bluuebunny/crossref_metadata_2025 huggingface dataset:
Benchmarking of Various Flexible Soft-Computing Strategies for the Accurate Estimation of Wind Turbine Output Power
Authors: Bilal Boudy , Yetilmezsoy Kaan , Ouassaid Mohammed
Published: February 2024

This computational study explores the potential of several soft-computing techniques for wind turbine (WT) output power (kW) estimation based on seven input variables of wind speed (m/s), wind direction (°), air temperature (°C), pitch angle (°), generator temperature (°C), rotating speed of the generator (rpm), and voltage of the network (V). In the present analysis, a nonlinear regression-based model (NRM), three decision tree-based methods (random forest (RF), random tree (RT), and reduced error pruning tree (REPT) models), and multilayer perceptron-based soft-computing approach (artificial neural network (ANN) model) were simultaneously implemented for the first time in the prediction of WT output power (WTOP). To identify the top-performing soft computing technique, the applied models’ predictive success was compared using over 30 distinct statistical goodness-of-fit parameters. The performance assessment indices corroborated the superiority of the RF-based model over other data-intelligent models in predicting WTOP. It was seen from the results that the proposed RF-based model obtained the narrowest uncertainty bands and the lowest quantities of increased uncertainty values across all sets. Although the determination coefficient values of all competitive decision tree-based models were satisfactory, the lower percentile deviations and higher overall accuracy score of the RF-based model indicated its superior performance and higher accuracy over other competitive approaches. The generator’s rotational speed was shown to be the most useful parameter for RF-based model prediction of WTOP, according to a sensitivity study. This study highlighted the significance and capability of the implemented soft-computing strategy for better management and reliable operation of wind farms in wind energy forecasting.
A Fault Location Algorithm for Multi-Section Combined Transmission Lines Considering Unsynchronized Sampling
Authors: Lak Peng Y. , Ha Kwang-Min , Nam Soon-Ryul
Published: February 2024

This paper introduces a fault location technique for multi-section combined transmission lines, utilizing unsynchronized fault record data. The approach determines the intersection points of the voltage magnitudes computed using the fault current and voltage records of each terminal and the constant line parameters of each section. Finally, the real fault location is identified based on the calculated inverse angle of equivalent impedance. To evaluate the performance of the proposed algorithm, a three-section combined transmission line is constructed using the PSCAD/EMTDC software version 5.0.0, and various fault scenarios are simulated and tested employing the proposed algorithm. The results indicate that the proposed algorithm is applicable to various types of faults in a multi-section combined transmission line; the estimations are accurate.
Energy and Economic Sustainability of a Small-Scale Hybrid Renewable Energy System Powered by Biogas, Solar Energy, and Wind
Authors: Figaj Rafał
Published: February 2024

Reduction or elimination of reliance on traditional fossil fuels and of the emission of greenhouse gases and pollutants into the environment are affecting energy technologies, systems, and applications. In this context, one potential approach to achieving sustainability, decarbonization, and ensuring the energy and economic viability of existing and future energy systems involves adopting one or more renewable sources. The presented paper concentrates on examining the performance of a small-scale hybrid renewable polygeneration system. This system utilizes biogas produced through anaerobic digestion, which is then supplied to an internal combustion engine, along with solar energy converted into electrical energy by photovoltaic modules and wind energy harnessed through a wind turbine. A small-scale user, represented by residential buildings and a zootechnical farm with heating, cooling, and electrical energy demands, serves as the case study. TRNSYS software is employed to design and model the system, considering realistic assumptions about technical aspects and user energy requirements. The investigation involves analyzing the system’s operation, considering both energy and economic perspectives. The paper discusses the pros and cons of combining biogas, solar, and wind energy in the proposed hybrid system under the considered case study. Despite non-satisfactory economic profitability without incentives, the proposed system allows one to save significant amounts of primary energy and carbon dioxide equivalent emissions.
Implementation of Positive Energy Districts in European Cities: A Systematic Literature Review to Identify the Effective Integration of the Concept into the Existing Energy Systems
Authors: Clerici Maestosi Paola , Salvia Monica , Pietrapertosa Filomena , Romagnoli Federica , Pirro Michela
Published: February 2024

The positive energy district (PED) is a rather recent concept that aims to contribute to the main objectives of the Energy Union strategy. It is based on an integrated multi-sectoral approach in response to Europe’s most complex challenges. But to what extent have its development and implementation been supported by research and innovation programs? And what is the state of the art of its implementation and effective penetration into the current energy systems of European cities, according to the evidence provided by the scientific literature? This study aims to investigate these issues, providing a critical overview of the PED situation by means of a systematic literature review based on the use of open-access bibliometric software supplemented with content analysis. The results show that less than half of the documents analyzed refer to actual case studies, 80% of which were funded as part of research projects. This seems to lead to the conclusion that although PEDs have been strongly encouraged by the scientific community and policy initiatives at the European level, their implementation in cities is still limited. Moreover, an uneven distribution among countries can be observed. To overcome the existing barriers to PED diffusion and implementation, it would be useful to provide more ad hoc funding and, above all, facilitate its accessibility also by municipalities not yet well integrated into European projects, initiatives, and networks.
Animal Models in Neuroscience: What Is the “Culture of Care”?
Authors: Montanari Martina , Bonsi Paola , Martella Giuseppina , Wirz Annarita
Published: February 2024

