bluuebunny/crossref_metadata_2025 huggingface dataset:
Algılanan Yönetici Mizahının Üretkenlik Karşıtı İş Davranışları Üzerindeki Etkisi
Authors: ÖZDEMİR Burak , İPEK Melih
Published: October 2021

Bu araştırmada, algılanan yönetici mizahının üretkenlik karşıtı iş davranışları (ÜKİD) üzerindeki etkisinin Genişletme ve İnşa Etme Teorisi (GİET) kapsamında incelenmesi amaçlanmaktadır. Bu amaca uygun olarak ağaç, ağaç ürünleri ve mantar ürünleri imalatı sektöründe faaliyet gösteren üç firmanın 228 çalışanından veriler kolayda örnekleme yöntemiyle elde edilmiştir. Araştırma bulguları alaycı mizahın bireylerarası sapma davranışı ve örgüte yönelik sapma davranışını pozitif yönde etkilediğini göstermektedir. Ayrıca reddedici mizahın bireylerarası sapma davranışını negatif yönde, üretici-sosyal mizahın ise örgüte yönelik sapma davranışını pozitif yönde etkilediği belirlenmiştir. Bu doğrultuda bulgular Genişletme ve İnşa Etme Teorisi çerçevesinde tartışılmış ve kültürel bağlamın sonuçlar üzerinde etkili olduğu sonucuna varılmıştır.
Uso de especies nativas como plantas ornamentales en el Distrito Metropolitano de Quito
Authors: Andrade Jazmina C. R. , Cruz-Cárdenas Jorge , Oleas Nora H.
Published: October 2021

INTRODUCCIÓN. El crecimiento de las ciudades ha afectado considerablemente los sistemas ecológicos, haciendo necesaria la creación de espacios verdes. La flora nativa es una buena opción para dichos espacios, debido al mínimo costo de mantenimiento, su adaptación a las temperaturas, precipitaciones y resistencia a plagas. OBJETIVO. Conocer la factibilidad del uso de plantas nativas como ornamentales en áreas urbanas del Distrito Metropolitano de Quito. Específicamente esperamos conocer la oferta y demanda de plantas ornamentales en el mercado. Y también queremos proponer especies nativas con potencial uso ornamental. MÉTODO. Se realizaron encuestas a comerciantes en Nayón acerca de las plantas que comercializan. Además, se realizaron consultas bibliográficas, entrevistas y visitas de campo para identificar las especies con potencial ornamental. RESULTADOS. Las plantas ornamentales exóticas son las que ocupan la mayor parte del mercado. Los comerciantes de plantas ornamentales por un lado no conocen cuales son las especies de plantas nativas, pero por el otro les otorgan características positivas. Presentamos una lista con 27 especies nativas con potencial ornamental. DISCUSIÓN Y CONCLUSIONES. Dadas sus características intrínsecas, así como el valor que se les dan a las plantas nativas, consideramos tienen potencial para ser utilizadas como ornamentales y formar parte del paisaje urbano.
Validation of Bengali Modified Barthel Index in aged patients of Bangladesh
Authors: Saha Sujan , Islam Md Tanvir , Ahsan Md Shamsul , Md Moniruzzaman -
Published: October 2021

Functional independence is an integral part for reflection of quality of living. Many scales have been utilised for it and the Barthel Index (BI) is one of them, which later modified on 10 activities having the score range of 0-20. English version of the Modified Barthel Index (MBI) is widely used to measure disability also in Bangladesh and an important tool for assessment of activities of daily living in aged patients. However, a validated and culturally adapted Bengali version of MBI has not been produced yet. This study describes the validation and reliability into Bengali of the modified 10-item version of MBI, and reports the procedures for testing its validity and reliability. For validation process a two phased study was conducted based on data from aged patients in out and in patient department of Bangabandhu Sheikh Mujib Medical University. After pre-testing of the Bengali version of MBI (B-MBI) among 30 aged patients in phase-1 of the study whereas total 129 patients were submitted to the adapted scale for testing its acceptability and internal consistency in second phase. The internal consistency by Cronbach’s alpha resulted equal to 0.880. The score was also tested against the Bengali adapted SF-36 (Short Form-36) to compare with physical functioning and mental health domains. It yielded Spearman’s correlation coefficient (rS) 0.787 (p<0.001)), indicating strong positive correlation of B-MBI with physical functioning part and a weak correlation (rs=0.191; p=0.031) was found with mental health domain of SF-36. Reliability was evaluated on 124 cases as 5 participants were missed for re-test; at test-retest was ICC=0.961 (95%IC: p<0.001). This study provides a new tool for professionals in Bangladesh to measure functional disability in aged patients both in inpatient and outpatient department of hospitals and also in the health and social care settings along the continuum of care.
Asian J. Med. Biol. Res. 2021, 7 (3), 238-248
Evaluation of an antimalarial herbal mixture and each extract for DNA and chromosomal mutations in Swiss albino mice and Allium cepa cells
Authors: Akeem Akinboro , Akinlabi Rufai Muhamed , Dorcas Ogunbameru Aanuoluwapo , Esther Oladosu Bolaji , Opeyemi Taiwo Modinat
Published: October 2021

