bluuebunny/crossref_metadata_2025 huggingface dataset:
MENGKOMUNIKASIKAN SECARA KREATIF KEBIJAKAN DAN LAYANAN PERPUSTAKAAN MELALUI MEDIA SOSIAL
Authors: Isrowiyanti Isrowiyanti
Published: November 2019

Libraries as nonprofit institutions are required to continue to develop their services
even though sometimes they have to face very limited budget conditions. The existing
facilities and opportunities must be optimized so that the library program continues and
reaches as many users as possible. Information technology and telecommunications
are both opportunities and challenges for librarians in order to enforce the existence
of libraries amid the shifting patterns and lifestyles of librarians in addition to the
limitations possessed by libraries. This paper describes how library efforts utilize the
trend of virtual communication to communicate creatively library policies through
social media.
Keywords: creative communication, library policy, library services, social media
RIA SW DAN ARIEF MUHAMMAD VLOG SEBAGAI MEDIUM DISEMINASI INFORMASI DAN KREATIVITAS
Authors: Sari Fitri Kartika
Published: November 2019

The development of the internet has made it easier for people to get information,
spread information, and work using social media. Vlog is one of the social media
that can earn income if it is consistent in its use. This study aims to compare the way
of vloggers in delivering video blogs. This study used descriptive qualitative method.
The results of this study indicate that the vlog made by Ria SW with Arief Muhammad
has different content where they are both vloggers who are looking for income by
attracting people to see the videos they upload. Suggestions and recommendations
from this study are that anyone can disseminate their work to the wider community,
besides earning income by uploading videos such as Ria SW and Arief Muhammad if
the public wants to use the internet media well.
Keywords: vlog, social media, ria sw, arief Muhammad
SISTEM PENGARSIPAN ARSIP ELEKTRONIK
Authors: Salmin Tajrid
Published: November 2019

In the current modern era, manual filing systems are less popular because they are less practical so it is necessary to implement in the form of paperless office or digitalization. The advantages of electronic files are easily visible. There will always be a need for hard-copy printed material, if only to ‘feel’ holding a physical copy of a journal, but despite the possible losses caused by technological changes there are good reasons (including space, searching, access and permanence) to consider the electronic version to be more flexible and useful products.Documents or archives in the form of paper that are stored in offices or government institutions should be managed properly so that they benefit the organization. To keep it easier and safer, some actions are taken, including by melting, sorting, indexing, placing it into a folder and putting it into a filing cabinet. This applies also to electronic archives, which require several methods to manage them so that they are easy to understand and easy to find if needed so that they are useful both when needed or in the future.

Keywords: Archives, filing systems, electronic archives.
PERAN MAHASISWA PART TIME DALAM MANAJEMEN KOLEKSI DI PERPUSTAKAAN UNIVERSITAS ISLAM INDONESIA YOGYAKARTA
Authors: Al Husna Hana Isnaini
Published: November 2019

Writing in this paper aims to see further which role or expected role of part-time students or workers who calculate the time in collection management or collection development in the Directorate of Library UII Yogyakarta. This needs to be seen because there are still many universities in Indonesia that still lack librarians, while librarians themselves have been more involved in fields that can be termed managers. In this paper will be examined to what extent part-time students are involved in collection management, is it possible to develop it to assist in the collection policy domain. The method used in this study is qualitative by means of interviews and observations. Based on observations and interviews, the involvement of part time students in the collection management process in the UII Library Directorate is limited to assisting technical problems, for involvement in policies all handled by the leadership in this case is the Library Director and Division Head in the UII Library. In this research, the author sees that it can still be developed the participation of the involvement in the policy making process, as an example that part time students are also users so that their opinions or ideas can be raised from them to create a collection development policy that is flexible and user friendly.
PROBLEMATIKA KEBIJAKAN DALAM MENGEMBANGKAN KOLEKSI DI PERPUSTAKAAN PERGURUAN TINGGI
Authors: Dila Batriatul Alfa
Published: November 2019

The paper gives a view to librarians to find out the problem of policy in the process

of developing collections in high-rise library. This paper focuses on finding solutions

to policy problems in developing collections in high-education libraries, to determine

the success in developing library materials in the high-rise library. At the writing

of this paper the method is more specific by using a literature review. The steps that

must be carried out are as follows: 1. Urgency of collection development policies, 2.

Carrying out activities that have been deemed by the library, 3. Involving users in the

provision of voluntary collections, 4. Planning the selection of library materials to

be provided in the library. the process of procuring a collection of library materials

that is relevant to be provided in the library, 6. Always evaluating library materials to

support success in the development of library collections. Constraints and solutions

faced in developing collections, with the emergence of this paper provide new insights

/ new ideas in providing the best solutions for the stages in developing collections in

the high-rise library.
Surgical Stabilization of Rib Fractures in a Patient with Empyema
Authors: Allen Rebecca , Estroff Jordan , Sarani Babak
Published: November 2019

Case:

A patient sustained flail chest and diaphragmatic rupture with perforation of the stomach. She underwent gastrorrhaphy with chest lavage. She developed empyema and underwent decortication. Intraoperative cultures grew bacteria and yeast. She failed extubation because of pain despite maximal medical therapy. She underwent surgical stabilization of rib fractures (SSRF). Intraoperative cultures remained positive. She was extubated 9 days after SSRF. She was ultimately discharged to home with a total of 2 months of antibiotics and no need for plate removal.

