bluuebunny/crossref_metadata_2025 huggingface dataset:
Addition of Probiotics to Anti-Obesity Therapy by Percutaneous Electrical Stimulation of Dermatome T6. A Pilot Study
Authors: Lorenzo Oscar , Crespo-Yanguas Marta , Hang Tianyu , Lumpuy-Castillo Jairo , Hernández Artur M. , Llavero Carolina , García-Alonso MLuisa , Ruiz-Tovar Jaime
Published: October 2020

Obesity is becoming a pandemic and percutaneous electrical stimulation (PENS) of dermatome T6 has been demonstrated to reduce stomach motility and appetite, allowing greater weight loss than isolated hypocaloric diets. However, modulation of intestinal microbiota could improve this effect and control cardiovascular risk factors. Our objective was to test whether addition of probiotics could improve weight loss and cardiovascular risk factors in obese subjects after PENS and a hypocaloric diet. A pilot prospective study was performed in patients (n = 20) with a body mass index (BMI) > 30 kg/m2. Half of them underwent ten weeks of PENS in conjunction with a hypocaloric diet (PENS-Diet), and the other half was treated with a PENS-Diet plus multistrain probiotics (L. plantarum LP115, B. brevis B3, and L. acidophilus LA14) administration. Fecal samples were obtained before and after interventions. The weight loss and changes in blood pressure, glycemic and lipid profile, and in gut microbiota were investigated. Weight loss was significantly higher (16.2 vs. 11.1 kg, p = 0.022), whereas glycated hemoglobin and triglycerides were lower (−0.46 vs. −0.05%, p = 0.032, and −47.0 vs. −8.5 mg/dL, p = 0.002, respectively) in patients receiving PENS-Diet + probiotics compared with those with a PENS-Diet. Moreover, an enrichment of anti-obesogenic bacteria, including Bifidobacterium spp, Akkermansia spp, Prevotella spp, and the attenuation of the Firmicutes/Bacteroidetes ratio were noted in fecal samples after probiotics administration. In obese patients, the addition of probiotics to a PENS intervention under a hypocaloric diet could further improve weight loss and glycemic and lipid profile in parallel to the amelioration of gut dysbiosis.
Association between Non-Restorative Sleep and Quality of Life in Chinese Adolescents
Authors: Chen Ningjing , Fong Daniel Yee Tak , Li Sha , Wong Janet Yuen Ha
Published: October 2020

To examine non-restorative sleep and its impact on quality of life (QOL) in Chinese adolescents, this cross-sectional study included 2827 students aged 12–20 who were selected from 15 secondary schools in Hong Kong, China. Non-restorative sleep was assessed by a single item, rated on a 0–10 scale: “To what extent did you feel refreshed upon awakening over the past month?”. QOL was evaluated by the World Health Organization Quality of Life Measure—Abbreviated Version Hong Kong Chinese Version. Univariable and multivariable linear mixed-effects regressions were conducted to examine the influence of non-restorative sleep (NRS) on QOL. In the present sample, the mean overall QOL was 13.83, and the extent of feeling refreshed upon awakening was 4.75 on average. In multivariable linear mixed-effects regression, one unit feeling less refreshed upon awakening was associated with 0.37 units of poorer overall QOL after adjusting for age, gender, medical conditions, parental education and occupation, weekly hours of aerobic exercises, smoking and drinking habits. Additionally, adolescents with a significantly poorer overall QOL were more likely to be older, have medical problems, have parents with an educational level of primary school or below, have an unemployed father, engage in less weekly aerobic exercise, and be current smokers or former drinkers. Adolescents who experienced non-restorative sleep had a poorer QOL. Future studies are needed to alleviate non-restorative sleep to improve health outcomes in adolescents.
Association of Periodontitis with Atherosclerotic Cardiovascular Diseases: A Nationwide Population-based Retrospective Matched Cohort Study
Authors: Byon Min-Ji , Kim Se-Yeon , Kim Ji-Soo , Kim Han-Na , Kim Jin-Bom
Published: October 2020

We investigated the association between periodontitis and atherosclerotic cardiovascular disease (ACVD) development using the National Health Insurance Service—National Sample Cohort 2.0 (NHIS-NSC2) database, which contains data for approximately 1 million nationally representative random participants. We selected 52,425 participants aged 20+ years and diagnosed with periodontitis from January to December 2003 and used propensity score matching to select an equivalent number of participants who were never diagnosed with periodontitis in the period covered by the NHIS-NSC2 database (2002–2015). The propensity scores were based on sex, age group, type of national health insurance, household income, diabetes status, and hypertension status and were used for 1:1 matching of individuals with similar propensities. A total of 104,850 participants were selected for the study. A multivariable Cox proportional hazard regression model was used to investigate the risk of ACVD development due to periodontitis from 2003 to 2015 after adjusting for sex, age, type of national health insurance, household income, hypertension status, and diabetes status. Participants with periodontitis had a higher risk of ACVD (adjusted hazard ratio: 1.09, 95% confidence interval: 1.05–1.13) than those without periodontitis. Thus, periodontitis can increase the risk of ACVD, and prevention of periodontitis may help reduce the risk of cardiovascular disease.
Reply to “Comment on Fitria et al. ‘Environmental and Occupational Risk Factors Associated with Chronic Kidney Disease of Unknown Etiology in West Javanese Rice Farmers, Indonesia’ Int. J. Environ. Res. Public Health, 2020, 17, 4521”
Authors: Fitria Laila , Prihartono Nurhayati Adnan , Ramdhan Doni Hikmat , Woskie Susan
Published: October 2020

