bluuebunny/crossref_metadata_2025 huggingface dataset:
Hyperspectral cathodoluminescence, trace element, and U-Pb geochronological characterization of apatite from the Ernest Henry iron oxide copper-gold (IOCG) deposit, Cloncurry district, Queensland
Authors: Steadman Jeffrey A. , Goemann Karsten , Thompson Jay M. , MacRae Colin M. , Belousov Ivan , Hohl Max
Published: October 2022

Hyperspectral cathodoluminescence (CL), geochemical, and geochronological characterization of a series of apatite-bearing samples from within and around the Ernest Henry IOCG deposit, NW Queensland, Australia, have revealed complex mineral parageneses and a spectrum of U-Pb ages that point to the effects of multiple geological processes. No two samples are identical, either in geochemistry or texture, despite their relative proximity to one another (all samples within 5 km from Ernest Henry). Hyperspectral CL maps reveal diverse internal textures and emissions ranging from near infrared (NIR) to near ultraviolet (UV) with a complex series of spectra in all samples, requiring the fitting more than 40 individual peaks (both sharp and broad) to capture the observed variability. Imaging analyses via LA-ICPMS show that apatite from the Ernest Henry district is enriched above background in a variety of trace elements, including Na, Mg, Al, Si, V, Mn, As, Sr, Y, the rare Earth elements (REEs), Pb, Th, and U. Samples outside the ore zone display chondrite-normalized REE profiles that are consistent with either a magmatic or hydrothermal origin, whereas ore zone apatite exhibits profiles that are decidedly hydrothermal in nature. Moreover, specific zones within ore zone apatite grains are very As-rich (up to 7 wt% As2O5), and the effect of such high As on the hyperspectral CL signature of these zones is a pronounced dampening of CL emission, regardless of REE concentrations. Uranium-Pb dating of the same samples (via LA-ICPMS) has yielded a diverse array of overlapping Mesoproterozoic ages ranging from 1,580 ± 34 Ma to 1,533 ± 61 Ma. These results correlate to published ages that constrain hydrothermal alteration in the Ernest Henry area, both before and during Cu-Au mineralization. Collectively, these data highlight the complexity of apatite studies at Ernest Henry, the broader Cloncurry district, and probably analogous terranes elsewhere. A combination of micro-scale methods such as those used in this study are shown to be essential for accurately deciphering geological information contained within petrogenetic indicator minerals.
Curriculum development for student agency on sustainability issues: An exploratory study
Authors: Rap Shelley , Blonder Ron , Sindiani-Bsoul Ayshi , Rosenfeld Sherman
Published: October 2022

Despite unprecedented global challenges to the environment, research show that many young people are pessimistic about their ability to address these challenges. This paper explores one approach designed to guide middle-school teachers and their students to develop and practice agency about sustainability issues: via a curriculum that challenges students to solve problems by analyzing real-world data and developing scientific arguments, as a basis for engaging in activism. The paper begins with an overview of the United Nation’s Agenda 2030, its Sustainable Development Goals (SDGs), the related aims of Education for Sustainable Development (ESD), and a review of what is meant by student agency. Next, the goals and design features of a curricular initiative, “Speak to Me in Numbers,” are presented with a brief presentation of two units, each based on a different SDG. The paper’s research questions are (1) How were the design features of the curriculum perceived by the teachers? and (2) What were the preliminary outcomes of the curriculum in terms of student and teacher argumentation skills and student activism? To address these questions, we present an exploratory study: observations and comments from in-service teachers and participating students regarding preliminary outcomes of the curriculum that might be related to the development of student agency. In our concluding discussion, based on these findings and relevant literature, we suggest that a promising pedagogy to strengthen student agency on sustainability issues is a data-driven pedagogy that focuses on the development of scientific argumentation, mathematical thinking and activism.
Increased levels of serum adenosine deaminase and increased risk of diabetic peripheral neuropathy in type 2 diabetes
Authors: Yu Chao , Zhuang Lei , Xu Feng , Zhao Li-hua , Wang Xiao-hua , Wang Chun-hua , Ning Li-yan , Zhang Xiu-lin , Zhang Dong-mei , Wang Xue-qin , Su Jian-bin
Published: October 2022

Background

Increased serum adenosine deaminase (ADA) levels have been shown to be involved in metabolic abnormalities and immune disequilibrium, which may in turn contribute to inflammatory diseases. This study aimed to determine whether increased serum ADA levels are related to diabetic peripheral neuropathy (DPN) in patients with type 2 diabetes (T2D).

Methods

This study was part of a series exploring the potential risks for DPN. All patients received DPN assessment based on neuropathic symptoms, neuropathic signs, and nerve conduction studies to calculate the compositeZscore of nerve latency, amplitude and conduction velocity (NCV). DPN was confirmed by both at least a presentation of neuropathic symptoms/signs and an abnormal nerve conduction index. Serum ADA levels were also synchronously detected.

Results

A total of 384 eligible patients with T2D were recruited for this study, and 24.5% (n=94) were determined to have DPN. Increases in serum ADA levels were closely associated with increases in compositeZscore of latency (β=0.263,t=5.273,p<0.001) and decreases in compositeZscore of amplitude (β=–0.126,t=–2.352,p=0.019) and NCV (β=–0.201,t=–3.841,p<0.001) after adjusting for other clinical covariates. Moreover, each 5 U/L increase in serum ADA levels was associated with a 1.781-fold increased adjusted odds ratio of having DPN (95% confidence interval: 1.271–2.495). Furthermore, the optimal cut-off value of serum ADA levels to discriminate DPN was ≥14.2 U/L (sensitivity=59.57%, specificity=75.52% and Youden index=0.351) after analysis by receiver operating characteristic curve.

