bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Dynamics Simulations and MM/PBSA Analysis of Annocatacin B in ND1 Subunit of Human Mitochondrial Respiratory Complex I
Authors: Febres-Molina Camilo , Aguilar-Pineda Jorge A. , Gamero-Begazo Pamela L. , Barazorda-Ccahuana Haruna L. , Valencia Diego E. , Vera-López Karin J. , Dávila-Del-Carpio Gonzalo , Gómez Badhin
Published: May 2021

ND1 subunit possesses the majority of the inhibitor binding domain of the human MRC-I. This is an attractive target for the search for new inhibitors that seek mitochondrial dysfunction. It is known, from in vitro experiments, some metabolites from Annona muricata called acetogenins have important biological activities such as anticancer, antiparasitic, and insecticide. Previous studies propose an inhibitory activity of bovine MRC-I by bis-THF acetogenins such as annocatacin B, however, there are few studies on its inhibitory effect on human MRC-I. In this work, we evaluate the molecular and energetic affinity of the annocatacin B molecule with the human ND1 subunit in order to elucidate its potential capacity to be a good inhibitor of this subunit. For this purpose, QM optimizations, MD simulations and MM/PBSA analysis were performed. As a control to compare our outcomes, the molecule rotenone, which is a known MRC-I inhibitor, was chosen. Our results show that annocatacin B has a greater affinity for the ND1 structure, its size and folding were probably the main characteristics that contributed to stabilize the molecular complex. Furthermore, the MM/PBSA calculations showed a 35% stronger BFE compared to the rotenone complex. Detailed analysis of the BFE shows that the aliphatic chains of annocatacin B play a key role in molecular coupling by distributing favorable interactions throughout the major part of the ND1 structure. These results are consistent with experimental studies that mention that acetogenins may be good inhibitors of MRC-I.
Evaluation of Thiol-dependent Enzymes on the Pharmacological Effects Induced by the Catalytically Active PLA2 from Bothrops jararacussu
Authors: Toyama Marcos Hikari , Costa Caroline R. C. , Belchor Mariana Novo , Junior Adeilso Bispo dos Santos , Moraes Laila Lucyane Ferreira de , Silva Airam Roggero dos Santos , Oliveira Marcos Antonio de
Published: May 2021

Background: Clinical cases reports with snake accidents show that venom bite induces increased oxidative stress including several markers of lipid peroxidation and other oxidative stress marker in plasma. Methods: The main findings of this work were performed with BthTx-II on paw edema of animals treated with the toxin and biochemical measurement of COX-2, PGE2, MDA and the effects of peroxiredoxin inhibitors on edema and myotoxicity were also evaluated. Results: The results show that edema and myotoxocity induced by PLA2 (BthTx-II) induces a strong mobilization of arachidonic acid and an increase in cellular oxidative stress as measured by increased malondialdehydo (MDA) concentration and protein carbonylation. Thus, these findings establish the strong link between oxidative stress, arachidonic acid mobilization and that these events may explain the presence of oxidative stress markers in snake-bitten patients. Experiments performed with animals previously treated with commercially purchased inhibitors showed enzymes such as thioredoxin (TXN), thioredoxin reductase (TXNRD) and other glutathione (GSH)-related antioxidant defenses could play an essential role controlling and defining the end of edema on the late phase of PLA2 BthTx-II-induced process. Conclusion. This study showed that thioate-dependent antioxidant enzymes play an important role in resolving the edema induced by BthTx-II.
Hidden from Our Sight, but Not from Our Impact: The Conservation Issues of Cave Bivalves
Authors: Bilandžija Helena , Puljas Sanja , Gerdol Marco
Published: May 2021

Groundwater habitats in the Dinaric Karst are home to the only known cave-adapted genus of bivalve mollusks, which currently survives as three distinct species with a highly fragmented distribution. Over the past few decades, Congeria populations suffered a steep decline across their range, as a result of human activities. Here, we identify the most pressing issues concerning the current conservation status of Congeria and identify key priorities for its scientific study. The building of dams and other hydrotechnical constructions have led to a significant decrease in the water inputs that used to supply underground systems where Congeria lives, contributing to a reduction of the cave habitats feasible for bivalve settlement. This is relatively well documented in Popovo polje where, as a result of hydrotechnical interventions on Trebisnjica river, water level drop destroyed well over 99 % of Congeria kusceri population in Zira cave and caused their complete disappearance in other caves. Similar factors are likely to have also affected the sister species Congeria jalzici in the Lika region. In addition, the salinization of Neretva river, lack of proper wastewater management, intense agriculture, tourist exploitation, and additional hydrotechnical plans add to the ongoing decline of the quality of Congeria habitats. These threats are expected to least to drastic reductions in population size within the next few decades, and this situation might be further aggravated by the lack of regulations and active measures for Congeria protection, as well by the limited understanding of its biology. Future scientific studies should be aimed at better elucidating microhabitat preference, defining the range of tolerance of environmental parameters, and assessing the size and genetic viability of existing Congeria populations. However, we highlight that these efforts would most certainly require the collaboration of local authorities and the allocation of appropriate financial resources in order to be effective.
Taking Stock of Some Recent and Notable Contribution to Research in Portfolio Analysis
Authors: Wirjanto Tony S. , Guo Danqiao , Weng Chengguo
Published: May 2021

In this paper we provide a highly selected review and synthesis on some of the recent and notable

contribution to research in portfolio analysis. A unique perspective on this development

in the literature is offered in this paper by judiciously identifying a few sample eigenvalues

adjustment patterns in a portfolio that leads to an improvement in the out-of-sample portfolio

Sharpe ratio when the population covariance matrix admits a high-dimensional factor model.

