bluuebunny/crossref_metadata_2025 huggingface dataset:
THEORY OF FRACTIONAL IMPLICIT DIFFERENTIAL EQUATIONS WITH COMPLEX ORDER
Authors: ELSAYED Elsayed , VİVEK D. , KANAGARAJAN K.
Published: December 2019

In this paper, we consider boundary value problems for the following nonlinear implicit differential equations with complex order D +x(t) = f t,x(t),D +x(t) , ? = m+i?, t ? J := [0,T],  ax(0)+bx(T) = c,where D + is the Caputo fractional derivative of order ? ? C. Let ? ? R , 0 < ? < 1, m ? (0,1], and f : J ×R ? R is given continuous function. Here a,b,c are real constants with a+b  = 0. We derive the existence and stability of solution for a class of boundary value problem(BVP) for nonlinear fractional implicit differential equations(FIDEs) with complex order. The results are based upon the Banach contraction principle and Schaefer’s fixed point theorem.
Hubungan antara Perkembangan Psikososial Remaja dengan Perilaku Bullying di SMAN 1 Tolitoli
Authors: Pangaribuan Helena , Arifuddin Arifuddin , Lenny Lenny
Published: December 2019

Adolescence is the phase of the transition periode in the age range of 11-20 years. Bullying behavior in adolescents is aggressive behavior that is done intentionally and occurs repeatedly to attack a target or victim who is weak, insulted and unable to defend himself, can have a negative impact on individual life, academic life, social life for perpetrators, victims and witnesses of bullying, if it is not properly addressed the risk of negative developmental behavior of adolescent behavior can affect the next stage of growth and development. This study aims to look at the relationship between the psychosocial development of adolescents and bullying behavior at SMAN I Tolitoli. Methodology: The design in this study was quantitative with 148 samples using the Proportionate Stratified Random Sampling with a cross sectional approach tested using chis-square. The results showed that 30.4% of adolescents had normal psychosocial development and 69.6% of adolescents had abnormal psychosocial development, students with behavior that carried out bullying behavior were 59.5% and students with behavior that did not conduct bullying behavior were 40.5%. Conclusion: there is a relationship between the psychosocial development of adolescents and the bullying behavior of adolescents at SMAN I Tolitoli. The results obtained p value of 0.016, the value is smaller than the alpha value of 0.05. It is hoped that for students to increase the creativity of adolescents, parents and schools provide health education about the dangers of bullying behavior.
Discovery of Novel Doxorubicin Metabolites in MCF7 Doxorubicin-Resistant Cells
Authors: Wang Xu , Hui Renjie , Chen Yun , Wang Wentao , Chen Yujiao , Gong Xiaohai , Jin Jian
Published: December 2019

Doxorubicin (DOX) is metabolized to a variety of metabolitesin vivo, which has been shown to be associated with cardiotoxicity. We speculate that metabolic processes are also present in tumor cells. A LC-MS/MS method was developed to detect intracellular metabolites. Drug resistant tumor cells with high drug stress tolerance and metabolically active are suitable as materials for this study. Our results show difference in drug metabolites between the wild-type and drug-resistant cells. Three novel doxorubicin metabolites were discovered after the LC-MS/MS analysis. All these metabolites and their profiles of metabolites are totally different from that in liver or kidneyin vivo. Our results suggest that tumor cells and drug-resistant tumor cells have a unique drug metabolism pathway for doxorubicin.
Erratum: Van Loon, J., et al. Precision for Smallholder Farmers: A Small-Scale-Tailored Variable Rate Fertilizer Application Kit. Agriculture, 2018, 8, 48
Authors: Van Loon Jelle , Speratti Alicia B. , Gabarra Louis , Govaerts Bram
Published: December 2019

The authors wish to correct the following erratum in this paper [...]
Modelling and Validation of Computer Vision Techniques to Assess Heart Rate, Eye Temperature, Ear-Base Temperature and Respiration Rate in Cattle
Authors: Jorquera-Chavez Maria , Fuentes Sigfredo , Dunshea Frank R. , Warner Robyn D. , Poblete Tomas , Jongman Ellen C.
Published: December 2019

Precision livestock farming has emerged with the aim of providing detailed information to detect and reduce problems related to animal management. This study aimed to develop and validate computer vision techniques to track required features of cattle face and to remotely assess eye temperature, ear-base temperature, respiration rate, and heart rate in cattle. Ten dairy cows were recorded during six handling procedures across two consecutive days using thermal infrared cameras and RGB (red, green, blue) video cameras. Simultaneously, core body temperature, respiration rate and heart rate were measured using more conventional ‘invasive’ methods to be compared with the data obtained with the proposed algorithms. The feature tracking algorithm, developed to improve image processing, showed an accuracy between 92% and 95% when tracking different areas of the face of cows. The results of this study also show correlation coefficients up to 0.99 between temperature measures obtained invasively and those obtained remotely, with the highest values achieved when the analysis was performed within individual cows. In the case of respiration rate, a positive correlation (r = 0.87) was found between visual observations and the analysis of non-radiometric infrared videos. Low to high correlation coefficients were found between the heart rates (0.09–0.99) obtained from attached monitors and from the proposed method. Furthermore, camera location and the area analysed appear to have a relevant impact on the performance of the proposed techniques. This study shows positive outcomes from the proposed computer vision techniques when measuring physiological parameters. Further research is needed to automate and improve these techniques to measure physiological changes in farm animals considering their individual characteristics.
A Functional 3′ UTR Polymorphism of FADS2 Affects Cow Milk Composition through Modifying Mir-744 Binding
Authors: Li Mingxun , Lu Xubin , Gao Qisong , Wang Mengqi , Arbab Abdelaziz Adam Idriss , Sun Yujia , Chen Zhi , Zhang Huimin , Karrow Niel A. , Yang Zhangping , Mao Yongjiang
Published: December 2019

