bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations between Personal Attitudes towards COVID-19 and Public Space Soundscape Assessment: An Example from Antwerp, Belgium
Authors: Aletta Francesco , Van Renterghem Timothy
Published: November 2021

The COVID-19 pandemic, and the lockdown events and policies that followed, led to significant changes in the built environment and how it is experienced by people and communities. Among those, variations in the acoustic environments were some of the most noticeable in cities. This study investigated the relationships between the perception of the acoustic environment (i.e., soundscape) and different personal factors such as attitudes towards the pandemic and noise sensitivity, by performing a survey with 109 participants in an urban green public space in Antwerp (Belgium), shortly after most restrictions issued by the government were lifted in September 2020 when the first contamination wave ended. While preliminary in nature, the results of this data collection campaign show that people actively changing their behaviors (using less public transport or cycling more) assessed the soundscapes as less vibrant/exciting. People who were more concerned about the pandemic tended to notice more natural sounds and noise from traffic on nearby local roads. This same subset also put a bigger importance on the environmental quality of the public space than in the pre-pandemic period. Noise sensitivity also played a role, as an association was found between more-than-average noise sensitive persons and those more worried regarding the pandemic. Overall, the findings of this study confirm that at least part of the people have started to perceive the public space, including its soundscape, differently since the start of the pandemic.
‘It’s Easily the Lowest I’ve Ever, Ever Got to’: A Qualitative Study of Young Adults’ Social Isolation during the COVID-19 Lockdowns in the UK
Authors: Dedryver Chloe C. , Knai Cécile
Published: November 2021

(1) Background: Social connectivity is key to young people’s mental health. Local assets facilitate social connection, but were largely inaccessible during the pandemic. This study consequently investigates the social isolation of young adults and their use of local assets during the COVID-19 lockdowns in the UK. (2) Methods: Fifteen semi-structured Zoom interviews were undertaken with adults aged 18–24 in the UK. Recruitment took place remotely, and transcripts were coded and analysed thematically. (3) Results: Digital assets were key to young people’s social connectivity, but their use was associated with stress, increased screen time and negative mental health outcomes. The lockdowns impacted social capital, with young people’s key peripheral networks being lost, yet close friendships being strengthened. Finally, young people’s mental health was greatly affected by the isolation, but few sought help, mostly out of a desire to not overburden the NHS. (4) Conclusions: This study highlights the extent of the impact of the pandemic isolation on young people’s social capital and mental health. Post-pandemic strategies targeting mental health system strengthening, social isolation and help-seeking behaviours are recommended.
Frailty Related to the Exposure to Particulate Matter and Ozone: The Korean Frailty and Aging Cohort Study
Authors: Shin Jinyoung , Choi Jaekyung
Published: November 2021

This study aims to identify the association between the concentration of particulate matter <2.5 μm (PM2.5), <10 μm (PM10), and ozone (O3) and frailty. The Korean Frailty Scale (KFS, 0–6 points) assessing physical, psychological, and social frailty, was applied to 2912 community-dwelling older adults between April 2016 and December 2017. Daily average concentrations of PM2.5, PM10, and O3 (2015–2017) were obtained and matched with the residential areas. The frailty risk associated with exposure to PM2.5, PM10, and O3 was evaluated using multiple logistic regression after adjusting for age, sex, BMI, lifestyle, socioeconomic status, and comorbidity. Participants were categorized into robust (0 points, 28.7%), pre-frail (1–2 points, 50.1%), and frail (≥3 points, 21.2%) groups. Each 1 μg/m3 increase of PM2.5 and PM10 increased the odds ratios (ORs) and 95% confidence intervals (CIs) of the frail group compared to the robust group: 1.055 (1.002, 1.112) and 1.095 (1.060, 1.131), and the pre-frail group: 1.053 (1.017, 1.090) and 1.062 (1.037, 1.087), respectively. Each 1-ppb increase of O3 increased the OR (95% CI) of the frail group: 1.041 (1.023, 1.059) and the pre-frail group: 1.005 (0.985, 1.025). PM2.5, PM10, and O3 may be associated dose-dependently with the frailty.
Mild Muscle Mitochondrial Fusion Distress Extends Drosophila Lifespan through an Early and Systemic Metabolome Reorganization
Authors: Tapia Andrea , Palomino-Schätzlein Martina , Roca Marta , Lahoz Agustín , Pineda-Lucena Antonio , López del Amo Víctor , Galindo Máximo Ibo
Published: November 2021

In a global aging population, it is important to understand the factors affecting systemic aging and lifespan. Mitohormesis, an adaptive response caused by different insults affecting the mitochondrial network, triggers a response from the nuclear genome inducing several pathways that promote longevity and metabolic health. Understanding the role of mitochondrial function during the aging process could help biomarker identification and the development of novel strategies for healthy aging. Herein, we interfered the muscle expression of the Drosophila genes Marf and Opa1, two genes that encode for proteins promoting mitochondrial fusion, orthologues of human MFN2 and OPA1. Silencing of Marf and Opa1 in muscle increases lifespan, improves locomotor capacities in the long term, and maintains muscular integrity. A metabolomic analysis revealed that muscle down-regulation of Marf and Opa1 promotes a non-autonomous systemic metabolome reorganization, mainly affecting metabolites involved in the energetic homeostasis: carbohydrates, lipids and aminoacids. Interestingly, the differences are consistently more evident in younger flies, implying that there may exist an anticipative adaptation mediating the protective changes at the older age. We demonstrate that mild mitochondrial muscle disturbance plays an important role in Drosophila fitness and reveals metabolic connections between tissues. This study opens new avenues to explore the link of mitochondrial dynamics and inter-organ communication, as well as their relationship with muscle-related pathologies, or in which muscle aging is a risk factor for their appearance. Our results suggest that early intervention in muscle may prevent sarcopenia and promote healthy aging.
The Different Molecular Code in Generation of Dopaminergic Neurons from Astrocytes and Mesenchymal Stem Cells
Authors: Wang Nana , Ji Xingrui , Wu Yue , Zhou Shaocong , Peng Huiyu , Wang Jingwen , Yu Shuang , Zhang Jingzhong
Published: November 2021