These patterns unveil a key insight into a portfolio performance improvement and shed an

important light on the effectiveness of a few recently introduced ”robust to estimation errors”

covariance matrix estimation approaches, which were not originally designed with the goal to

improve the out-of-sample portfolio performance.
Physiological and Biochemical Markers of the Gender-specific Sensitivity to Epileptogenic Factors, Delayed Consequences of Seizures and Their Response to Vitamins B1 and B6 in a Rat Model of Epilepsy
Authors: Aleshin Vasily A. , Graf Anastasia V. , Artiukhov Artem V. , Boyko Alexandra I. , Ksenofontov Alexander L. , Maslova Maria V. , Nogués Isabel , di Salvo Martino L. , Bunik Victoria I.
Published: May 2021

Disturbed metabolism of vitamins B1 or B6, which are essential for neurotransmitters homeostasis, may cause epilepsy. Our study aims at revealing therapeutic potential of vitamins B1 and B6 in epilepsy by estimating effects of their combined administration on a seizure and its consequences in rats subjected to pentylenetetrazole (PTZ). The PTZ dose dependence of a seizure and its parameters according to Racine’s scale along with delayed physiological and biochemical consequences next day after the seizure are assessed regarding sexual dimorphism in epilepsy. PTZ sensitivity is stronger in the female than male rats. Next day after a seizure, gender differences in behavior and brain biochemistry arise. The induced gender differences in anxiety, exploratory and locomotor activity correspond to disappearance of gender differences in the brain GABA, aspartate, alanine and serine, with appearance of those in glutamate, glutamine and tyrosine. PTZ decreases the brain malate dehydrogenase activity, glutamine and urea in the males, and phenylalanine in the females. Administration of vitamins B1 and B6 24 h before PTZ delays a seizure in female rats only. This desensitization is not observed at short intervals (0.5-2 h) between the vitamins and PTZ administration. With the increasing interval, the pyridoxal kinase (PLK) activity in the female brain decreases, suggesting that the PLK downregulation by vitamins contributes to the desensitization. Delayed effects of vitamins and/or PTZ are mostly gender-specific and interacting. Our findings on the gender differences in sensitivity to epileptogenic factors, action of vitamins B1/B6 and associated biochemical events have medical implications.
A Primer on Harnessing Non-enzymatic Posttranslational Modifications for Drug Design
Authors: Long Marcus , Ly Phillippe , Aye Yimon
Published: May 2021

Of the manifold concepts in drug discovery and design, covalent drugs have re-emerged as one of the most promising over the past 20-or so years. All such drugs harness the ability of a covalent bond to drive an interaction between a target biomolecule, typically a protein, and a small molecule. Formation of a covalent bond necessarily prolongs target engagement, opening avenues to targeting shallower binding sites, protein complexes, and other difficult to drug manifolds, amongst other virtues. This opinion piece discusses frameworks around which to develop covalent drugs. Our argument, based on results from our research program on natural electrophile signaling, is that targeting specific residues innately involved in native signaling programs are ideally poised to be targeted by covalent drugs. We outline ways to identify electrophile-sensing residues, and discuss how studying ramifications of innate signaling by endogenous molecules can provide a means to predict drug mechanism and function and assess on- versus off-target behaviors.
Effect of Productive Capacities on Economic Resilience in Developing Countries
Authors: Gnangnon Sena Kimm
Published: May 2021

The COVID-19 outbreak and its economic, social and financial fallouts have generated a renewed interest in finding adequate policies and means to strengthen economic resilience to future shocks, particularly in developing countries. The latter are usually disproportionately affected by adverse shocks (compared to developed countries) and lack the adequate resources to weather these shocks. Strengthening economic resilience is now at the heart of the policy discussion both at the national and international levels. The present paper aims to contribute to this debate by investigating the effect of productive capacities on economic resilience in a panel dataset of 118 developing countries over the period 2000-2018. It constructs a regression-based economic resilience indicator, and makes use of the indicator of productive capacities recently developed by the UNCTAD. Results are quite interesting, including from a policy perspective. The development of productive capacities is associated with greater economic resilience. This is particularly the case for countries with greater trade openness, greater capital account openness, and those that promote a stable macroeconomic environment. Interestingly, development aid appears to matter for the effect of productive capacities on economic resilience. On the one hand, the magnitude of the positive economic resilience effect of productive capacities increases as countries receive higher Aid for Trade (AfT) flows. On the other hand, NonAfT flows (i.e., other development aid flows that AfT flows) hinder the possible positive contribution of productive capacities to economic resilience. These findings have important policy implications that are discussed in the paper.
Green Approach to Develop Bee Pollen-loaded Alginate Based Nanocomposite Mat
Authors: Pakolpakçıl Ayben , Draczynski Zbigniew
Published: May 2021

Green electrospun materials are gaining popularity in the quest for a more sustainable environment for human life. Bee pollen (BP) is a valuable apitherapeutic product, which has many beneficial properties such as antioxidant properties and antibacterial activity. Alginate is a natural and low-cost polymer. Both natural materials show good compatibility with human tissues for biomedical applications and have no toxic effect on the environment. In this study, the production of bee pollen loaded sodium alginate (SA) and polyvinyl alcohol (PVA) nanofibrous mat was fabricated by electrospinning technique. The green electrospun composite mats were characterized by scanning electronic microscopy (SEM), Fourier transforms infrared spectroscopy (FTIR), and differential scanning calorimeter (DSC). According to the findings of the study, the toxin-free electrospinning method is suitable for producing green composite material. Because of the useful properties of the bee pollen and the favorable biocompatibility of the alginate fibers, the bee pollen-loaded SA/PVA electrospun mats have the potential for use in a variety of biomedical applications.
Literature Analysis of Artificial Intelligence in Biomedicine
Authors: Hulsen Tim
Published: May 2021

