bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comparison of Working Conditions and Workers’ Perceptions among On-Site, Telework, and Hybrid Workers in Ecuador during the COVID-19 Pandemic
Authors: Vinueza-Cabezas Andrea , Osejo-Taco Gabriel , Unda-López Alejandro , Paz Clara , Hidalgo-Andrade Paula
Published: November 2022

The COVID-19 pandemic has forced many companies to adopt different work modalities to ensure their operation during this period. In this study, we described and compared working conditions and perceptions among face-to-face workers, teleworkers, and hybrid workers in Ecuador. A cross-sectional study was conducted with a sample of 542 participants, using a self-report survey to assess sociodemographic data, working conditions, and workers’ perceptions. Variables were described and then compared by the Chi-square test, ANOVA, and the Kruskal–Wallis test. The results indicated a higher proportion of on-site workers without higher education and in the public sector compared to the other modalities. At the same time, there was evidence of increased perceived productivity. People in the hybrid modality tended to have more than one job, earning a higher monthly salary, perceiving a decrease in productivity, an increase in daily working hours, and a lower capacity for time management. In addition, most teleworkers reported fair working conditions, a dedicated workspace, and easy adaptation to this work mode. This study builds a more in-depth understanding of how workers perceived their working conditions among work modalities for organizational decision-making because the evolution of the COVID-19 pandemic is modifying the ways of working permanently.
Influence of Atmospheric Phosphorus and Nitrogen Sedimentation on Water Quality in the Middle Route Project of the South-to-North Water Diversion in Henan Province
Authors: Qiu Yunlin , Zhang Yun , Lan Pengcheng , Liu Han , Wang Hongtian , Wang Wanping , Zhao Peng , Li Yuying
Published: November 2022

In order to understand the potential effects of atmospheric nitrogen and phosphorus deposition on the water quality of the Middle Route Project of the South-to-North Water Diversion Project, samples of dry and wet deposition of atmospheric nitrogen and phosphorus, meteorological factors, and water quality factors were analyzed out to investigate in the Middle Route Project of the South-to-North Water Diversion in Henan Province from October 2018 to October 2020. The variation characteristics of atmospheric nitrogen and phosphorus deposition with time in the Henan section of the main canal are revealed, and the influence of atmospheric dry and wet deposition on the water quality of the middle line is discussed. It was found that the total nitrogen (TN) sedimentation flux has obvious seasonal variation, which was consistent with the variation trend of rainfall, and increased with the increase of rainfall. Nitrogen and phosphorus deposition was significantly correlated with water factors. The effects of meteorological factors and nitrogen and phosphorus deposition on water quality variation reached 18%. The contribution rate and ecological impact of atmospheric nitrogen and phosphorus deposition on water pollution of main channels will be increasing, which needs to be paid enough attention to.
The Impact of Information Infrastructure on Air Pollution: Empirical Evidence from China
Authors: Zhang Pei , Chen Peiran , Xiao Fan , Sun Yong , Ma Shuyan , Zhao Ziwei
Published: November 2022

Information infrastructure construction has become an essential support for the new global technological revolution and industrial change. To examine whether information infrastructure can mitigate the level of air pollution, this paper measures the development level of information infrastructure in each region using the entropy-TOPSIS method based on the data of 31 Chinese provinces from 2013 to 2020. On this basis, it explores the impact of information infrastructure on atmospheric pollution and its mechanism using spatial measures and mediating effects. The results show that: (1) Information infrastructure can effectively improve air quality, though its spatial spillover effect is not obvious. (2) In addition to directly reducing air pollution, information infrastructure can also improve air quality by influencing industrial structure upgrading, or by influencing technological innovation first and then industrial structure upgrading. By exploring the impact of information infrastructure on air pollution and its action path, this paper expects to provide some scientific reference value for the construction of information infrastructure under the background of the new global technological revolution.
Access of People Living with HIV to Testing Services in Greece: A Challenge for Equity in Care
Authors: Souliotis Kyriakos , Tsiakalakis George , Golna Christina , Sifaki-Pistolla Dimitra , Dedes Nikos , Lazanas Marios
Published: November 2022

(1) Background: Access to laboratory testing services for HIV in Greece is persistently challenged and this impacts both the continuum of care and, potentially, equity in access. (2) Methods: A cross-sectional study with two parts (first part: HIV-positive people/PLWHIV; second part: HIV clinicians) was conducted in Greece to quantify challenges regarding access to laboratory testing for HIV. Data were collected through online surveys, during a one-month period, between 2019 and 2020. The total sample consisted of 153 PLWHIV and 26 HIV clinicians. (3) Results: Access to viral load testing varied significantly according to place of residence (p = 0.029) and year of diagnosis (p = 0.054). Patients diagnosed after 2015 reported worse access to viral load testing (72.7% vs. 85.9%). Over one third of respondents perceived viral load tests as being not at all accessible (11.4%) or somewhat accessible, only after facing multiple systemic obstacles (24.2%). Equally, most of HIV clinicians reported barriers or no access to baseline viral load testing (80%) and baseline genotype resistance tests (96%). (4) Conclusions: Access of people diagnosed with HIV to CD4 lymphocyte tests and genotype resistance screening is significantly challenged in Greece, especially after 2015. Addressing this challenge is critical in removing access barriers and achieving the UNAIDS 95-95-95 HIV elimination goals.
Judicial Response to Ecological Environment Risk in China—From the Perspective of Social Systems Theory
Authors: Sang Tian , Liu Peng , Zhao Liang
Published: November 2022

