bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Vegetable Fields on the Spatial Distribution Patterns of Metal(loid)s in Soils Based on GIS and Moran’s I
Authors: Wang Qiang , Yang Shanlian , Zheng Menglei , Han Fengxiang , Ma Youhua
Published: October 2019

Metal(loid) pollution in vegetable field soils has become increasingly severe and affects the safety of vegetable crops. Research in China has mainly focused on greenhouse vegetables (GV), while open field vegetables (OV) and the spatial distribution patterns of metal(loid)s in the surrounding soils have rarely been assessed. In the present study, spatial analysis methods combining Geographic Information Systems (GIS) and Moran’s I were applied to analyze the effects of vegetable fields on metal(loid) accumulation in soils. Overall, vegetable fields affected the spatial distribution of metal(loid)s in soils. In long-term vegetable production, the use of large amounts of organic fertilizer led to the bioconcentration of cadmium (Cd) and mercury (Hg), and long-term fertilization resulted in a significant pH decrease and consequent transformation and migration of chromium (Cr), lead (Pb), and arsenic (As). Thus, OV fields with a long history of planting had lower average pH and Cd, and higher average As, Cr, Hg, and Pb than GV fields, reached 0.93%, 10.1%, 5.8%, 3.0%, 80.8%, and 0.43% respectively. Due to the migration and transformation of metal(loid)s in OV soils, these should be further investigated regarding their abilities to reduce the accumulation of metal(loid)s in soils and protect the quality of the cultivated land.
Status of Water Safety Plan Development and Implementation in Uganda
Authors: Kanyesigye Christopher , Marks Sara J. , Nakanjako Juliet , Kansiime Frank , Ferrero Giuliana
Published: October 2019

Uganda was among the first countries in Africa that pioneered Water Safety Plan (WSP) development and implementation, with the first WSP dating back to 2002. The objective of this study was to assess WSP status in Uganda, focusing on the experience of the National Water and Sewerage Corporation (NWSC), in order to understand the factors that influenced it and strategies for scaling-up. This study consisted of a review of documentation for 20 WSPs, 42 interviews, a focus group discussion and four field visits. Results show that the development of the 20 WSPs over the last 15 years was largely incomplete and diverse. Most of the WSPs focused on system assessment and improvement, but failed to include WSP monitoring, verification and management. The monitoring of control measures was implemented in nine of the 20 systems, while verification took place in the form of internal (5/20) and external (2/20) auditing. The main barriers identified to WSP implementation were inadequate training, team composition and deployment, mistaken perception and inability to evaluate WSP effectiveness. Conversely, the main enabling factors were management commitment, public health responsibility, good customer relations, financial availability and reliable laboratories. These findings suggest a need for more institutionalization of WSPs with improved coordination across stakeholder groups.
Six Collective Challenges for Sustainability of Almería Greenhouse Horticulture
Authors: Castro Antonio J. , López-Rodríguez María D. , Giagnocavo Cynthia , Gimenez Miguel , Céspedes Leticia , La Calle Abel , Gallardo Marisa , Pumares Pablo , Cabello Javier , Rodríguez Estefanía , Uclés David , Parra Salvador , Casas Jesús , Rodríguez Francisco , Fernandez-Prados Juan S. , Alba-Patiño Daniela , Expósito-Granados Mónica , Murillo-López Beatriz E. , Vasquez Lina M. , Valera Diego L.
Published: October 2019

Globally, current food consumption and trade are placing unprecedented demand on agricultural systems and increasing pressure on natural resources, requiring tradeoffs between food security and environmental impacts especially given the tension between market-driven agriculture and agro-ecological goals. In order to illustrate the wicked social, economic and environmental challenges and processes to find transformative solutions, we focus on the largest concentration of greenhouses in the world located in the semi-arid coastal plain of South-east Spain. Almería family farming, predominantly cooperative, greenhouse intensive production, commenced after the 1960s and has resulted in very significant social and economic benefits for the region, while also having important negative environmental and biodiversity impacts, as well as creating new social challenges. The system currently finds itself in a crisis of diminishing economic benefits and increasing environmental and social dilemmas. Here, we present the outcomes of multi-actor, transdisciplinary research to review and provide collective insights for solutions-oriented research on the sustainability of Almeria’s agricultural sector. The multi-actor, transdisciplinary process implemented collectively, and supported by scientific literature, identified six fundamental challenges to transitioning to an agricultural model that aims to ameliorate risks and avoid a systemic collapse, whilst balancing a concern for profitability with sustainability: (1) Governance based on a culture of shared responsibility for sustainability, (2) Sustainable and efficient use of water, (3) Biodiversity conservation, (4) Implementing a circular economy plan, (5) Technology and knowledge transfer, and (6) Image and identity. We conclude that the multi-actor transdisciplinary approach successfully facilitated the creation of a culture of shared responsibility among public, private, academic, and civil society actors. Notwithstanding plural values, challenges and solutions identified by consensus point to a nascent acknowledgement of the strategic necessity to locate agricultural economic activity within social and environmental spheres.This paper demonstrates the need to establish transdisciplinary multi-actor work-schemes to continue collaboration and research for the transition to an agro-ecological model as a means to remain competitive and to create value.
Physical Exercise Decreases the Mobile Phone Dependence of University Students in China: The Mediating Role of Self-Control
Authors: Yang Guan , Tan Guang-xin , Li Yue-xiang , Liu Hai-ying , Wang Song-tao
Published: October 2019

