bluuebunny/crossref_metadata_2025 huggingface dataset:
SFPQ Promotes Lung Cancer Malignancy via Regulation of CD44 v6 Expression
Authors: Yang Libang , Yang Jianbo , Jacobson Blake , Gilbertsen Adam , Smith Karen , Higgins LeeAnn , Guerrero Candace , Xia Hong , Henke Craig A. , Lin Jizhen
Published: May 2022

Mesenchymal stem cells (MSCs) contribute to tumor pathogenesis and elicit antitumor immune responses in tumor microenvironments. Nuclear proteins might be the main players in these processes. In the current study, combining spatial proteomics with ingenuity pathway analysis (IPA) in lung non-small cell (NSC) cancer MSCs, we identify a key nuclear protein regulator, SFPQ (Splicing Factor Proline and Glutamine Rich), which is overexpressed in lung cancer MSCs and functions to promote MSCs proliferation, chemical resistance, and invasion. Mechanistically, the knockdown of SFPQ reduces CD44v6 expression to inhibit lung cancer MSCs stemness, proliferation in vitro, and metastasis in vivo. The data indicates that SFPQ may be a potential therapeutic target for limiting growth, chemotherapy resistance, and metastasis of lung cancer.
General Anesthesia for Patients With Chronic Obstructive Pulmonary Disease and Postoperative Respiratory Failure: A Retrospective Analysis of 120 Patients
Authors: Hou Ruixue , Miao Fangfang , Jin Di , Duan Qingfang , Yin Cheng , Feng Qunpeng , Wang Tianlong
Published: May 2022

Background: Chronic obstructive pulmonary disease (COPD) has been considered a risk factor for postoperative respiratory failure after general anesthesia. However, the association between COPD severity and postoperative respiratory failure among COPD patients is unknown. Our aim was to compare the prevalence of postoperative respiratory failure in COPD patients according to disease severity after general anesthesia.

Methods: We retrospectively reviewed COPD patients undergoing spinal surgery with general anesthesia at our clinical center between January 2016 and January 2021. These subjects were divided into four groups (group I = mild COPD, group II = moderate COPD, group III = severe COPD, and group IV = very severe COPD) according to their preoperative lung function. The primary endpoint was a respiratory failure 1 week after surgery. The diagnosis of respiratory failure was made with the presence of one or more of the following criteria: prolonged ventilator dependence, unplanned postoperative intubation, and partial pressure of arterial oxygen (PaO2) ≤ 50 mmHg while the patient was breathing ambient air in the hospital. The extubation time, perioperative PaO2 and partial pressure of arterial carbon dioxide (PaCO2), postoperative lung infection, and length of hospitalization were also compared.

Results: A total of 120 patients who underwent spinal surgery with general anesthesia were included in this retrospective study. Postoperative respiratory failure occurred in 0 (0.0%) patient in group I, 1 (1.5%) patient in group II, 1 (2.5%) patient in group III, and 1 (14.5%) patient in group IV 1 week after surgery (p = 0.219). The duration of anesthesia was 243.3 ± 104.3 min in group I, 235.5 ± 78.8 min in group II, 196.0 ± 66.3 min in group III, and 173.1 ± 63.7 min in group IV (p < 0.001). Preoperative PaO2, PaCO2, intraoperative oxygenation index [a ratio of PaO2 to fraction of inspired oxygen (FiO2)], and postoperative PaO2 were significantly different among the four groups (p < 0.001, 0.001, 0.046, <0.001, respectively). No significant differences among the four groups were seen in extubation time, pulmonary infection, or hospital stay (p = 0.174, 0.843, 0.253, respectively). The univariate analysis revealed that higher preoperative PaO2 was associated with a lower rate of postoperative respiratory failure (OR 0.83; 95% CI, 0.72 to 0.95; p = 0.007).

Conclusion: The severity of COPD as assessed with GOLD classification was not associated with the development of postoperative respiratory failure. However, lower preoperative PaO2 was associated with greater odds of postoperative respiratory failure in COPD patients.
Automatic Detection and Counting of Wheat Spikelet Using Semi-Automatic Labeling and Deep Learning
Authors: Qiu Ruicheng , He Yong , Zhang Man
Published: May 2022

The number of wheat spikelets is an important phenotypic trait and can be used to assess the grain yield of the wheat crop. However, manual counting of spikelets is time-consuming and labor-intensive. To develop a cost-effective and highly efficient phenotyping system for counting the number of spikelets under laboratory conditions, methods based on imaging processing techniques and deep learning were proposed to accurately detect and count spikelets from color images of wheat spikes captured at the grain filling stage. An unsupervised learning-based method was first developed to automatically detect and label spikelets from spike color images and build the datasets for the model training. Based on the constructed datasets, a deep convolutional neural network model was retrained using transfer learning to detect the spikelets. Testing results showed that the root mean squared errors, relative root mean squared errors, and the coefficients of determination between the automatic and manual counted spikelets for four wheat lines were 0.62, 0.58, 0.54, and 0.77; 3.96, 3.73, 3.34, and 4.94%; and 0.73, 0.78, 0.84, and 0.67, respectively. We demonstrated that the proposed methods can effectively estimate the number of wheat spikelets, which improves the counting efficiency of wheat spikelets and contributes to the analysis of the developmental characteristics of wheat spikes.
Who Denigrates Today’s Youth?: The Role of Age, Implicit Theories, and Sharing the Same Negative Trait
Authors: Protzko John , Schooler Jonathan W.
Published: May 2022

