bluuebunny/crossref_metadata_2025 huggingface dataset:
Causal relationship between COVID-19 and myocarditis or pericarditis risk: a bidirectional Mendelian randomization study
Authors: Liu Guihong , Chen Tao , Zhang Xin , Hu Binbin , Shi Huashan
Published: December 2023

Background & aims

Coronavirus disease 2019 (COVID-19) is strongly associated with myocarditis or pericarditis risk in observational studies, however, there are still studies that do not support the above conclusion. Whether the observed association reflects causation needs to be confirmed. We performed a bidirectional Mendelian randomization (MR) study to assess the causal relationship of COVID-19, which was divided into three groups, namely severe COVID-19, hospitalized COVID-19, and COVID-19 infection, measured by myocarditis or pericarditis.

Methods

We extracted summary genome-wide association statistics for the severe COVID-19 (case: 13,769, control: 1,072,442), hospitalized COVID-19 (case: 32,519, control: 2,062,805), COVID-19 infection (case: 122,616, control: 2,475,240), myocarditis (case 1,521, control 191,924), and pericarditis (case 979, control 286,109) among individuals of European ancestry. Independent genetic variants that exhibited a significant association with each phenotype at the genome-wide level of significance were utilized as instrumental variables. Estimation of the causal effect was mainly performed using the random effects inverse-variance weighted method (IVW). Additionally, other tests such as MR-Egger intercept, MR-PRESSO, Cochran's Q-test, “Leave-one-out”, and funnel plots were conducted to assess the extent of pleiotropy and heterogeneity.

Results

Non-associations in the IVW and sensitivity analyses were observed for COVID-19 with myocarditis or pericarditis. Severe COVID-19 was not associated with myocarditis [odds ratio (OR), 1.00; 95% confidence interval (CI), 0.89–1.12; P = 0.99], pericarditis (OR = 0.90, 95% CI, 0.78–1.04, P = 0.17). Similar results can be observed in hospitalized COVID-19, and COVID-19 infection. At the same time, null associations were observed for myocarditis or pericarditis with COVID-19 traits in the reverse direction. The main results are kept stable in the sensitivity analysis.

Conclusion

There is no evidence that COVID-19 is independently and causally associated with myocarditis or pericarditis.
Are ambulatory blood pressure parameters associated more with central adiposity than with total adiposity? Results of the ELSA-Brasil study
Authors: Souza Ângela Maria Natal de , Griep Rosane Harter , Hermsdorff Helen Hermana Miranda , Fonseca Maria de Jesus Mendes da , Juvanhol Leidjaira Lopes
Published: December 2023

Background

Worldwide obesity has a high prevalence, as well as carries a high risk of several chronic diseases, including hypertension. Studies of the association between obesity and ambulatory blood pressure (BP) are scarce and most use only body mass index (BMI) as indicator of adiposity. Thus, we aimed to examine for associations between total and central adiposity and ambulatory BP parameters (BP means and variability, nocturnal dipping and morning surge) among participants in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil).

Methods

This cross-sectional study (2012–2014) used a subsample of participants (n = 812) of ELSA-Brasil who underwent 24-hour ambulatory BP monitoring to assess systolic and diastolic BP (SBP and DBP, respectively) over 24-hour periods and sub-periods. Indicators for total adiposity were BMI and body fat (BF) and, for central adiposity, waist circumference (WC) and waist-to-height ratio (WHR). Associations were tested using crude and adjusted gamma and logistic regression.

Results

Overweight (BMI) and abdominal obesity (WC and WHR) associated positively with mean 24-hour (Coef = 2.71, 3.09 and 4.00, respectively), waking (Coef = 2.87, 3.26 and 4.16, respectively), and sleeping (Coef = 2.30, 2.74 and 3.50, respectively) SBP; mean DBP associated with high WHR in these three periods (Coef = 2.00, 2.10 and 1.68, respectively) and with WC in the waking period (Coef = 1.44). Overweight and abdominal obesity (WC and WHR) were positively associated with SBP variability over 24 h (Coef = 0.53, 0.45 and 0.49, respectively) and in sleep (Coef = 0.80, 0.74 and 0.59, respectively), and with DBP variability in 24 h (Coef = 0.64, 0.73 and 0.58, respectively), wakefulness (Coef = 0.50, 0.52 and 0.52, respectively) and sleep (Coef = 0.53, 0.45 and 0.49); excess BF associated positively with DBP variability over 24 h (Coef = 0.43) and in wakefulness (Coef = 0.38). Lastly, high WHR and excess BF were associated with higher odds of extreme dipping (OR = 1.03 for both), while high WC and WHR associated with higher odds of exacerbated diastolic morning surge (OR = 3.18 and 3.66, respectively).

