bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatial Context-Based Local Toponym Extraction and Chinese Textual Address Segmentation from Urban POI Data
Authors: Kuai Xi , Guo Renzhong , Zhang Zhijun , He Biao , Zhao Zhigang , Guo Han
Published: March 2020

Georeferencing by place names (known as toponyms) is the most common way of associating textual information with geographic locations. While computers use numeric coordinates (such as longitude-latitude pairs) to represent places, people generally refer to places via their toponyms. Query by toponym is an effective way to find information about a geographic area. However, segmenting and parsing textual addresses to extract local toponyms is a difficult task in the geocoding field, especially in China. In this paper, a local spatial context-based framework is proposed to extract local toponyms and segment Chinese textual addresses. We collect urban points of interest (POIs) as an input data source; in this dataset, the textual address and geospatial position coordinates correspond at a one-to-one basis and can be easily used to explore the spatial distribution of local toponyms. The proposed framework involves two steps: address element identification and local toponym extraction. The first step identifies as many address element candidates as possible from a continuous string of textual addresses for each urban POI. The second step focuses on merging neighboring candidate pairs into local toponyms. A series of experiments are conducted to determine the thresholds for local toponym extraction based on precision-recall curves. Finally, we evaluate our framework by comparing its performance with three well-known Chinese word segmentation models. The comparative experimental results demonstrate that our framework achieves a better performance than do other models.
Oral Microbes and Mucosal Dendritic Cells, “Spark and Flame” of Local and Distant Inflammatory Diseases
Authors: Meghil Mohamed M. , Cutler Christopher W.
Published: March 2020

Mucosal health and disease is mediated by a complex interplay between the microbiota (“spark”) and the inflammatory response (“flame”). Pathobionts, a specific class of microbes, exemplified by the oral microbe Porphyromonas gingivalis, live mostly “under the radar” in their human hosts, in a cooperative relationship with the indigenous microbiota. Dendritic cells (DCs), mucosal immune sentinels, often remain undisturbed by such microbes and do not alert adaptive immunity to danger. At a certain tipping point of inflammation, an “awakening” of pathobionts occurs, wherein their active growth and virulence are stimulated, leading to a dysbiosis. Pathobiont becomes pathogen, and commensal becomes accessory pathogen. The local inflammatory outcome is the Th17-mediated degenerative bone disease, periodontitis (PD). In systemic circulation of PD subjects, inflammatory DCs expand, carrying an oral microbiome and promoting Treg and Th17 responses. At distant peripheral sites, comorbid diseases including atherosclerosis, Alzheimer’s disease, macular degeneration, chronic kidney disease, and others are reportedly induced. This review will review the immunobiology of DCs, examine the complex interplay of microbes and DCs in the pathogenesis of PD and its comorbid inflammatory diseases, and discuss the role of apoptosis and autophagy in this regard. Overall, the pathophysiological mechanisms of DC-mediated chronic inflammation and tissue destruction will be summarized.
LPIAT, a lyso-Phosphatidylinositol Acyltransferase, Modulates Seed Germination in Arabidopsis thaliana through PIP Signalling Pathways and is Involved in Hyperosmotic Response
Authors: Coulon Denis , Faure Lionel , Grison Magali , Pascal Stéphanie , Wattelet-Boyer Valérie , Clark Jonathan , Le Guedard Marina , Testet Eric , Bessoule Jean-Jacques
Published: March 2020

Lyso-lipid acyltransferases are enzymes involved in various processes such as lipid synthesis and remodelling. Here, we characterized the activity of an acyltransferase from Arabidopsis thaliana (LPIAT). In vitro, this protein, expressed in Escherichia coli membrane, displayed a 2-lyso-phosphatidylinositol acyltransferase activity with a specificity towards saturated long chain acyl CoAs (C16:0- and C18:0-CoAs), allowing the remodelling of phosphatidylinositol. In planta, LPIAT gene was expressed in mature seeds and very transiently during seed imbibition, mostly in aleurone-like layer cells. Whereas the disruption of this gene did not alter the lipid composition of seed, its overexpression in leaves promoted a strong increase in the phosphatidylinositol phosphates (PIP) level without affecting the PIP2 content. The spatial and temporal narrow expression of this gene as well as the modification of PIP metabolism led us to investigate its role in the control of seed germination. Seeds from the lpiat mutant germinated faster and were less sensitive to abscisic acid (ABA) than wild-type or overexpressing lines. We also showed that the protective effect of ABA on young seedlings against dryness was reduced for lpiat line. In addition, germination of lpiat mutant seeds was more sensitive to hyperosmotic stress. All these results suggest a link between phosphoinositides and ABA signalling in the control of seed germination
Immune Checkpoint Inhibitors in Esophageal Cancers: Are We Finally Finding the Right Path in the Mist?
Authors: Vivaldi Caterina , Catanese Silvia , Massa Valentina , Pecora Irene , Salani Francesca , Santi Stefano , Lencioni Monica , Vasile Enrico , Falcone Alfredo , Fornaro Lorenzo
Published: March 2020