Adults perceive the youth of the present as being worse than from when they were young. This phenomenon has been shown to be a product of a memory bias, adults are unable to accurately recall what children were like in the past so they impose their current selves onto their memories. In two studies using American adults (N = 2,764), we seek to connect this finding to age, implicit theories of change, and extend the beliefs in the decline of the youth to new domains. Here we show as people age, they hold harsher beliefs about present children. Those who believe a trait does not change throughout the lifespan exhibit more forgiving attitudes toward the youth of today, believing they may not be in such decline on that trait. Finally, people who are low in a negative trait believe strongly that children are becoming more deficient in that particular trait (e.g., those who are not narcissistic believe the youth are becoming more narcissistic).
Profiles of Achievement Motivation and Performance in Middle School: Links to Student Background and Perceived Classroom Climate
Authors: Erentaitė Rasa , Vosylis Rimantas , Sevalneva Daiva , Melnikė Eglė , Raižienė Saulė , Daukantaitė Daiva
Published: May 2022

Achievement motivation and performance at school are reciprocally related, however, empirical studies report a large variability of findings and, in some cases, weaker than expected associations between these constructs. To further our understanding of the motivation–performance link, we examined typical patterns of motivation and performance and their correlates, in two cohorts of 8th-grade students (N1 = 998, N2 = 441). As expected, we identified both concordant and discordant patterns of achievement motivation and performance. In two subgroups, specifically, those characterized by low motivation and low performance (34% of the sample) and those characterized by high motivation and high performance (18% of the sample), the levels of motivation were highly concordant with scores on math and reading tests. In contrast, the other two profiles—weak motivation with elevated performance (38% of all sample) and high motivation with low performance (9% of the sample) had divergent patterns of motivation and performance. The subgroups also differed on student socio-economic background, special educational needs, gender, as well as perceptions of classroom climate. Overall, our findings reveal context-dependent patterns of the relationship between aspects of achievement motivation and performance.
Does Self-Sacrifice Make Me Great? Research on the Relationship Between Employee Conscientiousness and Pro-Social Rule Breaking
Authors: Liu Xiayi , Wang Hongqing , Liu Xiajun
Published: May 2022

Based on the theory of purposeful work behavior, this study proposed that the two facets of employee conscientiousness, namely duty orientation and achievement orientation, have opposite effects on pro-social rule breaking (PSRB). We also explored the moderating effect of employees’ task characteristic (job autonomy) and social characteristic (leader reward omission) on the above relationships. Using two-wave data collected from 216 employee-supervisor dyads, we found that duty orientation was positively related to PSRB, while achievement orientation was negatively related to PSRB. Further, job autonomy, by amplifying employees’ perceived meaningfulness of their higher-order implicit goals, can strengthen the positive effect of duty orientation and the negative effect of achievement orientation on PSRB. Similarly, leader reward omission could also activate the negative effect of achievement orientation and PSRB, but not significantly moderate the positive relationship between duty orientation and PSRB. By separating the distinct role of facet-specific personality, our study sheds light on the relationship between employee conscientiousness and PSRB.
Mechanisms of Participation in Vocational Education and Training in Europe
Authors: Milmeister Paul , Rastoder Merlin , Houssemand Claude
Published: May 2022

This article aims to analyze vocational education and training in Europe and to model mechanisms of educational and vocational choice. First, we expose the differences between VET approaches in Europe. Secondly, a sociological analysis is provided. When VET systems were first created, aspects such as work culture or diverging political concerns led to different responses in the various countries. Thirdly, we present a psychological approach of the educational and vocational choice which draws on a process where profession images are compared with one’s own self-image. Finally, we present an integrated explanatory model of the vocational choice, based on sociological and psychological dimensions. In conclusion, we propose several plans of action in order to support and inform students regarding educational choice and to improve valorization of the VET track.
Self-Control Mediates, and Mobile Phone Dependence Moderates, the Relationship Between Psychological Capital and Attitudes Toward Physical Exercise Among Chinese University Students
Authors: Liu Min , Li Xinnan , He Zhonghui
Published: May 2022

Physical exercise can improve the psychological capital while the attitude toward physical exercise will affect one’s exercise behavior. However, moderating factors that may influence how physical exercise affects psychological capital remains unknown. We conducted a survey of 519 Chinese university students to investigate the mediating role of self-control between attitudes toward physical exercise and psychological capital, and whether this mediating role is moderated by mobile phone dependence. We found that attitudes toward physical exercise had a positive predictive effect on the psychological capital of university students. Besides, self-control mediated the relationship between attitudes toward physical exercise and psychological capital. The influence of self-control on psychological capital was moderated by mobile phone dependence: the influence of self-control on psychological capital decreased with higher mobile phone dependence. Our results suggest that attitudes toward physical exercise can positively predict the psychological capital of university students, with self-control playing a mediating role between them.
Pleistocene Hypothesis – Moving Savanna Perceptual Preference Hypothesis Beyond Savanna
Authors: Rathmann Joachim , Korpela Kalevi M. , Stojakowits Philipp
Published: May 2022

