bluuebunny/crossref_metadata_2025 huggingface dataset:
PERANCANGAN SISTEM DIGITAL MARKETING UMKM BARONGAN SEBAGAI UPAYA PERLUASAN TARGET PASAR DAN PENGENALAN BARONGAN KHAS BLITAR
Authors: Arum Dewi Puspa , Adnin Hani Nur , Novitasari Dewi , Nurhalizah Lilis , Andriana Yeyen , Prakoso Bagastya Budi
Published: April 2024

Banyak generasi muda yang kurang peduli dengan budaya di Indonesia, dengan melihat permasalahan tersebut, pengabdian ini dilakukan untuk memperkenalkan budaya barongan khas Blitar kepada khalayak luas sekaligus membantu memperluas target pasar produk barongan. Dengan adanya pengabdian masyarakat yang dilakukan, kegiatan ini dapat memberikan kontribusi serta manfaat dalam meningkatkan daya saing dan memperluas target pasar Barongan Wisanggeni Art Gallery. Di setiap daerah memiliki jenis barong yang berbeda – beda, contohnya seperti di Blitar, barongan blitar sering disebut sebagai Barongan Kucingan. Barongan Khas Blitar memiliki karakter tegas, galak namun suka bercanda atau bermain yang dikenal dengan sifat “Ngucing”. Bahan baku pembuatannya berasal dari kayu yang ukurannya besar dan berat. Metode yang dilakukan yaitu dengan cara melakukan observasi dan wawancara untuk mengetahui kondisi dan keadaan yang ada pada UMKM Barongan selanjutnya kami melakukan identifikasi permasalahan untuk mengetahui dengan detail permasalahan yang sedang dihadapi UMKM Barongan yaitu kurang luasnya target pasar karena belum dikenalnya barongan khas Blitar ke khalayak luas. Setelah mengetahui permasalahan, kami melakukan penyelesaian masalah dengan menggunakan metode SOSTAC (Situation analysis, Objectives, Strategy, Tactics, Actions, and Control) sebagai alat ukur keberhasilan kami. Dalam proses pengabdian ini, penulis berhasil membuat sistem digital marketing dengan menyediakan berbagai plaftrom online seperti Shopee, TikTok, Instagram, dan Youtube sebagai media promosi, membuatkan konten yang menarik pelanggan, serta mendampingi dan memberikan pelatihan kepada pelaku atau pekerja UMKM Wisanggeni Art Gallery.
PENDAMPINGAN SERTIFIKASI HALAL DAN NIB BAGI UMKM DI KELURAHAN RUNGKUT TENGAH, GUNUNG ANYAR, KOTA SURABAYA
Authors: Dwi Putri Dita Keysia Armelia , Muna Ervia Okta Izzatul , Astuti Nur Habibah Dwi , Wijaya Rega Putra , Simatupang Sarah Regina
Published: April 2024

Kelurahan Rungkut Tengah berada di Kecamatan Gunung Anyar, Kota Surabaya. Terdapat berbagai jenis Usaha Mikro Kecil Menengah (UMKM) di Kelurahan Rungkut Tengah mulai dari makanan,minuman, aksesoris, dan yang lainnya.Pemerintah menetapkan untuk memangkas proses pembuatan perizinan berusaha bagi pelaku udaha mikro dan kecil sepertinyang tertulis dalam Perppu 2/2022 tentang cipta kerja.Untuk perizinan berusaha UMKM yang memiliki tingkat resiko rendah cukup memiliki NIB.Selain NIB, sertifikat halal juga menjadi bagian penting dalam produk UMKM makanan dan minuman. Setelah melakukan pendataan,mahasiswa kelompok 90 KKN-Tematik UPN “Veteran” Jawa Timur menemukan ada banyak UMKM yang belum memiliki izin legalitas yang di akui oleh pemerintah. Dengan hasil survey tersebut, kelompok 90 KKN-Tematik UPN “Veteran” Jawa Timur bersama kelompok UMKM Maju Mapan mengadakan kegiatan sosialisasi program NIB (Nomor Induk Berusaha) dan sertifikasi halal di Kelurahan Rungkut Tengah yang dihadiri oleh para pelaku UMKM di Kelurahan Rungkut Tengah.Selain itu,dilakukan juga pendampingan pembuatan NIB melalui Online Single Submission (OSS).Kemudian data NIB tersebut kemudian digunakan dalam proses pendampingan untuk mendapatkan sertifikasi halal.
INOVASI EKSTRAK DAUN KENIKIR (COSMOS CAUDATUS KUNTH) SEBAGAI PEWARNA ALAMI PADA MIE BASAH
Authors: Purwasih Unik , Pramuswara M. Galih
Published: April 2024

