bluuebunny/crossref_metadata_2025 huggingface dataset:
Biliopancreatic Diversion (BPD), Long Common Limb Revisional Biliopancreatic Diversion (BPD + LCL–R), Roux-en-Y Gastric Bypass [RYGB] and Sleeve Gastrectomy (SG) mediate differential quantitative changes in body weight and qualitative modifications in body composition: a 5-year study
Authors: Ceriani Valerio , Pinna Ferdinando , Galantino Antonio , Zakaria Ahmed S. , Manfrini Roberto , Pontiroli Antonio E. , Folli Franco
Published: August 2021

Aims

Bariatric surgeries induce profound weight loss (decrease in body mass index, BMI), through a decrease in fat mass (FM) and to a much lesser degree of fat-free mass (FFM). Some reports indicate that the weight which is lost after gastric bypass (RYGB) and sleeve gastrectomy (SG) is at least partially regained 2 years after surgery. Here we compare changes in BMI and body composition induced by four bariatric procedures in a 5 years follow-up study.

Methods

We analyzed retrospectively modifications in BMI, FM and FFM obtained through Roux-en-Y gastric bypass (RYGB), sleeve gastrectomy (SG), biliopancreatic diversion (BPD) and a long common limb revisional biliopancreatic diversion (reduction of the gastric pouch and long common limb; BPD + LCL−R). Patients were evaluated at baseline and yearly for 5 years. Of the whole cohort of 565 patients, a subset of 180 patients had all yearly evaluations, while the remaining had incomplete evaluations. Setting University Hospital.

Results

In a total of 180 patients evaluated yearly for 5 years, decrease in BMI and FM up to 2 years was more rapid with RYGB and SG than BPD and BPD + LCL−R; with RYGB and SG both BMI and FM slightly increased in the years 3–5. At 5 years, the differences were not significant. When analysing the differences between 2 and 5 years, BPD + LCL−R showed a somewhat greater effect on BMI and FM than RYGB, BPD and SG. Superimposable results were obtained when the whole cohort of 565 patients with incomplete evaluation was considered.

Conclusions

All surgeries were highly effective in reducing BMI and fat mass at around 2 years; with RYGB and SG both BMI and FM slightly increased in the years 3–5, while BPD and BPD + LCL−R showed a slight further decreases in the same time interval.
Detector-based component model abstraction for microservice-based systems
Authors: Ntentos Evangelos , Zdun Uwe , Plakidas Konstantinos , Genfer Patric , Geiger Sebastian , Meixner Sebastian , Hasselbring Wilhelm
Published: August 2021

One of the chief problems in software architecture is avoiding architecture model drift and erosion in all kinds of complex software systems. Microservice-based systems introduce new challenges in this context, as they often use a large variety of technologies in their latest iteration, and are changed and released very frequently. Existing solutions that can be used to reconstruct architecture models fall short in addressing these new challenges, as they cannot easily cope with continuous evolution, their accuracy is too low, and highly polyglot settings are not supported well. In this work, we report on a research study aiming to design a highly accurate architecture model abstraction approach for comprehending component architecture models of highly polyglot systems that can cope with continuous evolution. After analyzing the results of related studies, we found two possible architecture model abstraction approaches that meet the requirements of our study: an opportunistic, and a reusable semi-automatic detector-based approach. We have conducted an empirical case study for validation and comparison of the two approaches. We conclude that both detector approaches are feasible. In our case study, the reusable approach breaks even in terms of time and effort needed for establishing reuse, if modest reuse of detectors is possible, and is producing slightly more high quality and evolution-stable solutions than the opportunistic approach.
How music may support perinatal mental health: an overview
Authors: Sanfilippo Katie Rose M. , Stewart Lauren , Glover Vivette
Published: August 2021

There is strong evidence that engaging with music can improve our health and well-being. Music-based interventions, approaches and practices, such as group music-making (singing or playing musical instruments), listening to music and music therapy, have all been shown to reduce symptoms of depression and anxiety. Although the existing literature needs expanding, mounting evidence suggests that music-based interventions, approaches and practices may help support maternal mental health prenatally and postnatally. The purpose of this descriptive overview is to provide a broad view of this area by bringing together examples of research across different practices and research disciplines. Selected evidence is examined, showing how music-based interventions, approaches and practices can reduce labour anxiety and pain, anxiety symptoms in pregnancy, postnatal depression symptoms and support maternal-infant bonding. The examined research includes single studies and reviews that use both qualitative and quantitative methods. Drawing on animal and human models, the effect of music on foetal behaviour and various possible biological, psychological and social mechanisms are discussed. The potential preventive effect of music-based interventions, approaches and practices and their possible use across different cultures are also considered. Overall, we highlight how music, employed in a variety of ways, may support perinatal mental health with the aim of stimulating more interest and research in this area.
Leveraging range joins for the computation of overlap joins
Authors: Dignös Anton , Böhlen Michael H. , Gamper Johann , Jensen Christian S. , Moser Peter
Published: August 2021

