bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibition of (Pro)renin Receptor-Mediated Oxidative Stress Alleviates Doxorubicin-Induced Heart Failure
Authors: Du Xiao-yi , Xiang Dao-chun , Gao Ping , Peng Hua , Liu Ya-li
Published: April 2022

Aim

Clinical utility of doxorubicin (DOX) is limited by its cardiotoxic side effect, and the underlying mechanism still needs to be fully elucidated. This research aimed to examine the role of (pro)renin receptor (PRR) in DOX-induced heart failure (HF) and its underlying mechanism.

Main Methods

Sprague Dawley (SD) rats were injected with an accumulative dosage of DOX (15 mg/kg) to induce HF. Cardiac functions were detected by transthoracic echocardiography examination. The levels of lactate dehydrogenase (LDH) and creatine kinase (CK) in serum were detected, and oxidative stress related injuries were evaluated. Furthermore, the mRNA expression of PRR gene and its related genes were detected by real-time PCR (RT-PCR), and protein levels of PRR, RAC1, NOX4 and NOX2 were determined by Western blot. Reactive oxygen species (ROS) were determined in DOX-treated rats or cells. Additionally, PRR and RAC1 were silenced with their respective siRNAs to validate the in vitro impacts of PRR/RAC1 on DOX-induced cardiotoxicity. Moreover, inhibitors of PRR and RAC1 were used to validate their effects in vivo.

Key Findings

PRR and RAC1 expressions increased in DOX-induced HF. The levels of CK and LDH as well as oxidative stress indicators increased significantly after DOX treatment. Oxidative injury and apoptosis of cardiomyocytes were attenuated both in vivo and in vitro upon suppression of PRR or RAC1. Furthermore, the inhibition of PRR could significantly down-regulate the expressions of RAC1 and NOX4 but not that of NOX2, while the inhibition of RAC1 did not affect PRR.

Significance

Our findings showed that PRR inhibition could weaken RAC1-NOX4 pathway and alleviate DOX-induced HF via decreasing ROS production, thereby suggesting a promising target for the treatment of DOX-induced HF.
Advances of Patient-Derived Organoids in Personalized Radiotherapy
Authors: Wang Yuenan , Li Ye , Sheng Zonghai , Deng Weiwei , Yuan Hongyan , Wang Shubin , Liu Yajie
Published: April 2022

Patient-derived organoids (PDO), based on the advanced three-dimensional (3D) culture technology, can provide more relevant physiological and pathological cancer models, which is especially beneficial for developing and optimizing cancer therapeutic strategies. Radiotherapy (RT) is a cornerstone of curative and palliative cancer treatment, which can be performed alone or integrated with surgery, chemotherapy, immunotherapy, or targeted therapy in clinical care. Among all cancer therapies, RT has great local control, safety and effectiveness, and is also cost-effective per life-year gained for patients. It has been reported that combing RT with chemotherapy or immunotherapy or radiosensitizer drugs may enhance treatment efficacy at faster rates and lower cost. However, very few FDA-approved combinations of RT with drugs or radiosensitizers exist due to the lack of accurate and relevant preclinical models. Meanwhile, radiation dose escalation may increase treatment efficacy and induce more toxicity of normal tissue as well, which has been studied by conducting various clinical trials, very expensive and time-consuming, often burdensome on patients and sometimes with controversial results. The surged PDO technology may help with the preclinical test of RT combination and radiation dose escalation to promote precision radiation oncology, where PDO can recapitulate individual patient’ tumor heterogeneity, retain characteristics of the original tumor, and predict treatment response. This review aims to introduce recent advances in the PDO technology and personalized radiotherapy, highlight the strengths and weaknesses of the PDO cancer models, and finally examine the existing RT-related PDO trials or applications to harness personalized and precision radiotherapy.
The Role of Breastfeeding on Respiratory Outcomes Later in Childhood
Authors: Di Filippo Paola , Lizzi Mauro , Raso Massimiliano , Di Pillo Sabrina , Chiarelli Francesco , Attanasi Marina
Published: April 2022

Background

Breastfeeding is associated with a lower risk of wheezing in early childhood, but its effect later in childhood remains unclear. We investigated the association of breastfeeding and respiratory outcomes in children aged 11 years.

Materials and Methods

We performed an observational longitudinal study including 110 prepubertal children. Information about breastfeeding duration, wheezing and asthma was collected by questionnaires. At 11 years of age, we measured spirometry parameters, lung volumes, diffusing lung capacity, and fractional exhaled nitric oxide. We used logistic and linear regression models to examine the associations of breastfeeding duration with the odds of asthma and lung function measures. All multivariable analyses were adjusted for sex, smoking during pregnancy, gestational age at birth, twins, and mode of delivery (confounder model).

Results

Breastfeeding duration was associated with FEV1 z-score [β = 0.04, CI 95% (0.02–0.09)], FEF75 z-score [β = 0.06, CI 95% (0.03–0.09)] and FEV1/FVC z-score [β = 0.03, CI 95% (0.00–0.07)], but not with diffusing lung capacity and fractional exhaled nitric oxide. No association of breastfeeding duration with preschool wheezing, ever asthma and current asthma was documented.

