bluuebunny/crossref_metadata_2025 huggingface dataset:
Rab35 Is Required for Embryonic Development and Kidney and Ureter Homeostasis through Regulation of Epithelial Cell Junctions
Authors: Clearman Kelsey R. , Timpratoom Napassawon , Patel Dharti , Rains Addison B. , Haycraft Courtney J. , Croyle Mandy J. , Reiter Jeremy F. , Yoder Bradley K.
Published: March 2024

Key Points

Loss of Rab35 leads to nonobstructive hydronephrosis because of loss of ureter epithelium.

Rab35 regulates kidney and ureter epithelial cell adhesion and polarity.

Rab35 is required for embryonic development.

Background

Rab35 is a member of a GTPase family of endocytic trafficking proteins. Studies in cell lines have indicated that Rab35 participates in cell adhesion, polarity, cytokinesis, and primary cilia length and composition. In addition, sea urchin Rab35 regulates actin organization and is required for gastrulation. In mice, loss of Rab35 in the central nervous system disrupts hippocampal development and neuronal organization. Outside of the central nervous system, the functions of mammalian Rab35 in vivo are unknown.

Methods

We generated and analyzed the consequences of both congenital and conditional null Rab35 mutations in mice. Using a LacZ reporter allele, we assessed Rab35 expression during development and postnatally. We assessed Rab35 loss in the kidney and ureter using histology, immunofluorescence microscopy, and western blotting.

Results

Congenital Rab35 loss of function caused embryonic lethality: homozygous mutants arrested at E7.5 with cardiac edema. Conditional loss of Rab35, either during gestation or postnatally, caused hydronephrosis. The kidney and ureter phenotype were associated with disrupted actin cytoskeletal architecture, altered Arf6 epithelial polarity, reduced adherens junctions, loss of tight junction formation, defects in epithelial growth factor receptor expression and localization, disrupted cell differentiation, and shortened primary cilia.

Conclusions

Rab35 may be essential for mammalian development and the maintenance of kidney and ureter architecture. Loss of Rab35 leads to nonobstructive hydronephrosis, making the Rab35 mutant mouse a novel mammalian model to study mechanisms underlying this disease.
Electrical status epilepticus “invisible” to surface EEG in late‐onset Rasmussen encephalitis
Authors: Fattouch Jinane , Di Bonaventura Carlo , Di Gennaro Giancarlo , Quarato Pier Paolo , Petrucci Simona , Manfredi Mario , Prencipe Massimilano , Esposito Vincenzo , Giallonardo Anna Teresa
Published: March 2024

Rasmussen's encephalitis (RE) is a chronic, progressive disease that typically occurs in childhood, rarely in adulthood. When it does occur in adulthood, it may be associated with atypical electro‐clinical features, and neuroimaging alterations usually help diagnosis. Unlike childhood forms, in which epilepsia partialis continua is usually observed, the EEG pattern in adult variants may be aspecific. We describe a highly interesting case of late‐onset RE in which an electrical status epilepticus was not detected by the surface EEG, but by a recording with subdural electrodes.
Clinical, semiological, electroencephalographic, and neuropsychological features of “pure” neocortical temporal lobe epilepsy
Authors: Maizuliana Hana , Usui Naotaka , Terada Kiyohito , Kondo Akihiko , Inoue Yushi
Published: March 2024

Aims
: We examined the clinical, semiological, scalp EEG, and neuropsychological features of patients with “pure” neocortical temporal lobe epilepsy (NTLE) who were successfully treated by neocortical temporal resection sparing the mesial temporal structures.

Methods
: This retrospective study included 17 patients with lesional NTLE who satisfied the following criteria: presence of a discrete structural lesion in the lateral temporal lobe on preoperative MRI; lateral temporal resection sparing the mesial temporal structures; follow‐up for at least two years after surgery; and favourable postoperative seizure outcome (Engel Class I). The study included 10 females and seven males, and the age at surgery ranged from 15 to 48 years (mean: 30.7 years). Auras, video‐recorded seizure semiology, interictal and ictal EEG, and pre‐ and post‐operative neuropsychological data were reviewed. Twenty patients with mesial temporal lobe epilepsy (MTLE) with hippocampal sclerosis, who had a favourable postoperative seizure outcome (Engel Class I), were selected as a control group.

Results
: Age at seizure onset was significantly greater in patients with NTLE than in controls. A history of febrile convulsion was significantly less frequent in NTLE patients. Epigastric ascending sensation (6%
versus
40%;
p
=0.017), oral automatisms (29%
versus
80%;
p
=0.003), gestural automatisms (47%
versus
80%;
p
=0.047), and dystonic posturing (0%
versus
40%;
p
=0.003) were significantly less frequent in NTLE than controls. Ictal unitemporal rhythmic theta activity was also significantly less frequent in NTLE than controls (35.3%
versus
75%;
p
=0.015). Preoperative IQ score (range: 68 to 114; mean: 88.9) and preoperative memory quotient score (range: 56–122; mean: 98.1) were significantly higher in NTLE (
p
=0.003 and
p
=0.048, respectively).

