bluuebunny/crossref_metadata_2025 huggingface dataset:
Ethane groups modified DNA nanopores to prolong the dwell time on live cell membranes for transmembrane transport
Authors: Li Yuan , Chen Xiaolei , Lv Cheng , Cheng Yu
Published: March 2023

Transmembrane transport, mostly relying on biological channels, is crucial for the metabolic processes of live cells including sensing, signaling, cellular communicating and molecular transport. Artificial biomimetic channels offer excellent opportunities for studying the mechanisms of the metabolic processes of live cells and promote the applications of gene transfection, drug delivery, and regulations of cellular communications. DNA nanopores can be designed flexibly and operated easily while maintaining good biocompatibility, offering a good candidate for applications in basic research. However, because of the small size and good biocompatibility of DNA nanopores, it is still difficult to form stable channels on the plasma membrane of live cells by DNA nanopores. As a result, it significantly limits the applications of DNA nanopores in vivo. Thus, in this work, we have constructed ethane-phosphorothioate (PPT) groups modified DNA nanopores (E-DNA nanopores) to simulate biological channels for the transmembrane transport of small molecules. The E-DNA nanopores were found to be more hydrophobic and stable to anchor at the plasma membrane of live cells for a longer time window for subsequent transmembrane transport after the modification of ethane-PPT groups. The membrane-spanning E-DNA nanopores with a longer dwell time window could inspire the design of new DNA nanostructures and expand their biological applications including biosensing and sequencing, construction of artificial cells and regulation of transmembrane transport.
A plasma 3-marker microRNA biosignature distinguishes spinal tuberculosis from other spinal destructive diseases and pulmonary tuberculosis
Authors: Liang Qiang , Jin Weidong , Huang Zhigang , Yin Huquan , Liu Shengchun , Liu Liehua , Song Xiangwei , Wang Zili , Fei Jun
Published: March 2023

Accurate spinal tuberculosis (TB) diagnosis is of utmost importance for adequately treating and managing the disease. Given the need for additional diagnostic tools, this study aimed to investigate the utility of host serum miRNA biomarkers for diagnosing and distinguishing spinal tuberculosis (STB) from pulmonary tuberculosis (PTB) and other spinal diseases of different origins (SDD). For a case-controlled investigation, a total of 423 subjects were voluntarily recruited, with 157 cases of STB, 83 cases of SDD, 30 cases of active PTB, and 153 cases of healthy controls (CONT) in 4 clinical centers. To discover the STB-specific miRNA biosignature, a high-throughput miRNA profiling study was performed in the pilot study with 12 cases of STB and 8 cases of CONT using the Exiqon miRNA PCR array platform. A bioinformatics study identified that the 3-plasma miRNA combination (hsa-miR-506-3p, hsa-miR-543, hsa-miR-195-5p) might serve as a candidate biomarker for STB. The subsequent training study developed the diagnostic model using multivariate logistic regression in training data sets, including CONT(n=100) and STB (n=100). Youden’s J index determined the optimal classification threshold. Receiver Operating Characteristic (ROC) curve analysis showed that 3-plasma miRNA biomarker signatures have an area under the curve (AUC) = 0.87, sensitivity = 80.5%, and specificity = 80.0%. To explore the possible potential to distinguish spinal TB from PDB and other SDD, the diagnostic model with the same classification threshold was applied to the analysis of the independent validation data set, including CONT(n=45), STB(n=45), brucellosis spondylitis (BS, n=30), PTB (n=30), spinal tumor (ST, n=30) and pyogenic spondylitis (PS, n=23). The results showed diagnostic model based on three miRNA signatures could discriminate the STB from other SDD groups with sensitivity=80%, specificity=96%, Positive Predictive Value (PPV)=84%, Negative Predictive Value (NPV)=94%, the total accuracy rate of 92%. These results indicate that this 3-plasma miRNA biomarker signature could effectively discriminate the STB from other spinal destructive diseases and pulmonary tuberculosis. The present study shows that the diagnostic model based on 3-plasma miRNA biomarker signature (hsa-miR-506-3p, hsa-miR-543, hsa-miR-195-5p) may be used for medical guidance to discriminate the STB from other spinal destructive disease and pulmonary tuberculosis.
pH-activated antibiofilm strategies for controlling dental caries
Authors: Wang Xiuqing , Li Jingling , Zhang Shujun , Zhou Wen , Zhang Linglin , Huang Xiaojing
Published: March 2023

Dental biofilms are highly assembled microbial communities surrounded by an extracellular matrix, which protects the resident microbes. The microbes, including commensal bacteria and opportunistic pathogens, coexist with each other to maintain relative balance under healthy conditions. However, under hostile conditions such as sugar intake and poor oral care, biofilms can generate excessive acids. Prolonged low pH in biofilm increases proportions of acidogenic and aciduric microbes, which breaks the ecological equilibrium and finally causes dental caries. Given the complexity of oral microenvironment, controlling the acidic biofilms using antimicrobials that are activated at low pH could be a desirable approach to control dental caries. Therefore, recent researches have focused on designing novel kinds of pH-activated strategies, including pH-responsive antimicrobial agents and pH-sensitive drug delivery systems. These agents exert antibacterial properties only under low pH conditions, so they are able to disrupt acidic biofilms without breaking the neutral microenvironment and biodiversity in the mouth. The mechanisms of low pH activation are mainly based on protonation and deprotonation reactions, acids labile linkages, and H+-triggered reactive oxygen species production. This review summarized pH-activated antibiofilm strategies to control dental caries, concentrating on their effect, mechanisms of action, and biocompatibility, as well as the limitation of current research and the prospects for future study.
Recruitment of heterologous substrates by bacterial secretion systems for transkingdom translocation
Authors: Guzmán-Herrador Dolores L. , Fernández-Gómez Andrea , Llosa Matxalen
Published: March 2023

