bluuebunny/crossref_metadata_2025 huggingface dataset:
Autonomous Weapons Systems and Meaningful Human Control: Ethical and Legal Issues
Authors: Amoroso Daniele , Tamburrini Guglielmo
Published: August 2020

Purpose of Review

To provide readers with a compact account of ongoing academic and diplomatic debates about autonomy in weapons systems, that is, about the moral and legal acceptability of letting a robotic system to unleash destructive force in warfare and take attendant life-or-death decisions without any human intervention.

Recent Findings

A précis of current debates is provided, which focuses on the requirement that all weapons systems, including autonomous ones, should remain under meaningful human control (MHC) in order to be ethically acceptable and lawfully employed. Main approaches to MHC are described and briefly analyzed, distinguishing between uniform, differentiated, and prudential policies for human control on weapons systems.

The review highlights the crucial role played by the robotics research community to start ethical and legal debates about autonomy in weapons systems. A concise overview is provided of the main concerns emerging in those early debates: respect of the laws of war, responsibility ascription issues, violation of the human dignity of potential victims of autonomous weapons systems, and increased risks for global stability. It is pointed out that these various concerns have been jointly taken to support the idea that all weapons systems, including autonomous ones, should remain under meaningful human control (MHC). Main approaches to MHC are described and briefly analyzed. Finally, it is emphasized that the MHC idea looms large on shared control policies to adopt in other ethically and legally sensitive application domains for robotics and artificial intelligence.
Implementation of a Framework for an Abridged Review Using Good Reliance Practices: Optimising the Medicine Regulatory Review Process in South Africa
Authors: Keyter Andrea , Salek Sam , McAuslane Neil , Banoo Shabir , Azatyan Samvel , Walker Stuart
Published: August 2020

Background

This study sought to identify criteria and current practices for implementing an abridged review process and understanding barriers and enablers in utilizing reliance models and to offer recommendations for the implementation of an abridged review process in South Africa based on good reliance practices (GRelP).

Methods

A questionnaire was completed by six national regulatory authorities (NRAs) to determine criteria and current practices for implementing an abridged review process. In addition, two focus group discussions were conducted on the practical implementation of an abridged review process based on GRelP.

Results

Participating NRAs indicated that reliance would be placed on one reference agency. Applications submitted to NRAs for an abridged review had to be identical to those submitted to the reference agency. Unredacted reference agency assessment reports would be required to facilitate the abridged review process. A full technical dossier would also be required, but only parts would be assessed during the abridged review. Focus groups indicated that abridged review elements had been identified and should be considered in implementing GRelP.

Conclusions

NRAs strive to improve regulatory performance and accelerate approval times; however, many continue to face challenges due to resource constraints. Increasing workloads, advancing technologies, and limited expertise require NRAs to leverage regulatory convergence initiatives, collaborative registration procedures, and functional regional, continental and international networks to fulfil regulatory mandates. Recommendations for the implementation of an abridged review process and a framework for GRelP have been made with a view to optimise regulatory review processes in South Africa.
Evaluating the Success of ZaZiBoNa, the Southern African Development Community Collaborative Medicines Registration Initiative
Authors: Sithole Tariro , Mahlangu Gugu , Salek Sam , Walker Stuart
Published: August 2020

The Southern African Development Community (SADC) collaborative medicines registration initiative ZaZiBoNa is a successful regional work-sharing initiative on the African continent. This paper reviews the history of the ZaZiBoNa initiative, reflects on what has been realized in six years of operation and what still needs to be achieved. Statistics for the work done by the initiative are available in the literature, but there has not been a critical review of the process, including an analysis of factors contributing to the success of the initiative and conversely those negatively affecting performance. To do this, publicly available literature and statistics, meeting records, terms of reference and unpublished documents belonging to the initiative were reviewed. The successes of the ZaZiBoNa initiative can be attributed to leadership commitment, a clear vision and governance structure providing direction, and a clear, documented operating model, processes and objectives defined from the onset of the initiative. Closure of the gaps that were identified and implementation of the recommendations that were made in this paper will further strengthen the initiative. Furthermore, other regional harmonization or work-sharing initiatives on the African continent and beyond can draw lessons from this review of the ZaZiBoNa initiative for improved efficiency and effectiveness.
Impact of on-site compared to off-site testing for severe acute respiratory coronavirus virus 2 (SARS-CoV-2) on duration of isolation and resource utilization
Authors: Roberts Andrew T. , Wong Gabriella , Kotsanas Despina , Francis Michelle J. , Stuart Rhonda L. , Graham Maryza , Rogers Benjamin A.
Published: August 2020

Rapid detection and isolation of coronavirus disease 2019 (COVID-19) patients is the only means of reducing hospital transmission. We describe the impact of implementation of on-site severe acute respiratory coronavirus virus 2 (SARS-CoV-2) reverse-transcription polymerase chain reaction (RT-PCR) testing on reducing turnaround time, isolation duration, pathology test ordering, and antibiotic use in patients who do not have COVID-19.
Media Council of Tanzania and Others v. Attorney General of the United Republic of Tanzania (East Afr. Ct. J.)
Authors: Kamau Ndanga
Published: August 2020

