bluuebunny/crossref_metadata_2025 huggingface dataset:
Desafios da atuação fisioterapêutica com idosos na atenção domiciliar pela estratégia de saúde da família no SUS: Revisão integrativa
Authors: Santos Sissiane Viana dos , Ferro Thauan Narciso de Lima , Alves Ahyas Sydcley Santos
Published: January 2024

Este estudo objetivou investigar os desafios da atuação fisioterapêutica com idosos na Atenção Domiciliar pela Estratégia de Saúde da Família no SUS, através de revisão integrativa de literatura. As fontes de dados utilizadas foram bases eletrônicas e busca na lista de referência dos artigos. Os artigos foram buscados nas  bases de dados eletrônicas: BVS, Scielo, PubMed, Lilacs e PEDro, utilizando as estratégias busca de combinações dos descritores a seguir selecionados da base Descritores em Ciências da Saúde (DECS): Fisioterapia, Idoso, Assistência domiciliar, Saúde da família e Sistema único de saúde. Objetivou-se, na pesquisa dos artigos, incluir publicações científicas que abordavam o objetivo da pesquisa, que estivesse com texto completo e disponível, contemplando pelo menos um dos descritores escolhidos, publicados de 2018 a 2023, em português, inglês e/ou espanhol. Após aplicação dos critérios e leitura dos achados foram selecionados 8 artigos para compor a revisão. Observou-se que a pode a atuação da fisioterapia domiciliar para idosos na Estratégia de Saúde da Família (ESF) pode ser de grande importância, apresentando uma gama de possibilidades de condutas, protocolos e parâmetros. Constatou-se que neste contexto enfrenta-se uma série de desafios complexos. Para avançar no entendimento destes desafios, são necessárias pesquisas que integrem metodologias variadas, como estudos longitudinais que acompanhem o impacto das intervenções ao longo do tempo, análises qualitativas para compreender as perspectivas dos idosos, cuidadores e profissionais de saúde envolvidos, e estudos de caso que identifiquem boas práticas e desafios enfrentados em contextos específicos.
Papillomavirus humano e coinfecções por Chlamydia trachomatis e Gardnerella vaginalis em mulheres atendidas em um laboratório particular na cidade de Aracaju-Sergipe, Brasil
Authors: Santos Rafaela Windy Farias dos , Santana Odara Regina Menezes de , Santana Danielly Silva de , Lóz Sinthia Regina dos Santos , Melo Juçara Santos de , Santos Pâmela Silva , Santos Renata Cibelle Farias dos
Published: January 2024

O papillomavirus humano (HPV) está associado ao desenvolvimento do câncer de colo uterino, em que diferentes fatores podem estar associados com a persistência, potencialização e a progressão de lesões pré-cancerosas, um destes fatores são as coinfecções entre HPV, Chlamydia trachomatis (CT) e Gardnerella vaginalis (GV). Assim, o objetivo do trabalho é verificar a incidência do HPV e as coinfecções por CT e GV em mulheres atendidas em um laboratório particular de Aracaju, localizada no estado de Sergipe. O estudo é do tipo descritivo, transversal e retrospectivo, sendo analisados os resultados dos exames para a genotipagem de HPV alto risco, pesquisa de CT e o exame citopatológico, que foram realizados no período entre janeiro à outubro de 2022. No período de estudo, 1.312 mulheres realizaram a coleta de secreção vaginal, sendo detectado outros genótipos de alto risco oncogênico do HPV que não 16 e/ou 18 em 80,65% (296/367) dos casos, seguido do HPV-16 com 8,71% (32/367) e HPV-18 com 3,82% (14/367). Dentre as pacientes infectadas por HPV, houve 30,7% (113/367) casos de coinfecções com 14,16% (16/113) por CT e 81,42% (92/113) por GV, além dos achados de tripla infecção, em 4,42% (5/113) dos casos. Assim, foi observado que houve uma maior incidência de outros genótipos do HPV que não 16 e/ou 18, além de ser encontrado casos de coinfecções duplas e triplas nas mulheres atendidas no laboratório.
Theories of education and educational research: Contributions to the continuous education of teachers
Authors: Silva Francisco Marcones Moura , Sena Edilene Ferreira de , Barroso Ana Carla Araujo , Furtado Luís Távora Ribeiro
Published: January 2024

Scientific research in the educational field can become a great ally with regard to the construction of new knowledge and critical training for education professionals. It is through research that we will seek to become aware that we are producers of transforming knowledge, facilitating improvement and encouraging pedagogical work. Based on the above, this article aims to address the theories of education in some of its phases and its influence on the preparation of the educator, reflecting on the formation of teachers from the educational research which seeks to follow the course, maintaining a relationship dialectic between theory and practice, that is, from “understanding” to pedagogical “doing”. For the construction of the theoretical foundation of this research, we selected some authors, among them: Batista, Saviani, Suchodolski, Freire and Schnetzler. Discussions point out that teaching as a transforming action in society is renewed from teacher training, which is the result of research based on theories of education.
SOSYAL MEDYA ARACILI KURUMSAL SOSYAL SORUMLULUK İLETİŞİMİNİN DEPREM AFETİ BAĞLAMINDA ANALİZİ: FORTUNE 500 TÜRKİYE LİSTESİNDE YER ALAN KURUMLARIN FAALİYETLERİ ÜZERİNE BİR İNCELEME
Authors: BOZTEPE TAŞKIRAN Hatun
Published: January 2024

