bluuebunny/crossref_metadata_2025 huggingface dataset:
Future Drug Targets in Periodontal Personalised Medicine—A Narrative Review
Authors: Yadalam Pradeep Kumar , Kalaivani V. , Fageeh Hammam Ibrahim , Ibraheem Wael , Al-Ahmari Manea Musa. , Khan Samar Saeed , Ahmed Zeeshan Heera , Abdulkarim Hesham H. , Baeshen Hosam Ali , Balaji Thodur Madapusi , Bhandi Shilpa , Raj A. Thirumal , Patil Shankargouda
Published: March 2022

Periodontal disease is an infection-driven inflammatory disease characterized by the destruction of tooth-supporting tissues. The establishment of chronic inflammation will result in progressive destruction of bone and soft tissue changes. Severe periodontitis can lead to tooth loss. The disease has complex pathogenesis with an interplay between genetic, environmental, and host factors and pathogens. Effective management consists of plaque control and non-surgical interventions, along with adjuvant strategies to control inflammation and disrupt the pathogenic subgingival biofilms. Recent studies have examined novel approaches for managing periodontal diseases such as modulating microbial signaling mechanisms, tissue engineering, and molecular targeting of host inflammatory substances. Mounting evidence suggests the need to integrate omics-based approaches with traditional therapy to address the disease. This article discusses the various evolving and future drug targets, including proteomics, gene therapeutics, vaccines, and nanotechnology in personalized periodontal medicine for the effective management of periodontal diseases.
A Handcrafted Radiomics-Based Model for the Diagnosis of Usual Interstitial Pneumonia in Patients with Idiopathic Pulmonary Fibrosis
Authors: Refaee Turkey , Bondue Benjamin , Van Simaeys Gaetan , Wu Guangyao , Yan Chenggong , Woodruff Henry , Goldman Serge , Lambin Philippe
Published: March 2022

The most common idiopathic interstitial lung disease (ILD) is idiopathic pulmonary fibrosis (IPF). It can be identified by the presence of usual interstitial pneumonia (UIP) via high-resolution computed tomography (HRCT) or with the use of a lung biopsy. We hypothesized that a CT-based approach using handcrafted radiomics might be able to identify IPF patients with a radiological or histological UIP pattern from those with an ILD or normal lungs. A total of 328 patients from one center and two databases participated in this study. Each participant had their lungs automatically contoured and sectorized. The best radiomic features were selected for the random forest classifier and performance was assessed using the area under the receiver operator characteristics curve (AUC). A significant difference in the volume of the trachea was seen between a normal state, IPF, and non-IPF ILD. Between normal and fibrotic lungs, the AUC of the classification model was 1.0 in validation. When classifying between IPF with a typical HRCT UIP pattern and non-IPF ILD the AUC was 0.96 in validation. When classifying between IPF with UIP (radiological or biopsy-proved) and non-IPF ILD, an AUC of 0.66 was achieved in the testing dataset. Classification between normal, IPF/UIP, and other ILDs using radiomics could help discriminate between different types of ILDs via HRCT, which are hardly recognizable with visual assessments. Radiomic features could become a valuable tool for computer-aided decision-making in imaging, and reduce the need for unnecessary biopsies.
Inhibition of Ceramide Glycosylation Enhances Cisplatin Sensitivity in Cholangiocarcinoma by Limiting the Activation of the ERK Signaling Pathway
Authors: Chueakwon Piyasiri , Jatooratthawichot Peeranat , Talabnin Krajang , Ketudat Cairns James R. , Talabnin Chutima
Published: March 2022

Cholangiocarcinoma (CCA) is an aggressive tumor of the biliary epithelium with poor survival that shows limited response to conventional chemotherapy. Increased expression of glucosylceramide synthase (GCS) contributes to drug resistance and the progression of various cancers; the expression profiles of GCS (UGCG) and the genes for glucocerebrosidases 1, 2, and 3 (GBA1, GBA2, and GBA3) were therefore studied in CCA. The biological functions of GCS for cell proliferation and cisplatin sensitivity in CCA were explored. GCS expression was higher in CCA tumor tissues than that of GBA1, GBA2, and GBA3. Verification of GCS expression in 29 paired frozen CCA tissues showed that 8 of 29 cases (27.6%) had high GCS expression. The expression of GCS and GBA2 was induced in CCA cell lines following low-dose cisplatin treatment. Suppression of GCS by either palmitoylamino-3-morpholino-1-propanol (PPMP), GCS knockdown or a combination of the two resulted in reduced cell proliferation. These treatments enhanced the effect of cisplatin-induced CCA cell death, increased the expression of apoptotic proteins and reduced phosphorylation of ERK upon cisplatin treatment. Taken together, inhibition of the GCS increased cisplatin-induced CCA apoptosis via the inhibition of the ERK signaling pathway. Thus, targeting GCS might be a strategy for CCA treatment.
Taurine Ameliorates Tunicamycin-Induced Liver Injury by Disrupting the Vicious Cycle between Oxidative Stress and Endoplasmic Reticulum Stress
Authors: Kim Sou Hyun , Seo Hyeji , Kwon Doyoung , Yuk Dong Yeon , Jung Young-Suk
Published: March 2022

