bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-cell transcriptomic analysis reveals tumor cell heterogeneity and immune microenvironment features of pituitary neuroendocrine tumors
Authors: Yan Nan , Xie Weiyan , Wang Dongfang , Fang Qiuyue , Guo Jing , Chen Yiyuan , Li Xinqi , Gong Lei , Wang Jialin , Guo Wenbo , Zhang Xuegong , Zhang Yazhuo , Gu Jin , Li Chuzhong
Published: January 2024

Background

Pituitary neuroendocrine tumors (PitNETs) are one of the most common types of intracranial tumors. Currently, the cellular characteristics of normal pituitary and various other types of PitNETs are still not completely understood.

Methods

We performed single-cell RNA sequencing (scRNA-seq) on 4 normal samples and 24 PitNET samples for comprehensive bioinformatics analysis. Findings regarding the function ofPBKin the aggressive tumor cells were validated by siRNA knockdown, overexpression, and transwell experiments.

Results

We first constructed a reference cell atlas of the human pituitary. Subsequent scRNA-seq analysis of PitNET samples, representing major tumor subtypes, shed light on the intrinsic cellular heterogeneities of the tumor cells and tumor microenvironment (TME). We found that the expression of hormone-encoding genes defined the major variations of the PIT1-lineage tumor cell transcriptomic heterogeneities. A sub-population of TPIT-lineage tumor cells highly expressingGZMKsuggested a novel subtype of corticotroph tumors. In immune cells, we found two clusters of tumor-associated macrophages, which were both highly enriched in PitNETs but with distinct functional characteristics. In PitNETs, the stress response pathway was significantly activated in T cells. While a majority of these tumors are benign, our study unveils a common existence of aggressive tumor cells in the studied samples, which highly express a set of malignant signature genes. The following functional experiments confirmed the oncogenic role of selected up-regulated genes. The over-expression ofPBKcould promote both tumor cell proliferation and migration, and it was also significantly associated with poor prognosis in PitNET patients.

Conclusions

Our data and analysis manifested the basic cell types in the normal pituitary and inherent heterogeneity of PitNETs, identified several features of the tumor immune microenvironments, and found a novel epithelial cell sub-population with aggressive signatures across all the studied cases.
Inhibitory effects of Acanthopanax sessiliflorus Harms extract on the etiology of rheumatoid arthritis in a collagen-induced arthritis mouse model
Authors: Kim Dahye , Heo Yunji , Kim Mangeun , Suminda Godagama Gamaarachchige Dinesh , Manzoor Umar , Min Yunhui , Kim Minhye , Yang Jiwon , Park Youngjun , Zhao Yaping , Ghosh Mrinmoy , Son Young-Ok
Published: January 2024

Background

The biological function of Acanthopanax sessiliflorus Harm (ASH) has been investigated on various diseases; however, the effects of ASH on arthritis have not been investigated so far. This study investigates the effects of ASH on rheumatoid arthritis (RA).

Methods

Supercritical carbon dioxide (CO2) was used for ASH extract preparation, and its primary components, pimaric and kaurenoic acids, were identified using gas chromatography-mass spectrometer (GC–MS). Collagenase-induced arthritis (CIA) was used as the RA model, and primary cultures of articular chondrocytes were used to examine the inhibitory effects of ASH extract on arthritis in three synovial joints: ankle, sole, and knee.

Results

Pimaric and kaurenoic acids attenuated pro-inflammatory cytokine-mediated increase in the catabolic factors and retrieved pro-inflammatory cytokine-mediated decrease in related anabolic factors in vitro; however, they did not affect pro-inflammatory cytokine (IL-1β, TNF-α, and IL-6)-mediated cytotoxicity. ASH effectively inhibited cartilage degradation in the knee, ankle, and toe in the CIA model and decreased pannus development in the knee. Immunohistochemistry demonstrated that ASH mostly inhibited the IL-6-mediated matrix metalloproteinase. Gene Ontology and pathway studies bridge major gaps in the literature and provide insights into the pathophysiology and in-depth mechanisms of RA-like joint degeneration.

Conclusions

To the best of our knowledge, this is the first study to conduct extensive research on the efficacy of ASH extract in inhibiting the pathogenesis of RA. However, additional animal models and clinical studies are required to validate this hypothesis.
Proteomic profiling identifies SPP1 associated with rapidly progressive interstitial lung disease in anti-MDA5-positive dermatomyositis
Authors: Qiu Yulu , Feng Xiaoke , Liu Chang , Shi Yumeng , Xu Lingxiao , You Hanxiao , Wang Lei , Lv Chengyin , Wang Fang , Tan Wenfeng
Published: January 2024

Background

Anti-melanoma differentiation-associated gene five antibody positive (MDA5+) dermatomyositis (DM) is significantly associated with rapidly progressive interstitial lung disease (RP-ILD). Early detection of RP-ILD remains a major challenge. This study aims to identify and validate prognostic factors for RP-ILD in MDA5+ DM patients.

Methods

Plasma samples from 20 MDA5+ DM patients and 10 healthy controls (HC) were collected for proteomic analysis using liquid chromatography-tandem mass spectrometry (LC–MS/MS) analysis. The proteins of interest were validated in independent samples (20 HC, 20 MDA5+ DM with RP-ILD, and 20 non-RP-ILD patients) with enzyme-linked immunosorbent assay (ELISA).

