bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficient Finite Element Modeling of Steel Cables in Reinforced Rubber
Authors: Pletz Martin , Frankl Siegfried Martin , Schuecker Clara
Published: May 2022

Spiral steel cables feature complex deformation behavior due to their wound geometry. In applications where the cables are used to reinforce rubber components, modeling the cables is not trivial, because the cable’s outer surface must be connected to the surrounding rubber material. There are several options for modeling steel cables using beam and/or solid elements for the cable. So far, no study that lists and evaluates the performance of such approaches can be found in the literature. This work investigates such modeling options for a simple seven-wire strand that is regarded as a cable. The setup, parameter calibration, and implementation of the approaches are described. The accuracy of the obtained deformation behavior is assessed for a three-cable specimen using a reference model that features the full geometry of the wires in the three cables. It is shown that a beam approach with anisotropic beam material gives the most accurate stiffness results. The results of the three-cable specimen model indicate that such a complex cable model is quite relevant for the specimen’s deformation. However, there is no single approach that is well suited for all applications. The beam with anisotropic material behavior is well suited if the necessary simplifications in modeling the cable–rubber interface can be accepted. The present work thus provides a guide not only for calibrating but also for selecting the cable-modeling approach. It is shown how such modeling approaches can be used in commercial FE software for applications such as conveyor belts.
Computational Contact Pressure Prediction of CoCrMo, SS 316L and Ti6Al4V Femoral Head against UHMWPE Acetabular Cup under Gait Cycle
Authors: Jamari J. , Ammarullah Muhammad Imam , Santoso Gatot , Sugiharto S. , Supriyono Toto , Prakoso Akbar Teguh , Basri Hasan , van der Heide Emile
Published: May 2022

Due to various concerns about the use of metal-on-metal that is detrimental to users, the use of metal as acetabular cup material was later changed to ultra high molecular weight polyethylene (UHMWPE). However, the wear on UHMWPE releases polyethylene wear particles, which can trigger a negative body response and contribute to osteolysis. For reducing the wear of polyethylene, one of the efforts is to investigate the selection of metal materials. Cobalt chromium molybdenum (CoCrMo), stainless steel 316L (SS 316L), and titanium alloy (Ti6Al4V) are the frequently employed materials. The computational evaluation of contact pressure was carried out using a two-dimensional axisymmetric model for UHMWPE acetabular cup paired with metal femoral head under gait cycle in this study. The results show Ti6Al4V-on-UHMWPE is able to reduce cumulative contact pressure compared to SS 316L-on-UHMWPE and CoCrMo-on-UHMWPE. Compared to Ti6Al4V-on-UHMWPE at peak loading, the difference in cumulative contact pressure to respective maximum contact pressure is 9.740% for SS 316L-on-UHMWPE and 11.038% for CoCrMo-on-UHMWPE.
Damage Estimation of a Concrete Pier When Exposed to Extreme Flood and Debris Loading
Authors: Nasim Maryam , Setunge Sujeeva
Published: May 2022

The structural safety and serviceability during extreme weather, such as floods and storms, is critical. Due to global warming in the last decades, the increase in the intensity of natural disasters, i.e., flood loading and the durability of the road structures and infrastructures, is becoming critical. Bridges and structures lose their capacity because of ageing over time. On the other hand, the load intensity is another reason for the structural damage. Debris loading due to the flooding on bridges is one of the reasons for the increase in flood loading and eventually structural damage. Measuring the level of structural damage under extreme events is vital in determining the vulnerability and resilience of structures during a disaster. A damage index (DI) can be defined as a measurement tool for the levels of structural damage. Oftentimes, damage indices are developed to measure the deterioration of the system under earthquake loading. Little work has been published on damage indices (DIs) under flood loading, where a uniform pressure is applied to a structure. This paper presents a comprehensive review of DIs published in the literature and compares two approaches to assess the system’s damage utilising finite element methodologies. The structure model developed in the ABAQUS software package is used to predict the failure of a concrete component under applied lateral loading. The model is validated using published experimental work. The model is verified, and then it is used to compute the damage indicators using two primary techniques, including a deflection-based method and an energy loss-based approach. Using the two offered DIs, the change in damage levels is displayed underwater flow uniform loading. A comparison of the two methods is conducted. In this paper, differences between the two concepts are analysed and presented.
Exposure to Essential and Toxic Elements via Consumption of Agaricaceae, Amanitaceae, Boletaceae, and Russulaceae Mushrooms from Southern Spain and Northern Morocco
Authors: Barea-Sepúlveda Marta , Espada-Bellido Estrella , Ferreiro-González Marta , Bouziane Hassan , López-Castillo José Gerardo , Palma Miguel , F. Barbero Gerardo
Published: May 2022

