bluuebunny/crossref_metadata_2025 huggingface dataset:
Penerapan Model Pembelajaran Kooperatif Tipe Student Teams Achievement Division (STAD) Berbantuan Lembar Kerja Siswa
Authors: Lamut Tekla , Hariyani Sri , Dinnullah Riski Nur Istiqomah
Published: July 2022

Model pembelajaran kooperatif tipe STAD lebih menekankan pada aktivitas dan interaksi antara siswa dalam berprestasi yang maksimal. Tujuan dilakukannya penelitian ini untuk meningkatkan hasil pembelajaran matematika siswa kelas VII SMP Negeri 4 Ndoso pada materi bilangan bulat dengan menerapkan model pembelajaran kooperatif tipe STAD berbantuan LKS. Jenis penelitian ini dapat berupa 31 siswa kelas VII SMP Negeri 4 Ndoso mengikuti penelitian tindakan kelas sebagai subjek penelitian. Prosedur pengumpulan data yang digunakan meliputi tes, observasi, dan dokumentasi. Tahapan analisis data meliputi reduksi data, penyajian data, dan penarikan kesimpulan. Berikut langkah-langkah model pembelajaran STAD dengan berbantuan lembar kerja siswa: (1) menyampaikan materi, (2) memberikan tes/kuis secara individu, (3) membentuk beberapa kelompok, (4) membimbing siswa dalam menyelesaikan LKS, (5) mempresentasikan hasil kerja kelompok dan (6) memberikan penghargaan. Hasil analisis didapatkan bahwa hasil belajar siswa mengalami peningkatan hasil tes pada siklus I dan siklus II. Skor rata‑rata siswa mengalami kenaikan dari sebesar 77,41 pada siklus I menjadi sebesar 81,93 pada siklus II. Hasil observasi guru pada siklus I memenuhi kriteria baik dengan skor 72,5%, sedangkan pada siklus II memenuhi kriteria sangat baik dengan skor  90%. Berdasarkan temuan tersebut, dapat dinyatakan bahwa penerapan pembelajaran  Student Teams Achievement Divisions (STAD) berbantuan LKS meningkatkan hasil belajar matematika siswa kelas VII SMP Negeri 4 Ndoso pada materi bilangan bulat.
Penerapan Model Pembelajaran Discovery Learning Untuk Meningkatkan Keterampilan Proses Sains Dan Prestasi Belajar IPA
Authors: Betan Lukas Hali , Pranata Kurriawan Budi , Jufriadi Akhmad
Published: July 2022

Tujuan penelitian  untuk  meningkatkan  keterampilan  proses sains dan prestasi belajar IPA siswa kelas VIII SMP PGRI 6 Malang melalui model pembelajaran Discovery Learning. Peneliti memakai metode  Penelitian  Tindakan  Kelas (PTK) dan dilaksanakan dalam dua siklus. Merujuk pada hasil analisis penelitian, keterlaksanaan/pembelajaran siklus/I adalah 75% dan memiliki kualifikasi baik sedangkan siklus II adalah 84,89% berkualifikasi baik. Dari persentase keterampilan proses sains siswa siklus I adalah 73% berkualifikasi cukup baik dan siklus II adalah 83% berkualifikasi baik.  Model pembelajaran Discovery Learning mampu mendongkrak  prestasi belajar IPA terlihat pada perbandingan rata-rata prestasi belajar pra siklus sebesar 66,89% dengan kualifikasi cukup baik, mengalami peningkatan disiklus I menjadi 70,81% yang berkualifikasi cukup baik, pada siklus II senilai 73,11%  yang berkualifikasi cukup baik. Pada ketuntasan belajar, prestasi belajar pra siklus adalah 48,65% memiliki kualifikasi kurang baik, siklus I adalah 62,16% berkualifikasi cukup baik sedangkan siklus II meningkat menjadi 81,08% yang berkualifikasi baik. Merujuk pada hasil penelitian ini, maka bisa disimpulkan bahwa penelitian Model pembelajaran Discovery Learninag mampuh meningkatkan keterampilan proses sains dan presrasi belajar IPA siswa SMP PGRI 6 Malang
Pengaruh Model Jigsaw terhadap Hasil Belajar Matematika Siswa SMPN Unggulan Ohoijang Maluku-Tenggara
Authors: Fatubun Wilhelmina , Hariyani Sri , Marsitin Retno
Published: July 2022

Hasil belajar merupakan komponen penting dalam suatu kemajuan pembelajaran dan langkah-langkah pembelajaran, salah satu penyebab turunnya hasil belajar siswa adalah karena guru masih sering menggunakan strategi berbicara sehingga siswa merasa lelah dan terasing dalam belajar. Salah satu cara untuk mengatasi hal tersebut adalah dengan menggunakan model pembelajaran yang bermanfaat, penelitian ini bertujuan untuk mengetahui pengaruh model pembelajaran kooperatif tipe jigsaw terhadap hasil belajar siswa. Penelitian ini dilakukan di SMP Negeri Unggulan Ohoijang Maluku-Tenggara. Populasi dalam penelitian ini adalah semua siswa kelas VII. Sampel yang dipilih menggunakan teknik sampling rumpun, kelas VII B sebagai kelas eksperimen dan kelas VII A sebagai kelas kontrol. Jenis penelitian yang digunakan yaitu eksperimen dengan desain eksperimen semu dan pola Non-Equivalent Control Group Design. Berdasarkan uji normalitas dan uji homogenitas menunjukan kedua sampel tersebut berdistribusi normal dan homogen. Analisis data menggunakan uji-t, karena sig 0,000 < 0,05 maka Ho ditolak dan Ha diterima. Jadi dapat disimpulkan bahwa ada pengaruh yang signifikan dalam penggunaan model pembelajaran kooperatif tipe jigsaw terhadap hasil belajar matematika siswa.Kata Kunci: Kooperatif, Jigsaw, Hasil belajar
Pengembangan Sistem Koreksi Jawaban Esai Otomatis Menggunakan Naive Bayes Dan Pengujian Menggunakan User Acceptance Test (UAT)
Authors: Mariyani Ulva Dwi , Setiyaningsih Wiji , Agustina Rini
Published: July 2022

