bluuebunny/crossref_metadata_2025 huggingface dataset:
HCV Treatment Outcomes in PWID: Impact of Addiction History on SVR12
Authors: Milošević Ivana , Beronja Branko , Filipović Ana , Mitrović Nikola , Simić Jelena , Knežević Nataša , Ranin Jovana , Todorović Nevena , Stevanović Olja , Radovanović-Spurnić Aleksandra , Katanić Nataša , Hristović Dejan , Nikolić Nataša
Published: December 2024

People who inject drugs (PWIDs) experience high rates of hepatitis C virus (HCV) infection, primarily due to needle sharing and limited healthcare access, resulting in a disproportionate disease burden within this population. This prospective study evaluated treatment outcomes in 432 adult patients with chronic hepatitis C (CHC) treated with direct-acting antivirals (DAAs) at the University Clinical Center of Serbia. Patients were categorized into two groups based on a history of drug addiction: PWIDs (163, 37.7%) and non-PWIDs (269, 62.3%). The PWID group was further categorized into subpopulations of problematic PWIDs (39, 23.9%), ex-PWIDs (124, 76.1%), and PWIDs on OST (96, 58.9%). The PWID group demonstrated significantly lower treatment adherence, with an intention-to-treat (ITT) rate of 82.8%, compared to 96.3% in the control group (p < 0.001). In contrast, no significant differences were observed in per-protocol (PP) outcomes between the two groups. Additionally, PWIDs were significantly younger (p < 0.001) and had higher rates of psychiatric disorders (p < 0.001), alcohol abuse (p < 0.001), and HCV genotype 1a (p < 0.001). Advanced fibrosis was predictor of PP treatment failure among PWIDs, while mood disorders and alcohol use disorder were associated with interruptions before the scheduled completion time. For non-PWIDs, older age and advanced fibrosis emerged as key predictors of PP treatment failure. The loss to follow-up was most commonly observed in the problematic PWID subgroup (p = 0.001). These findings highlight the importance of addressing barriers in PWIDs through integrated care strategies that concurrently manage addiction and HCV.
Yingde–Guangning Granitic Plutons Record Complex Tectonic Evolution During Paleozoic-Mesozoic: Implications for Gold Exploration in Western Guangdong, South China
Authors: Wang Buqing , Li Huan , Sun Zhihao , Quan Wei , Huang Yuxuan , Faisal Mohamed
Published: December 2024

Western Guangdong, a part of the South China Block, has a complex geological history characterized by significant magmatic, metamorphic, and tectonic activities. This dynamic geological past, particularly during the Mesozoic era, created favorable conditions for the formation of various mineral deposits, including Au, Ag, Cu, and Pb. This makes the region a key area for precious metal resources in China. Despite extensive metallogenic studies, detailed structural information for western Guangdong remains insufficient, highlighting the need for further investigation. Thus, effective delineation of deformation periods is crucial for revealing geodynamic history and understanding regional tectonic activities, which are extremely important for guiding mineral exploration. This work focuses on the outcrops of granitic plutons in the Yingde–Guangning area of western Guangdong to establish the structure–tectonic setting. The tectonic events likely shaped the widespread Paleozoic–Mesozoic granitic bodies, which record extensive information on regional tectonic evolution. To achieve the primary objective, systematic identification and kinematic analysis of the various stages of structural traces, such as foliations and joints, have been conducted. This research proposes, for the first time, that the western Guangdong area underwent four distinct tectonic stages: (1) Early Paleozoic NW-SE compression phase; (2) Triassic NE-SW compressional stress; (3) Jurassic NW-SE compressional force; and (4) Cretaceous NW-SE extension stage. In metallogenic terms, the NW-SE trending auriferous veins of the Yingde–Guangning region were mostly formed during the Triassic NE-SW compression stage, whereas the NE-SW trending vein-type gold mineralization developed during the tectonic regime transformation from Jurassic NW-SE compression to Cretaceous NW-SE extension. This research emphasizes that systematic tectonic geological studies of regional granites can effectively guide mineral prospecting.
A Novel Organic–Inorganic-Nanocomposite-Based Reduced Graphene Oxide as an Efficient Nanosensor for NO2 Detection
Authors: Khaleghiabbasabadi Masoud , Taghavian Hadi , Gholami Pooya , Khodabakhshi Saeed , Gheibi Mohammad , Wacławek Stanisław , Černík Miroslav , Silvestri Daniele , Raczak Klaudia Barbara , Moezzi Reza
Published: December 2024

There are three components to every environmental protection system: monitoring, estimation, and control. One of the main toxic gases with considerable effects on human health is NO2, which is released into the atmosphere by industrial activities and the transportation network. In the present research, a NO2 sensor is designed based on Fe3O4 piperidine-4-sulfonic acid grafted onto a reduced graphene oxide Fe3O4@rGO-N-(piperidine-4-SO3H) nanocomposite, due to the highly efficient detection of pollution in the air. In the first phase of the present study, the nanocomposite synthesis is performed in four steps. Afterward, the novel fabricated nanosensor is characterized through energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), Raman, surface area analysis, and field emission scanning electron microscopy (FE-SEM). To determine the optimal condition for sensor performance, graphene-based nanosensors are prepared with various weight percentages (wt%) of rGO-N-(piperidine-4-SO3H) (1 wt%, 5 wt%, 10 wt%, and 15 wt%). During the experimental process, the performance of the sensors, in terms of the sensitivity and response time, is investigated at different NO2 concentrations, between 2.5 and 50 ppm. The outputs of this study demonstrate that the synthesized nanosensor has the best efficiency at more than a 5 ppm contamination concentration and with at least 15 wt% of rGO-N-(piperidine-4-SO3H).
Luminescent Nanocrystal Probes for Monitoring Temperature and Thermal Energy Dissipation of Electrical Microcircuit
Authors: Jankowski Dawid , Wiwatowski Kamil , Żebrowski Michał , Pilch-Wróbel Aleksandra , Bednarkiewicz Artur , Maćkowski Sebastian , Piątkowski Dawid
Published: December 2024