Conclusion:

The presence of infection should not be considered a contraindication to SSRF in patients who are mechanical ventilation dependent due to flail chest.
Pelestarian Budaya Campursari dalam Program TVRI Jawa Timur
Authors: Sinduwiatmo Kukuh , Rahayu Sri
Published: November 2019

The purpose of this study is to find out the programing undertaken by TVRI East Java in preserving the campursari culture. To uncover these goals thoroughly and profoundly, this study used descriptive method and usedquantitative method. Based on the problem, the data collection technique that used by the researcher were interview, direct observation and documentation conducted by researcher at the site. Then, the data was analyzing critically on the basis of Morissan thinking, so it obtained deep meaning about that used by TVRI East Java in preserving campursari culture. Based on the result of this study, it found the programming strategy in TVRI East Jawa in preserving campursari culture that is in the form of planning strategy where TVRI East Java did the determination of ideas before the show of campursari aired and bumper selection that change every year, a production strategy whereby TVRI East Java has own self produced campurasri event as well as arranging the best possible crack of business, evacuation strategy of the program where TVRI East Java showed live campursari program in studio 1 who filled by campursari communities from various regions. Then, evaluation strategy where in each year would evaluate the programs so, campursari can be accepted by public.
FORMATIVE ASSESSMENT PRACTICES AMONG DISTANCE EDUCATION TUTORS IN GHANA
Authors: Amoako Isaac
Published: November 2019

This study examines formative assessment procedures commonly used by Distance Education tutors’ during facilitation of the course model content. A descriptive survey research design was employed for the study. Census method was used in selecting the study respondents (tutors’) who were hundred and fifty (150) in number. Self-developed questionnaire was used for the data collection after it has been pilot tested for its refinement. The study revealed that ‘observation’, ‘oral questioning’, ‘peer-assessment’, ‘student self-assessment’ and ‘tutor made test’ are the current formative assessment practices of course tutors of on-site Distance Education in Ghana. It was recommended that tutors must use a wide range of formative assessment practices and not to cling on a single one. Unique student characteristics warrant the use of multiple formative assessment technique.
The Middle Ground of Curriculum: History Teachers’ Experiences in Ghanaian Senior High Schools
Authors: Oppong Charles Adabo , Awinsong Moses Allor , Apau Stephen Kwakye
Published: November 2019

This study explores Ghanaian history teachers’ experiences of the "middle ground of curriculum; a crucial stage of curriculum negotiation and a process, according to  Harris (2002), that includes what “teachers individually and collectively perceived and enacted. . . prior to classroom implementation” The study employed the concurrent parallel design (Quan-qual). The researchers collected quantitative data from sixty history teachers in Cape Coast Metropolis through the census method. Six teachers were randomly selected from the sixty to participate in the qualitative phase of the study. The quantitative data was analysed descriptively (means and standard deviations) while the qualitative data was analysed based on emerging themes. The findings revealed that the history departments through departmental relation, subject conceptualisation and governance influence the ways in which teachers negotiate the formal curriculum prior to teaching. More specifically, the study established the interaction of these variables that shape history teachers’ decision-making on the middle ground of the curriculum. The study, therefore, showed that the internalisation of curriculum change is a dynamic process that is evidenced at all levels of curriculum change – the high ground, middle ground of the curriculum, and lower ground
Employee Voice Contexts and Teacher Retention in Remote Secondary Schools in Tanzania
Authors: Boniface Raymond Mwemezi
Published: November 2019

Retaining teachers in their work stations is influenced by many factors which are contextually explained. Teachers’ retention practices in Tanzania and most Sub-Saharan African (SSA) countries have been ineffective partly because of being monetary based. While ‘voicing’ is regarded as a more feasible strategy for retaining teachers in these countries, conditions which favour voicing over exiting a remote school particularly in the Tanzanian context have been not systematically mapped out. This article presents and discusses seven conditions, to include: empowering, listening and cooperative leadership; habitability; friendliness outside school environment; investment potentialities; a supportive and peaceful school working environment; life as a “challenge” mindset; as well as patriotism and profession commitment, which were found to favour voicing over exiting a remote school. The findings imply that there is a need to empower teachers to influence change and reforms that matter to them, increasing teachers sense of investment in schools they are posted and in the profession (social and financial capital), checking ‘who goes into the teaching profession and with what level of struggle’; improving school-level relationships including justice practices from leaders and management, positive co-workers exchanges; training teachers to become patriotic to the nation and be committed to the teaching profession; and the need to improve cooperation and understanding between schools and their surrounding communities.
Dynamics and deposition of sediment-bearing multi-pulsed flows and geological implication
Authors: Ho Viet Luan , Dorrell Robert M. , Keevil Gareth M. , Thomas Robert E. , Burns Alan D. , Baas Jaco H. , McCaffrey William D.
Published: November 2019

