bluuebunny/crossref_metadata_2025 huggingface dataset:
The Comparative Toxicity, Biochemical and Physiological Impacts of Chlorantraniliprole and Indoxacarb on Mamestra brassicae (Lepidoptera: Noctuidae)
Authors: Moustafa Moataz A. M. , Fouad Eman A. , Ibrahim Emad , Erdei Anna Laura , Kárpáti Zsolt , Fónagy Adrien
Published: February 2023

Background: The cabbage moth, Mamestra brassicae, is a polyphagous pest that attacks several crops. Here, the sublethal and lethal effects of chlorantraniliprole and indoxacarb were investigated on the developmental stages, detoxification enzymes, reproductive activity, calling behavior, peripheral physiology, and pheromone titer of M. brasssicae. Methods: To assess pesticide effects, the second instar larvae were maintained for 24 h on a semi-artificial diet containing insecticides at their LC10, LC30, and LC50 concentrations. Results: M. brassicae was more susceptible to chlorantraniliprole (LC50 = 0.35 mg/L) than indoxacarb (LC50 = 1.71 mg/L). A significantly increased developmental time was observed with both insecticides at all tested concentrations but decreases in pupation rate, pupal weight, and emergence were limited to the LC50 concentration. Reductions in both the total number of eggs laid per female and the egg viability were observed with both insecticides at their LC30 and LC50 concentrations. Both female calling activity and the sex pheromone (Z11-hexadecenyl acetate and hexadecenyl acetate) titer were significantly reduced by chlorantraniliprole in LC50 concentration. Antennal responses of female antennae to benzaldehyde and 3-octanone were significantly weaker than controls after exposure to the indoxocarb LC50 concentration. Significant reductions in the enzymatic activity of glutathione S-transferases, mixed-function oxidases, and carboxylesterases were observed in response to both insecticides.
Toxicity of Difenoconazole and Atrazine and Their Photodegradation Products on Aquatic Biota: Environmental Implications in Countries Lacking Good Agricultural Practices
Authors: Mendieta Herrera Julia , Iñiguez Armijos Carlos , Rosado Alcarria Daniel , Aguilar Ramírez Silvio
Published: February 2023

Agriculture is fundamental for human development, but it may also have a range of unwanted effects on ecosystems when pesticides inadvertently enter the environment. We determined the toxicity of difenoconazole and atrazine, as well as their photodegradation products, on the bioindicators Lemna minor and Daphnia magna. For L. minor, we assessed the number of leaves, biomass, and chlorophyll content exposed to different concentrations of difenoconazole (0–8 mg/L) and atrazine (0–3.84 mg/L). For D. magna, we assessed the mortality to difenoconazole (0–1.6 mg/L) and atrazine (0–80 mg/L). We found that the higher the concentrations of the pesticides, the higher the toxicity for both bioindicators. In L. minor, the highest toxicity for atrazine was 0.96 mg/L, whereas for difenoconazole, it was 8 mg/L. For D. magna, the 48 h LC50 for difenoconazole was 0.97 mg/L, while for atrazine, it was 86.19 mg/L. For L. minor, the toxicity of difenoconazole and atrazine was not different compared to that of their photodegradation products. In contrast, for D. magna, difenoconazole, but not atrazine, was more toxic compared to its respective photodegradation products. Pesticides are a serious threat to aquatic biota, and their photodegradation products remain toxic in the environment. Additionally, the use of bioindicators can help monitor these pollutants in aquatic ecosystems in countries where the application of pesticides is imperative for agricultural production.
Gestational and Lactational Co-Exposure to DEHP and BPA Impairs Hepatic Function via PI3K/AKT/FOXO1 Pathway in Offspring
Authors: Wang Minghan , Wang Yu , Han Junyuan , Duan Zhiwen , Yin Jiye , Ding Rigao , Wang Quanjun
Published: February 2023

Di-(2-Ethylhexyl) phthalate (DEHP) and bisphenol A (BPA) present significant environmental endocrine-disrupting chemical properties. Although studies have implied reproductive impairment from exposure to BPA and DEHP, no study to date has shown the effect and mechanism of hepatic function after gestational and lactational co-exposure to DEHP and BPA in offspring. A total of 36 perinatal rats were randomly divided into four groups, DEHP (600 mg/kg/day), BPA (80 mg/kg/day), DEHP combined with BPA (600 mg/kg/day + 80 mg/kg/day), and control. Notably, 11 chemical targets were screened after identifying eight substances associated with chemically-induced hepatic damage. Molecular docking simulations revealed a high-scoring combination of eight metabolic components and targets of the PI3K/AKT/FOXO1 signaling pathway. The DEHP and BPA combination disrupted hepatic steatosis, ultimately affecting systemic the glucose and the lipid metabolic homeostasis with significant toxicity. Mechanistically, co-exposure to DEHP and BPA causes liver dysfunction and hepatic insulin resistance via PI3K/AKT/FOXO1 pathway in offspring. This is the first study of the hepatic function and mechanism of co-exposure to DEHP and BPA that combines metabolomics, molecular docking, and traditional toxicity assessment methods.
Residues and Safety Assessment of Cyantraniliprole and Indoxacarb in Wild Garlic (Allium vineale)
Authors: Sardar Syed Wasim , Choi Jeong Yoon , Jo Yeong Ju , Ishag Abd Elaziz Sulieman Ahmed , Kim Min-woo , Ham Hun Ju
Published: February 2023