This study determined the associations of FADS2 c.1571G>A with milk FAs content and revealed that cows with the GG genotype had improved levels of delta-6 desaturase substrates (linoleic acid, C18:2n-6; p < 0.001) and decreased levels of desaturase products (gamma-linolenic acid, C18:3n-6; p < 0.001), indicating a reduction in FADS2 expression or delta-6 desaturase activity caused by this polymorphism. Computer alignment demonstrated that c.1571G>A occurred within a potential miR-744 binding site. When the c.1571G allele was present, the luciferase activity of reporter constructs was significantly suppressed by miR-744, while no such effect was observed with the A allele. Overexpression of miR-744 in bovine mammary epithelial cells (with the 1571GG genotype) downregulated FADS2 expression at both mRNA and protein levels. In contrast, inhibition of endogenous miR-744 with a specific inhibitor dramatically upregulated FADS2 expression. Taken together, these lines of evidence indicated that the c.1571A minor allele abolished the ability of miR-744 to bind FADS2, with a consequent increase in FADS2 expression levels and synthesis of omega-6 LC-PUFAs.
Using Principles from Applied Behaviour Analysis to Address an Undesired Behaviour: Functional Analysis and Treatment of Jumping Up in Companion Dogs
Authors: Pfaller-Sadovsky Nicole , Arnott Gareth , Hurtado-Parrado Camilo
Published: December 2019

The aim of this study was to investigate the feasibility and effectiveness of procedures successfully used in human related applied behaviour analysis practices to the field of clinical animal behaviour. Experiment 1 involved functional analyses to identify the reinforcement contingencies maintaining jumping up behaviour in five dogs. Experiment 2 comprised teaching dog owners a noncontingent reinforcement intervention (i.e., time-based reinforcement) via behavioural skills training. Single-case experimental methods were implemented in both experiments. The results of Experiment 1 showed that access to a tangible (dogs D01, D02, D03, and D04) and owner attention (dog D05) were reliably maintaining the jumping up behaviour. Experiment 2 demonstrated that noncontingent reinforcement effectively reduced jumping in three out of four dogs (Tau −0.59, CI 90% [−1–0.15], p = 0.026, Tau −1, CI 90% [−1–−0.55], p = 0.0003, and Tau −0.32, CI 90% [−0.76–0.11], p = 0.22 for dyads D01, D02, and D05, respectively), and that behavioural skills training was successful in teaching owners to perform a dog training intervention with high fidelity. Although the results are promising, more canine-related research into functional analysis and noncontingent reinforcement, as well as implementation of behavioural skills training with animal caregivers, is needed.
Convergence Analysis of Capacities for Photovoltaics and Energy Storage System Considering Energy Self-Sufficiency Rates and Load Patterns of Rural Areas
Authors: Cho Choon Young , Lee Wonbin , Bhang Byeong Gwan , Choi Jinho , Lee Sang Hun , Woo Sung Cheol , Ahn Hyung Keun
Published: December 2019

This work is a case study of 905 households, to present methods for optimizing the capacity of photovoltaic sytems (PVs)/energy storage systems (ESSs) for household to reach a desired energy self-sufficiency (70% to 90%). In order to calculate the capacity of PV and ESSs that would enhance the energy self-sufficiency of families in rural areas, the solar radiance data of the target area for the last five years and the average monthly power usage in the previous year were collected. Then, households with an average energy consumption of 250 kWh per month were chosen for this research. According to the simulation done using Solar Pro, the optimized capacities of PVs and ESSs are 2.67 kW and 7.15 kW, respectively, in order to achieve 90% energy self-sufficiency. We visualized the change in the optimum capacity of PVs and ESSs for the desired energy self-sufficiency. This study would be the base work for forming a grid-distributed energy network system by expanding the system to a national scale.
Experimental Study on Geysers Induced by the Release of Trapped Air in Storage Tunnel Systems
Authors: Wang Xiaosheng , Qian Shangtuo , Chen Hongxun
Published: December 2019

A storage tunnel system is the critical infrastructure of urban drainage systems, in which the rapid filling of water and release of trapped air can lead to the “geyser” phenomenon. This may cause serious damage, threatening both system operation and personal safety. In this paper, a detailed experimental study was carried out based on synchronous recorded video images and digital image processing technology. According to experimental observations, gas-flow geysers and surge-type geysers were analyzed deeply. The former is caused by high-speed gas flow and is accompanied by a pressure drop; the latter is caused by surge pressure and is accompanied by a pressure increase. The free surface velocity of the gas-flow geyser is mainly affected by the external pressure, the air volume, the diameter of the shaft, and the height of the water column, and the geyser phenomenon cannot occur when the air column is mainly dominated by buoyancy. Based on dimensional analysis and data fitting, this paper presents the empirical formula for the free surface velocity and the interface net velocity and puts forward the critical occurrence conditions for the gas-flow geyser.
Study of the Compression Behavior of Steel-Fiber Reinforced Concrete by Means of the Response Surface Methodology
Authors: de la Rosa Ángel , Ruiz Gonzalo , Poveda Elisa
Published: December 2019