Results

A total of 413 differentially expressed proteins (DEPs) were detected between the MDA5+ DM patients and HC. When comparing DEPs between RP-ILD and non-RP-ILD patients, 79 proteins were changed in RP-ILD patients, implicating acute inflammatory response, coagulation, and complement cascades. Six candidate biomarkers were confirmed with ELISA. Secreted phosphoprotein 1 (SPP1), serum amyloid A1 (SAA1), and Kininogen 1 (KNG1) concentrations were significantly elevated in RP-ILD patients than those in non-RP-ILD patients and HC. In the different clinical subgroups, SPP1 was particularly elevated in the high-risk RP-ILD subgroup of MDA5+ DM.

Conclusion

This study provides novel insights into the pathogenesis of RP-ILD development in MDA5+ DM and suggests the plasma protein SPP1 could serve as a potential blood biomarker for RP-ILD early warning.
Autoimmune congenital heart block: a case report and review of the literature related to pathogenesis and pregnancy management
Authors: Huang Ying , Deng Jialin , Liu Jinghua , Yang Fangyuan , He Yi
Published: January 2024

Autoimmune congenital heart block (ACHB) is a passively acquired immune-mediated disease characterized by the presence of maternal antibodies against components of the Ro/SSA and La/SSB ribonucleoprotein complex that mainly affects the cardiac conducting system. ACHB occurs in 2% of women with positive anti-Ro/SSA and anti-La/SSB antibodies and causes a high risk of intrauterine fetal death, neonatal mortality, and long-term sequelae. In this review, we first describe a case of ACHB to provide preliminary knowledge. Then, we discuss the possible pathogenic mechanisms of ACHB; summarize the pregnancy management of patients with positive anti-Ro/SSA and anti-La/SSB antibodies and/or rheumatic diseases, the prevention of ACHB, and the treatment of ACHB fetuses; and propose routine screening of these antibodies for the general population. Careful follow-up, which consists of monitoring the fetal heart rate, is feasible and reassuring for pregnant women with positive anti-Ro/SSA and/or anti-La/SSB antibodies to lower the risk of ACHB in fetuses. Moreover, maternal administration of hydroxychloroquine may be useful in preventing ACHB in pregnant women with anti-Ro/SSA and/or anti-La/SSB antibodies.
The relationship between the gut microbiome and resistance training: a rapid review
Authors: Wagner Adam , Kapounková Kateřina , Struhár Ivan
Published: January 2024

The human gut microbiome is attracting increasing attention because of its overall effect on health. Several reviews have investigated the impact of physical activity on the gut microbiome; however, these predominantly concentrate on either endurance or a combination of physical activities. This study aims to describe the effect of resistance or strength training on the gut microbiome of a human population. This rapid review follows the guidelines of the Cochrane Rapid Reviews Guidance along with PRISMA. A review of the literature was carried out using articles indexed by PubMed, Scopus, and Web of Science published in the last 12 years. None of the seven studies included find significant change in the gut microbiome in terms of bacterial taxa composition or overall diversity, though the results show that resistance training might decrease the zonulin level and increase mucin production and thereby reduce inflammation in the gut. Interestingly, two studies point to a gut-muscle axis connection and this is discussed in our paper. However, due to the small number of existing studies and certain methodological disagreements, it was hard to find a consensus on the relationship between the gut microbiome and resistance training.
The physiological and physical benefits of two types of concurrent training: a randomized controlled trial
Authors: Canli Umut , Aldhahi Monira I.
Published: January 2024

Background

It is widely acknowledged that aerobic exercise and strength training are crucial components of most workout programs. However, there is no consensus as to whether the effectiveness of exercises is affected by the sequence in which they are performed. Therefore, the overarching aim of the study was to understand the optimal order of two types of concurrent training program for 13 weeks by comparing the effectiveness of the training on body composition, predicated maximal oxygen uptake (VO2max), dynamic respiratory parameters and muscle strength in healthy middle-aged people.

Methods

Thirty-three middle-aged individuals, who were categorized as moderately active based on their responses to International Physical Activity Questionnaires, underwent random allocation. The participants were randomly assigned into two groups: the Strength Training followed by Aerobic Training group (SAG, n = 16) and the Aerobic Training followed by Strength Training group (ASG, n = 17). Body composition, aerobic endurance, respiratory parameters, and upper and lower strength were assessed at baseline and after (post-test) a 13-week intervention. The chi-square test and the independent t-test were used to compare sociodemographic variables between the groups. A 2 × 2 analysis of variance (ANOVA) with repeated measures (group x measurement) was conducted. The study was retrospectively registered on clinicaltrials.gov in May of 2023 (clinicaltials.gov identifier: NCT05862415; in 04/25/2023).

Results

Findings showed no significant differences between the group in the VO2max, FVC or FEV1 (F = 1.122, 0.028, 0.06, 2.483; p > 0.05, respectively). Intragroup analysis revealed changes in PEF compared to baseline in the ASG (F = 5.895; p < 0.05). Increases were observed in all strength parameters for both training programs.

Conclusions

The concurrent training effect on muscle composition, oxygen consumption and muscle strength specifically 1RM, in middle-aged individuals are equivocal, regardless of the exercise order. The results indicate that both exercise sequences can elicit similar benefits in terms of cardiovascular fitness, muscular strength, and endurance. This lack of difference suggests that the order of exercise does not play a significant role in determining the effectiveness of the workout or the subsequent physiological adaptations.

