bluuebunny/crossref_metadata_2025 huggingface dataset:
Occurrence of the Indo-West Pacific starfish Luidia magnifica (Echinodermata: Asteroidea) in the Mexican Pacific and a possible introduction to the Caribbean region
Authors: Galván-Villa Cristian Moisés , Solís-Marín Francisco Alonso , Lopez Karen , Cobb Janessa C. , Díaz-Pérez Leopoldo , Rezende-Ventura Carlos R. , Slivak Nataly , Ford Colby T. , Janies Daniel A.
Published: August 2023

The starfish Luidia magnifica (Asteroidea: Paxillosida: Luidiidae) is one of the biggest multiradiate starfish in the world that occurs in the Philippines, French Polynesia, and the Hawaiian Islands. Since its description in 1905 by Fisher, very few observations of this species have been documented therefore, limited information is available on this species. We present new shallow-water records for the Mexican Pacific obtained between 2017 and 2021. A total of 15 specimens of L. magnifica were recorded in the bay of Manzanillo, Colima, central Mexican Pacific. The specimens were collected manually, preserved, and deposited in two biological collections in Mexico: Biological Collection of the Laboratorio de Ecología Molecular, Microbiología y Taxonomía (LEMITAX), Universidad de Guadalajara and in the National Collection of Echinoderms "Dra. M. E. Caso" Instituto de Ciencias del Mar y Limnología (ICML), Universidad Nacional Autónoma de México (UNAM). A morphological description of specimens collected and additional ecological data is provided. Molecular techniques were applied to verify species identification. This record represents a considerable expansion of the known distribution range of L. magnifica and provides the first evidence of the presence of this species in the tropical Eastern Pacific. The presence of L. magnifica larvae from the Caribbean coast of Panama was also identified from DNA sequencing, indicating that it is possible that the species also occurs in this region. Because observations of the starfish in the Eastern Pacific have been recent, we assume the presence of larvae in the Caribbean is a recent introduction, possibly in ballast water of ships crossing the Panama Canal from the Eastern Pacific to the Western Atlantic.
Association between Platelet Count and Admission of Intensive Care Unit in Maternal Sepsis
Authors: Huang Yuwen , Lv Cheng-tian , Wang Yin-ling , Gao Yuanmei
Published: August 2023

Background: Maternal sepsis is a major cause of gestational morbidity worldwide. Due to physiological adjustment after pregnancy, it is challenging to identify whether there is association between platelet count (PLT/PC) and severity of maternal sepsis. Therefore, we aimed to find out the correlation between PLT/PC and Intensive Care Unit (ICU) admission and risk threshold of PLT/PC in maternal sepsis while deterioration.
Methods: We retrospectively distinguished 144 patients who were diagnosed as maternal sepsis in Third Affiliated Hospital of Guangzhou Medical University from September 4, 2018, to May 11, 2022. Information about demographic, laboratory, maternal and fetal outcome were collected through electronic medical record system in our hospital. Subjects were divided into three groups according to PLT/PC. To compare outcomes between them, a multivariate logistic regression analysis was conducted.
Results: There were 20.8% (30/144) patients whose PLT/PC was ≤100 (×109/L), 83.3% of them (25/30) were admitted into ICU. 51.4% (74/144) of subjects had a PLT/PC of 100-200 (×109/L), 62.2% (42/74) of them gained the admission of ICU. After adjusting for confounders, in the PLT/PC of ≤100 group, the odd of admission into ICU was more 16.8 times than in the reference group (PLT/PC of 100-200) (p＜0.05).
Conclusions: We should be warned whether there were deterioration when the PLT/PC was ＜100×109/L in maternal sepsis.
WITHDRAWN: A Multi-Objective Optimization Model for Energy Allocation and Petroleum Products Distribution in Green Supply Chains (Case Study of IRAN National Petroleum Corporation MIANDOB)
Authors: Attaria Mahdi Yousefi Nejad , Ejlaly Babak , Ghahvechia Fatemeh
Published: August 2023

The oil industry plays an important role in global energy demand. Oil and other fossil fuels have been a major cause of environmental and global warming for decades. Many industries need oil as a major energy source, and it is important to minimize the environmental impact of oil( minimizing greenhouse gas (GHG)) and maximize its benefits Petroleum Revenue Management Act (PRMA). The environmental impact of the oil industry is the emission of gases such as carbon dioxide, sulfur dioxide, and nitrogen oxide, which directly and indirectly affect the environment.So it is important, creating a green supply chain network(GSCN) model that pursues several goals.This study presents a multi-objective optimization model for energy allocation and petroleum product distribution in green supply chains.For this purpose a multi-objective mixed-integer programming (MIP) model is formulated to develop. The proposed model is designed a green supply chain(GSC) to reduce oil company costs and greenhouse gas(GHG) emissions under uncertain demand. We solve the proposed model with GAMS and present a case study to IRAN National Petroleum Corporation(INPC) MIANDOAB demonstrate the applicability and exhibit efficacy of the proposed model.
Insight into energy production and consumption, carbon emissions and agricultural residues resources available for energy and environmental benefits in China
Authors: Ji Li-Qun
Published: August 2023