SMK Muhammadiyah 2 Pagak merupakan sekolah yang selalu mengadakan ujian sebagai evaluasi belajar. Tugas guru saat ujian adalah membuat soal, kunci jawaban, serta melakukan penilaian. Banyaknya mapel dan siswa yang diajar menyebabkan guru mengalami kendala pengoreksian soal esai. Dibutuhkan admin untuk membantu mencetak jawaban, dan guru mengoreksi manual dengan waktu 7-10 menit (5-10 soal) untuk 1 siswa dalam 1 mapel. Proses tersebut kurang efektif serta sering terjadi kesalahan penilaian. Siswa juga harus menunggu penilaian dengan waktu lama, dan tidak dapat mengetahui jawaban yang benar serta skor setiap soal. Dari permasalahan tersebut dibuatlah sistem yang membantu koreksi ujian esai menggunakan sistem koreksi otomatis dengan Naïve Bayes dan model pengembangan Rapid Application Development (RAD), serta menghasilkan sistem yang membantu guru dalam pengoreksian esai agar lebih efektif, membantu admin agar tidak mencetak jawaban, dan mempermudah siswa melihat jawaban yang benar setelah ujian. Hasil pengukuran kelayakan yang dilakukan menggunakan User Acceptance Test (UAT) diperoleh persentase rata-rata pada admin 97.22%, pada guru 90.48%, dan pada siswa 86.21%. Hasil pengujian independent sample T-test menghasilkan sig > 0.05 dengan hasil 0.716 > 0.05, dapat dinyatakan tidak ada perbedaan signifikan antara penilaian manual dan otomatis. Hal ini menunjukkan sistem koreksi otomatis jawaban esai telah sesuai perancangan yang dibuat.
Pengembangan Lkpd Elektronik Menggunakan Aplikasi Live Worksheet Materi Bangun Ruang Sisi Datar Pada Siswa Kelas Viii Smpn 17 Malang
Authors: Bombang Viktor , Trija Fayeldi  , Yuniar I.P. Pranyata
Published: July 2022

Penelitian ini bertujuan untuk mengembangkan LKPD Elektronik materi Bangun Ruang Sisi Datar menggunakan aplikasi Live Worksheet pada siswa SMPN 17 Malang berdasarkan asas kevalidan, kepraktisan dan keefektifan. Subjek uji coba dalam penelitian pengembangan ini adalah siswa kelas VIII SMPN 17 Malang. Penentuan subjek tersebut dilakukan secara acak yang terdiri dari 6 siswa untuk uji coba lapangan skala kecil dan 20 siswa untuk uji coba lapangan skala besar. Teknik pengumpulan data dilakukan dengan wawancara, quisioner dan tes hasil belajar berupa tes tertulis. Data dianalisis secara deskriptif kuantitatif dan kualitatif. Hasil penelitian menunjukan bahwa aspek kevalidan produk E-LKPD yang dikembangkan diperoleh skor 3,35 dengan persentase 83,82% dari ahli materi, skor 3,27 dengan persentase 81,82% dari ahli pembelajaran dan skor 3,5 dengan persentase 87,5% dari ahli media, sehingga aspek kevalidan termasuk dalam kategori sangat baik dan tidak perlu direvisi. Pada aspek kepraktisan diperoleh skor 3,4 dengan  persentase 85,54% dari hasil angket respon peserta didik uji coba lapangan skala kecil dan skor 3,44 dengan persentase  86% hasil angket respon peserta didik uji coba lapangan skala besar, tingkat kepraktisan masuk dalam kategori tinggi. Sedangkan pada aspek keefektifan, diperoleh persentase ketuntasan nilai peserta didik sebesar 90%, mengindikasikan bahwa E-LKPD masuk dalam tingkat keefektifan sangat baik.
The Impact of Online Education System at Student's and Teacher's During Covid-19 Pandemic in Pakistan
Authors: Hassan Karim  , Manahil Hafeez
Published: July 2022

This study aims to describe the impact of the online education system on students and teachers during the covid-19 pandemic in Pakistan. The research method uses qualitative research, namely literature studies by examining the results of previous research related to the implementation of the existing education system in Pakistan. There are several research results to be studied, which provide a description of policies in the implementation of education during the covid-19 pandemic in Pakistan. Data analysis techniques use descriptive qualitative analysis, with reference to Miles & Hubberman's analysis, namely data reduction, data display, and conclusion drawing. The results showed that the online education system during the covid-19 pandemic in Pakistan, includes 1) the availability of gadgets, insufficient online learning materials, no access or slow internet speed, and the shedding of burdens, this negatively affects the study of students in terms of minimum participation or absence of students and attendance in online classes, 2) online learning cannot produce the desired results in underdeveloped countries such as Pakistan,  where most students are unable to access the internet due to technical as well as monetary issues, 3) challenges faced by school teachers in providing online lessons through Google Classroom, Zoom, and Microsoft Teams such as high-cost Internet packages, uncooperative learners, low attendance of learners, trust in teacher technology, limited availability of educational resources, lack of ICT knowledge, and poor network infrastructure.
La nieve en el Macizo de las Ubiñas (Montañas Cantábricas) y sus implicaciones geomorfológicas
Authors: Gallinar Cañedo David , Ruiz-Fernández Jesús , García-Hernández Cristina
Published: July 2022