Transplantation of exogenous dopaminergic (DA) neurons is an alternative strategy to replenish DA neurons that have lost along the course of Parkinson’s disease (PD). From the perspective of ethical acceptation, the source limitations, and the intrinsic features of PD pathology, astrocytes (AS) and mesenchymal stem cells (MSCs) are the two promising candidates of DA induction. In the present study, we induced AS or MSCs primary culture by the combination of the classical transcription-factor cocktails Mash1, Lmx1a, and Nurr1 (MLN), the chemical cocktails (S/C/D), and the morphogens SHH, FGF8, and FGF2 (S/F8/F2); the efficiency of induction into DA neurons was further analyzed by using immunostaining against the DA neuronal markers. AS could be efficiently converted into the DA neurons in vitro by the transcriptional regulation of MLN, and the combination with S/C/D or S/F8/F2 further increased the conversion efficiency. In contrast, MSCs from umbilical cord (UC-MSCs) or adipose tissue (AD-MSCs) showed moderate TH immunoreactivity after the induction with S/F8/F2 instead of with MLN or S/C/D. Our data demonstrated that AS and MSCs held lineage-specific molecular codes on the induction into DA neurons and highlighted the unique superiority of AS in the potential of cell replacement therapy for PD.
Possible Role of Pineal and Extra-Pineal Melatonin in Surveillance, Immunity, and First-Line Defense
Authors: Markus Regina P. , Sousa Kassiano S. , da Silveira Cruz-Machado Sanseray , Fernandes Pedro A. , Ferreira Zulma S.
Published: November 2021

Melatonin is a highly conserved molecule found in prokaryotes and eukaryotes that acts as the darkness hormone, translating environmental lighting to the whole body, and as a moderator of innate and acquired defense, migration, and cell proliferation processes. This review evaluates the importance of pineal activity in monitoring PAMPs and DAMPs and in mounting an inflammatory response or innate immune response. Activation of the immune–pineal axis, which coordinates the pro-and anti-inflammatory phases of an innate immune response, is described. PAMPs and DAMPs promote the immediate suppression of melatonin production by the pineal gland, which allows leukocyte migration. Monocyte-derived macrophages, important phagocytes of microbes, and cellular debris produce melatonin locally and thereby initiate the anti-inflammatory phase of the acute inflammatory response. The role of locally produced melatonin in organs that directly contact the external environment, such as the skin and the gastrointestinal and respiratory tracts, is also discussed. In this context, as resident macrophages are self-renewing cells, we explore evidence indicating that, besides avoiding overreaction of the immune system, extra-pineal melatonin has a fundamental role in the homeostasis of organs and tissues.
New Insights on the Role of Connexins and Gap Junctions Channels in Adipose Tissue and Obesity
Authors: González-Casanova Jorge Enrique , Durán-Agüero Samuel , Caro-Fuentes Nelson Javier , Gamboa-Arancibia Maria Elena , Bruna Tamara , Bermúdez Valmore , Rojas-Gómez Diana Marcela
Published: November 2021

Due to the inability to curb the excessive increase in the prevalence of obesity and overweight, it is necessary to comprehend in more detail the factors involved in the pathophysiology and to appreciate more clearly the biochemical and molecular mechanisms of obesity. Thus, understanding the biological regulation of adipose tissue is of fundamental relevance. Connexin, a protein that forms intercellular membrane channels of gap junctions and unopposed hemichannels, plays a key role in adipogenesis and in the maintenance of adipose tissue homeostasis. The expression and function of Connexin 43 (Cx43) during the different stages of the adipogenesis are differentially regulated. Moreover, it has been shown that cell–cell communication decreases dramatically upon differentiation into adipocytes. Furthermore, inhibition of Cx43 degradation or constitutive overexpression of Cx43 blocks adipocyte differentiation. In the first events of adipogenesis, the connexin is highly phosphorylated, which is likely associated with enhanced Gap Junction (GJ) communication. In an intermediate state of adipocyte differentiation, Cx43 phosphorylation decreases, as it is displaced from the membrane and degraded through the proteasome; thus, Cx43 total protein is reduced. Cx is involved in cardiac disease as well as in obesity-related cardiovascular diseases. Different studies suggest that obesity together with a high-fat diet are related to the production of remodeling factors associated with expression and distribution of Cx43 in the atrium.
Tau Cleavage Contributes to Cognitive Dysfunction in Strepto-Zotocin-Induced Sporadic Alzheimer’s Disease (sAD) Mouse Model
Authors: Latina Valentina , Giacovazzo Giacomo , Calissano Pietro , Atlante Anna , La Regina Federico , Malerba Francesca , Dell’Aquila Marco , Stigliano Egidio , Balzamino Bijorn Omar , Micera Alessandra , Coccurello Roberto , Amadoro Giuseppina
Published: November 2021

