bluuebunny/crossref_metadata_2025 huggingface dataset:
Source-sink manipulation on yield contributing characters and yield of Sesame (Sesamum indicum L.)
Authors: Howlader MHK , Bain SK , Hasan MM , Khan ABMMM , Biswas S
Published: July 2018

Source-sink manipulation may improve light interception into the canopy and reduce the competition between vegetative and reproductive sinks during seed filling period, and may help in achieving higher yield. So, an effort was made to assess the effect of source-sink manipulation on yield contributing characters and yield of sesame. The experiment was laid out in two factors randomized complete block design consisted of two varieties of sesame viz.Binatil-3 and Local-Black and five levels of source-sink manipulation viz.control (M0), removal of lower empty leaves, lower empty branches and top of the inflorescence (M1), removal of top of the inflorescence (M2), removal of all branches and removal of lower empty leaves (M3) and lower empty branches (M4) with three replications. Source-sink manipulations were imposed during capsule development stage (50 days after emergence). Results revealed that the higher number of capsules plant-1 (16.17), seeds capsule-1 (53.27), maximum 1000-seed weight (2.72 g), higher seed capsule wall ratio (2.52), seed yield plant-1 (2.35 g), yield (938.96 kg ha-1) and harvest index (36.40%) were produced by the modern variety Binatil-3 than the traditional variety Local-black. Source-sink manipulation showed positive response to yield attributes compared with control. Maximum seeds capsule-1 (57.13), 1000-seed weight (2.92 g), higher seed capsule wall ratio (2.71), seed yield plant-1 (2.78 g) and yield (1110.96 kg ha-1) were found from removal of lower empty leaves, lower empty branches and top of the inflorescence (M1). Yield was increased by 71.77%, 46.88%, 8.52% and 22.45% due to M1, M2, M3 andM4 manipulation. Although all the source-sink manipulation treatments gave higher yield in their respective variety, the highest (1258.63 kg ha-1 and 125.04%) yield was obtained when lower leaves, lower empty branches and top of the inflorescence of the variety Binatil-3 were removed i.e. from V1M1 treatment. Therefore, Binatil-3 with removal of lower empty leaves, lower empty branches and top of the inflorescence manipulation was the best treatment in respect of yield and yield contributing characters of sesame.Progressive Agriculture 29 (1): 1-9, 2018
Understanding Heteroatom-Mediated Metal–Support Interactions in Functionalized Carbons: A Perspective Review
Authors: Campisi Sebastiano , Chan-Thaw Carine Edith , Villa Alberto
Published: July 2018

Carbon-based materials show unique chemicophysical properties, and they have been successfully used in many catalytic processes, including the production of chemicals and energy. The introduction of heteroatoms (N, B, P, S) alters the electronic properties, often increasing the reactivity of the surface of nanocarbons. The functional groups on the carbons have been reported to be effective for anchoring metal nanoparticles. Although the interaction between functional groups and metal has been studied by various characterization techniques, theoretical models, and catalytic results, the role and nature of heteroatoms is still an object of discussion. The aim of this review is to elucidate the metal–heteroatoms interaction, providing an overview of the main experimental and theoretical outcomes about heteroatom-mediated metal–support interactions. Selected studies showing the effect of heteroatom–metal interaction in the liquid-phase alcohol oxidation will be also presented.
Breast Cancer Stem Cells
Authors: Crabtree Judy S. , Miele Lucio
Published: July 2018

Breast cancer stem cells (BCSC) have been implicated in tumor initiation, progression, metastasis, recurrence, and resistance to therapy. The origins of BCSCs remain controversial due to tumor heterogeneity and the presence of such small side populations for study, but nonetheless, cell surface markers and their correlation with BCSC functionality continue to be identified. BCSCs are driven by persistent activation of developmental pathways, such as Notch, Wnt, Hippo, and Hedgehog and new treatment strategies that are aimed at these pathways are in preclinical and clinical development.
An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity
Authors: Zhang Sitong , Wu Zhuofu , Chen Guang , Wang Zhi
Published: July 2018

In this work, laccase from Trametes versicolor pretreated with copper ion solution was entrapped in copper alginate beads. The presence of laccase in copper alginate beads was verified by Fourier transform infrared (FTIR) spectroscopy. The alginate concentration used was optimized based on the specific activity and immobilization yield. After entrapment, laccase presents perfect pH stability and thermal stability with 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as the substrate. Moreover, laccase in copper alginate beads exhibits good reusability during continuous batch operation for removing 2,4-dichlorophenol. More importantly, owing to the coupled effect of copper ion activation and copper alginate entrapment, the entrapped laccase shows a 3.0-fold and a 2.4-fold increase in specific activity and 2,4-DCP degradation rate compared with that of free laccase, respectively.
Immobilization of Prunus amygdalus Hydroxynitrile Lyase on Celite
Authors: Bracco Paula , Torrelo Guzman , Noordam Sander , De Jong Glenn , Hanefeld Ulf
Published: July 2018

