bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
Authors: Majorova Dominika , Atkins Elizabeth , Martineau Henny , Vokral Ivan , Oosterhuis Dorenda , Olinga Peter , Wren Brendan , Cuccui Jon , Werling Dirk
Published: June 2021

The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue models that can more closely recapitulate the host tissue during disease and repair. Whereas organoids and organs-on-a-chip technologies have their benefits, they still cannot provide the cellular and structural complexity of the host tissue. Here, precision cut tissue slices (PCTS) may provide invaluable models for complex ex-vivo generated tissues to assess host-pathogen interaction as well as potential vaccine responses in a “whole organ” manner. In this mini review, we discuss the current literature regarding PCTS in veterinary species and advocate that PCTS represent remarkable tools to further close the gap between target identification, subsequent translation of results into clinical studies, and thus opening avenues for future precision medicine approaches.
Optimization of Profit for Pasture-Based Beef Cattle and Sheep Farming Using Linear Programming: Model Development and Evaluation
Authors: Addis Addisu H. , Blair Hugh T. , Kenyon Paul R. , Morris Stephen T. , Schreurs Nicola M.
Published: June 2021

A linear programming optimization tool is useful to assist farmers with optimizing resource allocation and profitability. This study developed a linear programming profit optimization model with a silage supplement scenario. Utilizable kilograms of pasture dry matter (kg DM) of the total pasture mass was derived using minimum and maximum pasture mass available for beef cattle and sheep and herbage utilization percentage. Daily metabolizable energy (MJ ME/head) requirements for the various activities of beef cattle and sheep were estimated and then converted to kg DM/head on a bi-monthly basis. Linear programming was employed to identify the optimum carrying capacity of beef cattle and sheep, the most profitable slaughtering ages of beef cattle, the number of prime lambs (sold to meat processing plants), and sold store lambs (sold to other farmers for finishing). Gross farm revenue (GFR) and farm earnings before tax (EBT) per hectare and per stock unit, as well as total farm expenditure (TFE), were calculated and compared to the average value of Taranaki-Manawatu North Island intensive finishing sheep and beef Class 5 farming using Beef and Lamb New Zealand (B+LNZ) data. The modeled farm ran 46% more stock units (a stock unit consumed 550 kg DM/year) than the average value of Class 5 farms. At this stocking rate, 83% of the total feed supplied for each species was consumed, and pasture supplied 95% and 98% of beef cattle and sheep feed demands, respectively. More than 70% of beef cattle were finished before the second winter. This enabled the optimized system to return 53% and 188% higher GFR/ha and EBT/ha, respectively, compared to the average values for a Class 5 farm. This paper did not address risk, such as pasture growth and price fluctuations. To understand this, several additional scenarios could be examined using this model. Further studies to include alternative herbages and crops for feed supply during summer and winter are required to expand the applicability of the model for different sheep and beef cattle farm systems.
Differential Morphophysiological, Biochemical, and Molecular Responses of Maize Hybrids to Salinity and Alkalinity Stresses
Authors: Fatima Arooj , Hussain Saddam , Hussain Sadam , Ali Basharat , Ashraf Umair , Zulfiqar Usman , Aslam Zubair , Al-Robai Sami Asir , Alzahrani Fatima Omari , Hano Christophe , El-Esawi Mohamed A.
Published: June 2021

Salinity and alkalinity stresses are common in arid and semiarid climates. Both these stresses not only retard crop growth but also cause a severe reduction in yields. The present experiment was performed to investigate the morphological, physiological, biochemical, and genetic responses of two maize hybrids (FH-1231 and DK-6714) to salinity and alkalinity stresses. The treatments were comprised of salt stress (NaCl:Na2SO4 at a 9:1 ratio), alkaline stress (NaHCO3:Na2CO3 at a 9:1 ratio), and an unstressed control. The results indicated that salinity and alkalinity significantly reduced shoot fresh weight by 50% and 70%, root fresh weight by 38% and 50%, root dry weight by 69% and 93%, seedling length by 18% and 30%, number of leaves by 27% and 39%, and maximum leaf width by 17% and 24%, respectively, across the two hybrids compared with control, indicating that alkalinity had a greater effect than salinity. Likewise, both the stresses, particularly alkalinity, significantly decreased K+ ion accumulation and chlorophyll content and increased the lipid peroxidation rate, sodium (Na+) concentration, the hydrogen peroxide (H2O2) level, and the activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). Salinity and alkalinity stresses also induced the expression levels of antioxidant genes (SOD, CAT, POD, APX); however, salinity showed less effect than alkalinity stress. Similarly, hybrid DK-6714 performed comparatively better than FH-1231 with regard to seedling growth, antioxidant activities, and biochemical attributes under stress conditions. Thus, DK-6714 is recommended as a suitable hybrid for soils affected with salt-alkalization.
A Future-Focused View of the Regulation of Rural Technology
Authors: Martin Paul
Published: June 2021

