bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries
Authors: Alhussein Abdullah , Alsahafi Rashed , Balhaddad Abdulrahman A. , Mokeem Lamia , Schneider Abraham , Jabra-Rizk Mary-Ann , Masri Radi , Hack Gary D. , Oates Thomas W. , Sun Jirun , Weir Michael D. , Xu Hockin H. K.
Published: August 2023

Objectives: Composites are commonly used for tooth restorations, but recurrent caries often lead to restoration failures due to polymerization shrinkage-stress-induced marginal leakage. The aims of this research were to: (1) develop novel low-shrinkage-stress (L.S.S.) nanocomposites containing dimethylaminododecyl methacrylate (DMADDM) with nanoparticles of calcium fluoride (nCaF2) or amorphous calcium phosphate (NACP) for remineralization; (2) investigate antibacterial and cytocompatibility properties. Methods: Nanocomposites were made by mixing triethylene glycol divinylbenzyl ether with urethane dimethacrylate containing 3% DMADDM, 20% nCaF2, and 20% NACP. Flexural strength, elastic modulus, antibacterial properties against Streptococcus mutans biofilms, and cytotoxicity against human gingival fibroblasts and dental pulp stem cells were tested. Results: Nanocomposites with DMADDM and nCaF2 or NACP had flexural strengths matching commercial composite control without bioactivity. The new nanocomposite provided potent antibacterial properties, reducing biofilm CFU by 6 logs, and reducing lactic acid synthesis and metabolic function of biofilms by 90%, compared to controls (p < 0.05). The new nanocomposites produced excellent cell viability matching commercial control (p > 0.05). Conclusions: Bioactive L.S.S. antibacterial nanocomposites with nCaF2 and NACP had excellent bioactivity without compromising mechanical and cytocompatible properties. The new nanocomposites are promising for a wide range of dental restorations by improving marginal integrity by reducing shrinkage stress, defending tooth structures, and minimizing cariogenic biofilms.
MALDI-TOF MS Analysis of Serum Peptidome Patterns in Cervical Cancer
Authors: Rungkamoltip Phetploy , Roytrakul Sittiruk , Navakanitworakul Raphatphorn
Published: August 2023

Background: Cervical cancer is the fourth most common cancer among females worldwide. Identifying peptide patterns discriminating healthy individuals from those with diseases has gained interest in the early detection of cancers. Our study aimed to determine signature peptide patterns for cervical cancer screening. Methods: Our study focused on the serum peptidome analysis of 83 healthy women and 139 patients with cervical cancer. All spectra derived from matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were analyzed using FlexAnalysis 3.0 and ClinProTools 2.2 software. Results: In the mass range of 1000–10,000 Da, the total average spectra were represented as the signature pattern. Principal component analysis showed that all the groups were separately distributed. Furthermore, the peaks at m/z 1466.91, 1898.01, 3159.09, and 4299.40 significantly differed among the investigated groups (Wilcoxon/Kruskal–Wallis test and ANOVA, p < 0.001). Conclusions: Laboratory-based rapid mass spectrometry showed that serum peptidome patterns could serve as diagnostic tools for diagnosing cervical cancer; however, verification through larger cohorts and association with clinical data are required, and the use of externally validated samples, such as patients with other types of cancers, should be investigated to validate the specific peptide patterns.
Does β-Hydroxy-β-Methylbutyrate Have Any Potential to Support the Treatment of Duchenne Muscular Dystrophy in Humans and Animals?
Authors: Gorji Abdolvahab Ebrahimpour , Ostaszewski Piotr , Urbańska Kaja , Sadkowski Tomasz
Published: August 2023

Skeletal muscle is the protein reservoir of our body and an important regulator of glucose and lipid homeostasis. The dystrophin gene is the largest gene and has a key role in skeletal muscle construction and function. Mutations in the dystrophin gene cause Duchenne and Becker muscular dystrophy in humans, mice, dogs, and cats. Duchenne muscular dystrophy (DMD) is an X-linked neuromuscular condition causing progressive muscle weakness and premature death. β-hydroxy β-methylbutyrate (HMB) prevents deleterious muscle responses under pathological conditions, including tumor and chronic steroid therapy-related muscle losses. The use of HMB as a dietary supplement allows for increasing lean weight gain; has a positive immunostimulatory effect; is associated with decreased mortality; and attenuates sarcopenia in elderly animals and individuals. This study aimed to identify some genes, metabolic pathways, and biological processes which are common for DMD and HMB based on existing literature and then discuss the consequences of that interaction.
Current Advances in Mitochondrial Targeted Interventions in Alzheimer’s Disease
Authors: Sousa Tiago , Moreira Paula I. , Cardoso Susana
Published: August 2023

