bluuebunny/crossref_metadata_2025 huggingface dataset:
Mental health help-seeking barriers, facilitators and interventions. A systematic review
Authors: Aguirre Antonia , Silva Ignacio , Billings Jo , Jimenez Magdalena , Rowe Sarah
Published: September 2019

Background: Increasing rates of mental health problems among adolescents are alarming. Teens who are most in need of mental health attention are reluctant to seek help. A better understanding of the help-seeking in this population is crucial to overcome this gap. Methods: Five databases were searched to identify the principal barriers, facilitators and interventions targeting help-seeking for common mental health problems in adolescents aged 10-19 years. The search was performed in June 2018 and updated in March 2019. Two independent screening processes were made using the eligibility criteria. Quality assessment of each study was performed and findings summarised using a narrative synthesis. Results: 90 studies meet the inclusion criteria for this review for barrier and facilitators (n=54) and interventions (n=36). Stigma and negative beliefs towards mental health services and professionals were the most cited barriers. Facilitators included previous positive experience with health services and mental health literacy. Most interventions were based on psychoeducation, which focused on general mental health knowledge, suicide and self-harm, stigma and depression. Other types of interventions included the use of multimedia and online tools, peer training and outreach initiatives. Overall, the quality of studies was low to medium and there was no general agreement regarding help-seeking definition and measurements. Conclusion: Most of interventions took place in an educational setting however, it is important to consider adolescents outside the educational system. Encouraging help-seeking should come with the increased availability of mental health support for all adolescents in need, but this is still a major challenge for Child and Adolescent Mental Health Services. There is also a need to develop shared definitions, theoretical frameworks and higher methodological standards in research regarding help-seeking behaviours in adolescents. This will allow more consistency and generalisability of findings, improving the development of help-seeking interventions and ensuring timely access to mental health treatments.
Opposite roles of BAP1 in overall survival of uveal melanoma and cutaneous melanoma
Authors: Liu-Smith Feng
Published: September 2019

Background: BAP1 germline mutations predispose individuals to a number of cancer types including uveal melanoma (UM) and cutaneous melanoma (CM) which are distinctively different in the oncogenic pathways. BAP1 loss was common in UM and was associated with a worse prognosis. BAP1 loss was rare in CM and the outcome was unclear. Methods: This study used TCGA UM and CM databases for survival analysis for patients with different BAP1 status and mRNA expression levels. Cox regression model was used for adjusting to known prognosis factors. Results: BAP1- (loss or low expression) predicted a poor overall survival in UM (Cox HR = 0.062, logrank p =0.007) but a contrasting better overall survival in CM (HR = 1.69, p =0.009). Multi-covariate Cox regression analysis indicated BAP1 was a significant predictor for overall survival after adjusting for age of diagnosis, presence of ulceration, Breslow depth and CM stages in patients older than 50 years but not in younger patients. Co-expression analysis revealed no shared genes in BAP1 altered UM and CM tumors, further supporting a completely distinctive role of BAP1 in CM and UM. Conclusions: low BAP1 mRNA was significantly associated with a better overall survival in CM patients, in sharp contrast to its tumor suppressor role in UM where low or loss of BAP1 indicated a worse overall survival. Function of BAP1 may be dependent on cellular context.
Signatures of selection for resistance to Haemonchus contortus in sheep and goats
Authors: Estrada-Reyes Zaira Magdalena , Tsukahara Yoko , Amadeu Rodrigo R. , Goetsch Arthur L. , Gipson Terry A. , Sahlu Tilahun , Puchala Richard , Wang Zaisen , Hart Steve P. , Mateescu Raluca G.
Published: September 2019

Background: Gastrointestinal nematode infection (GNI) is the most important disease affecting the small ruminant industry in U.S. The environmental conditions in the southern United States are ideal for the survival of the most pathogenic gastrointestinal nematode, Haemonchus contortus . Host genetic variation for resistance to H. contortus allows selective breeding for increased resistance of animals. This selection process increases the prevalence of particular alleles in sheep and goats and creates unique genetic patterns in the genome of these species. The aim of this study was to identify loci with divergent allelic frequencies in a candidate gene panel of 100 genes using two different approaches (frequentist and Bayesian) to estimate F st outliers in three different breeds of sheep and goats exposed to H. contortus .

Results: Our results for sheep populations showed SNPs under selection in C3AR1 , CSF3 , SOCS2 , NOS2 , STAT5B , TGFB2 and IL2RA genes using frequentist and Bayesian approaches. For goats, SNPs in CD1D , ITGA9 , IL12A , IL13RA1, CD86 and TGFB2 genes were under selection. Common signatures of selection in both species were observed in NOS2 , TGFB2 and TLR4 genes. Directional selection was present in all SNPs evaluated in the present study.

Conclusions: A total of 13 SNPs within 10 genes of our candidate gene panel related to H. contortus exposure were identified under selection in sheep populations. For goats, 11 SNPs within 7 genes were identified under selection. Results from this study support the hypothesis that resistance to H. contortus is likely to be controlled by many loci. Shared signatures of selection related to mechanisms of immune protection against H. contortus infection in sheep and goats could be useful targets in breeding programs aimed to produce resistant animals with low FEC.
Genome-wide identification and transcriptome profiling reveal that E3 ubiquitin ligase genes relevant to ethylene, auxin and abscisic acid are differentially expressed in the fruits of melting flesh and stony hard peach varieties
Authors: Tan Bin , Lian Xiaodong , Cheng Jun , Zeng Wenfang , Zheng Xianbo , Wang Wei , Ye Xia , Li Jidong , Li Zhiqian , Zhang Langlang , Feng Jiancan
Published: September 2019

