bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengembangan Kawasan Stasiun Tugu dengan Penekanan Wayfinding di Yogyakarta
Authors: Zahrah Yunisa , Handayani Kusumaningdyah Nurul , Mustaqimah Ummul
Published: October 2021

The proposal aims to bring an accessible, time efficient, and secure design of Tugu Train Station in Yogyakarta City. Yogyakarta happens to be located strategically on the main routes of Java. It is one of the reasons why the city becomes a tourist destination, both domestic and international. Tugu Train Station manages to reach passengers number increase by 10-17% annually. The train station has a good prospect to be developed into a multifunctional train station. The design proposal rises some problems: designing exterior spaces which directs users to the site, interior spaces which makes users navigate easily, and a seamless, time efficient, and clear movement flow from the site to the building. The implementation of wayfinding method is based on post-occupancy evaluation of the existing compound in which confusing building layout creates difficulties in mobility for the passengers. Wayfinding method focuses on spatial organisation, movement flow organisation, and signage placement. This design proposal would expand existing train station buildings to provide more rooms in the anticipation of passenger increase for year to come; as well as to build new public facilities. Furthermore, the implementation of wayfinding method is expected to create an accessible, time efficient, and secure layout which in turn caters various types of passenger.
Evaluasi Desain Bangunan Berdasarkan Sistem Sertifikasi DGNB (Studi Kasus : The Khabele School, Austin)
Authors: Wicaksono Dady , Sugini Sugini
Published: October 2021

The air pollution problem had become a big problem for many cities in the world. In line with the rapid development of these cities, the concept of sustainability also continues to develop with various solutions to overcome existing problems. To solve the air pollution problem, the authors evaluated the selected building object, The Khabele School, to see the extent of the building's contribution to overcoming the air pollution problem.  The evaluation was carried out based on the DGNB (German Sustainable Building Council) certification system, the variables measured were biotope area factor (BAF), indoor air quality, daylight factor, and zero-carbon mobility. The results reveal that indoor air quality and daylight factors could meet the desired standards, while BAF and zero-carbon mobility had not met the standard. Based on theoretical and empirical studies, this paper discussed alternative design strategies, to improve the quality of the building, so that they can contribute more to overcoming air pollution problem.
Preferensi Kenyamanan Pengunjung di Kebun Raya Bogor selama Adaptasi Kebiasaan Baru Berbasis Big Data
Authors: Paramitadevi Yudith Vega , Jannah Nurul , Mindara Gema Parasti
Published: October 2021

Urban communities need urban forests in improving the quality of mental  and physical health during the New Normal. If ecotourism or urban forest is a top priority in implementing the New Normal, managers must determine designs, and make procedures related to the applicable COVID-19 protocol. The research objective was to measure the comfort level of visitors before and during the pandemic using big data, then compile comfort design recommendations according to visitor preferences during the pandemic based on survey methods. Social media Twitter and Google Map Review mentioning the Bogor Botanical Garden were collected a year before and six months during the pandemic. Analysis of text mining is carried out in order to obtain sentiment and initial perceptions about the Bogor Botanical Garden. Initial perceptions of visitors were used to construct the survey questionnaire. Furthermore, quantitative survey methods were used to analyze visitor activity and comfort. Visitor comfort is limited to abiotic factors, namely accessibility, and facilities. Sentiment analysis shows pre-pandemic conditions had 48.26% more positive word frequency than during the pandemic for Twitter, but 23.29% less than during the pandemic for Google Map Review. Twitter are more sensitive to the current situation than Google Map Review. Positive perceptions of comfort include compliance management of KRB under the COVID-19 protocol (11.7%), better management (10.8%), well-maintained facilities and infrastructure (7.2%), and maintained hygiene (7.2%). Comfort design recommendations based on respondents' preferences for comfort (14.4%) include increasing the number of toilets, the number of rest areas, ramps for the elderly, areas and attractions to attract children, parks with new themes, and sustainability elements.
Nilai Kearifan Rumah Hijau (Greenship Homes) Permukiman Pedesaan Lereng Gunung, kasus Desa Kledung, Temanggung
Authors: Rejeki V.G. Sri , Ndruru Romantio Veronika , Bella K. Gita Cynthia , Wafi Zia Amalia
Published: October 2021

The implementation of greenship homes rating tools on houses in residential areas has been carried out in recent times. On the other hand, in mountain settlements have the value of local wisdom considers the environment as the basis of settlement management. The purpose of this study found the value of green buildings mountain settlements, the case of Kledung Village, Wonosobo, using the analysis of greenship homes rating tools. Qualitative research is conducted by physical mapping methods and interviews to homeowner informants. The study used 12 purposive samples, which were defined based on four character locations. The results of the study, based on 4 character location of the case, greenship homes value is relatively similar / its same. There is no difference in value between one case than another, so that analysis is done at once. Overall, the results of the study explained that the greenship homes rating tool can be applied in kledung village. The result is 1) all buildings are larger than the ratio of greenship rating tools, 2) all houses have electricity meters and monitoring; 3) sorting of organic and inorganic waste; 4) pest management; 5) some houses use recicled, reuse, and local materials, 6) sunny utilization and solar entry into homes and cross-circulation wind management in houses. Uniquenesse's finding are 1) there is a minimum large tree in Kledung village, because the village has cold temperatures,  high humidity and little daily sunlight time. In addition, the people in Kledung Village do not regulate clean water, because it is abundant in this village
Tektonika Perpaduan Unsur Jawa Dengan Eropa Pada Pendopo Dalem Kabupaten Rembang
Authors: Afriyanto Deny Setya , Putri Pusparini Dharma , Pradipto Eugenius
Published: October 2021

