bluuebunny/crossref_metadata_2025 huggingface dataset:
Republished: Society for Neuroscience in Anesthesiology and Critical Care Expert Consensus Statement: Anesthetic Management of Endovascular Treatment for Acute Ischemic Stroke*
Authors: Talke Pekka O. , Sharma Deepak , Heyer Eric J. , Bergese Sergio D. , Blackham Kristine A. , Stevens Robert D.
Published: July 2014

Literature on the anesthetic management of endovascular treatment of acute ischemic stroke (AIS) is limited. Anesthetic management during these procedures is still mostly dependent on individual or institutional preferences. Thus, the Society of Neuroscience in Anesthesiology and Critical Care (SNACC) created a task force to provide expert consensus recommendations on anesthetic management of endovascular treatment of AIS. The task force conducted a systematic literature review (up to August 2012). Because of the limited number of research articles relating to this subject, the task force solicited opinions from experts in this area. The task force created a draft consensus statement based on the available data. Classes of recommendations and levels of evidence were assigned to articles specifically addressing anesthetic management during endovascular treatment of stroke using the standard American Heart Association evidence rating scheme. The draft consensus statement was reviewed by the Task Force, SNACC Executive Committee and representatives of Society of NeuroInterventional Surgery (SNIS) and Neurocritical Care Society (NCS) reaching consensus on the final document. For this consensus statement the anesthetic management of endovascular treatment of AIS was subdivided into 12 topics. Each topic includes a summary of available data followed by recommendations. This consensus statement is intended for use by individuals involved in the care of patients with acute ischemic stroke, such as anesthesiologists, interventional neuroradiologists, neurologists, neurointensivists and neurosurgeons.
A new species of Philodendron (Araceae) in Paraná, Brazil
Authors: Buturi Camila Vanessa , Temponi Lívia Godinho , Sakuragui Cassia Mônica
Published: July 2014

A new species of Philodendron from the Atlantic Forest in Paraná, Brazil, is described and illustrated. Philodendron meridionale belongs to subgenus Philodendron, section Macrobelium. It is similar to P. loefgrenii, from which it differs by the cordate base and larger number of locules, and is also similar to P. roseopetiolatum, from which it can be distinguished by smaller leaves and obscure primary lateral veins.
Hemiboea malipoensis, a new species of Gesneriaceae from southeastern Yunnan, China
Authors: ZHANG LiXiang , TAN YunHong , LI JianWu , WEN Bin , YIN JianTao , LAN QinYing
Published: July 2014

Hemiboea malipoensis, a new species of Gesneriaceae from southeastern Yunnan, China, is here described and illustrated. Based on morphological and molecular evidence, it was found to be similar to H. magnibracteata and H. cavaleriei var. paucinervis by conspicuous involucre and anthers fused by entire adaxial surfaces, but can be easily distinguished from the latter two species by its manificat spherical involucre, 3.5–4.5 cm in diameter, cymes with 4–10 flowers, corolla larger, 4.5–5.5 cm long, pale yellow-yellow, corolla tube glabrous, Calyx lanceolate, 5-parted from the base.
A new species of the genus Chamaecrista (Leguminosae, Caesalpinioideae) from Chapada dos Veadeiros, Goiás, Brazil
Authors: SILVA MARCOS JOSÉ DA , Souza Alessandro Oliveira
Published: July 2014

Chamaecrista frondosa from Brazil is described, illustrated, and discussed herein. This species is morphologically similar to C. psoraleopsis in its general leaflet shape and belongs to Chamaecrista sect. Absus series Microphyllae due to its caespitose, subshrubby habit, young branches, inflorescence axis, and pedicel with glandular trichomes, leaflets resin-dotted, ascending in the rachis and partly facing each other, as well as flowers with 10 fertile stamens. The species appears to be restricted to Cerrado sensu stricto and rocky fields in the Chapada dos Veadeiros National Park, an area with notable endemism. A key to the species of Chamaecrista sect. Absus series Microphyllae occurring in the region is provided.
Lectotypification of some names in the Jovibarba heuffelii group (Crassulaceae)
Authors: Nikolić Danijela , Kuzmanović Nevena , Walter Johannes , Lakušić Dmitar , Randelović Vladimir , Letz Dominik Roman
Published: July 2014

A taxonomic revision of the highly variable Jovibarba heuffelii group needs a proper typification of the names of the taxa described. The following names are lectotypified and shortly discussed: Diopogon stramineus, Sempervivum ciliatum, S. heuffelii, S. heuffelii var. glabrum, S. kopaonikense, S. patens and S. velenovskyi. Pictures of the type specimens are also provided. Other names recorded in the synonymy of J. heuffelii [Sempervivum brassaii Schur (1866: 229), S. heuffelii var. albanicum Kitanov (1948: 200), S. heuffelii var. bulgaricum Cheshmedjiev (1969: 474), S. heuffelii var. vestitum Domin (1933: 17), S. reginae-amaliae Heldreich & Sarnthein ex Baker (1877: 230), S. transylvanicum Baker (1874: 104)] are also commented.
A new combination in Asian Clinopodium (Lamiaceae)
Authors: Turner Ian
Published: July 2014

