bluuebunny/crossref_metadata_2025 huggingface dataset:
Microstructural Properties and Wear Resistance of Fe-Cr-Co-Ni-Mo-Based High Entropy Alloy Coatings Deposited with Different Coating Techniques
Authors: Oppong Boakye Gifty , Geambazu Laura E. , Ormsdottir Arna M. , Gunnarsson Baldur G. , Csaki Ioana , Fanicchia Francesco , Kovalov Danyil , Karlsdottir Sigrun N.
Published: March 2022

Materials can be subjected to severe wear and corrosion due to high temperature, high pressure and mechanical loads when used in components for the production of geothermal power. In an effort to increase the lifetime of these components and thus decrease cost due to maintenance High-Entropy Alloy Coatings (HEACs) were developed with different coating techniques for anti-wear properties. The microstructure, mechanical and tribological properties of CoCrFeNiMox (at% x = 20, 27) HEACs deposited by three different technologies—high-velocity oxygen fuel (HVOF), laser cladding (LC) and electro-spark deposition (ESD)—are presented in this study. The relationship between surface morphology and microstructural properties of the as-deposited coatings and their friction and wear behavior is assessed to evaluate their candidacy as coatings for the geothermal environment. The wear rates were lower for the HVOF coatings compared to LC and ESD-produced coatings. Similarly, a higher hardness (445 ± 51 HV) was observed for the HVOF HEACs. The mixed FCC, BCC structure and the extent of σ + µ nano precipitates are considered responsible for the increased hardness and improved tribological performance of the HEACs. The findings from the study are valuable for the development of wear-resistant HEAC for geothermal energy industry applications where high wear is encountered.
Bio-Augmentation as an Emerging Strategy to Improve the Textile Compost Quality Using Identified Autochthonous Strains
Authors: Biyada Saloua , Imtara Hamada , Elkarrach Karima , Laidi Omar , Saleh Asmaa , Al Kamaly Omkulthom , Merzouki Mohammed
Published: March 2022

The present investigation is devoted, for the first time, to the potential of autochthonous inoculums through bio-augmentation tests to improve the compost quality and to decrease the composting time during composting of textile waste. For this reason, three strains were isolated from a mixture of textile waste, green waste, paper, and cardboard waste, and therefore identified as Streptomyces cellulosae, Achromobacter xylosoxidans, and Serratia liquefaciens, employed using bio-augmentation test. The organic matter decaying was assessed according to three different inoculums doses, separately and in consortium (4%, 6%, and 8%), to describe the effect of bio-augmentation process on the organic matter decaying. Indeed, these three strains and their consortium have shown a strong potential of organic matter degradation, equally the bacterial consortium showed a total organic carbon degradation of 20.3%, total Kjeldahl nitrogen of 1.52%, and a Carbon/Nitrogen ratio of 13.36. Compost maturity has been completed after only 12 weeks of treatment instead of 44 weeks using the classical treatment by composting. Ultimately, according to these results, bio-augmentation could be an emerging and promising strategy to accelerate the composting process of solid waste, especially in the case of industrial waste. Equally, it could be an effective tool to avoid the accumulation of industrial waste disposal in public landfills and/or nature while allowing their treatment.
Rice Industry By-Products as Adsorbent Materials for Removing Fluoride and Arsenic from Drinking Water—A Review
Authors: Collivignarelli Maria Cristina , Sorlini Sabrina , Milanese Chiara , Illankoon W. A. M. A. N. , Caccamo Francesca Maria , Calatroni Silvia
Published: March 2022

In drinking water, high concentrations of fluoride and arsenic can have adverse effects on human health. Waste deriving from the rice industry (rice husk, rice straw, rice bran) can be promising adsorbent materials, because they are (i) produced in large quantities in many parts of the world, (ii) recoverable in a circular economy perspective, (iii) at low cost if compared to expensive conventional activated carbon, and (iv) easily manageable even in developing countries. For the removal of fluoride, rice husk and rice straw allowed to obtain adsorption capacities in the range of 7.9–15.2 mg/g. Using rice husk for arsenic adsorption, excellent results were achieved with adsorption capacities above 19 mg/g. The best results both for fluorides and arsenic (>50 mg/g) were found with metal- or chemical-modified rice straw and rice husk. Identifying the next steps of future research to ensure the upscaling of biochar from recovered by-products, it is fundamental to perform: (i) tests on real waters for multicomponent adsorption; (ii) experiments with pilot plants in continuous operation; (iii) cost analysis/real applicability of modification treatments such as metal coupling or chemical synthesis; (iv) more studies on the biochar stability and on its regeneration or recovery after use.
Explorative Supercooling Technology for Prevention of Freeze Damages in Vaccines
Authors: Jun Shawn , Ko Youngbok , Lee Seung Hyun
Published: March 2022

