bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Combinations of Untranslated-Region Sequences on Translation of mRNA
Authors: Kirshina Anna , Vasileva Olga , Kunyk Dmitry , Seregina Kristina , Muslimov Albert , Ivanov Roman , Reshetnikov Vasiliy
Published: November 2023

mRNA-based therapeutics have been found to be a promising treatment strategy in immunotherapy, gene therapy, and cancer treatments. Effectiveness of mRNA therapeutics depends on the level and duration of a desired protein’s expression, which is determined by various cis- and trans-regulatory elements of the mRNA. Sequences of 5′ and 3′ untranslated regions (UTRs) are responsible for translational efficiency and stability of mRNA. An optimal combination of the regulatory sequences allows researchers to significantly increase the target protein’s expression. Using both literature data and previously obtained experimental data, we chose six sequences of 5′UTRs (adenoviral tripartite leader [TPL], HBB, rabbit β-globin [Rabb], H4C2, Moderna, and Neo2) and five sequences of 3′UTRs (mtRNR-EMCV, mtRNR-AES, mtRNR-mtRNR, BioNTech, and Moderna). By combining them, we constructed 30 in vitro transcribed RNAs encoding firefly luciferase with various combinations of 5′- and 3′UTRs, and the resultant bioluminescence was assessed in the DC2.4 cell line at 4, 8, 24, and 72 h after transfection. The cellular data enabled us to identify the best seven combinations of 5′- and 3′UTRs, whose translational efficiency was then assessed in BALB/c mice. Two combinations of 5′- and 3′UTRs (5′Rabb-3′mtRNR-EMCV and 5′TPL-3′Biontech) led to the most pronounced increase in the luciferase amount in the in vivo experiment in mice. Subsequent analysis of the stability of the mRNA indicated that the increase in luciferase expression is explained primarily by the efficiency of translation, not by the number of RNA molecules. Altogether, these findings suggest that 5′UTR-and-3′UTR combinations 5′Rabb-3′mtRNR- EMCV and 5′TPL-3′Biontech lead to high expression of target proteins and may be considered for use in preventive and therapeutic modalities based on mRNA.
The Potential Role of Ionic Liquid as a Multifunctional Dental Biomaterial
Authors: Hossain Md Iqbal , Shams Abdullah Bin , Das Gupta Shuvashis , Blanchard Gary J. , Mobasheri Ali , Hoque Apu Ehsanul
Published: November 2023

In craniofacial research and routine dental clinical procedures, multifunctional materials with antimicrobial properties are in constant demand. Ionic liquids (ILs) are one such multifunctional intelligent material. Over the last three decades, ILs have been explored for different biomedical applications due to their unique physical and chemical properties, high task specificity, and sustainability. Their stable physical and chemical characteristics and extremely low vapor pressure make them suitable for various applications. Their unique properties, such as density, viscosity, and hydrophilicity/hydrophobicity, may provide higher performance as a potential dental material. ILs have functionalities for optimizing dental implants, infiltrate materials, oral hygiene maintenance products, and restorative materials. They also serve as sensors for dental chairside usage to detect oral cancer, periodontal lesions, breath-based sobriety, and dental hard tissue defects. With further optimization, ILs might also make vital contributions to craniofacial regeneration, oral hygiene maintenance, oral disease prevention, and antimicrobial materials. This review explores the different advantages and properties of ILs as possible dental material.
On the Role of Iron in Idiopathic Parkinson’s Disease
Authors: Huenchuguala Sandro , Segura-Aguilar Juan
Published: November 2023

The transition metal characteristics of iron allow it to play a fundamental role in several essential aspects of human life such as the transport of oxygen through hemoglobin or the transport of electrons in the mitochondrial respiratory chain coupled to the synthesis of ATP. However, an excess or deficiency of iron is related to certain pathologies. The maintenance of iron homeostasis is essential to avoid certain pathologies related to iron excess or deficiency. The existence of iron deposits in postmortem tissues of Parkinson’s patients has been interpreted as evidence that iron plays a fundamental role in the degenerative process of the nigrostriatal system in this disease. The use of iron chelators has been successful in the treatment of diseases such as transfusion-dependent thalassemia and pantothenate kinase-associated neurodegeneration. However, a clinical study with the iron chelator deferiprone in patients with Parkinson’s disease has not shown positive effects but rather worsened clinical symptoms. This suggests that iron may not play a role in the degenerative process of Parkinson’s disease.
PSMA-Expression Is Highly Associated with Histological Subtypes of Renal Cell Carcinoma: Potential Implications for Theranostic Approaches
Authors: Bui Vinh Ngoc , Unterrainer Lena M. , Brendel Matthias , Kunte Sophie C. , Holzgreve Adrien , Allmendinger Fabian , Bartenstein Peter , Klauschen Frederick , Unterrainer Marcus , Staehler Michael , Ledderose Stephan
Published: November 2023

