bluuebunny/crossref_metadata_2025 huggingface dataset:
A Gene-Based Method for Cytogenetic Mapping of Repeat-Rich Mosquito Genomes
Authors: Masri Reem A. , Karagodin Dmitriy A. , Sharma Atashi , Sharakhova Maria V.
Published: February 2021

Long-read sequencing technologies have opened up new avenues of research on the mosquito genome biology, enabling scientists to better understand the remarkable abilities of vectors for transmitting pathogens. Although new genome mapping technologies such as Hi-C scaffolding and optical mapping may significantly improve the quality of genomes, only cytogenetic mapping, with the help of fluorescence in situ hybridization (FISH), connects genomic scaffolds to a particular chromosome and chromosome band. This mapping approach is important for creating and validating chromosome-scale genome assemblies for mosquitoes with repeat-rich genomes, which can potentially be misassembled. In this study, we describe a new gene-based physical mapping approach that was optimized using the newly assembled Aedes albopictus genome, which is enriched with transposable elements. To avoid amplification of the repetitive DNA, 15 protein-coding gene transcripts were used for the probe design. Instead of using genomic DNA, complementary DNA was utilized as a template for development of the PCR-amplified probes for FISH. All probes were successfully amplified and mapped to specific chromosome bands. The genome-unique probes allowed to perform unambiguous mapping of genomic scaffolds to chromosome regions. The method described in detail here can be used for physical genome mapping in other insects.
COVID-19 in Autoinflammatory Diseases with Immunosuppressive Treatment
Authors: Welzel Tatjana , Samba Samuel Dembi , Klein Reinhild , van den Anker Johannes N. , Kuemmerle-Deschner Jasmin B.
Published: February 2021

COVID-19 disease increases interleukin (IL)-1β release. Anti-IL-1-treatment is effective in IL-1-mediated autoinflammatory diseases (AID). This case series presents COVID-19 in patients with IL-1-mediated and unclassified AID with immunosuppressive therapy (IT). Patient 1 is a 34-year-old woman with an unclassified AID and methotrexate. Patients 2 and 3 (14-year-old girl and 12-year-old boy, respectively) have a Cryopyrin-Associated Periodic Syndrome (NLRP3 p.Q703K heterozygous, CAPS) treated with canakinumab 150 mg/month since three and five years, respectively. Patient 4 is a 15-year-old girl who has had familial Mediterranean fever (MEFV p.M694V homozygous) for 3 years treated with canakinumab 150 mg/month and colchicine. All patients had a mild acute COVID-19 course, particularly the adolescent patients. A few weeks after COVID-19 recovery, both CAPS patients developed increased AID activity, necessitating anti-IL-1-treatment intensification in one patient. At day 100, one out of four patients (25%) showed positive antibody response to SARS-CoV-2. This is one of the first reports providing follow-up data about COVID-19 in AID. The risk for severe acute COVID-19 disease was mild/moderate, but increased AID activity post-COVID-19 was detected. Follow-up data and data combination are needed to expand understanding of COVID-19 and SARS-CoV-2 immunity in AID and the role of IT.
Advances in Membranous Nephropathy
Authors: Ronco Pierre , Plaisier Emmanuelle , Debiec Hanna
Published: February 2021

Membranous nephropathy (MN) is a rare auto-immune disease where the glomerulus is targeted by circulating auto-antibodies mostly against podocyte antigens, which results in the formation of electron-dense immune complexes, activation of complement and massive proteinuria. MN is the most common cause of nephrotic syndrome in adults leading to severe thrombotic complications and kidney failure. This review is focused on the recent therapeutic and pathophysiological advances that occurred in the last two years. For a long time, we were lacking a head-to-head comparison between cyclophosphamide considered as the gold standard therapy and other medications, notably rituximab. Substantial progress has been achieved owing to three randomized controlled trials. MENTOR (Membranous Nephropathy Trial of Rituximab) and STARMEN (Sequential Therapy with Tacrolimus and Rituximab in Primary Membranous Nephropathy) conclusively established that calcineurin inhibitor-based regimens are slower to result in an immunologic response than rituximab or cyclophosphamide, achieve fewer complete clinical remissions, and are less likely to maintainremission. Rituximab Versus Steroids and Cyclophosphamide in the Treatment of Idiopathic Membranous Nephropathy (RI-CYCLO) suggested that competition between cyclophosphamide and rituximab remains open. Given the technological leap combining laser microdissection of glomeruli and mass spectrometry of solubilized digested proteins, four “new antigens” were discovered including NELL-1 and Semaphorin 3B in so-called primary MN, and exostosins 1 and 2 and NCAM 1 in lupus MN. NELL-1 is associated with about 8% of primary MN and is characterized by segmental immune deposits and frequent association with cancer (30%). Semaphorin 3B-associated MN usually occurs in children, often below the age of two years, where it is the main antigen, representing about 16% of non-lupus MN in childhood. Exostosins 1/2 and NCAM 1 are associated with 30% and 6% of lupus MN, respectively. Exostosins 1/2 (EXT1/2) staining is associated with a low rate of end-stage kidney disease (ESKD) even in mixed classes III/IV+V. These findings already lead to revisiting the diagnostic and therapeutic algorithms toward more personalized medicine.
Women after Bilateral Surgical Correction of Hallux Valgus Do not Show Improvement in Spatiotemporal Gait Parameters at 18 Weeks Postoperatively
Authors: Kaczmarczyk Katarzyna , Barton Gabor J. , Wiszomirska Ida , Wychowanski Michal
Published: February 2021