Joins are essential and potentially expensive operations in database management systems. When data is associated with time periods, joins commonly include predicates that require pairs of argument tuples to overlap in order to qualify for the result. Our goal is to enable built-in systems support for such joins. In particular, we present an approach where overlap joins are formulated as unions of range joins, which are more general purpose joins compared to overlap joins, i.e., are useful in their own right, and are supported well by B+-trees. The approach is sufficiently flexible that it also supports joins with additional equality predicates, as well as open, closed, and half-open time periods over discrete and continuous domains, thus offering both generality and simplicity, which is important in a system setting. We provide both a stand-alone solution that performs on par with the state-of-the-art and a DBMS embedded solution that is able to exploit standard indexing and clearly outperforms existing DBMS solutions that depend on specialized indexing techniques. We offer both analytical and empirical evaluations of the proposals. The empirical study includes comparisons with pertinent existing proposals and offers detailed insight into the performance characteristics of the proposals.
Influence of artificial aging: mechanical and physicochemical properties of dental composites under static and dynamic compression
Authors: Gornig D. C. , Maletz R. , Ottl P. , Warkentin M.
Published: August 2021

Objective

The aim of the study was to evaluate the influence of filler content, degradation media and time on the mechanical properties of different dental composites after in vitro aging.

Materials and Methods

Specimens (1 mm3) of three commercially available composites (GrandioSO®, Arabesk Top®, Arabesk Flow®) with respect to their filler content were stored in artificial aging media: artificial saliva, ethanol (60%), lactic acid (pH 5) and citric acid (pH 5). Parameters (Vickers microhardness, compressive strength, elastic modulus, water sorption and solubility) were determined in their initial state (control group, n = 3 for microhardness, n = 5 for the other parameters) and after 14, 30, 90 and 180 days (n = 3 for microhardness, n = 5 for the other parameters for each composite group, time point and media). Specimens were also characterized with dynamic-mechanical-thermal analysis (compression tests, F =  ± 7 N; f = 0.5 Hz, 1 Hz and 3.3 Hz; t = 0–170 °C).

Results

Incorporation of fillers with more than 80 w% leads to significantly better mechanical properties under static and dynamic compression tests and a better water sorption behavior, even after chemical degradation. The influence of degradation media and time is of subordinate importance for chemical degradation.

Conclusion

Although the investigated composites have a similar matrix, they showed different degradation behavior. Since dentine and enamel occur only in small layer thickness, a test specimen geometry with very small dimensions is recommended for direct comparison. Moreover, the use of compression tests to determine the mechanical parameters for the development of structure-compatible and functionally adapted composites makes sense as an additional standard.

Clinical relevance

Preferential use of highly filled composites for occlusal fillings is recommended.
The challenge of estimating wildlife populations at scale: the case of the European badger (Meles meles) in Ireland
Authors: Byrne Andrew W. , Parnell Andrew , O’Keeffe James , Madden Jamie M.
Published: August 2021

Estimating population size in space and time is essential for applied ecology and wildlife management purposes; however, making accurate and precise estimates at large scales is highly challenging. An example is the European badger (Meles meles), a widespread and abundant mammal in Ireland. Due to their role in the epidemiology of bovine tuberculosis, the species has been culled in agriculturally dominant landscapes with the intention of reducing spillback infection to local cattle populations. Despite several studies using different approaches having estimated badger populations at different time points and scales, there remains considerable uncertainty regarding the current population and its future trajectory. To explore this uncertainty, we use published data and expert opinion to estimate a snapshot of probable badger population size using a Monte Carlo approach, incorporating variation in three key components: social group numbers, group size, and culling efficacy. Using this approach, we estimate what the badger population in Ireland would be with/without culling, assuming a steady-state population at carrying capacity, and discuss the limitations of our current understanding. The mean estimate for the badger population size was 63,188 (5–95th percentile, 48,037–79,315). Population estimates were sensitive to the assumption of mean group size across landscape type. Assuming a cessation of culling (in favour of vaccination, for example) in agricultural areas, the mean estimated population size was 92,096 (5–95th percentile, 67,188–118,881). Despite significant research being conducted on badgers, estimates on population size at a national level in Ireland are only approximate, which is reflected in the large uncertainty in the estimates from this study and inconsistencies between recording of data parameters in previous studies. Focusing on carefully estimating group size, factors impacting its variation, in addition to understanding the dynamics of repopulation post-culling, could be a fruitful component to concentrate on to improve the precision of future estimates.
Age, microfacies and depositional environment of the Middle to Late Paleocene shallow-marine carbonates in the Sirt Basin of Libya (Upper Sabil Formation): “Are Intisar domal structures pinnacle reefs?”
Authors: Vršič A. , Gawlick H.-J. , Schlagintweit F. , Machaniec E. , Gharsalla M.
Published: August 2021