There has been an explosion of innovation in agricultural technologies, but whether the anticipated benefits are fully realised depends partly upon with the institutional structures are supportive. Many types of law will shape what innovations are viable and the scale of the economic returns. Australia does not have a coherent strategy for future rural regulation that will both minimise the public risks and increase the private opportunities from future agricultural innovation. This paper addresses these issues. It considers the diverse legal issues that will affect these opportunities, and it looks particularly at agricultural robotics as an example of the many ways in which legal issues will shape opportunities from innovation. It proposes that an integrated strategy, based on a careful analysis of future issues, would be a significant contributor to Australia’s agricultural sector and to its innovating industries.
Selenium Biofortification of Three Wild Species, Rumex acetosa L., Plantago coronopus L., and Portulaca oleracea L., Grown as Microgreens
Authors: Puccinelli Martina , Pezzarossa Beatrice , Pintimalli Lucia , Malorgio Fernando
Published: June 2021

Microgreens of wild herbs are a source of healthy compounds. Selenium (Se) biofortification of microgreens could help increase the Se content and thus contribute to Se requirements in humans. We evaluated whether three wild herbs, Rumex acetosa L., Plantago coronopus L., and Portulaca oleracea L., were suitable for biofortification in order to obtain products with high nutraceutical value. In the first experiment, the three species were enriched with Na2SeO4 at 0 and 1.5 mg Se L−1, and the effects of Se on the nutraceutical characteristics of microgreens were evaluated. In the second experiment, using P. oleracea enriched with 0, 1.5, 5, and 10 mg Se L−1, we investigated whether there was a relation between the increasing Se concentrations in the nutrient solution and the Se content in microgreens. The Se added was taken up by roots and accumulated in the aerial part. P. coronopus exhibited the highest ability to accumulate selenium, and the Se-enriched microgreens showed the highest chlorophyll and flavonoid content. The strong correlation between the Se concentration in the growth solution and the Se accumulated in P. oleracea may enable the cultivation of microgreens with the targeted Se content. The resulting Se-biofortified microgreens of wild species could represent a new vegetable product with high nutraceutical value also ensuring a sufficient dietary intake of Se.
Simplified and Advanced Sentinel-2-Based Precision Nitrogen Management of Wheat
Authors: Santaga Francesco Saverio , Benincasa Paolo , Toscano Piero , Antognelli Sara , Ranieri Emanuele , Vizzari Marco
Published: June 2021

This study compares simplified and advanced precision nitrogen (N) fertilization approaches for winter wheat relying on Sentinel-2 NDVI, grain yield maps, and protein content. Five N fertilization treatments were compared: (1) a standard rate, calculated by a typical N balance (Flat-N); (2) a variable rate calculated using a simplified linear model, adopting a proportional strategy (NDVI directly related) (Var-N-dir); (3) a variable rate calculated using a simplified linear model, adopting a compensative strategy (NDVI inversely related) (Var-N-inv); (4) a variable rate calculated using the AgroSat model (Var-N-Agrosat); and (5) a variable rate calculated applying the Agricolus model (Var-N-Agricolus). The study was carried out in four fields over two cropping seasons with a randomized blocks design. Results indicate that the weather remains the main factor influencing yield, as it typically happens in a rainfed crop. No substantial differences in crop yield were observed among the N fertilization models within each year and experimental location. However, in the more favorable season, the low-input direct model (Var-N-dir) resulted as the best choice, providing the higher NUE (nitrogen use efficiency) value. In the less favorable season, results showed a better performance of the advanced models (Var-N-Agricolus and Var-N-Agrosat), which limited yield losses and reduced intra-field variability, with relevant importance given to the increasing frequency of abnormal climate phenomena. In general, all these VRT approaches allowed reduction of the excess of fertilizers, preservation of the environment, and saving money.
Effects of Different Non-Cage Housing Systems on the Production Performance, Serum Parameters and Intestinal Morphology of Laying Hens
Authors: Wan Yi , Yang Huan , Zhang Hongyi , Ma Ruiyu , Qi Renrong , Li Junying , Liu Wei , Li Yan , Zhan Kai
Published: June 2021

This study investigated the effects of plastic-net housing system (NRS) and floor-litter housing system (LRS) on the production performance, serum parameters and intestinal morphology of Shendan laying hens. A total of 1200 30-week-old hens were randomly allocated to the NRS and LRS groups, each of which included five replicates with 120 chickens in each replicate. The experiment was conducted from 32 to 40 weeks of age. Indoor airborne parameters were measured every 2 weeks, and indoor ground contamination was measured monthly. The laying rate and mortality of hens were recorded daily, and egg quality traits and serum parameters were measured every 2 weeks. At 40 weeks of age, four birds per replicate from each experimental group were selected for intestinal morphological observation. The results showed that the airborne bacteria number in the LRS was significantly higher than that in the NRS (p < 0.05) for most of the experimental period (except at 32 and 38 weeks of age), and the bacterial numbers on the surfaces of the floor and floor eggs in the LRS were approximately 10 times higher than those in the NRS (p < 0.05). Compared with the LRS, the NRS improved the laying rate (p < 0.05), reduced serum malondialdehyde (MDA) (p < 0.05) and corticosterone (CORT) concentrations and increased serum glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, indicating favourable effects on antioxidative status. The NRS was significantly associated with an increased villus height (VH), villus height to crypt depth ratio (VCR) in the small intestine (p < 0.05) and increased VCR in the caecum (p < 0.05). Overall, the lower rate of bacterial contamination in the NRS than in the LRS indicated better environmental hygiene. The NRS enhanced the laying performance and antioxidant capacity of hens and was superior to the LRS in improving intestinal health. The current findings support the advantages of the NRS for the health and welfare of Shendan chickens during the peak laying period.
Variations in Behavioral and Physiological Traits in Yearling Tibetan Sheep (Ovis aries)
Authors: Yu Yibo , Wang Yun , Zhong Liang , Zhu Hongjuan , Qu Jiapeng
Published: June 2021

