bluuebunny/crossref_metadata_2025 huggingface dataset:
A 56–161 GHz Common-Emitter Amplifier with 16.5 dB Gain Based on InP DHBT Process
Authors: Hou Yanfei , Yu Weihua , Yu Qin , Wang Bowu , Sun Yan , Cheng Wei , Zhou Ming
Published: July 2021

This paper presents a broadband amplifier MMIC based on 0.5 µm InP double-heterojunction bipolar transistor (DHBT) technology. The proposed common-emitter amplifier contains five stages, and bias circuits are used in the matching network to obtain stable high gain in a broadband range. The measurement results demonstrate a peak gain of 19.5 dB at 146 GHz and a 3 dB bandwidth of 56–161 GHz (relative bandwidth of 96.8%). The saturation output power achieves 5.9 and 6.5 dBm at 94 and 140 GHz, respectively. The 1 dB compression output power is −4.7 dBm with an input power of −23 dBm at 94 GHz. The proposed amplifier has a compact chip size of 1.2 × 0.7 mm2, including DC and RF pads.
Funding and Delivery Models for Modern Energy Cooking Services in Displacement Settings: A Review
Authors: Bisaga Iwona , To Long Seng
Published: July 2021

Cooking with modern energy fuels and technologies has a high potential to positively impact the users’ health and well-being, and make cooking safer and less burdensome for women and girls. To date, there have been numerous interventions targeting improved cooking solutions in displacement settings, but very few which have involved modern energy cooking, such as ethanol, biogas, LPG or electric cooking. They have been largely absent from humanitarian programming due to limited availability, affordability and lack of business models that suit those complex settings. Additionally, energy access services in displacement settings have historically relied primarily on grant-based funding. However, grants are limited to relatively short timeframes which do not align with the long-term needs of the displaced. New ways of funding energy access in displacement settings, and particularly modern energy cooking services, are urgently needed to address the scale of the challenge as the number of displaced surpassed 80 million in 2020, with close to 90% having little or no access to adequate cooking fuels and technologies. In this paper, we review modern energy cooking in displacement settings and the common ways of funding and delivering them. We argue that new ways of funding and delivering energy access in displacement settings are urgently needed to address the scale of the challenge and to facilitate transitions to modern energy cooking fuels and technologies, in line with Sustainable Development Goal 7 and the principle of ‘leaving no one behind’.
The Effect of Using Social Media in the Modern Marketing Communication on the Shaping an External Employer’s Image
Authors: Baruk Agnieszka Izabela , Wesołowski Grzegorz
Published: July 2021

The aim of this article was to determine the significance of modern marketing communication channels used in the process of shaping the external image of an enterprise as an employer. An analysis of the world literature on marketing, management, marketing communication and human resource management was used to prepare the theoretical part. The results of the analysis indicate a cognitive and research gap regarding the use of modern communication channels for building the external image of an enterprise in the role of an employer. In order to reduce the gap, empirical studies were conducted among young Polish potential employees, in which the survey method was used to gather primary data. The collected data were subjected to statistical analysis, during which the following methods and statistical tests were applied: the analysis of average values, exploratory factor analysis, Kruskal–Wallis test (KW), Pearson chi-square independence test and V-Cramer coefficient analysis. The results of the analyses conducted indicate, inter alia, that statistically significant diversity was identified in the case of non-professional media in terms of respondents’ opinions on whether the employer’s image created by modern media is better than the employer’s image created on the basis of classical marketing communication channels. In the case of professional and non-professional media, the age of the respondents was not a differentiating feature. Moreover, neither for professional media nor for non-professional media were statistically significant dependencies identified between respondents’ opinions on the impact of actions undertaken by enterprises on shaping their positive external image as an employer and respondents’ opinions on whether the employer’s image created on the basis of modern marketing communication channels is more beneficial than the employer’s image created on the basis of classical marketing communication channels. The results obtained on the basis of the research have a cognitive and applicability value, characterized by originality. Until now, the importance of using modern marketing communication channels in shaping the employer’s external image has not been analysed. This also applies to enterprises operating on the energy market.
Frequency Stability Issues and Research Opportunities in Converter Dominated Power System
Authors: Shrestha Ashish , Gonzalez-Longatt Francisco
Published: July 2021

Stable power supply has become a crucial thing in the current era of technology and automation. Although the power system has multiple stability issues and causes, frequency fluctuation plays a vital role in normal operation, whereby a system with significant frequency deviation can lead to the needless blackouts of the whole power system. With the rapid growth in power electronic converter (PEC)-based technologies and the huge penetration of nonsynchronous generators, the modern power system is becoming more complex by the day. This paper provides a comprehensive study on the stability issues that occur in modern power systems, mainly due to PEC-based technology integration. The in-depth reasons and the impacts of unstable power systems, along with their controlling techniques, are discussed to generate a clear understanding. Furthermore, the importance of frequency stability in a power system is discussed with respect to some important events that occurred in the past. This paper also discusses some potential techniques that could be performed to overcome the existing and/or upcoming challenges in the upgrading power system.
Study of the Intelligent Control and Modes of the Arctic-Adopted Wind–Diesel Hybrid System
Authors: Elistratov Viktor , Konishchev Mikhail , Denisov Roman , Bogun Inna , Grönman Aki , Turunen-Saaresti Teemu , Lugo Afonso Julian
Published: July 2021

