bluuebunny/crossref_metadata_2025 huggingface dataset:
PSIII-18 Assessment of Salmonella Dublin infection of intestinal porcine epithelial cells (IPEC) in response to Zinc Oxide and a Yeast Mannan Rich Fraction
Authors: Browne Niall , Jordan Seána , Horgan Karina
Published: November 2020

Salmonella species are associated with post-weaning diarrhea, which results in poor weight gain, potential death, and economic cost. A yeast mannan rich fraction (MRF) was assessed alongside the industry standard treatment Zinc Oxide (ZO) in vitro to determine its impact on Salmonella Dublin infection of a pig intestinal cell line (IPEC). IPEC cells were exposed to MRF or ZO in the presence of S. Dublin (1x108/mL). IPEC cell RNA was isolated and cDNA synthesized. Gene expression for IL-1β, TNFα, IL-8 and cellular tight Junction genes Occludin, Claudin3 and Tight junction protein1 (TJP1) were assessed by qPCR. S. Dublin adhesion to IPEC cells (500:1) assessed in the presence or absence of either ZO or MRF for 1 hour at 37°C. IPEC cells with attached S. Dublin were lysed, diluted, plated and incubated overnight and enumeration. Three biological replicates were performed for all experiments and statistical analysis determined by One-way ANOVA. Proinflammatory gene TNFα was significantly reduced (P ≤ 0.001) following S. Dublin infection and treatment with MRF compared with ZO. IL-1β demonstrated no change between treatments although IL-8 gene expression was significantly reduced in both ZO (P≤0.01) and MRF (P ≤ 0.05) treated cells over the control. Significantly higher expression of Occludin (P ≤ 0.01), Claudin3 (P ≤ 0.001) and TJP1 (P ≤ 0.05) was observed in IPEC cells exposed to S. Dublin in the presence of MRF compared to ZO. Adhesion of S. Dublin to IPEC cells was significantly reduced in response to MRF addition compared to ZO treated cells (P ≤ 0.001) and the control cells (P ≤ 0.05). ZO treated cell demonstrated no improvement over the control cell levels of bacterial attachment. Both on a physical and molecular level bacterial infection of intestinal cells was more significantly impaired by MRF addition. With the ban on ZO, yeast MRF may prove to be a suitable alternative to support gut health in piglets.
PSII-37 Program Chair Poster Pick: The effect of glucocorticoids on circulating plasma concentrations of ghrelin in sheep
Authors: Kay Carson , Keisler Duane , Daniel Jay A
Published: November 2020

Activation of the hypothalamic-pituitary-adrenal (HPA) axis to release adrenocorticotropic hormone (ACTH) culminates in glucocorticoid production in sheep. Glucocorticoids alter metabolic function, which suggests concentrations of metabolically important hormones, ghrelin and growth hormone (GH), might be affected by components of the HPA axis. To clarify the relationship between the HPA axis and these hormones in ruminants, 10 Katahdin ewe lambs (1 year old; 43.3 +/- 1.3 kg) received an intravenous injection of ACTH (0.2 µg/kg BW) or saline. In a second experiment, the same lambs received an intravenous injection of the synthetic glucocorticoid, dexamethasone (DEX; 2 mg/kg BW) or saline. Blood samples were collected in 15 min intervals from 1 h before injection to 2 h after. Plasma concentrations of cortisol, ghrelin, and GH were analyzed by radioimmunoassay analysis. Data were tested for effect of treatment (saline or ACTH and saline or DEX), time, and treatment by time interaction using procedures for repeated measures with JMP software (version 10; SAS Inst. Inc.). Cortisol concentrations increased in response to both ACTH and dexamethasone administration (P < 0.0001). Neither ghrelin nor GH concentrations were affected by ACTH or DEX administration (P ≥ 0.4372). These data confirm ACTH influences cortisol secretion but suggest neither ACTH nor glucocorticoids impact plasma concentrations of ghrelin and GH in sheep.
PSII-39 Trophectoderm-specific RNA interference of chorionic somatomammotropin alters glucose metabolism in sheep fetal liver
Authors: Ali Asghar , Howerton Mary , Winger Quinton A , Rozance Paul J , Anthony Russell V
Published: November 2020

Chorionic somatomammotropin (CSH) is a placenta-specific hormone and secreted into both fetal and maternal circulation. Reduced maternal CSH is observed with intrauterine growth restriction (IUGR) in both humans and sheep, and it has long been held that CSH modulates maternal and fetal metabolism. We hypothesized that CSH deficiency, created by RNA interference (RNAi), could impact fetal liver glucose metabolism. To generate CSH-deficient pregnancies, day 9 hatched blastocysts were infected with lentiviral particles expressing CSH-specific shRNA (RNAi) or scramble control shRNA (SC) and transferred to synchronized recipients. CSH RNAi generated two distinct phenotypes at 135 dGA; CSH RNAi pregnancies with IUGR (RNAi-IUGR; n = 8) or without IUGR (RNAi; n = 8). Data from both RNAi phenotypes were compared separately with SC using Welch’s t-test. Liver and placental weights were reduced (P < 0.05) in RNAi-IUGR pregnancies, but not in RNAi pregnancies, as compared to SC (n = 8). Umbilical artery plasma insulin and insulin-like growth factor 1 (IGF1) concentrations were decreased (P < 0.05), whereas insulin receptor beta (IRβ) concentration, as determined by Western immunoblot analysis, in fetal liver was increased (P < 0.05) in both RNAi phenotypes. Fetal liver glycogen quantity was also increased (P < 0.05) in both RNAi phenotypes. Glycogen synthase-1 (GYS-1) concentration in fetal liver was increased (P < 0.05) in both RNAi phenotypes, whereas there was no change in GYS-2 concentration. Phosphorylated-GYS (inactive GYS) was reduced (P < 0.05) in fetal livers for both RNAi phenotypes. Lactate dehydrogenase beta (LDHβ) concentration was increased (P < 0.05) and IGF2 concentration was decreased (P < 0.05) in RNAi-IUGR fetal livers only. From these results we conclude that fetal liver glucose metabolism is impacted by CSH RNAi, independent of IUGR, and is likely tied to enhanced insulin sensitivity in both CSH RNAi phenotypes. Differences between the two phenotypes may help differentiate direct and indirect effects of CSH. Supported by NIH R01 HD093701.
PSXI-12 Effect of copper on aminotransferases serum activity in European carp
Authors: Taraskin Artem O , Polistovskaia Polina A , Enukashvili Abram I , Kozitcyna Anna I , Bakhta Alesya A , Balykina Anna B , Karpenko Larisa Y , Plemyashov Kirill
Published: November 2020