Most freeze-sensitive vaccines are stored between 2 °C and 8 °C upon manufacturing and until they are eventually administered in intermediate vaccine stores and health facilities. This so-called “cold chain” of vaccine distribution is strictly regulated at these specific temperatures to avoid freeze damage. Liquid formulations of particular vaccines (e.g., aluminum-adsorbed tetanus toxoid (TT)) will irreversibly lose their immunogenicity once frozen. Using an oscillating magnetic field (OMF), supercooling can inhibit ice crystal nucleation effectively; water is susceptible to influence by a strong magnetic field, allowing normal water dynamics even in subzero freezing conditions. This recently developed technology—composed of a custom-designed electromagnet unit producing an optimal field strength (50 mT) at a specific frequency (1 Hz)—was successfully used to inhibit the formation of ice crystals in aluminum adjuvant TT vaccines, therefore preventing any visible damage in the vaccines’ microscopic structure. Despite being subject to temperatures far below their freezing point (up to −14 °C) for up to seven days, the TT vaccines showed no freeze damage on physical appearances. Results were further validated using shake tests and light microscopy. As storage and freeze-protection become more critical during times of increased vaccination efforts—particularly against COVID-19—this supercooling technology can be a promising solution to distribution problems by removing concern for temperature abuse or shock-induced freezing.
Inkjet-Printed Flexible Strain-Gauge Sensor on Polymer Substrate: Topographical Analysis of Sensitivity
Authors: Kang Hyunkyoo , Kim Seokjin , Shin Jaehak , Ko Sunglim
Published: March 2022

Inkjet-printed strain gauges on flexible substrates have recently been investigated for biomedical motion detection as well as the monitoring of structural deformation. This study performed a topographical analysis of an inkjet-printed strain gauge constructed using silver conductive ink on a PET (polyethylene terephthalate) substrate. Serpentine strain-gauge sensors of various thicknesses and widths were fabricated using inkjet printing and oven sintering. The fabricated gauge sensors were attached to curved surfaces, and gauge factors ranging from 2.047 to 3.098 were recorded. We found that the cross-sectional area of the printed strain gauge was proportional to the gauge factor. The correlation was mathematically modelled as y = 0.4167ln(x) + 1.3837, for which the coefficient of determination (R2) was 0.8383.
Application of Nanofluids in CO2 Absorption: A Review
Authors: Aghel Babak , Janati Sara , Alobaid Falah , Almoslh Adel , Epple Bernd
Published: March 2022

The continuous release of CO2 into the atmosphere as a major cause of increasing global warming has become a growing concern for the environment. Accordingly, CO2 absorption through an approach with maximum absorption efficiency and minimum energy consumption is of paramount importance. Thanks to the emergence of nanotechnology and its unique advantages in various fields, a new approach was introduced using suspended particles in a base liquid (suspension) to increase CO2 absorption. This review article addresses the performance of nanofluids, preparation methods, and their stability, which is one of the essential factors preventing sedimentation of nanofluids. This article aims to comprehensibly study the factors contributing to CO2 absorption through nanofluids, which mainly addresses the role of the base liquids and the reason behind their selection.
Further Advances in Atrial Fibrillation Research: A Metabolomic Perspective
Authors: Arbeloa-Gómez Laura , Álvarez-Vidal Jaime , Izquierdo-García Jose Luis
Published: March 2022

Atrial fibrillation involves an important type of heart arrhythmia caused by a lack of control in the electrical signals that arrive in the heart, produce an irregular auricular contraction, and induce blood clotting, which finally can lead to stroke. Atrial fibrillation presents some specific characteristics, but it has been treated and prevented using conventional methods similar to those applied to other cardiovascular diseases. However, due to the influence of this pathology on the mortality caused by cerebrovascular accidents, further studies on the molecular mechanism of atrial fibrillation are required. Our aim here is provide a compressive review of the use of metabolomics on this condition, from the study of the metabolic profile of plasma to the development of animal models. In summary, most of the reported studies highlighted alterations in the energetic pathways related to the development of the condition.
An Environmentally Friendly Technology of Metal Fiber Bag Filter to Purify Dust-Laden Airflow
Authors: Zhang Mingxing , Qin Wenqian , Ma Xiaohui , Liu Anxiong , Yan Cuiping , Li Peng , Huang Mei , He Chunhong
Published: March 2022

Over the past decades, China has suffered from negative environmental impacts from distempered dust-laden airflow-purification activities. After a decade of effort, dust-laden airflow purification and powder particle recycling have been realized in specialized companies in China, and law enforcement for illegal activities of dust-laden airflow discharge has also been made increasingly strict. Thus, up to now, dust-laden airflow purification in China should be developed toward being more in-depth and refined to promote industrial applications of dust-laden airflow purification. This article reviews the status of existing technologies for dust-laden airflow purification. A novel and environmentally friendly technology for purifying the dust-laden airflow is proposed which uses a metal bag filter to collect dust particles. The bottlenecks in the dust-laden airflow-purification system are analyzed. Some preliminary experiments of pinch technologies are also conducted. Finally, in order to provide directional guidance for the future development of metal bag filters, some key points regarding the metal bag filter purification system are proposed to point towards a future trend in dust-laden airflow purification.
Particulate Matter in Swine Barns: A Comprehensive Review
Authors: Yang Xufei , Haleem Noor , Osabutey Augustina , Cen Zhisheng , Albert Karlee L. , Autenrieth Daniel
Published: March 2022

