bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Five-Day vs. Fourteen-Day Incubation of Cultures for Diagnosis of Periprosthetic Joint Infection in Hip Arthroplasty
Authors: Baez Catalina , MacDonell Robert , Tishad Abtahi , Prieto Hernan A. , Miley Emilie N. , Deen Justin T. , Gray Chancellor F. , Parvataneni Hari K. , Pulido Luis
Published: July 2024

Background: Periprosthetic joint infections (PJI) are among the most morbid complications in total hip arthroplasty (THA). The ideal incubation time, however, for intraoperative cultures for PJI diagnosis remains unclear. As such, the aim of this study was to determine if any differences existed in culture-positive rates and organism detection between five-day and fourteen-day cultures. Methods: This retrospective cohort study consisted of THA cases diagnosed with PJI performed between May 2014 and May 2020 at a single tertiary-care institution. Analyses compared five-day and fourteen-day cultures and carried out a pre-specified subgroup analysis by organism and PJI type. Results: A total of 147 surgeries were performed in 101 patients (57.1% females), of which 65% (n = 98) obtained five-day cultures and 34% (n = 49) obtained fourteen-day cultures. The positive culture rate was 67.3% (n = 99) with Staphylococcus aureus being the most common pathogen identified (n = 41 specimens, 41.4%). The positive culture rate was not significantly different between groups (66.3% five-day, 69.4% fourteen-day, p = 0.852). Fourteen-day cultures had a significantly longer time-to-positive culture (5.0 days) than five-day cultures (3.0 days, p < 0.001), a higher rate of fungi (5.6% vs. 0%), and a lower rate of Gram-negatives (4.5% vs. 18.7%, p = 0.016). Conclusions: Fourteen-day cultures did not increase the positivity rate, had higher rates of slow-growth pathogens, and had a longer time-to-positivization than five-day cultures. Prolonged culture holds may provide more thorough organism detection for PJI without increasing the diagnostic culture yield.
Identification and Positioning Method of Bulk Cargo Terminal Unloading Hopper Based on Monocular Vision Three-Dimensional Measurement
Authors: Shen Ziyang , Wang Jiaqi , Zhang Yujie , Zheng Luocheng , Mi Chao , Shen Yang
Published: July 2024

Rapid identification and localization of dry bulk cargo hoppers are currently core issues in the automation control of gantry cranes at dry bulk terminals. The current conventional method relies on LiDAR systems for the identification and positioning of bulk unloading hoppers. However, this approach is complex and costly. In contrast, GPS-based positioning solutions for bulk unloading hoppers are prone to damage due to the vibrations generated during the operation process. Therefore, in this paper, a hopper localization system based on monocular camera vision is proposed to locate the position of the bulk unloading hopper. The hopper identification and localization process are divided into three stages. The first stage uses the improved YOLOv5 model to quickly and roughly locate the hopper target. The second stage uses morphological geometrical features to locate the corner points of the hopper target. The third stage determines the three-dimensional coordinates of the hopper target by solving the position of the corner points in the world coordinate system through the PnP (Perspective-n-Point) algorithm. The experimental results show that the average positioning accuracy of the coordinates of the method is above 93%, demonstrating the accuracy and effectiveness of the method.
Recurrent Echinococcosis Exhibiting Hydatoptysis: A Rare Case with Imaging Insights
Authors: Konstantinidou Maria , Eleftheriadou Eleni , Kamariotou Effimia , Rampiadou Christina , Papaemmanouil Styliani , Chloros Diamantis
Published: July 2024

Echinococcosis presents diverse clinical manifestations, including pulmonary hydatidosis, often asymptomatic but complicated by rare yet dramatic occurrences like hydatoptysis. Here, we report a unique case of recurrent pulmonary and abdominal hydatidosis in a 59-year-old female with bronchial fistulization and hydatoptysis, despite previous surgical interventions. Imaging revealed characteristic features aiding in diagnosis and management decisions. The challenges in managing recurrent echinococcosis underscore the importance of comprehensive follow-up and multidisciplinary care. Surgical intervention remains pivotal, supplemented by medical therapy with careful consideration of risks and benefits. This case also presents rare images, absent in much of the literature, which provide valuable insights into the disease’s presentation and progression.
New Biomarkers in Liver Fibrosis: A Pass through the Quicksand?
Authors: Tagliaferro Marzia , Marino Mariapaola , Basile Valerio , Pocino Krizia , Rapaccini Gian Ludovico , Ciasca Gabriele , Basile Umberto , Carnazzo Valeria
Published: July 2024

