bluuebunny/crossref_metadata_2025 huggingface dataset:
Preventing a global transition to thermoacoustic instability by targeting local dynamics
Authors: George Nitin Babu , Raghunathan Manikandan , Unni Vishnu R , Sujith R. I. , Kurths Jürgen , Surovyatkina Elena
Published: June 2022

The burning of fossil fuels to generate power produces harmful emissions. Lowering such emissions in gas turbine engines is possible by operating them at fuel-lean conditions. However, such strategies often fail because, under fuel-lean conditions, the combustors are prone to catastrophic high-amplitude oscillations known as thermoacoustic instability. We reveal that, as an operating parameter is varied in time, the transition to thermoacoustic instability is initiated at specific spatial regions before it is observed in larger regions of the combustor. We use two indicators to discover such inceptive regions: the growth of variance of fluctuations in spatially resolved heat release rate and its spatiotemporal evolution. In this study, we report experimental evidence of suppression of the global transition to thermoacoustic instability through targeted modification of local dynamics at the inceptive regions. We strategically arrange slots on the flame anchor, which, in turn, reduce the local heat release rate fluctuations at the inceptive regions and thus suppress the global transition to thermoacoustic instability. Our results open new perspectives for combustors that are more environmental-friendly.
Empirical analyses and simulations showed that different machine and statistical learning methods had differing performance for predicting blood pressure
Authors: Austin Peter C. , Harrell Frank E. , Lee Douglas S. , Steyerberg Ewout W.
Published: June 2022

Machine learning is increasingly being used to predict clinical outcomes. Most comparisons of different methods have been based on empirical analyses in specific datasets. We used Monte Carlo simulations to determine when machine learning methods perform better than statistical learning methods in a specific setting. We evaluated six learning methods: stochastic gradient boosting machines using trees as the base learners, random forests, artificial neural networks, the lasso, ridge regression, and linear regression estimated using ordinary least squares (OLS). Our simulations were informed by empirical analyses in patients with acute myocardial infarction (AMI) and congestive heart failure (CHF) and used six data-generating processes, each based on one of the six learning methods, to simulate continuous outcomes in the derivation and validation samples. The outcome was systolic blood pressure at hospital discharge, a continuous outcome. We applied the six learning methods in each of the simulated derivation samples and evaluated performance in the simulated validation samples. The primary observation was that neural networks tended to result in estimates with worse predictive accuracy than the other five methods in both disease samples and across all six data-generating processes. Boosted trees and OLS regression tended to perform well across a range of scenarios.
Low expression of IGFBP4 and TAGLN accelerate the poor overall survival of osteosarcoma
Authors: Xi Yue , Liu Jianlin , Shen Gufeng
Published: June 2022

Osteosarcoma is a common malignant bone tumor characterized by the production of osteoid stroma by the tumor. However, effect of IGFBP4 and TAGLN on the survival of osteosarcoma is unclear. The GEO database was used to identify the differentially expressed genes (DEGs) between control samples and osteosarcoma. Genes for biological process (BP), cellular composition (CC), and molecular function (MF) were examined using DAVID, Metascape, and GSEA. GSE14359 and GSE36001 were downloaded in the GEO database. GEO2R was used to find DEGs between control samples and osteosarcoma. The cytoHubb also found the hub genes of IGFBP4 and TAGLN. The Kaplan–Meier method was used to analyze overall survival. A total of 134 patients with osteosarcoma were enrolled in this study. The RNA levels of IGFBP4 and TAGLN were evaluated by RT-qPCR. The correlation between IGFBP4 and TAGLN expression and their associations with clinical indicators were analyzed using Spearman's rho test and Pearson's Chi-squared test. Univariate and multivariate Cox regression analyses were used to determine the potential prognostic factors. And the animal model was used to verify the role of hub genes on the osteosarcoma by the RT-qPCR and immunofluorescence. Support Vector Machine (SVM) was performed to construct the correlation among the expression of IGFBP4, TAGLN, and osteosarcoma. Through bioinformatics, IGFBP4 and TAGLN were identified as the hub genes of osteosarcoma. And osteosarcoma patients with high expression levels of IGFBP4 (HR = 0.56, P = 0.013) and TAGLN (HR = 0.52, P = 0.012) had better overall survival times than those with low expression levels. The results showed that pathologic grade (P = 0.017), tumor metastasis (P < 0.001), and enneking stage (P < 0.001) were significantly correlated with IGFBP4. Also, pathologic grade (P = 0.002), tumor metastasis (P < 0.001), and enneking stage (P < 0.001) were significantly related to the TAGLN. Spearman’s correlation coefficient displayed that IGFBP4 were significantly correlated with the tumor metastasis (ρ = − 0.843, P < 0.001), enneking stage (ρ = − 0.500, P < 0.001), and TAGLN (ρ = 0.821, P < 0.001). IGFBP4 (HR = 0.252, 95% CI 0.122–0.517, P < 0.001) and TAGLN (HR = 0.155, 95% CI 0.089–0.269, P < 0.001) were significantly associated with overall survival. Based on the qPCR and immunofluorescence, IGFBP4 and TAGLN were down-regulated in the osteosarcoma tissue than the control group. And the SVM presented that there exists strong relationship among the expression of IGFBP4, TAGLN, and osteosarcoma. IGFBP4 and TAGLN may be attractive molecular targets for osteosarcoma, opening a new avenue for research into the disease.
Coevolution of diagenetic fronts and fluid-fracture pathways
Authors: Koeshidayatullah Ardiansyah , Al-Sinawi Nawwar , Swart Peter K. , Boyce Adrian , Redfern Jonathan , Hollis Cathy
Published: June 2022

