bluuebunny/crossref_metadata_2025 huggingface dataset:
COMPASS II—Coordination of Medical Professions Aiming at Sustainable Support Protocol for a feasibility study of cooperation between general practitioner practices and community care points
Authors: Peter Lisa , Stumm Judith , Wäscher Cornelia , Kümpel Lisa , Heintze Christoph , Döpfmer Susanne
Published: September 2022

Introduction

General practitioners (GP) increasingly face the challenge of meeting the complex care needs of multi-morbid patients. Previous studies show that GP practices would like support from other institutions in advising on social aspects of care for multi-morbid patients. Already existing counselling services, like community care points, are not sufficiently known by both GPs and patients. The aim of COMPASS II is to investigate the feasibility of cooperation between GP practices and community care points.

Methods and analysis

During the intervention, GPs send eligible multi-morbid patients with social care needs to a community care point. The community care points report the consultation results back to the GPs. In preparation for the intervention, in a moderated process, GP practices meet with the community care points to agree on information exchange. The primary outcome is the feasibility of the cooperation: Questionnaires will be sent to GPs, medical practice assistances and community care point personnel (focus: practicality, acceptability). Data will be collected on frequency and reasons for GP-initiated consultations at community care points (focus: demand). Qualitative interviews will be conducted with all participating groups (focus: acceptability, satisfaction). The secondary outcome is the assessment of changes in health-related quality of life, social support and satisfaction with care: participating patients complete a questionnaire before and three to six months after their counselling. The results of the study will be incorporated into a manual in which the experiences of the cooperation will be made available to other GP practices and community care points.

Discussion

In COMPASS II, GP practices establish cooperation with community care points. The latter are already existing institutions that provide independent and free advice on social matters. By using an existing institution, the established cooperation and experiences from the study can be used beyond the end of the study.

Trial registration

The trial is registered with DRKS-ID: DRKS00023798, Coordination of Medical Professions Aiming at Sustainable Support II.
Sex differences in saliva-based DNA methylation changes and environmental stressor in young African American adults
Authors: Saadatmand Forough , Abbas Muneer , Apprey Victor , Tailor Krishma , Kwabi-Addo Bernard
Published: September 2022

Background

Low socioeconomic status neighborhood exposure to stress and violence may be sources of negative stimuli that poses significant health risks for children, adolescents and throughout the life course of an individual. The study aims to investigate if aberrant epigenetic DNA methylation changes may be a potential mechanism for regulating neighborhood exposures and health outcomes.

Methods

Exposure to environmental stressors identified in 98 young African American (AA) adults aged 18–25 years old from the Washington D.C., area were used in the study. We correlated the association between stress markers; cortisol, CRP, IgG, IGA, IgM, and self-reported exposure to violence and stress, with quantitative DNA methylation changes in a panel of gene-specific loci using saliva DNA.

Results

In all participants studied, the exposure to violence was significant and negatively correlated with DNA methylation of MST1R loci (p = 0.032; r = -0.971) and nominally significant with NR3C1 loci (p = 0.053; r = -0.948). In addition, we observed significant and negative correlation of DNA methylation changes of LINE1 (p = 0.044; r = -0.248); NR3C1 (p = 0.017; r = -0.186); MSTR1 (p = 0.022; r = -0.192); and DRD2 (p = 0.056; r = -0.184; albeit nominal significant correlation) with IgA expression. On the other hand, we observed a significant and position correlation of DNA methylation changes in DRD2 (p = 0.037; r = 0.184) with IgG expression. When participants were stratified by sex, we observed in AA young male adults, significant DNA methylation changes of MST1R (p< 0.05) and association with exposure to violence and IgG level. We also observed significant DNA methylation levels of DRD2 (p< 0.05) and association with IgA, IgG, and cortisol level. Furthermore, we observed significant DNA methylation changes of NR3C1 (p< 0.05) with stress, IgA, and IgG in the male participants only. On the other hand, we only observed significant and a positive association of IgG with DNA methylation levels of ESR1 (p = 0.041) in the young AA female participants.

Conclusion

Our preliminary observation of significant DNA methylation changes in neuronal and immune genes in saliva samples supports our recently published genome-wide DNA methylations changes in blood samples from young AA male adults indicating that saliva offers a non-invasive means for DNA methylation prediction of exposure to environmental stressors in a gender-specific manner.
Little noticed, but very important: The role of breeding sites formed by bamboos in maintaining the diversity of mosquitoes (Diptera: Culicidae) in the Atlantic Forest biome
Authors: Müller Gerson Azulim , de Mello Cecilia Ferreira , Bueno Anderson S. , de Alcantara Azevedo Wellington Thadeu , Alencar Jeronimo
Published: September 2022

This study investigated the composition of mosquito species in different kinds of breeding sites in a tropical forest remnant of the Atlantic Forest and identified species of public health concern therein. Collections of immature forms of mosquitoes were carried out monthly at the Poço das Antas Biological Reserve in southeastern Brazil, between June 2014 and June 2015. Samples were collected from four types of breeding sites: bamboos, bromeliads, puddles, and a lake. A total of 1,182 specimens of mosquitoes belonging to 28 species and 13 genera were collected. Three species, Ad. squamipennis, An. neglectus, and Wy. arthrostigma represented 64.8% of the captured specimens. Only three species were found in more than one type of breeding site: Ps. ferox, An. triannulatus, and Tx. trichopygus. Two species of public health concern were found breeding in bamboo (Ae. aegypti and Ae. albopictus) and one in the lake (An. darlingi). Bamboo had the highest species richness, Shannon diversity, abundance of individuals and number of dominant species of all breeding sites. Similar Simpson diversity was obtained for bamboo and bromeliads, with higher values than those obtained for puddles and the lake. The significance of the four breeding sites, especially bamboos, is discussed in the context of controlling populations of sylvatic species of mosquitoes in Atlantic Forest areas.
Overlap between dengue, Zika and chikungunya hotspots in the city of Rio de Janeiro
Authors: Queiroz Eny Regina da Silva , Medronho Roberto de Andrade
Published: September 2022

Background

Arboviruses represent a threat to global public health. In the Americas, the dengue fever is endemic. This situation worsens with the introduction of emerging, Zika fever and chikungunya fever, causing epidemics in several countries within the last decade. Hotspot analysis contributes to understanding the spatial and temporal dynamics in the context of co-circulation of these three arboviral diseases, which have the same vector: Aedes aegypti.