Tau cleavage plays a crucial role in the onset and progression of Alzheimer’s Disease (AD), a widespread neurodegenerative disease whose incidence is expected to increase in the next years. While genetic and familial forms of AD (fAD) occurring early in life represent less than 1%, the sporadic and late-onset ones (sAD) are the most common, with ageing being an important risk factor. Intracerebroventricular (ICV) infusion of streptozotocin (STZ)—a compound used in the systemic induction of diabetes due to its ability to damage the pancreatic β cells and to induce insulin resistance—mimics in rodents several behavioral, molecular and histopathological hallmarks of sAD, including memory/learning disturbance, amyloid-β (Aβ) accumulation, tau hyperphosphorylation, oxidative stress and brain glucose hypometabolism. We have demonstrated that pathological truncation of tau at its N-terminal domain occurs into hippocampi from two well-established transgenic lines of fAD animal models, such as Tg2576 and 3xTg mice, and that it’s in vivo neutralization via intravenous (i.v.) administration of the cleavage-specific anti-tau 12A12 monoclonal antibody (mAb) is strongly neuroprotective. Here, we report the therapeutic efficacy of 12A12mAb in STZ-infused mice after 14 days (short-term immunization, STIR) and 21 days (long-term immunization regimen, LTIR) of i.v. delivery. A virtually complete recovery was detected after three weeks of 12A12mAb immunization in both novel object recognition test (NORT) and object place recognition task (OPRT). Consistently, three weeks of this immunization regimen relieved in hippocampi from ICV-STZ mice the AD-like up-regulation of amyloid precursor protein (APP), the tau hyperphosphorylation and neuroinflammation, likely due to modulation of the PI3K/AKT/GSK3-β axis and the AMP-activated protein kinase (AMPK) activities. Cerebral oxidative stress, mitochondrial impairment, synaptic and histological alterations occurring in STZ-infused mice were also strongly attenuated by 12A12mAb delivery. These results further strengthen the causal role of N-terminal tau cleavage in AD pathogenesis and indicate that its specific neutralization by non-invasive administration of 12A12mAb can be a therapeutic option for both fAD and sAD patients, as well as for those showing type 2 diabetes as a comorbidity.
Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition
Authors: Pedretti Marco , Bombardi Luca , Conter Carolina , Favretto Filippo , Dominici Paola , Astegno Alessandra
Published: November 2021

Centrins are a family of small, EF hand-containing proteins that are found in all eukaryotes and are often complexed with centrosome-related structures. Since their discovery, centrins have attracted increasing interest due to their multiple, diverse cellular functions. Centrins are similar to calmodulin (CaM) in size, structure and domain organization, although in contrast to CaM, the majority of centrins possess at least one calcium (Ca2+) binding site that is non-functional, thus displaying large variance in Ca2+ sensing abilities that could support their functional versatility. In this review, we summarize current knowledge on centrins from both biophysical and structural perspectives with an emphasis on centrin-target interactions. In-depth analysis of the Ca2+ sensing properties of centrins and structures of centrins complexed with target proteins can provide useful insight into the mechanisms of the different functions of centrins and how these proteins contribute to the complexity of the Ca2+ signaling cascade. Moreover, it can help to better understand the functional redundancy of centrin isoforms and centrin-binding proteins.
Phenology, Seasonal Abundance, and Host-Plant Association of Spittlebugs (Hemiptera: Aphrophoridae) in Vineyards of Northwestern Italy
Authors: Bodino Nicola , Demichelis Stefano , Simonetto Anna , Volani Stefania , Saladini Matteo Alessandro , Gilioli Gianni , Bosco Domenico
Published: November 2021

Spittlebugs (Hemiptera: Aphrophoridae) are the vectors of the bacterium Xylella fastidiosa (Xf) in Europe. Xf may cause severe epidemics in cultivated plants, including grapevines. To assess the threat represented by the bacterium to grapevines, detailed information on the vectors’ phenology, density, and ecology in vineyards is needed. The aim of the present work was to describe spittlebug diversity, phenology, and host-plant association in the vineyard agroecosystem. Two separate field surveys of nymphal and adult spittlebug populations, i.e., a two-year survey of a single site and a one-year survey of three sites, were performed in vineyards of northwestern Italy in three consecutive years. Philaenus spumarius was the most common species, reaching average nymph densities on herbaceous cover up to 60–130 nymphs/m2. Adults were sampled on grapevines from May to September, with a peak in June (up to 0.43 insects/sweep). Herbaceous cover was colonized after egg hatching and in late summer for oviposition, while wild woody hosts represented a refuge during summer. The results show that spittlebugs can reach high population levels in vineyards, at least in the areas where the ground is covered by herbaceous plants for the whole season and the use of insecticides is moderate. The extended presence of P. spumarius adults on grapevines represents a serious risk factor for the spread of Xf. The scenarios of Xf establishment in vineyards in northwestern Italy and Europe are discussed in relation to the abundance, phenology, and plant association of spittlebugs.
The Efficacy, Safety and Satisfaction Associated with Switching from Brinzolamide 1% and Brimonidine 0.1% to a Fixed Combination of Brinzolamide 1% and Brimonidine 0.1% in Glaucoma Patients
Authors: Onoe Hiromitsu , Hirooka Kazuyuki , Nagayama Mikio , Hirota Atsushi , Mochizuki Hideki , Sagara Takeshi , Suzuki Katsuyoshi , Okumichi Hideaki , Kiuchi Yoshiaki
Published: November 2021