In the central-eastern Sirt Basin, enigmatic Intisar domal structures host significant hydrocarbon accumulations. These structures have been commonly interpreted as pinnacle reefs/bioherms occurring in the open-marine basinal environment. Generally, pinnacle reefs/bioherms are mainly characterized by in situ carbonates. The current study challenges the Intisar pinnacle reef/bioherm model by examining one of the domal structures in terms of biostratigraphy, microfacies and depositional environment. These structures were dated using larger benthic foraminifera, which yielded a Middle to Late Paleocene age (Selandian–Early Thanetian). Thirteen microfacies types representing different carbonate ramp environments ranging from outer ramp to inner ramp, were defined. Outer ramp deposits have been observed adjacent to the domal structure, represented mainly by wackestone with small benthic and planktonic foraminifera. The outer ramp deposits are most likely isochronous to the domal structures. The lower part of the domal structures is composed mainly of foraminiferal–algal–echinodermal packstones. The upper part is characterized by foraminiferal–algal–echinodermal packstones with intercalated microbialite–coral boundstones. The euphotic inner ramp deposits are preserved on the crest of the domal structure, consisting of grainstone and packstone rich inGlomalveolina. As a result of this study, the Intisar domal structures are seen as erosional relics of a carbonate ramp and no evidence for pinnacle reef/bioherm model was found.
A review of physical and engineering factors potentially affecting shear wave elastography
Authors: Nitta Naotaka , Yamakawa Makoto , Hachiya Hiroyuki , Shiina Tsuyoshi
Published: August 2021

It has been recognized that tissue stiffness provides useful diagnostic information, as with palpation as a screening for diseases such as cancer. In recent years, shear wave elastography (SWE), a technique for evaluating and imaging tissue elasticity quantitatively and objectively in diagnostic imaging, has been put into practical use, and the amount of clinical knowledge about SWE has increased. In addition, some guidelines and review papers regarding technology and clinical applications have been published, and the status as a diagnostic technology is in the process of being established. However, there are still unclear points about the interpretation of shear wave speed (SWS) and converted elastic modulus in SWE. To clarify these, it is important to investigate the factors that affect the SWS and elastic modulus. Therefore, physical and engineering factors that potentially affect the SWS and elastic modulus are discussed in this review paper, based on the principles of SWE and a literature review. The physical factors include the propagation properties of shear waves, mechanical properties (viscoelasticity, nonlinearity, and anisotropy), and size and shape of target tissues. The engineering factors include the region of interest depth and signal processing. The aim of this review paper is not to provide an answer to the interpretation of SWS. It is to provide information for readers to formulate and verify the hypothesis for the interpretation. Therefore, methods to verify the hypothesis for the interpretation are also reviewed. Finally, studies on the safety of SWE are discussed.
The plasticity of pancreatic cancer stem cells: implications in therapeutic resistance
Authors: Patil Kalyani , Khan Farheen B. , Akhtar Sabah , Ahmad Aamir , Uddin Shahab
Published: August 2021

The ever-growing perception of cancer stem cells (CSCs) as a plastic state rather than a hardwired defined entity has evolved our understanding of the functional and biological plasticity of these elusive components in malignancies. Pancreatic cancer (PC), based on its biological features and clinical evolution, is a prototypical example of a CSC-driven disease. Since the discovery of pancreatic CSCs (PCSCs) in 2007, evidence has unraveled their control over many facets of the natural history of PC, including primary tumor growth, metastatic progression, disease recurrence, and acquired drug resistance. Consequently, the current near-ubiquitous treatment regimens for PC using aggressive cytotoxic agents, aimed at ‘‘tumor debulking’’ rather than eradication of CSCs, have proven ineffective in providing clinically convincing improvements in patients with this dreadful disease. Herein, we review the key hallmarks as well as the intrinsic and extrinsic resistance mechanisms of CSCs that mediate treatment failure in PC and enlist the potential CSC-targeting ‘natural agents’ that are gaining popularity in recent years. A better understanding of the molecular and functional landscape of PCSC-intrinsic evasion of chemotherapeutic drugs offers a facile opportunity for treating PC, an intractable cancer with a grim prognosis and in dire need of effective therapeutic advances.
Suicide Ideation and Depression Quality of Life Ratings in a Reservation-Based Community of Native American Youths and Young Adults
Authors: Alfonso Y. N. , Bishai D. , Ivanich J. D. , O’Keefe V. M. , Usher J. , Aldridge L. R. , Haroz E. E. , Goklish N. , Barlow A. , Cwik M.
Published: August 2021

Suicide among adolescents is a significant public health concern in the U.S., especially within American Indian and Alaska Native (AIAN) communities. Lack of quality of life (QoL) estimates for both suicide ideation and depression specific to the AIAN population hinders the ability to compare interventions in cost-effectiveness analysis. We surveyed 200 AI youth and young adults from the Fort Apache Indian Reservation to estimate utility weights for experiencing suicide ideation and depression. Our results indicate that, on a scale of 0–100, with higher scores indicating better health, the general community rates both suicide ideation and depression at 15.8 and 25.1, respectively. These weights are statistically significantly different and lower than for other cultures. Culturally specific QoL values will allow the comparison and identification of the most effective and feasible interventions to reduce the suicide burden among tribal communities.
Negotiating (dis-)engagement in K-12 blended learning
Authors: Bergdahl Nina , Bond Melissa
Published: August 2021