Clinopodium umbrosum (M.Bieb.) Koch (1849: 673) was originally described from Georgia. A broad view of this species, which ranges from the Mediterranean to East Asia, has been maintained by some authors employing this name (Cramer 1981, Leblebici 1982, Clement 1999) or one of its homotypic synonyms (e.g. Satureja umbrosa (M.Beib.) Scheele (1843: 577), Keng 1978). However, from the Himalayas eastward one or more different species have been recognised by others. The name C. repens (D.Don) Benth. in Wallich (1830: 66) has been used for the eastern element in two major Chinese floras (Wu & Hsuan 1977, Li & Hedge 1994), although it was not noted that this name was first validly used for a South American species, published by Vellozo in 1829 (Stafleu & Cowan 1988), the year before Bentham transferred Thymus repens Don (1825: 113) to Clinopodium. Clinopodium repens (D.Don) Benth. in Wallich (1830: 66) is therefore an illegitimate later homonym and cannot be used. The next available synonym appears to be Ziziphora javanica Blume (1826: 822), described from Java. As there is no combination in Clinopodium for this basionym, I propose a new combination below:
Morphological variability of new chrysophyte stomatocyst forming a single-cyst assemblage in a low-conductivity tropical lake in the Guineo-Congolian rainforest
Authors: Piątek Jolanta , Piątek Marcin
Published: July 2014

Morphological variation of the chrysophyte stomatocyst found in surface sediments of a small, shallow lake in the Guineo-Congolian rainforest in eastern Cameroon, is described, illustrated and discussed. Neither living cells nor scales of chrysophytes were observed in the lake. The stomatocyst cannot be assigned to any known cyst and is described as new to science (stomatocyst #49) using International Statospore Working Group (ISWG) guidelines. This is the third report of stomatocysts in Africa, and the second from the tropical part of this continent. Some basic ecological information on the sampling site is provided and compared with similar data from other sampling sites of chrysophytes in Africa.
A new subspecies of Crocus pestalozzae (Iridaceae) from Turkey
Authors: Yüzbaşıoğlu Sırrı , Özhatay Neriman
Published: July 2014

C. pestalozzae subsp. violaceus is described as a new subspecies from northwest Anatolia. The new taxa differs from subsp. pestalozzae by its flower colour, leaf and flower size, kartotype, habitat and growing elevation. Diagnostic characters, a full description, and detailed illustrations are presented. Also photograps of metephase plate, karyotypes and idiograms of two subsp. of C. pestalozzae are given. The geographical distribution of the subspecies is mapped. IUCN threatened category and observations on the population are noted.
Syzygium sahyadricum (Myrtaceae), a new tree species from India, and notes on the distribution of S. spathulatum Thwaites
Authors: Jose Robi Aloor , Puthiyapurayil Sujanapal , Nanu Sasidharan
Published: July 2014

A new species of the tree genus Syzygium (Myrtaceae), S. sahyadricum is described and illustrated from the Montane Shola forests of Anamalai and Palni Phytogeographical region of Western Ghats. Although phenotypically closely similar to S. spathulatum and S. malabaricum, the new species is easily recognizable by the pale yellow coloured tender leaves with horizontal secondary nerves and white flowers in reduced metabotryoid, pedunculate inflorescence, which are flattened towards the apex. Scanning the Syzygium collections in various herbaria revealed that similar specimens from various localities of this phyto-region are available and most of them with erroneous ascriptions. The report of S. spathulatum Thwaites, a Sri Lankan endemic species in India, was due to misinterpretation of Beddome’s collection. In this paper taxonomic peculiarities of the new species and allied taxa are discussed for better understanding.
Lectotypification and further notes on the identity of Commelina hirsuta, an endemic Indian species of Commelinaceae
Authors: Nampy Santhosh , Joseph Sheba M. , Madhavan Manudev K
Published: July 2014

The identity and nomenclature of the endemic Indian species Commelina hirsuta (Wight) Beddome (Commelinaceae) has puzzled taxonomists. This was further accelerated by the publication of a new name Commelina beddomei for this species recently.  In the present paper, the identity and nomenclature of Commelina hirsuta (Wight) Beddome is discussed. A lectotype is designated and additional notes on the species are provided.
A new species of Trocholejeunea (Lejeuneaceae, Marchantiophyta) from China
Authors: Wang Jian , Gradstein Stephan Robbert , Cheng Xia-Fang , Zhu Rui-Liang
Published: July 2014

Trocholejeunea sinensis J.Wang bis, R.L.Zhu & Gradst. sp. nov. (Lejeuneaceae) is described and illustrated based on specimens collected from Guangxi and Yunnan, China. The new species differs from all other species of Trocholejeunea by the presence of an “extra” lobule tooth situated at the extreme end of the free margin, near the junction of the lobule and the lobe. A key to the species of Trocholejeunea is provided.
Nomenclatural changes, new country records and range extensions of Baridinae (Coleoptera, Curculionidae) from China
Authors: PRENA JENS , YANG JIANI , REN LI , WANG ZHILIANG , LIU NING , ZHANG RUNZHI
Published: July 2014