Previous studies on dilute, multi-pulsed, subaqueous saline flows have demonstrated that pulses will inevitably advect forwards to merge with the flow front. On the assumption that pulse merging occurs in natural-scale turbidity currents, it was suggested that multi-pulsed turbidites that display vertical cycles of coarsening and fining would transition laterally to single-pulsed, normally graded turbidites beyond the point of pulse merging. In this study, experiments of dilute, single- and multi-pulsed sediment-bearing flows (turbidity currents) are conducted to test the linkages between downstream flow evolution and associated deposit structure. Experimental data confirm that pulse merging occurs in laboratory-scale turbidity currents. However, only a weak correspondence was seen between longitudinal variations in the internal flow dynamics and the vertical structure of deposits; multi-pulsed deposits were documented, but transitioned to single-pulsed deposits before the pulse merging point. This early transition is attributed to rapid sedimentation-related depletion of the coarser-grained suspended fraction in the laboratory setting, whose absence may have prevented the distal development of multi-pulsed deposits; this factor complicates estimation of the transition point in natural-scale turbidite systems.
AMAFRICA, a patient-navigator program for accompanying lymphoma patients during chemotherapy in Ivory Coast: a prospective randomized study
Authors: KOFFI Kouassi Gustave , Silué DA , Laurent C , Boidy K , Koui S , Compaci G , Adeba ZH , Kamara I , Botty RP , Bognini A.S , Sanogo I , Despas F , Laurent G
Published: November 2019

Background Previous studies have indicated that accompanying socially undeserved cancer patients through Patient Navigator (PN) or PN-derived procedures improves therapy management and reassurance. At the Cancer Institute of Toulouse-Oncopole (France), we have implemented AMA (Ambulatory Medical Assistance), a PN-based procedure adapted for malignant lymphoma (ML) patients under therapy. We found that AMA improve adherence to chemotherapy and safety. In low-middle income countries (LMIC), refusal and abandonment were documented as major adverse factors for cancer therapy. We reasoned that AMA could improve clinical management of ML patients in LMIC. Methods This study was set up in the Abidjan University Medical Center (Ivory Coast) in collaboration with Toulouse. Hundred African patients were randomly assigned to either AMA or control group. Main criteria of judgment were refusal and abandonment of CHOP or ABVD chemotherapy. Results We found that AMA was feasible and has significant impact on refusal and abandonment. However, only one third of patients completed their therapy in both groups. No differences were noted in terms of complete response rate (CRR) (16% based on intent-to-treat) and median overall survival (OS) (6 months). The main reason for refusal and abandonment was limitation of financial resources. Conclusion Altogether, this study showed that PN may reduce refusal and abandonment of treatment. However, due to insufficient health care coverage, its ultimate impact on OS remains limited.
The efficacy and safety of electro-acupuncture for alleviating chemotherapy-induced peripheral neuropathy in colorectal cancer patients: study protocol for a single-blinded, randomized sham-controlled trial
Authors: Chan Kaiyin , Lui Louisa , Yu Kaling , Lau Kwongwai , Lai Manchi , Lau Waiwai , Ng Bacon , Zhong Linda LD , Bian ZhaoXiang
Published: November 2019

Background: Colorectal cancer is the most common cancer in Hong Kong. Oxaliplatin-based chemotherapy is a major first-line conventional therapy for advanced and metastatic colorectal cancer. However, oxaliplatin causes chemotherapy-induced peripheral neuropathy (CIPN). Acupuncture has long been used to alleviate limb numbness in Chinese Medicine Practice. This study aims to examine the efficacy and safety of acupuncture for alleviating CIPN in colorectal cancer patients in Hong Kong.
Methods/Design: This is a single-blinded, randomized, sham-controlled efficacy trial. Eighty-four eligible patients, who are Hong Kong Chinese aged ≥18 years diagnosed with colorectal cancer and undergoing oxaliplatin-based chemotherapy, will be randomized in a ratio of 1:1 to the electro-acupuncture group and the sham-controlled group. During a 12-week treatment period, patients in the electro-acupuncture group will undergo electro-acupuncture once a week from the first cycle of chemotherapy, while patients in the control group will receive sham-acupuncture, and the patients in both groups will be followed up for twelve weeks. The primary outcome measure is the Functional Assessment of Cancer Therapy/Gynecologic Oncology Group-Neurotoxicity (FACT/GOC-Ntx) questionnaire. The secondary outcome measures include numerical rating scale (NRS) for numbness/pain, vibration and light touch sense test, quality of life questionnaire-C30 of European Organization for Research and Treatment of Cancer (EORTC QLQ-C30) and Constitution of Chinese Medicine Questionnaire (CCMQ).
Discussion: The study will compare electro-acupuncture with sham acupuncture to explore the feasibility for electro-acupuncture in improving symptoms caused by chemotherapy-induced peripheral neuropathy.
Treatment outcomes and their trend among tuberculosis patients treated at peripheral health settings of Northern Ethiopia Between 2009-2014: a registry-based retrospective analysis
Authors: Abdulkader Mahmud , Aken Ischa van , Niguse Selam Sahle , Hailekiros Haftamu , Spigt Mark
Published: November 2019