In this study, the residual behavior and safety of cyantraniliprole and indoxacarb applied to wild garlic (Allium vineale) were investigated. Samples were harvested after treatments of 0, 3, 7, and 14 days, then were prepared and extracted following the QuEChERS method and analyzed by UPLC-MS/MS. The linearity (R2 ≥ 0.99) of the calibration curves was excellent for both compounds. The average recoveries of cyantraniliprole and indoxacarb at two spiking concentrations (0.01 and 0.1 mg/kg) ranged from 94.2% to 111.4%. The relative standard deviation value was below 10%. The initial concentrations of cyantraniliprole and indoxacarb in wild garlic were degraded to 75% and 93% after seven days. The average half-lives were 1.83 and 1.14 days for cyantraniliprole and indoxacarb, respectively. The preharvest intervals (PHIs) for the two pesticides in wild garlic are recommended as two treatments seven days before harvest. The safety assessment data indicated that the percent acceptable daily intakes of cyantraniliprole and indoxacarb were 0.3 × 10−4% and 6.7 × 10−2%, respectively, in wild garlic. The theoretical maximum daily intake value of cyantraniliprole was 9.80%, and that of indoxacarb was 60.54%. Both compounds’ residues in wild garlic pose low health risks to consumers. The findings of the current investigation provide essential data for the safe use of cyantraniliprole and indoxacarb in wild garlic.
The Migration Rules of Malathion during Indoor Simulated Lake Freezing
Authors: Zhang Yan , Wang Xiaozhuang , Zhao Wanli , Liu Yucan , Liu Tongshuai , Yang Peiyuan
Published: February 2023

The effect of malathion in ice is a poorly researched area, and ice is an important habitat for organisms at the base of the food web. This study presents laboratory-controlled experiments designed to investigate the migration rule of malathion during lake freezing. Concentrations of malathion were determined in samples of melted ice and in under-ice water. The effects of the initial sample concentration, freezing ratio, and freezing temperature on the distribution of malathion in the ice–water system were investigated. The concentration effect and migration capacity of malathion during freezing was characterized by the concentration rate and distribution coefficient. The results showed that the formation of ice led to the concentration of malathion appearing as follows: concentration in under-ice water > concentration in raw water > concentration in ice. This implied that malathion tended to migrate from the ice to the under-ice water during the freezing process. The increase in the initial malathion concentration, freezing ratio, and freezing temperature caused a more pronounced repulsion of the malathion by the ice and increased the migration to the under-ice water. When the solution of malathion with an initial concentration of 50 μg/L was frozen at –9 °C and the freezing ratio reached 60%, the concentration of malathion in the under-ice water was concentrated to 2.34 times the initial concentration. The migration of malathion to under-ice water during freezing may pose a potential threat to under-ice ecology; therefore, the environmental quality and impact of under-ice water in icebound lakes needs to be given more attention.
Abstracts of Presentations to the Working Session on Improving Predictive Modeling of Mycotoxin Risk for Africa Held at the 3rd ASM2022 on 7 September 2022, in Stellenbosch, South Africa
Authors: Rembold Felix , Mvumi Brighton , Miller David , Omari Rose , Battilani Paola , Galani Yamdeu Joseph Hubert , Louw Wiana , Falade Titilayo D. O. , Schweiger Wolfgang , Ermolli Monica
Published: February 2023

In 2008, the African Postharvest Losses Information Systems project (APHLIS, accessed on 6 September 2022) developed an algorithm for estimating the scale of cereal postharvest losses (PHLs). The relevant scientific literature and contextual information was used to build profiles of the PHLs occurring along the value chains of nine cereal crops by country and province for 37 sub-Saharan African countries. The APHLIS provides estimates of PHL figures where direct measurements are not available. A pilot project was subsequently initiated to explore the possibility of supplementing these loss estimates with information on the aflatoxin risk. Using satellite data on drought and rainfall, a time series of agro-climatic aflatoxin risk warning maps for maize was developed covering the countries and provinces of sub-Saharan Africa. The agro-climatic risk warning maps for specific countries were shared with mycotoxin experts from those countries for review and comparison with their aflatoxin incidence datasets. The present Work Session was a unique opportunity for African food safety mycotoxins experts, as well as other international experts, to meet and deepen the discussion about prospects for using their experience and their data to validate and improve agro-climatic risk modeling approaches.
Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography
Authors: Endre Gábor , Nagy Babett Edit , Hercegfalvi Dániel , Kasuba Csenge , Vágvölgyi Csaba , Szekeres András
Published: February 2023

Aflatoxins (AFs) are a group of secondary metabolites that cause several diseases in both animals and humans. Since the discovery of this group of toxins, several effects were revealed, such as hepatic changes, carcinoma, failure, and cancer of the liver. In the European Union, there are concentration limits for this group of mycotoxins in food and feed products; thus, these substances are required in their pure forms to prepare reference standards or certified reference materials. In our present work, a liquid–liquid chromatographic method utilizing a toluene/acetic acid/water ternary system was improved. In order to enhance the purification and gain a higher amount of pure AFs in one separation run, a scale-up of the previous separation was carried out. In several scale-up steps—including the determination of the maximum concentration and volume to load on a 250 mL rotor via a loop and via a pump as well, and the quadruplication of the entire separation procedure to a 1000 mL rotor—an efficient scale-up was achieved. Utilizing a 250 mL rotor in an 8-hour workday, altogether approximately 2.2 g of total AFs could be purified with 8.2 liters of solvent, while on a 1000 mL column, approximately 7.8 g AFs could be prepared, utilizing around 31 liters of solvents.
Advanced Oxidation Protein Products Contribute to Chronic-Kidney-Disease-Induced Adipose Inflammation through Macrophage Activation
Authors: Arimura Nanaka , Watanabe Hiroshi , Kato Hiromasa , Imafuku Tadashi , Nakano Takehiro , Sueyoshi Miyu , Chikamatsu Mayuko , Tokumaru Kai , Nagasaki Taisei , Maeda Hitoshi , Tanaka Motoko , Matsushita Kazutaka , Maruyama Toru
Published: February 2023