Toxicological evaluation of herbal medicines is necessary because of possible adverse effects that may be associated with their consumption. This study screened antimalarial herbal recipe (containing leaves of Azadirachta indica and stem-bark of Alstonia boonei) and its individual plant’s extract for DNA and chromosomes mutation potentials following the DNA fragmentation and Allium cepa assays. Superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) activity of the recipe and each extract was determined. The kinds of phytochemicals present in them were determined using the FTIR technique. Water extracts of A. indica, and A.boonei at all the tested doses caused significantly lower DNA fragmentations than those of the controls. However, at 25.0% and 50.0% recipe, there was no significant difference in the percentage fragmented DNA compared to the positive control (0.05% sodium azide). Cell division was significantly inhibited by the extracts and recipe, chromosomal aberrations were not dose dependently induced and were significantly lowered than that caused by sodium azide (positive control). The individual extracts and their recipe significantly inhibited Root growth. However, 12.5% recipe promoted root growth that was not significantly different from that of distilled water (negative control). SOD and CAT activities of each of the extracts and their recipe were dose dependent and significantly higher than those of the controls. Water extract of A. indica significantly suppressed generation of malondialdehyde compared to water extract of A. boonei and recipe as well as the control. The individual extracts and their recipe contained phenolic phytochemicals. The obtained results show that extract of A. indica, A. boonei and their recipe have good antioxidant properties with strong mitodepressive and root growth inhibitory effects except at 12.5% recipe. However, A. indica extract seems to have least cyto-muta-genotoxic effects than water extract of A. boonei and the recipe in mice and A. cepa cells.
Asian J. Med. Biol. Res. 2021, 7 (3), 249-259
Sistem Monitoring Perawatan dan Perbaikan Fasilitas Gardu PT PLN Area Kota Metro
Authors: Wantoro Agus
Published: October 2021

PT  Perusahaan Listrik  Negara (PLN) PERSERO Area Metro  adalah  sebuah Badan Usaha Milik Negara (BUMN) yang beralamat di jalan Alamsyah Ratu Prawiranegara No. 99 15A Kauman Metro, Lampung. Penanggulangan laporan gangguan gardu distribusi, petugas sering kali kesulitan dalam pencarian lokasi gardu di lapangan, berimbas pada waktu kerja petugas, belum adanya sistem yang dapat melakukan monitoring terhadap perbaikan dan perawatan fasilitas, belum ada jadwal perawatan yang terkoordinir antara rayon satu dengan  rayon lainnya sering kali membuat petugas kewalahan, penggunaan peta konvensional yang sering membingungkan petugas baru dan lama, belum adanya sistem yang dapat menjembatani kantor rayon dengan gudang tekait permintaan barang. Salah satu solusi yaitu dengan dibuatnya sistem yang dapat memberikan informasi Pemetaan Perbaikan dan Perawatan Fasilitas PT. PLN Area Metro yang digunakan sebagai pengambilan informasi penyebaran distribusi maupun kerusakan area gardu listrik, menjembatani kantor rayon dan gudang dalam hal permintaan suku cadang, serta mengatur jadwal perawatan dan perbaikan gardu distribusi. Sistem yang dibangun telah diuji dengan ISO 25010 dengan  nilai persentase yang diperoleh menunjukkan kualitas perangat lunak dengan karakteristik functionality dan usability mempunyai skala sangat layak, lalu karakteristik reliability dengan skala layak, dapat disimpulkan bahwa fungsionalitas sistem dapat berfungsi dengan baik dan dapat digunakan
Rancang Bangun Geographic Information System (GIS) Sebagai Pengembangan Sistem Monitoring Area Perkebunan Berbasis IoT
Authors: Thereza Nadia , Saputra Iwan Pahendra Anto , Husin Zaenal
Published: October 2021

Operasional sektor perkebunan di Indonesia sebagian besar masih mengandalkan sistem konvensional yang menggunakan tenaga manusia untuk melakukan kontrol ke lapangan. Dengan kondisi tersebut, masih sulit jika ingin melakukan peningkatan kinerja operasional menjadi lebih efisien, efektif, dan produktif. Ditambah lagi, kondisi pandemi yang tengah dihadapi saat ini secara tidak langsung sangat berdampak dan berpotensi menurunkan angka produktivitas. Sistem operasional ataupun pengelolaan lahan perkebunan harus mengalami perubahan. Pemanfaatan teknologi dan inovasi sangat dibutuhkan untuk membantu mempertahankan ataupun meningkatkan kualitas dan kuantitas hasil produksi. Oleh sebab itu, dibutuhkan suatu rancangan sistem informasi berbasis website, yang bekerja menyampaikan informasi kondisi geografis suatu area secara real-time sebagai solusi dari permasalahan pemantauan kondisi geografis. Sistem informasi tersebut bekerja menggunakan informasi berupa data spasial (bereferensi geografis) yang dikenal dengan Sistem Informasi Geografis atau Geographic Information System (GIS).Tujuan penelitian ini adalah untuk  membangun GIS berbasis web guna memberikan informasi dan menggambarkan kondisi (normal, rawan, kritis) pada suatu area/lahan secara real-time. Metode penelitian ini terdiri dari lima tahapan, yaitu persiapan penelitian (studi literatur), pengkajian objek (observasi, analisis kebutuhan sistem), perancangan dan pembangunan GIS dan integrasi dengan IoT, analisis dan pengujian penerapan GIS, serta penarikan kesimpulan. Sistem informasi geografis yang dibuat adalah dengan menampilkan peta (maps) area yang dipantau. Perangkat lunak yang digunakan untuk menampilkan maps adalah Google Maps Platform yang mana pada platform tersebut terdapat interface yang disebut API (Application Programming Interface). Google Maps API digunakan untuk menghubungkan sistem informasi geografis yang berbasis website dengan google maps. GIS memberikan informasi dan menggambarkan kondisi geografis suatu area secara real-time, kondisi area yang normal ditandai dengan warna hijau, kondisi antara (rawan kritis) ditandai dengan warna kuning serta kondisi area yang kritis ditandai dengan warna merah. Dengan adanya sistem informasi geografis, maka membantu para pekerja lapangan dalam memantau kondisi geografis suatu area secara real-time tanpa harus berada langsung di lokasi. Berdasarkan hasil penilaian pengguna, sebagian besar menyatakan “sangat setuju” bahwa GIS ini bermanfaat, bersifat user-friendly, mudah diakses kapanpun dan di manapun, serta memiliki kecepatan akses yang baik. Selain itu, sebagian besar juga menyatakan “setuju” bahwa GIS mampu menampilkan informasi yang akurat dan dapat membantu pekerjaan.
Sistem Pakar Deteksi Awal Covid-19 Menggunakan Metode Certainty Factor
Authors: Fahindra Aulia Rahman , Al Amin Imam Husni
Published: October 2021

