bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Mitochondrial Iron Overload in Mediating Cell Death in H9c2 Cells
Authors: Tam Eddie , Sung Hye Kyoung , Lam Nhat Hung , You Sally , Cho Sungji , Ahmed Saher M. , Abdul-Sater Ali A. , Sweeney Gary
Published: December 2022

Iron overload (IO) is associated with cardiovascular diseases, including heart failure. Our study’s aim was to examine the mechanism by which IO triggers cell death in H9c2 cells. IO caused accumulation of intracellular and mitochondrial iron as shown by the use of iron-binding fluorescent reporters, FerroOrange and MitoFerroFluor. Expression of cytosolic and mitochondrial isoforms of Ferritin was also induced by IO. IO-induced iron accumulation and cellular ROS was rapid and temporally linked. ROS accumulation was detected in the cytosol and mitochondrial compartments with CellROX, DCF-DA and MitoSOX fluorescent dyes and partly reversed by the general antioxidant N-acetyl cysteine or the mitochondrial antioxidant SkQ1. Antioxidants also reduced the downstream activation of apoptosis and lytic cell death quantified by Caspase 3 cleavage/activation, mitochondrial Cytochrome c release, Annexin V/Propidium iodide staining and LDH release of IO-treated cells. Finally, overexpression of MitoNEET, an outer mitochondrial membrane protein involved in the transfer of Fe-S clusters between mitochondrial and cytosol, was observed to lower iron and ROS accumulation in the mitochondria. These alterations were correlated with reduced IO-induced cell death by apoptosis in MitoNEET-overexpressing cells. In conclusion, IO mediates H9c2 cell death by causing mitochondrial iron accumulation and subsequent general and mitochondrial ROS upregulation.
Fecal Microbiota Transplantation Reduces Pathology and Improves Cognition in a Mouse Model of Alzheimer’s Disease
Authors: Elangovan Shalini , Borody Thomas J. , Holsinger R. M. Damian
Published: December 2022

Characterized by the presence of amyloid plaques, neurofibrillary tangles and neuroinflammation, Alzheimer’s disease (AD) is a progressive neurodegenerative disorder with no known treatment or cure. Global disease projections warrant an urgent and rapid therapeutic for the treatment of this devastating disease. Fecal microbiota transplantation (FMT) is a widely accepted and safely used treatment for recurrent Clostridium difficile infection and other metabolic diseases such as diabetes mellitus. FMT has also been demonstrated to be a possible AD therapeutic. We examined the potential of FMT for the treatment of AD in a robust, mouse model of the disease and report that a brief, 7-day treatment regimen demonstrated ‘plaque-busting’ and behavior-modifying effects in treated 5xFAD mice. Importantly, we show that donor age plays an important role in the efficacy of the treatment and these findings warrant further investigation in human trials.
Molecular Mechanisms and Clinical Application of Multipotent Stem Cells for Spinal Cord Injury
Authors: Szymoniuk Michał , Litak Jakub , Sakwa Leon , Dryla Aleksandra , Zezuliński Wojciech , Czyżewski Wojciech , Kamieniak Piotr , Blicharski Tomasz
Published: December 2022

Spinal Cord Injury (SCI) is a common neurological disorder with devastating psychical and psychosocial sequelae. The majority of patients after SCI suffer from permanent disability caused by motor dysfunction, impaired sensation, neuropathic pain, spasticity as well as urinary complications, and a small number of patients experience a complete recovery. Current standard treatment modalities of the SCI aim to prevent secondary injury and provide limited recovery of lost neurological functions. Stem Cell Therapy (SCT) represents an emerging treatment approach using the differentiation, paracrine, and self-renewal capabilities of stem cells to regenerate the injured spinal cord. To date, multipotent stem cells including mesenchymal stem cells (MSCs), neural stem cells (NSCs), and hematopoietic stem cells (HSCs) represent the most investigated types of stem cells for the treatment of SCI in preclinical and clinical studies. The microenvironment of SCI has a significant impact on the survival, proliferation, and differentiation of transplanted stem cells. Therefore, a deep understanding of the pathophysiology of SCI and molecular mechanisms through which stem cells act may help improve the treatment efficacy of SCT and find new therapeutic approaches such as stem-cell-derived exosomes, gene-modified stem cells, scaffolds, and nanomaterials. In this literature review, the pathogenesis of SCI and molecular mechanisms of action of multipotent stem cells including MSCs, NSCs, and HSCs are comprehensively described. Moreover, the clinical efficacy of multipotent stem cells in SCI treatment, an optimal protocol of stem cell administration, and recent therapeutic approaches based on or combined with SCT are also discussed.
Anti-Inflammatory Effect of Specialized Proresolving Lipid Mediators on Mesenchymal Stem Cells: An In Vitro Study
Authors: AlZahrani Shahd , Shinwari Zakia , Gaafar Ameera , Alaiya Ayodele , Al-Kahtani Ahmed
Published: December 2022