Dalem Kabupaten Rembang (regent residence), including the Pendopo as part of the Dalem component, was built around 1750. This building has a traditional Javanese concept that combined colonial architectural elements. This study aims to see the philosophical aspects of the saka guru that may influence the tectonic fusion of Javanese and European elements. In order to know the tectonic aspect of the Pendopo Dalem Kabupaten, this research using qualitative methods, while data collecting is conducted through literature study and observation. The findings of this study can be seen from the philosophical aspect in the concept of the “papat kiblat lima pancer“, where is reflected in the main structure of the saka guru (wooden material). Where functions as the main structure of the pendopo. Wooden material have given the aspect of its function which resists lateral forces (wind) to the overall construction. Meanwhile, the cement brick material on saka rawa and saka emper continuing the roof load to its foundation, used as bearing wall system. 
THE EFFECT OF RECRUITMENT AND MOTIVATION TOWARD JOB PERFORMANCE OF MILITARY PERSONNEL SUBMARINE UNIT IN EASTERN FLEET COMMAND OF INDONESIAN NAVY
Authors: Kartiwa Iwa
Published: October 2021

The objective of this research is to obtain information on the effect of recruitment and motivation toward job performance of Military Personnel Submarine Unit in Eastern Fleet Command of Indonesian Navy. This research  using survey method with quantitative approach and path analysis technique. The population of research is 80 military personnel and sample involving 67 personnel as respondents were selected through simple random sampling. This research findings are (1) there is a positively direct effect recruitment on job performance. (2) there is a positively direct effect motivation on job performance (3) there is a positively direct effect recruitment on motivation. Therefore, the job performance of Submarine Unit military personnel can be improved through improvement of recruitment, and motivation.
PENGARUH KEPEMIMPINAN DAN IKLIM ORGANISASI TERHADAP REWARD DI SEKOLAH DASAR NEGERI KECAMATAN KEBON JERUK DAN CENGKARENG
Authors: Masnawati Neneng
Published: October 2021

The purpose of this research is to determine the effect of leadership  and organizational climate on reward school community elemtary school in village of kebon jeruk and cengkareng..The research methodology was survey which were selected by simple random sampling technique. Analysis and interpretation of the data indicate that (1) leadership has a positive direct effect in reward, (2) organizational climate has a positive direct effect in reward, (3) leadership  has a positive direct effect in organizational climate.
ARTIFICIAL INTELLIGENCE IN JURISPRUDENCE: PROSPECTS FOR USING AN INTERDISCIPLINARY COGNITIVE MODEL
Authors: Sokolova A.
Published: November 2021

The development of modern technologies, the creation of artificial intelligence have posed new problems for humanity. Is the methodological potential of legal science sufficient
to comprehend modern phenomena and predict positive and negative consequences for society?
The proposed article attempts to modernize the cognitive model by expanding the scope
of an interdisciplinary approach. This is a new research paradigm - the interdisciplinarity of a new order.
As an illustration, the plot of the “penetration” of artificial intelligence in jurisprudence
is given, the question is raised about the risks of violation of human values and measures to prevent them.
Prehabilitative Resistance Exercise Reduces Neuroinflammation and Improves Mitochondrial Health in Aged Mice with Perioperative Neurocognitive Disorders
Authors: Liu Yan , Chu John Man Tak , Ran You , Zhang Yan , Chang Raymond Chuen Chung , Wong Gordon Tin Chun
Published: November 2021

Background

Perioperative neurocognitive dysfunction remains a significant problem in vulnerable groups such as the elderly. While experimental data regarding its possible pathogenic mechanisms accumulates, therapeutic options for this disorder are limited. In this study, we evaluated the neuroprotective effect of a period of preconditioning resistant training on aged mice following abdominal surgery. Further, we examined the underlying mechanism from the perspective of neuroinflammatory state and synaptic plasticity in the hippocampus.
Methods

18-month-old C57BL/6N mice were trained for 5 weeks using a ladder-climbing protocol with progressively increasing weight loading. Preoperative baseline body parameters, cognitive performance and neuroinflammatory states were assessed and compared between sedentary and trained groups of 9-month-old and 18-month-old mice. To access the neuroprotective effect of resistance training on postoperative aged mice, both sedentary and trained mice were subjected to a laparotomy under 3% sevoflurane anesthesia. Cognitive performance on postoperative day 14, hippocampal neuroinflammation, mitochondrial dysfunction and synaptic plasticity were examined and compared during groups.
Results

18-month-old mice demonstrated increased body weight, higher peripheral and central inflammatory status, reduction in muscle strength and cognitive performance compared with middle-aged 9-month-old mice, which were improved by resistance exercise. In the laparotomy group, prehabilitative resistant exercise improved cognitive performance and synaptic plasticity, reduced inflammatory factors and glial cells activation after surgery. Furthermore, resistance exercise activated hippocampal PGC-1α/BDNF/Akt/GSK-3β signaling and improved mitochondrial biogenesis, as well as ameliorated mitochondrial dynamics in postoperative aged mice.
Conclusions

Resistance exercise reduced risk factors for perioperative neurocognitive disorders such as increased body weight, elevated inflammatory markers, and pre-existing cognitive impairment. Accordantly, the preoperative resistance exercise was found to improve surgery-induced adverse effects including cognitive impairment, synaptic deficit and neuroinflammation, possibly by facilitate mitochondrial health through the PGC1-a/BDNF pathway.
NOL12 Acts as an Oncogenic Biomarker and Predicts the Efficacy of Immune Checkpoint Inhibitors in Hepatocellular Carcinoma
Authors: Huang Jianfeng , Kang Weibiao , Pan Shubo , Yu Changjun , Jie Zhigang , Chen Changyu
Published: November 2021