Alzheimer’s disease is the most prevalent neurodegenerative disorder and affects the lives not only of those who are diagnosed but also of their caregivers. Despite the enormous social, economic and political burden, AD remains a disease without an effective treatment and with several failed attempts to modify the disease course. The fact that AD clinical diagnosis is most often performed at a stage at which the underlying pathological events are in an advanced and conceivably irremediable state strongly hampers treatment attempts. This raises the awareness of the need to identify and characterize the early brain changes in AD, in order to identify possible novel therapeutic targets to circumvent AD’s cascade of events. One of the most auspicious targets is mitochondria, powerful organelles found in nearly all cells of the body. A vast body of literature has shown that mitochondria from AD patients and model organisms of the disease differ from their non-AD counterparts. In view of this evidence, preserving and/or restoring mitochondria’s health and function can represent the primary means to achieve advances to tackle AD. In this review, we will briefly assess and summarize the previous and latest evidence of mitochondria dysfunction in AD. A particular focus will be given to the recent updates and advances in the strategy options aimed to target faulty mitochondria in AD.
Integrins and Actions of Androgen in Breast Cancer
Authors: Tsai Chung-Che , Yang Yu-Chen S. H. , Chen Yi-Fong , Huang Lin-Yi , Yang Yung-Ning , Lee Sheng-Yang , Wang Wen-Long , Lee Hsin-Lun , Whang-Peng Jacqueline , Lin Hung-Yun , Wang Kuan
Published: August 2023

Androgen has been shown to regulate male physiological activities and cancer proliferation. It is used to antagonize estrogen-induced proliferative effects in breast cancer cells. However, evidence indicates that androgen can stimulate cancer cell growth in estrogen receptor (ER)-positive and ER-negative breast cancer cells via different types of receptors and different mechanisms. Androgen-induced cancer growth and metastasis link with different types of integrins. Integrin αvβ3 is predominantly expressed and activated in cancer cells and rapidly dividing endothelial cells. Programmed death-ligand 1 (PD-L1) also plays a vital role in cancer growth. The part of integrins in action with androgen in cancer cells is not fully mechanically understood. To clarify the interactions between androgen and integrin αvβ3, we carried out molecular modeling to explain the potential interactions of androgen with integrin αvβ3. The androgen-regulated mechanisms on PD-L1 and its effects were also addressed.
Liver Injury and Regeneration: Current Understanding, New Approaches, and Future Perspectives
Authors: Hora Shainan , Wuestefeld Torsten
Published: August 2023

The liver is a complex organ with the ability to regenerate itself in response to injury. However, several factors can contribute to liver damage beyond repair. Liver injury can be caused by viral infections, alcoholic liver disease, non-alcoholic steatohepatitis, and drug-induced liver injury. Understanding the cellular and molecular mechanisms involved in liver injury and regeneration is critical to developing effective therapies for liver diseases. Liver regeneration is a complex process that involves the interplay of various signaling pathways, cell types, and extracellular matrix components. The activation of quiescent hepatocytes that proliferate and restore the liver mass by upregulating genes involved in cell-cycle progression, DNA repair, and mitochondrial function; the proliferation and differentiation of progenitor cells, also known as oval cells, into hepatocytes that contribute to liver regeneration; and the recruitment of immune cells to release cytokines and angiogenic factors that promote or inhibit cell proliferation are some examples of the regenerative processes. Recent advances in the fields of gene editing, tissue engineering, stem cell differentiation, small interfering RNA-based therapies, and single-cell transcriptomics have paved a roadmap for future research into liver regeneration as well as for the identification of previously unknown cell types and gene expression patterns. In summary, liver injury and regeneration is a complex and dynamic process. A better understanding of the cellular and molecular mechanisms driving this phenomenon could lead to the development of new therapies for liver diseases and improve patient outcomes.
Manufacturing and Properties of Various Ceramic Embedded Composite Fabrics for Protective Clothing in Gas and Oil Industries Part I: Anti-Static and UV Protection with Thermal Radiation
Authors: Kim Hyunah
Published: August 2023

Protective clothing in gas and oil industries requires high-performance characteristics, with superior anti-static and ultraviolet (UV) protection and good thermal wear comfort in cold weather regions. This study examined the manufacturing and properties of various ceramic-embedded composite fabrics made from a new scheme (not a coating method) for protective clothing in the gas and oil industries. Therefore, sheath–core yarn specimens embedded with various ceramics, such as aluminum oxide (Al2O3)–graphite, zinc oxide–zirconium (ZnO–ZrC), and zinc oxide–antimony tin oxide (ZnO–ATO) were produced using a bi-component melt spinning machine, which is a novel method that was not tried before. Fabric specimens were also made from these ceramic-embedded sheath–core yarn specimens. UV-protection and anti-static properties of the ceramic-embedded composite specimen were compared with the thermal radiation and far-infrared (FIR) characteristics. The UV-protection factor (UPF) was measured according to the AS/NZ 4399 (1996) standard. ATLAS measuring equipment was used to analyze five duplicate specimens (4 × 8 cm). An anti-static assessment was also conducted using the JIS L 1094 standard method. A light heat emission apparatus was used to assess thermal radiation. A 10 × 10 cm specimen was prepared, and five duplicate assessments were conducted. Statistical analysis (F-test) was performed to verify the statistical significance of the experimental data with a 99% confidence limit. The ZnO–ATO-embedded composite fabric exhibited greater UV protection than the Al2O3–graphite-embedded and regular (control) specimen, indicating the excellent UV-protection property of the ZnO. In addition, the ZnO–ATO-embedded composite specimen exhibited excellent anti-static properties with lower rub-static voltage than the control fabric, which was attributed to the better electrical conductivity of ATO particles. In particular, the ZnO–ZrC-embedded composite specimen showed superior thermal radiation with excellent UPF and relatively good anti-static characteristics. Based on the high-performance characteristics of protective clothing worn in gas and oil industries, ZnO–ATO-embedded composite fabric has practical use for fabricating workwear protective clothing. In addition, considering protective clothing suitable for cold weather, ZnO–ZrC-embedded composite fabric is useful for protective clothing in cold weather regions.
Properties of Blue Phosphorene Nanoribbon-P3HT Polymer Heterostructures: DFT First Principles Calculations
Authors: Turiján-Clara Benita , Correa Julián D. , Mora-Ramos Miguel E. , Duque Carlos A.
Published: August 2023