An interconnection between tissue inflammation and regeneration has been established through the regulation of defense and repair mechanisms within diseased dental tissue triggered by the release of immune-resolvent mediators. To better our understanding of the role of specific pro-resolving mediators (SPMs) in inflamed human bone marrow-derived mesenchymal stem cells (hBMMSCs), we studied the effects of Resolvin E1 (RvE1) and Maresin 1 (MaR1) in lipopoly-saccharide (LPS) stimulated hBMMSCs. The hBMMSCs were divided into five different groups, each of which was treated with or without SPMs. Group-1: negative control (no LPS stimulation), Group-2: positive control (LPS-stimulated), Group-3: RvE1 100 nM + 1 μg/mL LPS, Group-4: MaR1 100 nM + 1 µg/mL LPS, and Group-5: RvE1 100 nM + MaR1100 nM + 1 μg/mL LPS. Cell proliferation, apoptosis, migration, colony formation, Western blotting, cytokine array, and LC/MS analysis were all performed on each group to determine the impact of SPMs on inflammatory stem cells. According to our data, RvE1 plus MaR1 effectively reduced inflammation in hBMMSCs. In particular, IL-4, 1L-10, and TGF-β1 activation and downregulation of RANKL, TNF-α, and IFN-γ compared to groups receiving single SPM were shown to be significantly different (Group 3 and 4). In addition, the LC/MS analysis revealed the differentially regulated peptide’s role in immunological pathways that define the cellular state against inflammation. Inflamed hBMMSCs treated with a combination of Resolvin E1 (RvE1) and Maresin 1 (MaR1) promoted the highest inflammatory resolution compared to the other groups; this finding suggests a potential new approach of treating bacterially induced dental infections.
Potentiometric Determination of Free Fluoride Content in Wines from Dalmatia Region (Croatia)—A Comparative Study of Direct Potentiometry and Standard Addition Method
Authors: Buljac Maša , Bralić Marija , Vrca Ivana , Kolar Mitja , Radić Josip
Published: December 2022

The aim of this study was to investigate 30 different types of Dalmatian wines as a potential source of fluoride. A fluoride ion selective electrode was used to measure the fluoride concentration in each sample. The direct potentiometric method and the standard addition method were evaluated, the latter being suggested as more accurate and precise. Measurements were performed in two buffers, acetate buffer and total ionic strength adjustment buffer (TISAB), to compare their influence on fluoride determination. The obtained results show that TISAB is a better choice than acetate buffer as a medium for fluoride determination. According to the proposed method, mass concentrations of fluoride of 0.19 and 0.18 mg/L were found in the studied red and white wines, with standard deviations of 0.04 and 0.03 mg/L, respectively. All determined fluorine levels in the tested wines were within the recommended limits and do not pose a risk to human health. No significant difference was found between the fluorine content in white and red wines, but there was a difference depending on the place of origin of the wine. The measured pH values for all the wines studied (except one sample) are very similar and show no significant correlation with the fluoride content.
Advanced Mapping of Optically-Blind and Optically-Active Nitrogen Chemical Impurities in Natural Diamonds
Authors: Kudryashov Sergey , Rimskaya Elena , Kuzmin Evgeny , Kriulina Galina , Pryakhina Victoria , Muratov Andrey , Khmelnitskii Roman , Greshnyakov Evgeny , Danilov Pavel , Shur Vladimir
Published: December 2022

Natural diamonds with a rich variety of optically blind and optically active nitrogen impurity centers were explored at a nano/microscale on the surface and in bulk by a number of advanced chemical and structural analytical tools in order to achieve a comprehensive characterization by establishing enlightening links between their analysis results. First, novel compositional relationships were established between high-energy X-ray photoelectron spectroscopy (XPS) and low-energy Fourier-transform infrared vibrational spectroscopy (FT-IR) signals of nitrogen impurity defects acquired in the microscopy mode at the same positions of the diamond surface, indicating the verification XPS modality for qualitative and quantitative FT-IR analysis of high concentrations of nitrogen and other chemical impurity defects in diamond. Second, depth-dependent spatial distributions of diverse photoluminescence (PL)-active nitrogen defects were acquired in the confocal scanning mode in an octahedral diamond and then for the first time corrected to the related Raman signals of the carbon lattice to rule out artefacts of the confocal parameter and to reveal different micron-scale ontogenetic layers in the impurity distributions on its surface. Third, intriguing connections between local structural micro-scale defects (dislocation slip bands of plastic deformation zones) visualized by optical microscopy and Raman microspectroscopy, and related distributions of stress-sensitive PL-active nitrogen impurity defects in the proximity of these planes inside bulk diamonds were revealed. These findings demonstrate the broad instrumental opportunities for comprehensive in situ studies of the chemical, structural, and mechanical micro-features in diamonds, from the surface into bulk.
Compression-Only Cardiopulmonary Resuscitation and Automated External Defibrillator Course for Primary School Students: A Malaysian Pilot Study
Authors: Fariduddin Muhamad Nur , Mohamed Mawarni , Jaafar Mohd Johar , Baharin Kamarul , Siau Ching Sin , Bashah Kamal
Published: December 2022

The Malaysian national school curriculum currently lacks resources and tools to enforce CPR education. The aim of this study was to investigate the efficacy of a compression-only cardiopulmonary resuscitation and automated external defibrillator course among primary school students to increase their knowledge and technical skills and improve their attitudes. A quasi-experimental study was conducted using a pre–post non-equivalent design involving 38 students aged 10–12. Cardiopulmonary resuscitation (CPR) and automated external defibrillator (AED) knowledge, technical skills, and attitude towards CPR were assessed in a post test with three-month follow-up. Results of the MANOVA analysis showed significant differences in the level of knowledge (F = 10.29, p < 0.001) and attitude (F = 13.87, p < 0.001) based on the students’ age group at the time of the post test. The proportion of students who passed the technical skills component differed significantly by age (χ2 = 12.12; p = 0.002) and BMI (χ2 = 6.34; p = 0.041). No significant decay was reported in the total mean scores for knowledge, technical skills, and attitude (F = 0.727, p = 0.54) at 3-month follow-up. The course helped students perform CPR and utilize AED effectively while promoting a positive attitude with up to 3 months of retention, demonstrating the feasibility of extending the course within the Malaysian primary school curriculum.
Psychometric Properties of the Chinese Version of the Brief Interpersonal Competence Questionnaire for Adolescents
Authors: Huang Liuyue , Huang Junrun , Chen Zhichao , Jiang Weiwei , Zhu Yi , Chi Xinli
Published: December 2022