Background: Hallux valgus (HV) is a gait-altering orthopedic deformity, somewhat more prevalent in women, which often affects both limbs. Although surgery is a commonly applied treatment, there is no consensus in the literature on how invasive HV correction affects spatiotemporal gait parameters, or how quickly improvement can be expected. We investigated gait parameters in female HV patients who underwent bilateral surgical correction of hallux valgus, both preoperatively and 18 weeks following surgery (a timeframe relevant from the perspective of physical therapy), and also in relation to a non-HV control group. Methods: A total of 23 women aged 40–70 years, with moderate to severe HV deformity in both feet, were assessed preoperatively and 18 weeks postoperatively, and an age-matched control group of 76 healthy women was also assessed. A total of 22 spatiotemporal parameters were collected during 30 s walks over an electronic walkway (Zebris Medical System). Results: Of the 22 parameters analyzed, significant differences between the preoperative experimental and control groups were found only in 4 parameters (Velocity, Right step time, Total double support and Stride time), but in 16 parameters between the postoperative experimental and control groups (the greatest impact being found for: Left and Right Step time, Stride time, Cadence, Right Foot rotation, Left Step length (% leg length) and Stride length (% leg length)). Conclusions: Women after bilateral HV correction did not exhibit improved (i.e., more normal) gait parameters at 18 weeks postoperatively; rather, they showed more gait abnormalities than preoperatively. These findings urge longer-term planning of postoperative rehabilitation, involving continual evaluation of gait improvement.
Optical Coherence Tomography Angiography of Peripapillary Vessel Density in Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorder: A Comparative Study
Authors: Rogaczewska Małgorzata , Michalak Sławomir , Stopa Marcin
Published: February 2021

Multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD) are demyelinating diseases of the central nervous system, which differ in the pathogenic mechanism. A common clinical presentation of both conditions is optic neuritis (ON). The study aimed to compare the radial peripapillary capillary (RPC) vessel density in MS and NMOSD patients using optical coherence tomography angiography (OCTA). A total of 40 MS patients, 13 NMOSD patients, and 20 controls were included. The average RPC vessel density was significantly lower in ON eyes (MS+ON, NMOSD+ON) than in non-ON eyes (MS−ON, NMOSD−ON) and in MS+ON, MS−ON, NMOSD+ON, and NMOSD−ON compared with the control group. In NMOSD+ON eyes, the vessel density in superior nasal, nasal superior, and inferior sectors was significantly more decreased than in MS+ON eyes. RPC reduction was also observed in inferior nasal and temporal superior sectors in MS−ON eyes compared with NMOSD−ON eyes. In conclusion, our findings indicate that optic neuritis is associated with a more significant RPC vessel density drop in NMOSD than in MS patients, and the predilection to superior and inferior sectors may be useful as a differential diagnostic marker.
Soluble Pecam-1 as a Biomarker in Periprosthetic Joint Infection
Authors: Fuchs Michael , Trampuz Andrej , Kirschbaum Stephanie , Winkler Tobias , Sass F Andrea
Published: February 2021

A reliable workup with regard to a single diagnostic marker indicating periprosthetic joint infection (PJI) with sufficient sensitivity and specificity is still missing. The immunologically reactive molecule Pecam-1 is shed from the T-cell surface upon activation via proinflammatory signaling, e.g., triggered by specific pathogens. We hypothesized that soluble Pecam-1 (sPecam-1) can hence function as a biomarker of PJI. Fifty-eight patients were prospectively enrolled and assigned to one of the respective treatment groups (native knees prior to surgery, aseptic, and septic total knee arthroplasty (TKA) revision surgeries). Via synovial sample acquisition and ELISA testing, a database on local sPecam-1 levels was established. We observed a significantly larger quantity of sPecam-1 in septic (n = 22) compared to aseptic TKA revision surgeries (n = 20, p ≤ 0.001). Furthermore, a significantly larger amount of sPecam-1 was found in septic and aseptic revisions compared to native joints (n = 16, p ≤ 0.001). Benchmarking it to the gold standard showed a high predictive power for the detection of PJI. Local sPecam-1 levels correlated to the infection status of the implant, and thus bear a strong potential to act as a biomarker of PJI. While a clear role of sPecam-1 in infection could be demonstrated, the underlying mechanism of the molecule’s natural function needs to be further unraveled.
Urinary Titin N-Fragment as a Biomarker of Muscle Atrophy, Intensive Care Unit-Acquired Weakness, and Possible Application for Post-Intensive Care Syndrome
Authors: Nakanishi Nobuto , Tsutsumi Rie , Hara Kanako , Matsuo Masafumi , Sakaue Hiroshi , Oto Jun
Published: February 2021