Chronic liver diseases (CLD) stem from various causes and lead to a gradual progression that ultimately may result in fibrosis and eventually cirrhosis. This process is typically prolonged and asymptomatic, characterized by the complex interplay among various cell types, signaling pathways, extracellular matrix components, and immune responses. With the prevalence of CLD increasing, diagnoses are often delayed, which leads to poor prognoses and in some cases, the need for liver transplants. Consequently, there is an urgent need for the development of novel, non-invasive methods for the diagnosis and monitoring of CLD. In this context, serum biomarkers—safer, repeatable, and more acceptable alternatives to tissue biopsies—are attracting significant research interest, although their clinical implementation is not yet widespread. This review summarizes the latest advancements in serum biomarkers for detecting hepatic fibrogenesis and advocates for concerted efforts to consolidate current knowledge, thereby providing patients with early, effective, and accessible diagnoses that facilitate personalized therapeutic strategies.
Precision Medicine in Peritoneal Dialysis: An Expert Opinion on the Application of the Sharesource Platform for the Remote Management of Patients
Authors: Neri Loris , Di Liberato Lorenzo , Alfano Gaetano , Allegrucci Valeria , Appio Nicoletta , Bussi Carla , Cannarile Daniela Cecilia , De Palma Ilaria , Di Stante Silvio , Pacifico Rosa , Panuccio Vincenzo , Porreca Silvia , Terlizzi Vincenzo , D’Alonzo Silvia , Viglino Giusto
Published: July 2024

The management of end-stage kidney disease (ESKD) has been constantly evolving over the last decade with the development of targeted approaches. In this field, telemedicine and remote monitoring are based on the availability of new cyclers that allow for bidirectional communication (between patient and physician) and for the application of the Sharesource cloud-based platform. These technologies allow patients with ESKD to undergo automated peritoneal dialysis (APD) at home. However, these approaches are not well standardized and largely applied yet. Therefore, this study aimed to elaborate a protocol for the utilization of the Sharesource platform to facilitate the practical management of patients treated with APD. A series of expert meetings were held between September 2022 and January 2023 in Italy. The participants (ten nephrologists and five nurses) from nine Italian public dialysis centers shared their opinions, examined the current scientific literature in the field, and reviewed the key characteristics of the Sharesource system to achieve a common position on this topic. A detailed and practical document containing experts’ opinions and suggestions on the use of the Sharesource platform for the management of patients treated with APD was produced. This expert opinion might represent a new useful instrument in clinical practice for managing patients undergoing home-based peritoneal dialysis (PD) through the Sharesource platform, which is valid not only for Italy. These recommendations pave the way to novel patient-centered and personalized therapeutic approaches for ESKD and highlight the advantages of telemedicine and remote monitoring in the management of patients with ESKD undergoing PD and its positive impact on their quality of life.
Assessment Accuracy of 2D vs. 3D Imaging for Custom-Made Acetabular Implants in Revision Hip Arthroplasty
Authors: Nees Timo Albert , Mueller Christian Thomas , Innmann Moritz Maximilian , Spranz David Maximilian , Westhauser Fabian , Renkawitz Tobias , Reiner Tobias , Walker Tilman
Published: July 2024

Revision total hip arthroplasty (rTHA) presents significant challenges, particularly in patients with severe acetabular bone defects. Traditional treatment options often fall short, leading to the emergence of custom-made 3D-printed acetabular implants. Accurate assessment of implant positioning is crucial for ensuring optimal postoperative outcomes and for providing feedback to the surgical team. This single-center, retrospective cohort study evaluates the accuracy of standard 2D radiographs versus 3D CT scans in assessing the positioning of these implants, aiming to determine if 2D imaging could serve as a viable alternative for the postoperative evaluation. We analyzed the implant positions of seven rTHA patients with severe acetabular defects (Paprosky ≥ Type IIIA) using an alignment technique that integrates postoperative 2D radiographs with preoperative CT plans. Two independent investigators, one inexperienced and one experienced, measured the positioning accuracy with both imaging modalities. Measurements included translational shifts from the preoperatively templated implant position in the craniocaudal (CC), lateromedial (LM), and ventrodorsal (VD) directions, as well as rotational differences in anteversion (AV) and inclination (INCL). The study demonstrated that 2D radiographs, when aligned with preoperative CT data, could accurately assess implant positions with precision nearly comparable to that of 3D CT scans. Observed deviations were 1.4 mm and 2.7 mm in CC and LM directions, respectively, and 3.6° in AV and 0.7° in INCL using 2D imaging, all within clinically acceptable ranges. For 3D CT assessments, mean interobserver variability was up to 0.9 mm for translational shifts and 1.4° for rotation, while for 2D alignment, observer differences were 1.4 mm and 3.2° for translation and rotation, respectively. Comparative analysis of mean results from both investigators, across all dimensions (CC, LM, AV, and INCL) for 2D and 3D matching, showed no significant differences. In conclusion, conventional anteroposterior 2D radiographs of the pelvis can sufficiently determine the positioning of custom-made acetabular implants in rTHA. This suggests that 2D radiography is a viable alternative to 3D CT scans, potentially enhancing the implementation and quality control of advanced implant technologies.
Development of a User-Friendly Self-Screening Tool for Assessing Metabolic Syndrome Risk in Youths from Economically Challenged Regions
Authors: Xavier Jacqueline Fernandes de Sa , Feuerstein Shirley C. , De Moraes Augusto Cesar Ferreira , de Oliveira Tiago Almeida , da Silva Gomes Evellyn Ravena , de Almeida Silva Maria Isabela Alves , de Oliveira Luiz Fernando , de Carvalho Heraclito Barbosa , Marin Kliver Antonio , Nascimento-Ferreira Marcus Vinicius
Published: July 2024