Objective

To analyze the spatial distribution and agreement between the hotspots of the historical series of reported dengue cases from 2000 to 2014 and the Zika, chikungunya and dengue cases hotspots from 2015 to 2019 in the city of Rio de Janeiro.

Methods

To identify hotspots, Gi* statistics were calculated for the annual incidence rates of reported cases of dengue, Zika, and chikungunya by neighborhood. Kendall’s W statistic was used to analyze the agreement between diseases hotspots.

Results

There was no agreement between the hotspots of the dengue fever historical series (2000–2014) and those of the emerging Zika fever and chikungunya fever (2015–2019). However, there was agreement between hotspots of the three arboviral diseases between 2015 and 2019.

Conclusion

The results of this study show the existence of persistent hotspots that need to be prioritized in public policies for the prevention and control of these diseases. The techniques used with data from epidemiological surveillance services can help in better understanding of the dynamics of these diseases wherever they circulate in the world.
Predictors of knowledge and adherence to COVID-19 safety protocols among nurses at health facilities in Tamale Metropolis of Northern Ghana
Authors: Kanligi David Abatanie , Boah Michael , Adokiya Martin Nyaaba
Published: September 2022

Background

Corona Virus Disease of 2019 (COVID-19) emerged in 2019 and caused widespread disruption to many facets of life, including healthcare. Healthcare workers, particularly nurses, became the front-line fighters against the pandemic, making it imperative to comply with recommended safety protocols. However, many nurses were infected by the virus in the Tamale Metropolis, raising concerns regarding their level of adherence to the safety protocols. This study assessed the predictors of knowledge and adherence to COVID-19 safety protocols among nurses at selected health facilities in the Tamale Metropolis of northern Ghana.

Methods

A facility based cross-sectional study design was adopted and 339 nurses from six (6) public health facilities in the Tamale Metropolis were recruited for the study using questionnaires. The questionnaires were transformed into Google Forms for respondents to answer online via WhatsApp or email. The data were exported from the Google spreadsheet into SPSS and analyzed using descriptive and inferential statistics.

Results

Of the 339 participants, 60.2% were classified as having adequate knowledge while only 9.1% demonstrated high adherence to COVID-19 safety protocols. Knowledge of COVID-19 was predicted by source of information, and marital status, whereas health facility types predicted level of adherence. The odds of having adequate knowledge were higher among unmarried nurses (AOR = 1.94; 95% CI: 1.16–3.25; p = 0.012) and nurses using social media (AOR = 1.80; 95%CI 1.02–3.18; p = 0.042) compared to their counterparts. Meanwhile, primary health care nurses (AOR = 0.24; 95% CI = 0.12–0.50; p<0.001) and secondary health care nurses (AOR = 0.52; 95% CI = 0.31–0.88; P = 0.016) had reduced odds of exhibiting higher adherence compared to nurses from tertiary-level facility.

Conclusion

In this study, we found that knowledge was high but adherence to COVID-19 safety protocols was low. We suggest that facility managers should enforce compliance of their staff to the safety protocols to prevent spread of the virus within healthcare settings.
NMR-based metabolomic analysis identifies RON-DEK-β-catenin dependent metabolic pathways and a gene signature that stratifies breast cancer patient survival
Authors: Vicente-Muñoz Sara , Hunt Brian G. , Lange Taylor E. , Wells Susanne I. , Waltz Susan E.
Published: September 2022

Background

Advances in detection techniques and treatment have increased the diagnosis of breast cancer at early stages; however, recurrence occurs in all breast cancer subtypes, and both recurrent and de novo metastasis are typically treatment resistant. A growing body of evidence supports the notion that metabolic plasticity drives cancer recurrence. RON and DEK are proteins that promote cancer metastasis and synergize mechanistically to activate β-catenin, but the metabolic consequences are unknown.

Methods

To ascertain RON-DEK-β-catenin dependent metabolic pathways, we utilized an NMR-based metabolomics approach to determine steady state levels of metabolites. We also interrogated altered metabolic pathway gene expression for prognostic capacity in breast cancer patient relapse-free and distant metastasis-free survival and discover a metabolic signature that is likely associated with recurrence.

Results

RON-DEK-β-catenin loss showed a consistent metabolite regulation of succinate and phosphocreatine. Consistent metabolite alterations between RON and DEK loss (but not β-catenin) were found in media glucose consumption, lactate secretion, acetate secretion, and intracellular glutamine and glutathione levels. Consistent metabolite alterations between RON and β-catenin loss (and not DEK) were found only in intracellular lactate levels. Further pathway hits include β-catenin include glycolysis, glycosylation, TCA cycle/anaplerosis, NAD+ production, and creatine dynamics. Genes in these pathways epistatic to RON-DEK-β-catenin were used to define a gene signature that prognosticates breast cancer patient survival and response to chemotherapy.