The aim of this study is to assess toxic influence of heavy metals in fish. This research was intended to analyze aminotransferases (ALT, AST) serum activity under the influence of various copper concentrations in European carp. Intracellular enzymes activity level elevation in serum is a sign of hepatocites toxic destruction and an indicator of profound cell alterations. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.01 mg/l copper acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.1 mg/l copper acetate concentration (100 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After samplings centrifugation in non-hemolysed serum samples ALT and AST activities were assessed. The study revealed that there was meaningful elevation of ALT and AST serum activity (P ≤ 0.01) by 19.77% (25.68 ± 0,59 IU/l) and 16.84% (264.85 ± 7.6 IU/l) accordingly in 1-experimental group in comparison with control group (21.44 ± 0.72 IU/l и 226.68 ± 4.35 IU/l). In cases where the copper acetate concentration was 100 times higher than fishery waters permissible exposure limit there was meaningful elevation of ALT and AST serum activity (P ≤ 0.01) by 81,62 % (38.94 ± 0.83 IU/l) and 71.81% (389.46 ± 18.7 IU/l) accordingly in 2-experimental group in comparison with control group. After analyzing the received data, there were established meaningful aminotransferases serum activity elevation under the influence of various copper concentrations in European carp. This was possibly due to gluconeogenesis activation in order to support an adequate glucose level in conditions of catabolic process dominance during copper poisoning.
PSXI-13 Effect of performance indicators, food expenses and interest rate on lamb production cost
Authors: Rezende Vanessa , Sartorello Gustavo L , Reis Beatriz Q , Boldrin Guilherme , Alves Laya , Moreno Danny , Souza Miguel , Raineri Camila , Gameiro Augusto H
Published: November 2020

This study aimed at comparing the influence of zootechnical performance and main cost items over the total production cost of lambs. A representative production system of São José do Rio Preto region, Brazil, designed in a panel meeting, was used as a study basis. We calculated lamb production costs, including variable costs, fixed costs, and income of factors, using the data from December 2019. We then estimated elasticities for the variables: feed expense, interest rate, carcass yield, lamb survival rate, and fertility rate. This way we compared the variation in total cost per year and per carcass caused by increases of 1% in each of the considered variables. In the base scenario, production costs per year and unit were, respectively, US$ 33,367.34 and US$ 4.81. Feed expenses, annual interest rate, mean carcass yield, lamb survival rate, and fertility rate were, respectively, US$ 22,316.74, 4.50%, 48.00%, 88.00%, and 85.00%. Regarding annual cost, changing each variable caused variations of +0.67%, +0.05%, 0.00%, +0.33% and +0.33%. Concerning cost per unit, the variations were +0.76%, +0.07%, -0.98%, -0.61% and -0.64%, respectively. Increasing lamb survival and fertility rates were also effective strategies to lower unitary cost. The cost component with feed represented 66.88% of the total production cost, but measures to reduce feed costs must be well conducted to not affect the zootechnical performance of the animals. Farmers must consider cost per unit when thinking about lowering production costs, instead of the annual cost. Increasing lamb carcass yield had a greater impact on reducing unitary cost than cutting feed expenses. Improving performance indicators demand good nutritional status for lambs and ewes, and cutting off feed expenses may have deleterious effects on animal performance, therefore increasing cost. Elasticity analysis is a useful tool to calculate such trade-offs at the farm level.
PSII-8 Different nutritional management strategies post-weaning in growth and reproductive performance of Nellore heifers
Authors: Araújo Laylles C , Prados Laura F , Moriel Philipe , Vasconcelos José Luiz M , Resende Flávio D , Siqueira Gustavo R
Published: November 2020

The objective of this study was to evaluate different nutritional management strategies (drylot vs. supplementation on pasture) to optimize growth and reproductive performance of Nellore heifers submitted to the fixed-time artificial insemination (FTAI) at 14–15 mo. On d0, 126 weaned Nellore heifers (152±22 kg of BW; 240±28 days of age) were stratified by BW and age, and randomly assigned to receive: (1) high-concentrate-based diet (62.2% TDN, 12.0% CP of DM of diet) ad libitum in feedlot from d 0 to 246 (FDL; 6 pens and 7 heifers/pen); (2) fixed supplementation at 1% of BW on Brachiaria pastures from d0 to 246 (PAS10; 6 pastures; 7 heifers/pastures and 4 ha/pasture); and (3) increasing supplementation on Brachiaria pastures at 0.8% of BW from d 0 to 80 and 1.2% of BW from d 81 to 246 (PAS812; 6 pastures; 7 heifers/pastures and 4 ha/pasture). Supplement offered to heifers on pasture consisted of corn, soybean meal, urea, and minerals (78% TDN, 22% CP of DM at dry season and 79% TDN, 20% CP of DM at rainy season). Final pregnancy rate was determined on d238. Heifer BW on d160 was greater (P < 0.01) for FDL vs. PAS10 and PAS812 (264 vs. 251 and 253±10.1 kg, respectively). Overall ADG from d0 to the start of breeding season (d160) and BW on d246 were greater (P < 0.01) for FDL vs. PAS10 and PAS812 (0.72 vs. 0.62 and 0.64±0.02 kg/d, and 325 vs. 310 and 316 ±10.3 kg, respectively). Heifer BW on d160 and 246 and ADG from d0 to 160 did not differ between PAS10 and PAS812 (P ≥ 0.33). Final pregnancy rates did not differ (P = 0.75) among treatments. Therefore, growth performance, but not pregnancy rates following FTAI, was enhanced for Nellore heifers developed in drylot vs. pasture systems and submitted to FTAI at 14–15 mo.
PSII-9 Drylot weaned calves at dry-to-rainy transition and rainy season and their effects on the growing phase
Authors: Nascimento Felipe A , Prados Laura F , Sousa Luciana M , Oliveira Ivanna M , Resende Flávio D , Siqueira Gustavo R
Published: November 2020

