bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the association between household exposure to Anopheles stephensi and malaria in Sudan and Ethiopia: A case-control study protocol
Authors: Ashine Temesgen , Ebstie Yehenew Asmamaw , Ibrahim Rayyan , Epstein Adrienne , Bradley John , Nouredayem Mujahid , Michael Mikiyas G. , Sidiahmed Amani , Negash Nigatu , Kochora Abena , Sulieman Jihad Eltaher , Reynolds Alison M. , Alemayehu Eba , Zemene Endalew , Eyasu Adane , Dagne Alemayehu , Hailemeskel Elifaged , Jaiteh Fatou , Geleta Dereje , Lejore Ephrem , Weetman David , Hussien Ahmed Mahmoud , Saad Fadwa , Assefa Gudissa , Solomon Hiwot , Bashir Abdelgadir , Massebo Fekadu , Peeters Koen , Yewhalaw Delenasaw , Kafy Hmooda Toto , Donnelly Martin J. , Gadisa Endalamaw , Malik Elfatih M. , Wilson Anne L.
Published: September 2024

Background

Endemic African malaria vectors are poorly adapted to typical urban ecologies. However, Anopheles stephensi, an urban malaria vector formerly confined to South Asia and the Persian Gulf, was recently detected in Africa and may change the epidemiology of malaria across the continent. Little is known about the public health implications of An. stephensi in Africa. This study is designed to assess the relative importance of household exposure to An. stephensi and endemic malaria vectors for malaria risk in urban Sudan and Ethiopia.

Methods

Case-control studies will be conducted in 3 urban settings (2 in Sudan, 1 in Ethiopia) to assess the association between presence of An. stephensi in and around households and malaria. Cases, defined as individuals positive for Plasmodium falciparum and/or P. vivax by microscopy/rapid diagnostic test (RDT), and controls, defined as age-matched individuals negative for P. falciparum and/or P. vivax by microscopy/RDT, will be recruited from public health facilities. Both household surveys and entomological surveillance for adult and immature mosquitoes will be conducted at participant homes within 48 hours of enrolment. Adult and immature mosquitoes will be identified by polymerase chain reaction (PCR). Conditional logistic regression will be used to estimate the association between presence of An. stephensi and malaria status, adjusted for co-occurrence of other malaria vectors and participant gender.

Conclusions

Findings from this study will provide evidence of the relative importance of An. stephensi for malaria burden in urban African settings, shedding light on the need for future intervention planning and policy development.
Identify the digitalization technology opportunity of low-carbon energy technologies: Using the patent data and collaborative filtering
Authors: Liu Jie , Cai Wanlin
Published: September 2024

The digitalization of low-carbon energy technologies (LCET) provides important technical support for the transition to a greener energy system. Digitalization addresses the phenomenon of the growing application of information and communications technologies (ICT) across the economy, which is regarded as the technology convergence between ICT and other technologies. Scholars have revealed the signs that LCET and ICT are becoming increasingly interlinked, which raises the challenges for predicting and identifying the technology opportunities for innovations in the converged technology area. To address the challenges, this paper proposes a collaborative filtering approach to identify the digitalization technology opportunity of low-carbon energy technologies using patent classification and patent citation information. We applied the proposed collaborative filtering approach using a large LCET patent dataset derived from the United States Patent and Trademark Office (USPTO). The results indicate that the proposed method can effectively identify digitalization technology opportunities of LCET, and the current LCET digitalization technology opportunities identified based on this approach are mainly concentrated in the Energy storage field. The advantages of the proposed approach are that its underlying data are more readily available and its technical complexity is relatively lower, and thus, more replicable for other technology fields.
Urinary metabolic profile and its predictive indexes after MSG consumption in rat
Authors: Sukmak Manatsaphon , Kyaw Thin Su , Nahok Kanokwan , Sharma Amod , Silsirivanit Atit , Lert-itthiporn Worachart , Japrung Deanpen , Pinlaor Somchai , Anutrakulchai Sirirat , Selmi Carlo , Slupsky Carolyn M. , Hammock Bruce D. , Cha’on Ubon
Published: September 2024

Monosodium glutamate (MSG) is a widely used food additive with conflicting evidence regarding its potential effects on human health, with proposed relevance for obesity and metabolic syndrome (MetS) or chronic kidney disease. As being able to accurately quantify the MSG dietary intake would help clarify the open issues, we constructed a predictive formula to estimate the daily intake of MSG in a rat model based on the urinary metabolic profile. Adult male Wistar rats were divided into groups receiving different daily amounts of MSG in drinking water (0.5, 1.5, and 3.0 g%), no MSG, and MSG withdrawal after 3.0% MSG treatment for 4 weeks. We then analyzed 24-hour urine samples for chemistries and metabolites using 1H NMR spectrometry and observed a strong correlation between urine pH, sodium, bicarbonate, alpha-ketoglutarate, citrate, fumarate, glutamate, methylamine, N-methyl-4-pyridone-3-carboxamide, succinate, and taurine and the daily MSG intake. Following the multiple linear regression analysis a simple formula model based on urinary Na+, citrate, and glutamate was most accurate and could be validated for estimating daily MSG intake. In conclusion, we propose that the daily MSG intake correlates with urinary metabolites in a rat model and that this new tool for monitoring the impact of MSG on health measures.
The role of mental health in the relationship between nursing care satisfaction with nurse-patient relational care in Chinese emergency department nursing
Authors: Huang Hui , Cui Jing , Zhang Hua , Gu Yuhui , Ni Haosheng , Meng Ya
Published: September 2024

Background

The relationship between a nurse and a patient is a key part of nursing that can impact how happy the patient is with the care they receive. It appears that the nurse’s mental health can also affect this connection. However, there is little research on this topic. So, the aim of the present study was to determine the correlation of nurse’s mental health with nurse–patient relational care and nursing care satisfaction.