Clinicaltials.gov identifier

NCT05862415. Date of registration: 04/25/2023
Hypothesized mechanisms of death in swimming: a systematic review
Authors: Yao Yunheng , DiNenna Michael A. , Chen Lili , Jin Shirong , He Sixian , He Jinshen
Published: January 2024

Background

The study aims to update the specific classification of mechanisms of death in swimming and to demonstrate these categories are reasonable, by analyzing more characteristics of death cases, evaluating the available evidence and determining their quality.

Methods

Original articles were queried from PubMed, Web of Science, Embase databases, Cochrane Library, and Scopus. Included studies, which were evaluated as level 4 evidence or higher according to the Oxford Centre for Evidence-Based Medicine, discussed hypothesized mechanisms of death in swimming. Parameters analyzed in this study included decedents’ characteristics, outcome measures, findings, methodological index for non-randomized studies (MINORS), and critical evaluation of each study classified by death mechanism.

Results

A total of twenty-five studies were included for further analysis: fourteen were associated with cardiovascular diseases, two were about cerebrovascular diseases, two contained respiratory diseases, seven were about hazardous conditions and three contained other drownings, which provided evidence for mechanisms of death.

Conclusions

It is found that cardiovascular disease is the main cause or contributing factor of death in swimming. Respiratory diseases and cerebrovascular diseases are difficult to be definitive mechanism categories due to insufficient evidence. Hazardous conditions appear to be one of the possible risk factors because there are more cases of deaths from unsafe environments in swimming, but further statistics and research are still needed to support this view. Our study may have important implications for developing potential prevention strategies for sports and exercise medicine.

Trial registration

PROSPERO ID (CRD42021267330). Registered Aug 13th 2021.
Phyllosphere bacteria with antiquorum sensing and antibiofilm activities against fish pathogenic bacteria
Authors: Lukman Griselda , Waturangi Diana Elizabeth , Julyantoro Pande Gde Sasmita , Papuangan Nurmaya
Published: January 2024

Objective

This research aims to quantify antiquorum sensing and antibiofilm activity of f phyllosphere bacteria against biofilm formed by pathogenic fish bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi.

Results

Antiquorum sensing assay using Chromobacter violaceum as indicator bacteria and antibiofilm assay showed six phyllosphere bacteria have antiquorum sensing and antibiofilm activities against tested bacteria. The highest inhibition and destruction activity was showed by metabolite of JB 3B and EJB 5 F against A. hydrophila, respectively. Determination using light microscope and scanning electron microscope performed decreaing in biomass of biofilm observed after treated with metabolite from phyllosphere bacteria.
Proteome dataset of Candida albicans (ATCC10231) opaque cell
Authors: Zore Gajanan , Abdulghani Mazen , Kodgire Santosh , Kazi Rubina , Shelar Amruta , Patil Rajendra
Published: January 2024

Objectives

Candida albicans, a polymorphic yeast, is one of the most common, opportunistic fungal pathogens of humans. Among the different morphological forms, opaque form is one of the least-studied ones. This opaque phenotype is essential for mating and is also reported to be involved in colonizing the gastrointestinal tract. Considering the significance of the clinical and sexual reproduction of C. albicans, we have investigated the morphophysiological modulations in opaque form using a proteomic approach.

Data description

In the current investigation, we have used Micro-Liquid Chromatography-Mass Spectrometry (LC-MS/MS) analysis to create a protein profile for opaque-specific proteins. Whole-cell proteins from C. albicans (ATCC10231) cells that had been cultured for seven days on synthetic complete dextrose (SCD) medium in both as an opaque (test) and as a white (control) form cells were extracted, digested, and identified using LC-MS/MS. This information is meant to serve the scientific community and represents the proteome profile (SWATH Spectral Libraries) of C. albicans opaque form.
Prevalence of dietary supplement use among male Brazilian recreational triathletes: a cross-sectional study
Authors: de Oliveira Vinnycius Nunes , Andrade Marilia Santos , Sinisgalli Rafaella , Vancini Rodrigo Luiz , de Conti Teixeira Costa Gustavo , Weiss Katja , Knechtle Beat , de Lira Claudio Andre Barbosa
Published: January 2024

Background and rationale

The literature shows that the prevalence of dietary supplements is high and guidance by a nutritionist or specialized professional is low in professional triathletes. It is reasonable to assume that in recreational triathletes, the prevalence of dietary supplements will also be high and that a significant portion of the sampled population will use supplements without any guidance from a qualified professional. The present study investigated dietary supplement use among Brazilian male recreational triathletes.

Methods

A total of 724 Brazilian male recreational triathletes (age: 38.00 [10.00] years and body mass index: 24.16 [3.02] kg/m2) took part in this study. All participants answered an online questionnaire containing questions about their demographic characteristics and the nutritional aspects of their diet.

Results

The results showed that ~ 90% (n = 653) of the interviewed participants reported using at least one dietary supplement. Surprisingly, ~ 25% did not receive supplement advice from a professional nutritionist.

Conclusion

The prevalence of dietary supplements in male recreational triathletes was high, and a substantial part of the sample did not receive professional recommendations. This situation is worrisome because dietary supplements should be prescribed by a professional nutritionist.