In this work, we present an experimental approach for monitoring the temperature of submicrometric, real-time operating electrical circuits using luminescence thermometry. For this purpose, we utilized lanthanide-doped up-converting nanocrystals as nanoscale temperature probes, which, combined with a highly sensitive confocal photoluminescence microscope, enabled temperature monitoring with spatial resolution limited only by the diffraction of light. To validate our concept, we constructed a simple model of an electrical microcircuit based on a single silver nanowire with a diameter of approximately 100 nm and a length of about 50 µm, whose temperature increase was induced by electric current flow. By driving electric current only along one half of the nanowire, we created a dual-function microstructure, where one section is a resistive heater, while the other operates as a radiator. Such a combination realistically reflects the electronic circuit and its thermal behavior. We demonstrated that nanocrystals distributed around this circuit allow for remote temperature readout and enable precise monitoring of the thermal energy propagation and heat dissipation processes, which are crucial for designing and developing highly integrated electronic on-chip devices.
Estimating Nitrogen Uptake Efficiency of Mango Varieties from Foliar KNO3 Application Using a 15N Tracer Technique
Authors: Asis Constancio A. , Tilbrook Joanne , Anson Dallas , Niscioli Alan , Bristow Mila , Friedl Johannes , Rowlings David
Published: December 2024

Commercial mango growers commonly spray potassium nitrate (KNO3) solution to enhance flowering and fruit quality, yet there is limited information on the uptake efficiency of nitrogen (N) by mango cultivars through leaf cuticles. The study aimed to assess N uptake efficiency (NUpE) from foliar application of KNO3 solution and compare NUpE among mango varieties. Mango cultivars were ‘Kensington Pride’ (‘KP’), ‘B74’ (‘Calypso®’), and ‘NMBP 1201’ (‘AhHa!®’), ‘NMBP 1243’ (‘Yess!®’), and ‘NMBP 4069’ (‘Now®’) grafted onto ‘KP’ seedlings. Leaves of six-month-old seedlings were dipped in 15N-enriched KNO3 solution and analyzed for total N and 15N contents. A significant correlation was observed between the leaf area and the amount of solution retained after dipping the leaves in the KNO3 solution. Moreover, leaves treated with the KNO3 solution had higher 15N levels than the natural 15N abundance, indicating successful N uptake from the KNO3 solution. The NUpE ranged from 27% to 44% and varied with variety. Cultivar ‘NMBP 4069’ had the highest NUE (44%) which was comparable with that of ‘B74’ (40%). ‘NMBP 1201’ showed the lowest (27%) NUpE which was comparable with that of ‘NMBP 1243’ (30%) and ‘KP’ (33%). These data on 15N uptake through the mango leaf cuticle demonstrates the effectiveness of foliar application as a method of supplying N to mango trees, highlighting important varietal differences in foliar 15N uptake efficiency. Considering these differences in NUpE among mango varieties will help in making informed decisions about cultivar selection and N management strategies for sustainable mango production.
Effects of Nutritional Support with a Leucine-Enriched Essential Amino Acid Supplement on Body Composition, Muscle Strength, and Physical Function in Stroke Patients Undergoing Rehabilitation
Authors: Nakagawa Naoki , Koyama Satoshi , Maruyama Keisuke , Maruyama Jun-Ichi , Hasebe Naoyuki
Published: December 2024

Background/Objectives: Dietary protein intake can potentially influence renal function. This study aimed to elucidate the association between dietary protein supplementation and a decrease in the estimated glomerular filtration rate (eGFR) in Japanese stroke patients undergoing rehabilitation. Methods: From July 2017 to June 2021, 60 patients undergoing post-stroke rehabilitation were randomly assigned to a rehabilitation alone or rehabilitation nutrition group, which received 120 g Reha-Time Jelly® after each session. Both groups were followed up for 3 months. Serum nutritional markers (prealbumin and retinol-binding protein), muscle strength, body composition, renal function markers (eGFR based on creatinine [eGFR-Cr] and cystatin C [eGFR-Cys]), urinary protein-to-creatinine ratio (UPCR), and motor function (walking speed, 2-min walk distance, and chair stand test) were assessed at baseline and post-intervention. Results: Of the 60 participants (mean age: 70.2 ± 10.0 years), 39 were men (65.0%) and 19 (31.7%) had chronic kidney disease. Initial eGFR-Cr and eGFR-Cys values were 70.5 ± 17.2 and 66.6 ± 14.8 mL/min/1.73 m2, respectively. After the intervention, the rehabilitation nutrition group demonstrated a significantly greater increase in body mass index (BMI) and a smaller decrease in bone mineral content than the rehabilitation alone group. However, no significant between-group differences were noted in serum marker levels or motor function, including grip strength and knee extensor strength, on the paralyzed and non-paralyzed sides. The change in chair stand test performance indicated a trend toward improvement in the rehabilitation nutrition group. No significant differences were observed in the changes in renal function. Conclusions: A 3-month nutritional supplementation intervention may help increase BMI, preserve bone mineral content, and support physical activity levels in patients undergoing post-stroke rehabilitation without negatively affecting renal function.
Racial–Ethnic Disparities of Obesity Require Community Context-Specific Biomedical Research for Native Hawaiians and Other Pacific Islanders
Authors: Wells Riley K. , Torres Amada , Mau Marjorie K. , Maunakea Alika K.
Published: December 2024