Artificial Intelligence (AI) refers to the simulation of human intelligence in machines, using machine learning, deep learning and neural networks. AI enables machines to learn from experience and perform human-like tasks. The field of AI research has been developing fast over the past five to ten years, due to the rise of ‘big data’ and increasing computing power. In the medical area, AI can be used to improve diagnosis, prognosis, treatment, surgery, drug discovery, or other applications. Therefore, both academia and industry are spending a lot of time and money in AI. This review takes a look at the biomedical literature (in the PubMed and Embase databases) and does some interesting observations: AI is growing exponentially over the past few years; it is used mostly for diagnosis; COVID-19 is already in the top-5 of diseases studied using AI; the United States, China, United Kingdom, South Korea and Canada are publishing the most articles in AI research; MIT is the world’s leading university in AI research; and convolutional neural networks are by far the most popular deep learning algorithms at this moment. The expectation is that AI will keep on growing, in spite of stricter privacy laws, more need for standardization, bias in the data, and the need for building trust. In order for AI to succeed in making healthcare better, it should be fully integrated into the clinician’s workflow so that they can focus on the contact with their patients.
Germanium and germanium-gold alloys under shock-wave loading
Authors: Maevskii Konstantin Konstantinovich
Published: May 2021

The results of numerical experiments upon modeling thermodynamic parameters such as value of pressure and compression of germanium and its alloys with gold are presented. The calculations were performed using the model TEC (thermodynamic equilibrium components). The model allows us to take into account the phase transition of germanium under shock-wave action. The interest in investigating of the compressibility for such materials is related both to the possibility of creating materials with the necessary properties and to the properties of the materials themselves. The results of calculations are compared with the known experimental results of different authors. The value of pressure and compression for shock wave loading of pure germanium and alloys with germanium as a component of various compositions are calculated.
PERANAN MODAL SOSIAL BAGI PETANI MISKIN UNTUK MEMPERTAHANKAN KELANGSUNGAN HIDUP RUMAH TANGGA DI PEDESAAN NGAWI (STUDI KASUS DI DESA RANDUSONGO KECAMATAN GERIH KABUPATEN NGAWI PROVINSI JAWA TIMUR)
Authors: Saheb Saheb , Slamet Yulius , Zuber Ahmad
Published: May 2021

This study was a descriptive qualitative research aiming to describe the role of Social Capital for the poor farmer to sustain their household life. The subject of research consisted of poor farmers having a less-than-0.13 ha land conducted in RT 4 RW 6 Bulu II Hamlet, Randusongo Village, Gerih Subdistrict, Ngawi Regency, East Java Province.

This study was conducted using in-depth interview, observation, and library study techniques. Meanwhile, the sampling technique used was maximum variation sampling taken seven informants as the sample, consisting of four informants: poor old, very poor old, poor young, and very poor young farmers, as the case informant, while the key informant of research consisted of: elders of Randusongo Village, Carik/Secretary of Randusongo Village, and Head of Bulu II Hamlet. Key informant was also used as the instrument of validating the field data from the informant.

The case informants were selected based on the criterion specified by Statistical Central Agency (BPS), that was, those belonging to poor family, while key informants were the village elders considered as knowledgeable and understanding about the daily condition of poor farmers because they domiciled in the same hamlet, and the village apparatus considered as knowing the poor farmers’ condition because they often gave them service regarding Bantuan Langsung Tunai ((BLT= Cash Direct Grant) now called Bantuan Langsung Sementara Masyarakat (BLSM = Temporary Public Grant), Raskin (rice for poor people) and other services.

The result showed that bonding social capital played an important role for the poor farmers in sustaining their household life. It could be seen from those helping their poor close relatives in meeting their daily life needs. Bridging social capital is the strongest out of the three social capitals existing because many poor farmer household could survive because of their close neighbor’s help. Linking social capital did not play a role at al because such organization as PKK (Pendidikan Kesejahteraan Keluarga = Family Welfare Education) or organization at either RT or RW level in Randusongo village did not work thereby could not contribute to reinforcing social capital within the society.

 
Keywords : social capital, poor farmer, life sustainability
PERGESERAN RESIPROSITAS MASYARAKAT DESA (STUDI ETNOGRAFI PERGESERAN NILAI TENTANG SUMBANGAN PERKAWINAN DI MASYARAKAT BRONGSONGAN, DESA SIDOREJO, KECAMATAN BENDOSARI, KABUPATEN SUKOHARJO)
Authors: Masithoh Nuraeni Dewi , Wijaya Mahendra , Kartono Drajat Tri
Published: May 2021

The aim of this research is (1) to find out how the shift of wedding contribution reciprocity is in Brongsongan society, Sidorejo Village, Bendosari, Sukoharjo Regency, and (2) to find out the cause of reciprocity shift in wedding contribution in Brongsongan society, Sidorejo Village, Bendosari, Sukoharjo Regency. This study was taken place in Brongsongan Village, Bendosari, Sukoharjo Regency. The research strategy employed was ethnography study, aiming to get an in-depth meaning of social-cultural activities interrelationship. The data was collected through direct observation and in-depth interview.The result of research showed that the shift of wedding contribution in Brongsongan society could be seen from: form, way of contributing and contribution function. Contributing was no longer a part of mechanic solidarity, but a part of organic solidarity and various transactional activity in the objective of getting either material or social benefit.

