bluuebunny/crossref_metadata_2025 huggingface dataset:
An Ownership Verification Mechanism Against Encrypted Forwarding Attacks in Data-Driven Social Computing
Authors: Sun Zhe , Wan Junping , Wang Bin , Cao Zhiqiang , Li Ran , He Yuanyuan
Published: September 2021

Data-driven deep learning has accelerated the spread of social computing applications. To develop a reliable social application, service providers need massive data on human behavior and interactions. As the data is highly relevant to users’ privacy, researchers have conducted extensive research on how to securely build a collaborative training model. Cryptography methods are an essential component of collaborative training which is used to protect privacy information in gradients. However, the encrypted gradient is semantically invisible, so it is difficult to detect malicious participants forwarding other’s gradient to profit unfairly. In this paper, we propose a data ownership verification mechanism based on Σ-protocol and Pedersen commitment, which can help prevent gradient stealing behavior. We deploy the Paillier algorithm on the encoded gradient to protect privacy information in collaborative training. In addition, we design a united commitment scheme to complete the verification process of commitments in batches, and reduce verification consumption for aggregators in large-scale social computing. The evaluation of the experiments demonstrates the effectiveness and efficiency of our proposed mechanism.
Hybrid MDM-PDM Based Ro-FSO System for Broadband Services by Incorporating Donut Modes Under Diverse Weather Conditions
Authors: Chaudhary Sushank , Wuttisittikulkij Lunchakorn , Nebhen Jamel , Tang Xuan , Saadi Muhammad , Al Otaibi Sattam , Althobaiti Ahmed , Sharma Abhishek , Choudhary Sunita
Published: September 2021

High-speed data demand in sensitive locations has prompted new wireless technologies to grow in areas like hospitals for bio-sensor data transmission between doctors and patients. However, interference of electromagnetic spectrum or highly sensitive medical equipment in such locations can prevent radio waves which can further compromise the health of patients. Radio over Free Space Optics (Ro-FSO) can fulfil high-speed data demand in such locations without any such interference. However, the Ro-FSO performance is highly influenced by different adverse weather conditions, particularly haze and rainfall, which further cause attenuation in the transmission path of Ro-FSO systems. These atmospheric turbulences mainly affect the transmission link range of Ro-FSO systems. In this work, Ro-FSO system is designed by incorporating hybrid mode division multiplexing (MDM) and polarization division multiplexing (PDM) schemes to deliver four independent channels, each carrying 10 Gbps data upconverted to 40 GHz radio signal, over 3.4 km free space optical link operating under clear weather conditions. In addition to this, the proposed Ro-FSO link is subjected to different weather conditions, particularly partially hazy/rainy and dense fog/very rainy. The reported results indicate the achievement of acceptable bit error rate (BER≈10–3) for all channels up to 3400m FSO link under clear weather conditions, 1000m under partially haze/rain and 620 m under dense fog/heavy rain.
Saccharification Potential of Transgenic Greenhouse- and Field-Grown Aspen Engineered for Reduced Xylan Acetylation
Authors: Pramod Sivan , Gandla Madhavi Latha , Derba-Maceluch Marta , Jönsson Leif J. , Mellerowicz Ewa J. , Winestrand Sandra
Published: September 2021

High acetylation of xylan in hardwoods decreases their value as biorefinery feedstocks. To counter this problem, we have constitutively suppressed RWA genes encoding acetyl-CoA transporters using the 35S promoter, or constitutively and wood-specifically (using the WP promoter) expressed fungal acetyl xylan esterases of families CE1 (AnAXE1) and CE5 (HjAXE), to reduce acetylation in hybrid aspen. All these transformations improved the saccharification of wood from greenhouse-grown trees. Here, we describe the chemical properties and saccharification potential of the resulting lines grown in a five-year field trial, and one type of them (WP:AnAXE1) in greenhouse conditions. Chemically, the lignocellulose of the field- and greenhouse-field-grown plants slightly differed, but the reductions in acetylation and saccharification improvement of engineered trees were largely maintained in the field. The main novel phenotypic observation in the field was higher lignification in lines with the WP promoter than those with the 35S promoter. Following growth in the field, saccharification glucose yields were higher from most transformed lines than from wild-type (WT) plants with no pretreatment, but there was no improvement in saccharification with acid pretreatment. Thus, acid pretreatment removes most recalcitrance caused by acetylation. We found a complex relationship between acetylation and glucose yields in saccharification without pretreatment, suggesting that other variables, for example, the acetylation pattern, affect recalcitrance. Bigger gains in glucose yields were observed in lines with the 35S promoter than in those with the WP promoter, possibly due to their lower lignin content. However, better lignocellulose saccharification of these lines was offset by a growth penalty and their glucose yield per tree was lower. In a comparison of the best lines with each construct, WP:AnAXE1 provided the highest glucose yield per tree from saccharification, with and without pretreatment, WP:HjAXE yields were similar to those of WT plants, and yields of lines with other constructs were lower. These results show that lignocellulose properties of field-grown trees can be improved by reducing cell wall acetylation using various approaches, but some affect productivity in the field. Thus, better understanding of molecular and physiological consequences of deacetylation is needed to obtain quantitatively better results.
A Method of Green Citrus Detection in Natural Environments Using a Deep Convolutional Neural Network
Authors: Zheng Zhenhui , Xiong Juntao , Lin Huan , Han Yonglin , Sun Baoxia , Xie Zhiming , Yang Zhengang , Wang Chenglin
Published: September 2021