Background: Metabolic syndrome increases the risk of heart disease and diabetes. Early identification and management are crucial, especially in economically challenged regions with limited healthcare access. Aims: To develop nomograms for individualized risk estimation for metabolic syndrome in young people from low-income regions. Methods: We assessed 496 college students from two Brazilian cities with Gini indices ≤0.56. Of these, 69.9% were female, 65.1% were younger than 20 years, 71.8% were non-white, and 64.3% were enrolled in health-related courses. For external validity, we assessed metabolic syndrome in a subset of 375 students. Results: We found 10 variables associated with abdominal obesity by logistic regression: age, biological sex, physical education facilities, enrollment in sports competitions during elementary school, grade retention, physical education as the preferred subject, physical education classes per week, and enrollment in sports training in secondary school (score A); adherence to 24 h movement behaviors (B score); and body weight (score C). We designed three nomograms (for scores A, B, and C), all of which showed acceptable performance according to the area under the receiver operating characteristic curve (≥0.70) and calibration (Hosmer–Lemeshow test, p > 0.05). In the external validation, we observed higher predictive capability for the A and B scores, while the C score had lower but still acceptable predictive ability. Conclusions: User-friendly self-reported data accurately predict metabolic syndrome among youths from economically challenging areas.
Nutrient Resorption in Young Stands of Three Native Tree Species to Support Restoration of Degraded Tropical Peatland in Indonesia
Authors: Junaedi Ahmad , Pribadi Avry , Mindawati Nina , Dharmawan I Wayan Susi , Octavia Dona , Kurniawan Hery , Fauzi Ridwan , Siahaan Hengki , Premono Bambang Tejo , Nugroho Ardiyanto Wahyu , Lisnawati Yunita , Yulianti  , Nurhayati Ati Dwi , Iqbal Mohamad
Published: July 2024

Nutrient resorption (NR) is a critical ecological process in forest ecosystems. However, there is a lack of knowledge about this process in the peatlands of Indonesia, and this may be seen as a research gap. In the present study, NR in young trees of three native species (Macaranga pruinosa, Cratoxylum arborescens, and Macaranga gigantea) and one exotic species (Acacia crassicarpa) in a drained tropical peatland was investigated. This study was conducted at an experimental plot in Pelalawan-Riau, Indonesia. Nutrient resorption efficiency (RE) and proficiency (RP) were calculated and correlated with soil properties, foliar nutrients, and growth variables. Our results revealed that M. pruinosa exhibited an RE value for phosphorus (PRE) that was 64% higher than that for the second-ranked native species but still significantly (84%) lower than that for A. crassicarpa. RE values for nitrogen (NRE) and potassium (KRE) did not differ significantly among species, ranging from 39 to 42% and 41 to 56%, respectively, for native species, with figures of 45% and 66%, respectively, for A. crassicarpa. Finally, PRE exhibited strong and significant correlations with PRP and tree growth, a finding that indicated that the uptake and conservation of P nutrients are essential for the fitness of the three native species. Overall, the results of the present study may be seen as beneficial for species selection and the management of nutrients by those engaged in restoration of tropical peatland forests.
Overcoming Barriers and Fostering Adoption: Evaluating the Institutional Mainstreaming of Nature-Based Solutions in the Emilia-Romagna Region’s Socio-Ecological System
Authors: Carlone Teresa , Mannocchi Matteo
Published: July 2024

International organizations like the EU and IUCN are advocating for nature-based solutions (NBSs) as green alternatives for climate change adaptation and mitigation, especially in disaster risk reduction and urban planning. The H2020 OPERANDUM project was designed to address the major hydro-meteorological risks (floods, droughts, landslides, storm surge, and coastal erosions) through the deployment and assessment of NBSs in different contexts and areas affected by specific hazards. Despite growing research and funding, NBSs are still in the early stages of mainstream adoption and face challenges in acceptance and dissemination. Although designed to benefit both social and ecological systems, they remain a niche area with low perceived effectiveness among technicians and decision-makers. Their uptake requires a paradigm shift that includes a change in cultural-cognitive institutions, a different and wider set of knowledge than traditional engineering (ecological, social), and an adaptive management approach, missing within the current governance system. Using a qualitative case study research method, this paper aims to identify barriers in mainstreaming NBSs for DRR (disaster risk reduction) in the Emilia-Romagna region—influenced not only by individual beliefs but also by variables tied to technical culture and local procedural norms—and emphasizing the importance of combining social and ecological indicators in socio-ecological system analysis.
Nanoindentation Creep Behavior of Additively Manufactured H13 Steel by Utilizing Selective Laser Melting Technology
Authors: Giarmas Evangelos , Tzimtzimis Emmanouil K. , Kladovasilakis Nikolaos , Tzovaras Dimitrios , Tzetzis Dimitrios
Published: July 2024

