bluuebunny/crossref_metadata_2025 huggingface dataset:
While allied health students prefer face-to-face clinical placement, telehealth can support competency development: results from a mixed-methods study
Authors: Bacon Rachel , Hopkins Sian , Georgousopoulou Ekavi , Nahon Irmina , Hilly Catherine , Millar CaraJane , Flynn Allyson , Smillie Linda , Chapman Sarah , Brown Nicholas
Published: May 2023

Introduction

Student clinical placements are a mandatory requirement within most accredited health programs. During the COVID-19 pandemic, many health settings that had traditionally provided placements cancelled their offerings. Telehealth services however, increased and emerged as an alternative placement setting.

Aim

To compare the learning experiences for allied health students provided by telehealth and face-to-face accredited health placements.

Methods

Health students, from a university clinic between March to December 2020, delivering both face-to-face and telehealth consultations, were invited to complete a telephone survey with 3 demographic questions; and 10-items comparing their telehealth and face-to-face learning experiences. Pearson’s chi-squared/Fisher’s exact test was used to examine the association between each item and consultation setting. Qualitative survey data was thematically analysed using a descriptive approach.

Results

49 students from 2 universities and 5 disciplines completed the survey. Students rated their face-to-face experiences significantly higher than their telehealth experiences across all items (all p-values <0.01). Across 9 items students reported positive learning experiences in both settings. Students had greater opportunities to work in a multidisciplinary team in a face-to-face setting. Four themes were generated: (1) placements can vary in quality regardless of setting; (2) telehealth can provide valuable learning experiences and support competency development; (3) enablers for telehealth placements and (4) barriers for telehealth placements.

Conclusion

While telehealth can support student learning and competency development, in this study students preferred face-to-face experiences. To optimise telehealth placements consideration needs to be given to barriers and enablers such as technological issues and university curricula preparation.
The cellular response to ocean warming in Emiliania huxleyi
Authors: Dedman Craig J. , Barton Samuel , Fournier Marjorie , Rickaby Rosalind E. M.
Published: May 2023

Marine phytoplankton contribute substantially to the global flux of carbon from the atmosphere to the deep ocean. Sea surface temperatures will inevitably increase in line with global climate change, altering the performance of marine phytoplankton. Differing sensitivities of photosynthesis and respiration to temperature, will likely shift the strength of the future oceanic carbon sink. To further clarify the molecular mechanisms driving these alterations in phytoplankton function, shotgun proteomic analysis was carried out on the globally-occurring coccolithophoreEmiliania huxleyiexposed to moderate- (23°C) and elevated- (28°C) warming. Compared to the control (17°C), growth ofE. huxleyiincreased under elevated temperatures, with higher rates recorded under moderate- relative to elevated- warming. Proteomic analysis revealed a significant modification of theE. huxleyicellular proteome as temperatures increased: at lower temperature, ribosomal proteins and photosynthetic machinery appeared abundant, as rates of protein translation and photosynthetic performance are restricted by low temperatures. As temperatures increased, evidence of heat stress was observed in the photosystem, characterized by a relative down-regulation of the Photosystem II oxygen evolving complex and ATP synthase. Acclimation to elevated warming (28°C) revealed a substantial alteration to carbon metabolism. Here,E. huxleyimade use of the glyoxylate cycle and succinate metabolism to optimize carbon use, maintain growth and maximize ATP production in heat-damaged mitochondria, enabling cultures to maintain growth at levels significantly higher than those recorded in the control (17°C). Based on the metabolic changes observed, we can predict that warming may benefit photosynthetic carbon fixation byE. huxleyiin the sub-optimal to optimal thermal range. Past the thermal optima, increasing rates of respiration and costs of repair will likely constrain growth, causing a possible decline in the contribution of this species to the oceanic carbon sink depending on the evolvability of these temperature thresholds.
Elucidating the role of seed structure in the heterometallic seeded growth of copper-based nanocrystals
Authors: Jeong Soojin , Skalla Rebecca X. , Wang Yi , Zhu Baixu , Ye Xingchen
Published: May 2023

Seed-mediated synthesis is a versatile method to prepare multimetallic nanocrystals for diverse applications. However, many fundamental questions remain on how the structural and chemical properties of nanocrystal seeds control the reaction pathways, especially for nonaqueous synthesis at elevated temperatures. Herein, we elucidate the role of surface ligands and crystallinity of Au nanocrystal seeds on the heterometallic seeded growth of Cu-based nanocrystals. We found that weakly coordinating ligands are critical to facilitate the diffusion between Au and Cu, which enables subsequent one-dimensional growth of Cu. Replacing multiple-twinned Au seeds with single-crystalline ones switched the growth pathway to produce heterostructured nanocrystals. Our work illustrates the importance of precise control of seed characteristics for the predictive synthesis of structurally complex multimetallic nanocrystals.
Co-administration of extracellular matrix-based biomaterials with neural stem cell transplantation for treatment of central nervous system injury
Authors: Damle Eshan B. , Morrison Vivianne E. , Cioma Jozef , Volic Milla , Bix Gregory J.
Published: May 2023