Fat atrophy and adipose tissue inflammation can cause the pathogenesis of metabolic symptoms in chronic kidney disease (CKD). During CKD, the serum levels of advanced oxidation protein products (AOPPs) are elevated. However, the relationship between fat atrophy/adipose tissue inflammation and AOPPs has remained unknown. The purpose of this study was to investigate the involvement of AOPPs, which are known as uremic toxins, in adipose tissue inflammation and to establish the underlying molecular mechanism. In vitro studies involved co-culturing mouse-derived adipocytes (differentiated 3T3-L1) and macrophages (RAW264.7). In vivo studies were performed using adenine-induced CKD mice and AOPP-overloaded mice. Fat atrophy, macrophage infiltration and increased AOPP activity in adipose tissue were identified in adenine-induced CKD mice. AOPPs induced MCP-1 expression in differentiated 3T3-L1 adipocytes via ROS production. However, AOPP-induced ROS production was suppressed by the presence of NADPH oxidase inhibitors and the scavengers of mitochondria-derived ROS. A co-culturing system showed AOPPs induced macrophage migration to adipocytes. AOPPs also up-regulated TNF-α expression by polarizing macrophages to an M1-type polarity, and then induced macrophage-mediated adipose inflammation. In vitro data was supported by experiments using AOPP-overloaded mice. AOPPs contribute to macrophage-mediated adipose inflammation and constitute a potential new therapeutic target for adipose inflammation associated with CKD.
Streptavidin-Saporin: Converting Biotinylated Materials into Targeted Toxins
Authors: Ancheta Leonardo R. , Shramm Patrick A. , Bouajram Raschel , Higgins Denise , Lappi Douglas A.
Published: February 2023

Streptavidin-Saporin can be considered a type of ‘secondary’ targeted toxin. The scientific community has taken advantage of this conjugate in clever and fruitful ways using many kinds of biotinylated targeting agents to send saporin into a cell selected for elimination. Saporin is a ribosome-inactivating protein that causes inhibition of protein synthesis and cell death when delivered inside a cell. Streptavidin-Saporin, mixed with biotinylated molecules to cell surface markers, results in powerful conjugates that are used both in vitro and in vivo for behavior and disease research. Streptavidin-Saporin harnesses the ‘Molecular Surgery’ capability of saporin, creating a modular arsenal of targeted toxins used in applications ranging from the screening of potential therapeutics to behavioral studies and animal models. The reagent has become a well-published and validated resource in academia and industry. The ease of use and diverse functionality of Streptavidin-Saporin continues to have a significant impact on the life science industry.
Prevalence of Hepatitis B Virus Infection among Inmates at the Monrovia Central Prison, Liberia
Authors: Vessellee David B. , Yalley Akua K. , Adjei David N. , Appeaning Mark , Odoom Prince N. , Kyeremeh Ransford , Yalley Abena Asefuaba , Dahn Bernice , Nii-Trebi Nicholas I.
Published: February 2023

Determination of hepatitis B virus (HBV) infections in key populations including prison inmates is crucial for formulating appropriate intervention approaches. However, in many low-income countries, such as Liberia, there is hardly any documentation on HBV prevalence among inmates. This study determined and evaluated the prevalence of HBV infections among incarcerated persons in the Monrovia Central Prison, Liberia. One hundred participants comprising 76 males and 24 females were studied. Participants’ demographic and potential risk factors information were obtained using a semi-structured questionnaire, and blood samples were collected for the analysis. Plasma was tested for five HBV serological markers, namely, HBsAg, HBsAb, HBeAg, HBeAb, and HBcAb. The seroreactivity of actively infected persons was confirmed by nucleic acid detection. Results of the serological assay showed that 34% of the participants had been exposed to the virus and 14% were actively infected. qPCR confirmed HBV DNA in seven actively infected samples. Statistical analysis indicated that a low level of education, a history of blood transfusion, and intravenous drug use, were significant predictors of active HBV infection and HBV exposure, respectively. These findings might make the testing and vaccination of convicts against HBV infection prior to their admission into prison facilities imperative.
Geographical Influence on Morphometric Variability of Genetically “Pure” Schistosoma haematobium Eggs from Sub-Saharan Migrants in Spain
Authors: Reguera-Gómez Marta , Valero Maria Adela , Artigas Patricio , De Elías-Escribano Alejandra , Fantozzi Maria Cecilia , Luzón-García Maria Pilar , Salas-Coronas Joaquín , Boissier Jérôme , Mas-Coma Santiago , Bargues Maria Dolores
Published: February 2023