Conclusion

Indicators of adiposity were associated with the BP means and variability, nocturnal dipping and morning surge, with more substantial results for indicators of central adiposity that the others.
Standardized in vitro bleeding tests in a non-coated novel hybrid prosthesis for frozen elephant trunk demonstrates minimal oozing during full heparinization, supported by clinical data
Authors: Jakob Heinz , Leins Timo , Avci-Adali Meltem , Schlensak Christian , Wendt Daniel , Mehta Yatin , Thielmann Matthias , Görlinger Klaus , Song Suk-Won , Tsagakis Konstantinos
Published: December 2023

Introduction

Recent reports have questioned the blood impermeability of the novel frozen elephant trunk (FET) device E-vita Open NEO© (EO-NEO). Therefore, standardized in vitro bleeding tests using porcine heparinized blood were performed, as well as stress testing on the blood tightness of the collar suture line, to investigate this observation.

Material and methods

EO-NEO prostheses were examined in vitro for blood permeability in three test series. Initially, antegrade perfusion with heparinized porcine blood [activated clotting time (ACT) of 500 s, with a 60 min duration] was performed, followed by ante/retrograde testing via the EO-NEO side port. Testing of the collar suture line under a tension of 10 Newton (N) within a suspension device (blood pressure 120 mmHg, ACT of 560 s, 1 min duration) was carried out with the suture material force fiber white (FFWs) yarn, using standard fixation (5 stitches/cm), FFWh yarn in hemostatic fixation (15 stitches/cm), and flow weave yarn (FWYh).

Results

Blood permeability testing of EO-NEO through the prosthetic lumen or via the side port demonstrated minor leakage without statistical difference between the standard and hemostatic suture lines or suture materials used, or positioning on the crimped or tapered portion (p > 0.05). The specific collar anastomosis testing demonstrated leakage volumes of 140 ml/min for FFWs vs. 16 ml/min for FFWh (p = 0.02), vs. 9 ml/min with the FWYh (p = 0.01).

Conclusion

Different blood leakage tests showed minimal oozing and no difference in blood loss through the fabric and different collar suture lines, but unphysiological pressurized retrograde perfusion of the collar region showed significantly less leakage using FWYh and FFWh, prompting production modification of EO-NEO. Clinical results confirmed low blood loss using this novel FET device.
General and math vocabulary contributions to early numeracy skills in a large population-representative sample
Authors: Bleses Dorthe , Moos Martin , Purpura David J. , Dale Philip S.
Published: December 2023

There are well-documented associations between numeracy development in preschool age children and general and math vocabulary, literacy, and executive functions. However, the studies have largely included small samples of children with parents with either predominantly low or higher socioeconomic background (SES); further, few studies have included measures of all these domains in the same analyses. In this current study, we examined how general vocabulary, math vocabulary, rhyme detection and three measures of executive functions are associated with numeracy development in a population representative sample of 2,931 Danish children 3–5 years (51% male, 89% white). Multi-level regressions controlling for child age and, sex, and in some analyses also parental education and income confirmed that general vocabulary (β = 0.16), math language (β = 0.17), rhyme detection (β = 0.14), attention shifting (β = 0.09), inhibitory control (β = 0.05), and working memory (β = 0.09) each are associated with numeracy after controlling for covariates when estimated in the same model. Analyses of extreme performance (low-high) suggests a much closer and symmetrical connection between math language and numeracy compared to that between general vocabulary and numeracy. Interestingly, family SES is weakly but significantly related to all measures, most strongly for the vocabulary measures, but does not influence the pattern of results from regression analyses. In conclusion, both general vocabulary and mathematics-specific vocabulary contribute substantially to early numeracy skills. Rhyme detection and executive functions are also associated with numeracy skills, but with a lower magnitude.
Review of discrete fracture network characterization for geothermal energy extraction
Authors: Medici Giacomo , Ling Fanlin , Shang Junlong
Published: December 2023

Geothermal reservoirs are highly anisotropic and heterogeneous, and thus require a variety of structural geology, geomechanical, remote sensing, geophysical and hydraulic techniques to inform Discrete Fracture Network flow models. Following the Paris Agreement on reduction of carbon emissions, such reservoirs have received more attention and new techniques that support Discrete Fracture Network models were developed. A comprehensive review is therefore needed to merge innovative and traditional technical approaches into a coherent framework to enhance the extraction of geothermal energy from the deep subsurface. Traditionally, statistics extracted from structural scanlines and unmanned aerial vehicle surveys on analogues represent optimum ways to constrain the length of joints, bedding planes, and faults, thereby generating a model of the network of fractures. Combining borehole images with seismic attributes has also proven to be an excellent approach that supports the stochastic generation of Discrete Fracture Network models by detecting the orientation, density, and dominant trends of the fractures in the reservoirs. However, to move forward to flow modelling, computation of transmissivities from pumping tests, and the determination of hydraulically active fractures allow the computation of the hydraulic aperture in permeable sedimentary rocks. The latter parameter is fundamental to simulating flow in a network of discrete fractures. The mechanical aperture can also be estimated based on the characterization of geomechanical parameters (Poisson’s ratio, and Young’s modulus) in Hot Dry Rocks of igneous-metamorphic origin. Compared with previous review studies, this paper will be the first to describe all the geological and hydro-geophysical techniques that inform Discrete Fracture Network development in geothermal frameworks. We therefore envisage that this paper represents a useful and holistic guide for future projects on preparing DFN models.
A systematic literature review of math interventions across educational settings from early childhood education to high school
Authors: Svane Riikka Pauliina , Willemsen Marinka Marianne , Bleses Dorthe , Krøjgaard Peter , Verner Mette , Nielsen Helena Skyt
Published: December 2023

