bluuebunny/crossref_metadata_2025 huggingface dataset:
The role of endoscopy and findings in COVID-19 patients, an early North American Cohort
Authors:   North American Alliance for the Study of Digestive Manifestations of Covid-19, Kuftinec Gabriela , Elmunzer B. Joseph , Amin Sunil
Published: May 2021

Background and aims

Gastrointestinal manifestations in patients with COVID-19 are common but the role of endoscopy in this patient population remains unclear. We investigated the need for endoscopic procedures, their findings, and impact on patient care in a systematic and geographically diverse sample of patients hospitalized with COVID-19.

Methods

As part of the North American Alliance for the Study of Digestive Manifestations of COVID-19, we identified consecutive patients hospitalized with COVID-19 at 36 medical centers in the USA and Canada. We performed a secondary analysis of patients who underwent endoscopy, collecting information on endoscopic indications, findings, interventions, staffing, procedure location, anesthesia utilization, and adverse events.

Results

Data were collected on 1992 patients; 24 (1.2%) underwent 27 endoscopic procedures (18 upper endoscopies, 7 colonoscopies, 2 endoscopic retrograde cholangiopancreatographies). The most common indications were: gastrointestinal bleeding (13) and enteral access (6). The most common findings were erosive or inflammatory changes. Ten patients underwent an endoscopic intervention for hemostatic therapy (2), enteral access (6), or biliary obstruction (2). Half of cases employed anesthesiology support; no sedation-related adverse events were reported. One-third of cases were performed in the intensive care setting and one quarter in the endoscopy unit.

Conclusions

In this large, systematic, geographically diverse cohort of patients hospitalized with COVID-19 in North America, very few patients underwent endoscopy despite a high prevalence of gastrointestinal manifestations. Almost all endoscopic findings and interventions were thought related to critical illness rather than direct viral injury. This systematic assessment of endoscopic necessity and outcomes may help guide resource allocation in the event of ongoing and future surges.
Self-management interventions for adolescents living with HIV: a systematic review
Authors: Crowley Talitha , Rohwer Anke
Published: May 2021

Background

Self-management interventions aim to enable people living with chronic conditions to increase control over their condition in order to achieve optimal health and may be pertinent for young people with chronic illnesses such as HIV. Our aim was to evaluate the effectiveness of self-management interventions for improving health-related outcomes of adolescents living with HIV (ALHIV) and identify the components that are most effective, particularly in low-resource settings with a high HIV burden.

Methods

We considered randomised controlled trials (RCTs), cluster RCTs, non-randomised controlled trials (non-RCTs) and controlled before-after (CBA) studies. We did a comprehensive search up to 1 August 2019. Two authors independently screened titles, abstracts and full texts, extracted data and assessed the risk of bias. We synthesised results in a meta-analysis where studies were sufficiently homogenous. In case of substantial heterogeneity, we synthesised results narratively. We assessed the certainty of evidence using GRADE and presented our findings as summaries in tabulated form.

Results

We included 14 studies, comprising 12 RCTs and two non-RCTs. Most studies were conducted in the United States, one in Thailand and four in Africa. Interventions were diverse, addressing a variety of self-management domains and including a combination of individual, group, face-to-face, cell phone or information communication technology mediated approaches. Delivery agents varied from trained counsellors to healthcare workers and peers. Self-management interventions compared to usual care for ALHIV made little to no difference to most health-related outcomes, but the evidence is very uncertain. Self-management interventions may increase adherence and decrease HIV viral load, but the evidence is very uncertain. We could not identify any particular components of interventions that were more effective for improving certain outcomes.

Conclusion

Existing evidence on the effectiveness of self-management interventions for improving health-related outcomes of ALHIV is very uncertain. Self-management interventions for ALHIV should take into account the individual, social and health system contexts. Intervention components need to be aligned to the desired outcomes.

Systematic review registration

PROSPERO CRD42019126313.
Impact of high dose of baricitinib in severe COVID-19 pneumonia: a prospective cohort study in Bangladesh
Authors: Hasan Md. Jahidul , Rabbani Raihan , Anam Ahmad Mursel , Huq Shihan Mahmud Redwanul , Polash Mohammad Mufizul Islam , Nessa Shahzadi Sayeeda Tun , Bachar Sitesh C.
Published: May 2021

Purpose

Hyperinflammation in severe COVID-19 infection increases the risk of respiratory failure and one of the cogent reasons of mortality associated with COVID-19. Baricitinib, a janus kinases inhibitor, can potentially suppress inflammatory cascades in severe COVID-19 pneumonia.

Methods

The objective of this study was to compare the clinical outcomes of high dose of baricitinib with its usual dose in patients with severe COVID-19 pneumonia. This prospective cohort study was conducted on 238 adult patients with severe COVID-19 pneumonia. Eight milligram and 4 mg of baricitinib was given orally to 122 patients in the high dose (HD) group and 116 patients the usual dose (UD) group, respectively daily for 14 days, and clinical outcomes were compared among the groups.

Results

Blood oxygen saturation level was stabilized (≥94% on room air) earlier in the HD group compared to the UD group [5 (IQR: 4–5)/8 (IQR: 6–9),P < 0.05]. Patients in the HD group required intensive care unit (ICU) and intubation supports more in the UD group than that in patients of the HD group [17.2%/9%,P < 0.05; 11.2%/4.1%,P > 0.05;N = 116/122, respectively]. The 30-day mortality and 60-day rehospitalization rate were higher in the UD group than the HD group [6%/3.3%,P < 0.01; 11.9%/7.6%,P > 0.05;N = 116/122, respectively].