We provide an extension of the Savanna perceptual preference hypothesis (“Savanna Hypothesis”), supposing that interaction with landscapes offering survival advantage for human groups during evolution might have gradually evolved to permanent landscape preferences. This additional support is based on the palaeoenvironmental analysis of the spread of modern humans into Europe in the late Pleistocene and their living environments there. Our hypothesis is that the preference for park-like landscapes after African savannas experienced a kind of “refreshment” in the Pleistocene. Thus, preferences for certain types of natural settings and scenes may have a more continuous evolutionary history than previously thought. The extended Savanna Hypothesis termed “Pleistocene Hypothesis” might stimulate further work on this important topic linking human evolution and human environmental preferences.
Brazilian Food Handlers' Years of Work in the Foodservice and Excess Weight: A Nationwide Cross-Sectional Study
Authors: Fideles Ingrid C. , Akutsu Rita de Cássia C. de A. , Costa Priscila R. de F. , Souza Jamacy C. , Barroso Rosemary da R. F. , Botelho Raquel B. A. , Han Heesup , Raposo António , Ariza-Montes Antonio , Vega-Muñoz Alejandro , Zandonadi Renata P.
Published: May 2022

This study aimed to evaluate the association between the years of work of food handlers in the foodservice and excess weight among Brazilian low-income food handlers. A total of 559 food handlers from all Brazilian regions were characterized using a questionnaire. Weight and height were measured to estimate the Body Mass Index and classify the individuals. The association between food handlers' years of work in the foodservice, anthropometric status, and other variables (gender, age group, educational level, participation in a government program and per capita income at home and energetic consumption) were performed using Pearson's chi-square test (p < 0.05). Multinomial logistic regression analyses were performed (p < 0.05) as well as sensitivity tests using the outcome continuously and transformed, excluding underweight individuals, in a multivariate linear regression model. Most of the sample was female (63.1%), aged between 21 and 40 years old (63.5%), and 53.3% had studied up to complete elementary school. Almost 41% of the food handlers had less than half the minimum wage per capita income. Of the evaluated individuals, 59.9% presented excess weight. There was an association with family per capita income (Odds Ratio - OR: 1.73; Confidence interval - CI95%: 1.09–2.75); handlers whose per capita income was ≤0.5 minimum wage had a 73% higher chance of obesity than those with higher income. Working in foodservive ≥3 years increased the chance of being overweight by 96% compared to those who work for <3 years (OR: 1.96; CI95%: 1.11–3.49). No significant association was found between the years of work of food handlers in the foodservice and obesity. Since work-related factors may contribute to the high prevalence of excess weight, including working in a food handling environment, the government and employers should consider workplace interventions. These would guide the food handlers in avoiding high rates of excess weight and their consequences on public health. Excess weight is an important driver of costs in the workplace associated with absenteeism, job change, and diseases. More studies are necessary to clarify the relationship between the factors related to work and the anthropometric status of food handlers since excess weight is multifactorial.
Improving Public Health and Governance in COVID-19 Response: A Strategic Public Procurement Perspective
Authors: Yan Ran , Cao Fuguo
Published: May 2022

Good governance is the basis of dealing with major emergencies and protecting public health. It has become a major issue of the central government to construct a scientific procurement and supply system of emergency supplies. This article constructs the analytical framework of strategic procurement and expounds the realization mechanism of strategic procurement under an emergency situation to reflect China's procurement practice in COVID-19 response and improve public health and governance. Using case study, semi-structured interviews, and the Nvivo text analysis, this study found that emphasizing the strategic function of securing the public health,the top status of MSG, cross-sector procurement team, strong procurement and supply integration, comprehensive and in-depth procurement synergy mechanism are the successful experiences of China's emergency procurement. However, due to the temporary nature of the emergency procurement mechanism, strategic procurement planning, procurement management specialization, and procurement supply integration still need to be improved. The findings of this study further suggest that to improve public health and governance, it is pivotal to reconstruct the government procurement law to make it compatible with the emergency procurement and transform the government procurement system into a strategic procurement in a consistent and coherent way.
Myosteatosis as a Shared Biomarker for Sarcopenia and Cachexia Using MRI and Ultrasound
Authors: Lortie Jevin , Rush Benjamin , Osterbauer Katie , Colgan T. J. , Tamada Daiki , Garlapati Sujay , Campbell Toby C. , Traynor Anne , Leal Ticiana , Patel Viharkumar , Helgager Jeffrey J. , Lee Kenneth , Reeder Scott B. , Kuchnia Adam J.
Published: May 2022

Purpose

Establish bedside biomarkers of myosteatosis for sarcopenia and cachexia. We compared ultrasound biomarkers against MRI-based percent fat, histology, and CT-based muscle density among healthy adults and adults undergoing treatment for lung cancer.