Background: Hepatocellular carcinoma (HCC) is a common malignancy with a poor prognosis worldwide. However, the pathogenesis of HCC remains poorly understood. Methods: Through data mining and analyses of The Cancer Genome Atlas (TCGA) datasets, the NOL12 expression in HCC was determined and the associations between its expression and patient survival and clinicopathological parameters were evaluated. The pro-tumorigenic roles of NOL12 on HCC in vitro were further verified by loss-of-function assay. The correlation between NOL12 expression and tumor-infiltrating immune cells (TICs) was analyzed by CIBERSORTx method. In addition, the risk signature based on 8 NOL12-related genes was established to accurately evaluate the prognosis of patients with HCC and to further predict the efficacy of immune checkpoint inhibitors (ICIs) in HCCResults: We found that NOL12 was significantly overexpressed in independent HCC datasets from TCGA database. High expression of NOL12 is associated with worse reduced overall survival (OS), high pathological grade, node metastasis and advanced clinical stage in patients with HCC. Moreover, NOL12 knockdown significantly inhibited cell proliferation, migration and invasion. CIBERSORTx analysis revealed that twelve types of TICs are correlated with NOL12 expression. The risk signature based on 8 NOL12-related genes is an independent prognostic factor for patients with HCC. The OS rate of patients in the low-risk score group was better than that in the high-risk score group. In addition, the total tumor mutation burden (TMB) in the high-risk score group increased significantly, and the risk scores could be used as an alternative indicator of ICI response. Conclusions: Our findings indicated that NOL12 might be involved in the progression of HCC and can be used as a potential therapeutic target. Moreover, the NOL12-related risk signature may have predictive relevance with regard to ICI therapy.
Extraction Optimization, Structural Characterization, and Anti-Hepatoma Activity of Polysaccharides from Scutellaria Barbata D. Don
Authors: Su Wenwen , Wu Leilei , Liang Qichao , Lin Xiaoyue , Xu Xiaoyi , Yu Shikai , Lin Yitong , Fu Yang , Zhou Jiadong , Zhang Bo , Li Li , Li Dan , Yin Yongkui , Song Gaochen
Published: November 2021

Background The above experimental results show that SBP-2A isolated and purified from Scutellaria barbata may be a candidate drug for further evaluation in cancer prevention, which provides a clue for further studies on the molecular mechanism of its anticancer activity against human liver cancer cells.Methods The crude polysaccharide of Scutellaria barbata (SBP) was extracted with water and precipitated with alcohol. Optimal extraction conditions were determined by response surface methodology: the solid-liquid ratio was 1:25, the extraction time was 2 h, and the extraction temperature was 90 °C. With these conditions, the average extraction efficiency was 3.85 ± 0.13%. SBP was purified with a DEAE-52 cellulose column and Sephadex G-100 dextran gel column to obtain SBP-1A and SBP-2A fractions. The polysaccharide content, molecular weight, monosaccharide composition and basic structure were preliminarily identified. Then, a MTT assay was used to identify the polysaccharide components with anti-hepatoma effects. The antitumor activity of SBP-2A was evaluated by colony formation tests, morphological observations, apoptosis and cell cycle analyses.Results Structural analysis showed that SBP-1A and SBP-2A were mainly composed of arabinose and galactose, but the molar ratios were different; these were homogeneous acidic polysaccharide components with high purity, and the average molecular weights were 1.15 × 105 Da and 1.4 × 105 Da, respectively. FT-IR spectra showed that SBP-1A and SBP-2A contained uronic acid β-glucan, and the sugar residue of the polysaccharide was mainly pyranose. MTT and colony formation assays showed that SBP-2A significantly inhibited the proliferation of HepG2 cells. The cell distribution at different apoptotic stages was determined by the Hoechst 33258 test and Annexin V-FITC/PI staining. Flow cytometric analysis showed that SBP-2A induced HepG2 cell apoptosis by blocking the G1 phase.Conclusions Two polysaccharides (SBP-1A and SBP-2A) had been isolated from Scutellaria barbata. Preliminary characterization of the SBP-1A and SBP-2A was investigated. The anticancer activities were studied in vitro. SBP-2A significantly inhibited the proliferation of HepG2 cells and induced cells apoptosis by blocking the G1 phase.
LncRNA PAXIP1-AS1 is a Prognostic Biomarker and Correlated with Immune Infiltrates in Ovarian Cancer
Authors: Chen Buze , Lu Xiaoyuan , Zhou Qingmei , Chen Qing , Zhu Siyan , Liu Hui , Li Guilin
Published: November 2021