Bacterial secretion systems mediate the selective exchange of macromolecules between bacteria and their environment, playing a pivotal role in processes such as horizontal gene transfer or virulence. Among the different families of secretion systems, Type III, IV and VI (T3SS, T4SS and T6SS) share the ability to inject their substrates into human cells, opening up the possibility of using them as customized injectors. For this to happen, it is necessary to understand how substrates are recruited and to be able to engineer secretion signals, so that the transmembrane machineries can recognize and translocate the desired substrates in place of their own. Other factors, such as recruiting proteins, chaperones, and the degree of unfolding required to cross through the secretion channel, may also affect transport. Advances in the knowledge of the secretion mechanism have allowed heterologous substrate engineering to accomplish translocation by T3SS, and to a lesser extent, T4SS and T6SS into human cells. In the case of T4SS, transport of nucleoprotein complexes adds a bonus to its biotechnological potential. Here, we review the current knowledge on substrate recognition by these secretion systems, the many examples of heterologous substrate translocation by engineering of secretion signals, and the current and future biotechnological and biomedical applications derived from this approach.
Scene-object semantic incongruity across stages of processing: From detection to identification and episodic encoding
Authors: Ortiz-Tudela Javier , Jiménez Luis , Lupiáñez Juan
Published: March 2023

Visual processes are assumed to be affected by scene-object semantics throughout the stream of processing, from the earliest processes of conscious object detection to the later stages of object identification and memory encoding. However, very few studies have jointly explored these processes in a unified setting. In this study, we build upon a change detection task to assess the influence of semantic congruity between scenes and objects across three processing stages, as indexed through measures of conscious detection, object identification, and delayed recognition. Across four experiments, we show that semantically incongruent targets are easier to detect than their congruent counterparts, but that the latter are better identified and recognized in a surprise memory test. In addition, we used eye-tracking measures, in conjunction with these three behavioral indexes, to further understand the locus of the advantage observed in each case. The results indicate that (i) competition with other congruent objects modulates the effects of congruity on target detection, but it does not affect identification nor recognition memory, (ii) the detection cost of scene-congruent targets is mediated by earlier fixations on incongruent targets, (iii) neither fixation times, dwell times, nor pupil dilatation are related to the effects obtained in identification and recognition; and (iv) even though congruent targets are both better identified and remembered, the recognition benefit does not depend on the identification demands. The transversal approach taken in this study represents a challenging but exciting perspective that holds the potential to build bridges over the seemingly different but related fields of conscious detection, semantic identification, and episodic memory.
Thawing plateau time indicating the duration of phase transition from ice to water is the strongest predictor for long-term durable pulmonary vein isolation after cryoballoon ablation for atrial fibrillation—Data from the index and repeat procedures
Authors: Shi Li-Bin , Wollner Kristian , Chu Song-Yun , Wang Yu-Chuan , Solheim Eivind , Schuster Peter , Chen Jian
Published: March 2023

Introduction

This study aimed to clarify the relationship between the durability of pulmonary vein (PV) isolation and the time of phase transition from ice to water indicated by thawing plateau time in a cryoballoon ablation for atrial fibrillation (AF).

Methods and results

In this retrospective study, 241 PVs from 71 patients who underwent a repeat AF ablation 526 (IQR: 412, 675) days after a cryoballoon ablation were analyzed. Reconnection was observed in 101 (41.9%) PVs of 53 patients (74.6%). Thawing plateau time (TimeTP) was defined as the time from 0°C to 10°C inside the balloon in the thawing period. Durable PV isolation was associated with significantly longer TimeTP compared with PV reconnection (26.0 vs. 11.0 s, P < 0.001). The proportion of durable PV isolations increased with TimeTP in a dose-proportional manner. The cut point for PV reconnection was TimeTP <15 s with a positive predictive value of 82.1% (sensitivity = 63.4%, specificity = 90.0%) while for durable PV isolation the cut point was TimeTP >25 s with a positive predictive value of 84.6% (sensitivity = 55.0%, specificity = 86.1%). In the analysis of multivariable logistic regression, location of PV reconnection (P < 0.01), TimeTP (P < 0.05) and thawing plateau integral (P < 0.01) were shown as independent predictors for durable PV isolation.