This study aimed to evaluate the psychometric properties of the Brief Interpersonal Competence Questionnaire (ICQ-15) administered to Chinese adolescents. A sample of 1705 adolescents (Mean age = 14.08, SD = 3.22, 46.5% male) completed a questionnaire including the Chinese version of the ICQ-15, as well as measurements of well-being, psychological resilience, and depression. To examine the psychometric properties of the ICQ-15, item analyses (item–total correlation and normality test), confirmatory factor analysis, concurrent validity analyses, multi-group analyses, and internal consistency analyses were performed. The results of the item analyses suggested a good item–total correlation, and the item scores were distributed approximately normally. The confirmatory factor analysis showed that the five-factor model had acceptable fit indices. The concurrent validity analyses indicated that the Chinese version of the ICQ-15 had a satisfactory concurrent validity. The multi-group analyses proved the measurement invariance across females and males, as well as participants in early, middle, and late adolescence. The ICQ-15 demonstrated satisfactory internal consistency reliability among Chinese adolescents. The ICQ-15 presents good psychometric properties and can be used to assess interpersonal competence in Chinese adolescents.
Conclusive Thoughts for a New Beginning
Authors: Chiappedi Matteo
Published: December 2022

When I was asked to name this Special Issue, I was both honored and worried, as being appointed the Guest Editor was a significant achievement and honor [...]
Assessment of Permanent First Molars in Children Aged 7 to 10 Years Old
Authors: Urvasizoglu Gelengul , Bas Aybike , Sarac Fatma , Celikel Peris , Sengul Fatih , Derelioglu Sera
Published: December 2022

Background: Dental caries is a chronic, infectious and preventable disease that is very common around the world. It has been observed that dental caries affect not only the majority of adults but also 60% to 90% of children. Permanent first molars (PFM) are the most commonly decayed teeth observed in children. Aim: The aim of this study is to evaluate the decayed, missing filled teeth (DMFT) scores of PFMs in the early post-eruptive stage, within the scope of the United Nations Agenda for 2030 Sustainable Development Goals, thereby raising awareness for the prevention and treatment of permanent tooth decay. Methods: This descriptive cross-sectional epidemiological study was conducted in Erzurum between the years 2015–2016 by collecting data from children aged 7–10 years (17,208). In addition to the decayed, filled and missing data of the students’ 6-year-molars, their ages, genders, frequencies of both tooth brushing and dental office visits were evaluated. The relationship between the variables was analyzed with chi-square. Result: The present study analyzed the data of a total of 11,457 children, 5704 girls and 5753 boys with a mean age of 8.74 ± 1.18. There was a statistically significant difference between the PFMs 16, 26, 36 and 46 regarding the number of healthy, decayed, missing and restored teeth (p < 0.001). Conclusion: In this study, the prevalence of caries in the PFMs of children aged 7–10 years was 15.9% and the mean DMFT was 0.79 ± 1.39. This result showed that PFMs might develop carious lesions and even be lost within three years in the early post-eruptive stage.
Childcare Subsidy Enrollment Income Generosity and Child Maltreatment
Authors: Klika J. Bart , Maguire-Jack Kathryn , Feely Megan , Schneider William , Pace Garrett T. , Rostad Whitney , Murphy Catherine A. , Merrick Melissa T.
Published: December 2022

In the United States, childcare subsidies are available to low-income working parents to assist with the cost of childcare. The subsidies are provided as block grants to states, which allows for a great deal of flexibility in the specific policies guiding their distribution. Prior research has found a protective link between childcare subsidies and child maltreatment, but the variations in policies have been much less explored. The current study used longitudinal administrative child welfare data from 10 years (2009–2019) linked with state policies regarding the income eligibility requirements of states to examine the impact of these policies on child abuse and neglect among young children (0–5); early school-age children (6–12), and older children (13–17). Using multiple regression and controlling for state demographic characteristics, the study found that more generous policies surrounding income eligibility were related to lower rates of child abuse and neglect investigations at the state level.
Maximizing Quality of Life in Children with Epilepsy
Authors: Ng Yu-Tze
Published: December 2022

Arguably significant progress and improvement in the medical and surgical treatments of seizures and epilepsy in children have occurred; however, there have been relatively fewer efforts in optimizing the care of lifestyle complications related to the disease state. Many patients have significant behavioral and mental health comorbidities, including ADHD (attention deficit hyperactivity disorder), which should be treated. After epilepsy surgery, only seizure freedom results in improved quality of life (QOL). Improved compliance leads to better seizure control and ensuring that caregivers have a rescue treatment helps empower patients. Education and improving seizure illness perception is beneficial. Cannabidiol may have benefits other than seizure control. The majority of children are mainly concerned about the stigma attached to having epilepsy. Driving affecting older children is discussed. Restrictions on these children should be minimized and enabling regular activities maximized.
Gestational Alloimune Liver Disease—Case Report
Authors: Demetrian Mihaela , Botezatu Radu , Gică Nicolae , Safta Valentina , Grecu Georgeta , Dima Vlad , Binișor Andreea Daniela , Panaitescu Anca
Published: December 2022