Background: The long non-coding RNA (LncRNA) PAXIP1 antisense RNA 1 (PAXIP1-AS1) was found to promote proliferation, migration, EMT, and apoptosis of ovarian cancer (OC) cells in OC cell lines, but the relationship between PAXIP1-AS1 expression and clinical characteristics, prognosis, and immune infiltration of OC patients and its regulatory network are unclear. Methods: QRT-PCR, Kruskal-Wallis test, Wilcoxon sign-rank test, logistic regression, Kaplan-Meier method, Cox regression analysis, Gene set enrichment analysis (GSEA), and immuno-infiltration analysis were used to evaluate the relationship between clinical characteristics and PAXIP1-AS1 expression, prognostic factors, and determine the significant involvement of PAXIP1-AS1 in function. Results: Low PAXIP1-AS1 expression in OC was associated with age (P=0.045), histological grade (P=0.011), and lymphatic invasion (P=0.004). Low PAXIP1-AS1 expression predicted a poorer overall survival (OS) (HR: 0.71; 95% CI: 0.55–0.92; P=0.009), progression free interval (PFS) (HR: 1.776; 95% CI: 1.067–2.955; P=0.001) and disease specific survival (DSS) (HR: 0.67; 95% CI: 0.51–0.89; P=0.006). And PAXIP1-AS1 expression (HR: 0.711; 95% CI: 0.542-0.934; P=0.014) was independently correlated with PFS in OC patients. GSEA demonstrated that neutrophil degranulation, signaling by Interleukins, GPCR-ligand binding, G alpha I signaling events, VEGFAVEGFR-2 signaling pathway, naba secreted factors, Class A 1 Rhodopsin-Like Receptors, PI3K-Akt signaling pathway, and Focal Adhesion-PI3K-Akt-mTOR-signaling pathway were differentially enriched in PAXIP1-AS1 high expression phenotype. PAXIP1-AS1 may inhibit the function of aDC, B cells, CD8 T cells, Cytotoxic cells, DC, iDC, Macrophages, Mast cells, Neutrophils, NK CD56dim cells, T cells, TFH, Tgd, Th1 cells, Th2 cells and Treg. Conclusions: Low expression of PAXIP1-AS1 was significantly associated with poor survival and immune infiltration in OC. PAXIP1-AS1 could be a promising prognosis biomarker for OC.
Engaging Millennial Students through Flipped Classroom: Perspectives from Faculty, Undergraduate Medical and Nursing Students
Authors: Sultan Amber Shamim , Ali Rahila , Zahid Nida , Akbar Rozmeen , Ali Mehdia Nadeem Rajab , Fatima Sadia , Ghias Kulsoom , Martins Russell , Tariq Muhammad , Ajani Khairulnissa
Published: November 2021

Background: The ‘flipped classroom’ is a teaching pedagogy where students are actively involved in the learning process. It reduces passivity, enables students to become active learners through reasoning and concept application, and facilitates student interaction with their peers and instructors. This instructional approach enhances retention and decreases distraction by engaging students.The purpose of this study was to train the faculty of the medical college and school of nursing in adapting flipped classrooms (FCR) as a strategy and to facilitate them in conducting sessions for their learners. Methods: This study was conducted from July 2019 to September 2020. Three training workshops were conducted for faculty participants. The workshop was designed in a flipped style format in which pre-reading material was shared in advance with the workshop participants. A discussion board was created on Pad-let to engage workshop participants prior to the face-to-face (F2F) session. Kahoot was used to assess prior knowledge at the beginning of the F2F session. A video on flipped classroom was uploaded along with a few questions in between to check their basic understanding of the topic. Workshops’ participants conducted sessions for medical and nursing students and participated in focus group discussions (FGD) to share their experiences. Students’ perspectives were also obtained after their FCR session through a structured questionnaire.Results: Both medical and nursing students found FCR format stimulating. A significantly higher proportion of medical students (73%) found the FCR more engaging and interesting than a traditional lecture as compared to nursing students (59%) (p= 0.009). Similarly, 73 % of medical students believed the learning objectives of both the non-face-to-face (NF2F) and face-to-face (F2F) sessions were shared with them as compared to the 62% of nursing students who believed the same (p=0.002). A significantly higher proportion of medical (76%) versus nursing (61%) students found the FCR format more useful for application of their theoretical knowledge into clinical practice (p=0.030).Conclusion: Students found the flipped classroom (FCR) more engaging and interesting in terms of applying theoretical knowledge into practice. It is recommended to conduct more FCR sessions for an interactive and student-centered learning experience.
Association between Serum Uric Acid to HDL-cholesterol Ratio and Nonalcoholic Fatty Liver Disease among Chinese Adults
Authors: Zhao Hui , Qiu Xia , Cui Jiajia , Sun Yongye
Published: November 2021

We conducted this case-control study to explore the association of serum uric acid (SUA) to HDL-cholesterol (HDL-C) ratio (UHR) with the risk of nonalcoholic fatty liver disease (NAFLD) in general Chinese adults. A total of 636 patients with NAFLD and 754 controls from affiliated hospital of Qingdao University in China between January to December 2016 were involved. NAFLD was diagnosed by ultrasonography after excluding other etiologies. The multivariable adjusted odds ratio and 95% confidence interval (CI) of NAFLD for the highest versus lowest quartile of UHR was 3.888 (2.324-6.504). In stratified analyses by sex and age, the positive associations between UHR and the risk of NAFLD were statistically significant in each subgroup. In stratified by BMI, the significant positive association was only found in the individuals with BMI≥23.9 kg/m2. Dose-response analysis indicated a linear positive correlation between UHR and NAFLD risk.
Oblique Lateral Interbody Fusion Combined with Lateral Plate VS. Combined with Posterior Pedicle Screw Fixation in Lumbar Degenerative Diseases
Authors: Li Hai-dong , Zhong Li , Fang Xiang-Qian , Jiang Lei-Sheng
Published: November 2021