In situations where animal models (AMs) are necessary, as in the field of neuroscience, a strong culture of care must be supported and established. The pivotal question remains: how can we uphold a robust “culture of care”? In the multifaceted domain of neuroscience research, AMs traverse a spectrum shaped by conflicting viewpoints, anthropocentrism and pathocentrism, where established scientific norms intersect with ethical deliberations. Anthropocentrism, representative of conventional scientific approaches, may prioritize scientific goals potentially to the detriment of animal welfare. Conversely, pathocentrism places significant importance on the ethical treatment and well-being of AMs. This divergence of approach prompts the imperative development of a robust culture of care framework within research institutions, advocating for animal welfare, ethical responsibility, and adherence to regulatory standards. In this review, we refer to a European view of animal care, discussing internationally valid concepts that find rebuttal in the current European legislation. This review meticulously analyzes the many facets of the culture of care, particularly for neuroscience studies involving AMs, illustrating the principles, practices, and collaborations critical to overcoming ethical expectations. This commitment increases credibility and builds trust in the public and research spheres, underscoring the critical importance of a culture of care in the ethics of neuroscience research.
Spatial Conservation Prioritization of Persian Squirrel Based on Habitat Suitability and Climate-Induced Forest Mortality Risk
Authors: Ghadirian Baharanchi Omid , Hemami Mahmoud-Reza , Yousefpour Rasoul
Published: February 2024

The Zagros forests in Iran are currently experiencing an exacerbation of climate-induced mortality, placing the Persian squirrel, a keystone species reliant on these ecosystems, in jeopardy. Addressing this imminent threat, our research employed a spatial prioritization methodology, integrating assessments of habitat suitability and mortality risk. Utilizing a weighted ensemble approach, incorporating the strengths of diverse models and expert rules, we discerned that approximately 62% of surveyed forests are at risk, with 7% classified as high risk and 17% as very high risk. Notably, 83% of the forests exhibited varying degrees of habitat suitability, with 11% and 12% demonstrating high and very high suitability, respectively. Employing a conservation prioritization framework, we systematically categorized habitats into priority classes, with 8%, 17%, 29%, and 46% assigned to very high, high, moderate, and low conservation priority classes, respectively. Significantly, areas classified as very high priority demand immediate restoration efforts due to ongoing mortality, while other priority classes underscore the importance of protection and prevention in unaffected habitats. Acknowledging the irreversible nature of current climatic conditions in the Zagros forests, our strategic emphasis aligns with conservation triage principles, prioritizing the preservation of intact habitats yet to succumb to irreversible mortality.
Adaptation of the Coniferous Forests to Natural Fire Disturbances in the Altai Mountains, Xinjiang, China
Authors: Hong Ruicheng , Liang Ying , Wang Jin , Ma Cheng , Zhu Xilong , Xu Shiying , Yang Xu , Yeerna Asiwuhan , Wang Wendong , Wang Leiguang , Shu Lifu , Wang Mingyu , Wang Qiuhua
Published: February 2024

The Altai Mountains, located in the northwesternmost part of China, have a harsh climate and little human activity, making it an excellent location to study forest ecology undisturbed by human interference. The forest is frequently struck by lightning and experiences long-term natural fire disturbances, leading to the evolution of unique fire adaptation traits in the major conifer species. To explore the role of natural fire disturbances in the Altai Mountain forest ecosystem, we conducted a study on the fire adaptation traits of Larix sibirica, Pinus sibirica, Picea obovata, and Abies sibirica, and reconstructed the fire history of the forest area over the past 100 years. We investigated three representative forest areas with varying fire disturbance conditions and habitats in the Altai Mountains. Data on fire disturbance conditions, relative air humidity, and species composition were collected in these areas. Basal diameter to diameter at breast height, relative bark thickness, and under-crown heights were measured and counted for each of the four species, and litter, bark, and wood layers were sampled and analyzed for physicochemical properties (ash, fat, and higher heating value) for each of the four species in the plots examined. We conducted a count of the four conifer species in the forest for each fire adaptation index and analyzed the differences in fire adaptation traits among the species. Larix sibirica showed fire-tolerant traits, Pinus sibirica displayed fire-embracer traits, and Picea obovata and Abies sibirica exhibited fire-avoider traits. Through the analysis of stand composition and exposure to fire disturbance in the different forest areas, we were able to correlate the fire-adaptive strategies of the four conifers with stand characteristics under varying fire disturbances and habitats. The interaction between forests and fires, and their adaptation to each other, ultimately create the current ecosystems in the Altai Mountains.
Maximum Entropy Model Prediction of the Distributions of Two Sympatric Bean Weevil Species, Megabruchidius dorsalis (Fahraeus, 1839) and Bruchidius coreanus (Chûjô, 1937), under Various Climate Scenarios in Guizhou Province, China
Authors: Ma Guanying , Peng Qiyan , Pan Xiukui , Xie Minghui , Liao Jun , Wu Chengxu , Yang Maofa
Published: February 2024