This study aimed to explore the relationship between physical exercise (PE) and mobile phone dependence (MPD) in Chinese university students and verify the potential role of self-control (SC) in mediating the decrease of MPD by PE. Through the quota sampling, 608 students that complied with the requirements were surveyed from 10 universities in China. PE, MPD, and SC were assessed using standard scales. For data analysis, t-tests, correlation analysis, hierarchical regression, and regression analysis were carried out in turn. The results showed significant gender differences in PE, MPD, and SC. For male students, the level of PE and score of SC were higher than those in females, yet the MPD score was lower. PE and SC were negatively related to MPD (r = −0.124, p < 0.01; r = −0.563, p < 0.001), so both could remarkably predict MPD (β = −1.00, p < 0.05; β = −0.552, p < 0.001). Gender was also a significant predictor for MPD (β = 0.089, p < 0.05). PE could, to some extent, decrease MPD, in which SC played a significant mediating role—its mediating effect accounted for nearly 71% of the total effect. The present study shows that PE is negatively correlated with MPD, and SC mediates the decrease of MPD by PE for university students in China. This indicates that the improvement of SC by PE could be a highly trustworthy and practicable way to effectively address the issue of MPD in university students or other young people across the world.
Leadership and Peer Counseling Program: Evaluation of Training and Its Impact on Filipino Senior Peer Counselors
Authors: Carandang Rogie Royce , Shibanuma Akira , Kiriya Junko , Vardeleon Karen Rose , Marges Maria Aileen , Asis Edward , Murayama Hiroshi , Jimba Masamine
Published: October 2019

Senior volunteers represented a significant, mostly untapped lay resource of informal social care. In this study, we evaluated the effectiveness of the training program on improving senior volunteers’ competency toward peer counseling, and explored its impact on their well-being after three months of the program implementation. We conducted a pre- and post-intervention analysis among 60 senior volunteers aged 60–82 years. They participated in 40 h of training and performed weekly peer counseling home visits for three months. We evaluated the program using survey questionnaires, trainer observation and debriefing, and focus group discussions. After the training, peer counselors showed a significant improvement (p < 0.001) in knowledge (17.1 versus 22.3) and skills (17.0 versus 17.9). All of them met the minimum required passing level of 70% for the knowledge test, and their overall performance was satisfactory as rated by two independent trainers. After three months, peer counselors showed a significant improvement (p < 0.001) in their subjective well-being (d = 0.69) and depressive symptoms (d = −0.67). Filipino senior volunteers could be trained to serve as peer counselors in their communities. This program improved their competency and well-being. Future research is warranted to determine whether the provision of counseling by them will affect the health outcomes of the target population.
The Mechanism of Social Organization Participation in Natural Hazards Emergency Relief: A Case Study Based on the Social Network Analysis
Authors: Chen Yingxin , Zhang Jing , Tadikamalla Pandu R. , Zhou Lei
Published: October 2019

The uncertainty and complexity of natural hazards put forward new requirements for emergency management systems. In order to deal with natural hazards effectively, it is important to build a cooperative network between government organizations and social organizations. The social network analysis method is adopted, the April 2013 Ya’an China earthquake is taken as a case study, the institutionalized emergency organization network before the disaster and the actual response organization network after the disaster are analyzed, and centrality, between centrality, closeness centrality and core-periphery are calculated. Through qualitative and quantitative research, the functions of social organization in the process of natural hazards emergency relief are revealed, the role orientation of social organization in the emergency management network is analyzed, and the influence factors of the social organization participation in the natural hazards relief is pointed out. Research results will help to promote the cooperation between social organization and government, and improve the efficiency of natural hazards emergency relief.
Economic Aspects of a Concrete Floating Offshore Wind Platform in the Atlantic Arc of Europe
Authors: Baita-Saavedra Eugenio , Cordal-Iglesias David , Filgueira-Vizoso Almudena , Castro-Santos Laura
Published: October 2019

The objective of this paper is to examine the economic aspects of a concrete offshore wind floating platform in the Atlantic Arc of Europe (Portugal and Spain). The life-cycle cost of a concrete floating offshore wind platform is considered to calculate the main economic parameters that will define the economic feasibility of the offshore wind farm. The case of study is the concrete floating offshore wind platform Telwind®, a spar platform with a revolutionary way of installing using a self-erecting telescopic tower of the wind turbine. In addition, the study analyses thirteen locations in Spain and twenty in Portugal, including the Atlantic islands of both countries. Results indicate that the economically feasible location to install a concrete offshore wind farm composed of concrete platforms is the Canary Islands (Spain) and Flores (Portugal).
Present Scenario of Bioconjugates in Cancer Therapy: A Review
Authors: Wadhawan Aishani , Chatterjee Mary , Singh Gurpal
Published: October 2019