Se han examinado las interrelaciones entre la nieve y ciertos procesos geomorfológicos en el Macizo de las Ubiñas. A través del trabajo de campo, el uso de termorregistradores y el análisis de datos termopluviométricos, se ha realizado un seguimiento de la superficie ocupada por los neveros desde 2012 hasta 2018, monitorizado específicamente los cambios en la cubierta nival entre los años 2015 y 2018 a 2380 metros s.n.m. Los principales resultados apuntan a una gran proliferación de morfologías nivokarsticas, solifluidales, o bien vinculadas a los neveros y a los aludes, resultantes todas ellas de dicha interacción. El periodo de isotermia del suelo se mantiene durante 6 a 8 meses a partir de 1800 metros s.n.m., existiendo una relación clara entre su duración y la ganancia de altitud (secundariamente, la orientación norte). El año 2018 acumuló la mayor duración de la cubierta nival (143 días), siendo asimismo el único año en el que se alcanzaron espesores de 120 centímetros. El espesor alcanzado, junto con las temperaturas estivales, ha sido el principal condicionante para el desarrollo y conservación de los neveros, habiendo sido 2017 el año en el que ocuparon menos extensión (0,2 hectáreas), mientras que 2013 fue aquel en el que se mantuvieron en mayor número y extensión (2,8 hectáreas).
Manifestaciones orales de la COVID-19 y el rol del receptor ACE2: ¿qué se sabe hasta el　momento?
Authors: Luna-Mazzola Ivo
Published: July 2022

La COVID-19 es una enfermedad actualmente pandémica que preocupa a profesionales de todas las áreas de las Ciencias de la Salud. La literatura actual indica que ciertos componentes del sistema estomatognático como lengua y periodonto expresan los receptores ACE2, responsables de la unión del SARS-CoV-2 a la célula humana. Esta expresión repercute en un marcado compromiso organular, convirtiendo a la cavidad oral no solo en una importante vía de ingreso viral, sino que también en una región con posibilidad de diversas manifestaciones como la xerostomía, halitosis, alteración quimiosensorial, coinfección oportunista y lesiones de mucosa. El reporte de estas manifestaciones en etapas tempranas de la enfermedad da indicios de un posible carácter predictivo, pudiendo ser de gran utilidad para la derivación diagnóstica. En tal sentido, es importante que el profesional odontólogo sepa reconocer esta sintomatología de presentación temprana, pudiendo detectar presuntivamente pacientes con COVID-19 en quienes aún no se haya consolidado un compromiso sistémico significativo. Esto permitiría derivar a interconsulta médica con fines diagnósticos definitivos y, de estar en lo correcto, prevenir tanto contagios como el avance de la enfermedad en el paciente. Fundados en esta importante labor, el presente artículo de revisión sintetiza la evidencia científica publicada en los últimos 3 años (periodo 2019-2021) acerca de las manifestaciones orales de la COVID-19.
Investigación formativa y producción científica del docente odontológico peruano
Authors: Luna-Mazzola Ivo , Estela-Zamora Rosmery Lorena , Mattos-Vela Manuel Antonio
Published: July 2022

La investigación formativa junto con la producción científica docente son formas muy tempranas de enseñar el proceso investigativo, que implica una cultura de investigación que se debe instaurar desde el pregrado, teniendo en cuenta el fin de esta, que es la difusión del conocimiento generado mediante la publicación científica, para lo que se debe buscar superar las principales trabas que la producción científica docente presenta. En las universidades peruanas que imparten la carrera de Odontología, la investigación formativa se ha visto impulsada para fortalecer la futura investigación en el ámbito de ciencias de la salud, siendo la producción científica docente de suma importancia para la contribución del avance de la ciencia e implicancia del alumnado con dicha causa. El objetivo del presente trabajo es reflexionar sobre algunos aspectos de la producción científica docente y la investigación formativa en las facultades de odontología peruanas.
Matrix Injectivity Achieved in Deepwater Gas-Injector Wells
Authors: Carpenter Chris
Published: July 2022

This article, written by JPT Technology Editor Chris Carpenter, contains highlights of paper SPE 208809, “Deepwater Gas-Injector Wells: Overcoming the Challenge of Achieving Matrix Injectivity,” by Cedric Manzoleloua, Chau Nguyen, SPE, and Albert Okhrimenko, SPE, Schlumberger, et al. The paper has not been peer reviewed.

After a successful exploration phase, an opportunity to develop the Guyana Suriname Basin came to fruition. The drilling and completion campaign scope included planned gas-injector wells that required a breaker application for matrix injection. A reversible nonaqueous reservoir drill-in fluid (RDIF) system using a calcium bromide brine as the internal phase and formulated at a 50/50 oil/water ratio (OWR) was selected. The complete paper discusses the results of extensive laboratory tests for the selection, customization of both the RDIF and the delayed breaker, and the field performance of the two fluids.