We evaluated glaucoma patients for the efficacy, safety and satisfaction associated with switching from brinzolamide 1% and brimonidine 0.1% to a fixed combination of brinzolamide 1% and brimonidine 0.1%. A total of 22 glaucoma patients were enrolled and completed this prospective, nonrandomized study that evaluated patients who underwent treatment with at least brinzolamide 1% and brimonidine 0.1%. Patients on brinzolamide 1% and brimonidine 0.1% were switched to a brinzolamide/brimonidine fixed-combination ophthalmic suspension (BBFC). Evaluations of intraocular pressure (IOP), superficial punctate keratopathy (SPK) and conjunctival hyperemia were conducted at baseline and at 4 and 12 weeks. The Treatment Satisfaction Questionnaire for Medication-9 (TSQM-9) was utilized to assess the change in treatment satisfaction. At baseline and at 4 and 12 weeks, the IOP was 15.0 ± 4.1, 14.8 ± 4.1 and 14.8 ± 4.1 mmHg, respectively. There were no significant differences observed at any of the time points. However, the SPK score significantly decreased at 12 weeks, even though no significant differences were observed for the conjunctival hyperemia incidence at any of the time points. After switching from brinzolamide 1% and brimonidine 0.1% to BBFC, there was a significant increase in the TSQM-9 score for convenience and global satisfaction. Both an improvement in the degree of SPK and an increase in treatment satisfaction occurred after switching from brinzolamide 1% and brimonidine 0.1% to BBFC, even though there were sustained IOP values throughout the 12-week evaluation period.
Clinical Features and Natural History in a Cohort of Chinese Patients with RPE65-Associated Inherited Retinal Dystrophy
Authors: Shi Jie , Xu Ke , Hu Jian-Ping , Xie Yue , Zhang Xin , Zhang Xiao-Hui , Jin Zi-Bing , Li Yang
Published: November 2021

RPE65-associated inherited retinal dystrophy (RPE65-IRD) is an early-onset retinal degeneration. The aim of this study was to describe the clinical features and natural course of this disease in a Chinese patient cohort with RPE65 biallelic variants. Thirty patients from 29 unrelated families with biallelic disease-causing RPE65 variants underwent full ophthalmic examinations. Thirteen were followed up over time. An additional 57 Chinese cases from 49 families were retrieved from the literature to analyze the relationship between best-corrected visual acuity (BCVA) and age. Our 30 patients presented age-dependent phenotypic characteristics. Multiple white dots were a clinical feature of young patients, while maculopathy, epiretinal membrane, and bone spicules were common in adult patients. Among the 84 patients, BCVA declined with age in a nonlinear, positive-acceleration relationship (p < 0.001). All patients older than 40 years met the WHO standard for low vision. Longitudinal observation revealed a slower visual acuity loss in patients younger than 20 years than those in their third or fourth decade of life. Our study detailed the clinical features and natural course of disease in Chinese patients with RPE65-IRD. Our results indicated that these patients have a relatively stable BCVA in childhood and adolescence, but eyesight deteriorates rapidly in the third decade of life. These findings may facilitate the implementation of gene therapy in China.
Association between Lipid Levels and Risk for Different Types of Aneurysms: A Mendelian Randomization Study
Authors: Chen Yanghui , Huang Man , Xuan Yunling , Li Ke , Xu Xin , Wang Linlin , Sun Yang , Xiao Lei , Xu Ping , Kong Wei , Wang Dao Wen
Published: November 2021

Background: Although the associations between serum lipid levels and aneurysms have been investigated in epidemiological studies, causality remains unknown. Thus, this study aimed to investigate the causal relationships of serum high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), and triglyceride (TG) levels on five types of aneurysms, using genetic variants associated with four lipid traits as instrumental variables in a Mendelian randomization (MR) analysis. Methods: We performed two-sample Mendelian randomization (MR) analyses to evaluate the associations of HDL-C, LDL-C, TC, and TG levels with risks for five types of aneurysms and those of LDL-C- (HMGCR, NPC1L1, PCSK9, CETP, and LDLR) and TG-lowering targets (ANGPTL3 and LPL) with aneurysms. Results: The sample sizes of the included studies ranged from nearly 80,000 to 410,000. We found inverse associations between genetically predicted HDL-C levels and aortic (OR = 0.74, 95% CI = 0.65–0.85) and abdominal aortic aneurysms (0.58, 0.45–0.75). A 1-SD increase in LDL-C and TC levels was associated with increased risks for aortic (1.41, 1.26–1.58 and 1.36, 1.18–1.56, respectively) and abdominal aortic aneurysms (1.82, 1.48–2.22 and 1.55, 1.25–1.93, respectively). TG levels were significantly associated with aortic (1.36, 1.18–1.56) and lower extremity artery aneurysms (2.76, 1.48–5.14), but limited to cerebral aneurysm (1.23, 1.06–1.42). Secondary analyses revealed a relationship between genetically proxied LDL-C-lowering targets and all types of aneurysms; however, the drug targets remained heterogeneous. We found a weak association between TG-lowering therapies and aortic (ANGPTL3, 0.51, 0.29–0.89) and abdominal aortic aneurysms (LPL, 0.64, 0.44–0.94). Conclusion: According to genetic evidence, lipid dysfunction is a causal risk factor for aneurysms. Lipid-lowering drugs may be a potential effective strategy in preventing and managing aneurysms.
Cross-Cultural Analysis of the Consumer Profile of Hunting Tourism and Trophy Hunting from Tradition to Protected Areas: Study Case on Romania and Spain
Authors: Oltean Flavia Dana , Gabor Manuela Rozalia
Published: November 2021