Cancer is one of the deadliest diseases and poses a risk to people all over the world. Surgery, chemo, and radiation therapy have been the only options available until today to combat this major problem. Chemotherapeutic drugs have been used for treatment for more than 50 years. Unfortunately, these drugs have inherent cytotoxicities and tumor cells have started inducing resistance against these drugs. Other common techniques such as surgery and radiotherapy have their own drawbacks. Therefore, such techniques are incompetent tools to alleviate the disease efficiently without any adverse effects. This scenario has inspired researchers to develop alternative techniques with enhanced therapeutic effects and minimal side effects. Such techniques include targeted therapy, liposomal therapy, hormonal therapy, and immunotherapy, etc. However, these therapies are expensive and not effective enough. Furthermore, researchers have conjugated therapeutic agents or drugs with different molecules, delivery vectors, and/or imaging modalities to combat such problems and enhance the therapeutic effect. This conjugation technique has led to the development of bioconjugation therapy, in which at least one molecule is of biological origin. These bioconjugates are the new therapeutic strategies, having prospective synergistic antitumor effects and have potency to overcome the complications being produced by chemo drugs. Herein, we provide an overview of various bioconjugates developed so far, as well as their classification, characteristics, and targeting approach for cancer. Additionally, the most popular nanostructures based on their organic or inorganic origin (metallic, magnetic, polymeric nanoparticles, dendrimers, and silica nanoparticles) characterized as nanocarriers are also discussed. Moreover, we hope that this review will provide inspiration for researchers to develop better bioconjugates as therapeutic agents.
Transcription Factor GarWRKY5 Is Involved in Salt Stress Response in Diploid Cotton Species (Gossypium aridum L.)
Authors: Guo Qi , Zhao Liang , Fan Xinqi , Xu Peng , Xu Zhenzhen , Zhang Xianggui , Meng Shan , Shen Xinlian
Published: October 2019

Cotton is one of the most economically important crops in the world, and it is exposed to various abiotic stresses during its lifecycle, especially salt stress. However, the molecular mechanisms underlying cotton tolerance to salt stress are still not fully understood due to the complex nature of salt response. Therefore, identification of salt stress tolerance-related functional genes will help us understand key components involved in stress response and provide valuable genes for improving salt stress tolerance via genetic engineering in cotton. In the present study, virus-induced gene silencing of GhWRKY5 in cotton showed enhanced salt sensitivity compared to wild-type plants under salt stress. Overexpression of GarWRKY5 in Arabidopsis positively regulated salt tolerance at the stages of seed germination and vegetative growth. Additionally, GarWRKY5-overexpressing plants exhibited higher activities of superoxide dismutase (SOD) and peroxidase (POD) under salt stress. The transcriptome sequencing analysis of transgenic Arabidopsis plants and wild-type plants revealed that there was enriched coexpression of genes involved in reactive oxygen species (ROS) scavenging (including glutamine S-transferases (GSTs) and SODs) and altered response to jasmonic acid and salicylic acid in the GarWRKY5-OE lines. GarWRKY5 is involved in salt stress response by the jasmonic acid- or salicylic acid-mediated signaling pathway based on overexpression of GarWRKY5 in Arabidopsis and virus-induced gene silencing of GarWRKY5 in cotton.
Coix Seed Extract Enhances the Anti-Pancreatic Cancer Efficacy of Gemcitabine through Regulating ABCB1- and ABCG2-Mediated Drug Efflux: A Bioluminescent Pharmacokinetic and Pharmacodynamic Study
Authors: Qian Yifan , Xiong Yang , Feng Di , Wu Yali , Zhang Xu , Chen Liping , Gu Mancang
Published: October 2019

A deep insight into the function and kinetics of ATP-binding cassette (ABC) transporters may aid in the development of pharmaceutics that can minimize the particular facet of chemo-resistance. We utilized bioluminescence imaging to monitor the ABC transporter mediated intracellular drug efflux function. We also investigated the potential association between the intracellular bioluminescent pharmacokinetic profiles and the anti-tumor efficacy of the coix seed extract and gemcitabine against pancreatic cancer cells in vitro and in vivo. The bioluminescent pharmacokinetic parameters and pharmacodynamic index (IC50 and TGI) were determined. The expression levels ABCB1 and ABCG2 were assessed. Results showed that coix seed extract could synergistically enhance the anti-cancer efficacy of gemcitabine (p < 0.05). Meanwhile coix seed extract alone or in combination with gemcitabine could significantly increase the AUCluc while decreasing the Kluc (p < 0.01). Western blot and immunohistochemistry assay demonstrated that coix seed extract could significantly mitigate gemcitabine-induced upregulation of ABCB1 and ABCG2 protein. The Pearson correlation analysis demonstrated that the bioluminescent pharmacokinetic parameters and pharmacodynamic index have strong association in vitro and in vivo. In conclusion coix seed extract could augment the efficacy of gemcitabine therapy in pancreatic cancer cells may at least partly due to the alteration of ABC transporter-mediated drug efflux function.
Zinc Homeostasis in Platelet-Related Diseases
Authors: Mammadova-Bach Elmina , Braun Attila
Published: October 2019