Background The oblique lateral interbody fusion (OLIF) is a minimally invasive indirect decompression technique for the treatment of degenerative spinal disease. OLIF with posterior pedicle screws fixation frequently is performed, whereas it requires much more surgery time and blood loss. The purpose of this study was to compare the oblique lateral interbody fusion (OLIF) combined with lateral plate (LP) vs. OLIF combined with posterior pedicle screw (PS) fixation for the treatment of lumbar degenerative diseases.Methods The clinical data of 53 patients with lumbar degenerative diseases who underwent OLIF from January 2020 to September 2020 were retrospectively analyzed，24 in OLIF combined with lateral plate (OLIF+LP) group and 29 in OLIF combined with pedicle screw (OLIF+PS) group. All patients completed a minimum 1-year follow-up. The duration of operation, blood loss, fusion rate and complications were recorded. The visual analog scale (VAS) score, Oswestry Disability Index (ODI), disc height (DH), foraminal height (FH) and cross-sectional area (CSA) were also evaluated.Results The operation time was 75.41±11.53 min in the OLIF+LP group, which shorter than that in OLIF+PS group(127.05±5.62min, P<0.01). Also, the blood loss was significantly less in the OLIF+LP group (39.55±5.32 ml ) than in the OLIF+PS group (89.81±9.62, P<0.01). The VAS and ODI scores both significantly reduced after operation in OLIF+LP group. There was no difference either in VAS or ODI scores by 1 year after surgery between two groups (P>0.01). The DH, FH, and CSA parameters were all improved significantly after operation in both groups, however, there was no significant difference at the any follow-up point between the two groups. The total complication rate was 13.21% (7/53) in this study, and there was no siginificant difference between the two groups. The fusion rate was 91.67% in the OLIF+LP group and 93.10% in the OLIF+PS group (P =0.69).Conclusions OLIF+LP fixation seems to be a valuable surgical option for single-segmental lumbar degenerative disease, it can achieve much better clinical outcomes than OLIF+PS group.
Kherzet Youcef Mine Flooding of 2nd June 1990 Revisited (Northeastern of Algeria) Origine and Hydrogeological Consequences
Authors: Souhila CHEDDAD , Abdelhamid Haouchine
Published: November 2021

This work is part of the hydrogeological study of the Kherzet Youcef deposit. The polymetallic deposit of Kherzet Youcef, known, since the beginning of the 20th century, by the exploitation of Lead and Zinc ore, is located 50 Km southeast of Setif (North-eastern Algeria) and 5.5 Km west of Ain Azel. Mineralization is represented by some ore bodies (about 25). The thickness variate from a few centimeters up to 3m. These bodies are located on the layers of dolomites and dolomitized marls and along the Kherzet Youcef fault. The geological reserves of Zinc and Lead ore are of the order of 1.6 million tons. This ore has a Pb content of 3.6% and Zn content of 18.4%. The projected annual exploitation was 100 thousand tons per year. Hydrogeological studies carried out successively (1973-1977) and (1981-1983) revealed the existence of an aquifer system located west of the Kherzet Youssef fault characterized by the presence of Karts and by strong cracking due to local brittle tectonics. These two characteristics define the filtration and storage capacity of very abundant groundwater. This groundwater represented a major handicap for mining in view of the large amounts of water that required the installation of major pumping and drainage devices. The Kherzet Youssef mine has experienced frequent flooding in the past at a time when technology could not pump efficiently. It caused the mine to close several times. In June 1990, the mine experienced a flood of great magnitude that has never been observed and despite the large installed means of pumping, this "water cost" led to the total drowning of the mine, the death of 19 workers and the stoppage of the exploitation of this deposit since. Pitting attempts were carried out with a pumping capacity of 1100 m3/h, then 1800 m3/h but without success. The interpretation of the drawdown data and field observations made it possible to conclude that this accident was only the result of the general destabilization of the massif. Our complementary work by geophysical prospecting made it possible to represent the configuration of the underground layers and demonstrate the hydrodynamic communication between the two East and West compartments of the deposit.
The Dynamic Lattice Method - Vibrations and Wave Propagation in Discontinuous and Heterogeneous Media
Authors: Sattari Amir Shoarian , Rizvi Zarghaam H. , Aji Hendrawan D. B. , Wuttke Frank
Published: November 2021

The development of a new dynamic lattice element method (dynamicLEM) as well as its application in the simulation of wave propagation in discontinuous and heterogeneous media is the focus of this research paper. The conventional static lattice models are efficient numerical methods to simulate crack initiation and propagation in cemented geomaterials. The advantage of the LEM and the developed dynamic solution, such as simulation of arbitrary crack initiation and propagation, illustration and simulation of existing inherent material heterogeneity as well as stress redistribution upon crack opening, opens a new engineering field and tool for material analysis. To realize the time dependency of the dynamic LEM, the governing Newton's second law is solved while using the Newmark-β method and implementing the non-linear Newton-Raphson Jacobian. The method validation is done according to the results of a boundary element method (BEM) in the plane P-SV-wave propagation within a plane strain domain. Further validation tests comparing the generated wave types, simulation and study of crack discontinuities as well as inherent heterogeneities in the geomaterials are conducted to illustrate the accurate applicability of the new dynamic lattice method. The results indicate that with increasing heterogeneity within the material, the wave field becomes significantly scattered and further analysis of wave fields according to the wavelength/heterogeneity ratio become indispensable. Therefore, in a heterogeneous medium, the application of continuum methods in relation to structural health monitoring should be precisely investigated and improved. The developed dynamic lattice element method is an ideal simulation tool to consider particle scale irregularities, crack distributions and inherent material heterogeneities and can be easily implemented in various engineering applications.
InflamNat: Web-Based Database and Predictor of Anti-Inflammatory Natural Products
Authors: Zhang Ruihan , Ren Shoupeng , Dai Qi , Shen Tianze , Li Xiaoli , Li Jin , Xiao Wei-Lie
Published: November 2021