Hunting has major importance from many perspectives: As a product of leisure and recreation, as a tool for conservation and wildlife management, as the main economic activity in rural areas, or as a cultural heritage and traditional activity for countries around the world, especially for countries in Europe and Africa. Therefore, this research fills a gap in the literature and offers a cross-cultural opinion and perceptions of 198 hunters from Romania and Spain. The aim of the paper is to analyze the perceptions and opinions of hunters regarding hunting tourism through an online self-administrated questionnaire by convenience sampling using hunters associations from these countries. Among the values that identify hunting as an activity, hunters highlight the human values (friendship, company, ethics), ecological values (love of nature associated with hunting as a tool to understand and enjoy the natural environment), and social values (resources generated, hobby, effort). The respondents can self-criticize some components and aspects of hunting groups. Hunters believe that the future of this sector is moving towards commercial hunting, associated with purchasing power to ensure results. Regardless of the nationality of the hunters, their values related to this sector are similar.
Unraveling Risk Networks of Cultivated Land Protection: An Exploratory Stakeholder-Oriented Case Study in Xiliuhe Town, Hubei Province, China
Authors: Guo Zhaoxia , Guo Qinqin , Cai Yujie , Wang Ge
Published: November 2021

The protection of cultivated land plays an important role in ensuring food security, maintaining social stability, and promoting economic development. The protection of cultivated land involves a range of stakeholders (e.g., governments at different levels, farmers, and land-use organizations) and entails intertwined risk factors (e.g., to economic, environmental, social, and political factors). Therefore, it is crucial to identify and assess key stakeholders and associated risks to better align land protection policies. However, previous studies of risk are fragmented, and there has been little research targeting the complex interactions among risk factors in the protection of cultivated land. Taking Xiliuhe Town as an example, this study analyzes complex and intertwined risk factors from a network perspective in an in-depth case study in a major grain-producing area. The results show that: (1) the risk-factor network of cultivated land protection is relatively sparse, with a total of 142 nodes and 253 links; (2) local governments and land flow-out farmers are the core stakeholders, with strong power and connectivity in the network; (3) taking into account key stakeholders and associated risk factors, a framework for mitigating risks is developed, and a network simulation is performed. Using the simulation results, the effectiveness of the risk-mitigation strategies is assessed and validated. These results shed new light on cultivated land protection and sustainable agricultural development in emerging countries.
A Plausible Proposition of CCL20-Related Mechanism in Fusobacterium nucleatum-Associated Oral Carcinogenesis
Authors: Mandal Devi Prasad , Mohanty Neeta , Behera Paresh Kumar , Gopinath Divya , Panda Sasmita , Al-Kheraif Abdulaziz A. , Divakar Darshan Devang , Anil Sukumaran , Panda Swagatika
Published: November 2021

Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of Fusobacterium nucleatum in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in Fusobacterium nucleatum-positive OSCC is another objective. Methodology: Tissues from 50 OSCC patients and 30 healthy oral tissues were collected. The prevalence of Fusobacterium nucleatum was evaluated in both tumour and healthy tissue by polymerase chain reaction. The immunohistochemistry of OSCC tissues was conducted to evaluate the difference in the expression of CCL20 between Fusobacterium nucleatum-positive and -negative OSCC tissues. Results: Fusobacterium nucleatum was significantly (p < 0.001) prevalent in OSCC tissues (74%), compared to healthy tissues (26%). No association of Fusobacterium nucleatum or CCL20 immuno-expression with any clinical or histopathological features of OSCC was observed. While the intensity of CCL20 immuno-expression did not differ (p = 0.053), the CCL20-positive cell population was significantly different (p = 0.034) between Fusobacterium nucleatum-positive and -negative OSCC. Conclusion: Fusobacterium nucleatum is possibly prevalent in oral cancer tissues in the Indian population. By using immunohistochemistry, this is the first study to propose that the carcinogenesis in Fusobacterium nucleatum-positive OSCC may be CCL20-related. The findings enrich the knowledge of mechanisms involved in Fusobacterium nucleatum-mediated oral carcinogenesis.
Flux Estimator for Salient Pole Synchronous Machines Driven by the Cycloconverter Based on Enhanced Current and Voltage Model of the Machine with Fuzzy Logic Transition
Authors: Cikač Dominik , Turk Nikola , Bulić Neven , Barbanti Stefano
Published: November 2021