The accurate detection of green citrus in natural environments is a key step in realizing the intelligent harvesting of citrus through robotics. At present, the visual detection algorithms for green citrus in natural environments still have poor accuracy and robustness due to the color similarity between fruits and backgrounds. This study proposed a multi-scale convolutional neural network (CNN) named YOLO BP to detect green citrus in natural environments. Firstly, the backbone network, CSPDarknet53, was trimmed to extract high-quality features and improve the real-time performance of the network. Then, by removing the redundant nodes of the Path Aggregation Network (PANet) and adding additional connections, a bi-directional feature pyramid network (Bi-PANet) was proposed to efficiently fuse the multilayer features. Finally, three groups of green citrus detection experiments were designed to evaluate the network performance. The results showed that the accuracy, recall, mean average precision (mAP), and detection speed of YOLO BP were 86, 91, and 91.55% and 18 frames per second (FPS), respectively, which were 2, 7, and 4.3% and 1 FPS higher than those of YOLO v4. The proposed detection algorithm had strong robustness and high accuracy in the complex orchard environment, which provides technical support for green fruit detection in natural environments.
Translation, Adaption, and Psychometric Testing of the Myanmar Version of the Medical Outcomes Study Social Support Survey for People Living With HIV/AIDS
Authors: Huang Feifei , Chen Wei-Ti , Lin Sai Htun , Tun Min San , Nwe Thet Wai , Oo Yin Thet Nu , Oo Htun Nyunt
Published: September 2021

Introduction: Valid and reliable instruments are crucial for measuring perceived social support among people living with HIV (PLHIV). We aimed to investigate the psychometric properties of the English version of the 19-item Medical Outcomes Study Social Support Survey (MOS-SSS) adapted for PLHIV in Myanmar.

Methods: Based on a standard cross-cultural procedure, we adapted the MOS-SSS and formed a Myanmar version of the scale (MOS-SSS-M), and then tested its validity and reliability. A sample of 250 eligible PLHIV was collected from a closed Facebook group that included more than 10,000 Myanmars, most of whom were PLHIV.

Results: The MOS-SSS-M achieved a Cronbach’s α of 0.82–0.95. Confirmatory factor analysis revealed an acceptable fit index for the four-factor structure. Construct validity was demonstrated by significant association with self-reported HIV stigma and stress levels, and further confirmed by the findings of Rasch analysis.

Conclusion: The MOS-SSS-M with a four-factor structure can be used to measure the level and categories of perceived social support among PLHIV in Myanmar.
Individual Differences in Different Measures of Opioid Self-Administration in Rats Are Accounted for by a Single Latent Variable
Authors: Swain Yayi , Waller Niels G. , Gewirtz Jonathan C. , Harris Andrew C.
Published: September 2021

Individual differences in vulnerability to addiction have been widely studied through factor analysis (FA) in humans, a statistical method that identifies “latent” variables (variables that are not measured directly) that reflect the common variance among a larger number of observed measures. Despite its widespread application in behavioral genetics, FA has not been used in preclinical opioid addiction research. The current study used FA to examine the latent factor structure of four measures of i.v. morphine self-administration (MSA) in rats (i.e., acquisition, demand elasticity, morphine/cue- and stress/cue-induced reinstatement). All four MSA measures are generally assumed in the preclinical literature to reflect “addiction vulnerability,” and individual differences in multiple measures of abuse liability are best accounted for by a single latent factor in some human studies. A one-factor model was therefore fitted to the data. Two different regularized FAs indicated that a one-factor model fit our data well. Acquisition, elasticity of demand and morphine/cue-induced reinstatement loaded significantly onto a single latent factor while stress/cue-induced reinstatement did not. Consistent with findings from some human studies, our results indicated a common drug “addiction” factor underlying several measures of opioid SA. However, stress/cue-induced reinstatement loaded poorly onto this factor, suggesting that unique mechanisms mediate individual differences in this vs. other MSA measures. Further establishing FA approaches in drug SA and in preclinical neuropsychopathology more broadly will provide more reliable, clinically relevant core factors underlying disease vulnerability in animal models for further genetic analyses.
Association of Circulating ANGPTL8 Levels With Renal Dysfunction: A Case-Control Study
Authors: Meng Xiaoyu , Zou Huajie , Li Danpei , Yu Peng , Huang Li , Zhang Jianhua , Li Wenjun , Yu Xuefeng
Published: September 2021

Background: Chronic kidney disease (CKD) is recognized as a major public health problem with high morbidity and mortality worldwide. Recently, angiopoietin-like protein 8 (ANGPTL8) was found to regulate lipid metabolism. Previous studies suggested that serum ANGPTL8 levels increased in patients with diabetes, especially in diabetic patients with albuminuria. This study aimed to investigate the association between circulating levels of ANGPTL8 and kidney function in the general population.

Methods: The subjects were patients with renal dysfunction [estimated glomerular filtration rate (eGFR) <60/min/1.73 m2] from Risk Evaluation of cAncers in Chinese diabeTic Individuals: a lONgitudinal study (the REACTION study). Each case was matched by age, sex, and body mass index (BMI) with one control whose eGFR was ≥ 90 ml/min/1.73 m2. The case and control groups were compared using a paired t-test. Binary logistic regression analysis was used to calculate the odds ratio (OR) of renal dysfunction (RD).