Conclusions

Increased serum ADA levels may be a potential risk factor for DPN in patients with T2D.
Prevalence of arsenic contamination in rice and the potential health risks to the Bahamian population—A preliminary study
Authors: Watson Carlton , Gustave Williamson
Published: October 2022

Rice is among the most important staple foods worldwide. However, the consumption of rice and rice-based food products poses a potential health risk since rice is a paddy crop that is well known to accumulate high concentrations of arsenic (As) in its grain. In The Bahamas, although rice is heavily consumed, it is not grown locally. Instead, all the consumed rice and its derived products are imported. Recent food surveys in the major rice exporting countries have shown that a significant portion of their market rice products is contaminated with As. However, to date, the prevalence of As in the rice foods available in The Bahamas remains unknown. Therefore, in this study, we surveyed the occurrence of As in a selection of rice and rice products that were on sale in the Bahamian market. A total of 21 different rice brands were collected. The concentration of As and the potential health risk were estimated by target hazard quotient (THQ), hazard index (HI), and lifetime cancer risk (LCR). Our results showed that only the blue ribbon samples had an estimated inorganic arsenic (iAs) concentration above the World Health Organization (WHO) safety limits (200 μg/kg), which is based on global average consumption. However, when we factor for average rice consumption in The Bahamas, 79% of the rice samples had iAs concentration values indicative of carcinogenic risks and 57% had iAs concentration values that suggested non-carcinogenic health risks. Based on our results, we recommend urgent follow-up studies to further test rice varieties that show the greatest LCR and HI values and to also broaden the study to include more off-brand/generic varieties, cooked rice, and drinking water.
Corporate social responsibility regulation in capital market and environmental information disclosure of listed companies: A quasi-natural experiment from China
Authors: Zhang Changjiang , Zhang Yue , Zhang Sihan , Hou Mengxiao , Chen Yuqing
Published: October 2022

Based on a quasi-natural experiment generated by the Shenzhen Stock Exchange (SZSE) of China, which issued the Guidance for Social Responsibility of Listed Companies (referred to as Guidance) in 2006, this paper utilizes a panel dataset of A-share listed companies at Shanghai Stock Exchange (SSE) and SZSE from 2004 to 2008, and employ difference-in-differences (DID) method to investigate impact of the Guidance on environmental information disclosure quality (Eidq) of listed companies. The finding shows that exchange’s corporate social responsibility (CSR) regulation contributes to improving the Eidq of listed companies. Furthermore, policy effects of the Guidance are more significant in eastern region, heavily polluting industries and state-owned enterprises (SOEs). This study provides theoretical evidence and policy implications for the “two-wheel drive” of China’s stock market regulation and social supervision, and for the construction of an environmental information disclosure system that is more targeted in terms of region, industry and property rights, and that effectively promotes fulfillment of environmental governance responsibility by listed companies and guides their sustainable development actions.
Dispersal and distribution of a generalist predator in habitats with multiple food resources
Authors: Sanchez Juan Antonio , Gillespie David R.
Published: October 2022

The ability to locate suitable food resources affects fitness in animals. Therefore, movements are necessary to optimize foraging in habitats where food is distributed in patches of different qualities. The aim of this work was to investigate the dispersal and distribution of females and males of the omnivorous mirid D. hesperus in mesocosms composed by food patches of different values in terms of fitness. In agreement with the Marginal Value Theorem (MVT) and the Ideal Free Distribution (IFD), individuals were expected to aggregate in the highest quality patches. Besides, the proportion of individuals in patches was predicted to be proportional to fitness, and interference among individuals was expected to rise as the density of individuals increased. Emigration rates were predicted to be higher for low- than for high-quality patches, while the opposite was predicted for immigration. Three types of habitats each with different combinations of food resources were tested: (1) habitat including patches of tomato plants with no-prey, and patches infested with either mite or whitefly; (2) with no-prey and whitefly; (3) with no-prey and mites. Each type of habitat was set up in a tomato greenhouse compartment and replicated four times. Individuals were tracked by mark-recapture methods using luminous paintings. The number of females and males in whitefly patches was significantly higher than in mite and no-prey patches, but a significant interaction sex*habitat and sex*patch was found. In habitats with only one type of prey, D. hesperus adults fitted the IFD, while in mixed prey habitats their distribution diverged from IFD. Interference was found to be significant, with female fitness decreasing as their density increased. Emigration rates were significantly lower for whitefly patches with a significant interaction patch*sex; the opposite was found for immigration. This research shows that it is unlikely that D. hesperus forage according to the omniscient principle of IFD and MVT; in contrast, it strongly suggests that it uses some simple rules to make decisions about inter-patch movement, and emigration from habitats and patches.
Relationship between brain size and digestive tract length support the expensive-tissue hypothesis in Feirana quadranus
Authors: Fu Yiping , Song Yanling , Yang Chao , Liu Xinyi , Liu Yenan , Huang Yan
Published: October 2022