The hydroxynitrile lyase from Prunus amygdalus was immobilized on Celite R-633. The immobilized enzyme could successfully be utilized in buffer saturated MTBE and excellent conversions of benzaldehyde to R-mandelonitrile were observed. No leaching occurred. To achieve high enantioselectivities, the suppression of the undesired background reaction was essential. This could be achieved by high enzyme loadings and the tight packing of the immobilized enzymes. When the immobilized enzyme is loosely packed, both the enzyme catalysis and the background reaction accelerates and only a modest enantioselectivity is observed. The enzyme was recycled for up to ten times, with some loss of activity and also enantioselectivity after 5 cycles, independent of packing.
Appetite, Metabolism and Hormonal Regulation in Normal Ageing and Dementia
Authors: Nifli Artemissia-Phoebe
Published: July 2018

Feeding and nutrition follow the growth trajectory of the course of life. The profound physiological changes that human body experiences during ageing affect separate aspects of food intake, from tastant perception to satiety. Concurrent morbidities, such as neurodegeneration, as seen in dementia, and metabolic syndrome, may further shape nutritional behaviours, status and adequacy. In an effort to fill the gap between the exhausting basic research and the actual needs of professionals caring for the exponentially expanding ageing population, the current review addresses major factors relevant to appetite and eating disturbances. Does age alter the perception of food modalities? Is food generally still perceived as alluring and delicious with age? Is there an interplay between ageing, cognitive decline, and malnutrition? What tools can we adopt for proper and timely monitoring? Finally, what anatomical and pathophysiological evidence exists to support a hypothesis of central regulation of metabolic perturbations in normal and accelerated cognitive impairment, and how can we benefit from it in health practice?
An Improved Centralized Control Structure for Compensation of Voltage Distortions in Inverter-Based Microgrids
Authors: Afrasiabi Morteza , Rokrok Esmaeel
Published: July 2018

Recently, increased use of non-linear loads has intensified the harmonic distortion and voltage unbalance in distribution systems. Inverter Based Distributed Generators (IBDGs) can be employed as distributed compensators to improve the power quality, as well as to supply distribution systems. In this paper, an enhanced hierarchical control scheme for the compensation of voltage disturbance in an AC Micro Grid (MG) that includes of two control levels is proposed. The secondary control level is performed by a centralized controller. Data of voltage harmonics and voltage unbalance at the MG Sensitive Load Bus (SLB) is sent to the centralized controller by a measurement unit. A general case with a combined voltage harmonic and unbalance distortion is considered. The compensation coefficients for IBDG units are computed by the centralized controller, and reference commands are sent to the local controllers of the IBDG units that act as a primary level of control. In the secondary control level, harmonic analysis is performed for the MG in order to provide a guide for properly assigning the harmonics and unbalance compensation priorities to IBDGs at different locations in the distribution system. Some buses have more participation in exciting the MG resonance modes; therefore, larger harmonic compensation factors are considered for the IBDGs that are near to these buses. For other IBDGs, the voltage unbalance compensation factor is selected bigger. The control system of the IBDGs mainly includes a current controller, a virtual damping resistor loop, and a load compensation block. Effectiveness of the proposed control scheme is demonstrated through simulation studies.
Composite Estimators for Growth Derived from Repeated Plot Measurements of Positively-Asymmetric Interval Lengths
Authors: Roesch Francis A.
Published: July 2018

The statistical properties of candidate methods to adjust for the bias in growth estimates obtained from observations on increasing interval lengths are compared and contrasted against a standard set of estimands. This standard set of estimands is offered here as a solution to a varying set of user expectations that can arise from the jargon surrounding a particular data aggregation procedure developed within the USDA’s Forest Inventory and Analysis Program, specifically the term “average annual” growth. The definition of a standard set of estimands also allows estimators to be defined and the statistical properties of those estimators to be evaluated. The estimators are evaluated in a simulation for their effectiveness in the presence of a simple distribution of positively-asymmetric measurement intervals, such as what might arise subsequent to a reduction in budget being applied to a national forest inventory.
Effect of Fabric Integration on the Physical and Optical Performance of Electroluminescent Fibers for Lighted Textile Applications
Authors: Martin Alyssa , Fontecchio Adam
Published: July 2018

The advent of electroluminescent (EL) fibers, which emit light in response to an applied electric field, has opened the door for fabric-integrated light emission and displays in textiles. However, there have been few technical publications over the past few years about the performance of these light emitting fibers inside functional fabrics. Thus, there is limited information on the effect of integration on the physical and optical performance of such devices. In this work, alternating current powder-based EL (ACPEL) fibers were evaluated under a range of operating conditions both inside and outside of a knit matrix to understand how the EL fiber device performance changed inside a functional fabric. The device efficiency, adjustable brightness, and mechanical properties of these fibers are presented. The effects of fabric integration on the light-emitting fibers as well as the supporting knit fabric are discussed as they relate to the practical applications of this technology.
A Site Amplification Model for Crustal Earthquakes
Authors: Sandıkkaya M. Abdullah , Dinsever L. Doğan
Published: July 2018

