bluuebunny/crossref_metadata_2025 huggingface dataset:
Venous Thromboembolism Management throughout the COVID-19 Era: Addressing Acute and Long-Term Challenges
Authors: Wu Maddalena Alessandra , Taino Alba , Facchinetti Pietro , Rossi Valentina , Ruggiero Diego , Berra Silvia , Blanda Giulia , Flor Nicola , Cogliati Chiara , Colombo Riccardo
Published: March 2024

Background: COVID-19 increases the risk of venous thromboembolism (VTE) through a complex interplay of mechanisms collectively referred to as immunothrombosis. Limited data exist on VTE challenges in the acute setting throughout a dynamic long-term follow-up of COVID-19 patients compared to non-COVID-19 patients. The aim of the study was to investigate acute and long-term management and complications in VTE patients with and without COVID-19. Methods: A prospective, observational, single-center cohort study on VTE patients followed from the acute care stage until 24 months post-diagnosis. Results: 157 patients, 30 with COVID-19-associated VTE and 127 unrelated to COVID-19, were enrolled. The mean follow-up was 10.8 (±8.9) months. COVID-19 patients had fewer comorbidities (1.3 ± 1.29 vs. 2.26 ± 1.68, p < 0.001), a higher proportion of pulmonary embolism at baseline (96.7% vs. 76.4%, p = 0.01), and had a lower probability of remaining on anticoagulant therapy after three months (p < 0.003). The most used initial therapy was low-molecular-weight heparin in 130/157 cases, followed by long-term treatment with direct oral anticoagulants in 123/157. Two (6.7%) COVID-19 vs. three (2.4%) non-COVID-19 patients (p = 0.243) had major hemorrhagic events, all of them within the first three months. Four (3.1%) non-COVID-19 patients had VTE recurrence after six months. Three (2.4%) non-COVID-19 patients developed chronic thromboembolic pulmonary hypertension. There were no fatalities among patients with COVID-19, compared to a mortality of 12/127 (9.4%) in the non-COVID-19 subgroup (p = 0.027). Discussion: Our study offers a comprehensive overview of the evolving nature of VTE management, emphasizing the importance of personalized risk-based approaches, including a limited course of anticoagulation for most COVID-19-associated VTE cases and reduced-dose extended therapy for high-risk subsets.
Immunohistochemical Evaluation of Renal Biopsy with Anti-PD1 and p53 to Solve the Dilemma between Platinum- and Pembrolizumab-Induced AKI: Case Report and Review
Authors: Mancianti Nicoletta , Tripodi Sergio Antonio , Pascucci Alessandra , Calatroni Marta , La Porta Edoardo , Guarnieri Andrea , Garosi Guido
Published: March 2024

Introduction: The combination therapy of platinum and pembrolizumab looks like a promising treatment in advanced non-small-cell lung cancer. However, both platinum-based chemotherapy and pembrolizumab can lead to AKI. AKI can occur due to acute tubular necrosis or interstitial nephritis. It is essential to identify the drug responsible for renal damage. For this purpose, we used new immunohistochemistry markers (p53 and anti-PD1 analysis). Case Description: A 77-year-old female patient with advanced non-small-cell lung cancer received the PD-1 inhibitor pembrolizumab and platinum-based chemotherapy carboplatin. The patient, after 60 days, experienced AKI. A kidney biopsy was performed, and two new immunohistochemical techniques for p53 (experimental markers of ATN from platinum) and anti-PDL1 (experimental markers of PD-1 inhibitors nephritis) were employed. Renal biopsies revealed severe tubular damage. No infiltration was detected, and the immunohistochemical assessment of PDL-1 was negative. The expression of p53 was positive. The renal biopsy suggested platinum-induced acute tubular necrosis. After discontinuing steroids and reducing carboplatin, the patient continued with pembrolizumab, and their renal function returned to normal within two months. Discussion: Combining checkpoint inhibitors and platinum-based therapies may result in AKI. The standard method of examining kidney tissue may not provide sufficient information about the effects of these drugs on the kidneys. To address this issue, we recommend incorporating an assessment of the analysis of the expression of PDL1 and p53. This personalized approach will help identify the best treatment option for the patient while ensuring the best possible cancer treatment plan.
Personalized Assessment of Mortality Risk and Hospital Stay Duration in Hospitalized Patients with COVID-19 Treated with Remdesivir: A Machine Learning Approach
Authors: Ramón Antonio , Bas Andrés , Herrero Santiago , Blasco Pilar , Suárez Miguel , Mateo Jorge
Published: March 2024

Background: Despite advancements in vaccination, early treatments, and understanding of SARS-CoV-2, its impact remains significant worldwide. Many patients require intensive care due to severe COVID-19. Remdesivir, a key treatment option among viral RNA polymerase inhibitors, lacks comprehensive studies on factors associated with its effectiveness. Methods: We conducted a retrospective study in 2022, analyzing data from 252 hospitalized COVID-19 patients treated with remdesivir. Six machine learning algorithms were compared to predict factors influencing remdesivir’s clinical benefits regarding mortality and hospital stay. Results: The extreme gradient boost (XGB) method showed the highest accuracy for both mortality (95.45%) and hospital stay (94.24%). Factors associated with worse outcomes in terms of mortality included limitations in life support, ventilatory support needs, lymphopenia, low albumin and hemoglobin levels, flu and/or coinfection, and cough. For hospital stay, factors included vaccine doses, lung density, pulmonary radiological status, comorbidities, oxygen therapy, troponin, lactate dehydrogenase levels, and asthenia. Conclusions: These findings underscore XGB’s effectiveness in accurately categorizing COVID-19 patients undergoing remdesivir treatment.
Effects of Dry Needling on Active Myofascial Trigger Points and Pain Intensity in Tension-Type Headache: A Randomized Controlled Study
Authors: Monti-Ballano Sofía , Márquez-Gonzalvo Sergio , Lucha-López María Orosia , Ferrández-Laliena Loreto , Vicente-Pina Lucía , Sánchez-Rodríguez Rocío , Tricás-Vidal Héctor José , Tricás-Moreno José Miguel
Published: March 2024