Megabruchidius dorsalis and Bruchidius coreanus are sympatric bean weevil species that bore into Gleditsia sinensis seeds, seriously affecting the commercial development of this plant. Here, we aimed to understand potential changes in the distribution of these two sympatric pests under current and future climate conditions to provide a reference for the prediction of their occurrence and facilitate their prevention and control. Based on empirical field data, we predicted the potential distribution of M. dorsalis and B. coreanus in suitable habitat areas using the MaxEnt model and explored the relationships among different spatiotemporal distributions using change analysis. Our findings showed that compared with the current situation, the suitable areas for M. dorsalis and B. coreanus were predicted to increase by 4.8141% and 3.1009%, respectively, in the future. Isothermality (BIO3), min temperature of coldest month (BIO6), and variance in precipitation (BIO15) in the coldest month were determined to be the main factors restricting the current distribution of M. dorsalis and B. coreanus. Areas currently suitable for the two species are mainly in the central region of Guizhou and are predicted to move eastward in the future. Significant area under the receiver operating characteristics curve values for M. dorsalis (0.878) and B. coreanus (0.833) indicated that MaxEnt could be used to predict the potential habitats of these weevils, providing valuable information to inform their control in Guizhou Province.
Influence of Date Palm-Based Biochar and Compost on Water Retention Properties of Soils with Different Sand Contents
Authors: Le Guyader Elie , Morvan Xavier , Miconnet Vincent , Marin Béatrice , Moussa Mohamed , Intrigliolo Diego S. , Delgado-Iniesta María José , Girods Pierre , Fontana Sebastien , Sbih Mahtali , Boumaraf Belkacem , Tirichine Aissa , Kavvadias Victor , Gommeaux Maxime
Published: February 2024

Generally, soils of arid and semi-arid regions have low water retention properties due to high sand and low organic carbon contents. This study aimed at quantifying the effect of date palm-based organic amendments (OAs) on the water retention properties of two soils (sandy loam and silty loam), as well as the influence of sand supplementation (0.5–2 mm) on the magnitude of the effect of OAs. Different grain size distributions were obtained by adding sand to natural soils. For this purpose, sand was added to the two soils (1/3 and 2/3) and different soil-OA combinations were tested at a dose of 3% by mass: compost alone, biochar alone and a mixture of biochar and compost (50:50 in mass), in addition to unamended control soils. Soil water contents were measured at nine matric potentials ranging from the saturation to the permanent wilting point. Biochar was more efficient than compost at improving soil water retention. The effect of organic amendments on water retention increased with sand content. In most cases, soil water content values were significantly higher for biochar-amended soils than for unamended or compost-amended soils. The weakness of the effect of compost addition (if alone) was probably due to its properties and notably its high mineral content and electrical conductivity. Soil sand supplementation led to higher differences between the OA-amended soils and unamended soils. Changes in available water capacity reached +26% and +80% in a sandy loamy soil enriched with 2/3 sand and amended with compost and with biochar, respectively, compared to the unamended soil. These results show that sand content (and more generally, soil texture) influences the effect of OA application. Thus, the application of biochar from date palm residues in soil seems to be an effective solution to improve the water retention properties of coarse textured soils and contribute to optimizing the use of water resources in irrigated areas.
Utilization of Novel Basalt Fiber Pellets from Micro- to Macro-Scale, and from Basic to Applied Fields: A Review on Recent Contributions
Authors: Ahmed Tasnia , Bediwy Ahmed , Azzam Ahmed , Elhadary Riham , El-Salakawy Ehab , Bassuoni Mohamed T.
Published: February 2024

Fiber-reinforced cementitious composites (FRCC) are one of the leading engineering materials in the 21st century, as they offer proficiency in enhancing strength, ductility, and durability in structural engineering applications. Because the recently developed basalt fiber pellets (BFP) offer combined strands of fibers encased in a polymer matrix, they are being prevalently studied to explore new possibilities when used in brittle materials such as mortar and concrete. Hence, this paper synthesizes the intensive research efforts and contributions to this novel class of fibers conducted by the authors. Specifically, it reviews the fresh, mechanical, and durability properties of FRCC incorporating single BFP or hybrid with polyvinyl alcohol fibers and modified with slag/fly ash and nano-materials and its suitability for different field applications. In addition, the nano- and meso-scale modeling of such matrices are described. BFP significantly contributes to improving post-cracking flexural behavior by toughening the cementitious matrix and minimizing strength losses when exposed to harsh environments. All results show promising progress in the development of high-performance FRCC comprising BFP, with potential success for structural and pavement applications.
The Synergistic Effect of Compound Sugar with Different Glycemic Indices Combined with Creatine on Exercise-Related Fatigue in Mice
Authors: Liao Hui , Zhu Song , Li Yue , Huang Dejian
Published: February 2024