Compared to the general population of Hawai‘i, Native Hawaiians and Other Pacific Islanders (NHPI) shoulder a disproportionately high risk for obesity-related cardiometabolic disorders, such as type 2 diabetes and cardiovascular disease. The gut microbiome is an area of rapid research interest for its role in regulating adjacent metabolic pathways, offering novel opportunities to better understand the etiology of these health disparities. Obesity and the gut microbiome are influenced by regional, racial–ethnic, and community-specific factors, limiting the generalizability of current literature for understudied populations. Additionally, anthropometric and directly measured obesity indices are variably predictive of adiposity and metabolic health risk in this diverse population. Thus, further NHPI-inclusive research is required to adequately characterize community-specific factors in the context of obesity-related disease etiology. Culturally responsible research ethics and scientific communication are crucial to conducting such research, especially among indigenous and understudied populations. In this review, we explore these limitations in current literature, emphasizing the urgent need for NHPI-inclusive research to assess community-specific factors accurately. Such accuracy in Indigenous health research may ensure that findings relevant to individual or public health recommendations and/or policies are meaningful to the communities such research aims to serve.
Higher Adherence to the EAT-Lancet Diets After a Lifestyle Intervention in a Pediatric Population with Abdominal Obesity
Authors: Ojeda-Rodríguez Ana , Paula-Buestan Gabriela , Zazpe Itziar , Azcona-Sanjulian Maria Cristina , Martí del Moral Amelia
Published: December 2024

Background/Objectives: The rising prevalence of pediatric obesity highlights the urgent need for effective lifestyle interventions that improve diet quality, in line with global health objectives. Tackling obesity through planetarian dietary practices not only enhances individual health but also mitigates the environmental impact of food systems. The EAT-Lancet Commission’s plant-based dietary recommendations underscore the dual benefit of promoting human health while supporting environmental sustainability. This study aims to assess changes in adherence to a planetarian diet, measured through planetary environmental impact indices, following a lifestyle intervention in a pediatric population. Methods: In this randomized controlled trial, 107 participants with abdominal obesity were assigned to either a usual care group or an intensive intervention group, the latter following a moderately hypocaloric Mediterranean diet combined with nutritional education. Adherence to the EAT-Lancet diet was evaluated using both the EAT-Lancet Diet Score and the EAT-Lancet Diet Index. Results: The intensive lifestyle intervention significantly improved adherence to the EAT-Lancet diet, leading to notable reductions in body mass index (BMI), weight, and waist circumference, alongside improvements in both anthropometric and clinical outcomes. Conclusions: This study demonstrates that intensive lifestyle interventions in children and adolescents with abdominal obesity can reduce BMI-SDS (BMI-standard deviation score) and improve adherence to planetarian dietary patterns, leading to enhanced health outcomes. Further research is needed to evaluate the long-term effects of such interventions and to determine their broader applicability across diverse pediatric populations.
Dietary N-6 Polyunsaturated Fatty Acid Intake and Brain Health in Middle-Aged and Elderly Adults
Authors: Gu Jiawei , Bao Yujia , Li Yongxuan , Hua Li , Deng Xiaobei , Zhang Yuzheng , Zhu Xiaojun , Ran Jinjun
Published: December 2024

Background: Dietary intake of polyunsaturated fatty acids (PUFA) plays a significant role in the onset and progression of neurodegenerative diseases. Since the neuroprotective effects of n-3 PUFA have been widely validated, the role of n-6 PUFA remains debated, with their underlying mechanisms still not fully understood. Methods: In this study, 169,295 participants from the UK Biobank were included to analyze the associations between dietary n-6 PUFA intake and neurodegenerative diseases using Cox regression models with full adjustments for potential confounders. In addition, multiple linear regression models were utilized to estimate the impact of n-6 PUFA intake on brain imaging phenotypes. Results: Results indicated that low dietary n-6 PUFA intake was associated with increased risks of incident dementia (hazard ratio [95% confidence interval] = 1.30 [1.13, 1.49]), Parkinson’s disease (1.42 [1.16, 1.74]), and multiple sclerosis (1.65 [1.03, 2.65]). Moreover, the low intake was linked to diminished volumes of various brain structures, including the hippocampus (β [95% confidence interval] = −0.061 [−0.098, −0.025]), thalamus (−0.071 [−0.105, −0.037]), and others. White matter integrity was also found to be compromised in individuals with low n-6 PUFA intake. Conclusions: These findings enhanced our understanding of how dietary n-6 PUFA intake might affect neurological health, thereby providing epidemiological evidence for future clinical and public health interventions.
Association of Metabolic Diseases and Moderate Fat Intake with Myocardial Infarction Risk
Authors: Zhou Junyu , Liu Meiling , Park Sunmin
Published: December 2024