On March 28, 2019, the First Instance Division of the East African Court of Justice (the EACJ or Court) issued a judgment in Media Council of Tanzania and Others v. Attorney General of the United Republic of Tanzania, Reference No. 2 of 2017. The Court held that several provisions of United Republic of Tanzania's Media Services Act 2016 (the Act) violated the freedom of expression at the heart of the fundamental and operational principles enshrined in Articles 6(d) and 7(2) of the Treaty Establishing the East African Community (the EAC Treaty).
On semidirectly closed non-aperiodic pseudovarieties of finite monoids
Authors: Kaďourek Jiří
Published: August 2020

It is shown that, for every prime number p, the complete lattice of all semidirectly closed pseudovarieties of finite monoids whose intersection with the pseudovariety G of all finite groups is equal to the pseudovariety Gp of all finite p-groups has the cardinality of the continuum. Furthermore, it is shown, in addition, that the complete lattice of all semidirectly closed pseudovarieties of finite monoids whose intersection with the pseudovariety G of all finite groups is equal to the pseudovariety Gsol of all finite solvable groups has also the cardinality of the continuum.
‘SLOW REVOLUTION’ IN SOUTHERN AFRICA: HOUSEHOLD BIOSOCIAL REPRODUCTION AND REGIONAL ENTANGLEMENTS IN THE HISTORY OF CATTLE-KEEPING AMONG NGUNI-SPEAKERS, NINTH TO THIRTEENTH CENTURY CE
Authors: Jimenez Raevin
Published: August 2020

In the eleventh to thirteenth century, Southern African Nguni-speakers made a counterintuitive choice to begin investing in large herds of cattle. Despite a long-standing knowledge of cattle, the earliest Nguni-speakers did not take to cattle-keeping as a way of life. Rather, the transition came as the result of changing social circumstances as households sought to manage the lifecycles of young men and reliably exploit their labor through gendered and generational expectations of decorum. Nguni-speakers grounded new concepts about cattle in older practices and norms regarding the social reproduction of young men. Agropastoralists situated cattle-keeping among the obligations young men faced after passing through initiation, giving cattle local salience. The transformation unfolded in gendered and generational household choices, but was shaped by the broad context of an increasingly interconnected Southern Africa.
The characterization of the cow-calf, stocker and feedlot cattle industry water footprint to assess the impact of livestock water use sustainability
Authors: Menendez H. M. , Tedeschi L. O.
Published: August 2020

Perception of freshwater use varies between nations and has led to concerns of how to evaluate water use for sustainable food production. The water footprint of beef cattle (WFB) is an important metric to determine current levels of freshwater use and to set sustainability goals. However, current WFBpublications provide broad WF values with inconsistent units preventing direct comparison of WFBmodels. The water footprint assessment (WFA) methodologies use static physio-enviro-managerial equations, rather than dynamic, which limits their ability to estimate cattle water use. This study aimed to advance current WFA methods for WFBestimation by formulating the WFA into a system dynamics methodology to adequately characterize the major phases of the beef cattle industry and provide a tool to identify high-leverage solutions for complex water use systems. Texas is one of the largest cattle producing areas in the United States, a significant water user. This geolocation is an ideal template for WFBestimation in other regions due to its diverse geography, management-cultures, climate and natural resources. The Texas Beef Water Footprint model comprised seven submodels (cattle population, growth, nutrition, forage, WFB, supply chain and regional water use; 1432 state variables). Calibration of our model replicated initial WFBvalues from an independent study by Chapagain and Hoekstra in 2003 (CH2003). This CH2003v.Texas production scenarios evaluated model parameters and assumptions and estimated a 41–66% WFBvariability. The current model provides an insightful tool to improve complex, unsustainable and inefficient water use systems.
First record of the rare deep-sea polychaete Rhamphobrachium (Rhamphobrachium) agassizii (Annelida: Onuphidae) in European waters
Authors: Arias Andrés , Paxton Hannelore
Published: August 2020

Rhamphobrachium (Rhamphobrachium) agassizii is reported from the Cantabrian Sea, Spain, from depths of 925–1207 m. This is its first record off the Iberian Peninsula and in European waters, representing its northernmost distribution in the North Atlantic Ocean to date. Previous reports of R. (R.) agassizii from the eastern and western North Atlantic demonstrate its apparent amphi-Atlantic distribution, which appears consistent with the distribution of the main Atlantic currents. It is a typical deep-water species with its deepest record at 2165 m from the Azores archipelago. The specimens were collected singly at two stations, attesting to the rarity of the species in contrast to its congener R. (Spinigerium) brevibrachiatum which was the most dominant polychaete species in a previous study.
Risk clusters of COVID-19 transmission in northeastern Brazil: prospective space–time modelling
Authors: Gomes D. S. , Andrade L. A. , Ribeiro C. J. N. , Peixoto M. V. S. , Lima S. V. M. A. , Duque A. M. , Cirilo T. M. , Góes M. A. O. , Lima A. G. C. F. , Santos M. B. , Araújo K. C. G. M. , Santos A. D.
Published: August 2020

