercise regimen for patients with CTS to improve median nerve mobilization.
Microsurgical training course for clinicians and scientists: a 10-year experience at the Münster University Hospital
Authors: Juratli Mazen A. , Becker Felix , Palmes Daniel , Stöppeler Sandra , Bahde Ralf , Kebschull Linus , Spiegel Hans-Ullrich , Hölzen Jens P.
Published: May 2021

Background

Microsurgical techniques are an important part of clinical and experimental research. Here we present our step-by-step microsurgery training course developed at the Münster University Hospital. The goal of this course was to create a short, modular curriculum with clearly described and easy to follow working steps in accordance with the Guidelines for Training in Surgical Research in Animals by the Academy of Surgical Research.

Methods

Over the course of 10 years, we conducted an annual 2.5 day (20 h) microsurgical training course with a total of 120 participants.

Results

Prior to the course, 90% of the participants reported to have never performed a microanastomosis before. During the 10 years a total of 84.2% of the participants performed microanastomoses without assistance, 15% required assistance and only 0.8% failed.

Conclusions

Our step-by-step microsurgery training course gives a brief overview of the didactic basics and the organization of a microsurgical training course and could serve as a guide for teaching microsurgical skills. During the 2.5-day curriculum, it was possible to teach, and for participants to subsequently perform a microsurgical anastomosis. The independent reproducibility of the learned material after the course is not yet known, therefore further investigations are necessary. With this step-by-step curriculum, we were able to conduct a successful training program, shown by the fact that each participant is able to perform microvascular anastomoses on a reproducible basis.
Honeybee communication during collective defence is shaped by predation
Authors: López-Incera Andrea , Nouvian Morgane , Ried Katja , Müller Thomas , Briegel Hans J.
Published: May 2021

Background

Social insect colonies routinely face large vertebrate predators, against which they need to mount a collective defence. To do so, honeybees use an alarm pheromone that recruits nearby bees into mass stinging of the perceived threat. This alarm pheromone is carried directly on the stinger; hence, its concentration builds up during the course of the attack. We investigate how bees react to different alarm pheromone concentrations and how this evolved response pattern leads to better coordination at the group level.

Results

We first present a dose-response curve to the alarm pheromone, obtained experimentally. This data reveals two phases in the bees’ response: initially, bees become more likely to sting as the alarm pheromone concentration increases, but aggressiveness drops back when very high concentrations are reached. Second, we apply Projective Simulation to model each bee as an artificial learning agent that relies on the pheromone concentration to decide whether to sting or not. Individuals are rewarded based on the collective performance, thus emulating natural selection in these complex societies. By also modelling predators in a detailed way, we are able to identify the main selection pressures that shaped the response pattern observed experimentally. In particular, the likelihood to sting in the absence of alarm pheromone (starting point of the dose-response curve) is inversely related to the rate of false alarms, such that bees in environments with low predator density are less likely to waste efforts responding to irrelevant stimuli. This is compensated for by a steep increase in aggressiveness when the alarm pheromone concentration starts rising. The later decay in aggressiveness may be explained as a curbing mechanism preventing worker loss.

Conclusions

Our work provides a detailed understanding of alarm pheromone responses in honeybees and sheds light on the selection pressures that brought them about. In addition, it establishes our approach as a powerful tool to explore how selection based on a collective outcome shapes individual responses, which remains a challenging issue in the field of evolutionary biology.
Prevalence, virulence factor and antimicrobial resistance analysis of Salmonella Enteritidis from poultry and egg samples in Iran
Authors: Bahramianfard Hassan , Derakhshandeh Abdollah , Naziri Zahra , Khaltabadi Farahani Reza
Published: May 2021

Background

Salmonella enterica serovar Enteritidis (S. Enteritidis) is one of the most common serovars, associated with human salmonellosis. The food-borne outbreak of this bacterium is mainly related to the consumption of contaminated poultry meat and poultry products, including eggs. Therefore, rapid and accurate detection, besides investigation of virulence characteristics and antimicrobial resistance profiles of S. Enteritidis in poultry and poultry egg samples is essential. A total of 3125 samples (2250 poultry and 875 poultry egg samples), sent to the administrative centers of veterinary microbiology laboratories in six provinces of Iran, were examined for Salmonella contamination, according to the ISO 6579 guideline. Next, duplex PCR was conducted on 250 presumptive Salmonella isolates to detect invA gene for identification of the genus Salmonella and sdf gene for identification of S. Enteritidis. Subsequently, the S. Enteritidis isolates were examined for detection of important virulence genes (pagC, cdtB, msgA, spaN, tolC, lpfC, and spvC) and determination of antibiotic resistance patterns against nalidixic acid, trimethoprim-sulfamethoxazole, cephalothin, ceftazidime, colistin sulfate, and kanamycin by the disk diffusion method.