Thirteen relatively frequently collected species of baridine weevils from mainland China were mapped to improve our understanding of biogeographic patterns and distributional ranges of Baridinae in the Palaearctic and Oriental regions. This simple case study showed that taxonomists tend to underestimate the spatial scope required in regional investigations and descriptive works often resulting in widespread species being described numerous times. Several apparently uncommon species with uncertain relationships, known previously only from moderately high latitudes in the Palaearctic region, showed Oriental affinities. In each region, species may occur over many thousands of kilometers not only in East-West but also in North-South directions. New synonyms are Mimophilus Faust (=Baridiomorphus Voss), Acythopeus inflatirostris Voss (=Acythopeus proximus Voss), Baris albisquama Zaslavskij (=Baris ljaodunensis Zaslavskij), Mimophilus tragicus Faust (=Baridiomorphus conicollis Voss), Moreobaris deplanata (Roelofs) (=Acythopeus patruelis Voss), Baris pilosa Roelofs (=Baris blennus Marshall, =Baris piliventris Zaslavskij, =Baris pygidialis Voss, =Baris suvorovi Reitter), Baris artemisiae (Panzer) (=Baris corvina Voss), Pellobaris melancholica (Roelofs) (=Paracythopeus collaris Voss), Pteridobaris maritima (Roelofs) (=Baris quinquecarinata Zaslavskij) and Ulobaris kuchenbeisseri Hartmann (=Baris pseudospitzyi Zaslavskij, =Baris ussuriensis ussuriensis Zaslavskij, =Baris ussuriensis chinganensis Zaslavskij). New combinations are Athesapeuta gracilis (Voss) (from Eumycterus Schönherr), Athesapeuta inornata (Voss) (from Baris Germar) and Nespilobaris inflatirostris (Voss) (from Acythopeus Pascoe). Baridius vestitus Perris (not Boheman) and Baris pygidialis Hustache (not Voss) are newly recognized primary homonyms. A lectotype is designated for Moreobaris deplanata. Twenty-four species are recorded newly from China.
Cheylostigmaeus tarae sp. nov. and Stigmaeus delaramae sp. nov. (Acari: Stigmaeidae) from Kurdistan, Iran
Authors: KHANJANI MOHAMMAD , NASROLLAHI SIAMAK , ZAMANI ALI SINA , FAYAZ BAHMAN ASALI
Published: July 2014

Two new species belonging to the family Stigmaeidae, Cheylostigmaeus tarae sp. nov. and Stigmaeus delaramae sp. nov., are described from specimens collected from soil and litter under pear trees, Pyrus communis L. (Rosaceae) in Iran. A key to all Iranian species of the genera Cheylostigmaeus (male) and Stigmaeus (female) are provided.
Pentad Evolution of Wintertime Impacts of the Madden–Julian Oscillation over the Contiguous United States
Authors: Baxter Stephen , Weaver Scott , Gottschalck Jon , Xue Yan
Published: July 2014

Lagged pentad composites of surface air temperature and precipitation are analyzed for the winter season (December–February) to assess the influence of the Madden–Julian oscillation (MJO) on the climate of the contiguous United States. Composites are based on the Wheeler and Hendon MJO index as well as an index developed and maintained at NOAA’s Climate Prediction Center (CPC), which is based on extended empirical orthogonal function analysis of upper-level velocity potential. Significant positive temperature anomalies develop in the eastern United States 5–20 days following Wheeler and Hendon MJO index phase 3, which corresponds to enhanced convection centered over the eastern Indian Ocean. At the same lag, positive precipitation anomalies are observed from the southern Plains to the Great Lakes region. Negative temperature anomalies appear in the central and eastern United States 10–20 days following Wheeler and Hendon MJO phase 7. These impacts are supported by an analysis of the evolution of 200-hPa geopotential height and zonal wind anomalies. Composites based on the CPC velocity potential MJO index generally yield similar results; however, they capture more cases since the index contains both interannual and subseasonal variability. There are some cases where the CPC index differs from that of WH in both MJO phase identification and its North American impacts, especially near the West Coast. This analysis suggests that MJO-related velocity potential anomalies can be used without the Wheeler and Hendon MJO index to predict MJO impacts.
Helicobacter pylori immunization and atopic dermatitis in South Italian children
Authors: Pedullà Marcella , Fierro Vincenzo , Del Tufo Ester , Alfano Rossella , Triassi Maria , Perrone Laura
Published: July 2014

Background

The epidemiological decrease of Helicobacter pylori (Hp) infection has been recently associated to the increase of several extra‐intestinal allergic disorders.

Objective

We investigated the role of specific Hp IgG production in the development of IgE or not IgE mediated food allergy (FA) in children affected by atopic dermatitis (AD).

Methods

From January 2010 to July 2013, 290 South Italian children, aged between 26 and 142 months, were consecutively referred to the Pediatric Clinic of the Pediatric Department at Second University of Naples and were diagnosed as affected by AD. The patients were classified in two groups on the basis of diagnosis of food allergy (88 FA affected and 202 not FA affected) and further divided on the basis of the diagnosis of atopy (63 IgE mediated and 23 not IgE mediated). Hp serum IgG was detected using an enzyme linked immunosorbent assay (ELISA) kit (Wampole® Helicobactor pylori IgG ELISA II, Wampole Laboratories, Cranbury, NJ) and Hp stool antigens using enzyme immunoassay (Premier Platinum HpSa plus, Cincinnati OH).

Results

We found a statistically significant higher prevalence of Hp serology positivity in not FA vs. FA AD‐affected children (p  = 0.032) and a significant inverse association between FA and Hp immunization (1/OR 0.32 95% CI 0.11–0.95). Further, we identified an absolute prevalence Hp serology positivity in not‐IgE‐mediated rather than in IgE‐mediated FA AD‐affected patients (p  = 0.0006).

