bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated Bioinformatic Analysis Reveals NOS2 as a Novel Ferroptosis-related Biomarker for Pre-eclampsia
Authors: Cai Shuangming , Huang Shan , Zhang Wenni , Xiao Huanshun , Yu Danfeng , Zhong Xuan , Tao Pei , Luo Yiping
Published: January 2023

Background Pre-eclampsia (PE) is a common condition in pregnancy; however, methods for early diagnosis and effective treatment options are lacking. Ferroptosis is a newly identified iron-dependent cell death pathway. The aim of this study was to investigate the role of ferroptosis-related genes in PE, the underlying mechanism, and their potential diagnostic value using a bioinformatics approach. Methods We downloaded the GSE48424 and GSE98224 datasets from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between PE and healthy pregnancy samples were identified in the GSE48424 dataset and subjected to weighted gene co-expression network analysis; the most relevant modules were intersected with known ferroptosis-related genes to distinctly identify the role of ferroptosis in PE. We further searched transcription factors and microRNAs that are predicted to regulate these ferroptosis-related genes, and patients in the GSE48424 dataset were divided into two groups according to high or low expression of the key ferroptosis-related genes associated with PE. To obtain robust key ferroptosis-related genes in PE, we validated their expression levels in the external dataset GSE98224. Finally, we performed a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay of these genes to evaluate their expression in the placenta samples of patients with PE and normal pregnancy. Results The most relevant module of PE in the GSE48424 dataset comprising the 565 identified DEGs contained a total of 3661 genes. After overlapping, we obtained six ferroptosis-related genes involved in PE. Among these genes, patients with PE displaying lower expression levels ofNOS2and higher expression levels ofPTGS2had a higher ferroptosis potential index. The expression pattern ofNOS2was consistent in the GSE48424 and GSE98224 datasets. RT-qPCR data confirmed thatNOS2expression was more significantly elevated in patients with PE than in those with a normal pregnancy. Conclusions Our study explored the diagnostic value of ferroptosis-related genes in PE, and identifiedNOS2as the key gene linking ferroptosis and PE, suggesting a new candidate biomarker for early PE diagnosis.
Family-centered empowerment program on self-efficacy, quality of life and daily activity in patients with COVID-19
Authors: Heidari Mohammad , Ebrahimi Mohammad , Aliakbari Fatemeh , Raeisi Hadi
Published: January 2023

Background
Coronavirus disease 2019 (COVID-19) has become the most important challenge in the world healthcare system today. We investigated the effect of a family-centered empowerment program on the self-efficacy and quality of life daily activity level of patients with COVID-19.
Methods
A quasi-experimental study on 60 patients with COVID-19 discharged from Shahrekord hospitals was performed. For the intervention group, family-based empowerment training was performed, which included three training sessions for patient caregivers, and for the control group, only routine discharge training was performed. all patients fulfill questionaries’ include World Health Organization (WHO) quality of life, daily activities and Sherer self-efficacy before and three months after the study. data were analyzed by SPSS/21 with chi-square, ANOVA and Pearson tests.
Results
The results of a recent study showed that family-centered empowerment program in patients leads to improved quality of life (p = 0.001) and also a significant change in the level of daily activity in the intervention group compared to the control group (p = 0.001 but there was no significant difference between the two groups in terms of self-efficacy after three months.
Conclusion
In line with the results of a recent study, studies have shown that the empowerment program for patients' family caregivers can increase patient participation and daily activity level and overall quality of life by increasing their participation in patient care. It is recommended that this program be included in day-to-day care by nurses and patient caregivers in their training programs.
Contamination characteristics in runoff fractions from a nuclear facility in São Paulo/Brazil
Authors: Faustino Mainara Generoso , Monteiro Lucilena Rebelo , Lima Samia R. M. , Silva Tatiane B. S. C. da , Pirani Debora A. , Filho Walter dos Reis Pedreira , Gonzaga Roberta G. , Cotrim Marycel E. B. , Pires Maria Aparecida F.
Published: January 2023

The stormwater runoff may act as a nonpoint pollutant source and in urban environments contributes to aquatic ecosystem quality decay. Here, we examined the runoff characteristics on the export of total solids and total metals, as well as pH and conductivity responses during the rainfall evolution. In 2017 and 2018, 12 rain events in 4 sampling stations were monitored at a parking lot located at Nuclear and Energy Research Institute (IPEN/CNEN) in São Paulo/Brazil. A 4-chamber integrated collector allowed the sequential/temporal runoff evolution assessment. The runoff was composed of Ca > K > Mg > Si > Al > Fe > Na > Zn > Mn > Sr > Ti > Mo > V > Cu > B > Pb > Ni > Ce > Sb > Cr > La > U > Th > Cd. Total solids, Al and Fe exceed Brazilian water quality standards. Principal components analysis identified the elemental clusters linked to the facility activity, soil, traffic/atmospheric related deposition. Our results show that the runoff characteristics could be differentiated by pollutant source. Seasonal variation, rain event intensity, air mass from oceanic or continental origin, spatial distribution inside the monitoring area and the intensity of the first flush must be consider to disentangle the elemental clusters and pollution source contributions. In winter, continental air masses were associated with higher concentrations heavy metals in the surface runoff. Spatial changes with no seasonal variation were observed for U, Th, La and Ce.
The impact of geriatric characteristics and comorbidities on distant metastases and other cause mortality in older women with non-metastatic breast cancer treated with primary endocrine therapy
Authors: Waaijer M.E.C. , Lemij A.A. , de Boer A.Z. , Bastiaannet E. , Bos F. van den , Derks M.G.M. , Kroep J.R. , Liefers G.J. , Portielje J.E.A. , de Glas Nienke Aafke
Published: January 2023