Recently, 2D phosphorus allotropes have arisen as possible candidates for technological applications among the family of the so-called Xene layered materials. In particular, the energy band structure of blue phosphorene (BP) exhibits a medium-size semiconductor gap that tends to widen in the case of using this material in the form of ribbons. BP nanoribbons have attracted recent interest for their implication in the improvement in efficiency of novel solar cells. On the other hand, compound poly (3-hexylthiophene) (P3HT) is used as the semiconducting core of organic field effect transistors owing to such useful features as high carrier mobility. Here, we theoretically investigate the electronic properties of a heterostructure combination of BP—in the form of nanoribbons—with a P3HT polymer chain on top in order to identify the features of band alignment. The work is performed using first principles calculations via DFT, employing different exchange correlation approaches for comparison: PBE, HSE06 and DFT-1/2. It is found that, under DFT-1/2, such a heterostructure has a type-II band alignment.
The Role of Islamic Beliefs in Facilitating Acceptance of Cancer Diagnosis
Authors: Benidir Amina , Levert Marie-Josée , Bilodeau Karine
Published: August 2023

Although survival rates for patients with cancer have increased, this disease continues to affect couples significantly. Religion and culture seem to be part of the therapeutic process for people with cancer. Despite the abundance of the Arab Muslim community in Western countries, there is a lack of documented data on Arab Muslim couples experiencing cancer. A simple exploratory qualitative study was conducted through semi-structured interviews on six married couples (n = 12) identifying with the Arab Muslim culture and being affected by cancer. An iterative data analysis was performed. Results were reported under the following themes: accepting illness through coping strategies provided by Muslim religious beliefs and practices, experiencing problems with the expression of needs and feelings within the couple, experiencing closeness within the family, and experiencing illness in the hospital setting as Muslims. Our results show that Islamic beliefs can facilitate acceptance of a cancer diagnosis. It is also noted that religion seems to unite spouses in supporting each other and maintaining hope in a difficult context. Communication issues may persist between a couple due to stressors related to cancer. The results of this study could raise awareness about the importance of exploring religious and spiritual beliefs when supporting couples affected by cancer.
Disturbance Observer-Enhanced Adaptive Fault-Tolerant Control of a Quadrotor UAV against Actuator Faults and Disturbances
Authors: Hu Xinyue , Wang Ban , Shen Yanyan , Fu Yifang , Li Ni
Published: August 2023

For a quadrotor unmanned aerial vehicle (UAV), this paper proposes an adaptive sliding mode control (SMC) strategy enhanced with a disturbance observer to attain precise trajectory and attitude tracking performance while compensating for the detrimental impacts of actuator faults and disturbances. First, an adaptive SMC strategy that utilizes an integral sliding surface is presented to enhance the fault-tolerance capabilities of the studied quadrotor UAV against actuator faults. In addition, a disturbance observer is further created to compensate for the disturbances. By integrating the proposed adaptive SMC strategy with the designed disturbance observer, both actuator faults and disturbances can be effectively accommodated. It was theoretically demonstrated that the system is stable while using the proposed adaptive fault-tolerant control strategy. The effectiveness and benefits of the proposed strategy is verified with comparative simulation results under different faulty scenarios.
Interpretation of Fluoride Groundwater Contamination in Tamnar Area, Raigarh, Chhattisgarh, India
Authors: Beg Mirza Kaleem , Kumar Navneet , Srivastava S. K. , Carranza E. J. M.
Published: August 2023