Examining the WBGT (Wet Bulb Globe Temperature) profiles in the two study locations was intended to show temperature differences during the week of the study. Information obtained from the weather stations that provide contextual long-term information on heat and humidity also showed temperature differences. The average measured temperature and humidity in the past year from each of these weather stations show an average heat index of 22 °C in Bogor and an average heat index of 32 °C in Karawang. Interpretation of the chronic kidney disease (CKD) and chronic kidney disease of unknown etiology (CKDu) findings was more complicated because we also found that farmers in our two locations reported differences in the use of mechanization in their farming, presumably impacting their workloads.
How the Lagged and Accumulated Effects of Stress, Coping, and Tasks Affect Mood and Fatigue during Nurses’ Shifts
Authors: Martínez-Zaragoza Fermín , Fernández-Castro Jordi , Benavides-Gil Gemma , García-Sierra Rosa
Published: October 2020

Nurses experience significant stress and emotional exhaustion, leading to burnout and fatigue. This study assessed how the nurses’ mood and fatigue evolves during their shifts, and the temporal factors that influence these phenomena. Performing a two-level design with repeated measures with moments nested into a person level, a random sample of 96 nurses was recruited. The ecological momentary assessment of demand, control, effort, reward, coping, and nursing tasks were measured in order to predict mood and fatigue, studying their current, lagged, and accumulated effects. The results show that: (1) Mood appeared to be explained by effort, by the negative lagged effect of reward, and by the accumulated effort, each following a quadratic trend, and it was influenced by previously executing a direct care task. By contrast, fatigue was explained by the current and lagged effect of effort, by the lagged effect of reward, and by the accumulated effort, again following quadratic trends. (2) Mood was also explained by problem-focused and emotion-focused coping strategies, indicative of negative mood, and by support-seeking and refusal coping strategies. (3) Fatigue was also associated with direct care and the prior effect of documentation and communication tasks. We can conclude that mood and fatigue do not depend on a single factor, such as workload, but rather on the evolution and distribution of the nursing tasks, as well as on the stress during a shift and how it is handled. The evening and night shifts seem to provoke more fatigue than the other work shifts when approaching the last third of the shift. These data show the need to plan the tasks within a shift to avoid unfinished or delayed care during the shift, and to minimize accumulated negative effects.
Nonenzymatic Spontaneous Oxidative Transformation of 5,6-Dihydroxyindole
Authors: Sugumaran Manickam , Evans Jason , Ito Shosuke , Wakamatsu Kazumasa
Published: October 2020

Melanin is an important phenolic skin pigment found throughout the animal kingdom. Tyrosine and its hydroxylated product dopa provide the starting material for melanin biosynthesis in all animals. Through a set of well-established reactions, they are converted to 5,6-dihydroxyindole (DHI) and DHI-2-carboxylic acid (DHICA). Oxidative polymerization of these two indoles produces the brown to black eumelanin pigment. The steps associated with these transformations are complicated by the extreme instability of the starting materials and the transient and highly reactive nature of the intermediates. We have used mass spectral studies to explore the nonenzymatic mechanism of oxidative transformation of DHI in water. Our results indicate the facile production of not only dimeric and trimeric products but also higher oligomeric forms of DHI upon exposure to air in solution, even under nonenzymatic conditions. Such instantaneous polymerization of DHI avoids toxicity to self-matter and ensures the much-needed deposition of melanin at (a) the wound site and (b) the infection site in arthropods. The rapid deposition of DHI melanin is advantageous for arthropods given their open circulatory system; the process limits blood loss during wounding and prevents the spread of parasites by encapsulating them in melanin, limiting the damage.
MicroRNA Expression in the Aqueous Humor of Patients with Diabetic Macular Edema
Authors: Grieco Giuseppina Emanuela , Sebastiani Guido , Eandi Chiara Maria , Neri Giovanni , Nigi Laura , Brusco Noemi , D'Aurizio Romina , Posarelli Matteo , Bacci Tommaso , De Benedetto Elena , Fruschelli Mario , Orlandini Maurizio , Galvagni Federico , Dotta Francesco , Tosi Gian Marco
Published: October 2020