Introduction In recent years, primary surgical treatment of older women with non-metastatic breast cancer has decreased in favor of primary endocrine therapy (PET). PET can be considered in women with a remaining life expectancy of less than five years. The aim of this study was to 1) assess the risk of distant metastases and other cause mortality over ten years in women aged 65 and older with stage I-III breast cancer treated with PET, 2) whether this was associated with geriatric characteristics and comorbidities and to 3) describe the reasons on which the choice for PET was made.
Methods Women were included from the retrospective FOCUS cohort, which comprises all incident women diagnosed with breast cancer aged 65 or older between January 1997 and December 2004 in the Comprehensive Cancer Center Region West in the Netherlands. We selected women (N=257) with stage I-III breast cancer and treated with PET from this cohort. Patient characteristics (including comorbidity, polypharmacy, walking, cognitive and sensory impairment), treatment and tumor characteristics were retrospectively extracted from charts. Outcomes were distant metastasis and other cause mortality. Cumulative incidences were calculated using the Cumulative Incidence for Competing Risks method (CICR); and subdistribution hazard ratios (SHR) were tested between groups based on age, geriatric characteristics and comorbidity with the Fine and Gray model.
Results Women treated with PET were on average 84 years old and 41% had one or more geriatric characteristics. Other cause mortality exceeded the cumulative incidence of distant metastasis over ten years (83 versus 5.6%). The risk of dying from another cause further increased in women with geriatric characteristics (SHR 2.06, p<0.001) or two or more comorbidities (SHR 1.72, p<0.001). Surgery was omitted mainly at the patient’s request (18.7%).
Discussion This study shows that the cumulative incidence of distant metastasis is much lower than other cause mortality in older women with breast cancer treated with PET, especially in the presence of geriatric characteristics or comorbidities. This confirms the importance of assessment of geriatric characteristics to aid counseling of older women.
A Unique Glycerophospholipid Metabolism Profile of Dilated Cardiomyopathy with Predictive value
Authors: Li Xiaolei , Adi Dilare , Aizezi Aibibanmu , Wang Yongtao , Liu Fen , Li Yanhong , Li Yanpeng , Wei Xian , Kerem Munawar , Ma Xiang , Ma Yi-Tong
Published: January 2023

(1) Objects: Our aim was to identify changes in the metabolome in dilated cardiomyopathy (DCM) as well as to construct a metabolic diagnostic model for DCM.
(2) Methods: We utilized non-targeted metabolomics with a cross-sectional cohort of age- and sex-matched DCM patients and controls. Metabolomics data were analyzed using orthogonal partial least squares-discriminant analysis (OPLS-DA) and pathway analysis. It was validated in combination with transcriptome sequencing data from public databases. Machine learning models were used for the diagnosis of DCM.
(3) Results: Using multiple analytical techniques, 130 metabolite alterations were identified in DCM compared to healthy controls. Perturbations in glycerophospholipid metabolism (GPL) were identified and validated as a characteristic metabolic pathway in DCM. Through the least absolute shrinkage and selection operator (LASSO), we identified the 7 most important GPL metabolites, including LysoPA (16:0/0:0), LysoPA (18:1(9Z)/0:0), PC (20:3(8Z,11Z,14Z)/20:1(11Z)), PC (20:0/14:0), LysoPC (16:0), PS(15:0/18:0), and PE(16:0/20:4 (5Z,8Z,11Z,14Z)). The machine learning models based on the seven metabolites all had good accuracy in distinguishing DCM [All area under the curve (AUC) >0.900], and the artificial neural network (ANN) model performed the most consistently (AUC: 0.919±0.075).
(4) Conclusions: This study demonstrates that GPL metabolism may play a contributing role in the pathophysiological mechanisms of DCM. The 7-GPL metabolite model may help for early diagnosis of DCM.
A Qualitative Exploration of Pharmacists' Perspectives and Attitudes Towards the 2021 Down-scheduling of Melatonin in Australia
Authors: Yeung Kingston , Lee Samantha K. M. , Bin Yu Sun , Cheung Janet M.Y.
Published: January 2023

Background
In Australia, prescription melatonin became ‘Pharmacist Only Medicine’ for people over 55 with insomnia from June 2021. However, little is known about pharmacists’ views on melatonin down-scheduling and their perceived impacts on practice.
Objectives
To explore the impact of melatonin down-scheduling on Australian community pharmacists’ practice.
Methods
A convenience sample of community pharmacists and pharmacy interns were recruited. Participants completed a pre-interview survey capturing demographic and professional practice details, followed by a semi-structured interview. Interviews were guided by a schedule of questions exploring the perceived role of melatonin, preparation/response to down-scheduling, practice changes and patient interactions. Interviews were digitally recorded, transcribed verbatim and analysed using the Framework Approach.
Results
Twenty-four interviews were conducted with Australian community pharmacists (n = 19) and intern pharmacists (n = 5). Pharmacists/intern pharmacists welcomed the increased accessibility of melatonin for patients. However, pharmacists perceived a disconnect between the guidelines, supply protocols and pack sizes with practice, making it difficult to monitor patient use of melatonin. The miscommunication of eligibility also contributed to patient-pharmacist tension when supply was denied. Importantly, 88% of participants indicated their interest in upskilling their knowledge in melatonin use in sleep, specifically formulation differences and dosage titration.
Conclusions
This study showed the need to refine and unify supply protocols and amend pack sizes in community pharmacy. With further support and training, pharmacists have the potential to extend their scope of practice in sleep health in tandem with the provision of over-the-counter sleep aids.
Mechanical stability, electronic and magnetic properties of XZrAs (X=Cr,Mn,V) half Heusler compounds
Authors: MOKHTARI H. , BOUMIA L. , MOKHTARI M. , DAHMANE F. , MANSOUR D. , KHENATA R.
Published: January 2023