China is encountering two big challenges: supply shortage of energy especially liquid fuels and serious environmental problems especially increasing CO2 emissions. Agricultural residues are readily available and inexpensive renewable resources in China and it can be expected to reduce China’s reliance on fossil fuels and CO2 emissions through the use of agricultural residues. This study conducted a systematic study of energy production and consumption, CO2 emissions, and agricultural residues available for energy and environmental benefits. The gap between the domestic production and consumption of energy in China became larger and larger, and China’s energy consumption structure was dominated by coal over the past thirty years. The increasing energy consumption especially coal consumption were strongly linked the increasing CO2 emissions in China. As the largest agricultural country in the world, China has abundant agricultural residues, most of which were utilized as soil amendments, animal feeds, substrates in mushroom cultivation, and industrial materials. The usable agricultural residues for energy were estimated to be about 226.9 million tons in 2020. If those usable agricultural residues would be converted into bio-oil and biochar through pyrolysis, it could not only replace about 102 million tons of crude oil, but also sequestrate about 209 million tons of CO2-equivalent. It is expected that the results from this study can promote the application of agricultural residues in potential of energy production and carbon sequestration.
Relationship between intertwin delivery interval and postpartum hemorrhage in cesarean section: a seven-year retrospective cohort study
Authors: Lan Yehui , Pan Shuangjia , Zhou Yujia , Chen Baoyi , Lu Xinyue , Hua Ying
Published: August 2023

Background: Intertwin delivery interval (ITDI) is defined as the interval time between the delivery of the first and the second twin and may be controlled by obstetricians during cesarean deliveries. Several studies have demonstrated the relationship between ITDI and the adverse neonatal outcomes during vaginal births. However, few studies have explored the relationship between ITDI and maternal outcomes in cesarean deliveries. We hypothesized that ITDI may influence the occurrence and the development of postpartum hemorrhage by affecting uterine contraction. The purpose of this study was to investigate the relationship between ITDI and postpartum hemorrhage, and between ITDI and the maternal adverse outcomes that related to the blood loss in women with twin pregnancies delivered by cesarean section.
Methods: A retrospective cohort of 1649 pregnant women with diamniotic twins was recruited from 2016 to 2022. They were categorized into the short ITDI group (0 min < ITDI ≤ 3 min), the medium ITDI group (3 min < ITDI ≤ 6 min) and the long ITDI group (6 min < ITDI ≤ 9 min) according to the range of ITDI of the eligible individuals (0-9 min). The primary outcome including PPH and the secondary outcomes including uterotonic administration, emergency operation, severe maternal morbidity and total blood loss ≥ 1500 ml were compared among the groups, and the relationship between ITDI and these outcomes was explored.
Results: Little differences were found in the rates of postpartum hemorrhage, emergency operation, severe maternal morbidity and total blood loss ≥1500 ml among the three groups (P > 0.05). No relationship was found between ITDI and postpartum hemorrhage (P > 0.05). Women with long ITDI were at a decreased risk of uterotonic administration (adjusted OR = 0.369, 95% CI = 0.228-0.597, P < 0.001).
Conclusions: Although the long ITDI did not reduce the incidence of postpartum hemorrhage during the cesarean delivery, the uterotonic administration was decreased though appropriately prolonged ITDI.
PET-CT as an Early Detection Method for Tuberculosis: A Systematic Review and Meta-Analysis
Authors: Yayan Josef , Rasche Kurt , Franke Karl-Josef , Windisch Wolfram , Berger Melanie
Published: August 2023

This meta-analysis aims to provide a deep analysis of the use of an imaging technique that combines positron emission tomography (PET) and computed tomography (CT) as a potential early detection method for tuberculosis (TB). Tuberculosis remains a significant global health challenge, and early detection is crucial for effective treatment and control. PET-CT offers unique advantages in the detection and characterization of TB lesions. This meta-analysis discusses the current literature, including the methodologies, results, and implications of using PET-CT in the early diagnosis of tuberculosis. The analysis of the studies revealed that the sensitivity of PET-CT as a potential method for the early detection of TB was 82.6%.
Proximal Policy Optimization (PPO)-Based Resource Allocation for Energy Harvesting Industrial Wireless Sensor
Authors: Li Rongzhen , Xu Lei , Tang Chengming , Wang Ping , Liu Wanli , Gu Junjie , Cai Zhicheng , Jiang Rui
Published: August 2023

For the purpose of overcoming the challenges of charging wireless sensors in the complicated industrial environment, researchers are concentrating more and more on sensor networks that can harvest energy.This paper looks at a wirelessly powered industrial sensor network where each sensor harvests energy from a specific radio frequency (RF) energy source and uses it to transmit data to a receiver.Two working modes are discussed of in this paper.One is the frequency division multiplexing (FDM) working mode, where the sensor simultaneously transmits data over orthogonal frequency bands while harvesting RF energy.Time division multiplexing (TDM), which divides each time slot into two successive intervals, is the second working mode.Data is transmitted and energy is harvested in the same frequency band, but at distinct intervals.Because the channel condition and energy harvesting process are unpredictable, an efficient resource allocation algorithm is required for the sensors.We propose a novel resource allocation algorithm based on reinforcement learning.The proposed algorithm achieves continuous resource allocation and is applicable for continuous states by using Proximal Policy Optimization (PPO).We also utilize entropy regularization, online normalization of state, reward scaling, and advantage normalization to improve the performance of resource allocation algorithm in real-world scenarios.In both FDM and TDM working modes, the proposed algorithm outperforms the greedy algorithm and random algorithm in terms of long-term throughput, according to the results of numerical simulations.
Paper 1: What is the environmental footprint of a dental practice; A Life Cycle Analysis
Authors: Duane Brett , Steinbach Inge
Published: August 2023

Introduction:
Growing awareness of environmental sustainability is essential. The IPCC's report stresses urgent carbon reductions to limit global warming to 1.5°C. The NHS in all four countries of the UK aims to be net-zero. Dental practices must prioritize sustainability for public health. This paper uses Life Cycle Analysis (LCA)where possible to study the dental practices' environmental footprint.