We identified and compared secreted microRNA (miRNA) expression in aqueous humor (AH) and plasma samples among patients with: type 2 diabetes mellitus (T2D) complicated by non-proliferative diabetic retinopathy (DR) associated with diabetic macular edema (DME) (DME group: 12 patients); T2D patients without DR (D group: 8 patients); and non-diabetic patients (CTR group: 10 patients). Individual patient AH samples from five subjects in each group were profiled on TaqMan Low Density MicroRNA Array Cards. Differentially expressed miRNAs identified from profiling were then validated in single assay for all subjects. The miRNAs validated in AH were then evaluated in single assay in plasma. Gene Ontology (GO) analysis was conducted. From AH profiling, 119 mature miRNAs were detected: 86 in the DME group, 113 in the D group and 107 in the CTR group. miRNA underexpression in the DME group was confirmed in single assay for let-7c-5p, miR-200b-3p, miR-199a-3p and miR-365-3p. Of these four, miR-199a-3p and miR-365-3p were downregulated also in the plasma of the DME group. GO highlighted 54 validated target genes of miR-199a-3p, miR-200b-3p and miR-365-3p potentially implied in DME pathogenesis. Although more studies are needed, miR-200b-3p, let-7c-5p, miR-365-3p and miR-199a-3p represent interesting molecules in the study of DME pathogenesis.
Neuroprotective Effect of Curcumin on the Nigrostriatal Pathway in a 6-Hydroxydopmine-Induced Rat Model of Parkinson’s Disease is Mediated by α7-Nicotinic Receptors
Authors: El Nebrisi Eslam , Javed Hayate , Ojha Shreesh K , Oz Murat , Shehab Safa
Published: October 2020

Parkinson’s disease (PD) is a common neurodegenerative disorder, characterized by selective degeneration of dopaminergic nigrostriatal neurons. Most of the existing pharmacological approaches in PD consider replenishing striatal dopamine. It has been reported that activation of the cholinergic system has neuroprotective effects on dopaminergic neurons, and human α7-nicotinic acetylcholine receptor (α7-nAChR) stimulation may offer a potential therapeutic approach in PD. Our recent in-vitro studies demonstrated that curcumin causes significant potentiation of the function of α7-nAChRs expressed in Xenopus oocytes. In this study, we conducted in vivo experiments to assess the role of the α7-nAChR on the protective effects of curcumin in an animal model of PD. Intra-striatal injection of 6-hydroxydopmine (6-OHDA) was used to induce Parkinsonism in rats. Our results demonstrated that intragastric curcumin treatment (200 mg/kg) significantly improved the abnormal motor behavior and offered neuroprotection against the reduction of dopaminergic neurons, as determined by tyrosine hydroxylase (TH) immunoreactivity in the substantia nigra and caudoputamen. The intraperitoneal administration of the α7-nAChR-selective antagonist methyllycaconitine (1 µg/kg) reversed the neuroprotective effects of curcumin in terms of both animal behavior and TH immunoreactivity. In conclusion, this study demonstrates that curcumin has a neuroprotective effect in a 6-hydroxydopmine (6-OHDA) rat model of PD via an α7-nAChR-mediated mechanism.
Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way
Authors: Abuobeid Roubi , Herrera-Marcos Luis , Navarro María A. , Arnal Carmen , Martínez-Beamonte Roberto , Surra Joaquín , Osada Jesús
Published: October 2020

Erythrodiol is a terpenic compound found in a large number of plants. To test the hypotheses that its long-term administration may influence hepatic transcriptome and this could be influenced by the presence of APOA1-containing high-density lipoproteins (HDL), Western diets containing 0.01% of erythrodiol (10 mg/kg dose) were provided to Apoe- and Apoa1-deficient mice. Hepatic RNA-sequencing was carried out in male Apoe-deficient mice fed purified Western diets differing in the erythrodiol content. The administration of this compound significantly up- regulated 68 and down-regulated 124 genes at the level of 2-fold change. These genes belonged to detoxification processes, protein metabolism and nucleic acid related metabolites. Gene expression changes of 21 selected transcripts were verified by RT-qPCR. Ccl19-ps2, Cyp2b10, Rbm14-rbm4, Sec61g, Tmem81, Prtn3, Amy2a5, Cyp2b9 and Mup1 showed significant changes by erythrodiol administration. When Cyp2b10, Dmbt1, Cyp2b13, Prtn3 and Cyp2b9 were analyzed in female Apoe-deficient mice, no change was observed. Likewise, no significant variation was observed in Apoa1- or in Apoe-deficient mice receiving doses ranging from 0.5 to 5 mg/kg erythrodiol. Our results give evidence that erythrodiol exerts a hepatic transcriptional role, but this is selective in terms of sex and requires a threshold dose. Furthermore, it requires an APOA1-containing HDL.
eIF2α Phosphorylation by GCN2 Is Induced in the Presence of Chitin and Plays an Important Role in Plant Defense against B. cinerea Infection
Authors: Berrocal-Lobo Marta , Toribio René , Castellano M. Mar
Published: October 2020

Translation plays an important role in plant adaptation to different abiotic and biotic stresses; however, the mechanisms involved in translational regulation during each specific response and their effect in translation are poorly understood in plants. In this work, we show that GCN2 promotes eIF2α phosphorylation upon contact with Botrytis cinerea spores, and that this phosphorylation is required for the proper establishment of plant defense against the fungus. In fact, independent gcn2 mutants display an enhanced susceptibility to B. cinerea infection, which is highlighted by an increased cell death and reduced expression of ethylene- and jasmonic-related genes in the gcn2 mutants. eIF2α phosphorylation is not only triggered in the presence of the fungus, but interestingly, is also achieved in the sole presence of the microbe-associated molecular pattern (MAMP) chitin. Moreover, analysis of de novo protein synthesis by 35SMet–35SCys incorporation indicates that chitin treatment promotes a global inhibition of translation. Taken together, these results suggest that eIF2α phosphorylation by GCN2 is promoted in the presence of chitin and plays an important role in plant defense against B. cinerea infection.
Involvement of Mitochondrial Mechanisms in the Cytostatic Effect of Desethylamiodarone in B16F10 Melanoma Cells
Authors: Ramadan Fadi H. J. , Szabo Aliz , Kovacs Dominika , Takatsy Aniko , Bognar Rita , Gallyas Ferenc , Bognar Zita
Published: October 2020

