bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Validation of a Stable Isotope Dilution Headspace–SPME–GC/MS Method for the Determination of Vanillin in Fragrant Vegetable Oils
Authors: Mei Fangyi , Wang Hongling , Zhang Yuquan , Zhang Mei , Zhou Shuai , Shi Haiming , Jiang Yuanrong
Published: October 2023

It has been reported that vanillin has been intentionally added to enhance the taste and flavor of low-quality vegetable oils. Therefore, it is crucial to investigate the accurate concentrations of vanillin in three types of fragrant vegetable oils commonly consumed in China. In this study, a method has been developed for the quantification of vanillin in commercial fragrant vegetable oils using the stable isotope dilution assay (SIDA) and headspace–solid-phase microextraction (HS–SPME) coupled with gas chromatography/mass spectrometry (GC/MS). The limit of detection (LOD) and limit of quantification (LOQ) of the analyte were determined to be 20 µg kg−1 and 50 µg kg−1, respectively. The validation study demonstrated that the recoveries ranged from 89% to 101%, with intra-day and inter-day precision being less than 7.46%. A survey of 80 commercially available fragrant vegetable oils was performed using the present method. Vanillin was found to be widely present in fragrant vegetable oils, with sesame oils showing the highest average content (842.6 µg kg−1), followed by rapeseed oils (262.1 µg kg−1) and peanut oils (115.0 µg kg−1). The results indicate that the proposed method is a simple, accurate, and eco-friendly approach for determining the presences of vanillin in fragrant vegetable oils.
Anti-Inflammatory Activity of 1,6,7-Trihydroxy-2-(1,1-dimethyl-2-propenyl)-3-methoxyxanthone Isolated from Cudrania tricuspidata via NF-κB, MAPK, and HO-1 Signaling Pathways in Lipopolysaccharide-Stimulated RAW 264.7 and BV2 Cells
Authors: Ko Wonmin , Baek Jong-Suep , Liu Zhiming , Dong Linsha , Kim Nayeon , Lee Hwan , Yoon Chi-Su , Kim Na Young , Kim Sam Cheol , Lee Dong-Sung
Published: October 2023

Neuroinflammation activated by microglia affects inflammatory pain development. This study aimed to explore the anti-inflammatory properties and mechanisms of 1,6,7-trihydroxy-2-(1,1-dimethyl-2-propenyl)-3-methoxyxanthone (THMX) from Cudrania tricuspidata in microglia activation-mediated inflammatory pain. In RAW 264.7 and BV2 cells, THMX has been shown to reduce lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory mediators and cytokines, including nitric oxide (NO), prostaglandin (PG) E2, interleukin (IL)-6, and tumor necrosis factor alpha (TNF-α). THMX also decreased LPS-induced phosphorylation of mitogen-activated protein kinase (MAPK) and the activation of p65 nuclear factor kappa B (NF-κB). Interestingly, THMX also activated heme oxygenase (HO)-1 expression. These findings suggest that THMX is a promising biologically active compound against inflammation through preventing MAPKs and NF-ĸB and activating HO-1 signaling pathways.
A New Stereoselective Approach to the Substitution of Allyl Hydroxy Group in para-Mentha-1,2-diol in the Search for New Antiparkinsonian Agents
Authors: Podturkina Alexandra V. , Ardashov Oleg V. , Volcho Konstantin P. , Salakhutdinov Nariman F.
Published: October 2023

Two approaches to the synthesis of para-menthene epoxide ((1S,5S,6R)-4) are developed. The first approach includes a reaction between chlorohydrin 7 and NaH in THF. The second involves the formation of epoxide in the reaction of corresponding diacetate 6 with sodium tert-butoxide. One possible mechanism of this reaction is proposed to explain unexpected outcomes in the regio- and stereospecificity of epoxide (1S,5S,6R)-4 formation. The epoxide ring in (1S,5S,6R)-4 is then opened by various S- and O-nucleophiles. This series of reactions allows for the stereoselective synthesis of diverse derivatives of the monoterpenoid Prottremine 1, a compound known for its antiparkinsonian activity, including promising antiparkinsonian properties.
A Quick Method for Predicting Reflectance Spectra of Nanophotonic Devices via Artificial Neural Network
Authors: Wang Rui , Zhang Baicheng , Wang Guan , Gao Yachen
Published: October 2023

Nanophotonics use the interaction between light and subwavelength structures to design nanophotonic devices and to show unique optical, electromagnetic, and acoustic properties that natural materials do not have. However, this usually requires considerable expertise and a lot of time-consuming electromagnetic simulations. With the continuous development of artificial intelligence, people are turning to deep learning for designing nanophotonic devices. Deep learning models can continuously fit the correlation function between the input parameters and output, using models with weights and biases that can obtain results in milliseconds to seconds. In this paper, we use finite-difference time-domain for simulations, and we obtain the reflectance spectra from 2430 different structures. Based on these reflectance spectra data, we use neural networks for training, which can quickly predict unseen structural reflectance spectra. The effectiveness of this method is verified by comparing the predicted results to the simulation results. Almost all results maintain the main trend, the MSE of 94% predictions are below 10−3, all are below 10−2, and the MAE of 97% predictions are below 2 × 10−2. This approach can speed up device design and optimization, and provides reference for scientific researchers.
Dual-Band and Multi-State Polarization Conversion Using aTerahertz Symmetry-Breaking Metadevice
Authors: Deng Yuwang , Zhou Qingli , Zhang Xuteng , Zhang Pujing , Liang Wanlin , Ning Tingyin , Shi Yulei , Zhang Cunlin
Published: October 2023