 
Keywords: Reciprocity, Exchange, Wedding Contribution
CYBER PROSTITUTION: BERGESERNYA MASALAH SOSIAL KE DALAM RUANG VIRTUAL
Authors: Laksono Puji , Magfiraini Riska
Published: May 2021

The development of Internet technology with social networking has
formed a new society in a virtual form called Cyber Society. In addition to
providing for the good of human life, the impact of this discovery also
affects the dark side of human life. Social problems in the real world were
also penetrated into this virtual world. One of the social problems in the
real world that are emerging in the cyber-society is prostitution (cyber
Prostitution). The problem of prostitution into the real dark side of society,
are now beginning to shift to the cyber society. Prostitution itself in the real
world are categorized as immoral act. The practice of prostitution in the
real world, being blocked by the system of values and norms in society. But
in cyber space which is an anarchist society without social control,
prostitution can take place freely.

Keywords: Cyber Prostitution, Social Problems, Virtual Spaces.
KEMISKINAN DALAM PEMBANGUNAN
Authors: Solikatun Solikatun , Masruroh Yulia , Zuber Ahmad
Published: May 2021

Dalam pembukaan Undang-Undang Dasar 1945 telah tercantum tujuan
pembangunan nasional yaitu untuk mewujudkan suatu masyarakat adil dan
makmur, material, dan spiritual berdasarkan Pancasila, di dalam wadah
negara kesatuan republik Indonesia yang merdeka, berdaulat, dan bersatu,
dalam suasana perikehidupan bangsa yang damai, tentram, tertib, dan
dinamis, serta dalam lingkungan pergaulan hidup dunia yang merdeka,
bersahabat, tertib, dan damai. Namun Maraknya kegiatan dan perencanaan
pembangunan belum sepenuhnya mampu mensejahterakan bangsa dan
Negara. Pembangunan di berbagai sektor juga belum dapat menampung dan
memenuhi kebutuhan masyarakat. Dapat kita lihat bahwa hingga kini
masalah kemiskinan belum bisa di tanggulangi dengan baik. Bahkan
semakin maraknya pembangunan semakin menambah deret kemiskinan di
negeri ini. Ketidak sesuaian antara tujuan pembangunan dengan realita
yang terjadi di lapangan dapat menimbulkan berbagai masalah. Perencanaan
dan program pembanguan belum dapat menanggulangi kemiskinan di
Negara Indonesia. Selain itu munculnya berbagai faktor yang
mempengaruhi kegagalan penanggulangan kemiskinan. Karena itu
dibutuhkan strategi pembangunan yang tepat guna menanggulangi
kemiskinan di Negara Indonesia.

Keywords: Program Pembangunan, Kemiskinan.
KESETARAAN GENDER DALAM LINGKUP ORGANISASI MAHASISWA UNIVERSITAS SEBELAS MARET SURAKARTA TAHUN 2016
Authors: Fibrianto Alan Sigit
Published: May 2021

This research aims to find out about the realization of gender equality in the scope of student organizations Sebelas Maret University Surakarta in 2016. This research uses qualitative descriptive method with source triangulation as data validity. Data obtained through observation and interviews conducted in each student organization at Sebelas Maret University. The results show that from all organizations there is male domination as the leader of the organization, while women serve as secretaries, treasurer and members. The reality is that there is no gender equivalence and justice, and gender mainstreaming from every organization, so the position of women is still at number two after men.

Keywords: Equality; Gender; Student Organization. 

Abstrak

Penelitian ini bertujuan untuk mengetahui mengenai wujud kesetaraan gender di lingkup organisasi mahasiswa Universitas Sebelas Maret Surakarta tahun 2016. Penelitian ini menggunakan metode deskriptif kualitatif dengan triangulasi sumber sebagai validitas data. Data diperoleh melalui observasi dan wawancara yang dilakukan di masing-masing organisasi mahasiswa di Universitas Sebelas Maret. Hasil penelitian menunjukkan bahwa dari semua organisasi terdapat dominasi laki-laki sebagai pimpinan organisasi, sedangkan perempuan menjabat sebagai sekretaris, bendahara dan anggota. Realitanya belum ada kesetaraan dan keadilan gender (KKG) serta pengarusutamaan gender (PUG) dari setiap organisasi, sehingga posisi perempuan masih berada pada nomor dua setelah laki-laki.

Kata Kunci: Kesetaraan; Gender; Organisasi Mahasiswa.

 
GERAKAN SOSIAL BARU PADA MUSIK: STUDI ETNOGRAFI PADA BAND NAVICULA
Authors: Wibisono Gunawan , Kartono Drajat Tri
Published: May 2021

Popular music not only being understood as entertainment media. Through audiences, popular music can be used as social movement instrument. This qualitative research using ethnography study as its approach. The subject on this resrach is Navicula band. The result of this stusy show that, the Navicula’s habitus about Tri Hita Karana’s life as a Balinese: the harmony between God, human and nature.Navicula’s cutural capital about knowledge of music as media and strong awareness of environment problems, social capital show their collaboration with several social organization, economic capital comes form album sales, band’s accessories and their concerts. Symbolic capital depicted by the term that Navicula as green grunge gentlemen. Navicula’s New social movement through Navicula’s discography as their messages, Borneo Tour  as new paradigm on collective action, cultural rebellion reflection through Navicula’s merchandise and hearing responses as movement’s impact. The finding on this research is relevant Bourdieu’s theory to depictedabout social praxis systematicly.New media movement theory can be alliance with other theory because its flexible nature to adjust with social dynamic society today.

Keywords: New Social Movement, Music, Ethnography.