Background: Ubiquitin ligases (E3) are the enzymes in the ubiquitin/26S proteasome pathway responsible for targeting proteins to the degradation pathway and play major roles in multiple biological activities. However, the E3 family is still yet to be identified, and the functions of E3 ligase genes on fruit flesh are unknown in peach.
Results: In this study, genome-wide identification, classification and characterization of the E3 ligase genes within the genome of peach (Prunus persica) was carried out. In total, 765 E3 (PpE3) ligase genes were identified in the peach genome. The PpE3 ligase genes were divided into eight subfamilies according to the presence of known functional domains. The RBX subfamily was not detected in peach. The PpE3 ligase genes were not randomly distributed among the 8 chromosomes, with a greater concentration on the longer chromosomes. The primary mode of gene duplication of the PpE3 ligase genes was dispersed gene duplication (DSD). Four subgroups of the BTB subfamily never characterized before were newly identified in peach, namely BTBAND, BTBBL, BTBP and BTBAN. The expression patterns of the identified E3 ligase genes in two peach varieties that display different types of fruit softening (melting flesh, MF, and stony hard, SH) were analyzed at 4 different stages of ripening using Illumina technology. Among the 765 PpE3 ligase genes, 515 (67.3%) were expressed (FPKM >1) in the fruit of either MF or SH during fruit ripening. In same-stage comparisons, 231 differentially expressed genes (DEGs) were identified between the two peach cultivars. The number of DEGs in each subfamily varied. Most DEGs were members of the BTB, F-box, U-box and RING subfamilies. PpE3 ligase genes predicted to be involved in ethylene, auxin, or ABA synthesis or signaling and DNA methylation were differentially regulated. Eight PpE3 ligase genes with possible roles in peach flesh texture and fruit ripening were discussed.
Conclusions: The results of this study provide useful information for further understanding the functional roles of the ubiquitin ligase genes in peach. The findings also provide the first clues that E3 ligase genes may function in the regulation of peach ripening.
Constructing an Experiential Education Model in Undergraduate Radiology Education by the Utilization of the Picture Archiving and Communication System (PACS)
Authors: Chen Yingqian , Zheng Keguo , Ye Shanshan , Wang Jifei , Xu Ling , Li Ziping , Meng Quanfei , Yang Jianyong , Feng Shiting
Published: September 2019

Background
Medical education in China is in a transitional period changing from passive learning to experiential education models. We have modified an experiential education method for radiology education. The aim of this study is to evaluate the effect of this method on undergraduate radiology education.
Methods
With the help of the picture archiving and communication system (PACS) and RadiAnt DICOM Viewer, we have modified an experiential education method that simulates similar working conditions for undergraduate medical students to take on the role as clinical radiologists and formulate radiology diagnosis. A total of 101 students were allocated into either the experiential education group or the control group. The final examination scores and a 5-point Likert scale self-assessment questionnaire of radiologic skills were collected from all the students as an objective assessment and a subjective assessment respectively. A questionnaire was also used to assess the satisfaction with the experiential model in the experiential education group. Mann-Whitney U test was used to compare the ranked data, and t -tests were used to compare the numeric data.
Results
The experiential education group demonstrated significantly higher scores (7.4±1.3) in the short answer type questions on imaging “description and diagnosis” when compared to the control group (6.7±1.5, p <0.05). The self-assessment questionnaire indicated that the experiential education corresponded to an increased familiarity with the diagnostic principles, the imaging sequences and reconstruction methods of computer tomography (CT), which also strengthen participants’ confidence to perform future clinical work (p <0.05). The self-assessment questionnaire from the experiential education group showed great satisfaction with the organization (82.5%), interactivity (85%) and quality (85%) of the learning activity in majority of students. Most students found this model of learning to be helpful for studying radiology (85%) and for understanding anatomy (90%).
Conclusion
Compared with the traditional radiology education approach, the experiential education model showed greater efficacy in improving students’ analytical and diagnostic skills as well as clinical confidence.
Field and greenhouse evaluations of soil suppressiveness to Heterodera glycines in the Midwest corn-soybean production systems
Authors: Hu Weiming , Kidane Eyob , Neher Deborah A. , Chen Senyu
Published: September 2019

Soil suppressive to the soybean cyst nematode (SCN), a major yield-limiting pathogen of soybean, plays an important role in biological control. Field and greenhouse experiments were conducted to evaluate the effects of tillage, crop sequence, and biocide application on SCN suppression in corn-soybean cropping systems in Minnesota. The experiment was a split-plot design with no-tillage and conventional tillage as main plots, and six crop-biocide treatments (CRCS, CSCS, SSSS, SSSS + streptomycin, SSSS + captan, and SSSS + formaldehyde – the four letters represent crops in 2009 to 2012, respectively; C is corn, R is SCN-resistant soybean, and S is SCN-susceptible soybean) as subplots with four replicates. Soil samples were taken from each plot at planting, midseason, and harvest each year for SCN egg counts, and soybean yield was determined. In addition, soil samples collected from each plot at midseason were assayed for suppressiveness to SCN. Tillage had minimal effect on SCN population density and soybean yield. Annual rotation with corn reduced SCN population density, but also reduced soil suppressiveness as SCN egg population density increased in the following SCN-susceptible soybean compared with soybean monoculture. Rotation with SCN-resistant soybean and corn was the most effective in reducing SCN population density. The bactericide streptomycin did not affect SCN populations but the fungicide captan increased SCN population density. The biocide formaldehyde was the most effective in reducing the level of suppressiveness to SCN. The greenhouse study confirmed that the soil was suppressive to SCN, but failed to detect effects of tillage, crop sequence, and biocide field treatments. This study demonstrated that the soil in the fields was suppressive to the SCN, and biological agents, especially fungal antagonists, were involved in nematode suppression.
The Draft Genome of Deladenus siricidicola
Authors: Postma Alisa , Mlonyeni X. Osmond , Clasen Frederick , Joubert Fourie , Slippers Bernard
Published: September 2019