Halkla ilişkilerin temel bir uygulama alanı olan kurumsal sosyal sorumluluk, kurumlar ile hedef kitleler arasında toplumsal ilişkilerin geliştirilmesi amacına odaklanmaktadır. Toplumun tümünü ya da büyük bir kısmını fiziksel, ekonomik ve sosyal açılardan etkileyen, hayatın olağan akışını değiştiren ve etkilenen kişi ve grupların kendi imkân ve kaynakları ile üstesinden gelmesinin mümkün olmadığı afetler ise kurumların, toplumun birer üyesi olarak afete yönelik yanıt vermesinin beklendiği dönemlerdir. Sosyal sorumluluk iletişimi açısından literatür değerlendirildiğinde sosyal medyanın sosyal sorumluluk iletişimi faaliyetleri için önemli bir kapasite sunduğu da görülmektedir. Bu bilgiler doğrultusunda bu çalışma, sosyal medya aracılı kurumsal sosyal sorumluluk iletişimini deprem afeti bağlamında incelemek üzere planlanmış ve Türkiye’nin en büyük 50 kurumunun 6 Şubat 2023 tarihinde Kahramanmaraş merkezli depremlere yönelik sosyal medya aracılı kurumsal sosyal sorumluluk iletişimi nitel araştırma yöntemlerinden içerik analizi tekniği ile incelenmiştir. Elde edilen bulgular kurumların depremin etkilerine yönelik hızlı yanıtlar vermeye odaklanan barınma ve konaklama, yiyecek ve giyecek desteği gibi sosyal sorumluluk faaliyetlerine odaklandıklarını göstermiş ve ayrıca depremin, zaman ilerledikçe sosyal medya aracılı sosyal sorumluluk iletişimi açısından kurumsal bir gündem maddesi olmaktan uzaklaştığına işaret etmiştir.
Pendeteksi Persentase Kadar Alkohol Dengan Kontrol PID Berbasis IOT
Authors: Ayu Handira Putri , Azis Pangestu M , Sulistyo Eko , Silalahi Parulian
Published: January 2024

In order to find out the alcohol content in drinks circulating in the community, measurements and tests are usually carried out in the laboratory. This takes quite a long time. To solve this problem, we need a tool that can measure alcohol quickly and accurately. This research aims to design and manufacture a detector for the percentage of alcohol content, especially in drinks, using an IoT (Internet of Thing)-based MQ3 sensor as a monitoring system that is connected directly to a smartphone. The method used in this research is PID (Proportional Integral Derivative). By setting the PID tuning with a value of kp=4, ki=3, kd=0.0001 and a setpoint of 210 is used to produce an optimal reading of the alcohol content in the drink. Readings using PID control have an average error value of 1.26%, otherwise without using PID control, the average error value is 8.12%. In order to see the alcohol content of the experimental results of the type of drink detected, it will be displayed on the blynk application on the smartphone.
Pengaruh Variasi Waktu Operasi Mesin Terhadap Perubahan Nilai Viskositas Dan Total Acid Number Untuk Pelumas Gearbox
Authors: Putra Dika Septayama , Ariyanto Ariyanto , Feriadi Indra
Published: January 2024

Lubricating is one of the methods to prevent temperature rise and wear due to friction among gearbox components. The lubricant quality is an essential factor in ensuring lubricant functions optimally. Long-term use of the machine changes lubricant quality, which can be measured by viscosity parameters and Total Acid Number (TAN). This research aims to determine the effect of machine operating time on viscosity and TAN lubricant changes of milling machine gearbox heads (Headstock) used for educational purposes. The study used a quantitative research method in which data was collected using statistical data analysis tools and selecting a random sampling. Forty samples of Shell Tellus 46 lubricant were taken every 5 hours for 200 operation hours with an operating temperature of 27.5-44.4°C and 300 rpm speed. Viscosity testing uses Visgage and Fluidscan to check TAN. Data analysis using a simple linear regression analysis method with the statistical software Minitab 21.3. The results showed that operating time had a significant effect on: 1) a 95% change in viscosity value with 3.67 cSt (7.39%) decline or 0.018776 cSt per operation hour; 2) 99.34% change in TAN value with 0.27 mg KOH/g (16.56%) increase or 0.001297 mg KOH/g per operation hour.
Rancang Bangun Sistem Informasi Inventaris Barang Pada SMKN 1 Parittiga
Authors: Sundara Ayu , Andriyanto Sidhiq , Pratama M. Setya
Published: January 2024

This research aims to design and build a web-based goods inventory system at SMKN 1 Parittiga to assist the goods inventory section in submitting goods, information on incoming and outgoing goods and the condition of the goods. The research method used is a descriptive qualitative method and the data collection method is the method of observation, interviews, literature and documentation and uses the waterfall method as a software development method. This web-based inventory information system at SMKN 1 Parittiga was built using sublime text PHP, Bootstrap, CI, database, and MySQL programming languages. The goods inventory information system at SMKN 1 Parittiga makes it easy for admins to submit goods, information on incoming and outgoing goods, and information on the condition of goods. For this reason, in this project, analysis and design of the system have been carried out, with the final result that this inventory information system has proven to facilitate and greatly assist the performance of the inventory section.
Pengaruh Salinitas Pasir Terhadap Kuat Tekan Bata
Authors: Mascik Deny perayuda , S Suzen Zaldy , Ary W Ilham
Published: January 2024