Practical implications

Our results suggest the need for an appropriate attitude and guidance by health professionals who deal with this population, especially nutritionists, to promote safe practices.
Examining the influence of wealth status on prehypertension risk in women aged 30–49: evidence from the 2018 Benin demographic and health survey
Authors: Ayebeng Castro , Okyere Joshua , Salu Samuel , Dickson Kwamena Sekyi
Published: January 2024

Background

There is an interest in the extent to which the wealth status of women predicts their risk of being pre-hypertensive. This understanding is lacking in the current body of empirical literature, particularly within the context of Benin. Thus, indicating a knowledge gap that must be filled. To this end, the present study aimed to assess the association between wealth status and the risk of prehypertension among women aged 30–49 years in Benin.

Methods

This study used a secondary data from the recent (2018) Demographic and Health Survey of Benin. Bivariate and multivariate logistic regression models were computed to examine the association between wealth index and the risks of prehypertension using Stata version 14. Findings were presented in adjusted odds ratio at 95% confidence level.

Results

Women in the richest wealth index were significantly more likely to have prehypertension than those in the poorest wealth index [AOR = 1.4; 95%CI: 1.26–2.26]. Women aged 45–49 years were more likely to have prehypertension [AOR = 1.5; 95%CI: 1.15–1.98] compared to younger women. Women who used unclean cooking fuel were less likely to have prehypertension compared to those using clean cooking fuel [AOR = 0.6; 95%CI: 0.37,0.87].

Conclusion

The study concludes that wealth status is a significant predictor of prehypertension among women in Benin. Therefore, the Ministry of Health in Benin should prioritize health education and prehypertension awareness campaigns specifically targeting women in affluent communities and households. These campaigns should focus on promoting healthy dietary choices and encouraging physical activity to mitigate the elevated risk associated with wealth status. Recognizing the influence of age on prehypertension risk, it is imperative for older-middle aged women to be targeted as primary beneficiaries of health education programs and prehypertension screening programs.
The fluorescent ligand bTVBT2 reveals increased p-tau uptake by retinal microglia in Alzheimer’s disease patients and AppNL−F/NL−F mice
Authors: Nuñez-Diaz Cristina , Andersson Emelie , Schultz Nina , Pocevičiūtė Dovilė , Hansson Oskar ,   The Netherlands Brain Bank, Nilsson K Peter R. , Wennström Malin
Published: January 2024

Background

Amyloid beta (Aβ) deposits and hyperphosphorylated tau (p-tau) accumulation have been identified in the retina of Alzheimer’s disease (AD) patients and transgenic AD mice. Previous studies have shown that retinal microglia engulf Aβ, but this property decreases in AD patients. Whether retinal microglia also take up p-tau and if this event is affected in AD is yet not described. In the current study, we use the p-tau-specific thiophene-based ligand bTVBT2 to investigate the relationship between disease progression and p-tau uptake by microglia in the retina of AD patients and AppNL−F/NL−F knock-in mice, an AD mouse model known to demonstrate extracellular Aβ plaques and dystrophic neurites in the brain from 6 months of age.

Methods

Evaluation of bTVBT2 specificity and its presence within microglia was assessed by immunofluorescent staining of hippocampal sections and flat-mount retina samples from non-demented controls, AD patients, 3-, 9-, and 12-month-old AppNL−F/NL−F knock-in mice and 12- and 18-month-old wild type (WT) mice. We used ImageJ to analyze the amount of bTVBT2 inside Iba1-positive microglia. Co-localization between the ligand and p-tau variant Ser396/Ser404 (PHF-1), Aβ, phosphorylated TAR DNA binding protein 43 (pTDP-43), and islet amyloid polypeptide (IAPP) in the brain and retina was analyzed using confocal imaging.

Results

Confocal imaging analysis showed that bTVBT2 binds to PHF-1- and AT8-positive aggregates inside retinal microglia, and not to Aβ, pTDP-43, or IAPP. The density of bTVBT2-positive microglia was higher in cases with a high Aβ load compared to those with a low Aβ load. This density correlated with the neurofibrillary tangle load in the brain, but not with retinal levels of high molecular weight (aggregated) Aβ40 or Aβ42. Analysis of AppNL−F/NL−F knock-in mouse retina further showed that 50% of microglia in 3-month-old AppNL−F/NL−F knock-in mice contained bTVBT2. The percentage significantly increased in 9- and 12-month-old mice.

Conclusion

Our study suggests that the microglial capability to uptake p-tau in the retina persists and intensifies with AD progression. These results also highlight bTVBT2 as a ligand of interest in future monitoring of retinal AD pathology.
IL-1β and iNOS can drive the asthmatic comorbidities and decrease of lung function in perennial allergic rhinitis children
Authors: Han Myung Woul , Kim Song Hee , Oh Inbo , Kim Yang ho , Lee Jiho
Published: January 2024

Background

Allergic asthma and rhinitis (AR) are closely linked, with a significant proportion of AR patients developing asthma. Identification of the early signs of comorbidity of AR and asthma can enable prompt treatment and prevent asthma progression.

Objectives and methods

This study investigated the role of interleukin-1β (IL-1β), a pro-inflammatory cytokine, and inducible nitric oxide synthase (iNOS) in the comorbidity of AR and asthma and lung function in Korean children with perennial AR (PAR). A cohort of 240 subjects (6 to 10 years old) with PAR (PAR alone: 113 children, PAR and asthma: 127 children) was analyzed for various biomarkers, including IL-1β, iNOS, and epithelial-mesenchymal transition (EMT) markers in serum. The blood levels of eosinophils and immunoglobulin E (IgE) were examined. IL-1β, CCL-24, E-cadherin, and vimentin were measured by enzyme-linked immunosorbent assay (ELISA). Epithelial iNOS was measured by the NOS kit.