Zn2+ deficiency in the human population is frequent in underdeveloped countries. Worldwide, approximatively 2 billion people consume Zn2+-deficient diets, accounting for 1–4% of deaths each year, mainly in infants with a compromised immune system. Depending on the severity of Zn2+ deficiency, clinical symptoms are associated with impaired wound healing, alopecia, diarrhea, poor growth, dysfunction of the immune and nervous system with congenital abnormalities and bleeding disorders. Poor nutritional Zn2+ status in patients with metastatic squamous cell carcinoma or with advanced non-Hodgkin lymphoma, was accompanied by cutaneous bleeding and platelet dysfunction. Forcing Zn2+ uptake in the gut using different nutritional supplementation of Zn2+ could ameliorate many of these pathological symptoms in humans. Feeding adult rodents with a low Zn2+ diet caused poor platelet aggregation and increased bleeding tendency, thereby attracting great scientific interest in investigating the role of Zn2+ in hemostasis. Storage protein metallothionein maintains or releases Zn2+ in the cytoplasm, and the dynamic change of this cytoplasmic Zn2+ pool is regulated by the redox status of the cell. An increase of labile Zn2+ pool can be toxic for the cells, and therefore cytoplasmic Zn2+ levels are tightly regulated by several Zn2+ transporters located on the cell surface and also on the intracellular membrane of Zn2+ storage organelles, such as secretory vesicles, endoplasmic reticulum or Golgi apparatus. Although Zn2+ is a critical cofactor for more than 2000 transcription factors and 300 enzymes, regulating cell differentiation, proliferation, and basic metabolic functions of the cells, the molecular mechanisms of Zn2+ transport and the physiological role of Zn2+ store in megakaryocyte and platelet function remain elusive. In this review, we summarize the contribution of extracellular or intracellular Zn2+ to megakaryocyte and platelet function and discuss the consequences of dysregulated Zn2+ homeostasis in platelet-related diseases by focusing on thrombosis, ischemic stroke and storage pool diseases.
Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach
Authors: Mestrom Luuk , Przypis Marta , Kowalczykiewicz Daria , Pollender André , Kumpf Antje , Marsden Stefan R. , Bento Isabel , Jarzębski Andrzej B. , Szymańska Katarzyna , Chruściel Arkadiusz , Tischler Dirk , Schoevaart Rob , Hanefeld Ulf , Hagedoorn Peter-Leon
Published: October 2019

Enzymes are nature’s catalyst of choice for the highly selective and efficient coupling of carbohydrates. Enzymatic sugar coupling is a competitive technology for industrial glycosylation reactions, since chemical synthetic routes require extensive use of laborious protection group manipulations and often lack regio- and stereoselectivity. The application of Leloir glycosyltransferases has received considerable attention in recent years and offers excellent control over the reactivity and selectivity of glycosylation reactions with unprotected carbohydrates, paving the way for previously inaccessible synthetic routes. The development of nucleotide recycling cascades has allowed for the efficient production and reuse of nucleotide sugar donors in robust one-pot multi-enzyme glycosylation cascades. In this way, large glycans and glycoconjugates with complex stereochemistry can be constructed. With recent advances, LeLoir glycosyltransferases are close to being applied industrially in multi-enzyme, programmable cascade glycosylations.
Role of Matrix Metalloproteinases 7 in the Pathogenesis of Laryngopharyngeal Reflux: Decreased E-cadherin in Acid exposed Primary Human Pharyngeal Epithelial Cells
Authors: Im Nu-Ri , Lee Doh Young , Kim Byoungjae , Kim Jian , Jung Kwang-Yoon , Kim Tae Hoon , Baek Seung-Kuk
Published: October 2019

Cleavage of E-cadherin and the resultant weakness in the cell-cell links in the laryngeal epithelium lining is induced by exposure to acidic contents of the refluxate. Herein, we aimed to evaluate the role of matrix metalloproteinases (MMPs) in inducing E-cadherin level changes following acid exposure to the human pharyngeal mucosal cells. E-cadherin levels were inversely correlated with the duration of acid exposure. Treatment with actinonin, a broad MMP inhibitor, inhibited this change. Immunocytochemical staining and transepithelial permeability test revealed that the cell surface staining of E-cadherin decreased and transepithelial permeability increased after acid exposure, which was significantly inhibited by the MMP inhibitor. Among the various MMPs analyzed, the mRNA for MMP-7 in the cellular component was upregulated, and the secretion and enzymatic activity of MMP-7 in the culture media increased with the acid treatment. Consequently, MMP-7 plays a significant role in the degradation of E-cadherin after exposure to a relatively weak acidic condition that would be similar to the physiologic condition that occurs in Laryngopharyngeal reflux disease patients.
Combination of Anti-Cancer Drugs with Molecular Chaperone Inhibitors
Authors: Shevtsov Maxim , Multhoff Gabriele , Mikhaylova Elena , Shibata Atsushi , Guzhova Irina , Margulis Boris
Published: October 2019

Most molecular chaperones belonging to heat shock protein (HSP) families are known to protect cancer cells from pathologic, environmental and pharmacological stress factors and thereby can hamper anti-cancer therapies. In this review, we present data on inhibitors of the heat shock response (particularly mediated by the chaperones HSP90, HSP70, and HSP27) either as a single treatment or in combination with currently available anti-cancer therapeutic approaches. An overview of the current literature reveals that the co-administration of chaperone inhibitors and targeting drugs results in proteotoxic stress and violates the tumor cell physiology. An optimal drug combination should simultaneously target cytoprotective mechanisms and trigger the imbalance of the tumor cell physiology.
How Oxygen Availability Affects the Antimicrobial Efficacy of Host Defense Peptides: Lessons Learned from Studying the Copper-Binding Peptides Piscidins 1 and 3
Authors: Oludiran Adenrele , Courson David S. , Stuart Malia D. , Radwan Anwar R. , Poutsma John C. , Cotten Myriam L. , Purcell Erin B.
Published: October 2019