Particulate matter (PM) represents an air quality management challenge for confined swine production systems. Due to the limited space and ventilation rate, PM can reach relatively high concentrations in swine barns. PM in swine barns possesses different physical, chemical, and biological characteristics than that in the atmosphere and other indoor environments. As a result, it exerts different environmental and health effects and creates some unique challenges regarding PM measurement and mitigation. Numerous research efforts have been made, generating massive data and information. However, relevant review reports are sporadic. This study aims to provide an updated comprehensive review of swine barn PM, focusing on publications since 1990. It covers various topics including PM characteristics, sources, measurement methods, and in-barn mitigation technologies. As PM in swine barns is primarily of biological origins, bioaerosols are reviewed in great detail. Relevant topics include bacterial/fungal counts, viruses, microbial community composition, antibiotic-resistant bacteria, antibiotic resistance genes, endotoxins, and (1→3)-β-D-glucans. For each topic, existing knowledge is summarized and discussed and knowledge gaps are identified. Overall, PM in swine barns is complicated in chemical and biological composition and highly variable in mass concentrations, size, and microbial abundance. Feed, feces, and skins constitute the major PM sources. Regarding in-barn PM mitigation, four technologies (oil/water sprinkling, ionization, alternation of feed and feeders, and recirculating air filtration) are dominant. However, none of them have been widely used in commercial barns. A collective discussion of major knowledge gaps and future research needs is offered at the end of the report.
Comparison between Air Temperature and Land Surface Temperature for the City of São Paulo, Brazil
Authors: do Nascimento Augusto Cezar Lima , Galvani Emerson , Gobo João Paulo Assis , Wollmann Cássio Arthur
Published: March 2022

This study aims to identify the relationship between changes in temperature regarding urbanization processes and seasonality in the city of São Paulo, located in the Tropic of Capricorn. The land surface temperature (LST) results were compared to official weather stations measurements, identifying in the spring–summer period 65.5% to 86.2% accuracy, while in the autumn–winter period, the results ranged from 58.6% to 93.1% accuracy, when considering the standard deviation and the temperature probe error. The mean MAE and mean RMSE range from 1.2 to 1.9 °C, with 83.0% of the values being ≤2.7 °C, and the coefficient of determination values are R = 0.81 in spring–summer and R = 0.82 in autumn–winter. Great thermal amplitude was estimated in the spring–summer season, with a difference in LST of the built-up space and rural area ranging from 5.8 and 11.5 °C, while in the autumn–winter season, the LST is more distributed through the city, with differences ranging from 4.4 to 8.5 °C. In addition, the current study suggests remote sensing as a reliable, cheap, and practical methodology to assist climate in order to support public policies and decision-making actions regarding environmental and urban planning.
Adopting a Whole Systems Approach to Transport Decarbonisation, Air Quality and Health: An Online Participatory Systems Mapping Case Study in the UK
Authors: Penn Alexandra S. , Bartington Suzanne E. , Moller Sarah J. , Hamilton Ian , Levine James G. , Hatcher Kirstie , Gilbert Nigel
Published: March 2022

In a drive to achieve net zero emissions, U.K. transport decarbonisation policies are predominantly focussed on measures to promote the uptake and use of electric vehicles (EVs). This is reflected in the COP26 Transport Declaration signed by 38 national governments, alongside city region governments, vehicle manufacturers and investors. However, emerging evidence suggests that EVs present multiple challenges for air quality, mobility and health, including risks from non-exhaust emissions (NEEs) and increasing reliance on vehicles for short trips. Understanding the interconnected links between electric mobility, human health and the environment, including synergies and trade-offs, requires a whole systems approach to transport policymaking. In the present paper, we describe the use of Participatory Systems Mapping (PSM) in which a diverse group of stakeholders collaboratively constructed a causal model of the U.K. surface transport system through a series of interactive online workshops. We present the map and its analysis, with our findings illustrating how unintended consequences of EV-focussed transport policies may have an impact on air quality, human health and important social functions of the transport system. We conclude by considering how online participatory causal modelling techniques could be effectively integrated with empirical metrics to facilitate effective policy design and appraisal in the transport sector.
Service Oriented R-ANN Knowledge Model for Social Internet of Things
Authors: S. D. Mohana , Prakash S. P. Shiva , Krinkin Kirill
Published: March 2022

Increase in technologies around the world requires adding intelligence to the objects, and making it a smart object in an environment leads to the Social Internet of Things (SIoT). These social objects are uniquely identifiable, transferable and share information from user-to-objects and objects-to objects through interactions in a smart environment such as smart homes, smart cities and many more applications. SIoT faces certain challenges such as handling of heterogeneous objects, selection of generated data in objects, missing values in data. Therefore, the discovery and communication of meaningful patterns in data are more important for every application. Thus, the analysis of data is essential in smarter decisions and qualifies performance of data for various applications. In a smart environment, social networks of intelligent objects are increasing services and decreasing the relationship in a reliable and efficient way of sharing resources and services. Hence, this work proposed the feature selection method based on proposed semantic rules and established the relationships to classify the services using relationship artificial neural networks (R-ANN). R-ANN is an inversely proportional relationship to the objects based on certain rules and conditions between the objects to objects and users to objects. It provides the service oriented knowledge model to make decisions in the proposed R-ANN model that produces service to the users. The proposed R-ANN provides an accuracy of 89.62% for various services namely weather, air quality, parking, light status, and people presence respectively in the SIoT environment compared to the existing model.
Effects of the Leptin-Mediated MAPK/ERK Signaling Pathway on Collagen II Expression in Knee Cartilage of Newborn Male Mice from Obese Maternal Offspring
Authors: Wang Wenji , Zhang Jialing , Huo Yu , Zheng Yuanzheng , Gui Yonghao
Published: March 2022