 

Abstrak

Musik popular tidak hanya dipahami sebagai media hiburan semata. Melalui khalayak ramai, music popular bias menjadi alat untuk gerakan sosial. Penelitian ini bersifat kualitatif dengan menggunakan studi etnografi. Subjek penelitian ini adalah band Navicula. Hasil penelitian menunjukkan bahwa habitus Navicula tentang kehidupan Tri Hita Karana sebagai orang Bali; hubungan harmoni antara Tuhan, manusia dan alam semesta. Modal budaya Navicula tentang pengetahuan tentang music sebagai media dan pengetahuan yang kuat tentang masalah lingkungan, modal sosial menunjukkan kerjasama dengan beberapa organisasi sosial, modal ekonomi berasal dari penjualan album, aksesoris band dan konser mereka. Modal simbolik digambarkan dengan istilah Navicula sebagai green grunge gentlemen. Ranah Navicula menjelaskan di ranah jejaring sosial. Gerakan sosial baru Navicula melalui diskografi Navicula sebagai pesan yang disampaikan, Borneo Tour sebagai paradigma baru aksi kolektif, refleksi pemberontakan kultural melalui merchandise Navicula dan responpen dengar sebagai efek gerakan. Temuan penelitian ini menyiratkan teori Bourdieu yang relevan untuk menggambarkan tentang praktik sosial secara sistematis. Teori Gerakan Sosial Baru bisa dielaborasi dengan teori lain karena fleksibel untuk menyesuaikan diri dengan dinamika sosial masyarakat hari ini.
Kata Kunci: Gerakan Sosial Baru, Musik, Etnografi.
REPRESENTASI PENDIDIKAN KARAKTER BERBASIS KEARIFAN LOKAL MELALUI PEMBELAJARAN MEMBATIK
Authors: Ghufronudin Ghufronudin , Zuber Ahmad , Demartoto Argyo
Published: May 2021

Globalization outcome on the decline of the student’s love at local wisdom so that, student’s lack understanding to the significance of the batik’s existence as a heritage which full of life’s wisdom value. The aim of this research is to analyze the character education based on local wisdom representationthrough making batik at Batik 2 Senior High School, Surakarta.The analysis unit in this qualitative research with case study approach is stakeholders of Batik 2 Senior High School, Surakarta that been selected by purposeive sampling. Data collecting by observation, in-depth interview and documentation. Data validation with source triangulation the these data being analyzed by interactive model.The result of this resarch is show that, through making batik lessons in the school can achieve the balance on student’s between their consience, supra-ritual and intellectual related conected with God through philosophy and symbol of batik. Making batik lessons which carried out in the classical way through theory as well as pracctice bring positive impact for student’s character building that fit local wisdom values.

Keywords:Batik, Local Wisdom, Character Building, Representation.

 

Abstrak

Globalisasi berdampak pada menurunnya kecintaan siswa pada kearifan lokal sehingga siswa kurang memahami makna eksistensi batik sebagai warisan budaya yang kaya nilai-nilai kebijaksanaan hidup.Penelitian ini bertujuan untuk menganalisis representasi pendidikan karakter berbasis kearifan lokal melalui pembelajaran membatikdi SMA Batik 2 Surakarta.Unit analisis penelitian kualitatif dengan pendekatanstudi kasus ini adalah stakeholders SMA Batik 2 Surakarta yang dipilih dengan purposive sampling.Teknik pengumpulan data dengan observasi, wawancara mendalam dan dokumentasi.Validitas data dengan triangulasi sumber lalu dianalisis dengan modelinteraktif.Hasil penelitian menunjukkan bahwa melalui pembelajaran membatik di sekolah dapat tercapai keseimbangan hati nurani, supra-ritual, dan intelektualitas siswa berkaitan dengan Tuhan melalui kandungan nilai falsafah dalam simbol batik.Pembelajaran membatik yang dilakukan secara klasikal melalui teori maupun praktik berdampak positif bagi pembentukan karakter siswasesuai dengan nilai-nilai kearifan lokal.
Kata Kunci: Batik,Kearifan Lokal, Pendidikan Karakter, Representasi.
KEBERLANJUTAN EKOLOGIS HUTAN DALAM KEARIFAN LOKAL PANGLIMA UTEUN PADA MASYARAKAT NAGAN RAYA PROVINSI ACEH
Authors: Chadijah Devi Intan
Published: May 2021

This study aims to know how society defines and understands about forest and also how the impact of the society's cultural behaviour changes (local wisdom) on ecology sustainability in Nagan Raya regency. Fenomenology qualitative was used in this study and the data was collected through deep interview. Purposive sampling was used to determine the subject of this study. The result of study showed that nowadays the society has different understanding from before. This difference lead to behaviour changes of Nagan Raya society in using the forest. This is affected by society's experience, norm, value, economic needs toward the woods. Thus, the changing behaviour toward the forest gived the positive and negative impact. In one hand, for short term period this could be the life resources for the society's need. however, on long term period, it lead to individualistic life. The decrease function of tradition collective wisdom peulara uteun  lead to forest damage.

Keywords: Ecology Sustainability, Forest,  Local Wisdom, Meaning.

 

Abstrak

Penelitian ini bertujuan untuk mengetahui masyarakat mendefinisikan dan memahami tentang hutan dan juga terkait dampak perubahan perilaku budaya masyarakat (kearifan lokal) terhadap kelestarian lingkungan di Kabupaten Nagan Raya. Fenomenologi kualitatif digunakan dalam penelitian ini dan data dikumpulkan melalui wawancara mendalam. Purposive sampling digunakan untuk menentukan subjek penelitian ini. Hasil penelitian menunjukkan bahwa saat ini masyarakat memiliki pemahaman yang berbeda dari sebelumnya. Perbedaan ini menyebabkan perubahan perilaku masyarakat Nagan Raya dalam memanfaatkan hutan. Hal ini dipengaruhi oleh pengalaman, norma, nilai, kebutuhan ekonomi masyarakat terhadap hutan. Dengan demikian, perubahan perilaku terhadap hutan memberi dampak positif dan negatif. Di satu sisi, untuk jangka pendek ini bisa menjadi sumber kehidupan bagi kebutuhan masyarakat. Namun, dalam jangka panjang, hal itu mengarah pada kehidupan individualistis. Penurunan fungsi tradisi kolektif kebijaksanaan peulara uteun menyebabkan kerusakan hutan.