Conclusion
: There were notable differences in clinical, semiological, EEG, and neuropsychological features between “pure” NTLE and MTLE. These findings may be useful to identify the epileptogenic zone.
Startle response: epileptic or non‐epileptic? The case for “flash” SMA reflex seizures
Authors: Cokar Özlem , Gelisse Philippe , Livet Marie‐Odile , Bureau Michelle , Habib Michel , Genton Pierre
Published: March 2024

A 19‐year‐old woman complained of long‐standing, frequent, debilatating brusque movements triggered by unexpected stimuli. She was neurologically normal and neuroimaging was also normal. Conspicuous startle reactions were easily reproduced under EEG and video monitoring: the interictal EEG was normal, the ictal recordings doubtful; clinically, the startle reaction was asymmetric, with elevation of the left limbs. The diagnosis of hyperekplexia and startle epilepsy were discussed. We learned that she had been evaluated at age 3‐4 months for spontaneous, generalised tonic‐clonic seizures and “infantile spasms”, in fact for early‐onset startle reactions triggered by noise or contact, in association with prominent EEG changes. A full remission had been achieved under ACTH therapy, but the startle reactions had reappeared at around age six. The patient was successfully treated with carbamazepine. The history, clinical and neurophysiological data led us to discuss the diagnosis of hyperekplexia and startle epilepsy. We concluded that the patient had an unusual form of cryptogenic focal epilepsy originating from the supplementary motor area, presenting as strictly stimulus‐triggered “flash” seizures.
Epilepsy and neuroprotection: an illustrated review
Authors: Arzimanoglou Alexis , Hirsch Edouard , Nehlig Astrid , Castelnau Pierre , Gressens Pierre , de Vasconcelos Anne Pereira
Published: March 2024

Multiple types of insults, such as status epilepticus, hypoxia and trauma, may alter the central nervous system. Strategies to protect the brain against insults remain a very difficult and challenging problem. Damage to the central nervous system can be modulated via excessive excitatory and reduced inhibitory neurotransmission. In addition, increased sodium and calcium loading through impaired voltage‐sensitive channels, as well as alterations in the acid‐base balance can contribute to both excitotoxic and apoptotic cell death.

Epilepsy treatment has always been related to neuroprotection, since it aims to reduce the duration or totally suppress seizures. Although the debate on the capacity of simple seizures to induce neuronal injury is still ongoing, no doubt persists on the disastrous effects of prolonged episodes of status. The next step would be to prevent epilepsy.

Several animal models have been used to study the various aspects of the epileptogenic process. In humans, one of the most compelling examples of a series of epileptogenic events is temporal lobe epilepsy (TLE). Temporal lobe epilepsy is often attributed to prolonged febrile convulsions in childhood resulting in mesial temporal sclerosis. However, the relationship between TLE, seizures in childhood and hippocampal sclerosis may not be apparent as initially believed. Furthermore, it is well recognized that in a number of patients there is a delay from a specific insult to the onset of seizures. This “latent period” could be an opportunity for effective intervention, provided that the underlying mechanisms are understood and that appropriate means for a beneficial modification of the disease process become available.

The present review discusses the various steps of temporal lobe epilepsy and provides illustrations of the various mechanisms implicated in neuronal death. Data from animal models is also presented and illustrated with video sequences. Finally, on the basis of what is known on mechanisms of action of available antiepileptic drugs, some suggestions are put forward.

Basic science and research are guided by clinical queries and from ongoing dialogue. The present illustrated review deals with only a small part of the important amount of work related to epilepsy and neuroprotection. As such it is necessarily schematic or even simplistic. The review is designed to inform clinicians about the basic issues related to the subject, thus allowing them to follow the ongoing debate and participate with pertinent questions. (Published with video sequences).
Recent developments in treatment of status epilepticus: A review
Authors: Rosenow Felix , Arzimanoglou Alexis , Baulac Michel
Published: March 2024

Considering that status epilepticus (SE) is a medical emergency associated with significant morbidity and mortality, there are surprisingly few evidence‐based data to guide management decisions. The purpose of this review is to give an overview of the incidence and classification of SE and to summarise the recent developments in the treatment of generalized tonic clonic status epilepticus (GTCSE). These consist in two prospective randomised studies indicating that SE should be treated as soon as possible, even out‐of‐hospital, by intravenous (IV) benzodiazepine [1, 2]. Lorazepam is probably the best choice for the initial therapy. However, the differences in efficacy as compared to diazepam [1, 3], diazepam associated to phenytoin or phenobarbital [2] were not significant. There is no Class I evidence to help us choose which drug to give in SE that is not responsive to the initial lorazepam. Traditionally, based on a long clinical experience, IV phenytoin is given as the second drug. Recently, phenytoin is being increasingly substituted by fosphenytoin, even though no formal, comparative tolerability studies have been performed to study this compound in GTCSE. Starting in the 1980's, the use of injectable valproic acid (IV VPA) has been reported in an increasing number of uncontrolled case series initiated by doctors, indicating relative easy use, relative good tolerability and suggesting that it may be efficacious. Finally, we have very little data concerning the treatment of SE refractory to a benzodiazepine and phenytoin. Despite this lack of data many centres today use midazolam or propofol rather than phenobarbital or pentobarbital in this setting because these compounds have short half‐lives and are, therefore, easier to handle.
Pilomotor seizures and status in non‐paraneoplastic limbic encephalitis
Authors: Wieser Stephan , Kelemen Anna , Barsi Peter , Vincent Angela , Borbely Csaba , Rasonyi Gyorgy , Mueller Susanne , Hess Klaus , Wieser Heinz Gregor , Halasz Peter
Published: March 2024

Background and aims

To describe an unusual clinical presentation of a patient with voltage‐gated potassium channel Ab‐ positive, nonparaneoplastic limbic encephalitis.

Methods

We performed video‐EEG monitoring, structural MRI,
18
F‐FDG‐PET,
1
H‐MRS, neuropsychological testing and antibody serology.

Results

A 42‐year‐old male patient presented in an acute phase of non‐paraneoplastic limbic encephalitis confirmed by MRI, with antibodies to voltage‐gated potassium channels. His pilomotor status was pharmacoresistant to antiepileptic drugs, but responded to corticosteroid and azathioprine treatment. The MRI findings improved. The pilomotor seizures recurred when the immunosuppressive therapy was discontinued after 18 months. MRI at that time was consistent with hippocampal sclerosis. Complete seizure control was achieved after reintroduction of steroids.