Results: Among 135 case-control pairs, circulating ANGPTL8 levels were elevated in patients with RD compared to control subjects [799.96 (410.12-1086.44) vs. 609.58 (365.13-740.06) pg/ml, p < 0.05]. Partial correlations showed that ANGPTL8 levels were negatively correlated with eGFR (r = −0.26, p < 0.05). Multivariable-adjusted binary logistic regression analysis showed that elevated ANGPTL8 levels were associated with an increased risk of RD (OR in quartile 4 vs. 1, 3.80; 95% CI, 1.71-8.41). Interestingly, the association between ANGPTL8 levels and RD was consistent with the overall findings in both nondiabetic individuals (OR, 1.44; 95% CI, 1.09 to 1.91) and diabetic patients (OR, 2.71; 95% CI, 1.13-6.49) in the subgroup analyses. Furthermore, the estimates for this association were also significant in females (OR, 2.12; 95% CI, 1.33-3.37), individuals aged > 60 years (OR, 1.55; 95% CI, 1.16-2.07), individuals with a BMI <24 (OR, 1.66; 95% CI, 1.16-2.39), and individuals without hyperlipidaemia (OR, 1.61; 95% CI, 1.16-2.23) (all p-values <0.05).

Conclusion: Elevated circulating ANGPTL8 levels were associated with increased risk of RD in the general population, especially among females, individuals aged > 60 years, individuals with a BMI < 24, individuals without diabetes mellitus, individuals with diabetes mellitus (DM), and individuals without hyperlipidaemia. This finding implies that ANGPTL8 may play a role in the pathological process of RD.
Current Perspectives of Artificial Intelligence in Pediatric Neuroradiology: An Overview
Authors: Martin Dann , Tong Elizabeth , Kelly Brendan , Yeom Kristen , Yedavalli Vivek
Published: September 2021

Artificial Intelligence, Machine Learning, and myriad related techniques are becoming ever more commonplace throughout industry and society, and radiology is by no means an exception. It is essential for every radiologists of every subspecialty to gain familiarity and confidence with these techniques as they become increasingly incorporated into the routine practice in both academic and private practice settings. In this article, we provide a brief review of several definitions and techniques that are commonly used in AI, and in particular machine vision, and examples of how they are currently being applied to the setting of clinical neuroradiology. We then review the unique challenges that the adoption and application of faces within the subspecialty of pediatric neuroradiology, and how these obstacles may be overcome. We conclude by presenting specific examples of how AI is currently being applied within the field of pediatric neuroradiology and the potential opportunities that are available for future applications.
The Effect of Laparoscopic Radical Hysterectomy Surgical Volume on Oncology Outcomes in Early-Stage Cervical Cancer
Authors: Li Pengfei , Liu Jiaqi , Wang Li , Kang Shang , Yang Ying , Guo Jianxin , Yao Jilong , Lu Anwei , Wang Zhonghai , Lin Bin , Li Zhiqiang , Bin Xiaonong , Lang Jinghe , Liu Ping , Chen Chunlin
Published: September 2021

Purpose: To examine the association between surgical volume and surgical and oncological outcomes of women with stage IB1 cervical cancer who underwent laparoscopic radical hysterectomy (LRH).

Methods: We retrospectively analyzed the oncological outcomes of 1,137 patients with stage IB1 cervical cancer receiving LRH from 2004 to 2016. The surgical volume for each surgeon was defined as low [fewer than 50 surgeries, n = 392(34.5%)], mid [51-100 surgeries, n = 315(27.7%)], and high [100 surgeries or more, n = 430(37.8%)]. Surgical volume-specific survival was examined with Kaplan–Meier analysis, multivariable analysis, and propensity score matching.

Results: The operative times of the high-volume group (227.35 ± 7.796 min) were significantly shorter than that of the low- (272.77 ± 4.887 min, p < 0.001) and mid-volume (255.86 ± 4.981 min, p < 0.001) groups. Blood loss in the high-volume group (169.42 ± 8.714 ml) was significantly less than that in the low-volume group (219.24 ± 11.299 ml, p = 0.003). The 5-year disease-free survival (DFS) and overall survival (OS) in the low-volume, mid-volume, and high-volume groups were similar (DFS: 91.9, 86.7, and 89.2%, p = 0.102; OS: 96.4, 93.5, and 94.2%, p = 0.192). Multivariable analysis revealed surgical volume was not an independent risk factor for OS or DFS. The rate of intraoperative and postoperative complications was similar among the three groups (p = 0.210).

Conclusions: Surgical volume of LRH may not be a prognostic factor for patients with stage IB1 cervical cancer. Surgery at high-volume surgeon is associated with decreased operative time and blood loss.
Presence of Adult Companion Goats Favors the Rumen Microbial and Functional Development in Artificially Reared Kids
Authors: Palma-Hidalgo Juan Manuel , Yáñez-Ruiz David R. , Jiménez Elisabeth , Martín-García A. Ignacio , Belanche Alejandro
Published: September 2021