Non-alcoholic fatty liver disease (NAFLD) is a chronic liver dysfunction characterized by excess lipid accumulation; non-alcoholic steatohepatitis can transform into more severe conditions, such as cirrhosis and hepatocellular carcinoma. Although several pharmacologic approaches have been evaluated in clinical trials, there are no approved therapies for NAFLD. Previous studies have suggested that taurine supplementation alleviates fatty liver; however, the underlying mechanism remains obscure. In this study, we investigated the beneficial effects of taurine on fatty liver injury in vivo induced by tunicamycin, a chemical endoplasmic reticulum (ER) stressor. The mice were administered 2% taurine for 2 weeks prior to intraperitoneal tunicamycin injection; after 72 h of treatment, the mice were euthanized. Tunicamycin treatment significantly increased the levels of serum ALT and AST and hepatic triglycerides. Notably, these changes were alleviated by taurine supplementation. Taurine normalized the protein and/or mRNA levels involved in ER stress signaling (IRE1a, p-IRE1a, ATF6, XBP1, BiP, and CHOP) and lipid metabolism (CD36, MTTP, and ApoB), which were dysregulated by tunicamycin treatment. The stimulation of hepatic lipid export by taurine was evidenced by the recovery of blood VLDL levels. Furthermore, taurine supplementation prevented tunicamycin-induced lipid peroxidation and decreased glutathione (GSH) levels by correcting abnormal cysteine catabolism involved in the production of both taurine and GSH. Therefore, taurine supplementation can prevent tunicamycin-induced liver injury by counteracting oxidative and ER stress.
Decision-Making of Transnational Supply Chain Considering Tariff and Third-Party Logistics Service
Authors: Hu Xiao , Fu Kaifang , Chen Zhixiang , Du Zhijiao
Published: March 2022

Countries’ economic policies, such as tariff barriers, have a profound impact on the global economy and international trade. The imposition of tariffs seriously disturbs the global trade and supply chain operations. This paper studies a supply chain composed of an overseas manufacturer, a domestic supplier and a third-party integrated international logistics service provider. A three-level decentralized leader-follower decision-making model and its variant--leader-follower alliance decision-making models are established, and the influences of revenue sharing and cost sharing on the three-level decentralized decision-making are analyzed. The results show that it is difficult for the supply chain to achieve coordination when the transportation and insurance costs are considered in the tariff cost. The increase of tariff rates will reduce the profits of all parties and the overall profit of the supply chain, and weaken the dominant position of the supplier in the supply chain. Revenue sharing can improve the supply chain performance; the performance of the whole supply chain cannot be improved or may even deteriorate by sharing the transportation cost alone. The study can provide practitioners with implications for how to carry effective cooperation and coordination in the supply chain and how to effectively reduce the influence of tariffs in the global trade system.
Infinite–Dimensional Bifurcations in Spatially Distributed Delay Logistic Equation
Authors: Kashchenko Sergey
Published: March 2022

This paper investigates the questions about the local dynamics in the neighborhood of the equilibrium state for the spatially distributed delay logistic equation with diffusion. The critical cases in the stability problem are singled out. The equations for their invariant manifolds that determine the structure of the solutions in the equilibrium state neighborhood are constructed. The dominant bulk of this paper is devoted to the consideration of the most interesting and important cases of either the translation (advection) coefficient is large enough or the diffusion coefficient is small enough. Both of this cases convert the original problem to a singularly perturbed one. It is shown that under these conditions the critical cases are infinite–dimensional in the problems of the equilibrium state stability for the singularly perturbed problems. This means that infinitely many roots of the characteristic equations of the corresponding linearized boundary value problems tend to the imaginary axis as the small parameter tends to zero. Thus, we are talking about infinite–dimensional bifurcations. Standard approaches to the study of the local dynamics based on the application of the invariant integral manifolds methods and normal forms methods are not applicable. Therefore, special methods of infinite–dimensional normalization have been developed which allow one to construct special nonlinear boundary value problems called quasinormal forms. Their nonlocal dynamics determine the behavior of the initial boundary value problem solutions in the neighborhood of the equilibrium state. The bifurcation features arising in the case of different boundary conditions are illustrated.
Super Resolution for Noisy Images Using Convolutional Neural Networks
Authors: Mushtaq Zaid , Nasti Shoaib , Verma Chaman , Raboaca Maria , Kumar Neerendra , Nasti Samiah
Published: March 2022

The images in high resolution contain more useful information than the images in low resolution. Thus, high-resolution digital images are preferred over low-resolution images. Image super-resolution is one of the principal techniques for generating high-resolution images. The major advantages of super-resolution methods are that they are economical, independent of the image capture devices, and can be statically used. In this paper, a single-image super-resolution network model based on convolutional neural networks is proposed by combining conventional autoencoder and residual neural network approaches. A convolutional neural network-based dictionary method is used to train low-resolution input images for high-resolution images. In addition, a linear refined unit thresholds the convolutional neural network output to provide a better low-resolution image dictionary. Autoencoders aid in the removal of noise from images and the enhancement of their quality. Secondly, the residual neural network model processes it further to create a high-resolution image. The experimental results demonstrate the outstanding performance of our proposed method compared to other traditional methods. The proposed method produces clearer and more detailed high-resolution images, as they are important in real-life applications. Moreover, it has the advantage of combining convolutional neural network-based dictionary learning, autoencoder image enhancement, and noise removal. Furthermore, residual neural network training with improved preprocessing creates an efficient and versatile single-image super-resolution network.
Rings of Multisets and Integer Multinumbers
Authors: Chopyuk Yuriy , Vasylyshyn Taras , Zagorodnyuk Andriy
Published: March 2022