Conclusion

We showed that children breastfed for longer time presented higher FEV1, FEV1/FVC, and FEF75 z-score values at 11 years of age compared to children breastfed for shorter time, suggesting a protective effect of breastfeeding on airways, and not on lung parenchyma (lung volumes and alveolar capillary membrane) or allergic airway inflammation. The positive effect of breastfeeding duration on lung function lays the foundation to promote breastfeeding more and more as effective preventive measure.
Coronavirus Disease 2019 Vaccination During Pregnancy and Breastfeeding: A Review of Evidence and Current Recommendations in Europe, North America, and Australasia
Authors: Pietrasanta Carlo , Ronchi Andrea , Crippa Beatrice Letizia , Artieri Giacomo , Ballerini Claudia , Crimi Riccardo , Mosca Fabio , Pugni Lorenza
Published: April 2022

In the late 2020s, less than 1 year into the coronavirus disease 2019 (COVID-19) pandemic, several anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines were introduced on a worldwide scale, with a significant positive impact on the consequences of the disease for several high-risk population groups. In the case of most bacterial or viral respiratory infections, pregnant women are at increased risk of complications, however, neither pregnant nor breastfeeding women were included in the first round of randomized clinical trials evaluating the safety and effectiveness of COVID-19 vaccines, because of safety and ethical concerns. Nevertheless, most anti-SARS-CoV-2 vaccines have not been expressly contraindicated during pregnancy or breastfeeding, and observational data on immune response, adverse effects, and clinical efficacy in pregnant and breastfeeding women have been progressively gathered during 2021. The vast majority of these data is reassuring for what concerns side effects for women and infants and points out the efficacy of vaccines in protecting women against COVID-19-related complications. Despite this, the hesitancy of pregnant and breastfeeding women at being vaccinated is still real. In this mini-review, we resume the available data on the clinical consequences of COVID-19 in pregnant women, as well as adverse effects, systemic and mucosal immune response, and clinical effectiveness of COVID-19 vaccines in pregnant and breastfeeding women. Moreover, we offer an updated overview of European, North American, and Australasian recommendations concerning COVID-19 vaccination in pregnant and breastfeeding women, in order to safely ensure the highest protection of women and their infants.
Kampo Formula-Pattern Models: The Development of 13 New Clinically Useful Standard Abdominal Pattern Models in the Fukushin Simulator
Authors: Yakubo Shuji , Baba Masaki , Odaguchi Hiroshi , Wakasugi Akino , Sekine Mariko , Hanawa Toshihiko , Mitsuma Tadamichi , Namiki Takao , Arai Makoto , Muramatsu Shin-Ichi , Shimada Yutaka , Shibahara Naotoshi
Published: April 2022

Aim: In Kampo medicine, there exists an important system of diagnosis called Fukushin, or abdominal diagnosis or palpation. By applying pressure to the abdomen of the patient, the physician can gain important information on the patient’s physical state and use those indications to choose a suitable Kampo formulation. We have previously developed a Fukushin simulator, a teaching tool that reproduces the important abdominal patterns that doctors will encounter in clinical practice and that has received favourable feedback for students and practitioners. In order to make diagnosis and prescription easier, it is desirable to have matched formula–pattern pairings. The present study aims to develop such pairings.

Methods: With the previously developed models as a foundation, in the present study the production team (two members) used materials such as urethane foam and silicone rubber to build an additional 13 standard abdominal pattern models matched to Kampo herbal formulas commonly used by practitioners in Japan. Subsequently, the evaluation team (the remaining 10 authors) investigated the viability of these models.

Results: The evaluation team determined that abdominal pattern models matched to the following typical Kampo formulas were created successfully: Dai-saiko-To (大柴胡湯), Dai-joki-To (大承気湯), Shigyaku-San (四逆散), Saiko-ka-ryukotsu-borei-To (柴胡加竜骨牡蛎湯), Keishi-bukuryo-Gan (桂枝茯苓丸), Hachimi-jio-Gan (八味地黄丸), Hange-shashin-To (半夏瀉心湯), Sho-saiko-To (小柴胡湯), Hochu-ekki-To (補中益気湯), Sho-kenchu-To (小建中湯), Toki-shakuyaku-San (当帰芍薬散), Ninjin-To (人参湯), and Dai-kenchu-To (大建中湯).

Conclusion: We suggest that these new formula-pattern models can make an important contribution to the standardization of abdominal diagnosis and prescription and to Kampo education.
Role of Nrf2 Signaling Cascade in Breast Cancer: Strategies and Treatment
Authors: Kumar Hitesh , Kumar Rachna M. , Bhattacharjee Devanjali , Somanna Preethi , Jain Vikas
Published: April 2022