Virus corona 2019 (corona virus disease/COVID-19) merupakan virus baru yang muncul di Wuhan,China pada akhir tahun 2019, gejala umum yang ditimbulkan dari covid-19 adalah suhu tubuh naik, demam, mati rasa, batuk, nyeri di tenggorokan, kepala pusing, susah bernafas jika virus corona sudah sampai paru-paru. Untuk mengetahui seseorang terjangkit covid-19 pun cukup sulit karena memiliki gejala yang mirip dengan beberapa penyakit lainnya. Deteksi Covid-19 merupakan tahapan penting untuk mengenali secara dini pasien terduga Covid-19 sehingga dapat dilakukan langkah lanjutan. Salah satu cara pendeteksian adalah dengan sistem pendeteksi awal covid-19.  Dengan keadaan tersebut tentunya dibutuhkan sistem pakar yang dapat melakukan sebuah deteksi awal terhadap gejala covid-19 . Namun demikian dibutuhkan sebuah metode untuk mengakomodasi faktor-faktor yang ada, sehingga di dapat hasil yang akurat. Metode Certainty Factor dipilih untuk mengakomodasi faktor ketidakpastian untuk diubah menjadi faktor kepastian, certainty factor juga dapat menyatakan kepercayaan dalam sebuah kejadian (fakta atau hipotesa) berdasar bukti atau laporan pakar dengan menggunakan suatu nilai untuk mengasumsikan derajat keyakinan seorang pakar terhadap suatu data.Hasil Penelitian dapat membantu masyarakat untuk melakukan diagnosa mandiri, dan paramedis untuk melakukan diagnosa awal kepada pasien, sehingga dapat mempermudah semua pihak dalam menangani Covid-19.
Sistem Informasi Monitoring dan Reporting Quality Control Proses Laminating Berbasis Web Framework Laravel
Authors: Herdiansah Arief , Borman Rohmat Indra , Maylinda Sonia
Published: October 2021

PT Victory Chingluh Indonesia merupakan perusahaan yang bergerak dibidang industri manufacture yang memproduksi sepatu olahraga merk global, karena itu hasil proses produksi yang berjalan di setiap proses produksi harus merupakan hasil yang sesuai dengan standard kualitas yang sudah ditetapkan Perusahaan. Penelitian ini merupakan penelitian terapan yang dilakukan di bagian Quality Control (QC) PT Victory Chingluh Indonesia khususnya monitoring dan reporting proses laminating. Saat ini proses cek kualitas hasil produksi dan proses pembuatan laporan  QC belum dilakukan menggunakan sebuah sistem informasi berbasis komputer, sehingga peneliti merasa perlu untuk membantu mengembangkan sistem informasi monitoring dan reporting quality control. Dalam proses pengumpulan data, peneliti melakukan metode survey dan wawancana kepada staf terkait dibagian QC proses laminating. Peneliti menggunakan UML (Unified Modeling Language) dalam proses perancangan sistem informasi dan peneliti menggunakan bahasa pemrograman PHP dengan framework laravel dalam tahap pengembangan sistem. berbasis database dan web. Pada tahapan akhir pengembangan, sistem yang dihasilkan diuji dengan menggunakan metode pengujian black box. Sistem yang dihasilkan telah dapat membantu staf QC me-monitoring hasil pekerjaan QC dan membuat laporan QC hasil proses produksi dengan cepat dan tepat, sistem yang dihasilkan juga menghasilkan sebuah laporan dalam bentuk dashboard (grafik) yang dapat mempermudah manajemen untuk melakukan analisa laporan hasil produksi QC di PT Victory Chingluh Indonesia
Implementasi Metode SAW Dan TOPSIS Dalam Pemilihan Rumah Hunian Di Wilayah Semarang Barat
Authors: Al amin Imam Husni
Published: October 2021

Dalam berumah tangga memiliki tempat tinggal merupakan suatu kebutuhan yang wajib terpenuhi. Disayangkan banyak masyarakat yang kesulitan untuk menentukan rumah hunian yang sesuai dengan kebutuhan dan keinginan saat akan membeli tempat tinggal berupa perumahan. Hal tersebut karena minimnya pengetahuan masyarakat tentang seluk beluk perumahan yang tidak sebanding dengan banyaknya pilihan rumah yang ditawarkan. Sehingga dari permasalahan tersebut dibutuhkan suatu sistem pendukung keputusan untuk membantu masyarakat dalam menentukan rumah hunian yang akan dibeli dan tinggali. Sistem yang dibuat dapat membantu masyarakat untuk menentukan pilihan rumah hunian berdasarkan kriteria yang sudah disediakan oleh sistem dan ditentukan oleh pengguna. Kriteria yang digunakan meliputi type rumah dengan luas tanah, harga, material bangunan, fasilitas rumah dan akses jalan. Perancangan sistem pendukung keputusan ini mengadopsi dua metode yaitu hybrid SAW (Simple Additive Weighting) dan TOPSIS (Technique for Order Performance of Similarity to Idea Solution). Metode SAW digunakan untuk tahap pembobotan sedangkan metode TOPSIS digunakan untuk proses perangkingan. Masyarakat dapat menggunakan sistem ini dengan mudah karena berbasis website dimana pembuatannya menggunakan bahasa pemrograman PHP dan database MySQL
Instilling Character Values Using Jigsaw in Teaching Grammar
Authors: Baidawi Achmad , Rabi'ah Rabi'ah , Wafi Abdul
Published: October 2021