This study aimed to analyse the trend and spatial–temporal clusters of risk of transmission of COVID-19 in northeastern Brazil. We conducted an ecological study using spatial and temporal trend analysis. All confirmed cases of COVID-19 in the Northeast region of Brazil were included, from 7 March to 22 May 2020. We used the segmented log-linear regression model to assess time trends, and the local empirical Bayesian estimator, the global and local Moran indexes for spatial analysis. The prospective space–time scan statistic was performed using the Poisson probability distribution model. There were 113 951 confirmed cases of COVID-19. The average incidence rate was 199.73 cases/100 000 inhabitants. We observed an increasing trend in the incidence rate in all states. Spatial autocorrelation was reported in metropolitan areas, and 178 municipalities were considered a priority, especially in the states of Ceará and Maranhão. We identified 11 spatiotemporal clusters of COVID-19 cases; the primary cluster included 70 municipalities from Ceará state. COVID-19 epidemic is increasing rapidly throughout the Northeast region of Brazil, with dispersion towards countryside. It was identified high risk clusters for COVID-19, especially in the coastal side.
Will there be any more classical scrapie cases in sheep in Great Britain? A modelling study to predict future cases
Authors: Arnold M.E. , Rajanayagam B.
Published: August 2020

The aim of this study was to apply a back-calculation model to Great Britain (GB) classical scrapie surveillance data, and use this model to estimate how many more cases might be expected, and over what time frame these cases might occur. A back-calculation model was applied to scrapie surveillance data between 2005 and 2019 to estimate the annual rate of decline of classical scrapie. This rate was then extrapolated to predict the number of future cases each year going forward. The model shows that there may be yet further cases of classical scrapie in GB. These will most likely occur in the fallen stock scheme, with approximately a 25% probability of at least 1 further scrapie positive, with a very low probability (~0.2%) of having up to three additional scrapie positives. This highlights the difficulty of completely eliminating all further cases, even in the presence of very effective control measures.
Characterisation of Bordetella bronchiseptica isolated from rabbits in Fujian, China
Authors: Wang J. , Sun S. , Chen Y. , Chen D. , Sang L. , Xie X.
Published: August 2020

Bordetella bronchiseptica is a potential zoonotic pathogen, which mainly causes respiratory diseases in humans and a variety of animal species. B. bronchiseptica is one of the important pathogens isolated from rabbits in Fujian Province. However, the knowledge of the epidemiology and characteristics of the B. bronchiseptica in rabbits in Fujian Province is largely unknown. In this study, 219 B. bronchiseptica isolates recovered from lung samples of dead rabbits with respiratory diseases in Fujian Province were characterised by multi-locus sequencing typing, screening virulence genes and testing antimicrobial susceptibility. The results showed that the 219 isolates were typed into 11 sequence types (STs) including five known STs (ST6, ST10, ST12, ST14 and ST33) and six new STs (ST88, ST89, ST90, ST91, ST92 and ST93) and the ST33 (30.14%, 66/219), ST14 (26.94%, 59/219) and ST12 (16.44%, 36/219) were the three most prevalent STs. Surprisingly, all the 219 isolates carried the five virulence genes (fhaB, prn, cyaA, dnt and bteA) in the polymerase chain reaction screening. Moreover, the isolates were resistant to cefixime, ceftizoxime, cefatriaxone and ampicillin at rates of 33.33%, 31.05%, 11.87% and 3.20%, respectively. This study showed the genetic diversity of B. bronchiseptica in rabbits in Fujian Province, and the colonisation of the human-associated ST12 strain in rabbits in Fujian Province. The results might be useful for monitoring the epidemic strains, developing preventive methods and preventing the transmission of epidemic strains from rabbits to humans.
Modeling the auxiliary phrase asymmetry in code-switched Spanish–English
Authors: Tsoukala Chara , Frank Stefan L. , Van Den Bosch Antal , Valdés Kroff Jorge , Broersma Mirjam
Published: August 2020

Spanish–English bilinguals rarely code-switch in the perfect structure between the Spanish auxiliary haber (“to have”) and the participle (e.g., “Ella ha voted”; “She has voted”). However, they are somewhat likely to switch in the progressive structure between the Spanish auxiliary estar (“to be”) and the participle (“Ella está voting”; “She is voting”). This phenomenon is known as the “auxiliary phrase asymmetry”. One hypothesis as to why this occurs is that estar has more semantic weight as it also functions as an independent verb, whereas haber is almost exclusively used as an auxiliary verb. To test this hypothesis, we employed a connectionist model that produces spontaneous code-switches. Through simulation experiments, we showed that i) the asymmetry emerges in the model and that ii) the asymmetry disappears when using haber also as a main verb, which adds semantic weight. Therefore, the lack of semantic weight of haber may indeed cause the asymmetry.
Perceptions of nutrition education classes offered in conjunction with a community-supported agriculture intervention among low-income families
Authors: Lu Isabel , Hanson Karla L , Jilcott Pitts Stephanie B , Kolodinsky Jane , Ammerman Alice S , Sitaker Marilyn , Wang Weiwei , Volpe Leah C , Belarmino Emily H , Garner Jennifer , Gonsalves Liana , Seguin Rebecca A
Published: August 2020

Objective:

To examine participants’ experiences with nutrition education classes that were implemented with and designed to complement a cost-offset community-supported agriculture (CSA) programme.

Design:

Qualitative analysis of data from twenty-eight focus groups with ninety-six participants enrolled in Farm Fresh Foods for Healthy Kids (F3HK). Transcribed data were coded and analysed by a priori and emergent themes.