Injuries and disorders of the central nervous system (CNS) present a particularly difficult challenge for modern medicine to address, given the complex nature of the tissues, obstacles in researching and implementing therapies, and barriers to translating efficacious treatments into human patients. Recent advancements in neural stem cell (NSC) transplantation, endogenous neurogenesis, and in vivo reprogramming of non-neural cells into the neuronal lineage represent multiple approaches to resolving CNS injury. However, we propose that one practice that must be incorporated universally in neuroregeneration studies is the use of extracellular matrix (ECM)-mimicking biomaterials to supply the architectural support and cellular microenvironment necessary for partial or complete restoration of function. Through consideration of developmental processes including neurogenesis, cellular migration, and establishment of functional connectivity, as well as evaluation of process-specific interactions between cells and ECM components, insights can be gained to harness and modulate native and induced neurobiological processes to promote CNS tissue repair. Further, evaluation of the current landscape of regenerative medicine and tissue engineering techniques external to the neurosciences provides key perspectives into the role of the ECM in the use of stem cell-based therapies, and the potential directions future neuroregenerative approaches may take. If the most successful of these approaches achieve wide-spread adoption, innovative paired NSC-ECM strategies for neuroregeneration may become prominent in the near future, and with the rapid advances these techniques are poised to herald, a new era of treatment for CNS injury may dawn.
Evaluation of autonomic nervous system activity in intradialytic hypotension using entropy and skin sympathetic nerve activity
Authors: Li Jiayi , Xing Yantao , Zhang Yike , Cui Chang , Wang Jing , Li Jianqing , Liu Chengyu
Published: May 2023

Introduction

The function of the autonomic nervous system (ANS) is crucial in the development of intradialytic hypotension (IDH). This study introduced the entropy of heart rate variability (HRV) and skin sympathetic nerve activity (SKNA) to provide a complementary nonlinear and dynamic perspective for evaluating ANS function concerning IDH.

Methods

93 patients undergoing hemodialysis (HD) were enrolled, and the baseline data, electrocardiogram (ECG), and SKNA were collected. The patients were separated into the IDH and nonIDH groups based on the thresholds, which were characterized as reductions in systolic blood pressure (SBP) of at least 20 mm Hg or mean arterial pressure (MAP) of at least 10 mm Hg. We developed a logistic regression model for IDH after analyzing the changes in the time domain, frequency domain, the entropy of HRV, and SKNA indices during HD.

Results

After 4-h HD, the detected results for heart rate, the ratio of low frequency and high frequency (LF/HF), and average SKNA (aSKNA) all increased in both groups. Nine out of the ten HRV indices and aSKNA in the nonIDH group were higher than those in the IDH group at most moments. aSKNA was positively correlated with heart rate (p = 0.0001) and LF/HF (p = 0.0005) in the nonIDH group, while the correlation disappeared in the IDH group, which indicated a worse ANS response in IDH patients. The logistic regression model exhibited the results of initial SBP [odds ratio (OR) 1.076; p = 0.001], and the difference between the last and first segments (DLF) of heart rate [OR 1.101; p =0.012] and LF/HF [OR 0.209; p =0.034], as well as the extreme value of the difference between other segments and the first segments (EOF) of aSKNA [OR 2.908; p =0.017], which were independent indicators for IDH.

Discussion

The new nonlinear and dynamic assessment perspectives provided by the entropy of HRV and SKNA help to distinguish differences in ANS patterns between IDH patients and nonIDH patients and have the potential to be used in clinical monitoring for HD patients.
Voxel-based morphometry reveals the correlation between gray matter volume and serum P-tau-181 in type 2 diabetes mellitus patients with different HbA1c levels
Authors: Gao Yian , Sui Chaofan , Chen Boyao , Xin Haotian , Che Yena , Zhang Xinyue , Wang Na , Wang Yuanyuan , Liang Changhu
Published: May 2023

Introduction

Emerging evidence suggested widespread decreased gray matter volume (GMV) and tau hyperphosphorylation were associated with type 2 diabetes mellitus (T2DM). Insulin resistance is one of the mechanisms of neuron degeneration in T2DM; it can decrease the activity of protein kinase B and increase the activity of glycogen synthesis kinase-3β, thus promoting the hyperphosphorylation of tau protein and finally leading to neuronal degeneration. However, the association between GMV and serum tau protein phosphorylated at threonine 181 (P-tau-181) in T2DM patients lacks neuroimaging evidence. We aimed to investigate the difference in brain GMV between T2DM patients with different glycated hemoglobin A1c (HbA1c) levels and healthy control (HC) subjects and the correlation between serum P-tau-181 and GMV in T2DM patients.

Methods

Clinical parameters, biochemical indicators, and MRI data were collected for 41 T2DM patients with high glycosylated hemoglobin level (HGL), 17 T2DM patients with normal glycosylated hemoglobin level (NGL), and 42 HC subjects. Voxel-based morphometry (VBM) method was applied to investigate GMV differences among groups, and multiple regression analysis was used to examine the correlation between serum P-tau-181 and GMV.

Results

Compared with HC subjects, the T2DM patients with HGL or NGL all showed significantly decreased GMV. Briefly, the GMV decreased in T2DM patients with HGL was mainly in the bilateral parahippocampal gyrus (PHG), right middle temporal gyrus (MTG), temporal pole (TPOmid), hippocampus (HIP), and left lingual gyrus. The GMV reduction in T2DM patients with NGL was in the right superior temporal gyrus (STG), and there was no significant difference in GMV between the two diabetic groups. The GMV values of bilateral PHG, right MTG, TPOmid, HIP, and STG can significantly (p < 0.0001) distinguish T2DM patients from HC subjects in ROC curve analysis. In addition, we found that serum P-tau-181 levels were positively correlated with GMV in the right superior and middle occipital gyrus and cuneus, and negatively correlated with GMV in the right inferior temporal gyrus in T2DM patients.