In the paper, we consider a ring structure on the Cartesian product of two sets of integer multisets. In this way, we introduce a ring of integer multinumbers as a quotient of the Cartesian product with respect to a natural equivalence. We examine the properties of this ring and construct some isomorphisms to subrings of polynomials and Dirichlet series with integer coefficients. In addition, we introduce finite rings of multinumbers “modulo (p,q)” and propose an algorithm for construction of invertible elements in these rings that may be applicable in Public-key Cryptography. An analog of the Little Fermat Theorem for integer multinumbers is proved.
Rational Approximations in Robust Preconditioning of Multiphysics Problems
Authors: Harizanov Stanislav , Lirkov Ivan , Margenov Svetozar
Published: March 2022

Multiphysics or multiscale problems naturally involve coupling at interfaces which are manifolds of lower dimensions. The block-diagonal preconditioning of the related saddle-point systems is among the most efficient approaches for numerically solving large-scale problems in this class. At the operator level, the interface blocks of the preconditioners are fractional Laplacians. At the discrete level, we propose to replace the inverse of the fractional Laplacian with its best uniform rational approximation (BURA). The goal of the paper is to develop a unified framework for analysis of the new class of preconditioned iterative methods. As a final result, we prove that the proposed preconditioners have optimal computational complexity O(N), where N is the number of unknowns (degrees of freedom) of the coupled discrete problem. The main theoretical contribution is the condition number estimates of the BURA-based preconditioners. It is important to note that the obtained estimates are completely analogous for both positive and negative fractional powers. At the end, the analysis of the behavior of the relative condition numbers is aimed at characterizing the practical requirements for minimal BURA orders for the considered Darcy–Stokes and 3D–1D examples of coupled problems.
Photoreaction Pathways of Bacteriorhodopsin and Its D96N Mutant as Revealed by in Situ Photoirradiation Solid-State NMR
Authors: Shigeta Arisu , Otani Yuto , Miyasa Ryota , Makino Yoshiteru , Kawamura Izuru , Okitsu Takashi , Wada Akimori , Naito Akira
Published: March 2022

Bacteriorhodopsin (BR) functions as a light-driven proton pump that transitions between different states during the photocycle, such as all-trans (AT; BR568) and 13-cis, 15-syn (CS; BR548) state and K, L, M1, M2, N, and O intermediates. In this study, we used in situ photoirradiation 13C solid-state NMR to observe a variety of photo-intermediates and photoreaction pathways in [20-13C]retinal-WT-BR and its mutant [20-13C, 14-13C]retinal-D96N-BR. In WT-BR, the CS state converted to the CS* intermediate under photoirradiation with green light at −20 °C and consequently converted to the AT state in the dark. The AT state converted to the N intermediate under irradiation with green light. In D96N-BR, the CS state was converted to the CS* intermediate at −30 °C and consequently converted to the AT state. Simultaneously, the AT state converted to the M and L intermediates under green light illumination at −30 °C and subsequently converted to the AT state in the dark. The M intermediate was directly excited to the AT state by UV light illumination. We demonstrated that short-lived photo-intermediates could be observed in a stationary state using in situ photoirradiation solid-state NMR spectroscopy for WT-BR and D96N-BR, enabling insight into the light-driven proton pump activity of BR.
Studies of Circuit Design, Structural, Relaxation and Potential Stability of Polymer Blend Electrolyte Membranes Based on PVA:MC Impregnated with NH4I Salt
Authors: Nofal Muaffaq M. , Aziz Shujahadeen B. , Brza Mohamad A. , Abdullah Sozan N. , Dannoun Elham M. A. , Hadi Jihad M. , Murad Ary R. , Al-Saeedi Sameerah I. , Kadir Mohd F. Z.
Published: March 2022

This work presents the fabrication of polymer electrolyte membranes (PEMs) that are made of polyvinyl alcohol-methylcellulose (PVA-MC) doped with various amounts of ammonium iodide (NH4I). The structural and electrical properties of the polymer blend electrolyte were performed via the acquisition of Fourier Transform Infrared (FTIR) and electrical impedance spectroscopy (EIS), respectively. The interaction among the components of the electrolyte was confirmed via the FTIR approach. Electrical impedance spectroscopy (EIS) showed that the whole conductivity of complexes of PVA-MC was increased beyond the addition of NH4I. The application of EEC modeling on experimental data of EIS was helpful to calculate the ion transport parameters and detect the circuit elements of the films. The sample containing 40 wt.% of NH4I salt exhibited maximum ionic conductivity (7.01 × 10−8) S cm−1 at room temperature. The conductivity behaviors were further emphasized from the dielectric study. The dielectric constant, ε’ and loss, ε’’ values were recorded at high values within the low-frequency region. The peak appearance of the dielectric relaxation analysis verified the non-Debye type of relaxation mechanism was clarified via the peak appearance of the dielectric relaxation. For further confirmation, the transference number measurement (TNM) of the PVA-MC-NH4I electrolyte was analyzed in which ions were primarily entities for the charge transfer process. The linear sweep voltammetry (LSV) shows a relatively electrochemically stable electrolyte where the voltage was swept linearly up to 1.6 V. Finally, the sample with maximum conductivity, ion dominance of tion and relatively wide breakdown voltage were found to be 0.88 and 1.6 V, respectively. As the ions are the majority charge carrier, this polymer electrolyte could be considered as a promising candidate to be used in electrochemical energy storage devices for example electrochemical double-layer capacitor (EDLC) device.
Enhanced Wear Resistance of the Ultrastrong Ultrasonic Shot-Peened M50 Bearing Steel with Gradient Nanograins
Authors: Dong Zhaohua , Wang Feng , Qian Dongsheng , Yin Fei , Wang Huiling , Wang Xiaokang , Hu Shan , Chi Jie
Published: March 2022