For energy supply in the Arctic regions, hybrid systems should be designed and equipped to ensure a high level of renewable energy penetration. Energy systems located in remote Arctic areas may experience many peculiar challenges, for example, due to the limited transport options throughout the year and the lack of qualified on-site maintenance specialists. Reliable operation of such systems in harsh climatic conditions requires not only a standard control system but also an advanced system based on predictions concerning weather, wind, and ice accretion on the blades. To satisfy these requirements, the current work presents an advanced intelligent automatic control system. In the developed control system, the transformation, control, and distribution of energy are based on dynamic power redistribution, dynamic control of dump loads, and a bi-directional current transducer. The article shows the architecture of the advanced control system, presents the results of field studies under the standard control approach, and models the performance of the system under different operating modes. Additionally, the effect of using turbine control to reduce the effects of icing is examined. It is shown that the advanced control approach can reduce fuel consumption in field tests by 22%. Moreover, the proposed turbine control scheme has the potential to reduce icing effects by 2% to 5%.
Personalized Anti-Vibration Protection for Telematics Devices in Urban Freight Transport Vehicles
Authors: Wojnowski Jacek , Chmiel Jarosław
Published: July 2021

Vibrations are a major cause of human health disorders, circuit boards and machinery damage. Vibration dampers are considered to be the best option to counter these issues. Three-dimensional printing techniques play an increasingly important role in manufacturing small polymer parts with tailored properties. Thermoplastic elastomers (TPE) constitute a perfect material for manufacturing small-scale series absorber prototypes due to their thermoplastic nature, good elasticity and damping properties. This paper proposes a novel multi-level approach to the design and manufacturing process, e.g., the first level—selection of material; second level—decision about the geometry of a damper; third—selection of technological printing parameters; fourth—post-printing treatment. This work primarily aims to overview the design and manufacturing process levels. The impact of each step on the damping capacity of small absorbers is assessed. It was found that thermoplastic elastomers and fused deposition modeling (FDM) have huge potential in shaping the physical properties of small, elastomeric absorbers. It was assessed that at every step of the multilevel design and manufacturing process (MDMP), the designer could tailor the damping to meet the desired criteria of a final product: a cylinder-shaped hollow damper that can be made from TPE polymer without post-printing treatment and is characterized by good damping.
Three-Dimensional Rogue Waves in Earth’s Ionosphere
Authors: El-Taibany Wael F. , El-Bedwehy Nabila A. , El-Shafeay Nora A. , El-Labany Salah K.
Published: July 2021

The modulational instability of ion-acoustic waves (IAWs) in a four-component magneto-plasma system consisting of positive–negative ions fluids and non-Maxwellian (r,q) distributed electrons and positrons, is investigated. The basic system of fluid equations is reduced to a three-dimensional (3D) nonlinear Schrödinger Equation (NLS). The domains of the IAWs stability are determined and are found to be strongly affected by electrons and positrons spectral parameters r and q and temperature ratio Tp/Te (Tp and Te are positrons and electrons temperatures, respectively). The existence domains, where we can observe the ion-acoustic rogue waves (IARWs) are determined. The basic features of IARWs are analyzed numerically against the distribution parameters and the other system physical parameters as Tp/Te and the external magnetic field strength. Moreover, a comparison between the first- and second-order rogue waves solution is presented. Our results show that the nonlinearity of the system increases by increasing the values of the non-Maxwellian parameters and the physical parameters of the system. This means that the system gains more energy by increasing r, q, Tp, and the external magnetic field through the cyclotron frequency ωci. Finally, our theoretical model displays the effect of the non-Maxwellian particles on the MI of the IAWs and RWs and its importance in D–F regions of Earth’s ionosphere through (H+,O2−) and (H+,H−) electronegative plasmas.
Favored Inflationary Models by Scalar Field Condensate Baryogenesis
Authors: Kirilova Daniela , Panayotova Mariana
Published: July 2021

We calculate the baryon asymmetry value generated in the Scalar Field Condensate (SCF) baryogenesis model obtained in several inflationary scenarios and different reheating models. We provide analysis of the baryon asymmetry value obtained for more than 70 sets of parameters of the SCF model and the following inflationary scenarios, namely: new inflation, chaotic inflation, Starobinsky inflation, MSSM inflation, quintessential inflation. We considered both cases of efficient thermalization after inflation and delayed thermalization. We have found that the SFC baryogenesis model produces baryon asymmetry orders of magnitude bigger than the observed one for the following inflationary models: new inflation, new inflation model by Shafi and Vilenkin, MSSM inflation, chaotic inflation with high reheating temperature and the simplest Shafi–Vilenkin chaotic inflationary model. Strong diluting mechanisms are needed for these models to reduce the resultant baryon excess at low energies to its observational value today. We have found that a successful generation of the observed baryon asymmetry is possible by the SCF baryogenesis model in Modified Starobinsky inflation, chaotic inflation with low reheating temperature, chaotic inflation in SUGRA, and Quintessential inflation.
Assessing the Urban Eco-Environmental Quality by the Remote-Sensing Ecological Index: Application to Tianjin, North China
Authors: Zhang Ting , Yang Ruiqing , Yang Yibo , Li Long , Chen Longqian
Published: July 2021