Nowadays, H13 hot work steel is a commonly used hot work die material in the industry; however, its creep behavior for additively manufactured H13 steel parts has not been widely investigated. This research paper examines the impact of volumetric energy density (VED), a critical parameter in additive manufacturing (AM), and the effect of post heat-treatment nitrification on the creep behavior of H13 hot work tool steel, which is constructed through selective laser melting (SLM), which is a powder bed fusion process according to ISO/ASTM 52900:2021. The study utilizes nanoindentation tests to investigate the creep response and the associated parameters such as the steady-state creep strain rate. Measurements and observations taken during the holding phase offer a valuable understanding of the behavior of the studied material. The findings of this study highlight a substantial influence of both VED and nitrification on several factors including hardness, modulus of elasticity, indentation depth, and creep displacement. Interestingly, the creep strain rate appears to be largely unaltered by these parameters. The study concludes with the observation that the creep stress exponent (n) shows a decreasing trend with an increase in VED and the application of nitrification treatment.
Synthesis of Keratin Nanoparticles Extracted from Human Hair through Hydrolysis with Concentrated Sulfuric Acid: Characterization and Cytotoxicity
Authors: Silva Otavio A. , Rossin Ariane R. S. , Lima Antônia M. de Oliveira , Valente Andressa D. , Garcia Francielle P. , Nakamura Celso V. , Follmann Heveline D. M. , Silva Rafael , Martins Alessandro F.
Published: July 2024

Human hair, composed primarily of keratin, represents a sustainable waste material suitable for various applications. Synthesizing keratin nanoparticles (KNPs) from human hair for biomedical uses is particularly attractive due to their biocompatibility. In this study, keratin was extracted from human hair using concentrated sulfuric acid as the hydrolysis agent for the first time. This process yielded KNPs in both the supernatant (KNPs-S) and precipitate (KNPs-P) phases. Characterization involved scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), Zeta potential analysis, X-ray diffraction (XRD), and thermogravimetric analysis (TG). KNPs-S and KNPs-P exhibited average diameters of 72 ± 5 nm and 27 ± 5 nm, respectively. The hydrolysis process induced a structural rearrangement favoring β-sheet structures over α-helices in the KNPs. These nanoparticles demonstrated negative Zeta potentials across the pH spectrum. KNPs-S showed higher cytotoxicity (CC50 = 176.67 µg/mL) and hemolytic activity, likely due to their smaller size compared to KNPs-P (CC50 = 246.21 µg/mL), particularly at concentrations of 500 and 1000 µg/mL. In contrast, KNPs-P did not exhibit hemolytic activity within the tested concentration range of 32.5 to 1000 µg/mL. Both KNPs demonstrated cytocompatibility with fibroblast cells in a dose-dependent manner. Compared to other methods reported in the literature and despite requiring careful washing and neutralization steps, sulfuric acid hydrolysis proved effective, rapid, and feasible for producing cytocompatible KNPs (biomaterials) in single-step synthesis.
Preparation and Property Analysis of Antibacterial Fiber Membranes Based on Hyperbranched Polymer Quaternary Ammonium Salts
Authors: Zhu Jiehui , Guo Ying , Yao Lirong , Pan Gangwei , Zhang Desuo , Yang Jianwei
Published: July 2024

Due to their excellent properties, antimicrobial fiber membranes are widely applied in bioprotective materials. This work addresses the preparation of thermoplastic polyurethane (TPU)-based fiber membranes with active antimicrobial properties. 2-hydroxypropyl trimethyl ammonium chloride-terminated hyperbranched polymer (HBP-HTC) was synthesized and used as an antimicrobial agent. The fiber membranes were obtained by electrospinning a mixed solution of HBP-HTC and TPU. Different electrospinning conditions were investigated, such as the spinning voltage and drum rotation speed. The fiber membrane prepared under a 22 kV anode voltage and 100 rpm rotation speed had an average fiber diameter of 1.66 μm with a concentrated diameter distribution. Antibacterial tests showed that when the fiber membrane was loaded with 1500 mg/kg of HBP-HTC, the antibacterial rates of E. coli as well as S. aureus both reached 99.99%, exhibiting excellent proactive antimicrobial performance. Moreover, the protective performance of the fiber membrane was outstanding, with a filtration efficiency of 99.9%, a hydrostatic pressure resistance greater than 16,758 Pa, and a moisture permeability of 2711.0 g⋅(m2⋅d)−1.
Comparative Analysis of Coatings Applied for Anti-Corrosion Protection of Public Transport Vehicles’ Structural Parts
Authors: Skotnicki Wojciech , Jędrzejczyk Dariusz
Published: July 2024

The conducted research focused on anti-corrosion systems applied for the protection of structural parts used in public transport vehicles. Detailed tests were carried out on samples taken from the brackets supporting the doors of a public transport bus. This work includes the results of the chemical analysis of the composition of snow–mud samples taken from the selected bus route and the results of laboratory tests performed on samples with various anti-corrosion coatings. Four types of samples made of S235JR steel with a zinc coating deposited by thermo-diffusion, electroplating, hot-dip zinc galvanization, and the cataphoresis method were tested. Both non-destructive tests—NDTs (the measurement of coating thickness and roughness, microscopic observations)—and destructive tests—DTs (scratch tests, salt chamber tests)—were performed. The conducted tests proved that the most effective method is the use of anti-corrosive hot-dip zinc coating.
MambaSR: Arbitrary-Scale Super-Resolution Integrating Mamba with Fast Fourier Convolution Blocks
Authors: Yan Jin , Chen Zongren , Pei Zhiyuan , Lu Xiaoping , Zheng Hua
Published: July 2024