The development of technology, especially in the construction field, in manufacturing bricks is increasingly rapid and varied, one of which is lightweight brick. The light brick is a composite building material made of sand, cement, limestone, gypsum, water, and aluminum powder. In general, lightweight bricks have two types, Autoclaved Aerated Concrete (AAC) and Cellular Lightweight Concrete (CLC). Light bricks were created to lighten the structural load of a construction building, speed up construction, and minimize material waste during the wall installation process. Sand is a staple in the construction process. In addition, sand material cannot be separated from its use in the industrial world. The Taguchi method is a methodology in engineering that aims to improve the quality of products and processes and can reduce costs and resources to a minimum. The compressive strength of the concrete load is the magnitude of the load per unit area, which causes the concrete specimen to collapse when loaded with a specific compressive force, which is generated by the press machine; the compressive strength of the concrete will increase with increasing age of the concrete, where the compressive strength of the concrete will increase rapidly (linearly). Salinity is the salt content dissolved in water which is one of the physical parameters that can affect the quality of water, soil and sand.
Pengaruh Parameter Proses 3D Printing Terhadap Kuat Bentur Menggunakan Fiamen Polycarbonate
Authors: Rivaldi Muhammad , Yunus Muhammad , Pristiansyah Pristiansyah
Published: January 2024

The existence of this 3D printing technology in manufacturing has brought big companies to the world of industry. One of the rapid prototyping technologies that are often found in the market today is the FDM type of technology. Fused Deposition Modeling (FDM) technology is a printing technique used to print products using fibrous materials, printed products have ideal geometric characteristics. Impact test is a method used to determine the strength, hardness and ductility of a material. In this impact test, rapid loading is used. The method used is Taguchi and this study aims to obtain optimal process parameters in the impact test. Printing was carried out with a 3D printing machine Creality Ender 3 which printed 81 specimens. Then the impact test was carried out so that the highest average value of the impact test results was in experiment number 27 with a value of 0.00392 joules and for the lowest impact test value was in experiment number 1 with a value of 0.00291 Joules. The method used in this process parameter optimization research is the Taguchi L9 OA method. for the factors used, namely printing speed, infill layer thickness, wall thickness, print temperature. . the results of the research that has been carried out show the optimum parameter values ​​and parameters that influence the impact test successively, namely printing speed (40mm/s), infill layer thickness (0.2mm), wall thickness (2.0mm), print temperature (250 ℃), bed temperature(250℃), infill overlap(30mm), raft extra margin(7.5mm), wall printing speed(22.5mm/s). It was found that the highest average value of the impact test results was in experiment number 2 with a value of 0.031 joules and the lowest impact test value was in experiment number 7 with a value of 0.026 Joules.
Pemfokusan Solar Water Heater Menggunakan Reflektor Parabolik
Authors: Saputro Ego Wisnu , Ocsirendi Ocsirendi , Saputra Zanu
Published: January 2024

The need for hot water is very important for the health of every human being. Not only from health, industrial fields such as hospitality have a high level of use of warm water. High usage must be balanced with existing income. However, in the case of heating water on a large scale, a large source is also needed. To overcome this, a solar water heating system was created in order to overcome this problem while at the same time utilizing natural heat sources from the sun. Solar water heaters with parabolic reflectors are made to be able to heat water by focusing light by the parabolic reflectors onto the collector pipe. In optimizing it, a tracking system for the sun is also added so that the heat absorption that occurs can be more effective. Tracking is carried out for 6 hours, starting from 09.00 to 15.00. It can be seen that the highest temperature increase uses the 10 minutes delay method of 55.18°C and the highest temperature for heating uses the continuous method of 44.62°C with the same heating time from 13.30-13.40. Comparison of the heat absorption efficiency of the collector pipe is better by using the 10 minutes delay method of 35.04% in terms of achieving the highest temperature. As for the comparison of the whole system, the use of the 10 minutes delay method has a higher efficiency of 30.95% compared to the continuous heating method.
Optimasi Parameter Produk 3D Printing Terhadap Kuat Tarik Menggunakan Filamen TPU (Thermoplastic Polyurethane)
Authors: Redy Redy , Hasdiansah Hasdiansah , Sirwansyah Suzen Zaldy
Published: January 2024

3D Printing is a change from printing technology which is being able to produce and design sophisticated structures in one unity. 3D printing is a process fabrication Fused Deposition Modeling (FDM) , that is Additive Manufacturing (AM) technology, which is a system that works by adding material layer by layer to form 3D objects. This research will use TPU (Thermoplastic polyurethane) filaments. Mechanically this TPU filament has good elastic mechanical properties. This research aims to obtain the highest tensile test value from the results of 3D printer printing with the specifications to be used, namely layer thickness, infill pattern, and Z-orientation. Printing will be carried out using the Anet A8 Plus machine and for the type of specimen using the ASTM D-638 type V standard. This study used taguchi L9 with 3 factors and 3 levels which printed 27 specimens. So that the highest score obtained in the tensile test with experiment number 7 was 12.97MPa and the lowest tensile test value was obtained in experiment number 2 with a value of 11.87MPa. For the results of the calculation of the S/N ratio, the most influential factor is the Z-orientation.
Optimalisasi Kinerja Archimedes Screw Menggunakan Sistem Transmisi Terhadap Output Daya (Watt)
Authors: Fikri Fikri , Ramdhani Dedy , Hasdiansah Hasdiansah
Published: January 2024