Methodology:
LCA methodology was used where possible to calculate the carbon footprint of a full-time dental clinic operating 220 days/year. Ecoinvent database and OpenLCA software were used to calculate GHG emissions, normalized factors, and DALYs. Attributional and spend-based approaches were used to analyze procurement items.

Results:
Compared with our 2015 paper, the 2023 carbon footprint, which used more current impact factors shows reduced water use emissions and increased total waste emissions. Staff travel and patient travel continues to significantly impact the carbon footprint. 99% of an average dental practice's health impact is from climate change.

Discussion:
Addressing waste and promoting low-carbon transport within the dental practice is crucial. Procurement's impact requires detailed analysis. Normalized scores highlight the environmental impact compared to an average person.

Conclusion:
The carbon footprint of a dental practice was updated using more current impact factors, to reinforce the high contribution of travel within the dental CFP, with an increase in the CFP of waste, and a reduction in the CFP of water
Effects of interdisciplinary teaching on student learning and healthcare-giving competence: A mixed methods study
Authors: Yang Bao-Huan , Lo Kao-Wen , Li Yuh-Shiow , Chao Kuo-Yu
Published: August 2023

Background
Interdisciplinary collaboration is known to foster professional innovation and enhance student learning across different domains. However, the research on the effectiveness of interdisciplinary teaching in pediatric nursing education and its evaluation methods is limited. Therefore, this study aimed to examine the effectiveness of incorporating game-based learning from the Department of Childcare and Education into therapeutic play courses in pediatric nursing education for students’ learning and healthcare-giving competence.
Methods
We used a mixed methods study with pre-test/post-test quantitative analyses and a descriptive qualitative analysis of students’ reflection journals. Data were collected between August 2019 and July 2020 to gauge course effectiveness. Quantitative data obtained from the questionnaires were analyzed using a t-test, correlation, and regression analysis. Qualitative data were analyzed using content analysis. One hundred and three second-year students enrolled in a four-year nursing program. Students attended classes in university classrooms and served learning in a kindergarten or hospital in Northern Taiwan.
Results
Implementing interdisciplinary teaching in the therapeutic play course resulted in significant improvement in students’ knowledge, abilities, attitudes, and social service outcomes as measured by post-test scores. Furthermore, the scores on the Service-Learning Questionnaire indicated a significant increase in communication, problem-solving, knowledge application, and cross-cultural competence. Integrating instruction to improve problem-solving skills and knowledge application predicts student learning outcomes. Qualitatively, students reflected on integrating different learning areas, communication, problem-solving, and practical knowledge skills.
Conclusions
Interdisciplinary teaching effectively stimulates thinking among nursing students and enhances their knowledge, abilities, and attitudes toward therapeutic play, benefiting children’s health. Integrating social service with interdisciplinary instruction allows students to be physically exposed to each child's unique challenges and needs, enhancing their communication, knowledge application, and problem-solving. Students can blend knowledge and skills through integrated learning, which is crucial for career development.
Trial Registration:
N/A. It was a survey on educational activities.
Biomass and metabolite production via hairy root culture using different genotypes of Panax ginseng
Authors: Nguyen Van Binh , Kim Min Jung , Vo Ngoc Linh Giang , Lee Yun Sun , Jayakodi Murukarthick , Park Hyun-Seung , Mohanan Padmanaban , Kang Kyo Bin , Ryu Byeol , Park Eun Jin , Park Tae Kyu , Yang Tae-Jin
Published: August 2023

Panax ginseng is an important medicinal plant containing many pharmacologically valuable ginsenosides, the biosynthesis of which is regulated by complex metabolic pathways. In vitro tissue culture is an alternative way to produce ginseng root biomass and metabolites. The hairy root production via transformation using root inducing plasmid (Ri plasmid) of Agrobacterium rhizogenes would be a useful metabolic engineering tool for P. ginseng. Here, we investigated the transformation efficiency and biomass production in five ginseng genotypes. Of these genotypes, the ‘Yunpoong’ cultivar, and the local landrace ‘Ganghwa’ had the highest transformation efficiencies of 66.11% and 65.00%, respectively. The biomass production of transgenic hairy roots was 1.5–2.1 times faster than that of non-transgenic adventitious roots without hormone supplementation. Various ginsenosides such as Rg1, Rf, Rh1, Rb1, Rb2, Rd, F2, and Rg3, were present at similar or higher levels in the hairy roots compared with the contents in adventitious roots. Further, the ginsenoside contents of cultivated roots were similar to those cultured in a bioreactor. Our results thus provide the basic information to metabolic engineering of ginseng for the in vitro production of ginsenosides.
A Systematic Evaluation of Time of Concentration (TC) Models in a Predominantly Agrarian Catchment of Southeastern Nigeria
Authors: Nnaji Chidozie Charles , Siwobi Tochukwu Raphael
Published: August 2023