Tension-type headache is the most prevalent type of headache and is commonly associated with myofascial pain syndrome and the presence of active myofascial trigger points. This randomized controlled trial aimed to assess the impact of dry needling on the total number of active trigger points, pain intensity, and perceived clinical change in tension-type headache subjects. Thirty-two subjects were randomly assigned to the control and dry needling groups. The presence of active trigger points in 15 head and neck muscles, the headache intensity, and the perceived clinical change were evaluated. A single dry needling technique was administered at each active trigger point across three sessions. Significant differences were observed in the post-treatment measures favouring the dry needling group, including reductions in the headache intensity scores (p = 0.034) and the total number of active trigger points (p = 0.039). Moreover, significant differences in the perception of clinical change were found between the control and treatment groups (p = 0.000). Dry needling demonstrated positive effects in reducing the number of active trigger points and improving the short-term headache intensity in tension-type headache patients. A single dry needling session applied in the cranio-cervical area resulted in a self-perceived improvement compared to the control subjects.
Extra-Anogenital Giant Cutaneous Squamous Cell Carcinomas
Authors: Mateuszczyk Mateusz K. , Chlebicka Iwona , Łyko Magdalena , Maj Joanna , Szepietowski Jacek C.
Published: March 2024

Extra-anogenital giant cSCCs are rare but have worse outcomes compared to smaller tumors. Prompted by limited data, the authors conducted a retrospective study to gather more information about giant cSCCs to optimize clinical care. We identified seven cases of giant cSCCs from a review of cSCC cases treated in the Unit of Dermatosurgery between 2016 and 2022. Most patients were male (85.71%) with a mean age of 80.29 ± 12.22 years. UV radiation was the most common risk factor (five cases) followed by smoking (three cases) and hidradenitis suppurativa (one case). Most giant cases were located in the head area (71.4%) and the diameter of lesions ranged from 6 to 18 cm. All patients corresponded to tumor stage T3, and 42.86% of patients had lymph node metastases. Surgical excision was the treatment of choice in most cases (85.71%), while a combination of cemiplimab and RP1 was used in some cases due to the ineffectiveness of treatment or contraindications to other therapies. The authors emphasize the importance of early detection and prevention of modifiable risk factors, such as UV radiation, and a multidisciplinary approach to treatment. Other therapies, including immunotherapy, may become increasingly important.
Foaming and Physico-Mechanical Properties of Geopolymer Pastes Manufactured from Post-Metallurgical Recycled Slag
Authors: Sitarz Mateusz , Zdeb Tomasz , Mróz Katarzyna , Hager Izabela , Setlak Kinga
Published: March 2024

This paper presents a research program aimed towards developing a method of producing lightweight, porous geopolymer composites for the construction industry based on industrial wastes. A direct method involving the addition of chemicals is currently most commonly used to produce the porous mineral structure of a geopolymer matrix. This relies on a reaction in a highly alkaline environment of the geopolymer to produce a gas (usually hydrogen or oxygen) that forms vesicles and creates a network of pores. This paper demonstrates the feasibility of producing a slag-based geopolymer paste foamed with aluminum powder, taking into account different parameters of fresh paste production: the mixing duration, its speed and the timing of foaming agent addition. The foaming process of the fresh paste in terms of the volumetric changes and temperature development of the fresh paste during the curing of the material are observed. After hardening, the physical properties (density and porosity) as well as the mechanical parameters (compressive strength and work of damage) are determined for the nine manufactured foamed pastes. Image analysis software was used to assess the porosity distribution of the material across the cross-section of the samples. The results enabled the design of the mixing procedure to be adopted during the manufacture of such composites.
Macroscopic and Microscopic Investigation of Gypsum Slag Cement-Stabilized Recycled Aggregate Base Layers
Authors: Zhou Changdong , Shi Pengcheng , Huang Hao , Shen Junan
Published: March 2024

The purpose of this study is to investigate the macro and micro properties of stabilized recycled aggregate base layers using gypsum slag cement (GSC) and compare them with ordinary Portland cement (OPC). To achieve this, four levels of recycled aggregate content (0%, 50%, 60%, 70%) and three levels of binder materials (3.5%, 4.5%, 5.5%) were designed, where the binding materials included OPC and GSC. When GSC is used as the binding material with 0% recycled content, two scenarios for the ratio of slag to activator are considered: 4:1 and 4:2. For recycled content of 50%, 60%, and 70%, only the 4:1 ratio is considered. The macro-mechanical properties of the composite material were studied through compaction tests, unconfined compressive strength tests, and indirect tensile strength tests. Microscopic properties were investigated through X-ray diffraction (XRD) and scanning electron microscopy (SEM). Macroscopic test results indicate that, at an equal binder content, GSC exhibits a higher moisture content and maximum dry density compared to OPC. Moreover, the unconfined compressive strength and indirect tensile strength of GSC are higher than those of OPC. Microscopic test results reveal that the hydration products of both binding materials are essentially similar; however, under identical curing conditions, the hydration products of GSC are more abundant than those of OPC.
Microwave-Assisted Synthesis of Flower-like MnMoO4 Nanostructures and Their Photocatalytic Performance
Authors: Selvamani Muthamizh , Kesavan Arulvarman , Arulraj Arunachalam , Ramamurthy Praveen C. , Rahaman Mostafizur , Pandiaraj Saravanan , Thiruvengadam Muthu , Sacari Sacari Elisban Juani , Limache Sandoval Elmer Marcial , Viswanathan Mangalaraja Ramalinga
Published: March 2024