Conclusion

We hypothesize that specific Hp IgG production could protect against the development of both FA and atopy in AD‐affected children.
Van der Waals epitaxy and characterization of hexagonal boron nitride nanosheets on graphene
Authors: Song Yangxi , Zhang Changrui , Li Bin , Ding Guqiao , Jiang Da , Wang Haomin , Xie Xiaoming
Published: July 2014

Graphene is highly sensitive to environmental influences, and thus, it is worthwhile to deposit protective layers on graphene without impairing its excellent properties. Hexagonal boron nitride (h-BN), a well-known dielectric material, may afford the necessary protection. In this research, we demonstrated the van der Waals epitaxy of h-BN nanosheets on mechanically exfoliated graphene by chemical vapor deposition, using borazine as the precursor to h-BN. The h-BN nanosheets had a triangular morphology on a narrow graphene belt but a polygonal morphology on a larger graphene film. The h-BN nanosheets on graphene were highly crystalline, except for various in-plane lattice orientations. Interestingly, the h-BN nanosheets preferred to grow on graphene than on SiO2/Si under the chosen experimental conditions, and this selective growth spoke of potential promise for application to the preparation of graphene/h-BN superlattice structures fabricated on SiO2/Si.
Facile synthesis and electrochemical performances of hollow graphene spheres as anode material for lithium-ion batteries
Authors: Yao Ran-Ran , Zhao Dong-Lin , Bai Li-Zhong , Yao Ning-Na , Xu Li
Published: July 2014

The hollow graphene oxide spheres have been successfully fabricated from graphene oxide nanosheets utilizing a water-in-oil emulsion technique, which were prepared from natural flake graphite by oxidation and ultrasonic treatment. The hollow graphene oxide spheres were reduced to hollow graphene spheres at 500°C for 3 h under an atmosphere of Ar(95%)/H2(5%). The first reversible specific capacity of the hollow graphene spheres was as high as 903 mAh g-1 at a current density of 50 mAh g-1. Even at a high current density of 500 mAh g-1, the reversible specific capacity remained at 502 mAh g-1. After 60 cycles, the reversible capacity was still kept at 652 mAh g-1 at the current density of 50 mAh g-1. These results indicate that the prepared hollow graphene spheres possess excellent electrochemical performances for lithium storage. The high rate performance of hollow graphene spheres thanks to the hollow structure, thin and porous shells consisting of graphene sheets.

PACS

81.05.ue; 61.48.Gh; 72.80.Vp
Pigeonpea genotypes influence parasitization preference and survival and development of the Helicoverpa armigera larval parasitoid, Campoletis chlorideae
Authors: Hugar Shiddalingappa V , Sharma Hari C , Basavan Goud Kondikallu
Published: July 2014

Studies were undertaken to identify pigeonpea,Cajanus cajan(L.) Millspaugh and the wild relative of pigeonpea,Cajanus scarabaeoides(L.) (accession ICPW 125,) genotypes that are hospitable to the pod borer,Helicoverpa armigera(Hübner) (Lepidoptera: Noctuidae) larval parasitoid,Campoletis chlorideaeUchida (Hymenoptera: Ichneumonidae) for the management of this pest in pigeonpea based cropping systems. Percentage parasitization of theH. armigeralarvae by theC. chlorideaefemales was greater under no-choice conditions than under multi-choice conditions because of forced parasitization under no-choice conditions. Lowest parasitization was recorded on the wild relative, ICPW 125, which may be due to long nonglandular hairs and low survival ofH. armigeralarvae. Parasitization ofH. armigeralarvae was greater under no-choice, dual-choice and/or multi-choice conditions on ICPL 87, ICPL 87119 and ICPL 87091, which are susceptible toH. armigera, than on the pod borer-resistant genotypes ICPL 332WR, ICPL 84060 and ICPB 2042; while survival and development of the parasitoid was better onH. armigeralarvae fed on ICPL 87, ICPL 87119, LRG 41, ICP 7035 and ICPL 87091 than on ICPL 332WR, ICPL 84060, ICPB 2042 and ICPW 125. The genotypes ICPL 87, ICPL 87119, LRG 42 and ICPL 87091 that are hospitable toC. chloridae, are better suited for use in integrated pest management to minimize the losses due toH. armigerain pigeonpea.
Evaluation of industrial dairy waste (milk dust powder) for acetone-butanol-ethanol production by solventogenic Clostridium species
Authors: Ujor Victor , Bharathidasan Ashok Kumar , Cornish Katrina , Ezeji Thaddeus Chukwuemeka
Published: July 2014

Readily available inexpensive substrate with high product yield is the key to restoring acetone-butanol-ethanol (ABE) fermentation to economic competitiveness. Lactose-replete cheese whey tends to favor the production of butanol over acetone. In the current study, we investigated the fermentability of milk dust powder with high lactose content, for ABE production by Clostridium acetobutylicum and Clostridium beijerinckii. Both microorganisms produced 7.3 and 5.8 g/L of butanol respectively, with total ABE concentrations of 10.3 and 8.2 g/L, respectively. Compared to fermentation with glucose, fermentation of milk dust powder increased butanol to acetone ratio by 16% and 36% for C. acetobutylicum and C. beijerinckii, respectively. While these results demonstrate the fermentability of milk dust powder, the physico-chemical properties of milk dust powder appeared to limit sugar utilization, growth and ABE production. Further work aimed at improving the texture of milk dust powder-based medium would likely improve lactose utilization and ABE production.
Expanded identification and characterization of mammalian circular RNAs
Authors: Guo Junjie U , Agarwal Vikram , Guo Huili , Bartel David P
Published: July 2014

Background

The recent reports of two circular RNAs (circRNAs) with strong potential to act as microRNA (miRNA) sponges suggest that circRNAs might play important roles in regulating gene expression. However, the global properties of circRNAs are not well understood.