Time of concentration (Tc) is a parameter in runoff estimation, used to study and design different types of hydraulic structures in watersheds. Any error in Tc calculation leads to an inaccurate estimation of the discharge, which can lead to over-sizing or under-sizing of designed facilities that can have great economic and environmental consequences. Therefore, choosing the correct method to estimate Tc for a specific catchment is of great importance. This study was aimed at comparing the applicability of fifteen carefully selected equations of time of concentration to Ifite Ogwari Catchment in Anambra State Nigeria. The catchment was first delineated in QGIS and all the requisite parameters for estimating the time of concentration as per the selected equations determined. Afterwards the selected equations were employed to obtain the Tc values for each sub-catchment. The calculated values of Tc were then subjected to exploratory statistical analysis and sensitivity analysis. Results showed that the Temez, Haktanir & Sezen and Chow equations gave the most representative of values of times of concentration of the sub-catchments with 100% of all Tc values obtained using these equations fell within the inclusion criteria of median ± 0.5 SD while returning the median Tc three times each out of eleven catchments. The Pasini and Sheridan models were the worst performing of all the fifteen equations evaluated with all the Tc values calculated using these equations fall outside median ± 0.5 SD and median ± SD. The Pasini and Sheridan models generally showed signs of gross Tc overestimation such that the values of Tc obtained using these two equations are about 3 to 4 times higher than the median values. Sensitivity analyses indicate that 82% of the equations consider the longest flow path as the most important catchment parameter while the equations of Dooge and Pilgrim & Mc Dermont consider catchment area as the most important catchment parameter. Slope was the least important parameter in all fifteen equations investigated for application in the study area.
Understanding the organisation and delivery of health service following the repatriation of South Sudanese refugees from the West Nile districts in Uganda
Authors: Komakech Henry , Atuyambe Lynn , El-Jardali Fadi , Orach Christopher Garimoi
Published: August 2023

Low-and-middle-income countries (LMICs) face overwhelming challenges providing health services to their populations, and even more for provision of health services to displaced populations. Little is known about how health services are organized and delivered to displaced populations in these countries especially following repatriation.
Objective
To examine the organization and delivery of health services following the repatriation of South Sudanese refugees in Uganda from three west Nile districts of Arua, Adjumani, and Moyo.
Methods
We conducted a qualitative case study in three West Nile refugee hosting districts of Arua, Moyo, and Adjumani. We used the World health Organization Health System Framework focusing on four blocks: health services, financing, medicines, and supplies and human resources. We conducted in-depth interviews with 32 purposefully selected respondents representing health service managers, district civil leaders, staff from local government and international aid agencies, and health service providers across primary, secondary, and tertiary levels of care. Content analysis was conducted.
Results
Following repatriation, the District Health Teams in the three districts assumed overall responsibility for planning, management, and provision of health services. Health service delivery was based on an integrated model. Health facilities provided comprehensive health services based on a decentralized framework in all the three districts. In addition, health services were available in most areas except for former refugee settlements where facilities were either closed or relocated. Post repatriation, the main source for health financing was government funding through the Primary Health Care grant with limited support from aid agencies. Districts, however, faced several challenges in health service delivery including shortage of medicines and essential supplies, inadequate health workers, and poor infrastructure.
Conclusion
The repatriation of refugees affected health services delivery in the refugee affected districts notably reduction in financial resources, availability of skilled human resources, equipment and as well as closure of some health facilities. Key stakeholders should plan and prepare for refugee repatriation and put in place mechanisms to support the continuity of health services delivery in refugee affected settings. Further research to examine health systems adaptability and resilience following repatriation is recommended.
Three-Dimensional Covalent Organic Frameworks with nia Nets for Efficient Separation of Benzene/Cyclohexane Mixtures
Authors: Fang Qianrong , Chang Jianhong , Chen Fengqian , Li Hui , Suo Jinquan , Zheng Haorui , Zhang Jie , Wang Zitao , Zhu Liangkui , Valtchev Valentin , Qiu Shilun
Published: August 2023

The synthesis of three-dimensional (3D) covalent organic frameworks (COFs) with highly connected building blocks presents a significant challenge. In this study, we report two novel 3D COFs with the nia topology, named JUC-641 and JUC-642, by introducing planar hexagonal and triangular prism nodes for the first time. The resulting materials display high crystallinity, excellent thermal/chemical stability, a through-hole structure, and a large specific surface area. Notably, our adsorption studies and breakthrough experiments reveal that both COFs exhibit exceptional separation capabilities, surpassing previously reported 3D COFs and most porous organic polymers, with a separation factor of up to 2.02 for benzene and cyclohexane. Additionally, dispersion-corrected density functional theory analysis suggests that the outstanding performance of these 3D COFs can be attributed to the incorporation of highly aromatic building blocks and the presence of extensive pore structures. Consequently, this research not only expands the diversity of COFs but also highlights the potential of functional COF materials as promising candidates for environmentally-friendly separation applications.
Lithium chloride treatments in free flying honey bee colonies: efficacy, brood survival and within colony distribution
Authors: REIN Carolin , BLUMENSCHEIN Marius , TRAYNOR Kirsten , ROSENKRANZ Peter
Published: August 2023