This article describes an affordable method for the synthesis of MnMoO4 nanoflowers through the microwave synthesis approach. By manipulating the reaction parameters like solvent, pH, microwave power, and irradiation duration along this pathway, various nanostructures can be acquired. The synthesized nanoflowers were analyzed by using a powder X-ray diffractometer (XRD), field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), and UV–vis diffuse reflectance spectroscopy (UV–DRS) to determine their crystalline nature, morphological and functional group, and optical properties, respectively. X-ray photoelectron spectroscopy (XPS) was performed for the examination of elemental composition and chemical states by qualitative and quantitative analysis. The results of the investigations demonstrated that the MnMoO4 nanostructures with good crystallinity and distinct shape were formed successfully. The synthesized MnMoO4 nanoflowers were tested for their efficiency as a photocatalyst in the degradation studies of methylene blue (MB) as model organic contaminants in an aqueous medium under visible light, which showed their photocatalytic activity with a degradation of 85%. Through the band position calculations using the electronegative value of MnMoO4, the photocatalytic mechanism of the nanostructures was proposed. The results indicated that the effective charge separation, and transfer mechanisms, in addition to the flower-like shape, were responsible for the photocatalytic performance. The stability of the recovered photocatalyst was examined through its recyclability in the degradation of MB. Leveraging MnMoO4’s photocatalytic properties, future studies may focus on scaling up these processes for practical and large-scale environmental remediation.
Study on Properties of Glass-Fiber-Fabric-Reinforced Microwave-Absorbing Composites
Authors: Zhou Zhuohui , Liu Yang , Chen Xi , Wang Zhiyong , Zhao Yan
Published: March 2024

In this paper, the glass-fiber-fabric-reinforced resin-based absorbing composites were prepared, and their microwave-absorbing properties were studied via simulation and experiment. The simulation results show that the absorption bandwidth of the absorbing material can cover the X\C\S band, respectively, at different thicknesses. The minimum reflection loss (RL) of the composite with a thickness of 2.2 mm is −27.4 dB at 5.95 GHz. However, the experiment results are quite different from those of the simulation. The metallographic results indicate that it is the change of the mass fraction of the absorbents in the composites after curing that causes the difference. According to the metallographic results, three shape approximation methods were proposed to calculate the real mass ratio of the absorbents in the composites, namely, parallelogram approximation, bows approximation, and elliptical approximation. Meanwhile, the structural parameter Kf was introduced to optimize the calculation results. The electromagnetic parameters of the material based on the calculation results were measured, and the results show that the simulation results obtained via bow approximation have a better coincidence to the experiment results, and the mass ratio of the absorbent raises by around 9.95%, which lays a foundation for the subsequent design of microwave-absorbing composites.
Study of the Properties and Modification Mechanism of SBS-Modified Asphalt by Dry Process
Authors: Wang Ying , Guo Shaohua , Pei Zhongshi , Zhan Shizuo , Lin Senlin , Ma Kezheng , Lei Junwen , Yi Junyan
Published: March 2024

SBS (styrene-butadiene-styrene block copolymer) is a thermoplastic elastomer with properties most similar to rubber. SBS asphalt modifier is mainly composed of a styrene-butadiene-styrene block copolymer with a certain amount of additives and stabilizers. SBS-modified asphalt binder has always been the most commonly used pavement material both domestically and internationally. However, conventional wet-process SBS-modified asphalt binder requires manufacturers to produce it in advance and transport it to a mixing plant for blending. This has provided an opportunity for unscrupulous businesses to reduce the amount of SBS by adding other substances, allowing inferior asphalt binder to pass inspections undetected. At the same time, conventional wet-process SBS-modified asphalt tends to undergo phase separation and experience a decline in performance as the storage time increases. However, dry-process SBS-modified asphalt can be directly added at the mixing plant, effectively addressing the issues associated with conventional wet-process SBS-modified asphalt. It also helps to reduce environmental pollution to a certain extent. This study investigates the extraction process of dry-process SBS-modified asphalt binder. It clarifies the performance and modification mechanisms of two types of dry-process SBS-modified asphalt binder at different dosages through various testing methods, including basic indicators, rheological properties, infrared spectroscopy, and fluorescence microscopy. The results indicate that due to the incorporation of oil, crosslinker, solubilizer, and other substances into dry-process SBS modifier, there is a small amount of chemical reaction with asphalt in the melting process. The high- and low-temperature properties and fatigue properties of the two dry-process SBS-modified asphalt binders at a 7% dosage are close to wet SBS-modified asphalt binder at a 5% dosage.
Enhancing Fused Deposition Modeling Precision with Serial Communication-Driven Closed-Loop Control and Image Analysis for Fault Diagnosis-Correction
Authors: Behseresht Saeed , Love Allen , Valdez Pastrana Omar Alejandro , Park Young Ho
Published: March 2024