A high concentration of fluoride (F−) in drinking water is harmful and is a serious concern worldwide due to its toxicity and accumulation in the human body. There are various sources of fluoride (F−) and divergent pathways to enter into groundwater sources. High F− incidence in groundwater was reported in Raigarh district of Central India in a sedimentary (Gondwana) aquifer system. The present study investigates the hydrogeochemistry of groundwater in the Tamnar area of Raigarh district to understand the plausible cause(s) of high F− concentration, especially the source(s) and underlying geochemical processes. Groundwater samples, representing pre-monsoon (N = 83), monsoon (N = 20), and post-monsoon (N = 81) seasons, and rock samples (N = 4) were collected and analyzed. The study revealed that (i) groundwater with high F− concentration occurs in the Barakar Formation, which has a litho-assemblage of feldspathic sandstones, shales, and coal, (ii) high F− concentration is mainly associated with Na-Ca-HCO3, Na-Ca-Mg-HCO3, and Na-Mg-Ca-HCO3 types of groundwater, (iii) the F− concentration increases as the ratio of Na+ and Ca2+ increases (Na+: Ca2+, concentration in meq/l), (iv) F− has significant positive correlation with Na+ and SiO2, and significant negative correlation with Ca2+, Mg2+, HCO3−, and TH, and (v) high F− concentration in groundwater is found in deeper wells. Micas and clay minerals, occurring in the feldspathic sandstones and intercalated shale/clay/coal beds, possibly form an additional source for releasing F− in groundwater. Feldspar dissolution coupled with anion (OH− or F−) and cation (Ca2+ for Na+) exchange are probably the dominant geochemical processes taking place in the study area. The higher residence time and temperature of groundwater in deeper aquifers also play a role in enhancing the dissolution of fluorine-bearing minerals. Systematic hydrogeochemical investigations are recommended in the surrounding area having a similar geologic setting in view of the potential health risk to a large population.
Exploring Social Justice Education as a Responsive Middle Grades Pedagogy to Promote Justice-Oriented Citizenship
Authors: DeMink-Carthew Jessica , DeMink Jeremy W. , Smith Kristie W.
Published: August 2023

Middle grades students across the U.S. are learning about citizenship both explicitly and overtly through the hidden curriculum, which, in the majority of schools, promotes passive compliant citizenship. Culturally and developmentally responsive teaching in the middle grades, however, necessitates active authentic learning that engages the emerging criticality and sense of justice of young adolescents. For this reason, social justice education (SJE) is an especially promising responsive pedagogy for the middle grades. This study investigates the impact of a social justice project designed to promote justice-oriented views of citizenship on middle grades student perceptions of “good citizenship”. Findings indicate shifts in student perceptions of good citizenship toward more participatory and justice-oriented views of citizenship as well as increased critical consciousness. In addition, the findings indicate two new themes in student perceptions: (a) good citizens work together and (b) good citizens sometimes have to defy norms for justice. We close with a critical analysis of the findings and implications for responsive justice-oriented teaching and scholarship.
ANN-Based Reliability Enhancement of SMPS Aluminum Electrolytic Capacitors in Cold Environments
Authors: Jeong Sunwoo , Kareem Akeem Bayo , Song Sungwook , Hur Jang-Wook
Published: August 2023

Due to their substantial energy density and economical pricing, switching-mode power supplies (SMPSs) often utilize electrolytic capacitors. However, their ability to function at low temperatures is essential for dependable operation in several sectors, including telecommunications, automotive, and aerospace. This study includes an experimental evaluation of how well standard SMPS electrolytic capacitors operate at low temperatures. This paper investigates the suitability of standard electrolytic capacitors used in switched-mode power supplies (SMPSs) for low-temperature applications. The experimental evaluation exposed the capacitors to temperatures ranging from −5 °C to −40 °C, assessing capacitance (Cp), impedance (Z), dissipation factor (DF), and equivalent series resistance (ESR) at each temperature. The capacitor’s time-domain electrical signals were analyzed using the Pearson correlation coefficient to extract discriminative features. These features were input into an artificial neural network (ANN) for training and testing. The results indicated a significant impact of low temperatures on capacitor performance. Capacitance decreased with lower temperatures, while the ESR and leakage current increased, affecting stability and efficiency. Impedance was a valuable diagnostic tool for identifying potential capacitor failure, showing a 98.44% accuracy drop at −5 °C and 88.75% at the peak temperature, indicating proximity to the manufacturer’s specified limit. The study suggests further research and development to improve the performance of electrolytic capacitors in SMPS systems under cold conditions, aiming to boost efficiency and reliability.
Demagnetization Modeling and Analysis for a Six-Phase Surface-Mounted Field-Modulated Permanent-Magnet Machine Based on Equivalent Magnetic Network
Authors: Li Xianglin , Tan Yingjie , Yan Bo , Zhao Yujian , Wang Hao
Published: August 2023

Based on the magnetic gear effect, the field-modulated permanent-magnet machine (FMPMM) can realize the unequal pole design of the rotor PM field and the stator armature magnetic field. With the advantages of high torque density and high efficiency, the FMPMM has been widely studied in low-speed direct-drive applications. As a kind of machine excited by PMs, the performance of the FMPMM was affected by the demagnetization state. However, the method for establishing the FMPMM demagnetization model based on a finite element analysis (FEA) presented some problems, including tedious repeated modeling work and long calculation time-consuming under fine subdivision. Therefore, in this paper, a six-phase surface-mounted FMPMM was taken as an example, and an equivalent magnetic network (EMN) model was proposed for evaluating the machine performance under demagnetization. In order to realize the rapid establishing EMN models under diverse demagnetization types, the variable coercivity of PM was introduced. Furthermore, for the purpose of improving the calculation accuracy and shortening the calculation time, the least square method was used in fitting and analyzing the discrete results. Then, in order to verify the validity of the proposed EMN model, a prototype was fabricated and a testing platform was built. The air-gap flux density and the no-load back EMF obtained by the FEA, the proposed EMN model, and the experimental testing were compared. The results showed that the proposed EMN model can realize the rapid modeling and accurate analysis of the six-phase surface-mounted FMPMM under diverse demagnetization types.
A Review of Current and Emerging Production Technologies for Biomass-Derived Sustainable Aviation Fuels
Authors: Peters Morenike Ajike , Alves Carine Tondo , Onwudili Jude Azubuike
Published: August 2023