Breast cancer is the second leading cancer among all types of cancers. It accounts for 12% of the total cases of cancers. The complex and heterogeneous nature of breast cancer makes it difficult to treat in advanced stages. The expression of various enzymes and proteins is regulated by several molecular pathways. Oxidative stress plays a vital role in cellular events that are generally regulated by nuclear factor erythroid 2-related factor 2 (Nrf2). The exact mechanism of Nrf2 behind cytoprotective and antioxidative properties is still under investigation. In healthy cells, Nrf2 expression is lower, which maintains antioxidative stress; however, cancerous cells overexpress Nrf2, which is associated with various phenomena, such as the development of drug resistance, angiogenesis, development of cancer stem cells, and metastasis. Aberrant Nrf2 expression diminishes the toxicity and potency of therapeutic anticancer drugs and provides cytoprotection to cancerous cells. In this article, we have discussed the attributes associated with Nrf2 in the development of drug resistance, angiogenesis, cancer stem cell generation, and metastasis in the specific context of breast cancer. We also discussed the therapeutic strategies employed against breast cancer exploiting Nrf2 signaling cascades.
Inventorization and Consensus Analysis of Ethnoveterinary Medicinal Knowledge Among the Local People in Eastern India: Perception, Cultural Significance, and Resilience
Authors: Mandal Suman Kalyan , Rahaman Chowdhury Habibur
Published: April 2022

Livestock is the main backbone of the rural economy of an agriculture-based country like India. To mitigate the economic loss due to livestock’s poor performance and illness, folk phytotherapy for livestock healthcare is still actively practiced in India. Literature survey revealed that the laterite region of eastern India, characterized by its cultural, ethnic, and biological diversities, as well as topographical uniqueness, lacks comprehensive information on ethnoveterinary medicinal knowledge. The objective of the present study includes documentation of traditional knowledge of ethnoveterinary medicine (EVM) from the northern laterite region in eastern India. Ethnoveterinary medicinal data were collected using a semi-structured questionnaire, free listing, and focus group discussions. The factor for informants’ consensus (Fic), fidelity level (FL), and cultural value (CV) index have been employed for quantitative analyses. Jaccard index (JI) was used to check the knowledge similarity. Altogether, 1,234 citations were made by 132 participants. In total, 232 recorded ethnomedicinal species are used for preparing 306 remedies to treat 79 health disorders of livestock. Recorded species are distributed in 92 families, and Fabaceae is identified as the most medicinally diversified. Uses of 24 angiospermic taxa, one pteridophyte, and two fungal species were exclusively new to the existing inventory of Indian traditional ethnoveterinary medicine. In 20 disease categories, the informant consensus (Fic) value ranges from 0.4 to 0.83. According to the FL value and use-mention factor, 23 EVM plants have been identified as the most important species in the respective disease categories. Value of CV index highlighted nine species as culturally most significant (CV ≥ 0.0025 and frequency of citation ≥20) in the laterite region of eastern India. A large extent of recorded data are quite worthy for the Indian folk veterinary medicinal repository. A handful of new data reported here and statistically justified culturally most significant species will provide the golden opportunity for bioprospecting research.
A 4/8 Subtype α-Conotoxin Vt1.27 Inhibits N-Type Calcium Channels With Potent Anti-Allodynic Effect
Authors: Wang Shuo , Bartels Peter , Zhao Cong , Yousuf Arsalan , Liu Zhuguo , Yu Shuo , Bony Anuja R. , Ma Xiaoli , Dai Qin , Sun Ting , Liu Na , Yang Mengke , Yu Rilei , Du Weihong , Adams David J. , Dai Qiuyun
Published: April 2022

A novel 4/8 subtype α-conotoxin, Vt1.27 (NCCMFHTCPIDYSRFNC-NH2), was identified from Conus vitulinus in the South China Sea by RACE methods. The peptide was synthesized and structurally characterized. Similar to other α-conotoxins that target neuronal nicotinic acetylcholine receptor (nAChR) subtypes, Vt1.27 inhibited the rat α3β2 nAChR subtype (IC50 = 1160 nM) and was inactive at voltage-gated sodium and potassium channels in rat sensory neurons. However, Vt1.27 inhibited high voltage-activated N-type (CaV2.2) calcium channels expressed in HEK293T cells with an IC50 of 398 nM. An alanine scan of the peptide showed that residues Phe5, Pro9, Ile10, and Ser13 contribute significantly to the inhibitory activity of Vt1.27. The molecular dockings indicate that Vt1.27 inhibits the transmembrane region of CaV2.2, which is different from that of ω-conotoxins. Furthermore, Vt1.27 exhibited potent anti-allodynic effect in rat partial sciatic nerve injury (PNL) and chronic constriction injury (CCI) pain models at 10 nmol/kg level with the intramuscular injection. The pain threshold elevation of Vt1.27 groups was higher than that of α-conotoxin Vc1.1 in CCI rat models. These findings expand our knowledge of targets of α-conotoxins and potentially provide a potent, anti-allodynic peptide for the treatment of neuropathic pain.
Numerical Simulation of Surface Dielectric Barrier Discharge With Functionally Graded Material
Authors: Zhang Zelin
Published: April 2022

Atmospheric-pressure surface dielectric barrier discharge (SDBD) has drawn significant attention, and the influence on the SDBD characteristics of surface dielectric barrier materials has been widely studied. In this work, a two-dimensional self-consistent fluid model is built to investigate the effect of barrier material characteristics on SDBD by introducing a linear permittivity distribution. It is demonstrated that a dielectric barrier with graded permittivity can affect the SDBD by changing the electric field distribution near the surface and speeding up the propagation of the streamer. The graded permittivity along the layer of the dielectric barrier plays a significant role in improving the dynamic characteristics of the SDBD.
Mechanisms of Isothiocyanate Detoxification in Larvae of Two Belowground Herbivores, Delia radicum and D. floralis (Diptera: Anthomyiidae)
Authors: Sontowski Rebekka , Guyomar Cervin , Poeschl Yvonne , Weinhold Alexander , van Dam Nicole M. , Vassão Daniel G.
Published: April 2022