Conclusion

Our study shows that GMV atrophy can be used as a potential biological indicator of T2DM and also emphasizes the important role of P-tau-181 in diabetic brain injury, providing new insights into the neuropathological mechanism of diabetic encephalopathy.
Adherence to the Mediterranean diet and waist-to-hip ratio in middle-aged postmenopausal women are the main factors associated with semantic verbal fluency 12  years later
Authors: Rodriguez-Espinosa Norberto , Moro Miguel Adoración , Rodriguez-Perez Maria del Cristo , Almeida-Gonzalez Delia , Cabrera de Leon Antonio
Published: May 2023

Few studies have analized the effect of vascular risk factors and lifestyle habits affecting the middle age of postmenopausal women on later cognitive performance in old age. We have carried out an observational study to identify those factors and whether they differ from those acting in men. Postmenopausal women and males, both aged 40–60  years old at recruitment, from a community dwelling cohort were included. Data for this study were collected from the first visit at recruitment (2001 to 2005). Participants were interviewed with a questionnaire on their health-related antecedents and underwent a physical exam. The cohort was contacted again for a new presential visit between 2014 and 2015. A semantic verbal fluency test was included in this new visit protocol as a brief measure of cognition. Besides educational attainment, Mediterranean diet adherence 20th percentile (OR = 1.93; 95%CI = 1.07–3.47) and waist to hip ratio 80th percentile (OR = 1.81; 95%CI = 1.10–2,98) were the main factors associated to low semantic fluency performance in postmenopausal women, while declared diabetes mellitus (OR = 2.24; 95%CI = 1.16–4,33), HOMA 2 insulin resistance index (OR = 1.77; 95%CI =1.04–3,02), light physical activity in leisure time (OR = 0.41; 95%CI = 0.19–0,93) and recommended moderate to vigorous physical activity (OR = 2.09; 95%CI = 1.23–3.56) did in men. Factors in middle age that explain semantic verbal fluency in old age are different between postmenopausal women and men. Menopause related fat redistribution may be a precondition for other vascular risk factors. The effect of Mediterranean diet on cognition deserves new specific studies centered on postmenopausal women as group.
Non-insulin-based insulin resistance indices for predicting all-cause mortality and renal outcomes in patients with stage 1–4 chronic kidney disease: another paradox
Authors: Shen Feng-Ching , Lin Hugo You-Hsien , Tsai Wei-Chung , Kuo I-Ching , Chen Yi-Kong , Chao Yu-Lin , Niu Sheng-Wen , Hung Chi-Chih , Chang Jer-Ming
Published: May 2023

Non-insulin-based insulin resistance (IR) indices serve as the indicators of metabolic syndrome (MetS) but have limited value for predicting clinical outcomes. Whether the obesity paradox affects the predictive value of these indicators in patients with chronic kidney disease (CKD) remains unknown. We investigated whether MetS and non-insulin-based IR indices can predict all-cause mortality and renal outcomes in a prospective observational study with stage 1–4 CKD Asians (N = 2,457). These IR indices were associated with MetS. A Cox regression model including body mass index (BMI) revealed an association between MetS and renal outcomes. Among the IR indices, only high triglyceride–glucose (TyG) index was associated with adverse renal outcomes: the hazard ratio of Q4 quartile of the TyG index was 1.38 (1.12–1.70). All-cause mortality was marginally associated with MetS but not high IR indices. Low TyG and TyG–BMI indices as well as low BMI and triglyceride were paradoxically associated with increased risks of clinical outcomes. The triglyceride-to-high-density lipoprotein cholesterol ratio and metabolic score for IR indices were not associated with clinical outcomes. In conclusion, MetS and TyG index predict renal outcome and obesity paradox affects the prediction of IR indices in patients with stage 1–4 CKD.
Association of the android to gynoid fat ratio with nonalcoholic fatty liver disease: a cross-sectional study
Authors: Yang Ling , Huang Hangkai , Liu Zhening , Ruan Jiaqi , Xu Chengfu
Published: May 2023

Background

Nonalcoholic fatty liver disease (NAFLD) is becoming a severe global public health problem, and can developed into fibrotic nonalcoholic steatohepatitis (NASH), but its risk factors have not been fully identified. The objective of this study was to investigate the association between the android-to-gynoid fat ratio (A/G ratio) and the prevalence of NAFLD.

Methods

This cross-sectional study is based on the 2003–2006 and 2011–2018 cycles of the National Health and Nutrition Examination Survey and included 10,989 participants. Participants aged 20 and older without viral hepatitis or significant alcohol consumption were included. Dual-energy X-ray absorptiometry was used to assess body composition. NAFLD was diagnosed using the United States fatty liver index (US FLI). Multivariable logistic regression models were used to evaluate the association between the A/G ratio and NAFLD.