The remote-sensing ecological index (RSEI), which is built with greenness, moisture, dryness, and heat, has become increasingly recognized for its use in urban eco-environment quality assessment. To improve the reliability of such assessment, we propose a new RSEI-based urban eco-environment quality assessment method where the impact of RSEI indicators on the eco-environment quality and the seasonal change of RSEI are examined and considered. The northern Chinese municipal city of Tianjin was selected as a case study to test the proposed method. Landsat images acquired in spring, summer, autumn, and winter were obtained and processed for three different years (1992, 2005, and 2018) for a multitemporal analysis. Results from the case study show that both the contributions of RSEI indicators to eco-environment quality and RSEI values vary with the season and that such seasonal variability should be considered by normalizing indicator measures differently and using more representative remote-sensing images, respectively. The assessed eco-environment quality of Tianjin was, overall, improving owing to governmental environmental protection measures, but the damage caused by rapid urban expansion and sea reclamation in the Binhai New Area still needs to be noted. It is concluded that our proposed urban eco-environment quality assessment method is viable and can provide a reliable assessment result that helps gain a more accurate understanding of the evolution of the urban eco-environment quality over seasons and years.
Global Contraction and Local Strengthening of Firms’ Supply and Sales Logistics Networks in the Context of COVID-19: Evidence from the Development Zones in Weifang, China
Authors: Yan Yiran , Wang Xingping
Published: July 2021

The stagnation of multinational and cross-regional goods circulation has created significant disruptions to manufacturing supply chains due to the outbreak of the COVID-19 pandemic. To explore the impact of COVID-19 on the circulation of manufacturing industry products at different geographical scales, we drew upon a case study of development zones in the city of Weifang in China to analyze the characteristics of firms’ logistics networks in these development zones, and how these characteristics have changed since the outbreak of the COVID-19 pandemic. The data used in this study were collected from fieldwork conducted between 26 August 2020 and 15 October 2020, and included the supply originations of firms’ manufacturing sources and the sales destinations of their goods. We chose the two-mode network analysis method as our study methodology, which separates the logistics networks into supply networks and sales networks. The results show the following: First, the overall structure of firms’ logistics networks in Weifang’s development zones is characterized by localization. In the context of the COVID-19 pandemic, the local network links have further strengthened, whereas the global links have seriously declined. Moreover, the average path length of both the supply and sales logistics networks has slightly decreased, indicating the increased connectivity of the logistics networks. Second, in terms of the network node centrality, the core nodes of the supply logistics networks are the development zones and the city in which the firms are located, whereas the core nodes of the sales logistics networks are the core companies in the development zones. However, since the outbreak of the COVID-19 pandemic, the centrality of supply originations and sales destinations at the local scale has increased, whereas the centrality of supply originations and sales destinations at the global scale has decreased significantly. Third, the influencing factors of such changes include controlling personnel and goods circulation based on national boundaries and administrative boundaries, forcing the logistics networks in the development zones to shrink to the local scale. Moreover, there are differences in the scope of spatial contraction between supply logistics networks and the sales logistics networks.
Megalin, Proton Pump Inhibitors and the Renin–Angiotensin System in Healthy and Pre-Eclamptic Placentas
Authors: Sun Yuan , Tan Lunbo , Neuman Rugina I. , Broekhuizen Michelle , Schoenmakers Sam , Lu Xifeng , Danser A. H. Jan
Published: July 2021

Soluble Fms-like tyrosine kinase-1 (sFlt-1) is increased in pre-eclampsia. The proton pump inhibitor (PPI) lowers sFlt-1, while angiotensin increases it. To investigate whether PPIs lower sFlt-1 by suppressing placental renin–angiotensin system (RAS) activity, we studied gene expression and protein abundance of RAS components, including megalin, a novel endocytic receptor for prorenin and renin, in placental tissue obtained from healthy pregnant women and women with early-onset pre-eclampsia. Renin, ACE, ACE2, and the angiotensin receptors were expressed at identical levels in healthy and pre-eclamptic placentas, while both the (pro)renin receptor and megalin were increased in the latter. Placental prorenin levels were upregulated in pre-eclamptic pregnancies. Angiotensinogen protein, but not mRNA, was detectable in placental tissue, implying that it originates from maternal blood. Ex vivo placental perfusion revealed a complete washout of angiotensinogen, while prorenin release remained constant. The PPI esomeprazole dose-dependently reduced megalin/(pro)renin receptor-mediated renin uptake in Brown Norway yolk sac epithelial cells and decreased sFlt-1 secretion from placental villous explants. Megalin inhibition blocked angiotensinogen uptake in epithelial cells. In conclusion, our data suggest that placental RAS activity depends on angiotensinogen taken up from the maternal systemic circulation. PPIs might interfere with placental (pro)renin-AGT uptake/transport, thereby reducing angiotensin formation as well as angiotensin-induced sFlt-1 synthesis.
Applications of Radiolabelled Curcumin and Its Derivatives in Medicinal Chemistry
Authors: Mari Matteo , Carrozza Debora , Ferrari Erika , Asti Mattia
Published: July 2021