The aviation industry is a significant contributor to global carbon dioxide emissions, with over 920 million tonnes per year, and there is a growing need to reduce its environmental impact. The production of biojet fuel from renewable biomass feedstocks presents a promising solution to address this challenge, with the potential to reduce greenhouse gas emissions and dependence on fossil fuels in the aviation sector. This review provides an in-depth discussion of current and emerging biojet fuel conversion technologies, their feasibility, and their sustainability, focusing on the promising conversion pathways: lipids-to-jet, sugar-to-jet, gas-to-jet, alcohol-to-jet, and whole biomass-to-jet. Each technology is discussed in terms of its associated feedstocks, important chemistries, and processing steps, with focus on recent innovations to improve yields of biojet product at the required specifications. In addition, the emerging power-to-liquid technology is briefly introduced. With the integrated biorefinery approach, consideration is given to biomass pretreatment to obtain specific feedstocks for the specific technology to obtain the final product, with the embedded environmental sustainability requirements. In addition, the review highlights the challenges associated with the biojet production technologies, with embedded suggestions of future research directions to advance the development of this important and fast-growing sustainable fuel industry.
Building Occupants, Their Behavior and the Resulting Impact on Energy Use in Campus Buildings: A Literature Review with Focus on Smart Building Systems
Authors: Bäcklund Katarina , Molinari Marco , Lundqvist Per , Palm Björn
Published: August 2023

In the light of global climate change and the current energy crisis, it is crucial to target sustainable energy use in all sectors. Buildings still remain one of the most energy-demanding sectors. Campus buildings and higher educational buildings are important to target due to their high and increasing energy demand. This building segment also represents a research gap, as mostly office or domestic buildings have been studied previously. In the quest for thermal comfort, a key stakeholder in building energy demand is the building occupant. It is therefore crucial to promote energy-aware behaviors. The building systems are another key factor to consider. As conventional building systems are replaced with smart building systems, the entire scenario is redrawn for how building occupants interact with the building and its systems. This study argues that behavior is evolving with the smartness of building systems. By means of a semi-systematic literature review, this study presents key findings from peer-reviewed research that deal with building occupant behavior, building systems and energy use in campus buildings. The literature review was an iterative process based on six predefined research questions. Two key results are presented: a graph of reported energy-saving potentials and a conceptual framework to evaluate building occupants impact on building energy use. Furthermore, based on the identified research gaps in the selected literature, areas for future research are proposed.
Impaired Soil Health in Agricultural Areas Close to Fe-Ni Mines on Euboea Island, Greece, Caused by Increased Concentrations of Potentially Toxic Elements, and the Associated Impacts on Human Health
Authors: Massas Ioannis , Kairis Orestis , Gasparatos Dionisios , Ioannou Dafni , Vatougios Dimitrios , Zafeiriou Ioannis
Published: August 2023

Agricultural soils close to mining establishments may suffer from airborne pollution, due to excavation and transportation activities. To assess the impact of soil pollution from potentially toxic elements (PTEs) on soil and human health in agricultural areas close to Fe-Ni mines, 36 composite topsoil samples were collected from central Euboea Island, Greece. The soils were analyzed for their physicochemical properties and for total and bioavailable Ni, Cr, Co, Mn, Fe, Pb, Cu, and Zn concentrations; the BCR sequential extraction protocol was additionally applied to all samples. Soil enrichment caused by the metals and the implications of soil degradation on the ecosystem were evaluated using the calculation of single pollution indices (PI) and the potential ecological risk index (RI), respectively. The hazard index (HI) for non-carcinogenic metals and life cancer risk (LCR) for carcinogenic metals were used to appraise the human health risks. Extremely high, very high, and considerably high total concentrations of Ni, Cr, and Mn, respectively, were determined. Though most of the total amounts of metals in soil samples were found to be related to the residual fraction, the considerable portion extracted in the first two steps of the BCR process can be regarded as able to introduce toxicity issues in the local biota. High PI values of Cr, Co, and especially Ni point to severely polluted soils, and the mean RI values indicate a considerable risk for biota. HI values > 1 show increased possibilities for non-carcinogenic health issues in children, whereas the LCR values of Ni were above the critical limit, 1 × 10−4, for both children and adults.
Profiling Genetic Breeding Progress in Bagrid Catfishes
Authors: Huang Lishi , Zhang Guosong , Zhang Yupeng , Li Xinlan , Luo Zhong , Liu Wenyu , Luo Fu , Liu Haifeng , Yin Shaowu , Jiang Jun , Liang Xia , Cao Quanquan
Published: August 2023