Results

Overall, 8.7 and 2.3% of poultry samples and 6.3 and 1.3% of eggs were contaminated with Salmonella species and S. Enteritidis, respectively. The invA and msgA genes (100%) and cdtB gene (6.3%) had the highest and the lowest prevalence rates in S. Enteritidis isolates. The spvC gene, which is mainly located on the Salmonella virulence plasmid, was detected in 50.8% of S. Enteritidis isolates. The S. Enteritidis isolates showed the highest and the lowest resistance to nalidixic acid (87.3%) and ceftazidime (11.1%), respectively. Unfortunately, 27.0% of S. Enteritidis isolates were multidrug-resistant (MDR).

Conclusion

The rate of contamination with Salmonella in the poultry and egg samples, besides the presence of antimicrobial resistant and MDR Salmonella isolates harboring the virulence genes in these samples, could significantly affect food safety and subsequently, human health. Therefore, continuous monitoring of animal-source foods, enhancement of poultry farm control measures, and limiting the use of antibiotics for prophylactic purposes in food producing animals, are essential for reducing the zoonotic risk of this foodborne pathogen for consumers and also choosing effective antibiotics for the treatment of salmonellosis.
The benefits of an integrated social medical insurance for health services utilization in rural China: evidence from the China health and retirement longitudinal study
Authors: Fan Xiaojing , Su Min , Si Yafei , Zhao Yaxin , Zhou Zhongliang
Published: May 2021

Background

Improving health equity is a fundamental goal for establishing social health insurance. This article evaluated the benefits of the Integration of Social Medical Insurance (ISMI) policy for health services utilization in rural China.

Methods

Using the China Health and Retirement Longitudinal study (2011‒2018), we estimated the changes in rates and equity in health services utilization by a generalized linear mixed model, concentration curves, concentration indices, and a horizontal inequity index before and after the introduction of the ISMI policy.

Results

For the changes in rates, the generalized linear mixed model showed that the rate of inpatient health services utilization (IHSU) nearly doubled after the introduction of the ISMI policy (8.78 % vs. 16.58 %), while the rate of outpatient health services utilization (OHSU) decreased (20.25 % vs. 16.35 %) after the implementation of the policy. For the changes in inequity, the concentration index of OHSU decreased significantly from − 0.0636 (95 % CL: −0.0846, − 0.0430) before the policy to − 0.0457 (95 % CL: −0.0684, − 0.0229) after it. In addition, the horizontal inequity index decreased from − 0.0284 before the implementation of the policy to − 0.0171 after it, indicating that the inequity of OHSU was further reduced. The concentration index of IHSU increased significantly from − 0.0532 (95 % CL: −0.0868, − 0.0196) before the policy was implemented to − 0.1105 (95 % CL: −0.1333, − 0.0876) afterwards; the horizontal inequity index of IHSU increased from − 0.0066 before policy implementation to − 0.0595 afterwards, indicating that more low-income participants utilized inpatient services after the policy came into effect.

Conclusions

The ISMI policy had a positive effect on improving the rate of IHSU but not on the rate of OHSU. This is in line with this policy’s original intention of focusing on inpatient service rather than outpatients to achieve its principal goal of preventing catastrophic health expenditure. The ISMI policy had a positive effect on reducing the inequity in OHSU but a negative effect on the decrease in inequity in IHSU. Further research is needed to verify this change. This research on the effects of integration policy implementation may be useful to policy makers and has important policy implications for other developing countries facing similar challenges on the road to universal health coverage.
Validation and clinical evaluation of a Polish translation of the Wisconsin Upper Respiratory Symptom Survey for Kids (WURSS-K)
Authors: Ostrzyżek-Przeździecka Katarzyna , Panczyk Mariusz , Ratajczak Aleksandra , Bronikowski Michał , Feleszko Wojciech
Published: May 2021

Background

The Wisconsin Upper Respiratory Symptom Survey for Kids (WURSS-K) is a self-administered questionnaire developed to evaluate the severity of the common cold. It is a patient-oriented instrument that evaluates quality of life in an illness-specific manner to be used in children aged 10 years. The purpose of this study was to validate the Polish version of the Wisconsin Upper Respiratory Symptom Survey for Kids.

Methods

The validation process consisted of five stages: forward translation, backward translation, cognitive debriefing, a pilot study (Study A and Study B), and statistical analysis. The first study (Study A, n = 10, aged 5–13) was conducted in the Emergency Room and an Outpatient Clinic of the Pediatric University Hospital in Warsaw. The purpose of the study was to obtain data for testing the convergent validity of the questionnaire. The second study (Study B, n = 56), consisted of children aged four to six enrolled in three kindergartens in the Warsaw suburbs. The obtained data were subjected to detailed statistical analysis.