It is well-recognised that engagement is critical for learning and school success. Engagement (and disengagement) are, however, also influenced by context. Thus, as digital technologies add complexity to the educational context, they influence classroom leadership, lesson designs and related practices, and thereby engagement. Despite being critical, engagement and disengagement are not well explored concerning these influences, with a lack of research undertaken within socially disadvantaged schools. In this qualitative study, 14 classroom observations were conducted, during five months, in twelve classes in an upper secondary school in Sweden, along with dialogues with teachers (n=12) and students (n=32). The data were analysed using thematic analysis and descriptive statistics. Identified themes include digital context, teacher leadership, engagement and disengagement. A network of relations between the (dis-)engagement compound and themes is presented. The results identified processes in which engagement shifted into disengagement and vice versa; in particular, that the intention of active learning does not automatically translate to active learning for all students, although teachers employed a higher work pace than did their students. Teacher self-efficacy and awareness of how to manage digital technologies in and outside the classroom was found to play a vital role in facilitating engagement. Understanding the (dis-)engagement compound in blended learning environments is key to inform active and visible learning for future research and supportive organisational structures.
Enhancement of root architecture and nitrate transporter gene expression improves plant growth and nitrogen uptake under long-term low-nitrogen stress in barley (Hordeum vulgare L.) seedlings
Authors: Gao Runhong , Guo Guimei , Xu Hongwei , Chen Zhiwei , Li Yingbo , Lu Ruiju , Liu Chenghong , Chen Jianmin
Published: August 2021

Over application of nitrogen (N) fertilizers to crops ultimately causes N pollution in the ecosphere. Studying the response of plant growth and N uptake to low-N stress may aid in elucidating the mechanism of low N tolerance in plants and developing crop cultivars with high nitrogen use efficiency (NUE). In this study, a high-NUE mutant line A9-29 and the wild-type barley cultivar Hua30 were subjected to hydroponic culture with high and low N supply, and the dry weight, N accumulation, root morphology, and expression levels of the potential genes involved in nitrate uptake and assimilation were measured at seedling stage. The results showed that under low-N conditions, A9-29 had a higher dry weight, N content, N influx rate and larger root uptake area than did Hua30. Under long-term low-N stress, compared with Hua30, A9-29 demonstrated higher expression of the HvNRT2/3 genes, especially HvNRT2.1, HvNRT2.5, and HvNRT3.3. Similarly, the expression levels of N assimilation genes including HvNIA1, HvNIR1, HvGS1_1, HvGS1_3, and HvGLU2 increased significantly in A9-29. Taken together, our results suggested that the larger root area and the upregulation of nitrate transporter and assimilation genes may contribute to stronger N uptake capacity for plant growth and N accumulation in responding to long-term low-N stress. These findings may aid in understanding the mechanism of low N tolerance and developing barley cultivars with high-NUE.
Authenticity work in higher education learning environments: a double-edged sword?
Authors: Hagvall Svensson Oskar , Adawi Tom , Johansson Anders
Published: August 2021

Educational authenticity occupies a strong position in higher education research and reform, building on the assumption that correspondence between higher education learning environments and professional settings is a driver of student engagement and transfer of knowledge beyond academia. In this paper, we draw attention to an overlooked aspect of authenticity, namely the rhetorical work teachers engage in to establish their learning environments as authentic and pedagogically appropriate. We use the term “authenticity work” to denote such rhetorical work. Drawing on ethnography and critical discourse analysis, we describe how two teachers engaged in authenticity work through renegotiating professional and educational discourse in their project-based engineering course. This ideological project was facilitated by three discursive strategies: (1) deficitization of students and academia, (2) naturalization of industry practices, and (3) polarization of the state of affairs in academia and in industry. Our findings suggest that authenticity work is a double-edged sword: While authenticity work may serve to bolster the legitimacy that is ascribed to learning environments, it may also close down opportunities for students to develop critical thinking about their profession and their education. Based on these findings, we discuss implications for teaching and propose a nascent research agenda for authenticity work in higher education learning environments.
Modern Colonialism and Cultural Continuity Through Material Culture: An Example from Guam and CHamoru Plaiting
Authors: Montón-Subías Sandra , Hernando Gonzalo Almudena
Published: August 2021

This article analyzes cultural persistence in Guam through plaiting, material culture, and maintenance activities, a set of daily practices that are essential to social continuity and well-being. The colonization of Guam began in 1668 with the Jesuit missions. Jesuit policies utilized maintenance activities to colonize Indigenous lifeways and subjectivities, but we believe those activities also functioned as reservoirs of traditional knowledge. Although plaiting has been situated in different historical contexts across the centuries, it no doubt expresses material continuities stretching from a precolonial past. The article also challenges today’s widespread belief that the search for change is a universal value. It argues that societies appreciate continuity over change in inverse proportion to technological control over nature, asymmetrical relationships of power, and specialized fragmentation of functional tasks. In the absence of such features, the best guarantee of survival lies in maintaining the balance achieved by traditional lifeways.
Distilling the Requirements of Gödel’s Incompleteness Theorems with a Proof Assistant
Authors: Popescu Andrei , Traytel Dmitriy
Published: August 2021