Epidemiological data suggest that various noncommunicable diseases develop as a result of altered maternal metabolic and physiological status due to exposure to several adverse factors during pregnancy. However, evidence for intrauterine exposure factors and mechanisms underlying the origin of early cartilage disease in chronic osteoarthritic disease is still lacking. In this study, we found that persistent overnutrition during pregnancy in obese mothers led to cartilage damage in neonatal male mice. This was mainly characterized by increased apoptosis with decreased expression of chondrocyte collagen II and low expression of Runx family transcription factor 2 (RUNX2) and SRY-box transcription factor 9 (SOX9). This reduction was also found to be associated with high leptin expression in newborn male mice of obese maternal offspring. Furthermore, the administration of leptin and mitogen-activated protein kinase (MAPK)-extracellular signal-regulated kinase (ERK) inhibitors in primary chondrocytes showed that leptin mediated MAPK/ERK signaling activation and thus affected the key regulators of cartilage matrix metallopeptidase 1 (MMP1) and tissue inhibitor of metalloproteinase 1 (TIMP1), thereby altering the expression of collagen II in mouse cartilage. Altogether, this study provided insights into the molecular mechanisms of cartilage-related disease development and also new clues and evidence for the fetogenetic origin of cartilage diseases.
Preparation and Applications of Electrospun Nanofibers for Wearable Biosensors
Authors: Xu Tengzhou , Ji Guojing , Li Hui , Li Jiaduo , Chen Zhou , Awuye Desire Emefa , Huang Jie
Published: March 2022

The emergence of nanotechnology has provided many new ideas and innovations in the field of biosensors. Electrospun nanofibers have many excellent properties such as high specific surface area, high porosity, low cost, high efficiency, and they can be combined with a variety of sensors. These remarkable features have a wide range of applications in the field of sensors such as monitoring air pollutants, highly sensitive pressure sensors, and biosensors for monitoring the pulse of the body. This paper summarizes the working principle and influencing factors of electrospinning nanofibers, and illustrates their applications in wearable biosensors.
DNA-Based Biosensors for the Biochemical Analysis: A Review
Authors: Hua Yu , Ma Jiaming , Li Dachao , Wang Ridong
Published: March 2022

In recent years, DNA-based biosensors have shown great potential as the candidate of the next generation biomedical detection device due to their robust chemical properties and customizable biosensing functions. Compared with the conventional biosensors, the DNA-based biosensors have advantages such as wider detection targets, more durable lifetime, and lower production cost. Additionally, the ingenious DNA structures can control the signal conduction near the biosensor surface, which could significantly improve the performance of biosensors. In order to show a big picture of the DNA biosensor’s advantages, this article reviews the background knowledge and recent advances of DNA-based biosensors, including the functional DNA strands-based biosensors, DNA hybridization-based biosensors, and DNA templated biosensors. Then, the challenges and future directions of DNA-based biosensors are discussed and proposed.
Folic Acid-Modified Fluorescent-Magnetic Nanoparticles for Efficient Isolation and Identification of Circulating Tumor Cells in Ovarian Cancer
Authors: Pan Yue , Wang Zhili , Ma Jialing , Zhou Tongping , Wu Zeen , Ding Pi , Sun Na , Liu Lifen , Pei Renjun , Zhu Weipei
Published: March 2022

Ovarian cancer (OC) is a lethal disease occurring in women worldwide. Due to the lack of obvious clinical symptoms and sensitivity biomarkers, OC patients are often diagnosed in advanced stages and suffer a poor prognosis. Circulating tumor cells (CTCs), released from tumor sites into the peripheral blood, have been recognized as promising biomarkers in cancer prognosis, treatment monitoring, and metastasis diagnosis. However, the number of CTCs in peripheral blood is low, and it is a technical challenge to isolate, enrich, and identify CTCs from the blood samples of patients. This work develops a simple, effective, and inexpensive strategy to capture and identify CTCs from OC blood samples using the folic acid (FA) and antifouling-hydrogel-modified fluorescent-magnetic nanoparticles. The hydrogel showed a good antifouling property against peripheral blood mononuclear cells (PBMCs). The FA was coupled to the hydrogel surface as the targeting molecule for the CTC isolation, held a good capture efficiency for SK-OV-3 cells (95.58%), and successfully isolated 2–12 CTCs from 10 OC patients’ blood samples. The FA-modified fluorescent-magnetic nanoparticles were successfully used for the capture and direct identification of CTCs from the blood samples of OC patients.
Distinct Effects of Stimulus Repetition on Various Temporal Stages of Subject’s Own Name Processing
Authors: Zhang Yihui , Xie Musi , Wang Yuzhi , Qin Pengmin
Published: March 2022