Previously, we showed that desethylamiodarone (DEA), a major metabolite of the widely used antiarrhythmic drug amiodarone, has direct mitochondrial effects. We hypothesized that these effects account for its observed cytotoxic properties and ability to limit in vivo metastasis. Accordingly, we examined DEA’s rapid (3–12 h) cytotoxicity and its early (3–6 h) effects on various mitochondrial processes in B16F10 melanoma cells. DEA did not affect cellular oxygen radical formation, as determined using two fluorescent dyes. However, it did decrease the mitochondrial transmembrane potential, as assessed by JC-1 dye and fluorescence microscopy. It also induced mitochondrial fragmentation, as visualized by confocal fluorescence microscopy. DEA decreased maximal respiration, ATP production, coupling efficiency, glycolysis, and non-mitochondrial oxygen consumption measured by a Seahorse cellular energy metabolism analyzer. In addition, it induced a cyclosporine A–independent mitochondrial permeability transition, as determined by Co2+-mediated calcein fluorescence quenching measured using a high-content imaging system. DEA also caused outer mitochondrial membrane permeabilization, as assessed by the immunoblot analysis of cytochrome C, apoptosis inducing factor, Akt, phospho-Akt, Bad, and phospho-Bad. All of these data supported our initial hypothesis.
Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration
Authors: Mizuno Hiroshi , Fukumoto Masanori , Sato Takaki , Horie Taeko , Kida Teruyo , Oku Hidehiro , Nakamura Kimitoshi , Jin Denan , Takai Shinji , Ikeda Tsunehiko
Published: October 2020

Lattice degeneration involves thinning of the retina that occurs over time. Here we performed an immunohistological study of tissue sections of human peripheral retinal lattice degeneration to investigate if retinal pigment epithelium (RPE) cells are involved in the pathogenesis of this condition. In two cases of retinal detachment with a large tear that underwent vitreous surgery, retinal lattice degeneration tissue specimens were collected during surgery. In the obtained specimens, both whole mounts and horizontal section slices were prepared, and immunostaining was then performed with hematoxylin and antibodies against glial fibrillary acidic protein (GFAP), RPE-specific protein 65 kDa (RPE65), pan-cytokeratin (pan-CK), and CK18. Hematoxylin staining showed no nuclei in the center of the degenerative lesion, thus suggesting the possibility of the occurrence of apoptosis. In the degenerative lesion specimens, GFAP staining was observed in the center, RPE65 staining was observed in the slightly peripheral region, and pan-CK staining was observed in all areas. However, no obvious CK18 staining was observed. In a monkey retina used as the control specimen of a normal healthy retina, no RPE65 or pan-CK staining was observed in the neural retina. Our findings suggest that migration, proliferation, and differentiation of RPE cells might be involved in the repair of retinal lattice degeneration.
The Best Peptidomimetic Strategies to Undercover Antibacterial Peptides
Authors: Lachowicz Joanna Izabela , Szczepski Kacper , Scano Alessandra , Casu Cinzia , Fais Sara , Orrù Germano , Pisano Barbara , Piras Monica , Jaremko Mariusz
Published: October 2020

Health-care systems that develop rapidly and efficiently may increase the lifespan of humans. Nevertheless, the older population is more fragile, and is at an increased risk of disease development. A concurrently growing number of surgeries and transplantations have caused antibiotics to be used much more frequently, and for much longer periods of time, which in turn increases microbial resistance. In 1945, Fleming warned against the abuse of antibiotics in his Nobel lecture: “The time may come when penicillin can be bought by anyone in the shops. Then there is the danger that the ignorant man may easily underdose himself and by exposing his microbes to non-lethal quantities of the drug make them resistant”. After 70 years, we are witnessing the fulfilment of Fleming’s prophecy, as more than 700,000 people die each year due to drug-resistant diseases. Naturally occurring antimicrobial peptides protect all living matter against bacteria, and now different peptidomimetic strategies to engineer innovative antibiotics are being developed to defend humans against bacterial infections.
Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica
Authors: Zheng Peng-Fei , Wang Xun , Yang Yu-Ying , You Chun-Xiang , Zhang Zhen-Lu , Hao Yu-Jin
Published: October 2020

Phytochrome-interacting factors (PIFs), members of the basic helix-loop-helix transcription factor family that have been extensively investigated in Arabidopsis thaliana, play essential roles in plant growth and development. However, PIF members have not been systematically investigated in apples, a worldwide perennial woody crop of economic importance. Here, seven PIF genes were identified from the Malus × domestica reference genome. Chromosomal locations, gene structures, and phylogenetic relationships of these members were analyzed. Analysis of cis-acting elements in promoter regions of MdPIF genes indicated that various elements were related to light, abiotic stress, and plant hormone responsiveness. Subsequently, subcellular localization and transcriptional activity analysis revealed that MdPIFs were typical nuclear transcription factors with transcriptional activation ability. Expression analysis demonstrated that MdPIF genes had different gene expression patterns for various abiotic factors. Moreover, overexpressed MdPIF4 reduced the sensitivity of apple calluses to abscisic acid (ABA). Our work lays foundations for further investigation of PIF functions in plant growth and development in apples.
Microtubule Dysfunction: A Common Feature of Neurodegenerative Diseases
Authors: Sferra Antonella , Nicita Francesco , Bertini Enrico
Published: October 2020