The enhancement of the wear resistance has long been important for bearing steels, hampered in part by the lack of suitable surface strengthening techniques. In this study, ultrasonic shot peening (USP) is employed to enhance the wear resistance of the M50 bearing steel by generating an ultrastrong surface layer with gradient nanograins. The effects of USP treatment on the surface integrity, microstructures, and wear behavior of M50 steel are investigated. The microstructural observation shows that the gradient nanograined surface layer is over 200 μm thick. The lath martensite is refined to nano-martensitic subgrains with few low angle boundaries in the USP-treated M50 steel. Additionally, the microhardness of the nanostructured M50 steel is increased from 795 HV to 987 HV with a penetrating depth of around 800 μm. Wear tests show that the wear rate of the USP-treated M50 steel is reduced by 50.4% under sliding conditions compared to that of the untreated samples. The significantly enhanced wear resistance of the USP-treated M50 is attributed to the ultrastrong gradient nanograined surface layer. The wear mechanism of the USP-treated M50 steel changes from oxidative wear and severe plowing wear to mild plowing wear. Additionally, the thickness of the plastic deformation layer generated during the friction process is reduced. This work provides the materials scientists and engineers with an efficient surface treatment method to enhance the wear resistance of high-strength steels.
Effect of Solution Temperature on the Microstructure and Properties of 17-4PH High-Strength Steel Samples Formed by Selective Laser Melting
Authors: Zhao Zhanyong , Wang Hailin , Huo Pengcheng , Bai Peikang , Du Wenbo , Li Xiaofeng , Li Jianmin , Zhang Wei
Published: March 2022

Samples of 17-4PH high-strength steel were processed by selective laser melting (SLM) and solution-processed. The effects of the solution temperature on the microstructure and mechanical properties of the samples were studied. The 17-4PH high-strength steel is primarily composed of martensite, with a small number of austenite phases, and contains many dislocations. After the solution treatment, the grain size gradually increased, yielding typical martensite. The samples were subjected to an aging treatment after the solution treatment. Precipitates formed in the samples, conducive to improving their strength and hardness. The Vickers hardening and wear properties of the 17-4PH high-strength steel samples first increased and then decreased with increasing solution temperature. After the solution treatment at 1040 °C for 2 h and aging at 480 °C for 4 h, the Vickers hardening of the 17-4PH high-strength steel increased to 392 HV0.5, and the friction coefficient was approximately 0.6. These values were, respectively, 7% and 5% higher than those for the untreated samples.
Strain-Controlled Fatigue Behavior of a Nodular Cast Iron in Real Off-Highway Axles: Effects of Casting Skin and Strain Ratio
Authors: Scacco Federico , Campagnolo Alberto , Franceschi Mattia , Meneghetti Giovanni
Published: March 2022

Nodular cast irons are widely adopted in off-highway vehicles, since they allow to obtain components with complex shapes as well as good mechanical properties. However, the fatigue strength of such components is a major issue, which is typically addressed by adopting the strain-life approach, since it considers the local strains at stress concentrators where fatigue cracks are prone to initiate. In the fatigue design of off-highway components, the detrimental effect of casting skin, due to both surface and subsurface features, must be accounted for in all cases where machining is not needed. Moreover, the local strain ratio at stress raisers can be different from the nominal one due to forward plasticity. In this paper, static tensile as well as strain-controlled fatigue tests have been executed on specimens taken from real EN-GJS-450-10 off-highway axles. Static tensile tests have been performed on specimens with machined surfaces, while fatigue tests at a strain ratio equal to −1 have been carried out on specimens with surfaces in both machined and as-cast conditions. In addition, machined specimens have been tested at strain ratios equal to 0.1 and 0.5 to investigate the material sensitivity to the mean strain. Finally, the high-cycle downgrading effect of the casting skin has been evaluated, and experimental data of machined specimens tested at different strain ratios have been summarized by using the Smith–Watson–Topper expression.
The Use of Machine-Learning Techniques in Material Constitutive Modelling for Metal Forming Processes
Authors: Lourenço Rúben , Andrade-Campos António , Georgieva Pétia
Published: March 2022