A global dataset which is composed of more than 20,000 records is used to develop an empirical nonlinear soil amplification model for crustal earthquakes. The model also includes the deep soil effect. The soil nonlinearity is formulated in terms of input rock motion and soil stiffness. The input rock motion is defined by the pseudo-spectral acceleration at rock site condition (PSArock) which is also modified with between-event residual. Application of PSArock simplifies the usage of the site model by diminishing the need of using the period-dependent correlation coefficients in hazard studies. The soil stiffness is expressed by a Gompertz sigmoid function which restricts the nonlinear effects at both of the very soft soil sites and very stiff soil sites. In order to surpass the effect of low magnitude and long-distant recordings on soil nonlinearity, the nonlinear site coefficients are constrained by using a limited dataset. The coefficients of linear site scaling and deep soil effect are obtained with the full database. The period average of site-variability is found to be 0.43. The sigma decreases with decreasing the soil stiffness or increasing input rock motion. After employing residual analysis, the region-dependent correction coefficients for linear site scaling are also obtained.
ECG Electrode Placements for Magnetohydrodynamic Voltage Suppression
Authors: Gregory T. Stan , Oshinski John N. , Tse Zion Tsz Ho
Published: July 2018

This study aims to investigate a set of electrocardiogram (ECG) electrode lead locations to improve the quality of four-lead ECG signals acquired during magnetic resonance imaging (MRI). This was achieved by identifying electrode placements that minimized the amount of induced magnetohydrodynamic voltages (VMHD) in the ECG signals. Reducing VMHD can improve the accuracy of QRS complex detection in ECG as well as heartbeat synchronization between MRI and ECG during the acquisition of cardiac cine. A vector model based on thoracic geometry was developed to predict induced VMHD and to optimize four-lead ECG electrode placement for the purposes of improved MRI gating. Four human subjects were recruited for vector model establishment (Group 1), and five human subjects were recruited for validation of VMHD reduction in the proposed four-lead ECG (Group 2). The vector model was established using 12-lead ECG data recorded from Group 1 of four healthy subjects at 3 Tesla, and a gradient descent optimization routine was utilized to predict optimal four-lead ECG placement based on VMHD vector alignment. The optimized four-lead ECG was then validated in Group 2 of five healthy subjects by comparing the standard and proposed lead placements. A 43.41% reduction in VMHD was observed in ECGs using the proposed electrode placement, and the QRS complex was preserved. A VMHD-minimized electrode placement for four-lead ECG gating was presented and shown to reduce induced magnetohydrodynamic (MHD) signals, potentially allowing for improved cardiac MRI physiological monitoring.
Numerical Simulation of a Sandy Seabed Response to Water Surface Waves Propagating on Current
Authors: Tong Dagui , Liao Chencong , Chen Jinjian , Zhang Qi
Published: July 2018

An integrated numerical model is developed to study wave and current-induced seabed response and liquefaction in a flat seabed. The velocity-inlet wave-generating method is adopted in the present study and the finite difference method is employed to solve the Reynolds-averaged Navier-Stokes equations with k-ε turbulence closure. The model validation demonstrates the capacity of the present model. The parametrical study reveals that the increase of current velocity tends to elongate the wave trough and alleviate the corresponding suction force on the seabed, leading to a decrease in liquefaction depth, while the width of the liquefaction area is enlarged simultaneously. This goes against previous studies, which ignored fluid viscosity, turbulence and bed friction.
Housing Market Bubbles and Mortgage Contract Design: Implications for Mortgage Lenders and Households
Authors: Poitras Geoffrey , Zanotti Giovanna
Published: July 2018

This paper explores the implications of a housing market bubble for three critical elements of mortgage contract design: difference between term to maturity and amortization period; prepayment options; and, lender recourse in the event of default. Using an extension of classical immunization theory, this paper provides equilibrium conditions demonstrating the risk reduction benefits of shorter term to contract maturity at origination for lenders of long amortization mortgage contracts. In addition, the risks of underpricing prepayment and no recourse default options in the mortgage contract when compared with full recourse mortgage contracts having yield maintenance prepayment penalties are explored by contrasting the ability of US and Canadian mortgage funding systems to withstand a housing market bubble collapse that might occur.
Molecular Mechanisms Underlying Lubrication by Ionic Liquids: Activated Slip and Flow
Authors: Han Mengwei , Espinosa-Marzal Rosa M.
Published: July 2018

The present study provides molecular insight into the mechanisms underlying energy dissipation and lubrication of a smooth contact lubricated by an ionic liquid. We have performed normal and lateral force measurements with a surface forces apparatus and by colloidal probe atomic force microscopy on the following model systems: 1-ethyl-3-methyl imidazolium bis-(trifluoro-methylsulfonyl) imide, in dry state and in equilibrium with ambient (humid) air; the surface was either bare mica or functionalized with a polymer brush. The velocity-dependence of the friction force reveals two different regimes of lubrication, boundary-film lubrication, with distinct characteristics for each model system, and fluid-film lubrication above a transition velocity V∗. The underlying mechanisms of energy dissipation are evaluated with molecular models for stress-activated slip and flow, respectively. The stress-activated slip assumes that two boundary layers (composed of ions/water strongly adsorbed to the surface) slide past each other; the dynamics of interionic interactions at the slip plane and the strength of the interaction dictate the change in friction -decreasing, increasing or remaining constant- with velocity in the boundary-film lubrication regime. Above a transition velocity V∗, friction monotonically increases with velocity in the three model systems. Here, multiple layers of ions slide past each other (“flow”) under a shear stress and friction depends on a shear-activation volume that is significantly affected by confinement. The proposed friction model provides a molecular perspective of the lubrication of smooth contacts by ionic liquids and allows identifying the physical parameters that control friction.
Gut Microbiota and Mucosal Immunity in the Neonate
Authors: Dzidic Majda , Boix-Amorós Alba , Selma-Royo Marta , Mira Alex , Collado Maria Carmen
Published: July 2018