Introduction

All wells in the field were completed either as openhole gravel-pack or stand-alone screen configurations using inflow control devices (ICDs) or autonomous ICDs (AICDs). The gas-injector wells were completed as standalone completions with ICDs. The primary objective of the gas-injector wells was to put the wells on direct injection with no opportunity to flow back or fracture the formation.
Theoretical Study on Optoelectronic and Various Quantum Chemical Properties of Essential Amino Acids: A Comparative Study
Authors: Lakhera Shradha , Rana Meenakshi Rana , Devlal Kamal
Published: July 2022

In the present work, a theoretical study has been performed targeting essential amino acids (EAA) Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, and predicted their different physical and chemical properties by using computational techniques. Amino acids (AA), a fundamental structural unit of protein are amino and carboxyl-rich compounds having electrophilic and nucleophilic regions in it. The reactivity of AA were determined by computing molecular electrostatic potential (MEP) surfaces, counter plots, dipole moment, band gap, global reactivity parameters, and polarizability parameters. Spectral analysis (UV-Vis, Raman) helps in studying their electronic and vibrational properties. The polarizability and first order hyperpolarizability parameters were also computed to detect the nonlinear optical (NLO) behavior of AA. The comparison done with reference NLO materials Urea, Phenyl urea, and 3-nitroaniline showed that Phenylalanine have higher hyperpolarizability and can better to be used as a potent NLO material.
Do Plant Traits Help to Design Green Walls for Urban Air Pollution Control: A Short Review of Scientific Evidences and Knowledge Gaps
Authors: Hellebaut Anaïs , Boisson Sylvain , Mahy Grégory
Published: July 2022

Green walls (GW) and living wall systems (LWS) are often claimed to have a positive effect on urban pollution problems if their plants composition is optimal (design of the LWS). An in-depth survey of the knowledge on plants traits maximizing GW effects on air pollution shows that this statement is made too quickly: there are still some large knowledge gaps. Robust conclusions can only be drawn for particulate matter (PM): leaves containing trichomes/hairs are the most effective to capture PM. The rougher the leaf is, the more PM it catches, and small leaves collect more PM than large leaves. After having analyzed the plant composition of six LWS existing in Belgium, we noticed that this knowledge on efficient plant traits is not used to design them with the goal to maximize their PM capture. In addition, studies about other pollutants and other traits are required to optimize the GW plant composition and to maximize their effects on air quality.
Combined imputation, inverse propensity weight, and bootstrap approach to estimate treatment effects on in-hospital mortality: an ELSO registry analysis
Authors: Greco Kimberly F. , O’Halloran Conor P. , Alexander Peta MA. , Weller Edie
Published: July 2022

While multicenter patient registries are a valuable tool for understanding diseases and exposures that affect small patient populations, retrospective registry studies are particularly prone to imbalances in baseline covariates, confounding by indication, and exposure misclassification due to missing data. This paper describes an analysis performed on the Extracorporeal Life Support Organization (ELSO) registry to evaluate the comparative effectiveness of two treatments – centrifugal versus roller blood pumps – to reduce in-hospital mortality among pediatric extracorporeal membrane oxygenation (ECMO) patients. We outline a combined imputation, inverse propensity weight, and bootstrap approach to address potential sources of bias and obtain a valid estimate of treatment effectiveness.
Total pulpotomy for extensive decay in permanent molar: a clinical randomized controlled trial
Authors: AKDAĞ Uğur , DEMİR Pınar
Published: July 2022

Objectives: This clinical study aimed to compare the clinical and radiographic success of CEM and two other pulpotomy materials in total pulpotomy applied to permanent teeth. Materials and Method: In our study, 48 permanent molar teeth with symptomatic irreversible pulpitis of 40 pediatric patients aged 5-12 years were included. Teeth were randomly divided into 3 groups. Total pulpotomy was performed with Calcium Hydroxide (CH), Mineral Trioxide Aggregate (MTA), and Calcium Enriched Material (CEM). Patients were invited for control at 3, 6, 12, 15, and 18 months for clinical and radiographic examination. The clinical and radiographic successes of 3 different materials were compared at all follow-up periods. Results: At the 6th, 12th, and 15th months, the MTA and CEM groups were statistically more successful than the CH group clinically and radiographically (p<0.05). The clinical and radiographic success rates of the CH, MTA, and CEM groups at the 18th month were 60%, 64.7%, and 68.8%, respectively, and there was no statistically significant difference between the 3 groups (p>0.05). Conclusions: We found that CEM was as successful as CH and MTA in the treatment of total pulpotomy applied to permanent molars with symptomatic irreversible pulpitis. Clinical Relevance: CEM is a promising material for different treatment modalities in pediatric dentistry and endodontics, without the major disadvantages of MTA.
The Influence of Chile’s Food Labeling and Advertising Law and Other Factors on Dietary and Physical Activity Behavior of Elementary Students in a Peripheral Region: A Qualitative Study
Authors: Pfister Franziska , Pozas Claudia
Published: July 2022

Background: In 2016, Chile implemented the Food Labeling and Advertising Law to fight childhood obesity through front-of-package food labelling, marketing restrictions and school activities and programs. Nevertheless, little is known on its influence on key stakeholders in vulnerable peripheral regions of the country. This study aimed at identifying important influencing factors including the Food Labeling and Advertising Law on dietary habits and physical activity patterns of second graders in Chile, as perceived by school representatives and the children themselves.Methods: Semi-structured interviews with four school directors and 17 groups of three second graders, informal interviews with 9 key teachers and 4 food services staff complemented with documented observations were carried out in four primary schools of the Chilean city of Punta Arenas, in the Magallanes Punta Arenas region. The different sources allowed for triangulation of results.Results: FLAL seems to have a negligible influence on young children’s diet and physical activity in the study region. Barriers are children’s deeply rooted dietary habits, excessive screen-time, the parents’ lacking problem awareness, limited time for parenting practices and bad role modeling. Environmental barriers are overloaded schools due to insufficient coordination between governmental entities, lacking funds for sound teacher training and unsafe neighborhoods limiting children’s play.Conclusions: Policy interventions aimed at reducing childhood obesity need to include and empower schools, families and local communities as active partners and consider their framework conditions for greater influence on dietary habits and physical activity.
Bacterial community analysis of Rangori spring of Uttarakhand, India by 16S-rRNA Gene sequencing
Authors: CHHIMWAL MONIKA , Pandey Diksha , Srivast Rajeev kumar
Published: July 2022