The development of new therapeutic options against Clostridioides difficile (C. difficile) infection is a critical public health concern, as the causative bacterium is highly resistant to multiple classes of antibiotics. Antimicrobial host-defense peptides (HDPs) are highly effective at simultaneously modulating the immune system function and directly killing bacteria through membrane disruption and oxidative damage. The copper-binding HDPs piscidin 1 and piscidin 3 have previously shown potent antimicrobial activity against a number of Gram-negative and Gram-positive bacterial species but have never been investigated in an anaerobic environment. Synergy between piscidins and metal ions increases bacterial killing aerobically. Here, we performed growth inhibition and time-kill assays against C. difficile showing that both piscidins suppress proliferation of C. difficile by killing bacterial cells. Microscopy experiments show that the peptides accumulate at sites of membrane curvature. We find that both piscidins are effective against epidemic C. difficile strains that are highly resistant to other stresses. Notably, copper does not enhance piscidin activity against C. difficile. Thus, while antimicrobial activity of piscidin peptides is conserved in aerobic and anaerobic settings, the peptide–copper interaction depends on environmental oxygen to achieve its maximum potency. The development of pharmaceuticals from HDPs such as piscidin will necessitate consideration of oxygen levels in the targeted tissue.
Prolactin-Releasing Peptide: Physiological and Pharmacological Properties
Authors: Pražienková Veronika , Popelová Andrea , Kuneš Jaroslav , Maletínská Lenka
Published: October 2019

Prolactin-releasing peptide (PrRP) belongs to the large RF-amide neuropeptide family with a conserved Arg-Phe-amide motif at the C-terminus. PrRP plays a main role in the regulation of food intake and energy expenditure. This review focuses not only on the physiological functions of PrRP, but also on its pharmacological properties and the actions of its G-protein coupled receptor, GPR10. Special attention is paid to structure-activity relationship studies on PrRP and its analogs as well as to their effect on different physiological functions, mainly their anorexigenic and neuroprotective features and the regulation of the cardiovascular system, pain, and stress. Additionally, the therapeutic potential of this peptide and its analogs is explored.
Sulfolobus acidocaldarius Microvesicles Exhibit Unusually Tight Packing Properties as Revealed by Optical Spectroscopy
Authors: Bonanno Alexander , Blake Robert C. , Chong Parkson Lee-Gau
Published: October 2019

In this study, we used optical spectroscopy to characterize the physical properties of microvesicles released from the thermoacidophilic archaeon Sulfolobus acidocaldarius (Sa-MVs). The most abundant proteins in Sa-MVs are the S-layer proteins, which self-assemble on the vesicle surface forming an array of crystalline structures. Lipids in Sa-MVs are exclusively bipolar tetraethers. We found that when excited at 275 nm, intrinsic protein fluorescence of Sa-MVs at 23 °C has an emission maximum at 303 nm (or 296 nm measured at 75 °C), which is unusually low for protein samples containing multiple tryptophans and tyrosines. In the presence of 10–11 mM of the surfactant n-tetradecyl-β-d-maltoside (TDM), Sa-MVs were disintegrated, the emission maximum of intrinsic protein fluorescence was shifted to 312 nm, and the excitation maximum was changed from 288 nm to 280.5 nm, in conjunction with a significant decrease (>2 times) in excitation band sharpness. These data suggest that most of the fluorescent amino acid residues in native Sa-MVs are in a tightly packed protein matrix and that the S-layer proteins may form J-aggregates. The membranes in Sa-MVs, as well as those of unilamellar vesicles (LUVs) made of the polar lipid fraction E (PLFE) tetraether lipids isolated from S. acidocaldarius (LUVPLFE), LUVs reconstituted from the tetraether lipids extracted from Sa-MVs (LUVMV) and LUVs made of the diester lipids, were investigated using the probe 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan). The generalized polarization (GP) values of Laurdan in tightly packed Sa-MVs, LUVMV, and LUVPLFE were found to be much lower than those obtained from less tightly packed DPPC gel state, which echoes the previous finding that the GP values from tetraether lipid membranes cannot be directly compared with the GP values from diester lipid membranes, due to differences in probe disposition. Laurdan’s GP and red-edge excitation shift (REES) values in Sa-MVs and LUVMV decrease with increasing temperature monotonically with no sign for lipid phase transition. Laurdan’s REES values are high (9.3–18.9 nm) in the tetraether lipid membrane systems (i.e., Sa-MVs, LUVMV and LUVPLFE) and low (0.4–5.0 nm) in diester liposomes. The high REES and low GP values suggest that Laurdan in tetraether lipid membranes, especially in the membrane of Sa-MVs, is in a very motionally restricted environment, bound water molecules and the polar moieties in the tetraether lipid headgroups strongly interact with Laurdan’s excited state dipole moment, and “solvent” reorientation around Laurdan’s chromophore in tetraether lipid membranes occurs very slowly compared to Laurdan’s lifetime.
Autophagy Modulation in Human Thyroid Cancer Cells following Aloperine Treatment
Authors: Yu Hui-I , Shen Hui-Ching , Chen Shu-Hsin , Lim Yun-Ping , Chuang Hsiang-Hsun , Tai Tsai-Sung , Kung Fang-Ping , Lu Chieh-Hsiang , Hou Chia-Yi , Lee Ying-Ray
Published: October 2019