Water energy sources at low head and discharge can be utilized as a generator of electrical energy, one of which is by using the Archimedes screw turbine, the Screw turbine is used in Pico Hydro Power Plants (PLPTH) as a small-scale power plant by utilizing water energy from river flow. The purpose of this research is to optimize the performance of the Archimedes screw turbine Pico Hydro Power Plant so as to produce a better power output. In this study the authors designed an Archimedes screw turbine model with a screw angle of 28°, 5 blade type screw, and 40° turbine tilt. This study focuses on a transmission system using a belt and pulley ratio of 1:3, with two levels of transmission, and a turbine tilt angle of 40°. The method used in this study is the experimental method. As a result, after trials were carried out on a stream located in the Sungailiat Archipelago Fishing Port area, the highest turbine rotation value was 146 rpm and generator rotation 756 rpm, resulting in a power output of 8 watts within 45 minutes of the trial period in the field. This means that after modifying the Archimedes screw turbine with the transmission system and the turbine tilt angle of 40°, the resulting power output increases.
Fenomena Las Gesek (Friction Welding) Dengan Variasi Waktu Gesek Pada Material AISI 1040 Dengan Kuningan
Authors: Sofwan Ibnu , Sugiyarto  , Erwanto Erwanto
Published: January 2024

Friction welding is one of the metal joining methods, by utilizing the heat generated from the physical contact of two metals in the form of friction that occurs continuously on the two metals to produce a welding joint.  This research aims to find out what phenomena occur in the connection between AISI 1040 and Brass. The materials used are AISI 1040 solid cylinder with a diameter of 16 mm and brass solid cylinder with a diameter of 16 mm. This research uses experimental method. The friction time used is 6 minutes, 7 minutes, and 8 minutes with a variation of pressing distance of 3 mm, 4 mm, 5 mm with a rotating speed of 1000 rpm. The free heat process of 200 ℃ was carried out on AISI 1040 before the welding process. From the time variation and pressing distance variation that has been carried out in this study, the results obtained from the two materials do not occur. There are several phenomena seen after the welding process such as the occurrence of plastic deformation in brass and the formation of a brass thermoplastic layer attached to the surface of AISI 1040, in the time variation of 6 minutes and 7 minutes the brass thermoplastic attached to AISI 1040 is small and thin, while in the time variation of 8 minutes the brass thermoplastic layer attached to AISI is quite a lot and thick but no connection has occurred. This friction welding method is likely to connect the AISI 1040 material with brass by increasing the friction time performed.
Implementasi Proses Manufaktur Pembangkit Listrik Bertenaga Angin Dan Surya Di Pesisir Pantai Matras
Authors: Kevin Cristian , Rollastin Boy , Husman Husman
Published: January 2024

Indonesia is a country rich in natural resources and natural resources. These natural resources can be in the form of wind and solar heat, wind and solar heat can be utilized as an alternative to power generation with the type of power generator in the form of wind turbines and solar panels. In certain areas there are still many that require a supply of electric power, for example in the Turun Aban Beach area. At that location there is no electricity supply at all. Meanwhile, the fishermen at the Turun Aban Beach location often carry out the process of transporting their catch at night. Therefore, to meet the needs of these fishermen, they can use alternative power generators in the form of wind turbines and solar panels. Coastal areas have great potential for wind speed and solar heat. This research examines the process of implementing wind and solar power generators that will be used as alternative lighting for fishermen on the Turun Aban Beach, Matras Bangka Village. The process of making the tool will be carried out at the PolmanBabel Mechanical Workshop. Making this tool will go through several stages such as cutting the frame material, welding, turning and also assembling the electrical circuit on the tool.
Taming Digital Influence: Hypernudging and the Role for European Competition Law
Authors:   , Morozovaitė Viktorija
Published: January 2024

This dissertation examines hypernudging – a sophisticated form of user influencing online - within the context of big technology companies and their prominent role in shaping digital markets. Digital platforms are increasingly adopting user influencing practices to encourage engagement and spending, sometimes to the detriment of the users. This research takes the form of a legal and normative assessment of the role that European competition law should play in addressing the challenges posed by hypernudging by big technology companies.

The thesis unfolds through three interrelated themes. Firstly, it defines hypernudging in its multiple aspects by establishing a consolidated framework and situating these practices within the transdisciplinary debate on user influencing, underscoring the risks of individual and collective harm. Secondly, the study examines hypernudging in the context of the abuse of dominance prohibition under European competition law. Using case studies in the ad tech and voice assistant markets, it shows that hypernudging can facilitate exclusionary self-preferencing behavior by powerful firms. Furthermore, a case study on political microtargeting takes a normative perspective to question the boundaries of European competition law. It underlines the need for balancing between efficiency and democratic considerations, particularly as big technology companies extend their influence into the political sphere.