Newborn dairy ruminants are usually separated from their dams after birth and fed on milk replacer. This lack of contact with adult animals may hinder the rumen microbiological and physiological development. This study evaluates the effects of rearing newborn goat kids in contact with adult companions on the rumen development. Thirty-two newborn goat kids were randomly allocated to two experimental groups which were reared either in the absence (CTL) or in the presence of non-lactating adult goats (CMP) and weaned at 7 weeks of age. Blood and rumen samples were taken at 5, 7, and 9 weeks of age to evaluate blood metabolites and rumen microbial fermentation. Next-generation sequencing was carried out on rumen samples collected at 7 weeks of age. Results showed that CTL kids lacked rumen protozoa, whereas CMP kids had an abundant and complex protozoal community as well as higher methanogen abundance which positively correlated with the body weight and blood β-hydroxybutyrate as indicators of the physiological development. CMP kids also had a more diverse bacterial community (+132 ASVs) and a different structure of the bacterial and methanogen communities than CTL kids. The core rumen bacterial community in CMP animals had 53 more ASVs than that of CTL animals. Furthermore, the number of ASVs shared with the adult companions was over 4-fold higher in CMP kids than in CTL kids. Greater levels of early rumen colonizers Proteobacteria and Spirochaetes were found in CTL kids, while CMP kids had higher levels of Bacteroidetes and other less abundant taxa (Veillonellaceae, Cyanobacteria, and Selenomonas). These findings suggest that the presence of adult companions facilitated the rumen microbial development prior to weaning. This accelerated microbial development had no effect on the animal growth, but CMP animals presented higher rumen pH and butyrate (+45%) and ammonia concentrations than CTL kids, suggesting higher fibrolytic and proteolytic activities. CMP kids also had higher blood β-hydroxybutyrate (+79%) and lower blood glucose concentrations (-23%) at weaning, indicating an earlier metabolic development which could favor the transition from pre-ruminant to ruminant after the weaning process. Further research is needed to determine the effects of this intervention in more challenging farm conditions.
Hybrid Rockets as Post-Boost Stages and Kick Motors
Authors: Kamps Landon , Hirai Shota , Nagata Harunori
Published: September 2021

Hybrid rockets are attractive as post-boost stages and kick motors due to their inherent safety and low cost, but it is not clear from previous research which oxidizer is most suitable for maximizing ΔV within a fixed envelope size, or what impact O/F shift and nozzle erosion will have on ΔV. A standard hybrid rocket design is proposed and used to clarify the impact of component masses on ΔV within three 1 m3 envelopes of varying height-to-base ratios. Theoretical maximum ΔV are evaluated first, assuming constant O/F and no nozzle erosion. Of the four common liquid oxidizers: H2O2 85 wt%, N2O, N2O4, and LOX, H2O2 85 wt% is shown to result in the highest ΔV, and N2O is shown to result in the highest density ΔV, which is the ΔV normalized for motor density. When O/F shift is considered, the ΔV decreases by 9% for the N2O motor and 12% for the H2O2 85 wt% motor. When nozzle erosion is also considered, the ΔV decreases by another 7% for the H2O2 85 wt% motor and 4% for the N2O motor. Even with O/F shift and nozzle erosion, the H2O2 85 wt% motor can accelerate itself (916 kg) upwards of 4000 m/s, and the N2O motor (456 kg) 3550 m/s.
Genotype, Environment, Year, and Harvest Effects on Fruit Quality Traits of Five Blueberry (Vaccinium corymbosum L.) Cultivars
Authors: Redpath Lauren E. , Gumpertz Marcia , Ballington James R. , Bassil Nahla , Ashrafi Hamid
Published: September 2021

Blueberries (Vaccinium spp.) comprise a broad range of perennial woody species. Introgression of native species into cultivated germplasm has adapted Vaccinium germplasm to a range of climates and growing conditions for cultivated blueberry. Genetic differences signify phenotypic variance that is observed among blueberry accessions. In addition, variability in geographic and climatic growing conditions between environments or within the same environment across different years may further affect fruit and plant phenotypic expression. As a result, a phenotype is a function of genetic background (G), environment (E), and their interaction (G × E). In addition, other temporally regulated factors such as year (Y) and harvest time (H) impact plant and fruit quality phenotypic variation. Our research aimed to assess the genotypic performance of five blueberry cultivars, including ‘Echota’, ‘O’Neal’, ‘Reveille’, ‘Summit’, and ‘Sunrise’. The selected cultivars were phenotyped for various fruit quality-related traits over two sequential harvests in two years and two locations. Our results indicated that genotype was a significant source of variation for most phenotypic characteristics. Further, the effect of Y × H and G × Y × H significantly affected the majority of studied phenotypic traits. Within the studied genotypes, ‘Reveille’ and ‘O’Neal’ phenotypic stability were consistent across locations and years; additionally, ‘Summit’ phenotypic characteristics were stable across years, environments, and harvests. Clonal plant replicates within a genotype, harvest, and environment, in addition to individual fruit measures, were the most significant sources of variability.
The Impact of Cardiovascular Rehabilitation on Psychophysiological Stress, Personality and Tryptophan Metabolism: A Randomized Pilot Feasibility Study
Authors: Wagner-Skacel Jolana , Mörkl Sabrina , Dalkner Nina , Fellendorf Frederike , Fitz Werner , Brix Bianca , Neshev Ruslan , Wedenig Sarah , Mächler Petra , Dorr Andreas , Picha Rainer , Rudlof Maximilian E. , Bartel Till O. , Tatschl Josef M. , Gostner Johanna M. , Bengesser Susanne A. , Reininghaus Eva Z. , Jenewein Josef , Goswami Nandu
Published: September 2021