Conclusion

The daily high dose of baricitinib in severe COVID-19 results in early stabilization of the respiratory functions, declined requirements of critical care supports, reduced rehospitalization with mortality rate compared to its daily usual dose.
Prevalence of cystic echinococcosis in slaughtered livestock in Iran: a systematic review and meta-analysis
Authors: Vaisi-Raygani Aliakbar , Mohammadi Masoud , Jalali Rostam , Salari Nader , Hosseinian-Far Melika
Published: May 2021

Background

Hydatidosis is a zoonotic disease and has a great general and economic health importance in both developed and developing countries. Therefore, this systematic and meta-analytic study was conducted to determine the prevalence of cystic echinococcosis in slaughtered livestock in Iran.

Methods

The present study was conducted as a systematic review and meta-analysis. The SID & Magiran, MEDLINE (PubMed), ScienceDirect, Scopus, and Google Scholar databases were searched with a view to selecting relevant research works. As a result, 31 articles published from April 1970 to April 2020 were selected. The heterogeneity of the studies was assessed using the I2 index. Data analysis was conducted within the Comprehensive Meta-Analysis software (CMA) v.3.0 (Biostat, Englewood, NJ, USA) and Arc map (ArcGIS 10.3) software.

Results

The heterogeneity of the studies was evaluated using the I2 test which value was 99% showing a high heterogeneity in the studies. The results of publication bias in studies were evaluated by the Egger test, which were not statistically significant (P = 0.144). The overall prevalence of cystic echinococcosis in slaughtered livestock in Iran is 13.9% (95%CI: 10.7–17.7%). The results of the meta-regression analysis indicate the increasing trend of the hydatid cyst prevalence with the increase of sample size and publication year (P < 0.05).

Conclusion

According to the results of this study and the relatively high prevalence of cystic echinococcosis in slaughtered livestock in Iran, health policy makers should make effective decisions in this regard, and implement careful inspections and interventions by experts and health authorities.
Phase 3 multicenter randomized trial of PSMA PET/CT prior to definitive radiation therapy for unfavorable intermediate-risk or high-risk prostate cancer [PSMA dRT]: study protocol
Authors: Calais Jeremie , Zhu Shaojun , Hirmas Nader , Eiber Matthias , Hadaschik Boris , Stuschke Martin , Herrmann Ken , Czernin Johannes , Kishan Amar U. , Nickols Nicholas G. , Elashoff David , Fendler Wolfgang P.
Published: May 2021

Background

Definitive radiation therapy (dRT) is an effective initial treatment of intermediate-risk (IR) and high-risk (HR) prostate cancer (PCa). PSMA PET/CT is superior to standard of care imaging (CT, MRI, bone scan) for detecting regional and distant metastatic PCa. PSMA PET/CT thus has the potential to guide patient selection and the planning for dRT and improve patient outcomes.

Methods

This is a multicenter randomized phase 3 trial (NCT04457245). We will randomize 312 patients to proceed with standard dRT (control Arm, n = 150), or undergo a PSMA PET/CT scan at the study site (both 18F-DCFPyL and 68Ga-PSMA-11 can be used) prior to dRT planning (intervention arm, n = 162). dRT will be performed at the treating radiation oncologist facility. In the control arm, dRT will be performed as routinely planned. In the intervention arm, the treating radiation oncologist can incorporate PSMA PET/CT findings into the RT planning. Androgen deprivation therapy (ADT) is administered per discretion of the treating radiation oncologist and may be modified as a result of the PSMA PET/CT results. We assume that approximately 8% of subjects randomized to the PSMA PET arm will be found to have M1 disease and thus will be more appropriate candidates for long-term systemic or multimodal therapy, rather than curative intent dRT. PET M1 patients will thus not be included in the primary endpoint analysis. The primary endpoint is the success rate of patients with unfavorable IR and HR PCa after standard dRT versus PSMA PET-based dRT. Secondary Endpoints (whole cohort) include progression free survival (PFS), metastasis-free survival after initiation of RT, overall survival (OS), % of change in initial treatment intent and Safety.

Discussion

This is the first randomized phase 3 prospective trial designed to determine whether PSMA PET/CT molecular imaging can improve outcomes in patients with PCa who receive dRT. In this trial the incorporation of PSMA PET/CT may improve the success rate of curative intent radiotherapy in two ways: to optimize patient selection as a biomarker and to personalizes the radiotherapy plan.

Clinical trial registration

UCLA

IND#147591

○ Submission: 02.27.2020

○ Safe-to-proceed letter issued by FDA: 04.01.2020

UCLA IRB #20–000378

ClinicalTrials.gov Identifier NCT04457245. Date of Registry: 07.07.2020.