Building nation character should be always done specially by the teacher in the school bacause nation character is one of fundamental elements in preventing this nation from the attack of foreign or western cultures which are not suitable with our cultures. This study aimed at decribing how the character values inserted in teaching grammar using jigsaw and what values were inserted. The study used descriptive qualitative field research. The data obtained through observation, and interview. After analyzing the data, it was found that character values were inserted through jigsaw activity. The character values found were tolerance, democratic, curiousity, communicative, responsible. This reseach is expected to contribute to build nation character through teaching and learning process.
Способи захисту прав сторін корпоративного договору: проблемні питання теорії та практики
Authors: ДОРОШЕНКО ЛІНА
Published: October 2021

У статті проаналізовано теоретичні і практичні питання, що виникають при захисті прав сторін корпоративного договору.
Мета статті полягає у визначенні способів захисту прав сторін корпоративного договору та розробці пропозицій з удосконалення їх застосування.
Доведено, що існування двох понять – “корпоративний договір” і “договір між акціонерами товариства”, однакових за змістовним наповненням, але поіменованих по-різному, має штучний характер, та запропоновано уніфікацію понятійно-категоріального апарату, який позначає цей договір.
Досліджено досвід інших країн (Франції, Бельгії, Греції, Німеччини, США, Великої Британії) з питань встановлення в законодавстві та на практиці способів захисту прав сторін корпоративного договору. Встановлено, що в зарубіжних країнах сторони корпоративного договору використовують такі способи захисту прав, як відшкодування збитків, примусове виконання зобов’язання в натурі, інші превентивні механізми (судова заборона, встановлення обмежень щодо внесення змін до локальних актів корпорації), виключення учасника товариства за рішенням суду тощо. Визначено, що в українському законодавстві знайшли відображення лише такі способи захисту прав, як відшкодування збитків і компенсація.
Виокремлено способи захисту прав, які передбачені національним законодавством. Досліджено, які з них застосовуються на практиці сторонами корпоративного договору. Проаналізовано застосування таких способів захисту, як відшкодування збитків; визнання договору щодо прав на акції та/або прав за акціями, укладеного стороною договору між акціонерами на порушення корпоративного договору, недійсним; визнання правочину недійсним або визнання недійсними окремих його частин, компенсація. Визначено їх позитивні та негативні аспекти, окреслено перспективи їх застосування на практиці.
Проаналізовано судову практику щодо способів захисту прав сторін. Встановлено, що суд визнав обов’язковість корпоративного договору та можливість сторін застосувати на практиці положення, які передбачені договором. Доведено, що корпоративний договір стає інструментом, який здатний забезпечити бізнес-партнерам сталі правила поведінки, які можна захистити у суді.
На підставі проведеного дослідження зроблено висновок про доцільність закріплення в законодавстві переліку найбільш універсальних та ефективних способів захисту прав сторін корпоративного договору, якими можуть бути: відшкодування збитків, стягнення неустойки (штрафу, пені), виплата компенсації, визнання корпоративного договору або окремих його частин недійсними; визнання недійсними правочинів, укладених стороною корпоративного договору в порушення умов такого договору, тощо.
The Role of GAD65 Autoantibodies in the development of Type-1 Diabetes
Authors: Prasetyo Sri R.
Published: October 2021

AbstractThe finding of the autoantibodies to islet cells (ICAs) in type-1 diabetes patients is important for developing the fine tuning of individualized therapy. Antibody to Glutamate decarboxylase 2 (GAD65Ab) is the most reliable sign, since it has the most stable sensitivity as diagnostic tool for detecting type-1diabetes. As a key enzyme in gamma-Aminobutyric acid (GABA) synthesis, GAD65 damage caused by GAD65 antibodies (GAD65Abs) would lead to decrease in the amount of GABA vesicles released by b-cells. Decrease of GAD65 induced by GAD65Ab may endanger the paracrine or autocrine function of GABA, that mediated by γ-aminobutyric acid type A receptors (GABAAR) would depolarized the b-cells. The depolarization then increases intracellular Calsium (Ca2+) concentration that is needed for insulin release. The effect of GABA on b-cells is also important for proliferation and anti-apoptosis of b-cells. Moreover, decrease in GABA release also impairs the inhibiting effect of GABA on T-cell proliferation and inflammatory cytokines release that may end up with escalation of GAD65 damage.Keywords: Type-1 diabetes, autoantibody, GAD65Peran Autoantibodi GAD65 dalam Perkembangan Diabetes Tipe-1AbstractPenemuan autoantibodi terhadap sel pulau atau islet cells (ICA) pada pasien diabetes tipe-1 penting untuk mengembangkan penyesuaian terapi individual. Antibodi terhadap Glutamat dekarboksilase 2 (GAD65Ab) adalah tanda yang paling dapat diandalkan, karena memiliki sensitivitas yang paling stabil sebagai alat diagnostik untuk mendeteksi diabetes tipe-1. Sebagai enzim kunci dalam sintesis asam gamma-aminobutirat (GABA), kerusakan GAD65 yang disebabkan oleh antibodi GAD65 (GAD65Abs) akan menyebabkan penurunan jumlah vesikel GABA yang dilepaskan oleh sel. Penurunan GAD65 yang diinduksi oleh GAD65Ab dapat membahayakan fungsi parakrin atau autokrin GABA, yang dimediasi oleh reseptor asam γ-aminobutirat tipe A (GABAAR) akan mendepolarisasi sel. Depolarisasi kemudian meningkatkan konsentrasi kalsium (Ca2+) intraseluler yang diperlukan untuk pelepasan insulin. Efek GABA pada sel beta juga penting untuk proliferasi dan anti-apoptosis sel beta. Selain itu, penurunan pelepasan GABA juga merusak efek penghambatan GABA pada proliferasi sel T dan pelepasan sitokin inflamasi yang mungkin berakhir dengan peningkatan kerusakan GAD65.Kata kunci: Diabetes tipe-1, autoantibodi, GAD65
46, XY DSD (Disorder of Sex Development) : Diagnosis dan Tatalaksananya.
Authors: Felicia Mildi , Suhartono Beto
Published: October 2021

Disorder of Sex Development (DSD) is a congenital disorder that occurs in the development of chromosomes, gonads, and internal or external genital organ. DSD of 46 XY is a condition where the children with XY genotype is not able to have complete virilization of external genital. Determination phase is an initial phase of reproductive system development. Disruption of this phase may potentially lead to DSD.  Optimal care for children with Disorder of Sex Development requires a multidisciplinary team starting since neonatal period. Family and pregnancy history, complete physical examination and assessment of genital organ are the first step of ensuring the diagnose. Management of children with DSD are focusing on gender determination, hormone support therapy and surgical management. On the other hand, Children with XY genotype should be raised as a boy however if there is no responsive evidence in administration of androgen the children should be raised as a girl. Subsequent to prescribe the gender of the children, surgical management is a required treatment for removing unnecessary genital afterward.