In this study, a compound sugar (CS) with different glycemic index sugars was formulated via hydrolysis characteristics and postprandial glycemic response, and the impact of CS and creatine emulsion on exercise-related fatigue in mice was investigated. Thirty-five C57BL/6 mice were randomly divided into five groups to supply different emulsions for 4 weeks: initial emulsion (Con), glucose emulsion (62 mg/10 g MW glucose; Glu), CS emulsion (62 mg/10 g MW compound sugar; CS), creatine emulsion (6 mg/10 g MW creatine; Cr), and CS and creatine emulsion (62 mg/10 g MW compound sugar, 6 mg/10 g MW creatine, CS-Cr). Then, the exhaustion time of weight-bearing swimming and forelimb grip strength were measured to evaluate the exercise capacity of mice, and some fatigue-related biochemical indexes of blood were determined. The results demonstrated that the ingestion of CS significantly reduced the peak of postprandial blood glucose levels and prolonged the energy supply of mice compared to ingesting an equal amount of glucose. Mouse exhaustion time was 1.22-fold longer in the CS group than in the glucose group. Additionally, the supplementation of CS increased the liver glycogen content and total antioxidant capacity of mice. Moreover, the combined supplementation of CS and creatine increased relative forelimb grip strength and decreased blood creatine kinase activity. The findings suggested that the intake of CS could enhance exercise capacity, and the combined supplementation of CS and creatine has a synergistic effect in improving performance.
Identification and Classification of Coix seed Storage Years Based on Hyperspectral Imaging Technology Combined with Deep Learning
Authors: Bai Ruibin , Zhou Junhui , Wang Siman , Zhang Yue , Nan Tiegui , Yang Bin , Zhang Chu , Yang Jian
Published: February 2024

Developing a fast and non-destructive methodology to identify the storage years of Coix seed is important in safeguarding consumer well-being. This study employed the utilization of hyperspectral imaging (HSI) in conjunction with conventional machine learning techniques such as support vector machines (SVM), k-nearest neighbors (KNN), random forest (RF), extreme gradient boosting (XGBoost), as well as the deep learning method of residual neural network (ResNet), to establish identification models for Coix seed samples from different storage years. Under the fusion-based modeling approach, the model’s classification accuracy surpasses that of visible to near infrared (VNIR) and short-wave infrared (SWIR) spectral modeling individually. The classification accuracy of the ResNet model and SVM exceeds that of other conventional machine learning models (KNN, RF, and XGBoost). Redundant variables were further diminished through competitive adaptive reweighted sampling feature wavelength screening, which had less impact on the model’s accuracy. Upon validating the model’s performance using an external validation set, the ResNet model yielded more satisfactory outcomes, exhibiting recognition accuracy exceeding 85%. In conclusion, the comprehensive results demonstrate that the integration of deep learning with HSI techniques effectively distinguishes Coix seed samples from different storage years.
Real-Time Monitoring of Dough Quality in a Dough Mixer Based on Current Change
Authors: Wang Wei , Zhou Xiaoling , Li Wenlong , Liang Jing , Huang Xiaowei , Li Zhihua , Zhang Xinai , Zou Xiaobo , Xu Bin , Shi Jiyong
Published: February 2024

Accurate assessment of dough kneading is pivotal in pasta processing, where both under-kneading and over-kneading can detrimentally impact dough quality. This study proposes an innovative approach utilizing a cost-effective current sensor to ascertain the optimal kneading time for dough. Throughout the kneading process, the dough’s tensile resistance gradually increases, reflecting the evolution of properties such as the gluten network. This leads to a discernible ascending phase in dough quality, evident through an increase in the load current of the mixing machine, succeeded by a subsequent decline beyond a certain threshold. The identification of this peak point enables the achievement of optimal dough consistency, thereby enhancing the overall quality of both the dough and subsequent pasta products. After the final product quality assessment, this novel method promises to be a valuable tool in optimizing pasta processing and ensuring consistent product quality.
Unlocking the Potential of Chemically Modified Carbon Gels in Gallic Acid Adsorption
Authors: Carvalho Regina C. , Durán-Valle Carlos J. , Adame-Pereira Marta
Published: February 2024

This study deals with the preparation of adsorbents from a commercial xerogel by chemically modifying its surface with concentrated mineral acids and alkali metal chlorides, their physicochemical characterization, and their use as adsorbents for gallic acid in aqueous solution. Although there are publications on the use of carbon xerogels as adsorbents, we propose and study simple modifications that can change their chemical properties and, therefore, their performance as adsorbents. The adsorbate of choice is gallic acid and, to our knowledge, there is no history of its adsorption with carbon xerogels. The prepared adsorbents have a high specific surface area (347–563 m2 g−1), better pore development for samples treated with alkali metal chlorides than with mineral acids, and are more acidic than the initial xerogel (p.z.c range 2.49–6.87 vs. 7.20). The adsorption equilibrium is reached in <16 h with a kinetic constant between 0.018 and 0.035 h−1 for the pseudo-second-order model. The adsorption capacity, according to the Langmuir model, reaches 62.89 to 83.33 mg g−1. The adsorption properties of the commercial xerogel improved over a wide range of pH values and temperatures. The experimental results indicate that the adsorption process is thermodynamically favored.
Extrusion-Based 3D Printing of Photocrosslinkable Chitosan Inks
Authors: García-García Ane , Pérez-Álvarez Leyre , Ruiz-Rubio Leire , Larrea-Sebal Asier , Martin Cesar , Vilas-Vilela José Luis
Published: February 2024