The focus of this research is to conduct a large-scale metagenomic investigation of a notable spring in Uttarakhand i.e., Rangori spring in the ecologically unique region of Ramnagar in order to identify the resident microbial population. The sample was collected from Ramnagar, Nainital district of Uttarakhand the identification. The goal of this study is to look at the bacterial diversity in spring water. The MiSeq high-throughput sequencing was used to analyze and compare the bacterial diversity and community composition of samples from spring water samples. Overall, 519 optimized reads were obtained from the samples based on high-throughput sequencing of the V4 region of the 16S rRNA gene. Bacterial species detected in these samples covered 46 phyla, 53 orders, 121 families, 422 genera and 521 species. According to the analysis it was found that the samples included a plethora of bacterial variety. The dominating bacteria's distribution features revealed patterns of a high number of unusual species and a few common types. Taxonomic assignment analysis indicated that Proteobacteria, Bacteriodes, OD1, Cyanobacteria Planctomycetes, Actinobacteria, Acidobacteria, Chloroflexi, Chlamydiae and Verrucomicrobiadominated in the water and accounted for 93.36% at phylum level. The predominant groups were Heliscomenobacter, Lacibactor, Lysobacter, Novosphingobium, Planctomyces, Reyranella, Rheinheimera, Rhodobacter, Sediminibacterium, Sphingobiumat genus level. At species level vibrioides, somerae, colicanis, prausnitzii, rhizosphaerae, cauensis, brunescens, australicum ,massiliensis, yanoikuyaewere the abundant taxa, while vey small community of Archaea were presented: Methanocullaus, Cenarchaeu, Candidatus Nitrososphera This research deepens the understanding on microbial community in spring water and provide references for the association of bacterial composition and diversity along with their significance.
IMproving PULmonary hypertension Screening by Echocardiography: IMPULSE
Authors: Slegg Oliver Graham , Willis James Alexander , Wilkinson Fiona , Sparey Joseph , Wild Christopher Basil , Rossdale Jennifer , Ross Robert Mackenzie , Pauling John D , Carson Kevin , Kandan Sri Raveen , Oxborough David , Knight Daniel , Peacock Oliver , Suntharalingam Jay , Coghlan Gerry John , Augustine Daniel Xavier
Published: July 2022

Background: The World symposium on Pulmonary Hypertension (PH) has proposed that PH be defined as a mean Pulmonary Artery Pressure (mPAP) >20mmHg as assessed by Right Heart Catheterisation (RHC). Transthoracic Echocardiography (TTE) is an established screening tool used for suspected PH. International guidelines recommend a multi-parameter assessment of the TTE PH probability although effectiveness has not been established using real world data.Study aims: To determine accuracy of the European Society of Cardiology (ESC) and British Society of Echocardiography (BSE) TTE probability algorithm in detecting PH in patients attending a UK PH centre. To identify echocardiographic markers and revised algorithms to improve the detection of PH in those with low/intermediate BSE/ESC TTE PH probability.Method: TTE followed by RHC (within 4 months) was undertaken in patients for suspected but previously unconfirmed PH. BSE/ESC PH TTE probabilities were calculated alongside additional markers of right ventricular (RV) longitudinal and radial function, and RV diastolic function. A refined IMPULSE algorithm was devised and evaluated in patients with low and/or intermediate ESC / BSE TTE PH probability.Results: Of 310 patients assessed, 236 (76%) had RHC-confirmed PH (average mPAP 42.8±11.7). Sensitivity and specificity for detecting PH using the BSE/ESC recommendations was 89% and 68%, respectively. 36% of those with low BSE/ESC TTE probability had RHC-confirmed PH and BSE/ESC PH probability parameters did not differ amongst those with and without PH in the low probability group. Conversely, RV free wall longitudinal strain (RVFWLS) was lower in patients with vs. without PH in low BSE/ESC probability group (-20.6%±4.1 vs -23.8±3.9%) (P<0.02). Incorporating RVFWLS and TTE features of RV radial and diastolic function (RVFAC and IVRT) within the IMPULSE algorithm reduced false negatives in patients with low BSE/ESC PH probability by 29%. The IMPULSE algorithm had excellent specificity and positive predictive value in those with low (93% / 80%, respectively) or intermediate (82% / 86%, respectively) PH probability.Conclusion: Existing TTE PH probability guidelines lack sensitivity to detect patients with milder haemodynamic forms of PH. Combining additional TTE makers assessing RV radial, longitudinal and diastolic function enhance identification of milder forms of PH, particularly in those who have a low BSE/ ESC TTE PH probability.
The unique diagnostic and management challenge of a patient with concomitant Anti-Interferon-Gamma Autoantibody Associated Immunodeficiency Syndrome, IgG4-Related Disease, and treatment refractory disseminated Mycobacterium Avium Complex infection.
Authors: Boyle Spencer , Hagiya Ashley , Nguyen Minh-Vu H. , Liebman Howard , Lee Jin Sol G.
Published: July 2022