Finally, hypernudging is situated within the EU's digital policy agenda, highlighting the complexities policymakers face in regulating digital markets. While existing competition law theories can be applied to hypernudging, a normative stance advocates for an expanded role that facilitates the co-creation of digital markets in line with EU values.
HEALTH LAW IMPLEMENTATION REALIZES GENDER EQUALITY AND WOMEN'S EMPOWERMENT
Authors: Siregar Rospita , Hendri Jayadi
Published: January 2024

The millennium development program (MDGs) in quality health services, including women's reproductive health, is not evenly distributed, due to poor infrastructure and the unequal quality of health services between provinces. It is hoped that Indonesian Health has succeeded in achieving most of Indonesia's MDGs targets, namely 49 of the 67 MDGs indicators can be improved. In normative juridical research, law is conceptualized as norms or rules that apply in society. Using a statutory approach and a conceptual approach in health law. There is a guarantee of a sense of security in accordance with Article 28G Paragraph (1) of the 1945 Constitution, thereby providing women's rights in accordance with the mandate given by the state and guaranteeing rights without discrimination and gender differences. There is the Convention on the Elimination of All Forms of Discrimination against Women, which emphasizes that women's human rights are respected, then women's reproductive health rights have also been formulated by the International Planned Parenthood Federation (IPPF) since 1996, namely the right to equality and freedom from all forms of discrimination. In the end, support for women's empowerment must involve many parties from politics, PUG institutions, Human Resources, Budget, Gender Analysis Tools, and Community Participation.

Keywords: gender equality, health law, reproductive health rights
12 Eylül’ün İletişimsel Belleği ve Bir Bellek Taşıyıcısı Olarak Ahmet Kaya
Authors: Orhon Göze
Published: January 2024

Bu çalışma 12 Eylül’ün toplumsal belleğine özgül bir bakış açısıyla yaklaşmayı ve darbenin toplumsal belleğinin kuşaklararası niteliğini Ahmet Kaya örneği dolayımıyla çözümlemenin konusu haline getirmeyi hedeflemektedir. Kimi özgül nitelikleri nedeniyle Ahmet Kaya 12 Eylül belleğinin kuşak içi inşasında ve kuşaklar arası aktarımında önemli bir figür olarak ele alınmaktadır. 12 Eylül darbesini kişisel olarak deneyimlememiş, kendini sol siyasete referansla farklı biçimlerde tanımlayan, 1970-1999 arasında doğmuş kişilerle yapılan bir saha araştırmasının verilerine dayanan bu çalışmanın, görüşmecilerin bellek anlatıları aracılığıyla Ahmet Kaya’ya odaklanıyor olmasının nedeni, Kaya’nın hem kuşaklar arası bellek aktarımında hem de kuşak içi etkileşimlerde özgül bir rol oynamasıdır. Ahmet Kaya’nın bu özgül niteliği, onu hem 12 Eylül’ün iletişimsel belleğinin bir bileşeni, hem de bir bellek taşıyıcı olarak tartışılmasını olanaklı kılmaktadır. Çalışma ilkin 12 Eylül’ü deneyimleyen ve deneyimlemeyen grupları, köklerini Mannheim’ın kuşak kavrayışından alan bir kuşak tartışmasıyla ele almakta, daha sonra görüşmecilerin anlatılarının sunduğu veriyi kavramsal olarak iletişimsel bellek ve bellek taşıyıcısı tartışmalarını odağa alarak çözümlemektedir.
Antiimperialismo y panamericanismo: la Quinta Conferencia Panamericana (1923) y la Sexta Conferencia Panamericana (1928)
Authors: Rodríguez Campesino Aida
Published: January 2024

Las relaciones interamericanas se han producido históricamente en un marco de desigualdad. A comienzos del siglo XX, las amenazas imperialistas por parte de Estados Unidos hacia América Latina se convirtieron en una constante. En este texto se explora la resistencia latinoamericana a la injerencia estadounidense mediante el estudio de dos conferencias panamericanas: la de Santiago de Chile en 1923 y la de La Habana en 1928, enmarcadas en una década en la que el intervencionismo del país del norte alcanzó se hizo muy habitual. El antiimperialismo estuvo muy relacionado con el antiamericanismo y con una reinterpretación del movimiento panamericano.
Why do lactic acid bacteria thrive in chain elongation microbiomes?
Authors: Ulčar Barbara , Regueira Alberte , Podojsteršek Maja , Boon Nico , Ganigué Ramon
Published: January 2024

Efficient waste management is necessary to transition towards a more sustainable society. An emerging trend is to use mixed culture biotechnology to produce chemicals from organic waste. Insights into the metabolic interactions between community members and their growth characterization are needed to mediate knowledge-driven bioprocess development and optimization. Here, a granular sludge bioprocess for the production of caproic acid through sugar-based chain elongation metabolism was established. Lactic acid and chain-elongating bacteria were identified as the two main functional guilds in the granular community. The growth features of the main community representatives (isolate Limosilactobacillus musocae G03 for lactic acid bacteria and type strain Caproiciproducens lactatifermentans for chain-elongating bacteria) were characterized. The measured growth rates of lactic acid bacteria (0.051 ± 0.005 h−1) were two times higher than those of chain-elongating bacteria (0.026 ± 0.004 h−1), while the biomass yields of lactic acid bacteria (0.120 ± 0.005 g biomass/g glucose) were two times lower than that of chain-elongating bacteria (0.239 ± 0.007 g biomass/g glucose). This points towards differential growth strategies, with lactic acid bacteria resembling that of a r-strategist and chain-elongating bacteria resembling that of a K-strategist. Furthermore, the half-saturation constant of glucose for L. mucosae was determined to be 0.35 ± 0.05 g/L of glucose. A linear trend of caproic acid inhibition on the growth of L. mucosae was observed, and the growth inhibitory caproic acid concentration was predicted to be 13.6 ± 0.5 g/L, which is the highest reported so far. The pre-adjustment of L. mucosae to 4 g/L of caproic acid did not improve the overall resistance to it, but did restore the growth rates at low caproic acid concentrations (1–4 g/L) to the baseline values (i.e., growth rate at 0 g/L of caproic acid). High resistance to caproic acid enables lactic acid bacteria to persist and thrive in the systems intended for caproic acid production. Here, insights into the growth of two main functional guilds of sugar-based chain elongation systems are provided which allows for a better understanding of their interactions and promotes future bioprocess design and optimization.
The collective action dilemma and its solution in the governance of urban community public space—an empirical analysis from Tianjin
Authors: Li Yapeng
Published: January 2024