The brain is among the most energetically costly organs in the vertebrate body, while the size of the brain varies within species. The expensive-tissue hypothesis (ETH) predicts that increasing the size of another costly organ, such as the gut, should compensate for the cost of a small brain. Here, the ETH was tested by analyzing the relationship between brain size variation and digestive tract length in a Swelled-vented frog (Feirana quadranus). A total of 125 individuals across 10 populations ranging from 586 to 1,702 m a.s.l. from the Qinling-Daba Mountains were sampled. With the increase in altitude, the brain size decreases and the digestive tract length increases. Different brain regions do not change their relative size in a consistent manner. The sizes of telencephalon and cerebellum decrease with the increase in altitude, while the olfactory nerve increases its size at high altitudes. However, the olfactory bulb and optic tectum have no significant relationship with altitude. After controlling for snout-vent length (SVL), a significant negative correlation could be found between brain size and digestive tract length in F. quadranus. Therefore, the intraspecific variation of brain size follows the general patterns of ETH in this species. The results suggest that annual mean temperature and annual precipitation are environmental factors influencing the adaptive evolution of brain size and digestive tract length. This study also suggests that food composition, activity times, and habitat complexity are the potential reasons driving the adaptive evolution of brain size and digestive tract length.
Structure‒function‒pathogenicity analysis of C-terminal myocilin missense variants based on experiments and 3D models
Authors: Zhou Biting , Lin Xiaojia , Li Zhong , Yao Yihua , Yang Juhua , Zhu Yihua
Published: October 2022

MYOC is a common pathogenic gene for primary open-angle glaucoma and encodes the protein named myocilin. Multiple MYOC variations have been found, with different clinical significance. However, the pathogenesis of glaucoma induced by MYOC mutations has not been fully clarified. Here, we analyze the molecular and cellular biological differences caused by multiple variant myocilins, including protein secretion characteristics, structural changes, subcellular localization, cellular autophagic activity and oxidative stress. Denaturing and nondenaturing electrophoresis showed myocilin to be a secreted protein with the tendency to self-oligomerize. The full-length myocilin and its C-terminal cleavage fragment are secreted. Secretion analysis of 23 variant myocilins indicated that secretion defects are closely related to the pathogenicity of MYOC variants. Structural analysis showed that the alteration of steric clash is associated with the secretion characteristics and pathogenicity of myocilin variants. Immunocytochemistry results demonstrated that mutated myocilins are retained in the endoplasmic reticulum and disrupt autophagy. MTT assay, MitoTracker staining, and DCFH-DA staining showed increased oxidative injury in cells expressing MYOC mutants. Taken together, MYOC mutations are able to induce cell dysfunction via secretion defects and intracellular accumulation resulting from steric clash alterations.
The prognostic impact of the immune signature in head and neck squamous cell carcinoma
Authors: Baysal Hasan , Siozopoulou Vasiliki , Zaryouh Hannah , Hermans Christophe , Lau Ho Wa , Lambrechts Hilde , Fransen Erik , De Pauw Ines , Jacobs Julie , Peeters Marc , Pauwels Patrick , Vermorken Jan Baptist , Smits Evelien , Lardon Filip , De Waele Jorrit , Wouters An
Published: October 2022

Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of tumors that retain their poor prognosis despite recent advances in their standard of care. As the involvement of the immune system against HNSCC development is well-recognized, characterization of the immune signature and the complex interplay between HNSCC and the immune system could lead to the identification of novel therapeutic targets that are required now more than ever. In this study, we investigated RNA sequencing data of 530 HNSCC patients from The Cancer Genome Atlas (TCGA) for which the immune composition (CIBERSORT) was defined by the relative fractions of 10 immune-cell types and expression data of 45 immune checkpoint ligands were quantified. This initial investigation was followed by immunohistochemical (IHC) staining for a curated selection of immune cell types and checkpoint ligands markers in tissue samples of 50 advanced stage HNSCC patients. The outcome of both analyses was correlated with clinicopathological parameters and patient overall survival. Our results indicated that HNSCC tumors are in close contact with both cytotoxic and immunosuppressive immune cells. TCGA data showed prognostic relevance of dendritic cells, M2 macrophages and neutrophils, while IHC analysis associated T cells and natural killer cells with better/worse prognostic outcome. HNSCC tumors in our TCGA cohort showed differential RNA over- and underexpression of 28 immune inhibitory and activating checkpoint ligands compared to healthy tissue. Of these, CD73, CD276 and CD155 gene expression were negative prognostic factors, while CD40L, CEACAM1 and Gal-9 expression were associated with significantly better outcomes. Our IHC analyses confirmed the relevance of CD155 and CD276 protein expression, and in addition PD-L1 expression, as independent negative prognostic factors, while HLA-E overexpression was associated with better outcomes. Lastly, the co-presence of both (i) CD155 positive cells with intratumoral NK cells; and (ii) PD-L1 expression with regulatory T cell infiltration may hold prognostic value for these cohorts. Based on our data, we propose that CD155 and CD276 are promising novel targets for HNSCC, possibly in combination with the current standard of care or novel immunotherapies to come.
The role of cancer-associated mesothelial cells in the progression and therapy of ovarian cancer
Authors: Zheng Aiping , Wei Yuhao , Zhao Yunuo , Zhang Tao , Ma Xuelei
Published: October 2022