Titin is a giant protein that functions as a molecular spring in sarcomeres. Titin interconnects the contraction of actin-containing thin filaments and myosin-containing thick filaments. Titin breaks down to form urinary titin N-fragments, which are measurable in urine. Urinary titin N-fragment was originally reported to be a useful biomarker in the diagnosis of muscle dystrophy. Recently, the urinary titin N-fragment has been increasingly gaining attention as a novel biomarker of muscle atrophy and intensive care unit-acquired weakness in critically ill patients, in whom titin loss is a possible pathophysiology. Furthermore, several studies have reported that the urinary titin N-fragment also reflected muscle atrophy and weakness in patients with chronic illnesses. It may be used to predict the risk of post-intensive care syndrome or to monitor patients’ condition after hospital discharge for better nutritional and rehabilitation management. We provide several tips on the use of this promising biomarker in post-intensive care syndrome.
Assessing Problematic Social Media Use in Adolescents by Parental Ratings: Development and Validation of the Social Media Disorder Scale for Parents (SMDS-P)
Authors: Austermann Maria Isabella , Thomasius Rainer , Paschke Kerstin
Published: February 2021

Background: The problematic use of social media (SM) is a rising phenomenon, especially in adolescents. It can be assessed by self-rating screeners such as the Social Media Disorder Scale (SMDS). However, young age or symptom denial might reduce adolescent assessment accuracy. Therefore, the development and validation of a parental scale (SMDS-P) is desirable. Method: A representative sample of 961 parents and corresponding frequently SM-using children aged 10 to 17 years participated in an online study. Factorial analyses were performed to determine item structure. Adolescents’ SMDS self-reports, SM usage time, emotional dysregulation, and academic performance were used to assess validity. The SMDS-P cut-off value was calculated by ROC-analysis. Results: A one-factorial structure of the SMDS-P could be confirmed. The internal consistency was good (Cronbach’s α = 0.85, McDonald’s ω = 0.88) and the accordance between parental and self-ratings moderate (kappa = 0.51). SMDS-P was positively associated with adolescents’ self-ratings (r = 0.68), SM usage time (r = 0.26) and frequency (ϱ = 0.16) as well as with emotional dysregulation (r = 0.35) in a highly significant manner. Conclusions: SMDS-P offers a promising new approach to assess problematic SM usage in adolescence. Further studies including clinical validations are required.
The Growing Role of Echocardiography in Pulmonary Arterial Hypertension Risk Stratification: The Missing Piece
Authors: Miotti Cristiano , Papa Silvia , Manzi Giovanna , Scoccia Gianmarco , Luongo Federico , Toto Federica , Malerba Claudia , Cedrone Nadia , Sciomer Susanna , Ciciarello Francesco , Fedele Francesco , Vizza Carmine Dario , Badagliacca Roberto
Published: February 2021

Pulmonary arterial hypertension (PAH) is a rare, progressive disease with a poor prognosis. The pathophysiologic model is mainly characterized by an afterload mismatch in which an increased right ventricle afterload, driven by increased pulmonary vascular resistance (PVR), leads to right heart failure. International guidelines recommend optimization of treatment based on regular risk assessments to achieve or maintain a low-risk status. Current risk scores are based on a multi-modality approach, including demographic, clinical, functional, exercise, laboratory, and hemodynamic parameters, which lack significant echocardiographic parameters. The originality of echocardiography relies on the opportunity to assess in a non-invasive way a physiologically meaningful combination of easy to measure variables tightly related to right ventricle adaptation/maladaptation to increased afterload, the main determinant of a patient’s prognosis. Echo-derived morphological and functional parameters have been investigated in PAH, proving to have prognostic relevance. Different therapeutic strategies proved to have different effects in reducing PVR. An upfront combination of drugs, including a parenteral prostacyclin, has shown to be associated with right heart reverse remodeling in a greater proportion of patients than other treatment strategies as a function of PVR reduction. Adding echocardiographic data to current risk scores would allow better identification of right ventricle (RV) adaptation in PAH patients’ follow-up. This additional information would allow better stratification of the patient, leading to optimized and personalized therapeutic management.
Impact of the COVID-19 Pandemic on Maternal Anxiety in Brazil
Authors: Nomura Roseli , Tavares Isabela , Ubinha Ana , Costa Maria , Opperman Maria , Brock Marianna , Trapani Alberto , Damasio Lia , Reis Nadia , Borges Vera , Zaconeta Alberto , Araujo Ana , Ruano Rodrigo ,   BrAPS-COVID Brazilian Anxiety during Pregnancy Study Group in COVID-19
Published: February 2021

Background: The coronavirus disease 2019 (COVID-19) pandemic may have an effect on maternal anxiety and influence postpartum experience. Objective: To study the prevalence of maternal anxiety in late pregnancy in the context of the COVID-19 outbreak in Brazil and to analyze its association with maternal knowledge and concerns about the pandemic. Methods: This was a national multicenter cross-sectional study performed in 10 different public university hospitals, between 1 June and 31 August 2020, in Brazil. The inclusion criteria were: maternal age more than 18 years; gestational age more than 36 weeks at childbirth; single alive newborn without malformations; and absence of mental disorders. We applied a structured questionnaire to explore the knowledge and concerns about COVID-19. Maternal anxiety was assessed using the Beck Anxiety Inventory (BAI). Results: Of the 1662 women interviewed, the BAI score in late pregnancy indicated that 13.9% presented moderate and 9.6% severe maternal anxiety. Moderate or severe maternal anxiety was independently associated with the fear of being unaccompanied at childbirth (aOR1.12, 95% CI 1.10–1.35), and independent protective factors were confidence in knowing how to protect oneself from COVID-19 (aOR0.89, 95% CI 0.82–0.97) and how to safely breastfeed (aOR0.89, 95% CI 0.83–0.95). Conclusion: The COVID-19 pandemic has a significant impact on maternal anxiety.
Metabolomics as a Promising Resource Identifying Potential Biomarkers for Inflammatory Bowel Disease
Authors: Bauset Cristina , Gisbert-Ferrándiz Laura , Cosín-Roger Jesús
Published: February 2021