Curcumin is a natural occurring molecule that has aroused much interest among researchers over the years due to its pleiotropic set of biological properties. In the nuclear medicine field, radiolabelled curcumin and curcumin derivatives have been studied as potential radiotracers for the early diagnosis of Alzheimer’s disease and cancer. In the present review, the synthetic pathways, labelling methods and the preclinical investigations involving these radioactive compounds are treated. The studies entailed chemical modifications for enhancing curcumin stability, as well as its functionalisation for the labelling with several radiohalogens or metal radionuclides (fluorine-18, technetium-99m, gallium-68, etc.). Although some drawbacks have yet to be addressed, and none of the radiolabelled curcuminoids have so far achieved clinical application, the studies performed hitherto provide useful insights and lay the foundation for further developments.
The Role of Extracellular Carbonic Anhydrase in Biogeochemical Cycling: Recent Advances and Climate Change Responses
Authors: Mustaffa Nur Ili Hamizah , Latif Mohd Talib , Wurl Oliver
Published: July 2021

Climate change has been predicted to influence the marine phytoplankton community and its carbon acquisition strategy. Extracellular carbonic anhydrase (eCA) is a zinc metalloenzyme that catalyses the relatively slow interconversion between HCO3− and CO2. Early results indicated that sub-nanomolar levels of eCA at the sea surface were sufficient to enhance the oceanic uptake rate of CO2 on a global scale by 15%, an addition of 0.37 Pg C year−1. Despite its central role in the marine carbon cycle, only in recent years have new analytical techniques allowed the first quantifications of eCA and its activity in the oceans. This opens up new research areas in the field of marine biogeochemistry and climate change. Light and suitable pH conditions, as well as growth stage, are crucial factors in eCA expression. Previous studies showed that phytoplankton eCA activity and concentrations are affected by environmental stressors such as ocean acidification and UV radiation as well as changing light conditions. For this reason, eCA is suggested as a biochemical indicator in biomonitoring programmes and could be used for future response prediction studies in changing oceans. This review aims to identify the current knowledge and gaps where new research efforts should be focused to better determine the potential feedback of phytoplankton via eCA in the marine carbon cycle in changing oceans.
Compatibility of Distinct Label-Free Proteomic Workflows in Absolute Quantification of Proteins Linked to the Oocyte Quality in Human Follicular Fluid
Authors: Lewandowska Aleksandra E. , Fel Anna , Thiel Marcel , Czaplewska Paulina , Łukaszuk Krzysztof , Wiśniewski Jacek R. , Ołdziej Stanisław
Published: July 2021

We present two separate label-free quantitative workflows based on different high-resolution mass spectrometers and LC setups, which are termed after the utilized instrument: Quad-Orbitrap (nano-LC) and Triple Quad-TOF (micro-LC) and their directed adaptation toward the analysis of human follicular fluid proteome. We identified about 1000 proteins in each distinct workflow using various sample preparation methods. With assistance of the Total Protein Approach, we were able to obtain absolute protein concentrations for each workflow. In a pilot study of twenty samples linked to diverse oocyte quality status from four donors, 455 and 215 proteins were quantified by the Quad-Orbitrap and Triple Quad-TOF workflows, respectively. The concentration values obtained from both workflows correlated to a significant degree. We found reasonable agreement of both workflows in protein fold changes between tested groups, resulting in unified lists of 20 and 22 proteins linked to oocyte maturity and blastocyst development, respectively. The Quad-Orbitrap workflow was best suited for an in-depth analysis without the need of extensive fractionation, especially of low abundant proteome, whereas the Triple Quad-TOF workflow allowed a more robust approach with a greater potential to increase in effectiveness with the growing number of analyzed samples after the initial effort of building a comprehensive spectral library.
Molecular Mechanisms of Antiproliferative and Apoptosis Activity by 1,5-Bis(4-Hydroxy-3-Methoxyphenyl)1,4-Pentadiene-3-one (MS13) on Human Non-Small Cell Lung Cancer Cells
Authors: Wan Mohd Tajuddin Wan Nur Baitty , Abas Faridah , Othman Iekhsan , Naidu Rakesh
Published: July 2021

Diarylpentanoid (DAP), an analog that was structurally modified from a naturally occurring curcumin, has shown to enhance anticancer efficacy compared to its parent compound in various cancers. This study aims to determine the cytotoxicity, antiproliferative, and apoptotic activity of diarylpentanoid MS13 on two subtypes of non-small cell lung cancer (NSCLC) cells: squamous cell carcinoma (NCI-H520) and adenocarcinoma (NCI-H23). Gene expression analysis was performed using Nanostring PanCancer Pathways Panel to determine significant signaling pathways and targeted genes in these treated cells. Cytotoxicity screening revealed that MS13 exhibited greater inhibitory effect in NCI-H520 and NCI-H23 cells compared to curcumin. MS13 induced anti-proliferative activity in both cells in a dose- and time-dependent manner. Morphological analysis revealed that a significant number of MS13-treated cells exhibited apoptosis. A significant increase in caspase-3 activity and decrease in Bcl-2 protein concentration was noted in both MS13-treated cells in a time- and dose-dependent manner. A total of 77 and 47 differential expressed genes (DEGs) were regulated in MS13 treated-NCI-H520 and NCI-H23 cells, respectively. Among the DEGs, 22 were mutually expressed in both NCI-H520 and NCI-H23 cells in response to MS13 treatment. The top DEGs modulated by MS13 in NCI-H520—DUSP4, CDKN1A, GADD45G, NGFR, and EPHA2—and NCI-H23 cells—HGF, MET, COL5A2, MCM7, and GNG4—were highly associated with PI3K, cell cycle-apoptosis, and MAPK signaling pathways. In conclusion, MS13 may induce antiproliferation and apoptosis activity in squamous cell carcinoma and adenocarcinoma of NSCLC cells by modulating DEGs associated with PI3K-AKT, cell cycle-apoptosis, and MAPK pathways. Therefore, our present findings could provide an insight into the anticancer activity of MS13 and merits further investigation as a potential anticancer agent for NSCLC cancer therapy.
Enzalutamide Enhances PSMA Expression of PSMA-Low Prostate Cancer
Authors: Staniszewska Magdalena , Fragoso Costa Pedro , Eiber Matthias , Klose Jasmin M. , Wosniack Jasmin , Reis Henning , Szarvas Tibor , Hadaschik Boris , Lückerath Katharina , Herrmann Ken , Fendler Wolfgang P. , Iking Janette
Published: July 2021