Setting:

Rural and micropolitan communities in New York, North Carolina, Vermont and Washington (USA).

Participants:

Ninety-six F3HK participants.

Results:

Participants found recipes and class activities helpful and reported improvements in nutrition knowledge, food preservation skills and home cooking behaviours for themselves and their children; they also reported that classes promoted a sense of community. Some educators better incorporated CSA produce into lessons, which participants reported as beneficial. Other obligations and class logistics were barriers to attendance; participants recommended that lessons be offered multiple times weekly at different times of day. Other suggestions included lengthening class duration to encourage social engagement; emphasising recipes to incorporate that week’s CSA produce and pantry staples and offering additional strategies to incorporate children in classes.

Conclusion:

Complementing a cost-offset CSA with nutrition education may enhance programme benefits to low-income families by improving nutrition knowledge and cooking behaviours. However, future interventions will benefit from ongoing coordination between educators and local growing trajectories to maximise timely coverage of unfamiliar produce in lessons; synchronous scheduling of CSA pick-up and classes for participant convenience and creative strategies to engage children and/or provide childcare.
The role of dwelling type on food expenditure: a cross-sectional analysis of the 2015–2016 Australian Household Expenditure Survey
Authors: Oostenbach Laura H , Lamb Karen E , Dangerfield Fiona , Poelman Maartje P , Kremers Stef , Thornton Lukar
Published: August 2020

Objective:

To explore differences in proportion of food budget and total food expenditure by dwelling type.

Design:

A cross-sectional study using data from the Australian Bureau of Statistics 2015–2016 Household Expenditure Survey. Food expenditure was examined on multiple categories: fresh fruits, fresh vegetables, pre-prepared meals, meals in restaurants, hotels and clubs, and fast food and takeaway meals, using two-part models and zero-one inflated beta regression models. Dwelling types were categorised as separate house, semi-detached house, low-rise apartment and high-rise apartment.

Setting:

Australia, 2015–2016.

Participants:

Seven thousand three hundred and fifty-eight households from greater capital city areas.

Results:

Households living in high-rise apartments were estimated to allocate a greater proportion of their food budget to meals in restaurants, hotels and clubs, and to spend more (actual dollars) on that category, compared with other dwelling types. No substantial differences were estimated in the proportion of food budget allocated to the other food categories across dwelling types.

Conclusions:

The dwelling type households live in may play a role in their food budget. Households living in a high-rise apartment may potentially spend more on meals in restaurants, hotels and clubs than those living in other dwelling types. Given the growth in urban population and the changes in living arrangements, findings point to the critical need for a better understanding of the influence of dwelling types on food expenditure and call for research investigating the relationship between the two.
Assessment of DNA methylation pattern under drought stress using methylation-sensitive randomly amplified polymorphism analysis in rice
Authors: Sapna Harihar , Ashwini Narasimha , Ramesh Sampangiramareddy , Nataraja Karaba N.
Published: August 2020

DNA methylation is known to regulate gene expression when plants are exposed to abiotic stress such as drought. Therefore, insight into DNA methylation pattern would be useful for a better understanding of the expression profile of genes associated with drought adaptation. In the present study, we attempted to analyse the DNA methylation pattern at the whole-genome level and the expression of a few drought-responsive genes in rice under different regimes of soil water status, i.e. puddled, 100 and 60% field capacities (FC). The methylation-sensitive randomly amplified polymorphic DNA analysis was employed to identify DNA methylation pattern. We observed an increase in DNA methylation at 60% FC, and reduced methylation under 100% FC compared to puddled condition. The genes such as protein phosphatases (PP2C) and phenylalanine ammonia-lyase (PAL) having CpG islands in their promoter region had lower expression level under 100 and 60% FC compared to puddled conditions. Heat shock protein 70 (HSP70) and RNA helicase 25 (RH25), with no CpG islands in their promoter region, exhibited enhanced expression compared to puddled plants. In rice, increased DNA methylation seems to be an important mechanism associated with drought responses, which probably regulates the methylation-sensitive gene expression. The drought-induced changes in DNA methylation would contribute for epigenetic mechanism. The study provided evidence to argue that drought-induced increased methylation might be one of the major mechanisms associated with acclimation responses in field crops like rice.
DU BOIS AND MARX, DU BOIS AND MARXISM
Authors: Saman Michael J.
Published: August 2020

W. E. B. Du Bois’s engagement with the thought of Karl Marx forms an important aspect of his intellectual biography, yet its contours crystallize explicitly only late in his written work, and its development prior to the 1930s remains insufficiently understood. In order to bring to light the mix of criticisms, reservations, ideals, and inspirations that shape this reception, this article explores its trajectory as exhaustively as the available documentation permits, beginning from Du Bois’s early training in economics as a university student, continuing through his increasing attention to socialism in the early 1900s and his embrace of Soviet communism in the 1920s, and culminating in the 1930s in his teaching of Marx at Atlanta University and the overtly Marxian positions he adopts inBlack Reconstruction(1935).
Switchable design of a frequency reconfigurable broadband whip antenna in high frequency
Authors: Wang Hengfeng , Liu Chao
Published: August 2020