Background
Anti-interferon-gamma autoantibody-associated immunodeficiency syndrome is a rare and underrecognized adult onset immunodeficiency syndrome associated with severe opportunistic infections such as disseminated nontuberculous mycobacterium. Few cases have documented a relationship with IgG4-related disease. Concomitant diagnoses of these diseases present a diagnostic and management challenge.
Case Presentation:
A 61 year old man of Southeast Asian descent with pulmonary mycobacterium avium complex infection presented to our hospital system with a new skin rash and worsening lymphadenopathy. He was eventually diagnosed with IgG4-related disease through excisional nodal biopsy. He was managed with immunosuppressive treatment with prednisone, rituximab and cyclophosphamide. He later re-presented with disseminated mycobacterium avium complex infiltration of his joints, bones and prostate. Original titers of anti-interferon-gamma autoantibodies were falsely negative due to being on immunosuppressive therapy for his IgG4-related disease. However, anti-interferon-gamma autoantibody titers were re-sent after immunosuppression was held and returned strongly positive.
Conclusions
This case reviews diagnostic criteria and discusses management strategies with existing challenges in treating a patient with concomitant adult onset immunodeficiency syndrome, IgG4-related disease and a disseminated mycobacterial avium complex infection.
Effectiveness and cost-effectiveness of a telemedicine programme for preventing unplanned hospitalisations of older adults living in nursing homes: The GERONTACCESS Cluster Randomized Clinical Trial
Authors: Gayot Caroline , Laubarie-Mouret Cecile , Zarca Kevin , Mimouni Maroua , Cardinaud Noelle , Luce Sandrine , Tovena Isabelle , Durand-Zaleski Isabelle , Laroche Marie-Laure , Preux Pierre-Marie , Tchalla Achille
Published: July 2022

OBJECTIVE: The GERONTACCESS trial evaluated the utility and cost-effectiveness of a gerontological telemedicine (TLM) programme for preventing unplanned hospitalisation of residents living in nursing homes (NHs) in regions lacking medical facilities (“medical deserts”). DESIGN: GERONTACCESS was a 12-month, multicentre, prospective cluster-randomised trial conducted in NHs. The intervention group underwent TLM assessments every 3 months. The control group received the usual care. In both groups, comprehensive on-site assessments were conducted at baseline and the final visit. Care requirements were documented throughout the study.SETTING AND PARTICIPANTS: NH residents aged > 60 years with multiple chronic diseases.METHODS: The study outcomes were the proportion of patients who experienced avoidable and unplanned hospitalisation, and the incremental cost savings per quality-adjusted life years from baseline to the 12-month follow-up.RESULTS: Of the 426 randomised participants (mean ± standard deviation age, 87.2 ± 7.6 years; 311 [73.0%] women), 23.4% in the intervention group and 32.5% in the control group experienced unplanned hospitalisation (odds ratio [OR] = 0.73, 95% confidence interval [CI] 0.43 to 0.97; p = 0.034). Each avoided hospitalisation in the intervention group saved $US 3,846. CONCLUSIONS AND IMPLICATIONS: The results of GERONTACCESS revealed that our gerontological, preventative TLM program significantly reduced unplanned hospitalisations. This innovative intervention limited disease progression and promoted a healthy lifestyle among NH residents.TRIAL REGISTRATION Clinicaltrials.gov, NCT02816177, registered June 28, 2016.
Association between serum albumin and 60-day mortality in Chinese Hakka patients with non-APL acute myeloid leukemia: A Retrospective cohort study
Authors: Xiao Zuomiao , Li Haibo , Xiao Dejun , Liu Yulan , Chen Xianchun , Luo Shi , Ji Yanhong
Published: July 2022

Background
Acute myeloid leukemia (AML) is the main type of adult leukemia, and 60-day mortality is a vital clinical problem that doctors have to face at the begin with treatment. Studies on the association between serum albumin and 60-day mortality from AML (non-APL) are limited.
Methods
In this retrospective cohort study, ALB was measured after admission in all patients diagnosed with primary AML from the Affiliated Ganzhou Hospital of Nanchang University between January 2013 and May 2021. The outcome was all-cause, 60-day mortality. Multivariable Cox regression analyses were performed to calculate the adjusted hazard ratio (HR) and its corresponding 95% confidence interval (CI).
Results
This study included 394 primary AML patients. The overall 60-day mortality was 29.2% (115/394); it was 43.8% (57/130), 27.5% (36/131), and 16.5% (22/133) for ALB quantile1 (Q1, < 34.5 g/L), quantile 2 (Q2, 34.5–38.5g/L), and quantile 3 (Q3, ≥ 38.6g/L), respectively (P = 0.001). After adjusting for potential confounders, we found an association between a 9% decrease in 60-day mortality rate and a 1 g/L increase in ALB level (HR = 0.91, 95% CI: 0.86–0.96, P = 0.001), which was associated with 38% and 70% decreases in 60-day mortality rates in Q2 (HR = 0.62, 95% CI: 0.37–1.04, P = 0.072) and Q3 (HR = 0.30, 95% CI: 0.15–0.60, P = 0.001), respectively, compared with that in Q1. Similar results were obtained after subgrouping based on an ALB level of 35 g/L (HR = 0.57, 95% CI: 0.35–0.91, P = 0.020).
Conclusions
Serum albumin was significantly associated with 60-day mortality of primary AML, which has important clinical significance. Further investigation is warranted.
Management of type 1 hypersensitivity reactions to antituberculosis drug: Succesful Desensitization
Authors: KATRAN ZEYNEP YEGİN , Bulut İsmet , Babalık Aylin , Keren Metin
Published: July 2022