The Bagridae fish family holds significant economic value and enjoys a high demand in the commercial market due to its desirable characteristics, such as delicious meat, fewer muscle spines, and a pleasing flavor. In recent years, the Bagridae has experienced rapid development in the seed industry in response to industry demands. Researchers have conducted extensive studies on genetic breeding in the Bagridae, employing a comprehensive breeding approach that combines conventional breeding techniques with innovative new technologies and cooperative efforts. In this review, we present an overview of the market situation and discuss various breeding processes applied to the Bagridae, including selective breeding, genetic engineering breeding, cell engineering breeding, molecular marker-assisted breeding, and hybrid breeding. Additionally, we focus on introducing the cultivation methods for two new Bagridae varieties. Furthermore, we explore and summarize the breeding conditions, genetic background, heterosis, comprehensive breeding technology, stress resistance, and metabolic nutrition of hybrid Bagridae catfishes. Overall, noticeable heterosis has been observed in the breeding of the hybrid yellow catfish “Huangyou-1”, which exhibits promising market prospects and economic benefits for aquaculture. Our study aims to underscore the importance of harnessing the heterosis of catfish, particularly the Pelteobagrus and Bagridae species. Consequently, this review provides an update on the current available information regarding the seed industry of the Bagridae fish family.
Some Like It Hot: Investigating Thermoregulatory Behavior of Carcharhinid Sharks in a Natural Environment with Artificially Elevated Temperatures
Authors: Barash Adi , Scheinin Aviad , Bigal Eyal , Zemah Shamir Ziv , Martinez Stephane , Davidi Aileen , Fadida Yotam , Pickholtz Renanel , Tchernov Dan
Published: August 2023

Global warming raises seawater temperatures and creates changes which have been found to affect the movement of large migrating marine species. Understanding the thermal niches of marine species could prove essential to anticipate how the future climate will alter migrations, and how conservation efforts will have to change accordingly. Orot Rabin power station in Hadera, Israel uses seawater to cool its turbine and releases the warm water back into the Mediterranean Sea. As a result, a marine area with artificially elevated temperatures is created around the effluent. Every winter in the past two decades, this area attracts sharks of two species, Carcharhinus obscurus and Carcharhinus plumbeus, presumably to spend the cold months at a higher temperature. This study concentrated on this point of artificial heat dissipation, which maintains a wide gradient of surface temperatures and allowed us to examine the temperature preferences of these species when given a larger range than what is naturally found in the sea. Between 2016 and 2018, 16 sharks were tagged with acoustic tags, 3 of which had temperature sensors, and 2 were additionally tagged with pop-up archival tags also logging temperature data. Results show that the sharks stayed in the elevated temperature, while the ambient sea was cold during the winter, spending several months in the heated area. Both species displayed a similar preferred range, spending 90 percent of their time at a temperature between 21.8 °C and 26.1 °C while the surrounding sea was 15.5–25.5 °C. Considering this chosen thermal niche and the rise in water temperature, it appears that for the past 40 years, the Eastern shores of the Mediterranean have become more suitable for these species, especially during transitional seasons. The question that arises, however, is whether these shark populations will benefit from the expanding range of preferable temperatures, or whether their proximity to shorelines will put them at greater risk in terms of human activities such as fishing and pollution.
Characterization of Proliferation Medium and Its Effect on Differentiation of Muscle Satellite Cells from Larimichthys crocea in Cultured Fish Meat Production
Authors: Zhang Shengliang , Lou Hanghang , Lu Hongyun , Xu Enbo , Liu Donghong , Chen Qihe
Published: August 2023

To find a suitable medium for muscle satellite cells of Larimichthys crocea, herein, the effect of different basal media and coating materials on the proliferation of piscine satellite cells (PSCs) was explored. Firstly, two basal media, namely F10 and DMEM/F12, were selected as experimental materials, and high-sugar DMEM was the main basal culture medium used with fish muscle cells as a control. The results showed that the PSCs proliferated better in F10 than in DMEM/F12 or DMEM. Secondly, the effects of rat tail collagen, polylysine and matrix coatings, as compared with no coating, on the proliferation and later differentiation of PSCs were also investigated. Our results indicated that there was no significant difference between coating and no coating on the proliferation of PSCs in the F10-based medium. Meanwhile, it was found that the myotubes were washed out, and only those under matrix-coated conditions remained intact in the process of differentiation. The results also suggested that PSCs could still differentiate into myotubes without their stemness being affected after proliferation in the F10-based medium. Hence, this study identified an efficient proliferation medium based on F10 basal medium that could shorten the culture time and maintain the stemness of PSCs, thus providing a basis for large-scale cell expansion and cell-culture-based meat production in the future.
Evaluating Factors Explaining U.S. Consumers’ Behavioral Intentions toward Irradiated Ground Beef
Authors: Parrella Jean A. , Leggette Holli R. , Lu Peng , Wingenbach Gary , Baker Matt , Murano Elsa
Published: August 2023