Temperament is a consistent behavioral difference among individuals over time or in different contexts. A comprehensive understanding of temperament and complex behavioral interactions enhances knowledge on animal evolution, welfare, and productivity. However, reports on the development of behavioral consistency over ontogeny are vague. Here, we tested the ontogeny of the temperament and physiological traits of Tibetan sheep (Ovis aries) in three crucial age stages. The mean level of the risk-taking variable increased, while that of the vocalizations variable decreased. The exploration variable was stable over ontogeny. The novelty decreased and the heart rate increased from the juvenile to the adolescent stage but stabilized at the adult stage. The fecal cortisol concentration (CORT) variable was stable at the juvenile and adolescent stages but decreased at the adult stage. Stable correlations were reported for the juvenile and adolescent stages and for the behavioral variables and heart rate. However, some correlations emerged only after maturation, whereas others disappeared over ontogeny. Moreover, CORT was independent of temperament and heart rate at different ages. These results demonstrate that age affects temperament and physiology and their correlations. Hence, developmental aspects should be incorporated into future temperament studies.
Effect of Selenium Sources on Laying Performance, Egg Quality Characteristics, Intestinal Morphology, Microbial Population and Digesta Volatile Fatty Acids in Laying Hens
Authors: Muhammad Aliyu Ibrahim , Mohamed Dalia Alla , Chwen Loh Teck , Akit Henny , Samsudin Anjas Asmara
Published: June 2021

The use of toxic and less bioavailable inorganic selenium can now be supplemented with an alternative organic source from bacterial species in nutrition for human and animal benefit. This study investigated the effects of selenium sources on laying performance, egg quality characteristics, intestinal morphology, caecum microbial population, and digesta volatile fatty acids in laying hens. One hundred and forty-four Lohman Brown Classic laying hens, at 23 weeks of age, were divided into four experimental groups (36 hens in each), differing in form of Se supplementation: no Se supplementation (Con), 0.3 mg/kg of inorganic Se in the form of sodium selenite (Na2SeO3), 0.3 mg/kg of organic Se from selenium yeast (Se-Yeast), and 0.3 mg/kg of organic Se from Stenotrophomonas maltophilia (bacterial organic Se, ADS18). The results showed that different dietary Se sources significantly affected laying rate, average egg weight, daily egg mass, feed conversion ratio (FCR), and live bodyweight (LBW) (p < 0.05). However, average daily feed intake and shell-less and broken eggs were unaffected (p > 0.05) among the treatment groups. The findings revealed that selenium sources had no (p > 0.05) effect on egg quality (external and internal) parameters. However, eggshell breaking strength and Haugh unit were significantly (p < 0.05) improved with organic (ADS18 or Se-yeast) Se-fed hens compared to the control group. In addition, egg yolk and breast tissue Se concentrations were higher (p < 0.05) in the dietary Se supplemented group compared to the control. Intestinal histomorphology revealed that hens fed ADS18 or Se-Yeast groups had significantly (p < 0.05) higher villi height in the duodenum and jejunum compared to those fed Na2SeO3 or a basal diet. However, when compared to organic Se fed (ADS18 or Se-Yeast) hens, the ileum villus height was higher (p < 0.05) in the basal diet group; with the lowest in the SS among the treatment groups. A significant increase (p < 0.05) of Lactobacilli spp. and Bifidobacteria spp., and a decrease of Escherichia coli and Salmonella spp. population were observed in the organic (ADS18 or Se-yeast) compared to inorganic supplemented and control hens. The individual digesta volatile fatty acid (VFA) was significantly different, but with no total VFA differences. Thus, bacterial selenoprotein or Se-yeast improved the performance index, egg quality characteristics, egg yolk and tissue Se contents, and intestinal villus height in laying hens. Moreover, caecum beneficial microbes increased with a decrease in the harmful microbe population and affected individual cecal volatile fatty acids without affecting the total VFA of the laying hens digesta.
Etiology and Antimicrobial Susceptibility of Pathogens Associated with Urinary Tract Infections among Women Attending Antenatal Care in Four South African Tertiary-Level Facilities, 2015–2019
Authors: Zwane Thembekile , Shuping Liliwe , Perovic Olga
Published: June 2021