Gut microbiota colonization is a complex, dynamic, and step-wise process that is in constant development during the first years of life. This microbial settlement occurs in parallel with the maturation of the immune system, and alterations during this period, due to environmental and host factors, are considered to be potential determinants of health-outcomes later in life. Given that host–microbe interactions are mediated by the immune system response, it is important to understand the close relationship between immunity and the microbiota during birth, lactation, and early infancy. This work summarizes the evidence to date on early gut microbiota colonization, and how it influences the maturation of the infant immune system and health during the first 1000 days of life. This review will also address the influence of perinatal antibiotic intake and the importance of delivery mode and breastfeeding for an appropriate development of gut immunity.
A New Method to Recycle Stainless–Steel Duplex UNS S31803 Chips
Authors: Claudiney Mendonça , Adhimar Oliveira , Daniela Sachs , Patricia Capellato , Vander Ribeiro , Mateus Junqueira , Mirian Melo , Gilbert Silva
Published: July 2018

Due to the increased consumption of raw materials, energy, and the waste it generates, recycling has become very important and fundamental for the environment and the industrial sector. The production of duplex stainless–steel powders with the addition of vanadium carbide in the high energy mechanical milling process is a new method for recycling materials for the manufacture of components in the industrial sector. This study aims to reuse the chips from the duplex stainless–steel UNS S31803 by powder metallurgy with the addition of Vanadium carbide (VC). The mechanical milling was performed using a planetary ball mill for 50 h at a milling speed of 350 rpm and a ball-to-powder weight ratio of 20:1, and the addition of 3 wt % of VC. The material submitted to milling with an addition of carbide has a particle size of less than 140 μm. After milling, the sample went through a stress relief treatment performed at 1050 °C for 1 h and the isostatic compaction process loaded with 300 MPa. The sintered powders and material was characterized by scanning electron microscopy, X-ray diffraction, and micro-hardness tests. The milling process with an addition of 3% VC produced a particle size smaller than the initial chip size. The measurement of micrometric sizes obtained was between 26 and 132 μm. The sintered material had a measurement of porosity evaluated at 15%. The obtained density of the material was 84% compared to the initial density of the material as stainless–steel duplex UNS S31803. The value of the microhardness measurement was 232 HV. The material submitted for grinding presented the formation of a martensitic structure and after the thermal treatment, the presence of ferrite and austenite phases was observed. Thus, in conclusion, this study demonstrates the efficacy in the production of a metal-ceramic composite using a new method to recycle stainless–steel duplex UNS S31803 chips.
Anomeric O-Functionalization of Carbohydrates for Chemical Conjugation to Vaccine Constructs
Authors: Park Simon S. , Hsieh Hsiao-Wu , Gervay-Hague Jacquelyn
Published: July 2018

Carbohydrates mediate a wide range of biological interactions, and understanding these processes benefits the development of new therapeutics. Isolating sufficient quantities of glycoconjugates from biological samples remains a significant challenge. With advances in chemical and enzymatic carbohydrate synthesis, the availability of complex carbohydrates is increasing and developing methods for stereoselective conjugation these polar head groups to proteins and lipids is critically important for pharmaceutical applications. The aim of this review is to provide an overview of commonly employed strategies for installing a functionalized linker at the anomeric position as well as examples of further transformations that have successfully led to glycoconjugation to vaccine constructs for biological evaluation as carbohydrate-based therapeutics.
Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1
Authors: Ogawa Mitsutaka , Senoo Yuya , Ikeda Kazutaka , Takeuchi Hideyuki , Okajima Tetsuya
Published: July 2018

Extracellular O-GlcNAc is a novel class of modification catalyzed by epidermal growth factor-like (EGF)-domain specific O-GlcNAc transferase (EOGT). In mammals, EOGT is required for ligand-mediated Notch signaling for vascular development. Previous studies have revealed that O-GlcNAc in mammalian cultured cells is subject to subsequent glycosylation, which may impose additional layers of regulation. This study aimed to analyze the O-GlcNAc glycans of Drosophila EGF20 as model substrates and mouse Notch1 EGF repeats by mass-spectrometry. The analysis of Drosophila EGF20 expressed in HEK293T cells revealed that the majority of the proteins are modified with an elongated form of O-GlcNAc glycan comprising terminal galactose or sialic acid residues. In contrast, recombinant Notch1 EGF repeats isolated from HEK293T cells revealed structural divergence of O-GlcNAc glycans among the different EGF domains. Although the majority of Notch1 EGF2 and EGF20 domains contained the extended forms of the glycan, the O-GlcNAc in many other domains mostly existed as a monosaccharide irrespective of the exogenous EOGT expression. Our results raised a hypothesis that an array of O-GlcNAc monosaccharides may impact the structure and function of Notch receptors.
Oxidative Stability of Selected Edible Oils
Authors: Maszewska Magdalena , Florowska Anna , Dłużewska Elżbieta , Wroniak Małgorzata , Marciniak-Lukasiak Katarzyna , Żbikowska Anna
Published: July 2018