Conclusions

The RON-DEK-β-catenin axis regulates the numerous metabolic pathways with significant associations to breast cancer patient outcomes.
Evolution of functional antibodies following acute Epstein-Barr virus infection
Authors: Karsten Christina B. , Bartsch Yannic C. , Shin Sally A. , Slein Matthew D. , Heller Howard M. , Kolandaivelu Kumaran , Middeldorp Jaap M. , Alter Galit , Julg Boris
Published: September 2022

While Epstein-Barr virus causes mostly asymptomatic infection, associated malignancies, and autoimmune and lymphoproliferative diseases occur. To dissect the evolution of humoral immune responses over the course of EBV infection and to gain a better understanding of the potential contribution of antibody (Ab) function to viral control, we comprehensively profiled Ab specificities and Fc-functionalities using systems serology and VirScan. Ab functions against latent (EBNA1), early (p47/54) and two late (gp350/220 and VCA-p18) EBV proteins were overall modest and/or short-lived, differing from humoral responses induced during acute infection by other viruses such as HIV. In the first year post infection, only p18 elicited robust IgM-driven complement deposition and IgG-driven neutrophil phagocytosis while responses against EBNA-1 were largely Fc-functionally silent and only matured during chronic infection to drive phagocytosis. In contrast, Abs against Influenza virus readily mediated broad Fc-activity in all participants. These data suggest that EBV evades the induction of robust Fc-functional Abs, potentially due to the virus’ life cycle, switching from lytic to latent stages during infection.
New perspectives on men’s underwear
Authors: Cole Shaun , Allan Jonathan A.
Published: September 2022

This editorial considers the position of men’s underwear in scholarship and popular culture, starting with a reflection on the enduring question aimed at men over the choice of boxers or briefs. It addresses the changes in attitudes and approaches to men’s underwear moving from functional to sensual and luxurious and ponders on future directions in the sustainability and design of men’s undergarments and the academic study of this topic. The editorial then speculates on new questions that could be considered in relation to men’s underwear and the ways in which the authors in this Special Issue address the consumption, representation, materiality and immateriality of men’s underwear.
DETERMINAN TINGKAT PENGANGGURAN DI PROVINSI BANTEN DAN ALTERNATIF KEBIJAKANNYA
Authors: Muhammad Ernanda  , Hutagaol Manuntun Parulian , Zulva Azijah
Published: September 2022

Penelitian ini bertujuan untuk menemukan dan memahami akar masalah dari tingginya tingkat pengangguran, serta merumuskan alternatif kebijakan yang perlu dilakukan oleh pemerintah terkait untuk memfasilitasi penurunan pengangguran di Provinsi Banten. Dengan memakai data sekunder periode waktu 2010-2019 yang didapatkan dari BPS, digunakan metode berupa analisis deskriptif dan analisis kuantitatif menggunakan persamaan regresi data panel dengan pendekatan Fixed Effect Model (FEM). Hasil penelitian ini menunjukkan hanya variabel PDRB sektor industri yang berpengaruh signifikan dalam menurunkan pengangguran, sedangkan variabel PDRB sektor pertanian, migrasi masuk, dan share tenaga kerja sektor industri menyebabkan kenaikan pengangguran secara signifikan. Sementara itu, variabel upah minimum, pertumbuhan ekonomi, dan share tenaga kerja sektor pertanian tidak berpengaruh terhadap tingkat pengangguran di Banten. Alternatif kebijakan yang dapat dilakukan untuk mengatasi masalah pengangguran di Banten yaitu: pengembangan industri pengolahan padat karya berbasis insentif di wilayah utara, pembangunan sektor pertanian berbasis teknologi dan infrastruktur di wilayah selatan, dan penciptaan lapangan kerja melalui pembangunan sektor informal.
MEMPREDIKSI GEJOLAK PERBANKAN DI INDONESIA DENGAN PENDEKATAN MARKOV SWITCHING VAR
Authors: Rusydiana Aam Slamet , Nurfalah Irfan , Laila Nisful
Published: September 2022

Penelitian ini bertujuan untuk mendeteksi indikator dini penyebab krisis perbankan konvensional dan perbankan syariah, mengidentifikasi periode terpanjang gejolak antara kedua perbankan serta membandingkan stabilitas antara keduanya. Metode yang digunakan yaitu pendekatan Markov Switching Vector Autoregressive (MS-VAR), dimana kelebihan pendekatan ini adalah nilai batas indeks krisis (threshold) merupakan variabel endogenous dengan kata lain periode distress dan lamanya gejolak merupakan bagian dari hasil estimasi. Leading indikator mikro untuk perbankan konvensional adalah cash ratio dan leading indicator makro untuk perbankan konvensional adalah interest rate. Sedangkan leading indikator mikro untuk perbankan syariah yaitu bank deposit dan cash ratio serta leading indicator makro untuk perbankan syariah yaitu interest rate, inflasi, kredit domestik, money supply dan current account/gdp. Z-score perbankan konvensional lebih tinggi (10.98) dari Z-score perbankan syariah (9.93) artinya secara umum perbankan konvensional lebih stabil dari perbankan syariah. Namun periode gejolak dialami perbankan konvensional yaitu sekitar Oktober 2014 – Januari 2016 sedangkan periode gejolak perbankan syariah sekitar Januari 2008 – Desember 2008.
DARURAT LITERASI MEMBACA DI KELAS AWAL:
Authors: Solihin Lukman
Published: September 2022

Dalam upaya membangun SDM yang berkualitas, Indonesia menghadapi masalah rendahnya mutu pembelajaran di berbagai jenjang pendidikan, khususnya kemampuan literasi membaca di kelas awal sekolah dasar. Padahal siswa yang tidak mampu membaca akan mengalami “efek Mathhew” berupa menurunnya motivasi belajar, rendahnya kemampuan menangkap informasi, berpotensi mengulang kelas, bahkan tidak melanjutkan pendidikan (drop out). Artikel ini berusaha mengkaji persoalan tersebut dan mendiskusikan solusinya. Kami menggunakan data lapangan dari beberapa daerah yang merupakan mitra program INOVASI, antara lain Kabupaten Bulungan, Lombok Utara, serta Sumba Barat Daya dan Sumba Timur. Data dikumpulkan pada medio 2019 melalui wawancara dan diskusi dengan guru, kepala sekolah, pengawas, serta fasilitator daerah pada program INOVASI. Analisis yang kami lakukan menunjukkan terdapat tiga persoalan utama, yaitu rendahnya kompetensi guru, kurikulum yang mengabaikan pelajaran membaca permulaan, serta minimnya sumber daya bacaan. Tiga persoalan ini perlu dipecahkan apabila kita berharap masa depan SDM Indonesia akan lebih berkualitas dan berdaya saing.
PENGEMBANGAN MATA PELAJARAN YANG BERKAITAN DENGAN UPAYA PENINGKATAN LITERASI BUDAYA DAN KEWARGANEGARAAN
Authors: Setiawan Budiana
Published: September 2022