Aloperine, an alkaloid isolated from Sophora alopecuroides, exhibits multiple pharmacological activities including anti-inflammatory, antioxidant, antiallergic, antinociceptive, antipathogenic, and antitumor effects. Furthermore, it exerts protective effects against renal and neuronal injuries. Several studies have reported antitumor effects of aloperine against various human cancers, including multiple myeloma; colon, breast, and prostate cancers; and osteosarcoma. Cell cycle arrest, apoptosis induction, and tumorigenesis suppression have been demonstrated following aloperine treatment. In a previous study, we demonstrated antitumor effects of aloperine on human thyroid cancer cells through anti-tumorigenesis and caspase-dependent apoptosis induction via the Akt signaling pathway. In the present study, we demonstrated the modulation of the autophagy mechanism following the incubation of multidrug-resistant papillary and anaplastic human thyroid cancer cells with aloperine; we also illustrate the underlying mechanisms, including AMPK, Erk, JNK, p38, and Akt signaling pathways. Further investigation revealed the involvement of the Akt signaling pathway in aloperine-modulated autophagy in human thyroid cancer cells. These results indicate a previously unappreciated function of aloperine in autophagy modulation in human thyroid cancer cells.
Reaction of Dialumane Incorporating Bulky Eind Groups with Pyridines
Authors: Murosaki Takahiro , Ohno Ryoma , Agou Tomohiro , Hashizume Daisuke , Matsuo Tsukasa
Published: October 2019

The reaction of the bulky Eind-based dialumane, (Eind)HAl(μ-H)2AlH(Eind) (1) (Eind = 1,1,3,3,5,5,7,7-octaethyl-s-hydrindacen-4-yl), with pyridines is described. When 1 was treated with pyridine (Py) in toluene, the Py adduct of aryldihydroalumane, Py→AlH2(Eind) (2), was initially formed. Then, the hydroalumination of Py took place to yield the Py-bound aryl(1,4-dihydropyrid-1-yl)hydroalumane, Py→AlH(1,4-dihydropyrid-1-yl)(Eind) (3). A similar reaction with a stronger Lewis base, 4-pyrrolidinopyridine (PPy), produced the stable PPy adduct, PPy→AlH2(Eind) (4). The resulting organoaluminum compounds have been fully characterized by NMR spectroscopy as well as X-ray crystallography. The reaction mechanism from 1 to 3 via 2 has been examined by deuterium labeling experiments using (Eind)DAl(μ-D)2AlD(Eind) (1-d4).
Insecticidal and Antifeedant Activities of Malagasy Medicinal Plant (Cinnamosma sp.) Extracts and Drimane-Type Sesquiterpenes against Aedes aegypti Mosquitoes
Authors: Inocente Edna Alfaro , Nguyen Bao , Manwill Preston K. , Benatrehina Annecie , Kweka Eliningaya , Wu Sijin , Cheng Xiaolin , Rakotondraibe L. Harinantenaina , Piermarini Peter M.
Published: October 2019

The overuse of insecticides with limited modes of action has led to resistance in mosquito vectors. Thus, insecticides with novel modes of action are needed. Secondary metabolites in Madagascan plants of the genus Cinnamosma (Canellaceae) are commonly used in traditional remedies and known to elicit antifeedant and toxic effects in insect pests. Here we test the hypothesis that extracts of Cinnamosma sp. enriched in drimane sesquiterpenes are toxic and/or antifeedant to the yellow fever mosquito Aedes aegypti. We show that the bark and root extracts, which contain a higher abundance of drimane sesquiterpenes compared to leaves, were the most efficacious. Screening isolated compounds revealed cinnamodial to be the primary driver of adulticidal activity, whereas cinnamodial, polygodial, cinnafragrin A, and capsicodendrin contributed to the larvicidal activity. Moreover, an abundant lactone (cinnamosmolide) in the root extract synergized the larvicidal effects of cinnamodial. The antifeedant activity of the extracts was primarily contributed to cinnamodial, polygodial, and cinnamolide. Parallel experiments with warburganal isolated from Warburgia ugandensis (Canellaceae) revealed that aldehydes are critical for—and a hydroxyl modulates—insecticidal activity. Our results indicate that plant drimane sesquiterpenes provide valuable chemical platforms for developing insecticides and repellents to control mosquito vectors.
Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging
Authors: Zhang Qimei , Grabowska Anna M. , Clarke Philip A. , Morgan Stephen P.
Published: October 2019

The spatial resolution and light detected in fluorescence imaging for small animals are limited by light scattering, absorption and autofluorescence. To address this, novel near-infrared fluorescent contrast agents and imaging configurations have been investigated. In this paper, the influence of the light wavelength and imaging configurations (full-field illumination system and scanning system) on fluorescence imaging are compared quantitatively. The surface radiance for both systems is calculated by modifying the simulation tool Near-Infrared Fluorescence and Spectral Tomography. Fluorescent targets are embedded within a scattering medium at different positions. The surface radiance and spatial resolution are obtained for emission wavelengths between 620 nm and 1000 nm. It was found that the spatial resolution of the scanning system is independent of the tissue optical properties, whereas for full-field illumination, the spatial resolution degrades at longer wavelength. The full width at half maximum obtained by the scanning system is 25% lower than that obtained by the full-field illumination system when the targets are located in the middle of the phantom. The results indicate that although imaging at near-infrared wavelength can achieve a higher surface radiance, it may produce worse spatial resolution.
The Future of Hyperspectral Imaging
Authors: Selci Stefano
Published: October 2019