Traditional single image super-resolution (SISR) methods, which focus on integer scale super-resolution, often require separate training for each scale factor, leading to increased computational resource consumption. In this paper, we propose MambaSR, a novel arbitrary-scale super-resolution approach integrating Mamba with Fast Fourier Convolution Blocks. MambaSR leverages the strengths of the Mamba state-space model to extract long-range dependencies. In addition, Fast Fourier Convolution Blocks are proposed to capture the global information in the frequency domain. The experimental results demonstrate that MambaSR achieves superior performance compared to different methods across various benchmark datasets. Specifically, on the Urban100 dataset, MambaSR outperforms MetaSR by 0.93 dB in PSNR and 0.0203 dB in SSIM, and on the Manga109 dataset, it achieves an average PSNR improvement of 1.00 dB and an SSIM improvement of 0.0093 dB. These results highlight the efficacy of MambaSR in enhancing image quality for arbitrary-scale super-resolution.
A Linear Composition Operator on the Bloch Space
Authors: Zhu Xiangling , Hu Qinghua
Published: July 2024

Let n∈N0, ψ be an analytic self-map on D and u be an analytic function on D. The single operator Du,ψn acting on various spaces of analytic functions has been a subject of investigation for many years. It is defined as (Du,ψnf)(z)=u(z)f(n)(ψ(z)),f∈H(D). However, the study of the operator Pu→,ψk, which represents a finite sum of these operators with varying orders, remains incomplete. The boundedness, compactness and essential norm of the operator Pu→,ψk on the Bloch space are investigated in this paper, and several characterizations for these properties are provided.
New Random Walk Algorithm Based on Different Seed Nodes for Community Detection
Authors: Cai Jiansheng , Li Wencong , Zhang Xiaodong , Wang Jihui
Published: July 2024

A complex network is an abstract modeling of complex systems in the real world, which plays an important role in analyzing the function of complex systems. Community detection is an important tool for analyzing network structure. In this paper, we propose a new community detection algorithm (RWBS) based on different seed nodes which aims to understand the community structure of the network, which provides a new idea for the allocation of resources in the network. RWBS provides a new centrality metric (MC) to calculate node importance, which calculates the ranking of nodes as seed nodes. Furthermore, two algorithms are proposed for determining seed nodes on networks with and without ground truth, respectively. We set the number of steps for the random walk to six according to the six degrees of separation theory to reduce the running time of the algorithm. Since some traditional community detection algorithms may detect smaller communities, e.g., two nodes become one community, this may make the resource allocation unreasonable. Therefore, modularity (Q) is chosen as the optimization function to combine communities, which can improve the quality of detected communities. Final experimental results on real-world and synthetic networks show that the RWBS algorithm can effectively detect communities.
Hyperpolyadic Structures
Authors: Duplij Steven
Published: July 2024

We introduce a new class of division algebras, the hyperpolyadic algebras, which correspond to the binary division algebras R, C, H, O without considering new elements. First, we use the matrix polyadization procedure proposed earlier which increases the dimension of the algebra. The algebras obtained in this way obey binary addition and a nonderived n-ary multiplication and their subalgebras are division n-ary algebras. For each invertible element, we define a new norm which is polyadically multiplicative, and the corresponding map is a n-ary homomorphism. We define a polyadic analog of the Cayley–Dickson construction which corresponds to the consequent embedding of monomial matrices from the polyadization procedure. We then obtain another series of n-ary algebras corresponding to the binary division algebras which have a higher dimension, which is proportional to the intermediate arities, and which are not isomorphic to those obtained by the previous constructions. Second, a new polyadic product of vectors in any vector space is defined, which is consistent with the polyadization procedure using vectorization. Endowed with this introduced product, the vector space becomes a polyadic algebra which is a division algebra under some invertibility conditions, and its structure constants are computed. Third, we propose a new iterative process (we call it the “imaginary tower”), which leads to nonunital nonderived ternary division algebras of half the dimension, which we call “half-quaternions” and “half-octonions”. The latter are not the subalgebras of the binary division algebras, but subsets only, since they have different arity. Nevertheless, they are actually ternary division algebras, because they allow division, and their nonzero elements are invertible. From the multiplicativity of the introduced “half-quaternion” norm, we obtain the ternary analog of the sum of two squares identity. We show that the ternary division algebra of imaginary “half-octonions” is unitless and totally associative.
Combined Statistical Analysis of Glioblastoma Outcomes—A Neurosurgical Single-Institution Retrospective Study
Authors: Tataranu Ligia Gabriela , Staicu Georgiana Adeline , Dricu Anica , Turliuc Serban , Paunescu Dan , Kamel Amira , Rizea Radu Eugen
Published: July 2024