Additive manufacturing (AM) also commonly known as 3D printing is an advanced technique for manufacturing complex three-dimensional (3D) parts by depositing raw material layer by layer. Various sub-categories of additive manufacturing exist including directed energy deposition (DED), powder bed fusion (PBF), and fused deposition modeling (FDM). FDM has gained widespread adoption as a popular method for manufacturing 3D parts, even for heavy-duty industrial applications. However, challenges remain, particularly regarding part quality. Print parameters such as print speed, nozzle temperature, and flow rate can significantly impact the final product’s quality. To address this, implementing a closed-loop quality control system is essential. This system consistently monitors part surface quality during printing and adjusts print parameters upon defect detection. In this study, we propose a simple yet effective image analysis-based closed-loop control system, utilizing serial communication and Python v3.12, a widely accessible software platform. The system’s accuracy and robustness are evaluated, demonstrating its effectiveness in ensuring FDM-printed part quality. Notably, this control system offers superior speed in restoring part quality to normal upon defect detection and is easily implementable on commercially available FDM 3D printers, fostering decentralized quality manufacturing.
Urine Metabolite Profiles after the Consumption of a Low- and a High-Digestible Protein Meal, and Comparison of Urine Normalization Techniques
Authors: Khodorova Nadezda , Calvez Juliane , Pilard Serge , Benoit Simon , Gaudichon Claire , Rutledge Douglas N.
Published: March 2024

In the context of dietary transition toward plant proteins, it is necessary to ensure protein security in populations. It would thus be of interest to identify biomarkers of altered protein digestibility in populations. We examined the association between urinary metabolites and the acute intake of low- or highly digestible protein in healthy volunteers. The urine samples were collected before and 9 h after the ingestion of a meal containing either no protein, zein (low-digestible) or whey protein isolate (highly digestible). The liquid chromatography–high resolution mass spectrometry metabolomics approach was used for the profiling of the urinary metabolites. For the standardization of metabolomics data sets, osmolality-based, standard normal variates (SNV) and probabilistic quotient normalization (PQN) techniques were used. The ANOVA-based factorial method, AComDim_ICA, was used for chemometrics analysis. The osmolality adjustment has a beneficial effect and the subsequent mathematical normalization improves the chemometric analysis further. Some changes in the urinary metabolomes were observed 9 h after the meal in the three groups. However, there was no difference in the urine metabolome between groups. No biomarker of protein digestibility can be identified after the ingestion of a single meal, even when marked differences in the digestion efficiency of protein have been observed.
Studying Salt-Induced Shifts in Gene Expression Patterns of Glucosinolate Transporters and Glucosinolate Accumulation in Two Contrasting Brassica Species
Authors: Fatima Samia , Khan Muhammad Omar , Iqbal Nadia , Iqbal Muhammad Mudassar , Qamar Huma , Imtiaz Muhammad , Hundleby Penny , Wei Zhengyi , Ahmad Niaz
Published: March 2024

Brassica crops are well known for the accumulation of glucosinolates—secondary metabolites crucial for plants’ adaptation to various stresses. Glucosinolates also functioning as defence compounds pose challenges to food quality due to their goitrogenic properties. Their disruption leaves plants susceptible to insect pests and diseases. Hence, a targeted reduction in seed glucosinolate content is of paramount importance to increase food acceptance. GLUCOSINOLATE TRANSPORTERS (GTRs) present a promising avenue for selectively reducing glucosinolate concentrations in seeds while preserving biosynthesis elsewhere. In this study, 54 putative GTR protein sequences found in Brassica were retrieved, employing Arabidopsis GTR1 and GTR2 templates. Comprehensive bioinformatics analyses, encompassing gene structure organization, domain analysis, motif assessments, promoter analysis, and cis-regulatory elements, affirmed the existence of transporter domains and stress-related regulatory elements. Phylogenetic analysis revealed patterns of conservation and divergence across species. Glucosinolates have been shown to increase under stress conditions, indicating a potential role in stress response. To elucidate the role of GTRs in glucosinolate transportation under NaCl stress in two distinct Brassica species, B. juncea and B. napus, plants were subjected to 0, 100, or 200 mM NaCl. Based on the literature, key GTR genes were chosen and their expression across various plant parts was assessed. Both species displayed divergent trends in their biochemical profiles as well as glucosinolate contents under elevated salt stress conditions. Statistical modelling identified significant contributors to glucosinolate variations, guiding the development of targeted breeding strategies for low-glucosinolate varieties. Notably, GTR2A2 exhibited pronounced expressions in stems, contributing approximately 52% to glucosinolate content variance, while GTR2B1/C2 displayed significant expression in flowers. Additionally, GTR2A1 and GTR1A2/B1 demonstrated noteworthy expression in roots. This study enhances our understanding of glucosinolate regulation under stress conditions, offering avenues to improve Brassica crop quality and resilience.
Interaction of Negative Bias Instability and Self-Heating Effect on Threshold Voltage and SRAM (Static Random-Access Memory) Stability of Nanosheet Field-Effect Transistors
Authors: Li Xiaoming , Shao Yali , Wang Yunqi , Liu Fang , Kuang Fengyu , Zhuang Yiqi , Li Cong
Published: March 2024