Esophageal cancer remains a challenging disease due to limited treatment options and poor prognosis. In recent years, immune checkpoint inhibitors (ICI) have been proven to be safe and effective in the treatment of highly lethal malignancies, such as non-small cell lung cancer and melanoma. Recent clinical trials also showed promising activity in immune checkpoint inhibitors in pretreated advanced esophageal carcinoma and a potentially significant impact on the outcome of selected patients, independently of histology. Combination studies evaluating immunotherapy and chemotherapy and, in localized disease, radiotherapy are in progress and will hopefully confirm their promises in the near future. However, reliable predictive biomarkers are still lacking. Indeed, at present, the role of programmed cell death ligand 1 expression and other factors (such as microsatellite instability and tumor mutational burden) as predictive biomarkers of benefit to immune checkpoint inhibitors is still controversial. Our aim was to explore the rationale of ICIs in esophageal cancer, review the results already available in multiple settings, and investigate future perspectives with single-agent and combination strategies.
METTL3 Modulates Osteoclast Differentiation and Function by Controlling RNA Stability and Nuclear Export
Authors: Li Di , Cai Luhui , Meng Runsha , Feng Zhihui , Xu Qiong
Published: March 2020

Osteoclast differentiation and function are crucial for maintaining bone homeostasis and preserving skeletal integrity. N6-methyladenosine (m6A) is an abundant mRNA modification that has recently been shown to be important in regulating cell lineage differentiation. Nevertheless, the effect of m6A on osteoclast differentiation remains unknown. In the present study, we observed that the m6A level and methyltransferase METTL3 expression increased during osteoclast differentiation. Mettl3 knockdown resulted in an increased size but a decreased bone-resorbing ability of osteoclasts. The expression of osteoclast-specific genes (Nfatc1, c-Fos, Ctsk, Acp5 and Dcstamp) was inhibited by Mettl3 depletion, while the expression of the cellular fusion-specific gene Atp6v0d2 was upregulated. Mechanistically, Mettl3 knockdown elevated the mRNA stability of Atp6v0d2 and the same result was obtained when the m6A-binding protein YTHDF2 was silenced. Moreover, the phosphorylation levels of key molecules in the MAPK, NF-κB and PI3K-AKT signaling pathways were reduced upon Mettl3 deficiency. Depletion of Mettl3 maintained the retention of Traf6 mRNA in the nucleus and reduced the protein levels of TRAF6. Taken together, our data suggest that METTL3 regulates osteoclast differentiation and function through different mechanisms involving Atp6v0d2 mRNA degradation mediated by YTHDF2 and Traf6 mRNA nuclear export. These findings elucidate the molecular basis of RNA epigenetic regulation in osteoclast development.
Into the Seed: Auxin Controls Seed Development and Grain Yield
Authors: Cao Jinshan , Li Guoji , Qu Dejie , Li Xia , Wang Youning
Published: March 2020

Seed development, which involves mainly the embryo, endosperm and integuments, is regulated by different signaling pathways, leading to various changes in seed size or seed weight. Therefore, uncovering the genetic and molecular mechanisms of seed development has great potential for improving crop yields. The phytohormone auxin is a key regulator required for modulating different cellular processes involved in seed development. Here, we provide a comprehensive review of the role of auxin biosynthesis, transport, signaling, conjugation, and catabolism during seed development. More importantly, we not only summarize the research progress on the genetic and molecular regulation of seed development mediated by auxin but also discuss the potential of manipulating auxin metabolism and its signaling pathway for improving crop seed weight.
Differential Inhibitory Actions of Multitargeted Tyrosine Kinase Inhibitors on Different Ionic Current Types in Cardiomyocytes
Authors: Chang Wei-Ting , Liu Ping-Yen , Lee Kaisen , Feng Yin-Hsun , Wu Sheng-Nan
Published: March 2020

Lapatinib (LAP) and sorafenib (SOR) are multitargeted tyrosine kinase inhibitors (TKIs) with antineoplastic properties. In clinical observations, LAP and SOR may contribute to QTc prolongation, but the detailed mechanism for this has been largely unexplored. In this study, we investigated whether LAP and SOR affect the activities of membrane ion channels. Using a small animal model and primary cardiomyocytes, we studied the impact of LAP and SOR on Na+ and K+ currents. We found that LAP-induced QTc prolongation in mice was reversed by isoproterenol. LAP or SOR suppressed the amplitude of the slowly activating delayed-rectifier K+ current (IK(S)) in H9c2 cells in a time- and concentration-dependent fashion. The LAP-mediated inhibition of IK(S) was reversed by adding isoproterenol or meclofenamic acid, but not by adding diazoxide. The steady-state activation curve of IK(S) during exposure to LAP or SOR was shifted toward a less negative potential, with no change in the gating charge required to activate the current. LAP shortened the recovery from IK(S) deactivation. As rapid repetitive stimuli, the IK(S) amplitude decreased; however; the LAP-induced inhibition of IK(S) remained effective. LAP or SOR alone also suppressed inwardly rectifying K+ and voltage-gated Na+ current in neonatal rat ventricular myocytes. The inhibition of ionic currents during exposure to TKIs could be an important mechanism underlying changes in QTc intervals.
The Binding of Aβ42 Peptide Monomers to Sphingomyelin/Cholesterol/Ganglioside Bilayers Assayed by Density Gradient Ultracentrifugation
Authors: Ahyayauch Hasna , de la Arada Igor , Masserini Massimo E. , Arrondo José L. R. , Goñi Félix M. , Alonso Alicia
Published: March 2020