In this paper, the antenna reconfigurable technology is used to redesign a whip antenna in different sub-bands of high frequency (HF). According to the electrical characteristics of the antenna, on the one hand, two different radiation whip heights are designed to solve the problem of pattern up-warping in the high-frequency band; on the other hand, a common upper loading network and several different adjusting inductors and matching networks are designed for each sub-band to achieve high gain and efficiency when keeping good voltage standing wave ratio (VSWR) characteristics. Five sub-bands of the 10-m HF whip antenna are reconfigured through the actual selection of radiation height, adjusting inductance, and matching network by radio frequency (RF) switch. The antenna load and matching network are optimized by grasshopper optimization algorithm (GOA), and integrated into the antenna body by using the printed circuit technology. The scaled prototype of 1 m frequency reconfigurable antenna is manufactured and tested, which shows that the VSWR is all <3 with an average value of 2.14; the gain is all >−2.5 dB with an average value of 3.90 dB; the efficiency is all >18.2% with an average value of 71.59%, and the patterns all keep horizontal omnidirectional without the phenomenon of up-warping.
Functionality and efficacy of Franklin Robotics’ Tertill™robotic weeder
Authors: Sanchez Johnny , Gallandt Eric R.
Published: August 2020

Agricultural weeds remain an important production constraint, with labor shortages and a lack of new herbicide options in recent decades making the problem even more acute. Robotic weeding machines are a possible solution to these increasingly intractable weed problems. Franklin Robotics’ Tertill™is an autonomous weeding robot designed for home gardeners that relies on a minimalistic design to be cost-effective. The objectives of this study were to investigate the ability of the Tertill to control broadleaf and grass weeds, and based on early observations, experiments were conducted with and without its string-trimmer–like weeding implement. Tertill demonstrated high weed-control efficacy, supporting its utility as a tool for home gardeners. Weeds were best controlled by the combined effect of soil disturbance caused by the action of the robot’s wheels and the actuation of the string trimmer. Despite the regrowth potential of an annual grass due to its meristem location, Tertill maintained low densities of millet in an experimental arena. The simple and effective design of the Tertill may offer insights to inform future development of farm-scale weeding robots. Weed density, emergence periodicity, robot working rate, and robotic weeding mechanisms are important design criteria regardless of the technology used for plant detection.
The Role of Hiss, Chorus, and EMIC Waves in the Modeling of the Dynamics of the Multi‐MeV Radiation Belt Electrons
Authors: Drozdov A. Y. , Usanova M. E. , Hudson M. K. , Allison H. J. , Shprits Y. Y.
Published: August 2020

In this study, we performed a series of long‐term and individual storm simulations with and without hiss, chorus, and electromagnetic ion cyclotron (EMIC) waves. We compared simulation results incorporating different wave modes with Van Allen Probes flux observations to illustrate how hiss and chorus waves aid EMIC waves in depleting multi‐MeV electrons. We found that EMIC, hiss, and chorus waves are required to reproduce satellite measurements in our simulations. Our results indicate that hiss waves play a dominant role in scattering near‐equatorial mirroring electrons, and they assist EMIC waves, which scatter only small pitch angle electrons. The best agreement between the observations and the simulations (long‐term and 17 January 2013 storm) is achieved when hiss, chorus, and EMIC waves are included.
Factorial Sensitivity Analysis of Physical Schemes and Their Interactions in RegCM
Authors: Song Tangnyu , Huang Guohe , Wang Xiuquan , Zhou Xiong
Published: August 2020

It is well known that the choices of physical schemes in Regional Climate Models (RCMs) can cause considerable uncertainties in future climate projections. In this study, a factorial sensitivity analysis method has been proposed to screen out statistically significant schemes and interactions, which assists in selecting the optimized physical scheme combination from a long‐term perspective with affordable computational costs. The Regional Climate Model (RegCM) is used as an example to illustrate how the approach works. In detail, all schemes are fully tested through 120 experimental runs based on a factorial design; the contributions and statistical significance (P value) of individual schemes and their interactions to temperature, precipitation, wind speed, and wind direction are then quantified. The performance of the proposed approach is then demonstrated through a case study of Canada. The results indicate that there exist considerable spatial and temporal simulated variations associated with different scheme combinations. It is also suggested that individual physical schemes have dominant influences on simulated variations, but some effects explained by their interactions are statistically significant and thus cannot be neglected. In particular, the planetary boundary layer (PBL) scheme, moisture scheme, and land surface model are found to be the dominant factors affecting the uncertainties of temperature, precipitation, and wind speed in future climate projections over Canada, respectively. Furthermore, the potential relationships between the vegetation cover conditions and the sensitivity of physical schemes are explored. The proposed approach is an attempt to analyze the sensitivity influenced by not only individual physical schemes and also their multilevel interactions.
Roles of Double Low‐Level Jets in the Generation of Coexisting Inland and Coastal Heavy Rainfall Over South China During the Presummer Rainy Season
Authors: Liu Xi , Luo Yali , Huang Ling , Zhang Da‐Lin , Guan Zhaoyong
Published: August 2020