Keywords: Disorders of Sex Development, DSD, 46 XY DSD
Gambaran Kepatuhan Mahasiswa Pre Klinik FK UKI dalam Menjalankan Protokol Kesehatan New Normal Pandemi Covid-19
Authors: Simanungkalit Bona
Published: October 2021

Abstrak

Kepatuhan masyarakat menjalankan protokol kesehatan berpengaruh menekan penularan Covid-19. Perilaku physical distancing mahasiswa Universitas Islam Negeri (UIN) Syarif Hidayatullah Jakarta hanya 55,9%.1 Perilaku hand wash mahasiswa Universitas Muhammadiyah Magelang 52,6%.2 Untuk mengetahui kepatuhan mahasiswa dalam melakukan tes kesehatan mandiri, pemeriksaan suhu, penggunaan masker, faceshield, hand hygiene, menjaga jarak, waktu berada di kampus, konsumsi vitamin C, dan membersihkan diri setelah pulang. Desain penelitian adalah deskriptif. Pengambilan data menggunakan kuesioner, pengambilan sampel dengan teknik cluster random sampling, jumlah sampel 269 mahasiswa. Kepatuhan mahasiswa dalam menjalankan tes kesehatan mandiri 56,3%; pemeriksaan suhu 94%; penggunaan masker 98,9%; faceshield 46,1%; hand hygiene 80,7%; physical distancing 52,4%; berada di Fakultas Kedokteran Universitas Kristen Indonesia dalam waktu singkat 95,1%; konsumsi vitamin C 28,6%; membersihkan diri 73,6%. Sebanyak 93,5% mahasiswa tidak patuh dalam menjalankan protokol kesehatan.

Kata Kunci: Kepatuhan, Mahasiswa FK UKI, Protokol Kesehatan, Covid-19
The Analysis of Fair Tales Complied by Yücel Feyzioglu with Respect to Desired Behaviours in Children
Authors: TOPRAK Mesut , KIBRIS İbrahim
Published: October 2021

The aim of the present research was to identify to what extent fairy tales incorporate the desired behaviors which are crucial for children’s development in various fields. For this purpose, 11 fairy tale books, written by Yücel Feyzioğlu, were examined, namely Dimitraş ile Pıtıraş, Aldar Köse, Alp ile Asamat Köprüsü, Yartı Kulak, Keloğlan ile Kahkaha Hanım, Cırttan ile Çilbik, Murkumama ile Çomotay, Pürüş Batur ile Aksıla, Er Tapıldı, Serçe Koyun Avlarkene, and İristu ile Akkağan. Since present study addresses the importance of fairy tales in children’s education of values, the population of the study is fairy tales books, and the research method employed is content analysis. The ethical, psychological, sociological, economy-related and other messages in the fairy tales in terms of desired behaviors were analysed. The findings revealed that the fairy tales penned by Yücel Feyzioğlu were of appropriate educational value, particularly with respect to psychological and ethical massages.
Disproof of the Riemann Hypothesis
Authors: Vega Frank
Published: October 2021

We define the function $\upsilon(x)=\frac{3 \times \log x+5}{8 \times \pi \times \sqrt{x}+1.2 \times \log x+2}+\frac{\log x}{\log (x + C \times \sqrt{x} \times \log \log \log x)} - 1$ for some positive constant $C$ independent of $x$. We prove that the Riemann hypothesis is false when there exists some number $y \geq 13.1$ such that for all $x \geq y$ the inequality $\upsilon(x) \leq 0$ is always satisfied. We know that the function $\upsilon(x)$ is monotonically decreasing for all sufficiently large numbers $x \geq 13.1$. Hence, it is enough to find a value of $y \geq 13.1$ such that $\upsilon(y) \leq 0$ since for all $x \geq y$ we would have that $\upsilon(x) \leq \upsilon(y) \leq 0$. Using the tool $\textit{gp}$ from the project PARI/GP, we note that $\upsilon(100!) \approx \textit{-2.938735877055718770 E-39} < 0$ for all $C \geq \frac{1}{1000000!}$. In this way, we claim that the Riemann hypothesis could be false.
An Efficient GUI-Based Clustering Software for Simulation and Bayesian Cluster Analysis of Single-Molecule Localization Microscopy Data
Authors: Kutz Saskia , Zehrer Ando C. , Svetlitckii Roman , Gülcüler Balta Gülce S. , Galli Lucrezia , Kleber Susanne , Rentsch Jakob , Martin-Villalba Ana , Ewers Helge
Published: October 2021