We numerically and experimentally demonstrate a terahertz metadevice consisting of split-ring resonators (SRRs) present within square metallic rings. This device can function as a dual-band polarization converter by breaking the symmetry of SRRs. Under x-polarized incidence, the metastructure is able to convert linearly polarized (LP) light into a left-hand circular-polarized (LCP) wave. Intriguingly, under y-polarized incidence, frequency-dependent conversion from LP to LCP and right-hand circular-polarized (RCP) states can be achieved at different frequencies. Furthermore, reconfigurable LCP-to-LP and RCP-to-LP switching can be simulated by integrating the device with patterned graphene and changing its Fermi energy. This dual-band and multi-state polarization control provides an alternative solution to developing compact and multifunctional components in the terahertz regime.
Metal Oxide Nanostructures Enhanced Microfluidic Platform for Efficient and Sensitive Immunofluorescence Detection of Dengue Virus
Authors: Pormrungruang Pareesa , Phanthanawiboon Supranee , Jessadaluk Sukittaya , Larpthavee Preeda , Thaosing Jiraphon , Rangkasikorn Adirek , Kayunkid Navaphun , Waiwijit Uraiwan , Horprathum Mati , Klamchuen Annop , Pruksamas Tanapan , Puttikhunt Chunya , Yasui Takao , Djamal Mitra , Rahong Sakon , Nukeaw Jiti
Published: October 2023

Rapid and sensitive detection of Dengue virus remains a critical challenge in global public health. This study presents the development and evaluation of a Zinc Oxide nanorod (ZnO NR)-surface-integrated microfluidic platform for the early detection of Dengue virus. Utilizing a seed-assisted hydrothermal synthesis method, high-purity ZnO NRs were synthesized, characterized by their hexagonal wurtzite structure and a high surface-to-volume ratio, offering abundant binding sites for bioconjugation. Further, a comparative analysis demonstrated that the ZnO NR substrate outperformed traditional bare glass substrates in functionalization efficiency with 4G2 monoclonal antibody (mAb). Subsequent optimization of the functionalization process identified 4% (3-Glycidyloxypropyl)trimethoxysilane (GPTMS) as the most effective surface modifier. The integration of this substrate within a herringbone-structured microfluidic platform resulted in a robust device for immunofluorescence detection of DENV-3. The limit of detection (LOD) for DENV-3 was observed to be as low as 3.1 × 10−4 ng/mL, highlighting the remarkable sensitivity of the ZnO NR-integrated microfluidic device. This study emphasizes the potential of ZnO NRs and the developed microfluidic platform for the early detection of DENV-3, with possible expansion to other biological targets, hence paving the way for enhanced public health responses and improved disease management strategies.
Association of Maternal Diet with Infant Birthweight in Women with Gestational Diabetes Mellitus
Authors: Apostolopoulou Aikaterini , Tranidou Antigoni , Chroni Violeta , Tsakiridis Ioannis , Magriplis Emmanuella , Dagklis Themistoklis , Chourdakis Michail
Published: October 2023

Objective: This study aimed to explore the potential impact of pre-pregnancy and early pregnancy maternal nutrition on the incidence of small-for-gestational-age neonates (SGA) in women with gestational diabetes mellitus (GDM). Methods: A prospective cohort study was conducted between 2020 and 2022 at the 3rd Department of Obstetrics and Gynaecology (School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece). Pregnant women from routine care were surveyed about their dietary habits during two distinct periods: six months prior to pregnancy (period A) and from the onset of pregnancy until the oral glucose tolerance test at 24–28 gestational weeks (period B). The intake of various micronutrients and macronutrients was quantified from the questionnaire responses. Logistic regression models, adjusted for potential confounders including age, pre-pregnancy body mass index (BMI), smoking status, physical activity and parity, were used to evaluate the association between nutrient intake and small-for-gestational-age neonate incidence. Results: In total, 850 women were screened and of these, 90 (11%) were diagnosed with gestational diabetes mellitus and were included in the study. There were significant associations between the intake of specific nutrients and the occurrence of small-for-gestational-age neonates; higher fat intake compared to non-small for gestationa age during period B (aOR: 1.1, p = 0.005) was associated with an increased risk for small-for-gestational-age neonates, while lower intake of carbohydrates (g) (aOR: 0.95, p = 0.005), fiber intake (aOR: 0.79, p = 0.045), magnesium (aOR: 0.96, p = 0.019), and copper (aOR:0.01, p = 0.018) intake during period B were significantly associated with a decreased risk for small-for-gestational-age neonates. Conclusions: The findings of this study highlight the potential role of maternal nutrition in modulating the risk of small for gestational age neonatesamong women with gestational diabetes mellitus. The results advocate for further research on the assessment and modification of both pre-pregnancy and early pregnancy nutrition for women, especially those at higher risk of gestational diabetes mellitus, to reduce the risk of gestational diabetes mellitus.
“Marketing through Claims”: A Cross-Sectional Analysis of Child-Targeted Food Packaging Claims within the Spanish Market
Authors: Arraztio-Cordoba Alazne , Montero-Simo Maria Jose , Araque-Padilla Rafael A.
Published: October 2023