Conclusion

Pilomotor seizures were the predominant seizure type in this case of non‐paraneoplastic limbic encephalitis. Immunosuppressive therapy may provide recovery including seizure control. However, long‐term immunosuppression may be necessary to prevent relapse. Hippocampal sclerosis and chronic epilepsy might evolve as sequelae of limbic encephalitis.
‘Mae popeth yn troi o amgylch yr arholiad’: Beth yw canfyddiadau a phrofiadau myfyrwyr ysgol uwchradd o ddysgu Cymraeg fel ail iaith yng Nghymru?
Authors: Rhys Mirain , Smith Kevin
Published: March 2024

Yn 2017, cyflwynodd Llywodraeth Cymru Cymraeg 2050, strategaeth uchelgeisiol i gynyddu nifer y siaradwyr Cymraeg yng Nghymru (Llywodraeth Cymru, 2017). Mae system addysg Cymru yn nodwedd hanfodol bwysig o’r strategaeth hon. Yn 2013, nododd adolygiad o addysgu Cymraeg yng Nghyfnodau Allweddol 3 a 4 (Davies, 2013) fylchau sylweddol yn y gwaith o baratoi a hyfforddi athrawon i addysgu Cymraeg fel Ail Iaith, ethos Cymraeg gwael mewn llawer o ysgolion, a diffyg cyffredinol o ran adnoddau i gefnogi addysgu’r Gymraeg. Roedd yr argymhellion yn cynnwys sawl dimensiwn gan gynnwys cynnwys y cwricwlwm, ymarfer addysgegol, ac asesu, hyfforddiant ac adnoddau athrawon. Gyda dyfodiad fframwaith cwricwlaidd cenedlaethol newydd (Llywodraeth Cymru, 2015), mae’r astudiaeth hon yn adeiladu ar adolygiad Davies (2013) ac yn rhoi manylion digynsail am ganfyddiadau myfyrwyr o addysgu Cymraeg. Dadansoddwyd data a gynhyrchwyd drwy grwpiau ffocws gyda myfyrwyr gan ystyried damcaniaeth dynameg gr?p Dörnyei, gan roi beirniadaeth ddeifiol o’r nodau ar gyfer addysgu Cymraeg, ymarfer addysgeg athrawon, a’r hyn y mae myfyrwyr yn awgrymu ei fod yn ddiffyg cysylltiad cyffredinol rhwng addysgu Cymraeg a’r defnydd o’r iaith yn eu bywydau bob dydd.
Colli cynefin – colli ein lle, colli ein cartref, colli ein hunan
Authors: Adams Dylan , Beauchamp Gary
Published: March 2024

Mae tystiolaeth lethol yn awgrymu ein bod yn wynebu argyfwng hinsawdd ar hyn o bryd oherwydd effeithiau dynol ar brosesau planedol hanfodol. Ar yr un pryd, mae Cymru wrthi’n gwneud diwygiadau arwyddocaol i’r cwricwlwm. Fel rhan o’r diwygiadau, mae’r gair cynefin yn ymddangos yng nghanllawiau Cwricwlwm i Gymru. Mae’r papur hwn yn dadansoddi sut mae gorbwyslais ar safbwyntiau epistemolegol ac ontolegol cyfyngedig ym maes addysg wedi helpu i greu darlun tlodaidd o’r hunan sydd wedi gwaethygu ein perthynas afiach â natur. Cynigir y gellid defnyddio’r gair cynefin yn gysyniadol i awgrymu cyflyrau amgen o fod a ffyrdd o wybod sy’n cynnwys gwell ymdeimlad o’r hunan. Awgrymir y gall plant ymgysylltu â’r byd naturiol drwy safbwyntiau ontolegol cryfach wrth archwilio ffyrdd o wybod sy’n cael eu gwthio i’r cyrion yn yr ystafell ddosbarth brif ffrwd fel arfer. Mae gwir angen am hyn gan y gallai creu cyfleoedd i blant ddod i gytgord â’u cyfranogiad cydgysylltiedig â’r byd mwy na dynol feithrin perthynas adnewyddol ac adferol â natur. At hynny, gallai alluogi’r profiad o ddealltwriaeth ddirfodol ehangach.
Rhagweld cyfraddau dysgu myfyrwyr israddedig mewn darlith: Rôl hunanreolaeth, cymhelliant, ac ymdrech feddyliol
Authors: Owen Robin , Blanchfield Anthony , Gottwald Vicky
Published: March 2024

Am y tro cyntaf, ymchwiliodd yr astudiaeth bresennol i rôl gyfunol hunanreolaeth, cymhelliant ac ymdrech feddyliol wrth ragweld dysgu myfyrwyr israddedig o ddarlith addysg uwch ar ffurf cyflwyniad dwyawr. Roedd yr astudiaeth yn cynnwys 62 o fyfyrwyr, mewn darlith Hyfforddi Chwaraeon blwyddyn olaf BSc israddedig, a gwblhaodd holiaduron yn mesur: hunanreolaeth cyflwr yn ystod y ddarlith; dull meistrolaeth a chymhelliant osgoi perfformiad tuag at ddysgu pwnc y cwrs; ymdrech feddyliol a wnaed yn ystod y ddarlith; a’r gallu i gofio cynnwys yn syth ar ôl y ddarlith (h.y., dysgu). Datgelodd dadansoddiadau cyfryngu wedi’u cymedroli fod angen lefelau uwch o hunanreolaeth cyflwr (newidyn W) er mwyn i fyfyrwyr drawsffurfio eu cymhelliant (newidyn X; dull meistrolaeth neu osgoi perfformiad) i ymdrech feddyliol (newidyn M) er budd eu dysgu (newidyn Y) yn ystod y ddarlith. Trafodir llwybrau ar gyfer ymyriadau cymhwysol i ysgogi myfyrwyr a chynyddu eu hadnoddau hunanreolaeth mewn amgylcheddau addysg uwch.
La distribución semántica de los constituyentes periféricos de izquierda y de derecha en hablantes de español L1, sueco L1 y español L2
Authors: Fant Lars , Österberg Rakel
Published: March 2024