Background: Myocardial infarction (MI) can range from mild to severe cardiovascular events and typically develops through complex interactions between genetic and lifestyle factors. Objectives: We aimed to understand the genetic predisposition associated with MI through genetic correlation, colocalization analysis, and cells’ gene expression values to develop more effective prevention and treatment strategies to reduce its burden. Methods: A polygenic risk score (PRS) was employed to estimate the genetic risk for MI and to analyze the dietary interactions with PRS that affect MI risk in adults over 45 years (n = 58,701). Genetic correlation (rg) between MI and metabolic syndrome-related traits was estimated with linkage disequilibrium score regression. Single-cell RNA sequencing (scRNA-seq) analysis was performed to investigate cellular heterogeneity in MI-associated genes. Results: Ten significant genetic variants associated with MI risk were related to cardiac, immune, and brain functions. A high PRS was associated with a threefold increase in MI risk (OR: 3.074, 95% CI: 2.354–4.014, p < 0.001). This increased the risk of MI plus obesity, hyperglycemia, dyslipidemia, and hypertension by about twofold after adjusting for MI-related covariates (p < 0.001). The PRS interacted with moderate fat intake (>15 energy percent), alcohol consumption (<30 g/day), and non-smoking, reducing MI risk in participants with a high PRS. MI was negatively correlated with the consumption of olive oil, sesame oil, and perilla oil used for cooking (rg = −0.364). MI risk was associated with storkhead box 1 (STOX1) and vacuolar protein sorting-associated protein 26A (VPS26A) in atrial and ventricular cardiomyocytes and fibroblasts. Conclusions: This study identified novel genetic variants and gene expression patterns associated with MI risk, influenced by their interaction with fat and alcohol intake, and smoking status. Our findings provide insights for developing personalized prevention and treatment strategies targeting this complex clinical presentation of MI.
Examining Associations Between Fasting Behavior, Orthorexia Nervosa, and Eating Disorders
Authors: Domaszewski Przemysław , Rogowska Aleksandra M. , Żylak Kaja
Published: December 2024

Background/Objectives: Fasting, orthorexia nervosa, and eating disorders are increasingly prevalent and interconnected. Understanding their relationship is essential for identifying potential risks and developing effective prevention and intervention strategies. This study investigated these associations to enhance our knowledge of their interplay and implications for mental health. Methods: A cross-sectional online survey was conducted in Poland in 2023. A sample of 214 participants aged 16 to 65 (M = 27.95, SD = 9.44) participated in this study. Fasting behavior was the predictor (independent) variable, orthorexia nervosa (measured using the Authorized Bratman Orthorexia Self-Test) was the mediator, and an eating disorder was the dependent variable (assessed using the Eating Attitude Test). Results: The Mann–Whitney U-test indicated that the fasting group scored higher in orthorexia and eating disorder symptoms than the non-fasting sample. Positive associations emerged between the fasting, orthorexia, and eating disorder scales. Linear regression analysis identified significant predictors of eating disorder symptoms, such as age, fasting, dieting, overweight status, and orthorexia. A path analysis revealed that fasting affected eating disorders directly and indirectly through orthorexia. Conclusions: This study identified fasting as a risk factor for orthorexia and other eating disorders, with orthorexia fully mediating the fasting–eating disorder relationship. Clinicians should consider both fasting and orthorexia when assessing patients at risk for eating disorders. This paper also proposes possible intervention and treatment strategies for affected individuals.
The Effect of Quercetin on Non-Alcoholic Fatty Liver Disease (NAFLD) and the Role of Beclin1, P62, and LC3: An Experimental Study
Authors: Katsaros Ioannis , Sotiropoulou Maria , Vailas Michail , Papachristou Fotini , Papakyriakopoulou Paraskevi , Grigoriou Marirena , Kostomitsopoulos Nikolaos , Giatromanolaki Alexandra , Valsami Georgia , Tsaroucha Alexandra , Schizas Dimitrios
Published: December 2024

Background/Objectives: Non-alcoholic fatty liver disease (NAFLD) is a major metabolic disorder with no established pharmacotherapy. Quercetin, a polyphenolic flavonoid, demonstrates potential hepatoprotective effects but has limited bioavailability. This study evaluates the impact of quercetin on NAFLD and assesses the roles of autophagy-related proteins in disease progression. Methods: Forty-seven male C57BL/6J mice were fed a high-fat diet (HFD) for 12 weeks to induce NAFLD, followed by quercetin treatment for 4 weeks. Mice were divided into baseline, control, and two quercetin groups, receiving low (10 mg/kg) and high (50 mg/kg) doses. Liver histology was scored using the NAFLD Activity Score (NAS). Immunohistochemistry and immunoblotting were performed to analyze autophagy markers. Results: Quercetin-treated groups showed significant reductions in NAS compared to controls (p = 0.011), mainly in steatosis and steatohepatitis. Immunohistochemistry indicated increased expression of autophagy markers LCA and p62 in quercetin groups. Western blot analysis revealed significant elevations in LC3A in the treated groups, suggesting improved autophagic activity and lipid degradation. Conclusions: Quercetin effectively reduces NAFLD severity and modulates autophagy-related proteins. These findings suggest that quercetin enhances autophagic flux, supporting its therapeutic potential for NAFLD. Additional research is needed to clarify the molecular mechanisms of quercetin and to determine the optimal dosing for clinical application.
Knowledge and Practices Regarding Deep Venous Thrombosis (DVT) Prevention Among Nurses in Jeddah, Saudi Arabia
Authors: Alharazi Ruba M. , Alqahtani Raiannah H. , Alanazi Rahaf A. , Alharbi Walaa , Alshenen Shmokh M. , Alhofaian Aisha , Tunsi Afnan , Sharif Loujain
Published: December 2024