In renal cell carcinoma (RCC), accurate imaging methods are required for treatment planning and response assessment to therapy. In addition, there is an urgent need for new therapeutic options, especially in metastatic RCC. One way to combine diagnostics and therapy in a so-called theranostic approach is the use of radioligands directed against surface antigens. For instance, radioligands against prostate-specific membrane antigen (PSMA) have already been successfully used for diagnosis and radionuclide therapy of metastatic prostate cancer. Recent studies have demonstrated that PSMA is expressed not only in prostate cancer but also in the neovasculature of several solid tumors, which has raised hopes to use PSMA-guided theranostic approaches in other tumor entities, too. However, data on PSMA expression in different histopathological subtypes of RCC are sparse. Because a better understanding of PSMA expression in RCC is critical to assess which patients would benefit most from theranostic approaches using PSMA-targeted ligands, we investigated the expression pattern of PSMA in different subtypes of RCC on protein level. Immunohistochemical staining for PSMA was performed on formalin-fixed, paraffin-embedded archival material of major different histological subtypes of RCC (clear cell RCC (ccRCC)), papillary RCC (pRCC) and chromophobe RCC (cpRCC). The extent and intensity of PSMA staining were scored semi-quantitatively and correlated with the histological RCC subtypes. Group comparisons were calculated with the Kruskal–Wallis test. In all cases, immunoreactivity was detected only in the tumor-associated vessels and not in tumor cells. Staining intensity was the strongest in ccRCC, followed by cpRCC and pRCC. ccRCC showed the most diffuse staining pattern, followed by cpRCC and pRCC. Our results provide a rationale for PSMA-targeted theranostic approaches in ccRCC and cpRCC.
The Outcomes of Liver Transplantation in Severe Metabolic Dysfunction-Associated Steatotic Liver Disease Patients
Authors: Paklar Natasa , Mijic Maja , Filipec-Kanizaj Tajana
Published: November 2023

The increasing prevalence of diabetes mellitus, obesity, and metabolic syndrome in the population can lead to metabolic dysfunction-associated steatohepatitis (MASH) and metabolic dysfunction-associated steatotic liver disease (MASLD). In Western industrialized countries, this has become a major problem with significant socioeconomic impacts. MASH is now a leading cause of liver transplantation (LT), especially in developed countries. However, the post-transplant outcomes of such patients are a major concern, and published data are limited and extremely variable. In this article, we discuss graft and patient survival after LT, complications, the recurrence of MASH, and MASH appearing de novo after transplantation. Recent studies suggest that patients with MASH have slightly worse short-term survival, potentially due to increased cardiovascular mortality. However, most studies found that longer-term outcomes for patients undergoing LT for MASH are similar or even better than those for other indications. Hepatocellular carcinoma due to MASH cirrhosis also has similar or even better outcomes after LT than other etiologies. In conclusion, we suggest questions and topics that require further research to enhance healthcare for this growing patient population.
Carfilzomib Mitigates Lipopolysaccharide/D-Galactosamine/Dimethylsulfoxide-Induced Acute Liver Failure in Mice
Authors: Alhareth Dhafer , Alanazi Abdulrazaq , Alanazi Wael , Ansari Mushtaq , Nagi Mahmoud , Ahmad Sheikh , Attia Mohamed , Nadeem Ahmed , Bakheet Saleh , Attia Sabry
Published: November 2023

Acute liver failure (ALF) is a disease accompanied by severe liver inflammation. No effective therapy is available yet apart from liver transplantation; therefore, developing novel treatments for ALF is urgently required. Inflammatory mediators released by NF-кB activation play an essential role in ALF. Proteasome inhibitors have many medical uses, such as reducing inflammation and NF-кB inhibition, which are believed to account for most of their repurposing effects. This study was undertaken to explore the possible protective effects and the underlying mechanisms of carfilzomib, a proteasome inhibitor, in a mouse model of ALF induced by lipopolysaccharide/D-galactosamine/dimethylsulfoxide (LPS/GalN/DMSO). Carfilzomib dose-dependently protected mice from LPS/GalN/DMSO-induced liver injury, as indicated by the decrease in serum alanine aminotransferase and aspartate aminotransferase levels. LPS/GalN/DMSO increased TNF-α, NF-кB, lipid peroxidation, NO, iNOS, cyclooxygenase-II, myeloperoxidase, and caspase-3 levels. Carfilzomib administration mitigated LPS/GalN/DMSO-induced liver damage by decreasing the elevated levels of TNF-α, NF-кB, lipid peroxidation, nitric oxide, iNOS, cyclooxygenase-II, myeloperoxidase, caspase-3, and histopathological changes. A restored glutathione level was also observed in the carfilzomib-treated LPS/GalN/DMSO mice. Our results demonstrate that carfilzomib protects against LPS/GalN/DMSO-induced ALF by inhibiting NF-кB, decreasing inflammatory mediators, oxidative/nitrosative stress, neutrophil recruitment, and apoptosis, suggesting that carfilzomib may be a potential therapeutic agent for ALF.
Photodynamic Therapy in Pigmented Basal Cell Carcinoma—A Review
Authors: Mazur Ewelina , Kwiatkowska Dominika , Reich Adam
Published: November 2023

This review summarizes the effectiveness of photodynamic therapy (PDT) in the treatment of the pigmented subtype of basal cell carcinoma (BCC) based on the current literature. PDT is a light-activated treatment, non-invasive, that selectively destroys tumor cells and tissues via the interaction of a photosensitizer, light, and molecular oxygen. It can induce cancer cell death through direct tumor vascular damage or via the induction of immune response. However, human skin is also an absorption and scattering medium since it contains hemoglobin and melanin that act as chromophores. Eumelanin can be considered a light-absorber and an intracellular antioxidant that can neutralize PDT-induced ROS and, therefore, decrease PDT success. Various factors, including tumor depth, the degree of pigmentation in malignant cells, and the individual’s skin phototype, can impact the outcome of this intricate biochemical process. It has been widely recognized that PDT exhibits limited efficacy in the treatment of pigmented lesions. However, new combination techniques such as curettage or debulking before PDT show promising results in the treatment of pigmented BCC.
GSK2606414 Sensitizes ABCG2-Overexpressing Multidrug-Resistant Colorectal Cancer Cells to Chemotherapeutic Drugs
Authors: Yu Ze-Zhong , Xu Bu-Qing , Wang Ying-Ying , Zhang Peng-Wei , Shu Yu-Bin , Shi Zhi
Published: November 2023