Methods

We compared ultrasound and MRI myosteatosis measures among young healthy, older healthy, and older adults with non-small cell lung cancer undergoing systemic treatment, all without significant medical concerns, in a cross-sectional pilot study. We assessed each participant's rectus femoris ultrasound-based echo intensity (EI), shear wave elastography-based shear wave speed, and MRI-based proton density fat-fraction (PDFF). We also assessed BMI, rectus femoris thickness and cross-sectional area. Rectus femoris biopsies were taken for all older adults (n = 20) and we analyzed chest CT scans for older adults undergoing treatment (n = 10). We determined associations between muscle assessments and BMI, and compared these assessments between groups.

Results

A total of 10 young healthy adults, 10 older healthy adults, and 10 older adults undergoing treatment were recruited. PDFF was lower in young adults than in older healthy adults and older adults undergoing treatment (0.3 vs. 2.8 vs. 2.9%, respectively, p = 0.01). Young adults had significantly lower EI than older healthy adults, but not older adults undergoing treatment (48.6 vs. 81.8 vs. 75.4, p = 0.02). When comparing associations between measures, PDFF was strongly associated with EI (ρ = 0.75, p < 0.01) and moderately negatively associated with shear wave speed (ρ = −0.49, p < 0.01) but not BMI, whole leg cross-sectional area, or rectus femoris cross-sectional area. Among participants with CT scans, paraspinal muscle density was significantly associated with PDFF (ρ = −0.70, p = 0.023). Histological markers of inflammation or degradation did not differ between older adult groups.

Conclusion

PDFF was sensitive to myosteatosis between young adults and both older adult groups. EI was less sensitive to myosteatosis between groups, yet EI was strongly associated with PDFF unlike BMI, which is typically used in cachexia diagnosis. Our results suggest that ultrasound measures may serve to determine myosteatosis at the bedside and are more useful diagnostically than traditional weight assessments like BMI. These results show promise of using EI, shear wave speed, and PDFF proxies of myosteatosis as diagnostic and therapeutic biomarkers of sarcopenia and cachexia.
Non-pharmacological Management of Non-productive Chronic Cough in Adults: A Systematic Review
Authors: Ilicic Ana Maria , Oliveira Ana , Habash Razanne , Kang Yejin , Kho Michelle , Goldstein Roger , Brooks Dina
Published: May 2022

Background

Chronic cough is a common reason for medical referral and its prevalence is on the rise. With only one pharmaceutical therapy currently under review for the treatment of refractory chronic cough, exploring non-pharmacological chronic cough management therapies is important. This systematic review summarizes the effectiveness of non-pharmacological chronic cough therapies in adults with non-productive refractory chronic cough or cough due to chronic respiratory diseases.

Methods

We searched Medline, Embase, Cochrane, CINAHL, and Scopus from inception to September 2021. Randomized controlled trials published in English, Portuguese, or French, and examining the effects of non-pharmacological therapies in adults with chronic non-productive cough (>8 weeks; <2 teaspoons sputum) were included. Mean differences, medians, and odds ratios were calculated as appropriate.

Results

16,546 articles were identified and six articles representing five unique studies were included. Studies evaluated 228 individuals with refractory chronic cough or chronic cough due to a chronic respiratory disease [162 women (71%); 52 ± 11 to 61 ± 8 years old]. Obstructive sleep apnea was the only chronic respiratory disease studied. Non-pharmacological therapies included education, cough suppression, breathing techniques, mindfulness, and continuous positive airway pressure. When standing alone, non-pharmacological cough therapies improved cough-specific health related quality of life when not associated with interventions (mean diff MD 1.53 to 4.54), cough frequency (MD 0.59 95%CI 0.36 to 0.95), and voice outcomes (MD 0.3 to 1) when compared to control interventions.

Conclusion

The evidence of non-pharmacological therapies for non-productive chronic cough is limited. Existing studies reflect the heterogeneity in study design, sample size, and outcome measures. Thus, clinical recommendations for using the most effective interventions remain to be confirmed.
Cracking The Mystery Of Crater Lake’s Unique Newts
Authors: Ray Andrew M. , Spear Stephen F. , Girdner Scott F. , Hering David K.
Published: May 2022

Why does Earth have such an incredible variety of plants and animals? One little newt helps tell the story. In Crater Lake, the world’s clearest lake, a uniquely colored newt was discovered. Early explorers named it the Mazama newt. For more than 100 years, the newt has been considered a subspecies of the more common rough-skinned newt. A “subspecies” refers to a collection of animals or plants that can interbreed but can often be separated from other individuals of the same species by appearance. Nobody knew exactly how unique the Mazama newt was until scientists took another look. Using a mix of clues, like DNA, skin color, and even poison levels, scientists are cracking the mystery of how the Mazama newt differs from its neighbors. Learning about the newt’s unique characteristics and about other members of the Crater Lake ecosystem is important for protecting biodiversity and lake health.
The (Un)real Existence of ADHD—Criteria, Functions, and Forms of the Diagnostic Entity
Authors: Honkasilta Juho , Koutsoklenis Athanasios
Published: May 2022