The binding of Aβ42 peptide monomers to sphingomyelin/cholesterol (1:1 mol ratio) bilayers containing 5 mol% gangliosides (either GM1, or GT1b, or a mixture of brain gangliosides) has been assayed by density gradient ultracentrifugation. This procedure provides a direct method for measuring vesicle-bound peptides after non-bound fraction separation. This centrifugation technique has rarely been used in this context previously. The results show that gangliosides increase by about two-fold the amount of Aβ42 bound to sphingomyelin/cholesterol vesicles. Complementary studies of the same systems using thioflavin T fluorescence, Langmuir monolayers or infrared spectroscopy confirm the ganglioside-dependent increased binding. Furthermore these studies reveal that gangliosides facilitate the aggregation of Aβ42 giving rise to more extended β-sheets. Thus, gangliosides have both a quantitative and a qualitative effect on the binding of Aβ42 to sphingomyelin/cholesterol bilayers.
The Yin and Yang of Autosomal Recessive Primary Microcephaly Genes: Insights from Neurogenesis and Carcinogenesis
Authors: Zhou Xiaokun , Zhi Yiqiang , Yu Jurui , Xu Dan
Published: March 2020

The stem cells of neurogenesis and carcinogenesis share many properties, including proliferative rate, an extensive replicative potential, the potential to generate different cell types of a given tissue, and an ability to independently migrate to a damaged area. This is also evidenced by the common molecular principles regulating key processes associated with cell division and apoptosis. Autosomal recessive primary microcephaly (MCPH) is a neurogenic mitotic disorder that is characterized by decreased brain size and mental retardation. Until now, a total of 25 genes have been identified that are known to be associated with MCPH. The inactivation (yin) of most MCPH genes leads to neurogenesis defects, while the upregulation (yang) of some MCPH genes is associated with different kinds of carcinogenesis. Here, we try to summarize the roles of MCPH genes in these two diseases and explore the underlying mechanisms, which will help us to explore new, attractive approaches to targeting tumor cells that are resistant to the current therapies.
The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia
Authors: Yeh Lu-Chen , Chen Shu-Ping , Liao Fang-Hsuean , Wu Te-Haw , Huang Yu-Ting , Lin Shu-Yi
Published: March 2020

The progress of wound regeneration relies on inflammation management, while neovascular angiogenesis is a critical aspect of wound healing. In this study, the bioactive core and corona synergism of quantized gold (QG) were developed to simultaneously address these complicated issues, combining the abilities to eliminate endotoxins and provide oxygen. The QG was constructed from ultrasmall nanogold and a loosely packed amine-based corona via a simple process, but it could nonetheless eliminate endotoxins (a vital factor in inflammation also called lipopolysaccharides) and provide oxygen in situ for the remodeling of wound sites. Even while capturing endotoxins through electrostatic interactions, the catalytic active sites inside the nanogold could maintain its surface accessibility to automatically transform the overexpressed hydrogen peroxide in hypoxic wound regions into oxygen. Since the inflammatory stage is an essential stage of wound healing, the provision of endotoxin clearance by the outer organic corona of the QG could slow inflammation in a way that subsequently promoted two other important stages of wound bed healing, namely proliferation and remodeling. Relatedly, the efficacy of two forms of the QG, a liquid form and a dressing form, was demonstrated at wound sites in this study, with both forms promoting the development of granulation, including angiogenesis and collagen deposition. Thus, the simply fabricated dual function nanocomposite presented herein not only offers reduced batch-to-batch variation but also increased options for homecare treatments.
Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling
Authors: Bellich Barbara , Jou Ining A. , Caterino Marco , Rizzo Roberto , Ravenscroft Neil , Fazli Mustafa , Tolker-Nielsen Tim , Brady John W. , Cescutti Paola
Published: March 2020

Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells. Extracellular polysaccharides play an important role in matrix setup and maintenance. A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen. Its composition and glycosidic linkages were determined using Gas–Liquid Chromatography–Mass Spectrometry (GLC–MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions. All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility. Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions. In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.
Visualizing the Synaptic and Cellular Ultrastructure in Neurons Differentiated from Human Induced Neural Stem Cells—An Optimized Protocol
Authors: Capetian Philipp , Müller Lorenz , Volkmann Jens , Heckmann Manfred , Ergün Süleyman , Wagner Nicole
Published: March 2020