Diagenetic boundaries are paleo-reaction fronts, which have the potential to archive the termination of metasomatic processes in sedimentary rocks. They have not been extensively studied, perhaps because they appear simple in outcrop. Recent work has demonstrated the significance of paleo-reaction fronts to decipher multiphase recrystallization processes and provide high porosity zones. This paper provides a detailed documentation of reaction front evolution in a tectonically active salt basin and reveals a high level of complexity, associated with multiple fluid flow and tectonic events. Here, consistent patterns of increasing dolomite stoichiometry and ordering, along with a change from seawater-derived, fabric-retentive dolomite to fracture-controlled, fabric-destructive hydrothermal dolomite are observed vertically across the stratabound dolomite bodies. These patterns, coupled with a decrease in porosity, increase in ∆47temperature and δ18Owatervalues indicate multiphase recrystallization and stabilization by warm, Mg-rich fluids. The stratabound dolomite bodies apparently terminated at a fracture-bound contact, but the presence of dolomite fragments within the fracture corridor suggests that fracturing post-dated the first dolomitization event. The termination of dolomite formation is therefore interpreted to be associated with a decrease in the capacity of the magnesium-rich fluids to dolomitize the rock, as indicated by the presence of non-stoichiometric and poorly ordered dolomite at the reaction fronts. The fracture corridors are interpreted to exploit dolostone-limestone boundaries, forming prior to a later, higher temperature, hydrothermal dolomitization event, which coincided with the formation and growth of the anticline. Karstification subsequently exploited these fracture corridors, widening fractures and leading to localized collapse and brecciation. The results demonstrate that an apparently simple reaction front can have a complex history, governed by the inheritance of prior diagenetic events. These events modified rock properties in such a way that fluid flow was repeatedly focused along the original dolomite-limestone boundary, overprinting much of its original signature. These findings have implications to the prediction of structurally controlled diagenetic processes and the exploration of naturally fractured carbonate reservoirs for energy exploration globally.
HLA class II variants defined by next generation sequencing are associated with sarcoidosis in Korean patients
Authors: Sikorová Kateřina , Moon Su-Jin , Yoon Hee-Young , Strnad Adam , Song Jin Woo , Petrek Martin
Published: June 2022

Polymorphic genes with immune functions, namely those of the human leukocyte antigen (HLA) system, have been implicated in sarcoidosis pathogenesis. As HLA polymorphisms in sarcoidosis have not been yet investigated in the Korean population, we used next-generation sequencing (NGS), allowing detailed characterization of HLA alleles to investigate the role of HLA variation in Korean sarcoidosis patients. We enrolled 103 patients diagnosed by the ATS/ERS/WASOG guidelines at Asan Medical Centre, Seoul, Korea. Among those, genotyping of 7 HLA loci (HLA-A, -B, -C, -DQA1, -DQB1, -DRB1, -DPB1) was performed using Omixon Holotype™ kit and HLATwin software™. HLA allele frequencies were compared with frequency data on healthy Koreans from the allelic frequency databases, and 4-digit characteristics of HLA genotyping were used. Associations were assessed by two-tailed Fischer’s exact test with correction for multiple comparisons. Variants previously associated with sarcoidosis risk (HLA-C*03:04, HLA-DRB1*12:01, HLA-DRB1*14:54) and a known protective variant HLA-DPB1*04:01, were associated with sarcoidosis in Koreans. Further, we suggest new HLA variants associated with sarcoidosis risk (e.g., HLA-DQA1*05:08) and novel protective variants HLA-DQB1*03:02 and HLA-DQA1*01:02 in Koreans. This first study of HLA variation in Korean patients with sarcoidosis by precise genotyping methodology reports data that could serve future meta-analyses on HLA variation’s role in sarcoidosis.
Automated soccer head impact exposure tracking using video and deep learning
Authors: Rezaei Ahmad , Wu Lyndia C.
Published: June 2022

Head impacts are highly prevalent in sports and there is a pressing need to investigate the potential link between head impact exposure and brain injury risk. Wearable impact sensors and manual video analysis have been utilized to collect impact exposure data. However, wearable sensors suffer from high deployment cost and limited accuracy, while manual video analysis is a long and resource-intensive task. Here we develop and apply DeepImpact, a computer vision algorithm to automatically detect soccer headers using soccer game videos. Our data-driven pipeline uses two deep learning networks including an object detection algorithm and temporal shift module to extract visual and temporal features of video segments and classify the segments as header or nonheader events. The networks were trained and validated using a large-scale professional-level soccer video dataset, with labeled ground truth header events. The algorithm achieved 95.3% sensitivity and 96.0% precision in cross-validation, and 92.9% sensitivity and 21.1% precision in an independent test that included videos of five professional soccer games. Video segments identified as headers in the test data set correspond to 3.5 min of total film time, which can be reviewed through additional manual video verification to eliminate false positives. DeepImpact streamlines the process of manual video analysis and can help to collect large-scale soccer head impact exposure datasets for brain injury research. The fully video-based solution is a low-cost alternative for head impact exposure monitoring and may also be expanded to other sports in future work.
RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
Authors: Guénolé Aude , Velilla Fabien , Chartier Aymeric , Rich April , Carvunis Anne-Ruxandra , Sardet Claude , Simonelig Martine , Sobhian Bijan
Published: June 2022

Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization. Here, we show that the RING finger protein RNF219 co-purifies with the CCR4-NOT complex, the major mRNA deadenylase in eukaryotes, which mediates translational repression in both a deadenylase activity-dependent and -independent manner. Strikingly, RNF219 both inhibits the deadenylase activity of CCR4-NOT and enhances its capacity to repress translation of a target mRNA. We propose that the interaction of RNF219 with the CCR4-NOT complex directs the translational repressive activity of CCR4-NOT to a deadenylation-independent mechanism.
A simple cut and stretch assay to detect antimicrobial resistance genes on bacterial plasmids by single-molecule fluorescence microscopy
Authors: Goyal Gaurav , Ekedahl Elina , Nyblom My , Krog Jens , Fröbrant Erik , Brander Magnus , Sewunet Tsegaye , Tangkoskul Teerawit , Giske Christian G. , Sandegren Linus , Thamlikitkul Visanu , Ambjörnsson Tobias , Westerlund Fredrik
Published: June 2022

Antimicrobial resistance (AMR) is a fast-growing threat to global health. The genes conferring AMR to bacteria are often located on plasmids, circular extrachromosomal DNA molecules that can be transferred between bacterial strains and species. Therefore, effective methods to characterize bacterial plasmids and detect the presence of resistance genes can assist in managing AMR, for example, during outbreaks in hospitals. However, existing methods for plasmid analysis either provide limited information or are expensive and challenging to implement in low-resource settings. Herein, we present a simple assay based on CRISPR/Cas9 excision and DNA combing to detect antimicrobial resistance genes on bacterial plasmids. Cas9 recognizes the gene of interest and makes a double-stranded DNA cut, causing the circular plasmid to linearize. The change in plasmid configuration from circular to linear, and hence the presence of the AMR gene, is detected by stretching the plasmids on a glass surface and visualizing by fluorescence microscopy. This single-molecule imaging based assay is inexpensive, fast, and in addition to detecting the presence of AMR genes, it provides detailed information on the number and size of plasmids in the sample. We demonstrate the detection of several β-lactamase-encoding genes on plasmids isolated from clinical samples. Furthermore, we demonstrate that the assay can be performed using standard microbiology and clinical laboratory equipment, making it suitable for low-resource settings.
Premorbid cancer and motor reserve in patients with Parkinson’s disease
Authors: Oh Yoon-Sang , Yoo Sang-Won , Lyoo Chul Hyoung , Lee Kwang-Soo , Kim Joong-Seok
Published: June 2022

Decreased cancer risk has been reported in patients with Parkinson’s disease (PD), and cancer prior to PD can have a protective effect on PD risk. We investigated cancer history prior to PD diagnosis to determine if such history can enhance motor reserve in PD by assessing the association between motor deficits and striatal subregional dopamine depletion. A total of 428 newly diagnosed, drug-naïve PD patients was included in the study. PD patients were categorized into three groups of no prior neoplasia, premorbid precancerous condition, and premorbid malignant cancer before PD diagnosis. Parkinsonian motor status was assessed using the Unified Parkinson’s Disease Rating Scale (UPDRS) motor score and modified Hoehn and Yahr stage score. All patients underwent positron emission tomography (PET) with 18F-N-(3-fluoropropyl)-2beta-carbon ethoxy-3beta-(4-iodophenyl) nortropane (18F-FP-CIT), and the regional standardized uptake value ratios (SUVRs) were analyzed with a volume-of-interest template among the groups. The UPDRS motor score negatively correlated with SUVRs in the posterior putamen for all patient groups. Groups with neoplasia, especially those with premorbid cancer, showed lower motor scores despite similar levels of dopamine depletion in the posterior putamen relative to those without neoplasia. These results suggest that premorbid cancer acts as a surrogate for motor reserve in patients with PD and provide imaging evidence that history of cancer has a protective effect on PD.
Prognostic implication of IgG4 and IgG1-positive cell infiltration in the lung in patients with idiopathic interstitial pneumonia
Authors: Komatsu Masamichi , Yamamoto Hiroshi , Uehara Takeshi , Kobayashi Yukihiro , Hozumi Hironao , Fujisawa Tomoyuki , Miyamoto Atsushi , Kishaba Tomoo , Kunishima Fumihito , Okamoto Masaki , Kitamura Hideya , Iwasawa Tae , Matsushita Shoichiro , Terasaki Yasuhiro , Kunugi Shinobu , Ushiki Atsuhito , Yasuo Masanori , Suda Takafumi , Hanaoka Masayuki
Published: June 2022

Immunoglobulin (Ig) G4-positive cells are rarely observed in the lungs of patients with idiopathic interstitial pneumonias (IIPs). IgG1 may be more pathogenic than IgG4, with IgG4 having both pathogenic and protective roles in IgG4-related disease (IgG4-RD). However, the role of both IgG1 and IgG4 in IIPs remains unclear. We hypothesized that patients with IgG4-positive interstitial pneumonia manifest different clinical characteristics than patients with IgG4-RD. Herein, we identified the correlation of the degree of infiltration of IgG1- and IgG4-positive cells with IIP prognosis, using a Japanese nationwide cloud-based database. We included eighty-eight patients diagnosed with IIPs after multidisciplinary discussion, from April 2009 to March 2014. IgG4-positive cell infiltration was identified in 12/88 patients with IIPs and 8/41 patients with idiopathic pulmonary fibrosis (IPF). Additionally, 31/88 patients with IIPs and 19/41 patients with IPF were diagnosed as having IgG1-positive cell infiltration. IgG4-positive IIPs tended to have a better prognosis. Conversely, overall survival in cases with IgG1-positive IPF was significantly worse. IIPs were prevalent with IgG1- or IgG4-positive cell infiltration. IgG1-positive cell infiltration in IPF significantly correlated with a worse prognosis. Overall, evaluating the degree of IgG1-positive cell infiltration may be prognostically useful in cases of IPF.
Genetic structure of Sclerotinia sclerotiorum populations from sunflower and cabbage in West Azarbaijan province of Iran
Authors: Faraghati Masoumeh , Abrinbana Masoud , Ghosta Youbert
Published: June 2022