The governance of community public space is one of the most concerned issue of people’s livelihood, and its effectiveness determines the residents’ living experience and even the stability of the grassroots political authority. In order to explore the factors of the effectiveness of community public space governance and the path to enhance the effectiveness of community public space governance, this analysis combines the influencing factors with the three stages of identification, management and confirmation in public value management theory, and constructs an indicator system for measuring and analyzing the effectiveness of community public space governance. The fsQCA3.0 is used to analyze the data of 300 residents in Tianjin, China, after which several aims that affect the effectiveness of community public space governance are obtained. The results show that the paths of “resident trust, property management is authoritative” and “customer oriented, democratic consultation” that bring higher governance efficiency have a consistency of 0.874761 and a coverage of 0.480994, which explains well what combination of antecedent factors should be present in communities with high public space governance effectiveness. Based on this, in order to improve the governance efficiency of community public space, improving residents’ living experience and promote the modernization of China’s urban construction, the government, property management company, owners’ committee and neighborhood committee should increase investment in enhancing policy support, establishing service concepts, introducing technology and equipment, enhancing residents’ trust and establishing dispute coordination agencies.
Characterization of Klebsiella pneumoniae carrying the blaNDM-1 gene in IncX3 plasmids and the rare In1765 in an IncFIB-IncHI1B plasmid
Authors: Ma Liman , Qu Ying , Wang Wenji , Wang Dongguo
Published: January 2024

Background

Today, the blaNDM gene is widely distributed on several plasmids from a variety of Gram-negative bacteria, primarily in transposons and gene cassettes within their multidrug-resistant (MDR) regions. This has led to the global dissemination of the blaNDM gene.

Methods

The determination of class A beta-lactamase, class B and D carbapenemases was performed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). Antimicrobial susceptibility testing was performed using both the BioMerieux VITEK2 system and antibiotic paper diffusion methods. Plasmid transfer was then evaluated by conjugation experiments and plasmid electroporation assays. To comprehensively analyze the complete genome of K. pneumoniae strain F11 and to investigate the presence of mobile genetic elements associated with antibiotic resistance and virulence genes, Nanopore and Illumina sequencing platforms were used, and bioinformatics methods were applied to analyze the obtained data.

Results

Our findings revealed that K. pneumoniae strain F11 carried class A beta-lactamase and classes B+D carbapenemases, and exhibited resistance to commonly used antibiotics, particularly tigecycline and ceftazidime/avibactam, due to the presence of relevant resistance genes. Plasmid transfer assays demonstrated successful recovery of plasmids pA_F11 and pB_F11, with average conjugation frequencies of 2.91×10-4 and 1.56×10-4, respectively. However, plasmids pC_F11 and pD_F11 failed in both conjugation and electroporation experiments. The MDR region of plasmid pA_F11 contained rare In1765, TnAs2, and TnAs3 elements. The MDR2 region of plasmid pB_F11 functioned as a mobile genetic “island” and lacked the blaNDM-1 gene, serving as a “bridge” connecting the early composite structure of bleMBL and blaNDM-1 to the recent composite structure. Additionally, the MDR1 region of plasmid pB_F11 comprised In27, TnAs1, TnAs3, and Tn2; and plasmid pC_F11 harbored the recent composite structure of bleMBL and blaNDM-1 within Tn3000 which partially contained partial Tn125.

Conclusion

This study demonstrated that complex combinations of transposons and integron overlaps, along with the synergistic effects of different drug resistance and virulence genes, led to a lack of effective therapeutic agents for strain F11, therefore its dissemination and prevalence should be strictly controlled.
Endogenous retroviruses Suppressyn and Syncytin-2 as innovative prognostic biomarkers in Acute Myeloid Leukemia
Authors: Shen Jiaxin , Wen Xiaofen , Xing Xueyang , Fozza Claudio , Sechi Leonardo Antonio
Published: January 2024

Introduction

Emerging evidence has proven that human endogenous retroviruses (HERVs) play a critical role in the pathogenesis of Acute Myeloid Leukemia (AML), whereas the specific HERVs influencing the prognosis of AML patients have yet to be fully understood.

Methods

In this study, a systematic exploration was achieved to identify potential prognostic HERVs for AML, sourced from TCGA and GTEx database. Differential analysis and functional enrichment studies were conducted using GO, KEGG, GSEA, and GSVA. The ESTIMATE algorithm was applied to explore the immune infiltration of HERVs in AML. A prognostic risk-score model was evaluated with predicted yearly accuracy using ROC analysis.