Although food irradiation is deemed safe and endorsed by health-related organizations worldwide, consumers are reluctant to accept the technology. Yet, consumer acceptance is critical as food irradiation has significant potential for increasing the safety and availability of food globally. To communicate about food irradiation, science communicators should understand the psychology behind consumers’ decision making related to irradiated foods. Using empirical research, we developed a theoretical model and used structural equation modeling to determine how nine variables affect consumers’ behavioral intentions toward irradiated ground beef. We purchased a national quota sample from Qualtrics and surveyed N = 1102 U.S. consumers. The model explained 60.3% of the variance in consumers’ attitudes toward food irradiation and 55.4% of their behavioral intentions toward irradiated ground beef. Attitude had the largest positive, total effect on consumers’ behavioral intentions, which was followed by subjective social norm and perceived benefit. Perceived risk had the largest negative, total effect on behavioral intentions. Attitude mediated the effect of subjective social norm, perceived benefit, perceived risk, objective knowledge, and food technology neophobia. Environmental concern and health consciousness did not significantly affect behavioral intention. Science communicators should develop messaging strategies that seek to improve consumer acceptance with these factors in mind.
Rapid and Non-Invasive Assessment of Texture Profile Analysis of Common Carp (Cyprinus carpio L.) Using Hyperspectral Imaging and Machine Learning
Authors: Cao Yi-Ming , Zhang Yan , Yu Shuang-Ting , Wang Kai-Kuo , Chen Ying-Jie , Xu Zi-Ming , Ma Zi-Yao , Chen Hong-Lu , Wang Qi , Zhao Ran , Sun Xiao-Qing , Li Jiong-Tang
Published: August 2023

Hyperspectral imaging (HSI) has been applied to assess the texture profile analysis (TPA) of processed meat. However, whether the texture profiles of live fish muscle could be assessed using HSI has not been determined. In this study, we evaluated the texture profile of four muscle regions of live common carp by scanning the corresponding skin regions using HSI. We collected skin hyperspectral information from four regions of 387 scaled and live common carp. Eight texture indicators of the muscle corresponding to each skin region were measured. With the skin HSI of live common carp, six machine learning (ML) models were used to predict the muscle texture indicators. Backpropagation artificial neural network (BP-ANN), partial least-square regression (PLSR), and least-square support vector machine (LS-SVM) were identified as the optimal models for predicting the texture parameters of the dorsal (coefficients of determination for prediction (rp) ranged from 0.9191 to 0.9847, and the root-mean-square error for prediction ranged from 0.1070 to 0.3165), pectoral (rp ranged from 0.9033 to 0.9574, and RMSEP ranged from 0.2285 to 0.3930), abdominal (rp ranged from 0.9070 to 0.9776, and RMSEP ranged from 0.1649 to 0.3601), and gluteal (rp ranged from 0.8726 to 0.9768, and RMSEP ranged from 0.1804 to 0.3938) regions. The optimal ML models and skin HSI data were employed to generate visual prediction maps of TPA values in common carp muscles. These results demonstrated that skin HSI and the optimal models can be used to rapidly and accurately determine the texture qualities of different muscle regions in common carp.
Optimal Controls for a Class of Conformable Fractional Evolution Systems
Authors: Liang Yue
Published: August 2023

In the scale of abstract Banach spaces, the existence as well as the controllability are considered for a class of evolution systems involving conformable fractional derivatives. Under essential conditions on nonlinearity, among other conditions, existence theorems are obtained by employing the operator semigroup theory. The existence problem of optimal state-control pairs is further studied by establishing a minimize sequence twice. Two examples are given as applications of the abstract results.
Fitting Early Phases of the COVID-19 Outbreak: A Comparison of the Performances of Used Models
Authors: Sciannameo Veronica , Azzolina Danila , Lanera Corrado , Acar Aslihan Şentürk , Corciulo Maria Assunta , Comoretto Rosanna Irene , Berchialla Paola , Gregori Dario
Published: August 2023

The COVID-19 outbreak involved a spread of prediction efforts, especially in the early pandemic phase. A better understanding of the epidemiological implications of the different models seems crucial for tailoring prevention policies. This study aims to explore the concordance and discrepancies in outbreak prediction produced by models implemented and used in the first wave of the epidemic. To evaluate the performance of the model, an analysis was carried out on Italian pandemic data from February 24, 2020. The epidemic models were fitted to data collected at 20, 30, 40, 50, 60, 70, 80, 90, and 98 days (the entire time series). At each time step, we made predictions until May 31, 2020. The Mean Absolute Error (MAE) and the Mean Absolute Percentage Error (MAPE) were calculated. The GAM model is the most suitable parameterization for predicting the number of new cases; exponential or Poisson models help predict the cumulative number of cases. When the goal is to predict the epidemic peak, GAM, ARIMA, or Bayesian models are preferable. However, the prediction of the pandemic peak could be made carefully during the early stages of the epidemic because the forecast is affected by high uncertainty and may very likely produce the wrong results.
Synthesis, Structure, and Magnetic and Biological Properties of Copper(II) Complexes with 1,3,4-Thiadiazole Derivatives
Authors: Lavrenova Lyudmila G. , Sukhikh Taisiya S. , Glinskaya Lyudmila A. , Trubina Svetlana V. , Zvereva Valentina V. , Lavrov Alexander N. , Klyushova Lyubov S. , Artem’ev Alexander V.
Published: August 2023