Penelitian ini berjudul Inovasi Ekstrak Daun Kenikir (Cosmos Caudatus Kunth) Sebagai Pewarna Alami Pada Mie Basah. Masalah yang diteliti adalah bagaimana proses Inovasi Ekstrak Daun Kenikir (Cosmos Caudatus Kunth) Sebagai Pewarna Alami Pada Mie Basah. Kenikir adalah tanaman jenis sayuran di indonesia yang banyak hidup di daerah tropis yang sering dikonsumsi sebagai lalapan dan obat tradisional yang memiliki kandungan yang cukup baik, sebagai antioksidan, antikanker, anti jamur, anti-inflamasi dan anti mikroba. Tujuan dari penelitian ini adalah untuk mengkaji lebih lanjut dari pemanfaatan daun kenikir sebagai pewarna alami dalam pembuatan mie basah. Penelitian ini menggunakan metode eksperimen dimulai dengan pembuatan ekstrak daun kenikir kemudian dilanjutkan dengan pembuatan mie.  Hasil Penelitian menunjukan bahwa daun kenikir selain memiliki manfaat kandungan yang baik tapi juga mampu digunakan sebagai inovasi bagi masyarakat yang tidak menyukai sayuran. Penambahan zat warna yang alami ini merupakan salah satu cara yang efisien dalam pengelohan sayuran. dalam hal ini ekstrak daun kenikir ini dapat digunakan pada bahan tambahan makanan lainnya dengan metode yang berbeda. Ekstrak daun kenikir yang digunakan sebagai pewarna alami pada pembuatan mie basah menghasilkan warna yang khas dan aroma yang tidak memengaruhi kualitas dari mie yang dihasilkan. Mie yang dihasilkan memiliki tekstur yang kenyal dan sama seperti mie pada umumnya.
BEYOND BANKING: INVESTING IN PROSPERITY - THE ROLE OF BANKS IN FINANCIAL PLANNING AND WEALTH MANAGEMENT
Authors: Dave Riddhi , Sanghavi Preyal
Published: April 2024

Financial planning and wealth management is becoming crucial and omni present. In this paper the authors have tried to deliberate on rising significance of financial planning and wealth management, impact of covid-19 pandemic on financial planning and wealth management, recent trends in financial planning and wealth management, SWOT Analysis of the financial planning and wealth management industry at Global Level and Indian Level, role of Banks in financial planning and wealth management and rising use of Technology by banks in catering to Financial Planning and Wealth Management, all these with the aim to buttress the presence of banks in this industry which is offering humungous growth potential.
LIQUIDITY AND PROFITABILITY ANALYSIS OF THE SELECTED IT COMPANIES OF INDIA
Authors: Patel Anjali , Ramanuj Jaydeep
Published: April 2024

For any organisation ratios are extremely useful tools for understanding financial statements and making decisions. For sustainability and long-term future growth, short and long-term success are critical to every company. As IT companies are becoming the major player in not only in one county but all over world. The study's major goal is to have better understand of the relationship between the liquidity ratio and profitability ratio for two selected Indian IT companies: TCS (Tata Consultancy Services) and Infosys. For the study data is collected from the selected companies' annual reports for the duration of 10-year period, from March 2014 to March 2023. For statistical analysis purpose statistical methods, arithmetic mean and regression analysis were used. The liquidity ratio shows that TCS outperformed Infosys, as does the profitability ratio. Regression analysis results shows that there is no connection between the liquidity ratio and the profitability ratio.
Gambaran Penggunaan Insulin Pada Pasien Diabetes Melitus Tipe II Rawat Jalan Di Salah Satu Rumah Sakit Negeri Tabanan Bali
Authors: Ratnasari Pande Made Desy , Prasetya Anak Agung Ngurah Putra Riana , Arini Heny Dwi
Published: April 2024

Intisari

Penyandang Diabetes Melitus Tipe 2 (DMT2) dengan kontrol glikemik yang buruk berdampak pada peningkatan morbiditas dan mortalitas sehingga diperlukan penggunaan insulin secara dini. Terdapat dua golongan insulin berdasarkan asalnya (manusia dan analog) yang memiliki keunggulan masing-masing, maka dari itu penelitian ini bertujuan untuk mengetahui gambaran penggunaan insulin pada pasien DMT2 rawat jalan di salah satu Rumah Sakit Negeri Tabanan Bali.

Penelitian ini menggunakan rancangan cross sectional yang dilaksanakan pada bulan Desember 2019 dengan teknik purposive sampling berdasarkan kriteria inklusi yaitu pasien DMT2 rawat jalan yang memperoleh insulin yang sama selama minimal 3 bulan kunjungan, berumur >18 tahun dan data rekam medik yang lengkap. Pengumpulan data berdasarkan rekam medik pasien selama periode September-November 2019 menggunakan instrumen penelitian. Data dianalisis secara deskriptif menggunakan software Microsoft Excel yang ditampilkan dalam bentuk tabel.

Penelitian ini melibatkan 88 pasien yang mayoritas berumur 46-65 tahun (53,4%), berjenis kelamin laki-laki (53%), tidak bekerja (60%), berpendidikan SD (57%), mengalami DM <5 tahun, 90% mengalami komplikasi mikrovaskuler serta 80% mengalami penyakit penyerta. Seluruh pasien menggunakan insulin analog dengan jenis insulin kerja panjang (49%) yang telah sesuai dengan pedoman terapi. Pengunaan insulin analog lebih banyak digunakan karena memiliki beberapa keunggulan yaitu efek samping hipoglikemia lebih rendah dan dapat menurunkan HbA1c hingga 2% sedangkan insulin kerja panjang memiliki lama kerja yang panjang yaitu 12-24 jam sehingga mempermudah dalam waktu penyuntikan serta hampir tidak memiliki efek puncak sehingga dapat meminimalkan terjadinya efek hipoglikemia.
Evaluasi Terapi Aspirin dan Non Aspirin Terhadap Nilai PTT aPTT Pada Kejadian Stroke Berulang di RSUD Dungus Madiun
Authors: Nawangsari Palupi
Published: April 2024

Stroke adalah manifestasi klinik dari gangguan fungsi serebral, baik fokal maupun global, yang berlangsung cepat selama lebih dari 24 jam, atau berakhir dengan kematian tanpa ditemukan penyebab selain daripada gangguan vaskular. Pencegahan stroke berulang salah satunya, menggunakan terapi antiplatelet sebagai pengencer darah. Tujuan penelitian ini adalah mengetahui gambaran, perbandingan perbedaan terapi aspirin dan non aspirin pada kejadian stroke berulang di RSUD Dungus Madiun. Jenis penelitian adalah Desain deskriptif kuantitatif dengan metode cohort pada data rekam medis, hasil laboratorium PTT aPTT. Jumlah data dan sampel sebanyak 10 pasien.