Food advertising, especially on packaging, impacts children’s choices. Food companies make different claims on packaging as a marketing techniques to make their products more appealing, enhancing their perceived healthiness, even in unhealthy products. Although the use of some claims is regulated, there are legal loopholes that could confuse young consumers and that concern global authorities. To shed light on the matter, it is necessary to have a comprehensive understanding of the performance of all types of claims made by food companies in food products targeting children. We examined 458 products from Spanish markets in two periods through a cross-sectional content analysis. Our findings reveal that existing policies are working due to the decrease in nutrition claims, but there is a growth in soft claims that are unregulated and potentially confusing and attractive to children. Considering that most of the products analyzed are unhealthy, this emphasizes the importance of implementing stricter regulations to create a healthier and more reliable food environment for children.
Effect of Lactobacillus kefiri, in Conjunction with PENS T6 and a Hypocaloric Diet, on Weight Loss, Hypertension and Laboratory Glycemic and Lipid Profile
Authors: Ruiz-Tovar Jaime , Llavero Carolina , Fernandez-Contreras Maria-Encarnacion
Published: October 2023

The pathogenesis of obesity has been linked to alterations in gut microorganisms. The aim of this study was to investigate the effect of Lactobacillus kefiri, together with PENS T6 and a hypocaloric diet, on weight loss, hypertension and laboratory glycemic and lipid profile. A prospective non-randomized study was conducted involving adult patients with a body mass index (BMI) > 30 kg/m2. Patients were divided into two groups: those undergoing PENS-T6 and hypocaloric diet (PENS-Diet Group) and those undergoing the same PENS-T6 scheme and hypocaloric diet, but additionally receiving probiotics including Lactobacillus kefiri (PENS-Diet + L. kefiri Group). Weight loss was assessed at the end of the treatment, and analytical glycemic and lipid profile, and microbiological analysis of feces were performed before and after treatment. The addition of Lactobacillus kefiri to PENS T6 and a low-calorie diet, increases weight loss and further improves the glycemic and lipid profile. L. kefiri also causes a further improvement in obesity-associated dysbiosis, mainly by increasing the muconutritive (Akkermansia muciniphila) and regulatory (Bifidobacterium spp.) microbiome, and the Phylum Bacteroidetes (Prevotella spp.) and decreasing the Firmicutes/Bacteroidetes ratio.
Eight Weeks of Bifidobacterium lactis BL-99 Supplementation Improves Lipid Metabolism and Sports Performance through Short-Chain Fatty Acids in Cross-Country Skiers: A Preliminary Study
Authors: Li Tieying , Rui Zihan , Mao Letian , Chang Yashan , Shao Jing , Chen Yue , Han Qi , Sui Xuemei , An Nan , Li Haoqiu , Feng Haotian , Jiang Tao , Wang Qirong
Published: October 2023

(1) Background: Probiotics in the form of nutritional supplements are safe and potentially useful for strategic application among endurance athletes. Bifidobacterium animalis lactis BL-99 (BL-99) was isolated from the intestines of healthy Chinese infants. We combined plasma-targeted metabolomics and fecal metagenomics to explore the effect of 8 weeks of BL-99 supplementation on cross-country skiers’ metabolism and sports performance. (2) Methods: Sixteen national top-level male cross-country skiers were recruited and randomly divided into a placebo group (C) and a BL-99 group (E). The participants took the supplements four times/day (with each of three meals and at 21:00) consistently for 8 weeks. The experiment was conducted in a single-blind randomized fashion. The subject’s dietary intake and total daily energy consumption were recorded. Blood and stool samples were collected before and after the 8-week intervention, and body composition, muscle strength, blood biochemical parameters, plasma-targeted metabolomic data, and fecal metagenomic data were then analyzed. (3) Results: The following changes occurred after 8 weeks of BL-99 supplementation: (a) There was no significant difference in the average total daily energy consumption and body composition between the C and E groups. (b) The VO2max and 60°/s and 180°/s knee joint extensor strength significantly increased in both the C and E groups. By the eighth week, the VO2max and 60 s knee-joint extensor strength were significantly higher in the E group than in the C group. (c) The triglyceride levels significantly decreased in both the C and E groups. In addition, the LDL-C levels significantly decreased in the E group. (d) The abundance of Bifidobacterium animalis increased two-fold in the C group and forty-fold in the E group. (e) Plasma-targeted metabolomic analysis showed that, after eight weeks of BL-99 supplementation, the increases in DHA, adrenic acid, linoleic acid, and acetic acid and decreases in glycocholic acid and glycodeoxycholic acid in the E group were significantly higher than those in the C group. (f) Spearman correlation analysis showed that there was a significant positive correlation between Bifidobacterium animalis’ abundance and SCFAs, PUFAs, and bile acids. (g) There was a significant correlation between the most significantly regulated metabolites and indicators related to sports performance and lipid metabolism. (4) Conclusions: Eight weeks of BL-99 supplementation combined with training may help to improve lipid metabolism and sports performance by increasing the abundance of Bifidobacterium, which can promote the generation of short-chain fatty acids and unsaturated fatty acids, and inhibit the synthesis of bile acids.
The Effect of Dietary Supplementation with Plant Sterols on Total and LDL-Cholesterol in Plasma Is Affected by Adherence to Mediterranean Diet: Insights from the DESCO Randomized Clinical Study
Authors: Cicero Arrigo F. G. , Fogacci Federica , Giovannini Marina , Rizzoli Elisabetta , Grandi Elisa , D’Addato Sergio , Borghi Claudio
Published: October 2023