Photocuring of chitosan has shown great promise in the extrusion-based 3D printing of scaffolds for advanced biomedical and tissue engineering applications. However, the poor mechanical stability of methacrylated chitosan photocuring ink restricts its applicability. The inclusion of co-networks by means of simultaneous polycomplex formation is an effective method by which to solve this drawback, but the formed hydrogel inks are not printable. This work aims to develop new photocurable chitosan inks based on the simultaneous photocrosslinking of methacrylated chitosan (CHIMe) with N,N′-methylenebisacrylamide, polyethylene glycol diacrylate, and acrylic acid to be applied in extrusion 3D printing. Interestingly, the polycomplex co-network corresponding to the acrylic-acid-based ink could be successfully printed by the here-presented simultaneous photocuring strategy. Further, the conversion of photocrosslinking was studied via photo-DSC analyses that revealed a clear dependence on the chemical structure of the employed crosslinking agents (from 40 to ~100%). In addition, the mechanical and rheological properties of the photocured hydrogels were comparatively studied, as well as the printing quality of the extruded scaffolds. The newly developed chitosan photocurable inks demonstrated extrusion printability (squareness ~0.90; uniformity factor ~0.95) and tunable mechanical properties (Young modulus 14–1068 Pa) by means of different crosslinking approaches according to the chemical architecture of the reactive molecules employed. This work shows the great potential of photocrosslinkable chitosan inks.
Cross-Species Comparison of the Pan-RAF Inhibitor LY3009120’s Anti-Tumor Effects in Equine, Canine, and Human Malignant Melanoma Cell Lines
Authors: Gao Yu , Packeiser Eva-Maria , Wendt Sophia , Sekora Anett , Cavalleri Jessika-Maximiliane V. , Pratscher Barbara , Alammar Moosheer , Hühns Maja , Brenig Bertram , Junghanss Christian , Nolte Ingo , Murua Escobar Hugo
Published: February 2024

Malignant melanomas (MMs) are the abnormal proliferation of melanocytes and are one of the lethal skin cancers in humans, equines, and canines. Accordingly, MMs in companion animals can serve as naturally occurring animal models, completing conventional cancer models. The common constitutive activation of the MAPK and PI3K pathways in MMs has been described in all three species. Targeting the related pathways is considered a potential option in comparative oncologic approaches. Herein, we present a cross-species comparative analysis exposing a set of ten melanoma cell lines (one human, three equine, and six canine) derived from primary tumors or metastasis to a pan-RAF and RAF dimer inhibitor (LY3009120). Cellular response (proliferation, biomass, metabolism, early and late apoptosis/necrosis, and morphology) and the presence of pathogenic single-nucleotide variants (SNVs) within the mutational hotspot genes BRAF exon 11 and 15, NRAS exon 2 and 3, KRAS exon 2, and KIT exon 11 were analyzed. This study showed that equine malignant melanoma (EMM) cells (MelDuWi) harbor the KRAS p.Q61H mutation, while canine malignant melanoma (CMM) cells (cRGO1 and cRGO1.2) carry NRAS p.G13R. Except for EMM metastasis cells eRGO6 (wild type of the above-mentioned hotspot genes), all melanoma cell lines exhibited a decrease in dose dependence after 48 and 72 h of exposure to LY3009120, independent of the mutation hotspot landscape. Furthermore, LY3009120 caused significant early apoptosis and late apoptosis/necrosis in all melanoma cell lines except for eRGO6. The anti-tumor effects of LY3009120 were observed in nine melanoma cell lines, indicating the potential feasibility of experimental trials with LY3009120. The present study reveals that the irradiation-resistant canine metastasis cells (cRGO1.2) harboring the NRAS p.G13R mutation are significantly LY3009120-sensitive, while the equine metastases-derived eRGO6 cells show significant resistance to LY3009120, which make them both valuable tools for studying resistance mechanisms in comparative oncology.
Estrogenic Prenylated Flavonoids in Sophora flavescens
Authors: Nishi Kentaro , Imamura Ikumi , Hoashi Kenichiro , Kiyama Ryoiti , Mitsuiki Shinji
Published: February 2024