Ligand binding of membrane proteins triggers many important cellular signaling events by the lateral aggregation of ligand-bound and other membrane proteins in the plane of the plasma membrane. This local clustering can lead to the co-enrichment of molecules that create an intracellular signal or bring sufficient amounts of activity together to shift an existing equilibrium towards the execution of a signaling event. In this way, clustering can serve as a cellular switch. The underlying uneven distribution and local enrichment of the signaling cluster’s constituting membrane proteins can be used as a functional readout. This information is obtained by combining single-molecule fluorescence microscopy with cluster algorithms that can reliably and reproducibly distinguish clusters from fluctuations in the background noise to generate quantitative data on this complex process. Cluster analysis of single-molecule fluorescence microscopy data has emerged as a proliferative field, and several algorithms and software solutions have been put forward. However, in most cases, such cluster algorithms require multiple analysis parameters to be defined by the user, which may lead to biased results. Furthermore, most cluster algorithms neglect the individual localization precision connected to every localized molecule, leading to imprecise results. Bayesian cluster analysis has been put forward to overcome these problems, but so far, it has entailed high computational cost, increasing runtime drastically. Finally, most software is challenging to use as they require advanced technical knowledge to operate. Here we combined three advanced cluster algorithms with the Bayesian approach and parallelization in a user-friendly GUI and achieved up to an order of magnitude faster processing than for previous approaches. Our work will simplify access to a well-controlled analysis of clustering data generated by SMLM and significantly accelerate data processing. The inclusion of a simulation mode aids in the design of well-controlled experimental assays.
RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5
Authors: Xu Fenghua , Xiao Zhifeng , Fan Liqin , Ruan Guangcong , Cheng Yi , Tian Yuting , Chen Minjia , Chen Dongfeng , Wei Yanling
Published: October 2021

Objectives: Colorectal cancer (CRC) is one of the most common human malignancies. It was reported that the alterations in the DNA damage response (DDR) pathways are emerging as novel targets for treatment across different cancer types including CRC. RFWD3 plays a critical role in replication protein A (RPA)-mediated DNA damage in cancer cells. More importantly, RFWD3 can response to DNA damage by positively regulating p53 stability when the G1 cell cycle checkpoint is activated. However, the functional significance of RFWD3 in CRC has not been reported in the existing documents.

Materials and Methods: Here, we revealed high expression of RFWD3 in CRC tissues by IHC analysis and The Cancer Genome Atlas (TCGA) database. Besides, overexpression of RFWD3 in CRC cell lines was also confirmed by qRT-PCR and western blot assay. The Celigo cell counting method and wound-healing/transwell migration assay were applied to evaluate CRC cell proliferation and migration. The tumor growth indicators were quantified in nude mice xenografted with shRFWD3 and shCtrl RKO cells.

Results: The results indicated that RFWD3 knockdown restricted CRC development in vitro and in vivo. In exploring the downstream mechanism of RFWD3’s action, we found that RFWD3 could transcriptionally activate BIRC5 by interacting with E2F transcription factor 1 (E2F1). Accordingly, we identified BIRC5 as a downstream gene of RFWD3 regulating CRC. Subsequent loss- and gain- of function experiments demonstrated that upon overexpressing BIRC5 in RKO cells with down-regulated RFWD3, the inhibitory effects of cell proliferation, migration and colony formation could be reversed, while the capacity of cell apoptosis was ameliorated, suggesting that the effects of RFWD3 depletion was mainly due to BIRC5 suppression.

Conclusion: Taken together, this study revealed that RFWD3 participates in the occurrence and development of colorectal cancer via E2F1 transcriptional regulation of BIRC5.
Identification of a Hypoxia-Related Gene Signature for Predicting Systemic Metastasis in Prostate Cancer
Authors: Xia Haoran , Wang Jianlong , Guo Xiaoxiao , Lv Zhengtong , Liu Jingchao , Yan Qiuxia , Liu Ming , Wang Jianye
Published: October 2021

Background: Systemic metastasis is the main cause of death in patients with prostate cancer. It is necessary to establish a more accurate model to distinguish and predict patients with a high risk of metastasis to optimize individualized treatment.

Methods: In this study, it was determined that hypoxia could affect the metastasis-free survival of patients with prostate cancer, and a hypoxia-related gene signature composed of seven genes for predicting metastasis was established and verified in different cohorts. The study further evaluated the effects of ALDOB expression on the proliferation and invasion of the LNCaP and DU145 cell lines under hypoxia and finally constructed a nomogram containing specific clinical characteristics of prostate cancer combined with the hypoxia gene signature to quantify the metastasis risk of individual patients.

Results: The hypoxia-related gene signature was identified as an independent risk factor for metastasis-free survival in patients with prostate cancer. The expression of ALDOB increased under hypoxia and promoted the proliferation and invasion of LNCaP and DU145 cells. In addition, patients with a high risk score showed therapeutic resistance and immunosuppression. Compared with other parameters, the nomogram had the strongest predictive power and net clinical benefit.

Conclusion: The study established a hypoxia-related gene signature and a nomogram to distinguish and predict patients with a high risk of prostate cancer metastasis, which may help to optimize individualized treatment and explore possible therapeutic targets.
The Potential Use of Cannabis in Tissue Fibrosis
Authors: Pryimak Nazar , Zaiachuk Mariia , Kovalchuk Olga , Kovalchuk Igor
Published: October 2021

Fibrosis is a condition characterized by thickening or/and scarring of various tissues. Fibrosis may develop in almost all tissues and organs, and it may be one of the leading causes of morbidity and mortality. It provokes excessive scarring that excels the usual wound healing response to trauma in numerous organs. Currently, very little can be done to prevent tissue fibrosis, and it is almost impossible to reverse it. Anti-inflammatory and immunosuppressive drugs are among the few treatments that may be efficient in preventing fibrosis. Numerous publications suggest that cannabinoids and extracts of Cannabis sativa have potent anti-inflammatory and anti-fibrogenic properties. In this review, we describe the types and mechanisms of fibrosis in various tissues and discuss various strategies for prevention and dealing with tissue fibrosis. We further introduce cannabinoids and their potential for the prevention and treatment of fibrosis, and therefore for extending healthy lifespan.
Non-immune Cell Components in the Gastrointestinal Tumor Microenvironment Influencing Tumor Immunotherapy
Authors: Li Zhengshuo , Zhang Xiaoyue , Liu Can , Ma Jian
Published: October 2021