Like aboveground herbivores, belowground herbivores are confronted with multiple plant defense mechanisms including complex chemical cocktails in plant tissue. Roots and shoots of Brassicaceae plants contain the two-component glucosinolate (GSL)-myrosinase defense system. Upon cell damage, for example by herbivore feeding, toxic and pungent isothiocyanates (ITCs) can be formed. Several aboveground-feeding herbivores have developed biochemical adaptation strategies to overcome the GSL-ITC defenses of their host plant. Whether belowground herbivores feeding on Brassica roots possess similar mechanisms has received little attention. Here, we analyze how two related belowground specialist herbivores detoxify the GSL-ITC defenses of their host plants. The larvae of the fly species Delia radicum and D. floralis are common pests and specialized herbivores on the roots of Brassicaceae. We used chemical analyses (HPLC-MS/MS and HPLC-UV) to examine how the GSL-ITC defense system is metabolized by these congeneric larvae. In addition, we screened for candidate genes involved in the detoxification process using RNAseq and qPCR. The chemical analyses yielded glutathione conjugates and amines. This indicates that both species detoxify ITCs using potentially the general mercapturic acid pathway, which is also found in aboveground herbivores, and an ITC-specific hydrolytic pathway previously characterized in microbes. Performance assays confirmed that ITCs negatively affect the survival of both species, in spite of their known specialization to ITC-producing plants and tissues, whereas ITC breakdown products are less toxic. Interestingly, the RNAseq analyses showed that the two congeneric species activate different sets of genes upon ITC exposure, which was supported by qPCR data. Based on our findings, we conclude that these specialist larvae use combinations of general and compound-specific detoxification mechanisms with differing efficacies and substrate preferences. This indicates that combining detoxification mechanisms can be an evolutionarily successful strategy to handle plant defenses in herbivores.
Metformin Alleviates Airway Hyperresponsiveness in a Mouse Model of Diet-Induced Obesity
Authors: Gu Chenjuan , Loube Jeff , Lee Rachel , Bevans-Fonti Shannon , Wu Tianshi David , Barmine Jessica H. , Jun Jonathan C. , McCormack Meredith C. , Hansel Nadia N. , Mitzner Wayne , Polotsky Vsevolod Y.
Published: April 2022

Obese asthma is a unique phenotype of asthma characterized by non-allergic airway hyperresponsiveness (AHR) and inflammation which responds poorly to standard asthma therapy. Metformin is an oral hypoglycemic drug with insulin-sensitizing and anti-inflammatory properties. The objective of the current study was to test the effect of metformin on AHR in a mouse model of diet-induced obesity (DIO). We fed 12-week-old C57BL/6J DIO mice with a high fat diet for 8 weeks and treated them with either placebo (control, n = 10) or metformin (n = 10) added in drinking water (300 mg/kg/day) during the last 2 weeks of the experiment. We assessed AHR, metabolic profiles, and inflammatory markers after treatments. Metformin did not affect body weight or fasting blood glucose, but significantly reduced serum insulin (p = 0.0117). Metformin reduced AHR at 30 mg/ml of methacholine challenge (p = 0.0052) without affecting baseline airway resistance. Metformin did not affect circulating white blood cell counts or lung cytokine mRNA expression, but modestly decreased circulating platelet count. We conclude that metformin alleviated AHR in DIO mice. This finding suggests metformin has the potential to become an adjuvant pharmacological therapy in obese asthma.
Image-Based Experimental Measurement Techniques to Characterize Velocity Fields in Blood Microflows
Authors: Le Andy Vinh , Fenech Marianne
Published: April 2022

Predicting blood microflow in both simple and complex geometries is challenging because of the composition and behavior of the blood at microscale. However, characterization of the velocity in microchannels is the key for gaining insights into cellular interactions at the microscale, mechanisms of diseases, and efficacy of therapeutic solutions. Image-based measurement techniques are a subset of methods for measuring the local flow velocity that typically utilize tracer particles for flow visualization. In the most basic form, a high-speed camera and microscope setup are the only requirements for data acquisition; however, the development of image processing algorithms and equipment has made current image-based techniques more sophisticated. This mini review aims to provide a succinct and accessible overview of image-based experimental measurement techniques to characterize the velocity field of blood microflow. The following techniques are introduced: cell tracking velocimetry, kymographs, micro-particle velocimetry, and dual-slit photometry as entry techniques for measuring various velocity fields either in vivo or in vitro.
Effects of Provenance, Growing Site, and Growth on Quercus robur Wood Anatomy and Density in a 12-Year-Old Provenance Trial
Authors: Hietz Peter , Rungwattana Kanin , Scheffknecht Susanne , George Jan-Peter
Published: April 2022