We describe the case of a newborn with the antenatal onset of hepatic failure, which has been investigated for all etiologies that can cause liver damage: infectious, metabolic, genetic, and immune. The lack of a clear answer regarding the etiology and the response to immunoglobulin therapy led us to the diagnosis of gestational alloimmune liver disease. Gestational alloimunne liver disease is an uncommon and very severe cause of neonatal acute liver failure (NALF). Initially, the therapeutic approach aimed at correcting the effects produced by iron loading, respectively, iron chelators and antioxidants. Since all aspects of this case indicated characteristic features typical for GALD, therapy with intravenous immunoglobulins (IVIG) was introduced. If such therapy alters the prognosis of newborns with GALD, the etiology and pathophysiology remain uncertain. However, in cases regarding severe hepatic failure with the perinatal onset and apparently unknown etiology, immunoglobulin or exchange transfusion therapy should be taken into account even before finalizing all the etiological investigations. The prognosis is uncertain and varies between clinical resolution, chronic hepatitis/cirrhosis, and the need for a hepatic transplant, and overall survival depends on prompt therapeutic intervention.
LSD1 for the Targeted Regulation of Adipose Tissue
Authors: Chen Li , Sun Xuan , Chen Daidi , Gui Qingjun
Published: December 2022

White and thermal (brown and beige) adipose tissue energy storage and oxidative regulation pathways play a central role in maintaining the energy balance throughout the body, and the dysregulation of these pathways is closely related to glucose and lipid metabolism disorders and adipose tissue dysfunction, including obesity, chronic inflammation, insulin resistance, mitochondrial dysfunction, and fibrosis. Recent epigenetic studies have identified the novel regulatory element LSD1, which controls the above parameters, and have provided new mechanistic possibilities for re-encoding the fate and function of adipocytes. In this review, we outline the current advances in adipocyte metabolism in physiology and disease and discuss possible strategies for LSD1 to alter the phenotype of adipose tissue and thus influence energy utilization to improve metabolic health.
Flow Cytometry Detection of Anthracycline-Treated Breast Cancer Cells: An Optimized Protocol
Authors: Catitti Giulia , De Fabritiis Simone , Brocco Davide , Simeone Pasquale , De Bellis Domenico , Vespa Simone , Veschi Serena , De Lellis Laura , Tinari Nicola , Verginelli Fabio , Marchisio Marco , Cama Alessandro , Patruno Antonia , Lanuti Paola
Published: December 2022

The use of anthracycline derivatives was approved for the treatment of a broad spectrum of human tumors (i.e., breast cancer). The need to test these drugs on cancer models has pushed the basic research to apply many types of in vitro assays, and, among them, the study of anthracycline-induced apoptosis was mainly based on the application of flow cytometry protocols. However, the chemical structure of anthracycline derivatives gives them a strong autofluorescence effect that must be considered when flow cytometry is used. Unfortunately, the guidelines on the analysis of anthracycline effects through flow cytometry are lacking. Therefore, in this study, we optimized the flow cytometry detection of doxorubicin and epirubicin-treated breast cancer cells. Their autofluorescence was assessed both by using conventional and imaging flow cytometry; we found that all the channels excited by the 488 nm laser were affected. Anthracycline-induced apoptosis was then measured via flow cytometry using the optimized setting. Consequently, we established a set of recommendations that enable the development of optimized flow cytometry settings when the in vitro assays of anthracycline effects are analyzed, with the final aim to reveal a new perspective on the use of those in vitro tests for the further implementation of precision medicine strategies in cancer.
The Role of Immune Checkpoint Inhibitors in Cancer Therapy
Authors: Basudan Ahmed M.
Published: December 2022

Over the years, immune checkpoint inhibitors (CPIs) have become a powerful treatment strategy in the field of cancer immunotherapy. In the last decade, the number of FDA-approved CPIs has been increasing prominently, opening new horizons for the treatment of a wide range of tumor types. Pointedly, three immune checkpoint molecules have been under extensive research, which include cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed cell death protein-1 (PD-1) and its ligand-1 (PD-L1). Despite remarkable success, not all patients respond positively to therapy, which highlights the complexity of the tumor microenvironment (TME) and immune system. This has led to the identification of molecular biomarkers to predict response and toxicity. In addition, there has been an emerging focus on developing new delivery and targeting approaches for better drug efficacy and potency. In this review, we highlight the mechanism of action of major CPIs, their clinical impact, variation in effectiveness, response prediction, updated clinical indications, current challenges and limitations, promising novel approaches, and future directions.
A Study on the Gap-Fill Process Deposited by the Deposition/Etch/Deposition Method in the Space-Divided PE-ALD System
Authors: Lee Baek-Ju , Seo Dong-Won , Choi Jae-Wook
Published: December 2022

This study concerns the development of a gap-fill process technology for isolating trench patterns. There are various gap-filling techniques in the case of trench patterns; nevertheless, a processing technology adopting the DED (deposition/etch/deposition) method was developed in this study. After the etch step, an Ar/O2 (1:2) plasma treatment technology reduced the residual amount of F in the films to 0.05%. By improving the etch uniformity, the deposition uniformity after the DED process on a 12-inch flat wafer was secured within <1%, and a high-quality SiO2 thin film with a dielectric constant of 3.97 and a breakdown field of 11.41 MV/cm was fabricated. The DED method can be used for gap-filling even in patterns with a high aspect ratio by changing process parameters, such as RF power and division of etch steps, according to the shape, depth, and CD size of the pattern. This study confirmed that a void-free gap-fill process can be developed in a trench pattern with a maximum aspect ratio of 40:1.
Effect of Welding Current on Wear Behavior of PTA-Welded Cu35Ni25Co25Cr15 HEA Coating
Authors: Gao Yang , Yang Zihan , Xiao Haibo , Lei Qian , Liu Bin , Liu Yong
Published: December 2022