We present an abstract development of Gödel’s incompleteness theorems, performed with the help of the Isabelle/HOL proof assistant. We analyze sufficient conditions for the applicability of our theorems to a partially specified logic. In addition to the usual benefits of generality, our abstract perspective enables a comparison between alternative approaches from the literature. These include Rosser’s variation of the first theorem, Jeroslow’s variation of the second theorem, and the Świerczkowski–Paulson semantics-based approach. As part of the validation of our framework, we upgrade Paulson’s Isabelle proof to produce a mechanization of the second theorem that does not assume soundness in the standard model, and in fact does not rely on any notion of model or semantic interpretation.
Fabrication and characterization of an antibacterial chitosan/silk fibroin electrospun nanofiber loaded with a cationic peptide for wound-dressing application
Authors: Khosravimelal Sadjad , Chizari Milad , Farhadihosseinabadi Behrouz , Moosazadeh Moghaddam Mehrdad , Gholipourmalekabadi Mazaher
Published: August 2021

Wound infections are still problematic in many cases and demand new alternatives for current treatment strategies. In recent years, biomaterials-based wound dressings have received much attention due to their potentials and many studies have been performed based on them. Accordingly, in this study, we fabricated and optimized an antibacterial chitosan/silk fibroin (CS/SF) electrospun nanofiber bilayer containing different concentrations of a cationic antimicrobial peptide (AMP) for wound dressing applications. The fabricated CS/SF nanofiber was fully characterized and compared to the electrospun silk fibroin and electrospun chitosan alone in vitro. Then, the release rate of different concentrations of peptide (16, 32, and 64 µg/ml) from peptide-loaded CS/SF nanofiber was investigated. Finally, based on cytotoxic activity, the antibacterial activity of scaffolds containing 16 and 32 µg/ml of the peptide was evaluated against standard and multi-drug resistant strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa isolated from burn patients. The peptide-loaded CS/SF nanofiber displayed appropriate mechanical properties, high water uptake, suitable biodegradation rate, a controlled release without cytotoxicity on Hu02 human foreskin fibroblast cells at the 16 and 32 µg/ml concentrations of peptide. The optimized CS/SF containing 32 μg/ml peptide showed strong antibacterial activity against all experimental strains from standard to resistance. The results showed that the fabricated antimicrobial nanofiber has the potential to be applied as a wound dressing for infected wound healing, although further studies are needed in vivo.
Electrospun nano-fibrous bilayer scaffold prepared from polycaprolactone/gelatin and bioactive glass for bone tissue engineering
Authors: Elkhouly Hend , Mamdouh Wael , El-Korashy Dalia I.
Published: August 2021

This work is focused on integrating nanotechnology with bone tissue engineering (BTE) to fabricate a bilayer scaffold with enhanced biological, physical and mechanical properties, using polycaprolactone (PCL) and gelatin (Gt) as the base nanofibrous layer, followed by the deposition of a bioactive glass (BG) nanofibrous layer via the electrospinning technique. Electrospun scaffolds were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy. Surface area and porosity were evaluated using the nitrogen adsorption method and mercury intrusion porosimetry. Moreover, scaffold swelling rate, degradation rate and in vitro bioactivity were examined in simulated body fluid (SBF) for up to 14 days. Mechanical properties of the prepared scaffolds were evaluated. Cell cytotoxicity was assessed using MRC-5 cells. Analyses showed successful formation of bead-free uniform fibers and the incorporation of BG nanoparticles within fibers. The bilayer scaffold showed enhanced surface area and total pore volume in comparison to the composite single layer scaffold. Moreover, a hydroxyapatite-like layer with a Ca/P molar ratio of 1.4 was formed after 14 days of immersion in SBF. Furthermore, its swelling and degradation rates were significantly higher than those of pure PCL scaffold. The bilayer’s tensile strength was four times higher than that of PCL/Gt scaffold with greatly enhanced elongation. Cytotoxicity test revealed the bilayer’s biocompatibility. Overall analyses showed that the incorporation of BG within a bilayer scaffold enhances the scaffold’s properties in comparison to those of a composite single layer scaffold, and offers potential avenues for development in the field of BTE.
In vitro and in vivo biological performance of hydroxyapatite from fish waste
Authors: Prado João Paulo dos Santos , Yamamura Hirochi , Magri Angela Maria Paiva , Ruiz Pedro Luiz Muniz , Prado José Lucas dos Santos , Rennó Ana Claudia Muniz , Ribeiro Daniel Araki , Granito Renata Neves
Published: August 2021