The contemporary conceptualization of Attention Deficit Hyperactivity Disorder (ADHD) as a complex, multifactorial neurodevelopmental disorder cannot be understood as such without a complex assemblage of political, economic, and cultural processes that deem the conceptualization to be valuable and useful. In this article we use the notion of psychiatrization as a lens through which to see parts of these processes that make up ADHD what it is. In the first part of the article, we critically assess the scientific basis of the ADHD diagnosisviaexamining its diagnostic criteria as presented in the current fifth edition of Diagnostic and Statistical Manual of Mental Disorders (DSM), the so called “Bible” of modern psychiatry. The second part of the article asks what is done with the ADHD diagnostic entity and with the idea that it represents a natural neurodevelopmental state within an individual—something an individual has—as represented in the DSM-5. Drawn from our previous research, we analyze how ADHD becomes real in discourse practice as a powerful semiotic mediator through analysis of the various functions and forms in which it takes shape in institutional, social, and individual levels. We conclude that the frequent changes in the diagnostic criteria of ADHD do not reflect any real scientific progress. Among other reasons, they change to match better the maneuvers of individuals when navigating an increasingly psychiatrized society in the search for recognition, support, category membership, immunity, sympathy, and sense of belonging.
Systematic Review: Heat Treatments on Phenolic Content, Antioxidant Activity, and Sensory Quality of Malaysian Mushroom: Oyster (Pleurotus spp.) and Black Jelly (Auricularia spp.)
Authors: Izham Inshirah , Avin Farhat , Raseetha Siva
Published: May 2022

Pleurotus spp. and Auricularia spp. are popular species consumed by the Malaysian community. Recently, due to increased awareness, both mushrooms are also being consumed for their bioactive compounds, ergothioneine, and antioxidant properties and has been used since earlier ages as therapeutic remedies. The bioactive compounds such as phenol, flavonoid and ergothioneine found in both Pleurotus and Auricularia mushrooms were explored. Differences in heat treatments (microwave, hot air drying, and solar drying) and cooking methods may affect the content of bioactive compounds and their properties. Similarly, sensory acceptance by consumers may be affected too. Antioxidant properties using DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical and FRAP (ferric reducing antioxidant power) assay of both raw and heat-treated mushrooms are included. Microwave drying retained color characteristics and bioactive compounds in both mushrooms. To add value to this review, a survey on the consumption pattern of Pleurotus and Auricularia species among Malaysians has been conducted online and concluded that Pleurotus species is the most considered species compared to Auricularia mushroom and almost half of the respondents were not aware that heat may deplete nutritional contents in mushroom despite agreeing both gave beneficial health in diets.
Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides
Authors: Li Dong-Yan , Cui Xing-Yang , Huang Xin-Yi , Hu Yue , Tian Xiao-Xiao , Wang Tao , Yang Yong-Bo , Wang Qian , Tian Zhi-Jun , Cai Xue-Hui , An Tong-Qing
Published: May 2022

Porcine reproductive and respiratory syndrome (PRRS) is a widespread disease with great economic importance in the pig industry. Although vaccines against the PRRS virus (PRRSV) have been employed for more than 20 years, differentiating infected from vaccinated animals remains challenging. In this study, all 907 non-structural protein 2 (NSP2) full-length sequences of PRRSV-2 available from GenBank were aligned. Two peptides, at positions 562–627 (m1B) and 749–813 (m2B) of NSP2, were selected, and their potential for use in differential diagnosis was assessed. Both m1B and m2B were recognized by PRRSV-positive pig serum in peptide-coated enzyme-linked immunosorbent assays. Further epitope identification yielded five overlapping short peptides for the immunodominant regions of m1B and m2B. Using the infectious clone of PRRSV HuN4-F112 as a template, the deletion mutants, rHuN4-F112-m1B, rHuN4-F112-m2B, and rHuN4-F112-C5-m1B-m2B, were generated and successfully rescued in Marc-145 cells. Growth kinetics revealed that the deletion of m1B and m2B did not significantly affect virus replication. Hence, m1B and m2B show potential as molecular markers for developing a PRRSV vaccine.
Use of Non-Steroidal Anti-Inflammatory Drugs and Attitudes to Pain in Pasture-Based Dairy Cows: A Comparative Study of Farmers and Veterinarians
Authors: Browne Natasha , Conneely Muireann , Hudson Chris
Published: May 2022

Pain is a significant welfare concern within the dairy industry. Recognizing and managing pain are important factors for safeguarding animal welfare. A questionnaire was sent via post to Irish dairy farmers and large animal veterinarians to assess attitudes to pain and the use of non-steroidal anti-inflammatory drugs (NSAIDs) in pasture-based dairy cows. The questionnaire could also be completed online. A total of 1,002 surveys were received from dairy farmers and 116 from livestock veterinarians. Veterinarians and farmers generally perceived the same conditions and procedures as the most painful. However, farmers scored surgical procedures significantly higher than veterinarians, and veterinarians scored lameness-related conditions, mastitis (clots in milk only) and hock hair loss significantly higher than farmers. Higher pain scores for conditions and procedures given by dairy farmers and veterinarians were associated with increased NSAID use. However, the use of NSAIDs was low, relative to the pain score, for Burdizzo castration (farmers and veterinarians), white line separation (farmers and veterinarians) and abscess (veterinarians), mastitis with clots in milk only (farmers) and calving with no assistance (farmers). Veterinarians who graduated less recently had significantly lower odds of using NSAIDs, and farmers that completed the survey online, had a larger herd size, completed education up to level four or five (as opposed to level three) and those who seemed to have less knowledge on analgesics, had significantly lower odds of using NSAIDs. Empathy was not found to be associated with NSAID use and no correlation was found between pain and empathy scores. Veterinarians perceived cost as more of a barrier than farmers did; therefore, NSAIDs should be offered more readily. For those working with dairy cows, there is a need to continue education on the benefits of analgesia, especially for conditions and procedures that have low NSAID use relative to pain score. The habituation of humans to pain in animals needs to be prevented to enable pain to be recognized and managed appropriately. Pain scores can be used as a benchmark for veterinarians and farmers to determine how their perception of pain compares to others, and see how this may influence their NSAID use.
How Vaccination Rumours Spread Online: Tracing the Dissemination of Information Regarding Adverse Events of COVID-19 Vaccines
Authors: Harper Tauel , Attwell Katie
Published: May 2022