Ovarian cancer is currently one of the most common malignant tumors in females with poor survival rates around the world, killing about 200,000 women each year. Although great progress has been made in treatment, most patients receiving first-line therapy experience tumor recurrence. The tumor microenvironment plays an important role in regulating the progression and prognosis of ovarian cancer. Cancer-associated mesothelial cells are the main cell population in the tumor microenvironment, which affect the progression, prognosis and chemical resistance of ovarian cancer. Cancer-associated mesothelial cells can also interact with other microenvironmental components, such as exosomes, macrophages, and adipocytes. Some studies have developed drugs targeting cancer-associated mesothelial cells in ovarian cancer to evaluate the therapeutic efficiency. In this review we highlighted the key role of cancer-associated mesothelial cells in the progression and prognosis of ovarian cancer. We also described the progress of cancer-associated mesothelial cells targeted therapy for ovarian cancer. Continued insight into the role of cancer-associated mesothelial cells in ovarian cancer will potentially contribute to the development of new and effective therapeutic regiments.
Dual immunological and proliferative regulation of immune checkpoint FGL1 in lung adenocarcinoma: The pivotal role of the YY1–FGL1–MYH9 axis
Authors: Tang Xi-Yang , Xiong Yan-Lu , Zhao Ya-Bo , Yang Jie , Shi An-Ping , Zheng Kai-Fu , Liu Yu-Jian , Shu Chen , Jiang Tao , Ma Nan , Zhao Jin-Bo
Published: October 2022

Rational

Lung cancer is the most common tumor worldwide, with the highest mortality rate and second highest incidence. Immunotherapy is one of the most important treatments for lung adenocarcinoma (LUAD); however, it has relatively low response rate and high incidence of adverse events. Herein, we explored the therapeutic potential of fibrinogen-like protein 1 (FGL1) for LUAD.

Methods

Data from GEPIA and ACLBI databases were assessed to explore gene–gene correlations and tumor immune infiltration patterns. A total of 200 patients with LUAD were recruited. FGL1 levels in the serum and cellular supernatant were determined by enzyme-linked immunosorbent assay. In vitro and in vivo experiments were performed to assess the effect FGL1 on the proliferation of LUAD cells. Cocultures were performed to explore the effect of FGL1 knockdown in lung cancer cells on T cells, concerning cytokine secretion and viability. PROMO and hTFtarget databases were used for transcription factor prediction. Quantitative polymerase chain reaction (qPCR), chromatin immunoprecipitation, and dual luciferase reporter assays were performed to validate the identified transcription factor of FGL1. Immunoprecipitation, mass spectrometry and gene ontology analysis were performed to explore the downstream partners of FGL1.

Results

FGL1 expression in LUAD was positively associated with PDL1, but not for PD1 expression. Moreover, FGL1 was positively associated with the CD3D expression and negatively associated with FOXP3, S100A9, and TPSB2 within the tumor site. FGL1 promotes the secretion of interleukin-2 by T cells in vitro, simultaneously inducing their apoptosis. Indeed, YY1 is the upstream molecule of FGL1 was found to be transcriptionally regulated by YY1 and to directly by to MYH9 to promote the proliferation of LUAD cells in vitro and in vivo.

Conclusions

FGL1 is involved in the immunological and proliferative regulation of LUAD cells by controlling the secretion of important immune-related cytokines via the YY1–FGL1–MYH9 axis. Hence, targeting FGL1 in LUAD may pave the way for the development of new immunotherapies for tackling this malignancy.
CD38+CD39+ NK cells associate with HIV disease progression and negatively regulate T cell proliferation
Authors: Qian Shi , Xiong Chunbin , Wang Meiting , Zhang Zining , Fu Yajing , Hu Qinghai , Ding Haibo , Han Xiaoxu , Shang Hong , Jiang Yongjun
Published: October 2022

The ectonucleotidases CD38 and CD39 have a critical regulatory effect on tumors and viral infections via the adenosine axis. Natural killer (NK) cells produce cytokines, induce cytotoxic responses against viral infection, and acquire immunoregulatory properties. However, the roles of CD38 and CD39 expressed NK cells in HIV disease require elucidation. Our study showed that the proportions of CD38+CD39+ NK cells in HIV-infected individuals were positively associated with HIV viral loads and negatively associated with the CD4+ T cell count. Furthermore, CD38+CD39+ NK cells expressed additional inhibitory receptors, TIM-3 and LAG-3, and produced more TGF-β. Moreover, autologous NK cells suppressed the proliferation of CD8+ T and CD4+ T cells of HIV-infected individuals, and inhibiting CD38 and CD39 on NK cells restored CD8+ T and CD4+ T cell proliferation in vitro. In conclusion, these data support a critical role for CD38 and CD39 on NK cells in HIV infection and targeting CD38 and CD39 on NK cells may be a potential therapeutic strategy against HIV infection.
Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis
Authors: Kelly Jenna N. , Laloli Laura , V’kovski Philip , Holwerda Melle , Portmann Jasmine , Thiel Volker , Dijkman Ronald
Published: October 2022