Sclerotinia sclerotiorum is one of the most destructive fungal pathogens infecting a wide array of plant species worldwide. Management of this pathogen relies on the coordinated use of fungicides and resistant host cultivars with other control measures, but the effectiveness of these methods requires knowledge of the genetic variability and structure of the fungal populations. To provide insight into the genetic diversity and structure of this pathogen in West Azarbaijan province of Iran, a total of 136 isolates were collected from symptomatic sunflower and cabbage plants within fields in three regions and analysed using inter-simple sequence repeat (ISSR) markers and intergenic spacer (IGS) region of the rRNA gene sequences. A total of 83 ISSR multilocus genotypes (MLGs) were identified, some of which were shared among at least two regional or host populations but in a low frequency. High genotypic diversity, low levels of clonal fraction, and random association of ISSR loci in a region indicated a low level of clonal reproduction, and possibly a high level of sexually recombining life cycle for the pathogen in the province. Marker analyses revealed that the pathogen was spatially homogeneous among fields, and thus similar control measures, such as the choice of resistant cultivars and fungicides, may effectively manage S. sclerotiorum within the region. Four IGS haplotypes (IGS1–IGS4) were detected within populations with IGS3 being the most prevalent haplotype. The low IGS haplotype diversity, the absence of spatial structure, and shared MLGs among populations may suggest a single introduction and subsequent dispersal of S. sclerotiorum within West Azarbaijan province.
Experimental and simulation study on heat transfer characteristics of aluminium alloy piston under transition conditions
Authors: Liu Yang , Lei Jilin , Wang Dongfang , Deng Xiwen , Wen Jun , Wen Zhigao
Published: June 2022

In order to explore the thermal load change of the diesel engine piston under transitional conditions, and the influence of the position of cooling gallery on the heat transfer characteristics of the piston. An off-road high-pressure common-rail diesel engine is chosen as the research object. The sequence coupling method is used to establish the fluid–solid coupling heat transfer simulation model of the piston-gallery under the transition conditions of cold start, urgent acceleration and rapid deceleration. The Pareto optimization algorithm is introduced to optimize the position of the cooling gallery to reduce the maximum temperature and maximum thermal stress of the piston. The results show that the maximum temperature of the piston can be reduced by reducing the distance between the cooling gallery and the throat area under the maximum torque condition, and that the maximum thermal stress of the piston can be reduced by reducing the distance between the cooling gallery and the throat area or by increasing the distance between the cooling gallery and the ring area. Compared with the original design, the maximum temperature of Design A decreases by 1.28 °C while the maximum thermal stress decreases by 2.07 MPa. The maximum temperature and maximum thermal stress of Design B decreases by 0.22 °C and 0.5 MPa, respectively. The maximum thermal stress of Design C decreases by 2.67 MPa when the maximum temperature increases by 1.15 °C. The maximum change in temperature of the three typical designs and the original design of the piston throat under cold start, urgent acceleration and rapid deceleration conditions reached 207.29 °C, 136.78 °C and 9.89 °C, and the maximum change of thermal stress reached 8.62 MPa, 20.43 MPa, 4.08 MPa, respectively. The maximum change in temperature of the piston first ring groove under cold start, urgent acceleration and rapid deceleration conditions reached 172.00 °C, 83.52 °C and 7.36 °C, and the maximum change in thermal stress reached 22.96 MPa, 43.10 MPa, 5.72 MPa, respectively. The conclusions obtained can provide boundary conditions for further study of the thermal load change law of the same type of pistons, and also provide a theoretical basis for diesel engine piston structure optimization and the performance improvement.
Potential anti-hepatocellular carcinoma properties and mechanisms of action of clerodane diterpenes isolated from Polyalthia longifolia seeds
Authors: Tatipamula Vinay Bharadwaj , Thonangi Chandi Vishala , Dakal Tikam Chand , Vedula Girija Sastry , Dhabhai Bhanupriya , Polimati Haritha , Akula Annapurna , Nguyen Ha Thi
Published: June 2022

Diterpenes are secondary metabolites that have attracted much attention due to their potential biological activities including anti-cancer potential. The aim of the current study is to assess the anticancer potential of the six known clerodane diterpenes (1–6) isolated fromPolyalthia longifoliaseeds and their underlying molecular mechanisms. These compounds were evaluated for their cytotoxicity in vitro by using MTT assays. The “two-phase model” with NDEA and PB ad libitum was used for induction of HCC and sorafenib was used as the standard drug. Prophylactic studies were carried out for compounds4/6at both low (5 mg/kg b.w) and high (10 mg/kg b.w) doses. Based on the MTT assay results, the two best compounds,4and6, were selected for in vivo studies. The results showed that treatment with compound4/6significantly restored the changes in biochemical parameters and liver morphology observed in (NDEA + PB)-induced HCC rats. Additionally, the docking studies showed that compound4/6interacted with several key proteins such as MDM2, TNF-α, FAK, thereby inhibiting these proteins and reversing the negative impacts of NDEA. In conclusion, our results suggested that compounds4and6are potential therapeutic agents for HCC, mostly due to their ability to control typical cancer pathways.
5 days of time-restricted feeding increases fat oxidation rate but not affect postprandial lipemia: a crossover trial
Authors: Chiu Chih-Hui , Chen Che-Hsiu , Wu Min-Huan , Lan Pei-Tzu , Hsieh Yu-Chen , Lin Zong-Yan , Chen Bo-Wei
Published: June 2022