Multicomponent cardiac rehabilitation (CR) is a secondary prevention strategy for cardiac patients to tackle stress and psychosocial wellbeing. However, there is a lack of data on its psychoneuroimmunological effects and of biomarkers to determine individual risk and to develop treatment strategies. We conducted a pilot randomized controlled trial (RCT) to investigate the feasibility of deriving psychophysiological stress markers in patients with cardiovascular diseases. Thirty individuals with cardiovascular disease (mean age 58.8 years; 23.3% female) were enrolled and randomized into three treatment groups: standard rehabilitation, yoga, or transcendental meditation (TM). Depression, anxiety, sleep, stress perception, personality functioning, hair cortisol, serum tryptophan, kynurenine and neopterin concentrations were estimated at baseline and after a four-week intervention. Hair cortisol levels decreased significantly after rehabilitation in all groups (F = 15.98, p < 0.001). In addition, personality functioning improved in all patients over time. Participants with impairments in personality functioning showed a positive correlation with baseline neopterin that did not remain significant after Bonferroni correction. Concentrations of serum tryptophan and its metabolite kynurenine did not change significantly. This pilot RCT provides preliminary evidence of multicomponent CR leading to stabilization of hair cortisol levels and improved psychophysiological wellbeing and personality functioning. Impairments in personality functioning were correlated with neopterin levels, which may impact the symptomatology and outcome.
Gut Microbiota Has a Crucial Role in the Development of Hypertension and Vascular Dysfunction in Toll-like Receptor 7-Driven Lupus Autoimmunity
Authors: de la Visitación Néstor , Robles-Vera Iñaki , Moleón Javier , González-Correa Cristina , Aguilera-Sánchez Nazaret , Toral Marta , Gómez-Guzmán Manuel , Sánchez Manuel , Jiménez Rosario , Martin-Morales Natividad , O’Valle Francisco , Romero Miguel , Duarte Juan
Published: September 2021

Our group has investigated the involvement of gut microbiota in hypertension in a murine model of systemic lupus erythematosus induced by Toll-like receptor (TLR)-7 activation. Female BALB/c mice were randomly assigned to four experimental groups: an untreated control (CTR), a group treated with the TLR7 agonist imiquimod (IMQ), IMQ-treated with vancomycin, and IMQ-treated with a cocktail of broad-spectrum antibiotics. We carried out faecal microbiota transplant (FMT) from donor CTR or IMQ mice to recipient IMQ or CTR animals, respectively. Vancomycin inhibited the increase in blood pressure; improved kidney injury, endothelial function, and oxidative stress; and reduced T helper (Th)17 infiltration in aortas from IMQ-treated mice. The rise in blood pressure and vascular complications present in IMQ mice were also observed in the CTR mice recipients of IMQ microbiota. Reduced relative populations of Sutterella and Anaerovibrio were associated with high blood pressure in our animals, which were increased after stool transplantation of healthy microbiota to IMQ mice. The reduced endothelium-dependent vasodilator responses to acetylcholine induced by IMQ microbiota were normalized after interleukin-17 neutralization. In conclusion, gut microbiota plays a role in the TLR7-driven increase in Th17 cell, endothelial dysfunction, vascular inflammation, and hypertension. The vascular changes induced by IMQ microbiota were initiated by Th17 infiltrating the vasculature.
Selenoprotein T Protects Endothelial Cells against Lipopolysaccharide-Induced Activation and Apoptosis
Authors: Merk Dennis , Ptok Johannes , Jakobs Philipp , von Ameln Florian , Greulich Jan , Kluge Pia , Semperowitsch Kathrin , Eckermann Olaf , Schaal Heiner , Ale-Agha Niloofar , Altschmied Joachim , Haendeler Judith
Published: September 2021

Sepsis is an exaggerated immune response upon infection with lipopolysaccharide (LPS) as the main causative agent. LPS-induced activation and apoptosis of endothelial cells (EC) can lead to organ dysfunction and finally organ failure. We previously demonstrated that the first twenty amino acids of the Apurinic/Apyrimidinic Endodeoxyribonuclease 1 (APEX1) are sufficient to inhibit EC apoptosis. To identify genes whose regulation by LPS is affected by this N-terminal APEX1 peptide, EC were transduced with an expression vector for the APEX1 peptide or an empty control vector and treated with LPS. Following RNA deep sequencing, genes upregulated in LPS-treated EC expressing the APEX1 peptide were identified bioinformatically. Selected candidates were validated by semi-quantitative real time PCR, a promising one was Selenoprotein T (SELENOT). For functional analyses, an expression vector for SELENOT was generated. To study the effect of SELENOT expression on LPS-induced EC activation and apoptosis, the SELENOT vector was transfected in EC. Immunostaining showed that SELENOT was expressed and localized in the ER. EC transfected with the SELENOT plasmid showed no activation and reduced apoptosis induced by LPS. SELENOT as well as APEX1(1-20) can protect EC against activation and apoptosis and could provide new therapeutic approaches in the treatment of sepsis.
Autoxidation Enhances Anti-Amyloid Potential of Flavone Derivatives
Authors: Sakalauskas Andrius , Ziaunys Mantas , Snieckute Ruta , Smirnovas Vytautas
Published: September 2021

The increasing prevalence of amyloid-related disorders, such as Alzheimer’s or Parkinson’s disease, raises the need for effective anti-amyloid drugs. It has been shown on numerous occasions that flavones, a group of naturally occurring anti-oxidants, can impact the aggregation process of several amyloidogenic proteins and peptides, including amyloid-beta. Due to flavone autoxidation at neutral pH, it is uncertain if the effective inhibitor is the initial molecule or a product of this reaction, as many anti-amyloid assays attempt to mimic physiological conditions. In this work, we examine the aggregation-inhibiting properties of flavones before and after they are oxidized. The oxidation of flavones was monitored by measuring the UV-vis absorbance spectrum change over time. The protein aggregation kinetics were followed by measuring the amyloidophilic dye thioflavin-T (ThT) fluorescence intensity change. Atomic force microscopy was employed to image the aggregates formed with the most prominent inhibitors. We demonstrate that flavones, which undergo autoxidation, have a far greater potency at inhibiting the aggregation of both the disease-related amyloid-beta, as well as a model amyloidogenic protein—insulin. Oxidized 6,2′,3′-trihydroxyflavone was the most potent inhibitor affecting both insulin (7-fold inhibition) and amyloid-beta (2-fold inhibition). We also show that this tendency to autoxidize is related to the positions of the flavone hydroxyl groups.
The Study of Corrosion Behaviors of Carbon Steel Weldments and Their Inhibition in Simulated Pore Solution Using Multi-Electrode Array Technique
Authors: Yang Lujia , Ma Zhenping , Zheng Yufeng , Wang Xiaona , Huang Yi , Wang Kangchen , Song Shenyou , Jin Wenliang
Published: September 2021