Background and Objectives: Notwithstanding the major progress in the management of cancerous diseases in the last few decades, glioblastoma (GBM) remains the most aggressive brain malignancy, with a dismal prognosis, mainly due to treatment resistance and tumoral recurrence. In order to diagnose this disease and establish the optimal therapeutic approach to it, a standard tissue biopsy or a liquid biopsy can be performed, although the latter is currently less common. To date, both tissue and liquid biopsy have yielded numerous biomarkers that predict the evolution and response to treatment in GBM. However, despite all such efforts, GBM has the shortest recorded survival rates of all the primary brain malignancies. Materials and Methods: We retrospectively reviewed patients with a confirmed histopathological diagnosis of glioblastoma between June 2011 and June 2023. All the patients were treated in the Third Neurosurgical Department of the Clinical Emergency Hospital “Bagdasar-Arseni” in Bucharest, and their outcomes were analyzed and presented accordingly. Results: Out of 518 patients in our study, 222 (42.8%) were women and 296 (57.14%) were men. The most common clinical manifestations were headaches and limb paralysis, while the most frequent tumor locations were the frontal and temporal lobes. The survival rates were prolonged in patients younger than 60 years of age, in patients with gross total tumoral resection and less than 30% tumoral necrosis, as well as in those who underwent adjuvant radiotherapy. Conclusions: Despite significant advancements in relation to cancer diseases, GBM is still a field of great interest for research and in great need of new therapeutic approaches. Although the multimodal therapeutic approach can improve the prognosis, the survival rates are still short and the recurrences are constant.
The Efficacy of Paraprobiotic Lozenges (Lactobacillus helveticus MIMLh5) for the Prevention of Acute and Chronic Nose and Throat Infections in Children
Authors: Baljošević Ivan , Šubarević Vladan , Stanković Katarina , Bajec Opančina Aleksandra , Novković Mladen , Petrovic Masa
Published: July 2024

Background and Objectives: Tonsillitis is common in children and is predominantly caused by viruses and, less frequently, by bacteria such as group A beta-hemolytic streptococcus. The treatment primarily involves supportive care; however, the overuse of antibiotics remains a concern due to rising antibiotic resistance. Probiotics, particularly Lactobacillus strains, have been shown to modulate immune responses, offering a potential alternative treatment. Materials and Methods: Our prospective single-arm, open-label study included 98 pediatric patients aged 5–15 years with recurrent throat and tonsil infections, from October 2022 to January 2023. Patients received lozenges containing heat-killed Lactobacillus helveticus MIMLh5. Monthly follow-ups involved a comprehensive ear, nose, and throat examination, throat cultures, and recording the frequency of infections and antibiotic use. Data were analyzed using SPSS 29.0, with statistical significance set at p < 0.05. Results: After three months, significant reductions were observed in the occurrences of nose and throat infections (p < 0.001), enlarged submandibular glands (p < 0.001), and positive throat cultures (p < 0.001). Antibiotic and corticosteroid prescriptions also significantly decreased (p < 0.001). Among children aged 5–10 years, significant improvements were noted in throat and tonsil infections (p < 0.001) and positive throat cultures (p = 0.012). Overall, there was a substantial reduction in school days missed (p < 0.001). Conclusions: The use of paraprobiotic Lactobacillus helveticus MIMLh5 lozenges significantly reduced the incidence of recurrent throat and tonsil infections in children, decreased the need for antibiotics and corticosteroids, and improved overall clinical outcomes without adverse effects. These findings support the use of paraprobiotic supplements as a safe and effective preventive measure for pediatric throat and tonsil infections.
Addressing Challenges in Chlamydia trachomatis Detection: A Comparative Review of Diagnostic Methods
Authors: Rodrigues Rafaela , Silva Ana Rita , Sousa Carlos , Vale Nuno
Published: July 2024

Chlamydial infections are one of the most common sexually transmitted bacterial infections worldwide, which is related to serious consequences for the mental, sexual, and reproductive health of women and men. The infection is commonly asymptomatic; consequently, screening programs for infection control have been introduced in some countries. The detection methods of Chlamydia trachomatis infections have evolved since the establishment of the first gold-standard detection method in the 1970s, the culture assay. Over the decades, many efforts were made to find methods with a higher sensitivity, until the 1990s, when, as a result of advances in molecular biology, nucleic acid amplification tests came into use with more sensitivity, and, currently, there are several available with which to detect infection. Therefore, herein, we will review the main methods used for CT detection and the differences between them, in terms of targets, infections that can be detected, sensitivity, and specificity. We will focus on some of the FDA-approved CT detection tests and highlight the major advantages and superiority of using molecular biology techniques. In addition, we will examine the larger challenges and limitations of the methods currently in use and discuss how they might be surpassed. Moreover, in this review, we will describe the next step to carry out after testing positive for CT infection.
Thin Amelanotic and Hypomelanotic Melanoma: Clinicopathological and Dermoscopic Features
Authors: Paolino Giovanni , Pampena Riccardo , Di Ciaccio Sofia Maria , Carugno Andrea , Cantisani Carmen , Di Nicola Matteo Riccardo , Losco Luigi , Bortone Giulio , Mercuri Santo Raffaele , Costanzo Antonio , Ardigò Marco , Valenti Mario
Published: July 2024