Results

The prevalence of NAFLD was 32.15% among the study population. Android percent fat and the A/G ratio were significantly higher in patients with NAFLD than in those without NAFLD [41.68% (0.25) vs. 32.80% (0.27), p < 0.001; 1.14 ± 0.01 vs. 0.94 ± 0.00, p < 0.001, respectively]. Logistic regression analysis showed that android percent fat was positively correlated to NAFLD (OR: 1.15, 95% CI: 1.11–1.18), while gynoid percent fat was negatively correlated to NAFLD (OR: 0.92, 95% CI: 0.90–0.94), and the A/G ratio was significantly associated with the prevalence of NAFLD (OR: 1.59, 95% CI: 1.38–1.82) and fibrotic NASH (OR: 2.01, 95% CI: 1.71–2.38). We also found that females had a notably diminished A/G ratio compared with males (0.91 vs. 1.12, p < 0.001). In addition, the female population proportion was negatively correlated with the A/G ratio, which may partly explain the lower prevalence of NAFLD in females. What is more, the OR value of the A/G ratio in the female subgroup was much higher than that in the male subgroup in all adjusted models.

Conclusion

A/G ratio is significantly associated with NAFLD and fibrotic NASH. Women have a lower A/G ratio than men, which may explain the sex difference in NAFLD prevalence. Furthermore, with a higher A/G ratio, the association between females and NAFLD are greatly elevated.
Establishment of a prognostic model for ovarian cancer based on mitochondrial metabolism-related genes
Authors: Meng Chao , Sun Yue , Liu Guoyan
Published: May 2023

Background

Mitochondrial metabolism and mitochondrial structure were found to be altered in high-grade serous ovarian cancer (HGSOC). The intent of this exploration was to systematically depict the relevance between mitochondrial metabolism-related genes (MMRGs) and the prognosis of HGSOC patients by bioinformatics analysis and establish a prognostic model for HGSOC.

Methods

First of all, screened differentially expressed genes (DEGs) between TCGA-HGSOC and GTEx-normal by limma, with RNA-seq related HGSOC sourced from The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) database. Subsequently, expressed MMRGs (DE-MMRGs) were acquired by overlapping DEGs with MMRGs, and an enrichment analysis of DE-MMRGs was performed. Kaplan-Meier (K-M) survival analysis and Cox regression analysis were conducted to validate the genes’ prognostic value, Gene Set Enrichment Analysis (GSEA) to elucidate the molecular mechanisms of the risk score, and CIBERSORT algorithm to explore the immuno landscape of HGSOC patients. Finally, a drug sensitivity analysis was made via the Drug Sensitivity in Cancer (GDSC) database.

Results

436 HGSOC-related DE-MMRGs (222 up-regulated and 214 down-regulated) were observed to participate in multiple metabolic pathways. The study structured a MMRGs-related prognostic signature on the basis of IDO1, TNFAIP8L3, GPAT4, SLC27A1, ACSM3, ECI2, PPT2, and PMVK. Risk score was the independent prognostic element for HGSOC. Highly dangerous population was characterized by significant association with mitochondria-related biological processes, lower immune cell abundance, lower expression of immune checkpoint and antigenic molecules. Besides, 54 drugs associated with eight prognostic genes were obtained. Furthermore, copy number variation was bound up with the 8 prognostic genes in expression levels.

Conclusion

We have preliminarily determined the prognostic value of MMRGs in HGSOC as well as relationship between MMRGs and the tumor immune microenvironment.
A retrospective study of autologous hematopoietic stem cell transplantation for peripheral T-cell lymphoma: pre-transplant patients with partial remission benefit from post-transplant maintenance therapy
Authors: Huang Zhenghua , Li Zhen , Wang Juan , Gui Ruirui , Zu Yingling , Yu Fengkuan , Lin Quande , Zhao Huifang , Zhang Yanli , Fang Baijun , Liu Yanyan , Zhou Keshu , Li Yufu , Fu Yuewen , Yao Zhihua , Song Yongping , Zhou Jian
Published: May 2023

Background

Whether autologous hematopoietic stem cell transplantation (ASCT) improves the survival of patients with peripheral T-cell lymphoma (PTCL) remains controversial. Some studies have demonstrated that the efficacy of ASCT is superior in patients with complete remission (CR), whereas patients with partial remission (PR) remain vulnerable to relapse after ASCT, resulting in decreased survival rates. Maintenance therapy after chemotherapy may reduce the relapse rate of PTCL and improve survival; however, the role of maintenance therapy after ASCT in PTCL remains unclear. In this study, we aimed to analyze the efficacy of ASCT and post-transplant maintenance therapy in PTCL.

Methods

We retrospectively analyzed the clinical data of 69 patients with PTCL who underwent ASCT at our center between November 2001 and November 2021. According to the patients’ intention, thirty patients received post-transplant maintenance treatment, whereas 39 did not. The overall survival (OS) and progression-free survival (PFS) between the groups were compared using the log-rank test.

Results

At a median follow-up of 36 months, the entire cohort’s 3-year OS and PFS were 67.8% and 53.0%, respectively. The 3-year OS and PFS of patients with CR1, CR2, and PR were 85.3% and 65.4%, 80.0% and 60.0%, and 38.4% and 32.0%, respectively (OS: P=0.001; PFS: P=0.003). The relapse rates between the groups with or without maintenance therapy were 26.7% vs. 52.2%, the 3-year OS was 86.0% vs. 54.2% (P=0.004), and the 3-year PFS was 73.3% vs. 37.5% (P=0.004). Further analysis revealed that the efficacy of maintenance therapy was not significant in patients with CR1 and CR2, whereas patients with PR benefited from maintenance therapy. The relapse rate of patients with PR who received or did not receive maintenance therapy was 33.3% vs. 78.7%, 3-year OS was 66.7% vs. 21.9% (P=0.007), and 3-year PFS was 66.7% vs. 12.5% (P=0.004).