Plant sterols are well-known natural lipid-lowering agents. The DESCO (Diet and plant sterols in the control of cholesterolemia) study was a single-center, randomized, double-blind, placebo-controlled, two-way crossover clinical trial designed to investigate the effect of a once-a-day ready-to-drink dietary supplement containing 2.5 g of phytosterols on the lipid profile, also in relation to the quality of the diet, in a cohort of 50 Italian individuals with polygenic hypercholesterolemia and low global cardiovascular risk. Eligible individuals were enrolled in a run-in period of 2 weeks. Then, participants who qualified for continuation in the study were randomly allocated (1:1) to a 3-week treatment with either phytosterols or placebo. After a 2-week washout period, enrolled individuals were crossed over to receive the alternative treatment. Dietary supplementation with phytosterols was associated with significant improvement in plasma levels of total cholesterol (TC; −11.8 ± 4.0 mg/dL, p = 0.016), low-density lipoprotein cholesterol (LDL-C; −7.8 ± 7.7 mg/dL, p = 0.021), and apolipoprotein B-100 (Apo B-100, −3.7 ± 4.1 mg/dL, p = 0.048) compared to baseline. The changes in TC and LDL-C were also significant compared to placebo, and greater adherence to the Mediterranean diet was significantly associated with greater reductions in LDL-C. Dietary supplementation with phytosterols was well tolerated and adherence to treatment was high. According to the findings of DESCO, the once-a-day ready-to-drink dietary supplement we tested is able to quickly and significantly decrease plasma levels of TC, LDL-C, and Apo B-100, with a greater effect in individuals more adhering to the Mediterranean dietary pattern.
Common Beans as a Source of Amino Acids and Cofactors for Collagen Biosynthesis
Authors: Añazco Carolina , Ojeda Paola G. , Guerrero-Wyss Marion
Published: October 2023

Common beans (Phaseolus vulgaris L.) are widely consumed in diets all over the world and have a significant impact on human health. Proteins, vitamins, minerals, phytochemicals, and other micro- and macronutrients are abundant in these legumes. On the other hand, collagens, the most important constituent of extracellular matrices, account for approximately 25–30 percent of the overall total protein composition within the human body. Hence, the presence of amino acids and other dietary components, including glycine, proline, and lysine, which are constituents of the primary structure of the protein, is required for collagen formation. In this particular context, protein quality is associated with the availability of macronutrients such as the essential amino acid lysine, which can be acquired from meals containing beans. Lysine plays a critical role in the process of post-translational modifications facilitated with enzymes lysyl hydroxylase and lysyl oxidase, which are directly involved in the synthesis and maturation of collagens. Furthermore, collagen biogenesis is influenced by the cellular redox state, which includes important minerals and bioactive chemicals such as iron, copper, and certain quinone cofactors. This study provides a novel perspective on the significant macro- and micronutrients present in Phaseolus vulgaris L., as well as explores the potential application of amino acids and cofactors derived from this legume in the production of collagens and bioavailability. The utilization of macro- and micronutrients obtained from Phaseolus vulgaris L. as a protein source, minerals, and natural bioactive compounds could optimize the capacity to promote the development and durability of collagen macromolecules within the human body.
Leucine Supplementation Exacerbates Morbidity in Male but Not Female Mice with Colorectal Cancer-Induced Cachexia
Authors: Schrems Eleanor R. , Haynie Wesley S. , Perry Richard A. , Morena Francielly , Cabrera Ana Regina , Rosa-Caldwell Megan E. , Greene Nicholas P. , Washington Tyrone A.
Published: October 2023

Cancer cachexia (CC) is a multifactorial wasting syndrome characterized by a significant loss in lean and/or fat mass and represents a leading cause of mortality in cancer patients. Nutraceutical treatments have been proposed as a potential treatment strategy to mitigate cachexia-induced muscle wasting. However, contradictory findings warrant further investigation. The purpose of this study was to determine the effects of leucine supplementation on skeletal muscle in male and female ApcMin/+ mice (APC). APC mice and their wild-type (WT) littermates were given normal drinking water or 1.5% leucine-supplemented water (n = 4–10/group/sex). We measured the gene expression of regulators of inflammation, protein balance, and myogenesis. Leucine treatment lowered survival rates, body mass, and muscle mass in males, while in females, it had no effect on body or muscle mass. Leucine treatment altered inflammatory gene expression by lowering Il1b 87% in the APC group and decreasing Tnfa 92% in both WT and APC males, while it had no effect in females (p < 0.05). Leucine had no effect on regulators of protein balance and myogenesis in either sex. We demonstrated that leucine exacerbates moribundity in males and is not sufficient for mitigating muscle or fat loss during CC in either sex in the ApcMin/+ mouse.
Operation of an Electrical-Only-Contact Photonic Integrated Chip for Quantum Random Number Generation Using Laser Gain-Switching
Authors: Ó Dúill Seán , Rodriguez Leidy , Alvarez-Outerelo David , Diaz-Otero Francisco , Sharma Ankit , Smyth Frank , Barry Liam
Published: October 2023