Objective: Evidence on treatment outcomes and their trend analysis through a register based retrospective study have significant contributions in the improvement of a national tuberculosis program. This study was aimed at determining tuberculosis treatment outcomes and their trend analysis.
Results: A total of 3445 patient records were included. More than half, (58%) were males and the mean age was 33.88 ± 16.91 years (range: 0-90). From the total TB patients, 18.8% were HIV co infected. The treatment outcome of TB patients were 371 (10.8%) cured, 2234 (64.8) treatment completed, died 119 (3.5%) died, 9 (0.3%) failed, 178 (5.1%) defaulted and 534 (15.5%) were transferred out. The overall treatment success rate was 89.5%. When assessed on yearly basis, treatment success rate was 87% in year 2009-2010 to 92.8% in 2013-2014 with 6.67% change in the outcome indicator over the five years period. Among pulmonary TB, pulmonary negative TB and extra pulmonary TB, the rate of successful treatment outcome was 83.1% to 89%, 85.1% to 89.4%, and 87.4% to 92%, respectively in the year 2009-2010 to 2013-2014. The percentage of the overall successful treatment outcomes were significantly associated with the year of treatment (p=0.014).
Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography
Authors: Dong Jie , Ding Xiangzhen , Wang Sheng
Published: November 2019

Background: The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity. Results : The recombinant GFP was transiently expressed in an active form in agoinoculated N. benthamiana leaves by using Tobacco mosaic virus (TMV) RNA-based overexpression vector (TRBO). The yield of recombinant GFP was up to ~60% of total soluble proteins (TSP). Purification of recombinant GFP from the clarified lysate of N. benthaniana leaves was achieved by using an alcohol/salt aqueous two-phase system (ATPS) and following with a further hydrophobic interaction chromatography (HIC). The purification process takes only ~4 h and can recover 34.1 % of the protein. The purity of purified GFP was more than 95% and there were no changes in its spectroscopic characteristics. Conclusions: The strategy described here combines the advantages of both the economy and efficiency of plant virus-based expression platform and the simplicity and rapidity of environmentally friendly alcohol/salt ATPS. It has a considerable potential for the development of a cost-efficient alternative for production of recombinant GFP. Keywords: Green fluorescent protein, Plant virus, Transient gene expression, Aqueous two-phase system, Hydrophobic interaction chromatography
Screening of Cellulolytic Bacteria from Rotten Wood of Qinling (China) for Biomass Degradation and Cloning of Cellulases from Bacillus methylotrophicus
Authors: Ma Lingling , Lu Yingying , Yan Hong , Wang Xin , Yi Yanglei , Shan Yuanyuan , Liu Bianfang , Zhou Yuan , Lv Xin
Published: November 2019

Background
Cellulosic biomass degradation still needs to be paid more attentions as bioenergy is the most likely to replace fossil energy in the future, and more evaluable cellulolytic bacteria isolation will lay a foundation for this filed. Qinling Mountains have unique biodiversity, acting as promising source of cellulose-degrading bacteria exhibiting noteworthy properties. Therefore, the aim of this work was to find potential cellulolytic bacteria and verify the possibility of the cloning of cellulases from the selected powerful bacteria.
Results
In present study, 55 potential cellulolytic bacteria were screened and identified from the rotten wood of Qinling Mountains. Based on the investigation of cellulase activities and degradation effect on different cellulose substrates, Bacillus methylotrophicus 1EJ7, Bacillus subtilis 1AJ3 and Bacillus subtilis 3BJ4 were further applied to hydrolyze wheat straw, corn stover and switchgrass, and the results suggested that B. methylotrophicus 1EJ7 was the most preponderant bacterium, and which also indicated that Bacillus was the main cellulolytic bacteria in rotten wood. Furthermore, scanning electron microscopy (SEM) and X-ray diffraction analysis of micromorphology and crystallinity of wheat straw also verified the significant hydrolyzation. With ascertaining the target sequence of cellulase β-glucosidase (243 aa) and endoglucanase (499 aa) were successfully heterogeneously cloned and expressed from B. methylotrophicus 1EJ7, and which performed a good effect on cellulose degradation with enzyme activity of 1670.15±18.94 U/mL and 0.130±0.002 U/mL, respectively. In addition, based on analysis of amino acid sequence, it found that β-glucosidase were belonged to GH16 family, and endoglucanase was composed of GH5 family catalytic domain and a carbohydrate-binding module of CBM3 family.
Conclusions
Based on the screening, identification and cellulose degradation effect evaluation of cellulolytic bacteria from rotten wood of Qinling Mountains, it found that Bacillus were the predominant species among the isolated strains, and Bacillus methylotrophicus 1EJ7 performed best on cellulose degradation. Meanwhile, the β-glucosidase and endoglucanase were successfully cloned and expressed from Bacillus methylotrophicus for the first time, which provided new materials of both strain and the recombinant enzymes for the study of cellulose degradation and its application in industry.
Echocardiographic Assessment of Asymptomatic US Air Force Members with Early HIV Infection
Authors: Alvarado Gadiel Rafael , Usry Courtney , Gore Rosco , Watts James , Okulicz Jason
Published: November 2019

Objective

People living with HIV (PLHIV) are at increased risk for cardiovascular disease (CVD) and development of subclinical echocardiographic abnormalities. However, there is scant evidence of the echocardiographic changes that occur shortly after seroconversion. In this study we describe the echocardiographic evaluations of asymptomatic US Air Force members who were diagnosed with HIV infection and evaluated at the San Antonio Military Medical Center between September 1, 2015 and September 30, 2016.