Karakteristik pasien dengan jenis kelamin yang mengalami stroke didominasi 60% laki-laki 40% perempuan pada rentang usia 55-64 tahun sebanyak  50%, riwayat merokok sebanyak 60%  dan dengan penyakit penyerta terbanyak yaitu hipertensi dan hiperlipidemia 40%. Pengunaan terapi aspirin sebanyak 50% dan non aspirin sebanyak 50%. Terapi aspirin maupun non aspirin tidak terdapat perbedaan yang signifikan terhadap nilai PTT aPTT, tidak terdapat kejadian stroke berulang pada penggunaan terapi aspirin maupun non aspirin.
IMPLEMENTATION OF GUIDANCE AND COUNSELING PROGRAMS IN STUDENT LEARNING IN ELEMENTARY SCHOOLS
Authors: Firdiana Niyang Ayu , Sefina  , Hanikah  , Fikriyah
Published: April 2024

It is very important to have a good understanding of guidance and counseling in the education of students in primary schools, especially with regard to teaching and learning activities in the classroom. The objectives of this article are to: a) Explain how the guidance and counseling program is implemented in elementary schools; b) Explain the role of teachers in implementing the guidance and counseling program; c) Explain teachers' efforts to implement student learning guidance; and d) Explain how guidance and counseling teachers communicate with parents about student learning outcomes. This research is categorized as field research, which uses a descriptive qualitative approach. Data collection methods used observation, in-depth interviews, and documentation. Data analysis uses qualitative analysis, which means that the data is described in words or sentences and then comes to a conclusion through the following steps:: a) data reduction; b) data presentation (data display), c) conclusion drawing (verification). The results of this study indicate that at SD Negeri 1 Kenanga there is already a counseling guidance program (BK) which includes student learning guidance services. The teacher as the executor of the guidance provider has provided learning guidance to students who are experiencing difficulties well.
Optimizing Treatment of Schizophrenia: Clinical and Economical Potential for Patient Switching to Long‑Acting Injectables
Authors: Zaniolo Orietta , Maffezzoli Silvia , Povero Massimiliano , Pradelli Lorenzo
Published: April 2024

INTRODUCTION: Long-acting injectable antipsychotics (LAIs), due to a lower frequency of administration, could address the well-established challenge of non-adherence to oral ones. We conducted a Network Meta-Analysis (NMA) to assess the relationship between administration frequency and effectiveness. The recent introduction of a semi-annual paliperidone palmitate formulation, along with the arrival of monthly paliperidone generics, could emphasize LAI’s advantages. Aim of this paper is to present the results derived from an updated version of our previous NMA. These results are used to fuel a budget impact model built to evaluate the economic implications of optimizing dosing intervals.METHODS: We compare the current distribution of patients among available LAI active substances and dosing frequencies with an optimized scenario. In this scenario, 20% of patients are switched to the next permitted regimen with a longer inter-dosing interval. Drug acquisition costs and relapse management costs are taken into account over a one-year simulation period; these last items are estimated by means of the event rates obtained from the updated meta-analysis. The optimized scenario incorporates the reduced cost resulting from the expiration of patents.RESULTS: Throughout the analysis, a total of 11,600 patients were able to switch from shorter to longer dosing intervals, leading to an overall optimization of quality of care. The greater expenditure incurred by the Italian National Health Service (NHS) in the acquisition of newer and longer-lasting drugs is offset by savings associated with the arrival of generics of monthly paliperidone palmitate and the shift toward less-relapsing regimens. The net impact on the NHS budget is a saving of more than 19 million Euros.CONCLUSION: This economic saving has the potential to initiate a virtuous process: it could be reinvested to fund a further shift from oral daily therapies, which are less expensive but marked by poor compliance, to LAIs. According to our simulation, nearly 40 thousand patients could undergo this transition, without additional expenses for the NHS.
Current status and perspectives of developement of vegetable crop production in the Republic of Srpska
Authors: Тоdоrоvić Vidа , Lаzić Brаnkа , Đеkić Nikоlinа
Published: April 2024