The respiratory epithelium constitutes the first line of defense against invading respiratory pathogens, such as the 2009 pandemic strain of influenza A virus (IAV, H1N1pdm09), and plays a crucial role in the host antiviral response to infection. Despite its importance, however, it remains unknown how individual cell types within the respiratory epithelium respond to IAV infection or how the latter may influence IAV disease progression and pathogenesis. Here, we used single cell RNA sequencing (scRNA-seq) to dissect the host response to IAV infection in its natural target cells. scRNA-seq was performed on human airway epithelial cell (hAEC) cultures infected with either wild-type pandemic IAV (WT) or with a mutant version of IAV (NS1R38A) that induced a robust innate immune response. We then characterized both the host and viral transcriptomes of more than 19,000 single cells across the 5 major cell types populating the human respiratory epithelium. For all cell types, we observed a wide spectrum of viral burden among single infected cells and a disparate host response between infected and bystander populations. Interestingly, we also identified multiple key differences in the host response to IAV among individual cell types, including high levels of pro-inflammatory cytokines and chemokines in secretory and basal cells and an important role for luminal cells in sensing and restricting incoming virus. Multiple infected cell types were shown to upregulate interferons (IFN), with type III IFNs clearly dominating the antiviral response. Transcriptional changes in genes related to cell differentiation, cell migration, and tissue repair were also identified. Strikingly, we also detected a shift in viral host cell tropism from non-ciliated cells to ciliated cells at later stages of infection and observed major changes in the cellular composition. Microscopic analysis of both WT and NS1R38A virus-infected hAECs at various stages of IAV infection revealed that the transcriptional changes we observed at 18 hpi were likely driving the downstream histopathological alterations in the airway epithelium. To our knowledge, this is the first study to provide a comprehensive analysis of the cell type-specific host antiviral response to influenza virus infection in its natural target cells – namely, the human respiratory epithelium.
Evidence for the heterologous benefits of prior BCG vaccination on COVISHIELD™ vaccine-induced immune responses in SARS-CoV-2 seronegative young Indian adults
Authors: Rakshit Srabanti , Adiga Vasista , Ahmed Asma , Parthiban Chaitra , Chetan Kumar Nirutha , Dwarkanath Pratibha , Shivalingaiah Sudarshan , Rao Srishti , D’Souza George , Dias Mary , Maguire Thomas J. A. , Doores Katie J. , Zoodsma Martijn , Geckin Busranur , Dasgupta Prokar , Babji Sudhir , van Meijgaarden Krista E. , Joosten Simone A. , Ottenhoff Tom H. M. , Li Yang , Netea Mihai G. , Stuart Kenneth D. , De Rosa Stephen C. , McElrath M. Juliana , Vyakarnam Annapurna
Published: October 2022

This proof-of-concept study tested if prior BCG revaccination can qualitatively and quantitively enhance antibody and T-cell responses induced by Oxford/AstraZeneca ChAdOx1nCoV-19 or COVISHIELD™, an efficacious and the most widely distributed vaccine in India. We compared COVISHIELD™ induced longitudinal immune responses in 21 BCG re-vaccinees (BCG-RV) and 13 BCG-non-revaccinees (BCG-NRV), all of whom were BCG vaccinated at birth; latent tuberculosis negative and SARS-CoV-2 seronegative prior to COVISHIELD™ vaccination. Compared to BCG-NRV, BCG-RV displayed significantly higher and persistent spike-specific neutralizing (n) Ab titers and polyfunctional CD4+ and CD8+ T-cells for eight months post COVISHIELD™ booster, including distinct CD4+IFN-γ+ and CD4+IFN-γ- effector memory (EM) subsets co-expressing IL-2, TNF-α and activation induced markers (AIM) CD154/CD137 as well as CD8+IFN-γ+ EM,TEMRA (T cell EM expressing RA) subset combinations co-expressing TNF-α and AIM CD137/CD69. Additionally, elevated nAb and T-cell responses to the Delta mutant in BCG-RV highlighted greater immune response breadth. Mechanistically, these BCG adjuvant effects were associated with elevated markers of trained immunity, including higher IL-1β and TNF-α expression in CD14+HLA-DR+monocytes and changes in chromatin accessibility highlighting BCG-induced epigenetic changes. This study provides first in-depth analysis of both antibody and memory T-cell responses induced by COVISHIELD™ in SARS-CoV-2 seronegative young adults in India with strong evidence of a BCG-induced booster effect and therefore a rational basis to validate BCG, a low-cost and globally available vaccine, as an adjuvant to enhance heterologous adaptive immune responses to current and emerging COVID-19 vaccines.
Protecting ocean carbon through biodiversity and climate governance
Authors: Elsler Laura G. , Oostdijk Maartje , Levin Lisa A. , Satterthwaite Erin V. , Pinsky Malin L. , Crespo Guillermo Ortuño , Wisz Mary S.
Published: October 2022

Global policy goals for halting biodiversity loss and climate change depend on each other to be successful. Marine biodiversity and climate change are intertwined through foodwebs that cycle and transport carbon and contribute to carbon sequestration. Yet, biodiversity conservation and fisheries management seldom explicitly include ocean carbon transport and sequestration. In order to effectively manage and govern human activities that affect carbon cycling and sequestration, international biodiversity and climate agreements need to address both biodiversity and climate issues. International agreements that address issues for climate and biodiversity are best poised to facilitate the protection of ocean carbon with existing policies. The degree to which the main international biodiversity and climate agreements make reference to multiple issues has however not been documented. Here, we used a text mining analysis of over 2,700 binding and non-binding policy documents from ten global ocean-related agreements to identify keywords related to biodiversity, climate, and ocean carbon. While climate references were mostly siloed within climate agreements, biodiversity references were included in most agreements. Further, we found that six percent of policy documents (n=166) included ocean carbon keywords. In light of our results, we highlight opportunities to strengthen the protection of ocean carbon in upcoming negotiations of international agreements, and via area-based management, environmental impact assessment and strategic environmental assessment.
Characterization of Pseudochloris wilhelmii potential for oil refinery wastewater remediation and valuable biomass cogeneration
Authors: Blažina Maria , Fafanđel Maja , Geček Sunčana , Haberle Ines , Klanjšček Jasminka , Hrustić Enis , Husinec Lana , Žilić Luka , Pritišanac Ena , Klanjscek Tin
Published: October 2022