The nematode Deladenus siricidicola is used as biological control agent against the invasive woodwasp Sirex noctilio, a serious invasive pest of Pinus plantations globally. The draft genome of this ecologically and economically important entomoparasitic nematode was determined.
Integrative taxonomy of Xiphinema histriae and Xiphinema lapidosum from Spain
Authors: Cai Ruihang , Archidona-Yuste Antonio , Cantalapiedra-Navarrete Carolina , Palomares-Rius Juan E. , Zheng Jingwu , Castillo Pablo
Published: September 2019

Three populations of Xiphinema non-americanum group species were detected in agricultural and natural ecosystems, during routine surveys for plant-parasitic nematodes in Spain. Based on morphological and molecular analyses, the species were identified as Xiphinema histriae and Xiphinema lapidosum, being this the first record and molecular characterization of both species in Spain. The morphometrics and morphology of the Spanish populations agree with those of the original description and paratype specimens and the present study provided a first description of the second to fourth juvenile stages of both species. A detailed study on the morphology in the Spanish populations of X. histriae, as well as in paratypes, showed a pseudo-Z-organ with weakly muscularized wall and containing numerous small dense granular bodies, which was different to the original description by Lamberti et al. (1993). This new finding suggests that X. histriae must be considered a member of the morphospecies Group 5 of X. non-americanum. Phylogenetic analysis based on D2 to D3 expansion segments of 28S gene, ITS1 and partial CoxI gene indicated that X. histriae and X. lapidosum are phylogenetically related with other Xiphinema non-americanum group spp. reported from Spain. Considering the pathological and economic importance of this group of nematodes, the combination of morphological characters, measurements, and molecular analysis is crucial for accurate identification of these species.
Screening of Cucurbita maxima and Cucurbita moschata Genotypes for Resistance Against Meloidogyne arenaria, M. incognita, M. javanica, and M. luci
Authors: Aydınlı Gökhan , Kurtar Ertan Sait , Mennan Sevilhan
Published: September 2019

The host response of fifteen winter squash (Cucurbita maxima) and five pumpkin (Cucurbita moschata) dihaploid genotypes to Meloidogyne arenaria, M. incognita, M. javanica, and M. luci was screened in pot experiments. Root galling and nematode reproduction were detected in all combinations of plant genotype and nematode species. Ten genotypes of C. maxima and three genotypes of C. moschata were considered highly resistant (<10% of the susceptible genotype) or moderately resistant (<50% of the susceptible genotype) to one or more Meloidogyne species based on nematode reproduction as a percentage of the most susceptible genotype. Genotypes 55CA15-A3 and G14-IP1 of C. maxima were highly resistant to M. luci and M. arenaria, respectively. Both 14BO01-O2 and G9-A4 genotypes of C. moschata were considered highly resistant to M. arenaria. However, these genotypes still allowed significant nematode reproduction because egg number per plant was higher than initial number of eggs used as inoculum, indicating that all genotypes were hosts.
First Report of the Root-Knot Nematode, Meloidogyne floridensis Infecting Guardian® Peach Rootstock in South Carolina, USA
Authors: Reighard Gregory L. , Henderson William G. , Scott Sarah O. , Subbotin Sergei. A.
Published: September 2019

In 2018 to 2019, soil and root samples from some declining peach orchards were collected in Edgefield County, South Carolina, USA. Excavated roots of Guardian® peach (Prunus persica) rootstock showed strong gall symptoms. Extracted root-knot nematodes (RKN) were identified by both morphological and molecular methods as M. floridensis. This is the first detection of the peach RKN in South Carolina and the third state in the USA after Florida and California.
First Report of Scutellonema brachyurus (Steiner, 1938) Andrassy, 1958 and Occurrence of Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 in Belgium
Authors: Nguyen Huu Tien , Trinh Quang Phap , Couvreur Marjolein , Singh Phougeishangbam Rolish , Decraemer Wilfrida , Bert Wim
Published: September 2019

A study of plant-parasitic nematodes in the Botanical garden at Ghent University in Belgium revealed the presence of two tropical nematode species, i.e. Scutellonema brachyurus and Meloidogyne incognita. Scutellonema brachyurus was recovered, only once, for the first time in Belgium from Musa basjoo and is morphologically characterized. M. incognita, forming galls on Hedychium greenii, was recovered in all seasons over three consecutive years and is morphologically and molecularly characterized. Although no unequivocal evidence was found to indicate that these nematodes pose a current threat in Belgium, in the light of climate change, it is crucial to improve our knowledge of potential tropical nematode activity in more Northern countries.
Logarithmic correlation functions for critical dense polymers on the cylinder
Authors: Morin-Duchesne Alexi , Jacobsen Jesper
Published: September 2019