Results

Two HERVs Suppressyn and Syncytin-2, were identified as promising prognostic biomarkers, with high discrimination ability based on ROC analysis between AML and healthy cohorts from TCGA. Their expression was notably higher in AML patients compared to those in healthy individuals but correlates with favorable clinical outcomes in sub-groups such as white race, lower WBC counts, favorable and intermediate risks, and NPM1 or IDH1 mutation. Suppressyn and Syncytin-2 participated in immune-related pathways and exhibited correlations with multiple immune infiltration cells, such as T cells, mast cells, and tumor-associated macrophages. Finally, we developed a prognostic risk-scoring model combining Suppressyn and Syncytin-2, where a high risk-score is associated with better prognosis.

Discussion

Collectively, our findings revealed that Suppressyn and Syncytin-2 may act as valuable diagnostic and prognostic biomarkers for individuals with AML, while highlighting links between HERV activation, immunogenicity, and future therapeutic targets.
Causal inference of diachronic semantic maps from cross-linguistic synchronic polysemy data
Authors: Dellert Johannes
Published: January 2024

Semantic maps are used in lexical typology to summarize cross-linguistic implicational universals of co-expression between meanings in a domain. They are defined as networks which, using as few links as possible, connect the meanings so that every isolectic set (i.e., set of meanings that can be expressed by the same word in some language) forms a connected component. Due to the close connection between synchronic polysemies and semantic change, semantic maps are often interpreted diachronically as encoding potential pathways of semantic extension. While semantic maps are traditionally generated by hand, there have been attempts to automate this complex and non-deterministic process. I explore the problem from a new algorithmic angle by casting it in the framework of causal discovery, a field which explores the possibility of automatically inferring causal structures from observational data. I show that a standard causal inference algorithm can be used to reduce cross-linguistic polysemy data into minimal network structures which explain the observed polysemies. If the algorithm makes its link deletion decisions on the basis of the connected component criterion, the skeleton of the resulting causal structure is a synchronic semantic map. The arrows which are added to some links in the second stage can be interpreted as expressing the main tendencies of semantic extension. Much of the existing literature on semantic maps implicitly assumes that the data from the languages under analysis is correct and complete, whereas in reality, semantic map research is riddled by data quality and sparseness problems. To quantify the uncertainty inherent in the inferred diachronic semantic maps, I rely on bootstrapping on the language level to model the uncertainty caused by the given language sample, as well as on random link processing orders to explore the space of possible semantic maps for a given input. The maps inferred from the samples are then summarized into a consensus network where every link and arrow receives a confidence value. In experiments on cross-linguistic polysemy data of varying shapes, the resulting confidence values are found to mostly agree with previously published results, though challenges in directionality inference remain.
Stratigraphic-sedimentary evolution of a mixed siliciclastic-carbonate system in the Huizhou Sag of the Pearl River Mouth Basin, Northern South China Sea
Authors: Chen Weitao , Liu Jun , Peng Guangrong , Wei Zhe , Jia Peimeng , Yao Jiali , Wang Zhina , Zhuo Haiteng
Published: January 2024

Mixed siliciclastic-carbonate systems are regulated interactively by factors such as siliciclastic sediment supply, carbonate production, sea-level change, tectonism, and climate conditions. These systems record vital information that aids in understanding ancient environments. This study used a merged 3D seismic volume, in conjunction with over 100 industrial wells, to systematically investigate the stratigraphic-sedimentary evolution of such a system within the Huizhou Sag of the Pearl River Mouth Basin, located on the northern continental shelf of the South China Sea. In total, six major sequence boundaries were identified for the Zhujiang Formation within the area, thus subdividing the interval into five typical third-order depositional sequences. Each of these sequences can be divided into a transgressive and a highstand systems tract. Lowstand or falling stage systems tracts were also recognized, the deposition of which was potentially in response to the uplifting process of the Dongsha Rise. During the deposition of the Zhujiang Formation, the Huizhou Sag may have undergone a sequential evolutionary history from delta-shore, to delta-shore-tidal-lagoon, to delta-shore-carbonate, and finally to delta-shore-shallow marine systems. This evolution responded to a varying degree of mixing processes, which was mainly regulated by siliciclastic sediment supply, confined paleomorphology, and local oceanic currents. Furthermore, the deposition of the Zhujiang Formation in the Huizhou Area was time-equivalent with the spreading process after the ridge jump of the South China Sea (23–16.5 Ma), providing valuable insights into sea-level fluctuations, provenance changes, and tectonic evolution. Our results may also shed light on the evaluation of lithologic traps and hydrocarbon sweet spots within mixed siliciclastic-carbonate systems.
A cross-sectional analysis of the relationship between ischemic stroke and uric acid in young people in highland areas
Authors: Yang Yifan , Gao Li , Shen Fuwei , Miao Jiaodan , Liu Hua
Published: January 2024

Objective

To investigate the relationship between serum uric acid (SUA) levels and ischemic stroke in young patients in the Ganzi Tibetan plateau area.