Flux estimation is a key feature of the field-oriented control for the electrically excited synchronous machine which enables the high-performance, high-dynamic drive behavior. In this work, an electrically excited synchronous machine flux estimator based on a current and voltage model is proposed. In this case, the transition between the estimators is done with a fuzzy logic set of rules. The flux estimator based on the current model of the machine in this paper considers the saturation and cross-coupling effect in both axis and it is suitable for applications where a limited amount of the machine data is available. The flux estimator based on the voltage model is specially designed for the drives where high voltage and current ripple is present under normal operating conditions, e.g., like in cycloconverter applications. To exploit all the advantages of both models, a fuzzy logic transition is proposed based on multiple choices which manages the transition between the models based on a speed and torque reference. The proposed flux estimator is experimentally verified on a cycloconverter fed salient-pole electrically excited synchronous machine. The experimental results clearly show that the proposed flux estimator enables the accurate and stable operating conditions for different operating points of the cycloconverter-fed salient-pole electrically excited synchronous machine.
Comparison of Molecular Geometry Optimization Methods Based on Molecular Descriptors
Authors: Bálint Donatella , Jäntschi Lorentz
Published: November 2021

Various methods (Hartree–Fock methods, semi-empirical methods, Density Functional Theory, Molecular Mechanics) used to optimize a molecule structure feature the same basic approach but differ in the mathematical approximations used. The geometry optimization procedure calculates the energy at an initial geometry of a molecule and then proceeds to search a new geometry with a lower energy. Using the 3D structures collected from the PubChem database, 20 amino acid geometry optimization calculations were performed with several methods. The purpose of the study was to analyze these methods (39) to find the relationship between them and to determine which to use under different circumstances. Cluster analysis and principal component analysis were performed to evaluate the similarities between the different methods. The results after the analysis can classified into three main groups and can be selected accordingly to solve different types of problems.
Efficient Recovery of Noble Metal Ions (Pd2+, Ag+, Pt2+, and Au3+) from Aqueous Solutions Using N,N'-Bis(salicylidene)ethylenediamine (Salen) as an Extractant (Classic Solvent Extraction) and Carrier (Polymer Membranes)
Authors: Witt Katarzyna , Kaczorowska Małgorzata A. , Bożejewicz Daria , Urbaniak Włodzimierz
Published: November 2021

This paper presents the results of the first application of N,N'-bis(salicylidene)ethylenediamine (salen) as an extractant in classical liquid–liquid extraction and as a carrier in membrane processes designed for the recovery of noble metal ions (Pd2+, Ag+, Pt2+, and Au3+) from aqueous solutions. In the case of the utilization of membranes, both sorption and desorption were investigated. Salen has not been used so far in the sorption processes of precious metal ions. Recovery experiments were performed on single-component solutions (containing only one type of metal ions) and polymetallic solutions (containing ions of all four metals). The stability constants of the obtained complexes were determined spectrophotometrically. In contrast, electrospray ionization high-resolution mass spectrometry (ESI-HRMS) was applied to examine the elemental composition and charge of the generated complexes of chosen noble metal ions and salen molecules. The results show the great potential of N,N'-bis(salicylidene)ethylenediamine as both an extractant and a carrier. In the case of single-component solutions, the extraction percentage was over 99% for all noble metal ions (molar ratio M:L of 1:1), and in the case of a polymetallic solution, it was the lowest, but over 94% for platinum ions and the highest value (over 99%) for gold ions. The percentages of sorption (%Rs) of metal ions from single-component solutions using polymer membranes containing N,N'-bis(salicylidene)ethylenediamine as a carrier were highest after 24 h of the process (93.23% for silver(I) ions, 74.99% for gold(III) ions, 69.11% and 66.13% for palladium(II) and platinum(II) ions, respectively), similar to the values obtained for the membrane process conducted in multi-metal solutions (92.96%, 84.26%, 80.94%, and 48.36% for Pd(II), Au(III), Ag(I), and Pt(II) ions, respectively). The percentage of desorption (%Rdes) was very high for single-component solutions (the highest, i.e., 99%, for palladium solution and the lowest, i.e., 88%, for silver solution), while for polymetallic solutions, these values were slightly lower (for Pt(II), it was the lowest at 63.25%).
Improvement of Erosion-Corrosion Behavior of AISI 420 Stainless Steel by Ion-Assisted Deposition ZrN Coatings
Authors: Xi Yuntao , Wan Lin , Hou Jungang , Wang Zhiyong , Wang Lei , Yang Daoyong , Liu Daoxin , Lei Shubin
Published: November 2021

In this paper, a pragmatic technique has been developed to evaluate the erosion-corrosion behavior of three kinds of ZrN coatings (i.e., monolayer, multilayer, and gradient layers) which were deposited on AISI 420 martensitic stainless steel using an ion-assisted deposition technology. Among them, the monolayer coating refers to the coating with no change in composition and structure, the multilayer coating refers to the coating with alternating change of Zr/ZrN, and the gradient coating refers to the ZrN coating by increasing N2 partial pressure gradually. The morphology, composition, and microhardness of these ZrN coatings were examined by means of integrating the scanning electron microscopy (SEM), X-ray diffraction (XRD), and Knoop hardness measurements, while anodic polarization tests and salt fog spray tests in a simulated industrial environment have been performed to evaluate and identify the corrosion mechanisms of these coatings. The surface microhardness and corrosion resistance of the AISI420 martensitic stainless steel is found to be significantly improved by depositing the ion-assisted deposition ZrN coatings. The study indicates that the erosion-corrosion behavior in the slurry is the result of the synergistic effect of small-angle erosion and acid solution corrosion. Three ZrN coatings hinder the slurry erosion-corrosion behavior from two aspects (i.e., erosion resistance of small-angle particles as well as corrosion resistance of the substrate), thereby significantly improving the erosion-corrosion resistance of AISI 420 stainless steel. In addition, the ZrN gradient coatings show a much better erosion-corrosion resistance than that of the monolayer/multilayer ZrN coating because they have excellent crack resistance, bearing capacity, and electrochemical performance.
Gender-Specific Metabolomics Approach to Kidney Cancer
Authors: Deja Stanisław , Litarski Adam , Mielko Karolina Anna , Pudełko-Malik Natalia , Wojtowicz Wojciech , Zabek Adam , Szydełko Tomasz , Młynarz Piotr
Published: November 2021