The size of the synaptic subcomponents falls below the limits of visible light microscopy. Despite new developments in advanced microscopy techniques, the resolution of transmission electron microscopy (TEM) remains unsurpassed. The requirements of tissue preservation are very high, and human post mortem material often does not offer adequate quality. However, new reprogramming techniques that generate human neurons in vitro provide samples that can easily fulfill these requirements. The objective of this study was to identify the culture technique with the best ultrastructural preservation in combination with the best embedding and contrasting technique for visualizing neuronal elements. Two induced neural stem cell lines derived from healthy control subjects underwent differentiation either adherent on glass coverslips, embedded in a droplet of highly concentrated Matrigel, or as a compact neurosphere. Afterward, they were fixed using a combination of glutaraldehyde (GA) and paraformaldehyde (PFA) followed by three approaches (standard stain, Ruthenium red stain, high contrast en-bloc stain) using different combinations of membrane enhancing and contrasting steps before ultrathin sectioning and imaging by TEM. The compact free-floating neurospheres exhibited the best ultrastructural preservation. High-contrast en-bloc stain offered particularly sharp staining of membrane structures and the highest quality visualization of neuronal structures. In conclusion, compact neurospheres growing under free-floating conditions in combination with a high contrast en-bloc staining protocol, offer the optimal preservation and contrast with a particular focus on visualizing membrane structures as required for analyzing synaptic structures.
Arabidopsis RETICULON-LIKE4 (RTNLB4) Protein Participates in Agrobacterium Infection and VirB2 Peptide-Induced Plant Defense Response
Authors: Huang Fan-Chen , Hwang Hau-Hsuan
Published: March 2020

Agrobacterium tumefaciens uses the type IV secretion system, which consists of VirB1-B11 and VirD4 proteins, to deliver effectors into plant cells. The effectors manipulate plant proteins to assist in T-DNA transfer, integration, and expression in plant cells. The Arabidopsis reticulon-like (RTNLB) proteins are located in the endoplasmic reticulum and are involved in endomembrane trafficking in plant cells. The rtnlb4 mutants were recalcitrant to A. tumefaciens infection, but overexpression of RTNLB4 in transgenic plants resulted in hypersusceptibility to A. tumefaciens transformation, which suggests the involvement of RTNLB4 in A. tumefaciens infection. The expression of defense-related genes, including FRK1, PR1, WRKY22, and WRKY29, were less induced in RTNLB4 overexpression (O/E) transgenic plants after A. tumefaciens elf18 peptide treatment. Pretreatment with elf18 peptide decreased Agrobacterium-mediated transient expression efficiency more in wild-type seedlings than RTNLB4 O/E transgenic plants, which suggests that the induced defense responses in RTNLB4 O/E transgenic plants might be affected after bacterial elicitor treatments. Similarly, A. tumefaciens VirB2 peptide pretreatment reduced transient T-DNA expression in wild-type seedlings to a greater extent than in RTNLB4 O/E transgenic seedlings. Furthermore, the VirB2 peptides induced FRK1, WRKY22, and WRKY29 gene expression in wild-type seedlings but not efr-1 and bak1 mutants. The induced defense-related gene expression was lower in RTNLB4 O/E transgenic plants than wild-type seedlings after VirB2 peptide treatment. These data suggest that RTNLB4 may participate in elf18 and VirB2 peptide-induced defense responses and may therefore affect the A. tumefaciens infection process.
Tree-Like Distributed Computation Environment with Shapp Library
Authors: Gałecki Tomasz , Daszczuk Wiktor Bohdan
Published: March 2020

Despite the rapidly growing computing power of computers, it is often insufficient to perform mass calculations in a short time, for example, simulation of systems for various sets of parameters, the searching of huge state spaces, optimization using ant or genetic algorithms, machine learning, etc. One can solve the problem of a lack of computing power through workload management systems used in local networks in order to use the free computing power of servers and workstations. This article proposes raising such a system to a higher level of abstraction: The use in the .NET environment of a new Shapp library that allows remote task execution using fork-like operations from Portable Operating System Interface for UNIX (POSIX) systems. The library distributes the task code, sending static data on which task force is working, and individualizing tasks. In addition, a convenient way of communicating distributed tasks running hierarchically in the Shapp library was proposed to better manage the execution of these tasks. Many different task group architectures are possible; we focus on tree-like calculations that are suitable for many problems where the range of possible parallelism increases as the calculations progress.
Serious Games, Mental Images, and Participatory Mapping: Reflections on a Set of Enabling Tools for Capacity Building
Authors: Constantinescu Teodora Iulia , Devisch Oswald
Published: March 2020

Increasing complexity of societal questions requires participatory processes that engage with capable participants. We adopted Horellis’ stance on participation as not an isolated event but a constant communication between different groups that can be assured by using enabling tools. We applied the Capability Approach to frame a capacity-building process and understand how this framework can support a collective of entrepreneurs to become aware of their capabilities (and the impact of an ongoing urban renewal process on these capabilities). The Capability Approach emphasizes the personal and structural conditions that impact a person’s capability to choose—the conditions that affect the process of determining what a person values. The paper builds on a two year capacity-building process conducted in Genk, Belgium, and proposes a conceptual framework for building capacities, in which the process and outputs collide with ideas of choice, ability, and opportunity, notions central to the Capability Approach. The case study looks at one of the main commercial streets of the city (Vennestraat) and reflects on a set of enabling artefacts used to engage proprietors in the capacity-building process. This capacity-building process, characterized by the idea of space and capabilities, advances a critical viewpoint on issues related to participatory processes and gives practitioners a set of enabling tools to start a conversation over complex urban transformations, such as the one in Vennestraat.
A Review of the MSCA ITN ECOSTORE—Novel Complex Metal Hydrides for Efficient and Compact Storage of Renewable Energy as Hydrogen and Electricity
Authors: Hadjixenophontos Efi , Dematteis Erika Michela , Berti Nicola , Wołczyk Anna Roza , Huen Priscilla , Brighi Matteo , Le Thi Thu , Santoru Antonio , Payandeh SeyedHosein , Peru Filippo , Dao Anh Ha , Liu Yinzhe , Heere Michael
Published: March 2020