Results

Elevated levels of IL-1β, iNOS, and vimentin in the serum were identified as significant indicators of the likelihood of comorbidity of PAR and asthma in children. Furthermore, higher concentrations of IL-1β, iNOS, and vimentin have been linked to reduced lung function in PAR children. Notably, IL-1β expression shows a relationship with the levels of E-cadherin, vimentin, and CCL-24. However, no correlation was found between IL-1β and iNOS expressions.

Conclusions

This study suggests that IL-1β and iNOS can be biomarkers in the progression of PAR and asthma and decreased lung function, suggesting potential targets for early intervention and treatment.
Clinical characteristics of pneumothorax and pneumomediastinum in mechanical ventilated patients with coronavirus disease 2019: a case series
Authors: Ide Yohei , Urushibata Nao , Takayama Wataru , Hondo Kenichi , Aiboshi Junichi , Otomo Yasuhiro
Published: January 2024

Background

Pneumothorax (PTX) and pneumomediastinum (PM) have been reported as potential complications in patients with coronavirus disease 2019 (COVID-19); however, their risk factors and etiology remain unknown. Herein, we investigated the clinical characteristics of mechanically ventilated patients with COVID-19 with PTX or PM.

Methods

We examined patients with severe COVID-19 requiring mechanical ventilation who were admitted to the intensive care unit of a tertiary-level emergency medical center in Tokyo, Japan between April 1, 2020. and October 31, 2021. We collected and analyzed the clinical characteristics of the patients who presented with either PTX or PM during mechanical ventilation.

Results

During the study period, a total of 165 patients required mechanical ventilation, and 15 patients with PTX/PM during mechanical ventilation were selected. Three patients with obvious causes were excluded, and the remaining 12 patients were analyzed (7.3%). The mortality rate in these patients was as high as 50%, demonstrating the difficulty of treatment in the presence of PTX/PM. PTX/PM occurred 14.5 days after intubation. A peak pressure of > 30 cmH2O was only apparent in one patient, suggesting that high positive pressure ventilation may be less involved than mentioned in the literature. In addition, the inspiratory effort was not strong in our group of patients. (P0.1 was 2.1 cm H2O [1.0–3.8]).

Conclusion

Various factors are associated with the development of PTX/PM in patients on mechanical ventilation for COVID-19. We did not find a strong correlation between PTM/PM and barotrauma or strong inspiratory efforts, which have been identified as potential causes in previous studies.
A short 18F-FDG imaging window triple injection neuroimaging protocol for parametric mapping in PET
Authors: Moradi Hamed , Vashistha Rajat , O’Brien Kieran , Hammond Amanda , Vegh Viktor , Reutens David
Published: January 2024

Background

In parametric PET, kinetic parameters are extracted from dynamic PET images. It is not commonly used in clinical practice because of long scan times and the requirement for an arterial input function (AIF). To address these limitations, we designed an 18F-fluorodeoxyglucose (18F-FDG) triple injection dynamic PET protocol for brain imaging with a standard field of view PET scanner using a 24-min imaging window and an input function modeled using measurements from a region of interest placed over the left ventricle.

Methods

To test the protocol in 6 healthy participants, we examined the quality of voxel-based maps of kinetic parameters in the brain generated using the two-tissue compartment model and compared estimated parameter values with previously published values. We also utilized data from a 36-min validation imaging window to compare (1) the modeled AIF against the input function measured in the validation window; and (2) the net influx rate ($$K_{i}$$

K
i

) computed using parameter estimates from the short imaging window against the net influx rate obtained using Patlak analysis in the validation window.

Results

Compared to the AIF measured in the validation window, the input function estimated from the short imaging window achieved a mean area under the curve error of 9%. The voxel-wise Pearson’s correlation between $$K_{i}$$

K
i

estimates from the short imaging window and the validation imaging window exceeded 0.95.

Conclusion

The proposed 24-min triple injection protocol enables parametric 18F-FDG neuroimaging with noninvasive estimation of the AIF from cardiac images using a standard field of view PET scanner.
Upcoding in medicare: where does it matter most?
Authors: Joiner Keith A. , Lin Jianjing , Pantano Juan
Published: January 2024

Upcoding in Medicare has been a topic of interest to economists and policy makers for nearly 40 years. While upcoding is generally understood as “billing for services at higher level of complexity than the service actually pro- vided or documented,” it has a wide range of definitions within the literature. This is largely because the financial incentives across programs and aspects under the coding control of billing specialists and providers are different, and have evolved substantially over time, as has the published literature. Arguably, the primary importance of analyzing upcoding in different parts of Medicare is to inform policy makers on the magnitude of the process and to suggest approaches to mitigate the level of upcoding. Financial estimates for upcoding in traditional Medicare (Medicare Parts A and B), are highly variable, in part reflecting differences in methodology for each of the services covered. To resolve this variability, we used summaries of audit data from the Comprehensive Error Rate Testing program for the period 2010–2019. This program uses the same methodology across all forms of service in Medicare Parts A and B, allowing direct comparisons of upcoding magnitude. On average, upcoding for hospitalization under Part A represents $656 million annually (or 0.53% of total Part A annual expenditures) during our sample period, while up- coding for physician services under Part B is $2.38 billion annually (or 2.43% of Part B annual expenditures). These numbers compare to the recent consistent estimates from multiple different entities putting upcoding in Medicare Part C at $10–15 billion annually (or approximately 2.8–4.2% of Part C annual expenditures). Upcoding for hospitalization under Medicare Part A is small, relative to overall upcoding expenditures.
Cost-effectiveness analysis of parenteral iron therapy compared to oral iron supplements in managing iron deficiency anemia among pregnant women
Authors: Saha Somen , Raval Devang , Shah Komal , Saxena Deepak
Published: January 2024