Background and Objectives: Amelanotic/hypomelanotic melanomas (AHMs) account for 2–8% of all cutaneous melanomas. Due to their clinical appearance and the lack of specific dermoscopic indicators, AHMs are challenging to diagnose, particularly in thinner cutaneous lesions. The aim of our study was to evaluate the clinicopathological and dermoscopic features of thin AHMs. Identifying the baseline clinical–pathological features and dermoscopic aspects of thin AHMs is crucial to better understand this entity. Materials and Methods: We divided the AHM cohort into two groups based on Breslow thickness: thin (≤1.00 mm) and thick (>1.00 mm). This stratification helped identify any significant clinicopathological differences between the groups. For dermoscopic analysis, we employed the “pattern analysis” approach, which involves a simultaneous and subjective assessment of different criteria. Results: Out of the 2.800 melanomas analyzed for Breslow thickness, 153 were identified as AHMs. Among these, 65 patients presented with thin AHMs and 88 with thick AHMs. Red hair color and phototype II were more prevalent in patients with thin AHMs. The trunk was the most common anatomic site for thin AHMs. Patients with thin AHMs showed a higher number of multiple melanomas. Dermoscopic analysis revealed no significant difference between thin AHMs and thick AHMs, except for a more frequent occurrence of residual reticulum in thin AHMs. Conclusions: Thin AHMs typically affect individuals with lower phototypes and red hair color. These aspects can be related to the higher presence of pheomelanin, which provides limited protection against sun damage. This also correlates with the fact that the trunk, a site commonly exposed to intermittent sun exposure, is the primary anatomical location for thin AHMs. Multiple primary melanomas are more common in patients with thin AHMs, likely due to an intrinsic predisposition as well as greater periodic dermatologic follow-ups in this class of patients. Apart from the presence of residual reticulum, no other significant dermoscopic differences were observed, complicating the differential diagnosis between thin and thick AHMs based on dermoscopy alone.
A Concise Guide to D-Wave Monitoring during Intramedullary Spinal Cord Tumour Surgery
Authors: Zurita Perea Santos Nicolás , Alvarez Abut Pablo Abel , Seidel Kathleen
Published: July 2024

D-waves (also called direct waves) result from the direct activation of fast-conducting, thickly myelinated corticospinal tract (CST) fibres after a single electrical stimulus. During intraoperative neurophysiological monitoring, D-waves are used to assess the long-term motor outcomes of patients undergoing surgery for intramedullary spinal cord tumours, selected cases of intradural extramedullary tumours and surgery for syringomyelia. In the present manuscript, we discuss D-wave monitoring and its role as a tool for monitoring the CST during spinal cord surgery. We describe the neurophysiological background and provide some recommendations for recording and stimulation, as well as possible future perspectives. Further, we introduce the concept of anti D-wave and present an illustrative case with successful recordings.
Identification of Plant Compounds with Mass Spectrometry Imaging (MSI)
Authors: García-Rojas Nancy Shyrley , Sierra-Álvarez Carlos Daniel , Ramos-Aboites Hilda E. , Moreno-Pedraza Abigail , Winkler Robert
Published: July 2024

The presence and localization of plant metabolites are indicative of physiological processes, e.g., under biotic and abiotic stress conditions. Further, the chemical composition of plant parts is related to their quality as food or for medicinal applications. Mass spectrometry imaging (MSI) has become a popular analytical technique for exploring and visualizing the spatial distribution of plant molecules within a tissue. This review provides a summary of mass spectrometry methods used for mapping and identifying metabolites in plant tissues. We present the benefits and the disadvantages of both vacuum and ambient ionization methods, considering direct and indirect approaches. Finally, we discuss the current limitations in annotating and identifying molecules and perspectives for future investigations.
Lactobacillus crispatus-Mediated Gut–Reproductive Tract Axis-Alleviated Microbial Dysbiosis and Oviductal Inflammation in a Laying Hen Model
Authors: Li Shinuo , Wang Qingfeng , Mi Jinqiu , Chen Haotian , Yuan Tianhao , Wang Yue , Zhao Lihong , Ma Qiugang , Huang Shimeng
Published: July 2024

Oviductal inflammation (OI) significantly reduces the egg production and economic returns in poultry farming. While Lactobacillus crispatus (LAC) is effective against inflammation, its role in treating or preventing oviductal inflammation is understudied. In this study, we investigated the therapeutic mechanisms of LAC on oviductal inflammation, with a focus on reproductive tract health, microbiome, gene expression, and cytokine levels. This study involved 24 Jingfen No. 6 laying hens aged 60 weeks, divided into four groups: the CON, OI, OI + LAC, and OI + heat-killed Lactobacillus crispatus (HLAC) groups. And it included a 10-day adaptation, a 7-day period for the development of OI using inflammation-inducing drugs (the control received saline), followed by an 8-day treatment in which the CON and OI groups received 1 mL of MRS broth daily, and the OI + LAC and OI + HLAC groups were treated with live and heat-killed Lactobacillus crispatus (109 CFUs/mL), respectively, with six hens in each group. This study showed that Lactobacillus crispatus supplementation significantly reduced the oviductal inflammation and atrophy in the hens, with the affected hens showing markedly lower egg production rates (p < 0.001) compared to the control and treated groups (OI + HLAC and OI + LAC). The daily intake of fresh (OI + LAC, p = 0.076) or heat-killed (OI + HLAC, p < 0.01) Lactobacillus crispatus notably enhanced the feed conversion efficiency. The OI group suffered significant ovarian damage and vascular rupture, more so than the CON group, while Lactobacillus crispatus supplementation mitigated this damage. The IL-1β, IL-6, and IL-8 levels were significantly elevated in the OI group compared to those in the OI + LAC group (p < 0.05), with a significant reduction in the TNF-α levels in the latter (p < 0.001). The supplementation improved the microbial composition in the cecum, isthmus, and shell gland, enriching the cecum with beneficial bacteria, such as Ruminococcus_torques_group and Megamonas. This approach fostered ovarian health and follicle differentiation and preserved the epithelial cell barrier function in the shell gland, reducing inflammatory damage in the genital tract. This dual efficacy underscores the role of the probiotic in diminishing oviductal inflammation, regardless of its state.
Optimization of Discrete Element Method Model to Obtain Stable and Reliable Numerical Results of Mechanical Response of Granular Materials
Authors: Zhang Yuyu , Li Li
Published: July 2024