The aim of the study was to examine and compare oxidative stability of refined (peanut, corn, rice bran, grapeseed, and rapeseed) oils. The oils were subject a Schaal Oven Test (temperature 63 ± 1 °C) and a Rancimat test (temperature 120 °C) and their stability was compared at the 1st and 12th month of storage. Changes in the peroxide (PV) and anisidine (AnV) values in the thermostat test were the fastest in rapeseed oil and grapeseed oil. The best quality was preserved by peanut and corn oils both in the first and the twelfth month of storage. The induction times for the rice bran, corn, peanut, and rapeseed oils were similar from 4.77 h to 5.02 h in the first month and from 3.22 h to 3.77 h in the twelfth month. The shortest induction times were determined for grapeseed oil: 2.4 h and 1.6 h, respectively. A decrease of oxidative stability of about 30% was found in all the oils after 12 months of storage. The PV of 10, determined in the thermostat and Rancimat tests, were achieved at the latest in corn oil and the fastest in rice bran oil.
Preparation, Characterization, Pharmacokinetics and Biodistribution of Baicalin-Loaded Liposome on Cerebral Ischemia-Reperfusion after i.v. Administration in Rats
Authors: Li Nan , Feng Lingling , Tan Yujun , Xiang Yan , Zhang Ruoqi , Yang Ming
Published: July 2018

The dry root of Scutellaria baicalensis, has traditionally been applied in the treatment of cerebral ischemia in Chinese clinics. Baicalin (BA) is considered the key ingredient in it for the brain protection effects. The bioavailability of BA is very low because of its poor lipid and water solubility, which limits the therapeutic effects and clinical application. The aim of the present study was to develop a novel BA-loaded liposome (BA-LP) formulation to improve the drug lipophilicity and further to enhance the drug-concentration in the brain tissues. This study is also designed to investigate the pharmacokinetics of BA in the pathological conditions of stroke and evaluate the pharmacokinetic differences of BA caused by stroke after intravenous administration with BA and BA-LP. In this study, the novel BA-LP prepared in early stage were characterized by morphology, size, zeta potential, encapsulation rate and the in vitro release. The pharmacokinetics and biodistribution of BA and BA-LP were investigated by intravenous administration in rats with middle cerebral artery occlusion (MCAO) model and normal group respectively. BA-LP had a mean particle size of 160–190 nm, zeta potential of −5.7 mV, and encapsulation efficiency of 42 ± 1%. The BA-LP showed a sustained-release behavior, the in vitro drug-release kinetic model of BA-LP fit well with the biphasic dynamic model equation: Q = 1 − (60.12e0.56t − 59.08e0.0014t). Pharmacokinetic behavior in MCAO rats is not consistent with that of normal rats. The middle cerebral artery occlusion rats got higher Cmax and AUC0–t, which were about 1.5–2 times to normal rats both in BA and liposome groups. In addition, it got especially higher distribution in brain, while BA were not detected in brain tissues on normal rats. The Cmax and AUC0–t values were significantly greater with liposome than BA on both normal and MCAO rats. The tissue distribution behavior was significantly altered in the case of liposome administrated in comparison with BA, which the concentrations in the heart, liver, spleen, lungs and brain were all increased after administrated liposome, but decreased in kidneys. The TI values showed that the target of liposome was improved especially to heart, spleen and brain, and the brain’s target was higher in striatum and cerebellum. In conclusion, BA-LP might be a potential drug delivery system to improve the therapeutic efficacy of BA. In addition, these results also suggest that the pathological damages of ischemia-reperfusion have a significant impact on the pharmacokinetic traits of BA.
Immunogenicity and Induction of Functional Antibodies in Rabbits Immunized with a Trivalent Typhoid-Invasive Nontyphoidal Salmonella Glycoconjugate Formulation
Authors: Baliban Scott M. , Allen Jessica C. , Curtis Brittany , Amin Mohammed N. , Lees Andrew , Rao R. Nageswara , Naidu Gangadhara , Venkatesan Ramasamy , Rao D. Yogeswara , Mohan Vadrevu Krishna , Ella Krishna M. , Levine Myron M. , Simon Raphael
Published: July 2018

Typhoid fever due to Salmonella Typhi and invasive nontyphoidal Salmonella (iNTS) infections caused by serovars Enteritidis (SE) and Typhimurium (STm) are major pediatric health problems in sub-Saharan Africa. Typhoid has high complication rates, and iNTS infections have high case fatality rates; moreover, emerging antimicrobial resistance is diminishing treatment options. Vi capsule-based typhoid conjugate vaccine (Typbar-TCV™), licensed in India and pre-qualified by the World Health Organization, elicits durable immunity when administered to infants, but no iNTS vaccines are licensed or imminent. We have developed monovalent SE and STm glycoconjugate vaccines based on coupling lipopolysaccharide-derived core-O polysaccharide (COPS) to phase 1 flagellin protein (FliC) from the homologous serovar. Herein, we report the immunogenicity of multivalent formulations of iNTS COPS:FliC conjugates with Typbar-TCV™. Rabbits immunized with the trivalent typhoid-iNTS glycoconjugate vaccine generated high titers of serum IgG antibody to all three polysaccharide antigens for which anti-COPS IgG antibodies were directed primarily against serogroup-specific OPS epitopes. Responses to SE and STm FliC were lower relative to anti-COPS titers. Post-vaccination rabbit sera mediated bactericidal activity in-vitro, and protected mice after passive transfer against challenge with virulent SE or STm Malian blood isolates. These results support accelerated progression to clinical trials.
Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways
Authors: Cui Hong-Xin , Chen Ji-Hong , Li Jing-Wan , Cheng Fang-Rong , Yuan Ke
Published: July 2018