The efficacy of various Lithium Chloride (LiCl) applications in eradicating the parasitic mite Varroa destructor in honey bee colonies was investigated, with a specific focus on its impact on brood development. In broodless colonies (3 weeks post queen caging), the highest mite mortality rate of 98% was achieved with a 9-day treatment of 2.5 kg of 50 mM LiCl fondant candy. A shorter 5-day treatment with 2 kg of 50 mM LiCl candy resulted in a mite mortality rate of 77.5%. In colonies with brood, a repeated short-term application of 4*0.5 kg 50 mM LiCl candy yielded an efficacy of 88%. LiCl led to a removal of the first reared brood after release of the queen. However, no long-term effects on colony growth were observed, and the colonies successfully overwintered. Additionally, the study demonstrated that Lithium is rapidly distributed among the bees of a colony within 2 days, yet only low concentrations were detected in stored food samples. This suggests that the bees efficiently absorb and distribute LiCl within the colony. The harvested honey in the following spring revealed a lithium concentration of 0.1–0.2 mg/kg, which is below naturally occurring lithium levels in honey. Based on these findings, LiCl can be considered an effective and easy-to-apply acaricide in broodless colonies, and even in colonies with brood, it had no long-term effects on colony survival. Further research may be necessary to determine the optimal treatment period for achieving an efficacy over 95%.
Does MPK4/12-HT1 function as a CO2/bicarbonate sensor to regulate the stomatal conductance under high CO2 Levels?
Authors: Gahlowt Priya , Tripathi Durgesh Kumar , Singh Samiksha , Gupta Ravi , Singh Vijay Pratap
Published: August 2023

Elevated atmospheric carbon dioxide (CO2) concentrations have a serious detrimental impact on both plants as well animals (Gojon et al. 2022). In particular, plants grown in high CO2 concentrations exhibit reduced contents of proteins, minerals, and nutrients (Ebi and Loladze 2019). The adverse effects of elevated CO2 have been reported on global food quality and quantity along with negative impacts on human health (Ebi and Loladze 2019; Jacobson et al. 2019). Therefore, identification and understanding of the CO2 sensing mechanisms in plants are of critical importance to avoid adverse consequences of high atmospheric CO2 levels.
Exploring the potential of Aloe-vera extract as a non-hazardous electrolytic solution for batteries
Authors: Kale Avinash , Jain Arihant , Kakde Satyam , Hede Krushna , Chopra Swamini , Pande Kavita , Deshmukh Abhay
Published: August 2023

Electrical energy plays a major role in our daily life and its consumption is also unlimited. There are various ways to store the electricity generated, most common of which is the use of acid batteries and portable dry batteries. The disposal of these batteries creates environmental hazard and are toxic to humans handling them as well. The aim of this research is to develop a non-hazardous, environment friendly battery using a non-conventional, botanical source: Aloe-vera plant. Aloe-vera leaves are known to contain acid, which has the potential to generate electric current. Thus, the potency of Aloe-vera extract was studied as an electrolytic solution to generate electricity through metal electrodes (Copper and Zinc), similar to a conventional acid battery. The pulp from Aloe-vera leaves was extracted in two different states; one with the outer cover intact and another was pure pulp from the inner layer. It was observed that though the initial voltage for both the states of Aloe-vera extract was same at 0.17 V, the voltage generated after 30 minutes of charging by pure pulp extract was approx. double than that of the extract with outer cover intact. In another iteration, both the types of Aloe-vera extract were mixed individually with concentrated sulphuric acid and distilled water and the output voltage was compared. It was noted that in all the iterations, the pure Aloe-vera pulp proved to be more efficient, while the addition of sulphuric acid gave highest voltage, ~ 33% more than pure pulp electrolyte. The future of this work is to develop a higher capacity battery and to scale down the present set-up.
Bioprospecting of Ten Microalgae Species Isolated from Saline Water Lake for Evaluation of the Biodiesel Production
Authors: Khosravinia Sara , Malekzadeh-Shafaroudi Saeid , bagheri Abdolreza , Behdad Assieh , Moshtaghi Nasrin
Published: August 2023

Algal bioprospecting in ecosystems leads to exploring native microalgae and the competency evaluation of economically producing lipids as biofuel or nutritional applications. In this study, ten microalgae species were screened from the saltwater lake. Chlorella vulgaris, Chlorella sorokiniana, Chlamydomonas raudensis, Chlamydomonas hedleyi, Dunaliella salina, Picochlorum bazangan sp. nov., Tetraselmis bazangan sp. nov., Haematococcus lacustris, Nannochloropsis oceanic, and Scenedesmu rubescens were isolated and identified using 18SrDNA and tufA markers. Biodiesel potentials were assayed by the determination of biomass productivity, biochemical components, fatty acid profile, and biodiesel properties. The results showed that the maximum biomass yield (1.22 gL-1) belonged to C. vulgaris. The highest protein, carbohydrate, chlorophyll, and carotenoid content were recorded in C. vulgaris, C. raudensis, C. sorokiniana, and D. salina, respectively. N. oceanica accumulated high lipid content and omega-3 fractions (31.09%). However, C. hedleyi had the highest lipid productivity (11.64 gL-1d-1) compared to other microalgae. The best species for biodiesel production was C. vulgaris, with a specific growth rate of 0.36d-1, lipid productivity of 7.45 gL-1d-1, and C16-C18 fatty acid profile of 78.3%. The microalgae C. vulgaris had appropriate biodiesel properties of low viscosity (4.49), high cetane number (55.38), and relatively low cloud point (4.98). Another choice was N. oceanic, with high lipid productivity, cetane number (59.79), oxidative stability (56.43), and low iodine value (47.11). Microalgae T. bazangan sp. nov. had a cetane number (55.24), low cloud point (4.71), and C16-C18 fatty acid profile of 82.34%. Accordingly, C. vulgaris, T. bazangan sp. nov., and N. oceanic can be considered potential species for biodiesel.
Median and Medial umbilical ligament repositioning for prevention of pelviperineal complications following abdominoperineal resection – A case series and novel technique
Authors: Thomas Anand , TS Subi , Sleeba Teena , Antony Abhijith , george Naveen
Published: August 2023