New coordination compounds of copper(II) with 2,5-bis(ethylthio)-1,3,4-thiadiazole (L1) and 2,5-bis(pyridylmethylthio)-1,3,4-thiadiazole (L2) with compositions Cu(L1)2Br2, Cu(L1)(C2N3)2, Cu(L2)Cl2, and Cu(L2)Br2 were prepared. The complexes were identified and studied by CHN analysis, infrared (IR) spectroscopy, powder X-Ray diffraction (XRD), and static magnetic susceptibility. The crystal structures of Cu(II) complexes with L1 were determined. The structures of the coordination core of complexes Cu(L2)Cl2 and Cu(L2)Br2 were determined by Extended X-ray absorption fine structure (EXAFS) spectroscopy. Magnetization measurements have revealed various magnetic states in the studied complexes, ranging from an almost ideal paramagnet in Cu(L1)2Br2 to alternating-exchange antiferromagnetic chains in Cu(L1)(C2N3)2, where double dicyanamide bridges provide an unusually strong exchange interaction (J1/kB ≈ −23.5 K; J2/kB ≈ −20.2 K) between Cu(II) ions. The cytotoxic activity of copper(II) complexes with L2 was estimated on the human cell lines of breast adenocarcinoma (MCF-7) and hepatocellular carcinoma (HepG2).
Genome-Wide Exploration and Expression Analysis of the CNGC Gene Family in Eggplant (Solanum melongena L.) under Cold Stress, with Functional Characterization of SmCNGC1a
Authors: Jiang Zheng , Du Lihui , Shen Lei , He Jie , Xia Xin , Zhang Longhao , Yang Xu
Published: August 2023

Eggplant (Solanum melongena L.) is an important economic crop, and to date, there has been no genome-wide identification and analysis of the cyclic nucleotide-gated channel (CNGC) gene family in eggplant. In this study, we identified the CNGC gene family in eggplant, and the results showed that 29 SmCNGC genes were classified into five groups, unevenly distributed across the 12 chromosomes of eggplant. The gene structure and motif analysis indicated that the SmCNGC family proteins may exhibit apparent preferences during evolution. Furthermore, our study revealed the presence of numerous light-responsive elements, hormone-responsive elements, and transcription factor binding sites in the promoter regions of SmCNGC genes, suggesting their significant role in environmental adaptability regulation. Finally, we analyzed the expression patterns of all SmCNGC genes under cold stress and found that SmCNGC1a was significantly upregulated under cold stress. Subcellular localization experiments indicated that this gene is located on the plasma membrane. Subsequently, its importance in the low-temperature response of eggplant was validated through virus-induced gene silencing (VIGS), and its protein interactome was predicted. In summary, our study provides a comprehensive understanding of the function and regulatory mechanisms of the CNGC gene family in eggplant, laying an important foundation for further research on cold adaptation in eggplant.
Comparison of the Bioactive Properties of Human and Bovine Hemoglobin Hydrolysates Obtained by Enzymatic Hydrolysis: Antimicrobial and Antioxidant Potential of the Active Peptide α137-141
Authors: Outman Ahlam , Deracinois Barbara , Flahaut Christophe , Diab Mira Abou , Dhaouefi Jihen , Gressier Bernard , Eto Bruno , Nedjar Naïma
Published: August 2023

This study focuses on the enzymatic hydrolysis of hemoglobin, the main component of cruor that gives blood its red color in mammals. The antibacterial and antioxidant potentials of human hemoglobin hydrolysates were evaluated in comparison to bovine hemoglobin. The results showed strong antimicrobial activity of the peptide hydrolysates against six bacterial strains, independent of the initial substrate concentration level. The hydrolysates also showed strong antioxidant activity, as measured by four different tests. In addition, the antimicrobial and antioxidant activities of the human and bovine hemoglobin hydrolysates showed little or no significant difference, with only the concentration level being the determining factor in their activity. The results of the mass spectrometry study showed the presence of a number of bioactive peptides, the majority of which have characteristics similar to those mentioned in the literature. New bioactive peptides were also identified in human hemoglobin, such as the antibacterial peptides PTTKTYFPHF (α37-46), FPTTKTYFPH (α36-45), TSKYR (α137-141), and STVLTSKYR (α133-141), as well as the antioxidant TSKYR (α137-141). According to these findings, human hemoglobin represents a promising source of bioactive peptides beneficial to the food or pharmaceutical industries.
Ultrasound-Guided Ethanol Ablation for Thyroglossal Duct Cyst: A Review of Technical Issues and Potential as a New Standard Treatment
Authors: Ahn Dongbin
Published: August 2023

The thyroglossal duct cyst (TGDC) is the most common congenital neck mass, accounting for 70–75% of all congenital neck masses. Although the Sistrunk operation has been used as a standard of treatment, it is accompanied by a considerable surgical burden, including the need for general anesthesia, a visible surgical scar on the neck surface, and postoperative complications. Ultrasound-guided ethanol ablation (US-EA) is a minimally invasive and office-based technique that is widely used as a non-surgical treatment for several benign cystic lesions, particularly benign thyroid cysts. Recently, US-EA has also been gaining popularity as a good alternative for TGDC treatment, which is associated with high feasibility, a high safety profile, and favorable treatment outcomes. To our best knowledge, seven studies on the use of EA as a primary treatment option for TGDC have been published since 2011. Although these studies have reported promising results, there is a lack of consensus on several issues regarding the application of EA for TGDC, particularly its detailed techniques and role as a primary treatment. This article aims to provide a comprehensive review of EA for TGDC, addressing technical issues and its possible role as a new standard of treatment for TGDC.