Vessels are responsible for an efficient and safe water transport in angiosperm xylem. Whereas large vessels efficiently conduct the bulk of water, small vessels might be important under drought stress or after winter when large vessels are embolized. Wood anatomy can adjust to the environment by plastic adaptation, but is also modified by genetic selection, which can be driven by climate or other factors. To distinguish between plastic and genetic components on wood anatomy, we used a Quercus robur trial where trees from ten Central European provenances were planted in three locations in Austria along a rainfall gradient. Because wood anatomy also adjusts to tree size and in ring-porous species, the vessel size depends on the amount of latewood and thereby ring width, we included tree size and ring width in the analysis. We found that the trees’ provenance had a significant effect on average vessel area (VA), theoretical specific hydraulic conductivity (Ks), and the vessel fraction (VF), but correlations with annual rainfall of provenances were at best weak. The trial site had a strong effect on growth (ring width, RW), which increased from the driest to the wettest site and wood density (WD), which increased from wet to dry sites. Significant site x provenance interactions were seen only for WD. Surprisingly, the drier site had higher VA, higher VF, and higher Ks. This, however, is mainly a result of greater RW and thus a greater proportion of latewood in the wetter forest. The average size of vessels > 70 μm diameter increased with rainfall. We argue that Ks, which is measured per cross-sectional area, is not an ideal parameter to compare the capacity of ring-porous trees to supply leaves with water. Small vessels (<70 μm) on average contributed only 1.4% to Ks, and we found no evidence that their number or size was adaptive to aridity. RW and tree size had strong effect on all vessel parameters, likely via the greater proportion of latewood in wide rings. This should be accounted for when searching for wood anatomical adaptations to the environment.
Optimizing Crop Water Use for Drought and Climate Change Adaptation Requires a Multi-Scale Approach
Authors: Burridge James D. , Grondin Alexandre , Vadez Vincent
Published: April 2022

Selection criteria that co-optimize water use efficiency and yield are needed to promote plant productivity in increasingly challenging and variable drought scenarios, particularly dryland cereals in the semi-arid tropics. Optimizing water use efficiency and yield fundamentally involves transpiration dynamics, where restriction of maximum transpiration rate helps to avoid early crop failure, while maximizing grain filling. Transpiration restriction can be regulated by multiple mechanisms and involves cross-organ coordination. This coordination involves complex feedbacks and feedforwards over time scales ranging from minutes to weeks, and from spatial scales ranging from cell membrane to crop canopy. Aquaporins have direct effect but various compensation and coordination pathways involve phenology, relative root and shoot growth, shoot architecture, root length distribution profile, as well as other architectural and anatomical aspects of plant form and function. We propose gravimetric phenotyping as an integrative, cross-scale solution to understand the dynamic, interwoven, and context-dependent coordination of transpiration regulation. The most fruitful breeding strategy is likely to be that which maintains focus on the phene of interest, namely, daily and season level transpiration dynamics. This direct selection approach is more precise than yield-based selection but sufficiently integrative to capture attenuating and complementary factors.
Rosa1, a Transposable Element-Like Insertion, Produces Red Petal Coloration in Rose Through Altering RcMYB114 Transcription
Authors: Li Maofu , Zhang Hui , Yang Yuan , Wang Hua , Xue Zhen , Fan Youwei , Sun Pei , Zhang Hong , Zhang Xinzhu , Jin Wanmei
Published: April 2022

Rose (Rosa sp.) flowers have a rich diversity of colors resulting from the differential accumulation of anthocyanins, flavonols, and carotenoids. However, the genetic and molecular determinants of the red-petal trait in roses remains poorly understood. Here we report that a transposable element-like insertion (Rosa1) into RcMYB114, a R2R3-MYB transcription factor’s promoter region causes its transcription, resulting in red petals. In red-petal varieties, RcMYB114 is expressed specifically in flower organs, but is absent from non-red varieties. Sequencing, yeast two-hybrid, transient transformation, and promoter activity assays of RcMYB114 independently confirmed the role of Rosa1 in altering RcMYB114’s transcription and downstream effects on flower color. Genetic and molecular evidence confirmed that the Rosa1 transposable element-like insertion, which is a previously unknown DNA transposable element, is different from those in other plants and is a reliable molecular marker to screen red-petal roses.
Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling
Authors: Martínez-Peña Raquel , Schlereth Armin , Höhne Melanie , Encke Beatrice , Morcuende Rosa , Nieto-Taladriz María Teresa , Araus José Luis , Aparicio Nieves , Vicente Rubén
Published: April 2022