In modern society, law is one of the most important means of risk prevention and control. Under the challenge of ecological and environmental risks, China’s legal governance experience provides important historical experience and theoretical samples for other countries. Faced with problems, such as the difficulty of eliminating risks, risk decisions themselves bring risks, and the huge social cost of risk response, the social system theory can provide novel and new ideas for the cognition and response of environmental risks. Combining the experience of judicial practice with social theory, especially Niklas Luhmann’s doctrine of the risk/danger dichotomy, a clearer functional orientation can be given to judicial powers based on risk communication and risk attribution. By reviewing the ecological judicial practices in China, Germany, and other countries, the role of the legal system in stabilizing the normative expectations of the whole of society can be summarized, which will provide a reference for the risk response and legal governance of the global ecological environment.
Motor Control and Regularity of Menstrual Cycle in Ankle and Knee Injuries of Female Basketball Players: A Cohort Study
Authors: Vico-Moreno Elena , Sastre-Munar Andreu , Fernández-Domínguez Juan Carlos , Romero-Franco Natalia
Published: November 2022

Inadequate motor control facilitates ankle and knee injuries in female basketball. Although biomechanical analysis could help to detect it, aspects such as irregular menstruation make these associations controversial. We aimed to evaluate associations between 2D biomechanics during landing and proprioception with ankle and knee injuries of female basketball players, considering their menstruation regularity. Seventy-one players participated in this study. In the preseason, participants performed a drop-jump to obtain biomechanics during landing and a weight-bearing proprioception test. During the competitive season, all the non-contact ankle and knee injuries were registered. Data showed that 16% of players sustained an ankle or knee injury, being more frequent in players with irregular menstruation compared to regulars (22% vs. 13%, χ2 = 6.009, p = 0.050, d = 0.6). Players who sustained a left-side injury displayed higher left-side dynamic valgus during landing than uninjured players (χ2 = 25.88, p = 0.006, d = 1.5). The rest of the variables did not show any significant difference (p > 0.05). Monitoring 2D dynamic valgus from a drop-jump could help to detect inadequate motor control that may facilitate ankle or knee injuries of female basketball players, mainly for those with irregular menstruation. Proprioception seems not to be related to injuries.
The Mental Health Impact of the COVID-19 Pandemic on Older Adults in China: A Systematic Review
Authors: Liu Jingyuan , Kwan Crystal , Deng Jie , Hu Yuxi
Published: November 2022

Considered at a high risk during the COVID-19 pandemic, older adults in China not only face the disadvantages caused by their relatively low immune systems, but also the challenges brought about by the complex psychological environment in which they spend this special period of their life. However, a thorough study on the impact of the pandemic on older adults’ mental health in China remains scant. Hence, this research aimed to investigate the question: What are the mental health outcomes and associated risk factors of the COVID-19 pandemic on older adults in China? Two Chinese academic databases (China National Knowledge Infrastructure and WANFANG DATA) as well as six English academic databases (PubMed, Scopus, Web of Science, MEDLINE, Social Science, and Google Scholar) were searched while following PRISMA guidelines. Studies were selected according to the predetermined inclusion criteria. Further, relatively high detective rates of mental health disorders, including anxiety symptoms (4.9% to 48.6%), depression symptoms (13.8% to 58.7%), hypochondria (11.9%), suicidal ideation (4.1%), along with worries and fear (55.7%) were all reported. The COVID-19 pandemic has presented a threat to not only the physical, but also the psychological health of Chinese older adults. The most common risk factors of psychological distress among Chinese older adults were found in female gender, living in rural areas, coexisting chronic diseases, and insufficient knowledge about the COVID pandemic. As a result, government policy and psychological guidelines that are created in order to alleviate the adverse effects of COVID-19 on older adults’ mental health, need to be further developed.
Evaluating the COVID-19 Containment Protocol in Greek Universities for the Academic Year 2021–2022
Authors: Rachaniotis Nikolaos P.
Published: November 2022

The COVID-19 pandemic severely disrupted European universities’ educational process. With the vaccination rollout, in-class instruction broadly resumed beginning in September 2021. In order to mitigate the risks of SARS-CoV-2 transmission, European universities apply COVID-19 containment protocols. The aim of this paper is to evaluate the COVID-19 containment protocol that Greek universities implemented in order to fully reopen in the fall of 2021 and for the entire academic year 2021–2022. A case study was conducted at the Department of Industrial Management and Technology, University of Piraeus (Athens’ port), Greece. Data were collected from November 2021 to July 2022 and a quantitative statistical analysis (descriptive and inferential) was performed. A total of 330 unique (and 43 reinfections) COVID-19 cases were confirmed, including 241 undergraduate students, 73 postgraduate, and 2 doctoral students, 10 faculty, and 4 administrative personnel. Contact tracing reported four confirmed and eight potential cases of in-classroom transmission. The person in charge of implementing the COVID-19 containment protocol in the department ordered more than 6000 rapid tests during this period. The Department of Industrial Management and Technology at the University of Piraeus used a rigorously monitored and coordinated strategy of vaccine promotion, screening/testing, contact tracing, isolation, and quarantine in order to control COVID-19 transmission. The results show, on one hand, that the protocol’s implementation is effective and leads to in-classroom transmission minimization and, on the other hand, verify the hypothesis that the department’s confirmed COVID-19 cases are less (with a mean percentage difference of 50%) than the community’s respective 18–39 age group.
How Do Different Urban Footpath Environments Affect the Jogging Preferences of Residents of Different Genders? Empirical Research Based on Trajectory Data
Authors: Zhong Qikang , Li Bo , Chen Yue
Published: November 2022