Hydrogen as an energy carrier is very versatile in energy storage applications. Developments in novel, sustainable technologies towards a CO2-free society are needed and the exploration of all-solid-state batteries (ASSBs) as well as solid-state hydrogen storage applications based on metal hydrides can provide solutions for such technologies. However, there are still many technical challenges for both hydrogen storage material and ASSBs related to designing low-cost materials with low-environmental impact. The current materials considered for all-solid-state batteries should have high conductivities for Na+, Mg2+ and Ca2+, while Al3+-based compounds are often marginalised due to the lack of suitable electrode and electrolyte materials. In hydrogen storage materials, the sluggish kinetic behaviour of solid-state hydride materials is one of the key constraints that limit their practical uses. Therefore, it is necessary to overcome the kinetic issues of hydride materials before discussing and considering them on the system level. This review summarizes the achievements of the Marie Skłodowska-Curie Actions (MSCA) innovative training network (ITN) ECOSTORE, the aim of which was the investigation of different aspects of (complex) metal hydride materials. Advances in battery and hydrogen storage materials for the efficient and compact storage of renewable energy production are discussed.
Characteristics of the Two Asian Bumblebee Species Bombus friseanus and Bombus breviceps (Hymenoptera: Apidae)
Authors: Liang Cheng , Ding Guiling , Huang Jiaxing , Zhang Xuewen , Miao Chunhui , An Jiandong
Published: March 2020

This study compared the food plants, life cycle, colony development, and mating behaviour of the two Asian bumblebee species Bombus friseanus and B. breviceps, which are very important pollinators for many wild flowers and crops in local ecosystems. Both species were shown to be highly polylectic. Differences were observed in their life cycles and colony development patterns. The colony foundation rate of the field-collected queens was high in both species, 95.5% in B. friseanus and 86.5% in B. breviceps. The intervals from colony initiation to colony sizes of 30, 60, and 80 workers and to the first male and gyne emergence were significantly shorter in B. friseanus than in B. breviceps (p < 0.01). The development period of the first batch of workers showed no significant difference between the two species (p > 0.05). Compared with B. friseanus, B. breviceps produced remarkably higher numbers of workers (135 ± 30 workers/colony in B. friseanus and 318 ± 123 workers/colony in B. breviceps) and males (199 ± 46 males/colony in B. friseanus and 355 ± 166 males/colony in B. breviceps) (p < 0.01), with notable variation was found among the colonies in both species. With no significant difference in the mating rate between these two species, the copulation duration of B. breviceps (1.54 ± 0.63 min) was strikingly shorter than that of B. friseanus (27.44 ± 11.16 min) (p < 0.001). This study highlights the characteristics of the two Asian bumblebee species and will aid further studies on their conservation and agricultural pollination use.
Optimization Method for Forecasting Confirmed Cases of COVID-19 in China
Authors: Al-qaness Mohammed A. A. , Ewees Ahmed A. , Fan Hong , Abd El Aziz Mohamed
Published: March 2020

In December 2019, a novel coronavirus, called COVID-19, was discovered in Wuhan, China, and has spread to different cities in China as well as to 24 other countries. The number of confirmed cases is increasing daily and reached 34,598 on 8 February 2020. In the current study, we present a new forecasting model to estimate and forecast the number of confirmed cases of COVID-19 in the upcoming ten days based on the previously confirmed cases recorded in China. The proposed model is an improved adaptive neuro-fuzzy inference system (ANFIS) using an enhanced flower pollination algorithm (FPA) by using the salp swarm algorithm (SSA). In general, SSA is employed to improve FPA to avoid its drawbacks (i.e., getting trapped at the local optima). The main idea of the proposed model, called FPASSA-ANFIS, is to improve the performance of ANFIS by determining the parameters of ANFIS using FPASSA. The FPASSA-ANFIS model is evaluated using the World Health Organization (WHO) official data of the outbreak of the COVID-19 to forecast the confirmed cases of the upcoming ten days. More so, the FPASSA-ANFIS model is compared to several existing models, and it showed better performance in terms of Mean Absolute Percentage Error (MAPE), Root Mean Squared Relative Error (RMSRE), Root Mean Squared Relative Error (RMSRE), coefficient of determination ( R 2 ), and computing time. Furthermore, we tested the proposed model using two different datasets of weekly influenza confirmed cases in two countries, namely the USA and China. The outcomes also showed good performances.
Artificial Intelligence in Acute Kidney Injury Risk Prediction
Authors: Gameiro Joana , Branco Tiago , Lopes José António
Published: March 2020