The integration of high-throughput phenotyping and metabolic approaches is a suitable strategy to study the genotype-by-environment interaction and identify novel traits for crop improvement from canopy to an organ level. Our aims were to study the phenotypic and metabolic traits that are related to grain yield and quality at canopy and organ levels, with a special focus on source-sink coordination under contrasting N supplies. Four modern durum wheat varieties with contrasting grain yield were grown in field conditions under two N fertilization levels in north-eastern Spain. We evaluated canopy vegetation indices taken throughout the growing season, physiological and metabolic traits in different photosynthetic organs (flag leaf blade, sheath, peduncle, awn, glume, and lemma) at anthesis and mid-grain filling stages, and agronomic and grain quality traits at harvest. Low N supply triggered an imbalance of C and N coordination at the whole plant level, leading to a reduction of grain yield and nutrient composition. The activities of key enzymes in C and N metabolism as well as the levels of photoassimilates showed that each organ plays an important role during grain filling, some with a higher photosynthetic capacity, others for nutrient storage for later stages of grain filling, or N assimilation and recycling. Interestingly, the enzyme activities and sucrose content of the ear organs were positively associated with grain yield and quality, suggesting, together with the regression models using isotope signatures, the potential contribution of these organs during grain filling. This study highlights the use of holistic approaches to the identification of novel targets to improve grain yield and quality in C3 cereals and the key role of non-foliar organs at late-growth stages.
Identification of Candidate Genes for Lint Percentage and Fiber Quality Through QTL Mapping and Transcriptome Analysis in an Allotetraploid Interspecific Cotton CSSLs Population
Authors: Yang Peng , Sun Xiaoting , Liu Xueying , Wang Wenwen , Hao Yongshui , Chen Lei , Liu Jun , He Hailun , Zhang Taorui , Bao Wanyu , Tang Yihua , He Xinran , Ji Mengya , Guo Kai , Liu Dexin , Teng Zhonghua , Liu Dajun , Zhang Jian , Zhang Zhengsheng
Published: April 2022

Upland cotton (Gossypium hirsutum) has long been an important fiber crop, but the narrow genetic diversity of modern G. hirsutum limits the potential for simultaneous improvement of yield and fiber quality. It is an effective approach to broaden the genetic base of G. hirsutum through introgression of novel alleles from G. barbadense with excellent fiber quality. In the present study, an interspecific chromosome segment substitution lines (CSSLs) population was established using G. barbadense cultivar Pima S-7 as the donor parent and G. hirsutum cultivar CCRI35 as the recipient parent. A total of 105 quantitative trait loci (QTL), including 85 QTL for fiber quality and 20 QTL for lint percentage (LP), were identified based on phenotypic data collected from four environments. Among these QTL, 25 stable QTL were detected in two or more environments, including four for LP, eleven for fiber length (FL), three for fiber strength (FS), six for fiber micronaire (FM), and one for fiber elongation (FE). Eleven QTL clusters were observed on nine chromosomes, of which seven QTL clusters harbored stable QTL. Moreover, eleven major QTL for fiber quality were verified through analysis of introgressed segments of the eight superior lines with the best comprehensive phenotypes. A total of 586 putative candidate genes were identified for 25 stable QTL associated with lint percentage and fiber quality through transcriptome analysis. Furthermore, three candidate genes for FL, GH_A08G1681 (GhSCPL40), GH_A12G2328 (GhPBL19), and GH_D02G0370 (GhHSP22.7), and one candidate gene for FM, GH_D05G1346 (GhAPG), were identified through RNA-Seq and qRT-PCR analysis. These results lay the foundation for understanding the molecular regulatory mechanism of fiber development and provide valuable information for marker-assisted selection (MAS) in cotton breeding.
Development of Wide-Compatible Indica Lines by Pyramiding Multiple Neutral Alleles of Indica–Japonica Hybrid Sterility Loci
Authors: Guo Jie , Li Yun , Xiong Liang , Yan Tingxian , Zou Jinsong , Dai Ziju , Tang Guang , Sun Kangli , Luan Xin , Yang Weifeng , Tan Quanya , Zhu Haitao , Zeng Ruizhen , Wang Shaokui , Zhang Guiquan
Published: April 2022

Since the development of indica hybrid rice in the 1970s, great success has been achieved in hybrid rice production in China and around the world. The utilization of inter-subspecific indica–japonica hybrid rice has always been considered due to its stronger heterosis characteristics. However, indica–japonica hybrids face a serious problem of sterility, which hinders the exploitation of their heterosis. In the past decades, the genetic basis of indica–japonica hybrid sterility has been well studied. It was found that in sterile indica–japonica hybrids, female sterility was mainly controlled by the S5 locus and male sterility by the Sa, Sb, Sc, Sd, and Se loci. In this study, we developed wide-compatible indica lines (WCILs) by pyramiding multiple neutral (n) alleles of the hybrid sterility loci. First, we identified Sn alleles of the loci in single-segment substitution lines (SSSLs) in the genetic background of indica Huajingxian 74 (HJX74). Then, the Sn alleles of S5, Sb, Sc, Sd, and Se loci in SSSLs were pyramided in the HJX74 genetic background. The WCILs carrying Sn alleles at the S5, Sb, Sc, Sd, and Se loci showed wide compatibility with indica and japonica rice varieties. Therefore, the WCILs will be used to develop inter-subspecific indica–japonica hybrid rice with normal fertility.
Oral Tradition as Context for Learning Music From 4E Cognition Compared With Literacy Cultures. Case Studies of Flamenco Guitar Apprenticeship
Authors: Casas-Mas Amalia , Pozo Juan Ignacio , Montero Ignacio
Published: April 2022