Objective

This study compared the clinical efficacy and cost-effectiveness of parenteral iron, using intravenous iron sucrose (IVIS) therapy against the standard regimen of oral iron (OI) therapy for managing iron-deficiency anemia (IDA) among pregnant women in a natural primary care setting in Gujarat.

Design

A prospective cost-effectiveness study was conducted in natural programme setting wherein 188 pregnant women in their 14 to 18 weeks with moderate and severe anemia women enrolled from two districts of Gujarat, and 142 were followed up until the post-partum phase. The intervention group comprised of 82 participants who were administered IVIS, while the comparison group comprised of 106 participants who were put on OI therapy. Hemoglobin (Hb) levels were measured at periodic intervals, first during enrollment and then during each month of pregnancy period and finally on the 42nd day of the post-natal period.

Outcome measures

Change in mean Hb level from baseline was the primary outcome, while the incidence of morbidity and mortality was a secondary outcome measure.

Results

The intervention group showed a significant incremental mean change in Hb level from 8.2 g/dl to 11.45 g/dl at the fourth follow-up, while the control group's mean Hb level reduced from 9.99 g/dl to 9.55 g/dl. The discounted cost per beneficiary for IVIS was US$ 87, while that for OI was US$ 49. The incremental cost-effectiveness ratio (ICER) was US$ 9.84, which is 0.049% of India's per capita GDP.

Conclusion

IVIS therapy was more clinically effective and cost-effective than OI therapy among pregnant women for management of moderate and severe anemia.
Forest management and former land use have no effect on soil fungal diversity in uneven-aged mountain high forests
Authors: Mollier Sylvain , Kunstler Georges , Dupouey Jean-Luc , Mulero Stephen , Bergès Laurent
Published: January 2024

Key message

Metabarcoding analysis of soil fungal communities in French mountain forests revealed that harvesting intensity, time since last harvest and former land use had no effect on fungal community composition compared to key abiotic factors. Low-intensity management in these uneven-aged mountain forests therefore has limited effects on soil fungal community composition which is mainly driven by elevation and edaphic properties.

Context

Past and current human activities are known to affect forest biodiversity. However, the effects of former land use and forest management have been studied much more extensively on higher plants than on fungi.

Aims

Our objectives were to assess the effects of harvesting intensity, duration since last harvest and former land use on soil fungal communities in uneven-aged mountain high forests.

Methods

On the basis of historical land-use maps drawn between 1862 and 1864 and on historical forest management archives, we selected 62 sites in the French Alps with contrasting land-use histories (ancient forests, which were already forested on historical maps vs recent forests, which have recovered following abandonment of pastures) and different durations since last harvest (from 1 to over 50 years). We carried out soil sampling and assessed fungal diversity by metabarcoding analysis. We analysed soil fungal molecular operational taxonomic units (MOTU) diversity as a whole and for the main lifestyle groups (such as wood saprotrophic or ectomycorrhizal fungi) using multiple linear regressions on Shannon’s diversity index and fungal taxonomic composition using canonical correlation analysis.

Results

We found no significant effect of harvesting intensity, time since last harvest or land-use history on total fungal MOTU diversity, fungal lifestyle diversity or taxonomic composition. In contrast, we observed significant effects of elevation, pH, organic carbon and available phosphorus content on the taxonomic and functional composition of soil fungal communities.

Conclusions

The structure of soil fungal communities (i.e. diversity and species composition) was mainly determined by elevation and edaphic factors, indicating a high-context dependency, as previously found in similar studies. Our study in mountain forests shows that recent forests established on former pastures had no legacy effect on soil conditions and fungal communities, in contrast to previous results in lowland areas, where recent forests were mainly established on former cropland. Uneven-aged forest management had no effect on fungal diversity, in contrast to previous results observed in even-aged high forests.
An experimental study of neural estimators of the mutual information between random vectors modeling power spectrum features
Authors: Shin Donghoon , Kim Hyung Soon
Published: January 2024

Mutual information (MI) quantifies the statistical dependency between a pair of random variables and plays a central role in signal processing and data analysis. Recent advances in machine learning have enabled the estimation of MI from a dataset using the expressive power of neural networks. In this study, we conducted a comparative experimental analysis of several existing neural estimators of MI between random vectors that model power spectrum features. We explored alternative models of power spectrum features by leveraging information-theoretic data processing inequality and bijective transformations. Empirical results demonstrated that each neural estimator of MI covered in this study has its limitations. In practical applications, we recommend the collective use of existing neural estimators in a complementary manner for the problem of estimating MI between power spectrum features.
A multi-task learning speech synthesis optimization method based on CWT: a case study of Tacotron2
Authors: Hu Guoqiang , Ruan Zhuofan , Guo Wenqiu , Quan Yujuan
Published: January 2024