We present the results of the detected voltage distribution of a quantum random number generator (QRNG) based on a photonic integrated circuit comprising a semiconductor laser, delay interferometer and photodetector. We find that the integrated QRNG system behaves as expected for a QRNG from discrete gain-switched laser sources, especially exhibiting all of the peculiarities of the random voltage distribution and behaving as previously demonstrated for a discrete optical component setup. The biggest advantage of having all of the components integrated into a single chip is that only electrical connections are needed to operate the system, without the need for tricky and expensive optical alignment to external circuitry. We supply results showing that a random bit stream created from the random numbers passes the NIST statistical test suite tests, thus demonstrating the feasibility to generate random numbers via quantum means at gigabit/s rates from a single photonic integrated circuit. All of our results are backed by numerical simulations.
Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells
Authors: Ito Hiromu , Shoji Yoshimi , Ueno Megumi , Matsumoto Ken-ichiro , Nakanishi Ikuo
Published: October 2023

Radiotherapy, in which X-rays are commonly used, is one of the most effective procedures for treating cancer. However, some cancer cells become resistant to radiation therapy, leading to poor prognosis. Therefore, a new therapeutic method is required to prevent cancer cells from acquiring radiation resistance. Photodynamic therapy (PDT) is a cancer treatment that uses photosensitizers, such as porphyrin compounds, and low-powered laser irradiation. We previously reported that reactive oxygen species (ROS) derived from mitochondria induce the expression of a porphyrin transporter (HCP1) and that laser irradiation enhances the cytotoxic effect. In addition, X-ray irradiation induces the production of mitochondrial ROS. Therefore, radioresistant cancer cells established with continuous X-ray irradiation would also overexpress ROS, and photodynamic therapy could be an effective therapeutic method. In this study, we established radioresistant cancer cells and examined the therapeutic effects and mechanisms with photodynamic therapy. We confirmed that X-ray-resistant cells showed overgeneration of mitochondrial ROS and elevated expression of HCP1, which led to the active accumulation of porphyrin and an increase in cytotoxicity with laser irradiation. Thus, photodynamic therapy is a promising treatment for X-ray-resistant cancers.
Porphyrin as a Cryoprotectant for Graphene Oxide-Coated Gold Nanorods to Produce Conjugated Product with Improved Stability and Opto-Phototherapeutic Properties
Authors: Lebepe Thabang Calvin , Maluleke Rodney , Mgedle Nande , Oluwafemi Oluwatobi Samuel
Published: October 2023

Graphene oxide (GO) as a coating material for gold nanorods (AuNRs) has gained interest in reducing toxicity and improving the photothermal profiling of AuNRs. However, there is still a challenge regarding the storage of colloidal suspensions of GO-coated AuNRs (GO@AuNRs). Hence, the conjugation of GO@AuNRs to meso-tetra-(4-sulfonatophenyl)porphyrin (TPPS4), an anionic water-soluble porphyrin, has been reported to enhance their re-dispensability and improve their phototherapeutic properties. The AuNRs and GO were synthesised using seed-mediated and Hummers’ methods, respectively. The GO@AuNRs were conjugated to TPPS4 and characterised using ultraviolet–visible–near-infrared (UV-Vis-NIR) spectroscopy, zeta analyser, dynamic light scattering (DLS), photoluminescence spectroscopy (PL), x-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM) and Fourier-transform infrared spectroscopy (FTIR) before freeze-drying. The results showed that the AuNRs were sandwiched between GO and TPPS4. After freeze-drying, the freeze-dried conjugate was dispensed in deionised water without adding cryoprotectants and its properties were compared to those of the unfreeze-dried conjugate. The results showed that the freeze-dried conjugate contained similar optical properties to the unfreeze-dried conjugate. However, the bare GO@AuNRs showed a change in the optical properties after freeze-drying. These results revealed that porphyrin is an excellent additive to reduce the freeze-drying stress tolerance of GO@AuNRs. The freeze-dried conjugate also showed both singlet oxygen and photothermal properties of GO@AuNRs and porphyrin. These results indicated that the freeze-dried conjugate is a promising dual photodynamic and photothermal agent, and porphyrin can act as a cryoprotectant.
Selegiline Modulates Lipid Metabolism by Activating AMPK Pathways of Epididymal White Adipose Tissues in HFD-Fed Obese Mice
Authors: Joung Hye-Young , Oh Jung-Mi , Song Min-Suk , Kwon Young-Bae , Chun Sungkun
Published: October 2023