In South Africa, uncomplicated community-acquired UTIs (CA-UTIs) are treated empirically; however, the extent of antibiotic resistance among these pathogens is not well known. We conducted a descriptive cross-sectional study of women attending ANCs at four tertiary public-sector hospitals in Gauteng. Female patients aged 15–49 years, with urine cultures performed between January 2015 and December 2019, were included. A case of culture-confirmed UTI was defined as any woman with ≤2 uropathogens with a bacterial count of ≥105 colony-forming units per ml for at least one pathogen. We identified 3558 cases of culture-confirmed UTIs in women with a median age of 30 years (interquartile range; 25–35). E. coli accounted for most infections (56% (1994/3558)), followed by E. faecalis, with a prevalence of 17% (609/3558). The prevalence of K. pneumoniae was 5% (193/3558), 5% (186/3558) for S. agalactiae, and 5% (179/3558) for P. mirabilis. Ninety-five percent (1827/1927) of the E. coli and 99% of the E. faecalis (301/305) isolates were susceptible to nitrofurantoin. Common uropathogens showed high susceptibility to first-line antibiotics, gentamicin and nitrofurantoin, as recommended for use in primary healthcare settings. Overall, our study provided an indication of the level of antimicrobial resistance in the four facilities.
First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing E. coli Isolates from Diarrheic Poultry in Tunisia
Authors: Jouini Ahlem , Klibi Amira , Elarbi Imen , Chaabene Meriem Ben , Hamrouni Safa , Souiai Oussema , Hanachi Mariem , Ghram Abdeljelil , Maaroufi Abderrazak
Published: June 2021

Circulation of a multi-resistance clone of bacteria associated with genetic elements in diseased animals constitutes a global public health problem. Our study focused on the characterization of the support of ESBL in cefotaxime resistant E. coli (CTXR) isolates recovered from poultry with diarrhea, analysis of their clonal lineage, and virulence-associated genes. The study was carried out on 130 samples of chickens with diarrhea, collected in 2015 from poultry farms in Tunisia. Isolates of 20 CTXR E. coli strains were identified as ESBL and AmpC β- lactamase producers. The following β-lactamase genes (number of isolates) were detected: blaCTX-M-15+ blaOXA1 (4), blaCTX-M-15 + blaOXA1 + blaTEM-1b (2), blaCTX-M-1 + blaTEM-1b (9), blaCTX-M-1 (2), blaCMY2 + blaTEM-1b (3). Six E. coli harboring blaCTXM-15 were allocated to ST131-B2-O25b-; six and three blaCTX-M-1 were grouped in ST155, ST10, and ST58, respectively, related to the phylogroup D and A. The qnrB gene, the variant aac(6′)-Ib-cr, and the class 1 integrons with different gene cassettes, were detected amongst our 20 isolated strains, which were classified as ExPEC and aEPEC. Our findings highlighted the emergence of the human pandemic ST131-CTX-M-15-O25-B2 clone and the high risk of such clonal lineage strains in diarrheic poultry, in Tunisia, which could constitute a risk of their transfer to healthy animals and humans.
Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes
Authors: Đurašević Siniša , Pejić Snežana , Grigorov Ilijana , Nikolić Gorana , Mitić-Ćulafić Dragana , Dragićević Milan , Đorđević Jelena , Todorović Vukotić Nevena , Đorđević Neda , Todorović Ana , Drakulić Dunja , Veljković Filip , Pajović Snežana B. , Todorović Zoran
Published: June 2021

Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.
A Review of Thermal Error Modeling Methods for Machine Tools
Authors: Li Yang , Yu Maolin , Bai Yinming , Hou Zhaoyang , Wu Wenwu
Published: June 2021

Thermal error caused by thermal deformation is one of the most significant factors influencing the accuracy of the machine tool. Compensation is a practical and efficient method to reduce the thermal error. Among all the thermal error compensation processes, thermal error modeling is the premise and basis because the effectiveness of the compensation is directly determined by the accuracy and robustness of modeling. In this paper, an overview of the thermal error modeling methods that have been researched and applied in the past ten years is presented. First, the modeling principle and compensation methods of machine tools are introduced. Then, the methods are classified and summarized in detail. Finally, the future research trend of thermal error modeling is forecasted.
The Optimization Analysis for the Original and Manipulation Identification of Illegally Filmed Images
Authors: Choi Soohyeon , Kim Dohoon
Published: June 2021

Illegally filmed images, the sharing of non-consensually filmed images over social media, and the secret recording and distribution of celebrity images are increasing. To catch distributors of illegally filmed images, many investigation techniques based on an analysis of the file attribute information of the original images have been introduced. As forensic science advances, various types of anti-forensic technologies are being produced, requiring investigators to open and analyze all videos from the suspect’s storage devices, raising the question of the invasion of privacy during the investigation. The suspect can even file a lawsuit, which makes issuing a warrant and conducting an investigation difficult. Thus, it is necessary to detect the original and manipulated images without needing to directly go through multiple videos. We propose an optimization analysis and detection method for extracting original and manipulated images from seized devices of suspects. In addition, to increase the detection rate of both original and manipulated images, we suggest a precise measurement approach for comparative thresholds. Thus, the proposed method is a new digital forensic methodology for comparing and identifying original and manipulated images accurately without the need for opening videos individually in a suspect’s mobile device.
Evaluation of Carbon Fiber Grid Reinforced Concrete Panel for Disaster Response and Improved Seismic Performance
Authors: Kang Sukpyo , Chai Charles , Hong Seonguk
Published: June 2021