The self is one of the most important concepts in psychology, which is of great significance for human survival and development. As an important self-related stimulus, the subject’s own name (SON) shows great advantages in cognitive and social processing and is widely used as an oddball stimulus in previous studies. However, it remained unknown whether the multiple repetition of stimulus would have similar influence on the neural response to SON and the other names under equal probability. In this study, adopting EEG and an equal–probability paradigm, we first detected the SON-related ERP components which could differentiate SON from other names, and then investigated how these components are influenced by repeated exposure of the stimulus. Our results showed that SON evoked an earlier SON-related negativity (SRN) at the fronto-central region and a late positive potential (LPP) at the centro-parietal region. More intriguingly, the earlier SRN demonstrated reduction after multiple repetitions, whereas LPP did not exhibit significant changes. In conclusion, these findings revealed that multiple repetitions of the stimulus might influence the various temporal stages in SON-related processing and highlighted the robustness of the late stage in this processing.
Later but Not Weaker: Neural Categorization of Native Vowels of Children at Familial Risk of Dyslexia
Authors: Chen Ao
Published: March 2022

Although allophonic speech processing has been hypothesized to be a contributing factor in developmental dyslexia, experimental evidence is limited and inconsistent. The current study compared the categorization of native similar sounding vowels of typically developing (TD) children and children at familial risk (FR) of dyslexia. EEG response was collected in a non-attentive passive oddball paradigm from 35 TD and 35 FR Dutch 20-month-old infants who were matched on vocabulary. The children were presented with two nonwords “giep” [ɣip] and “gip” [ɣIp] that contrasted solely with respect to the vowel. In the multiple-speaker condition, both nonwords were produced by twelve different speakers while in the single-speaker condition, single tokens of each word were used as stimuli. For both conditions and for both groups, infant positive mismatch response (p-MMR) was elicited, and the p-MMR amplitude was comparable between the two groups, although the FR children had a later p-MMR peak than the TD children in the multiple-speaker condition. These findings indicate that FR children are able to categorize speech sounds, but that they may do so in a more effortful way than TDs.
Experimental Research on the Mechanical Properties and Autogenous Shrinkage of Precast Members Joint Concrete
Authors: Zhu Haitang , He Yunjian , Ji Dongdong , Tang Jiyu , Li Chuanchuan
Published: March 2022

In the precast building structure, the joint is the key part that affects the mechanical performance of the assembly structure. By selecting coarse and fine fraction aggregate; adding high quality mineral admixture, expansive agent, and water-reducing agent; and optimizing the mixture ratio design, precast members joint concrete (PMJC) with self-compacting performance, micro-shrinkage, good volume stability, and strength matching with precast concrete is developed. In this study, the PMJC with a water-binder ratio, fly ash, ultra-fine silicon powder, sand rate, expansive agent, and water-reducing agent of 0.38, 30%, 3%, 47.7%, 8%, and 0.7%, respectively, showed a good workability which could meet the conditions of on-site construction. In addition, the strength matched the precast members, and the PMJC had certain ductility and stability, small early contraction, and met the requirements of the joint material.
Finite Element Analysis of Frames with Reinforced Concrete Encased Steel Composite Columns
Authors: Tunc Gokhan , Othman Mohammed Moatasem , Mertol Halit Cenan
Published: March 2022

Structural frame systems that consists of concrete-encased-steel-embedded composite columns and reinforced concrete beams are typically used in mid-rise to tall buildings. In order to understand their overall structural behavior, a total of 12 frame models with high and low ductility features were constructed and analyzed using LS-DYNA software. Two of these models were validated using the results of previously tested frames. The remaining 10 models were studied to predict the behavior of frames with varying concrete strengths, reinforcement configurations, and structural steel sections under vertical and lateral loads. The results were investigated in terms of cracks and failure patterns, load-deflection relationships, energy dissipation, and stiffness degradation. The analytical results indicated that the high ductile frame models showed slightly better lateral load carrying performances compared to low ductility frame models. Moreover, the analytical studies demonstrated that the existence of structural steel in a column, regardless of its cross-sectional shape, was the most important parameter in improving the lateral load carrying capacity of a frame.
Determination of Optimum Building Envelope Parameters of a Room concerning Window-to-Wall Ratio, Orientation, Insulation Thickness and Window Type
Authors: Altun Ayşe Fidan
Published: March 2022

The building envelope includes all materials (glazing, external walls, doors, etc.) that separate the conditioned space from the outside environment. Building envelope characteristics significantly influence the energy consumption of buildings. In this study, research was carried out to find optimum building envelope design parameters, such as insulation thickness, orientation, glazing type, and the window-to-wall ratio of a room, using actual climatic data of two cities with different characteristics according to the Köppen climatic classification. The insulation thickness and the window type that minimizes the net present worth of the building façade over 20 years of a lifetime gave the optimum values. In addition, the effect of the various parameters, such as the infiltration rate through the envelope, room set-point temperature, and the fuel type, on the net present cost was also analyzed. It was found that appropriate selection of windows, orientation, and insulation thickness would lead to a significant reduction in the annual energy consumption. Despite having the lowest initial investment cost, the room with single glazed windows had the highest energy requirement and the net present cost. The building façade with double glazed windows, oriented towards the south-west, yielded the minimum net present cost in both locations. Results showed that the optimum external wall thickness is 9 cm in Hakkari (Dsa—Continental Climate) and 6 cm in Istanbul (Csa—Mild Climate).
Low-Temperature SCR Catalyst Development and Industrial Applications in China
Authors: Zhu Hongtai , Song Liyun , Li Kai , Wu Rui , Qiu Wenge , He Hong
Published: March 2022