The objective of this study was to determine the best nutritional strategy for weaned calves at dry-to-rainy transition and rainy season and their effects on the growing phase. One hundred sixteen Nellore weaned males (initial BW of 173±23 kg; 10 months) were used in a randomized complete block design (blocked by BW) in a 2×2 factorial arrangement of treatments (divided in 12 lots with 9 or 10 animals/lot). Factor 1 was the strategy in the dry-to-rainy season transition (period I; pasture vs. drylot) and factor 2 was the strategy for the rainy season [period II; mineral salt ad libitum (MS) vs. supplement 3 g/kg BW]; consisting of four treatments: pasture in period I and MS in period II; pasture in period I and protein-energy supplement in period II (PEII); drylot in period I and mineral salt in period II; drylot in period I and PEII. At period I, all animals received a protein-energy supplementation (5 g/kg BW). The experiment lasted 244 days. Data were analyzed using the PROC MIXED of SAS. In period I, animals from drylot had highest final BW and ADG (P < 0.01; Table 1). There was no interaction among factors (P ≥ 0.772) in period II. In period II, ADG and carcass gain were highest from pasture animals (P < 0.01). Final BW and HCW were similar (P >0.788) at the end of period II, showing that animals recovery BW. Among strategies at rainy season, animals fed PEII had greater final BW and ADG (P < 0.01). Overall growing phase, HCW and carcass gain were greater for animals fed PEII (P < 0.01). Regardless of the strategy used in the dry-to-rainy season transition, animals were able to equalize final BW at the end of growing phase. In addition, protein-energy supplement at rainy season improves animal performance at the end of growing phase.
PSII-21 Effects of barley starch proportion in diets on nutrient digestion, rumen fermentation parameters and rumen microorganisms of fattening Hu sheep
Authors: Ma Xiaowen , Li Fadi , Li Fei
Published: November 2020

The purpose of this experiment was to study the effects of fattening Hu sheep diet with barley starch instead of corn starch in different proportions on the production performance, nutrient digestion, rumen fermentation parameters and rumen microbial of fattening Hu sheep. In this experiment, 72 Hu lambs (average BW:29.70 ± 1.70 kg) were selected and randomly divided into 4 treatments according to single factor block experiment. Each treatment included 18 replicates(n=18) and each sheep was raised in individual pen. Barely starch (BS) and corn starch (CS) were the starch source of the diets. Four treatments are BS-0 group(0%BS+100%CS), BS-33 group(33%BS+66%CS), BS-66 group(66%BS+33%CS), BS-100 group(100%BS+0%CS). The experiment lasted for 79 days with 7 days pre-feeding period and 63 days formal period(n=18), the last 6 days and 3 days were assigned to the digestibility(n=6) and sampled(n=10) after slaughter respectively. All dates were analyzed using the one-way ANOVE of SPSS 25.0. The results showed that the DM, OM, NDF and ADF digestibility of BS-0 group were higher than BS-100 group (P<0.05). the DM, OM and NDF digestibility of BS-66 group were higher than BS-100 group. Total VFA concentration of BS-66 and BS-100 is significantly higher than BS-33 and BS-0 (P<0.05). the propionate concentration of BS-33 is lower than BS-66 (P<0.05). The ruminal pH and NH3-N concentration of BS-33 are higher than BS-66 (P<0.05).BS-0 group has a higher ACE Chao1 and Shannon index than BS-100. At the phylum level, the abundance of Firmicutes increased and the abundance of Proteobacteria decreased in BS-100(P<0.05). At the genus level, the abundance of Ruminobacter in BS-100 is higher than other groups (P<0.05). These results indicated that the feeding efficiency of corn starch has positive affect than barley starch. 66% barley starch substitutes corn starch can improve nutrient digestibility and be good for rumen health.
PSII-14 Effect of live yeast on greenhouse gas emissions and energy partitioning of growing steers in heat-stress conditions
Authors: D’Souza Genevieve M , Norris Aaron , Batista Luiz Dias , Tedeschi Luis O
Published: November 2020