Acute kidney injury (AKI) is a frequent complication in hospitalized patients, which is associated with worse short and long-term outcomes. It is crucial to develop methods to identify patients at risk for AKI and to diagnose subclinical AKI in order to improve patient outcomes. The advances in clinical informatics and the increasing availability of electronic medical records have allowed for the development of artificial intelligence predictive models of risk estimation in AKI. In this review, we discussed the progress of AKI risk prediction from risk scores to electronic alerts to machine learning methods.
Molecular Genetics of Niemann–Pick Type C Disease in Italy: An Update on 105 Patients and Description of 18 NPC1 Novel Variants
Authors: Dardis Andrea , Zampieri Stefania , Gellera Cinzia , Carrozzo Rosalba , Cattarossi Silvia , Peruzzo Paolo , Dariol Rosalia , Sechi Annalisa , Deodato Federica , Caccia Claudio , Verrigni Daniela , Gasperini Serena , Fiumara Agata , Fecarotta Simona , Carecchio Miryam , Filosto Massimiliano , Santoro Lucia , Borroni Barbara , Bordugo Andrea , Brancati Francesco , Russo Cinzia V. , Di Rocco Maja , Toscano Antonio , Scarpa Maurizio , Bembi Bruno
Published: March 2020

Niemann-Pick type C (NPC) disease is an autosomal recessive lysosomal storage disorder caused by mutations in NPC1 or NPC2 genes. In 2009, the molecular characterization of 44 NPC Italian patients has been published. Here, we present an update of the genetic findings in 105 Italian NPC patients belonging to 83 unrelated families (77 NPC1 and 6 NPC2). NPC1 and NPC2 genes were studied following an algorithm recently published. Eighty-four different NPC1 and five NPC2 alleles were identified. Only two NPC1 alleles remained non detected. Sixty-two percent of NPC1 alleles were due to missense variants. The most frequent NPC1 mutation was the p.F284Lfs*26 (5.8% of the alleles). All NPC2 mutations were found in the homozygous state, and all but one was severe. Among newly diagnosed patients, 18 novel NPC1 mutations were identified. The pathogenic nature of 7/9 missense alleles and 3/4 intronic variants was confirmed by filipin staining and NPC1 protein analysis or mRNA expression in patient’s fibroblasts. Taken together, our previous published data and new results provide an overall picture of the molecular characteristics of NPC patients diagnosed so far in Italy.
A Numerical Investigation of the Plunging Phenomenon of Cold Water Discharged from Ocean Thermal Energy Conversion Systems
Authors: Hua Dan , Yasunaga Takeshi , Ikegami Yasuyuki
Published: March 2020

The main role of cold water discharged from ocean thermal energy conversion (OTEC) systems is that deep ocean water, which is dense and nutrient-rich, is released through the condenser and discharged into the ocean surface. We present a numerical simulation in which a cold-water is discharged into a temperature-stratified fluid. A semi-empirical formula relating the outlet flow and the plunging depth was obtained by model analysis, and the k multiplier was 1.15. The model results are consistent with the experimental data.
Wave Energy in Korean Seas from 12-Year Wave Hindcasting
Authors: Eum Ho-Sik , Jeong Weon-Mu , Chang Yeon S. , Oh Sang-Ho , Park Jong-Jip
Published: March 2020

In this study, a numerical simulation is performed to produce wave hindcasting data from 2007 to 2018 for the assessment of wave energy resources in the sea waters of Korea. The hindcasting data are obtained with a relatively fine spatial resolution of 1/20° covering 120–150 °E longitude and 22.4–47.6 °N latitude using the Simulating WAves Nearshore wave model (SWAN). Three different wind fields, those of the European Centre for Medium-Range Weather (ECMWF), National Centers for Environmental Prediction (NCEP), and Japan Meteorological Agency (JMA), are used for the numerical wave simulation. It is observed that the wind field dataset of JMA exhibits the best agreement with available field observation data. For this reason, the wave energy resources are evaluated based on the data hindcasted using the JMA wind field. It is found that the overall magnitudes of wave energy are larger in winter than in summer. The wave energy in August, however, is comparable to the mean wave energy during winter because of the influence of frequent high wave events caused by typhoons. The highest monthly average wave power around Yellow Sea, South Sea, East Sea, and Jeju Island are 13.3, 18.2, 13.7, and 40 kW/m, respectively.
Ship Loading Influence on the Slamming Impact of Typical Sections of an S-175 Container Ship
Authors: He Guanghua , Xie Binyang , Wang Wei , Liu Shuang , Jing Penglin
Published: March 2020

This paper investigates the influence of ship-loading condition on slamming during water entry. Three typical sections of the S-175 container ship, namely the bow, parallel middle body and stern, under three different loading conditions are studied. Full-sized models are established and simulated by commercial software LS-DYNA based on the explicit finite element method (FEM) using the arbitrary Lagrangian–Eulerian (ALE) algorithm. At first, validation is carried out by simulating the Wave Induced Loads on Ships Joint Industry Project II (WILS JIP-II) ship section entering the water and by verifying that the response is in good agreement with published experimental data. Then, nine different cases with three typical sections of the container ship and three different loadings, including the no-load (lightship weight), half-load and full-load weights of the ship, are investigated. Finally, the influence of the ship loading and sectional shape on the water impacts is analyzed and discussed. The present study is useful for the analysis of loading effects on ship slamming at the early stage of ship design.
Prevention Is Better Than the Cure: Risk Management of COVID-19
Authors: McAleer Michael
Published: March 2020