In recent years, low-temperature SCR (Selective Catalytic Reduction) denitrification technology has been popularized in non-power industries and has played an important role in the control of industrial flue gas NOx emissions in China. Currently, the most commonly used catalysts in industry are V2O5-WO3(MoO3)/TiO2, MnO2-based catalysts, CeO2-based catalysts, MnO2-CeO2 catalysts and zeolite SCR catalysts. The flue gas emitted during industrial combustion usually contains SO2, moisture and alkali metals, which can affect the service life of SCR catalysts. This paper summarizes the mechanism of catalyst poisoning and aims to reduce the negative effect of NH4HSO4 on the activity of the SCR catalyst at low temperatures in industrial applications. It also presents the outstanding achievements of domestic companies in denitrification in the non-power industry in recent years. Much progress has been made in the research and application of low-temperature NH3-SCR, and with the renewed demand for deeper NOx treatments, new technologies with lower energy consumption and more functions need to be developed.
Effect of Plating Variables on Oxygen Evolution Reaction of Ni–Zn–Fe Electrodes for Alkaline Water Electrolysis
Authors: Lee Su-han , Kim Ji Eun , Joo Hyunku , Park Chu-sik , Jeong Seong-uk , Jung Kwangjin , Kim Young-ho , Kang Kyoung-soo
Published: March 2022

In this study, we investigated the oxygen evolution reaction (OER) characteristics of Ni–Zn–Fe electrodes by varying plating current density and Ni:Fe ratio in a plating bath. The activity of the OER increased up to the plating current density of 160 mA/cm2, as the Fe content of the deposited electrode increased and electrochemical surface area (ECSA) increased after Zn dealloying. However, for the plated electrode with higher than 160 mA/cm2 of current density, the change in composition caused by underpotential deposition led to decreased activity due to decreasing Fe content and diminishing Zn dealloying. Moreover, when the Ni:Fe ratio in the plating bath was varied, outstanding OER activity was observed at Ni:Fe = 2:1. When the Fe content of the bath increased beyond this ratio, Fe could not restrain Ni oxidation and formed Fe oxides in OER reaction, and oxygen vacancy decreased. These caused a degradation of the OER activity.
Clinical Trials Using Mesenchymal Stem Cells for Spinal Cord Injury: Challenges in Generating Evidence
Authors: de Araújo Lila Teixeira , Macêdo Carolina Thé , Damasceno Patrícia Kauanna Fonseca , das Neves Ítalo Gabriel Costa , de Lima Carla Souza , Santos Girlaine Café , de Santana Thaís Alves , Sampaio Gabriela Louise de Almeida , Silva Daniela Nascimento , Villarreal Cristiane Flora , Chaguri Alessandra Casemiro de Campos , da Silva Crislaine Gomes , Mota Augusto César de Andrade , Badaró Roberto , Ribeiro dos Santos Ricardo , Soares Milena Botelho Pereira
Published: March 2022

Spinal cord injury (SCI) remains an important public health problem which often causes permanent loss of muscle strength, sensation, and function below the site of the injury, generating physical, psychological, and social impacts throughout the lives of the affected individuals, since there are no effective treatments available. The use of stem cells has been investigated as a therapeutic approach for the treatment of SCI. Although a significant number of studies have been conducted in pre-clinical and clinical settings, so far there is no established cell therapy for the treatment of SCI. One aspect that makes it difficult to evaluate the efficacy is the heterogeneity of experimental designs in the clinical trials that have been published. Cell transplantation methods vary widely among the trials, and there are still no standardized protocols or recommendations for the therapeutic use of stem cells in SCI. Among the different cell types, mesenchymal stem/stromal cells (MSCs) are the most frequently tested in clinical trials for SCI treatment. This study reviews the clinical applications of MSCs for SCI, focusing on the critical analysis of 17 clinical trials published thus far, with emphasis on their design and quality. Moreover, it highlights the need for more evidence-based studies designed as randomized controlled trials and potential challenges to be addressed in context of stem cell therapies for SCI.
Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice
Authors: Tomesz Andras , Szabo Laszlo , Molnar Richard , Deutsch Arpad , Darago Richard , Raposa Bence L. , Ghodratollah Nowrasteh , Varjas Timea , Nemeth Balazs , Orsos Zsuzsanna , Pozsgai Eva , Szentpeteri Jozsef L. , Budan Ferenc , Kiss Istvan
Published: March 2022

Specific gene and miRNA expression patterns are potential early biomarkers of harmful environmental carcinogen exposures. The aim of our research was to develop an assay panel by using several miRNAs for the rapid screening of potential carcinogens. The expression changes of miR-124-1, miR-212, miR-132, miR-134, and miR-155 were examined in the spleen, liver, and kidneys of CBA/Ca mice, following the 20 mg/bwkg intraperitoneal 7,12-dimethylbenz(a)anthracene (DMBA) treatment. After 24 h RNA was isolated, the miRNA expressions were analyzed by a real-time polymerase chain reaction and compared to a non-treated control. DMBA induced significant changes in the expression of miR-134, miR-132, and miR-124-1 in all examined organs in female mice. Thus, miR-134, miR-132, and miR-124-1 were found to be suitable biomarkers for the rapid screening of potential chemical carcinogens and presumably to monitor the protective effects of chemopreventive agents.
A Novel Bioreactor for Reconstitution of the Epithelium and Submucosal Glands in Decellularized Ferret Tracheas
Authors: Pai Albert C. , Lynch Thomas J. , Ahlers Bethany A. , Ievlev Vitaly , Engelhardt John F. , Parekh Kalpaj R.
Published: March 2022