Neurons are particularly susceptible to microtubule (MT) defects and deregulation of the MT cytoskeleton is considered to be a common insult during the pathogenesis of neurodegenerative disorders. Evidence that dysfunctions in the MT system have a direct role in neurodegeneration comes from findings that several forms of neurodegenerative diseases are associated with changes in genes encoding tubulins, the structural units of MTs, MT-associated proteins (MAPs), or additional factors such as MT modifying enzymes which modulating tubulin post-translational modifications (PTMs) regulate MT functions and dynamics. Efforts to use MT-targeting therapeutic agents for the treatment of neurodegenerative diseases are underway. Many of these agents have provided several benefits when tested on both in vitro and in vivo neurodegenerative model systems. Currently, the most frequently addressed therapeutic interventions include drugs that modulate MT stability or that target tubulin PTMs, such as tubulin acetylation. The purpose of this review is to provide an update on the relevance of MT dysfunctions to the process of neurodegeneration and briefly discuss advances in the use of MT-targeting drugs for the treatment of neurodegenerative disorders.
Transcriptome Analysis of Psacothea hilaris: De Novo Assembly and Antimicrobial Peptide Prediction
Authors: Lee Joon Ha , Chung Hoyong , Shin Yong Pyo , Kim In-Woo , Natarajan Sathishkumar , Veerappan Karpagam , Seo Minchul , Park Junhyung , Hwang Jae Sam
Published: October 2020

Antimicrobial peptides (AMPs) are the frontline innate defense system evolutionarily preserved in insects to combat invading pathogens. These AMPs could serve as an alternative to classical antibiotics to overcome the burden of treating multidrug resistant bacteria. Psacotheasin, a knottin type AMP was isolated from Psacothea hilaris and shown to exhibit antimicrobial activity, especially against fungi through apoptosis mediated cell death. In this study, we aimed to identify novel probable AMPs from Psacothea hilaris, the yellow spotted longicorn beetle. The beetle was immunized with the two bacterial strains (E. coli and S. aureus), and the yeast strain C. albicans. After immunization, total RNA was isolated and sequenced in Illumina platform. Then, beetle transcriptome was de novo assembled and searched for putative AMPs with the known physiochemical features of the AMPs. A selection of AMP candidates were synthesized and tested for antimicrobial activity. Four peptides showed stronger activity against E. coli than the control AMP, melittin while one peptide showed similar activity against S. aureus. Moreover, four peptides and two peptides showed antifungal activity stronger than and similar to melittin, respectively. Collectively one peptide showed both antibacterial and antifungal activity superior to melittin; thus, it provides a potent antimicrobial peptide. All the peptides showed no hemolysis in all the tested concentrations. These results suggest that in silico mining of insects’ transcriptome could be a promising tool to obtain and optimize novel AMPs for human needs.
Systemic Urate Deposition: An Unrecognized Complication of Gout?
Authors: Khanna Puja , Johnson Richard J. , Marder Bradley , LaMoreaux Brian , Kumar Ada
Published: October 2020

Gout, an inflammatory arthritis, affects over nine million people in the US with increasing prevalence. Some medical societies do not recommend treating gout unless it is recurrent. While soft tissue urate deposits (tophi), resultant bone erosions, and joint inflammation are frequently recognized in gout, urate crystal deposits in other sites have been thought to be rare. Recent diagnostic testing, such as dual energy computed tomography (DECT), has led to the recognition that urate deposits are not uncommon in other tissues including the vasculature. To understand the potential risks for untreated gout, we reviewed the literature on extra-articular urate deposition documented by autopsy, histopathology, surgery, and radiology, including the heart, blood vessels, kidney, spine, eye, skin, and gastrointestinal system. These studies extend the significance of gout beyond the rheumatologist and emphasize the need for physicians to follow the American College of Rheumatology guidelines to treat subjects with gout to a goal of achieving serum urate <6 mg/dl. Given the growing body of literature on extraarticular urate deposition, further studies and clinical trials are needed to determine the clinical consequences of systemic urate deposition, including if reducing cardiac and vascular urate deposits may provide a survival benefit for this at-risk population.
Polyphenol Bioavailability and Plasma Antiradical Capacity in Healthy Subjects after Acute Intake of Pigmented Rice: A Crossover Randomized Controlled Clinical Trial
Authors: Vitalini Sara , Sardella Andrea , Fracassetti Daniela , Secli Rita , Tirelli Antonio , Lodi Giovanni , Carrassi Antonio , Varoni Elena Maria , Iriti Marcello
Published: October 2020