In recent years, the impact of the urban environment on residents’ physical activity (PA) has received extensive attention, but whether this impact has differences in the jogging preferences of residents in different footpath environments and different genders requires further research. Therefore, based on jogging trajectory data, this paper uses the grouping multiple linear regression model to study the different influencing factors of different footpath environments on the jogging of residents of different genders. The results show that (1) jogging activities (JA) were mainly concentrated in the community footpath environment, and its peak was reached at night; (2) the rise and fall of elements in built environments, social environments, and natural environments significantly affected the relative jogging distance of residents; (3) Residential land density (RLD) has a positive impact on the JA of community and green land footpaths and has a negative impact on the JA of urban footpaths. However, arterial road density (ARD) and bus distance density (BDD) have opposite significant effects on the JA of communities and green land footpaths; (4) ARD has the significant opposite effect on the JA for residents of different genders on urban footpaths and community footpaths. Facilities diversity (FD), population density (PD), and bus stop density (BSD) also had significant opposite effects on the JA of residents of different genders on green land footpaths. In general, we put forward a method theory to identify the footpath environment and provide references for improving the layout and construction of different gender residents for different footpath environment elements.
Women’s Autonomy and Intimate Partner Violence in Peru: Analysis of a National Health Survey
Authors: Barón-Lozada Francisco , Basualdo-Meléndez Gianfranco , Vargas-Fernández Rodrigo , Hernández-Vásquez Akram , Bendezu-Quispe Guido
Published: November 2022

To assess the association between women’s autonomy and intimate partner violence (IPV) against women of childbearing age. Secondary analysis of the 2019 Demographic and Family Health Survey (ENDES-acronym in Spanish) was carried out. The study population was women aged 15–49 years who are currently married or living with a partner. A Poisson family generalized linear regression model was estimated to calculate adjusted prevalence ratios (aPR) for the association between women’s autonomy and IPV with their respective 95% confidence intervals (CI). Data from 18,621 women were analyzed. The highest proportion of women had low autonomy (low: 42%; moderate: 39.2%; high: 18.8%). A prevalence of IPV of 40.1% was found (psychological/verbal: 38.8%; physical: 8.8%; sexual: 2.3%). The adjusted model found that women with a low level of autonomy (aPR: 1.15, 95%CI: 1.01–1.31) had a higher prevalence of IPV compared to women with high autonomy. This association was also found for the specific case of psychological/verbal violence (aPR: 1.15, 95%CI: 1.01–1.31). No association was found between women’s level of autonomy and physical or sexual violence by a partner. Four out of 10 women of childbearing age have experienced IPV in the last 12 months. In general, women with lower levels of autonomy are more likely to present IPV compared to women with high autonomy.
Physical Activity and Social Support to Promote a Health-Promoting Lifestyle in Older Adults: An Intervention Study
Authors: Huang Wei-Yang , Huang Hsuan , Wu Cheng-En
Published: November 2022

The purpose of this study was to understand the effect of intervention in the form of physical activity and social support while co-exercising to promote a health-promoting lifestyle in older adults. This study openly recruited 60 healthy male older adults, all of whom wore a wrist-worn Garmin device walker. Experimental group A was monitored by a special person and walked together as a group, experimental group B walked independently outdoors, and the control group went about their daily routine as normal. All participants recorded daily steps and calorie consumption data for eight weeks. The results of the study showed that when walking together in experimental group A, the number of daily walks and calories consumed could be maintained at a high level due to the effect of group and social support. The number of daily walks in experimental group B was inconsistent due to the lack of motivation to walk on their own. The control group consumed the lowest number of calories in their daily routine. The results show that physical activity and social support have positive explanatory power for a health-promoting lifestyle. Finally, physical activity in older adults should be promoted, and social support for interaction with peers can effectively promote a healthy lifestyle and respond to the arrival of the super-aged society in advance.
The Impact of Backbone Fluorination and Side-Chain Position in Thiophene-Benzothiadiazole-Based Hole-Transport Materials on the Performance and Stability of Perovskite Solar Cells
Authors: Tepliakova Marina M. , Kuznetsov Ilya E. , Mikheeva Aleksandra N. , Sideltsev Maxim E. , Novikov Artyom V. , Furasova Aleksandra D. , Kapaev Roman R. , Piryazev Alexey A. , Kapasharov Artur T. , Pugacheva Tatiana A. , Makarov Sergei V. , Stevenson Keith J. , Akkuratov Alexander V.
Published: November 2022