Colorectal cancer is a common malignant tumor. A major factor in the high mortality rate of colorectal cancer is the emergence of multidrug resistance (MDR). Overexpression of the ABCG2 gene in cancer cells directly leads to MDR. Finding new inhibitors of ABCG2 may be an effective way to overcome drug resistance. We found that the compound GSK2606414 enhanced the sensitivity of the ABCG2 substrate to the chemotherapeutic drugs mitoxantrone and doxorubicin in ABCG2-overexpressing multidrug-resistant colorectal cancer cells by increasing their intracellular accumulation without affecting the protein expression of ABCG2. Molecular docking experiments predicted that GSK2606414 could stably bind in the drug-binding pocket of ABCG2. In conclusion, GSK2606414 can sensitize ABCG2-overexpressed multidrug-resistant colorectal cancer cells to chemotherapy drugs and can be used as a potential inhibitor of ABCG2.
Deep Convolutional Neural Network with Symbiotic Organism Search-Based Human Activity Recognition for Cognitive Health Assessment
Authors: Alonazi Mohammed , Alshahrani Haya Mesfer , Kouki Fadoua , Almalki Nabil Sharaf , Mahmud Ahmed , Majdoubi Jihen
Published: November 2023

Cognitive assessment plays a vital role in clinical care and research fields related to cognitive aging and cognitive health. Lately, researchers have worked towards providing resolutions to measure individual cognitive health; however, it is still difficult to use those resolutions from the real world, and therefore using deep neural networks to evaluate cognitive health is becoming a hot research topic. Deep learning and human activity recognition are two domains that have received attention for the past few years. The former is for its relevance in application fields like health monitoring or ambient assisted living, and the latter is due to their excellent performance and recent achievements in various fields of application, namely, speech and image recognition. This research develops a novel Symbiotic Organism Search with a Deep Convolutional Neural Network-based Human Activity Recognition (SOSDCNN-HAR) model for Cognitive Health Assessment. The goal of the SOSDCNN-HAR model is to recognize human activities in an end-to-end way. For the noise elimination process, the presented SOSDCNN-HAR model involves the Wiener filtering (WF) technique. In addition, the presented SOSDCNN-HAR model follows a RetinaNet-based feature extractor for automated extraction of features. Moreover, the SOS procedure is exploited as a hyperparameter optimizing tool to enhance recognition efficiency. Furthermore, a gated recurrent unit (GRU) prototype can be employed as a categorizer to allot proper class labels. The performance validation of the SOSDCNN-HAR prototype is examined using a set of benchmark datasets. A far-reaching experimental examination reported the betterment of the SOSDCNN-HAR prototype over current approaches with enhanced precision of 86.51% and 89.50% on Penn Action and NW-UCLA datasets, respectively.
Advanced Biomimetic Multispectral Curved Compound Eye Camera for Aerial Multispectral Imaging in a Large Field of View
Authors: Zhang Yuanjie , Xu Huangrong , Liu Yiming , Zhou Xiaojun , Wu Dengshan , Yu Weixing
Published: November 2023

In this work, we demonstrated a new type of biomimetic multispectral curved compound eye camera (BM3C) inspired by insect compound eyes for aerial multispectral imaging in a large field of view. The proposed system exhibits a maximum field of view (FOV) of 120 degrees and seven-waveband multispectral images ranging from visible to near-infrared wavelengths. Pinhole imaging theory and the image registration method from feature detection are used to reconstruct the multispectral 3D data cube. An airborne imaging experiment is performed by assembling the BM3C on an unmanned aerial vehicle (UAV). As a result, radiation intensity curves of several objects are successfully obtained, and a land type classification is performed using the K-means method based on the aerial image as well. The developed BM3C is proven to have the capability for large FOV aerial multispectral imaging and shows great potential applications for distant detecting based on aerial imaging.
Recent Research Progress in Fluorescent Probes for Detection of Amyloid-β In Vivo
Authors: Zhang Zhen-Yu , Li Ze-Jun , Tang Ying-Hao , Xu Liang , Zhang De-Teng , Qin Tian-Yi , Wang Ya-Long
Published: November 2023

Alzheimer’s disease (AD) is a neurodegenerative disease. Due to its complex pathological mechanism, its etiology is not yet clear. As one of the main pathological markers of AD, amyloid-β (Aβ) plays an important role in the development of AD. The deposition of Aβ is not only related to the degeneration of neurons, but also can activate a series of pathological events, including the activation of astrocytes and microglia, the breakdown of the blood–brain barrier, and the change in microcirculation, which is the main cause of brain lesions and death in AD patients. Therefore, the development of efficient and reliable Aβ-specific probes is crucial for the early diagnosis and treatment of AD. This paper focuses on reviewing the application of small-molecule fluorescent probes in Aβ imaging in vivo in recent years. These probes efficiently map the presence of Aβ in vivo, providing a pathway for the early diagnosis of AD and providing enlightenment for the design of Aβ-specific probes in the future.
Defining Collision Risk: Lesser Flamingo Phoeniconaias minor Power Line Collision Sensitivity and Exposure for Proactive Mitigation
Authors: Pretorius Mattheuns D. , Galloway-Griesel Tamsyn L. , Leeuwner Lourens , Michael Michael D. , Durgapersad Kaajial , Chetty Kishaylin
Published: November 2023