In this paper, we investigate the effects of negative bias instability (NBTI) and self-heating effect (SHE) on threshold voltage in NSFETs. To explore accurately the interaction between SHE and NBTI, we established an NBTI simulation framework based on trap microdynamics and considered the influence of the self-heating effect. The results show that NBTI weakens the SHE effect, while SHE exacerbates the NBTI effect. Since the width of the nanosheet in NSFET has a significant control effect on the electric field distribution, we also studied the effect of the width of the nanosheet on the NBTI and self-heating effect. The results show that increasing the width of the nanosheet will reduce the NBTI effect but will enhance the SHE effect. In addition, we extended our research to the SRAM cell circuit, and the results show that the NBTI effect will reduce the static noise margin (SNM) of the SRAM cell, and the NBTI effect affected by self-heating will make the SNM decrease more significantly. In addition, our research results also indicate that increasing the nanosheet width can help slow down the NBTI effect and the negative impact of NBTI on SRAM performance affected by the self-heating effect.
Dietary Fructose and Sodium Consumed during Early Mid-Life Are Associated with Hypertensive End-Organ Damage by Late Mid-Life in the CARDIA Cohort
Authors: Komnenov Dragana , Al-Hadidi Mohammad , Ali Hamza , Al-Jamal Malik , Salami Kassim , Shelbaya Samy , Tayeb Kareem , Domin Daniel , Elhamzawy Rana
Published: March 2024

We aimed to investigate how dietary fructose and sodium impact blood pressure and risk of hypertensive target organ damage 10 years later. Data from n = 3116 individuals were obtained from the Coronary Artery Risk Development in Young Adults (CARDIA) study. Four groups were identified based on the four possible combinations of the lower and upper 50th percentile for sodium (in mg) and fructose (expressed as percent of total daily calories). Differences among groups were ascertained and logistic regression analyses were used to assess the risk of hypertensive target organ damage (diastolic dysfunction, coronary calcification and albuminuria). Individuals in the low-fructose + low-sodium group were found to have lower SBP compared to those in the low-fructose + high-sodium and high-fructose + high-sodium groups (p < 0.05). The highest risk for hypertensive target organ damage was found for albuminuria only in the high-fructose + high-sodium group (OR = 3.328, p = 0.006) while female sex was protective across all groups against coronary calcification. Our findings highlight that sodium alone may not be the culprit for hypertension and hypertensive target organ damage, but rather when combined with an increased intake of dietary fructose, especially in middle-aged individuals.
Characterizing Food Policy Councils’ Network Partnerships and COVID-19 Responses
Authors: Mui Yeeli , Adam Atif , Santo Raychel , Bassarab Karen , Wolfson Julia A. , Palmer Anne
Published: March 2024

The COVID-19 pandemic pushed millions of Americans into food insecurity. Food policy councils (FPCs) across the country played a vital role in organizing coordinated food responses across multiple sectors. We used a social network analysis (SNA) approach to investigate: (1) the network of partnering organizations and agencies within FPCs; (2) how the characteristics of FPCs’ network partnerships (i.e., degree, coreness, and density) related to programmatic, policy, and advocacy actions in response to the pandemic; and (3) how FPCs’ use of a racial or social equity framework shifted their network partnerships and responses. Local government agencies and food supply chain actors were core in FPCs’ network partnerships, while public utilities, correctional facilities, social justice groups, and others were non-core partners. Network density was more likely to be associated with any action by FPCs, and it was especially pronounced for advocacy actions taken by FPCs; trends were similar among FPCs that reported using a racial or social equity framework. The findings begin to uncover core actors in FPCs’ partnerships and opportunities to establish new partnerships, particularly with social justice groups. The results also suggest that network density (interconnectedness) may be more important than other network characteristics when responding to food-related needs.
Cuban Policosanol (Raydel®) Exerts Higher Antioxidant and Anti-Glycation Activities than Chinese Policosanol (BOC Sciences) in Reconstituted High-Density Lipoproteins: In Vivo Anti-Inflammatory Activities in Zebrafish and Its Embryos
Authors: Cho Kyung-Hyun , Kim Ji-Eun , Lee Myeong-Sung , Bahuguna Ashutosh
Published: March 2024

The present study compares sugarcane-wax purified policosanols sourced from Cuba (Raydel®) and China (BOC Sciences) and utilized following the synthesis of reconstituted high-density lipoproteins (rHDL). The two policosanols exhibited distinctly different ingredient ratios of long-chain aliphatic alcohols, particularly 1-octacosanol (C28) and 1-tetratriacotanol (C34). After synthesizing rHDL with apolipoprotein A-I (apoA-I), the two policosanols bound well with phospholipid and apoA-I to form the discoidal rHDL. Notably, rHDL-1, containing Cuban policosanol, displayed the largest particle diameter at approximately 78 ± 3 nm. In contrast, both control rHDL (rHDL-0) and rHDL containing Chinese policosanol (rHDL-2) exhibited smaller particles, with diameters of approximately 58 ± 3 nm and 61 ± 2 nm, respectively. Furthermore, rHDL-1 demonstrated enhanced anti-glycation activity, safeguarding apoA-I from degradation within HDL, and displayed the antioxidant ability to inhibit LDL oxidation. A microinjection of each rHDL into zebrafish embryos in the presence of carboxymethyllysine (CML) revealed rHDL-1 to have the strongest antioxidant activity with the highest embryo survivability and normal developmental morphology. Dermal application to recover the wound revealed rHDL-1 to have the highest wound-healing activity (75%) and survivability (92%) in the cutaneous wound area in the presence of CML. In adult zebrafish, injecting CML (250 μg) caused acute death and hyperinflammation, marked by heightened neutrophil infiltration and interleukin (IL)-6 production in liver. However, co-administering rHDL-1 notably increased survival (85%) and exhibited strong anti-inflammatory properties, reducing IL-6 production while improving the blood lipid profile. However, a co-injection of rHDL-2 resulted in the lowest survivability (47%) with more hepatic inflammation. In conclusion, Cuban policosanol (Raydel®) has more desirable properties for the in vitro synthesis of rHDL with stronger anti-glycation and antioxidant activities than those of Chinese policosanol (BOC Sciences). Moreover, Raydel-policosanol-integrated rHDL demonstrates a noteworthy effect on accelerated wound healing and robust anti-inflammatory properties, leading to increased survivability in zebrafish embryos and adults by effectively suppressing CML-induced hyperinflammation.
Nucleoside Reverse Transcriptase Inhibitor Exposure Is Associated with Lower Alzheimer’s Disease Risk: A Retrospective Cohort Proof-of-Concept Study
Authors: Chow Tiffany W. , Raupp Mark , Reynolds Matthew W. , Li Siying , Kaeser Gwendolyn E. , Chun Jerold
Published: March 2024