Essen

EudraCT 2020–003526-23
Impact of photoreceptor density in a 3D simulation of panretinal laser photocoagulation
Authors: Nishida Kentaro , Takahashi Shizuka , Sakaguchi Hirokazu , Sato Shigeru , Kanai Masanori , Shiraki Akihiko , Wakabayashi Taku , Hara Chikako , Fukushima Yoko , Sakimoto Susumu , Sayanagi Kaori , Kawasaki Ryo , Nishida Kohji
Published: May 2021

Background

During panretinal photocoagulation (PRP), the outer retina, especially the photoreceptors, are destroyed. During such procedures, the impact of the retinal photocoagulation, which is performed in the same photocoagulated area, may change if it is applied to different locations with different photoreceptor densities. Thus, we aimed to evaluate the influence of photoreceptor density on PRP.

Methods

We constructed a three-dimensional (3D) average distribution of photoreceptors with 3D computer-aided design (CAD) software using previously derived photoreceptor density data and calculated the number of photoreceptors destroyed by scatter PRP and full-scatter PRP (size 400-μm on the retina, spacing 1.0 spot) using a geometry-based simulation. To investigate the impact of photoreceptor density on PRP, we calculated the ratio of the number of photoreceptors destroyed to the total number of photoreceptors, termed the photoreceptor destruction index.

Results

In this 3D simulation, the total number of photoreceptors was 96,571,900. The total number of photoreceptors destroyed by scatter PRP and full-scatter PRP were 15,608,200 and 19,120,600, respectively, and the respective photoreceptor destruction indexes were 16.2 and 19.8%, respectively.

Conclusions

Scatter PRP is expected to have 4/5 of the number of photoreceptors destroyed by full-scatter PRP.
Climatic modification effects on the association between PM1 and lung cancer incidence in China
Authors: Guo Huagui , Li Xin , Li Weifeng , Wu Jiansheng , Wang Siying , Wei Jing
Published: May 2021

Background

Nationwide studies that examine climatic modification effects on the association between air pollution and health outcome are limited in developing countries. Moreover, few studies focus on PM1 pollution despite its greater health effect.

Objectives

This study aims to determine the modification effects of climatic factors on the associations between PM1 and the incidence rates of lung cancer for males and females in China.

Methods

We conducted a nationwide analysis in 345 Chinese counties (districts) from 2014 to 2015. Mean air temperature and relative humidity over the study period were used as the proxies of climatic conditions. In terms of the multivariable linear regression model, we examined climatic modification effects in the stratified and combined datasets according to the three-category and binary divisions of climatic factors. Moreover, we performed three sensitivity analyses to test the robustness of climatic modification effects.

Results

We found a stronger association between PM1 and the incidence rate of male lung cancer in counties with high levels of air temperature or relative humidity. If there is a 10 μg/m3 shift in PM1, then the change in male incidence rate relative to its mean was higher by 4.39% (95% CI: 2.19, 6.58%) and 8.37% (95% CI: 5.18, 11.56%) in the middle and high temperature groups than in the low temperature group, respectively. The findings of climatic modification effects were robust in the three sensitivity analyses. No significant modification effect was discovered for female incidence rate.

Conclusions

Male residents in high temperature or humidity counties suffer from a larger effect of PM1 on the incidence rate of lung cancer in China. Future research on air pollution-related health impact assessment should consider the differential air pollution effects across different climatic conditions.
Tributyltin perturbs femoral cortical architecture and polar moment of inertia in rat
Authors: Li Mingjun , Cheng Dong , Li Hui , Yao Wenhuan , Guo Dongmei , Wang Shu’e , Si Jiliang
Published: May 2021

Background

Tributyltin, a well-known endocrine disruptor, is widely used in agriculture and industry. Previous studies have shown that tributyltin could cause deleterious effects on bone health by impairing the adipo-osteogenic balance in bone marrow.

Methods

To investigate further the effects of tributyltin on bone, weaned male SD rats were treated with tributyltin (0.5, 5 or 50 μg·kg− 1) or corn oil by gavage once every 3 days for 60 days in this study. Then, we analyzed the effects of tributyltin on geometry, the polar moment of inertia, mineral content, relative abundances of mRNA from representative genes related to adipogenesis and osteogenesis, serum calcium ion and inorganic phosphate levels.

Results

Micro-computed tomography analysis revealed that treatment with 50 μg·kg− 1 tributyltin caused an obvious decrease in femoral cortical cross sectional area, marrow area, periosteal circumference and derived polar moment of inertia in rats. However, other test results showed that exposure to tributyltin resulted in no significant changes in the expression of genes detected, femoral cancellous architecture, ash content, as well as serum calcium ion and inorganic phosphate levels.

Conclusions

Exposure to a low dose of tributyltin from the prepubertal to adult stage produced adverse effects on skeletal architecture and strength.
Stochastic perturbation of the Lighthill–Whitham–Richards model via the method of stochastic characteristics
Authors: Müller Nora , Bock Wolfgang
Published: May 2021

In this paper we apply the method of stochastic characteristics to a Lighthill–Whitham–Richards model. The stochastic perturbation can be seen as errors in measurement of the traffic density. For concrete examples we solve the equation perturbed by a standard Brownian motion and the geometric Brownian motion without drift.
Recovery of bird communities following the construction of a large-scale hydroelectric dam
Authors: Nasruddin-Roshidi Affan , Mansor Mohammad Saiful , Ismail Nor Adibah , Ngadi Ehwan , Izzat-Husna Mohd , Husin Shahril Mod , Mohd-Taib Farah Shafawati , Illias Rahmah , Nor Shukor Md
Published: May 2021

Background

Habitat degradation is known to have a major impact on the composition of bird communities, yet how these communities recover following such disturbance is less understood. This study examined bird community changes from pre-logging to recovery over the course of habitat disturbance caused by the development of the Hulu Terengganu Hydroelectric Dam Project in Peninsular Malaysia, between 2007 and 2018. Bird surveys were conducted using point count observations and mist netting in the dam area from the pre-logging phase until the operation phase.