Conclusions

Patients with CR in PTCL benefit from ASCT, and post-transplant maintenance therapy reduces the relapse rate and significantly improves OS and PFS in patients with PR.
Surgical treatment of postoperative intractable bile leakage after liver tumor surgery in children
Authors: Han Jianyu , Qin Hong , Yang Wei , Cheng Haiyan , Chang Xiaofeng , Zhu Zhiyun , Feng Jun , Yang Shen , Chen Yajun , Wang Huanmin
Published: May 2023

Aim

To summarize systematically our six-year experience in the surgical treatment of postoperative bile leakage after liver tumor surgery in children, and explore its reoperation approach and treatment effect.

Methods

The clinical data of 6 patients with postoperative bile leakage cured by surgery from January 2016 to January 2022 were reviewed retrospectively.

Results

Among the six pediatric patients with postoperative bile leakage cured by surgery, four were male (67%) and two were female (33%). All patients underwent complex segmentectomy. The median time to bile leakage was 14 days (range, 10 to 32), and the daily drainage volume was stable from 170 ml to 530 ml per day. After conservative treatment failed, four patients received biliary-enteric anastomosis (patients 1, 3, 4, and 6), and two patients received bilio-cholecyst anastomosis (patients 2 and 5). All six patients were successfully treated with reoperation, and five patients were alive and without recurrence, while one patient was lost to follow-up due to abandoned treatment.

Conclusion

Our study suggests that surgery is a reliable and effective treatment for postoperative intractable bile leakage in children undergoing complex segmentectomy. Bilioenteric anastomosis is the most common technique for bile leakage, and bilio-cholecyst anastomosis is a feasible and effective surgical approach. These findings have important implications for the management of postoperative complications in pediatric patients undergoing complex segmentectomy.
Ten-year experience with pharmacogenetic testing for DPYD in a national cancer center in Italy: Lessons learned on the path to implementation
Authors: Bignucolo A. , De Mattia E. , Roncato R. , Peruzzi E. , Scarabel L. , D’Andrea M. , Sartor F. , Toffoli G. , Cecchin E.
Published: May 2023

Background: Awareness about the importance of implementing DPYD pharmacogenetics in clinical practice to prevent severe side effects related to the use of fluoropyrimidines has been raised over the years. Since 2012 at the National Cancer Institute, CRO-Aviano (Italy), a diagnostic DPYD genotyping service was set up.

Purpose: This study aims to describe the evolution of DPYD diagnostic activity at our center over the last 10 years as a case example of a successful introduction of pharmacogenetic testing in clinical practice.

Methods: Data related to the diagnostic activity of in–and out-patients referred to our service between January 2012 and December 2022 were retrieved from the hospital database.

Results:DPYD diagnostic activity at our center has greatly evolved over the years, shifting gradually from a post-toxicity to a pre-treatment approach. Development of pharmacogenetic guidelines by national and international consortia, genotyping, and IT technology evolution have impacted DPYD testing uptake in the clinics. Our participation in a large prospective implementation study (Ubiquitous Pharmacogenomics) increased health practitioners’ and patients’ awareness of pharmacogenetic matters and provided additional standardized infrastructures for genotyping and reporting. Nationwide test reimbursement together with recommendations by regulatory agencies in Europe and Italy in 2020 definitely changed the clinical practice guidelines of fluoropyrimidines prescription. A dramatic increase in the number of pre-treatment DPYD genotyping and in the coverage of new fluoropyrimidine prescriptions was noticed by the last year of observation (2022).

Conclusion: The long path to a successful DPYD testing implementation in the clinical practice of a National Cancer Center in Italy demonstrated that the development of pharmacogenetic guidelines and genotyping infrastructure standardization as well as capillary training and education activity for all the potential stakeholders are fundamental. However, only national health politics of test reimbursement and clear recommendations by drug regulatory agencies will definitely move the field forward.
Role of the China South–South cooperation hegemonic strategy as an “early emulation” in a context of systemic chaos
Authors: Cabrera García Ada Celsa , Lo Brutto Giuseppe
Published: May 2023

This study seeks to contribute to the thesis that China is directing its efforts toward the construction of a set of institutions that are presented as an alternative interstate subsystem to the one that emerged in the second postwar period. In this research, we made progress in locating the main elements from which we prefigure one of the features of that project. This is the strategy that, based on the cooperation scheme implemented by China through the Belt and Road Initiative (BRI), the Global Development Initiative (GDI)1 and the Global Security Initiative (GSI),2 manifests itself in a kind of “early emulation” of the North American hegemonic strategy of the second postwar period. “Emulation” of the United States (US) is synthesized in a double process: first, in the way in which China is currently articulating an institutional framework under the intensification of the present systemic chaos, that is, in a previous or “early” moment with respect to that in which we could consider the clear rise of a new hegemonic power. This framework operates under the logic of a political dialog that allows trade agreements and promotes a development strategy based on structural change. Second, in a similar way to the multilateral consensus that underpinned the US project based on the promotion of “development” from the north to the south and with a fundamental role for cooperation and aid, China today deploys a similar argument promoting the scope of “a community of shared future”3 with its strategic partners and to which more and more states look to join, where the GDI and the GSI are fundamental axes.
Mindfulness meditation and bimanual coordination control: study of acute effects and the mediating role of cognition
Authors: Devillers-Réolon Louise , Temprado Jean-Jacques , Sleimen-Malkoun Rita
Published: May 2023

Introduction

Mindfulness meditation (MM) involves and benefits cognitive functioning, especially attention and inhibition processes, which are also implicated in the control of complex motor skills, such as bimanual coordination. Thus, MM practice could potentially enhance bimanual coordination control through its cognitive benefits. Accordingly, in this study, we investigated the acute effects of a brief MM session on bimanual coordination dynamics, attention, and inhibition abilities, as well as the mediation link between MM’s cognitive and motor improvements.