The demand and interest in mushrooms, both cultivated and wild, has increased among consumers in recent years due to a better understanding of the benefits of this food. However, the ability of wild edible mushrooms to accumulate essential and toxic elements is well documented. In this study, a total of eight metallic elements and metalloids (chromium (Cr), arsenic (As), cadmium (Cd), mercury (Hg), lead (Pb), copper (Cu), zinc (Zn), and selenium (Se)) were determined by ICP-MS in five wild edible mushroom species (Agaricus silvicola, Amanita caesarea, Boletus aereus, Boletus edulis, and Russula cyanoxantha) collected in southern Spain and northern Morocco. Overall, Zn was found to be the predominant element among the studied species, followed by Cu and Se. The multivariate analysis suggested that considerable differences exist in the uptake of the essential and toxic elements determined, linked to species-intrinsic factors. Furthermore, the highest Estimated Daily Intake of Metals (EDIM) values obtained were observed for Zn. The Health Risk Index (HRI) assessment for all the mushroom species studied showed a Hg-related cause of concern due to the frequent consumption of around 300 g of fresh mushrooms per day during the mushrooming season.
Respiratory Epithelial Cells: More Than Just a Physical Barrier to Fungal Infections
Authors: Barros Bianca C. S. C. , Almeida Bruna R. , Barros Debora T. L. , Toledo Marcos S. , Suzuki Erika
Published: May 2022

The respiratory epithelium is highly complex, and its composition varies along the conducting airways and alveoli. In addition to their primary function in maintaining the respiratory barrier and lung homeostasis for gas exchange, epithelial cells interact with inhaled pathogens, which can manipulate cell signaling pathways, promoting adhesion to these cells or hosting tissue invasion. Moreover, pathogens (or their products) can induce the secretion of chemokines and cytokines by epithelial cells, and in this way, these host cells communicate with the immune system, modulating host defenses and inflammatory outcomes. This review will focus on the response of respiratory epithelial cells to two human fungal pathogens that cause systemic mycoses: Aspergillus and Paracoccidioides. Some of the host epithelial cell receptors and signaling pathways, in addition to fungal adhesins or other molecules that are responsible for fungal adhesion, invasion, or induction of cytokine secretion will be addressed in this review.
Childhood Sporting Experience and Charitable Donations to Disaster Victims
Authors: Yamamura Eiji
Published: May 2022

I investigated how people’s childhood experiences of involvement in team sports helped them develop non-cognitive skills, which later prompted them to make charitable donations to disaster victims. I independently collected individual-level data from approximately 7000 observations in 2016. The instrumental variable (IV) method was used for the estimations. In the specification of the IV model, sporting experience and informal education in childhood were used as exogenous IV. I found that (1) sporting experiences cause people to have positive subjective views of reciprocity, (2) team sports experience has a larger effect on people than individual sports experience, and (3) the above lead people to donate to disaster victims of enormous disasters such as the Great East Japan Earthquake.
Extraction and Pronoun Preposing in Scandinavian
Authors: Engdahl Elisabet , Lindahl Filippa
Published: May 2022

It has been noted that examples with extractions out of relative clauses that have been attested in Danish, Norwegian and Swedish are judged to be unacceptable in Icelandic and Faroese. We hypothesize that this may reflect whether or not speakers tend to prepose unstressed object pronouns as a way of establishing a coherent discourse. In this article we investigate to what extent pronoun preposing is used in Swedish, Icelandic and Faroese and whether there is any correlation with the acceptabilty of extractions from relative clauses. We show that Icelandic speakers use pronoun preposing to a very limited extent whereas Faroese speakers often prepose the VP or sentential anaphor tað. In both languages extraction from relative clauses is mainly judged to be unacceptable, with Faroese speakers being somewhat more accepting of extraction from presentational relatives. A crucial factor seems to be whether preposing is associated with a marked, contrastive interpretation or not.
Fabrication and Sensing Application of Phase Shifted Bragg Grating Sensors
Authors: Sun Xiaoyan , Zeng Li , Hu Youwang , Duan Ji’an
Published: May 2022

As a special kind of Bragg grating, phase-shifted fiber Bragg grating (PS-FBG) has attracted extensive attention because of its extremely narrow transmission window and excellent sensing performance. The main purpose of this manuscript is to discuss the PS-FBG with special sensing characteristics and explore the influence of different inscription technologies on the sensing characteristics of PS-FBG by comparing the existing inscription methods. The sensing characteristics, advantages and disadvantages of PS-FBG with different structures are analyzed.
Electrochemical Behavior of SiC-Coated AA2014 Alloy through Plasma Electrolytic Oxidation
Authors: Aljohani Talal A. , Alawad Majed O. , Elkatatny Sally , Alateyah Abdulrahman I. , Rubayan Meteb T. Bin , Alhajji Mohammed A. , AlBeladi Muntathir I. , Khoshnaw Fuad , El-Garaihy Waleed H.
Published: May 2022

In this study, the corrosion performance of AA2014 aluminum alloy was enhanced by coating the alloy with a layer containing silica (SiC) that was formed by the plasma electrolytic oxidation (PEO) process. The PEO process was performed with different electrical parameters (frequency, current mode, and duty ratio) and both with and without SiC to investigate the microstructural and electrochemical differences in the coated samples produced from the process. The microstructure and composition of the PEO coatings were studied using X-ray diffraction (XRD) and scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS). A potentiodynamic polarization test and electrochemical impedance spectroscopy (EIS) were used to investigate the electrochemical behavior of the AA2014-PEO-coated samples. The potentiodynamic polarization showed that the SiC-PEO-coated samples had a significantly decreased corrosion rate (99.8%) compared with the uncoated AA2014 Al alloy. Our results showed that the coats containing SiC possessed a much higher corrosion resistance than both the uncoated AA2014 Al alloy (8,344,673%) and the SiC-free coatings, which possess low corrosion resistance, because of their higher chemical stability and more compact microstructure.
Potential of Rapid Tooling in Rapid Heat Cycle Molding: A Review
Authors: Huzaim Nurul Hidayah Mohamad , Rahim Shayfull Zamree Abd , Musa Luqman , Abdellah Abdellah El-hadj , Abdullah Mohd Mustafa Al Bakri , Rennie Allan , Rahman Rozyanti , Garus Sebastian , Błoch Katarzyna , Sandu Andrei Victor , Vizureanu Petrica , Nabiałek Marcin
Published: May 2022