Brain somatic gene recombination (SGR) and the endogenous reverse transcriptases (RTs) that produce it have been implicated in the etiology of Alzheimer’s disease (AD), suggesting RT inhibitors as novel prophylactics or therapeutics. This retrospective, proof-of-concept study evaluated the incidence of AD in people with human immunodeficiency virus (HIV) with or without exposure to nucleoside RT inhibitors (NRTIs) using de-identified medical claims data. Eligible participants were aged ≥60 years, without pre-existing AD diagnoses, and pursued medical services in the United States from October 2015 to September 2016. Cohorts 1 (N = 46,218) and 2 (N = 32,923) had HIV. Cohort 1 had prescription claims for at least one NRTI within the exposure period; Cohort 2 did not. Cohort 3 (N = 150,819) had medical claims for the common cold without evidence of HIV or antiretroviral therapy. The cumulative incidence of new AD cases over the ensuing 2.75-year observation period was lowest in patients with NRTI exposure and highest in controls. Age- and sex-adjusted hazard ratios showed a significantly decreased risk for AD in Cohort 1 compared with Cohorts 2 (HR 0.88, p < 0.05) and 3 (HR 0.84, p < 0.05). Sub-grouping identified a decreased AD risk in patients with NRTI exposure but without protease inhibitor (PI) exposure. Prospective clinical trials and the development of next-generation agents targeting brain RTs are warranted.
In Vivo Applications of Dendrimers: A Step toward the Future of Nanoparticle-Mediated Therapeutics
Authors: Sztandera Krzysztof , Rodríguez-García José Luis , Ceña Valentín
Published: March 2024

Over the last few years, the development of nanotechnology has allowed for the synthesis of many different nanostructures with controlled sizes, shapes, and chemical properties, with dendrimers being the best-characterized of them. In this review, we present a succinct view of the structure and the synthetic procedures used for dendrimer synthesis, as well as the cellular uptake mechanisms used by these nanoparticles to gain access to the cell. In addition, the manuscript reviews the reported in vivo applications of dendrimers as drug carriers for drugs used in the treatment of cancer, neurodegenerative diseases, infections, and ocular diseases. The dendrimer-based formulations that have reached different phases of clinical trials, including safety and pharmacokinetic studies, or as delivery agents for therapeutic compounds are also presented. The continuous development of nanotechnology which makes it possible to produce increasingly sophisticated and complex dendrimers indicates that this fascinating family of nanoparticles has a wide potential in the pharmaceutical industry, especially for applications in drug delivery systems, and that the number of dendrimer-based compounds entering clinical trials will markedly increase during the coming years.
An Evaluation of the Effectivity of the Green Leaves Biostimulant on Lettuce Growth, Nutritional Quality, and Mineral Element Efficiencies under Optimal Growth Conditions
Authors: Atero-Calvo Santiago , Izquierdo-Ramos María José , García-Huertas Carmen , Rodríguez-Alcántara Miguel , Navarro-Morillo Iván , Navarro-León Eloy
Published: March 2024

The use of biostimulants is becoming a useful tool for increasing crop productivity while enhancing nutritional quality. However, new studies are necessary to confirm that the joint application of different types of biostimulants, together with bioactive compounds, is effective and not harmful to plants. This study examined the impact of applying the biostimulant Green Leaves, comprising Macrocystis algae extract and containing a mixture of amino acids, corn steep liquor extract, calcium, and the bioactive compound glycine betaine. The effect of applying two different doses (3 and 5 mL L−1) of this biostimulant was evaluated on lettuce plants, and growth and quality parameters were analyzed along with photosynthetic efficiency, nutritional status, and nutrient efficiency parameters. The application of Green Leaves improved plant weight (25%) and leaf area and enhanced the photosynthetic rate, the accumulation of soluble sugars and proteins, and the agronomic efficiency of all essential nutrients. The 3 mL L−1 dose improved the nutritional quality of lettuce plants, improving the concentration of phenolic compounds and ascorbate and the antioxidant capacity and reducing NO3− accumulation. The 5 mL L−1 dose improved the absorption of most nutrients, especially N, which reduced the need for fertilizers, thus reducing costs and environmental impact. In short, the Green Leaves product has been identified as a useful product for obtaining higher yield and better quality.
“Purplish Blue” or “Greenish Grey”? Indigo Qualities and Extraction Yields from Six Species
Authors: Hartl Anna , Polleichtner Andrea , Novak Johannes
Published: March 2024