Prostate-specific membrane antigen (PSMA)-directed radioligand therapy (RLT) prolongs overall survival in men with metastatic castration-resistant prostate cancer (mCRPC). However, men with low PSMA expression are excluded from RLT. We explored the effect of androgen receptor blockade with enzalutamide on PSMA expression. Assessment of PSMA and androgen receptor (AR) expression on the human PC cell lines 22Rv1, C4-2, and LNCaP by immunohistochemistry and flow cytometry revealed low (22Rv1) and high (C4-2 and LNCaP) PSMA expression, and high, comparable AR positivity. Treatment with enzalutamide increased PSMA levels in 22Rv1, C4-2, and LNCaP (2.2/2.3/2.6-fold, p = 0.0005/0.03/0.046) after one week compared to DMSO-treated controls as assessed by flow cytometry. NOD/Scid mice bearing 22Rv1 tumors were treated with enzalutamide for two weeks. Positron emission tomography/computed tomography (PET/CT) demonstrated higher tumor uptake of 68Ga-PSMA after enzalutamide treatment (p = 0.004). Similarly, a clinical case with low baseline PSMA avidity demonstrated increased uptake of 68Ga-PSMA after enzalutamide on PET/CT and post-therapeutic 177Lu-PSMA scintigraphy in a patient with mCRPC. Enzalutamide induced PSMA expression in the 22Rv1 xenograft model and in an mCRPC patient, both with low baseline tumoral PSMA levels. Therefore, enzalutamide pre-treatment might render patients with low PSMA expression eligible for 177Lu-PSMA RLT.
Physiological and Biochemical Response to Fusarium culmorum Infection in Three Durum Wheat Genotypes at Seedling and Full Anthesis Stage
Authors: Pastuszak Jakub , Szczerba Anna , Dziurka Michał , Hornyák Marta , Kopeć Przemysław , Szklarczyk Marek , Płażek Agnieszka
Published: July 2021

Fusarium culmorum is a worldwide, soil-borne plant pathogen. It causes diseases of cereals, reduces their yield, and fills the grain with toxins. The main direction of modern breeding is to select wheat genotypes the most resistant to Fusarium diseases. This study uses seedlings and plants at the anthesis stage to analyze total soluble carbohydrates, total and cell-wall bound phenolics, chlorophyll content, antioxidant activity, hydrogen peroxide content, mycotoxin accumulation, visual symptoms of the disease, and Fusarium head blight index (FHBi). These results determine the resistance of three durum wheat accessions. We identify physiological or biochemical markers of durum wheat resistance to F. culmorum. Our results confirm correlations between FHBi and mycotoxin accumulation in the grain, which results in grain yield decrease. The degree of spike infection (FHBi) may indicate accumulation mainly of deoxynivalenol and nivalenol in the grain. High catalase activity in the infected leaves could be considered a biochemical marker of durum sensitivity to this fungus. These findings allowed us to formulate a strategy for rapid evaluation of the disease severity and the selection of plants with higher level, or resistance to F. culmorum infection.
Nrf2, the Major Regulator of the Cellular Oxidative Stress Response, is Partially Disordered
Authors: Karunatilleke Nadun C. , Fast Courtney S. , Ngo Vy , Brickenden Anne , Duennwald Martin L. , Konermann Lars , Choy Wing-Yiu
Published: July 2021

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription regulator that plays a pivotal role in coordinating the cellular response to oxidative stress. Through interactions with other proteins, such as Kelch-like ECH-associated protein 1 (Keap1), CREB-binding protein (CBP), and retinoid X receptor alpha (RXRα), Nrf2 mediates the transcription of cytoprotective genes critical for removing toxicants and preventing DNA damage, thereby playing a significant role in chemoprevention. Dysregulation of Nrf2 is linked to tumorigenesis and chemoresistance, making Nrf2 a promising target for anticancer therapeutics. However, despite the physiological importance of Nrf2, the molecular details of this protein and its interactions with most of its targets remain unknown, hindering the rational design of Nrf2-targeted therapeutics. With this in mind, we used a combined bioinformatics and experimental approach to characterize the structure of full-length Nrf2 and its interaction with Keap1. Our results show that Nrf2 is partially disordered, with transiently structured elements in its Neh2, Neh7, and Neh1 domains. Moreover, interaction with the Kelch domain of Keap1 leads to protection of the binding motifs in the Neh2 domain of Nrf2, while the rest of the protein remains highly dynamic. This work represents the first detailed structural characterization of full-length Nrf2 and provides valuable insights into the molecular basis of Nrf2 activity modulation in oxidative stress response.
The Effects of Royal Jelly Acid, 10-Hydroxy-trans-2-decenoic Acid, on Neuroinflammation and Oxidative Stress in Astrocytes Stimulated with Lipopolysaccharide and Hydrogen Peroxide
Authors: Ali Amira Mohammed , Kunugi Hiroshi
Published: July 2021