Background/Objectives: Deep venous thrombosis (DVT), the formation of a blood clot within a large vein, is one of the most common problems among hospitalized patients. The annual prevalence of DVT is 48 per 1,000,000. Nurses’ knowledge significantly affects compliance with VTE risk assessment and prevention. This study aimed to assess the knowledge and practices regarding deep venous thrombosis prevention among nurses in Ministry of Health hospitals and King Abdulaziz University Hospital (KAUH), Jeddah, Saudi Arabia. Methods: This study was conducted in Jeddah using a quantitative, descriptive, cross-sectional design. A sample of 240 registered nurses were conveniently recruited to complete a self-administered online questionnaire. The data were coded and analyzed through SPSS version 24. Results: The participants had adequate knowledge on the prevention of DVT (75.64 ± 18.88), and the highest level was observed for knowledge about the prevention and prophylaxis of DVT (81.98 ± 45.73%). The practice level of nurses in preventing DVT was 71.92%, with a mean score of 18.7. Conclusions: There is a significant effect of nurses’ level of academic qualifications, working ward, and DVT prevention training on their knowledge and practice of DVT prevention.
Unexpected Products of Salicylidene-Aminoguanidine Reactions with Metal Ions—Synthesis and Structural Aspects
Authors: Radanović Mirjana M. , Vojinović-Ješić Ljiljana S. , Radulović Niko S. , Raičević Vidak N. , Leovac Vukadin M. , Rodić Marko V.
Published: December 2024

Due to the promising characteristics of aminoguanidine Schiff bases, ongoing research focuses on synthesizing and characterizing different compounds of this class to establish structure–property relationships. However, the pronounced alkalinity of the aminoguanidine residue makes isolating its Schiff bases in neutral form challenging. In the reaction of salicylidene-aminoguanidine ([HL]NO3) with a strong base (NaOH), the partially neutralized product of the formula [HL]NO3∙L·H2O was obtained in the form of single crystals. This compound could be considered a cocrystal in which protonated and neutral forms of the Schiff base coexist. Furthermore, the coordinating properties of [HL]NO3 towards zinc and organotin were investigated, and instead of the expected crystals of complex compounds, a novel polymorph of the ligand was obtained. Additionally, the reaction of [HL]NO3, NH4VO3 and salicylaldehyde was carried out to achieve the condensation of the free NH2-group in the aminoguanidinium fragment, targeting a vanadium(V) complex with tetradentate ligand. However, a purely organic compound containing three salicylaldehyde residues and two imine groups, i.e., C21H18N2O3, was isolated. All the obtained compounds were characterized by elemental and spectroscopic analysis, conductometry and SC-XRD analysis. The data were compared to those of similar structures, and the results provide further insight into the properties of these compounds and their future investigation for potential usage.
Antinociceptive Potential of Ximenia americana L. Bark Extract and Caffeic Acid: Insights into Pain Modulation Pathways
Authors: Pessoa Renata Torres , Santos da Silva Lucas Yure , Alcântara Isabel Sousa , Silva Tarcísio Mendes , Silva Eduardo dos Santos , da Costa Roger Henrique Sousa , da Silva Aparecida Barros , Ribeiro-Filho Jaime , Pereira Bezerra Martins Anita Oliveira Brito , Coutinho Henrique Douglas Melo , Sousa Jean Carlos Pereira , Chaves Andréa Rodrigues , Marreto Ricardo Neves , de Menezes Irwin Rose Alencar
Published: December 2024

Background/Objectives: This study evaluated the antinociceptive effect of the Ximenia americana L. bark extract (HEXA) and its primary component, caffeic acid (CA), through in vivo assays. Methods: The antinociceptive properties were assessed using abdominal writhing, hot plate, and Von Frey tests. Additionally, the study investigated the modulation of various pain signaling pathways using a pharmacological approach. Results: The results demonstrated that all doses of the HEXA significantly increased latency in the hot plate test, decreased the number of abdominal contortions, reduced hyperalgesia in the Von Frey test, and reduced both phases of the formalin test. Caffeic acid reduced licking time in the first phase of the formalin test at all doses, with the highest dose showing significant effects in the second phase. The HEXA potentially modulated α2-adrenergic (52.99%), nitric oxide (57.77%), glutamatergic (33.66%), vanilloid (39.84%), cyclic guanosine monophosphate (56.11%), and K+ATP channel-dependent pathways (38.70%). Conversely, CA influenced the opioid, glutamatergic (53.60%), and vanilloid (34.42%) pathways while inhibiting nitric oxide (52.99%) and cyclic guanosine monophosphate (38.98%). Conclusions: HEXA and CA exhibit significant antinociceptive effects due to their potential interference in multiple pain signaling pathways. While the molecular targets remain to be fully investigated, HEXA and CA demonstrate significant potential for the development of new analgesic drugs.
Exploring the Therapeutic Potential, Ethnomedicinal Values, and Phytochemistry of Helianthus tuberosus L.: A Review
Authors: Tapera Ruvimbo Faith , Siwe-Noundou Xavier , Shai Leshweni Jeremia , Mokhele Shoeshoe
Published: December 2024

Helianthus tuberosus L. (Jerusalem artichoke) tubers and aerial parts possess both nutritional and therapeutic properties. The Jerusalem artichoke has been utilized for various applications, including its use as a functional food source, a reservoir of bioactive compounds, and a raw material to produce biofuels. Moreover, the Jerusalem artichoke is a rich source of an indigestible polysaccharide called inulin, which serves as a prebiotic that improves gastrointestinal health. This plant has been used globally throughout history as a dietary supplement, for pain treatment, to reduce swelling, and to boost the immune system, as well as to treat skin wounds in folk medicine. It is an abundant source of bioactive compounds, such as phenolic acids, coumarins, and flavonoids, which are known to exert pharmacological activities, including antioxidant, antimicrobial, and anti-inflammatory properties. The literature on its potential as an antidiabetic, anticancer, anti-fungistatic, antiviral, and anti-obesity agent, among others, is scanty. This review aims to provide a comprehensive overview of Helianthus tuberosus L.’s traditional uses, nutritional properties, secondary bioactive compounds, and pharmacological properties to further explore its health benefits.
Fluoroquinolones and Biofilm: A Narrative Review
Authors: Geremia Nicholas , Giovagnorio Federico , Colpani Agnese , De Vito Andrea , Botan Alexandru , Stroffolini Giacomo , Toc Dan-Alexandru , Zerbato Verena , Principe Luigi , Madeddu Giordano , Luzzati Roberto , Parisi Saverio Giuseppe , Di Bella Stefano
Published: December 2024