Results

Patients (n=50) were predominantly male (96%), mostly African American (60%), with a mean age of 28 years. At HIV diagnosis, the mean viral load was 112,585 copies/mL and CD4 count was 551 cells/uL. All were found to have normal left ventricular systolic ejection fraction (EF) and global longitudinal strain (GLS) however evidence of right ventricular dilatation and left ventricular remodeling was observed in 7 (14%) and 13 (26%) patients, respectively. Subgroup analyses showed no significant differences in echocardiographic findings by HIV disease severity or CVD risk factors (p >0.05 for all).This study suggests that untreated HIV may have a low impact on the development of echocardiographic abnormalities shortly after seroconversion. Longitudinal studies are warranted to determine the optimal CVD risk assessment strategies for PLHIV.
Clinical and ultrasonographic evaluation of shoulder in elite waterpolo players a cross-sectional study
Authors: Ruberti Andrea , Ronga Mario , Callegari Leonardo
Published: November 2019

Background: Rotator cuff structural changes are common in overhead throwing athletes, such as baseball, javelin, swimming, volleyball, by increasing the risk of shoulder pain and injury. The structural alterations in elite waterpolo players are not well known. In elite waterpolo athletes there are structural alterations of rotato cuff tendons due to overuse of the dominant shoulder compared to the non-dominant, like in oth er overhead disciplines, identifiable by ultrasound (US).
Methods: Asymptomatic elite men waterpolo players were selected. All athletes were evaluated with standardized scales of shoulder pain and function (Oxford Shoulder Score, Constant Shoulder Score) and provided data of training, injury and shoulder pain history. Each athlete underwent clinical evaluation of shoulders followed by US of rotator cuff, biceps and bursas.
Results : 23 athletes met the inclusion criteria, mean age 24.2±3.3. 14 athletes (60.9%) presented higher degeneration grade at the dominant supraspinatus compared to the contralateral, always in the same area in outfield pla yers: anterior, lateral, pre-insertional. In 22/23 (95.6%) the biceps tendon was damaged: 20/23 athletes (87%) bilaterally, 2/23 (8.7%) at the only dominant limb. The site was always at the bicipital groove in its insertion on the humerus. Power Doppler (PD) evidenced neovascularization in only 5/23 (21.7%) players at supraspinatus: 4 bilaterally, 1 only at the non-dominant. No statistical correlation was found between tendon degeneration and age, career years.
Conclusion: Outfield waterpolo players have peculiar changes in supraspinatus at preinsertional level, as seen in other overhead throwing athletes. We found changes also in biceps, bilaterally, like in shoulders of elite swimmers. We suppose that these changes are predictive of shoulder pain and injury.
Mutation at position 5628 in the mitochondrial tRNAAla gene in a Chinese pedigree with maternally diabetes mellitus
Authors: Li Ke , Wu Lijun , Lin Wei , Zhao Tianlan , Qi Qiang , Liu Jianjiang , Chen Weixin , He Wenjun , Liu Xiao , Shi Yuan
Published: November 2019

Background: To investigate the clinical, genetic and molecular characteristics of mitochondrial diabetes mellitus (MDM). Methods: Resultant variants were evaluated for evolutionary conservation, allelic frequencies, and structural and functional consequences. The mitochondrial function including mitochondrial tRNAAla levels, protein synthesis, membrane potential, adenosine triphosphate (ATP) production, and reactive oxygen species (ROS) generation were measured using lymphoblastoid cell lines carrying the m.5628T>C mutation and 2 controls .Results: We observed differences in the severity and age of onset in diabetes in affected maternally-related individuals, and through amolecular of the complete mitochondrial genome in this family, we identified a homoplasmic m.5628T>C mutation, located at conventional position 31 of tRNAAla, and we further detected distinct sets of mtDNA polymorphisms belonging to haplogroup L1. The identified mutation was further found be important for tRNA identity and stability. Using cellular models, we were able to determine that the respiratory deficiency caused arising as a consequence of the m.5628T>C mutation led to decreased efficiency of mitochondrial tRNAAla levels, protein synthesis, mitochondrial ATP synthesis and a reduced mitochondrial membrane potential.These mitochondrial dysfunctions caused an increase in the production of reactive oxygen species in the mutant cell lines. Conclusions: These data provide a direct evidence that novel m.5628T>C mutation may be associated with MDM, thus, offering novel insights into the understanding of pathophysiology of MDM.
Preliminary study of the use of a CBT screw to treat lumbar adjacent segment degeneration
Authors: Zhao Yonghui , Liang Jinlong , Luo Haotian , Ma Yulong , Chen Taibang , Teng Zhaowei , Peng Zhi , Xu Yongqing , Lu Sheng
Published: November 2019