The objective of this trial was to identify the influence of live yeast supplementation (LY) and temperature exposure (TEMP) on greenhouse gas emissions and energy metabolism of steers receiving a grower diet. The effects of LY and TEMP were investigated using a 2 × 2 crossover design that spanned five periods. Eight Angus crossbred steers (365 ± 32 kg; 41 mo) were randomly split into pairs and housed in four outdoor pens outfitted with an individualized feeding system. Animals were limit fed a grower diet (DIET) at 1.2% SBW with no live yeast supplementation (NOY) or a grower diet top-dressed with 10 g live yeast/d for 14 days (1.2 × 1012 CFU/d). On days 13 and 14, animals were subjected to one of two TEMP conditions, thermoneutral (TN; 18.4 ± 1.1°C, 57.6 ± 2.8 % RH) or heat stress (HS; 33.8 ± 0.6°C, 55.7 ± 2.7 % RH), in two side-by-side, single-stall open-circuit, indirect respiration calorimetry chambers. Data were analyzed using a random coefficients model. Carryover effects were examined and removed from the model if not significant (P > 0.05). There was no effect of DIET, TEMP, or DIET × TEMP (P ≥ 0.752) on enteric methane or carbon dioxide emissions. Similarly, GE, ME, DE, and RE were not influenced by DIET, TEMP, or DIET × TEMP (P ≥ 0.152). Gaseous energy, as a percentage of GE, tended to increase during HS (P = 0.097). Heat energy, as a percentage of GE, had a significant LY carryover effect (P = 0.045), with HS×NOY resulting in less HE than TN×NOY (P = .0495) and HS×LY (P = .084). Live yeast supplementation did not reduce enteric emissions, nor did it improve energy metabolism during heat stress. Further research is required to better understand the dynamic relationship between live yeast and temperature exposure.
PSII-5 Chemical composition, in vitro dry matter digestibility, gas production and methane emission of maintenance, growth and production diets/rations of buffaloes
Authors: Anele Uchenna , Singh Sultan , Kushwaha B P , Gupta P K , Bhattacharya S
Published: November 2020

Ten types of diets were prepared to meet the nutrient requirements of buffaloes for maintenance (MD1 to MD10), growth (GD1 to GD10) and production/lactation (PD1 to PD10). A total of 30 diets were evaluated for chemical composition, in vitro gas and methane production, and dry matter (DM) disappearance using buffalo rumen liquor. The CNCPS was used to estimate the carbohydrate and protein fractions. Protein fractions PB1 and PB2 of maintenance diets were lower (P < 0.05) than growth and production diets, while protein fractions (PB3) and Pc were (P < 0.05) higher in maintenance than in growth and production diets. Mean values of PA was (P < 0.05) higher in growth diets (136.9) than production (114.8) and maintenance diets (105.6 g/kg DM). Maintenance diets had (P < 0.05) higher structural carbohydrate contents (586.2 g/kg DM) than production diets (513.0 g/kg DM). Carbohydrate fraction (CB1) was highest (P < 0.05) in production diets (187.2 g/kg DM) followed by growth (129.5 g/kg DM) and maintenance diets (96.1 g/kg DM). In vitro gas production at different time periods (12, 24 and 48 h) was similar for maintenance (63.04, 51.98 and 48.15 ml/g DM), growth (63.83, 52.73 and 48.250) and production diets (63.51, 52.54 and 47.21 ml/g DM). Cumulative methane production was numerically lower for maintenance (28.40 ml/g DM) than growth (29.58 ml/g DM) and production diets (33.13 ml/g DM). In vitro methane production as a proportion of degraded DM (ml/g DDM and g/kg DDM) was similar for maintenance (14.21 and 29.53), growth (42.19 and 30.25) and production diets (41.26 and 29.58). Dietary chemical constituents such as EE, lignin, NDIN, ADIN and PB3 and Cc were (P < 0.05) negatively associated with methane production, while OM, NPN, SP, PA and PB1, TCHO and CB2 were positively (P < 0.05) correlated with methane production.
PSIX-16 Investigating the development of the fecal microbiome in growing diary calves
Authors: Fowler Emily , St-Pierre Benoit
Published: November 2020

Development of the gut microbiome in young animals is critical for maximizing productivity in adults through beneficial functional contributions of symbiotic microbial communities to the health and nutrition of their host. To gain further insight into this process, development of the fecal microbiome in 12 dairy calves was investigated. Fecal bacterial composition was determined at four time points (weeks 0, 4, 8 and 12) using the 16S rRNA gene through PCR-amplification of the V1-V3 regions from fecal microbial genomic DNA, followed by Illumina MiSeq 2X300 sequencing. A comparative analysis of the most highly represented Operational Taxonomic Units (OTU) using the non-parametric Kruskal-Wallis sum-rank test and Wilcoxon pairwise test identified both known and uncharacterized fecal bacterial species whose abundance fluctuated during development of the calves. Four highly represented OTUs were found to have a peak of abundance at week 0, which was followed by significantly lower abundance at later time points (P < 0.05). Notably, OTU JA_ 89-27339, peaked at week 0 (39.3% ± 3.6%), then declined at later time points with respective means of 2.3%, 0.1% and 0.05%. Seven other OTUs were found to peak at an intermediate time point (P < 0.05), including OTU JA_46-21334 which was found in highest abundance at week 4 (4.5% ± 1.2%) compared to means with a range of 0.001% to 0.01% for the other time points. In contrast, another set of well represented OTUs were found to increase in abundance with time, which included OTU JA_84-17601 whose abundance was highest at week 12 (1.4% ± 0.3%) (P < 0.05). These results are indicative of microbial succession in the gastrointestinal tract of dairy calves and highlight candidate bacterial species whose function could be manipulated towards improving the health and productivity of growing dairy calves.
PSVI-14 Effects of saturated fatty acids of 6 to 12 carbons in length on spoilage microbes, zoonotic pathogens and antimicrobial resistant bacteria in air exposed corn silage
Authors: Salinas-Chavira Jaime , Arzola-Alvarez Claudio , Ruiz-Barrera Oscar , Castillo-Castillo Yamicela , Ontiveros Marina , Jones Barbara W , Smith William B , Hume Michael , Beier Ross C , Harvey Roger , Poole Toni L , Anderson Robin , Nisbet David
Published: November 2020