Accurate numerical simulations require constitutive models capable of providing precise material data. Several calibration methodologies have been developed to improve the accuracy of constitutive models. Nevertheless, a model’s performance is always constrained by its mathematical formulation. Machine learning (ML) techniques, such as artificial neural networks (ANNs), have the potential to overcome these limitations. Nevertheless, the use of ML for material constitutive modelling is very recent and not fully explored. Difficulties related to data requirements and training are still open problems. This work explores and discusses the use of ML techniques regarding the accuracy of material constitutive models in metal plasticity, particularly contributing (i) a parameter identification inverse methodology, (ii) a constitutive model corrector, (iii) a data-driven constitutive model using empirical known concepts and (iv) a general implicit constitutive model using a data-driven learning approach. These approaches are discussed, and examples are given in the framework of non-linear elastoplasticity. To conveniently train these ML approaches, a large amount of data concerning material behaviour must be used. Therefore, non-homogeneous strain field and complex strain path tests measured with digital image correlation (DIC) techniques must be used for that purpose.
Correction Method of Three-Axis Magnetic Sensor Based on DA–LM
Authors: Yang Li , Li Caihong , Zhang Song , Xu Chaoqun , Chen Hun , Xiao Shuting , Tang Xiaoyu , Li Yongxin
Published: March 2022

The fluxgate magnetometer has the advantages of having a small volume and low power consumption and being light weight and is commonly used to detect weak magnetic targets, including ferrous metals, unexploded bombs (UXOs), and underground corrosion pipelines. However, the detection accuracy of the fluxgate magnetometer is affected by its own error. To obtain more accurate detection data, the sensor must be error-corrected before application. Previous researchers easily fell into the local minimum when solving error parameters. In this paper, the error correction method was proposed to tackle the problem, which combines the Dragonfly algorithm (DA) and the Levenberg–Marquardt (LM) algorithm, thereby solving the problem of the LM algorithm and improving the accuracy of solving error parameters. Firstly, we analyzed the error sources of the three-axis magnetic sensor and established the error model. Then, the error parameters were solved by using the LM algorithm and DA–LM algorithm, respectively. In addition, by comparing the results of the two methods, we found that the error parameters solved by using the DA–LM algorithm were more accurate. Finally, the magnetic measurement data were corrected. The simulation results show that the DA–LM algorithm can accurately solve the error parameters of the triaxial magnetic sensor, proving the effectiveness of the proposed algorithm. The experimental results show that the difference between the corrected and the ideal total value was decreased from 300 nT to 5 nT, which further verified the effectiveness of the DA–LM algorithm.
A Review on Material Extrusion Additive Manufacturing of Metal and How It Compares with Metal Injection Moulding
Authors: Suwanpreecha Chanun , Manonukul Anchalee
Published: March 2022

Material extrusion additive manufacturing of metal (metal MEX), which is one of the 3D printing processes, has gained more interests because of its simplicity and economics. Metal MEX process is similar to the conventional metal injection moulding (MIM) process, consisting of feedstock preparation of metal powder and polymer binders, layer-by-layer 3D printing (metal MEX) or injection (MIM) to create green parts, debinding to remove the binders and sintering to create the consolidated metallic parts. Due to the recent rapid development of metal MEX, it is important to review current research work on this topic to further understand the critical process parameters and the related physical and mechanical properties of metal MEX parts relevant to further studies and real applications. In this review, the available literature is systematically summarised and concluded in terms of feedstock, printing, debinding and sintering. The processing-related physical and mechanical properties, i.e., solid loading vs. dimensional shrinkage maps, sintering temperature vs. relative sintered density maps, stress vs. elongation maps for the three main alloys (316L stainless steel, 17-4PH stainless steel and Ti-6Al-4V), are also discussed and compared with well-established MIM properties and MIM international standards to assess the current stage of metal MEX development.
Microbiome, Mycobiome and Related Metabolites Alterations in Patients with Metabolic Syndrome—A Pilot Study
Authors: Gradisteanu Pircalabioru Gratiela , Ilie Iuliana , Oprea Luciana , Picu Ariana , Petcu Laura Madalina , Burlibasa Liliana , Chifiriuc Mariana-Carmen , Musat Madalina
Published: March 2022

Metabolic syndrome (MetSyn) has a rapidly growing worldwide prevalence, affecting over 1 billion people. MetSyn is clustering many pathological conditions, which, untreated, could increase the risk and often lead to more severe metabolic defects such as type 2 diabetes and non-alcoholic fatty liver disease. Many data demonstrate the complex role of gut microbiota in the host metabolism, and hence, deciphering the microbiome patterns linked to MetSyn could enable us for novel diagnosis and monitoring markers and for better disease management. Moreover, interventions designed to alter patient microbiome composition may help prevent or decrease morbidity linked with MetSyn. However, the microbiome composition is largely different across geographically distinct populations. Our study investigated the microbiota and mycobiome patterns in Romanian metabolic syndrome patients. We also correlated the identified microbiome–mycobiome patterns with levels of metabolites important for host health such as short chain fatty acids, organic acids, and taurine. We found that MetSyn patients are harboring a microbiome enriched in Enterobacteriaceae, Turicibacter sp., Clostridium coccoides, and Clostridium leptum, while beneficial taxa such as Butyricicoccus sp., Akkermansia muciniphila, and Faecalibacterium prausnitzii were decreased. These microbiome changes were correlated with lower butyrate levels and increased succinate. In terms of mycobiome signatures, MetSyn was associated with a high abundance of Saccharomyces and Aspergillus species. Our data are the first reported on a Romanian population and confirming that the pathogenesis of MetSyn is closely related to gut microbiome and homeostasis.
Impact of 3D Printing Technique and TPE Material on the Endurance of Pneumatic Linear Peristaltic Actuators
Authors: Fateri Miranda , Carneiro João Falcão , Schuler Constantin , Pinto João Bravo , Gomes de Almeida Fernando , Grabmeier Udo , Walcher Tobias , Salinas Michael
Published: March 2022