Rapid tooling (RT) and additive manufacturing (AM) are currently being used in several parts of industry, particularly in the development of new products. The demand for timely deliveries of low-cost products in a variety of geometrical patterns is continuing to increase year by year. Increased demand for low-cost materials and tooling, including RT, is driving the demand for plastic and rubber products, along with engineering and product manufacturers. The development of AM and RT technologies has led to significant improvements in the technologies, especially in testing performance for newly developed products prior to the fabrication of hard tooling and low-volume production. On the other hand, the rapid heating cycle molding (RHCM) injection method can be implemented to overcome product surface defects generated by conventional injection molding (CIM), since the surface gloss of the parts is significantly improved, and surface marks such as flow marks and weld marks are eliminated. The most important RHCM technique is rapid heating and cooling of the cavity surface, which somewhat improves part quality while also maximizing production efficiencies. RT is not just about making molds quickly; it also improves molding productivity. Therefore, as RT can also be used to produce products with low-volume production, there is a good potential to explore RHCM in RT. This paper reviews the implementation of RHCM in the molding industry, which has been well established and undergone improvement on the basis of different heating technologies. Lastly, this review also introduces future research opportunities regarding the potential of RT in the RHCM technique.
Computational Design and Characterisation of Gyroid Structures with Different Gradient Functions for Porosity Adjustment
Authors: Wallat Leonie , Altschuh Patrick , Reder Martin , Nestler Britta , Poehler Frank
Published: May 2022

Triply periodic minimal surface (TPMS) structures have a very good lightweight potential, due to their surface-to-volume ratio, and thus are contents of various applications and research areas, such as tissue engineering, crash structures, or heat exchangers. While TPMS structures with a uniform porosity or a linear gradient have been considered in the literature, this paper focuses on the investigation of the mechanical properties of gyroid structures with non-linear porosity gradients. For the realisation of the different porosity gradients, an algorithm is introduced that allows the porosity to be adjusted by definable functions. A parametric study is performed on the resulting gyroid structures by performing mechanical simulations in the linear deformation regime. The transformation into dimensionless parameters enables material-independent statements, which is possible due to linearity. Thus, the effective elastic behaviour depends only on the structure geometry. As a result, by introducing non-linear gradient functions and varying the density of the structure over the entire volume, specific strengths can be generated in certain areas of interest. A computational design of porosity enables an accelerated application-specific structure development in the field of engineering.
Polarization Independent Metamaterial Absorber with Anti-Reflection Coating Nanoarchitectonics for Visible and Infrared Window Applications
Authors: Musa Ahmad , Hakim Mohammad Lutful , Alam Touhidul , Islam Mohammad Tariqul , Alshammari Ahmed S. , Mat Kamarulzaman , M. M. Salaheldeen , Almalki Sami H. A. , Islam Md. Shabiul
Published: May 2022

The visible and infrared wavelengths are the most frequently used electromagnetic (EM) waves in the frequency spectrum; able to penetrate the atmosphere and reach Earth’s surface. These wavelengths have attracted much attention in solar energy harvesting; thermography; and infrared imaging applications for the detection of electrical failures; faults; or thermal leakage hot spots and inspection of tapped live energized components. This paper presents a numerical analysis of a compact cubic cross-shaped four-layer metamaterial absorber (MA) structure by using a simple metal-dielectric-metal-dielectric configuration for wideband visible and infrared applications. The proposed MA achieved above 80% absorption in both visible and near-infrared regions of the spectrum from 350 to 1250 nm wavelength with an overall unit cell size of 0.57λ × 0.57λ × 0.59λ. The SiO2 based anti-reflection coating of sandwiched tungsten facilitates to achieve the wide high absorption bandwidth. The perceptible novelty of the proposed metamaterial is to achieve an average absorptivity of 95.3% for both visible and infrared wavelengths with a maximum absorptivity of 98% from 400 nm to 900 nm. Furthermore, the proposed structure provides polarization insensitivity with a higher oblique incidence angle tolerance up to 45°.
Silk Vascular Grafts with Optimized Mechanical Properties for the Repair and Regeneration of Small Caliber Blood Vessels
Authors: Valsecchi Elisa , Biagiotti Marco , Alessandrino Antonio , Gastaldi Dario , Vena Pasquale , Freddi Giuliano
Published: May 2022