Tracheal grafts introduce the possibility to treat airway pathologies that require resection. While there has been success with engraftment of the surface airway epithelium (SAE) onto decellularized tracheas, there has been minimal advancement in regenerating the submucosal glands (SMGs). We designed a cost-effective open-system perfusion bioreactor to investigate the engraftment potential of ferret SAEs and murine myoepithelial cells (MECs) on a partly decellularized ferret trachea with the goal of creating a fully functional tracheal replacement. An air–liquid interface was also arranged by perfusing humidified air through the lumen of a recellularized conduit to induce differentiation. Our versatile bioreactor design was shown to support the successful partial decellularization and recellularization of ferret tracheas. The decellularized grafts maintained biomechanical integrity and chondrocyte viability, consistent with other publications. The scaffolds supported SAE basal cell engraftment, and early differentiation was observed once an air–liquid interface had been established. Lastly, MEC engraftment was sustained, with evidence of diffuse SMG reconstitution. This model will help shed light on SMG regeneration and basal cell differentiation in vitro for the development of fully functional tracheal grafts before transplantation.
Cerebrospinal Fluid Proteome Alterations Associated with Neuropsychiatric Symptoms in Cognitive Decline and Alzheimer’s Disease
Authors: Mroczek Magdalena , Clark Christopher , Dayon Loïc , Bowman Gene L. , Popp Julius
Published: March 2022

Although neuropsychiatric symptoms (NPS) are common and severely affect older people with cognitive decline, little is known about their underlying molecular mechanisms and relationships with Alzheimer’s disease (AD). The aim of this study was to identify and characterize cerebrospinal fluid (CSF) proteome alterations related to NPS. In a longitudinally followed-up cohort of subjects with normal cognition and patients with cognitive impairment (MCI and mild dementia) from a memory clinic setting, we quantified a panel of 790 proteins in CSF using an untargeted shotgun proteomic workflow. Regression models and pathway enrichment analysis were used to investigate protein alterations related to NPS, and to explore relationships with AD pathology and cognitive decline at follow-up visits. Regression analysis selected 27 CSF proteins associated with NPS. These associations were independent of the presence of cerebral AD pathology (defined as CSF p-tau181/Aβ1–42 > 0.0779, center cutoff). Gene ontology enrichment showed abundance alterations of proteins related to cell adhesion, immune response, and lipid metabolism, among others, in relation to NPS. Out of the selected proteins, three were associated with accelerated cognitive decline at follow-up visits after controlling for possible confounders. Specific CSF proteome alterations underlying NPS may both represent pathophysiological processes independent from AD and accelerate clinical disease progression.
Predation Stress Causes Excessive Aggression in Female Mice with Partial Genetic Inactivation of Tryptophan Hydroxylase-2: Evidence for Altered Myelination-Related Processes
Authors: Svirin Evgeniy , Veniaminova Ekaterina , Costa-Nunes João Pedro , Gorlova Anna , Umriukhin Aleksei , Kalueff Allan V. , Proshin Andrey , Anthony Daniel C. , Nedorubov Andrey , Tse Anna Chung Kwan , Walitza Susanne , Lim Lee Wei , Lesch Klaus-Peter , Strekalova Tatyana
Published: March 2022

The interaction between brain serotonin (5-HT) deficiency and environmental adversity may predispose females to excessive aggression. Specifically, complete inactivation of the gene encoding tryptophan hydroxylase-2 (Tph2) results in the absence of neuronal 5-HT synthesis and excessive aggressiveness in both male and female null mutant (Tph2−/−) mice. In heterozygous male mice (Tph2+/−), there is a moderate reduction in brain 5-HT levels, and when they are exposed to stress, they exhibit increased aggression. Here, we exposed female Tph2+/− mice to a five-day rat predation stress paradigm and assessed their emotionality and social interaction/aggression-like behaviors. Tph2+/− females exhibited excessive aggression and increased dominant behavior. Stressed mutants displayed altered gene expression of the 5-HT receptors Htr1a and Htr2a, glycogen synthase kinase-3 β (GSK-3β), and c-fos as well as myelination-related transcripts in the prefrontal cortex: myelin basic protein (Mbp), proteolipid protein 1 (Plp1), myelin-associated glycoprotein (Mag), and myelin oligodendrocyte glycoprotein (Mog). The expression of the plasticity markers synaptophysin (Syp) and cAMP response element binding protein (Creb), but not AMPA receptor subunit A2 (GluA2), were affected by genotype. Moreover, in a separate experiment, naïve female Tph2+/− mice showed signs of enhanced stress resilience in the modified swim test with repeated swimming sessions. Taken together, the combination of a moderate reduction in brain 5-HT with environmental challenges results in behavioral changes in female mice that resemble the aggression-related behavior and resilience seen in stressed male mutants; additionally, the combination is comparable to the phenotype of null mutants lacking neuronal 5-HT. Changes in myelination-associated processes are suspected to underpin the molecular mechanisms leading to aggressive behavior.
Transposable Elements: Major Players in Shaping Genomic and Evolutionary Patterns
Authors: Colonna Romano Nunzia , Fanti Laura
Published: March 2022