Objective: Type 1 hypersensitivity reaction includes reactions such as dry itching, rash, urticaria, angioedema, and anaphylaxis that usually occur within the first hour after taking the drug. In this study, it was aimed to investigate the prevalence of type 1 hypersensitivity reaction under treatment and treatment management in tuberculosis patients.Methods: The study is a case series; All of the patients who were hospitalized between 01.02.2015- 01.05.2021 were examined. All patients who developed a drug-induced type 1 hypersensitivity reaction were included. Antituberculosis drugs were given with the protocol made by Buhari et al. However, unlike what is stated in the protocol, pyrazinamide was given last during the administration of the drugs.Results: 2677 patients received inpatient tuberculosis treatment; type 1 hypersensitivity reactions were seen in 94 (3.5%) patients. Due to missing data in the file, 81 patients were included in the study. 44 (54.3%) of the cases were women; mean age (mean ± SD) 50.7 ± 17.69 years; 76 (93.8%) of them are citizens of the Republic of Turkey; 58 (71.6%) of them were diagnosed bacteriologically; 65 (80.2%) of them were pulmonary tuberculosis. The most common skin finding was urticaria in 49 (60.5%). The drug responsible for the most common reaction was pyrazinamide. In 49 (60.5%) cases, drugs were given by desensitization and it was successful. The duration of treatment was 7.91 ± 2.5 months (6-18 months). When evaluated in terms of treatment results, 68 (84%) patients successfully completed the treatment.Conclusion: Our study is the largest series of patients in which the demographic and clinical characteristics of type 1 IgE-mediated hypersensitivity developed under antituberculosis treatment were examined. An applicable, understandable and successful diagnostic process roadmap and desensitization scheme that can be used in this situation are presented.
The Effects of Joint Hypermobility on Pain and Functional Biomechanics in Adolescents with Chronic Musculoskeletal Pain
Authors: Black William , Wright Laura A. , DiCesare Christopher A. , Thomas Staci , Pfeiffer Megan , Kitchen Katie , Ting Tracy V. , Williams Sara E. , Myer Gregory D. , Kashikar-Zuck Susmita
Published: July 2022

Background: Joint hypermobility (JH) is a common clinical finding amongst hereditary connective tissue disorders that is observed in pediatric rheumatological settings, and often associated with chronic pain. JH may also contribute to deficits in physical functioning and physical activity, but previous findings have been inconsistent. It is possible that physical activity impairment in JH may be due to chronic aberrant movement patterns subsequent to increased joint laxity. Within a larger study of juvenile onset fibromyalgia (JFM), a secondary analysis was conducted to explore whether adolescents with JFM and JH differed from non-JH peers in terms of pain, daily functioning, and movement biomechanics during a moderately vigorous functional task. Findings: Thirteen adolescents (36.1%) from the larger sample of adolescents with JFM (N=36) met criteria for JH. Those with JH exhibited poorer overall functioning but there were no differences in pain. Those with JH exhibited decreased hip flexion and frontal plane hip moment (e.g., resistance to dynamic valgus) during the landing phase (early stance) and greater hip and knee transverse plane moments during the propulsion phase (late stance) of the drop vertical jump task (DVJ). No other differences in lower extremity biomechanics were observed between study groups. Conclusions: There were small but notable differences in biomechanics between patients with JFM who also had JH versus those without JH during a landing and jumping task (e.g., DVJ). These differences may indicate decreased joint stiffness during landing, associated with increased joint laxity and decreased joint stability, which may put them at greater risk for injury. Further study is warranted to examine whether these biomechanical differences in patients with JFM and JH affect their response to typical physical therapy or exercise recommendations.
PCSK9 Knockdown can improve myocardial ischemia/reperfusion injury by inhibiting autophagy
Authors: Huang Guangwei , Lu Xiyang , Duan Zonggang , Zhang Kai , Xiong Xinlin , Lin Mu zhi , Li Chao , Li Yunquan , Xu Lei , Zhou Haiyan , Luo Zhenhua , Li Wei
Published: July 2022

Background and Aim: This study aims to investigate the effect and mechanism of proprotein convertase subtilisin/Kexin type 9 (PCSK9) on myocardial ischemia-reperfusion injury (MIRI) and provide a reference for clinical prevention and treatment of acute myocardial infarction (AMI).METHODS: We established a rat myocardial ischemia/reperfusion (I/R) model and AC16 hypoxia/reoxygenation (H/R) model. A total of 48 adult male Sprague-Dawley rats were randomly assigned to three groups (n=16): control, I/R, and I/R +SiRNA. In I/R and I/R +siRNA groups, myocardial ischemia was induced via occlusion of the left anterior descending branch (LAD) of the coronary artery in rats in I/R group for 0.5 h and reperfused for three days. To assess the myocardial injury, the rats were subjected to an electrocardiogram (ECG), cardiac function tests, cardiac enzymes analysis, and 2,3,5-triphenyl tetrazolium chloride (TTC)/Evan Blue (EB) staining. Meanwhile, hematoxylin-eosin (HE) staining was used to detect morphological changes in cardiomyocytes in each group, and the level of myocardial fibrosis was quantified using Masson trichrome staining. Differences in the expression of autophagy-level proteins and Bcl-2/adenovirus E1B 19-kDa interacting protein (Bnip3) signaling-related proteins were determined by protein blotting.RESULTS: I/R and H/R injury increased the expression level of PCSK9, activated the downstream Bnip3, and subsequently triggered autophagy. In vitro and in vivo experimental studies revealed that siRNA knockdown of PCSK9 resulted in reduced expression of the autophagic protein Beclin-1, light chain 3 (LC3) compared to normal control-treated cells and control-operated groups. Simultaneously, the presentation of the autophagic pathway Bnip3 was downregulated. Furthermore, PCSK9-mediated small interfering RNA (siRNA) group injected into the left ventricular wall significantly improved cardiac function and myocardial infarct size, as well as the expression of mRNA of Recombinant Human Interleukin-1β（IL-1β. Moreover, Nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) was significantly downregulated and reduced the inflammatory response compared with the I/R group.CONCLUSIONS: In ischemic/hypoxic circumstances, PCSK9 expression was dramatically increased. PCSK9 knockdown alleviated MIRI via the Bnip3-mediated autophagic pathway and improved inflammatory response, myocardial infarct size, and cardiac function.
Anti-inflammatory properties of curcumin and silver (I) nanocomplexes in inflammatory bowel diseases: in vitro and in vivo examination
Authors: Piotrowska Marta , Krajewska Julia , Talar Marcin , Długosz Olga , Banach Marcin , Fichna Jakub
Published: July 2022