The compression behavior of steel-fiber reinforced concrete (SFRC) has been addressed exhaustively in recent decades thereby highlighting a variety of differences with regard to the effect that the addition of fiber has on it. In this paper, a detailed study of the subject is developed for which a database has been created, which includes 197 tests performed on cylindrical concrete specimens with dimensions of 150 × 300 mm 2 (diameter × height). By means of the response surface methodology, we disclose the relationship that exists between the geometric parameters of the fiber (length, diameter, and aspect ratio), their amount (fraction in volume), and some matrix parameters (compression resistance and maximum size of coarse aggregate) with the different compression responses of the SFRC, which are strength, elastic modulus, critical deformation under maximum load, and the volumetric deformation work in the pre- and post-peak branch. Linear polynomial models are chosen to adjust each response with the defined factors, and said variables are studied in a dimensional and non-dimensional format. From the results obtained, it is verified how the inclusion of steel-fibers produces notable improvements in ductility and the energy absorption capacity of the concrete when significantly increasing the works of volumetric deformation in the pre- and post-peak branch with respect to the matrix without fibers. In addition, a new model is analyzed, which describes the stress–strain curve of the compression behavior of the SFRC based on the increase of ductility and energy absorption. This model is characterized by a softening branch subsequent to the peak load determined by means of the residual compressive strength, a parameter that corresponds to the value of the compressive stress associated with a strain equal to three times that of the peak of the curve, which is significantly dependent on the aspect ratio and fiber content.
A Three-Class Classification of Cognitive Workload Based on EEG Spectral Data
Authors: Plechawska-Wójcik Małgorzata , Tokovarov Mikhail , Kaczorowska Monika , Zapała Dariusz
Published: December 2019

Evaluation of cognitive workload finds its application in many areas, from educational program assessment through professional driver health examination to monitoring the mental state of people carrying out jobs of high responsibility, such as pilots or airline traffic dispatchers. Estimation of multilevel cognitive workload is a task usually realized in a subject-dependent way, while the present research is focused on developing the procedure of subject-independent evaluation of cognitive workload level. The aim of the paper is to estimate cognitive workload level in accordance with subject-independent approach, applying classical machine learning methods combined with feature selection techniques. The procedure of data acquisition was based on registering the EEG signal of the person performing arithmetical tasks divided into six intervals of advancement. The analysis included the stages of preprocessing, feature extraction, and selection, while the final step covered multiclass classification performed with several models. The results discussed show high maximal accuracies achieved: ~91% for both the validation dataset and for the cross-validation approach for kNN model.
Low Kinetic Energy Oxygen Ion Irradiation of Vertically Aligned Carbon Nanotubes
Authors: Acosta Selene , Casanova Chafer Juan , Sierra Castillo Ayrton , Llobet Eduard , Snyders Rony , Colomer Jean-François , Quintana Mildred , Ewels Chris , Bittencourt Carla
Published: December 2019

Vertically aligned multiwalled carbon nanotubes (v-CNTs) were functionalized with oxygen groups using low kinetic energy oxygen ion irradiation. X-ray photoelectron spectroscopy (XPS) analysis indicates that oxygen ion irradiation produces three different types of oxygen functional groups at the CNTs surface: epoxide, carbonyl and carboxyl groups. The relative concentration of these groups depends on the parameters used for oxygen ion irradiation. Scanning electron microscopy (SEM) shows that the macroscopic structure and alignment of v-CNTS are not affected by the ion irradiation and transmission electron microscopy (TEM) proves tip functionalization of v-CNTs. We observed that in comparison to oxygen plasma treatment, oxygen ion irradiation shows higher functionalization efficiency and versatility. Ion irradiation leads to higher amount of oxygen grafting at the v-CNTs surface, besides different functional groups and their relative concentration can be tuned varying the irradiation parameters.
Near-Infrared Quartz-Enhanced Photoacoustic Sensor for H2S Detection in Biogas
Authors: Zhao Fagang , Gao Yutong , Yang Lin , Yan Yuqing , Li Jiashi , Ren Jingrong , dello Russo Stefano , Zifarelli Andrea , Patimisco Pietro , Wu Hongpeng , Dong Lei
Published: December 2019

A quartz-enhanced photoacoustic spectroscopy (QEPAS) sensor for H2S detection operating in near-infrared spectral range is reported. The optical source is an erbium-doped fiber amplified laser with watt-level optical power. The QEPAS spectrophone is composed of a quartz tuning fork with a resonance frequency of 7.2 kHz, a quality factor of 8500, and a distance between prongs of 800 µm, and two tubes with a radius of 1.3 mm and a length of 23 mm acting as an organ pipe resonator. With this spectrophone geometry, the photothermal noise contribution of the spectrophone was removed and the theoretical thermal noise level was achieved. The position of both tubes with respect to custom quartz tuning fork has been investigated as a function of signal amplitude, Q-factor, and noise of the QEPAS sensor when a high-power laser was used. Benefit from the linearity of the QEPAS signal to the excitation laser power, a detection sensitivity of 330 ppb for H2S detection was achieved at atmospheric pressure and room temperature, when the laser power was 1.6 W and the signal integration time was set to 300 ms, corresponding to a normalized noise equivalent absorption of 3.15 × 10−9 W cm−1/(Hz)1/2. The QEPAS sensor was then validated by measuring H2S in a biogas sample.
Maximum UV Index Records (2010–2014) in Quito (Ecuador) and Its Trend Inferred from Remote Sensing Data (1979–2018)
Authors: Parra René , Cadena Eliana , Flores Camila
Published: December 2019