Health-promoting effects of plant foods have been emphasized in the last few decades and ascribed to the bioactive phytochemicals present therein—in particular, phenylpropanoids. The latter have been investigated for a number of preclinical biological activities, including their antioxidant power. Due to the paucity of human studies, in this randomized intervention trial, we investigated whether the acute intake of pigmented rice could increase the plasma bioactive levels and antiradical power in twenty healthy subjects. A crossover randomized controlled clinical trial was conducted on 19 volunteers. Artemide and Venere black rice cultivars were tested, while brown rice Carnaroli was used as a control. Each patient received randomly one serving (100 g) of rice on three different experimental days, separated by a 7-day washout period. After baseline blood withdrawal, time-course changes of plasma polyphenols, flavonoids and radical-scavenging capacity were determined at 30, 60, 120 and 180 min post rice intake. Compared to Carnaroli rice, the two black rice cultivars significantly increased the plasma levels of polyphenols and flavonoids at 60 and 120 min and, correspondingly, the plasma antiradical power at 60 min after consumption. Pigmented rice consumption can contribute to diet-related health benefits in humans.
Validity of Virtual Reality Body Exposure to Elicit Fear of Gaining Weight, Body Anxiety and Body-Related Attentional Bias in Patients with Anorexia Nervosa
Authors: Porras-Garcia Bruno , Ferrer-Garcia Marta , Serrano-Troncoso Eduardo , Carulla-Roig Marta , Soto-Usera Pau , Miquel-Nabau Helena , Shojaeian Nazilla , de la Montaña Santos-Carrasco Isabel , Borszewski Bianca , Díaz-Marsá Marina , Sánchez-Díaz Isabel , Fernández-Aranda Fernando , Gutiérrez-Maldonado José
Published: October 2020

Fear of gaining weight (FGW), body image disturbances, associated anxiety and body-related attentional bias are the core symptoms of anorexia nervosa (AN) and play critical roles in its development and maintenance. The aim of the current study is to evaluate the usefulness of virtual reality-based body exposure software for the assessment of important body-related cognitive and emotional responses in AN. Thirty female patients with AN, one of them subclinical, and 43 healthy college women, 25 with low body dissatisfaction (BD) and 18 with high BD, owned a virtual body that had their silhouette and body mass index. Full-body illusion (FBI) over the virtual body was induced using both visuo-motor and visuo-tactile stimulation. Once the FBI was induced, the FBI itself, FGW, body anxiety and body-related attentional bias toward weight-related and non-weight-related body areas were assessed. One-way analyses of covariance (ANCOVA), controlling for age, showed that AN patients reported higher FGW, body anxiety and body-related attentional bias than healthy controls. Unexpectedly, patients with AN reported significantly lower FBI levels than healthy participants. Finally, Pearson correlations showed significant relationships between visual analog scales and body-related attentional bias measures, compared to other eating disorder measures. These results provide evidence about the usefulness of virtual reality-based body exposure to elicit FGW and other body-related disturbances in AN patients. Thus, it may be a suitable intervention for reducing these emotional responses and for easing weight recovery.
Assessing Cyber Challenges of Maritime Navigation
Authors: Androjna Andrej , Brcko Tanja , Pavic Ivica , Greidanus Harm
Published: October 2020

This paper provides a close investigation into the landscape of both cyber threats and actual incidents in the maritime sector, identifying the cyber trends and challenges as they relate to safe navigation and marine shipping. As an important subset of cyber threats that impact many maritime systems, the vulnerabilities of satellite navigation systems, in particular the Global Positioning System (GPS), receive special attention. For this article, a systematic literature review was conducted, complemented by the research and analysis of a specific spoofing event. Analyzing available resources, we might summarize that a shift in mind-set is essential to direct more attention and resources toward cybersecurity as well as the necessity for manufacturers to improve the cybersecurity of their products, as shipping systems currently remain vulnerable to cybercriminals. There is a need for multiple positioning, navigation, and timing (PNT) systems onboard maritime vessels to complement GPS-only navigation. The use of multiple satellite navigation constellations, public as well as private, in combination with the terrestrial components of an enhanced LOng-RAnge Navigation (eLoran) system and ports’ laser-based aid system for berthing and docking should provide the shipping industry with the direly needed increased protection from cyber-attackers for the foreseeable future.
Selected Papers from the Second International Conference on Modelling and Optimisation of Ship Energy Systems (MOSES2019)
Authors: Theotokatos Gerasimos , Coraddu Andrea
Published: October 2020

This Special Issue presents a collection of articles addressing the contemporary challenges in the areas of the ship energy systems modelling and optimisation [...]
The Interaction of Functional Predictors and the Mechanical Predictor Perseveration in a Variationist Analysis of Caribbean Spanish Heritage Speaker Subject Pronoun Expression
Authors: de Prada Pérez Ana
Published: October 2020

Subject pronoun expression (SPE) in Spanish has been widely studied across monolingual and bilingual varieties, showing a consistent effect of functional predictors. In recent papers, the role of the mechanical predictor priming, or perseveration, has been the source of debate. Additionally, little is known about the interaction of perseveration and significant functional predictors (e.g., grammatical person). In this paper, we expand on previous research by examining first-person singular (1sg) and third-person singular (3sg) data from sociolinguistic interviews with Spanish–English bilinguals from Florida to explore the possible difference in priming in deictic vs. referential subjects. The results from a mixed-effects variable rule analysis only offered clear evidence of priming in 1sg. We hypothesize that this result could be due to either surprisal (1sg overt pronominal subjects are rarer in the corpus that 3sg overt pronominal subjects) or to 3sg involving reference-tracking and perseveration only being evident in contexts where the subject form does not signal for pragmatic content.
Temperature Field in the Heat Transfer Process of PEEK Thermoplastic Composite Fiber Placement
Authors: Cao Zhongliang , Dong Mingjun , Liu Kailei , Fu Hongya
Published: October 2020