The WIEN2k code was used to perform theoretical calculations on the structural, half-metallic, and elastic properties of XZrAs (X = Cr, Mn, and V) half-Heusler compounds. For the term of the potential exchange and correlation (XC), we calculated structural, electronic, and magnetic properties using the generalized gradient approximation. In all compounds, the type 1 in the ferromagnetic (FM) phases is more energetically stable than other type arrangement. The spin-up electrons of both XZrAs (X = Cr, Mn) half-Heusler compounds in the type III structure are semiconducting with energy gaps, whereas the spin-down electrons are metallic. VZrAs compounds have also been studied, but they have metallic properties in both type I and type III structures. XZrAs (X = Cr, Mn, and V) half-Heusler compounds are elastically stable and ductile, according to calculated Cij elastic constants. Finally, XZrAs (X = Cr, Mn) half-Heusler compounds were obtained true half-metal ferromagnetic materials (HMF) within 2.99 µB/f.u. and 1.99 µB/f.u. respectively at equilibrium lattice constant a = 6.142˚A, and 6.1196 for CrZrAs and MnZrAs respectively
Correlation between COVID-19 vaccination coverage and human development index
Authors: Rezaei Fatemeh , Vali Mohebat , Aune Dagfinn
Published: January 2023

Background
COVID − 19 vaccination is essential for controlling this pandemic. Many countries have begun COVID − 19 vaccination in December 2020. This study aimed to calculate the correlation between the Human Development Index (HDI) and COVID-19 vaccination coverage.
Methods
In this ecological study for data collection a data set was prepared, which included COVID-19 vaccination coverage, HDI, and its components (Life expectancy at birth, mean years of schooling, and Gross National Income per capita), and income for 130 countries. Association between COVID-19 vaccination coverage, HDI, and its components estimated using correlation coefficients. Also, the scatter plots of the HDI and vaccination coverage were drawn according to countries income.
Results
There was a very strong positive correlation between COVID-19 vaccination coverage with HDI (r = 0.86, p < 0.001) and life expectancy at birth (r = 0.84, p < 0.001), and there was also a strong correlation with mean years of schooling (r = 0.74, p < 0.001) and Gross National Income (r = 0.73, p < 0.001). When countries were stratified by income level, these correlations were stronger for the lower middle income and upper middle income countries, than for low income and high income countries.
Conclusion
The HDI and its components was positively correlated with COVID-19 vaccination coverage. Governments should facilitate COVID-19 vaccination strategies especially in low income countries.
Association between preoperative atrial fibrillation and postoperative delirium in patients with pulmonary hypertension: A secondary analysis of a retrospective cohort study
Authors: Zhang Hong , Zhang Lei , Gan Lu , Ning Haihui , Xing Bomin , Zhang Xiaoxiao , Liu Yatao
Published: January 2023

Background Postoperative delirium is the most common neuropsychiatric disorder in major surgery. Predisposing factors have been identified, but risk factors assessment useful for managing clinical intervention, has not received unanimous consent. This work aims to investigate the relationship between atrial fibrillation and postoperative delirium in patients with pulmonary hypertension in the United States. Methods This is a secondary analysis of a retrospective cohort study. Patients with pulmonary hypertension who underwent non-cardiac, non-obstetric surgery at the University of Washington Medical Center from April 2007 to September 2013 were enrolled in the original research. The incidences of postoperative delirium were compared between patients with and without atrial fibrillation. We used multivariable logistic regression, propensity score analysis, and an inverse probability-weighting model to ensure the robustness of our findings. Results 518 patients with pulmonary hypertension (151 and 367 patients were atrial fibrillation and non-atrial fibrillation, respectively) were included in this study. The overall incidence of postoperative delirium was 3%(16/518) ; it was 1.9% (9/367) and 5.8% (7/151) for non-atrial fibrillation and atrial fibrillation patients, respectively. In the inverse probability-weighting model, atrial fibrillation was associated with high postoperative delirium (OR = 3.02, 95% CI: 1.11–8.19,P = 0.030). Conclusions Atrial fibrillation may be associated with increased risk-adjusted postoperative delirium in patients with pulmonary hypertension. Thus, these patients should be adequately assessed, monitored and controlled in early stage.
Clinical and Imaging Characteristics of Intraductal Papillary Neoplasms of the Breast: a prospective trial
Authors: Wang Yingjiao , Li Yuechong , Qu Yang , Zhou Yidong , Sun Qiang , Shen Songjie
Published: January 2023