To prevent adverse health effects, the World Health Organization promotes the diffusion of the ultraviolet radiation index (UVI), with messages promoting precautionary behaviors, through a scale that considers extreme UVI values to be larger than 11.0. This scale came from a proposal from Canada, a country with a mostly light-skinned population, which experiences maximum UVI values up to 10.0. A modified scale was proposed, adapted to the skin types and the UVI levels in South America, which considers extreme values larger than 16.0. The records from 2010 to 2014 indicated that UVI is frequently larger than 11.0 (40.0–76.1% of the days per month) in Quito (Ecuador). The number of days per month with levels larger than 16.0 varied between 0.7% and 32.0%. We found that the maximum UV index levels do not occur necessarily around the local solar noontime. As the basis for a self-warning system in Quito and based on their skin type and UVI levels, people should know the exposure time before damage can take place. The Tropospheric Emission Monitoring Internet Service (TEMIS) computed the UVI at local solar noontime and under clear-sky conditions. The records from 2010 to 2014 were congruent with the corresponding TEMIS values. We did not identify any trend of the daily TEMIS UVI values during 1979 to 2018, which, used as a proxy, suggested the real UVI levels in Quito during 2010 to 2018 varied in a range similar to 1979–2009.
Observational and Modelling Study of a Major Downburst Event in Liguria: The 14 October 2016 Case
Authors: Parodi Antonio , Lagasio Martina , Maugeri Maurizio , Turato Barbara , Gallus William
Published: December 2019

Downbursts are very disruptive weather events that can produce large amounts of damage. The most studied downbursts are those occurring in the United States and continental Europe, but they can happen globally. This work is an observational and modelling analysis of a major downburst event that occurred on 14 October 2016 over eastern Liguria (Italy). This downburst affected an area 30 km long and 10 km wide, producing observed wind gusts of 40 m/s with major impacts to railways, trees, and houses, with more than 2.5 million euros of damage. First, the general environment influencing this downburst is identified and analyzed, then the event is reproduced with a small multi-physics high-resolution ensemble using the Weather Research and Forecasting (WRF)–advanced research WRF (ARW) model, with 1 km horizontal grid spacing. The event was poorly predicted beforehand, and the difficulty in forecasting this event is confirmed by the fact that so few ensemble members suggested the occurrence of damaging winds over eastern Liguria. However, one of the eight members performed well and its output helped to reveal the primary mechanisms for the downburst, suggesting that high-resolution ensembles using mixed physics may be a useful tool for improving the prediction of similar extreme events in the Mediterranean region in the future.
Anticonvulsant Essential Oils and Their Relationship with Oxidative Stress in Epilepsy
Authors: da Fonsêca Diogo Vilar , da Silva Maia Bezerra Filho Carlos , Lima Tamires Cardoso , de Almeida Reinaldo Nóbrega , de Sousa Damião Pergentino
Published: December 2019

Epilepsy is a most disabling neurological disorder affecting all age groups. Among the various mechanisms that may result in epilepsy, neuronal hyperexcitability and oxidative injury produced by an excessive formation of free radicals may play a role in the development of this pathology. Therefore, new treatment approaches are needed to address resistant conditions that do not respond fully to current antiepileptic drugs. This paper reviews studies on the anticonvulsant activities of essential oils and their chemical constituents. Data from studies published from January 2011 to December 2018 was selected from the PubMed database for examination. The bioactivity of 19 essential oils and 16 constituents is described. Apiaceae and Lamiaceae were the most promising botanical families due to the largest number of reports about plant species from these families that produce anticonvulsant essential oils. Among the evaluated compounds, β-caryophyllene, borneol, eugenol and nerolidol were the constituents that presented antioxidant properties related to anticonvulsant action. These data show the potential of these natural products as health promoting agents and use against various types of seizure disorders. Their properties on oxidative stress may contribute to the control of this neurological condition. However, further studies on the toxicological profile and mechanism of action of essential oils are needed.
Benzyl Isothiocyanate Induces Apoptosis via Reactive Oxygen Species-Initiated Mitochondrial Dysfunction and DR4 and DR5 Death Receptor Activation in Gastric Adenocarcinoma Cells
Authors: Han Khin Wah Wah , Po Wah Wah , Sohn Uy Dong , Kim Hyun-Jung
Published: December 2019