In this study, the key dynamic factors influencing the formation of six heavy‐rainfall events with coexisting inland and coastal rainfall over south China during May–June 2011–2017 are investigated using global ensemble forecasts from the European Centre for Medium‐Range Weather Forecasts. An ensemble sensitivity analysis and comparisons between the identified good and bad members are conducted. Results confirm the importance of the synoptic low‐level jet (SLLJ) and boundary layer jet (BLJ), but with different contributions, in determining the generation of the inland and coastal rainbelts, respectively. The inland heavy rainfall is closely related to both the SLLJ and BLJ, especially their meridional wind components. The more intense cold or quasi‐stationary front, low‐level vortex, or shear line accompanied by the stronger geostrophic southerly winds within both jets over inland and coastal south China is, the more rainfall over the inland region is favored. The ageostrophic southerly winds of the BLJ make limited contributions to the inland rainfall production but exert greater impacts on the generation of extreme (yet localized) coastal rainfall. The geostrophic meridional winds and associated low‐level front/vortex also play a nonnegligible but indispensable role in producing the coastal rainfall. These findings add to our knowledge of heavy rainfall in the East Asian monsoon region.
The saccharibacterium TM7x elicits differential responses across its host range
Authors: Utter Daniel R , He Xuesong , Cavanaugh Colleen M , McLean Jeffrey S , Bor Batbileg
Published: August 2020

Host range is a fundamental component of symbiotic interactions, yet it remains poorly characterized for the prevalent yet enigmatic subcategory of bacteria/bacteria symbioses. The recently characterized obligate bacterial epibiont Candidatus Nanosynbacter lyticus TM7x with its bacterial host Actinomyces odontolyticus XH001 offers an ideal system to study such a novel relationship. In this study, the host range of TM7x was investigated by coculturing TM7x with various related Actinomyces strains and characterizing their growth dynamics from initial infection through subsequent co-passages. Of the twenty-seven tested Actinomyces, thirteen strains, including XH001, could host TM7x, and further classified into “permissive” and “nonpermissive” based on their varying initial responses to TM7x. Ten permissive strains exhibited growth/crash/recovery phases following TM7x infection, with crash timing and extent dependent on initial TM7x dosage. Meanwhile, three nonpermissive strains hosted TM7x without a growth-crash phase despite high TM7x dosage. The physical association of TM7x with all hosts, including nonpermissive strains, was confirmed by microscopy. Comparative genomic analyses revealed distinguishing genomic features between permissive and nonpermissive hosts. Our results expand the concept of host range beyond a binary to a wider spectrum, and the varying susceptibility of Actinomyces strains to TM7x underscores how small genetic differences between hosts can underly divergent selective trajectories.
Polygenic risk for anxiety influences anxiety comorbidity and suicidal behavior in bipolar disorder
Authors: Lopes Fabiana L. , Zhu Kevin , Purves Kirstin L. , Song Christopher , Ahn Kwangmi , Hou Liping , Akula Nirmala , Kassem Layla , Bergen Sarah E. , Landen Mikael , Veras Andre B. , Nardi Antonio E. ,   Bipolar Genome Study Consortium (BiGS), Alliey-Rodriguez Ney , Badner Judith A. , Berrettini Wade , Byerley William , Coryell William , Craig David W. , Edenberg Howard J. , Foroud Tatiana , Gershon Elliot S. , Greenwood Tiffany A. , Guo Yiran , Keating Brendan J. , Koller Daniel L. , Lawson William B. , Liu Chunyu , Mahon Pamela B. , McInnis Melvin G. , Murray Sarah S. , Nurnberger John L. , Nwulia Evaristus A. , Panganiban Corrie B. , Rice John , Schork Nicholas J. , Smith Erin N. , Zhang Peng , Zöllner Sebastian , Goes Fernando S. , Kelsoe John R. , Nievergelt Caroline M. , Potash James B. , Shekhtman Tatyana , Schilling Paul D. , Zandi Peter P. , McMahon Francis J.
Published: August 2020

Bipolar disorder is often comorbid with anxiety, which is itself associated with poorer clinical outcomes, including suicide. A better etiologic understanding of this comorbidity could inform diagnosis and treatment. The present study aims to test whether comorbid anxiety in bipolar disorder reflects shared genetic risk factors. We also sought to assess the contribution of genetic risk for anxiety to suicide attempts in bipolar disorder. Polygenic risk scores (PRS) were calculated from published genome-wide association studies of samples of controls and cases with anxiety (n = 83,566) or bipolar disorder (n = 51,710), then scored in independent target samples (total n = 3369) of individuals with bipolar disorder who reported or denied lifetime anxiety disorders or suicidal attempts in research interviews. Participants were recruited from clinical and nonclinical settings and genotyped for common genetic variants. The results show that polygenic risk for anxiety was associated with comorbid anxiety disorders and suicide attempts in bipolar disorder, while polygenic risk for bipolar disorder was not associated with any of these variables. Our findings point out that comorbid anxiety disorders in bipolar disorder reflect a dual burden of bipolar and anxiety-related genes; the latter may also contribute to suicide attempts. Clinical care that recognizes and addresses this dual burden may help improve outcomes in people living with comorbid bipolar and anxiety disorders.
Large freshwater phages with the potential to augment aerobic methane oxidation
Authors: Chen Lin-Xing , Méheust Raphaël , Crits-Christoph Alexander , McMahon Katherine D. , Nelson Tara Colenbrander , Slater Gregory F. , Warren Lesley A. , Banfield Jillian F.
Published: August 2020