AbstractMinistry of Education and Culture in 2016 has launched The National Literacy Movement, which has been carried out in schools, families and communities. The National Literacy Movement proposes six basic literacies, namely: language, numeracy, science, digital, finances, and culture and citizenship. In this case, cultural and citizenship literacy receives less attention because it is considered lack competitive value in face of 21st century global competition. Whereas cultural and citizenship literacy is a base for formation of five basic characters, which include: religious, nationalist, independent, integrity, and mutual cooperation. The problem is what subjects can increase student awareness, especially in junior high school in supporting cultural and citizenship literacy? What aspects can be contributed from the subjects? This study is qualitative with a desk research method. Results of the study shows that subjects that are considered to increase student awareness of five basic characters, include: Social Studies, Arts and Culture, and Education for Pancasila and Citizenship. Social studies encourage students to have social awareness and be able to live together in a pluralistic society. Art and Culture as a foundation to save diverse of arts and culture of Indonesian people in facing era of modernity. Education for Pancasila and Citizenship encourages students to understand and carry out their rights and obligations as citizens of Indonesia.
PERSEPSI PUBLIK TERHADAP PENULARAN PANDEMI KORONA DI INDONESIA KLUSTER GOWA
Authors: Kusno Ali
Published: September 2022

ABSTRAKPenelitian ini bertujuan untuk mengidentifikasi persepsi publik yang berkembang terhadap eks peserta Ijtima Ulama di Gowa yang dianggap sebagai salah satu kluster persebaran pandemi korona di Indonesia. Pendekatan penelitian menggunakan analisis wacana kritis Model Fairclough. Pendekatan itu memungkinkan penggunaan bahasa dalam wacana ditempatkan sebagai praktik sosial; wacana atau penggunaan bahasa dihasilkan dalam sebuah peristiwa diskursif tertentu; dan wacana yang dihasilkan berbentuk sebuah genre tertentu. Data penelitian  berupa  wacana tanggapan para pengguna Facebook terhadap pemberitaan tentang pandemi korona kluster Gowa. Teknik analisis data menggunakan model interaktif. Hasil penelitian menunjukkan bahwa setelah kegiatan Ijtima Ulama di Gowa, para eks peserta kegiatan tersebut mendapat beragam stigma negatif. sebagian besar warganet beranggapan bahwa eks kegiatan Gowa sebagai salah satu penyebar wabah korona di Indonesia. Meskipun para eks peserta kegiatan Gowa sudah menjalani proses karantina dan pengobatan, tetap mendapatkan perlakuan yang tidak mengenakkan dari masyarakat sekitar, terkesan eks kegiatan Gowa dan keluarga dikucilkan meskipun sebenarnya masyarakat lebih menarik diri. Pemahaman agama yang trerkesan membabi buta akan sangat berbahaya bagi kehidupan umat Islam. Penanganan lebih tegas terhadap kejadian serupa agar masyarakat agar lebih patuh terhadap kebijakan pemerintah.
Specialized Hospitals To Faced The Epidemic Beri-Beri On The War In Aceh
Authors: Yani Wahyu Suri
Published: September 2022

The epidemic beri-beri on the war in Aceh effect in high mortality on the Dutch army. The emergence of beri-beri as a contagious disease triggers the government to evacuate patients and establishing a special hospital in Sumatra Westkust. However, indigenous people never known and not infected the epidemic beri-beri. Treatment in special hospitals as a new epidemic became the main solution of the beri-beri problem which also had spread in various centers of the Dutch East Indies government. Through the evidence in Algemene Secreterie Atjeh Zaken, Geneeskundig Tijdschrift voor Nederlandsch Indies archive and other primary sources, indicate that the existence of specialized hospitals has solved the beri-beri problem. This historical research reconstructs the importance of the policy beri-beri hospitals and medical management to encounter of epidemics. The results also showed that discourse and topography of health in Sumatra Westkust with the locality approach became part of a long process to the discovery of anti-beri-beri or thiamine.
How People in the Remote Area React to the COVID-19 Pandemic in the Early Phase
Authors: Ghani Mochammad Wahyu
Published: September 2022

This article aims to analyze the knowledge and reaction of the remote area population which is the Silat Hilir District, Kapuas Hulu, West Borneo, at the beginning of the COVID-19 pandemic in Indonesia. The knowledge about COVID-19 is still limited so that access to valid information and reliable sources becoming very important. We conducted a participant observation to collect the data. It shows that the population of Silat Hilir District has a poor understanding related to COVID-19. They also tend to react to the COVID-19 hoaxes. Based on the observations which are supported by secondary data, variables like topography, lack of access to electricity and internet, and low level of education make the population of Silat Hilir District unable to process the information they received. Therefore, the development of infrastructure in remote area must also consider the increase of community literacy skills, for example through the education aspect.
Kebangkitan Dokter Pribumi dalam Lapangan Kesehatan : Melawan Wabah Pes, Lepra dan Influenza di Hindia Belanda Awal Abad 20
Authors: Hasanah Siti
Published: September 2022