Inflammatory bowel disease (IBD) is a relapsing chronic disorder of the gastrointestinal tract characterized by disruption of epithelial barrier function and excessive immune response to gut microbiota. The lack of biomarkers providing early diagnosis or defining the status of the pathology difficulties an accurate assessment of the disease. Given the different metabolomic profiles observed in IBD patients, metabolomics may reveal prime candidates to be studied, which may help in understanding the pathology and identifying novel therapeutic targets. In this review, we summarize the most current advances describing the promising metabolites such as lipids or amino acids found through untargeted metabolomics from serum, faecal, urine and biopsy samples.
Inspection of Transparent Objects with Varying Light Scattering Using a Frangi Filter
Authors: Gruber Dieter P. , Haselmann Matthias
Published: February 2021

This paper proposes a new machine vision method to test the quality of a semi-transparent automotive illuminant component. Difference images of Frangi filtered surface images are used to enhance defect-like image structures. In order to distinguish allowed structures from defective structures, morphological features are extracted and used for a nearest-neighbor-based anomaly score. In this way, it could be demonstrated that a segmentation of occurring defects is possible on transparent illuminant parts. The method turned out to be fast and accurate and is therefore also suited for in-production testing.
Investigating the Structure of Intelligence Using Latent Variable and Psychometric Network Modeling: A Commentary and Reanalysis
Authors: Schmank Christopher J. , Goring Sara Anne , Kovacs Kristof , Conway Andrew R. A.
Published: February 2021

In a recent publication in the Journal of Intelligence, Dennis McFarland mischaracterized previous research using latent variable and psychometric network modeling to investigate the structure of intelligence. Misconceptions presented by McFarland are identified and discussed. We reiterate and clarify the goal of our previous research on network models, which is to improve compatibility between psychological theories and statistical models of intelligence. WAIS-IV data provided by McFarland were reanalyzed using latent variable and psychometric network modeling. The results are consistent with our previous study and show that a latent variable model and a network model both provide an adequate fit to the WAIS-IV. We therefore argue that model preference should be determined by theory compatibility. Theories of intelligence that posit a general mental ability (general intelligence) are compatible with latent variable models. More recent approaches, such as mutualism and process overlap theory, reject the notion of general mental ability and are therefore more compatible with network models, which depict the structure of intelligence as an interconnected network of cognitive processes sampled by a battery of tests. We emphasize the importance of compatibility between theories and models in scientific research on intelligence.
Minimization of the Line Resistance Impact on Memdiode-Based Simulations of Multilayer Perceptron Arrays Applied to Pattern Recognition
Authors: Aguirre Fernando Leonel , Gomez Nicolás M. , Pazos Sebastián Matías , Palumbo Félix , Suñé Jordi , Miranda Enrique
Published: February 2021

In this paper, we extend the application of the Quasi-Static Memdiode model to the realistic SPICE simulation of memristor-based single (SLPs) and multilayer perceptrons (MLPs) intended for large dataset pattern recognition. By considering ex-situ training and the classification of the hand-written characters of the MNIST database, we evaluate the degradation of the inference accuracy due to the interconnection resistances for MLPs involving up to three hidden neural layers. Two approaches to reduce the impact of the line resistance are considered and implemented in our simulations, they are the inclusion of an iterative calibration algorithm and the partitioning of the synaptic layers into smaller blocks. The obtained results indicate that MLPs are more sensitive to the line resistance effect than SLPs and that partitioning is the most effective way to minimize the impact of high line resistance values.
DNA Sequence Analyses Reveal Two New Species of Caloglossa (Delesseriaceae, Rhodophyta) from the Skin of West Indian Manatees
Authors: Freshwater D. Wilson , Miller Cathryn E. , Frankovich Thomas A. , Wynne Michael J.
Published: February 2021

Epizoic macroalgae collected from the skin of West Indian manatees included specimens of the red algal family Delesseriaceae. Morphological and rbcL sequence analyses indicated that these specimens represented two novel species of Caloglossa. One species, described here as Caloglossa kamiyana Freshwater, Cath.E. Miller & Frankovich sp. nov., had been previously studied and recognized as part of the C. ogasawaraensis species complex. The rbcL sequence divergence between C. kamiyana and other taxa within the complex ranged from 4.6–5.3%, and tetrasporangial mother cells are cut off from the lateral pericentral cells by oblique divisions instead of transverse divisions as in C. ogasawaraensis. The second species was resolved as a closely related sister species to C. fluviatilis, with a minimum interspecific sequence divergence of 2.0%. It was morphologically indistinguishable from C. fluviatilis except for one potential character—mostly one, instead of multiple rhizoids, developing from rhizoid-bearing pericentral and marginal wing cells. It is herein described as Caloglossa manaticola Freshwater, Cath.E. Miller & Frankovich sp. nov.
Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus
Authors: Masilela Charity , Pearce Brendon , Ongole Joven Jebio , Adeniyi Oladele Vincent , Benjeddou Mongi
Published: February 2021