The microalgae of the genus Pseudochloris/Picochlorum are characterized by fast growth, and wide nutrient (type and concentration) and salinity tolerance, all contributing towards exploration of their use in high-density biomass production and wastewater bioremediation. In this study, removal of nitrogen and phosphorus nutrients from oil refinery wastewater was monitored during growth of the marine eukaryotic microalgae Pseudochloris wilhelmii, with emphasis on biochemical analyses of its biomass quality to evaluate suitability for biodiesel production. A series of growth experiments under various nutrient and light regimes were performed in a temperature range of 20-30°C to evaluate nutrient removal and biomass growth dependence on temperature. The highest removal rate of dissolved inorganic nitrogen reached under the given experimental conditions was 0.823 mmol/(gday) accompanied by the corresponding biomass productivity of 115.2 mg/(Lday). Depending on light and temperature, the final lipid concentration ranged 181.5 – 319.8 mg/L. Furthermore, increase in nutrient load decreased the maximum specific growth rate by 25%, and the maximum specific removal rate of the dissolved inorganic nitrogen by 19%, whereas the duration of bioremediation process was nearly doubled. In contrast, constant light exposure expedited the nitrogen removal, i.e. bioremediation process, by almost 40%, while supporting over three times higher biomass productivity and the highest maximum specific growth rate of 0.528 g/(gday). The conditions favoring the highest nitrogen removal and highest toxicity reduction in oil refinery wastewater are met at 24°C and 130 µmol phot/(m2s). The highest proportion of carbon-binding to the P. wilhelmii biomass was noticed under the same conditions, thus indicating them as the most favorable conditions for hydrocarbon removal as well as for CO2 sequestration. Pseudochloris wilhelmii therefore represents a promising candidate for oil refinery wastewater remediation and valuable biomass cogeneration on a large-scale.
Spatial variability of hypoxia and coupled physical-biogeochemical controls off the Changjiang (Yangtze River) Estuary in summer
Authors: Wei Qinsheng , Yuan Yongquan , Song Shuqun , Zhao Yuhang , Sun Junchuan , Li Caiwen , Wang Baodong
Published: October 2022

Global estuarine hypoxia is among the most acute environmental issues, yet the recognition of hypoxia variability remains patchy. The Changjiang (Yangtze River) Estuary (CE) is well known as one of the largest estuarine hypoxic systems. However, the mechanisms controlling the spatial variability and heterogeneity of summer hypoxia have been rarely examined in this region. Based on observational data in the summer of 2013, the essential linkages between physical-biogeochemical processes and spatial variability of hypoxia were revealed off the CE. The hypoxia generally occurred in a transitional region off the CE, presenting to be spatially variable and heterogeneous in its distribution and intensity. The river plume, stratification, front, upwelling and localized high phytoplankton biomass combine to shape the spatial morphology and scope of hypoxia. The spatial heterogeneity of hypoxic magnitude in horizontal direction is mainly regulated by the intensity variability of stratification and phytoplankton blooms. The position of bottom front formed by the offshore Taiwan Warm Current (TWC) and coastal water under tidal mixing controls the shoreward boundary of hypoxic zone, while the river plume front-dominated outer edge of phytoplankton blooms in upper layers plays a role in determining the seaward limit of the reach of bottom hypoxia. The stratification depth controlled by the upwelling and river plume governs the upper limit of the top reach of hypoxia, and shapes the vertical morphology of hypoxic zone. We contextualize the role of physical-biogeochemical drivers to the spatially variable hypoxia in a conceptual diagram. The findings would considerably contribute to our understanding of the spatially variable hypoxia in river plume-upwelling-front coupled estuarine systems, providing a sound basis for prediction and simulation under anticipated future conditions.
Investigation on the grain size effect on the copper shaped charge jet stretching behavior
Authors: Jiang Yao , Gu Rui Cong , Wang Jing Tao , Xiao Qiang-Qiang , Huang Zheng-Xiang
Published: October 2022

The grain size effect on the shaped charge jet (SCJ) stretching process was analytically formulated and experimentally verified by penetration tests. The present analytical model predicts an optimum grain size for the SCJ performance, deduced from the concurrent effect of grain size on flow stress, strain rate sensitivity, and surface roughness. Specifically, reducing the grain size will improve the initial surface roughness and decrease the initial perturbation amplitude, favoring the SCJ stretching. On the other hand, the strain rate sensitivity and flow stress for copper increase with the decrease of grain size, facilitating the perturbation growth and leading to a premature breakup. Thus, the present analytical model predicts that the optimum grain size of the SCJ is about 1–5 μm. The penetration test verified that the shaped charge liner with an average grain size of about 3.6 ± 2.5 μm exhibited the largest penetration depth. The consistent results from the analytical model and the penetration experiments certify the feasibility of the present analytical model on the SCJ performance.
Pharmacokinetic data in pregnancy: A review of available literature data and important considerations in collecting clinical data
Authors: Coppola Paola , Kerwash Essam , Nooney Janet , Omran Amro , Cole Susan
Published: October 2022