Climate changes and their impact on flora and fauna are an aggravating factor for agriculture, with a particularly negative impact on cultivated vegetables. The complexity of the problem of the adaptation of vegetable production to climate changes requires new knowledge, a holistic approach to innovative vegetable production technologies with a significant share of agro-ecological measures with respect for natural biological cycles. Climate changes will significantly affect and change vegetable production from the introduction of new species and genotypes, the adaptation of agrotechnical measures to changing dynamics of production. The rapidity and overall possibility of change depends on the current state of vegetable production, the adoption of sustainable production systems and new concepts of healthy eating. Vegetable production in the Republic of Srpska has a long tradition, where about 30-40 different types of vegetables are grown, including the less widespread ones. The characteristics of agricultural production in the Republic of Srpska are low productivity, unfavorable farm structure, insufficient and poor technological equipment, dependence on imports of mainly all inputs needed for production, as seed material, plant protection products and mineral fertilizers, equipment and agricultural machinery. Agricultural land in the Republic of Srpska occupied 1,251,691 hectares, of which 893,540 hectares are arable, of which 614,264 hectares are under arable land and gardens. Of the total arable land, about 10% is sown by vegetable species. The largest amount of production is in the open field, in gardens and fields. Vegetable production in our country has all the characteristics of production in the region of Southeast Europe, but there are also modern systems that are at the level of developed countries. The underdeveloped food industry, uncertain marketing, limited markets and small exports are the main causes of the slow development of vegetable production. In addition, small parcels, a large share of human labor, lack of specialized vegetable mechanization, slow introduction of innovations, insufficient education and knowledge of producers make vegetable production expensive and uncompetitive. The chances for improvement are the introductionof new technologies, which should be seen as tools for changes in production methods and in the application of agri-environmental measures. Changes need to be made through the farmer's production system with a balanced crop and livestock production ratio and adapted to different vegetable production methods. Part of these changes requires a fundamental change in production methods, because the future of agriculture is sustainable eco-systems that produce high-quality, safe and diverse vegetables with the rational use of natural resources and the protection of biodiversity.
Production technology of fermented meat and milk products
Authors: Danilović Bojana , Savić Dragiša
Published: April 2024

Fermented food has a significant socio-economic role in the world and in some regions it is involved in nutrition of millions of people. Special attention is attracted by the use of fermented food as traditional, but, also, as functional food with health benefits. The great number of different types of fermented food is produced worldwide depending on the region, raw materials and involved microorganisms, as well as fermentation conditions. Fermented food made from materials of animal origin is produced mainly by traditional procedure under primitive conditions and in small quantities. Cheeses and sausages belong to the fermented food of animal orogin which are the most frequently produced worldwide. In this paper, a review of fermented products of animal origin was presented, with special emphasis on production processes and parameters, as well as microorganismsnvolved in the process. This chapter presents summarized data about fermented food made of meat (sausages and prosciutto) and milk (yoghurt, kefir, cheeses and kaymak) frequently found in Balkan region, bu also in Europe.
Clothing as a sociocultural phenomenon (based on materials from modern China)
Authors: Zhang Miao
Published: April 2024

The object of the study is clothing as a sociocultural phenomenon, a product of material and spiritual cultures. The evolution of clothing is closely related to sociocultural changes in society. The subject of the study is the transformation of clothing in China under the influence of political, economic, social, and aesthetic factors after the beginning of Chinese economic reform and opening up policy. The significant changes have taken place in Chinese clothing, the main of which was the transition from a uniform style of clothing for the whole country to a variety of fashion and styles, as well as the revival of traditional clothing. The transformation in the style and production cycle of clothing in China is based on objective (technology development, market laws, global fashion trends) and subjective reasons (psychology, personal preferences and aesthetic tastes of consumers). There is always a concept behind the behavior in clothes, and the desire for beauty is the main driving force behind the development of clothing. The social psychology of imitation and difference contributes to the spread of fashion trends in clothing. The author's special contribution to the topic is a detailed examination of the impact on the transformation of clothing of political, economic, sociocultural, aesthetic factors, as well as cultural exchanges and folk traditions. The author traces the sequence of fashion trends in China in recent decades. Сontemporary changes in Chinese clothing culture are not limited to simply borrowing Western trends, but are accompanied by the emergence of postmodern ideas and the promotion of the globalization process, which leads not only to the technological and design enrichment of clothing characteristics in China, but also to the growth of cultural awareness and the revivalization of traditional and local cultures.
Membrane-bound O-acyltransferase 7 (MBOAT7) shapes lysosomal lipid homeostasis and function to control alcohol-associated liver injury
Authors: Varadharajan Venkateshwari , Ramachandiran Iyappan , Massey William J , Jain Raghav , Banerjee Rakhee , Horak Anthony J , McMullen Megan R , Huang Emily , Bellar Annette , Lorkowski Shuhui W , Gulshan Kailash , Helsley Robert N , James Isabella , Pathak Vai , Dasarathy Jaividhya , Welch Nicole , Dasarathy Srinivasan , Streem David , Reizes Ofer , Allende Daniela S , Smith Jonathan D , Simcox Judith , Nagy Laura E , Brown J Mark
Published: April 2024