Myrica rubra (MR) is rich in anthocyanins, and it has good anti-cancer, anti-aging, antioxidant, and antiviral effects. The proportion of disability and death caused by ischemic stroke gradually increased, becoming a major disease that is harmful to human health. However, research on effects of anthocyanin from MR on cerebral ischemia-reperfusion (I/R) injury is rare. In this study, we prepared eight purified anthocyanin extracts (PAEs) from different types of MR, and examined the amounts of total anthocyanin (TA) and cyanidin-3-O-glucoside (C-3-G). After one week of PAE treatment, the cerebral infarction volume, disease damage, and contents of nitric oxide and malondialdehyde were reduced, while the level of superoxide dismutase was increased in I/R mice. Altogether, our results show that Boqi1 MR contained the most TA (22.07%) and C-3-G (21.28%), and that PAE isolated from Dongkui MR can protect the brain from I/R injury in mice, with the mechanism possibly related to the Toll-like receptor 4 (TLR4)/ nuclear factor-κB (NF-κB) and NOD-like receptor pyrin domain-containing 3 protein (NLRP3) pathways.
Effect of Beta Cyclodextrin on the Reduction of Cholesterol in Ewe’s Milk Manchego Cheese
Authors: Alonso Leocadio , Fox Patrick F. , Calvo María V. , Fontecha Javier
Published: July 2018

Beta-cyclodextrin (β-CD) is a cyclic oligosaccharide consisting of seven glucose units and is produced from starch using cyclodextrin glycotransferase enzymes to break the polysaccharide chain and forming a cyclic polysaccharide molecule. The use of β-CD in food research for reduction of cholesterol is increasing due to its affinity for non-polar molecules such as cholesterol. The aim of this study was to evaluate the feasibility of using β-CD in cholesterol removal from pasteurized ewe’s milk Manchego cheese and evaluate the effect on the main components of the milk, lipids, and flavor characteristics. Approximately 97.6% cholesterol reduction was observed in the cheese that was treated using β-CD. Physicochemical properties (fat, moisture and protein) were not changed by the β-CD treatment, except the soluble nitrogen and non-protein nitrogen that showed slight differences after the treatment. The amount of the different components of the lipid fraction (fatty acids, triglycerides and phospholipids) were similar in cheeses treated and not treated with β-CD. Flavor compound and short chain free fatty acids were not mostly significantly influenced by the effect of the β-CD. β-CD molecules are edible and nontoxic and as a result they can be used safely for cholesterol removal processing in cheese manufacturing. Therefore, the present study suggests that β-CD treatment is an effective process for cholesterol removal from Manchego cheese while preserving its properties.
Novel Pyrazole-Hydrazone Derivatives Containing an Isoxazole Moiety: Design, Synthesis, and Antiviral Activity
Authors: Yang Zaibo , Li Pei , Gan Xiuhai
Published: July 2018

In this study, a series of novel pyrazole-hydrazone derivatives containing an isoxazole moiety were synthesized. Antiviral bioassays indicated that some of the title compounds exhibited better in vivo antiviral activities against tobacco mosaic virus (TMV). In particular, compounds 6a, 6c and 6q exhibited the best curative activity, protection activity, and inactivation activity against TMV, respectively, which were superior to those of Ningnanmycin. This study demonstrated that this series of novel pyrazole-hydrazone derivatives containing an isoxazole amide moiety could effectively control TMV.
Induction of Different Sensitization Patterns of MRSA to Antibiotics Using Electroporation
Authors: Novickij Vitalij , Švedienė Jurgita , Paškevičius Algimantas , Markovskaja Svetlana , Lastauskienė Eglė , Zinkevičienė Auksė , Girkontaitė Irutė , Novickij Jurij
Published: July 2018

Treatment of bacteria-associated infections is complicated and antibiotic treatment alone is often inadequate to overcome biofilm infections. Physical methods allow overcoming this problem and propose solutions that are non-dependent on drug resistance. In this work, we investigated the feasibility of pulsed electric fields for sensitization of MRSA to common antibiotics. We analyzed the efficacy of inactivation of methicillin-resistant Staphylococcus aureus in 5–20 kV/cm electric field separately and in combination with gentamicin, doxycycline, ciprofloxacin, sulfamethoxazole, and vancomycin. Combined treatment allowed using up to 1000-fold smaller concentrations of antibiotics to induce the same inactivation of S. aureus.
Purification and Characterization of Lipase Produced by Leuconostoc mesenteroides Subsp. mesenteroides ATCC 8293 Using an Aqueous Two-Phase System (ATPS) Composed of Triton X-100 and Maltitol
Authors: Eko Sukohidayat Nurfadhilah Hidayah , Zarei Mohammad , Baharin Badlishah Sham , Manap Mohd Yazid
Published: July 2018