Sophora flavescens is a medicinal herb distributed widely in Japan and it has been used to treat various diseases and symptoms. To explore its pharmacological use, we examined the estrogenic activity of four prenylated flavonoids, namely kurarinone, kushenols A and I, and sophoraflavanone G, which are characterized by the lavandulyl group at position 8 of ring A, but have variations in the hydroxyl group at positions 3 (ring C), 5 (ring A) and 4’ (ring B). These prenylated flavonoids were examined via cell proliferation assays using sulforhodamine B, Western blotting, and RT-PCR, corresponding to cell, protein, and transcription assays, respectively, based on estrogen action mechanisms. All the assays employed here found weak but clear estrogenic activities for the prenylated flavonoids examined. Furthermore, the activities were inhibited by an estrogen receptor antagonist, suggesting that the activities were likely being mediated by the estrogen receptors. However, there were differences in the activity, attributable to the hydroxyl group at position 4’, which is absent in kushenol A. While the estrogenic activity of kurarinone and sophoraflavanone G has been reported before, to the best of our knowledge, there are no such reports on kushenols A and I. Therefore, this study represents the first report of their estrogenic activity.
Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs
Authors: Song Gang , Zhang Yuebo , Gao Hu , Fu Yawei , Chen Yue , Yin Yulong , Xu Kang
Published: February 2024

To investigate the differential immunology in Ningxiang and Berkshire pigs and their F1 offspring (F1 offspring), physiological and biochemical indicators in the plasma and spleen were analyzed. Then, transcriptomic analysis of the spleen identified 1348, 408, and 207 differentially expressed genes (DEGs) in comparisons of Ningxiang vs. Berkshire, Berkshire vs. F1 offspring, and Ningxiang vs. F1 offspring, respectively. In Ningxiang vs. Berkshire pigs, the gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEGs included CD163, MARCO, CXCL14, CCL19, and PPBP, which are associated with immunity. GO and KEGG analyses were also conducted comparing F1 offspring and their parents. The DEGs, including BPIFB1, HAVCR2, CD163, DDX3X, CCR5, and ITGB3, were enriched in immune-related pathways. Protein–protein interaction (PPI) analysis indicated that the EGFR and ITGA2 genes were key hub genes. In conclusion, this study identifies significant immune DEGs in different pig breeds, providing data to support the exploration of breeding strategies for disease resistance in local and crossbred pig populations.
A Pilot Study of Multiplex Ligation-Dependent Probe Amplification Evaluation of Copy Number Variations in Romanian Children with Congenital Heart Defects
Authors: Bolunduț Alexandru Cristian , Nazarie Florina , Lazea Cecilia , Șufană Crina , Miclea Diana , Lazăr Călin , Mihu Carmen Mihaela
Published: February 2024

Congenital heart defects (CHDs) have had an increasing prevalence over the last decades, being one of the most common congenital defects. Their etiopathogenesis is multifactorial in origin. About 10–15% of all CHD can be attributed to copy number variations (CNVs), a type of submicroscopic structural genetic alterations. The aim of this study was to evaluate the involvement of CNVs in the development of congenital heart defects. We performed a cohort study investigating the presence of CNVs in the 22q11.2 region and GATA4, TBX5, NKX2-5, BMP4, and CRELD1 genes in patients with syndromic and isolated CHDs. A total of 56 patients were included in the study, half of them (28 subjects) being classified as syndromic. The most common heart defect in our study population was ventricular septal defect (VSD) at 39.28%. There were no statistically significant differences between the two groups in terms of CHD-type distribution, demographical, and clinical features, with the exceptions of birth length, weight, and length at the time of blood sampling, that were significantly lower in the syndromic group. Through multiplex ligation-dependent probe amplification (MLPA) analysis, we found two heterozygous deletions in the 22q11.2 region, both in patients from the syndromic group. No CNVs involving GATA4, NKX2-5, TBX5, BMP4, and CRELD1 genes were identified in our study. We conclude that the MLPA assay may be used as a first genetic test in patients with syndromic CHD and that the 22q11.2 region may be included in the panels used for screening these patients.
Models for Considering the Thermo-Hydro-Mechanical-Chemo Effects on Soil–Water Characteristic Curves
Authors: Li Yao , Alves Roberto , Vanapalli Sai , Gitirana Gilson
Published: February 2024

The soil–water characteristic curve (SWCC) is widely used as a tool in geotechnical, geo-environmental, hydrology, and soil science fields for predicting and interpreting hydro-mechanical behaviors of unsaturated soils. Several previous studies focused on investigating the influence of initial water content, stress history, temperature, and salt content on the SWCC behavior. However, there is still limited understanding to be gained from the literature on how we can systematically incorporate the influence of complex thermo-hydro-mechanical-chemo (THMC) effects into interpreting and predicting the behavior of unsaturated soils. To address that knowledge gap, in this study, the coupled influence of temperature, initial stress state, initial density, soil structure, and chemical solution effects was modeled using established SWCC equations from the literature. The methodology for incorporating the coupled effects of these influential factors is presented herein. Furthermore, we evaluated the SWCC models proposed in this study, enabling us to provide a comprehensive discussion of their strengths and limitations, using the published SWCC data from the literature. The developments outlined in this paper contribute toward facilitating a rigorous approach for analyzing the THMC behaviors of unsaturated soils.
Applying Statistical Analysis and Economics Models to Unscramble the Depositional Signals from Chemical Proxies in Black Shales
Authors: Goldberg Karin , Goldberg Da Rosa Lucas
Published: February 2024