Obesity, as a major cause of many chronic diseases such as diabetes, cardiovascular disease, and cancer, is among the most serious health problems. Increased monoamine oxidase (MAO) activity has been observed in the adipose tissue of obese humans and animals. Although previous studies have already demonstrated the potential of MAO-B inhibitors as a treatment for this condition, the mechanism of their effect has been insufficiently elucidated. In this study, we investigated the anti-obesity effect of selegiline, a selective MAO-B inhibitor, using in vivo animal models. The effect was evaluated through an assessment of body energy homeostasis, glucose tolerance tests, and biochemical analysis. Pharmacological inhibition of MAO-B by selegiline was observed to reduce body weight and fat accumulation, and improved glucose metabolism without a corresponding change in food intake, in HFD-fed obese mice. We also observed that both the expression of adipogenenic markers, including C/EBPα and FABP4, and lipogenic markers such as pACC were significantly reduced in epididymal white adipose tissues (eWATs). Conversely, increased expression of lipolytic markers such as ATGL and pHSL and AMPK phosphorylation were noted. Treating obese mice with selegiline significantly increased expression levels of UCP1 and promoted eWAT browning, indicating increased energy expenditure. These results suggest that selegiline, by inhibiting MAO-B activity, is a potential anti-obesity treatment.
An Inverse Scaling Relationship between Stomatal Density and Mean Nearest Neighbor Distance: Evidence from a Photinia Hybrid and One of Its Parents
Authors: Sun Manli , Niinemets Ülo , Li Qiying , Jiao Yabing , Yao Weihao , Shi Peijian
Published: October 2023

Stomata are involved in transpiration and CO2 uptake by mediating gas exchange between internal plant tissues and the atmosphere. The capacity for gas exchange depends on stomatal density (SD), stomatal size, and pore dimensions. Most published work on stomatal quantification has assumed that stomatal distribution and stomatal density are spatially homogeneous across the leaf, but this assumption has been seldom tested. We selected 32 leaves from a Photinia hybrid, Photinia × fraseri ‘Red Robin’, and one of its parents, P. serratifolia. For each leaf, the leaf surface was divided into three or four equidistant layers along the apical–basal axis, and, in each layer, two positions, one closer to the midrib and the other closer to the leaf margin, were further selected. We calculated SD and mean nearest neighbor distance (MNND) for each lamina section and tested the scaling relationship between SD and MNND of the sampled stomatal centers using reduced major axis protocols. In addition, we calculated the stomatal aggregation index (SAI) for each lamina section to examine the spatial arrangement of stomata at the given size of field of view of 1.2 mm × 0.9 mm. We observed that SD decreased from the lamina apex towards the base for central lamina areas but varied little at leaf margins. An inverse scaling relationship between SD and MNND was observed for both species. This relationship could be used for SD estimation using the rapidly estimated trait, MNND. SAI did not vary significantly throughout leaf lamina, and the numerical values of SAI for all fields of view were greater than one, which indicates significant spatial repulsion between stomata. The study suggests that SD varies across leaf lamina to fine-tune plant water use and maximize carbon gain. However, spatial structures of stomata from different lamina sections exhibit similar patterns (i.e., spatial inhibition between stomata at small scales), probably due to hierarchical leaf vein patterns.
Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH
Authors: Qi Fei , Liang Shuang , Xing Piyi , Bao Yinguang , Wang Richard R.-C. , Li Xingfeng
Published: October 2023

The genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of Th. intermedium and its relative diploid species. St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum were used as probes in fluorescence in situ hybridization (FISH) to classify the chromosomes of Th. intermedium into three groups, expressed as JvsJvsJrJrStSt. A combined multiplex oligonucleotide probe, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten accessions of Th. intermedium. Variability among and within the studied accessions of intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that Jvs had largely been contributed from Da. breviaristatum, but not the present-day Da. villosum; IWG had only one J genome, Jr, which was related to either Th. elongatum or Th. bessarabicum; and St was contributed from the genus Pseudoroegneria by hybridization with Th. junceiforme or Th. sartorii.
Microanalysis of Active Nitrogen Oxides (RONS) Generation Characteristics during DC Negative Corona Discharge at a Needle-Plate Electrode
Authors: Shi Jinqiang , Jin Fubao , Ma Shangang , Liu Xinhe , Leng Xuejian , Chen Keyuan
Published: October 2023

The DC negative corona of needle-plate electrodes can generate atmospheric pressure low-temperature plasma active particles, which have important effects on biological mutagenesis. The DC negative corona discharge of an air needle-plate electrode with effective consideration of NOx particles was simulated and the Trichel pulse current was obtained, focusing on the development of particles and the distribution of active nitrogen oxides (RONS) at four moments in the pulse process. The simulation results indicate that the positive ions (N2+ and O2+) and negative ions (O− and O2−) were closely related to the current changes, and the negative ions (O− and O2−) presented a typical stratification phenomenon. RONS (H2O2, O3, and NO) were approximately uniformly distributed above the level of the plate electrode at the same instant, with H2O2 and O3 except for the area below the needle tip. They trended to a cumulative increase in concentration with time. This study provides a theoretical basis for corona discharge plasma seed treatment technology.
Chitosan Film Sensor for Ammonia Detection in Microdiffusion Analytical Devices
Authors: Tagliaro Irene , Musile Giacomo , Caricato Paolo , Dorizzi Romolo M. , Tagliaro Franco , Antonini Carlo
Published: October 2023