Introduction:
Pelviperineal complications after abdominoperineal resections are tough to treat. None of the available prophylactic methods has proven efficacy besides being technically challenging and expensive to perform. The present study aims to describe the technical details and short-term outcomes using mobilized umbilical ligaments to cover the pelvic inlet.
Technique:
After completing the rectal resection, the bladder with umbilical ligaments is mobilised anteriorly into the space of Retzius until the free edge can reach the sacral midline. Hitching stitches are taken to fix the umbilical ligaments into the new position. Seven consecutive patients had the umbilical ligament flap used for pelvic inlet closure.
Results
Cross-sectional imaging on day 30 demonstrated the viable flap in all patients, and the small bowel descent was prevented. None of the seven patients had small bowel obstruction till day 90 after the operation. No patient required re-catheterization, experienced major complications, or wound infections that would necessitate re-intervention.
Conclusion
The use of mobilised umbilical ligaments hitched to the pelvic inlet is a technically safe and feasible procedure to prevent pelviperineal complications after APR.
The Genomic Ghosts of Geukensia granosissima
Authors: Wares John P.
Published: August 2023

Observations that biodiversity - particularly in coastal marine species - is redistributing itself as the environment changes has been a key indicator of the climate change era. Historical surveys help us identify such patterns in taxa that might not be typically monitored for distribution or abundance. As recently as 1992, the southern ribbed mussel Geukensia granosissima was considered to be relatively abundant in northeastern Florida. Climate velocity predictions might suggest that these mussels would now have cryptically invaded the marshes of Georgia, which has historically only harbored the congener G. demissa, with possible ecosystem consequences. I re-surveyed Geukensia from both coasts of Florida and sites in Georgia for mitochondrial and nuclear genomic diversity. As of this report, G. granosissima is all but gone from the Atlantic coast of Florida, though some signature of its history remains in these data. The reason for this rapid fluctuation in distribution is not known, but is important for clarification of regional biogeographic patterns.
Gut resistome linked to Sexual Preference and HIV infection
Authors: Garcia Elisa Rubio , Casadellà Maria , Parera Mariona , Vila Jordi , Paredes Roger , Noguera-Julian Marc
Published: August 2023

Background:People living with HIV (PLWH) are at increased risk of acquisition of multidrug resistant organisms due to higher rates of predisposing factors. The gut microbiome is the main reservoir of the collection of antimicrobial resistance determinants known as the gut resistome. In PLWH, changes in gut microbiome have been linked to immune activation and HIV-1 associated complications. Specifically, gut dysbiosis defined by low microbial gene richness has been linked to low Nadir CD4+ T-cell counts. Additionally, sexual preference has been shown to strongly influence gut microbiome composition in PLWH resulting in differentPrevotellaorBacteroidesenriched enterotypes, in MSM (men-who-have–sex-with-men) or no-MSM, respectively. To date, little is known about gut resistome composition in PLWH due to the scarcity of studies using shotgun metagenomics. The present study aimed to detect associations between different microbiome features linked to HIV-1 infection and gut resistome composition.Results:Using shotgun metagenomics we characterized the gut resistome composition of 129 HIV-1 infected subjects showing different HIV clinical profiles and 27 HIV-1 negative controls from a cross-sectional observational study conducted in Barcelona, Spain. Most no-MSM showed aBacteroides-enriched enterotype and low microbial gene richness microbiomes. We did not identify differences in resistome diversity and composition according to HIV-1 infection or immune status. However, gut resistome was more diverse in MSM group,Prevotella-enriched enterotype and gut micorbiomes with high microbial gene richness compared to no-MSM group,Bacteroides-enriched enterotype and gut microbiomes with low microbial gene richness. Additionally, gut resistome beta-diversity was different according to the defined groups and we identified a set of differentially abundant antimicrobial resistance determinants based on the established categories.Conclusions:Our findings reveal a significant correlation between gut resistome composition and various host variables commonly associated with gut microbiome, including microbiome enterotype, microbial gene richness, and sexual preference. These host variables have been previously linked to immune activation and lower Nadir CD4+ T-Cell counts, which are prognostic factors of HIV-related comorbidities. This study provides new insights into the relationship between antibiotic resistance and clinical characteristics of PLWH.
Small size anterolateral bundle graft and decreased posterior slope angle of tibia are the risk factors associate with postoperative posterior tibial translation after double-bundle posterior cruciate ligament reconstruction
Authors: Morita Yudai , Tajima Takuya , Yamaguchi Nami , Yokoe Takuji , Nagasawa Makoto , Ota Tomomi , Ouchi Kouki , Chosa Etsuo
Published: August 2023