The purpose of this study is to enable emergency recovery of damage caused by earthquakes in structures and prevent secondary damage by controlling progressive collapse. Although there have been many previous studies using reinforcing bars and meshes, there have been few studies using carbon grid fibers as a substitute for tension members. This study aims to quantitatively present disaster response seismic performance by manufacturing concrete panels with no layers, one layer, or two layers of carbon fiber grids inserted and to compare the flexural strength and energy dissipation capacity. Flexural strength increased by 7% with one layer and 15% with two layers compared with no layer. Energy dissipation capacity increased by 30 times with one layer and by 56 times with two layers compared with no layers, and showed great improvement in terms of seismic performance. Especially because of the large increase in the energy dissipation capacity, the carbon fiber grid reinforcement method is considered to be an effective method for improving seismic performance.
An Efficient System for Automatic Blood Type Determination Based on Image Matching Techniques
Authors: Odeh Nuha , Toma Anas , Mohammed Falah , Dama Yousef , Oshaibi Farah , Shaar Muna
Published: June 2021

This paper presents a fast and accurate system to determine the type of blood automatically based on image processing. Blood type determination is important in emergency situations, where there is a need for blood transfusion to save lives. The traditional blood determination techniques are performed manually by a specialist in medical labs, where the result requires a long time or may be affected by human error. This may cause serious consequences or even endanger people’s lives. The proposed approach performs blood determination in real-time with low cost using any available mobile device equipped with a camera. A total of 500 blood samples were processed in this study using different image matching techniques including oriented fast and rotated brief (ORB), scale invariant feature transform (SIFT), and speed-up robust feature (SURF). The evaluation results show that our proposed system, which adopts the ORB algorithm, is the fastest and the most accurate among the state-of-the-art systems. It can achieve an accuracy of 99.6% in an average time of 250 ms.
Development of Decision-Making Factors to Determine EMP Protection Level: A Case Study of a Brigade-Level EMP Protection Facility
Authors: Kim Kukjoo , Park Young-Jun
Published: June 2021

This study developed decision-making factors to classify electromagnetic pulse (EMP) protection levels and determine various protection measures. We proposed three EMP protection levels of 80, 60, and 40 dB by considering the characteristics of military equipment and factors that determine EMP protection level, based on a Delphi study. We modeled EMP protection facilities for brigade-level troops to evaluate the derived decision-making factors and applicability of differential protection levels. The natural attenuation effect of soil was confirmed for structures installed underground. The shielding effect of wet soil was up to 30 dB. Considering the 20 dB EMP resistance of military equipment and the 30 dB of attenuation of wet soil, new materials with 30 dB of shielding efficiency could be used to meet an EMP protection level of 80 dB. Therefore, we confirmed that EMP protection measures could be established to build mobile, lightweight shelters. If lightweight shelters are constructed by applying the differential protection level scheme, they can be applied as a more effective EMP protection measure. Furthermore, differential protection measures can be adopted as a sustainable defense facility policy approach, wherein lightweight protection facilities replace conventional heavyweight facilities.
Small Heat Source Used for Combustion of Wheat-Straw Pellets
Authors: Pafcuga Marian , Holubcik Michal , Durcansky Peter , Kapjor Andrej , Malcho Milan
Published: June 2021

Wheat straw, as a secondary waste agricultural product, presents a potential renewable source of energy. It is necessary to design simple heat sources to achieve better usage. As part of an analysis of heat sources, a tubular type of heat exchanger was reviewed. The design of the exchanger was focused on the smallest possible dimensional requirements, low costs, high reliability and easy maintenance. We chose a tubular type of heat exchanger. In our case, flue gas flows through the tubes, and water flows outside of the tubes. This type of exchanger allows for continuous cleaning, but also simple maintenance, even in case of equipment failure. It is possible to replace individual parts (pipes) of equipment that are exposed to the adverse corrosive effects of biomass flue gases. A mathematical model was composed to compute the construction of a heat source. The model was verified by CFD simulation. The main idea of this design is modularity. The composed model can be used to design a series of similar heat sources with different levels of power, and which, as it is of maximal availability for this type of construction, increase use of waste straw as fuel by small farms.
Polysaccharides and Derivatives from Africa to Address and Advance Sustainable Development and Economic Growth in the Next Decade
Authors: Sarraf Antony , Verton Emeline , Addoun Noura , Boual Zakaria , Ould El Hadj Mohamed Didi , El Alaoui-Talibi Zainab , El Modafar Cherkaoui , Abdelkafi Slim , Fendri Imen , Delattre Cédric , Dubessay Pascal , Michaud Philippe , Pierre Guillaume
Published: June 2021

Polysaccharides are highly variable and complex biomolecules whose inventory of structures is still very incomplete, as nature still preserves unexplored biotopes. Plants, macroalgae and microalgae are an integral part of the daily life of human being regardless of culture, time, or knowledge development of a country. Natural medicine is an ancestral knowledge widely distributed throughout the world, handed down for centuries from generation to generation by those commonly referred to as “nganga” healers or shamans. It is also called alternative medicine or traditional medicine, and has been associated for millennia to legends. This review gives an emphasis regarding the ethnobotanic approach associated to the structural variability of poly- and oligosaccharides for designing the new polysaccharide-based drugs and hydrocolloids of tomorrow. The guiding thread is to survey the potential of plants (and some macroalgae) from Africa as a source of polysaccharides with original structures and, secondly, to correlate these structures with biological and/or functional properties in particular to address and advance the sustainable development and economic growth of mankind.
HP-SFC: Hybrid Protection Mechanism Using Source Routing for Service Function Chaining
Authors: Raza Syed M. , Jeong Haekwon , Kim Moonseong , Choo Hyunseung
Published: June 2021