Chitosan films have attracted increased attention in the field of sensors because of chitosan’s unique chemico-physical properties, including high adsorption capacity, filmability and transparency. A chitosan film sensor was developed through the dispersion of an ammonia specific reagent (Nessler’s reagent) into a chitosan film matrix. The chitosan film sensor was characterized to assess the film’s properties by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). A gas diffusion device was prepared with the chitosan film sensor, enabling the collection and detection of ammonia vapor from biological samples. The chitosan film sensor color change was correlated with the ammonia concentration in samples of human serum and artificial urine. This method enabled facile ammonia detection and concentration measurement, making the sensor useful not only in clinical laboratories, but also for point-of-care devices and wherever there is limited access to modern laboratory facilities.
Strength and Surface Characteristics of 3D-Printed Resin Crowns for the Primary Molars
Authors: Park Soyoung , Cho Wontak , Lee Hyeonjong , Bae Jihyeon , Jeong Taesung , Huh Jungbo , Shin Jonghyun
Published: October 2023

Some resin polymers available for three-dimensional (3D) printing are slightly elastic, which may be advantageous when used for full crown coverage of the primary teeth. This study was performed to evaluate the mechanical properties of two types of 3D-printed resin crowns in terms of strength and surface characteristics. Polymer resins used for temporary crowns (TCs) and temporary flexible dentures (TFDs) were tested. Digitally designed crowns with different thicknesses (0.4 and 0.6 mm) were 3D-printed. Milled zirconia crowns were used as the control. The static and dynamic fracture loads of the crowns were measured. The crown surface was evaluated using scanning electron microscopy. The average strength did not differ between the types of crowns. The differences between the dynamic and static fracture loads were insignificant. In the TC group, thicker crowns showed lower strength both under static and dynamic loads. After thermomechanical loading, microcracks and dropouts of macrofillers were detected on the surface of all types of resin crowns. The deposition of abraded debris occurred more in the TFD group. The 3D-printed resin crowns were thought to endure biting forces in children. However, some limitations of the material itself should be improved for consideration as a new treatment option in pediatric dentistry.
Experimental Study on Enrichment of Indole in Wash Oil by a Combined Process of Extraction and Re-Extraction
Authors: Kim Su Jin
Published: October 2023

Wash oil, one of the by-products of coal tar distillation, contains useful nitrogen heterocyclic compounds (NHC) such as indole (IN), which is used as a raw material for perfume, essential amino acid, and pharmaceuticals. In this study, as a pre-treatment step for purification of IN, which is about 5.75% contained in wash oil, the enrichment of IN in wash oil was examined by combining formamide extraction (FE) and n-hexane re-extraction (HRE). The raw material for the re-extraction was a mixture of the preceding formamide extract phase acquired through batchwise 5-stage co-current extraction trial under fixed FE condition. As the 5-stage FE and HRE progressed, the IN yield based on IN in the wash oil was approximately 73.3%, and the IN concentration in the raffinate oil acquired from the re-extraction run was highly enriched to about 92.4%. In addition, the IN enrichment efficiency of the FE-HRE combined process (FHC) employed in this study was compared with methanol extraction (ME)-hexane re-extraction (HRE) combination (MHC).
Striving for a Complete Life: The Spiritual Essence of African–Americans’ Food Justice Activism
Authors: Johnson Lynn R.
Published: October 2023

This essay employs Dr Martin Luther King, Jr’s sermon, “The Three Dimensions of a Complete Life”, as an acute lens through which to assess and impart new meanings to African–American activists’ strivings to reach an ideal state of humanness and communal holism as they fulfilled their personal, political, and spiritual missions in the food realm during the 1960s Civil Rights era and the contemporary food justice movement. Narrative analyses of these Black activists’ personal testimonies convey that their discrete journeys to completeness—what Dr King called the ideal state of humanity in its fullness—were not only facilitated by a divine calling but were also conditioned by the enactment of their Christian faith, particularly in reconciling the affective tolls engendered by their participation in lunch-counter sit-ins and by their quests to help alleviate food insecurity among impoverish populations in the American South. Indeed, when these individuals consciously endeavored to master the three dimensions of a complete life—recognize their agency, honor the interconnectedness of humanity, and seek God’s guidance in doing both—were they able to embody their best selves and demand the realization of a truly democratic nation.
Experimental Investigation on Fragmentation Identification in Loose Slope Landslides by Infrared Emissivity Variability Features
Authors: Liu Xiangxin , Wu Lixin , Mao Wenfei , Sun Licheng
Published: October 2023

Infrared radiation (IR) features that are influenced by infrared emissivity ε and physical temperature Td have been successfully applied to the early-warning of landslides. Although the infrared emissivity of a rock is a key parameter to determine its thermal radiation properties, the effect of particle size on the infrared emissivity of rock fragments is unknown. So in this paper, granite, marble, and sandstone were used as examples to conduct infrared imaging experiments on rock fragments. Their equivalent emissivity was used to interpret the detected infrared emission, including that from indoor backgrounds. In addition, the characteristics of changes in equivalent emissivity were discussed with reference to changes in observation direction and zenith angle. Then, a computation model of equivalent emissivity based on multiple observation directions and zenith angles was built to reveal the change in equivalent emissivity with particle sizes. The result indicates that the indoor background radiation has a predominant direction just above the rock fragments. The maximum deviation of infrared brightness temperature (IBT) was 0.260 K, and the maximum deviation of equivalent emissivity among different observation directions and zenith angles was 0.0065. After eliminating the influence of directional and angle effects with the operation of normalization, the general law of equivalent emissivity for all rock fragments that change with particle size is consistent. The maximum equivalent emissivity occurs at particle size 5 mm in the condition of particle size larger than 1 mm, while the equivalent emissivity changes inversely with particle size in the condition of particle size smaller than 1 mm. Above all, this study contributes new cognitions to Remote Sensing Rock Mechanics, and provides valuable evidence for better thermal infrared remote sensing monitoring on loose slope landslides.
Comparison and Assessment of Different Land Cover Datasets on the Cropland in Northeast China
Authors: Cui Peipei , Chen Tan , Li Yingjie , Liu Kai , Zhang Dapeng , Song Chunqiao
Published: October 2023