Schistosome eggs play a key role in schistosomiasis diagnosis and research. The aim of this work is to morphogenetically study the eggs of Schistosoma haematobium found in sub-Saharan migrants present in Spain, analyzing their morphometric variation in relation to the geographical origin of the parasite (Mali, Mauritania and Senegal). Only eggs considered “pure” S. haematobium by genetic characterization (rDNA ITS-2 and mtDNA cox1) have been used. A total of 162 eggs obtained from 20 migrants from Mali, Mauritania and Senegal were included in the study. Analyses were made by the Computer Image Analysis System (CIAS). Following a previously standardized methodology, seventeen measurements were carried out on each egg. The morphometric analysis of the three morphotypes detected (round, elongated and spindle) and the biometric variations in relation to the country of origin of the parasite on the egg phenotype were carried out by canonical variate analysis. Mahalanobis distances, when all egg measurements were analyzed, showed differences between: (i) Mali-Mauritania, Mali-Senegal and Mauritania-Senegal in the round morphotype; (ii) Mali-Mauritania and Mauritania-Senegal in the elongated morphotype; and (iii) Mauritania-Senegal in the spindle morphotype. Mahalanobis distances, when spine variables were analyzed, showed differences between Mali-Senegal in the round morphotype. In conclusion, this is the first phenotypic study performed on individually genotyped “pure” S. haematobium eggs, allowing the assessment of the intraspecific morphological variations associated with the geographical origin of the schistosome eggs.
An Analytical Approach to Bayesian Evidence Computation
Authors: García-Bellido Juan
Published: February 2023

Bayesian evidence is a key tool in model selection, allowing a comparison of models with different numbers of parameters. Its use in the analysis of cosmological models has been limited by difficulties in calculating it, with current numerical algorithms requiring supercomputers. In this paper we give exact formulae for the Bayesian evidence in the case of Gaussian likelihoods with arbitrary correlations and top-hat priors, and approximate formulae for the case of likelihood distributions with leading non-Gaussianities (skewness and kurtosis). We apply these formulae to cosmological models with and without isocurvature components, and compare with results we previously obtained using numerical thermodynamic integration. We find that the results are of lower precision than the thermodynamic integration, while still being good enough to be useful.
The Kinetics of Humoral and Cellular Responses after the Booster Dose of COVID-19 Vaccine in Inflammatory Arthritis Patients
Authors: Wroński Jakub , Jaszczyk Bożena , Roszkowski Leszek , Felis-Giemza Anna , Bonek Krzysztof , Kornatka Anna , Plebańczyk Magdalena , Burakowski Tomasz , Lisowska Barbara , Kwiatkowska Brygida , Maśliński Włodzimierz , Wisłowska Małgorzata , Massalska Magdalena , Kuca-Warnawin Ewa , Ciechomska Marzena
Published: February 2023

Impaired immunogenicity of COVID-19 vaccinations in inflammatory arthritis (IA) patients results in diminished immunity. However, optimal booster vaccination regimens are still unknown. Therefore, this study aimed to assess the kinetics of humoral and cellular responses in IA patients after the COVID-19 booster. In 29 IA patients and 16 healthy controls (HC), humoral responses (level of IgG antibodies) and cellular responses (IFN-γ production) were assessed before (T0), after 4 weeks (T1), and after more than 6 months (T2) from the booster vaccination with BNT162b2. IA patients, but not HC, showed lower anti-S-IgG concentration and IGRA fold change at T2 compared to T1 (p = 0.026 and p = 0.031). Furthermore, in IA patients the level of cellular response at T2 returned to the pre-booster level (T0). All immunomodulatory drugs, except IL-6 and IL-17 inhibitors for the humoral and IL-17 inhibitors for the cellular response, impaired the immunogenicity of the booster dose at T2. Our study showed impaired kinetics of both humoral and cellular responses after the booster dose of the COVID-19 vaccine in IA patients, which, in the case of cellular response, did not allow the vaccination effect to be maintained for more than 6 months. Repetitive vaccination with subsequent booster doses seems to be necessary for IA patients.
Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China
Authors: Zhao Guanyu , Gao Yan , Shi Ning , Zhang Shiheng , Xiao Pengpeng , Zhang Jiaqi , Xie Changzhan , Ha Zhuo , Feng Sheng , Li Chenghui , Zhang Xuancheng , Xie Yubiao , Yu Ning , Zhang He , Bi Junlong , Jin Ningyi
Published: February 2023

Japanese encephalitis virus (JEV), which uses a mosquito primary vector and swine as a reservoir host, poses a significant risk to human and animal health. JEV can be detected in cattle, goats and dogs. A molecular epidemiological survey of JEV was conducted in 3105 mammals from five species, swine, fox, racoon dog, yak and goat, and 17,300 mosquitoes from 11 Chinese provinces. JEV was detected in pigs from Heilongjiang (12/328, 3.66%), Jilin (17/642, 2.65%), Shandong (14/832, 1.68%), Guangxi (8/278, 2.88%) and Inner Mongolia (9/952, 0.94%); in goats (1/51, 1.96%) from Tibet; and mosquitoes (6/131, 4.58%) from Yunnan. A total of 13 JEV envelope (E) gene sequences were amplified in pigs from Heilongjiang (5/13), Jilin (2/13) and Guangxi (6/13). Swine had the highest JEV infection rate of any animal species, and the highest infection rates were found in Heilongjiang. Phylogenetic analysis indicated that the predominant strain in Northern China was genotype I. Mutations were found at residues 76, 95, 123, 138, 244, 474 and 475 of E protein but all sequences had predicted glycosylation sites at ′N154. Three strains lacked the threonine 76 phosphorylation site from non-specific (unsp) and protein kinase G (PKG) site predictions; one lacked the threonine 186 phosphorylation site from protein kinase II (CKII) prediction; and one lacked the tyrosine 90 phosphorylation site from epidermal growth factor receptor (EGFR) prediction. The aim of the current study was to contribute to JEV prevention and control through the characterization of its molecular epidemiology and prediction of functional changes due to E-protein mutations.
Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae
Authors: Obradović Mina , Malešević Milka , Di Luca Mariagrazia , Kekić Dušan , Gajić Ina , McAuliffe Olivia , Neve Horst , Stanisavljević Nemanja , Vukotić Goran , Kojić Milan
Published: February 2023