High-entropy alloys (HEAs) have received increasing attention because of their excellent properties. To improve the surface hardness and wear resistance of pure Cu, a Cu35Ni25Co25Cr15 HEA coating was, for the first time, deposited on a pure Cu substrate by plasma transfer arc (PTA) welding. The welding current varied from 130 to 160 A. The microstructures and mechanical properties of the coating were investigated. The Cu35Ni25Co25Cr15 HEA coating comprised face-cubic-centered A1 (Cu-rich) and γ′ (NiCoCr-rich) phases. The coating had an apparent structural gradient with coarse grains at the interface with the Cu substrate. The bottom, middle, and top zones of the coating exhibited a fine smooth cellular structure, fine-needle structure, and long dendrites, respectively. As the welding current was increased, the dilution ratio of the coating and interfacial bonding strength gradually increased, whereas the hardness of the coating increased and then decreased. Under a welding current of 150 A, no gaps or pores appeared in the interface, and the coating exhibited good metallurgical bonding with the Cu substrate. The wear mechanisms of the Cu35Ni25Co25Cr15 HEA coating at different temperatures were also determined. Compared with pure Cu, the Cu35Ni25Co25Cr15 HEA coating exhibited excellent wear resistance, especially at high temperatures. This study paves a new way for coating on pure Cu and enhances the wear resistance of Cu components.
Facile Synthesis of Pyrrolyl-Containing Semisquaraines in Water as Precursors for Non-Symmetric Squaraines
Authors: Ta Daniel D. , Favret Jeanne M. , Dzyuba Sergei V.
Published: December 2022

One-step reactions between squaric acid and pyrroles, such as 3-ethyl-2,4-dimethyl-pyrrole and 1,2,5-trimethylpyrrole, in water provide the corresponding pyrrol-2-yl- and pyrrol-3-yl-containing semisquaraines in high yields. These semisquaraines serve as useful precursors for the synthesis of various non-symmetric pyrrole-containing squaraine dyes.
Repeat-Dose Toxicity of Candidate Omicron COVID-19 Vaccine in Sprague-Dawley Rats
Authors: Zhao Yuxiu , He Zhenyu , Wang Zhanhui , He Yao , Zhao Xue , Liang Hongyang , Guo Yancen , Wang Yichuan , Zhang Xiaonan , Zhu Huiqin , Dong Yuan , Liu Yingwei , Li Wanli , Ding Ling , Zhu Xiujuan , Lan Haoyue , Zhang Yuntao , Wang Hui , Yang Xiaoming
Published: December 2022

Due to a large number of mutations in the spike protein and immune escape, the Omicron variant (B.1.1.529) has become a predominant variant of concern (VOC) strain. To prevent the disease, we developed a candidate inactivated vaccine (Omicron COVID-19 Vaccine (Vero Cell), Inactivated). To evaluate the safety of the vaccine, we tested the repeat-dose toxicity in Sprague-Dawley (SD) rats. The doses were administered randomly to three groups: physiological saline solution (control), aluminum adjuvant in PBS solution adjuvant (adjuvant group), and low-dose and high-dose omicron vaccines (vaccine group) for 6 weeks. The SD rats were allowed to recover for 4 weeks after withdrawal. We evaluated the physiological condition of the rats, including their ophthalmological condition, body weight, food intake, body temperature, blood biochemistry, urine, neutralizing antibody, inflammation at the injection site, and organs weight. In summary, no dose-dependent adverse toxicological changes were observed, and a recovery trend was obvious, which proved the preclinical safety of the candidate omicron vaccine and provided evidence for clinical trials in humans.
A 0D/2D Heterojunction Composite of Polymeric Carbon Nitride and ZIF-8-Derived ZnO for Photocatalytic Organic Pollutant Degradation
Authors: Viswanathan Vandana , Balakrishnan Vaishak , Adarsh Nayarassery , Varghese V Binsy , Mathew Suresh
Published: December 2022

Solar photocatalytic technology based on semiconducting materials has gained the attention of the scientific community to solve the energy crisis and environmental remediation. Zeolitic imidazolate frameworks (ZIFs) are a subfamily of metal–organic frameworks (MOFs) with the isomorphic topologies of zeolites and coordinative compositions of MOFs. Owing to high specific surface areas, tunable channels and high thermal stabilities, zeolitic imidazolate frameworks (ZIFs) have been used in catalytic applications. In this paper, ZIF-8 was used as a matrix to synthesize 0D/2D heterojunction photocatalysts, viz., ZnO/C3N4-x% (x = 2.5, 5 and 10), for the photocatalytic degradation study of rhodamine B (RhB). The synthesized composite materials were characterized using FTIR, PXRD, UVDRS, PL, TEM, and BET analyses. TEM images showed the nearby contacts between ZnO and C3N4 in the hybrid and the uniform distribution of ZnO on the surface of the C3N4 nanosheet, thus increasing the development of 0D/2D heterojunction. The hybrid system ZnO/C3N4-5% (ZCN-5) showed good photocatalytic activity for the degradation of RhB under sunlight. A possible mechanism for the improved photocatalytic activity of the ZnO/C3N4 composite is also suggested. This exploratory study demonstrates the effective separation and migration of photo-induced electron–hole pairs between the 2D C3N4 sheet and 0D ZnO for the improved performance of heterojunction photocatalysts.
Evaluating the Effect of 18O Incorporation on the Vibrational Spectra of Vaterite and Calcite
Authors: King Helen E. , Živković Aleksandar , de Leeuw Nora H.
Published: December 2022