Text-to-speech synthesis plays an essential role in facilitating human-computer interaction. Currently, the predominant approach in Text-to-speech acoustic models selects only the Mel spectrum as an intermediate feature for converting text to speech. However, the Mel spectrograms obtained may exhibit ambiguity in some aspects owing to the limited capability of the Fourier transform to capture mutation signals during the acquisition of the Mel spectrograms. With the aim of improving the clarity of synthesized speech, this study proposes a multi-task learning optimization method and conducts experiments on the Tacotron2 speech synthesis system to demonstrate the effectiveness of the proposed method. The method in the study introduces an additional task: wavelet spectrograms. The continuous wavelet transform has gained significant popularity in various applications, including speech enhancement and speech recognition, which is primarily attributed to its capability to adaptively vary the time-frequency resolution and its excellent performance in capturing non-stationary signals. This study highlights that the clarity of Tacotron2 synthesized speech can be improved by introducing Wavelet-spectrogram as an auxiliary task through theoretical and experimental analysis: a feature extraction network is added, and Wavelet-spectrogram features are extracted from the Mel spectrum output generated by the decoder. Experimental findings indicate that the Mean Opinion Score achieved for the speech synthesized by the model using multi-task learning is 0.17 higher compared to the baseline model. Furthermore, by analyzing the factors contributing to the success of the continuous wavelet transform-based multi-task learning method in the Tacotron2 model, as well as the effectiveness of multi-task learning, the study conjectures that the proposed method has the potential to enhance the performance of other acoustic models.
Neighbor-based joint spatial division and multiplexing in massive MIMO: user scheduling and dynamic beam allocation
Authors: Liang Huibin , Liu Chen , Song Yunchao , Gao Tianbao , Zou Yulong
Published: January 2024

Two-stage precoding schemes have been developed to reduce the channel estimation overhead in FDD systems. By integrating user scheduling into these schemes, it becomes possible to meet the quality-of-service requirements of high-density wireless communication systems, despite the limitations on spatial resources and transmit power budget. In this paper, we propose a user scheduling and dynamic beam allocation method for neighbor-based joint spatial division multiplexing (N-JSDM) transmission. The user scheduling problem is formulated as a 0–1 quadratic programming problem to maximize effective spectral efficiency (ESE) using directional channel properties. To address the complexity issue, convex relaxation and linearization methods are employed to transform the problem into a 0–1 mixed integer linear programming, and a dimensionality reduction method is introduced. The proposed user scheduling-aided N-JSDM scheme reduces downlink training length and feedback of channel state information. Additionally, a dynamic configuration form is used for pre-beamforming matrix design based on user distribution, outperforming conventional approaches. Simulation results demonstrate higher ESE achieved by the proposed scheme compared to previous methods.
RETRACTED ARTICLE: IoT devices and data availability optimization by ANN and KNN
Authors: Chen Zhiqiang , Song Zhihua , Zhang Tao , Wei Yong
Published: January 2024

Extensive research has been conducted to enhance the availability of IoT devices and data by focusing on the rapid prediction of instantaneous fault rates and temperatures. Temperature plays a crucial role in device availability as it significantly impacts equipment performance and lifespan. It serves as a vital indicator for predicting equipment failure and enables the improvement of availability and efficiency through effective temperature management. In the proposed optimization scheme for IoT device and data availability, the artificial neural network (ANN) algorithm and the K-Nearest Neighbours (KNN) algorithm are utilized to drive a neural network. The preliminary algorithm for availability optimization is chosen, and the target is divided into two parts: data optimization and equipment optimization. Suitable models are constructed for each part, and the KNN-driven neural network algorithm is employed to solve the proposed optimization model. The effectiveness of the proposed scheme is clearly demonstrated by the verification results. When compared to the benchmark method, the availability forward fault-tolerant method, and the heuristic optimization algorithm, the maximum temperature was successfully reduced to 2.0750 °C. Moreover, significant enhancements in the average availability of IoT devices were achieved, with improvements of 27.03%, 15.76%, and 10.85% respectively compared to the aforementioned methods. The instantaneous failure rates were 100%, 87.89%, and 84.4% respectively for the three algorithms. This optimization algorithm proves highly efficient in eliminating fault signals and optimizing the prediction of time-limited satisfaction. Furthermore, it exhibits strategic foresight in the decision-making process.
Intravenous iron versus blood transfusion for postpartum anemia: a systematic review and meta-analysis
Authors: Caljé E. , Groom K. M. , Dixon L. , Marriott J. , Foon R. , Oyston C. , Bloomfield F. H. , Jordan V.
Published: January 2024

Background

Intravenous iron (IV-iron) is used as an alternative to, or alongside, red blood cell transfusion (RBC-T) to treat more severe postpartum anemia (PPA), although optimal treatment options remain unclear. No previous systematic reviews have examined IV-iron and RBC-T, including patient-reported outcomes and hematological responses.

Methods

A systematic review and meta-analysis of randomized trials comparing IV-iron and RBC-T with each other, oral iron, no treatment, and placebo for the treatment of PPA. Key inclusion criteria were PPA (hemoglobin < 12 g/dL) and IV-iron or RBC-T as interventions. Key exclusion criteria were antenatal IV-iron or RBC-T. Fatigue was the primary outcome. Secondary outcomes included hemoglobin and ferritin concentrations, and adverse events. From 27th August 2020 to 26th September 2022, databases, registries, and hand searches identified studies. A fixed-effect meta-analysis was undertaken using RevMan (5.4) software. The quality of the studies and the evidence was assessed using the Cochrane Risk of Bias table, and Grading of Recommendations, Assessment, Development, and Evaluation. This review is registered with the Prospective Register of Systematic Reviews (CRD42020201115).