The discrete element method (DEM) is largely used to simulate the geotechnical behavior of granular materials. However, numerical modeling with this type of code is expensive and time consuming, especially when fine particles are involved. This leads researchers to make use of different approaches to shorten the time of calculation without verifying the stability and reliability of numerical results, even though a compromise between the time of calculation and accuracy is commonly claimed. The particle size distribution (PSD) curve of studied granular material is completely ignored or arbitrarily cut. It is unclear if the ensued numerical results are still representative of the studied granular materials. Additionally, one can see a large number of numerical models established on a basis of calibration by ignoring the physical meaning and even measured values of some model parameters. The representativeness and reliability of the obtained numerical results are questionable. All these partly contribute to reducing the public’s confidence in numerical modeling. In this study, a methodology is illustrated to obtain an optimal DEM model, which minimizes the time of calculation and ensures stable and reliable numerical results for the mechanical behavior of a waste rock. The results indicate that the PSD curve of the studied waste rock can indeed be cut by excluding a portion of fine particles, while the Young’s modulus of the waste rock particles can also be decreased to accelerate the numerical calculations. A physical explanation of why the time of calculation can be shortened by reducing the Young’s modulus of waste rock particles is provided for the first time. Overall, the PSD cut, reduction in Young’s modulus, and time step must be determined through sensitivity analyses to ensure stable and reliable results with the shortest time of calculation. In addition, it is important to minimize the number of model parameters determined through the process of calibration, especially for those having physical meanings. In this study, the only model parameter having a clear physical meaning but difficult to measure is the rolling resistance coefficient for repose angle tests on the studied waste rock. Its value has to be obtained through a process of calibration against some experimental results. The validity and predictability of the calibrated numerical model have been successfully verified against additional experimental results.
Editorial for Special Issue “Rare Metal Ore Formations and Rare Metal Metallogeny”
Authors: Li Peng , Li Jiankang
Published: July 2024

Rare metals (usually defined as including elements such as Li, Rb, Cs, Be, Sn, Nb, Ta, Zr, Y, U, and rare-earth elements) are included in the list of “critical mineral resources” by major economies around the world [...]
Geochemistry of Middle Jurassic Coals from the Dananhu Mine, Xinjiang: Emphasis on Sediment Source and Control Factors of Critical Metals
Authors: Wang Ruoyu , Wang Wenfeng , Lu Qingfeng , Zhang Jiaming , Wang Wenlong , Dong Lingling
Published: July 2024

In recent years, coal-type critical metal deposits have become a research hotspot in coal geology. As a major coal-accumulating basin in the Xinjiang area, the Turpan-Hami Basin contains abundant coal resources and has the potential to become a large coal-type critical metal deposit. However, previous studies on the enrichment characteristics of critical metal elements in coal are few and need further research. Based on SEM-EDS, XRF, and ICP-MS experiments, this study investigates the coal petrology, mineralogy, and geochemistry of the No. 22 coal of the Xishanyao Formation from the Dananhu Coal Mine, Xinjiang, to identify the sediment source, depositional environment, and controlling factors of the critical metal elements of the No. 22 coal. The results showed that the Dananhu coals are characterized by a low ash yield, low total sulfur content, high volatile yield, and high inertinite proportions. Quartz, kaolinite, and illite are the main minerals in the coal. Compared with the world’s low-rank coals, Ni, Co, Mo, and Ta are slightly enriched, Li, Rb, Cs, Ba, Tl, Bi, and Ge are depleted, and the concentrations of other trace elements are comparable to the average values of the world’s low-rank coals. The REY of the Dannanhu coals exhibited high fractionation, with its enrichment patterns characterized by the H-type and M-H-type. Although most of the critical metals are not enriched in the Dannanhu coals, the Ga, Zr (Hf), and Nb (Ta) concentrations in the coal ash of the Dannanhu coals have reached the economic cut-off grade and have the potential to be a substitute for rare metal resources. The terrigenous detrital sources of the Dannanhu coals mainly come from the Paleozoic dacite, andesite, and a small amount of granite from the Harik Mountain and Eastern Bogda Mountain in the Turpan-Hami Basin. The Dannanhu coals are generally in a dry and hot depositional environment, with high salinity and weak reduction-oxidation. The low source input and weak reduction-oxidation environment have resulted in low concentrations of critical metal of the No. 22 coal from the Dananhu Coal Mine.