Objectives: To trace the emergence and dissemination of the most prominent rumours about potential adverse effects of COVID-19 vaccines.

Methods: We use a weekly Google Trends search to gather information about what alleged adverse events are being associated with COVID vaccines by the general population. We then use CrowdTangle and Factiva searches to examine how discussions about the five most prominent adverse events have spread through traditional media channels and Facebook.

Results: Traditional mass media reporting remains crucial in both promoting and moderating discussions around alleged adverse events. While some cases illustrate that social media networks can synthesise and amplify rumours about adverse events, traditional media coverage remains crucial as a forum for exploring and debunking spurious claims.

Conclusion: Traditional media stories still bear signficant responsibility as credibility markers for rumours about vaccine adverse events. Journalists should therefore be encouraged to be particularly earnest when reporting such stories, and the scientific community should aid journalists in this task by clearly responding to any rumours emerging online.
Progression Patterns in Non-Contrast-Enhancing Gliomas Support Brain Tumor Responsiveness to Surgical Lesions
Authors: Brenner Steffen , Hartzendorf Sebastian , Vogt Philip , Maier Elena , Etminan Nima , Jung Erik , Wick Wolfgang , Sahm Felix , Winkler Frank , Ratliff Miriam
Published: May 2022

Purpose: The overall benefit of surgical treatments for patients with glioma is undisputed. We have shown preclinically that brain tumor cells form a network that is capable of detecting damage to the tumor, and repair itself. The aim of this study was to determine whether a similar mechanism might contribute to local recurrence in the clinical setting.

Methods: We evaluated tumor progression patterns of 24 initially non-contrast-enhancing gliomas that were partially resected or biopsied. We measured the distance between the new contrast enhancement developing over time, and prior surgical lesioning, and evaluated tumor network changes in response to sequential resections by quantifying tumor cells and tumor networks with specific stainings against IDH1-R132H.

Results: We found that new contrast enhancement appeared within the residual, non-enhancing tumor mass in 21/24 patients (87.5%). The location of new contrast enhancement within the residual tumor region was non-random; it occurred adjacent to the wall of the resection cavity in 12/21 patients (57.1%). Interestingly, the density of the glioma cell network increased in all patient tumors between initial resection or biopsy and recurrence. In line with the histological and radiological malignization, Ki67 expression increased from initial to final resections in 14/17 cases.

Conclusion: The non-random distribution of glioma malignization in patients and unidirectional increase of anatomical tumor networks after surgical procedures provides evidence that surgical lesions, in the presence of residual tumor cells, can stimulate local tumor progression and tumor cell network formation. This argues for the development of intraoperative treatments increasing the benefits from surgical resection by specifically disrupting the mechanisms of local recurrence, particularly tumor cell network functionality.
Pretransplant and Posttransplant Alcohol Consumption and Outcomes in Kidney Transplantation: A Prospective Multicenter Cohort Study
Authors: Jung Hee-Yeon , Jeon Yena , Huh Kyu Ha , Park Jae Berm , Kim Myung-Gyu , Lee Sik , Han Seungyeup , Ro Han , Yang Jaeseok , Ahn Curie , Cho Jang-Hee , Park Sun-Hee , Kim Yong-Lim , Kim Chan-Duck
Published: May 2022

The impact of pretransplant and posttransplant alcohol consumption on outcomes in kidney transplant recipients (KTRs) is uncertain. Self-reported alcohol consumption was obtained at the time of transplant and 2 years after transplant in a prospective cohort study. Among 907 KTRs, 368 (40.6%) were drinkers at the time of transplant. Compared to non-drinkers, alcohol consumption did not affect the risk of death-censored graft failure (DCGF), biopsy-proven acute rejection (BPAR), cardiovascular events, or all-cause mortality. Compared to persistent non-drinkers, the development of DCGF, BPAR, cardiovascular events, all-cause mortality, or posttransplant diabetes mellitus was not affected by the alcohol consumption pattern (persistent, de novo, or stopped drinking) over time. However, de novo drinkers had a significantly higher total cholesterol (p < 0.001) and low-density lipoprotein cholesterol levels (p = 0.005) compared to persistent non-drinkers 5 years after transplant, and had significantly higher total cholesterol levels (p = 0.002) compared to the stopped drinking group 7 years after transplant, even after adjusting for the use of lipid-lowering agents, age, sex, and body mass index. Although pretransplant and posttransplant alcohol consumption were not associated with major outcomes in KTRs during the median follow-up of 6.0 years, a new start of alcohol use after KT results in a relatively poor lipid profile.