Perovskite solar cells (PSCs) currently reach high efficiencies, while their insufficient stability remains an obstacle to their technological commercialization. The introduction of hole-transport materials (HTMs) into the device structure is a key approach for enhancing the efficiency and stability of devices. However, currently, the influence of the HTM structure or properties on the characteristics and operational stability of PSCs remains insufficiently studied. Herein, we present four novel push-pull small molecules, H1-4, with alternating thiophene and benzothiadiazole or fluorine-loaded benzothiadiazole units, which contain branched and linear alkyl chains in the different positions of terminal thiophenes to evaluate the impact of HTM structure on PSC performance. It is demonstrated that minor changes in the structure of HTMs significantly influence their behavior in thin films. In particular, H3 organizes into highly ordered lamellar structures in thin films, which proves to be crucial in boosting the efficiency and stability of PSCs. The presented results shed light on the crucial role of the HTM structure and the morphology of films in the performance of PSCs.
AmyP53, a Therapeutic Peptide Candidate for the Treatment of Alzheimer’s and Parkinson’s Disease: Safety, Stability, Pharmacokinetics Parameters and Nose-to Brain Delivery
Authors: Di Scala Coralie , Armstrong Nicholas , Chahinian Henri , Chabrière Eric , Fantini Jacques , Yahi Nouara
Published: November 2022

Neurodegenerative disorders are a major public health issue. Despite decades of research efforts, we are still seeking an efficient cure for these pathologies. The initial paradigm of large aggregates of amyloid proteins (amyloid plaques, Lewis bodies) as the root cause of Alzheimer’s and Parkinson’s diseases has been mostly dismissed. Instead, membrane-bound oligomers forming Ca2+-permeable amyloid pores are now considered appropriate targets for these diseases. Over the last 20 years, our group deciphered the molecular mechanisms of amyloid pore formation, which appeared to involve a common pathway for all amyloid proteins, including Aβ (Alzheimer) and α-synuclein (Parkinson). We then designed a short peptide (AmyP53), which prevents amyloid pore formation by targeting gangliosides, the plasma membrane receptors of amyloid proteins. Herein, we show that aqueous solutions of AmyP53 are remarkably stable upon storage at temperatures up to 45 °C for several months. AmyP53 appeared to be more stable in whole blood than in plasma. Pharmacokinetics studies in rats demonstrated that the peptide can rapidly and safely reach the brain after intranasal administration. The data suggest both the direct transport of AmyP53 via the olfactory bulb (and/or the trigeminal nerve) and an indirect transport via the circulation and the blood–brain barrier. In vitro experiments confirmed that AmyP53 is as active as cargo peptides in crossing the blood–brain barrier, consistent with its amino acid sequence specificities and physicochemical properties. Overall, these data open a route for the use of a nasal spray formulation of AmyP53 for the prevention and/or treatment of Alzheimer’s and Parkinson’s diseases in future clinical trials in humans.
Striatal-Inoculation of α-Synuclein Preformed Fibrils Aggravated the Phenotypes of REM Sleep without Atonia in A53T BAC-SNCA Transgenic Mice
Authors: Okuda Shinya , Nakayama Takeo , Uemura Norihito , Hikawa Rie , Ikuno Masashi , Yamakado Hodaka , Inoue Haruhisa , Tachibana Naoko , Hayashi Yu , Takahashi Ryosuke , Egawa Naohiro
Published: November 2022

Accumulation of α-synuclein (α-syn) is the pathological hallmark of α-synucleinopathy. Rapid eye movement (REM) sleep behavior disorder (RBD) is a pivotal manifestation of α-synucleinopathy including Parkinson’s disease (PD). RBD is clinically confirmed by REM sleep without atonia (RWA) in polysomnography. To accurately characterize RWA preceding RBD and their underlying α-syn pathology, we inoculated α-syn preformed fibrils (PFFs) into the striatum of A53T human α-syn BAC transgenic (A53T BAC-SNCA Tg) mice which exhibit RBD-like phenotypes with RWA. RWA phenotypes were aggravated by PFFs-inoculation in A53T BAC-SNCA Tg mice at 1 month after inoculation, in which prominent α-syn pathology in the pedunculopontine nucleus (PPN) was observed. The intensity of RWA phenotype could be dependent on the severity of the underlying α-syn pathology.
Sustained Activation of CLR/RAMP Receptors by Gel-Forming Agonists
Authors: Chang Chia Lin , Cai Zheqing , Hsu Sheau Yu Teddy
Published: November 2022

Background: Adrenomedullin (ADM), adrenomedullin 2 (ADM2), and CGRP family peptides are important regulators of vascular vasotone and integrity, neurotransmission, and fetoplacental development. These peptides signal through CLR/RAMP1, 2, and 3 receptors, and protect against endothelial dysfunction in disease models. As such, CLR/RAMP receptor agonists are considered important therapeutic candidates for various diseases. Methods and Results: Based on the screening of a series of palmitoylated chimeric ADM/ADM2 analogs, we demonstrated a combination of lipidation and accommodating motifs at the hinge region of select peptides is important for gaining an enhanced receptor-activation activity and improved stimulatory effects on the proliferation and survival of human lymphatic endothelial cells when compared to wild-type peptides. In addition, by serendipity, we found that select palmitoylated analogs self-assemble to form liquid gels, and subcutaneous administration of an analog gel led to the sustained presence of the peptide in the circulation for >2 days. Consistently, subcutaneous injection of the analog gel significantly reduced the blood pressure in SHR rats and increased vasodilation in the hindlimbs of adult rats for days. Conclusions: Together, these data suggest gel-forming adrenomedullin analogs may represent promising candidates for the treatment of various life-threatening endothelial dysfunction-associated diseases such as treatment-resistant hypertension and preeclampsia, which are in urgent need of an effective drug.
MED1 Ablation Promotes Oral Mucosal Wound Healing via JNK Signaling Pathway
Authors: Meng Zhaosong , Li Zhe , Guo Shuling , Wu Danfeng , Wei Ran , Liu Jiacheng , Hu Lizhi , Sui Lei
Published: November 2022