Results

We developed a computational pipeline to identify circRNAs and quantify their relative abundance from RNA-seq data. Applying this pipeline to a large set of non-poly(A)-selected RNA-seq data from the ENCODE project, we annotated 7,112 human circRNAs that were estimated to comprise at least 10% of the transcripts accumulating from their loci. Most circRNAs are expressed in only a few cell types and at low abundance, but they are no more cell-type-specific than are mRNAs with similar overall expression levels. Although most circRNAs overlap protein-coding sequences, ribosome profiling provides no evidence for their translation. We also annotated 635 mouse circRNAs, and although 20% of them are orthologous to human circRNAs, the sequence conservation of these circRNA orthologs is no higher than that of their neighboring linear exons. The previously proposed miR-7 sponge, CDR1as, is one of only two circRNAs with more miRNA sites than expected by chance, with the next best miRNA-sponge candidate deriving from a gene encoding a primate-specific zinc-finger protein, ZNF91.

Conclusions

Our results provide a new framework for future investigation of this intriguing topological isoform while raising doubts regarding a biological function of most circRNAs.
Non-targeted transcription factors motifs are a systemic component of ChIP-seq datasets
Authors: Worsley Hunt Rebecca , Wasserman Wyeth W
Published: July 2014

Background

The global effort to annotate the non-coding portion of the human genome relies heavily on chromatin immunoprecipitation data generated with high-throughput DNA sequencing (ChIP-seq). ChIP-seq is generally successful in detailing the segments of the genome bound by the immunoprecipitated transcription factor (TF), however almost all datasets contain genomic regions devoid of the canonical motif for the TF. It remains to be determined if these regions are related to the immunoprecipitated TF or whether, despite the use of controls, there is a portion of peaks that can be attributed to other causes.

Results

Analyses across hundreds of ChIP-seq datasets generated for sequence-specific DNA binding TFs reveal a small set of TF binding profiles for which predicted TF binding site motifs are repeatedly observed to be significantly enriched. Grouping related binding profiles, the set includes: CTCF-like, ETS-like, JUN-like, and THAP11 profiles. These frequently enriched profiles are termed ‘zingers’ to highlight their unanticipated enrichment in datasets for which they were not the targeted TF, and their potential impact on the interpretation and analysis of TF ChIP-seq data. Peaks with zinger motifs and lacking the ChIPped TF’s motif are observed to compose up to 45% of a ChIP-seq dataset. There is substantial overlap of zinger motif containing regions between diverse TF datasets, suggesting a mechanism that is not TF-specific for the recovery of these regions.

Conclusions

Based on the zinger regions proximity to cohesin-bound segments, a loading station model is proposed. Further study of zingers will advance understanding of gene regulation.
Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study
Authors: Swamynathan Priyanka , Venugopal Parvathy , Kannan Suresh , Thej Charan , Kolkundar Udaykumar , Bhagwat Swaroop , Ta Malancha , Majumdar Anish Sen , Balasubramanian Sudha
Published: July 2014

Introduction

Mesenchymal stromal/stem cells (MSCs) for clinical use have largely been isolated from the bone marrow, although isolation of these cells from many different adult and fetal tissues has been reported as well. One such source of MSCs is the Whartons Jelly (WJ) of the umbilical cord, as it provides an inexhaustible source of stem cells for potential therapeutic use. Isolation of MSCs from the umbilical cord also presents little, if any, ethical concerns, and the process of obtaining the cord tissue is relatively simple with appropriate consent from the donor. However, a great majority of studies rely on the use of bovine serum containing medium for isolation and expansion of these cells, and porcine derived trypsin for dissociating the cells during passages, which may pose potential risks for using these cells in clinical applications. It is therefore of high priority to develop a robust production process by optimizing culture variables to efficiently and consistently generate MSCs that retain desired regenerative and differentiation properties while minimizing risk of disease transmission.

Methods

We have established a complete xeno-free, serum-free culture condition for isolation, expansion and characterization of WJ-MSCs, to eliminate the use of animal components right from initiation of explant culture to clinical scale expansion and cryopreservation. Growth kinetics, in vitro differentiation capacities, immunosuppressive potential and immunophenotypic characterization of the cells expanded in serum-free media have been compared against those cultured under standard fetal bovine serum (FBS) containing medium. We have also compared the colony-forming frequency and genomic stability of the large scale expanded cells. Secretome analysis was performed to compare the angiogenic cytokines and functional angiogenic potency was proved by Matrigel assays.

Results

Results presented in this report identify one such serum-free, xeno-free medium for WJ expansion. Cells cultured in serum-free, xeno-free medium exhibit superior growth kinetics and functional angiogenesis, alongside other MSC characteristics.