Recent genome-wide association studies (GWAS) have identified a link between single-nucleotide polymorphisms (SNPs) near the MBOAT7 gene and advanced liver diseases. Specifically, the common MBOAT7 variant (rs641738) associated with reduced MBOAT7 expression is implicated in non-alcoholic fatty liver disease (NAFLD), alcohol-associated liver disease (ALD), and liver fibrosis. However, the precise mechanism underlying MBOAT7-driven liver disease progression remains elusive. Previously, we identified MBOAT7-driven acylation of lysophosphatidylinositol lipids as key mechanism suppressing the progression of NAFLD (Gwag et al., 2019). Here, we show that MBOAT7 loss of function promotes ALD via reorganization of lysosomal lipid homeostasis. Circulating levels of MBOAT7 metabolic products are significantly reduced in heavy drinkers compared to healthy controls. Hepatocyte- (Mboat7-HSKO), but not myeloid-specific (Mboat7-MSKO), deletion of Mboat7 exacerbates ethanol-induced liver injury. Lipidomic profiling reveals a reorganization of the hepatic lipidome in Mboat7-HSKO mice, characterized by increased endosomal/lysosomal lipids. Ethanol-exposed Mboat7-HSKO mice exhibit dysregulated autophagic flux and lysosomal biogenesis, associated with impaired transcription factor EB-mediated lysosomal biogenesis and autophagosome accumulation. This study provides mechanistic insights into how MBOAT7 influences ALD progression through dysregulation of lysosomal biogenesis and autophagic flux, highlighting hepatocyte-specific MBOAT7 loss as a key driver of ethanol-induced liver injury.
Antipsychotic-induced epigenomic reorganization in frontal cortex of individuals with schizophrenia
Authors: Zhu Bohan , Ainsworth Richard I , Wang Zengmiao , Liu Zhengzhi , Sierra Salvador , Deng Chengyu , Callado Luis F , Meana J Javier , Wang Wei , Lu Chang , González-Maeso Javier
Published: April 2024

Genome-wide association studies have revealed >270 loci associated with schizophrenia risk, yet these genetic factors do not seem to be sufficient to fully explain the molecular determinants behind this psychiatric condition. Epigenetic marks such as post-translational histone modifications remain largely plastic during development and adulthood, allowing a dynamic impact of environmental factors, including antipsychotic medications, on access to genes and regulatory elements. However, few studies so far have profiled cell-specific genome-wide histone modifications in postmortem brain samples from schizophrenia subjects, or the effect of antipsychotic treatment on such epigenetic marks. Here, we conducted ChIP-seq analyses focusing on histone marks indicative of active enhancers (H3K27ac) and active promoters (H3K4me3), alongside RNA-seq, using frontal cortex samples from antipsychotic-free (AF) and antipsychotic-treated (AT) individuals with schizophrenia, as well as individually matched controls (n=58). Schizophrenia subjects exhibited thousands of neuronal and non-neuronal epigenetic differences at regions that included several susceptibility genetic loci, such as NRG1, DISC1, and DRD3. By analyzing the AF and AT cohorts separately, we identified schizophrenia-associated alterations in specific transcription factors, their regulatees, and epigenomic and transcriptomic features that were reversed by antipsychotic treatment; as well as those that represented a consequence of antipsychotic medication rather than a hallmark of schizophrenia in postmortem human brain samples. Notably, we also found that the effect of age on epigenomic landscapes was more pronounced in frontal cortex of AT-schizophrenics, as compared to AF-schizophrenics and controls. Together, these data provide important evidence of epigenetic alterations in the frontal cortex of individuals with schizophrenia, and remark for the first time on the impact of age and antipsychotic treatment on chromatin organization.
CoCoNuTs: A diverse subclass of Type IV restriction systems predicted to target RNA
Authors: Bell Ryan T. , Sahakyan Harutyun , Makarova Kira S. , Wolf Yuri I. , Koonin Eugene V.
Published: April 2024

A comprehensive census of McrBC systems, among the most common forms of prokaryotic Type IV restriction systems, followed by phylogenetic analysis, reveals their enormous abundance in diverse prokaryotes and a plethora of genomic associations. We focus on a previously uncharacterized branch, which we denote CoCoNuTs (
co
iled-
co
il
nu
clease tandems) for their salient features: the presence of extensive coiled-coil structures and tandem nucleases. The CoCoNuTs alone show extraordinary variety, with 3 distinct types and multiple subtypes. All CoCoNuTs contain domains predicted to interact with translation system components, such as OB-folds resembling the SmpB protein that binds bacterial transfer-messenger RNA (tmRNA), YTH-like domains that might recognize methylated tmRNA, tRNA, or rRNA, and RNA-binding Hsp70 chaperone homologs, along with RNases, such as HEPN domains, all suggesting that the CoCoNuTs target RNA. Many CoCoNuTs might additionally target DNA, via McrC nuclease homologs. Additional restriction systems, such as Type I RM, BREX, and Druantia Type III, are frequently encoded in the same predicted superoperons. In many of these superoperons, CoCoNuTs are likely regulated by cyclic nucleotides, possibly, RNA fragments with cyclic termini, that bind associated CARF (
C
RISPR-
A
ssociated
R
ossmann
F
old) domains. We hypothesize that the CoCoNuTs, together with the ancillary restriction factors, employ an echeloned defense strategy analogous to that of Type III CRISPR-Cas systems, in which an immune response eliminating virus DNA and/or RNA is launched first, but then, if it fails, an abortive infection response leading to PCD/dormancy via host RNA cleavage takes over.
Dual targeting of histone deacetylases and MYC as potential treatment strategy for H3-K27M pediatric gliomas
Authors: Algranati Danielle , Oren Roni , Dassa Bareket , Fellus-Alyagor Liat , Plotnikov Alexander , Barr Haim , Harmelin Alon , London Nir , Ron Guy , Furth Noa , Shema Efrat
Published: April 2024