Background: Biofilm-associated infections frequently span multiple body sites and represent a significant clinical challenge, often requiring a multidisciplinary approach involving surgery and antimicrobial therapy. These infections are commonly healthcare-associated and frequently related to internal or external medical devices. The formation of biofilms complicates treatment, as they create environments that are difficult for most antimicrobial agents to penetrate. Fluoroquinolones play a critical role in the eradication of biofilm-related infections. Numerous studies have investigated the synergistic potential of combining fluoroquinolones with other chemical agents to augment their efficacy while minimizing potential toxicity. Comparative research suggests that the antibiofilm activity of fluoroquinolones is superior to that of beta-lactams and glycopeptides. However, their activity remains less effective than that of minocycline and fosfomycin. Noteworthy combinations include fluoroquinolones with fosfomycin and aminoglycosides for enhanced activity against Gram-negative organisms and fluoroquinolones with minocycline and rifampin for more effective treatment of Gram-positive infections. Despite the limitations of fluoroquinolones due to the intrinsic characteristics of this antibiotic, they remain fundamental in this setting thanks to their bioavailability and synergisms with other drugs. Methods: A comprehensive literature search was conducted using online databases (PubMed/MEDLINE/Google Scholar) and books written by experts in microbiology and infectious diseases to identify relevant studies on fluoroquinolones and biofilm. Results: This review critically assesses the role of fluoroquinolones in managing biofilm-associated infections in various clinical settings while also exploring the potential benefits of combination therapy with these antibiotics. Conclusions: The literature predominantly consists of in vitro studies, with limited in vivo investigations. Although real world data are scarce, they are in accordance with fluoroquinolones’ effectiveness in managing early biofilm-associated infections. Also, future perspectives of newer treatment options to be placed alongside fluoroquinolones are discussed. This review underscores the role of fluoroquinolones in the setting of biofilm-associated infections, providing a comprehensive guide for physicians regarding the best use of this class of antibiotics while highlighting the existing critical issues.
Photonic Generation of Multiband and Multi-Format Chirped Microwave Waveforms Based on an Optoelectronic Oscillator
Authors: Du Jinfeng , Li Fangping , Tang Huiyun , Kong Zexuan , Li Ming , Li Wei
Published: December 2024

We propose and demonstrate a microwave photonic system to generate multiband and multi-format microwave waveforms based on an actively mode-locked optoelectronic oscillator (AML-OEO) using a dual-polarization dual-drive Mach–Zehnder modulator (DPol-DDMZM). In the proposed system, the upper DDMZM is injected by two baseband single-chirped signals, and the lower DDMZM is biased to realize single-sideband (SSB) modulation using a 90° hybrid coupler. The lower DDMZM is also used to construct an AML-OEO loop, which outputs microwave frequency comb signals. By setting the phase difference in the applied two single-chirped signals, multiband up-, down-, and dual-chirped microwave signals are successfully generated. Furthermore, the tunability of the system can be realized by adjusting the frequency and power of the injection signal in the AML-OEO loop, the passband of the electrical filter, and the chirp rate of the single-chirped signal. The proposed scheme is theoretically analyzed and experimentally verified.
Effects of Drought Stress at the Booting Stage on Leaf Physiological Characteristics and Yield of Rice
Authors: Yang Xiaolong , Wang Xiuxiu , Li Yang , Yang Lantian , Hu Long , Han Yuling , Wang Benfu
Published: December 2024

Drought stress is a major environmental constraint that limits rice (Oryza sativa L.) production worldwide. In this study, we investigated the effects of drought stress at the booting stage on rice leaf physiological characteristics and yield. The results showed that drought stress would lead to a significant decrease in chlorophyll content and photosynthesis in rice leaves, which would affect rice yield. Three different rice varieties were used in this study, namely Hanyou73 (HY73), Huanghuazhan (HHZ), and IRAT109. Under drought stress, the chlorophyll content of all cultivars decreased significantly: 11.1% and 32.2% decreases in chlorophyll a and chlorophyll b in HHZ cultivars, 14.1% and 28.5% decreases in IRAT109 cultivars, and 22.9% and 18.6% decreases in HY73 cultivars, respectively. In addition, drought stress also led to a significant decrease in leaf water potential, a significant increase in antioxidant enzyme activity, and an increase in malondialdehyde (MDA) content, suggesting that rice activated a defense mechanism to cope with drought-induced oxidative stress. This study also found that drought stress significantly reduced the net photosynthetic rate and stomatal conductance of rice, which, in turn, affected the yield of rice. Under drought stress, the yield of the HHZ cultivars decreased most significantly, reaching 30.2%, while the yields of IRAT109 and HY73 cultivars decreased by 13.0% and 18.2%, respectively. The analysis of yield composition showed that the number of grains per panicle, seed-setting rate, and 1000-grain weight were the key factors affecting yield formation. A correlation analysis showed that there was a significant positive correlation between yield and net photosynthetic rate, stomatal conductance, chla/chlb ratio, Rubisco activity, and Fv/Fm, but there was a negative correlation with MDA and non-photochemical quenching (NPQ). In summary, the effects of drought stress on rice yield are multifaceted, involving changes in multiple agronomic traits. The results highlight the importance of selecting and nurturing rice varieties with a high drought tolerance, which should have efficient antioxidant systems and high photosynthetic efficiency. Future research should focus on the genetic mechanisms of these physiological responses in order to develop molecular markers to assist in the breeding of drought-tolerant rice varieties.
Bioaccumulation of Cr by the Buddleja Species and Schinus molle L. Grown with and Without Compost in a Sandy Soil Contaminated by Leather Industrial Effluents
Authors: Huarsaya-Huillca Jamilet , Callo-Sánchez Sheyla , Aguilar-Ccuno Camila , Rodríguez-Salazar Oswaldo , Tupayachy-Quispe Danny , Romero-Mariscal Giuliana , Hachire-Patiño Zulema , Almirón Jonathan
Published: December 2024