Methods

Healthy meditation-naïve (novices, n = 29) and meditation-experienced participants (meditators, n = 26) were randomly assigned to either an active control intervention (attentive listening to a documentary podcast) or a MM intervention (breathing and open monitoring exercise), both lasting 15 min. In the motor domain, pre- and post-tests assessed participants’ ability to intentionally maintain the anti-phase coordination pattern at maximal movement frequency and resist the spontaneous transition to the in-phase pattern. In the cognitive domain, the participants’ attentional, perceptual inhibition and motor inhibition abilities were assessed.

Results

Following both interventions, meditators and novices improved the stability of their anti-phase coordination pattern (p = 0.034, ηp2 = 0.10) and their attentional performance (p’s < 0.001, ηp2 > 0.40). Only following the MM intervention, meditators and novices improved their ability to intentionally maintain the anti-phase pattern by delaying or even suppressing the spontaneous transition to in-phase (p’s < 0.05, ηp2 ≥ 0.11), and improved concomitantly their motor inhibition scores (p = 0.011, ηp2 = 0.13). No effects were found on perceptual inhibition. The increase in motor inhibition capacities did not however statistically mediate the observed acute effects of MM on bimanual coordination control.

Conclusion

We showed that a single MM session may have acute benefits in the motor domain regardless of the familiarity with MM practice. Although these benefits were concomitant to enhanced attentional and motor inhibition abilities, no formal mediation link could be established between the observed motor and cognitive benefits. This study paves the way for the investigation of the mechanisms underlying MM effects on motor control, as well as longer-term benefits.
Socioemotional self- and co-regulation in functional seizures: comparing high and low posttraumatic stress
Authors: Roberts Nicole A. , Villarreal Lucia Dayana , Burleson Mary H.
Published: May 2023

Functional seizures (FS) are seizure-like symptoms without electroencephalogram (EEG)-based epileptic activity. Those with FS often show emotion-related dysfunction and disrupted interpersonal relationships, in which posttraumatic stress disorder symptoms (PTS) may play a role. We sought to better understand trauma comorbidities and socioemotional processes in FS, including affectionate touch, a form of social connection linked to emotion regulation and awareness. We administered questionnaires online to a community sample of 89 trauma-exposed FS participants (FS diagnoses were self-reported), 51 with and 38 without clinical-level PTS (FS-PTShi, FS-PTSlo) and 216 seizure-free matched trauma-exposed controls (TCs), 91 with and 125 without clinical-level PTS (TC-PTShi, TC-PTSlo) per the Posttraumatic Stress Disorder Symptom Checklist (PCL). As hypothesized, both FS-PTShi and FS-PTSlo reported more emotional avoidance (Brief Experiential Avoidance Questionnaire), more emotion regulation difficulties (Difficulties in Emotion Regulation Scale), and more perceived stress (Perceived Stress Scale) than PTS-matched counterparts. FS-PTShi also reported less reappraisal (Emotion Regulation Questionnaire), more loneliness (UCLA Loneliness Scale), and less frequent affectionate touch (Physical Affection Scale) during waking and surrounding sleep than TC-PTShi, whereas FS-PTSlo and TC-PTSlo did not differ. Neither FS group differed from PTS-matched controls in emotion suppression (Emotion Regulation Questionnaire) or comfort with social touch (Social Touch Questionnaire). Among FS, FS-PTShi reported more difficulties than FS-PTSlo on nearly all measures (non-significant trend for social support). Findings underscore potential synergistic effects of FS and PTS clinical symptoms in shaping experiences of one’s emotions and social world, suggesting fostering meaningful connections with others, including via affectionate touch, is an important treatment target.
The efficacy and cerebral mechanism of intradermal acupuncture for major depressive disorder: a study protocol for a randomized controlled trial
Authors: Wu Xiaoting , Tu Mingqi , Chen Nisang , Yang Jiajia , Jin Junyan , Qu Siying , Xiong Sangsang , Cao Zhijian , Xu Maosheng , Pei Shuangyi , Hu Hantong , Ge Yinyan , Fang Jianqiao , Shao Xiaomei
Published: May 2023

Background

Major depressive disorder (MDD) has emerged as the fifth leading cause of years lived with disability, with a high prevalent, affecting nearly 4% of the global population. While available evidence suggests that intradermal acupuncture may enhance the effectiveness of antidepressants, whether its efficacy is a specific therapeutic effect or a placebo effect has not been reported. Moreover, the cerebral mechanism of intradermal acupuncture as a superficial acupuncture (usually subcutaneous needling to a depth of 1–2 mm) for MDD remains unclear.