Early math skills lay the foundation for children’s long-term academic success. An increasing number of randomized controlled math interventions have been carried out across educational settings. The aim of the present systematic review was to identify the distribution of the randomized controlled math interventions conducted between 2001 and 2021 in educational settings across Early Childhood Education (ECE) up to high school among various sample types, and to describe their central features at each educational setting separately. Based on the knowledge gaps exposed through the systematic review, we aimed to discuss where and how future math interventions are still needed in order to optimize all children’s math skill development across educational settings and sample types from early on. A total of n = 75 math interventions meeting the inclusion criteria using the PRISMA-guidelines were identified, of which the majority of them were executed in the elementary school, mostly targeting at-risk children. It is proposed that there is still a large potential of promoting children’s math skills from early on in the ECE settings, utilizing both teachers and parents, among at-risk and non-at-risk samples.
Examining racial and gender biases in teaching evaluations of instructors by students on an online platform in the UAE
Authors: Lamba Nishtha , Kishore Sameer , Khokhlova Olga
Published: December 2023

Introduction

Student evaluations of teachers (SETs) carry significance for academics' career progression, but evidence suggests that these are influenced by students' expectations and biases. Previous research has shown that female lecturers are viewed less favorably compared to male lecturers. Racial bias has also been observed in higher education. For example, few studies administered in the U.S. found that African American lecturers received lower ratings than White lecturers. Current research investigates whether biases based on instructors' gender (male, female) and race (White and South Asian) influence university students' perception of instructors in an online teaching environment in the UAE.

Methods

Using a between group design, 318 participants viewed one of the four videos (male-South Asian, female-South Asian, male-White, female-White) of a virtual instructor teaching social psychology and then responded to teaching evaluation questions.

Results

Factorial ANOVAs were administered. Biases showcased consistent preference for male and South Asian lecturers. Male lecturers were perceived to be treating students with more respect, speaking in a more appropriate manner, displaying more enthusiasm for the subject, and more approachable than female lecturers. South Asian lecturers were perceived to be more sensitive toward students' feelings, displaying more enthusiasm for the subject, and more approachable than White lecturers. Overall, students expressed wanting to study more from male and South Asian lecturers compared to female and White lecturers.

Discussion

Biases emerged in interpersonal variables, such as approachability, sensitivity, enthusiasm for subject, and respect, and not in the domains of knowledge, presentation skills, and stimulating thinking. Findings reinforce that relying on teaching evaluations may not be accurate, and highlight how certain unconscious biases could impact professional growth of academics.
Psychometric properties of the Propensity to Morally Disengage Scale in Peruvian university students: internal structure and association with the dark triad
Authors: Lingán-Huamán Susana K. , Dominguez-Lara Sergio , Serpa-Barrientos Antonio
Published: December 2023

Introduction

Human morality is an important topic because the fact that human beings can determine if a behavior is correct or incorrect, good or bad, shows that they are endowed with moral conscience, but In the Peruvian context, there are no valid and reliable scales to measure moral disengage trend. This study aimed to analyze the psychometric properties of the Propensity to Morally Disengage Scale in Peruvian university students.

Method

The number of university students who participated in the study was 591 (women = 71.7%; Mage = 21.5; SDage = 3.60), and the data analysis was carried out under a factor analysis approach.

Results

The results showed a unidimensional structure in both men and women. As for the invariance results according to sex, there is favorable evidence of configural and weak invariance; however, there is no evidence of strong invariance. Furthermore, the reliability of the construct (coefficient ω) and its scores (coefficient α) reached acceptable dimensions in each group and positive associations with the dark triad (Machiavellianism, psychopathy, and narcissism).

Discussion

The Propensity to Morally Disengage Scale is a unidimensional measurement that shows acceptable psychometric evidence in men and women separately. The theoretical and practical implications are discussed.
Spatiotemporal development of national botanic gardens worldwide and their contributions to plant diversity conservation from 1593 to 2023
Authors: Li Yiyi , Li Shidong , Zhao Guangshuai
Published: December 2023

The national botanic garden is not only a primary means of global ex situ conservation but also a key indicator of biodiversity conservation capacity in a country. To understand the contributions of national botanic gardens from around the world to plant diversity conservation, we generated a long time-series dataset to investigate the spatiotemporal development of national botanic gardens and then explored their functional expansion from 1593 to 2023. The results showed that the development of national botanic gardens was driven by the development demands of human society and could be divided into three stages, i.e., the initial exploration stage of early botanic gardens (1593–1765), the colonial development stage of modern botanic gardens (1765–1945), and the rapid development stage of modern botanic gardens (1945–2023). The first national botanic garden was established in Western Europe, followed by other national botanic gardens being established in the rest of the world. The functions of national botanic gardens evolved from the collection of medicinal and plant resources to multiple purposes, including scientific research, plant diversity conservation, education, and dissemination of knowledge. Contemporary national botanical gardens have played a crucial role in plant diversity conservation and scientific research on the response and adaptation of plant diversity to global change. Future development and management of national botanic gardens will play a crucial role in achieving future targets of the post-2020 global biodiversity framework, which meets the UN Sustainable Development Goals.
Unveiling immune checkpoint regulation: exploring the power of in vivo CRISPR screenings in cancer immunotherapy
Authors: Wang Yuxiang , Khalil Athar , Kamar Amina , Du Mengyan , Dinh Trang , McFarland Christopher , Wang Zhenghe
Published: December 2023