Diffuse midline gliomas (DMGs) are aggressive and fatal pediatric tumors of the central nervous system that are highly resistant to treatments. Lysine to methionine substitution of residue 27 on histone H3 (H3-K27M) is a driver mutation in DMGs, reshaping the epigenetic landscape of these cells to promote tumorigenesis. H3-K27M gliomas are characterized by deregulation of histone acetylation and methylation pathways, as well as the oncogenic MYC pathway. In search of effective treatment, we examined the therapeutic potential of dual targeting of histone deacetylases (HDACs) and MYC in these tumors. Treatment of H3-K27M patient-derived cells with Sulfopin, an inhibitor shown to block MYC-driven tumors in vivo, in combination with the HDAC inhibitor Vorinostat, resulted in substantial decrease in cell viability. Moreover, transcriptome and epigenome profiling revealed synergistic effect of this drug combination in downregulation of prominent oncogenic pathways such as mTOR. Finally, in vivo studies of patient-derived orthotopic xenograft models showed significant tumor growth reduction in mice treated with the drug combination. These results highlight the combined treatment with PIN1 and HDAC inhibitors as a promising therapeutic approach for these aggressive tumors.
Motor neurons are dispensable for the assembly of a sensorimotor circuit for gaze stabilization
Authors: Goldblatt Dena , Rosti Basak , Hamling Kyla Rose , Leary Paige , Panchal Harsh , Li Marlyn , Gelnaw Hannah , Huang Stephanie , Quainoo Cheryl , Schoppik David
Published: April 2024

Sensorimotor reflex circuits engage distinct neuronal subtypes, defined by precise connectivity, to transform sensation into compensatory behavior. Whether and how motor neuron populations specify the subtype fate and/or sensory connectivity of their pre-motor partners remains controversial. Here, we discovered that motor neurons are dispensable for proper connectivity in the vestibular reflex circuit that stabilizes gaze. We first measured activity following vestibular sensation in pre-motor projection neurons after constitutive loss of their extraocular motor neuron partners. We observed normal responses and topography indicative of unchanged functional connectivity between sensory neurons and projection neurons. Next, we show that projection neurons remain anatomically and molecularly poised to connect appropriately with their downstream partners. Lastly, we show that the transcriptional signatures that typify projection neurons develop independently of motor partners. Our findings comprehensively overturn a long-standing model: that connectivity in the circuit for gaze stabilization is retrogradely determined by motor partner-derived signals. By defining the contribution of motor neurons to specification of an archetypal sensorimotor circuit, our work speaks to comparable processes in the spinal cord and advances our understanding of principles of neural development.
Propionyl-CoA carboxylase subunit B regulates anti-tumor T cells in a pancreatic cancer mouse model
Authors: Han Han V. , Efem Richard , Rosati Barbara , Lu Kevin , Maimouni Sara , Jiang Ya-Ping , Montoya Valeria , Van Der Velden Adrianus W. M. , Zong Wei-Xing , Lin Richard Z.
Published: April 2024

Most human pancreatic ductal adenocarcinoma (PDAC) are not infiltrated with cytotoxic T cells and are highly resistant to immunotherapy. Over 90% of PDAC have oncogenic KRAS mutations, and phosphoinositide 3-kinases (PI3Ks) are direct effectors of KRAS. Our previous study demonstrated that ablation of
Pik3ca
in KPC (
Kras
G12D
;
Trp53
R172H
;
Pdx1-Cre
) pancreatic cancer cells induced host T cells to infiltrate and completely eliminate the tumors in a syngeneic orthotopic implantation mouse model. Now, we show that implantation of
Pik3ca
-/-
KPC (named αKO) cancer cells induces clonal expansion of cytotoxic T cells infiltrating the pancreatic tumors. To identify potential molecules that can regulate the activity of these anti-tumor T cells, we conducted an
in vivo
genome-wide gene-deletion screen using αKO cells implanted in the mouse pancreas. The result shows that deletion of propionyl-CoA carboxylase subunit B gene (
Pccb
) in αKO cells (named p-αKO) leads to immune evasion, tumor progression and death of host mice. Surprisingly, p-αKO tumors are still infiltrated with clonally expanded CD8
+
T cells but they are inactive against tumor cells. However, blockade of PD-L1/PD1 interaction reactivated these clonally expanded T cells infiltrating p-αKO tumors, leading to slower tumor progression and improve survival of host mice. These results indicate that
Pccb
can modulate the activity of cytotoxic T cells infiltrating some pancreatic cancers and this understanding may lead to improvement in immunotherapy for this difficult-to-treat cancer.
How we stepped up to support others
Authors: Elliott Christina , Brundell Patrick
Published: April 2024

From diagnosis and disclosure to leading change, two neurodivergent researchers recount their experiences setting up peer support networks at their universities.
Report Evaluating Safety Culture at UiT
Authors: Wolfson Deanna , Kjellman Sofia E. , Boomgaren Marc , Oppvang Janne , Bjørkeng Eva Katrin
Published: April 2024

The system of safety associated with lab and fieldwork at UiT The Arctic University of Norway’s Tromsø campus is in serious need of improvement. A volunteer working group of employees evaluated current safety conditions at NT-faculty and beyond, and found a wide range of issues with many overlapping causes. This report contains their findings related to three overarching problem areas: communication, accountability, and training; as well as a range of topical safety areas such as chemicals, fieldwork, and biohazards. The report concludes that significant reform is needed from the top and at all levels to prevent both physical injury and further damage to the working environment.
Systematic review of predictive maintenance and digital twin technologies challenges, opportunities, and best practices
Authors: Abd Wahab Nur Haninie , Hasikin Khairunnisa , Wee Lai Khin , Xia Kaijian , Bei Lulu , Huang Kai , Wu Xiang
Published: April 2024

Background

Maintaining machines effectively continues to be a challenge for industrial organisations, which frequently employ reactive or premeditated methods. Recent research has begun to shift its attention towards the application of Predictive Maintenance (PdM) and Digital Twins (DT) principles in order to improve maintenance processes. PdM technologies have the capacity to significantly improve profitability, safety, and sustainability in various industries. Significantly, precise equipment estimation, enabled by robust supervised learning techniques, is critical to the efficacy of PdM in conjunction with DT development. This study underscores the application of PdM and DT, exploring its transformative potential across domains demanding real-time monitoring. Specifically, it delves into emerging fields in healthcare, utilities (smart water management), and agriculture (smart farm), aligning with the latest research frontiers in these areas.