The complex controls on the accumulation of organic-rich rocks remain elusive, despite their economic importance as source rocks and unconventional reservoirs, partially due to the multitude of factors that may impact production and preservation of organic matter in sediments. The complexity of Earth systems is comparable to the intricacies of Economics, and application of statistical and econometrics methods and models to analyze geological data may assist interpretation of the processes controlling organic burial. Chemical indices calculated for mudrock datasets from modern sediments and the Woodford Formation were used as proxies for detrital input, primary productivity, redox conditions, and upwelling, and a series of statistical analyses were run to test whether these methods were useful to discriminate different depositional conditions and establish the controls on total organic carbon (TOC) in the sediments. Model results showed that chemical proxies reliably predict not only TOC but also indicate correlations between indices. Our results suggest that detrital input, primary productivity and bottom-water anoxia are relevant drivers of organic content in the sediments, but the first two appear to have a more significant role in organic burial, illustrating the usefulness of these methods to assess depositional parameters in organic-rich rocks.
Attempted Indigenous Erasure and Frontier Gothic in Arrival (2016)
Authors: Webster-Parmentier Bethany Jordan
Published: February 2024

In the process of adapting a written narrative for the silver screen, there is much that can be lost (or gained) in translation. Arrival, Denis Villeneuve’s adaption of Ted Chiang’s Story of Your Life, is no exception. Often analyzed as a work of science fiction, this article argues that understanding Arrival as a work of the frontier gothic renders the attempted erasure of Indigenous presence in the film visible. The frontier gothic elements of Arrival, most prominently the transformation of Chiang’s protagonist, Louise, into a frontier hero(ine), and the looming Montana setting, both evoke and attempt to erase the Indigenous presence in this “frontier”. As a frontier hero, Louise ultimately supersedes the aliens of Arrival, absorbing and appropriating their knowledge and language to save the world (and the superiority of the United States).
The Ancient Greek Sophists in Emanuele Tesauro’s Il cannocchiale aristotelico (1670): Thrasymachus and Gorgias
Authors: Katinis Teodoro
Published: February 2024

Emanuele Tesauro’s Il cannocchiale aristotelico (The Spyglass of Aristotle) is widely considered a masterpiece of the Baroque, mainly because of his theory of metaphor as a cognitive tool. But this work is much more than that. Tesauro presents his volume as the ultimate interpretation of Aristotle’s rhetorical art, which is clearly not the case. Indeed, his work is a polycentric discourse on a revolutionary theory of rhetoric that goes beyond any previous treatise written on the subject, including Aristotle’s Rhetoric. Despite his relevance in the history of rhetorical theories, Tesauro’s work is still waiting for a comprehensive study of its own as well as investigations of some of its specific aspects. Furthermore, the majority of the existing studies of Tesauro are in Italian (with only very few in English), which makes it difficult for this text to reach an international public. This essay explores what seems to be a specific aspect that has so far been almost completely neglected: the role played by the ancient sophists in the Cannocchiale aristotelico and in the history of rhetoric that Tesauro redesigns. Tesauro proclaims his fidelity to Aristotle but actually contradicts Aristotle’s anti-sophistic approach. During this analysis, we will discover even more about Tesauro’s pro-sophistic attitude: he grounds the climax of Latin rhetorical tradition in Greek sophistry. This positive assessment of the ancient sophists, especially Thrasymachus and Gorgias of Leontini, coexists with a critique of Socrates. Except for Sperone Speroni, no other early modern Italian author—or European author—has proposed this radical inversion of the canon established by Plato. This reversal makes Tesauro a relevant case study in the on-going exploration of the legacy of ancient sophists in Western literature.
A Posthuman Approach to BrexLit and Bordering Practices through an Analysis of John Lanchester’s The Wall
Authors: Alonso Alonso María
Published: February 2024

Kristen Sandrock (2020) connects John Lanchester’s 2019 Brexit novel The Wall with what she refers to as ‘British border epistemologies’; that is, a radical process of re-bordering due to global warming and its impact on human mobility. The literary phenomenon that is now referred to as ‘BrexLit’ bears witness to the way in which borders and the fear to the other seem to impinge on contemporary British fiction. BrexLit is framed by an increasing global interest in exploring interdisciplinary bordering practices. Primarily, BrexLit manifests through realist and/or speculative long fiction, although there are numerous short stories and poetry that deal with this seismic political event. This article proposes to focus on different samples of speculative long fiction born from Brexit before highlighting Lanchester’s The Wall. Posthuman studies offer a convenient theoretical framework with which to approach this specific text where the British border, refugees and the fear of the other are the drivers of the plot. Thus, this contribution will explore alien configurations of refugees in contemporary British speculative fiction and the way in which these texts question Brexit rhetoric in an eye-opening and thought-provoking way, assisting readers to understand the context and consequences of such a profound political event.
An Italian Validation of the 5-Item Attitudes to Mental Illness Questionnaire (AMIQ): A Useful Tool for Rapid Assessment of Stigma, Acceptance, and Tolerance
Authors: Stacchini Lorenzo , Fonzo Marco , Catalini Alessandro , Di Martino Giuseppe , Santangelo Omar Enzo , Menegon Tiziana , Cedrone Fabrizio , Gianfredi Vincenza
Published: February 2024