Pregnancy-related physiological changes can alter the absorption, distribution, metabolism and excretion of medicines which may affect the safety and efficacy of the medicines administered in pregnancy. Pharmacokinetic data can thus be instrumental in supporting dose adjustments required in this population. This review considers the availability of published pharmacokinetic data for over 200 medicines of interest for use in pregnancy in the UK, to identify whether sufficient data currently exists, in principle, for any medicine or group of medicines to support dose adjustments to maintain maternal health through pregnancy. Very limited data was found for many of the medicines of interest. Nevertheless, well documented, large changes of exposure for some drugs, where data is available, highlights the urgent need to collect more data of good quality to inform appropriate doses, when needed, in this population. In addition, clinical study methodology can have an impact on the usefulness of the data and key clinical design aspects are highlighted for consideration in future clinical study design.
Effectiveness of acupuncture combined with rehabilitation training vs. rehabilitation training alone for post-stroke shoulder pain: A systematic review and meta-analysis of randomized controlled trials
Authors: Zhan Jie , Wei Xiaojing , Tao Chenyang , Yan Xiaoting , Zhang Peiming , Chen Rouhao , Dong Yu , Chen Hongxia , Liu Jianhua , Lu Liming
Published: October 2022

Background

Post-stroke shoulder pain (PSSP) is characterized by shoulder pain on the hemiplegic side, which can limit physical activity in patients with stroke. Acupuncture combined with rehabilitation training (AR) has been widely used in PSSP, but the evidence of its effectiveness is still unclear.

Objective

The study aimed to evaluate the effect and safety of AR vs. rehabilitation training (RT) alone on PSSP.

Methods

We searched PubMed, the Cochrane Library, the Chinese Biological Medicine Database (CBM), the Chinese Scientific Journal Database (VIP), China National Knowledge Infrastructure (CNKI), and the WAN FANG database for relevant studies from their inception to February 2022. Only randomized controlled trials (RCTs) comparing the effect of AR with RT alone on PSSP were considered. The primary outcome was shoulder pain. Secondary outcomes included upper limb motor function, activities of daily living (ADL), shoulder range of motion (ROM), and adverse events (AEs). Subgroup analysis and sensitivity analysis were also conducted. Quality assessment was implemented based on Cochrane risk of bias (ROB) criteria, which consist of seven items. When more than four items in a study were judged as low ROB, the overall quality of this study was considered low risk.

Results

A total of 40 studies were included in the qualitative analysis, and 35 (87.5%) studies with 2,554 patients were included in the meta-analysis. Of the 40 studies, 14 (35.0%) were of moderate-to-high quality. The meta-analysis results showed that AR is better than RT alone in reducing shoulder pain (MD −1.32, 95% CI −1.58 to −1.07), improving upper limb motor function (MD 6.81, 95% CI 4.95–8.67), ADL (MD 11.17, 95% CI 9.44–12.91), and shoulder ROM (internal rotation: MD 10.48, 95% CI 8.14–12.83; backward extension: MD 7.82, 95% CI 6.00–9.64; anteflexion: MD 12.88, 95% CI 5.47–20.29; external rotation: MD 11.40, 95% CI 6.17–16.64; abduction: MD 16.96, 95% CI 8.61–25.31) without obvious AEs.

Conclusion

AR may be better than RT alone for the improvement of shoulder pain, upper limb motor function, ADL, and shoulder ROM, without obvious AEs in patients with PSSP. However, considering the clinical and statistical heterogeneity, our findings need to be interpreted with caution. More rigorous RCTs in this area should be conducted in the future.

Systematic review registration

[www.crd.york.ac.uk], identifier [CRD42022326763].
Therapeutic strategies for oral lichen planus: State of the art and new insights
Authors: Didona Dario , Caposiena Caro Raffaele Dante , Sequeira Santos Antonio Manuel , Solimani Farzan , Hertl Michael
Published: October 2022

Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa. Several clinical subtypes of OLP have been reported, including the reticular and erosive one. On the one hand, reticular OLP is usually asymptomatic and is characterized by white streaks surrounded by well-defined erythematous borders. On the other hand, erosive OLP shows ulcerations and erosions surrounded by erythematous mucosa. While reticular OLP is relatively easy to control, erosive OLP is extremely painful and refractory to therapies, limiting the quality of life of the patients. In addition, treating erosive OLP is extremely tricky, and a gold standard treatment has not yet been established. However, several therapeutic approaches have been reported as effective, including systemic corticosteroids, systemic retinoids, and anti-interleukin (IL)-17/anti-IL-23 drugs. Indeed, our group and other several authors reported the effectiveness of anti-IL17, anti-IL12/23, and anti-IL23 agents in refractory OLP, highlighting the urgency of clinical studies on the use of anti-IL agents in OLP patients. In this paper, we reviewed the English- and German-language literature about therapeutic strategies for treating OLP, focusing on new systemic therapies for erosive OLP.
Comparative transcriptome profiling reveals a network of differentially expressed genes in Asia II 7 and MEAM1 whitefly cryptic species in response to early infection of Cotton leaf curl Multan virus
Authors: Farooq Tahir , Lin Qi , She Xiaoman , Chen Ting , Tang Yafei , He Zifu
Published: October 2022