Klebsiella pneumoniae is a global health threat and bacteriophages are a potential solution in combating pandrug-resistant K. pneumoniae infections. Two lytic phages, LASTA and SJM3, active against several pandrug-resistant, nosocomial strains of K. pneumoniae were isolated and characterized. Their host range is narrow and latent period is particularly long; however, their lysogenic nature was refuted using both bioinformatic and experimental approaches. Genome sequence analysis clustered them with only two other phages into the new genus Lastavirus. Genomes of LASTA and SJM3 differ in only 13 base pairs, mainly located in tail fiber genes. Individual phages, as well as their cocktail, demonstrated significant bacterial reduction capacity in a time-dependent manner, yielding up to 4 log reduction against planktonic, and up to 2.59 log on biofilm-embedded, cells. Bacteria emerging from the contact with the phages developed resistance and achieved numbers comparable to the growth control after 24 h. The resistance to the phage seems to be of a transient nature and varies significantly between the two phages, as resistance to LASTA remained constant while resensitization to SJM3 was more prominent. Albeit with very few differences, SJM3 performed better than LASTA overall; however, more investigation is needed in order to consider them for therapeutic application.
Baseline Plasma Osteopontin Protein Elevation Predicts Adverse Outcomes in Hospitalized COVID-19 Patients
Authors: Tonello Stelvio , D’Onghia Davide , Apostolo Daria , Matino Erica , Costanzo Martina , Casciaro Giuseppe Francesco , Croce Alessandro , Rizzi Eleonora , Zecca Erika , Pedrinelli Anita Rebecca , Vassia Veronica , Ravanini Paolo , Crobu Maria Grazia , Rizzi Manuela , Landi Raffaella , Castello Luigi Mario , Minisini Rosalba , Avanzi Gian Carlo , Pirisi Mario , Lilleri Daniele , Bellan Mattia , Colangelo Donato , Sainaghi Pier Paolo
Published: February 2023

More than three years have passed since the first case, and COVID-19 is still a health concern, with several open issues such as the lack of reliable predictors of a patient’s outcome. Osteopontin (OPN) is involved in inflammatory response to infection and in thrombosis driven by chronic inflammation, thus being a potential biomarker for COVID-19. The aim of the study was to evaluate OPN for predicting negative (death or need of ICU admission) or positive (discharge and/or clinical resolution within the first 14 days of hospitalization) outcome. We enrolled 133 hospitalized, moderate-to-severe COVID-19 patients in a prospective observational study between January and May 2021. Circulating OPN levels were measured by ELISA at admission and at day 7. The results showed a significant correlation between higher plasma concentrations of OPN at hospital admission and a worsening clinical condition. At multivariate analysis, after correction for demographic (age and gender) and variables of disease severity (NEWS2 and PiO2/FiO2), OPN measured at baseline predicted an adverse prognosis with an odds ratio of 1.01 (C.I. 1.0–1.01). At ROC curve analysis, baseline OPN levels higher than 437 ng/mL predicted a severe disease evolution with 53% sensitivity and 83% specificity (area under the curve 0.649, p = 0.011, likelihood ratio of 1.76, (95% confidence interval (CI): 1.35–2.28)). Our data show that OPN levels determined at the admission to hospital wards might represent a promising biomarker for early stratification of patients’ COVID-19 severity. Taken together, these results highlight the involvement of OPN in COVID-19 evolution, especially in dysregulated immune response conditions, and the possible use of OPN measurements as a prognostic tool in COVID-19.
Influence of Canonical and Non-Canonical IFNLR1 Isoform Expression on Interferon Lambda Signaling
Authors: Evans John Grayson , Novotny Laura A. , Meissner Eric G.
Published: February 2023

Interferon lambdas (IFNLs) are innate immune cytokines that induce antiviral cellular responses by signaling through a heterodimer composed of IL10RB and the interferon lambda receptor 1 (IFNLR1). Multiple IFNLR1 transcriptional variants are expressed in vivo and are predicted to encode distinct protein isoforms whose function is not fully established. IFNLR1 isoform 1 has the highest relative transcriptional expression and encodes the full-length functional form that supports canonical IFNL signaling. IFNLR1 isoforms 2 and 3 have lower relative expression and are predicted to encode signaling-defective proteins. To gain insight into IFNLR1 function and regulation, we explored how altering relative expression of IFNLR1 isoforms influenced the cellular response to IFNLs. To achieve this, we generated and functionally characterized stable HEK293T clones expressing doxycycline-inducible FLAG-tagged IFNLR1 isoforms. Minimal FLAG-IFNLR1 isoform 1 overexpression markedly increased IFNL3-dependent expression of antiviral and pro-inflammatory genes, a phenotype that could not be further augmented by expressing higher levels of FLAG-IFNLR1 isoform 1. Expression of low levels of FLAG-IFNLR1 isoform 2 led to partial induction of antiviral genes, but not pro-inflammatory genes, after IFNL3 treatment, a phenotype that was largely abrogated at higher FLAG-IFNLR1 isoform 2 expression levels. Expression of FLAG-IFNLR1 isoform 3 partially augmented antiviral gene expression after IFNL3 treatment. In addition, FLAG-IFNLR1 isoform 1 significantly reduced cellular sensitivity to the type-I IFN IFNA2 when overexpressed. These results identify a unique influence of canonical and non-canonical IFNLR1 isoforms on mediating the cellular response to interferons and provide insight into possible pathway regulation in vivo.
Twenty Years of Collaboration to Sort out Phage Mu Replication and Its Dependence on the Mu Central Gyrase Binding Site
Authors: Toussaint Ariane , Higgins N. Patrick
Published: February 2023