This paper investigates the structure of syntactic peripheries in the colloquial speech produced by three groups: native users of Spanish, native users of Swedish, and Swedish very advanced users of L2 Spanish. The main aspect addressed is the distribution of left- and right-periphery constituents across three categories defined in semantic terms, viz. denotative, indexical and non-referential (pragmatic) expressions. Our research questions concern (1) to what extent two typologically distinct languages such as Spanish and Swedish differ in this respect, (2) to what extent the Spanish L2 productions align with native use, and (3) whether any transfer from Swedish occurs in the Spanish L2 formulations. The analysis shows important divergences between Spanish and Swedish native use, not only with regard to the size of the respective peripheries but also in the distribution across the three semantic categories. Thus, although in all three groups the proportion of pragmatic constituents is much larger than for the two other categories, this gap is considerably wider in the Swedish L1 data. As regards the Spanish L2 data, a high degree of alignment with native use can be reported, and hardly any transfer from Swedish can be found in these highly proficient L2 speakers. However, a certain overrepresentation of indexical expressions can be observed in the L2 data.
El léxico de las emociones y el discurso fraseológico. Aspectos cognitivos y uso en hablantes con enfermedad de Alzheimer
Authors: Penas Ibáñez María Azucena , Ivanova Olga
Published: March 2024

Adopting a cognitive approach, this paper addresses two questions: the lexical-semantic analysis of emotional phraseology in normotypical speakers of Spanish and the study of the function and use of emotional phraseology in speakers with semantic deficits, specifically, in speakers with Alzheimer’s disease (AD). The paper pursues two objectives: to provide a brief state of the art on the somatic verbalization of emotions through phraseological units and to study the presence and function of such phraseological units in speakers with neurodegenerative diseases, in particular, AD.
Occurrence of selected pharmaceuticals in wastewater and sludge samples from wastewater treatment plants in Eastern Cape province of South Africa
Authors: Netshithothole R , Managa M , Botha T , Madikizela L
Published: March 2024

ABSTRACT The occurrence of pharmaceuticals in various wastewater treatment plants (WWTPs) and their discharge into the surface water are existing global challenges. However, such challenges are more detrimental in developing countries due to the aging infrastructure and its vandalism influenced by poverty, resulting in the illegal breakdown of the WWTPs. This study investigated the presence of efavirenz, ibuprofen, naproxen, sulfamethoxazole, and trimethoprim in WWTPs. Ultrasound-assisted extraction (UAE) followed by solid-phase extraction (SPE) was used to extract these pharmaceuticals in sludge samples, with wastewater extracted with only the latter. This was followed with analysis using a high-performance liquid chromatography-photo-diode array detection system. Recoveries found after spiking the samples with analytes at different concentrations ranged from 56 to 117%. An antibiotic, sulfamethoxazole, was among the prominent drugs in untreated wastewater with its concentration reaching 77 ug L-1. All the analytes were detected in sludge samples, with naproxen having the highest concentration of 13.35 ng g-1. The pharmaceutical with the lowest removal efficiency (2-12%) in WWTPs was efavirenz, while other drugs were fairly removed from wastewater. Overall, the findings of this study indicate the dysfunctionality of selected WWTPs in the Eastern Cape province of South Africa due to the release of high amounts of pharmaceuticals into the surface water which can be detrimental to humans, animals, and aquatic life.
Análisis de la multidimensionalidad del brand equity para el sector bancario: un estudio en la generación Z
Authors: López-Rodríguez Campo Elías , Sotelo-Muñoz Johanna Katherine , Muñoz-Venegas Ingrid Janeth , López-Aguas Nicolás Fabián
Published: March 2024

en el escenario competitivo del sector bancario, las actividades de marketing y branding se han convertido en un elemento crucialpara el éxito a largo plazo de las instituciones financieras. Desde la gestión del brand equity, la confianza y la lealtad son esenciales, no solo enlos procesos operativos con los usuarios, sino en la consolidación de la marca como un activo estratégico. El objetivo de esta investigación fueanalizar la multidimensionalidad del brand equity para el sector bancario desde la perspectiva de la generación Z. Se desarrolla un estudio de tipocuantitativo, analítico y de corte transversal, utilizando el análisis factorial exploratorio y confirmatorio a partir de un instrumento aplicado deforma on line a personas pertenecientes a este segmento generacional. Los hallazgos infieren que el brand equity en el sector bancario dirigido ala generación Z está conformado por las siguientes dimensiones: fidelidad e importancia de la marca (FIM), calidad percibida (CP), asociación demarca (AM), rendimiento de la marca (RM) y conciencia de la marca (CM). Se concluye que el sector bancario debe seguir consolidando su brandequity como actividad estratégica a partir de dichas dimensiones, fortaleciendo el posicionamiento de sus marcas, su participación y su orientaciónal mercado, garantizando el entendimiento de las necesidades, deseos y comportamientos de la generación Z.
Predicción del comportamiento de compra online: una aplicación del modelo S-O-R
Authors: Csoban-Mirka Eugenia , Esqueda Henríquez Sofía , Ríos Alfredo
Published: March 2024