Mediator complex subunit 1 (MED1) is a coactivator of multiple transcription factors and plays a key role in regulating epidermal homeostasis as well as skin wound healing. It is unknown, however, whether it plays a role in healing oral mucosal wounds. In this study, we investigate MED1’s functional effects on oral mucosal wound healing and its underlying mechanism. The epithelial-specific MED1 null (Med1epi−/−) mice were established using the Cre-loxP system with C57/BL6 background. A 3 mm diameter wound was made in the cheek mucosa of the 8-week-old mice. In vivo experiments were conducted using HE staining and immunostaining with Ki67 and uPAR antibodies. The in vitro study used lentiviral transduction, scratch assays, qRT-PCR, and Western blotting to reveal the underlying mechanisms. The results showed that ablation of MED1 accelerated oral mucosal wound healing in 8-week-old mice. As a result of ablation of MED1, Activin A/Follistatin expression was altered, resulting in an activation of the JNK/c-Jun pathway. Similarly, knockdown of MED1 enhanced the proliferation and migration of keratinocytes in vitro, promoting re-epithelialization, which accelerates the healing of oral mucosal wounds. Our study reveals a novel role for MED1 in oral keratinocytes, providing a new molecular therapeutic target for accelerated wound healing.
Giant Triton Snail Charonia tritonis Macrophage-Expressed Gene 1 Protein Ct-Mpeg1: Molecular Identification, Expression Analysis, and Antimicrobial Activity
Authors: Liu Wenguang , Liu Bing , Zhang Gege , Yao Gaoyou , Zhang Yang , Cen Xitong , He Maoxian
Published: November 2022

Macrophage-expressed gene 1 proteins (Mpeg1/Perforin-2 (PRF2)) are a family of pore-forming proteins (PFPs) which can form pores and destroy the cell membrane of invading pathogens. However, little information is available regarding the function of Mpeg1 in the giant triton snail Charonia tritonis. In this study, a homolog of Mpeg1 (Ct-Mpeg1) was identified in C. tritonis. The predicted protein of Ct-Mpeg1 contains several structural features known in Mpegs, including a membrane attack complex/perforin (MACPF) domain and single transmembrane region. The Ct-Mpeg1 gene was constitutively expressed in almost all tissues examined except in the proboscis, with the highest expression level observed in the mantle. As a typical pore-forming protein, Ct-Mpeg1 has antibacterial activities against Vibrio (including Vibrio alginolyticus and Vibrio parahaemolyticus). In addition, rCt-Mpeg1 challenge to V. alginolyticus represses the expression of most outer membrane protein synthesis-related genes and genes involved in the TCA cycle pathway, which will lead to reduced outer membrane protein synthesis and less energy capacity. This is the first report to characterize the macrophage-expressed gene 1 protein in C. tritonis, and these results suggest that macrophage-expressed gene 1 protein Ct-Mpeg1 is an important immune molecule of C. tritonis that is involved in the bacterial infection resistance of Vibrio, and this study may provide crucial basic data for the understanding of the innate immunity system of C. tritonis.
Characterization of BCMA Expression in Circulating Rare Single Cells of Patients with Plasma Cell Neoplasms
Authors: Ndacayisaba Libere J. , Rappard Kate E. , Shishido Stephanie N. , Setayesh Sonia M. , Tang Guilin , Lin Pei , Matsumoto Nicholas , Hsu Ching-Ju , Nevarez Rafael , Velasco Carmen Ruiz , Naghdloo Amin , Yang Eric , Kelly Kevin , Hicks James , Mason Jeremy , Orlowski Robert Z. , Manasanch Elisabet E. , Kuhn Peter
Published: November 2022

B-cell maturation antigen (BCMA), a key regulator of B-cell proliferation and survival, is highly expressed in almost all cases of plasma cell neoplasms and B-lymphoproliferative malignancies. BCMA is a robust biomarker of plasma cells and a therapeutic target with substantial clinical significance. However, the expression of BCMA in circulating tumor cells of patients with hematological malignancies has not been validated for the detection of circulating plasma and B cells. The application of BCMA as a biomarker in single-cell detection and profiling of circulating tumor cells in patients’ blood could enable early disease profiling and therapy response monitoring. Here, we report the development and validation of a slide-based immunofluorescence assay (i.e., CD138, BCMA, CD45, DAPI) for enrichment-free detection, quantification, and morphogenomic characterization of BCMA-expressing cells in patients (N = 9) with plasma cell neoplasms. Varying morphological subtypes of circulating BCMA-expressing cells were detected across the CD138(+/−) and CD45(+/−) compartments, representing candidate clonotypic post-germinal center B cells, plasmablasts, and both normal and malignant plasma cells. Genomic analysis by single-cell sequencing and correlation to clinical FISH cytogenetics provides validation, with data showing that patients across the different neoplastic states carry both normal and altered BCMA-expressing cells. Furthermore, altered cells harbor cytogenetic events detected by clinical FISH. The reported enrichment-free liquid biopsy approach has potential applications as a single-cell methodology for the early detection of BCMA+ B-lymphoid malignancies and in monitoring therapy response for patients undergoing anti-BCMA treatments.
Role of Non-Coding RNAs in Colorectal Cancer: Focus on Long Non-Coding RNAs
Authors: Lulli Matteo , Napoli Cristina , Landini Ida , Mini Enrico , Lapucci Andrea
Published: November 2022