Natural products (NPs) are a valuable source for anti-inflammatory drug discovery. However, they are limited by the unpredictability of the structures and functions. Therefore, computational and data-driven pre-evaluation could enable more efficient NP-inspired drug development. Since NPs possess structural features that differ from synthetic compounds, models trained with synthetic compounds may not perform well with NPs. There is also an urgent demand for well-curated databases and user-friendly predictive tools. We presented a comprehensive online web platform (InflamNat, http://www.inflamnat.com/ or http://39.104.56.4/) for anti-inflammatory natural product research. InflamNat is a database containing the physicochemical properties, cellular anti-inflammatory bioactivities, and molecular targets of 1351 NPs that tested on their anti-inflammatory activities. InflamNat provides two machine learning-based predictive tools specifically designed for NPs that (a) predict the anti-inflammatory activity of NPs, and (b) predict the compound-target relationship for compounds and targets collected in the database but lacking existing relationship data. A novel multi-tokenization transformer model (MTT) was proposed as the sequential encoder for both predictive tools to obtain a high-quality representation of sequential data. Experimental results demonstrated that the proposed predictive tools achieved the desired performance in terms of AUC.
GMCSF modulates Myeloid derived suppressor cells and Tregs activity in decompensated cirrhotic patients with sepsis
Authors: Sehgal Rashi , Maiwall Rakhi , Rajan Vijayraghavan , Islam Mojahidul , Baweja Sukriti , Kaur Navkiran , Kumar Guresh , Ramakrishna Gayatri , Sarin Shiv Kumar , Trehanpati Nirupma
Published: November 2021

Background

Decompensated cirrhosis patients are more prone to bacterial infections. Myeloid derived suppressor cells (MDSCs) expand in sepsis patients and disrupt immune cell functions. GM-CSF therapy helps in restoring immune cell functions and resolve infections. Its role in MDSCs modulation in cirrhotic with sepsis is not well understood.
Methods

164 decompensated cirrhotic; 62 without(w/o), 72 with sepsis and 30 with sepsis treated with GM-CSF and 15 healthy were studied. High-dimensional flow cytometry was performed to analyse MDSCs, monocytes, neutrophils, CD4 T-cells and Tregs at admission, day3 and 7. Ex-vivo co-cultured MDSCs with T-cells were assessed for proliferation and apoptosis of T-cells, differentiation to T-regs. Plasma factors and mRNA levels were analysed by cytokine-bead assay and qRT-PCR.
Results

Frequency of MDSCs and T-regs were significantly increased (p=0.011, and p=0.02) with decreased CD4 T-cells(p=0.01) in sepsis than without sepsis and HC (p=0.000, p=0.07 and p=0.01) at day0, and day7. In sepsis patients, MDSCs had increased IL-10, Arg1 and iNOS mRNA levels (p=0.016, p=0.049 and p=0.06). Ex-vivo co-cultured MDSCs with T-cells drove T-cell apoptosis (p=0.03, p=0.03) with decreased T-cell proliferation and enhanced FOXP3+ expression (p=0.05 and p=0.05) in sepsis compared to no sepsis at day0. Moreover, blocking the MDSCs with inhibitors suppressed FOXP3 expression. GM-CSF treatment in sepsis patients significantly decreased MDSCs and FOXP3+Tregs but increased CD4 T-cell functionality and improved survival.
Conclusion

MDSCs have immunosuppressive function by expanding FOXP3+ Tregs and inhibiting CD4+ T-cell proliferation in sepsis. GM-CSF treatment suppressed MDSCs, improved T-cell functionality and reduced Tregs in circulation.
Identification of G6PC As A Potential Prognostic Biomarker In Hepatocellular Carcinoma Based On Bioinformatics Analysis
Authors: Li Tian , Yong Liao
Published: November 2021

Background: Hepatocellular carcinoma (HCC) has high mortality and incidence rates around the world with limited therapeutic options, there is an urgent need for identification of novel therapeutic targets and biomarkers for early diagnosis and predicting patient survival with HCC. Methods: Several studies (GSE102083, GSE29722, GSE101685 and GSE112790) from the GEO database in HCC were screened and analyzed by GEO2R, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were conducted with the Database for Annotation, Visualization and Integrated Discovery (DAVID). The protein-protein interaction network (PPI) was plotted and the module analysis was performed using Search Tool for the Retrieval of Inter-acting Genes/Proteins database (STRING) and Cytoscape. The expression and survival of key genes were identified using UALCAN, Kaplan-Meier Plotter and ONCOMINE online databases, and the immune infiltration level of key genes was analyzed via TIMER database. Results: Eight key genes were finally screened out through several database analysis, and UALCAN was used to detect the expressions of CKS2 and G6PC, which were closely related to the survival of HCC patients. Further analysis on the differential expression of G6PC in multiple cancerous tumors and normal tissues revealed low expression in many solid tumors by Oncomine and TIMER. In addition, Kaplan-Meier plotter and UALCAN database analysis to access diseases prognosis suggested that low expression of G6PC was significantly associated with poor overall survival in HCC patients. Finally, TIMER database analysis showed a significant negative correlation between G6PC and infiltration levels of six kinds of immune cells. The somatic copy number alterations (SCNAs) of G6PC were associated with B cells, CD8+ T cells, CD4+ T cells, macrophages, dentritic cells and neutrophils. Conclusions: These bioinformatic data identified G6PC as a potential key gene in the diagnosis and prognosis of HCC.
Comparison of Allogenic and Autogenic Implantations of Dedifferentiated Fat Cells On Monoclonal Antibody 1-22-3-Induced Glomerulonephritis in Rats
Authors: Maruyama Takashi , Fukuda Noboru , Matsumoto Taro , Endo Morito , Utsunomiya Kei , Shimizu Satoshi , Kano Koichiro , Abe Masanori
Published: November 2021