The Special Issue on hyperspectral imaging (HSI), entitled “The Future of Hyperspectral Imaging”, has published 12 papers. Nine papers are related to specific current research and three more are review contributions: In both cases, the request is to propose those methods or instruments so as to show the future trends of HSI. Some contributions also update specific methodological or mathematical tools. In particular, the review papers address deep learning methods for HSI analysis, while HSI data compression is reviewed by using liquid crystals spectral multiplexing as well as DMD-based Raman spectroscopy. Specific topics explored by using data obtained by HSI include alert on the sprouting of potato tubers, the investigation on the stability of painting samples, the prediction of healing diabetic foot ulcers, and age determination of blood-stained fingerprints. Papers showing advances on more general topics include video approach for HSI dynamic scenes, localization of plant diseases, new methods for the lossless compression of HSI data, the fusing of multiple multiband images, and mixed modes of laser HSI imaging for sorting and quality controls.
Ongoing Challenges with Healthcare-Associated Candida auris Outbreaks in Oman
Authors: Al Maani Amal , Paul Hema , Al-Rashdi Azza , Al Wahaibi Adil , Al-Jardani Amina , Al Abri Asma M. Ali , AlBalushi Mariam A. H. , Al Abri Seif , Al Reesi Mohammed , Al Maqbali Ali , Al Kasaby Nashwa M. , de Groot Theun , F. Meis Jacques , Al-Hatmi Abdullah M. S.
Published: October 2019

Candida auris has emerged in the past decade as a multi-drug resistant public health threat causing health care outbreaks. Here we report epidemiological, clinical, and microbiological investigations of a C. auris outbreak in a regional Omani hospital between April 2018 and April 2019. The outbreak started in the intensive care areas (intensive care unit (ICU), coronary care unit (CCU), and high dependency unit) but cases were subsequently diagnosed in other medical and surgical units. In addition to the patients’ clinical and screening samples, environmental swabs from high touch areas and from the hands of 35 staff were collected. All the positive samples from patients and environmental screening were confirmed using MALDI-TOF, and additional ITS-rDNA sequencing was done for ten clinical and two environmental isolates. There were 32 patients positive for C. auris of which 14 (43.8%) had urinary tract infection, 11 (34.4%) had candidemia, and 7 (21.8%) had asymptomatic skin colonization. The median age was 64 years (14–88) with 17 (53.1%) male and 15 (46.9%) female patients. Prior to diagnosis, 21 (65.6%) had been admitted to the intensive care unit, and 11 (34.4%) had been nursed in medical or surgical wards. The crude mortality rate in our patient’s cohort was 53.1. Two swabs collected from a ventilator in two different beds in the ICU were positive for C. auris. None of the health care worker samples were positive. Molecular typing showed that clinical and environmental isolates were genetically similar and all belonged to the South Asian C. auris clade I. Most isolates had non-susceptible fluconazole (100%) and amphotericin B (33%) minimal inhibitory concentrations (MICs), but had low echinocandin and voriconazole MICs. Despite multimodal infection prevention and control measures, new cases continued to appear, challenging all the containment efforts.
Multiscale Modeling for Application-Oriented Optimization of Resistive Random-Access Memory
Authors: La Torraca Paolo , Puglisi Francesco Maria , Padovani Andrea , Larcher Luca
Published: October 2019

Memristor-based neuromorphic systems have been proposed as a promising alternative to von Neumann computing architectures, which are currently challenged by the ever-increasing computational power required by modern artificial intelligence (AI) algorithms. The design and optimization of memristive devices for specific AI applications is thus of paramount importance, but still extremely complex, as many different physical mechanisms and their interactions have to be accounted for, which are, in many cases, not fully understood. The high complexity of the physical mechanisms involved and their partial comprehension are currently hampering the development of memristive devices and preventing their optimization. In this work, we tackle the application-oriented optimization of Resistive Random-Access Memory (RRAM) devices using a multiscale modeling platform. The considered platform includes all the involved physical mechanisms (i.e., charge transport and trapping, and ion generation, diffusion, and recombination) and accounts for the 3D electric and temperature field in the device. Thanks to its multiscale nature, the modeling platform allows RRAM devices to be simulated and the microscopic physical mechanisms involved to be investigated, the device performance to be connected to the material’s microscopic properties and geometries, the device electrical characteristics to be predicted, the effect of the forming conditions (i.e., temperature, compliance current, and voltage stress) on the device’s performance and variability to be evaluated, the analog resistance switching to be optimized, and the device’s reliability and failure causes to be investigated. The discussion of the presented simulation results provides useful insights for supporting the application-oriented optimization of RRAM technology according to specific AI applications, for the implementation of either non-volatile memories, deep neural networks, or spiking neural networks.
Flame Spraying of Aluminum Coatings Reinforced with Particles of Carbonaceous Materials as an Alternative for Laser Cladding Technologies
Authors: Czupryński Artur
Published: October 2019

The article presents results of the preliminary research of mechanical properties of flame-sprayed aluminum coatings reinforced with carbon materials made on the construction steel S235J0 substrate. For reinforcement the following carbon materials were used: carbon nanotubes Nanocyl NC 7000 (0.5 wt.% and 1 wt.%) and carburite (0.5 wt.%). The properties evaluation was made using metallographic macroscope and microscope, chemical composition, microhardness, abrasion and erosion resistance studies. The obtained results were compared with aluminum powder coatings (EN AW 1000 series). It was proved that the flame spraying of aluminum coatings reinforced with particles of carbonaceous materials can be an effective alternative for laser cladding technology. The preliminary test results will be successively extended by further experiments to contribute in the near future to develop innovative technologies, that can be implemented in the automotive industry for production of components with high strength, wear resistance, good thermal conductivity and low density, such as brake shoes, cylinder liners, piston rings and gears.
Ohmic Contacts on p-Type Al-Implanted 4H-SiC Layers after Different Post-Implantation Annealings
Authors: Spera Monia , Greco Giuseppe , Corso Domenico , Di Franco Salvatore , Severino Andrea , Messina Angelo Alberto , Giannazzo Filippo , Roccaforte Fabrizio
Published: October 2019