Results

It was observed that while bird species significantly declined after large-scale habitat clearance between the pre-logging and construction phases, they showed positive signs of recovery from the inundation to operation phases. These findings indicate that the bird community is still recovering, and the permanent loss of habitat required by most of the original species has not occurred, as evidenced by recolonisation. Bird species composition differed across phases due to varying habitat conditions. The turnover rate and immigration rate (recolonisation and newly recorded species) were higher between the construction and operation phases. It was also observed that insectivorous birds were more heavily affected than others by changes to the landscape, which highlights the versatility, survivability and tolerance of certain species to extreme disturbance and habitat modification.

Conclusion

Although the operation phase showed an incremental change in bird species richness due to recolonisation and newly recorded species, this value remains far from that of the pre-logging phase. We predict that bird species recovery will continue to increase for some time before reaching a plateau among the newly created islands, reservoir and catchment area of the dam.
Implementation of isoniazid preventive therapy in southern Lima, Peru: an analysis of health center characteristics
Authors: Zegarra-Chapoñan Roberto , Bonadonna Lily Victoria , Yuen Courtney M. , Martina-Chávez Martha Brigida , Zeladita-Huaman Jhon
Published: May 2021

Background

Tuberculosis (TB) prevention through the use of preventive treatment is a critical activity in the elimination of TB. In multiple settings, limited staffing has been identified as a barrier to managing preventive treatment for TB contacts. This study aims to determine how health center staffing, service type, and TB caseload affects implementation of isoniazid preventive therapy (IPT) for TB contacts in southern Lima.

Methods

We conducted an ecological study in 2019 in southern Lima, Peru. Through the review of medical records, we identified contacts of TB patients who initiated IPT during 2016–2018, and who were 0–19 years old, the age group eligible for IPT according to Peruvian guidelines. We assessed bivariate associations between health center characteristics (numbers of physicians and nurses, types of services available, annual TB caseload) and IPT initiation and completion using binomial logistic regression.

Results

Among 977 contacts, 69% took more than a week to start IPT and 41% did not complete IPT. For those who successfully completed IPT, 58% did not complete full medical follow-up. There was no significant difference in IPT completion or adherence based on whether health centers had more physicians and nurses, more comprehensive services, or higher TB caseloads. Among contacts, female sex was associated with delay in initiating IPT (P = 0.005), age 5–19 years old was associated with completion of IPT (P = 0.025) and age < 5 years old was associated with completion of clinical evaluations (P = 0.041).

Conclusions

There are significant gaps in IPT implementation in health centers of southern Lima, Peru, but insufficient staffing of health centers may not be responsible. Further research is needed to identify how IPT implementation can be improved, potentially through improving staff training or monitoring and supervision.

Graphic abstract
Illness perceptions predict distress in patients with chronic kidney disease
Authors: Muscat Priscilla , Weinman John , Farrugia Emanuel , Callus Roberta , Chilcot Joseph
Published: May 2021

Background

Patients diagnosed with chronic kidney disease (CKD) report increased distress associated with their clinical diagnosis. Distress in patients with predialysis CKD, has been linked to several adverse events; including increased risk of hospitalisation, early dialysis initiation and even death, suggesting that distress is a matter of great concern during routine care in predialysis CKD.

Aims

The present study aimed to assess the nature of illness perceptions and the level of distress in a CKD cohort diagnosed with different stages of kidney disease. It also aimed to explore the correlates of distress and to create a model for distress and its associated predictors making use of hierarchical regression analysis.

Methods

A sample of 200 patients diagnosed with Chronic Kidney Disease were recruited for this study from the nephrology outpatient clinics of Mater Dei Hospital, Malta. The participants were assessed for their; illness perceptions, treatment beliefs, level of depression and anxiety, coping style, as well as treatment adherence. Routine clinical information was also collected for participants, including a co-morbidity score.

Results

A percentage of 33.5% of the participants reported moderate distress, whilst 9.5% reported severe distress. Stronger illness identity, a perception of timeline as being increasingly chronic or cyclical in nature, greater consequences and higher emotional representations were associated with more advanced stages of CKD. In contrast, lower personal and treatment control and poorer illness coherence were associated with more advanced stages of CKD. Results from the hierarchical regression analysis showed that illness perceptions contributed significantly to distress over and above the clinical kidney factors. Being female, having low haemoglobin and specific illness perceptions including; perceptions of greater symptomatology, longer timeline, low personal control and strong emotional representations, as well as resorting to maladaptive coping, were all significantly associated with distress symptoms. Nevertheless, illness perceptions accounted for the greatest variance in distress thus indicating that the contribution of illness perceptions is greater than that made by the other known covariates.