Studies have revealed that time-restricted feeding affects the fat oxidation rate; however, its effects on the fat oxidation rate and hyperlipidemia following high-fat meals are unclear. This study investigated the effects of 5-day time-restricted feeding on the fat oxidation rate and postprandial lipemia following high fat meals. In this random crossover experimental study, eight healthy male adults were included each in the 5-day time-restricted feeding trial and the control trial. The meals of the time-restricted feeding trial were provided at 12:00, 16:00, and 20:00. The meals of the control trial were provided at 08:00, 14:00, and 20:00. The contents of the meals of both trials were the same, and the calories of the meals met the 24-h energy requirement of the participants. After 5 days of the intervention, the participants consumed high-fat meals on the sixth day, and their physiological changes were determined. The fasting fat oxidation rate (p < 0.001) and postprandial fat oxidation rate (p = 0.019) of the time-restricted feeding trial were significantly higher than those of the control trial. The 24-h energy consumption and postprandial triglyceride, blood glucose, insulin, glycerol, and free fatty acid concentrations of the two trials showed no significant differences (p > 0.05). The results revealed that 5 days of time-restricted feeding effectively increased the fasting and postprandial fat oxidation rate, but it did not affect postprandial lipemia.
Characterization of an aerosolized nanoparticle beam beyond the diffraction limit through strong field ionization
Authors: Davino Michael , Saule Tobias , Helming Nora G. , Powell J. A. , Trallero-Herrero Carlos
Published: June 2022

The study of nanomaterials is an active area of research for technological applications as well as fundamental science. A common method for studying properties of isolated nanoparticles is by an in-vacuum particle beam produced via an aerodynamic lens. Despite being common practice, characterization of such beams has proven difficult as light scattering detection techniques fail for particles with sizes beyond the diffraction limit. Here we present a new technique for characterizing such nanoparticle beams using strong field ionization. By focusing an ultrafast, mJ-level laser into the particle beam, a nanoparticle within the laser focus is ionized and easily detected by its ejected electrons. This method grants direct access to the nanoparticle density at the location of the focus, and by scanning the focus through the transverse and longitudinal profiles of the particle beam we attain the 3-dimensional particle density distribution for a cylindrically symmetric beam. Further, we show that strong field ionization is effective in detecting spherical nanoparticles as small as 10 nm in diameter. Additionally, this technique is an effective tool in optimizing the particle beam for specific applications. As an example we show that the particle beam density and width can be manipulated by restricting the gas flow into the aerodynamic lens.
Evaluating diagnostic accuracy and determining optimal diagnostic thresholds of different approaches to [68Ga]-DOTATATE PET/MRI analysis in patients with meningioma
Authors: Kim Sean H. , Roytman Michelle , Madera Gabriela , Magge Rajiv S. , Liechty Benjamin , Ramakrishna Rohan , Pannullo Susan C. , Schwartz Theodore H. , Karakatsanis Nicolas A. , Osborne Joseph R. , Lin Eaton , Knisely Jonathan P. S. , Ivanidze Jana
Published: June 2022

Multiple approaches with [68Ga]-DOTATATE, a somatostatin analog PET radiotracer, have demonstrated clinical utility in evaluation of meningioma but have not been compared directly. Our purpose was to compare diagnostic performance of different approaches to quantitative brain [68Ga]-DOTATATE PET/MRI analysis in patients with suspected meningioma recurrence and to establish the optimal diagnostic threshold for each method. Patients with suspected meningioma were imaged prospectively with [68Ga]-DOTATATE brain PET/MRI. Lesions were classified as meningiomas and post-treatment change (PTC), using follow-up pathology and MRI as reference standard. Lesions were reclassified using the following methods: absolute maximum SUV threshold (SUV), SUV ratio (SUVR) to superior sagittal sinus (SSS) (SUVRsss), SUVR to the pituitary gland (SUVRpit), and SUVR to the normal brain parenchyma (SUVRnorm). Diagnostic performance of the four methods was compared using contingency tables and McNemar’s test. Previously published pre-determined thresholds were assessed where applicable. The optimal thresholds for each method were identified using Youden’s J statistics. 166 meningiomas and 41 PTC lesions were identified across 62 patients. SUV, SUVRsss, SUVRpit, and SUVRnorm of meningioma were significantly higher than those of PTC (P < 0.0001). The optimal thresholds for SUV, SUVRsss, SUVRpit, and SUVRnorm were 4.7, 3.2, 0.3, and 62.6, respectively. At the optimal thresholds, SUV had the highest specificity (97.6%) and SUVRsss had the highest sensitivity (86.1%). An ROC analysis of SUV, SUVRsss, SUVRpit, and SUVRnorm revealed AUC of 0.932, 0.910, 0.915, and 0.800, respectively (P < 0.0001). Developing a diagnostic threshold is key to wider clinical translation of [68Ga]-DOTATATE PET/MRI in meningioma evaluation. We found that the SUVRsss method may have the most robust combination of sensitivity and specificity in the diagnosis of meningioma in the post-treatment setting, with the optimal threshold of 3.2. Future studies validating our findings in different patient populations are needed to continue optimizing the diagnostic performance of [68Ga]-DOTATATE PET/MRI in meningioma patients.