Clinical Trial Registration:clinicaltrials.gov, identifier NCT02042963.
Biliary Viability Assessment and Treatment Options of Biliary Injury During Normothermic Liver Perfusion—A Systematic Review
Authors: Dingfelder Jule , Rauter Laurin , Berlakovich Gabriela A. , Kollmann Dagmar
Published: May 2022

In recent years, significant progress has been made in the field of liver machine perfusion. Many large transplant centers have implemented machine perfusion strategies in their clinical routine. Normothermic machine perfusion (NMP) is primarily used to determine the quality of extended criteria donor (ECD) organs and for logistical reasons. The vast majority of studies, which assessed the viability of perfused grafts, focused on hepatocellular injury. However, biliary complications are still a leading cause of post-transplant morbidity and the need for re-transplantation. To evaluate the extent of biliary injury during NMP, reliable criteria that consider cholangiocellular damage are needed. In this review, different approaches to assess damage to the biliary tree and the current literature on the possible effects of NMP on the biliary system and biliary injury have been summarized. Additionally, it provides an overview of novel biomarkers and therapeutic strategies that are currently being investigated. Although expectations of NMP to adequately assess biliary injury are high, scant literature is available. There are several biomarkers that can be measured in bile that have been associated with outcomes after transplantation, mainly including pH and electrolytes. However, proper validation of those and other novel markers and investigation of the pathophysiological effect of NMP on the biliary tree is still warranted.
Dead or Alive? A Review of Perinatal Factors That Determine Canine Neonatal Viability
Authors: Uchańska Oliwia , Ochota Małgorzata , Eberhardt Maria , Niżański Wojciech
Published: May 2022

The perinatal period has a critical impact on viability of the newborns. The variety of factors that can potentially affect the health of a litter during pregnancy, birth, and the first weeks of life requires proper attention from both the breeder and the veterinarian. The health status of puppies can be influenced by various maternal factors, including breed characteristics, anatomy, quality of nutrition, delivery assistance, neonatal care, and environmental or infectious agents encountered during pregnancy. Regular examinations and pregnancy monitoring are key tools for early detection of signals that can indicate disorders even before clinical signs occur. Early detection significantly increases the chances of puppies’ survival and proper development. The purpose of the review was to summarize and discuss the complex interactions between all elements that, throughout pregnancy and the first days of life, have a tangible impact on the subsequent fate of the offspring. Many of these components continue to pose challenges in veterinary neonatology; thus, publications presenting the current state of knowledge in this field are in demand.
Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter Genes
Authors: Gebeyew Kefyalew , Jiang Chunyu , Gao Qinghua , Zhang Liping , Zhu Hanhua , Tian Yushi , Wang Qi , Wei Yuqing , Tan Zhiliang , Han Xuefeng
Published: May 2022

Metal transporters, including divalent metal-ion transporter-1 (DMT1), Zrt-/Irt-like protein 8 and 14 (ZIP8 and ZIP14), and ferroportin-1 (FPN1), reportedly participate in cellular cadmium (Cd) uptake, but those in farm animals remain unclarified. This study aimed to examine the growth, plasma biochemical indices, Cd accumulation, and expression of metal transporter genes in the liver, kidney, and muscle of goats exposed to rice paddies contaminated with different levels of Cd. Twenty-four goats were randomly assigned across three dietary treatments: 0.23, 0.63, and 1.07 mg of Cd/kg of dry matter (DM) for 60 days. The results showed that dietary Cd exposure increased (p < 0.05) both Cd accumulation and the mRNA expressions of metal transporter genes (DMT1, ZIP, and FPN1) in the liver and kidney but not in the muscle, suggesting dietary Cd exhibited different deposition rates between goat liver, kidney, and muscle. These outcomes suggest that high levels of dietary Cd stimulated the expression of metal transporter genes and thereby enhanced the uptake and accumulation of Cd in the goat liver and kidney. As such, higher Cd concentrations in the liver and kidney observed with Cd diets could be partly explained by upregulation of metal transport genes expression.
Impact of Indigestible Materials on the Efficiency of Fecal Corticosterone Immunoassay Testing in Pituophis Species
Authors: Racine Holly , Guthrie Kinsey Skalican , Hill Tyler , Loughman Zachary
Published: May 2022

Measuring fecal glucocorticoid metabolites (FGM) has recently become a sought-after method for assessing stress in animals. While there are many benefits to this methodology, there are also recognized limitations, including the apprehensive interpretation of results. While many factors can influence FGM levels, we aimed to standardize and improve these methods in snakes. Fecal samples were collected from Pituophis species and FGMs were extracted by two different sample collection methods: (1) fecal sample containing undigested materials and (2) fecal samples with undigested materials removed. These extracts were then used to quantify FGM concentrations using a corticosterone EIA kit. The results indicated that the samples with the undigestible materials removed had a 95% increase in overall yield (p < 0.01). Since the collected fecal samples contain 75% undigestible materials by weight, these results support our hypothesis that removing these materials will improve extraction methods for a more reliable measurement of corticosterone. This is the first step towards standardizing the methods for assessing stress by measuring fecal glucocorticoid metabolites in snakes.
Cats and SARS-CoV-2: A Scoping Review
Authors: Doliff Ramona , Martens Pim
Published: May 2022