Conclusion

TimeTP is an independent predictor for the durability of PV isolation, and it presents in a dose-proportional manner. TimeTP <15 s predicts long-term reconnection while TimeTP >25 s predicts durable PV isolation.
Age-related reference intervals for ambulatory electrocardiographic parameters in healthy individuals
Authors: Hashimoto Kenichi , Harada Naomi , Kimata Motohiro , Kawamura Yusuke , Fujita Naoya , Sekizawa Akinori , Ono Yosuke , Obuchi Yasuhiro , Takayama Tadateru , Kasamaki Yuji , Tanaka Yuji
Published: March 2023

Background

The advent of novel monitoring technologies has dramatically increased the use of ambulatory electrocardiography (AECG) devices. However, few studies have conducted detailed large-scale investigations on the incidence of arrhythmias over 24 h, especially ectopy, in healthy individuals over a wide age range.

Objectives

This study aimed to investigate the incidence of arrhythmias detected using AECG and associated factors, in healthy individuals, over a wide age range.

Methods

In this cross-sectional study, we performed AECG on 365 healthy volunteers (median [interquartile range]: 48 [36, 67], 20–89 years, 165 men) under free-running conditions for 24 h. Ultrasonic echocardiography and heart rate variability analysis were performed to explore the factors associated with the incidence of arrhythmias.

Results

The 97.5th percentile of single ventricular ectopy (VE) was 149/day, 254/day, and 1,682/day in the 20–39-, 40–59- and 60–89-year age groups, respectively; that of single supraventricular ectopy (SVE) was 131/day, 232/day, and 1,063/day, respectively. Multivariate analysis revealed that aging was the only independent significant factor influencing the frequency of VE (β = 0.207, P = 0.001). Age (β = 0.642, P < 0.001), body mass index (BMI) (β = −0.112, P = 0.009), and the root mean square of successive differences in RR intervals (β = 0.097, P = 0.035) were factors significantly associated with SVE frequency.

Conclusions

Age-specific reference intervals of VE and SVE in a large population of healthy participants over a wide age range were generated. VE and SVE increased with age; SVE was influenced by BMI and the aging-induced decrease in parasympathetic tone activity.
Deletion of the murine ortholog of human 9p21.3 locus promotes atherosclerosis by increasing macrophage proinflammatory activity
Authors: Kettunen Sanna , Ruotsalainen Anna-Kaisa , Örd Tiit , Suoranta Tuisku , Heikkilä Janne , Kaikkonen Minna U. , Laham-Karam Nihay , Ylä-Herttuala Seppo
Published: March 2023

Background

Several genome-wide association studies have reported a risk locus for coronary artery disease (CAD) in the 9p21. 3 chromosomal region. This region encodes a lncRNA in the INK4 locus (ANRIL) and its genetic variance has a strong association with CAD, but its mechanisms in atherogenesis remain unclear.

Objectives

This study aimed to investigate the role of the murine ortholog of human 9p21.3 locus in atherogenesis in hypercholesterolemic mice.

Methods

Murine 9p21.3 ortholog knockout mice (Chr4Δ70kb/Δ70kb) were crossbred with Ldlr−/−ApoB100/100 mice, and atherosclerotic plaque size and morphology were analyzed on a standard or a high-fat diet (HFD). The hematopoietic cell-specific effect of Chr4Δ70kb/Δ70kb on atherosclerotic plaque development was studied via bone marrow (BM) transplantation, where Chr4Δ70kb/Δ70kb or wild-type BM was transplanted into Ldlr−/−ApoB100/100 mice. The role of Chr4Δ70kb/Δ70kb in macrophage M1/M2 polarization was studied. In addition, single-cell sequencing data from human and mouse atheroma were analyzed to show the expression profiles of ANRIL and its murine equivalent, Ak148321, in the plaques.

Results

Both systemic and hematopoietic Chr4Δ70kb/Δ70kb increased atherosclerosis in Ldlr−/−ApoB100/100 mice after 12 weeks of HFD. The systemic Chr4Δ70kb/Δ70kb also elevated the number of circulating leukocytes. Chr4Δ70kb/Δ70kb BMDMs showed enhanced M1 polarization in vitro. Single-cell sequencing data from human and mouse atheroma revealed that ANRIL and Ak148321 were mainly expressed in the immune cells.

Conclusion

These data demonstrate that both systemic and BM-specific deletion of the murine 9p21.3 risk locus ortholog promotes atherosclerosis and regulates macrophage pro-inflammatory activity, suggesting the inflammation-driven mechanisms of the risk locus on atherogenesis.
Case report: Multi-site perfusion strategy for type A acute aortic dissection complicated with cerebral malperfusion
Authors: Besa Santiago , Castelli Fiorenza , Garrido-Olivares Luis , González Rodrigo , Marine Leopoldo , Becker Pedro
Published: March 2023

Acute type A dissection presenting with cerebral malperfusion has high morbidity and mortality. Given the complexity of underlying vascular involvement, it is a challenging clinical scenario. Many of these patients are not deemed surgical candidates. If surgery is considered, it often requires complex aortic arch and neck vessel reconstruction. We present a 48-year-old male with an acute type A aortic dissection that presented with paraplegia and decreased level of consciousness. A Computed Tomography showed occlusion of both common carotid arteries. He was successfully treated with a multi-site perfusion strategy and a Hybrid Frozen Elephant Trunk graft to achieve fast restoration of the cerebral circulation and minimize brain ischemia and permanent neurological damage. From this case, we learn that aggressive arch and neck vessel reconstruction supported by multi-site perfusion could help improve mortality and neurological outcomes in selected patients.
Identification of immune-related hub genes and analysis of infiltrated immune cells of idiopathic pulmonary artery hypertension
Authors: Chen Yubin , Ouyang Tianyu , Yin Yue , Fang Cheng , Tang Can-e , Jiang Longtan , Luo Fanyan
Published: March 2023

Objectives

Idiopathic pulmonary artery hypertension (IPAH) is a rare but life-threaten disease. However, the mechanism underlying IPAH is unclear. In this study, underlying mechanism, infiltration of immune cells, and immune-related hub genes of IPAH were analyzed via bioinformatics.