Immune checkpoint inhibitors (ICIs) have revolutionized cancer immunotherapy by reinvigorating antitumor immune responses, but their efficacy remains limited in most patients. To address this challenge and optimize Immune check inhibitor treatment, understanding the underlying molecular intricacies involved is crucial. The emergence of CRISPR-Cas9 technology has empowered researchers to precisely investigate gene function and has introduced transformative shifts in identifying key genes for various physiological and pathological processes. CRISPR screenings, particularly in vivo CRISPR screenings, have become invaluable tools in deciphering molecular networks and signaling pathways governing suppressive immune checkpoint molecules. In this review, we provide a comprehensive overview of in vivo CRISPR screenings in cancer immunotherapy, exploring how this cutting-edge technology has unraveled potential novel therapeutic targets and combination strategies. We delve into the latest findings and advancements, shedding light on immune checkpoint regulation and offering exciting prospects for the development of innovative and effective treatments for cancer patients.
Primary biliary cirrhosis and osteoporosis: a bidirectional two-sample Mendelian randomization study
Authors: Zhao Diqian , Li Guobi , Bai Wenzhe , Teng Jiawen , Yan Bing , Han Cong
Published: December 2023

Background

Observational studies have identified a heightened risk of osteoporosis and fractures in patients with primary biliary cholangitis (PBC). However, conclusive evidence establishing a causal relationship between the two, and a clear mechanism explaining this association, remains elusive.

Methods

We conducted a bidirectional two-sample Mendelian randomization (MR) analysis to investigate the causal relationship between PBC and osteoporosis. This analysis utilized five MR methods: inverse-variance weighted (IVW), MR-Egger, weighted median, weighted mode, and simple mode. Sensitivity analyses were performed, employing various models and testing methods, to assess the impact of heterogeneity and pleiotropy on the results and to confirm their robustness.

Results

A causal relationship between PBC and osteoporosis risk was established through IVW analysis (OR: 1.049, 95%CI: 1.017–1.082, P=0.002). Three other MR analyses corroborated these findings. Conversely, osteoporosis was not found to causally affect PBC risk, as evidenced by IVW analysis (OR: 0.941, 95%CI: 0.783–1.129, P=0.511). Across all MR analyses, no heterogeneity or horizontal pleiotropy was detected among the instrumental variables (IVs). Furthermore, the leave-one-out analysis indicated that no single SNP disproportionately influenced the results, affirming the reliability of the bidirectional MR findings.

Conclusion

This study establishes a positive causal relationship between PBC and the risk of osteoporosis, while no definitive causal link was found from osteoporosis to PBC. These findings offer new insights and guidance for managing bone health in PBC patients.
The role of microRNAs involved in the disorder of blood–brain barrier in the pathogenesis of multiple sclerosis
Authors: Emami Nejad Asieh , Mostafavi Zadeh Seyed Mostafa , Nickho Hamid , Sadoogh Abbasian Ali , Forouzan Azim , Ahmadlou Mojtaba , Nedaeinia Reza , Shaverdi Saham , Manian Mostafa
Published: December 2023

miRNAs are involved in various vital processes, including cell growth, development, apoptosis, cellular differentiation, and pathological cellular activities. Circulating miRNAs can be detected in various body fluids including serum, plasma, saliva, and urine. It is worth mentioning that miRNAs remain stable in the circulation in biological fluids and are released from membrane-bound vesicles called exosomes, which protect them from RNase activity. It has been shown that miRNAs regulate blood-brain barrier integrity by targeting both tight junction and adherens junction molecules and can also influence the expression of inflammatory cytokines. Some recent studies have examined the impact of certain commonly used drugs in Multiple Sclerosis on miRNA levels. In this review, we will focus on the recent findings on the role of miRNAs in multiple sclerosis, including their role in the cause of MS and molecular mechanisms of the disease, utilizing miRNAs as diagnostic and clinical biomarkers, using miRNAs as a therapeutic modality or target for Multiple Sclerosis and drug responses in patients, elucidating their importance as prognosticators of disease progression, and highlighting their potential as a future treatment for MS.
Single-cell RNA sequencing analysis reveals the heterogeneity of IL-10 producing regulatory B cells in lupus-prone mice
Authors: Daamen Andrea R. , Alajoleen Razan M. , Grammer Amrie C. , Luo Xin M. , Lipsky Peter E.
Published: December 2023

Introduction

B cells can have both pathogenic and protective roles in autoimmune diseases, including systemic lupus erythematosus (SLE). Deficiencies in the number or immunosuppressive function of IL-10 producing regulatory B cells (Bregs) can cause exacerbated autoimmune inflammation. However, the exact role of Bregs in lupus pathogenesis has not been elucidated.