Abstrak :Dalam upaya memutus mata rantai wabah dibutuhkan sinergitas yang kuat antara pemerintah pusat dan daerah, antara dokter dan masyarakat, maupun sesama dokter itu sendiri. Dalam tatanan birokrasi kesehatan kolonial, awalnya dokter pribumi selalu mengalami diskriminasi dan pada beberapa kasus hubungan dengan dokter Eropa tidak harmonis. Namun saat-saat terjadi wabah mengharuskan mereka tetap bersinergi. Awalnya dokter Eropa lebih dominan untuk menjadi tokoh-tokoh kunci dalam penelitian dan pencarian solusi ketika terjadi wabah. Lalu trendnya berubah sejak awal abad ke 19. Atas kebangkitan dokter pribumi yang tidak terlepas dari revolusi pendidikan STOVIA dan kemunculan Vereeniging van Inlandsche Geneeskundige, sebuah perkumpulan dokter pribumi di tahun 1909. Dua faktor ini mendorong para dokter pribumi semakin melibatkan diri dalam lapangan kesehatan maupun penelitian. Tulisan ini ingin menelusuri lebih jauh kebangkitan para dokter pribumi dalam lapangan kesehatan sejak awal abad 20, khususnya dalam konteks penanganan wabah di beberapa daerah. Lalu seperti apa sinergitas yang dibangun antara dokter pribumi dengan dokter Eropa dalam penanganan wabah, khususnya wabah pes, influenza dan lepra.Kata kunci : Kebangkitan, Dokter pribumi, Sinergitas, Wabah
Molecular age estimation based on promotor CpG methylation using Methylation Sensitive PCR
Authors: Le Clercq Louis-Stéphane IV , Dalton D. L. , Kotze A.
Published: September 2022

Chronological age is a key factor in animal ecology, as many biological traits appear and change over time. Such traits include development, age of reproductive maturity, reproductive success, future reproductive potential, and mortality. Several molecular methods have emerged as potential vehicle for biological age determination. The aim of this experiment is to ascertain if a Methylation Sensitive PCR (MSP) could be developed to screen for methylation at a previously identified site in the GRIA2 gene. Primers were designed by eye for both methylated and unmethylated target CpG’s in the GRIA2 promoter, and MSP conducted with the EpiScope® MSP kit. After optimization, the assay was able to successfully amplify the unmethylated control DNA with an efficiency of 97.6% and R²=99% across a range of 0.3–20 ng input DNA. Comparable results were, however, obtained with the methylated control DNA. Thus, the primers designed for the GRIA2 CpG was able to amplify the selected CpG with great efficiency making MSP a promising method of methylation screening, but primer design to assay a specific site faces many problems and selectivity for methylated vs. unmethylated may not be achievable.
Determinação da incerteza de medição nos ensaios de deformação permanente de solos lateríticos
Authors: Tanabe Assad dos Santos Juliana , Lopes da Silva Paulo Afonso , Rodrigues Guimarães Antonio Carlos
Published: September 2022

Durante um processo de análise laboratorial de solos, diversas variáveis influenciam na determinação do parâmetro pretendido. Inerentemente, cada uma das variáveis apresenta incertezas associadas, que podem estar relacionadas a fatores como calibração, leitura de dados, imperícia, etc. Diante do exposto, o presente artigo tem como objetivo determinar a incerteza das medições das deformações permanentes em solos lateríticos. Foram utilizados dados experimentais obtidos de ensaio triaxial de cargas repetidas realizados em areia argilosa do município de Mimoso do Sul, estado do Espírito Santo. A metodologia utilizada engloba as incertezas associadas à tensão confinante ( , à tensão de desvio ( ) e ao número de ciclos (N) , em geral informadas por certificado de calibração. As amostras foram divididas em dois grupos, diferenciados apenas pelo valor de  aplicado. Os resultados das medições de dois grupos de ensaio são expressos em forma de intervalo que inclui a incerteza calculada. Como resultado, pode-se observar que a incerteza de medição para o caso proposto não representa variação significativa do resultado para aplicação em pavimentos. Além disso, destaca-se a grande influência da tensão confinante na avaliação desse parâmetro ao comparar os resultados dos dois grupos. Concluiu-se que apresentar a incerteza das medições evidencia a confiabilidade e qualidade dos resultados.
Effects of Bowel Training and Defecation Posture on Chronic Constipation in Older Adults With Dementia: A Randomized Controlled Trial
Authors: Naito Tomoyoshi , Nakamura Mieko , Suzuki Mizue , Ojima Toshiyuki
Published: September 2022

INTRODUCTION:

Chronic constipation (CC), which can cause behavioral and psychiatric symptoms of dementia and related caregiver distress, is common in older adults admitted to care facilities with dementia. This study aimed to examine the effect of defecation care on CC and related problems.

METHODS:

This study compared bowel training and defecation posture intervention (intervention group) with general care (control group) as the treatment of CC among older adults with dementia in 6 long-term care facilities. The primary outcomes were the number of spontaneous bowel movements (SBMs) and complete SBMs. The secondary outcomes were Patient Assessment of Constipation Quality of Life Questionnaire, Constipation Scoring System, constipation symptoms, and Neuropsychiatric Inventory Nursing Home Version scores. The differences were analyzed using 2-way ANOVA with repeated measures.

RESULTS:

The data of 30 patients (14 in the intervention group, 16 in the control group) were analyzed. Weekly mean complete SBMs increased from 0.53 times at baseline to 1.58 times at 8 weeks in the intervention group compared with a change from 0.56 to 0.43 times in the control group (interaction P < 0.001). The Patient Assessment of Constipation Quality of Life Questionnaire, Constipation Scoring System, behavioral and psychiatric symptoms of dementia, and caregiver distress scores showed significant improvement after 8 weeks of defecation care intervention.