Methods

A total of 120 participants with MDD will be enrolled and randomized to the waiting list group, sham intradermal acupuncture group and active intradermal acupuncture group. All 3 groups will receive a 6-week intervention and a 4-week follow-up. The primary outcome will be measured by the Hamilton Depression Rating Scale-17 and the secondary outcome measures will be the Self-Rating depression scale and Pittsburgh sleep quality index. Assessments will be conducted at baseline, 3 weeks, 6 weeks, and during the follow-up period. In addition, 20 eligible participants in each group will be randomly selected to undergo head magnetic resonance imaging before and after the intervention to explore the effects of intradermal acupuncture on brain activity in MDD patients.

Discussion

If the intradermal acupuncture is beneficial, it is promising to be included in the routine treatment of MDD.

Clinical Trial Registration

Clinicaltrials.gov, NCT05720637.
Barriers to timely identification of bipolar disorder in youth: a multidimensional perspective
Authors: Keramatian Kamyar , Morton Emma
Published: May 2023

Bipolar disorder (BD) in youth often goes unrecognized and therefore untreated. However, little is known about pathways to treatment of youth with BD and factors that influence the time taken for each stage of these pathways. In this article, we use the conceptual framework by Scott and colleagues called the Model of Pathways to Treatment as a foundation to explore the components of delay in the diagnosis and treatment of youth with BD. The total time from the onset of symptoms until treatment initiation was divided into four sequential intervals; i.e., the Appraisal, the Help-seeking, the Diagnostic and the Pre-treatment intervals and potential disease, patient, and healthcare system/provider factors that influence each interval were identified. This multidimensional conceptual framework can offer a systematic approach to understanding and exploring barriers to early identification and interventions in BD, which is a crucial step in the development of strategies to facilitate prompt diagnosis and treatment. We hope this work contributes to the discussion on delayed diagnosis and treatment of youth with BD and provides a roadmap to inform future research studies and policy decisions.
The effects of a two-week neuromuscular intervention on biopsychosocial variables in people with patellofemoral pain: an observational study
Authors: Lack Simon David , Bartholomew Clare , North Thomas , Miller Stuart Charles , Neal Bradley Stephen
Published: May 2023

Introduction

Patellofemoral pain (PFP) is common and predominately affects active populations. Altered biomechanics and psychosocial variables have been reported in people with PFP, but the effects of neuromuscular exercise on these variables is unknown. We aimed to investigate changes in biopsychosocial measures following a two-week neuromuscular intervention in people with PFP.

Materials and Methods

We measured pain (visual analogue scale), function (Kujala), activity level (Tegner), psychological well-being (Orebro), lower-limb isometric strength (handheld dynamometry), three-dimensional (3D) lower limb kinematics, and surface electromyography (sEMG), in people with PFP. 3D lower-limb kinematics and sEMG were synchronously sampled during step-up, step-down, and overground running. All measures were repeated after participants had completed a two-week neuromuscular intervention consisting of three exercises completed once per day, five days per week.

Results

18 participants completed pre/post testing (60% females, mean age 30.6 years ±7.0, height 173.4cm ±10.4, mass 70.2kg ±12.4, symptom duration 39.0 months ±58.8), with three of 21 participants lost to follow-up. Across all clinical measures (muscle onsets, muscle activation and kinematics), the 95% bootstrapped confidence intervals (CI) of the mean difference contained the null hypothesis following the two-week neuromuscular intervention, indicating no significant differences.

Conclusion

A two-week neuromuscular intervention did not change biomechanical or psychosocial measures in people with PFP. Interventions with a longer duration or greater load magnitude are required to fully evaluate the biopsychosocial mechanisms of effect for exercise in people with PFP.
Development and application of a TaqMan-based real-time PCR method for the detection of the ASFV MGF505-7R gene
Authors: Qi Chuanxiang , Zhang Yongqiang , Wang Zhenzhong , Li Jinming , Hu Yongxin , Li Lin , Ge Shengqiang , Wang Qinghua , Wang Yingli , Wu Xiaodong , Wang Zhiliang
Published: May 2023

African swine fever virus (ASFV), the etiological agent of African swine fever (ASF), causes deadly hemorrhagic fever in domestic pigs. ASF's high mortality and morbidity have had disastrous effects on the world's swine industry. In recent years, the number of African swine virus strains has increased and presented new challenges for detecting classical ASFV-p72-based viruses. In this study, we observed that the ASFV MGF505-7R gene, a member of the multigene family that can enhance ASFV virulence and pathogenesis, has the potential to be a candidate for vaccine formulations. We also developed a real-time PCR assay based on the ASFV MGF505-7R gene and validated it in multiple aspects. The results indicated that the approach could detect standard plasmids with a sensitivity and a specificity of up to 1 × 101 copies/μL. Moreover, the assay had no cross-reactions with other porcine viruses. In laboratory and clinical settings, the assay can detect ASFV-infected samples at an early stage (4 hpi) and show a consistency of 92.56% when compared with classical ASFV detection in clinically ASFV-infected materials. This study's results also indicated that the TaqMan-based quantitative real-time PCR assay we developed for detecting the ASFV MGF505-7R gene is both sensitive and specific. This assay can provide a quick and accurate method for detecting ASFV and has the potential to be used as an optional tool for screening and monitoring ASF outbreaks.
Integrated evaluation of the requirements and excretions of Cu, Fe, Zn, and Mn for broilers via a uniform design method
Authors: Qiu Kai , Chen Zhimin , Chang Wenhuan , Zheng Aijuan , Cai Huiyi , Liu Guohua
Published: May 2023