There is growing evidence that phages with unusually large genomes are common across various microbiomes, but little is known about their genetic inventories or potential ecosystem impacts. In the present study, we reconstructed large phage genomes from freshwater lakes known to contain bacteria that oxidize methane. Of manually curated genomes, 22 (18 are complete), ranging from 159 kilobase (kb) to 527 kb in length, were found to encode the pmoC gene, an enzymatically critical subunit of the particulate methane monooxygenase, the predominant methane oxidation catalyst in nature. The phage-associated PmoC sequences show high similarity to (>90%), and affiliate phylogenetically with, those of coexisting bacterial methanotrophs, including members of Methyloparacoccus, Methylocystis and Methylobacter spp. In addition, pmoC-phage abundance patterns correlate with those of the coexisting bacterial methanotrophs, supporting host–phage relationships. Future work is needed to determine whether phage-associated PmoC has similar functions to additional copies of PmoC encoded in bacterial genomes, thus contributing to growth on methane. Transcriptomics data from Lake Rotsee (Switzerland) showed that some phage-associated pmoC genes were highly expressed in situ and, of interest, that the most rapidly growing methanotroph was infected by three pmoC-phages. Thus, augmentation of bacterial methane oxidation by pmoC-phages during infection could modulate the efflux of this potent greenhouse gas into the environment.
Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria
Authors: Hörnlein Christine , Confurius-Guns Veronique , Grego Michele , Stal Lucas J. , Bolhuis Henk
Published: August 2020

Natural coastal microbial mat communities are multi-species assemblages that experience fluctuating environmental conditions and are shaped by resource competition as well as by cooperation. Laboratory studies rarely address the natural complexity of microbial communities but are usually limited to homogeneous mono-cultures of key species grown in liquid media. The mat-forming filamentous cyanobacteria Lyngbya aestuarii and Coleofasciculus chthonoplastes were cultured under different conditions to investigate the expression of circadian clock genes and genes that are under their control. The cyanobacteria were grown in liquid medium or on a solid substrate (glass beads) as mono- or as co-cultures under a light–dark regime and subsequently transferred to continuous light. TaqMan-probe based qPCR assays were used to quantify the expression of the circadian clock genes kaiA, kaiB, and kaiC, and of four genes that are under control of the circadian clock: psbA, nifH, ftsZ, and prx. Expression of kaiABC was influenced by co-culturing the cyanobacteria and whether grown in liquid media or on a solid substrate. Free-running (i.e. under continuous light) expression cycle of the circadian clock genes was observed in L. aestuarii but not in C. chthonoplastes. In the former organism, maximum expression of psbA and nifH occurred temporally separated and independent of the light regime, although the peak shifted in time when the culture was transferred to continuous illumination. Although functionally similar, both species of cyanobacteria displayed different 24-h transcriptional patterns in response to the experimental treatments, suggesting that their circadian clocks have adapted to different life strategies adopted by these mat-forming cyanobacteria.
On the role of microkinetic network structure in the interplay between oxygen evolution reaction and catalyst dissolution
Authors: Dam An Phuc , Papakonstantinou Georgios , Sundmacher Kai
Published: August 2020

Understanding the pathways of oxygen evolution reaction (OER) and the mechanisms of catalyst degradation is of essential importance for developing efficient and stable OER catalysts. Experimentally, a close coupling between OER and catalyst dissolution on metal oxides is reported. In this work, it is analysed how the microkinetic network structure of a generic electrocatalytic cycle, in which a common intermediate causes catalyst dissolution, governs the interplay between electrocatalytic activity and stability. Model discrimination is possible based on the analysis of incorporated microkinetic network structures and the comparison to experimental data. The derived concept is used to analyse the coupling of OER and catalyst dissolution on rutile and reactively sputtered Iridium oxides. For rutile Iridium oxide, the characteristic activity and stability behaviour can be well described by a mono-nuclear, adsorbate evolution mechanism and the chemical type of both competing dissolution and rate-determining OER-step. For the reactively sputtered Iridium oxide surface, experimentally observed characteristics can be captured by the assumption of an additional path via a low oxidation state intermediate, which explains the observed characteristic increase in OER over dissolution selectivity with potential by the competition between electrochemical re-oxidation and chemical dissolution.
Extrapyramidal plasticity predicts recovery after spinal cord injury
Authors: Huber E. , Patel R. , Hupp M. , Weiskopf N. , Chakravarty M. M. , Freund P.
Published: August 2020