Background
Intraductal papillary neoplasms(IPNs) often have a similar clinical and imaging presentation, making them difficult to diagnose. We designed this study to refine and compare intraductal papillary neoplasms' clinical and imaging characteristics.
Methods
We recruited patients who were diagnosed with IPN and collected their clinical, imaging, and pathological data. We compared the clinical and imaging characteristics of benign, atypical hyperplasia, and malignant lesions. We also compared the diagnostic efficacy of ultrasound and mammography.
Results
The mean age of malignant patients was 57 years old, which was significantly higher than that in the other groups (48 years in the benign group and 47 years in the atypical hyperplasia group). The proportion of patients with malignant lesions clinically presenting as palpable masses (31.3%) was significantly higher than that of benign (8.6%) (P < 0.05). The proportion of malignant lesions presenting in the periphery was 40.6% compared to 22.4% for benign (P < 0.05). In ultrasonography, characteristics that showed statistically significant differences between benign and malignant lesions were the shape of the mass and calcification (P < 0.05). In mammography, differences were found in mass shape, calcification, and density of masses and glands (P < 0.05).
Conclusions
Clinical features such as age, symptoms, lesion location, and imaging characteristics such as shape, calcification, mass, and density may help to differentiate the classifications of IPNs.
Trial registration:
This study was registered at ClinicalTrials.gov on 12/06/2020 (identifier: NCT04429269).
Improved salt-tolerance of transgenic soybean by stable over-expression of AhBADH gene from Atriplex hortensis
Authors: Yu Zhijing , Niu Lu , Cai Qinan , Wei Jia , Shang Lixia , Yang Xiangdong , Ma Rui
Published: January 2023

An effective strategy for increasing the productivity of major crops under salt stress conditions is the development of transgenics that harbor genes responsible for salinity tolerance. Betaine aldehyde dehydrogenase (BADH) is a key enzyme involved in the biosynthesis of the osmoprotectant, glycine betaine (GB), and osmotic balance in plants, and several plants transformed with BADH have shown significant improvements in salt and drought tolerance. However, very few field-tested transgenic cultivars have been reported, as most of the transgenic studies are limited to laboratory or green house experiments. In this study, we demonstrated through field experiments that BADHfrom Atriplex hortensis (AhBADH) confers salt tolerance when transformed into soybean (Glycine max L.). AhBADH was successfully introduced into soybean by Agrobacterium mediated transformation. A total of 256 transgenic plants were obtained, out of which 47 lines showed significant enhancement of salt tolerance compared to non-transgenic control plants. Molecular analyses of the transgenic line TL7 with the highest salt tolerance exhibited stable inheritance and expression of AhBADH in progenies with a single copy insertion. TL7 exhibited stable enhanced salt tolerance and improved agronomic traits when subjected to 300mM NaCl treatment. Currently, the transgenic line TL7 with stable enhanced salt tolerance, which has been cleared for environmental release, is under biosafety assessment. TL7 stably expressing AhBADH could then be applied in commercial breeding experiments in order to genetically improve salt tolerance in soybean.
Association of obesity with incident atrial fibrillation in Korea and the United Kingdom
Authors: Choi Sung Hwa , Yang Pil-Sung , Kim Daehoon , Sung Jung-Hoon , Jang Eunsun , Yu Hee Tae , Kim Tae-Hoon , Pak Hui-Nam , Lee Moon-Hyoung , Lip Gregory Y.H. , Joung Boyoung
Published: January 2023

Background: Obesity has been linked to atrial fibrillation (AF) burden and severity, and epidemiological studies suggest that AF is more prevalent in whites than Asian. We aimed to investigate whether obesity mediates associations with AF in Europe and Asia using patient-level data comparisons of two cohort studies.
Methods: Using Korean National Health Insurance Service’s Health Screening (NHIS-HealS) and U.K. Biobank data, we included 401,206 Korean and 477,926 British aged 40–70 years without previous AF who received check-ups. The incidence and risk of AF were evaluated regarding different body mass index (BMI) values.
Results: The obese proportion (BMI³30.0 kg/m2, 2.8% vs. 24.3%, P<0.001) was higher in the U.K. than the Korean. In the Korean and U.K. cohort, the age- and sex-adjusted incidence rates of AF were 4.97 and 6.54 per 1,000 person-years among obese individuals. Compared to Koreans, the risk of AF was higher in the British population, with adjusted hazard ratios of 1.41 (Korea, 95% CI, 1.26–1.58) and 1.68 (UK, 95% CI, 1.54–1.82) in obese participants (P for interaction<0.05).
Conclusions: Obesity was associated with AF in both populations. British subjects had a greater incidence of AF related to the high proportion of obese individuals, but the risk of AF was also increased when participants into obese categories.
Scale down and optimized automated production of [68Ga]68Ga-DOTA-ECL1i PET tracer targeting CCR2 expression
Authors: Migliari Silvia , Scarlattei Maura , Baldari Giorgio , Ruffini Livia
Published: January 2023

Background: recently it has been identified a short peptide that showed allosteric antagonism against C-C motif chemokine receptor 2 (CCR2) expressed on inflammatory monocyte and macrophages. A 7-D-amino acid peptidic CCR2 inhibitor called extracellular loop 1 inverso (ECL1i), d(LGTFLKC) has been identified and labeled to obtain a new probe for positron emission tomography in pulmonary fibrosis, heart injury, abdominal aortic aneurysm inflammation, atherosclerosis, head and neck cancer.
Our goal was to develop, optimize and validate an automated synthesis method and quality control system for [68Ga]68Ga-DOTA-ECL1i to make it available for a broader community.
The synthesis of [68Ga]68Ga-DOTA-ECL1i was done using the Scintomics GRP® module with the already estabilished synthesis template for [68Ga]68Ga-DOTATOC/[68Ga]68Ga-PSMA. The radiopharmaceutical production was optimized using different amount of DOTA-ECL1i (from 50 μg to 10 μg), evaluating synthesis efficiency and relevant quality control parameters in accordance with the European Pharmacopeia.
Results: best results were yielded with 20 μg DOTA-ECL1i and then the process validation was carried out by producing three different batches on three different days obtaining an optimal radiochemical yield (66,69%) as well as radiochemical purity (100%) and molar activity (45.41 GBq/µmol).
Conclusions: [68Ga]68Ga-DOTA-ECL1i was successfully synthesized and it is, thus, available for multi-dose application in clinical settings.
Structure and mechanism of the K+/H+ exchanger KefC
Authors: Gulati Ashutosh , Kokane Surabhi , Boerema Annemarie , Alleva Claudia , Meier Pascal , Matsuoka Rei , Drew David
Published: January 2023