As the incidence of cardiovascular diseases has been growing in recent years, the need for small-diameter vascular grafts is increasing. Considering the limited success of synthetic grafts, vascular tissue engineering/repair/regeneration aim to find novel solutions. Silk fibroin (SF) has been widely investigated for the development of vascular grafts, due to its good biocompatibility, tailorable biodegradability, excellent mechanical properties, and minimal inflammatory reactions. In this study, a new generation of three-layered SF vascular scaffolds has been produced and optimized. Four designs of the SILKGraft vascular prosthesis have been developed with the aim of improving kink resistance and mechanical strength, without compromising the compliance with native vessels and the proven biocompatibility. A more compact arrangement of the textile layer allowed for the increase in the mechanical properties along the longitudinal and circumferential directions and the improvement of the compliance value, which approached that reported for the saphenous and umbilical veins. The higher braid density slightly affected the grafts’ morphology, increasing surface roughness, but the novel design mimicked the corrugation approach used for synthetic grafts, causing significant improvements in kink resistance.
Electrochemical Corrosion Behavior of MIG-Welded 7N01-T4 Aluminum Alloy by ER5356 and ER5087 Welding Wires
Authors: Wei Ping , Wu Mingfang , Liu Dashuang , Zhao Ziqiang , Liang Yun , Dong Zhihui
Published: May 2022

7N01-T4 aluminum alloy plates were welded by the metal inert gas (MIG) welding method, with ER5087 and ER5356 welding wires, respectively. The electrochemical corrosion behavior of the weld zones in the two kinds of welded joints using 3.5 wt.% and 5 wt.% NaCl solutions were investigated by polarization curve, electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM), and laser confocal scanning microscope (LCSM). The results indicated the better corrosion resistance of the weld zone in the ER5356 welded joint than that in the ER5087 welded joint, which was related to the different contents of Mn and Zn elements and the distribution of precipitates for the weld zones in the two kinds of welded joints. Based on the LSCM of the weld zones, the maximum depth (dmax) of corrosion pits for the weld zone in the ER5356 welded joint was lower than that in the ER5087 welded joint when immersed in the same NaCl concentrations. The dmax of the corrosion pit of the weld zone in the ER5356 welded joint using the 5 wt.% NaCl solution was 78.5 ± 0.96 μm, which was much bigger than that using the 3.5 wt.% NaCl solution. For the weld zone in the ER5087 welded joint with 5 wt.% NaCl solution, more Cl- was adsorbed onto the active surface of weld zones, which accelerated the corrosion, resulting in the corrosion mechanism from pitting to intergranular corrosion.
A Wind Turbine Fault Classification Model Using Broad Learning System Optimized by Improved Pelican Optimization Algorithm
Authors: Tuerxun Wumaier , Xu Chang , Haderbieke Muhaxi , Guo Lei , Cheng Zhiming
Published: May 2022

As a classification model, a broad learning system is widely used in wind turbine fault diagnosis. However, the setting of hyperparameters for the models directly affects the classification accuracy of the models and it generally relies on practical experience and prior knowledge. In order to effectively solve the problem, the parameters of the broad learning system such as the number of feature nodes, the number of enhancement nodes, and the number of mapped features layer were optimized by the improved pelican optimization algorithm, and a classification model was built based on the broad learning system optimized by the improved pelican optimization algorithm. The classification accuracy of the proposed model was the highest and reached 98.75%. It is further shown that compared with the support vector machine, deep belief networks, and broad learning system models optimized by particle swarm optimization algorithm, the proposed model effectively improves the accuracy of wind turbine fault diagnosing.
Prediction of the Comprehensive Error Field in the Machining Space of the Five-Axis Machine Tool Based on the “S”-Shaped Specimen Family
Authors: Wu Shi , Dong Zeyu , Qi Fei , Fan Zhendong
Published: May 2022

In order to quickly and accurately predict the spatial geometric error field of the five-axis machine tool processing, a method for predicting the comprehensive error field of the five-axis machine tool processing space based on the “S”-shaped specimen family is studied. Firstly, for the five-axis CNC machine tool in the form of A-C dual turntable, the geometric error model of the rotating axis is established based on the multi-body dynamics theory; the error mapping relationship between the processing technology system and the workpiece is analyzed based on the “S”-shaped specimen family, and the identification of 12 geometric errors of the two rotating shafts. Then, the error value of the sampling point is measured based on the “S”-shaped test piece in machine contact, and the double-circle center coordinate value is determined according to the curvature of the measured wire of the test piece, in order to identify the geometric errors of the two rotation axes of the five-axis machine tool. Finally, based on the prediction method, the comprehensive error field of the five-axis CNC machine tool processing space is analyzed. Compared with other geometric error identification methods, the measurement accuracy of this method meets the processing requirements and can further evaluate the comprehensive performance of the machine tool.
Stability and Numerical Simulations of a New SVIR Model with Two Delays on COVID-19 Booster Vaccination
Authors: Liu Xinyu , Ding Yuting
Published: May 2022

As COVID-19 continues to threaten public health around the world, research on specific vaccines has been underway. In this paper, we establish an SVIR model on booster vaccination with two time delays. The time delays represent the time of booster vaccination and the time of booster vaccine invalidation, respectively. Second, we investigate the impact of delay on the stability of non-negative equilibria for the model by considering the duration of the vaccine, and the system undergoes Hopf bifurcation when the duration of the vaccine passes through some critical values. We obtain the normal form of Hopf bifurcation by applying the multiple time scales method. Then, we study the model with two delays and show the conditions under which the nontrivial equilibria are locally asymptotically stable. Finally, through analysis of official data, we select two groups of parameters to simulate the actual epidemic situation of countries with low vaccination rates and countries with high vaccination rates. On this basis, we select the third group of parameters to simulate the ideal situation in which the epidemic can be well controlled. Through comparative analysis of the numerical simulations, we concluded that the most appropriate time for vaccination is to vaccinate with the booster shot 6 months after the basic vaccine. The priority for countries with low vaccination rates is to increase vaccination rates; otherwise, outbreaks will continue. Countries with high vaccination rates need to develop more effective vaccines while maintaining their coverage rates. When the vaccine lasts longer and the failure rate is lower, the epidemic can be well controlled within 20 years.
Bayesian Networks for Preprocessing Water Management Data
Authors: Ropero Rosa Fernández , Flores María Julia , Rumí Rafael
Published: May 2022