Purification of lipase produced by L. mesenteroides subsp. mesenteroides ATCC 8293 was conducted for the first time using a novel aqueous two-phase system (ATPS) composed of Triton X-100 and maltitol. The partitioning of lipase was optimized according to several parameters including pH, temperature, and crude load. Results showed that lipase preferentially migrated to the Triton X-100 rich phase and optimum lipase partitioning was achieved in ATPS at TLL of 46.4% and crude load of 20% at 30 °C and pH 8, resulting in high lipase purification factor of 17.28 and yield of 94.7%. The purified lipase showed a prominent band on SDS-PAGE with an estimated molecular weight of 50 kDa. The lipase was stable at the temperature range of 30–60 °C and pH range of 6–11, however, it revealed its optimum activity at the temperature of 37 °C and pH 8. Moreover, lipase exhibited enhanced activity in the presence of non-ionic surfactants with increased activity up to 40%. Furthermore, results exhibited that metals ions such as Na+, Mg2+, K+ and Ca2+ stimulated lipase activity. This study demonstrated that this novel system could be potentially used as an alternative to traditional ATPS for the purification and recovery of enzymes since the purified lipase still possesses good process characteristics after undergoing the purification process.
The Polysaccharides from Codonopsis pilosula Modulates the Immunity and Intestinal Microbiota of Cyclophosphamide-Treated Immunosuppressed Mice
Authors: Fu Yu-Ping , Feng Bin , Zhu Zhong-Kai , Feng Xin , Chen Shu-Fan , Li Li-Xia , Yin Zhong-Qiong , Huang Chao , Chen Xing-Fu , Zhang Bing-Zhao , Jia Ren-Yong , Song Xu , Lv Cheng , Yue Gui-Zhou , Ye Gang , Liang Xiao-Xia , He Chang-Liang , Yin Li-Zi , Zou Yuan-Feng
Published: July 2018

Based on previous studies about microflora regulation and immunity enhancement activities of polysaccharides from Codonopsis pilosula Nannf. var. modesta (Nannf.) L. T. Shen (CPP), there is little study on intestinal mucosal immunity, which is a possible medium for contacting microflora and immunity. In the present study, the BALB/c mice were divided into five groups (eight mice in each group), including a normal group (Con), a model control group (Model), and model groups that were administered CPP (50, 100, 200 mg/kg/d) orally each day for seven days after intraperitoneal injection of 60 mg/kg BW/d cyclophosphamide (CP) for three days. CPP recovered the spleen index and restored the levels of IFN-γ, IL-2, IL-10, as well as serum IgG. In addition, it elevated ileum secretory immunoglobulin A (sIgA), the number of Lactobacillus and acetic acid content in cecum. These results indicated that CPP plays an important role in the protection against immunosuppression, especially mucosa immune damage, and the inhibition of pathogenic bacteria colonization, which could be considered a potential natural source of immunoregulator.
Regional Variation in Pharmacist Perception of the Financial Impact of Medicare Part D
Authors: Khan Shamima , Spooner Joshua J. , Spotts Harlan E.
Published: July 2018

The objective of this study was to perform a nationwide investigation of the financial performance of community pharmacies in the United States since the inception of Medicare Part D. A nationwide, cross-sectional survey of pharmacists was conducted in 2013. The 43-item online survey collected information about demographics, financial implications of Part D on community pharmacy and patients, provision of Medication Therapy Management (MTM) services and opinions about Medicare Part D 2010 updates. The adjusted response rate was 22.3% (419/1885). A majority of respondents (75.6%) reported a stable or increased prescription volume since 2006 but only 40.4% indicated that the financial performance of their pharmacy as either excellent or good during the same period. Owners and part-owners of rural independent pharmacies were more likely to report a below average or poor financial performance (75.0%). The provision of MTM services was not related to the financial performance of the pharmacy. Nearly half (44.7%) of pharmacy owners or part-owners indicated that they were considering selling their pharmacy, with most (94.1%) reporting that their decision to sell was due to the Part D financial pressures. However, the decision to sell was not related to the change in financial performance since 2006 or the volume of prescriptions dispensed.
Effects of Waste Management Customer Online Value Co-Creation on Sanitation Attitude and Advocacy: A Customer-Enterprise Dyadic Perspective
Authors: Frempong Joseph , Chai Junwu , Ampaw Enock Mintah
Published: July 2018

The study aims at establishing the benefits of actively utilizing the intangible resources of solid waste management customers in designing and implementing solid waste collection services, using the social media platforms. While Ghana generates high volumes of solid waste on a daily basis, less than half of it is effectively collected and disposed of. This calls for the adoption of innovative strategies to better connect and serve customers. Adopting a marketing approach to solid waste management has not been given much needed attention in Ghana and Africa, and this research sought to contribute in that direction. There is high usage of mobile telephony services in Ghana which a waste firm can explore to change negative attitude to waste disposal by the populace. Online co-creation is seen as a modern marketing approach leading to behavioral change in consumers. In this regard, the study looked at customer online co-creation in the solid waste collection sector in Ghana. The study adopted the survey strategy using structured questionnaire as the measure instrument, and data analyzed using both the structural equation model (SEM) and hierarchical multiple regression. The key findings are that customer intangible resources (online experience and skills) can be tapped by waste firms to co-create services that would generate positive attitude towards sanitation issues and the willingness to advocate the services and programs of the firm. Similarly, waste firms must invest in well-functioning and information rich digital platforms, and to devise innovative strategies to direct traffic to these platforms for effective customer participation.
The Development of an Infrastructure Quality Index for Nigerian Metropolitan Areas Using Multivariate Geo-Statistical Data Fusion
Authors: Ojo Adegbola , Papachristodoulou Nikolaos , Ibeh Samuel
Published: July 2018