Indigo quality is determined by its indigotin content. Another quality indicator is colour. For an evaluation of species, indigo samples from Indigofera tinctoria, Indigofera suffruticosa, Indigofera arrecta, Persicaria tinctoria, Strobilanthes cusia and Wrightia laevis cultivated in Austria and China were visually classified and analysed spectrophotometrically and using a L*a*b* measuring device. In addition to a standardised hot-extraction method without lime, some samples were extracted simulating traditional methods at ambient temperatures using lime. The highest indigotin contents were achieved with Indigofera arrecta (55%, Austria) and Strobilanthes cusia (56%, China). There were no statistically significant differences between the indigo extraction yields of the species cultivated in Austria, but Indigofera arrecta and Persicaria tinctoria had statistically significantly higher indigotin extraction yields than Indigofera tinctoria and Indigofera suffruticosa. From the species extracted in China, Strobilanthes cusia showed higher values in all parameters than Indigofera tinctoria, Indigofera suffruticosa and Wrightia laevis. Compared with the standardised method, the method simulating local practice yielded more indigo but had a lower indigotin content; the indigotin extraction yields did not differ greatly. L*a*b* values enabled precise estimations of the indigotin content, making it an interesting option for quality control, as inexpensive, easy-to-handle L*a*b* measuring instruments have become available.
Exploring Intraspecific Trait Variation in a Xerophytic Moss Species Indusiella thianschanica (Ptychomitriaceae) across Environmental Gradients on the Tibetan Plateau
Authors: Song Xiaotong , Gu Jiqi , Ye Yanhui , Wang Mengzhen , Wang Ruihong , Ma Heping , Shao Xiaoming
Published: March 2024

Investigating intraspecific trait variability is crucial for understanding plant adaptation to various environments, yet research on lithophytic mosses in extreme environments remains scarce. This study focuses on Indusiella thianschanica Broth. Hal., a unique lithophytic moss species in the extreme environments of the Tibetan Plateau, aiming to uncover its adaptation and response mechanisms to environmental changes. Specimens were collected from 26 sites across elevations ranging from 3642 m to 5528 m, and the relationships between 23 morphological traits and 15 environmental factors were analyzed. Results indicated that coefficients of variation (CV) ranged from 5.91% to 36.11%, with gametophyte height (GH) and basal cell transverse wall thickness (STW) showing the highest and lowest variations, respectively. Temperature, elevation, and potential evapo-transpiration (PET) emerged as primary environmental drivers. Leaf traits, especially those of the leaf sheath, exhibited a more pronounced response to the environment. The traits exhibited apparent covariation in response to environmental challenges and indicated flexible adaptive strategies. This study revealed the adaptation and response patterns of different morphological traits of I. thianschanica to environmental changes on the Tibetan Plateau, emphasizing the significant effect of temperature on trait variation. Our findings deepen the understanding of the ecology and adaptive strategies of lithophytic mosses.
The Potential of Plants to Absorb Xenobiotics
Authors: Capozzi Fiore , Spagnuolo Valeria
Published: March 2024

Environmental pollution is a pressing problem that endangers our biosphere [...]
The Effect of Different Factors on Poly(lactic-co-glycolic acid) Nanoparticle Properties and Drug Release Behaviors When Co-Loaded with Hydrophilic and Hydrophobic Drugs
Authors: Wang Lianguo , Wang Pei , Liu Yifan , Mustafa Mahayyudin Muhammad Atae , Li Rong , Zhang Weilun , Zhan Yilan , Li Zhihua
Published: March 2024

Poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) are versatile drug nanocarriers with a wide spectrum of applications owing to their extensive advantages, including biodegradability, non-toxic side effects, and low immunogenicity. Among the numerous nanoparticle preparation methods available for PLGA NPs (the hydrophobic polymer), one of the most extensively utilized preparations is the sonicated-emulsified solvent evaporation method, owing to its simplicity, speed, convenience, and cost-effectiveness. Nevertheless, several factors can influence the outcomes, such as the types of concentration of the surfactants and organic solvents, as well as the volume of the aqueous phase. The objective of this article is to explore the influence of these factors on the properties of PLGA NPs and their drug release behavior following encapsulation. Herein, PLGA NPs were fabricated using bovine serum albumin (BSA) as a surfactant to investigate the impact of influencing factors, including different water-soluble organic solvents such as propylene carbonate (PC), ethyl acetate (PA), and dichloromethane (DCM). Notably, the size of PLGA NPs was smaller in the EA group compared to that in the DCM group. Moreover, PLGA NPs showed excellent stability, ascribed to the presence of the BSA surfactant. Furthermore, PLGA NPs were co-loaded with varying concentrations of hydrophilic drugs (doxorubicin hydrochloride) and hydrophobic drugs (celecoxib), and exhibited pH-sensitive drug release behavior in PBS with pH 7.4 and pH 5.5.
Environment-Friendly Preparation and Characterization of Multilayered Conductive PVP/Col/CS Composite Doped with Nanoparticles as Potential Nerve Guide Conduits
Authors: Sierakowska-Byczek Aleksandra , Radwan-Pragłowska Julia , Janus Łukasz , Galek Tomasz , Łysiak Karol , Tupaj Mirosław , Bogdał Dariusz
Published: March 2024