Methods

We carried out gene expression analysis by scRNA-seq to characterize differences in splenic Breg subsets and molecular profiles through stages of disease progression in lupus-prone mice. Transcriptome-based changes in Bregs from mice with active disease were confirmed by phenotypic analysis.

Results

We found that a loss of marginal zone (MZ) lineage Bregs, an increase in plasmablast/plasma cell (PB-PC) lineage Bregs, and overall increases in inflammatory gene signatures were characteristic of active disease as compared to Bregs from the pre-disease stage. However, the frequencies of both MZ Bregs and PB-PCs expressing IL-10 were significantly decreased in active-disease mice.

Conclusion

Overall, we have identified changes to the repertoire and transcriptional landscape of Breg subsets associated with active disease that provide insights into the role of Bregs in lupus pathogenesis. These results could inform the design of Breg-targeted therapies and interventions to restore Breg suppressive function in autoimmunity.
Experiences of and support for black women in ecology, evolution, and marine science
Authors: Traylor-Knowles Nikki , Bedi de Silva Anamica , Boyd Anjali D. , Callwood Karlisa A. , Davis Alexandra C. D. , Hall Giselle , Moreno Victoria , Scott Cinda P.
Published: December 2023

Systemic racism and sexism are well documented in ecology, evolution, and marine science. To combat this, institutions are making concerted efforts to recruit more diverse people by focusing on the recruitment of Black people. However, despite these initiatives, white supremacy culture still prevails. The retention of Black people in ecology, evolution, and marine science has not increased in the ways that were hoped for. This is particularly true for Black women, who struggle to find a safe working environment that values their contributions and allows them to openly celebrate their own culture and identity. In this perspective article, we discuss the challenges that Black women face every day, and the needs of Black women to thrive in ecology, evolution, and marine science. We have written this directly to Black women and provide information on not only our challenges, but our stories. However, readers of all identities are welcome to listen and examine their role in perpetuating systemic racism and sexism. Lastly, we discuss support mechanisms for navigating ecology, evolution, and marine science spaces so that Black women can thrive.
Vibroacoustic therapy in the treatment of patients with COVID-19 complicated by respiratory failure: a pilot randomized controlled trial
Authors: Konkayev Aidos , Bekniyazova Assema
Published: December 2023

Introduction

Coronavirus infection is a dangerous airborne disease that can lead to serious lung damage. Data on the effectiveness of low-frequency chest vibrations in the treatment of lung diseases are available; however, not so many of them exist. Vibroacoustic pulmonary therapy is a component of physiotherapy that improves lung perfusion and drainage without requiring active patient participation. This study aimed to increase statistical efficiency through maximizing the relevant information obtained from the clinical data. Calculating the sample size to determine the power of subsequent studies was also necessary.

Research methods

A pilot randomized parallel trial involving 60 patients was conducted. The patients were divided into two equal groups, where they received sessions of vibroacoustic pulmonary therapy using the “VibroLung” device in two modes “acute respiratory distress syndrome (ARDS)” and “Pneumonia,” with identical treatment. The patients were > 18 years old with detected COVID-19 by PCR and grade 2 and 3 lung lesions detected by computer tomography (CT). Blood sampling was performed in the morning at the same time before and after the hardware massage to determine PaO2, PaCO2, and P/F.

Results

As a result of the test, the following data were obtained: on the first day in the group using the “ARDS” mode, PaO2 indicators averaged 65, CI 95% [58.6–73.2] and on average 77.5, CI 95% [69.8–85.2], “before” and “after,” respectively, which indicates improved oxygenation after the procedure. However, in the second group with the “Pneumonia” mode after its use, PaCO2 was higher after the session, on average 48.7, CI 95% [40.8–56.6], whereas before that, the following indicators had, on average 43.6, CI 95% [37.2–50].

Conclusion

Thus, the data obtained yielded ambiguous results, which are the basis for further study in future randomized controlled trials. As the treatment of coronavirus infection has no etiological treatment, even small shifts in the therapy of this category of patients can be significant.

Clinical trial registration

ClinicalTrials.gov, identifier NCT05143372.
Tuberculosis patients with special clinical conditions treated with contezolid: three case reports and a literature review
Authors: Wang Jun , Ma Liping
Published: December 2023

Background

Contezolid is a novel oxazolidinone antibacterial agent, but there have been no reports of any pertinent clinical studies for the treatment of tuberculosis (TB). This was the first report of three TB patients who were successfully treated with contezolid.

Case presentation

Case 1 was TB complicated by myelosuppression syndrome. Case 2 was drug-resistant TB complicated by cirrhosis and anemia. Case 3 was drug-resistant TB complicated by liver transplantation that developed severe anemia after linezolid treatment. Following contezolid therapy, the three patients’ symptoms improved significantly, and no adverse reactions were observed. The chest computed tomography (CT) examination also indicated that the therapeutic effect of this anti-TB regimen was as expected.