We compute lattice correlation functions for the model of critical
dense polymers on a semi-infinite cylinder of perimeter
nn.
In the lattice loop model, contractible loops have a vanishing fugacity
whereas non-contractible loops have a fugacity
\alpha \in (0,\infty)α∈(0,∞).
These correlators are defined as ratios Z(x)/Z_0Z(x)/Z0
of partition functions, where Z_0Z0
is a reference partition function wherein only simple half-arcs are
attached to the boundary of the cylinder. For
Z(x)Z(x),
the boundary of the cylinder is also decorated with simple half-arcs,
but it also has two special positions 11
and xx
where the boundary condition is different. We investigate two such kinds
of boundary conditions: (i) there is a single node at each of these
points where a long arc is attached, and (ii) there are pairs of
adjacent nodes at these points where two long arcs are attached. We find
explicit expressions for these correlators for finite
nn
using the representation of the enlarged periodic Temperley-Lieb algebra
in the XX spin chain. The resulting asymptotics as
n\to \inftyn→∞
are expressed as simple integrals that depend on the scaling parameter
\tau = \frac {x-1} n \in (0,1)τ=x−1n∈(0,1).
For small \tauτ,
the leading behaviours are proportional to
\tau^{1/4}τ1/4,
\tau^{1/4}\log \tauτ1/4logτ,
\log \taulogτ
and \log^2 \taulog2τ.
We interpret the lattice results in terms of ratios of conformal
correlation functions. We assume that the corresponding boundary
changing fields are highest weight states in irreducible, Kac or
staggered Virasoro modules, with central charge
c=-2c=−2
and conformal dimensions \Delta = -\frac18Δ=−18
or \Delta = 0Δ=0.
With these assumptions, we obtain differential equations of order two
and three satisfied by the conformal correlation functions, solve these
equations in terms of hypergeometric functions, and find a perfect
agreement with the lattice results. We use the lattice results to
compute structure constants and ratios thereof which appear in the
operator product expansions of the boundary condition changing fields.
The fusion of these fields is found to be non-abelian.
Gain without pain: an international case for a tradable green certificates system to foster renewable energy development in Ukraine
Authors: Kurbatova Tetiana , Sidortsov Roman , Sotnyk Iryna , Telizhenko Oleksandr , Skibina Tetiana , Roubík Hynek
Published: September 2019

This paper elaborates on the theoretical and methodological fundamentals of a tradable green certificates system to foster renewable energy development in Ukraine. It proposes a management mechanism premised on the classical market model of tradable green certificates aiming at increasing the share of electricity from renewable energy sources in the country’s energy mix. Organizational stages of the mechanism formation at the national level and a methodological approach to assess green electricity generation cost are developed. The modeling has shown that the annual increase in the cap for green electricity consumption by 1% will raise the electricity tariff by 3%, which is not a significant financial burden for consumers. The proposed changes in the tradable green certificates system can be an effective management tool to achieve the required amount of electricity from renewable energy sources in the country’s total electricity consumption and to foster the development of the Ukrainian renewable energy sector.
Objetos de Aprendizaje y Recursos Educativos Abiertos en Educación Superior
Authors: Colomé Dunia
Published: September 2019

La tecnología Objeto de Aprendizaje fue una de las soluciones brindadas a la necesidad de tener recursos educativos reutilizables que contribuyeran a un mayor acceso al conocimiento. Los objetos de apredizaje, desde su concepción, aportan numerosas ventajas a la reutilización y a la accesibilidad de los contenidos, sin embargo en la práctica, su empleo no ha sido generalizado por diversas dificultades técnicas que presentan. La evolución de la definición de los objetos de aprendizaje junto a la necesidad creciente de producir recursos educativos bajo una perspectiva socialmente responsable, dio surgimiento a los recursos educativos abiertos. Estos recursos son importantes materiales, caracterizados por su potencial para facilitar la expansión del aprendizaje; en ocasiones se confunden con los objetos de aprendizaje, empleándose indistintamente ambos términos. En este trabajo se presenta una comparación entre los objetos de aprendizaje y los recursos educativos abiertos, destacando sus puntos de contactos y diferencias, así como su empleo en la Educación Superior.
Tutorización virtual de trabajos final de grado y máster: Valoración de la experiencia por parte del alumnado
Authors: Lizandra Jorge
Published: September 2019

Se presenta una investigación educativa en la que se describe un modelo de tutorización virtual de trabajos final de grado y máster. Se pretende conocer la utilidad y eficacia del método a partir de las valoraciones del alumnado.El estudio se enmarca en el paradigma de investigación cualitativa. Participaron 14 estudiantes, que realizaron un seguimiento del desarrollo de su trabajo, de cuya información se realizó un análisis de contenido.Los resultados indican que la tutorización virtual ha tenido una buena aceptación por parte del alumnado. Se destaca que facilita la organización del trabajo, así como la comunicación e interacción docente-discente. Por tanto, se considera que podría ser una práctica de interés para el profesorado de educación superior.
Estrategias socioafectivas factibles de aplicar en ambientes virtuales de aprendizaje
Authors: Morales Salas Rubí Estela , Curiel Peón Laura
Published: September 2019

Este artículo se relaciona con las estrategias socioafectivas factibles de aplicarse en ambientes virtuales de aprendizaje (AVA) para optimizar el proceso formativo y el desarrollo moral y profesional de estudiantes y profesores.

Mediante el método de análisis de contenido y un diagrama de Pareto, se identificaron algunas estrategias socioafectivas que, de acuerdo con la percepción de los participantes, son factibles de aplicarse en AVA.

Según resultados el 70 y 100 por ciento de los asesores, identificaron 11 estrategias de un total de 15, con alta importancia para aplicarlas en su proceso virtual de enseñanza aprendizaje.