A multi-electrode array sensor was developed to study the corrosion behaviors of carbon steel weldments and the effectiveness of the NaNO2 inhibitor in carbonated pore solution. The sensor can simulate a complete weldment, and the measurement results can match well with the coupon immersion test. The galvanic corrosion between the weld area, heat-affected area, and base metal area, as well as the effect of nitrite corrosion inhibitor on the weld area, were observed by measuring the open circuit potential, coupling potential, and galvanic current. The results show that corrosion is likely to happen around the weld metal area and its adjacent heat-affected zone. The intensive galvanic currents can accelerate the localized corrosion, while NaNO2 can inhibit it.
Multi-RAT Orchestration Method for Heterogeneous Wireless Networks
Authors: Więcek Dariusz , Michalski Igor , Rzeźniczak Krzysztof , Wypiór Dariusz
Published: September 2021

Currently, energy efficiency (EE) of wireless communication is essential where many wireless networks with different Radio Access Technologies (RATs) coexist together. The RATs can be effectively selected and managed on a higher level to achieve maximum EE and save energy, e.g., save batteries. The approach to wireless traffic steering in mobile networks with a proof-of-concept solution is presented in this paper, owing to the developed high-level multi-RAT (multi-Radio Access Technology) heterogonous network orchestration approach. Based on the high-level network orchestrator, which traces network indicators, it is possible to decrease the user mobile terminal energy consumption, keeping traffic speed at an adequate level. The solution discussed was implemented in an experimental testbed with Software Defined Radio transmission systems. Downlink and uplink data links were toggled among different RATs according to the decisions that were taken by the end-to-end multi-RAT orchestrator based on the received proper network traffic-related indicators. The authors focused on finding an adequate algorithm that allowed for reduced power usage in the user terminal and made the attempt to verify how to reach the power reduction without introducing RAT-specific rules. The results showed that the proposed orchestration EE reduction was observed (from 11% to 42% for two different scenarios) in relation to the single LTE network deployment. The orchestration compared to the Wi-Fi network does not provide EE gain (−7% and 0%, respectively), but allows the user to achieve a higher data rate (23% and 39% gain, respectively), thus keeping the energy efficiency at almost the same level.
Pain Assessment during Gnathological Treatment of Temporomandibular Myofascial Pain through Mandibular Repositioning Splints Designed after a Posture-Stabilometric Evaluation
Authors: D’Attilio Michele , Migliore Federica , Moscagiuri Francesco , Caroccia Francesco
Published: September 2021

The aim of the study is to evaluate the effectiveness of two complementary mandibular repositioning splints (SVED and MORA) designed after a preliminary patients’ posture-stabilometric evaluation in treatment for temporomandibular myofascial pain (TMP) using the Pain-Intensity Numeric Rating Scale (PI-NRS) assessment. Eighty-six consecutive sportive non-agonistic subjects with TMP were randomly divided in two groups. Forty-two wear mandibular repositioning splints designed by a posture-stabilometric evaluation, thus constituting the test group. The other 44 subjects were not subjected to any treatment, thus represented the control group. To record pain intensity, subjects in both groups were asked to fill in a PI-NRS which ranged from 0 (no pain) to 10 (worst imaginable pain). In the test group, PI-NRS was assessed three times: before starting therapy (t0), after 4 months (t1) and after 8 months (t2) of treatment. Instead, in the control group PI-NRS was recorded during the first visit (t0) and after 8 months (t2). The Friedman test showed a statistically significant decrease in PI-NRS mean scores after 4 (t1) and 8 (t2) months from the start of the gnathological therapy with mandibular repositioning splints, (p < 0.001) in test group. There is enough evidence to assess that occlusal splint therapy for mandibular repositioning (MORA and SVED) designed through a posture-stabilometric evaluation could be considered in the treatment of temporomandibular myofascial pain.
Application of Machine Learning Methods for Pallet Loading Problem
Authors: Aylak Batin Latif , İnce Murat , Oral Okan , Süer Gürsel , Almasarwah Najat , Singh Manjeet , Salah Bashir
Published: September 2021

Because of continuous competition in the corporate industrial sector, numerous companies are always looking for strategies to ensure timely product delivery to survive against their competitors. For this reason, logistics play a significant role in the warehousing, shipments, and transportation of the products. Therefore, the high utilization of resources can improve the profit margins and reduce unnecessary storage or shipping costs. One significant issue in shipments is the Pallet Loading Problem (PLP) which can generally be solved by seeking to maximize the total number of boxes to be loaded on a pallet. In many previous studies, various solutions for the PLP have been suggested in the context of logistics and shipment delivery systems. In this paper, a novel two-phase approach is presented by utilizing a number of Machine Learning (ML) models to tackle the PLP. The dataset utilized in this study was obtained from the DHL supply chain system. According to the training and testing of various ML models, our results show that a very high (>85%) Pallet Utilization Volume (PUV) was obtained, and an accuracy of >89% was determined to predict an accurate loading arrangement of boxes on a suitable pallet. Furthermore, a comprehensive analysis of all the results on the basis of a comparison of several ML models is provided in order to show the efficacy of the proposed methodology.
Design and Trajectory Tracking Control of a Magnetorheological Prosthetic Knee Joint
Authors: Zuo Qiang , Zhao Jinpeng , Mei Xin , Yi Feng , Hu Guoliang
Published: September 2021