Kata Kunci: Keberlanjutan Ekologis, Hutan, Kearifan Lokal, Pemahaman
Zajal al-Asīr fī Madḥ al-Shaykh Usman Al-Bashir, Qaṣīdah Nūnīyah li-Musa Kalim Al-Qali: Dirāsah Taḥlīlīyah Adabīyah
Authors: Kabir Saleh Muhammad , Shariff Zakir Alhaj , Abdullahi Muhammad Tukur
Published: May 2021

This study aims to study the life of the poet Musa Kalim Al-Qali and his eulogy poem. The method used in this study is the descriptive-analytical approach, where the researchers read the selected poem and then follow it with study and analysis. The poem chosen was Zajal al-Asīr which contains his praise to Sheikh Usman Al-Bashir. The results of this study indicate that (1) the beginning of the poem (maṭla‘ al-qaṣīdah) invites the reader to follow what he will say about the praised Sheikh; (2) good disposal (ḥusn al-takhalluṣ) of the poem, the poet proceeded directly with the topic, noting that he did not explore his poem with a prologue or propaganda introduction; (3) a good syllable (ḥusn al-maqṭa‘) in the poem, the last words upon which the poet stood was well tolerated and kept a pleasure in listening in the most eloquent expression and the most beautiful meaning; (4) the sincerity of emotion (ṣidq al-‘āṭifah), the poem was distinguished by the sincerity of emotion, the heat of feeling, and the acuity of feeling.
Arizona Bird Committee Report, 2018–2020 Records
Authors: Rosenberg Gary H. , Radamaker Kurt
Published: May 2021

From 2018 to 2020 the Arizona Bird Committee reviewed 195 reports and updated the Arizona bird list through 2020, adding two species—the White-throated Thrush (Turdus assimilis) and Clay-colored Thrush (Turdus grayi)—bringing the Arizona state list to 567 species in good standing.
Action Video Game Training improves Text Reading Accuracy, Rate and Comprehension in Children with Dyslexia: A Randomized Controlled Trial
Authors: Peters Jessica L. , Crewther Sheila G. , Murphy Melanie J. , Bavin Edith L.
Published: May 2021

Dynamic visual attention training using Action Video Games (AVGs) is a promising intervention for dyslexia. This study investigated the efficacy of five hours (10x30min) of AVG training in dyslexic children (aged 8–13) using ‘Fruit Ninja’, while exploring whether increasing attentional and eye movement demands enhanced AVG effectiveness. Regular (AVG-R; n = 22) and enhanced AVG training (AVG+; n = 23) were compared to a treatment-as-usual comparison group (n = 19) on reading, rapid naming, eye movements and visuo-temporal processing. Playing ‘Fruit Ninja’ for only five hours significantly improved reading accuracy, rate, comprehension and rapid naming of both AVG groups, compared to the comparison group, though increasing attentional demands did not enhance AVG efficacy. Participants whose low contrast magnocellular-temporal processing improved most following AVG training also showed significantly greater improvement in reading accuracy. The findings demonstrate a clear role for visual attention in reading and highlight the clinical applicability of AVGs as fun, engaging interventions for dyslexia.
Appropriate selection of exercise intensity in patients with interstitial lung disease
Authors: Vonbank Karin , Lehmann Antje , Bernitzky Dominik , Gysan Maximilian Robert , Simon Stefan , Krotka Pavla , Zwick Ralf Harun , Idzko Marco , Burtscher Martin
Published: May 2021

Background

Training intensities in pulmonary rehabilitation are commonly based on fixed percentages of peak heart rate (HRpeak), heart rate reserve (HRR), or peak work load (Wpeak). For patients suffering from interstitial lung disease (ILD) it is unknown, whether those intensities are appropriate when compared to individual ventilatory thresholds (VT1 and VT2) derived from cardiopulmonary exercise testing (CPET). The aim of this study was to compare fixed HR percentages with HRs at VT1 and VT2.
Study Design and Methods

A total of 120 subjects, 80 ILD patients and 40 healthy controls, underwent a symptom-limited CPET. From the ILD patient, 32 suffered from idiopathic pulmonary fibrosis (IPF), 37 from connective tissue disease (CTD) and 11 from sarcoidosis.
Results

HRs at fixed percentages, i.e., at 70%HRpeak, at 70%Wpeak and at 60%HRR were significantly lower in the ILD patients compared to the control group. Large percentages of HR values at 70%Wpeak and 60%HRR ranged between the HRs at VT1 and VT2 in ILD subgroups and controls as well. HRs at 70%HRpeak were lower than HRs at VT1 in 66% of the IPF patients, 54% of the CTD patients and 55% of patients with sarcoidosis compared to 18% in the control group.
Discussion

Our findings demonstrate a considerable scattering of fixed HR percentages compared to HRs at the individual VTs derived from CPET in ILD patients. Thus, the use of HRs at the individual VTs is recommended for appropriate prescription of exercise intensity in pulmonary rehabilitation of ILD patients.
NEBL and AKT1 Maybe New Targets to Eliminate the Colorectal Cancer Cells Resistance to Oncolytic Effect of Vesicular Stomatitis Virus
Authors: Mamizadeh Zoleikha , kalani Mohamad Reza , Parsania Masoud , Dallal Mohamad Mehdi Soltan , Moradi Abdolvahab
Published: May 2021