Methodology

Employing the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) criteria, this study highlights diverse modeling techniques shaping asset lifetime evaluation within the PdM context from 34 scholarly articles.

Results

The study revealed four important findings: various PdM and DT modelling techniques, their diverse approaches, predictive outcomes, and implementation of maintenance management. These findings align with the ongoing exploration of emerging applications in healthcare, utilities (smart water management), and agriculture (smart farm). In addition, it sheds light on the critical functions of PdM and DT, emphasising their extraordinary ability to drive revolutionary change in dynamic industrial challenges. The results highlight these methodologies’ flexibility and application across many industries, providing vital insights into their potential to revolutionise asset management and maintenance practice for real-time monitoring.

Conclusions

Therefore, this systematic review provides a current and essential resource for academics, practitioners, and policymakers to refine PdM strategies and expand the applicability of DT in diverse industrial sectors.
DenseHillNet: a lightweight CNN for accurate classification of natural images
Authors: Saqib Sheikh Muhammad , Zubair Asghar Muhammad , Iqbal Muhammad , Al-Rasheed Amal , Amir Khan Muhammad , Ghadi Yazeed , Mazhar Tehseen
Published: April 2024

The detection of natural images, such as glaciers and mountains, holds practical applications in transportation automation and outdoor activities. Convolutional neural networks (CNNs) have been widely employed for image recognition and classification tasks. While previous studies have focused on fruits, land sliding, and medical images, there is a need for further research on the detection of natural images, particularly glaciers and mountains. To address the limitations of traditional CNNs, such as vanishing gradients and the need for many layers, the proposed work introduces a novel model called DenseHillNet. The model utilizes a DenseHillNet architecture, a type of CNN with densely connected layers, to accurately classify images as glaciers or mountains. The model contributes to the development of automation technologies in transportation and outdoor activities. The dataset used in this study comprises 3,096 images of each of the “glacier” and “mountain” categories. Rigorous methodology was employed for dataset preparation and model training, ensuring the validity of the results. A comparison with a previous work revealed that the proposed DenseHillNet model, trained on both glacier and mountain images, achieved higher accuracy (86%) compared to a CNN model that only utilized glacier images (72%). Researchers and graduate students are the audience of our article.
Kernel density estimation of allele frequency including undetected alleles
Authors: Aoki Satoshi , Fukasawa Keita
Published: April 2024

Whereas undetected species contribute to estimation of species diversity, undetected alleles have not been used to estimated genetic diversity. Although random sampling guarantees unbiased estimation of allele frequency and genetic diversity measures, using undetected alleles may provide biased but more precise estimators useful for conservation. We newly devised kernel density estimation (KDE) for allele frequency including undetected alleles and tested it in estimation of allele frequency and nucleotide diversity using population generated by coalescent simulation as well as well as real population data. Contrary to expectations, nucleotide diversity estimated by KDE had worse bias and accuracy. Allele frequency estimated by KDE was also worse except when the sample size was small. These might be due to finity of population and/or the curse of dimensionality. In conclusion, KDE of allele frequency does not contribute to genetic diversity estimation.
Fumaric acid production from fermented oil palm empty fruit bunches using fungal isolate K20: a comparison between free and immobilized cells
Authors: Boondaeng Antika , Keabpimai Jureeporn , Trakunjae Chanaporn , Niyomvong Nanthavut
Published: April 2024

This study investigated the potential of using steam-exploded oil palm empty fruit bunches (EFB) as a renewable feedstock for producing fumaric acid (FA), a food additive widely used for flavor and preservation, through a separate hydrolysis and fermentation process using the fungal isolate K20. The efficiency of FA production by free and immobilized cells was compared. The maximum FA concentration (3.25 g/L), with 0.034 g/L/h productivity, was observed after incubation with the free cells for 96 h. Furthermore, the production was scaled up in a 3-L air-lift fermenter using oil palm EFB-derived glucose as the substrate. The FA concentration, yield, and productivity from 100 g/L initial oil palm EFB-derived glucose were 44 g/L, 0.39 g/g, and 0.41 g/L/h, respectively. The potential for scaling up the fermentation process indicates favorable results, which could have significant implications for industrial applications.
Reimagining urban habitats to benefit people and nature
Authors: Threlfall Caragh G. , Farrell Claire , Lequerica Tamara Manuel E. , Hochuli Dieter F.
Published: April 2024