For 20 years, the intricacies in bacteriophage Mu replication and its regulation were elucidated in collaboration between Ariane Toussaint and her co-workers in the Laboratory of Genetics at the Université Libre de Bruxelles, and the groups of Martin Pato and N. Patrick Higgins in the US. Here, to honor Martin Pato’s scientific passion and rigor, we tell the history of this long-term sharing of results, ideas and experiments between the three groups, and Martin’s final discovery of a very unexpected step in the initiation of Mu replication, the joining of Mu DNA ends separated by 38 kB with the assistance of the host DNA gyrase.
Extracellular Vesicle-Based SARS-CoV-2 Vaccine
Authors: Matsuzaka Yasunari , Yashiro Ryu
Published: February 2023

Messenger ribonucleic acid (RNA) vaccines are mainly used as SARS-CoV-2 vaccines. Despite several issues concerning storage, stability, effective period, and side effects, viral vector vaccines are widely used for the prevention and treatment of various diseases. Recently, viral vector-encapsulated extracellular vesicles (EVs) have been suggested as useful tools, owing to their safety and ability to escape from neutral antibodies. Herein, we summarize the possible cellular mechanisms underlying EV-based SARS-CoV-2 vaccines.
COVID-19 Vaccine Booster Dose Acceptance among Older Adults
Authors: Sezerol Mehmet Akif , Davun Selin
Published: February 2023

This study aimed to determine the factors that cause individuals between the ages of 65 and 75 to not receive the third dose of a COVID-19 vaccination, to advise those who are hesitant, and to learn their thoughts about taking the third dose. (1) Material and Methods: This cross-sectional study was conducted between April and May 2022 among 2383 older adults aged between 65–75 who had never received a COVID-19 booster vaccination, according to the records of the District Health Directorate in the Sultanbeyli district of Istanbul. A three-part questionnaire prepared by researchers was given to the older adults via telephone. For statistical analysis of the data, the Chi-square test was used to compare variables; p < 0.05 was considered statistically significant. (2) Results: This research was completed with 1075 participants, reaching 45% of people aged 65–75 who did not receive the third dose of the COVID-19 vaccine in the region. In total, 64.2% of the participants were female and 35.8% were male, and the mean age was 69.33 ± 2.88. Those who had previously received an influenza vaccine were 1.9 times (95% CI 1.22–2.99) more likely to seek vaccination. Educational status also played a role, as older adults who were uneducated were 0.5 times (95% CI 0.42–0.76) less likely to seek vaccination. In addition, those who stated that lack of time was the reason for not vaccinating were 1.4 times (95% CI 1.01–1.98), and those who did not have it due to forgetting, 5.6 times (95% CI 2.58–12.24), more likely to seek vaccination. (3) Conclusion: This study shows, in detail, the importance of informing older adults, who have not received the third dose of vaccine for COVID-19 and who are in the risk group, as well as those who are not fully vaccinated, about the risks of not being vaccinated. We believe that it is important to vaccinate older patients; further, since immunity conferred by vaccination may decline over time, mortality rates decrease with the administration of additional doses.
Significance of Conserved Regions in Coronavirus Spike Protein for Developing a Novel Vaccine against SARS-CoV-2 Infection
Authors: Olukitibi Titus A. , Ao Zhujun , Warner Bryce , Unat Rodrigo , Kobasa Darwyn , Yao Xiaojian
Published: February 2023

Over the years, several distinct pathogenic coronaviruses have emerged, including the pandemic SARS-CoV-2, which is difficult to curtail despite the availability of licensed vaccines. The difficulty in managing SARS-CoV-2 is linked to changes in the variants’ proteins, especially in the spike protein (SP) used for viral entry. These mutations, especially in the SP, enable the virus to evade immune responses induced by natural infection or vaccination. However, some parts of the SP in the S1 subunit and the S2 subunit are considered conserved among coronaviruses. In this review, we will discuss the epitopes in the SARS-CoV-2 S1 and S2 subunit proteins that have been demonstrated by various studies to be conserved among coronaviruses and may be immunogenic for the development of a vaccine. Considering the higher conservancy of the S2, we will further discuss the likely challenges that could limit the S2 subunit from inducing robust immune responses and the promising approaches to increase its immunogenicity.
Vaccinated Yet Booster-Hesitant: Perspectives from Boosted, Non-Boosted, and Unvaccinated Individuals
Authors: Lin Cheryl , Bier Brooke , Tu Rungting , Paat John J. , Tu Pikuei
Published: February 2023