Aerobic exposure of silage during the feed-out phase promotes growth of spoilage as well as pathogenic and multi-drug resistant (MDR) microbes which can risk infection of food-producing animals and the foods they produce. Livestock producers are interested in finding effective yet environmentally friendly interventions to preserve the nutritional value of these spoiled feedstuffs. We investigated the antimicrobial activity of 6 to 12 carbon-containing saturated fatty acids (C6, C8, C10 or C12) added (0.03 g) individually or as 1:1 mixtures of C6:C8:C10:C12 or C8:C10 to 4 g of overnight exposed silage suspended in 10 mL water. Net changes in colony counts, determined as the difference between counts measured after 0 and 24 h aerobic incubation (22oC) of untreated and treated silage suspensions (n = 3/treatment), were subjected to an analysis of variance. Populations of wild-type total aerobes and experimentally-inoculated MDR-Staphylococcus aureus were unaffected by treatment (P > 0.05), decreasing on average (± SD) 0.64 ± 0.40 and 1.09 ± 0.39 log10 CFU/g silage, respectively. Treatment effects (P < 0.05) were observed against experimentally-inoculated Listeria monocytogenes, with the C8:C10 mix decreasing this foodborne pathogen by 0.64 log10 CFU/g compared to a 0.95 log10/g increase in controls. Indigenous yeast and mold, considered responsible for spoilage, were decreased 2.12 and 3.07 log10 CFU/g by the C8 and C8:C10 treatments compared to 0.40 log10 CFU/g increase in controls. Indigenous lactic acid bacteria, considered beneficial, were decreased most potently by the C8 and C8:C10 treatments compared to controls (1.28, 0.66 and 0.37 log10 CFU/g, respectively). Indigenous enterococci decreased in all incubations, including controls (3.03 log10 CFU/g), compared to 4.69 to 5.03 log10 CFU/g decreases in C6, C8, C10, C12 and C8:C10 treatments. Results reveal that some fatty acid treatments inhibited pathogenic and spoilage microbes, yet treatment optimization is needed to avoid adverse effects against beneficial microbes.
PSVI-3 Effects of dietary supplementation of multi-species direct-fed microbial products on energy status, apparent nutrient digestibility, and rumen metatranscriptome of beef steers
Authors: Ogunade Ibukun M , Pech-Cervantes Andres A , Compart Devan M
Published: November 2020

This study evaluated the effects of two different multi-strain direct-fed microbial products on energy status, nutrient digestibility, and ruminal metatranscriptome of beef steers. Nine rumen-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) PROB (basal diet plus 19 g/d of Commence), and (3) SYNB (basal diet plus 28 g/d of RX3). Commence is a blend of S. cerevisiae, Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei. RX3 is a blend of S. cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis. Rumen fluid (for metatranscriptomics analysis) and blood samples (for analysis of plasma glucose and non-esterified fatty acid) were collected on d 21 of each period. From d 16 – 20, TMR and fecal samples were collected daily to determine apparent total tract digestibility of nutrients using indigestible neutral detergent fiber (iNDF) method. The data were analyzed using the GLIMMIX procedure of SAS. The model included the effects of treatment, period, and random effects of cow and square. There were no effects on DMI and non-esterified fatty acid. Compared with CON, steers fed either additives had greater (P = 0.02) plasma glucose concentrations. Results of metatranscriptome analysis revealed no differentially expressed functional genes among the treatments. Apparent total-tract digestibility of nutrients were also similar among treatments. These results demonstrated that supplemental PROB and SYNB improved the plasma glucose concentration, but had no effects on the functional capacity of the ruminal microbiome and apparent digestibility of nutrients in beef steers.
PSXII-2 Potential interactions between prior endophyte exposure and phytogenic supplementation for finishing steers with varying chute exit velocities
Authors: Shanks Paul A , Vanzant Eric , Caldwell James D
Published: November 2020

All-natural phytogenic supplementation has gained momentum in the feeding industry. Because mechanisms likely differ, these supplements could add benefit when combined with antibiotics in finishing cattle diets. Our objective was to determine influence of Actifor®Energy (ActEN) when fed with monensin and tylosin on performance and carcass measurements of finishing cattle with varying chute exit velocities (EV) and differing prior endophyte exposure (E). Crossbred steers (n = 118; 345 ± 33 kg BW) were utilized in a split plot design (whole plot: prior grazing toxic [EI] or nontoxic [EN] fescue × 6g·hd-1·d-1 dietary ActEN or control). Prior EV (PEV, subplot factor) from receiving, backgrounding, and grazing periods was included as a covariate. Weights and EV were recorded on d0, d28, d56, d112, and at slaughter when cattle were shipped to a commercial abattoir, harvested, and carcass measurements recorded. Starting weights were 22 kg lower (P = 0.10) for cattle previously grazing EI and 10 kg lower (P < 0.01) per 1m·s-1 increase in PEV. Intake (%BW) was unaffected (P > 0.10) by E or ActEN. Gain:feed (E×ActEN P = 0.09) was greater for EN/ActEN compared with EI/ActEN, most influenced by the first 28d. ADG was depressed by EI and increasing PEV (E×PEV P = 0.09) with greater PEV impact for EN compared with EI. Endophyte effects on ADG were most prominent in the first 56d. ActEN tended (P = 0.12) to interact with E status for ADG where gains were numerically greater for ActEN with EN compared with EI, especially during the first 28d. Carcass measurements were largely unaffected (P > 0.10) by E or ActEN. Decreased PEV (P ≤ 0.02) was associated with heavier HCW, increased backfat, and higher YG. Prior exit velocity and grazing regimen were the primary factors influencing performance and carcass traits. Indications of smaller effects of phytogenic supplementation on top of antibiotics, especially during the first 28 d of feeding, warrant further investigation.
PSX-1 A newly developed pectinase from Aspergillus terreus enhanced feed utilization and milk production of Damascus goats fed pectin-rich diet
Authors: Anele Uchenna , Azzaz Hossam , Kholif Ahmed , Murad Hussein , El-Bordeny Nasr , Ebeid Hossam , Hassaan Noha
Published: November 2020