Conclusion

Illness perceptions hold a principal role in explaining distress in CKD, relative to other traditional covariates. For this reason, illness perceptions should be addressed as a primary modifiable component in the development of distress in CKD.
The power of interest: minoritized women’s interest in engineering fosters persistence beliefs beyond belongingness and engineering identity
Authors: Verdín Dina
Published: May 2021

Backgrounds

This study examined how developing an engineering identity through the interplay between interest, recognition, and performance/competence beliefs and establishing a sense of belonging supported women’s persistence beliefs in engineering. Persistence belief in this study is captured through women’s certainty of graduating with an engineering degree. Students’ levels of motivation, affective states, and actions are based on what students believe to be true. Data were gathered from a survey administered to engineering students at nine institutions across the USA. Only female engineering students were used in the analysis. Students were further grouped into categories based on the representation of their race/ethnicity in engineering; 121 women were identified as minoritized in engineering, and 252 were identified as part of the majority group in engineering. Structural equation modeling was used to understand how the development of an engineering identity and modes of belonging (i.e., belonging in the major and in the classroom environment) supported women’s certainty to graduate with an engineering degree. All latent constructs were examined for measurement invariance; partial measurement invariance was achieved. Equality constraints on the structural paths of the model were not enforced to allow for differences across groups.

Results

Seeing oneself as an engineer (i.e., internal recognition) did not support minoritized women’s certainty to persist toward degree completion, whereas this internal recognition supported majority women’s persistence. Belonging in the major and belonging in the classroom environment did not support minoritized women’s certainty to persist. Establishing a sense of belonging in the classroom environment supported majority women’s certainty to persist. Minoritized women’s persistence toward degree completion was supported by their interest in engineering and their confidence in performing well in engineering coursework. However, interest in engineering was two times more influential toward minoritized women’s persistence than their performance competence beliefs.

Conclusion

These findings provide educators with a nuanced understanding of how identity development and modes of belonging differentially affect women’s persistence beliefs. These findings suggest that educators need to understand the powerful influence minoritized women’s interest in engineering has on their persistence beliefs and create mechanisms to continuously reinforce interest.
Inhibitory effect of low-molecular-weight peptides (0–3 kDa) from Spirulina platensis on H2O2-induced oxidative damage in L02 human liver cells
Authors: Ma Jun , Zeng Xiankun , Zhou Min , Cheng Le , Ren Difeng
Published: May 2021

Spirulina platensis protein hydrolysates were prepared by digesting protein extracts with papain, and the hydrolysates were separated into 30, 10, and 3 kDa weights using membrane ultrafiltration. The 0–3 kDa low-molecular-weight Spirulina peptides (LMWSPs) proved the highest chemical antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, hydroxyl radical (·OH) scavenging activities and total antioxidant capacity. Cellular antioxidant ability of LMWPs fractions against 2000 μg/mL H2O2 induced oxidative damage of L02 cells were investigated. The MTT assay results displayed that LMWSPs at different concentrations (0–1000 μg/mL) had proliferation effect on the L02 cells and that treatment of the L02 cells with the 1000 μg/mL LMWSPs (0–3 kDa) significantly prevented H2O2-induced oxidative damage compared with control cells. Moreover, the 2′,7′-dichlorofluorescein diacetate (DCFH-DA) fluorescent probe assay showed that the levels of ROS and NO were significantly lower in the experimental group that was treated with the peptides for 24 h than in the control group. Furthermore, using the corresponding kits, the treatment inhibited the reduction of SOD activity and the increase of MDA contents in the L02 cells. Therefore, LMWSPs (0–3 kDa) may have potential applications in antioxidant and liver health products.
Laparoscopic appendectomy for metastatic cervical cancer presenting as appendicitis
Authors: Fukai Shota , Lefor Alan Kawarai , Mizokami Ken
Published: May 2021

Background

Metastatic lesions to the appendix are rare. They usually present with acute appendicitis or remain asymptomatic and are diagnosed incidentally. Metastases to the appendix have been reported from a number of primary tumor sites including ovary, colon, gastric and lung. We report a laparoscopic appendectomy for a metachronous metastatic lesion to the appendix from the uterine cervix.

Case presentation

A 68-year-old woman, who underwent radical hysterectomy for cervical cancer 16 years previously, presented with nausea and gradually worsening right lower quadrant abdominal pain. Abdominal computed tomography scan showed an enlarged appendix and periappendiceal fat stranding. She was diagnosed with appendicitis and underwent laparoscopic appendectomy. Pathological findings showed adenocarcinoma in the submucosa and muscularis propria. Gastrointestinal endoscopy and positron emission tomography with computed tomography (PET–CT) did not show other lesions. Immunohistochemical analysis showed cytokeratin 7 (CK7) positive, cytokeratin 20 (CK20) negative, estrogen receptor (ER) 70–80% and progesterone receptor (PgR) 40–50%. The ER and PgR expression was similar to the cervical lesion 16 years previously, and the diagnosis was a metastatic lesion to the appendix from the uterine cervix.

Conclusions

Metastasis to the appendix from cancer of the uterine cervix is a rare lesion.
Antimicrobial, antioxidant, anti-inflammatory and cytotoxic study of extracts of Guibourtia tessmanii (harms) J. Léonard from Gabon
Authors: Obiang Cédric Sima , Misso Rick Léonid Ngoua Meye , Atome Guy Roger Ndong , Obame Rany Berty Mbeng , Ondo Joseph Privat , Engonga Louis Clément Obame , Emvo Edouard Nsi
Published: May 2021

Background

Gabonese flora abounds in a significant reserve of plants in medical matter. Thus, medicinal plants occupy a significant place in African pharmacopeia. Aim of this work was to evaluate the antimicrobial, antioxidant, anti-inflammatory and cytotoxic properties of extracts ofGuibourtia tessmanii(Harms) J. Léonard.