Renal cell carcinoma (RCC) is the most common form of kidney malignancy. RCC is more common among men with a 2/1 male/female incidence ratio worldwide. Given the underlying epidemiological differences in the RCC incidence between males and females, we explored the gender specific 1H NMR serum metabolic profiles of RCC patients and their matched controls. A number of differential metabolites were shared by male and female RCC patients. These RCC specific changes included lower lactate, threonine, histidine, and choline levels together with increased levels of pyruvate, N-acetylated glycoproteins, beta-hydroxybutyrate, acetoacetate, and lysine. Additionally, serum lactate/pyruvate ratio was a strong predictor of RCC status regardless of gender. Although only moderate changes in metabolic profiles were observed between control males and females there were substantial gender related differences among RCC patients. Gender specific metabolic features associated with RCC status were identified suggesting that different metabolic panels could be leveraged for a more precise diagnostic.
Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries
Authors: Gómez Saúl , Quezada Valentina , Quiroz Isabella , Muñoz-Camargo Carolina , Osma Johann , Reyes Luis , Cruz Juan
Published: November 2021

The discovery of new membrane-active peptides (MAPs) is an area of considerable interest in modern biotechnology considering their ample applicability in several fields ranging from the development of novel delivery vehicles (via cell-penetrating peptides) to responding to the latent threat of antibiotic resistance (via antimicrobial peptides). Different strategies have been devised for such discovery process, however, most of them involve costly, tedious, and low-efficiency methods. We have recently proposed an alternative route based on constructing a non-rationally designed library recombinantly expressed on the yeasts’ surfaces. However, a major challenge is to conduct a robust and high-throughput screening of possible candidates with membrane activity. Here, we addressed this issue by putting forward low-cost microfluidic platforms for both the synthesis of Giant Unilamellar Vesicles (GUVs) as mimicking entities of cell membranes and for providing intimate contact between GUVs and homologues of yeasts expressing MAPs. The homologues were chitosan microparticles functionalized with the membrane translocating peptide Buforin II, while intimate contact was through passive micromixers with different channel geometries. Both microfluidic platforms were evaluated both in silico (via Multiphysics simulations) and in vitro with a high agreement between the two approaches. Large and stable GUVs (5–100 µm) were synthesized effectively, and the mixing processes were comprehensively studied leading to finding the best operating parameters. A serpentine micromixer equipped with circular features showed the highest average encapsulation efficiencies, which was explained by the unique mixing patterns achieved within the device. The microfluidic devices developed here demonstrate high potential as platforms for the discovery of novel MAPs as well as for other applications in the biomedical field such as the encapsulation and controlled delivery of bioactive compounds.
Topological Design of Multi-Material Compliant Mechanisms with Global Stress Constraints
Authors: Zhan Jinqing , Li Yifeng , Luo Zhen , Liu Min
Published: November 2021

This paper presents an approach for the topological design of multi-material compliant mechanisms with global stress constraints. The element stacking method and the separable stress interpolation scheme are applied to calculate the element stiffness and element stress of multi-material structures. The output displacement of multi-material compliant mechanisms is maximized under the constraints of the maximum stress and the structural volume of each material. The modified P-norm method is applied to aggregate the local von Mises stress constraints for all the finite elements to a global stress constraint. The sensitivities are calculated by the adjoint method, and the method of moving asymptotes is utilized to update the optimization problem. Several numerical examples are presented to demonstrate the effectiveness of the proposed method. The appearance of the de facto hinges in the optimal mechanisms can be suppressed effectively by using the topology optimization model with global stress constraints, and the stress constraints for each material can be met.
Mineralogical Characterization of Manganese Oxide Minerals of the Devonian Xialei Manganese Deposit
Authors: Niu Sida , Zhao Liqun , Lin Xiaoju , Chen Tong , Wang Yingchao , Mo Lingchao , Niu Xianglong , Wu Huaying , Zhang Min , Huizenga Jan Marten , Long Peng
Published: November 2021

The Guangxi Zhuang Autonomous Region is an important manganese ore district in Southwest China, with manganese ore resource reserves accounting for 23% of the total manganese ore resource reserves in China. The Xialei manganese deposit (Daxin County, Guangxi) is the first super-large manganese deposit discovered in China. The Mn oxide in the supergene oxidation zone of the Xialei deposit was characterized using scanning electron microscopy (SEM), energy spectrometer (EDS), transmission electron microscopy (TEM, HRTEM), and X-ray diffraction analysis (XRD). The Mn oxides have a gray-black/steel-gray color, a semi-metallic-earthy luster, and appear as oolitic, pisolitic, banded, massive, and cellular textures. Scanning electron microscopy images show that the manganese oxide minerals are present as fine-spherical particles with an earthy surface. TEM and HRTEM indicate the presence of oriented bundled and staggered nanorods, and nanopores between the crystals. The Mn oxide ore can be classified into two textural types: (1) oolitic and pisolitic (often with annuli) Mn oxide, and (2) massive Mn oxide. Pyrolusite, cryptomelane, and hollandite are the main Mn oxide minerals. The potassium contents of cryptomelane and pyrolusite are discussed. The unit cell parameters of pyrolusite are refined.
Early Devonian Arc-Related Volcanic Rocks in the Haerdaban, North Margin of the Yili Block: Constraint on the Southward Subduction of the Junggar Ocean
Authors: Chen Youxin , Zhu Shengqiang , Pei Xianzhi , He Lei , Zhao Jun , Bulong Bate , Wang Meng , Zhao Shaowei , Zhou Hai
Published: November 2021