Posterior tibial translation (PTT) after double-bundle posterior cruciate ligament (PCL) reconstruction has sometimes occurred. Purpose of this study is to identify the risk factors for postoperative PTT after double-bundle PCL reconstruction with a hamstring autograft. Comparing the results of bilateral gravity sag view (GSV) at 12 months after surgery, over 5-mm PTT was defined as ‘failure’ in this study. Of 26 isolated PCL reconstruction cases, over 5-mm PTT was seen in 7 cases (group F: 9.57 ± 1.28 mm), and 19 cases had less than 5 mm (group G: 2.84 ± 1.29 mm). Age, sex, body mass index (BMI), preoperative GSV, posterior slope angle of the tibia, anterolateral bundle (ALB) and posteromedial bundle (PMB) graft diameters, and tibial tunnel diameter were evaluated. The two groups were compared with the 2x2 chi-squared test, the Mann Whitney U-test, and Spearman’s rank correlation coefficient. Multivariate logistic regression analysis was also performed to determine the risk factor. Statistical significance was indicated as p < 0.01 for correlation with postoperative PTT, and as p < 0.05 for all other comparisons. Mean age (group G 31.8 ± 12.5 vs group F 34.9 ± 15.9 years), sex (male/female: 15/4 vs 3/4), BMI (25.6 ± 4.6 vs 24.9 ± 3.9 kg/m2), preoperative GSV (11.3 ± 2.2 vs 11.6 ± 2.9 mm), PMB diameter (5.37 ± 0.33 vs 5.36 ± 0.48 mm), and tibial tunnel diameter (9.32 ± 0.58 vs 9.29 ± 0.49 mm) showed no significant differences. ALB diameter was significantly greater in group G (7.0 ± 0.5 mm) than in group F (6.5 ± 0.29 mm; p = 0.022). There was also a significant difference in posterior tibial slope angle (9.19 ± 1.94 vs 6.54 ± 1.45, p = 0.004). On Spearman rank correlation coefficient analysis, ALB diameter GSV (correlation coefficient: -0.561, p = 0.003) and posterior tibial slope angle (correlation coefficient: -0.533, p = 0.005) showed a significant correlation with postoperative PTT. Multivariate logistic regression analysis showed that ALB diameter (OR, 19.028; 95%CI, 1.082–334.6; p = 0.044) and posterior slope angle of tibia (OR, 3.081; 95%CI, 1.109–8.556; p = 0.031) were independently associated with postoperative PTT, respectively. In double-bundle PCL reconstruction with hamstring, smaller ALB graft diameter and lower (flatted) tibial slope angle were considered risk factors for postoperative PTT.
Research on Intelligent Recognition Technology in Lithology Based on Multi- parameter Fusion of Logging While Drilling
Authors: Liang Haibo , Xiong Jiaguo , Yang Yi , Zou Jialing
Published: August 2023

In oil and gas drilling, timely and accurate identification of formation lithology is an important guarantee of drilling safety. Aiming at the problems of inaccurate identification of lithology in drilling by traditional methods, and low efficiency due to the fact that even modern instruments cannot respond to lithology in real time. the Categorical Boost (CatBoost) model was applied to lithology identification in this study. However, since CatBoost uses more hyperparameters in its modeling, it is difficult to optimize model prediction by manually tuning the parameters. Therefore, the introduction of Kernel Principal Component Analysis (KPCA) extracts fewer and more important features from the original data, eliminates the redundant information contained therein, and combines with Bayesian Optimization (BO) algorithm to optimize the hyperparameters during the training process, thus improving the prediction performance of CatBoost. Two experiments were designed to verify the recognition ability of the model, and the final test results of the model showed that the KPCA-BO-CatBoost model proposed in this paper had the best overall performance, and the lithology recognition accuracy reached more than 90%. The model was effective in identifying the formation lithology, realized real-time lithology identification by combining the parameters of logging while drilling, improved the efficiency and accuracy of lithology identification, and was of great significance in guiding the subsequent drilling work.
Application of continuous nursing under the health belief model in prophylactic colostomy for rectal cancer
Authors: Sun Lin , Lu Jing , Wang Jiacheng , Zhang Hailing , Chu Aiqin
Published: August 2023

Background: To explore the effects of continuous nursing based on the health belief model on the rehabilitation of patients undergoing anterior rectal resection and preventive colostomy.
Methods: From September 2020 to October 2021, 80 patients who met the inclusion criteria for preventive enterostomy were randomly divided into the observation and control groups. Patients in both groups were followed up for 3 months after discharge from the hospital. The control group was given routine follow-ups, and the observation group was given continuous nursing intervention under the guidance of the health belief model. Adaptability to colostomy, colostomy self-efficacy, colostomy complications, quality of life after colostomy, and defecation control ability after colostomy were observed in the two groups.
Results: There were no significant differences in sex, age, tumor stage, tumor location, or educational level between the two groups (P > 0.05). Three months after intervention, colostomy adaptability and colostomy self-efficacy scores in the observation group were higher than those in the control group (132.38±8.33 and 101.26±9.4, respectively). The quality of life scores after colostomy in the observation group were higher than those in the control group at 1 month (49.2±6.99) and 3 months (59.85±5.16) after discharge, and the incidence of postoperative complications in the observation group was 20% lower than that in the control group (P < 0.05). The scores of defecation control ability at 1 month (21.25±11.05) and 3 months (17.475±9.808) after colostomy in the observation group were lower than those in the control group (P< 0.05).
Conclusion: Continuous nursing interventions based on the health belief model can improve colostomy adaptability, colostomy self-efficacy, quality of life, and defecation function after colostomy in patients with rectal cancer undergoing anterior rectal resection with prophylactic colostomy.
Mechanisms associated with the development of atrial fibrillation after sepsis and the role of neuregulin-1
Authors: Kang Wen , Deng Jingru , Fan Zheru , Zhou Fang , Wang Xi , Liu Kang , Wang Long
Published: August 2023