Calcium carbonates are critical in biomineralization processes and as functional materials. For many applications, isotope enrichment in these materials allows researchers to monitor reaction pathways and retrace environmental signatures. When using vibrational spectroscopy, isotopic composition is currently derived by summing the concentration of each isotopologue, assumed to be directly obtainable from the band intensity, divided by the content of the isotope within the different isotopologues (e.g., C16O3, C16O218O, C16O18O2 and C18O3). However, this approach relies on the assumption that each isotopologue band has an equivalent intensity when present at the same concentration within the crystal structure. Here, using a joint experimental and theoretical approach we test the spectral behavior of the O-isotopologues by examining the effect of a key isotopic tracer, 18O, on the vibrational spectra of the calcium carbonate phases calcite and vaterite. We demonstrate that isotopic substitution changes both band positions and band intensities to different extents, depending on the vibrational spectroscopy method used and the bands examined. For calcite, the υ1 symmetrical stretching Raman-active bands related to individual isotopologues are found to have very similar intensities and are not affected by changes in isotopologue distribution within the material. Fitting these bands resulted in a consistent underestimation of the isotopic enrichment of only 1%, thus they are expected to be useful for estimating 18O-enrichment extent in future experimental work. In contrast, vaterite vibrational bands change more extensively and thus cannot be used directly to determine the 18O concentration within the material. These results are expected to contribute to a deeper und less ambiguous understanding of evaluating isotopic enrichment effects in the vibrational spectra of calcium carbonates.
Structural Evolution from Neutron Powder Diffraction of Nanostructured SnTe Obtained by Arc Melting
Authors: Gainza Javier , Serrano-Sánchez Federico , Rodrigues João E. F. S. , Dura Oscar J. , Fragoso Brenda , Ferrer Mateus M. , Nemes Norbert M. , Martínez José L. , Fernández-Díaz María T. , Alonso José A.
Published: December 2022

Among chalcogenide thermoelectric materials, SnTe is an excellent candidate for intermediate temperature applications, in replacement of toxic PbTe. We have prepared pure polycrystalline SnTe by arc melting, and investigated the structural evolution by temperature-dependent neutron powder diffraction (NPD) from room temperature up to 973 K. In this temperature range, the sample is cubic (space group Fm-3m) and shows considerably larger displacement parameters for Te than for Sn. The structural analysis allowed the determination of the Debye model parameters and provided information on the Sn–Te chemical bonds. SEM images show a conspicuous nanostructuration in layers below 30 nm thick, which contributes to the reduction of the thermal conductivity down to 2.5 W/m·K at 800 K. The SPS treatment seems to reduce the number of Sn vacancies, thus diminishing the carrier density and increasing the Seebeck coefficient, which reaches 60 μV K−1 at 700 K, as well as the weighted mobility, almost doubled compared with that of the as-grown sample.
Mineralogical Characteristics Study of Calcite from the Fujian Province, China
Authors: Zhao Zhe-Yi , Lin Yu-Tao , Zhao Yi , Xu Bo
Published: December 2022

With mineral in situ testing technology and ore deposit geochemistry development, calcite has become a hot topic in studying carbonate minerals. Four large-grain calcite crystals from Fujian, China, were used for a detailed study. This study provides a comprehensive data set through mineralogical standard properties and spectral characteristics, including Fourier transform infrared, X-ray fluorescence spectrum, and Raman spectroscopy. Major elements were analyzed using X-ray fluorescence (XRF) and Micro-XRF. A high-resolution gas-source stable isotope ratio mass spectrometer was used to test C-O isotope characteristics. The four samples’ spectral characteristics and phase composition show excellent uniformity, all with the same characteristic peaks, indicating that they are pure calcite without other impurity minerals. C-O isotope characteristics indicate that Fujian calcite has mantle genetic characteristics, which may be affected by marine carbonate dissolution or sedimentary rock contamination. The variation of Fe and Mn contents may indicate that the four samples formed at different metallogenic stages.
Crystal Structure, Hirshfeld Surface Analysis, In-Silico and Antimycotic Investigations of Methyl 6-methyl-4-(4-nitrophenyl)-2-oxo-1,2-dihydropyrimidine-5-carboxylate
Authors: Huseynzada Alakbar , Mori Matteo , Meneghetti Fiorella , Israyilova Aygun , Guney Elif , Sayin Koray , Chiarelli Laurent R. , Demiralp Mustafa , Hasanova Ulviyya , Abbasov Vagif
Published: December 2022