The aim of this study was to evaluate biocompatibility of hydroxyapatite (HAP) from fish waste using in vitro and in vivo assays. Fish samples (whitemouth croaker - Micropogonias furnieri) from the biowaste was used as HAP source. Pre-osteoblastic MC3T3-E1 cells were used in vitro study. In addition, bone defects were artificially created in rat calvaria and filled with HAP in vivo. The results demonstrated that HAP reduced cytotoxicity in pre-osteoblast cells after 3 and 6 days following HAP exposure. DNA concentration was lower in the HAP group after 6 days. Quantitative RT-PCR did not show any significant differences (p > 0.05) between groups. In vivo study revealed that bone defects filled with HAP pointed out moderate chronic inflammatory cells with slight proliferation of blood vessels after 7 and 15 days. Chronic inflammatory infiltrate was absent after 30 days of HAP exposure. There was also a decrease in the amount of biomaterial, being followed by newly formed bone tissue. All experimental groups also demonstrated strong RUNX-2 immoexpression in the granulation tissue as well as in cells in close contact with biomaterial. The number of osteoblasts inside the defect area was lower in the HAP group when compared to control group after 7 days post-implantation. Similarly, the osteoblast surface as well as the percentage of bone surface was higher in control group when compared with HAP group after 7 days post-implantation. Taken together, HAP from fish waste is a promising possibility that should be explored more carefully by tissue-engineering or biotechnology.
Surface properties of a new lithium disilicate glass-ceramic after grinding
Authors: Miotto Larissa Natiele , Villas-Bôas Mariana de Oliveira Carlos , Zanotto Edgar Dutra , Ferreira Eduardo Bellini , Fais Laiza Maria Grassi , Pinelli Lígia Antunes Pereira
Published: August 2021

This study aimed to evaluate the effect of grinding on some surface properties of two lithium disilicate-based glass-ceramics, one experimental new product denominated LaMaV Press (UFSCar-Brazil) and another commercial known as IPS e-max Press (Ivoclar), in the context of simulated clinical adjustment. Discs (N = 24, 12 mm in diameter) were separated into four groups: LaMaV Press with no grinding (E), LaMaV Press after grinding (EG), IPS e-max Press with no grinding (C), and IPS e-max Press after grinding (CG). A 0.1-mm deep grinding was carried out on EG and CG samples (final thickness of 1.4 mm) using a diamond stone in a low-speed device. The E and C samples had the same thickness. The effect of grinding on the sample surfaces was evaluated by X-ray diffraction, mechanical and optical profilometry, scanning electron microscopy, goniometry, and Vickers hardness. The mean roughness (Ra) was evaluated by Kruskal–Wallis and Student–Newman–Keuls statistics. The surface energy (SE) by the sessile drop method and Vickers hardness (VH) were analyzed using two-way ANOVA. The Ra medians were E = 1.69 µm, EG = 1.57 µm, C = 1.45 µm, and CG = 1.13 µm with p = 0.0284. The SE and VH were similar for all materials and treatments. Grinding smoothed the surfaces and did not significantly alter the hardness and surface energy of both LaMaV Press and IPS e-max Press. These glass-ceramics presented similar surface properties, and clinical adjustments can be implemented without loss of performance of both materials.
Polyethylene glycol triggers the anti-cancer impact of curcumin nanoparticles in sw-1736 thyroid cancer cells
Authors: Hosseinzadeh Simzar , Nazari Hojjatollah , Esmaeili Elaheh , Hatamie Shadie
Published: August 2021

Curcumin has been recognized as an effective anticancer agent. However, due to its hydrophobic property, the cell absorption is not satisfied. Herein, the curcumin nanoparticles were prepared in the presence of polyethylene glycol 6000 (PEG6000) to reduce its elimination by immune system. For first time, not only the curcumin was encapsulated within the niosome nanoparticles modified by PEG, there are no reports related to the anticancer property of curcumin against thyroid cancers. The nanoparticles was developed and its anticancer was studied on sw-1736 cancer cell line. The nanoparticles were examined by scanning electron microscopy (SEM) and dynamic light scattering (DLS). Also, the release profile of curcumin, the IC50 concentration, the radical amount and the gene expression were evaluated. The optimized nanoparticles showed a diameter of 212 ± 31 nm by SEM and the encapsulation efficiency and loading capacity of 76% and 16.8% respectively. DLS confirmed the polydispersity index (PDI) of 0.596 and the release model was shown a sustained release with the delivery of 68% curcumin after 6 days. Also, the nanoparticles indicated the higher storage stability at 4 °C. After the cell treatment, the apoptotic bodies were appeared and IC50 was obtained as 0.159 mM. Moreover, the generated radicals by the treated cells was 86% after 72 h and the gene pattern indicated the bax/bcl2 ratio of 6.83 confirming the apoptosis effect of the nanoparticles. The results approved the nanoparticles could be suggested as an anticancer drug candidate for thyroid cancers.
Hidden invariant convexity for global and conic-intersection optimality guarantees in discrete-time optimal control
Authors: Baayen Jorn H. , Postek Krzysztof
Published: August 2021

Non-convex discrete-time optimal control problems in, e.g., water or power systems, typically involve a large number of variables related through nonlinear equality constraints. The ideal goal is to find a globally optimal solution, and numerical experience indicates that algorithms aiming for Karush–Kuhn–Tucker points often find solutions that are indistinguishable from global optima. In our paper, we provide a theoretical underpinning for this phenomenon, showing that on a broad class of problems the objective can be shown to be an invariant convex function (invex function) of the control decision variables when state variables are eliminated using implicit function theory. In this way, optimality guarantees can be obtained, the exact nature of which depends on the position of the solution within the feasible set. In a numerical example, we show how high-quality solutions are obtained with local search for a river control problem where invexity holds.
Evaluation of mesostructured silica materials with different structures and morphologies as carriers for quercetin and naringin encapsulation
Authors: Morante-Zarcero Sonia , Endrino Alba , Casado Natalia , Pérez-Quintanilla Damián , Sierra Isabel
Published: August 2021

