Potential Antibacterial Activity of Yemeni Sidr Honey Against Pseudomonas aeruginosa and Streptococcus pyogenes
Authors: Al-kafaween Mohammad A. , Hilmi Abu Bakar Mohd , Al-Jamal Hamid A. Nagi , Al-Groom Rania M. , Elsahoryi Nour A. , Al-Sayyed Hiba 
Published: March 2021

Background:

Sidr honey has been reported to exhibit antimicrobial activity against numerous
pathogenic bacteria making this honey a promising functional food for the treatment of
wounds or stomach ulcers.

Objective:

The purpose of this study was to investigate the effect of Sidr honey against P. aeruginosa
and S. pyogenes.

Methods:

Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC)
for Sidr honey were determined by the broth dilution method. The growth curve of both bacteria
with MIC, half-MIC, and quarter-MIC was monitored by optical density (at OD570). The timekill
curve was used to determine the bacteriostatic and bactericidal activity of Sidr honey on both
bacteria by plotting colony-forming unit (CFUs) versus time. The effect of Sidr honey on the ultrastructure
of the P. aeruginosa and S. pyogenes was investigated using scanning electron microscopy
(SEM). The effect of Sidr honey on the expression of virulence genes in both bacteria was determined
using quantitative reverse transcription-polymerase chain reaction (RT-qPCR).

Results:

The results showed that Sidr honey possessed the lowest MIC value against P. aeruginosa
and S. pyogenes with 12.5% (w/v) and 20% (w/v), respectively. In addition, the MBC value for Sidr
honey was found to be 20% (w/v) and 25% (w/v) against P. aeruginosa and S. pyogenes respectively.
Growth curves conducted with MIC Sidr honey resulted in no growth of P. aeruginosa and
S. pyogenes. Growth curves with half-MIC Sidr honey resulted in a reduced growth rate and reduction
in overall cell number in both bacteria over a period of 24 h, compared with cells grown without
honey. In the time-kill curve, treatment of P. aeruginosa and S. pyogenes with Sidr honey for 8
hours resulted in decreases of 4-log reduction (P<0.05) in total viable counts (TVCs). SEM analysis
revealed marked changes in the bacterial cell morphology for both bacteria following treatment
with Sidr honey. These changes included the appearance of irregular shapes, incomplete cell division,
and swelling cells. The RT-qPCR results showed that the expression of algD, oprF, fleN,
fleQ, fleR, fliA, and fliC in P. aeruginosa decreased 0.43-fold, 0.38-fold, 0.41-fold, 0.51-fold,
0.40-fold, 0.61-fold, and 0.39-fold respectively after exposure to Sidr honey. Meanwhile, the expression
of sof, sfbl, and scpA in S. pyogenes decreased 0.18-fold, 0.21-fold, and 0.28-fold respectively,
after treated with Sidr honey.

Conclusion:

Using varying methods to evaluate the planktonic integrity, this study demonstrated
that Sidr honey has antibacterial activity against both bacteria and has potential as a therapeutic
agent for microbial infection, particularly against these two organisms. To our knowledge, this
study is the first to indicate that Sidr honey is effective at inducing cell lysis and identify target
genes at the genetic level that might be involved in this process.