El objetivo de este estudio es evaluar el efecto de la atmósfera, definida como el diseño consciente del ambiente de la tienda para crearciertas respuestas en los compradores, en los valores de compra, la actitud hacia la compra y las emociones sobre la intención de recompra en unatienda online, basado en el modelo Estímulo-Organismo-Respuesta (S-O-R, por sus siglas en inglés). Aunque estos elementos han sido estudiadosampliamente en el contexto de tiendas físicas, artículos recientes que analizan la influencia de estos elementos en el ambiente online muestran larelevancia de estudios como este en investigaciones académicas actuales de mercadeo. Los resultados de una encuesta realizada a 306 usuarios de unatienda farmacéutica online, residentes de Venezuela y otros países de América Latina, se analizaron usando ecuaciones estructurales. Los hallazgospermiten concluir que el uso del modelo S-O-R es apropiado en la predicción del comportamiento de compra online, y confirman que el valor decompra hedónico tiene un impacto directo significativo tanto en la actitud hacia las compras como en la compra emocional. Por otra parte, el valor decompra utilitario afecta negativamente la compra emocional y el factor informático favorece la actitud hacia las compras. Por último, la actitud hacia las compras y la compra emocional predicen la intención de recompra. Se discuten las implicaciones para los especialistas en marketing.
Le domaine familial comme lieu d’apprentissage des langues et de l’imaginaire chez Vladimir Nabokov
Authors: GASSIN Alexia
Published: March 2024

Dès son plus jeune âge, Vladimir Nabokov se voit immergé dans trois cultures européennes distinctes, inculquées par ses parents, gouvernantes et précepteurs, grâce à qui il apprend également les langues correspondantes : le russe, l’anglais et le français. Ces faits sont non seulement relatés dans son autobiographie, intitulée Autres rivages (1951), mais aussi dans ses romans et nouvelles, tels que L’Exploit (1932) ou Mademoiselle O (1936), composés au cours de sa période russe et dans lesquels l’écrivain attribue à ses protagonistes certains événements vécus par son propre « je » plurilingue. Il s’agira ici de porter une attention particulière aux deux grands foyers russes de l’auteur où le jeune Nabokov a passé toute son enfance et son adolescence et a pu développer son trilinguisme.
Enfants translingues. Le bleu des abeilles de Laura Alcoba et Marx et la poupée de Maryam Madjidi
Authors: De BALSI Sara
Published: March 2024

Cette contribution analyse deux romans autobiographiques qui rendent compte de la rencontre avec la langue française et de l’épreuve qu’a constituée son apprentissage : Le bleu des abeilles de Laura Alcoba (2013) et Marx et la poupée de Maryam Madjidi (2017). Les deux autrices ont en commun d’être arrivées en France pendant leur enfance, à l’âge de dix ans pour l’une et de six ans pour l’autre, pour suivre un parent dans son exil politique, respectivement d’Argentine et d’Iran. La France et la langue française ne sont donc pas choisies, mais, au moins dans un premier temps, subies. Nous examinons comment ces deux textes représentent la formation de la conscience linguistique de l’enfant dans le pays natal, la rupture que constituent l’exil et la rencontre avec la langue étrangère, et enfin la formation d’une identité translingue, consciente d’un « avant » et d’un « après » et d’un « là-bas » et d’un « ici », deux espace-temps qu’il ne peut jamais habiter simultanément. Nous abordons aussi l’imaginaire de la langue natale et seconde qui en ressort et les stratégies de leur mise en scène textuelle.
Ambivalence du récit de soi, tentation romanesque. La langue oubliée d’Emmanuel Carrère
Authors: AUBRY-MORICI Marine
Published: March 2024

Né d’une mère russe et spécialiste universitaire de l’URSS, l’enfant bilingue Emmanuel Carrère a ou-blié ce qui fut la langue de son enfance (« j’ai parlé russe enfant »). Deux de ses récits autobiogra-phiques Un roman russe (POL, 2007) et Limonov (POL, 2011), contraignent néanmoins le narrateur-enquêteur à reprendre l’apprentissage de cette langue pour mener à bien ses recherches en Russie. L’écrivain oscille alors entre le plaisir des sonorités retrouvées et la douleur des échecs à se faire com-prendre dans un « russe de cuisine ». Servant idéalement sa réflexion sur l’identité, le double et l’insaisissabilité d’une personnalité, y compris la sienne, le bilinguisme est un thème mineur qui s’avère révélateur de grandes lignes de force de l’œuvre. Est-il possible de rattacher cette dynamique, celle d’une perte de contrôle à la fois crainte et recherchée, à la tentation romanesque à l’œuvre dans la non-fiction de Carrère ?
Un je(u) d’enfant. Ritournelle ritale et création littéraire chez François Cavanna
Authors: RUSSO Ugo
Published: March 2024

Dans son premier livre autobiographique, Les Ritals (1978), François Cavanna raconte son enfance et rend hommage à son père Luigi, maçon immigré italien. Cavanna fils s’applique à retranscrire fidèlement la langue paternelle, ses approximations lexicales, syntactiques et phonétiques ; ses intensités carnavalesques également, ainsi que le plaisir jubilatoire qu’elle véhicule. Cette langue mineure, hybride, anti-littéraire, devient ainsi le pré-texte de la création littéraire : la ritournelle ritale de Cavanna père devient le motif et le moteur de l’écriture du fils.
La rhétorique de la « marche du silence » au Mexique en 1968
Authors: EGGLER Marcel
Published: March 2024

Dès le 22 juillet 1968, la ville de Mexico a été le témoin d’une escalade du conflit entre un gouvernement autoritaire et des milliers d’étudiants protestataires qui, comme dans de nombreuses autres régions du monde, réclamaient l’introduction d’un système démocratique, anarchiste ou socialiste (Rojas 2018). Le conflit s’est terminé le 2 octobre 1968 par un bain de sang sur la Plaza de las Tres Culturas : « le massacre de Tlatelolco ». Dans cet article, seront examinées, d’une part, l’escalade politico-communicative qui a précédé le massacre, et, d’autre part, la « marche du silence » du 13 septembre 1968 qui a conduit à la réponse violente des forces répressives du gouvernement de Díaz Ordaz. Le silence ou l’arrêt soudain de la communication (aposiopèse) est considéré comme un procédé rhétorique depuis l’Antiquité. Plus de 200 000 manifestants choisirent ce mode de communication pour réclamer plus de démocratie. Cet article examine les mécanismes de l’escalade de la violence, la force de ce silence collectif, et la manière dont il échoua face à la violence organisée d’un gouvernement autoritaire.
Short report on the excavations of the Legionary Bath of Brigetio in 2023
Authors: Bartus Dávid , Szabó Melinda , Juhász Lajos , Müller Ákos , Olasz Rita Helga , Simon Bence , Borhy László , Számadó Emese
Published: March 2024