Conclusion

Contezolid showed good efficacy and fewer side effects in the treatment of TB. It may be a promising TB treatment.
Long-term efficacy and safety of guselkumab in Chinese patients with moderate-to-severe plaque psoriasis
Authors: Zheng Jianfeng , Chen Wenjuan , Yi Xuemei , Yu Ning , Ding Yangfeng , Gao Yunlu
Published: December 2023

Background

Randomized controlled trials indicated guselkumab, the first anti-interleukin-23 monoclonal antibody, is efficacious in plaque psoriasis. However, guselkumab's performance in real life is scarcely examined, especially in China.

Objectives

This work aimed to assess the long-term effectiveness of guselkumab in actual clinical practice in China.

Methods

A retrospective study was performed for plaque psoriasis cases administered guselkumab in Shanghai Skin Disease Hospital between January 2020 and September 2022.

Results

A total of 37 patients were included (29 men, 78.4%), with a mean follow-up period of 72.3 ± 26.7 weeks (range of 12–108 weeks). At baseline, clinical examination revealed a mean PASI of 12.3 ± 7.1, a mean BSA of 17.1 ± 18.1, and a mean DLQI of 7.7 ± 4.3. Twenty-two (62.9%) and 17 (48.6%) cases achieved PASI 90 and PASI 100 responses at week 28. From weeks 60 to 92, >80% of cases achieved PASI 90 and PASI 100 responses. Regarding safety, no cases of serious AEs were recorded. A total of nine cases (24.3%) had different abnormal results in HBV markers, and two were T-SPOT positive. There was no hepatitis B virus or tuberculosis outbreak in these patients.

Conclusion

This real-life study confirmed the long-term efficacy and safety of guselkumab in daily clinical practice.
Various green manure-fertilizer combinations affect the soil microbial community and function in immature red soil
Authors: Xu Jing , Si Linlin , Zhang Xian , Cao Kai , Wang Jianhong
Published: December 2023

Green manure application is a common practice to improve soil fertility in China. However, the impact of different green manure-fertilizer combinations on the soil microbial communities in the low-fertility immature red soil in southern China remains unclear. In this study, we conducted a pot experiment using two common green manure crops, ryegrass (Lolium perenne L.) and Chinese milk vetch (Astragalus sinicus L.), along with a fallow treatment. We also considered three combined fertilizer management strategies, including mineral, humic acid, and organic manure fertilizers. We evaluated the soil microbial biomass, activity, communities, functional prediction and their correlation with soil properties during green manure growth and incorporation periods, to assess the potential alterations caused by different green manure and fertilizer combinations. Our findings indicate that green manure application, particularly in combination with organic fertilizers, increased the alpha diversity of the soil bacterial community, while the opposite trend was observed in the fungal community. The application of green manure altered the soil microbial communities during both growth and incorporation periods, especially the taxa that participate in carbon, nitrogen and sulfur cycles. Notably, ryegrass significantly increased the relative abundance of bacterial phylum Firmicutes and fungal phylum Ascomycota, whereas Chinese milk vetch significantly stimulated the bacterial phylum Acidobacteria and fungal phylum Glomeromycota. Compared with fallow treatments, green manure application significantly increased the soil pH by 4.1%–12.4%, and microbial biomass carbon by 29.8%–72.9%, regardless of the types of combined fertilizer. Additionally, the application of green manure resulted in a 35.6%–142.6% increase in urease activity and a 65.9%–172.9% increase in β-glucosidase activity compared to fallow treatments, while led to a 22.5%–55.6% decrease in catalase activity. Further analysis revealed that the changes in both bacterial and fungal communities positively correlated with soil pH, soil organic matter, total nitrogen and alkali hydrolyzed nitrogen contents. Moreover, the relationship between the soil microbial community and soil enzyme activities was regulated by the specific green manure species. In conclusion, our results provide insight into the effects of different green manure-fertilizer combinations on soil microorganisms and their underlying mechanisms in improving soil fertility in the low-fertility immature red soil.
Identification and characterization of two closely related virga-like viruses latently infecting rubber trees (Hevea brasiliensis)
Authors: Zhao Ruibai , Su Xiaoqi , Yu Fengjuan , Liu Zhu , Huang Xi
Published: December 2023

A novel virga-like virus, provisionally named Rubber tree latent virus 2 (RTLV2), was identified from rubber tree (Hevea brasiliensis). It is a close relative of the previously reported Rubber tree latent virus 1 (RTLV1). The complete genomes of RTLV1 and RTLV2 were sequenced and comparatively analyzed in terms of genome organization, putative gene products and phylogenetic relationship. Both RTLV1 and RTLV2 have positive-sense single-stranded RNA genomes that encode seven open reading frames (ORFs), forming a similar genomic layout. In phylogenetic analyses based on replicase and coat protein amino acid sequences, RTLV1 and RTLV2 were clustered with unclassified virga-like viruses. They are distinct from currently recognized plant virus families. RTLV1 and RTLV2 can be distinguished from members of Virgaviridae by the presence of a putative coat protein duplex and a poly(A) tail at the 3′-terminus. The authenticity of RTLV1 and RTLV2 as infectious viruses was confirmed through field investigations and transmissibility assays. In conclusion, RTLV1 and RTLV2 represent a novel plant virus group that does not readily fit into current virus families.
Investigation of the potential of Brevibacillus spp. for the biosynthesis of nonribosomally produced bioactive compounds by combination of genome mining with MALDI-TOF mass spectrometry
Authors: Jähne Jennifer , Herfort Stefanie , Doellinger Joerg , Lasch Peter , Tam Le Thi Thanh , Borriss Rainer , Vater Joachim
Published: December 2023