Tissue engineering constitutes the most promising method of severe peripheral nerve injuries treatment and is considered as an alternative to autografts. To provide appropriate conditions during recovery special biomaterials called nerve guide conduits are required. An ideal candidate for this purpose should not only be biocompatible and protect newly forming tissue but also promote the recovery process. In this article a novel, multilayered biomaterial based on polyvinylpyrrolidone, collagen and chitosan of gradient structure modified with conductive nanoparticles is presented. Products were obtained by the combination of electrospinning and electrospraying techniques. Nerve guide conduits were subjected to FT-IR analysis, morphology and elemental composition study using SEM/EDS as well as biodegradation. Furthermore, their effect on 1321N1 human cell line was investigated by long-term cell culture. Lack of cytotoxicity was confirmed by XTT assay and morphology study. Obtained results confirmed a high potential of newly developed biomaterials in the field of nerve tissue regeneration with a special focus on injured nerves recovery.
Comparative Analysis of the Mechanical Properties and Biocompatibility between CAD/CAM and Conventional Polymers Applied in Prosthetic Dentistry
Authors: Chuchulska Bozhana , Dimitrova Mariya , Vlahova Angelina , Hristov Ilian , Tomova Zlatina , Kazakova Rada
Published: March 2024

Modern media often portray CAD/CAM technology as widely utilized in the fabrication of dental prosthetics. This study presents a comparative analysis of the mechanical properties and biocompatibility of CAD/CAM (Computer-Aided Design/Computer-Aided Manufacturing) polymers and conventional polymers commonly utilized in prosthetic dentistry. With the increasing adoption of CAD/CAM technology in dental laboratories and practices, understanding the differences in material properties is crucial for informed decision-making in prosthodontic treatment planning. Through a narrative review of the literature and empirical data, this study evaluates the mechanical strength, durability, esthetics, and biocompatibility of CAD/CAM polymers in comparison to traditional polymers. Furthermore, it examines the implications of these findings on the clinical outcomes and long-term success of prosthetic restorations. The results provide valuable insights into the advantages and limitations of CAD/CAM polymers, informing clinicians and researchers about their suitability for various dental prosthetic applications. This study underscores the considerable advantages of CAD/CAM polymers over conventional ones in terms of mechanical properties, biocompatibility, and esthetics for prosthetic dentistry. CAD/CAM technology offers improved mechanical strength and durability, potentially enhancing the long-term performance of dental prosthetics, while the biocompatibility of these polymers makes them suitable for a broad patient demographic, reducing the risk of adverse reactions. The practical implications of these findings for dental technicians and dentists are significant, as understanding these material differences enables tailored treatment planning to meet individual patient needs and preferences. Integration of CAD/CAM technology into dental practices can lead to more predictable outcomes and heightened patient satisfaction with prosthetic restorations.
Narrative Disjunction, Artful Occlusion, and Cryptic Commentary in Joshua 1–12
Authors: Baker Robin
Published: March 2024

The book of Joshua is a book-length crux interpretum. Its cultivation of two concurrent narratives that contradict one another has fascinated commentators since antiquity. This is only one of its hermeneutically challenging features. Most modern commentaries attribute these features to an uneven process of redaction. Focusing on chapters 1–12, this essay argues that incongruity, ambiguity, and disjunction are essential elements of a rhetorical strategy in which form and content are imaginatively correlated, and that the text contains many cryptic clues that are necessary to elucidate its meaning. It contends that Joshua is the product of a bold literary innovation that is motivated by a sustained and uncompromising determination to unsettle facile assumptions about YHWH and Israel’s history. It concludes that the book challenges us to re-evaluate not only the answers we thought it gave, but even the questions we ask of it.
Seismic Data Reconstruction Using a Phase-Shift-Plus-Interpolation-Based Apex-Shifted Hyperbolic Radon Transform
Authors: Wang Yue , Gong Xiangbo , Hu Bin
Published: March 2024

The apex-shifted hyperbolic Radon transform (ASHRT) based on the Stolt-stretch operator can be implemented in the frequency domain, which accelerates the computation efficiency of ASHRT. However, the Stolt-stretch operator has limitations when it comes to velocity variations. Therefore, this paper introduces a new ASHRT approach based on post-stack phase shift plus interpolation (PSPI) imaging and modeling operators. This new approach is designed to better adapt to changes in medium velocity and enhance the quality of data reconstruction. When combining this novel transformation with sparsity constraints for model testing and real data applications, the experimental results indicate that it is an effective data reconstruction tool, with superior data reconstruction results compared to traditional ASHRT based on the Stolt-stretch operator.
Trust in Anonymous News? How Users Navigate Political News Channels on Russian Telegram
Authors: Litvinenko Anna , Smoliarova Anna
Published: March 2024

The paper explores the phenomenon of anonymous news channels on Russian Telegram, which have become increasingly popular in recent years. Drawing on 25 self-confrontation interviews, we answer the following questions: Do users trust anonymous news? If not, why do they keep using this information source? How does a restrictive socio-political context influence users’ trust in alternative news sources? Our results show that, in Russia, the concept of trust is linked to the normative democratic understanding of journalistic functions. At the same time, many users believe that trust in media is not at all necessary and develop individual strategies to navigate a “chaos of narratives”. The paper discusses Telegram’s role in shaping trust or distrust in news.