 Las autoras consideran que esos resultados serán útiles en futuros estudios relacionados con las necesidades socioafectivas de los estudiantes que optan por modalidad virtual, así como para identificar necesidades de formación docente referidas a la educación socioafectiva.
Percepción docente conforme al uso de los recursos digitales en la pizarra digital interactiva con alumnos de Necesidades Educativas Especiales
Authors: Vico Linde Paula
Published: September 2019

Se presentan los resultados obtenidos en la investigación realizada para analizar cómo repercute el uso de la Pizarra Digital Interactiva en el proceso de enseñanza-aprendizaje con alumnos con Necesidades Educativas Especiales. El objetivo de este estudio es conocer la percepción de los docentes de Pedagogía Terapéutica y, Audición y Lenguaje, sobre el potencial de los recursos digitales cuando trabajan con la PDI y el uso que realizan de la misma. Además, de conocer el comportamiento de los alumnos cuando trabajan con este dispositivo. Se ha utilizado una metodología cualitativa con dos instrumentos para la recogida de datos: pautas de observación y cuestionario. Los resultados obtenidos demuestran que los docentes tienen una buena percepción de la PDI ya que favorece el desarrollo de aprendizajes funcionales, pero es necesario llevar a cabo un planteamiento metodológico favorable.
TÜRK TOPLUMUNDA SURİYELİLERE YÖNELİK ALGILANAN TEHDİTLER VE ÇÖZÜM ÖNERİLERİ
Authors: EKİCİ Hatice
Published: September 2019

Suriye
krizinin patlak vermesiyle birlikte, dünya genelinde başka bir ülkeye sığınma
talebinde bulunan mültecilerin sayısında büyük oranda artış meydana gelmiştir.
Takip ettiği açık kapı politikasıyla, Suriye krizinden en çok etkilenen
ülkelerin başında Türkiye gelmiş, ve en çok Suriyeli mülteciyi ağırlayan ülke
konumuna dönüşmüştür. Başlarda Türk toplumunun Suriyeli mültecilere karşı
tutumları misafirperverlik anlayışından yola çıkarak pozitifken, Suriyelilerin
sayılarının ve kalış sürelerinin artmasıyla birlikte, bu tutum yerini yabancı
düşmanlığı güden tutumlara bırakmıştır.  Alanyazında ki çalışmalar, göçmenlere ve
mültecilere yönelik negatif tutum ve davranışların altında yatan en önemli
faktörlerden birinin tehdit algısı olduğunu ortaya koymaktadır. Bu makalede,
Türk toplumunun Suriyeli mültecilere yönelik tehdit algıları ele alınmış olup,
bu tehdit algıları Bütünleşik Tehdit Kuramı, Sistemi Sürdürme Kuramı, Bileşik
Araçsal Grup Çatışma Modeli ve Hastalıktan Kaçınma Modeli çerçevesinde
açıklanmıştır. Türk toplumunun Suriyeli mültecileri 3 farklı alanda tehdit
olarak gördüğü sonucuna varılmıştır: (1) Kültürel ve toplumsal, (2) ekonomik,
(3) temel hizmet alanlarına erişim. Toplumdaki
tehdit algıları incelendikten sonra, bu tehdit algılarının sebep olabileceği
çatışma alanları ele alınmış, ve toplumsal uyum için çözüm önerileri
sunulmuştur.
SUÇA SÜRÜKLENEN ÇOCUKLARIN DEĞERLENDİRİLMESİNDE EKOLOJİK SİSTEM YAKLAŞIMININ KULLANIMI: OLGU SUNUMU
Authors: ZORBAZ Osman , BİLGE Filiz
Published: September 2019

Bu çalışmanın amacı, suça sürüklenen çocuklara yapılan psikososyal müdahalelerde ekolojik sistem yaklaşımının kullanılmasını bir olgu çerçevesinde incelemektir. Olgu; 16 yaşında, Ankara Çocuk Mahkemesine Şubat 2015 ayında “taksirle bir kişinin yaralanmasına neden olma” suçundan hakkında ceza davası açılan ve çalışmaya katılmayı kabul eden M’dir. M’ye öncelikle çalışma hakkında bilgi verilmiş ve hazırlanan yarı yapılandırılmış görüşme formu çerçevesinde görüşmeler yapılmıştır. Çalışmada olgunun psikososyal durumu ekolojik sistem yaklaşımı katmanlarına göre koruyucu ve risk faktörleri olarak açıklanmış ve bu doğrultuda yapılan müdahaleler izlenmiştir. Çalışma sonucunda ekolojik sistem yaklaşımının suça sürüklenen çocuklara yapılan müdahalelerde etkili biçimde kullanılabileceği değerlendirilmektedir.
An optimized bearing parameter identification approach from vibration response spectra
Authors: Mutra Rajasekhara Reddy , J Srinivas
Published: September 2019

In the present work, an effective identification methodology bearing dynamic parameters using measured vibration responses at the bearing is proposed. The flexible rotor is analyzed by using finite element beam model with nonlinear hydrodynamic bearing forces due to floating ring bearing supports. The frequency domain responses at different operating speeds are initially obtained in both the lateral directions. The error function is formulated as an average difference in amplitudes of two lateral displacements at a bearing node with known reference signals over a frequency range. The design variables are the speed dependent direct and cross-coupled stiffness and damping parameters of the bearing. With the side constraints on the variables, the error is minimized by using a modified particle swarm optimization scheme. The accuracy of the approach is tested with noisy input signals.
Method for the extraction of shock signal features based on the upper limit of density integral
Authors: Yang Haikun , Pan Hongxia
Published: September 2019