Trial registration: ClinicalTrials.gov Identifier: NCT04081701. Registered 9 September 2019. https://clinicaltrials.gov/ct2/show/NCT04081701.
Activation of the TGF-β1/Smads/α-SMA pathway is related to histological and functional changes in children with neurogenic bladder
Authors: Yang Xinghuan , Pu Qingsong , Wen Yibo , Zhao Yi , Wang Junkui , Xu Pengchao , Ma Yuan , Liu Erpeng , Lv Lei , Wen Jian Guo
Published: June 2022

This research is to investigate the expression of the TGF-β1/Smads/α-SMA pathway and its effect on bladder histology and function in children with neurogenic bladder (NB). The bladder specimens from 10 children with NB and 8 children with vesicoureteral junction obstruction were collected into the NB and control groups. The expression of TGF-β1, Smad2, Smad3, Smad4, Smad6, α-SMA, fibronectin, collagen I and collagen III in bladder tissues was detected. In addition, the histological characteristics of the bladder were evaluated. A preoperative urodynamic study was performed on all children with NB. We analysed the correlations among the expression of the marker protein a-SMA in myofibroblasts, effector cells of the pathway, and bladder function parameters. Compared with those in the control group, the expression of TGF-β1, Smad2, Smad3, Smad4, α-SMA, fibronectin, collagen I and collagen III was significantly increased in the NB group, while the expression of Smad6 was decreased (p < 0.01). HE and Masson staining in the NB group showed increased collagen levels and hypertrophy of smooth muscle cells. Children with NB had a low bladder volume ratio (BVR), low compliance (△C) and high maximum bladder pressure, low maximum flow rate, large postvoid residual volume, low bladder contraction index and low bladder voiding efficiency. The expression of α-SMA was negatively correlated with the BVR (r =  − 0.7066, P = 0.0223) and △C (r =  − 0.6516, P = 0.0412). We conclude that the TGF-β1/Smads/α-SMA pathway is activated in the bladder tissue of children with NB and may be involved in the processes causing histological and functional changes.
Reliable fluorescence technique to detect the antibiotic colistin, a possible environmental threat due to its overuse
Authors: Mandal Saurodeep , Dey Bhowmik Arpan , Mukhuty Alpana , Kundu Shampa , Truong Khai-Nghi , Rissanen Kari , Chattopadhyay Ansuman , Sahoo Prithidipa
Published: June 2022

Colistin, considered a drug of last resort as it is effective towards multidrug-resistant Gram-negative bacterial infections. Oral administration of colistin in the poultry industry is a common practice, not only to prevent and reduce bacterial infections, but also as a rapid-growth promoter. Long-term exposure to any antibiotic will eventually lead to the development of bacterial resistance towards all antibiotics through various mechanisms in the physiological system and environment. Chicken is the most consumed source of animal protein for humans throughout the world. In addition, the manure of poultry, containing traces of the used antibiotics, is being used in farming. Exposure to excess amounts of colistin causes a great concern not only to the humans but to the environment as a whole. In the present contribution, colistin has been detected in chicken hepatocyte cells through in vivo confocal microscopy. In addition, the amount of colistin in the chicken excrements has been estimated. A simple chemosensor NAF, a dye-based on napthaldehyde furfural, was developed for the detection of colistin, supplemented with experimental evidence and theoretical calculations.
Exploration of serum biomarkers in dogs with malignant melanoma receiving anti-PD-L1 therapy and potential of COX-2 inhibition for combination therapy
Authors: Maekawa Naoya , Konnai Satoru , Asano Yumie , Sajiki Yamato , Deguchi Tatsuya , Okagawa Tomohiro , Watari Kei , Takeuchi Hiroto , Takagi Satoshi , Hosoya Kenji , Kim Sangho , Ohta Hiroshi , Kato Yukinari , Suzuki Yasuhiko , Murata Shiro , Ohashi Kazuhiko
Published: June 2022

Immune checkpoint inhibitors (ICIs) such as anti-PD-L1 antibodies are widely used to treat human cancers, and growing evidence suggests that ICIs are promising treatments for canine malignancies. However, only some canine oral malignant melanoma (OMM) cases respond to ICIs. To explore biomarkers predictive of survival in dogs with pulmonary metastatic OMM receiving the anti-PD-L1 antibody c4G12 (n = 27), serum concentrations of prostaglandin E2 (PGE2), cytokines, chemokines, and growth factors were measured prior to treatment initiation. Among 12 factors tested, PGE2, interleukin (IL)-12p40, IL-8, monocyte chemotactic protein-1 (MCP-1), and stem cell factor (SCF) were higher in OMM dogs compared to healthy dogs (n = 8). Further, lower baseline serum PGE2, MCP-1, and vascular endothelial growth factor (VEGF)-A concentrations as well as higher IL-2, IL-12, and SCF concentrations predicted prolonged overall survival. These observations suggest that PGE2 confers resistance against anti-PD-L1 therapy through immunosuppression and thus is a candidate target for combination therapy. Indeed, PGE2 suppressed IL-2 and interferon (IFN)-γ production by stimulated canine peripheral blood mononuclear cells (PBMCs), while inhibition of PGE2 biosynthesis using the COX-2 inhibitor meloxicam in combination with c4G12 enhanced Th1 cytokine production by PBMCs. Thus, serum PGE2 may be predictive of c4G12 treatment response, and concomitant use of COX-2 inhibitors may enhance ICI antitumor efficacy.
Occurrence and seasonal disparity of emerging endocrine disrupting chemicals in a drinking water supply system and associated health risk
Authors: Kumawat Manoj , Sharma Poonam , Pal Namrata , James Meenu Mariya , Verma Vinod , Tiwari Rajnarayan R. , Shubham Swasti , Sarma Devojit Kumar , Kumar Manoj
Published: June 2022