Results

Twenty studies and 4196 participants were included: 1834 assigned IV-iron, 1771 assigned oral iron, 330 assigned RBC-T, and 261 assigned non-intervention. Six studies reported the primary outcome of fatigue (1251 participants). Only studies of IV-iron vs. oral iron (15 studies) were available for meta-analysis. Of these, three reported on fatigue using different scales; two were available for meta-analysis. There was a significant reduction in fatigue with IV-iron compared to oral iron (standardized mean difference − 0.40, 95% confidence interval (CI) − 0.62, − 0.18, I2 = 0%). The direction of effect also favored IV-iron for hemoglobin (mean difference (MD) 0.54 g/dL, 95% confidence interval (CI) 0.47, 0.61, I2 = 91%), ferritin, (MD 58.07 mcg/L, 95% CI 55.74, 60.41, I2 = 99%), and total adverse events (risk-ratio 0.63, 95% CI 0.52, 0.77, I2 = 84%). The overall quality of the evidence was low-moderate.

Discussion

For all outcomes, the evidence for RBC-T, compared to IV-iron, non-intervention, or dose effects of RBC-T is very limited. Further research is needed to determine whether RBC-T or IV-iron for the treatment of PPA is superior for fatigue and hematological outcomes.
Publication bias in otorhinolaryngology meta-analyses in 2021
Authors: Mohammadian Fatemeh , Bastaninejad Shahin , Irani Shirin
Published: January 2024

Introduction

One concern in meta-analyses is the presence of publication bias (PB) which leads to the dissemination of inflated results. In this study, we assessed how much the meta-analyses in the field of otorhinolaryngology in 2021 evaluated the presence of PB.

Methods

Six of the most influential journals in the field were selected. A search was conducted, and data were extracted from the included studies. In cases where PB was not assessed by the authors, we evaluated the risk of its presence by designing funnel plots and performing statistical tests.

Results

Seventy-five systematic reviews were included. Fifty-one percent of them used at least one method for assessing the risk of PB, with the visual inspection of a funnel plot being the most frequent method used. Twenty-nine percent of the studies reported a high risk of PB presence. We replicated the results of 11 meta-analyses that did not assess the risk of PB and found that 63.6% were at high risk. We also found that a considerable proportion of the systematic reviews that found a high risk of PB did not take it into consideration when making conclusions and discussing their results.

Discussion

Our results indicate that systematic reviews published in some of the most influential journals in the field do not implement enough measures in their search strategies to reduce the risk of PB, nor do they assess the risk of its presence or take the risk of its presence into consideration when inferring their results.
Respiratory function in healthy long-term meditators: a systematic review
Authors: Karunarathne L. J. U. , Amarasiri W. A. D. L. , Fernando A. D. A.
Published: January 2024

Background

There is a paucity of data on effects of long-term meditation on respiration. This systematic review summarized selected respiratory function parameters in healthy long-term meditators (LTMs) at rest, during meditation and their associations with meditation practice variables.

Methods

A systematic search of PubMed, EMBASE (Ovid), Scopus, Proquest Dissertation and Thesis Global databases, CENTRAL, and Google Scholar was performed from year 1950 to August 15th, 2023. Keywords “meditation,” “long-term meditation,” and respiratory/pulmonary/lung function and spirometry were used. Controlled-trials and observational studies exploring respiratory parameters in healthy LTMs published in English were included. Two independent reviewers selected studies, extracted data, and assessed the quality of the evidence. The Joanna-Briggs Institute Critical Appraisal Tools and the Single-Case Reporting Guideline In BEhavioural Interventions Statement were used to assess the methodological quality of the included studies. This review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Effect estimates of some outcomes were synthesized using alternative methods and data for other outcomes synthesized narratively as a meta-analysis was not possible.

Results

Nine studies comprising 3 case–control, 3 cross-sectional, and 3 single-subject study designs, involving 433 participants that met the eligibility criteria, were included. Two studies reported slower resting RR among LTMs compared to controls [SMD = − 2.98, 95% CI (− 4.48 to − 1.47), overall-effect (z-score) = 3.88 (p < 0.001), I2 = 69%] with similar trend reported in the third study (MD = − 1.6, p = 0.053). Three studies reported slower RR in LTMs during meditation compared to baseline. Slower resting RR and mean RR change during meditation compared to baseline significantly negatively associated with meditation experience. PEFR was significantly higher in LTMs than controls [MD = 1.67, 95% CI (0.19–3.15), z-score = 2.21 (p = 0.03)]. No significant difference was observed in tidal volume [SDM = 0.93, 95% CI (− 1.13 to 2.99), z-score = 0.89 (p = 0.37), I2 = 96%] and vital capacity [SDM = 1.25, 95% CI (− 0.45 to 2.95), z-score = 1.44 (p = 0.15), I2 = 94%] of LTMs compared to controls.

Conclusions

Long-term meditation appears to be associated with slower baseline RR, and immediate reduction in RR during meditation, where greater practice amplifies the effects. Evidence on spirometry parameters in LTMs with ≥ 3 years of practice was limited.