Lesser Flamingos Phoeniconaias minor regularly collide with power lines in South Africa. Attaching light-emitting markers to overhead wires seems to be an effective mitigation measure; however, the cost of these devices is prohibitive of large-scale installation. Spatial predictions about flamingo collision risk are thus important for achieving efficient and effective proactive mitigation. In this study, collision risk was defined as a combination of factors related to threat exposure. A habitat suitability index was developed according to changes in surface water occurrence and Chlorophyll-a concentrations, which proved accurate in predicting Lesser Flamingo occurrence. Habitat suitability, and three other species and threat exposure variables, were then used in logistic regression models predicting the occurrence of historic collisions. The most parsimonious model included habitat suitability and flight height. Flamingos were only at risk of collision with power lines when flying lower than 50 m and within 3 km from the water’s edge. High-risk power line sections were thus identified from 3 km buffers around waterbodies ranked according to habitat suitability, which significantly reduced the number of power line spans predicted for proactive marking. While our models indicated that aspects related to exposure were important for predicting flamingo power line collisions, aspects related to sensitivity (e.g., nocturnal behavior) must also guide the choice of mitigation.
Developing STEM Career Identities among Latinx Youths: Collaborative Design, Evaluations, and Adaptations during COVID-19
Authors: Park Chong Myung , Donnelly Hayoung Kim , Rodriguez Angelica , Esquivel Luis , Nardi Cecilia , Trunfio Paul , Oliver-Davila Alexandra , Howard Kimberly A. S. , Solberg V. Scott H.
Published: November 2023

In response to the low representation of Latinx adults in STEM occupations, this community-based participatory action research study aims to increase the number of middle school youths developing STEM career identities and entering high school with the intention to pursue STEM careers. The students were provided with summer and after-school activities focusing on network science and career development curricula. Using a quasi-experimental pretest–posttest design and career narratives, this study examined the changes in STEM and career self-efficacy, as well as career identity. The results show improvements in self-efficacy, an increased number of youths with intentions of pursuing future STEM career opportunities, and deeper reflections on their talents and skills after program participation. This paper also describes the program development and implementation in detail, as well as the adaptations that resulted from COVID-19, for scholars and educators designing similar programs. This study provides promising evidence for the quality of STEM and career development lessons in supporting the emergence of a STEM career identity and self-efficacy.
Predictors of Vaccination Intentions and Behaviour during the COVID-19 Pandemic in Italy
Authors: Nerini Amanda , Duradoni Mirko , Matera Camilla , Guazzini Andrea , Paradisi Monica , Schembri Adriele
Published: November 2023

The present research aimed at understanding individuals’ vaccination intentions and protective behaviours against COVID-19 through two different studies. In Study 1 (N = 213, 73% women; mean age = 24.03) the Protection Motivation Theory model was tested considering the fear of COVID-19 as a possible mediator between threat appraisal (in terms of both health and social life) and intentions to get vaccinated when the vaccination was not yet available. Study 2 (N = 1111, 68.9% women; mean age = 38.33) was conducted when the vaccine became available for the entire population. Through this study, by adopting the 5C model of vaccine hesitancy as a theoretical framework, we aimed to understand how people who got vaccinated and the ones who did not differed, considering fear of vaccination, personality and vaccination hesitancy. In Study 1, social limitations, perceived severity and COVID-19 perceived vulnerability were significantly and positively related to fear of COVID-19. Contrary to what we expected, fear of COVID-19 was not a significant predictor of vaccination intention, which was predicted by both response efficacy and self-efficacy. In Study 2, in line with previous studies, vaccine hesitancy was negatively related to vaccination. More specifically, the social-oriented dimension of collective responsibility was the strongest predictor of effective behaviour. Our findings provide insights into the complexity of vaccine acceptance and emphasise the need for targeted interventions to promote vaccination and mitigate the spread of infectious diseases.
Habitual Mask Wearing as Part of COVID-19 Control in Japan: An Assessment Using the Self-Report Habit Index
Authors: Li Tianwen , Fujimoto Marie , Hayashi Katsuma , Anzai Asami , Nishiura Hiroshi
Published: November 2023

Although the Japanese government removed mask-wearing requirements in 2023, relatively high rates of mask wearing have continued in Japan. We aimed to assess psychological reasons and the strength of habitual mask wearing in Japan. An Internet-based cross-sectional survey was conducted with non-random participant recruitment. We explored the frequency of mask usage, investigating psychological reasons for wearing masks. A regression analysis examined the association between psychological reasons and the frequency of mask wearing. The habitual use of masks was assessed in the participant’s most frequently visited indoor space and public transport using the self-report habit index. The principal component analysis with varimax rotation revealed distinct habitual characteristics. Among the 2640 participants surveyed from 6 to 9 February 2023, only 4.9% reported not wearing masks at all. Conformity to social norms was the most important reason for masks. Participants exhibited a slightly higher degree of habituation towards mask wearing on public transport compared to indoor spaces. The mask-wearing rate was higher in females than in males, and no significant difference was identified by age group. Daily mask wearing in indoor spaces was characterized by two traits (automaticity and behavioral frequency). A high mask-wearing frequency has been maintained in Japan during the social reopening transition period. Mask wearing has become a part of daily habit, especially on public transport, largely driven by automatic and frequent practice.
Relationship between Physical Fitness and Cyberbullying Patterns (Cybervictimization and Cyberperpetration) in Spanish Adolescents
Authors: Benítez-Sillero Juan de Dios , Corredor-Corredor Diego , Martínez-Aranda Luis Manuel , Abellán-Aynés Oriol , Portela-Pino Iago , Raya-González Javier
Published: November 2023