Results

The WURSS Kids Polish showed excellent reliability. The Cronbach’s alpha of the 13 items was 0.791 for the six symptom items and 0.854 for the seven functional items. The Jonckheere–Terpstra trend test was used to evaluate criterion validity. Compliance of the measurement performed independently by the examined person and the doctor on the first day was high (convergent validity). Each particular item was characterized by a different sensitivity to clinical change. The Guyatt’s Responsiveness index ranged from 0.083 to 0.464.

Conclusion

The internal consistency of the measurements and cross-cultural adaptation of the Polish version of WURSS Kids was satisfactory. The WURSS Kids Polish is a reliable, valid, and responsive disease-specific questionnaire for assessing symptoms and QOL in Polish patients in the pediatric population with the common cold. It may be used both in clinical practice and for research among Polish children with URTI.
Pan-cancer characterization of metabolism-related biomarkers identifies potential therapeutic targets
Authors: Bi Guoshu , Bian Yunyi , Liang Jiaqi , Yin Jiacheng , Li Runmei , Zhao Mengnan , Huang Yiwei , Lu Tao , Zhan Cheng , Fan Hong , Wang Qun
Published: May 2021

Background

Generally, cancer cells undergo metabolic reprogramming to adapt to energetic and biosynthetic requirements that support their uncontrolled proliferation. However, the mutual relationship between two critical metabolic pathways, glycolysis and oxidative phosphorylation (OXPHOS), remains poorly defined.

Methods

We developed a “double-score” system to quantify glycolysis and OXPHOS in 9668 patients across 33 tumor types from The Cancer Genome Atlas and classified them into four metabolic subtypes. Multi-omics bioinformatical analyses was conducted to detect metabolism-related molecular features.

Results

Compared with patients with low glycolysis and high OXPHOS (LGHO), those with high glycolysis and low OXPHOS (HGLO) were consistently associated with worse prognosis. We identified common dysregulated molecular features between different metabolic subgroups across multiple cancers, including gene, miRNA, transcription factor, methylation, and somatic alteration, as well as investigated their mutual interfering relationships.

Conclusion

Overall, this work provides a comprehensive atlas of metabolic heterogeneity on a pan-cancer scale and identified several potential drivers of metabolic rewiring, suggesting corresponding prognostic and therapeutic utility.
Towards a Verification-Driven Iterative Development of Software for Safety-Critical Cyber-Physical Systems
Authors: Sirjani Marjan , Provenzano Luciana , Asadollah Sara Abbaspour , Moghadam Mahshid Helali , Saadatmand Mehrdad
Published: May 2021

Software systems are complicated, and the scientific and engineering methodologies for software development are relatively young. Cyber-physical systems are now in every corner of our lives, and we need robust methods for handling the ever-increasing complexity of their software systems. Model-Driven Development is a promising approach to tackle the complexity of systems through the concept of abstraction, enabling analysis at earlier phases of development. In this paper, we propose a model-driven approach with a focus on guaranteeing safety using formal verification. Cyber-physical systems are distributed, concurrent, asynchronous and event-based reactive systems with timing constraints. The actor-based textual modeling language, Rebeca, with model checking support is used for formal verification. Starting from structured requirements and system architecture design the behavioral models, including Rebeca models, are built. Properties of interest are also derived from the structured requirements, and then model checking is used to formally verify the properties. This process can be performed in iterations until satisfaction of desired properties are ensured, and possible ambiguities and inconsistencies in requirements are resolved. The formally verified models can then be used to develop the executable code. The Rebeca models include the details of the signals and messages that are passed at the network level including the timing, and this facilitates the generation of executable code. The natural mappings among the models for requirements, the formal models, and the executable code improve the effectiveness and efficiency of the approach.
Predict Alzheimer’s disease using hippocampus MRI data: a lightweight 3D deep convolutional network model with visual and global shape representations
Authors: Katabathula Sreevani , Wang Qinyong , Xu Rong
Published: May 2021

Background

Alzheimer’s disease (AD) is a progressive and irreversible brain disorder. Hippocampus is one of the involved regions and its atrophy is a widely used biomarker for AD diagnosis. We have recently developed DenseCNN, a lightweight 3D deep convolutional network model, for AD classification based on hippocampus magnetic resonance imaging (MRI) segments. In addition to the visual features of the hippocampus segments, the global shape representations of the hippocampus are also important for AD diagnosis. In this study, we propose DenseCNN2, a deep convolutional network model for AD classification by incorporating global shape representations along with hippocampus segmentations.

Methods

The data was obtained from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) and was T1-weighted structural MRI from initial screening or baseline, including ADNI 1,2/GO and 3. DenseCNN2 was trained and evaluated with 326 AD subjects and 607 CN hippocampus MRI using 5-fold cross-validation strategy. DenseCNN2 was compared with other state-of-the-art machine learning approaches for the task of AD classification.