Shock signal features must be extracted for use in pattern recognition or fault diagnosis. In this work, we proposed a method for the feature extraction of shock signals, which are vibration signals that change faster and have larger amplitude ranges than general signals. First, we proposed the concepts of amplitude density for monotonic functions and piecewise monotonic functions. On the basis of these concepts, we then proposed the concept of the upper limit of density integral (ULDI), which was adopted to obtain signal features. Then, we introduced two types of serious fault cracks to the latch sheet of an automatic gun mechanism that is used on warships. Next, we applied the proposed method to extract the features of shock signals from data acquired when the automatic gun mechanism fired with normal and two fault patterns. Finally, we verified the effectiveness of our proposed method by applying the features that it extracted to a support vector machine (SVM). Our proposed method provided good results and was superior to the traditional statistics-based feature extraction method when applied to a SVM for classification. In addition, the former method demonstrated better generalisation than the latter. Thus, our method is an efficient approach for extracting shock signal features in pattern recognition and fault diagnosis.
Chaperones Promote Remarkable Solubilization of Salmonella enterica serovar Enteritidis Flagellin Expressed in Escherichia coli
Authors: Bakhtiarvand Bahador , Sadeghi Zahra , Tarahomjoo Shirin , Yaghmaie Soheila
Published: September 2019

Background:

Flagellin of Salmonella enterica serovar Enteritidis (SEF) stimulates
immune responses to both itself and coapplied antigens. It is therefore used in vaccine development
and immunotherapy. Removal of pathogenic S. enterica ser. Enteritidis from SEF production
process is advantageous due to the process safety improvement. The protein solubility analysis
using SDS-PAGE indicated that 53.49% of SEF expressed in Escherichia coli formed inclusion
bodies. However, the protein recovery from inclusion bodies requires a complex process with a low
yield.

Objective:

We thus aim to study possibility of enhancing SEF expression in E. coli in soluble form
using chemical and molecular chaperones.

Methods:

Chemical chaperones including arginine, sorbitol, trehalose, sodium chloride and benzyl
alcohol were used as cultivation medium additives during SEF expression. SEF solubilization by
coexpression of molecular chaperones DnaK, DnaJ, and GrpE was also investigated.

Results:

All of the chemical chaperones were effective in improving SEF solubility. However,
sorbitol showed the most profound effect. SEF solubilization by molecular chaperones was slightly
better than that using sorbitol and this approach enhanced noticeably SEF soluble concentration and
SEF solubility percentage to almost two folds and 96.37% respectively. Results of limited
proteolysis assay and native PAGE indicated similar conformational states and proper folding for
SEF obtained without using chaperones and for those obtained using sorbitol and the molecular
chaperones. However, the molecular chaperones based system was less costly than the sorbitol
based system.

Conclusion:

The coexpression of molecular chaperones was then considered as the most
appropriate approach for soluble SEF production. Therefore, SEF production for medical purposes
is expected to be facilitated.
MiR-144-3p Inhibits BMSC Proliferation and Osteogenic Differentiation Via Targeting FZD4 in Steroid-Associated Osteonecrosis
Authors: Sun Zhibo , Wu Fei , Yang Yue , Liu Feng , Mo Fengbo , Chen Jin , Wang Guangyong , Zhang Bo
Published: September 2019

Background:

MicroRNAs have recently been recognized to be engaged in the development of bone
diseases.

Objective:

This study was performed to elucidate the effects of miR-144-3p on proliferation and osteogenesis of
mesenchymal stem cells (MSCs) from the patients with steroid-associated osteonecrosis (ONFH) and its related
mechanism.

Method:

The expression level of miR-144-3p in the MSCs from the proximal femur of the patients was examined
by Real-time PCR. The cell proliferation ability was assayed by MTT. The differentiation ability of MSCs was
assayed by Alizarin Red S (ARS) staining. The interaction between miR-144-3p and frizzled4 (FZD4) was investigated
by Real-time PCR, western blot and luciferase reporter assay.

Results:

ONFH samples had the obviously high expression of miR-144-3p compared to the control. MiR-144-3p
had a negative effect on the proliferation and osteogenesis of MSCs. Via targeting FZD4, miR-144-3p decreased
β-catenin nuclear translocation, the transcription of RUNX2 and COL1A1. Over-expression of FZD4 partially
reversed miR-144-3p-induced decrease in the proliferation and osteogenesis of MSCs.

Conclusion:

MiR-144-3p might play an important role in the development of ONFH and might be used as a
novel class of therapeutic targets for this disease.
Anti-IgE Significantly Changes Circulating Interleukin-25, Vitamin-D and Interleukin-33 Levels in Patients with Allergic Asthma
Authors: Yalcin Arzu D. , Uzun Rusen
Published: September 2019

Background:

Multi-center, randomized-controlled trials and observational studies have demonstrated
that, in severe asthmatic patients receiving omalizumab treatment, the frequency of exacerbations, the number of
urgent adverse events, and the need for oral steroids tend to decrease.

Material and Method:

This study included a total of 32 patients. The patients were divided into two groups as
Group IA (pre-omalizumab) and Group IB (post-omalizumab). Serum IL-25 and IL-33 levels were measured and
the number of emergency admissions, length of hospitalization (day), Asthma Control Test (ACT) scores, eosinophil
cationic protein (ECP), and fractional exhaled nitric oxide (FeNO) value were analyzed.

Results:

ACT and FeNO values increased after omalizumab treatment, while IL-33, IL-25 levels decreased after
the completion of omalizumab treatment. Furthermore, there was a weak, positive, and significant relationship
between the changes in the ECP levels and IL-33 levels (r=0.38, p=0.03).