Background
We established an adipogenic progenitor cell line derived from mature adipocytes and named these cells dedifferentiated fat (DFAT) cells, which have been shown to have characteristics very similar to those of mesenchymal stem cells (MSCs). The potential application of DFAT cells to support cell-based therapies for regenerative and immunosuppressive therapies has been suggested. The present study was designed to address beneficial ways that DFAT implantation can be used clinically as immunosuppressive therapy to treat immunological glomerulonephritis.
Methods
We evaluated distribution of DFAT cells after intravenous injection through the tail vein in Wistar rats. We examined effects of allogenic implantation of DFAT cells on BrdU incorporation into kidney from rats with monoclonal antibody (mAb) 1-22-3-induced glomerulonephritis. We compared effects of allogenic and autogenic implantations of DFAT cells on excretion of urinary protein, renal function, and glomerular and nephrotubular injuries in these rats, and serum levels of tumor necrosis factor-stimulated gene-6 (TSG-6), and expression of TSG-6 mRNA in kidney.
Results
The allogenic implantations of DFAT cells trapped in lung improved excretion of urinary protein and renal function, and significantly suppressed glomerular and nephrotubular injuries in the rats with mAb1-22-3-induced glomerulonephritis compared with the autogenic implantations. The allogenic implantation of DFAT cells increased serum levels of TSG-6 especially in mAb 1-22-3-induced glomerulonephritis and significantly increased the expression of TSG-6 mRNA in kidney compared to the autogenic implantation.
Conclusion
These findings suggest that allogenic implantation of DFAT cells could be clinically useful immunosuppressive therapy for immunological glomerulonephritis.
Mechanical and Radiation Shielding properties of CuO doped TeO2-B2O3 Glass System
Authors: NIDZAM NAZIRUL NAZRIN SHAHROL , Sharma Amandeep , Jufa M.S. , Izzaty M.K. , Boukhris Imed
Published: November 2021

A series of borotellurite glass system doped with copper oxide was synthesized using the method of melt quenching. The density of the glass decreases whereas its molar volume increases in respect to copper oxide concentration. The amorphous nature of prepared glasses was proven with the utilisation of XRD spectra. Meanwhile, the glass samples’ elastic properties are determined from the measurement of longitudinal and shear velocities using ultrasonic technique. The longitudinal modulus, shear modulus, bulk modulus and Young’s modulus will show an irregular trend that fluctuates. FLUKA Monte Carlo code is implemented to determine the shielding features at gamma energy 0.3565, 0.6616, 0.8348 and 1.3325 MeV. Linear attenuation coefficients acquired from FLUKA and XCOM are found to be in good agreement with relative difference < 1%. The glass sample B2O3TeO2CuO1 (with highest percentage of TeO2) is found to be the best shielding glass material in this study.
Mitochondrial DNA and Alzheimer's Disease: A First Case-control Study of the Tunisian Population
Authors: Salem Nesrine Ben , Boussetta Sami , de Rojas Itziar , Moreno-Grau Sonia , Montrreal Laura , Mokni Narjes , Mahmoud Imene , Younes samia , Daouassi Nizar , Frih-Ayed Mahbouba , Hammami Afef , Elgaaied Amel Ben Ammar , Ruiz Agustín , Cherni Lotfi
Published: November 2021

Background: Alzheimer’s disease (AD) is the most common neurodegenerative disorder in humans and presents a major health problem throughout the world. The etiology of AD is complex, and many factors are implicated, including mitochondria. Mitochondrial alteration has been proposed as a possible cause of AD Therefore, several studies have focused on finding an association between inherited mitochondrial DNA variants and AD onset.Methods: In this study, we looked, for the first time, for a potential association between mitochondrial haplogroups or polymorphisms and AD in the Tunisian population. We also evaluated the distribution of the major genetic risk factor for AD, the apolipoprotein E epsilon 4 (APOE ε4), in this population. In total, 159 single-nucleotide polymorphisms (SNPs) of mitochondrial DNA haplogroups were genotyped in 254 individuals (58 patients and 196 controls). An additional genotyping of APOE ε4 was performed.Results: No significant association between mitochondrial haplogroups and AD was found. However, two individual SNPs, A5656G (p = 0.03821, OR = 10.46) and A13759G (p = 0.03719, OR = 10.78), showed a significant association with AD. APOE 4 was confirmed as a risk factor for AD (p = 0.000014). Conclusion: Our findings may confirm the absence of a relation between mitochondrial haplogroups and AD and support the possible involvement of some inherited variants in the pathogenicity of AD.
No Association of Eight TNFAIP3 Single Nucleotide Variants to Rheumatoid Arthritis in Native Mexicans
Authors: Aranda-Uribe Iván Sammir , Ramírez-Bello Julián , Victoria-Acosta Georgina , Barbosa-Cobos Rosa Elda , Moreno José
Published: November 2021