Since 2015, excavations have been carried out in different parts of the legionary fortress in Brigetio. Due to the systematic geophysical surveys of the praetentura, a large building complex came to light, which could be identified as the bath of the fortress, even before the fieldworks started in the territory. In the area of this building complex, the excavations started in 2021, and continued in the next two years. Now, an approximately 1800 m2 surface of the bath is excavated. During the last season of the fieldwork several heated and unheated rooms and three new (possible) pools and four praefurnia were unearthed. The north-eastern closure of the bath was identified, and parts of the northern apses of the basilica thermarum were unearthed. A huge number of coins and bronze small finds were discovered, and thanks to the brick stamps, some new data have become available on troop movements and military history of the Pannonian ripa.
Cultural connections between the Eastern European steppe region and the Carpathian Basin in the 5th–7th centuries AD
Authors: Gulyás Bence
Published: March 2024

Extended, completed review of the theses of the PhD dissertation completed under the supervision of Tivadar Vida and submitted to the Archaeological Doctoral Programme, Doctoral School of History, ELTE Eötvös Loránd University, Budapest, in 2023.The dissertation’s primary objective was to investigate the origins of the population that settled in the Trans-Tisza region in the 6th–7th centuries AD. According to prior studies, these populations, unlike the ‘Inner Asian Avars’, may have arrived in the Carpathian Basin from the Eastern European steppe. My research focused on burial practices, comparing the graves discovered in the two regions using quantitative and qualitative methods. The dissertation also includes a comprehensive presentation and analysis of the East European steppe material from the second half of the 5th to the middle third of the 7th century AD.
Insight into the microstructure and mechanical properties of Cu-20wt%Zn-xSn ternary alloy
Authors: Ifeanacho U Okeke , Kingsley C Nnakwo , Eugene E Nnuka
Published: March 2024

The main objective of this research is to study the grain refinement and improved trend in mechanical properties of brass (Cu-20wt%Zn) doped with tin. Tin was added in concentrations of (x: 0.1, 0.3, 0.5, 0.8, and 1wt%). The alloy samples were produced by permanent die casting and machined to the required dimensions for the structural analysis and mechanical tests. Mechanical properties studied were percentage elongation, ultimate tensile strength, and Brinell hardness using a 100KN JPL tensile strength tester (Model: 130812) and portable dynamic hardness testing machine (Model: DHT-6) respectively. The structural analysis was conducted using an optical metallurgical microscope (Model: L2003A) and scanning electron microscopy (SEM) equipped with EDS. The results of the structural analysis showed that the control specimen consisted predominantly of coarse α-phase of zinc in copper (Cu3Zn). The samples doped with tin consisted of refined α-grains with patches of the second phase (Cu8Sn5). Mechanical test results indicated that the addition of tin to alpha brass improved the ultimate tensile strength and hardness with a slight decline in percentage elongation. The values recorded for ultimate tensile strength and Brinell hardness were 322MPa and 176HB respectively.
Long Amplicon Nanopore Sequencing for Dual-Typing RdRp and VP1 Genes of Norovirus Genogroups I and II in Wastewater v1
Authors: Scott George , Walker David
Published: March 2024

This protocol outlines the procedures for dual-typing (genotyping and polymerase typing) of norovirus genogroups I and II (GI and GII) from wastewater. It assumes that wastewater sample collection, viral concentration and nucleic acid extraction has already been performed. The wastewater samples used during method development were processed using an ammonium sulphate precipitation (150 mL sample) followed by nucleic acid extraction using Kingfisher Flex‱ (Thermo Scientific‱, UK) and NucliSENS‱ reagents (BioMérieux, France). Adapting this technique for use with differing viral concentration methods and nucleic acid extraction techniques should still be suitable. The analysis of different sample matrices will also likely be possible following sample processing (up to and including nucleic acid extraction) with methods appropriate for that matrix. This protocol starts with reverse transcription followed by semi-nested PCR amplifying the RdRp+VP1 regionof norovirus GI and GII independently. GI and GII amplicons are combined into a single library for simultaneous sequencing of the ≈1000 bp PCR products using either the Oxford Nanopore Technologies MinION or GridION. Consensus sequences are generated using NGSpeciedID, grouped at 95% then dual-typed giving both genotype and polymerase type (e.g.GI.2[P2]). Reads are aligned to consensus sequences and PCR chimeras are filtered using reference, de novo and manual approaches.
International Shipping Documentation v1
Authors: Islet Distribution Program Integrated
Published: March 2024

This SOP defines the completion of International Documentation necessary for the shipment of human islets and biospecimens to investigators outside of the United States for use in the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) sponsored research in the Integrated Islet Distribution Program (IIDP) using the United Parcel Service (UPS) as a courier. This is SOP is to be used in conjunction with other relevant IIDP SOPs previously published for shipping tissues using Federal Express for shipments within the United States: Packaging and Cold Shipping of Human Islets Collection, Packaging and Cold Shipping of Fresh Non-Islet Pancreatic (Acinar) Tissue Packaging and Shipping of Flash Frozen Non-Islet Pancreatic (Acinar)Tissue Collection, Broadcast, Packaging and Shipping of Non-Islet Biospecimens This protocol is written to assist the participating islet isolation centers who are part of this program.
Phenol-chloroform DNA extraction from Sporosarcina pasteurii v1
Authors: S. Carter Michael , J. Tuttle Matthew , K. Gupta Maneesh
Published: March 2024