Benzyl isothiocyanate (BITC) is known to inhibit the metastasis of gastric cancer cells but further studies are needed to confirm its chemotherapeutic potential against gastric cancer. In this study, we observed cell shrinkage and morphological changes in one of the gastric adenocarcinoma cell lines, the AGS cells, after BITC treatment. We performed 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, a cell viability assay, and found that BITC decreased AGS cell viability. Reactive oxygen species (ROS) analyses using 2′,7′-dichlorofluorescin diacetate (DCFDA) revealed that BITC-induced cell death involved intracellular ROS production, which resulted in mitochondrial dysfunction. Additionally, cell viability was partially restored when BITC-treated AGS cells were preincubated with glutathione (GSH). Western blotting indicated that BITC regulated the expressions of the mitochondria-mediated apoptosis signaling molecules, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and cytochrome c (Cyt c). In addition, BITC increased death receptor DR5 expression, and activated the cysteine-aspartic proteases (caspases) cascade. Overall, our results showed that BITC triggers apoptosis in AGS cells via the apoptotic pathways involved in ROS-promoted mitochondrial dysfunction and death receptor activation.
Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach
Authors: Crossley Lisa , Attoye Bukola , Vezza Vincent , Blair Ewen , Corrigan Damion , Hannah Stuart
Published: December 2019

We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open-circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and noncomplementary DNA target sequences.
Mother Schema, Obstetric Dilemma, and the Origin of Behavioral Modernity
Authors: Parncutt Richard
Published: December 2019

What triggered the emergence of uniquely human behaviors (language, religion, music) some 100,000 years ago? A non-circular, speculative theory based on the mother-infant relationship is presented. Infant “cuteness” evokes the infant schema and motivates nurturing; the analogous mother schema (MS) is a multimodal representation of the carer from the fetal/infant perspective, motivating fearless trust. Prenatal MS organizes auditory, proprioceptive, and biochemical stimuli (voice, heartbeat, footsteps, digestion, body movements, biochemicals) that depend on maternal physical/emotional state. In human evolution, bipedalism and encephalization led to earlier births and more fragile infants. Cognitively more advanced infants survived by better communicating with and motivating (manipulating) mothers and carers. The ability to link arbitrary sound patterns to complex meanings improved (proto-language). Later in life, MS and associated emotions were triggered in ritual settings by repetitive sounds and movements (early song, chant, rhythm, dance), subdued light, dull auditory timbre, psychoactive substances, unusual tastes/smells and postures, and/or a feeling of enclosure. Operant conditioning can explain why such actions were repeated. Reflective consciousness emerged as infant-mother dyads playfully explored intentionality (theory of mind, agent detection) and carers predicted and prevented fatal infant accidents (mental time travel). The theory is consistent with cross-cultural commonalities in altered states (out-of-body, possessing, floating, fusing), spiritual beings (large, moving, powerful, emotional, wise, loving), and reports of strong musical experiences and divine encounters. Evidence is circumstantial and cumulative; falsification is problematic.
Sport Activity as Risk or Protective Factor in Feeding and Eating Disorder
Authors: Iuso Salvatore , Bellomo Antonello , Pagano Tiziana , Carnevale Raffaella , Ventriglio Antonio , Petito Annamaria
Published: December 2019

Emerging evidence suggests controversial results on the associations between sport activity and eating disorders (EDs). The aim of the present study was to investigate the relationship between sport activity in general, weight-dependent/independent sport activity particularly, and risk or protective factors in feeding and eating disorder (FED). The sample (n = 282, divided into two successive groups), included competitive athletes in the first analysis, non-competitive athletes, and sedentary peers; in the second analysis it has been divided into weight-dependent athletes, weight-independent athletes, non-competitive athletes, and sedentary peers. The participants were tested with Rosenberg Self-Esteem Scale, Profile of Mood State (POMS) questionnaire, Body Shape Questionnaire (BSQ), Eating Attitudes Test (EAT-26), and Body Uneasiness Test (BUT). The results show higher levels of self-esteem among athletes in general and particularly in weight-independent athletes (p = 0.0210). We found higher levels of image and body dissatisfaction among sedentary peers and weight-dependent athletes (0.0005 < p < 0.0015). Sedentary peers also reported higher levels of tension/anxiety, depression/dejection, confusion/bewilderment and fatigue/inertia (0.0001 < p < 0.0331). Dieting and oral control were found to be higher among weight-dependent athletes (0.0337 < p < 0.0400). The findings suggest that sedentary condition is associated with higher levels of body-image discomfort and higher level of psychological distress, whereas weight-dependent athletes may report dietary issues and bodily concerns. Sport activity should be promoted and specific trainings on diet and body-consciousness encouraged among athletes.
Notch Signaling Molecules as Prognostic Biomarkers for Acute Myeloid Leukemia
Authors: Takam Kamga Paul , Collo Giada Dal , Resci Federica , Bazzoni Riccardo , Mercuri Angela , Quaglia Francesca Maria , Tanasi Ilaria , Delfino Pietro , Visco Carlo , Bonifacio Massimiliano , Krampera Mauro
Published: December 2019