Interactions of genetic susceptibility factors, immune microenvironment, and microbial factors contribute to gastrointestinal tumorigenesis. The suppressive immune microenvironment reshaped by the tumors during gastrointestinal tumorigenesis directly contributes to T-cell depletion in tumor immunotherapy. Soluble factors secreted by tumor cells or stromal cells collectively shape the suppressive immune environment. Here, we reviewed the key factors in the gastrointestinal tumor microenvironment that influence tumor immunotherapy, focusing on the effects of fibroblasts, neuronal cells, soluble cytokines, exosomes, and the microbiome in tumor microenvironment. Research in this field has helped to identify more precise and effective biomarkers and therapeutic targets in the era of tumor immunotherapy.
A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque Rupture
Authors: Vinci Ramona , Pedicino Daniela , Bonanni Alice , D’Aiello Alessia , Severino Anna , Pisano Eugenia , Ponzo Myriana , Canonico Francesco , Ciampi Pellegrino , Russo Giulio , Di Sario Marianna , Montone Rocco Antonio , Trani Carlo , Conte Cristina , Grimaldi Maria Chiara , Cribari Francesco , Massetti Massimo , Crea Filippo , Liuzzo Giovanna
Published: October 2021

The evaluation of monocyte subset distribution among acute coronary syndrome (ACS) patients according to culprit coronary plaque morphology has never been explored. We evaluated whether there were significant differences in frequency of circulating monocyte subsets isolated from ACS patients according to optical coherence tomography (OCT) investigation of plaque erosion and rupture. We enrolled 74 patients with non-ST-elevation ACS (NSTE-ACS), 21 of them underwent OCT investigation of the culprit coronary plaque and local macrophage infiltration (MØI) assessment. As control, we enrolled 30 chronic coronary syndrome (CCS) patients. We assessed the frequency of monocyte subsets in the whole study population, in reliance on their CD14 and CD16 expression (classical, CM: CD14++CD16–; intermediates, IM: CD14++CD16+; non-classical, NCM: CD14+CD16++). Then, we tested the effect of lipopolysaccharide (LPS) (a CD14 ligand) on peripheral blood mononuclear cells (PBMCs) of NSTE-ACS patients, quantifying the inflammatory cytokine levels in cell-culture supernatants. Our data proved that monocyte subsets isolated from NSTE-ACS patients represent a peculiar biological signature of the pathophysiological mechanism lying beneath atherosclerotic plaque with a ruptured fibrous cap (RFC) as compared with plaque erosion. Moreover, the magnitude of LPS-mediated effects on IL-1β, IL-6, and IL-10 cytokine release in cell-culture supernatants appeared to be greater in NSTE-ACS patients with RFC. Finally, we described a fourth monocyte population never explored before in this clinical setting (pre-classical monocytes, PCM: CD14+CD16–) that was prevalent in NSTE-ACS patients as compared with CCS and, especially, in patients with RFC and culprit plaque with MØI.
Cytotoxicity, Antimicrobial, and In Silico Studies of Secondary Metabolites From Aspergillus sp. Isolated From Tecoma stans (L.) Juss. Ex Kunth Leaves
Authors: Elsayed Heba E. , Kamel Reem A. , Ibrahim Reham R. , Abdel-Razek Ahmed S. , Shaaban Mohamed A. , Frese Marcel , Sewald Norbert , Ebrahim Hassan Y. , Moharram Fatma A.
Published: October 2021

Endophytes are prolific producers of privileged secondary metabolites with diverse therapeutic potential, although their anticancer and antimicrobial potential still have a room for further investigation. Herein, seven known secondary metabolites namely, arugosin C (1), ergosterol (2), iso-emericellin (3), sterigmatocystin (4), dihydrosterigmatocystin (5), versicolorin B (6), and diorcinol (7) were isolated from the rice culture of Aspergillus sp. retrieved from Tecoma stans (L.) Juss. ex Kunth leaves. Their anticancer and antimicrobial activities were evaluated in MTT and agar well diffusion assays, respectively. The cytotoxicity results showed that metabolite 3 displayed the best viability inhibition on the MCF-7 breast cancer cells with IC50 = 225.21 µM, while 5 on the HepG2 hepatocellular carcinoma cells with IC50 = 161.81 µM. 5 demonstrated a 60% apoptotic mode of cell death which is virtually correlated to its high docking affinity to Hsp90 ATP binding cleft (binding score −8.4 Kcal/mol). On the other side, metabolites 4 and 5 displayed promising antimicrobial activity especially on Pseudomonas aeruginosa with MIC = 125 μg/ml. The observed effect may be likely related to their excellent in silico inhibition of the bacterial DNA-gyrase kinase domain (binding score −10.28 Kcal/mol). To the best of our knowledge, this study is the first to report the promising cytotoxic and antibacterial activities of metabolites 3, 4, and 5 which needs further investigation and renovation to therapeutic leads.
Clinical and Immunological Characteristics of Patients With Adenovirus Infection at Different Altitude Areas in Tibet, China
Authors: Wang Bowen , Peng Mengjia , Yang Li , Li Guokai , Yang Jie , Yundan Ciren , Zeng Xiaohua , Wei Qianqi , Han Qi , Liu Chang , Ding Ke , Peng Kaige , Kang Wen
Published: October 2021

Background

The severities of human adenovirus (HAdV) infection are diverse in different areas of Tibet, China, where a large altitude span emerges. Serious consequences may be caused by medical staff if the clinical stages and immunological conditions of patients in high-altitude areas are misjudged. However, the clinical symptoms, immunological characteristics, and environmental factors of HAdV infection patients at different altitude areas have not been well described.

Methods

In this retrospective, multicenter cohort study, we analyzed the data of patients who were confirmed HAdV infection by PCR tests in the General Hospital of Tibet Military Command or CDC (the Center for Disease Control and Prevention) of Tibet Military Command from January 1, 2019, to December 31, 2020. Demographic, clinical, laboratory, radiological, and epidemiological data were collected from medical records system and compared among different altitude areas. The inflammatory cytokines as well as the subsets of monocytes and regulatory T cells of patients were also obtained and analyzed in this study.