In this paper, additive manufacturing was used in order to produce hose prototypes for peristaltic linear pneumatic actuators. In order to optimise the endurance of the actuator, we 3D printed different thermoplastic polyurethane elastomers with different shore hardness levels using ARBURG Plastic Freeforming technology. Furthermore, effects of the hose geometries on the lifetime of the actuator were investigated. Experimental evidence showed that the lifetime of the actuator was dependent on the combination of the hose design and on the material used to manufacture the hose. Moreover, experimental tests showed that the use of the Aurburg-Freeformer 3D printing technology led to a much higher hose endurance than the one reported by using the fused layer manufacturing technique.
Design, Dynamics, and Optimization of a 3-DoF Nonlinear Micro-Gyroscope by Considering the Influence of the Coriolis Force
Authors: Wang Sai , Lu Linping , Zhang Kunpeng , Hao Shuying , Zhang Qichang , Feng Jingjing
Published: March 2022

In this paper, we use the nonlinear hardening stiffness of drive mode deal with the contradiction between gain and bandwidth of the linear micro-gyroscope, to improve the bandwidth and gain in sense direction. Firstly, in order to adjust the distance between two resonant peaks, we changed an incomplete two-degree-of-freedom(2-DoF) sense mode system of the micro-gyroscope into a complete 2-DoF system. Afterward, according to the given nonlinear coefficient of stiffness of drive mode, the structure size of driving micro-beams was designed to obtain a nonlinear micro-gyroscope with controllable stiffness. Finally, we investigated the effects of peaks spacing, damping, and driving nonlinearity on gain and bandwidth, and the nonlinear micro-gyroscope was optimized by orthogonal experiment method and response surface method. The results reveal that the peaks spacing has a great influence on the gain and bandwidth of both linear and nonlinear micro-gyroscopes. The larger the peaks spacing, the lower the gain, but higher gain can be achieved when the resonant frequency of the drive mode is close to the lower-order resonant frequency of the sense mode. Driving nonlinearity leads to the response peak of the Coriolis force to have a hardening characteristic, thus forming a wide platform in the sense direction. Hardening of the response peak of the Coriolis force allows the micro-gyroscope to obtain a higher gain while the bandwidth of the sense mode is also greatly improved. In addition, parameter optimization can make the gain and bandwidth of the micro-gyroscope optimal. When the peaks spacing is small and the nonlinear stiffness coefficient is about 1012.2, under the premise that the gain is basically constant, the bandwidth of the sense mode increases about 1.76 times compared with the linear gyroscope. Damping can suppress the influence of nonlinearity in a micro-gyroscope system. Within a certain range, the frequency response of the nonlinear micro-gyroscope tends to be a linear system with the increase in damping, resulting in narrower bandwidth and lower gain.
Multimodal Multidirectional Piezoelectric Vibration Energy Harvester by U-Shaped Structure with Cross-Connected Beams
Authors: Qin Hongbo , Mo Shuting , Jiang Xin , Shang Siyao , Wang Peng , Liu Yan
Published: March 2022

This paper proposes a multidirectional piezoelectric vibration energy harvester based on an improved U-shaped structure with cross-connected beams. Benefitting from the bi-directional capacity of U-shaped beam and additional bending mode induced by cross-connected configuration, the proposed structure can well capture the vibrations in 3D space at the frequencies lower than 15 Hz. These features are further validated by finite element analyses and theorical formulas. The prototype is fabricated and the experiments under different conditions are carried out. The results show that the proposed harvester can generate favorable voltage and power under multidirectional vibrations at a low operating frequency. Practical applications of charging capacitors and powering a wireless sensor node demonstrate the feasibility of this energy harvester in supplying power for engineering devices.
Distortion Calculation Method Based on Image Processing for Automobile Lateral Mirrors
Authors: Paredes-Orta Carlos , Valentin-Coronado Luis M. , Díaz-Ponce Arturo , Rodríguez-Reséndiz Juvenal , Mendiola-Santibañez Jorge Domingo
Published: March 2022

The automobile lateral-view mirrors are the most important visual support for driver safety; therefore, it is important they have robust quality control. Typically, the distortion of a lateral-view mirror is measured using the JIS-D-5705 standard; however, this methodology requires an expert person to perform the measurements and calculations manually, which can induce measurement errors. In this work, a semi-automatic distortion calculation method based on image processing is presented. Distortion calculations of five commercial mirrors from different manufacturers were performed, and a comparative study was carried out between the JIS-D-5705 standard and the proposed method. Experimental results performed according to the JIS-D-5705 standard showed that all mirrors have a distortion lower than 5%, indicating that all meet the standard. On the other hand, the proposed method was able to detect that one of the mirrors presented an important distortion, which was not detected by the methodology proposed in the standard; therefore, that mirror should not meet the standard. Then, it was possible to conclude that the proposed distortion calculation method, based on image processing, has higher robustness and precision than the standard. In addition, an appropriate and effective behavior against changes in scale, resolution, and, unlike the standard, against changes in image rotation was also shown.
Bioengineering Outlook on Cultivated Meat Production
Authors: Pajčin Ivana , Knežić Teodora , Savic Azoulay Ivana , Vlajkov Vanja , Djisalov Mila , Janjušević Ljiljana , Grahovac Jovana , Gadjanski Ivana
Published: March 2022