The increased prevalence of neurodegenerative diseases, especially during the COVID-19 outbreak, necessitates the search for natural immune- and cognitive-enhancing agents. 10-Hydroxy-trans-2-decenoic acid (10-H2DA), the main fatty acid of royal jelly, has several pharmacological activities. Given the fundamental role of astrocytes in regulating immune responses of the central nervous system, we used cortical astrocytes to examine the effect of 10-H2DA on the expression of genes associated with neuroinflammation and the production of neurotrophins, as well as cellular resistance to H2O2-induced cytotoxicity. Astrocytes, pretreated with a range of concentrations of 10-H2DA for 24 h, were exposed to lipopolysaccharide (LPS) for 3 h, after which the expression of proinflammatory cytokines (IL-1β, IL-6, and tumor necrosis factor-α (TNF-α)) and neurotrophic factors (BDNF, GDNF, and IGF-1) was evaluated. In the absence of LPS, 10-H2DA had no significant effect on the mRNA expression of neurotrophins or cytokines except for IL-1β, which significantly increased with low doses of 10-H2DA (3 µM). 10-H2DA (10 µM) pretreatment of LPS-stimulated cells did not significantly inhibit the expression of cytokine encoding genes; however, it significantly lowered the mRNA expression of GDNF and tended to decrease BDNF and IGF-1 expression compared with LPS alone. Additionally, 10-H2DA did not protect astrocytes against H2O2-induced oxidative stress. Our data indicate no anti-inflammatory, antioxidant, or neurotrophic effect of 10-H2DA in astrocytes undergoing inflammation or oxidative stress. The effect of IGF-1 inhibition by 10-H2DA on neuronal ketogenesis needs investigation.
The Assessment of IL-21 and IL-22 at the mRNA Level in Tumor Tissue and Protein Concentration in Serum and Peritoneal Fluid in Patients with Ovarian Cancer
Authors: Mielczarek-Palacz Aleksandra , Kruszniewska-Rajs Celina , Smycz-Kubańska Marta , Strzelczyk Jarosław , Szanecki Wojciech , Witek Andrzej , Gola Joanna Magdalena
Published: July 2021

The aim of the analysis was for the first time to assess the expression of genes encoding IL-21 and IL-22 at the mRNA level in ovarian tumor specimens and the concentration of these parameters in serum and peritoneal fluid in patients with ovarian serous cancer. The levels of IL-21 and IL-22 transcripts were evaluated with the use of the real-time RT-qPCR. Enzyme-linked immunosorbent assay (ELISA) was used to determine the concentration of proteins. Quantitative analysis of IL-21 gene mRNA in the tumor tissue showed the highest activity in the G1 degree of histopathological differentiation and was higher in G1 compared to the control group. The concentration of IL-21 and IL-22 in the serum and in the peritoneal fluid of women with ovarian cancer varied depending on the degree of histopathological differentiation of the cancer and showed statistical variability compared to controls. The conducted studies have shown that the local and systemic changes in the immune system involving IL-21 and IL-22 indicate the participation of these parameters in the pathogenesis of ovarian cancer, and modulation in the IL-21/IL-22 system may prove useful in the development of new diagnostic and therapeutic strategies used in patients, which require further research.
Outcomes of Kidney Transplant Recipients with Sickle Cell Disease: An Analysis of the 2000–2019 UNOS/OPTN Database
Authors: Leeaphorn Napat , Thongprayoon Charat , Vaitla Pradeep , Hansrivijit Panupong , Jadlowiec Caroline C. , Mao Shennen A. , Chewcharat Api , Katari Sreelatha , Pattharanitima Pattharawin , Boonpheng Boonphiphop , Kaewput Wisit , Mao Michael A. , Cooper Matthew , Cheungpasitporn Wisit
Published: July 2021

Background: Lower patient survival has been observed in sickle cell disease (SCD) patients who go on to receive a kidney transplant. This study aimed to assess the post-transplant outcomes of SCD kidney transplant recipients in the contemporary era. Methods: We used the OPTN/UNOS database to identify first-time kidney transplant recipients from 2010 through 2019. We compared patient and allograft survival between recipients with SCD (n = 105) vs. all other diagnoses (non-SCD, n = 146,325) as the reported cause of end-stage kidney disease. We examined whether post-transplant outcomes improved among SCD in the recent era (2010–2019), compared to the early era (2000–2009). Results: After adjusting for differences in baseline characteristics, SCD was significantly associated with lower patient survival (HR 2.87; 95% CI 1.75–4.68) and death-censored graft survival (HR 1.98; 95% CI 1.30–3.01), compared to non-SCD recipients. The lower patient survival and death-censored graft survival in SCD recipients were consistently observed in comparison to outcomes of recipients with diabetes, glomerular disease, and hypertension as the cause of end-stage kidney disease. There was no significant difference in death censored graft survival (HR 0.99; 95% CI 0.51–1.73, p = 0.98) and patient survival (HR 0.93; 95% CI 0.50–1.74, p = 0.82) of SCD recipients in the recent versus early era. Conclusions: Patient and allograft survival in SCD kidney recipients were worse than recipients with other diagnoses. Overall SCD patient and allograft outcomes in the recent era did not improve from the early era. The findings of our study should not discourage kidney transplantation for ESKD patients with SCD due to a known survival benefit of transplantation compared with remaining on dialysis. Urgent future studies are needed to identify strategies to improve patient and allograft survival in SCD kidney recipients. In addition, it may be reasonable to assign risk adjustment for SCD patients.
Changes in Chinese Adults’ Physical Activity Behavior and Determinants before and during the COVID-19 Pandemic
Authors: Wang Huan , Feng Lianshi , Zhang Yanfeng , Zhang Fuhong , Fu Jinmei , Wang Mei , Wu Dongming , Feng Qiang , Liu Xinhua , Fan Chaoqun , Wang Jingjing , Gao Weizhen , McDonough Daniel J. , Gao Zan
Published: July 2021