Background: Oncolytic virotherapy is a promising therapeutic approach for several cancers which however has been reported that is ineffective in some cases of the same cancer. A well-known oncolytic virus is the Vesicular stomatitis virus (VSV) due to activating the apoptosis mechanism in tumor cells through its Matrix (M) protein. This study compares the different invasive intensity Colorectal tumors for the oncolytic effect of VSV wild type (wt) and M51R M-protein.Methods: 114 fresh colorectal tumor primary cell cultures plus SW480 and HCT116 colorectal cancer cell lines were examined. Fresh tumor samples were divided into two groups of lower stages (I/II) and higher stages (III/IV) regarding the medical records. The presence of two mutations in the PIK3CA gene, and the expression of NEBL and AKT1 genes were evaluated to biologically compare the staging classification. The cell lines and the stable primary tissue cultures were transfected with a plasmid encoding VSV wild type and M51R mutant M-protein. Western blotting assay confirmed the expression of the protein. Results: MTT assay results showed either wild type or M51R mutant can kill SW480 and stage I/II primary cultures while mutant M-protein had no apoptotic effects on HCT116 cells and stage III/IV primary cultures. Morphological apoptosis features were observed by a phase-contrast microscope. NEBL and AKT1 expression were significantly higher in resistant cells. Elevated Caspase 9 activity confirmed that the intrinsic apoptosis pathway is the reason for cell death in SW480 cells. The apoptosis rate was quantified and reconfirmed by Annexin V FITC/PI multicolor flow-cytometry. Conclusions: Different tumors from the same cancer exhibit different sensitivity to M51R M-proteins due to genetic difference. NEBL and AKT1 gene expression may be responsible for this difference which maybe the target of future investigations. Therefore, tumor staging should be considered in oncolytic viral treatment as an interfering factor.
Characterization of the vaginal microbiota of women with premature ovarian insufficiency
Authors: Wen Jingyi , Feng Yanzhi , Yuan Suzhen , Yan Wei , Zhang Jinjin , Luo Aiyue , Wang Shixuan
Published: May 2021

Background: Premature ovarian insufficiency (POI) is a complex reproductive endocrine disease that can affect multiple systems. It is highly heterogeneous in etiology and the exact etiology remains unclear. Infectious factors may be related to POI, but researches on the alterations of microbiome in POI patients are scarce.
Results: Vaginal swabs were collected from 52 POI patients (29 for sequencing, 23 for validation) and 46 healthy individuals of comparable age (26 for sequencing, 20 for validation). 16S rRNA gene sequencing targeting the V3-V4 hypervariable regions was performed to evaluate the alterations of vaginal microbiota in POI patients. The relative abundance of Actinobacteria (23.34% vs 10.65%, P=0.017), Atopobium (11.11% vs 0.01%, P﹤0.001), and Gardnerella (8.05% vs 3.14%, P=0.002) were significantly increased in POI patients, while Bifidobacterium (3.95% vs 7.44%, P=0.017) was significantly decreased. Cluster analysis of dominant strains showed that the proportion of POI patients whose predominant bacteria were not Lactobacillus was increased than the control group, but the difference was not statistically significant (8/29 vs 2/26, P=0.105). More interestingly, these changes in vaginal microbiota were significantly correlated with declined ovarian function in POI patients, including decreased ovarian reserve, ovarian endocrine disruption, and symptoms of perimenopausal syndrome. Actinobacteria, Atopobium, and Gardnerella appeared to be detrimental to ovarian function, while Bifidobacterium seemed to be benificial.
Conclusions: The present study revealed the correlation between vaginal microbiota and POI, fill the gap in the field of microbial and POI research and provide a new research strategy for POI.
Gut microbiota derived metabolites 3-idoleacetic acid with LPS induces generation of IL-35+B cells
Authors: Yang rongcun , Su Xiaomin , qi Houbao , Zhang Minying , Gao Yunhuan , Yang Yazheng , Yun Huan , Zhang Qianjing , Yang Xiaorong , Zhang Yuan , He Jiangshan , Fan Yaqi , wang Yuxue , Guo Pei , Zhang Chunze
Published: May 2021

Background

IL-35 producing B and Treg cells are critical regulators in worldwide chronic illnesses, which are related to disruptions of gut microbiota composition. Whether gut microbiota regulate these IL-35+ cells maintains elusive. Here we investigated the regulation of gut microbiota in the IL-35+ cells through genetically modified mouse models against obesity.
Results

Here, we first found that gut Reg4 can promote resistance to high-fat diet induced obesity. Using 16S rRNA gene-based microbiota analysis combined with LC-MS (Liquid chromatography-mass spectrometry)/MS, we demonstrate that gut Reg4 associated microbiota such as lactobacillus can promote generation of the IL-35+ B cells through producing 3-idoleacetic acid (IAA) in the presence of LPS. HuREG4IECtg mice had accumulation of IL-35+ cells not only in adipose but also in colon after feeding high-fat diet; whereas decreased IL-35+ cells in adipose and colon tissues could be detected in Reg4 knockout (KO) mice. Lower levels of IAA were also detected in the peripheral blood of individuals with obesity. Mechanistically, IAA with LPS mediated IL35+ B cells are through PXR and TLR4. PXR KO or TLR4 KO impaired generation of IL-35+B cells.
Conclusion

IAA with LPS can induce generation of the IL-35+B cells through PXR and TLR4.
Circ-Epc1 in Adipose-Derived Stem Cell Exosomes Can Improve Cognition by Shifting Microglial M1/M2 Polarization in Alzheimer’s Disease Mice Model
Authors: Liu Haining , Jin Mingming , Ji Minxiu , Zhang Wei , Liu An , Wang Tao
Published: May 2021