Intracellular potassium (K+) homeostasis is fundamental to cell viability. In addition to channels, cellular K+ levels are maintained by various ion-transporters. One major family are the proton-driven K+ efflux transporters. In gram-negative bacteria, K+/H+ exchange activity is important for osmoregulation and, in plants, their activity is critical for efficient photosynthesis and growth. Despite their wide-spread importance, the structure and molecular basis for K+-selectivity is poorly understood. Here, we report the cryo-EM structure of the model glutathione (GSH)-gated K+ efflux transporter KefC from E. coli at 3.2 Å resolution. KefC forms a homodimer and is overall similar to the Na+/H+ antiporter NapA captured in an inward-facing conformation. By structural assignment of a coordinated K+ ion, MD simulations and SSM-based electrophysiology, we demonstrate how the ion-binding site in KefC is adapted for binding a dehydrated K+ ion, which contrasts with NapA, which coordinates a partially hydrated Na+ ion. The substrate-K+ ion is completely enclosed within the transport domains, consistent with an elevator alternating-access mechanism. KefC harbours C-terminal RCK regulatory domains, similar to the TrkA domain of K+-channels, forming homodimers that are stabilised by AMP and GSH. In the current KefC structure, a pair of domain-swapped helices in the RCK domains inhibit transport by directly interacting with the ion-transport domains. Taken together, we propose that KefC is activated by detachment of the RCK domains and ion-selectivity exploits the biophysical properties likewise adapted by cation ion-channels.
Bacteria from the Sonoran Desert with nematicidal potential against phytopathogenic nematodes
Authors: Chavarria-Quicaño Estefany , Contreras-Jácquez Victor , Carrillo-Fasio Armando , Torre-González Francisco De la , Asaff-Torres Ali
Published: January 2023

The use of microorganisms and/or their metabolites (e.g., Bacillus, Pseudomonas, etc.) has been proposed as an eco-friendly alternative for controlling phytopathogenic nematodes (PPNs). However, one of the challenges is that microorganisms can thrive in crops affected by climate change, salt stress and drought. Consequently, the search for microorganisms adapted to these conditions has attracted attention. Deserts are characterized by high temperatures, water scarcity, and high salinity, among other harsh conditions; hence, they are a perfect niche for isolating microorganisms adapted to extreme environments. The purpose of this work was to isolate and characterize bacteria from rhizospheric soil samples collected in the Sonoran Desert with the potential for PPN control. Approximately 100 thermotolerant bacteria were isolated, and among them the secretome of the strain identified as Bacillus paralicheniformis TB197 showed the highest nematicidal activity (96%) against Meloidogyne incognita in vitro. Additionally, in field assays, the TB197 secretome effectively suppressed infections caused by M. enterolobii. In greenhouse experiments, TB197 endospores reduced the severity of infection by 81% compared with the control (p≤0.05). Finally, the efficacy of TB197 endospores at the open field level was demonstrated against M. incognita in the tomato crop, where the galling index (GI) was reduced by 84%, and against Radopholus similisin the banana crop, where root necrosis was reduced by 81%, both compared with the controls (p≤0.05). Owing to their efficacy in controlling PNN infections, the endospores and secondary metabolites secreted by the B. paralicheniformis TB197 strain can be used to develop effective bionematicides.
Identification of target genes in recurrent implantation failure women associated with maternal-fetal immune
Authors: Fan Meiling , Liu WeiNa , Zhang Ning , Jin Xianglan , Wang Hui
Published: January 2023

Background Recurrent implantation failure (RIF) is one of the main reasons for the unsatisfactory pregnancy rate of in vitro fertilization-embryo transfer, however the underlying mechanism is still obscure. Methods An online tool (Gene Expression Omnibus 2R) was used to identify gene expression differences in RIF women and fertility controls endometrium. Publicly available databases including GeneMANIA, Predicting Associated Transcription factors from Annotated Affinities, JASPAR and Protein Analysis Through Evolutionary Relationships were accessed to perform an integrated bioinformatic analysis. RIF and fertility controls patients (n = 9, respectively) were recruited from January to June 2022 at Affiliated Hospital of Changchun University of Traditional Chinese Medicine. Levels of target genes in the downstream of predicted signal pathway were measured by qPCR. Results Twenty-one different expression genes (DEGs), C11ORF65, DAPK2, KLK3, PFKFB2, SLC17A4, SLC7A1, CDC7, CDH13, DHRS3, DZIP1, GUCY1B3, KIAA0040, KIR2DL1, MAP3K5, NFKBIZ, SESTD1, TBXAS1, TIAM1, TIMP3, TNFAIP2 and TTC39C were found co-differential expression in GSE92324 and GSE103465 series, 93.19% displayed similar co-expression characteristics. In addition, the transcription factors (TFs) associated with key DEGs were recogonized, including RSF, FOR1, C/EBPALPHA, HNF-1ALPHA, AR, DP1, E2F1 and LEF1.The TFs associated diseases were focused on chromosome breakage, female infertility, embryo loss, and thrombosis, which acted a crucial role in RIF. Biological process analysis discovered that enrichment of these TFs correlated with IL-18 signal pathway, steroid hormone mediated signal pathway, DNA transcription, reproductive process and embryonic organ development. IL-18 signal pathway associated pro-immune molecules were upregulated in endometrium of RIF women than control. Conclusion The current research identified target genes and downstream signaling pathways at the maternal-fetal interface by bioinformatics-based analyses. It may provide a clue for prognosis judgement and target therapies in RIF women.
Sea level change and human occupation over 6000 years at Areoso Island (Ria de Arousa, NW Iberia)
Authors: Cajade-Pascual Daniel , Costa-Casais Manuela , Blanco-Chao Ramón , Rodríguez Teresa Taboada
Published: January 2023