Service Function Chaining (SFC) is an emerging paradigm aiming to provide flexible service deployment, lifecycle management, and scaling in a micro-service architecture. SFC is defined as a logically connected list of ordered Service Functions (SFs) that require high availability to maintain user experience. The SFC protection mechanism is one way to ensure high availability, and it is achieved by proactively deploying backup SFs and installing backup paths in the network. Recent studies focused on ensuring the availability of backup SFs, but overlooked SFC unavailability due to network failures. This paper extends our previous work to propose a Hybrid Protection mechanism for SFC (HP-SFC) that divides SFC into segments and combines the merits of local and global failure recovery approaches to define an installation policy for backup paths. A novel labeling technique labels SFs instead of SFC, and they are stacked as per the order of SFs in a particular SFC before being inserted into a packet header for traffic steering through segment routing. The emulation results showed that HP-SFC recovered SFC from failure within 20–25 ms depending on the topology and reduced backup paths’ flow entries by at least 8.9% and 64.5% at most. Moreover, the results confirmed that the segmentation approach made HP-SFC less susceptible to changes in network topology than other protection schemes.
Erratum: Hůnová, I. Ambient Air Quality in the Czech Republic: Past and Present. Atmosphere 2020, 11, 214
Authors: Hůnová Iva
Published: June 2021

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A Decision-Making Approach Based on New Aggregation Operators under Fermatean Fuzzy Linguistic Information Environment
Authors: Verma Rajkumar
Published: June 2021

Fermatean fuzzy linguistic (FFL) set theory provides an efficient tool for modeling a higher level of uncertain and imprecise information, which cannot be represented using intuitionistic fuzzy linguistic (IFL)/Pythagorean fuzzy linguistic (PFL) sets. On the other hand, the linguistic scale function (LSF) is the better way to consider the semantics of the linguistic terms during the evaluation process. It is worth noting that the existing operational laws and aggregation operators (AOs) for Fermatean fuzzy linguistic numbers (FFLNs) are not valid in many situations, which can generate errors in real-life applications. The present study aims to define new robust operational laws and AOs under Fermatean fuzzy linguistic environment. To do so, first, we define some new modified operational laws for FFLNs based on LSF and prove some important mathematical properties of them. Next, the work defines several new AOs, namely, the FFL-weighted averaging (FFLWA) operator, the FFL-weighted geometric (FFLWG) operator, the FFL-ordered weighted averaging (FFLOWA) operator, the FFL-ordered weighted geometric (FFLOWG) operator, the FFL-hybrid averaging (FFLHA) operator and the FFL-hybrid geometric (FFLHG) operator under Fermatean fuzzy linguistic environment. Several properties of these AOs are investigated in detail. Further, based on the proposed AOs, a new decision-making approach with Fermatean fuzzy linguistic information is developed to solve group decision-making problems with multiple attributes. Finally, to illustrate the effectiveness of the present approach, a real-life supplier selection problem is presented where the evaluation information of the alternatives is given in terms of FFLNs. Compared to the existing methods, the salient features of the developed approach are (1) it can solve decision-making problems with qualitative information data using FFLNs; (2) It can consider the attitudinal character of the decision-makers during the solution process; (3) It has a solid ability to distinguish the optimal alternative.
Rethinking a Non-Predominant Pattern in Invasive Lung Adenocarcinoma: Prognostic Dissection Focusing on a High-Grade Pattern
Authors: Choi Yeonu , Kim Jonghoon , Park Hyunjin , Kim Hong Kwan , Kim Jhingook , Jeong Ji Yun , Ahn Joong Hyun , Lee Ho Yun
Published: June 2021

Background: Prognostic considerations for non-predominant patterns are necessary because most lung adenocarcinomas (ADCs) have a mixed histologic pattern, and the spectrum of actual prognosis varies widely even among lung ADCs with the same most predominant pattern. We aimed to identify prognostic stratification by second most predominant pattern of lung ADC and to more accurately assess prognostic factors with CT imaging analysis, particularly enhancing non-predominant but high-grade pattern. Methods: In this prospective study, patients with early-stage lung ADC undergoing curative surgery underwent preoperative dual-energy CT (DECT) and positron emission tomography (PET)/CT. Histopathology of ADC, the most predominant and second most predominant histologic patterns, and preoperative imaging parameters were assessed and correlated with patient survival. Results: Among the 290 lung ADCs included in the study, 231 (79.7%) were mixed-pathologic pattern. When the most predominant histologic pattern was intermediate-grade, survival curves were significantly different among the three second most predominant subgroups (p = 0.004; low, lepidic; intermediate, acinar and papillary; high, micropapillary and solid). When the second most predominant pattern was high-grade, recurrence risk increased by 4.2-fold compared with the low-grade group (p = 0.005). To predict a non-predominant but high-grade pattern, the non-contrast CT value of tumor was meaningful with a lower HU value associated with the histologic combination of lower grade (low-grade as most predominant and intermediate-grade as second most predominant pattern, OR = 6.15, p = 0.005; intermediate-grade as most predominant and high-grade as second most predominant pattern, OR = 0.10, p = 0.033). SUVmax of the tumor was associated with the non-predominant but high-grade pattern, especially in the histologic combination of intermediate-high grade (OR = 1.14, p = 0.012). Conclusions: The second most predominant histologic pattern can stratify lung ADC patients according to prognosis. Thus, predicting the malignant potential and establishing treatment policies should not rely only on the most predominant pattern. Moreover, imaging parameters of non-contrast CT value and SUVmax could be useful in predicting a non-predominant but high-grade histologic pattern.
Approaches to Enhance Natural Killer Cell-Based Immunotherapy for Pediatric Solid Tumors
Authors: Quamine Aicha E. , Olsen Mallery R. , Cho Monica M. , Capitini Christian M.
Published: June 2021