Though available for all age groups in the US, only about half of those vaccinated have obtained a COVID-19 booster. Similar to the unvaccinated, those vaccinated-but-not-boosted may reduce the effectiveness of widespread viral protection. Booster hesitancy differs from general vaccine hesitancy yet remains less researched. We examined booster perceptions across vaccination status using qualitative methodologies. Four focus groups and 11 individual interviews (total n = 32) revealed nuanced changes and differences compared to the first-dose decision. Booster hesitancy stemmed from questions and surprises. Most vaccinated participants accepted the booster, though to varying degrees: enthusiastically with feelings of appreciation and added confidence, passively as an intuitive next step, indifferently following recommendation—“primed” by the yearly flu shot, and reluctantly with worries. The vaccinated-but-not-boosted group expressed confusion about the need for a new shot and discontentment as to why it was not communicated from the start, which coincided with their uncertainty about ending the pandemic. Inadvertently, booster recommendations further polarized non-vaccinated participants, augmenting their skepticism of the original dosages’ efficacy or necessity and intensifying their distrust of the government. The findings illuminate the need for adjusting vaccination promotions to better tailor communications (e.g., distinguishing its benefits from the first vaccine and emphasizing the continued risk of COVID-19 spread). Future researchers should further explore the vaccine-accepting-yet-booster-hesitant groups’ motivations and risk perceptions to reduce booster rejection.
Therapeutic Potential of Luteolin on Cancer
Authors: Çetinkaya Melisa , Baran Yusuf
Published: February 2023

Cancer is a global concern, as the rate of incidence is increasing each year. The challenges related to the current chemotherapy drugs, such as the concerns related to toxicity, turn to cancer therapeutic research to discover alternative therapy strategies that are less toxic to normal cells. Among those studies, the use of flavonoids—natural compounds produced by plants as secondary metabolites for cancer therapy—has been a hot topic in cancer treatment. Luteolin, a flavonoid that has been present in many fruits, vegetables, and herbs, has been identified to exhibit numerous biological activities, including anti-inflammatory, antidiabetic, and anticancer properties. The anticancer property of Luteolin has been extensively researched in many cancer types and has been related to its ability to inhibit tumor growth by targeting cellular processes such as apoptosis, angiogenesis, migration, and cell cycle progression. It achieves this by interacting with various signaling pathways and proteins. In the current review, the molecular targets of Luteolin as it exerts its anticancer properties, the combination therapy that includes Luteolin with other flavonoids or chemotherapeutic drugs, and the nanodelivery strategies for Luteolin are described for several cancer types.
Does the Condition of the Road Markings Have a Direct Impact on the Performance of Machine Vision during the Day on Dry Roads?
Authors: El Krine Abdessamad , Redondin Maxime , Girard Joffrey , Heinkele Christophe , Stresser Aude , Muzet Valérie
Published: February 2023

The forthcoming arrival of automated vehicles (AV) on the roads requires the re-evaluation or even adaptation of existing infrastructures as they are currently designed on the basis of human perception. Indeed, advanced driver-assistance systems (ADAS) do not necessarily have the same needs as drivers to detect road markings. One of the main challenges related to AV is the optimisation of the vehicle–infrastructure pair in order to guarantee the safety of all users. In this context, we compared the performance of a vehicle equipped with an ADAS machine-vision system with a dynamic retroreflectometer during the daytime on a road section. Our results questioned the reliability of the literature thresholds of the luminance contrast ratio on a dry road under sunny conditions. Despite the presence of old and worn road markings, the ADAS camera was able to detect the edge lines in more than 90% of the cases. The non-detections were not related to the poor condition of the markings but to the environmental conditions or the complexity of the infrastructure.
Enhanced Derivation of Customer-Specific Drive System Design Parameters with Time Frame-Based Maximum Load Analysis
Authors: Mieth Raphael , Markschies Fabian , Zhou Ruixin , Gauterin Frank , Stephan Arnd
Published: February 2023

Only a small part of the high performance of electric drive systems in vehicles is used in everyday operation by customers. As a result, most drives are not operated in the optimum efficiency range. Designing a suitable drive system, whose performance is aligned with actual customer requirements, presents the potential to increase efficiency. Based on the findings of previous research, this paper serves to complement an existing method, which already introduced the basic method of transferring statistical customer data into relevant parameters for the design of a customer-specific drive system. In order to improve the method, further criteria for the selection of relevant time series come into place. Furthermore, the impact on maximum loads resulting from various sequences of the selected time series is identified and evaluated with time frame-based analysis. A new approach for the effective computation of maximum design-relevant loads in the admissible time frame range is introduced and validated. By taking this approach, the sensitivity of the derived design parameters regarding various time series sequence is evaluated in the context of selected datasets. In addition, concatenations of time series are identified which may have a relevant influence on the maximum loads. Consequently, the design process is safeguarded thoroughly against potential maximum loads as well as the associated thermal stresses.
Histological Findings and T2 Relaxation Time in Canine Menisci of Elderly Dogs—An Ex Vivo Study in Stifle Joints
Authors: Bunzendahl Lena , Moussavi Amir , Bleyer Martina , Dehnert Jana , Boretius Susann , Neumann Stephan
Published: February 2023