Transposable elements (TEs) are ubiquitous genetic elements, able to jump from one location of the genome to another, in all organisms. For this reason, on the one hand, TEs can induce deleterious mutations, causing dysfunction, disease and even lethality in individuals. On the other hand, TEs can increase genetic variability, making populations better equipped to respond adaptively to environmental change. To counteract the deleterious effects of TEs, organisms have evolved strategies to avoid their activation. However, their mobilization does occur. Usually, TEs are maintained silent through several mechanisms, but they can be reactivated during certain developmental windows. Moreover, TEs can become de-repressed because of drastic changes in the external environment. Here, we describe the ‘double life’ of TEs, being both ‘parasites’ and ‘symbionts’ of the genome. We also argue that the transposition of TEs contributes to two important evolutionary processes: the temporal dynamic of evolution and the induction of genetic variability. Finally, we discuss how the interplay between two TE-dependent phenomena, insertional mutagenesis and epigenetic plasticity, plays a role in the process of evolution.
NAADP Signaling: New Kids on the Block
Authors: Guse Andreas H.
Published: March 2022

Nicotinic acid adenine dinucleotide phosphate (NAADP) is a universal Ca2+ mobilizing second messenger essential for initiation of Ca2+ signaling. Recently, novel molecular mechanisms of both its rapid formation upon receptor stimulation and its mode of action were discovered. Dual NADPH oxidase 2 (DUOX2) and hematological and neurological expressed 1-like protein (HN1L)/Jupiter microtubule-associated homolog 2 (JPT2) were discovered as NAADP-forming enzyme and NAADP receptor/binding protein—the new kids on the block. These novel aspects are reviewed and integrated into the previous view of NAADP signaling.
The Effect of Different Surface Conditioning Techniques on the Bonding between Resin Cement & Ceramic
Authors: Gupta Shekhar , Gupta Bharti , Motwani Bhagwandas K. , Binalrimal Sultan , Radwan Waseem , Robaian Ali , Zidane Bassam , Al Wadei Mohammed Hussain Dafer , Veeraraghavan Vishnu Priya , Bhandi Shilpa , Raj A. Thirumal , Patil Shankargouda
Published: March 2022

Surface etching before cementation is a vital step that determines the clinical performance of ceramic restorations. Etching alters surface topography that contributes effective bonding between ceramic restoration and resin cement. This study aimed to compare etching techniques to determine the most effective etching method contributing the highest bond strength that helps in improving dental implants. Materials and methods: sixty discs of feldspathic ceramic measuring 10 mm diameter and 4 mm thickness were prepared. The 60 samples were divided into four equal groups based on the surface treatment technique used: group A: 9.6% hydrofluoric acid; group B: coarse diamond burs; group C: CO2 laser; and group D: no treatment. Ceramic disc specimens were examined under a Scanning Electron microscope (SEM) after surface treatment to characterize their surface morphology. Further, the specimens were luted with a resin luting agent and incubated for 24 h at a temperature of 37 °C simulating the oral environment. After 24 h, shear bond strength (SBS) and the nature of bond failure was determined for each specimen using a universal Instron testing machine. Results: significant change in surface morphology was noticed on hydrofluoric acid treatment forming larger irregular roughness (4.83 ± 1.78 µm) with multiple patterns of grooves and pores compared to other groups. Further, the highest SBS value was measured on hydrofluoric acid etching that display the highest bond strength due to the high surface roughness. In conclusion, our findings report a strong association between the surface roughness and bond strength upon hydrofluoric acid compared to other methods. Further work in this direction will enhance the utility of the etching technique on the improvement of dental implants.
Molecular Dynamics Study of the Diffusion between Virgin and Aged Asphalt Binder
Authors: Zhan Yiqun , Wu Hao , Song Weimin , Zhu Lin
Published: March 2022

The diffusion between the virgin and aged asphalt binder in the recycled asphalt mixture is a crucial factor affecting its macro-mechanical performance. In this study, a combined model of the virgin-aged layered asphalt structure was assembled based on the molecular dynamics (MD) method. A four-component and twelve-category molecule were used to model the asphalt. The diffusion behaviors of the virgin and aged asphalt were characterized by mean square displacement (MSD), diffusion coefficient, relative concentration and cohesive energy density (CED). Results indicated that at the same temperature, the diffusion coefficient of the virgin asphalt was the largest, followed by the virgin-aged asphalt and the aged asphalt. As the temperature increased, the relative concentration on both sides of the virgin-aged asphalt overlapped to a certain extent. The covered lengths of the virgin asphalt were larger than those of the aged asphalt, indicating the diffusion between the virgin asphalt and aged asphalt was mainly manifested as the diffusion from the virgin asphalt to the aged asphalt. The development of CED and the fraction of free volume (FFV) indicated the mutual attractive interactions among the molecules in virgin and aged asphalt layers became strong and the cohesion properties inside the model became better.