(1) Background: Cyberbullying is a growing problem among adolescents, and deeper knowledge of this phenomenon could facilitate the implementation of adequate prevention and intervention strategies. Therefore, the aim of this study was to analyze the relationships between victimization and aggression patterns in cyberbullying and physical fitness levels in Spanish adolescents. (2) Methods: A total of 741 adolescents aged 12 to 19 years from two high schools in Andalusia, Spain (mean = 14.52 ± 1.96 years; 50.9% girls, 49.1% boys) participated in the study. The participants underwent the EUROFIT battery test and completed the Spanish version of the European Cyberbullying Intervention Project Questionnaire (ECIPQ) scale. T-test, bivariate correlations and a linear regression analysis were used for statistical analysis. (3) Results: The study results indicated positive relationships between cyberbullying patterns, especially cybervictimization, and age, as well as several physical fitness measures, including BMI, sit-ups, sit-and-reach, and handgrip tests. On the other hand, cyberperpetration was positively related only to age and the specific grip strength test. Further statistical analysis revealed that cybervictimization was primarily influenced by age, while cyberperpetration was mainly influenced by age and performance in a functional test (sit-and-reach). (4) Conclusions: Cyberbullying roles, both as victims and aggressors, may not be strongly influenced by isolated physical fitness factors. Thus, in order to reach a more comprehensive understanding and better explanations of individual involvement in cyberbullying behavior, future studies should analyze psychological and social factors along with the variables considered in this study.
Analysis of Critical Project Success Factors—Sustainable Management of the Fast-Track Construction Industry
Authors: Naji Khalid K. , Gunduz Murat , Adalbi Mohamed
Published: November 2023

Fast-track construction has recently become the prevailing construction approach globally. It enables owners and developers to make rapid returns on investment through shortened construction periods. This strategy has many effects on the industry; therefore, four groups of factors were studied in this project: (1) financial, (2) logistics and finance, (3) management, and (4) legal. A 22-question survey was distributed to 155 professionals in the construction industry, who evaluated the impact of the stated factors on a 5-point scale. The results revealed a high level of consistency determined through Cronbach’s alpha, and a positive correlation was found by Spearman’s rank coefficient. The Relative Importance Index was used to rank the factors based the evaluation by the professionals, resulting in the following impact ranking: (1) poor communication among design and construction teams, (2) large amounts of rework, (3) low quality of work by the contractor, (4) design errors, (5) late or insufficient payment according to terms agreed with the client, and (6) unavailability of materials in the market. By providing a quantitative RII model to evaluate fast-track project management performance with the use of corresponding performance indicators, this study will benefit industry practitioners and researchers as it identifies the most significant factors that impact fast-tract project management performance.
Definition of Compliance Criterion Weights for Bridge Construction Method Selection and Their Application in Real Projects
Authors: Tegos Nikolaos , Papadopoulos Ilias , Aretoulis Georgios
Published: November 2023

The main research purpose of the present paper is the establishment of certain compliance criteria, applied for the selection of the most appropriate, per case, bridge construction method, as well as the definition of the weights of these criteria. The five basic concrete bridge construction methods considered in this study are: Cast-in-place, Precast I-Girder, Incremental Launching, Advanced Shoring, and Balanced Cantilever. In this context, the choice of construction method in a concrete road bridge project is proposed based on seven compliance criteria which are: safety, economy, durability, construction speed, serviceability, aesthetics, and environmental harmonization. The inclusion of all these criteria is achieved via the decision-making tool of multi-criteria analysis. A notable innovation of the current study is that road bridges are divided into three categories (bridges for highways, national roads, and provincial roads), in accordance with the importance of the road that contains them. Thus, three different sets of weights of criteria are calculated, corresponding to each bridge category. The research method used for this purpose was a structured questionnaire that was distributed to a large number of selected experts in the field of bridges, who come either from academia or the construction industry. The research results showed that the criteria of safety and economy are the most significant according to the experts, while aspects such as the correlations between experts’ profile and their weights were also considered. Finally, the derived criterion weights were applied to two case studies of real bridge projects in Greece.
Indoor Air Quality in Cob Buildings: In Situ Studies and Artificial Neural Network Modeling
Authors: Touati Karim , Benzaama Mohammed-Hichem , El Mendili Yassine , Le Guern Malo , Streiff François , Goodhew Steve
Published: November 2023