The role of Notch signaling in acute myeloid leukemia (AML) is still under investigation. We have previously shown that high levels of Notch receptors and ligands could interfere with drug response. In this study, the protein expression of 79 AML blast samples collected from newly diagnosed patients was examined through flow cytometry. Gamma-secretase inhibitors were used in AML mouse xenograft models to evaluate the contribution of Notch pharmacological inhibition to mouse survival. We used univariate analysis for testing the correlation and/or association between protein expression and well-known prognostics markers. All the four receptors (Notch1–4) and some ligands (Jagged2, DLL-3) were highly expressed in less mature subtypes (M0–M1). Notch3, Notch4, and Jagged2 were overexpressed in an adverse cytogenetic risk group compared to good cytogenetic risk patients. Chi-square analysis revealed a positive association between the complete remission rate after induction therapy and weak expression of Notch2 and Notch3. We also found an association between low levels of Notch4 and Jagged2 and three-year remission following allogeneic stem cell transplantation (HSCT). Accordingly, Kaplan–Meier analysis showed improved OS for patients lacking significant expression of Notch4, Jagged2, and DLL3. In vivo experiments in an AML mouse model highlighted both improved survival and a significant reduction of leukemia cell burden in the bone marrow of mice treated with the combination of Notch pan-inhibitors (GSIs) plus chemotherapy (Ara-C). Our results suggest that Notch can be useful as a prognostic marker and therapeutic target in AML.
Carbon Quantum Dots (CQDs) Decorated Bi2O3-x Hybrid Photocatalysts with Promising NIR-Light-Driven Photodegradation Activity for AO7
Authors: Xian Tao , Sun Xiaofeng , Di Lijing , Zhou Yongjie , Ma Jun , Li Hongqin , Yang Hua
Published: December 2019

In this work, Bi2O3-x with surface oxygen vacancies was prepared through the NaBH4 reduction of Bi2O3. After that, carbon quantum dots (CQDs) were deposited onto the surface of the Bi2O3-x to obtain a series of the CQDs/Bi2O3-x composites. The HRTEM and XPS characterizations of the CQDs/Bi2O3-x composites suggest that the thickness of surface oxygen vacancies could be adjusted by changing the concentration of NaBH4 solution, and the intimate contact between CQDs and the Bi2O3-x is achieved. Acid orange 7 (AO7) was adopted as the target reactant for investigating the photocatalytic degradation activities of the CQDs/Bi2O3-x composites under simulated sunlight and NIR light irradiation. It is found that the photocatalytic activities of the samples are closely related to the concentration of NaBH4 and content of CQDs. The Bi2O3-x samples exhibit enhanced simulated-sunlight-driven photocatalytic activity compared with Bi2O3. Specifically, the optimal degradation efficiency of AO7 is achieved over the 3R-Bi2O3-x (concentration of NaBH4: 3 mmol/L), which is 1.38 times higher than the degradation AO7 efficiency over Bi2O3. After the decoration of the 3R-Bi2O3-x surface with CQDs, the simulated-sunlight-driven photocatalytic activity of the CQDs/Bi2O3-x composite could be further enhanced. Among the samples, the 15C/3R-Bi2O3-x sample reveals the highest photocatalytic activity, leading to an AO7 degradation percentage of ~97% after 60 min irradiation. Different from Bi2O3 and the 3R-Bi2O3-x, the 15C/3R-Bi2O3-x sample also exhibits near-infrared (NIR)-light-driven photocatalytic degradation activity. In addition, the intrinsic photocatalytic activity of CQDs/Bi2O3-x composite was further confirmed by the degradation of phenol under simulated sunlight and NIR light irradiation. The photocurrent response and electrochemical impedance spectroscopy (EIS) measurements confirm the efficient migration and separation of photogenerated charges in the CQDs/Bi2O3-x samples. The •OH and h+ are proved to be the main reactive species in the simulated sunlight and NIR light photocatalytic processes over the CQDs/Bi2O3-x composites. According to the above experiments, the photocatalytic degradation mechanisms of the CQDs/Bi2O3-x composites under simulated sunlight and NIR light illumination were proposed.
Biodiesel at the Crossroads: A Critical Review
Authors: Estevez Rafael , Aguado-Deblas Laura , Bautista Felipa M. , Luna Diego , Luna Carlos , Calero Juan , Posadillo Alejandro , Romero Antonio A.
Published: December 2019

The delay in the energy transition, focused in the replacement of fossil diesel with biodiesel, is mainly caused by the need of reducing the costs associated to the transesterification reaction of vegetable oils with methanol. This reaction, on an industrial scale, presents several problems associated with the glycerol generated during the process. The costs to eliminate this glycerol have to be added to the implicit cost of using seed oil as raw material. Recently, several alternative methods to convert vegetable oils into high quality diesel fuels, which avoid the glycerol generation, are being under development, such as Gliperol, DMC-Biod, or Ecodiesel. Besides, there are renewable diesel fuels known as “green diesel”, obtained by several catalytic processes (cracking or pyrolysis, hydrodeoxygenation and hydrotreating) of vegetable oils and which exhibit a lot of similarities with fossil fuels. Likewise, it has also been addressed as a novel strategy, the use of straight vegetable oils in blends with various plant-based sources such as alcohols, vegetable oils, and several organic compounds that are renewable and biodegradable. These plant-based sources are capable of achieving the effective reduction of the viscosity of the blends, allowing their use in combustion ignition engines. The aim of this review is to evaluate the real possibilities that conventional biodiesel has in order to success as the main biofuel for the energy transition, as well as the use of alternative biofuels that can take part in the energy transition in a successful way.
In-Situ Catalytic Fast Pyrolysis of Pinecone over HY Catalysts
Authors: Jeong Jaehun , Lee Hyung Won , Jang Seong Ho , Ryu Sumin , Kim Young-Min , Park Rae-su , Jung Sang-Chul , Jeon Jong-Ki , Park Young-Kwon
Published: December 2019