Methods

A total of 532 nurses and 532 patients from 13 Level-III hospitals of Hubei province (China) completed a China Mental Health Survey, general information questionnaire, the Nursing Care Satisfaction Scale, and Relational Care Scale.

Results

Age, nurse working years, and night shift last month were correlated with mental health score (r = -0.142, r = -0.150, r = 0.164, p < 0.05). Nurse’s mental health was correlated with relational care score and nursing care satisfaction score (r = -0.177, r = -0.325, p < 0.05). Also, relational care score, patients age and gender were correlated with nursing care satisfaction score (r = 0.584 and r = 0.143, x2 = 11.636, p < 0.05). Descriptive information of nurses had a direct impact on nurses’ mental health (direct effect = 0.612, 0.419–0.713). Nurses’ mental health had a direct effect on relational care score (direct effect = 0.493, 0.298–0.428) and an indirect effect on nursing care satisfaction score (indirect effect = 0.051, 0.032–0.074). Relational care score and patient’s descriptive information had also a direct effect on nursing care satisfaction score (direct effect = 0.232, 0.057–0.172 and 0.057, 0.347–0.493).

Conclusion

This study showed that the better the mental health of nurses, the more patients feel satisfied with nursing services.
A nonhuman primate model for genital herpes simplex virus 2 infection that results in vaginal vesicular lesions, virus shedding, and seroconversion
Authors: Wang Kening , Jordan Tristan , Dowdell Kennichi , Herbert Richard , Moore Ian N. , Koelle David M. , Cohen Jeffrey I.
Published: September 2024

The most commonly used animal models for evaluating the efficacy of HSV-2 candidate vaccines are mice and guinea pigs. While numerous HSV-2 vaccine candidates have been tested in these animals and were effective in reducing disease and mortality, these results did not predict the effectiveness of the vaccines in human trials. Infection of rhesus macaques rarely results in lesions or HSV-2 specific antibody responses. In seeking an animal model that better recapitulates human disease and that might be more predictive of the efficacy of prophylactic vaccines than mice and guinea pigs, we evaluated Cebus apella (C. apella), a New World primate, in an HSV-2 genital infection model. Infectious HSV-2 was cultured from vaginal swabs from all 4 animals for 9–14 days after intravaginal inoculation of HSV-2 seronegative monkeys. Two of 4 monkeys had vesicular lesions in the vagina or vulva. No neurological symptoms were noted. Recurrent lesions and HSV-2 DNA shedding after acute disease resolved was infrequent. UV irradiation of the genital area did not induce recurrent genital lesions or virus shedding. All 4 monkeys developed HSV-2 neutralizing antibodies as well as virus-specific CD4 and CD8 T cell responses. Reinfection of animals 15 to 19 months after primary infection did not result in lesions; animals had reduced virus shedding and a shorter duration of shedding compared with that during primary infection, suggesting that primary infection induced protective immunity. Primary fibroblasts from C. apella monkeys supported the growth of HSV-2 in vitro; in contrast, HSV-2 did not replicate above the titer of the input inoculum in fibroblasts from rhesus macaques. These observations suggest that the C. apella monkey has potential to serve as a model for evaluating the efficacy of prophylactic vaccines, antivirals, or monoclonal antibodies to HSV-2.
Norovirus-mediated translation repression promotes macrophage cell death
Authors: Aktepe Turgut E. , Deerain Joshua M. , Hyde Jennifer L. , Fritzlar Svenja , Mead Eleanor M. , Carrera Montoya Julio , Hachani Abderrahman , Pearson Jaclyn S. , White Peter A. , Mackenzie Jason M.
Published: September 2024

Norovirus infection is characterised by a rapid onset of disease and the development of debilitating symptoms including projectile vomiting and diffuse diarrhoea. Vaccines and antivirals are sorely lacking and developments in these areas are hampered by the lack of an adequate cell culture system to investigate human norovirus replication and pathogenesis. Herein, we describe how the model norovirus, Mouse norovirus (MNV), produces a viral protein, NS3, with the functional capacity to attenuate host protein translation which invokes the activation of cell death via apoptosis. We show that this function of NS3 is conserved between human and mouse viruses and map the protein domain attributable to this function. Our study highlights a critical viral protein that mediates crucial activities during replication, potentially identifying NS3 as a worthy target for antiviral drug development.
Simulation of Groundwater Potential Zone Using Geographic Information System (GIS) Through Related Factors
Authors: Olusami Joel Olusoji , Familusi Ayokunle Oluwaseun , Olatunji Abass Adekunle , Ogundare Damilola Ayodele , Akindele Akinsanya Ebenezer , Akinyele Isikilu Oyewole
Published: September 2024