Contamination of drinking water with endocrine-disrupting chemicals (EDCs) raises concerns over the security and long-term sustainability of clean water supplies as well as human exposure via daily water intake. In this study, the seasonal disparity and occurrence of six phthalates and bisphenol-A in the drinking water supply system and associated health-risk were examined. The detection frequencies of the ∑6PAEs ranged from 24 to 100% in the winter whereas; in summer it is below the detection limit up to 100%. DEHP was the most prevalent phthalate congener ranging from 1.14 to 8351.85 µg/L (winter) and 0.552 to 410.29 µg/L (summer) surpassing the permissible limit. However, BPA concentrations were found under the permissible limit. The results suggested that PAEs concentration displayed significant seasonal variations with the highest in winter and the lowest in summer. The exposure to PAEs and BPA from drinking water was assessed, indicating a possible health risk to humans with a hazard quotient (HQ) > 1 for DEHP only. The findings necessitate an immediate scrutiny of these EDCs in drinking water supply system and are critical for implementing effective technologies at the WTP scale to ensure the quality and safety of drinking water to ascertain human and environmental health.
Stress concentration-relocating interposer in electronic textile packaging using thermoplastic elastic polyurethane film with via holes for bearing textile stretch
Authors: Takamatsu Seiichi , Sato Suguru , Itoh Toshihiro
Published: June 2022

Electronic textile (e-textile) devices require mechanically reliable packaging that can bear up to 30% stretch induced by textile crimp stretch, because the boundary between the rigid electronic components and the soft fabric circuit in the e-textile is prone to rupture due to mismatch of their mechanical properties. Here, we describe a thin stress-concentration-relocating interposer that can sustain a textile stretch of up to 36%, which is greater than the 16% stretch of conventional packaging. The stress-concentration-relocating interposer consists of thin soft thermoplastic polyurethane film with soft via holes and is inserted between the electronic components and fabric circuit in order to move the area of stress concentration from the wiring area of the fabric circuit to the film. A finite element method (FEM) simulation showed that when the fabric is stretched by 30%, the boundary between the electrical components and the insulation layer is subjected to 90% strain and 2.5 MPa stress, whereas, at 30% strain, the boundary between the devices and the wiring is subjected to only 1.5 MPa stress, indicating that the concentration of stress in the wiring is reduced. Furthermore, it is shown that an optimal interposer structure that can bear a 30% stretch needs insulating polyurethane film in excess of 100 μm thick. Our thin soft interposer structure will enable LEDs and MEMS sensors to withstand stretching in several types of fabric.
Higher neutrophil–lymphocyte ratio is associated with depressive symptoms in Japanese general male population
Authors: Kinoshita Hirotaka , Takekawa Daiki , Kudo Takashi , Sawada Kaori , Mikami Tatsuya , Hirota Kazuyoshi
Published: June 2022

Relationships between the neutrophil–lymphocyte ratio (NLR) and/or the platelet-lymphocyte ratio (PLR) and neuroinflammatory diseases have been reported. Depression is also associated with neuroinflammation. Here, we determined the association between the NLR, PLR, and depressive symptoms. This cross-sectional study is a secondary analysis of the data of the Iwaki Health Promotion Project 2017. We analyzed the characteristics and laboratory data of 1,015 Japanese subjects (597 females, 408 males) including their NLR and PLR values. We assigned the subjects with a Center for Epidemiologic Studies Depression Scale (CES-D) score ≥ 16 to the depressive symptoms group. We performed a multivariate logistic regression analysis to determine whether the NLR and/or PLR were associated with depressive symptoms (CES-D ≥ 16). Two hundred subjects (19.7%; 122 [20.4%] females, 78 [19.1%] males) were assigned to the depressive symptoms group. There were significant differences between the non-depressive symptoms and depressive symptoms groups in the NLR [median (25th to 75th percentile): 1.54 (1.24, 1.97) vs. 1.76 (1.32, 2.37), P = 0.005] and the PLR [median (25th to 75th percentile): 123.7 (102.0, 153.9) vs. 136.8 (107.0, 166.5), P = 0.047] in males, but not in females. The multivariate logistic regression analysis demonstrated that the NLR was significantly associated with depressive symptoms in males (adjusted odds ratio: per 1 increase, 1.570; 95% confidence interval: 1.120–2.220; P = 0.009). In conclusion, our findings indicate that higher NLR may be associated with depressive symptoms in males.
Detection of SARS-CoV-2 in subcutaneous fat but not visceral fat, and the disruption of fat lymphocyte homeostasis in both fat tissues in the macaque
Authors: Olivo Anaëlle , Marlin Romain , Lazure Thierry , Maisonnasse Pauline , Bossevot Laetitia , Mouanga Christelliah , Lemaitre Julien , Pourcher Guillaume , Benoist Stéphane , Le Grand Roger , Lambotte Olivier , Dereuddre-Bosquet Nathalie , Bourgeois Christine
Published: June 2022

The well documented association between obesity and the severity of SARS-CoV-2 infection raises the question of whether adipose tissue (AT) is impacted during this infection. Using a model of SARS-CoV-2 infection in cynomolgus macaques, we detected the virus within subcutaneous AT (SCAT) but not in visceral AT (VAT) or epicardial AT on day 7 post-infection. We sought to determine the mechanisms responsible for this selective detection and observed higher levels of angiotensin-converting-enzyme-2 mRNA expression in SCAT than in VAT. Lastly, we evaluated the immunological consequences of SARS-CoV-2 infection on AT: both SCAT and VAT T cells showed a drastic reduction in CD69 expression, a standard marker of resident memory T cell in tissue, that is also involved in the migratory and metabolic properties of T cells. Our results demonstrate that in a model of mild infection, SCAT is selectively infected by SARS-CoV-2 although changes in the immune properties of AT are observed in both SCAT and VAT.
A qualitative analysis method for multi-component gas mixtures via terahertz rotational spectroscopy
Authors: Li Jia , Deng Xiaojiao , Zheng Xiaoping , Ren Yimin
Published: June 2022