A novel coronavirus was reported to the World Health Organization (WHO) in China on 31 December 2019. The WHO named the disease COVID-19 on 11 February 2020. As of 26 February 2020, the disease has been detected on all continents, except for Antarctica. Daily updates on COVID-19 since early February 2020 have made headline news worldwide for much of 2020. This editorial evaluates risk management based on the Global Health Security (GHS) Index of global health security capabilities in 195 countries. The GHS Index lists the countries best prepared for an epidemic or pandemic. COVID-19 is compared with two related coronavirus epidemics, SARS and MERS, in terms of the number of reported human infections, deaths, countries, major country clusters, timelines, and the likelihood of discovering a safe, effective, and approved vaccine.
The Use of Meta-Surfaces in Vehicular Networks
Authors: Masini Barbara M. , Silva Cristiano M. , Balador Ali
Published: March 2020

Mobility as a service is becoming a new paradigm in the direction of travel planning on the basis of the best service offered by the travelled roads. Hence, the environment in which people move will become smarter and more and more connected to grant services along the whole path. This opens new challenges related not only to the on board connectivity and wireless access technologies, but also on the reliability and efficiency of the surrounding environment. In this context, reconfigurable meta-surfaces play a crucial role, since they can be used to coat buildings, vehicles or any other suitable surfaces and let the environment become an active part of the communication system by opportunistically redirecting (i.e., reflecting, without generating new waves) signals to the target receivers. The objective of this paper is to highlight the limits of current wireless access technologies for vehicular scenarios and to discuss the potential impact of a smart environment made of reconfigurable meta-surfaces on some next generation vehicular use cases, such as cooperative driving and vulnerable road users (VRUs) detection. In addition, a preliminary model is presented to derive, in a simplified way, the performance of an IEEE 802.11p network in terms of collision probability. Even if analytical and based on simplified assumptions, this model has been validated through simulations and allows to compare the performance of the network with and without reconfigurable meta-surfaces.
Lawns in Cities: From a Globalised Urban Green Space Phenomenon to Sustainable Nature-Based Solutions
Authors: Ignatieva Maria , Haase Dagmar , Dushkova Diana , Haase Annegret
Published: March 2020

This opinion paper discusses urban lawns, the most common part of open green spaces and urban green infrastructures. It highlights both the ecosystem services and also disservices provided by urban lawns based on the authors’ experience of working within interdisciplinary research projects on lawns in different cities of Europe (Germany, Sweden and Russia), New Zealand (Christchurch), USA (Syracuse, NY) and Australia (Perth). It complements this experience with a detailed literature review based on the most recent studies of different biophysical, social, planning and design aspects of lawns. We also used an international workshop as an important part of the research methodology. We argue that although lawns of Europe and the United States of America are now relatively well studied, other parts of the world still underestimate the importance of researching lawns as a complex ecological and social phenomenon. One of the core objectives of this paper is to share a paradigm of nature-based solutions in the context of lawns, which can be an important step towards finding resilient sustainable alternatives for urban green spaces in the time of growing urbanisation, increased urban land use competition, various user demands and related societal challenges of the urban environment. We hypothesise that these solutions may be found in urban ecosystems and various local native plant communities that are rich in species and able to withstand harsh conditions such as heavy trampling and droughts. To support the theoretical hypothesis of the relevance of nature-based solutions for lawns we also suggest and discuss the concept of two natures—different approaches to the vision of urban nature, including the understanding and appreciation of lawns. This will help to increase the awareness of existing local ecological approaches as well as an importance of introducing innovative landscape architecture practices. This article suggests that there is a potential for future transdisciplinary international research that might aid our understanding of lawns in different climatic and socio-cultural conditions as well as develop locally adapted (to environmental conditions, social needs and management policies) and accepted nature-based solutions.
Investigation of Galling Wear Using Acoustic Emission Frequency Characteristics
Authors: Shanbhag Vignesh. V. , Rolfe Bernard. F. , Pereira Michael. P.
Published: March 2020

In the sheet metal stamping process, during sliding contact between the tool and sheet, it is expected that severe events such as tool wear or fracture on the sheet generate acoustic emission (AE) burst waveforms. Attempts have been made in the literature to correlate the AE burst waveform with the wear mechanisms. However, there is a need for additional studies to understand the frequency characteristics of the AE burst waveform due to the severity and progression of the galling wear. This paper will determine the AE frequency characteristics that can be used to monitor galling wear, independent of the experimental process examined. The AE burst waveforms generated during the stamping and scratch tests are analysed in this paper to understand the change in the AE frequency characteristics with the galling severity. These AE burst waveforms were investigated using the Hilbert Huang Transform (HHT) time-frequency technique, band power, and mean-frequency. Subsequently, these AE frequency features are correlated with the wear behaviour observed via high-resolution profilometer images of the stamped parts and scratch surfaces. Initially, the HHT technique is applied to the AE burst waveform to understand the influence of wear severity in the power distribution over the wide AE frequency range. Later, the AE bandpower feature is used to quantitatively analyse the power in each frequency interval during the unworn and worn tool conditions. Finally, the mean-frequency of AE signal is identified to be able to determine the onset of galling wear. The new knowledge defined in this paper is the AE frequency features and wear measurement feature that can be used to indicate the onset of galling wear, irrespective of the processes examined.
Maintenance of Alveolar Ridge Dimensions Utilizing an Extracted Tooth Dentin Particulate Autograft and Platelet-Rich fibrin: A Retrospective Radiographic Cone-Beam Computed Tomography Study
Authors: Pohl Snjezana , Binderman Itzhak , Tomac Jelena
Published: March 2020