Groundwater is the primary water source for irrigating and drinking in dry seasons. This work was examined to assess groundwater potential area underlain by basement complex with GIS based approach of selected locations in Osogbo. The soil samples were taken and soil textural and permeability analyses were done in the laboratory. Supervised classification analysis was done on LandSAT imagery to generate Land use and a lineament map, surface analyst algorithm was applied on ASTER dam to generate elevation map. Hydraulic conductivity and overburden thickness maps were generated from the Borehole data parameters. Interpolation method was used to generate spatially spread clay, silt, sand, rainfall and hydraulic conductivity. Multi-criteria analysis was used to generate groundwater potential zone map in the study area. Specific factors were considered for groundwater potential zone. Reclassification was done on each of the considered factors and AHP model was used to assign weight to all the factors. The results of the various factors responsible for groundwater potential include  lineament density 0-0.0028; sand 67.2-89.60%, silt 9.00-30.00%, clay 0.17-2.80%, permeability 0.000010-0.00002, rainfall 1304.02-1469.06mm/yr, hydraulic conductivity 0.21-0.96m/min, land use consisting of degraded forest 48.65%, riparian forest 13.05%, built up 38.29%, and water body 0.001%. However, ground water potential of the study area was assessed and classified into fair, good and very good with the coverage of 2%, 80% and 18% respectively. Conclusively, the groundwater recharge potential is very good at Ofatedo, Gbonmi and Oke-Oro locations, good at Awosuru, Gbodofon and Dagbolu locations, but fair at Mallam Tope location in Osogbo
EEG Based Emotion Analysis using Reinforced Spatio-Temporal Attentive Graph Neural and Context Net Techniques
Authors: Akalya Devi C. , Karthika Renuka D.
Published: September 2024

EEG-based emotion Classification is used to distinguish and observe the mental state or emotions. Emotion classification using EEG is used for medical, security, and various other purposes. There are several deep learning and machine learning techniques that are used to classify EEG emotion signals. They do not provide sufficient accuracy and have higher complexity and a high error rate. In this manuscript, a novel Reinforced Spatio-Temporal Attentive Graph Neural Networks (RSTAGNN)and ContextNet for emotion classification with EEG signals is proposed (RSTAGNN-ContextNet-GWOA-EEG-EA). Here the input EEG signals are taken from two benchmark datasets namely as DEAP and K-EmoCon datasets. Then the input EEG signals are pre-processed and the features are extracted using ContextNet with Global principal component Analysis (GPCA). After that, the EEG signal emotions are classified using Reinforced Spatio-Temporal Attentive Graph Neural Networks (RSTAGNN) method. Then the weight parameters of the RSTAGNN are optimized using Glowworm Swarm Optimization Algorithm (GWOA). The proposed model classifies the EEG signal emotions with high accuracy. The performance of the proposed method using DEAP dataset attains higher accuracy by 24.05%, 12.64%relatedto existing systems, like DWT-SVM-EEG-EA-DEAP and GCNN-LSTM-EEG-EA-DEAP respectively and the performance of the proposed system using K-EmoCon dataset attains higher accuracy 32.64%, 15.65% related to existing systems, like SigRep-EEG-EA-K-
EEG Based Emotion Analysis using Reinforced Spatio-Temporal Attentive Graph Neural and Context Net Techniques on Deap and K- EmoCon Article and CAT-EEG-EA-K-EmoCon respectively.
Autoimmune thyroiditis — what is new?
Authors: Kazakova M. P. , Tskaeva A. A. , Starostina E. A. , Troshina E. A.
Published: September 2024

Recently, the prevalence of autoimmune diseases has been steadily increasing among the population, posing a significant problem for healthcare. One of the most common autoimmune pathologies is autoimmune thyroiditis (AIT). Due to the absence of prognostic markers to predict outcomes of AIT, such as the development of hypothyroidism, there is an increasing necessity for molecular-genetic research into the autoimmune changes occurring within this disease. This research is likely to contribute to the development of new diagnostic methods and the identification of key aspects of pathogenesis, thereby preventing the development of the autoimmune process. One area of research includes analyzing the impact of environmental factors on the autoimmune process. Investigating the relationships between factors and stages of AIT pathogenesis can form the basis for methods aimed at modifying these factors to prevent disease progression. Studying the comorbidity of autoimmune diseases and the relationships between various thyroid pathologies of different etiologies, helps identify high-risk groups for subsequent screening and early detection. Thus, exploring the molecular-biological basis of AIT development lays the foundation for developing effective strategies in prevention, diagnosis, and therapy, aimed at improving the quality of life of patients.
Sujeto, psicoanálisis y política
Authors: Yagüe Pedro Guillermo
Published: September 2024

En el presente artículo nos proponemos analizar el modo en que el filósofo argentino León Rozitchner (1924-2011) lee de manera crítica los trabajos teóricos de Lacan. En este sentido, describiremos la lectura que realiza de los textos del psicoanalista francés, las razones por las que sostiene que su regreso a Freud excluye algunos de los elementos más revolucionarios del psicoanálisis. En la primera parte del trabajo, nos centraremos en la forma en que Rozitchner establece una diferencia con Lacan en lo que respecta a los inicios de la constitución histórica del sujeto. Allí se contrapondrán dos ideas bien diferentes sobre el comienzo de la vida humana: el lleno materno contra la dehiscencia constitutiva. Esto nos permitirá avanzar, en la segunda parte del artículo, sobre la crítica que el filósofo argentino realiza de la caracterización del Edipo en tres tiempos, propia del pensamiento lacaniano. Rozitchner sostendrá que Lacan excluye elementos cruciales de las investigaciones que Freud realiza sobre el complejo parental. Una vez analizados estos dos puntos, nos detendremos en las consecuencias políticas que Rozitchner deduce de estas diferencias con Lacan.
Nachhaltige Männlichkeit als Grundvoraussetzung für eine ­gelingende sozial-ökologische Transformation
Authors: Schölper Dag , Schwerma Klaus
Published: September 2024

Transformation bedeutet Veränderung, und das heißt ­auf ­vielen ­Ebenen, dass Menschen liebgewonnene und eingeschliffene ­Verhaltensweisen infrage stellen müssen. Männlichkeit ist nicht ­einfach ein Bündel neutral ­beschreibender Attribute dessen, was männlich ist. ­Männlichkeit ist eine diskursive Kampfarena, und ­heute vorherrschende Bilder von Männlichkeit stehen ­teils in einem radikalen Widerspruch zu Nachhaltigkeit.
Trojan Nobodies: Uncarnal Knowledge in Troilus and Criseyde
Authors: Arner Timothy D.
Published: September 2024