Spinal cord injury (SCI) leads to wide-spread neurodegeneration across the neuroaxis. We explored trajectories of surface morphology, demyelination and iron concentration within the basal ganglia-thalamic circuit over 2 years post-SCI. This allowed us to explore the predictive value of neuroimaging biomarkers and determine their suitability as surrogate markers for interventional trials. Changes in markers of surface morphology, myelin and iron concentration of the basal ganglia and thalamus were estimated from 182 MRI datasets acquired in 17 SCI patients and 21 healthy controls at baseline (1-month post injury for patients), after 3, 6, 12, and 24 months. Using regression models, we investigated group difference in linear and non-linear trajectories of these markers. Baseline quantitative MRI parameters were used to predict 24-month clinical outcome. Surface area contracted in the motor (i.e. lower extremity) and pulvinar thalamus, and striatum; and expanded in the motor thalamus and striatum in patients compared to controls over 2-years. In parallel, myelin-sensitive markers decreased in the thalamus, striatum, and globus pallidus, while iron-sensitive markers decreased within the left caudate. Baseline surface area expansions within the striatum (i.e. motor caudate) predicted better lower extremity motor score at 2-years. Extensive extrapyramidal neurodegenerative and reorganizational changes across the basal ganglia-thalamic circuitry occur early after SCI and progress over time; their magnitude being predictive of functional recovery. These results demonstrate a potential role of extrapyramidal plasticity during functional recovery after SCI.
A preliminary study of mirror-induced self-directed behaviour on wildlife at the Royal Belum Rainforest Malaysia
Authors: Hamdan Azwan , Ab Latip Mohd Qayyum , Abu Hassim Hasliza , Mohd Noor Mohd Hezmee , Tengku Azizan Tengku Rinalfi Putra , Mohamed Mustapha Noordin , Ahmad Hafandi
Published: August 2020

Mirror-induced behaviour has been described as a cognitive ability of an animal to self-direct their image in front of the mirror. Most animals when exposed to a mirror responded with a social interactive behaviour such as aggressiveness, exploratory and repetitive behaviour. The objective of this study is to determine the mirror-induced self-directed behaviour on wildlife at the Royal Belum Rainforest, Malaysia. Wildlife species at the Royal Belum Rainforest were identified using a camera traps from pre-determined natural saltlick locations. Acrylic mirrors with steel frame were placed facing the two saltlicks (Sira Batu and Sira Tanah) and the camera traps with motion-detecting infrared sensor were placed at strategically hidden spot. The behavioural data of the animal response to the mirror were analysed using an ethogram procedure. Results showed that barking deer was the species showing the highest interaction in front of the mirror. Elephants displayed self-directed response through inspecting behaviour via usage of their trunk and legs while interacting to the mirror. Interestingly, the Malayan tapir showed startled behaviour during their interaction with the mirror. However, the absence of interactive behaviour of the Malayan tiger signalled a likelihood of a decreased social response behaviour. These results suggested that the ability to self-directed in front of the mirror is most likely related to the new approach to study the neural mechanism and its level of stimulus response in wildlife. In conclusion, research on mirror-induced self-directed behaviour in wildlife will have profound implications in understanding the cognitive ability of wildlife as an effort to enhance the management strategies and conservation.
Hydrocortisone decreases metacognitive efficiency independent of perceived stress
Authors: Reyes Gabriel , Vivanco-Carlevari Anastassia , Medina Franco , Manosalva Carolina , de Gardelle Vincent , Sackur Jérôme , Silva Jaime R.
Published: August 2020

It is well established that acute stress produces negative effects on high level cognitive functions. However, these effects could be due to the physiological components of the stress response (among which cortisol secretion is prominent), to its psychological concomitants (the thoughts generated by the stressor) or to any combination of those. Our study shows for the first time that the typical cortisol response to stress is sufficient to impair metacognition, that is the ability to monitor one’s own performance in a task. In a pharmacological protocol, we administered either 20 mg hydrocortisone or placebo to 46 male participants, and measured their subjective perception of stress, their performance in a perceptual task, and their metacognitive ability. We found that hydrocortisone selectively impaired metacognitive ability, without affecting task performance or creating a subjective state of stress. In other words, the single physiological response of stress produces a net effect on metacognition. These results inform our basic understanding of the physiological bases of metacognition. They are also relevant for applied or clinical research about situations involving stress, anxiety, depression, or simply cortisol use.
Panoramic ultrasound vs. MRI for the assessment of hamstrings cross-sectional area and volume in a large athletic cohort
Authors: Franchi Martino V. , Fitze Daniel P. , Hanimann Jonas , Sarto Fabio , Spörri Jörg
Published: August 2020

We investigated the validity of panoramic ultrasound (US) compared to magnetic resonance imaging (MRI) for the assessment of hamstrings cross-sectional area (CSA) and volume. Hamstrings CSA were acquired with US (by an expert operator) at four different sites of femur length (FL) in 85 youth competitive alpine skiers (14.8 ± 0.5 years), and successively compared to corresponding scans obtained by MRI, analyzed by a trained vs. a novice rater. The agreement between techniques was assessed by Bland–Altman analyses. Statistical analysis was carried out using Pearson’s product moment correlation coefficient (r). US-derived CSA showed a very good agreement compared to MRI-based ones. The best sites were 40% FL (0 = mid patellar point) for biceps femoris long head (r = 0.9), 50% for semitendinosus (r = 0.9), and 30% for semimembranosus (r = 0.86) and biceps femoris short head (BFsh, r = 0.8). US-based vs. MRI-based hamstrings volume showed an r of 0.96. Poorer r values were observed for the novice compared to the trained rater, with the biggest difference observed for BFsh at 50% (r = 0.001 vs. r = 0.50, respectively) and semimembranosus at 60% (r = 0.23 vs. r = 0.42, respectively). Panoramic US provides valid CSA values and volume estimations compared to MRI. To ensure optimal US-vs.-MRI agreement, raters should preferably possess previous experience in imaging-based analyses.