Stigma negatively impacts individuals, families, and communities, affecting relationships, education, and employment and leading to an additional burden on mental health. Assessing public attitudes towards people with mental health conditions is crucial, especially in terms of public health. Therefore, the Attitudes to Mental Illness Questionnaire (AMIQ) was validated and adapted to the Italian cultural context. Translation followed four phases, involving bilingual speakers, comparison, back-translation, and expert review. In the pre-test phase, the questionnaire was administered to 21 participants anonymously. The validation test involved 213 subjects. Statistical analyses included exploratory and confirmatory factor analysis, and McDonald’s Omega and Cronbach’s alpha to assess the internal consistency. The results indicate good internal consistency (Omega = 0.71; Alpha = 0.72), and confirmatory factor analysis (CFI = 0.971) validated the questionnaire’s construct. The study’s findings align with the original validation, underscoring the questionnaire’s robustness. Overall, understanding public attitudes is crucial for public health interventions combating stigma and fostering positive attitudes.
ISBAR: A Handover Nursing Strategy in Emergency Departments, Scoping Review
Authors: Chaica Veronica , Marques Rita , Pontífice-Sousa Patrícia
Published: February 2024

The present work aims to map the available scientific evidence on the benefits of using the ISBAR tool in the nursing care of acutely ill adult patients’ handover in an emergency department context. To this end, a scoping review was conducted, according to the guidelines proposed by the Joanna Briggs Institute (JBI), to answer the following research question: “What are the benefits of using the ISBAR tool in the nursing care of acutely ill adult patients’ handover in an emergency department context?” The bibliographic search was carried out during August and September 2023 in the following electronic databases: CINAHL Complete; MEDLINE Complete; Cochrane Central Register of Controlled Trials; Cochrane Database of Systematic Reviews; and Cochrane Methodology Register. Only works published between 2013 and 2023 were deemed fit for inclusion. All the included studies (9) show that ISBAR methodology, as a standardized tool for transferring nursing care in the emergency service, allows for a safe, clear, and concise transition of nursing care. The benefits relate to patient and professional safety, continuity, and quality of care, as well as patient and professional comfort, with health gains.
Adapting the Stress First Aid Model for Frontline Healthcare Workers during COVID-19
Authors: Bellehsen Mayer H. , Cook Haley M. , Shaam Pooja , Burns Daniella , D’Amico Peter , Goldberg Arielle , McManus Mary Beth , Sapra Manish , Thomas Lily , Wacha-Montes Annmarie , Zenzerovich George , Watson Patricia , Westphal Richard J. , Schwartz Rebecca M.
Published: February 2024

The coronavirus pandemic has generated and continues to create unprecedented demands on our healthcare systems. Healthcare workers (HCWs) face physical and psychological stresses caring for critically ill patients, including experiencing anxiety, depression, and posttraumatic stress symptoms. Nurses and nursing staff disproportionately experienced COVID-19-related psychological distress due to their vital role in infection mitigation and direct patient care. Therefore, there is a critical need to understand the short- and long-term impact of COVID-19 stress exposures on nursing staff wellbeing and to assess the impact of wellbeing programs aimed at supporting HCWs. To that end, the current study aims to evaluate an evidence-informed peer support stress reduction model, Stress First Aid (SFA), implemented across units within a psychiatric hospital in the New York City area during the pandemic. To examine the effectiveness of SFA, we measured stress, burnout, coping self-efficacy, resilience, and workplace support through self-report surveys completed by nurses and nursing staff over twelve months. The implementation of SFA across units has the potential to provide the workplace-level and individual-level skills necessary to reduce stress and promote resilience, which can be utilized and applied during waves of respiratory illness acuity or any other healthcare-related stressors among this population.
Behavioral Health Services Outcomes That Matter Most to Caregivers of Children, Youth, and Young Adults with Mental Health Needs
Authors: Graaf Genevieve , Kitchens Katherine , Sweeney Millie , Thomas Kathleen C.
Published: February 2024

This project documents the service outcomes that caregivers value most. A diverse group of caregivers, representing six regions of the United States, participated in two rounds of virtual one-hour focus groups. In round 1, participants identified what they hoped to gain from using behavioral health services for themselves, their families, and their child and discussed what made services a positive experience for them. They then reported their top-three most-hoped-for outcomes. In round 2, groups validated and refined summary findings from round 1. Caregivers prioritized service quality outcomes, primarily. They expressed a desire for an accessible, respectful, and supportive treatment environment, underpinned by well-trained and culturally responsive professionals. Caregivers also desire seamless cross-sector provider collaboration and care transitions, which integrate the insights and preferences of families and children themselves to craft a customized care plan. Priority outcomes not related to service quality included hoping to gain increased knowledge, resources, and tools and techniques to support the mental health needs of their children, to see their children improve their daily functioning and for their child develop more effective interpersonal communication skills. Caregivers also reported hoping to experience less stigma related to the mental health needs of their children and to achieve personal fulfillment for themselves and their children. Research, policies, and mental health services should prioritize and be designed to address the outcomes that matter to youth and families.