This paper developed a new magnetorheological (MR) prosthetic knee joint using an MR damper as the brake. According to the gait data of healthy people walking on flat ground, the structure of a MR prosthetic knee joint was expounded in detail, and its motion and dynamic model was also established. In addition, an MR damper was developed according to the specific needs of an MR prosthesis. The forward and reverse mechanical models of the MR damper were established, and its damping performance was obtained through experimental tests. In addition, to solve the problems of uncertainty and external interference in the MR prosthetic knee joint system, a second-order sliding mode controller was proposed. The experimental test results show the maximum positive error of the knee joint swing trajectory is 9.4°, which effectively tracks the reference swing trajectory.
Tunable Temperature Characteristic of Terahertz Bragg Fiber Filled with Liquid Water
Authors: Guo Cuijuan , Su Mengya , Shi Jia , Tian Linlin , Xu Wei , Chen Hongli , Bai Hua , Xiao Zhitao , Xu Degang , Yao Jianquan
Published: September 2021

Hollow-core terahertz (THz) fibers have attracted a lot of research interest due to the low loss and easy inner modification with functional materials. Liquid water has unique properties in the THz region and has been widely investigated in THz emission, sensing, and devices. In this paper, a hollow-core THz Bragg fiber with a water defect layer is proposed. The finite element method is used to verify and analyze the tunable temperature characteristic of the water-filled THz fiber. The numerical analysis results show that the confinement loss and the low-frequency side of the dip near 0.5 THz can be controlled by the temperature of the liquid water. The temperature sensitivity of the THz fiber is obtained at 0.09614 dB·m−1/K at 0.45 THz with a high core power fraction up to 98%. The proposed THz fiber has potential applications in THz interaction with liquid and THz tunable devices.
Chemometric Differentiation of Pistachios (Pistacia vera, Greek ‘Aegina’ Variety) from Two Different Harvest Years Using FTIR Spectroscopy and DRIFTS and Disk Techniques
Authors: Valasi Lydia , Pappas Christos S.
Published: September 2021

Food quality is a topic of utmost importance as more and more emphasis is placed on quality rather than quantity of products. Previous studies have pointed out the interaction of quality with the harvest year. In this study, 22 Pistacia vera (Greek ‘Aegina’ variety) samples (11 from 2017 and 11 from 2018) were differentiated using Fourier transform infrared spectroscopy (FTIR) and (a) diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and (b) KBr/sample disk techniques. In both years, the pistachios trees’ growing followed standard cultivation methods and similar agronomic conditions. Two chemometric models were developed using partial least squares-discrimination analysis (PLS-DA). DRIFTS proved unable to statistically differentiate the samples (R2 = 0.96266, Q2 = 0.63152). On the contrary, the disk technique completely differentiated the pistachio samples (R2 = 0.99705, Q2 = 0.97719). The 1720–1800 cm−1 region mostly contributed to the discrimination. The disk-FTIR chemometric model is fast, robust, economical, and environmentally friendly for determining pistachio matrix quality.
Robust Controller Decorated by Nonlinear S Function and Its Application to Water Tank
Authors: Zhang Xianku , Song Chunyu
Published: September 2021

In this manuscript, a concept of modifying the results of the existing robust controller decorated by a nonlinear S function is presented to improve the system performance. A case-based study of level control of water tanks illustrates the effectiveness of nonlinear decoration in improving robustness and controlling energy-saving performance with an S-function-decorated robust controller. The performance of the controlled system was analyzed through Lyapunov stability theorem and robust control theory, and was evaluated with a performance index. By demonstrating three comparing simulations of different scenes, it testifies to the fact that the nonlinear decorated robust controller meets the requirement of improving the system performance index. Compared with the nonlinear feedback and the fuzzy control, the performance index of the system using a nonlinear decorated controller is reduced by more than 10% with satisfactory robustness. This nonlinear decorated robust controller is proven to be energy efficient, simple and clear and easy to use, valuable for extensive application.
AimR Adopts Preexisting Dimer Conformations for Specific Target Recognition in Lysis-Lysogeny Decisions of Bacillus Phage phi3T
Authors: Pei Kai , Zhang Jie , Zou Tingting , Liu Zhu
Published: September 2021

A bacteriophage switches between lytic and lysogenic life cycles. The AimR-AimP-AimX communication system is responsible for phage lysis-lysogeny decisions during the infection of Bacillus subtilis. AimX is a regulator biasing phage lysis, AimR is a transcription factor activating AimX expression, and AimP is an arbitrium peptide that determines phage lysogeny by deactivating AimR. A strain-specific mechanism for the lysis-lysogeny decisions is proposed in SPbeta and phi3T phages. That is, the arbitrium peptide of the SPbeta phage stabilizes the SPbeta AimR (spAimR) dimer, whereas the phi3T-derived peptide disassembles the phi3T AimR (phAimR) dimer into a monomer. Here, we find that phAimR does not undergo dimer-to-monomer conversion upon arbitrium peptide binding. Gel-filtration, static light scattering (SLS) and analytical ultracentrifugation (AUC) results show that phAimR is dimeric regardless of the presence of arbitrium peptide. Small-angle X-ray scattering (SAXS) reveals that the arbitrium peptide binding makes an extended dimeric conformation. Single-molecule fluorescence resonance energy transfer (smFRET) analysis reveals that the phAimR dimer fluctuates among two distinct conformational states, and each preexisting state is selectively recognized by the arbitrium peptide or the target DNA, respectively. Collectively, our biophysical characterization of the phAimR dynamics underlying specific target recognition provides new mechanistic insights into understanding lysis-lysogeny decisions in Bacillus phage phi3T.
The Effectiveness of Influenza Vaccination on Chronic Obstructive Pulmonary Disease with Different Severities of Airflow Obstruction
Authors: Chang Hui-Chuan , Liu Shih-Feng , Li Ying-Chun , Kuo Ho-Chang , Tsai Yun-Chyn , Chen Min-Hui
Published: September 2021