Herein, we report the preparation of methyl 6-methyl-4-(4-nitrophenyl)-2-oxo-1,2-dihydropyrimidine-5-carboxylate 2, obtained by the regioselective oxidative dehydrogenation of the dihydropyrimidine derivative 1 in the presence of cerium ammonium nitrate. The structure of compound 2 was investigated by single-crystal X-ray diffraction (SC-XRD), which allowed the determination of its tautomeric form. Moreover, the presence of non-covalent interactions and their impact on the crystal structure were analyzed. To better characterize the intermolecular contacts, the Hirshfeld surface and enrichment ratio analyses were performed. Furthermore, the antimycotic activity of compounds 1 and 2 was investigated against Candida albicans, Aspergillus flavus, and Aspergillus niger, and their efficacy was compared to that of fluconazole. Computational investigations on the putative target of the compounds provided insights to explain the better activity of 2 with respect to its synthetic precursor.
PERSIST: A Multimodal Dataset for the Prediction of Perceived Exertion during Resistance Training
Authors: Albert Justin Amadeus , Herdick Arne , Brahms Clemens Markus , Granacher Urs , Arnrich Bert
Published: December 2022

Measuring and adjusting the training load is essential in resistance training, as training overload can increase the risk of injuries. At the same time, too little load does not deliver the desired training effects. Usually, external load is quantified using objective measurements, such as lifted weight distributed across sets and repetitions per exercise. Internal training load is usually assessed using questionnaires or ratings of perceived exertion (RPE). A standard RPE scale is the Borg scale, which ranges from 6 (no exertion) to 20 (the highest exertion ever experienced). Researchers have investigated predicting RPE for different sports using sensor modalities and machine learning methods, such as Support Vector Regression or Random Forests. This paper presents PERSIST, a novel dataset for predicting PERceived exertion during reSIStance Training. We recorded multiple sensor modalities simultaneously, including inertial measurement units (IMU), electrocardiography (ECG), and motion capture (MoCap). The MoCap data has been synchronized to the IMU and ECG data. We also provide heart rate variability (HRV) parameters obtained from the ECG signal. Our dataset contains data from twelve young and healthy male participants with at least one year of resistance training experience. Subjects performed twelve sets of squats on a Flywheel platform with twelve repetitions per set. After each set, subjects reported their current RPE. We chose the squat exercise as it involves the largest muscle group. This paper demonstrates how to access the dataset. We further present an exploratory data analysis and show how researchers can use IMU and ECG data to predict perceived exertion.
Parkinson’s Disease-Related Brain Metabolic Pattern Is Expressed in Schizophrenia Patients during Neuroleptic Drug-Induced Parkinsonism
Authors: Kotomin Ivan , Korotkov Alexander , Solnyshkina Irina , Didur Mikhail , Cherednichenko Denis , Kireev Maxim
Published: December 2022

Drug-induced parkinsonism (DIP) is a frequent parkinsonian syndrome that appears as a result of pharmacotherapy for the management of psychosis. It could substantially hamper treatment and therefore its diagnosis has a direct influence on treatment effectiveness. Although of such high importance, there is a lack of systematic research for developing neuroimaging-based criteria for DIP diagnostics for such patients. Therefore, the current study was aimed at applying a metabolic brain imaging approach using the 18F-FDG positron emission tomography and spatial covariance analysis to reveal possible candidates for DIP markers. As a result, we demonstrated, to our knowledge, the first attempt at the application of the Parkinson’s Disease-Related Pattern (PDRP) as a metabolic signature of parkinsonism for the assessment of PDRP expression for schizophrenia patients with DIP. As a result, we observed significant differences in PDRP expression between the control group and the groups with PD and DIP patients. Similar differences in PDRP expression were also found when the non-DIP schizophrenia patients were compared with the PD group. Therefore, our findings made it possible to conclude that PDRP is a promising tool for the development of clinically relevant criteria for the estimation of the risk of developing DIP.
Spectrum of Bacterial Pathogens from Urinary Infections Associated with Struvite and Metabolic Stones
Authors: Halinski Adam , Bhatti Kamran Hassan , Boeri Luca , Cloutier Jonathan , Davidoff Kaloyan , Elqady Ayman , Fryad Goran , Gadelmoula Mohamed , Hui Hongyi , Petkova Kremena , Popov Elenko , Rawa Bapir , Saltirov Iliya , Spivacow Francisco Rodolfo , Hameed Belthangady Monu Zeeshan , Arkusz Katarzyna , Trinchieri Alberto , Buchholz Noor
Published: December 2022

Objective: The purposes of this multi-center study were to evaluate the rate of infection stones and to evaluate the urine cultures of patients with infection stones. Materials: Charts of adulpatients with urinary stones were reviewed and data on stone analyses and urine cultures were collected. Results: In total, 1204 renal stone formers (RSFs) from 10 countries were included (776 males, 428 females). Fifty-six patients (4.6%) had struvite stones. The highest frequency of struvite stones was observed in India (23%) and Pakistan (18%). Lower rates were reported in Canada (2%), China (3%), Argentina (3%), Iraq (3%), Italy (3.5%) and Poland (3%), and intermediate rates in Egypt (5.5%) and Bulgaria (5.4%). Urine cultures were retrieved from 508 patients. Patients with struvite stones had a positive culture in 64.3% of the samples and patients with other stones, in 26.7%. In struvite stones, the most common isolates were Escherichia coli (27.7%) and Proteus spp. (27.7%), followed by Klebsiella spp. (16.7%); in other types of stone, it was Escherichia coli (47.6%), followed by Gram-positive bacteria (14.0%) Conclusions: The struvite stone composition was associated with a urinary infection, although an infection was not demonstrable with a conventional midstream urine culture in about 30%.
Role of Diphosphonates Bone Scintigraphy in Correlation with Biomarkers for a Personalized Approach to ATTR Cardiac Amyloidosis in North-Eastern Romania
Authors: Ionescu Teodor Marian , Ciocoiu Manuela , Lupușoru Raoul-Vasile , Grierosu Irena , Sascău Radu Andy , Jalloul Wael , Iacob Roxana , Stolniceanu Cati Raluca , Clement Alexandra , Stătescu Ana-Maria , Crișu Daniela , Petriș Antoniu Octavian , Mitu Florin , Ștefănescu Cipriana
Published: December 2022