Purpose
To explore the mechanisms involved in the development of atrial fibrillation (AF) after sepsis and examine the effect of neuregulin-1 (NRG-1) on AF and related mechanisms.
Methods
We used cecal ligation and puncture (CLP) to establish the sepsis model. NRG-1 was administered via the tail vein at a dose of 0.01µg/g 12 and 24 h postoperatively to determine its effect on AF after sepsis.
Results
Compared with Sham rats, septic rats exhibited enhanced AF inducibility, atrial fibrosis, norepinephrine (NE), and C-reactive protein (CRP) levels, reduced action potential duration (APD), atrial effective refractive period (AERP), acetylcholine (Ach) levels, expression of Nav1.5, Cav1.2, and Kv1.5, and significantly decreased INa, ICa, L, and Ikur current densities. We observed that NRG-1 could reduce APD, atrial fibrosis, levels of CRP and NE, INa and ICa, L current densities, and expression levels of Nav1.5 and Cav1.2, however, it failed to prevent the onset of AF. Compared with the Sham group, the Sham + NRG-1 group rats showed a reduction in APD, AERP, INa and ICa, L current densities, Nav1.5 and Cav1.2 expression levels, elevated AF inducibility, Ach levels, Ikur current density, and Kv1.5 expression.
Conclusion
Sepsis can induce tissue and electrical remodeling in the atria and promotes the development of AF. NRG-1 could attenuate the degree of atrial fibrosis and organismal inflammation in sepsis while promoting the development of AF in Sham rats, impacting atrial electrophysiology and ionic currents.
"iNETgrate": integrating DNA methylation and gene expression data in a single gene network
Authors: Zare Habil , Sajedi Sogand , Ebrahimi Ghazal , Roudi Raheleh , Mehta Isha , Samimi Hanie , Kazempour Shiva , Zainulabadeen Aamir , Docking Thomas , Arora Sukeshi , Cigarroa Francisco , Seshadri Sudha , Karsan Aly
Published: August 2023

Integrating multi–omics data in one model can increase statistical power. However, designing such a model is challenging because different omics are measured at different levels. We developed the iNETgrate package (https://bioconductor.org/packages/iNETgrate/) that efficiently integrates transcriptome and DNA methylation data in a single gene network. Applying iNETgrate on five independent datasets improved prognostication compared to common clinical gold standards and a patient similarity network approach.
Green Synthesis of Nickel (Ni) Nanoparticles using Garlic Extract, Characterizations, and their Antimicrobial Activities
Authors: Kakar Natasha Saleem , Kasi Jafar , Bilal Ahmed , Akbar Ali , kasi Ajab , Ahmed Shehzad , Ali Syed Wajahat
Published: August 2023

The green synthesis route for production of nickel nanoparticles using garlic extract is being reported for first time. In the synthesis of Ni nanoparticles, garlic extract was used as reducing agent and nickel nitrate as a precursor salt and were characterized for their size, morphology, crystallinity and their effects on bacterial and fungal strains were investigated. Nanoparticles size was ranging from 20 to 130 nm and shapes were cylindrical and spherical confirmed by scanning electron microscope (SEM). X-ray diffraction (XRD) proved the crystalline nature and purity of nanoparticles. Ni nanoparticles were found effective against Escherichia coli, Salmonella typhi, Staphylococcus aureus and Klebsiella pneumoniae. The antibacterial activity was observed by measuring the diameter of inhibition zone using nanoparticles. Furthermore, nanoparticles have shown the potential to inhibit the growth of fungus such as Aspergillus nigerand Mucor mucedo. This novel antimicrobial potential is helpful for application in food industries and market.
Formal volunteering, informal volunteering and cognitive function among Chinese older adults
Authors: Liang Siya , Zhang Jiayu , Ye Zhicheng , Lan Ting , He Jiayi , Lin Wenyi
Published: August 2023

Background:
While the benefits of volunteering have been well-documented in the current literature, it does not tend to distinguish between various types of volunteering activities and the association between volunteering and cognitive function among Chinese older adults remains unclear. This study aims to examine association of volunteering and cognitive function among Chinese older adults and identify whether volunteering can be an effective intervention for dementia.
Method:
The 2018 China Health and Retirement Longitudinal Study (CHARLS) data were used for analysis. The generalized linear regression models are used to estimate the associations between volunteering type and cognitive function among Chinese older adults aged 60 and above (N=2370). Also, this study replaced the dependent variable to test the robustness of the regression results.
Results:
Informal volunteering does not significantly affect the cognitive function of Chinese older adults, while formal volunteering has a significant positive effect on Chinese older adults’ cognitive function. These findings are robust after replacing the dependent variable.
Conclusions:
This study provides evidence that formal volunteering can improve cognitive function among Chinese older adults, whereas informal volunteering can not, indicating that formal volunteering can be en effective intervention to protect against the decline of cognitive function. Therefore, formal volunteering should be incorporated into behavioral health intervention promgrams to help reduce the prevalence of cognitive impairment among Chinese older adults.