Purpose
Inflammatory bowel disease (IBD) is characterized by chronic and relapsing inflammation of the gastrointestinal tract. Current treatment strategies of IBD are insufficient, expensive and connected with high toxicity. In search for alternatives that could help in the management of IBD without causing serious side effects, natural compounds attracted much attention recently. Curcumin is a natural, inexpensive compound with anti-inflammatory properties derived from rhizomes of Curcuma longa L. Concurrently, silver can suppress microbial activity and promote wound healing. Taking into consideration properties of both compounds, we decided to combine, with the use of nanotechnology, silver and curcumin to synergistically enhance their activity.
Methods
We obtained two formulations: curcumin and silver (I) nanoparticles complex (curcumin + nAg) and curcumin nanoparticles and silver (I) nanoparticles complex (ncurcumin + nAg). We assessed the anti-inflammatory effect of formulations in vitro in the LPS-stimulated RAW264.7 macrophages and in vivo in the mouse model of dextran sulfate sodium (DSS)-induced colitis.
Results
In vitro, both formulations inhibited nitric oxide (NO) release and in vivo complexes alleviated colitis.
Conclusion
In conclusion, our research showed that combination of curcumin with nAg improves curcumin’s anti-inflammatory properties and that using nanocurcumin instead of curcumin further enhanced this effect. Curcumin and silver (I) nanocomplexes may become a new treatment option in inflammatory bowel diseases.
La Culebra stream, a hydrological relic in the Guadalajara Metropolitan Area (Mexico)
Authors: Carrillo-González Karen Aidé , Zavala-Aguirre José Luis
Published: July 2022

In urban environments the existence of streams with ecological functionality, with an assembly of producers, consumers and degraders, with abundance governed by environmental variations and by the flow of nutrients, maintaining an aerobic process is becoming less frequent. La Culebra stream originates in Los Colomos Urban Forest (LCUF) within the Guadalajara Metropolitan Area (GMA), which is preserved almost naturally with few human interventions in the studied section. The stream flows from springs that emerge within the Urban Forest. But its waters are discharged into an urban channel that carries drainage water and stormwater runoff. In addition, there are no records of the water quality of La Culebra stream. It is of great interest to know its characteristics to propose management alternatives or a better use. Through the use of physicochemical techniques and test organisms, the behavior of macronutrients in the water, the existence of inhibition factors and the presence of coliforms were investigated during six months representative of the seasonality of the year 2018. In the last month of the study, it was implemented the analysis of sediment quality with the use of a specific test organism for the soil-water interface. In general, the stream was found without an excessive load of nutrients and with a variability in its physicochemistry and biology depending on the seasonal cycle. The system maintains adequate levels of dissolved oxygen to support the respiration of organisms in the water column and in particular the upper layer of the sediment with the highest demand for this gas. It was found that the oscillation of the annual temperature in the stream presents a delay of one to two months in relation to the atmospheric temperature. The finding of the inverse behavior between temperature and pH facilitated the interpretation of the availability of nutrients that maintain a production, based on photosynthesis, proportional to their availability. Through the use of EDTA as a chelating agent of metal cations, the increase in the production of the plants was determined and in this way the presence of an inhibition factor was revealed. Although coliform levels were high in all samples, we conclude that their presence is not from human origin, but of ducks and small mammals that normally inhabit the urban forest. We recommend conducting microbiological studies of groundwater to confirm the natural origin of coliforms. Likewise, it is recommended that studies be implemented to characterize the nature of the inhibiting agent that we have detected. Finally, it is recommended that the administrative authority of the Urban Forest manage funds for the dissemination and preservation of La Culebra stream as a subject of high environmental and cultural value in the GMA.
The Suppressive role of Nanoencapsulated Chia oil against DMBA-induced breast cancer through oxidative stress repression and tumor genes expression modulation in rats
Authors: makawy Aida El , Mabrouk Dalia M , Mohammed Shaimaa E , Abdel-Aziem Sekena H , Kader Heba A M Abd EL , Sharaf Hafiza A , Youssef Dalia A , Ibrahim Faten M
Published: July 2022

Chia oil is high in omega-3 fatty acids, which have been linked to a lower risk of many diseases, including cancer. Oil encapsulation is a promising method for preserving oil content and improving solubility and stability. This study aims to prepare nano-encapsulated Chia oil and explore its suppressive role against chemically induced breast cancer in rats. The oil was extracted from commercial Chia seeds and their fatty acids were analyzed using Gas Chromatography-Mass Spectrometry (GC/MS). The Chia oil nanocapsules were prepared by using sodium alginate as a loading agent. The ability of oil nanocapsules to scavenge free radicals was determined using the DPPH radical scavenging assay. Breast cancer was induced via a single-dose subcutaneous injection of 80 mg/kg dimethylbenz(a)anthracene (DMBA). Breast cancer models were orally treated with Chia oil nanocapsules at doses of 100 & 200mg/kg for one month. The suppressive effect of Chia oil nanocapsules was investigated through estimation of intracellular reactive oxygen species (ROS) and protein carbonyl, gene expression of tumor suppressor genes (BRCA 1 & 2, TP53) evaluation, and histopathological analysis. The administration of Chia oil nanocapsules significantly reduced the increase in ROS and PC levels induced in DMBA groups. On the molecular level, the mRNA expression levels of BRCA1, BRCA2, and TP53 were modulated in tumor tissue of rats given Chia oil nanocapsules. According to the histopathological analysis, nanocapsules improved the tissue architecture of breast tumors. These findings demonstrate the ability of Chia oil nanocapsules to inhibit cancer cells in the rat breast.