This article considers the distinct lack of bodies and embodiment throughout Chaucer’s Troilus and Criseyde. While it seems to be a war-time erotic romance in which the plot hinges on the physical threats and pleasures available to its characters, through its many contradictions and deceptions, the poem makes the bodies of Troilus, Criseyde, and Pandarus unknowable, even at their most crucial moments. It is through the frustration of bodily hermeneutics in Troilus and Criseyde that Chaucer signals his rejection of the romance genre and the beginning of an anti-Boethian turn that becomes the governing theme of the first fragment of the Canterbury Tales.
Getting in Touch with Troy in Fifteenth-Century France: The First Prose Version of the Roman de Troie in the Manuscript F°26 of the Abbey of Maredsous
Authors: Rochebouet Anne
Published: September 2024

This paper considers how the codex as a medium, specifically via its illustration and layout, translates into material forms the literary and textual choices of the texts it transmits in order to make its version of the Troy story tangible, and thus intelligible for its medieval readers. It takes as an example the first prose version of the Roman de Troie of Benoît de Sainte-Maure, probably composed in the last quarter of the thirteenthcentury in Frankish Greece, and focuses more specifically on a late manuscript of Prose 1, recently rediscovered, the manuscript F°26 from the Abbey of Maredsous (Belgium), made almost two centuries later, c. 1450, in the Burgundian Netherlands. This paper argues that this codex, in its specific context of production and within its textual and visual traditions, allow its audience to come into contact with the long-lost city of Troy, and the narrative that evokes it.
The role of Cardiotrophin-1 and echocardiography in early detection of subclinical diabetic cardiomyopathy in children and adolescents with type 1 diabetes mellitus
Authors: Hassanein Samah A. , Hassan Mona M. , Samir Mohamed , Aboudeif Mahmoud O. , Thabet Mohamed S. , Abdullatif Mona , Khedr Dina
Published: September 2024

Objectives

To assess the role of Cardiotrophin-1 (CT-1) and echocardiography in early detection of subclinical Diabetic Cardiomyopathy (DCM) in children with type 1 Diabetes Mellitus (T1D).

Methods

This case-control study included two groups of children and adolescents aged between 7 and 18. Group (1) included forty patients with T1D (duration > 5 years) regularly followed at the children's hospital of Cairo University, and Group (2) included forty age and sex-matched healthy subjects as a control group. The serum level of CT-1 was measured, and conventional echocardiography, tissue Doppler imaging (TDI), and 2D speckle tracking echocardiography were performed.

Results

The level of CT-1 in the cases ranged from 11 to 1039.4 pg/ml with a median (IQR) of 19.4 (16.60–25.7) pg/ml, while its level in the control group ranged from 10.8 to 162.6 pg/ml with a median (IQR) of 20.2 (16.2–24.8) pg/ml. CT-1 levels showed no statistically significant difference between cases and controls. Patients had significantly higher mean left ventricle E/E′ ratio (p<0.001), lower mean 2D global longitudinal strain (GLS) of the left ventricle (LV) (p<0.001), and lower mean GLS of the right ventricle (RV) (p<0.001) compared to controls. Ofpatients with diabetes, 75 % had LV diastolic dysfunction, 85 % had RV diastolic dysfunction, 97.5 % had LV systolic dysfunction, and 100 % had RV systolic dysfunction.

Conclusions

Non-conventional echocardiography is important for early perception of subclinical DCM in patients with T1D. Cardiotrophin-1 was not specific for early detection of DCM.
ACSL4 mediates inflammatory bowel disease and contributes to LPS-induced intestinal epithelial cell dysfunction by activating ferroptosis and inflammation
Authors: Lam Ieng-Hou , Chan Chon-In , Han Meixia , Li Lixuan , Yu Hon-Ho
Published: September 2024

Background

The pathogenesis of inflammatory bowel disease (IBD) is closely associated with the dysfunction of the intestinal epithelial barrier, leading to increased bacterial translocation, leukocyte infiltration, and mucosal injury, which may act as a pivotal or incipient event in the pathophysiology of the disorder. The primary objective of this study is to examine the key genes implicated in IBD and the perturbation of intestinal epithelial cell function.

Methods

The genes associated with ferroptosis were identified through the utilization of the Gene Expression Omnibus (GEO) database and the GeneCard database. Additionally, an in vitro model of IBD was established by stimulating Caco-2 cells with lipopolysaccharides (LPSs) to investigate the molecular mechanisms underlying intestinal epithelial cell dysfunction.

Results

We discovered evidence that establishes a connection between ferroptosis and the inflammatory responses associated with the development of IBD. This evidence suggests that IBD patients who exhibit an inflammatory response have higher expression of the acyl-CoA synthetase long-chain family member 4 (ACSL4) gene compared to IBD patients without an inflammatory response or healthy individuals. Exposure to LPS at concentrations of 1 or 10 μg/mL resulted in a significant upregulation of ferroptosis-related genes ACSL4, GPX4, and SLC7A11, as well as an increase in ferroptosis biomarkers MDA and a decrease in CAT and GSH-Px levels compared to the control group. Inhibition of ACSL4 using si-ACSL4 or rosiglitazone demonstrated protective effects against LPS-induced ferroptosis and NF-κB-mediated inflammatory response.