Two mesostructured silicas with wormhole-like pore arrangement (HMS and MSU-2) were synthesized and evaluated for the first time as carriers for the encapsulation of two bioactive flavonoids (quercetin and naringin). For comparative purposes, a hexagonal mesostructured SBA-15 silica type frequently used as encapsulating support was also prepared and tested. All the materials were characterized before and after the loading with the analytes. Different silica/analyte ratios were evaluated to determine the loading and encapsulation kinetics of the different materials. Both flavonoids were successfully loaded inside the pores of the three silicas. The quercetin loading capacity of HMS was higher than SBA-15 and MSU-2 silicas, whereas for naringin SBA-15 and MSU-2 were slightly more effective. These differences could be attributed to the molecular size of the analytes and the textural properties of the different materials. Nevertheless, HMS was the silica that enabled to release the highest amount of both analytes. Thus, it could be considered a suitable carrier of these flavonoids and an alternative to other materials such as SBA-15. Moreover, the release process was performed under controlled conditions (pH 2.0 and 7.4) to simulate digestive conditions. Quercetin was delivered faster and more efficiently from the encapsulated at pH 2.0, whereas no differences were observed for naringin at both pHs. Finally, the antioxidant activity of the resulting encapsulates was determined. The results obtained suggested the potential use of wormhole-like mesostructured silicas as carriers to enhance the stability and bioavailability of flavonoids, so they can be used in future food and biomedical applications.
Verifying Negative Sentences
Authors: Wang Shenshen , Sun Chao , Tian Ye , Breheny Richard
Published: August 2021

In the long history of psycholinguistic research on verifying negative sentences, an often-reported finding is that participants take longer to correctly judge negative sentences true than false, while being faster to judge their positive counterparts true (e.g. Clark & Chase, Cogn Psychol 3(3):472−517, 1972; Carpenter & Just, Psychol Rev 82(1):45–73, 1975). While many linguists and psycholinguists have strongly advocated the idea that the costs and complexity of negation can be explained by appeal to context, context-based approaches have not been able to provide a satisfying account of this polarity*truth-value interaction. By contrast, the alternative theory of negation processing, which says that negation is processed by separately representing the positive, does provide a plausible account. Our proposals provide a means for reconciliation between the two views since we argue that negation is a strong cue to a positive context. Here we present our account of why and when negation is often apparently processed via the positive. We review many of the factors that are seen to be at play in sentence verification involving negation. We present evidence that participants’ adoption of the positive-first procedure in sentence-picture verification tasks is conditioned by context.
Translation and Validation of the Spanish Version of the Spiritual Care Competence Questionnaire (SCCQ)
Authors: Pastrana Tania , Frick Eckhard , Krikorian Alicia , Ascencio Leticia , Galeazzi Florencia , Büssing Arndt
Published: August 2021

We aimed to validate the Spanish version of the Spiritual Care Competence Questionnaire (SCCQ) in a sample of 791 health care professionals from Spanish speaking countries coming principally from Argentina, Colombia, Mexico and Spain. Exploratory factor analysis pointed to six factors with good internal consistency (Cronbach’s alpha ranging from 0.71 to 0.90), which are in line with the factors of the primary version of the SCCQ. Conversation competences and Perception of spiritual needs competences scored highest, and Documentation competences and Team spirit the lowest, Empowerment competences and Spiritual self-awareness competences in-between. The Spanish Version of the SCCQ can be used for assessment of spiritual care competencies, planning of educational activities and for comparisons as well as monitoring/follow-up after implementation of improvement strategies.
Dynamical Renormalization Group for Mode-Coupling Field Theories with Solenoidal Constraint
Authors: Cavagna Andrea , Di Carlo Luca , Giardina Irene , Grigera Tomas , Pisegna Giulia , Scandolo Mattia
Published: August 2021

The recent inflow of empirical data about the collective behaviour of strongly correlated biological systems has brought field theory and the renormalization group into the biophysical arena. Experiments on bird flocks and insect swarms show that social forces act on the particles’ velocity through the generator of its rotations, namely the spin, indicating that mode-coupling field theories are necessary to reproduce the correct dynamical behaviour. Unfortunately, a theory for three coupled fields—density, velocity and spin—has a prohibitive degree of intricacy. A simplifying path consists in getting rid of density fluctuations by studying incompressible systems. This requires imposing a solenoidal constraint on the primary field, an unsolved problem even for equilibrium mode-coupling theories. Here, we perform an equilibrium dynamic renormalization group analysis of a mode-coupling field theory subject to a solenoidal constraint; using the classification of Halperin and Hohenberg, we can dub this case as a solenoidal Model G. We demonstrate that the constraint produces a new vertex that mixes static and dynamical coupling constants, and that this vertex is essential to grant the closure of the renormalization group structure and the consistency of dynamics with statics. Interestingly, although the solenoidal constraint leads to a modification of the static universality class, we find that it does not change the dynamical universality class, a result that seems to represent an exception to the general rule that dynamical universality classes are narrower than static ones. Our results constitute a solid stepping stone in the admittedly large chasm towards developing an off-equilibrium mode-coupling theory of biological groups.
Pixel-wise parallel calculation for depth from focus with adaptive focus measure
Authors: Matsubara Yoichi , Shirai Keiichiro , Ito Yuya , Tanaka Kiyoshi
Published: August 2021