Methods

GSE15197, GSE48149, GSE113439, and GSE117261 were merged as lung dataset. Weighted gene correlation network analysis (WGCNA) was used to construct the co-expression gene networks of IPAH. Gene Ontology and pathway enrichment analysis were performed using DAVID, gene set enrichment analysis (GSEA), and gene set variation analysis (GSVA). Infiltration of immune cells in lung samples was analyzed using CIBERSORT. GSE22356 and GSE33463 were merged as peripheral blood mononuclear cells (PBMCs) dataset. Immune-related differentially expressed genes (IRDEGs) of lung and PBMCs dataset were analyzed. Based on the intersection between two sets of IRDEGs, hub genes were screened using machine learning algorithms and validated by RT-qPCR. Finally, competing endogenous RNA (ceRNA) networks of hub genes were constructed.

Results

The gray module was the most relevant module and genes in the module enriched in terms like inflammatory and immune responses. The results of GSEA and GSVA indicated that increasement in cytosolic calcium ion, and metabolism dysregulation play important roles in IPAH. The proportions of T cells CD4 memory resting and macrophage M1 were significantly greater in IPAH group, while the proportions of monocytes and neutrophils were significantly lower in IPAH group. IRDEGs of two datasets were analyzed and the intersection between two set of IRDEGs were identified as candidate hub genes. Predictive models for IPAH were constructed using data from PBMCs dataset with candidate hub genes as potential features via LASSO regression and XGBoost algorithm, respectively. CXCL10 and VIPR1 were identified as hub genes and ceRNA networks of CXCL10 was constructed.

Conclusion

Inflammatory response, increasement in cytosolic calcium ion, and metabolism dysregulation play important roles in IPAH. T cells CD4 memory resting and macrophage M1 were significantly infiltrated in lung samples from patients with IPAH. IRDEGs of lung dataset and PBMCs dataset were analyzed, and CXCL10 and VIPR1 were identified as hub genes.
Precision and genomic medicine for dilated and hypertrophic cardiomyopathy
Authors: Nomura Seitaro , Ono Minoru
Published: March 2023

Cardiomyopathy develops through an interaction of genetic and environmental factors. The clinical manifestations of both dilated cardiomyopathy and hypertrophic cardiomyopathy are diverse, but genetic testing defines the causative genes in about half of cases and can predict clinical prognosis. It has become clear that cardiomyopathy is caused not only by single rare variants but also by combinations of multiple common variants, and genome-wide genetic research is important for accurate disease risk assessment. Single-cell analysis research aimed at understanding the pathophysiology of cardiomyopathy is progressing rapidly, and it is expected that genomic analysis and single-cell molecular profiling will be combined to contribute to more detailed stratification of cardiomyopathy.
Probabilistic approach to Gramian inversion of multiphysics data
Authors: Zhdanov Michael S. , Jorgensen Michael , Tao Mo
Published: March 2023

We consider a probabilistic approach to the joint inversion of multiphysics data based on Gramian constraints. The multiphysics geophysical survey represents the most effective technique for geophysical exploration because different physical data reflect distinct physical properties of the various components of the geological system. By joint inversion of the multiphysics data, one can produce enhanced subsurface images of the physical properties distribution, which improves our ability to explore natural resources. One powerful method of joint inversion is based on Gramian constraints. This technique enforces the relationships between different model parameters during the inversion process. We demonstrate that the Gramian can be interpreted as a determinant of the covariance matrix between different physical models representing the subsurface geology in the framework of the probabilistic approach to inversion theory. This interpretation opens the way to use all the power of the modern probability theory and statistics in developing novel methods for joint inversion of the multiphysics data. We apply the developed joint inversion methodology to inversion of gravity gradiometry and magnetic data in the Nordkapp Basin, Barents Sea to image salt diapirs.
Analysis of the spillover characteristics of cooling effect in an urban park: A case study in Zhengzhou city
Authors: Gao Yu , Pan Huifeng , Tian Li
Published: March 2023