The in-situ catalytic fast pyrolysis of pinecone over HY catalysts, HY(30; SiO2/Al2O3), HY(60), and 1% Ni/HY(30), was studied by TGA and Py-GC/MS. Thermal and catalytic TGA indicated that the main decomposition temperature region of pinecone, from 200 to 400 °C, was not changed using HY catalysts. On the other hand, the DTG peak heights were differentiated by the additional use of HY catalysts. Py-GC/MS analysis showed that the efficient conversion of phenols and other oxygenates formed from the pyrolysis of pinecone to aromatic hydrocarbons could be achieved using HY catalysts. Of the HY catalysts assessed, HY(30), showed higher efficiency in the production of aromatic hydrocarbons than HY(60) because of its higher acidity. The aromatic hydrocarbon production was increased further by increasing the pyrolysis temperature from 500 to 600 °C and increasing the amount of catalyst due to the enhanced cracking ability and overall acidity. The use of 1% Ni/HY(30) also increased the amount of monoaromatic hydrocarbons compared to the use of HY(30) due to the additional role of Ni in enhancing the deoxygenation and aromatization of reaction intermediates.
Genipin as An Emergent Tool in the Design of Biocatalysts: Mechanism of Reaction and Applications
Authors: Tacias-Pascacio Veymar G. , García-Parra Esmeralda , Vela-Gutiérrez Gilber , Virgen-Ortiz Jose J. , Berenguer-Murcia Ángel , Alcántara Andrés R. , Fernandez-Lafuente Roberto
Published: December 2019

Genipin is a reagent isolated from the Gardenia jasminoides fruit extract, and whose low toxicity and good crosslinking properties have converted it into a reactive whose popularity is increasing by the day. These properties have made it widely used in many medical applications, mainly in the production of chitosan materials (crosslinked by this reactive), biological scaffolds for tissue engineering, and nanoparticles of chitosan and nanogels of proteins for controlled drug delivery, the genipin crosslinking being a key point to strengthen the stability of these materials. This review is focused on the mechanism of reaction of this reagent and its use in the design of biocatalysts, where genipin plays a double role, as a support activating agent and as inter- or intramolecular crosslinker. Its low toxicity makes this compound an ideal alterative to glutaraldehyde in these processes. Moreover, in some cases the features of the biocatalysts prepared using genipin surpassed those of the biocatalysts prepared using other standard crosslinkers, even disregarding toxicity. In this way, genipin is a very promising reagent in the design of biocatalysts.
Mapping Research in the Obesity, Adipose Tissue, and MicroRNA Field: A Bibliometric Analysis
Authors: Manoel Alves João , Handerson Gomes Teles Ramon , do Valle Gomes Gatto Camila , Muñoz Vitor Rosetto , Regina Cominetti Márcia , Garcia de Oliveira Duarte Ana Cláudia
Published: December 2019

Recent studies have investigated the control of adipose tissue expansion and inflammatory process by microRNAs (miRNAs). These two processes are of great interest because both are associated with obesity and metabolic syndrome. However, despite the great relevance of the role of miRNAs in obesity and adipose tissue, no qualitative and quantitative analysis on the subject has been performed. Thus, we aimed to examine global research activity and current trends with respect to the interaction between obesity, adipose tissue and miRNAs through a bibliometric analysis. This research was performed on the Scopus database for publications containing miRNA, obesity, and adipose tissue keyword combinations. In total, 898 articles were analyzed and the most frequently occurring keywords were selected and clustered into three well-defined groups. As a result, first group of keywords pointed to the research area on miRNAs expressed in obesity-associated diseases. The second group demonstrated the regulation of the adipogenesis process by miRNAs, while the third group highlighted brown adipose tissue and thermogenesis as one of the latest global research trends related to the theme. The studies selected in this paper describe the expression and performance of different miRNAs in obesity and comorbidities. Most studies have focused on identifying miRNAs and signaling pathways associated with obesity, type 2 diabetes mellitus, and cardiovascular disease. Thus, the miRNA profile for these diseases may be used as biomarkers and therapeutic targets in the prevention and treatment of obesity-associated diseases.
B-RAFV600E Inhibitor Dabrafenib Attenuates RIPK3-Mediated Necroptosis and Promotes Functional Recovery after Spinal Cord Injury
Authors: Sugaya Takehiro , Kanno Haruo , Matsuda Michiharu , Handa Kyoichi , Tateda Satoshi , Murakami Taishi , Ozawa Hiroshi , Itoi Eiji
Published: December 2019

The receptor-interacting protein kinase 3 (RIPK3) is a key regulator of necroptosis and is involved in various pathologies of human diseases. We previously reported that RIPK3 expression is upregulated in various neural cells at the lesions and necroptosis contributed to secondary neural tissue damage after spinal cord injury (SCI). Interestingly, recent studies have shown that the B-RAFV600E inhibitor dabrafenib has a function to selectively inhibit RIPK3 and prevents necroptosis in various disease models. In the present study, using a mouse model of thoracic spinal cord contusion injury, we demonstrate that dabrafenib administration in the acute phase significantly inhibites RIPK3-mediated necroptosis in the injured spinal cord. The administration of dabrafenib attenuated secondary neural tissue damage, such as demyelination, neuronal loss, and axonal damage, following SCI. Importantly, the neuroprotective effect of dabrafenib dramatically improved the recovery of locomotor and sensory functions after SCI. Furthermore, the electrophysiological assessment of the injured spinal cord objectively confirmed that the functional recovery was enhanced by dabrafenib. These findings suggest that the B-RAFV600E inhibitor dabrafenib attenuates RIPK3-mediated necroptosis to provide a neuroprotective effect and promotes functional recovery after SCI. The administration of dabrafenib may be a novel therapeutic strategy for treating patients with SCI in the future.
Visualization of Sirtuin 4 Distribution between Mitochondria and the Nucleus, Based on Bimolecular Fluorescence Self-Complementation
Authors: Ramadani-Muja Jeta , Gottschalk Benjamin , Pfeil Katharina , Burgstaller Sandra , Rauter Thomas , Bischof Helmut , Waldeck-Weiermair Markus , Bugger Heiko , Graier Wolfgang F. , Malli Roland
Published: December 2019