The biosynthetic potential of 11 Brevibacillus spp. strains was investigated by combination of genome mining with mass spectrometric analysis using MALDI-TOF mass spectrometry. These endophytic, plant associated Brevibacillus strains were isolated from crop plants, such as coffee and black pepper, in Vietnam. Draft genomes of these strains were available. They were classified (a) by comparison with type strains and a collection of genome-sequenced Brevibacillus spp. deposited in the NCBI data base as well as (b) by construction of a phylogenetic tree from the core sequences of publicly available genomes of Brevibacillus strains. They were identified as Brevibacillus brevis (1 strain); parabrevis (2 strains); porteri (3 strains); and 5 novel Brevibacillus genomospecies. Our work was specifically focused on the detection and characterization of nonribosomal peptides produced by these strains. Structural characterization of these compounds was performed by LIFT-MALDI-TOF/TOF mass spectrometric sequence analysis. The highlights of our work were the demonstration of the tyrocidines, a well-known family of cyclodecapeptides of great structural variability, as the main products of all investigated strains and the identification of a novel class of pentapeptides produced by B. brevis; B. schisleri; and B. porteri which we designate as brevipentins. Our biosynthetic studies demonstrate that knowledge of their biosynthetic capacity can efficiently assist classification of Brevibacillus species.
Seroepidemiological investigation of Getah virus in the China-Myanmar border area from 2022-2023
Authors: Liu Hao , Hu Jin , Li Li-Xia , Lu Zi-Shuo , Sun Xiu-Tao , Lu Hui-Jun , Jin Ning-Yi , Zhang Lei , Zhang Li-Na
Published: December 2023

Getah Virus (GETV) is an RNA virus that is transmitted by mosquitoes and can cause disease or death in a variety of vertebrates. Its prevalence is increasingly severe in Asia. This study conducted a GETV epidemiological investigation on 1,300 bovine sera collected in the Honghe Prefecture of Yunnan Province on the China-Myanmar border from 2022 to 2023. The positive rate of GETV antibodies in bovine serum in Honghe Prefecture was determined to be 20.25% through indirect Enzyme-linked immunosorbent test (ELISA) methods. Using Real-time PCR methods to detect GETV RNA in bovine serum, the positive rate was 0.23% (3/1300), and viral nucleic acids were only detected in three bovine sera in Jianshui area in 2022. The YN2305 strain was successfully isolated from mouse neuroblastoma (N2a) cells and the complete gene sequence was obtained. All the above results indicate the existence of GETV infection in cattle in Honghe Prefecture, Yunnan Province. Homology and genetic evolution analysis found that the isolated strain has a high homology with the JL1808 strain isolated from cattle in 2018, with a nucleotide identity of 100%, and a nucleotide identity of 99.8% with the SD17-09 strain isolated from foxes in 2017. Compared with the nucleotides of 44 virus strains published in Genbank, YN2305 has multiple nucleotide site mutations in the structural gene E2 and non-structural gene NS. The nucleotide and amino acid identity of the E2 gene are 94.2−100% and 96.4−100%, respectively. Genetic evolution analysis found that this virus strain is most closely related to the bovine origin JL1808, and it is in gene group III with HuN1, Kochi-01, SD17-09, MI-110-C1, and MI-110-C2 strains that causes significant clinical symptoms in Chinese pig, fox and horse populations, belonging to the same evolutionary branch. This study determined the infection rate, genotype, and main prevalence areas of GETV in bovine sera in the China-Myanmar border area. Therefore, the epidemiological investigation of GETV infection in multiple animal hosts should be further expanded, and research on its pathogenicity and vectors should be carried out.
Clinical research with probiotics as an indicator of global valorization since the year 2000
Authors: Wiegers Cato , van Beek Eveline H. T. , Larsen Olaf F. A.
Published: December 2023

Probiotics are becoming increasingly popular due to their potential health benefits. With this rise in popularity and demand as indicated by ever-growing market prospects, it seems evident that innovation and valorization are on the rise as well. However, an increasing body of literature shows that innovation is stagnating, which may be detrimental to the exploitation of the benefits of probiotics, for example the development of alternative therapies to manage the increasing prevalence of metabolic and autoimmune disorders. To this end, this study investigated global clinical trials that have been executed since the year 2000 as a first indicator of the status of probiotic valorization. The cumulative number of clinical trials has indeed increased significantly from 0 at the start of the century up to 2,517 registered trials in 2023. However, in Asia, Europe, and North America, the continents with the highest numbers of clinical trials, stagnating or declining trends have been found. In these locations, most clinical trials were funded by non-industry sponsors and targeting probiotic supplements or undefined products. Considering the overall stagnation in clinical trials and viewing these trends in the context of developments in local markets and regulations, the global valorization of probiotics appears to slow down. This could impact the transition from academic research to the development of products that are beneficial and accessible for consumers, either to maintain a healthy lifestyle or to treat medical conditions.
Tau PET burden in Brodmann areas 35 and 36 is associated with individual differences in cognition in non-demented older adults
Authors: Rani Nisha , Alm Kylie H. , Corona-Long Caitlin A. , Speck Caroline L. , Soldan Anja , Pettigrew Corinne , Zhu Yuxin , Albert Marilyn , Bakker Arnold
Published: December 2023

