EFFICIENCY OF INDEZ DISINFECTANT APPLICATION IN PRODUCTION CONDITIONS
Authors: Kotsyumbas I.Ya. , Brezvyn O. M. , Rudyk H. V. , Ivashkiv Yu. A. 
Published: February 2021

The article presents the results of a study to research the effectiveness and safety of disinfectant "Indez" as a sanitizing substance for the prevention of bacterial infections of poultry, as well as its effect on the respiratory system. In the course of the experiment, broiler chickens from 2 poultry houses were used, where each of them had 10,000 heads of Cobb crosses, which were kept by the floor method. Before and after the use of disinfectant "Indez" measurements of total bacterial contamination were carried out, including sanitary-indicative (coliform bacteria) microflora of poultry, and also studied the effect of disinfectant on the morphological and biochemical parameters of poultry blood. Blood for morphological and biochemical studies was taken in the morning before feeding, by puncture of the axillary vein on the 5th, 10th and 20th day of the experiment. Morphological parameters were determined in the blood of broiler chickens, and in the serum were determined: total protein content, protein fractions, bilirubin level, alkaline phosphatase (LF) activity, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The investigated means "Indez" was used to rehabilitate the poultry house by uniformly sprinkling the surface where the chickens were kept at the rate of: the first sprinkling 80 g / m2, and the following - once a week 50 g / m2. In the poultry house, where the chickens of the control group were, the premises were not disinfected. Analysis of the obtained data on bacterial air pollution in both experimental poultry houses shows that after the use of the drug "Indez" the level of total bacterial contamination decreased in the poultry house № 1 in 3.35 times, including sanitary-indicative microflora in 2.83 times . In the poultry house № 2 after treatment with the test agent, the level of total bacterial contamination was - 573.3 thousand microns. cells / m3 of air, which is 1.5 times less than before treatment. At the same time, the level of coliform bacteria decreased by 1.68 times. The influence of disinfectant on the dynamics of hematological and biochemical parameters of broiler chickens is analyzed. In the study of morphological parameters of the blood of broiler chickens under the conditions of application of disinfectant "Indez" on the 20th day of the experiment in both groups of birds their hematological parameters did not differ statistically and fluctuated within the parameters of physiological norm:

hemoglobin concentration (95.6 ± 0.6 and 95.4 ± 2.04 g / l, respectively), the number of erythrocytes (3.6 ± 0.24 and 3.5 ± 0.16 T / l), leukocytes (23.8 ± 0.37 and 24.2 ± 0.58 G/l). When studying the biochemical parameters of the serum of broiler chickens, it was found that the activity of the enzymes AST, ALT and alkaline phosphatase in the experimental and control groups of chickens during the experiment remained at the same level - from 233.75 ± 3.36 to 238.67 ± 1.28 U/cm3, from 17.4 ± 1.21 to 20.28 ± 1.11 U/l and from 64.2 ± 1.88 to 65.20 ± 2.18 U/l, respectively. It should be noted that the serum of control and experimental birds after remediation "Indez" during the experiment, the content of total protein and albumin, as well as the level of α-, β- and γ-globulins were at the same level with slight fluctuations within the physiological norm: 58.02±1.07– 60.74±1.17 g/l and 33.2 ± 0.65–34.38 ± 1.1%, as well as 16.44 ± 0.68–17.39 ± 0.87%, 13.3 ± 0.91–14.92 ± 0.77% and 33.7 ± 1.03–36.9 ± 0.98%, respectively. The index of total bilirubin in the serum of the control and experimental groups of chickens was also within the parameters of the physiological norm both before and after disinfection of the poultry house in the presence of poultry: from 1.87 ± 0.28 to 2.13 ± 0.22 μmol / l . The displayed results indicate that the use of disinfectant "Indez" for disinfection in the presence of birds did not cause visible clinical changes in the body. The safety of the use of the drug "Indez" in poultry houses in the presence of poultry is proved by the results of hematological and biochemical studies, as well as confirmed by the results of pathological autopsy.