The coast is very sensitive to variations in environmental conditions. The interaction of marine and continental processes causes a high dynamism, generating depositional formations with a high value for paleoenvironmental reconstruction. This work focused on the case of two edapho-sedimentary sequences located under the current beach, near of archaeological tumuli context at Areoso Island (Ría de Arousa, NW Iberia). With a geoarchaeological approach, sea level rise, environmental conditions and human occupation over 6000 years are interpreted. The results of granulometric and mineralogical data, elemental composition and stratigraphic features, help to identify three formation environments: granite weathering, continental (soil formation and erosion) and marine-aeolian. Geomorphological evolution from the last 6.0 kyr BP has been controlled by climate and sea level change and anthropogenic causes. The continental facies evidence low sea level up to 4.8 kyr BP. After 3.2 kyr BP, the formation of a beach-dune system was verified at an elevation similar to the present one. These results are coherent with others obtained in lagoons in the Galician coast. These edapho-sedimentary sequences located in an open coastal system will help to improve the Holocene sea level rise curve in the NW Iberia and to understand the context in which the most important archaeological structures on the island, the tumuli, began to be eroded.
Methamphetamine-induced encephalopathy in the absence of hyperammonemia
Authors: Rabbany Jessica M , Fitzgerald Kaylee , Bowman Jade , Dong Fanglong , Neeki Michael M.
Published: January 2023

Background: Methamphetamine is an addictive drug with various effects on the neurotransmitters in the central nervous system. Methamphetamine-induced encephalopathy in the absence of hyperammonemia presents a unique challenge in a clinical setting. Previously published cases of methamphetamine-induced encephalopathy suggested that methamphetamine-induced hepatotoxicity and subsequent hyperammonemia may be the cause of encephalopathy. However, the literature is limited on methamphetamine-induced encephalopathy without hyperammonemia. Case: This case presents a disoriented patient following methamphetamine use disorder with acute toxicity, unable to ambulate independently, and was poorly responsive to verbal stimuli. Discussion: This patient developed encephalopathy with normal liver function tests and ammonia levels suggesting a different pathophysiological pathway for methamphetamine-induced encephalopathy. One potential pathway is through the direct action of methamphetamine on the central nervous system through acute disruption of neurotransmitter signaling and disruption of the blood-brain barrier. Conclusion: Further research should be conducted into the prevalence and pathophysiology of methamphetamine-induced encephalopathy in the absence of hyperammonemia.
Large-scare subsurface flow constructed wetland for marine aquaculture water purification: functional microbial detection
Authors: Chen Chen , Li Pengquan , Zhao Weihua , Chen Jingfei , Yan Maocang , Guo Chong , Fu Min , Xu Zhenxing , Chen Xuechu
Published: January 2023

In order to improve the quality of natural seawater to meet the needs of aquaculture production, a large-scale subsurface flow constructed wetland was constructed and operated stably for 2 years to study its purification effect on natural seawater. Studies had shown that the system could maintain high purification effect on natural seawater during aquaculture, and could effectively remove harmful algae such as Cyanobacteria, especially in warm months. The pyrosequencing of 16S ribosomal DNA revealed that Proteobacteria, Nitrospirae, chloroflexi were the main and key bacterial phyla in the system. Members of these key gates were regarded as the important roles in resisting water purification. In summary, the subsurface flow wetland system was suitable for large-scale seawater aquaculture plants, which could effectively improve seawater quality conditions and reduced the density of harmful algae cells.
CircACLY regulates proliferation, differentiation and apoptosis of Jingyuan chicken myoblasts by sponging gga-miR-6660-3P
Authors: Zhang Juan , Wang Weizhen , Cai Zhengyun , Yu Baojun , Zhao Wei , Fu Xi , Wang Tailu , Gu Yaling , Xin Guosheng
Published: January 2023