Objective: To investigate the feasibility of using the cortical bone trajectory (CBT) screw in revision surgery for lumbar adjacent segment degeneration (LASD) and to provide a reference for clinical practice.Methods:The computed tomography (CT) scans of the lumbar spine of 40 patients in our hospital were used. Three-dimensional (3D) reconstruction was performed using Mimics 19.0 software, screws with appropriate sizes were selected for the L1 to L5 vertebral segments, and the traditional pedicle (TP) screws were placed using the standard method. After the completion of screw placement, the simulated placement of CBT screws with appropriate sizes was performed separately. During screw placement, the site and the direction were adjusted accordingly to complete the screw placement as much as possible. Under the premise of safe and feasible placement, penetration of the screw through the CBT and overlap between the TP and CBT screws should be avoided. If these requirements are met, screw placement is considered successful. Otherwise, the screw placement is considered failed. After all simulated screw placements were complete, the success rate of the screw placement in each segment of the lumbar vertebra was calculated from the statistical results. Finally, the pedicle trajectory reference width (TRW) was used for grouping to investigate the correlation between the TRW and sucess rate of screw placement. Results: Four hundred simulated screw placements (80 in each pedicle) were performed in the L1-L5 pedicles. The overall success rate of CBT screw placement in the lumbar spine was 57.00%. The success rates in the L1 to L5 segments were 47.50%, 62.50%, 57.50%, 70.00%, and 47.50%, respectively. The success rate in L4 was greater than in L2 and L3, while the lowest success rates were observed in L1 and L5. The groups were established based on the TRW. The success rate of CBT screw placement increased as the TRW increased.Conclusions: CBT screws are not suitable treatments for all cases of LASD. The success rate of screw placement varies in different lumbar segments. A preoperative measurement of TRW has important reference value for evaluating the feasibility of CBT screw placement in revision surgery for LASD.
The alteration of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) in the knee joints of osteoarthritis mice
Authors: Wang Luling , Zhang Demao , Sun Jianxun , Cui Yujia , Cai Linyi , Cui Chen , Lan Yuanchen , Gu Jinning , Liu Linfeng , Zhang Geru , Zheng Liwei , Pi Caixia , Xie Jing , Zhou Xuedong
Published: November 2019

Background: To clarify the expression and distribution of ADAMTS1, ADAMTS2 and ADAMTS5 in knee joints of osteoarthritis (OA) mice. Methods: OA was established via anterior cruciate ligament transection (ACLT) on the knee joints of C57BL/6J mice. The morphology change of OA was analyzed by Micro-CT. Histologic analysis was used to evaluate symptomatic change in articular cartilage and subchondral bone. Quantitative real-time PCR (qPCR) was used to analyze mRNA expressions of ADAMTS family in bone-related tissues and cells. Immunofluorescence staining was used to analyze the expressions and distributions of ADAMTS1, ADAMTS2, and ADAMTS5, as well as the condition of inflammation of OA.Results: Cartilage deterioration, significant reduction of collagen and proteoglycan components in the cartilage matrix happened in ACLT-induced OA mice, along with increased inflammatory response and osteoclast activity. Among ADAMTS, the gene expression levels of ADAMTS1, ADAMTS2 and ADAMTS5 were ranked top 5 in cartilage/chondrocytes, osteogenic tissue/osteoblasts and cortical bone/osteocytes. After ACLT surgery, the expressions of ADAMTS1, ADAMTS2 and ADAMTS5 all increased in articular cartilage, growth plate and subchondral bone of knee joints. Conclusion: The enhanced expressions of ADAMTS1, ADAMTS2 and ADAMTS5 after ACLT surgery provide a further understanding in degenerative change of OA.
Predicting Fluid Responsiveness Using Pulse Pressure Variation after Tidal Volume Challenge in Postoperative Patients Receiving Lung Protective Ventilation: A Clinical Trial
Authors: Kunakorn Pimsai , Morakul Sunthiti , Petnak Tananchai , Singhatat Pongsasit , Pisitsak Chawika
Published: November 2019

Background: Lung protective ventilation with low tidal volume is beneficial in patients with intermediate to high risk of postoperative pulmonary complications. However, during low tidal volume ventilation, pulse pressure variation (PPV) and stroke volume variation (SVV) do not predict fluid responsiveness. We aimed to determine whether changes in PPV and SVV after transient increases in tidal volume can predict fluid responsiveness in these patients. Methods: We recorded 22 measurements from 15 patients who experienced postoperative acute circulatory failure. We performed a tidal volume challenge by transiently increasing tidal volume (VT) from 6 to 8 mL/kg (VT6–8), 8 to 10 mL/kg (VT8–10), and 6 to 10 mL/kg (VT6–10) of patients' predicted body weight. The change in PPV (∆PPV) at VT6–8 (∆PPV6–8), VT8–10 (∆PPV8–10), VT6–10 (∆PPV6–10) and the change in SVV (∆SVV) at VT6–8 (∆SVV6–8), VT8–10 (∆SVV8–10), and VT6–10 (∆SVV6–10) were recorded. Patients were classified as fluid responders if there was an increase in stroke volume of more than 10% after a fluid bolus. Results: Following the tidal volume challenge, ∆PPV and ∆SVV failed to predict fluid responsiveness, with areas under the receiver operating characteristic curves (with 95% confidence intervals) of 0.49 (0.23–0.74), 0.54 (0.29–0.79), 0.52 (0.28–0.77) for ∆PPV6–8, ∆PPV8–10, and ∆PPV6–10, and 0.55 (0.30–0.80), 0.55 (0.31–0.80), and 0.59 (0.34–0.84) for ∆SVV6–8, ∆SVV8–10, and ∆SVV6–10, respectively. Conclusions: Changes in PPV and SVV after the tidal volume challenge did not predict fluid responsiveness in postoperative patients with low tidal volume ventilation. Trial registration: This trial was registered with Clinicaltrials.in.th, TCTR20190808003.
Psychological resources in adolescence and the association with labour market participation in early adulthood: A prospective cohort study
Authors: Jensen Jacob Devantie , Andersen Johan Hviid , Winding Trine Nøhr
Published: November 2019