Transthyretin cardiac amyloidosis (ATTR) is a rare cardiac protein deposition disease characterized by progressive thickening of both ventricles, the inter-atrial-ventricular septum and the atrioventricular valves. The gold standard method for diagnosing this rare pathology is endomyocardial biopsy. If this method cannot be used, the alternative is a mixture of clinical and paraclinical tests. Over the course of five years, we examined 58 patients suspected of cardiac amyloidosis based on electrocardiography and ultrasonography criteria, who had been sent for bone scintigraphy in order to determine the presence of ATTR cardiac amyloidosis. However, the final diagnosis was set by correlating the bone scan with genetic testing, free light chain dosage or soft tissue biopsy. Based on the final diagnosis we analyzed the patients’ predominant biomarkers in order to determine a possible correlation between them. This analysis is designed to help the general practitioner set a possible cardiac amyloidosis diagnosis.
Evaluation of the Analytical Performances of the Biolabo SOLEA 100 Optical Coagulometer and Comparison with the Stago STA-R MAX Analyser in the Determination of PT, APTT, and Fibrinogen
Authors: Bellio Pierangelo , De Angelis Simonetta , Piccirilli Alessandra , Di Michele Giulio , Barnabei Remo , Amicosante Gianfranco , Perilli Mariagrazia , Celenza Giuseppe
Published: December 2022

Introduction. The Biolabo Solea 100 is a fully automated coagulation analyser using an optical system to detect coagulation designed to meet the needs of small- and medium-sized laboratories. This study aimed to evaluate the analytical performance in terms of bias, precision, and interference of the Biolabo Solea 100 coagulometer under routine laboratory conditions. In addition, a comparison was made with Stago STA-R MAX. Materials and Methods. Imprecision and bias were evaluated for activated partial thromboplastin time (APTT), fibrinogen (FIB), and prothrombin time (PT) at the medical decision levels. The results of 200, 181, and 206 plasma samples for APTT, FIB, and PT, respectively, were compared with those obtained by Stago STA-R MAX. In addition, the interference level of bilirubin, haemoglobin, triglycerides, and fractionated heparin was evaluated. Results. Repeatability, intermediate imprecision, bias, and total error are overall below the defined limits of acceptability. Of interest is the high degree of agreement between Solea 100 and STA-R MAX with respect to PT (s), which fits perfectly with the theoretical line of identity (y = 0 + 1.00x). No interferences were found within the limits stated by the manufacturer, with some exceptions for APTT with heparin and APTT and PT for higher bilirubin concentrations. Conclusions. In conclusion, the performance of the Solea 100 optical analyser is satisfactory and adequate for the determination of routine coagulation tests. Moreover, they are perfectly comparable to mechanical systems, such as STA-R MAX and other upper-level analysers, even considering the low interference levels under routine conditions.
Autonomous Tracking of ShenZhou Reentry Capsules Based on Heterogeneous UAV Swarms
Authors: Li Boxin , Liu Boyang , Han Dapeng , Wang Zhaokui
Published: December 2022

The safe landing and rapid recovery of the reentry capsules are very important to manned spacecraft missions. A variety of uncertain factors, such as flight control accuracy and wind speed, lead to a low orbit prediction accuracy and a large landing range of reentry capsules. It is necessary to realize the autonomous tracking and continuous video observation of the reentry capsule during the low-altitude phase. Aiming at the Shenzhou return capsule landing mission, the paper proposes a new approach for the autonomous tracking of Shenzhou reentry capsules based on video detection and heterogeneous UAV swarms. A multi-scale video target detection algorithm based on deep learning is developed to recognize the reentry capsules and obtain positioning data. A self-organizing control method based on virtual potential field is proposed to realize the cooperative flight of UAV swarms. A hardware-in-the-loop simulation system is established to verify the method. The results show that the reentry capsule can be detected in four different states, and the detection accuracy rate of the capsule with parachute is 99.5%. The UAV swarm effectively achieved autonomous tracking for the Shenzhou reentry capsule based on the position obtained by video detection. This is of great significance in the real-time searching of reentry capsules and the guaranteeing of astronauts’ safety.
Solving HNP with One Bit Leakage: An Asymmetric Lattice Sieving Algorithm
Authors: Shi Wenhao , Jiang Haodong , Ma Zhi
Published: December 2022

The Hidden Number Problem (HNP) was introduced by Boneh and Venkastesan to analyze the bit-security of the Diffie–Hellman key exchange scheme. It is often used to mount a side-channel attack on (EC)DSA. The hardness of HNP is mainly determined by the number of nonce leakage bits and the size of the modulus. With the development of lattice reduction algorithms and lattice sieving, the range of practically vulnerable parameters are extended further. However, 1-bit leakage is still believed to be challenging for lattice attacks. In this paper, we proposed an asymmetric lattice sieving algorithm that can solve HNP with 1-bit leakage. The algorithm is composed of a BKZ pre-processing and a sieving step. The novel part of our lattice sieving algorithm is that the lattice used in these two steps have different dimensions. In particular, in the BKZ step we use more samples to derive a better lattice basis, while we just use truncated lattice basis for the lattice sieving step. To verify our algorithm, we use it to solve HNP with 1-bit leakage and 116-bit modulus.