This study aimed to determine the ideal balance profile of Cu, Fe, Zn, and Mn for broilers of 1–21 days of age via a uniform experimental design. In Experiment 1, 900 1-day-old Arbor Acres male broilers were randomly allotted to 15 dietary treatments with six replicates of 10 birds. A total of 14 experimental diets were formulated with the supplementation of 8~16, 123~160, 40~80, and 60~120 mg/kg of Cu, Fe, Zn, and Mn, respectively, in the basal diet, according to the uniform design method. The excretion of Cu, Fe, Zn, and Mn in the manure and the broiler performance were determined to build the ideal balance profile of these elements. Experiment 2 was conducted based on the ideal balance profile built in Experiment 1, to test its practicability using 720 broilers with two treatments. The dietary concentrations of Cu, Fe, Zn, and Mn in the control group were 15.19, 203.08, 76.78, and 86.13 mg/kg, respectively. In Experiment 1, the concentrations of Cu, Fe, Zn, and Mn in the diets were 16.96, 166.66, 46.01, and 60.26 mg/kg, respectively, when the average daily gain reached the optimum value. When the dietary concentrations of Cu, Fe, Zn, and Mn were 8.54, 130.66, 38.19, and 64.07 mg/kg, respectively, the total excretion of Cu, Fe, Zn, and Mn got the minimum value. There are corresponding ideal balance profiles for minimum excretion of a certain element. In Experiment 2, the dietary levels of Fe, Zn, and Mn were decreased by 17.93%, 40.08%, and 30.04%, respectively, which had no significant effect on average daily gain, average daily feed intake, and feed gain for 1~21 day-old broilers but markedly decreased the excretion of Cu and Mn and total excretion. It was concluded that there is a dilemma between growth performance and mineral excretion. Although dietary levels of Cu, Fe, Zn, and Mn supporting optimal growth are higher than those for minimizing mineral excretion, supplementing too many trace elements in the diets of broilers is unnecessary.
A System for the Automatic Detection and Evaluation of the Runway Surface Cracks Obtained by Unmanned Aerial Vehicle Imagery Using Deep Convolutional Neural Networks
Authors: Maslan Jiri , Cicmanec Ludek
Published: May 2023

The timely detection and recognizing of distress on an airport pavement is crucial for safe air traffic. For this purpose, a physical inspection of the airport maneuvering areas is regularly carried out, which might be time-consuming due to its size. One of the modern approaches to speeding up this process is unmanned aerial vehicle imagery followed by an automatic evaluation. This study explores the automatic detection of the transverse crack, its dimension measurement, and position determination within the slab on the concrete runway. The aerial image data were obtained from flights at the given altitude above the runway and processed using commercial multi-view reconstruction software to create a dataset for the training, verification, and testing of a YOLOv2 object detector. Once the crack was detected, the main features were obtained by image segmentation and morphological operations. The YOLOv2 detector was tuned with 3279 images until the detection metrics (average precision AP = 0.89) reached sufficient value for real deployment. The detected cracks were further processed to determine their position within the concrete slab, and their dimensions, i.e., length and width, were measured. The automated crack detection and evaluation system developed in this study was successfully verified on the experimental section of the runway as an example of practical application. It was proven that unmanned aerial vehicle imagery is efficient over broad areas and produces impressive results with the combination of artificial intelligence.
Safety and Efficacy of 2D Brachytherapy vs. 3D Image-Guided Adaptive Brachytherapy for Locally Advanced Cervical Cancer—A Single Institution Retrospective Study
Authors: Faye Mame Daro , Petruccelli Araujo Mariana , Wissing Michel D. , Alrabiah Khalid , Gilbert Lucy , Zeng Xing , Souhami Luis , Alfieri Joanne
Published: May 2023

Background: The treatment paradigm for locally advanced cervical cancer (LACC) has shifted from two-dimensional-brachytherapy (2D-BT) to three-dimensional-image-guided adaptive BT (3D-IGABT). In this retrospective study, we report our experience with the change from 2D-BT to 3D-IGABT. Methods: We reviewed 146 LACC patients (98 3D-IGABT and 48 2D-BT) who received chemoradiation between 2004 and 2019. The multivariable odds ratio (OR) for treatment-related toxicities and hazard ratios (HR) for locoregional control (LRC), distant control (DC), failure-free survival (FFS), cancer-specific survival (CSS) and overall survival (OS) are reported. Results: The median follow-up was 50.3 months. There was a significant decrease in overall late toxicities in the 3D-IGABT group compared to the 2D-BT group (OR 0.22[0.10–0.52]), late gastrointestinal (OR 0.31[0.10–0.93]), genitourinary (OR 0.31[0.09–1.01]) and vaginal toxicities (0% vs. 29.6%). Grade ≥ 3 toxicity was low in both groups (2D-BT: 8.2% acute, 13.3% late vs. 3D-IGABT: 6.3% acute, 4.4% late, NS). The five-year LRC, DC, FFS, CSS and OS for 3D-IGABT were 92.0%, 63.4%, 61.7%, 75.4% and 73.6%, compared to 87.3%, 71.8%, 63.7%, 76.3% and 70.8% for 2D-BT (NS). Conclusions: 3D-IGABT for the treatment of LACC is associated with a decrease in overall late gastrointestinal, genitourinary and vaginal toxicities. The disease control or survival outcomes were comparable to contemporary 3D-IGABT studies.