Conclusions

We report here that WJ-MSCs cultured and expanded in Mesencult XF, SF Medium retain all necessary characteristics attributed to MSC for potential therapeutic use.
Heme-Binding Properties of HupD Functioning as a Substrate-Binding Protein in a Heme-Uptake ABC-Transporter System in Listeria monocytogenes
Authors: Okamoto Yasunori , Sawai Hitomi , Ogura Mariko , Uchida Takeshi , Ishimori Koichiro , Hayashi Takashi , Aono Shigetoshi
Published: July 2014

The HupDCG protein complex is a putative ABC-transporter for heme in the pathogenic Gram-positive bacterium Listeria monocytogenes, where HupD functions as a heme-binding protein. UV–vis absorption, EPR, and resonance Raman spectroscopy have revealed that HupD binds a heme with two histidine residues as the axial ligands. His105 and His259 are identified as the axial ligands by site-directed mutagenesis. HupD is the first example of a heme-binding protein having a bis-histidine coordination environment among the heme-binding proteins working in bacterial heme acquisition systems. While mutation of His259 to Ala resulted in a loss in the heme-binding ability of HupD, the H105A variant of HupD retained its heme-binding ability with lower heme-binding affinity compared with the wild type. These results suggest that His259 is an essential ligand for heme acquisition by HupD and that His105 might be responsible for regulation of the heme-binding affinity of HupD during the heme-transport process.
Contractual Aspect of Division of Jurisdictions in the Russian Federation: History and Present
Authors: Chertkov Aleksandr , Kistrinova Olga
Published: July 2014

The article examines the meaning and role in the formation of Russian statehood contractual aspects of the delimitation of powers between the Russian Federation and its subjects. Analyzes the historical and political importance of the Federal treaty and agreements on the delimitation of powers between Federal bodies of state power and bodies of state power of the subjects of the Russian Federation, their role in the system of Russian law. The authors come to the conclusion that a twentyyear experience of the delimitation of competence led to the domination of the legislative differentiation of powers, and further perspectives of the contractual process. The article also treaties and agreements became an auxiliary mechanism designed to clarify certain issues of delimitation of competencies from a regional perspective. Their conclusion is justified only in exceptional cases, if it is conditioned by legally significant characteristics of the Russian Federation.
Limitations of Powers of Court of Cassation for Application of Part 6 Article 15 of the Criminal Code of the Russian Federation
Authors: Kravtsova Anastasiya
Published: July 2014

The article investigates the limits of power of the court of cassation instance on the application of Part 6 of Article 15 of the Criminal Code of the Russian Federation in the revision become enforceable judgments. Based on the analysis of criminal and criminal procedure norms the author comes to the following conclusions: Part 6 of Article 15 of the Criminal Code of the Russian Federation should be brought into conformity with paragraph 1 of Article 299 of Code of Criminal Procedure of the Russian Federation by a more precise definition of of procedural position of the person to may be applied this rule and streamlining sequence of actions court, in certain cases, the court of cassation may modify the judgment and apply the provisions of Part 6 of Article 15 of the Criminal Code of the Russian Federation.
Complicity in Commission of Administrative Offence
Authors: Morozova Natalya
Published: July 2014

The question of possible complicity of the offenders committing administrative violations is controversial. In the Code of Administrative Offences of The Russian Federation has no notion of complicity, at the same time an administrative offences can be committed by several persons, that is confirmed by the analysis of article 14.32 of the Code. In this article the author proposes to introduce institution of complicity by means of joint participation into Code of Administrative Offences of The Russian Federation using experience of the theory of criminal law. The article considers the possibility of complicity between two individuals, two artificial bodies or artificial body and an individual entrepreneur; and also between the artificial body and its employee — under certain conditions. The punishment for each of the participants shall be determined in accordance with its part in commission of the offence. The offence of complicity should be an circumstance aggravating responsibility.
Role Refinement in Access Control: Model and Analysis
Authors: Xia Hao , Dawande Milind , Mookerjee Vijay
Published: July 2014

Access control mechanisms in software systems administer user privileges by granting users permission to perform certain operations while denying unauthorized access to others. Such mechanisms are essential to ensure that important business functions in an organization are conducted securely and smoothly. Currently, the dominant access control approach in most major software systems is role-based access control. In this approach, permissions are first assigned to roles, and users acquire permissions by becoming members of certain roles. However, given the dynamic nature of organizations, a fixed set of roles usually cannot meet the demands that users (existing or new) have to conduct business.

The typical response to this problem is to myopically create new roles to meet immediate demand that cannot be satisfied by an existing set of roles. This ad hoc creation of roles invariably leads to a proliferation in the number of roles with the accompanying administrative overhead. Based on discussions with practitioners, we propose a role refinement scheme that reconstructs a system of roles to reduce the cost of role management. We first show that the role-refinement problem is strongly NP-hard and then provide two polynomial-time approximation algorithms (a greedy algorithm and a randomized rounding algorithm) and establish their performance guarantees. Finally, numerical experiments—based on a real data set from a firm's enterprise resource planning system—are conducted to demonstrate the applicability and performance of our refinement scheme.
The resurrection of the spectacle
Authors: Schallenberg André
Published: July 2014

One major topic of the European performance/avant-garde scene of the last century was 'reality', mostly accompanied by the contentious catchword 'post-dramatic' and work with lay people or 'experts'. However, productions that seemed political or revolutionary often revealed themselves to be nothing more than part of a marketing strategy by theatres aimed at unlocking new spaces of spectatorship. However, alongside these attempts, an alternative practice has emerged amongst young artists, who once again emphasise the artistic, subjective and ritual character of theatre, in order to bring it back to its origins. Using the example of three current productions, this article aims to show the means these artists work with and why this theatre is in its own way significantly more political than many alleged 'reality' productions.
From affective reading to the theory of affect: Reading / Feeling, Tanja Baudoin, Frédérique Bergholtz and Vivien Ziherl (eds.) (2013), Amsterdam: If I Can't Dance, I Don't Want To Be Part of Your Revolution
Authors: Založnik Jasmina
Published: July 2014