Conclusion:

To the best of our knowledge, this is the first study to compare circulating IL-25 and IL-33 levels
with specific IgE synthesis in the literature. Multivariate correlation analysis showed that the changes in serum
IL-33 levels were significantly correlated with the changes in the mite sIgE levels and length of hospital stay
(Fmodel=11.2, p=0.01, r2=0.45). On the other hand, there was no significant relationship between the other variables
and changes in the IL-25 levels.
RP-HPLC Estimation of Flavonol Derivatives and Phenolic Acids in Capsicum annuum L. and their Correlation with In Vitro Anti-Inflammatory Activity
Authors: Iqbal Zahra , Ahmad Ausaf , Haque Ziyaul , Khan Mohammed S. , Khan Mohd S. , Iqbal Mohammed S.
Published: September 2019

Background:

Flavonol derivative and phenolic acids derived from the plants function as free radical scavengers, reducing agents, and quenchers for the formation of singlet oxygen. Flavonoids and phenolic constituents also play an important role in various human diseases and disorders primarily through modulation of inflammatory responses.

Objective:

To estimate the Flavonol Derivatives (FD) and phenolic acids (PA) in Capsicum annuum (CA) and other important phytochemicals having an anti-inflammatory effect.

Method:

In the present study, FD and PA were estimated in CA and in vitro anti-inflammatory activity (pilot study) was determined and correlation was established.

Results:

The results were found to be significant using RP-HPLC. FD and PA were found to be 0.0659±0.0058 and 0.0862±0.0.0134 mg/gram dry weight, respectively. For in vitro anti-inflammatory activity, the inhibition of albumin denaturation and antiproteinase activity was found to be maximum in Quercetin (QE) with 98.230±1.589% and 59.906±1.529%, respectively. Heat-induced hemolysis of erythrocytes was found to be maximum in salicylic acid (SA) (71.830±2.838%). Hypotonicity-induced hemolysis showed significant activity with QE (76.770±3.475%). Lipoxygenase and cyclooxygenase inhibition was found to be maximum in QE with 56.930±4.069% and 61.660±3.135%, respectively.

Conclusion:

A strong positive correlation of 0.9 was observed between the extract of CA and standard QE and SA against the anti-inflammatory activity. Therefore, the role of FD and PA has been postulated to be an active phytochemical of CA accountable for its anti-inflammatory activity. However further work is desirable to fully elucidate the phytochemicals responsible for their anti-inflammatory activity and to develop better herbal drug formulations.
Synthesis,In VitroEvaluation, Molecular Docking and DFT Studies of Some Phenyl Isothiocyanates as Anticancer Agents
Authors: Richa Kikoleho , Karmaker Rituparna , Longkumer Naruti , Das Vishal , Bhuyan Pulak J. , Pal Mintu , Sinha Upasana B.
Published: September 2019

Background:

Isothiocyanates (ITCs) are small molecules that are important in synthetic organic chemistry, but their actual importance lies in their potential as anti-carcinogens. Through this piece of work, an effort was made to assess the anti-cancer activity of some simple ITCs which can be synthesized through easy greener pathways.

Methods:

Cell proliferation assay was performed on ovarian cancer cells (PA-1) and non-tumorigenic ovarian epithelial cells (IOSE-364). Furthermore, qRT-PCR for transcript expression levels of Spindlin1 and caspases in ovarian cancer cells and cell cycle analysis was performed. In silico studies were incorporated to understand the mode of ligand-protein interaction, ADME/Toxicity and drug-likeliness parameters. Density functional theory studies have been also been employed on the ITCs to assess their efficiency in anticancer activity.

Results:

An inexpensive, environmentally benign pathway has been developed for synthesizing a series of ITCs. Among the synthesized ITCs, NC6 showed better cytotoxic effects as compared to its counterparts. Novel findings revealed that NC6 had 5-folds lower transcript expression levels of Spindlin1 and induced caspases 3 and 7 expressions assessed by qRT-PCR in ovarian cancer cells. Furthermore, flow cytometry assay showed the cell cycle arrest at G1/S phase of cell cycle. The molecular docking studies revealed favorable binding affinities and the physiochemical parameters were predicted to be compatible with drug-likeliness.

Conclusion:

The results demonstrated the possibility that small isothiocyanate molecules which can be synthesized by a simple green methodology, can pose as promising candidates for their application as anticancer agents.
Synergistic Effect of α-Solanine and Cisplatin Induces Apoptosis and Enhances Cell Cycle Arrest in Human Hepatocellular Carcinoma Cells
Authors: El-Daly Sherien M. , Gouhar Shaimaa A. , Gamal-Eldeen Amira M. , Abdel Hamid Fatma F. , Ashour Magdi N. , Hassan Nahla S.
Published: September 2019

Aim:

The clinical application of cisplatin is limited by severe side effects associated with high applied doses. The synergistic effect of a combination treatment of a low dose of cisplatin with the natural alkaloid α-solanine on human hepatocellular carcinoma cells was evaluated.

Methods:

HepG2 cells were exposed to low doses of α-solanine and cisplatin, either independently or in combination. The efficiency of this treatment modality was evaluated by investigating cell growth inhibition, cell cycle arrest, and apoptosis enhancement.

Results:

α-solanine synergistically potentiated the effect of cisplatin on cell growth inhibition and significantly induced apoptosis. This synergistic effect was mediated by inducing cell cycle arrest at the G2/M phase, enhancing DNA fragmentation and increasing apoptosis through the activation of caspase 3/7 and/or elevating the expression of the death receptors DR4 and DR5. The induced apoptosis from this combination treatment was also mediated by reducing the expression of the anti-apoptotic mediators Bcl-2 and survivin, as well as by modulating the miR-21 expression.