This research aimed to assess the bioaccumulation capacity of the Buddleja species and Schinus molle L. using organic amendments to the phytoremediation of total chromium in the mid-zone of the Añashuayco Ravine, Uchumayo, Arequipa, impacted by tanneries from the Rio Seco Industrial Park. Additionally, it analyzed total chromium concentrations, soil physicochemical properties, and morphological changes in plants with and without organic matter. Samples of the Buddleja species and Schinus molle L. were distributed into groups with and without compost, along with control groups. They were monitored over 6 months, every 60 days, showing significant morphological variations. The results highlight an important finding: the remarkable bioaccumulation capacity of the species studied all exceeded 30%. The samples without compost showed a lower percentage of total chromium bioaccumulation in plants compared to the samples with the organic amendment. The Buddleja species demonstrated a 39.01% chromium bioaccumulation with compost compared to 37.99% without it. Likewise, Schinus molle L. achieved 33.99% chromium accumulation with compost and 31.84% without it. These findings emphasize the superior ability of these species to bioaccumulate heavy metals, highlighting that the Buddleja species has mayor bioaccumulation capacity and more remotion of total chromium in the soil.
Melting Behavior of Compression Molded Poly(ester amide) from 2,5-Furandicarboxylic Acid
Authors: Bianchi Enrico , Soccio Michelina , Gazzano Massimo , Papadopoulos Lazaros , Robert Tobias , Bikiaris Dimitrios N. , Lotti Nadia
Published: December 2024

PEA 46 is a biobased polymer with promising properties for sustainable packaging applications, which can be obtained via polymerization of a furan 2,5-dicarboxylic acid (2,5-FDCA) derivative and a diol monomer containing internal amide bonds (46 amido diol). In the literature, PEA 46 showed a complex series of thermal transitions during DSC scans. For this reason, in this initial exploratory study PEA 46 was subjected to compression molding and the melting behavior of film samples was investigated with parallel DSC and WAXS analyses. At room temperature, a mesomorph phase was the only one observed. Subjecting the samples to heating scans led to the formation of phase α, caused by a sequence of partially overlapping melting and recrystallization phenomena. An additional melting and recrystallization phenomenon resulted in the development of a phase β, which melted at approximately 173 °C, the temperature after which the material was completely amorphous and isotropic. Phase α could be enhanced via thermal annealing, whereas phase β could be enhanced via a melt crystallization treatment.
Preparation of Magnetic Hemicellulosic Composite Microspheres and Adsorption of Copper Ions
Authors: Sheraz Muhammad , Sun Xiao-Feng , Wang Yongke , Siddiqui Adeena , Chen Jiayi , Sun Le
Published: December 2024

In this study, the fabrication of magnetic hemicellulosic composite microspheres and the adsorption of copper ions are explored. The microspheres were prepared by the micro-emulsion technique, using Fe3O4 nanoparticles and hemicellulose extracted from wheat straw with the ionic liquid B[mim]Cl as a solvent. Fe3O4 nanoparticles, synthesized through coprecipitation, were evenly encapsulated within the hemicellulosic microspheres. The Fe3O4 nanoparticles measured 10–15 nm in size, while the microspheres had an average diameter of about 20 μm and displayed a saturation magnetization of 35.95 emu/g. The optimal conditions for copper adsorption by the microspheres were found to be a pH of 5.0, a temperature of 323 K, and an initial copper ion concentration of 80 mg/L, resulting in an adsorption capacity of 85.65 mg/g after 24 h. The adsorption kinetics followed a pseudo-second-order model, and the Langmuir isotherm suggested a monomolecular layer adsorption mechanism, with a theoretical maximum capacity of 149.25 mg/g. In summary, the magnetic hemicellulosic microspheres exhibited considerable adsorption potential and favorable recycling capabilities for copper ions.
In Vitro Hydroxyapatite Nucleation in Cationically Cured Epoxy Composites with Pulverized Date Seed
Authors: Atif Muhammad , Dilawaiz  , Akhtar Hafsah , Imran Muhammad , Ullah Muhammad Zafar , Andaleeb Hina , Hussain Muhammad Asif
Published: December 2024