Conclusion

ACSL4 shows potential as a promising target for ferroptosis in the prevention and treatment of IBD and dysfunction of intestinal epithelial cells.
Optimierung von Subsumtionsprozessen
Authors: Krebs Moritz
Published: September 2024

Sowohl Formen der Optimierung als auch der Entgrenzung lassen sich mit Hilfe Marx’scher Begriffe fassen. In diesem Artikel wird skizziert, welche Bedeutung einer gleichermaßen gesellschaftstheoretischen und anthropologischen Verortung dieser kaum zu übersehenden Tendenzen im Zusammenhang mit den veränderten Weltbeziehungen im Kapitalozän zukommt.
Beyond Glycolysis: Aldolase A Is a Novel Effector in Reelin-Mediated Dendritic Development
Authors: Lagani Gavin D. , Sha Mingqi , Lin Weiwei , Natarajan Sahana , Kankkunen Marcus , Kistler Sabrina A. , Lampl Noah , Waxman Hannah , Harper Evelyn R. , Emili Andrew , Beffert Uwe , Ho Angela
Published: September 2024

Reelin, a secreted glycoprotein, plays a crucial role in guiding neocortical neuronal migration, dendritic outgrowth and arborization, and synaptic plasticity in the adult brain. Reelin primarily operates through the canonical lipoprotein receptors apolipoprotein E receptor 2 (Apoer2) and very low-density lipoprotein receptor (Vldlr). Reelin also engages with noncanonical receptors and unidentified coreceptors; however, the effects of which are less understood. Using high-throughput tandem mass tag (TMT) liquid chromatography tandem mass spectrometry (LC-MS/MS)-based proteomics and gene set enrichment analysis (GSEA), we identified both shared and unique intracellular pathways activated by Reelin through its canonical and noncanonical signaling in primary murine neurons of either sex during dendritic growth and arborization. We observed pathway cross talk related to regulation of cytoskeleton, neuron projection development, protein transport, and actin filament-based process. We also found enriched gene sets exclusively by the noncanonical Reelin pathway including protein translation, mRNA metabolic process, and ribonucleoprotein complex biogenesis suggesting Reelin fine-tunes neuronal structure through distinct signaling pathways. A key discovery is the identification of aldolase A, a glycolytic enzyme and actin-binding protein, as a novel effector of Reelin signaling. Reelin induced de novo translation and mobilization of aldolase A from the actin cytoskeleton. We demonstrated that aldolase A is necessary for Reelin-mediated dendrite growth and arborization in primary murine neurons and mouse brain cortical neurons. Interestingly, the function of aldolase A in dendrite development is independent of its known role in glycolysis. Altogether, our findings provide new insights into the Reelin-dependent signaling pathways and effector proteins that are crucial for dendritic development.
Membrane progesterone receptors mediate progesterone-stimulated glycogenolysis in the bovine uterine epithelium
Authors: Berg Malia D , Dean Matthew
Published: September 2024

In livestock, the amount of glucose needed by the endometrium and embryo increases during early pregnancy. Yet, how glucose concentrations in the endometrium are regulated remains unclear. The bovine uterine epithelium can store glucose as glycogen, and glycogen content decreases in the luteal phase. Our objective was to elucidate the role of progesterone in glycogen breakdown in immortalized bovine uterine epithelial (BUTE) cells. After 48 h of treatment, progesterone decreased glycogen abundance in BUTE cells (P < 0.001) but did not alter glycogen phosphorylase levels. RU486, a nuclear progesterone receptor (nPR; part of the PAQR family) antagonist, did not block progesterone’s effect, suggesting that progesterone acted through membrane progesterone receptors (mPRs). RT-PCR confirmed that BUTE cells express all five mPRs, and immunohistochemistry showed that the bovine uterine epithelium expresses mPRs in vivo. An mPRα agonist (Org OD 02-0) reduced glycogen abundance in BUTE cells (P < 0.001). Progesterone nor Org OD 02-0 affected cAMP concentrations. Progesterone increased phosphorylated AMP-activated protein kinase (pAMPK) levels (P < 0.001), indicating that progesterone increases intracellular AMP concentrations. However, AMPK did not mediate the effect of progesterone. AMP allosterically activates glycogen phosphorylase, and D942 (which increases intracellular AMP concentrations) decreased glycogen abundance in BUTE cells. A glycogen phosphorylase inhibitor partially blocked the effect of progesterone (P < 0.05). Progesterone and Org OD 02-0 had similar effects in Ishikawa cells (P < 0.01), a human cell line that lacks nPRs. In conclusion, progesterone stimulates glycogen breakdown in the uterine epithelium via mPR/AMP signaling. Glucose released from glycogen could support embryonic development or be metabolized by the uterine epithelium.
SAĞLIK HİZMETLERİNİN FİNANSMANINDA DEVLETİN MALİ YÜKÜMLÜLÜĞÜNÜN ANAYASAL ÇERÇEVESİ
Authors: Yazıcıoğlu İsmail
Published: September 2024

Sağlık hakkı, anayasalarda ve pek çok uluslararası hukuk metinlerinde yer verilen sosyal bir haktır. Sosyal hakların güvence altına alındığı, sosyal devlet anlayışını benimseyen anayasalarda sağlık hakkı bakımından devletlerin olumlu edimde bulunması gerekmektedir. 1982 Anayasası da sosyal haklar arasında, 56. maddede sağlık hakkıyla ilgili düzenlemelere yer vererek sağlık hizmetlerinin yerine getirilmesi bakımından devletin yükümlülüklerinin bulunduğunu kabul etmiştir. Bu yükümlülüklerin bir maliyet ortaya çıkaracağı gerçeği karşısında, Anayasanın 65. maddesinde devletin sosyal haklardan doğan yükümlülüklerini yerine getirmesinin sınırı düzenlenmiş ve devlete bu alanlardaki görevlerini öncelikler gözeterek mali kaynaklarının yeterliliği ölçüsünde yerine getirme imkânı verilmiştir. Bu çalışmada Anayasadaki düzenlemeler ve Anayasa Mahkemesi kararları doğrultusunda inceleme yapılarak, yaşama, maddi ve manevi varlığı koruma hakkı gibi klasik haklarla yakın ilişkili olan sağlık hakkının öncelikli olarak korunması, sağlık hizmetlerinin yerine getirilmesinde devletin mali yükümlülüklerinin kapsamının diğer sosyal haklardan daha geniş güvencelere kavuşturulması gerektiği ileri sürülmektedir.
Multinomial logit death forecasting model
Authors: Jakaitienė Audronė
Published: September 2024