DISCUSSION:

Defecation care, including bowel training and appropriate defecation posture, is effective for CC among older adults with dementia, improving patient mental health and reducing burden on caregivers.
Long-Term Outcomes of Early vs Delayed Responders to Vedolizumab and Adalimumab: A Post Hoc Analysis of the VARSITY Study
Authors: Narula Neeraj , Wong Emily C.L. , Marshall John K. , Jairath Vipul , Dulai Parambir S. , Reinisch Walter
Published: September 2022

INTRODUCTION:

It is uncertain whether patients with ulcerative colitis (UC) and delayed symptomatic response to therapy have as robust and durable a response as earlier responders to therapy. We compared clinical outcomes of early and delayed responders to vedolizumab and adalimumab for patients with moderate-severe UC.

METHODS:

This was a post hoc analysis of the VARSITY study. Patients with early partial Mayo score (PMS) remission (PMS ≤1 at week 4/6 of therapy) were compared with those with delayed PMS remission (PMS ≤1 at week 14 and not week 4/6). Differences in proportions of patients achieving week 52 clinical remission (CR) (PMS = 0), endoscopic improvement (EI) (Mayo endoscopic subscore ≤1), and histoendoscopic mucosal improvement (HEMI) (Mayo endoscopic subscore ≤1 and Geboes score highest grade <3.2) were assessed. Confounders were adjusted for using multivariate logistic regression.

RESULTS:

A total of 147 vedolizumab-treated and 110 adalimumab-treated patients attained early or late PMS remission. Those who attained early PMS remission with vedolizumab were more likely to attain week 52 CR than participants with delayed PMS remission with vedolizumab (69.1% [67/97] vs 50.0% [25/50], aOR 2.43 [95% CI 1.11–5.33], P = 0.027). Week 52 HEMI was more likely among early vedolizumab PMS remitters (63.9% [62/97] vs 40.0% [20/50], aOR 2.60 [95% CI 1.20–5.62], P = 0.015). Week 52 EI was similar between early and delayed PMS remitters to vedolizumab. No differences were observed in week 52 CR, EI, or HEMI between early and delayed PMS remitters to adalimumab.

DISCUSSION:

Patients with UC who achieve early PMS remission with vedolizumab have greater odds of week 52 remission compared with delayed responders.
Pressing times, losing voice: critique and transformative spaces in higher education
Authors: Benswait Ahmad Jaber , Pérez-Milans Miguel
Published: September 2022

In this article, we examine our own doctoral supervisory dialogue as it has been institutionally interrupted due to Ahmad’s application for asylum in the UK. As we find ourselves lacking the conditions of recognisability required for our actions to be institutionally understood (or made intelligible) as part of a doctoral supervisory relationship, we are left with a sense of futility of how scholarly work preoccupied with social justice may confront, let alone transform, the larger sociopolitical realities with which we aim to engage. In the light of calls to turn precarity into a productive pedagogical space for ethical action – often regarded as a ‘pedagogy for precarity’, we draw from Blommaert’s (2005) sociolinguistic theory of voice to account for how we attempted to become recognisable to each other throughout the course of our supervisory meetings. In so doing, we reflect on the implications of our analysis for politically engaged academic research, while linking with wider language scholarship on the possibility for, and imaginability of, social transformation in higher education spaces.
The battle for whole-child approaches: Examining the motivations, strategies and successes of a parents’ resistance movement against a performance regime in a local Norwegian school system
Authors: Skedsmo Guri , Camphuijsen Marjolein K.
Published: September 2022

In different parts of the world, social movements led by parents, educators, and professional organizations have emerged that resist educational standardisation and use of (high stakes) standardised tests, and that push for educational change. With the aim of extending empirical coverage of protest movements in non-English speaking countries, this study examines a Norwegian parental movement called Foreldreopprør i Osloskolen (FiO) (in English: Parental Uprising in the Oslo School). We draw on exploratory research based on in-depth interviews with initiators and members of FiO (n=8), as well as documentary and press analysis and an examination of social media sites. The analysis sheds light on the emergence and nature of the movement, parents’ main motivations and discourses, as well as the movements’ collective action strategies and main successes. The findings highlight how rather than opting-out their children from standardised tests, some Norwegian parents chose to opt-out their children from public schools, while continuing their fight for whole-child approaches in public education.
Disputas em torno das avaliações na educação infantil no Brasil
Authors: Rodrigues Cibele Maria Lima , Almeida Karla Wanessa Carvalho de , Simões Patricia Maria Uchôa
Published: September 2022

O debate das avaliações padronizadas tornou-se uma agenda global imposta pelas organizações multilaterais. Essas provas, no contexto do neoliberal, impõem o empobrecimento do currículo e ainda o forte controle do trabalho docente. Por outro lado, também causa resistência por parte dos movimentos sociais. Este artigo aborda esse processo de disputas por hegemonia no contexto da Educação Infantil no Brasil (nos últimos anos). A pesquisa analisa o protagonismo do Movimento Inter-Fóruns de Educação Infantil no Brasil (MIEIB) no contexto dos governos do Partido dos Trabalhadores (Lula e Dilma). Nesse período, o movimento teve uma relação de agonismo com a gestão federal que permitiu discutir sobre diferentes formas de avaliar da qualidade, a partir do discurso do direito à educação. Atualmente, esse diálogo democrático se acabou e a relação entre o movimento e o governo se tornou antagonista. E a gestão de Bolsonaro deseja impor as avaliações para educação infantil.
Anti-standardization and testing opt-out movements in education: Resistance, disputes and transformation
Authors: Campos Martinez Javier , Falabella Alejandra , Holloway Jessica , Santori Diego
Published: September 2022