Depth-from-focus methods can estimate the depth from a set of images taken with different focus settings. We recently proposed a method that uses the relationship of the ratio between the luminance value of a target pixel and the mean value of the neighboring pixels. This relationship has a Poisson distribution. Despite its good performance, the method requires a large amount of memory and computation time because it needs to store focus measurement values for each depth and each window radius on a pixel-wise basis, and filtering to compute the mean value, which is performed twice, makes the relationship among neighboring pixels too strong to parallelize the pixel-wise processing. In this paper, we propose an approximate calculation method that can give almost the same results with a single time filtering operation and enables pixel-wise parallelization. This pixel-wise processing does not require the aforementioned focus measure values to be stored, which reduces the amount of memory. Additionally, utilizing the pixel-wise processing, we propose a method of determining the process window size that can improve noise tolerance and in depth estimation in texture-less regions. Through experiments, we show that our new method can better estimate depth values in a much shorter time.
Delay-induced bifurcations in collocated position control of an elastic arm
Authors: Szaksz Bence , Stepan Gabor
Published: August 2021

The interference of the elasticity of a single robotic arm and the unavoidable time delay of its position control is analysed from nonlinear vibrations viewpoint. The simplified mechanical model of two blocks and a connecting spring considers the first vibration mode of the arm, while the collocated proportional-derivative (PD) control uses the state of the first block only and actuates also there. It is assumed that the relevant nonlinearity is the saturation of the delayed control force. The linear stability analysis proves that stabilizable and non-stabilizable parameter regions follow each other periodically even for large spring stiffnesses and for tiny time delays. Hopf bifurcation calculation is carried out after an infinite-dimensional centre manifold reduction, and closed-form algebraic expressions are given for the amplitudes of the emerging oscillations. These results support the experimental tuning of the control gains since the parameters of the arising and often unexpected self-excited vibrations can serve as a guide for this practical procedure.
Alkaloid diversification in the genus Palicourea (Rubiaceae: Palicoureeae) viewed from a (retro-)biogenetic perspective
Authors: Berger Andreas , Valant-Vetschera Karin , Schinnerl Johann , Brecker Lothar
Published: August 2021

The species-rich genus Palicourea (Rubiaceae: Palicoureeae) is source of an intriguing diversity of alkaloids derived from tryptamine and its precursor tryptophan. So far simple tryptamine analogues, polypyrroloindoline, β-carboline, and, most importantly, monoterpene-indole, i.e., tryptamine-iridoid alkaloids of various structural types including javaniside, alstrostine and strictosidine derivatives have been identified. Here the diverse alkaloids that numerous studies have found in the genus are examined and organized according to their structures and biosynthetic groups. Using a parsimony-based approach that follows the concept of retro-biogenesis usually applied in synthetic chemistry, possible biosynthetic pathways are proposed and important steps and relationships between these alkaloids are highlighted. Understanding alkaloid diversification is of importance in studying the ecological significance and evolution of biosynthetic capabilities of the genus Palicourea, and should stimulate future investigations on the biochemical and genetic background.
Evaluation and GWAS of radicle gravitropic response in a core rice germplasm population
Authors: Lou Qiaojun , Li Qingsong , Feng Fangjun , Joynson Ryan , Yang Yunan , Luo Lijun , Chen Liang
Published: August 2021

Aims

Since gravitropism is one of the primary determinants of root development, facilitating root penetration into soil and subsequent absorption of water and nutrients, we studied this response in rice.

Methods

The gravitropism of 226 Chinese rice micro-core accessions and drought-resistant core accessions were assessed through the modified gravity-bending experiment and genome-wide association analysis (GWAS) was used to map the associated QTLs.

Results

The average value of gravitropic response speed of seminal roots was 41.05°/h, ranging from 16.77°/h to 62.83°/h. The gravity response speed of Indica (42.49°/h) was significantly (P < 0.002) higher than Japonica (39.71°/h) subspecies. The gravitational response speed of seminal roots was significantly positively correlated with the number of deep roots (r = 0.16), the growth speed of seminal roots (r = 0.21) and the drought resistance coefficient (r = 0.14).

Conclusions

In total, 3 QTLs (quantitative traits) associated with gravitropic response speed were identified on chromosome 4, 11 and 12. There are some known QTLs relating to roots traits and drought resistance located nearby the QTLs identified here, which confirms the close relationship between radicle gravitropism and the drought resistance. From within these intervals, 5 candidate genes were screened and verified by qPCR in a few rice varieties with extreme phenotypic values, demonstrating that gene LOC_Os12g29350 may regulate gravitropism negatively. This may be a promising candidate to be confirmed in further studies.