Under the effect of different process parameters, the temperature field inside the thermoplastic fiber is very complex and directly affects the fusion quality between the resins. Considering the heat transfer behavior of thermoplastic fiber polyether ether ketone (PEEK) as the research object, a mathematical model of heat transfer in the thermoplastic composite fiber placement with the relevant boundary conditions was established. Ansys Parametric Design Language (APDL) was used to generate the finite element model and simulate the transient process, not only to explore the influence of various process parameters on the temperature field, but also to build an online temperature field measurement system. The influence rules of placement process parameters and mold initial temperature with respect to the temperature field in the first layer were obtained. Combining the relationship between heating temperature and placement speed, when the first layer was laid, the placement process temperature could be quickly reached by low speed and high temperature. The temperature data were collected by the online detection system. Compared with the temperature data from the simulation, the error was below 8%, which verified the correctness of the heat transfer model. The academic research results will lay a theoretical foundation for the thermoplastic fiber placement.
The Galvanic Effect of Titanium and Amalgam in the Oral Environment
Authors: Carey IV Patrick H. , Hsu Shu-Min , Fares Chaker , Kamenov George , Ren Fan , Esquivel-Upshaw Josephine
Published: October 2020

The effects of the presence of amalgam on titanium (Ti) dissolution in the oral environment under acidic, neutral, and basic conditions was studied. The presence of amalgam was found to suppress Ti release under acidic conditions due to the redeposition of TiOx/SnOx on the surface of the Ti. The redeposition of SnOx was due to the amalgam releasing its components (Hg, Cu, Sn, Ag) and the thermodynamic preference of Sn to oxidize, which was confirmed using mass measurements, ICP-MS analyses, and X-ray Photoelectron Spectroscopy (XPS). XPS depth profiling was performed to characterize the composition and oxidation states of the redeposited SnOx/TiOx film. Under basic conditions, the amalgam hindered Ti dissolution, but no redeposition of amalgam components was observed for the Ti.
Effects of Temperature on the Morphology and Optical Properties of Spark Discharge Germanium Nanoparticles
Authors: Lizunova Anna , Mazharenko Anastasia , Masnaviev Bulat , Khramov Egor , Efimov Alexey , Ramanenka Andrei , Shuklov Ivan , Ivanov Viktor
Published: October 2020

We report the spark discharge synthesis of aerosol germanium nanoparticles followed by sintering in a tube furnace at different temperatures varying from 25 to 800 °C. The size, structure, chemical composition and optical properties were studied. We have demonstrated a melting mechanism of nanoparticles agglomerates, the growth of the mean primary particle size from 7 to 51 nm and the reduction of the size of agglomerates with a temperature increase. According to transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) data, primary nanoparticles sintered at temperatures from 25 to 475 °C basically have a structure of Ge crystals embedded in a GeOx amorphous matrix, as well as visible photoluminescence (PL) with the maximum at 550 nm. Pure germanium nanoparticles are prepared at temperatures above 625 °C and distinguished by their absence of visible PL. The shape of the experimental UV-vis-NIR extinction spectra significantly depends on the size distribution of the germanium crystals. This fact was confirmed by simulations according to Mie theory for obtained ensembles of germanium nanoparticles.
A Two-Phase Model for Personnel Selection Based on Multi-Type Fuzzy Information
Authors: Chen Chen-Tung , Hung Wei-Zhan
Published: October 2020

From the viewpoint of human resource management, personnel selection is one of the more important issues for enterprises in a high-level competitive environment. In general, many influence factors, quantitative and qualitative, affect the decision-making process of personnel selection. For considering qualitative factors, decision-makers cannot always easily judge the suitable degree of each applicant. Under this situation, this research proposes a systematic decision-making method based on computing with linguistic variables. First, unsuitable applicants are filtered by considering the quantitative information of each applicant. At this stage, technique for order of preference by similarity to ideal solution (TOPSIS) and entropy methods are aggregated to eliminate unsuitable applicants in accordance with their closeness coefficient values. Second, experts (or decision-makers) use different types of 2-tuple linguistic variables to express their opinions of suitable candidates with respect to qualitative criteria. At this stage, we consider different preference functions in the preference ranking organization method for enrichment evaluation (PROMETHEE) method to calculate the outranking index of each suitable candidate. Next, we aggregate the closeness coefficient and outranking index of each suitable applicant to determine the ranking order. In order to illustrate the computational processes, an example demonstrates the practicability of the two-phase personnel selection method. The benefit of the proposed method is as follows. (1) It reduces the time for reviewing and evaluating the huge numbers of applicants. (2) It avoids subjective judgment by experts to determine the weights of all criteria. Finally, conclusions and contributions are discussed at the end of this paper.
Finite-Time H∞ Static Output Feedback Control for Itô Stochastic Markovian Jump Systems
Authors: Xikui Liu , Yapeng Teng , Yan Li
Published: October 2020