The multinomial regression logit model is analyzed. The algorithms and software are made for this model in order to get estimation of parameters. Calculations are made using generated population of 1000 cases.
High level of RNA HAS2-AS1 in the buffy coat of a patient blood sample is a more informative prognostic marker of COVID-19 clinical course compared to the level of hyaluronic acid in plasma
Authors:   Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Dubrovskyi E. , Drevytska T. ,   Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;, Dosenko V. ,   Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;, Stroy D. ,   Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
Published: September 2024

Numerous studies have confirmed the association of COVID-19 clinical course with the blood levels of hyaluronic acid and long non-coding RNA HAS2-AS1 as a positive regulator of hyaluronan synthase. We aimed to estimate both the level of hyaluronic acid in plasma and the level of RNA HAS2-AS1 in leukocytes (buffy coat) from the same blood sample at the time of patient admission to the hospital and to analyze the specificity of these indicators as biomarkers of COVID-19 severity. The study involved 78 patients with confirmed COVID-19 who underwent treatment between 2020 and 2021 at the Kyiv City Clinical Hospital N 4. The patients were divided into three groups based on the severity of the disease and classified as mild (n = 12), moderate (n = 36), and severe (n = 30). The level of hyaluronic acid in plasma was determined using the Hyaluronic Acid ELISA kit “Abcam” (USA). The buffy coat was isolated by centrifugation of the blood stabilized with EDTA-K and further aspiration of the leukocyte “cloud”. The expression level of HAS2-AS1 in buffy coat leukocytes was estimated using reverse transcription and real-time PCR. According to the obtained data, the level of hyaluronic acid in the plasma of patients with moderate and severe illness was 1.5 and 2.2 times higher compared to the mild illness group, respectively. Meanwhile, the level of RNA HAS2-AS1 in blood lymphocytes (buffy coat) of patient with moderate and severe illness was increased by 7.7 and 22.6 times compared to patients with mild illness. The results of our study demonstrate that, unlike the level of hyaluronic acid in plasma, the level of HAS2-AS1 in a buffy coat is a more reliable prognostic criterion for severe COVID-19 and allows distinguishing patients with varying clinical severity during hospitalization. Keywords: buffy coat, COVID-19, hyaluronic acid, leukocytes, plasma, RNA HAS2-AS1
New combinations in Anthoxanthum (Poaceae) for Aotearoa / New Zealand taxa earlier placed in Hierochloe
Authors:   School of Environmental & Animal Sciences, Unitec Institute of Technology, Private Bag 92025, Victoria Street West, Auckland 1142, Aotearoa / New Zealand, de Lange P.J. , James C.J. ,   School of Environmental & Animal Sciences, Unitec Institute of Technology, Private Bag 92025, Victoria Street West, Auckland 1142, Aotearoa / New Zealand
Published: September 2024

New combinations in Anthoxanthum (Poaceae) are provided for six endemic Aotearoa / New Zealand species earlier placed in Hierochloe: Anthoxanthum brunonis (Hook. f.) de Lange & C.J. James, comb. nov. (Hierochloe brunonis Hook. f.), A. cupreum (Zotov) de Lange & C.J. James, comb. nov. (H. cuprea Zotov), A. equisetum (Zotov) de Lange & C.J. James, comb. nov. (H. equiseta Zotov), A. fuscum (Zotov) de Lange & C.J. James, comb. nov. (H. fusca Zotov), A. novae-zelandiae Gand.) de Lange & C.J. James, comb. nov. (H. novae-zelandiae Gand.) and A. recurvatum (Hack.) de Lange & C.J. James, comb. nov. (H. fraseri Hook. f. var. recurvata Hack.). These six combinations were omitted from Schouten and Veldkamp (1985), in which the then recognised Hierochloe were transferred to Anthoxanthum and in subsequent treatments.
Subulicystidium perlongisporum (Trechisporales), the first record in Ukraine
Authors:   M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, 2 Tereshchenkivska Str., Kyiv 01601, Ukraine, Shevchenko M.V. , Zykova M.O. ,   M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, 2 Tereshchenkivska Str., Kyiv 01601, Ukraine
Published: September 2024

In this article we report the first record of Subulicystidium perlongisporum, a noteworthy corticioid fungus, which has never been previously recorded in Ukraine. A detailed morphological description of the examined specimen, along with the original photos, ecological specifics and global distribution details of the species are provided. The criteria for delimitation of long-spored species of Subulicystidium are discussed.
Artomyces microsporus (Auriscalpiaceae), a rare species that may be confused with A. pyxidatus
Authors:   M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Hayova V.
Published: September 2024