Environmental data often present inconveniences that make modeling tasks difficult. During the phase of data collection, two problems were found: (i) a block of five months of data was unavailable, and (ii) no information was collected from the coastal area, which made flood-risk estimation difficult. Thus, our aim is to explore and provide possible solutions to both issues. To avoid removing a variable (or those missing months), the proposed solution is a BN-based regression model using fixed probabilistic graphical structures to impute the missing variable as accurately as possible. For the second problem, the lack of information, an unsupervised classification method based on BN was developed to predict flood risk in the coastal area. Results showed that the proposed regression solution could predict the behavior of the continuous missing variable, avoiding the initial drawback of rejecting it. Moreover, the unsupervised classifier could classify all observations into a set of groups according to upstream river behavior and rainfall information, and return the probability of belonging to each group, providing appropriate predictions about the risk of flood in the coastal area.
Application of HMM and Ensemble Learning in Intelligent Tunneling
Authors: Pan Yongbo , Zhu Xunlin
Published: May 2022

The cutterhead torque and thrust, reflecting the obstruction degree of the geological environment and the behavior of excavation, are the key operating parameters for the tunneling of tunnel boring machines (TBMs). In this paper, a hybrid hidden Markov model (HMM) combined with ensemble learning is proposed to predict the value intervals of the cutterhead torque and thrust based on the historical tunneling data. First, the target variables are encoded into discrete states by means of HMM. Then, ensemble learning models including AdaBoost, random forest (RF), and extreme random tree (ERT) are employed to predict the discrete states. On this basis, the performances of those models are compared under different forms of the same input parameters. Moreover, to further validate the effectiveness and superiority of the proposed method, two excavation datasets including Beijing and Zhengzhou from the actual project under different geological conditions are utilized for comparison. The results show that the ERT outperforms the other models and the corresponding prediction accuracies are up to 0.93 and 0.99 for the cutterhead torque and thrust, respectively. Therefore, the ERT combined with HMM can be used as a valuable prediction tool for predicting the cutterhead torque and thrust, which is of positive significance to alert the operator to judge whether the excavation is normal and assist the intelligent tunneling.
Advanced Control Algorithm for FADEC Systems in the Next Generation of Turbofan Engines to Minimize Emission Levels
Authors: Aghasharifian Esfahani Majid , Namazi Mohammadmehdi , Nikolaidis Theoklis , Jafari Soheil
Published: May 2022

New propulsion systems in aircrafts must meet strict regulations and emission limitations. The Flightpath 2050 goals set by the Advisory Council for Aviation Research and Innovation in Europe (ACARE) include reductions of 75%, 90%, and 65% in CO2, NOx, and noise, respectively. These goals are not fully satisfied by marginal improvements in gas turbine technology or aircraft design. A novel control design procedure for the next generation of turbofan engines is proposed in this paper to improve Full Authority Digital Engine Control (FADEC) systems and reduce the emission levels to meet the Flightpath 2050 regulations. Hence, an Adaptive Network–based Fuzzy Inference System (ANFIS), nonlinear autoregressive network with exogenous inputs (NARX) techniques, and the block-structure Hammerstein–Wiener approach are used to develop a model for a turbofan engine. The Min–Max control structure is chosen as the most widely used practical control algorithm for gas turbine aero engines. The objective function is considered to minimize the emission level for the engine in a pre-defined maneuver while keeping the engine performance in different aspects. The Genetic Algorithm (GA) is applied to find the optimized control structure. The results confirm the effectiveness of the proposed approach in emission reduction for the next generation of turbofan engines.
On Robustness for Spatio-Temporal Data
Authors: García-Pérez Alfonso
Published: May 2022

The spatio-temporal variogram is an important factor in spatio-temporal prediction through kriging, especially in fields such as environmental sustainability or climate change, where spatio-temporal data analysis is based on this concept. However, the traditional spatio-temporal variogram estimator, which is commonly employed for these purposes, is extremely sensitive to outliers. We approach this problem in two ways in the paper. First, new robust spatio-temporal variogram estimators are introduced, which are defined as M-estimators of an original data transformation. Second, we compare the classical estimate against a robust one, identifying spatio-temporal outliers in this way. To accomplish this, we use a multivariate scale-contaminated normal model to produce reliable approximations for the sample distribution of these new estimators. In addition, we define and study a new class of M-estimators in this paper, including real-world applications, in order to determine whether there are any significant differences in the spatio-temporal variogram between two temporal lags and, if so, whether we can reduce the number of lags considered in the spatio-temporal analysis.
Cutaneous Ulcer Caused by Apixaban Treatment Is Resolved after Replacement with Dabigatran
Authors: Medoro Alessandro , Passarella Daniela , Mignogna Donatella , Porcile Carola , Foderà Emanuele , Intrieri Mariano , Raimo Gennaro , La Floresta Pancrazio , Russo Claudio , Martucci Gennaro
Published: May 2022