A newly developed pectinase from Aspergillus terreus was compared with a commercially available pectinase at 600 IU/kg feed. Thirty (one-week postpartum) Damascus goats stratified by previous milk production, body weight and parity were divided into three experimental treatments. Does were fed a basal diet containing concentrates, orange silage, sugar beet pulp and wheat straw at 50:20:20:10, respectively with (a newly developed pectinase or commercial pectinase) or without a supplement (control treatment). No difference (P >0.05) was noted for feed intake but the newly developed pectinase increased (P < 0.01) nutrient digestibility, diet nutritive value and milk production efficiency more than the other treatments. Out of all the blood parameters, only serum glucose was affected by the treatments with highest (P = 0.025) value noted for the new pectinase. Similarly, the new pectinase increased daily milk production (P < 0.005) and the concentrations of milk components compared to the other two treatments. Additionally, pectinase (both the commercial and new) inclusion increased (P < 0.05) the concentrations of total conjugated linoleic acid and unsaturated/saturated fatty acids ratio, and decreased atherogenic index (P = 0.01) compared with control treatment. It is concluded that the supplementation of the diet of lactating goats with pectinase at 600 IU/kg feed will enhance feed digestion and milk production. The newly developed pectinase performed better than the commercial pectinase.
PSX-15 Fall grazing of annual forages with fall born lambs may improve animal growth and total protein concentrations when compared to stockpiled pastures
Authors: Campbell Braden J , Gelley Christine , McCutcheon Jefferson S , Fluharty Francis L , Parker Anthony J
Published: November 2020

There is a consistent demand for grass-fed livestock products in the domestic market. Alternative grazing strategies should be considered to sustain the year-round production of grass-fed products that meet consumer demand and support animal health and performance. The objective of this study was to evaluate the effect of annual forages on the growth and parasite resilience of growing lambs. We hypothesized that lambs grazing annual forages would increase their body weight at a greater rate when compared to lambs grazing stockpiled pasture. A total of 54, 60-day-old lambs (24 kg ± 4.9 kg) were stratified by weight, sex, and randomly assigned to one of three grazing treatments: 1) oats (O); 2) turnips (T); and 3) stockpiled tall fescue pasture (F). Lamb body weight and indices of parasitism were measured every 14 days. Data were analyzed using PROC MIXED in SAS. There was a treatment × day effect for body weight such that T lambs were heavier on days 42 and 56 than O and F lambs (P < 0.03). Furthermore, there was a treatment × day effect for average daily gain (ADG) whereas T lambs had a greater ADG on days 28 and 42 than O and F lambs (P < 0.0001). In addition, on day 42, F lambs had a greater ADG when compared to O lambs (P < 0.003). For the indices of parasitism, T lambs demonstrated lesser total plasma protein concentration on days 28 and 42 when compared with O lambs (P < 0.05). Forage type plays a critical role in lamb resilience to gastrointestinal parasites and body weight gain whereas T lambs showed a greater liveweight gain. However, T lambs also demonstrated lesser concentrations of total plasma protein when compared to O lambs. Under these conditions, annual forages adequately sustained the growth of fall grazing lambs.
PSX-25 The distribution of runs of homozygosity in nine native Russian sheep breeds
Authors: Deniskova Tatiana Evgenievna , Abdelmanova Alexandra , Dotsev Arsen V , Reyer Henry , Selionova Marina I , Fornara Margaret S , Wimmers Klaus , Brem Gottfried , Zinovieva Natalia A
Published: November 2020

Native coarse wool fat-tailed sheep breeds are the main producers of mutton in mountainous, steppe and semi-desert regions of Southern Russia. An assessment of homozygosity is required to prevent loss of genetic diversity and inbreeding depression onset. The aim of our work is estimation of genomic inbreeding and distribution of the runs of homozygosity (ROH) segments in Russian sheep breeds. Sample collection included Andean (n = 20), Buryat (n = 18), Buubei (n = 11), Edilbai (n = 20), Karachaev (n = 20), Kalmyk (n = 22), Karakul (n = 20), and Lezgin (n = 15) breeds. All sheep were genotyped using Ovine Infinium® HD SNP BeadChip (Illumina, USA). Quality control and raw data processing were performed in PLINK 1.9. A consecutive runs method implemented in the R package “detectRUNS” was used for estimation of ROH segments. We detected the ROH in all breeds with mean length ranging from 0.35 in Lezgin to 11.18 Mb in Karachaev. Andean had the greatest number of the ROH segments (total of 8496), while 3094 ROH segments were found in Buubei. Short ROH segments (< 2 Mb) were predominant in all breeds and varied from 96.83% in Andean to 99.87% in Buubei. The frequencies of the ROH segments of 8–16 Mb ranged from 0.03% in Karakul to 0.18% in Andean. ROH segments of 8–16 Mb did not occur in the Buubei and Lezgin breeds. The ROH segments were found on all autosomes. The largest genome coverage in the ROH was identified on OAR1, OAR 12 and OAR 20 and the lowest was accounted for OAR 17, OAR 18 and OAR 19. The values FROH ranged from 0.042 in Buubei to 0.084 in Andean. Except of Andean, no traces of long-term inbreeding were detected in local breeds. The obtained data are relevant for future sustainable management of native fat-tailed sheep breeds in Russia. The study was funded by RSF No. 19-16-00070.
PSX-5 Duration of preslaughter stress in winter on physiological responses and meat quality in goats
Authors: Naldurtiker Aditya , Batchu Phaneendra , Estrada-Reyes Zaira , Kouakou Brou , Terrill Thomas H , Kannan Govind
Published: November 2020