The reader Reading / Feeling is the result of a two-year art-theory research programme organised by the Dutch organization If I Can't Dance. Its innovation is not reflected in the selection of texts, but in the methodological approach of opening up a different (affective, paranoid) mode of reading. The compilation offers to a reader various entry points that are not to be taken in only rationally, but also experientially, to be precise, through an affective response.
Biopolitics, Deleuzianism and the destiny of aesthetics
Authors: Apostolou-Hölscher Stefan
Published: July 2014

In the second half of the eighteenth century, the emergence of a concept of 'life itself' as living not only has an impact on the economical and political fields but deeply touches on traditional understandings of the work of art as well. The birth of aesthetics within philosophy is just a consequence of, not the reason for, new practices and procedures in the field of art. Until now, the strict rules of Poetics had governed and distributed mimesis, keeping the different arts separate whilst subsuming their subjects under the idea of an organic unity. With the aesthetic and biopolitical turn in the second half of the eighteenth century, every measure is lost.
Sticky tales for affect: Episodes of situated thought
Authors: Caspão Paula
Published: July 2014

The article takes the form of a travelogue, embodying a method of geographically and affectively situated research and writing. It is an episode of documentation of a long-term journey of 'inadequate' study focusing on affect and theory, affect and writing, affect and place (affect and displacement). Convoking the notion of 'permeable ways of being' (Brennan, 2004), the text generates a half-theoretical, half-fictional discursive movement that doesn't go straight, reporting and reflecting on a recent collective artistic residency meant to study forms of life; on the very process of writing as cross-temporal and cross-geographical; on daily economies of affect that glide into affect morals.
Getting Affective
Authors: Kraigher Amelia
Published: July 2014

This issue focuses on the issues of affectivity and emotionality in contemporary theatre and on their (performative) theorisations. It draws from theorisations and/or case studies to develop diverse discussions about the mentioned aspects of the performing arts that also result from the modes of production themselves; in addition, it raises issues regarding emotions in the theatre, both in the past and today, and reflects on the manner in which theatre art is determined and driven by passions. It also addresses the phenomenon of the actor's fear of passion (convulsions, stress and the resulting burnout of artists…), the question of the (absence of) courage of theatre creators and theoreticians, which also includes a reflection on the history of stage fright, different trainings of actors' emotions, their objectives and methods; it reflects on passions from the aspect of theatre excess, the passion for prohibition and the question of conventions, on the authenticity of emotions in (contemporary) theatre and performance - doing all this in order to unfold the notion of passion as an issue of the political.
Authentic emotion and body-subject: Are these tears for real?
Authors: Kolenc Bara
Published: July 2014

The article discusses the phenomenon of aversion to exaggerated emotionality, characteristic of contemporary art. At the backdrop of historical subjectivity from the beginning of the twentieth century to the present day, defined by the dissolution of the authenticity of the Self and the emergence of a deconstructed, mediated subject, the author addresses the causes and conditions of this phenomenon. She tackles the questions of representation and the unrepresentable, which she traces through the aesthetic of realism and its demand for authentic emotions, modernism and its denial of indication and postmodern artistic practices, which are paradoxically defined not only by a general skepticism toward the authentic, but also toward the absolute inauthenticity of the raw mediation of signs and stand-in structures.
Affect and the performing arts: Biopolitics, transformation and potentiality
Authors: Bajda Anja
Published: July 2014

In this article the author explores affect within the representational framework, which is questioned as the paradigm of the theatrical convention. In relation to affective labour, physical presence and the real, being the core of theatrical illusion, it explores the emancipatory potential of performing arts conceived through the presence of affect. Actual virtuality becomes the key term with which to signify possibility, constituted within the creative act, as operative in the symbolic (field). The implications of the presence of affect within the performance are examined through its analytical introduction into the Slovenian theatrical performance Mandićmachine.
Address at the presentation of the Evergreen Crocodile Award
Authors: Repnik Vlado G. , Kardum Simon , Štromajer Igor
Published: July 2014

Address at the presentation of the Evergreen Crocodile Award for Lifetime Achievement to Dragan Živadinov - Slovenian theatre director and cosmonaut candidate, conceptual initiator of style formations retrogardism and telecosmism, founder of the Scipion Nasice Sisters Theatre, the Red Pilot Cosmokinetic Theatre and the Noordung Cosmokinetic Cabinet; author of the 50 year theatre projectile 1995-2045 - Noordung, Biomechanics Noordung, postgravitational theatrical abstracts and one of the three initiators of the Cultural Centre of European Space Technologies (KSEVT) in Vitanje - at the Borštnik Theatre Festival in Maribor on 19 October 2013.
The desire to be affected
Authors: Rozas Ixiar
Published: July 2014

In this article, the author connects affects with Lygia Clark's resonant body, with a story about Suely Rolnik and with the performing art piece Fissure no. 3: txoriak by Basque artist Idoia Zabaleta. In these three vignettes, the three authors and creators activate affects in a double movement: centripetal and centrifugal. To affect somebody, we first affect ourselves. And by doing so we change and alter others, according to Brian Massumi's theory of affects as something transindividual that goes beyond us. In this twofold movement, we can also make the familiar strange and the strange familiar, a condition that Kaja Silverman believes to be essential in creative work that considers itself political in today's context.
Beyond pathos: Interview with Annika Tudeer
Authors: Koch Christian
Published: July 2014