Testing, scoring comparison and accountability policies have become a ubiquitous part of schooling across most countries in the 21st century. The persistence of these hyper-surveillance measures has occurred in spite of an accumulative and increasing amount of evidence that illustrates negative effects of these kinds of policies. Meanwhile, diverse school actors have grown increasingly skeptical of how tests are being used, leading various groups to mobilize and resist such trends in education. This special issue looks at these resistance movements to school accountability measures across the world, gathering experiences of resistance from movements in countries in South America, Europe, North America, and Asia. This paper provides theoretical tools for analyzing resistance and presents an overview of these movements, highlighting common trends and variations referring to their goals, political strategies and outcomes.
HUBUNGAN ANTARA DUKUNGAN SOSIAL TEMAN SEBAYA DENGAN PENYESUAIAN DIRI SISWA PONDOK PESANTREN KELAS X MA RIBATUL MUTA’ALLIMIN PEKALONGAN
Authors: Zalika Ridya Dara Zalika , Rusmawati Diana
Published: September 2022

Penelitian ini bertujuan untuk mengetahui hubungan antara dukungan sosial teman sebaya dengan penyesuaian diri siswa Pondok Pesantren Kelas X MA Ribatul Muta’allimin Pekalongan. Populasi dalam penelitian ini adalah seluruh siswa Kelas X di MA Ribatul Muta’allimin Pekalongan. Sampel penelitian berjumlah 101 siswa dan menggunakan teknik cluster random sampling. Pengumpulan data dilakukan dengan menggunakan dua skala sebagai instrumen penelitian, yaitu skala penyesuaian diri (27 aitem dengan α = 0,898) dan skala dukungan sosial teman sebaya (35 aitem dengan α =  0,943). Berdasarkan hasil analisis data menggunakan Spearman’s Rho,  diketahui bahwa hipotesis yang diajukan dapat terbukti, yaitu terdapat hubungan positif antara dukungan sosial teman sebaya dengan penyesuaian diri siswa (rxy = 0,798 dengan p = 0,000). Yang berarti semakin tinggi dukungan sosial teman sebaya, maka akan semakin tinggi penyesuaian diri siswa; dan semakin rendah dukungan sosial teman sebaya maka semakin rendah pula penyesuaian diri siswa.
HUBUNGAN ANTARA DUKUNGAN SOSIAL DAN WORK ENGAGEMENT PADA GURU SEKOLAH LUAR BIASA DI KOTA CILEGON DAN SERANG
Authors: Saniya Adila Mutiara , Dewi Endah Kumala
Published: September 2022

Dukungan sosial adalah upaya yang dilakukan kepada individu yang bertujuan untuk upaya pemberian bantuan kepada seseorang untuk membantu menyelesaikan masalah tertentu, sedangkan work engagement adalah keterlibatan seorang karyawan pada pekerjaannya. Penelitian ini memiliki tujuan untuk mengetahui hubungan antara dukungan sosial dan work engagement pada guru Sekolah Luar Biasa di Kota Cilegon dan Serang. Populasi dalam penelitian ini adalah 203 guru Sekolah Luar Biasa dan sampel penelitian sebanyak 127 guru Sekolah Luar Biasa. Guru Sekolah Luar Biasa dipilih karena dengan berbagai tantangan pada pekerjaannya, mereka harus bertahan dan tetap memberikan yang terbaik bagi pekerjaannya. Teknik sampling yang digunakan adalah purposive sampling. Alat ukur yang digunakan adalah skala psikologi, yaitu skala dukungan sosial (31 item) dan skala work engagement (32 item). Berdasarkan metode analisis regresi sederhana didapatkan hasil koefisien determinasi (r2) = .049, F(6,374) = 3.84, dan signifikansi p = 0,013 (p<0,05) dengan kesimpulan bahwa adanya hubungan yang positif antara dukungan sosial dengan work engagement pada guru SLB di Kota Cilegon dan Serang. Dukungan sosial memberikan pengaruh sebesar 4,9% kepada work engagement.
HUBUNGAN ANTARA RESILIENSI DENGAN MOTIVASI BERPRESTASI PADA ATLET MAHASISWA JALUR PRESTASI DI SEMARANG
Authors: Fauziah Anisia , Widodo Prasetyo Budi
Published: September 2022

Setiap tahun universitas selalu membuka penerimaan mahasiswa baru. Salah satu jalur favorit siswa – siswi yang memiliki prestasi baik dalam bidang akademik maupun non akademik adalah jalur prestasi. Terdapat sepuluh universitas negeri maupun swasta di Semarang yang membuka jalur prestasi khususnya jalur prestasi olahraga. Atlet mahasiswa jalur prestasi dituntut mengoptimalkan keinginan untuk berprestasi, sehingga mampu mempertahankan prestasi non akademik dan tetap mampu mengikuti kegiatan akademik. Salah satu faktor yang berperan terhadap motivasi berprestasi adalah resiliensi. Penelitian ini bertujuan untuk melihat hubungan antara resiliensi dengan motivasi berprestasi atlet mahasiswa jalur prestasi di Semarang. Jumlah sampel pada penelitian ini 120 responden. Teknik sampling yang digunakan adalah cluster random sampling. Alat ukur yang digunakan yakni skala motivasi berprestasi (25 aitem valid, α = 0,870) dan skala resiliensi (31 aitem valid, α = 0,890). Hasil penelitian menunjukkan koefisien korelasi analisis regresi sederhana rxy = 0,454 dengan nilai signifikansi sebesar 0,000 (p<0,05). Hipotesis yang telah diajukan diterima dan terdapat hubungan positif yang signifikan antara resiliensi dengan motivasi berprestasi.
Pengaruh Kondisi Oseanografi Terhadap Pola Sebaran Sedimen Dasar di Perairan Mangunharjo, Kota Semarang
Authors: Purba Johanna R N D , Setiyono Heryoso , Atmodjo Warsito , Muslim Muslim , Widada Sugeng
Published: September 2022