Results

Six hundred eighty-six patients had been identified by laboratory-confirmed HAdV infection, including the low-altitude group (n = 62), medium-altitude group (n = 206), high-altitude group (n = 230), and ultra-high-altitude group (n = 188). Referring to the environmental factors regression analysis, altitude and relative humidity were tightly associated with the number of infected patients (P < 0.01). A higher incidence rate of general pneumonia (45.7%) or severe pneumonia (8.0%) occurred in the ultra-high-altitude group (P < 0.05). The incubation period, serial interval, course of the disease, and PCR-positive duration were prolonged to various extents compared with the low-altitude group (P < 0.05). Different from those in low-altitude areas, the levels of IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1, TNF-α, TNF-β, and VEGF in the plasma of the ultra-high-altitude group were increased (P < 0.05), while the proportion of non-classical monocytes and regulatory T cells was decreased (P < 0.05).

Conclusions

The findings of this research indicated that patients with HAdV infection in high-altitude areas had severe clinical symptoms and a prolonged course of disease. During clinical works, much more attention should be paid to observe the changes in their immunological conditions. Quarantine of patients in high-altitude areas should be appropriately extended to block virus shedding.
Settling in: Reintroduced Persian Fallow Deer Adjust the Borders and Habitats of Their Home-Range During the First 5 Years Post Release
Authors: Maor-Cohen Mia , Bar-David Shirli , Dolev Amit , Berger-Tal Oded , Saltz David , Spiegel Orr
Published: October 2021

Translocated animals typically find themselves in a novel environment in which they must establish a home range in a manner that will maximize their fitness. We hypothesized that the initial establishment of a home range is followed by adjustments expressed as home range shifting, and occurs as familiarity with the landscape increases, until the home range is stabilized. We studied the process of home range shifting in 42 female Persian fallow deer (Dama mesopotamica) reintroduced into the Galilee, Israel over a period of 2–5 years. We used changes in the degree of home range overlap between consecutive years as an indicator of stabilization. We then compared how the mean percent cover of the key vegetation types (woodland, scrubland and open pastures) differed between the areas abandoned in the first year's home range and the areas added to the last year's home range relative to the first (using a weighted paired t-test). We also compared the distribution (using χ2 test of independence and Levene's test for homogeneity of variance) of %cover of the 3 vegetation types between the first and last year's home range. The average home range overlap increased over the 5 years following the first release. During the first-year post release, deer avoided open pastures and preferred woodland. In later years deer increase in the % open pastures (weighted t-test: p < 0.001) and decreased the % woodland cover (weighted t-test: p = 0.07) by abandoning areas with little open pasture and steeper terrain and moving into areas with more open pasture and moderate terrain. Variance of the cover types across individuals increased with time. We conclude that the home ranges of the reintroduced deer stabilized with time. The changes in vegetation and slope are driven by time-dependent changing needs reflecting a tradeoff between safety (refuge) and foraging. Our findings suggest that using the initially established home range to determine species preferences can create a misleading picture of what the optimal home range of the species really is. Individual variation in term of preferences can take a few years to be expressed due to the initial high-risk perceived by individuals in a novel environment.
Conditioned Taste Aversion as a Tool for Mitigating Human-Wildlife Conflicts
Authors: Snijders Lysanne , Thierij Nina M. , Appleby Rob , St. Clair Colleen C. , Tobajas Jorge
Published: October 2021

Modern wildlife management has dual mandates to reduce human-wildlife conflict (HWC) for burgeoning populations of people while supporting conservation of biodiversity and the ecosystem functions it affords. These opposing goals can sometimes be achieved with non-lethal intervention tools that promote coexistence between people and wildlife. One such tool is conditioned taste aversion (CTA), the application of an evolutionary relevant learning paradigm in which an animal associates a transitory illness to the taste, odor or other characteristic of a particular food item, resulting in a long-term change in its perception of palatability. Despite extensive support for the power of CTA in laboratory studies, field studies have exhibited mixed results, which erodes manager confidence in using this tool. Here we review the literature on CTA in the context of wildlife conservation and management and discuss how success could be increased with more use of learning theory related to CTA, particularly selective association, stimulus salience, stimulus generalization, and extinction of behavior. We apply learning theory to the chronological stages of CTA application in the field and illustrate them by synthesizing and reviewing past applications of CTA in HWC situations. Specifically, we discuss (1) when CTA is suitable, (2) how aversion can be most effectively (and safely) established, (3) how generalization of aversion from treated to untreated food can be stimulated and (4) how extinction of aversion can be avoided. For each question, we offer specific implementation suggestions and methods for achieving them, which we summarize in a decision-support table that might be used by managers to guide their use of CTA across a range of contexts. Additionally, we highlight promising ideas that may further improve the effectiveness of CTA field applications in the future. With this review, we aspire to demonstrate the diverse past applications of CTA as a non-lethal tool in wildlife management and conservation and facilitate greater application and efficacy in the future.
A Practical Approach to Combined Transcatheter Mitral and Tricuspid Valve Intervention
Authors: Burke Lucas , Hassanin Magdi , Ong Geraldine , Fam Neil
Published: October 2021

Concomitant tricuspid regurgitation (TR) is common in patients with mitral regurgitation (MR). While current guidelines recommend repair of both valves at the time of surgery when feasible, high risk patients are often undertreated, leading to significant morbidity and mortality. With advances in transcatheter edge-to-edge repair (TEER) devices and technique, combined TEER for treating significant MR and TR has emerged as a new tool for heart failure management. Recent evidence has shed light on which patients with severe TR should be targeted for transcatheter intervention either in isolation or in combination with a MV TEER procedure and allows for expanded treatment options in patients who otherwise would be limited to medical management. Technological advancements remain ahead of robust clinical data, and thus randomized clinical studies in patients with severe MR and TR will be instrumental in determining the best approach in treating these patients with transcatheter therapies.