Aims: To examine the association of polymorphisms belonging to SLC22A1, SP1, PRPF31, NBEA, SCNN1B, CPA6 and CAPN10 genes with glycaemic response to metformin and sulphonylureas (SU) combination therapy among South African adults with diabetes mellitus type 2 (T2DM). Methods: A total of 128 individuals of Swati (n = 22) and Zulu (n = 106) origin attending chronic care for T2DM were recruited. Nine SNPs previously associated with metformin and SUs were selected and genotyped using MassArray. Uncontrolled T2DM was defined as HbA1c > 7%. The association between genotypes, alleles and glycaemic response to treatment was determined using multivariate logistic regression model analysis. Results: About 85.93% (n = 110) of the study participants were female and 77.34% (n = 99) had uncontrolled T2DM (HbA1c > 7%). In the multivariate (adjusted) logistic regression model analysis, the CC genotype of rs2162145 (CPA6), GG and GA genotypes of rs889299 (SCNN1B) were significantly associated with uncontrolled T2DM. On the other hand, the C allele of rs254271 (PRPF31) and the GA genotype of rs3792269 (CAPN10) were associated with controlled T2DM. A significant interaction between rs2162145 and rs889299 in response to metformin and SU combination therapy was observed. Conclusions: In this study, we reported the association of rs2162145 (CC) and rs889299 (GG and GA) with uncontrolled T2DM. We also reported the association of rs254271 (C) and rs3792269 (GA) with controlled T2DM in response to metformin and SU combination therapy. Furthermore, an interaction between rs2162145 and rs889299 was established, where the genotype combination GA (rs889299) and TT (rs2162145) was associated with uncontrolled T2DM.
Fine Art Insurance Policies and Risk Perceptions: The Case of Malta
Authors: Pavia Luke , Grima Simon , Romanova Inna , Spiteri Jonathan V.
Published: February 2021

The aim of this paper is to identify the risks that need to be addressed when holding fine art, determine which are perceived as being the most important, and whether the risk perception is influenced by demographic variables such as age, educational background, and field of occupation. To identify the risks and evaluate the risk perception, we used a purposely designed questionnaire and sent it via various sources of communication systems and applications to individuals knowledgeable on fine arts. Findings revealed that, generally, art deterioration, art fraud, and art theft are the three main highlighted risks, with art deterioration considered in the high-risk range. In terms of risk perception, forgery is the biggest concern. On the other hand, considerations of the investment value of art lessened perceived risk exposure. Furthermore, the study has shown that certain risk perceptions were influenced by the participants’ demographic variables. Both the identified risks and risk perception considerations analyzed within this study provide us with insights as to what needs to be considered when offering fine art insurance, particularly when it comes to which risks that are perceived as being the most pressing by potential policyholders, and how these perceptions vary according to individual demographics variables as noted above.
Modeling Study on Risk Identification in the Process of Anti-Crisis Enterprise Management
Authors: Shamborovskyi Grygoriy , Nehoda Yuliia , Demidova Nataliya , Tarashchenko Volodymyr , Breus Svitlana
Published: February 2021

The study provides solutions for the scientific task related to the improvement of theoretical and development of methodological and applied principles, and the identification and evaluation of risks and threats as factors of anti-crisis management of the enterprises. Based on the developed concept of quantitative risk analysis, we constructed a fuzzy hierarchical model, which gives the possibility to get the estimates: risk factors; specific types of threats in the framework of a process; risk processes, identified in the anti-crisis management; and the integrative risk of anti-crisis management. Furthermore, the proposed model makes it possible to identify the threats that are the risks of the highest (catastrophe) layer. The fuzzy hierarchical model construction process includes the determination of linguistic variables, term-varieties, and universal sets for quantitative evaluation of figures and risks, the establishment of parameters of the membership functions for indicators and risks, the formation of fuzzy knowledge bases, the construction of a fuzzy hierarchical model in the MATLAB environment, the evaluation of adequacy of model based on the learning sample, the correction of a model, and the adoption of a resolution regarding its final variant. The use of the model in the anti-crisis enterprise management will provide the anti-crisis team with the possibility to give early warning of all negative factors, give their quantitative evaluation, and take them into account in the course of making managerial decisions.
pH-Sensitive Dairy-Derived Hydrogels with a Prolonged Drug Release Profile for Cancer Treatment
Authors: Mayorova Oksana A. , Jolly Ben C. N. , Verkhovskii Roman A. , Plastun Valentina O. , Sindeeva Olga A. , Douglas Timothy E. L.
Published: February 2021