Transportation conditions can significantly affect physiological status and meat quality in goats. This experiment was conducted to determine the effects of duration of preslaughter stress on physiological responses and meat quality in goats. Fifty-four uncastrated male Spanish goats (8-mo old; BW = 29.7 ± 2.03 kg) were randomly subjected to one of three treatments (n = 18 goats/treatment): (i) transported for 3 h, (ii) transported for 30 min, or (iii) held in pens (control) on two different days. The ambient temperatures were -3.0 ± 1.0 ºC and 1.0 ± 1.0 ºC on day 1 and 2, respectively. Blood samples were collected before (covariate) and after transport for differential leukocyte count and plasma cortisol concentration determinations. Goats were slaughtered using humane procedures for meat quality analysis. Data were analyzed using PROC GLM in SAS with treatment and day as factors. Cortisol concentrations were higher (P < 0.01) in both 3 h (117.7 ± 7.96 ng/mL) and 30 min (130.1 ± 8.05 ng/mL) transported groups compared to the control group (27.5 ± 7.59 ng/mL). Lymphocyte counts were lower (P < 0.01) in 3 h group compared to the other two groups, and the overall counts were also lower (P < 0.05) on day 1 compared to day 2. The initial Longissimus muscle pH (15 min) was lower on day 1 (6.79 ± 0.04) compared to day 2 (6.94 ± 0.05) and the final pH (24 h) was higher on day 1 (6.31 ± 0.04) than on day 2 (6.14 ± 0.04). Treatment did not influence live or carcass weights, nor did it affect the L*, a*, and b* values of loin chops. The results indicate that ambient temperature during transport may be more important in determining stress responses and muscle pH decline than duration of transportation in goats during winter.
PSVII-26 Detection of selection signatures in Civil and Large White pig breeds
Authors: Getmantseva Lyubov , Bakoev Siroj , Kostyunina Olga V , Traspov Alexey , Prytkov Yuri , Bakoev Nekruz
Published: November 2020

The detection of selection signatures can help to understand the mechanisms of artificial selection. The purpose of this work is to determine the selection signatures in Civil pig relatively Large White breed. The research was carried out on Large White of three breeding centers (LW_1_New=16, LW_2_New=12, LW_3_New=15) and Civilskay, bred in Russia on the basis of local pigs and Large White boars, (Civil=17). GeneSeek GGP Porcine HD BeadChip (San Diego, USA) was used. A heatmap plot of genomic relationship matrix (GRM) was used to evaluate genetic variability between populations. The signals of diversifying selection were detected using Fst and Smoothing Fst (R package Lokern). QTLs and genes were identified and annotated in the Ensembl genome browser (Sscrofa 11.1) (https://www.ensembl.org/index.html), Panther 15.0 (http://www.pantherdb.org/) and QTLdb (https://www.animalgenome.org/cgi-bin/QTLdb/index).Data visualization from heatmapplot (Fig.) showed that all individuals are grouped in relation to their groups. However, the groups LW_1_New, LW_3_New and CIVIL were separated from LW_2_New. We have selected LW_1_New, LW_3_New and CIVIL to identify the selection signatures. After smoothing of the data by moving average, top 0.1% of the observations were considered as pinpointing CIVIL-specific selection signals. As a result, genome regions with strong selection signals on SSC1:214634036-217738857, SSSC2:137533941-138048506, SSC4:24655041-30832595, SSC7:27386432-28655399 and SSC8:55806826-56299366 were found. In these areas, 535 QTLs have been identified, including 347 QTLs for Meat and Carcass Traits (the majority related to Backfat), 67 QTLs Production (Average daily gain or Body weight), 62 QTLs Health (Mean corpuscular volume, Actinobacillus pleuropneumoniae susceptibility et al.), 32 QTLs Reproduction (Teat number) and 27 QTLs Exterior (Leg conformation). In general, 32 genes are defined in these regions (SSC1-11; SSC2-5; SSC4-10; SSC7-3; SSC8-3) (Table). They are mainly involved in the following biological processes: metabolic (GO:0008152), cellular (GO:0009987) and biological regulation (GO:0065007). This research was supported by the Russian Scientific Foundation (RSF) within Project No. 19-16-00109.
PSI-31 Retrospective analysis of growth performance and carcass ultrasound data of yearling bulls enrolled at the Cal Poly Bull Test
Authors: Field Catherine E , Chavez Maria K , Abo-Ismail Mohammed , McFarlane Zach D
Published: November 2020

Since 1956, the Cal Poly Bull Test has evaluated performance of yearling bulls in a multi-breed contemporary group in the western United States. The main objectives of this study were to evaluate the consistency of the relationship between growth and ultrasound traits and between the number of bulls selling and sale price over a 19-year period from 2001–2019. Out of 3,712 bulls, a total of 2,503 Angus and 453 Hereford bulls were used in the analyses using SAS. Bulls were sold in the following pre-determined breed categories; Angus Heifer Bulls, Multi-Trait Angus Bulls, Growth Angus Bulls, and Hereford Bulls. The breed × year interaction significantly (P < 0.01) affected sale price, scrotal circumference (SC), ultrasound ribeye area (UREA), ultrasound intramuscular fat percentage (UIMF), end weight, and average daily gain (ADG). Overall, Hereford bulls had significantly (P < 0.0001) lower ADG than Angus bulls. A positive relationship between ADG and UREA (P < 0.0001; r2 = 0.10) and between UREA and SC (P < 0.0001; r2 = 0.05) was observed. A significant, positive relationship (P < 0.01; r2 = 0.24) between end weight and REA was also exhibited. A significant breed × year interaction (P < 0.01) was observed for sale price, wherein Angus heifer bulls consistently sold for the highest price. Additionally, sale price analyses indicated a moderate, negative relationship (P = 0.02; r2 = -0.584) between number of animals entered in the sale and price. Consistently, Angus bulls had higher (P < 0.0001) sale prices. Sales with higher bull numbers were those with the lowest average prices. The year and breed category explained 31 and 22.5 % of the variance in the price, respectively. Growth performance and sale price may have a biological or genetic component as indicated by breed differences consistently over the 19-year period.
PSI-37 Post-weaning feed efficiency did not influence female fertility traits of commercial or Lowline Angus heifers
Authors: Najera Jonathan , Swanson Rebecca , Warner Andrea L , DeAtley Kasey , Doyle Stephen
Published: November 2020