This protocol is for extraction of genomic DNA from Sporosarcina pasteurii. It is based on a standard phenol-chloroform DNA extraction method.
Alt Ekstremite Yüzeyel Venöz Yetmezlik Tedavisinde 980 nm ve 1470 nm Lazer ile Endovenöz Lazer Ablasyon Tedavisinin Orta Dönem Sonuçları
Authors: Arı Murat , Büyük Ahmet , Bozoğlan Orhan , Eroğlu Erdinç
Published: March 2024

Amaç: Alt ekstremite yüzeyel venöz yetmezlik klinik semptomlara neden olan tedavi edilmediğinde venöz ülserlere kadar uzanabilen ve toplumda sık görülen bir hastalıktır. Yüzeyel venöz yetmezlik ve varislerin tedavisinde uzun yıllardır primer tedavi yöntemi cerrahi tedavidir. Endovenöz lazer ablasyon (EVLA) safen ven yetmezliği tedavisinde, son dönemde ön plana çıkan güvenli ve efektif minimal invaziv tedavi yöntemidir. Bu çalışmamızda, farklı dalga boylu lazerler ile EVLA tedavilerinin etkinliğini, komplikasyonlarını, erken ve orta dönem sonuçlarını karşılaştırmayı amaçladık.
Gereçler ve Yöntemler: Çalışmaya Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Kalp ve Damar Cerrahisi Kliniği’ne başvuran, semptomatik yüzeyel venöz yetersizlik tanısı alan ve tedavi endikasyonu konup EVLA planlanan 100 kişi dahil edildi. Hastalar 2 gruba ayrıldı. 25 hastanın bilateral hastalığı mevcuttu, toplam 125 alt ekstremiteye tedavi uygulandı. Bir gruba 980 nm dalga boylu 10W gücünde lazer (Grup 1), diğer gruba ise 1470 nm dalga boylu 10W gücünde lazer (Grup 2) uygulandı.
Bulgular: 6.ay kontrolünde 1470 nm dalga boylu lazer uygulanan grubun tamamında tam oklüzyon devam ederken, 980 nm dalga boylu lazer uygulanan hastaların 3 ekstremitesinde (%5) rekanalizasyon izlendi. Venöz klinik şiddet skoru (VCSS) ve VAS skorları işlem öncesi her 2 grupta da benzerdi. İşlem sonrası Grup 2'de hem VCSS (p=0,002) hem de VAS skoru (p<0,001) anlamlı düşük saptandı. Aynı şekilde günlük aktiviteye dönme süresi (p<0,001) ve ağrı duyma süresi (p<0,001) Grup 2’de daha kısaydı. Her 2 grupta da majör komplikasyon görülmezken, minör komplikasyon olarak kabul edilen ekimoz, parestezi, endürasyon ve analjezi ihtiyacı Grup 2’de daha az (sırasıyla p<0,0001, p<0,0001, p<0,0001, p<0,001) görülmüştür.
Sonuç: Bu çalışmamızda EVLA tedavisinde kullanılan yüksek lazer dalga boyunun düşük lazer dalga boyuna sahip lazerlere kıyasla daha etkin ve daha az postoperatif yan etkilere yol açtığı saptanmıştır.
AIMS TO DIGITALISE THE CIRCULATION OF PRECIOUS METALS, PRECIOUS STONES AND THEIR PRODUCTS
Authors: Umgaeva O. V. , Eldeev D. Kh. , Umgaev S. A.
Published: March 2024

The need to digitalise economic sectors in today’s world is perceived as an objective reality. In the circulation of precious metals, precious stones and their products, the digitalisation process is at a fairly high level, with continuous improvement. The article analyses the progress made in achieving the goals of the implementation of the State Integrated Information System in the Sphere of Control over the Circulation of Precious Metals and Precious Stones at all stages (GIIS DMDK), which is the main project of digitalisation of the industry.
The legal basis for ensuring national economic security on the example of assessing the socio-economic asymmetry of the subjects of the Russian Federation
Authors: Grudtsyna L. Yu.
Published: March 2024

In this article, an analysis of relevant, according to modern researchers, methods of assessing the socio-economic asymmetry of regions in the aspect of ensuring national economic security is carried out and their significance for the present development of the economy is revealed. As part of such an analysis, the interaction between the socio-economic development of the regions and national economic security is investigated, as well as the types of possible development of regions from which asymmetric is distinguished. Among the methodological foundations for assessing the socio- economic asymmetry of the regions of Russia, the article discusses and notes the dominant advantages of using the following methods: the method of expert assessments, the method of reformatting the territorial and administrative structure of the region to reduce asymmetry, assessing the impact of the industrial cluster on the socio-economic development of the region, SWOT analysis. As an example, calculations of socio-economic asymmetry were made based on statistical data for 2019 and 2020 in the Central and North Caucasus Federal Districts for the fundamental indicator of socio-economic development — the gross regional product. Using the results of the calculations, the relevance of the problem of interregional differentiation of socioeconomic development in Russia for the present period is assessed and some ways of solving such a problem are proposed, possible at this stage.
Are there objective biomechanical symptoms of the knee instability after anterior cruciate ligament injury or it’s reconstruction
Authors: Skvortsov Dmitry V. , Altukhova Alyona V. , Kaurkin Sergey N. , Akhpashev Alexander A.
Published: March 2024