The provision of precise and dependable information regarding the extent and distribution of cropland is imperative for the evaluation of food security, agricultural planning, and resource management. Cropland is an important component of land cover type and is offered in multiple existing global/regional land cover products. However, global-scale accuracy evaluation may not be representative of class-specific or local-area accuracy, such as in Northeast China, which is an important grain-producing region of China and has various types of cultivated land (e.g., wheat, rice) and diverse terrains. It poses a great challenge in generating precise cropland classification by automated mapping. Thus, it is indispensable to evaluate the accuracy and reliability of these various land cover datasets before using them. In this study, we collected thirteen sets of global or national-scale land cover datasets. Through the visual interpretation of high-resolution images, ground “truth” samples were collected to evaluate the data accuracy across Northeast China. The overall accuracy (OA) evaluation results in Phase-2020 show that CLCD has the highest value with 0.914, followed by GlobeLand30 (0.906), GLC_FCS30 (0.902), and Esri (0.896) for cropland classification in Northeast China. CGLS-LC100 has the lowest OA (0.710). For the commission and omission errors of six datasets in Phase-2020, CGLS-LC100 has an obvious overestimation (larger commission error), while the two national-scale datasets (CLCD and CLUDs) perform relatively better. In terms of spatial consistency, high spatial agreement among the nine Phase-2015 datasets or in the six Phase-2020 datasets could be discovered in traditional agricultural regions like the Sanjiang–Songnen–Liaohe Plain, and low agreement is found in the transition areas of mountains (hills) and plains with the mixed landscape of forest (grassland) and farmland. In the aspect of comparison pairwise data, CLCD is in good agreement with GLC_FCS30, GlobeLand30, and Esri, while CGLS-LC100 is in the poorest agreement with any other dataset. The comparison and evaluation results are expected to provide a reference on which aspects and to what extent these land cover products may be consistent and guide the cropland data product selection for Northeast China.
Rainfall-Induced Shallow Landslide Recognition and Transferability Using Object-Based Image Analysis in Brazil
Authors: Dias Helen Cristina , Hölbling Daniel , Grohmann Carlos Henrique
Published: October 2023

Landslides are among the most frequent hazards in Latin America and the world. In Brazil, they occur every year and cause economic and social loss. Landslide inventories are essential for assessing susceptibility, vulnerability, and risk. Over the decades, a variety of mapping approaches have been employed for the detection of landslides using Earth observation (EO) data. Object-based image analysis (OBIA) is a widely recognized method for mapping landslides and other morphological features. In Brazil, despite the high frequency of landslides, methods for inventory construction are poorly developed. The aim of this study is to semi-automatically recognize shallow landslides in Itaóca (Brazil) and evaluate the transferability of the approach within different areas in Brazil. RapidEye satellite images (5 m) and the derived normalized difference vegetation index (NDVI), as well as a digital elevation model (DEM) (12.5 m) and morphological data, were integrated into the classification. The results show that the method is suitable for the recognition of this type of hazard in Brazil. The overall accuracy was 89%. The main challenges were the identification of small landslides and the exact delineation of scars. The findings validate the applicability of the approach in Brazil, although additional adjustments to the primary rule set might lead to better results.
Forest Aboveground Biomass Estimation Using Machine Learning Ensembles: Active Learning Strategies for Model Transfer and Field Sampling Reduction
Authors: Amitrano Donato , Giacco Giovanni , Marrone Stefano , Pascarella Antonio Elia , Rigiroli Mattia , Sansone Carlo
Published: October 2023

Biomass is a crucial indicator of the carbon sequestration capacity of a vegetation ecosystem. Its dynamic is of interest because it impacts on the carbon cycle, which plays an important role in the global climate and its changes. This work presents a novel technique, able to transfer a calibrated regression model between different areas by exploiting an active learning methodology and using Shannon’s entropy as a discriminator for sample selection. Model calibration is performed based on a reference area for which an extended ground truth is available and implemented via regression bootstrap. Then, re-calibration samples for model transfer are selected through active learning, allowing for choosing a limited number of points to be investigated for training data collection. Different sampling strategies and regression techniques have been tested to demonstrate that a significant reduction in the number of calibration samples does not affect the estimation performance. The proposed workflow has been tested on a dataset concerning Finnish forests. Experimental results show that the joint exploitation of regression ensembles and active learning dramatically reduces the amount of field sampling, providing aboveground biomass estimates comparable to those obtained using literature techniques, which need extended training sets to build reliable predictions.