Urbanisation is a leading cause of global biodiversity loss, imposing the most rapid and ecologically damaging impacts of any human driven land-use change. Despite the trend of biodiversity decline, urban nature provides many health, wellbeing and workplace productivity benefits to city dwellers. Hence, there is an urgent need to return nature to cities not only to conserve biodiversity, but also to maintain human experiences of nature. To meet this challenge, there are currently significant global attempts to re-green cities to improve environmental condition, including restoring habitat for biodiversity. However, many barriers to widespread implementation still exist, including competition for limited space, a lack of technical capacity, and a disengaged community. New approaches to urban restoration are urgently needed that suit the small fragments of space available, and that can deliver multiple benefits not only to conserve urban biodiversity but also to reconnect people with nature. To overcome these challenges, an ‘ecology with cities’ perspective, combining horticultural, ecological and social approaches to urban habitat management and restoration, is needed. Significant opportunities exist for urban ecologists and zoologists to engage with practitioners and the community to co-develop and implement approaches to successfully achieve the aim of creating biodiverse urban environments.
Tourism Policies and Performance of Nigerian Heritage Sites
Authors: Adetola Bukola Omotomilola , Akinola Olagoke, Oluseyi , Aina Ayeni, Dorcas
Published: April 2024

Background: Tourism policies have a significant impact on the preservation, promotion, and overall performance of heritage sites for both domestic and international tourists. This review provides insights into the entities responsible for tourism policy formulation, the policy objective, and how the stated objectives are being realized while shedding light on their implications for sustainability, economic growth, and cultural preservation. The main objective of Nigeria's tourism policy is to protect and improve the nation's natural and cultural heritage. Many activities aimed at encouraging tourism development include planning, provision of infrastructure, taxation regime that supports tourism growth, education and training for tourism employees, marketing, legislation and regulation in order to protect and preserve tourist facilities as well as the country's natural resources, historical and cultural assets. The critical concern is the enforcement and effectiveness of regulations, specifically the probability of a policy successfully promoting sustainable and viable tourism development and management.
Aims: This paper therefore investigates the influence of tourism policies on the performance of heritage sites in Nigeria and the need for policy review for improved performance of Nigerian heritage sites.
Methodology: The qualitative research paradigm emerged from a comprehensive review of tourism policy literature in Nigeria. The examination of documents and research projects focused on various aspects of tourism policy planning and governance, policy formulation, policy objectives, and their implications for sustainability, economic growth, and natural/cultural heritage preservation. In the data analysis, the comprehensive review were presented with an interpretative narrative.
Results:  The findings revealed that Nigeria's tourism policy seeks to empower the tourism industry and position it as a key driver of economic growth, job creation, and cultural heritage preservation.  However, the policy's ability to achieve its goals optimally has been significantly hampered by lack of relevant mechanisms to measure overall levels of compliance, overreliance on petroleum resources, poor planning, and inadequate participation of relevant stakeholders in policy formulation, implementation, and monitoring.
Conclusion: Despite policies and frameworks, Nigeria's tourism sector, including cultural heritage sites, has failed to reach its goals, losing the economy numerous potential benefits. In order to address the current circumstances and ensure successful heritage site design, development, and management in Nigeria, there is an urgent need for revision of the country’s tourism policy objectives and adequate enforcement measures implemented to adapt to changing circumstances and priorities.
A Case Study on Brand Personality and Consumer Loyalty in India's Booming Dairy Industry
Authors: N Venkatesa Palanichamy , M Kalpana , P Sivapriya , M Santhosh Kumar
Published: April 2024

India's dairy industry is witnessing significant growth, driven by factors such as rising disposable incomes, evolving consumer preferences, and the emergence of foreign brands. As the market expands, establishing a strong brand image becomes essential. This research focuses on assessing the brand personality of AMUL, a prominent player in the Indian dairy market, and its influence on consumer loyalty. Utilizing a structured questionnaire administered to respondents in Coimbatore District, this study measures consumers' attitudes towards AMUL, its brand personality. Through exploratory factor analysis, four key dimensions of AMUL's brand personality viz., activity, simplicity, responsibility and emotionality are identified. The findings reveal a positive correlation between brand personality and consumer attitude, indicating the importance of personality in brand perception. The study underscores the importance of aligning branding efforts with consumer preferences and suggests strategies for AMUL to enhance its brand image and brand personality. By leveraging these insights, AMUL and other dairy firms can effectively engage consumers, strengthen brand equity, and sustain market competitiveness in India's dynamic dairy landscape.
Hurried Child Syndrome: Are we Creating an Advanced Generation or Destroying it?
Authors: Upashe Shivaleela P , Shil Rahul , Das Shreyasee
Published: April 2024

Parents who overschedule their children, put a lot of pressure on them to succeed academically, and want them to act and behave like tiny adults beyond their mental, social, or emotional capabilities are considered to be suffering from the disease known as "hurried child syndrome." Due to this, there has been a 50% rise in childhood obesity, a tripling in suicide and murder rates, 15-20% of young children are "flunking" kindergarden and millions of children are medicated in order to make them more 'controllable' at home and in school over the last 20 years. notably typical symptoms are headaches, diarrhoea, hyperactivity, cramping in the muscles, insomnia, digestive issues, and bedwetting. Drug and alcohol dependency is currently the most prevalent cause of death among adolescents, moreover, suicide incidents are also rising. About 70% of teenage girls will not be virgins by the time they reach adulthood, and 40% of those who are sexually active will become pregnant. The most significant aspect is to pay close attention to the adolescents and attempt to comprehend their unique needs and abilities. It's wonderful if a child loves education and engaging in extracurricular activities, but if they are having difficulty or are resistant, it's best to step back and give them space to learn as much as they can at their own pace.