Treatment of metastatic pediatric solid tumors remain a significant challenge, particularly in relapsed and refractory settings. Standard treatment has included surgical resection, radiation, chemotherapy, and, in the case of neuroblastoma, immunotherapy. Despite such intensive therapy, cancer recurrence is common, and most tumors become refractory to prior therapy, leaving patients with few conventional treatment options. Natural killer (NK) cells are non-major histocompatibility complex (MHC)-restricted lymphocytes that boast several complex killing mechanisms but at an added advantage of not causing graft-versus-host disease, making use of allogeneic NK cells a potential therapeutic option. On top of their killing capacity, NK cells also produce several cytokines and growth factors that act as key regulators of the adaptive immune system, positioning themselves as ideal effector cells for stimulating heavily pretreated immune systems. Despite this promise, clinical efficacy of adoptive NK cell therapy to date has been inconsistent, prompting a detailed understanding of the biological pathways within NK cells that can be leveraged to develop “next generation” NK cell therapies. Here, we review advances in current approaches to optimizing the NK cell antitumor response including combination with other immunotherapies, cytokines, checkpoint inhibition, and engineering NK cells with chimeric antigen receptors (CARs) for the treatment of pediatric solid tumors.
Engineering Calreticulin-Targeting Monobodies to Detect Immunogenic Cell Death in Cancer Chemotherapy
Authors: Zhang Ying , Thangam Ramar , You Sung-Hwan , Sultonova Rukhsora D. , Venu Akhil , Min Jung-Joon , Hong Yeongjin
Published: June 2021

Surface-exposed calreticulin (ecto-CRT) plays a crucial role in the phagocytic removal of apoptotic cells during immunotherapy. Ecto-CRT is an immunogenic signal induced in response to treatment with chemotherapeutic agents such as doxorubicin (DOX) and mitoxantrone (MTX), and two peptides (KLGFFKR (Integrin-α) and GQPMYGQPMY (CRT binding peptide 1, Hep-I)) are known to specifically bind CRT. To engineer CRT-specific monobodies as agents to detect immunogenic cell death (ICD), we fused these peptide sequences at the binding loops (BC and FG) of human fibronectin domain III (FN3). CRT-specific monobodies were purified from E. coli by affinity chromatography. Using these monobodies, ecto-CRT was evaluated in vitro, in cultured cancer cell lines (CT-26, MC-38, HeLa, and MDA-MB-231), or in mice after anticancer drug treatment. Monobodies with both peptide sequences (CRT3 and CRT4) showed higher binding to ecto-CRT than those with a single peptide sequence. The binding affinity of the Rluc8 fusion protein–engineered monobodies (CRT3-Rluc8 and CRT4-Rluc8) to CRT was about 8 nM, and the half-life in serum and tumor tissue was about 12 h. By flow cytometry and confocal immunofluorescence of cancer cell lines, and by in vivo optical bioluminescence imaging of tumor-bearing mice, CRT3-Rluc8 and CRT4-Rluc8 bound specifically to ecto-CRT and effectively detected pre-apoptotic cells after treatment with ICD-inducing agents (DOX and MTX) but not a non-ICD-inducing agent (gemcitabine). Using CRT-specific monobodies, it is possible to detect ecto-CRT induction in cancer cells in response to drug exposure. This technique may be used to predict the therapeutic efficiency of chemo- and immuno-therapeutics early during anticancer treatment.
Motor Evoked Potential Warning Criteria in Supratentorial Surgery: A Scoping Review
Authors: Asimakidou Evridiki , Abut Pablo Alvarez , Raabe Andreas , Seidel Kathleen
Published: June 2021

During intraoperative monitoring of motor evoked potentials (MEP), heterogeneity across studies in terms of study populations, intraoperative settings, applied warning criteria, and outcome reporting exists. A scoping review of MEP warning criteria in supratentorial surgery was conducted in accordance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). Sixty-eight studies fulfilled the eligibility criteria. The most commonly used alarm criteria were MEP signal loss, which was always a major warning sign, followed by amplitude reduction and threshold elevation. Irreversible MEP alterations were associated with a higher number of transient and persisting motor deficits compared with the reversible changes. In almost all studies, specificity and Negative Predictive Value (NPV) were high, while in most of them, sensitivity and Positive Predictive Value (PPV) were rather low or modest. Thus, the absence of an irreversible alteration may reassure the neurosurgeon that the patient will not suffer a motor deficit in the short-term and long-term follow-up. Further, MEPs perform well as surrogate markers, and reversible MEP deteriorations after successful intervention indicate motor function preservation postoperatively. However, in future studies, a consensus regarding the definitions of MEP alteration, critical duration of alterations, and outcome reporting should be determined.
Molecular Pathogenesis and Regulation of the miR-29-3p-Family: Involvement of ITGA6 and ITGB1 in Intra-Hepatic Cholangiocarcinoma
Authors: Hozaka Yuto , Seki Naohiko , Tanaka Takako , Asai Shunichi , Moriya Shogo , Idichi Tetsuya , Wada Masumi , Tanoue Kiyonori , Kawasaki Yota , Mataki Yuko , Kurahara Hiroshi , Ohtsuka Takao
Published: June 2021