Knowledge of indoor air quality (IAQ) in cob buildings during the first few months following their delivery is of vital importance in preventing occupants’ health problems. The present research focuses on evaluating IAQ in cob buildings through a prototype built in Normandy, France. To achieve this, the prototype was equipped with a set of sensors to monitor various parameters that determine indoor and outdoor air quality. These parameters include relative humidity (RH), carbon dioxide (CO2), nitrogen dioxide (NO2), ozone (O3), particulate matter (PM1 and PM10), and volatile organic compounds (VOCs). The obtained experimental results indicate that, overall, there is good indoor air quality in the prototype building. However, there are some noteworthy findings, including high indoor RH and occasional spikes in CO2, PM1, PM10, and VOCs concentrations. The high RH is believed to be a result of the ongoing drying process of the cob walls, while the peaks in pollutants are likely to be attributed to human presence and the earthen floor deterioration. To ensure consistent good air quality, this study recommends the use of a properly sized Controlled Mechanical Ventilation system. Additionally, this study explored IAQ in the cob building from a numerical perspective. A Long Short-Term Memory (LSTM) model was developed and trained to predict pollutant concentrations inside the building. A validation test was conducted on the CO2 concentration data collected on-site, and the results indicated that the LSTM model has accurately predicted the evolution of CO2 concentration within the prototype building over an extended period.
Ground Vibration Response to Vibratory Sheet Pile Driving and Extraction
Authors: Guo Feng , Jia Cangqin , Li Zuochun , Wang Yajian , Huang Feng , Wang Guihe , Yang Shuo
Published: November 2023

Sheet piles are extensively used as foundation structures in urban environments. However, the vibrations associated with sheet pile construction can potentially adversely affect existing buildings, as well as cause discomfort to nearby residents. This study aims to analyze ground vibration response during the driving and extraction of sheet piles. To this end, field tests of U-shaped sheet piles were conducted in Beijing silty clay, during which ground vibrations in the near-field were monitored. Subsequently, a numerical model was developed using the coupled Eulerian–Lagrangian method to simulate the pile–soil interaction characteristics and to investigate ground vibration intensity in the far-field. The research results indicate that the ground vibration response modes during the driving and extraction of sheet piles are distinctly different. Due to the entry effect, the critical depth during pile driving typically occurs in shallow soil layers, while during pile extraction, the critical depth generally corresponds to the pile’s embedded depth to overcome the soil locking effect. Ground vibrations rapidly decrease in the near-field (<6 m), while in the far-field (>6 m), the attenuation rate significantly slows down. Vibrations can be widely perceived by residents at radial distances of less than 12 m. Through a systematic assessment, it was concluded that sheet pile construction is unlikely to directly damage surrounding buildings but may inconvenience nearby residents. Additionally, a parametric analysis of the vibration source revealed that appropriately adjusting the driving frequency and amplitude can effectively reduce vibration levels.
The Risk of Venous Thromboembolism in Neuroendocrine Neoplasms
Authors: Wójcik-Giertuga Monika , Malczewska-Herman Anna , Kos-Kudła Beata
Published: November 2023

Neuroendocrine neoplasms (NENs) differ from other malignancies in their ability to produce hormones and biogenic amines, as well as offer a better prognosis in well-differentiated tumors. There are no definite data on the occurrence of thromboembolic events in NENs and no recommendations regarding the use of antithrombotic prophylaxis in this group. Accurate assessment of the thromboembolic risk in NENs represents an important issue, in order to reduce morbidity and mortality due to complications of VTE. The aim of this work was to review the occurrence of thromboembolic events in NENs and the use of antithrombotic prophylaxis in this group. A total of 28 studies identified on PubMed were analyzed. NENs, especially of pancreatic primary, exhibit an increased thrombotic risk. Atypical VTE locations are quite common in NENs. Hormonally active NENs are associated with a significantly increased thromboembolic risk. Further studies in NENs are needed to evaluate the parameters of coagulation and fibrinolysis as predictive biomarkers for VTE complications.
Organically Functionalized Porous Aluminum Phosphonate for Efficient Synthesis of 5-Hydroxymethylfurfural from Carbohydrates
Authors: Mitra Riddhi , Malakar Bhabani , Bhaumik Asim
Published: November 2023

Naturally occurring fossil fuels are the major resource of energy in our everyday life, but with the huge technological development over the years and subsequent energy demand, the reserve of this energy resource is depleting at an alarming rate, which will challenge our net energy resources in the near future. Thus, an alternative sustainable energy resource involving biomass and bio-refinery has become the most emerging and demanding approach, where biofuels can be derived effectively from abundant biomass via valuable chemical intermediates like 5-hydroxymethylfurfural (5-HMF). 5-HMF is a valuable platform chemical for the synthesis of fuel and fine chemicals. Herein, we report the synthesis of the organically functionalized porous aluminum phosphonate materials: Ph-ALPO-1 in the absence of any template and Ph-ALPO-2 by using 1,3-diaminopropane-N,N,N′,N′-tetraacetic acid as a small organic molecule template and phenylphosphonic acid as a phosphate source. These hybrid phosphonates are used as acid catalysts for the synthesis of 5-HMF from carbohydrates derived from biomass resources. These Ph-ALPO-1 and Ph-ALPO-2 materials catalyzed the dehydration of fructose to 5-HMF with total yields of 74.6% and 90.7%, respectively, in the presence of microwave-assisted optimized reaction conditions.
S-Scheme WO3/SnIn4S8 Heterojunction for Water Purification: Enhanced Photocatalytic Performance and Mechanism
Authors: Xu Pingfan , Zhang Runqiu , Gong Jiarong , Luo Yaofa , Zhuang Yihua , Zhang Peikun
Published: November 2023