Cultured meat (also referred to as cultivated meat or cell-based meat)—CM—is fabricated through the process of cellular agriculture (CA), which entails application of bioengineering, i.e., tissue engineering (TE) principles to the production of food. The main TE principles include usage of cells, grown in a controlled environment provided by bioreactors and cultivation media supplemented with growth factors and other needed nutrients and signaling molecules, and seeded onto the immobilization elements—microcarriers and scaffolds that provide the adhesion surfaces necessary for anchor-dependent cells and offer 3D organization for multiple cell types. Theoretically, many solutions from regenerative medicine and biomedical engineering can be applied in CM-TE, i.e., CA. However, in practice, there are a number of specificities regarding fabrication of a CM product that needs to fulfill not only the majority of functional criteria of muscle and fat TE, but also has to possess the sensory and nutritional qualities of a traditional food component, i.e., the meat it aims to replace. This is the reason that bioengineering aimed at CM production needs to be regarded as a specific scientific discipline of a multidisciplinary nature, integrating principles from biomedical engineering as well as from food manufacturing, design and development, i.e., food engineering. An important requirement is also the need to use as little as possible of animal-derived components in the whole CM bioprocess. In this review, we aim to present the current knowledge on different bioengineering aspects, pertinent to different current scientific disciplines but all relevant for CM engineering, relevant for muscle TE, including different cell sources, bioreactor types, media requirements, bioprocess monitoring and kinetics and their modifications for use in CA, all in view of their potential for efficient CM bioprocess scale-up. We believe such a review will offer a good overview of different bioengineering strategies for CM production and will be useful to a range of interested stakeholders, from students just entering the CA field to experienced researchers looking for the latest innovations in the field.
High Occurrence of Zoonotic Subtypes of Cryptosporidiumparvum in Cypriot Dairy Farms
Authors: Hoque Sumaiya , Mavrides Daphne E. , Pinto Pedro , Costas Silvia , Begum Nisa , Azevedo-Ribeiro Claudia , Liapi Maria , Kváč Martin , Malas Stavros , Gentekaki Eleni , Tsaousis Anastasios D.
Published: March 2022

Cryptosporidium parvum is one of the major causes of neonatal calf diarrhoea resulting in reduced farm productivity and compromised animal welfare worldwide. Livestock act as a major reservoir of this parasite, which can be transmitted to humans directly and/or indirectly, posing a public health risk. Research reports on the prevalence of Cryptosporidium in ruminants from east Mediterranean countries, including Cyprus, are limited. This study is the first to explore the occurrence of Cryptosporidium spp. in cattle up to 24 months old on the island of Cyprus. A total of 242 faecal samples were collected from 10 dairy cattle farms in Cyprus, all of which were screened for Cryptosporidium spp. using nested-PCR amplification targeting the small subunit of the ribosomal RNA (18S rRNA) gene. The 60 kDa glycoprotein (gp60) gene was also sequenced for the samples identified as Cryptosporidium parvum-positive to determine the subtypes present. The occurrence of Cryptosporidium was 43.8% (106/242) with at least one positive isolate in each farm sampled. Cryptosporidium bovis, Cryptosporidium ryanae and C. parvum were the only species identified, while the prevalence per farm ranged from 20–64%. Amongst these, the latter was the predominant species, representing 51.8% of all positive samples, followed by C. bovis (21.7%) and C. ryanae (31.1%). Five C. parvum subtypes were identified, four of which are zoonotic—IIaA14G1R1, IIaA15G1R1, IIaA15G2R1 and IIaA18G2R1. IIaA14G1R1 was the most abundant, representing 48.2% of all C. parvum positive samples, and was also the most widespread. This is the first report of zoonotic subtypes of C. parvum circulating in Cyprus. These results highlight the need for further research into the parasite focusing on its diversity, prevalence, host range and transmission dynamics on the island.
Anti-Tumor Effects of Heat-Killed L. reuteri MG5346 and L. casei MG4584 against Human Colorectal Carcinoma through Caspase-9-Dependent Apoptosis in Xenograft Model
Authors: Kim Suk-Jin , Kang Chang-Ho , Kim Gun-Hee , Cho Hyosun
Published: March 2022