A novel qualitative analysis method for gas mixtures based on the collision broadening mechanism takes advantage of the terahertz rotational spectrum.
Antimicrobial mechanisms of biomaterials: from macro to nano
Authors: Roy Shounak , Sarkhel Sanchita , Bisht Deepali , Hanumantharao Samerender Nagam , Rao Smitha , Jaiswal Amit
Published: June 2022

This review provides a comprehensive understanding about how different classes of biomaterials, starting from macroscale to nanoscale, interact with bacteria and viruses to exert their antimicrobial properties through diverse mechanisms of action.
Adsorption of NO, NO2 and H2O in divalent cation faujasite type zeolites: a density functional theory screening approach
Authors: Daouli Ayoub , Hessou Etienne Paul , Monnier Hubert , Dziurla Marie-Antoinette , Hasnaoui Abdellatif , Maurin Guillaume , Badawi Michael
Published: June 2022

We show by DFT calculations that emissions of NOx in confined work environments could be mitigated by PtY zeolite.
Enhanced luminescence of tris(carboxylato)uranyl(vi) complexes and energy transfer to Eu(iii): a combined spectroscopic and theoretical investigation
Authors: Kumar Satendra , Maji S. , Sundararajan K.
Published: June 2022

Enhanced luminescent tris(carboxylato) uranyl(vi) complexes are reported. In a very dilute mixture of [UO2(L)3]− and Eu(iii), energy transfer from uranyl to Eu(iii) is observed.
Coupled impact of proteins with different molecular weights and surface charges on TiO2 mobility
Authors: Yan Chaorui , Sharma Prabhakar , Chen Qing , Li Baoguo , Shang Jianying
Published: June 2022

The widely present proteins in the natural environment interact with released nanoparticles, which changes the stability, transport, and fate of nanoparticles.
The novel anti-neuroinflammatory functional food CCL01, a mixture of Cuscuta seed extracts and Lactobacillus paracasei NK112
Authors: Hong Seong Min , Ju In Gyoung , Kim Jin Hee , Park Sang Cheol , Choi Jin Gyu , Sohn Mi Won , Oh Myung Sook , Kim Sun Yeou
Published: June 2022

The medical herbal mixture (CCL01) including Cuscuta seeds and Lactobacillus paracasei NK112 has inhibitory effect on LPS-induced microglial inflammation and may be a potential functional food for neuroinflammation-related diseases.
Recent applications of covalent chemistries in protein–protein interaction inhibitors
Authors: Chan Alexandria M. , Goodis Christopher C. , Pommier Elie G. , Fletcher Steven
Published: June 2022

Often, large molecules are required to effectively disrupt protein–protein interactions (PPIs). Exploiting covalent chemistries may realize potent therapeutics boasting more “drug-like” properties with longer residence times.
Efficient Cr3+-activated NaInP2O7 phosphor for broadband near-infrared LED applications
Authors: Wang Qi , Wang Shangwei , Tan Tao , Wang Jiutian , Pang Ran , Li Da , Li Chengyu , Zhang Hongjie
Published: June 2022

A novel efficient broadband NIR phosphor NaInP2O7:Cr3+ (in brief NIP:Cr3+) for NIR pc-LEDs has been successfully synthesized and systematically investigated.
Ontological orientations of educators’ sense of the atom and underlying source domains: a case study of Kotebe Metropolitan University, Ethiopia
Authors: Lemma Abayneh , Belachew Woldie
Published: June 2022

This study aimed to uncover the ontological orientations of educators’ sense of the atom of Kotebe Metropolitan University (KMU), Ethiopia. Thus, an interpretative case study design was employed, with the analytic approach of grounded theory, due to the importance of atomic ontology as a case and the need for an in-depth analysis of their thinking and possible interpretations. The data were collected through a semi-structured interview of eight chemistry educators of the university and coded using Charmaz's approach to coding. The History and Philosophy of Science (HPS) was employed for identifying and sorting the data into the ontological categories, while the theory of experientialism (ToE) was used for locating source domains. Consequently, the interpretative ontological view was traced with two atomic notions: the functional and chemical atomic notions. This means that the educators do believe in the existence of the atom in the interpretative way in which their knowledge is well informed by interpretations and inferences of the experimental evidence and applications of quantum mechanics. However, their narrative lacks some essential priori philosophical arguments and scientific contexts and evidence. As a result, this ‘the latest is the best’ thinking pattern was traced as a major source domain to which the interpretative orientation is attributed.
Highly selective acid-catalyzed olefin isomerization of limonene to terpinolene by kinetic suppression of overreactions in a confined space of porous metal–macrocycle frameworks
Authors: He Wei , Tashiro Shohei , Shionoya Mitsuhiko
Published: June 2022

A porous metal–macrocycle framework (MMF) equipped with a Brønsted acid catalyst in nanochannels enables highly selective isomerization of limonene to terpinolene by kinetically suppressing over-isomerization at confined acid sites.
Gramicidin A accumulates in mitochondria, reduces ATP levels, induces mitophagy, and inhibits cancer cell growth
Authors: Xue Yun-Wei , Itoh Hiroaki , Dan Shingo , Inoue Masayuki
Published: June 2022

Here we revealed the spatiotemporal behavior of gramicidin A in cancer cells. Gramicidin A depolarizes both the plasma and mitochondrial membranes, inhibits ATP synthesis, and induces mitophagy, thereby causing potent inhibition of cell growth.
Effect of structure on oxygen diffusivity in layered oxides: a combined theoretical and experimental study
Authors: Kim ChangSub , Park Kyoung-Won , Kalaev Dmitri , Nicollet Clement , Tuller Harry L.
Published: June 2022

Effect of structure on oxygen diffusion D0 in two different structures of La2CuO4. Higher density of mobile defects in T-structure leads to higher D0, but the lower activation energy for T′-structure is correlated with its lower migration energy.