Purpose: To investigate the changes in Chinese adults’ physical activity (PA) behavior and determinants before and during the COVID-19 pandemic. Method: A total of 1028 adults (aged 19–59 years) were recruited from 127 urban and rural neighborhoods in China using stratified three-stage probability sampling. Data collection was conducted in December 2019 and July 2020. Results: Compared with the data before the pandemic, individuals’ weekly moderate-to-vigorous-intensity PA (MVPA) decreased significantly from 139 min to 120 min, seven months after the outbreak (p = 0.01), with female and rural populations displaying a more significant decrease (p = 0.02). Overall, 13.7% of participants met the PA guidelines (World Health Organization) both before and during the pandemic, while 21.8% met the guidelines only before the pandemic and 18.1% increased their PA and met the PA guidelines during the pandemic. A total of 46.4% did not meet the PA guidelines before or during the pandemic. Determinants of PA behavior change before and during the pandemic included sports skills, self-determined motivation and support from sports organizations. Conclusions: The Chinese adults’ PA levels decreased significantly from before to during the COVID-19 pandemic, particularly among the female population. It is suggested that the enhancement of self-determined motivation, improvement of sport skills, and support from sports organizations might be effective in facilitating individuals’ engagement in PA during the pandemic.
Main Drivers of Fecundity Variability of Mussels along a Latitudinal Gradient: Lessons to Apply for Future Climate Change Scenarios
Authors: Oliveira Gabriela F. , Siregar Hanifah , Queiroga Henrique , Peteiro Laura G.
Published: July 2021

Bivalve relevance for ecosystem functioning and human food security emphasize the importance of predictions of mussel performance under different climate stressors. Here, we address the effect of a latitudinal gradient of temperature and food availability on the fecundity of the Mediterranean mussel to try to better parameterize environmental forcing over reproductive output. We show that temperature plays a major role, acting as a switching on–off mechanism for gametogenesis, while food availability has a lower influence but also modulates the number of gametes produced. Temperature and food availability also show different effects over fecundity depending on the temporal scale evaluated. Our results support the view that the gametogenesis responds non-linearly with temperature and chlorophyll concentration, an issue that is largely overlooked in growth, production and energy budgets of bivalve populations, leading to predictive models that can overestimate the capability of the mussel’s populations to deal with climate change future scenarios.
Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement
Authors: Madzak Catherine
Published: July 2021

Among non-conventional yeasts of industrial interest, the dimorphic oleaginous yeast Yarrowia lipolytica appears as one of the most attractive for a large range of white biotechnology applications, from heterologous proteins secretion to cell factories process development. The past, present and potential applications of wild-type, traditionally improved or genetically modified Yarrowia lipolytica strains will be resumed, together with the wide array of molecular tools now available to genetically engineer and metabolically remodel this yeast. The present review will also provide a detailed description of Yarrowia lipolytica strains and highlight the natural biodiversity of this yeast, a subject little touched upon in most previous reviews. This work intends to fill this gap by retracing the genealogy of the main Yarrowia lipolytica strains of industrial interest, by illustrating the search for new genetic backgrounds and by providing data about the main publicly available strains in yeast collections worldwide. At last, it will focus on exemplifying how advances in engineering tools can leverage a better biotechnological exploitation of the natural biodiversity of Yarrowia lipolytica and of other yeasts from the Yarrowia clade.
Potential Activity of Albino Grifola frondosa Mushroom Extract against Biofilm of Meticillin-Resistant Staphylococcus aureus
Authors: Fasciana Teresa , Gargano Maria Letizia , Serra Nicola , Galia Elena , Arrigo Ignazio , Tricoli Maria Rita , Diquattro Orazia , Graceffa Giuseppa , Vieni Salvatore , Venturella Giuseppe , Giammanco Anna
Published: July 2021

Mushroom extracts are a rich source of natural compounds with antimicrobial properties, which are able to prevent, to some extent, the growth of foodborne pathogens. The aim of this study was to investigate the potential of extracts from albino Grifola frondosa (GF), commonly known as maitake, to inhibit the growth of some bacteria and the biofilm production by Staphylococcus aureus. We obtained not only a significant reduction of OD score between biofilm and biofilm plus albino G. frondosa extract group, but also a reduction of category of biofilm. In addition, we observed a significant presence of isolates with strong category for the biofilm group and a significant presence of isolates with absent category for the biofilm plus albino G. frondosa extract group. These results confirm that the use of albino G. frondosa extract reduces in significant way the presence of biofilm. Our results suggest and confirm that albino G. frondosa extracts could be employed as functional food and could be used as a natural additive for food process control and food safety.
The Digitalization of Motion Picture Production and Its Value Chain Implications
Authors: Schulz Anne , Eder Amelie , Tiberius Victor , Solorio Samantha Casas , Fabro Manuela , Brehmer Nataliia
Published: July 2021