Nowadays, novel oral anticoagulants (NOACs) have shown improved safety profile and efficacy compared to vitamin K antagonists in the prevention of thromboembolic events occurring during different pathological conditions. However, there are concerns and safety issues, mostly related to adverse events following interactions with other drugs, in real-world practice. We report the case of an 83-year-old woman who developed a non-bleeding leg ulcer not caused by trauma or other evident pathological conditions after 10 days of treatment with apixaban 5 mg/q.d. She was switched from apixaban to dabigatran and the leg ulcer rapidly improved and completely cicatrized in 40 days. The resolution of the ulcer and the toleration of dabigatran therapy suggest an apixaban-specific reaction; however, the pathological mechanism of ulcer onset is currently unclear. Careful evaluation of hospital databases of Molise region (Southern Italy) hospitals identified two similar cases between 2019 and 2021. These cases underline the necessity of careful post-marketing surveillance, considering the rapidly increasing number of patients treated with NOACs and patient’s risk factors such as old age, high polypharmacy rate, co-morbidities, and peculiar genetic background related to NOACs pharmacokinetic features.
Approach to Thrombophilia in Pregnancy—A Narrative Review
Authors: Samfireag Miruna , Potre Cristina , Potre Ovidiu , Tudor Raluca , Hoinoiu Teodora , Anghel Andrei
Published: May 2022

Thrombophilia is a genetic predisposition to hypercoagulable states caused by acquired haemostasis conditions; pregnancy causes the haemostatic system to become hypercoagulable, which grows throughout the pregnancy and peaks around delivery. Genetic testing for thrombophilic gene mutations is evaluated using different methodologies of real-time polymerase chain reaction and DNA microarrays of specific genes. Adapting the general care of the pregnant woman to the particularities caused by thrombophilia is an important component, so screening is preferred to assess the degree of genetic damage that manifests itself as a risk of thrombosis. The major goal of this narrative review was to quantitatively evaluate the literature data on the specific care of pregnant women with thrombophilia that are at risk of developing unplanned miscarriages.
Clinical Impact of Immune Checkpoint Inhibitor (ICI) Response, DNA Damage Repair (DDR) Gene Mutations and Immune-Cell Infiltration in Metastatic Melanoma Subtypes
Authors: Andrieu Charlotte , McNamee Niamh , Larkin Anne-Marie , Maguire Alanna , Menon Roopika , Mueller-Eisert Judith , Horgan Noel , Kennedy Susan , Gullo Giuseppe , Crown John , Walsh Naomi
Published: May 2022

Molecular and histopathological analysis of melanoma subtypes has revealed distinct epidemiological, genetic, and clinical features. However, immunotherapy for advanced metastatic melanoma patients does not differ based on subtype. Response to immune checkpoint inhibitors (ICI) has been shown to vary, therefore, predictive biomarkers are needed in the design of precision treatments. Targeted sequencing and histopathological analysis (CD8 and CD20 immunohistochemistry) were performed on subtypes of metastatic melanoma (cutaneous melanoma (CM, n = 10); head and neck melanoma (HNM, n = 7); uveal melanoma (UM, n = 4); acral lentiginous melanoma (AM, n = 1) and mucosal melanoma (MM, n = 1) treated with ICI). Progression-free survival (PFS) was significantly associated with high CD8 expression (p = 0.025) and mutations in DNA damage repair (DDR) pathway genes (p = 0.012) in all subtypes but not with CD20 expression. Our study identified that immune cell infiltration and DDR gene mutations may have an impact in response to ICI treatment in metastatic melanoma but differs among subtypes. Therefore, a comprehensive understanding of the immune infiltration cells’ role and DDR gene mutations in metastatic melanoma may identify prognostic biomarkers.
A 66–76 GHz Wide Dynamic Range GaAs Transceiver for Channel Emulator Application
Authors: Zhou Peigen , Wang Chen , Sun Jin , Chen Zhe , Chen Jixin , Hong Wei
Published: May 2022

In this study, we developed a single-channel channel emulator module with an operating frequency covering 66–67 GHz, including a 66–76 GHz wide dynamic range monolithic integrated circuit designed based on 0.1 µm pHEMT GaAs process, a printed circuit board (PCB) power supply bias network, and low-loss ridge microstrip line to WR12 (60–90 GHz) waveguide transition structure. Benefiting from the on-chip multistage band-pass filter integrated at the local oscillator (LO) and radio frequency (RF) ends, the module’s spurious components at the RF port were greatly suppressed, making the module’s output power dynamic range over 50 dB. Due to the frequency-selective filter integrated in the LO chain, each clutter suppression in the LO chain exceeds 40 dBc. Up and down conversion loss of the module is better than 14 dB over the 66–67 GHz band, the measured IF input P1 dB is better than 10 dBm, and the module consumes 129 mA from a 5 V low dropout supply. A low-loss ridged waveguide ladder transition was designed (less than 0.4 dB) so that the output interface of the module is a WR12 waveguide interface, which is convenient for direct connection with an instrument with E-band (60–90 GHz) waveguide interface.
Infrared Transmission Characteristics of Phase Transitioning VO2 on Various Substrates
Authors: Azad Samee , Gajula Durga , Sapkota Nawraj , Rao Apparao , Koley Goutam
Published: May 2022