Colorectal cancer is one of the most common causes of cancer-related deaths worldwide. Despite the advances in the knowledge of pathogenetic molecular mechanisms and the implementation of more effective drug treatments in recent years, the overall survival rate of patients remains unsatisfactory. The high death rate is mainly due to metastasis of cancer in about half of the cancer patients and the emergence of drug-resistant populations of cancer cells. Improved understanding of cancer molecular biology has highlighted the role of non-coding RNAs (ncRNAs) in colorectal cancer development and evolution. ncRNAs regulate gene expression through various mechanisms, including epigenetic modifications and interactions of long non-coding RNAs (lncRNAs) with both microRNAs (miRNAs) and proteins, and through the action of lncRNAs as miRNA precursors or pseudogenes. LncRNAs can also be detected in the blood and circulating ncRNAs have become a new source of non-invasive cancer biomarkers for the diagnosis and prognosis of colorectal cancer, as well as for predicting the response to drug therapy. In this review, we focus on the role of lncRNAs in colorectal cancer development, progression, and chemoresistance, and as possible therapeutic targets.
Oxidative Stress Response in Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells
Authors: Waheed Tawakalitu Okikiola , Hahn Olga , Sridharan Kaarthik , Mörke Caroline , Kamp Günter , Peters Kirsten
Published: November 2022

Reactive oxygen species (ROS) can irreversibly damage biological molecules, a process known as oxidative stress. Elevated ROS levels are associated with immune cell activation. Sustained immune system activation can affect many different cells in the environment. One cell type that has been detected in almost all tissues of the body is mesenchymal stem/stromal cells (MSC). MSC possess proliferation and differentiation potential, thus facilitating regeneration processes. However, the regenerative capacity of MSC might be impaired by oxidative stress, and the effects of long-term oxidative stress on MSC functions are sparsely described. The examination of oxidative stress is often performed by exposure to H2O2. Since H2O2 is rapidly degraded, we additionally exposed the cell cultures to glucose oxidase (GOx), resulting in sustained exposure to H2O2. Using these model systems, we have focused on the effects of short- and long-term oxidative stress on viability, migration, differentiation, and signaling. All cellular functions examined were affected by the applied oxidative stress. The differences that occur between pulsed and sustained oxidative stress indicated higher oxidative stress in MSC upon direct H2O2 exposure, whereas the GOx-induced prolonged exposure to H2O2 seems to allow for better cellular adaptation. The mechanisms underlying these different responses are currently unknown.
Digital Educational Escape Room Analysis Using Learning Styles
Authors: Borrás-Gené Oriol , Díez Raquel Montes , Macías-Guillén Almudena
Published: November 2022

Teachers often need to adapt their teaching methodologies in order to overcome possible limitations and ensure that education does not lose quality in the face of different scenarios that may arise in the educational environment, which are not always the most desirable. Techniques such as the Educational Escape Room (ERE) in higher education, are taking a great increase due to its popularity among young people as a leisure activity. This study shows an educational research based on the application of a Digital Educational Escape Room (DEER) to respond to the limitations of hybrid teaching with students divided between the classroom and their homes. Through the analysis of a control group, with a traditional lecture class, and an experimental group with the use of a pretest and a posttest, with the addition of studying the different learning styles of the students in each group, interesting results and conclusions have been obtained that offer a replicability of this technique for other fields and educational modalities.
CryptoNet: Using Auto-Regressive Multi-Layer Artificial Neural Networks to Predict Financial Time Series
Authors: Ranaldi Leonardo , Gerardi Marco , Fallucchi Francesca
Published: November 2022

When analyzing a financial asset, it is essential to study the trend of its time series. It is also necessary to examine its evolution and activity over time to statistically analyze its possible future behavior. Both retail and institutional investors base their trading strategies on these analyses. One of the most used techniques to study financial time series is to analyze its dynamic structure using auto-regressive models, simple moving average models (SMA), and mixed auto-regressive moving average models (ARMA). These techniques, unfortunately, do not always provide appreciable results both at a statistical level and as the Risk-Reward Ratio (RRR); above all, each system has its pros and cons. In this paper, we present CryptoNet; this system is based on the time series extraction exploiting the vast potential of artificial intelligence (AI) and machine learning (ML). Specifically, we focused on time series trends extraction by developing an artificial neural network, trained and tested on two famous crypto-currencies: Bitcoinand Ether. CryptoNet learning algorithm improved the classic linear regression model up to 31% of MAE (mean absolute error). Results from this work should encourage machine learning techniques in sectors classically reluctant to adopt non-standard approaches.
Discovery of a Lineage of Soil-Dwelling Medetera Species with Multi-Coloured Eyes in Southern Europe (Diptera: Dolichopodidae)
Authors: Pollet Marc , Andrade Rui , Gonçalves Ana , Álvarez Fidalgo Piluca , Camaño Portela José Luis , Belin Frédéric , Mortelmans Jonas , Stark Andreas
Published: November 2022