Urban parks include water bodies, non-hardened ground, and a variety of vegetation, whose shading and evaporation effects form a “park cold island.” A park effectively cools the surrounding environment through heat exchange. This phenomenon is called spillover of park cooling effect (PCS) and plays an important role in regulating the microclimate of cities. Although PCS was extensively documented in previous studies, the effects of park landscape features on PCS still need to be further explored, especially in China with rapid urban expansion. The severe scarcity of urban land resources heightens the necessity to clarify the relationship between park patch size and cooling efficiency. Therefore, in this study, we chose Zhengzhou city (the capital of Henan province, in the central region of China) as an example, which has experienced rapid urban land expansion and urban population and high spatiotemporal aggregation of heatwaves–drought–rainstorms. We used Landsat 8 imagery and high score data in the summer of 2019 to retrieve the characteristics of land surface temperature (LST), and then we extracted 36 city parks and identified the spillover distance of the park cooling effect (PCSD), spillover strength of the park cooling effect (PCSS), spillover rate of the park cooling effect (PCSR), and 11 park landscape indexes. We calculated the area threshold when a park achieves the highest cooling efficiency, and the PCS characteristics of each park also were quantified. The results showed that the average LST of urban parks was 2.3°C lower than that of the entire study area; PCSS was 4.61°C at a maximum; PCSD was between 31 and 370 m, and the average value was 179 m; the average PCSR was 0.957°C/100 m. For the relationship of PCS with the 11 park landscape indexes, we found park area (AREA), park green space area (AREAg), and park perimeter (PERIM) contributed the most to PCS. Shape of impervious surface (LSIi) and percentage of water body (Pw) also significantly contributed to PCS. The ratio of perimeter to area (PARA), edge density (ED), edge density of the green space (EDg), and patch density (PD) were significantly and negatively correlated with PCS. For the relationship between park area and PCS, the result was that the park with an area of 6–8 ha and an internal green area of not less than 5–6 ha has the highest PCS effect. This study can expand our scientific understanding on the influences of park landscape characteristics on PCS and provide a scientific basis for formulating reasonable and effective urban parks and spatial layout planning to cope with the urban heat island effect.
A multi-millennial record of rock glacier ice chemistry (Lazaun, Italy)
Authors: Nickus Ulrike , Thies Hansjörg , Krainer Karl , Lang Kathrin , Mair Volkmar , Tonidandel David
Published: March 2023

Active rock glaciers—known as mixtures of unconsolidated debris with interstitial ice, ice lenses or a core of massive ice—are widespread indicators of mountain permafrost. The age of a frozen rock glacier core in the Central European Alps (Lazaun, Italy) was dated to about 10,000 years. Here we report on the chemical composition of the frozen Lazaun core. The ice containing part of the core extended from about 2.8 m down to 24 m depth and consisted of two lobes—both a mix of ice and debris, separated by more than 3 m thick almost ice-free layer. The two lobes of the core showed layers of high solute content and peak values of electrical conductivity exceeding 1,000 μS/cm, but they differed in acidity and metal concentration. High acidity (minimum pH of 4.15) and high levels of elements like nickel, cobalt, zinc, manganese, iron and aluminum characterized the upper lobe, while neutral to alkaline pH and low metal values prevailed in the bottom lobe. We attributed solutes accumulated in the ice matrix to the weathering of bedrock minerals, with peak values favored by the oxidation of pyrite, or by an enhanced reactive surface area in fine-grained sediment layers. The chemical composition of the ice core also revealed signals of prehistoric atmospheric deposition from different sources including wood combustion, metal ore mining, and large volcanic eruptions (Thera, Aniakchak II). To our knowledge, this is the first study that presents the chemical stratigraphy of an entire rock glacier ice core.
Assessing and comparing alternative certification programs: The teacher-classroom-community model
Authors: Dori Yehudit Judy , Goldman Daphne , Shwartz Gabriella , Lavie-Alon Nirit , Sarid Ariel , Tal Tali
Published: March 2023

Alternative certification programs (ACPs) differ from traditional teacher certification programs in their target populations, duration, tools they employ, their pedagogy, and subject matter curricula. Given the acute shortage of excellent teachers, especially in STEM, significant efforts and resources are invested in ACPs so they prepare highly qualified teachers. Yet, novice teachers face difficulties during their initial integration into the school system. To better understand the state of affairs, we investigated and compared the integration into the school system of graduates of five major Israeli ACPs that are tailored for diverse student-teacher target audiences. The study goals were to (1) investigate and compare the integration of graduates of the five ACPs into the teaching profession with respect to five teacher-related aspects: (a) self-efficacy, (b) commitment to the teaching profession, (c) challenges encountered, (d) leadership roles, and (e) teamwork; (2) identify ACP characteristics that support the graduates’ integration into the teaching profession. The teacher-classroom-community model we propose, holistically connects three aspects: affective – the teacher, the teaching profession – the classroom, and peer interaction and leadership – the school community. The model provides a common language for comparing how the different ACPs prepared their graduates toward the teaching profession. The model is instrumental for identifying ACP characteristics that support graduates’ integration into teaching and facilitating ACP evaluation by connecting several aspects of teachers’ professional lives. The study employed a mixed-methodology in which 506 graduates responded to a closed- and open-ended questionnaire and 71 interviews were conducted with graduates (novice teachers), ACP directors, school principals and mentor teachers. The findings depict a complex picture that reflects the different ACPs’ characteristics targeted at diverse audiences. For example, graduates of STEM-oriented programs perceive the different kinds of knowledge, including content knowledge, pedagogical knowledge, and pedagogical content knowledge, as most important to their roles in schools. They undertake fewer roles, and the ones they do assume are discipline-related. Graduates of the more social-leadership-oriented programs identify developing leadership skills as most beneficial and they undertake more leadership-related roles. The research highlights key aspects that teacher education leaders should consider and use for self-evaluation of their ACPs. The strength of this study stems from proposing and applying the teacher-classroom-community model for evaluating teacher certification programs in several contexts and for diverse groups along with their integration into schools.
Visualization analysis of feminist translation research in China (2002–2021) based on CiteSpace
Authors: Sun Fei , Wang Xiaochen , Li Hongbin , Wang Siying
Published: March 2023