Photocatalysis is a promising technology for removing micropollutants in water. However, developing efficient and stable catalysts remains a challenge. In this work, a novel step-scheme (S-scheme) heterojunction of WO3/SnIn4S8 (WSI) was constructed through the combined process of in situ precipitation with hydrothermal synthesis to simultaneously realize photocatalytic degradation of bisphenol A(BPA) and reduction of Cr(VI) in contaminated water. Results showed that the WSI S-scheme heterojuction has a synergistic effect for the removal of BPA and Cr(VI). An optimum case of the WSI-12% heterojunction exhibited the highest photocatalytic efficiency in the degradation of BPA under visible light, which is ca. 2.5 and 3.8 times more than the pure WO3 and SIS, respectively. The enhanced photocatalytic activity is attributed to the formation of the WSI S-scheme heterojunctions which facilitate the spatial separation of charge carriers and preserve strong photoredox ability. Further, the S-scheme mechanism of enhanced photocatalysis was examined by the radical-trapping experiment and ESR, and superoxide and hydroxyl radicals were determined to be the major reactive oxygen species responsible for BPA degradation and Cr(VI) reduction by WSI. This work provides a novel strategy for tailoring high-performance S-scheme heterojunctions and shows the promising application in purifying wastewater with complex pollutants.
The Posttraumatic Increase of the Adhesion GPCR EMR2/ADGRE2 on Circulating Neutrophils Is Not Related to Injury Severity
Authors: Zheng Leyu , Rang Moujie , Fuchs Carolin , Keß Annette , Wunsch Mandy , Hentschel Julia , Hsiao Cheng-Chih , Kleber Christian , Osterhoff Georg , Aust Gabriela
Published: November 2023

Trauma triggers a rapid innate immune response to aid the clearance of damaged/necrotic cells and their released damage-associated molecular pattern (DAMP). Here, we monitored the expression of EMR2/ADGRE2, involved in the functional regulation of innate immune cells, on circulating neutrophils in very severely and moderately/severely injured patients up to 240 h after trauma. Notably, neutrophilic EMR2 showed a uniform, injury severity- and type of injury-independent posttraumatic course in all patients. The percentage of EMR2+ neutrophils and their EMR2 level increased and peaked 48 h after trauma. Afterwards, they declined and normalized in some, but not all, patients. Circulating EMR2+ compared to EMR2− neutrophils express less CD62L and more CD11c, a sign of activation. Neutrophilic EMR2 regulation was verified in vitro. Remarkably, it increased, depending on extracellular calcium, in controls as well. Cytokines, enhanced in patients immediately after trauma, and sera of patients did not further affect this neutrophilic EMR2 increase, whereas apoptosis induction disrupted it. Likely the damaged/necrotic cells/DAMPs, unavoidable during neutrophil culture, stimulate the neutrophilic EMR2 increase. In summary, the rapidly increased absolute number of neutrophils, especially present in very severely injured patients, together with upregulated neutrophilic EMR2, may expand our in vivo capacity to react to and finally clear damaged/necrotic cells/DAMPs after trauma.
Neuroprotective Action of Tacrolimus before and after Onset of Neonatal Hypoxic–Ischaemic Brain Injury in Rats
Authors: Smith Madeleine J. , Penny Tayla , Pham Yen , Sutherland Amy E. , Jenkin Graham , Fahey Michael C. , Paton Madison C. B. , Finch-Edmondson Megan , Miller Suzanne L. , McDonald Courtney A.
Published: November 2023

(1) Background: Neonatal brain injury can lead to permanent neurodevelopmental impairments. Notably, suppressing inflammatory pathways may reduce damage. To determine the role of neuroinflammation in the progression of neonatal brain injury, we investigated the effect of treating neonatal rat pups with the immunosuppressant tacrolimus at two time points: before and after hypoxic–ischaemic (HI)-induced injury. (2) Methods: To induce HI injury, postnatal day (PND) 10 rat pups underwent single carotid artery ligation followed by hypoxia (8% oxygen, 90 min). Pups received daily tacrolimus (or a vehicle) starting either 3 days before HI on PND 7 (pre-HI), or 12 h after HI (post-HI). Four doses were tested: 0.025, 0.05, 0.1 or 0.25 mg/kg/day. Pups were euthanised at PND 17 or PND 50. (3) Results: All tacrolimus doses administered pre-HI significantly reduced brain infarct size and neuronal loss, increased the number of resting microglia and reduced cellular apoptosis (p < 0.05 compared to control). In contrast, only the highest dose of tacrolimus administered post-HI (0.25 mg/kg/day) reduced brain infarct size (p < 0.05). All doses of tacrolimus reduced pup weight compared to the controls. (4) Conclusions: Tacrolimus administration 3 days pre-HI was neuroprotective, likely mediated through neuroinflammatory and cell death pathways. Tacrolimus post-HI may have limited capacity to reduce brain injury, with higher doses increasing rat pup mortality. This work highlights the benefits of targeting neuroinflammation during the acute injurious period. More specific targeting of neuroinflammation, e.g., via T-cells, warrants further investigation.
Liquid Biopsy in Head and Neck Cancer: Its Present State and Future Role in Africa
Authors: Temilola Dada Oluwaseyi , Adeola Henry Ademola , Grobbelaar Johan , Chetty Manogari
Published: November 2023