Cotton leaf curl Multan virus (CLCuMuV) is a whitefly-vectored begomovirus that poses ramping threat to several economically important crops worldwide. The differential transmission of CLCuMuV by its vector Bemisia tabaci mainly relies on the type of whitefly cryptic species. However, the molecular responses among different whitefly cryptic species in response to early CLCuMuV infection remain elusive. Here, we compared early-stage transcriptomic profiles of Asia II 7 and MEAM1 cryptic species infected by CLCuMuV. Results of Illumina sequencing revealed that after 6 and 12 h of CLCuMuV acquisition, 153 and 141 genes among viruliferous (VF) Asia II 7, while 445 and 347 genes among VF MEAM 1 whiteflies were differentially expressed compared with aviruliferous (AVF) whiteflies. The most abundant groups of differentially expressed genes (DEGs) among Asia II 7 and MEAM1 were associated with HTH-1 and zf-C2H2 classes of transcription factors (TFs), respectively. Notably, in contrast to Asia II 7, MEAM1 cryptic species displayed higher transcriptional variations with elevated immune-related responses following CLCuMuV infection. Among both cryptic species, we identified several highly responsive candidate DEGs associated with antiviral innate immunity (alpha glucosidase, LSM14-like protein B and phosphoenolpyruvate carboxykinase), lysosome (GPI-anchored protein 58) and autophagy/phagosome pathways (sequestosome-1, cathepsin F-like protease), spliceosome (heat shock protein 70), detoxification (cytochrome P450 4C1), cGMP-PKG signaling pathway (myosin heavy chain), carbohydrate metabolism (alpha-glucosidase), biological transport (mitochondrial phosphate carrier) and protein absorption and digestion (cuticle protein 8). Further validation of RNA-seq results showed that 23 of 28 selected genes exhibited concordant expression both in RT-qPCR and RNA-seq. Our findings provide vital mechanistic insights into begomovirus-whitefly interactions to understand the dynamics of differential begomovirus transmission by different whitefly cryptic species and reveal novel molecular targets for sustainable management of insect-transmitted plant viruses.
Diversity and abundance of antibiotic resistance genes and their relationship with nutrients and land use of the inflow rivers of Taihu Lake
Authors: Fernanda Prilli Arista , Liu Shuang , Yuan Tianma , Ramalingam Bharathi , Lu Jing , Sekar Raju
Published: October 2022

Taihu Lake is the third largest freshwater lake in China and an important source for drinking water, flood protection, aquaculture, agriculture, and other activities. This lake is connected to many principal and small rivers with inflow from west and outflow on the eastern side of the lake and these inflow rivers are believed to significantly contribute to the water pollution of the lake. This study was aimed at assessing the diversity and abundance of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), and their relationship with water quality parameters and land use patterns. Water samples were collected from 10 major inflow rivers and the source water protection area of the Taihu Lake in spring and summer 2019. High-throughput profiling was used to detect and quantify 384 ARGs and MGEs and in addition, 11 water quality parameters were analyzed. The results showed that the number of ARGs/MGEs detected in each inflow river ranged from 105 to 185 in spring and 107 to 180 in summer. The aminoglycoside resistance genes were the most dominant types ARGs detected followed by beta-lactam resistance, multidrug resistance, macrolide-lincosamide-streptogramin B (MLSB) resistance genes, which contributed to 65% of the ARGs. The water quality parameters showed significant correlation with absolute abundance of ARGs. Furthermore, significant correlation between ARGs and MGEs were also observed which demonstrates potential gene transfer among organisms through horizontal gene transfer via MGEs. ARGs showed strong positive correlation with cultivated and industrial lands whereas, negative correlation was observed with river, lake, forest, land for green buffer, and land for port and harbor. The overall results indicate that the inflow rivers of Taihu Lake are polluted by various sources including multiple nutrients and high abundance of ARGs, which needs attention for better management of the inflow rivers of this lake.
Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae
Authors: da Silva Giarlã Cunha , Rossi Ciro César , Rosa Jéssica Nogueira , Sanches Newton Moreno , Cardoso Daniela Lopes , Li Yanwen , Witney Adam A. , Gould Kate A. , Fontes Patrícia Pereira , Callaghan Anastasia J. , Bossé Janine Thérèse , Langford Paul Richard , Bazzolli Denise Mara Soares
Published: October 2022

The RNA chaperone Hfq promotes the association of small RNAs (sRNAs) with cognate mRNAs, controlling the expression of bacterial phenotype. Actinobacillus pleuropneumoniae hfq mutants strains are attenuated for virulence in pigs, impaired in the ability to form biofilms, and more susceptible to stress, but knowledge of the extent of sRNA involvement is limited. Here, using A. pleuropneumoniae strain MIDG2331 (serovar 8), 14 sRNAs were identified by co-immunoprecipitation with Hfq and the expression of eight, identified as trans-acting sRNAs, were confirmed by Northern blotting. We focused on one of these sRNAs, named Rna01, containing a putative promoter for RpoE (stress regulon) recognition. Knockout mutants of rna01 and a double knockout mutant of rna01 and hfq, both had decreased biofilm formation and hemolytic activity, attenuation for virulence in Galleria mellonella, altered stress susceptibility, and an altered outer membrane protein profile. Rna01 affected extracellular vesicle production, size and toxicity in G. mellonella. qRT-PCR analysis of rna01 and putative cognate mRNA targets indicated that Rna01 is associated with the extracytoplasmic stress response. This work increases our understanding of the multilayered and complex nature of the influence of Hfq-dependent sRNAs on the physiology and virulence of A. pleuropneumoniae.