Introduction

The accumulation of neurofibrillary tau tangles, a neuropathological hallmark of Alzheimer’s disease (AD), occurs in medial temporal lobe (MTL) regions early in the disease process, with some of the earliest deposits localized to subregions of the entorhinal cortex. Although functional specialization of entorhinal cortex subregions has been reported, few studies have considered functional associations with localized tau accumulation.

Methods

In this study, stepwise linear regressions were used to examine the contributions of regional tau burden in specific MTL subregions, as measured by 18F-MK6240 PET, to individual variability in cognition. Dependent measures of interest included the Clinical Dementia Rating Sum of Boxes (CDR-SB), Mini Mental State Examination (MMSE), and composite scores of delayed episodic memory and language. Other model variables included age, sex, education, APOE4 status, and global amyloid burden, indexed by 11C-PiB.

Results

Tau burden in right Brodmann area 35 (BA35), left and right Brodmann area 36 (BA36), and age each uniquely contributed to the proportion of explained variance in CDR-SB scores, while right BA36 and age were also significant predictors of MMSE scores, and right BA36 was significantly associated with delayed episodic memory performance. Tau burden in both left and right BA36, along with education, uniquely contributed to the proportion of explained variance in language composite scores. Importantly, the addition of more inclusive ROIs, encompassing less granular segmentation of the entorhinal cortex, did not significantly contribute to explained variance in cognition across any of the models.

Discussion

These findings suggest that the ability to quantify tau burden in more refined MTL subregions may better account for individual differences in cognition, which may improve the identification of non-demented older adults who are on a trajectory of decline due to AD.
Advances in the study of Müller glia reprogramming in mammals
Authors: Guo Yi-Ming , Jiang Xinyi , Min Jie , Huang Juan , Huang Xiu-Feng , Ye Lu
Published: December 2023

Müller cells play an integral role in the development, maintenance, and photopic signal transmission of the retina. While lower vertebrate Müller cells can differentiate into various types of retinal neurons to support retinal repair following damage, there is limited neurogenic potential of mammalian Müller cells. Therefore, it is of great interest to harness the neurogenic potential of mammalian Müller cells to achieve self-repair of the retina. While multiple studies have endeavored to induce neuronal differentiation and proliferation of mammalian Müller cells under defined conditions, the efficiency and feasibility of these methods often fall short, rendering them inadequate for the requisites of retinal repair. As the mechanisms and methodologies of Müller cell reprogramming have been extensively explored, a summary of the reprogramming process of unlocking the neurogenic potential of Müller cells can provide insight into Müller cell fate development and facilitate their therapeutic use in retinal repair. In this review, we comprehensively summarize the progress in reprogramming mammalian Müller cells and discuss strategies for optimizing methods and enhancing efficiency based on the mechanisms of fate regulation.
Subcortical functional connectivity and its association with walking performance following deployment related mild TBI
Authors: Newsome Mary R. , Martindale Sarah L. , Davenport Nicholas , Dennis Emily L. , Diaz Marlene , Esopenko Carrie , Hodges Cooper , Jackson George R. , Liu Qisheng , Kenney Kimbra , Mayer Andrew R. , Rowland Jared A. , Scheibel Randall S. , Steinberg Joel L. , Taylor Brian A. , Tate David F. , Werner J. Kent , Walker William C. , Wilde Elisabeth A.
Published: December 2023

Introduction

The relation between traumatic brain injury (TBI), its acute and chronic symptoms, and the potential for remote neurodegenerative disease is a priority for military research. Structural and functional connectivity (FC) of the basal ganglia, involved in motor tasks such as walking, are altered in some samples of Service Members and Veterans with TBI, but any behavioral implications are unclear and could further depend on the context in which the TBI occurred.

Methods

In this study, FC from caudate and pallidum seeds was measured in Service Members and Veterans with a history of mild TBI that occurred during combat deployment, Service Members and Veterans whose mild TBI occurred outside of deployment, and Service Members and Veterans who had no lifetime history of TBI.

Results

FC patterns differed for the two contextual types of mild TBI. Service Members and Veterans with deployment-related mild TBI demonstrated increased FC between the right caudate and lateral occipital regions relative to both the non-deployment mild TBI and TBI-negative groups. When evaluating the association between FC from the caudate and gait, the non-deployment mild TBI group showed a significant positive relationship between walking time and FC with the frontal pole, implicated in navigational planning, whereas the deployment-related mild TBI group trended towards a greater negative association between walking time and FC within the occipital lobes, associated with visuo-spatial processing during navigation.

Discussion

These findings have implications for elucidating subtle motor disruption in Service Members and Veterans with deployment-related mild TBI. Possible implications for future walking performance are discussed.