A novel versatile biocompatible hydrogel of whey protein isolate (WPI) and two types of tannic acid (TAs) was prepared by crosslinking of WPI with TAs in a one-step method at high temperature for 30 min. WPI is one common protein-based preparation which is used for hydrogel formation. The obtained WPI-TA hydrogels were in disc form and retained their integrity after sterilization by autoclaving. Two TA preparations of differing molecular weight and chemical structure were compared, namely a polygalloyl glucose-rich extract-ALSOK 02-and a polygalloyl quinic acid-rich extract-ALSOK 04. Hydrogel formation was observed for WPI solutions containing both preparations. The swelling characteristics of hydrogels were investigated at room temperature at different pH values, namely 5, 7, and 9. The swelling ability of hydrogels was independent of the chemical structure of the added TAs. A trend of decrease of mass increase (MI) in hydrogels was observed with an increase in the TA/WPI ratio compared to the control WPI hydrogel without TA. This dependence (a MI decrease-TA/WPI ratio) was observed for hydrogels with different types of TA both in neutral and acidic conditions (pH 5.7). Under alkaline conditions (pH 9), negative values of swelling were observed for all hydrogels with a high content of TAs and were accompanied by a significant release of TAs from the hydrogel network. Our studies have shown that the release of TA from hydrogels containing ALSOK04 is higher than from hydrogels containing ALSOK 02. Moreover, the addition of TAs, which display a strong anti-cancer effect, increases the cytotoxicity of WPI-TAs hydrogels against the Hep-2 human laryngeal squamous carcinoma (Hep-2 cells) cell line. Thus, WPI-TA hydrogels with prolonged drug release properties and cytotoxicity effect can be used as anti-cancer scaffolds.
Effect of Nano-Filled Protective Coating and Different pH Enviroment on Wear Resistance of New Glass Hybrid Restorative Material
Authors: Brkanović Sandra , Ivanišević Ana , Miletić Ivana , Mezdić Dražen , Jukić Krmek Silvana
Published: February 2021

The purpose of the study was to determine the wear rate of Equia Forte HT Fil with Equia Forte Coat or without coating and compare it with Fuji IX GP high-viscosity glass ionomer cement (GIC) in conditions with acid load or at neutral pH. The samples were stored for 7 days: (1) in artificial saliva, (2) in artificial saliva and cyclically exposed to low pH, and (3) in distilled water and cyclically exposed to low pH. Wear was determined by measuring the difference in mass before and after brushing in an abrasion testing device. The wear of Fuji IX GP was significantly higher than that of Equia Forte HT Fil with or without coating (p = 0.000). The difference between Equia Forte HT Fil with and without Coat was not statistically significant (p < 0.803). The differences in wear resistance between samples stored in saliva and in distilled water were not significant (p = 0.588). Periodic exposure to the low pH solution significantly affected the wear resistance of all materials (p = 0.000). Equia Forte HT Fil was more resistant to wear than Fuji IX GP in all storage conditions. A resinous coat did not significantly increase wear resistance.
Comparison of Material Properties of Multilayered Laminates Determined by Testing and Micromechanics
Authors: Kulpa Maciej , Wiater Agnieszka , Rajchel Mateusz , Siwowski Tomasz
Published: February 2021

This paper presents an experimental material campaign focusing on fiber-reinforced polymers (FRP) to be applied in a novel bridge deck panel. Laminas based on most commonly used fibers, i.e., glass, carbon, basalt and aramid, were prepared and studied in tension, shear and compression. In the subsequent test stages, different fabric reinforcements (uni- and bi-directional fabrics, woven fabrics, CSM layers) were considered for glass laminas only, and finally, a resultant laminate was designed and tested. Such an approach gives a great opportunity to create “tailor-made” laminates, as required in FRP bridge deck panels. Simultaneously with the laboratory tests, analytical calculations were performed using a few micromechanical models that aimed to determine engineering constants and strength parameters. Then, the results obtained from material testing and analytical calculations were compared, and conclusions on the compliance were drawn. Based on this validation, further analytical calculations may replace time-consuming laboratory tests and facilitate FRP deck design.
Formation of Phases and Microstructures in Al-8Si Alloys with Different Mg Content
Authors: Tian Ni , Wang Guangdong , Zhou Yiran , Liu Chuncheng , Liu Kun , Zhao Gang , Zuo Liang
Published: February 2021

Mg-containing high-silicon aluminum alloy is a heat-treatable aluminum alloy that is now widely used in the aerospace and automotive industries because of its high specific strength, high wear resistance and corrosion resistance, low thermal expansion coefficient, and low cost. More attention has been paid to optimizing the microstructure to increase the performance of this type of aluminum alloy. In the present work, the solidification processes of Mg-free and Mg-containing (0.33–1.32%) Al-8Si alloys were analyzed by the experimental results combined with the thermodynamic calculation. The results showed that α-Al, Si, and Al5FeSi were in the Mg-free Al-8Si alloy ingots, while the Al5FeSi phases in alloys with Mg additions were transformed into π phases (Al8Mg3FeSi6) by the reaction L+Al5FeSi→α-Al+Si+Al8Mg3FeSi6. There was a binary eutectic reaction of L→α-Al+Al5FeSi when the Mg content exceeded 0.51% and the Fe content was higher than 0.17%. With the increase of Mg content, the volume of Mg2Si was gradually increased while the divorced eutectic phenomenon of the quaternary eutectic structure (α-Al+Si+Mg2Si+Al8Mg3FeSi6) was weakened and the eutectic structure was significantly refined.
The Chosen Aspects of Skew Rolling of Hollow Stepped Shafts
Authors: Bartnicki Jarosław , Xia Yingxiang , Shu Xuedao
Published: February 2021