Methods

A cross-sectional survey was undertaken from January 2020 to June 2023 involving young patients (age: 15–45 years) diagnosed with ischemic stroke. The survey was conducted at the Department of Internal Medicine of the People’s Hospital of Derong County, Ganzi Prefecture. The participants underwent a comprehensive assessment, including questionnaire surveys, physical examinations, laboratory tests, and head computed tomography (CT) examinations. Based on the tertiles of serum uric acid (SUA) levels, the patients were stratified into three groups. Furthermore, stroke severity was classified into mild (1–4 points), moderate (5–15 points), and severe (>15 points) categories using the National Institute of Health Stroke Scale.

Results

The severe stroke group exhibited higher levels of age, glucose, systolic blood pressure, serum triglyceride, low-density lipoprotein cholesterol, and serum uric acid (SUA) compared to the mild stroke group (P < 0.05). Furthermore, the likelihood of male sex, advanced age, smoking, and a family history of stroke, diabetes mellitus, and heart disease were significantly elevated in the severe stroke group compared to the moderate stroke group (P < 0.05). Multivariate logistic regression analysis conducted on young adults residing in highland areas revealed a significant association between SUA levels and the risk of stroke.

Conclusion

Elevated SUA levels serve as a distinct risk factor for the development of a major stroke in young patients in highland areas. At SUA levels of 320.56 mol/L, the risk of a moderate-to-severe stroke is noticeably elevated.
Potential disease biomarkers for diabetic retinopathy identified through Mendelian randomization analysis
Authors: Zou Xuyan , Ye Suna , Tan Yao
Published: January 2024

Background

Diabetic retinopathy (DR), a leading cause of vision loss, has limited options for effective prevention and treatment. This study aims to utilize genomics and proteomics data to identify potential drug targets for DR.

Methods

We utilized plasma protein quantitative trait loci data from the Atherosclerosis Risk in Communities Study and the Icelandic Decoding Genetics Study for discovery and replication, respectively. Genetic associations with DR, including its subtypes, were derived from the FinnGen study. Mendelian Randomization (MR) analysis estimated associations between protein levels and DR risk, complemented by colocalization analysis to examine shared causal variants.

Results

Our MR analysis identified significant associations of specific plasma proteins with DR and proliferative DR (PDR). Elevated genetically predicted levels of WARS (OR = 1.16; 95% CI = 0.095-0.208, FDR = 1.31×10-4) and SIRPG (OR = 1.15; 95% CI = 0.071-0.201, FDR = 1.46×10-2) were associated with higher DR risk, while increased levels of ALDOC (OR = 1.56; 95% CI = 0.246-0.637, FDR = 5.48×10-3) and SIRPG (OR = 1.15; 95% CI = 0.068-0.208, FDR = 4.73×10-2) were associated with higher PDR risk. These findings were corroborated by strong colocalization evidence.

Conclusions

Our study highlights WARS, SIRPG, and ALDOC as significant proteins associated with DR and PDR, providing a basis for further exploration in drug development. Additional studies are needed to validate these proteins as disease biomarkers across diverse populations.
Non-cooperative game-based electricity pricing method for data centers
Authors: Li Jianduo , Ye Guisen , Gao Feng
Published: January 2024

Data centers are characterized by high energy consumption, with operating costs being extremely sensitive to electricity prices. Therefore, modern data centers are often equipped with microgrids for power supply, which adjust their operational strategies based on the given electricity prices to minimize costs. However, existing research has overlooked the flexible pricing potential of electricity retailers, prompting this study to propose a non-cooperative game theory-based optimization method for data center electricity procurement negotiation. A two-layer optimization model is established for data center electricity pricing. The upper layer focuses on electricity price optimization, modeling price negotiation as a Stackelberg game and adopting a weighted average cost approach to describe the electricity procurement prices. The lower layer addresses data center operational optimization, formulated as a nonlinear programming problem. To enable rapid solution convergence, a hybrid problem-solving method combining a genetic algorithm and branch-and-cut algorithm is proposed. Finally, a simulation is conducted using a data center located in California, United States, to validate the proposed method. The results demonstrate that the proposed method can reduce data center operational costs by 3% and increase the revenue for electricity retailers by 17%, achieving a win–win outcome.
Solvent-free NMC electrodes for Li-ion batteries: unravelling the microstructure and formation of the PTFE nano-fibril network
Authors: Matthews G. A. B. , Wheeler S. , Ramírez-González J. , Grant P. S.
Published: January 2024

The microstructure and electrochemical performance of solvent-free processed and slurry cast Li(Ni0.6Co0.2Mn0.2)O2 (NMC622) based electrodes for Li-ion batteries has been investigated. In contrast to a moss-like PVDF-based carbon binder domain in slurry cast electrodes, the PTFE binder in solvent-free electrodes had a hierarchical morphology composed of primary fibrils of a few µm in diameter and 100’s µm in length that branched into secondary and then ever finer fibrils, down to diameters of 10s nm or below. A mechanism for the formation of the branch-like morphology observed in PTFE-based solvent-free electrodes is also presented. Even the finest fibrils were confirmed to survive typical cathode cycling conditions. The solvent-free electrodes showed progressive improvement in capacity with increasing charge-discharge rate (up to 150% at 2C) compared with slurry cast equivalents. The capacity of solvent-free electrodes faded 40% slower over 200 cycles at C/3. Impedance analysis showed the solvent-free microstructure enabled reduced charge transfer resistance and ionic resistance, arising from minimal obscuration of the active material surface and no pore blockage.