Background: Young adults at the age of 25-29 have the highest unemployment rate and are in great risk of labour market marginalization. Exclusion from the labour market may have negative individual consequences on mental and physical health and can lead to increasing societal expenditures due to social benefits. It is important to understand what factors determine or protect against early labour market marginalization. The aim of the present study was to investigate the association between psychological resources in adolescence and labour market participation in early adulthood, and whether the associations differed by gender. Methods: This prospective cohort study used questionnaire data collected through the West Jutland Cohort study in 2004 and 2007. The study population (N = 2,982) consisted of people born in 1989 and living in the county of Ringkjoebing at baseline in 2004. Outcome was dichotomized as +/- 12 months of passive labour market participation during the age of 25-29. Psychological resources were measured as self-esteem, sense of coherence and mastery. Logistic regression analyses were performed to investigate the associations between psychological resources and labour market participation. Results: Results indicated associations between high levels of mastery or sense of coherence in adolescence and high labour market participation in early adulthood. The strongest associations were observed for females with a medium (OR: 1.9) or high level (OR: 1.6) of mastery or a high level of sense of coherence (OR: 1.6) at age 15 and for males with a medium (OR: 2.7) or high (OR: 1.9) level of mastery or a high level of sense of coherence (OR:1.7) at age 18. The associations between adolescent self-esteem and labour market participation were modest at both ages. Conclusion: The results of the present study indicate associations between a high level of sense of coherence or mastery in adolescence and high labour market participation in early adulthood. Psychological resources seemed to play a bigger role for females in early adolescence compared to males, for whom a larger impact was seen in late adolescence. Further studies are needed to elaborate the properties of these observed differences by gender and period of adolescence.
Minority stress, depression, and cigarette smoking among Chinese gay versus bisexual men: A two-group structural equation model analyses
Authors: Li Jingjing , Huang Danqin , Windle Michael , Escoffery Cam , Wang Wei , Li Xiaoyan , Tao Kevin , Haardörfer Regine , Li Shiyue , Berg Carla J , Yan Hong
Published: November 2019

Background: Literature in the West suggested that bisexual men have a higher smoking rate compared to gay men. Data on patterns of smoking among gay and bisexual men are limited in Eastern countries like China. This study examined the cigarette smoking prevalence for gay versus bisexual men in China and their unique minority stress - smoking pathways. Methods: Between September 2017 and November 2018, we surveyed a convenience sample of 538 gay men and 138 bisexual men recruited from local sexual minority organizations in four metropolitan cities in China (i.e., Beijing, Wuhan, Nanchang, and Changsha). Measures included sexual orientation, sociodemographics, theory-based minority stressors, depressive symptoms, and past 30-day cigarette smoking. Two-group (gay men vs. bisexual men) structural equation modeling (SEM) was used to test possible distinct mechanisms between theory-based stressors, depressive symptoms, and cigarette smoking among gay men and bisexual men, respectively. Results: The average age of participants was 26.51 (SD = 8.41) years old and 76.3% of them had at least a college degree. Bisexual men reported a higher rate of cigarette smoking compared to gay men (39.9% vs. 27.3%). Two-group SEM indicated that the pathways for cigarette smoking were not different between gay and bisexual men. Higher rejection anticipation was associated with greater depressive symptoms (standardized β = 0.32, p < .001), and depressive symptoms was not associated with cigarette smoking. Conclusions: Minority stress, specifically rejection anticipation, may be critical considerations in addressing depressive symptoms, but not smoking, among both gay and bisexual men in China.
Differences in maternal mortality between urban and rural areas: Analysis of Maternal Mortality based in Jinan, China in 1995- 2018
Authors: Zhang Lihua , Zhen Tingting , Meng Qinyou , Yang Shimin , Pan Jiaxue , Wang Guoqun , Li Zhongliang
Published: November 2019

Background: Although a large number of studies have shown that maternal mortality in rural areas is higher than that in urban areas, few reports discussed about the detailed situation and the behind causes. Here, we summarized the maternal mortality from 1995 to 2018 in Jinan and the reason behind it was deeply discussed. It is expected to reveal the difference and its behind reasons, thus providing a basis for policy makers to develop interventions.
Methods: Data about maternal mortality in the selected city from 1995 to 2018 were collected through the local maternal and child health care network. Maternal death age, maternal death delivery location, maternal death location, number of pregnancies, number of deliveries and maternal death causes were analyzed. The composition ratio of above factors were compared in order to indicate the differences between rural areas and urban areas.
Results: The study showed that 75.34% of maternal deaths in urban areas occurred in tertiary hospitals, which were 2.13 times higher than that in rural areas(P<0.05). 16.67% of maternal deaths in rural areas delivered in primary hospitals, which were 12.17 times higher than that in urban areas (P<0.05) . The main cause of death in rural areas were attributed to direct obstetrics reasons, which were indirect obstetric reasons for urban areas (P<0.05). There was no difference of maternal deaths in the death age, number of births, and number of pregnancies between rural areas and urban areas.(P>0.05)
Conclusion: Policy makers should focus on the construction of medical institutions in rural areas, the improvement of rescue capabilities in rural areas and the convenience of transport in rural areas in order to narrow the gap between rural and urban areas.
Key words: Maternal Mortality; Differences between urban and rural areas; China
Significance of nuclear factor kappa beta activation on prostate needle biopsy samples in the evaluation of Gleason score 6 prostatic carcinoma indolence
Authors: Zupančič Marko , Pospihalj Boris , Cerović Snežana , Gazić Barbara , Drev Primož , Hočevar Marko , Perhavec Andraž
Published: November 2019