Seven species in the genus Medetera (Diptera: Dolichopodidae) are described here: Medetera aglaops sp. nov., Medetera corsicana sp. nov., Medetera gibbosipyga sp. nov., Medetera hispanica sp. nov., Medetera lusitana sp. nov., Medetera parva sp. nov., and Medetera rectipyga sp. nov. They all originate from the wider Mediterranean region in southwestern Europe (Iberian Peninsula, southern France, Corsica, Sardinia). The most striking feature in most of the species is the bi- or multicolour pattern of the eyes, most conspicuous in Medetera aglaops sp. nov. and Medetera lusitana sp. nov. This character is shared by the male and female sex and is thus not regarded as Male Secondary Sexual Character. All species belong to the Medetera apicalis species group sensu Bickel and are closely related based on shared characters in the hypandrium and cercus. Interestingly, three species with and four species without the basal pair of anterodorsal and posterodorsal bristles on the mid tibia are represented. This suggests that this diagnostic character has less phylogenetic relevance than previously anticipated. Unlike most Palaearctic Medetera, at least five of the new species are mostly found on rocky substrates in dry biotopes with a sclerophyllous vegetation. This further supports their relationship with species of the Medetera muralis subclade sensu Pollet, Germann and Bernasconi.
Meditation and Irritable Bowel Syndrome, a Systematic Review and Meta-Analysis
Authors: Baboș Cristian-Ioan , Leucuța Daniel-Corneliu , Dumitrașcu Dan Lucian
Published: November 2022

Mind-body interventions have shown efficacy in many conditions that have psychosomatic mechanisms, as well as for other pathologies. The aim of this study was to assess the effectiveness of meditation/mindfulness at improving the symptoms severity, quality of life and other associated mood and mental conditions, measured in patients with irritable bowel syndrome (IBS). A systematic review of randomized controlled trials in adult participants with IBS was conducted. Eight databases were searched for articles. We performed a meta-analysis evaluating the effects of meditation-based therapy on symptomatology, quality of life, anxiety and depression. Out of 604 articles screened, six were selected for quantitative review. The standardized mean difference (SMD) of the mindfulness group and the control group was of −36.95 (95% CI −74.61–0.7), p = 0.054 regarding the IBS symptom score; of 12.58 (95% CI 4.42–20.74), p = 0.003 regarding the IBS quality of life; SMD = 2.8 (95% CI 1.01–4.6), p = 0.002 for spiritual scale; and of 15.49 (95% CI −28.43–−2.55), p = 0.019 regarding the pain score in IBS. Our study found that the quality of life and the spiritual scale scores (i.e., mindful awareness) were statistically significantly higher in the mindfulness group, while the pain score was statistically significantly lower in the mindfulness group.
Does the COVID-19 Pandemic Affect Labor-Related Anxiety and Prevalence of Depressive Symptoms in Pregnant Women?
Authors: Wikarek Agnieszka , Niemiec Agnieszka , Szymanek Małgorzata , Klimek Mateusz , Partyka-Lasota Justyna , Dudzik Kamila , Wikarek Tomasz , Nowosielski Krzysztof
Published: November 2022

The COVID-19 pandemic undoubtedly had significant effects on women’s health and the course of pregnancy. The aim of this single-center study was to explore the impact of the COVID-19 pandemic on adult pregnant and postpartum women’s mental health, as well as to identify factors associated with depressive symptoms, anxiety and fear of delivery. The 465 women included in this questionnaire-based cohort study were divided into two groups: one (controls) of women who gave birth before (n = 190), and the second who were pregnant and delivered during the pandemic (n = 275). The COVID-19 pandemic affected the severity of self-reported anxiety regarding childbirth (mean scores 2.7 vs. 2.36, p = 0.01). The depression (19.84 ± 13.23) and anxiety (16.71 ± 12.53) scores were higher in pregnant women during the COVID 19 pandemic, compared to women who gave birth before the pandemic (8.21 ± 7.38 and 11.67 ± 9.23, respectively). These findings demonstrate the magnitude of the pandemic’s impact on women’s mental health, and actions to improve the mental health of pregnant women in Poland may be crucial for maternal and fetal well-being.
Human Coronary Plaque Optical Coherence Tomography Image Repairing, Multilayer Segmentation and Impact on Plaque Stress/Strain Calculations
Authors: Huang Mengde , Maehara Akiko , Tang Dalin , Zhu Jian , Wang Liang , Lv Rui , Zhu Yanwen , Zhang Xiaoguo , Matsumura Mitsuaki , Chen Lijuan , Ma Genshan , Mintz Gary S.
Published: November 2022