Nigeria recently witnessed a decade of urban, economic, and social transformation. However, certain features of the Nigerian business environment act as constraints on economic growth. These characteristics also deter investment and exasperate business competitiveness. Inadequate infrastructure is identified as a major constraint to growth and competitiveness. This article summarises the technical development of a City Infrastructure Quality Index (CIQI) aimed at providing a relative measure of performance in urban infrastructure in Nigeria, focusing specifically on factors of production and resources for inclusive economic growth and development. The design of the CIQI adopts quality assurance principles undergirded by a five-stage systematic integration and calibration of spatial statistics derived from secondary data sources. The results have allowed for the piloting of a comparative framework for gauging good practice, excellence and adherence to recognisable standards in the provision of infrastructure across 37 Nigerian urban and metropolitan areas.
German Medical Informatics Initiative
Authors: Semler Sebastian , Wissing Frank , Heyder Ralf
Published: July 2018

This article is part of the Focus Theme of Methods of Information in Medicine on the German Medical Informatics Initiative. The Medical Informatics Initiative (MII) was launched within the scope of the German Federal Ministry of Education and Research’s (BMBF) Medical Informatics Funding Scheme, with the goal of developing infrastructure for the integration of clinical data from patient care and medical research in Germany. Its work is to be performed over the course of a decade (2016–2025) across three funding phases, with the first two concentrating on university hospitals. During the conceptual phase (now concluded), a central supporting project ensured coordination – and laid the ground for standardised solutions for all the initiative’s sites and scientific consortia that will enable effective data use and exchange, both for health care as well as research. The conceptual phase focused on the following: a) interoperability, through the consistent use of international standards (from an early stage, i.e. primary IT systems in patient care); b) standardised templates for patient consent and harmonised data protection; and c) standard rules for data use and access (monitoring and safeguarding access to data). On this basis, the initiative aims in the long term to improve medical research (particularly health care research, using data from treatments), to accelerate the transfer of knowledge from research to patient care – and to provide important impetus for the digitalization of medicine in Germany.
Overcoming Endocrine Resistance in Hormone Receptor–Positive Breast Cancer
Authors: AlFakeeh A. , Brezden-Masley C.
Published: July 2018

Endocrine therapy, a major modality in the treatment of hormone receptor (HR)–positive breast cancer (BCA), has improved outcomes in metastatic and nonmetastatic disease. However, a limiting factor to the use of endocrine therapy in BCA is resistance resulting from the development of escape pathways that promote the survival of cancer cells despite estrogen receptor (er)–targeted therapy. The resistance pathways involve extensive cross-talk between ER and receptor tyrosine kinase growth factors [epidermal growth factor receptor, human epidermal growth factor receptor 2 (HER2), and insulin-like growth factor 1 receptor] and their downstream signalling pathways—most notably pi3k/akt/mtor and mapk. In some cases, resistance develops as a result of genetic or epigenetic alterations in various components of the signalling pathways, such as overexpression of HER2 and ERα co-activators, aberrant expression of cell-cycle regulators, and PIK3CA mutations. By combining endocrine therapy with various molecularly targeted agents and signal transduction inhibitors, some success has been achieved in overcoming and modulating endocrine resistance in HR-positive BCA. Established strategies include selective ER downregulators, anti-HER2 agents, mtor (mechanistic target of rapamycin) inhibitors, and inhibitors of cyclin-dependent kinases 4 and 6. Inhibitors of pi3ka are not currently a treatment option for women with HR-positive BCA outside the context of clinical trial. Ongoing clinical trials are exploring more agents that could be combined with endocrine therapy, and biomarkers that would help to guide decision-making and maximize clinical efficacy. In this review article, we address current treatment strategies for endocrine resistance, and we highlight future therapeutic targets in the endocrine pathway of BCA.
ALK Inhibitors, Resistance Development, Clinical Trials
Authors: Rothenstein J.M. , Chooback N.
Published: July 2018

The treatment of advanced non-small-cell lung cancer (NSCLC) has undergone a paradigm shift since the early 2000s. The identification of molecular subtypes of the disease, based on oncogenic drivers, has led to the development of personalized medicine and the ability to deliver molecularly targeted therapies to patients. In the 10 years that have elapsed since the discovery of the ALK gene in a patient with NSCLC, several active drugs have moved rapidly from bench to bedside, and multiple others are currently in clinical trials. Those developments have led to important improvements in patient outcomes, while simultaneously raising key questions about the optimal treatment for ALK-positive NSCLC. The inevitable emergence of resistance to ALK-directed therapy is central to ongoing research and daily clinical practice for affected patients. In the present review, we highlight the current treatment landscape, the available and emerging clinical trials, and the evolving clinical decision-making in ALK-positive NSCLC, with a focus on Canadian practice.