Based on 601 articles on feminist translation theory published in China National Knowledge Infrastructure (CNKI) in the last 20 years from 2002 to 2021, this article draws a scientific knowledge map and makes a visual analysis of the research articles on feminist translation theory in China using CiteSpace as a visual tool. It was found that (1) both the cooperation between researchers and between research institutions in the domain of feminist translation research in China was not strong; (2) the focus of feminist translation research in China is mainly on feminism, feminist translation theory, feminist translation, and translation; (3) according to the map of the time zone and keywords mutation, feminist translation research in China is constantly moving toward a decline after fast development.
The construct validity of the main student selection tests for medical studies in Germany
Authors: Levacher Julie , Koch Marco , Stegt Stephan J. , Hissbach Johanna , Spinath Frank M. , Escher Malvin , Becker Nicolas
Published: March 2023

Standardized ability tests that are associated with intelligence are often used for student selection. In Germany two different admission procedures to select students for medical studies are used simultaneously; the TMS and the HAM-Nat. Due to this simultaneous use of both a detailed analysis of the construct validity is mandatory. Therefore, the aim of the study is the construct validation of both selection procedures by using data of 4,528 participants (Mage = 20.42, SD = 2.74) who took part in a preparation study under low stakes conditions. This study compares different model specifications within the correlational structure of intelligence factors as well as analysis the g-factor consistency of the admission tests. Results reveal that all subtests are correlated substantially. Furthermore, confirmatory factor analyses demonstrate that both admission tests (and their subtests) are related to g as well as to a further test-specific-factor. Therefore, from a psychometric point of view, the simultaneous use of both student selection procedures appears to be legitimate.
Is there any association between early trimester Triglyceride–glucose index and incidence of hypertensive disorder of pregnancy and adverse pregnancy outcomes?
Authors: Pan Yali , Zou Su , Xu Yingjia , Di Ruomin , Gu Huafen , Wang Zhangsheng , Wei Xiang , Yang Chenxi , Zhang Gaofeng
Published: March 2023

Background

Insulin resistance (IR) is a normal feature of pregnancy and plays a crucial role in the pathophysiology of hypertensive disorder of pregnancy (HDP). The triglyceride-glucose index (TyG index) has been shown as a simple and reliable alternative IR marker. This work aimed to investigate the association between the TyG index and the incidence of HDP and adverse pregnancy outcomes.

Methods

From January 2016 to December 2018, 289 women with HDP and 861 women without HDP were recruited at Shanghai Fifth People’s Hospital, Fudan University to determine the relationship between the TyG index and the incidence of HDP and adverse pregnancy outcomes.

Results

In the case-control study, the incidence of HDP was found to be significantly associated with the TyG index. Moreover, logistic regression indicated that the TyG index is an independent risk factor for HDP development and incidence of low birth weight (LBW) and fetal distress. In the cohort study, the results showed that the TyG index increased, there was a stepwise increase in HDP incidence, SBP, and DBP levels one week before delivery as well as in LBW and fetal distress incidence. The early trimester TyG index was positively associated with pre-pregnancy BMI, systolic blood pressure (SBP), and diastolic blood pressure (DBP) one week before delivery. Spline regression showed that there was a significant linear association between HDP incidence and early trimester TyG index when it was >8.5.

Conclusions

This work suggested that the early trimester TyG index was closely associated with the development of HDP and adverse pregnancy outcomes.
Growth hormone supplementation ameliorates blastocyst euploidy rates and improves pregnancy outcomes in women undergoing preimplantation genetic testing for aneuploidy cycles
Authors: Guo Qingqing , Liu Peihao , Zhou Wei , Xia Mingdi , Li Jing , Lu Juanjuan , Ma Jin-Long , Chen Zi-Jiang , Yan Junhao
Published: March 2023

Background

Growth hormone (GH) supplementation has been shown to improve oocyte quality and live birth, but few studies have examined whether GH can reduce embryonic aneuploidy. Chromosomal abnormalities in preimplantation embryos have been regarded as the principal cause of implantation failure and miscarriage, and an increased percentage of aneuploid embryos has been observed in patient cohorts with unexplained recurrent pregnancy loss (RPL), recurrent implantation failure (RIF), and advanced maternal age.

Methods

This prospective cohort study was conducted on women whose previous PGT-A cycle ended up with no transferrable blastocysts, or the aneuploidy rate was above 50% and no live birth was acquired. The participants were divided into GH co-treatment and comparison groups according to whether GH was administered in the subsequent PGT-A cycle. In addition, within the GH co-treatment group, the previous failed cycle constituted the self-control group.