This study utilized radiographic comparative analysis in order to evaluate dimensional ridge changes four months after tooth extraction and immediate grafting with mineralized dentin particulate autograft and chopped platelet-rich fibrin. Fifty-eight extraction sockets with up to 2 mm of missing buccal bone in the coronal aspect compared to the lingual bone were included. Graft material was covered with either a platelet-rich fibrin membrane or collagen sponge with no effort to achieve primary closure. The dimensional changes of the ridge were assessed on cone-beam computed tomography (CBCT) images acquired prior to extraction and four months later. The reduction in the buccal bone plate thickness 1 mm, 3 mm, and 5 mm below the buccal crest was −0.87 ± 0.84 mm, −0.60 ± 0.70 mm, and −0.41 ± 0.55 mm, respectively. The mean ridge width changes 1 mm, 3 mm, and 5 mm below the crest were −1.38 ± 1.24 mm, −0.82 ± 1.13 mm, and −0.43 ± 0.89 mm, respectively. The average mid-buccal bone height gain was +1.1%, while the mid-lingual height gain was 5.6%. A mineralized dentin autograft with platelet-rich fibrin is effective in preserving post-extraction alveolar ridge dimensions.
Design and Mechanical Characterization of Voronoi Structures Manufactured by Indirect Additive Manufacturing
Authors: Almonti Daniele , Baiocco Gabriele , Tagliaferri Vincenzo , Ucciardello Nadia
Published: March 2020

Additive manufacturing (AM) is a production process for the fabrication of three-dimensional items characterized by complex geometries. Several technologies employ a localized melting of metal dust through the application of focused energy sources, such as lasers or electron beams, on a powder bed. Despite the high potential of AM, numerous burdens afflict this production technology; for example, the few materials available, thermal stress due to the focused thermal source, low surface finishing, anisotropic properties, and the high cost of raw materials and the manufacturing process. In this paper, the combination by AM of meltable resins with metal casting for an indirect additive manufacturing (I-AM) is proposed. The process is applied to the production of open cells metal foams, similar in shape to the products available in commerce. However, their cellular structure features were designed and optimized by graphical editor Grasshopper®. The metal foams produced by AM were cast with a lost wax process and compared with commercial metal foams by means of compression tests.
Ion-Locking in Solid Polymer Electrolytes for Reconfigurable Gateless Lateral Graphene p-n Junctions
Authors: Liang Jierui , Xu Ke , Arora Swati , Laaser Jennifer E. , Fullerton-Shirey Susan K.
Published: March 2020

A gateless lateral p-n junction with reconfigurability is demonstrated on graphene by ion-locking using solid polymer electrolytes. Ions in the electrolytes are used to configure electric-double-layers (EDLs) that induce p- and n-type regions in graphene. These EDLs are locked in place by two different electrolytes with distinct mechanisms: (1) a polyethylene oxide (PEO)-based electrolyte, PEO:CsClO4, is locked by thermal quenching (i.e., operating temperature < Tg (glass transition temperature)), and (2) a custom-synthesized, doubly-polymerizable ionic liquid (DPIL) is locked by thermally triggered polymerization that enables room temperature operation. Both approaches are gateless because only the source/drain terminals are required to create the junction, and both show two current minima in the backgated transfer measurements, which is a signature of a graphene p-n junction. The PEO:CsClO4 gated p-n junction is reconfigured to n-p by resetting the device at room temperature, reprogramming, and cooling to T < Tg. These results show an alternate approach to locking EDLs on 2D devices and suggest a path forward to reconfigurable, gateless lateral p-n junctions with potential applications in polymorphic logic circuits.
Investigation on Ultrasonic Welding Attributes of Novel Carbon/Elium® Composites
Authors: Bhudolia Somen K. , Gohel Goram , Leong Kah Fai , Barsotti Robert J.
Published: March 2020

Joining large and complex polymer–matrix composite structures is becoming increasingly important in industries such as automobiles, aerospace, sports, wind turbines, and others. Ultrasonic welding is an ultra-fast joining process and also provides excellent joint quality as a cost-effective alternative to other joining processes. This research aims at investigating the welding characteristics of novel methyl methacrylate Elium®, a liquid thermoplastic resin. Elium® is the first of its kind of thermoplastic resin, which is curable at room temperature and is suitable for mass production processes. The welding characteristics of Elium® composites were investigated by optimizing the welding parameters with specially designed integrated energy directors (ED) and manufactured using the Resin transfer molding process. The results showed a 23% higher lap shear strength for ultrasonically welded composite joints when compared to the adhesively bonded joints. The optimized welding time for the ultrasonic welded joint was found to be 1.5 s whereas it was 10 min for the adhesively bonded joint. Fractographic analysis showed the significant plastic deformation and shear cusps formation on the fractured surface, which are typical characteristics for strong interfacial bonding.