This paper reports on the electrical activation and Ohmic contact properties on p-type Al-implanted silicon carbide (4H-SiC). In particular, the contacts were formed on 4H-SiC-implanted layers, subjected to three different post-implantation annealing processes, at 1675 °C, 1175 °C, and 1825 °C. Under these post-implantation annealing conditions, the electrical activation of the Al dopant species increased from 39% to 56%. The Ti/Al/Ni contacts showed an Ohmic behavior after annealing at 950 °C. The specific contact resistance ρc could be lowered by a factor of 2.6 with the increase of the post-implantation annealing temperature. The result can be useful for application in device fabrication. Moreover, the dependence of ρc on the active acceptor concentration followed the thermionic field emission model, with a barrier height of 0.63 eV.
Residual Flexural Performance of Epoxy Polymer Concrete under Hygrothermal Conditions and Ultraviolet Aging
Authors: Ma Dongpeng , Pan Zhiwei , Liu Yiping , Jiang Zhenyu , Liu Zejia , Zhou Licheng , Tang Liqun
Published: October 2019

Epoxy polymer concrete (EPC) has found increasing applications in infrastructure as a rising candidate among civil engineering materials. In most of its service environments, EPC is inevitably exposed to severe weather conditions, e.g., violent changes in temperature, rain, and ultraviolet (UV) radiation. In this paper, we designed an accelerated aging test for EPC, which includes periodic variation of temperature and water spray, as well as intensive UV-light irradiation, imitating the outdoor environment in South China. The experimental results show that the flexural performance of EPC is found deteriorate with the aging time. An aging process equivalent to four years (UV radiation dose) results in up to 8.4% reduction of flexural strength. To explore the mechanisms of observed performance degradation, the EPC specimen in the four-point-bending test is considered as a layered beam. The analysis indicates that the loss of flexural load-carrying capacity of an aged EPC beam is dominated by the reduction of mechanical properties of the surface layer. The mechanical properties of the surface layer are closely associated with the aging of epoxy mortar, which can be approximated as a reciprocal function of the aging time. By introducing damage to the surface layer into the layered beam, the proposed model demonstrates a good ability to predict the residual flexural strength of EPC during the aging process
Neutral Axis Depth versus Ductility and Plastic Rotation Capacity on Bending in Lightweight-Aggregate Concrete Beams
Authors: Bernardo Luís , Nepomuceno Miguel , Pinto Hugo
Published: October 2019

This article presents an experimental study on the evolution of the neutral axis depth at failure in the critical section with the flexural ductility and plastic rotation capacity of reinforced concrete (RC) lightweight-aggregate concrete (LWAC) beams. For this, the results of a previous experimental program involving RC LWAC beams tested in flexure until failure are used. The variable studies were the concrete compressive strength (between 22.0 and 60.4 MPa and dry density between 1651 and 1953 kg/m3) and the longitudinal tensile reinforcement ratio (between 0.13% and 2.69%). The flexural ductility and the plastic rotation capacity of the RC LWAC beams are characterized by a ductility index and a plastic trend parameter, respectively. The influence of the variable studies, as well as the relation of the flexural ductility and plastic rotation capacity with the values for the neutral axis depth at failure are analyzed and discussed. Some conclusions are drawn which can be useful for the design of RC LWAC beams for flexure. In particular, it is shown that the practical rule of limiting the neutral axis depth at failure to ensure ductile behavior, as used in normal-weight aggregate concrete beams, is also valid for RC LWAC beams.
A Superhydrophilic Aluminum Surface with Fast Water Evaporation Based on Anodic Alumina Bundle Structures via Anodizing in Pyrophosphoric Acid
Authors: Nakajima Daiki , Kikuchi Tatsuya , Yoshioka Taiki , Matsushima Hisayoshi , Ueda Mikito , Suzuki Ryosuke O. , Natsui Shungo
Published: October 2019

A superhydrophilic aluminum surface with fast water evaporation based on nanostructured aluminum oxide was fabricated via anodizing in pyrophosphoric acid. Anodizing aluminum in pyrophosphoric acid caused the successive formation of a barrier oxide film, a porous oxide film, pyramidal bundle structures with alumina nanofibers, and completely bent nanofibers. During the water contact angle measurements at 1 s after the water droplet was placed on the anodized surface, the contact angle rapidly decreased to less than 10°, and superhydrophilic behavior with the lowest contact angle measuring 2.0° was exhibited on the surface covered with the pyramidal bundle structures. As the measurement time of the contact angle decreased to 200–33 ms after the water placement, although the contact angle slightly increased in the initial stage due to the formation of porous alumina, at 33 ms after the water placement, the contact angle was 9.8°, indicating that superhydrophilicity with fast water evaporation was successfully obtained on the surface covered with the pyramidal bundle structures. We found that the shape of the pyramidal bundle structures was maintained in water without separation by in situ high-speed atomic force microscopy measurements.