Background: Alzheimer’s disease (AD) is the most major dementia in the globe. More evidence informs that exosomes from adipose-derived stem cells (ADSCs) could therapeutically affect cognitive function in AD-associated pathophysiology. However, their role and regulatory mechanism remain largely unknown. Methods: High-throughput sequencing was used to identify differentially expressed exosomal circRNAs from ADSCs or hypoxia pretreated ADSCs. Luciferase reporter assays and RT-qPCR were used to investigate the relationships between circ-Epc1, miR-770-3p, and TREM2. APP/PS1 double transgenic AD model mice were then utilized to study therapeutic effect regarding circ-Epc1 in ADSCs exosomes. BV2 cells were used to understand the regulatory relationship between circ-Epc1, miR-770-3p, and TREM2 and how these interactions modulated phenotypic transformation and inflammatory cytokine expression in microglia. The result show that exosomes from hypoxia pretreatment ADSCs had a greater therapeutic effect at improving cognitive function by decreasing neuronal damage in the hippocampus. Results: High-throughput sequencing found that circ-Epc1 played an important role in hypoxia pretreated ADSC exosomes regarding their ability to improve cognitive function. Luciferase reporter assays showed that TREM2 and miR-770-3p were downstream targets of circ-Epc1. Overexpressing miR-770-3p or downregulating TREM2 reversed the effects of circ-Epc1 on M2 microglia under LPS treatment. In vivo experiments showed that circ-Epc1-containing ADSC exosomes increased the therapeutic effect of exosome at improving cognitive function by decreasing neuronal damage and shifting hippocampal microglia from M1 to M2 polarization. Conclusion: Taken together, the data found that circ-Epc1 was highly expressed in ADSC exosomes and improved cognition by shifting microglial M1/M2 polarization in AD mouse model.
Influence of CO2 migration from geological storage on the chemical composition of groundwater and monitoring indicators
Authors: Li Lian , Wu Yahong , Chong Shan , Wen Qingzhi , Lin Qianguo , Shuo Zhang
Published: May 2021

In the process of CO2 geological sequestration, there is a risk that CO2 will migrate to and dissolve in groundwater, resulting in a series of physical and chemical reactions, with influence on chemical compositions of groundwater. In this paper, numerical simulations are conducted to study the influence of CO2 migration on the chemical composition of groundwater. The modeling results show that when CO2 migrates to the groundwater, gaseous CO2 will migrate upward and diffuse laterally under the effect of buoyancy. The acidity of groundwater is enhanced and the pH is significantly reduced, leading to dissolution of calcite, illite, oligoclase, K-feldspar, chlorite and hematite, and precipitation of quartz, kaolinite, smectite-Na, siderite and smectite-Ca. The porosity of the formation increases. The concentrations of K+ and Fe2+ in groundwater gradually increase, the concentrations of Ca2+ and HCO3− basically remain unchanged after increasing to a certain value, the concentrations of Mg2+ and AlO2− first increase and then decrease, and the concentrations of Na+, Cl− and SO42− first decrease slightly and then gradually increase, but the change in concentration is small. Therefore, Fe2+, Mg2+, Ca2+, K+ and pH can be used as important monitoring indicators of whether CO2 migrates to groundwater during geological storage.
Diagnostic and Prognostic Value of microRNAs for Environmental Exposure to Asbestiform Fibers Causing the Malignant Mesothelioma
Authors: Filetti Veronica , Loreto Carla , Falzone Luca , Lombardo Claudia L , Cannizzaro Emanuele , Castorina Sergio , Ledda Caterina , Rapisarda Venerando
Published: May 2021

BackgroundFluoro-edenite (FE) is an asbestiform fiber identified in the lavic products of Monte Calvario from stone quarries located in the southeast of Biancavilla (Sicily, Italy). Environmental exposure to this silicate has been associated with a higher incidence of Malignant Mesothelioma (MM), highly aggressive cancer that affects the pleural tissue. Median survival for MM was 9.8 months, with 5% of patients surviving at 5 years. At present, several diagnostic biomarkers have been evaluated for MM and many studies demonstrated that microRNAs (miRNAs) have the potential to be considered as good non-invasive diagnostic and prognostic biomarkers for cancer.A set of miRNAs involved in MM was previously identified after a careful in silico selection. Starting from these preliminary data, this study aimed to validate the predicted diagnostic significance of hsa-miR-323a-3p, hsa-miR-101-3p, and hsa-miR-20b-5p on a subset of MM patients exposed to FE and matched healthy controls. For this purpose, MM tissues vs. nonmalignant pleura tissues were analyzed through droplet digital PCR to evaluate differences in the expression levels of these miRNAs and their MM diagnostic potential. In addition, computational analysis have been performed to establish the correlation of these miRNAs with the online available asbestos exposure data and clinic-pathological parameters to verify a potential role of these miRNAs as prognostic tools. The interaction between these miRNAs and the main genes mutated and altered in MM was evaluated.ResultsDroplet digital PCR results showed that the three analyzed miRNAs were significantly down-regulated in MM cases vs. controls. ROC analysis revealed high specificity and sensitivity rates for both hsa-miR-323a-3p, and hsa-miR-20b-5p, which thus acquire a diagnostic value for MM. In silico results showed a potential prognostic role of hsa-miR-101-3p due to a significant association of its higher expression and increased Overall Survivor of MM patients. Finally, the computational analysis have showed that these miRNAs can target and modulate both oncogene and tumor suppressor genes.ConclusionsThese miRNAs should be further evaluated not only as diagnostic tools, but also as prognostic predictors. Furthermore, the validation of their targets and regulators would clarify how miRNAs induce or repress critical pathways involved in the carcinogenesis triggered by carcinogenic fibers exposure.