This retrospective study included COPD patients who attended our medical center between January and October 2018, and analyzed the outcomes of their influenza vaccination, including medical visits, hospitalization, medical expenses, and the incidence of respiratory failure. Airflow limitation was stratified according to GOLD guidelines. Overall, 543 COPD patients were enrolled, including 197, 113, 126, and 107 mild, moderate, severe, and very severe patients, respectively. Of all the participants, 238 received an influenza vaccination (43.8%), which significantly reduced hospital utilization for moderate (odds ratio [OR] 0.22, 95%CI 0.09–0.51), severe (OR 0.19, 95%CI 0.08–0.44), and very severe patients (OR 0.15, 95%CI 0.05–0.5) compared to mild patients (OR 0.51, 95%CI 0.2–1.26); reduced emergency department utilization for moderate (OR 0.33, 95%CI 0.14–0.77), severe (OR 0.22, 95%CI 0.10–0.52), and very severe patients (OR 0.30, 95%CI 0.10–0.88) compared to mild patients (OR 0.64, 95%CI 0.30–1.37); and reduced the occurrence of respiratory failure for moderate (OR 0.20, 95%CI 0.06–0.68), severe (OR 0.40, 95%CI 0.16–0.98), and very severe patients (OR 0.36, 95%CI 0.15–0.82) compared to mild patients (OR 0% CI 0.14–3.20). Influenza vaccination is more effective in COPD patients with moderate, severe, and very severe airflow obstruction than in those with mild obstruction with respect to hospital utilization, emergency department utilization, and respiratory failure.
Dietary Supplementation of Antioxidant Compounds Prevents Light-Induced Retinal Damage in a Rat Model
Authors: Amato Rosario , Canovai Alessio , Melecchi Alberto , Pezzino Salvatore , Corsaro Roberta , Dal Monte Massimo , Rusciano Dario , Bagnoli Paola , Cammalleri Maurizio
Published: September 2021

Light-induced retinal damage (LD) is characterized by the accumulation of reactive oxygen species leading to oxidative stress and photoreceptor cell death. The use of natural antioxidants has emerged as promising approach for the prevention of LD. Among them, lutein and cyanidin-3-glucoside (C3G) have been shown to be particularly effective due to their antioxidant and anti-inflammatory activity. However, less is known about the possible efficacy of combining them in a multicomponent mixture. In a rat model of LD, Western blot analysis, immunohistochemistry and electroretinography were used to demonstrate that lutein and C3G in combination or in a multicomponent mixture can prevent oxidative stress, inflammation, gliotic and apoptotic responses thus protecting photoreceptor cells from death with higher efficacy than each component alone. Combined efficacy on dysfunctional electroretinogram was also demonstrated by ameliorated rod and cone photoreceptor responses. These findings suggest the rationale to formulate multicomponent blends which may optimize the partnering compounds bioactivity and bioavailability.
Democratic Administration and Commitment of Members of Agricultural Cooperatives: A Case Study from a Prefecture in Greece
Authors: Ragazou Konstantina , Anastasiou Evgenia , Theodossiou George , Koutsogeorgos Konstantinos
Published: September 2021

Cooperatives are democratic organizations, governed and controlled by their members, who are actively involved in their policy-making and decision-making process. The aim of this paper is to investigate the correlation between cooperative culture and the way that cooperatives are governed. To this end, a probability sampling method is used in the agricultural cooperatives of the Greek prefecture of Larissa, which is one of the most powerful and dynamic in the agricultural economy. The data collection was carried out to 100 members of agricultural cooperatives through the use of a closed-ended questionnaire. The findings highlighted that agricultural cooperatives are distinguished for their increased level of cooperative culture and commitment, provided that the conditions for the democratic governance of cooperatives are met. The role played by the level of education of the members of the agricultural cooperatives was also important, thus confirming the main purpose of the research, which was none other than to prove this correlation. Finally, this correlation can lead to the improvement of certain elements which contribute to the optimization of agricultural governance.
Magnetic Aromaticity of Cycloporphyrin Nanorings
Authors: Landi Alessandro , Summa Francesco Ferdinando , Monaco Guglielmo
Published: September 2021

The ascertainment of magnetic aromaticity is not necessarily straightforward, especially for large and bent systems, such as the cycloporphyrin nanorings recently synthesized by the group of Anderson. Six of these cycloporphyrin nanorings were studied here computationally. Indirect methods, based on nuclear shielding and magnetizabilities, and direct methods, based on standard quantum mechanics, were both used effectively to determine their magnetically induced current strength, which mostly confirmed Anderson’s classification. However, in the case of hexanions, and in particular for cyclohexaporphyrin hexacations, a significant cancellation of delocalized diatropic and paratropic flow occurred, showing that the resultant faint aromatic character was a result of competing aromatic and antiaromatic contributions, as also evidenced by the ipsocentric method. A warning is renewed on the use of isotropic shielding to determine the tropicity of the magnetically induced current.