Conclusion:

Our study provides strong evidence that a combination treatment of low doses of α-solanine and cisplatin exerts a synergistic anticancer effect and provides an effective treatment strategy against hepatocellular carcinoma.
Photographic record of Thraupis episcopus (Blue gray Tanager) in an urban environment on the central coast of Oaxaca, Mexico
Authors: García-Grajales Jesús , Buenrostro-Silva Alejandra
Published: September 2019

We present a photographic record of the Blue-gray Tanager (Thraupis episcopus) in an urban environment on the central coast of Oaxaca, Mexico. The nearest geographically known records are from the southeastern coastal plain of Guerrero, northeastern Mexico and the Yucatan Peninsula. In Oaxaca, the unpublished visual records are from the coastal plain of this state and can be seen on digital platforms; however, it is necessary to publish records in order to facilitate the analysis of passive dispersion of neotropical birds and document its possible causes.
DARI DAMARWULAN KE JINGGOAN: DINAMIKA KESENIAN JANGER DI BANYUWANGI 1930’AN-1970
Authors: Nurullita Hervina -
Published: September 2019

ABSTRAKPenelitian ini berangkat dari sebuah pertanyaan bagaimana sejarah kesenian Janger yang sangat populer di Banyuwangi berkembang dengan pesat dan masih menjadi minat masyarakat dari tahun 1930’an sampai sekarang. Janger yang merupakan akulturasi dari Jawa, Bali dan Banyuwangi terus dilestarikan oleh para seniman pendukungnya. Janger identik dengan lakon Damarwulan-Minakjinggo pun menjadi perdebatan antara sejarah dan fiksi. Penelitian ini menggunakan metode penelitian sejarah yang terdiri dari heuristik, kritik, interpretasi dan historiografi. Hasil penelitian menyebutkan bahwa akulturasi dari seni Janger inilah yang menyebabkan kelestarian Janger Banyuwangi. Selain itu banyak dari seniman Banyuwangi yang menggantungkan hidupnya dari kesenian ini. Kata Kunci: Janger, Banyuwangi, Damarwulan-Minakjinggo, Akulturasi. ABSTRACTThis research come from the question history of Janger which too popular in Banyuwangi and still exist since 1930st until now. Janger is tradisional drama which acculturation from Java, Bali and Bali. Janger identic with story about Damarwulan-Minakjinggo. This story stil argued fiction or non fiction. Thi research using historical method such as heuristic, critic, interpretation and historiography. Result from this research know that acculturation between Java, Bali and Banyuwangi which make them sustainable until now. And the other many artist depend on their life in this drama.  Keywords: Janger, Banyuwangi, Damarwulan-Minakjinggo, Acculturation
Characteristics, Opportunities, and Challenges of Osteopathy (COCO) in the Perceptions of Osteopaths in Germany, Austria, and Switzerland: Protocol for a Comprehensive Mixed Methods Study
Authors: Porthun Jan , Manschel Jonas
Published: September 2019

Background

Currently, the importance of osteopathy within the health care system is controversial. The training structures and the acknowledgment of the occupational profile strongly differ in the German-speaking territory.

Objective

This study aims to examine the characteristics of the osteopathic profession as well as the possibilities and challenges for osteopaths in Germany, Austria, and Switzerland.

Methods

This study adopted a mixed methods design. The research topic will be examined based on qualitative and quantitative partial studies that will be conducted in parallel as well as sequentially. By applying different research methods and sample testing and by using standardized, validated measurement methods, we expect to be able to gain new insights into the work area of osteopathy.

Results

In November 2018, we started the research and data collection. Currently, we are conducting the first two partial studies. The planned duration of each of the partial study is 6-9 months. The project is scheduled to be completed in 2021.

Conclusions

This study will examine how osteopaths define themselves in comparison with professionals from other occupational profiles and how they describe the characteristics of their work. The identification of central issues is expected to help clarify the issues and define the profession. As such, the results might contribute to the conservation and improvement of the quality of osteopathic treatment.

International Registered Report Identifier (IRRID)

PRR1-10.2196/15399
A Biofeedback App to Instruct Abdominal Breathing (Breathing-Mentor): Pilot Experiment (Preprint)
Authors: Faust-Christmann Corinna Anna , Taetz Bertram , Zolynski Gregor , Zimmermann Tobias , Bleser Gabriele
Published: September 2019

BACKGROUND

Deep and slow abdominal breathing is an important skill for the management of stress and pain. However, despite multiple proofs on the effectiveness of biofeedback, most breathing apps remain limited to pacing specific breathing patterns, without sensor feedback on the actual breathing behavior.

OBJECTIVE

To fill this gap, an app named Breathing-Mentor was developed. This app combines effective visualization of the instruction with biofeedback on deep abdominal breathing, based on the mobile phone’s accelerometers. The aim of this pilot study was to investigate users’ feedback and breathing behavior during initial contact with the app.

METHODS

To reveal the possible effects of biofeedback, two versions of the mobile app were developed. Both contained the same visual instruction, but only the full version included additional biofeedback. In total, 40 untrained participants were randomly assigned to one of the two versions of the app. They had to follow the app’s instructions as closely as possible for 5 min.

RESULTS

The group with additional biofeedback showed an increased signal-to-noise ratio for instructed breathing frequency (0.1 Hz) compared with those using visual instruction without biofeedback (F1,37=4.18; P<.048). During this initial contact with the full version, self-reported relaxation effectivity was, however, lower than the group using visual instruction without biofeedback (t37=−2.36; P=.02), probably owing to increased cognitive workload to follow the instruction.

CONCLUSIONS

This study supports the feasibility and usefulness of incorporating biofeedback in the Breathing-Mentor app to train abdominal breathing. Immediate effects on relaxation levels should, however, not be expected for untrained users.