Methods

The test for sensitivity to microorganisms was performed by the diffusion method, while the MICs and MBCs were evaluated by the microdilution technique. Antioxidant tests were performed by scavenging the DPPH and ABTS radicals. Anti-inflammatory activity was determined by protein denaturing and membrane stabilization methods. The cytotoxicity was evaluated on the tadpoles of the green frog.

Results

The antibacterial activity shows that the Gt F2 fraction and the water-acetone extract produced the greatest inhibitions. The water, water-ethanol and water-acetone extracts exhibited bactericidal effects on the majority of bacteria. In the case of trapping of the DPPH radical, the IC50values varied from 6.92 ± 0.48 to 16.64 ± 0.20 μg/mL. For the decolouration of ABTS, oxidation was mainly inhibited by the water-acetetone, water-ethanol extracts and some fractions. The water and water-acetone extracts showed good inhibition of denaturation. The hemolysis test confirmed the good activities of the extracts. The lethal test showed that the LC50drops from 171.37 ± 9.25 to 58.25 ± 7.21 μg/mL after 24 and 96 h of exposure. In tadpoles exposed to 7.81 μg / mL of extracts, the first mortalities (12.5%) were observed on the second day of exposure. From the ninth day, the mortality rate increased (25%) until the 16th day.

Conclusion

Our results show thatGuibourtia tessmaniimay be a promising product for the isolations of molecules responsible for biological activities.
White-nose syndrome-related changes to Mid-Atlantic bat communities across an urban-to-rural gradient
Authors: Deeley Sabrina , Johnson Joshua B. , Ford W. Mark , Gates J. Edward
Published: May 2021

Background

White-nose Syndrome (WNS) has reduced the abundance of many bat species within the United States’ Mid-Atlantic region. To determine changes within the National Park Service National Capital Region (NCR) bat communities, we surveyed the area with mist netting and active acoustic sampling (2016–2018) and compared findings to pre-WNS (2003–2004) data.

Results

The results indicated the continued presence of the threatened Myotis septentrionalis (Northern Long-eared bat) and species of conservation concern, including Perimyotis subflavus (Tri-colored bat), Myotis leibii (Eastern Small-footed bat) and Myotis lucifugus (Little Brown bat). However, we documented a significant reduction in the abundance and distribution of M. lucifugus and P. subflavus, a decrease in the distribution of M. septentrionalis, and an increase in the abundance of Eptesicus fuscus (Big Brown bat).

Conclusions

Documented post-WNS M. septentrionalis recruitment suggests that portions of the NCR may be important bat conservation areas. Decreases in distribution and abundance of P. subflavus and M. lucifugus indicate probable extirpation from many previously occupied portions of the region.
Mechanisms of sterile inflammation after intravitreal injection of antiangiogenic drugs: a narrative review
Authors: Anderson William J. , da Cruz Natasha Ferreira Santos , Lima Luiz Henrique , Emerson Geoffrey G. , Rodrigues Eduardo Büchele , Melo Gustavo Barreto
Published: May 2021

Background

Intraocular inflammation is an uncommon but potentially vision-threatening adverse event related to anti-VEGF therapy. This is of increasing importance given both the volume of injections performed, as well as the increased prevalence of inflammation seen with newer anti-VEGF agents. Brolucizumab, the newest anti-VEGF agent, has been associated with an inflammatory retinal vasculitis and the underlying mechanism is unclear. Reviewing potential mechanisms and clinical differences of intraocular inflammation may assist clinicians and scientists in reducing the risk of these events in the future.

Observations

Two types of inflammation are seen with intravitreal injections, acute onset sterile inflammation and delayed onset inflammatory vasculitis. Acute onset inflammation can be subcategorized into subclinical anterior chamber inflammation and sterile uveitis/endophthalmitis. Subclinical anterior chamber inflammation can occur at rates as high as 19% after intravitreal anti-VEGF injection. Rates of sterile uveitis/endophthalmitis range from 0.05% to 4.4% depending on the anti-VEGF agent. Inflammatory vasculitis is only associated with brolucizumab and occurred in 3.3% of injections according to the post hoc review of the HAWK/HARRIER data. In addition, silicone oil from syringes can induce immunogenic protein aggregates. Agitation of the syringe, freeze thawing, shipping and improper storage prior to injection may increase the amount of silicone oil released from the syringe.