BACKGROUND: The instability of the knee joint after a rupture of the anterior cruciate ligament and even after its reconstruction remains a pressing problem. The use of a special functional test with fast walking can be considered as a potential tool for verifying this instability.
AIM: to observe the presence of biomechanical differences in the joint function with and without instability, both before and after the anterior cruciate ligament reconstruction.
METHODS: A biomechanical study of the function of walking at fast speeds, including an electromyographic study, was used. 40 patients were examined, of which 22 underwent reconstruction of the anterior cruciate ligament. The patients were divided into two groups — 33 patients with symptoms of instability, and 7 patients without them; the control group included 20 healthy persons.
RESULTS: The time characteristics of the step cycle did not reveal significant differences between the patient groups and the control group. For the amplitudes of movements in the joints, there were also no significant differences between the patient groups and between the affected and intact sides. The only finding was a significant increase in both amplitudes in the knee joint compared to the control for the intact side in the group with instability. The electromyographic study also did not reveal significant differences.
CONCLUSION: The use of a functional test with fast walking does not allow differentiating the state of instability. Thus, the instability of the knee joint during normal walking, even at a high speed, does not manifest itself in any way. To detect it, it is necessary to develop special provocative tests.
Hypotensive therapy in obstetric practice
Authors: Ziganshin Aydar M. , Maksyutova Alina A.
Published: March 2024

Arterial hypertension remains a leading cause of maternal morbidity and mortality while leading to significant economic costs in health care. Properly selected treatment tactics allow for prolonging pregnancy and levelling out various complications arising from the disease. However, there are still some questions about the effectiveness and safety of prescribed therapy due to possible side effects of the drugs. The aim of this study was to evaluate the efficacy and complications of hypotensive therapy during pregnancy, childbirth and the postpartum period. We analyzed the literature on eLibrary, Scopus, Cochrane Library, PubMed.
Evaluation of renal function in patients with type 1 diabetes mellitus implementing reproductive function in assisted reproductive technology protocols
Authors: Veretekhina Tatiana V. , Yarmolinskaya Maria I.
Published: March 2024

The prevalence of type 1 diabetes mellitus has increased significantly among women of reproductive age over the past two decades. Despite improved glycemic control and intensified insulin therapy, patients with diabetes still suffer from many reproductive problems, which often makes this group of patients potential participants in assisted reproductive technology programs under certain conditions. Diabetic nephropathy is one of the most serious complications of type 1 diabetes mellitus. It ranks first in the structure of chronic kidney disease and is a common cause of end-stage renal failure, disability, and mortality. Early diagnosis and identification of specific markers of diabetic nephropathy will allow for timely initiation of nephroprotective therapy to slow the progression of diabetic kidney damage.
This review article is based on the results of the PubMed, Frontiers, and ResearchGate search queries from 2016 to 2023. We analyzed worldwide and domestic data on the impact of type 1 diabetes mellitus on kidney function, the influence of sex hormones on diabetic nephropathy, and the importance of the personalized approach to this group of patients at the pre-pregnancy stage, especially those planning treatment within assisted reproductive technology programs.
Life Path of Professor Elena Pavlovna Yakovleva (1938–2023)
Authors: Kolodyaznaya Vera A. , Topkova Oksana V. , Kotova Natalia  V.
Published: March 2024

This essay describes the life path of Professor Elena Pavlovna Yakovleva, a Doctor of Biological Sciences, who headed the Biotechnology Department at the Saint Petersburg Chemical Pharmaceutical Academy from 1993 to 2013. Elena Pavlovna is the author of over 30 inventions, more than 180 publications in leading Russian and international scientific journals, a co-author of the monograph “Regulation of the biosynthesis of biologically active substances,” and the textbook “Biotechnology.” E. P. Yakovleva’s works have been recognized with bronze awards from VDNKh of the USSR. In 2006, Elena Pavlovna Yakovleva was included in the list of top biotechnology experts published in the USA by Marquis Who’s Who magazine. Elena Pavlovna Yakovleva worked at the Saint Petersburg Chemical Pharmaceutical Academy until she was 80 years old. She was a talented scientist, valued and respected by her university colleagues, possessed a gentle character, high intellect, caring attitude towards staff and students, always showed interest in people’s problems, and endeavored to help anyone in need of her support. We would like to express our special gratitude to Maxim Evgenievich Yakovlev, Elena Pavlovna’s grandson, as well as her son and daughter-in-law, for their help in summarizing archival materials provided from the family archive.
Comparative evaluation of two methods of prolonged femoral nerve block using an elastomeric pump after knee arthroplasty: a prospective randomized controlled study
Authors: Krylov Sergey V. , Pasechnik Igor N.
Published: March 2024

BACKGROUND: The number of knee arthroplasty (KA) surgeries is increasing annually. These surgeries are associated with severe pain. The optimal method of choice in perioperative analgesia in these patients remains controversial. The use of regional analgesia, including prolonged analgesia, allows for high pain relief and increased patient satisfaction. Modern elastomeric pumps (EP) provide prolonged conduction analgesia, which significantly expands the possibilities of perioperative pain relief.
OBJECTIVE: To evaluate the effectiveness of prolonged femoral nerve block using EP with and without bolus after KA.
MATERIALS AND METHODS: This prospective randomized study included 75 patients who were divided into two clinical groups: group 1 (n=36), patients who received prolonged regional analgesia using EP with controlled injection without bolus, and group 2 (n=39), patients who received prolonged regional analgesia using EP with bolus. In the postoperative period, the level of pain at rest and during movement was assessed after 6, 12, 24, and 48 hours as well as the need for opioid analgesics, consumption of local anesthetic for various options of continued regional analgesia, quadriceps femoris muscle function, and distance traveled by patients after surgery in the first 2 days.
RESULTS: No significant differences were noted in pain level at rest and during movement in the observation period between the groups (p=0.213). No additional prescription of opioid analgesics was required. Local anesthetic consumption was lower in group 2 (group 1.384±33.4 ml; group 2.237±25.1 ml; p=0.031). Patients in group 2 had greater activity in knee extension and, thus, greater distance traveled after surgery.
CONCLUSION: The use of EP with a bolus has been demonstrated to provide adequate levels of analgesia and better functional recovery in patients with bolus after KA than in patients without bolus. This technique is recommended as a component of enhanced recovery program after surgery.