Technological change and development have been ongoing in the motion picture industry since its beginnings some 125 years ago. What further advancements of digitalization can be expected over the next decade and what are its implications for the industry’s value chain? To answer this question, we conducted an international two-stage Delphi study. The results suggested a more frequent use of smartphones as cameras, the emergence of full digital film sets and digital star avatars, as well as advancements in VR-based and interactive movies. The findings imply challenges for traditional players in the motion picture value chain. Production technology becomes both simpler and more complex, leading to the threat of new entrants.
Architectural and Urban Attractiveness of Small Towns: A Case Study of Polish Coastal Cittaslow Towns on the Pomeranian Way of St. James
Authors: Zawadzka Alicja K.
Published: July 2021

The paper presents the results of a study on the attractiveness to tourists and natives of the cultural qualities of coastal towns on The Pomeranian Way of St. James that are members of the Cittaslow network. Attention to the quality of urban life is inscribed in the development policies of towns applying to join the Cittaslow movement. In order to join the network (apart from the size criterion), towns need to meet a minimum of 50% plus one of the 72 criteria grouped into seven categories. One of the category is Quality of Urban Life Policy, so the towns applying to join Cittaslow commit themselves to actions aimed at improving the quality of urban life. The study on the attractiveness of cultural qualities of towns to tourists and natives was conducted using the author’s BRB method, whose added value is its universality and the possibility to study small towns regardless of their membership in the Cittaslow network. BRB is an acronym that stands for BUILDINGS, RELATIONSHIPS, BALANCE, and comprises three scopes of activities: BUILDINGS (iconic building and important sites where the inhabitants and the tourists are present); RELATIONSHIPS (the visual effects of the relations between the inhabitants and the town) and BALANCE (solutions that implement modern technologies). This method enables identification of places that are important to the inhabitants, where urban life takes place and which are often created with the involvement of the inhabitants. These are often the same spaces as those that attract tourists and perhaps stimulate them the desire to visit the town again (BRB—be right back). The aim of the BRB method is shown the attractiveness of small towns. The study has shown that the characteristic feature of Polish Cittaslow towns is their diversity: the architectural attractiveness of three towns is high both to tourists and natives. On the other hand, the urban attractiveness of the examined towns is an insufficient.
Soil Quality Assessment after 25 Years of Sewage Sludge vs. Mineral Fertilization in a Calcareous Soil
Authors: Simoes-Mota Ana , Poch Rosa Maria , Enrique Alberto , Orcaray Luis , Virto Iñigo
Published: July 2021

The aim of this work was to identify the most sensitive soil quality indicators and assess soil quality after long-term application of sewage sludge (SS) and conventional mineral fertilization for rainfed cereal production in a sub-humid Mediterranean calcareous soil. The treatments included six combinations of SS at different doses (40 t ha−1 and 80 ha−1) and frequencies (every 1, 2 and 4 years), plus a control with mineral fertilization, and a baseline control without fertilization. Twenty-five years after the onset of the experiment, 37 pre-selected physical, chemical and biological soil parameters were measured, and a minimum data set was determined. Among these indicators, those significantly affected by treatment and depth were selected as sensitive. A principal component analysis (PCA) was then performed for each studied depth. At 0–15 cm, PCA identified three factors (F1, F2 and F3), and at 15–30 cm, two factors (F4 and F5) that explained 71.5% and 67.4% of the variation, respectively, in the soil parameters. The most sensitive indicators (those with the highest correlation within each factor) were related to nutrients (P and N), organic matter, and trace metals (F1 and F4), microporosity (F2), earthworm activity (F3), and exchangeable cations (F5). Only F3 correlated significantly (and negatively) with yield. From these results, we concluded that soil quality can be affected in opposite directions by SS application, and that a holistic approach is needed to better assess soil functioning under SS fertilization in this type of agrosystem.
Can Agricultural Disaster Relief Programs Affect Farmland Prices? Empirical Evidence from Farmland Transaction Data
Authors: Lee Tzong-Haw , Chang Hung-Hao , Hsieh Yi-Ting , Chang Bo-Yuan
Published: July 2021

The literature on the capitalization of agricultural policies documents that government subsidies can increase farmland values with attesting empirical evidence found in a variety of agricultural programs. This study argues that the well-documented capitalization effect of agricultural subsidies on farmland prices may not be directly related to the agricultural disaster relief program (ADRP). On the one hand, disaster relief payments can positively capitalize into farmland prices. On the other hand, disaster shocks may result in farm income loss which can decrease farmland prices. This paper empirically examines the overall effect of the ADRP on farmland prices in Taiwan. A unique dataset on 97,864 parcels of farmland transacted in the farmland market is used. By estimating the fixed effect and instrumental variable fixed effect model, a negative overall effect of the incidence and the level of ADRP payments on farmland prices is evident. Moreover, the effect is more pronounced among farmland located in urban areas. This finding provides evidence that the negative stigmatized effect dominates the positive capitalization effect of the ADRP payments on farmland values, especially for farmland located in urban areas (JEL Q15, Q18, Q54).