Recently, driven by a growing focus on environmental sustainability and cost-effectiveness, researchers have shown a keen interest in creating useful materials from bio-wastes, particularly for their potential applications in the biomedical field. Current research has been conducted on the impact of date seed powder (DSP) on hydroxyapatite (HA) formation, specifically in relation to the promotion of bone health and regeneration. HA is an essential component of bone tissue and plays a crucial role in maintaining bone strength and structure. Date seed (DS) was used in two forms i.e., grains and powder, with unmodified and modified surface chemistries. Prepared composites were tested in vitro by soaking them in simulated body fluid (SBF). X-ray Diffraction (XRD) and Fourier Transform Infra-Red (FTIR) confirmed HA formation in all soaked samples. Thermogravimetric analysis (TGA) results indicated an improvement in thermal stability after soaking, suggesting a higher concentration of HA. Unsoaked samples were observed to have higher heat flow than soaked samples. The high gel content (GCs) over 90% and low hydrophilicity (less than 5%) of DSP-based composites were proven to be beneficial in HA nucleation. Antibacterial activity showed that the addition of DS filler yielded superior results compared to the pristine sample. Additionally, the modified samples demonstrated better antibacterial results than the unmodified ones.
Synthesis of Cyclic Oligomers of Polyether Ketone Ketone (PEKK) for Ring-Opening Polymerisation (ROP) Applications
Authors: Szmalko David , Evans Richard A. , Dilag Jessirie , Bateman Stuart
Published: December 2024

Entropy-Driven Ring-Opening Polymerisation represents an attractive mechanism to produce high-performance polymeric materials as it can be performed using neat, low-viscosity precursors and without the production of by-products or release of volatiles. Macrocyclic oligomers (MCOs) of polyether ketone ketone (PEKK) were synthesised and investigated as an in situ method of forming this high-performance thermoplastic. Cyclic oligomers were successfully synthesised by pseudo-high dilution methods, and the reaction conditions were optimised through careful addition of starting materials and carbonate base selection. These novel compounds were characterised, X-ray crystal structures were obtained, and the synthesis method was extended from the homopolymers to MCOs with the structural isomers predominantly used in industry. PEKK formed from MCOs were characterised by DSC, TGA and GPC and found to have similar glass transitions and molecular weight averages to those of a commercial PEKK polymer.
Finite Element Analysis and Improved Evaluation of Mechanical Response in Large Oil Storage Tanks Subjected to Non-Uniform Foundation Settlement
Authors: Jiao Yuanqi , Wang Yanbing , Li Jinzhou , Liu Xiaoben
Published: December 2024

This study developed a finite element model to address the issue of non-uniform settlement in large crude oil storage tanks. The model consisted of four key components: the tank foundation, bottom plate, wall plate, and large fillet welds. The Ramberg-Osgood model was used to describe the material’s nonlinearity. Key factors such as the radius-to-thickness ratio, height-to-diameter ratio, harmonic number, and amplitude were evaluated for their impact on the radial deformation of the tank’s top wall. Two numerical models were developed—one accounting for the coupling effect between the foundation and the tank bottom, and the other without it. The differences in radial deformation between these models were analyzed, revealing that deformation was minimally influenced by the radius-to-thickness ratio, but increased with higher height-to-diameter ratios and harmonic amplitudes. At low liquid levels, radial deformation increased with harmonic number, but at high levels, it decreased once the harmonic number exceeded four due to the decoupling of the tank bottom from the foundation. The model considering foundation coupling exhibited less radial deformation compared to the one neglecting it, particularly as the harmonic number and amplitude increased. An improved evaluation method identified a critical range of harmonic amplitudes for a 100,000 m3 tank, within which the coupling effect can be reasonably neglected, allowing deformation to be calculated using the simpler model.
The Effect of a Photoactivated Ruthenium Nitrocomplex [RuCl(NO2)(dppb)(4,4-2 Mebipy)] on the Viability of Eukaryotic and Prokaryotic Cells, Including Bacterial Biofilms
Authors: Andrade Alexandre L. , Pinheiro Aryane A. , Malveira Ellen A. , Azevedo Isa M. F. , Gondim Ana C. S. , Batista Alzir A. , Neto João H. A. , Castellano Eduardo E. , Holanda Alda K. M. , Vasconcelos Mayron A. , Teixeira Edson H.
Published: December 2024

Antimicrobial resistance (AMR) is a critical global public health problem. Many bacterial pathogens use biofilm formation as their main pathogenicity mechanism, a practical tactic for surviving in natural settings and colonized host tissues. Research using ruthenium(II) complexes has demonstrated antibacterial action linked to photodynamic therapy, an alternate method of microbial control. Thus, in this work, the photosensitive nitro complex [RuCl(NO2)(dppb)(4,4-Mebipy)] (I) was prepared and the X-ray structure was determined. Then, we investigated the antibacterial and antibiofilm activities, antibiotic-associated effects, and cytotoxicity. The results showed that complex I exhibited promising antimicrobial activity with MIC values ranging from 4 to 256 µg/mL and MBC from 4 to 32 µg/mL. The antimicrobial activity of this nitro complex was significantly enhanced with blue light irradiation, as confirmed by agarose gel electrophoresis of the pBR322 DNA, which must be related to the DNA cleavage promoted by the photorelease of NO. A synergistic effect against Staphylococcus aureus and Staphylococcus epidermidis strains was observed when combined with ampicillin, which exhibited FICI values from 0.186 to 0.311. Interestingly, complex I associated with tetracycline showed a synergistic effect only on Escherichia coli. Regarding biofilms, the irradiated complex I showed antibacterial activity against biofilm formation and mature biofilms. Furthermore, SEM and confocal analyses revealed changes in cell morphology and damage to the wall and plasma membrane. Complex I presented a percentage of hemolysis between 2 and 4%, and no cytotoxic effect was observed against murine dermal fibroblasts. In conclusion, the photoactivated ruthenium(II) complex showed antibacterial and antibiofilm activity against relevant bacteria.