The awareness of the last 20 years about embodied cognition is directing multidisciplinary attention to the musical domain and impacting psychological research approaches from the 4E (embodied, embedded, enactive, and extended) cognition. Based on previous research regarding musical teaching and learning conceptions of 30 young guitar apprentices of advanced level in three learning cultures: Western classical, jazz, and flamenco of oral tradition, two participants of flamenco with polarised profiles of learning (reproductive and transformative) were selected as instrumental cases for a prospective ex post facto design. Discourse and practice of the two flamenco guitarists were analysed in-depth to describe bodily issues and verbal discourse on the learning practice in their natural contexts. Qualitative analysis is performed on the posture, gestures, verbal discourse, and musical practice of the participants through the System for the Analysis of Music Teaching and Learning Practices (SAPIL). The results are organised attending: (a) the Embodied mind through differential postures and gestures of flamenco participants that showed a fusion among verbal, body language, and musical discourse with respect to the musical literacy cultures; (b) the Embedded mind and a detailed description of circumstances and relationships of the two flamenco participants, and how music is embedded in their way of life, family and social context, and therefore transcends musical activity itself; (c) the Enactive mind, regarding the active processes that make differences between the reproductive and the transformative flamenco apprentices, then tentative relationship are observed in the discourse of each apprentice and the way in which they practice; finally, (d) the Extended mind through the bodily, technical and symbolic tools they use during learning. Flamenco culture of oral tradition made use of listening, and temporary external representations instead of notational, but also the body played a central role in a holistic rhythm processing through multimodality, such as singing, playing, and dancing. Conclusions point out the embodied mind as a result of the culture of learning reflected through the body and the gesture in instrumental learning.
Not the Master of Your Volitional Mind? The Roles of the Right Medial Prefrontal Cortex and Personality Traits in Unconscious Introjections Versus Self-Chosen Goals
Authors: Quirin Markus , Kerber André , Küstermann Ekkehard , Radtke Elise L. , Kazén Miguel , Konrad Carsten , Baumann Nicola , Ryan Richard M. , Ennis Michael , Kuhl Julius
Published: April 2022

Humans are unconditionally confronted with social expectations and norms, up to a degree that they, or some of them, have a hard time recognizing what they actually want. This renders them susceptible for introjection, that is, to unwittingly or “unconsciously” mistake social expectations for self-chosen goals. Such introjections compromise an individual’s autonomy and mental health and have been shown to be more prevalent in individuals with rumination tendencies and low emotional self-awareness. In this brain imaging study, we draw on a source memory task and found that introjections, as indicated by imposed tasks that are falsely recognized as self-chosen, involved the bilateral medial prefrontal cortex (MPFC) and the dorsal anterior cingulate cortex (ACC). Notably, reduced right MPFC activation within this condition correlated with trait scores of ruminations and reduced emotional self-awareness, but also introversion. Moreover, correct recognition of tasks as self-chosen involved the right MPFC. Accordingly, the right MPFC may play a role in supporting the maintenance of psychological autonomy and counteract introjection, which individuals with certain personality traits seem to be prone to. This research has significant implications for the study of mechanisms underlying autonomous motivation, goal and norm internalization, decision-making, persuasion, education, and clinical conditions such as depression and burnout.
Psychological Distress in Elite Sambo and Recreational Athletes
Authors: Tubić Tatjana , Živanović Bogdan , Lakićević Nemanja , Zenić Nataša , Gilić Barbara , Rudas Eduardas , Eliseev Sergey , Trivić Tatjana B. , Bianco Antonino , Drid Patrik
Published: April 2022

Background

Previous studies suggest that engagement in any type of physical activity can be protective against mental health issues, whereas elite-level athletes can endure various mental health challenges. The aim of this study was to determine variations in the prevalence of psychological distress among elite sambo athletes and their recreational counterparts.

Methods

A sample consisting of 245 athletes (127 males and 118 females) was chosen. Out of the total sample, 105 were elite-level athletes while 140 were recreational athletes. Participants were accessed via the Depression Anxiety Stress Scales-21 to determine their stress in various domains at a given time.

Results

Data indicated that all tested differences between elite sambo athletes and recreational athletes were statistically significant; recreational athletes had a higher score on the depression scale, anxiety and stress, and a general distress score than sambo athletes. Although there are no gender differences in psychological distress in the total sample of athletes, elite sambo athletes achieve significantly lower scores in all tested variants than recreational ones. Women who engage in recreational activities have stood out as a vulnerable subsample in psychological stress.

Conclusion

Future epidemiological and interventional studies should explore optimal strategies to identify mental health needs based on specific sport activity, especially in terms of gender. There is a need to place special emphasis on psychological distress in the context of combat sports.
Optimistic Environmental Messaging Increases State Optimism and in vivo Pro-environmental Behavior
Authors: MacKinnon Megan , Davis Adam C. , Arnocky Steven
Published: April 2022

Despite recent empirical interest, the links between optimism and pessimism with pro-environmental behavior (PEB) remain equivocal. This research is characterized by a reliance on cross-sectional data, a focus on trait-level at the neglect of state-level optimism–pessimism, and assessments of retrospective self-reported ecological behavior that are subject to response bias. To attend to these gaps, 140 North American adults (Mage = 34; SD = 11.60; 44% female) were experimentally primed with bogus optimistic or pessimistic environmental news articles, and then asked to report their levels of state optimism–pessimism, intentions to purchase green products, in vivo PEB (donating to WWF and providing contact information to join an environmental organization), and support for geoengineering technologies. Results confirmed that optimistic (versus pessimistic) environmental messaging enhanced the expression of state optimism, which then contributed to PEB and support for geoengineering. These results have important implications for the framing of environmental messaging intended to promote ecologically conscious behavior.
A Mind in Intelligent Personal Assistants: An Empirical Study of Mind-Based Anthropomorphism, Fulfilled Motivations, and Exploratory Usage of Intelligent Personal Assistants
Authors: Cao Cuicui , Hu Yingying , Xu Haoxuan
Published: April 2022