The paper presents chosen aspects of the skew rolling process of hollow stepped products with the use of a skew rolling mill designed and manufactured at the Lublin University of Technology. This machine is characterized by the numerical control of spacing between the working rolls and the sequence of the gripper axial movement, which allows for the individual programming of the obtained shapes of parts such as stepped axles and shafts. The length of these zones and the values of possibly realizable cross-section reduction and obtained outlines are the subject of this research paper. The chosen results regarding the influence of the technological parameters used on the course of the process are shown in the present study. Numerical modelling using the finite element method in Simufact Forming, as well as the results of experimental tests performed in a skew rolling mill, were applied in the conducted research. The work takes into account the influence of cross-section reduction of the hollow parts and the feed rate per rotation on the metal flow mechanisms in the skew rolling process. The presented results concern the obtained dimensional deviations and changes in the wall thickness determining the proper choice of technological parameters for hollow parts formed by the skew rolling method. Knowledge about the cause of the occurrence of these limitations is very important for the development of this technology and the choice of the process parameters.
Low Recombination Firing-Through Al Paste for N-Type Solar Cell with Boron Emitter
Authors: Zhu Peng , Liu Yuan , Cao Chengjiang , Tian Juan , Zhang Aichuang , Wang Deliang
Published: February 2021

A kind of low recombination firing-through screen-printing aluminum (Al) paste is proposed in this work to be used for a boron-diffused N-type solar cell front side metallization. A front side fire-through contact (FTC) approach has been carried out for the formation of local contacts for a front surface passivated solar cell. With a low contact resistivity (ρc) of 1.0 mΩ·cm2, good ohmic contact between the boron-doped front surface of the silicon sample and the Al paste was realized. To obtain a good energy conversion efficiency, a balance can be achieved between the open circuit voltage (Voc) and contact resistivity (ρc) of the cell by combining suitable Al powders and appropriate additives. The detailed micro-contact difference in Si/metallization between the firing-through Al paste and silver-aluminum (Ag-Al) paste was analyzed. The dark saturation current density beneath the metal contact (J0, metal) of the Si/metallization region using our firing-through Al paste was discussed, which was proven to be 61% lower than using Ag-Al paste. The pseudo energy conversion efficiency of the cell using Al paste measured by Suns-VOC was also higher than using Ag-Al paste. The role of Al paste in low surface metal recombination is discussed. The utilization of this new kind of Al paste was much cheaper and more convenient, compared to the traditional process using Ag or Ag-Al paste.
Analytical Method for Mechanism Design in Partially Observable Markov Games
Authors: Clempner Julio B. , Poznyak Alexander S.
Published: February 2021

A theme that become common knowledge of the literature is the difficulty of developing a mechanism that is compatible with individual incentives that simultaneously result in efficient decisions that maximize the total reward. In this paper, we suggest an analytical method for computing a mechanism design. This problem is explored in the context of a framework, in which the players follow an average utility in a non-cooperative Markov game with incomplete state information. All of the Nash equilibria are approximated in a sequential process. We describe a method for the derivative of the player’s equilibrium that instruments the design of the mechanism. In addition, it showed the convergence and rate of convergence of the proposed method. For computing the mechanism, we consider an extension of the Markov model for which it is introduced a new variable that represents the product of the mechanism design and the joint strategy. We derive formulas to recover the variables of interest: mechanisms, strategy, and distribution vector. The mechanism design and equilibrium strategies computation differ from those in previous literature. A numerical example presents the usefulness and effectiveness of the proposed method.
Prevalence of Food-Hypersensitivity and Food-Dependent Anaphylaxis in Colombian Schoolchildren by Parent-Report
Authors: Beltrán-Cárdenas Carlos Eduardo , Granda-Restrepo Diana María , Franco-Aguilar Alejandro , Lopez-Teros Veronica , Arvizu-Flores Aldo Alejandro , Cárdenas-Torres Feliznando Isidro , Ontiveros Noé , Cabrera-Chávez Francisco , Arámburo-Gálvez Jesús Gilberto
Published: February 2021

Background and objectives: The epidemiology of food allergy (FA) and food-dependent anaphylaxis remains unknown in Colombia. Our aim was to estimate by parent-report the prevalence of FA and food-dependent anaphylaxis in a Colombian population of schoolchildren. Materials and methods: A printed questionnaire was sent to parents of schoolchildren aged 5–12 years old from Medellín, Colombia in order to collect FA-related data. Results: Nine hundred and sixty-nine (969) parents returned the questionnaire with valid responses (response rate, 52.5%). The estimated prevalence rates (95% CI) were: adverse food reactions 12.79% (10.76–15.07), “perceived FA, ever” 10.93% (9.08–13.08), “physician-diagnosed FA, ever” 4.33% (3.14–5.81), “immediate-type FA, ever” 6.81% (5.30–8.58), “immediate-type FA, current” 3.30% (2.26–4.63), and food-dependent anaphylaxis 1.85% (1.10–2.92). The most frequently reported food allergens were milk (1.44%), fruits (0.41%), meat (0.41%), and peanut (0.3%). Sixty-one percent of “food-dependent anaphylaxis” cases sought medical attention, but only eleven percent of the cases reported the prescription of an epinephrine autoinjector. Conclusions: FA and food-dependent anaphylaxis are not uncommon among schoolchildren from Colombia. The prescription of epinephrine autoinjectors should be encouraged among health personnel for the optimal management of suspected cases of food-dependent anaphylaxis.
PLGA Multiplex Membrane Platform for Disease Modelling and Testing of Therapeutic Compounds
Authors: Piscioneri Antonella , Morelli Sabrina , Drioli Enrico , De Bartolo Loredana
Published: February 2021