Mitochondrial sirtuins (Sirts) control important cellular processes related to stress. Despite their regulatory importance, however, the dynamics and subcellular distributions of Sirts remain debatable. Here, we investigate the subcellular localization of sirtuin 4 (Sirt4), a sirtuin variant with a mitochondrial targeting sequence (MTS), by expressing Sirt4 fused to the superfolder green fluorescent protein (Sirt4-sfGFP) in HeLa and pancreatic β-cells. Super resolution fluorescence microscopy revealed the trapping of Sirt4-sfGFP to the outer mitochondrial membrane (OMM), possibly due to slow mitochondrial import kinetics. In many cells, Sirt4-sfGFP was also present within the cytosol and nucleus. Moreover, the expression of Sirt4-sfGFP induced mitochondrial swelling in HeLa cells. In order to bypass these effects, we applied the self-complementing split fluorescent protein (FP) technology and developed mito-STAR (mitochondrial sirtuin 4 tripartite abundance reporter), a tripartite probe for the visualization of Sirt4 distribution between mitochondria and the nucleus in single cells. The application of mito-STAR proved the importation of Sirt4 into the mitochondrial matrix and demonstrated its localization in the nucleus under mitochondrial stress conditions. Moreover, our findings highlight that the self-complementation of split FP is a powerful technique to study protein import efficiency in distinct cellular organelles.
H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis
Authors: Lin Wenfang , Huang Dongmei , Shi Ximiao , Deng Ban , Ren Yujun , Lin Wenxiong , Miao Ying
Published: December 2019

Leaf senescence, either as a natural stage of development or as an induced process under stress conditions, incorporates multiple intricate signaling pathways. At the cellular level, retrograde signals have been considered as important players during the initiation and progression of senescence in both animals and plants. The plant-specific single-strand DNA-binding protein WHIRLY1 (WHY1), a repressor of leaf natural senescence, is dually located in both nucleus and plastids. Despite many years of studies, the myth about its dual location and the underlying functional implications remain elusive. Here, we provide further evidence in Arabidopsis showing that alteration in WHY1 allocation between the nucleus and chloroplast causes perturbation in H2O2 homeostasis, resulting in adverse plant senescence phenotypes. The knockout of WHY1 increased H2O2 content at 37 days post-germination, coincident with an early leaf senescence phenotype, which can be rescued by ectopic expression of the nuclear isoform (nWHY1), but not by the plastid isoform (pWHY1). Instead, accumulated pWHY1 greatly provoked H2O2 in cells. On the other hand, exogenous H2O2 treatment induced a substantial plastid accumulation of WHY1 proteins and at the same time reduced the nuclear isoforms. This H2O2-induced loss of nucleus WHY1 isoform was accompanied by enhanced enrichments of histone H3 lysine 9 acetylation (H3K9ac) and recruitment of RNA polymerase II (RNAP II) globally, and specifically at the promoter of the senescence-related transcription factor WRKY53, which in turn activated WRKY53 transcription and led to a senescence phenotype. Thus, the distribution of WHY1 organelle isoforms and the feedback of H2O2 intervene in a circularly integrated regulatory network during plant senescence in Arabidopsis.
A Membrane Permeable Prodrug of S223 for Selective Epac2 Activation in Living Cells
Authors: Xu Yunjian , Schwede Frank , Wienk Hans , Tengholm Anders , Rehmann Holger
Published: December 2019

Signalling by cyclic adenosine monophosphate (cAMP) occurs via various effector proteins, notably protein kinase A and the guanine nucleotide exchange factors Epac1 and Epac2. These proteins are activated by cAMP binding to conserved cyclic nucleotide binding domains. The specific roles of the effector proteins in various processes in different types of cells are still not well defined, but investigations have been facilitated by the development of cyclic nucleotide analogues with distinct selectivity profiles towards a single effector protein. A remaining challenge in the development of such analogues is the poor membrane permeability of nucleotides, which limits their applicability in intact living cells. Here, we report the synthesis and characterisation of S223-AM, a cAMP analogue designed as an acetoxymethyl ester prodrug to overcome limitations of permeability. Using total internal reflection imaging with various fluorescent reporters, we show that S223-AM selectively activates Epac2, but not Epac1 or protein kinase A, in intact insulin-secreting β-cells, and that this effect was associated with pronounced activation of the small G-protein Rap. A comparison of the effects of different cAMP analogues in pancreatic islet cells deficient in Epac1 and Epac2 demonstrates that cAMP-dependent Rap activity at the β-cell plasma membrane is exclusively dependent on Epac2. With its excellent selectivity and permeability properties, S223-AM should get broad utility in investigations of cAMP effector involvement in many different types of cells.