Circular RNAs (circRNAs) are key gene regulators that are involved in many fundamental biological processes. Skeletal muscle cannot develop normally without the involvement of circRNA. We found that a circRNA from the ATP Citrate Lyase (ACLY) gene regulates skeletal muscle development through sponging miRNA. The results of ribonuclease R (RNase R) digestion and subcellular localization assays verified that circACLY is a circRNA that is mainly localized to the cytoplasm. Dual-luciferase and co-transfection assay findings showed that gga-miR-6660-3p binds directly to the predicted binding sites of circACLY and Ectonucleotide Triphosphate Diphosphohydrolase 7 (ENTPD7). Furthermore, circACLY increased the expression of ENTPD7 by adsorbing gga-miR-6660-3p. Cell proliferation assays showed that circACLY promoted, whereas gga-miR-6660-3p inhibited myoblast proliferation. Assays of induced differentiation, differentiation marker gene mRNA expression and protein levels showed that gga-miR-6660-3p inhibited, whereas circACLY facilitated myoblast differentiation. Furthermore, circACLY inhibited, whereas gga-miR-6660-3P promoted myoblast apoptosis, and they regulate inosine monophosphate synthesis (IMDNS) de novo by respectively promoting and inhibiting it. Our findings confirmed that circACLY from the ACLY gene regulates IMDNS by sponging- gga-miR-6660-3P to promote myoblast proliferation and differentiation and inhibit myoblast apoptosis.
Antibacterial activity of Clinoptilolite/TiO 2 /ZnO photocatalyst against Escherichia coli and Staphylococcus aureus in water resources
Authors: Abdi Maryam , Anbia Mansoor , safavi Maliheh , Sepehrian Mohammad , Nezafatian Elham
Published: January 2023

Water treatment is crucial due to rising water demand across the board, including drinking, agriculture, industry, etc. Pathogens are a type of contamination that must be studied to improve water purification methods. This study investigates the elimination of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) using Clinoptilolite/TiO2/ZnO nanocomposite with varied TiO2/ZnO ratios of 1:1, 1:2, and 2:1 under UVA and UVC light irradiation. To synergize the effect of coupling TiO2 with ZnO, we synthesized nanocomposite Clinoptilolite/TiO2/ZnO to make a powerful system for water and wastewater disinfection. The characterization tests, including X-ray diffraction, field emission scanning electron microscopy, X-ray energy diffraction, Fourier-transform infrared spectroscopy, diffuse reflection spectroscopy, and nitrogen adsorption-desorption, were performed to confirm the structure. The optimum response for bacterial removal was found by using the determination-optimal (D-optimal) design of response surface methodology (RSM). 100% removal was obtained for E. coli and S. aureus under UVA irradiation and contact time of 100 min for both of them.
The effect of twice and four activations per day of periodontal distractor on pain intensity and pulp vitality of maxillary canine
Authors: driny Alaa M. El , Salama Ahmed A. , El-Shal Osama S. , Mohamed Maha M.
Published: January 2023

Objectives
No study has compared pain intensity and pulp vitality of both activation protocols of periodontal distractor (twice and four activations per day) for rapid canine retraction. Since the periodontal distractor may cause some degree of suffering for the patients and may affect pulp vitality, it is important for orthodontists to handle this situation in the best possible way by choosing the most effective and the least painful method of treatment. Therefore the aim of the present study was to evaluate the effect of twice and four activations/day of periodontal distractor on pain intensity and pulp vitality of maxillary canine.
Methods
The study was carried out on 32 canine of 16 female patients (16–22 years of age) requiring extraction of bilateral maxillary first premolars and maximum anchorage. They were divided into two groups. (Group I): Canine retraction was done by periodontal distractor with twice activations per day. (Group II): Canine retraction was done by periodontal distractor with four activations per day. Periodontal distractor was cemented after bilateral maxillary premolar extraction and inter septal bone corticotomy was done. Pain intensity was evaluated at different times by Visual Analogue scale (1st 48 hour after intervention, during each activation of the distractor, and on the Interval between activation).Pulp vitality was measured by electric pulp tester.
Results
Comparison of pain intensity between different intervals was performed by using Friedman`s test (non-parametric data) which revealed significant difference in both groups, as 1st 48 hours was significantly the highest (8.8 ± 0.77), (9.2 ± 0.77) followed by During each activation (5.4 ± 0.94), (6.9 ± 1.25), while Interval between activation (1 ± 1.03), (2.7 ± 1.38) regarding group I and II respectively. Comparison between both groups was performed by using Mann Whitney`s test (non-parametric), which revealed that there was significant difference during each activation and intervals between activations as group II was significantly higher than group I (P = 0.0001).None of the teeth reacted negatively to the electrical vitality test that was performed one month after the completion of the distraction procedure. There was no clinical sign of discoloration or pulpal pain in any tooth.
Conclusion
In this study, Pain intensity was y significantly higher in group II (four activations/ day) than group I (twice activations/ day). The pulp of the retracted canine teeth examined in this study for both groups was vital on both groups.
SOX2 predicts local spreading and promotes a cancer stem cell-like phenotype in oral squamous cell carcinoma
Authors: Sacco Alessandro , Battaglia Anna Martina , Santamaria Gianluca , Buffone Caterina , Barone Selene , Procopio Anna , Lavecchia Anna Maria , Aversa Ilenia , Cristofaro Maria Giulia , Giudice Amerigo , Biamonte Flavia
Published: January 2023