Artomyces microsporus, a lignicolous basidiomycete first reported in Europe from Ukraine almost two decades ago, is morphologically quite similar to A. pyxidatus, the most commonly encountered species of the genus across the temperate zone of the Northern Hemisphere. This survey is aimed at defining useful characters for the reliable distinction of these species in the field, in addition to species identification based on molecular data. Both morphological characters of the basidiomes and host/substrate preferences of A. microsporus are considered. The defined characters for identification of A. microsporus can be widely used by citizen scientists providing observations of the fungal occurrences for biodiversity repositories and social media networks. The reliable data for the species identification will contribute to determining the distribution patterns of A. microsporus regionally and worldwide.
Increasing germination and antioxidant activity of aged wheat and triticale grains by priming with gamma-aminobutyric acid
Authors:   Yuriev Institute of Plant Production, National Academy of Agrarian Sciences of Ukraine, 142 Heroiv Kharkova Ave., Kharkiv 61060, Ukraine, Shakhov I.V. , Kokorev A.I. ,   Yuriev Institute of Plant Production, National Academy of Agrarian Sciences of Ukraine, 142 Heroiv Kharkova Ave., Kharkiv 61060, Ukraine, Yastreb T.O. ,   Yuriev Institute of Plant Production, National Academy of Agrarian Sciences of Ukraine, 142 Heroiv Kharkova Ave., Kharkiv 61060, Ukraine, Dmitriev A.P. ,   Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, 148 Akademika Zabolotnogo Str., Kyiv 03143, Ukraine, Kolupaev Yu.E. ,   Yuriev Institute of Plant Production, National Academy of Agrarian Sciences of Ukraine, 142 Heroiv Kharkova Ave., Kharkiv 61060, Ukraine
Published: September 2024

During storage, elevated temperature and humidity cause accelerated aging and deterioration of seeds of various plant species, including important cultivated cereals, such as wheat and triticale. Germination of old seeds can be facilitated by seed priming with physiologically active substances that correct the pro/antioxidant balance and reduce the development of oxidative stress. Gamma-aminobutyric acid (GABA) is a regulatory compound with direct and indirect antioxidant effects. However, its effect on the germination of grains of cereals with low germination has not been extensively studied. The objective of this study was to examine the impact of GABA priming on the germination of aged grains of winter bread wheat (Triticum aestivum ‘Scorpion’) and winter triticale (×Triticosecale ‘Raritet’). the extent of oxidative stress and the state of the antioxidant system in seedlings. The results indicated that a three-hour treatment of grains with GABA at the optimal concentration (1 mM) resulted in a significant (18–21%) increase in germination energy and seed germination rate, as well as an increase in the biomass of shoots and roots of seedlings of both species. Concomitantly, the influence of GABA resulted in a reduction in oxidative stress markers, including the generation of superoxide anion radicals, hydrogen peroxide content, and the product of lipid peroxidation malondialdehyde. In wheat seedlings, the total content of phenolic compounds increased, while in triticale seedlings, the content of anthocyanins increased almost twofold. In seedlings derived from GABA-primed grains, catalase activity was also significantly elevated in the absence of notable alterations in superoxide dismutase and peroxidase activity. It was postulated that GABA priming is a promising approach for enhancing the germination of cereal seeds with diminished sowing quality.
Estrategias de acompañamiento familiar en la educación preescolar durante el contexto mexicano de la pandemia
Authors: Jiménez Ramírez Genis Yaisuri , Cortes Flores Aída , De Luna Jiménez Myriam
Published: September 2024

Las familias son los primeros agentes socializadores y promotores del aprendizaje en la primera infancia, por lo que es fundamental generar evidencia empírica sobre el impacto que tiene el acompañamiento educativo familiar en el desarrollo cognitivo, lingüístico y socioemocional en los primeros años de vida. De ahí que el objetivo de este estudio fue conocer las estrategias de acompañamiento familiar que favorecieron los procesos de enseñanza aprendizaje en el nivel preescolar, durante el contexto de la nueva normalidad originado por la pandemia. La investigación fue realizada entre los meses de mayo y julio del 2023. Participaron 28 familias que tenían una hija o hijo inscrito en el nivel preescolar de una escuela privada ubicada en la Ciudad de México durante el ciclo escolar 2021-2022. La investigación fue cualitativa con un enfoque fenomenológico, a partir del cual se realizó un análisis temático del discurso de las familias mediante la técnica de grupos focales y un cuestionario. Los resultados obtenidos permitieron la construcción de un sistema de categorías vinculado con el acompañamiento familiar, el cual comprendió las siguientes subcategorías: (1) estrategias didácticas y (2) estrategias de apoyo emocional. El sistema de acompañamiento familiar fue analizado desde un enfoque ecológico mediante el cual se identificaron las siguientes temáticas: (1) barreras para el acompañamiento familiar, (2) preocupaciones de la familia en torno al desarrollo de su hija o hijo, y (3) aprendizajes familiares sobre la nueva normalidad. Los resultados de investigación corroboran la importancia que tuvo el acompañamiento familiar en la trayectoria académica del estudiantado en el contexto de la pandemia; asimismo, resalta las estrategias educativas implementadas en casa y la capacidad de agencia familiar para afrontar las problemáticas socioemocionales y de ajuste escolar durante la emergencia sanitaria.
Scoping Review: Effectiveness of NDO-LID as new biomarker for leprosy diagnosis
Authors: Julianto Ricky , Putri Rachmah Diana
Published: September 2024