Coronary vessel layer structure may have a considerable impact on plaque stress/strain calculations. Most current plaque models use single-layer vessel structures due to the lack of available multilayer segmentation techniques. In this paper, an automatic multilayer segmentation and repair method was developed to segment coronary optical coherence tomography (OCT) images to obtain multilayer vessel geometries for biomechanical model construction. Intravascular OCT data were acquired from six patients (one male; mean age: 70.0) using a protocol approved by the local institutional review board with informed consent obtained. A total of 436 OCT slices were selected in this study. Manually segmented data were used as the gold standard for method development and validation. The edge detection method and cubic spline surface fitting were applied to detect and repair the internal elastic membrane (IEM), external elastic membrane (EEM) and adventitia–periadventitia interface (ADV). The mean errors of automatic contours compared to manually segmented contours were 1.40%, 4.34% and 6.97%, respectively. The single-layer mean plaque stress value from lumen was 117.91 kPa, 10.79% lower than that from three-layer models (132.33 kPa). On the adventitia, the single-layer mean plaque stress value was 50.46 kPa, 156.28% higher than that from three-layer models (19.74 kPa). The proposed segmentation technique may have wide applications in vulnerable plaque research.
Distribution and Abundance of the Blue Whale (Balaenoptera musculus indica) off Sri Lanka during the Southwest Monsoon 2018
Authors: Kirumbara Liyanage U. S. P. , Krishantha Jayasinghe R. P. P. , Jens-Otto Krakstad , Kanapathipillai Arulananthan
Published: November 2022

Blue whales Balaenoptera musculus are the most abundant and widely distributed cetacean species in Sri Lankan waters. A vessel-based opportunistic line transect survey focusing on marine mammals was conducted in Sri Lankan waters between 24 June to 12 July 2018, while an ecosystem survey was performed by the R/V Dr. Fridtjof Nansen. The entire transect length was 2090 km within an area of 3895 km2. Overall, 72% (n = 57) of blue whale observations were group assemblages ranging between 1 and 5 individuals out of a total of 79 sightings. The largest aggregations of blue whales were recorded at the intersection of the submarine canyon off the coastline of Mirissa and busy shipping lanes between Dondra Head and Galle, where previous ship strikes were recorded. Overall, the average observed group size was 2.64 (CV, 12.34%; 95% CI, 2.07–3.38), the average group density was 0.0029 km−2 (CV, 35.96%; 95% CI, 0.00145–0.00610), and the total blue whale abundance within the survey area was 513 individuals (CV, 38.02%; 95% CI, 243–1083). These survey results fill vital knowledge gaps regarding the abundance and distribution of blue whales in Sri Lanka, which is essential for the establishment of management and conservation strategies.
Detection of Massive Oil Spills in Sun Glint Optical Imagery through Super-Pixel Segmentation
Authors: Sun Zhen , Sun Shaojie , Zhao Jun , Ai Bin , Yang Qingshu
Published: November 2022

Large volumes of crude oil accidentally released into the sea may cause irreversible adverse impacts on marine and coastal environments. Large swath optical imagery, acquired using platforms such as the moderate-resolution imaging spectroradiometer (MODIS), is frequently used for massive oil spill detection, attributing to its large coverage and short global revisit, providing rich data for oil spill monitoring. The aim of this study was to develop a suitable approach for massive oil spill detection in sun glint optical imagery. Specifically, preprocessing procedures were conducted to mitigate the inhomogeneous light field over the spilled area caused by sun glint, enhance the target boundary contrast, and maintain the internal homogeneity within the target. The image was then segmented into super-pixels based on a simple linear clustering method with similar characteristics of color, brightness, and texture. The neighborhood super-pixels were merged into target objects through the region adjacency graph method based on the Euclidean distance of their colors with an adaptive termination threshold. Oil slicks from the generated bright/dark objects were discriminated through a decision tree with parameters based on spectral and spatial characteristics. The proposed approach was applied to oil spill detection in MODIS images acquired during the Montara oil spill in 2009, with an overall extraction precision of 0.8, recall of 0.838, and F1-score of 0.818. Such an approach is expected to provide timely and accurate oil spill detection for disaster emergency response and ecological impact assessment.
Phylogenomic and Evolutionary Analyses Reveal Diversifications of SET-Domain Proteins in Fungi
Authors: Ding Guoqing , Shang Liqiu , Zhou Wenliang , Lu Siyi , Zhou Zong , Huang Xinyi , Li Juan
Published: November 2022

In recent years, many publications have established histone lysine methylation as a central epigenetic modification in the regulation of chromatin and transcription. The histone lysine methyltransferases contain a conserved SET domain and are widely distributed in various organisms. However, a comprehensive study on the origin and diversification of the SET-domain-containing genes in fungi has not been conducted. In this study, a total of 3816 SET-domain-containing genes, which were identified and characterized using HmmSearch from 229 whole genomes sequenced fungal species, were used to ascertain their evolution and diversification in fungi. Using the CLANS program, all the SET-domain-containing genes were grouped into three main clusters, and each cluster contains several groups. Domain organization analysis showed that genes belonging to the same group have similar sequence structures. In contrast, different groups process domain organizations or locations differently, suggesting the SET-domain-containing genes belonging to different groups may have obtained distinctive regulatory mechanisms during their evolution. These genes that conduct the histone methylations (such as H3K4me, H3K9me, H3K27me, H4K20me, H3K36me) are mainly grouped into Cluster 1 while the other genes grouped into Clusters 2 and 3 are still functionally undetermined. Our results also showed that numerous gene duplication and loss events have happened during the evolution of those fungal SET-domain-containing proteins. Our results provide novel insights into the roles of SET-domain genes in fungal evolution and pave a fundamental path to further understanding the epigenetic basis of gene regulation in fungi.