The aggressive nature of intrahepatic cholangiocarcinoma (ICC) renders it a particularly lethal solid tumor. Searching for therapeutic targets for ICC is an essential challenge in the development of an effective treatment strategy. Our previous studies showed that the miR-29-3p-family members (miR-29a-3p, miR-29b-3p and miR-29c-3p) are key tumor-suppressive microRNAs that control many oncogenic genes/pathways in several cancers. In this study, we searched for therapeutic targets for ICC using the miR-29-3p-family as a starting point. Our functional studies of cell proliferation, migration and invasion confirmed that the miR-29-3p-family act as tumor-suppressors in ICC cells. Moreover, in silico analysis revealed that “focal adhesion”, “ECM-receptor”, “endocytosis”, “PI3K-Akt signaling” and “Hippo signaling” were involved in oncogenic pathways in ICC cells. Our analysis focused on the genes for integrin-α6 (ITGA6) and integrin-β1 (ITGB1), which are involved in multiple pathways. Overexpression of ITGA6 and ITGB1 enhanced malignant transformation of ICC cells. Both ITGA6 and ITGB1 were directly regulated by the miR-29-3p-family in ICC cells. Interestingly, expression of ITGA6/ITGB1 was positively controlled by the transcription factor SP1, and SP1 was negatively controlled by the miR-29-3p-family. Downregulation of the miR-29-3p-family enhanced SP1-mediated ITGA6/ITGB1 expression in ICC cells. MicroRNA-based exploration is an attractive strategy for identifying therapeutic targets for ICC.
A Prediction Model for Metachronous Peritoneal Carcinomatosis in Patients with Stage T4 Colon Cancer after Curative Resection
Authors: Tsai Tzong-Yun , You Jeng-Fu , Hsu Yu-Jen , Jhuang Jing-Rong , Chern Yih-Jong , Hung Hsin-Yuan , Yeh Chien-Yuh , Hsieh Pao-Shiu , Chiang Sum-Fu , Lai Cheng-Chou , Chiang Jy-Ming , Tang Reiping , Tsai Wen-Sy
Published: June 2021

(1) Background: The aim of this study was to develop a prediction model for assessing individual mPC risk in patients with pT4 colon cancer. Methods: A total of 2003 patients with pT4 colon cancer undergoing R0 resection were categorized into the training or testing set. Based on the training set, 2044 Cox prediction models were developed. Next, models with the maximal C-index and minimal prediction error were selected. The final model was then validated based on the testing set using a time-dependent area under the curve and Brier score, and a scoring system was developed. Patients were stratified into the high- or low-risk group by their risk score, with the cut-off points determined by a classification and regression tree (CART). (2) Results: The five candidate predictors were tumor location, preoperative carcinoembryonic antigen value, histologic type, T stage and nodal stage. Based on the CART, patients were categorized into the low-risk or high-risk groups. The model has high predictive accuracy (prediction error ≤5%) and good discrimination ability (area under the curve >0.7). (3) Conclusions: The prediction model quantifies individual risk and is feasible for selecting patients with pT4 colon cancer who are at high risk of developing mPC.
Connectivity Map Analysis Indicates PI3K/Akt/mTOR Inhibitors as Potential Anti-Hypoxia Drugs in Neuroblastoma
Authors: Uva Paolo , Bosco Maria Carla , Eva Alessandra , Conte Massimo , Garaventa Alberto , Amoroso Loredana , Cangelosi Davide
Published: June 2021

Neuroblastoma (NB) is one of the deadliest pediatric cancers, accounting for 15% of deaths in childhood. Hypoxia is a condition of low oxygen tension occurring in solid tumors and has an unfavorable prognostic factor for NB. In the present study, we aimed to identify novel promising drugs for NB treatment. Connectivity Map (CMap), an online resource for drug repurposing, was used to identify connections between hypoxia-modulated genes in NB tumors and compounds. Two sets of 34 and 21 genes up- and down-regulated between hypoxic and normoxic primary NB tumors, respectively, were analyzed with CMap. The analysis reported a significant negative connectivity score across nine cell lines for 19 compounds mainly belonging to the class of PI3K/Akt/mTOR inhibitors. The gene expression profiles of NB cells cultured under hypoxic conditions and treated with the mTORC complex inhibitor PP242, referred to as the Mohlin dataset, was used to validate the CMap findings. A heat map representation of hypoxia-modulated genes in the Mohlin dataset and the gene set enrichment analysis (GSEA) showed an opposite regulation of these genes in the set of NB cells treated with the mTORC inhibitor PP242. In conclusion, our analysis identified inhibitors of the PI3K/Akt/mTOR signaling pathway as novel candidate compounds to treat NB patients with hypoxic tumors and a poor prognosis.