In this study, we examined the anti-tumor effects of heat-killed Bifidobacterium and Lactobacillus strains on human colorectal carcinoma RKO cells in in vitro and in vivo xenograft models. First, the cytotoxic and apoptotic effects of 11 different strains were examined using an MTT assay and flow cytometry, respectively. Then, xenograft BALB/c nude mice were implanted with RKO cells and orally administered with single or mixed heat-killed bacterial strains to examine their inhibitory effects on tumor growth. Additionally, the levels of cleaved caspase-9, -3, and -7 and PARP in tumor tissues were analyzed using Western blotting or immunohistochemistry staining. The results showed that RKO cells were highly susceptible to heat-killed B. bifidum MG731 and L. reuteri MG5346 and that L. casei MG4584 induced apoptosis to a greater extent than other strains. The oral administration of individual MG731, MG5346, or MG4584 significantly delayed tumor growth, and mixtures of MG5346 and MG4584 or MG731, MG5346, and MG4584 synergistically inhibited the tumor growth in the xenograft model. The expression of cleaved caspase-3, -7, and -9 and PARP in the tumor tissues was increased in Western blotting, and the expression of cleaved caspase-3 and PARP in immunohistochemistry staining was also increased. Therefore, we suggest that the use of the combination of MG5346 and MG4584 as parabiotics could effectively inhibit the growth of colorectal cancer.
Legionella pneumophila in Municipal Shower Systems in Stavanger, Norway; A Longitudinal Surveillance Study Using Whole Genome Sequencing in Risk Management
Authors: Krøvel Anne Vatland , Bernhoff Eva , Austerheim Elin , Soma Markus André , Romstad Monica Regine , Löhr Iren Høyland
Published: March 2022

Following an incidence of Legionnaires disease (LD) in 2007, where a municipal shower system was the likely source of infection, Stavanger municipality initiated a surveillance program for Legionella as part of establishing internal risk evaluation and prevention routines. More than 250 shower systems were examined for cultivatable Legionella pneumophila. The prevalence and diversity of serogroups (sg) and sequence types (STs) of L. pneumophila were mapped using available typing techniques over a period of more than 10 years (2010–2021). The surveillance showed an overall reduction in the L. pneumophila colonisation rate in municipal systems from 11 to 4.5% following prevention measures during the period, with the highest colonisation rate in complex systems (e.g., larger nursing homes and sports complexes). Further, an approximately even distribution between sg1 and 2–14 was seen. Whole genome sequencing (WGS) revealed that only a limited number of STs were detected, and they were consistent at specific locations over time. This study showed that environmental surveillance data in combination with available typing techniques and WGS can give the municipality a better tool for risk management and an overview of ST distributions that can be a valuable asset in future source investigations.
Continuous Culture of Auxenochlorella protothecoides on Biodiesel Derived Glycerol under Mixotrophic and Heterotrophic Conditions: Growth Parameters and Biochemical Composition
Authors: Korozi Evagelina , Tsagou Vasiliki , Kefalogianni Io , Markou Giorgos , Antonopoulos Dimitris , Chakalis Lambis , Kotzamanis Yannis , Chatzipavlidis Iordanis
Published: March 2022

As crude glycerol comprises a potential substrate for microalga fermentation and value added products’ biosynthesis, Auxenochlorella protothecoides was grown on it under heterotrophic and mixotrophic conditions and its growth kinetics were evaluated in a continuous system under steady state conditions. Increasing initial glycerol concentration (from 30 to 50 g/L) in the heterotrophic culture led to reduced biomass yield (Yx/S) and productivity (Px), but favored lipid accumulation. Under heterotrophic conditions, the microalga was found to grow better (biomass up to 7.888 g/L) and faster (higher growth rates), the system functioned more effectively (higher Px) and crude glycerol was exploited more efficiently. Heterotrophy also favored proteins synthesis (up to 53%), lipids (up to 9.8%), and carbohydrates (up to 44.6%) accumulation. However, different trophic modes had no significant impact on the consistency of proteins and lipids. Oleic acid was the most abundant fatty acid detected (55–61.2% of the total lipids). The algal biomass contained many essential and non-essential amino acids, especially arginine, glutamic acid, lysine, aspartic acid, leucine, and alanine. In all the experimental trials, the protein contents in the microalgal biomass increased with the increasing dilution rate (D), with a concomitant decrease in the lipids and carbohydrates fractions.
One-Step Synthesis of Iron and Titanium-Based Compounds Using Black Mineral Sands and Oxalic Acid under Subcritical Water Conditions
Authors: Valdivieso-Ramírez Carla S. , Pontón Patricia I. , Dosen Anja , Marinkovic Bojan A. , Guerrero Victor H.
Published: March 2022

Black mineral sands are widely used to obtain titanium dioxide, titanium, and, more recently, a variety of iron–titanium oxide nanostructures. Highly corrosive mineral acids or alkalis are commonly employed for this purpose. Hence, it is desirable to find eco-friendly ways to process these minerals, deriving high-added value materials. In this study, an Ecuadorian mineral sand precursor (0.6FeTiO3∙0.4Fe2O3 solid solution) was treated with oxalic acid aqueous solutions under subcritical water conditions. The synthesis was conducted in a batch reactor operating at 155 °C, 50 bar, and 700 rpm for 12 h, varying the oxalic acid concentration (0.1, 0.5 to 1.0 M). The as-obtained compounds were physically separated, dried, and analyzed by X-ray powder diffraction, scanning electron microscopy, and Raman spectroscopy. The characterization showed that the precursor was completely converted into two main products, ferrous oxalate, and titanium dioxide polymorphs. Rutile was always found in the as-synthesized products, while anatase only crystallized with high oxalic acid concentrations (0.5 and 1.0 M). These results open the possibility to develop more sustainable routes to synthesize iron and titanium-based materials with promising applications.