Intelligent personal assistants (IPAs) own anthropomorphic features which enable users’ perception of anthropomorphism. Adopting the perspective of mind-based anthropomorphism, the purpose of this paper is to investigate how mind-based anthropomorphism influences users’ exploratory usage of IPAs. Based on the notion that anthropomorphism can satisfy people’s sociality and effectance motivation, we hypothesize that mind-based anthropomorphism can enhance people’s social connection with IPAs and IPA self-efficacy, which can in turn influence their exploratory usage of IPAs. Questionnaires were developed and distributed to users who had experience in smart speaker-based IPAs on Wenjuanxing and 551 valid questionnaires were collected to test the research model. The results revealed that cognitive and affective anthropomorphism exerted common and differential impacts on IPA self-efficacy and social connection. Cognitive anthropomorphism versus affective anthropomorphism had stronger influences on IPA self-efficacy, while affective anthropomorphism had stronger impacts on social connection. Both IPA self-efficacy and social connection enhanced users’ intentions to explore IPAs. This study enriches previous studies on IPA adoption or post-adoption by investigating exploratory usage which captures how users are deeply engaged with IPAs.
Validation of the Korean Version of the Anticipatory and Consummatory Interpersonal Pleasure Scale in Non-help-seeking Individuals
Authors: Kim Eunhye , Gooding Diane C. , Lee Tae Young
Published: April 2022

The Anticipatory and Consummatory Interpersonal Pleasure Scale (ACIPS) is a psychometric instrument that has been used to indirectly measure social anhedonia in many cross-cultural contexts, such as in Western (US), European (French, Spanish), Eastern (Chinese), and Israeli samples. However, little is known about the psychometric properties of the ACIPS in Korean samples. The primary goal of this study was to validate the Korean version of the ACIPS among non-help-seeking individuals. The sample consisted of 307 adult individuals who had no current or prior psychiatric history. Participants were administered the ACIPS, along with the Behavioral Inhibition and Behavioral Activation Scales (BIS/BAS) and Beck Depression Inventory (BDI). We examined the association of the total ACIPS scores with the other measures. The ACIPS showed good internal consistency. We also explored the factor structure of the Korean translation of the ACIPS using principal component analysis with Promax rotation and Kaiser normalization. Factor analysis yielded a three-factor structure that accounted for 58.8% of the variance. The three-factor model included the following subdomains: interactions involving close relationships, casual interactions, and interactions involving family members. Total BAS and BIS scores were significantly associated with total ACIPS scores, while BDI scores were inversely associated with total ACIPS scores. The current research indicates that the Korean version of the ACIPS is a useful and valid scale. Future directions include using the Korean translation of the ACIPS to elucidate the varying degrees of hedonic capacity in psychiatric patients.
The Improvement of Teaching Ideological and Political Theory Courses in Universities Based on Immersive Media Technology
Authors: Su Li , Li Mengzuo
Published: April 2022

This paper focuses on the characteristics of immersive media technology and the advantages, problems and solutions in applying this technology to improve the teaching effectiveness of ideological and political theory courses in colleges and universities. Firstly, it introduces the current development and characteristics of immersive media technology. Secondly, it analyzed the outstanding advantages of immersive media technology in teaching from the following perspectives: virtual reality and augmented reality; sensory stimulation and emotional experience; and human-computer interaction and self-harmony. Thirdly, it puts forward the ways to improve the teaching effectiveness based on immersive media technology from the aspects of theoretical study, history study, and practical study of ideological and political theory courses in colleges and universities. Finally, it discusses the problems in applying immersive media technology to teaching the above courses and puts forward some solutions.
User Engagement and User Loyalty Under Different Online Healthcare Community Incentives: An Experimental Study
Authors: Shao Mingxing , Zhao Xinjie , Li Yafang
Published: April 2022

The online healthcare community (OHC) has attained rapid development in recent years in which users are facilitated to exchange disease information and seek medical treatment. However, users’ motivation of participation in OHCs is still under investigation. Taking the perspective of user perceived value, this paper examined the impacts of different incentive levels including identity incentive, privilege incentive, and material incentive on user perceived value, user engagement, and user loyalty. To test the proposed hypotheses, the study adopted the methods of the between-subjects experiment and questionnaire. Based on the data analysis by ANOVA and structural equation model, the results show there are significant differences in the impacts of different incentive levels on users’ perceived value. Most of the incentive measures exert significant effects on simple user hedonic value and community identity value. Accordingly, the research findings suggest that affective support value and self-health management value demonstrate more importance for user engagement and user loyalty. Therefore, OHCs should try to improve users’ affective support value and self-health management value which are the ultimate aims of the OHCs. Our study sheds some light on profoundly understanding the design of incentive mechanism of OHC and contributes to the research of OHC services.