Background. Prostate cancer (PCa) is the most common cancer in men in developed European countries. Majority of men newly diagnosed with PCa are candidates for primary curative therapy, either with radical prostatectomy (RP) or radiation. However, many PCa are low risk, even indolent and these patients are candidates for active surveillance, so the prediction of such cancers is needed to avoid overtreatment. The main goal of our study was to find out whether the immunohistochemical expression of NF-κB p65 in biopsy samples with Gleason score 3+3=6 (GS 6) can be a negative predictive factor for PCa indolence. Methods. Study was based on a retrospective cohort of 178 PCa patients with initial total PSA ≤ 10 ng/ml, number of needle biopsy specimens ≥8, GS 6 on biopsy and T1/T2 estimated clinical stage who underwent laparoscopic radical prostatectomy and whose archived formalin-fixed and paraffin-embedded (FFPE) prostate needle biopsy specimens were used for additional immunohistochemistry staining for detection of NF-κβ p65. Both cytoplasmatic and nuclear NF-κB p65 expression in biopsy cores with PCa were correlated with postoperative pathological stage, positive surgical margins, GS and biochemical progression (BP) of disease. The final analysis involved 123 patients regarding the postoperative stage, surgical margins and GS and 118 regarding the BP. Results. Postoperative pathological stage 3 was noticed in 27 (22%) and positive surgical margins were detected in 13 patients (10,6%). After median follow-up of 66 months, BP (PSA ≥ 0,05 ng/ml) occurred in 20 (16,9%) patients, 11 (55%) with GS 6 after RP and 9 (45%) with GS 7. Cytoplasmatic nor nuclear NF-κB p65 expressions were not significantly associated with pathological stage, positive surgical margin and postoperative GS. Patients with positive cytoplasmic NF-kB reaction had significantly more BP compared to those with negative cytoplasmic NF-kB reaction with PSA 0,2 ng/ml as cutoff point (p=0,015) and a trend towards more BP with PSA ≥ 0,05 ng/ml as cutoff point (p=0,068). Conclusions. Cytoplasmic expression of NF-κB is associated with more BP and might be an independent prognostic factor for recurrence-free survival (RFS), but further studies including larger patient cohorts are needed to confirm these initial results.
Accumulation of AGO2 facilitates tumor proliferation and metastasis through upregulating Survivin, Vimentin and Snail in human hepatocellular carcinoma
Authors: Yang Yang , Mei Qi
Published: November 2019

Background：Argonaute 2 (AGO2), a typical member of the Ago gene family, plays a pivotal role in hepatocellular carcinoma (HCC) tumorgenesis through regulating the short interfering RNA-mediated gene silencing. However, the underlined mechanism needs clarified. Herein, we found that AGO2 was frequently upregulated in human HCC cancerous tissues compared with non-cancerous tissues.
Methods: Clinical analyses were performed to determine the relation between the expression level of AGO2 and prognosis in HCC patients. By using CRISPR/Cas9 approach in SMMC-7721 cells and establishing xenograft model in nude mice, we further identified the role of AGO2 in HCC. Gene expression microarray analysis was used to reveal the changes of gene expression profile mediated by AGO2 depletion in SMMC-7721 cells.
Results: We observed that the overexpression of AGO2 was associated with poor prognosis in HCC patients. The knockout of AGO2 inhibited tumor cell proliferation and metastasis in vivo and in vitro. We also identified that AGO2 facilitates HCC tumorigenesis through modulating Survivin, Vimentin and Snail expression.
Conclusions: Therefore, this study not only demonstrates that accumulation of AGO2 promotes cell proliferation and metastasis in HCC, but also provides a novel molecular mechanism in HCC progression.
RESEARCH OF ACOUSTIC MEASUREMENTS OF BENDING FORCE ON HIGH VOLTAGE INSULATORS
Authors: Debnar Tomas , Kubinec Peter , Micjan Michal
Published: November 2019

The aim of this research was to research and verify the possibilities of using microphone to determine mechanical load acting on high voltage insulator. The insulator features a cable hole, which can be used for optical cables. Let’s use this hole as a microphone mount in order to listen to sounds inside of an insulator. The cable hole and the insulator end holes were filled with insulating foam to insulate microphone from the external interferences. Acoustic measurements were performed on the outdoor post insulator C30-850-II. The insulator was placed in bending machine and was repeatedly mechanically stressed by bending forces. The results of this research verify the suitability of the selected method for future use in the proposed device for detection of mechanical overload of high-voltage insulators.