The rising mortality and morbidity rate of head and neck cancer (HNC) in Africa has been attributed to factors such as the poor state of health infrastructures, genetics, and late presentation resulting in the delayed diagnosis of these tumors. If well harnessed, emerging molecular and omics diagnostic technologies such as liquid biopsy can potentially play a major role in optimizing the management of HNC in Africa. However, to successfully apply liquid biopsy technology in the management of HNC in Africa, factors such as genetic, socioeconomic, environmental, and cultural acceptability of the technology must be given due consideration. This review outlines the role of circulating molecules such as tumor cells, tumor DNA, tumor RNA, proteins, and exosomes, in liquid biopsy technology for the management of HNC with a focus on studies conducted in Africa. The present state and the potential opportunities for the future use of liquid biopsy technology in the effective management of HNC in resource-limited settings such as Africa is further discussed.
Chromophore-Targeting Precision Antimicrobial Phototherapy
Authors: Jusuf Sebastian , Dong Pu-Ting
Published: November 2023

Phototherapy, encompassing the utilization of both natural and artificial light, has emerged as a dependable and non-invasive strategy for addressing a diverse range of illnesses, diseases, and infections. This therapeutic approach, primarily known for its efficacy in treating skin infections, such as herpes and acne lesions, involves the synergistic use of specific light wavelengths and photosensitizers, like methylene blue. Photodynamic therapy, as it is termed, relies on the generation of antimicrobial reactive oxygen species (ROS) through the interaction between light and externally applied photosensitizers. Recent research, however, has highlighted the intrinsic antimicrobial properties of light itself, marking a paradigm shift in focus from exogenous agents to the inherent photosensitivity of molecules found naturally within pathogens. Chemical analyses have identified specific organic molecular structures and systems, including protoporphyrins and conjugated C=C bonds, as pivotal components in molecular photosensitivity. Given the prevalence of these systems in organic life forms, there is an urgent need to investigate the potential impact of phototherapy on individual molecules expressed within pathogens and discern their contributions to the antimicrobial effects of light. This review delves into the recently unveiled key molecular targets of phototherapy, offering insights into their potential downstream implications and therapeutic applications. By shedding light on these fundamental molecular mechanisms, we aim to advance our understanding of phototherapy’s broader therapeutic potential and contribute to the development of innovative treatments for a wide array of microbial infections and diseases.
Quantitative Analysis of Meteorite Elements Based on the Multidimensional Scaling–Back Propagation Neural Network Algorithm Combined with Raman Mapping-Assisted Micro-Laser Induced Breakdown Spectroscopy
Authors: Wang Hongpeng , Xin Yingjian , Fang Peipei , Wang Yian , Duan Mingkang , Wu Wenming , Yang Ruidong , Liu Sicong , Zhang Liang , Wan Xiong
Published: November 2023

Meteorites are an essential reference for human exploration of the universe and its cosmic evolution and an essential research object for searching for extraterrestrial life. Ways to quickly identify and screen suspected meteorite samples have become the foundation and prerequisite for research on high-value meteorite samples. Therefore, this paper proposes a Raman mapping-assisted micro-laser induced breakdown spectroscopy (micro-LIBS) technology for field detection of suspected meteorite material composition without sample pre-processing, with a high detection speed and cost-effectiveness, to realize the detection of element composition and molecular structure. Raman mapping carries out multispectral imaging with high spectral resolution of the region of interest. The fusion of Raman mapping and optical microscopy images can provide mineral categories and spatial distribution characteristics in regions of interest. A quantitative analysis model for Fe, Mg, and Na elements was constructed based on the multidimensional scaling–back propagation neural network (MDS-BPNN) algorithm. The determination coefficient of the model test set was better than 0.997, and the root mean square error was better than 0.65. The content of Fe, Mg, and Na elements in the meteorite was preliminarily evaluated, providing a reference for further analysis of element information in spectral image fusion data. The Raman–LIBS combined technology has significant application potential in rapidly evaluating suspected meteorite samples. Without high-end precision instruments or field research, this technology can provide scientists with significant reference value atomic and molecular spectral information. At the same time, this technology can be extended to other petrology research. We offer a fast, efficient, cost-effective, and reliable analysis scheme for reference.
Detection of Xylene Using Ni(OH)2-Enhanced Co3O4 Nanoplate via p–n Junctions
Authors: Ran Mengran , Yuan Zhenyu , Zhu Hongmin , Gao Hongliang , Meng Fanli
Published: November 2023

This study reports a novel Ni(OH)2/Co3O4 heterostructured nanomaterial synthesized through a simple two-step hydrothermal method combined with subsequent heat treatment. The Ni(OH)2/Co3O4 heterostructured nanomaterial showed excellent performance in the detection of xylene gas. XRD, SEM, and EDS characterized the crystal structure, microstructure, and composition elements of Co3O4 and Ni(OH)2/Co3O4, and the gas sensing properties of the Co3O4 sensor and Ni(OH)2/Co3O4 sensor were systematically tested. The test results indicate the Ni(OH)2/Co3O4 sensor has an optimal operating temperature of 175 °C, which is 10 °C lower than that of the Co3O4 sensor; has a response of 14.1 to 100 ppm xylene, which is 7-fold higher than that of the Co3O4 sensor; reduces the detection limit of xylene from 2 ppm to 100 ppb; and has at least a 4-fold higher response to xylene than other gases. The Ni(OH)2/Co3O4 nanocomposite exerts the excellent catalytic performance of two-dimensional nanomaterial Ni(OH)2, solves the deficiency in the electrical conductivity of Ni(OH)2 materials, and realizes the outstanding sensing performance of xylene, while the construction of the p–n heterojunction between Ni(OH)2 and Co3O4 also improves the sensing performance of the material. This study provides a strategy for designing high-performance xylene gas sensors using two-dimensional Ni(OH)2 materials.