Data from Phase II Window Study of Olaparib Alone or with Cisplatin or Durvalumab in Operable Head and Neck Cancer
Authors: Moutafi Myrto , Koliou Georgia-Angeliki , Papaxoinis George , Economopoulou Panagiota , Kotsantis Ioannis , Gkotzamanidou Maria , Anastasiou Maria , Pectasides Dimitrios , Kyrodimos Efthymios , Delides Alexander , Giotakis Evangelos , Papadimitriou Nikolaos G. , Panayiotides Ioannis G. , Perisanidis Christos , Fernandez Aileen I. , Xirou Vasiliki , Poulios Christos , Gagari Eleni , Yaghoobi Vesal , Gavrielatou Niki , Shafi Saba , Aung Thazin Nwe , Kougioumtzopoulou Andromachi , Kouloulias Vassilis , Palialexis Konstantinos , Gkolfinopoulos Stavros , Strati Areti , Lianidou Evi , Fountzilas George , Rimm David L. , Foukas Periklis G. , Psyrri Amanda 
Published: August 2023

<div>Purpose:<p>We conducted a phase II randomized noncomparative window of opportunity (WOO) trial to evaluate the inhibition of cellular proliferation and the modulation of immune microenvironment after treatment with olaparib alone or in combination with cisplatin or durvalumab in patients with operable head and neck squamous cell carcinoma (HNSCC).</p>Experimental Design:<p>Forty-one patients with HNSCC were randomized to cisplatin plus olaparib (arm A), olaparib alone (arm B), no treatment (arm C) or durvalumab plus olaparib (arm D). The primary endpoint was to evaluate the percentage of patients in each arm that achieved a reduction of at least 25% in Ki67. Secondary endpoints included objective response rate (ORR), safety, and pathologic complete response (pCR) rate. Paired baseline and resection tumor biopsies and blood samples were evaluated for prespecified biomarkers.</p>Results:<p>A decrease in Ki67 of at least 25% was observed in 44.8% of treated patients, as measured by quantitative immunofluorescence. The ORR among treated patients was 12.1%. pCR was observed in 2 patients. Two serious adverse events occurred in 2 patients.</p><p>Programmed death ligand 1 (PD-L1) levels [combined positive score (CPS)] were significantly higher after treatment in arms A and D. Expression of <i>CD163</i> and colony-stimulating factor 1 receptor (<i>CSF1R</i>) genes, markers of M2 macrophages, increased significantly posttreatment whereas the expression of <i>CD80</i>, a marker of M1 macrophages, decreased.</p>Conclusion:<p>Preoperative olaparib with cisplatin or alone or with durvalumab was safe in the preoperative setting and led to decrease in Ki67 of at least 25% in 44.8% of treated patients. Olaparib-based treatment modulates the tumor microenvironment leading to upregulation of PD-L1 and induction of protumor features of macrophages.</p>Significance:<p>HNSCC is characterized by defective DNA repair pathways and immunosuppressive tumor microenvironment. PARP inhibitors, which promote DNA damage and “reset” the inflammatory tumor microenvironment, can establish an effective antitumor response. This phase II WOO trial in HNSCC demonstrated the immunomodulatory effects of PARP inhibitor–induced DNA damage. In this chemo-naïve population, PARP inhibitor–based treatment, reduced tumor cell proliferation and modulated tumor microenvironment. After olaparib upregulation of PD-L1 and macrophages, suggests that combinatorial treatment might be beneficial.</p>Synopsis:<p>Our WOO study demonstrates that preoperative olaparib results in a reduction in Ki67, upregulation of PD-L1 CPS, and induction of protumor features of macrophages in HNSCC.</p></div>