A proper validation of an engineered brain microenvironment requires a trade of between the complexity of a cellular construct within the in vitro platform and the simple implementation of the investigational tool. The present work aims to accomplish this challenging balance by setting up an innovative membrane platform that represents a good compromise between a proper mimicked brain tissue analogue combined with an easily accessible and implemented membrane system. Another key aspect of the in vitro modelling disease is the identification of a precise phenotypic onset as a definite hallmark of the pathology that needs to be recapitulated within the implemented membrane system. On the basis of these assumptions, we propose a multiplex membrane system in which the recapitulation of specific neuro-pathological onsets related to Alzheimer’s disease pathologies, namely oxidative stress and β-amyloid1–42 toxicity, allowed us to test the neuroprotective effects of trans-crocetin on damaged neurons. The proposed multiplex membrane platform is therefore quite a versatile tool that allows the integration of neuronal pathological events in combination with the testing of new molecules. The present paper explores the use of this alternative methodology, which, relying on membrane technology approach, allows us to study the basic physiological and pathological behaviour of differentiated neuronal cells, as well as their changing behaviour, in response to new potential therapeutic treatment.
Novel Technique for Enhancing the Strength of Friction Stir Spot Welds through Dynamic Welding Parameters
Authors: Badwelan Ahmed , M. Al-Samhan Ali , Anwar Saqib , Hidri Lotfi
Published: February 2021

Presently, friction stir spot welding (FSSW) has become a common alternative for spot welding technologies. Over the years, researchers have implemented various methods for enhancing weld strength. However, the literature shows that the previously reported approaches have used static (constant) welding parameters set at the beginning of the welding stroke (i.e., the FSSW parameters were kept constant during the welding stroke). In contrast, in this study, an innovative technique is proposed for enhancing the weld strength for Al 1050 material by adjusting the FSSW process parameters during the welding stroke. Two FSSW parameters, namely, feed rate and spindle speed (dynamic parameters), are used in this study with a stepwise variation function and are changed during the welding stroke. The results of this study show that the weld tensile strength is enhanced by 12–21% when using the proposed novel dynamic welding parameter technique. This is a significant increase in the weld strength compared to when static welding parameters are employed during the welding stroke.
Enhancement of Efficiency of Pd/Al2O3Catalysts in Selective Hydrogenation of Sec-Butylbenzene by Modification with H2SO4 or H2WO4
Authors: Kustov Leonid M. , Tarasov Andrei L. , Kustov Alexander L. , Tkachenko Olga P.
Published: February 2021

Hydrogenation of bulky aromatic hydrocarbons is an important problem to be solved in order to improve the quality of fuels. Pd-containing catalysts modified by strong acids have been prepared and studied by diffuse-reflectance IR spectroscopy, and the catalytic activity of the materials has been determined. In studying the selective liquid-phase hydrogenation of sec-butylbenzene as a model substrate, it was shown that modification of Pd/Al2O3 catalysts with acid additives (H2SO4 or H2WO4) results in a significant increase in the hydrogenation activity and selectivity. IR spectroscopy of adsorbed CO and d3-acetonitrile revealed that larger palladium metal particles are formed on the Pd-H2SO4(H2WO4)/Al2O3 catalysts, with ionic states of palladium being present even in the samples reduced in H2.
Carotenoids and Some Other Pigments from Fungi and Yeasts
Authors: Rapoport Alexander , Guzhova Irina , Bernetti Lorenzo , Buzzini Pietro , Kieliszek Marek , Kot Anna Maria
Published: February 2021

Carotenoids are an essential group of compounds that may be obtained by microbiological synthesis. They are instrumental in various areas of industry, medicine, agriculture, and ecology. The increase of carotenoids’ demand at the global market is now essential. At the moment, the production of natural carotenoids is more expensive than obtaining their synthetic forms, but several new approaches/directions on how to decrease this difference were developed during the last decades. This review briefly describes the information accumulated until now about the beneficial effects of carotenoids on human health protection, their possible application in the treatments of various diseases, and their use in the food and feed industry. This review also describes some issues that are linked with biotechnological production of fungal and yeasts carotenoids, as well as new approaches/directions to make their biotechnological production more efficient.
Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP
Authors: Becherer Lisa , Hess Jacob Friedrich , Frischmann Sieghard , Bakheit Mohammed , Nitschko Hans , Stinco Silvina , Zitz Friedrich , Hofer Hannes , Porro Giampiero , Hausladen Florian , Stock Karl , Drossart Dominik , Wurm Holger , Kuhn Hanna , Huber Dominik , Hutzenlaub Tobias , Paust Nils , Keller Mark , Strohmeier Oliver , Wadle Simon , Borst Nadine , Zengerle Roland , von Stetten Felix
Published: February 2021

This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD LAMP (mediator displacement loop-mediated isothermal amplification). A disposable microfluidic LabDisk with pre-stored reagents performs automated nucleic acid extraction, reaction setup, emulsification, reverse transcription, digital DNA amplification, and quantitative fluorogenic endpoint detection with universal reporter molecules. Automated nucleic acid extraction from a suspension of HTLV-1-infected CD4+ T-lymphocytes (MT-2 cells) yielded 8 ± 7 viral nucleic acid copies per MT-2 cell, very similar to the manual reference extraction (7 ± 2 nucleic acid copies). Fully automated sample processing from whole blood spiked with MT-2 cells showed a comparable result of 7 ± 3 copies per MT-2 cell after a run time of two hours and 10 min.