Leprosy is a chronic infectious disease that continues to be prevalent in over 120 countries worldwide, with over 200,000 cases of leprosy. The challenge in controlling leprosy cases is early diagnosis and identification of new cases. It is very important to diagnose quickly and precisely to prevent further disability due to nerve damage. This scoping review aims to assess the effectiveness of NDO-LID as a diagnostic test. The search strategy covered three main databases: PubMed, Science Direct, and Google Scholar, focusing on peer-reviewed studies published from 2019 to 2023. PRISMA guidelines were followed during the literature search. The keywords "NDO-LID" and "NDO-LID Leprosy" were used in the search to find relevant studies. The review included three articles out of 370 relevant ones. The review encompassed 1953 participants across three studies. Part of the review reported on 11 diagnostic test assessments. NDO-LID can detect leprosy patients in both MB and PB cases. Although NDO-LID has higher sensitivity and specificity values than its comparators, further studies are needed to test the accuracy of the diagnostic test more comprehensively to provide stronger and more convincing evidence.
The Association of PELOD-2 Score and Nutritional Status of Children Treated in the Pediatric Intensive Care Unit at Prof. Dr. I.G.N.G. Ngoerah Hospital Denpasar, 2020-2022
Authors: Putu Andrie Setiawan  , Wati Dyah Kanya , Suparyatha Ida Bagus Gde , Hartawan I Nyoman Budi , Sidiartha I Gusti Lanang , Pratiwi I Gusti Ayu Putu Eka
Published: September 2024

Background: The Pediatric Logistic Organ Dysfunction (PELOD-2) is used to measure the severity of vital organ dysfunction due to disease or other medical conditions in pediatric patients admitted to the Pediatric Intensive Care Unit (PICU). Nutritional status is a crucial factor affecting the body's ability to fight disease and the risk of complications. The aim was to evaluate the association between PELOD-2 scores and nutritional status in children treated in the PICU of Prof. Dr. I.G.N.G. Ngoerah Hospital Denpasar.
Methods: This study employed a retrospective cross-sectional design using medical record data of children aged 1 month to 18 years treated in the PICU of Prof. Dr. I.G.N.G. Ngoerah Hospital Denpasar from January 2020 to December 2022. Data were collected from 428 subjects meeting the inclusion and exclusion criteria. Data analysis was performed using Chi-Square tests and logistic regression.
Results: The study results indicated that PELOD-2 scores were significantly associated with poor nutritional status (OR: 1.71; 95% CI: 1.107–2.73), ventilator use (OR: 5.4; 95% CI: 3.336–8.913), and gender, with males being at higher risk (OR: 1.66; 95% CI: 1.042–2.654). Older age was found to be a protective factor against high PELOD-2 scores (ExpB: 0.996; 95% CI: 0.993–1.00).
Conclusion: PELOD-2 scores are significantly associated with poor nutritional status, age, ventilator use, and gender in children treated in the PICU.
Two-year follow-up of a post-traumatic choroidal rupture with a lens luxation patient: a case report
Authors: Cinthiadewi Made Dessy Gangga Ayu , Firmansjah Muhammad
Published: September 2024

Background: A choroidal rupture is a break in the choroid, Bruch membrane, and retinal pigment epithelium (RPE) that occurs most commonly as a result of blunt ocular trauma. It may be a secondary effect of direct or indirect trauma. This case illustrates an indirect post-traumatic choroidal rupture with lens luxation. Three months following the incident, the patient presented with impaired vision. The posterior segment was found to have a luxated lens during the objective examination of the eye. The funduscopy revealed a white, curvilinear, crescent-shaped streak that was concentric to the optic nerve.
Case description: A 45-year-old male from East Java visited the ophthalmology outpatient clinic with a chief complaint of blurred vision in the left eye for three months, followed by pain in the left eye. On examination, the visual acuity was a one-meter counting finger. The lens was luxated to the posterior. The funduscopy examination revealed a curvilinear crescent-shaped streak. Vitrectomy and lensectomy were implemented. After four months of aphakia, the patient underwent a scleral fixation intraocular lens (IOL) implantation. The visual acuity was satisfactory, and no complications were observed during the two-year follow-up.
Conclusion: We reported a post-traumatic choroidal rupture and a lens luxation with an optimal outcome after a two-year follow-up.
Devising a methodology for prototyping convex-concave parts using reverse-engineering technology providing the predefined geometric accuracy of their manufacturing
Authors: Maiorova Kateryna , Kapinus Oleksandra , Nikichanov Viacheslav , Skyba Oleksandr , Suslov Artem
Published: September 2024

The object of this paper is the geometric accuracy of the acquired portrait from the 3D printed full-scale sample in the reduced size of the convex-concave parts compared to the correspondingly reduced ideal one.
The subject of research is reverse engineering and additive technology for manufacturing convex-concave parts for mechanical engineering (ME). A new prototyping methodology for convex-concave parts of mechanical engineering objects has proposed. Underlying the methodology is the use of the scaled (by reducing in size) ideal portrait of the 3D scanned original part. The decision to start the production is made by comparing the geometry of the portrait acquired from the 3D printed sample in reduced size with the ideal one, provided that the values are within the tolerance range. The following results were obtained. A design and technological analysis of the blade of the pumped hydroelectric power station was performed, after which a 3D scanner and a 3D printer were selected. A 3D scan of the blade with the formation of a portrait in the STL format file was implemented, as well as its refinement into an ideal one. From the geometric features and shapes of the blade, as well as the technical characteristics of the 3D printer, the percentage of reducing the sample for printing (by 75 % of the original dimensions) was calculated. According to the rated dimensions of the original part and the reduced sample, the tolerance field was set for the size: 0.6 mm and 0.25 mm, respectively, at 12 quality of the part’s manufacturing accuracy. Inspection of the printed sample and comparison with the correspondingly reduced ideal portrait revealed a deviation from –0.123 to +0.120 mm, which is within the defined tolerance field for the manufactured reduced sample. The results of experimental studies confirmed the adequacy of the proposed methodology for prototyping the mechanical engineering parts and verified the theoretical foundations of reverse engineering for convex-concave parts of any large size by using a proportional reduction in the size of finished portraits