Conclusion

The main factors which play a role in intraocular inflammation after anti-VEGF injection can be divided into three causes: patient-specific, medication-specific and delivery-specific. The majority of clinically significant inflammation seen after intravitreal injection is an acute onset inflammatory response with most patients recovering baseline VA in 3–5 weeks. The presence of pain, hypopyon, severe anterior chamber reaction, hyperemia and significant vision loss may help distinguish infectious from non-infectious etiologies of post injection inflammation. Avoiding temperature fluctuation, mechanical shock, agitation during transport and handling of syringes/drugs, and the use of SO-free syringes may help minimize intraocular inflammation. While a definitive mechanism has not yet been established, current knowledge of the clinical presentation and vitreous histopathology of brolucizumab-retinal vasculitis favors an auto-immune type IV hypersensitivity reaction.
What do laboratory-forgetting paradigms tell us about use-inspired forgetting?
Authors: Scotti Paul S. , Maxcey Ashleigh M.
Published: May 2021

Directed forgetting is a laboratory task in which subjects are told to remember some information and forget other information. In directed forgetting tasks, participants are able to exert intentional control over which information they retain in memory and which information they forget. Forgetting in this task appears to be mediated by intentional control of memory states in which executive control mechanisms suppress unwanted information. Recognition-induced forgetting is another laboratory task in which subjects forget information. Recognizing a target memory induces the forgetting of related items stored in memory. Rather than occurring due to volitional control, recognition-induced forgetting is an incidental by-product of activating items in memory. Here we asked whether intentional directed forgetting or unintentional recognition-induced forgetting is a more robust forgetting effect. While there was a correlation between forgetting effects when the same subjects did both tasks, the magnitude of recognition-induced forgetting was larger than the magnitude of directed forgetting. These results point to practical differences in forgetting outcomes between two commonly used laboratory-forgetting paradigms.
Eocene to Oligocene nannofossils stratigraphy and environmental conditions in Izeh Province, Zagros Basin, East Tethys
Authors: Senemari Saeedeh , Jalili Farah
Published: May 2021

Data obtained from the calcareous nannofossils, distributed in the upper part of the Pabdeh Formation (Priabonian–Rupelian) and the lower part of the Asmari Formation (Chattian) in the Bid-Zard section, were used to investigate the Eocene to Oligocene palaeoenvironmental conditions in the southwest of Izeh, southwestern Iran (eastern Tethys). The upper part of the Pabdeh Formation was composed of shale, thin-bedded pelagic limestone and dolostone, which is disconformably overlain by the Asmari Formation. For the first time, 29 species of calcareous nannofossils belonging to 13 genera were identified in the studied section. The calcareous nannofossils in the upper part of the Pabdeh Formation indicate theIsthmolithus recurvusZone/Sphenolithus pseudoradiansZone (combined zone),Ericsonia subdistichaZone,Helicosphaera reticulataZone andSphenolithus praedistentusZone, from the Priabonian to the Rupelian. TheSphenolithus ciperoensisZone of the Chattian was identified in the lower part of the Asmari Formation. Calcareous nannofossil stratigraphy across the upper Eocene–Oligocene interval also reveals a disconformity at the Rupelian/Chattian transition due to a bio-event. Shallowing of the basin and environmental changes in this part of the Tethyan domain could have led to the lithostratigraphic and biostratigraphic changes. In fact, during the late Eocene to late Oligocene, marine phytoplankton was sensitive to climate changes such as decreasing temperature, as well as possibly to a nutrient increase and changes in basin depth.
Dual RNAseq highlights the kinetics of skin microbiome and fish host responsiveness to bacterial infection
Authors: Le Luyer J. , Schull Q. , Auffret P. , Lopez P. , Crusot M. , Belliard C. , Basset C. , Carradec Q. , Poulain J. , Planes S. , Saulnier D.
Published: May 2021

Background

Tenacibaculum maritimum is a fish pathogen known for causing serious damage to a broad range of wild and farmed marine fish populations worldwide. The recently sequenced genome of T. maritimum strain NCIMB 2154T provided unprecedented information on the possible molecular mechanisms involved in the virulence of this species. However, little is known about the dynamic of infection in vivo, and information is lacking on both the intrinsic host response (gene expression) and its associated microbiota. Here, we applied complementary omic approaches, including dual RNAseq and 16S rRNA gene metabarcoding sequencing using Nanopore and short-read Illumina technologies to unravel the host–pathogen interplay in an experimental infection system using the tropical fish Platax orbicularis as model.

Results

We showed that the infection of the host is characterised by an enhancement of functions associated with antibiotic and glucans catabolism functions but a reduction of sulfate assimilation process in T. maritimum. The fish host concurrently displays a large panel of immune effectors, notably involving innate response and triggering acute inflammatory response. In addition, our results suggest that fish activate an adaptive immune response visible through the stimulation of T-helper cells, Th17, with congruent reduction of Th2 and T-regulatory cells. Fish were, however, largely sensitive to infection, and less than 25% survived after 96 hpi. These surviving fish showed no evidence of stress (cortisol levels) or significant difference in microbiome diversity compared with controls at the same sampling time. The presence of T. maritimum in resistant fish skin and the total absence of any skin lesions suggest that these fish did not escape contact with the pathogen, but rather that some mechanisms prevented pathogens entry. In resistant individuals, we detected up-regulation of specific immune-related genes differentiating resistant individuals from controls at 96 hpi, which suggests a possible genomic basis of resistance, although no genetic variation in coding regions was found.