In this interview with Annika Tudeer, we pursue the question of possibility and the place of emotionality in contemporary theatre. At the forefront of the interview is the process-oriented practice of the Finnish theatre company OBLIVIA, which oriented itself very heavily around conceptual approaches of the 1970s through the 1990s but which in recent times has increasingly opened itself up by incorporating chance as a constructive principle for using affects and emotions as a medium of communication with the audience. In its more recent works, the company has allocated more room to that which one might call the pathos of acting. This serves a new performative aesthetics that endeavours to overcome the analytical approach to the theatre dispositive.
Periodismo y responsabilidad social: el paradigma del tratamiento informativo de la violencia contra la mujer
Authors: Martínez Rodríguez Beatriz
Published: July 2014

La comunicación es un derecho humano fundamental sancionado por la Declaración Universal de Derechos Humanos. Todos los seres humanos tienen derecho a expresar, comunicar o recibir libremente información veraz por cualquier medio de difusión. La actividad comunicativa es desempeñada por personas que actúan con una delegación colectiva de la sociedad, y el ejercicio de esa actividad está sometido a exigencias éticas, establecidas habitualmente mediante mecanismos de autorregulación. Estos deberes son particularmente acuciantes en el caso de los temas que preocupan a la opinión pública, sea por su gravedad, su extensión o la preocupación que generan entre los ciudadanos, como sucede en el caso de la violencia ejercida contra las mujeres. El objeto de este artículo es analizar el desarrollo de la investigación en torno a este tema en los medios de comunicación en España hasta la fecha; desentrañar cómo abordan hoy la cuestión de los malos tratos los medios de comunicación, y qué efectos ha tenido la extensa regulación desarrollada al respecto, incidiendo en las herramientas deontológicas implicadas en la tarea.
El profesional de los medios en el cine de los últimos años
Authors: Requeijo Rey Paula
Published: July 2014

Entre 1930 y 1999 se estrenaron en España 77 películas en las que el periodista juega un papel central. En el mismo período, el número de títulos asciende a 703 si incluimos aquéllos en los que simplemente aparece un profesional de los medios. Hay especialistas que hablan de un género o subgénero: el cine de periodistas. Desde 1975, encontramos a distintos autores que analizan la imagen del reportero en las películas (Maxwell Taylor, Alex Barris, Juan Carlos Laviana, Richard Ness, Larry Langman, Loren Ghiglione o Joe Saltzman). Lo habitual es que se centren en títulos emblemáticos desde los orígenes del cine hasta finales de los años 90. En el presente estudio, nos ocupamos de la representación del periodista en algunos de los largometrajes de la última década. Utilizamos el análisis de contenido y combinamos los modelos de la Teoría Profesional, la clasificación elaborada por el periodista Juan Carlos Laviana y la del profesor Joe Saltzman. El objetivo fundamental es determinar si hay algunas imágenes que predominan, cuáles son sus características, si junto a los MCM aparecen otros poderes y cómo se relaciona el reportero con ellos. En los cinco filmes analizados hay tres imágenes principales: la del periodista grato, la del comprometido y la del mánager. Aparecen representados el poder ejecutivo, el económico, el judicial y el de las fuerzas y cuerpos de seguridad. En unos casos se muestra lo positivo de la colaboración entre poderes y en otros la relación es negativa.
Empresarias malagueñas de Pymes y ‘social media optimization’ (optimización de los medios de comunicación sociales)
Authors: Belmonte Jiménez Ana María
Published: July 2014

En la Jornada “Buenas prácticas de empresas laborales constituidas por mujeres socias trabajadoras”, que tuvo lugar el 24 de febrero de 2010 en Málaga, las empresarias de pymes comentaban la importancia del uso de las tic en la empresa, mediante el uso de las redes sociales, el marketing y la comunicación. Algunos estudios como los de Cocktail Análisis (2010) configuran un perfil de las mujeres con respecto a la tecnología que va casi siempre por detrás del hombre; en el presente artículo, con respecto al “Social Media Optimization” y las mujeres, y tomando como muestra las entrevistas realizadas a cinco empresarias, se investiga sobre si dicha concepción de la mujer y las tecnologías se corresponde con la realidad. A tenor de los resultados obtenidos por las empresarias entrevistadas, las páginas web de las empresarias tienen una baja implementación del “Social Media Optimization”. El desconocimiento del SMO, sus herramientas y sus beneficios son las causas principales citadas por las cuales las empresarias no implementan el SMO.
Consumo del enoturismo en la D.O. Ribera del Duero
Authors: Matellanes-Lazo Mónica
Published: July 2014

En los últimos años, la palabra enoturismo, se ha incorporado definitivamente a nuestro vocabulario. Se trata de una nueva forma de turismo temático de carácter rural y con el vino como eje fundamental. En líneas generales, por enoturistas se entiende a las personas aficionadas al vino (no profesionales) que tienen un interés cultural hacia este producto y aprovechan vacaciones o tiempo libre para conocer zonas vitivinícolas, visitar bodegas, aprender los procesos de la elaboración e incluso vivir cerca la experiencia de la vendimia. La investigación que se muestra tiene por objetivo conocer el perfil del turista del vino en la D.O. Ribera del Duero y conocer sus gustos y preferencias de consumo del enoturismo. Para ello se ha realizado un cuestionario descriptivo para la obtención de los objetivos marcados. Los resultados aportan en crecimiento de las visitas a las bodegas de la D.O. Ribera del Duero y la preferencia por las catas y comidas en bodega.