The origin and tectonic implication of Early–Middle Devonian magmatism in the northern margin of YB (Yili Block) remain enigmatic and are important for understanding Late Paleozoic evolution of the Junggar Ocean and southern Kazakhstan Orocline. Here, we present the systematic study of whole-rock geochemical and Sr–Nd isotope features as well as U–Pb–Hf isotope characteristics of zircon crystals for newly identified Early Devonian volcanic rocks from the northern margin of YB. The volcanic rocks are composed of rhyolite, rhyolite porphyry, and rhyolitic tuff. Zircon U-Pb age dating indicates they were formed at ca. 407~418 Ma. They have high SiO2 (70.16–77.52 wt.%) and alkali (5.10–9.56 wt.%) contents, and high Zr + Nb + Ce + Y content (~456 ppm), indicative of A-type magma. Their relative depletion of Nb, Ta, and Ti, and enrichment of LILEs show arc affinity. Their low initial 87Sr/86Sr ratios (0.699708–0.709822) and negative εNd(t) values (−1.8 to −4.0) indicate a mainly continental magma source and their positive εHf(t)values (+6.13 to +14.81) are possibly due to the garnet effect. All these above reveal that volcanic rocks were generated by re-melting of lower crust under a high temperature condition, which was induced by long-lived heat accumulation with no or minimal basalt flux. Combined with active continental margin inference evidenced by contemporaneous sedimentary rocks, we attribute the generation of the volcanic rocks to a continental arc setting related to the southward subduction of Junggar oceanic crust. Thus, we infer the Early–Middle Devonian arc-related magmatic rocks in the northern margin of YB are eastward counterparts of the southern limb of the Devonian Volcanic Belt, which resulted from a relatively steady-state southward subduction.
Anticonvulsive Effects of Chondroitin Sulfate on Pilocarpine and Pentylenetetrazole Induced Epileptogenesis in Mice
Authors: Singh Shareen , Singh Thakur Gurjeet , Singh Manjinder , Najda Agnieszka , Nurzyńska-Wierdak Renata , Almeer Rafa , Kamel Mohamed , Abdel-Daim Mohamed M.
Published: November 2021

Chondroitin sulfate is a proteoglycan component of the extracellular matrix (ECM) that supports neuronal and non-neuronal cell activity, provides a negative domain to the extracellular matrix, regulates the intracellular positive ion concentration, and maintains the hypersynchronous epileptiform activity. Therefore, the present study hypothesized an antiepileptic potential of chondroitin sulfate (CS) in pentylenetetrazole-induced kindled epilepsy and pilocarpine-induced status epilepticus in mice. Levels of various oxidative stress markers and inflammatory mediators were estimated in the brain tissue homogenate of mice, and histopathological changes were evaluated. Treatment with valproate (110 mg/kg; i.p.) as a standard drug and chondroitin sulfate (100 & 200 mg/kg, p.o.) significantly (p < 0.01) and dose-dependently prevented the severity of kindled and spontaneous recurrent seizures in mice. Additionally, chondroitin sulfate showed its antioxidant potential by restoring the various biochemical levels and anti-inflammatory properties by reducing NF-kB levels and pro-inflammatory mediators like TNF-alpha, IL-1β, and IL-6, indicating the neuroprotective effect as well as the suppressed levels of caspase-3, which indicated a neuroprotective treatment strategy in epilepsy. The proteoglycan chondroitin sulfate restores the normal physiology and configuration of the neuronal tissue. Further, the molecular docking of chondroitin sulfate at the active pockets of TNF-alpha, IL-1β, and IL-6 showed excellent interactions with critical amino acid residues. In conclusion, the present work provides preclinical evidence of chondroitin sulfate as a new therapeutic approach in attenuating and preventing seizures with a better understanding of the mechanism of alteration in ECM changes influencing abnormal neuronal activities.
Thrombolytic Agents: Nanocarriers in Targeted Release
Authors: Shen Minghua , Wang Yujiao , Hu Fan , Lv Linwen , Chen Kui , Xing Gengmei
Published: November 2021

A thrombus, known as a blood clot, may form within the vascular system of the body and impede blood flow. Thrombosis is the most common underlying pathology of cardiovascular diseases, contributing to high morbidity and mortality. However, the main thrombolytic drugs (urokinase, streptokinase, etc.) have shortcomings, including a short half-life, serious side effects and a lack of targeting, that limit their clinical application. The use of nano-drug delivery systems is expected to address these problems and a variety of approaches, including biological and physical responsive systems, have been explored. In this report, recent advances in the development of targeted nano-drug delivery systems are thoroughly reviewed.