Since the beginning of the COVID-19 pandemic, various animal species were found to be susceptible to SARS-CoV-2 infection. The close contact that exists between humans and cats warrants special attention to the role of this species. Therefore, a scoping review was performed to obtain a comprehensive overview of the existing literature, and to map key concepts, types of research, and possible gaps in the research. A systematic search of the databases PubMed, Google Scholar, and Scopus and the preprint servers medRxiv and bioRxiv was performed. After a two-step screening process, 27 peer-reviewed articles, 8 scientific communication items, and 2 unpublished pre-prints were included. The main themes discussed were susceptibility to SARS-CoV-2, induced immunity, prevalence of infection, manifestation of infection, interspecies transmission between humans and cats, and lastly, intraspecies transmission between cats. The main gaps in the research identified were a lack of large-scale studies, underrepresentation of stray, feral, and shelter cat populations, lack of investigation into cat-to-cat transmissions under non-experimental conditions, and the relation of cats to other animal species regarding SARS-CoV-2. Overall, cats seemingly play a limited role in the spread of SARS-CoV-2. While cats are susceptible to the virus and reverse zoonotic transmission from humans to cats happens regularly, there is currently no evidence of SARS-CoV-2 circulation among cats.
Metabonomic Analysis Provides New Insights into the Response of Zhikong Scallop (Chlamys farreri) to Heat Stress by Improving Energy Metabolism and Antioxidant Capacity
Authors: Dong Xixi , Yang Zujing , Liu Zhi , Wang Xuefeng , Yu Haitao , Peng Cheng , Hou Xiujiang , Lu Wei , Xing Qiang , Hu Jingjie , Huang Xiaoting , Bao Zhenmin
Published: May 2022

Temperature is an important factor affecting the growth, development and survival of marine organisms. A short episode of high temperature has been proven to be a severe threat to sustainable shellfish culture. Zhikong scallop (Chlamys farreri), a shellfish with broad economic and biological value in North China, has frequently experienced heat stress in summer in recent years. To understand the effects of heat stress on shellfish, the metabolism of C. farreri was analyzed after exposure to 27 °C for either 6 h or 30 d. After 6 h of heat stress exposure, a total of 326 and 264 significantly different metabolites (SDMs) were identified in gill and mantle tissues, respectively. After 30 d of heat stress exposure, a total of 381 and 341 SDMs were found in the gill and mantle tissues, respectively. These SDMs were mainly related to the metabolism of amino acids, carbohydrates, lipids and nucleotides. A decline in pyruvic acid, and an increase in citric acid and fumaric acid in the gills and mantle of C. farreri indicated an alteration in energy metabolism, which may be attributed to increased ATP production in order to overcome the heat stress. Among the SDMs, 33 metabolites, including pyruvic acid, glycine and citric acid, were selected as potential biomarkers for heat stress response in C. farreri. In addition, a decline in glutamine and β-Alanine levels indicated oxidative stress in C. farreri exposed to heat, as well as an increase in the total antioxidant capacity (T-AOC). Our findings suggested C. farreri have the potential to adapt to heat stress by regulating energy metabolism and antioxidant capacity.
Human-Robot Interaction Torque Estimation Methods for a Lower Limb Rehabilitation Robotic System with Uncertainties
Authors: Yepes Juan C. , Rúa Santiago , Osorio Marisol , Pérez Vera Z. , Moreno Jaime A. , Al-Jumaily Adel , Betancur Manuel J.
Published: May 2022

Lower limb rehabilitation robot (LLRR) users, to successfully conduct isotonic exercises, require real-time feedback on the torque they exert on the robot to meet the goal of the treatment. Still, direct torque measuring is expensive, and indirect encoder-based estimation strategies, such as inverse dynamics (ID) and Nonlinear Disturbance Observers (NDO), are sensitive to Body Segment Inertial Parameters (BSIPs) uncertainties. We envision a way to minimize such parametric uncertainties. This paper proposes two human–robot interaction torque estimation methods: the Identified ID-based method (IID) and the Identified NDO-based method (INDO). Evaluating in simulation the proposal to apply, in each rehabilitation session, a sequential two-phase method: (1) An initial calibration phase will use an online parameter estimation to reduce sensitivity to BSIPs uncertainties. (2) The torque estimation phase uses the estimated parameters to obtain a better result. We conducted simulations under signal-to-noise ratio (SNR) = 40 dB and 20% BSIPs uncertainties. In addition, we compared the effectiveness with two of the best methods reported in the literature via simulation. Both proposed methods obtained the best Coefficient of Correlation, Mean Absolute Error, and Root Mean Squared Error compared to the benchmarks. Moreover, the IID and INDO fulfilled more than 72.2% and 88.9% of the requirements, respectively. In contrast, both methods reported in the literature only accomplish 27.8% and 33.3% of the requirements when using simulations under noise and BSIPs uncertainties. Therefore, this paper extends two methods reported in the literature and copes with BSIPs uncertainties without using additional sensors.