Infrared transmission characteristics of VO2 thin films synthesized on multiple substrates, using a low-pressure direct oxidation technique, have been characterized. Material characterization of these films indicates high material quality, which resulted in large variation of electrical and optical properties at phase transition. A change in optical transmissivity greater than 80% was observed for these films utilizing infrared (IR) laser illumination at 1550 nm. Phase transition enabled by temperature change induced by a pulsed high-power laser beam resulted in modulated IR laser transmission with a low time constant in VO2 on transparent quartz and muscovite substrates. Investigation of the effect of mechanical strain on phase transition in VO2 grown on flexible muscovite substrate indicate shift in transition temperature to higher for tensile and lower for compressive strains.
Fabrication of Irregularity-Free, Highly Circular Cross-Sectional Microchannel
Authors: Inagaki Satoru , Ishida Tadashi
Published: May 2022

Microchannels typically have rectangular cross-sections, whereas biological channels, such as blood vessels and airways, have circular cross-sections. The difference in cross-sections causes different fluidic behaviors, resulting in differences between fluidic behavior in microchannels and biological channels. To simulate fluidic behavior in vitro, circular cross-sectional microchannels are required. We developed a circular cross-sectional microchannel with a smooth channel wall, free from cracks and irregularities. In the fabrication process of the circular cross-sectional microchannel, uncured polydimethylsiloxane (PDMS) was inserted into a rectangular cross-sectional microchannel. Then, the PDMS was pushed out by the introduction of compressed air before the curing process. During the introduction of compressed air, we observed the behavior of the uncured PDMS and found the formation and movement of protrusions along the microchannel. After pushing out the uncured PDMS, the residual PDMS was cured, resulting in a circular cross-sectional microchannel. The fabrication method was examined by varying the channel orientation and airflow direction. In the case of the horizontal orientation and airflow in an opposite direction, the circularity of the microchannel was greater than 0.99 at the centimeter scale in all cross-sections along the microchannel.
Whole-Genome Sequencing Reveals Multiple Subpopulations of Dominant and Persistent Lineage I Isolates of Listeria monocytogenes in Two Meat Processing Facilities during 2011–2015
Authors: Burnett Elton , Kucerova Zuzana , Freeman Molly , Kathariou Sophia , Chen Jessica , Smikle Monica
Published: May 2022

Listeria monocytogenes is a foodborne pathogen with a highly clonal population structure comprising multiple phylogenetic sub-groups that can persist within food processing environments and contaminate food. The epidemiology of L. monocytogenes is well-described in some developed countries; however, little is known about the prevalence and population structure of this pathogen in food and food processing environments located in less developed regions. The aim of this study was to determine the genetic characteristics and clonal relatedness of L. monocytogenes that were isolated from two Jamaican meat processing facilities. Of the 37 isolates collected between 2011 and 2015, only a single lineage II isolate was recovered (serotype 1/2c), and the remaining were lineage I isolates representing serotypes 4b, 1/2b, 3b, and two untypeable isolates. Pulsed-field gel electrophoresis (PFGE) delineated isolates into seven pulsotypes, and whole-genome sequencing (WGS) categorized most isolates within one of three clonal complexes (CC): CC2 (N = 12), CC5 (N = 11), and CC288 (N = 11). Isolates representing CC1 (N = 2) and CC9 (N = 1) were also recovered. Virulence-associated genes such as inlA and the LIPI-3 cluster were detected in multiple isolates, along with the stress survival islet cluster-1 (SSI-1), and benzalkonium (bcrABC) and cadmium (cad1, cad2, cad4) resistance cassettes. Multiple isolates that belong to the same CC and matching PFGE patterns were isolated from food and the environment from both facilities across multiple years, suggesting the presence of persistent strains of L. monocytogenes, and/or constant re-entry of the pathogens into the facilities from common sources. These findings highlight the ability of lineage I isolates of L. monocytogenes to colonize, persist, and predominate within two meat-producing environments, and underscores the need for robust surveillance strategies to monitor and mitigate against these important foodborne pathogens.
Isolation and Properties of the Bacterial Strain Janthinobacterium sp. SLB01
Authors: Chernogor Lubov , Bakhvalova Kseniya , Belikova Alina , Belikov Sergei
Published: May 2022

Bacteria of the genus Janthinobacterium are widespread in soils and freshwater ecosystems and belong to the phylum Proteobacteria. The Janthinobacterium sp. SLB01 strain was isolated from diseased freshwater Lubomirskia baicalensis (Pallas, 1776) sponge, and the draft genome was published previously. However, the properties of the SLB01 strain are not known. The aim of the study is to describe some properties of the Janthinobacterium sp. SLB01 strain, isolated from L. baicalensis sponge. The identification of the SLB01 strain was established as Gram-negative, motile, rod-shaped, and psychrotolerant, with growth at 3 and 22 °C. We found that the SLB01 strain has proteolytic, lipolytic, and saccharolytic activity and can use citrates and reduce nitrates. The bacteria Janthinobacterium sp. SLB01 strain can grow, form biofilms, and produce the violet pigment violacein. We identified the pigments violacein and deoxyviolacein by chromatography and mass spectrometry. These metabolites may be of interest to biotechnology in the future. The studied characteristics of the Janthinobacterium sp. SLB01 strain are an important addition to previous studies of the genome of this strain. This study will help us to understand the relationship between the microbial communities of Lake Baikal and sponges.
Safety Assessment and Pain Relief Properties of Saffron from Taliouine Region (Morocco)
Authors: Ait Tastift Maroua , Makbal Rachida , Bourhim Thouria , Omari Zineb , Isoda Hiroko , Gadhi Chemseddoha
Published: May 2022