Conclusion

Here we focus in detail on the interplay between common fish pathogens and host immune response during experimental infection. We further highlight key actors of defence response, pathogenicity and possible genomic bases of fish resistance to T. maritimum.
Production of whey protein hydrolyzates and its incorporation into milk
Authors: John Jenny Ann , Ghosh Bikash C.
Published: May 2021

Whey proteins provide an excellent source of low-molecular-weight bioactive peptides with important functional properties and bioactivities like antihypertensive, opioid, and antimicrobial effects. Presence of peptide molecules with lower molecular weight has a great role in food for health promotion. In this investigation, the release of low-molecular-weight peptides from whey protein concentrate was attempted by using enzymatic digestion. The hydrolyzate was then incorporated into milk to obtain enriched milk (EM) with low-molecular-weight peptides. Based on sensory analysis of EM, electrophoretic and RP-HPLC studies, hydrolyzates of 10% protein (degree of hydrolysis 5%; enzyme/ substrate E/S, 1:50) were finally incorporated into milk at 20% (v/v) to develop an acceptable product enriched with low-molecular-weight peptides. EM had higher protein content, viscosity and emulsifying properties than control milk with 3% fat. It is recommended that EM should not be sterilized as it results in coagulation, but can be safely pasteurized and spray dried without any undesirable effects. Maximum ACE-inhibition activity was obtained in hydrolyzate, followed by EM. This study is expected to boost the opportunity for the dairy industry to venture further into the nutraceutical dairy market.

Graphical abstract
Toward regional hazard risk assessment: a method to geospatially inventory critical coastal infrastructure applied to the Caribbean
Authors: Becker Austin , Hallisey Noah , Bove Gerald
Published: May 2021

Hurricanes and sea level rise pose significant threats to infrastructure and critical services (e.g., air and sea travel, water treatment), and can hinder sustainable development of major economic sectors (e.g., tourism, agriculture, and international commerce). Planning for a disaster-resilient future requires high-resolution, standardized data. However, few standardized approaches exist for identifying, inventorying, and quantifying infrastructure lands at risk from natural hazards. This research presents a cost effective, standardized and replicable method to geospatially inventory critical coastal infrastructure land use and components, for use in risk assessments or other regional analyses. While traditional approaches to geospatial inventorying rely on remote sensing or techniques, such as object-based image analysis (OBIA) to estimate land use, the current approach utilizes widely available satellite imagery and a “standard operating procedure” that guides individual mappers through the process, ensuring replicability and confidence. As a pilot study to develop an approach that can be replicated for other regions, this manuscript focuses on the Caribbean. Small islands rely heavily on a small number of critical coastal infrastructure (airports, seaports, power plants, water and wastewater treatment facilities) and climate related hazards threaten sustainable development and economic growth. The Caribbean is a large and diverse area, and gaps exist between countries in the resources required for planning but much of the region lacks a comprehensive inventory of the land, infrastructure, and assets at risk. Identifying and prioritizing infrastructure at risk is the first step towards preserving the region’s economy and planning for a disaster resilient future. This manuscript uses high resolution satellite imagery to identify and geo-spatially classify critical infrastructure land area and assets, such as structures, equipment, and impervious surfaces. We identified 386 critical coastal infrastructure facilities across 28 Caribbean nations/territories, with over 19,000 ha of coastal land dedicated to critical infrastructure. The approach establishes a new standard for the creation of geospatial data to assess land use change, risk, and other research questions suitable for the regional scale, but with sufficient resolution such that individual facilities can utilize the data for local-scale analysis.
2D-wavelet based micro and macro texture analysis for asphalt pavement under snow or ice condition
Authors: Li Feng , Ablat Gulnigar , Zhou Siqi , Liu Yixin , Bi Yufeng , Weng Zihang , Du Yuchuan
Published: May 2021

In ice and snow weather, the surface texture characteristics of asphalt pavement change, which will significantly affect the skid resistance performance of asphalt pavement. In this study, five asphalt mixture types of AC-5, AC-13, AC-16, SMA-13, SMA-16 were prepared under three conditions of the original state, ice and snow. In this paper, a 2D-wavelet transform approach is proposed to characterize the micro and macro texture of pavement. The Normalized Energy (NE) is proposed to describe the pavement texture quantitatively. Compared with the mean texture depth (MTD), NE has the advantages of full coverage, full automation and wide analytical scale. The results show that snow increases the micro-scale texture because of its fluffiness, while the formation of the ice sheets on the surface reduces the micro-scale texture. The filling effect of snow and ice reduces the macro-scale texture of the pavement surface. In a follow-up study, the 2D-wavelet transform approach can be applied to improve the intelligent driving braking system, which can provide pavement texture information for the safe braking strategy of driverless vehicles.
Annexin A5 as a marker for hepatocellular carcinoma in cirrhotic hepatitis C virus patients
Authors: Serag Waleed Mohamed , Elsayed Basem Eysa
Published: May 2021

Background

The tumorigenesis and development in a variety of cancers is reportedly encouraged via Annexin A5. The levels of Annexin A5 were tested in patients with or without HCC who were affected by liver cirrhosis. The objective of our study was to detect Annexin A5 levels in such patients in order to assess their function as an HCC marker. The longitudinal study comprised 91 cirrhotic HCV patients with and without HCC, and 20 healthy volunteers in the control group approved by the National Hepatology and Tropical Medicine Research Institute (NHTMRI) between March 2017 and August 2018.The serum levels Annexin A5 were found in all groups with ELISA. ANOVA, Mann-Whitney, and χ2 tests had been applied.

Results

High scales of Annexin A5 (3.89 + 0.85) were recorded for cirrhosis with HCC, either than cirrhotic patients without HCC (3.06 ± 0.88) (P = 0.041), and either than the control group (0.54 ± 0.11) (P < 0.001).

Conclusion

In HCV cirrhotic patients with and without HCC, AnxA5 can be used as HCC marker.