Osteoarthritis is a chronic disease that often affects the canine stifle joint. Due to their biomechanical function, the menisci in the canine stifle play an important role in osteoarthritis. They compensate for the incongruence in the joint and distribute and minimize compressive loads, protecting the hyaline articular cartilage from damage. Meniscal degeneration favors the development and progression of stifle joint osteoarthritis. Qualitative magnetic resonance imaging (MRI) is the current golden standard for detecting meniscal changes, but it has limitations in detecting early signs of meniscal degeneration. A quantitative MRI offers new options for detecting early structural changes. T2 mapping can especially visualize structural changes such as altered collagen structures and water content, as well as deviations in proteoglycan content. This study evaluated T2 mapping and performed a histological scoring of menisci in elderly dogs that had no or only low radiographic osteoarthritis grades. A total of 16 stifles from 8 older dogs of different sex and breed underwent ex vivo magnet resonance imaging, including a T2 mapping pulse sequence with multiple echoes. A histological analysis of corresponding menisci was performed using a modified scoring system. The mean T2 relaxation time was 18.2 ms and the mean histological score was 4.25. Descriptive statistics did not reveal a correlation between T2 relaxation time and histological score. Ex vivo T2 mapping of canine menisci did not demonstrate histological changes, suggesting that early meniscal degeneration can be present in the absence of radiological signs of osteoarthritis, including no significant changes in T2 relaxation time.
Flood or Non-Flooded: A Comparative Study of State-of-the-Art Models for Flood Image Classification Using the FloodNet Dataset with Uncertainty Offset Analysis
Authors: Jackson Jehoiada , Yussif Sophyani Banaamwini , Patamia Rutherford Agbeshi , Sarpong Kwabena , Qin Zhiguang
Published: February 2023

Natural disasters, such as floods, can cause significant damage to both the environment and human life. Rapid and accurate identification of affected areas is crucial for effective disaster response and recovery efforts. In this paper, we aimed to evaluate the performance of state-of-the-art (SOTA) computer vision models for flood image classification, by utilizing a semi-supervised learning approach on a dataset named FloodNet. To achieve this, we trained son 11 state-of-the-art (SOTA) models and modified them to suit the classification task at hand. Furthermore, we also introduced a technique of varying the uncertainty offset λ in the models to analyze its impact on the performance. The models were evaluated using standard classification metrics such as Loss, Accuracy, F1 Score, Precision, Recall, and ROC-AUC. The results of this study provide a quantitative comparison of the performance of different CNN architectures for flood image classification, as well as the impact of different uncertainty offset λ. These findings can aid in the development of more accurate and efficient disaster response and recovery systems, which could help in minimizing the impact of natural disasters.
Comprehensive Methodology and Analysis to Determine the Environmental Flow Regime in the Temporary Stream “La Yerbabuena” in Aguascalientes, Mexico
Authors: Reyes-Cedeño Isaí Gerardo , Hernández-Marín Martín , Pacheco-Guerrero Anuard Isaac , Gannon John P.
Published: February 2023

In this study, a comprehensive methodology was adapted to determine the environmental flow regime of “La Yerbabuena”, a temporary stream located in the Aguascalientes Valley, Mexico. The analysis was divided into four stages: the geomorphological watershed analysis, a hydrologic analysis, hydraulic modeling, and environmental analysis. The main geomorphological features of the study area were defined from maps in the spatial block, and with them, a synthetic series of daily and monthly discharge was determined and further used in the next stages. In the hydrological stage, the IHA (Indicators of Hydrologic Alteration) methodology and the procedures from the Mexican regulation, named NMX-159, were applied to the stream, and their results were comparatively analyzed. A similar interannual flow variation from both methodologies was found for wet and dry seasons, ranging from 0.010 to 0.108 m3/s. In the hydraulic modeling stage, a micro-basin part of the stream was modeled in the software HEC RAS, observing that the IHA methodology results had water levels that matched the baseflow of the stream, which allows understanding the hydraulic behavior of the water flow through the generation of different profiles in function of the rainy season. Finally, for the environmental stage, the hydrological health of the stream was evaluated using the software Flow Health, additionally observing that the IHA methodology was closer to the desired water level of the reference. This study demonstrates that the proposed methodology achieves the objectives defined by the NMX-159, which establishes a streamflow regime considering a natural interval of hydrologic variability in both ordinary and after-disturbance conditions. This application of the methodology for temporary streams provides an understanding of the hydrological behavior of the environmental flow throughout the year, and regarding the existing regulations, it presents a correlation with the obtained results, as well as greater precision in the dry season.
Combined Effects of Cadmium and Azithromycin on Soil Nitrification Process
Authors: Beduk Fatma
Published: February 2023

Heavy metals and pharmaceuticals have polluted agricultural soils mainly through wastewater irrigation, fertilizers, and soil amendment with sewage sludge. This study aims to determine the synergetic toxic effect of Cd and the selected macrolide antibiotic, azithromycin (AZI), on ammonia-oxidizing bacteria (AOB) in soil, via analyzing nitrification inhibition. A short-term acute toxicity test was used to measure the formation of Nitrite (NO2-N) to indicate the nitrification potential of the aerobic nitrosomonas bacteria in the germination period. Potential nitrification rates (PNRs) of five soil samples ranged between 3.782–17.642 mg NO2-N/kg dm PNRs of soil samples positively correlated with organic matter content and neutral pH. PNRs of the tested soils were significantly affected by Cd and AZI contamination, with interactions exhibited for their simultaneous occurrence and soil pH. A significant difference (p < 0.05) was found when soil samples with pH 6.5–pH 8.5 contaminated with environmentally relevant concentrations of Cd (1 mg/kg–21 mg/kg) and AZI (1 mg/kg–9 mg/kg). 50% PNR inhibition after 11 mg/kg Cd and 5 mg/kg AZI contamination was determined for the soil sample at pH 8.5, with 3.782 mg NO2-N/kg dm potential. From these outcomes, it was concluded that there was a risk of the soil nitrification process in case of simultaneous occurrence of Cd and AZI.