This paper focuses on the problem of finite-time H∞ static output feedback control for Ito^ stochastic systems with Markovian jumps (MJs). First of all, by introducing a new state vector and a novel signal, several sufficient conditions for the existence of static output feedback controllers are established for the considered systems with completely known transition rates (CKTRs) and partially known transition rates (PKTRs), respectively. Then the static output feedback controllers are designed via solving linear matrix inequalities (LMIs), which ensure the closed-loop systems are stochastic H∞ finite-time boundedness. The validity of the developed method was demonstrated through two examples.
Engineering Applications of Peristaltic Fluid Flow with Hall Current, Thermal Deposition and Convective Conditions
Authors: Yasmin Humaira , Iqbal Naveed , Tanveer Anum
Published: October 2020

This article addresses the peristaltic flow in a compliant wall channel. Analysis has been carried out in the presence of a Hall current and chemical reaction. Convective conditions in terms of both heat and mass transfer are employed. Mathematical modeling is developed for an incompressible Carreau fluid. Thermal deposition effect and convection at the channel walls are considered. Series solutions are obtained for small Weissenberg number We. Solution expressions of velocity, temperature, concentration and stream function are obtained. These physical quantities are displayed and analyzed. Heat transfer coefficient and trapping are explored in detail.
Applying Membrane Mode Enhanced Cohesive Zone Elements on Tailored Forming Components
Authors: Töller Felix , Löhnert Stefan , Wriggers Peter
Published: October 2020

Forming of hybrid bulk metal components might include severe membrane mode deformation of the joining zone. This effect is not reflected by common Traction Separation Laws used within Cohesive Zone Elements that are usually applied for the simulation of joining zones. Thus, they cannot capture possible damage of the joining zone under these conditions. Membrane Mode Enhanced Cohesive Zone Elements fix this deficiency. This novel approach can be implemented in finite elements. It can be used within commercial codes where an implementation as a material model is beneficial as this simplifies model preparation with the existing GUIs. In this contribution, the implementation of Membrane Mode Enhanced Cohesive Zone Elements as a material model is presented within MSC Marc along with simulations showing the capabilities of this approach.
A Scalable and Low Stress Post-CMOS Processing Technique for Implantable Microsensors
Authors: Lee Ah-Hyoung , Lee Jihun , Laiwalla Farah , Leung Vincent , Huang Jiannan , Nurmikko Arto , Song Yoon-Kyu
Published: October 2020

Implantable active electronic microchips are being developed as multinode in-body sensors and actuators. There is a need to develop high throughput microfabrication techniques applicable to complementary metal–oxide–semiconductor (CMOS)-based silicon electronics in order to process bare dies from a foundry to physiologically compatible implant ensembles. Post-processing of a miniature CMOS chip by usual methods is challenging as the typically sub-mm size small dies are hard to handle and not readily compatible with the standard microfabrication, e.g., photolithography. Here, we present a soft material-based, low chemical and mechanical stress, scalable microchip post-CMOS processing method that enables photolithography and electron-beam deposition on hundreds of micrometers scale dies. The technique builds on the use of a polydimethylsiloxane (PDMS) carrier substrate, in which the CMOS chips were embedded and precisely aligned, thereby enabling batch post-processing without complication from additional micromachining or chip treatments. We have demonstrated our technique with 650 μm × 650 μm and 280 μm × 280 μm chips, designed for electrophysiological neural recording and microstimulation implants by monolithic integration of patterned gold and PEDOT:PSS electrodes on the chips and assessed their electrical properties. The functionality of the post-processed chips was verified in saline, and ex vivo experiments using wireless power and data link, to demonstrate the recording and stimulation performance of the microscale electrode interfaces.
Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus
Authors: Takadate Yoshihiro , Manzoor Rashid , Saito Takeshi , Kida Yurie , Maruyama Junki , Kondoh Tatsunari , Miyamoto Hiroko , Ogawa Hirohito , Kajihara Masahiro , Igarashi Manabu , Takada Ayato
Published: October 2020

Lloviu virus (LLOV), a bat-derived filovirus that is phylogenetically distinct from human pathogenic filoviruses such as Ebola virus (EBOV) and Marburg virus (MARV), was discovered in Europe. However, since infectious LLOV has never been isolated, the biological properties of this virus remain poorly understood. We found that vesicular stomatitis virus (VSV) pseudotyped with the glycoprotein (GP) of LLOV (VSV–LLOV) showed higher infectivity in one bat (Miniopterus sp.)-derived cell line than in the other bat-derived cell lines tested, which was distinct from the tropism of VSV pseudotyped with EBOV (VSV–EBOV) and MARV GPs. We then focused on the interaction between GP and Niemann–Pick C1 (NPC1) protein, one of the cellular receptors of filoviruses. We introduced the Miniopterus bat and human NPC1 genes into NPC1-knockout Vero E6 cells and their susceptibilities to the viruses were compared. The cell line expressing the bat NPC1 showed higher susceptibility to VSV–LLOV than that expressing human NPC1, whereas the opposite preference was seen for VSV–EBOV. Using a site-directed mutagenesis approach, amino acid residues involved in the differential tropism were identified in the NPC1 and GP molecules. Our results suggest that the interaction between GP and NPC1 is an important factor in the tropism of LLOV to a particular bat species.