Feed costs represent ~50% of beef production costs making selection for improved efficiency without affecting female reproduction critical to the cow/calf sector. Objectives were to determine the relationship between post-weaning feed efficiency and female fertility in commercial (n = 19) and half-blood Lowline-influenced Angus heifers (n = 20). Yearling heifers were delivered to the CSU, Chico Farm cattle feeding unit 45-d post weaning and randomly assigned to one of three 7 x 18 m pens, equipped with GrowSafe feeding units. Heifers were given ad libitum access to water and a forage-based diet targeting 0.68 kg gain/d (TDN: 54%, CP: 14%, and NDF: 39.7%, on a DM basis) for 72-d. Feed efficiency measures included dry matter intake (DMI), feed conversion ratio (FCR) and residual feed intake (RFI). Heifer RFI was calculated as the difference between predicted DMI and actual DMI during the trial. Feed efficiency groups were identified as: 1) low RFI (0.5 SD above the RFI mean), 2) marginal RFI (+/- 0.5 SD about the RFI mean), and 3) high RFI (0.5 SD above the RFI mean). Serial weights were taken every 14-d to calculate average daily gain (ADG) and metabolic midweight (MMWT). Heifers were synchronized using a 14-d CIDR-PG protocol. Heifers received PG injection 16-d post-CIDR removal and were equipped with an estrous detection patch. Estrous behavior was observed morning and evening for 90-h. Heifers were artificially inseminated (AI) 12-h post-standing estrus and injected with 2 mL GnRH. Pregnancy determination via rectal palpation occurred 6-mos after initial AI. Reproductive measures included: first service conception (FSC), pregnancy rate (PR), and response to estrous synchronization (RES). Reproductive traits were analyzed using the GLM procedures of SPSS with a binary logistic response. Breed type and feed efficiency group were independent variables. Relationships among reproductive measures and performance were analyzed using logistic regression of SPSS, fitting the independent variables of ADG, DMI, and RFI. Traits FSC, PR, and RES did not differ among breed types or feed efficiency groups (P > 0.05) and DMI, ADG and RFI were not predictors of FSC, PR, nor RES (P > 0.05). Cumulatively, results indicate there is no relationship between feed efficiency and reproductive performance. Producers can select for low RFI to decrease feed costs without compromising reproductive performance.
Post-marketing surveillance study of trifluridine/tipiracil in patients with metastatic colorectal cancer
Authors: Yoshino Takayuki , Uetake Hiroyuki , Funato Yuya , Yamaguchi Yasunobu , Koyama Takahiko , Ozawa Daisuke , Tajiri Masaru , Muro Kei
Published: November 2020

Background

The novel oral nucleoside antineoplastic agent trifluridine/tipiracil was approved for metastatic colorectal cancer in Japan in March 2014. In this post-marketing surveillance study, we investigated the safety and efficacy of trifluridine/tipiracil in a real-world setting, particularly haematological drug reactions classified according to the baseline renal and hepatic functions.

Methods

We investigated patients with metastatic colorectal cancer who received trifluridine/tipiracil during the first four treatment cycles prospectively. The patients typically received 35 mg/m2 trifluridine/tipiracil twice daily on days 1–5 and 8–12 every 28 days. The primary objective was to assess the safety of trifluridine/tipiracil, but its efficacy was also evaluated.

Results

Between July 2014 and June 2016, 860 patients were enrolled in the study, and the safety and efficacy of trifluridine/tipiracil were evaluated in 823 patients. Adverse drug reactions occurred in 89.7% of the patients. The most common adverse drug reactions were decreased white blood cell count (67.0%) and neutrophil count (63.9%). Haematological drug reactions of grade ≥3 were observed in 41.7% of the patients with normal renal function; 50.3, 65.6 and 78.9% of the patients had mild, moderate and severe renal impairments, respectively. Hepatic impairment was not associated with a higher incidence of haematological drug reactions. The median overall survival was 8.4 months, with a 1-year survival rate of 33.7%.

Conclusion

This post-marketing surveillance study further confirmed the safety and tolerability profile of trifluridine/tipiracil observed in a clinical study setting.
Exposure to Tobacco Smoke and Temperament among U.S. Children 0–5 Years Old
Authors: Merianos Ashley L , Nabors Laura A , Fiser Kayleigh A , Mahabee-Gittens E Melinda
Published: November 2020

Objectives

This study examined the association between tobacco smoke exposure (TSE) and temperament among children 0–5 years old overall and within age groups: 0–2 and 3–5 years.

Methods

Data were obtained from the 2017–2018 NSCH (N = 14,345). TSE status was defined as whether children lived with a smoker who: does not smoke inside the home (no home TSE) or smokes inside the home (home TSE). We conducted logistic regression analyses while controlling for covariates.

Results

Overall, 12.5% of children lived with a smoker with no home TSE and 1.1% had home TSE. Children with home TSE were at increased odds to not always: be affectionate and tender (aOR = 1.74, 95% CI = 1.18–2.58), show interest and curiosity (aOR = 1.81, 95% CI = 1.23–2.68), and smile and laugh (aOR = 1.77, 95% CI = 1.13–2.77) than those with no TSE. Among 0- to 2-year-olds, those with home TSE were more likely to not always be affectionate and tender (aOR = 1.97, 95% CI = 1.04–3.74). Among 3- to 5-year-olds, those who lived with a smoker with no home TSE were more likely to not always: bounce back quickly (aOR = 1.21, 95% CI = 1.05–1.40) and smile and laugh (aOR = 1.26, 95% CI = 1.03–1.54), and those with home TSE were more likely to not always: show interest and curiosity (aOR = 2.24, 95% CI = 1.40–3.59) and smile and laugh (aOR = 2.43, 95% CI = 1.43–4.11).

Conclusions

Tobacco smoke-exposed children were at increased odds of not always demonstrating positive early childhood temperament behaviors, with 3- to 5-year-olds having more pronounced odds.