Saffron is the most expensive spice in the world. In addition to its culinary utilization, this spice is used for medicinal purposes such as in pain management. In this study, the analgesic activity of Crocus sativus stigma extract (CSSE) was evaluated in rodents and its possible physiological mechanism was elucidated. The anti-nociceptive effect of CSSE was evaluated using three animal models (hot plate, writhing, and formalin tests). The analgesic pathways involved were assessed using various analgesia-mediating receptors antagonists. The oral administration of CSSE, up to 2000 mg/kg, caused no death or changes in the behavior or in the hematological and biochemical blood parameters of treated animals nor in the histological architecture of the animals’ livers and kidneys. CSSE showed a central, dose-dependent, anti-nociceptive effect in response to thermal stimuli; and a peripheral analgesic effect in the test of contortions induced by acetic acid. The dual (central and peripheral) analgesic effect was confirmed by the formalin test. The anti-nociceptive activity of CSSE was totally or partially reversed by the co-administration of receptor antagonists, naloxone, atropine, haloperidol, yohimbine, and glibenclamide. CSSE influenced signal processing, by the modulation of the opioidergic, adrenergic, and muscarinic systems at the peripheral and central levels; and by regulation of the dopaminergic system and control of the opening of the ATP-sensitive K+ channels at the spinal level. The obtained data point to a multimodal mechanism of action for CSSE: An anti-inflammatory effect and a modulation, through different physiological pathways, of the electrical signal generated by the nociceptors. Further clinical trials are required to endorse the potential utilization of Moroccan saffron as a natural painkiller.
Fabrication and Evaluation of Voriconazole Loaded Transethosomal Gel for Enhanced Antifungal and Antileishmanial Activity
Authors: Farooq Mudassir , Usman Faisal , Zaib Sumera , Shah Hamid Saeed , Jamil Qazi Adnan , Akbar Sheikh Fatima , Khan Ajmal , Rabea Sameh , Hagras Soheir A. A. , El-Saber Batiha Gaber , Khan Imtiaz
Published: May 2022

Voriconazole (VRC) is a broad-spectrum antifungal agent belonging to BCS class II (biopharmaceutical classification system). Despite many efforts to enhance its solubility, this primary issue still remains challenging for formulation scientists. Transethosomes (TELs) are one of the potential innovative nano-carriers for improving the solubility and permeation of poorly soluble and permeable drugs. We herein report voriconazole-loaded transethosomes (VRCT) fabricated by the cold method and followed by their incorporation into carbopol 940 as a gel. The prepared VRCT were evaluated for % yield, % entrapment efficiency (EE), surface morphology, possible chemical interaction, particle size, zeta potential, and polydispersity index (PDI). The optimized formulation had a particle size of 228.2 nm, a zeta potential of −26.5 mV, and a PDI of 0.45 with enhanced % EE. Rheology, spreadability, extrudability, in vitro release, skin permeation, molecular docking, antifungal, and antileishmanial activity were also assessed for VRCT and VRC loaded transethosomal gel (VTEG). Ex-vivo permeation using rat skin depicted a transdermal flux of 22.8 µg/cm2/h with enhanced efficiency up to 4-fold. A two-fold reduction in inhibitory as well as fungicidal concentration was observed against various fungal strains by VRCT and VTEG besides similar results against L-donovani. The development of transethosomal formulation can serve as an efficient drug delivery system through a topical route with enhanced efficacy and better patient compliance.
The Selection of the Optimal Impregnation Conditions of Vegetable Matrices with Iodine
Authors: Zaremba Agata , Waszkowiak Katarzyna , Kmiecik Dominik , Jędrusek-Golińska Anna , Jarzębski Maciej , Szymandera-Buszka Krystyna
Published: May 2022

This study aimed to determine the use of selected vegetables (pumpkin, cauliflower, broccoli, carrot) as carriers of potassium iodide (KI) and potassium iodate (KIO3) by determining changes in iodine content under various conditions of impregnation as the degree of hydration, impregnated sample temperature, and impregnation time. The influence of these conditions on iodine contents in vegetables after their fortification and storage (21 °C/230 days) was analyzed. The results showed that all selected vegetables could be efficient iodine carriers. However, the conditions of the impregnation process are crucial for fortification efficiency, particularly the degree of hydration and the temperature of the impregnated samples before drying. The results showed that the lowest iodine content was in samples fortified at 4 °C and 1:4 hydration. On the other hand, the highest reproducibility of iodine was for the following fortification conditions: temperature of −76 °C and hydration of 1:1. The studies confirmed the higher stability of iodine in KIO3 form compared to KI. To increase recovery of the introduced iodine in the product after drying, using the conditioning step at 4 °C is not recommended. We recommend freezing vegetables immediately after the impregnation process