Results

208 women were recruited in the study (GH co-treatment group: 96 women, comparison group: 112 women). Compared to the self-control and comparison groups, the rate of euploid blastocysts was significantly higher in the GH co-treatment group (GH vs self-control: 32.00% vs 9.14%, odds ratio [OR]: 4.765, 95% confidence interval [CI]: 2.420–9.385, P < 0.01; GH vs comparison: 32.00% vs. 21.05%, OR: 1.930, 95% CI: 1.106–3.366, P = 0.021), and their frozen embryo transfers resulted in more pregnancies and live births. In the subgroup analysis, for the <35 and 35-40 years groups, the euploidy rate in the GH co-treatment group was significantly higher than those in the self-control and comparison groups, but in the >40 years group, there was no difference in euploidy rate.

Conclusion

Our study presents preliminary evidence that GH supplementation may ameliorate blastocyst aneuploidy and improve pregnancy outcomes in women who have previously experienced pregnancy failures along with high aneuploidy rates, particularly in those younger than 40 years. Therefore, the use of GH in such women should be considered. However, considering the limited sample size and mixed indications for PGT-A, further scientific research on the underlying mechanism as well as clinical trials with larger sample sizes are needed to confirm the effects and optimal protocols.
Diabetic cardiomyopathy: The role of microRNAs and long non-coding RNAs
Authors: Macvanin Mirjana T. , Gluvic Zoran , Radovanovic Jelena , Essack Magbubah , Gao Xin , Isenovic Esma R.
Published: March 2023

Diabetes mellitus (DM) is on the rise, necessitating the development of novel therapeutic and preventive strategies to mitigate the disease’s debilitating effects. Diabetic cardiomyopathy (DCMP) is among the leading causes of morbidity and mortality in diabetic patients globally. DCMP manifests as cardiomyocyte hypertrophy, apoptosis, and myocardial interstitial fibrosis before progressing to heart failure. Evidence suggests that non-coding RNAs, such as long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), regulate diabetic cardiomyopathy-related processes such as insulin resistance, cardiomyocyte apoptosis and inflammation, emphasizing their heart-protective effects. This paper reviewed the literature data from animal and human studies on the non-trivial roles of miRNAs and lncRNAs in the context of DCMP in diabetes and demonstrated their future potential in DCMP treatment in diabetic patients.
Assessment of the association between genetic factors regulating thyroid function and microvascular complications in diabetes: A two-sample Mendelian randomization study in the European population
Authors: Li Hongdian , Li Mingxuan , Dong Shaoning , Zhang Sai , Dong Ao , Zhang Mianzhi
Published: March 2023

Background

Observational studies have identified a possible link between thyroid function and diabetic microangiopathy, specifically in diabetic kidney disease (DKD) and diabetic retinopathy (DR). However, it is unclear whether this association reflects a causal relationship.

Objective

To assess the potential direct effect of thyroid characteristics on DKD and DR based on Mendelian randomization (MR).

Methods

We conducted an MR study using genetic variants as an instrument associated with thyroid function to examine the causal effects on DKD and DR. The study included the analysis of 4 exposure factors associated with thyroid hormone regulation and 5 outcomes. Genomewide significant variants were used as instruments for standardized freethyroxine (FT4) and thyroid-stimulating hormone (TSH) levels within the reference range, standardized free triiodothyronine (FT3):FT4 ratio, and standardized thyroid peroxidase antibody (TPOAB) levels. The primary outcomes were DKD and DR events, and secondary outcomes were estimated glomerular filtration rate (eGFR), urinary albumin-to-creatinine ratio (ACR) in diabetes, and proliferative diabetic retinopathy (PDR). Satisfying the 3 MR core assumptions, the inverse-variance weighted technique was used as the primary analysis, and sensitivity analysis was performed using MR-Egger, weighted median, and MR pleiotropy residual sum and outlier techniques.

Results

All outcome and exposure instruments were selected from publicly available GWAS data conducted in European populations. In inverse-variance weighted random-effects MR, gene-based TSH with in the reference range was associated with DKD (OR 1.44; 95%CI 1.04, 2.41; P = 0.033) and eGFR (β: -0.031; 95%CI: -0.063, -0.001; P = 0.047). Gene-based increased FT3:FT4 ratio, decreased FT4 with in the reference range were associated with increased ACR with inverse-variance weighted random-effects β of 0.178 (95%CI: 0.004, 0.353; P = 0.046) and -0.078 (95%CI: -0.142, -0.014; P = 0.017), respectively, and robust to tests of horizontal pleiotropy. However, all thyroid hormone instruments were not associated with DR and PDR at the genetic level.

Conclusion

In diabetic patients, an elevated TSH within the reference range was linked to a greater risk of DKD and decreased eGFR. Similarly, decreased FT4 and an increased FT3:FT4 ratio within the reference range were associated with increased ACR in diabetic patients. However, gene-based thyroid hormones were not associated with DR, indicating a possible pathway involving the thyroid-islet-renal axis. However, larger population studies are needed to further validate this conclusion.
Serum uric acid: A risk factor for right ventricular dysfunction and prognosis in heart failure with preserved ejection fraction
Authors: Deng Xiang-liang , Yi Han-wen , Xiao Jin , Zhang Xiao-fang , Zhao Jin , Sun Min , Wen Xue-song , Liu Zhi-qiang , Gao Lei , Li Zi-yang , Ge Ping , Yu Qi , Zhang Dong-ying
Published: March 2023

Background

Hyperuricemia and right ventricular dysfunction (RVD) are both widespread in heart failure with preserved ejection fraction (HFpEF) patients. RVD is associated with a poor prognosis in HFpEF. The correlation between serum uric acid (UA) levels and right ventricular function is unclear. The prognostic performance of UA in patients with HFpEF needs further validation.