Background
Emerging evidence points toward the idea that oral squamous cell carcinoma (OSCC) invasiveness can be attributed to the existence of a small subpopulation of cancer stem cells (CSCs) in the bulk of the tumor. However, knowledge about the existence of CSCs in the OSCC close margins and their possible role in determining OSCC phenotypes is still far from being satisfactory.
Methods
We analyzed the expression of 13 CSCs marker genes in 72 primary tissue specimens (24 core tumors, 24 pathologically negative close margins, and 24 healthy distant margins), obtained from 24 OSCC patients upon surgical resection, by using Real-Time PCR. TGCA and GEO databases were used to confirm gene expression analyses. CAL27 and SCC15 OSCC cell lines were used to perform in vitro siRNA-mediated knockdown of SOX2 both in 2D and 3D cultures. The effects of SOX2 knockdown on OSCC cell lines were assessed by wound healing assays and 3D tumor spheroids formation assays. Cytofluorimetric apoptosis assays were used to test the effects of SOX2 knockdown on cisplatin sensitivity. Live imaging was used to monitor OSCC cells migration and spheroidogenesis.
Results
We found that BMI1, CD44, SOX2, OCT4, UBE2C, CXCR4 were significantly up-regulated, while IGF1-R, KLF4, ALDH1A1, CD133, FAM3Cappeared down-regulated in the tumor core vs healthy distant margin. No differences were observed for NANOG and RRM2. SOX2, CD44, and CXCR4 were upregulated also in the close margin vs healthy distant margin. The high expression of SOX2 both in the tumor core and in the close margin significantly correlated with tumor size and lymph node compromise. In vitro experiments further showed that SOX2knockdown i) promotes the mesenchymal-to-epithelial transition and smooths the invasiveness, ii) attenuates the 3D tumor sphere-forming capacity and stemness-associated traits and iii) partially increases the sensitivity to cisplatin treatment of CAL27 and SCC15 cell lines.
Conclusions
Overall, our study shows that the OSCC close margins can retain CSC-specific markers even in the setting of negative histopathological diagnosis. In particular, SOX2 may represent a promising marker useful to predict a more aggressive OSCC phenotype and a suitable target to prevent local invasiveness.
Morphological abnormalities of orbitoidal foraminifera (Orbitoides and Omphalocyclus) from the Maastrichtian of the Zagros Mountains in northern Iraq
Authors: Nuaimy Qahtan A.M. Al
Published: January 2023

Many Orbitoides and Omphalocyclus individuals that were collected in the areas of Sura Qalat and Mokaba (northeast Sulaimani, Iraq) exhibited a number of aberrant characteristics. They contain exceptional examples of the microboring activity that took place within the tests, an uncommon test exterior form, the blossoming of equatorial chamber development, and peculiar reproductive characteristics. These aspects provide evidence for the existence of orbitoidal foraminiferal fossilized remains throughout the Maastrichtian period, as well as for palaeoecological variables, the biological lifetime, and taxonomic classification. The fossilization of these individuals and their associations with other creatures, such as Maeandropolydora osmaneliensis Nielsen, Gormüş, and Curvichnus semorbis Nielsen, are what induce the activity known as microboring. Conical forms and other unusual exterior shapes are thought to be linked to palaeoenvironmental sediment logical features such as the type of substratum and the currents that existed in that environment. It is believed that the biological and genetic traits of orbitoidal taxa have a considerable impact on the formation of lateral and equatorial chambers, in addition to the distinctive reproductive abilities these chambers possess. In the case of Orbitoides apiculatus Schlumberger, one of the most notable features is thought to be the cyclical flowering that occurs in the equatorial chambers.
Assessment of diclofenac in three selected rivers of Western Himalayan Region
Authors: Sharma Dheeraj , Choudhary Poonam , Gandotra Roopma
Published: January 2023

The present study reports the concentration of Non-steroidal anti- inflammatory drug (NSAID), Diclofenac in three main rivers namely Sarsa, Sutluj and Ravi of Western-Himalayan region. Diclofenac was analyzed from water samples using UPLC-S/MS system. The concentration ranged between 0- 2.05ng/l, the maximum concentration was observed in Badhi (S1) due to presence of number of pharmaceutical industries. The concentrations of diclofenac reported in the current study were comparatively very low as observed in other studies worldwide. This is the first report on the occurrence of diclofenac in the rivers of Western Himalayan region.
Spontaneous Bilateral Internal Carotid Arteries Dissection with Acute Stroke - A Case for Stent Implantation and Antithrombotic Therapy and literature review
Authors: li hong-yan , Wang Long-bing , Cai Zhi-you , Li Jing-lun
Published: January 2023

Background
Internal Carotid Arteries Dissection (ICAD) is a rare but important cause of severe acute ischemic stroke. Patients with ICAD are mostly treated with the antithrombotic regimen (anticoagulation, signal antiplatelet and dual antiplatelet), or endovascular treatment. The recurrence rate of carotid artery dissection stroke is relatively low. Here, we present a case of middle-aged male patient with acute ischemic strokes was caused by spontaneous bilateral ICAD. Stent placement in combine with antiplatelet therapy is a beneficial treatment method for such patient, as a preferred option.
Case presentation:
We present a case of a 54 -year-old male with no history of arteriopathy who presented with left limber weakness and slurred speech. Cerebral magnetic resonance imaging (MRI) showed acute large area cerebral infarcts in the right hemisphere. Digital subtraction angiography (DSA) demonstrated that the right internal carotid artery (ICA) occlusion at the cervical portion. He was found to have dissections in the right intracranial carotid arteries. He was treated with dual antiplatelet therapy and right carotid artery stent implantation. However, the patient still developed new neurological deficits, after stenting and balloon dilatation in the right carotid artery and postoperative antiplatelet therapy. MRI showed that acute left cerebral hemisphere infarction. Cervical computed tomography angiography and DSA revealed status of right carotid artery stent implantation and left ICA occlusion. Similarly, stent was performed in his left carotid artery, and dual antiplatelet therapy still continued. He was seen in the clinic at 6 months with follow-up imaging revealing improvement in the dissections.
Conclusion
The preferred option for patients with ICAD is to receive medication. If the ischemic event still occurs, invasive treatment should be carried out. However, ischemic stroke events were treated effectively with stenting and postoperative antiplatelet therapy.