Perairan Mangunharjo merupakan perairan yang terletak di Kota Semarang dan banyak dijadikan sebagai tempat pusat berbagai kegiatan vital seperti perumahan penduduk, pertambakan, rekreasi dan tak kalah penting sarana perhubungan. Dinamika pesisir yang terjadi di Perairan Mangunharjo yaitu sedimentasi dan abrasi menjadi permasalahan yang sering terjadi. Penanganan dinamika pantai memerlukan kajian terkait sedimen dasar perairan untuk memperoleh solusi yang tepat terhadap permasalahan. Target yang ingin diperoleh dari studi ini untuk mengetahui pengaruh dari faktor oseanografi seperti arus, gelombang, angin, pasut dan lainnya terhadap pola sebaran sedimen dasar. Data penelitian yang digunakan berupa data sedimen dasar sebagai data pengukuran lapangan, sementara untuk data arus laut, gelombang laut, angin, pasang surut, batimetri, debit sungai bersumber data sekunder. Pengolahan data sedimen dasar menggunakan metode granulometri, pengolahan data gelombang dan angin menggunakan metode SMB dan data pasang surut diolah dan dianalisa menggunakan metode admiralty. Hasil penelitian diperoleh bahwa Perairan Mangunharjo didominasi oleh ukuran butir pasir, lanau, dan lempung yang tersebar pada kedalaman 1 - 5 meter. Persentase sebaran sedimen dengan jenis lempung lebih besar di setiap stasiun. Faktor oseanografi yaitu arus, gelombang dan pasang surut memiliki pengaruh terhadap persebaran dan jenis sedimen. Arus adalah faktor oseanografi yang paling berpengaruh terhadap sebaran sedimen dasar perairan Mangunharjo.
Sebaran Sedimen Dasar Perairan dan Pola Arus Laut Di Muara Sungai Loji, Pekalongan
Authors: Pawitra Misbahul Diptya , Indrayanti Elis , Yusuf Muh , Zainuri Muhammad
Published: September 2022

Muara Sungai Loji merupakan salah satu muara sungai yang mempengaruhi sedimentasi Pantai Pekalongan, dimana muara ini digunakan sebagai jalur lalu lintas kapal. Hal tersebut dapat menimbulkan pendangkalan yang bisa mengurangi kedalaman laut. Penelitian ini bertujuan untuk mengetahui hubungan antara sedimen dasar dengan pola arus laut di Muara Sungai Loji, Pekalongan. Sampel sedimen dasar diambil menggunakan sedimen grab dan pengambilan data arus menggunakan ADCP (Acoustic Doppler Current Profiler). Pemodelan arus menggunakan pendekatan hidrodinamika 2D, dengan software MIKE21. Sementara itu sampel sedimen diolah menggunakan metode pengayakan dan pemipetan. Hasil dari penelitian ini menunjukan bahwa ukuran butir sedimen dasar yang ditemukan berupa pasir, lanau pasiran, dan lanau. Pola arus hasil pemodelan arus didapatkan kecepatan arus pada kondisi pasang yaitu 0,096 m/s, surut menuju pasang sebesar 0,18 m/s, surut terendah sebesar 0,13 m/s, dan pasang menuju surut sebesar 0,15 m/s. Arus laut mempengaruhi distribusi sedimen dasar dimana kecepatan arus yang lebih kecil akan mengendapkan sedimen dengan ukuran halus seperti di daerah sebelah timur muara, sedangkan di sebelah barat muara terdapat arus lebih tinggi sehingga mengendapkan sedimen lebih kasar karena ukuran sedimen yang halus mengalami tersuspensi. Kata kunci: Pola Arus, Sedimen Dasar, Ukuran Butir Sedimen, Muara Sungai Loji Abstract The estuary of the Loji River is one of the river estuaries that affects the sedimentation of Pekalongan Beach, where this estuary is used as a ship traffic lane. This can cause siltation which can reduce the depth of the sea. This study aims to determine the relationship between bottom sediment and ocean currents at the Loji River Estuary, Pekalongan. The bottom sediment samples were taken using a sediment grab and the current data collection using the ADCP (Acoustic Doppler Current Profiler). The current modeling uses a 2D hydrodynamic approach, using MIKE21 software. Meanwhile, sediment samples were processed using sieving and pipetting methods. The results of this study indicate that the grain size of the basic sediment found in the form of sand, sandy silt, and silt. The current pattern of the current modeling results obtained that the current velocity at high tide is 0.096 m/s, low tide is 0.18 m/s, lowest ebb is 0.13 m/s, and high tide is 0.15 m/s. Ocean currents affect the distribution of bottom sediments where a smaller current velocity will deposit fine-sized sediments such as in the east of the estuary, while in the west of the estuary there are higher currents so that coarser sediments are deposited because the finer sediments are suspended. Keywords: Current Pattern, Base Sediment, Sediment Grain Size, Loji River Estuary
Studi Koefisien Distribusi 137Cs di Perairan Muara Cisadane, Teluk Jakarta
Authors: Latifah Lilik , Suseno Heny , Muslim Muslim , Yahya Mohamad Nur
Published: September 2022

Radiocesium berada di lingkungan laut berasal dari jatuhan atmosferik (global fallout) akibat dari kecelakaan PLTN di Chernobyl dan Fukushima pada tahun 1986 dan 2011 lalu. Radionuklida 137Cs memiliki sifat konservatif sehingga dapat menyebar hingga ke perairan global. Tujuan penelitian ini adalah untuk mengetahui tingkat aktivitas dan sebaran 137Cs pada air laut dan sedimen, serta mengetahui nilai koefisien distribusi 137Cs. Sampel air dan sedimen dikumpulkan dari perairan Muara Cisadane, Teluk Jakarta. Aktivitas 137Cs diukur menggunakan Spektrometer Gamma HPGe Canberra 3000 di laboratorium Badan Tenaga Nuklir Nasional dengan aktivitas 137Cs pada sampel air yaitu berkisar antara 0.30-1.12 Bq.l-1, dan 137Cs pada sampel sedimen berkisar antara 0.60-1.22 Bq.kg-1. Konsentrasi koefisien distribusi 137Cs yang berkisar antara 0.65-3.40 L.kg-1 dengan rata-rata 1.401 L.kg-1.