Results

We showed that DenseCNN2 with combined visual and global shape features performed better than deep learning models with visual or global shape features alone. DenseCNN2 achieved an average accuracy of 0.925, sensitivity of 0.882, specificity of 0.949, and area under curve (AUC) of 0.978, which are better than or comparable to the state-of-the-art methods in AD classification. Data visualization analysis through 2D embedding of UMAP confirmed that global shape features improved class discrimination between AD and normal.

Conclusion

DenseCNN2, a lightweight 3D deep convolutional network model based on combined hippocampus segmentations and global shape features, achieved high performance and has potential as an efficient diagnostic tool for AD classification.
InChI version 1.06: now more than 99.99% reliable
Authors: Goodman Jonathan M. , Pletnev Igor , Thiessen Paul , Bolton Evan , Heller Stephen R.
Published: May 2021

The software for the IUPAC Chemical Identifier, InChI, is extraordinarily reliable. It has been tested on large databases around the world, and has proved itself to be an essential tool in the handling and integration of large chemical databases. InChI version 1.05 was released in January 2017 and version 1.06 in December 2020. In this paper, we report on the current state of the InChI Software, the details of the improvements in the v1.06 release, and the results of a test of the InChI run on PubChem, a database of more than a hundred million molecules. The upgrade introduces significant new features, including support for pseudo-element atoms and an improved description of polymers. We expect that few, if any, applications using the standard InChI will need to change as a result of the changes in version 1.06. Numerical instability was discovered for 0.002% of this database, and a small number of other molecules were discovered for which the algorithm did not run smoothly. On the basis of PubChem data, we can demonstrate that InChI version 1.05 was 99.996% accurate, and InChI version 1.06 represents a step closer to perfection. Finally, we look forward to future releases and extensions for the InChI Chemical identifier.
Global characteristics of the westward-propagating quasi-16-day wave with zonal wavenumber 1 and the connection with the 2012/2013 SSW revealed by ERA-Interim
Authors: Li Wei , Huang Chunming , Zhang Shaodong
Published: May 2021

We analyze global characteristics of the westward-propagating quasi-16-day wave (Q16DW) with zonal wavenumber 1 (W1) in the troposphere and stratosphere using zonal wind, meridional wind, vertical velocity, temperature, geopotential, and potential vorticity data from the European Centre for Medium-Range Weather Forecasts (ECMWF) Interim Re-Analysis during one year from December 2012 to November 2013. The amplitudes of the W1 Q16DW are larger in the stratosphere than in the troposphere, and remarkable amplitudes are found at middle and high latitudes in the Northern Hemisphere (NH). More detailed analyses on the temporal variation in the W1 Q16DW show that this wave is significantly enhanced during the 2012/2013 Stratospheric Sudden Warming (SSW) event, and the strong wave most likely provides additional forcing on the splitting of the displaced polar vortex. Analysis of the Eliassen–Palm flux (EP flux) and its divergence of the interaction between W1 Q16DW and quasi-stationary planetary waves with wavenumber 1 during the 2012/2013 SSW event reveals that it causes an upward heat flux and exerts a westward acceleration on the background winds, indicating that this interaction plays an important role in the eastward stratospheric jet reversal. Moreover, the wave is amplified in the occurrence region of barotropic and/or baroclinic instability, suggesting a local source of the growing W1 Q16DW during this SSW event.
A new geographic record of the endangered Telmatobufo venustus (Amphibia: Calyptocephalellidae) in the Biobío Region, Chile
Authors: Díaz-Páez Helen , Alveal Nicza
Published: May 2021

We document the record of Telmatobufo venustus Phillipi (1899) in the Altos de Malalcura sector, Antuco, Biobío Region, Chile. This is the first record metamorphic Gosner 45 stage and the fourth georeferenced record of the species throughout its distribution. The data extend the presence of the species in the Biobío Region by more than 100 km, extending T. venustus to north of the Ralco Reserve. The presence of three metamorphic stage individuals and their coexistence with Alsodes sp. in this locality stands out.
N,N-Dimethylglycine in patients with progressive multiple sclerosis: result of a pilot double-blind, placebo, controlled randomized clinical trial
Authors: Wolfsegger Thomas , Böck Klaus , Schimetta Wolfgang , von Oertzen Tim J. , Assar Hamid
Published: May 2021

Oral administration of N,N-Dimethylglycine (DMG), a tertiary amino acid, presumably enhances oxygen utilization by tissue and complex with free radicals. Beneficial effects are improved endurance performance and reduction fatigue in humans and animals. This pilot study reports the results over a one-year double-blind, placebo-controlled trial of DMG in 30 randomized patients with progressive multiple sclerosis. No treatment effects were found between the placebo group and the DMG group for disability, fatigue, cognitive, or gait parameters.