Methods and results

A total of 210 patients with HFpEF were included in the study and divided into two groups according to UA level: the normal UA group (≤7 mg/dl) and the high UA group (>7 mg/dl). The variables examined included clinical characteristics, echocardiography, and serum biochemical parameters. Right ventricular function was assessed by tricuspid annular plane systolic excursion (TAPSE) and tricuspid annular peak systolic velocity (TAPSV). Baseline characteristics were compared between the two groups, and the correlation between baseline UA and RVD was assessed using multifactorial binary logistic regression. Kaplan–Meier curves were used to describe all-cause mortality and heart failure readmission. Results showed that right ventricular function parameters were worse in the high UA group. After adjusting for UA, left ventricular posterior wall thickness (LVPWT), N-terminal B-type natriuretic peptide (NT-proBNP), atrial fibrillation (AF), and low-density lipoprotein cholesterol (LDL-C), UA (odds ratio = 2.028; p < 0.001) was independently associated with RVD, and UA >7 mg/dl (HR = 2.98; p < 0.001) was associated with heart failure readmission in patients with HFpEF.

Conclusion

Elevated serum UA is closely associated with RVD and significantly associated with the heart failure readmission rate in patients with HFpEF.
Studies related to osteosarcoma and metabolism from 1990 to 2022: A visual analysis and bibliometric study
Authors: Shao Zhuce , Bi Shuxiong
Published: March 2023

Background

Osteosarcoma is the most common primary bone tumor, its high incidence of metastasis and poor prognosis have led to a great deal of concern for osteosarcoma. In many cancer types, metabolic processes are important for tumor growth progression, so interfering with the metabolic processes of osteosarcoma may be a therapeutic option to stall osteosarcoma progression. A key mechanism of how metabolic processes contribute to the growth and survival of various cancers, including osteosarcoma, is their ability to support tumor cell metabolism. Research related to this field is a direction of great importance and potential. However, to our knowledge, no bibliometric studies related to this field have been published, and we will fill this research gap.

Methods

Publications were retrieved on January 1, 2023 from the 1990-2022 Science Citation Index of the Web of Science Core Collection. The Bibliometrix package in R software, VOSviewer and CiteSpace software were used to analyze our research directions and to visualize global trends and hotspots in osteosarcoma and metabolism related research.

Results

Based on the search strategy, 833 articles were finally filtered. In this area of research related to osteosarcoma metabolism, we found that China, the United States and Japan are the top 3 countries in terms of number of articles published, and the journals and institutions that have published the most research in this area are Journal of bone and mineral research, Shanghai Jiao Tong University. In addition, Baldini, Nicola, Reddy, Gs and Avnet, Sofia are the top three authors in terms of number of articles published in studies related to this field. The most popular keywords related to the field in the last 30 years are “metabolism” and “expression”, which will guide the possible future directions of the field.

Conclusion

We used Bibliometrix, VOSviewer, and Citespace to visualize and bibliometrically analyze the current status and possible future hotspots of research in the field of osteosarcoma metabolism. Possible future hotspots in this field may focus on the related terms “metabolism”, “expression”, and “migraation”.
Acetylcholine regulation of GnRH neuronal activity: A circuit in the medial septum
Authors: Shostak David M. , Constantin Stephanie , Flannery Jill , Wray Susan
Published: March 2023

In vertebrates, gonadotropin-releasing hormone (GnRH)-secreting neurons control fertility by regulating gonadotrophs in the anterior pituitary. While it is known that acetylcholine (ACh) influences GnRH secretion, whether the effect is direct or indirect, and the specific ACh receptor (AChR) subtype(s) involved remain unclear. Here, we determined 1) whether ACh can modulate GnRH cellular activity and 2) a source of ACh afferents contacting GnRH neurons. Calcium imaging was used to assay GnRH neuronal activity. With GABAergic and glutamatergic transmission blocked, subtype-specific AChR agonists and antagonists were applied to identify direct regulation of GnRH neurons. ACh and nicotine caused a rise in calcium that declined gradually back to baseline after 5-6 min. This response was mimicked by an alpha3-specific agonist. In contrast, muscarine inhibited GnRH calcium oscillations, and blocking M2 and M4 together prevented this inhibition. Labeling for choline acetyltransferase (ChAT) and GnRH revealed ChAT fibers contacting GnRH neurons, primarily in the medial septum (MS), and in greater number in females than males. ChAT positive cells in the MS are known to express p75NGFRs. Labeling for p75NGFR, ChAT and GnRH indicated that ChAT fibers contacting GnRH cells originate from cholinergic cells within these same rostral areas. Together, these results indicate that cholinergic cells in septal areas can directly regulate GnRH neurons.