Rheumatoid arthritis (RA) is a chronic inflammatory disease of autoimmune origin associated with many genetic traits, including genes related to the control of inflammation. The A20 protein, encoded by the TNFAIP3 gene, is a negative regulator of NF-kB-mediated inflammation. Several single nucleotide variants (SNVs) of TNFAIP3 are associated with susceptibility to RA in different ethnic groups, but not in Chileans, the only Latin Americans studied thus far. Objective. To examine the association of eight TNFAIP3 SNVs, four of them not previously studied, with RA in Mexican patients. Materials . We studied 471 RA patients (ACR-EULAR 2010) and eight TNFAIP3 SNVs: including, rs10499194C/T, rs6920220G/A and rs2230926T/G, which are associated with RA in European and Asian patients, in addition to rs373421182G/C, rs139054966T/G, rs5029924C/T, rs59693083A/G and rs61593413T/A, not previously examined in RA. All SNVs were evaluated by means of an allelic discrimination assay and TaqMan probes. Results. The allelic and genotypic frequencies of all SNVs examined were similar between cases and controls, and none of them was associated with RA under the allelic, codominant, dominant, and recessive models was observed. Conclusion. Our data indicate that the TNFAIP3 SNVs evaluated herein are not risk factors for RA in Mexican subjects.
Grain yield stability analysis using AMMI and GGE biplot models in different breeding zones of Bangladesh
Authors: Khatun Marium , Islam A. K. M. Aminul , Islam M. Rafiqul , Khan M. A. Rahman , Hossain M. Kamal
Published: November 2021

During the 2018-2019 Boro season (dry season), 70 rice genotypes were examined with alpha lattice experimental design with the goal of measuring grain yield stability analysis. Results indicated that AMMI analysis explained 100% of the G×E variance, while captured 81.74% variance. Based on the GGE and AMMI analysis, the most stable and high yielding genotype was identified G41 followed by G22, G26, G58, G24 and G61. The AMMI 1 biplot analysis revealed that the first primary component of interaction (IPC1) factor was responsible for 64.2 % variation due to G × E interaction. On other hand, the second primary component (PC2) factor accounted for 35.8% variation of the G × E interaction. These two-primary component (PC1 and PC2), all together accounted for 100% variation of the G × E interaction. The contribution of G68 was highest to the interaction followed by G70, G58, G42, G61, G45, G38, G14, G33, G60, G53, and G9. Best environment analysis indicated that the ranking was Rajshahi < Gazipur < Cumilla. GGE biplot analysis accounted for 81.74% variation comprising two principal components PC1 and PC2 with 45.62% and 36.12% variations respectively. Rajshahi was more stable than Gazipur. Based on environment analysis genotypes, G22, G26, G58, and G44 can be recommended as best stable genotypes that breeding zone. However, the genotype G61 was identified adapted to Cumilla breeding zone.
Comparison Efficacy of Ultrasound-Guided Needle Release Plus Corticosteroid Injection and Mini-Open Surgery in Patients with Carpal Tunnel Syndrome
Authors: Zeng Zeng , Lin Nan , Chen Cong-Xian
Published: November 2021

This retrospective study was to compare clinical outcomes of ultrasound-guided needle release with corticosteroid injection versus mini-open surgery in patients with carpal tunnel syndrome (CTS). From January 2020 to December 2020, 40 patients (40 wrists) with CTS were analyzed in this study. Diagnosis was based on clinical symptoms, electro-physiological and ultrasound imaging. 20 wrists were treated with ultrasound-guided needle release plus corticosteroid injection (Group A) and other 20 wrists went mini-open surgery (Group B). We evaluated Boston carpal tunnel questionnaire, electrophysiological parameters (distal motor latency, sensory conduction velocity and sensory nerve action potential of median nerve) and ultrasound parameters (cross-sectional area, flattening ratio and the thicknesses of transverse carpal ligament) both before and 3 months after treatment. Total treatment cost, duration of treatment, healing time and complications were also recorded for two groups. Boston carpal tunnel questionnaire, electrophysiologic and ultrasound outcomes at preoperatively and 3 months postoperatively had significant difference for each group (each P༜0.05). There were no complications such as infection, haemorrhage, vascular, nerve or tendon injuries in both groups. Ultrasound-guided needle release and mini-open surgery are both effective measures in treating CTS patients. Ultrasound-guided needle release plus corticosteroid injection provides smaller incision, less cost, less time of treatment and faster recovery compared with mini-open surgery. Ultrasound-guided needle release plus corticosteroid injection is better for clinical application.
An Immune Algorithm Based on Entropy For Computing Offloading Problem in Edge Computing Scenes
Authors: Sifeng ZHU , Jianghao CAI , Enlin SUN , Qinghua ZHANG
Published: November 2021

By offloading computing tasks to the edge of mobile network, mobile edge computing(MEC) solves the shortcomings of mobile devices in computing capacity and storage capacity. In order to reduce the computing delay of mobile terminal devices and the degree of workload balancing in MEC servers, a computing offloading solution based on information entropy immune algorithm is proposed. Firstly, the system model, delay model, load model and objective optimization model are constructed. Then, a task offloading scheme based on immune optimization is proposed .Finally, this scheme is compared with the Benchmark scheme and the offloading scheme in the literature. Simulation experiments show that the proposed scheme can effectively reduce the average offloading delay, and can effectively balance the load between MEC servers. At the same time, compared with the schemes in the literature, the performance of the scheme is improved to a certain extent.
Additively Manufactured Microfluidic Enzymatic Biofuel Cell with Comb -like Bioelectrodes
Authors: Sivakumar Jayapiriya Umarani , Goel Sanket
Published: November 2021

The authors have requested that this preprint be removed from Research Square.